bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Arabidopsis Target of Rapamycin kinase regulates ammonium assimilation and glutamine metabolism
Authors: Ingargiola Camille , Jéhanno Isabelle , Forzani Céline , Marmagne Anne , Broutin Justine , Clément Gilles , Leprince Anne-Sophie , Meyer Christian 
Published: April 2023

AbstractIn eukaryotes, a target of rapamycin (TOR) is a well-conserved kinase that controls cell metabolism and growth in response to nutrients and environmental factors. Nitrogen (N) is an essential element for plants, and TOR functions as a crucial N and amino acid sensor in animals and yeast. However, knowledge of the connections between TOR and the overall N metabolism and assimilation in plants is still limited. In this study, we investigated the regulation of TOR in Arabidopsis (Arabidopsis thaliana) by the N source as well as the impact of TOR deficiency on N metabolism. Inhibition of TOR globally decreased ammonium uptake while triggering a massive accumulation of amino acids, such as Gln, but also of polyamines. Consistently, TOR complex mutants were hypersensitive to Gln. We also showed that the glutamine synthetase inhibitor glufosinate abolishes Gln accumulation resulting from TOR inhibition and improves the growth of TOR complex mutants. These results suggest that a high level of Gln contributes to the reduction in plant growth resulting from TOR inhibition. Glutamine synthetase activity was reduced by TOR inhibition while the enzyme amount increased. In conclusion, our findings show that the TOR pathway is intimately connected to N metabolism and that a decrease in TOR activity results in glutamine synthetase-dependent Gln and amino acid accumulation.
A non-cell-autonomous circadian rhythm of bioluminescence reporter activities in individual duckweed cells
Authors: Watanabe Emiri , Muranaka Tomoaki , Nakamura Shunji , Isoda Minako , Horikawa Yu , Aiso Tsuyoshi , Ito Shogo , Oyama Tokitaka 
Published: April 2023

Abstract
The circadian clock is responsible for the temporal regulation of various physiological processes in plants. Individual cells contain a circadian oscillator consisting of a clock gene circuit that coordinates physiological rhythms within the plant body in an orderly manner. The coordination of time information has been studied from the perspective of cell–cell local coupling and long-distance communication between tissues based on the view that the behavior of circadian oscillators represents physiological rhythms. Here, we report the cellular circadian rhythm of bioluminescence reporters that are not governed by the clock gene circuit in expressing cells. We detected cellular bioluminescence rhythms with different free-running periods in the same cells using a dual-color bioluminescence monitoring system in duckweed (Lemna minor) transfected with Arabidopsis CIRCADIAN CLOCK ASSOCIATED 1::luciferace+ (AtCCA1::LUC+) and Cauliflower mosaic virus 35S::modified click-beetle red-color luciferase (CaMV35S::PtRLUC) reporters. Co-transfection experiments with the two reporters and a clock gene-overexpressing effector revealed that the AtCCA1::LUC+ rhythm, but not the CaMV35S::PtRLUC rhythm, was altered in cells with a dysfunctional clock gene circuit. This indicated that the AtCCA1::LUC+ rhythm is a direct output of the cellular circadian oscillator, whereas the CaMV35S::PtRLUC rhythm is not. After plasmolysis, the CaMV35S::PtRLUC rhythm disappeared, whereas the AtCCA1::LUC+ rhythm persisted. This suggests that the CaMV35S::PtRLUC bioluminescence has a symplast/apoplast-mediated circadian rhythm generated at the organismal level. The CaMV35S::PtRLUC-type bioluminescence rhythm was also observed when other bioluminescence reporters were expressed. These results reveal that the plant circadian system consists of both cell-autonomous and noncell-autonomous rhythms that are unaffected by cellular oscillators.
Intestinal uric acid excretion contributes to serum uric acid decrease during acute gout attack
Authors: Zhao Tianyi , Cao Ling , Lin Cong , Xu Rui , Du Xingchen , Zhou Mengmeng , Yang Xue , Wan Weiguo , Zou Hejian , Zhu Xiaoxia 
Published: April 2023

Abstract

Objective
Spontaneous serum uric acid (SUA) decrease has been found in many patients during acute gout attacks, but its mechanism remains unclear.

