bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of air pollution and meteorological conditions on dry eye disease among residents in a northeastern Chinese metropolis: A six-year crossover study in a cold region
Authors: Lu Cheng-Wei , Fu Jing , Liu Xiu-Fen , Cui Zhi-Hua , Chen Wei-Wei , Li Guo , Li Xiao-Lan , Ren Yu , Shao Fei , Hao Ji-Long 
Published: February 2023

Abstract
The purpose of this study is to first explore the associations among dry eye disease (DED), air pollution, and meteorological conditions in the cold region of a northeastern Chinese metropolis (i.e., Changchun). Data on ambient air pollutants and meteorological parameters as well as diagnosed DED outpatients during 2015–2021 were collected. The associations between DED and environmental factors were analysed at multiple time scales using various statistical methods (i.e., correlation, regression and machine learning). Among the 10809 DED patients (21617 eyes) studied, 64.60% were female and 35.40% were male. A higher frequency of DED was observed in March and April, followed by January, August and October. Individual and multiple factor models showed the positive importance of particles with aerodynamic diameters less than 10 µm (PM10), carbon monoxide (CO), and ozone (O3) among normal air pollutants and air pressure (AP), air temperature (AT) and wind speed (WS) among normal meteorological parameters. Air pollutants (PM10, nitrogen dioxide: NO2) and meteorological parameters (AT, AP) have combined impacts on DED occurrence. For the first time, we further explored the associations of detailed components of atmospheric particles and DED, suggesting potential emission sources, including spring dust from bare soil and roads and precursor pollutants of summer O3 formation from vehicles and industry in Northeast China. Our results revealed the quantitative associations among air pollutants, meteorological conditions and DED outpatients in cold regions, highlighting the importance of coordinated policies in air pollution control and climate change mitigation.
Five actions for five people: emergency cesarean section protocol
Authors: Mannella Paolo , Pancetti Federica , Giannini Andrea , Russo Eleonora , Montt-Guevara Magda , Simoncini Tommaso 
Published: February 2023

Abstract
Objective
To evaluate an operative protocol for emergency cesarean section to improve teamwork and reduce surgical setup time.
Methods
sixty-six health care operators working together in the delivery ward (gynecologists, midwives, anesthesiologists) simulated an emergency scenario applying a “five actions for each operator” protocol. For each simulation, the decision to delivery interval was considered and the perception of each operator as a team worker was analyzed with specific tests.
Results
the “five actions for five people” protocol significantly reduces the decision to delivery interval (p < 0.001) for emergency cesarean section. Simultaneously, a simple and codified scheme improves communication among team members, avoids overlapping roles, and improves the feeling of having helped the team by each operator
Conclusion
the use of a standardized, simple, and immediately usable protocol improves the performance of the delivery room team in terms of the urgency and quality of the operator's participation in the event. Procedures of this type should be favored within emergency obstetric settings.
Improving caregivers and physicians understanding about delirium in patients aged 75 and over in a non-geriatric inpatient unit: a study before-and-after a multimodal educational intervention
Authors: Salaun M. , Gavazzi G. , Forestier E. , Trinchero F. , Boussat B. , Drevet S. 
Published: February 2023

