bluuebunny/crossref_metadata_2025_split huggingface dataset:
A natural promoter variation of SlBBX31 confers enhanced cold tolerance during tomato domestication
Authors: Zhu Yingfang , Zhu Guangtao , Xu Rui , Jiao Zhixin , Yang Junwei , Lin Tao , Wang Zhen , Huang Sanwen , Chong Leelyn , Zhu Jian‐Kang 
Published: January 2023

SummaryCold stress affects crop growth and productivity worldwide. Understanding the genetic basis of cold tolerance in germplasms is critical for crop improvement. Plants can coordinate environmental stimuli of light and temperature to regulate cold tolerance. However, it remains unknown which gene in germplasms could have such function. Here, we utilized genome‐wide association study (GWAS) to investigate the cold tolerance of wild and cultivated tomato accessions and discovered that increased cold tolerance is accompanied with tomato domestication. We further identified a 27‐bp InDel in the promoter of the CONSTANS‐like transcription factor (TF) SlBBX31 is significantly linked with cold tolerance. Coincidentally, a key regulator of light signalling, SlHY5, can directly bind to the SlBBX31 promoter to activate SlBBX31 transcription while the 27‐bp InDel can prevent S1HY5 from transactivating SlBBX31. Parallel to these findings, we observed that the loss of function of SlBBX31 results in impaired tomato cold tolerance. SlBBX31 can also modulate the cold‐induced expression of several ERF TFs including CBF2 and DREBs. Therefore, our study has uncovered that SlBBX31 is possibly selected during tomato domestication for cold tolerance regulation, providing valuable insights for the development of hardy tomato varieties.
Natural variation in Tiller Number 1 affects its interaction with TIF1 to regulate tillering in rice
Authors: Zhang Quan , Xie Jianyin , Zhu Xiaoyang , Ma Xiaoqian , Yang Tao , Khan Najeeb Ullah , Zhang Shuyang , Liu Miaosong , Li Lin , Liang Yuntao , Pan Yinghua , Li Danting , Li Jinjie , Li Zichao , Zhang Hongliang , Zhang Zhanying 
Published: January 2023

SummaryTiller number per plant—a cardinal component of ideal plant architecture—affects grain yield potential. Thus, alleles positively affecting tillering must be mined to promote genetic improvement. Here, we report a Tiller Number 1 (TN1) protein harbouring a bromo‐adjacent homology domain and RNA recognition motifs, identified through genome‐wide association study of tiller numbers. Natural variation in TN1 affects its interaction with TIF1 (TN1 interaction factor 1) to affect DWARF14 expression and negatively regulate tiller number in rice. Further analysis of variations in TN1 among indica genotypes according to geographical distribution revealed that low‐tillering varieties with TN1‐hapL are concentrated in Southeast Asia and East Asia, whereas high‐tillering varieties with TN1‐hapH are concentrated in South Asia. Taken together, these results indicate that TN1 is a tillering regulatory factor whose alleles present apparent preferential utilization across geographical regions. Our findings advance the molecular understanding of tiller development.
Creating a quadruple aim model for nursing education
Authors: Krumwiede Kelly A. , Eardley Debra L. , DeBlieck Conni J. , Martin Karen S. 
Published: January 2023

AbstractBaccalaureate nursing education is moving to adopt the new American Association of Colleges of Nursing Essentials for Professional Nursing Education. As identified in two of the six domains of the essentials, graduates need to be prepared to address population health and utilize informatics and healthcare technologies. Community/public health nursing also has eight domains for generalist nurses linked to population health which will help prepare a skilled nursing workforce for the 21st century. The Institute for Healthcare Improvement's Triple Aim which evolved into the Quadruple Aim is focused on improving health outcomes within healthcare delivery. Through a literature review, a need for a Quadruple Aim model for nursing education was identified. Mirroring the Institute for Healthcare Improvement's Triple Aim for healthcare delivery, a Quadruple Aim for Nursing Education Model was developed. The model dimensions include (1) Population‐focused Care, (2) Maximize Student Learning Experience, (3) Cost‐effective Pedagogy, and (4) Nurse Educator Well‐being. The Quadruple Aim for Nursing Education Model supports nursing education to prepare future nurses effectively and efficiently bridging population health concepts and issues with nursing informatics. Nurse educators are encouraged to utilize the model to transform nursing education.
Combined effect of elevated temperature and drought stress on carbohydrate metabolism of cotton (Gossypium hirsutum L.) subtending leaves
Authors: Hu Wei , Gao Min , Du Kang , Liu Yang , Xu Bingjie , Wang Youhua , Zhou Zhiguo , Zhao Wenqing 
Published: January 2023

