bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of environmental factors on two prominent diseases prevalent in New South Wales, Australia
Authors: Dissanayake Gnanadarsha Sanjaya , Duck Gerard 
Published: March 2023

Abstract

Background: Changing disease prevalence over time is an important factor in projecting for future health system requirements. Case studies and analysis of the impact of environmental and climate change on diseases affecting humans have been done in various parts of the world, providing a range of data points and interpretations. A unique subset of the published literature are statistical causality studies that infer the impact of certain environmental factors on incidence frequencies of some diseases that afflict humans. Occurrence of such frequencies in a periodic manner generate time series that could be utilised to develop future forecast predictions known as projections.
Methods: This study uses the Granger’s statistical causality method to assess the relationship between environmental variables and the prevalence of two diseases (Asthma and Melanoma) and presents projections using appropriate time series methodology to inform the health service planning requirements of the state of New South Wales (NSW).
Results: Causality between Air quality and Asthma incidence as well as Solar irradiance and Melanoma incidence are established through statistical significance measures as a contribution. Thereafter time series based forecast projections are provided for both the diseases as another contribution.
Conclusion: Statistical causality between environmental and health disease related linked variables have been established for Asthma and Melanoma, and subsequently disease incidence frequency projections in the form of point and interval estimates for the NSW state in Australia have been provided.
Identifying metastatic biomarkers of colorectal cancer: Machine learning modeling and experimental validation
Authors: Ahmadieh-Yazdi Amirhossein , Mahdavinezhad Ali , Tapak Leili , Nouri Fatemeh , Taherkhani Amir , Afshar Saeid 
Published: March 2023

Abstract
Background
Colorectal cancer (CRC) liver metastasis is responsible for the majority of CRC-related deaths. Early detection of metastasis is crucial for improving patient outcomes but can be delayed due to a lack of symptoms. In this research, we aimed to investigate for CRC metastasis related biomarkers by employing machine learning (ML) approach and experimental validation.
Methods
Gene expression profile of CRC patients with liver metastasis was obtained using GSE41568 dataset and the differentially expressed genes between primary and metastatic samples were screened. Subsequently, we carried out feature selection to identify most relevant DEGs using LASSO and Penalized-SVM methods. DEGs commonly selected by these methods were selected for further analysis. Finally, the experimental validation was done through qRT-PCR.
Results
11 genes were commonly selected by SCAD and P-SVM algorithms among which seven had prognostic value in colorectal cancer. It was found that the expression of MMP3 gene decreases in stage IV of colorectal cancer compared to other stages (p-value < 0.01). Also, the expression of WNT11gene increases significantly in this stage(p-value < 0.001). It was also found that expression of WNT5a, TNFSF11 and MMP3 is significantly lower, and the expression level of WNT11 is significantly higher in liver metastasis samples compared to primary tumors.
Conclusion
In summary, this study has identified a set of potential biomarkers for CRC metastasis using ML algorithms. The findings of this research may provide new insights into the identification of biomarkers for CRC metastasis and may lead to new therapeutic strategies for the treatment of this disease.
Downregulation of ubiquitin-specific protease 15 (USP15) does not provide therapeutic benefit in experimental mesial temporal lobe epilepsy
Authors: Häussler Ute , Neres João , Vandenplas Catherine , Eykens Caroline , Kadiu Irena , Schramm Carolin , Fleurance Renaud , Stanley Phil , Godard Patrice , de Mot Laurane , Eyll Jonathan van , Knobeloch Klaus-Peter , Haas Carola A. , Dedeurwaerdere Stefanie 
Published: March 2023

