bluuebunny/crossref_metadata_2025_split huggingface dataset:
PpMYB105 inhibited chilling injury by regulating PpMsrA1 in peach fruit
Authors: jiao GABA Caifeng , Jiao Caifeng , Dong Jing , Wei Yancheng 
Published: March 2023

Abstract
Cold storage can maintain the quality of postharvest fruit. However, peaches are easy to suffer from chilling injury during cold storage, leading to economic loss. Our preliminary research has demonstrated that MeJA supplementation reduced the chilling injury (CI) severity, and enhanced the methionine sulfoxide reductase A1 (PpMsrA1) expression. In this study, results showed that MeJA application elevated the MsrA activity and methionine (Met) content, and reduced the methionine-S-sulfoxide (Met-S-SO) content and reactive oxygen species (ROS) production. Moreover, PpMYB105, isolated by yeast one-hybrid screening, could activate the transcription of PpMsrA1 by binding to the MYB binding element in its promoter. The gene expression of PpMYB105 was up regulated by MeJA application. Overexpression of PpMYB105 in tomatoes enhanced the chilling tolerance and gene expression of SlMsrA1. Virus-induced gene silencing (VIGS) of PpMYB105 in peaches resulted in the increase in CI severity and the decrease in gene expression of PpMsrA1. Thus, PpMYB105 was involved in the MeJA-boosted chilling tolerance by regulating PpMsrA1.
Possible Molecular Explanation for the Resonance $Y(4500)$
Authors: süngü jale Y. , Veliev Elşen V. , Sundu Hayriye , Güngör Elif 
Published: March 2023

Abstract
The BESIII collaboration has discovered a new state with hidden charm-strange. Its mass is intriguingly close to the DsD̅s1 threshold and does not have the properties of the charmonium states. Working with the QCD sum rules (QCDSR) approach, we test if the charmonium-like structure Y (4500) , detected in the invariant mass spectrum K+ K-J/ψ may be interpreted as an exotic DsD̅s1 molecular structure with JPC=1--. Considering the contributions of QCD condensates up to operator dimension ten, we estimate the mass and decay constant of Y(4500) resonance. We get mY = (4488.35 ± 11.54) MeV in excellent agreement with the meson mass reported by BESIII and fY=(4.04 ± 0.36) ± 10-3 GeV4. Our findings indicate that a pseudoscalar-axialvector molecule current can well describe this state.
Application of human-computer interaction system based on machine learning algorithm in artistic visual communication
Authors: Nie Zexian , Yu Ying , Bao Yong 
Published: March 2023

Abstract
After entering the 21st century, with the development of science and technology represented by artificial intelligence, the content of art design and creation is increasingly rich. Its intelligence, interactivity, and digital content expression make the relationship between science and technology, art and people more close, and also bring new opportunities for the development of interactive art. Among them, human-computer interaction is more and more widely used. With the promotion of this kind of technology, interactive art is no longer based on auditory sensory experience, but on the in-depth study of human nature and comprehensive senses. On this basis, human-computer interaction technology is introduced, thus forming a comprehensive visual art form, which communicates and expresses through strong interaction, initiative and emotion. Based on machine learning technology and human-computer visual interaction technology, this paper explores the art visual communication module based on human-computer interaction system. By analyzing the audience's line of sight and visual differences, we can get the audience satisfaction, so as to improve the expression of the work. Firstly, this paper proposes the design of human-computer interaction visual structure, and introduces the basic structure of art visual communication system, the simulation of art visual scene, the process and evaluation index of human-computer interaction recognition, visual recognition effect, etc. based on the needs of visual interaction. Finally, it analyzes the common forms of human-computer interaction in art visual communication, the reconstruction of thinking mode of art visual communication and the development direction of art visual communication. This paper believes that human-computer interaction technology plays a leading role in the development of the art field. It has a variety of functions, display methods and values. By studying machine learning algorithm and human-computer interaction system, this paper applies it to the field of artistic vision, thus promoting the development of artistic visual communication.
Biodiversity Management within the Agroecosystem of the West Arsi Zone, Southeast Ethiopia
Authors: Babu Adugna , Hunderra Kitessa , Alemu Tibebu 
Published: March 2023