Abstract
Background: Delirium is a common geriatric syndrome which has a poor prognosis if not managed rapidly. Our main objective was to improve the knowledge of a non-geriatric care team concerning delirium in the older patients through a multimodal educational intervention in an Infectious Diseases Department.
Materials & Methods: We carried out a monocentric descriptive cohort study in an infectious diseases unit (France), with a retrospective phase (March to May 2021) and a prospective phase (October to December 2021). The multimodal educational intervention on delirium was conducted in September 2021. The primary endpoint was the difference in the percentage of correct responses from the Delirium Knowledge Questionnaire (DKQ) before and after the intervention. The secondary objectives concerned the progression of the knowledge of the Confusion Assessment Method (CAM), the different forms of delirium and the impact of the intervention on the frequency of diagnosis of delirium, on the patient care pathway and on the quality of care.
Results: The rate of DKQ correct answers of the 24 health caregivers included in the study improved significantly(89.3% vs 84.7%, p=0.02). The CAM was known by 1/5th of caregivers before the intervention and reached 83.3% after training. More than half of the caregivers were aware of all 3 forms of delirium after training. The frequency of delirium diagnosis did not change after the intervention. Prescription of physiotherapy became more frequent, while the use of the mobile geriatric unit or the scheduling of remote geriatric consultations did not change.
Conclusion: Our multi-modal educational intervention resulted in an improvement in caregivers' knowledge of the topic of delirium. Almost three quarters of caregivershad a knowledge about the CAM. Given the frequency of diagnosis and the severity and costs associated with the occurrence of delirium, it seems of major interest to integrate initial and ongoing training for carers on delirium.
Breaking the 25% Transparency and 15% Efficiency limits of Perovskite Solar Cells in a broad angular range of sun-light incidence
Authors: Lorusso Antonella , Masi Sofia , Triolo Claudia , Mariano Fabrizio , Muia Simone , Cannavale Alessandro , Duan Yu , Anni Marco , De Giorgi Maria Luisa , Patané Salvatore , Selmi Olfa , Mora-Seró Iván , De Leo Stefano , Mazzeo Marco 
Published: February 2023

Abstract
Realizing semi-transparent perovskite solar cells for building applications is a very hard goal not only because the absorption and the transmission of light through the device are two competitive processes, but also because the Power Conversion Efficiency (PCE) decreases with the angle of incidence of sunlight rays due to the increased reflection intensity. Moreover, an Average Visible Transmittance (AVT) of 25% and PCE of 15% have to be overcome simultaneously in a wide angular range in order to penetrate the market of smart windows. Here, through a rational management of the p and s light reflection modes of a dielectric/metal/dielectric used as top-electrode, we realize a semi-transparent FAPbI3 perovskite solar cell with an AVT value of 28% without the detriment of the PCE, that remains over 15% in a broad angular range up to 50° from normal light-incidence, thus breaking the benchmarks for a real building integration.
Evalution the Antifungal Activity of Plants and Compounds on Reducing Growth and Aflatoxin B1 Production of Aspergillus parasiticus and Aspergillus flavus
Authors: Moghadasi Fariba , Roudbarmohammadi Shahla , Amanloo Saied , Nikoomanesh Fatemeh 
Published: February 2023

Abstract
Background
Aspergillus species are the reason for the wide range of infections especially invasive fungal infections. The aim of this study was defined to evaluate the antifungal activity of plants and compounds including thyme, Aloevera, Thyme, carvacrol, and nano-encapsulation of carvacrol on growth and aflatoxin production of Aspergillus parasiticus and Aspergillus flavus.
Methods and Results
The minimum inhibitory concentrations (MICs) of extracts, carvacrol, nanocarvacrol, and fluconazole as control were determined according to Clinical and Laboratory Standards Institute (CLSI) by serial microdilution method. Then, the effect of inhibitory concentrations of the studied compounds on the amount of aflatoxin B1 production was evaluated by real time PCR and high-performance liquid chromatography (HPLC).Our results indicate that the Aspergillus parasiticus and Aspergillus flavusare sensitive to selected plants and compounds.
Conclusion
our result showed that we can be used the extracts of plants and their compounds with the help of nanotechnology to control the production of aflatoxin.
Effect of biomass particle size on yield and composition of bio-oil produced from Empty Palm Fruit Bunch
Authors: Nelson Ebitei Sintei , Sadare Olawumi , Okewale Akindele , Iyuke Sunny , Daramola Michael 
Published: February 2023

