bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histological Analysis of Regenerative Properties in Human Glenoid Labral Regions
Authors: Hoang Le Q. , Vaish Bhavya , Izuagbe Samira , Co Cynthia M. , Borrelli Joseph , Millett Peter J. , Tang Liping 
Published: May 2023

Background: The healing capacity of the human glenoid labrum varies by tear location. Current evidence suggests that the healing capacity of meniscal and cartilage injuries relates to cellular composition and vascularity. However, little is known about the histological characteristics of the glenoid labrum and how they may affect healing potential in specific anatomic regions. Hypothesis: Regenerative characteristics of the glenoid labrum differ based on the anatomic region. Study Design: Descriptive laboratory study. Methods: Human glenoid labra from fresh unpreserved cadavers were transversely sectioned in different anatomic regions. Masson trichrome stain was used to determine dense and loose extracellular matrix regions and vessel densities. Hematoxylin and eosin, Ki-67+, and CD90+/CD105+ stains were performed to determine total, proliferative, and progenitor cell densities, respectively. Regression models demonstrated relationships between vascular area, progenitor cell quantity, and probability of successful operation. Results: Among all labral aspects, the superior glenoid labrum had the highest percentage (56.8% ± 6.9%) of dense extracellular matrix or avascular tissue ( P < .1). The vascular region of the superior labrum had the fewest total cells (321 ± 135 cells/mm2; P < .01) and progenitor cells (20 ± 4 cells/mm2; P < .001). Vascular area was directly correlated with progenitor cell quantity ( P = .006002). An increase in probability of successful operation was associated with a linear increase in vascular area ( R2 = 0.765) and an exponential increase in progenitor cell quantity ( R2 = 0.795). Subsequently, quadratic models of vascularity and progenitor cell quantity around the labral clock were used to assess relative healing potential. Quadratic models for percentage vascular area ( P = 6.35e-07) and weighted progenitor cell density ( P = 3.03e-05) around the labral clock showed that percentage vascular area and progenitor cell quantity increased as labral tissue neared the inferior aspect and diminished near the superior aspect. Conclusion: Anatomic regions of the glenoid labrum differ in extracellular matrix composition, vascularity, and cell composition. The superior glenoid labrum is deficient in vascularity and progenitor cells, which may explain the high failure rates for repairs in this location. Clinical Relevance: Improved understanding of the composition of distinct glenoid labral positions may help to improve therapeutic strategies for labral pathology.
A Scoping Review of Workplace Culture of Health Measures
Authors: Marenus Michele W. , Marzec Mary , Chen Weiyun 
Published: May 2023

Objective This study aimed to review existing measures of workplace culture of health and to examine the health and wellbeing outcomes associated with workplace culture of health. Data Sources PubMed/Medline, Web of Science, and PsycINFO databases searched through February 2022. Study Inclusion and Exclusion Criteria Articles were included if they used a specific measure to assess culture of health in the workplace and were published in English. Articles were excluded if there was no quantitative measure of health culture. Data Extraction Data from each article was extracted using a structured template which included study purpose, participants and setting, study design, intervention strategies (if applicable), culture of health measure, and results. Data Synthesis We described culture of health measures used and summarized key findings from included articles. Results The search yielded 31 articles measuring workplace culture of health (three validation, two intervention, and 26 observational studies). Nineteen unique measures were used across all articles. Most studies examined culture of health from the employee perspective (n = 23), while others examined it at the organizational level (n = 7). The studies indicated a positive relationship between health and well-being outcomes and a strong workplace health culture. Conclusion There are many different approaches to measuring workplace health culture. Overall, workplace culture of health is related to positive employee and organizational health and wellbeing outcomes.
Sars-CoV2 infection in pregnant women with multiple sclerosis
Authors: Aprea Maria Grazia , Schiavetti Irene , Portaccio Emilio , Ballerini Chiara , Battaglia Mario Alberto , Bergamaschi Roberto , Brichetto Giampaolo , Bunul S Destan , Calabrese Massimiliano , Capobianco Marco , Cavalla Paola , Celani Maria Grazia , Clerico Marinella , Cocco Eleonora , Comi Giancarlo , Confalonieri Paolo , Conte Antonella , Cordioli Cinzia , De Luca Giovanna , De Rossi Nicola , Filippi Massimo , Gumes Haluk , Immovilli Paolo , Inglese Matilde , Karabudak Rana , Landi Doriana , Lanzillo Roberta , L’Episcopo Maria Rita , Lorefice Lorena , Mantero Vittorio , Marangoni Sabrina , Marfia Girolama Alessandra , Masciulli Camilla , Milano Eva , Moiola Lucia , Orlandi Riccardo , Patti Francesco , Perini Paola , Pesci Ilaria , Pucci Eugenio , Puthenparampil Marco , Radaelli Marta , Salvetti Marco , Sartori Arianna , Scandellari Cinzia , Sen Sedat , Siva Aksel , Strumia Silvia , Teatini Francesco , Tedeschi Gioacchino , Trojano Maria , Tutuncu Melih , Vaula Giovanna , Sormani Maria Pia , Amato Maria Pia ,   Musc-19 Study Group
Published: May 2023

