bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leaf Microbiome Data for European Cultivated Grapevine (Vitis vinifera) During Downy Mildew (Plasmopara viticola) Epidemics in Three Wine-Producing Regions in France
Authors: Barroso-Bergadà Didac , Delmotte François , Faivre d'Arcier Julie , Massot Marie , Chancerel Emilie , Demeaux Isabelle , Guimier Soizic , Guichoux Erwan , Bohan David A. , Vacher Corinne 
Published: March 2023

Grapevine downy mildew ( Plasmopara viticola) is a major disease of European cultivated grapevine ( Vitis vinifera L.) against which a large amount of synthetic pesticides are used. Developing microbial biocontrol of P. viticola could reduce the use of pesticides in viticulture and preserve human and environmental health. To achieve this goal, the ecological interactions that develop during infection between P. viticola and the vine foliar microbiome need to be explored. Here, we present metabarcoding datasets describing the bacterial and fungal communities of pairs of symptomatic and asymptomatic leaf samples collected during downy mildew epidemics in three major wine-producing regions of France. Fungal and bacterial communities were sequenced on a MiSeq Illumina platform, and the abundance of the oomycete P. viticola was quantified using qPCR. We provide the raw metabarcoding datasets, the amplicon sequence variant tables obtained after bioinformatic processing, the metadata describing sampling sites and tissue health conditions, and the code used for bioinformatic analysis. These datasets will enable microbiome comparison within pairs of symptomatic and asymptomatic samples collected at the same time on the same leaf. Such a comparison could help describe the ecological interactions between P. viticola and the grapevine foliar microbiome. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Assessment of the antidiabetic potential of extract and novel phytoniosomes formulation ofTradescantia pallidaleaves in the alloxan‐induced diabetic mouse model
Authors: Imtiaz Fariha , Islam Muhammad , Saeed Hamid , Ahmed Abrar , Rathore Hassaan Anwer 
Published: March 2023

AbstractDiabetes inflicts health and economic burdens on communities and the present antidiabetic therapies have several drawbacks.Tradescantia pallidaleaves have been used as a food colorant and food preservative; however, to our knowledge antidiabetic potential of the leaves ofT. pallidahas not been explored yet. The current study aimed to investigate the antidiabetic potential ofT. pallidaleaves extract and its comparison with the novel nisosome formulation of the extract. The leaves extract and phytoniosomes ofT. pallidain doses of 15, 25 and 50 mg/kg were used to assess the oral glucose loaded, and alloxan‐induced diabetic mice models. The biological parameters evaluated were; change in body weight, blood biochemistry, relative organ to body weight ratio and histopathology of the liver, pancreas and kidney. Results revealed that the extract 50 mg/kg and phytoniosomes 25 and 50 mg/kg remarkably reduced the blood glucose level in all hyperglycemic mice by possibly inhibiting α‐amylase and α‐glucosidase production. Body weight and blood biochemical parameters were considerably improved in phytoniosomes 50 mg/kg treated group. The relative body weight was similar to those of healthy mice in extract 50 mg/kg, phytoniosomes 25 mg/kg, and phytoniosomes 50 mg/kg treated groups. Histopathology showed the regeneration of cells in the CHN50 treated group. Hyphenated chromatographic analysis revealed potent metabolites, which confirmed the antidiabetic potential of the extract by inhibiting α‐amylase and α‐glucosidase using in silico analysis. The present data suggested that phytoniosomes have shown better antidiabetic potential than crude extract of these leaves.
Randomized Pilot Test of a Decision Support Tool for Acute Appendicitis
Authors: Rosen Joshua E. , Flum David R. , Davidson Giana H. , Liao Joshua M. 
Published: March 2023

Objective:
To test the effect of a new decision support tool for acute appendicitis and assess its efficacy and acceptability.

Background:
Mounting evidence from randomized controlled trials have shown that antibiotics can be a safe and effective treatment for appendicitis. Patients and surgeons must work together to choose the optimal treatment approach for each patient based on their own preferences and values. We developed a decision support tool to facilitate shared decision-making for appendicitis and its effect on decisional outcomes remains unknown.

