bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Profiling of Immune Cell Types in Peripheral Blood Reveals Common and Specific Pathways Involved in the Pathogenesis of Myositis‐Specific Antibody‐Positive Inflammatory Myopathies
Authors: Sugimori Yusuke , Iwasaki Yukiko , Takeshima Yusuke , Okubo Mai , Kobayashi Satomi , Hatano Hiroaki , Yamada Saeko , Nakano Masahiro , Yoshida Ryochi , Ota Mineto , Tsuchida Yumi , Nagafuchi Yasuo , Shimane Kenichi , Yoshida Ken , Kurosaka Daitaro , Sumitomo Shuji , Shoda Hirofumi , Yamamoto Kazuhiko , Okamura Tomohisa , Fujio Keishi 
Published: January 2023

ObjectiveIdiopathic inflammatory myopathies (IIM) demonstrate characteristic clinical phenotypes depending on the myositis‐specific antibody (MSAs) present. We aimed to identify common or MSA‐specific immunological pathways in different immune cell types from peripheral blood by transcriptome analysis.MethodsWe recruited 33 patients with IIM who were separated into the following groups: 15 patients with active disease at onset and 18 with inactive disease under treatment. All patients were positive for MSAs: anti–melanoma differentiation‐associated gene 5 (MDA5) antibody (Ab) in 10 patients, anti‐Mi‐2 Ab in 7, and anti‐aminoacyl‐transfer RNA synthetase (ARS) Ab in 16. The patients were compared with 33 healthy controls. Twenty‐four immune cell types sorted from peripheral blood were analyzed by flow cytometry, RNA sequencing, and differentially expressed gene analysis combined with pathway analysis.ResultsThe frequencies of memory B cell types were significantly decreased in active patients, and the frequency of plasmablasts was prominently increased in active patients with anti‐MDA5 Ab in comparison with healthy controls. The expression of type I interferon (IFN)‐stimulated genes of all immune cell types was increased in the active, but not inactive, patients. Endoplasmic reticulum stress‐related genes in all IIM memory B cells and oxidative phosphorylation‐related genes in inactive IIM double negative B cells were also increased, suggesting prominent B cell activation in IIM. Furthermore, active patients with anti‐MDA5 Ab, anti‐Mi‐2 Ab, or anti‐ARS Ab were distinguished by IFN‐stimulated and oxidative phosphorylation‐related gene expression in plasmablasts.ConclusionUnique gene expression patterns in patients with IIM with different disease activity levels and MSA types suggest different pathophysiologies. Especially, B cells may contribute to common and MSA‐specific immunological pathways in IIM.
Dopamine‐Integrated Nanointerface between Fibrillar Matrix and Hydrophilic Nanohydroxyapatite Regulates Immune Microenvironment to Boost Endogenous Bone Regeneration
Authors: Li Xing , Li Zhulian , Wang Peilei , Lu Gonggong , Tong Lei , Liu Quanying , Chen Yafang , Lin Jiangli , Luo En , Liang Jie , Jiang Qing , Fan Yujiang , Zhang Xingdong , Sun Yong 
Published: January 2023

AbstractDriving endogenous bone regeneration by cell‐ and factor‐free biomaterials is the most ideal repair strategy. Herein, hybrid interleaved scaffold (HDSH) with nanosized interfacial integration is assembled by organic/inorganic interactive bonding at the nanoscale. With the help of transcriptome and proteome analysis, the regenerative mechanism of this scaffold is elaborated at the molecular level, which confirms that this strategy recreates a suitable immune microenvironment (anti‐inflammatory and M2‐polarizing) and drives functional cell and cytokine adhesion, as well as inchoate vascularization. It greatly enhances endogenous stem cell recruitment, and subsequently initiates robust vasculogenesis and osteogenesis. Significant bony reconstitution in the rabbit cranial defect model (Φ = 10 mm) is observed after 12 weeks, which realizes completely new bone coverage and 79% breaking load strength relative to the natural cranium. By enhancing nano‐sized functional interfacial integration, this strategy can provide effective guidance for developing highly bioactive bone‐regenerative implants.
Microphysiological Drug‐Testing Platform for Identifying Responses to Prodrug Treatment in Primary Leukemia
Authors: Gökçe Furkan , Kaestli Alicia , Lohasz Christian , de Geus Martina , Kaltenbach Hans‐Michael , Renggli Kasper , Bornhauser Beat , Hierlemann Andreas , Modena Mario 
Published: January 2023

