bluuebunny/crossref_metadata_2025_split huggingface dataset:
Qualitative exploration of the visual function impairments and impacts on vision-dependent activities of daily living in Retinitis Pigmentosa and Leber Congenital Amaurosis: content validation of the ViSIO-PRO and ViSIO-ObsRO measures
Authors: Kay Christine , Audo Isabelle , Naujoks Christel , Spera Claudio , Fischer M. Dominik , Green Jane , Durham Todd , Williamson Nicola , Bradley Helena , Barclay Melissa , Sims Joel , Banhazi Judit , Patalano Francesco 
Published: July 2023

Abstract
Background
Retinitis Pigmentosa (RP) and Leber Congenital Amaurosis (LCA) are rare inherited retinal degenerative disorders. The associated visual impairments have significant impacts on patients’ vision-dependent activities of daily living (ADL), mobility, and distal health-related quality of life (HRQoL). To adequately capture patient and caregiver perspectives in clinical trials, patient and observer-reported outcome instruments must demonstrate sufficient evidence of content validity in the target population. This study aimed to explore the patient experience of RP/LCA and assess the content validity of the Visual Symptom and Impact Outcomes PRO (ViSIO-PRO) and ObsRO (ViSIO-ObsRO) instruments in RP/LCA.

Methods
A total of 66 qualitative, combined concept elicitation (CE) and cognitive debriefing (CD) interviews were conducted (33 adults, 10 adolescents, 8 children and 15 caregivers of children) in the US, France, Germany, and Canada. Patients had a clinical and genetic diagnosis of RP/LCA from a range of genotypes. CE results were used to further inform the development of a conceptual model and CD interviews assessed the relevance and understanding of the 44-item ViSIO-PRO and 26-item ViSIO-ObsRO instruments. Interviews were conducted across two iterative rounds to allow item modifications.

Results
Findings were consistent across RP/LCA genotypes. Night blindness, reduced peripheral vision, vision in very bright lighting and light/dark adaptation were the most frequently reported visual function symptoms impacting vision-dependent ADL and mobility. Impacts on distal HRQoL domains were also reported. The ViSIO-PRO and ObsRO items were well understood by participants and relevant across genotypes. The instructions, 7-day recall period and response scales were well understood and endorsed. Participant and expert clinician feedback supported modifications to item wording, the addition of six new ViSIO-PRO items and one new ViSIO-ObsRO item, and the removal of one ViSIO-PRO item due to lack of relevance.

Conclusions
Findings support the content validity of the ViSIO-PRO and ViSIO-ObsRO instruments for use across RP/LCA genotypes. Ongoing research to evaluate the psychometric validity of the instruments will support future use of the instruments as efficacy endpoints in clinical trials and in general clinical practice to track disease severity and impact of disease on functioning.
Extraction of biosurfactant from pseudomonas aeruginosa inhabiting oil-spilled soils
Authors: Yagoo Alex , Vilvest Jelin 
Published: July 2023

Abstract
Background
Screening for biosurfactant-producing microbes involves the process of sampling and isolating bacteria from various environmental sources. Hydrocarbon-contaminated locations are considered ideal sources for isolating bacteria that produce biosurfactants. This is because these microbes have adapted to living in oil-contaminated environments and have developed mechanisms to produce biosurfactants as a means of survival. Therefore, screening microbes from such locations increases the likelihood of finding bacteria capable of producing biosurfactants.

Results
This investigation aimed to isolate bisurfactant-producing bacteria from oil-contaminated soil samples, with a particular focus on Pseudomonas aeruginosa. Out of the 10 samples collected, four were confirmed to be P. aeruginosa, and all strains showed positive results for antagonistic, hemolytic, and emulsification tests. The strains were effective against B. subtilis, S. aureus, and E. coli but less effective against A. hydrophila. The PS10 strain exhibited the highest emulsification index, while PS08 showed the lowest. Based on microscopic and biochemical examination, P. aeruginosa was identified as the most effective isolate.

