bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural basis of spike RBM-specific human antibodies counteracting broad SARS-CoV-2 variants
Authors: Shitaoka Kiyomi , Higashiura Akifumi , Kawano Yohei , Yamamoto Akima , Mizoguchi Yoko , Hashiguchi Takao , Nishimichi Norihisa , Huang Shiyu , Ito Ayano , Ohki Shun , Kanda Miyuki , Taniguchi Tomohiro , Yoshizato Rin , Azuma Hitoshi , Kitajima Yasuo , Yokosaki Yasuyuki , Okada Satoshi , Sakaguchi Takemasa , Yasuda Tomoharu 
Published: April 2023

AbstractThe decrease of antibody efficacy to mutated SARS-CoV-2 spike RBD explains the breakthrough infections and reinfections by Omicron variants. Here, we analyzed broadly neutralizing antibodies isolated from long-term hospitalized convalescent patients of early SARS-CoV-2 strains. One of the antibodies named NCV2SG48 is highly potent to broad SARS-CoV-2 variants including Omicron BA.1, BA.2, and BA.4/5. To reveal the mode of action, we determined the sequence and crystal structure of the Fab fragment of NCV2SG48 in a complex with spike RBD from the original, Delta, and Omicron BA.1. NCV2SG48 is from a minor VH but the multiple somatic hypermutations contribute to a markedly extended binding interface and hydrogen bonds to interact with conserved residues at the core receptor-binding motif of RBD, which efficiently neutralizes a broad spectrum of variants. Thus, eliciting the RBD-specific B cells to the longitudinal germinal center reaction confers potent immunity to broad SARS-CoV-2 variants emerging one after another.
Harmonizing across datasets to improve the transferability of drug combination prediction
Authors: Zhang Hanrui , Wang Ziyan , Nan Yiyang , Zagidullin Bulat , Yi Daiyao , Tang Jing , Guan Yuanfang 
Published: April 2023

AbstractCombination treatment has multiple advantages over traditional monotherapy in clinics, thus becoming a target of interest for many high-throughput screening (HTS) studies, which enables the development of machine learning models predicting the response of new drug combinations. However, most existing models have been tested only within a single study, and these models cannot generalize across different datasets due to significantly variable experimental settings. Here, we thoroughly assessed the transferability issue of single-study-derived models on new datasets. More importantly, we propose a method to overcome the experimental variability by harmonizing dose–response curves of different studies. Our method improves the prediction performance of machine learning models by 184% and 1367% compared to the baseline models in intra-study and inter-study predictions, respectively, and shows consistent improvement in multiple cross-validation settings. Our study addresses the crucial question of the transferability in drug combination predictions, which is fundamental for such models to be extrapolated to new drug combination discovery and clinical applications that arede factodifferent datasets.
Stimulated Raman scattering metrology of molecular hydrogen
Authors: Lamperti Marco , Rutkowski Lucile , Ronchetti Daniele , Gatti Davide , Gotti Riccardo , Cerullo Giulio , Thibault Franck , Jóźwiak Hubert , Wójtewicz Szymon , Masłowski Piotr , Wcisło Piotr , Polli Dario , Marangoni Marco 
Published: April 2023

AbstractFrequency combs have revolutionized optical frequency metrology, allowing one to determine highly accurate transition frequencies of a wealth of molecular species. These progresses have only marginally benefited infrared-inactive transitions, due to their inherently weak cross-sections. Here we overcome this limitation by introducing stimulated-Raman-scattering metrology, where a frequency comb is exploited to calibrate the frequency detuning between the pump and Stokes excitation lasers. We apply this approach to the investigation of molecular hydrogen, which is a recognized benchmark for tests of quantum electrodynamics and of theories that describe physics beyond the standard model. Specifically, we measure the transition frequency of the Q(1) fundamental line of H2 around 4155 cm−1 with few parts-per-billion uncertainty, which is comparable to the theoretical benchmark of ab initio calculations and more than a decade better than the experimental state of the art. Our comb-calibrated stimulated Raman scattering spectrometer extends the toolkit of optical frequency metrology as it can be applied, with simple technical changes, to many other infrared-inactive transitions, over a 50-5000 cm−1 range that covers also purely rotational bands.
Tectonic settings influence the geochemical and microbial diversity of Peru hot springs
Authors: Upin Heather E. , Newell Dennis L. , Colman Daniel R. , Boyd Eric S. 
Published: April 2023

