bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of the bioactive compounds with efficacy against epilepsy from the herb pairs Polygala tenuifolia - Zizyphus jujuba by modulating CHRNA4/CaMKII signaling pathway: LC-MS/MS combined with network pharmacology analysis and experimental evidence
Authors: Nian Meng , Zhao Zefeng , Wang Yongqi , Chen Jingxuan , Qian Mingcheng , Li Guangning , Li Xiaoan , Qiao Haifa , Yang Xiaohang 
Published: April 2023

Abstract
Background Epilepsy is a typical nervous system disorders identified by the spontaneous recurrence of seizures which injure periods of electroencephalographic activity and behavior. Traditional Chinese Medicine (TCM) herb pairs Polygala tenuifolia and Zizyphus jujuba have been used in treatment of epilepsy in China, while the mechanism of action still remains unclear. This article aims to disclose the substances and potential mechanisms of the anti-epilepsy activity of P. tenuifolia and Z. jujuba extract (PZE) using LC-MS/MS, network pharmacology, ethology and molecular biology methods.Methods With the help of the self-built components database, identification of the chemical parameters of PZE was possessed through LC-MS/MS method, and the “ingredient-target-pathway” network of PZE was established through online databeses. Molecular docking was performed using Discovery Studio Visualizer. In the setting of the epilepsy model, pentylenetetrazol (PTZ, 10 mg/kg) was administered intraperitoneally injected for a period of 21 days. Mice were assessed for anxiety-like behavior by Elevated plus maze test, open field test, forced swimming test and tail suspension test. HE staining, western blotting, and immunofluorescence staining were used to detect morphological changes and signal pathway.Results Through network analysis, 37 active ingredients were obtained from PZE, SLC6A4, CHRNA4 and MAOA and were found to play a major role in the PPI network. GO and KEGG analyses that display their anti-epilepsy activity. The"Ingredient-target-pathway"network diagram consists of 99 targets, 24 kinds of constituents, and 20 signaling pathways. The values of M15 and M17 show the largest degree. Molecular docking analysis shows the key components screened by network pharmacology have a good interaction with the predicted targets. Animal experiments results showed that: 1) PZE effectively lengthened the latent time of PTZ-induced epilepsy in mice model. 2) PTZ-induced depression-like behavior was strikingly ameliorated by PZE. 3) Hippocampal neurons are significantly shielded by PZE. 4) PZE was shown to play a key role in modulating the CHRNA4/CaMK II signaling pathway in to show anti-epilepsy potency.Conclusion This study has successfully identified constituents of PZE through LC-MS/MS methods and predicted the potential targets and CHRNA4/CaMK II as potential signaling pathways of anti-epilepsy effects for PZE, which was proved by animal experiments. The results of this paper are conducive to the systematic elucidating of its mechanism of action and the development of TCM-based anti-epilepsy agents.
Investigation on dynamic plastic deformation behaviors of laser-driving metal sheet impacted by forming die
Authors: Zhang Xingquan , Wang Yinkai , Duan Shiwei , Zhang Yan , Fang Jinxiu , Zuo Lisheng , Hu Wenfeng , Zhang Zhao 
Published: April 2023

Abstract
Laser shock forming (LSF) is an advanced sheet forming technology which has extensive application prospects in micro electronics industry. A two-dimensional axial symmetric numerical model of SUS304 stainless steel is established. Finite element method is used to explore the dynamic deformation behaviors of the sheet driven by intense laser. The results show that the node velocity of metal sheet increases in an oscillatory manner in initial stage and then it starts to bulge. After collision with die cavity bottom, the central region of metal sheet jumps up reversely. Experiencing repeated damped vibration, the mental sheet gradually exhausts the obtained kinetic energy from the shock wave, and the metal sheet acquires the geometrical shape of die cavity. Increasing laser shock pressure can enhance the forming precision of metal sheet. The corresponding experiments are also carried out to verify the predicted results. The applied method and gained results can provide a reference for theoretical research and parameters optimization in LSF.
Kidney-related manifestations or syndromes associated with drugs: a protocol for a database study
Authors: Baptista Alexandre , Marreiros Ana Maria , Macedo Ana , Coelho André 
Published: April 2023