Abstract
The main objective of this study was to assess biodiversity management practices within the agroecosystem of the West Arsi Zone of Southeastern Ethiopia. Four Woredas namely, Wondo, Adaba, Kokossa and Nensebo were purposively selected based on their conservation and diversification practices. The Primary data collection methods like questionnaires, Focus group discussion, and key informant interview and field observation were used to identify the biodiversity conservation practices, especially, the vegetation diversity and the livestock management practices within the agroecosystem. Books, reports and articles were used as secondary sources of information to support the study. Diversity indices, Shannon diversity index and Evenness index were used to identify the vegetation diversity based on the three dominant land use practices homegarden, field crops and grazing land. To sum up, Least significance Difference (LSD) test at 0.05 was used to identify the vegetation diversity per land uses and households and also to check significant level among the household respondents. Therefore, SPSS (version 21) was implemented to assess the diversity of plants and animals within the agroecosystem of the study areas. The result of the study indicates that there was significant different among the Woredas (P < 0.05) regarding vegetation diversity per land uses and households. Based on the field experiments, homegarden is the most land use category having different diversity of vegetation (H׳=4.77) followed by field crops (H׳=4.06).From equity perspective, the homegarden vegetation of the agroecosystem is also the most evenly distributed (J׳=0.99) followed by grazing land (J׳=0.98).From the four Woredas, the highest vegetation diversity was recorded in Wondo Woreda, particularly, in homegarden (20.86 ± 3.85), while the lowest vegetation diversity was recorded in the homegarden of the Nensebo Woreda (7.38 ± 0.644). However, the Kokossa Woreda is the best in terms of vegetation diversity within the grazing land (13.774 ± 1.54) followed by the Nensebo Woreda (9.8723 ± 1.115). There is also significant variation (P < 0.005) among the Woredas regarding the livestock holding per households. For instance, Kokossa Woreda is the best in terms of cattle population per households (12.495 ± 4.633) followed by the Adaba Woreda (8.043 ± 2.86).Since the agroecosystem of the West Arsi Zone is full of animal and vegetation diversity, all concerned stakeholders should give due attention for the area to get greater output of production.
Early subchondral bone loss plays an important role in initiation and progression of osteoarthritis in the mouse DMM model
Authors: Hashimoto Teruaki , Akagi Masao , Hashimoto Kazuhiko , Morishita Takafumi , Tsukamoto Ichiro , Ito Tomohiko 
Published: March 2023

Abstract
Introduction:
Recently, uncoupled bone remodeling in the subchondral bone (SB) has been considered an important process to promote knee osteoarthritis (KOA). However, it is unclear how SB metabolism in the early stage of KOA affects the initiation and progression of the disease. This study aimed to investigate the relationship between the early changes in SB and the disease process, using wild-type (WT) mice and Tsukuba hypertensive mice (THM) with high-turnover bone metabolism.
Methods
Destabilization of the medial meniscus (DMM) or sham surgery was performed on the left knee of male 40-week-old THM and WT mice (n = 7 in each group). Bone volume/tissue volume (BV/TV), bone mineral density (BMD), and mean height (Hm, µm) in the medial tibial SB were longitudinally measured in vivo using micro-computed tomography (µCT) at 0, 1, 2, 4, 8, and 12 weeks postoperatively. Tibial cartilage degeneration was evaluated histologically using the OARSI score at 0, 1, 2, 4, 8, and 12 weeks postoperatively (n = 7 in each group).
Results
Significant decreases in BV/TV in both WT + DMM and THM + DMM groups were evident at 1 and 4 weeks postoperatively, respectively. Notably, significant decreases in the BMD (at 2 and 8 weeks postoperatively) and Hm (at 4 and 12 weeks postoperatively), and significant increases in the Osteoarthritis Research Society International (OARSI) score (at 2 and 8 weeks postoperatively) in the THM + DMM and WT + DMM groups were observed, respectively. A strong correlation was noted between the Hm and the BMD. These time-dependent changes in the BV/TV, BMD, Hm in the SB, and OARSI score were significantly enhanced in the THM + DMM group compared to the WT + DMM group.
Conclusions
These results indicate that SB loss at the early stage and the subsequent uncoupled bone remodeling, which can be enhanced by high-turnover osteoporosis, play an important role in the initiation and progression of KOA in the mouse DMM model. SB loss in the early stage could be a key target for preventing the initiation and progression of the disease.
Alpha-ketoglutaric acid mitigates the detrimental effects of soy antigenic protein on the intestinal health and growth performance of Mirror carp Cyprinus carpio
Authors: Zhou Zuliang , Zhao Jianghua , de Cruz Clement R. , Xu Hong , Wang Liansheng , Xu Qiyou 
Published: March 2023

