bluuebunny/crossref_metadata_2025_split huggingface dataset:
Premature ovarian insufficiency is associated with global alterations in the regulatory landscape and gene expression in balanced X-autosome translocations
Authors: Di-Battista Adriana , Favilla Bianca Pereira , Zamariolli Malú , Nunes Natália , Defelicibus Alexandre , Armelin-Correa Lucia , da Silva Israel Tojal , Reymond Alexandre , Moyses-Oliveira Mariana , Melaragno Maria Isabel 
Published: May 2023

Abstract
Background
Patients with balanced X-autosome translocations and premature ovarian insufficiency (POI) constitute an interesting paradigm to study the effect of chromosome repositioning. Their breakpoints are clustered within cytobands Xq13–Xq21, 80% of them in Xq21, and usually, no gene disruption can be associated with POI phenotype. As deletions within Xq21 do not cause POI, and since different breakpoints and translocations with different autosomes lead to this same gonadal phenotype, a “position effect” is hypothesized as a possible mechanism underlying POI pathogenesis.

Objective and methods
To study the effect of the balanced X-autosome translocations that result in POI, we fine-mapped the breakpoints in six patients with POI and balanced X-autosome translocations and addressed gene expression and chromatin accessibility changes in four of them.

Results
We observed differential expression in 85 coding genes, associated with protein regulation, multicellular regulation, integrin signaling, and immune response pathways, and 120 differential peaks for the three interrogated histone marks, most of which were mapped in high-activity chromatin state regions. The integrative analysis between transcriptome and chromatin data pointed to 12 peaks mapped less than 2 Mb from 11 differentially expressed genes in genomic regions not related to the patients’ chromosomal rearrangement, suggesting that translocations have broad effects on the chromatin structure.

Conclusion
Since a wide impact on gene regulation was observed in patients, our results observed in this study support the hypothesis of position effect as a pathogenic mechanism for premature ovarian insufficiency associated with X-autosome translocations. This work emphasizes the relevance of chromatin changes in structural variation, since it advances our knowledge of the impact of perturbations in the regulatory landscape within interphase nuclei, resulting in the position effect pathogenicity.
Plant growth regulators improve nitrogen metabolism, yield, and quality of soybean–rhizobia symbiosis
Authors: Chen Wenhao , Li Juan , Yuan Huawei , You Ling , Wei Qin , Feng Ruizhang , Jiang Sipei , Zhao Xiaohong 
Published: May 2023

Abstract
Purpose
In recent years, considerable research has been conducted on the use of plant growth regulators (PGRs) to improve crop yields. Large amounts of PGRs are applied to regulate crop growth. However, few studies have assessed the impact of PGRs, which leave soil residues, on soil microorganisms, especially rhizobia.

Methods
In this study, the influence of inoculation of soybean roots with Sinorhizobium fredii and Bradyrhizobium japonicum exposed to gibberellin A3 (GA3) and mepiquat chloride (MC) on nodule number, nitrogenase activity, and a symbiotic nitrogen fixation gene (fixA) expression was studied. The nitrate content, free amino acids, and nitrate reductase activity of the roots, and seed yield and quality of soybean were measured.

Result
Nodulation was promoted to some extent, whereas nitrogenase activity and fixA expression were inhibited to some extent by treatment with GA3; however, these effects were reversed by treatment with MC. In a pot experiment to study phenotypic characteristics, rhizobia treatment in combination with GA3 or MC significantly improved soybean yield and seed nitrogen content, and increased the root nitrate content, free amino acid content, and nitrate reductase activity.

Conclusion
The results indicated that PGRs, which leave soil residues, have significant positive effects on the growth and activity of soybean and rhizobia.
Vibrio vulnificus pneumonia with multiorgan failure: a case report and review of the literature
Authors: Abeyagunawardena Ishanya , Priyankara Dilshan 
Published: May 2023

Abstract
Background
Vibrio vulnificus is a gram-negative bacterium causing three clinical syndromes namely, gastrointestinal symptoms, skin sepsis and primary sepsis. Primary sepsis exhibits mortality rates exceeding 50%, particularly in the immunocompromised. Vibrio vulnificus is transmitted via consumption of contaminated seafood and contaminated seawater skin exposure. We describe a rare case of an immunocompetent male presenting with an atypical Vibrio vulnificus infection, culminating in severe pneumonia requiring intensive care.