Abstract
Rationale and Background: Drug-related problems are an underdiagnosed and frequently forgotten diagnosis that accounts for a significant cut of medical expenses, morbidity, and mortality. Drug-induced kidney disease is a subsection of such problem and a major contributor to kidney disease which lacks major worldwide investigation.
Objectives: Our main objective is to identify the predominant drugs associated with the main renal manifestations that clinicians deal with daily, allowing clinicians to identify and correctly approach drug-induced kidney disease.
Methods: The authors will collect data from a major worldwide database that collects spontaneous drug-adverse reaction notifications. The statistical analysis will consider four different steps: 1) Univariable descriptive analysis, 2) Inferential analysis for two variables, 3) Classification analysis either with clustering techniques or with models in the format of decision trees, and 4) Interdependence analysis. To perform such statistical tests, IBM SPSS Statistics, version 28.0 and R language will be used.
Discussion: Kidney-related manifestations or syndromes associated with drugs are extremely underdiagnosed but responsible for considerable iatrogenic consequences, either clinically or in costs. The majority of such manifestations are recoverable, and patients can recover with few or no consequences if the diagnosis is made in a timely manner, making the diagnosis of drug-induced kidney disease a highly impactable action. With this study, the authors aim to bring into light new data and information that will increase the recognition and improve the diagnosis of such conditions.
Comprehensive Influence of Ultrasonic Vibration-Assisted Technology on Material Removal Mechanism under the Excitation of Regenerative Vibration in Precision Grinding Process
Authors: chen yong , Chen Xun , Hu Zhongwei , Duan Nian 
Published: April 2023

Abstract
Self-excited regenerative vibration, a well-known endogenous excitation mechanism, has a critical influence on material removal behavior and ground surface generation in a precision grinding process. During ultrasonic vibration-assisted grinding (UVAG), one-dimensional or two-dimensional ultrasonic vibration exerted to abrasive wheel or workpiece in axial and feed directions will influence material removing process significantly. Focusing on the study on the coupling relationship between ultrasonic vibration (as an external forced vibration) and regenerative vibration (as an internal vibration) excited by adjacent active abrasive grits, the improved theoretical models of cutting depth of abrasive grits and phase difference of grinding trajectory paths between adjacent active abrasive grits were presented in the paper with the consideration of the relationships between the ultrasonic vibration frequency, active abrasive grit number in contact zone and abrasive wheel speed. An optimization control strategy was further put forward by means of adjusting ultrasonic vibration frequency and the corresponding ratio to obtain proper phase difference of grinding trajectory paths of abrasive grits, which resulted to the improvement of cutting depth distribution and high surface finish of workpiece. The experiments of UVAG were conducted to verify the reliability of the influential mechanism and the validity of theorical model of cutting depth. Compared with the morphological characteristics of scratch section on the workpiece surface in axial cross-section before and after adjustment of ultrasonic vibration frequency, the results indicated that the numbers of active abrasive grits in contact zone increased with the increase of ultrasonic vibration frequency. When the vibration phase difference between grinding trajectory paths of adjacent abrasive grits was optimized to be an odd multiple of π/2 by stipulating proper ultrasonic vibration frequency, the vibratory amplitude of active abrasive grits decreases. As a result, the cutting depth of abrasive grits and the distribution of workpiece surface waviness were comparatively uniform, and the average surface roughness measured in five groups of experiments decrease by up to 27% in comparing with those without UVAG. In contrast, when the vibration phase difference was an even multiple of π/2, the amplitude of workpiece surface waviness reached to its maximum due to the double excitation effects of regenerative vibration and ultrasonic vibration, the surface roughness worsened by 23%. The finding provides a clear guidance to the preparation of structured abrasive tools and matching strategy of process parameters in UVAG.
ARRB1 Protects Intestinal Tight Junction through Promoting Mitofusin 2 Transcription to Drive Parkin-dependent Mitophagy in Colitis
Authors: Wu Bin , Wu Shuyun , Liu Huiling , Yi Jiazhi , Xu MinYi , Jie Jiang , Tao Jin 
Published: April 2023