AbstractHigh temperatures and drought are expected to become more frequent in the future and last longer than ever before. To investigate their combined effect on leaves subtending cotton boll (LSCB), an experiment was conducted from 2016 to 2018 using a nonheat‐tolerant cotton cultivar and a heat‐tolerant cultivar. Two temperature regimes with ambient temperature (AT, 31.0/26.4°C) and elevated temperature (ET, 33.4/28.9°C, 2.5°C higher than AT) and three drought treatments with a soil relative water content (SRWC) of 75 ± 5%, 60 ± 5%, and 45 ± 5% were established repeatedly. ET decreased net photosynthetic rate (Pn), initial rubisco activity (4.1.1.39, RuBP) and cytosolicfructose‐1,6‐bisphosphatase (cy‐FBPase; 3.1.3.11) activity, upregulated GhSuSyA, and GhSuSyD expressions, and increased SuSy (2.4.1.13) activity, which led to the decline of the final starch and sucrose contents. Moreover, RuBP, Pn, and starch content all decreased with drop in SRWC levels, but the cy‐FBPase and SPS (2.4.1.14) activity increased, which in turn increased sucrose content. Under combined stresses, when the changing trends of ET and drought effects were the same, the decrease of Pn, RuBP, and starch content was greater than under single stress exposure. However, when the changing trends of ET and drought effects were adverse, the combined effects on indicators such as cy‐FBPase, SuSy, sucrose content were mostly similar to drought stress. These results indicate that the effect of drought on carbohydrate metabolism in LSCB is more prominent than ET. Thus, the drought effect for carbohydrate metabolism in LSCB may need more attention than ET under combined heat and drought stress.
Productivity premium of multinationals in global ownership linkages: A comparison of second‐tier subsidiaries
Authors: Tomiura Eiichi , Kumanomido Hiroshi 
Published: January 2023

AbstractHigh productivity of foreign direct investment (FDI) firms is a stylized fact. However, in the real world, there are subsidiaries owned by foreign parents but establishing their own foreign subsidiaries. Based on global ownership linkage data, we compare productivity levels of subsidiaries owned by parents in G‐7 countries but located worldwide. The FDI productivity premium is on average significantly small if investing firms are owned by foreign parents. Among firms with subsidiaries, the foreign ownership tends to reduce the FDI premium more when firms depend more on intangibles. This suggests that knowledge transfers within multinationals facilitate subsidiaries to make their own FDI.
Meeting Preferences for Specific Contraceptive Methods: An Overdue Indicator
Authors: Burke Kristen Lagasse , Potter Joseph E. 
Published: January 2023

AbstractFertility surveys have rarely asked people who are using contraception about the contraceptive method they would like to be using, implicitly assuming that those who are contracepting are using the method they want. In this commentary, we review evidence from a small but growing body of work that oftentimes indicates this assumption is untrue. Discordant contraceptive preferences and use are relatively common, and unsatisfied preferences are associated with higher rates of method discontinuation and subsequent pregnancy. We argue that there is opportunity to center autonomy and illuminate the need for and quality of services by building on this research and investing in the development of survey items that assess which method people would like to use, as well as their reasons for nonpreferred use. The widespread adoption of questions regarding method preferences could bring indicators of reproductive health services into closer alignment with the needs of the people they serve.
Predictive modeling of lower extremity injury risk in male elite youth soccer players using least absolute shrinkage and selection operator regression
Authors: Kolodziej Mathias , Groll Andreas , Nolte Kevin , Willwacher Steffen , Alt Tobias , Schmidt Marcus , Jaitner Thomas 
Published: January 2023

