bluuebunny/crossref_metadata_2025_split huggingface dataset:
Applications of long-read sequencing to Mendelian genetics
Authors: Mastrorosa Francesco Kumara , Miller Danny E. , Eichler Evan E. 
Published: June 2023

AbstractAdvances in clinical genetic testing, including the introduction of exome sequencing, have uncovered the molecular etiology for many rare and previously unsolved genetic disorders, yet more than half of individuals with a suspected genetic disorder remain unsolved after complete clinical evaluation. A precise genetic diagnosis may guide clinical treatment plans, allow families to make informed care decisions, and permit individuals to participate in N-of-1 trials; thus, there is high interest in developing new tools and techniques to increase the solve rate. Long-read sequencing (LRS) is a promising technology for both increasing the solve rate and decreasing the amount of time required to make a precise genetic diagnosis. Here, we summarize current LRS technologies, give examples of how they have been used to evaluate complex genetic variation and identify missing variants, and discuss future clinical applications of LRS. As costs continue to decrease, LRS will find additional utility in the clinical space fundamentally changing how pathological variants are discovered and eventually acting as a single-data source that can be interrogated multiple times for clinical service.
Radiomics analysis of contrast-enhanced computed tomography in predicting the International Neuroblastoma Pathology Classification in neuroblastoma
Authors: Wang Haoru , Xie Mingye , Chen Xin , Zhu Jin , Zhang Li , Ding Hao , Pan Zhengxia , He Ling 
Published: June 2023

Abstract
Purpose
To predict the International Neuroblastoma Pathology Classification (INPC) in neuroblastoma using a computed tomography (CT)-based radiomics approach.

Methods
We enrolled 297 patients with neuroblastoma retrospectively and divided them into a training group (n = 208) and a testing group (n = 89). To balance the classes in the training group, a Synthetic Minority Over-sampling Technique was applied. A logistic regression radiomics model based on the radiomics features after dimensionality reduction was then constructed and validated in both the training and testing groups. To evaluate the diagnostic performance of the radiomics model, the receiver operating characteristic curve and calibration curve were utilized. Moreover, the decision curve analysis to assess the net benefits of the radiomics model at different high-risk thresholds was employed.

Results
Seventeen radiomics features were used to construct radiomics model. In the training group, radiomics model achieved an area under the curve (AUC), accuracy, sensitivity, and specificity of 0.851 (95% confidence interval (CI) 0.805–0.897), 0.770, 0.694, and 0.847, respectively. In the testing group, radiomics model achieved an AUC, accuracy, sensitivity, and specificity of 0.816 (95% CI 0.725–0.906), 0.787, 0.793, and 0.778, respectively. The calibration curve indicated that the radiomics model was well fitted in both the training and testing groups (p > 0.05). Decision curve analysis further confirmed that the radiomics model performed well at different high-risk thresholds.

Conclusion
Radiomics analysis of contrast-enhanced CT demonstrates favorable diagnostic capabilities in distinguishing the INPC subgroups of neuroblastoma.

Graphical Abstract

Critical relevance statement
Radiomics features of contrast-enhanced CT images correlate with the International Neuroblastoma Pathology Classification (INPC) of neuroblastoma.
Design and Development of a Novel 3-D Printed External Fixation Device for Fracture Stabilization
Authors: Skelley Nathan Wm. 
Published: June 2023

Abstract
Background
An external fixator is an orthopaedic device used to stabilize long bone fractures after high energy trauma. These devices are external to the body and fixed to metal pins going into non-injured areas of bone. They serve a mechanical function to maintain length, prevent bending, and resist torque forces about the fracture area. The purpose of this manuscript is to describe a design and prototyping process creating a low-cost entirely 3-D printed external fixator for fracture stabilization of extremity fractures. The secondary objective of this manuscript is to facilitate future advancements, modifications, and innovations in this area of 3-D printing in medicine.

Methods
This manuscript describes the computer aided design process using desktop fused deposition modeling to create a 3-D printed external fixator system designed for fracture stabilization. The device was created using the orthopaedic goals for fracture stabilization with external fixation. However, special modifications and considerations had to be accounted for given the limitations of desktop fused deposition modeling and 3-D printing with plastic polymers.