Abstract
Empty palm fruit bunch (EPFB), a byproduct of palm oil processing, can be used efficiently to produce renewable energy-rich products, thus stimulating a cleaner environment. In this study, the pyrolysis production of bio-oil and biochar was performed in a fixed bed reactor by depolymerizing the primary components of the EPFB biomass (cellulose, hemicellulose and lignin) using biomass particle size of 0.30 mm, 0.60 mm and 1.0 mm. Increasing the biomass particle size to 0.6 mm (600µm) from 0.3mm (300µm), resulted to a bio-oil increased yield of 4.7 wt.%. A further particle size increase to 1.0 mm (1000µm), reduced the bio-oil output by about 2.8 wt.%. Low heating rates and mass transfer limitations for biomass particles larger than 0.6mm relative to smaller particles impacted the production of the heavier components through the reduction in generation of volatile products, resulting in a decreased bio-oil yield. Biomass's particle sizes substantially affected the pyrolyzed products distribution. With proper characterization the stabilization and upgrading of the crude bio-oil can be improved and bio-oil utilized as a renewable fuel and for platform chemical extraction. Comprehensive chemical, structural and morphological analyzes of the EPFB biomass and pyrolyzed products were performed using Ultimate and Proximate analytical method and several other characterization techniques to elucidate the biomass and products composition and characteristics as fossil derived fuel substitute. The result of chemical analysis showed that the produced bio-oil has a high energy density but very viscous (3.4mm/s) and highly corrosive (pH of 2.6), probably due to the high concentration of carboxylic acids, phenols and phenolic. Bio-oil is inferior to diesel fuel due to its high flash point, low pH, high oxygen concentration, and high viscosity. However, it can be upgraded and be utilized as a sustainable renewable fuel and for extraction of platform chemical.
Peroxidase-catalyzed proximity labeling to survey the proteome of nanomaterial-cell interface during macropinocytosis-mediated internalization
Authors: Wei Yushuang , Gong Yao , Li Yue-Xuan , Guan Jibin , Chen Yue , Pang Hong-Bo 
Published: February 2023

Abstract
Nanomaterials often need to interact with proteins on the plasma membrane to get cross and access their intracellular targets. Therefore, to fully understand the cell entry mechanism, it is of vital importance to gain a comprehensive insight into the proteome at the interface when nanomaterials encounter the cells. However, only very few studies have focused on this aspect. Here, we reported a peroxidase-based proximity labeling method to survey the proteome at the nanoparticle (NP)-cell interface. Horseradish peroxidase (HRP) was conjugated to a variety of NPs and other ligand types while still being able to biotinylate the proteins surrounding NP (or ligand)-receptor complexes. Using two NP-based tracers for macropinocytosis (MP), which is highly relevant to NP internalization, we performed a proteomic survey and revealed the interface proteome difference between traditional and receptor-dependent MP. Moreover, our survey found that E-cadherin (CDH1), while not serving as the primary receptor, is present at the NP-cell interface and is functionally important for the cellular uptake of a wide variety of NPs. Overall, by integrating nanotechnology with proximity labeling, our study provides an approach to map the proteome of NP-cell interface for investigating the molecular mechanism of NP and macromolecule internalization into cells.
Cell aggregation prevents anoikis and induces CD44 cleavage by maintaining lipid raft integrity to promote triple negative breast cancer metastasis
Authors: li dong , park younhee , hemati hami , liu xia 
Published: February 2023

Abstract
Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype, and metastasis is the major cause of cancer morbidity and mortality. Therefore, it is urgent to discover novel therapeutic targets and develop effective treatments for this lethal disease. Circulating tumor cells (CTCs) are considered “seeds of metastasis”. Compared to single CTCs, our previous studies have demonstrated that CD44 homophilic interaction mediates CTC aggregation to enhance the stemness, survival and metastatic ability of aggregated cells. Importantly, the presence of CD44+ CTC clusters correlates with a poor prognosis in breast cancer patients. Here, we further investigated the underlying mechanism of how CD44-mediated cell aggregation promotes TNBC metastasis. We found that cell detachment, which mimics the condition when tumor cells detach from the extracellular matrix (ECM) to metastasize, induces lipid raft disruption in single cells, but lipid rafts integrity is maintained in aggregated cells. We further found that lipid rafts integrity in aggregated cells is required for Rac1 activation to prevent anoikis. In addition, CD44 and γ-secretase coexisted at lipid rafts in aggregated cells, which promotes CD44 cleavage and generates CD44 intracellular domain (CD44 ICD) to enhance stemness. Consequently, lipid rafts disruption inhibited Rac1 activation, CD44 ICD generation and metastasis. These data reveal a new mechanism of cell aggregation-mediated TNBC metastasis via maintaining lipid raft integrity after cell detachment. The finding provides a potential therapeutic strategy to prevent CTC cluster-initiated metastasis by disrupting lipid raft integrity and its-mediated downstream pathways.
Cytoprotective effects of hypoxia against oxidative stress in human lens epithelial cells by up-regulating Nrf2 expression
Authors: Wan Xiu-Hua , Hou Fang , Huang Qi-Ming 
Published: February 2023

