bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENINGKATAN KEMAMPUAN TENTANG MANFAAT TANAMAN OBAT KELUARGA (TOGA) DI LINGKUNGAN MAJELIS TAKLIM MUSLIMATUL HIDAYAH, PONDOK CABE ILIR, TANGERANG SELATAN
Authors: Diarsi Eka Yani  , Huda Nurul , Farida Idha , Yuliana Ernik 
Published: March 2023

Salah satu model pengobatan yang terus berkembang dan diminati saat ini adalah dengan pengobatan yang menggunakan bahan-bahan alami yang sering dikenal dengan pengobatan herbal yang berbahan dasar tanaman obat keluarga (TOGA). Tanaman ini selain mempunyai banyak fungsi terutama bagi kesehatan, juga dapat dibudidayakan di lahan pekarangan yang sempit dan tidak membutuhkan banyak perawatan. Kegiatan abdimas yang terkait dengan TOGA ini dilakukan di Majelis Taklim Muslimatul Hidayah, Pondok Cabe Ilir, Tangerang Selatan. Tahapan kegiatan abdimas yaitu (1) survei lokasi/perijinan, (2) pelatihan secara teori tentang jenis, manfaat, pemberian materi tentang beberapa minuman berbahan dasar TOGA, (3) penyebaran leaflet untuk anggota majelis taklim, (4) pengukuran aspek pengetahuan dengan pengisian kuesioner, dan (5) monitoring serta evaluasi. Setelah mereka memahami tentang pengertian, tujuan, jenis, dan manfaat TOGA serta cara membuat minuman berbahan dasar TOGA, maka mereka dapat mempraktikkan pembuatan minuman berbahan dasar TOGA untuk merawat kesehatan tubuh masing-masing.
A PLATEIA DA CENA TEATRAL: OBJETIVOS PEDAGÓGICOS
Authors: Rosseto Robson 
Published: March 2023

Este texto abrange os objetivos pedagógicos do ensino do teatro em relação à recepçãoteatral e suas implicações. A recepção teatral foi examinada por duas perspectivas distintas: quanto í necessidade de instrumentar o educando para o melhor aproveitamento da apreciação teatral e quanto í necessidade de o professor estar qualificado para avaliar a observação/percepção de seus alunosenquanto espectadores.PALAVRAS-CHAVE: ensino do teatro, recepção, espectador.
Characterization of polar surface groups on siliceous materials by inverse gas chromatography and the enthalpy–entropy compensation effect
Authors: Meyer Ralf , Mueller Kai , Naumov Sergej , Bauer Frank , Enke Dirk 
Published: March 2023

Surface-modified porous silica is a well-established composite material. To improve its embedding and application behavior, adsorption studies of various probe molecules have been performed using the technique of inverse gas chromatography (IGC). For this purpose, IGC experiments were carried out in the infinite dilution mode on macro-porous micro glass spheres before and after surface modification with (3-mercaptopropyl)trimethoxysilane. To provide information about the polar interactions between probe molecules and the silica surface, in particular, eleven polar molecules have been injected. In summary, the free surface energy for pristine silica (γStotal = 229 mJ/m2) and for (3-mercaptopropyl)trimethoxysilane-modified silica (γStotal = 135 mJ/m2) indicates a reduced wettability after surface modification. This is due to the reduction of the polar component of the free surface energy (γSSP) from 191 mJ/m2 to 105 mJ/m2. Simultaneously, with the reduction of surface silanol groups caused by surface modification of silica and, therefore, the decrease in polar interactions, a substantial loss of Lewis acidity was observed by various IGC approaches. Experiments with all silica materials have been conducted at temperatures in the range from 90°C to 120°C to determine the thermodynamic parameters, such as adsorption enthalpy (ΔHads) and adsorption entropy (ΔSads), using the Arrhenius regression procedure evaluating the IGC data. With the help of the enthalpy–entropy compensation, two types of adsorption complexes are assumed between polar probe molecules and the silica surface because of different isokinetic temperatures. Identical adsorption complexes with an isokinetic temperature of 370°C have been assigned to alkanes and weakly interacting polar probes such as benzene, toluene, dichloromethane, and chloroform. Polar probe molecules with typical functional groups such as OH, CO, and CN, having the ability to form hydrogen bonds to the silica surface, exhibit a lower isokinetic temperature of 60°C. Quantum chemical calculations of the probe molecules on a non-hydroxylated and hydroxylated silica cluster supported the formation of hydrogen bonds in the case of a strong polar adsorption complex with a bonding distance of 1.7 nm–1.9 nm to the silica surface.
Bordetella spp. utilize the type 3 secretion system to manipulate the VIP/VPAC2 signaling and promote colonization and persistence of the three classical Bordetella in the lower respiratory tract
Authors: First Nicholas J. , Pedreira-Lopez Jose , San-Silvestre Manuel R. F. , Parrish Katelyn M. , Lu Xiao-Hong , Gestal Monica C. 
Published: March 2023