Abstract
Intestinal barrier defect is a hallmark of inflammatory bowel disease (IBD). Mitochondrial dysfunction results in energy deficiency and oxidative stress, which contribute to the pathogenesis of IBD. Arrestin beta 1 (ARRB1) is a negative regulator that promotes G protein-coupled receptors (GPCRs)desensitization, endocytosis, and degradation. Our previous study indicated that ARRB1 was involved in mucosal protection in colitis; however, its role in maintaining the intestinal barrier is still unclear. In the present study, we demonstrated that ARRB1 protected the intestinal tight junction barrier against experimental colitis in vivo. ARRB1 deficiency was accompanied by abnormal mitochondrial morphology, lower ATP production, and severe oxidative stress. In vitro, the knockdown of ARRB1 reduced ATP levels and mitochondrial membrane potential while increasing reactive oxygen species levels and oxidative stress. Upon ARRB1 ablation, mitophagy was inhibited, accompanied by decreased LC3BII, phosphatase and tension homologue induced protein kinase1 (PINK1) and parkin, but increased p62 expression. Mitophagy inhibition via PINK1 siRNA or mitochondrial division inhibitor 1 (Mdivi-1) impaired ARRB1-mediated tight junction protection. Mitofusin2 is a critical ubiquitinated substrate for parkin accumulation in mitochondria. Co-immunoprecipitation and luciferase assays indicated that the interaction of ARRB1 with E2F1 activated mitophagy by enhancing the transcription of mitofusin2. Thus, our results suggest that ARRB1 is critical to maintaining the intestinal tight junction barrier by modulating mitophagy. This finding indicates that ARRB1 might be a potential therapeutic target to prevent IBD progression by maintaining mitochondrial homeostasis.
New Clinical Application of Indocyanine Green in Fluorescence- Guided Laparoscopic Lymph-node Biopsy in Case of Lymphoma. Preliminary Results on a Case Series
Authors: Casaccia Marco , Ibatici Adalberto , Ballerini Filippo , Barabino Nicolò Maria , Santoliquido Matteo , Cian Franco 
Published: April 2023

Abstract
Background Indocyanine Green (ICG) fluorescence-guided surgery is widely used for intraoperative visualization of lymphatic structures. To date, there are no reports indicating this dye being used in lymph node biopsies for suspected or relapsed lymphoma.Methods Between October 2021 and June 2022, 12 patients underwent a fluorescence-guided laparoscopic lymph node biopsy (FGLLB) using ICG. The following was retrospectively evaluated: the dosage of ICG, the injection site, the number of patients where fluorescence was obtained after ICG administration, and additionally, the parameters indicating the outcome of the surgical procedure.Results The median duration of the surgery was 90 minutes. A laparotomy conversion was required in one case due to bleeding. Fluorescence was obtained in 10/12 (83.3%) patients by means of subcutaneous/perilesional injection in six of the patients, and intravenously in the other four. Hospitalization had a mean duration of three days. There were no major postoperative complications. FGLLB was used in seven patients to follow lymphoproliferative disease progression, and in five patients to establish a diagnosis. In all cases, FGLLB provided the information necessary for the correct diagnosis.Conclusions Fluorescence with ICG offers a simple and safe method for detecting pathological lymph nodes. FGLLB in suspected intra-abdominal lymphoma can largely benefit from this new opportunity which has not yet been tested to date. Further studies with large case series are needed to confirm its efficacy.
Alteration of gut microbiota in post-stroke depression patients with Helicobacter pylori infection
Authors: Cheng Hong , Dong Siyu , Chen Han , Sun Mei , Zhang Guoxin , Zhou Xiaoying 
Published: April 2023

