bluuebunny/crossref_metadata_2025_split huggingface dataset:
In silico analysis to identify miR-1271-5p/PLCB4 (phospholipase C Beta 4) axis mediated oxaliplatin resistance in metastatic colorectal cancer
Authors: Lee Cheng-Chin , Lee Ai-Wei , Wei Po-Li , Liu Yi-Shin , Chang Yu-Jia , Huang Chien-Yu 
Published: March 2023

AbstractOxaliplatin (OXA) is the first-line chemotherapy drug for metastatic colorectal cancer (mCRC), and the emergence of drug resistance is a major clinical challenge. Although there have been numerous studies on OXA resistance, but its underlying molecular mechanisms are still unclear. This study aims to identify key regulatory genes and pathways associated with OXA resistance. The Gene Expression Omnibus (GEO) GSE42387 dataset containing gene expression profiles of parental and OXA-resistant LoVo cells was applied to explore potential targets. GEO2R, STRING, CytoNCA (a plug-in of Cytoscape), and DAVID were used to analyze differentially expressed genes (DEGs), protein–protein interactions (PPIs), hub genes in PPIs, and gene ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. R2 online platform was used to run a survival analysis of validated hub genes enriched in KEGG pathways. The ENCORI database predicted microRNAs for candidate genes. A survival analysis of those genes was performed, and validated using the OncoLnc database. In addition, the 'clusterProfiler' package in R was used to perform gene set enrichment analysis (GSEA). We identified 395 DEGs, among which 155 were upregulated and 240 were downregulated. In total, 95 DEGs were screened as hub genes after constructing the PPI networks. Twelve GO terms and three KEGG pathways (steroid hormone biosynthesis, malaria, and pathways in cancer) were identified as being significant in the enrichment analysis of hub genes. Twenty-one hub genes enriched in KEGG pathways were defined as key genes. Among them AKT3, phospholipase C Beta 4 (PLCB4), and TGFB1 were identified as OXA-resistance genes through the survival analysis. High expressions of AKT3 and TGFB1 were each associated with a poor prognosis, and lower expression of PLCB4 was correlated with worse survival. Further, high levels of hsa-miR-1271-5p, which potentially targets PLCB4, were associated with poor overall survival in patients with CRC. Finally, we found that PLCB4 low expression was associated with MAPK signaling pathway and VEGF signaling pathway in CRC. Our results demonstrated that hsa-miR-1271-5p/PLCB4 in the pathway in cancer could be a new potential therapeutic target for mCRC with OXA resistance.
Anti-proliferative effect of melatonin in human hepatoma HepG2 cells occurs mainly through cell cycle arrest and inflammation inhibition
Authors: Nabih Heba K. , Hamed Ahmed R. , Yahya Shaymaa M. M. 
Published: March 2023

AbstractHepatocellular carcinoma (HCC) is the major lethal primary liver malignant worldwide. Although, melatonin has various antitumor bioactivities; there is a requirement for more investigations to elucidate the not discussed effects, and the controversial responses of the treatment with melatonin on targets mediated in HCC. To achieve the aim of the present study, HCC-HepG2 cells were treated with different concentrations of melatonin at various time intervals. The selected minimal proliferation inhibition doses of melatonin were then incubated with cells to examine the arresting effect of melatonin on dividing cells using flow cytometry. Furthermore, the molecular patterns of genes that contributed to apoptosis, drug resistance development, antioxidation, and melatonin crossing were quantified by qRT-PCR. Additionally, the Human inflammation antibody array membrane (40 targets) was used to check the anti-inflammatory effect of melatonin. Our results validated that, melatonin shows anti-proliferative action through preserving cells in G0/G1 phase (P < 0.001) that is associated with a highly significant increase in the expression level of the P53 gene (P < 0.01). On contrary, as a novelty, our data recorded decreases in expression levels of genes involved in the pro-apoptotic pathway; with a significant increase (P < 0.05) in the expression level of an anti-apoptotic gene, Bcl2. Interestingly, we detected observed increases in the expression levels of genes responsible for conferring drug resistance including ABCB1, ABCC1, and ABCC5. Our study proved the anti-inflammatory activity of 1 mM melatonin in HCC-HepG2 cells. Accordingly, we can conclude that melatonin facilitates the anti-proliferation of cells at doses of 1 mM, and 2.5 mM after 24 h. This action is initiated through cell cycle arrest at G0/G1 phase via increasing the expression of P53, but independently on apoptosis. Collectively, melatonin is an effective anti-inflammatory and anti-proliferative promising therapy for the treatment of HCC. However, its consumption should be cautious to avoid the development of drug resistance and provide a better treatment strategy.
CDX-2 expression correlates with clinical outcomes in MSI-H metastatic colorectal cancer patients receiving immune checkpoint inhibitors
Authors: Ziranu Pina , Pretta Andrea , Pozzari Marta , Maccioni Antonio , Badiali Manuela , Fanni Daniela , Lai Eleonora , Donisi Clelia , Persano Mara , Gerosa Clara , Puzzoni Marco , Bardanzellu Fabio , Ambu Rossano , Pusceddu Valeria , Dubois Marco , Cerrone Giulia , Migliari Marco , Murgia Sara , Spanu Dario , Pretta Gianluca , Aimola Valentina , Balconi Francesca , Murru Stefania , Faa Gavino , Scartozzi Mario 
Published: March 2023