Methods
The spontaneous regulation of SUA during a gout attack and its possible causes were evaluated in patients with gout. The mechanism of the spontaneous SUA decrease was further studied in Caco2 cells and a monosodium urate (MSU)-induced gout model of wild-type mice and ABCG2−/− mice. The urate transport function of intestinal epithelial cells was detected by transwell culture of Caco2 cells. Expression of ATP-binding cassette super-family G member 2 (ABCG2), IL-1β and phosphoinositide 3-kinase (PI3K)/Akt was analysed using real-time PCR, western blotting, or immunofluorescence assays.

Results
SUA decreased during acute gout attacks in both the gout patients and MSU-induced gouty mice. Increased serum CRP and IL-1β levels were correlated with the SUA decrease. Intestinal uric acid excretion and expression of ABCG2 were upregulated in the mice during acute gout attacks. In the ABCG2−/− mice, intestinal uric acid excretion significantly decreased during gout attacks. In an in vitro study of a transwell culture, ABCG2 and its upstream PI3K/Akt pathway were significantly upregulated in intestinal epithelial cells. However, ABCG2 expression and its associated intestinal uric acid transport were inhibited when PI3K/Akt was blocked by a PI3K inhibitor, LY294002.

Conclusions
Increased intestinal urate excretion resulted in spontaneous SUA downregulation during acute gout attacks. Inflammation-induced PI3K/Akt activation and ABCG2 expression in epithelial cells might contribute to the upregulation of intestinal uric acid excretion.
Evaluation of research proposals by peer review panels: broader panels for broader assessments?
Authors: Abma-Schouten Rebecca , Gijbels Joey , Reijmerink Wendy , Meijer Ingeborg 
Published: April 2023

Abstract
Panel peer review is widely used to decide which research proposals receive funding. Through this exploratory observational study at two large biomedical and health research funders in the Netherlands, we gain insight into how scientific quality and societal relevance are discussed in panel meetings. We explore, in ten review panel meetings of biomedical and health funding programmes, how panel composition and formal assessment criteria affect the arguments used. We observe that more scientific arguments are used than arguments related to societal relevance and expected impact. Also, more diverse panels result in a wider range of arguments, largely for the benefit of arguments related to societal relevance and impact. We discuss how funders can contribute to the quality of peer review by creating a shared conceptual framework that better defines research quality and societal relevance. We also contribute to a further understanding of the role of diverse peer review panels.
Application of the Ohio Nurse Competency Model in a BSN Leadership/Management Course
Authors: Keib Carrie , Barnett Carolyn , Huang Chu-Yu 
Published: April 2023

Abstract
Strategically preparing students to meet practice expectations is a priority for nursing educators. The Ohio Nurse Competency Model (ONCM) was applied as the organizing framework for a senior baccalaureate leadership/management course. The purpose of this pretest/posttest descriptive study was to evaluate changes in students’ confidence in understanding, applying, and valuing the ONCM. Paired t-tests found statistically significant increases in confidence levels for all ONCM competencies among 56 students with greatest increases in systems-based practice and informatics and technology. Nurse educators are encouraged to intentionally structure and organize course content and curricula according to state and national competency models/guidelines.
What can we learn about selective attention processes in individuals with chronic pain using reaction time tasks? A systematic review and meta-analysis
Authors: Abudoush Ahmad N. , Noureen Amna , Panagioti Maria , Poliakoff Ellen , Van Ryckeghem Dimitri M.L. , Hodkinson Alexander , Husain Nusrat 
Published: April 2023

