bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hsa_circ_0002371/hsa-miR-502-5p/ATG16L1 axis modulates the survival of intracellular Mycobacterium tuberculosis and autophagy in macrophages
Authors: Zhang Jinyi , He Yumo , Ruan Qiaoling , Bi Aixiao , Zhou Jingyu , Weng Shufeng , Ma Huixia , Lin Taiyue , Wang Honghai , Xu Ying 
Published: July 2023

Abstract

Background
Circular RNAs (circRNAs) play a critical role in pathological mechanisms of Mycobacterium tuberculosis (Mtb) and can be used as a new biomarker for tuberculosis (TB) diagnosis. Therefore, we identified significantly dysregulated circRNAs in TB patients and healthy controls (HC) and explored the role of hsa_circ_0002371 in TB and its molecular mechanism.

Methods

RNA sequencing was performed to identify significantly dysregulated circRNAs in TB patients and HC. The diagnostic value of hsa_circ_0002371 was evaluated by receiver operating characteristic (ROC) curve analysis. Real-time quantitative polymerase chain reaction (RT-qPCR), western blot (WB), immunofluorescence, and colony-forming unit (CFU) assay were conducted to investigate the role of hsa_circ_0002371in BCG-infected THP-1 human macrophages. Bioinformatics, fluorescence in situ hybridization (FISH), dual-luciferase reporter gene assay, and WB were used to explore the underlying molecular mechanisms.

Results

Hsa_circ_0002371 was significantly up-regulated in PBMCs of TB patients and H37Rv- or BCG-infected THP-1 human macrophages. Functional experiments demonstrated that hsa_circ_0002371 inhibited autophagy of BCG-infected THP-1 human macrophages and promoted intracellular BCG survival rate. Mechanistically, hsa_circ_0002371 promoted the expression of hsa-miR-502-5p, and hsa_circ_0002371overexpression-induced protective effects in BCG-infected THP-1 human macrophages was largely overturned by the inhibition of hsa-miR-502-5p. Notably, hsa-miR-502-5p inhibited autophagy via suppressing autophagy related 16 like 1 (ATG16L1) in BCG-infected macrophages and thus promoting intracellular BCG growth. In summation, hsa_circ_0002371 increased the suppression of hsa-miR-502-5p on ATG16L1 and inhibited autophagy to promote Mycobacteria growth in macrophages.
Conclusion
Our data suggested that hsa_circ_0002371 was significantly up-regulated in the PBMCs of TB patients compared with HC. The hsa_circ_0002371/hsa-miR-502-5p/ATG16L1 axis promoted the survival of intracellular Mtb and inhibited autophagy in macrophages. Our findings suggested hsa_circ_0002371 could act as a potential diagnostic biomarker and therapeutic target.
Dynamic tail modeling and motion analysis of a beaver-like robot
Authors: Chen Gang , Xu Yidong , Wang Zhenyu , Tu Jiajun , Hu Huosheng , Chen Chen , Xu Yuhang , Chai Xinxue , Zhang Jingjing , Shi Jianwei 
Published: July 2023

Abstract
The beaver-like underwater robot can crawl and swim, playing a significant role in the exploration of amphibious environments. Its fan-shaped tail can provide part of the forward power and maintain its motion stability. However, no mature dynamic modeling method is available to model its tail motion until now. This paper presents a segmented dynamic modeling approach to build the propulsion and lift models of a beaver-like robot tail. It combines hydrodynamics and material mechanics and forms the fan-shaped flexible dynamics modeling theory. A performance index is created to measure the motion performance of the tail, which provides a theoretical basis for describing and evaluating the tail function. Simulations and experiments are conducted to verify the tail dynamic model and the motion performance under different swing amplitude coefficients. The obtained optimal swing amplitude coefficient is 2, which lays the foundation for robot motion control. The study could further promote the development of dynamic modeling and analysis for other underwater biomimetic robots.
Structure and function of the geldanamycin amide synthase from Streptomyces hygroscopicus
Authors: Kirschning Andreas , Zeilinger Carsten , Preller Matthias , Ewert Wiebke , Bartens Christian , Ongouta Jekaterina , Holmes Monika , Heutling Anja , Kishore Anusha 
Published: July 2023

