bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decellularized Liver Nanofibers Enhance and Stabilize the Long‐Term Functions of Primary Human Hepatocytes In Vitro
Authors: Liu Jennifer S. , Madruga Liszt Y. C. , Yuan Yang , Kipper Matt J. , Khetani Salman R. 
Published: March 2023

AbstractOwing to significant differences across species in liver functions, in vitro human liver models are used for screening the metabolism and toxicity of compounds, modeling diseases, and cell‐based therapies. However, the extracellular matrix (ECM) scaffold used for such models often does not mimic either the complex composition or the nanofibrous topography of native liver ECM. Thus, here novel methods are developed to electrospin decellularized porcine liver ECM (PLECM) and collagen I into nano‐ and microfibers (≈200–1000 nm) without synthetic polymer blends. Primary human hepatocytes (PHHs) on nanofibers in monoculture or in coculture with nonparenchymal cells (3T3‐J2 embryonic fibroblasts or primary human liver endothelial cells) display higher albumin secretion, urea synthesis, and cytochrome‐P450 1A2, 2A6, 2C9, and 3A4 enzyme activities than on conventionally adsorbed ECM controls. PHH functions are highest on the collagen/PLECM blended nanofibers (up to 34‐fold higher CYP3A4 activity relative to adsorbed ECM) for nearly 7 weeks in the presence of the fibroblasts. In conclusion, it is shown for the first time that ECM composition and topography synergize to enhance and stabilize PHH functions for several weeks in vitro. The nanofiber platform can prove useful for the above applications and to elucidate cell‐ECM interactions in the human liver.
A Liquid Triboelectric Series
Authors: Yoo Donghyeon , Jang Sunmin , Cho Sumin , Choi Dongwhi , Kim Dong Sung 
Published: March 2023

AbstractThe triboelectric series is a generally accepted method for describing the triboelectric effect. It provides a way to control the double face of the ubiquitous triboelectric effect: causes of unpredictable accidents and the resultant surface charge as energy sources. However, previous studies have been biased in solids despite being observed in liquids (liquid‐solid contact electrification). Therefore, a liquid triboelectric series is necessary to be established to manipulate the liquid triboelectric effect according to the appropriate goal. In this study, a liquid triboelectric series is first established to describe the triboelectric properties of each liquid when contact electrification occurs with a solid surface. The liquid triboelectric series covers electrolytes, organic solvents, oxidants, and higher sugar alcohols. Common chemical groups can be derived from the liquid triboelectric series that hydroxyl groups enhance, and benzene groups suppress the liquid triboelectric effect. The results are demonstrated by the amplified efficiency of an energy harvester and particle contamination after surface washing. This study will play a pivotal role in understanding the liquid‐solid contact electrification phenomenon and providing new perspectives on the applications of the liquid triboelectric effect.
Go‐along interview assessment of community health priorities for neighborhood renewal
Authors: Berg Kristen A. , DeRenzo Maria , Carpiano Richard M. , Lowenstein Irwin , Perzynski Adam T. 
Published: March 2023

AbstractHealthcare systems are increasingly investing in approaches to address social determinants of health and health disparities. Such initiatives dovetail with certain approaches to neighborhood development, such as the EcoDistrict standard for community development, that prioritize both ecologically and socially sustainable neighborhoods. However, healthcare system and community development initiatives can be untethered from the preferences and lived realities of residents in the very neighborhoods upon which they focus. Utilizing the go‐along approach to collecting qualitative data in situ, we interviewed 19 adults to delineate residents' community health perspectives and priorities. Findings reveal health priorities distinct from clinical outcomes, with residents emphasizing social connectedness, competing intra‐ and interneighborhood perceptions that potentially thwart social connectedness, and a neighborhood emplacement of agency, dignity, and self‐worth. Priorities of healthcare systems and community members alike must be accounted for to optimize efforts that promote health and social well‐being by being valid and meaningful to the community of focus.
Computational Study on Ni−Al Bimetal‐Catalyzed Twofold C−H Annulation Reaction: Mechanism, Origin of Selectivity, and Role of SPO Ligand
Authors: Liu Guixian , Wang Ling‐Ling , Zhou Yongzhu , Zhou Jianguo , Zhang Lei 
Published: March 2023

