bluuebunny/crossref_metadata_2025_split huggingface dataset:
Local Moho distribution in the eastern Mediterranean region from gravity inversion: eastern Mediterranean Sea
Authors: Harash Fayez , Khalaf Amin , Al-Ansari Nadhir , Chen Chao , Alrawi Imad , Al-Khafaji Harith , Tageldin Mohammed 
Published: June 2023

This study investigates the variations of surface Moho inverted from Bouguer gravity data beneath the northwestern Arabian plate. The results were obtained by applying the 3DINVER program developed in a MATLAB environment using the Parker–Oldenburg method. The calculation results show that the maximum Moho depth observed in the western Arabian shield approaches more than 40 km, while the minimum values of 16–20 km are mainly concentrated beneath the Mediterranean Sea. A key innovation of this study is its implementation of exponential density contrast decrease in the sediment–basement interface in the Syrian part of the Arabian plate. The gravity anomaly of the sediment is computed using a density contrast which varies exponentially with depth, leading to a more accurate representation of the subsurface structure and offering valuable insights into the geodynamic processes and seismogenic potential of the area. To validate the inverted results from the 3DINVER program, the study compares these findings with seismic results that show good agreement with our results from the same region. This leads to a better understanding of the crustal structure and provides insights into its implications for the geodynamic and seismogenic nature of the northwestern Arabian plate—critical for assessing the potential for earthquakes and other geological hazards. The density contrast between the Earth’s crust and mantle can be related to variations in the composition, temperature, and pressure of the rocks. These factors influence the mechanical properties of the crust, ultimately affecting the tectonic processes and seismic activity in the region.
Clinical manifestations and approach to the management of patients with common variable immunodeficiency and liver disease
Authors: Daza-Cajigal Vanessa , Segura-Guerrero Marina , López-Cueto María , Robles-Marhuenda Ángel , Camara Carmen , Gerra-Galán Teresa , Gómez-de-la-Torre Ricardo , Avendaño-Monje Carmen L. , Sánchez-Ramón Silvia , Bosque-Lopez María J. , Quintero-Duarte Adriana , Bonet-Vidal María L. , Pons Jaime 
Published: June 2023

PurposeThe clinical spectrum of common variable immunodeficiency (CVID) includes predisposition to infections, autoimmune/inflammatory complications and malignancy. Liver disease is developed by a proportion of patients with CVID, but limited evidence is available about its prevalence, pathogenesis and prognostic outcome. This lack of evidence leads to the absence of guidelines in clinical practice. In this study, we aimed at defining the characteristics, course and management of this CVID complication in Spain.MethodsSpanish reference centers were invited to complete a cross-sectional survey. Thirty-eight patients with CVID-related liver disease from different hospitals were evaluated by a retrospective clinical course review.ResultsIn this cohort, abnormal liver function and thrombocytopenia were found in most of the patients (95% and 79% respectively), in keeping with the higher incidence of abnormal liver imaging and splenomegaly. The most common histological findings included nodular regenerative hyperplasia (NRH) and lymphocytic infiltration, which have been associated with portal hypertension (PHTN) leading to a poorer prognosis. Autoimmune/inflammatory complications occurred in 82% of the CVID patients that developed liver disease and 52% of the patients treated with immunomodulators showed a reduction in the liver function tests’ abnormalities during treatment. Among the experts that conducted the survey, there was 80% or more consensus that the workup of CVID-related liver disease requires liver profile, abdominal ultrasound and transient elastography. The majority agreed that liver biopsy should be essential for diagnosis. There was 94% consensus that endoscopic studies should be performed in the presence of PHTN. However, there was 89% consensus that there is insufficient evidence on the management of these patients.ConclusionLiver disease varies in severity and may contribute substantially to morbidity and mortality in patients with CVID. Hence the importance of close follow-up and screening of this CVID complication to prompt early targeted intervention. Further research is needed to evaluate the pathophysiology of liver disease in patients with CVID to identify personalized treatment options. This study emphasizes the urgent need to develop international guidelines for the diagnosis and management of this CVID complication.
A novel visual brain-computer interfaces paradigm based on evoked related potentials evoked by weak and small number of stimuli
Authors: Xiao Xiaolin , Gao Runyuan , Zhou Xiaoyu , Yi Weibo , Xu Fangzhou , Wang Kun , Xu Minpeng , Ming Dong 
Published: June 2023