AbstractAmide synthases catalyze the formation of macrolactam rings from aniline-containing polyketide-derivedseco-acids as found in the important class of ansamycin antibiotics. One of these structurally and functionally hitherto undescribed amide synthases is the geldanamycin amide synthaseShGdmF, which we isolated for the first time and studied in detail both functionally as well as structurally. Here we show that purifiedShGdmF catalyzes the amide formation using synthetically derived simplified substrates. The atomic structures of the apo enzyme and in complex with simplified substrates reveal distinct structural features of the substrate binding site and a putative role of the flexible interdomain region for the catalysis reaction.
Nitrogen-contained nanoporous hyper-cross-linked polymer: A new turn-on fluorescence probe for detection of Ag+ ions in aqueous media
Authors: Nasimpoor Fariba , Mansournia Mohammadreza , Badiei Alireza 
Published: July 2023

Abstract
A fluorescence probe was designed using nitrogen-contained mesoporous hyper-cross-linked polymer precursor (NH2-HCP) in order to selectively detect silver (Ag+) ions. NH2-HCP has a fluorescence intensity, but only upon the addition of Ag+, a significant enhancement in fluorescence signal is observed, and the relationship between fluorescence intensity enhancement and Ag+ concentration exhibit a linear and monotonic trend. The probe's response to various other cations such as Al3+, Fe3+, Cd2+, Ni2+, Cu2+, Fe2+, Hg2+, Mg2+, Zn2+, Pb2+, Mn2+, Co2+, Ca2+, Na+, and K+ was also investigated. Under optimal conditions, the probe demonstrated a linear range of 0.1-3 μM and a detection limit of 0.01 μM.
Depressive symptoms and their associations with anger and aggression in Russian adolescents
Authors: Ruchkin Vladislav , Stickley Andrew , Koposov Roman , Sukhodolsky Denis G. , Isaksson Johan 
Published: July 2023

Abstract
This study investigated the prevalence of clinically significant (current major depressive episode) and subthreshold depressive symptoms and explored their association with aggression and anger, while controlling for comorbid mental health problems in a general population sample of adolescents from Northern Russia. The sample consisted of 2600 participants, aged 13–17 years (59.3% female; 95.7% ethnic Russian). Symptoms of a major depressive episode, types of anger and aggression (anger rumination, trait anger, physical, verbal and social aggression) and comorbid problems (posttraumatic stress, alcohol use, anxiety, and hyperactivity/impulsivity) were assessed by means of self-reports. The prevalence of a clinically significant depressive episode in the past month was 3.5%, while for subthreshold depression it was 21.6%. All anger and aggression variables, as well as comorbid problems increased together with increasing levels of depression. When adjusting for comorbidity, depression was only significantly associated with social aggression and anger rumination. Among adolescents with depression, boys reported higher levels of social and verbal aggression and of anger rumination than girls. Overt aggressive behavior associated with depression was primarily explained by comorbid mental health problems, whereas anger rumination and social aggression had more direct associations with depression, independent of comorbidity and were gender-specific. The results of this study suggest that interventions aiming to reduce aggressive behavior in adolescents should consider depression and its comorbid conditions.
Adaptive super-twisting global nonsingular terminal sliding mode control for robotic manipulators
Authors: Sun Chenchen , Huang Zhangmin , Wu Haiteng 
Published: July 2023

Abstract
This paper develops a novel global nonsingular terminal sliding mode control (GNTSMC) strategy based on an adaptive super-twisting algorithm (STA) for tracking control of robotic manipulators with uncertain perturbations. A novel global nonsingular terminal sliding manifold is designed to steer the system trajectory to reach the switching surface at the beginning, thereby removing the reaching stage and achieving strong robustness throughout the entire response. Moreover, the proposed sliding manifold can ensure the finite time convergence of the trajectory error to the origin. Then, an adaptive STA, which does not require the boundary information of the perturbations, is devised not only to attenuate the chattering effect without degrading the tracking precision, but also to guarantee the finite time stability of the system. Finally, the superiority of the adopted GNTSMC is validated by comparative studies.
Poor Quality of Sleep among Women during the perinatal period in Ethiopia: Systematic Review and Meta-analysis
Authors: Tinsae Techilo , Shumet Shegaye , segon Tesfaye , Melkam Mamaru , Medifu Girmaw , Rtbey Gidey , Nakie Girum , Fentahun Setegn , Andualem Fentahun , Getinet Wondale 
Published: July 2023

