bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antinutrient removal in yellow lentils by malting
Authors: Cimini Alessio , Poliziani Alessandro , Morgante Lorenzo , Moresi Mauro 
Published: August 2023

AbstractBACKGROUNDTo improve the current low per capita consumption of lentils, the present study first aimed to minimize the anti‐nutrient content of two yellow Moroccan and Italian lentil seeds by resorting to the malting process and then testing the resulting decorticated flours as ingredients in the formulation of gluten‐free fresh egg pastas.RESULTSThe most proper operating conditions for the three malting process steps were identified in a bench‐top plant. The first (water steeping) and second (germination) steps were studied at 18, 25 or 32 °C. After 2 or 3 h of steeping at 25 °C and almost 24 h of germination, 95–98.8% of the lentil seeds sprouted. By prolonging the germination process to 72 h, the raffinose or phytic acid content was reduced by about 80% or 95% or 27% or 37%, respectively. The third step (kilning) was carried out under fluent dry air at 50 °C for 24 h and at 75 °C for 3 h. The cotyledons of the resulting yellow lentil malts were cyclonically recovered, milled and chemico‐physically characterized.CONCLUSIONBoth flours were used to prepare fresh egg‐pastas essentially devoid of oligosaccharides, and low in phytate (4.6–6.0 mg g−1) and in vitro glycemic index (38–41%). However, the cooking quality of the fresh egg pasta made of malted Moroccan lentil flour was higher with respect to its crude protein content and lower with respect to its water solubility index. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
μ‐PESI‐based MS profiling combined with untargeted metabolomics analysis for rapid identification of red wine geographical origin
Authors: Pu Keyuan , Wang Yue , Wei Huiwen , Hu Jun , Qiu Jiamin , Chen Siyu , Liu Qian , Lin Yan , Ng Kwan‐Ming 
Published: August 2023

AbstractBACKGROUNDThe commercial value of red wine is strongly linked to its geographical origin. Given the large global market, there is great demand for high‐throughput screening methods to authenticate the geographical source of red wine. However, only limited techniques have been established up to now.RESULTSHerein, a sensitive and robust method, namely probe electrospray ionization mass spectrometry (μ‐PESI‐MS), was established to achieve rapid analysis at approximately 1.2 min per sample without any pretreatment. A scotch near the needle tip provides a fixed micro‐volume for each analysis to achieve satisfactory ion signal reproducibility (RSD < 26.7%). In combination with a machine learning algorithm, 16 characteristic ions were discovered from thousands of detected ions and were utilized for differentiating red wine origin. Among them, the relative abundances of two characteristic metabolites (trigonelline and proline) correlated with geographical conditions (sun exposure and water stress) were identified, providing the rationale for differentiation of the geographical origin.CONCLUSIONThe proposed μ‐PESI‐MS‐based method demonstrates a promising high‐throughput determination capability in red wine traceability.
In vitro study of biogenic amine production and gastrointestinal stress tolerance by some enterococci strains
Authors: Houicher Abderrahmane , Kuley Esmeray , Bensid Abdelkader , Yazgan Hatice , Özogul Fatih 
Published: August 2023

AbstractBACKGROUNDOwing to the toxic effects of enterococci, their biogenic amine production is a negative aspect of safe strain selection and unfavourable activity in food. Additionally, the ability to tolerate acid and bile are two important traits for the selection of probiotic strains since they show the probiotic bacteria's capacity to survive throughout gastrointestinal transit. In the present work, six enterococci strains belonging to Enterococcus gallinarum and Enterococcus hirae were subjected to in vitro evaluation of their ability to produce biogenic amines and to tolerate gastrointestinal stress in order to investigate their possibility for future intended use as probiotics.RESULTSAll enterococci isolates possessed good viability at low pH (pH 4) and in the presence of bile salts (0.3%), indicating their ability to survive passage through the gastrointestinal tract. In addition, selected strains had a high ability to produce tyramine in tyrosine decarboxylase broth, while medium levels of histamine were detected (below 74 mg L−1) in experimental media in vitro. Other biogenic amines were also formed at various levels by most of the enterococci strains.CONCLUSIONAll enterococci strains, with the exception of E. gallinarum DM 29, are powerful tyramine producers, and their capacity to create histamine is inferior to that of tyramine. However, more investigations are needed before considering their use as bio‐preservatives or starter cultures in foods. © 2023 Society of Chemical Industry.
Global patterns and controls of soil greenhouse gas fluxes and crop yield under biochar application
Authors: Jia Xiaoyu , Yan Weiming , Yang Jingyi , Chen Weiguang , Ma Hongze , Chen Xi , Liu Jin , Zhong Yangquanwei , Shangguan Zhouping 
Published: August 2023

