bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nursing Home Geriatric Rehabilitation Care and Interprofessional Collaboration; a practice- based study
Authors: Drenth Hans , Krijnen Wim , Weerd Lourens van der , Reinders Jan Jaap , Hobbelen Hans 
Published: February 2023

Abstract
BACKGROUND Frailty and multimorbidity are common among patients in geriatric rehabilitation care (GRC). Proper care of these patients involves multiple professionals which requires optimal interprofessional collaboration to provide the best possible support. Interprofessional collaboration (IPC) goes beyond multi-professional collaboration. It requires professionals to think beyond the expertise of their own discipline and work on joint outcomes in which the patient is actively involved. This study describes the development of the GRC teams of an elderly care organization towards the IPC.
METHODS Mixed method pre-post study of 15 months. The interprofessional training program comprised team trainings, webinars, and online team sessions. Data was aggregated by administering the EPIS (interprofessional identity) and QuickScan (QS) (aspects of collaboration) measurements to GRC staff and by observations of the multi-professional team consultation (MPC) meetings of six GRC teams of an organization for elderly care in the Netherlands. ADL independence (Barthel Index) and number of inpatient days were analyzed before and after the project.
RESULTS Pretest response was 106; posttest was 84. The EPIS shows improvement on "interprofessional belonging" (P=.001, 95%CI: .566 - 2.208), "interprofessional commitment" (P=.027, 95%CI: .117 – 1.899), and overall “interprofessional identity” (P=.013, 95%CI: .622 - 5.197). On the QS, all domains improved; "shared values" (P= .009, 95%CI: .067 - .474), "context" (P= .005, 95%CI: .077 - .435), "structure & organization" (P= .001, 95%CI: .141 - .560), “group dynamics & interaction” (P<.001, 95%CI: .179 - .576), and “entrepreneurship & management” (P= .039, 95%CI: .013 - .479). A qualitative analysis of the reflection responses and MPC observations indicate a shift from multi-professional to more IPC. The mean number of inpatient days was reduced by 11.8 (P<.001, 95%CI: -17.335 - - 6.305) days.
CONCLUSIONS Within the GRC teams, there was a shift observed to more IPC and better representation of the patient's wishes and needs. ADL independence did not change, yet there was a beneficial impact on the number of inpatient days. The basis for IPC was well established, however, it remains necessary that the teams continue to develop and invest in the collaboration with each other and the patient to further improve it.
A multidimensional analysis of well-being inequality in Italy, before and after the Great Recession
Authors: Bechini Tommaso , De Muro Pasquale 
Published: February 2023

Abstract
This paper presents an analysis of well-being inequality in Italy before and after the Great Recession of 2007 / 2008, utilising multidimen- sional measures that are an extension of the Atkinson index [1], as well as gender and regional decompositions, and a comparison with traditional unidimensional monetary measures. In the selection of well- being dimensions and indicators to be included, we adopted, under the constraint of available data (the household survey IT-SILC), both the capability approach [61] and the “Constitutional Approach” recently pro- posed by [13], that allows to select dimensions which enjoy broad and enduring political consensus in the country. For the aggregation of indi- cators within and between dimensions, we adopted a non-compensatory CES-type function and then studied the resulting vector using the Multidimensional Well-being Inequality Index (MWII), a multidimen- sional version of the Atkinson by [37] and [73] that uses the weights derived from the Italian Constitution. The main findings are: there has been a rise in inequality between 2005 and 2014, income inequal- ity has worsened less than non-monetary inequality, Southern Italy has both lower levels of well-being and much higher levels of inequality for any value of the parameter, thus confirming the historical Italian regional divide as well as the absence of convergence; 4) inequality between females is higher than between males for both 2005 and 2014.
Spray-Drying Optimization for Dunaliella salina and Porphyridium cruentum Biomass
Authors: Durmaz Yaşar , Konar Nevzat , Gurbuz Basak , Polat Derya Genc , Mert Behic 
Published: February 2023

