bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aluminum foil negative electrodes with multiphase microstructure for all-solid-state Li-ion batteries
Authors: Liu Yuhgene , Wang Congcheng , Yoon Sun Geun , Han Sang Yun , Lewis John A. , Prakash Dhruv , Klein Emily J. , Chen Timothy , Kang Dae Hoon , Majumdar Diptarka , Gopalaswamy Rajesh , McDowell Matthew T. 
Published: July 2023

AbstractMetal negative electrodes that alloy with lithium have high theoretical charge storage capacity and are ideal candidates for developing high-energy rechargeable batteries. However, such electrode materials show limited reversibility in Li-ion batteries with standard non-aqueous liquid electrolyte solutions. To circumvent this issue, here we report the use of non-pre-lithiated aluminum-foil-based negative electrodes with engineered microstructures in an all-solid-state Li-ion cell configuration. When a 30-μm-thick Al94.5In5.5negative electrode is combined with a Li6PS5Cl solid-state electrolyte and a LiNi0.6Mn0.2Co0.2O2-based positive electrode, lab-scale cells deliver hundreds of stable cycles with practically relevant areal capacities at high current densities (6.5 mA cm−2). We also demonstrate that the multiphase Al-In microstructure enables improved rate behavior and enhanced reversibility due to the distributed LiIn network within the aluminum matrix. These results demonstrate the possibility of improved all-solid-state batteries via metallurgical design of negative electrodes while simplifying manufacturing processes.
Next generation pan-cancer blood proteome profiling using proximity extension assay
Authors: Álvez María Bueno , Edfors Fredrik , von Feilitzen Kalle , Zwahlen Martin , Mardinoglu Adil , Edqvist Per-Henrik , Sjöblom Tobias , Lundin Emma , Rameika Natallia , Enblad Gunilla , Lindman Henrik , Höglund Martin , Hesselager Göran , Stålberg Karin , Enblad Malin , Simonson Oscar E. , Häggman Michael , Axelsson Tomas , Åberg Mikael , Nordlund Jessica , Zhong Wen , Karlsson Max , Gyllensten Ulf , Ponten Fredrik , Fagerberg Linn , Uhlén Mathias 
Published: July 2023

AbstractA comprehensive characterization of blood proteome profiles in cancer patients can contribute to a better understanding of the disease etiology, resulting in earlier diagnosis, risk stratification and better monitoring of the different cancer subtypes. Here, we describe the use of next generation protein profiling to explore the proteome signature in blood across patients representing many of the major cancer types. Plasma profiles of 1463 proteins from more than 1400 cancer patients are measured in minute amounts of blood collected at the time of diagnosis and before treatment. An open access Disease Blood Atlas resource allows the exploration of the individual protein profiles in blood collected from the individual cancer patients. We also present studies in which classification models based on machine learning have been used for the identification of a set of proteins associated with each of the analyzed cancers. The implication for cancer precision medicine of next generation plasma profiling is discussed.
3D printing of ultra-high viscosity resin by a linear scan-based vat photopolymerization system
Authors: Weng Zixiang , Huang Xianmei , Peng Shuqiang , Zheng Longhui , Wu Lixin 
Published: July 2023

AbstractThe current printing mechanism of the bottom-up vat photopolymerization 3D printing technique places a high demand on the fluidity of the UV-curable resin. Viscous high-performance acrylate oligomers are compounded with reactive diluents accordingly to prepare 3D printable UV-curable resins (up to 5000 cps of viscosity), yet original mechanical properties of the oligomers are sacrificed. In this work, an elaborated designed linear scan-based vat photopolymerization system is developed, allowing the adoption of printable UV-curable resins with high viscosity (> 600,000 cps). Briefly, this is realized by the employment of four rollers to create an isolated printing area on the resin tank, which enables the simultaneous curing of the resin and the detachment of cured part from the resin tank. To verify the applicability of this strategy, oligomer dominated UV-curable resin with great mechanical properties, but high viscosity is prepared and applied to the developed system. It is inspiring to find that high stress and strain elastomers and toughened materials could be facilely obtained. This developed vat photopolymerization system is expected to unblock the bottleneck of 3D printed material properties, and to build a better platform for researchers to prepare various materials with diversiform properties developed with 3D printing.
Automatically annotated motion tracking identifies a distinct social behavioral profile following chronic social defeat stress
Authors: Bordes Joeri , Miranda Lucas , Reinhardt Maya , Narayan Sowmya , Hartmann Jakob , Newman Emily L. , Brix Lea Maria , van Doeselaar Lotte , Engelhardt Clara , Dillmann Larissa , Mitra Shiladitya , Ressler Kerry J. , Pütz Benno , Agakov Felix , Müller-Myhsok Bertram , Schmidt Mathias V. 
Published: July 2023