Abstract
Background
Sleep is a very crucial physiological process for human beings. During pregnancy and the postpartum period, sleep becomes very vital and it needs additional total sleep time for a better pregnancy outcome. However, poor sleep quality remains a major public health concern, particularly for perinatal women. Therefore, the pooled prevalence and risk factors from the study will provide a more conclusive result to take evidence-based measures against poor sleep in perinatal women.
Methods
Ten published studies with a total of 4,297 participants were included. All appropriate databases and grey literature were searched to get relevant articles. Studies reporting the prevalence and associated risk factors of poor sleep quality among perinatal women were included. The quality of each study was assessed using the Newcastle-Ottawa quality assessment Scale (NOS). Data were extracted using Microsoft Excel 2010 and the analysis was done using STATA version 11 software. The pooled prevalence and its associated factors were determined using the random effect model. Heterogeneity between studies was evaluated using the I2 test. In addition, Publication bias was checked in subjective technique by funnel plot and using Egger’s statistical test.
Results
The pooled prevalence of poor sleep quality was 44.81% (95% CI = 32.29, 57.34; I2 = 99.1%). Depression ((POR) = 3.87: 95% CI: 1.09, 12.40; I2 = 0.0%) and third-trimester ((POR) = 4.09: 95% CI: 1.05, 15.39; I2 = 0.0%) were risk factors of poor sleep quality.
Conclusion
More than two-thirds of perinatal women were exposed to poor quality of sleep. This indicates poor quality of sleep is a high health burden in women during the perinatal period in Ethiopia. The Government should incorporate maternal mental health policy along with prenatal and postnatal health care services.
Imaging Analysis of the Biological Parameters of the Lens in Patients with Cortical Age-Related Cataracts Using Ultrasound Biomicroscopy
Authors: Yu Zhiying , Li Na , Wang Fenglei , Fu Jing , Xue Shasha , Wang Ling 
Published: July 2023

Abstract
Background
The spatial position of the lens in patients with cortical age-related cataract (CARC) is unclear. We investigated a basis for the assessment of visual quality after cataract surgery by analysing the ultrasound biomicroscopic characteristics of the biological parameters of the lens in patients with (CARC)
Methods
In this retrospective study, 119 patients (50 males and 69 females, totalling 238 eyes) with CARC who underwent simple cataract surgery were selected. The lens thickness (LT), axial length (AL), anterior chamber depth (ACD), lens vault (LV), trabecular-iris angle (TIA), iris-lens angle (ILA), iris-lens contact distance (ILCD) were measured by A-scan ultrasound and ultrasound biomicroscopy. The corresponding lens position (LP) and relative lens position (RLP) were calculated.
Results
LP was greater in men than in women (P < 0.05), LV was smaller in men than in women (P = 0.002), ILA and ILCD were not statistically significant (P = 0.072 and P = 0.854, respectively). There were significant differences in TIA, ILA, and ILCD in the four quadrants (all P < 0.05), with a trend in the distribution of TIA: superior < inferior < nasal < temporal, ILA: nasal < inferior < temporal < superior, and ILCD: superior < temporal < inferior < nasal.
Conclusions
The lens protrudes more obviously in females than in males and the lens tilts to a certain extent with the increase of age and tends to be more upward and temporal in the supine position. Therefore, trends in lens-related parameters in patients with CARC should be taken seriously.
Efficacy, Safety, and Predictive Model of Palbociclib in the Treatment of HR-Positive and HER2-Negative Metastatic Breast Cancer
Authors: Wang Wei , Lei Wenqian , Fang Ziru , Jiang Ruiyuan , Wang Xiaojia 
Published: July 2023

