bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering
Authors: Parvin Nejad Shouka , Lecce Monica , Mirani Bahram , Machado Siqueira Nataly , Mirzaei Zahra , Santerre J. Paul , Davies John E. , Simmons Craig A. 
Published: April 2023

Abstract
Background
Constructs currently used to repair or replace congenitally diseased pediatric heart valves lack a viable cell population capable of functional adaptation in situ, necessitating repeated surgical intervention. Heart valve tissue engineering (HVTE) can address these limitations by producing functional living tissue in vitro that holds the potential for somatic growth and remodelling upon implantation. However, clinical translation of HVTE strategies requires an appropriate source of autologous cells that can be non-invasively harvested from mesenchymal stem cell (MSC)-rich tissues and cultured under serum- and xeno-free conditions. To this end, we evaluated human umbilical cord perivascular cells (hUCPVCs) as a promising cell source for in vitro production of engineered heart valve tissue.

Methods
The proliferative, clonogenic, multilineage differentiation, and extracellular matrix (ECM) synthesis capacities of hUCPVCs were evaluated in a commercial serum- and xeno-free culture medium (StemMACS™) on tissue culture polystyrene and benchmarked to adult bone marrow-derived MSCs (BMMSCs). Additionally, the ECM synthesis potential of hUCPVCs was evaluated when cultured on polycarbonate polyurethane anisotropic electrospun scaffolds, a representative biomaterial for in vitro HVTE.

Results
hUCPVCs had greater proliferative and clonogenic potential than BMMSCs in StemMACS™ (p < 0.05), without differentiation to osteogenic and adipogenic phenotypes associated with valve pathology. Furthermore, hUCPVCs cultured with StemMACS™ on tissue culture plastic for 14 days synthesized significantly more total collagen, elastin, and sulphated glycosaminoglycans (p < 0.05), the ECM constituents of the native valve, than BMMSCs. Finally, hUCPVCs retained their ECM synthesizing capacity after 14 and 21 days in culture on anisotropic electrospun scaffolds.

Conclusion
Overall, our findings establish an in vitro culture platform that uses hUCPVCs as a readily-available and non-invasively sourced autologous cell population and a commercial serum- and xeno-free culture medium to increase the translational potential of future pediatric HVTE strategies.

Graphical Abstract
This study evaluated the proliferative, differentiation and extracellular matrix (ECM) synthesis capacities of human umbilical cord perivascular cells (hUCPVCs) when cultured in serum- and xeno-free media (SFM) against conventionally used bone marrow-derived MSCs (BMMSCs) and serum-containing media (SCM). Our findings support the use of hUCPVCs and SFM for in vitro heart valve tissue engineering (HVTE) of autologous pediatric valve tissue. Figure created with BioRender.com.
Hermite–Hadamard and Fejér-type inequalities for strongly reciprocally (p, h)-convex functions of higher order
Authors: Li Han , Saleem Muhammad Shoaib , Ahmed Imran , Aslam Kiran Naseem 
Published: April 2023

AbstractIn this paper, we investigate the properties of a newly introduced class of functions, strongly reciprocally (p, h)-convex functions of higher order. We establish Hermite–Hadamard-type and Fejér-type inequalities for this class of functions. Additionally, we present fractional integral inequalities applicable to strongly reciprocally (p, h)-convex functions of higher order.
A reverse extended Hardy–Hilbert’s inequality with parameters
Authors: Luo Ricai , Yang Bicheng , Huang Xingshou 
Published: April 2023

AbstractIn this paper, by virtue of the symmetry principle, applying the techniques of real analysis and Euler–Maclaurin summation formula, we construct proper weight coefficients and use them to establish a reverse extended Hardy–Hilbert’s inequality with multi-parameters. Then, we obtain the equivalent forms and some equivalent statements of the best possible constant factor related to several parameters. Finally, we illustrate how the obtained results can generate some new reverse Hardy–Hilbert-type inequalities.
Non-intubated deep paralysis: a new anaesthesia strategy for vocal cord polypectomy
Authors: Fan Yichen , Chi Xiaoying , Zhu Danyan , Yin Jiemin , Liu Yaling , Su Diansan 
Published: April 2023

Abstract
Background
Vocal cord polyp is common otorhinolaryngological disease, traditionally treated by vocal cord polypectomy under a supporting laryngoscope with general anaesthesia. Although it is safe and controllable, it would cause some anaesthesia complications. Moreover, the complex process of general anaesthesia may significantly reduce surgical efficiency. Avoiding these problems remains an important issue.

