bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pot Marigold (Calendula officinalis) Powder in Rainbow Trout (Oncorhynchus mykiss) Feed: Effects on Growth, Immunity, and Yersinia ruckeri Resistance
Authors: Ghafarifarsani Hamed , Hoseinifar Seyed Hossein , Molayemraftar Taravat , Raeeszadeh Mahdieh , Van Doan Hien 
Published: May 2023

The current research investigated the effects of pot marigold (Calendula officinalis) powder on growth, biochemical parameters, digestive enzymes, serum and mucus immune responses, antioxidant defense, and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Fish (No. 450,

15.06
±
0.11

g;

mean
±
SE

) were randomly distributed to five groups fed with a diet containing 0 (control group), 0.5%, 1%, 1.5%, and 2% of pot marigold powder (MP) for 60 days. Then, fish were challenged with Y. ruckeri infection. Specific growth rate (SGR), weight gain (WG), final weight (FW), feed conversion ratio (FCR), mucus lysozyme (LYZ), mucus protease, serum nitroblue tetrazolium test (NBT), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) values in the 1%, 1.5%, and 2% MP groups significantly increased (

P
<
0.05

) compared to the other groups. Regression analysis exhibited that rainbow trout growth indices were polynomially linked to dietary MP concentrations. In this regard, the optimal levels of MP, according to growth parameters (SGR and FCR), were 1.31 and 1.4% diet, respectively. The intestinal protease, serum complement component 3 (C3), complement component 4 (C4), and LYZ activities in fish fed with the 1% and 1.5% MP-supplemented regime were higher (

P
<
0.05

) compared to the other groups. Also, fish fed with diets containing 1.5% MP had significantly higher intestinal lipase and mucus ALP activities than the other groups (

P
<
0.05

). Aspartate aminotransferase (AST) activity in all MP groups remarkably increased (

P
<
0.05

) compared to the control. Moreover, fish fed with a diet containing 1.5% MP had remarkably higher (

P
<
0.05

) lactate dehydrogenase (LDH) and total protein (TP) values than the other groups. Serum myeloperoxidase (MPO), total immunoglobulin (Ig), and mucus peroxidase values in the 1% MP group remarkably increased (

P
<
0.05

) compared to the other groups. In addition, catalase (CAT) and superoxide dismutase (SOD) activities in the 1.5% and 2% MP groups were significantly higher (

P
<
0.05

) compared to the others. However, malondialdehyde (MDA) levels in fish fed with the 1%, 1.5%, and 2% MP-supplemented diet remarkably decreased than in the other groups (

P
<
0.05

). The highest survival rate after a challenge with Y. ruckeri was recorded in the 1.5% group compared to the control group. Our findings revealed that using pot marigold powder in diets, especially at the 1.5% level, has positive effects on growth, digestive enzyme activities, antioxidant capacity, immune response, and disease resistance in O. mykiss.
APR-246 Enhances Colorectal Cancer Sensitivity to Radiotherapy
Authors: Xie Xuqin , Fan Chuanwen , Luo Bin , Zhang Jing , Jensen Lasse D. , Burman Jonas , Jönsson Carolin , Ljusberg Anna , Larsson Peter , Zhao Zengren , Sun Xiao-Feng 
Published: May 2023

Abstract
p53 mutation is common and highly related to radiotherapy resistance in rectal cancer. APR-246, as a small molecule, can restore the tumor-suppressor function to mutant p53. As there is currently no existing study on combining APR-246 with radiation in rectal cancer, our objective was to investigate whether APR-246 could enhance the sensitivity of colorectal cancer cells, regardless of their p53 status, to radiation treatment. The combination treatment had synergistic effects on HCT116p53-R248W/− (p53Mut) cells, followed by HCT116p53+/+ [wild-type p53 (p53WT)] cells, and exhibited an additive effect on HCT116p53−/− (p53Null) cells through inhibiting proliferation, enhancing reactive oxygen species, and apoptosis. The results were confirmed in zebrafish xenografts. Mechanistically, p53Mut and p53WT cells shared more activated pathways and differentially expressed genes following the combination treatment, compared with p53Null cells, although the combination treatment regulated individual pathways in the different cell lines. APR-246 mediated radiosensitization effects through p53-dependent and -independent ways. The results may provide evidence for a clinical trial of the combination in patients with rectal cancer.
CD69 Imposes Tumor-Specific CD8+ T-cell Fate in Tumor-Draining Lymph Nodes
Authors: Koyama-Nasu Ryo , Kimura Motoko Y. , Kiuchi Masahiro , Aoki Ami , Wang Yangsong , Mita Yukiyoshi , Hasegawa Ichita , Endo Yukihiro , Onodera Atsushi , Hirahara Kiyoshi , Motohashi Shinichiro , Nakayama Toshinori 
Published: May 2023