Abstract
The compositions and cultivation advantages of microalgae increase their importance as sustainable food and feed sources. However, drying and encapsulation processes are needed for widespread use. In this study, Dunaliella salina and Porphyridium cruentum biomass were encapsulated by using spray-dryer (SD) and combined D-optimal methods. The independent variables were SD inlet temperature (170-190°C), maltodextrin (25-75%, w/w, in dm) and microalgae biomass (25-75%, w/w, in dm). Prior to spray drying, P. cruentum and D. salina were cultivated in a pilot scale tubular photobioreactor (PBR) and than harvested using a conical plate centrifuge. Significant models were determined for the effects of independent variables on total carotenoids, chlorophyll-a, crude protein, moisture content and encapsulation yield, water activity, average particle size, wettability, hygroscopity, L* and C* properties for both microalgae species (P<0.05). It was determined that biochemical and cell properties and composition need to developed unique spray-drying process for each alga. Also, due to the low moisture content (>6.27 g 100 g-1) and water activity (>0.277), the average particle sizes (>143.7 µm) of the samples with high stability were advantageous for various food applications and storage. However, due to the low encapsulation yield (11.1-33.1%), we recommend encapsulation and drying of P. cruentum biomass with alternative methods to spray dryer. The extracellular, as well as the cell wall and storage polysaccharides released into the culture medium of these microalgae are possible reasons for the low encapsulation yield.
Identification of Key Gene and Pathways Associated with Oxidative Stress in Keloids
Authors: Zhang Yu , Zhang Zhe , Tang Yinke , Chu Feifei , Yu Zhou , Ma Xianjie 
Published: February 2023

Abstract
Introduction: The keloids are fibroproliferative disorder and characterized by abnormal proliferation of fibroblasts. In addition, it had been demonstrated that oxidative stress played an important role in autophagy in human fibrotic disorders. However, few researches about oxidative stress had been performed in keloids. Therefore, we performed this study to identify the oxidative stress related signatures and explore their potential mechanisms in keloids. The differential expression genes (DEGs) are obtained from GEO database, and the oxidative stress related genes (OS-genes) were obtained from GeneCards database. The crossed genes, between DEGs and OS-genes, were named as oxidative stress related differential expression genes (OS-DEGs). Then we selected the key OS-DEG and performed the enrichment analysis, interactive network analysis, immune cell infiltration, and correlation between key OS-DEG and Keloids.
Results: A total of 105 DEGs were obtained by crossing the DEGs between GSE7890 and GSE145725. And, CDK1, which was downregulated in keloids, was identified as the key OS-DEG. The functional enrichment showed that CDK1 was mainly associated with lysosome, DNA replication, and cell cycle et al. The Human Protein Atlas database delivered that CDK1 was mainly expressed in normal skin and highly expressed in fibroblasts. In addition, many immune cells, including memory resting CD4+ T cells, memory B cells, and CD8+ T cells, were obtained by immune infiltration analysis. Finally, CDK1 showed a strong association with keloids.
Conclusion: CDK1 was first stablished to play vital roles in the pathogenesis and treatment of keloids. However, the specific mechanisms of this gene in keloids have not been researched. Therefore, CDK1 provided novel research targets for further analyses in keloids.
Plasma-discharge-integrated structure for microwave power limiter
Authors: Woo Jeong Min , Ju Mun No , Lee Jae-Bok 
Published: February 2023

Abstract
A slot structure was combined with a discharge electrode to limit incident high-power microwaves via the integration of plasma discharge. At the target resonating frequency of 9.45 GHz, the surface current was concentrated at an electrode, and the electric field was enhanced by the proposed design to lower the response power level of the incident signal. When a low-power signal is injected, plasma is not generated, and the incident wave travels without insertion loss. Double-stage slot structures were utilized to broaden the band-pass characteristics in the frequency domain, and the demonstrated plasma limiter exhibited an insertion loss of 1.01 dB at 9.45 GHz. The xenon gas pressure was optimized with the shortest distance of 100 µm between the upper and lower electrodes to reduce the discharge power of the plasma. In the case of a high-power signal input, as xenon-gas breakdown occurred, the transmitted signal was close to zero, and most of the high-power signal was reflected with a blocking efficiency of 40.55 dB.
Phenome-wide investigation of the causal associations of cheese intake with obesity and obesity traits: a two-sample mendelian randomization analyses
Authors: HUANG Yu-qing , HUANG Ze , HE Guo , FENG Ying 
Published: February 2023