AbstractImmune checkpoint inhibitors (ICIs) showed efficacy in metastatic colorectal cancer (mCRC) with mismatch-repair deficiency or high microsatellite instability (dMMR-MSI-H). Unfortunately, a patient’s subgroup did not benefit from immunotherapy. Caudal-related homeobox transcription factor 2 (CDX-2) would seem to influence immunotherapy’s sensitivity, promoting the chemokine (C-X-C motif) ligand 14 (CXCL14) expression. Therefore, we investigated CDX-2 role as a prognostic-predictive marker in patients with mCRC MSI-H. We retrospectively collected data from 14 MSI-H mCRC patients treated with ICIs between 2019 and 2021. The primary endpoint was the 12-month progression-free-survival (PFS) rate. The secondary endpoints were overall survival (OS), PFS, objective response rate (ORR), and disease control rate (DCR). The PFS rate at 12 months was 81% in CDX-2 positive patients vs 0% in CDX-2 negative patients (p = 0.0011). The median PFS was not reached (NR) in the CDX-2 positive group versus 2.07 months (95%CI 2.07–10.8) in CDX-2 negative patients (p = 0.0011). Median OS was NR in CDX-2-positive patients versus 2.17 months (95% Confidence Interval [CI] 2.17–18.7) in CDX2-negative patients (p = 0.026). All CDX-2-positive patients achieved a disease response, one of them a complete response. Among CDX-2-negative patients, one achieved stable disease, while the other progressed rapidly (ORR: 100% vs 0%, p = 0.0005; DCR: 100% vs 50%, p = 0.02). Twelve patients received 1st-line pembrolizumab (11 CDX-2 positive and 1 CDX-2 negative) not reaching median PFS, while two patients (1 CDX-2 positive and 1 CDX-2 negative) received 3rd-line pembrolizumab reaching a median PFS of 10.8 months (95% CI, 10.8–12.1; p = 0.036). Although our study reports results on a small population, the prognostic role of CDX-2 in CRC seems confirmed and could drive a promising predictive role in defining the population more sensitive to immunotherapy treatment. Modulating the CDX-2/CXCL14 axis in CDX-2-negative patients could help overcome primary resistance to immunotherapy.
The clinical influence of nasal surgery on PAP compliance and optimal application among OSA subjects uncomfortable with PAP device wear
Authors: Cha Hyunkyung , Oh Heonjeong , Han Sun A , Kim Seo Young , Kim Jeong Kyou , Park Hae Chan , Han Doo Hee , Kim Dong-Young , Kim Hyun Jik 
Published: March 2023

AbstractThis study aimed to evaluate the alteration of PAP compliance after nasal surgery and to determine the optimal indications of nasal surgery in obstructive sleep apnea (OSA) subjects. Among OSA subjects using PAP devices, 29 subjects who underwent septoturbinoplasty due to nasal obstruction were included and their pre- and postoperative medical and PAP records were reviewed retrospectively. Postoperative autoPAP usage data was further assessed by grouping the compliance (the percentage of days with usage ≥ 4 h) data (group 1: the good compliance group; group 2: the poor compliance group). The data showed that 56% of subjects in group 1 complained of nasal obstruction as the only barrier to using a PAP device and about 89% reported experiencing the efficacy of PAP usage. Both the mean and peak average PAP pressures were significantly reduced in group 1 following nasal surgery. Group 2 had multiple subjective problems that interfered with wearing a PAP device and reported a lack of experiencing the efficacy of PAP usage. Preoperative nasal cavity volume values were smaller and absolute blood eosinophil counts were significantly lower in group 1. The current data demonstrate that nasal surgery might increase the compliance of PAP device wear in OSA subjects who complained of only nasal obstruction as a barrier to wearing PAP and who had small nasal cavity volumes combined with allergic inflammation.
Predicting heart failure onset in the general population using a novel data-mining artificial intelligence method
Authors: Miyashita Yohei , Hitsumoto Tatsuro , Fukuda Hiroki , Kim Jiyoong , Washio Takashi , Kitakaze Masafumi 
Published: March 2023