AbstractDensity functional theory calculations have been performed to probe the reaction mechanism of the recently reported twofold C−H annulation of arylformamides with alkynes by Ni−Al bimetallic catalysis. The main pathway consisted of the formyl C−H activation, phenyl C−H activation, migratory insertion, and reductive elimination steps in succession. The desired annulation product was found to be the equilibrium‐controlled product, while the minor hydrocarbamoylation product was the kinetics‐controlled product. Calculated free‐energy profiles indicated that the annulation and hydrocarbamoylation pathways could intersect with each other and merge into a mechanism unity, in which the reaction system entered the hydrocarbamoylation pathway in early stage and switched to the annulation pathway afterwards via suitable transition states. The modified mechanism picture demonstrated the phenyl C−H activation step to be the rate‐determining step with a free‐energy barrier of 27.7 kcal/mol. However, this barrier was only 19.4 kcal/mol on the basis of a single annulation pathway, which could not satisfactorily agree with the experimental conditions and kinetic isotope effects. In addition, the crucial role of the bifunctional secondary phosphine oxide ligand in promoting the reactivity and selectivity was elucidated in detail.
Decoupling Redox Hopping and Catalysis in Metal‐Organic Frameworks ‐based Electrocatalytic CO2 Reduction
Authors: Li Xinlin , Surendran Rajasree Sreehari , Gude Venkatesh , Maindan Karan , Deria Pravas 
Published: March 2023

AbstractTraditional MOF e‐CRR, constructed from catalytic linkers, manifest a kinetic bottleneck during their multi‐electron activation. Decoupling catalysis and charge transport can address such issues. Here, we build two MOF/e‐CRR systems, CoPc@NU‐1000 and TPP(Co)@NU‐1000, by installing cobalt metalated phthalocyanine and tetraphenylporphyrin electrocatalysts within the redox active NU‐1000 MOF. For CoPc@NU‐1000, the e‐CRR responsive CoI/0 potential is close to that of NU‐1000 reduction compared to the TPP(Co)@NU‐1000. Efficient charge delivery, defined by a higher diffusion (Dhop=4.1×10−12 cm2 s−1) and low charge‐transport resistance (
=59.5 Ω) in CoPC@NU‐1000 led FECO=80 %. In contrast, TPP(Co)@NU‐1000 fared a poor FECO=24 % (Dhop=1.4×10−12 cm2 s−1 and
=91.4 Ω). For such a decoupling strategy, careful choice of the host framework is critical in pairing up with the underlying electrochemical properties of the catalysts to facilitate the charge delivery for its activation.
Utilizing Nitroarenes and HCHO to Directly Construct Functional N‐Heterocycles by Supported Cobalt/Amino Acid Relay Catalysis
Authors: Sun Jia‐Lu , Ci Chenggang , Jiang Huanfeng , Dixneuf Pierre H. , Zhang Min 
Published: March 2023

AbstractDue to the generation of multiple intermediates during the nitroarene reduction, precise interception of single one to develop tandem reactions involving both C−C and C−N bond formations still remains a significant challenge. Herein, the relay catalysis of a supported bifunctional cobalt catalyst with l‐proline has been successfully applied to establish a bran‐new reductive annulation reaction of nitroarenes and formaldehyde, which enables direct and diverse construction of both symmetrical and unsymmetrical 1,3‐diaryl imidazolines. It proceeds with operational simplicity, good substrate and functionality compatibility, and excellent step and atom‐efficiency. Mechanistic studies reveal that the Co‐catalyst exhibits a synergistic effect on the formation of key N‐hydroxy imine, and the l‐proline subsequently facilitates the key C−C bond formation. The current work opens a door to develop useful transformations with nitroarenes by reduction‐interrupted strategy.
A Direct Formaldehyde Fuel Cell for CO2‐Emission Free Co‐generation of Electrical Energy and Valuable Chemical/Hydrogen
Authors: Yang Yang , Wu Xuexian , Ahmad Munir , Si Fengzhan , Chen Shujing , Liu Chunhua , Zhang Yan , Wang Lei , Zhang Jiujun , Luo Jing‐Li , Fu Xian‐Zhu 
Published: March 2023

AbstractConverting carbon‐based molecular fuels into electricity efficiently and cleanly without emitting CO2 remains a challenge. Conventional fuel cells using noble metals as anode catalysts often suffer performance degradation due to CO poisoning and a host of problems associated with CO2 production. This study provides a CO2‐emission‐free direct formaldehyde fuel cell. It enables a flow of electricity while producing H2 and valuable formate. Unlike conventional carbon‐based molecules electrooxidation, formaldehyde 1‐electron oxidation is performed on the Cu anode with high selectivity, thus generating formate and H2 without undergoing CO2 pathway. In addition, the fuel cell produces 0.62 Nm3 H2 and 53 mol formate per 1 kWh of electricity generated, with an open circuit voltage of up to 1 V and a peak power density of 350 mW cm−2. This study puts forward a zero‐carbon solution for the efficient utilization of carbon‐based molecule fuels that generates electricity, hydrogen and valuable chemicals in synchronization.
Improving bioluminescence of a minimal luciferase by adding a charged oligopeptide: picALuc2.0
Authors: Ohmuro‐Matsuyama Yuki , Matsui Hayato , Kanai Masaki , Furuta Tadaomi 
Published: March 2023

