bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leveraging a necroptosis pattern to predict the prognosis and drug sensitivity of ovarian cancer
Authors: Su Na , Dou Zhi-Qiang , Yin Xiao-Xue , Xu Hong-Min , Li Chang-Zhong 
Published: March 2023

Abstract
Background: Ovarian cancer (OC) remains a fatal gynecological malignancy. Necroptosis may be a backup pathway that induces cell death when apoptosis is inhibited. This research aims to develop and validate an OC prognosis model based on necroptosis.
Methods: The Cancer Genome Atlas (TCGA) and Genome Tissue Expression Consortium Project Genome (GTEx) databases were used in obtaining data on OC patients and normal ovarian tissues. Necroptosis-related genes were downloaded from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Differentially expressed genes (DEGs) between tumors and normal tissues were screened. COX regression analysis was used in constructing gene signature, which was further tested and validated in the TCGA and GEO cohorts. Based on risk scores and different clinical prognostic features, Nomogram was constructed to predict OS in OC patients. Then, the patients were divided into high- and low-risk groups, and differential genes were identified between the two groups. The potential biological and pathological functions of the differential genes were explored through Gene Ontology (GO) and KEGG analyses. Immunoassays were used to analyze immune status. Immunohistochemistry (IHC) further confirmed the expression levels of core prognostic genes and their correlations with overall survival (OS) rates. Drug sensitivity analysis in different risk groups was performed to screen out potential drugs for the treatment of OC. Finally, a consensus clustering analysis was used in subtyping ovarian tumors.
Results: A three-gene signature was identified, including JAK1, PYGB, and STAT1. The high-risk group had lower OS than the low-risk group and the risk score was acceptable for predicting prognosis independent of any other clinical prognostic features. The Nomogram can accurately predict the 1-, 3-, and 5-year survival rates of patients with OC. Functional analysis revealed immune-related pathways and differences in immune status between the two risk groups. Furthermore, three core prognostic genes involved in model construction were overexpressed in OC versus those in normal ovarian tissues. Patients in the low-risk group were more sensitive to cisplatin and docetaxel. In consensus clustering analysis, OC patients were separated into two subtypes and the survival rate in cluster 1 was better than in cluster 2.
Conclusion: A necroptosis-related model based on three core prognostic DEGs can be used to predict OC prognosis.
Analysis of the Mechanical Properties of Resinated Pinus Pinaster Timber
Authors: Ruy Mônica , Barral María Portela , Conde Inés Seoane , Lorenzana José Antonio , Lombao Maria Belen Feijoo , Sanches Anxel Vidal , Fernandez Manuel Guaita 
Published: March 2023

Abstract
In a global scenario of increasing the use of wood as a structure, the idea of making quality timber production compatible with other forestry activities, including resin extraction, has arisen. Implementing resin extraction practices can bring various socioeconomic benefits to forest communities during tree growing. However, the effects of resin extraction on wood quality are still unknown. Within this context, this research aimed to evaluate the quality of Pinus pinaster timber from resing trees. A total of 238 boards from resin-treated and control trees from two plots located in Galicia were evaluated. Non-destructive and bending tests were carried out according to European standards. The influence of the resin activity on the quality of wood was statistically analyzed. Statistically significant differences between samples of resin and control samples were analyzed for non-destructive, strength, modulus of elasticity, and density parameters. In general, it was observed that the resin activity did not affect the parameters analyzed.
On the Extraction of Amicable Pairs Between Ibn Sīnā- al-Baghdādī and al-Kāshī
Authors: Annaby Mahmoud 
Published: March 2023

Abstract
In this note, we briefly analyse the works Ibn Sīnā (c.980-1037), al-Baghdādī (c.980-1037) and al-Kāshī (d. c. 1429) on the extraction of amicable pairs. We compare these works in the view of the well-known Thābit ibn Qurrā’s rule. Contrary to the belief that these authors are merely stating variations of the famous Thābit ibn Qurrā’s (d. 901) rule in different manners, we show that their statements are different. We prove that the statements of both ibn Sīnā and al-Baghdādī led to an unsolved conjecture, while al-Kāshī’s statement led to the wrong amicable pair . The conjecture implied by ibn Sīnā and al-Baghdādī statements requires testing big primes corresponding to the known 51 Mersenne primes, and we show that no counterexamples yet exist.
Di-n-butyl phthalate regulates insulin sensitivity in human skeletal muscle cell line through the PI3K-AKT-GLUT4 signaling pathway
Authors: Wen Ao , Shan Dan , Chen Yan , Zhang Jing , Zhou Kunyan 
Published: March 2023