IntroductionTraditional visual Brain-Computer Interfaces (v-BCIs) usually use large-size stimuli to attract more attention from users and then elicit more distinct and robust EEG responses, which would cause visual fatigue and limit the length of use of the system. On the contrary, small-size stimuli always need multiple and repeated stimulus to code more instructions and increase separability among each code. These common v-BCIs paradigms can cause problems such as redundant coding, long calibration time, and visual fatigue.MethodsTo address these problems, this study presented a novel v-BCI paradigm using weak and small number of stimuli, and realized a nine-instruction v-BCI system that controlled by only three tiny stimuli. Each of these stimuli were located between instructions, occupied area with eccentricities subtended 0.4°, and flashed in the row-column paradigm. The weak stimuli around each instruction would evoke specific evoked related potentials (ERPs), and a template-matching method based on discriminative spatial pattern (DSP) was employed to recognize these ERPs containing the intention of users. Nine subjects participated in the offline and online experiments using this novel paradigm. ResultsThe average accuracy of the offline experiment was 93.46% and the online average information transfer rate (ITR) was 120.95 bits/min. Notably, the highest online ITR achieved 177.5 bits/min.DiscussionThese results demonstrate the feasibility of using a weak and small number of stimuli to implement a friendly v-BCI. Furthermore, the proposed novel paradigm achieved higher ITR than traditional ones using ERPs as the controlled signal, which showed its superior performance and may have great potential of being widely used in various fields.
Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion
Authors: Hu Haijun , Long Houqing , Ren Zhenxiao , Liu Tianhua , Xu Jinghui , Xiao Fan 
Published: June 2023

PurposeThe pain caused by spinal cord injury (SCI) poses a major burden on patients, and pain management is becoming a focus of treatment. Few reports have described changes in the brain after SCI. Particularly, the exact mechanism through which brain regions affect post-injury pain remains unclear. In this study, we aimed to determine the potential therapeutic mechanisms of pain. A mouse model of spinal cord contusion was established, and molecular expression in the anterior cingulate cortex (ACC) and periaqueductal gray (PAG) in the brain and animal behavior was observed after local injection of human umbilical cord mesenchymal stem cells (HU-MSCs) at the site of SCI.MethodSixty-three female C57BL/6J mice were divided into four groups: a sham operation group (n = 15); a spinal injury group (SCI, n = 16); an SCI + HU-MSCs group (n = 16) and an SCI + PBS group (n = 16), in which the SCI site was injected with HU-MSCs/phosphate buffer. The BMS score was determined, and the von Frey test and Hargreaves test were used to assess behavior every week after surgery. Mice were sacrificed in the fourth week after operation, and samples were collected. The expression of CGRP, Substance P, C-Fos and KCC2 in the ACC and PAG were observed with immunohistochemistry. Chromic cyanine staining was used to observe transverse sections of the injured spinal cord.ResultIn the ACC and PAG after SCI, the expression of CGRP, SP and C-Fos increased, and the expression of KCC2 decreased, whereas after HU-MSC injection, the expression of CGRP, SP and C-Fos decreased, and the expression of KCC2 increased. The SCI + HU-MSC group showed better exercise ability from 2 to 4 weeks after surgery than the SCI/SCI + PBS groups (P &lt; 0.001). Local injection of HU-MSCs significantly improved the mechanical hyperalgesia caused by SCI in the fourth week after surgery (P &lt; 0.0001), and sensation was significantly recovered 2 weeks after surgery (P &lt; 0.0001); no improvement in thermal hypersensitivity was observed (P &gt; 0.05). The HU-MSC group retained more white matter than the SCI/SCI + PBS groups (P &lt; 0.0001).ConclusionLocal transplantation of HU-MSCs at the site of SCI partially relieves the neuropathic pain and promotes recovery of motor function. These findings suggest a feasible direction for the future treatment of SCI.
Assessment of serum, dietary zinc levels, and other risk factors during the third trimester among pregnant women with and without pregnancy-induced hypertension: a case-control study
Authors: El Bilbeisi Abdel Hamid , Abo Khosa Sahar M. , Taleb Mahmoud H. , El Afifi Amany M. 
Published: June 2023

