bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic syndrome as a late effect of childhood hematopoietic stem cell transplantation – A thorough statistical evaluation of putative risk factors
Authors: Gerbek Tina , Thomsen Birthe Lykke , Muhic Ena , Christiansen Terkel , Sørensen Kaspar , Ifversen Marianne , Kofoed Klaus , Müller Klaus 
Published: April 2023

AbstractBackgroundMetabolic syndrome (MetS) is frequent among survivors of childhood hematopoietic stem‐cell transplantation (HSCT), but assessment of risk factors is challenged by survivor and participation bias in long‐term follow‐up studies.MethodsA cohort of 395 pediatric patients transplanted between 1980 and 2018 was investigated. MetS was assessed at follow‐up between December 2018 and March 2020. Two composite outcomes ((a) combining MetS and death, (b) combining MetS, death, and nonparticipation) were considered to address the risk of selection bias.ResultsAmong 234 survivors invited to the follow‐up, 96 individuals (median age 27 years) participated. MetS prevalence was 30% among participants. The only significant HSCT risk factor was a variable combining HSCT indication and conditioning with total‐body irradiation (TBI) (p = .0011). Compared to acute leukemias (AL) treated with high‐grade TBI (8–12 Gy), a lower MetS prevalence was seen for nonmalignant diseases treated with no/low‐grade TBI (0–4.5 Gy) (OR = 0.04, 95% confidence interval (CI): 0.00–0.23). Analyses of the composite outcomes indicated overestimation of the effect of high‐grade TBI due to selection bias. Scrutiny showed strong residual confounding between HSCT indication and high‐grade TBI within AL‐patients. The HSCT effect on MetS reflected HSCT effects on high‐density‐lipoprotein (HDL) and triglycerides. Compared to AL treated with high‐grade TBI, nonmalignant diagnoses treated with no/low‐grade TBI had higher HDL (+40%, 95% CI: +21% to +62%) and lower triglyceride (−59%, 95% CI: −71% to −42%).ConclusionThe TBI effect on MetS may be overestimated in follow‐up studies due to selection bias and confounding. The TBI effect was confined to the potentially modifiable MetS criteria HDL and triglyceride.
Low‐order fine roots of Picea asperata have different physiological mechanisms in response to seasonal freeze and freeze–thaw of soil
Authors: Xie L. , Xiao Q. , Yin C. 
Published: April 2023

Abstract

Seasonal soil freezing (F) and freeze–thaw cycles (FTCs) are common natural phenomena in high latitude or altitude areas of the world, and seriously affect plant physiological processes. However, studies on the effect of soil F and FTCs on fine roots are less common, especially in subalpine coniferous forests of western Sichuan, China.

We set up a controlled experiment in growth chambers to explore the effects of F and FTCs on low‐order fine roots of Picea asperata and differential responses of first‐order roots and the first three root orders (1st, 2nd and 3rd order roots combined as a unit).

Soil F and FTCs resulted in serious damage to cell membranes and root vitality of low‐order fine roots, accompanied by increased MDA content and O2·− production. FTCs had a stronger effect than F treatment. In turn, low‐order fine roots are the unit that responds to cold stress. These roots had increased unsaturated fatty acid contents, antioxidant enzyme activities, osmolytes and plant hormones contents when acclimation to cold stress. The first‐order roots were more sensitive to cold stress than the combined first three root orders for several processes (e.g. antioxidant enzymes, osmolytes and hormones) because of their specific structure and physiological activity.

This study explains physiological differences in responses of fine roots of different root orders to seasonal soil freezing, which will improve the understanding of fine root heterogeneity and support agriculture and forest management.
Integrated metabolome and transcriptome analysis provides insights on the floral scent formation in Hydrangea arborescens
Authors: Ke Yanguo , Zhou Yiwei , Lv Yiying , Qi Ying , Wei Huanyu , Lei Yu , Huang Feiyan , Abbas Farhat 
Published: April 2023

