bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of anthropogenic stress on hosts and their microbiomes: Treated wastewater alters performance and gut microbiome of a key detritivore (Asellus aquaticus)
Authors: Lafuente Elvira , Carles Louis , Walser Jean‐Claude , Giulio Marco , Wullschleger Simon , Stamm Christian , Räsänen Katja 
Published: March 2023

AbstractHuman activity is a major driver of ecological and evolutionary change in wild populations and can have diverse effects on eukaryotic organisms as well as on environmental and host‐associated microbial communities. Although host–microbiome interactions can be a major determinant of host fitness, few studies consider the joint responses of hosts and their microbiomes to anthropogenic changes. In freshwater ecosystems, wastewater is a widespread anthropogenic stressor that represents a multifarious environmental perturbation. Here, we experimentally tested the impact of treated wastewater on a keystone host (the freshwater isopod Asellus aquaticus) and its gut microbiome. We used a semi‐natural flume experiment, in combination with 16S rRNA amplicon sequencing, to assess how different concentrations (0%, 30%, and 80%) of nonfiltered wastewater (i.e. with chemical toxicants, nutrients, organic particles, and microbes) versus ultrafiltered wastewater (i.e. only dissolved pollutants and nutrients) affected host survival, growth, and food consumption as well as mid‐ and hindgut bacterial community composition and diversity. Our results show that while host survival was not affected by the treatments, host growth increased and host feeding rate decreased with nonfiltered wastewater – potentially indicating that A. aquaticus fed on organic matter and microbes available in nonfiltered wastewater. Furthermore, even though the midgut microbiome (diversity and composition) was not affected by any of our treatments, nonfiltered wastewater influenced bacterial composition (but not diversity) in the hindgut. Ultrafiltered wastewater, on the other hand, affected both community composition and bacterial diversity in the hindgut, an effect that in our system differed between sexes. While the functional consequences of microbiome changes and their sex specificity are yet to be tested, our results indicate that different components of multifactorial stressors (i.e. different constituents of wastewater) can affect hosts and their microbiome in distinct (even opposing) manners and have a substantial impact on eco‐evolutionary responses to anthropogenic stressors.
Treatment response to isotretinoin correlates with specific shifts in Cutibacterium acnes strain composition within the follicular microbiome
Authors: Nolan Zachary T. , Banerjee Kalins , Cong Zhaoyuan , Gettle Samantha L. , Longenecker Amy L. , Kawasawa Yuka I. , Zaenglein Andrea L. , Thiboutot Diane M. , Agak George W. , Zhan Xiang , Nelson Amanda M. 
Published: March 2023

AbstractThere are no drugs as effective as isotretinoin for acne. Deciphering the changes in the microbiome induced by isotretinoin in the pilosebaceous follicle of successfully treated patients can pave the way to identify novel therapeutic alternatives. We determined how the follicular microbiome changes with isotretinoin and identified which alterations correlate with a successful treatment response. Whole genome sequencing was done on casts from facial follicles of acne patients sampled before, during and after isotretinoin treatment. Alterations in the microbiome were assessed and correlated with treatment response at 20 weeks as defined as a 2‐grade improvement in global assessment score. We investigated the α‐diversity, β‐diversity, relative abundance of individual taxa, Cutibacterium acnes strain composition and bacterial metabolic profiles with a computational approach. We found that increased β‐diversity of the microbiome coincides with a successful treatment response to isotretinoin at 20 weeks. Isotretinoin selectively altered C. acnes strain diversity in SLST A and D clusters, with increased diversity in D1 strains correlating with a successful clinical response. Isotretinoin significantly decreased the prevalence of KEGG Ontology (KO) terms associated with four distinct metabolic pathways inferring that follicular microbes may have limited capacity for growth or survival following treatment. Importantly, these alterations in microbial composition or metabolic profiles were not observed in patients that failed to achieve a successful response at 20 weeks. Alternative approaches to recapitulate this shift in the balance of C. acnes strains and microbiome metabolic function within the follicle may be beneficial in the future treatment of acne.
Telomere length in multiple cancer: insight into recurrence risk from a meta‐analysis
Authors: Gao Mingtong , Zheng Gege , Li Yun , Zhang Yingying , Hu Po , Pan Yang 
Published: March 2023