AbstractWe aimed to identify combinations of clinical factors that predict heart failure (HF) onset using a novel limitless-arity multiple-testing procedure (LAMP). We also determined if increases in numbers of predictive combinations of factors increases the probability of developing HF. We recruited people without HF who received health check-ups in 2010, who were followed annually for 4 years. Using 32,547 people, LAMP was performed to identify combinations of factors of fewer than four factors that could predict the onset of HF. The ability of the method to predict the probability of HF onset based on the number of matching predictive combinations of factors was determined in 275,658 people. We identified 549 combinations of factors for the onset of HF. Then we classified 275,658 people into six groups who had 0, 1–50, 51–100, 101–150, 151–200 or 201–250 predictive combinations of factors for the onset of HF. We found that the probability of HF progressively increased as the number of predictive combinations of factors increased. We identified combinations of variables that predict HF onset. An increased number of matching predictive combinations for the onset of HF increased the probability of HF onset.
Multi-scale morphology characterization of hierarchically porous silver foam electrodes for electrochemical CO2 reduction
Authors: Hoffmann Hendrik , Paulisch-Rinke Melanie Cornelia , Gernhard Marius , Jännsch Yannick , Timm Jana , Brandmeir Carola , Lechner Steffen , Marschall Roland , Moos Ralf , Manke Ingo , Roth Christina 
Published: March 2023

AbstractAg catalysts show high selectivities in the conversion of carbon dioxide to carbon monoxide during the electrochemical carbon dioxide reduction reaction (CO2RR). Indeed, highly catalytically active porous electrodes with increased surface area achieve faradaic conversion efficiencies close to 100%. To establish reliable structure-property relationships, the results of qualitative structural analysis need to be complemented by a more quantitative approach to assess the overall picture. In this paper, we present a combination of suitable methods to characterize foam electrodes, which were synthesised by the Dynamic Hydrogen Bubble Templation (DHBT) approach to be used for the CO2RR. Physicochemical and microscopic techniques in conjunction with electrochemical analyses provide insight into the structure of the carefully tailored electrodes. By elucidating the morphology, we were able to link the electrochemical deposition at higher current densities to a more homogenous and dense structure and hence, achieve a better performance in the conversion of CO2 to valuable products.
Chance‐constrained co‐expansion planning for power systems under decision‐dependent wind power uncertainty
Authors: Yin Wenqian , Feng Shuanglei , Liu Rong‐Peng , Hou Yunhe 
Published: March 2023

AbstractVariability and uncertainty in wind resources pose significant challenges to the expansion planning of wind farms and associated flexible resources. In addition, the spatial smoothing effect, indicating the impact of wind farm scale on aggregated wind power prediction errors, further aggravates the challenge. This paper proposes a chance‐constrained co‐expansion planning method considering the spatial smoothing effect, where the expansion of wind farm capacity, batter energy storage capacity, and power transmission lines are co‐optimized. Specifically, a decision‐dependent uncertainty (DDU) model is established capturing the dependency of wind power uncertainties on wind farm expansion decisions under the spatial smoothing effect. Unlike traditional optimization diagram where decisions are made under only decision‐independent uncertainty (DIU) with fixe properties, properties of decision‐dependent uncertain parameters would be inversely altered by decisions. To effectively tackle the coupling relation between decisions and DDU, DDU‐based chance constraints are formulated in an analytical manner, where the decisions and decision‐dependent uncertain parameters are expressed in a closed form. Eventually, with piecewise linearization of the DDU model and the polynomial approximation of cumulative distribution function of uncertain parameters, the proposed chance‐constrained optimization model with DDU is converted into a mixed‐integer second‐order cone program (MISOCP). Case studies verify the effectiveness of the proposed method.
CO enhancement by magnetohydrodynamic waves
Authors: Skalidis R. , Gkimisi K. , Tassis K. , Panopoulou G. V. , Pelgrims V. , Tritsis A. , Goldsmith P. F. 
Published: March 2023