Abstract
Background
Dibutyl phthalate (DBP) exposure is a serious environmental problem causing human health threats in China and is closely related to the development of insulin resistance, but its mechanism is unclear. Skeletal muscle tissue is one of the important tissues involved in insulin resistance, and its molecular mechanisms may involve various links of the insulin signaling cascade in muscle cells.
Methods
In this study, the mRNA and protein expression of the above signaling gene were detected and quantified by Real-time quantitative PCR (RT-qPCR) and Western blotting in human skeletal muscle cell line HSkMC cells, and the InsR-IRS1-PI3K-AKT-GLUT4 signaling pathway as the entry point.
Results
The mRNA levels of InsR, IRS-1, PI3K, AKT2, and GLUT4 were reduced after DBP treatment compared with control cells.DBP and PI3K inhibitor BKMl20 or DBP transfected with PTEN overexpression lentivirus further reduced the mRNA expression levels of PI3K, AKT2, and GLUT4. In addition, DBP treatment also reduced the expression of InsR, IRS-1, PI3K, AKT2, and GLUT4 and the phosphorylation of AKT2, but both DBP with BKMl20 or DBP was transfected with PTEN overexpression.
Conclusions
Based on the above results, this study is the first to find a possible molecular mechanism of DBP in skeletal muscle insulin resistance through regulating the PI3K-dependent insulin signaling pathway of skeletal muscle cells, providing a rationale for the pathophysiological changes in the initiation and progression of insulin resistance caused by DBP.
Modulation of memory consolidation by heroin and heroin conditioned stimuli: roles of expectation and dopamine D1 receptor?
Authors: Francis Travis , Leri Francesco 
Published: March 2023

Abstract

It has been theorized that drugs of abuse have enhancing effects on memory consolidation, but recent evidence suggests that this cognitive effect may be mediated by mode of drug administration (i.e., passive vs active). Hence, two studies were designed to test the hypothesis that modulation of memory consolidation by heroin, and by a heroin conditioned stimulus (CS), may be mediated by a dopamine D1 receptor dependent mechanism of prediction error. Using male Sprague-Dawley rats and the object location memory task, Study 1 employed the D1 antagonist SCH23390 (0, 0.05, 0.10 mg/kg, subcutaneous, SC) to modulate enhancement of memory consolidation induced by post-training passive injections of heroin (1 mg/kg, SC) as well as by exposure to the environment paired with heroin injections (6 pairings, 1 h each, 1 mg/kg). Study 2 investigated the same hypothesis but in animals that could learn to predict heroin because the drug was self-administered (0.05 mg/kg/infusion, intravenous), and further explored whether SCH23390 (0 and 0.1 mg/kg) could prevent memory modulation induced by a change in schedule of self-administration (from fixed to variable ratio). It was found that while repeated passive injections of heroin retained their modulatory effect on memory, when self-administered, heroin enhanced consolidation of object location only at the beginning of self-administration and after a change in schedule. Importantly, SCH23390 blocked memory modulation by heroin when passively administered and when the drug was self-administered on a novel schedule. SCH23390 also blocked conditioned memory modulation induced by post-training exposure to the heroin-paired CS. Taken together, these results suggest that modulation of memory consolidation by unconditioned and conditioned opiate reinforcers may involve a DA D1-dependent mechanism that could be encoding the anticipation of drug effects.
Potential functions for functionally graded transversely isotropic media subjected to thermal source in thermoelastodynamics problems
Authors: Panahi Siavash , Neya Bahram Navayi 
Published: March 2023