AbstractSevere stress exposure increases the risk of stress-related disorders such as major depressive disorder (MDD). An essential characteristic of MDD is the impairment of social functioning and lack of social motivation. Chronic social defeat stress is an established animal model for MDD research, which induces a cascade of physiological and behavioral changes. Current markerless pose estimation tools allow for more complex and naturalistic behavioral tests. Here, we introduce the open-source tool DeepOF to investigate the individual and social behavioral profile in mice by providing supervised and unsupervised pipelines using DeepLabCut-annotated pose estimation data. Applying this tool to chronic social defeat in male mice, the DeepOF supervised and unsupervised pipelines detect a distinct stress-induced social behavioral pattern, which was particularly observed at the beginning of a novel social encounter and fades with time due to habituation. In addition, while the classical social avoidance task does identify the stress-induced social behavioral differences, both DeepOF behavioral pipelines provide a clearer and more detailed profile. Moreover, DeepOF aims to facilitate reproducibility and unification of behavioral classification by providing an open-source tool, which can advance the study of rodent individual and social behavior, thereby enabling biological insights and, for example, subsequent drug development for psychiatric disorders.
Cryo-EM structure and polymorphic maturation of a viral transduction enhancing amyloid fibril
Authors: Heerde Thomas , Schütz Desiree , Lin Yu-Jie , Münch Jan , Schmidt Matthias , Fändrich Marcus 
Published: July 2023

AbstractAmyloid fibrils have emerged as innovative tools to enhance the transduction efficiency of retroviral vectors in gene therapy strategies. In this study, we used cryo-electron microscopy to analyze the structure of a biotechnologically engineered peptide fibril that enhances retroviral infectivity. Our findings show that the peptide undergoes a time-dependent morphological maturation into polymorphic amyloid fibril structures. The fibrils consist of mated cross-β sheets that interact by the hydrophobic residues of the amphipathic fibril-forming peptide. The now available structural data help to explain the mechanism of retroviral infectivity enhancement, provide insights into the molecular plasticity of amyloid structures and illuminate the thermodynamic basis of their morphological maturation.
RUFY3 regulates endolysosomes perinuclear positioning, antigen presentation and migration in activated phagocytes
Authors: Char Rémy , Liu Zhuangzhuang , Jacqueline Cédric , Davieau Marion , Delgado Maria-Graciela , Soufflet Clara , Fallet Mathieu , Chasson Lionel , Chapuy Raphael , Camosseto Voahirana , Strock Eva , Rua Rejane , Almeida Catarina R. , Su Bing , Lennon-Duménil Ana-Maria , Nal Beatrice , Roquilly Antoine , Liang Yinming , Méresse Stéphane , Gatti Evelina , Pierre Philippe 
Published: July 2023

AbstractEndo-lysosomes transport along microtubules and clustering in the perinuclear area are two necessary steps for microbes to activate specialized phagocyte functions. We report that RUN and FYVE domain-containing protein 3 (RUFY3) exists as two alternative isoforms distinguishable by the presence of a C-terminal FYVE domain and by their affinity for phosphatidylinositol 3-phosphate on endosomal membranes. The FYVE domain-bearing isoform (iRUFY3) is preferentially expressed in primary immune cells and up-regulated upon activation by microbes and Interferons. iRUFY3 is necessary for ARL8b + /LAMP1+ endo-lysosomes positioning in the pericentriolar organelles cloud of LPS-activated macrophages. We show that iRUFY3 controls macrophages migration, MHC II presentation and responses to Interferon-γ, while being important for intracellular Salmonella replication. Specific inactivation of rufy3 in phagocytes leads to aggravated pathologies in mouse upon LPS injection or bacterial pneumonia. This study highlights the role of iRUFY3 in controlling endo-lysosomal dynamics, which contributes to phagocyte activation and immune response regulation.
Cognitive effects of Lewy body pathology in clinically unimpaired individuals
Authors: Palmqvist Sebastian , Rossi Marcello , Hall Sara , Quadalti Corinne , Mattsson-Carlgren Niklas , Dellavalle Sofia , Tideman Pontus , Pereira Joana B. , Nilsson Maria H. , Mammana Angela , Janelidze Shorena , Baiardi Simone , Stomrud Erik , Parchi Piero , Hansson Oskar 
Published: July 2023