Abstract
Several studies have identified an association between the gut microbiome and post-stroke depression (PSD). As a common gastric infection bacteria, Helicobacter pylori (H. pylori) infection cause significant alterations in the composition of the gastrointestinal microbiome, but relationship between H. pylori infection and PSD is still limited. Here, we conducted a retrospective study to assess risk factors associated with PSD. A total of 183 patients with ischemic stroke were enrolled and their depression scores, imaging features and clinical data were analyzed. Multivariate logistic regression analysis showed that deep white matter lesions (DWMLs) and H. pylori infection were the independent risk factors for PSD. Further analysis indicated that patients with H. pylori-positive infection [H. pylori (+)] had more severe depressive symptoms than those with negative infection [H. pylori (-)]. Fecal 16S rRNA gene sequencing analysis revealed statistically differences in intestinal flora between H. pylori (+) patients and H. pylori (-) patients. The DESeq2 analysis indicated that Akkermansia muciniphila, Bacteroides dorei, and Fusobacterium ulcerans levels were significantly decreased, while Megamonas funiformis and Bifidobacterium adolescentis were more abundant in the H. pylori (+) group. GC-MS revealed that short-chain fatty acids (SCFAs) concentrations were significantly different between the two groups, and fecal SCFAs concentrations reduced in the H. pylori (+) group. In conclusion, DWMLs and H. pylori infection may play important roles in the development of PSD. H. pylori infection is likely to be involved in the pathogenesis of PSD by altering the intestinal flora.
LC-MS/MS-based Quantification of Tryptophan, Kynurenine, and Kynurenic Acid in Human Placental, Fetal Membranes, and Umbilical Cord Samples
Authors: Pedraz-Petrozzi Bruno , Marszalek-Grabska Marta , Kozub Anna , Szalaj Klaudia , Trzpil Alicja , Stachniuk Anna , Lamadé Eva Kathrin , Gilles Maria , Deuschle Michael , Turski Waldemar A. , Fornal Emilia 
Published: April 2023

Abstract

Tryptophan breakdown metabolites formed along kynurenine pathway play a significant role in pregnancy and fetal development. To understand the involvement of tryptophan (TRP), kynurenine (KYN), and kynurenic acid (KYNA) in these phenomena, it is essential to quantify their levels in relevant biological samples, such as the placenta, fetal membranes, and umbilical cord. In this study, the level of TRP, KYN, and KYNA was determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS method was optimized for high sensitivity and specificity; it showed good reproducibility with a precision of <10% CV and an accuracy of 85-115%. The lower limit of quantification was 0.5 µg/ml for both TRP and KYN and 0.5 ng/mL for KYNA. The method was linear over the examined range of concentrations in homogenate: 0.5 to 30 µg/ml for both TRP and KYN and 0.5 to 25 ng/ml for KYNA. Maternal placenta samples exhibited lower KYNA concentrations but higher KYN concentrations and significantly increased KYN/TRP ratio. However, the umbilical cord had a significantly higher KYNA/KYN ratio than the other two analyzed tissues. No differences were observed in the KYNA/TRP ratio. Collectively, this finding may facilitate further research and provide inside into the involvement of kynurenine pathway of TRP metabolism in fetal development.
Hydrosalpinx: interstitial pregnancy following ipsilateral salpingectomy risk factor? a case-control study with propensity score matching.
Authors: Wu Wei-Fang , Yi Jing-Song , Xie Xi , Liu Chao-Bin 
Published: April 2023

Abstract
To identify risk factors associated with interstitial pregnancy following ipsilateral salpingectomy and discuss possible prevention.A retrospective cohort study was conducted in a single, large, university-affiliated hospital. Data of 29 patients diagnosed with interstitial pregnancy following ipsilateral salpingectomy from January 2011 to November 2020 were assigned into the case group (IP group), whereas 87 patients derived from 6151 patients with intrauterine pregnancy following unilateral salpingectomy were served as the controls using propensity score matching (PSM) at a ratio of 1:3. After PSM, there was no statistically significant difference in patient demographic details between the two groups . The multipara was greater and the intrauterine operation was more frequent in the IP group compared with the control group (P＜0.05). There was only one patient undergoing IVF-ET in the IP group as compared with 29 cases in the control group (3.4% vs. 33.3%, P＜0.05). Salpingectomy was performed on 5 patients in the IP group and 4 patients in the control group due to hydrosalpinx (P＜0.05). Logistic regression indicated that hydrosalpinx was the high risk factor of interstitial pregnancy following ipsilateral salpingectomy (OR=8.175).Our PSM analysis suggests that hydrosalpinx has significantassociation with risk of interstitial pregnancy following ipsilateral salpingectomy in subsequent pregnancy.
Gastrointestinal parasites of exotic avian fauna living in captivity in the state of Bagmati, Kathmandu Valley, Nepal
Authors: chokhal keshab , Bohora Ashok , Gupta Ranjana 
Published: April 2023