Conclusion
These findings highlight the potential of P. aeruginosa as a biosurfactant producer for industrial and environmental applications. However, further studies are needed to fully understand the biosurfactant production potential of these strains and to optimize the conditions for biosurfactant production.
Longitudinal Monitoring of Circulating Tumor DNA to Assess the Efficacy of Immune Checkpoint Inhibitors in Patients With Advanced Genitourinary Malignancies
Authors: Jang Albert , Lanka Sree M. , Jaeger Ellen B. , Lieberman Alexandra , Huang Minqi , Sartor A. Oliver , Mendiratta Prateek , Brown Jason R. , Garcia Jorge A. , Farmer Tiffany , Sudhaman Sumedha , Mahmood Tamara , Pajak Natalia , Calhoun Mark , Dutta Punashi , ElNaggar Adam , Liu Minetta C. , Barata Pedro C. 
Published: July 2023

PURPOSE Circulating tumor DNA (ctDNA) detection in blood has emerged as a prognostic and predictive biomarker demonstrating improved assessment of treatment response in patients receiving immune checkpoint inhibitors (ICIs). Here, we performed a pilot study to support the role of ctDNA for longitudinal treatment response monitoring in patients with advanced genitourinary (GU) malignancies receiving ICIs. MATERIALS AND METHODS Patients with histologically confirmed advanced GU malignancies were prospectively enrolled. All eligible patients received ICI treatment for at least 12 weeks, followed by serial collection of blood samples every 6-8 weeks and conventional scans approximately every 12 weeks until disease progression. ctDNA analysis was performed using Signatera, a tumor-informed multiplex-polymerase chain reaction next-generation sequencing assay. Overall, the objective response rate (ORR) was reported and its association with ctDNA status was evaluated. Concordance rate between ctDNA dynamics and conventional imaging was also assessed. RESULTS ctDNA analysis was performed on 98 banked plasma samples from 20 patients (15 renal, four urothelial, and one prostate). The median follow-up from the time of initiation of ICI to progressive disease (PD) or data cutoff was 67.7 weeks (range, 19.6-169.6). The ORR was 70% (14/20). Eight patients ultimately developed PD. The overall concordance between ctDNA dynamics and radiographic response was observed in 83% (15/18) of patients. Among the three patients with discordant results, two developed CNS metastases and one progressed with extracranial systemic disease while ctDNA remained undetectable. CONCLUSION In this pilot study, longitudinal ctDNA analysis for monitoring response to ICI in patients with advanced GU tumors was feasible. Larger prospective studies are warranted to validate the utility of ctDNA as an ICI response monitoring tool in patients with advanced GU malignancies.
Age-related Changes in the Adrenal Cortex: Insights and Implications
Authors: Warde Kate M , Smith Lorenzo J , Basham Kaitlin J 
Published: July 2023

Abstract
Aging is characterized by a gradual decline in physiological function. This process affects all organs including the adrenal cortex, which normally functions to produce essential steroid hormones including mineralocorticoids, glucocorticoids, and androgens. With increasing age, features such as reduced adrenal cortex size, altered zonation, and increased myeloid immune cell infiltration substantially alter the structure and function of the adrenal cortex. Many of these hallmark features of adrenal cortex aging occur both in males and females, yet are more enhanced in males. Hormonally, a substantial reduction in adrenal androgens is a key feature of aging, which is accompanied by modest changes in aldosterone and cortisol. These hormonal changes are associated with various pathological consequences including impaired immune responses, decreased bone health, and accelerated age-related diseases. One of the most notable changes with adrenal aging is the increased incidence of adrenal tumors, which is sex dimorphic with a higher prevalence in females. Increased adrenal tumorigenesis with age is likely driven by both an increase in genetic mutations as well as remodeling of the tissue microenvironment. Novel antiaging strategies offer a promising avenue to mitigate adrenal aging and alleviate age-associated pathologies, including adrenal tumors.
Immediate and longer-term impacts of fetal surveillance education on workforce knowledge and cognitive skills
Authors: Beaves Mark , Zoanetti Nathan , Wallace Euan M , Palmer Kirsten R 
Published: July 2023