Case presentation
A 46 year old Indian male dockyard worker, a non-smoker and teetotaler, of Indian origin presented to the emergency treatment unit of a tertiary care hospital in Sri Lanka, with fever, productive cough with yellow sputum, pleuritic chest pain and tachypnea for five days. He had no gastrointestinal or skin manifestations. His respiratory rate was 38 breaths/min, pulse rate was 120 bpm, blood pressure was 107/75 mmHg and pulse oximetry was 85% on air. Chest X-ray revealed consolidation of the left lung. Empiric intravenous Piperacillin-tazobactam and Clarithromycin were commenced after obtaining blood and sputum cultures. Over the next 24 h, his oxygen requirement rose and as he required vasopressor support, he was admitted to the intensive care unit. He was intubated and bronchoscopy was performed on day two, which demonstrated thick secretions from left upper bronchial segments. His antibiotics were changed to intravenous ceftriaxone and doxycycline following a positive blood culture report of Vibrio vulnificus. He was ventilated for ten days and his intensive care stay was complicated with a non-oliguric acute kidney injury, with serum creatinine rising up to 8.67 mg/dL (0.81–0.44 mg/dL). He developed mild thrombocytopenia with platelets dropping to 115 × 103 /uL (150–450 × 103/uL) which resolved spontaneously. Vasopressors were weaned off by day eight and the patient was extubated on day ten. He was discharged from intensive care on day twelve and made a full recovery.

Conclusions
Pneumonia itself is an atypical manifestation of Vibrio vulnificus and furthermore, this patient was immunocompetent and did not exhibit the classical gastro-intestinal and skin manifestations. This case highlights the occurrence of atypical Vibrio sp. infections in patients with high exposure risks and the need for early supportive and appropriate antibiotic therapies.
Circuit- and laminar-specific regulation of medial prefrontal neurons by chronic stress
Authors: Liu Wei-Zhu , Wang Chun-Yan , Wang Yu , Cai Mei-Ting , Zhong Wei-Xiang , Liu Tian , Wang Zhi-Hao , Pan Han-Qing , Zhang Wen-Hua , Pan Bing-Xing 
Published: May 2023

Abstract
Background
Chronic stress exposure increases the risk of mental health problems such as anxiety and depression. The medial prefrontal cortex (mPFC) is a hub for controlling stress responses through communicating with multiple limbic structures, including the basolateral amygdala (BLA) and nucleus accumbens (NAc). However, considering the complex topographical organization of the mPFC neurons in different subregions (dmPFC vs. vmPFC) and across multiple layers (Layer II/III vs. Layer V), the exact effects of chronic stress on these distinct mPFC output neurons remain largely unknown.

Results
We first characterized the topographical organization of mPFC neurons projecting to BLA and NAc. Then, by using a typical mouse model of chronic restraint stress (CRS), we investigated the effects of chronic stress on the synaptic activity and intrinsic properties of the two mPFC neuronal populations. Our results showed that there was limited collateralization of the BLA- and NAc-projecting pyramidal neurons, regardless of the subregion or layer they were situated in. CRS significantly reduced the inhibitory synaptic transmission onto the BLA-projecting neurons in dmPFC layer V without any effect on the excitatory synaptic transmission, thus leading to a shift of the excitation-inhibition (E-I) balance toward excitation. However, CRS did not affect the E-I balance in NAc-projecting neurons in any subregions or layers of mPFC. Moreover, CRS also preferentially increased the intrinsic excitability of the BLA-projecting neurons in dmPFC layer V. By contrast, it even caused a decreasing tendency in the excitability of NAc-projecting neurons in vmPFC layer II/III.

Conclusion
Our findings indicate that chronic stress exposure preferentially modulates the activity of the mPFC-BLA circuit in a subregion (dmPFC) and laminar (layer V) -dependent manner.
On the statistical background of quantum mechanics: generalities and a concrete example
Authors: Le Berre Martine , Pomeau Yves 
Published: May 2023

