bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between microbial diversity and geochemistry of representative elements in the surface environment of the antimony mining area in Xikuangshan, China
Authors: Wang Can , Zhang Rui-yong , Xia Jin-lan , Liu Hong-chang , Liu Yue , Zhou Yu-hang , Nie Zhen-yuan , Shu Wen-sheng 
Published: January 2023

Abstract
Microbes are key mediators in maintaining environmental stability and the migration of geological elements. Although the effect of environmental factors on the geochemical distribution of elements and microbes in Xikuangshan have been studied, the connection between the above two remains to be clarified. In this study, the distribution of elemental forms, especially heavy metals, various geochemical conditions, and the microbial community compositions of the soil, sediment and water samples collected from different sites of Xikuangshan were investigated to study the correlation between representative elemental geochemistry and microbial diversity. The results show that the pH values of the soil samples were significantly lower than those of the sediment and water samples, and the soil had the highest microbial biomass. The contents of As and Sb in the samples are higher than those of other heavy metal elements, and the most abundant occurrence form of heavy metals is reducible. The most abundant type of the microorganisms is bacteria, followed by fungi and archaea. The correlation analyses between environmental factors and microbial diversities indicated that the microbial community structure composition of Xikuangshan was strictly correlated with the content and speciation of elements. The main factors impacting microbial diversity are pH and redox potential, as well as the content and occurrence forms of Sb, As and Fe. This study is of value for revealing the geochemical characteristics of heavy metal occurrence forms associated with microbial diversity in the Xikuangshan mine and for pollution control of Sb mines.
Evolution of pressure and cavitation in transition region walls for supercritical flow
Authors: Jamali Tohid , Manafpour Mohammad , Ebrahimnezhadian Hamzeh 
Published: January 2023

Abstract
Geometrical changes and high flow velocity cause flow separation and cavitation in the transition regions of hydraulic structures. A few studies have been conducted on the flow pressure and cavitation index in these regions, and the results need to be still improved. The present study examined the flow pressure and cavitation index variations for expansion angles between 0° and 10° and Froude numbers up to 20.1. Several relevant equations were also suggested to predict permissible angles in the transition regions. The results showed that negative pressure occurred at all lateral expansion angles except 0° when the Froude number was equal to or greater than ≥6.5. The cavitation phenomenon did not occur on the side walls for Froude number up to 4.49. However, the values of the cavitation index were reduced to less than the critical value for the Froude number of 14 when expansion angle was greater than 6°. The results also revealed that the side walls should not be expanded when Froude number was equal to or greater than 17.5. The occurrence of the cavitation on these walls substantially increased for Froude number of 20.1 even as expansion angle equals 0°.
Continuous discharge monitoring of the Mirim-São Gonçalo system by the index velocity rating curve method
Authors: Gonçalves George Marino Soares , Bartels Guilherme Kruger , Lima Luciana Shigihara , Boeira Lukas dos Santos , Collares Gilberto Loguercio 
Published: January 2023

Abstract
The São Gonçalo Channel, located in the south of Brazil, is responsible for connecting the Mirim Lagoon to the Patos Lagoon, constituting the largest coastal lagoon system in Latin America. The assessment of its hydraulic variables is necessary given the importance of this channel for the region. Thus, this study aimed to evaluate the performance of the index velocity rating curve (IVRC) method, from velocity measurements provided by horizontal static-type acoustic Doppler profilers (H-ADCPs). For the two sections analyzed in this study (GS1 and GS2), IVRC models were developed considering the integrated velocity cell (IVC) method; the multi-cell velocity (MCV) method; the joint use of IVC and MCV; and a stage-mean velocity rating curve. The results point to an r2 of 0.986 (IVC), 0.998 (IVC + MCV), 0.534 (stage-mean velocity) at GS1, and r2 of 0.986 (IVC), 0.995 (IVC + MCV), and 0.815 (stage-mean velocity) at GS2. In both GS1 and GS2, results showed significant gains – for different flow conditions – on continuous estimations of flow velocities and discharges when considering the MCV + IVC method. The IVRC model that presented the best fit allowed the development of a time-series of discharges in the studied sites with high reliability.
Hydrological drought in two largest river-connecting lakes in the middle reaches of the Yangtze River, China
Authors: Li Xianghu , Ye Xuchun , Li Zhen , Zhang Dan 
Published: January 2023