AbstractBackgroundSeveral studies have revealed the relationships between telomere length and the risk and mortality of numerous cancers. This meta‐analysis aims to insightfully clarify the potential relationship between telomere length and the recurrence of multiple cancers.MethodsPubMed database was used to search and identify interrelated citations. These reports investigated the relationship between telomere length and various cancer recurrences. Meta‐analysis pooled data from studies that reported risk ratio (RR) of 95 (CI = 95%) confidence intervals and/or P‐values. The cancer recurrence was investigated from an overall standpoint to the multiple levels of subtypes of cancers.ResultsThe meta‐analysis involved 5907 recurrent multiple cancer patients from 13 cohort studies. Compared to these cancer recurrence cases and the telomere length differences, there was no significant correlation between telomere length and cancer recurrence risk (short telomeres vs long telomeres; RR = 0.93, 95% CI: 0.72–1.20, P = 0.59). Additionally, a negative association was observed between telomere length and cancer recurrence in gastrointestinal cancer and a positive association in head and neck cancer, while telomere length had little effect on the recurrence of hematological malignancies and genitourinary cancer in this analysis.ConclusionThere was no significant relationship between recurrence and telomere length in 5907 cases in 13 studies. However, there was a correlation between specific tumors. These results suggested that telomere length as a recurrence marker or telomere length to determine the possibility of recurrence must be evaluated on the specific type of cancer.
Divergent contributions of coding and noncoding sequences to initial high‐altitude adaptation in passerine birds endemic to the Qinghai–Tibet Plateau
Authors: Hao Yan , Song Gang , Zhang Yong E. , Zhai Weiwei , Jia Chenxi , Ji Yanzhu , Tang Shiyu , Lv Hongrui , Qu Yanhua , Lei Fumin 
Published: March 2023

AbstractEarly events in the evolution of an ancestral lineage can shape the adaptive patterns of descendant species, but the evolutionary mechanisms driving initial adaptation from an ancestor remain largely unexplored. High‐altitude adaptations have been extensively explored from the viewpoint of protein‐coding genes; however, the contribution of noncoding regions remains relatively neglected. Here, we integrate genomic and transcriptomic data to investigate adaptive evolution in the ancestor of three high‐altitude snowfinch species endemic to the Qinghai–Tibet Plateau. Our genome‐wide scan for adaptation in the snowfinch ancestor identifies strong adaptation signals in functions of development and metabolism for the coding genes, but in functions of the nervous system development for noncoding regions. This pattern is exclusive to the snowfinch ancestor compared to a control ancestral lineage subject to weak selection. Changes in noncoding regions in the snowfinch ancestor, especially those nearest to coding genes, may be disproportionately associated with the differential expression of genes in the brain tissue compared to other tissues. Extensive gene expression in the brain tissue can be further altered via genetic regulatory networks of transcription factors harbouring potential accelerated regulatory regions (e.g., the development‐related transcription factor YEATS4). Altogether, our study provides new evidence concerning how coding and noncoding sequences work through decoupled pathways in initial adaptation to the selective pressure of high‐altitude environments. The analysis highlights the idea that noncoding sequences may be promising elements in facilitating the rapid evolution and adaptation to high altitudes.
Light emission processes in the context of optical beam profile monitors
Authors: Hampf Raphael , Wieser Jochen , Ulrich Andreas 
Published: March 2023

