bluuebunny/crossref_metadata_2025_split huggingface dataset:
Біологічне зварювання в хірургічному лікуванні хворих на МЛС-ТБ, пре-ШЛС-ТБ та ШЛС-ТБ
Authors: Іващенко В.Є. , Калабуха І.А. , Хмель О.В. , Волошин Я.М. 
Published: February 2023

Обґрунтування. Незважаючи на успіхи нових розробок і схем курсів хіміотерапії у хворих на туберкульоз із множинною (МЛС-ТБ), преширокою (пре-ШЛС-ТБ) і широкою лікарською стійкістю (ШЛС-ТБ), певна частина пацієнтів потребує хірургічного етапу лікування. З огляду на те що зазвичай на оперативне лікування хворі надходять після повного курсу консервативної терапії, тривалий запальний процес у зоні операції призводить до значних фіброзних змін у ділянці кореня та в плевральній порожнині. Для виконання етапів пневмолізу та розділення структур кореня ми з 2010 р. застосовуємо апарат біологічного зварювання ЕК-300М1. Раніше із цією метою використовували монополярну діатермічну коагуляцію.
Мета. Порівняти результати використання технології біологічного зварювання при операціях у пацієнтів із МЛС-ТБ, пре-ШЛС-ТБ та ШЛС-ТБ.
Матеріали та методи. Ми проаналізували результати лікування 135 хворих із МЛС-ТБ, пре-ШЛС-ТБ та ШЛС-ТБ, яким було виконано операції резекції легень у відділенні торакальної хірургії Національного інституту фтизіатрії і пульмонології з 2009 по 2022 р. Монополярну діатермію під час операції застосували 61 хворому, технологія біологічного зварювання використовувалася в 74 пацієнтів. За критерії ефективності для порівняння ми обрали такі показники: крововтрата, кількість інтраопераційних ускладнень, тривалість операції, кількість і тривалість післяопераційної ексудації та термін видалення дренажів, термін закінчення стаціонарного етапу лікування, середній термін перебування у відділенні реанімації й інтенсивної терапії, ступінь вираженості больового синдрому в ранньому післяопераційному періоді. Дослідження виконувалося коштом державного бюджету.
Результати. Встановлено, що в групі пацієнтів, у яких під час операції застосовувався апарат біологічного зварювання ЕК-300М1, скорочувалася тривалість операції на 17 %, крововтрата – на 22 %, зменшення процесів ексудації давало змогу видаляти внутрішньоплевральні дренажі на 1 добу раніше, термін закінчення стаціонарного етапу лікування скоротився на 13 %. Але кореляції зі ступенем вираженості больового синдрому та термінів лікування у відділенні реанімації й інтенсивної терапії при впровадженні цієї методики ми не виявили.
Висновки. Використання апарату біологічного зварювання ЕК-300М1 у ході оперативного лікування пацієнтів із МЛС-ТБ, пре-ШЛС-ТБ та ШЛС-ТБ достовірно покращило ефективність терапії та допомогло запобігти ускладненням. Пропонуємо ширше використовувати цю технологію для лікування вказаного контингенту хворих.
Gambaran Kapang Aspergillus Sp pada Terasi dalam Kemasan Tanpa Merek di Pasar Tradisional Kota Samarinda
Authors: Fitria Fitria , Suhartini Suhartini , Prihandono Dwi Setiyo 
Published: February 2023

Abstrak
Terasi merupakan bahan makanan hasil fermentasi hewani. Dalam fermentasi terasi terdapat mikroorganisme yang berperan penting pada prosesnya yakni bakteri Lactobacillus sp dan bakteri mesofil. Terasi dapat disimpan selama berbulan-bulan, tetapi kemasan terasi yang tidak diperhatikan tempat penyimpanannya bisa mengakibatkan terasi terkontaminasi oleh kapang. Kontaminasi terasi oleh kapang disebabkan karena terasi mengandung nutrisi, protein, vitamin dan mineral. Selain itu, terasi yang disimpan pada suhu dan kelembapan yang rendah dapat menyebabkan suburnya pertumbuhan kapang. Kapang Aspergillus sp dapat menyebabkan penyakit Aspergillosis pada manusia. Tujuan penelitian ini adalah untuk mengetahui gambaran kapang Aspergillus sp yang terdapat pada terasi dalam kemasan tanpa merek di pasar tradisional Kota Samarinda. Penelitian ini bersifat deskriptif dan sampel yang digunakan adalah terasi dalam kemasan tanpa merek dengan jumlah 24 sampel menggunakan teknik total sampling. Teknik analisis data menggunakan univariat. Hasil penelitian terasi dalam kemasan tanpa merek ditemukan Aspergillus sp sebanyak 11 (46%) jenis Aspergillus niger, Aspergillus flavus, dan Aspergillus fumigatus.
Kata kunci: aspergillus sp, kapang, terasi