Abstract
Background Cheese intake has been observed to be associated with obesity, but whether this relationship was causal was still unclear. To evaluate whether cheese intake have an causality effect on obesity and related traits. Methods We performed a two-sample Mendelian randomization (MR) analysis. Summary statistics from published Genome-wide association study (GWAS) associated with obesity and related traits including body mass index (BMI), waist circumference (WC), hip circumference (HC), waist-to-hip ratio (WHR) were obtained from FinnGen Biobank, IEU OpenGWAS database and UK Biobank. The cheese intake summary dataset was obtained from a UK Biobank database including 451,486 participants. Effect estimates were evaluated using inverse-variance weighted (IVW) method. Other MR analyses, including MR Egger (bootstrap), weighted median and weighted mode methods were performed in sensitivity analyses. Results In the MR analysis, genetically predicted cheese intake were associated with a 60% decreased risk of obesity (95% CI: 0.25, 0.63, P = 9.76E-5), 72% of obesity class 1 (95% CI: 0.16, 0.51, P = 1.75E-5), 74% of obesity class 2 (95% CI: 0.13, 0.55, P = 4.12E-4), 79% of obesity class 3 (95% CI: 0.07, 0.64, P = 6.25E-3) and 52% of overweight (95% CI: 0.33, 0.71, P = 2.39E-4) per 1-standard deviation (SD) increment of cheese intake by IVW. The results also provided strong evidence that cheese intake was causally and negatively associated with BMI (β=-0.60, 95% CI: -0.90, -0.29; P = 0.000), WC (β=-0.28, 95% CI: -0.40, -0.17; P = 2.13E-6), HC (β=-0.27, 95% CI: -0.41, -0.13; P = 2.13E-4) and WHR (β=-0.39, 95% CI: -0.50, -0.27; P = 5.92E-11). Results were robust using other MR methods in sensitivity analyses. Conclusions This two-sample MR analysis found that genetically predicted cheese intake had causal effect on an decreased risk of obesity and related traits. The improvement of dietary behavior may be beneficial to prevent obesity.
Fine mapping of a Fusarium crown rot resistant locus on chromosome arm 6HL in barley by exploiting near isogenic lines, transcriptome profiling and a large near isogenic line-derived population
Authors: Gao Shang , Jiang Yunfeng , Zhou Hong , Liu Yaxi , Li Huihui , Liu Chunji , Zheng Zhi 
Published: February 2023

Abstract
Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is a chronic and serious disease affecting semi-arid regions globally. The increasing prevalence of this disease in recent years is attributed to the widespread adoption of minimum tillage and stubble retention practices. In the study reported here, we generated eight pairs of near isogenic lines (NILs) targeting a putative QTL (Qcrs.caf-6H) conferring FCR resistance in barley. Assessing the NILs confirmed the large effect of this locus. Aimed to develop markers that can be reliably used in incorporating this resistant locus into breeding programs and identify candidate genes, transcriptomic analyses were conducted against three of the NIL pairs and a large NIL-derived population consisting of 1,085 F7 recombinant inbred lines (RILs) was generated. By analysing the transcriptomic data and the fine mapping population, Qcrs.caf-6Hwas delineated into an interval of 0.9 cM covering a physical distance of ~547 kb. Six markers co-segregating with this locus were developed. Based on differential gene expression and SNP variations between the two isolines among the three NIL pairs, candidate genes underlying the resistance at this locus were detected. These results would improve the efficiency of incorporating the targeted locus into barley breeding programs and facilitate the cloning of causal gene(s) responsible for the resistance.
Platinum-Tin Anode for Direct Sugarcane Juice Fuel Cell, a Viable Alternative for Bioenergy Systems: Clean Energy without Production of Vinasse
Authors: Villardi Bruno D.Q. , Maia Victoria A. , Nandenha Julio , Zambiazi Priscilla J. , Souza Rodrigo F.B. , Neto Almir O. 
Published: February 2023

