bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the Effect of Incorporating Slag Cement with Pre-wetted Lightweight Aggregate on Reducing Cracking of Concrete Bridge Decks
Authors: Hamid Waleed K. , Khoury Issam , Mandadapu Mahendra , Al Rikabi Fouad T. , Ali Hajir 
Published: February 2023

Abstract
Early-age cracking is a severe problem in concrete bridges because it reduces the durability of the superstructure. One of the techniques to minimize early-age cracks is the addition of pre-wetted lightweight aggregate (LWA). There is limited research regarding the effect of pre-wetted LWA on the early-age performance of concrete. This paper aims to evaluate the efficiency of incorporating slag cement with pre-wetted LWA to reduce the potential cracking of the concrete decks. Also, the effect of fly ash on the shrinkage properties of concrete was investigated. Mixtures used in this study consist of replacements of total aggregate with pre-wetted LWA and partial replacements of cement with slag cement or fly ash. The performance of concrete specimens containing slag cement with pre-wetted LWA was evaluated in terms of mechanical properties, plastic shrinkage, free shrinkage, and restrained shrinkage. The results showed that the tensile strength was not the most significant parameter affecting the cracking potential of the concrete. The cracking potential of the concrete was strongly related to the tensile strength and free shrinkage. It was observed that incorporating slag cement with pre-wetted LWA significantly enhances the early-age performance of concrete as the tested specimens showed no sign of cracks. The results also showed that the shrinkage of the mixtures containing coarse LWA was reduced by 43 % compared with the control mixture. Moreover, it was noted that the LWA with higher absorption increases plastic shrinkage cracks for concrete decks under severe conditions.
Modeling of the Three-Dimensional Point Cloud Based on the Binocular Stereovision and Structure Identification
Authors: Wang Peitao , Zhang Bo , Haishaer Daowuerjiang , Zhu Chencheng , Cai Meifeng 
Published: February 2023

Abstract
The existence of weak planes affects the stability of rock engineering, especially the failure modes of the surrounding rocks. Therefore, investigation of the structural planes of rock mass and the extraction of discontinuities is important for rock mechanics. In this work, the LenaCV binocular stereovision system was studied and applied to establish three-dimensional (3D) surface models. Calibration of the binocular camera parameters, optimal distance discussion, and 3D modeling optimization were conducted. The optimal measuring distance and the intrinsic parameters suitable for the LenaCV stereovision were discussed. Finally, the 3D reconstruction of two case studies was carried out and the 3D point cloud modeling was established. The structural discontinuity mapping in rock faces was also carried out. The feasibility of the binocular stereovision system, as well as the optimum baseline distance, was discussed and verified. The study results can provide a new platform for the economic investigation of 3D rock mass structures and provide a new approach for the efficient extraction of rock mass discontinuities.
Identification of Novel Mutations Associated with Bedaquiline Resistance in Mycobacterium Marinum
Authors: Wang Longlong , Xu Yufan , Wang Zhe 
Published: February 2023

Objective:
Because infections caused by nontuberculous mycobacteria (NTM) are rapidly increasing globally, a need exists for developing novel antibiotics and discovering the mechanism of resistance. This research was aimed at understanding the mechanism of bedaquiline resistance in the model NTM species Mycobacterium marinum (M. marinum).

Methods:
The Mycobacterium marinum M strain was subjected to mutant selection with different concentrations of BDQ. After three rounds of evolution, 58 BDQ-resistant mutants were isolated and subjected to WGS. The results were confirmed through PCR and Sanger sequencing.

Results:
We identified seven genetic mutations among these mutants. The highest drug resistance (6–10× MIC) was associated with a mutation in AtpB, the primary biochemical target of BDQ in Mtb. Numerous mutations and insertions mapped to the gene MMAR_1007(46/58), which encodes the homolog of Rv0678 (MmpR) in Mtb. More than 93% of mutants (54/58) contained a single mutation (G563A) in MMAR_4049, which encodes the integral membrane protein YrbE3A-1.

