bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urinary cortisol is lower in pregnant women with higher pre-pregnancy BMI
Authors: Hohman Emily E. , Smyth Joshua M. , McNitt Katherine M. , Pauley Abigail M. , Symons Downs Danielle , Savage Jennifer S. 
Published: January 2023

Background/objectivesAlthough cortisol levels increase during normal pregnancy, particularly high levels of cortisol or stress have been associated with adverse maternal/child outcomes. Obesity is associated with altered cortisol metabolism, but there is limited information on pregnancy-related changes in cortisol in pregnant women with overweight/obesity. The objective of this study was to examine weekly measures of urinary cortisol and perceived stress throughout ~10-36 weeks gestation, if levels differ by pre-pregnancy BMI categories, and whether concurrent measures of urinary cortisol and perceived stress are associated.MethodsLongitudinal observational data from Healthy Mom Zone, a gestational weight management intervention, and an ancillary fetal growth study were combined. Pregnant women with normal (n=7), overweight (n=11), or obese (n=14) pre-pregnancy BMI were recruited at &gt;8 weeks gestation. Overnight urinary cortisol and Perceived Stress Scale were measured weekly from ~10-36 weeks gestation.ResultsHigher pre-pregnancy BMI was associated with overall lower urinary cortisol throughout gestation, but rate of increase in urinary cortisol across pregnancy was similar across weight status groups. Women with obesity reported higher levels of overall perceived stress than normal weight women. Regardless of weight status, perceived stress was not associated with gestational age or cortisol.ConclusionsAlthough women with obesity reported higher perceived stress, they had lower urinary cortisol than women with normal BMI, and gestation-related increases in cortisol were similar across weight groups and unrelated to perceived stress, suggesting that physiological factors that drive increases in cortisol as pregnancy may outweigh effects of stress and adiposity.Clinical trial registrationhttps://clinicaltrials.gov/ct2/show/NCT03945266, identifier (NCT03945266)
Circulating apelin and chemerin levels in patients with polycystic ovary syndrome: A meta-analysis
Authors: Gao Yiming , Xin Caihong , Fan Huaying , Sun Xin , Wang Hongli 
Published: January 2023

BackgroundPolycystic ovary syndrome (PCOS) is one of the most common gynecological endocrine disorders. Apelin and chemerin are newly identified adipokines, which are higher in obesity and diabetes. Studies have found that the serum apelin and chemerin levels in patients with PCOS are significantly increased. However, other studies showed the opposite results. Therefore, the relationship between those two adipokines and PCOS is still controversial.AimThis meta-analysis was conducted to statistically evaluate the apelin and chemerin levels of patients with PCOS.MethodsWe searched the Web of Science, Embase, PubMed, and Google Scholar databases for potential studies. “Polycystic ovary syndrome” or “PCOS” in combination with the terms “apelin” or “chemerin” were used as keywords search titles or abstracts. The publication period examined was between 1990 and 2021. Standardized mean differences (SMD) with corresponding 95% confidence intervals (CIs) were determined as the results of the meta-analysis.ResultsA total of 148 articles were initially retrieved, and 18 qualified articles were finally obtained through preliminary screening and quality evaluation. The publications together contain 1,265 cases and 894 controls. The results of the meta-analysis showed that the circulating chemerin levels in patients with PCOS were significantly higher than those in the controls (SMD: 0.79, 95% CI [0.36, 1.23]), and there was no significant difference in circulating apelin between patients with PCOS and controls (SMD: 0.57, 95% CI [-0.21, 1.35]).ConclusionsThis meta-analysis is the first to evaluate circulating apelin and chemerin levels in patients with PCOS. Our findings suggest that circulating chemerin levels of patients with PCOS are significantly higher than those of healthy controls.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=218316, identifier CRD42020218316.
Sperm telomere length as a novel biomarker of male infertility and embryonic development: A systematic review and meta-analysis
Authors: Yuan Yacheng , Tan Yangyang , Qiu Xiaolong , Luo Hengfeng , Li Yuxiang , Li Ruijie , Yang Xukai 
Published: January 2023

