bluuebunny/crossref_metadata_2025_split huggingface dataset:
An inducible model for genetic manipulation and fate-tracing of PDGFRβ-expressing fibrogenic cells in the liver
Authors: Hamberger Florian , Mederacke Young-Seon , Mederacke Ingmar 
Published: May 2023

AbstractMyofibroblasts are the source of extracellular matrix protein during liver fibrogenesis. Fibroblasts, hepatic stellate cells (HSCs) and vascular smooth muscle cells are mesenchymal subpopulations in the liver that are characterized by the expression of PDGFRβ and contribute to the pool of these myofibroblasts. Conditional knockout models are important to better understand the function of specific liver cell populations including mesenchymal cells. While there is a limited number of constitutive mouse models for liver mesenchymal cell specific transgene expression, there is no established model for inducible gene targeting in HSCs or PDGFRβ-expressing mesenchymal cell populations in the liver. To address this, we investigated whether the tamoxifen inducible PDGFRβ-P2A-CreERT2 mouse can be used as a reliable tool to specifically express transgens in liver mesenchymal cells. Our data demonstrate, that PDGFRβ-P2A-CreERT2 specifically and efficiently marks over 90% of retinoid positive HSCs in healthy and fibrotic liver in mice upon tamoxifen injection, and that those cells give rise to Col1a1-expressing myofibroblasts in different models of liver fibrosis. Together with a negligible background recombination of only about 0.33%, this confirms that the PDGFRβ-P2A-CreERT2 mouse is nearly as efficient as established constitutive LratCre and PDGFRβ-Cre mouse models for recombination in HSCs, and that it is a powerful model for mesenchymal liver cell studies that require an inducible Cre approach.
Comparison of biomarkers of exposure among US adult smokers, users of electronic nicotine delivery systems, dual users and nonusers, 2018–2019
Authors: Holt Nathan M. , Shiffman Saul , Black Ryan A. , Goldenson Nicholas I. , Sembower Mark A. , Oldham Michael J. 
Published: May 2023

AbstractThe harm caused by cigarette smoking is overwhelmingly due to byproducts of tobacco combustion. Electronic Nicotine Delivery Systems (ENDS) provide nicotine to users without combustion, and may support tobacco harm reduction among cigarette smokers who would not otherwise quit in the near term. Analyses of Wave 5 of the Population Assessment of Tobacco and Health (PATH) Study compared biomarkers of exposure (BOE) levels for nicotine, 3 metals, 2 tobacco-specific nitrosamines and 14 smoking-related volatile organic compounds in 151 exclusive ENDS users, 1341 exclusive cigarette smokers, 115 dual users (cigarettes and ENDS), and 1846 past 30-day nonusers of tobacco, adjusting for demographics. Nicotine exposure in ENDS users and dual users did not significantly differ from smokers. Among ENDS users, 16 of 18 other BOEs were significantly lower than smokers’; 9 BOEs were not significantly different from nonusers. Among dual users smoking < 10 cigarettes/day, 15 of 18 non-nicotine BOEs were significantly lower than smokers’, whereas in dual users smoking ≥ 10 cigarettes per day none of the BOEs significantly differed from smokers’. In this representative sample of US adults, exclusive use of ENDS (vs. cigarette smoking) was associated with much lower exposures to many harmful chemicals associated with smoking-related disease. BOE levels in dual users were directly related to their cigarette consumption. These BOE data provide further evidence that ENDS expose users to substantially lower levels of toxicants than combustible cigarettes, confirming their potential for harm reduction.
Succinylation-associated lncRNA signature to predict the prognosis of colon cancer based on integrative bioinformatics analysis
Authors: Zhang Si-ming , Shen Cheng , Gu Jue , Li Jing , Jiang Xiaohui , Wu Zhijun , Shen Aiguo 
Published: May 2023