Abstract
Tumor-specific CD8+ T cells play a pivotal role in antitumor immunity and are a key target of immunotherapeutic approaches. Intratumoral CD8+ T cells are heterogeneous; Tcf1+ stemlike CD8+ T cells give rise to their cytotoxic progeny—Tim-3+ terminally differentiated CD8+ T cells. However, where and how this differentiation process occurs has not been elucidated. We herein show that terminally differentiated CD8+ T cells can be generated within tumor-draining lymph nodes (TDLN) and that CD69 expression on tumor-specific CD8+ T cells controls its differentiation process through regulating the expression of the transcription factor TOX. In TDLNs, CD69 deficiency diminished TOX expression in tumor-specific CD8+ T cells, and consequently promoted generation of functional terminally differentiated CD8+ T cells. Anti-CD69 administration promoted the generation of terminally differentiated CD8+ T cells, and the combined use of anti-CD69 and anti–programmed cell death protein 1 (PD-1) showed an efficient antitumor effect. Thus, CD69 is an attractive target for cancer immunotherapy that synergizes with immune checkpoint blockade.
Grounding the Global: Pathways to Elucidating Tensions in Chinese Contemporary Art
Authors: Mak Kathy Yim King 
Published: May 2023

Abstract
This article reviews two recent books on Chinese contemporary art, Sasha Welland's Experimental Beijing (2018) and Jenny Lin's Above Sea (2019), concerned with the sociopolitical contexts of the 1990s–2000s' globalizing Beijing and Shanghai respectively. By examining the two authors' respective methodologies—Welland's ethnographical field research and Lin's urban cultural research—, this article interprets how these two books shed light on the role of tensions in the intersectional global-local spaces of Chinese contemporary art. It argues that this field of art history necessitates the employment of non-art historical methodologies, as shown by the two books, in order to locate and make visible the intangible tensions hovering in the art's global-local spaces.
Commitment to Active Allyship Is Required to Address the Lack of Hispanic and Latinx Representation in the Earth and Atmospheric Sciences
Authors: Morales Annareli , Molina Maria J. , Trujillo-Falcón Joseph E. , Nuñez Ocasio Kelly M. , Lang Andrea L. , Murillo Elisa , Bieri Carolina , Barrett Bradford S. , Avilés Lourdes B. , Camargo Suzana J. 
Published: May 2023

Abstract
In 2021, people of Hispanic and Latinx origin made up 6% of the atmospheric and Earth sciences workforce of the United States, yet they represent 20% of the population. Motivated by this disparity in Hispanic and Latinx representation in the atmospheric and Earth science workforce, this manuscript documents the lack of representation through existing limited demographic data. The analysis presents a clear gap in participation by Hispanic and Latinx people in academic settings, with a widening gap through each education and career stage. Several factors and challenges impacting the representation disparity include the lack of funding for and collaboration with Hispanic-serving institutions, limited opportunities due to immigration status, and limited support for international research collaborations. We highlight the need for actionable steps to address the lack of representation and provide targeted recommendations to federal funding agencies, educational institutions, faculty, and potential employers. While we wait for systemic cultural change from our scientific institutions, grassroots initiatives like those proudly led by the AMS Committee for Hispanic and Latinx Advancement will emerge to address the needs of the Hispanic and Latinx scientific and broader community. We briefly highlight some of those achievements. Lasting cultural change can only happen if our leaders are active allies in the creation of a more diverse, equitable, and inclusive future. Alongside our active allies we will continue to champion for change in our weather, water, and climate enterprise.
Improving the Representation of Hail in the Thompson Microphysics Scheme
Authors: Jensen Anders A. , Thompson Gregory , Ikeda Kyoko , Tessendorf Sarah A. 
Published: May 2023

