bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of a copper-related gene prognostic signature in hepatocellular carcinoma
Authors: Shi Haoting , Huang Jingxuan , Wang Xue , Li Runchuan , Shen Yiqing , Jiang Bowen , Ran Jinjun , Cai Rong , Guo Fang , Wang Yufei , Ren Gang 
Published: July 2023

Introduction: Reliable biomarkers are in need to predict the prognosis of hepatocellular carcinoma (HCC). Whilst recent evidence has established the critical role of copper homeostasis in tumor growth and progression, no previous studies have dealt with the copper-related genes (CRGs) signature with prognostic potential in HCC.Methods: To develop and validate a CRGs prognostic signature for HCC, we retrospectively included 353 and 142 patients as the development and validation cohort, respectively. Copper-related Prognostic Signature (Copper-PSHC) was developed using differentially expressed CRGs with prognostic value. The hazard ratio (HR) and the area under the time-dependent receiver operating characteristic curve (AUC) during 3-year follow-up were utilized to evaluate the performance. Additionally, the Copper-PSHC was combined with age, sex, and cancer stage to construct a Copper-clinical-related Prognostic Signature (Copper-CPSHC), by multivariate Cox regression. We further explored the underlying mechanism of Copper-PSHC by analyzing the somatic mutation, functional enrichment, and tumor microenvironment. Potential drugs for the high-risk group were screened.Results: The Copper-PSHC was constructed with nine CRGs. Patients in the high-risk group demonstrated a significantly reduced overall survival (OS) (adjusted HR, 2.65 [95% CI, 1.83–3.84] and 3.30, [95% CI, 1.27–8.60] in the development and validation cohort, respectively). The Copper-PSHC achieved a 3-year AUC of 0.74 [95% CI, 0.67–0.82] and 0.71 [95% CI, 0.56–0.86] for OS in the development and validation cohort, respectively. Copper-CPSHC yield a 3-year AUC of 0.73 [95% CI, 0.66–0.80] and 0.72 [95% CI, 0.56–0.87] for OS in the development and validation cohort, respectively. Higher tumor mutation burden, downregulated metabolic processes, hypoxia status and infiltrated stroma cells were found for the high-risk group. Six small molecular drugs were screened for the treatment of the high-risk group.Conclusion: Copper-PSHC services as a promising tool to identify HCC with poor prognosis and to improve disease outcomes by providing potential clinical decision support in treatment.
Predictive value of the Framingham steatosis index for cardiovascular risk: a nationwide population-based cohort study
Authors: Cho Yun Kyung , Kim Myungjin , Kim Ye-Jee , Jung Chang Hee , Lee Woo Je , Park Joong-Yeol 
Published: July 2023

BackgroundNon-alcoholic fatty liver disease (NAFLD) is common and is associated with cardiovascular (CV) disease and mortality. The Framingham steatosis index (FSI) was recently proposed as a diagnostic marker of NAFLD and was calculated from age, body mass index, triglyceride, aspartate aminotransferase, alanine aminotransferase, diabetes history, and hypertension status. We aimed to evaluate the predictive ability of FSI for CV risk using a large-scale population dataset from the Korean National Health Insurance Service–National Health Screening Cohort (NHIS–HEALS).MethodsAmong 514,866 individuals in the NHIS–HEALS, we excluded those who died, had a history of admission due to a CV event, and were heavy drinkers. The final study cohort comprised 283,427 participants. We employed both unadjusted and covariate-adjusted models in Cox proportional hazards regression analyses to determine the association between FSI and major adverse cardiovascular events (MACEs), CV events, and CV mortality.ResultsDuring a median follow-up of 5.9 years, we documented 9,674, 8,798, and 1,602 cases of MACEs, CV events, and CV mortality, respectively. The incidence of MACEs was 1.28%, 2.99%, 3.94%, and 4.82% in the first to fourth quartiles of FSI, respectively. The adjusted hazard ratios (95% confidence interval) for MACEs gradually and significantly increased with the FSI quartiles [1.302 (1.215–1.395) in Q2, 1.487 (1.390–1.590) in Q3, and 1.792 (1.680–1.911) in Q4], following an adjustment for conventional CV risk factors, including age, sex, smoking, drinking, physical activities, low-density lipoprotein cholesterol, estimated glomerular filtration rate, and waist circumference. Participants in the higher quartiles of FSI exhibited a noteworthy increase in the occurrence of CV event. However, upon adjusting for relevant risk factors, the association between FSI and CV mortality did not reach statistical significance.ConclusionOur study suggests that the FSI, which is a surrogate marker of NAFLD, has a prognostic value for detecting individuals at higher risk of CV events.
Comparison of joint status using ultrasound assessments and Haemophilia Joint Health Score 2.1 in children with haemophilia
Authors: Li Yanju , Wang Feiqing , Pan Chengyun , Zhang Jing , Zhang Qian , Ban Lingying , Song Lingling , Wang Jishi , He Zhixu , Zeng Xiaojing , Tang Dongxin , Liu Yang 
Published: July 2023