AbstractColon cancer (CC) has a poor 5-year survival rate though the treatment techniques and strategies have been improved. Succinylation and long noncoding RNAs (lncRNAs) have prognostic value for CC patients. We analyzed and obtained succinylation-related lncRNA by co-expression in CC. A novel succinylation-related lncRNA model was developed by univariate and Least absolute shrinkage and selection operator (Lasso) regression analysis and we used principal component analysis (PCA), functional enrichment annotation, tumor immune environment, drug sensitivity and nomogram to verify the model, respectively. Six succinylation-related lncRNAs in our model were finally confirmed to distinguish the survival status of CC and showed statistically significant differences in training set, testing set, and entire set. The prognosis of with this model was associated with age, gender, M0 stage, N2 stage, T3 + T4 stage and Stage III + IV. The high-risk group showed a higher mutation rate than the low-risk group. We constructed a model to predict overall survival for 1-, 3-, and 5-year with AUCs of 0.694, 0.729, and 0.802, respectively. The high-risk group was sensitive to Cisplatin and Temozolomide compounds. Our study provided novel insights into the value of the succinylation-related lncRNA signature as a predictor of prognosis, which had high clinical application value in the future.
Weight-loss behaviors before pregnancy associate with increased risk of postpartum depression from the Japan Environment and Children’s Study
Authors: Taniguchi Saki , Masumoto Toshio , Kurozawa Youichi 
Published: May 2023

AbstractNo studies showed the association between weight-loss behaviors before pregnancy and postpartum depression (PPD). We analyzed data from the nation-wide birth cohort study, the Japan Environment and Children’s Study. Self-administrated questionnaires answered by 62,446 women was analyzed using logistic regression analysis. PPD was assessed using the Edinburgh Postnatal Depression Scale at 1 month after delivery. Women using at least one weight-loss method had higher risk of PPD than women using no weight-loss methods [women without antenatal psychological distress according to Kessler 6-Item Psychological Distress score: adjusted odds ratio (aOR) 1.318, 95% confidence interval (CI) 1.246–1.394; women with antenatal psychological distress: aOR 1.250, 95% CI 0.999–1.565]. Using extremely unhealthy weight-loss methods was associated with PPD compared with no use of each weight-loss method (vomiting after eating: aOR 1.743, 95% CI 1.465–2.065; smoking: aOR 1.432, 95% CI 1.287–1.591; taking diet pills: aOR 1.308, 95% CI 1.122–1.520). The association between weight-loss behaviors and PPD varied according to pre-pregnancy BMI. In normal-weight women, the weight-loss method score, which indicates the degree of weight-loss method use, was associated with PPD. These results indicate using weight-loss methods before pregnancy is associated with an increased risk of PPD in Japanese women.
Use of machine learning to assess the prognostic utility of radiomic features for in-hospital COVID-19 mortality
Authors: Sun Yuming , Salerno Stephen , He Xinwei , Pan Ziyang , Yang Eileen , Sujimongkol Chinakorn , Song Jiyeon , Wang Xinan , Han Peisong , Kang Jian , Sjoding Michael W. , Jolly Shruti , Christiani David C. , Li Yi 
Published: May 2023

AbstractAs portable chest X-rays are an efficient means of triaging emergent cases, their use has raised the question as to whether imaging carries additional prognostic utility for survival among patients with COVID-19. This study assessed the importance of known risk factors on in-hospital mortality and investigated the predictive utility of radiomic texture features using various machine learning approaches. We detected incremental improvements in survival prognostication utilizing texture features derived from emergent chest X-rays, particularly among older patients or those with a higher comorbidity burden. Important features included age, oxygen saturation, blood pressure, and certain comorbid conditions, as well as image features related to the intensity and variability of pixel distribution. Thus, widely available chest X-rays, in conjunction with clinical information, may be predictive of survival outcomes of patients with COVID-19, especially older, sicker patients, and can aid in disease management by providing additional information.
New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples
Authors: Gajda-Walczak Aleksandra , Potęga Agnieszka , Kowalczyk Agata , Sek Slawomir , Zięba Sebastian , Kowalik Artur , Kudelski Andrzej , Nowicka Anna M. 
Published: May 2023

AbstractDespite significant progress in cancer therapy, cancer is still the second cause of mortality in the world. The necessity to make quick therapeutic decisions forces the development of procedures allowing to obtain a reliable result in a quick and unambiguous manner. Currently, detecting predictive mutations, including BRCA1, is the basis for effectively treating advanced breast cancer. Here, we present new insight on gene mutation detection. We propose a cheap BRCA1 mutation detection tests based on the surface plasmon resonance (SPR) or quartz crystal microbalance with energy dissipation (QCM-D) response changes recorded during a hybridization process of an oligonucleotide molecular probe with DNA fragments, with and without the BRCA1 mutation. The changes in the morphology of the formed DNA layer caused by the presence of the mutation were confirmed by atomic force microscopy. The unique property of the developed SPR and QCM tests is really short time of analysis: ca. 6 min for SPR and ca. 25 min for QCM. The proposed tests have been verified on 22 different DNA extracted from blood leukocytes collected from cancer patients: 17 samples from patients with various BRCA1 gene mutation variants including deletion, insertion and missense single-nucleotide and 5 samples from patients without any BRCA1 mutation. Our test is a response to the need of medical diagnostics for a quick, unambiguous test to identify mutations of the BRCA1 gene, including missense single-nucleotide (SNPs).
Microtubule depolymerization contributes to spontaneous neurotransmitter release in vitro
Authors: Velasco Cecilia D. , Santarella-Mellwig Rachel , Schorb Martin , Gao Li , Thorn-Seshold Oliver , Llobet Artur 
Published: May 2023