Methods:
We conducted an online randomized field test in at-risk individuals comparing the decision support tool to a standard infographic. Individuals were randomized 3:1 to view the decision support tool or infographic. The primary outcome was the total decisional conflict scale (DCS) score measured before and after exposure to the decision support tool. Secondary outcomes included between-group DCS scores, and between-group comparisons of the acceptability.

Results:
One hundred eighty individuals were included in the study. Total DCS scores decreased significantly after viewing the decision support tool (59 [95% confidence interval (CI): 55–63] to 15 [95% CI: 12–17], P < 0.001) representing movement from a state of high to low decisional conflict. Individuals exposed to the decision support tool reported higher acceptability ratings (3.7 [95% CI: 3.6–3.8] vs 3.3 [95% CI: 3.2–3.5] out of 4) and demonstrated increased willingness to consider both treatment options.

Conclusions:
These data support the further use and testing of this novel decision support tool in patients with acute appendicitis.
Patient Perceptions of Decision-making and Quality-of-life Following Surgical Resection of Pancreatic Adenocarcinoma
Authors: Bleicher Josh , Place Aubrey , Harris Alex H. S. , Scaife Courtney L. , Huang Lyen C. 
Published: March 2023

Background:
Pancreatic adenocarcinoma (PDAC) remains a highly lethal disease, with surgery, the only opportunity for cure, accompanied by high rates of morbidity. Understanding patients’ lived experiences after surgical resection of PDAC is critical to knowing whether the decision to undergo surgery was worth it for these patients.

Methods:
We performed a convergent, mixed-methods study with patients who underwent resection of PDAC between January 1, 2019, and January 8, 2020. Quantitative data (medical record review and 3 questionnaires) were analyzed using descriptive statistics. Qualitative data (semistructured interviews) were analyzed using the constant comparative method. Data were then compared for congruence.

Results:
Eighteen of 22 eligible participants completed interviews and 11 completed questionnaires. Data collection occurred at a median of 14.2 months (IQR 11.6–16.3) from surgery. We identified 4 main themes. First, persistent negative symptoms were common for patients, but patients adapt to these and are satisfied with their “new normal.” Second, patients have varied and continually evolving mindsets throughout their cancer journey. Third, despite decreased quality-of-life, patients have a high degree of satisfaction with their decision to pursue surgery. Finally, patients were okay with a passive role in decision-making around surgery. Despite variable involvement in decision-making and outcomes, no participants reported regret over the decision to pursue surgery.

Discussion:
This nuanced account of patients’ lived experiences following surgery for PDAC allows for an improved understanding of the impact of pancreatic resection on patients. Surgeons can use these data to improve preoperative counseling for patients with PDAC and help guide them to making the correct decisions about surgery.
Long-Term Outcomes of Venous Resections in Pancreatic Ductal Adenocarcinoma Patients
Authors: Sillesen Martin , Hansen Carsten Palnæs , Dencker Emilie Even , Burgdorf Stefan Kobbelgaard , Krohn Paul Suno , Stender Mogens Tornby , Fristrup Claus Wilki , Storkholm Jan Henrik 
Published: March 2023

Objective:
To investigate whether pancreatic resections (PR) for pancreatic ductal adenocarcinoma (PDAC) is associated with worse survival when resection of the superior mesenteric vein/portal vein (SMV/PV) is required.

Background:
PR for PDAC with resection of the superior mesenteric vein/portal vein (SMV/PV, PR+V resection) may be associated with inferior overall survival (OS) compared with PR without the need for SMV/PV resection (PR–V). We hypothesized that PR+V results in lower OS compared with PR–V.

Method:
Retrospective study using data from the nationwide Danish Pancreatic Cancer Database from 2011 to 2020. Data on patients who underwent PR for PDAC were extracted. A group of PR patients found nonresectable on exploratory laparotomy (EXP) was also included. OS was assessed using Kaplan-Meier and Cox proportional hazards models adjusting for confounders (age, sex, R-resection level, chemotherapy, comorbidities, histology T and N classification, procedure subtype as well as tumor distance to the SMV/PV).

Results:
Overall, 2403 patients were identified. Six hundred two underwent exploration only (EXP group), whereas 412 underwent pancreatic resection with (PR+V group) and 1389 (PR–V) without SMV/PV resection. Five-year OS for the PR+V group was lower (20% vs 30%) compared with PR–V, although multivariate Cox proportional hazards modeling could not associate PR+V status with OS (Hazard ratio 1.11, P = 0.408).