IntroductionUltrasound (US) has gained popularity in the evaluation of haemophilic joint diseases because it enables the imaging of soft-tissue lesions in the joints and bone-cartilage lesions. We aimed to determine the correlation between US evaluations and clinical assessments performed using HJHS 2.1 and to evaluate their respective characteristics in assessing early haemophilic arthropathy.MethodsA total of 178 joints (32 knees, 85 elbows, and 61 ankles) in 45 haemophilia A patients (median age, 10 years; range, 6–15) were assessed using US and HJHS 2.1. Ultrasonographic scoring was performed in consensus assessments by one imager by using the US scores.ResultsThe total HJHS 2.1 and US scores showed a strong correlation (rS=0.651, P=0.000, CI: 0.553–0.763), with an excellent correlation for the elbows (rS=0.867, P=0.000, CI: 0.709–0.941) and a substantial correlation for the knees (rS=0.681, P=0.000, CI: 0.527–0.797). The correlation for the ankles was relatively moderate (rS=0.518, P=0.000, CI: 0.308–0.705). Nine subjects (15.5%) without abnormalities, as indicated by HJHS 2.1, showed haemophilic arthropathy in US scoring. All nine joints showed moderate (1/9) to severe (8/9) synovial thickening in the ankle (5/9) and elbow joints (4/9). In contrast, 50 joints (50.5%) showed normal US scores and abnormal changes as indicated by HJHS 2.1. S scores correlated well with HJHS 2.1 for overall and individual joints.DiscussionUS could identify some early pathological changes in joints showing normal clinical findings, but still cannot replace the HJHS; however, it can serve as an imaging examination complementing HJHS 2.
Field testing and psychometric properties of Thai version of the Boston carpal tunnel questionnaire
Authors: Atthakomol Pichitchai , Nudchapong Jirawat , Sangseekaew Kamonwan , Manosroi Worapaka , Tongprasert Siam , Wongpakaran Tinakon , Wongpakaran Nahathai 
Published: July 2023

IntroductionThe Boston Carpal Tunnel Questionnaire (BCTQ) is a widely recommended patient-reported outcome measure to evaluate symptoms and functions in carpal tunnel syndrome (CTS) patients. We aimed to evaluate the translation and cross-cultural adaptation of the Thai version of the BCTQ (Thai BCTQ) and to investigate the psychometric properties including internal consistency, test-retest reliability, construct validity and responsiveness.MethodsThe Thai BCTQ was field tested with 15 healthy volunteers and 15 CTS patients to evaluate the item-objective congruence of each item. Following that, one hundred and twenty-four CTS patients were included for psychometric evaluation in this study. Internal consistency was assessed using Cronbach’s alpha. Test-retest reliability was examined using the intraclass correlation coefficient (ICC). To evaluate construct validity, Spearman’s rank correlation of the symptom severity scale (Thai BCTQ -S), the functional status scale (Thai BCTQ -F) and the subscales of the Thai MHQ were analyzed. Responsiveness was determined using the standardized response mean (SRM).ResultsMinor modification of the Thai version was made to better explain the term “tingling”. The Thai BCTQ-S, Thai BCTQ-F and Thai BCTQ demonstrated adequate Cronbach’s alpha values (0.91-0.94) and good test-retest reliability (ICC=0.89-0.98). Regarding related dimensions, a strong correlation (r=0.67, P&lt;0.008) was found between the Thai BCTQ-F and the Function subscale of Thai MHQ as well as between Thai BCTQ-F and the Activities of Daily Living subscale of the Thai MHQ (r=0.75, P&lt;0.008). In unrelated dimensions, there was a relatively weak correlation between the Thai BCTQ-S and the Aesthetics subscale of the Thai MHQ (r=0.32, P=0.0116). The SRM of the Thai BCTQ was 1.46, indicating large responsiveness.DiscussionThe Thai BCTQ has adequate internal consistency in both the symptom and function scales as well as good construct validity and test-retest reliability indicating it is suitable for evaluating Thai CTS patients. This tool also has a high ability to detect clinically significant changes in symptoms and function over time after receiving conservative or surgical treatment.
Metabolomic and transcriptomice analyses of flavonoid biosynthesis in apricot fruits
Authors: Chen Yilin , Li Wenwen , Jia Kai , Liao Kang , Liu Liqiang , Fan Guoquan , Zhang Shikui , Wang Yatong 
Published: July 2023