Abstract
The shrimp paste is a fermented animal food ingredient. In shrimp paste fermentation there are microorganisms that play an important role in the process, namely Lactobacillus sp and mesophyll bacteria. The shrimp paste can be stored for months, but the shrimp paste packaging that is not properly stored can cause the shrimp paste to be contaminated by mold. Contamination of shrimp paste by mold is caused because shrimp paste contains nutrients, protein, vitamins and minerals. In addition, shrimp paste stored in low temperatures and humidity can lead to fertile mold growth. Aspergillus sp. can cause Aspergillosis in humans. The purpose of this study was to determine the description of the Aspergillus sp fungus found in unbranded shrimp paste in the traditional market of Samarinda. This research is descriptive and the sample used is unbranded shrimp paste with a total of 24 samples using a total sampling technique. Data analysis technique using univariate. The research found 11 (46%) Aspergillus sp species, Aspergillus niger, Aspergillus flavus, and Aspergillus fumigatus in unbranded shrimp paste.
Keywords: aspergillus sp, mold, shrimp paste
THE DEVELOPMENT OF THE INDONESIAN DEFENSE INDUSTRY BY USING SYSTEMS THINKING APPROACH
Authors: Kasim Kasim , Deksino George Royke 
Published: February 2023

<div><p class="Els-history-head">The independence of the national defense industry is crucial in supporting the national defense system to ensure the survival and existence of the nation and state through its impact on defense capability, and national economic, and technological development. But until now there are still phenomena that show the weaknesses of the national defense industry, both in concept and practice. This study aims to explain and analyze the weaknesses of the national defense industry and propose recommendations for solutions to overcome these problems. This study uses a qualitative approach, a methodology that involves interpretation to understand and explain certain phenomena. The analytical tool used is systems thinking, which can see this phenomenon in a bigger, dynamic, and interrelated picture through a system model. The results of this study indicate that the problem we are facing includes a small defense industry’s budget, lacking resources and technology, the need for raw materials, and pseudo-practices by the domestic defense industry. The proposed solution system model demonstrates how to develop the national defense industry by implementing the mandate of (Law of the Republic of Indonesia Number 16 of 2012 Concerning the Defense Industry), introducing defense product innovation through national and international defense industry exhibitions to increase investment opportunities, and implementing (Law of the Republic of Indonesia Number 11 of 2020 Concerning Job Creation) to involve private industry in the development of a strategic defense industry. Finally, the independence of the national defense industry will realize operational readiness into real operational capabilities, so that the independence of national defense will be achieved and the integrity of the sovereignty of the Republic of Indonesia will be maintained. This condition will immediately build the spirit of nationalism of Indonesian citizens. </p></div>
THE USE OF THE KANSEI ENGINEERING METHOD IN THE DESIGN OF THE MULTIVARIANCE MOUNT WEAPON WIRELESS CONTROL SYSTEM
Authors: Suprayitno Prayit , Panjaitan Marsono , Ain Khusnul , Yasin Moh. 
Published: February 2023

<p>The Frogman Forces Command or Kopaska (<em>Komando Pasukan Katak</em>)<em> </em>is one of the special forces owned by the Indonesian Navy (TNI AL). Kopaska unit often carries out covert operations such as Amphibious Operations, Special Operations, and other supports to expedite Indonesian Navy operations. To support the implementation of these tasks, the Kopaska unit needs to be equipped with special equipment to carry out operational tasks. Currently, the Kopaska unit does not yet have a weapon mount that can be controlled remotely. This study proposes a multivariance weapon wireless system control that is designed to be used remotely by using a mobile application through the internet. A Multivariance Mount Weapon Wireless System Control was designed using the Kansei Engineering method to provide alternative tools to support Kopaska tasks more effectively and efficiently and be able to minimize material and personnel losses. The average value of the level of user satisfaction with the tool design has reached a fairly good position, namely 3.17 from the initial value of 1.36 (scale 1 to 4), so this new product is ready as an alternative to be produced. </p>
Pengaruh Kombinasi Tepung Wortel dan Tepung Jagung pada Pakan Buatan terhadap Pertumbuhan dan Gambaran Darah Ikan Mas Koi (Cyprinus carpio L)
Authors: Idha Suaida Ramli Ramli , Muskita Wellem H. , Idris Muhammad , Kurnia Agus 
Published: February 2023