Abstract

Purpose This research designeded to: 1. Analyze the efficacy and safety of Palbociclib treatment in HR-positive and HER2-negative (HR+/HER2-) metastatic breast cancer(MBC) patients. 2. Establish and validate a nomogram model for predicting the progression-free survival (PFS) rates of 6 months, 12 months, and 18 months in HR+/HER2- MBC patients after receiving Palbociclib plus endocrine therapy (ET). Patients and Methods: 1. This research retrospectively analyzed the efficacy and safety of Palbociclib combined with ET in 214 patients with HR+/HER2- MBC. 2. A nomogram was designed and constructed with the retrospective clinical data of 214 patients with HR+/HER2- MBC who received Palbociclib plus ET at Zhejiang Cancer Hospital in China from August 2018 to August 2022. Among these patients, 161 were randomly assigned to the training cohort, while 53 to the validation cohort. The predictive accuracy of the nomogram was assessed through the analysis the area under the receiver operating characteristic(ROC) curve, calibration curve, and decision curve analysis(DCA). Results 1. Median PFS was 7.17 months (95% CI: 7.61–10.05 months), with an objective response rate (ORR) of 2.80% and a disease control rate (DCR) of 34.58%. The most prevalent grade 3–4 adverse event was neutropenia (38.79%). 2. Multiple variable analysis of the training set revealed that age < 60 years old, PR < 20%, Ki-67 ≥ 20%, luminal B molecular subtype, primary resistance to ET, receipt of late-stage chemotherapy, and presence of liver metastasis or ≥ 2 visceral metastases were independent prognostic factors associated with poor PFS (P < 0.05). Then, the predictive model underwent development and validation utilizing the aforementioned parameters. On the one hand, the area under the ROC curve (AUC) values of the training set at 6 months, 12 months, and 18 months were 0.771, 0.783, and 0.790, respectively, indicating a strong predictive ability of the developed model. On the other hand, the AUC of the validation set at 6 months, 12 months, and 18 months were 0.720, 0.766, and 0.754, respectively, suggesting the favorable discriminatory ability of the model. The calibration curves also exhibited a good fit with the ideal curves, and the DCA demonstrated the clinical applicability of the model. The nomogram's different scores could distinguish PFS. Conclusion This retrospective study demonstrates the efficacy of Palbociclib in Chinese breast cancer patients. Moreover, the clinical parameters showed a significant association with the prognosis of HR+/HER2- MBC, and the prognostic models constructed based on these variables also displayed robust predictive power, which could offer more intuitive and convenient references for clinical doctors to formulate follow-up treatment plans.
Examination of Medicinal Plants for Radionuclides Absorption and Their Health Implications
Authors: Popoola Felix Adegoke , Isola Oladotun Bamiji , Fakeye Oluseye Daniel , Owolabi Tunde Ayobami , Sanyaolu Modupe Eunice , Owoyemi Sheu Isiaq , Elijah Isaac Oluwafemi 
Published: July 2023

Abstract
This study tests the concentration of 40K, 238U, and 232Th radionuclides and also evaluates the possible radiological health risks in medicinal plants found in Ewu, Edo State, Nigeria, using a NaI(Tl) gamma spectrometer. The six selected medicinal plants are Magnifera indica, Dacryodes edulis, Terminalia catappa, Cymbopogon citratus, Anacardium occidentale, and Persea Americana. The results showed that the activity concentrations for 40K ranged from 146.59 ± 4.81 in Persea americana to 296.08 3.42 Bq/kg in Cymbopogon citratus with a mean of 209.43 ± 5.14 Bq/kg, 238U ranged from 2.25 ± 0.06 to 5.57 ± 0.15 Bq/kg with a mean of 4.73 ± 0.15 Bq/kg and 232Th varied from 4.50 ± 0.35 to 12.07 ± 0.57 Bq/kg with a mean of 8.00 ± 0.40 Bq/kg. The maximum and minimum activity concentrations of both 238U and 232Th were found in Magnifera indica and Cymbopogon citratus respectively. The calculated radiological hazards assessment due to the investigated medicinal plants is well within the internationally recommended safe limits. 232Th contributes 54.91% to the total ECED , while 6.35% for 238U is the least. 232Th exhibit a very strong, positive, and significant relationship with (ECED and ELCR), and it contributes largely to the ECED and ELCR due to ingestion of the examined herbal plant. Therefore, the use of these medicinal plants is radiologically safe for human consumption.
A study to evaluate the extent of bone healing in mandibular distraction osteogenesis using preoperative and postoperative pixel value ratio
Authors: yuhe shen , Kong Liangliang , weimin shen , Chen Xinjian 
Published: July 2023