AbstractTectonic processes control hot spring temperature and geochemistry, yet how this in turn shapes microbial community composition is poorly understood. Here, we present geochemical and 16 S rRNA gene sequencing data from 14 hot springs from contrasting styles of subduction along a convergent margin in the Peruvian Andes. We find that tectonic influence on hot spring temperature and geochemistry shapes microbial community composition. Hot springs in the flat-slab and back-arc regions of the subduction system had similar pH but differed in geochemistry and microbiology, with significant relationships between microbial community composition, geochemistry, and geologic setting. Flat-slab hot springs were chemically heterogeneous, had modest surface temperatures (up to 45 °C), and were dominated by members of the metabolically diverse phylum Proteobacteria. Whereas, back-arc hot springs were geochemically more homogenous, exhibited high concentrations of dissolved metals and gases, had higher surface temperatures (up to 81 °C), and host thermophilic archaeal and bacterial lineages.
Muramyl dipeptide CD10 monoclonal antibody immunoconjugates inhibited acute leukemia in nude mice
Authors: Wang Yilin , Jin Xiaofu , Sun Yan , Zhao Yanxia , Qu Zhenghai , Wang Lingzhen , Sun Lirong 
Published: April 2023

Abstract
Minimal residual disease (MRD) is one of the causes of leukemia recurrence. Previously, we developed anti-CD10 mAb conjugated to muramyl dipeptide immunoconjugate (MDP-Ab) for immune enhancement. The present study aimed to investigate anti-leukemia effect of MDP-Ab administered via different methods in leukemia ectopic graft nude mouse model. BALB/c nude mice were injected with Nalm-6 cells subcutaneously to establish leukemia xenografts in nude mice as a model. MDP-Ab or/and human lymphocytes (LYM) was injected into different sites of the nude mice. Immunohistochemistry staining of CDs in the bone marrow, liver and spleen was performed. IFN-γ was detected by ELISA. We detected the metastasis of leukemia cells to the liver, spleen and bone marrow in nude mouse leukemia model. MDP-Ab and LYM inhibited the growth of tumors, and simultaneous injection of MDP-Ab and LYM into the tumor inhibited the growth of tumors. IFN-γ levels in MDP-Ab (ca) + h-LYM (ca) group, MDP-Ab (ca) + h-LYM (ip) group, MDP-Ab (iv) + h-LYM (ip) group and PBS (ca) + h-LYM (ca) group were significantly higher than those in control group, while IFN-γ level in MDP-Ab (ca) + h-LYM (ca) group was the highest. Moreover, MDP-Ab and h-LYM promoted the expression of hCD4 and hCD8, with the highest expression in MDP-Ab (ca) + h-LYM (ca) group. In conclusion, MDP-Ab effectively promoted the production of IFN-γ, enhanced the antitumor immunity of T lymphocytes and inhibited leukemia.
The Role of Speech-Language Pathologists in Expanding Delivery of Teen Online Problem Solving for Adolescents With Acquired Brain Injury: A Quality Improvement Project
Authors: Lundine Jennifer P. , Chitwood Kerrie Lemons , Wade Shari L. 
Published: April 2023

Purpose:
Teen Online Problem Solving (TOPS) is an evidence-based teletherapy program designed to promote neurocognitive, behavioral, and psychosocial recovery following brain injury through family-centered training. To date, TOPS has been primarily administered by neuropsychologists and clinical psychologists. This clinical focus article discusses a quality improvement project to adapt the TOPS training and manual for use by speech-language pathologists (SLPs) and reports feedback from SLPs following TOPS training and after delivering the program with adolescents who experienced neurological insults.

Method:
SLPs were invited to participate in TOPS training. Trainees were asked to complete posttraining surveys, active therapist questionnaires, and follow-up surveys directed to SLPs who had completed the intervention with at least one patient.

Results:
To date, a total of 38 SLPs completed TOPS training, 13 have implemented TOPS with at least one adolescent. Eight SLPs and 16 psychologists/trainees responded to follow-up surveys to share their perspectives on the program. Perceptions of clinicians delivering the program did not differ significantly in most respects. SLPs rated the ease of understanding nonverbal communication higher than psychologists. Seven SLPs responded to an SLP-specific survey about their experiences administering TOPS, noting a range of advantages and some limitations in their open-ended responses.