BackgroundTelomeres have an essential role in maintaining the integrity and stability of the human chromosomal genome and preserving essential DNA biological functions. Several articles have been published on the association of STL with male semen parameters and clinical pregnancy. The results, however, are either inconclusive or inconsistent. Therefore, this meta-analysis aimed to systematically assess the accuracy and clinical value of sperm telomere length (STL) as a new marker for diagnosing male infertility and predicting the quality of embryonic development.MethodsWe performed a comprehensive systematic search for relevant publications in PubMed, the Cochrane Library, Web of Science, Embase, Scopus, and Ovid, from database build to August 2022. All experimental studies exploring the association of STL with male semen quality, male infertility, or embryonic development were included.ResultsOverall, Twelve prospective observational cohort studies (1700 patients) were eligible for inclusion in the meta-analysis. The meta-analysis showed a positive linear correlation between STL and semen parameters. The optimal cut-off value for STL diagnosing male infertility was 1.0, with a sensitivity and specificity of 80%. Regarding STL and embryonic development, the clinical pregnancy rate was associated with longer STL, and there was no significant difference between the two groups regarding fertilization rate.ConclusionOur study showed that STL has good diagnostic and predictive value for male fertility and clinical pregnancy and could be used as a new biomarker for diagnosing male infertility and predicting embryonic development.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022303333.
Safety analysis of representative accidental transients for a 100 MWe small modular LBE-cooled reactor
Authors: Chen Kang , Tang Chuntao , Qian Yalan , Lin Qian , Zhao Jinkun , Lei Yun 
Published: January 2023

Nuclear energy plays an important role in the green low-carbon energy. The lead-based reactor is one of the most popular small modular reactor types. The pre-conceptual design of a 100 MW(e) small modular lead-bismuth eutectic cooled reactor was developed by Shanghai Nuclear Engineering Research &amp; Design Institute CO., LTD. to meet the market demand of advanced small-scale nuclear plant. Representative accidents were analyzed for the 100 MW(e) small modular lead-bismuth reactor using ATHLET/MOD 3. The result shows that under the design basis conditions, the performance of the reactor can meet the safety criteria with the protection system working normally. The coolant solidification in the primary circuit under these transients should be prevented. Under the design extension condition, the highest cladding temperature is much lower than the melting point. In the unprotected reactivity insertion condition, there may be a short time of local melting of the fuel, which also meet the acceptance criteria. The safety analysis demonstrated the passive safety of the design with the negative temperature coefficient, strong natural circulation capability, and the passive residual heat removal system.
Mindful parenting—A thematic exploration of narratives from Indian mothers
Authors: Mazumdar Ketoki , Parekh Sneha , Sen Isha 
Published: January 2023

IntroductionWith the global crisis of COVID-19 continuing, Indian mothers have not received adequate attention with respect to their challenges and mothering experiences. The current study explored mindful parenting practices in a cohort of Indian mothers of children aged 10 years and below that emerged in response to the challenges posed by COVID-19.MethodsIn-depth virtual interviews were conducted with 31 urban Indian mothers to explore their lived experiences of mothering during the global crisis and their engagements with mindful parenting practices. The data were thematically analyzed.Results and DiscussionThe study identified two overarching themes and nine subthemes. The first theme, pandemic-induced stress, included the sub-themes of increased workload, poor support system, lack of time for self, and emotional and physical distress. The second theme of mindful parenting included the sub-themes of awareness as a mother and around the child, acceptance toward self and the child, empathic understanding of self and the child, active engagement with the child, and emotional regulation. Increased workload on all fronts coupled with poor support and a lack of time for self-contributed to exacerbated emotional and physical stress in mothers. They addressed these concerns posed by their lived experiences by engaging in mindful parenting processes in their mothering practices. Mindfulness-based cognitive therapy, mindfulness-based stress reduction, and mindfulness-based parenting techniques could be explored as possible interventions for mothers to alleviate their distress while drawing attention to larger structural changes and policy-level interventions addressing social issues such as gender inequality and childcare concerns.
Statins change the cytokine profile in Trypanosoma cruzi-infected U937 macrophages and murine cardiac tissue through Rho-associated kinases inhibition
Authors: González-Herrera Fabiola , Clayton Natasha S. , Guzmán-Rivera Daniela , Carrillo Ileana , Castillo Christian , Catalán Mabel , Anfossi Renatto , Quintero-Pertuz Helena , Quilaqueo María Elena , Olea-Azar Claudio , Rivera-Meza Mario , Kemmerling Ulrike , Ridley Anne J. , Vivar Raúl , Maya Juan Diego 
Published: January 2023

