bluuebunny/crossref_metadata_2025_split huggingface dataset:
The gut microbiota metabolite capsiate regulate SLC2A1 expression by targeting HIF‐1α to inhibit knee osteoarthritis‐induced ferroptosis
Authors: Guan Zhiyuan , Jin Xiao , Guan Zhiqiang , Liu Shengfu , Tao Kun , Luo Liying 
Published: March 2023

AbstractFerroptosis is an iron‐dependent cell death that has been found to aggravate the progression of osteoarthritis (OA) and gut microbiota‐ OA axis refers to the bidirectional information network between the gut microbiota and OA, which may provide a new way to protect the OA. However, the role of gut microbiota‐derived metabolites in ferroptosis‐relative osteoarthritis remains unclear. The objective of this study was to analyze the protective effect of gut microbiota and its metabolite capsiate (CAT) on ferroptosis‐relative osteoarthritis in vivo and in vitro experiments. From June 2021 to February 2022, 78 patients were evaluated retrospectively and divided into two groups: The health group (n = 39) and the OA group (n = 40). Iron and oxidative stress indicators were determined in peripheral blood samples. And then in vivo and in vitro experiments, a surgically destabilized medial meniscus (DMM) mice model was established and treated with CAT or Ferric Inhibitor‐1 (Fer‐1). Solute Carrier Family 2 Member 1 (SLC2A1) short hairpin RNA (shRNA) was utilized to inhibit SLC2A1 expression. Serum iron was increased significantly but total iron binding capacity was decreased significantly in OA patients than healthy people (p < 0.0001). The least absolute shrinkage and selection operator clinical prediction model suggested that serum iron, total iron binding capacity, transferrin, and superoxide dismutase were all independent predictors of OA (p < 0.001). Bioinformatics results suggested that SLC2A1, Metastasis‐Associated Lung Adenocarcinoma Transcript 1 (MALAT1), and HIF‐1α (Hypoxia Inducible Factor 1 Alpha)‐related oxidative stress signaling pathways play an important role in iron homeostasis and OA. In addition, gut microbiota 16s RNA sequencing and untargeted metabolomics were used to find that gut microbiota metabolites CAT in mice with osteoarthritis were negatively correlated with Osteoarthritis Research Society International (OARSI) scores for chondrogenic degeneration (p = 0.0017). Moreover, CAT reduced ferroptosis‐dependent osteoarthritis in vivo and in vitro. However, the protective effect of CAT against ferroptosis‐dependent osteoarthritis could be eliminated by silencing SLC2A1. SLC2A1 was upregulated but reduced the SLC2A1 and HIF‐1α levels in the DMM group. HIF‐1α, MALAT1, and apoptosis levels were increased after SLC2A1 knockout in chondrocyte cells (p = 0.0017). Finally, downregulation of SLC2A1 expression by Adeno‐associated Virus (AAV) ‐SLC2A1 shRNA improves osteoarthritis in vivo. Our findings indicated that CAT inhibited HIF‐1a expression and reduced ferroptosis‐relative osteoarthritis progression by activating SLC2A1.
Morphometric examination of hind limb and foot bones and fibre type composition of crus region muscles in quail and pigeon
Authors: Isbilir Fatma , Akkoc Cansel Guzin Ozguden , Arıcan Ilker 
Published: March 2023

AbstractIn this study, the foot and hind limb bones of pigeons and quails were measured morphometrically. Additionally, microscopic classifications of the muscles affecting the foot and digit joints were made. For the macroscopic inspection, 40 birds were used, including 20 adult quails (10 males, 10 females) and 20 adult pigeons (10 males, 10 females). Diethyl ether was inhaled to anaesthetize the animals. The poultry animals were put under anaesthesia, and radiographic pictures of their left feet were obtained individually. DAP measurements were performed separately from the images taken with the Image J program. Then, they were euthanized by cervical dislocation under diethyl ether anaesthesia. The right legs of the euthanized animals were preserved in a 10% neutral formalin solution for histology procedures just after the legs were dissected from the trunk. Morphometric measurements of bone lengths were made in accordance with the measurement points specified by von den Driesch. After fixation for histological examination, routine tissue follow‐up was performed and the tissues were embedded in paraffin. The presence of SO‐type I, FG‐type IIb and FOG‐type IIa in 4–5 μ sections taken from paraffin blocks was demonstrated using the indirect streptavidin‐biotin‐complex method from immunohistochemical methods. The result of our study was statistically evaluated at p < 0.05 and p < 0.001 levels. The length of the hallux, the articulation point to the TMT and the fibre arrangements in the two flexor group muscles showed that the hind limbs and feet of the pigeons had a more favourable anatomical and histological structure for the perching movement.
An annotated ventricular tachycardia (VT) alarm database: Toward a uniform standard for optimizing automated VT identification in hospitalized patients
Authors: Pelter Michele M. , Carey Mary G. , Al‐Zaiti Salah , Zegre‐Hemsey Jessica , Sommargren Claire , Isola Lamberto , Prasad Priya , Mortara David , Badilini Fabio 
Published: March 2023