Abstract

Background
the aim of this study was to investigate the value and potential influencing factors of pixel value ratio (PVR) in early phase of mandible healing by distraction osteogenesis.
Methods
We performed a retrospective analysis on 342 patients with bilateral mandibular lengthening surgery, measured PVRs in osteotomy position of children before surgery, and monitored PVRs in early stage of bone regenerating at 1st, 2nd and 3rd month respectively after surgery. The potential effects of gender, weight, and age on fixation period were also analyzed.
Results
PVRs before osteotomy and at 1st, 2nd and 3rd month after osteotomy were (R0.96 ± 0.04, L0.97 ± 0.05), (R0.4 ± 0.1, L0.42 ± 0.1), (R0.68 ± 0.11, L0.71 ± 0.11), (R0.97 ± 0.04, L0.98 ± 0.04) which had significant differences (p < 0.001). We found no significant difference in PVRs by gender. In terms of age, there was a significant difference in PVRs between children aged less than 28 days and older than 120 days before surgery. There were also significant differences between those aged less than 28 days and older than 120 days, and children aged 8–28 days and aged 29–60 days at the 3rd month after surgery. Consider weight, children with birth weight less than 4 kg and those with admission weight less than 2.5 kg were significant different.
Conclusion
PVRs were gradually increased during the early 3 months after the osteotomy. Gender had no effect on PVRs, while age, birth weight, and admission weight had a greater effect. Our results may inform the assessment of PVRs and potential impact of patients with early bone lengthening.
Characteristics and Risk Factors of Severe infection in ANCA-associated vasculitis
Authors: liang xiaowan , wang tingting , zhu xiaoye , mao xiaoyi , wu yuanhao , lu lin , you li , Xue Jun 
Published: July 2023

Abstract
Background: There is a close relationship between ANCA-associated vasculitis and infections. Based on a cohort of ANCA-associated vasculitis patients from East China, this study aimed todescribe the infection characteristics and prognosis of the patients, and explore the risk factors for severe infections.
Methods: Relevant data from the cohort of ANCA-associated vasculitispatients (n=82) at the affiliated Huashan Hospital, Fudan University, collected between 2012 and 2021 was retrospectively reviewed to describe the basic characteristics and treatment response of the completely relieved vasculitis patients (n=63) and non-completely relieved AAV patients (n=19), and to compare the incidence of severe infections and prognosis between AAV patients with underlying infections (n=17) and those without infection(n=65).
Results: 65% of the infections occurred within the first three months of AAV-induced treatment. The main site of common infection was the urinary system, with Escherichia coli being the most common pathogen. The main site of severe infections was the respiratory system, and the main pathogenic bacteria included Klebsiella pneumoniae, Candida albicans, and Aspergillus. The complete remission rate of patients with underlying infections before diagnosis of AAV was lower than that of patients without underlying infections before the onset of the disease (58.82% vs 81.84%, p=0.048), and even if the dose of immunosuppressants was reduced, the incidence of severe infections in patients with underlying infections was higher than that in patients without infections (63.64% vs 32.36%, p=0.0055). The presence of underlying infections at the time of diagnosis of AAV (HR=4.569, 95% CI=1.518-14.302, p=0.007), elevated ALT at the onset of the disease (HR=3.163, 95% CI=1.060-9.371, p=0.039), and the use of cyclophosphamide (HR=4.081, 95% CI=1.246-13.279, p=0.020) were found to be the risk factors for severe infections.
Conclusions: Bacterial and fungal pneumonia are the main types of severe infection after ANCA-associated vasculitis immunosuppressive therapy. The presence of underlying infection and impaired liver and kidney function at the time of diagnosis of AAV are independent risk factors for severe infections in ANCA-associated vasculitis patients after receiving immunosuppressive therapy. Patients with underlying infection with AAV have a lower complete response rate and are still prone to severe infection after receiving reduced immunosuppressive therapy.
Long-term continuous cultivation of Kenyan infant fecal microbiota using the host adapted PolyFermS model
Authors: Rachmühl Carole , Lacroix Christophe , Cabrera Paula Momo , Geirnaert Annelies 
Published: July 2023

Abstract
Appropriate in vitro models to investigate the impact of novel nutritional strategies on the gut microbiota of infants living in rural Africa are scarce. Here, we aimed to develop such a continuous gut fermentation model based on the PolyFermS platform. Eight immobilized Kenyan infant fecal microbiota were used as inoculum for continuous PolyFermS colon models fed with medium mimicking the weaning infant diet. Fructo-oligosaccharides (FOS) supplementation (1, 4 and 8 g/L) and cultivation pH (5.8 and 6.3) were stepwise investigated. Conditions providing a close match between fecal and in vitro microbiota (pH 5.8 with 1 g/L FOS) were selected for investigating long-term stability of four Kenyan infant PolyFermS microbiota. The shared fraction of top bacterial genera between fecal and in vitro microbiota was high (74–89%) and stable during 107 days of continuous cultivation. Community diversity was maintained, and two distinct fermentation metabolite profiles, propiogenic and butyrogenic, of infant fecal microbiota established from day 8 onwards and stayed stable. We present here the first rationally designed and accurate continuous cultivation model of African infant gut microbiota. This model will be important to assess the effect of dietary or environmental factors on the gut microbiota of African infants with high enteropathogen exposure.