Conclusion:
Both target-based and efflux-based actions contribute to BDQ resistance in M. marinum. Our findings may aid in developing novel potent anti-NTM (BDQ-based) drug regimens and diagnostic assays for the detection of BDQ-resistant M. marinum.
Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion
Authors: Seibert Michael J. , Evans Chantell S. , Stanley Kevin S. , Wu Zhenyong , Chapman Edwin R. 
Published: February 2023

Synaptotagmin 9 (SYT9) is a tandem C2 domain Ca2+sensor for exocytosis in neuroendocrine cells; its function in neurons remains unclear. Here, we show that, in mixed-sex cultures, SYT9 does not trigger rapid synaptic vesicle exocytosis in mouse cortical, hippocampal, or striatal neurons, unless it is massively overexpressed. In striatal neurons, loss of SYT9 reduced the frequency of spontaneous neurotransmitter release events (minis). We delved into the underlying mechanism and discovered that SYT9 was localized to dense-core vesicles that contain substance P (SP). Loss of SYT9 impaired SP release, causing the observed decrease in mini frequency. This model is further supported by loss of function mutants. Namely, Ca2+binding to the C2A domain of SYT9 triggered membrane fusionin vitro, and mutations that disrupted this activity abolished the ability of SYT9 to regulate both SP release and mini frequency. We conclude that SYT9 indirectly regulates synaptic transmission in striatal neurons by controlling SP release.SIGNIFICANCE STATEMENTSynaptotagmin 9 (SYT9) has been described as a Ca2+sensor for dense-core vesicle (DCV) exocytosis in neuroendocrine cells, but its role in neurons remains unclear, despite widespread expression in the brain. This article examines the role of SYT9 in synaptic transmission across cultured cortical, hippocampal, and striatal neuronal preparations. We found that SYT9 regulates spontaneous neurotransmitter release in striatal neurons by serving as a Ca2+sensor for the release of the neuromodulator substance P from DCVs. This demonstrates a novel role for SYT9 in neurons and uncovers a new field of study into neuromodulation by SYT9, a protein that is widely expressed in the brain.
Cerebral Creatine Deficiency Affects the Timing of Oligodendrocyte Myelination
Authors: Rosko Lauren M. , Gentile Tyler , Smith Victoria N. , Manavi Zeeba , Melchor George S. , Hu Jingwen , Shults Nataliia V. , Albanese Chris , Lee Yichien , Rodriguez Olga , Huang Jeffrey K. 
Published: February 2023

Cerebral creatine deficiency syndrome (CCDS) is an inborn error of metabolism characterized by intellectual delays, seizures, and autistic-like behavior. However, the role of endogenously synthesized creatine on CNS development and function remains poorly understood. Here, magnetic resonance spectroscopy of adult mouse brains from both sexes revealed creatine synthesis is dependent on the expression of the enzyme, guanidinoacetate methyltransferase (GAMT). To identifyGamt-expressed cells, and howGamtaffects postnatal CNS development, we generated a mouse line by knocking-in a GFP, which is expressed on excision ofGamt. We found thatGamtis expressed in mature oligodendrocytes during active myelination in the developing postnatal CNS. Homozygous deletion ofGamtresulted in significantly reduced mature oligodendrocytes and delayed myelination in the corpus callosum. Moreover, the absence of endogenous creatine resulted in altered AMPK signaling in the brain, reduced brain creatine kinase expression in cortical neurons, and signs of axonal damage. Experimental demyelination in mice after tamoxifen-induced conditional deletion ofGamtin oligodendrocyte lineage cells resulted in delayed maturation of oligodendrocytes and myelin coverage in lesions. Moreover, creatine and cyclocreatine supplementation can enhance remyelination after demyelination. Our results suggest endogenously synthesized creatine controls the bioenergetic demand required for the timely maturation of oligodendrocytes during postnatal CNS development, and that delayed myelination and altered CNS energetics through the disruption of creatine synthesis might contribute to conditions, such as CCDS.SIGNIFICANCE STATEMENTCerebral creatine deficiency syndrome is a rare disease of inborn errors in metabolism, which is characterized by intellectual delays, seizures, and autism-like behavior. We found that oligodendrocytes are the main source of endogenously synthesized creatine in the adult CNS, and the loss of endogenous creatine synthesis led to delayed myelination. Our study suggests impaired cerebral creatine synthesis affects the timing of myelination and may impact brain bioenergetics.
LITERASI EKONOMI, KESEHATAN DAN PENDIDIKAN DI CISATA KABUPATEN PANDEGLANG BANTEN
Authors: H Musfiroh Nurlaili , Rubiyanah Rubiyanah , Jamali Nurul 
Published: February 2023