AbstractBackgroundFalse ventricular tachycardia (VT) alarms are common during in‐hospital electrocardiographic (ECG) monitoring. Prior research shows that the majority of false VT can be attributed to algorithm deficiencies.PurposeThe purpose of this study was: (1) to describe the creation of a VT database annotated by ECG experts and (2) to determine true vs. false VT using a new VT algorithm created by our group.MethodsThe VT algorithm was processed in 5320 consecutive ICU patients with 572,574 h of ECG and physiologic monitoring. A search algorithm identified potential VT, defined as: heart rate >100 beats/min, QRSs > 120 ms, and change in QRS morphology in >6 consecutive beats compared to the preceding native rhythm. Seven ECG channels, SpO2, and arterial blood pressure waveforms were processed and loaded into a web‐based annotation software program. Five PhD‐prepared nurse scientists performed the annotations.ResultsOf the 5320 ICU patients, 858 (16.13%) had 22,325 VTs. After three levels of iterative annotations, a total of 11,970 (53.62%) were adjudicated as true, 6485 (29.05%) as false, and 3870 (17.33%) were unresolved. The unresolved VTs were concentrated in 17 patients (1.98%). Of the 3870 unresolved VTs, 85.7% (n = 3281) were confounded by ventricular paced rhythm, 10.8% (n = 414) by underlying BBB, and 3.5% (n = 133) had a combination of both.ConclusionsThe database described here represents the single largest human‐annotated database to date. The database includes consecutive ICU patients, with true, false, and challenging VTs (unresolved) and could serve as a gold standard database to develop and test new VT algorithms.
The Romantic Anti‐Capitalisms of Short‐Term Rental Hosting
Authors: Medvedeva Nina 
Published: March 2023

AbstractThis article focuses on how Airbnb hosts in the Boston metro, San Francisco, and Washington, DC respond to legislative short‐term rental (STR) regulations. I trace how hosts justify their multiple STR practices by way of the civic good all while foreclosing a debate about the potential negative effects of their monetisation. I show how hosts displace the displeasing aspects of STRs onto the abstracted category of the “Investor Host”, a stand‐in for foreign investment capital and indifferent corporations. I argue that STR owners replicate what Iyko Day calls “romantic anti‐capitalism”, a mode of critiquing capitalism that valorises the local and concrete uses of capital while displacing capital's destructive effects onto an abstracted and racialised other. I build on Day's theorisation by demonstrating how there are multiple genres of romantic anti‐capitalism that function differently based on a person's structural position within settler‐colonialism.
Use of a gene expression signature to identify trimetazidine for repurposing to treat bipolar depression
Authors: Bortolasci Chiara C. , Kidnapillai Srisaiyini , Spolding Briana , Truong Trang T. T. , Connor Timothy , Swinton Courtney , Panizzutti Bruna , Liu Zoe S. J. , Sanigorski Andrew , Dean Olivia M. , Crowley Tamsyn , Richardson Mark , Bozaoglu Kiymet , Vlahos Katerina , Cowdery Stephanie , Watmuff Brad , Steyn Stephan F. , Wolmarans De Wet , Engelbrecht Barend J. , Perry Christina , Drummond Katherine , Pang Terence , Jamain Stéphane , Gray Laura , McGee Sean L. , Harvey Brian H. , Kim Jee Hyun , Leboyer Marion , Berk Michael , Walder Ken 
Published: March 2023

