bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gluten-sensitive enteropathy in recessive dystrophic epidermolysis bullosa
Authors: Sacedón Rosa , de Arriba M Carmen , Martínez-Santamaría Lucía , Maseda Rocío , Herráiz-Gil Sara , Jiménez Eva , Rosales Isabel , Quintana Lucía , Illera Nuria , García Marta , Butta Nora , Fernández-Bello Ihosvany , Lwin Su M , Fernández-Arquero Miguel , León Carlos , McGrath John A , Vicente M Ángeles , del Río Marcela , de Lucas Raúl , Sánchez-Ramón Silvia , Escámez María José 
Published: September 2023

Recessive dystrophic epidermolysis bullosa (RDEB) is a blistering genodermatosis due to biallelic loss-of-function variants in the type VII collagen (C7) gene (COL7A1). We report the impact of inflammation/autoimmunity on the gut (and other organs) in the nine children with RDEB recruited to an early-phase clinical trial of systemic cell therapy (NCT04153630). This pilot study provides evidence that autoimmunity may play an important role in sustaining chronic inflammation and the coexistence of coeliac disease, which, in turn, could exacerbate anaemia/malnutrition and progression in RDEB. Testing this hypothesis in a larger cohort including children and adults with RDEB and other epidermolysis bullosa (EB) subtypes is warranted so that targeted interventions may improve outcomes.
Lessons learned from the EACTS-MSTCVS quality fellowship: a call to action for continuous improvement of cardiothoracic surgery outcomes in Europe
Authors: Topcu Ahmet Can , Magouliotis Dimitrios E , Milojevic Milan , Bond Chris J , Clark Melissa J , Theurer Patricia F , Pagani Francis D , Pruitt Andrew L , Prager Richard L 
Published: September 2023

Abstract
The Michigan Society of Thoracic and Cardiovascular Surgeons (MSTCVS), a pioneer in initiating and nurturing quality improvement strategies in statewide cardiothoracic surgery, has been running the Quality Collaborative (MSTCVS-QC) program since 2001. This initiative has significantly grown over the years, facilitating at least 4 in-person meetings annually. It actively engages cardiac and general thoracic surgeons, data managers and researchers from all 32 non-federally funded cardiothoracic surgery sites across Michigan. Broadening its influence on joint learning and clinical outcomes, the MSTCVS-QC formed a strategic partnership with Blue Cross Blue Shield of Michigan, the state's largest private insurer, to further promote its initiatives. The MSTCVS-QC, operating from a dedicated QC centre employs an STS-associated database with additional aspects for data collection and analysis. The QC centre also organizes audits, facilitates collaborative meetings, disseminates surgical outcomes and champions the development and implementation of quality improvement initiatives related to cardiothoracic surgery in Michigan. Recognizing the MSTCVS-QC's successful efforts in advancing quality improvement, the European Association for Cardiothoracic Surgery (EACTS) introduced a fellowship program in 2018, facilitated through the EACTS Francis Fontan Fund (FFF). This program allows early-career academic physicians to spend 4–6 months with the MSTCVS-QC team in Ann Arbor. This article chronicles the evolution and functionality of the MSTCVS-QC, enriched by the experiences of the inaugural 4 EACTS/FFF fellows. Our objective is to emphasize the critical importance of fostering a culture of quality improvement and patient safety in the field of cardiothoracic surgery with open discussion of audited, high-quality data points. This principle, while implemented locally, has implications and value extending far beyond Europe, resonating globally.
General practitioners’ perceptions of dealing with patients with pressured speech: a qualitative study
Authors: Lallier Stéphanie , Hourantier Christelle , Gilles de la Londe Julie , Billon Grégoire , Cadwallader Jean-Sébastien , Piot Marie-Aude 
Published: September 2023

Abstract

Background
Empathetic listening can be particularly challenging with patients presenting pressured speech, while time pressure and costs limit practitioners’ availability. We aimed to explore general practitioners’ (GPs’) experiences when encountering a patient with pressured speech without pathological syndrome.

Methods
Using a thematic analysis method, 19 semi-structured interviews were conducted with GPs purposively sampled in Ile-de-France region.