IntroductionChronic Chagasic cardiomyopathy (CCC), caused by the protozoan Trypanosoma cruzi, is the most severe manifestation of Chagas disease.CCC is characterized by cardiac inflammation and fibrosis caused by a persistent inflammatory response. Following infection, macrophages secrete inflammatory mediators such as IL-1β, IL-6, and TNF-α to control parasitemia. Although this response contains parasite infection, it causes damage to the heart tissue. Thus, the use of immunomodulators is a rational alternative to CCC. Rho-associated kinase (ROCK) 1 and 2 are RhoA-activated serine/threonine kinases that regulate the actomyosin cytoskeleton. Both ROCKs have been implicated in the polarization of macrophages towards an M1 (pro-inflammatory) phenotype. Statins are FDA-approved lipid-lowering drugs that reduce RhoA signaling by inhibiting geranylgeranyl pyrophosphate (GGPP) synthesis. This work aims to identify the effect of statins on U937 macrophage polarization and cardiac tissue inflammation and its relationship with ROCK activity during T. cruzi infection.MethodsPMA-induced, wild-type, GFP-, CA-ROCK1- and CA-ROCK2-expressing U937 macrophages were incubated with atorvastatin, or the inhibitors Y-27632, JSH-23, TAK-242, or C3 exoenzyme incubated with or without T. cruzi trypomastigotes for 30 min to evaluate the activity of ROCK and the M1 and M2 cytokine expression and secretion profiling. Also, ROCK activity was determined in T. cruzi-infected, BALB/c mice hearts.ResultsIn this study, we demonstrate for the first time in macrophages that incubation with T. cruzi leads to ROCK activation via the TLR4 pathway, which triggers NF-κB activation. Inhibition of ROCKs by Y-27632 prevents NF-κB activation and the expression and secretion of M1 markers, as does treatment with atorvastatin. Furthermore, we show that the effect of atorvastatin on the NF-kB pathway and cytokine secretion is mediated by ROCK. Finally, statin treatment decreased ROCK activation and expression, and the pro-inflammatory cytokine production, promoting anti-inflammatory cytokine expression in chronic chagasic mice hearts.ConclusionThese results suggest that the statin modulation of the inflammatory response due to ROCK inhibition is a potential pharmacological strategy to prevent cardiac inflammation in CCC.
Alterations of the gut virome in patients with systemic lupus erythematosus
Authors: Chen Changming , Yan Qiulong , Yao Xueming , Li Shenghui , Lv Qingbo , Wang Guangyang , Zhong Qin , Tang Fang , Liu Zhengqi , Huang Ying , An Yang , Zhou Jing , Zhang Qiongyu , Zhang Aiqin , Ullah Hayan , Zhang Yue , Liu Can , Zhu Dan , Li Hufan , Sun Wen , Ma Wukai 
Published: January 2023

BackgroundSystemic lupus erythematosus (SLE) is a systemic autoimmune disease that has been linked to the dysbiosis of the gut microbiome and virome. However, the potential characterization of the gut virome in SLE patients needs to be explored more extensively.MethodsHerein, we analyzed the gut viral community of 16 SLE patients and 31 healthy controls using both bulk and virus-like particle (VLP)-based metagenomic sequencing of their fecal samples. A total of 15,999 non-redundant viral operational taxonomic units (vOTUs) were identified from the metagenomic assembled contigs and used for gut virome profiling.ResultsSLE patients exhibited a significant decrease in gut viral diversity in the bulk metagenome dataset, but this change was not significant in the VLP metagenome dataset. Also, considerable alterations of the overall gut virome composition and remarkable changes in the viral family compositions were observed in SLE patients compared with healthy controls, as observed in both two technologies. We identified 408 vOTUs (177 SLE-enriched and 231 control-enriched) with significantly different relative abundances between patients and controls in the bulk virome, and 18 vOTUs (17 SLE-enriched in 1 control-enriched) in the VLP virome. The SLE-enriched vOTUs included numerous Siphoviridae, Microviridae, and crAss-like viruses and were frequently predicted to infect Bacteroides, Parabacteroides, and Ruminococcus_E, while the control-enriched contained numerous members of Siphoviridae and Myoviridae and were predicted to infect Prevotella and Lachnospirales_CAG-274. We explored the correlations between gut viruses and bacteria and found that some Lachnospirales_CAG-274 and Hungatella_A phages may play key roles in the virus-bacterium network. Furthermore, we explored the gut viral signatures for disease discrimination and achieved an area under the receiver operator characteristic curve (AUC) of above 0.95, suggesting the potential of the gut virome in the prediction of SLE.ConclusionOur findings demonstrated the alterations in viral diversity and taxonomic composition of the gut virome of SLE patients. Further research into the etiology of SLE and the gut viral community will open up new avenues for treating and preventing SLE and other autoimmune diseases.
Effect of 2-D nanomaterials on sensitivity of plasmonic biosensor for efficient urine glucose detection
Authors: Yadav Archana , Kumar Shatrughna , Kumar Anil , Sharan Preeta 
Published: January 2023

