bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi‐omics data reveals novel impacts of human papillomavirus integration on the epigenomic and transcriptomic signatures of cervical tumorigenesis
Authors: Zeng Xi , Wang Yuyouye , Liu Binghan , Rao Xinjie , Cao Canhui , Peng Fang , Zhi Wenhua , Wu Ping , Peng Ting , Wei Ye , Chu Tian , Xu Miaochun , Xu Yashi , Ding Wencheng , Li Guoliang , Lin Shitong , Wu Peng 
Published: May 2023

AbstractIntegration of human papilloma virus (HPV) DNA into the human genome may progressively contribute to cervical carcinogenesis. To explore how HPV integration affects gene expression by altering DNA methylation during carcinogenesis, we analyzed a multiomics dataset for cervical cancer. We obtained multiomics data by HPV‐capture sequencing, RNA sequencing, and Whole Genome Bisulfite Sequencing from 50 patients with cervical cancer. We detected 985 and 485 HPV‐integration sites in matched tumor and adjacent paratumor tissues. Of these, LINC00486 (n = 19), LINC02425 (n = 11), LLPH (n = 11), PROS1 (n = 5), KLF5 (n = 4), LINC00392 (n = 3), MIR205HG (n = 3) and NRG1 (n = 3) were identified as high‐frequency HPV‐integrated genes, including five novel recurrent genes. Patients at clinical stage II had the highest number of HPV integrations. E6 and E7 genes of HPV16 but not HPV18 showed significantly fewer breakpoints than random distribution. HPV integrations occurring in exons were associated with altered gene expression in tumor tissues but not in paratumor tissues. A list of HPV‐integrated genes regulated at transcriptomic or epigenetic level was reported. We also carefully checked the candidate genes with regulation pattern correlated in both levels. HPV fragments integrated at MIR205HG mainly came from the L1 gene of HPV16. RNA expression of PROS1 was downregulated when HPV integrated in its upstream region. RNA expression of MIR205HG was elevated when HPV integrated into its enhancer. The promoter methylation levels of PROS1 and MIR205HG were all negatively correlated with their gene expressions. Further experimental validations proved that upregulation of MIR205HG could promote the proliferative and migrative abilities of cervical cancer cells. Our data provides a new atlas for epigenetic and transcriptomic regulations regarding HPV integrations in cervical cancer genome. We demonstrate that HPV integration may affect gene expression by altering methylation levels of MIR205HG and PROS1. Our study provides novel biological and clinical insights into HPV‐induced cervical cancer.
Mechanistic insights into the role of actinobacteria as potential biocontrol candidates against fungal phytopathogens
Authors: Kaur Talwinder , Khanna Kanika , Sharma Sonika , Manhas Rajesh K. 
Published: May 2023

AbstractWorldwide mounting demand for better food production to nurture exasperating population emphasizes on reduced crop losses. The incidence of pathogens into the agricultural fields has tend to dwindle plethora of cereal, vegetable, and other fodder crops. This, in turn, has seriously impacted the economic losses on global scale. Apart from this, it is quite challenging to feed the posterity in the coming decades. To counteract this problem, various agrochemicals have been commercialized in the market that no doubt shows positive results but along with adversely affecting the ecosystem. Therefore, the excessive ill‐fated use of agrochemicals to combat the plant pests and diseases highlights that alternatives to chemical pesticides are need of the hour. In recent days, management of plant diseases using plant‐beneficial microbes is gaining interest as safer and potent alternatives to replace chemically based pesticides. Among these beneficial microbes, actinobacteria especially streptomycetes play considerable role in combating plant diseases along with promoting the plant growth and development along with their productivity and yield. The mechanisms exhibited by actinobacteria include antibiosis (antimicrobial compounds and hydrolytic enzymes), mycoparasitism, nutrient competition, and induction of resistance in plants. Thus, in cognizance with potential of actinobacteria as potent biocontrol agents, this review summarizes role of actinobacteria and the multifarious mechanisms exhibited by actinobacteria for commercial applications.
Spring snowmelt variations over the Tibetan Plateau and its influences on spring and summer precipitation
Authors: Zhou Juan , Wen Jun , Yang Xianyu , Lai Xin , Li Xiao , Jian Dongnan 
Published: May 2023