Methods
All patients were subjected to the standard non-intubated deep paralysis (NIDP) protocol consisting of four phases. An emergency plan was launched when NIDP cannot be implemented successfully. Patient characteristics, blood gas and monitoring data were collected during NIDP. Data concerning satisfaction, complications and duration of anaesthesia and recovery were collected to assess its effectiveness.

Result
Among 20 enrolled patients, the success rate of NIDP was 95%. Only one patient failed in completing NIDP. Blood gas analysis revealed that the partial pressure of oxygen and carbon dioxide was maintained at safe levels. Monitoring during NIDP revealed fluctuations in mean arterial pressure between 110 and 70 mmHg, and the heart rate was stable at 60–100 beats per minute. The duration of anaesthesia and postoperative recovery were 13.0 ± 2.84 and 5.47 ± 1.97 min, respectively. All patients and surgeons were satisfied with NIDP, and no complications were detected before discharge.

Conclusion
NIDP can be safely applied to patients and can replace general anaesthesia in vocal cord polypectomy. It can significantly reduce the duration of anaesthesia and postoperative recovery. No anaesthesia complications occurred without intubation, and patients and surgeons were satisfied with NIDP.

Trial registrations
This single-centre, prospective study was registered on clinicaltrial.gov (NCT04247412) on 30th July 2020.
Assessment of inequality in the Common Agricultural Policy in Portugal
Authors: Viegas Miguel , Wolf Jan , Cordovil Francisco 
Published: April 2023

AbstractThe Common Agricultural Policy (CAP) continues to represent a substantial part of the European Union's budget. Although the second pillar is co-financed by national governments, European funds represent the vast majority of public spending on agriculture and rural development. In the case of Portugal, the CAP envelope for the current Multiannual Financial Framework (MFF 2021–2027) represents a package of €10 billion. Our article focuses on the distribution of CAP support. Based on a synthetic indicator of equity aligned with the European Union objectives of the CAP, we assess the current distribution of CAP support and estimate its distribution in the future according to the new rules that come into force in January 2023. According to the data analyzed, the major factor of inequality in the CAP in Portugal rests in the exclusion of a significant part of agricultural holdings. In this sense, it is necessary to understand the reasons for this exclusion in order to study measures so that CAP rules can contribute to a fairer distribution between farmers and between regions.
Examining ecosystem (dis-)services on liver fluke infection in rural Northeast Thailand
Authors: Wang Yi-Chen , Law Andrea , Namsanor Jutamas , Sithithaworn Paiboon 
Published: April 2023

Abstract
Background
The direct reliance of humans on and their interactions with freshwater ecosystems in the Lower Mekong Basin have given rise to parasitic infections, which is particularly prevalent in Northeast Thailand where raw fish consumption is practiced. This study examined the interactions between environments, ecosystem (dis-)services, human raw fish consumption habits, and raw fish dish sharing on liver fluke infection risk.

Method
Water fecal contents and the first intermediate snail host were sampled between June and September of 2019. One hundred twenty questionnaires were surveyed in two villages of different environmental surroundings, one next to a river and the other located inland, in Northeast Thailand. Multivariate regression analyses using linear mixed effect models assessed the influence of social, behavioral and perceptual factors on raw fish consumption frequency, willingness to avoid consumption and liver fluke infection status. Social network analysis compared the degree of raw fish dish sharing between the villages and assessed the probable influence of connections to fish procurement locations and sharing activities on liver fluke infection risk.

