bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel functional insights into the microbiome inhabiting marine plastic debris: critical considerations to counteract the challenges of thin biofilms using multi-omics and comparative metaproteomics.
Authors: Messer Lauren F. , Lee Charlotte E. , Wattiez Ruddy , Matallana-Surget Sabine 
Published: June 2023

Abstract
Background
Microbial functioning on marine plastic surfaces has been poorly documented, with only two studies using metaproteomics to unravel microbial genotype-phenotype linkages in the marine ‘plastisphere’. Here we provide a comprehensive methodological assessment for plastisphere metaproteomics, using multi-omics and data mining on thin plastic biofilms from an understudied cold environment, to provide unique insights into plastisphere metabolism. Our robust experimental design assessed DNA/protein co-extraction and cell lysis strategies, proteomics workflows, and diverse protein search databases, to encourage the more widespread application of these techniques to resolve plastisphere function.
Results
For the first time, we demonstrate the predominance and activity of hydrocarbonoclastic genera (Psychrobacter, Flavobacterium, Pseudomonas) within a primarily heterotrophic plastisphere. Correspondingly, oxidative phosphorylation, the citrate cycle, and carbohydrate metabolism were the dominant pathways expressed. We also identified quorum sensing and toxin-associated proteins in Streptomyces, stress response proteins expressed by Psychrobacter, Planococcus and Pseudoalteromonas, and xenobiotics degradation proteins in Psychrobacter and Pseudoalteromonas. Interestingly, a targeted search strategy identified plastic biodegradation enzymes, such as polyamidase, hydrolase, and depolymerase, expressed by rare taxa. In contrast to previous research, pathogenic genera were active, expressing virulence factors and mechanisms of antimicrobial resistance.
Conclusion
Our study demonstrates the power of multi-omics and comparative metaproteomics to resolve plastisphere functioning, to provide new bioengineering perspectives and improved assessment of the risks of plastic pollution.
Unleashing the Power of Evolutionary Machine Learning in CBIR: A Study of Kernelized Radial Basis Auto-Encoder Function Neural Network
Authors: Bose Anandh Sam Chandra , Singh Laxman , Qamar Shamimul , Uma S , Annabel L. Sherly Puspha , Singla Sanjay 
Published: June 2023

Abstract
The goal of Content-Based Image Retrieval (CBIR) is to compare a query image with similar images from a large dataset. Typically, the ranking of retrieved photos is based on how similar the representative features of the query image and the dataset images are. Machine learning (ML) methods have been investigated as a practical approach to decrease the semantic gap. This research proposes a novel technique that utilizes evolutionary machine learning in CBIR. The input image is processed and classified using a Kernelized Radial Basis Auto-Encoder Function Neural Network (Ker_RadBAEFNN). Then, the input neural network is optimized using reinforcement in CBIR. Experimental analysis is carried out in terms of accuracy, precision, recall, F-1 score, RMSE, and MAP for various input datasets. The suggested architecture has exceptional performance in feature learning without prior knowledge of the images, categorization, and optimization. The proposed method achieved 98% accuracy, 96% precision, 79% recall, a 66% F-1 score, a 69% RMSE, and a 59% MAP.
DIGITAL ISLAMIC NETWORK: BUILDING EXCELLENT SERVICE FOR CUSTOMERS AT BANK MUAMALAT TULUNGAGUNG IN THE DIGITAL ERA
Authors: Cahyaningtyas Ekalaka Febian , Fauza Nilna 
Published: June 2023