AbstractBoth snow and precipitation are vital components of the water cycle and hydrological system, so the snow–precipitation feedback could be crucial for investigating land–atmosphere interaction and climate variability. Seldom studies have focused on the contributions of snow variations to precipitation anomalies over the Tibetan Plateau (TP). In this study, spring snowmelt (SSD, Spring Snow water equivalent Differences) variations over the TP and its influences on spring and summer precipitation and the associated physical mechanisms during 1979–2014 are investigated. Results show that the leading mode of the interannual SSD over the TP exhibits a same‐sign anomaly pattern, with an intense centre over the western TP. The SSD shows significant relations to spring and summer precipitation over the TP. In spring, when larger SSD appears over the western TP, an anomalous cyclone occupies the northwestern TP, and the central and eastern TP lies between an anomalous cyclone and an anomalous anticyclone. There is an anomalous convergence (divergence) and ascending (descending) motion of airflows over the northwestern (central and eastern) TP. Concurrently, sufficient moisture occurs over the northwestern TP due to evaporation and atmospheric circulations. As a result, precipitation anomalously increases over the northwestern TP, and decreases over the central and southeastern TP. The SSD‐related soil moisture anomalies over the western TP can persist into summer, and larger SSD corresponds to excessive summer soil moisture. The central and eastern TP lies between two anticyclones, leading to convergence of airflows and thereby ascending motions. Simultaneously, excessive moisture appears over the TP owing to atmospheric circulation transportation. Hence, precipitation anomalously increases over the central and eastern TP.
Metabolomics reveals the role of Lactobacillus plantarumSHY130 in hepatic metabolic regulation in a mouse model of type 2 diabetes
Authors: Wang Guangqi , Song Jiajia , Wang Chen , Chen Xiaoyong , Suo Huayi 
Published: May 2023

AbstractBACKGROUNDAmong type 2 diabetes (T2D) patients, the incidence rate of liver metabolic disorders is much higher than that in healthy subjects. It was observed in our previous research that diabetic symptoms were improved by Lactobacillus plantarum SHY130 (LPSHY130) isolated from yak yogurt in a murine model of T2D. This study sought to investigate the LPSHY130‐mediated hepatic metabolic regulation in a murine model of T2D.RESULTSTreatment with LPSHY130 improved liver function and pathological damage in diabetic mice. Untargeted metabolome analysis revealed that T2D‐induced changes in 11 metabolites were regulated after LPSHY130 treatment, mainly involving purine metabolism, amino acid metabolism, and choline metabolism and pantothenate and coenzyme A biosynthesis pathways. In addition, correlation analysis indicated that hepatic metabolic changes can be adjusted by the intestinal microbiota.CONCLUSIONOverall, this study suggests that treatment with LPSHY130 relieves liver injury and regulates liver metabolism in a murine model of T2D, thus providing a theoretical basis for the use of probiotics as dietary supplements to regulate hepatic metabolic disorders associated with T2D. © 2023 Society of Chemical Industry.
Genome‐wide association studies reveal novel loci controlling tuber flesh color and oxidative browning in Dioscorea alata
Authors: Dossa Komivi , Morel Angélique , Houngbo Mahugnon Ezékiel , Mota Ana Zotta , Malédon Erick , Irep Jean‐Luc , Diman Jean‐Louis , Mournet Pierre , Causse Sandrine , Van Kien Nguyen , Cornet Denis , Chair Hâna 
Published: May 2023

AbstractBACKGROUNDConsumers' preferences for food crops are guided by quality attributes. This study aimed at deciphering the genetic basis of quality traits, especially tuber flesh color (FC) and oxidative browning (OB) in Dioscorea alata, based on the genome‐wide association studies (GWAS) approach. The D. alata panel was planted at two locations in Guadeloupe. At harvest, the FC was scored visually as white, cream, or purple on longitudinally sliced mature tubers. The OB was scored visually as the presence or absence of browning after 15 min of exposure of the sliced samples to ambient air.RESULTSPhenotypic characterization for FC and OB of a diverse panel of D. alata genotypes highlighted significant variation within the panel and across two locations. The genotypes within the panel displayed a weak structure and could be classified into three subpopulations. GWAS identified 14 and 4 significant associations for tuber FC and OB, respectively, with phenotypic variance, explained values ranging from 7.18% to 18.04%. Allele segregation analysis at the significantly associated loci highlighted the favorable alleles for the desired traits, i.e., white FC and no OB. A total of 24 putative candidate genes were identified around the significant signals. A comparative analysis with previously reported quantitative trait loci indicated that numerous genomic regions control these traits in D. alata.CONCLUSIONOur study provides important insights into the genetic control of tuber FC and OB in D. alata. The major and stable loci can be further utilized to improve selection in breeding programs for developing new cultivars with enhanced tuber quality. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Cerebrovascular Phenotype in Fabry Disease Patients Assessed by Ultrasound
Authors: Rekova Petra , Dostalova Gabriela , Rob Daniel , Vaneckova Manuela , Pavlicova Martina , Linhart Ales , Kemlink David 
Published: May 2023

