bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interrelations and functional roles of key oscillatory activities during daytime sleep in older adults
Authors: Wüst Larissa N. , Antonenko Daria , Malinowski Robert , Khakimova Liliia , Grittner Ulrike , Obermayer Klaus , Ladenbauer Julia , Flöel Agnes 
Published: July 2023

SummaryCertain neurophysiological characteristics of sleep, in particular slow oscillations (SOs), sleep spindles, and their temporal coupling, have been well characterised and associated with human memory abilities. Delta waves, which are somewhat higher in frequency and lower in amplitude compared to SOs, and their interaction with spindles have only recently been found to play a critical role in memory processing of rodents, through a competitive interaction between SO–spindle and delta–spindle coupling. However, human studies that comprehensively address delta wave interactions with spindles and SOs, as well as their functional role for memory are still lacking. Electroencephalographic data were acquired across three naps of 33 healthy older human participants (17 female) to investigate delta–spindle coupling and the interplay between delta‐ and SO‐related activity. Additionally, we determined intra‐individual stability of coupling measures and their potential link to the ability to form novel memories in a verbal memory task. Our results revealed weaker delta–spindle compared to SO–spindle coupling. Contrary to our initial hypothesis, we found no evidence for an opposing dependency between SO‐ and delta‐related activities during non‐rapid eye movement sleep. Moreover, the ratio between SO‐ and delta‐nested spindles rather than SO–spindle and delta–spindle coupling measures by themselves predicted the ability to form novel memories best. In conclusion, our results do not confirm previous findings in rodents on competitive interactions between delta activity and SO–spindle coupling in older adults. However, they support the hypothesis that SO, delta wave, and spindle activity should be jointly considered when aiming to link sleep physiology and memory formation.
Age‐related changes in the pharmacokinetics of meloxicam after intravenous administration in sheep
Authors: Coskun Devran , Corum Orhan , Durna Corum Duygu , Cetin Gul , Irmak Mehmet , Ceyhan Hatice Rumeysa , Uney Kamil 
Published: July 2023

AbstractThe pharmacokinetics of meloxicam was studied in 1‐, 6‐, and 12‐month‐old sheep following a single intravenous (i.v.) dose of 1 mg/kg. The experiments were carried out when the Romanov sheep were 1 month old (7.93 ± 0.91 kg), 6 months old (27.47 ± 4.91 kg), and 12 months old (37.10 ± 3.64 kg). Meloxicam concentration in plasma was determined by high‐performance liquid chromatography and the data collected were evaluated by non‐compartmental kinetic analysis. Meloxicam was detected in the plasma up to 72 h following i.v. administration in all age groups. The volume of distribution at steady state (Vdss) and total body clearance (ClT) were significantly higher in 1‐month‐old (304.87 mL/kg and 16.57 mL/h/kg) than in 12‐month‐old (193.43 mL/kg and 10.50 mL/h/kg) sheep. The area under the concentration–time curve from 0 to 72 h value of meloxicam was lower in 1‐month‐old (58.51 h*μg/mL) compared to 12‐month‐old (92.59 h*μg/mL) sheep. There was no difference in t1/2ʎz value in different age groups. The body extraction ratio values for meloxicam ranged from 0.0186 to 0.0719 after i.v. administration in all age groups. Meloxicam showed an increase in plasma concentration and a decrease in Vdss and ClT in 12‐month‐old compared to 1‐month‐old sheep. Compared to 1‐month‐old and 12‐month‐old sheep, there was no difference in these parameters in 6‐month‐old sheep. Because the age of sheep has an influence on the pharmacokinetics of meloxicam, dosage apparently may need to be adjusted for age.
Compartment‐specific Ca2+ imaging in the green alga Chlamydomonas reinhardtii reveals high light‐induced chloroplast Ca2+ signatures
Authors: Pivato Matteo , Grenzi Matteo , Costa Alex , Ballottari Matteo 
Published: July 2023