Abstract
Poyang and Dongting Lakes are two important river-connecting lakes with a complicated water system pattern in the middle reaches of the Yangtze River, China. Recently, extreme drought events occurred frequently in the two lakes. This study analyzed the characteristics and differences of hydrological droughts in Poyang and Dongting Lakes during the period of 1964–2016 and explored the correlation between drought and large-scale climate indices. The results showed that the hydrological droughts of Poyang and Dongting Lakes became increasingly serious. Especially after 2003, both lakes entered the dry season earlier and the intensity of drought was increased. The hydrological drought of Poyang Lake was more serious than that of Dongting Lake in spring (16.40%) and winter (14.26%), while the autumn drought in Dongting Lake (32.46%) was severer than that in Poyang Lake (27.65%). The spring droughts in the two lakes were significantly associated with droughts in their local catchments with the joint probabilities of 10.84 and 9.52%, while the autumn droughts were consistent with the hydrological droughts of the Yangtze River with large joint probabilities (26.39 and 27.76%). The changes in large-scale climate indices more significantly affected the drought in Poyang Lake than that in Dongting Lake, especially in autumn and winter.
Antibacterial properties of photochemically prepared AgTiO2 membranes
Authors: Che Abdul Rahim Azzah Nazihah , Hoshida Hisashi , Mestre Sergio , Kumakiri Izumi 
Published: January 2023

Abstract
Biofouling reduces the membrane performance and has become a problem in many applications. One of the strategies to reduce biofouling is to apply antibacterial materials to the membrane surface, which prevents the attachment and growth of microorganisms. In this study, the surface of flat ceramic supports was covered with TiO2 powder, and silver was applied by photoreduction using a CH3COOAg solution at room temperature. After the photoreduction, AgOx and metallic silver were found on the TiO2 as analyzed by XPS. While a negligible amount of silver was released from the prepared AgTiO2 membranes into water, the dissolution of silver was enhanced in a 0.09 M NaCl solution. The AgTiO2 membranes inhibited the growth of Escherichia coli in dark conditions. The inhibition cannot be explained only by the concentration of silver ions released from the membranes. Microscopic observation showed that direct contact with AgTiO2 kills E. coli. The results showed the possibility of improving the antibacterial activity of membranes by applying an AgTiO2 coating.
Succinimide and Saccharin-based Enzyme-activated Inhibitors of Serine Proteases
Authors: Martyn Derek C. , Moore Michael J.B. , Abell Andrew D. 
Published: January 2023

Abstract:
The inhibition of human leukocyte elastase (HLE). and other serine proteases, by succinimide and saccharin-based compounds is reviewed. The succinimide compounds are unique in that the inactivating species is generated within the enzyme active site via a molecular rearrangement. The related saccharin derivatives also inactivate serine proteases by an enzyme-activated mechanism. Those factors effecting the potency, selectivity and stability of these important classes of inhibitor are discussed.
Current Trends in the Development of Nitric Oxide Donors
Authors: Hou Y. -C. , Janczuk A. , Wang P. G. 
Published: January 2023

Abstract:
Nitric oxide (NO) is an important messenger molecule involved in many pathological and physiological processes within the mammalian body. Exogenous NO sources constitute a powerful way to supplement NO when the body can not generate enough for normal biological functions. In this article, general aspects on NO and NO donors are reviewed. Major focus is placed on recent developments of novel NO donors, NO releasing device(s) as well as innovative improvements to current NO donors. Finally, an outlook on future NO donor development is provided.
Transferrin As A Targeting Ligand for Liposomes and Anticancer Drugs
Authors: Singh M. 
Published: January 2023