ObjectivesFabry disease (FD) is a rare X‐linked lysosomal storage disorder with variable phenotypes, including neurological symptoms. These can be influenced by vascular impairment. Extracranial and transcranial vascular sonography is an effective and noninvasive method for measuring arterial structures and blood flow.The study aims to investigate cerebrovascular phenotype characteristics in FD patients compared to controls using neurosonology.MethodsThis is a single‐center, cross‐sectional study of 130 subjects—65 patients (38 females), with genetically confirmed FD, and 65 sex‐ and age‐matched controls. Using ultrasonography, we measured structural and hemodynamic parameters, including distal common carotid artery intima‐media thickness, inner vertebral artery diameter, resting blood flow velocity, pulsatility index, and cerebral vasoreactivity (CVR) in the middle cerebral artery. To assess differences between FD and controls and to identify factors influencing investigated outcomes, unadjusted and adjusted regression analyses were performed.ResultsIn comparison to sex‐ and age‐matched controls, FD patients displayed significantly increased carotid artery intima‐media thickness (observed FD 0.69 ± 0.13 mm versus controls 0.63 ± 0.12 mm; Padj = .0014), vertebral artery diameter (observed FD 3.59 ± 0.35 mm versus controls 3.38 ± 0.33 mm; Padj = .0002), middle cerebral artery pulsatility index (observed FD 0.98 ± 0.19 versus controls 0.87 ± 0.11; Padj < .0001), and significantly decreased CVR (observed FD 1.21 ± 0.49 versus controls 1.35 ± 0.38; Padj = .0409), when adjusted by age, BMI, and sex. Additionally, FD patients had significantly more variable CVR (0.48 ± 0.25 versus 0.21 ± 0.14; Padj < .0001).ConclusionsOur results suggest the presence of multiple vascular abnormalities and changes in hemodynamic parameters of cerebral arteries in patients with FD.
A fast and efficient tool for the structural characterization of marine dissolved organic matter: Nonuniform sampling 2D COSY NMR
Authors: Vemulapalli Sahithya Phani Babu , Griesinger Christian , Dittmar Thorsten 
Published: May 2023

AbstractAn in‐depth structural characterization of marine dissolved organic matter (DOM) is crucial for a better understanding of its connection to marine and global biogeochemical cycles. High‐field nuclear magnetic resonance (NMR) spectroscopy in general and two‐dimensional (2D) correlation spectroscopy (COSY) in particular are powerful tools for the molecular level structural analysis of marine DOM. These 2D NMR experiments demand prolonged experimental times of days to weeks per sample due to the requirement of a large number of experiments to record the second dimension (t1) of the 2D NMR experiment. Herein, we demonstrate the efficacy of nonuniform sampling (NUS) in 2D COSY, which (i) reduces the measurement time by half without compromising spectral quality and (ii) enhances the signal intensity for the given experiment time. This approach can lead to substantial progress in the structural analysis of previously poorly characterized marine DOM. NUS COSY has been exemplified on two solid‐phase extracted DOM samples from the surface and deep ocean at 800 MHz and 1.2 GHz instruments. A dramatic improvement in sensitivity and spectral resolution is observed in NUS COSY spectra recorded at 1.2 GHz instrument when compared to 800 MHz instrument. NUS COSY NMR is versatile and anticipated to have significant potential for uncovering the hidden molecular diversity of DOM from various aquatic environments within a reasonable timeframe. The introduction of NUS into the environmental sciences was long overdue, and our study now opens the door for a wide field of new applications of NMR in the marine and aquatic sciences.
Opening Pores and Extending the Application Window: Open‐Cell Nanocellular Foams
Authors: Cuadra‐Rodríguez Daniel , Barroso‐Solares Suset , Laguna‐Gutiérrez Ester , Rodríguez‐Pérez Miguel Ángel , Pinto Javier 
Published: May 2023

