bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cenozoic Uplift and Volcanism of Hangai Dome, Central Mongolia Triggered by Lower Mantle Upwellings
Authors: Wu Yingkai , Bao Xuewei 
Published: April 2023

AbstractThe mechanisms of surface uplift of Hangai Dome (>2 km) and formation of Cenozoic intraplate volcanism in central Mongolia remain vigorously debated. Here we directly investigate the mantle transition zone (MTZ) structure beneath central Mongolia to explore whether the deep mantle geodynamical processes have played a role. Our results reveal significant MTZ thinning of 8–13 km in three subareas mainly caused by the uplifted 660‐km discontinuity (∼10 km). In addition, the 410‐km discontinuity is depressed beneath the Hangai Dome (∼6 km) and the northern portion of the study area (10–15 km). These observations provide direct evidences for intracontinental mantle upwellings from the lower to the upper mantle, causing the Cenozoic uplift and basaltic volcanism of central Mongolia. Furthermore, these mantle upwellings are relatively sluggish compared to canonical mantle plumes based on the small magnitude of MTZ thinning and thus relatively moderate thermal anomaly within the MTZ (62–100 K).
Dependence of Quasi‐Electrostatic Magnetosonic Wave Generation on Plasma Density and Suprathermal Protons
Authors: Liu Si , Wang Wenyao , Gao Zhonglei , Yang Qiwu , Xiao Fuliang , He Qian , Li Tong , Zhou Qinghua , Yang Chang , Zhang Sai 
Published: April 2023

AbstractMagnetosonic (MS) wave is one of the most common electromagnetic emissions in the magnetosphere, while quasi‐electrostatic magnetosonic (QEMS) waves are seldom observed. Here we present an interesting QEMS event detected by Van Allen Probe A on 9 January 2014, in which the presence of multi‐band QEMS emissions shows evident correlations with relatively low plasma densities (∼10 cm−3) and enhancements of suprathermal (∼10 − 100 eV) proton fluxes. Based on the observed proton distributions, simulations demonstrate very similar profiles to the observed QEMS in the wave spectral characteristics. Parametric studies indicate that the QEMS growth rate can increase by ∼20 times with the plasma density decreasing from 50 to 10 cm−3. Effective growth rates of higher‐band QEMS occur when the suprathermal proton population is sufficiently large. This study reveals that low densities and large suprathermal proton populations are favorable for generating distinct multi‐band QEMS in the magnetosphere.
Down-regulation of DNA key protein-FEN1 inhibits OSCC growth by affecting immunosuppressive phenotypes via IFN-γ/JAK/STAT-1
Authors: Wang Shimeng , Wang Xiangjian , Sun Jun , Yang Jin , Wu Deyang , Wu Fanglong , Zhou Hongmei 
Published: April 2023

AbstractOral squamous cell carcinoma (OSCC) escape from the immune system is mediated through several immunosuppressive phenotypes that are critical to the initiation and progression of tumors. As a hallmark of cancer, DNA damage repair is closely related to changes in the immunophenotypes of tumor cells. Although flap endonuclease-1 (FEN1), a pivotal DNA-related enzyme is involved in DNA base excision repair to maintain the stability of the cell genome, the correlation between FEN1 and tumor immunity has been unexplored. In the current study, by analyzing the clinicopathological characteristics of FEN1, we demonstrated that FEN1 overexpressed and that an inhibitory immune microenvironment was established in OSCC. In addition, we found that downregulating FEN1 inhibited the growth of OSCC tumors. In vitro studies provided evidence that FEN1 knockdown inhibited the biological behaviors of OSCC and caused DNA damage. Performing multiplex immunohistochemistry (mIHC), we directly observed that the acquisition of critical immunosuppressive phenotypes was correlated with the expression of FEN1. More importantly, FEN1 directly or indirectly regulated two typical immunosuppressive phenotype-related proteins human leukocyte antigen (HLA-DR) and programmed death receptor ligand 1 (PD-L1), through the interferon-gamma (IFN-γ)/janus kinase (JAK)/signal transducer and activator transcription 1 (STAT1) pathway. Our study highlights a new perspective on FEN1 action for the first time, providing theoretical evidence that it may be a potential immunotherapy target for OSCC.
Near-field enhancement of optical second harmonic generation in hybrid gold–lithium niobate nanostructures
Authors: Ali Rana Faryad , Busche Jacob A. , Kamal Saeid , Masiello David J. , Gates Byron D. 
Published: April 2023