AbstractWe revisit our description of randomness in quantum processes that began in collaboration of Jean Ginibre. The calculations were performed on a worked example: the fluorescence of a single two-level atom pumped by a resonant laser field. This pump laser is described classically (by a function, not an operator). Our aim is first to built a Kolmogorov-type equation (K-equation) for the atomic state, so that the two parameters θ, φ that define this density matrix are random functions of time, therefore the atomic density matrix is a random density matrix. Such an approach, initiated for gas kinetics, was not yet applied to quantum phenomena, whereas it is especially tailored to very quick events well separated (in time) like the quantum jumps observed in spontaneous emission of photons by an atom. Here, we try to clarify the basis of our statistical approach leading to the K-equation below, and we present the main results deduced from it. We explain finally that our approach can be interpreted in terms of Everett’s theory of many-worlds, because at every emission a new history begins for the atom, with two nonoverlapping wave functions.
The influence of maternal reflective functioning and parenting behavior on infant development in the context of perinatal intimate partner violence: a study protocol
Authors: Jongenelen Inês , Pinto Tiago Miguel , Costa Raquel , Pasion Rita , Morais Ana , Henriques Sandra , Lamela Diogo 
Published: May 2023

Abstract
Background
Intimate partner violence (IPV) affects 25% of children under the age of five worldwide, yet the impact of perinatal IPV and its underlying mechanisms on infant development remains poorly understood. IPV indirectly affects infant development through the mother’s parenting behavior, but research on maternal neuro and cognitive processes, such as parental reflective functioning (PRF), is scarce, despite its potential as an unfolding mechanism. The objective of our study, Peri_IPV, is to examine the direct and indirect pathways linking perinatal IPV and infant development. We will analyze the direct impact of perinatal IPV on mothers’ neuro and cognitive parental reflective functioning (PRF) and parenting behavior during the postpartum period, the direct impact of perinatal IPV on infant development, and whether maternal PRF mediates the link between perinatal IPV and parenting behavior. We will also explore the mediation role of parenting behavior in the association between perinatal IPV and infant development and whether the impact of perinatal IPV on infant development occurs through the links between maternal PRF and parenting behavior. Finally, we will examine the moderation role of mother’s adult attachment in the impact of perinatal IPV on maternal neuro and cognitive PRF and parenting behavior during the postpartum period, as well as on infant development.

Methods
Our study will use a multi-method, prospective design to capture different levels of PRF, parenting behavior, and infant development. Three-hundred and forty pregnant women will participate in a 4-wave longitudinal study from the 3rd trimester of pregnancy to 12 months postpartum. In the 3rd trimester and 2 months postpartum, women will report on their sociodemographic and obstetric characteristics. In all assessment waves, mothers will complete self-reported measures of IPV, cognitive PRF, and adult attachment. At 2 months postpartum, women’s neuro PRF will be monitored, and at 5 months postpartum, their parenting behavior will be assessed. The infant-mother attachment will be assessed at 12 months postpartum.

Discussion
Our study’s innovative focus on maternal neuro and cognitive processes and their impact on infant development will inform evidence-based early intervention and clinical practices for vulnerable infants exposed to IPV.
Prescription characteristics associated with drug overdose risk among adults prescribed benzodiazepines: a cohort study
Authors: Maust Donovan T. , Bohnert Amy S. B. , Strominger Julie , Goldstick Jason E. 
Published: May 2023

Abstract
Background
Drug overdose (OD) deaths in the U.S. continue to rise. After opioids, benzodiazepines (BZD) are the medication most commonly involved in prescription overdoses, yet OD risk factors among those prescribed BZD are not well understood. Our objective was to examine characteristics of BZD, opioid, and other psychotropic prescriptions associated with increased drug OD risk following a BZD prescription.

Methods
We completed a retrospective cohort study using a 20% sample of Medicare beneficiaries with prescription drug coverage. We identified patients with a BZD prescription (“index”) claim between 1 April 2016 and 31 December 2017. In the 6 months pre-index, those without and with BZD claims comprised incident and continuing cohorts, which were split by age (incident < 65 [n = 105,737], 65 + [n = 385,951]; continuing < 65 [n =  240,358], 65 + [n = 508,230]). Exposures of interest were: average daily dose and days prescribed of the index BZD; baseline BZD medication possession ratio (MPR) for the continuing cohort; co-prescribed opioids and psychotropics. Our primary outcome was a treated drug OD event (including accidental, intentional, undetermined, or adverse effect) within 30 days of the index BZD, examined using Cox proportional hazards.