Conclusion:
When correcting for confounders, PR+V was not associated with lower OS compared with PR–V.
Robotic Magnetic Surgery: Results From the First Prospective Clinical Trial
Authors: Romero-Velez Gustavo , Robles Ignacio , Jiménez Julio , Cabrera Carolina , Luengas Rafael , Portenier Dana , Kroh Matthew 
Published: March 2023

Objectives:
To evaluate a novel Magnetic Robotic Platform during reduced-port laparoscopic surgery in a prospective, multicenter clinical trial.

Background:
Magnetic surgery has been developed to increase the benefits of minimally invasive surgery, with prior studies demonstrating its clinical benefits. Robotic-assisted surgery increases the surgeon’s control over the instruments, offering less dependency on an assistant. The synergism of both techniques may escalate these individual benefits.

Methods:
A prospective, multicenter, single-arm, open-label study was performed to assess the safety and performance of a robotic magnetic surgical system (Levita Robotic Platform). The investigational device was used during reduced-port laparoscopic cholecystectomy and laparoscopic bariatric surgery. The primary endpoints evaluated were safety and feasibility. Patients were followed for 30 days post-procedure.

Results:
Between May 2021 and December 2021, 30 patients undergoing laparoscopic surgery were recruited. There were 22 females and 8 males with a mean age of 39 years (22–69 years) and median body mass index of 33 kg/m2 (21.6–50.4 kg/m2). Procedures included 15 gastric sleeves, 14 cholecystectomies, and 1 Roux en-Y gastric bypass. The procedures were successfully performed by utilizing the robotic magnetic surgical system and a reduced-port technique in all 30 patients. No device-related serious adverse events were reported. The device provided adequate visualization and retraction in all cases.

Conclusions:
This clinical trial shows for the first time that this novel Magnetic Robotic Platform is safe and feasible in reduced-port laparoscopic cholecystectomy and laparoscopic bariatric surgery. This robotic platform brings the benefits of magnetic surgery in terms of reduction of incisions plus increasing the control for the surgeon.
Association of Hospital Bed Turnover With Patient Outcomes in Digestive Surgery
Authors: Pasquer Arnaud , Pascal Léa , Polazzi Stephanie , Skinner Sarah , Poncet Gilles , Lifante Jean-Christophe , Duclos Antoine 
Published: March 2023

Objective:
To determine the influence of hospital bed turnover rate (BTR) on the occurrence of complications following minor or major digestive surgery.

Background:
Performance improvement in surgery aims at increasing productivity while preventing complications. It is unknown whether this relationship can be influenced by the complexity of surgery.

Methods:
A nationwide retrospective cohort study was conducted, based on generalized estimating equation modeling to determine the effect of hospital BTR on surgical outcomes, adjusting for patient mix and clustering within 631 public and private French hospitals. All patients who underwent minor or major digestive surgery between January 1, 2013 and December 31, 2018 were included. Hospital BTR was defined as the annual number of stays per bed for digestive surgery and categorized into tertiles. The primary endpoint was a composite measurement of events occurring within 30 days after surgery: inpatient death, extended intensive care unit (ICU) admission, and reoperation.

Results:
Rate of adverse events was 2.51% in low BTR hospitals versus 2.25% in high BTR hospitals for minor surgery, and 16.79% versus 16.83% for major surgery. Patients who underwent minor surgery in high BTR hospitals experienced lower complications (odds ratio [OR], 0.89; 95% confidence interval [CI], 0.81–0.97; P = 0.009), mortality (OR, 0.87; 95% CI, 0.78–0.98, P = 0.02), ICU admission (OR, 0.83; 95% CI, 0.70–0.99; P = 0.03), and reoperation (OR, 0.91; 95% CI, 0.85–0.97; P = 0.002) compared to those in low BTR hospitals. Such differences were not consistently observed among patients admitted for major surgery.