AbstractNanophotonics research has focused recently on the ability of nonlinear optical processes to mediate and transform optical signals in a myriad of novel devices, including optical modulators, transducers, color filters, photodetectors, photon sources, and ultrafast optical switches. The inherent weakness of optical nonlinearities at smaller scales has, however, hindered the realization of efficient miniaturized devices, and strategies for enhancing both device efficiencies and synthesis throughput via nanoengineering remain limited. Here, we demonstrate a novel mechanism by which second harmonic generation, a prototypical nonlinear optical phenomenon, from individual lithium niobate particles can be significantly enhanced through nonradiative coupling to the localized surface plasmon resonances of embedded gold nanoparticles. A joint experimental and theoretical investigation of single mesoporous lithium niobate particles coated with a dispersed layer of ~10 nm diameter gold nanoparticles shows that a ~32-fold enhancement of second harmonic generation can be achieved without introducing finely tailored radiative nanoantennas to mediate photon transfer to or from the nonlinear material. This work highlights the limitations of current strategies for enhancing nonlinear optical phenomena and proposes a route through which a new class of subwavelength nonlinear optical platforms can be designed to maximize nonlinear efficiencies through near-field energy exchange.
Sniffing oxytocin: Nose to brain or nose to blood?
Authors: Yao Shuxia , Chen Yuanshu , Zhuang Qian , Zhang Yingying , Lan Chunmei , Zhu Siyu , Becker Benjamin , Kendrick Keith M. 
Published: April 2023

AbstractIn recent years ample studies have reported that intranasal administration of the neuropeptide oxytocin can facilitate social motivation and cognition in healthy and clinical populations. However, it is still unclear how effects are mediated since intranasally administered oxytocin can both directly enter the brain (nose to brain) and increase peripheral vascular concentrations (nose to blood). The relative functional contributions of these routes are not established and have received insufficient attention in the field. The current study used vasoconstrictor pretreatment to prevent intranasal oxytocin (24 IU) from increasing peripheral concentrations and measured effects on both resting-state neural (electroencephalography) and physiological responses (electrocardiogram, electrogastrogram and skin conductance). Results demonstrated that intranasal oxytocin alone produced robust and widespread increases of delta-beta cross-frequency coupling (CFC) from 30 min post-treatment but did not influence peripheral physiological measures. As predicted, vasoconstrictor pretreatment greatly reduced the normal increase in peripheral oxytocin concentrations and, importantly, abolished the majority of intranasal oxytocin effects on delta-beta CFC. Furthermore, time-dependent positive correlations were found between increases in plasma oxytocin concentrations and corresponding increases in delta-beta CFC following oxytocin treatment alone. Our findings suggest a critical role of peripheral vasculature-mediated routes on neural effects of exogenous oxytocin administration with important translational implications for its use as an intervention in psychiatric disorders.
Risk and mortality of testicular cancer in patients with neurodevelopmental or other psychiatric disorders
Authors: Jansson Anna K. , Söderling Jonas , Reutfors Johan , Thor Anna , Sköld Camilla , Cohn-Cedermark Gabriella , Ståhl Olof , Smedby Karin E. , Pettersson Andreas , Glimelius Ingrid 
Published: April 2023

Abstract
Background
Both testicular germ cell tumours (TGCT) and neurodevelopmental disorders are associated with urogenital malformations. Few studies have investigated the association between psychiatric disorders and TGCT. We investigated whether history of any psychiatric or neurodevelopmental disorder is associated with increased risk or mortality of TGCT.

Method
This is a nested case–control study including 6166 TGCT patients diagnosed during 1992–2014, individually matched for age and calendar period to 61,660 controls. We calculated odds ratios (ORs) for the association between type of psychiatric diagnoses and TGCT risk. Among the cases, we used a cohort design and calculated hazard ratios (HRs) of the association between psychiatric diagnose and all-cause and TGCT-specific death.

Results
History of a neurodevelopmental disorder (attention deficit hyperactivity disorder, autism spectrum disorder and intellectual disabilities) was associated with an increased risk of seminoma (OR: 1.54; 1.09–2.19). Seminoma patients with neurodevelopmental disorders were younger (34 versus 38 years, p = 0.004) and had more stage IV disease (5.4% versus 1.2%) than those without. Psychiatric history overall was not associated with TGCT. Patient history of any psychiatric disorder was associated with an increased all-cause and TGCT-specific death.