Background: In the general population, maternal SARS-CoV-2 infection during pregnancy is associated with worse maternal outcomes; however, only one study so far has evaluated COVID-19 clinical outcomes in pregnant and postpartum women with multiple sclerosis, showing no higher risk for poor COVID-19 outcomes in these patients. Objective: In this multicenter study, we aimed to evaluate COVID-19 clinical outcomes in pregnant patients with multiple sclerosis. Methods: We recruited 85 pregnant patients with multiple sclerosis who contracted COVID-19 after conception and were prospectively followed-up in Italian and Turkish Centers, in the period 2020-2022. A control group of 1354 women was extracted from the database of the Multiple Sclerosis and COVID-19 (MuSC-19). Univariate and subsequent logistic regression models were fitted to search for risk factors associated with severe COVID-19 course (at least one outcome among hospitalization, intensive care unit [ICU] admission and death). Results: In the multivariable analysis, independent predictors of severe COVID-19 were age, body mass index ⩾ 30, treatment with anti-CD20 and recent use of methylprednisolone. Vaccination before infection was a protective factor. Vaccination before infection was a protective factor. Pregnancy was not a risk nor a protective factor for severe COVID-19 course. Conclusion: Our data show no significant increase of severe COVID-19 outcomes in patients with multiple sclerosis who contracted the infection during pregnancy.
Surgical video use and preparation for operative procedures among urology residents and fellows: Results from a national survey
Authors: Movassaghi Miyad , Garza Erick A , Badalato Gina M , Chung Doreen E 
Published: May 2023

Objective: The availability of surgical videos has changed the nature of learning outside the operating room. Within urology, there are limited reports on the use of surgical videos during training. This study sought to characterise both the preferred platforms and the utilization patterns of videos among a contemporary cohort of urology trainees. Materials and Methods: An anonymous survey was distributed by the Society of Academic Urologists to trainees in September 2021. Data prospectively collected included respondent demographics, video platforms utilized, perceived educational value, satisfaction, and overall time spent using videos for case preparation. Results: In total, 169 urology residents and fellows completed the survey (23.6%). Nearly all (96.4%) respondents reported utilizing videos to prepare for cases, with 95.1% reporting videos as helpful for this purpose. YouTube (81.4%) and American Urological Association (AUA) University (16.3%) were the most common sources for video content. Video ‘narration’ was the most valued feature. When stratified by trainee level, 91.5% of senior residents/fellows reported using YouTube, compared with 56.6% of junior residents ( p < 0.005). Overall, approximately 4 out of 5 trainees ultimately recommend YouTube as a video resource, compared with 57% recommending AUA University ( p < 0.05). Conclusion: Most urology trainees report surgical videos as a crucial component of their training, preferring to access videos directly from the YouTube website. As the quality of videos from YouTube varies, finding ways to share quality educational content should remain a priority for the medical education community moving forward. Level of evidence: Not applicable
Creation of 21st century anatomy facilities: designing facilities for integrated preclinical education in the Middle East
Authors: Lorke Dietrich E , Rock John A. , Hernandez Robert , Graham David , Keough Natalie , van Tonder Daniël J. 
Published: May 2023

Abstract
Background
The establishment of new anatomy facilities needs to accommodate a combination of modern teaching modalities that best align with evidence-based best teaching practices. This article describes the process in which our state-of-the-art anatomy laboratories were designed and implemented, and how these facilities support aspects of modern anatomy education.

Methods
A list of best practices for anatomy education in a modern medical curriculum was summarized from the literature. To assess student satisfaction, a survey related to student perception of the anatomy facilities (5-point Likert scale) was conducted.