Abstract
Structural epilepsies display complex immune activation signatures; however, it is unclear which neuroinflammatory pathways drive disease pathobiology. Transcriptome studies of brain resections from mesial temporal lobe epilepsy (mTLE) patients revealed a dysregulation of transforming growth factor β, interferon α/β and nuclear factor erythroid 2-related factor 2 pathways among other neuroinflammatory mechanisms. Since these pathways are regulated by ubiquitin-specific proteases (USP), in particular USP15, we hypothesized that USP15 blockade may provide therapeutic relief in treatment-resistant epilepsies. For validation, transgenic mice which either constitutively or inducibly lack USP15 underwent intrahippocampal kainate injections to induce mTLE and to investigate the impact of USP15 downregulation at the molecular and phenotypic levels. We show that the severity of status epilepticus is unaltered in mice constitutively lacking Usp15 compared to wildtype littermates. Cell death, reactive gliosis and changes in the inflammatory transcriptome were pronounced at 4 days after kainate injection. However, the lack of USP15 did not alter brain inflammation signatures. Likewise, induced deletion of Usp15 in chronic epilepsy neither affected seizure generation, nor cell death, gliosis or the transcriptome. Concordantly, siRNA-mediated knockdown of Usp15 in a microglial cell line did not impact inflammatory responses in form of cytokine release. Our data show that a lack of USP15 is insufficient to modulate the expression of relevant neuroinflammatory pathways in mTLE and has no impact on epileptic activity in a mouse model. Although previous reports implicated a checkpoint function for USP15 in inflammation, our results do not support targeting USP15 as a therapeutic approach for pharmacoresistant epilepsy.
Square-Wave Voltammetry of Human Blood Serum
Authors: Kokoskarova Pavlinka , Stojanov Leon , Najkov Kosta , Ristovska Natasa , Ruskovska Tatjana , Skrzypek Sławomira , Mirceski Valentin 
Published: March 2023

Abstract
A study on voltammetric analysis of whole blood serum is presented using advanced square-wave voltammetry at an edge plane pyrolytic graphite electrode. The results demonstrate that even in a complex medium like human blood serum, electrochemical characterization can be achieved through the use of advanced voltammetric techniques in conjunction with an appropriate commercially available electrode, such as the edge plane pyrolytic graphite electrode, which boasts superior electrocatalytic properties. Without undergoing any chemical treatment of the serum sample, the square-wave voltammetry technique reveals, for the first time, the electrode reactions of uric acid, bilirubin, and albumin in a single experiment, as represented by well-defined, separated, and intense voltammetric signals. All electrode processes are surface-confined, indicating that the edge plane sites of the electrode serve as an ideal platform for the competitive adsorption of electroactive species, despite the extensive chemical complexity of the serum samples. The speed and differential nature of square-wave voltammetry are crucial for obtaining an outstanding resolution of the voltammetric peaks, maintaining the quasi-reversible nature of the underlying electrode processes, while reducing the impact of follow-up chemical reactions that are coupled to the initial electron transfer for all three detected species, and minimizing fouling of the electrode surface.
Determining what happens to the genes FOXP3, RORγt, SOCS1, STAT3, STAT5, and SMAD3 in patients with ankylosing spondylitis who received anti-TNF therapy
Authors: Aldaoseri Husham A. , Ali Naael Hussein , Al-Mubarak Fires 
Published: March 2023

Abstract
Background/Objective: Ankylosing spondylitis (AS) is a common inflammatory rheumatic disease that affects the axial skeleton, causing characteristic inflammatory back pain. We aim to examine the genes FOXP3, RORγt, SOCS1, STAT3, STAT5, and SMAD3 in patients with ankylosing spondylitis who receive anti-TNF therapy to understand the progression of the disease in a clinically improved or failed response to anti-TNF treatment.
Method: A prospective case-control study was conducted at Basrah Teaching Hospital in southern Iraq. Eighty-one AS patients were divided into two groups: 67 were treated with anti-TNF therapy; 14 were newly diagnosed patients as positive controls, and 65 were healthy individuals. Disease activity was assessed using the AS Bath Disease Activity Index (BASDAI). Conventional radiography and MRI are used to measure the severity of the disease. Blood samples were homogenized, and mRNA was isolated using a total RNA extraction kit. Reverse transcription (RT) was performed. The gene expression of RORγt, FOXP3, SOCS1, SAMD1, SMAD3, STAT3, and STAT5 was detected by implementing a real-timePCR system.
Result: There was significant downregulation of the FOXP3 gene in anti-TNF-treated AS patients and biologically naïve AS patients compared to healthy control people, which may be due to how the disease affects the expression and activity of the FOXP3 gene, which does not change with the type of therapy (P = 0.001). RORγt expression was higher in both established and newly diagnosed AS patients (p 0.001) than in healthy controls. The SOCS1 gene was expressed at a low level in patients with AS and positive control patients compared to the SOCS1 gene in healthy control individuals (p 0.780). STAT3 expression was lower in established and biologically naïve AS patients than in healthy subjects. Simultaneously, biologically normal AS patients had higher levels of STAT5 in this group. SMAD3 was underexpressed in established and biologically naïve AS patients compared to healthy subjects.
Conclusion: The progression of AS in patients treated with anti-TNF therapy is linked to a secondary change in the expression of the genes FOXP3, RORγt, SOCS1, STAT3, STAT5, and SMAD3 that leads to the induction of exTh17cells.
Association of social support before ICU admission with post-discharge mental health symptoms in ICU patients: A prospective cohort study
Authors: Yoshino Yasuyo , Unoki Takeshi , Hata Kimiko , Ito Kiyoe 
Published: March 2023