AbstractHydrangea (Hydrangea arborescens var. "Annabelle”) flowers are composed of sweet aroma sepals rather than true petals and can change color. Floral volatiles plays important roles in plants, such as attracting pollinators, defending against herbivores, and signaling. However, the biosynthesis and regulatory mechanisms underlying fragrance formation in H. arborescens during flower development remain unknown. In this study, a combination of metabolite profiling and RNA sequencing (RNA‐seq) was employed to identify genes associated with floral scent biosynthesis mechanisms in “Annabelle” flowers at three developmental stages (F1, F2, and F3). The floral volatile data revealed that the “Annabelle” volatile profile includes a total of 33 volatile organic compounds (VOCs), and VOCs were abundant during the F2 stage of flower development, followed by the F1 and F3 stages, respectively. Terpenoids and benzenoids/phenylpropanoids were abundant during the F2 and F1 stages, with the latter being the most abundant, whereas fatty acid derivatives and other compounds were found in large amounts during the F3 stage. According to ultra‐performance liquid chromatography‐tandem mass spectrometer analysis, benzene and substituted derivatives, carboxylic acids and derivatives, and fatty acyls play a significant role in the floral metabolite profile. The transcriptome data revealed a total of 17,461 differentially expressed genes (DEGs), with 7585, 12,795, and 9044 DEGs discovered between the F2 and F1, F3 and F1, and F2 and F3 stages, respectively. Several terpenoids and benzenoids/phenylpropanoids biosynthesis‐related DEGs were identified, and GRAS/bHLH/MYB/AP2/WRKY were more abundant among transcription factors. Finally, DEGs interlinked with VOCs compounds were determined using Cytoscape and k‐means analysis. Our results pave the way for the discovery of new genes, critical data for future genetic studies, and a platform for the metabolic engineering of genes involved in the production of Hydrangea's signature floral fragrance.
Modulation of the heartbeat evoked cortical potential by hypnotizability and hypnosis
Authors: Callara Alejandro Luis , Fontanelli Lorenzo , Belcari Iacopo , Rho Gianluca , Greco Alberto , Zelič Žan , Sebastiani Laura , Santarcangelo Enrica L. 
Published: April 2023

AbstractHypnotizability is a psychophysiological trait measured by scales and associated with several differences, including interoceptive accuracy and the morpho‐functional characteristics of interoception‐related brain regions. The aim of the study was to assess whether the amplitude of the heartbeat evoked cortical potential (HEP), a correlate of interoceptive accuracy, differs in participants with low (lows) and high (highs) hypnotizability scores (assessed by the Stanford Hypnotic Susceptibility Scale, Form A) before and after the induction of hypnosis.ECG and EEG were monitored in 16 highs and 15 lows during an experimental session, including open eyes baseline (B), closed eyes relaxation (R), hypnotic induction (IND), neutral hypnosis (NH), and post session baseline (Post). No significant difference was observed between groups and conditions in autonomic variables. The HEP amplitude was lower in highs than in lows at the right parietal site, likely due to hypnotizability related differences in the functional connection between the right insula and parietal cortex. It increased in highs and decreased in lows across the session, possibly due to the highs' preeminently internally directed attention and to the lows' possible disengagement from the task. Since interoception is involved in several cognitive‐emotional functions, its hypnotizability related differences may contribute to the variability of experience and behavior in daily life.
Continued stabilization of blood collections and transfusions in the United States: Findings from the 2021 National Blood Collection and Utilization Survey
Authors: Free Rebecca J. , Sapiano Mathew R. P. , Chavez Ortiz Joel L. , Stewart Phylicia , Berger James , Basavaraju Sridhar V. 
Published: April 2023