This paper presents a multi-layered Kretschmann configuration-based Surface Plasmon Resonance (SPR) biosensor for the detection of urine glucose. The modelling, simulation, and analysis have been done by using Silver (Ag) and Gold (Au) layer on the low refractive index prism BK-7 separately, which created two structures: structure-I (BK7/Ag/Bio-sample) and structure-II (BK7/Au/Bio-sample). Urine samples from a non-diabetic person (0–15 mg/dL) and a diabetic person (.625 gm/dL, 1.25 gm/dL, 2.5 gm/dL, 5 gm/dL, and 10 gm/dL) with the corresponding refractive indices of 1.335, 1.336, 1.337, 1.338, 1.341, and 1.347, respectively, have been used as a bio-sample that has been put on the top layer of the sensor. An investigation was conducted to improve the performance parameters of the proposed plasmonic biosensor by layering different 2-D nanomaterials (graphene, BP) and TMDC materials (MoS2, MoSe2, WS2, and WSe2) over the silicon (Si) layer in both structures at a visible wavelength of 633 nm, using Transfer Matrix Method (TMM). With layer thickness optimization, Structure-I (BK7/Ag (56 nm/Si (3 nm)/WS2 (.8 nm)/Bio-sample) shows a sensitivity of 200 °/RIU which is enhanced up to 1.7 times that of the conventional biosensor (BK7/Ag/Bio-sample) and 1.3 times that of the BK7/Ag (56 nm)/Si (3 nm)/Bio-sample based biosensor. Whereas in Structure-II (BK7/Au (50 nm)/Si (3 nm)/BP (.53 nm)/Bio-sample) with optimised layer thickness, we obtained a sensitivity of 273.4°/RIU, which is enhanced up to 2.2 times that of the conventional biosensor (BK7/Au/Bio-sample) and 1.3 times that of the BK7/Au (50 nm)/Si (3 nm)/Bio-sample. Other performance parameters such as detection accuracy for Structure-I and Structure-II are .5617 degree−1 and .134 degree−1 respectively. The Figure of merit for Structure-I and Structure-II are 112.35/RIU and 36.89/RIU respectively. Therefore, we expect Structure-I (BK7/Ag (56 nm/Si (3 nm)/WS2 (.8 nm)/Bio-sample) and Structure-II (BK7/Au (50 nm)/Si (3 nm)/BP (.53 nm)/Bio-sample) have the potential to detect the glucose concentration with quick response and high sensitivity in terms of the resonance angle shift in SPR curves.
The impact of pre-operative axial length on myopic shift 3 years after congenital and developmental cataract surgery and intraocular lens implantation
Authors: Zhou Xiyue , Fan Fan , Liu Xin , Yang Jianing , Yang Tianke , Luo Yi 
Published: January 2023

PurposeTo investigate the impact of the pre-operative axial length (AL) on myopic shift (MS) 3 years after primary intraocular lens (IOL) implantation in congenital/developmental cataract patients.MethodsA retrospective study of patients who underwent congenital/developmental cataract surgery and primary IOL implantation at age 2–3 years at EENT Hospital was conducted. All patients were followed up regularly for at least 3 years after surgery. Refractive outcomes, including spherical equivalent (SE) and MS, were collected at each follow-up.ResultsForty eyes from 40 patients were included. The mean age at surgery was 2.56 ± 0.57 years old, and the mean follow-up time was 3.05 ± 0.22 years. Patients were divided into two groups: Group 1 included 20 patients with longer pre-operative ALs (≥22 mm), and Group 2 included 20 patients with average pre-operative ALs (&lt;22 mm). By the last follow-up, the MS was 2.13 (0.38, 2.63) D in Group 1 and 3.88 (2.85, 5.72) D in Group 2. The post-operative MS in Group 2 was statistically greater than that in Group 1 at 3 years after surgery (P &lt; 0.001).ConclusionIn congenital/developmental cataract patients who underwent cataract extraction and primary IOL implantation at age 2–3 years, eyes with longer pre-operative ALs had a slower MS than those with average pre-operative ALs 3 years after surgery. This finding could have implications for the target refraction decision in congenital/developmental cataract surgery.
High clinical diagnostic accuracy of combined salivary gland and myocardial metaiodobenzylguanidine scintigraphy in the diagnosis of Parkinson’s disease
Authors: Li Shuangfang , Yue Lei , Chen Shuzhen , Wu Zhuang , Zhang Jingxing , Hong Ronghua , Xie Ludi , Peng Kangwen , Wang Chenghong , Lin Ao , Jin Lingjing , Guan Qiang 
Published: January 2023

