bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic signatures of feline chronic gingivostomatitis are influenced by upregulated IL6
Authors: Peralta Santiago , Grenier Jennifer K. , Webb Suzin M. , Miller Andrew D. , Miranda Ileana C. , Parker John S. L. 
Published: August 2023

AbstractFeline chronic gingivostomatitis (FCGS) is a relatively common and debilitating disease characterized by bilateral inflammation and ulceration of the caudal oral mucosa, alveolar and buccal mucosa, and varying degrees of periodontal disease. The etiopathogenesis of FCGS remains unresolved. In this study, we performed bulk RNA-seq molecular profiling of affected tissues derived from a cohort of client-owned cats with FCGS compared to tissues from unaffected animals, to identify candidate genes and pathways that can help guide future exploration of novel clinical solutions. We complemented transcriptomic findings with immunohistochemistry and in situ hybridization assays to better understand the biological significance of the results and performed RNA-seq validation of biologically relevant differentially expressed genes using qPCR assays to demonstrate technical reproducibility. Transcriptomic profiles of oral mucosal tissues in cats with FCGS are enriched with immune- and inflammation-related genes and pathways that appear to be largely influenced by IL6, and include NFKB, JAK/STAT, IL-17 and IFN type I and II signaling, offering new opportunities to develop novel clinical applications based on a more rational understanding of the disease.
Whistle repertoire and structure reflect ecotype distinction of pantropical spotted dolphins in the Eastern Tropical Pacific
Authors: Rege-Colt Manali , Oswald Julie N. , De Weerdt Joelle , Palacios-Alfaro Jose David , Austin Maia , Gagne Emma , Morán Villatoro Jacqueline Maythé , Sahley Catherine Teresa , Alvarado-Guerra Gilma , May-Collado Laura J. 
Published: August 2023

AbstractThe pantropical spotted dolphin in the Eastern Tropical Pacific (ETP) is found in two genetically and phenotypically diverged ecotypes, coastal and offshore. These habitats have distinct acoustic characteristics, which can lead to the evolution of distinct acoustic communication. Whistles are sounds widely used by dolphins to mediate species and individual recognition and social interactions. Here, we study the whistle acoustic structure and repertoire diversity of offshore and coastal pantropical spotted dolphins. Our results show that there is significantly more within- and across-group variation in whistle fundamental frequency between ecotypes than between offshore groups and between coastal groups. A Random Forest classification analysis performed with an accuracy of 83.99% and identified duration, peak and minimum frequency as the most informative variables for distinguishing between ecotypes. Overall, coastal spotted dolphins produced significantly shorter whistles that were significantly lower in frequency (peak, minimum and maximum, and start and end) than offshore dolphins. Ecotypes produced whistle repertoires that were similar in diversity, but different in contour composition, with the coastal ecotype producing more upsweep whistles than offshore dolphins. The results of this study suggest that acoustic adaptations to coastal and offshore environments could be important contributors to intraspecific variation of dolphin whistle repertoires.
Tuning the surface functionality of polyethylene glycol-modified graphene oxide/chitosan composite for efficient removal of dye
Authors: Pervez Md. Nahid , Jahid Md Anwar , Mishu Mst. Monira Rahman , Talukder Md Eman , Buonerba Antonio , Jiang Tao , Liang Yanna , Tang Shuai , Zhao Yaping , Dotto Guilherme L. , Cai Yingjie , Naddeo Vincenzo 
Published: August 2023