Conclusions
We report an association between neurodevelopmental disorders and testicular seminoma, and an increased TGCT-specific mortality for TGCT patients with psychiatric disorders.
Butyrate dictates ferroptosis sensitivity through FFAR2-mTOR signaling
Authors: Wang GuoYan , Qin SenLin , Chen Lei , Geng HuiJun , Zheng YiNing , Xia Chao , Yao JunHu , Deng Lu 
Published: April 2023

AbstractEvidence shows that short-chain fatty acids (SCFAs) play an important role in health maintenance and disease development. In particular, butyrate is known to induce apoptosis and autophagy. However, it remains largely unclear whether butyrate can regulate cell ferroptosis, and the mechanism by which has not been studied. In this study, we found that RAS-selective lethal compound 3 (RSL3)- and erastin-induced cell ferroptosis were enhanced by sodium butyrate (NaB). With regard to the underlying mechanism, our results showed that NaB promoted ferroptosis by inducing lipid ROS production via downregulating the expression of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4). Moreover, the FFAR2-AKT-NRF2 axis and FFAR2-mTORC1 axis accounts for the NaB-mediated downregulation of SLC7A11 and GPX4, respectively, in a cAMP-PKA-dependent manner. Functionally, we found that NaB can inhibit tumor growth and the inhibitory effect could be eliminated by administrating MHY1485 (mTORC1 activator) and Ferr-1 (ferroptosis inhibitor). Altogether, in vivo results suggest that NaB treatment is correlated to the mTOR-dependent ferroptosis and consequent tumor growth through xenografts and colitis-associated colorectal tumorigenesis, implicating the potential clinical applications of NaB for future colorectal cancer treatments. Based on all these findings, we have proposed a regulatory mechanism via which butyrate inhibits the mTOR pathway to control ferroptosis and consequent tumorigenesis.
The critical role of apoptosis in mesenchymal stromal cell therapeutics and implications in homeostasis and normal tissue repair
Authors: Giacomini Chiara , Granéli Cecilia , Hicks Ryan , Dazzi Francesco 
Published: April 2023

AbstractMesenchymal stromal cells (MSCs) have been extensively tested for the treatment of numerous clinical conditions and have demonstrated good safety but mixed efficacy. Although this outcome can be attributed in part to the heterogeneity of cell preparations, the lack of mechanistic understanding and tools to establish cell pharmacokinetics and pharmacodynamics, as well as the poorly defined criteria for patient stratification, have hampered the design of informative clinical trials. We and others have demonstrated that MSCs can rapidly undergo apoptosis after their infusion. Apoptotic MSCs are phagocytosed by monocytes/macrophages that are then reprogrammed to become anti-inflammatory cells. MSC apoptosis occurs when the cells are injected into patients who harbor activated cytotoxic T or NK cells. Therefore, the activation state of cytotoxic T or NK cells can be used as a biomarker to predict clinical responses to MSC treatment. Building on a large body of preexisting data, an alternative view on the mechanism of MSCs is that an inflammation-dependent MSC secretome is largely responsible for their immunomodulatory activity. We will discuss how these different mechanisms can coexist and are instructed by two different types of MSC “licensing”: one that is cell-contact dependent and the second that is mediated by inflammatory cytokines. The varied and complex mechanisms by which MSCs can orchestrate inflammatory responses and how this function is specifically driven by inflammation support a physiological role for tissue stroma in tissue homeostasis, and it acts as a sensor of damage and initiator of tissue repair by reprogramming the inflammatory environment.
Incorporating human dimensions is associated with better wildlife translocation outcomes
Authors: Serota Mitchell W. , Barker Kristin J. , Gigliotti Laura C. , Maher Samantha M. L. , Shawler Avery L. , Zuckerman Gabriel R. , Xu Wenjing , Verta Guadalupe , Templin Elizabeth , Andreozzi Chelsea L. , Middleton Arthur D. 
Published: April 2023