Results
The presented device accomplishes the goals of creating a construct that can be attached to 5.0 mm metal pins, allows for modularity in placement orientations, and facilitates adjustable lengths for fracture care. Furthermore, the device provides length stability, prevention of bending, and resists torque forces. The device can be printed on a desktop 3-D printer using standard low-cost polylactic acid filament. The print time is less than two days and can be completed on one print bed platform.

Conclusions
The presented device is a potential alternative for fracture stabilization. The concept of a desktop 3-D printed external fixator design and method of production allows for numerous diverse applications. This includes assisting areas with remote or limited access to advanced medical care and large-scale natural disasters or global conflicts where large volumes of fractures exceed the local medical supply chain capabilities. The presented device creates a foundation for future devices and innovations in this fracture care space. Further research is needed on mechanical testing and clinical outcomes with this design and initiative in fracture care before clinical application.
Mycorrhizal inoculation under water stress conditions and its influence on the benefit of host microbe symbiosis of Terminalia arjuna species
Authors: Bhardwaj Atul Kumar , Chandra K. K. , Kumar Rajesh 
Published: June 2023

Abstract
Background
Entisol is a very poor, compact, and low-water-holding capacity soil. They are obstacles to the plant's root system's penetration and the availability of water, particularly in dry months. However, Arbuscular mycorrhizae fungi (AMF) is used for seedling growth and reduces water stress in the plant.

Results
In this experiment, the growth parameters and the physiological activities of the plant were changed for the well watering (WW), fractionated watering (FW), and stopped/no watering conditions of the T. arjunaseedling. This experiment demonstrated higher mycorrhizal dependency (24.90%) under the FW condition than that of the WW condition (18.58%). Also the root colonization was higher (67%) under FW plants compared to WW plants (53%) associated with AMF+ in T. arjuna seedling. Photosynthesis was found 24.27% more with FW than the WW condition. Experiment' shows posivitivecorrelation between the photosynthesis and interval of no watering for AMF− plants (r2 = 0.873 for AMF− (control) and comparatively very weak for plants with AMF+ (r2 = 0.259 for AMF+ plants).

Conclusions
The findings confirms the use of AMF in entisol soil to improve plant growth and biomass by reducing edaphic stress.
IVUS-guided versus OCT-guided PCI among patients presenting with acute coronary syndrome
Authors: Abdelmonaem Mostafa , Abushouk Abdelrahman , Reda Ahmed , Arafa Sherif , Aboul- Enein Hisham , Bendary Ahmed 
Published: June 2023

Abstract
Background
Intravascular imaging modalities such as intravascular ultrasound (IVUS) and, more recently, optical coherence tomography (OCT) improved the visualization of coronary anatomy and plaque pathology. We aimed to compare the procedural and short-term outcomes between IVUS-guided and OCT-guided percutaneous coronary interventions (PCIs) in patients with acute coronary syndrome (ACS).

Methods
In the present retrospective study, we reviewed the data of 50 patients who had IVUS-guided PCI and 50 patients who had OCT-guided PCI for ACS between January 2020 and June 2021. Intravascular imaging was done before and after stenting. Both groups were compared in terms of minimal luminal area (MLA), stent dimensions, final minimal stent area (MSA) and stent expansion as well as negative angiographic outcomes. Patients were followed for six months to record major adverse cardiac events (MACE).

Results
The patients’ mean age was 57 ± 13 years with male predominance (78%). The radiation time and dose were significantly higher among IVUS group. Pre-stenting MLA was significantly higher in IVUS group (2.63 mm vs. 2.22 mm in OCT, P = 0.013). Stent expansion was significantly higher among OCT group (97% vs. 93% in IVUS group, P = 0.001) with no significant difference between both groups regarding MSA [mm2] (8.88 ± 2.87 in IVUS vs. 8.1 ± 2.76 in OCT, P = 0.169). No significant difference between both groups was noted regarding contrast volume, edge dissection, tissue prolapse, and no reflow. The rates of six-month MACE were significantly higher in the IVUS group.

Conclusions
OCT-guided PCI in ACS is safe and is associated with similar MSA to that of IVUS-guided PCI. Future randomized trials are needed to confirm these findings.