Abstract
In this study, a bioenergy system utilizing sugarcane juice as fuel was developed using PtSn/C as an anode in various atomic compositions. Results showed that the Pt90Sn10/C composition exhibited a high maximum power density 8.7 mW cm− 2 and open circuit potential of 0.75 V. This research suggests that PtSn/C is a promising material for power devices due to its electronic and bifunctional properties. Analysis of XRD results showed that the lattice parameters expanded, indicating the insertion of Sn into Pt. TEM images and histograms also revealed an increase in average particle size and the presence of tin oxide on the material surface, indicating the effect of tin enrichment in the composition. Overall, this study presents a viable alternative for bioenergy systems that eliminate the production of vinasse.
Microaglae-based wastewater treatment for circular economy: Efficiency of indigenous green microalgae for nutrient removal and resource recovery
Authors: Umetani Ikumi , Sposób Michał , Tiron Olga 
Published: February 2023

Abstract
Microalgae biotechnology can strengthen circular economy
concepts in the wastewater treatment sector. This study
demonstrated the potential of the Norwegian microalgae strains of
Tetradesmus wisconsinensis, Lobochlamys segnis,
and Klebsormidium flaccidum for wastewater nutrient
removal and valuable biomass generation. In the laboratory
batch-experiment with synthetic municipal wastewater, all strains
accomplished total removal of nitrogen and phosphorus. L.
segnis removed all NH4+ and
PO43- (initial concentration of 28 and 15 mg
L–1, respectively) earliest among others (within 4 days)
and the harvested biomass had the highest total fatty acid (TFA)
content (193 ± 12 mg g-1 dry cells). T.
wisconsinensis biomass on the other hand was superior in total
carbohydrates content (40%) while K. flaccidum produced a
protein-rich (53%) cells and accumulated fatty acids that consisted
largely of polyunsaturated fatty acids (82% of TFA). Observed
variations in biomass components can be used for a strategic
production of targeted compound in resource recovery scenarios for
the studied strains.
Integrated analyses reveal a hinge glycan regulates coronavirus spike tilting and virus infectivity
Authors: Chmielewski David , Wilson Eric , Pintilie Grigore , Zhao Peng , Chen Muyuan , Schmid Michael , Simmons Graham , Wells Lance , Jin Jing , Singharoy Abhishek , Chiu Wah 
Published: February 2023

Abstract
Coronavirus spike glycoproteins presented on the virion surface mediate receptor binding, and membrane fusion during virus entry and constitute the primary target for vaccine and drug development. How the structure dynamics of the full-length spikes incorporated in viral lipid envelope correlates with the virus infectivity remains poorly understood. Here we present structures and distributions of native spike conformations on vitrified human coronavirus NL63 (HCoV-NL63) virions without chemical fixation by cryogenic electron tomography (cryoET) and subtomogram averaging, along with site-specific glycan composition and occupancy determined by mass spectroscopy. The higher oligomannose glycan shield on HCoV-NL63 spikes than on SARS-CoV-2 spikes correlates with stronger immune evasion of HCoV-NL63. Incorporation of cryoET-derived native spike conformations into all-atom molecular dynamic simulations elucidate the conformational landscape of the glycosylated, full-length spike that reveals a novel role of stalk glycans in modulating spike bending. We show that glycosylation at N1242 at the upper portion of the stalk is responsible for the extensive orientational freedom of the spike crown. Subsequent infectivity assays support the hypothesis that this glycan-dependent motion impacts virus entry. Our results suggest a potential therapeutic target site for HCoV-NL63.
Clinical characterization and immunosuppressive regulation of IL15RA in Kidney renal papillary cell carcinoma through 324 samples
Authors: Liu Ziwei , Zou Wenda , Xu Yong , Wu Peng 
Published: February 2023

Abstract
Stimulation of renal cell carcinoma with exogenous IL-15Rα, encoded by IL15RA, could induce epithelial-mesenchymal transition (EMT) and promote cell migration and invasion. To assess the validity of IL15RA in predicting the prognosis of kidney renal papillary cell carcinoma (KIRP) and to explore its therapeutic potential for KIRP, we performed a retrospective RNA-seq data analysis of a cohort of 290 patients with KIRP from the TCGA database and 34 patients from the GEO database. We found that IL15RA is downregulated in KIRP samples compared to normal samples, but upregulated in KIRP samples with relatively higher malignancy and later stages. Univariate cox analysis and multivariate cox regression analysis suggested that upregulated IL15RA is an independent risk factor for poor prognosis of KIRP. Functional enrichment analysis showed that IL15RA in KIRP may play an essential role in inflammatory and immune response through rap1 signaling pathway. Immune cell infiltration analysis revealed that the infiltration level of M2 macrophages was positively correlated with IL15RA expression. Moreover, IL15RA showed a strong positive relationship with known inhibitory immune checkpoints, which may represent a novel mechanism of immune escape and provide new insights into the potential of immunotherapy for KIRP.
The rocky road of 55 years of change in the relationship of cardiovascular risk factors to cognition
Authors: Meirelles Osorio , Arnette Anthony , Gudnason Vilmundur , Launer L. 
Published: February 2023

