bluuebunny/crossref_metadata_2025_split huggingface dataset:
In-service training for improving the implementation of competency-based curriculum in English foreign language teaching: is planning effective?
Authors: Msamba Enitha M. , Msuya Erasmus A. , Anangisye William A.L. 
Published: May 2023

Purpose
In Tanzania, in-service training was implemented to facilitate teachers understanding and implementation of the competency-based curriculum in the English language. This study aims to evaluate whether or not the in-service training was effectively planned to achieve its goals.

Design/methodology/approach
The study used a narrative research design that focuses on analysing the story of a particular event, object or programme as it occurred in a specific social context. Thirty-two purposefully selected respondents were interviewed, and data were analysed using the thematic approach.

Findings
The study found strengths and weaknesses in the in-service training planning process. The strength included coherence between the need for in-service training and country priority. Moreover, collective participation was promoted since all trainees were English language teachers. Perceived weaknesses included a lack of coherence between training objectives and teachers’ actual needs. Equally, collective participation was limited because trainees taught mixed grades with varied work environments and experiences. Besides, heads of schools were not involved in training planning. Even those tasked with a lead role in cascading training to untrained teachers lacked the confidence and competence to do so.

Originality/value
The study contributes to the body of knowledge by uncovering weaknesses associated with in-service planning in Tanzania. The study calls upon the future planners of in-service training to consider the elements of effective in-service training during the planning and ensure that the views of all stakeholders at the macro and micro levels are assessed for quality programme implementation and better results.
Senior tourism: travel motivators and perceived constraints and risks for the elderly
Authors: Aggarwal Arun , Hajra Vandita , Kukreja Vinay 
Published: May 2023

Purpose
To cater to the senior tourist market, it is essential to comprehend the factors motivating and deterring them from international travel post-COVID-19. This study aims to focus on senior citizens’ destination choice intentions and aims to develop a model that prioritizes positive and negative factors leading to international travel destination choices. It uses push–pull factors, perceived travel risks (PTRs) and perceived travel constraints (PTCs).

Design/methodology/approach
Decision-making trial and evaluation laboratory (DEMATEL) and fuzzy technique for order of preference by similarity to ideal solution (Fuzzy TOPSIS) are two multi-criteria decision-making (MCDM) techniques used to identify connections between variables and determine their relative importance in the decision-making model.

Findings
DEMATEL found push and pull factors are “effects” while PTCs and PTRs are “causes” affecting senior citizens’ destination choices. Push factors and PTCs have a greater impact than pull factors and PTRs. Fuzzy TOPSIS highlighted “improving health and wellness” and “self-fulfillment and spirituality” as key push factors, “health safety and security quotient” as the most important pull factor, and “interpersonal constraints” as the most critical PTC. Finally, “health risks” is the top PTR.

Originality/value
This paper adds to the tourism literature by looking at the relationship between senior tourists’ motivation, PTRs and PTCs and showing how the subfactors affect their choice of destination rank. The data analysis techniques used in this study are also novel, having never been used before in senior tourism research. Finally, even though there is a lot of research on senior tourism, not much is known about how Indian senior tourists act. In light of this study’s findings, practical recommendations were offered to tourism stakeholders worldwide, interested in tapping into the market of Indian outbound senior tourists or repositioning product or destination offerings to take this promising market or similar markets into account.
Optimizing species selection for the structural timbers of traditional farmhouses in a snowy rural area of northeastern Japan
Authors: Ida Hideyuki , Sato Takuma , Rikukawa Yuta , Abe Reina , Hoyano Shigeo , Tsuchimoto Toshikazu 
Published: May 2023