AbstractBackgroundNational Blood Collection and Utilization Surveys (NBCUS) have reported decreases in U.S. blood collections and transfusions since 2008. The declines began to stabilize in 2015–2017, with a subsequent increase in transfusions in 2019. Data from the 2021 NBCUS were analyzed to understand the current dynamics of blood collection and use in the United States.MethodsIn March 2022, all community‐based (53) and hospital‐based (83) blood collection centers, a randomly selected 40% of transfusing hospitals performing 100–999 annual inpatient surgeries, and all transfusing hospitals performing ≥1000 annual inpatient surgeries were sent a 2021 NBCUS survey to ascertain blood collection and transfusion data. Responses were compiled, and national estimates were calculated for the number of units of blood and blood components collected, distributed, transfused, and outdated in 2021. Weighting and imputation were applied to account for non‐responses and missing data, respectively.ResultsSurvey response rates were 92.5% (49/53) for community‐based blood centers, 74.7% (62/83) for hospital‐based blood centers, and 76.3% (2102/2754) for transfusing hospitals. Overall, 11,784,000 (95% confidence interval [CI], 11,392,000–12,177,000) whole blood and apheresis red blood cell (RBC) units were collected in 2021, a 1.7% increase from 2019; 10,764,000 (95% CI, 10,357,000–11,171,000) whole blood‐derived and apheresis RBC units were transfused, a 0.8% decrease. Total platelet units distributed increased by 0.8%; platelet units transfused decreased by 3.0%; plasma units distributed increased by 16.2%; and plasma units transfused increased by 1.4%.DiscussionThe 2021 NBCUS findings demonstrate a stabilization in U.S. blood collections and transfusions, suggesting a plateau has been reached for both.
Faecal immunochemical testing (FIT) in primary care: a follow-up service evaluation
Authors: Ayling Ruth M , Machesney Michael 
Published: April 2023

AimColorectal cancer (CRC) is the fourth most common cancer in the UK. Following National Institute for Health and Care Excellence guidance for faecal immunochemical testing (FIT), we introduced a service for the measurement of faecal haemoglobin (f-Hb) in symptomatic patients. Previously, we evaluated the first 6 months of the service in three local boroughs, here we re-examine the use of FIT, over a similar 6 months in the two successive years.MethodsPatients who had FIT requested in April–September 2020 and 2021 were studied. Results were obtained from the laboratory information systems and matched with the clinical outcomes of those referred via the urgent lower gastrointestinal cancer pathway. Patient demographics, reason for referral, clinical outcome and diagnostic test performance are reported.ResultsIn 2020, 4042 samples were analysed and 57 CRC detected. In 2021, 10 508 samples were analysed and 65 CRC detected. Six (4.9%) patients with CRC had f-Hb <10 µg/g, of whom three were anaemic. In 2020, 27.7% of samples were from patients under 50 years; and in 2021, 32.8%. Sensitivity, specificity, positive predictive value and negative predictive value of f-Hb at ≥10 µg/g for CRC were 92.9%, 46.6%, 6.4% and 99.4% in 2020 and 96.9%, 29.9%, 3.2% and 99.8% in 2021.ConclusionsAs currently used in primary care in North East London, specificity of FIT at a cut-off of 10 µg/g is much lower than in published studies and the impact of this on colorectal services needs to be considered.
“I’m both smoking and vaping”: a longitudinal qualitative study of US young adults who tried to quit smoking cigarettes by using electronic cigarettes
Authors: Nguyen Nhung , Koester Kimberly A , Kim Minji , Watkins Shannon Lea , Ling Pamela M 
Published: April 2023

Objective
To describe how young adults use electronic cigarettes (electronic nicotine delivery systems (ENDS)) for smoking cessation and reasons why they may or may not successfully quit smoking.

Methods
Longitudinal qualitative data were collected annually from 2017 to 2019 for 25 young adult tobacco users (aged 18–29 years) in California (USA) who used ENDS to quit/reduce smoking. Thematic and trajectory analyses were used to identify key within-person and between-person changes in tobacco/nicotine use over time.

Results

Five types of tobacco use transition were identified among baseline dual users of cigarettes and ENDS:
sustained dual use without reduced smoking
(n=8),
transition to exclusive daily ENDS use
(n=6),
sustained dual use with reduced smoking
(n=5),
transition back to exclusive smoking
(n=4) and
transition to neither smoking nor vaping
(n=2). Participants’ ENDS use behaviour varied over time in terms of vaping quantity and device characteristics (eg, changing nicotine concentrations/flavours, switching between multiple devices). Three themes that related to successfully replacing cigarettes with ENDS were
perceived positive physical effects, perceived satisfaction and enjoyment
and
context changes
. Four themes for unsuccessful replacement were
perceived negative physical discomforts, perceived addictiveness and harm
,
unsatisfactory substitution for cigarettes
and
device malfunction
.