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh kombinasi tepung wortel dan tepung jagung pada pakan buatan terhadap pertumbuhan dan gambaran darah Ikan Mas Koi (Cyprinus carpio L). Penelitian menggunakan metode rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Ikan Koi diberi pakan dengan tiga pakan formulasi dan satu pakan komersil sebagai pakan kontrol selama 60 hari pemeliharaan. Tiga pakan formulasi mengandung 10% tepung wortel (pakan A), 10% tepung jagung (pakan B), dan 5% tepung wortel + 5% tepung jagung (pakan C), dan pakan komersil (pakan D) diberikan pada Ikan Koi sebanyak dua kali sehari dengan dosis 5% bobot tubuh. Parameter yang diamati adalah pertumbuhan mutlak, rasio konversi pakan, efisiensi pakan, total leukosit, dan total eritrosit. Hasil penelitian menunjukkan bahwa pakan yang telah diberikan tidak memberikan pengaruh nyata terhadap pertumbuhan mutlak, rasio konversi pakan, efisiensi pakan, tingkat kelangsungan hidup, total leukosit dan total eritrosit. Pertumbuhan mutlak rata-rata Ikan Mas Koi berkisar antara7,40–11,63 g, rasio konversi pakan berkisar antara 4,94–7,82 g, efisiensi pakan berkisar antara 12,78-20,33 %, total leukosit berkisar antara 0,99-1,03×105sel.mm-3, total leukosit berkisar antara 6,00-9,30×105sel.mm-3. Kata kunci: Gambaran Darah, Ikan Koi, Pertumbuhan, Tepung Jagung, Tepung Wortel
Perbandingan Laju Pertumbuhan Anakan Ujung Thallus Kappaphycus alvarezii dan Eucheuma denticulatum Hasil Seleksi Klon yang Dipelihara pada Boxnet
Authors: Ariskanti Ariskanti , Rahman Abdul , Kasim Ma’ruf 
Published: February 2023

Bagian anakan ujung thallus pada rumput laut merupakan bagian yang tercepat pertumbuhannya dibanding dengan bagian tengah dan bagian pangkal thallus. Seleksi klon juga merupakan metoda bertahap dalam mendapatkan populasi klon rumput laut yang memiliki pertumbuhan tercepat dan relatif seragam. Penelitian ini bertujuan untuk membandingkan laju pertumbuhan hasil seleksi klon thallus K. alvarezii dan E. denticulatum yang dipelihara dalam wadah terproteksi boxnet. Penelitian dilaksanakan pada bulan November 2019 - Januari 2020, di Perairan Pantai Lakeba, Kota Bau-Bau Sulawesi Tenggara. Menggunakan bagian ujung dari masing-masing thallus hasil seleksi klon rumput laut K. alvarezii dan E. denticulatum sebagai bibit. Berat awal masing-masing bibit jenis rumput laut tersebut adalah 20 g per rumpun yang selanjutnya dipelihara dalam boxnet selama 35 hari dengan rancangan acak lengkap (RAL). Parameter yang diamati yaitu laju pertumbuhan spesifik, pertumbuhan mutlak, parameter kualitas air dan hubungan laju pertumbuhan spesifik rumput laut dengan parameter kualitas lokasi perairan budidaya. Hasil penelitian mununjukan bahwa rata-rata LPS E. denticulatum 2.65%.hari-1 sedangkan K. alvarezii 1.80%.hari-1. Pertumbuhan Mutlak E. denticulatum yakni sebesar 30.89 g, sedangkan K. alvarezii 14.00 g. Parameter kualitas air yang diperoleh yakni suhu 28-30°C, salinitas 34-35 ppt, kecerahan 100%, arus 0.11-0.16 m.s-1, pH 7, nitrat 0.025-0.035 mg.l-1 dan posfat 0.007-0.017 mg.l-1. Paramater kualitas air yang berpengaruh signifikan terhadap laju pertumbuhan spesifik rumput laut baik pada K. alvarezii maupun pada E. denticulatum adalah suhu (X1), salinitas (X2), nitrat (X5) dan fosfat (X6). Parameter kualitas lainnya yaitu kecepatan arus (X3) dan pH (X4) keduanya tidak berpengaruh nyata.Kata Kunci : Kappaphycus alvarezii, Eucheuma denticulatum, seleksi klon, pertumbuhan, boxnet.
Safety and feasibility of left atrial appendage inversion in swine: A proof-of-concept study for potential therapy to prevent embolic stroke
Authors: Wang Yanmin , Wang Mengjun , Guo Xiaomei , Han Ling , Kassab Ghassan 
Published: February 2023