AbstractObjectivesThe aim of this study was to repurpose a drug for the treatment of bipolar depression.MethodsA gene expression signature representing the overall transcriptomic effects of a cocktail of drugs widely prescribed to treat bipolar disorder was generated using human neuronal‐like (NT2‐N) cells. A compound library of 960 approved, off‐patent drugs were then screened to identify those drugs that affect transcription most similar to the effects of the bipolar depression drug cocktail. For mechanistic studies, peripheral blood mononuclear cells were obtained from a healthy subject and reprogrammed into induced pluripotent stem cells, which were then differentiated into co‐cultured neurons and astrocytes. Efficacy studies were conducted in two animal models of depressive‐like behaviours (Flinders Sensitive Line rats and social isolation with chronic restraint stress rats).ResultsThe screen identified trimetazidine as a potential drug for repurposing. Trimetazidine alters metabolic processes to increase ATP production, which is thought to be deficient in bipolar depression. We showed that trimetazidine increased mitochondrial respiration in cultured human neuronal‐like cells. Transcriptomic analysis in induced pluripotent stem cell‐derived neuron/astrocyte co‐cultures suggested additional mechanisms of action via the focal adhesion and MAPK signalling pathways. In two different rodent models of depressive‐like behaviours, trimetazidine exhibited antidepressant‐like activity with reduced anhedonia and reduced immobility in the forced swim test.ConclusionCollectively our data support the repurposing of trimetazidine for the treatment of bipolar depression.
Emotion transmission in peer dyads in middle childhood
Authors: Hubbard Julie A. , Moore Christina C. , Zajac Lindsay , Bookhout Megan K. , Dozier Mary 
Published: March 2023

AbstractThis study investigated emotion transmission among peers during middle childhood. Participants included 202 children (111 males; race: 58% African American, 20% European American, 16% Mixed race, 1% Asian American, and 5% Other; ethnicity: 23% Latino(a) and 77% Not Latino(a); Mincome = $42,183, SDincome = $43,889; Mage = 9.49; English‐speaking; from urban and suburban areas of a mid‐Atlantic state in the United States). Groups of four same‐sex children interacted in round‐robin dyads in 5‐min tasks during 2015–2017. Emotions (happy, sad, angry, anxious, and neutral) were coded and represented as percentages of 30‐s intervals. Analyses assessed whether children's emotion expression in one interval predicted change in partners' emotion expression in the next interval. Findings suggested: (a) escalation of positive and negative emotion [children's positive (negative) emotion predicts an increase in partners' positive (negative) emotion], and (b) de‐escalation of positive and negative emotion (children's neutral emotion predicts a decrease in partners' positive or negative emotion). Importantly, de‐escalation involved children's display of neutral emotion and not oppositely valenced emotion.
The TGFβ system and TIMP1 and 3 genotypes in Turner syndrome—Relation with aortic congenital malformations
Authors: Ridder Lukas Ochsner , Stochholm Kirstine , Mortensen Kristian Havmand , Andersen Niels Holmark , Gravholt Claus Højbjerg 
Published: March 2023

AbstractObjectiveCardiovascular complications and congenital malformations are known traits in Turner syndrome (TS), which increases mortality. Women with TS have varying phenotype and cardiovascular risks. A biomarker assessing the risk for cardiovascular complications could potentially reduce mortality in high‐risk TS and reduce screening in TS participants with low cardiovascular risk.Design, Patients, Participants and MeasurementsAs part of a study initiated in 2002, 87 TS participants and 64 controls were invited to magnetic resonance imaging of the aorta, anthropometry, and biochemical markers. TS participants were re‐examined thrice lastly in 2016. The focus of this paper is the additional measurements of transforming growth factor beta (TGFβ), matrix metalloproteinase (MMP's), tissue inhibitor of matrix metalloproteinase (TIMP), peripheral blood DNA and their associations with TS and the cardiovascular risk and congenital heart disease.ResultsTS participants had lower TGFβ1 and TGFβ2 values compared to controls. snp11547635 heterozygosity was not associated with any biomarkers but was associated with increased risk of aortic regurgitation. TIMP4 and TGFβ1 were correlated with the aortic diameter at several measuring positions. During follow‐up, the antihypertensive treatment decreased the descending aortic diameter and increased TGFβ1 and TGFβ2 levels in TS.ConclusionTGFβ and TIMP's are altered in TS and may play a role in the development of coarctation and dilated aorta. snp11547635 heterozygosity was not found to impact biochemical markers. Further studies should investigate these biomarkers to further unravel the pathogenesis of the increased cardiovascular risk in TS participants.
Age and sex differentially shape brain networks in Parkinson's disease
Authors: Chen Zhichun , Wu Bin , Li Guanglu , Zhou Liche , Zhang Lina , Liu Jun 
Published: March 2023