Abstract
The mixed evidence that high levels of cardiovascular risk factors (CVRF) are associated with lower cognitive test scores of may be due to confounding of age across studies. We pooled and harmonized individual-level data (30,967 persons, age range 42-96y) from five prospective cohorts to examine the trajectories of betas estimating 1-year-age associations of a cognitive outcome (Digit Symbol Substitution Test; DSST) to five CVRF: systolic and e blood pressure, total cholesterol, fasting glucose and body mass index. Linear and quadratic piecewise regression models were fit to the trajectory patterns of these betas. The trajectories showed with each 1-year age increment, higher CVRF were associated with lower DSST, but associations attenuated toward zero as age increased. In addition, the pattern across age of each CVRF-DSST trajectory ranged from linear to non-liner. Without accounting for participant age in cohort comparisons, conclusions about the potential benefit on cognitive function of modifiable CVRF control will continue to be mixed and lead to delays in developing prevention programs.
Conceptual Similarity Promotes Memory Generalization At the Cost of Detailed Recollection
Authors: Melega Greta , Sheldon Signy 
Published: February 2023

Abstract
A cardinal feature of episodic memory is the ability to generalize knowledge across similar experiences to make inference about novel events. Here, we tested if this ability to apply generalized knowledge exists for experiences that are similar in terms of underlying concepts, prior knowledge, and if this comes at the expense of another feature of episodic memory: forming detailed recollection of events Over three experiments, healthy participants performed a modified version of the acquired equivalence test in which they learned overlapping object-scenes associations (A-X, B-X and A-Y) and then generalized the acquired knowledge to indirectly learned associations (B-Y) and novel objects (C-X and C-Y) that were from the same conceptual category (e.g. A - pencil; B - scissors) and different categories (e.g. A - watch; B - fork). In a subsequent recognition memory task, participants made old/new judgements to old (targets), similar (lures) and novel items. Across all experiments, we found that indirect associations that were rooted in conceptual similarity knowledge led to higher rates of generalisation but reduced detailed object memory. Our findings suggest that activating prior conceptual knowledge emphasizes the generalization function of episodic memory at the expense of detailed recollection. We discuss how this trade-off between generalization and recollection functions of episodic memory result from engaging different representations during learning.
Efficient production of salvianic acid A from L-dihydroxyphenylalanine through a tri-enzyme cascade
Authors: Yang Jiahui , Wei Wanqing , Gao Changzheng , Song Wei , Gao Cong , Chen Xiulai , Liu Jia , Guo Liang , Liu Liming , Wu Jing 
Published: February 2023

Abstract
Salvianic acid A (SAA), used for treating cardiovascular and cerebrovascular diseases, possesses several pharmacological properties. However, the current methods for the enzymatic synthesis of SAA show low efficiency. Here, we constructed a three-enzyme cascade pathway in Escherichia coli BL21(DE3) to produce SAA from L-dihydroxyphenylalanine (L-DOPA). The phenylpyruvate reductase (LaPPR) from Lactobacillus sp. CGMCC 9967 is a rate-limiting enzyme in this process. Therefore, we employed a mechanism-guided protein engineering strategy to shorten the transfer distances of protons and hydrides, generating an optimal LaPPR mutant, LaPPRMu2 (H89M/H143D/P256C), with a 2.8-fold increase in specific activity and 9.3-time increase in kcat/Km value compared to that of the wild type. Introduction of the mutant LaPPRMu2 into the cascade pathway and the optimization of enzyme levels and transformation conditions allowed the obtainment of the highest SAA titer (82.55 g L− 1) ever reported in vivo, good conversion rate (91.3%), excellent ee value (99%) and the highest productivity (6.88 g L− 1 h− 1) from 90 g L− 1 L-DOPA in 12 h. This successful strategy provides a potential new method for the industrial production of SAA.