AbstractDespite increasing survival rates of pediatric leukemia patients over the past decades, the outcome of some leukemia subtypes has remained dismal. Drug sensitivity and resistance testing on patient‐derived leukemia samples provide important information to tailor treatments for high‐risk patients. However, currently used well‐based drug screening platforms have limitations in predicting the effects of prodrugs, a class of therapeutics that require metabolic activation to become effective. To address this issue, a microphysiological drug‐testing platform is developed that enables co‐culturing of patient‐derived leukemia cells, human bone marrow mesenchymal stromal cells, and human liver microtissues within the same microfluidic platform. This platform also enables to control the physical interaction between the diverse cell types. Herein, it is made possible to recapitulate hepatic prodrug activation of ifosfamide in their platform, which is very difficult in traditional well‐based assays. By testing the susceptibility of primary patient‐derived leukemia samples to the prodrug ifosfamide, sample‐specific sensitivities to ifosfamide in primary leukemia samples are identified. The microfluidic platform is found to enable the recapitulation of physiologically relevant conditions and the testing of prodrugs including short‐lived and unstable metabolites. The platform holds great potential for clinical translation and precision chemotherapy selection.
Photoreaction Boosting Phosphorescence Cascade Energy Transfer Based on Cucurbit[8]Uril Biaxial Polypseudorotaxane
Authors: Xing Wen‐Wen , Wang Hui‐Juan , Liu Zhixue , Yu Zhen‐Hai , Zhang Heng‐Yi , Liu Yu 
Published: January 2023

AbstractCascade energy capturers based on supramolecular assembly have recently generated great research interest in the field of luminescent materials. Herein is reported a cucurbit[8]uril (CB[8])‐encapsulated coumarin‐bridged phenylpyridinium salt (G) to form biaxial polypseudorotaxane (G⊂CB[8]), which not only induces the phosphorescence of the guest G, but also activates the photodimerization of coumarin functional group in the CB[8] cavity (G‐dimer⊂CB[8]) to further boost its phosphorescence emission, extending the phosphorescence lifetime from 271.3 to 430.3 µs and the quantum yield from 5.1% to 25.7% in aqueous solution. After co‐assembly with amphiphilic calixarene (SC4AD), the supramolecular biaxial polypseudorotaxane G‐dimer⊂CB[8] is changed to the nanoparticle. Compared with the alone G‐dimer⊂CB[8], the phosphorescence intensity of G‐dimer⊂CB[8]@SC4AD is significantly enhanced with the phosphorescence lifetime extending to 2.11 ms. Subsequently, by doping commercial dye rhodamine B (RhB) in the G‐dimer⊂CB[8]@SC4AD assembly, an ultrahighly efficient phosphorescence‐harvesting energy transfer process occurs with an energy transfer efficiency (ΦET) of 72.3% and an antenna effect of 396.8. Furthermore, the cascaded room temperature phosphorescence harvesting system is constructed via introducing the secondary near‐infrared (NIR) acceptors Nile blue or Cy5 in the G‐dimer⊂CB[8]@SC4AD:RhB system, which possesses a good biocompatibility and is successfully applied to NIR delayed fluorescence targeted imaging in HeLa cell.
A Space‐Dependent ‘Enzyme‐Substrate’ Type Probe based on ‘Carboxylesterase‐Amide Group’ for Ultrafast Fluorescent Imaging Orthotopic Hepatocellular Carcinoma
Authors: Wen Ying , Jing Ning , Zhang Min , Huo Fangjun , Li Zhuoyu , Yin Caixia 
Published: January 2023

AbstractFast and selective fluorescence imaging for a biomarker to related‐disease diagnosis remains a significant challenge due to complex physical environment. Human carboxylesterase (CE) is expected to be a potential biomarker of hepatocellular carcinoma (HCC) to improve the accuracy of diagnosis. However, existing probes for CE has slow response rate and low selectivity. Herein, the amide group is selected as CE‐responsive sites based on the “substrate‐hydrolysis enzymatic reaction” approach. From a series of off–on probes with leave groups in the amide unit, probe JFast is screened with the optimal combination of rapid response rate and high selectivity toward CE. JFast requires only 150 s to reach the maximum fluorescence at 676 nm in the presence of CE and free from the interference of other esterase. Computational docking simulations indicate the shortest distance between the CE and active site of JFast. Cell and in vivo imaging present that the probe can turn on the liver cancer cells and tumor region precisely. Importantly, JFast is allowed to specifically image orthotopic liver tumor rather than metastatic tumor and distinguish human primary liver cancer tissue from adjacent ones. This study provides a new tool for CE detection and promotes advancements in accurate HCC diagnosis.
The relationship between case–control differential gene expression from brain tissue and genetic associations in schizophrenia
Authors: Clifton Nicholas E. , Schulmann Anton ,   Schizophrenia Working Group of the Psychiatric Genomics Consortium, Holmans Peter A. , O'Donovan Michael C. , Vawter Marquis P. 
Published: January 2023