Context. The formation of molecular gas in interstellar clouds is a slow process, but can be enhanced by gas compression. Magneto-hydrodynamic (MHD) waves can create compressed quasi-periodic linear structures, referred to as striations. Striations are observed at the column densities at which the transition from atomic to molecular gas takes place.
Aims. We explore the role of MHD waves in the CO chemistry in regions with striations within molecular clouds.
Methods. We targeted a region with striations in the Polaris Flare cloud. We conducted a CO J = 2−1 survey in order to probe the molecular gas properties. We used archival starlight polarization data and dust emission maps in order to probe the magnetic field properties and compare against the CO morphological and kinematic properties. We assessed the interaction of compressible MHD wave modes with CO chemistry by comparing their characteristic timescales.
Results. The estimated magnetic field is 38–76 µG. In the CO integrated intensity map, we observe a dominant quasiperiodic intensity structure that tends to be parallel to the magnetic field orientation and has a wavelength of approximately one parsec. The periodicity axis is ~17° off from the mean magnetic field orientation and is also observed in the dust intensity map. The contrast in the CO integrated intensity map is ~2.4 times higher than the contrast of the column density map, indicating that CO formation is enhanced locally. We suggest that a dominant slow magnetosonic mode with an estimated period of 2.1–3.4 Myr and a propagation speed of 0.30–0.45 km s−1 is likely to have enhanced the formation of CO, hence created the observed periodic pattern. We also suggest that within uncertainties, a fast magnetosonic mode with a period of 0.48 Myr and a velocity of 2.0 km s−1 could have played some role in increasing the CO abundance.
Conclusions. Quasiperiodic CO structures observed in striation regions may be the imprint of MHD wave modes. The Alfvénic speed sets the dynamical timescales of the compressible MHD modes and determines which wave modes are involved in the CO chemistry.
Revisiting the alignment of radio galaxies in the ELAIS-N1 field
Authors: Simonte M. , Andernach H. , Brüggen M. , Best P. N. , Osinga E. 
Published: March 2023

Aims. Previous studies reported an alignment of the major axes of radio galaxies on various angular scales. Here, we study the alignment of radio galaxies in the ELAIS-N1 Low Frequency ARray (LOFAR) deep field, which covers an area of 25 deg2.
Methods. The low noise level of about 20 μJy beam−1 of the LOFAR deep field observations at 150 MHz enabled the identification of 447 extended (> 30″) radio galaxies for which we measured the position angle of the major axis. We find that 95% of these sources have either photometric or spectroscopic redshifts, which we then used for a three-dimensional (3D) analysis.
Results. We show the distribution of the position angles of radio galaxies in the ELAIS-N1 field and the results of multiple statistical tests carried out to decipher whether the radio galaxies are randomly oriented. We find that the distribution of position angles is consistent with being uniform. Two peaks around position angles of 50 and 140 deg are spurious and are not caused by an alignment, as shown by a 3D analysis. In conclusion, our results do not support a 2D or 3D alignment of radio galaxies on scales of smaller than ∼4 deg.
Characterisation of the new target of the NASA Lucy mission: Asteroid 152830 Dinkinesh (1999 VD57)
Authors: de León J. , Licandro J. , Pinilla-Alonso N. , Moskovitz N. , Kareta T. , Popescu M. 
Published: March 2023

Context. The NASA Lucy mission is designed to collect data that will be used to study the very interesting population of Jupiter Trojans, which are considered to be time capsules from the time of birth of our Solar System. During its journey, the mission will pass near a main belt asteroid, Donaldjohanson. Recently, NASA announced that a new asteroid in the belt will also be visited by Lucy: 152830 Dinkinesh (1999 VD57).
Aims. The main goal of this work is to characterise this newly selected target, asteroid Dinkinesh, in order to provide critical information to the mission team. This information includes the most likely surface composition, albedo, and size of the asteroid, which will be used to better plan the data acquisition strategy at the time of the fly-by.
Methods. To this end, we obtained visible spectra, colour photometry, and time-series photometry for Dinkinesh using several telescopes located at different observatories. For the spectra, we used the 10.4 m Gran Telescopio Canarias (GTC) on the island of La Palma (Spain); for the colour photometry, we used the 4.3 m Lowell Discovery Telescope (LDT) near Happy Jack, Arizona (USA); and for the time-series photometry, we used the 82 cm IAC80 telescope located on the island of Tenerife (Spain). We used the visible reflectance spectrum to obtain the taxonomical class of the asteroid in order to constrain its albedo value. Colour and time-series photometry were used to compute the absolute magnitude of Dinkinesh, which was used in conjunction with an albedo estimation to constrain its size.
Results. Both the visible spectrum and reflectance values computed from colour photometry show that Dinkinesh is an S-type asteroid; that is, it is composed mainly of silicates and some metal. According to observations carried out as part of the NEOWISE survey, S-type asteroids have a typical geometric albedo of pV = 0.223 ± 0.073. From our time-series photometry, we obtain an asteroid mean magnitude of r′ = 19.99 ± 0.05, which provides an absolute magnitude Hr′ = 17.53 ± 0.07 assuming G = 0.19 ± 0.25 for S-types. Using our colour-photometry, we transformed Hr′ to HV = 17.48 ± 0.05. This value of absolute magnitude combined with the geometric albedo provides a mean diameter for Dinkinesh of ~900 m, ranging between a minimum size of 542 m and a maximum size of 1309 m.
Anatomie der Halswirbelsäule
Authors: Mihajlovic Zoran , Hinkelthein Edgar 
Published: March 2023