AbstractBased on the results of observations and field assessments, the main problem of the service location in Cisata District, Pandeglang Regency is tourism-based economic literacy, healthy life literacy for life expectancy is getting better and the growth of independent / underdeveloped villages. That's why it is important to carry out assistance efforts to encourage acceleration development and independence of local villagers. Namely, through literacy strengthening programs or illiteracy eradication, both reading, writing and arithmetic. This includes supporting a healthy environmental situation through the construction of public toilets / toilets and the provision of clean water (joint drilling of wells / villagers). As well as promoting tourism based on community local wisdom and natural beauty. The empowerment approach used is action research or Participatory Action Research (PAR). Of the three dimensions, namely economy, education and health, community empowerment actions show mixed results. There are optimal results for several programs such as community consultation and drilling for clean water. However, there are also some programs that are not yet optimal, such as lectures on economic empowerment of the ummah.Keywords: capital and literacy assistance; participatory action research; skills programsAbstrakBerdasarkan hasil pengamatan dan assessmen lapangan, yang menjadi masalah utama lokasi pengabdian di Kecamatan Cisata Kabupaten Pandeglang adalah melek perekonomian berbasis wisata, melek hidup sehat untuk usia harapan hidup semakin membaikdan pertumbuhan kemandirian desa terlambat/tertinggal.Oleh karena itulah penting melakukan upaya pendampingan untuk mendorong percepatan pembangunan dan kemandirian warga desa setempat. Yakni melalui program penguatan literasi atau pengentasan buta aksara, baik membaca, menulis dan berhitung. Termasuk mendukung situasi lingkungan hidup yang sehat melalui pembangunan WC/Toilet umum dan penyediaan air bersih (pengeboran sumur bersama/warga desa). Serta promosi wisata berbasis kearifan lokal masyarakat dan keindahan alam.Pendekatan pemberdayaan yang digunakan adalah riset aksiatau Participatory Action Research (PAR).Dari ketigalierasi, yakniekonomi, pendidikandankesehatan,aksi pemberdayaan masyarakat memperlihatkan hasil yang beragam. Terdapat hasil yang optimal untuk beberapa program seperti rembuk warga dan pengeboran air bersih. Namun terdapat pula beberapa program yang belum optimal, seperti ceramah pemberdayaan ekonomi ummat.Kata kunci: bantuan modal dan literasi; participatory action research; program keterampilan
PENGETAHUAN DAN SIKAP REMAJA ANGGOTA PUSAT INFORMASI DAN KONSELING REMAJA TERHADAP TIGA RISIKO ANCAMAN DASAR KESEHATAN REPRODUKSI
Authors: Naufi Bilqis , Amanah Siti , Fatchiy Anna 
Published: February 2023

AbstractIn order to overcome teenager problems regarding reproductive health, the government through the National Population and Family Agency (BKKBN) created a Generation Planing Program (GenRe) that aims to prevent teenagers from three basic problems in reproductive health (TRIAD KRR) which include sexuality, HIV/AIDS, and drugs. The GenRe program is hold by PIK R which is the Center for Information and Counseling and managed youth to provide information and counseling services about teenage reproductive health. This study aimed to analyze the responds of counseling to their knowledge and behaviours about TRIAD KRR in PIK R Ceria Sentul, Bogor. This research was conducted from October to November 2019. The whole 57 members of PIK R Ceria Sentul were the respondents of the research. The collected data includes the profile of PIK R Ceria Sentul and extension services that have been implemented in the area. Data were analyzed using Partial Least Square (PLS). the results showed that the level of knowledge of teenagers about reproductive health related to the ability take care of it and their comprehension about HIV/AIDS about understanding and the meaning, avoiding, and the caused risk is high with a score of 38.6 and 45.6. Analysis using Partial Least Square showed that extension services significantly impacts the level of knowledge of adolescents about TRIAD KRR.Keywords: attitude; counseling; health reproduction; knowledge; teenagersAbstrakDalam rangka mengatasi permasalahan remaja mengenai kesehatan reproduksi, pemerintah melalui Badan Kependudukan dan Keluarga Nasional (BKKBN) membuat program Generasi Berencana (GenRe) yang bertujuan untuk menghindarkan remaja dari tiga ancaman dasar kesehatan reproduksi remaja (TRIAD KRR) yang mencakup kesehatan reproduksi, HIV/AIDS, dan NAPZA. Wadah dari program GenRe adalah Pusat Informasi dan Konseling Remaja (PIK R) yang dikelola dari, oleh, dan untuk remaja guna memberikan pelayanan informasi dan konseling mengenai kesehatan reproduksi remaja. Penelitian ini bertujuan untuk menganalisis pengaruh penyuluhan terhadap tingkat pengetahuan dan sikap remaja mengenai TRIAD KRR di PIK R Ceria Sentul Kabupaten Bogor. penelitian ini dilaksanakan dari Oktober hingga November 2019. Seluruh anggota PIK R Ceria Sentul sebanyak 57 orang merupakan responden penlitan. Data yang dikumpulkan adalah profil PIK R Ceria Sentul dan layanan penyuluhan yang telah diterapkan di daerah tersebut. data dianalisis menggunakan Partial Least Square (PLS). Hasil penelitian menunjukkan bahwa tingkat pengetahuan remaja mengenai kesehatan reproduksi terkait cara menjaganya dan tingkat pengetahuan mengenai HIV/AIDS terkait pengertian, cara menghindari, dan bahayanya sangat tinggi dengan skor 38,6 dan 45,6. Analisis menggunakan Partial Least Square menunjukkan penyuluhan berpengaruh secara signifikan terhadap tingkat pengetahuan remaja mengenai TRIAD KRR. Kata kunci: kesehatan reproduksi; pengetahuan; penyuluhan; remaja; sikapDOI-
Re-examination of shear design provisions for high-strength longitudinally reinforced concrete beams
Authors: Yu Bo , Sang Bujiu , Tao Xiaolei , Li Bing 
Published: February 2023