AbstractThere has been a lot of attention on water pollution by dyes in recent years because of their serious toxicological implications on human health and the environment. Therefore, the current study presented a novel polyethylene glycol-functionalized graphene oxide/chitosan composite (PEG-GO/CS) to remove dyes from aqueous solutions. Several characterization techniques, such as SEM, TEM, FTIR, TGA/DTG, XRD, and XPS, were employed to correlate the structure–property relationship between the adsorption performance and PEG-GO/CS composites. Taguchi’s (L25) approach was used to optimize the batch adsorption process variables [pH, contact time, adsorbent dose, and initial concentration of methyl orange (MO)] for maximal adsorption capacity. pH = 2, contact time = 90 min, adsorbent dose = 10 mg/10 mL, and MO initial concentration = 200 mg/L were found to be optimal. The material has a maximum adsorption capacity of 271 mg/g for MO at room temperature. With the greatest R2 = 0.8930 values, the Langmuir isotherm model was shown to be the most appropriate. Compared to the pseudo-first-order model (R2 = 0.9685), the pseudo-second-order model (R2 = 0.9707) better fits the kinetic data. Electrostatic interactions were the dominant mechanism underlying MO sorption onto the PEG/GO-CS composite. The as-synthesized composite was reusable for up to three adsorption cycles. Thus, the PEG/GO-CS composite fabricated through a simple procedure may remove MO and other similar organic dyes in real contaminated water.
miR-378 influences muscle satellite cells and enhances adipogenic potential of fibro-adipogenic progenitors but does not affect muscle regeneration in the glycerol-induced injury model
Authors: Mucha Olga , Podkalicka Paulina , Żukowska Monika , Pośpiech Ewelina , Dulak Józef , Łoboda Agnieszka 
Published: August 2023

AbstractSkeletal muscle regeneration relies on the reciprocal interaction between many types of cells. Regenerative capacity may be altered in different disorders. In our study, we investigated whether the deletion of miR-378a (miR-378) affects muscle regeneration. We subjected 6-week-old wild-type (WT) and miR-378 knockout (miR-378–/–) animals to the glycerol-induced muscle injury and performed analyses in various time-points. In miR-378–/– animals, an elevated abundance of muscle satellite cells (mSCs) on day 3 was found. Furthermore, fibro-adipogenic progenitors (FAPs) isolated from the muscle of miR-378–/– mice exhibited enhanced adipogenic potential. At the same time, lack of miR-378 did not affect inflammation, fibrosis, adipose tissue deposition, centrally nucleated fiber count, muscle fiber size, FAP abundance, and muscle contractility at any time point analyzed. To conclude, our study revealed that miR-378 deletion influences the abundance of mSCs and the adipogenic potential of FAPs, but does not affect overall regeneration upon acute, glycerol-induced muscle injury.
Effects of febuxostat on markers of endothelial dysfunction and renal progression in patients with chronic kidney disease
Authors: Nata Naowanit , Ninwisut Nanthawut , Inkong Pitchamon , Supasyndh Ouppatham , Satirapoj Bancha 
Published: August 2023

AbstractHyperuricemia relates to chronic kidney disease (CKD) progression and impaired endothelial function. Febuxostat is potent and effective for decreasing serum uric acid levels. Information for the effect of febuxostat treatment on markers of endothelial dysfunction and renal injury among patients with CKD remains limited. A total of 84 patients with CKD stages III-IV with asymptomatic hyperuricemia were randomly assigned to either the febuxostat (40 mg/day, N = 42) or the matching control (N = 42) group for 8 weeks. Serum asymmetric dimethylarginine (ADMA), estimated glomerular filtration rate (eGFR), urine albumin, high sensitivity C-reactive protein (hs-CRP), ankle brachial index (ABI) and serum uric acid were measured at baseline and at the end of study. Febuxostat administration significantly reduced the serum uric acid concentration among patients with CKD when compared with control [− 3.40 (95% CI − 4.19 to − 2.62) vs. − 0.35 (95% CI − 0.76 to 0.06) mg/dL; P < 0.001, respectively). No significant difference in the changes in serum ADMA, hs-CRP, eGFR and albuminuria was identified between the two groups. Subgroup analysis among patients with decreased serum uric acid after febuxostat, the estimated GFR change between the febuxostat and the control group showed significant difference at 8 weeks (2.01 (95% CI 0.31 to 3.7) vs. 0.04 (95% CI − 1.52 to 1.61) mL/min/1.73 m2; P = 0.030, respectively). Adverse events specific to febuxostat were not observed. Febuxostat effectively reduced serum uric acid in the CKD population without improving endothelial dysfunction. It was able to preserve renal function in the subgroup of patients with CKD and lower serum uric acid level after treatment.Trial registration: Thai Clinical Trials, TCTR20210224005: 24/022021 http://www.thaiclinicaltrials.org/show/TCTR20210224005.
Cost effectiveness review of text messaging, smartphone application, and website interventions targeting T2DM or hypertension
Authors: Willems Ruben , Annemans Lieven , Siopis George , Moschonis George , Vedanthan Rajesh , Jung Jenny , Kwasnicka Dominika , Oldenburg Brian , d’Antonio Claudia , Girolami Sandro , Agapidaki Eirini , Manios Yannis , Verhaeghe Nick ,   DigiCare 4You, Usheva Natalya , Iotova Violeta , Triantafyllidis Andreas , Votis Konstantinos , Toti Florian , Makrilakis Konstantinos , Seghieri Chiara , Moreno Luis , Dupont Sabine , Lewis Leo , Djokic Djordje , Skouteris Helen 
Published: August 2023

