bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skimmianine attenuates liver ischemia/reperfusion injury by regulating PI3K-AKT signaling pathway-mediated inflammation, apoptosis and oxidative stress
Authors: Huo Chenglong , Wang Bing , Zhang Xuewen , Sun ZhenGang 
Published: March 2023

Abstract
Liver ischemia/reperfusion (I/R) injury is a common injury after liver transplantation and hepatectomy. Skimmianine (Ski) has antibacterial, antiviral pharmacological effects. However, it is not clear whether Ski has a protective effect against liver I/R injury. In the present study, we established mouse liver I/R model and AML12 cell hypoxia-reoxygenation (H/R) model pretreated with different concentrations of Ski. Serum transaminase levels, necrotic liver area, cell viability, inflammatory factors, oxidative stress and apoptosis-related levels were measured to assess the protective effect of Ski against liver I/R injury. Western blotting was used to detect apoptosis-related proteins and PI3K-AKT pathway-related proteins. Mice and cells were also treated with PI3K inhibitor LY294002 to assess changes in indicators of liver injury. The results showed that Ski significantly reduced transaminase levels, liver necrosis area, oxidative stress and apoptosis levels in mice with I/R. Ski also inhibited cell injury and apoptosis after H/R. Ski also activated phosphorylation of PI3K-AKT pathway-related proteins after liver I/R and cell H/R. PI3K inhibitor LY294002 could well reverse the alleviation of I/R injury caused by Ski. These results confirm that Ski exerts a protective effect against liver I/R injury through activation of the PI3K-AKT pathway.
A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
Authors: Wranik Maximilian , Kepa Michal , Beale Emma , James Daniel , Bertrand Quentin , Weinert Tobias , Furrer Antonia , Glover Hannah , Gashi Dardan , Carrillo Melissa , Kondo Yasushi , Stipp Robin , Khusainov Georgii , Nass Karol , Ozerov Dmitry , Cirelli Claudio , Johnson Philip , Dworkowski Florian , Beale John , Stubbs Scott , Zamofing Thierry , Schneider Marco , Krauskopf Kristina , Gao Li , Thorn-Seshold Oliver , Bostedt Christoph , Bacellar Camila , Steinmetz Michel , Milne Christopher , Standfuss Jörg 
Published: March 2023

Abstract
Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such experiments. Here, we describe a multi-reservoir, high viscosity extruder as a step towards automation of sample delivery at XFELs. Compared to a standard single extruder, sample exchange time was halved and the workload of users was greatly reduced. In-built temperature control of samples facilitated optimal extrusion and offered a path forward for the observation of temperature-dependent structural changes. After commissioning the device with lysozyme crystals, we collected time-resolved data using crystals of a membrane-bound, light-driven sodium pump. Static data were also collected from the soluble protein tubulin that was soaked with a series of small molecule drugs. Using these data, we identified low occupancy (as little as 30%) ligands using a minimal amount of data from a serial crystallography experiment, a result that could be exploited for structure-based drug design.
Texture evolution and tensile anisotropy of TC17 alloy β forging pancake
Authors: Deng Yuting , Li Siqing , Wang Xu 
Published: March 2023

Abstract
The crystallographic orientations of grain boundary α phase and matrix β phase are two main factors determining the mechanical properties of β forging TC17 alloy. Microstructure and texture evolution of three height reduction pancakes were mainly investigated in this present work by optical microscopy (OM), scanning electron microscopy (SEM), electron back-scattered diffraction (EBSD) combined with Schmid factor analysis and X-ray diffraction (XRD). The results revealed that all pancakes of three height reduction exhibited similar < 001>//forging direction(FD) fiber texture component of β matrix phase whose intensity significantly increased with the increase of height reduction from 20–50%. While the height reduction was up to 70%, intensity basically remained unchanged, but a new texture component (< 011>//FD) was formed possibly due to dynamic recrystallization. The recrystallized grains representing the undeformed or slightly deformed grains affect the texture component of β matrix. Tensile properties of β forging TC17 alloy demonstrated relatively obvious anisotropy. The angle of 45° between forging direction and longitudinal direction indicated the highest strength and lowest ductility, while the longitudinal direction showed higher strength than forging direction. The orientation diversity of grain boundary α phase increased with the increase of height reduction. The specific crystallographic features of grain boundary α phase caused by forging texture affect effective slip length. In addition, their Schmid factor varied significantly with loading direction, possibly leading to tensile anisotropy.
Analysis of Widefield Choroidal Thickness Maps of Healthy Eyes using Swept Source Optical Coherence Tomography
Authors: Hirano Masatoshi , Muraoka Yuki , Kogo Takahiro , Ishikura Masaharu , Nishigori Naomi , Ueda-Arakawa Naoko , Miyata Manabu , Hata Masayuki , Takahashi Ayako , Miyake Masahiro , Tsujikawa Akitaka 
Published: March 2023