AbstractLuciferases are widely used as reporter proteins in diverse fields from basic biology to medical and environmental researches. Development of luciferase applications for reporter proteins requires small size without target inhibition, appropriate genomic insertion for high expression level, and bright emission for detection sensitivity. We previously developed the minimal luciferase picALuc, but its luminescence was still dim compared to other bright luciferases in terms of expression in Escherichia coli. In this study, diverse additions of oligopeptides with charged residues (eight amino acids in total) to the C‐terminus of picALuc enhanced luminescence by up to approximately 50‐fold, that is, enhanced enzymatic activity. Moreover, these high luminescence activities were achieved in bacterial and mammalian expression, suggesting their further applicability in many expression systems. The finding in this study that the simple addition of oligopeptides with charged residues (or charge engineering of this kind) enhances enzymatic activity may be applied to a wide variety of enzymatic reactions and protein functions.
Synthesis and Antibacterial Activity Evaluation of Imidazole Derivatives Containing 6‐Methylpyridine Moiety
Authors: Xu Wen‐Bo , Meng Yu‐Qing , Sun Jingxin , Yang Yu‐Xuan , Li Wan‐Xin , Wang Man‐Yu , Piao Ming‐Guan , Li Siqi , Quan Jishan , Jin Cheng‐Hua 
Published: March 2023

AbstractA series of 2‐cyclopropyl‐5‐(5‐(6‐methylpyridin‐2‐yl)‐2‐substituted‐1H‐imidazol‐4‐yl)‐6‐phenylimidazo[2,1‐b][1,3,4]thiadiazoles (15a–t and 16a–f) were synthesized and their antibacterial activities were evaluated. More than half of the compounds showed moderate or strong antibacterial activity. Among them, compounds 15t (MIC=1–2 μg/mL) and 16d (MIC=0.5 μg/mL) showed the strongest antibacterial activities. Notably, compound 16d did not exhibit cytotoxicity in HepG2 cells and did not show hemolysis like the positive control compound Gatifloxacin. The results suggest that compound 16d should be further investigated as a candidate antibacterial agent.
Supraventricular tachycardia and deep vein thrombosis following Moderna vaccination: A case series
Authors: Bhandari Niroj , Chaudhary Aashutosh , Acharya Suyash , Acharya Shiwani Sharma , Paudel Kusum , Karmacharya Robin Man , Vaidya Satish , Kunwar Kajol , Bhatt Swechha 
Published: March 2023

AbstractThere have been reports of deep vein thrombosis and supraventricular tachycardia following the Moderna vaccination. The timing of SVT and DVT just after vaccination in our case series could suggest possible temporal relationships to the vaccination. But further studies are needed to establish such evidence.
Ethanol Dry Reforming over Bimetallic Ni‐Containing Catalysts Based on Ceria‐Zirconia for Hydrogen Production
Authors: Fedorova Valeria , Bespalko Yulia , Arapova Marina , Smal Ekaterina , Valeev Konstantin , Prosvirin Igor , Sadykov Vladislav , Parkhomenko Ksenia , Roger Anne‐Cécile , Simonov Mikhail 
Published: March 2023

AbstractMixed Ce−Zr oxides with a fluorite structure were synthesized by the solvothermal method in a supercritical medium of isopropanol. The prepared mixed oxides Ce0.5Zr0.5O2 and Ce0.75Zr0.25O2 were used as supports for 5 wt%Ni and bimetallic 2.5 wt%Ni+2.5 wt%Co catalysts. The catalysts were fully characterized (specific surface area, morphology, particle size, phase composition, surface atomic composition) and tested in ethanol dry reforming.The dependences of the conversion of C2H5OH and CO2, the yield of CO and H2, and the H2/CO ratio were estimated. The effect of the supported metals interaction and the effect of Ce/Zr ratio in the support on the catalytic activity were shown. The presence of nickel in the composition of the catalyst is necessary for high catalytic activity. It has been shown that the addition of cobalt cannot contribute to increase the catalytic activity and stability, but helps to decrease the deposition of carbon and significantly reduces the rate of carbon formation during ethanol dry reforming comparing to the monometallic nickel material.
Ru‐Catalyzed Hydrogenolysis of Methanol: A Computational and Kinetics Study
Authors: Sackers Nina M. , Nikodemus Julia , Palkovits Regina , Sautet Philippe , Hausoul Peter J. C. 
Published: March 2023