Abstract
AIM: To investigate the protective mechanism that hypoxia may attenuate human lens epithelial cells (LECs) injury caused by oxidative stress.
METHODS: Human LECs were cultured in different atmospheric levels of O2 at different 4-Hydroxynonenal (4-HNE) concentrations and detected the viability by CCK-8. The apoptosis, reactive oxygen species (ROS), and senescent level were analyzed in both hypoxia and normoxia cultured LECs by Annexin V/PI staining, fluorescent probe (DCFH-DA), β-galactosidase staining, and BrdU incorporation assay respectively. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were performed to quantify the mRNA levels of genes-associated oxidative stress, antioxidant damage, and apoptosis. Protein blotting analyses were performed with antibodies specific to antioxidant and apoptosis-specific proteins. The expression level of endogenous Nrf2 was changed by transient transfection, and the cell viability was studied under different conditions after 4-HNE treatment.
RESULTS: The proliferative activity of LECs was inhibited by 4-HNE treatment in both normoxic and hypoxic conditions, but hypoxia can attenuate the injury of 4-HNE to LECs by comparing with normoxia (***P < 0.001). We found that hypoxia-cultured LECs can inhibit the apoptosis, ROS, and senescent level by comparing with normoxia-cultured LECs. The qRT-PCR assays showed that hypoxia promote LECs to up-express HIF-1α and Nrf2 (***P < 0.001) and its downstream target genes NQO1, GSTP and p53 mRNA (**P < 0.01, ***P < 0.001), and down-express caspase3 gene (**P < 0.01, ***P < 0.001). The hypoxia increased the expression of HIF-1α (*P < 0.05, **P < 0.01) and Nrf2 (**P < 0.01, ***P < 0.001) and its downstream target proteins NQO1, GSTP and p53 (*P < 0.05, **P < 0.01, ***P < 0.001), and reduced caspase3 (**P < 0.01, ***P < 0.001) protein expression by western blotting. Alteration of Nrf2 expression can eliminate the protective effect of hypoxia on 4-HNE induced injury.
CONCLUSION: The results suggested that the hypoxic condition was inhibited the injury effect of 4-HNE induced oxidative stress by up-expressing antioxidant gene Nrf2 and its downstream genes, including p53, and inhibit LECs apoptosis by reducing caspase3 expression in LECs, so activate the protective mechanism to LECs survival in oxidative stress.
Risk factors for newly-developed cardiovascular disease and quality of life during the COVID -19 pandemic: an analysis of the English Longitudinal Study of Ageing
Authors: Patel Mubarak , Uthman Olalekan 
Published: February 2023

Abstract
Introduction
The COVID-19 pandemic had a wide range of effects on the English population, including on health and quality of life due to the subsequent lockdown restrictions set.
Aims
To investigate longitudinal changes in developing cardiovascular disease (CVD) and how that affects quality of life from pre-pandemic and during two lockdowns in England, in older adults, and what factors are associated to this.
Methods
Wave 9 of the core English Longitudinal Study of Ageing (ELSA) and Waves 1 and 2 of the ELSA COVID-19 sub-study were used to investigate the factors associated to developing CVD between timepoints, and what factors alongside CVD are associated to quality of life.
Results
Higher age and depression were associated to newly-developed CVD from pre-COVID to both COVID sub-study waves. Additionally, BMI increased odds of CVD and physical activity decreased odds. Non-White ethnicity, depression, females, and developing CVD were lower associated to quality of life. Age and physical activity was associated with higher quality of life.
Discussion
Ethnicity was not associated with newly-developed CVD but was associated with quality of life. Other factors of important include age, depression, gender, and physical activity. Findings are informative for future risk stratification and treatment strategies, especially while the COVID-19 pandemic is ongoing.
Orientation-driven photosynthesized carbon belowground mediates intercropped peanut microbiota changes for pathogen resistance
Authors: Lu Jumeng , Chen Yan , Shen Yi , He Ganghui , Li Shiwen , Sun Bo 
Published: February 2023