Abstract
Information-processing biases such as attentional, interpretation, and memory biases are believed to play a role in exacerbating and maintaining chronic pain (CP). Evidence suggests that individuals with CP show attentional bias toward pain-related information. However, the selective attentional processes that underpin this bias are not always well outlined in the literature. To improve current understanding, a systematic review was performed using a descriptive synthesis of reaction time-based studies. A random-effects meta-analysis was added to explore whether the results of previous meta-analyses would be confirmed using studies with a larger sample size. For this review, 2008 studies were screened from 4 databases, of which 34 (participant n = 3154) were included in the review and a subset of 15 (participant n = 1339) were included in the meta-analysis. Review results were summarised by producing a descriptive synthesis for all studies. Meta-analysis results indicated a mild significant attentional bias toward sensory pain-related information (k = 15, g = 0.28, 95% CI [0.16, 0.39], I2 = 43.2%, P = 0.038), and preliminary evidence of significant moderate bias towards affective pain-related information (k = 3, g = 0.48, 95% CI [0.23, 0.72], I2 = 7.1%, P = 0.341) for CP groups compared with control groups. We explored the main tasks, stimuli, and CP subtypes used to address attentional biases and related processes. However, variation across studies did not allow for a decisive conclusion about the role of stimulus, task type, or related attentional processes. In addition, a table of CP attention-related models was produced and tested for reliability. Finally, other results and recommendations are discussed.
Best Practices for Implementation of an Employee Health COVID-19 Vaccine Clinic—A Model for Future Pandemic Preparedness
Authors: Green-McKenzie Judith , Field-Flowers Catherine , Peairs Kimberly , Barnes Kenneth , Shofer Frances , Kuter Barbara J. 
Published: April 2023

Objectives
The aims of the study are to determine best practices from two large-scale, academic medical centers’ employee coronavirus 2019 (COVID-19) vaccination clinics and to apply them to create scalable modules for rapid administration of 10,000 vaccinations.

Methods
The weekly number of COVID-19 vaccine doses administered was captured. Processes were compared to determine best practices, which informed the scalable financial model.

Results
Within the first 3 months, more than 60,000 COVID-19 vaccine doses were administered, and 70% of employees were fully vaccinated in 4 months with more than 95% by the vaccine mandate deadline. The estimated cost of delivering one dose was $29.95 ($299,505/10,000) compared with $35–$39 per dose when delivered by an on-site retail pharmacy.

Conclusions
Successful, safe, and rapid delivery of more than 60,000 COVID-19 vaccine doses in 3 months is practical and scalable. Learnings go beyond COVID-19 and can be applied to future outbreaks/pandemics.
Predicting long-term risk of reoperations following abdominal and pelvic surgery: a nationwide retrospective cohort study
Authors: Toneman Masja K. , Krielen Pepijn , Jaber Ahmed , Groenveld Tjitske D. , Stommel Martijn W.J. , Griffiths Ewen A. , Parker Mike C. , Bouvy Nicole D. , van Goor Harry , Ten Broek Richard P.G. 
Published: April 2023

Background:
The risk of reoperations after abdominal and pelvic surgery is multifactorial and difficult to predict. The risk of reoperation is frequently underestimated by surgeons as most reoperations are not related to the initial procedure and diagnosis. During reoperation, adhesiolysis is often required, and patients have an increased risk of complications. Therefore, the aim of this study was to provide an evidence-based prediction model based on the risk of reoperation.

Materials and methods:
A nationwide cohort study was conducted including all patients undergoing an initial abdominal or pelvic operation between 1 June 2009 and 30 June 2011 in Scotland. Nomograms based on multivariable prediction models were constructed for the 2-year and 5-year overall risk of reoperation and risk of reoperation in the same surgical area. Internal cross-validation was applied to evaluate reliability.

Results:
Of the 72 270 patients with an initial abdominal or pelvic surgery, 10 467 (14.5%) underwent reoperation within 5 years postoperatively. Mesh placement, colorectal surgery, diagnosis of inflammatory bowel disease, previous radiotherapy, younger age, open surgical approach, malignancy, and female sex increased the risk of reoperation in all the prediction models. Intra-abdominal infection was also a risk factor for the risk of reoperation overall. The accuracy of the prediction model of risk of reoperation overall and risk for the same area was good for both parameters (c-statistic=0.72 and 0.72).

Conclusions:
Risk factors for abdominal reoperation were identified and prediction models displayed as nomograms were constructed to predict the risk of reoperation in the individual patient. The prediction models were robust in internal cross-validation.
Association Between Magnetic Resonance Imaging Findings and Time to Return to Sport After Muscle Injuries in High-Level Youth Athletes
Authors: Martínez-Silván Daniel , Wik Eirik Halvorsen , Arnáiz Javier , Farooq Abdulaziz , Mónaco Mauricio 
Published: April 2023

Objective:
To describe the relationship between magnetic resonance imaging (MRI) findings and time to return to sport (RTS) from muscle injuries in youth athletes.

