bluuebunny/crossref_metadata_2025_split huggingface dataset:
Classification molecular subtypes of hepatocellular carcinoma based on PRMT-related genes
Authors: Liu Liwen , Hu Qiuyue , Zhang Yize , Sun Xiangyi , Sun Ranran , Ren Zhigang 
Published: February 2023

Background: Recent studies highlighted the functional role of protein arginine methyltransferases (PRMTs) catalyzing the methylation of protein arginine in malignant progression of various tumors. Stratification the subtypes of hepatocellular carcinoma (HCC) is fundamental for exploring effective treatment strategies. Here, we aim to conduct a comprehensive analysis of PRMTs with bioinformatic tools to identify novel biomarkers for HCC subtypes classification and prognosis prediction, which may be potential ideal targets for therapeutic intervention.Methods: The expression profiling of PRMTs in HCC tissues was evaluated based on the data of TCGA-LIHC cohort, and further validated in HCC TMA cohort and HCC cell lines. HCC was systematically classified based on PRMT family related genes. Subsequently, the differentially expressed genes (DEGs) between molecular subtypes were identified, and prognostic risk model were constructed using least absolute shrinkage and selection operator (LASSO) and Cox regression analysis to evaluate the prognosis, gene mutation, clinical features, immunophenotype, immunotherapeutic effect and antineoplastic drug sensitivity of HCC.Results: PRMTs expression was markedly altered both in HCC tissues and HCC cell lines. Three molecular subtypes with distinct immunophenotype were generated. 11 PRMT-related genes were enrolled to establish prognostic model, which presented with high accuracy in predicting the prognosis of two risk groups in the training, validation, and immunotherapy cohort, respectively. Additionally, the two risk groups showed significant difference in immunotherapeutic efficacy. Further, the sensitivity of 72 anticancer drugs was identified using prognostic risk model.Conclusion: In summary, our findings stratified HCC into three subtypes based on the PRMT-related genes. The prognostic model established in this work provide novel insights into the exploration of related therapeutic approaches in treating HCC.
3.2 kW, 0.22 nm narrow-linewidth MOPA configuration fiber laser with a homemade polarization-maintaining Yb-doped fiber
Authors: Liao Shibiao , Luo Tao , Xiao Runheng , Cheng Junjie , Shu Chang , Zhang Zhilun , Zhou Yanyan , Xing Yingbin , Li Haiqing , Peng Jinggang , Dai Nengli , Li Jinyan 
Published: February 2023

In this work, a narrow-linewidth linearly polarized fiber amplifier with a record output power of 3.2 kW was achieved based on a homemade polarization-maintaining Yb-doped fiber corresponding to a slope efficiency of 79% and a 3 dB linewidth of 0.2227 nm. By examining various numerical aperture (NA) PMYDFs, the experimental investigation on expanding mode instability (MI) threshold in PM fiber amplifiers was put on display. And the results reveal that the MI threshold is enhanced by more than 370 W for every 0.004 decrease in core numerical aperture. Increasing the seed linewidth from 0.0454 nm to 0.0976 nm by adding 200 m polarization maintaining Ge-doped fiber the stimulated Brillouin scattering threshold increased from 805 W to above 3.2 kW. By applying the MI suppression method, a double-eight-shaped aluminum plate was adopted to coil the gain fiber, and the MI threshold increased by more than 1100 W.
Fungal identification in peanuts seeds through multispectral images: Technological advances to enhance sanitary quality
Authors: Sudki Julia Marconato , Fonseca de Oliveira Gustavo Roberto , de Medeiros André Dantas , Mastrangelo Thiago , Arthur Valter , Amaral da Silva Edvaldo Aparecido , Mastrangelo Clíssia Barboza 
Published: February 2023