BackgroundThis study assessed serum, dietary zinc levels, and other risk factors during the third trimester among pregnant women with and without pregnancy-induced hypertension (PIH).MethodsThis case-control study was conducted in 2022, in the three main Obstetrics and Gynecology departments in Gaza Strip, Palestine. One hundred sixty pregnant women, during the third trimester, aged ≥20 years, were selected using a convenient sampling method. Data were obtained using an interview-based questionnaire, food frequency questionnaire, anthropometric measures, and biochemical tests. Statistical analysis was performed using SPSS version 24.ResultsThe participants’ mean age was 30.7 ± 5.6 years. A total of 47 (58.8%) of cases and 6 (7.5%) of controls were insufficiently active; and the mean of blood pressure (mmHg) was 133.3 ± 11.9/85.11 ± 10.0 for cases and 112.8 ± 9.5/68.02 ± 7.2 for controls with significant differences between the two groups (P = &lt;0.005). The mean serum zinc level (μg/dl) was 67.15 ± 16.5 for cases and 68.45 ± 18.0 for controls without significant differences between the two groups (P = 0.636). For newborns, the mean birth weight (g) was 2,904.6 ± 486 for cases, and 3,128.3 ± 501 for controls, and the mean Apgar score was 8.03 ± 0.62 for cases and 8.30 ± 1.17 for controls, with significant differences between the two groups (P = &lt;0.005). Furthermore, 43 (53.8%) of cases have family history of hypertension; 5 (6.2%) were primiparous; 19 (23.8%) have previous caesarian section; 33 (41.2%) have history of preeclampsia; and 62 (77.5%) have edema, with significant differences between the two groups (P = &lt;0.005). Additionally, the total zinc dietary daily intake (mg/day) was 4.15 ± 2.10 for cases and 4.88 ± 3.02 for controls, with significant differences between the two groups (P = 0.041). After adjustment for confounding variables, participants in the case group have higher odds of having low total zinc dietary intake compared to those in the control group [OR = 1.185, 95% CI = (1.016–1.382), P = 0.030].ConclusionThe current study showed the main risk factors of PIH among pregnant women in the Gaza Strip, Palestine. Furthermore, low maternal dietary zinc intake was associated with a high level of PIH. Moreover, having PIH could increase the risk of low birth weight and low Apgar scores. Therefore, reducing the main risk factors of PIH could reduce the adverse effect on both mother and birth outcomes.
An improved nomogram including elastography for the prediction of non-sentinel lymph node metastasis in breast cancer patients with 1 or 2 sentinel lymph node metastases
Authors: Duan Hongtao , Zhang Jiawei , Zhang Guanxin , Zhu Xingmeng , Wang Wenjia 
Published: June 2023