Abstract:
In cancer treatment, one of the approaches is targeting of the drug to tumor cells via receptor specific ligands. Transferrin (molecular weight 80,000) has been used as a ligand for delivering anticancer drugs or drug containing liposomes mostly due to the increased number of transferrin (trf) receptors found on tumor cells as compared to normal cells. Transferrin was linked to methotrexate (MTX) containing small unilamellar liposomes and its activity was compared to antitransferrin receptor antibody (7D-3) linked to MTX liposomes. In each of these conjugates, the method of coupling was the same and a disulphide linkage was fanned between the ligand and MTX liposomes. No significant differences in the potency of 7D-3 conjugate or trf conjugate with MTX liposomes were observed in studies performed in vitro against various human tumor cell lines (Hela, KB and Colon). Trf was also linked to adriamycin via a schiff base which was formed by using glutaraldehyde. This conjugate was found to be effective in vitro against various human tumors (Lovo, HL-60, SW 403 and Hep2) and also in vivo against H­ mesothelioma tumors. Transferrin receptor has also been used for gene delivery. Gene delivery to K562 haematopoietic leukaemic cells was achieved by using a transferrin-polycation (poly-L-lysine or protamine) conjugate. This review will cover the various important applications of transferrin based drug delivery formulations in the chemotherapy of cancer and the related work performed in our and other laboratories.
Identification of Potential MicroRNA-MRNA Regulatory Relationship
Pairs in Irritable Bowel Syndrome with Diarrhea
Authors: Yan Wenli , Kan Zunqi , Li Zhaofeng , Ma Yuxia , Du Dongqing 
Published: January 2023

Background:
Irritable bowel syndrome (IBS) is the most common gastrointestinal disease
worldwide, with diarrhea-predominant irritable bowel syndrome (IBS-D) being the prevalent
subtype. However, its pathogenesis remains unclear. Research has increasingly focused on identifying
genetic factors in the mechanisms underlying IBS.

Objective:
We aimed to explore key gene nodes and potential microRNA-mRNA regulatory pairs of
IBS-D using bioinformatics methods.

Methods:
We downloaded the GSE36701 microarray dataset from the Gene Expression Omnibus
database and obtained 1358 differentially expressed mRNAs by analyzing mRNA profiles using the
GEO2R analysis tool. Based on our previous study, we used TargetScan, miTarBase, and miRDB to
predict the downstream genes of three known microRNAs (hsa-let-7b-5p, hsa-miR-19b-3p, and hsamiR-
20a-5p), and the microRNA-mRNA regulatory network was visualized using Cytoscape.

Results:
A total of 795 downstream target genes were found in TargetScan, miRTarBase, and
miRDB databases, and 50 candidate genes were obtained. The Metascape and STRING databases
were used to perform enrichment analysis and construct a protein-protein interaction network of
candidate genes. Finally, we constructed a network of 3 microRNAs and 50 candidate mRNAs,
among which 28 negative relation ship pairs and 5 key axes (hsa-miR-20a-5p/VEGFA, hsa-let-7b-
5p/MSN, hsa-let-7b-5p /PPP1R16B, hsa-19b-3p/ITGA2, and hsa-19b-3p/PIK3R3) were identified.

Conclusion:
We report five novel microRNA-mRNA regulatory axes in IBS-D pathogenesis and
speculated that PIK3R3, negatively regulated by hsa-miR-19b-3p, may regulate NF-κB production
through the PI3K/Akt pathway, which accounts for the occurrence of clinical symptoms in IBS-D
patients. Our findings may offer key biomarkers for IBS-D diagnosis and treatment.
Impact of Microgravity on Virulence, Antibiotic Resistance and Gene
Expression in Beneficial and Pathogenic Microorganisms
Authors: Salavatifar Maryam , Ahmadi Seyedeh Meysameh , Todorov Svetoslav Dimitrov , Khosravi-Darani Kianoush , Tripathy Abhishek 
Published: January 2023