Abstract

Mental health problems after discharge from ICU interfere with physical recovery and seriously affect daily life. Social support has been suggested to be associated with mental health but has not been sufficiently characterized. This study aimed to evaluate the association of social support before ICU admission with mental health after ICU discharge. In this prospective cohort study, patients who admitted to the ICU for more than 48 h were surveyed on social support prior to ICU admission, and 3 months after discharge from the ICU, mental health questionnaires were mailed. 153 patients were enrolled; the prevalence of PTSD, anxiety, and depression, 3 months after discharge from the ICU was 11.3, 14.0, and 24.6%, respectively. Multivariate analysis using linear regression models adjusted for confounding by age, sex, and years of education for PTSD, anxiety, and depression revealed that social support women were independent factors associated with depressive symptoms. Additionally, gender differences were observed in depressive symptoms and social support associations. Social support prior to ICU admission was not associated with PTSD symptoms after ICU discharge. Higher social support before ICU admission may be associated with lower depressive symptoms after ICU discharge. It is important to provide necessary social support when needed.
Evaluation of the Effects of processing on Physicochemical Properties of the Freshly Extracted Crude Groundnut Oil
Authors: Afolabi Funmilayo Patricia , Ogundele Olamide Rachael , Raji Rofiat Oyebisi , Aderogba Mutalib Adeniran , Falade Olumuyiwa Sunday 
Published: March 2023

Abstract
The effect of processing on physicochemical properties of freshly extracted crude groundnut oil was determined. Crude groundnut oil was extracted following the traditional method and half of it was processed as done industrially. Chemical properties [acid value, free fatty acid, peroxide value, saponification value, ester value, iodine value, total phenol (TP), p-Anisidine value, total tocopherol (TT), and total antioxidant capacity (TAC) and thiobarbituric acid reactive species (TBARS)] and physical properties (refractive index, surface tension, smoke point, flash point, viscosity and specific gravity) were determined following the AOAC and other standard methods. Analysis of the results indicated that the processing of the crude groundnut oil, although improved the physical appearance and reduced free fatty acid by 56% hence, could improve its stability but it also compromised the levels of antioxidant compounds such as tocopherols in the oil that reduced by 16.4%. TAC, an antioxidant parameter also reduced by 92% while TBARS increased by 15.1%. It can be concluded that processing of groundnut oil could either improve its stability or compromise it, hence, there is need for the stability study of processed groundnut oil.
Quercetin-loaded Solid Lipid Nanoparticles Effectively Inhibit the Growth of Invasive Breast Cancer Cell Line MDA-MB 231
Authors: Hatami Mahdi , Kouchak Maryam ,   Alireza Kheirollah2, Khorsandi Layasadat , Rashidi Mojtaba 
Published: March 2023

Abstract

Background: Quercetin (QC) is a natural flavonoid abundant in fruits and vegetables. The anticancer and anti-inflammatory effects of quercetin have been reported previously, but the clinical application has been limited because of its low bioavailability. Triple-negative breast cancer is a type of breast cancer that responds poorly to chemotherapy. This study aims to determine the anti-cancer impact of quercetin-solid lipid nanoparticles (QC-SLN) on the triple-negative breast cancer cell line MDA-MB231.
Materials and Methods: Following a 48-hour treatment with 18.9 µM of QC and QC-SLN for MCF-7 and 13.4 µM for MDA-MB231, cell viability, apoptosis, the colony formation assay, and the anti-angiogenic effect of treatment were assessed.
Results: QC-SLN was constructed with the best properties (particle size of 154 nm, zeta potential of -27.7 mV, and encapsulation efficacy of 99.6%) and continuously released QC in 72 h .in QC-SLN group compared to the QC group, There was a significant decrease in cell viability, colony formation, angiogenesis and in addition, a significant increase in apoptosis, throughout modulation in Bax and Bcl-2 at gene and protein level.
Conclusions: Our findings indicated that SLN enhanced the cytotoxic effect of QC in MDA-MB231 cells by improving bioavailability and apoptotic intrinsic pathways. It may be a promising therapy for the future, but more in vivo study is needed.
Relationship between sarcopenia and cachexia with prognostic markers of middle-aged and older inpatients with COVID-19: a case-control study
Authors: Júnior José Reginaldo Alves de Queiroz , Pereira Jarson Pedro da Costa , Benjamim Raquel de Arruda Campos , Silva Nahara Oliveira Lima da , Silva Maria Eduarda de Paiva , Ramiro Claudia Porto Sabino Pinho 
Published: March 2023