Background: Following the development of the Royal Australian College of Obstetricians and Gynaecologists Intrapartum Fetal Surveillance Guideline in 2003, an education program was developed to support guideline implementation and clinical practice. It was intended that improved clinician knowledge, particularly of cardiotocography, would reduce rates of intrapartum fetal morbidity and mortality. The program contains a multiple-choice assessment, designed to assess fetal surveillance knowledge and the application of that knowledge. We used the results of this assessment over time to evaluate the impact of the education program on clinicians’ fetal surveillance knowledge and interpretive skills, in the immediate and longer-term. Methods: We undertook a retrospective analysis of the assessment results for all participants in the Fetal Surveillance Education Program, between 2004 and 2018. Classical Test Theory and Rasch Item Response Theory analysis were used to evaluate the statistical reliability and quality of the assessment, and the measurement invariance or stability of the assessments over time. Clinicians’ assessment scores were then reviewed by craft group and previous exposure to the program. Results: The results from 64,430, broadly similar assessments, showed that participation in the education program was associated with an immediate improvement in clinician performance in the assessment. Performance improvement was sustained for up to 18 months following participation in the program and recurrent participation was associated with progressive improvements. These trends were observed for all craft groups (consultant obstetricians, doctors in training, general practitioners, midwives, student midwives). Conclusions: These findings suggest that the Fetal Surveillance Education Program has improved clinician knowledge and the associated cognitive skills over time. The stable difficulty of the assessment tool means any improvement in clinician’s results, with ongoing exposure to the program, can be reliably assessed and demonstrated. Importantly this holds true for all craft groups involved in intrapartum care and the interpretation of cardiotocography.
Navigating boundaries in coproduced research: a situational analysis of researchers’ experiences within integrated knowledge translation projects
Authors: Ackerley Chris , Balka Ellen 
Published: July 2023

Background:
Research coproduction is advocated as an approach to produce more impactful evidence, by valuing a diversity of expertise and integrating knowledge users into research processes. Yet, extant literature finds that trying to bridge boundaries between different types of knowledge can also cause collaboration challenges and present barriers to success in coproduction.
Aims and objectives:
To study how researchers understand and manage knowledge boundaries in coproduced health research, or ‘integrated knowledge translation’ (IKT) as it is referred to in Canada.
Methods:
Data were collected from: 1) semi-structured interviews (n=20) with researchers leading different IKT projects across Canada; and 2) participant observation and document analysis for an in-depth case study of one IKT project. Data were combined and analysed using situational analysis, a modified grounded theory approach to visually map patterns of discourse along salient axes of controversy.
Findings:
We describe four key discursive positions participants take concerning knowledge boundaries in IKT: to recognise and handle, respect and clarify, blur and integrate, or challenge and embrace. These are plotted relative to two salient axes: the degree to which participants viewed boundaries as a problem, and the degree to which they believed boundaries should (or could) be challenged.
Discussion and conclusion:
The four discursive positions identified will help those doing coproduced research to critically reflect on their own position(s) regarding boundaries in collaborative research, and strategically discuss, select, or switch discourses as needed to support their goals.
Instruments of Power. The affective power of sound design in American music education
Authors: Karvelis Noah , Popkewitz Thomas S. 
Published: July 2023

Music education in the United States has increasingly focused upon student creativity and practices such as improvisation. While such practices and changes are often conceptualized as flexible, inclusive reforms about making kinds of children, historically the curriculum has little to do with music and is instead concerned with taming uncertainty in the governing of child. We examine the Orff-Schulwerk pedagogy in the teaching of music, thought of as a progressive reform which emphasized improvisation and creativity. Exploring briefly Orff-Schulwerk’s production in the Weimar Republic as it travels and translated into the post-war United States, we consider the curriculum as formed through psychological research about rationality, choice, and creativity. The focus is on the system of reasoning embodied in the curriculum as generating principles about desired kind of persons in a stable, secure future related to salvation narratives- principles different in the Weimar Republic and US. The shifting design of people in Orff-Schulwerk curriculum and classroom instruments for learning of sound are, we argue, practices in governing of the body and soul. The fabricating and mapping in the music curriculum is about kinds of populations required as evidence of the progressive desires that simultaneously distributes differences.
Being and the Philosopher’s Object in Plato’s Sophist
Authors: Granieri Roberto 
Published: July 2023