IntroductionBordetella are respiratory pathogens comprised of three classical Bordetella species: B. pertussis, B. parapertussis, and B. bronchiseptica. With recent surges in Bordetella spp. cases and antibiotics becoming less effective to combat infectious diseases, there is an imperative need for novel antimicrobial therapies. Our goal is to investigate the possible targets of host immunomodulatory mechanisms that can be exploited to promote clearance of Bordetella spp. infections. Vasoactive intestinal peptide (VIP) is a neuropeptide that promotes Th2 anti-inflammatory responses through VPAC1 and VPAC2 receptor binding and activation of downstream signaling cascades.MethodsWe used classical growth in vitro assays to evaluate the effects of VIP on Bordetella spp. growth and survival. Using the three classical Bordetella spp. in combination with different mouse strains we were able to evaluate the role of VIP/VPAC2 signaling in the infectious dose 50 and infection dynamics. Finally using the B. bronchiseptica murine model we determine the suitability of VPAC2 antagonists as possible therapy for Bordetella spp. infections.ResultsUnder the hypothesis that inhibition of VIP/VPAC2 signaling would promote clearance, we found that VPAC2-/- mice, lacking a functional VIP/VPAC2 axis, hinder the ability of the bacteria to colonize the lungs, resulting in decreased bacterial burden by all three classical Bordetella species. Moreover, treatment with VPAC2 antagonists decrease lung pathology, suggesting its potential use to prevent lung damage and dysfunction caused by infection. Our results indicate that the ability of Bordetella spp. to manipulate VIP/VPAC signaling pathway appears to be mediated by the type 3 secretion system (T3SS), suggesting that this might serve as a therapeutical target for other gram-negative bacteria.ConclusionTaken together, our findings uncover a novel mechanism of bacteria-host crosstalk that could provide a target for the future treatment for whooping cough as well as other infectious diseases caused primarily by persistent mucosal infections.
Body mass index affecting ticagrelor monotherapy vs. ticagrelor with aspirin in patients with acute coronary syndrome: A pre-specified sub-analysis of the TICO randomized trial
Authors: Kim Byung Gyu , Hong Sung-Jin , Kim Byeong-Keuk , Lee Yong-Joon , Lee Seung-Jun , Ahn Chul-Min , Shin Dong-Ho , Kim Jung-Sun , Ko Young-Guk , Choi Donghoon , Hong Myeong-Ki , Jang Yangsoo 
Published: March 2023

BackgroundAlthough ticagrelor monotherapy after 3-month dual antiplatelet therapy (DAPT) results in a significantly greater net clinical benefit over that with ticagrelor-based 12-month DAPT in patients with acute coronary syndrome (ACS), it remains uncertain whether this effect is dependent on body mass index (BMI). We aimed to evaluate the BMI-dependent effect of these treatment strategies on clinical outcomes.MethodsThis was a pre-specified subgroup analysis from the TICO trial (Ticagrelor Monotherapy After 3 Months in Patients Treated With New Generation Sirolimus-eluting Stent for Acute Coronary Syndrome), evaluating the interaction between BMI and treatment strategies for the primary outcome [composite of major bleeding and adverse cardiac and cerebrovascular events (MACCE): death, myocardial infarction, stent thrombosis, stroke, or target-vessel revascularization]. The secondary outcomes were major bleeding and MACCE.ResultsBased on a pre-specified BMI threshold of 25 kg/m2, 3,056 patients were stratified. Patients with BMI &lt;25 kg/m2 had a higher risk of primary and secondary outcomes than those with BMI ≥25 kg/m2. Regardless of the BMI subgroup, the effects of ticagrelor monotherapy after 3-month DAPT on the primary outcome (pint = 0.61), major bleeding (pint = 0.76), and MACCE (pint = 0.80) were consistent without significant interaction compared with ticagrelor-based 12-month DAPT. The treatment effects according to the BMI quartiles and age, sex, and diabetic status were also consistent without significant interaction.ConclusionThe BMI-dependent impact of ticagrelor monotherapy after 3-month DAPT compared with 12-month DAPT on clinical outcomes was not heterogeneous in patients with ACS.Clinical Trial Registration[www.ClinicalTrials.gov], identifier [NCT02494895].
Uric acid/superoxide dismutase can predict progression of gestational hypertension to preeclampsia
Authors: Yun Lin , Yu Xiaoqian , Xu Rui 
Published: March 2023