Conclusion:
Training SLPs to deliver TOPS has the potential to increase service provision to adolescents with acquired brain injury who have cognitive communication difficulties and their families.

Supplemental Material:

https://doi.org/10.23641/asha.22357327
Few‐shot object detection via class encoding and multi‐target decoding
Authors: Guo Xueqiang , Yang Hanqing , Wei Mohan , Ye Xiaotong , Zhang Yu 
Published: April 2023

AbstractThe task of few‐shot object detection is to classify and locate objects through a few annotated samples. Although many studies have tried to solve this problem, the results are still not satisfactory. Recent studies have found that the class margin significantly impacts the classification and representation of the targets to be detected. Most methods use the loss function to balance the class margin, but the results show that the loss‐based methods only have a tiny improvement on the few‐shot object detection problem. In this study, the authors propose a class encoding method based on the transformer to balance the class margin, which can make the model pay more attention to the essential information of the features, thus increasing the recognition ability of the sample. Besides, the authors propose a multi‐target decoding method to aggregate RoI vectors generated from multi‐target images with multiple support vectors, which can significantly improve the detection ability of the detector for multi‐target images. Experiments on Pascal visual object classes (VOC) and Microsoft Common Objects in Context datasets show that our proposed Few‐Shot Object Detection via Class Encoding and Multi‐Target Decoding significantly improves upon baseline detectors (average accuracy improvement is up to 10.8% on VOC and 2.1% on COCO), achieving competitive performance. In general, we propose a new way to regulate the class margin between support set vectors and a way of feature aggregation for images containing multiple objects and achieve remarkable results. Our method is implemented on mmfewshot, and the code will be available later.
Double‐loop settlement monitoring system based on laser collimation
Authors: Liu Zhiwei , Zhang Xueling , Bian Ting , Yang Minglai 
Published: April 2023

AbstractWith the rapid development of urbanization, high‐rise buildings have risen from the ground, but in the long‐term use of the building, there will be settlement, tilt, displacement, and other deformations, which will seriously bring disasters. At present, there is a lack of an application‐level settlement monitoring system for the monitoring of these buildings, and there are problems of low intelligent monitoring level and low monitoring accuracy, so this paper proposes a double‐loop monitoring system based on laser collimation. The system combines distributed computing, spot image processing, embedded computing, laser collimation technology etc., to design settlement monitoring equipment and corresponding software. Finally, the data processing is carried out by the double‐loop calculation algorithm to obtain the sedimentation amount of the analyte. The test results show that the whole system can be intelligently and accurately monitored, and the monitoring accuracy can reach the millimetre level.
Detection and classification of atrial and ventricular cardiovascular diseases to improve the cardiac health literacy for resource constrained regions
Authors: Arora Neha , Mishra Biswajit 
Published: April 2023

AbstractECG is a non‐invasive way of determining cardiac health by measuring the electrical activity of the heart. A novel detection technique for feature points P, QRS and T is investigated to diagnose various atrial and ventricular cardiovascular anomalies with ECG signals for ambulatory monitoring. Before the system is worthy of field trials, it is validated with several databases and recorded their response. The QRS complex detection is based on the Pan Tompkins algorithm and difference operation method that provides positive predictivity, sensitivity and false detection rate of 99.29%, 99.49% and 1.29%, respectively. Proposed novel T wave detection provides sensitivity of 97.78%. Also, proposed P wave detection provides positive predictivity, sensitivity and false detection rate of 99.43%, 99.4% and 1.15% for the control study (normal subjects) and 82.68%, 94.3% and 25.4% for the case (patients with cardiac anomalies) study, respectively. Disease detection such as arrhythmia is based on standard R‐R intervals while myocardial infarction is based on the ST‐T deviations where the positive predictivity, sensitivity and accuracy are observed to be 94.6%, 84.2% and 85%, respectively. It should be noted that, since the frontal leads are only used, the anterior myocardial infarction cases are detected with the injury pattern in lead avl and ST depression in reciprocal leads. Detection of atrial fibrillation is done for both short and long duration signals using statistical methods using interquartile range and standard deviations, giving very high accuracy, 100% in most cases. The system hardware for obtaining the 2 lead ECG signal is designed using commercially available off the shelf components. Small field validation of the designed system is performed at a Public Health Centre in Gujarat, India with 42 patients (both cases and controls). 78.5% accuracy was achieved during the field validation. It is thus concluded that the proposed method is ideal for improvisation in cardiac health monitoring outreach in resource constrained regions.
Vom Vermittlungsparadigma hin zur nachhaltigen Unterstützung
von Unternehmen bei der Eingliederung von Menschen mit hochfunktionalen Autismus
– Ergebnisse einer Arbeitgeberbefragung
Authors: Reich Katrin , Dalferth Matthias 
Published: April 2023