Graphical Abstract
Bridging the gap between clinical and critical sociological perspectives in dementia
Authors: Collins Noel , Fletcher James Rupert 
Published: June 2023

SUMMARYThere is a widening gap between the medical model of dementia and critical sociological perspectives of the condition. Given the relative failure of reductionism in dementia and its rising prevalence, consideration of the utility of these critical viewpoints is warranted. This article considers how these ideas, which challenge some prevailing assumptions about dementia, can be meaningfully applied in conjunction, rather than in competition, with conventional clinical ideas. To illustrate this, current perspectives on selfhood, biopolitics, citizenship and post-humanism are discussed. This article may also help to articulate sociologically oriented approaches already used by some clinicians and legitimise the time and attention needed to explore and deliver these. We support the view that dementia is an episteme in the making and that different traditions and dispositions can fruitfully collide to enliven interdisciplinary conversations about dementia and dementia care.
Consensus Statements on Precision Oncology in the China Greater Bay Area
Authors: Lam Tai-Chung , Cho William Chi-Shing , Au Joseph Siu-Kie , Ma Edmond Shiu-Kwan , Lam Stephen Tak-Sum , Loong Herbert Ho-Fung , Wong Jason Wing Hon , Wong S.N. Michael , Lee Victor Ho-Fun , Leung Roland Ching-Yu , Lau Johnny Kin-Sang , Kam Michael Tsz-Yeung , Mok Florence Siu-Ting , Lim Fiona Mei-Ying , Nyaw Jonathan Shi-Feng , Tin Winnie Wing-Yan , Cheung Ka-Man , Chan Oscar Siu-Hong , Kwong Philip Wai-Kay , Cheung Foon-Yiu , Poon Darren M.C. , Chik Jeannie Yin-Kwan , Lam Martin Ho-Ching , Chan Lawrence Wing-Chi , Wong Sze-Chuen Cesar , Cao Ya-Bing , Hui Cheng-Vai , Chen Jack Zhi-Jian , Chang Jian-Hua , Kong Spring Feng-Ming , El Helali Aya ,   on behalf of the Precision Oncology Working Group (POWG)
Published: June 2023

BACKGROUND Next-generation sequencing comprehensive genomic panels (NGS CGPs) have enabled the delivery of tailor-made therapeutic approaches to improve survival outcomes in patients with cancer. Within the China Greater Bay Area (GBA), territorial differences in clinical practices and health care systems and strengthening collaboration warrant a regional consensus to consolidate the development and integration of precision oncology (PO). Therefore, the Precision Oncology Working Group (POWG) formulated standardized principles for the clinical application of molecular profiling, interpretation of genomic alterations, and alignment of actionable mutations with sequence-directed therapy to deliver clinical services of excellence and evidence-based care to patients with cancer in the China GBA. METHODS Thirty experts used a modified Delphi method. The evidence extracted to support the statements was graded according to the GRADE system and reported according to the Revised Standards for Quality Improvement Reporting Excellence guidelines, version 2.0. RESULTS The POWG reached consensus in six key statements: harmonization of reporting and quality assurance of NGS; molecular tumor board and clinical decision support systems for PO; education and training; research and real-world data collection, patient engagement, regulations, and financial reimbursement of PO treatment strategies; and clinical recommendations and implementation of PO in clinical practice. CONCLUSION POWG consensus statements standardize the clinical application of NGS CGPs, streamline the interpretation of clinically significant genomic alterations, and align actionable mutations with sequence-directed therapies. The POWG consensus statements may harmonize the utility and delivery of PO in China's GBA.
The use of patient sitters at a Swiss hospital: A retrospective observational study
Authors: Kramer Iris , Schubert Maria 
Published: June 2023

Objective
Patient sitters are frequently used in acute care hospitals to provide one-to-one care for agitated or disorientated patients to assure the safety and well-being of patients. However, there is still a lack of evidence on the use of patient sitters, especially in Switzerland. Therefore, the aim of this study was to describe and explore the use of patient sitters in a Swiss acute care hospital.

Methods
In this retrospective, observational study we included all inpatients who were hospitalized between January and December 2018 in a Swiss acute care hospital and required a paid or volunteer patient sitter. Descriptive statistics were used to describe the extent of patient sitter use, patient characteristics, and organizational factors. For the subgroup analysis between internal medicine and surgical patients Mann-Whitney U tests and chi-square tests were used.