Abstract:
During space missions, the impact of the space conditions (both microgravity and radiation)
on physiologic and metabolic aspects of the microbiota of astronauts' bodies should be considered.
Changes depend on the mission's duration, types of organisms, and ecology. Reported alterations
are related to changes in morphology, growth, gene expression, and physiology of cells, resulting in
increased virulence, acid, antibiotic resistance, biofilm formation, secondary metabolism, and microbial
mutations. Accordingly, recent research indicates the impacts of simulated microgravity on human
physiology and bacterial characteristics. This paper has reviewed the aspects of microgravity on
changes in microbiota, including virulence, antibiotic resistance, and gene expression. Microgravity
can undermine humans and makes influence bacterial pathogenicity. The review of papers shows that
some microorganisms showed higher pathogenicity under microgravity conditions. Moreover, sulfamethoxazole
had the highest resistance among Gram-positive microorganisms, and gentamicin had
the highest resistance in Gram-negative bacteria. All antibiotics reviewed under microgravity conditions
were robust in both groups of microorganisms compared to the gravity condition. Furthermore,
some gene expression was altered in bacteria under microgravity conditions compared to Earth conditions
(standard bacterial growth conditions). Changes in microbial behavior under microgravity directly
influence astronauts' health conditions, and a detailed analysis of known facts can provide essential
information for the selection of appropriate probiotics for these specific cases during the missions and
after the recovery processes. Moreover, the study of microorganisms changes in the absence of gravity
will help to understand the mechanisms of causing diseases on Earth and may be applied in clinical
practice.
Insight on Cardiac Chronobiology and Latest Developments of Chronotherapeutic
Antihypertensive Interventions for Better Clinical Outcomes
Authors: Joshi Kumud , Das Madhubanti , Sarma Anupam , Arora Mandeep K. , SInghal Manmohan , Kumar Bhavna 
Published: January 2023

Abstract:
Cardiac circadian rhythms are an important regulator of body functions, including car-diac activities and blood pressure. Disturbance of circadian rhythm is known to trigger and aggra-vate various cardiovascular diseases. Thus, modulating the circadian rhythm can be used as a ther-apeutic approach to cardiovascular diseases. Through this work, we intend to discuss the current understanding of cardiac circadian rhythms, in terms of quantifiable parameters like BP and HR. We also elaborate on the molecular regulators and the molecular cascades along with their specific genetic aspects involved in modulating circadian rhythms, with specific reference to cardiovascu-lar health and cardiovascular diseases. Along with this, we also presented the latest phar-macogenomic and metabolomics markers involved in chronobiological control of the cardiovascu-lar system along with their possible utility in cardiovascular disease diagnosis and therapeutics. Finally, we reviewed the current expert opinions on chronotherapeutic approaches for utilizing the conventional as well as the new pharmacological molecules for antihypertensive chronotherapy.
A Wholistic Approach to Non-Pharmacological Intervention for Primary Dysmenorrhea
Authors: Wal Pranay , Gupta Divyanshi , Wal Ankita , Pandey Shiv Shankar , Krishnan Karthickeyan 
Published: January 2023

Background:Primary dysmenorrhea is a prevalent complaint among women nowadays. Primary dysmenorrhea is a painful disorder that occurs during the menstrual cycle. It is described as unpleasant menstrual cramps or period discomfort that occurs without the presence of any pelvic illness. Primary dysmenorrhea is one of the most common causes of absence from school, work, and social activities. It has an impact on women's daily quality of life.Objective:To investigate the efficacy and safety of non-pharmacological treatments for women suffering from primary dysmenorrhea.Methods:We looked up information on gynaecology in the Cochrane library, PubMed, ScienceDirect, and other databases. We looked at multiple clinical trial databases from the year before as well as systematic reviews that had been done before.Result:As per qualitative assessment, non-pharmacological management for primary dysmenorrhea is extremely effective and wonderful for overall health. Primary dysmenorrhea can be treated with any of the approaches outlined in this study.Conclusion:The study revealed that non-pharmacological approaches, such as yoga, physical activity, heat treatment, massage therapy, water therapy, dietary supplements, acupressure, aromatherapy, massage therapy, and other non-invasive techniques, have a significant role in the management of dysmenorrhoea. It was observed that women who engage in regular physical activity do not experience severe dysmenorrhea. Additionally, it was discovered in this study that dietary nutrients like vitamin D and other nutraceuticals ameliorate the symptoms of dysmenorrhea.
Effects of SIRT1 on Osteogenic Differentiation of Bone Marrow Mesenchymal
Stem Cells in Type 2 Diabetic Patients
Authors: Deng Xiangqun , Deng Ling , Xu Min , Sun Yanlei , Yang Mei 
Published: January 2023