AbstractAimsAge and sex are important individual factors modifying the clinical symptoms of patients with Parkinson's disease (PD). Our goal is to evaluate the effects of age and sex on brain networks and clinical manifestations of PD patients.MethodsParkinson's disease participants (n = 198) receiving functional magnetic resonance imaging from Parkinson's Progression Markers Initiative database were investigated. Participants were classified into lower quartile group (age rank: 0%~25%), interquartile group (age rank: 26%~75%), and upper quartile group (age rank: 76%~100%) according to their age quartiles to examine how age shapes brain network topology. The differences of brain network topological properties between male and female participants were also investigated.ResultsParkinson's disease patients in the upper quartile age group exhibited disrupted network topology of white matter networks and impaired integrity of white matter fibers compared to lower quartile age group. In contrast, sex preferentially shaped the small‐world topology of gray matter covariance network. Differential network metrics mediated the effects of age and sex on cognitive function of PD patients.ConclusionAge and sex have diverse effects on brain structural networks and cognitive function of PD patients, highlighting their roles in the clinical management of PD.
Living donor liver transplantation: A multi‐disciplinary collaboration towards growth, consensus, and a change in culture
Authors: Liapakis AnnMarie , Jesse Michelle T , Pillai Anjana , Bittermann Therese , Dew Mary Amanda , Emre Sukru , Hunt Heather , Kumar Vineeta , Locke Jayme , Mohammad Saeed , Olthoff Kim , Verna Elizabeth C. , Lentine Krista L ,   LDLT Consensus Conference Working Group
Published: March 2023

AbstractIntroductionLiving donor liver transplantation (LDLT) reduces liver transplant waitlist mortality and provides excellent long‐term outcomes for persons with end stage liver disease. Yet, utilization of LDLT has been limited in the United States (US).MethodsIn October 2021, the American Society of Transplantation held a consensus conference to identify important barriers to broader expansion of LDLT in the US, including data gaps, and make recommendations for impactful and feasible mitigation strategies to overcome these barriers. Domains addressed encompassed the entirety of the LDLT process. Representation from international centers and living donor kidney transplantation were included for their perspective/experience in addition to members across disciplines within the US liver transplantation community. A modified Delphi approach was employed as the consensus methodology.ResultsThe predominant theme permeating discussion and polling results centered on culture; the beliefs and behaviors of a group of people perpetuated over time.ConclusionsCreating a culture of support for LDLT in the US is key for expansion and includes engagement and education of stakeholders across the spectrum of the process of LDLT. A shift from awareness of LDLT to acknowledgement of benefit of LDLT is the primary goal. Propagation of the maxim "LDLT is the best option" is pivotal.
Alteration of neural activity and neuroinflammatory factors in the insular cortex of mice with corneal neuropathic pain
Authors: Xu Rui , Zhang Yu‐Wen , Gu Qing , Yuan Tian‐Jie , Fan Bing‐Qian , Xia Jun‐Ming , Wu Jin‐Hong , Xia Ying , Li Wen‐Xian , Han Yuan 
Published: March 2023

AbstractDry eye disease (DED) affects nearly 55% of people worldwide; several studies have proposed that central sensitization and neuroinflammation may contribute to the developing corneal neuropathic pain of DED, while the underlying mechanisms of this contribution remain to be investigated. Excision of extra orbital lacrimal glands established the dry eye model. Corneal hypersensitivity was examined through chemical and mechanical stimulation, and open field test measured the anxiety levels. Restingstate fMRI is a method of functional magnetic resonance imaging (rs‐fMRI) was performed for anatomical involvement of the brain regions. The amplitude of low‐frequency fluctuation (ALFF) determined brain activity. Immunofluorescence testing and Quantitative real‐time polymerase chain reaction were also performed to further validate the findings. Compared with the Sham group, ALFF signals in the supplemental somatosensory area, secondary auditory cortex, agranular insular cortex, temporal association areas, and ectorhinal cortex brain areas were increased in the dry eye group. This change of ALFF in the insular cortex was linked with the increment in corneal hypersensitivity (p < 0.01), c‐Fos (p < 0.001), brain‐derived neurotrophic factor (p < 0.01), TNF‐α, IL‐6, and IL‐1β (p < 0.05). In contrast, IL‐10 levels (p < 0.05) decreased in the dry eye group. DED‐induced corneal hypersensitivity and upregulation of inflammatory cytokines could be blocked by insular cortex injection of Tyrosine Kinase receptor B agonist cyclotraxin‐B (p < 0.01) without affecting anxiety levels. Our study reveals that the functional activity of the brain associated with corneal neuropathic pain and neuroinflammation in the insular cortex might contribute to dry eye‐related corneal neuropathic pain.
Stocking method effects on forage accumulation, canopy attributes, and nutritive value of grazed Mulato II brachiariagrass pastures
Authors: de Almeida Otávio Goulart , Pedreira Carlos Guilherme Silveira , Holschuch Solange Garcia , Pedroso Gabriel Baracat , Yasuoka Junior Issamu , da Silva Valdson José 
Published: March 2023