Conclusions:
High turnover of patients in beds is beneficial for minor procedures, but questionable for major surgeries.
Long-term Outcomes and Factors Predicting Outcome of Ileal Pouch-Anal Anastomosis When Used Intentionally for Well-Defined Crohn’s Disease
Authors: Aviran Eyal , Assaf Dan , Zaghiyan Karen , Fleshner Phillip 
Published: March 2023

BACKGROUND:
Crohn’s disease (CD) is considered a contraindication for ileal pouch-anal anastomosis (IPAA). Our prior study of IPAA when used intentionally for well-defined CD showed a high incidence of recurrent disease with a low incidence of pouch failure

OBJECTIVE:
Replicate these findings in a larger cohort over a longer period

DESIGN:
Retrospective review of a prospective inflammatory bowel disease registry

SETTINGS:
Large IBD referral center

PATIENTS:
Preoperative colorectal CD requiring surgery

INTERVENTION:
Ileal pouch-anal anastomosis

MAIN OUTCOME:
Long-term CD recurrence, pouch failure and pouch function

RESULTS:
Forty-six patients were identified. CD was diagnosed based on perianal disease (n = 18; 39%), small bowel disease (n = 16; 35%), noncaseating granuloma (n = 10; 22%) and discontinuous inflammation (colorectal skip lesions) (n = 11; 24%). After a median follow up of 93 (7-291) months, 22 (48%) patients developed recurrent CD based on afferent limb disease (n = 14; 30%) or pouch fistulizing disease (n = 8; 18%). Only 4 (9%) patients developed pouch failure. No clinical factor was associated with CD recurrence. Young age at the time of surgery and short duration of disease prior to IPAA were associated with pouch fistula recurrence (p = 0.003 and p = 0.03, respectively). Higher preoperative platelet count was the only clinical characteristic significantly associated with pouch failure (p = 0.02). Most patients (86%) reported excellent continence, with no urgency (67%) and median stool frequency of 6 (range, 3-9) per day.

LIMITATION:
Retrospective nature and relatively small sample size.

CONCLUSION:
This largest reported series examining the intentional use of IPAA in CD showed a high (48%) incidence of postoperative CD with a low (9%) incidence of pouch failure. Young age and short disease course prior to surgery were risk factors for poor outcome. Highly motivated patients with colorectal CD may consider an IPAA and avoid a definitive ileostomy.
Assessing Rates of Positive Surgical Margins After Standard Excision of Vulvar Melanomas
Authors: Cheraghlou Shayan , Doudican Nicole A. , Criscito Maressa C , Stevenson Mary L. , Carucci John A. 
Published: March 2023

BACKGROUND
Vulvar melanoma is a rare malignancy with frequent recurrence and poor prognosis. National guidelines recommend wide local excision of these tumors with allowances for narrower margins for anatomic and functional limitations, which are common on specialty sites. There is presently a lack of data of margin positivity after standard excision of vulvar melanomas.

OBJECTIVE
We aim to evaluate the rate of positive margins after standard excision of vulvar melanomas.

MATERIALS AND METHODS
Retrospective cohort study of surgically excised vulvar melanomas from the NCDB diagnosed from 2004 to 2019.

RESULTS
We identified a total of 2,226 cases. Across surgical approaches and tumor stages, 17.2% (Standard Error [SE]: 0.8%) of cases had positive surgical margins. Among tumor stages, T4 tumors were most commonly excised with positive margins (22.9%, SE: 1.5%). On multivariable survival analysis, excision with positive margins was associated with significantly poorer survival (Hazard Ratio 1.299, p = .015).

CONCLUSION
We find that positive margin rates after standard excision of vulvar malignancies are higher than for other specialty site melanomas. Our data suggest that use of surgical approaches with complete margin assessment may improve local control and functional outcomes for patients with vulvar melanoma as they have for patients with other specialty site melanomas.
PhD-Prepared Nurses' Areas of Inquiry
Authors: Roberts Lisa R. , D'Errico Ellen 
Published: March 2023

Background:
Nursing science is heavily dependent on nurse scientists with adequate funding. Nurse scientists should be aware of organizations' research agendas and, to the extent possible, align research with stated research priorities. Current information on the demographics of nurse scientists, their projects, and the alignment to known priorities is illusive.

Purpose:
To describe areas of inquiry pursued by PhD-prepared nurses and congruence with the research agendas of nursing organizations.

Methods:
A descriptive, exploratory approach was undertaken with PhD-prepared nurse scientists to gather data about their PhD program, dissertation topic, current work status, productivity, and future recommendations.

Results:
Key findings were that dissertation topics were broad and aligned with research priorities. Elements influencing research productivity are described.