AbstractBiochar application has been proven to be a viable technique in mitigating soil greenhouse gas (GHG) emissions and improving crop yield; however, the global patterns and responses of soil GHG fluxes, global warming potential (GWP), crop yield, and greenhouse gas intensity (GHGI) to biochar application, as well as the key drivers of biochar‐induced changes, are not fully synthesized. Hence, we conducted a meta‐analysis of 3883 paired observations from 220 studies. Overall, biochar application significantly increased carbon dioxide (CO2) emissions (7.4%) and yield (9.4%) and decreased soil methane (CH4, −13.0%) and nitrous oxide (N2O, −26.8%) emissions, as well as GWP (−7.7%) and GHGI (−14.3%). CH4 uptake was, however, not observed to change. The responses of soil GHG fluxes to the biochar application were more sensitive in the incubation and pot experiments compared to the field studies. In addition, increased CO2 emissions were mainly derived from incubation and pot experiments, whereas the biochar application did not significantly change CO2 emissions in the field studies. The climate, soil, and experimental conditions also affected the responses of soil GHG fluxes to biochar application, and the biochar‐induced change in soil pH was the most essential factor associated with the variations in soil GHG fluxes. Globally, biochar application boosted the soil carbon (C) pool (38.1% in upland and 33.4% in paddy) and decreased soil GHGI (−18.7% in upland and −14.5% in paddy). Our study highlights the role of the reduced CH4 and N2O emissions in the biochar‐induced mitigation of soil GHG emissions while exploring the relative importance of the influencing factors and providing crucial insights for the development of individualized biochar management measures.
Chaperone‐mediated autophagy: Molecular mechanisms, biological functions, and diseases
Authors: Yao Ruchen , Shen Jun 
Published: August 2023

AbstractChaperone‐mediated autophagy (CMA) is a lysosomal degradation pathway that eliminates substrate proteins through heat‐shock cognate protein 70 recognition and lysosome‐associated membrane protein type 2A‐assisted translocation. It is distinct from macroautophagy and microautophagy. In recent years, the regulatory mechanisms of CMA have been gradually enriched, including the newly discovered NRF2 and p38–TFEB signaling, as positive and negative regulatory pathways of CMA, respectively. Normal CMA activity is involved in the regulation of metabolism, aging, immunity, cell cycle, and other physiological processes, while CMA dysfunction may be involved in the occurrence of neurodegenerative disorders, tumors, intestinal disorders, atherosclerosis, and so on, which provides potential targets for the treatment and prediction of related diseases. This article describes the general process of CMA and its role in physiological activities and summarizes the connection between CMA and macroautophagy. In addition, human diseases that concern the dysfunction or protective role of CMA are discussed. Our review deepens the understanding of the mechanisms and physiological functions of CMA and provides a summary of past CMA research and a vision of future directions.
Thymol targeting interleukin 4 induced 1 expression reshapes the immune microenvironment to sensitize the immunotherapy in lung adenocarcinoma
Authors: Li Tong , Shi Jie , Wang Longlong , Qin Xuan , Zhou Rui , Dong Ming , Ren Fan , Li Xin , Zhang Zihe , Chen Yanan , Liu Yanhua , Piao Yongjun , Shi Yi , Xu Song , Chen Jun , Li Jia 
Published: August 2023