Abstract
We aimed to obtain widefield (WF) swept source optical coherence tomography (SS-OCT) data and examine the features of choroidal thickness maps of healthy eyes. The posterior pole choroidal thickness was examined for 127 eyes using enhanced-depth imaging (EDI) of SS-OCT with a viewing angle of 20 (vertical) × 23 (horizontal) mm, and choroidal thickness maps were generated. For SS-OCT image analysis, we developed a grid with inner and outer rings, each divided into superotemporal, inferotemporal, superonasal, and inferonasal quadrants, respectively, making up a total of nine subfields including the central 3-mm ring. The posterior pole choroidal thicknesses were significantly lesser at the periphery than in the central area, in the inferior field than in the superior field, and in the nasal field than in the temporal field (p < 0.001 for all). We also evaluated the effects of age and axial length (AL) on the WF choroidal thickness. Choroidal thicknesses in all subfields were negatively associated with advanced age (p < 0.05). Choroidal thicknesses in the central subfield and the inferonasal inner and outer subfields were negatively associated with AL (p = 0.042, 0.034, and 0.022, respectively). These findings provide insights into the two-dimensional characteristics of the choroidal thickness and their associations with age and AL.
A traffic light control method based on multi-agent deep reinforcement learning algorithm
Authors: Liu Dongjiang , Li Leixiao 
Published: March 2023

Abstract
An efficient way to cope with traffic congestion is Intelligent Traffic Light Control (ITLC). Particularly, multi-agent based intelligent traffic light control algorithms are more popular and efficient than others. But there are still some problems in these algorithms. Firstly, an efficient communication mechanism is needed. As current traffic condition of an intersection can impact other intersections' future traffic condition, every agent should know the traffic condition of other intersections through communication. Thus, a new communication mechanism should be designed. By using this mechanism, traffic condition of an intersection can be passed to several relevant agents easily. And traffic condition of an intersection can be described simply and clearly. Secondly, asynchronization of message should be considered while processing received messages. As duration time of traffic lights' cycles is different from agent to agent and message sending event happens at the end of each traffic light cycle, different agents will send message at different time. Moreover, every agent tries to process the received messages at the end of each traffic light cycle as well. So it is hard for an agent to decide which message is the latest one and the most valuable. Thirdly, a new reward calculating method should be designed. In the traditional reinforcement learning based ITLC algorithms, only queue length of congested cars or waiting time of these cars is considered while calculating reward value. But, both of them are very important for measuring the level of traffic congestion. So a new reward calculation method is needed. To solve all these problems, in this paper, a new ITLC algorithm is proposed. At the same time, a new message sending and processing method is proposed and adopted by this ITLC algorithm. The first and the second problems described above are tried to be solved by using this new method. Besides, to measure traffic congestion in a more reasonable way, a new reward calculation method is proposed and used. This method takes both waiting time and queue length into consideration.
In silico discovery of potential sodium–glucose cotransporter-2 inhibitors from natural products for Prevent Kidney Failure in Patients with Type 2 Diabetes
Authors: Shakour Neda , Hoseinpoor Saeideh , Iranshahi Mehrdad , Badpeyma Mohaddeseh , Hadizadeh Farzin 
Published: March 2023