PurposeTo (1) identify neuromuscular and biomechanical injury risk factors in elite youth soccer players and (2) assess the predictive ability of a machine learning approach.Material and MethodsFifty‐six elite male youth soccer players (age: 17.2 ± 1.1 years; height: 179 ± 8 cm; mass: 70.4 ± 9.2 kg) performed a 3D motion analysis, postural control testing, and strength testing. Non‐contact lower extremities injuries were documented throughout 10 months. A least absolute shrinkage and selection operator (LASSO) regression model was used to identify the most important injury predictors. Predictive performance of the LASSO model was determined in a leave‐one‐out (LOO) prediction competition.ResultsTwenty‐three non‐contact injuries were registered. The LASSO model identified concentric knee extensor peak torque, hip transversal plane moment in the single‐leg drop landing task and center of pressure sway in the single‐leg stance test as the three most important predictors for injury in that order. The LASSO model was able to predict injury outcomes with a likelihood of 58% and an area under the ROC curve of 0.63 (sensitivity = 35%; specificity = 79%).ConclusionThe three most important variables for predicting the injury outcome suggest the importance of neuromuscular and biomechanical performance measures in elite youth soccer. These preliminary results may have practical implications for future directions in injury risk screening and planning, as well as for the development of customized training programs to counteract intrinsic injury risk factors. However, the poor predictive performance of the final model confirms the challenge of predicting sports injuries, and the model must therefore be evaluated in larger samples.
Raffinose positively regulates maize drought tolerance by reducing leaf transpiration
Authors: Liu Ying , Li Tao , Zhang Chunxia , Zhang Wenli , Deng Nan , Dirk Lynnette M. A. , Downie A. Bruce , Zhao Tianyong 
Published: January 2023

SUMMARYDrought stress is one of the major constraints of global crop production. Raffinose, a non‐reducing trisaccharide, has been considered to regulate positively the plant drought stress tolerance; however, evidence that augmenting raffinose production in leaves results in enhanced plant drought stress tolerance is lacking. The biochemical mechanism through which raffinose might act to mitigate plant drought stress remains unidentified. ZmRAFS encodes Zea mays RAFFINOSE SYNTHASE, a key enzyme that transfers galactose from the galactoside galactinol to sucrose for raffinose production. Overexpression of ZmRAFS in maize increased the RAFS protein and the raffinose content and decreased the water loss of leaves and enhanced plant drought stress tolerance. The biomass of the ZmRAFS overexpressing plants was similar to that of non‐transgenic control plants when grown under optimal conditions, but was significantly greater than that of non‐transgenic plants when grown under drought stress conditions. In contrast, the percentage of water loss of the detached leaves from two independent zmrafs mutant lines, incapable of synthesizing raffinose, was greater than that from null segregant controls and this phenomenon was partially rescued by supplementation of raffinose to detached zmrafs leaves. In addition, while there were differences in water loss among different maize lines, there was no difference in stomata density or aperture. Taken together, our work demonstrated that overexpression of the ZmRAFS gene in maize, in contrast to Arabidopsis, increased the raffinose content in leaves, assisted the leaf to retain water, and enhanced the plant drought stress tolerance without causing a detectable growth penalty.
Cysteinyl leukotriene receptor 1 is a potent regulator of the endosomal‐lysosomal system in the ARPE‐19 retinal pigment epithelial cell line
Authors: Koller Andreas , Brunner Susanne Maria , Preishuber‐Pflügl Julia , Runge Christian , Ladek Anja‐Maria , Reitsamer Herbert Anton , Trost Andrea 
Published: January 2023

AbstractThe endosomal‐lysosomal system is central for cell homeostasis and comprises the functions and dynamics of particular organelles including endosomes, lysosomes and autophagosomes. In previous studies, we found that the cysteinyl leukotriene receptor 1 (CysLTR1) regulates autophagy in the retinal pigment epithelial cell line ARPE‐19 under basal cellular conditions. However, the underlying mechanism by which CysLTR1 regulates autophagy is unknown. Thus, in the present study, the effects of CysLTR1 inhibition on the endosomal‐lysosomal system are analyzed in detail to identify the role of CysLTR1 in cell homeostasis and autophagy regulation. CysLTR1 inhibition in ARPE‐19 cells by Zafirlukast, a CysLTR1 antagonist, depleted the lysosomal pool. Furthermore, CysLTR1 antagonization reduced endocytic capacity and internalization of epidermal growth factor and decreased levels of the transferrin receptor, CD71. Serum starvation abolished the effect of Zafirlukast on the autophagic flux, which identifies the endocytic regulation of serum components by CysLTR1 as an important autophagy‐modulating mechanism. The role of CysLTR1 in inflammation and cell stress has been exceedingly studied, but its involvement in the endosomal‐lysosomal pathway is largely unknown. This current study provides new insights into basal activity of CysLTR1 on cellular endocytosis and the subsequent impact on downstream processes like autophagy.
Critical geoeconomics: A genealogy of writing politics, economy and space
Authors: Mallin Felix , Sidaway James D. 
Published: January 2023