Abstract
Methods to improve the representation of hail in the Thompson–Eidhammer microphysics scheme are explored. A new two-moment and predicted density graupel category is implemented into the Thompson–Eidhammer scheme. Additionally, the one-moment graupel category’s intercept parameter is modified, based on hail observations, to shift the properties of the graupel category to become more hail-like since the category is designed to represent both graupel and hail. Finally, methods to diagnose maximum expected hail size at the surface and aloft are implemented. The original Thompson–Eidhammer version, the newly implemented two-moment and predicted density graupel version, and the modified (to be more hail-like) one-moment version are evaluated using a case that occurred during the Plains Elevated Convection at Night (PECAN) field campaign, during which hail-producing storms merged into a strong mesoscale convective system. The three versions of the scheme are evaluated for their ability to predict hail sizes compared to observed hail sizes from storm reports and estimated from radar, their ability to predict radar reflectivity signatures at various altitudes, and their ability to predict cold-pool features like temperature and wind speed. One key benefit of using the two-moment and predicted density graupel category is that the simulated reflectivity values in the upper levels of discrete storms are clearly improved. This improvement coincides with a significant reduction in the areal extent of graupel aloft, also seen when using the updated one-moment scheme. The two-moment and predicted density graupel scheme is also better able to predict a wide variety of hail sizes at the surface, including large (>2-in. diameter) hail that was observed during this case.
Evaluation of Probabilistic Snow Forecasts for Winter Weather Operations at Intermountain West Airports
Authors: Uden Dana M. , Wandishin Matthew S. , Schlatter Paul , Kraus Michael 
Published: May 2023

Abstract
This work set out to assess the performance of four forecast systems [the Short-Range Ensemble Forecast (SREF), High-Resolution Rapid Refresh Ensemble (HRRRE), the National Blend of Models (NBM), and the Probabilistic Snow Accumulation product (PSA) from the National Weather Service (NWS) Boulder, Colorado, Weather Forecast Office] when predicting snowfall events around the Intermountain West to advise winter weather decision-making processes at Denver International Airport. The goal was to provide airport personnel and the Boulder NWS Forecast Office with operationally relevant verification results on the timing and severity of these events so they are able to make better-informed decisions to minimize negative impacts of storms. Forecasts of snow events using various probability thresholds and a climatological snow-to-liquid ratio of 15:1 were evaluated against Meteorological Aerodrome Reports (METARs) for 24-h periods following four decision-making times spaced equally throughout the day. For the ensembles, a frequentist approach was used: the forecast probability equaled the percentage of ensemble members that predicted a snow event. The results show that the NBM had the best timing of snow events out of the products, while all the products tended to overforecast snow amount. Additionally, NBM had fewer snow events and rarely had high probabilities of snow, unlike the other forecast products.
Transdiagnostic therapy compared to progressive muscle relaxation on the emotional health of mothers with premature infants: A randomized controlled trial
Authors: Reisi Sajjad , Ahmadi Seyed Mojtaba , Foroughi Aliakbar , Bahrami Reza , Parvizifard Aliakbar 
Published: May 2023

Objective The aim of this study was to compare the effects of transdiagnostic therapy compared to progressive muscle relaxation (PMR) on emotion regulation strategies, self-compassion, maternal role adaptation, and social and work adjustment in mothers of premature infants. Materials and methods This was a randomized controlled trial with two groups and pre-test, post-test, and a two-month follow-up assessment. Included were 27 mothers randomly assigned to either the transdiagnostic therapy group or the PMR group (13 in the transdiagnostic group and 14 in the PMR group). The experimental group received eight sessions of transdiagnostic therapy while the control group received eight sessions of PMR. The participants completed the following scales: Emotion Regulation Questionnaire, Self-Compassion Scale, Maternal Role Adaptation Scale, and Work and Social Adjustment Scale. Results Transdiagnostic therapy was significantly more effective than PMR in improving emotion regulation strategies, self-compassion, maternal role adaptation, and social and work adjustment based on the between-group comparison conducted at post-test and follow-up ( p < 0.01). Conclusion Transdiagnostic therapy was shown to be effective in improving the emotional health of mothers with premature infants and was more effective than PMR techniques.
Subchondral Bone Plate Classification: A New and More Sensitive Approach for Predicting the Prognosis of Osteonecrosis of the Femoral Head
Authors: Ma Jinhui , Ge Juncheng , Cheng Liming , Wang Bailiang , Yue Debo , Wang Weiguo 
Published: May 2023