Results
Our educational modalities include a broad range of teaching approaches. The Instructional Studio houses prosected and plastinated specimens, and cadaveric dissections are performed. Each of our three Dry Laboratories allow for active learning and interaction between small student groups. The Webinar Room acts as a conference room for departmental and online meetings, discussions with students, and dialogues with affiliated hospitals via the internet. The Imaging Center is equipped with a Sectra® medical educational platform, CAE Vimedix® Virtual Medical Imaging Ultrasound Training System, and Philipps Lumify® Ultrasound devices to train students to conduct and interpret sonographic images. Moreover, the Complete Anatomy® program is made available to all our students.

Conclusion
The layout of our newly created Anatomy Facilities allows for all aspects of modern medical education mentioned in the literature. These educational modalities and teaching approaches are highly appreciated by our faculty and students. Moreover, these technologies allowed for a smooth transition from on-site anatomy teaching to online education during the COVID pandemic.
Exploring the prognostic function of TMB-related prognostic signature in patients with colon cancer
Authors: Zhao Yan , Liang Xiaolong , Duan Xudong , Zhang Chengli 
Published: May 2023

AbstractTumor mutation burden (TMB) level is identified as a useful predictor in multiple tumors including colon adenocarcinoma (COAD). However, the function of TMB related genes has not been explored previously. In this study, we obtained patients’ expression and clinical data from The Cancer Genome Atlas (TCGA) and the National Center for Biotechnology Information (NCBI). TMB genes were screened and subjected to differential expression analysis. Univariate Cox and LASSO analyses were utilized to construct the prognostic signature. The efficiency of the signature was tested by using a receiver operating characteristic (ROC) curve. A nomogram was further plotted to assess the overall survival (OS) time of patients with COAD. In addition, we compared the predictive performance of our signature with other four published signatures. Functional analyses indicated that patients in the low-risk group have obviously different enrichment of tumor related pathways and tumor infiltrating immune cells from that of high-risk patients. Our findings suggested that the ten genes’ prognostic signature could exert undeniable prognostic functions in patients with COAD, which might provide significant clues for the development of personalized management of these patients.
Mouse embryonic stem cell-derived blood–brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis
Authors: Jezierski Anna , Huang Jez , Haqqani Arsalan S. , Haukenfrers Julie , Liu Ziying , Baumann Ewa , Sodja Caroline , Charlebois Claudie , Delaney Christie E. , Star Alexandra T. , Liu Qing , Stanimirovic Danica B. 
Published: May 2023

AbstractBlood brain barrier (BBB) models in vitro are an important tool to aid in the pre-clinical evaluation and selection of BBB-crossing therapeutics. Stem cell derived BBB models have recently demonstrated a substantial advantage over primary and immortalized brain endothelial cells (BECs) for BBB modeling. Coupled with recent discoveries highlighting significant species differences in the expression and function of key BBB transporters, the field is in need of robust, species-specific BBB models for improved translational predictability. We have developed a mouse BBB model, composed of mouse embryonic stem cell (mESC-D3)-derived brain endothelial-like cells (mBECs), employing a directed monolayer differentiation strategy. Although the mBECs showed a mixed endothelial-epithelial phenotype, they exhibited high transendothelial electrical resistance, inducible by retinoic acid treatment up to 400 Ω cm2. This tight cell barrier resulted in restricted sodium fluorescein permeability (1.7 × 10–5 cm/min), significantly lower than that of bEnd.3 cells (1.02 × 10–3 cm/min) and comparable to human induced pluripotent stem cell (iPSC)-derived BECs (2.0 × 10–5 cm/min). The mBECs expressed tight junction proteins, polarized and functional P-gp efflux transporter and receptor mediated transcytosis (RMT) receptors; collectively important criteria for studying barrier regulation and drug delivery applications in the CNS. In this study, we compared transport of a panel of antibodies binding species selective or cross-reactive epitopes on BBB RMT receptors in both the mBEC and human iPSC-derived BEC model, to demonstrate discrimination of species-specific BBB transport mechanisms.
1-year survival in haemophagocytic lymphohistiocytosis: a nationwide cohort study from England 2003–2018
Authors: West Joe , Stilwell Peter , Liu Hanhua , Ban Lu , Bythell Mary , Card Tim , Lanyon Peter , Nanduri Vasanta , Rankin Judith , Bishton Mark , Crooks Colin 
Published: May 2023