Abstract

Associated with Type 2 diabetes (T2DM), renal dysfunction contributes to an increased death rate. Consequently, it would appear that preventing the advancement of renal disease is crucial in the treatment of diabetic patients. SGLT2 inhibitors have been linked to reduced renal mortality, decreased hospitalization, and slowed the progression of renal impairment and albuminuria. The objective of this study was aimed to identify natural SGLT2 inhibitors using an in silico evaluation of the compounds of zinc database using structure-based virtual screening. Using pharmacophore modelling of the standard drug, a total of 1,1336 natural compounds that have the potential to act as SGLT2 inhibitors were identified; six of these compounds,580,1131,212,357,822, and306, had a similar docking affinity to the four known SGLT2 inhibitors. The top two finds,580and306, were chosen due to the convenience of the pharmacokinetic characteristics from the absorption, distribution, metabolism and excretion (ADME), oral bioavailability, and parameters from molecular dynamics simulation (MD). Compound580was discovered as a potential treatment candidate after estimations of the metabolic processes and cardiotoxicity. This study may assist in the advancement of both in vitro and in vivo validation, as well as the development of new SGLT2 inhibitors.
Applications of Boron cluster Supramolecular Frameworks as Metal-free Chemodynamic Therapy Agents
Authors: Xu Xiaoran , Deng Xuefan , Li Yi , Xia Shiying , Baryshnikov Glib , Bondarchuk Sergey , Ågren Hans , Wang Xin Yu , Liu Pan , Tan Yujia , Huang Tianhe , Zhang Haibo , Wei Yongchang 
Published: March 2023

Abstract
Chemodynamic therapy (CDT) is a highly targeted approach to treat cancer since it converts hydrogen peroxide into harmful hydroxyl radicals (•OH) through Fenton or Fenton-like reactions in tumor microenvironments. However, the systemic toxicity of metal-based CDT agents has limited their clinical use due to harmful side effects and low efficiency. Herein, we generate a novel CDT agent: 2,4,6-tri(4-pyridyl)-1,3,5-triazine (TPT)/ [closo-B12H12]2−(TPT@ B12H12), which is metal-free and may avoid cumulative toxicity during long-term therapy. We investigated the Fenton-like catalytic activity and anti-melanoma effects of the synthesized boron cluster supramolecular framework TPT@B12H12, and the results showed that it could effectively suppress the melanoma growth both in vitro and in vivo through ROS generation. The favorable properties of the TPT@B12H12 system were rationalized by means of quantum chemistry DFT calculations revealing that TPT@B12H12 substantially decreases the activation barrier compared to the effective Fe2+ ion commonly used in Fenton reactions. This study highlights the great clinical translational potential of TPT@B12H12 as a CDT agent, potentially serving as a prelude to the rapid development of metal-free CDT agents.
Directional excitation of acoustic graphene plasmons using oblique incidences
Authors: SUN BIN , XIE FEIFENG , ZHOU WEILU , TU FEIQUAN , TAN ZHIYUN , KANG SHUAI , FANG ZHENXING , LIU JIANQIANG 
Published: March 2023

Abstract
In this work, the directional excitation of acoustic graphene plasmons (AGPs) are numerically studied using Finite Element Methods. In our proposed hybrid graphene-metal structure under oblique incidences, not only are AGPs excited efficiently, but also they are unidirectional propagating along a graphene monolayer. Although the symmetry AGPs dispersion relations are broken by oblique incidence, both left and right moved AGPs are excited simultaneously at a resonant wavelength due to almost equaled wavenumbers of directional propagated AGPs. Based on the fact that great AGPs excitation efficiency can’t guarantee high EM energy propagating in one direction, we will focus on how the directional propagating net energy are affected by geometrical parameters. Due to the tunable graphene conductivity, AGPs propagation with great unidirectional net energy can be dynamically controlled by a relatively low externally applied bias voltage (electrostatic gating). The prototype structure may find applications in ultra-confined plasmons launchers and switchers in integrated optics.
Carcinosarcoma of Parotid Gland: An extremely rare malignancy with an unusual carcinomatous component- A case report with review of literature
Authors: Jain Swachi , Abdelwahed Mohammed , Chavarria Daniel Hector , Pereira Lucio , Stone Gary , Johnson Alan , Li Jian Yi 
Published: March 2023