AbstractWildlife translocations are increasingly used to combat declining biodiversity worldwide. Successful translocation often hinges on coexistence between humans and wildlife, yet not all translocation efforts explicitly include human dimensions (e.g., economic incentives, education programs, and conflict reduction assistance). To evaluate the prevalence and associated outcomes of including human dimensions as objectives when planning translocations, we analyze 305 case studies from the IUCN’s Global Re-Introduction Perspectives Series. We find that fewer than half of all projects included human dimension objectives (42%), but that projects including human dimension objectives were associated with improved wildlife population outcomes (i.e., higher probability of survival, reproduction, or population growth). Translocation efforts were more likely to include human dimension objectives if they involved mammals, species with a history of local human conflict, and local stakeholders. Our findings underscore the importance of incorporating objectives related to human dimensions in translocation planning efforts to improve conservation success.
Individually addressable and spectrally programmable artificial atoms in silicon photonics
Authors: Prabhu Mihika , Errando-Herranz Carlos , De Santis Lorenzo , Christen Ian , Chen Changchen , Gerlach Connor , Englund Dirk 
Published: April 2023

AbstractA central goal for quantum technologies is to develop platforms for precise and scalable control of individually addressable artificial atoms with efficient optical interfaces. Color centers in silicon, such as the recently-isolated carbon-related G-center, exhibit emission directly into the telecommunications O-band and can leverage the maturity of silicon-on-insulator photonics. We demonstrate the generation, individual addressing, and spectral trimming of G-center artificial atoms in a silicon-on-insulator photonic integrated circuit platform. Focusing on the neutral charge state emission at 1278 nm, we observe waveguide-coupled single photon emission with narrow inhomogeneous distribution with standard deviation of 1.1 nm, excited state lifetime of 8.3 ± 0.7 ns, and no degradation after over a month of operation. In addition, we introduce a technique for optical trimming of spectral transitions up to 300 pm (55 GHz) and local deactivation of single artificial atoms. This non-volatile spectral programming enables alignment of quantum emitters into 25 GHz telecommunication grid channels. Our demonstration opens the path to quantum information processing based on implantable artificial atoms in very large scale integrated photonics.
Phosphate availability and implications for life on ocean worlds
Authors: Randolph-Flagg Noah G. , Ely Tucker , Som Sanjoy M. , Shock Everett L. , German Christopher R. , Hoehler Tori M. 
Published: April 2023

AbstractSeveral moons in the outer solar system host liquid water oceans. A key next step in assessing the habitability of these ocean worlds is to determine whether life’s elemental and energy requirements are also met. Phosphorus is required by all known life and is often limited to biological productivity in Earth’s oceans. This raises the possibility that its availability may limit the abundance or productivity of Earth-like life on ocean worlds. To address this potential problem, here we calculate the equilibrium dissolved phosphate concentrations associated with the reaction of water and rocks—a key driver of ocean chemical evolution—across a broad range of compositional inputs and reaction conditions. Equilibrium dissolved phosphate concentrations range from 10−11 to 10−1 mol/kg across the full range of carbonaceous chondrite compositions and reaction conditions considered, but are generally > 10−5 mol/kg for most plausible scenarios. Relative to the phosphate requirements and uptake kinetics of microorganisms in Earth’s oceans, such concentrations would be sufficient to support initially rapid cell growth and construction of global ocean cell populations larger than those observed in Earth’s deep oceans.
Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography
Authors: Mehrabi Pedram , Sung Sihyun , von Stetten David , Prester Andreas , Hatton Caitlin E. , Kleine-Döpke Stephan , Berkes Alexander , Gore Gargi , Leimkohl Jan-Philipp , Schikora Hendrik , Kollewe Martin , Rohde Holger , Wilmanns Matthias , Tellkamp Friedjof , Schulz Eike C. 
Published: April 2023