Summary
To investigate the role of intracellular Ca2+ signaling in the perception and response mechanisms to light in unicellular microalgae, the genetically encoded ratiometric Ca2+ indicator Yellow Cameleon (YC3.6) was expressed in the model organism for green algae Chlamydomonas reinhardtii, targeted to cytosol, chloroplast, and mitochondria.
Through in vivo single‐cell confocal microscopy imaging, light‐induced Ca2+ signaling was investigated in different conditions and different genotypes, including the photoreceptors mutants phot and acry. A genetically encoded H2O2 sensor was also adopted to investigate the possible role of H2O2 formation in light‐dependent Ca2+ signaling.
Light‐dependent Ca2+ response was observed in Chlamydomonas reinhardtii cells only in the chloroplast as an organelle‐autonomous response, influenced by light intensity and photosynthetic electron transport. The absence of blue and red‐light photoreceptor aCRY strongly reduced the light‐dependent chloroplast Ca2+ response, while the absence of the blue photoreceptor PHOT had no significant effects. A correlation between high light‐induced chloroplast H2O2 gradients and Ca2+ transients was drawn, supported by H2O2‐induced chloroplast Ca2+ transients in the dark.
In conclusion, different triggers are involved in the light‐induced chloroplast Ca2+ signaling as saturation of the photosynthetic electron transport, H2O2 formation, and aCRY‐dependent light perception.
Global diffusion ofCOVID‐19 policies: The role of geographic, institutional, and cultural cues
Authors: An Brian Y. , Porcher Simon , Tang Shui‐Yan 
Published: July 2023

AbstractIn an unprecedented global crisis, like the COVID‐19 pandemic, when vertical global governance plays a relatively minor role, government leaders must obtain policy information cues from other governments' behaviors horizontally. This study builds on the policy diffusion literature to examine the distinct role of geographic, cultural, and institutional peers in influencing a country's policy adoptions throughout a diffusion episode. We theorize that in the case of COVID‐19, policy‐culture fit enhances policy learning, leading cultural cues to exert a more pronounced diffusion influence than geographic and institutional cues over time. Our empirical analysis leverages worldwide data tracking the daily adoption of nine universal COVID‐19 non‐pharmaceutical policies from January 1, 2020 to June 1, 2021 in nearly 100 countries. We show that geographic and institutional proximity are initial sources of policy cues affecting adoption. But their effects fade over time while that of cultural cues grows in the later stage of a diffusion episode, illustrating the role of policy learning fueled by cultural fit. This research deepens our understanding of policy diffusion dynamics, shedding light on the role of national culture in policymaking and design in comparative policy studies.
Objective measured physical activity and metabolic syndrome score in children and adolescents: The UP&DOWN longitudinal study
Authors: Sánchez‐Delgado Alejandro , Sánchez‐Parente Sandra , Martínez‐Gómez David , Gómez‐Martínez Sonia , Veiga Oscar L. , Marcos Ascensión , Castro‐Piñero José , Pérez‐Bey Alejandro 
Published: July 2023

AbstractIntroductionWe aimed to analyze the cross‐sectional and longitudinal association of physical activity (PA) levels and PA patterns with metabolic syndrome score (MetS) in children and adolescents.MethodsA total of 175 children (82 females) and 188 adolescents (95 females) were included. Objective PA levels and patterns were determined by accelerometry. MetS was computed from waist circumference, systolic blood pressure, triglycerides, high‐density lipoprotein cholesterol, and glucose levels. Different linear regression models were implemented to examine the associations of PA with MetS.ResultsVigorous PA, moderate‐vigorous PA, number of bouts per day in 10 min (N10), and total time in bouts per day in 10 min (T10) were negatively associated with MetS in male children and adolescents at cross‐sectional level (β ranging from −0.005 to −0.164, all p < 0.05). Total time in bouts per day in 20 min in male children, and vigorous PA and N10 in female children were longitudinally and negatively associated with MetS (β ranging from −0.011 to −0.247, all p < 0.05).ConclusionsAssociations of PA and MetS were observed at cross‐sectional level in males and longitudinally in female children. The associations in PA patterns were found when patterns were grouped into bouts of 10 min. Therefore, for future studies of PA with health markers in the pediatric population, it would be advisable to choose bouts of shorter duration.
Of tanks and tankies: What's ‘left’ for geography after the invasion of Ukraine
Authors: Klinke Ian 
Published: July 2023