AbstractDigital health interventions have been shown to be clinically-effective for type 2 diabetes mellitus (T2DM) and hypertension prevention and treatment. This study synthesizes and compares the cost-effectiveness of text-messaging, smartphone application, and websites by searching CINAHL, Cochrane Central, Embase, Medline and PsycInfo for full economic or cost-minimisation studies of digital health interventions in adults with or at risk of T2DM and/or hypertension. Costs and health effects are synthesised narratively. Study quality appraisal using the Consensus on Health Economic Criteria (CHEC) list results in recommendations for future health economic evaluations of digital health interventions. Of 3056 records identified, 14 studies are included (7 studies applied text-messaging, 4 employed smartphone applications, and 5 used websites). Ten studies are cost-utility analyses: incremental cost-utility ratios (ICUR) vary from dominant to €75,233/quality-adjusted life year (QALY), with a median of €3840/QALY (interquartile range €16,179). One study finds no QALY difference. None of the three digital health intervention modes is associated with substantially better cost-effectiveness. Interventions are consistently cost-effective in populations with (pre)T2DM but not in populations with hypertension. Mean quality score is 63.0% (standard deviation 13.7%). Substandard application of time horizon, sensitivity analysis, and subgroup analysis next to transparency concerns (regarding competing alternatives, perspective, and costing) downgrades quality of evidence. In conclusion, smartphone application, text-messaging, and website-based interventions are cost-effective without substantial differences between the different delivery modes. Future health economic studies should increase transparency, conduct sufficient sensitivity analyses, and appraise the ICUR more critically in light of a reasoned willingness-to-pay threshold.Registration: PROSPERO (CRD42021247845).
Skin Tone Analysis for Representation in Educational Materials (STAR-ED) using machine learning
Authors: Tadesse Girmaw Abebe , Cintas Celia , Varshney Kush R. , Staar Peter , Agunwa Chinyere , Speakman Skyler , Jia Justin , Bailey Elizabeth E. , Adelekun Ademide , Lipoff Jules B. , Onyekaba Ginikanwa , Lester Jenna C. , Rotemberg Veronica , Zou James , Daneshjou Roxana 
Published: August 2023

AbstractImages depicting dark skin tones are significantly underrepresented in the educational materials used to teach primary care physicians and dermatologists to recognize skin diseases. This could contribute to disparities in skin disease diagnosis across different racial groups. Previously, domain experts have manually assessed textbooks to estimate the diversity in skin images. Manual assessment does not scale to many educational materials and introduces human errors. To automate this process, we present the Skin Tone Analysis for Representation in EDucational materials (STAR-ED) framework, which assesses skin tone representation in medical education materials using machine learning. Given a document (e.g., a textbook in .pdf), STAR-ED applies content parsing to extract text, images, and table entities in a structured format. Next, it identifies images containing skin, segments the skin-containing portions of those images, and estimates the skin tone using machine learning. STAR-ED was developed using the Fitzpatrick17k dataset. We then externally tested STAR-ED on four commonly used medical textbooks. Results show strong performance in detecting skin images (0.96 ± 0.02 AUROC and 0.90 ± 0.06 F1score) and classifying skin tones (0.87 ± 0.01 AUROC and 0.91 ± 0.00 F1score). STAR-ED quantifies the imbalanced representation of skin tones in four medical textbooks: brown and black skin tones (Fitzpatrick V-VI) images constitute only 10.5% of all skin images. We envision this technology as a tool for medical educators, publishers, and practitioners to assess skin tone diversity in their educational materials.
Bacterial bioindicators enable biological status classification along the continental Danube river
Authors: Fontaine Laurent , Pin Lorenzo , Savio Domenico , Friberg Nikolai , Kirschner Alexander K. T. , Farnleitner Andreas H. , Eiler Alexander 
Published: August 2023