Abstract
The research investigated the prevalence of gastrointestinal parasites by analyzing the fecal samples of 100 captive-breed exotic birds from 16 species in the Kathmandu Valley. A total of five different parasite types were found in the study, with a 54% prevalence rate. Helminths were less frequent (33%) than protozoan pathogens (37%) in the samples. Five parasitic genera were found during the examination of the feces, including one protozoa (Eimeria species) and four helminths (Ascaridia species, Trichostrongylus species, Strongyloides species, and Codiostomum species), with Ascaridiaspecies showing the highest prevalence rate (23%) of the helminthes parasites. The bulk of infections were single infections, with mixed infections accounting for the minority. To control parasitic infections, effective hygiene and de-worming initiatives were put in place. A re-infection is still a chance, though; because of things like stress and being near other foraging birds that might be carrying parasites.
Benchmarking Compound Activity Prediction for Real-World Drug Discovery Applications
Authors: Zeng Jianyang , Tian Tingzhong , Li Shuya , Zhang Ziting , Chen Lin , Zou Ziheng , Zhao Dan 
Published: April 2023

Abstract

Identifying active compounds for target proteins is one of the most fundamental objectives in early drug discovery. Recently, a growing number of computational methods, especially data-driven methods, have demonstrated promising potential in predicting compound activities. However, there lacks a well-designed benchmark to objectively evaluate these methods from the aspects of practical applications, which can consider the biased distribution of current real-world compound activity data and adopt appropriate evaluation metrics to avoid overestimation of model performances. In this paper, we propose a novel benchmark, named CARA, to systematically evaluate the performances of compound activity prediction models from a practical perspective. To eliminate the biases in current compound activity data, two types of assays, i.e., virtual screening (VS) and lead optimization (LO), were distinguished according to their drug discovery stages. New train-test splitting schemes and evaluation metrics including the assay-based evaluation and success rates were adopted to comprehensively measure the performances of different methods. Evaluation of several state-of-the-art models on our CARA benchmark demonstrated a more accurate, informative, and direct understanding of model performances than the commonly-used bulk evaluation. In addition, another popular application scenario, in which there were a few already measured task-related samples, was also considered by CARA. Evaluation of a number of few-shot training strategies demonstrated their different preferences for VS and LO tasks, further suggesting the necessity of distinguishing the two task types. A useful indicator was also discovered to estimate the confidence of model predictions on the experimentally unmeasured data. Overall, our CARA benchmark can provide a high-quality dataset for developing and evaluating compound activity prediction models, and the analysis results and new findings in this work can also act as useful guidance for drug discovery applications. New challenges and opportunities were also revealed and discussed for improving current data-driven methods.
Australian brown seaweeds as a source of essential dietary minerals
Authors: Skrzypczyk Vanessa M , Callahan Damien L , Francis David S , Bellgrove Alecia 
Published: April 2023

Abstract

Interest in the potential to both farm and wild-harvest seaweeds in Australia is rapidly increasing. Seaweeds are rich sources of dietary trace minerals and essential elements that are often not found in terrestrial edible plant foods. Due to the high affinity for mineral and contaminant accumulation by seaweeds, it is important to conduct in depth studies on seaweeds that have edible potential and are being considered for sustainable aquaculture or wild harvest. This study screened six Australian fucoid and laminarian brown seaweeds for their dietary mineral composition. Spatial replicate samples of each species were collected from three ‘clean’ Victorian sites (classified based on their proximity to urbanisation and potential pollution sources) and processed with methods consistent for human consumption. Dried and ground samples were analysed by ICP-MS and concentrations of 12 key dietary minerals were compared amongst species. The overall dietary mineral composition differed significantly amongst all species, except between two species in the Sargassaceae; but concentrations of individual dietary minerals varied amongst species in complex ways. Results are discussed in the context of the potential health benefits from consuming the species studied in realistic quantities (e.g. 10 g·d-1 dried seaweed) as part of balanced omnivorous and plant-based diets. This study demonstrated high levels of dietary minerals essential for optimal human health in the Australian brown seaweeds investigated, but further investigation into ways to reduce iodine content in some species may be required to maximise health benefits from regular consumption.