Background:
Patients with type 2 diabetes mellitus (T2DM) are at high risk for osteoporosis.
SIRT1 plays an important regulatory role in the occurrence and development of diabetes
mellitus; however, it is still not clear whether SIRT1 is directly related to the osteogenic ability of
bone marrow mesenchymal stem cells (BMSCs) in T2DM patients.

Methods:
We obtained BMSCs from patients with T2DM and healthy volunteers to determine the
effect of SIRT1 expression on the osteogenic capacity of BMSCs. As a result, SIRT1 expression in
BMSCs in T2DM was significantly lower compared to healthy volunteers, but the proliferative capacity
of BMSCs in the T2DM group was not significantly different from that of healthy volunteers.

Results:
During osteogenic differentiation, the expression of SIRT1 in MSCs from T2DM patients
was significantly decreased, and the osteogenic differentiation ability of MSCs from T2DM patients
was significantly lower than healthy volunteers. After intervention with resveratrol, the expression
of SIRT1 increased significantly, and the apoptotic rate of MSCs in T2DM patients decreased significantly.
Moreover, resveratrol promoted osteoblast differentiation of MSCs.

Conclusion:
Our study confirmed that the expression of SIRT1 is directly related to the osteogenic
potential of BMSCs in patients with T2DM. Resveratrol promoted the osteogenic differentiation of
BMSCs by increasing the expression of SIRT1. The increased expression of SIRT1 significantly
reduced BMSC apoptosis during osteogenic differentiation, which is one of the important mechanisms
by which SIRT1 regulates the osteogenic ability of BMSCs. Our data also provide strong
evidence that resveratrol may be used in the treatment of osteoporosis in patients with T2DM.
Control of Bacterial Wilt (Ralstonia solanacearum) and Reduction of Ginger Yield Loss through Integrated Management Methods in Southwestern Ethiopia
Authors: Benti Eyob Aysanew 
Published: January 2023

Background:
Bacterial wilt incited by Ralstonia solanacearum is the most important disease affecting ginger production in southwestern Ethiopia. Unavailability of disease-free planting materials, resistant cultivars, and effective chemical compounds are the key constraints in managing the disease.

Objective:
The study was initiated to determine the effect of integrated management methods on bacterial wilt disease and yield loss of ginger through combining hot water, bio-fumigation, soil-solarization and chemical pesticides.

Methods:
A total of seven treatment combinations comprising hot water, bio-fumigation, soil-solarization, Mancozeb, and bleaching powder were tested in a randomized complete block design in three replications. Data on disease incidence, growth, yield, and yield components were recorded from randomly selected plants.

Results:
The use of Mancozeb for seed socking and soil drenching combined with bio-fumigation and soil-solarization reduced the incidence of bacterial wilt by 63.3% and enhanced the rhizome yield by 66.8%. Rhizome and soil treatment using bleaching powder along with soil bio-fumigation also reduced the disease incidence by 38.9% and increased ginger yield by 61.5%. It also provided the highest (6678.7%) marginal rate of return of any treatment combination tested in the experiment. Disease incidence was highly significantly and inversely (r= -0.98**) correlated with rhizome yield. The regression slope estimated that 83.4% of ginger yield loss was associated with the bacterial wilt disease.

Conclusion:
A combined application of Mancozeb, bio-fumigation and soil-solarization can be used to control ginger bacterial wilt. Alternatively, bleaching powder for rhizome and soil treatment in conjunction with bio-fumigation can be employed as an integrated management system against the disease.