Abstract
This paper develops a novel set of displacement temperature potential functions to solve the thermoelastodynamic problems in functionally graded transversely isotropic media subjected to the thermal source. For this purpose, three-dimensional heat and wave equations have been considered to obtain the displacement temperature equations of motion in the case of functionally graded materials. A systematic method is used in the present work to decouple the elasticity and heat equations. For governing equations, one sixth order differential equation and two second order differential equations are obtained. Completeness proof of the solution is also presented using a retarded logarithmic Newtonian potential function for the functionally graded transversely isotropic domain. To verify the obtained solution, in a simpler case, potential functions have been generated for homogeneous transversely isotropic media that coincide with the respective equations. Presented potential functions can be used to solve the problems in various media like infinite and semi-infinite space, beams and columns, plates, shells, and etc. with arbitrary boundary conditions and subjected to arbitrary mechanical and thermal loads.
Profile Changes in Class III Malocclusion using Protraction Facemask in Indonesian Young Patients (Cephalometric Study)
Authors: Halim Himawan , Halim Ivan A. 
Published: March 2023

Background:
The prevalence of Class III malocclusion is highest in Southeast Asia. In countries such as Malaysia and Indonesia, the prevalence is as high as 15.80%. Treatment timing and options are very important in treating Class III malocclusion. A protraction facemask or also known as reverse headgear is one of the most used appliances as an interceptive tool for Class III malocclusion in young patients.

Objectives:
This study aims to evaluate the profile changes in treating Indonesian young patients using a protraction facemask.

Methods:
The sample consisted of 63 subjects, 25 males and 38 females. Lateral cephalograms were traced and analyzed using CephaloMetrics AtoZ™ ver. 12 (Yasunaga Computer Systems, Co. Inc., Hanando-Minami, Fukui, Japan). A total of 16 traditional cephalometric measurements (13 skeletal measurements and 3 dental measurements) were used to describe changes between pretreatment and posttreatment cephalograms. All recorded data were analyzed using IBM SPSS version 21 (IBM Corporation, Armonk, New York, USA).

Results:
SNA (p˂0.030), ANB (p=0.000), and Wits appraisal values (p=0.000) showed significant differences out of all measurements. FMA and occlusal plane also showed changes and indicated that a facemask is helpful with treatment.

Conclusion:
Timing, duration, and patient compliance are very important in the management of Class III skeletal malocclusion using a protraction facemask. The use of facemask therapy among Indonesian pediatric patients resulted in an anterior and downward movement of the maxilla, backward rotation of the mandible, and a better orthopedic response.
QUANTITATIVE ANALYSIS OF CYTISINE IN THERMOPSIS ALTERNIFLORAE USING HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY
Authors: GADHVI KRUPA , MODI KARUNA , SHAH MAMTA 
Published: March 2023

Objective: An optimized high-performance thin-layer chromatography (HPTLC) method has been established for the quantification of cytisine in Thermopsis alterniflorae Regel and Schmalh.
Methods: Alcoholic extract of the aerial parts were prepared using Soxhlet extraction method. Separation was achieved on silica gel 60 F254 HPTLC plates using toluene-ethyl acetate-diethyl amine (7:2:1, v/v) as the mobile phase. The quantitation of cytisine was carried out using densitometric scanning at 545 nm after derivatization using Dragendorff’s reagent.
Results: The linear regression analysis data for the calibration plot showed a good linear relationship (r2 = 0.9849) in the concentration range 10 to 15 µg/spot. The method was validated for precision, repeatability, accuracy, specificity, limit of detection and limit of quantification. The average recovery was 99.0899% indicating good accuracy. The percentage yield of cytisine obtained was 0.5075+0.0135 % w/w.
Conclusion: The proposed HPTLC method was found to be simple, sensitive, accurate, reproducible, and robust.
IN VITRO EVALUATION OF ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF AERIAL PARTS OF PHYLLANTHUS FRATERNUSWEB. IN ADULT EARTHWORMS
Authors: SINGH ASHEM DHANACHANDRA , SOBINA MOIRANGTHEM , KANCHAN CHINGTHAM , DEVI PAONAM SHYAMASAKHI , DEVI NAMEIRAKPAM MEENA 
Published: March 2023