IntroductionFlavonoids, as secondary metabolites in plants, play important roles in many biological processes and responses to environmental factors.MethodsApricot fruits are rich in flavonoid compounds, and in this study, we performed a combined metabolomic and transcriptomic analysis of orange flesh (JN) and white flesh (ZS) apricot fruits.Results and discussionA total of 222 differentially accumulated flavonoids (DAFs) and 15855 differentially expressed genes (DEGs) involved in flavonoid biosynthesis were identified. The biosynthesis of flavonoids in apricot fruit may be regulated by 17 enzyme-encoding genes, namely PAL (2), 4CL (9), C4H (1), HCT (15), C3’H (4), CHS (2), CHI (3), F3H (1), F3’H (CYP75B1) (2), F3’5’H (4), DFR (4), LAR (1), FLS (3), ANS (9), ANR (2), UGT79B1 (6) and CYP81E (2). A structural gene-transcription factor (TF) correlation analysis yielded 3 TFs (2 bHLH, 1 MYB) highly correlated with 2 structural genes. In addition, we obtained 26 candidate genes involved in the biosynthesis of 8 differentially accumulated flavonoids metabolites in ZS by weighted gene coexpression network analysis. The candidate genes and transcription factors identified in this study will provide a highly valuable molecular basis for the in-depth study of flavonoid biosynthesis in apricot fruits.
Comprehensive meta-analysis of QTL and gene expression studies identify candidate genes associated with Aspergillus flavus resistance in maize
Authors: Baisakh Niranjan , Da Silva Eduardo A. , Pradhan Anjan K. , Rajasekaran Kanniah 
Published: July 2023

Aflatoxin (AF) contamination, caused by Aspergillus flavus, compromises the food safety and marketability of commodities, such as maize, cotton, peanuts, and tree nuts. Multigenic inheritance of AF resistance impedes conventional introgression of resistance traits into high-yielding commercial maize varieties. Several AF resistance-associated quantitative trait loci (QTLs) and markers have been reported from multiple biparental mapping and genome-wide association studies (GWAS) in maize. However, QTLs with large confidence intervals (CI) explaining inconsistent phenotypic variance limit their use in marker-assisted selection. Meta-analysis of published QTLs can identify significant meta-QTLs (MQTLs) with a narrower CI for reliable identification of genes and linked markers for AF resistance. Using 276 out of 356 reported QTLs controlling resistance to A. flavus infection and AF contamination in maize, we identified 58 MQTLs on all 10 chromosomes with a 66.5% reduction in the average CI. Similarly, a meta-analysis of maize genes differentially expressed in response to (a)biotic stresses from the to-date published literature identified 591 genes putatively responding to only A. flavus infection, of which 14 were significantly differentially expressed (−1.0 ≤ Log2Fc ≥ 1.0; p ≤ 0.05). Eight MQTLs were validated by their colocalization with 14 A. flavus resistance-associated SNPs identified from GWAS in maize. A total of 15 genes were physically close between the MQTL intervals and SNPs. Assessment of 12 MQTL-linked SSR markers identified three markers that could discriminate 14 and eight cultivars with resistance and susceptible responses, respectively. A comprehensive meta-analysis of QTLs and differentially expressed genes led to the identification of genes and makers for their potential application in marker-assisted breeding of A. flavus-resistant maize varieties.
Sistema de presión negativa en loxoscelismo cutáneo-visceral. Reporte de un caso
Authors: Rivera  Pérez Miguel Angel , Villalobos López Jesús Antonio , Valle Leal Jaime Guadalupe 
Published: July 2023