ZusammenfassungIm Rahmen des Projektes AUT-1A wurden 123 Arbeitgeber*innen mittels
Fragebogen zu ihren Erfahrungen mit der Beschäftigung von autistischen
Mitarbeiter*innen befragt. Ziel war es, die
beschäftigungsfördernden und -hindernden Faktoren
herauszuarbeiten. Die Studie deutet darauf hin, dass sich die berufliche
Qualifizierung in den Berufsbildungswerken positiv auf die nachhaltige
Beschäftigung von Menschen mit Autismus-Spektrum-Diagnose (ASD)
auswirkt, die Unterstützungsleistungen für Betriebe aber noch
nicht hinreichend sind. Auch konnte eine mangelnde Aufklärung in Bezug
auf eine autismusfreundliche Umgebungsgestaltung sowie eine mangelnde
Aufklärung über die Diagnose Autismus der direkten
Kolleg*innen herausgearbeitet werden.
Herausforderungen und Lösungsstrategien der beruflichen
Teilhabe von Personen mit Querschnittläsion – Ergebnisse einer
Fokusgruppen-Diskussion
Authors: Bökel Andrea , Geng Veronika , Ostermann Anne , Gutenbrunner Christoph , Egen Christoph 
Published: April 2023

Zusammenfassung
Relevanz Informationen zur Partizipation am Erwerbsleben von Menschen mit
Querschnittläsionen in Deutschland lieferte u. a. der German
Spinal Cord Injury Survey 2017. Offen blieben im quantitativen Forschungsdesign
allerdings Hintergründe und Lösungsmöglichkeiten
bezüglich der genannten Probleme. Diese sollen mit Hilfe einer
Fokusgruppe näher untersucht werden, wobei die Themen Probleme am
Arbeitsplatz, Anerkennung und Vergütung,
Teilzeiterwerbstätigkeit und Gründe für Arbeitslosigkeit
im Vordergrund stehen.
Methodik Die Studie besteht aus einem sequenziellen und vertiefenden
mixed-method Designs zur Komplementarität der quantitativen Daten von
GerSCI 2017 und der qualitativen Daten einer Fokusgruppe mit fünf
Teilnehmenden. Die Auswertung des Transkripts erfolgt anhand der inhaltlich
strukturierenden qualitativen Inhaltsanalyse nach Kuckartz.
Ergebnisse Die Teilnehmenden identifizierten als Gründe
für Erwerbslosigkeit eine aufwendige Bürokratie, eine fehlende
Überleitung von der Rehabilitation in das aktive Berufsleben, fehlende
Beratung sowie die Angst, die finanzielle Unterstützung zu verlieren.
Auch Arbeitgeber:innen, die nicht ausreichend zum Thema Behinderung informiert
waren und Mobbing durch Kolleg:innen wurden als Faktoren identifiziert, die
Teilhabe am Arbeitsleben verhindern. Genannt wurden auch Faktoren, die sich
förderlich auf die Teilhabe am Erwerbsleben auswirken, dazu
gehören die Kooperation von Behandlungszentren für Menschen mit
Querschnittlähmung und potenziellen Arbeitgeber:innen, eine
verlängerte berufliche Erprobungsphase, eine
Transportunterstützung für den Arbeitsweg und finanzielle
Anreize durch Arbeit, sowie Wertschätzung durch Kolleg:innen.
Diskussion Der Weg zurück ins Erwerbsleben sollte sich nicht nur
menschlich lohnen (Kontakte, Sinn, Struktur), sondern muss auch mit einem
finanziellen Anreiz verbunden sein, der sich über dem Niveau der
Kompensationszahlungen der (Sozial-)Versicherungsträger befindet. Das
Erwerbsleben von Menschen mit Behinderung ist eingebunden in einen
größeren gesellschaftlichen Zusammenhang – der Inklusion
– und kein isoliert zu betrachtendes Thema. Die konkreten
Vorschläge der Fokusgruppenteilnehmenden können dabei als
Grundlage für Gespräche von Interessenvertretungen und
politischen Entscheidungsträgern dienen, um das gegenseitige
Verständnis zu fördern.
Technical Variables in Lower Extremity Free Flap Reconstruction
Authors: Christensen Joani M. , Ahn Leah , Meulendijks Mara Z. , Iskhakov David , Wong Frankie , Winograd Jonathan , Valerio Ian L. , Cetrulo Curtis L. , Helliwell Lydia A. , Eberlin Kyle R. 
Published: April 2023