AbstractDespite the importance of bacteria in aquatic ecosystems and their predictable diversity patterns across space and time, biomonitoring tools for status assessment relying on these organisms are widely lacking. This is partly due to insufficient data and models to identify reliable microbial predictors. Here, we show metabarcoding in combination with multivariate statistics and machine learning allows to identify bacterial bioindicators for existing biological status classification systems. Bacterial beta-diversity dynamics follow environmental gradients and the observed associations highlight potential bioindicators for ecological outcomes. Spatio-temporal links spanning the microbial communities along the river allow accurate prediction of downstream biological status from upstream information. Network analysis on amplicon sequence veariants identify as good indicators genera Fluviicola, Acinetobacter, Flavobacterium, and Rhodoluna, and reveal informational redundancy among taxa, which coincides with taxonomic relatedness. The redundancy among bacterial bioindicators reveals mutually exclusive taxa, which allow accurate biological status modeling using as few as 2–3 amplicon sequence variants. As such our models show that using a few bacterial amplicon sequence variants from globally distributed genera allows for biological status assessment along river systems.
Discovery of a novel bacterial class with the capacity to drive sulfur cycling and microbiome structure in a paleo-ocean analog
Authors: Vigneron Adrien , Vincent Warwick F , Lovejoy Connie 
Published: August 2023

Abstract
Uncultivated microbial taxa represent a large fraction of global microbial diversity and likely drive numerous biogeochemical transformations in natural ecosystems. Geographically isolated, polar ecosystems are complex microbial biomes and refuges of underexplored taxonomic and functional biodiversity. Combining amplicon sequencing with genome-centric metagenomic analysis of samples from one of the world’s northernmost lakes (Lake A, Ellesmere Island, Canadian High Arctic), we identified a novel bacterial taxon that dominates in the bottom layer of anoxic, sulfidic, relict sea water that was isolated from the Arctic Ocean some 3000 years ago. Based on phylogenomic comparative analyses, we propose that these bacteria represent a new Class within the poorly described Electryoneota/AABM5-125-24 candidate phylum. This novel class, for which we propose the name Tariuqbacteria, may be either a relict of ancient ocean conditions or endemic to this High Arctic system, provisionally providing a rare example of high-taxonomy level endemism. Consistent with the geochemistry of the bottom water, the genetic composition of the Candidatus Tariuqbacter genome revealed a strictly anaerobic lifestyle with the potential for sulfate and sulfur reduction, a versatile carbon metabolism and the capability to eliminate competing bacteria through methylarsenite production, suggesting an allelochemical influence on microbiome structure by this planktonic microbe.
“I Just Want People to Think I'm Normal”: An Interview Study of Young Swedish Women With Covert Stuttering
Authors: Samson Ineke , Nyberg Jill , Lindström Elisabeth , Schalling Ellika 
Published: August 2023