The sanitary quality of seed is essential in agriculture. This is because pathogenic fungi compromise seed physiological quality and prevent the formation of plants in the field, which causes losses to farmers. Multispectral images technologies coupled with machine learning algorithms can optimize the identification of healthy peanut seeds, greatly improving the sanitary quality. The objective was to verify whether multispectral images technologies and artificial intelligence tools are effective for discriminating pathogenic fungi in tropical peanut seeds. For this purpose, dry peanut seeds infected by fungi (A. flavus, A. niger, Penicillium sp., and Rhizopus sp.) were used to acquire images at different wavelengths (365 to 970 nm). Multispectral markers of peanut seed health quality were found. The incubation period of 216 h was the one that most contributed to discriminating healthy seeds from those containing fungi through multispectral images. Texture (Percent Run), color (CIELab L*) and reflectance (490 nm) were highly effective in discriminating the sanitary quality of peanut seeds. Machine learning algorithms (LDA, MLP, RF, and SVM) demonstrated high accuracy in autonomous detection of seed health status (90 to 100%). Thus, multispectral images coupled with machine learning algorithms are effective for screening peanut seeds with superior sanitary quality.
The causal role of gastroesophageal reflux disease in anxiety disorders and depression: A bidirectional Mendelian randomization study
Authors: Zeng Youjie , Cao Si , Yang Heng 
Published: February 2023

BackgroundObservational studies have shown an association between gastroesophageal reflux disease (GERD) and anxiety disorders/depression. However, these evidences may be influenced by confounding factors. Therefore, our study aimed to determine the causal relationship between GERD and anxiety disorders/depression by conducting a bidirectional Mendelian randomization (MR) study.MethodsWe performed a bidirectional MR analysis using summary statistics from genome-wide association studies (GWAS) in European individuals. The inverse-variance weighted (IVW) method was used as the primary analytical method to assess causality. In addition, five additional MR methods [maximum likelihood, MR-Egger, weighted median, robust adjusted profile score (MR-RAPS), and mode-based estimate (MR-MBE)] were performed to supplement the IVW results. Furthermore, several sensitivity analyses were performed to assess heterogeneity, horizontal pleiotropy, and stability. Finally, a multivariable MR (MVMR) analysis was performed to determine the causal relationship by adjusting for potential confounders.ResultsMR results of the IVW method indicated that GERD significantly increases the risk of anxiety disorders [odds ratio (OR) = 1.35, 95% confidence interval (CI) 1.15–1.59, P = 2.25 × 10–4] and depression (OR = 1.32, 95% CI: 1.15–1.52, P = 1.26 × 10–4). In addition, the MR results of maximum likelihood, MR-Egger, weighted median, MR-RAPS, and MR-MBE remained parallel to the IVW results. Furthermore, sensitivity analysis suggested that the results were robust, with no pleiotropy or heterogeneity detected. Nevertheless, reverse MR analysis showed that anxiety or depression did not increase GERD risk. Finally, MVMR analysis showed that the effect of GERD on increasing the risk of anxiety disorders/depression was independent of confounders.ConclusionThis MR study supports a causal association between GERD and an increased risk of anxiety disorders and depression. Therefore, complementing symptomatic treatment of GERD with psychological assessment and necessary psychological support therapy may help reduce the risk of future anxiety disorders and depression.
Age at SARS-CoV-2 infection and psychological and physical recovery among Chinese health care workers with severe COVID-19 at 28 months after discharge: A cohort study
Authors: Li Qian , Xiong Lijuan , Cao Xiongjing , Xiong Huangguo , Zhang Yanzhao , Fan Yunzhou , Tang Liang , Jin Yang , Xia Jiahong , Hu Yu 
Published: February 2023