Abstract
Background Lower extremity free flap failure rates are higher than in other areas of the body. While prior studies assessed the effect of intraoperative technical variables, these generally investigated individual variables and did not examine relationships between the many individual technical decisions made during free tissue reconstruction. Our purpose was to investigate the effect of variation in intraoperative microsurgical techniques on flap outcomes in a diverse cohort of patients requiring lower extremity free flap coverage.
Methods Consecutive patients undergoing free flap reconstruction of the lower extremity at two level 1 trauma centers from January 2002 to January 2020 were identified using Current Procedural Terminology codes, followed by a review of medical records. Information regarding demographics and comorbidities, indications, intraoperative technical details, and complications was collected. Outcomes of interest included an unplanned return to the operating room, arterial thrombosis, venous thrombosis, partial flap failure, and total flap failure. Bivariate analysis was performed.
Results In total, 410 patients underwent 420 free tissue transfers. The median follow-up time was 17 months (interquartile ranges: 8.0–37). Total flap failure occurred in 4.9% (n = 20), partial flap failure in 5.9% (n = 24), and unplanned reoperation in 9.0% (n = 37), with arterial thrombosis in 3.2% (n = 13) and venous thrombosis in 5.4% (n = 22). Overall complications were significantly associated with recipient artery choice, with arteries other than PT and AT/DP having a higher rate (p = 0.033), and with arterial revisions (p = 0.010). Total flap failure was also associated with revision of the arterial anastomosis (p = 0.035), and partial flap failure was associated with recipient artery choice (p = 0.032).
Conclusion Many interoperative options and techniques are available when performing microvascular lower extremity reconstruction that leads to equally high success rates. However, the use of arterial inflow outside of the posterior tibial and anterior tibial arteries leads to a higher overall complication rate and partial flap failure rate. Intraoperative revision of the arterial anastomosis portends poorly for ultimate flap survival.
Lower Four Cranial Nerves in the Management of Glomus Jugulare: Anatomical Study
Authors: Fayed Ashraf , El-Deeb Mohamed E. , Magnan Jacques , Meller Renaud , Deveze Arnaud , Elzayat Saad 
Published: April 2023

Abstract
Introduction The surgical management that achieves minimal morbidity and mortality for patients with glomus and non-glomus tumors involving the jugular foramen (JF) region requires a comprehensive understanding of the complex anatomy, anatomic variability, and pathological anatomy of this region.
Objective The aim of this study is to propose a rational guideline to expose and preserve the lower cranial nerves (CNs) in the lateral approach of the JF.
Methods The technique utilized is the gross and microdissection of 4 fixed cadaveric heads to revise the JF's surgical anatomy and high part of the carotid sheath compared with surgical cases to understand and preserve the integrity of lower CNs. The method involves radical mastoidectomy, microdissection of the JF, facial nerve, and high neck just below the carotid canal and the JF. The CNs IX, X, XI, and XII are microscopically dissected and kept in sight up to the JF.
Results This study realized well the surgical and applied anatomy of the lower CNs with relation to the facial nerve and JF.
Conclusions The JF anatomy is complicated, and the key to safely operate on it and preserving the lower CNs is to find the posterior belly of the digastric muscle, to skeletonize the facial nerve, to remove the mastoid tip preserving the stylomastoid foramen, to skeletonize the sigmoid sinus and posterior fossa dura not only anterior but also posteroinferior to reach and drill the jugular tubercle.