AbstractVernacular houses constructed using local woods contain information about forest‐resource use. To clarify how timber was used to construct wooden farmhouses, we identified the timber species composition of 11 houses built between 1845 and about 1940 in Tadami, a snowy rural area in northeastern Japan, and interviewed 70 residents. We recorded 2004 timber parts (99–308 parts per house) and 171.2 m3(1828 parts) of known species (14 taxa).Cryptomeria japonica(cedar) andPinus parvifloravar.pentaphylla(white pine) were used for all essential structural parts and accounted for 44% and 39% of the total timber volume, respectively.Fagus crenata(beech) was the third most commonly used species (7%), for roof supports, beams, and girders. The interviews revealed that the timber was typically sourced from local private or common woodlands within 1 km and that local craftsmen selected and hauled the trees. Considering the region's unique and complex vegetation, as land‐cover distribution is strongly influenced by frequent avalanches, the timber was likely managed within local stands of tall trees, and the most available species would have been cedar (although whether native or planted is unknown), followed by native white pine on mountain ridges around settlements. In comparison, beech stands that could supply sufficient amounts of timber might have been limited. Based on the characteristics of the trees used as house timber in Tadami, we conclude that despite the surrounding harsh, snowy conditions, locally available species of tall trees were historically selected for timbers.
Contribution of genetic analyses to semi‐natural grassland biodiversity conservation in Japan
Authors: Nakahama Naoyuki , Kurata Seikan , Ushimaru Atushi 
Published: May 2023

AbstractSemi‐natural grasslands maintained by moderate anthropogenic disturbance have been rapidly declining worldwide in recent decades, resulting in the progressive degradation of biodiversity therein. Similarly, in Japan, many semi‐natural grassland organisms are threatened with extinction owing to the decline of semi‐natural grasslands, and their conservation and management has attracted considerable attention. Although ecological studies using genetic information are expected to contribute to the conservation of semi‐natural grassland organisms, no reviews have been conducted on the potential roles of these studies in grassland conservation in Japan. Here, we review the current trends in conservation‐ecological study on Japanese semi‐natural grassland organisms using genetic information by grouping into five categories: (1) monitoring of genetic diversity and spatial genetic structure, (2) exploration of the genetic consequences of management practices, (3) prevention of genetic disturbance, (4) elucidation of natural history traits, and (5) estimation of the origin and demography. Furthermore, the applicability of studies using high‐throughput sequencing, which has been rapidly progressing in recent years, is highlighted. This review provides the methods and perspectives on the use of genetic information to conserve biodiversity in semi‐natural grasslands.
Delayed elimination in humans after ingestion of colchicine: Two fatal cases of colchicine poisoning
Authors: Deng Hongxiao , Xiang Ping , Zhang Sujing , Wu Hejian , Liu Wei , Yan Hui 
Published: May 2023

AbstractColchicine has been widely used in the treatment of acute gout over the years, but it has a narrow therapeutic index, and overdose can be life threatening. A method using ultra‐high‐performance liquid chromatography–tandem mass spectrometry system was applied in two fatal cases of colchicine poisoning in this study to the determination of colchicine in blood. In case 1, a 19‐year‐old man suffered from depression and ingested 160 colchicine tablets (each 0.5 mg). The concentration of colchicine in his blood samples showed a fluctuating trend and kept above the therapeutic steady‐state concentration for 5 days. In case 2, a 70‐year‐old female patient with a history of gout and chronic colchicine intake ingested five times the usual dose of colchicine (5 mg) and died after 12 days of medical care, with 5 ng/mL of colchicine in her blood sample. Our findings suggest that the delayed elimination and accumulation in humans after colchicine overdose could keep the concentration of colchicine maintaining above the therapeutic steady‐state concentration for many days before dying, probably along with a fluctuating trend.
Evaluation of 96‐well high‐throughput DNA extraction methods for 16S rRNA gene metabarcoding
Authors: Chapuis Marie‐Pierre , Benoit Laure , Galan Maxime 
Published: May 2023

