bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction land reduction projects as a pathway to sustainability: an empirical analysis of risks factors in China
Authors: Mu Bingxu , Zhu Yuming , Ahmad Naveed , Zhou Jiahe , He Lei , Lin Hongli 
Published: May 2023

Abstract
Construction land reduction (CLR) is an effective instrument to improve intensive land use, restrict the expansion of construction land, safeguard the requisition-compensation balance of construction land in China, and realize sustainable development. But multiple risks arise from the process of construction land reduction. In that case, identifying and analyzing the key risks of CLR is the prerequisite for formulating practical policy guidelines. This study is conducted to identify the risk factors of CLR and analyze these risks based on expert opinion. Following an in-depth review of the literature, we create a contextual relationship-based model employing an integrated technique of Interpretive Structural Modeling (ISM) and Cross-Impact Matrix Multiplication Applied to Classification (MICMAC) analysis. Based on the ISM and MICMAC analysis, five key risks were identified, and the prevention strategies and policy recommendations for CLR project risks are put forward.
CDCA8 promotes bladder cancer survival by stabilizing HIF1α expression under hypoxic
Authors: Guo Ju , Zhou Qiang , Huang Wei , Xiong Jing , Guo Biao , Wang Xinghuan 
Published: May 2023

Abstract
Hypoxia is an essential feature of solid tumors. The regulatory network behind tumor cells in response to hypoxia environment is not completely comprehend. We ascertained the biochemical role of cell cycle division-related gene 8 (CDCA8) in bladder cancer (Bca) survival under hypoxia environment. In current study we revealed the expression of CDCA8 was considerably upraised in BCa. High expression level of CDCA8 was positive related with advanced Bca stage, advanced Bca stage grade and poor survival. Increased CDCA8 expression was decisive for Bca cells to survive in a hypoxic condition. CDCA8 enhanced the stabilization of HIF1α by competitively binding to AKT with PTEN and alleviating PTEN suppression to activate AKT phosphorylation, while HIF1α transcriptionally promote CDCA8 transcription, thus forming a positive feedback loop in Bca adaptation to oxygen-deficient environment. Collectively, CDCA8 promotes Bca survival under hypoxic environment by activating AKT/GSK3β signaling pathway and heightening the stabilization of HIF1α. CDCA8 is critical for Bca to adapt to oxygen deprivation and may provide a novel thinking for Bca treatment.
The Perceived Burden Among Caregivers of Deinstitutionalized Patients with Severe Mental Illness in Malaysia’s Capital City: An Interviewer Administered Survey
Authors: Ismail Halim , Ahmad Hanis , Rizal Haffiz , Hannan Wan , Azmi Fikri , Reffin Naiemy , Chan David , Baharudin Hafiz , Aris Azila , Shen Huam Zhe , Hakimi Hibatul 
Published: May 2023

Abstract

Background: Patients with severe mental illness often linked to a severe decline in social abilities due to disorganized behaviours depend entirely on primary caregivers. Nonetheless, without formal payment and visible reward, this force inclined into a caregiving position interfered with caregivers’ daily functions and psychological condition. Thus, our study aims to determine the prevalence of caregiver’s burden, psychological implications and associated predictors among the caregivers of severely mentally ill patients in Malaysia's capital city, Kuala Lumpur.
Methods: An interviewer-administered, cross-sectional study was conducted within six months-period involving 149 primary caregivers from outpatient psychiatric units of tertiary hospitals in Kuala Lumpur, who were selected using the single-level cluster sampling technique. The caregiver burden and psychological implication were evaluated using the validated local version of the Zarit-load-Interview Score and the DASS-21. At the same time, the association with its predictors were analyzed with a logistic model.
Results: The mean ZBI score was 31.69 ± 18.52, with 34.6% of participants experiencing caregiver burden; the majority (16.8%) suffered from a moderate burden. Anxiety is the participants' most prevalent psychological implication, accounting for 29.3%, depression at 22.1% and stress at 11.7%. Multivariate analysis indicated that the odds of experiencing burden were 29 times higher among those who concurrently have symptoms of depression, four times higher among male caregivers, and three times higher among those caring for schizophrenia patients. Being a spouse, nonetheless, reduced the risk of experiencing a burden by 84%.
Conclusion: Results indicated a significant prevalence of burden among the caregiver of severely mental-ill patients in Kuala Lumpur, Malaysia. Among those who are male, concurrently have symptoms of depression, and provide care for schizophrenia patients are substantially associated with caregiving burden. Rigorous screening activity and support-need assessment focusing on high-risk groups are crucial. A national holistic support module and additional respite centre must be formed immediately. Without appropriate intervention, they will continue at risk of exposure to the complication of caregiver burden.
GSDMD promotes neutrophil extracellular traps via mtDNA-cGAS-STING pathway during lung ischemia/reperfusion.
Authors: Lin Fei , zhao chen , Liang Fangte , Ye Mengling , Wu Siyi , Qin Yi , Zhao Lu , Zhang Lu Lu , He Jing , Cen Liming 
Published: May 2023