AbstractImmune checkpoint blockades are the most promising therapy in lung adenocarcinoma (LUAD). However, the response rate remains limited, underscoring the urgent need for effective sensitizers. Interleukin 4 induced 1 (IL4I1) is reported to have immunoinhibitory and tumor‐promoting effects in several cancers. However, the targetable value of IL4I1 in sensitizing the immunotherapy is not clear, and there is a lack of effective small molecules that specifically target IL4I1. Here, we show that silencing IL4I1 significantly remodels the immune microenvironment via inhibiting aryl hydrocarbon receptor (AHR) signaling, thereby enhancing the efficacy of anti‐PD‐1 antibody in LUAD, which suggests that IL4I1 is a potential drug target for the combination immunotherapy. We then identify thymol as the first small molecule targeting IL4I1 transcription through a drug screening. Thymol inhibits the IL4I1 expression and blocks AHR signaling in LUAD cells. Thymol treatment restores the antitumor immune response and suppresses the progression of LUAD in an orthotopic mouse model. Strikingly, the combination treatment of thymol with anti‐PD‐1 antibody shows significant tumor regression in LUAD mice. Thus, we demonstrate that thymol is an effective small molecule to sensitize the PD‐1 blockade in LUAD via targeting IL4I1, which provides a novel strategy for the immunotherapy of LUAD.
Thoracic dumbbell giant schwannoma in a young woman: A case report
Authors: Jun Zheng , Bing Yu , Qing Song Li 
Published: August 2023

AbstractSchwannomas, which are benign, are one of the most common intraspinal tumors, accounting for 29% of primary spinal tumors. Schwannomas have several other names, including neurinoma, neurilemmoma, and neuroma. They originate from Schwann cells, which were first described by Theodor Schwann, a German physiologist, biologist, and histologist. Here, we present a patient with a giant, thoracic, dumbbell‐shaped schwannoma. This benign tumor protruded into the subcutaneous fascial layer, compressing the pleura and the left side of the thoracic spinal cord. Additionally, some of the spinous processes and laminae of the thoracic vertebrae were missing. Most patients with this type of tumor are asymptomatic; however, up to 11.7% of patients have symptoms that depend on the size and location of the tumor. Magnetic resonance imaging can accurately determine the extent of the tumor and any intraspinal component and is thus helpful in selecting the optimal surgical procedure.
Newer antidepressant for Japanese adults with major depressive disorder: A systematic review and meta‐analysis
Authors: Kishi Taro , Sakuma Kenji , Hatano Masakazu , Matsuda Yuki , Esumi Satoru , Miyake Nobumi , Miura Itaru , Hori Hikaru , Kato Masaki , Iwata Nakao 
Published: August 2023

AbstractIntroductionThe question remains to be elucidated: “Is treatment with antidepressants at doses approved in Japan effective for Japanese patients with MDD?” It is crucial to confirm this in order to provide appropriate treatments for Japanese patients with major depressive disorder (MDD). Therefore, we conducted a systematic review and random‐effects pairwise meta‐analysis including these nine double‐blind, randomized, placebo‐controlled trials.MethodsWe calculated the standardized mean difference (SMD) and risk ratio (RR) with a 95% confidence interval (95% CI).ResultsPooled newer antidepressants outperformed placebo regarding improvement of depressive symptom scale scores [SMD (95% CI) = −0.20 (−0.27, −0.12), p < 0.00001], response to treatment [RR (95% CI) = 1.23 (1.13, 1.32), p < 0.00001], and remission rate [RR (95% CI) = 1.30 (1.16, 1.45), p < 0.00001]. Although all‐cause discontinuation was not significantly different between the treatment groups, the pooled antidepressant group showed a higher discontinuation rate due to adverse event [RR (95% CI) = 1.60 (1.13, 2.26), p = 0.007] and a higher incidence of at least one adverse event than the placebo group [RR (95% CI) = 1.13 (1.08, 1.18), p < 0.00001].DiscussionWe concluded that newer antidepressants are effective for Japanese adults with MDD although the clinicians must monitor the health conditions of these individuals.
Enhancing the foaming properties of polypropylene/basalt fiber composites by altering their rheological and crystallization behavior via polyethylene–octene blending modification
Authors: Zhu Nenggui , Shen Chao , Zeng Xiangbu , Li Shengnan , Jiang Tuanhui , Gong Wei , He Li 
Published: August 2023