Objective: The present study aimed to evaluate the anthelmintic activity of ethanolic extract of aerial part of Phyllanthus fraternus Web. leaves (EEPF) using adult earthworms (Pheretimaposthuma).
Methods: Adult earthworms (24in number) were divided into 4 groups with 6 worms in each group (n=6). The anthelmintic activity of EEPF at two different doses (25 mg/ml and 50 mg/ml) were evaluated by assessing the time of paralysis (min) and time of death (min) of the earthworms. Albendazole (25 mg/ml) was used as standard and 2% gum acacia as control.
Results: Data were analyzed statistically by ANOVA followed by Bonferroni test. P<0.05 was considered as significant. The result showed that EEPF had significant anthelmintic activity (p<0.001) but less potent than the standard drug albendazole.
Conclusion: EEPF demonstrated significant anthelmintic activity but less potent than the standard drug albendazole. However, further studies with higher doses are required to evaluate the dose-dependent activity and to evaluate the exact mechanism responsible for anthelmintic activity.
DEVELOPMENT AND VALIDATION OF A NEW RP HPLC ANALYTICAL METHOD FOR THE DETERMINATION OF ETODOLAC SUCCINIC ACID CO-CRYSTALS IN SPIKED RABBIT PLASMA
Authors: THOMAS ANITHA , VARKEY JOYAMMA 
Published: March 2023

Objective: The aim of the study was to develop and validate a novel and sensitive HPLC method for the determination of Etodolac content in Etodolac succinic acid co-crystals in spiked rabbit plasma.
Methods: Chromatographic separation was achieved on an Eclipse C18 column (4.6 mm,100 mm, 3.5 μm spherical particles) using acetonitrile: methanol: acetic acid (100%) (50:49:1) as the mobile phase at a flow rate of 0.8 ml/min and monitored at 278 nm. Tinidazole was used as the internal standard. The run time was 6 min. The method was validated to fulfill International Conference on Harmonisation (ICH) guidelines, which included specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision and robustness.
Results: The calibration curve was linear over the concentration range from 2.5 to 15 μg/ml, and the lower limit of detection was 0.3700 μg/ml. and lower limit of quantification was 1.121μg/ml for determination in spiked rabbit plasma. The accuracy and precision of the method were within the acceptable limit of±2% at the lower limit of quantification.
Conclusion: A simple, sensitive, rapid and reproducible RP-HPLC method was developed with short runtime and less flow rate. Statistical analysis of the method proved that this method is suitable for the estimation of Etodolac in Co-crystalin plasma. Hence this method can be employed in the routine assay of the Etodolac Succinic acid co-crystals.
Pengembangan Modul Digital pada Materi Struktur Atom untuk Menumbuhkan Literasi Digital Siswa
Authors: Dewi Citra Citra Ayu , Kusuma Janista Mia , Pahriah Pahriah , Syahri Wilda 
Published: March 2023

Penelitian ini bertujuan untuk menghasilkan modul digital pada materi struktur atom yang dapat menumbuhkan literasi digital siswa. Penelitian ini termasuk ke dalam jenis penelitian dan pengembangan (R&D) dengan model menggunakan pengembangan ADDIE (Analysis, Design, Development, Implementation, and Evaluation). Instrumen yang digunakan berupa angket validasi ahli dan angket respon siswa selama penggunaan modul digital yang dikembangkan.Â Subjek uji yaitu siswa kelas X SMAN 1 Sakra Lombok Timur NTB. Pengumpulan data menggunakan angket validasi ahli dan angket respon siswa. Teknik analisis data menggunakan analisis deskriptif. Hasil analisis menunjukkan bahwa modul digital yang telah dikembangkan menggunakan kvisoft flipbook pada materi struktur atom diperoleh persentase kelayakan sebesar 86 dengan kategori layak dan uji kepraktisan terhadap penggunaan modul digital memberikan respon positif dalam menumbuhkan literasi digital siswa. Sehingga, dapat disimpulkan bahwa modul digital pada materi struktur atom layak digunakan untuk menumbuhkan literasi digital siswa.
Crystallinity of Recycled PET Fibers from Chemical and Mechanical Reprocessing
Authors: Wu Leyao , Lienhart Gavan , Basak Sayan , Jana Sadhan C. , Cavicchi Kevin A. , Eagan James M. 
Published: March 2023