Results
High abundance of the first intermediate snail host and presence of fecal contamination in water could endanger both villages to ecosystem disservices of parasitic transmission. The river-side village relied more on provisioning ecosystem services than the inland village (29.7% vs. 16.1% of villages) to consume raw fish as their main source of protein. Males in both villages (64.5 and 40.4 days/year for the respective villages) are also likely to consume koi pla and pla som, higher risk fish dishes, more frequently than females (4.1 and 4.3 days/year for the respective villages). The consumption habits of both villages were driven mostly by deriving cultural ecosystem services. Participation in raw fish dish sharing activities significantly reduced the odds of an individual being willing to avoid the consumption (Odds ratio = 0.19). Network analysis suggested that river-side villagers had a more direct raw fish dish sharing interaction and they procured fish from multiple locations; these characteristics might potentially account for more liver fluke infected households in the village.

Conclusion
Villagers’ raw fish consumption is driven by deriving cultural ecosystem services, and the geographic settings of the villages potentially affect villagers’ fish procurement locations and infection risk. The findings underscore the linkages between villagers and their surrounding ecosystem environments as pertinent determinants for foodborne parasitic disease risk.

Graphical Abstract
AI literacy in K-12: a systematic literature review
Authors: Casal-Otero Lorena , Catala Alejandro , Fernández-Morante Carmen , Taboada Maria , Cebreiro Beatriz , Barro Senén 
Published: April 2023

AbstractThe successful irruption of AI-based technology in our daily lives has led to a growing educational, social, and political interest in training citizens in AI. Education systems now need to train students at the K-12 level to live in a society where they must interact with AI. Thus, AI literacy is a pedagogical and cognitive challenge at the K-12 level. This study aimed to understand how AI is being integrated into K-12 education worldwide. We conducted a search process following the systematic literature review method using Scopus. 179 documents were reviewed, and two broad groups of AI literacy approaches were identified, namely learning experience and theoretical perspective. The first group covered experiences in learning technical, conceptual and applied skills in a particular domain of interest. The second group revealed that significant efforts are being made to design models that frame AI literacy proposals. There were hardly any experiences that assessed whether students understood AI concepts after the learning experience. Little attention has been paid to the undesirable consequences of an indiscriminate and insufficiently thought-out application of AI. A competency framework is required to guide the didactic proposals designed by educational institutions and define a curriculum reflecting the sequence and academic continuity, which should be modular, personalized and adjusted to the conditions of the schools. Finally, AI literacy can be leveraged to enhance the learning of disciplinary core subjects by integrating AI into the teaching process of those subjects, provided the curriculum is co-designed with teachers.
A multi-energy inertia-based power support strategy with gas network constraints
Authors: Miao Cairan , Wang Qi , Tang Yi 
Published: April 2023

AbstractAn integrated energy system with multiple types of energy can support power shortages caused by the uncertainty of renewable energy. With full consideration of gas network constraints, this paper proposes a multi-energy inertia-based power support strategy. The definition and modelling of gas inertia are given first to demonstrate its ability to mitigate power fluctuations. Since partial utilization of gas inertia can influence overall gas network parameters, the gas network is modelled with an analysis of network dynamic changes. A multi-energy inertia-based power support model and strategy are then proposed for fully using gas-thermal inertia resources in integrated energy systems. The influence of gas network constraints on strategy, economy and power outputs is analyzed. Special circumstances where the gas network can be simplified are introduced. This improves the response speed and application value. The feasibility and effectiveness of the proposed strategy are assessed using a real scenario.
Safety and feasibility of a novel bi-directional portal vein access kit during transjugular intrahepatic portosystemic shunt creation
Authors: Kang Richard D. , Nezami Nariman , Park Peter , DePalma Anthony A. , Loya Mohammed F. , Mhaskar Rahul , Engel Chad , Zwiebel Bruce , Hoots Glenn , Shaikh Jamil 
Published: April 2023

Abstract
Background
Transjugular intrahepatic portosystemic shunt (TIPS) creation remains as one of the more technically challenging endovascular procedures. Portal vein access from the hepatic vein often requires multiple needle passes, which increases procedure times, risk of complications, and radiation exposure. With its bi-directional maneuverability, the Scorpion X access kit may be a promising tool for easier portal vein access. However, the clinical safety and feasibility of this access kit has yet to be determined.