AbstractThe quality of foam in polypropylene/basalt fiber (PP/BF) composite materials is currently unsatisfactory, which restricts their use in industrial applications. To address this limitation, the objective of this study is to improve the foaming properties of PP/BF composite foam by modifying their rheological and crystallization behavior using polyethylene–octene (POE) blending modification. The results demonstrate that the inclusion of POE has a beneficial impact on the rheological and crystallization characteristics of PP/BF composites. Consequently, this creates favorable conditions for the foaming process of PP/BF composite foam. It is conducive to obtaining high foaming quality PP/BF composite foam. The results demonstrate that a 5 wt% POE content yields the best foaming performance for the PP/BF composite foam, with the most uniform cell distribution, a cell size of 54 μm and a cell density is measured at 3.43 × 107 cells/cm3. The optimal temperature range for foaming is between 180 and 200°C. The introduction of POE in PP/BF composite foam may lead to a decrease in its tensile and flexural strength. However, this drawback is offset by a significant improvement in the notch impact strength of the foam. This study's findings will enhance our understanding of the production and application of PP/BF composite foam, making it a valuable material for applications that require exceptional toughness.Highlights
Improved the crystallization and rheological properties of PP/BF composites.
Significantly improve the foaming quality of PP/BF composites.
Make PP/BF composite foam has excellent impact toughness.
A novel epoxy adhesive with ground rubber tire powder and carboxylated multiwalled carbon nanotubes
Authors: Kazemi Esfeh Tannaz , Arefinia Reza , Moini Jazani Omid , Fallahi Mohsen 
Published: August 2023

AbstractThe present study was an attempt to improve the toughness, mechanical and adhesion properties, and thermal stability of a two‐phase epoxy DGEBA/Resole phenolic resin through the use of carboxyl‐modified multi‐walled carbon nanotubes (c‐MWCNTs) and ground rubber tire (GRT) powder. The effect of a hybrid reinforcement containing GRT and c‐MWCNT blend on the mechanical properties, thermal stability, and microstructure of the epoxy‐phenolic adhesive were evaluated by tensile test, TGA, and FESEM, respectively. The formulated adhesive was utilized in a lap joint metal to a composite in order to peruse the adhesion features, where the composite was an epoxy resin reinforced with carbon fibers and the metal was stainless steel 316 L. According to the tensile test, there were 32% rise in tensile strength, 27% rise in modulus, and a 64% rise in toughness with adding 0.5% wt c‐MWCNTs and 15 phr waste GRT powder to the epoxy‐phenolic matrix. Furthermore, adding 1% wt c‐MWCNTs of nanoparticles and GRT powder has resulted in a 15% increase in lap shear strength. It also can be seen that thermal stability for the formulated samples has experienced an upward trend compared to the neat epoxy composite.Highlights
Epoxy adhesives based on GRT Powder, c‐MWCNTs particles and phenolic resin have been successfully prepared.
The hybrid uses of GRT Powder and c‐MWCNTs particles can synergistically increase the tensile and lap shear strength
The addition of c‐MWCNT nano particles and GRT Powder in an epoxy adhesive can have a dramatic rise in the toughness system compared to the neat epoxy adhesive.
Maximum degradation temperature of the produced newly adhesive has increased considerably in contrast with the neat epoxy adhesive.
The importance of the continuity of practice: Ethnobotany of Kihnu island (Estonia) from 1937 to 2021
Authors: Sõukand Renata , Kalle Raivo , Prakofjewa Julia , Sartori Matteo , Pieroni Andrea 
Published: August 2023

Societal Impact StatementLocal knowledge is highly endangered in the modern world, and therefore, it is important to understand the factors contributing to the preservation of biocultural diversity. Three major aspects were identified: continuity of the practice, support for the ritual related to the use, and external acknowledgment of the local use by an authoritative source. Moreover, strong centralization of current local knowledge in institutionalized domains (ethnomedicine) was identified, which has excluded unacknowledged or officially unapproved local knowledge from circulation. The inclusion of local ecological knowledge as a practice in school curricula and the highlighting of local historical uses in herbals and popularizing activities are recommended.Summary