Abstract
Purpose:SARS-CoV-2 infection can lead to various manifestations beyond an inflammatory response, such as anorexia, hyposmia, and other symptoms that may increase the risk of nutritional disorders. Sarcopenia and cachexia (nutritional disorders) are conditions that appear to influence COVID-19 evolution. Thus, this study aimed to evaluate sarcopenia and cachexia in hospitalized patients with COVID-19, verifying their clinical impacts and relationship with prognostic markers.
Methods: This is a case-control study involving inpatients with and without a COVID-19 diagnosis. The occurrence of sarcopenia was evaluated according to European Working Group on Sarcopenia 2 criteria, and cachexia was also evaluated. Inflammatory markers and the 4C Mortality Score were evaluated.
Results: The groups were similar regarding age (p = 0.656), sex (p = 0.777), presence of comorbidities (type 2 diabetes p = 0.659; systemic arterial hypertension p = 0.427), and Body Mass Index (p = 0.657). The frequency of sarcopenia and cachexia in patients with COVID-19 was 21.9% and 28.1%, respectively. Sarcopenic patients with COVID-19 had a higher risk of death (4C Mortality Score) (p = 0.038). The occurrence of sarcopenia or cachexia within the COVID-19 group was not associated with inflammatory biomarkers or a higher number of COVID-19 symptoms (p > 0.05).
Conclusion: The presence of sarcopenia among COVID-19 patients increased the risk of mortality.
Common Variable Immunodeficiency Patient Fecal Microbiota Transplant Recapitulates Gut Dysbiosis
Authors: Hajjar Joud , Voigt Anita , Conner Margaret , Swennes Alton , Fowler Stephanie , Calarge Chadi , Mendonca Danielle , Armstrong Dominique , Chang Cheng-Yen , Walter Jolan , Butte Manish , Savidge Tor , Oh Julia , Kheradmand Farrah , Petrosino Joseph 
Published: March 2023

Abstract
Purpose
Patients with non-infectious complications have worse clinical outcomes in common variable immunodeficiency (CVID) than those with infections-only. Non-infectious complications are associated with gut microbiome aberrations, but there are no reductionist animal models that emulate CVID. Our aim in this study was to uncover potential microbiome roles in the development of non-infectious complications in CVID.
Methods
We examined fecal whole genome shotgun sequencing from patients CVID, and non-infectious complications, infections-only, and their household controls. We also performed Fecal Microbiota transplant from CVID patients to Germ-Free Mice.
Results
We found potentially pathogenic microbes Streptococcus parasanguinis and Erysipelatoclostridium ramosum were enriched in gut microbiomes of CVID patients with non-infectious complications. In contrast, Fusicatenibacter saccharivorans and Anaerostipes hadrus, known to suppress inflammation and promote healthy metabolism, were enriched in gut microbiomes of infections-only CVID patients. Fecal microbiota transplant from non-infectious complications, infections-only, and their household controls into germ-free mice revealed gut dysbiosis patterns in recipients from CVID patients with non-infectious complications, but not infections-only CVID, or household controls recipients.
Conclusion
Our findings provide a proof of concept that fecal microbiota transplant from CVID patients with non-infectious complications to Germ-Free mice recapitulates microbiome alterations observed in the donors.
Computational modeling of AMPK and mTOR crosstalk in glutamatergic synapse calcium signaling
Authors: Rangamani Padmini , Leung Allen 
Published: March 2023