Abstrak: Perkembangan teknologi pada era ini menunjukkan perkembangan yang begitu pesat. Dunia mengalami kemajuan luar biasa dalam teknologi dan inovasi. Kehadiran aplikasi Digital Islamic Network (DIN) sebagai platform digital yang bermanfaat disambut antusias masyarakat. Untuk itu penelitian ini bertujuan untuk mengetahui peranan aplikasi Digital Islamic Network (DIN) dalam meningkatkan pelayanan nasabah di Bank Muamalat KCP Tulungagung. Artikel ini menggunakan metode deskriptif-kualitatif dengan pendekatan penelitian lapangan dan penelitian kepustakaan. Sumber data yang digunakan dalam penelitian ini adalah sumber data primer dan sumber data sekunder. Hasil kajian menunjukkan bahwa Digital Islamic Network (DIN) di Bank Muamalat KCP Tulungagung berperan untuk memudahkan nasabah mendapatkan layanan yang berkualitas dan profesional dengan mewujudkan efektifitas dan efisiensi layanan perbankan kepada nasabah. Hal ini dibuktikan dengan peran Muamalat Digital Islamic Network (DIN) dalam meningkatkan kualitas pelayanan, memberikan kemudahan pelayanan kepada pelanggan, efisiensi waktu, memberikan jaminan keamanan bagi pelanggan, dan memberikan kepuasan pelayanan.
Abstract: The development of technology in the current era show growing rapidly. The world is experiencing tremendous progress in technology and innovation. The presence of the Digital Islamic Network (DIN) application as a beneficial digital platform is enthusiastically welcomed by the public. For this reason, this study aims to determine the role of Digital Islamic Network (DIN) applications in improving customer service at Bank Muamalat KCP Tulungagung. This article uses a descriptive-qualitative method with a field research and library research approach. Sources of data used in this study are primary data sources and secondary data sources. The result of the study shows that the Digital Islamic Network (DIN) at Bank Muamalat KCP Tulungagung plays a role in making it easy for customers to receive quality and professional services by realizing the effectiveness and efficiency of banking services to customers. This is proven by the role of Muamalat Digital Islamic Network (DIN) in improving the quality of service, providing easy service to customers, time efficiency, providing security guarantees for customers, and providing service satisfaction.
ANALISI PERBANDINGAN PENGGUNAAN BANDWIDTH APLIKASI CONFERENSI ONLINE PADA WEBEX, GOOGLE MEET DAN ZOOM
Authors: Muran Riko Gusjakal , Lukito Yuan , Haryono Nugroho Agus 
Published: June 2023

During this pandemic, conference calls or often called concall became a tool that was often used due to government regulations regarding the new COVID-19. Due to these regulations the concall application is commonly used by all agencies. Because of this, new users of concall applications haveincreased and many of these new users still do not understand which applications are suitable for the network in their area. Therefore, researchers made research to provide information about QoS (Quality Of Service) from several applications, namely Google Meet, Webex, and Zoom. In thisstudy, researchers conducted research with 20 PC clients who were given network boundaries of 0%, 25%, 50%, and 75% using the Queue Tree andPCQ methods.
In the study, there were 3 stages in the process, namely preparation, data collection, and data processing. The variables used by researchers areDelay, Jitter, Packet Loss, and Throughput. These variables are retrieved using the Wireshark application on the client pc. In this study, researchers used the Queue Tree and PCQ methods to provide dynamic limits to the user and to be able to limit accordingly for each application hierarchically.
In this study, it is known that some applications have a very significant decrease in performance when the confined network reaches 50% or more of the total network obtained by the researcher when conducting the research. Thus the researchers hope that with this study readers can get information about which applications are good for use in their respective areas which have different speeds.
Improving soil moisture content to increase strawberry growth indicators using the hydroponic method
Authors: Cao Huijuan , Han Yongguang , Peng Jing , Peng Xiaorui 
Published: June 2023

Abstract

Water and soil moisture management are very important factors in strawberry cultivation, and they directly affect strawberry growth and yield. Full moisture and suitable soil moisture can promote strawberry growth and grow faster and higher; proper soil moisture can maintain soil nutrients and prevent soil loss, thus maintaining soil fertility. In addition, adequate moisture and suitable soil moisture can make the strawberry grow more healthily, thus improving its quality and reducing the occurrence of hollow fruit. Therefore, in strawberry cultivation, reasonable water and soil moisture management is very important, growers need to carry out timely irrigation and drainage according to the growth of strawberry, to ensure the growth and yield of strawberry. Its disadvantages include the following: vulnerable to diseases and pests, high requirements for climatic conditions, high requirements for climatic conditions and high production costs. As a new agricultural technology, hydroponics can save land resources and reduce land pollution by cultivating plants in containers instead of planting plants on the land.
A quantitative and qualitative investigation of ball and foam covers coated with cement slurry and their composition on reducing evaporation and choosing the best coating by the AHP method
Authors: Karami Hojat , Sobhi Alireza , Vagheei Ramazan 
Published: June 2023