BackgroundNo prior study had reported the psychological and physical recovery of patients with COVID-19 2~3 years after discharge from the hospital. Moreover, it is not clear whether there is any difference in the health status of the patients with COVID-19 of different ages after discharge from the hospital.MethodsEmbedding in the “Rehabilitation Care Project for Medical Staff Infected with COVID-19” in China, this study included 271 health care workers (HCWs) with severe COVID-19. Their status of health-related quality of life, persistent symptoms, functional fitness and immune function at 28 months after discharge were followed, and compared according to tertiles of age at SARS-CoV-2 infection (group of younger (≤ 33 years); medium (34-42 years); and older (≥43 years)). Multivariate linear regression and multivariable adjusted logistic regression models were applied in investigating the associations of age at SARS-CoV-2 infection and outcomes.ResultsAt 28 months after discharge, 76% of the HCWs with severe COVID-19 had symptom of fatigue/weakness; 18.7% of the HCWs with severe COVID-19 did not fully recover their functional fitness; the decrease of CD3+ T cells, CD8+ T cells and the increase of natural killer cells accounted for 6.6, 6.6, and 5.5%, respectively. Compared with the HCWs with severe COVID-19 in younger group, HCWs with severe COVID-19 in older group had lower scores regarding physical functioning, role physical, bodily pain and role emotional; HCWs with severe COVID-19 in older group had higher risk of cough, joint pain, hearing loss and sleep disorder; HCWs with severe COVID-19 in older group scored lower on flexibility test. The variance of relative numbers of CD3+ T cells, CD8+ T cells and natural killer cells among HCWs with severe COVID-19 of different age groups were significant.ConclusionsThis study demonstrated that older HCWs with severe COVID-19 recovered slower than those with younger age regarding health-related quality of life, persistent symptoms, functional fitness and immune function at 28 months after discharge. Effective exercise interventions regarding flexibility should be performed timely to speed their rehabilitation, especially among those with older age.
Patient satisfaction impact indicators from a psychosocial perspective
Authors: Wang Yao , Liu Chenchen , Wang Pei 
Published: February 2023

BackgroundPatient satisfaction plays an important role in improving patient behavior from care, reducing healthcare costs, and improving outcomes. However, since patient satisfaction is a multidimensional concept, it remains unclear which factors are the key indicators of patient satisfaction. The purpose of this study was to verify whether and how patients' psychosocial perceptions of physicians influenced patient satisfaction.MethodIn China, 2,256 patients were surveyed on stereotypes of physicians, institutional trust, humanized perception, and communication skills, as well as patient expectations and patient satisfaction. The data were analyzed using structural equation modeling.ResultsStereotypes, institutional trust, and humanized perception have an indirect effect on patient satisfaction through communication, and patient expectations have a direct effect on patient satisfaction.Conclusions“Patient-centered” communication is the key to improving patient satisfaction, while positive stereotypes at the societal level, standardization of organizational institutions, expression of the doctor's view of humanity in the doctor-patient interaction, and reasonable guidance of patient expectations are important for improving patient satisfaction.
Global trends in research of high-throughput sequencing technology associated with chronic wounds from 2002 to 2022: A bibliometric and visualized study
Authors: Meng Hao , Peng Yu , Li Pinxue , Su Jianlong , Jiang Yufeng , Fu Xiaobing 
Published: February 2023

BackgroundChronic wounds are a complex medical problem. With the difficulty of skin healing, the microbial ecology of chronic wounds is an essential factor affecting wound healing. High-throughput sequencing (HTS) technology is a vital method to reveal the microbiome diversity and population structure of chronic wounds.ObjectiveThe aim of this paper was to delineate the scientific output characteristics, research trends, hotspots and frontiers of HTS technologies related to chronic wounds globally over the past 20 years.MethodsWe searched the Web of Science Core Collection (WoSCC) database for articles published between 2002 and 2022 and their full record information. The Bibliometrix software package was used to analyze bibliometric indicators and VOSviewer visualization analysis results.ResultsUltimately, a total of 449 original articles were reviewed, and the results showed that the number of annual publications (Nps) about HTS associated with chronic wounds has steadily increased over the last 20 years. The United States and China produce the most articles and have the highest H-index, while the United States and England have the largest number of citations (Nc) in this field. The University of California, Wound Repair and Regeneration and National Institutes of Health Nih United States were the most published institutions, journals and fund resources, respectively. The global research could be divided into 3 clusters as follows: microbial infection of chronic wounds, the healing process of wounds and microscopic processes, skin repair mechanism stimulated by antimicrobial peptides and oxidative stress. In recent years, “wound healing”, “infections”, “expression”, “inflammation”, “chronic wounds”, “identification” and “bacteria” “angiogenesis”, “biofilms” and “diabetes” were the most frequently used keywords. In addition, research on “prevalence”, “gene expression”, “inflammation” and “infection” has recently become a hotspot.ConclusionsThis paper compares the research hotspots and directions in this field globally from the perspectives of countries, institutions and authors, analyzes the trend of international cooperation, and reveals the future development direction of the field and research hotspots of great scientific research value. Through this paper, we can further explore the value of HTS technology in chronic wounds to better solve the problem of chronic wounds.
A Na+/H+ Antiporter Gene from Rosa multiflora (RmNHX2) Functions in Salt Tolerance via Modulating ROS Levels and Ion Homeostasis
Authors: Luo Haiyan , Shen Yuxiao , Chen Linmei , Cui Yongyi , Luo Ping 
Published: February 2023