Design:
Prospective collection of injury surveillance data over 6 seasons (2014-2015 to 2019-2020) and reanalysis of MRIs by a radiologist blinded to RTS time after the data collection period.

Setting:
National sports academy and sports medicine hospital.

Participants:
Male youth (11-19 years) athletes participating in the football (soccer) or athletics (track and field) programs with a muscle injury.

Independent Variables:
Magnetic resonance imaging findings (eg, grade and location) in time-loss muscle injuries.

Main Outcome Variables:
Injury incidence, distributions, and RTS time.

Results:
In total, 353 time-loss muscle injuries were recorded for 1089 athlete-seasons, of which 85 satisfied our inclusion criteria (MRI within 14 days). Return to sport time was significantly longer for reinjuries compared with index injuries. Associations between MRI findings and RTS time were specific to the muscle group, with significant differences in RTS time observed between categories of structure, location, and grade. The largest number of injuries was to the hamstrings (n = 46), where injuries involving the tendon were more severe than those involving the myotendinous junction and muscle, proximal injuries were more severe than mid-portion and distal, and longer RTS time was observed for higher injury grade (P < 0.05).

Conclusions:
Muscle injuries in youth are diverse and cannot be treated as a single injury type. Specific injury durations can be expected depending on injury characteristics such as reinjury, location, structure, and grade.
Physical Contact and Suspected Injury Rates in Female versus Male Youth Ice Hockey: A Video-Analysis Study
Authors: Williamson Rylen A. , Cairo Alexis L. , Heming Emily E. , Kolstad Ash T. , Hagel Brent E. , Emery Carolyn A. 
Published: April 2023

Objective:Canada's national winter sport of ice hockey has high youth participation; however, research surrounding female ice hockey is limited and the injury burden remains high. This study compared rates of head contact (HC), body checking (BC; high-intensity player-to-player contact), and suspected concussion between female and male youth ice hockey.Design:Cross-sectional.Setting:Game video-recordings captured in Calgary, Canada.Participants:Ten female (BC prohibited) and 10 male (BC permitted) U15 elite AA (13-14-year-old) game video-recordings collected in the 2021 to 22 seasons and 2020 to 21, respectively.Assessment of Risk Factors:An analysis of player-to-player physical contact and injury mechanisms using video-analysis.Main Outcome Measures:Videos were analyzed in Dartfish video-analysis software and all physical contacts were coded based on validated criteria, including HCs (direct [HC1], indirect [HC2]), BC (levels 4-5 on a 5-point intensity scale), and video-identified suspected concussions. Univariate Poisson regression clustering by team-game offset by game-length (minutes) were used to estimate incidence rates and incidence rate ratios (IRR, 95% confidence intervals).Results:The female game had a 13% lower rate of total physical contacts (IRR = 0.87, 0.79-0.96) and 70% lower rate of BC (IRR = 0.30, 0.23-0.39). There were however no differences in the rates of direct HC (IRR = 1.04, 0.77-1.42) or suspected concussion (IRR = 0.42, 0.12-1.42) between the cohorts. Although prohibited in the female game, only 5.4% of HC1s and 18.6% of BC resulted in a penalty.Conclusions:The rates of HC1s and suspected concussions were similar across youth ice hockey. BC rates were lower in the female game, yet still prevalent despite being prohibited.
Recent evidence of advanced preservation rhinoplasty
Authors: Ferreira Miguel G. , Santos Mariline 
Published: April 2023

Purpose of review
The philosophy of preservation rhinoplasty originated 100 years ago. Many variations have appeared in the last 10 years, with different opinions emerging in the literature and scientific meetings.

Recent findings
New technological devices (piezo and diamond burs), new anatomical concepts (W and E-points, lateral and dorsal keystone area), and many new surgical strategies have led to a recent revolution in rhinoplasty.