AbstractTowards the end of the Cold War, the vocabulary of global power, space and economy received a qualitative update. Amongst the terms rapidly gaining prominence since the early 1990s has been the notion of geoeconomics, the coining of which has frequently been attributed to the strategist Edward N. Luttwak. In his interpretation, it signified a transition away from Cold War ideological and military geopolitical competition towards commerce and market‐based geo‐power. Over the past three decades, a ‘geoeconomics boom’ set in, characterised by think tanks and a varied body of politico‐economic literature making extensive use of the term. Conventionally treated as a neologism, the provenance and earlier iterations of geoeconomics, some dating back more than a century, have been largely ignored by both celebratory and critical accounts. In this paper, we trace and contextualise these earlier instances, leading us to the Geopolitik era in Germany and references to geoeconomics in the United States in the decades after WWII. We thereby offer a critical genealogy of geoeconomics, conceptualised as an object of definitional struggle. Proponents of the term sought to position it variously as a tool of national economic cohesion and competition or as a way of understanding and harnessing shifting global power relations, whilst others sought to subsume geoeconomics to geopolitics. These past struggles track forward into ongoing dialectical tensions between geoeconomics and geopolitics as competing but related geostrategic visions.
Performance Assessment of Chemical Kinetics Neural Ordinary Differential Equations in Pairwise Mixing Stirred Reactor
Authors: Bansude Shubhangi , Imani Farhad , Sheikhi Reza 
Published: January 2023

Abstract
The present study aims to assess the potential of the neural ordinary differential equations (NODE) network for reliable and computationally efficient implementation of chemistry in combustion simulations. Investigations are performed using a hydrogen-air pairwise mixing stirred reactor (PMSR). The PMSR is a zero-dimensional case affordable to study combustion chemistry entailing a similar numerical solution procedure as probability density function methods for turbulent combustion simulations. A systematic approach is presented to apply the NODE, solely trained on canonical constant pressure homogeneous reactor data, to predict complex chemistry and mixing interactions in PMSR. The reactor involves combustion of hydrogen in air described by a finite-rate mechanism with 9 chemical species and 21 reaction steps. The NODE network is shown to accurately capture the evolution of thermochemical variables for different mixing and chemical timescales. It also exhibits a significant reduction in numerical stiffness resulting in improving the computational efficiency and enabling the use of explicit solvers for the integration of chemical kinetics. The assessment results based on PMSR show that compared to direct integration of detailed kinetics, the NODE can achieve significant computational time speedup for a comparable accuracy.
A 3-D Printed Optically Clear Rigid Diseased Carotid Bifurcation Arterial Mock Vessel Model for Particle Image Velocimetry Analysis in Pulsatile Flow
Authors: Stanley Nicholas , Ciero Ashley , Timms William , Hewlin Rodward L. 
Published: January 2023

Abstract
In recent years, blood flow analyses of diseased arterial mock vessels using particle image velocimetry (PIV) have been hampered by the inability to fabricate optically clear anatomical vessel models that realistically replicate the complex morphology of arterial vessels and provide highly resolved flow images of flow tracer particles. The aim of this paper is to introduce a novel approach for producing optically clear 3-D printed rigid anatomical arterial vessel models that are suitable for PIV analysis using a common 3-D inkjet printing process (using a Formlabs Form 2 3-D printer) and stock clear resin (RS-F2-GPCL-04). By matching the index of refraction (IOR) of the working fluid to the stock clear resin material, and by printing the part in a 45-deg print orientation, a clear anatomical model that allows clear visualization of flow tracer particles can be produced which yields highly resolved flow images for PIV analyses. However, a 45-deg print orientation increases the need for post-processing due to an increased amount of printed support material. During post-processing, the part must be wet sanded in several steps and surface finished with Novus Plastic Polish 3 Step System to achieve the final surface finish needed to yield high-resolution flow images. The mock arterial vessel model produced in this work is a 3-D printed diseased carotid bifurcation artery developed from CTA scan data. A PIV analysis was conducted on the developed mock arterial vessel model installed in a complex transient flow loop to assess the flow profiles within the model and the clarity of the model. A computational fluid dynamics (CFD) simulation was conducted on the same carotid bifurcation arterial geometry, and the results were used as a benchmark comparison for PIV results. The results obtained in this work show excellent promise for using the developed approach for developing 3-D printed anatomical vessel models for experimental PIV analyses. The fabrication methodology of the clear anatomical models, PIV results, and CFD results is described in detail.
Marine biogenic emissions of benzene and toluene and their contribution to secondary organic aerosols over the polar oceans
Authors: Wohl Charel , Li Qinyi , Cuevas Carlos A. , Fernandez Rafael P. , Yang Mingxi , Saiz-Lopez Alfonso , Simó Rafel 
Published: January 2023