Background This study explores the impact of subchondral bone plate necrosis on the development of the osteonecrosis of femoral head (ONFH) and its joint collapse. Methods This retrospective study included 76 ONFH patients (89 consecutive hips) with Association for Research on Osseous Circulation stage II who received conservative treatment without surgical intervention. The mean follow-up time was 15.60 ± 12.29 months. ONFH was divided into 2 types (I and II): Type I with a necrotic lesion involving subchondral bone plate and Type II with a necrotic lesion not involving subchondral bone plate. The radiological evaluations were based on plain x-rays. The data were analyzed using SPSS 26.0 statistical software. Results The collapse rate in Type I ONFH was significantly higher than that in Type II ONFH (P < 0.001). The survival time of hips with Type I ONFH was significantly shorter than those with Type II ONFH and with the endpoint of the femoral head collapse (P < 0.001). The collapse rate of Type I in the new classification (80.95%) was higher compared with that of the China-Japan Friendship Hospital (CJFH) classification (63.64%), and the difference was statistically significant (χ2 = 1.776, P = 0.024). Conclusion Subchondral bone plate necrosis is an important factor that affects ONFH collapse and prognosis. Current classification using subchondral bone plate necrosis is more sensitive for predicting collapse compared with the CJFH classification. Effective treatments should be taken to prevent collapse if ONFH necrotic lesions involve the subchondral bone plate.
Ultrasonographic features of a urinary bladder leiomyoma in a cat
Authors: Biedak Nathan R , Penninck Dominique , Biswell Ethan 
Published: May 2023

Case summary Leiomyoma of the urinary bladder in cats is infrequently documented in the literature, and a description of the ultrasonographic appearance has never been reported. A 9-year-old female spayed cat presented with a 6-month history of intermittent hematuria and progressive pollakiuria. On abdominal ultrasound, a large ovoid mass of mixed echogenicity was seen originating from the ventral apical bladder wall. The mass had faint peripheral hyperechoic radiating striations and was mildly vascularized, especially at its 1.5 cm point of origin from the wall. A partial cystectomy was performed to remove the mass that filled the bladder lumen. Microscopically, the well-demarcated mass extended from the inner muscular layer of the wall into the submucosal layer and was diagnosed as a leiomyoma. Surgical excision was curative. Relevance and novel information This is the first ultrasonographic description of a bladder leiomyoma in a feline patient. Smooth muscle tumors should be included on the differential diagnosis list in future cases with similar ultrasonographic features and chronic lower urinary clinical signs.
Opening the “Black Box”: How Out-of-Class Use of Duolingo Impacts Chinese Junior High School Students’ Intrinsic Motivation for English
Authors: Zeng Cheng , Fisher Linda 
Published: May 2023

Purpose Mobile-assisted language learning (MALL) apps such as Duolingo have great potential for promoting learners’ motivation to learn a second language (L2). However, little research has investigated how this motivational impact takes place. Additionally, despite the flexibility of mobile learning, most existing studies are conducted in classroom settings, with less attention paid to out-of-school technology usage. Design/Approach/Methods To address these gaps, we present a model based on self-determination theory and propose the idea of “motivational transfer” to explain the psychological mechanism underpinning the impact of technology. To examine the model, we conducted a case study with 20 Year 8 Chinese junior school students who used Duolingo to learn English as a foreign language (EFL) after school for 6 weeks. Findings Questionnaire and group interview data support our hypothesized mechanism: learners’ activity-specific intrinsic motivation (IM) for using Duolingo and their underlying psychological need for autonomy and competence can be transferred to a more general level, thereby enhancing learners’ global IM for L2. Originality/Value The proposed theoretical model expands our understanding of how digital technology stimulates learners’ L2 motivation; it can help L2 educators design better technological affordances to promote learners’ motivation both in and outside the classroom.
Coronal alignment in total knee arthroplasty: a review
Authors: Matassi F. , Pettinari F. , Frasconà F. , Innocenti M. , Civinini R. 
Published: May 2023