Results
Three themes emerged. First, misgivings were aroused when meeting a patient with pressured speech, including frustration, displeasure, and a struggle to maintain focus. Second, huge efforts were needed to adhere to the appropriate clinical reasoning and care for these patients who need more time and energy. The very definition of this condition varies from one clinician to another, and for some, psychiatric conditions were systematically associated. Third, implementation of specific strategies was required to adjust to these patients: trying to create distance with the patient, to organize a framework in which the patient with pressured speech can express him or herself, and even strategies to increase GPs’ availability.

Conclusion
The time and energy required to provide adequate care for patients presenting with pressured speech make it quite challenging, and this builds up pressure in the waiting room. But when medical demands take this form, there is a risk that clinicians will reject the patient or refer early to mental health services, leading to growing feelings of abandonment, the neglect of organic issues, and medical nomadism. Initial and continuing medical training focussing on advanced communication skills and multifocal approaches can foster long-term follow-up.
The collapse and re-establishment of stability regulate the gradual transition of bacterial communities from macrophytes- to phytoplankton-dominated types in a large eutrophic lake
Authors: Hu Yang , Cai Jian , Gong Ying , Liu Changqing , Jiang Xingyu , Tang Xiangming , Shao Keqiang , Gao Guang 
Published: September 2023

AbstractEutrophic lakes often exhibit two alternative types: macrophytes-dominated (MD) and phytoplankton-dominated (PD). However, the nature of bacterial community types that whether the transition from the MD to the PD types occurs in a gradual or abrupt manner remains hotly debated. Further, the theoretical recognition that stability regulates the transition of bacterial community types remains qualitative. To address these issues, we divided the transition of bacterial communities along a trophic gradient into 12 successional stages, ranging from the MD to the PD types. Results showed that 12 states were clustered into three distinct regimes: MD type, intermediate transitional type and PD type. Bacterial communities were not different between consecutive stages, suggesting that the transition of alternative types occurs in a continuous gradient. At the same time, the stability of bacterial communities was significantly lower in the intermediate type than in the MD or PD types, highlighting that the collapse and re-establishment of community stability regulate the transition. Further, our results showed that the high complexity of taxon interactions and strong stochastic processes disrupt the stability. Ultimately, this study enables deeper insights into understanding the alternative types of microbial communities in the view of community stability.
Unravelling the gut-lung axis: insights into microbiome interactions and Traditional Indian Medicine's perspective on optimal health
Authors: Haldar Swati , Jadhav Snehal R , Gulati Vandana , Beale David J , Balkrishna Acharya , Varshney Anurag , Palombo Enzo A , Karpe Avinash V , Shah Rohan M 
Published: September 2023

Abstract
The microbiome of the human gut is a complex assemblage of microorganisms that are in a symbiotic relationship with one another and profoundly influence every aspect of human health. According to converging evidence, the human gut is a nodal point for the physiological performance matrixes of the vital organs on several axes (i.e. gut-brain, gut-lung, etc). As a result of COVID-19, the importance of gut-lung dysbiosis (balance or imbalance) has been realised. In view of this, it is of utmost importance to develop a comprehensive understanding of the microbiome, as well as its dysbiosis. In this review, we provide an overview of the gut-lung axial microbiome and its importance in maintaining optimal health. Human populations have successfully adapted to geophysical conditions through traditional dietary practices from around the world. In this context, a section has been devoted to the traditional Indian system of medicine and its theories and practices regarding the maintenance of optimally customized gut health.
Crustal and upper mantle structure beneath SE China from joint analysis of receiver functions and Rayleigh-wave dispersion
Authors: Fan Jie , Bao Xuewei , Zhang Bingfeng 
Published: September 2023