High salinity restricts plant growth and geographic distribution. Plant intracellular Na+/H+ (NHX) antiporters have critical roles in plant development and stress response. However, the molecular functions of RmNHXs in Rosa multiflora remain unclear. In this study, we identified 11 putative RmNHXs in R. multiflora according to the genome-wide analysis. The RmNHXs were classified into three classes. Most of the RmNHXs were responsive to salt stress, with the greatest upregulation being observed in RmNHX2. RmNHX2 was localized at the tonoplast. RmNHX2 overexpression resulted in the enhanced salt tolerance in tobacco, whereas virus-induced gene silencing (VIGS) of RmNHX2 in R. multiflora elevated salt susceptibility. Under salt treatment, the transgenic tobaccos achieved less reactive oxygen species (ROS) accumulation and higher activities of antioxidant enzymes, which complied with the upregulated expressions of antioxidant genes. Moreover, RmNHX2-overexpression lines had a lower level of Na+, a higher level of K+, and a lower Na/K ratio. In contrast to the mentioned, VIGS of RmNHX2 in R. multiflora exhibited the opposite phenotype, accompanied by a compromised salt tolerance. Regarded together, these results demonstrate that RmNHX2 enhances plant salt tolerance by maintaining proper ion homeostasis, as well as by accelerating ROS scavenging.
Spontaneous Reversal of Vitiligo, a Rare Phenomenon Reported in a Case in Saudi Arabia with an Insight into Metabolic Biochemical Derangements
Authors: Alshaikh Ayoub Ali , Bharti Rishi Kumar 
Published: February 2023

Background and Objectives: Vitiligo is a skin disorder characterized by hypopigmented macules occurring due to melanocyte destruction. An interplay of several biochemical mechanisms has been proposed to explain the etiopathogenesis of vitiligo, such as genetic, autoimmune responses, generation of inflammatory mediators, oxidative stress, and melanocyte detachment mechanisms. There is no cure for vitiligo; however, pharmacological treatment measures (cosmetic camouflage creams, steroids, psoralen and ultraviolet A (PUVA) therapy, narrowband UVB) are available, but they could have certain side effects. We reported an interesting case of vitiligo in Saudi Arabia that showed reversal of vitiligo, which is an extremely rare phenomenon, with the objective of probing the probable reasons for this reversal. To the best of our knowledge, there is no study on vitiligo that has reported spontaneous reversal of vitiligo in Saudi Arabia so far. Materials and Method: The patient presented to the Family Medicine clinic with a history of restoration of melanin pigment in his lesions after 3 years of the onset of vitiligo. Patients history was taken carefully along with clinical examination, carried out necessary biomedical lab investigations and compiled the data. The data at the time of pigment restoration were compared to the previous data when he developed the lesions. Result: The probable reasons for vitiligo reversal could be markedly decreased psychological stress, regular consumption of an antioxidant-rich herbal drink made of curcumin and honey, and dietary switchover to vegetarianism and an alcohol-free lifestyle. Conclusions: Curcumin-based herbal remedies could be an alternative option to treat vitiligo. These methods must be further explored through clinical trials as they are safer, easily available, and more affordable.
Adaptation of Proteome and Metabolism in Different Haplotypes of Rhodosporidium toruloides during Cu(I) and Cu(II) Stress
Authors: Cavelius Philipp , Engelhart-Straub Selina , Biewald Alexander , Haack Martina , Awad Dania , Brueck Thomas , Mehlmer Norbert 
Published: February 2023