Abstractα-Synuclein aggregates constitute the pathology of Lewy body (LB) disease. Little is known about the effects of LB pathology in preclinical (presymptomatic) individuals, either as isolated pathology or coexisting with Alzheimer’s disease (AD) pathology (β-amyloid (Aβ) and tau). We examined the effects of LB pathology using a cerebrospinal fluid α-synuclein-seed amplification assay in 1,182 cognitively and neurologically unimpaired participants from the BioFINDER study: 8% were LB positive, 26% Aβ positive (13% of those were LB positive) and 16% tau positive. LB positivity occurred more often in the presence of Aβ positivity but not tau positivity. LB pathology had independently negative effects on cross-sectional and longitudinal global cognition and memory and on longitudinal attention/executive function. Tau had cognitive effects of a similar magnitude, but these were less pronounced for Aβ. Participants with both LB and AD (Aβ and tau) pathology exhibited faster cognitive decline than those with only LB or AD pathology. LB, but not AD, pathology was associated with reduced sense of smell. Only LB-positive participants progressed to clinical LB disease over 10 years. These results are important for individualized prognosis, recruitment and choice of outcome measures in preclinical LB disease trials, but also for the design of early AD trials because >10% of individuals with preclinical AD have coexisting LB pathology.
Molluscicidal activity of Nicotiana tabacum extracts on the invasive snail Pomacea canaliculata
Authors: Guo Jing , Zhang Shaobin , Zeng Jian , Chen Yingtong , Guo Yongxin , Liu Jinling , He Ailan 
Published: July 2023

AbstractBotanical molluscicides for controlling the invasive snail Pomacea canaliculata have attracted worldwide attention because of their cost and environmental friendliness. Aqueous extracts from discarded tobacco leaf (Nicotiana tobacum) were evaluated for molluscicidal activity against different-sized P. canaliculata under laboratory conditions. The results showed that over 90% of the snails died in 1 g/L tobacco extract within 4 days, and the survival of P. canaliculata was inversely proportional to the snail size, tobacco extract concentration and length of exposure time. Adult males were more susceptible to tobacco extract than females. The snails had few chances to feed or mate in 0.5 g/L tobacco extract, and reproduction was greatly limited in 0.2 g/L. The growth of juvenile snails was inhibited in 0.2 g/L tobacco extract, but adults were unaffected. The antioxidant capacity of P. canaliculata in response to tobacco extract can be size- and sex-dependent, and the activities of superoxide dismutase, catalase, and acetylcholinesterase and the contents of glutathione and malondialdehyde were increased in adult males. These results suggest that discarded tobacco leaves can be useful as a molluscicide for controlling the invasive snail P. canaliculata based on its effects on survival, behaviour, food intake, growth performance and antioxidant capacity.
Diagnosis of genitourinary tuberculosis: detection of mycobacterial lipoarabinomannan and MPT-64 biomarkers within urine extracellular vesicles by nano-based immuno-PCR assay
Authors: Kamra Ekta , Prasad Tulika , Rais Anam , Dahiya Bhawna , Sheoran Abhishek , Soni Aishwarya , Sharma Suman , Mehta Promod K. 
Published: July 2023