SUMMARY
Various geodynamic models, such as a mantle plume, lithospheric delamination and the subduction of palaeo-Pacific Plate, have been proposed to account for the Mesozoic magmatic activity and related mineralization in SE China. However, previous seismic velocity models are insufficient in resolution to constrain the accompanying crust–mantle interactions, which limits our understanding of the deep geodynamic processes responsible for the Mesozoic magmatism in SE China. In this study, we construct a new 3-D shear wave velocity model of the crust and upper mantle beneath SE China using joint inversion of receiver functions and Rayleigh-wave dispersion. We also determine the crustal thicknesses and Vp/Vs ratios by H–k stacking of receiver functions. Compared to the western Cathaysia Block, the coastal volcanic-intrusive belt (CVIB) southeast of the Zhenghe-Dapu Fault shows relatively high-velocity anomalies in the lower crust and high Vp/Vs ratios, indicating significant underplating of mafic material beneath the CVIB. Furthermore, it is noticeable that the asthenospheric low-velocity anomalies are stronger beneath the CVIB than beneath the interior of SE China, suggesting a southeastward increase in the intensity of asthenospheric upwelling and partial melting, which was likely responsible for the strong volcanism and the inferred mafic underplating beneath the CVIB. Our observations provide new evidence and more details for the northwestward subduction and subsequent rollback of the palaeo-Pacific Plate causing the Late Mesozoic magmatism in SE China.
RNA-sequencing analysis reveals novel genes involved in the different peel color formation in eggplant
Authors: Li Jing , Jiang Senlin , Yang Guobin , Xu Yanwei , Li Lujun , Yang Fengjuan 
Published: September 2023

Abstract
Eggplant (Solanum melongena L.) is a highly nutritious vegetable. Here, the molecular mechanism of color formation in eggplants was determined using six eggplant cultivars with different peel colors and two SmMYB113-overexpressing transgenic eggplants with a purple peel and pulp. Significant differentially expressed genes (DEGs) were identified by RNA-sequencing analysis using the following criteria: log2(sample1/sample2) ≥ 0.75 and q-value ≤ 0.05. Two analytical strategies were used to identify genes related to the different peel color according to the peel color, flavonoids content, delphinidins/flavonoids ratio, and the content of anthocyanins. Finally, 27 novel genes were identified to be related to the color difference among eggplant peels and 32 novel genes were identified to be related to anthocyanin biosynthesis and regulated by SmMYB113. Venn analysis revealed that SmCytb5, SmGST, SmMATE, SmASAT3, and SmF3′5’M were shared among both sets of novel genes. Transient expression assay in tobacco suggested that these five genes were not sufficient for inducing anthocyanin biosynthesis alone, but they play important roles in anthocyanin accumulation in eggplant peels. Yeast one-hybrid, electrophoretic mobility shift assay and dual-luciferase assays indicated that the expression of the five genes could be directly activated by SmMYB113 protein. Finally, a regulatory model for the mechanism of color formation in eggplant was proposed. Overall, the results of this study provide useful information that enhances our understanding of the molecular mechanism underlying the different color formation in eggplant.
Modelling and in situ observation of broadband acoustic scattering from the Silver cyprinid (Rastrineobola argentea) in Lake Victoria, East Africa
Authors: Yang Yang , Gastauer Sven , Proud Roland , Mangeni-Sande Richard , Everson Inigo , Kayanda Robert J , Brierley Andrew S 
Published: September 2023

Abstract
Lake Victoria is the second-largest freshwater lake in the world, and fish from the lake are a vital food resource for millions of people living around it. The silver cyprinid (Rastrineobola argentea), a small schooling pelagic species known in Tanzania as “dagaa” contributes ca. 55% to the total annual catch (ca. 0.51 million tonnes (MT) in 2014). The acoustic target strength (TS, dB re 1 m2) of dagaa, a key factor for biomass estimation, is however not well described, and is a major source of uncertainty in biomass estimation. In this study, we developed a Kirchhoff-ray mode (KRM) model to predict the TS of dagaa at standard fisheries survey frequencies. The model was based on the morphology of the body and the dual-chambered swimbladder, as obtained from X-ray images of fish ranging in total length (TL) between 2.8 and 5.4 cm. The results suggested that the swimbladder (which comprises 2.6 to 8.2% of body volume) accounts for ca. 65 to 90% of the total backscattering at 120 kHz. The predicted TS was highly dependent on tilt angle, varying by 14.0 dB at 120 kHz across the tilt range 65–115˚ (where 0˚ is head up and 180˚ is tail up), and TS variability with tilt generally increased with increasing frequency. The tilt angle of acoustically tracked individual fish indicated a distribution of tilt angles with a mean and s.d. of 93.5 and 15.1°. Our model suggested a new tilt-averaged TS–TL relationship for dagaa [$T{S}_{120\ kHz} = 19.49\log ( {TL} ) - 70.3$], which leads to a TS 1.5 dB higher than the value in the relationship presently used to estimate stock biomass. The new relationship will lead to a substantial reduction (by ca. 30%) in estimated biomass. The discrepancies between the mean relative frequency response of the in situ measurements of backscatter from dagaa and the KRM model predictions were in the range of -2.9–3.1 dB at frequencies from 45 to 250 kHz. The KRM modelling and in situ broadband measurements of dagaa will be beneficial for acoustic identification and behavioural studies of dagaa, and will enable improved biomass assessment, thereby underpinning sustainable long-term management.
Interplay of Ebola Virus With Immune Cells Leading to Their Death by Diverse Mechanisms
Authors: Iampietro Mathieu , Amurri Lucia , Reynard Olivier , Bukreyev Alexander 
Published: September 2023