Results
Among incident and continuing BZD cohorts, 0.78% and 0.56% experienced an OD event. Compared to 14–30 days, a < 14-day fill corresponded to higher OD risk in incident (< 65 adjusted hazard ratio [aHR] 1.16 [95% CI 1.03–1.31]; 65 + : aHR 1.21 [CI 1.13–1.30]) and continuing (< 65: aHR 1.33 [CI 1.15–1.53]; 65 + : aHR 1.43 [CI 1.30–1.57]) cohorts. Among continuing users, lower baseline exposure (i.e., MPR < 0.5) was associated with increased OD risk for those < 65 (aHR 1.20 [CI 1.06–1.36]); 65 + (aHR 1.12 [CI 1.01–1.24]). Along with opioids, concurrent antipsychotic use and antiepileptic use were associated with elevated risk of OD in all 4 cohorts (e.g., aHRs for the continuing 65 + cohort: opioid, 1.73 [CI 1.58–1.90]; antipsychotic, 1.33 [CI 1.18–1.50]; antiepileptic, 1.18 [1.08–1.30]).

Conclusions
In both the incident and continuing cohorts, patients dispensed fewer days' supply were at increased OD risk; those in the continuing cohort with more limited baseline BZD exposure were also at elevated risk. Concurrent medication exposures including opioids, antipsychotics, and antiepileptics were associated with short-term elevated OD risk.
Long-term clinical results of alumina ceramic medial pivot total knee arthroplasty: a 10-year follow-up study
Authors: Ueyama Hideki , Nakagawa Shigeru , Kishimura Yuichi , Minoda Yukihide , Nakamura Suguru , Koyanagi Junichiro , Yamamura Mitsuyoshi , Kadoya Yoshinori 
Published: May 2023

Abstract
Background
The newly-designed alumina ceramic medial pivot total knee prosthesis was introduced to reduce polyethylene wear and better fit the anatomical morphology of the Asian population. This study aimed to clarify the long-term clinical results of alumina medial pivot total knee arthroplasty over a minimum follow-up period of 10 years.

Methods
The data of 135 consecutive patients who underwent primary alumina medial pivot total knee arthroplasty were analyzed in this retrospective cohort study. Patients were examined over a minimum 10-year follow-up period. The knee range of motion, Knee Society Score (KSS) knee score, Knee Society Score function score, and radiological parameters were assessed. The survival rate was also evaluated by using reoperation and revision as endpoints.

Results
The mean follow-up period lasted 11.8 ± 1.4 years. Patients who were not followed accounted for 7.4% of the total cohort. Knee and function scores of KSS improved significantly following total knee arthroplasty (P < 0.001). In 27 individuals (28.1%), a radiolucent line was observed. Aseptic loosening occurred in three cases (3.1%). The survival rates for reoperation and revision were 94.8% and 95.8% 10 years after the operation, respectively.

Conclusions
During a minimum 10-year follow-up period, the present model of alumina medial pivot total knee arthroplasty showed good clinical outcomes and survival rates.
Multiple higher-order topological phases with even and odd pairs of zero-energy corner modes in a C3symmetry broken model
Authors: Saha Sudarshan , Nag Tanay , Mandal Saptarshi 
Published: May 2023

Abstract
Here we study emergent higher-order topological (HOTI) phases in the extended Haldane model without C
3 symmetry. For the inversion symmetric case, the QSHI and QAHI phases can embed the HOTI phases while the remaining QASHI phase does not yield any HOTI phases. Remarkably, four-fold degeneracy of zero-energy corner states can be reduced to two-fold under the application (withdrawal) of sub-lattice mass (Zeeman field) term. The sub-lattice mass and Zeeman field terms compete with each other to pin down the two mid-gap states at zero-energy. Interestingly, the bulk polarization can topologically characterize the second-order topological insulator phase with the mid-gap corner modes irrespective of their energies as long as inversion symmetry is preserved. Our study indicates that a hybrid symmetry can in principle protect the second-order topological insulator phases, however, spin-spectrum gap has to be essentially finite there.
Transnasal Humidified Rapid Insufflation Ventilatory Exchange Augments Oxygenation in Children With Juvenile Onset Recurrent Respiratory Papillomatosis During Surgery: A Prospective Randomized Crossover Controlled Trial
Authors: Lei Guiyu , Wu Lili , Xi Chunhua , Xiao Yang , Wang Guyan 
Published: May 2023

BACKGROUND:
Evidence is lacking regarding the efficacy of transnasal humidified rapid insufflation ventilatory exchange (THRIVE) in tubeless anesthesia, especially in pediatric patients. This study aimed to evaluate the use of THRIVE for juvenile onset recurrent respiratory papillomatosis (JORRP) patients.