Conclusions
Young adults’ experiences with using ENDS as a smoking cessation aid were highly variable. Adequate nicotine delivery and perceived safety and benefits contributed to successfully reducing or quitting cigarettes. Providing behavioural counselling and standardising ENDS products may enhance cessation for young adults.
The selective role of the orbital angular momentum on the reaction stereo-dynamics
Authors: Falcinelli Stefano , Parriani Marco , Vecchiocattivi Franco , Pirani Fernando 
Published: April 2023

AbstractThis paper reports on the characterization of the stereo-dynamic controlling three different chemi-ionization reactions, recent objective of our study, since they participate to the balance of phenomena occurring in plasma, interstellar medium, planetary atmospheres, flames and lasers. The optical potential, obtained by a phenomenological method and defined in the whole space of the relative configurations of reagents, has been formulated in an accurate and internally consistent way for three different systems. Some cuts of the multidimensional potential, that asymptotically correlate with a specific fine level of the open shell atom and/or with a defined orientation of the molecular reagent, have been exploited in the present study to emphasize crucial features of the collision dynamics along selected entrance channels of the reactions. Consistently, basic quantities determining the topology of the reaction stereo-dynamics have been properly defined, emphasizing in the three cases relevant changes in the microscopic reaction evolution. Much attention focused on the selectivity of the orbital angular momentum, affecting each collision event at any chosen collision energy. It controls the relative weight of two different reaction mechanisms. The direct reaction mechanism is driven by short-range chemical forces, promoting, by direct electron transfer between reagents, a prototypical elementary oxidation reaction. The indirect mechanism, controlled by the combination of long-range chemical and physical forces, can be triggered by a virtual photon exchanged between reagents, promoting a sort of photo-ionization process. Obtained results and emphasized differences appear to be of general interest for many other elementary processes, more difficult to characterize at this level of detail.Graphic abstract
Quantum quasi-Lie systems: properties and applications
Authors: Cariñena J.F. , de Lucas J. , Sardón C. 
Published: April 2023

AbstractA Lie system is a non-autonomous system of ordinary differential equations describing the integral curves of a t-dependent vector field that is equivalent to a t-dependent family of vector fields within a finite-dimensional Lie algebra of vector fields. Lie systems have been generalised in the literature to deal with t-dependent Schrödinger equations determined by a particular class of t-dependent Hamiltonian operators, the quantum Lie systems, and other systems of differential equations through the so-called quasi-Lie schemes. This work extends quasi-Lie schemes and quantum Lie systems to cope with t-dependent Schrödinger equations associated with the here-called quantum quasi-Lie systems. To illustrate our methods, we propose and study a quantum analogue of the classical nonlinear oscillator searched by Perelomov, and we analyse a quantum one-dimensional fluid in a trapping potential along with quantum t-dependent Smorodinsky–Winternitz oscillators.
Understanding the direct detection of charged particles with SiPMs
Authors: Carnesecchi F. , Vignola G. , Agrawal N. , Alici A. , Antonioli P. , Arcelli S. , Bellini F. , Cavazza D. , Cifarelli L. , Colocci M. , Durando S. , Ercolessi F. , Ficorella A. , Fraticelli C. , Garbini M. , Giacalone M. , Gola A. , Hatzifotiadou D. , Jacazio N. , Margotti A. , Malfattore G. , Nania R. , Noferini F. , Paternoster G. , Pinazza O. , Preghenella R. , Rath R. , Ricci R. , Rignanese L. , Rubini N. , Sabiu B. , Scapparone E. , Scioli G. , Strazzi S. , Tripathy S. , Zichichi A. 
Published: April 2023

AbstractIn this paper, evidence that the increased response of SiPM sensors to the passage of charged particles is related mainly to Cherenkov light produced in the protection layer is reported. The response and timing properties of sensors with different protection layers have been studied.
Kinome Reprogramming Is a Targetable Vulnerability in ESR1 Fusion-Driven Breast Cancer
Authors: Gou Xuxu , Kim Beom-Jun , Anurag Meenakshi , Lei Jonathan T. , Young Meggie N. , Holt Matthew V. , Fandino Diana , Vollert Craig T. , Singh Purba , Alzubi Mohammad A. , Malovannaya Anna , Dobrolecki Lacey E. , Lewis Michael T. , Li Shunqiang , Foulds Charles E. , Ellis Matthew J. 
Published: April 2023