Materials and methods
In this retrospective study, 17 patients (12 male, average age 56.6 ± 9.01) underwent TIPS procedure using Scorpion X portal vein access kits. The primary endpoint was time taken to access the portal vein from the hepatic vein. The most common indications for TIPS were refractory ascites (47.1%) and esophageal varices (17.6%). Radiation exposure, total number of needle passes, and intraoperative complications were recorded. Average MELD Score was 12.6 ± 3.39 (range: 8–20).

Results
Portal vein cannulation was successfully achieved in 100% of patients during intracardiac echocardiography-assisted TIPS creation. Total fluoroscopy time was 39.31 ± 17.97 min; average radiation dose was 1036.76 ± 644.15 mGy, while average contrast dose was 120.59 ± 56.87 mL. The average number of passes from the hepatic vein to the portal vein was 2 (range: 1–6). Average time to access the portal vein once the TIPS cannula was positioned in the hepatic vein was 30.65 ± 18.64 min. There were no intraoperative complications.

Conclusions
Clinical utilization of the Scorpion X bi-directional portal vein access kit is both safe and feasible. Utilizing this bi-directional access kit resulted in successful portal vein access with minimal intraoperative complications.

Level of evidence
Retrospective cohort.
Benefit From Fractionated Dose-Dense Chemotherapy in Patients With Poor Prognostic Ovarian Cancer: ICON-8 Trial
Authors: Colomban Olivier , Clamp Andrew , Cook Adrian , McNeish Iain A. , You Benoit 
Published: April 2023

PURPOSE An international meta-analysis identified a group of patients with advanced epithelial ovarian cancer (EOC) with a very poor survival because of two unfavorable features: (1) a poor chemosensitivity defined by an unfavorable modeled CA-125 ELIMination rate constant K (KELIM) score <1.0 with the online calculator CA-125—Biomarker Kinetics, and (2) an incomplete debulking surgery. We assumed that patients belonging to this poor prognostic group would benefit from a fractionated densified chemotherapy regimen. METHODS The data set of ICON-8 phase III trial (ClinicalTrials.gov identifier: NCT01654146 ), where patients with EOC were treated with the standard three-weekly, or the weekly dose-dense, carboplatin-paclitaxel regimens and debulking primary surgery (immediate primary surgery [IPS] or delayed primary [or interval] surgery [DPS]), was investigated. The association between treatment arm efficacy, standardized KELIM (scored as favorable ≥1.0, or unfavorable <1.0), and surgery completeness was assessed by univariate/multivariate analyses in IPS and DPS cohorts. RESULTS Of 1,566 enrolled patients, KELIM was calculated with the online model in 1,334 with ≥3 CA-125 available values (85%). As previously reported, both KELIM and surgery completeness were complementary prognostic covariates, and could be combined into three prognostic groups with large OS differences: (1) good if favorable KELIM and complete surgery; (2) intermediate if either unfavorable KELIM or incomplete surgery; and (3) poor if unfavorable KELIM and incomplete surgery. Weekly dose-dense chemotherapy was associated with PFS/OS improvement in the poor prognostic group in both the IPS cohort (PFS: hazard ratio [HR], 0.50; 95% CI, 0.31 to 0.79; OS: HR, 0.58; 95% CI, 0.35 to 0.95) and the DPS cohort (PFS: HR, 0.53; 95% CI, 0.37 to 0.76; OS: HR, 0.57; 95% CI, 0.39 to 0.82). CONCLUSION Fractionated dose-dense chemotherapy might be beneficial for patients belonging to the poor prognostic group characterized by lower tumor chemosensitivity assessed with the online calculator CA-125—Biomarker Kinetics and incomplete debulking surgery. Further investigation in the future SALVOVAR trial is warranted.
Transnasal Videoendoscopy for Preoperative Airway Risk Stratification: Development and Validation of a Multivariable Risk Prediction Model
Authors: Barclay-Steuart Alexander , Großhennig Hanna L. , Sasu Phillip , Wünsch Viktor A. , Stadlhofer Rupert , Berger Joanna , Stark Maria , Sehner Susanne , Zöllner Christian , Petzoldt Martin 
Published: April 2023

BACKGROUND:
Transnasal flexible videoendoscopy (TVE) of the larynx is a standard of care for the detection and staging of pharyngolaryngeal lesions in otorhinolaryngology. Patients frequently present with existing TVE examinations before anesthesia. Although these patients are considered high risk, the diagnostic value of TVE for airway risk stratification is currently unknown. How can captured images or videos be used for anesthesia planning, and which lesions are most concerning? This study aimed to develop and validate a multivariable risk prediction model for difficult airway management based on TVE findings and to determine whether the discrimination of the Mallampati score can be improved by adding this new TVE model.