Abstract
Background
Carcinosarcoma of the parotid gland is an extremely rare malignancy comprising of 0.04-0.16% of all salivary gland tumors. They consist of distinct carcinomatous and sarcomatous components and may arise de novo or from a preexisting pleomorphic adenoma.
Methods and results
Herein we present a case of an 80-year-old female who presented with progressively increasing left facial swelling over 6 weeks. Magnetic Resonance Imagining revealed a mass (3.4 cm) in the parotid gland with a predominant cystic/necrotic component. The cytology was atypical (Milan3) and a total parotidectomy and selective lymph node dissection was done. The resection showed extensive necrosis with high grade sarcomatous (chondrosarcoma) areas. The epithelial component was adenoid cystic carcinoma with perineural invasion. The patient is currently undergoing radiotherapy of the tumor bed and skull base due to propensity of perineural invasion of the adenoid cystic component. The most common carcinomas in carcinosarcomas of salivary glands are adenocarcinoma and squamous cell carcinoma.
Conclusion
This is the first case of an adenoid cystic carcinoma with chondrosarcoma to the best of our knowledge. Carcinosarcoma is a high-grade aggressive lesion with a poor prognosis and should be treated aggressively. More studies are needed to understand the origin of these tumors.
Morphologic, phenotypic, and genotypic similarities between primary tumors and corresponding 3D cultures grown in a repeatable system
Authors: Muscatello Luisa Vera , Frabetti Stella , Avallone Giancarlo , Gobbo Francesca , Pasquini Arianna , D'Annunzio Giulia , Pisoni Luciano , Marconato Laura , Terragni Rossella , Biase Dario , Candini Olivia , Sarli Giuseppe 
Published: March 2023

Abstract

Background:Three-dimensional (3D) cell cultures are the new frontier for reproducing the tumor micro-environmentin vitro. The aims of the study were (1) to establish primary 3D cell cultures from canine spontaneous neoplasms and (2) to demonstrate the morphological, phenotypic and genetypic similarities between the primary canine neoplasms and the corresponding 3D cultures, through the expression of tumor differentiation markers.Results:Seven primary tumors were collected, including 4 carcinomas and 3 soft tissue sarcomas. 3D cell cultures reproduced the morphological features of the primary tumors and showed an overlapping immunophenotype of the primary epithelial tumors. Immunohistochemistry demonstrated the growth of stromal cells and macrophages admixed with the neoplastic epithelial component, reproducing the tumor microenvironment. Mesenchymal 3D cultures reproduced the immunophenotype of the primary tumor completely in 2 out of 3 examined cases while a discordant expression was documented for a single marker in one case. No single nucleotide variants or small indel were detected in TP53 or MDM2 genes, both in primary tumors and in 3D cell cultures specimens. In one sample, MDM2 amplicons were preferentially amplified compared to TP53 ones, indicating amplification of MDM2, detectable both in the primary tumor and in the corresponding cell culture specimen.Conclusion: Here we demonstrate a good cell morphology, phenotype and genetic profile overlap between primary tumors and the corresponding 3D cultures grown in a repeatable system.
A key component of the early divisome, SepF, integrates spatial cues and directly links polar growth and cell division in Streptomyces
Authors: Cassettari Gemma , Tan Xiao , Lau Pak , Moye Daniel , Harper Stefan , Lézé Bertrand , Burrows Lucy , Alcock Emily , Bywater-Brenna Kavana , Bone Benjamin , Jonylaite Dovile , Kelemen Gabriella 
Published: March 2023

Abstract
The complex life-cycle of Streptomyces bacteria begins with filamentous growth governed by a polar assembly of proteins, including Scy. Following growth phase, the key division protein FtsZ initiates synchronous septation that transforms the multi-nucleoidal hyphae into uni-nucleoidal pre-spore compartments. The assembly of regularly-spaced FtsZ rings during sporulation is a fascinating example of bacterial cellular organisation, yet to be fully understood.
Here we establish that in this filamentous model bacterium the formation of the cytokinetic ring requires not only FtsZ but also SepF. Moreover, despite the temporal and spatial separation of cell division and polar growth during the cell cycle, we demonstrate a direct interaction between SepF and Scy, both in vivo and in vitro. We propose that the dynamic interplay between Scy and SepF is not only critical for the nucleation of the cytokinetic ring but also for positioning the polarity centres for the emergence of future germ tubes.
Mother’s Late Return Home from Work, Family Relationship, and Non-cognitive Skills of Children: Evidence from Japanese Adolescents
Authors: Seki Mai , Shoji Masahiro , Yamasaki Izumi 
Published: March 2023