AbstractThe production of open‐cell (OC) nanostructures in polymer foams without non‐foamed solid skins by gas dissolution foaming has been developed in this work. First, several grades of MAM block copolymer (methyl methacrylate‐b‐butyl acrylate‐b‐methyl methacrylate) at high content are employed as heterogeneous phase in poly(methyl methacrylate) for producing OC structures. Atomic force microscopy and extensional rheology are used as methods to understand the main features to obtain OC nanocellular structures. Second, the gas diffusion barrier approach is employed for the first time in polymer blends to avoid the appearance of the solid skins in the borders, which typically appears when the cellular polymer is produced by gas dissolution foaming. The influence of the poly(vinyl alcohol) gas diffusion barrier is analyzed, together with the effect of heterogeneous nucleation provided by MAM copolymer, on the solid skins’ formation. The synergy between both approaches allows obtaining porous nanocellular polymeric films with an OC structure non‐constrained by the presence of outer solid skins.
An Intriguing Array of Extrudate Patterns in Long‐Chain Branched Polymers During Extrusion
Authors: Pashazadeh Sajjad , Aulova Alexandra , Georgantopoulos Christos K. , Bek Marko , Vittorias Iakovos , Naue Ingo F.C. , Wilhelm Manfred , Kádár Roland 
Published: May 2023

AbstractThe present study highlights a range of surface and volume extrudate patterns that can be detected during the extrusion flow of long‐chain branched polymers. Thus, four linear low‐density polyethylenes (LDPEs) have been extruded using a single‐screw extruder coupled to an inline optical imaging system. The selected LDPEs are selected to outline the influence of molecular weight and long‐chain branching on the types of melt flow extrusion instabilities (MFEI). Through the inline imaging system, space–time diagrams are constructed and analyzed via Fourier‐transformation using a custom moving window procedure. Based on the number of characteristic frequencies, peak broadness, and whether they are surface or volume distortions, three main MFEI types, distinct from those typically observed in linear and short‐chain branched polymers, are identified. The higher molecular weight, low long‐chain branching LDPEs exhibited all three instability types, including a special type volume instability. Independently of the molecular weight, higher long‐chain branching appeared to have a stabilizing effect on the transition sequences by suppressing volume extrudate distortions or limiting surface patters to a form of weak intensity type.
Gevrey semiglobal solvability for a class of elliptic vector fields with degeneracies
Authors: Araújo Gabriel , Bergamasco Adalberto P. , Dattori da Silva Paulo L. 
Published: May 2023

AbstractWe deal with Gevrey solvability of a class of complex vector fields defined on , given by , , near the characteristic set . Diophantine conditions appear in a natural way in our results.
Theorems of Wolff–Denjoy type for semigroups of nonexpansive mappings in geodesic spaces
Authors: Huczek Aleksandra , Wiśnicki Andrzej 
Published: May 2023

AbstractWe show that if is a one‐parameter continuous semigroup of nonexpansive mappings acting on a complete locally compact geodesic space that satisfies some geometric properties, then there exists such that S converge uniformly on bounded sets of Y to ξ. In particular, our result applies to strictly convex bounded domains in or with respect to a large class of metrics including Hilbert's and Kobayashi's metrics.
Regularity results for Choquard equations involving fractional p‐Laplacian
Authors: Biswas Reshmi , Tiwari Sweta 
Published: May 2023

AbstractIn this paper, first we address the regularity of weak solution for a class of p‐fractional Choquard equations:
where is a smooth bounded domain, and such that , and is a continuous function with at most critical growth condition (in the sense of the Hardy–Littlewood–Sobolev inequality) and F is its primitive. Next, for , we discuss the Sobolev versus Hölder minimizers of the energy functional J associated with the above problem, and using that we establish the existence of the local minimizer of J in the fractional Sobolev space . Moreover, we discuss the aforementioned results by adding a local perturbation term (at most critical in the sense of Sobolev inequality) in the right‐hand side in the above equation.
Slowing of Frontal β Oscillations in Atypical Parkinsonism
Authors: Krösche Marius , Kannenberg Silja , Butz Markus , Hartmann Christian J. , Florin Esther , Schnitzler Alfons , Hirschmann Jan 
Published: May 2023