Abstract

The present study sought to use a 20 mm thick foam coated with cement slurry and blue ball in 50 and 80% surface covers, and their composite in 80% surface coverage in the Colorado pan. Then, the pans' daily evaporation was calculated and compared with the amount of control evaporation. The results indicated that the combined method (plastic ball and foam) with 80% reduced the evaporation by about 68%, plastic ball with 80% cover about 58%, foam with 80% coating about 53%, plastic ball with 50% coating about 34%, and finally 50% foam by about 28%. Concerning the statistical analysis of this research, a significant correlation was observed between the control pan and average temperature, although there was no fixed and uniform relationship between the control pan and maximum wind speed, air pressure, average relative humidity, and the number of sunshine hours. In this research, foam 50%, composite 80%, foam 80%, ball 80%, and ball 50% had better performance in terms of the best water quality based on the water electrical conductivity (EC), respectively. Finally, the composite cover 80% was selected as the best coverage using the analytic hierarchy process (AHP) and considering different criteria.
Synthesis and Anticancer Activity of Novel Indole Derivatives as Dual
EGFR/SRC Kinase Inhibitors
Authors: Olgen Sureyya , Kaleli Sevde Nur Biltekin , Karaca Banu Taktak , Demirel Ural U. , Bristow Hacer Karatas 
Published: June 2023

Background:
Recent studies showed that the cooperation between c-SRC and EGFR is responsible
for more aggressive phenotype in diverse tumors, including glioblastomas and carcinomas of the
colon, breast, and lung. Studies show that combination of SRC and EGFR inhibitors can induce apoptosis
and delay the acquired resistance to chemotherapy. Therefore, such combination may lead to a new
therapeutic strategy for the treatment of EGFR-mutant lung cancer. Osimertinib was developed as a
third-generation EGFR-TKI to combat the toxicity of EGFR mutant inhibitors. Due to the resistance and
adverse reaction of osimertinib and other kinase inhibitors, 12 novel compounds structurally similar to
osimertinib were designed and synthesized.

Methods:
Compounds were synthesized by developing novel original synthesis methods and receptor interactions
were evaluated through a molecular docking study. To evaluate their inhibitory activities
against EGFR and SRC kinase, in vitro enzyme assays were used. Anticancer potencies were determined
using lung, breast, prostate (A549, MCF6, PC3) cancer cell lines. Compounds were also tested against
normal (HEK293) cell line to evaluate their cyctotoxic effects.

Results:
Although, none of compounds showed stronger inhibition compared to osimertinib in the EGFR
enzyme inhibition studies, compound 16 showed the highest efficacy with an IC50 of 1.026 μM. It also
presented potent activity against SRC kinase with an IC50 of 0.002 μM. Among the tested compounds,
the urea containing derivatives 6-11 exhibited a strong inhibition profile (80.12-89.68%) against SRC kinase
in comparison to the reference compound dasatinib (93.26%). Most of the compounds caused more
than 50% of cell death in breast, lung and prostate cancer cell lines and weak toxicity for normal cells in
comparison to reference compounds osimertinib, dasatinib and cisplatin. Compound 16 showed strong
cytotoxicity on lung and prostate cancer cells. Treatment of prostate cancer cell lines with the most active
compound, 16, significantly increased the caspase-3 (8-fold), caspase-8 (6-fold) and Bax (5.7-fold)
levels and decreased the Bcl-2 level (2.3-fold) compared to the control group. These findings revealed
that the compound 16 strongly induces apoptosis in the prostate cancer cell lines.