Introducción: Las mordeduras por araña parda pueden manifestarse desde una simple lesión dérmica en el área de la mordedura, hasta formas graves, con falla orgánica múltiple.Caso clínico: presentamos el caso de una paciente con mordedura por araña parda, quien presenta inicialmente lesiones dérmicas sin necrosis, evolucionando con áreas de necrosis y el desarrollo de síndrome compartimental de extremidad, sepsis, choque séptico y falla renal. Mejora tras manejo intensivo, anti veneno y colocación de terapia de presión negativa (TPN) en herida, conservando la extremidad afectada integra y recuperando la función renal.Conclusiones: Este caso en particular presenta los tres tipos de manifestaciones que se generan en el loxoscelismo, las cuales son una forma de presentación poco frecuente. El tratamiento con TPN se ha introducido como una terapia poderosa, no farmacológica para ayudar a acelerar el proceso de cicatrización de heridas y puede ser de utilidad en pacientes con mordedura de araña (loxoscelismo).
Early Hard X-Rays from the Nearby Core-collapse Supernova SN 2023ixf
Authors: Grefenstette Brian W. , Brightman Murray , Earnshaw Hannah P. , Harrison Fiona A. , Margutti R. 
Published: July 2023

Abstract
We present NuSTAR observations of the nearby SN 2023ixf in M101 (d = 6.9 Mpc) that provide the earliest hard X-ray detection of a nonrelativistic stellar explosion to date at δ
t ≈ 4 days and δ
t ≈ 11 days. The spectra are well described by a hot thermal bremsstrahlung continuum with T > 25 keV shining through a thick neutral medium with a neutral hydrogen column that decreases with time (initial N
Hint = 2.6 × 1023 cm−2). A prominent neutral Fe Kα emission line is clearly detected, similar to other strongly interacting supernovae (SNe) such as SN 2010jl. The rapidly decreasing intrinsic absorption with time suggests the presence of a dense but confined circumstellar medium (CSM). The absorbed broadband X-ray luminosity (0.3–79 keV) is L

X
≈ 2.5 × 1040 erg s−1 during both epochs, with the increase in overall X-ray flux related to the decrease in the absorbing column. Interpreting these observations in the context of thermal bremsstrahlung radiation originating from the interaction of the SN shock with a dense medium we infer large particle densities in excess of n
CSM ≈ 4 × 108 cm−3 at r < 1015 cm, corresponding to an enhanced progenitor mass-loss rate of

M

̇

≈
3
×

10

−
4

M
⊙ yr−1 for an assumed wind velocity of v

w
= 50 km s−1.
Work-life balance among mental health professionals in a tertiary care neuropsychiatry centre in India
Authors: Savarimalai Rajamanikandan , Christy Jayakumar , Bhaskarapillai Binukumar , Damodharan Dinakaran , Sekar Kasi 
Published: July 2023

ABSTRACT

Background:
Stress and distress are common among Mental Health Professionals (MHP). MHPs avoid seeking professional help to address their stress, leading to increased vulnerability to stress and mental health conditions. Inadequately addressed stress directly impacts their service delivery and well-being. However, a serious dearth of literature from India has examined the extent of work-life balance (WLB) stress and psychological distress (PD) among MHP.

Aim:
This cross-sectional study aims to assess WLB and examine the relationship between perceived stress (PS), PD, burnout, and WLB among MHP in a tertiary care neuropsychiatry center.

Methods:
Using stratified random sampling, 101 MHP were evaluated using Pareek’s work-life balance scale, Stamm’s professional quality of life (ProQoL) scale, WHO’s self-reporting questionnaire, and Cohen’s perceived stress scale. Data were analyzed using appropriate parametric or non-parametric tests.

Results:
The overall mean score on WLB was 80.45 (SD = 10.21), the PS scale was 24.86 (SD = 6.87), the burnout score was 22.68 (SD = 6), the ST score was 21.18 (SD = 6.35), and PD score was 5.07 (SD = 5.23). The level of WLB score was average to below average among 64% of participants. There is a positive correlation between WLB and compassion satisfaction (CS) and a negative correlation between ST and PS. The study found that the nature of the job, CS, and PD shall predict WLB.

Conclusion:
Stress, burnout, ProQoL, and WLB of MHPs vary significantly by job, income, and staying with family, indicating that MHPs are equally vulnerable to stress and burnout and imbalanced WLB, which require appropriate interventions.
Clinical and pathological characteristics of early-onset colorectal cancer in South Korea
Authors: Park Su Bee , Yoon Jin Young , Kwak Min Seob , Cha Jae Myung 
Published: July 2023

Abstract

Background:
Early-onset colorectal cancer (EOCRC) may differ by race and ethnicity, and recently South Korea has witnessed a surge in cases. We aimed to evaluate the clinical and pathological features of patients with EOCRC, and to determine the predictors of overall survival.