AbstractGaining meaningful insights into bacterial communities associated with animal hosts requires the provision of high‐quality nucleic acids. Although many studies have compared DNA extraction methods for samples with low bacterial biomass (e.g. water) or specific PCR inhibitors (e.g. plants), DNA extraction bias in samples without inherent technical constraint (e.g. animal samples) has received little attention. Furthermore, there is an urgent need to identify a DNA extraction methods in a high‐throughput format that decreases the cost and time for processing large numbers of samples. We here evaluated five DNA extraction protocols, using silica membrane‐based spin columns and a 96‐well microplate format and based on either mechanical or enzymatic lysis or a combination of both, using three bacterial mock communities and Illumina sequencing of the V4 region of the 16SrRNA gene. Our results showed that none of the DNA extraction methods fully eliminated bias associated with unequal lysis efficiencies. However, we identified a DNA extraction method with a lower bias for each mock community standard. Of these methods, those including an enzymatic lysis showed biases specific to some bacteria. Altogether, these results again demonstrate the importance of DNA extraction standardization to be able to compare the microbiome results of different samples. In this attempt, we advise for the use of the 96‐well DNeasy Blood and Tissue kit (Qiagen) with a zirconia bead‐beating procedure, which optimizes altogether the cost, handling time and bacteria‐specific effects associated with enzymatic lysis.
Comparative genomics reveals the emergence of copper resistance in a non‐pigmented Xanthomonas pathogen of grapevine
Authors: Rana Rekha , Jaiswal Gagandeep , Bansal Kanika , Patil Prabhu B. 
Published: May 2023

AbstractXanthomonas citri pv. viticola (Xcv) is the causal agent of bacterial canker in grapevine. The pathogen is restricted to India, where it was first reported in the 1970s, and Brazil. In the present study, we report the first complete genome sequence of Xcv LMG965, which is a reference pathotype strain. We also report genome sequences of additional isolates from India and comparative genome‐based studies of isolates from Brazil. Apart from revealing the monophyletic origin of the pathovar, we could also confirm a common frameshift mutation in a gene that is part of the Xanthomonadin pigment biosynthetic gene cluster in all the isolates. The comparative study also revealed multiple intrinsic copper resistance‐related genes in Brazilian isolates, suggesting intense selection, possibly because of heavy and indiscriminate usage of copper as an antimicrobial agent in the orchards. There is also the association of a Tn3‐like transposase in the vicinity of the copper resistance genes, indicating a potential for rapid diversification through horizontal gene transfer events. The findings, along with genomic resources, will allow for systematic genetic and functional studies of Xcv.
Fatal limbic encephalitis as paraneoplastic neurological syndrome in a patient with lung adenocarcinoma positive for antiamphiphysin antibody after durvalumab treatment
Authors: Iwanaga Yuto , Kawaguchi Takako , Yamasaki Kei , Sato Tomoki , Kubo Naoto , Morimoto Toshiki , Isoshima Yu , Sasahara Yosuke , Orihashi Takeshi , Yatera Kazuhiro 
Published: May 2023

AbstractA 69‐year‐old Japanese male with advanced lung adenocarcinoma developed neurological symptoms after chemoradiotherapy and durvalumab maintenance therapy. He was positive for serum antiamphiphysin antibody, which is rarely seen in patients with lung adenocarcinoma. Additionally, his brain magnetic resonance images showed limbic encephalitis which led to the diagnosis of classic paraneoplastic neurological syndrome (PNS). Immune checkpoint inhibitors (ICIs) activate T cells and may also activate antineuronal antibodies that cause PNS. Durvalumab, which is an ICI, may have led to antiamphiphysin antibody‐positive PNS in our patient. Treatment with systemic high‐dose methylprednisolone was unsuccessful and he died 2 months later. PNS should be considered as one of the differential diagnoses in patients with lung cancer and neurological symptoms during, or after, ICI treatment.
A review of sources of resistance to turnip yellows virus (TuYV) in Brassica species
Authors: Macleod Kyle , Greer Shannon F. , Bramham Lawrence E. , Pimenta Ricardo J. G. , Nellist Charlotte F. , Hackenburg Dieter , Teakle Graham R. , Barker Guy C. , Walsh John A. 
Published: May 2023