Conclusions:
Understanding current nursing science stewardship and intentionally strategizing for the future of research, academia, and clinical practice will enhance our ability to tailor PhD programs accordingly.
Case Report: Multimodal, Longitudinal Assessment of Retinal Structure and Function following Laser Retinal Injury
Authors: Wang Yiyi , La Tammy T. , Mason Melanie , Tuten William S. , Roorda Austin 
Published: March 2023

SIGNIFICANCE
This case report demonstrates the use of novel imaging techniques and functional tests to longitudinally evaluate retinal structure and function after laser retinal injury. The structural and functional prognosis could be predicted with clinical findings, high-resolution retinal imaging, and functional testing.

PURPOSE
We present a laser retinal injury case in which an adaptive optics scanning laser ophthalmoscope and adaptive optics–based psychophysics were used to examine and monitor retinal structure and function after accidental exposure to a 1-W infrared laser beam.

CASE REPORT
A 23-year-old patient was unwittingly exposed to a 1-W, 852-nm continuous-wave laser at work as they noticed a small central blurry spot in the right eye. An initial eye examination was done 1 day after exposure, and the right eye's acuity was 20/25−2. Posterior segment evaluation revealed disrupted outer retina near the right eye's fovea. Adaptive optics imaging 2 weeks after the exposure revealed a 0.50 × 0.75° elliptical area with irregular borders and abnormal cone reflectivity just below the fovea. Starting at 1-month follow-up, structural recovery was observed on optical coherence tomography (OCT). Subsequent adaptive optics imaging showed significant recovery of cone reflectivity. Importantly, adaptive optics microperimetry showed measurable detection thresholds at all affected retinal locations at 6 months. By 10 months, all sites exhibited normal sensitivities.

CONCLUSIONS
Retinal structure and function from laser injury can be visualized and measured with OCT, adaptive optics imaging, and psychophysics. An intact Bruch's membrane on OCT and measurable retinal sensitivity by adaptive optics microperimetry may serve as good biomarkers for retinal recovery.
Eliminating Breast Surgery for Invasive Cancer with Exceptional Response to Neoadjuvant Systemic Therapy: Prospective Multicenter Clinical Trial Planned Initial Feasibility Endpoint
Authors: Johnson Helen M , Valero Vicente , Yang Wei T , Smith Benjamin D , Krishnamurthy Savitri , Shen Yu , Lin Heather , Lucci Anthony , Rauch Gaiane M , Kuerer Henry M 
Published: March 2023

Background:
Response to neoadjuvant systemic therapy (NST) for breast cancer enables tailoring of subsequent therapies. Image-guided breast biopsy after NST can accurately predict a pathologic complete response (pCR). The feasibility phase of the clinical trial reported here assesses omission of breast surgery followed by radiotherapy in terms of local recurrence prior to trial expansion.

Study Design:
Women with unicentric, cT1-2 N0-1 M0 triple-negative (TNBC) or HER2-positive (HER2+BC) cancer with <2cm residual disease on post-NST imaging were eligible to enroll. If no residual invasive or in-situ disease was identified by image-guided, vacuum-assisted core biopsy (VACB), breast surgery was omitted, and radiotherapy delivered. The primary endpoint for the feasibility phase was ipsilateral breast tumor recurrence (IBTR) at 6 months. If any recurrence occurred during the feasibility phase the trial would halt.

Results:
Thirteen patients were enrolled from March 2017 to October 2018. The mean age was 60.8 years (range 51-75) and most patients were white (69.2%), non-Hispanic/Latino (84.6%). All patients had invasive ductal carcinoma (6 TNBC, 7 HER2+B). Mean tumor size was 2.4 centimeters (range 0.9-5.0 cm) before NST and 0.7cm (range 0-1.8cm) post-NST. Seven patients (53.8%) had residual disease identified on VACB; the remaining six (46.2%) comprised the feasibility cohort. At a median follow-up of 44.3 (range 41.3-51.3) months, there were no IBTR in this cohort.

Conclusions:
These early data suggest that omission of breast surgery in patients with invasive TNBC and HER2+BC with no evidence of residual disease on standardized VACB following NST is potentially feasible. Results from the expansion phase of this clinical trial will be reported per protocol pre-specified analyses.