Rhodosporidium toruloides is a carotenogenic, oleogenic yeast that is able to grow in diverse environments. In this study, the proteomic and metabolic responses to copper stress in the two haplotypes IFO0559 and IFO0880 were assessed. 0.5 mM Cu(I) extended the lag phase of both strains significantly, while only a small effect was observed for Cu(II) treatment. Other carotenogenic yeasts such as Rhodotorula mucilaginosa are known to accumulate high amounts of carotenoids as a response to oxidative stress, posed by excess copper ion activity. However, no significant increase in carotenoid accumulation for both haplotypes of R. toruloides after 144 h of 0.5 mM Cu(I) or Cu(II) stress was observed. Yet, an increase in lipid production was detected, when exposed to Cu(II), additionally, proteins related to fatty acid biosynthesis were detected in increased amounts under stress conditions. Proteomic analysis revealed that besides the activation of the enzymatic oxidative stress response, excess copper affected iron–sulfur and zinc-containing proteins and caused proteomic adaptation indicative of copper ion accumulation in the vacuole, mitochondria, and Golgi apparatus.
Inclusion health patient perspectives on remote access to general practice: a qualitative study
Authors: Verity Aaminah , Tzortziou Brown Victoria 
Published: February 2023

BackgroundThe COVID-19 pandemic has led to rapid and widespread adoption of remote consultations and triage-first pathways in general practice. However, there is a lack of evidence on how these changes have been perceived by patients from inclusion health groups.AimTo explore the perspectives of individuals from inclusion health groups on the provision and accessibility of remote general practice services.Design & settingA qualitative study with individuals from Gypsy, Roma and Traveller communities, sex workers, vulnerable migrants, and those experiencing homelessness, recruited by Healthwatch in east London.MethodThe study materials were co-produced with people with lived experience of social exclusion. Semi-structured interviews with 21 participants were audiorecorded, transcribed, and analysed using the framework method.ResultsAnalysis identified barriers to access owing to lack of translation availability, digital exclusion, and a complex healthcare system, which is difficult to navigate. The role of triage and general practice in emergencies often seemed unclear to participants. Other themes identified included the importance of trust, face-to-face consultation options for ensuring safety, and the benefits of remote access, particularly in terms of convenience and saving time. Themes on reducing barriers included improving staff capacity and communication, offering tailored options and continuity of care, and simplifying care processes.ConclusionThe study highlighted the importance of a tailored approach for addressing the multiple barriers to care for inclusion health groups and the need for clearer and inclusive communication on the available triage and care pathways.
Empresas fantasma
Authors: Requenes José Isabel García , Triana Oralia Salcedo , Romo Daniel Ruiz , González Georgina del Pilar Delijorge , Briones Rosa María Muñoz , Berumen Luis Enrique Paredes , Cornejo Rosa Aída Rivera , Varela Manuel Enrique Villela 
Published: February 2023

Las empresas que realizan operaciones simuladas se han convertido en un grave problema que enfrenta la administración tributaria en México. A este tipo de empresas se les conoce como empresas “fantasma” o factureras. La mayoría de las veces estas empresas no tienen empleados, ni domicilio, pero cuentan con los papeles en regla ante las instancias correspondientes, como registro en Hacienda, ante la Secretaría de Economía, con actas constitutivas, etc., y lo más importante: tienen los sellos para emitir facturas por dar servicios o producir bienes que son incapaces de proveer. Son empresas que no existen físicamente y que por lo general se utilizan para cometer presuntos delitos como la evasión de impuestos, lavado de dinero o el desvío de recursos públicos. Las empresas “fantasma” por sí mismas no evaden impuestos, ni lavan dinero, ni desaparecen recursos públicos, sino que requieren de toda una estructura de funcionarios corruptos que diseñen el esquema para simular contrataciones para desviar el dinero, así como de múltiples operadores, como asesores financieros, abogados, notarios, despachos de contadores y prestanombres que lo ejecuten al más puro estilo del crimen organizado, además del contubernio de unas autoridades que permitan y favorezcan la impunidad y la protección de esta red de corrupción. En la administración pública de México se han utilizado para disfrazar el desvío de recursos, que deberían servir para el beneficio de los mexicanos, en favor de un particular. En siete años, el Servicio de Administración Tributaria (SAT) ha detectado casi 11,000 empresas fantasma. La última actualización de la lista, al 16 de diciembre del 2021, expone que se identificaron un total de 10,990 empresas que facturan operaciones simuladas (EFOS) por más de dos billones de pesos. El problema es tan grave que el sector público adquirió entre el 50 y 70% del total de las facturas falsas detectadas en años recientes.