METHODS:
This retrospective single-center development and validation study assessed 4021 patients who underwent 4524 otorhinolaryngologic surgeries at the University Medical Centre Hamburg-Eppendorf between January 1, 2011, and April 30, 2018, with electronically stored TVE videos and included 1099 patients who underwent 1231 surgeries. TVE videos and anesthesia charts were systematically reviewed in a blinded fashion. The Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis was used for variable selection, model development, and cross validation.

RESULTS:
The prevalence of difficult airway management was 24.7% (304/1231). Lesions at the vocal cords, epiglottis, or hypopharynx were not selected by the LASSO regression, while lesions at the vestibular folds (ß-coefficient 0.123), supraglottic region (ß-coefficient 0.161), arytenoids (ß-coefficient 0.063), and viewing restrictions on the rima glottidis that cover ≥50% of the glottis area (ß-coefficient 0.485) and pharyngeal secretion retention (ß-coefficient 0.372) were relevant risk factors for difficult airway management. The model was adjusted for sex, age, and body mass index. The area under the receiver operating characteristic curve (95% confidence interval) of the Mallampati score was 0.61 (0.57–0.65) and 0.74 (0.71–0.78) of the TVE model combined with Mallampati (P < .001).

CONCLUSIONS:
Stored images and videos from TVE examinations can be reused for the purpose of predicting risk associated with airway management. Vestibular fold, supraglottic, and arytenoid lesions are most concerning, especially if they are accompanied by secretion retention or restrict the glottic view. Our data indicate that the TVE model improves discrimination of the Mallampati score and might, therefore, be a useful addition to traditional bedside airway risk examinations.
Chasing the cheetah: how field biomechanics has evolved to keep up with the fastest land animal
Authors: Shield Stacey , Muramatsu Naoya , Da Silva Zico , Patel Amir 
Published: April 2023

ABSTRACT
Studying the motion of cheetahs – especially in the wild – is a technically challenging endeavour that pushes the limits of field biomechanics methodology. Consequently, it provides an interesting example of the scientific symbiosis that exists between experimental biology and the technological disciplines that support it. This article uses cheetah motion research as a basis to review the past, present and likely future of field biomechanics. Although the focus is on a specific animal, the methods and challenges discussed are broadly relevant to the study of terrestrial locomotion. We also highlight the external factors contributing to the evolution of this technology, including recent advancements in machine learning, and the influx of interest in cheetah biomechanics from the legged robotics community.
In vivo immunomodulatory effect and safety of MSC-derived secretome
Authors: Agung Nugrahaningsih Dwi Aris , Purwadi Purwadi , Sarifin Is , Bachtiar Indra , Sunarto Sunarto , Ubaidillah Ubaidillah , Larasati Ines , Satriyo Pamungkas Bagus , Setiasari Dicka Wahyu , Hasanah Maria Nur , At-thobari Jarir , Mubarika Sofia 
Published: April 2023

Background: Secretomes have been gaining interest in treating several diseases due to their pharmaceutical effects, such as the immunomodulatory effect. This study aimed to determine the immunomodulatory effect of secretomes derived from human umbilical cord mesenchymal stem cells (MSCs) and their safety. Methods: We conducted an in vivo immunomodulatory study using a carbon clearance assay. The safety of single-dose administration of secretome was done using fixed-dose methods of acute toxicity test. Results: The phagocytic index was higher in mice treated with secretome than in untreated mice. The acute toxicity study also showed that the administration of secretome derived from human umbilical cord MSCs did not change the mice’s body weight, physical examination results, organ weight, and gross anatomy examination. Conclusions: This study presents the potential of secretome derived from MSCs as a safe immunomodulatory agent.