Summary
Preservation rhinoplasty is a surgical strategy/philosophy that has gained popularity among rhinoplasty surgeons. Dorsal preservation rhinoplasty is the ‘queen’ of preservation rhinoplasty, and an array of surgeons’ variations have come about. Ferreira et al. have proposed a classification for dorsal preservation rhinoplasty that splits it into foundation techniques and surface techniques. Foundation techniques are all techniques with impaction osteotomies – push or letdown; in this group, there is always a more minor or significant disruption between the skull and the nasal pyramid. Surface techniques are techniques that work on the surface of the nose (like the traditional structured approaches); in this group, there are no impaction osteotomies, so there is no disruption between the skull and the nose.
Sedation Weaning Initiative Targeting Methadone Exposure: Single Center Improvements in Withdrawal Symptoms and Hospital Length of Stay for Pediatric Cardiac Critical Care
Authors: King Caitlin E. , Wood Danielle N. , Koo Jeannie , Cutler Austin B. , Vesel Travis P. 
Published: April 2023

Objectives:
Sedation and pain medications are necessary in the management of postoperative pediatric cardiac patients. Prolonged exposure to these medications can lead to negative side effects including withdrawal. We hypothesized that standardized weaning guidelines would decrease exposure to sedation medications and decrease withdrawal symptoms. The primary aim was to decrease average days of methadone exposure to within goal for moderate- and high-risk patients within 6 months.

Design:
Quality improvement methods were used to standardize sedation medication weaning in a pediatric cardiac ICU.

Setting:
This study took place at Duke Children’s Hospital Pediatric Cardiac ICU in Durham, North Carolina from January 1, 2020, to December 31, 2021.

Patients:
Children less than 12 months old admitted to the pediatric cardiac ICU who underwent cardiac surgery.

Interventions:
Sedation weaning guidelines were implemented over the course of 12 months. Data were tracked every 6 months and compared with the 12 months pre-intervention. Patients were stratified into low, moderate, and high risk withdrawal categories based on duration of opioid infusion exposure.

Measurements and Main Results:
Total sample size was 94 patients in the moderate and high risk categories. Process measures included documentation of Withdrawal Assessment Tool scores and appropriate methadone prescription in patients which increased to 100% post-intervention. For outcome measures, we observed decreased dexmedetomidine infusion duration, decreased methadone wean duration, decreased frequency of elevated Withdrawal Assessment Tool scores, and decreased hospital length of stay post-intervention. For the primary aim, methadone wean duration consistently decreased after each study period. Our intervention did not adversely impact balancing measures.

Conclusions:
A quality improvement initiative to standardize sedation weaning in a Pediatric Cardiac ICU was successfully implemented and was correlated with decreased duration of sedation medications, decreased withdrawal scores, and decreased length of stay.
Real-Time Augmented Reality Annotation for Surgical Education During Laparoscopic Surgery: Results from a Single-Center Randomized Controlled Trial and Future Aspects
Authors: Huettl Florentine , Heinrich Florian , Boedecker Christian , Vradelis Lukas , Ludt Annekathrin , Kneist Werner , Lang Hauke , Hansen Christian , Huber Tobias 
Published: April 2023

Background:
We developed an interactive augmented reality tool (HoloPointer) that enables real-time annotation on a laparoscopy monitor for intraoperative guidance. This application operates exclusively via verbal commands and head movements to ensure a sterile workflow.

Study Design:
Purpose of this randomized controlled clinical trial was to evaluate the integration of this new technology into the operating room. This prospective single-center study included 32 elective laparoscopic cholecystectomies (29 surgical teams, 15 trainees, 13 trainers). Primary objectives and assessment measures was the HoloPointer’s influence on surgical performance (subjective assessment, global operative assessment of laparoscopic skills - GOALS, and Critical View of Safety -CVS). Secondary objectives and outcome variables were its influence on operation time, quality of assistance (5 point likert-scale), and user-friendliness (System Usability Scale - SUS, 0-100 points).

Results:
Gestural corrections were reduced by 59.4% (4.6 SD 8.1 vs. 1.9 SD 4.7; p > 0.05) and verbal corrections by 36.1% (17.8 SD 12.9 vs. 11.4 SD 8.1; p > 0.05). Subjective surgical performance could be improved by 84,6% of participants. No statistically significant differences were observed for objective parameters GOALS, CVS and operation time. In the SUS, the application achieved an average score of 72.5 SD 16.3 (good user-friendliness). Of the participants, 69.2% wanted to use the HoloPointer more frequently.

Conclusion:
The majority of trainees had improved their surgical performance using the HoloPointer in elective laparoscopic cholecystectomies, and the rate of classic but potentially misleading corrections was noticeably reduced. The HoloPointer has the potential to improve education in minimally invasive surgery.