Abstract
Lung ischemia/reperfusion injury (LIRI) is a complex pathophysiological process, with the histopathological hallmark of neutrophils migratinginto the lungs. Neutrophil extracellular traps (NETs) have been suggested to exert a critical role in the pathogenesis of inflammation and infection in human and animals, while the exact functions and underlying mechanisms of NETs in LIRI remain insufficiently elucidated. In this study, we investigated the role of pore-forming protein gasdermin D (GSDMD) on NETs release in LIRI induced by lung ischemia/reperfusion (I/R). We found that disulfiram, a GSDMD inhibitor, dramatically reduced NETs release and pathological injury in lung I/R in vivoand in vitro. Additionally, GSDMD caused mitochondrial DNA (mtDNA) leaking into the neutrophil cytosol, and then the cytoplasmic mtDNA activated the cGAS-STING signaling pathway and stimulated NETs formation in lung I/R. Furtherly, inhibition of cGAS/STING pathway could inhibit cytosol mtDNA mediated NETs formation.
First report on Molecular Characterization of Oesturs ovis in sheep from India
Authors: Nagarajan Govindasamy , Kanagarajadurai K , Pachaiyappan K , Pandian S Jegaveera , Thirumurugan P , Rajendiran A S 
Published: May 2023

Abstract
Purpose Upon post mortem examination of sheep (from SRRC, Mannavanur, South India) having the history of natural death, the nasal sinuses of the animals contained dipteran larvae. The objective of the present study is to characterize the dipteran larvae species infesting the sheep being maintained at SRRC, Mannavanur, by means of COI gene based PCR.
Methods During the last week of May 2021, an Avikalin male sheep (20 months old) died suddenly. Upon Post mortem examination, the skull of the dead sheep was having of larvae in the nasal sinuses. The larvae were washed in PBS (pH 7.2) and preserved in 70 % alcohol. The larvae taken out of 70 % alcohol were thoroughly ground to make a fine powder using liquid Nitrogen in a sterile mortar and pestle. The maggot in powder form was then used for the isolation of total genomic DNA isolation. Using the isolated genomic DNA from the larvae as a template, Cytochrome c oxidase subunit I (COI) gene based PCR was employed using the primers designed based on the COI gene of reference isolate of Oestrus ovis available in the GenBank. Full length COI gene (1534 bp) gene of Oestrus ovis in sheep from South India was targeted in the PCR experiment. The pTZ57R/T vector was used for the cloning of the PCR amplified fragment and the confirmed recombinant plasmid was subjected to sequencing experiments. The resultant nucleotide sequences of COI gene of the O.ovis in sheep from South India was analysed with that of 29 dipteran species from different different geographical areas of the world (available in GenBank) by using standard bioinformatics tools.
Results In addition to morphological examination, based on COI gene based PCR, eventual sequencing experiments and BLAST analysis, it was confirmed that the larvae in the nasal sinuses of sheep from South India were Oestrus ovis. The South Indian isolate of Oestrus ovis is sharing 100% sequence identity both at nucleotide and amino acid levels with that of O.ovis from Spain. The North Indian isolate of O.ovis (from Jammu) exhibited 92 & and 99 % identity at respective nucleotide and amino acid levels with South Indian isolate. With other members of the subfamily Oestrinae, the share of per cent nucleotide and amino acid identities of South Indian O.ovis ranged from 85-86 % and 95-96 %, respectively. O.ovis from South India was grouped with the other members of Oestrinae from different geographical areas of the globe in the analysis of phylogenetic tree based on COI amino acid sequences.
ConclusionBased on the research findings, it is concluded that Oestrus ovis is the dipteran species infesting the sheep at Mannavanur, Tamil Nadu, India. To our knowledge, this is the first report on full length nucleotide sequences of COI gene of O.ovis in sheep from Indian subcontinent. An investigation on the report of human ocular infections and the accidental hosts for O ovis in the area of the present study has to be explored.
The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice
Authors: Sugano Shigeo S , Nakamura Akiyoshi , Furubayashi Maiko , Mitsuda Nobutaka , Yano Tsubasa , Terakawa Teruhiko , Ito Seiichiro 
Published: May 2023