Abstract

Transcriptionally active ESR1 fusions (ESR1-TAF) are a potent cause of breast cancer endocrine therapy (ET) resistance. ESR1-TAFs are not directly druggable because the C-terminal estrogen/anti-estrogen–binding domain is replaced with translocated in-frame partner gene sequences that confer constitutive transactivation. To discover alternative treatments, a mass spectrometry (MS)–based kinase inhibitor pulldown assay (KIPA) was deployed to identify druggable kinases that are upregulated by diverse ESR1-TAFs. Subsequent explorations of drug sensitivity validated RET kinase as a common therapeutic vulnerability despite remarkable ESR1-TAF C-terminal sequence and structural diversity. Organoids and xenografts from a pan-ET–resistant patient-derived xenograft model that harbors the ESR1-e6&gt;YAP1 TAF were concordantly inhibited by the selective RET inhibitor pralsetinib to a similar extent as the CDK4/6 inhibitor palbociclib. Together, these findings provide preclinical rationale for clinical evaluation of RET inhibition for the treatment of ESR1-TAF–driven ET-resistant breast cancer.

Significance:
Kinome analysis of ESR1 translocated and mutated breast tumors using drug bead-based mass spectrometry followed by drug-sensitivity studies nominates RET as a therapeutic target.
See related commentary by Wu and Subbiah, p. 3159
Abnormal Mechanics Relate to Myocardial Fibrosis and Ventricular Arrhythmias in Patients With Mitral Valve Prolapse
Authors: Nagata Yasufumi , Bertrand Philippe B. , Baliyan Vinit , Kochav Jonathan , Kagan Ruth D. , Ujka Kristian , Alfraidi Hassan , van Kampen Antonia , Morningstar Jordan E. , Dal-Bianco Jacob P. , Melnitchouk Serguei , Holmvang Godtfred , Borger Michael A. , Moore Reece , Hua Lanqi , Sultana Razia , Calle Pablo Villar , Yum Brian , Guerrero J. Luis , Neilan Tomas G. , Picard Michael H. , Kim Jiwon , Delling Francesca N. , Hung Judy , Norris Russell A. , Weinsaft Jonathan W. , Levine Robert A. 
Published: April 2023

Background:
The relation between ventricular arrhythmia and fibrosis in mitral valve prolapse (MVP) is reported, but underlying valve-induced mechanisms remain unknown. We evaluated the association between abnormal MVP-related mechanics and myocardial fibrosis, and their association with arrhythmia.

Methods:
We studied 113 patients with MVP with both echocardiogram and gadolinium cardiac magnetic resonance imaging for myocardial fibrosis. Two-dimensional and speckle-tracking echocardiography evaluated mitral regurgitation, superior leaflet and papillary muscle displacement with associated exaggerated basal myocardial systolic curling, and myocardial longitudinal strain. Follow-up assessed arrhythmic events (nonsustained or sustained ventricular tachycardia or ventricular fibrillation).

Results:

Myocardial fibrosis was observed in 43 patients with MVP, predominantly in the basal-midventricular inferior-lateral wall and papillary muscles. Patients with MVP with fibrosis had greater mitral regurgitation, prolapse, and superior papillary muscle displacement with basal curling and more impaired inferior-posterior basal strain than those without fibrosis (
P
<0.001). An abnormal strain pattern with distinct peaks pre–end-systole and post–end-systole in inferior-lateral wall was frequent in patients with fibrosis (81 versus 26%,
P
<0.001) but absent in patients without MVP with basal inferior-lateral wall fibrosis (n=20). During median follow-up of 1008 days, 36 of 87 patients with MVP with >6-month follow-up developed ventricular arrhythmias associated (univariable) with fibrosis, greater prolapse, mitral annular disjunction, and double-peak strain. In multivariable analysis, double-peak strain showed incremental risk of arrhythmia over fibrosis.

Conclusions:
Basal inferior-posterior myocardial fibrosis in MVP is associated with abnormal MVP-related myocardial mechanics, which are potentially associated with ventricular arrhythmia. These associations suggest pathophysiological links between MVP-related mechanical abnormalities and myocardial fibrosis, which also may relate to ventricular arrhythmia and offer potential imaging markers of increased arrhythmic risk.