Plant use in local communities changes over time along with changing social, political, economic, and environmental conditions. The study aimed to understand the factors influencing the continuity of certain interactions between plants and people.
To understand the drivers of resilience of the use of local flora, the historical (1930s) and current (2021) uses of plants on the small island of Kihnu in Estonia were compared, and the resilient uses were identified.
Use resilience depended on the use domain. While the ethnoveterinary domain completely disappeared, the most resilient uses were those related to ritual (religious) activities, with 75% being retained (6 of 8 taxa used historically). This was followed by the wild food plant domain, in which 66% of taxa (21 of 32) have been retained, along with the highest proportion of taxa‐use combinations (57%, 21 of 37). Historically the largest domain, ethnomedicine showed low resilience: 18 of 73 taxa have been retained, with only eight emic (or local) plant uses (PU). Moreover, we observed that 75% of the retained emic PU (6 out of 8) were supported by a centralized medical system, while this proportion was 87% for current uses and only 15% for interrupted uses that were promoted or acknowledged in centralized herbals.
As the most important aspects influencing the resilience of plant use are the continuity of practice, ritualization, and external support for usage, the inclusion of local ecological knowledge as a practice in school curricula and the highlighting of local historical uses in herbals and popularizing activities are strongly recommended.
Dynamic computed tomography for evaluation of tracheobronchomalacia in premature infants with bronchopulmonary dysplasia
Authors: Pugh Charles Preston , Ali Sumera , Agarwal Amit , Matlock David N. , Sharma Megha 
Published: August 2023

AbstractIntroductionDynamic computed tomography (dCT) gives real‐time physiological information and objective descriptions of airway narrowing in tracheobronchomalacia (TBM). There is a paucity of literature in the evaluation of TBM by dCT in premature infants with bronchopulmonary dysplasia (BPD). The aim of this study is to describe the findings of dCT and resultant changes in management in premature infants with TBM.MethodsA retrospective study of 70 infants was performed. Infants included were <32 weeks gestation without major anomalies. TBM was defined as ≥50% expiratory reduction in cross‐sectional area with severity defined as mild (50%–75%), moderate (≥75%–90%), or severe (≥90%).ResultsDynamic CT diagnosed malacia in 53% of infants. Tracheomalacia was identified in 49% of infants with severity as 76% mild, 18% moderate, and 6% severe. Bronchomalacia was identified in 43% of infants with varying severity (53% mild, 40% moderate, 7% severe). Resultant management changes included PEEP titration (44%), initiation of bethanechol (23%), planned tracheostomy (20%), extubation trial (13%), and inhaled ipratropium bromide (7%).ConclusionDynamic CT is a useful noninvasive diagnostic tool for airway evaluation of premature infants. Presence and severity of TBM can provide actionable information to guide more precise clinical decision making.
Molecular binding of different classes of organophosphates to methyl parathion hydrolase from Ochrobactrum species
Authors: Bhat Nayana , Nutho Bodee , Hanpaibool Chonnikan , Hadsadee Sarinya , Vangnai Alisa , Rungrotmongkol Thanyada 
Published: August 2023

AbstractMethyl parathion hydrolase (MPH) is an enzyme of the metallo‐β‐lactamase superfamily, which hydrolyses a wide range of organophosphates (OPs). Recently, MPH has attracted attention as a promising enzymatic bioremediator. The crystal structure of MPH enzyme shows a dimeric form, with each subunit containing a binuclear metal ion center. MPH also demonstrates metal ion‐dependent selectivity patterns. The origins of these patterns remain unclear but are linked to open questions about the more general role of metal ions in functional evolution and divergence within enzyme superfamilies. We aimed to investigate and compare the binding of different OP pesticides to MPH with cobalt(II) metal ions. In this study, MPH was modeled from Ochrobactrum sp. with different OP pesticides bound, including methyl paraoxon and dichlorvos and profenofos. The docked structures for each substrate optimized by DFT calculation were selected and subjected to atomistic molecular dynamics simulations for 500 ns. It was found that alpha metal ions did not coordinate with all the pesticides. Rather, the pesticides coordinated with less buried beta metal ions. It was also observed that the coordination of beta metal ions was perturbed to accommodate the pesticides. The binding free energy calculations and structure‐based pharmacophore model revealed that all the three substrates could bind well at the active site. However, profenofos exhibit a stronger binding affinity to MPH in comparison to the other two substrates. Therefore, our findings provide molecular insight on the binding of different OP pesticides which could help us design the enzyme for OP pesticides degradation.