Conclusion:
Overall kinase inhibition, cytotoxicity and apoptosis assays presented that compound 16
has dual inhibitory activity against SRC and EGFR kinases while maintaining low toxicity against normal
cells. Other compounds also showed considerable activity profiles in kinase and cell culture assays.
The Marine Factor 3,5-dihydroxy-4-methoxybenzyl Alcohol
Suppresses Cell Growth, Inflammatory Cytokine Production, and
NF-κB Signaling-enhanced Osteoclastogenesis in In vitro Mouse
Macrophages RAW264.7 Cells
Authors: Yamaguchi Masayoshi , Yoshiike Kenji , Watanabe Hideaki , Watanabe Mitsugu 
Published: June 2023

Background and Objective:
The novel marine factor 3,5-dihydroxy-4-
methoxybenzyl alcohol (DHMBA) was originally identified in the Pacific oyster
Crassostrea Gigas. DHMBA has been shown to prevent oxidative stress by scavenging
radicals and enhance the production of antioxidant proteins. However, the
pharmacologic role of DHMBA has been poorly understood. Inflammation is implicated in
the pathogenesis of many diseases. Inflammatory cytokines are produced in macrophages
with stimulation of lipopolysaccharide (LPS) and are used as biomarkers that
cause diverse disease conditions. Therefore, this study has been undertaken to
elucidate whether DHMBA expresses anti-inflammatory effects in in vitro mouse
macrophage RAW264.7 cells.

Methods:
Mouse macrophage RAW264.7 cells were cultured in a medium containing
10% fetal bovine serum (FBS) with or without DHMBA (1-1000 μM).

Results:
Culturing with DHMBA (1-1000 μM) suppressed the growth and stimulated the
death of RAW264.7 cells in vitro, leading to a decrease in cell number. Treatment with
DHMBA reduced the levels of Ras, PI3K, Akt, MAPK, phospho-MAPK, and mTOR,
which are signalling factors to promote cell proliferation, and it raised the levels of p53,
p21, Rb, and regucalcin, which are cell growth suppressors. DHMBA treatment elevated
caspase-3 and cleaved caspase-3 levels. Interestingly, DHMBA treatment repressed the
production of inflammatory cytokines, including tumor necrosis factor-α, interleukin-6,
interleukin-1β, or prostaglandin E2, which were enhanced by LPS stimulation. Notably,
the levels of NF-κB p65 were increased by LPS treatment, and this augmentation was
repres-sed by DHMBA treatment. Moreover, LPS treatment stimulated osteoclastogenesis
of RAW264.7 cells. This stimulation was blocked by DHMBA treatment, and this
effect was not caused by the presence of an NF-κB signalling inhibitor.

Conclusion:
DHMBA was found to potentially suppress the activity of inflammatory
macrophages in vitro, suggesting its therapeutic usefulness in inflammatory conditions.
Synthesis and Chiral Separation of Some New Derivatives of Imidazo [1, 2-
a] Pyridine
Authors: Keltoum Cheham Bouler Bag Oum , El Amin Zaid Mohamed , Nasser Belboukhari , Khaled Sekkoum , Hassan Aboul-Enein 
Published: June 2023

Introduction:
Mannich base is the result of the three-part chemical reaction known as the
Mannich reaction, which includes the amino alkylation of an acidic proton adjacent to a carbonyl group
by formaldehyde and either a primary or secondary amine.

Method::
In a 1L flask, dissolve 28.2 g (0.3 mol) of 2-aminopyridine, and 400mL of ethanol,
then add 32.6g (0.3 mol) of chloroacetone and put 30 drops of acetic acid. The organic phases are combined
and dried with Magnesium sulfate. After solvent evaporation, we have recovered our product which
is a 2-methylimidazo [1, 2-a] pyridine product.

Result:
A C-18 bonded stationary phase provided one significant peak for all of the compounds under
examination providing solid proof of the compound purity. This was used to demonstrate the purity of the
compounds. Then, the potential enantiomers of these chiral compounds were obtained using the four
CSPs.