Reactive trace gas emissions from the polar oceans are poorly characterized, even though their effects on atmospheric chemistry and aerosol formation are crucial for assessing current and preindustrial aerosol forcing on climate. Here, we present seawater and atmospheric measurements of benzene and toluene, two gases typically associated with pollution, in the remote Southern Ocean and the Arctic marginal ice zone. Their distribution suggests a marine biogenic source. Calculated emission fluxes were 0.023 ± 0.030 (benzene) and 0.039 ± 0.036 (toluene) and 0.023 ± 0.028 (benzene) and 0.034 ± 0.041 (toluene) μmol m
−2
day
−1
for the Southern Ocean and the Arctic, respectively. Including these average emissions in a chemistry-climate model increased secondary organic aerosol mass concentrations only by 0.1% over the Arctic but by 7.7% over the Southern Ocean, with transient episodes of up to 77.3%. Climate models should consider the hitherto overlooked emissions of benzene and toluene from the polar oceans.
Membranous nephropathy after a recent SARS-CoV-2 infection
Authors: Mateus Catarina , Theias Manso Rita , Martins Ana Rita , Branco Patrícia Quadros 
Published: January 2023

SARS-CoV-2 infections can induce kidney injury and glomerulopathy, with the most common pathology findings being acute tubular injury and collapsing glomerulopathy.Here we describe a rare case of membranous nephropathy in a man in his late 70s presented with nephrotic syndrome and rapidly progressive kidney dysfunction 1 month after SARS-CoV-2 infection. Phospholipase A2 receptor antibodies were positive. He was treated with rituximab, with proteinuria control. We review the cases reported in the literature.
Relationship between ethnicity and stage at diagnosis in England: a national analysis of six cancer sites
Authors: Fry Anna , White Becky , Nagarwalla Diana , Shelton Jon , Jack Ruth H 
Published: January 2023

ObjectivesCancer stage at diagnosis is a determinant of treatment options and survival. Previous research has shown differences in barriers to presentation with cancer between ethnic groups. The completeness and quality of cancer stage and ethnicity data has improved markedly over recent years in England, allowing for comparison of stage distributions at diagnosis between ethnic groups. This study aimed to assess relationships between ethnic group and two outcomes: unknown stage cancer and late stage (stages 3 and 4) cancer, after adjustment for confounders.Design and settingA retrospective secondary data analysis using data from NHS Digital’s National Cancer Registration and Analysis Service and Hospital Episode Statistics records from 2012 to 2016.ParticipantsThis study analysed newly diagnosed breast, colon, non-small cell lung cancer (NSCLC), ovary, prostate and uterine cancers in white British, Caribbean, African, Chinese and Asian patients aged 15–99 in England.ResultsCaribbean, African and Asian women with breast or ovarian cancer, Caribbean and African women with uterine or colon cancer, Caribbean women with NSCLC and Caribbean men with colon cancer had increased odds of late-stage disease at diagnosis compared with the white British cohort. In contrast, Caribbean and African men with prostate cancer had decreased odds of late-stage cancer. Where stage was known, there were variations in late-stage cancer by ethnic group.ConclusionsLow symptom awareness and barriers to presentation can cause delays, resulting in later stage diagnosis. Targeted intervention campaigns to help raise awareness of cancer signs and symptoms and the benefits of early diagnosis, along with removing barriers to appropriate referrals, could help to improve these inequalities.