Objective: Left atrial appendage (LAA) occlusion or exclusion has been used in patients with atrial fibrillation to prevent stroke, but the techniques and devices have shortcomings. This study aims to validate the safety and feasibility of a novel LAA inversion procedure.Methods: LAA inversion procedures were done in six pigs. Before the procedure and at 8 weeks postoperatively, heart rate, blood pressure, and electrocardiogram (ECG) were recorded. The serum concentration of atrial natriuretic peptide (ANP) was measured. The LAA was observed and measured by transesophageal echocardiogram (TEE) and intracardiac echocardiogram (ICE). At 8 weeks after LAA inversion, the animal was euthanized. The heart was collected for morphology and histology, including hematoxylin-eosin, Masson trichrome, and immunofluorescence staining.Results: TEE and ICE showed that LAA was inverted, and the inversion was maintained during the 8-week study duration. Food intake, body weight gain, heart rate, blood pressure, ECG, and serum ANP level were comparable before and after the procedure. Morphology and histological staining showed that there was no obvious inflammation or thrombus. Tissue remodeling and fibrosis were observed at the LAA inverted site.Conclusion: The inversion of LAA effectively eliminates the dead space of LAA and thus may reduce the risk of embolic stroke. The novel procedure is safe and feasible, but the efficacy in reducing embolization remains to be demonstrated in future studies.
Short-term fructose feeding alters tissue metabolic pathways by modulating microRNAs expression both in young and adult rats
Authors: Petito Giuseppe , Giacco Antonia , Cioffi Federica , Mazzoli Arianna , Magnacca Nunzia , Iossa Susanna , Goglia Fernando , Senese Rosalba , Lanni Antonia 
Published: February 2023

Dietary high fructose (HFrD) is known as a metabolic disruptor contributing to the development of obesity, diabetes, and dyslipidemia. Children are more sensitive to sugar than adults due to the distinct metabolic profile, therefore it is especially relevant to study the metabolic alterations induced by HFrD and the mechanisms underlying such changes in animal models of different ages. Emerging research suggests the fundamental role of epigenetic factors such as microRNAs (miRNAs) in metabolic tissue injury. In this perspective, the aim of the present study was to investigate the involvement of miR-122-5p, miR-34a-5p, and miR-125b-5p examining the effects induced by fructose overconsumption and to evaluate whether a differential miRNA regulation exists between young and adult animals. We used young rats (30 days) and adult rats (90 days) fed on HFrD for a short period (2 weeks) as animal models. The results indicate that both young and adult rats fed on HFrD exhibit an increase in systemic oxidative stress, the establishment of an inflammatory state, and metabolic perturbations involving the relevant miRNAs and their axes. In the skeletal muscle of adult rats, HFrD impair insulin sensitivity and triglyceride accumulation affecting the miR-122-5p/PTP1B/P-IRS-1(Tyr612) axis. In liver and skeletal muscle, HFrD acts on miR-34a-5p/SIRT-1: AMPK pathway resulting in a decrease of fat oxidation and an increase in fat synthesis. In addition, liver and skeletal muscle of young and adult rats exhibit an imbalance in antioxidant enzyme. Finally, HFrD modulates miR-125b-5p expression levels in liver and white adipose tissue determining modifications in de novo lipogenesis. Therefore, miRNA modulation displays a specific tissue trend indicative of a regulatory network that contributes in targeting genes of various pathways, subsequently yielding extensive effects on cell metabolism.
Structural and electronic properties of H2, CO, CH4, NO, and NH3 adsorbed onto Al12Si12 nanocages using density functional theory
Authors: Li Liu-Kun , Ma Yan-Qiu , Li Kang-Ning , Xie Wen-Li , Huang Bin 
Published: February 2023