Abstract
Inflammation and cytopenia are commonly observed during Ebola virus (EBOV) infection; however, mechanisms responsible for EBOV-induced cell death remain obscure. While apoptosis and necrosis are already identified as mechanisms of cell death induced by the virus, our study demonstrates that THP-1 monocytes and SupT1 T cells exposed to EBOV undergo pyroptosis and necroptosis, respectively, through a direct contact with EBOV, and also mediate pyroptosis or necroptosis of uninfected bystander cells via indirect effects associated with secreted soluble factors. These results emphasize novel aspects of interactions between EBOV and immune cell populations and provide a better understanding of the immunopathogenesis of EBOV disease.
Identification of fabclavine derivatives, Fcl-7 and Fcl-8, from Xenorhabdus budapestensis as major antifungal natural products against Rhizoctonia solani
Authors: Yuan Baoming , Li Beibei , Shen Hongfei , Duan Jiaqi , Jia Fenglian , Maimaiti Yushanjiang , Li Yaning , Li Guangyue 
Published: September 2023

Abstract

Aims
Black scurf disease, caused by Rhizoctonia solani, is a severe soil-borne and tuber-borne disease, which occurs and spreads in potato growing areas worldwide and poses a serious threat to potato production. New biofungicide is highly desirable for addressing the issue, and natural products (NPs) from Xenorhabdus spp. provide prolific resources for biofungicide development. In this study, we aim to identify antifungal NPs from Xenorhabdus spp. for the management of this disease.

Methods and results
Out of the 22 Xenorhabdus strains investigated, Xenorhabdus budapestensis 8 (XBD8) was determined to be the most promising candidate with the measured IC50 value of its cell-free supernatant against R. solani as low as 0.19 ml l−1. The major antifungal compound in XBD8 started to be synthesized in the middle logarithmic phase and reached a stable level at stationary phase. Core gene deletion coupled with high-resolution mass spectrometry analysis determined the major antifungal NPs as fabclavine derivatives, Fcl-7 and 8, which showed broad-spectrum bioactivity against important pathogenic fungi. Impressively, the identified fabclavine derivatives effectively controlled black scurf disease in both greenhouse and field experiments, significantly improving tuber quality and increasing with marketable tuber yield from 29 300 to 35 494 kg ha−1, comparable with chemical fungicide fludioxonil.

Conclusions
The fabclavine derivatives Fcl-7 and 8 were determined as the major antifungal NPs in XBD8, which demonstrated a bright prospect for the management of black scurf disease.
Preventing Adverse Outcomes for Bereaved Youth: Indirect Effects From a Randomized Trial of the Family Bereavement Program on Fear of Abandonment, Grief, and Mental Health
Authors: O’Hara Karey L , Wolchik Sharlene A , Rhodes C Aubrey , Uhlman Rana N , Sandler Irwin N , Tein Jenn-Yun 
Published: September 2023

Abstract

Objectives
We investigated whether the self-system belief of fear of abandonment mediated the effects of intervention-induced change in 2 protective factors—positive parenting and adaptive coping—and one risk factor—stressful events—on youth mental health problems and maladaptive grief. This study extends prior research on fear of abandonment in youth who experience parental death by examining pathways through which a program reduced fear of abandonment and, in turn, affected subsequent pathways to child mental health problems in the context of a randomized experiment.