AbstractLarge numbers of genetic loci have been identified that are known to contain common risk alleles for schizophrenia, but linking associated alleles to specific risk genes remains challenging. Given that most alleles that influence liability to schizophrenia are thought to do so by altered gene expression, intuitively, case–control differential gene expression studies should highlight genes with a higher probability of being associated with schizophrenia and could help identify the most likely causal genes within associated loci. Here, we test this hypothesis by comparing transcriptome analysis of the dorsolateral prefrontal cortex from 563 schizophrenia cases and 802 controls with genome‐wide association study (GWAS) data from the third wave study of the Psychiatric Genomics Consortium. Genes differentially expressed in schizophrenia were not enriched for common allelic association statistics compared with other brain‐expressed genes, nor were they enriched for genes within associated loci previously reported to be prioritized by genetic fine‐mapping. Genes prioritized by Summary‐based Mendelian Randomization were underexpressed in cases compared to other genes in the same GWAS loci. However, the overall strength and direction of expression change predicted by SMR were not related to that observed in the differential expression data. Overall, this study does not support the hypothesis that genes identified as differentially expressed from RNA sequencing of bulk brain tissue are enriched for those that show evidence for genetic associations. Such data have limited utility for prioritizing genes in currently associated loci in schizophrenia.
Visible‐Light Induced Oxidative Annulation of Enamides to 2,4,5‐Trisubstituted Oxazole Skeletons
Authors: Wang Dan‐Ning , Lin Qi , Wang Xun , Zhang Keyuan , Duan Xin‐Hua , Liu Le 
Published: January 2023

AbstractA novel approach to various trisubstituted oxazoles (28 examples, up to 88% yield) has been developed via 9H‐thioxanthen‐9‐one (TX) mediated oxidative annulation of N‐acetyl enamide under visible light irradiation in the presence of BF3 ⋅ Et2O (2.0 equiv) and 40 mol% of PhNO2. This metal‐free procedure features high atom and step economy, green and mild conditions and broad substrate scope.
Superior conductive polypyrrole anti‐corrosion coating doped with titanium nitride for 304 stainless steel bipolar plates
Authors: Ding Guoxin , Wang Chen , Gao Nan , Li Ruofan , Wang Yunpeng , Ma Haitao 
Published: January 2023

AbstractThe conductivity and corrosion performance of metallic bipolar plates were crucial to the development of the proton exchange membrane fuel cell (PEMFC). In this study, polypyrrole (PPy) coatings doped with titanium nitride nanoparticles (TiN NPs) were deposited on 304 stainless steel (304SS) by constant potential polymerization. To improve dispersion and form a negative charge surface, TiN NPs were modified by anionic surfactants such as sodium dodecyl diphenyl ether disulfate (MADS). Through comparing potentiodynamic curves of the composite coatings in the simulated cathodic environment of PEMFC, the PPy‐0.1 TiN (0.1 g/L) coating presented a more positive corrosion potential (Ecorr = 293 mVAg/AgCl) and a lower corrosion current (Icorr = 0.841 μA cm−2). Furthermore, PPy‐0.1 TiN exhibited more stable corrosion resistance during long‐term immersion. Interfacial contact resistance (ICR) testing demonstrated that the addition of TiN NPs improved conductivity compared to a PPy coating. The results indicated that the PPy‐TiN‐coated 304SS could be used as a kind of promising bipolar plate material.
The neuropeptide substance P/neurokinin‐1 receptor system and diabetes: From mechanism to therapy
Authors: Kokabi Fariba , Ebrahimi Safieh , Mirzavi Farshad , Ghiasi Nooghabi Nazanin , Hashemi Seyedeh Fatemeh , Hashemy Seyed Isaac 
Published: January 2023