Abstract
Neuronal energy consumption is vital for information processing and memory formation in synapses. The brain consists of just 2% of the human body’s mass, but consumes almost 20% of the body’s energy budget. Most of this energy is attributed to active transport in ion signaling, with calcium being the canonical second messenger of synaptic transmission. Here, we develop a computational model of synaptic signaling resulting in the activation of two protein kinases critical in metabolic regulation and cell fate, AMP-Activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) and investigate the effect of glutamate stimulus frequency on their dynamics. Our model predicts that frequencies of glutamate stimulus over 10 Hz perturb AMPK and mTOR oscillations at higher magnitudes by up to 70% and area under curve (AUC) by 10%. This dynamic difference in AMPK and mTOR activation trajectories potentially differentiates high frequency stimulus bursts from basal neuronal signaling leading to a downstream change in synaptic plasticity. Further, we also investigate the crosstalk between insulin receptor and calcium signaling on AMPK and mTOR activation and predict that the pathways demonstrate multistability dependent on strength of insulin signaling and metabolic consumption rate. Our predictions have implications for improving our understanding of neuronal metabolism, synaptic pruning, and synaptic plasticity.
Methylene Orange and Methyl Blue Adsorption Behavior on Pine Leaves Biomass (Pinus kesiya)
Authors: Huynh Phuong Thao , Nguyen Duy-Khoi , Duong Bich-Ngoc , Nguyen Phi-Ho , Dinh Van-Phuc 
Published: March 2023

Abstract
The uptake of Methyl Orange (MO) and Methylene Blue (MB) from aqueous solutions onto Pine leaves (Pinus kesiya) was investigated in this work. Factors including pH solution, contact time, initial dye concentration were discovered to be relevant in the removal of dyes. Among four isotherm models (Langmuir, Sips, Freundlich, and Temkin), the experimental data was fitted the Langmuir model better than others. For MO and MB, the maximum Langmuir adsorption capacities were 136.99 mg.g − 1 and 140.85 mg.g − 1 , respectively. The kinetic studies demonstrated that the biosorption of MO and MB onto biomass of pine leaves was compatible with Elovich, pseudo-first-order, pseudo-second-order and intra-particle diffusion models. The thermodynamic studies showed that the uptake of the two dyes was regulated by physisorption, spontaneous, and endothermic in nature. Electrostatic interactions, as well as other non-covalent forces such as π–π interactions and hydrogen bonds, are mechanisms of dyes adsorption on pine leaf biomass. The current study found that pine leaves (Pinus kesiya) might be a potential biosorbent for the wastewater treatment due to their high availability and production, resulting in various environmental advantages.
The role of OXGR1 on gut smooth muscle to regulate intestinal motility and health
Authors: Xu Guli , Zhou Jingjing , Gyawali Ishwari , Feng Jinlong , Yuan Yexian , Xu Chang , Yang Jinping , Ma Zewei , Li Penglin , Sui Chengrong , Jiang Qingyan , Shu Gang , Zhu Canjun 
Published: March 2023

Abstract
Background
A stable intestinal microenvironment determines the balance of intestinal flora and body health, which is influenced by intestinal motility. 2-Oxoglutarate receptor 1 (OXGR1) is a G-protein-coupled receptor whose endogenous ligand is the resistance exercise metabolic intermediate α-ketoglutarate (AKG). Here, we aimed to determine the function of OXGR1 in intestinal motility, which modulate intestinal microenvironment.
Results
Through intestinal co-location, we found OXGR1 was highly expressed in intestinal smooth muscle. The model of OXGR1 lose-of-function mice showed enhanced intestinal barrier and motility, as well as improved intestinal flora. We further found that in gastrointestinal smooth muscle cells (GSMCs), AKG downregulates Car2 expression and modulates cellular acid-base balance by OXGR1, ultimately leading to the relaxation and reduction of motility of intestinal smooth muscle.
Conclusions
OXGR1 deletion improves intestinal microenvironment by enhanced intestinal motility; and these results provide a new target for intestinal motility regulation, OXGR1 may be developed for the treatment of some diseases such as microbiota disorders and enteritis.
Electrochemical property based on the structural control of pitch-based carbon anode
Authors: HWANG JINUNG , Lee Jong dae , Im Ji Sun 
Published: March 2023

AbstractThe charge/discharge characteristics of a carbon anode is studied in a lithium ion battery system based on the effects of a graphite structure developed by heat energy. A carbon precursor (pitch) is synthesized from pyrolysis of fuel oil, after which a carbonization/graphitization treatment at 1000–2400 ℃ is carried out on the carbon anode. The lithium storage mechanism of carbonized materials treated under 1300 ℃ is noted not by the space between the graphite layers but the cavity in the carbon materials. This indicates higher capacity and high speed charge-discharge performance capabilities than graphite. For graphitized materials treated at 2400 ℃, lithium ions are mainly inserted between the layers of the graphite, forming an interlayer structure. Based on the suggested mechanisms, it is noted that the charge/discharge of lithium ions can be controlled based on control of the graphite structure.