AbstractMicrotubules are key to multiple neuronal functions involving the transport of organelles, however, their relationship to neurotransmitter release is still unresolved. Here, we show that microtubules present in the presynaptic compartment of cholinergic autaptic synapses are dynamic. To investigate how the balance between microtubule growth and shrinkage affects neurotransmission we induced synchronous microtubule depolymerization by photoactivation of the chemical inhibitor SBTub3. The consequence was an increase in spontaneous neurotransmitter release. An analogous effect was obtained by dialyzing the cytosol with Kif18A, a plus-end-directed kinesin with microtubule depolymerizing activity. Kif18A also inhibited the refilling of the readily releasable pool of synaptic vesicles during high frequency stimulation. The action of Kif18A was associated to one order of magnitude increases in the numbers of exo-endocytic pits and endosomes present in the presynaptic terminal. An enhancement of spontaneous neurotransmitter release was also observed when neurons were dialyzed with stathmin-1, a protein with a widespread presence in the nervous system that induces microtubule depolymerization. Taken together, these results support that microtubules restrict spontaneous neurotransmitter release as well as promote the replenishment of the readily releasable pool of synaptic vesicles.
CapsNet-MHC predicts peptide-MHC class I binding based on capsule neural networks
Authors: Kalemati Mahmood , Darvishi Saeid , Koohi Somayyeh 
Published: May 2023

AbstractThe Major Histocompatibility Complex (MHC) binds to the derived peptides from pathogens to present them to killer T cells on the cell surface. Developing computational methods for accurate, fast, and explainable peptide-MHC binding prediction can facilitate immunotherapies and vaccine development. Various deep learning-based methods rely on separate feature extraction from the peptide and MHC sequences and ignore their pairwise binding information. This paper develops a capsule neural network-based method to efficiently capture the peptide-MHC complex features to predict the peptide-MHC class I binding. Various evaluations confirmed our method outperformance over the alternative methods, while it can provide accurate prediction over less available data. Moreover, for providing precise insights into the results, we explored the essential features that contributed to the prediction. Since the simulation results demonstrated consistency with the experimental studies, we concluded that our method can be utilized for the accurate, rapid, and interpretable peptide-MHC binding prediction to assist biological therapies.
Easy-axis magnetic anisotropy in tetragonally elongated cobalt(ii) complexes beyond the spin-Hamiltonian formalism
Authors: Mičová Romana , Rajnák Cyril , Titiš Ján , Bieńko Alina , Moncoľ Ján , Samoľová Erika , Boča Roman 
Published: May 2023

Complexes under study exhibit field-supported SMR. For 1, the relaxation time at T = 2.0 K is τHF = 20 and 2 ms at the applied field BDC = 0.15 and 0.35 T, respectively. For 2 at T = 2.0 K and BDC = 0.1 T, the relaxation time is τHF = 6 ms.
Pyodine: an open, flexible reduction software for iodine-calibrated precise radial velocities
Authors: Heeren Paul , Tronsgaard René , Grundahl Frank , Reffert Sabine , Quirrenbach Andreas , Pallé Pere L. 
Published: May 2023