ZusammenfassungIn der osteopathischen wie auch in der schulmedizinischen
Praxis stellen sich oft Patienten mit einer Mischsymptomatik vor. Der Patient benennt
Symptome, die auf den ersten Blick nicht zusammenpassen. In diesem Artikel sollen die
anatomischen Grundlagen der HWS inklusive ihrer Embryologie zum besseren
Verständnis und zur sichereren Diagnostik- und Therapieplanung dargestellt
werden.
Verkürzte Lebensdauer der Erythrozyten als Determinante der renalen Anämie
Authors: Bissinger Rosi 
Published: March 2023

ZUSAMMENFASSUNGIm Rahmen einer chronischen Nierenerkrankung (CKD: Chronic Kidney Disease) tritt häufig eine renale Anämie auf. Neben einer reduzierten Neubildung von Erythrozyten ist deren verkürzte Lebensdauer eine wichtige Determinante der renalen Anämie. Es konnte gezeigt werden, dass die Erythrozyten über den Mechanismus der Eryptose verfrüht aus der Blutbahn eliminiert werden, ohne dass es zu einer Hämolyse kommt. Dabei exprimieren die Erythrozyten den Marker Phosphatidylserin auf ihrer Oberfläche, was ein potentes Signal zur Phagozytose darstellt. Daraufhin werden die Erythrozyten rasch aus der Blutbahn entfernt. Die Ursache der stimulierten Eryptose bei CKD ist am ehesten auf eine komplexe Störung des inneren Milieus und des Erythrozytenstoffwechsels zurückzuführen.
Rough Sex: Aktueller Diskussions- und Forschungsstand
Authors: Döring Nicola 
Published: March 2023

ZusammenfassungUnter hartem Sex (engl. rough sex) wird aggressiver konsensueller Sex verstanden. Dazu gehören beispielsweise Aktivitäten wie Aufs-Bett-Werfen, Kleider-Herunterreißen, Haare-Ziehen, Schlagen oder Würgen. Rough-Sex-Praktiken sind in den letzten Jahren medial sichtbarer und vermutlich auch populärer geworden. Ihnen sind ganze Pornografie-Gattungen gewidmet und sie werden in Sozialen Medien diskutiert. Große Bevölkerungsteile berichten eigene Erfahrungen mit ausgewählten harten Sexpraktiken. Die Normalisierung von Rough Sex wird mit verschiedenen Gefahren, insbesondere für Frauen, in Verbindung gebracht. Der vorliegende Beitrag fasst den aktuellen Diskussionsstand zusammen, weist auf Forschungslücken hin und diskutiert Konsequenzen für die sexualpädagogische Praxis.
NAl4X4+ (X = S, Se, Te): Clusters with a planar tetracoordinate nitrogen and significantly improved stability
Authors: Sun Rui , Yuan Caixia , Zhai Hua-Jin , Wu Yan-Bo 
Published: March 2023

The design of clusters featuring non-classical planar hypercoordinate atoms (phAs) often depends on the delocalized multicenter bonds involving reactive electron-deficient elements, which both destabilize the clusters and lead to difficulty in achieving the phA arrangement for electronegative elements such as nitrogen due to their preference for localized bonds. In this work, we computationally designed a series of aluminum chalcogenide clusters NAl4X4+ (X = S, Se, Te) with a desired planar tetracoordinate nitrogen and meaningfully improved chemical stability, as evidenced by the wide gaps (6.51–7.23 eV) between their highest occupied molecular orbitals and lowest unoccupied molecular orbitals, high molecular rigidity (dynamically stable up to 1500 K), and exclusively low global energy minima nature (their isomers locate at least 51.2 kcal/mol higher). Remarkably, these clusters are stabilized by peripheral chalcogen atoms, which not only sterically protect the NAl4 core moiety but also electronically compensate for the electron-deficient aluminum atoms via X → Al π back bonds, meeting the description of our recently proposed “electron-compensation” strategy.