AbstractWe detected a cocktail of Mycobacterium tuberculosis lipoarabinomannan (LAM) and MPT-64 biomarkers within urine extracellular vesicles (EVs) of genitourinary TB (GUTB) patients by nano-based immuno-PCR (I-PCR) assay, i.e., magnetic bead-coupled gold nanoparticle-based I-PCR (MB-AuNP-I-PCR) and compared the results with I-PCR and Magneto-ELISA. The size (s) of urine EVs ranged between 52.6 and 220.4 nm as analyzed by transmission electron microscopy (TEM) and nanoparticle tracking analysis. Functionalized AuNPs (coupled with detection antibodies/oligonucleotides) were characterized by UV–vis spectroscopy, TEM, ELISA, PCR, Atomic Force Microscopy and Fourier Transform Infrared spectroscopy, while conjugation of capture antibodies with MBs was validated by UV–vis spectroscopy and Magneto-ELISA. Our MB-AuNP-I-PCR exhibited sensitivities of 85% and 87.2% in clinically suspected (n = 40) and total (n = 47) GUTB cases, respectively, with 97.1% specificity in non-TB controls (n = 35). These results were further authenticated by the quantitative SYBR Green MB-AuNP-real-time I-PCR (MB-AuNP-RT-I-PCR). Concurrently, I-PCR and Magneto-ELISA showed sensitivities of 68.1% and 61.7%, respectively in total GUTB cases, which were significantly lower (p < 0.05–0.01) than MB-AuNP-I-PCR. Markedly, a wide range (400 fg/mL–11 ng/mL) of LAM+MPT-64 was quantified within urine EVs of GUTB cases by SYBR Green MB-AuNP-RT-I-PCR, which can assess the disease dynamics. This study will certainly improve the current algorithms used in GUTB diagnostics.
BCD spectrophotometry for massive stars in transition phases
Authors: Aidelman Y. J. , Borges Fernandes M. , Cidale L. S. , Smith Castelli A. , Arias M. L. , Zorec J. , Kraus M. , Torres A. , Souza T. B. , Cochetti Y. R. 
Published: July 2023

Context. Stars in transition phases, such as those showing the B[e] phenomenon and luminous blue variables (LBVs), undergo strong, often irregular mass-ejection events. The prediction of these phases in stellar evolution models is therefore extremely difficult, if not impossible. As a result, their effective temperatures, their luminosities, and even their true nature are not fully known.
Aims. A suitable procedure to derive the stellar parameters of these types of objects is to use the BCD spectrophotometric classification system, which is based on the analysis of the Balmer discontinuity (BD). The BCD parameters (λ1, D) have the advantage that they are independent of interstellar extinction and circumstellar contributions.
Methods. We obtained low-resolution spectra for a sample of 14 stars with the B[e] phenomenon and LBVs. Using the BCD classification system, we derived the stellar and physical parameters. The study was complemented with the information provided by the JHK colour-colour diagram.
Results. For each star under investigation, the BCD system gives a complete set of fundamental parameters and related quantities such as luminosity and distance. Among the 14 studied stars, we confirmed the classification of HK Ori, HD 323771, and HD 52721 as pre-main sequence HAeB[e] stars, AS 202 and HD 85567 as FS CMa-type stars, and HD 62623 as sgB[e]. We also classified Hen 3- 847, CD-245721, and HD 53367 as young B[e] stars or FS CMa-type candidates, and HD58647 as a slightly evolved B[e] star. In addition, Hen 3–1398 is an sgB[e] and MWC 877, CPD–59 2854, and LHA 120-S 65 are LBV candidates. The stellar parameters of the latter two LBVs are determined for the first time. We also used the size-luminosity relation to estimate the inner radius of the dust disc around the pre-main sequence and main sequence B[e] stars.
Conclusions. Our results emphasise that the BCD system is an important and highly valuable tool with which to derive the stellar parameters and physical properties of B-type stars in transition phases. This method can be combined with near-infrared (NIR) colour-colour diagrams to determine or confirm the evolutionary stage of emission-line stars with dust discs.
Modeling the formation and eruption of coronal structures by linking data-driven magnetofrictional and MHD simulations for AR 12673
Authors: Daei F. , Pomoell J. , Price D. J. , Kumari A. , Good S. , Kilpua E. K. J. 
Published: July 2023

Context. The data-driven and time-dependent modeling of coronal magnetic fields is crucial for understanding solar eruptions. These efforts are complicated by the challenges of finding a balance between physical realism and computing efficiency. One possible technique is to couple two modeling approaches.
Aims. Our aim here is to showcase our progress in using time-dependent magnetofrictional model (TMFM) results as input to dynamical magnetohydrodynamic (MHD) simulations. However, due to the different evolution processes in these two models, using TMFM snapshots in an MHD simulation is nontrivial. We address these issues, both physically and numerically, discuss the incompatibility of the TMFM output to serve as the initial condition in MHD simulations, and show our methods of mitigating this. The evolution of the flux systems and the cause of the eruption are investigated.
Methods. TMFM is a prevalent approach that has proven to be a very useful tool in the study of the formation of unstable structures in the solar corona. In particular, it is capable of incorporating observational data as initial and boundary conditions and requires shorter computational time compared to MHD simulations. To leverage the efficiency of data-driven TMFM and also to simulate eruptive events in the MHD framework, one can apply TMFM up to a certain time before the expected eruption(s) and then proceed with the simulation in the full or ideal MHD regime in order to more accurately capture the eruption process.
Results. We show the results of a benchmark test case with a linked TMFM and MHD simulation to study the evolution of NOAA active region 12673. A rise of a twisted flux bundle through the MHD simulation domain is observed, but we find that the rate of the rise and the altitude reached depends on the time of the TMFM snapshot that was used to initialize the MHD simulation and the helicity injected into the system. The analysis suggested that torus instability and slip-running reconnection could play an important role in the eruption.
Conclusions. The results show that the linkage of TMFM and zero-β MHD models can be successfully used to model the eruptive coronal magnetic fields.
The hunt for formamide in interstellar ices
Authors: Slavicinska K. , Rachid M. G. , Rocha W. R. M. , Chuang K.-J. , van Dishoeck E. F. , Linnartz H. 
Published: July 2023