Abstract
In the Sophist, Plato claims that the philosopher is always engaged through reasonings with the idea tou ontos (254a4–b1). I argue that, contrary to appearances and to what various commentators believe, this phrase does not refer to the Kind Being singled out in the Sophist as one of the so-called ‘greatest’ or ‘most important’ Kinds, but to the whole intelligible realm. The proposed reading better accounts for Plato’s exact wording at Sophist 254a4–b1 and preserves the consistency of Plato’s view on the object of philosophical knowledge. I conclude with some broader considerations on the relationship between this passage, Platonic dialectic and Aristotle’s conception of a science of being qua being.
Ritka vércsoport specifikus autoantitest szolid tumoros betegben: auto-anti-N, a direkt Coombs-teszt értelmezése
Authors: Csernus Zita , Cserne Molnár Kinga , Biró Katalin , Scheibl Éva , Dezsőné Siba Krisztina 
Published: July 2023

A vércsoport-szerológiai kompatibilitási vizsgálatsor egyik kötelező eleme a direkt Coombs-teszt, mely a vörösvérsejtek antitesttel vagy komplementtel való fedettségét vizsgálja. A teszt pozitív eredménye sok kérdést vet fel a diagnózis megállapításakor, és útmutató lehet a megfelelő terápia kiválasztásához. A vörösvérsejteken lévő autoantitestek ritkán vércsoport-specifikusak, melyek az ABO vércsoportvizsgálatot is befolyásolhatják, továbbá transzfúziós javaslatnál történő figyelembevételük a beadott vörösvérsejtek hosszabb túlélését biztosíthatják.Több esetben közöltek szolid tumoros betegekben is vörösvérsejt-ellenes, főleg pánreaktív autoantitest-képződést és immunhemolízist. A bemutatott esetünkben ritka IgG auto-anti-N antitest volt azonosítható még nem diagnosztizált vesetumoros beteg szerológiai vizsgálata során, melyet a standard kompatibilitási vizsgálatokon (ABO, RhD, indirekt és direkt Coombs) felül elúciós vizsgálat igazolt ABO vércsoport diszkrepanciával.A beteg laboratóriumi leletei nem igazoltak hemolízist, hidegagglutinin vizsgálata felvételkor negatív volt. Műtéthez ABO, RhD, Kell és N negatív választott vért adtunk, a transzfúzió eseménytelenül zajlott. Az M, N, S és s antigének az MNS vércsoportrendszer transzfúziós szempontból legfontosabb tagjai. A rendszer antigénjei a vörösvérsejt membrán glikoforinok (GYP) szialoglikoproteinjei. Kódoló génjei a GYPA, GYPB és GYPE. A vörösvérsejt membrán glikoforinok citokinek, baktériumok és vírusok receptorai lehetnek. Az MNS antigének a vese endotéliumon és epitéliumon is jelen vannak. Ismert az N antigén és prekurzorainak carcinoma asszociációja.A rendszer alloantitestjei általában hidegben reagáló, transzfúziós szempontból általában nem veszélyes IgM típusú természetes antitestek. A ritkán előforduló IgG antitestek azonban hemolitikus transzfúziós reakciót, újszülöttkori hemolitikus betegséget és autoimmun hemolitikus anémiát okozhatnak.Esetünkkel szeretnénk felhívni a figyelmet a vércsoport-diszkrepancia és a bemutatott ritka autoantitest diagnosztikus értékére, a direkt Coombs-teszt helyes értelmezésének fontosságára, továbbá a szerológiai vizsgálatok körültekintő elvégzésének és értékelésének jelentőségére.
Saúde Coletiva e suas aproximações com a Terapia Ocupacional: reflexões a partir de uma revisão bibliográfica
Authors: Mei Andre Eduardo , Yoneko Dakuzaku Carretta Regina , Filice de Barros Nelson 
Published: July 2023