Results
Of the total of 27’855 included inpatients, 631 (2.3%) needed a patient sitter. Of these, 37.5% had a volunteer patient sitter. The median patient sitter duration per patient per stay was 18.0 hours (IQR = 8.4–41.0h). The median age was 78 years (IQR = 65.0–86.0); 76.2% of patients were over the age of 64. Delirium was diagnosed in 41% of patients, and 15% had dementia. Most of the patients showed signs of disorientation (87.3%), inappropriate behavior (84.6%), and risk of falling (86.6%). Patient sitter uses varied during the year and between surgical and internal medicine units.

Conclusions
These results add to the limited body of evidence concerning patient sitter use in hospitals, supporting previous findings related to patient sitter use for delirious or geriatric patients. New findings include the subgroup analysis of internal medicine and surgical patients, as well as analysis of patient sitter use distribution throughout the year. These findings may contribute to the development of guidelines and policies regarding patient sitter use.
Impact of maximal exercise on immune cell mobilization and bioenergetics
Authors: Stampley James E. , Cho Eunhan , Wang Haoyan , Theall Bailey , Johannsen Neil M. , Spielmann Guillaume , Irving Brian A. 
Published: June 2023

AbstractAcute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Eleven (7 M/4F) collegiate swimmers completed a maximal exercise test to measure anaerobic power and capacity. Pre‐ and postexercise PBMCs were isolated to measure the immune cell phenotypes and mitochondrial bioenergetics using flow cytometry and high‐resolution respirometry. The maximal exercise bout increased circulating levels of PBMCs, particularly in central memory (KLRG1+/CD57−) and senescent (KLRG1+/CD57+) CD8+ T cells, whether measured as a % of PMBCs or as absolute concentrations (all p < 0.05). At the cellularlevel, the routine oxygen flow (IO2 [pmol·s−1·106 PBMCs−1]) increased following maximal exercise (p = 0.042); however, there were no effects of exercise on the IO2 measured under the LEAK, oxidative phosphorylation (OXPHOS), or electron transfer (ET) capacities. There were exercise‐induced increases in the tissue‐level oxygen flow (IO2‐tissue [pmol·s−1·mL blood−1]) for all respiratory states (all p < 0.01), except for the LEAK state, after accounting for the mobilization of PBMCs. Future subtype‐specific studies are needed to characterize further maximal exercise's true impact on immune cell bioenergetics.
On double phase Kirchhoff problems with singular nonlinearity
Authors: Arora Rakesh , Fiscella Alessio , Mukherjee Tuhina , Winkert Patrick 
Published: June 2023

AbstractIn this paper, we study multiplicity results for double phase problems of Kirchhoff type with right-hand sides that include a parametric singular term and a nonlinear term of subcritical growth. Under very general assumptions on the data, we prove the existence of at least two weak solutions that have different energy sign. Our treatment is based on the fibering method in form of the Nehari manifold. We point out that we cover both the nondegenerate as well as the degenerate Kirchhoff case in our setting.
Necessary condition for temporary asteroid capture
Authors: Bao Changchun , Li Zhijie , Wang Xianyu 
Published: June 2023

Abstract
Asteroid capture holds great significance in understanding the origins of asteroids, the source of life, and the exploration of planetary mineral resources. To ensure the success of a mission aimed at capturing asteroids, it is crucial to choose asteroids that can be naturally captured by Earth. This article focuses on the orbital characteristics of temporarily captured asteroids. Through a statistical simulation of asteroid motion and an analytical derivation of the Jacobian integral, the necessary conditions for the temporary capture of asteroids by Earth are calculated. The analysis results show an approximately linear relationship between the orbit elements of temporarily captured asteroids. This condition is used to screen the currently observed near-Earth asteroids and has identified asteroids such as 2006 RH120 and 2020 CD3, which have been temporarily captured by Earth. These findings provide important suggestions for future asteroid capture missions and target selection.
Supersingular elliptic curves over ℤ𝑝-extensions
Authors: Çiperiani Mirela 
Published: June 2023