AbstractTurnip yellows virus (TuYV; previously known as beet western yellows virus) causes major diseases of Brassica species worldwide resulting in severe yield‐losses in arable and vegetable crops. It has also been shown to reduce the quality of vegetables, particularly cabbage where it causes tip burn. Incidences of 100% have been recorded in commercial crops of winter oilseed rape (Brassica napus) and vegetable crops (particularly Brassica oleracea) in Europe. This review summarises the known sources of resistance to TuYV in B. napus (AACC genome), Brassica rapa (AA genome) and B. oleracea (CC genome). It also proposes names for the quantitative trait loci (QTLs) responsible for the resistances, Turnip Yellows virus Resistance (TuYR), that have been mapped to at least the chromosome level in the different Brassica species. There is currently only one known source of resistance deployed commercially (TuYR1). This resistance is said to have originated in B. rapa and was introgressed into the A genome of oilseed rape via hybridisation with B. oleracea to produce allotetraploid (AACC) plants that were then backcrossed into oilseed rape. It has been utilised in the majority of known TuYV‐resistant oilseed rape varieties. This has placed significant selection pressure for resistance‐breaking mutations arising in TuYV. Further QTLs for resistance to TuYV (TuYR2‐TuYR9) have been mapped in the genomes of B. napus, B. rapa and B. oleracea and are described here. QTLs from the latter two species have been introgressed into allotetraploid plants, providing for the first time, combined resistance from both the A and the C genomes for deployment in oilseed rape. Introgression of these new resistances into commercial oilseed rape and vegetable brassicas can be accelerated using the molecular markers that have been developed. The deployment of these resistances should lessen selection pressure for resistance‐breaking isolates of TuYV and thereby prolong the effectiveness of each other and extant resistance.
Molecular and biophysical features of hippocampal “lipid rafts aging” are modified by dietary n‐3 long‐chain polyunsaturated fatty acids
Authors: Díaz Mario , Pereda de Pablo Daniel , Valdés‐Baizabal Catalina , Santos Guido , Marin Raquel 
Published: May 2023

Abstract“Lipid raft aging” in nerve cells represents an early event in the development of aging‐related neurodegenerative diseases, such as Alzheimer's disease. Lipid rafts are key elements in synaptic plasticity, and their modification with aging alters interactions and distribution of signaling molecules, such as glutamate receptors and ion channels involved in memory formation, eventually leading to cognitive decline. In the present study, we have analyzed, in vivo, the effects of dietary supplementation of n‐3 LCPUFA on the lipid structure, membrane microviscosity, domain organization, and partitioning of ionotropic and metabotropic glutamate receptors in hippocampal lipid raffs in female mice. The results revealed several lipid signatures of “lipid rafts aging” in old mice fed control diets, consisting in depletion of n‐3 LCPUFA, membrane unsaturation, along with increased levels of saturates, plasmalogens, and sterol esters, as well as altered lipid relevant indexes. These changes were paralleled by increased microviscosity and changes in the raft/non‐raft (R/NR) distribution of AMPA‐R and mGluR5. Administration of the n‐3 LCPUFA diet caused the partial reversion of fatty acid alterations found in aged mice and returned membrane microviscosity to values found in young animals. Paralleling these findings, lipid rafts accumulated mGluR5, NMDA‐R, and ASIC2, and increased their R/NR proportions, which collectively indicate changes in synaptic plasticity. Unexpectedly, this diet also modified the lipidome and dimension of lipid rafts, as well as the domain redistribution of glutamate receptors and acid‐sensing ion channels involved in hippocampal synaptic plasticity, likely modulating functionality of lipid rafts in memory formation and reluctance to age‐associated cognitive decline.
Leveraging complexity frameworks to refine theories of engagement: Advancing self‐regulated learning in the age of artificial intelligence
Authors: Hilpert Jonathan C. , Greene Jeffrey A. , Bernacki Matthew 
Published: May 2023

AbstractCapturing evidence for dynamic changes in self‐regulated learning (SRL) behaviours resulting from interventions is challenging for researchers. In the current study, we identified students who were likely to do poorly in a biology course and those who were likely to do well. Then, we randomly assigned a portion of the students predicted to perform poorly to a science of learning to learn intervention where they were taught SRL study strategies. Learning outcome and log data (257 K events) were collected from n = 226 students. We used a complex systems framework to model the differences in SRL including the amount, interrelatedness, density and regularity of engagement captured in digital trace data (ie, logs). Differences were compared between students who were predicted to (1) perform poorly (control, n = 48), (2) perform poorly and received intervention (treatment, n = 95) and (3) perform well (not flagged, n = 83). Results indicated that the regularity of students' engagement was predictive of course grade, and that the intervention group exhibited increased regularity in engagement over the control group immediately after the intervention and maintained that increase over the course of the semester. We discuss the implications of these findings in relation to the future of artificial intelligence and potential uses for monitoring student learning in online environments.
Practitioner notesWhat is already known about this topic