BackgroundThe rate of breast-conserving surgery is very low in China, compared with that in developed countries; most breast cancer patients receive mastectomy. It is great important to explore the possibility of omitting axillary lymph node dissection (ALND) in early-stage breast cancer patients with 1 or 2 positive sentinel lymph nodes (SLNs) in China. The aim of this study was to develop a nomogram based on elastography for the prediction of the risk of non-SLN (NSLN) metastasis in early-stage breast cancer patients with 1 or 2 positive SLNs.MethodsA total of 601 breast cancer patients were initially recruited. According to the inclusion and exclusion criteria, 118 early-stage breast cancer patients with 1 or 2 positive SLNs were finally enrolled and were assigned to the training cohort (n=82) and the validation cohort (n=36), respectively. In the training cohort, the independent predictors were screened by logistic regression analysis and then were used to conducted the nomogram for the prediction of NSLN metastasis in early-stage breast cancer patients with 1 or 2 positive SLNs. The calibration curves, concordance index (C-index), the area under the receiver operating characteristic (ROC) curve (AUC), and Decision curve analysis (DCA) were used to verified the performance of the nomogram.ResultsThe multivariable analysis showed that the enrolled patients with positive HER2 expression (OR=6.179, P=0.013), Ki67≥14% (OR=8.976, P=0.015), larger lesion size (OR=1.038, P=0.045), and higher Emean (OR=2.237, P=0.006) were observed to be the independent factors of NSLN metastasis. Based on the above four independent predictors, a nomogram was conducted to predict the risk of the NSLN metastasis in early-stage breast cancer patients with 1 or 2 positive SLNs. The nomogram showed good discrimination in the prediction of NSLN metastasis, with bias-corrected C-index of 0.855 (95% CI, 0.754-0.956) and 0.853 (95% CI, 0.724-0.983) in the training and validation cohorts, respectively. Furthermore, the AUC was 0.877 (95%CI: 0.776- 0.978) and 0.861 (95%CI: 0.732-0.991), respectively, indicating a good performance of the nomogram. The calibration curve suggested a satisfactory agreement between the predictive and actual risk in both the training (χ2 = 11.484, P=0.176, HL test) and validation (χ2 = 6.247, p = 0.620, HL test) cohorts, and the obvious clinical nets were revealed by DCA.ConclusionsWe conducted a satisfactory nomogram model to evaluate the risk of NSLN metastasis in early-stage breast cancer patients with 1 or 2 SLN metastases. This model could be considered as an ancillary tool to help such patients to be selectively exempted from ALND.
Physical, psychological, and behavioral problems among children and adolescents in countries with different economic statuses during the COVID-19 pandemic: a systematic review and meta-analysis
Authors: Peng Bo , Reeves Kara K. L. , Lee Shara W. Y. , Chung Tina H. Y. , Hui Heidi W. L. , Leung Alfred H. L. , Pang Johnson C. Y. 
Published: June 2023

IntroductionThe COVID-19 pandemic has impacted children and adolescents’ physical activity (PA), sleeping patterns, and psychological and behavioral health. Yet, little is known about the differences between those in countries with various economic statuses.MethodsArticles published from database inception through 16 March 2022 were retrieved using CINAHL Complete, Cochrane Library, EMBASE, Medline, PubMed, and PsycINFO. High-quality studies that reported the number of participants with parameters associated with PA, sleeping patterns, and psychological and behavioral problems in young people aged under 18 years during the pandemic were included. We referenced the Canadian 24-Hour Movement Guidelines for PA and sleep duration to provide the event rate for young people who were not compliant with the guidelines. The event rate of young people who had decreased sleep quality and experienced psychological and behavioral problems were also investigated. A subgroup analysis was conducted to identify the differences in those in countries with diverse economic statuses. Funnel plot analysis and Egger's test were also conducted to identify any risk of publication bias.ResultA total of 66 studies with 1,371,168 participants aged between 0 and 18 years, involving 27 countries, were included. During the pandemic, we identified that 41% (95% CI: 39%, 43%; I2 = 96.62) and 43% (95% CI: 34%, 52%; I2 = 99.42) of young people did not meet the PA and sleep duration recommendation guidelines. In addition, 31% (95% CI: 28%, 35%; I2 = 99.66) of young people had decreased their sleep quality. Yet, no significant difference was found across countries with different economic statuses. However, the event rates of participants with psychological and behavioral problems were 32% (95% CI: 28%, 36%; I2 = 99.85) and 19% (95% CI: 14%, 25%; I2 = 99.72), respectively. In addition, the rate of psychological problems was more severe in those who live in lower middle-income countries (p &lt; 0.001), while the rate of behavioral problems was more severe in those who live in high-income countries (p = 0.001).DiscussionDuring the pandemic, the discouragement of PA, poor sleep quality, and high risk of psychological and behavioral problems are concerning. A large number of young people did not comply with the recommendation guidelines. Timely implementation of recovery plans is critical to address the adverse effects on young people.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=309209, identifier CRD42022309209.
Impacts of climate change and human activities on vegetation coverage variation in mountainous and hilly areas in Central South of Shandong Province based on tree-ring
Authors: Yin Tingting , Zhai Yinuo , Zhang Yan , Yang Wenjun , Dong Jinbin , Liu Xiao , Fan Peixian , You Chao , Yu Linqian , Gao Qun , Wang Hui , Zheng Peiming , Wang Renqing 
Published: June 2023