AbstractDiabetes is a significant public health issue known as the world's fastest‐growing disease condition. It is characterized by persistent hyperglycemia and subsequent chronic complications leading to organ dysfunction and, ultimately, the failure of target organs. Substance P (SP) is an undecapeptide that belongs to the family of tachykinin (TK) peptides. The SP‐mediated activation of the neurokinin 1 receptor (NK1R) regulates many pathophysiological processes in the body. There is also a relation between the SP/NK1R system and diabetic processes. Importantly, deregulated expression of SP has been reported in diabetes and diabetes‐associated chronic complications. SP can induce both diabetogenic and antidiabetogenic effects and thus affect the pathology of diabetes destructively or protectively. Here, we review the current knowledge of the functional relevance of the SP/NK1R system in diabetes pathogenesis and its exploitation for diabetes therapy. A comprehensive understanding of the role of the SP/NK1R system in diabetes is expected to shed further light on developing new therapeutic possibilities for diabetes and its associated chronic conditions.
Insights into quasi solid‐state polymer electrolyte: The influence of succinonitrile on polyvinylene carbonate electrolyte in view of electrochemical applications
Authors: Liu Zelin , Zhang Shu , Zhou Qian , Zhang Yidong , Lv Dong , Shen Yanghuan , Fu Xiangnan , Wang Xinyi , Luo Shiyu , Zheng Yun , Peng Yu , Chai Jingchao , Liu Zhihong , Cui Guanglei 
Published: January 2023

AbstractQuasi solid‐state composite polymer electrolytes have generated much interest due to their high ionic conductivity and stable interfacial compatibility with electrodes. However, they suffer from the balance of liquid plasticizer content and ionic conductivity to retard potential safety issues. In this paper, a polyvinylene carbonate (PVCA)‐based quasi solid‐state composite polymer electrolyte for ambient‐temperature lithium‐ion battery is proposed, wherein succinonitrile (SN) serves as the multifunctional component to increase the ionic conductivity. The study indicates that SN can plasticize the polymer electrolyte, coordinate with lithium‐ion, and modulate the molecular weight of the polymer. The fabricated PVCA–SN polymer electrolyte delivers an ionic conductivity up to 4.5 × 10–4 S/cm at 25°C. The assembled lithium‐ion battery based on PVCA electrolyte demonstrates an excellent rate performance and high‐capacity retention at ambient temperature. The proposition of PVCA‐based quasi solid‐state composite polymer electrolyte provides a resultful strategy for high‐performance rechargeable lithium batteries.
Third‐line or above anlotinib in relapsed and refractory small cell lung cancer patients with brain metastases: A post hoc analysis of ALTER1202, a randomized, double‐blind phase 2 study
Authors: Cheng Ying , Wang Qiming , Li Kai , Shi Jianhua , Han Baohui , Wu Lin , Chen Gongyan , He Jianxing , Wang Jie , Qin Haifeng , Li Xiaoling 
Published: January 2023

AbstractBackgroundThe prognosis of patients with small cell lung cancer (SCLC) and brain metastases (BM) was poor. This study aimed to explore the efficacy and safety of anlotinib as third‐line or above treatment in SCLC with BM.MethodsThis was a subgroup analysis of the ALTER1202 trial, which was a randomized, placebo‐controlled trial aimed to evaluate the role of anlotinib as third‐line treatment or above in patients with SCLC. This study included patients with BM at baseline. The efficacy and safety outcomes included progression‐free survival (PFS), overall survival (OS), central nervous system (CNS), objective response rate (ORR), CNS disease control rate (DCR), time to CNS progression, and adverse events (AEs).ResultsTwenty‐one and nine patients with BM were included in the anlotinib and placebo groups, respectively. The median PFS and OS were 3.8 months (95% confidence interval [CI]: 1.8–6.1) and 6.1 months (95% CI: 4.1–8.0) in the anlotinib group. Anlotinib was associated with a significant improvement in PFS (hazard ratio [HR] = 0.15, 95% CI: 0.04–0.51, p = 0.0005) and OS (HR = 0.26, 95% CI: 0.09–0.73, p = 0.0061) than placebo. Anlotinib significantly prolonged the time to CNS progression (p < 0.0001). The anlotinib group had a higher CNS DCR than placebo (95.2% vs. 22.2%, p = 0.0001). The most common grade 3 or higher AEs were increased lipase (19.0%), hypertension (14.3%), and hyponatremia (14.3%) in the anlotinib group.ConclusionsAnlotinib proved to have potential CNS activity and a manageable toxicity profile in patients with SCLC and BM, significantly delaying CNS progression.
Survival nomograms for simultaneous resection of primary and hepatic lesions without neoadjuvant chemotherapy in patients with resectable colorectal liver metastasis
Authors: Jiang Yu‐Juan , Zhou Si‐Cheng , Zhu Zi‐Xing , Chen Jing‐Hua , Liang Jian‐Wei 
Published: January 2023