In this study, the adsorption of gases (CH4, CO, H2, NH3, and NO) onto Al12Si12 nanocages was theoretically investigated using density functional theory. For each type of gas molecule, two different adsorption sites above the Al and Si atoms on the cluster surface were explored. We performed geometry optimization on both the pure nanocage and nanocages after gas adsorption and calculated their adsorption energies and electronic properties. The geometric structure of the complexes changed slightly following gas adsorption. We show that these adsorption processes were physical ones and observed that NO adsorbed onto Al12Si12 had the strongest adsorption stability. The Eg (energy band gap) value of the Al12Si12 nanocage was 1.38 eV, indicating that it possesses semiconductor properties. The Eg values of the complexes formed after gas adsorption were all lower than that of the pure nanocage, with the NH3–Si complex showing the greatest decrease in Eg. Additionally, the highest occupied molecular orbital and the lowest unoccupied molecular orbital were analyzed according to Mulliken charge transfer theory. Interaction with various gases was found to remarkably decrease the Eg of the pure nanocage. The electronic properties of the nanocage were strongly affected by interaction with various gases. The Eg value of the complexes decreased due to the electron transfer between the gas molecule and the nanocage. The density of states of the gas adsorption complexes were also analyzed, and the results showed that the Eg of the complexes decreased due to changes in the 3p orbital of the Si atom. This study theoretically devised novel multifunctional nanostructures through the adsorption of various gases onto pure nanocages, and the findings indicate the promise of these structures for use in electronic devices.
Severe influenza infection is associated with inflammatory programmed cell death in infected macrophages
Authors: Ferreira André C. , Sacramento Carolina Q. , Pereira-Dutra Filipe S. , Fintelman-Rodrigues Natália , Silva Priscila P. , Mattos Mayara , de Freitas Caroline S. , Marttorelli Andressa , de Melo Gabrielle R. , Campos Mariana M. , Azevedo-Quintanilha Isaclaudia G. , Carlos Aluana S. , Emídio João Vítor , Garcia Cristiana C. , Bozza Patrícia T. , Bozza Fernando A. , Souza Thiago M. L. 
Published: February 2023

IntroductionInfluenza A virus (IAV) is one of the leading causes of respiratory tract infections in humans, representing a major public health concern. The various types of cell death have a crucial role in IAV pathogenesis because this virus may trigger both apoptosis and necroptosis in airway epithelial cells in parallel. Macrophages play an important role in the clearance of virus particles, priming the adaptive immune response in influenza. However, the contribution of macrophage death to pathogenesis of IAV infection remains unclear.MethodsIn this work, we investigated IAV-induced macrophage death, along with potential therapeutic intervention. We conducted in vitro and in vivo experiments to evaluate the mechanism and the contribution of macrophages death to the inflammatory response induced by IAV infection.ResultsWe found that IAV or its surface glycoprotein hemagglutinin (HA) triggers inflammatory programmed cell death in human and murine macrophages in a Toll-like receptor-4 (TLR4)- and TNF-dependent manner. Anti-TNF treatment in vivo with the clinically approved drug etanercept prevented the engagement of the necroptotic loop and mouse mortality. Etanercept impaired the IAV-induced proinflammatory cytokine storm and lung injury.ConclusionIn summary, we demonstrated a positive feedback loop of events that led to necroptosis and exacerbated inflammation in IAV-infected macrophages. Our results highlight an additional mechanism involved in severe influenza that could be attenuated with clinically available therapies.
Co-expression of thermophilic pectinases in a single host for cost-effective pectin bioconversion into D-galacturonic acid
Authors: Flores-Fernández Carol N. , Cárdenas-Fernández Max , Lye Gary J. , Ward John M. 
Published: February 2023

Co-expression of enzymes allow to produce multiple enzymes in a single host, representing a cost-effective alternative in biocatalytic processes which can be used for pectin bioconversion. Pectin-rich biomass is an abundant by-product from the fruit and sugar industries that is usually disposed in landfill or sold as a low value feedstock. The aim of this work was to co-express a thermophilic pectin methyl esterase (PME) and exo-polygalacturonases (exo-PGs) in a single host for pectin bioconversion into D-galacturonic acid (GalA) using different pectic substrates such as apple, citrus and sugar beet pectin. To achieve this, a PME from Bacillus licheniformis (BLI09) with either an exo-PG from Thermotoga maritima (TMA01) or from Bacillus licheniformis (BLI04) were cloned in pETDuet-1 and co-expressed in E. coli BL21 (DE3). Four co-expression plasmids containing both pectinases were constructed and factors such as the effect of the genes’ cloning order and their expression were evaluated. Co-expression constructs 3 and 4 (pETDuet-TMA01-BLI09 and pETDuet-BLI04-BLI09, respectively) showed better expression of both pectinases compared to co-expression constructs 1 and 2 (pETDuet-BLI09-TMA01 and pETDuet-BLI09-BLI04, respectively). Co-expression constructs 3 and 4 were the most efficient for pectin bioconversion into GalA reaching 3 and 2.5 mM GalA, respectively from apple and citrus pectin after 4 h reaction. In conclusion, this work demonstrates that the co-expression of pectinases can potentially contribute to reduce the cost associated to their production and purification as well as to increase their applicability for exploiting pectin-rich biomass to obtain bio-based chemicals.