Abstract
CRISPR/Cas9-based genome editing represents an unprecedented potential for plant breeding. Unlike animal cells, plant cells contain a rigid cell wall, genome editing tool delivery into plant cells is thus challenging. In particular, the delivery of the Cas9-gRNA ribonucleoprotein (RNP) into plant cells is desired since the transgene insertion into the genome should be avoided for industrial applications in plants. In this study, we present a novel RNP delivery approach in rice. We applied the sonication-assisted whisker method, conventionally developed for DNA delivery in plants, for RNP delivery in rice. Combined with marker gene delivery, we successfully isolated LCYβgenome-edited lines generated by RNPs. The calli and regenerated shoot of the LCYβmutant showed abnormal carotenoid accumulation. In addition, we also detected, although at a low frequency, genome editing events in rice calli cells by RNP delivery using the sonication-assisted whisker method without any additiona. Therefore, the sonication-assisted whisker method could be an attractive way to create RNP-based genome-edited lines in plants.
Chemical Composition and antimicrobial activity of essential oils of two wild olive subspecies Olea europaea subsp. europaea var. Sylvestris and the endemic Olive Olea europaea subsp.lapperinie from Algeria
Authors: Bouchoucha Sara , BOUKHEBTI Habiba , Oulmi Abdemalek , Mouhamadi Yacine , Chaker Adel Nadjib 
Published: May 2023

Abstract

Leaves of the Olea europaea L. species are frequently utilized in both folk and modern medicine.In nowadays are strongly valuable for their beneficial effects to treat a multitude of bacterial and inflammation-type diseases, two wild olive subspecies fixed in this research: the widespread Mediterranean Olea europaea subsp. europaea var. sylvestris and the endemic Olea europaea subsp. laperrinei from Saharan region, Despite its ecological value, the chemical composition of subsp. laperrinei oil remains unknown, the samples were harvested from the different geographical area. Gas Chromatography-Mass Spectrometry(GC-MS) and Gas Chromatography-flame-ionization detection (GC-FID) analysis of subsp europaea var. sylvestris allowed the identification of 29 compounds oil with Nonanal (11.82%), Theaspiranea A (9.81%), 3-hexen-1-ol,benzoate(9.31%), β-damascenone (6.87%), α-pinene (6.32%), Farnesol (6.10%) as a major constituents, while the subspecies of the Saharan region were resultedthe identification of 31 compounds where α-pinene (16%), β-Ocimene (12.82%), dl-Limonene(8.20%), Nonanal (8.18%), was the main components .The Monoterpene hydrocarbons represented the most abundant class in the two essential oils samples. The results of the disc diffusion method showed that the two volatile oils have efficient antibacterial activity but, subsp. laperrinei essential oil has a higher range of inhibition, in which P. aeruginosa and B. subtilis showed extreme sensitivity, while the K. pneumoniae bacterium shows great resistance to the two essential oils. These results demonstrated the medicinal value of olive leaves, as well as the impact of climate on the volatiles produced.
Defining nutrient ecoregions for reference nitrogen and phosphorus concentrations in rivers from the major South American biomes
Authors: Tromboni Flavia , Dodds Walter , Cunha Davi , Monteiro José , Avocat Helene , Caldas Marcellus , Gücker Björn 
Published: May 2023

Abstract

Knowing reference (i.e., baseline) riverine nutrient concentrations is essential to understand fundamental processes of biogeochemical transport from continents to the ocean, describing ecological conditions, and informing managers of best attainable conditions for nutrient abatement and controlling anthropogenic eutrophication. We used data from 434 Brazilian watersheds representative of six South American biomes to estimate expected nutrient levels in the absence of anthropogenic influence. We used a novel watershed-based approach to describe spatial patterns throughout Brazil and for the entire Amazon basin. We found reference total nitrogen concentrations were most closely related to biome, whereas total phosphorus levels were to soil properties in addition to climatic factors influencing biomes. We found that many areas have intrinsically high background phosphorus and relatively low nitrogen, suggesting nitrogen-limitation of freshwaters could be widespread in South America, and nitrogen-fixing cyanobacterial blooms could have a competitive advantage. These results represent the first broad-scale analyses of spatial distribution of baseline nutrient levels for tropical and subtropical waters.
Trans-spinal direct current stimulation affects the corticospinal system but does not affect motor skills
Authors: Pomelova Ekaterina , Popyvanova Alena , Bredikhin Dimitri , Koriakina Maria , Ilyukina Natalya , Shestakova Anna N. , Blagovechtchenski Evgeny 
Published: May 2023