AbstractThis commentary explores the degree to which the 2022 Russian invasion of Ukraine has posed a challenge for political geographers. I reason that while the war has exposed conceptual weaknesses and political blind spots, it has also validated many of the foci with which geographers have approached post‐Cold War European security. By drawing attention to the epistemological and moral problems of current NATO scepticism, I argue that tactics of counter‐mapping and whataboutery need to be carried out with the necessary care for the historical and political context.
Correlation between canine biochemical analytes and TRP36 ELISA seropositivity for Ehrlichia canis in Brazil
Authors: Zorzo Carolina , Pereira Nathalia A. , Hongyu Kuang , Aguiar Daniel M. 
Published: July 2023

AbstractBackgroundSynthetic peptides of tandem repeat proteins (TRPs) have been employed in the serologic analysis of canine monocytic ehrlichiosis (CME) and used in epidemiological studies in Brazil. Based on molecular studies of TRPs, different genotypes of Ehrlichia canis have been described, but data on their pathogenicity remain unknown.ObjectivesTo correlate hepatic, renal, and muscular alterations in relation to different genotypes of E. canis in naturally exposed dogs using enzyme‐linked immunosorbent assay (ELISA) with TRP19 and TRP36 synthetic protein antigens.MethodsTwo hundred serum samples were subjected to ELISA with the antigens of TRP19 and three genotypes (US, Br, and CR) of TRP36 of E. canis circulating in Brazil. Positive sera were evaluated through eight biochemical parameters, and the results were evaluated by principal component analysis and canonical correlation.ResultsELISA revealed that 47 (23.5%) serum samples reacted to the BrTRP36 peptide, 36 (18%) reacted to the TRP19 peptide, and 8 (4%) reacted to the USTRP36 and CRTRP36 peptides separately. The most frequent biochemical alterations observed were for CK (59.4%), ALB (31.8%), GLO (28.9%), TP (28.9%), ALP (26%), urea (24.6%), creatinine (14.4%), and ALT (14.4%). The most prominent diagnostic method in canonical correlation analysis was BrTRP36, followed by TRP19, which correlated with hyperglobulinemia and hypoalbuminemia.ConclusionsAntibodies that reacted against the Brazilian genotype of E. canis correlated positively with hyperglobulinemia and increases in serum urea and creatinine. According to our results, the Brazilian genotype of E. canis is related to the chronic phase of CME.
Swirl Brake Design for Improved Rotordynamic Vibration Stability Based on Computational Fluid Dynamics System Level Modeling
Authors: Shujan Ali MD , Mortazavi Farzam , Palazzolo Alan 
Published: July 2023

Abstract
The accurate characterization of compressor rotordynamic coefficients during the design phase reduces the risk of subsynchronous vibration problems occurring in the field. Although rotordynamists extensively investigate discrete compressor components (such as seals and front shrouds) to tackle instability issues, integrated or system-level analysis of compressor rotordynamics is very sparse. In reality, the impeller, eye-labyrinth seal, and the front shroud heavily influence one another; and the collective dynamic behavior of the system differs from the sum of the dynamic behavior of isolated components. A computational fluid dynamics (CFD)-based approach is taken to evaluate the dynamic behavior of the system as a whole. The geometry and operating conditions in this work are based on the recent experimental study of Song et al. (2019, “Non-Axisymmetric Flows and Rotordynamic Forces in an Eccentric Shrouded Centrifugal Compressor—Part 1: Measurement,” ASME J. Eng. Gas Turbines Power, 141(11), p. 111014. 10.1115/1.4044874) on centrifugal compressor. The commercial CFD code cfx 19.0 is used to resolve Reynolds-averaged Navier–Stokes equations to quantify the eye-labyrinth seal and front cavity stiffness, damping, and added mass. The entire compressor stage is modeled to uncover the coupled behavior of the components and assess the stability of the whole system instead of just discrete components. In the current work, three CFD approaches, namely quasi-steady, transient static eccentricity, and transient mesh deformation techniques are studied and benchmarked against analytical and experimental results from the literature. Having established the efficacy of the proposed approach, four types of swirl brakes are proposed and analyzed for stability. The novel swirl brakes create negative swirls at the brake cavities and stabilize both the front shroud and the eye-labyrinth seal simultaneously.
The Effects of a Field-Based Priming Session on Perceptual, Physiological, and Performance Markers in Female Rugby Sevens Players
Authors: Mason Billy R.J. , McKune Andrew J. , Pumpa Kate L. , Mara Jocelyn K. , Engel Alexander C. , Kilduff Liam P. , Ball Nick B. 
Published: July 2023