AbstractIn spite of numerous kinetic studies, the mechanism of the polyol hydrogenolysis on a Ru catalyst surface is still not fully understood. Here, the decomposition of the C1 alcohol methanol can serve as an entry to the clarification of the mechanism. Therefore, kinetic experiments using a Ru/C catalyst and periodic DFT calculations on a Ru(0001) model surface were performed. A modeling of the Ru surface revealed that the surface is most likely covered with hydrogen adsorbates under experimental conditions. On a clean Ru model surface, the overall activation barrier is lower if less dehydrogenation steps occur on the substrate methanol before the C−O bond cleavage. Transferring the results from the clean to the hydrogen‐saturated Ru surface, the pathway via CH2O O−H formation and subsequent C−O cleavage is found to be the most favorable. This study shows that the surface coverage has a significant influence on the activation barrier.
Selective Oxidation of Alkylarene in H2O Catalyzed by the Polyoxometalate Supported Chromium Catalyst
Authors: Zhao Chengchun , Xie Ya , Shi Da , Yu Han 
Published: March 2023

AbstractThe selective oxidation of alkylarene is a quite straightforward and appealing way for production of aromatic aldehydes, ketones and aromatic acid derivatives. We synthesized Cr(III) catalyst, (NH4)3[CrMo6O18(OH)6], with reasonable activity towards selective oxidation of alkylarenes under mild conditions. The catalyst is supported by a central chromium mononucleus and a cyclic inorganic ligand composed of six MoVIO6 octahedra, avoiding the disadvantages of expensive and non‐recyclable organic ligand. It is efficient, green and can be recycled at least six times with high activity. At the same time, a wide range of substituted alkylarenes were found to be compatible, providing the corresponding carbonyl compounds in moderate‐to‐high yield.
Engineered Biocatalysts for Enantioselective Reductive Aminations of Cyclic Secondary Amines
Authors: Burke Ashleigh J. , Lister Thomas M. , Marshall James R. , Brown Murray J. B. , Lloyd Richard , Green Anthony P. , Turner Nicholas J. 
Published: March 2023

AbstractReductive aminases (RedAms) have recently emerged as promising biocatalysts for the synthesis of chiral secondary amines by coupling primary amines with aldehydes/ketones. However, access to tertiary amines remains more problematic, particularly when coupling ketones with secondary amines. Here we show that the scope of these enzymes can be extended to allow selective reductive aminations of cyclic secondary amines, such as piperidines and morpholines, with both aldehydes and ketones. These biotransformations provide access to important motifs found in active pharmaceutical ingredients and other bioactive molecules. RedAm‐361, discovered from a metagenomic library, was engineered via directed evolution to allow efficient coupling of cyclic amines with carbonyl partners, including dynamic kinetic resolutions of α‐functionalized aldehydes and enantioselective amination of ketones. These RedAms now serve as valuable scaffolds for the engineering of industrial biocatalysts to produce key pharmaceutical intermediates.
Catalytic Degradation of Aliphatic Ethers using the Lewis Superacidic Bis(perfluoropinacolato)silane
Authors: Tschernuth Florian S. , Bichlmaier Lukas , Inoue Shigeyoshi 
Published: March 2023

AbstractThe exceptional catalytic activity of a neutral Si(IV) Lewis superacid was highlighted on the degradation of oligo‐ and polyethers via ring closing metathesis. Model reaction experiments with diglyme and 1,5‐dimethoxypentane outlined a significantly increased reactivity surpassing comparable Lewis acid catalyst presented in the literature. The high observed activity was explained by the kinetic effect of the bulky perfluorinated substituents, blocking a second coordination site that is responsible for catalyst deactivation by substrate chelation. Further experiments with aliphatic oligoethers outlined a gradual activity loss of the catalyst due to mechanistic disparities favoring side‐reactions when more than one internal product unit is present. The extraordinary affinity for the cleavage of etheric C−O bonds was ultimately demonstrated by the reaction with diethylether, producing a pentavalent silicate species that was fully characterized including SC‐XRD analysis.
Peroxygenase‐Promoted Enzymatic Cascades for the Valorisation of Fatty Acids
Authors: Wu Yinqi , Paul Caroline E. , Hilberath Thomas , Jongkind Ewald P. J. , Zhang Wuyuan , Alcalde Miguel , Hollmann Frank 
Published: March 2023