AbstractWe introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals are kept in a humidity and temperature-controlled environment, then reactions are initiated via the liquid application method (LAMA) and plunging into liquid nitrogen is initiated after an electronically set delay time to cryo-trap intermediate states. High-magnification images are automatically recorded before and after droplet deposition, prior to plunging. The SPINE-standard sample holder is directly plunged into a storage puck, enabling compatibility with high-throughput infrastructure. Here we demonstrate binding of glucose and 2,3-butanediol in microcrystals of xylose isomerase, and of avibactam and ampicillin in microcrystals of the extended spectrum beta-lactamase CTX-M-14. We also trap reaction intermediates and conformational changes in macroscopic crystals of tryptophan synthase to demonstrate that the spitrobot enables insight into catalytic events.
Low complexity domains of the nucleocapsid protein of SARS-CoV-2 form amyloid fibrils
Authors: Tayeb-Fligelman Einav , Bowler Jeannette T. , Tai Christen E. , Sawaya Michael R. , Jiang Yi Xiao , Garcia Gustavo , Griner Sarah L. , Cheng Xinyi , Salwinski Lukasz , Lutter Liisa , Seidler Paul M. , Lu Jiahui , Rosenberg Gregory M. , Hou Ke , Abskharon Romany , Pan Hope , Zee Chih-Te , Boyer David R. , Li Yan , Anderson Daniel H. , Murray Kevin A. , Falcon Genesis , Cascio Duilio , Saelices Lorena , Damoiseaux Robert , Arumugaswami Vaithilingaraja , Guo Feng , Eisenberg David S. 
Published: April 2023

AbstractThe self-assembly of the Nucleocapsid protein (NCAP) of SARS-CoV-2 is crucial for its function. Computational analysis of the amino acid sequence of NCAP reveals low-complexity domains (LCDs) akin to LCDs in other proteins known to self-assemble as phase separation droplets and amyloid fibrils. Previous reports have described NCAP’s propensity to phase-separate. Here we show that the central LCD of NCAP is capable of both, phase separation and amyloid formation. Within this central LCD we identified three adhesive segments and determined the atomic structure of the fibrils formed by each. Those structures guided the design of G12, a peptide that interferes with the self-assembly of NCAP and demonstrates antiviral activity in SARS-CoV-2 infected cells. Our work, therefore, demonstrates the amyloid form of the central LCD of NCAP and suggests that amyloidogenic segments of NCAP could be targeted for drug development.
Immune cellular patterns of distribution affect outcomes of patients with non-small cell lung cancer
Authors: Parra Edwin Roger , Zhang Jiexin , Jiang Mei , Tamegnon Auriole , Pandurengan Renganayaki Krishna , Behrens Carmen , Solis Luisa , Haymaker Cara , Heymach John Victor , Moran Cesar , Lee Jack J. , Gibbons Don , Wistuba Ignacio Ivan 
Published: April 2023

AbstractStudying the cellular geographic distribution in non-small cell lung cancer is essential to understand the roles of cell populations in this type of tumor. In this study, we characterize the spatial cellular distribution of immune cell populations using 23 makers placed in five multiplex immunofluorescence panels and their associations with clinicopathologic variables and outcomes. Our results demonstrate two cellular distribution patterns—an unmixed pattern mostly related to immunoprotective cells and a mixed pattern mostly related to immunosuppressive cells. Distance analysis shows that T-cells expressing immune checkpoints are closer to malignant cells than other cells. Combining the cellular distribution patterns with cellular distances, we can identify four groups related to inflamed and not-inflamed tumors. Cellular distribution patterns and distance are associated with survival in univariate and multivariable analyses. Spatial distribution is a tool to better understand the tumor microenvironment, predict outcomes, and may can help select therapeutic interventions.
Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development
Authors: Gao Ge , Li Xiaoping , Jiang Zhixin , Osorio Liliana , Tang Ying Lam , Yu Xueqing , Jin Guoxiang , Zhou Zhongjun 
Published: April 2023

AbstractThe outgrowth of epithelial bud followed by reiterated bifurcations during renal development is driven by the ligand-receptor interactions between the epithelium and the surrounding mesenchyme. Here, by exploring ligand-receptor interactions in E10.5 and E11.5 kidneys by single cell RNA-seq, we find that Isthmin1 (Ism1), a secreted protein, resembles Gdnf expression and modulates kidney branching morphogenesis. Mice deficient for Ism1 exhibit defective ureteric bud bifurcation and impaired metanephric mesenchyme condensation in E11.5 embryos, attributable to the compromised Gdnf/Ret signaling, ultimately leading to renal agenesis and hypoplasia/dysplasia. By HRP-induced proximity labelling, we further identify integrin α8β1 as a receptor of Ism1 in E11.5 kidney and demonstrate that Ism1 promoted cell-cell adhesion through interacting with Integrin α8β1, the receptor whose activation is responsible for Gdnf expression and mesenchyme condensation. Taken together, our work reveals Ism1 as a critical regulator of cell-cell interaction that modulates Gdnf/Ret signaling during early kidney development.