Purpose: This study aimed to determine the effects of a field-based priming session on perceptual, physiological, and performance responses in female rugby sevens athletes. Methods: Thirteen highly trained female rugby sevens players (age: 20.7 [2.0] y; height: 169.3 [4.8] cm; weight: 68.8 [7.9] kg) completed either a 20-minute field-based priming session or a control condition. Perceptual, physiological, and performance variables were collected at baseline (PRE) and 5 (POST5), 30 (POST30), and 120 minutes (POST120) postintervention. Data were analyzed using Bayesian mixed effects models. Results: The priming protocol had a larger increase in mental readiness (maximum a posteriori [MAP] = 20, 95% high-density intervals [HDI] = −4 to 42, probability of direction [PD]% = 95, % in region of practical equivalence [ROPE] = 9.7), physical readiness (MAP = 20.1, 95% HDI = −4.6 to 42.1, PD% = 93, % in ROPE = 10.6), and testosterone (MAP = 14.9, 95% HDI = 0.5 to 27.7, PD% = 98, % in ROPE = 5.6) than the control POST30. Cognitive performance decreased POST120 in the priming condition for congruent (MAP = 0.02, 95% HDI = −0.06 to 0.00, PD% = 95, % in ROPE = 6.4) and incongruent tasks (MAP = 0.00, 95% HDI = −0.07 to 0.00, PD% = 98, % in ROPE = 3.2) when compared with the control. Conclusions: Perceptual and physiological markers improved POST30 in the priming condition. Findings indicate that perceptual and physiological responses to priming were not coupled with performance improvements. Priming was not accompanied by perceptual, physiological, or performance improvements at POST120.
Establishment of a CPER reverse genetics system for Powassan virus defines attenuating NS1 glycosylation sites and an infectious NS1-GFP11 reporter virus
Authors: Conde Jonas N. , Himmler Grace E. , Mladinich Megan C. , Setoh Yin Xiang , Amarilla Alberto A. , Schutt William R. , Saladino Nicholas , Gorbunova Elena E. , Salamango Daniel J. , Benach Jorge , Kim Hwan Keun , Mackow Erich R. 
Published: July 2023

ABSTRACT

Powassan virus (POWV) is an emerging tick-borne Flavivirus that causes lethal encephalitis and long-term neurologic damage. Currently, there are no POWV therapeutics, licensed vaccines, or reverse genetics systems for producing infectious POWVs from recombinant DNA. Using a circular polymerase extension reaction (CPER), we generated recombinant LI9 (recLI9) POWVs with attenuating NS1 protein mutations and a recLI9-split-eGFP reporter virus. NS1 proteins are highly conserved glycoproteins that regulate replication, spread, and neurovirulence. POWV NS1 contains three putative N-linked glycosylation sites that we modified individually in infectious recLI9 mutants (N85Q, N208Q, and N224Q). NS1 glycosylation site mutations reduced replication kinetics and were attenuated, with 1–2 log decreases in titer. Severely attenuated recLI9-N224Q exhibited a 2- to 3-day delay in focal cell-to-cell spread and reduced NS1 secretion but was lethal when intracranially inoculated into suckling mice. However, footpad inoculation of recLI9-N224Q resulted in the survival of 80% of mice and demonstrated that NS1-N224Q mutations reduce POWV neuroinvasion
in vivo
. To monitor NS1 trafficking, we CPER fused a split GFP11-tag to the NS1 C-terminus and generated an infectious reporter virus, recLI9-NS1-GFP11. Cells infected with recLI9-NS1-GFP11 revealed NS1 trafficking in live cells and the novel formation of large NS1-lined intracellular vesicles. An infectious recLI9-NS1-GFP11 reporter virus permits real-time analysis of NS1 functions in POWV replication, assembly, and secretion and provides a platform for evaluating antiviral compounds. Collectively, our robust POWV reverse genetics system permits analysis of viral spread and neurovirulence determinants
in vitro
and
in vivo
and enables the rational genetic design of live attenuated POWV vaccines.