METHODS:
Twenty-eight children aged 2 to 12 years with JORRP, abnormal airways, and ASA physical status II–III that presented for surgical treatment under general anesthesia were included in this study. Each patient received 2 interventions in random order, with a 5-minute washout period between treatments: apnea without oxygen supplementation and apnea with THRIVE intervention. The primary outcome apnea time was defined as the duration from withdrawal of intubation to reintubation and resumption of controlled ventilation. The secondary outcomes were the mean transcutaneous carbon dioxide (tcco
2) increase rate, the minimum pulse oxygen saturation (Spo
2) during apnea, and the occurrence of unexpected adverse effects.

RESULTS:
The median apnea time in the THRIVE period was significantly longer than that in the control period (8.9 [8.6–9.4] vs 3.8 [3.4–4.3] minutes; mean difference [95% confidence interval (CI)], 5.0 [4.4–5.6]; P < .001) for all patients. The rate of CO2 change in the control period was higher than that in the THRIVE period both for patients aged 2 to 5 years old (6.29 [5.19–7.4] vs 3.22 [2.92–3.76] mm Hg min−1; mean difference [95% CI], 3.09 [2.27–3.67]; P < .001) and for patients aged 6 to 12 years old (4.76 [3.7–6.2] vs 3.38 [2.64–4.0] mm Hg min−1; mean difference [95% CI], 1.63 [0.75–2.56]; P < .001). The minimum Spo
2 was significantly higher in the THRIVE period than in the control period (mean difference [95% CI], 19.7 [14.8–22.6]; P < .001).

CONCLUSIONS:
Our findings demonstrate that THRIVE safely increased the apnea time among children with JORRP undergoing surgery and decreased the rate of carbon dioxide increase. THRIVE is clinically recommended as an airway management technique for tubeless anesthesia in apneic children.
The Zero Regrets Algorithm: Optimizing over Pure Nash Equilibria via Integer Programming
Authors: Dragotto Gabriele , Scatamacchia Rosario 
Published: May 2023

Designing efficient algorithms to compute Nash equilibria poses considerable challenges in algorithmic game theory and optimization. In this work, we employ integer programming techniques to compute Nash equilibria in integer programming games, a class of simultaneous and noncooperative games in which each player solves a parameterized integer program. We introduce zero regrets, a general and efficient cutting-plane algorithm to compute, enumerate, and select Nash equilibria. Our framework leverages the concept of equilibrium inequality, an inequality valid for any Nash equilibrium, and the associated equilibrium separation oracle. We evaluate our algorithmic framework on a wide range of practical and methodological problems from the literature, providing a solid benchmark against the existing approaches. History: Accepted by Andrea Lodi, Area Editor for Design & Analysis of Algorithms – Discrete. Supplemental Material: The online supplement is available at https://doi.org/10.1287/ijoc.2022.0282 .
RUKO SEBAGAI BARRIER UNTUK MEREDUKSI KEBISINGAN AKIBAT AKFTIFITAS TRANSPORTASI DI JALAN RAYA
Authors: Purwandani Riana 
Published: May 2023

Di sepanjang Jalan Raya terdapat rumah toko ruko yang membelakangi pemukiman-pemukiman yang ada di sekitar daerah tersebut. Ruko tersebut secara tidak langsung bertindak sebagai barrier yang bermanfaat untuk melindungi pemukiman dari kebisingan akibat aktifitas transportasi di Jalan Raya Mulyosari. Dalam penelitian ini dilakukan pengukuran tingkat efisiensi ruko dalam mereduksi kebisingan berdasarkan variabel ketinggian ruko dan jarak antara ruko dengan sumber bising. Tingkat kebisingan diukur dengan menggunakan SLM (Sound Level Meter). Penelitian ini mengukur kebisingan di depan dan dan di belakang ruko. Ketinggian ruko yang diteliti adalah ruko yang memilki ketinggian 5-9 meter, 9-13 meter dan 13-16 meter. Sedangkan variabel jarak antara ruko dengan sumber bising yang diteliti adalah pada ruko yang memiliki jarak dengan sumber bising 0–4 meter, 4-8 meter dan 8-12 meter.