Context. Many telescopes use an iodine (I2) absorption cell to measure precise radial velocities (RVs), but their data reduction pipelines are all tailored to their respective instrumental characteristics and not openly accessible.
Aims. For existing and future projects dedicated to measuring precise RVs, we have created an open-source, flexible data reduction software to extract RVs from échelle spectra via the I2 cell method. The software, called pyodine, is completely written in Python and has been built in a modular structure to allow for easy adaptation to different instruments.
Methods. We present the fundamental concepts employed by pyodine, which build on existing I2 reduction codes, and give an overview of the software’s structure. We adapted pyodine to two instruments, Hertzsprung SONG located at Teide Observatory (SONG hereafter) and the Hamilton spectrograph at Lick Observatory (Lick hereafter), and demonstrate the code’s flexibility and its performance on spectra from these facilities.
Results. Both for SONG and Lick data, the pyodine results generally match the RV precision achieved by the dedicated instrument pipelines. Notably, our code reaches a precision of roughly 0.69 m s−1 on a short-term solar time series of SONG spectra, and confirms the planet-induced RV variations of the star HIP 36616 on spectra from SONG and Lick. Using the solar spectra, we also demonstrate the capabilities of our software in extracting velocity time series from single absorption lines. A probable instrumental effect of SONG is still visible in the pyodine RVs, despite being a bit damped as compared to the original results.
Conclusions. With pyodine we prove the feasibility of a highly precise, yet instrument-flexible I2 reduction software, and in the future the code will be part of the dedicated data reduction pipelines for the SONG network and the Waltz telescope project in Heidelberg.
Systematics of planetary ephemeris reference frames inferred from pulsar timing astrometry
Authors: Liu N. , Zhu Z. , Antoniadis J. , Liu J.-C. , Zhang H. 
Published: May 2023

Aims. This study aims to investigate the systematic errors in planetary ephemeris reference frames through pulsar timing observations.
Methods. We used the published data sets from several pulsar timing arrays and performed timing analyses for each pulsar using different planetary ephemerides retrieved from the Jet Propulsion Laboratory’s Development Ephemeris (DE), Ephemeris of Planets and the Moon (EPM), and INPOP (Intégration Numérique Planétaire de l’Observatoire de Paris). Then, we compared the timing solutions and modeled the differences in position and proper motion by vector spherical harmonics of the first degree. The timing solutions were also compared with those determined by very long baseline interferometry (VLBI) astrometry.
Results. The orientation offsets between the latest editions of the DE, EPM, and INPOP series do not exceed 0.4 milliarcseconds (mas), while the relative spins between these ephemerides are less than 5 microarcseconds per year (µasyr−1). We do not detect significant glides in either position or proper motion between these ephemerides. The orientation of the pulsar timing frames deviates from that of the VLBI frame from zero by approximately 0.4 mas when considering the formal uncertainty and possible systematics.
Conclusions. The orientation of current planetary ephemeris frames is as accurate as at least 0.4 mas, and the nonrotation is better than 5 µas yr−1.
Accuracy of acetabular component alignment with surgical guidance systems during hip arthroplasty
Authors: Xu Joshua , Veltman Ewout S. , Chai Yuan , Walter William L. 
Published: May 2023

Background: Navigation in total hip arthroplasty has been shown to improve acetabular positioning and can decrease the incidence of mal-positioned acetabular components. This study aimed to assess two surgical guidance systems by comparing intra-operative measurements of acetabular component inclination and anteversion with a post-operative CT scan. Methods: We prospectively collected intra-operative navigation data from 102 hips receiving conventional THA or hip resurfacing arthroplasty through either a direct anterior or posterior approach. Two guidance systems were used simultaneously: an inertial navigation system (INS) and an optical navigation system (ONS). Acetabular component anteversion and inclination were measured on a post-operative CT. Results: The average age of the patients was 64 years (range: 24–92) and the average BMI was 27 kg/m2 (range 19–38). 52% had hip surgery through an anterior approach. 98% of the INS measurements and 88% of the ONS measurements were within 10° of the CT measurements. The mean (and standard deviation) of the absolute difference between the postoperative CT and the intra-operative measurements for inclination and anteversion were 3.0° (2.8) and 4.5° (3.2) respectively for the ONS, along with 2.1° (2.3) and 2.4° (2.1) respectively for the INS. There was a significantly lower mean absolute difference to CT for the INS when compared to ONS in both anteversions (p < 0.001) and inclination (p = 0.02). Conclusions: We found that both inertial and optical navigation systems allowed for adequate acetabular positioning as measured on postoperative CT, and thus provide reliable intraoperative feedback for optimal acetabular component placement.
Level of Evidence: Therapeutic Level II.
Selbstberichtete Kindesmisshandlungen im Vergleich zwischen Ost- und
Westdeutschland
Authors: Spitzer Carsten , Lübke Laura , Müller Sascha , Knorr Stefanie , Flemming Eva 
Published: May 2023