Abstract

The purpose of the study was to investigate the effect of anodal trans-spinal direct current stimulation (tsDCS) applied at the level of cervical spinal cord enlargement (C7–Th1 segments) on corticospinal system excitability and motor skills. The study involved 54 healthy adults aged 21.19 ± 3.2 years. Our results showed that the application of an anodal tsDCS (11-minute, 1.5 mA) affected the first dorsal interosseous muscle of the index finger when measured by motor evoked potentials (MEPs) elicited by transcranial magnetic stimulation of the primary motor cortex. The effect of tsDCS manifested as a decrease in MEP amplitude immediately following stimulation and an increase in amplitude 15 minutes after it. The statistical analysis demonstrated that the dynamics of MEP amplitudes differed between groups receiving anodal tsDCS and sham stimulation, although anodal tsDCS did not affect motor skills production. The ability of an individual after receiving anodal tsDCS to coordinate their fingers and manipulate objects effectively in the nine-hole peg test and pressing a key in response to visual stimulus in the serial reaction time task did not differ from the sham stimulation. We also found that the motor learning factor during stimulation did not affect the change in MEP amplitude.
Leveraging Bidirectionl LSTM with CRFs for Pashto tagging
Authors: Zaman Farooq , Maqbool Onaiza , Kanwal Jaweria 
Published: May 2023

Abstract
Part-of-speech tagging plays a vital role in text processing and natural language understanding. Very few attempts have been made in the past for tagging Pashto Part-of-Speech. In this work, we present LSTM based approach for Pashto part-of-speech tagging with special focus on ambiguity resolution. Initially we created a corpus of Pashto sentences having words with multiple meanings and their tags. We introduce a powerful sentences representation and new architecture for Pashto text processing. The accuracy of the proposed approach is compared with state-of-the-art Hidden Markov Model. Our Model shows 87.60\% accuracy for all words excluding punctuations and 95.45\% for ambiguous words, on the other hand Hidden Markov Model shows 78.37\% and 44.72\% accuracy respectively. Results show that our approach outperform Hidden Markov Model in Part-of-Speech tagging for Pashto text.
Arbuscular mycorrhizae selectively suppress soil ammonia oxidizers – but not via constitutive production of biological nitrification inhibitors
Authors: Sun Daquan , Kotianová Michala , Rozmoš Martin , Hršelová Hana , Bukovská Petra , Jansa Jan 
Published: May 2023

Abstract
Aims: Arbuscular mycorrhizal (AM) fungi forming root symbioses can improve acquisition of nutrients including nitrogen (N) from soils, which could lead to host plant growth improvements. We previously showed that application of AM fungal necromass to the rhizosphere could stimulate plant growth by as yet unknown mechanism. Here we tested how the effects on plant N could be explained by interactions between AM fungi, living or dead, and one important group of soil prokaryotes in soil N cycling, the ammonia oxidizers (AO) carrying out the first step of nitrification. We were interested in whether the necromass contained any significant biological nitrification inhibitory (BNI) activity that could explain previously suggested antagonism between the AM fungi and the AO.
Methods: To this end we set up a compartmented pot experiment with Andropogon gerardii as a host plant, which included rhizosphere (amended with living or dead AM fungal biomass) and a root-free zone. The latter contained a mesh bag with 15N-labelled plant litter.
Results: Inoculation with living AM fungi generally enhanced plant biomass and nutrient acquisition, including the 15N transfer from mesh bags to the plants, while significantly suppressing AO bacterial but not archaeal populations in the mesh bags. In contrast, the amendment with AM fungal necromass had minimal effect on plant biomass and nutrient acquisition, but actually stimulated both AO bacteria and archaea in the rhizosphere of non-mycorrhizal plants.
Conclusions: Thus we reject the possibility that AM fungi suppress the AO via an elusive BNI production but rather the substrate (ammonium) competition.
First Observation of Fluid-like Eddy-dominant Bursty Bulk Flow Turbulence in the Earth’s Tail Plasma Sheet
Authors: Zhang L. Q. , Wang Chi. , Baumjohann W. , Wang R.S , Wang J. Y. , Burch James L. , Khotyaintsev Yu. V. 
Published: May 2023