AbstractBackgroundNo well‐performing nomogram has been developed specifically to predict individual‐patient cancer‐specific survival (CSS) and overall survival (OS) among patients with resectable colorectal liver metastasis (CRLM) who undergo simultaneous resection of primary and hepatic lesions without neoadjuvant chemotherapy (NAC). We aim to investigate the prognosis of patients with resectable CRLM undergoing simultaneous resection of primary and hepatic lesions without NAC.MethodsData of patients with CRLM in the Surveillance, Epidemiology and End Results Program (cohort, n = 225) were collected as the training set, and data of patients with CRLM treated at the National Cancer Center (cohort, n = 180) were collected as the validation set. The prognostic value of the clinicopathological parameters in the training cohort was assessed using Kaplan‒Meier curves and univariate and multivariate Cox proportional hazards models, and OS and CSS nomograms integrated with the prognostic variables were constructed. Calibration analyses, receiver operating characteristic (ROC) curves, and decision curve analyses (DCAs) were then performed to evaluate the performance of the nomograms.ResultsThere was no collinearity among the collected variables. Three factors were associated with OS and CSS: the pretreatment carcinoembryonic antigen (CEA) concentration, pathologic N (pN) stage, and adjuvant chemotherapy (each p < 0.05). OS and CSS nomograms were constructed using these three parameters. The calibration plots revealed favorable agreement between the predicted and observed outcomes. The areas under the ROC curves were approximately 0.7. The DCA plots revealed that both nomograms had satisfactory clinical benefits. The ROC curves and DCAs also confirmed that the nomogram surpassed the tumor, node, and metastasis staging system.ConclusionThe herein‐described nomograms containing the pretreatment CEA concentration, pN stage, and adjuvant chemotherapy may be effective models for predicting postoperative survival in patients with CRLM.
Comprehensive immunoprofile analysis of prognostic markers in pancreaticobiliary tract cancers
Authors: Kim Ji Eun , Kim Hyemin , Kim Binnari , Chung Hye Gyo , Chung Hwe Hoon , Kim Kyoung Mee , Kim Seong Hyun , Jeong Woo Kyoung , Kim Young Kon , Min Ji Hye , Heo Jin Seok , Han In Woong , Shin Sang Hyun , Park Hee Chul , Yu Jeong Il , Park Joon Oh , Kim Seung Tae , Hong Jung Yong , Lee Se‐Hoon , Lee Kwang Hyuck , Lee Jong Kyun , Lee Kyu Taek , Jang Kee‐Taek , Park Joo Kyung 
Published: January 2023

AbstractPancreaticobiliary tract cancer has a poor prognosis with unmet needs in a new target treatment. Some studies have reported that an enhancement of T‐cell immunity is associated with a good prognosis. The aim of this study is to investigate the immunoprofile as a prognostic marker of pancreaticobiliary tract cancers. Unresectable pancreatic ductal adenocarcinoma (PDAC, n = 80) and biliary tract cancer (BTC, n = 74) diagnosed between January 2012 and December 2018 in Samsung Medical Center were analyzed. Expression levels of CD8, FOXP3, PD‐1, PD‐L1, and CXCL13 in PDAC and BTC tissue samples were examined with immunohistochemical staining, which was evaluated with various clinical factors. In PDAC, higher degree of PD‐L1 expression was significantly associated with shorter overall survival (OS) (p = 0.0095). On the other hand, higher infiltrations of PD‐1+ immune cells (p = 0.0002) and CD8+ T cells (p = 0.0067) were associated with longer OS. In BTC, higher FOXP3+ (p = 0.0343) and CD8+ (p = 0.0028) cell infiltrations were associated with better survival. Low infiltration of CD8+ (p = 0.0148), FOXP3+ (p = 0.0208), PD‐1+ (p = 0.0318) cells in PDAC, and FOXP3+ cells (p = 0.005) in BTC were considerably related to metastasis. In a combined evaluation of clinical factors and immunoprofiles, univariate analysis revealed that operation after chemotherapy (p < 0.0001), mass size (p = 0.0004), metastasis (p = 0.006), PD‐L1 (p < 0.0001), PD‐1 (p = 0.003) and CD8 (p = 0.0063) was significantly associated with OS in PDAC, and CD8 (p = 0.007) was statistically related to OS in BTC. In multivariate analysis, prognostic factors were operation after chemotherapy (p = 0.021) in PDAC and CD8 (p = 0.037) in BTC. Therefore, immunoprofile analysis of cells expressing CD8, FOXP3, PD‐1, and PD‐L1 might have prognostic values in patients with pancreaticobiliary tract cancers.