Methods
This is a secondary data analysis study. We used data from the 4-wave longitudinal 2-arm parallel randomized controlled trial of the Family Bereavement Program conducted between 1996 and 1999 in a large city in the Southwestern United States. The sample consisted of 244 offspring between 8 and 16 at the pretest. They were assessed again at posttest, 11-month follow-up, and 6-year follow-up. Offspring, caregivers, and teachers provided data.

Results
Mediation analyses indicated that intervention-induced reductions in stressful events were prospectively associated with a lower fear of abandonment. For girls, fear of abandonment was related to self-reported maladaptive grief and teacher-reported internalizing problems 6 years later.

Conclusions
This study extends prior research on the relation between intervention-induced changes in risk and protective factors and improvements in outcomes of bereaved youth. The findings support the reduction of stressful events as a key proximal target of prevention programs for bereaved children.
High prevalence of central nervous system cryptococcosis using a fingerprick whole-blood lateral flow assay in individuals with neurological symptoms and advanced HIV disease in a Brazilian emergency department
Authors: Oliveira Fernanda Gurgel , Nakagawa Jeanne Aiko de Souza , de Oliveira Jefersson Matheus Maia , Júnior Rodovaldo Moraes Lucas , Marcusso Rosa , Vidal José E 
Published: September 2023

AbstractTimely diagnosis is key in managing central nervous system (CNS) cryptococcosis in people living with HIV/AIDS (PLWHA). There are few data on implementing fingerprick whole-blood cryptococcal antigen (CrAg) lateral flow assay (LFA) as the first test for diagnosing CNS cryptococcosis. We evaluated the prevalence of CNS cryptococcosis and cryptococcal antigenemia using fingerprick whole-blood in a referral emergency department (ED) in São Paulo, Brazil. This was a prospective cohort study of consecutive adult PLWHA with advanced HIV disease and neurological symptoms. Fingerprick whole-blood CrAg LFA was performed at bedside. Seventy-four individuals were enrolled (median age = 40 years; males = 62%). Prevalence of CNS cryptococcosis was 17.6% (13/74); 95% confidence interval (CI), 9.4–30.0%, and prevalence of positive fingerprick whole-blood CrAg LFA was 25.7% (19/74); 95% CI, 15.5–40.1%. Among the six (8.1%) patients with positive fingerprick whole-blood CrAg LFA and negative CSF CrAg LFA, four (5.4%) had isolated asymptomatic cryptococcal antigenemia, one (1.3%) had symptomatic cryptococcal antigenemia, and one (1.3%) had cryptococcemia. Prevalence of CNS cryptococcosis and cryptococcal antigenemia using fingerprick whole-blood CrAg LFA was high. Point-of-care testing was important for diagnosing CNS cryptococcosis in an ED from a middle-income country.
Studying X-ray spectra from large-scale jets of FR II radio galaxies: application of shear particle acceleration
Authors: He Jia-Chun , Sun Xiao-Na , Wang Jie-Shuang , Rieger Frank M , Liu Ruo-Yu , Liang En-Wei 
Published: September 2023

ABSTRACT
Shear particle acceleration is a promising candidate for the origin of extended high-energy emission in extra-galactic jets. In this paper, we explore the applicability of a shear model to 24 X-ray knots in the large-scale jets of FR II radio galaxies and study the jet properties by modelling the multiwavelength spectral energy distributions (SEDs) in a leptonic framework including synchrotron and inverse Compton–CMB processes. In order to improve spectral modelling, we analyse Fermi-LAT data for five sources and reanalyse archival data of Chandra on 15 knots, exploring the radio to X-ray connection. We show that the X-ray SEDs of these knots can be satisfactorily modelled by synchrotron radiation from a second, shear-accelerated electron population reaching multi-TeV energies. The inferred flow speeds are compatible with large-scale jets being mildly relativistic. We explore two different shear flow profiles (i.e. linearly decreasing and power law) and find that the required spine speeds differ only slightly, supporting the notion that for higher flow speeds the variations in particle spectral indices are less dependent on the presumed velocity profile. The derived magnetic field strengths are in the range of a few to 10 µG and the required power in non-thermal particles is typically well below the Eddington constraint. Finally, the inferred parameters are used to constrain the potential of FR II jets as possible ultra-high-energy cosmic-ray accelerators.