Abstract
Turbulence is a ubiquitous phenomenon in neutral and conductive fluids. According to classical theory, turbulence is a rotating flow containing vortices of different scales. Eddies play a fundamental role in the nonlinear cascade of kinetic energy at different scales in turbulent flow. In conductive fluids, the Alfvénic/kinetic Alfvénic wave (AW/KAW) is the new "cell" of magnetohydrodynamic (MHD) turbulence (frozen-in condition). Wave energy, which has equal kinetic and magnetic energy, is redistributed among multiple-scale Fourier modes and transferred from the large MHD scale to the small kinetic scale through the collision of counter-propagating Alfvénic wave packages propagating along the magnetic field line. Fluid-like eddy-dominant plasma flow turbulence has never been found in space since the launch of the first satellite in 1957. In this paper, we report the first observation of eddy-dominant turbulence within magnetic reconnection-generated fast flow in the Earth’s tail plasma sheet by the Magnetospheric Multiscale Spacecraft (MMS). Our findings shed new light on the nonlinear kinetic and magnetic energy cascade in MHD turbulence.
Association between BNT162b2 vaccination and health-related quality of life up to 18 months post-SARS-CoV-2 infection in Israel: A cross sectional survey
Authors: Edelstein Michael , Kuodi Paul , Gorelik Yanay , Zayyad Hiba , Wertheim Ofir , Wiegler Karine Beiruti , Jabal Kamal , Dror Amiel , Elsinga Jelte , Nazzal Saleh , Glikman Daniel 
Published: May 2023

Abstract
We determined whether COVID-19 vaccination was associated with Quality of Life (QoL) changes among individuals previously infected with SARS-CoV-2 in Israel. Using a validated questionnaire, we collected information about socio-demographics, SARS-CoV-2 infection, COVID-19 vaccination and QoL (using the EQ-5D-5L tool) 3–18 months post-infection among adults tested for SARS-CoV-2 by polymerase chain reaction in Northern Israel between March 2020-June 2022. We compared post-COVID QoL between those vaccinated against COVID-19 at the time of infection and those not, using an adjusted linear regression model, stratified by time elapsed since infection. Of 951 participants, mean EQ-5D Utility Index (EQ-5D UI) was 0·82 (SD = 0·26) and 0·83 (SD = 0·25) among the 227 double and 250 triple vaccinated respectively, compared to 0·76 (SD = 0·33) among those who received 0 dose (n = 243). In the adjusted model, previously infected individuals vaccinated with two or more doses reported a 0·05 increase in QoL score post- infection (CI = 0·01–0·10, p = 0·02) compared with those unvaccinated when infected. No association between vaccination and QoL was detected beyond 12 months post-infection. Vaccination with two or more doses of COVID19 vaccine, or at least the BNT162b2 vaccine, may partly mitigate QoL losses associated with post-acute COVID-19 symptoms, at least in the first 12 months post-infection.
Estrogen receptor α confers Nab-paclitaxel resistance in breast cancer by promoting miR199a-5p maturation to inhibit Caveolin 1 translation
Authors: Zhang Jianping , Wang Zuo , Zhu Liyuan , Wang Chaoqun , Zhong Yiming , Ying Pingting , Wang Hanying , Li Qinglin , Feng Lifeng , Wang Xian , Jin Hongchuan 
Published: May 2023

Abstract
Background Estrogen receptor positive (ER+) breast cancer patients are poorly responsive to Nab-paclitaxel compared to ER negative (ER-) breast cancer patients. Herein, we conducted an investigation regarding the mechanism for ERα confers Nab-paclitaxel resistance in breast cancer.Methods Retrospectively reviewed 116 cases of breast cancer treated with nab-paclitaxel between Jan 2008 and May 2022 in Sir Run Run Shaw Hospital. StataSE 16 software was used to analyze the basic conditions and therapeutic effects. Protein-RNA interactions were validated through RNA immunoprecipitation and RNA pull-down assays. In vitro and in vivo experiments were carried out to testify the effect of ERα on Nab-paclitaxel resistance.Results We show that ERα limits the efficacy of nab-paclitaxel in breast cancer while genetic or pharmacological inhibition of ERα has a synergistic effect with Nab-paclitaxel. Meanwhile, CAV1 expression is negatively correlated to ERα and relevant to the better clinical benefits of Nab-paclitaxel treatment. Importantly, ERα stimulates miR199a-5p maturation to antagonize m6A modification of CAV1 mRNA, thus inhibiting its translation.Conclusions Our results define a novel role of ERα miR199a-5p/CAV1 axis responsible for nab-paclitaxel resistance and propose combining ER antagonist with nab-paclitaxel as a perspective strategy for ER + breast cancer patients.