IntroductionIt is of great significance to understand the characteristics and influencing factors of vegetation coverage variation in the warm temperate zone. As a typical region of the warm temperate zone in eastern China, the mountainous and hilly region in central-south Shandong Province has fragile ecological environment and soil erosion problem. Studying on vegetation dynamics and its influencing factors in this region will help to better understand the relationship between climate change and vegetation cover change in the warm temperate zone of eastern China, and the influence of human activities on vegetation cover dynamics.MethodsBased on dendrochronology, a standard tree-ring width chronology was established in the mountainous and hilly region of central-south Shandong Province, and the vegetation coverage from 1905 to 2020 was reconstructed to reveal the dynamic change characteristics of vegetation cover in this region. Secondly, the influence of climate factors and human activities on the dynamic change of vegetation cover was discussed through correlation analysis and residual analysis.Results and discussionIn the reconstructed sequence, 23 years had high vegetation coverage and 15 years had low vegetation coverage. After low-pass filtering, the vegetation coverage of 1911–1913, 1945–1951, 1958–1962, 1994–1996, and 2007–2011 was relatively high, while the vegetation coverage of 1925–1927, 1936–1942, 2001–2003, and 2019–2020 was relatively low. Although precipitation determined the variation of vegetation coverage in this study area, the impacts of human activities on the change of vegetation coverage in the past decades cannot be ignored. With the development of social economy and the acceleration of urbanization, the vegetation coverage declined. Since the beginning of the 21st century, ecological projects such as Grain-for-Green have increased the vegetation coverage.
Cellular response of Parachlorella kessleri to a solid surface culture environment
Authors: Miyauchi Hiroki , Ishikawa Tomoharu , Hirakawa Yutaro , Sudou Ayumu , Okada Katsuhiko , Hijikata Atsushi , Sato Norihiro , Tsuzuki Mikio , Fujiwara Shoko 
Published: June 2023

Attached culture allows high biomass productivity and is a promising biomass cultivating system because neither a huge facility area nor a large volume of culture medium are needed. This study investigates photosynthetic and transcriptomic behaviors in Parachlorella kessleri cells on a solid surface after their transfer from liquid culture to elucidate the physiological and gene-expression regulatory mechanisms that underlie their vigorous proliferation. The chlorophyll content shows a decrease at 12 h after the transfer; however, it has fully recovered at 24 h, suggesting temporary decreases in the amounts of light harvesting complexes. On PAM analysis, it is demonstrated that the effective quantum yield of PSII decreases at 0 h right after the transfer, followed by its recovery in the next 24 h. A similar changing pattern is observed for the photochemical quenching, with the PSII maximum quantum yield remaining at an almost unaltered level. Non-photochemical quenching was increased at both 0 h and 12 h after the transfer. These observations suggest that electron transfer downstream of PSII but not PSII itself is only temporarily damaged in solid-surface cells just after the transfer, with light energy in excess being dissipated as heat for PSII protection. It thus seems that the photosynthetic machinery acclimates to high-light and/or dehydration stresses through its temporal size-down and functional regulation that start right after the transfer. Meanwhile, transcriptomic analysis by RNA-Seq demonstrates temporary upregulation at 12 h after the transfer as to the expression levels of many genes for photosynthesis, amino acid synthesis, general stress response, and ribosomal subunit proteins. These findings suggest that cells transferred to a solid surface become stressed immediately after transfer but can recover their high photosynthetic activity through adaptation of photosynthetic machinery and metabolic flow as well as induction of general stress response mechanisms within 24 h.