ABSTRACT
This work investigated the effect of isophthalate (iso) content in poly(ethylene terephthalate) (PET) materials on its degree of crystallinity (χ%) and mechanical properties. Melt blends were prepared from virgin (0 iso-wt.%) and bottle-grade (1.7 iso-wt.%) PET and subsequently spun into fibers. The mechanical and crystallinity properties were determined using differential scanning calorimetry (DSC), X-ray diffraction (XRD), and uniaxial tensile testing. The crystallinity results determined from DSC and XRD quantified the relationship between iso-content and χ% in the materials. It was found that melt-mixing of different isophthalate grades had a lesser effect on melting temperature (Tm) and χ% than chemically recycled random copolymers of terephthalate and isophthalate. It was further shown that random copolymers of &lt;0.25 iso-wt.% had comparable crystallinity to the virgin high-modulus low-shrink (HMLS) materials.
Vocational guidance networking: school – college – university
Authors: Дрянных Н.В. , Лаврентьева А.А. , Ракова С.С. 
Published: March 2023

Авторы рассматривают понятия «нетворкинг» и «профориентационный нетворкинг», раскрывают сущность «онлайн» и «real» нетворкинга. В статье подробно раскрывается вариант «real-нетворкинга», успешно реализуемого на базе ФГБОУ ВО «Вологодский государственный университет» в виде комплекса профориентационных мероприятий для абитуриентов. Авторами также уделяется внимание проекту в сфере профориентационного нетворкинга – «Первая профессия» – для обучающихся 8–11 классов общеобразовательных организаций, стартовавшему на территории Вологодской области с 1 февраля 2022 года.
The authors consider the concepts of «networking» and «career-oriented networking», reveal the essence of «online» and «real» networking. The article explains the option of «real-networking», successfully implemented at Vologda State University, in the form of a set of vocational guidance activities for applicants. The authors also pay attention to the project in the field of vocational guidance networking – «The First Profession» for students in grades 8–11 of educational institutions, which started in Vologda Region on February 1, 2022.
К характеристике ценопопуляций Stipa pennata L. в Южно-Уральском заповеднике
Authors: Yusupova O.V. , Abramova L.M. , Gorichev Yury P. 
Published: March 2023

The population characteristic of the steppe species Stipa pennata, rare for the South Ural State Nature Reserve, is given. Two coenopopulations (CP) of European feather grass have been analyzed in the western part of the reserve. The ecotope conditions affected the linear parameters of plants significantly. The weather conditions of the year predetermined the height of the generative shoot with a panicle and the leaf length. The maximum linear parameters have been noted for the plants of CP- 2, located in a relatively shaded area with a higher projective cover of the herbaceous and shrub layers. High values of quantitative parameters have been noted in CP-1, which occupied more arid area. The influence of weather conditions on the phenological rhythms of plants are obvious: the acceleration of growth and development in dry years and the slowdown of these processes in the rainy period. The ontogenetic structure of coenopopulations and their demographic indicators are analyzed and presented. Age structure of coenopopulations of S. pennata has a right-shifted unimodal spectrum with a predominance of middle-aged plants. The total population density varies from 4.1 to 6.4 ind./m2. The age and effectiveness of both populations testify they are young.
Приведена популяционная характеристика ред- кого для Южно-Уральского заповедника степного вида Stipa pennata. Проанализированы две ценопопуляции, произраста- ющие в западной части заповедника. Выявлено более высо- кое в процентном отношении воздействие условий экотопа на линейные параметры растений. Влияние фактора погодных условий года в значительной степени отмечено для высоты генеративного побега с метелкой и длины листа. Максималь- ные значения линейных параметров отмечены для ЦП-2, рас- положенной на относительно затененном участке с более вы- соким проективным покрытием травянистого и кустарникового ярусов. Высокие значения количественных параметров отме- чены в ЦП-1, занимающей более сухой участок. Показано влияние погодных условий на фенологические ритмы расте- ний: ускорение роста и развитие в засушливые годы и замед- ление этих процессов в дождливый период. Охарактеризова- на онтогенетическая структура ценопопуляций и приведены их демографические показатели. Возрастная структура цено- популяций S. pennata имеет правосторонний одновершинный тип спектра с преобладанием средневозрастных растений. Общая плотность варьирует от 4.1 до 6.4 экз./м2. Оценка воз- растности и эффективности обеих популяций показала, что они являются молодыми.