Introdução: A Saúde Coletiva é um campo de conhecimentos que se destaca pela sua transformação através da inclusão contínua de disciplinas oriundas de diferentes campos científicos, sendo assim irredutível a um paradigma monodisciplinar. Objetivos: Este estudo buscou explorar possíveis afinidades eletivas entre a terapia ocupacional e a saúde coletiva. Método: Foi realizada uma revisão de escopo na base de dados da Biblioteca Virtual em Saúde, com os termos “terapia ocupacional”, “saúde coletiva”, “saúde pública” e “saúde comunitária”. Resultados: Sete artigos foram selecionados para a revisão, que tematizaram o universo da gestão, a prevenção de doenças e agravos, a promoção da saúde, o contexto comunitário de práticas, saúde populacional e a dimensão ocupacional do cuidado. Ademais, o estudo pôde perceber nuances sobre conceitos chave e suas definições distintas encontradas na literatura. Conclusão: O presente artigo pode alimentar o debate acerca das afinidades eletivas entre terapia ocupacional e saúde coletiva, bem como embasar o conhecimento de terapia ocupacional na saúde coletiva.
An examination of teacher collaboration in professional learning communities and collaborative teaching practices
Authors: Khasawneh Yusra Jadallah Abed , Alsarayreh Raghad , Ajlouni Adnan Ahmad Al , Eyadat Haitham Mustafa , Ayasrah Mohammad Nayef , Khasawneh Mohamad Ahmad Saleem 
Published: July 2023

The present study aims to examine the influence of teacher collaboration within professional learning communities (PLCs) and collaborative teaching practices on both the professional growth and academic achievement of students. The study employed a mixed-methods research design that incorporated both qualitative and quantitative research methodologies. This research comprises both survey responses and interview transcripts obtained from K-12 educators who are currently engaged in Professional Learning Communities (PLCs) or collaborative teaching methodologies. The findings of this study have the potential to enhance the existing literature on teacher collaboration and furnish educators, policymakers, and researchers with valuable perspectives to steer optimal strategies for promoting effective teacher collaboration in Professional Learning Communities (PLCs) and collaborative teaching approaches. The objective of the research is to investigate the influence of teacher collaboration within Professional Learning Communities (PLCs) and collaborative teaching practices on the development of professional skills and academic achievements of students. The results of this study underscore the importance of recognizing the advantageous effects that collaborative teaching methodologies can have on the academic achievements of students. This necessitates the provision of adequate time for collaborative activities, encouraging supportive leadership within educational institutions, and allocating sufficient resources for collaboration.
The polymyxin SPR206 remediates gram-negative infections in vivo
Authors: Fitzgerald Timothy , Castellanos Giovanna , Escatte Mariel , Hur Stephen , Majernik Stephanie , Wong Ting , Alamneh Yonas , Abu-Taleb Rania , Su Wanwen , Czintos Christine , Raynor Malik , Chattagul Supaksorn , Lanteri Charlotte A. , Pucci Michael , Getnet Derese , Swierczewski Brett , Kane Brian , Breton Yoann Le , Lister Troy , Zurawski Daniel 
Published: July 2023

Abstract
ESKAPEE pathogens (Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp., and Escherichia coli), particularly the gram-negative species, are a problematic group of bacteria associated with nosocomial infections in susceptible patients. These infections are often further complicated by multidrug-resistant (MDR) strains and a lack of treatment options. While the pipeline of new antibiotics is nothing short of thin when it comes to gram-negative activity, one promising molecule is SPR206. SPR206 is a novel polymyxin antibiotic, a nonapeptide designed to overcome the nephrotoxicity associated with other members of this drug class. As intended, SPR206 was found to be safe with no severe adverse events in a Phase 1, single ascending dose/multiple ascending dose clinical trial. SPR206 has in vitro activity against almost all gram-negative species, and we confirmed this activity in minimal inhibitory concentration assays against unique diversity sets of bacterial isolates. Additionally, bactericidal activity was observed in time-kill assays, which is expected for polymyxins. Subsequently, we evaluated the safety and efficacy of SPR206 in three separate in vivo models: Galleria mellonella and two murine models, a pulmonary model and a wound model. In G. mellonella and in the mouse pulmonary model, SPR206 provided almost 100% survival in treated animal groups when compared to untreated control groups. With wound infection, SPR206 significantly reduced bacterial burden and decreased time to heal compared to untreated groups. With wound infection, SPR206 significantly reduced bacterial burden and decreased time to heal compared to untreated groups. These results suggest SPR206 is a potent antibiotic with MDR gram-negative activity that could have expanded clinical indications to include skin and soft tissue infection and hospital-acquired pneumonia.