Abstract
The study investigated the alleviated effects of Alpha-ketoglutaric acid (AKG) on the intestinal health of mirror carp (Cyprinus carpio Songpu) caused by soy antigenic protein. The dietswere formulated from fishmeal (CON),50% soybean meal (SBM),the mixture of glycinin and β-conglycinin (11+7S) and adding 1% AKG in the 11+7S (AKG). Carp (~4 g) in triplicate (30 fish per tank) was fed to apparent satiation thrice a day for six weeks. Compared with CON, SBM treatment resulted in significantly poor growth performance (P<0.05), whereas 11+7S and AKG treatments were not significantly different from CON (P>0.05). Gene expression of tumor necrosis factor (TNF-α) and interleukin-1 β (IL-1β) in proximal intestines (PI) and distal intestines (DI) were increased (P<0.05), and transforming growth factor (TGF-β) in PI and middle intestines (MI) was decreased (P<0.05) in both SBM and 11+7S. The gene expression of caspase-3 in DI increased in SBM (P<0.05) and the caspase-3 and caspase-9 in DI increased in 11+7S (P<0.05); conversely, the gene expression of TGF-β in PI and MI was increased, TNF-α and IL-1β in the MI, caspase-3, and caspase-9 in DI was decreased in AKG (P<0.05). mRNA levels of TOR in PI and MI, ACC in PI, MI and DI was decreased in SBM (P<0.05), the mRNA levels of AMPK in the PI and DI, TOR in PI, MI and DI, ACC in PI and DI, 4E-BP in DI was reduced in 11+7S (P<0.05). AMPK in the PI and DI, ACC in the PI and MI, TOR PI, MI, and DI, 4E-BP in PI and DI was recovered by AKG supplementation (P<0.05). Lipids and lipid-like metabolism, organic acids and derivatives metabolism increased in AKG dietary treatment. In conclusion, AKG reduces the expression of intestinal inflammation and apoptosis pathway and changes glycerophospholipid metabolism and sphingolipid metabolism in the intestine of fish.
Monitoring Malicious DNS Queries: An Experimental Case Study of Utilising the National Cyber Security Centre’s Protective DNS within a UK Public Sector Organisation
Authors: Carr Andrew , Alam Abu , Allison Jordan 
Published: March 2023

Abstract
The Domain Name System (DNS) is a key part of the modern inter-net but is also a route that malicious actors can use as cyber security attack. This paper reviews security and privacy additions to DNS, specifically Protective DNS (PDNS). We present a case study of the UK’s National Cyber Security Centre’s PDNS and its implementation in one regional public sector organisation in the north of England. We show how PDNS compliments DNS features to improve the security and privacy for eligible organisations. We also review how DNS queries have been blocked due to security risks. This block data can be integrated to a security information and event management system for further analysis and automation. The main contribution of this case study is that the NCSC’s PDNS Service is useful as part of a defense in depth strategy by allowing greater visibility of DNS queries and blocking of malicious content at the point of name resolution. This paper also recommends some techniques to improve the service which require further evaluation.
Monitoring roadkill in Spain: a three years survey during pandemic times
Authors: Antón Sergio Fuentes 
Published: March 2023