IntroductionPreeclampsia (PE), at early onset, is likely to be diagnosed as gestational hypertension (GH). Some cases of GH rapidly progress to PE within a short period of time, increasing the mortality rate of pregnant women and adverse events in neonates during the peripartum period. Oxidative stress participates in the occurrence and progression of PE. However, it is unknown whether the progression of GH to PE can be predicted.MethodsA total of 1548 patients diagnosed with PE (649 cases) or GH (899 cases) from January 2016 to June 2022 were selected as the study subjects. The 1548 patients were randomly divided into the training set (1083 cases) and the validation set (465 cases) in a 7:3 ratio. General and clinical data were collected to construct a risk factor prediction model for PE.ResultsWe found that (1) Systolic blood pressure (SBP), and uric acid (UA)/ superoxide dismutase (SOD) were the risk factors for the progression of GH to PE; (2) A nomogram was constructed from the prediction model, and the area under the curve (AUC) was 0.95, with a sensitivity of 87.4%, a specificity of 92.8%; (3) Build a model simplified scoring system. PE was most strongly predicted by UA/SOD (100 points), SBP (29 points), and serum potassium (19 points). The AUC was 0.92, with a sensitivity of 91.0%, a specificity of 81.7%. The clinical decision analysis curve shows that the model exhibits positive benefits when the threshold probability is at 0.01–0.91.ConclusionThese findings show that UA/SOD can predict progression of GH to PE.
Annual and semi-annual variations of electron density in the topside ionosphere observed by CSES
Authors: Zhu Keying , Yan Rui , Xiong Chao , Zheng Lin , Zhima Zeren , Shen Xuhui , Liu Dapeng , Guan Yibing , Liu Chao , Xu Song , Lv Fangxian , Guo Feng , Zhou Na 
Published: March 2023

In this paper, based on the observations from Langmuir probe (LAP) onboard the China Seismo-Electromagnetic Satellite (CSES), the annual/semi-annual variations of electron density (Ne) measured at 02:00 and 14:00 local time (LT) in the topside ionosphere have been analyzed. Results indicated that the Ne exhibits an amplification-linear-saturation with the increase of P10.7 in the daytime, while roughly linear in the nighttime. The annual/semi-annual variations of CSES Ne at around 500 km are found by morphological analysis and Morlet wavelet analysis, with dominant period of 187 days and 374 days. The annual components of longitude-averaged Ne dominate at most magnetic latitudes (Mlats) with maxima around the June solstices in the northern hemisphere and the December solstice in the southern hemisphere (except for the northern hemisphere in the nighttime), while the semi-annual variation dominates at the magnetic equator and low magnetic latitudes with two maxima at equinoxes. The Ne dominant period is characterized by a transition from semi-annual variation at the equator and low magnetic latitudes regions to annual variation at the middle magnetic latitudes region. The annual/semi-annual variations of Ne observed by CSES satellite show a consistent performance with previous studies, and have complemented the ionospheric characteristics at 500 km altitude, especially in the nighttime.
A novel DEAH-box helicase 37 mutation associated with differences of sex development
Authors: Wan Yun , Yu Richeng , Luo Jianhua , Huang Ping , Zheng Xingju , Sun Liqun , Hu Kui 
Published: March 2023