Abstract
Background and Aims
Intercropping is a widely used agricultural practice to maintain agri-ecosystem function in intensive farmland. However, traditional combinations of tall and short plants in intercropping systems often sacrifice the health and productivity of short crops due to the planting orientations. Understanding how orientation-driven light intensity vertically allocate photosynthesized carbon and manipulate rhizosphere microbiota of short crops would benefit for the optimization of aboveground design to make use of the belowground microbiota for short plant health in diversified cropping systems.
Methods
In this study, we manipulated the legume and grass (peanut and maize, respectively, representing short and tall crops) row orientation (east‒west vs. north‒south) in an filed intercropping system and combined high-throughput sequencing and DNA stable isotope probing (DNA-SIP) technology to explore how intercropped orientation-driven photosynthesized carbon synthesis and allocation trigger peanut rhizosphere microbiota for pathogen antagonism.
Results
From field observations, we found that planting in the north-south orientation enhanced peanut photosynthesized carbon synthesis by up to 2.16 times in terms of photosynthetically active radiation compared to the east‒west orientation in situ. Using DNA-SIP technology, we demonstrated that high light intensity induced 192% more photosynthesized carbon to be released along the plant–root–rhizosphere axis. This released rhizosphere carbon selectively enriched the beneficial microorganism Burkholderia, which effectively suppressed the peanut pathogenic fungus Alternaria alstroemeriae in vitro to promote host plant growth.
Conclusion
Changing the orientation of intercropping can adjust the distribution of photosynthesized carbon in the rhizosphere by changing the light interception of crops. Peanuts will intercept more light in the north-south direction, resulting in more photosynthesized carbon being allocated to the peanut rhizosphere. These carbon contribute to the assembly of microbiota beneficial to peanut growth and pathogen resistance.
Community-based recommendations to reduce child stunting prevalence: A capital city region study in Central Lampung Regency, Indonesia
Authors: Zuraida Reni , Angraini Dian Isti , Sriwidiatm Otniel , Apriyanti Tania , - Firdawati 
Published: February 2023

Abstract
Background
Identification of growth-inhibiting factors in children continues to be a starting point for finding specific and appropriate recommendations in the world's anti-stunting efforts. An overview of the nutrition of affected children juxtaposed with the understanding of the nutrition of mothers/caregivers of toddlers will confirm the high number of cases in 2021 in Lampung Tengah Regency.
Objectives
This paper provides recommendations for handling stunting based on confirmed characteristics and information as an increase in stunting cases among children in Central Lampung Regency which occurs in 2021.
Methods
A cross-sectional study was conducted on a sample of 360 households with stunting children aged 0–59 months selected by purposive sampling and spread across three capital areas in Lampung Tengah Regency, Indonesia, namely households located in the capital district (A), near the capital (B), and far from the capital city (C). Anthropometry is measured using standard procedures and categorized manually based on national references. Data was collected using structured questionnaires and quantitatively analyzed through SPSS version 17.0. Qualitative studies were conducted through FGDs and in-depth interviews with health workers who are authorized to overcome stunting in their areas. Recommendations are a formulation of the results of quantitative and qualitative study.
Results
Majority stunting children in our study were male (50.83%) with an average age of over two years (31.4 months). More than half have %RDA categories for energy and carbohydrate deficits, and half have %RDA categories for fat deficits. A total of 111 mothers/caregivers of children had poor nutrition knowledge, and 237 others were in the moderate category, while the majority of them had moderate nutritional attitudes (45%). The recommendation to continue supplementary feeding (SF) recovery activities by increasing understanding of nutrition for mothers/caregivers of children is the main program that needs to be focused on by the local government in efforts to deal with stunting children in Central Lampung Regency.
Conclusions
Improving the current evidence-based nutrition package through the provision of SF recovery and the performance of mothers/caregivers and cadres in providing and supervising children's meals, as well as feedback to existing community-based nutrition programs will dramatically reduce the prevalence of stunting.