Self‐regulated learning (SRL) knowledge and skills are strong predictors of postsecondary STEM student success.
SRL is a dynamic, temporal process that leads to purposeful student engagement.
Methods and metrics for measuring dynamic SRL behaviours in learning contexts are needed.
What this paper adds

A Markov process for measuring dynamic SRL processes using log data.
Evidence that dynamic, interaction‐dominant aspects of SRL predict student achievement.
Evidence that SRL processes can be meaningfully impacted through educational intervention.
Implications for theory and practice

Complexity approaches inform theory and measurement of dynamic SRL processes.
Static representations of dynamic SRL processes are promising learning analytics metrics.
Engineered features of LMS usage are valuable contributions to AI models.
Leadership, mobilization of risky behaviours and accountability: The Church of Greece leaders' public talk during the COVID‐19 pandemic
Authors: Ntontis Evangelos , Bozatzis Nikos , Kokkini Vasiliki 
Published: May 2023

AbstractSocial psychologists have typically examined leadership and risk‐taking behaviours through a social identity lens. However, the rhetorical/ideological aspects of such processes as well as leaders' accountability management practices have not been adequately studied. We address this gap by focusing on leaders of the Church of Greece (CoG), who, at the onset of the COVID‐19 pandemic, insisted that their congregation should keep receiving the Holy Communion, which typically involves the practice of spoon‐sharing. We present a discursive analysis of 17 interviews with leaders of the CoG given in Greek media channels, exploring how they construct participation in the ritual. When Church leaders urged their audiences to engage in risky practices, they assumed various social identity positions (e.g. scientifically informed; civic minded), implicating competing ideological frameworks. They also managed their personal and institutional accountability for potential viral transmissions by placing responsibility for adverse effects on their followers. Implications for social psychological theory are discussed.
Pharmacological inhibition of TRPV2 attenuates phagocytosis and lipopolysaccharide‐induced migration of primary macrophages
Authors: Raudszus Rick , Paulig Andrea , Urban Nicole , Deckers Anke , Gräßle Simone , Vanderheiden Sylvia , Jung Nicole , Bräse Stefan , Schaefer Michael , Hill Kerstin 
Published: May 2023

AbstractBackground and PurposeIn macrophages, transient receptor potential vanilloid 2 (TRPV2) channel contributes to various cellular processes such as cytokine production, differentiation, phagocytosis and migration. Due to a lack of selective pharmacological tools, its function in immunological processes is not well understood and the identification of novel and selective TRPV2 modulators is highly desirable.Experimental ApproachNovel and selective TRPV2 modulators were identified by screening a compound library using Ca2+ influx assays with human embryonic kidney 293 (HEK293) cells heterologously expressing rat TRPV2. Hits were further characterized and validated with Ca2+ influx and electrophysiological assays. Phagocytosis and migration of macrophages were analysed and the contribution of TRPV2 to the generation of Ca2+ microdomains was studied by total internal reflection fluorescence microscopy (TIRFM).Key ResultsThe compound IV2‐1, a dithiolane derivative (1,3‐dithiolan‐2‐ylidene)‐4‐methyl‐5‐phenylpentan‐2‐one), is a potent inhibitor of heterologously expressed TRPV2 channels (IC50 = 6.3 ± 0.7 μM) but does not modify TRPV1, TRPV3 or TRPV4 channels. IV2‐1 also inhibits TRPV2‐mediated Ca2+ influx in macrophages. IV2‐1 inhibits macrophage phagocytosis along with valdecoxib and after siRNA‐mediated knockdown. Moreover, TRPV2 inhibition inhibits lipopolysaccharide‐induced migration of macrophages whereas TRPV2 activation promotes migration. After activation, TRPV2 shapes Ca2+ microdomains predominantly at the margin of macrophages, which are important cellular regions to promote phagocytosis and migration.Conclusions and ImplicationsIV2‐1 is a novel TRPV2‐selective blocker and underline the role of TRPV2 in macrophage‐mediated phagocytosis and migration. Furthermore, we provide evidence that TRPV2 activation generates Ca2+ microdomains, which may be involved in phagocytosis and migration of macrophages.