ObjectiveTo determine the genetic etiology of a family pedigree with two patients affected by differences of sex development (DSD).MethodsAssess the clinical characteristics of the patients and achieve exome sequencing results and in vitro functional studies.ResultsThe 15-year-old proband, raised as female, presented with delayed puberty and short stature associated with atypical genitalia. Hormonal profile showed hypergonadotrophic hypogonadism. Imaging studies revealed the absence of a uterus and ovaries. The karyotype confirmed a 46, XY pattern. Her younger brother presented with a micropenis and hypoplastic scrotum with non-palpable testis and hypospadias. Laparoscopic exploration was performed on the younger brother. Streak gonads were found and removed due to the risk of neoplastic transformation. Post-operative histopathology showed the co-existence of Wolffian and Müllerian derivatives. Whole-exome sequencing identified a novel mutation (c.1223C&gt;T, p. Ser408Leu) in the Asp-Glu-Ala-His-box helicase 37 gene, which was found to be deleterious by in silico analysis. Segregation analysis of the variant displayed a sex-limited, autosomal dominant, maternal inheritance pattern. In vitro experiments revealed that the substitution of 408Ser by Leu caused decreased DHX37 expression both at the mRNA and protein levels. Moreover, the β-catenin protein was upregulated, and the p53 protein was unaltered by mutant DHX37.ConclusionsWe described a novel mutation (c.1223C&gt;T, p. Ser408Leu) of the DHX37 gene associated with a Chinese pedigree consisting of two 46, XY DSD patients. We speculated that the underlying molecular mechanism might involve upregulation of the β-catenin protein.
Resolving trabecular metaphyseal bone profiles downstream of the growth plate adds value to bone histomorphometry in mouse models
Authors: Salmon P. L. , Monzem S. , Javaheri B. , Oste L. , Kerckhofs G. , Pitsillides A. A. 
Published: March 2023

IntroductionHistomorphometry of rodent metaphyseal trabecular bone, by histology or microCT, is generally restricted to the mature secondary spongiosa, excluding the primary spongiosa nearest the growth plate by imposing an ‘offset’. This analyses the bulk static properties of a defined segment of secondary spongiosa, usually regardless of proximity to the growth plate. Here we assess the value of trabecular morphometry that is spatially resolved according to the distance ‘downstream’ of—and thus time since formation at—the growth plate. Pursuant to this, we also investigate the validity of including mixed primary–secondary spongiosal trabecular bone, extending the analysed volume ‘upstream’ by reducing the offset. Both the addition of spatiotemporal resolution and the extension of the analysed volume have potential to enhance the sensitivity of detection of trabecular changes and to resolve changes occurring at different times and locations.MethodTwo experimental mouse studies of trabecular bone are used as examples of different factors influencing metaphyseal trabecular bone: (1) ovariectomy (OVX) and pharmacological prevention of osteopenia and (2) limb disuse induced by sciatic neurectomy (SN). In a third study into offset rescaling, we also examine the relationship between age, tibia length, and primary spongiosal thickness.ResultsBone changes induced by either OVX or SN that were early or weak and marginal were more pronounced in the mixed primary–secondary upstream spongiosal region than in the downstream secondary spongiosa. A spatially resolved evaluation of the entire trabecular region found that significant differences between experimental and control bones remained undiminished either right up to or to within 100 μm from the growth plate. Intriguingly, our data revealed a remarkably linear downstream profile for fractal dimension in trabecular bone, arguing for an underlying homogeneity of the (re)modelling process throughout the entire metaphysis and against strict anatomical categorization into primary and secondary spongiosal regions. Finally, we find that a correlation between tibia length and primary spongiosal depth is well conserved except in very early and late life.ConclusionsThese data indicate that the spatially resolved analysis of metaphyseal trabecular bone at different distances from the growth plate and/or times since formation adds a valuable dimension to histomorphometric analysis. They also question any rationale for rejecting primary spongiosal bone, in principle, from metaphyseal trabecular morphometry.
Current status and prospects of diabetes mellitus induced erectile dysfunction: A bibliometric and visualization study
Authors: Zhang Lei , Bao Binghao , Guo Jianqiang , Qin Zhongjian , Huang Haonan , Chen Lu , Liu Baoxing 
Published: March 2023