Purpose:Research indicates that there is a tendency for females who stutter, more often than males, to use coping strategies that involve covering their stutter, for example, by avoiding situations that require verbal participation. The aim of the study is to increase knowledge about how covert stuttering develops and its impact on self-image and quality of life for women who stutter.Method:Eleven young women who stutter covertly were interviewed, and data were subjected to qualitative content analysis. Background information was obtained from the self-report instruments measuring the impact of stuttering on different aspects of life (Overall Assessment of the Speaker's Experiences of Stuttering) and degree of perceived social anxiety (Liebowitz Social Anxiety Scale, Self-Report).Results:Three main themes were identified: (a) managing stuttering, (b) personal aspects, and (c) stuttering as a phenomenon. Shame and a desire to fit in emerged as distinct motives for covering stuttering. The women described that stuttering controlled both life choices and everyday life. Development of self-image had been strongly negatively affected, resulting in social anxiety. The women expressed a particular vulnerability of being a woman who stutters, due to societal norms of female behavior and a lack of female role models who stutter.Conclusions:The choice of coping strategy was motivated by a desire to “be normal.” As a result, stuttering had come to dominate life and affect self-image and life choices. The study highlights the importance for clinicians to be alert to and aware of the fact that the experiences of women who stutter can lead them to develop coping strategies that have far-reaching negative consequences.
Early Precursors of Reading Development in Simultaneous Bilinguals: A Systematic Review and Meta-Analysis
Authors: Bhalloo Insiya , Molnar Monika 
Published: August 2023

Purpose:
Literacy precursors are cognitive, linguistic, and oral-language skills that predict future reading skills in children as young as 4 years. Speech-language pathologists and educators utilize these precursors as assessment tools to identify children at risk for reading difficulties. Most current tools are developed based on monolinguals (predominantly in English), despite the significant percentage of bilinguals globally. As such, bilingual children are typically assessed on tools developed for monolinguals in research and clinical settings. Despite this common practice, there is a lack of comprehensive synthesis on whether these precursors are a reliable indicator of reading skills in bilingual children. Our article examines whether literacy precursors commonly used with monolinguals are associated with reading development in simultaneous bilinguals.

Method:

Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses and Cochrane guidelines, our review includes four databases (Linguistics and Language Behavior Abstracts, Educational Resources Information Center, Modern Language Association, and PsycINFO), in addition to gray-literature and manual reference-list searches. To control for age of acquisition and language dominance variability, we included typically developing simultaneous bilinguals exposed to both languages before age 3 years (
N
= 5,942). We analyzed reported statistical associations between code-related or oral-language precursors and reading outcome measures, using correlational meta-analyses.

Results:
The 41 reports, which met inclusion/exclusion criteria, were published between 1977 and 2022. The average age at assessment was 7;5 (years;months; range: 3;0–11;0), with children speaking over 21 bilingual language combinations. Our meta-analysis demonstrated significant within-language correlations and cross-language transfer effects for code-related (e.g., phonological awareness) and oral-language (e.g., vocabulary and morphological awareness) precursors. Semantic awareness, however, was not a reliable predictor in bilinguals.

Conclusions:
Phonological awareness and vocabulary measures—even if originally developed for monolingual children—can form a meaningful component of early literacy assessment in simultaneous bilingual children: These precursors may be used as assessment tools across heritage and societal languages in research and clinical practice. Future research suggestions within this domain are also discussed.

Supplemental Material:

https://doi.org/10.23641/asha.23880465
Binary masses and luminosities with Gaia DR3
Authors: Chevalier S. , Babusiaux C. , Merle T. , Arenou F. 
Published: August 2023

Context. The recent third data release (DR3) of Gaia has brought some new exciting data about stellar binaries. It provides new opportunities to fully characterize more stellar systems and contributes to enriching our global knowledge of stellar behaviour.
Aims. By combining the new Gaia non-single stars catalogue with double-lined spectroscopic binaries (SB2), we can determine the individual masses and luminosities of the components. To fit an empirical mass-luminosity relation in the Gaia G band, lower-mass stars must be added. These masses can be derived using Gaia-resolved wide binaries combined with literature data.
Methods. Using the BINARYS tool, we combined the astrometric non-single star solutions in the Gaia DR3 with SB2 data from two other catalogues: the 9th Catalogue of Spectroscopic Binary orbits (SB9), and APOGEE. We also searched for low-mass stars that are resolved in Gaia with direct imaging and HIPPARCOS data or with a literature mass fraction.
Results. The combination of Gaia astrometric non-single star solutions with double-lined spectroscopic data enabled us to characterize 43 binary systems with SB9 and 13 systems with APOGEE. We furthermore derived the masses of 6 low-mass binaries that are resolved with Gaia. We then derived an empirical mass-luminosity relation in the Gaia G band down to 0.12 ℳ⊙.