AbstractHaemophagocytic lymphohistiocytosis (HLH) is a lethal syndrome of excessive immune activation. We undertook a nationwide study in England of all cases of HLH diagnosed between 2003 and 2018, using linked electronic health data from hospital admissions and death certification. We modelled interactions between demographics and comorbidities and estimated one-year survival by calendar year, age group, gender and comorbidity (haematological malignancy, auto-immune, other malignancy) using Cox regression. There were 1628 people with HLH identified. Overall, crude one-year survival was 50% (95% Confidence interval 48–53%) which varied substantially with age (0–4: 61%; 5–14: 76%; 15–54: 61%; > 55: 24% p < 0.01), sex (males, 46%, worse than females, 55% p < 0.01) and associated comorbidity (auto-immune, 69%, haematological malignancy 28%, any other malignancy, 37% p < 0.01). Those aged < 54 years had a threefold increased risk of death at 1-year amongst HLH associated with malignancy compared to auto-immune. However, predicted 1-year survival decreased markedly with age in those with auto-immune (age 0–14, 84%; 15–54, 73%; > 55, 27%) such that among those > 55 years, survival was as poor as for patients with haematological malignancy. One-year survival following a diagnosis of HLH varies considerably by age, gender and associated comorbidity. Survival was better in those with auto-immune diseases among the young and middle age groups compared to those with an underlying malignancy, whereas in older age groups survival was uniformly poor regardless of the underlying disease process.
A deep learning model enables accurate prediction and quantification of pulmonary edema from chest X-rays
Authors: Schulz Dominik , Rasch Sebastian , Heilmaier Markus , Abbassi Rami , Poszler Alexander , Ulrich Jörg , Steinhardt Manuel , Kaissis Georgios A. , Schmid Roland M. , Braren Rickmer , Lahmer Tobias 
Published: May 2023

Abstract
Background
A quantitative assessment of pulmonary edema is important because the clinical severity can range from mild impairment to life threatening. A quantitative surrogate measure, although invasive, for pulmonary edema is the extravascular lung water index (EVLWI) extracted from the transpulmonary thermodilution (TPTD). Severity of edema from chest X-rays, to date is based on the subjective classification of radiologists. In this work, we use machine learning to quantitatively predict the severity of pulmonary edema from chest radiography.

Methods
We retrospectively included 471 X-rays from 431 patients who underwent chest radiography and TPTD measurement within 24 h at our intensive care unit. The EVLWI extracted from the TPTD was used as a quantitative measure for pulmonary edema. We used a deep learning approach and binned the data into two, three, four and five classes increasing the resolution of the EVLWI prediction from the X-rays.

Results
The accuracy, area under the receiver operating characteristic curve (AUROC) and Mathews correlation coefficient (MCC) in the binary classification models (EVLWI < 15, ≥ 15) were 0.93 (accuracy), 0.98 (AUROC) and 0.86(MCC). In the three multiclass models, the accuracy ranged between 0.90 and 0.95, the AUROC between 0.97 and 0.99 and the MCC between 0.86 and 0.92.

Conclusion
Deep learning can quantify pulmonary edema as measured by EVLWI with high accuracy.
Histone lysine methyltransferase SMYD3 promotes oral squamous cell carcinoma tumorigenesis via H3K4me3-mediated HMGA2 transcription
Authors: Yang Zongcheng , Liu Fen , Li Zongkai , Liu Nianping , Yao Xinfeng , Zhou Yu , Zhang Liyu , Jiang Pan , Liu Honghong , Kong Lingming , Lang Chuandong , Xu Xin , Jia Jihui , Nakajima Takahito , Gu Wenchao , Zheng Lixin , Zhang Zhihong 
Published: May 2023

Abstract
Background
Epigenetic dysregulation is essential to the tumorigenesis of oral squamous cell carcinoma (OSCC). SET and MYND domain-containing protein 3 (SMYD3), a histone lysine methyltransferase, is implicated in gene transcription regulation and tumor development. However, the roles of SMYD3 in OSCC initiation are not fully understood. The present study investigated the biological functions and mechanisms involved in the SMYD3-mediated tumorigenesis of OSCC utilizing bioinformatic approaches and validation assays with the aim of informing the development of targeted therapies for OSCC.