The lack of information regarding the shear behaviour of high-strength longitudinally reinforced concrete beams without shear reinforcement (HRCBW) impedes design engineers from using the full yield strength of the material. In this work, current shear design provisions for HRCBW were re-examined based on the probability density function (PDF), confidence interval and confidence level. Based on the principal shear mechanism of beam and arch actions, a probabilistic shear capacity model for HRCBW was developed based on Bayesian theory and the Markov chain Monte Carlo method, taking into account both aleatory and epistemic uncertainties. Statistical characteristics (e.g. mean, standard deviation, distribution type) of the shear capacity of HRCBW were determined by the Kolmogorov–Smirnov test and statistical analysis. The accuracy and applicability of three major shear design provisions for HRCBW (ACI 318-19, BS EN 1992-1-1:2004 and fib Model Code 2010) were re-examined based on the PDF, confidence interval and confidence level.
Tendência Temporal de Incidência e Perfil Epidemiológico de Pessoas com HIV/Aids em Município Médio Porte do Estado do Paraná: 2002-2017
Authors: Müller Erildo Vicente , Martins Camila Marinelli , Kruger Alícia , De Mello Ana Mônica , Silva-Junior Manoelito Ferreira 
Published: February 2023

A dinâmica da epidemia de HIV/aids vem passando por modificações ao longo do tempo sendo um dos principais problemas de saúde pública no Brasil e no mundo. O objetivo foi verificar a tendência temporal e o perfil epidemiológico de pessoas vivendo com HIV/aids. O estudo de Coorte retrospectivo com dados de 1078 pessoas vivendo com HIV e AIDS (PVHA) em Terapia Antiretroviral (TARV) entre 2002 e 2017 no município de Ponta Grossa-PR, Brasil. Utilizou-se frequências absoluta, as categorias de exposição e as diferenças foram verificadas entre grupos com teste Kruskall-Wallys. A tendência temporal foi verificada com regressão de Poisson (p<0,05). O perfil das PVHA foi descrito pela análise de correspondência múltipla. Foram avaliados 1078 PVHA, sendo 55,9% eram homens brancos, com idade média de 35,9 anos, casados, heterossexuais, transmissão foi sexual e com baixa escolaridade. A taxa média de incidência foi de 20,8/100.000 habitantes, com incremento de 5% ao ano, sendo maior entre os homens (8%). Diante da dinamicidade da infecção, deve-se repensar formas de abordagem nas estratégias de detecção de casos e prevenção.
Avaliação do software QualiVida: Uso de indicadores de saúde para planejamento e controle de ações em comunidade tradicional
Authors: Neves Valéria Rodrigues , Prat Bernat Viñolas , Cambraia Rosana Passos 
Published: February 2023