AbstractRotational stocking (RS) is generally associated with the intensification of pasture‐based animal production systems, although many studies have shown little advantage over continuous stocking (CS). The objective of this research was to describe and explain the effects of two average canopy heights (20 and 30 cm) and three stocking methods (CS; rotational stocking with lenient defoliation, RSL; and rotational stocking with moderate defoliation, RSM) on forage accumulation (FA), vertical distribution of plant‐part components, and nutritive value of ‘Mulato II’ hybrid brachiariagrass (Brachiaria spp. syn. Urochloa spp.) during two summer rainy seasons in Piracicaba, São Paulo, Brazil. Pastures were maintained at average canopy height of 20 and 30 cm under CS, and treatments under RSL and RSM were imposed by variations of ±20 and 30% of the average canopy heights, representing defoliation intensities of 33 and 47% of the pre‐graze heights, respectively. The FA was not affected by CS, RSL, RSM stocking methods, averaging 8090 kg DM ha−1 year−1. Canopy bulk density and distribution of plant‐part components in the canopy profile were better at the average canopy height of 20 cm, accompanied by the greater nutritive value. The leaf bulk density was generally greater in RSM compared to CS. Pastures under CS had greater crude protein and in vitro digestible matter, and lesser neutral detergent fibre concentrations. Mulato II brachiariagrass should be managed at an average height of 20 cm under CS.
Microbiome and function alterations in the gastric mucosa of asymptomatic patients with Helicobacter pylori infection
Authors: Yu Tingting , Lu Tianyu , Deng Wei , Yao Danping , He Cheng , Luo Peng , Song Jian 
Published: March 2023

AbstractBackgroundMost patients with Helicobacter pylori (H. pylori) infection have no clinical symptoms, numerous studies reported the gastric microbiome in H. pylori‐infected patients, but asymptomatic patients have not been distinguished. How the microbiome and function changes in asymptomatic patients with H. pylori infection remains poorly understood.MethodsA total of 29 patients were divided into H. pylori‐infected asymptomatic group (10 patients), H. pylori‐infected symptomatic group (11 patients) and H. pylori‐uninfected group (8 patients). Gastric mucosa specimens were taken for histopathological examination, special staining, and 16 S rDNA sequencing. High‐throughput results were evaluated by community composition analysis, indicator species analysis, alpha diversity analysis, beta diversity analysis, and function prediction.ResultsThe gastric microbiota composition at phylum and genus level of H. pylori‐infected asymptomatic patients were similar with H. pylori‐infected symptomatic group, but different from H. pylori‐uninfected patients. The diversity and richness of gastric microbial community declined significantly in H. pylori‐infected asymptomatic group comparing with H. pylori‐uninfected group. Sphingomonas may be an indicator between symptomatic and asymptomatic patients with H. pylori infection, the AUC value of Sphingomonas is 0.79. Interactions between species increased and altered notably after H. pylori infection. More genera were affected by Helicobacter in H. pylori‐infected asymptomatic patients. The function condition changed significantly in asymptomatic patients with H. pylori infection, there was no difference comparing with symptomatic ones. Amino acid metabolism and lipid metabolism strengthened but carbohydrate metabolism remained constant after H. pylori infection. The metabolism of fatty acid and bile acid was disturbed after infection with H. pylori.ConclusionThe gastric microbiota composition and function mode changed significantly after H. pylori infection regardless of the presence of clinical symptoms, there was no difference between H. pylori‐infected asymptomatic and symptomatic patients. The difference in gastric microbiota composition and interactions between species might be responsible for presence of digestive symptoms.