AbstractLight emission from gas targets using heavy ion beam excitation is described. Typically 32S beams with ≈90 MeV (2.8 MeV/amu) particle energy were used. This study was performed in context of optical beam profile measurements. Optical transitions from neutral and singly ionized rare gases are suggested for this application. Emission spectra and their line intensities are presented for a wide range of target pressures from about 10–5 to 300 mbar. The effect of secondary electrons on the beam profiles is discussed. A comparison of ion beam and electron beam-induced spectra is shown and interpreted by a semi-quantitative model of the excitation mechanisms. Examples of beam profiles recorded with three different cameras through appropriate optical filters are presented. A comparison of projected profiles and radial profiles obtained by Abel inversion is given. Effective emission cross sections were measured for atomic and ionic lines at various target gas pressures and their pressure dependence interpreted by the excitation mechanisms. Examples of time-resolved measurements of light emission following pulsed excitation support the interpretation of the excitation mechanisms discussed in this overview of ion beam-induced light emission of gas targets.
Graphical Abstract
Heuristic-Based Ensemble Model Selection Strategy with Parameter Tuning for Optimal Diabetes Mellitus Prediction
Authors: Kulkarni Girish , Manike Chiranjeevi 
Published: March 2023

Diabetes is a terrible health situation characterized by high-rise blood glucose levels. If it is not predicted at an early stage, then it generates the problems in the human body like kidney failure or premature death, and stroke. Controlling blood glucose levels provides patients with helpful dietary recommendations, which are critical components of diabetes management. In the past decades, diverse conventional approaches have been executed to predict the beginning stages of diabetes mellitus depending on physical and substance tests. Still, developing a new framework that can effectively diagnose diabetes mellitus-affected patients is required. To this end, the major target of this task is to predict diabetes mellitus with an advanced accuracy rate with the help of the Heuristic-based Ensemble Model Selection Strategy (H-EMSS). In the data collection phase, the Pima Indian Diabetes dataset (PID) is taken from the storage area of UCI. The data cleaning is performed in the pre-processing stage, which is the technique of removing or fixing, corrupted, incorrect, duplicate, incomplete data, or incorrectly formatted, inside a dataset. Then, the diabetes prediction is accomplished by the H-EMSS. Here, 10 base learners like Naive Bayes (NB), Convolutional Neural Network (CNN), Linear Regression (LR), Deep Neural Network (DNN), Support Vector Machine (SVM), Artificial Neural Network (ANN), Decision Tree (DT), Random Forest (RF), Auto Encoder (AE) and Recurrent Neural Network (RNN) are considered. From these, three classifiers are optimally selected by the Modified Scalar Factor-based Elephant Herding Optimization (MSF-EHO), so that the prediction rate will be high. The suggested methodology’s efficacy is also compared and analyzed, with the findings demonstrating the recommended model’s superiority. The overall evaluation is that the Root Mean Square Error (RMSE) of the designed Modified Scalar Factor-based Elephant Herding Optimization-Heuristic-based Ensemble Model Selection Strategy (MSF-EHO-H-EMSS) attains 4.601% and also the Mean Absolute Error (MAE) on the designed method achieves 0.99%. Thus, the given outcomes of the designed method revealed that it achieves elevated performance than the other existing techniques regarding diverse error metrics.
Supplementary MovieS 1a from Immunotherapeutic Targeting and PET Imaging of DLL3 in Small-Cell Neuroendocrine Prostate Cancer
Authors: Chou Jonathan , Egusa Emily A. , Wang Sinan , Badura Michelle L. , Lee Fei , Bidkar Anil P. , Zhu Jun , Shenoy Tanushree , Trepka Kai , Robinson Troy M. , Steri Veronica , Huang Jiaoti , Wang Yuzhuo , Small Eric J. , Chan Emily , Stohr Bradley A. , Ashworth Alan , Delafontaine Brant , Rottey Sylvie , Cooke Keegan S. , Hashemi Sadraei Nooshin , Yu Brian , Salvati Mark , Bailis Julie M. , Feng Felix Y. , Flavell Robert R. , Aggarwal Rahul 
Published: March 2023

<p>Supplemental Movies 1a-1d: 22Rv1-LacZ and 22Rv1-DLL3 cells co-cultures. Time-lapse movies of 22Rv1-LacZ and 22Rv1-DLL3 cells (labeled with NuclightRed) co-cultured with T cells and either NT control BiTE® or AMG 757. T cells were plated at an E:T ratio of 5:1. Images were captured every 6 hours at 10x magnification.
a.	22Rv1-LacZ cells and T cells treated with NT control BiTE®.
b.	22Rv1-DLL3 cells and T cells treated with NT control BiTE®.
c.	22Rv1-LacZ cells and T cells treated with AMG 757.
d.	22Rv1-DLL3 cells and T cells treated with AMG 757.</p>
Simultaneous Ovarian and Renal Vein Thrombosis as a Rare Cause of Postoperative Pain After Sigmoid Colectomy
Authors: Chandran Tara , Bani Hani Murad , Hain Jon 
Published: March 2023