Abstract

Road-killed animal surveys are scarce in Spain compared to other countries. Also, the majority of the published papers about this issue, comprehends data analysis from particular species or involve only motorways or highways. This paper presents the monitoring of a common road during three years, from 1stJanuary 2020 to 31stDecember 2022 (both included), in order to evaluate wildlife loss by car hits. At the end of the survey, 53 individuals from 19 different species were found. The most affected groups were mammals with the 75% of car collisions and birds which obtained the 23% of total roadkills. Only one reptile was registered during the sampling. End of spring and summer were the seasons where wildlife was affected in high way, while autumn was the one with less fauna affection. The landscape across the road seems to influence on the biodiversity loss and according to conservation interests, most of the registered animals belongs to endangered or protected species, especially birds and reptiles. Also domestic animals like cats and dogs were directly affected by vehicle collisions. Annual results showed that roadkill data increased along the three years of survey, being 2020 the year with less incidences, probably due to the mobility restrictions established during the COVID-19 crisis.
Deciphering the role of a SINE-VNTR-Alu retrotransposon polymorphism as a biomarker of Parkinson’s disease progression
Authors: Fröhlich Alexander , Pfaff Abigail , Middlehurst Ben , Hughes Lauren , Bubb Vivien , Quinn John , Koks Sulev 
Published: March 2023

Abstract
SINE-VNTR-Alu (SVA) retrotransposons are transposable elements which represent a source of genetic variation. We previously demonstrated that the presence/absence of a human-specific SVA, termed SVA_67, correlated with the progression of Parkinson´s Disease (PD). In the present study, we demonstrate that SVA_67 acts as expression quantitative trait loci, thereby exhibiting a strong regulatory effect across the genome using whole genome and transcriptomic data from the Parkinson’s Progression Markers Initiative cohort. We further show that SVA_67 is polymorphic for its variable number tandem repeat domain which correlates with both regulatory properties in a luciferase reporter gene assay in vitro and differential expression of multiple genes in vivo. Additionally, this variation’s utility as a biomarker is reflected in a correlation with a number of PD progression markers. These experiments highlight the plethora of transcriptomic and phenotypic changes associated with SVA_67 polymorphism which should be considered when investigating the missing heritability of neurodegenerative diseases.
Research on Non-destructive Intelligence Detection of Tightness of Stator Slot Wedges of Large Hydro-generator
Authors: Xie Xiaoping , Zhang Chunhui , Mao Yedong , Cheng Shijie , Li Xuewei , Zhou Cong , Chen Weidong 
Published: March 2023

Abstract

In this study, the classification threshold of stator slot wedge tightness is established, and a non-destructive testing technology of stator slot wedge tightness based on acoustics is proposed. Firstly, the classification threshold of slot wedge tightness is determined. Then through the compression test of corrugated plate, the corresponding corrugated plate shape variable is obtained. The mechanical classification threshold is transformed into the deformation threshold of corrugated plate. Secondly, the tightness test platform of stator slot wedge is established and the tightness test is carried out. Then, the percussion signals is preprocessed by endpoint detection method, which greatly reduces the subsequent calculation. Subsequently, the characteristic parameters of the percussion signals are extracted by time-domain and frequency-domain analysis, and the characteristic parameters are screened by F-ratio method and Pearson correlation coefficient method. Finally, the Support Vector Machine(SVM) optimized by cross validation method is used to classify the test sets to realize pattern recognition. The results show that the tightness of stator slot wedge can be accurately detected by using this research. This research realizes the intelligent identification of the tightness of the stator slot wedge of the generator set, and provides an important reference for the tightness detection of the stator slot wedge.
Calibration method using a narrowband signal for measurement of the acoustic nonlinearity parameter
Authors: Song Dong-Gi , Park Seong-Hyun , Yoo Jiung , Lissenden Clifford J. , Jhang Kyung-Young 
Published: March 2023

Abstract
The measurement of absolute acoustic nonlinearity parameters using piezoelectric transducers consists of two steps; calibration and harmonic detection. In calibration, a frequency domain transfer function that converts the current output signal amplitude into the acoustic displacement amplitude is measured. Then, in harmonic detection, the amplitudes of the harmonics generated in the ultrasonic waves propagating through a medium are detected and converted to displacement amplitudes using the transfer function obtained in the calibration step for calculation of the acoustic nonlinearity parameters. However, in the conventional method, a broadband pulse signal is used for the calibration and a narrowband tone-burst signal is used for the harmonic detection; therefore, this method inconveniently uses different driving devices for calibration and harmonic detection. In this study, we propose a more convenient technique that can perform calibration using a narrowband signal so that a single driver of a narrowband signal can be used for both calibration and harmonic detection. To verify the validity of the proposed method, transfer functions were measured at typical frequencies for two types of material (aluminum alloy and fused silica) against two types of transducers (commercial PZT and LiNbO3 crystal), and the results were compared with those obtained using conventional calibration method. The transfer functions obtained by the conventional method and the proposed method showed good concordance, independent of material and transducer type.
Parent-adolescent informant discrepancies on the Strengths and Difficulties Questionnaire in the UK Millennium Cohort Study
Authors: Booth Charlotte , Moreno-Agostino Dario , Fitzsimons Emla 
Published: March 2023