AbstractBackgroundDiagnosis of atypical parkinsonian syndromes (APS) mostly relies on clinical presentation as well as structural and molecular brain imaging. Whether parkinsonian syndromes are distinguishable based on neuronal oscillations has not been investigated so far.ObjectiveThe aim was to identify spectral properties specific to atypical parkinsonism.MethodsWe measured resting‐state magnetoencephalography in 14 patients with corticobasal syndrome (CBS), 16 patients with progressive supranuclear palsy (PSP), 33 patients with idiopathic Parkinson's disease, and 24 healthy controls. We compared spectral power as well as amplitude and frequency of power peaks between groups.ResultsAtypical parkinsonism was associated with spectral slowing, distinguishing both CBS and PSP from Parkinson's disease (PD) and age‐matched healthy controls. Patients with atypical parkinsonism showed a shift in β peaks (13–30 Hz) toward lower frequencies in frontal areas bilaterally. A concomitant increase in θ/α power relative to controls was observed in both APS and PD.ConclusionSpectral slowing occurs in atypical parkinsonism, affecting frontal β oscillations in particular. Spectral slowing with a different topography has previously been observed in other neurodegenerative disorders, such as Alzheimer's disease, suggesting that spectral slowing might be an electrophysiological marker of neurodegeneration. As such, it might support differential diagnosis of parkinsonian syndromes in the future. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
Extensor carpi radialis brevis motor branch transfer to the deep branch of the ulnar nerve to repair intrinsic hand palsy in neonatal lower brachial plexus and spinal cord injury: A case report
Authors: Soldado Francisco , Nguyen Trong‐Quynh , Gonzalez‐Morgado Diego , Rojas‐Neira Juliana , Nguyen Dinh‐Hung , Diaz‐Gallardo Paula 
Published: May 2023

AbstractConcomitant lower neonatal brachial plexus palsy (Klumpke) and spinal cord injury is extremely rare but with a clearly established mechanism of injury pattern. No successful surgical techniques have been reported to date to restore intrinsic hand function. We report a case of successful transfer of the extensor carpi radialis brevis motor branch to the deep branch of the ulnar nerve to repair intrinsic hand palsy. Three‐month‐old boy with the diagnosis of left Klumpke paralysis and thoracic spinal cord injury associating left Horner's sign, intrinsic minus deformity of all the digits, and thenar muscle paralysis in the upper limb. Both lower limbs were fully paralyzed. Cervical magnetic resonance imaging (MRI) revealed spinal cord narrowing from T1 to T5 and pseudo‐meningoceles involving the left C8 through T3 roots. Since no spontaneous recovery was apparent by 6.5 months and surgical exploration showed pronator quadratus denervation, the ECRB motor branch deep branch was transferred to the ulnar nerve (DBUN) with interposed a 7.5 cm‐long sural nerve graft. By 18 months post‐operatively, all the digits showed complete active IP extension. Thirty‐six months after surgery, no signs of first dorsal interosseous nerve or thenar muscle reinnervation were present, thus an extensor carpi ulnaris opponensplasty was performed. ECRB motor branch might be a valuable tool to restore finger intrinsic function in these uncommon cases.
Noninvasive brain stimulation in the treatment of functional urological and pelvic floor disorders: A scoping review
Authors: Mazeaud Charles , Salazar Betsy H. , Khavari Rose 
Published: May 2023

AbstractIntroductionFunctional pelvic floor disorders (PFD) such as bowel and bladder dysfunctions can be challenging to manage with our current therapeutic modalities. Recently, noninvasive brain stimulation has emerged as a novel strategy for noninvasive pelvic floor management. Here, we assessed the current state of research on this topic.MethodsA scoping review was conducted with Pubmed, Web of Science, and Embase, in conjunction with clinicaltrials.gov, encompassing all manuscripts published without past time limit up until June 30, 2022.ResultsOf the 880 abstracts identified in a blind selection by two reviewers, 14 publications with an evidence level of 1 or 2 (Oxford scale) were eligible and included in this review. Review articles, case reports (<5 patients), letters, and protocol studies were excluded. PFDs were described as either pelvic pain or lower urinary tracts symptoms (LUTS) with repeated transcranial magnetic stimulation (rTMS) as the most common treatment modality. Despite heterogeneous therapeutic protocols, significant improvements were observed such as reduction in postvoid residual of urine, increased bladder capacity, improved voiding flow paraments, and decreased chronic pelvic, and bladder pain. No appreciable adverse effects were noted. However, low sample populations allowed only provisional conclusions.ConclusionNoninvasive transcranial neurostimulation for LUTS and pelvic pain is emerging as an effective tool for clinicians to utilize in the future. Further investigation is needed to elucidate the full significance of the indicated outcomes.