Post operative pain is often the cause of many ED visits. When patients are discharged and return with postoperative abdominal pain, common etiologies include incisional pain, neuropathic pain, musculoskeletal pain from immobility, ileus, and more sinister causes including adhesive bowel obstruction, abscess formation, and anastomotic leak. We present a 62 year-old female without any hereditary thrombophilia or other prothrombotic factors who presented to the ED after a sigmoid colectomy and diverting ileostomy for perforated diverticulitis and subsequent ileostomy reversal with abdominal pain. CT discovered a left ovarian vein thrombus extending into the left renal vein. With a myriad of diagnoses, it is important to have a low threshold for imaging to rule out serious pathology and also diagnose any unusual causes that can be treated promptly to prevent organ damage and subsequent complications.
Perspectives From Extension Staff on Building and Sustaining Community Wellness Coalitions
Authors: Moore Lindsay , McCormack Lacey , Meendering Jessica R. , Burdette Linda , Stluka Suzanne 
Published: March 2023

Background Community wellness coalitions are an important part of functioning program infrastructure and have been effective in promoting policy, systems, and environmental (PSE) change, especially when paired with technical support by a community champion or Extension staff. PSE strategies are critical to support long-lasting behavior change but can be challenging to implement. Extension is an established and equipped organization that has potential to help community overcome those challenges. The aim of this article was to identify and describe experiences of Extension staff working as community coaches. Methods A mixed-methods design to assess the impact of Extension staff working with Community Champions included a quantitative Extension Coaching Confidence Scorecard and an Extension Key Informant Interview. Results The total Extension Coaching Confidence score significantly increased from pre- to post-intervention (55.1 ± 35.3 vs. 81.7 ± 37.7, p = .03). Five facilitators and two barriers to wellness coalition development were identified by Extension staff. Discussion The results of this study indicate that the model of community coaching used was effective at addressing the foundational components of the Component Model of Infrastructure (CMI). However, comprehensive training for Extension staff in the CMI and technical assistance are needed in order to build capacity, achieve outcomes, and promote sustainability. Implications for Practice Individuals seeking to transition to PSE work should be provided with a foundation of specific, targeted training in the CMI and evidence-based technical assistance methods. Practitioners should recognize the essential role of community champions in PSE work. Completing the Extension Coaching Confidence Scorecard periodically can inform evolving training needs.
Roadblocks and detours on pathways to a clinical diagnosis of autism for girls and women: A qualitative secondary analysis
Authors: Hamdani Yani , Kassee Caroline , Walker Meaghan , Lunsky Yona , Gladstone Brenda , Sawyer Amanda , Ameis Stephanie H , Desarkar Pushpal , Szatmari Peter , Lai Meng-Chuan 
Published: March 2023

Background: Autism is not always considered for girls and women until later along their clinical diagnostic pathways. Misdiagnosis or late diagnosis can pose significant disadvantages with respect to accessing timely health and autism-related services and supports. Understanding what contributes to roadblocks and detours along clinical pathways to an autism diagnosis can shed light on missed opportunities for earlier recognition. Objective: Our objective was to examine what contributed to roadblocks, detours, and missed opportunities for earlier recognition and clinical diagnosis of autism for girls and women. Design: We conducted a qualitative secondary analysis using data from a Canadian primary study that examined the health and healthcare experiences of autistic girls and women through interviews and focus groups. Methods: Transcript data of 22 girls and women clinically diagnosed with autism and 15 parents were analysed, drawing on reflexive thematic analysis procedures. Techniques included coding data both inductively based on descriptions of roadblocks and detours and deductively based on conceptualizations of sex and gender. Patterns of ideas were categorized into themes and the ‘story’ of each theme was refined through writing and discussing analytic memos, reflecting on sex and gender assumptions, and creating a visual map of clinical pathways. Results: Contributing factors to roadblocks, detours, and missed opportunities for earlier recognition and diagnosis were categorized as follows: (1) age of pre-diagnosis ‘red flags’ and ‘signals’; (2) ‘non-autism’ mental health diagnoses first; (3) narrow understandings of autism based on male stereotypes; and (4) unavailable and unaffordable diagnostic services. Conclusion: Professionals providing developmental, mental health, educational, and/or employment supports can be more attuned to nuanced autism presentations. Research in collaboration with autistic girls and women and their childhood caregivers can help to identify examples of nuanced autistic features and how context plays a role in how these are experienced and navigated.
‘I am a Punk, I Don’t Give a Damn!’ Chez Narcisse: A Rural Place of Resistance
Authors: Tuaillon Demésy Audrey 
Published: March 2023