Context.Although solid-state pathways are expected to dominate the formation mechanisms of many complex organic molecules (COMs), very few COMs have been securely identified in interstellar ices, in stark contrast with the many COM detections in the gas phase. The launch of theJames WebbSpace Telescope (JWST) and its increase in sensitivity and spectral resolution opens the possibility of identifying more COMs in ices, but additional laboratory data are necessary. Formamide (NH2CHO) is one such COM that is of great interstellar and prebiotic relevance where more laboratory data are needed in the hunt for its presence in interstellar ices.Aims.This work aims to characterize the mid-IR spectra of formamide in its pure form as well as in mixtures of the most abundant interstellar ices via laboratory simulation of such ices, as well as to demonstrate how these laboratory spectra can be used to search for formamide in ice observations.Methods.Mid-IR spectra (4000–500 cm−1/2.5–20 μm) of formamide, both in its pure form as well as in binary and tertiary mixtures with H2O, CO2, CO, NH3, CH3OH, H2O:CO2, H2O:NH3, CO:NH3, and CO:CH3OH, were collected at temperatures ranging from 15–212 K.Results.Apparent band strengths and positions of eight IR bands of pure amorphous and crystalline formamide at various temperatures are provided. Three of these bands are identified as potential formamide tracers in observational ice spectra: the overlapping C = O stretch and NH2scissor bands at 1700.3 and 1630.4 cm−1(5.881 and 6.133 μm), the CH bend at 1388.1 cm−1(7.204 μm), and the CN stretch at 1328.1 cm−1(7.529 μm). The relative apparent band strengths, positions, and full width half maxima (FWHM) of these features in mixtures at various temperatures were also determined. All of the laboratory spectra are available to the community on the Leiden Ice Database for Astrochemistry (LIDA) for use in the interpretation of both observations (e.g., from JWST) and laboratory spectroscopic data. Finally, the laboratory spectra are compared to observational spectra of a variety of low- and high-mass young stellar objects as well as prestellar cores observed with the Infrared Space Observatory, theSpitzerSpace Telescope, and JWST. A comparison between the formamide CH bend in laboratory data and the 7.24 μm band in the observations tentatively indicates that, if formamide ice is contributing significantly to the observed absorption, it is more likely in a polar matrix. Upper limits ranging from 0.35-5.1% with respect to H2O were calculated via scaling the formamide:H2O laboratory spectrum to the observations. These upper limits are in agreement with gas-phase formamide abundances and take into account the effect of a H2O matrix on formamide’s band strengths.
Correction of Sagittal Deformity with Bone Resection and Soft Tissue Releases
Authors: Stephens Joseph D. , Lombardi Adolph V. 
Published: July 2023

AbstractFixed flexion deformities (FFDs) present several unique challenges in total knee arthroplasty (TKA) and require careful consideration to achieve optimal outcomes. FFD alters normal knee biomechanics and increases energy expenditure. They may be attributed to both bony deformities and fibrosed soft tissues, which must be addressed in stepwise fashion at the time of surgery. A literature review was conducted utilizing keywords “fixed flexion deformity TKA.” This review article aims to discuss the anatomy and pathology, preoperative evaluation, classification system, surgical techniques for addressing deformity, implant selection considerations, postoperative protocols, clinical outcomes, and potential complications associated with correcting FFD. By comprehensively addressing these aspects, surgeons can optimize surgical planning and improve outcomes in patients with FFD undergoing TKA.