IMPORTANCE

Our findings newly establish a mechanism for genetically modifying Powassan viruses (POWVs), systematically defining pathogenic determinants and rationally designing live attenuated POWV vaccines. This initial study demonstrates that mutating POWV NS1 glycosylation sites attenuates POWV spread and neurovirulence
in vitro
and
in vivo
. Our findings validate a robust circular polymerase extension reaction approach as a mechanism for developing, and evaluating, attenuated genetically modified POWVs. We further designed an infectious GFP-tagged reporter POWV that permits us to monitor secretory trafficking of POWV in live cells, which can be applied to screen potential POWV replication inhibitors. This robust system for modifying POWVs provides the ability to define attenuating POWV mutations and create genetically attenuated recPOWV vaccines.
Supplemental Material: Magma mingling and ascent in the minutes to hours before an explosive eruption as recorded by banded pumice
Authors: Shamloo Hannah I. , Grunder Anita L. 
Published: July 2023

<p>Additional figures with discussion of analytical and modeling methods (File S1: Figs. S1–S6), data collected (File S2: Tables S1–S4), and Python script (File S3). </p>
Infraspinatus-teres minor (ITM) interfascial block: a novel approach for combined suprascapular and axillary nerve block
Authors: Kim Shin Hyung , Yeo In-Seung , Jang Jaewon , Jung Hyun Eom , Chun Yong-Min , Yang Hun-Mu 
Published: July 2023

BackgroundCombined suprascapular and axillary nerve block could be an analgesic option for shoulder pain control. The current description of this technique requires performing the block procedures at two different sites without consideration for catheter placement. We hypothesized that a single site injection to the interfascial plane between the infraspinatus and teres minor would result in an injectate spread to the suprascapular and axillary nerves.MethodsWe performed 10 injections with this approach using 25 mL dye solution in 10 shoulders of five unembalmed cadavers. Also, we described three case reports, two single-injection cases and one catheter-placement case, using this approach in patients with acute postsurgical pain and chronic pain in their shoulder region.ResultsIn cadaveric evaluations, dye spreading to the suprascapular nerves on the infraspinatus fossa and the spinoglenoid notch cephalad and axillary nerves in the quadrilateral space caudally were observed in all injections. In addition, the most posterolateral part of the joint capsule was stained in 8 out of 10 injections. There was no dye spreading on the nerves to the subscapularis or lateral pectoral nerves. Clinically successful analgesia with no adverse events was achieved in all three cases.ConclusionOur anatomical and clinical observations demonstrated that an injection to the interfascial plane between the infraspinatus and teres minor consistently achieved injectate spreading to both suprascapular and axillary nerves, which innervate the glenohumeral joint.
Greybody factors for massive scalar field emitted from black holes in dRGT massive gravity
Authors: Boonserm Petarpa , Phalungsongsathit Sattha , Sansuk Kunlapat , Wongjun Pitayuth 
Published: July 2023

AbstractGreybody factors are transmission probabilities of the Hawking radiation, which are emitted from black holes and can be obtained from the gravitational potential of black holes. The de Rham, Gabadadze, and Tolly (dRGT) massive gravity is one of the gravity theories that modified general relativity. In this paper, we investigate the greybody factor from the massive scalar field in both the asymptotically dS and the AdS spacetime using the WKB and the rigorous bound methods. We found that the greybody factor depends on the shape of the potential as found in quantum mechanics. The higher the potential barrier, the lower the amount of the greybody factor. Interestingly, for the low multipole case, we found that there exists a critical mass which provides the maximum bound of the greybody factor. This is a crucial feature of the massive scalar field on the greybody factor from the black holes in both the asymptotically dS and the AdS spacetime.
L-Space and Large Language Models
Authors: Kaye Jofish 
Published: July 2023

From the intersection of computational science and technological speculation, with boundaries limited only by our ability to imagine what could be.
Design fiction is an approach to understanding and speculating about alternate futures. One part of this can involve creating representative artifacts or prototypes from the future, as if they fell through a time warp to the present day. This column is a piece of such speculative fiction, set in 2025.