Methods:
In this retrospective study, EOCRC was defined as CRC diagnosed in patients aged < 50 years, and late-onset CRC was defined as CRC diagnosed in those over 75 years of age. The clinical and pathological characteristics of patients with EOCRC were compared with late-onset CRC. We also used multivariable Cox proportional hazard models to find predictors of overall survival in patients with EOCRC.

Results:
The proportion of early-onset CRC was 9.1% of 518 patients with CRC, and the clinical and pathological characteristics were similar between early-onset (n = 47) and late-onset CRC (n = 134). However, EOCRC had a preponderance for distal tumor location (70.2% vs. 50.7%, P = 0.02) and T1-2 stage disease (23.4% vs. 11.2%, P = 0.04), compared with those of late-onset CRC. Using multivariable Cox proportional hazard models, only vascular invasion (hazard ratio = 8.75, 95% confidence interval 1.139‒67.197) was found to be a risk factor for overall survival (P = 0.04) for patients with CRC.

Conclusion:
EOCRC had preponderance for distal tumor location and early T-stage disease, compared with late-onset CRC. Considering the increasing incidence of EOCRC, more studies on clinical and pathological characteristics of EOCRC may be warranted.
Radiofrequency-assisted, laparoscopic, clampless partial nephrectomy in patients with low-complexity small renal tumors: A retrospective cohort study
Authors: Zachos Ioannis , Vlachostergios Panagiotis , Mitrakas Lampros , Karatzas Anastasios , Oeconomou Athanasios , Mamoulakis Charalampos , Tzortzis Vasileios 
Published: July 2023

Background:
This single-center, retrospective study was performed to investigate the safety and efficacy of radiofrequency-assisted (RF), laparoscopic partial nephrectomy (PN) with zero ischemia in patients with low-complexity small renal tumors.

Materials and Methods:
Patients with small renal masses (SRMs) who underwent laparoscopic, clampless laparoscopic partial nephrectomy - radiofrequency assisted (LPN-RFA) between January 2016 and June 2020 were studied. Demographics, clinical and pathological characteristics, recurrence-free survival, and overall survival were recorded.

Results:
Fifty-two SRMs were excised from corresponding patients using RFA–LPN. The median tumor size was 2.5 cm and all specimens involved low-complexity masses according to the renal nephrometry score. No conversions to radical nephrectomy were recorded. Postoperatively, there were one patient with fever, one with hematuria, and two with urinary leakage treated endoscopically. The majority of tumors (48/52, 86.2%) were clear-cell carcinomas. According to the glomerular filtration rate postoperatively and 12 months’ posttreatment, adequate renal function was preserved in all patients. There were no positive surgical margins identified postoperatively and no recurrences during a median follow-up 24 months. All patients were alive at the last follow-up.

Conclusions:
This study suggests that RFA laparoscopic clampless PN represents an effective method for managing patients with low-complexity SRMs. It offers adequate intraoperative safety and excellent mid-term oncological control and functional preservation.
Comparative Assessment of Colon Cancer Classification Using Diverse Deep Learning Approaches
Authors: Pavan Kumar V. T. Ram , Arulselvi M. , Sastry K. B. S. 
Published: July 2023

Colon cancer is a general form of avoidable cancer, which is also widely spread across the globe. It is also a leading cancer and considered as big killer among all kinds of cancers. In recent times, significant advances are developed in treatment field of this frequently causing disease. In this research several deep learning techniques namely convolutional neural network (CNN), recurrent neural network (RNN), transfer learning, AlexNet and GoogLeNet are compared for colon cancer classification. Pre-processing is conducted utilizing median filter for removing noises from an input colon cancer image. The filtered image is then segmented using SegNet, which is utilized to segment the affected portions. Finally, classification of colon cancer is conducted employing various deep learning approaches like CNN, RNN, transfer learning, AlexNet and GoogLeNet. The comparative assessment showed GoogLeNet as the best classifier for colon cancer classification with maximal values of accuracy as 94.165, sensitivity as 97.589 and specificity as 87.359 respectively for 60% training data.

Received: 9 June 2023 | Revised: 13 July 2023 | Accepted: 18 July 2023

Conflicts of Interest
The authors declare that they have no conflicts of interest to this work.