Chez Narcisse is a bar located on the threshold of the southern Vosges, in the east of France, that has been run by the same family for over 120 years. Its particularity lies in the fact that since the mid 1980s, it has included a concert hall in the garden behind the bar, which punk bands use to play. This place brings together a bar that is anchored in the daily life of the village and these festive events, which always take place on Sunday evenings. Chez Narcisse thus appears as a ‘double’ space that emphasizes the search for independence and culture in a rural environment. An ethnographic research carried out in this place since January 2017 highlights how the DIY ethos is as much a way of doing things as a purpose. The punk ethos of DIY is claimed by the owners, volunteers, musicians and stakeholders of Chez Narcisse in order to build its identity as an ‘alternative’ place well known in the contemporary French punk scene. By taking up James C. Scott’s work, the aim is to understand how Chez Narcisse is a social site of resistance and the role that DIY plays in it. To do this, I will first present the construction of this alternative place which articulates both a local and rural level and a global one (the concerts and the venue attract punks from all over France). A performance upstage testifies to a veiled but very present resistance, based on a family imaginary. The second part aims at highlighting the daily forms of resistance at Chez Narcisse expressed through the use of DIY. Thus, this place would be less ‘punk’ through programmed music than through the use of DIY as a value to defend and a project to share.
Factors contributing to preventing operating room “never events”: a machine learning analysis
Authors: Arad Dana , Rosenfeld Ariel , Magnezi Racheli 
Published: March 2023

Abstract
Background
A surgical “Never Event” is a preventable error occurring immediately before, during or immediately following surgery. Various factors contribute to the occurrence of major Never Events, but little is known about their quantified risk in relation to a surgery’s characteristics. Our study uses machine learning to reveal and quantify risk factors with the goal of improving patient safety and quality of care.

Methods
We used data from 9,234 observations on safety standards and 101 root-cause analyses from actual, major “Never Events” including wrong site surgery and retained foreign item, and three random forest supervised machine learning models to identify risk factors. Using a standard 10-cross validation technique, we evaluated the models’ metrics, measuring their impact on the occurrence of the two types of Never Events through Gini impurity.

Results
We identified 24 contributing factors in six surgical departments: two had an impact of > 900% in Urology, Orthopedics, and General Surgery; six had an impact of 0–900% in Gynecology, Urology, and Cardiology; and 17 had an impact of < 0%. Combining factors revealed 15–20 pairs with an increased probability in five departments: Gynecology, 875–1900%; Urology, 1900–2600%; Cardiology, 833–1500%; Orthopedics,1825–4225%; and General Surgery, 2720–13,600%. Five factors affected wrong site surgery’s occurrence (-60.96 to 503.92%) and five affected retained foreign body (-74.65 to 151.43%): two nurses (66.26–87.92%), surgery length < 1 h (85.56–122.91%), and surgery length 1–2 h (-60.96 to 85.56%).

Conclusions
Using machine learning, we could quantify the risk factors’ potential impact on wrong site surgeries and retained foreign items in relation to a surgery’s characteristics, suggesting that safety standards should be adjusted to surgery’s characteristics based on risk assessment in each operating room. .

Trial registration number
MOH 032-2019.