Abstract
Although previous studies examine the association between maternal employment status and child development, their findings are mixed and the mechanisms are not well understood. A potential explanation for the mixed results could be the omission of the time of returning home from work among working mothers, but this conjecture has not been tested in the literature. Using a nationwide child–parent survey in Japan (N=4,757), this study examines the association between mothers’ time of returning home and their children’s locus of control. The results of the entropy balancing method demonstrate that the daughters of mothers who return after 7 pm are more likely to believe that they cannot control their life outcomes, while such an association is not observed for mothers who return by 7 pm. This relationship is mediated by the deterioration of family relationships. In line with theoretical predictions, the negative association is more severe among households with high socioeconomic status, while it is mitigated if fathers return home early or children co-habit with their grandparents. These findings are relevant for policymakers, given the increasing number of married women in full-time and managerial positions and the diffusion of teleworking.
JEL Classification: D91, J13, J22
Stromal cells associated with soft tissue augmentation by a volume stable collagen matrix (VCMX) are predominated by anti-inflammatory/reparative macrophages
Authors: Artzi Zvi , Vered Marilena , Maymon-Gil Tal , Renert Uri , Netanely Erez , Thoma Daniel S. 
Published: March 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Two-step Inertial Method for Solving Split Common Null Point Problem With Multiple Output Sets in Hilbert Spaces
Authors: OKEKE C. C. , ADAMU A 
Published: March 2023

Abstract
In this paper, two-step inertial extrapolation and self-adaptive step sizes is proposed to solve the split common null point problem with multiple output sets in Hilbert spaces. Weak convergence analysis are obtained under some easy to verify conditions on the iterative parameters in Hilbert spaces. Preliminary numerical tests are performed to support the theoretical analysis of our proposed method.
The Accuracy of Intravascular Ultrasound-Derived Virtual Fractional Flow Reserve and FFR Derived from Computed Tomography for Functional Assessment of Coronary Artery Disease
Authors: Huang Wenhao , Zhang Jingyuan , Yang Lin , Hu Yumeng , Leng Xiaochang , Liu Yajun , Jin Hongfeng , Tang Yiming , Wang Jiangting , Liu Xiaowei , Guo Yitao , Ye Chen , Feng Yue , Xiang Jianping , Tang Lijiang , Du Changqing 
Published: March 2023

Abstract
Background: Coronary computed tomography-derived fractional reserve fraction (CT-FFR) and intravascular ultrasound-derived fractional reserve fraction (IVUS-FFR) are methods of functional assessment based on coronary imaging; however, the calculation algorithms are substantially different between them. The aim of this study was to compare diagnostic performance between CT-FFR and IVUS-FFR using invasive fractional flow reserve (FFR) as the reference standard.
Method: Our retrospective study enrolled 40 patients (41 lesions) with known or suspected coronary artery disease (CAD) between January 2020 and July 2021. Patients who completed coronary CT angiography (CCTA) within six months underwent intravascular ultrasound (IVUS) and fractional flow reserve (FFR) evaluation. We used a novel CFD-based model (AccuFFRct, ArteryFlow Technology Co., Ltd., Hangzhou, China) to compute the CT-FFR values, and a novel software (AccuFFRivus, ArteryFlow Technology Co., Ltd., Hangzhou, China) to calculate the IVUS-FFR values coupled with angiography. The diagnostic performance of CT-FFR and IVUS-FFR was evaluated using invasive FFR≤0.8 as the reference standard.
Result: AccuFFRivus demonstrated a strong correlation with invasive FFR (R=0.7913, P<0.0001), and AccuFFRct was also highly associated with invasive FFR (R=0.6296, P<0.0001). Both AccuFFRivus and AccuFFRct showed good agreement with FFR. The area under the ROC curve was 0.960 (95%CI:0.833-0.997, P<0.001) for AccuFFRivus and 0.897 (95%CI:0.740-0.974, P<0.05) for AccuFFRct. A critical value of 0.80 for AccuFFRivus predicted FFR≤0.80 with high sensitivity (96.6%), specificity (85.7%), and the Youden index (0.823). AccuFFRct with predicted FFR≤0.80 demonstrated good diagnostic performance (sensitivity 89.7%, specificity 85.7% and Youden index 0.754).
Conclusions: IVUS-FFR may also become a new clinically meaningful functional evaluation method for diagnosing myocardial ischemia and may be as important as CT-FFR, Angio-FFR, OCT-FFR, and invasive FFR in the future as a method for evaluating coronary artery disease.