Abstract
Background: Developmental researchers often use a multi-informant approach to measure adolescent behaviour and adjustment, but informant discrepancies are common. In general population samples, it is often found that parents report more positive and less negative outcomes compared to adolescents. The aim of this study was to investigate factors associated with informant discrepancies, including adolescent’s sex, and parent’s level of psychological distress and highest level of education.
Methods: Informant discrepancies on the Strengths and Difficulties Questionnaire were investigated using a Latent Difference Score (LDS) approach, which estimates the true difference between parent and adolescent reports in a structural equation model. The sample were parent-adolescent dyads from the seventh wave of the UK Millennium Cohort Study (N = 6,631, 49.1% female, aged 17 years).
Results: Compared to adolescents, parents reported lower levels of difficulties (emotional symptoms, peer problems, conduct problems) and higher levels of pro-social behaviour in their children. Conditional effects were found, as discrepancy was greater amongst parent-daughter dyads for emotion and peer problems, and greater amongst parent-son dyads for pro-social behaviour. Parent-adolescent discrepancy was also greater if parents had a lower level of psychological distress or a higher level of education.
Conclusions: In a large general population sample from the UK, it was found that adolescents tended to report more negative and less positive outcomes than parents reported about them. Discrepancy was conditional on various factors, including adolescent’s sex, and parent’s level of psychological distress and education, showing that specific informant biases are likely to impact the measurement of adolescent behaviour and adjustment.
“Less patch more growth?”: comparing two different schools of thought in aortic arch reconstruction techniques during Norwood I
Authors: Uzdenov Murat , Ballazhi Fatos , Kroll Johannes , Markova Irina , Zürn Christoph , Stiller Brigitte , Kubicki Rouven , Kari Fabian 
Published: March 2023

Abstract
Background: We compared two different philosophies at one institution with respect to arch-geometry and clinical results: no/minimal patch vs. abundant patch augmentation.
Methods: Single center retrospective analysis of the interstage angiographies of 82 patients: 41 (50%) underwent stage I Norwood-type palliation with minimal/no patch material for arch augmentation (Group 1, 2001-2011), and 41 (50%) with an abundant use of patch material (Group 2, 2011-2019). Aortic arch angle, distal arch:descending ratio, planimetry of whole arch, left hilar space were quantified. Clinical endpoints were all interstage interventions on the aortic arch, left pulmonary artery and readmissions due to left lung atelectasis and respiratory failure.
Results: Arch angles were more acute in Group 1 (81°±13,2 vs. 90°±10,28, P = .012), with no difference in left hilar space (0,28±0,08 vs. 0,29±0,06, P = .43). Distal arch:descending ratio was smaller in Group 1 (0,72±0,2 vs 0,86±0,19, P = .04). Interstage interventions on the aortic arch and descending aorta were provided in 17 (41,5%) patients in Group 1 versus 19 (46,3%) patients in Group 2. Transcatheter interventions on the left pulmonary artery were necessary in 18 Group 1 patients versus 18 Group 2 patients (43,9%). Unilateral left lung atelectasis and respiratory failure occurred in 2 patients (4,4%) in Group 1 and in 3 patients (7,3%) in Group 2.
Conclusions: Abundant patch material is associated with less acute aortic arch angles and leads to significantly better distal arch growth. However, this theoretical geometric benefit did not lead to greater left hilar space, nor did it prove to be superior with respect to interstage-interventions on left hilar structures. Mid-term no clinical effect was detected but future long-term effects are substantial.