BackgroundThe prevalence of diabetes mellitus-induced erectile dysfunction (DMED) has recently increased, which has prompted numerous DMED studies. Here, we conduct a bibliometric analysis of relevant literature in the field of DMED and to discuss the research hotspots and future development directions.MethodsThe Web of Science Core Collection database was searched for literature on DMED, and literature characterization including the number of articles, journals, countries/regions, institutions, authors, keywords, and other information was performed using VOS viewer and CiteSpace software. In addition, Pajek software was used for visual map adjustment, and GraphPad Prism was used to generate line graphs.ResultsA total of 804 articles concerning DMED were included in this study. The Journal of Sexual Medicine issued the most documents(92 articles). The United States and China were in the leading position in the field of DMED research, and cross-institutional collaboration on DMED research worldwide needs to be further strengthened. Ryu JK were the authors with the highest number of documents issued (22 articles) while Bivalacqua TJ was the author with the most co-citated(249 co-citated). The keywords analysis shows that the main research hotspots in the field of DMED were mechanism discussions and disease treatment and management.ConclusionsGlobal research on DMED is expected to increase further. The investigation of the mechanism of DMED and the exploration of new therapeutic means and targets are the focus of future research.
The impact of freezing on the post-mortem human microbiome
Authors: Ogbanga Nengi , Nelson Andrew , Gino Sarah , Wescott Daniel J. , Mickleburgh Hayley L. , Gocha Timothy P. , Procopio Noemi 
Published: March 2023

IntroductionHuman donations are often used in forensic research as they can provide unique insights into post-mortem research that cannot be obtained with animal proxies. This is especially true for forensic microbiome research, as human circumstances such as drug-use or health conditions may influence the post-mortem microbiome. However, it is not always feasible to conduct such research immediately after death. Donors are often stored frozen in human taphonomy facilities, pending the start of any experimental study, yet little is known about how freezing may affect their microbiome.MethodsWe assessed the effects of freezing on the post-mortem human microbiome by analysing the microbial diversity and abundance of seven human donors at the Forensic Anthropology Center at Texas State (FACTS) before and after freezing. Swab samples were taken from five locations on each corpse upon arrival to FACTS and again after they had been frozen in storage for a period ranging between 11 and 40 days and subsequently thawed.ResultsAfter performing the microbiome analysis of the swabs via 16S rRNA gene metabarcoding, we found changes in the abundance levels of Proteobacteria, Bacteroidota and Firmicutes, as well as the presence of the new phyla Deinococcota and Myxococcota after freezing. However, none of these changes were significant when comparing community diversity before and after freezing.DiscussionOverall, our results show that the observed changes in the abundance of specific phyla before and after freezing are negligible, that freezing does not significantly alter the human microbiome and that frozen donors are suitable for forensic studies on the human thanatomicrobiome.
Prognostic value of autophagy-related genes based on single-cell RNA-sequencing in colorectal cancer
Authors: Luo Yuqi , Deng Xuesong , Liao Weihua , Huang Yiwen , Lu Caijie 
Published: March 2023

Background: Colorectal cancer (CRC) is the second most common cancer in China. Autophagy plays an important role in the initiation and development of CRC. Here, we assessed the prognostic value and potential functions of autophagy-related genes (ARGs) using integrated analysis using single-cell RNA sequencing (scRNA-seq) data from the Gene Expression Omnibus (GEO) and RNA sequencing (RNA-seq) data from The Cancer Genome Atlas (TCGA).Methods: We analyzed GEO-scRNA-seq data from GEO using various single-cell technologies, including cell clustering, and identification of differentially expressed genes (DEGs) in different cell types. Additionally, we performed gene set variation analysis (GSVA). The differentially expressed ARGs among different cell types and those between CRC and normal tissues were identified using TCGA-RNA-seq data, and the hub ARGs were screened. Finally, a prognostic model based on the hub ARGs was constructed and validated, and patients with CRC in TCGA datasets were divided into high- and low-risk groups based on their risk-score, and immune cells infiltration and drug sensitivity analyses between the two groups were performed.Results: We obtained single-cell expression profiles of 16,270 cells, and clustered them into seven types of cells. GSVA revealed that the DEGs among the seven types of cells were enriched in many signaling pathways associated with cancer development. We screened 55 differentially expressed ARGs, and identified 11 hub ARGs. Our prognostic model revealed that the 11 hub ARGs including CTSB, ITGA6, and S100A8, had a good predictive ability. Moreover, the immune cell infiltrations in CRC tissues were different between the two groups, and the hub ARGs were significantly correlated with the enrichment of immune cell infiltration. The drug sensitivity analysis revealed that the patients in the two risk groups had difference in their response to anti-cancer drugs.Conclusion: We developed a novel prognostic 11-hub ARG risk model, and these hubs may act as potential therapeutic targets for CRC.