Zusammenfassung
Ziel der Studie Das sozioökologische Modell betont die Relevanz
politischer, kultureller und ökonomischer Sozialisationseffekte
für die Prävalenz von Kindesmisshandlungen. Diese werden durch
einen Vergleich von Kindesmisshandlungen zwischen ost- und westdeutschen
Probanden, die vor der Wende volljährig wurden, untersucht.
Methodik Eine bezüglich Alter, Geschlechtsverteilung und Einkommen
repräsentative Allgemeinbevölkerungsstichprobe wurde zu
Kindesmisshandlungen befragt.
Ergebnisse Von 507 Probanden gaben 22,5% an, in der DDR
sozialisiert worden zu sein. Diese Studienteilnehmer berichteten signifikant
weniger emotionalen Missbrauch als die 77,5%, die in der BRD
groß geworden sind. Die ost- und westdeutschen Probanden unterschieden
sich in keiner anderen Misshandlungsform.
Schlussfolgerung Unsere Befunde unterstreichen die Bedeutung von
Sozialisations- und Enkulturationseffekten auf das Gedächtnis, die bei
der Interpretation der Ergebnisse zu berücksichtigen sind.
Effects of Exercise Intensity on Cardiometabolic Parameters of
Ovariectomized Obese Mice
Authors: Dos-Santos Adriano , do Nascimento Carvalho Bruno , Da Costa-Santos Nicolas , Mello-Silva Fernanda Queiroz de , Pereira Abel de Assis , Jesus Ney Roberto de , De Angelis Kátia , Irigoyen Maria Claudia , Bernardes Nathalia , Caperuto Erico Chagas , Scapini Katia Bilhar , Sanches Iris Callado 
Published: May 2023

AbstractThe aim of this study was to compare the effects of continuous-moderate vs.
high-intensity interval aerobic training on cardiovascular and metabolic
parameters in ovariectomized high-fat-fed mice. C57BL/6 female
ovariectomized were divided into four groups (n=8): low-fat-fed
sedentary (SLF); high-fat-fed sedentary (SHF); high-fat-fed moderate-intensity
continuous trained (MICT-HF); and high-fat-fed high-intensity interval aerobic
trained (HIIT-HF). The high-fat diet lasted 10 weeks. Ovariectomy was performed
in the fourth week. The exercise training was carried out in the last four weeks
of protocol. Fasting glycemia, oral glucose tolerance, arterial pressure,
baroreflex sensitivity, and cardiovascular autonomic modulation were evaluated.
Moderate-intensity continuous training prevented the increase in arterial
pressure and promoted a reduction in HR at rest, associated with an improvement
in the sympathovagal balance in MICT-HF vs. SHF. The high-intensity interval
training reduced blood glucose and glucose intolerance in HIIT-HF vs. SHF and
MICT-HF. In addition, it improved sympathovagal balance in HIIT-HF vs. SHF.
Moderate-intensity continuous training was more effective in promoting
cardiovascular benefits, while high-intensity interval training was more
effective in promoting metabolic benefits.
Aktivitäten der Sektion Gewebetransplantation und Biotechnologie der Deutschen Ophthalmologischen Gesellschaft: Leistungsbericht 2021
Authors: Reinshagen Helga , Böhringer Daniel , Schroeter Jan , Maier Philip Christian 
Published: May 2023

ZusammenfassungDie Sektion Gewebetransplantation und Biotechnologie der Deutschen Ophthalmologischen Gesellschaft (DOG) legt ihren 11. Leistungsbericht des Jahres 2021 vor. Die Anzahl der eingeworbenen Augenhornhäute ist im Vergleich zu den Vorjahren leicht ansteigend. Dennoch besteht immer noch ein Bedarf für Import aus dem Ausland, somit ist der Transplantatengpass immer noch nicht beseitigt.
Practical Synthesis of Pent-4-yn-1-ol and Pent-4-en-1-ol from Tetrahydrofurfuryl Halides
Authors: Saito Katsuya , Sawazaki Tomoya , Furusawa Osamu , Kosugi Teruo , Kimura Yoshikazu , Kirihara Masayuki 
Published: May 2023

AbstractPent-4-yn-1-ol was prepared in a high yield by the reaction of tetrahydrofurfuryl chloride with n-BuLi in t-BuOMe at 0 °C. Pent-4-en-1-ol was prepared in a high yield by either the reaction of tetrahydrofurfuryl chloride or tetrahydrofurfuryl bromide with lithium in tetrahydrofuran at temperatures from 0 °C to ambient temperature.