A partir de indicadores de requerimento ambiental, social e de saúde, construídos com base no formulário utilizado por Agentes Comunitários de Saúde na Estratégia de Saúde da Família, o software de análise multicritério à decisão denominado QualiVida avalia as condições de vida de famílias e seu agrupamento em comunidades tradicionais, possibilitando a identificação dos índices de desenvolvimento local. O objetivo deste trabalho foi avaliar a funcionalidade do software QualiVida e a sua aplicabilidade no controle de ações de intervenção, evidenciando os seus potenciais usuários e contribuições. Trata-se de um estudo exploratório-descritivo, apoiado em um teste de usabilidade. A coleta de dados ocorreu via questionário, documentos e observação não-participante. Os dados quantitativos foram analisados e interpretados pela técnica de medida de tendência central, enquanto os dados qualitativos foram analisados pelo método descritivo. Dentre os resultados, destaca-se que os Agentes Comunitários de Saúde e Enfermeiros aprovaram o software com média de 4,5. O software permitiu observar que o índice de qualidade de vida da comunidade em estudo não está dentro de uma faixa mínima aceitável nos três requerimentos. Concluindo, afirmamos que o software sob avaliação possui utilidade e relevância pública, servindo de base para o alcance de objetivos e estratégias traçados pelo Ministério da Saúde do Brasil, além de potencializar ações de outras organizações na tomada de decisão e direcionamento de políticas públicas.
Thickness of Leaf Anatomical Tissue Dicot Ornamental Plants at Different Light Intensities
Authors: Daningsih Entin , Fransiska Nadia , Mardiyyaningsih Asriah Nurdini 
Published: February 2023

Different light intensities affect the leaf anatomy. This study aims to measure the thickness of the anatomical tissue of the leaves of six types of dicot ornamental plants at different light intensities. This study used a quantitative experimental method with a completely randomized factorial design (CRD-Factorial). This design consisted of two factors, namely the main factors were six types of plants, namely Codiaeum variegatum (L.) A. Juss., Bougainvillea glabra Choisy, Tabernaemontana divaricata (L.) R, Br. Ex Roem. & Schult., Syzygium paniculatum Gaertn, Excoecaria cochinchinensis Lour., and Aerva sanguinolenta BI. The second factor is the different light conditions (open and shading) with three replications. Parameters measured were total leaf anatomical tissue thickness, upper and lower epidermis, palisade, and spongy using a modified paraffin method from Johansen. Data were analyzed using the CRD-Factorial ANOVA model followed by the LSD test. Correlation done between palisade, spongy, and lower epidermis thickness with total tissue thickness. The results showed that the leaf anatomy of six types of dicot ornamental plants varied. All plants have one layer of upper and lower epidermis tissue. In Codiaeum variegatum (L.) A. Juss., Syzygium paniculatum Gaertn, and Excoecaria cochinchinensis Lour. has two layers of palisade tissue while the Bougainvillea glabra Choisy, Tabernaemontana divaricata (L.) R,Br. Ex Roem. & Schult. and Aerva sanguinolenta BI has one layer of palisade tissue. Meanwhile, the spongy tissue in all plants has one layer of tissue. The thickness of the leaf tissue decreased in the shading area along with the depletion of the palisade, spongy, and lower epidermis tissue as indicated by a correlation from moderately strong to very strong. Shrinkage of palisade tissue, sponges, and lower epidermis is a plant's attempt to get optimal light in shading areas. The more lower epidermis srink, the thickness of leaf become thin while the thickness of palisade and sponge varied so the thickness of total leaf from each species also varied.
The Influence of Service Quality and Company Image on Customer Satisfaction and Word of Mouth
Authors: Sugiharto Didik Priyo Sugiharto , Almanik Yustino Islami Almanik , Wiyarni Wiyarni 
Published: February 2023

The purpose of this research is to investigate the relationship among service quality, company image, customer satisfaction, and word of mouth. The population of this study is the parents of students in Avesiena Nature School Malang, East Java, Indonesia, which is 193 people. This study used saturated sample, it means the sample is the same number with the population. In order to examine the relationship both direct and indirect between service quality and company image on word of mouth, this study used path analysis. According to validity and reliability tests, the data that used in this study can be said valid and reliable. Results of this study show that service quality, company image, and customer satisfaction have a significant impact on word of mouth. Indirectly or through customer satisfaction, service quality and company image also have a significant effect.

Key words: Service Quality, Company Image, Customer Satisfaction, Word of Mouth