Results
429 chromatin regulators were screened by a machine learning approach and aberrant expression of SMYD3 was found to be closely associated with OSCC formation and poor prognosis. Data profiling of single-cell and tissue demonstrated that upregulated SMYD3 significantly correlated with aggressive clinicopathological features of OSCC. Alterations in copy number and DNA methylation patterns may contribute to SMYD3 overexpression. Functional experimental results suggested that SMYD3 enhanced cancer cell stemness and proliferation in vitro and tumor growth in vivo. SMYD3 was observed to bind to the High Mobility Group AT-Hook 2 (HMGA2) promoter and elevated tri-methylation of histone H3 lysine 4 at the corresponding site was responsible for transactivating HMGA2. SMYD3 also was positively linked to HMGA2 expression in OSCC samples. Furthermore, treatment with the SMYD3 chemical inhibitor BCI-121 exerted anti-tumor effects.

Conclusions
Histone methyltransferase activity and transcription-potentiating function of SMYD3 were found to be essential for tumorigenesis and the SMYD3–HMGA2 is a potential therapeutic target in OSCC.
Metformin promotes female germline stem cell proliferation by upregulating Gata-binding protein 2 with histone β-hydroxybutyrylation
Authors: Wang Xiang , Tian Geng G. , Cheng Weiwei , Yu Xiaoli , Li Xiaoyong , Wu Ji 
Published: May 2023

AbstractBackgroundMetformin as a first-line clinical anti-diabetic agent prolongs the lifespan of model animals and promotes cell proliferation. However, the molecular mechanisms underlying the proliferative phenotype, especially in epigenetics, have rarely been reported. The aim of this study was to investigate the physiological effects of metformin on female germline stem cells (FGSCs) in vivo and in vitro, uncover β-hydroxybutyrylation epigenetic modification roles of metformin and identify the mechanism of histone H2B Lys5 β-hydroxybutyrylation (H2BK5bhb) in Gata-binding protein 2 (Gata2)-mediated proliferation promotion of FGSCs.MethodsThe physiological effects of metformin were evaluated by intraperitoneal injection and histomorphology. The phenotype and mechanism studies were explored by cell counting, cell viability, cell proliferation assay and protein modification omics, transcriptomics, chromatin immunoprecipitation sequencing in FGSCs in vitro.ResultsWe found that metformin treatment increased the number of FGSCs, promoted follicular development in mouse ovaries and enhanced the proliferative activity of FGSCs in vitro. Quantitative omics analysis of protein modifications revealed that H2BK5bhb was increased after metformin treatment of FGSCs. In combination with H2BK5bhb chromatin immunoprecipitation and transcriptome sequencing, we found that Gata2 might be a target gene for metformin to regulate FGSC development. Subsequent experiments showed that Gata2 promoted FGSC proliferation.ConclusionOur results provide novel mechanistic understanding of metformin in FGSCs by combining histone epigenetics and phenotypic analyses, which highlight the role of the metformin-H2BK5bhb-Gata2 pathway in cell fate determination and regulation.
TSA-PACT: a method for tissue clearing and immunofluorescence staining on zebrafish brain with improved sensitivity, specificity and stability
Authors: Wang Kang , Yu Yuxin , Xu Yinhui , Yue Yingzi , Zhao Fang , Feng Wenyang , Duan Yijie , Duan Weicheng , Yue Jingjing , Liao Zhiyun , Fei Peng , Sun Hui , Xiong Bo 
Published: May 2023

AbstractFor comprehensive studies of the brain structure and function, fluorescence imaging of the whole brain is essential. It requires large-scale volumetric imaging in cellular or molecular resolution, which could be quite challenging. Recent advances in tissue clearing technology (e.g. CLARITY, PACT) provide new solutions by homogenizing the refractive index of the samples to create transparency. However, it has been difficult to acquire high quality results through immunofluorescence (IF) staining on the cleared samples. To address this issue, we developed TSA-PACT, a method combining tyramide signal amplification (TSA) and PACT, to transform samples into hydrogel polymerization frameworks with covalent fluorescent biomarkers assembled. We show that TSA-PACT is able to reduce the opacity of the zebrafish brain by more than 90% with well-preserved structure. Compared to traditional method, TSA-PACT achieves approximately tenfold signal amplification and twofold improvement in signal-to-noise ratio (SNR). Moreover, both the structure and the fluorescent signal persist for at least 16 months with excellent signal retention ratio. Overall, this method improves immunofluorescence signal sensitivity, specificity and stability in the whole brain of juvenile and adult zebrafish, which is applicable for fine structural analysis, neural circuit mapping and three-dimensional cell counting.