BackgroundDecreased myocardial uptake of 131I-metaiodobenzylguanidine (MIBG) is known to be an important feature to diagnose Parkinson’s disease (PD). However, the diagnosis accuracy of myocardial MIBG scintigraphy alone is often unsatisfying. Recent studies have found that the MIBG uptake of the major salivary glands was reduced in PD patients as well.PurposeTo evaluate the diagnostic value of major salivary gland MIBG scintigraphy in PD, and explore the potential role of myocardial MIBG scintigraphy combined with salivary gland MIBG scintigraphy in distinguishing PD from non-PD (NPD).MethodsThirty-seven subjects were performed with 131I-MIBG scintigraphy. They were classified into the PD group (N = 18) and the NPD group (N = 19), based on clinical diagnostic criteria, DAT PET and 18F-FDG PET imaging findings. Images of salivary glands and myocardium were outlined to calculated the MIBG uptake ratios.ResultsThe combination of left parotid and left submandibular gland early images had a good performance in distinguishing PD from NPD, with sensitivity, specificity, and accuracy of 50.00, 94.74, and 72.37%, respectively. Combining the major salivary gland and myocardial scintigraphy results in the early period showed a good diagnostic value with AUC, sensitivity and specificity of 0.877, 77.78, and 94.74%, respectively. Meanwhile, in the delayed period yield an excellent diagnostic value with AUC, sensitivity and specificity of 0.904, 88.89, and 84.21%, respectively.Conclusion131I-MIBG salivary gland scintigraphy assisted in the diagnosis and differential diagnosis of PD. The combination of major salivary gland and myocardial 131I-MIBG scintigraphy further increased the accuracy of PD diagnosis.
A case report: New-onset refractory status epilepticus in a patient with FASTKD2-related mitochondrial disease
Authors: Astner-Rohracher Alexandra , Mauritz Matthias , Leitinger Markus , Rossini Fabio , Kalss Gudrun , Neuray Caroline , Retter Elisabeth , Wortmann Saskia B. , Achleitner Melanie T. , Mayr Johannes A. , Trinka Eugen 
Published: January 2023

ObjectivesNew-onset refractory status epilepticus (NORSE) is associated with high morbidity and mortality. Despite extensive work-up, the underlying etiology remains unknown in 50% of affected individuals. Mitochondrial disorders represent rare causes of NORSE. Biallelic variants in FASTKD2 were reported as a cause of infantile encephalomyopathy with refractory epilepsy.Case descriptionIn the study, we report a previously healthy 14-year-old with a new, homozygous FASTKD2 variant presenting with NORSE. Following a seizure-free period of 7 years, he experienced another super-refractory SE and subsequently developed drug-resistant focal epilepsy, mild myopathy, optic atrophy, and discrete psychomotor slowing. Structural MRI at the time of NORSE showed right temporo-parieto-occipital FLAIR hyperintensity and diffusion restriction, with extensive right hemispheric atrophy at the age of 22 years. Whole-exome sequencing revealed a novel homozygous loss of function variant [c.(1072C&gt;T);(1072C&gt;T)] [p.(Arg358Ter);(Arg358Ter)] in FASTKD2 (NM_001136193), resulting in a premature termination codon in the protein-coding region and loss of function of FASTKD2. Oxidative phosphorylation (OXPHOS) in muscle and skin fibroblasts was unremarkable.ConclusionThis is the first case of a normally developed adolescent with a new homozygous loss of function variant in FASTKD2, manifesting with NORSE. The phenotypical spectrum of FASTKD2-related mitochondrial disease is heterogeneous, ranging from recurrent status epilepticus and refractory focal epilepsy in an adolescent with normal cognitive development to severe forms of infantile mitochondrial encephalopathy. Although mitochondrial diseases are rare causes of NORSE, clinical features such as young age at onset and multi-system involvement should trigger genetic testing. Early diagnosis is essential for counseling and treatment considerations.