Conclusion:
In this study, several novel Mannich bases were prepared. These bases had rendement ranging
from 76 to 94.73, and their chiral separation was discussed utilizing four polysaccharide-based CSPs:
ChiralpakAS, Chiralcel OD, Chirapak®AS-3R, and Chiralcel OJ.
Therapeutic Potential of Indian Medicinal Herbs and Current Therapeutic
Approach used to Mitigate the Symptoms of Chronic Fatigue Syndrome:
A Review
Authors: Singh Nisha , Sharma Rahul Kumar , Kushwah Ajay Singh , Kumar Manish 
Published: June 2023

Abstract:
Chronic fatigue syndrome (CFS) is a complex condition marked by severe exhaustion
that lasts at least 6 months. The global prevalence of CFS ranging between 0.4% and 2.5% is growing.
Women are affected by CFS more often than men. It is considered a common condition in developed
countries. There is no approved treatment for CFS but symptoms can be managed and controlled
persistent exhaustion causes significant impairment in daily routine activities. Lowered
ATP synthesis, mitochondrial impairment, decreased oxidative phosphorylation, disruption of the
hypothalamic–pituitary–adrenal (HPA) axis, and an imbalance of brain neurotransmitters play a
major role in the pathophysiology of CFS. The purpose of the present study is to figure out the several
plants that are used as a source of medication in chronic fatigue syndrome (CFS) and its current
therapeutic approach. The Indian medicinal herbs described in this article are very efficacious
in the management of chronic fatigue syndrome symptoms due to the presence of phytochemicals.
This review article also covers the current therapeutic approach for chronic fatigue syndrome in a
concise form that comprises CBT (Cognitive based therapy), GET (Graded exercise therapy), usage
of immunoglobins, psychodynamic counseling, and yoga therapy that includes isometric yoga and
yoga nidra are very beneficial in alleviating the symptoms of chronic fatigue syndrome. Antidepressants,
immunomodulatory agents, and corticosteroids come under conventional medication for CFS.
This article explores different Indian medicinal herbs, their pharmacological properties, and their potential
role and current treatments for reducing the severity of symptoms of chronic fatigue syndrome.
Analisis Dampak Pelatihan Peningkatan Kompetensi Layanan Konseling Kelompok pada Guru BK SMA Se-Kabupaten Sleman
Authors: Aminah Siti , Purnama Diana Septi , Suwarjo Suwarjo , Rahman Fathur 
Published: June 2023

Abstrak: Kompetensi profesional guru bimbingan dan konseling merupakan aspek penting yang membekali guru bimbingan dan konseling dalam memberikan bimbingan dan konseling di sekolah. Salah satu kompetensi yang perlu ditingkatkan yaitu kemampuan guru bimbingan dan konseling dalam menyelenggarakan layanan konseling kelompok. Metode yang digunakan dalam pelatihan ini adalah ceramah, diskusi, penugasan dan simulasi. Hasil analisis dampak pelatihan peningkatan kompetensi layanan konseling kelompok dengan analisis kualitatif menunjukkan bahwa Guru bimbingan dan konseling memiliki minat yang tinggi dalam mengikuti kegiatan. Berdasarkan analisis penugasan ditemukan bahwa terdapat peningkatan pemahaman dan keterampilan dalam merancang layanan konseling kelompok. Hasil analisis observasi dan wawancara setelah simulasi menunjukkan adanya peningkatan keterampilan dalam melaksanakan layanan konseling kelompok. Hasil analisis kuesioner evaluasi berkaitan tentang penyelenggaraan pelatihan menunjukkan bahwa pelatihan peserta merasa puas dan senang mengikuti pelatihan. Peserta mengharapkan adanya kegiatan lanjutan untuk memperdalam kemampuan menyelenggarakan layanan bimbingan dan konseling. Analysis of the Impact of Training for Competency Improvement in Group Counseling Services for High School Counseling Teachers in Sleman RegencyAbstract: The professional competence of guidance and counseling teachers is an important aspect that equips guidance and counseling teachers in providing guidance and counseling in schools. One of the competencies that need to be improved is the ability of guidance and counseling teachers in providing group counseling services. The methods used in this training are lectures, discussions, assignments and simulations. The results of the analysis of the impact of training on improving the competence of group counseling services with qualitative analysis indicate that guidance and counseling teachers have a high interest in participating in activities. Based on the assignment analysis, it was found that there was an increase in understanding and skills in designing group counseling services. The results of the analysis of observations and interviews after the simulation showed an increase in skills in implementing group counseling services. The results of the analysis of the evaluation questionnaire related to the implementation of the training show that the training participants were satisfied and happy to participate in the training. Participants expect further activities to deepen their ability to provide guidance and counseling services.