AbstractUtilisation of fatty acids generally relies on pre‐existing functional groups such as the carboxylate group or C=C‐double bonds. Addition of new functionalities into the hydrocarbon part opens up new possibilities for fatty acid valorisation. In this contribution we demonstrate the synthetic potential of a peroxygenase mutant AaeUPO−Fett for selective fatty acid oxyfunctionalisation. The ω‐1 hydroxy fatty acid (esters) produced are further transformed into lactones, alcohols, esters and amines via multi‐enzyme cascades thereby paving the way for new fatty acid valorisation pathways.
Searching and Evaluating Publications and Preprints Using Europe PMC
Authors: Rosonovski Summer , Levchenko Maria , Ide‐Smith Michele , Faulk Lynne , Harrison Melissa , McEntyre Johanna 
Published: March 2023

AbstractIn the field of life sciences there is a growing need for literature analysis tools that help scientists tackle information overload. Europe PubMed Central (Europe PMC), a partner of PubMed Central (PMC; National Library of Medicine, 2022), is an open access database of over 41 million life science publications and preprints, enriched with supporting data, reviews, protocols, and other relevant resources. Europe PMC is a trusted repository of choice for many life science funders (Europe PMC, 2022a), offering a suite of innovative search tools that allow users to search and evaluate the literature, including finding highly cited articles, preprints with community peer reviews, or papers referencing a proteomics dataset in the figure legend. In addition, Europe PMC utilizes text‐mining to help researchers identify key terms and find data and evidence in the literature. First‐time users often do not utilize the wealth of tools Europe PMC offers and can feel overwhelmed about how to perform the most effective search. This protocol, describing how to search and evaluate publications and preprints using Europe PMC, demonstrates how to carry out more efficient and effective literature searches using the tools provided by Europe PMC. This includes discovering the latest findings on a research topic, following research from a specific author, journal, or preprint server, exploring literature on a new method, expanding your reading list with relevant articles, as well as accessing and evaluating publications and preprints of interest. © 2023 EMBL‐EBI. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Finding articles and preprints on a topic of interestBasic Protocol 2: Accessing an articleBasic Protocol 3: Browsing the articleBasic Protocol 4: Evaluating the articleBasic Protocol 5: Refining search resultsBasic Protocol 6: Finding research by authorBasic Protocol 7: Finding a specific articleBasic Protocol 8: Finding information about a methodologyBasic Protocol 9: Finding evidence of biological interactions, relations, and modificationsBasic Protocol 10: Finding data behind a publicationBasic Protocol 11: Expanding a reading list and building a bibliographyBasic Protocol 12: Staying on top of the current literature
Smar2C2: A Simple and Efficient Protocol for the Identification of Transcription Start Sites
Authors: Murray Andrew , Vollmers Christopher , Schmitz Robert J. 
Published: March 2023

AbstractPromoters and the noncoding sequences that drive their function are fundamental aspects of genes that are critical to their regulation. The transcription preinitiation complex binds and assembles on promoters where it facilitates transcription. The transcription start site (TSS) is located downstream of the promoter sequence and is defined as the location in the genome where polymerase begins transcribing DNA into RNA. Knowing the location of TSSs is useful for annotation of genes, identification of non‐coding sequences important to gene regulation, detection of alternative TSSs, and understanding of 5′ UTR content. Several existing techniques make it possible to accurately identify TSSs, but are often difficult to perform experimentally, require large amounts of input RNA, or are unable to identify a large number of TSSs from a single sample. Many of these protocols take advantage of template switching reverse transcriptases (TSRTs), which reliably place an adaptor at the 5′ end of a first strand synthesis of cDNA. Here, we introduce a protocol that exploits TSRT activity combined with rolling circle amplification to identify TSSs with several unique advantages over existing methods. Sequence adaptors are placed on the 5′ and 3′ end of the full‐length cDNA copy of a transcript. A splint compatible with those adaptors is then used to circularize the full‐length cDNA. Linear DNA containing concatemers of the cDNA are generated using rolling circle amplification, and a sequencing library is formed by fragmenting the concatemers. This protocol is straightforward to execute, requiring limited bench time with relatively stable reagents. Using extremely low amounts of RNA input, this protocol produces large numbers of accurate, deduplicated TSSs genome wide. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Splint generationBasic Protocol 2: RNA extractionBasic Protocol 3: cDNA synthesisBasic Protocol 4: cDNA circularization and amplificationBasic Protocol 5: Library generation