AbstractTotal knee arthroplasty (TKA) alignment has recently become a hot topic in the orthopedics arthroplasty literature. Coronal plane alignment especially has gained increasing attention since it is considered a cornerstone for improved clinical outcomes. Various alignment techniques have been described, but none proved to be optimal and there is a lack of general consensus on which alignment provides best results. The aim of this narrative review is to describe the different types of coronal alignments in TKA, correctly defining the main principles and terms.
Transcriptome profile changes in the jejunum of nonhuman primates exposed to supralethal dose of total- or partial-body radiation
Authors: Vellichirammal Neetha Nanoth , Sethi Sahil , Avuthu Nagavardhini , Wise Stephen Y. , Carpenter Alana D. , Fatanmi Oluseyi O. , Guda Chittibabu , Singh Vijay K. 
Published: May 2023

AbstractThe risk of exposure of the general public or military personnel to high levels of ionizing radiation from nuclear weapons or radiological accidents is a dire national security matter. The development of advanced molecular biodosimetry methods, those that measure biological response, such as transcriptomics, to screen large populations of radiation-exposed victims is key to improving survival outcomes during radiological mass casualty scenarios. In this study, nonhuman primates were exposed to either 12.0 Gy cobalt-60 gamma (total-body irradiation, TBI) or X-ray (partial-body irradiation, PBI) 24 h after administration of a potential radiation medical countermeasure, gamma-tocotrienol (GT3). Changes in the jejunal transcriptomic profiles in GT3-treated and irradiated animals were compared to healthy controls to assess the extent of radiation damage. No major effect of GT3 on radiation-induced transcriptome at this radiation dose was identified. About 80% of the pathways with a known activation or repression state were commonly observed between both exposures. Several common pathways activated due to irradiation include FAK signaling, CREB signaling in the neurons, phagosome formation, and G-protein coupled signaling pathway. Sex-specific differences associated with excessive mortality among irradiated females were identified in this study, including Estrogen receptor signaling. Differential pathway activation was also identified across PBI and TBI, pointing towards altered molecular response for different degrees of bone marrow sparing and radiation doses. This study provides insight into radiation-induced changes in jejunal transcriptional profiles, supporting the investigation for the identification of biomarkers for radiation injury and countermeasure efficacy.
The role of plasmids in carbapenem resistant E. coli in Alameda County, California
Authors: Walas Nikolina , Slown Samuel , Amato Heather K. , Lloyd Tyler , Bender Monica , Varghese Vici , Pandori Mark , Graham Jay P. 
Published: May 2023

Abstract
Background
Antimicrobial resistant infections continue to be a leading global public health crisis. Mobile genetic elements, such as plasmids, have been shown to play a major role in the dissemination of antimicrobial resistance (AMR) genes. Despite its ongoing threat to human health, surveillance of AMR in the United States is often limited to phenotypic resistance. Genomic analyses are important to better understand the underlying resistance mechanisms, assess risk, and implement appropriate prevention strategies. This study aimed to investigate the extent of plasmid mediated antimicrobial resistance that can be inferred from short read sequences of carbapenem resistant E. coli (CR-Ec) in Alameda County, California. E. coli isolates from healthcare locations in Alameda County were sequenced using an Illumina MiSeq and assembled with Unicycler. Genomes were categorized according to predefined multilocus sequence typing (MLST) and core genome multilocus sequence typing (cgMLST) schemes. Resistance genes were identified and corresponding contigs were predicted to be plasmid-borne or chromosome-borne using two bioinformatic tools (MOB-suite and mlplasmids).

Results
Among 82 of CR-Ec identified between 2017 and 2019, twenty-five sequence types (STs) were detected. ST131 was the most prominent (n = 17) followed closely by ST405 (n = 12). blaCTX−M were the most common ESBL genes and just over half (18/30) of these genes were predicted to be plasmid-borne by both MOB-suite and mlplasmids. Three genetically related groups of E. coli isolates were identified with cgMLST. One of the groups contained an isolate with a chromosome-borne blaCTX−M−15 gene and an isolate with a plasmid-borne blaCTX−M−15 gene.

Conclusions
This study provides insights into the dominant clonal groups driving carbapenem resistant E. coli infections in Alameda County, CA, USA clinical sites and highlights the relevance of whole-genome sequencing in routine local genomic surveillance. The finding of multi-drug resistant plasmids harboring high-risk resistance genes is of concern as it indicates a risk of dissemination to previously susceptible clonal groups, potentially complicating clinical and public health intervention.