Abstract
Let

E
/
Q

\mathrm{E}/\mathbb{Q}

be an elliptic curve and 𝑝 a prime of supersingular reduction for

E

\mathrm{E}

.
Consider a quadratic extension

L
/

Q
p

L/\mathbb{Q}_{p}

and the corresponding anticyclotomic

Z
p

\mathbb{Z}_{p}

-extension

L
∞

/
L

L_{\infty}/L

.
We analyze the structure of the points

E
⁢

(

L
∞

)

\mathrm{E}(L_{\infty})

and describe two global implications of our results.
Compositional numerical analysis of multiphase flow of crude oil in porous media under non-isothermal conditions
Authors: Devarapu Srinivasa Reddy , Dau Tapas Kumar Dora , Kudapa Vamsi Krishna , Govindarajan Suresh Kumar 
Published: June 2023

Abstract
The present work details the development of a compositional model to replicate the heavy hydrocarbon flow in porous reservoir systems under non-isothermal conditions. The mathematical model considers mass and energy conservation equations describing the reactive of natural variables distributed in a multiphase hydrocarbon system. Such natural variable based compositional models better suit fully implicit numerical schemes with inexpensive Jacobian matrix computations. Further, the model accommodates a switch of primary variables for the disappearance and reappearance of a phase. The resulting nonlinear conservation equations are numerically discretized using a block-centered finite-difference scheme and solved with a quasi-Newton based implicit iterative solver. The present model is validated with the thermal profiles presented in the literature for the multiphase flow during the combustion of heavy crude oil in petroleum reservoir system with performance coefficient (R
2), mean absolute error (MBE), and maximum absolute percentage error (MAPE) of about 0.954, 0.37, and 0.01 respectively. The developed compositional model projected 26 and 72 % of light and heavy oil recoveries respectively in about 160 days with a maximum or peak temperature of about 798 K. Further, the thermal and production profiles projected by the sensitivity analysis on various operating parameters are presented. It is noteworthy that the present works aid in providing an economical numerical based tool in evaluating the flow and transport during underground or in-situ combustion process for efficient energy exploration.
Control of multi-agent systems: Results, open problems, and applications
Authors: Piccoli Benedetto 
Published: June 2023

Abstract
The purpose of this review article is to present some recent results on the modeling and control of large systems of agents. We focus on particular applications where the agents are capable of independent actions instead of simply reacting to external forces. In the literature, such agents were referred to as autonomous, intelligent, self-propelled, greedy, and others. The main applications we have in mind are social systems (as opinion dynamics), pedestrians’ movements (also called crowd dynamics), animal groups, and vehicular traffic. We note that the last three examples include physical constraints; however, the agents are able to inject energy into the system, thus preventing the typical conservation of momentum and energy. In addition, the control problems posed by such systems are new and require innovative methods. We illustrate some ideas developed recently, including the use of sparse controls, limiting the total variation of controls, and defining new control problems for measures. After reviewing various approaches, we discuss some future research directions of potential interest. The latter encompasses both new types of equations and new types of limiting procedures to connect several scales at which a system can be represented. We conclude by illustrating a recent real-life experiment using autonomous vehicles on an open highway to smooth traffic waves. This opens the door to a new era of interventions to control real-time multi-agent systems and to increase the societal impact of such interventions guided by control research.
Multi-material additive manufacturing: investigation of the combined use of ABS and PLA in the same structure
Authors: Yermurat Bagdaulyet , Seçgin Ömer , Taşdemir Vedat 
Published: June 2023

Abstract
3D printing using multi-materials has been one of the most popular topics recently. Fused deposition modeling (FDM) is one of the most widely used techniques for the three-dimensional printing of plastics and composites by all industries. In this study, acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA) were printed in the same structure with an independent double extruder FDM 3D printer. In the study, three different layer thicknesses (0.1 mm, 0.2 mm, and 0.3 mm), three different infill ratios (30%, 60%, and 90%) and three different infill types (hexagon, triangle, and 3D infill) were used. Tensile specimens were produced according to the ASTM D638 type-IV standard. Tensile specimens of 4 mm in thickness were produced in 1 mm ABS and PLA layers. As a result of the tensile tests, it has been seen that when PLA and ABS are used together in the same structure, the tensile strength increases significantly.