bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of the ferroptosis-related STEAP3 gene is a specific predictor of poor triple-negative breast cancer patient outcomes
Authors: Yuan Lifang , Liu Jiannan , Bao Lei , Qu Huajun , Xiang Jinyu , Sun Ping 
Published: March 2023

ObjectiveThis study was designed to assess ferroptosis regulator gene (FRG) expression patterns in patients with TNBC based on data derived from The Cancer Genome Atlas (TCGA). Further, it was utilized to establish a TNBC FRG signature, after which the association between this signature and the tumor immune microenvironment (TIME) composition was assessed, and relevant prognostic factors were explored.MethodsThe TCGA database was used to obtain RNA expression datasets and clinical information about 190 TNBC patients, after which a prognostic TNBC-related FRG signature was established using a least absolute shrinkage and selection operator (LASSO) Cox regression approach. These results were validated with separate data from the Gene Expression Omnibus (GEO). The TNBC-specific prognostic gene was identified via this method. The STEAP3 was then validated through Western immunoblotting, immunohistochemical staining, and quantitative real‐time polymerase chain reaction (RT-qPCR) analyses of clinical tissue samples and TNBC cell lines. Chemotherapy interactions and predicted drug sensitivity studies were investigated to learn more about the potential clinical relevance of these observations.ResultsThese data revealed that 87 FRGs were differentially expressed when comparing TNBC tumors and healthy tissue samples (87/259, 33.59%). Seven of these genes (CA9, CISD1, STEAP3, HMOX1, DUSP1, TAZ, HBA1) are significantly related to the overall survival of TNBC patients. Kaplan-Meier analyses and established FRG signatures and nomograms identified CISD1 and STEAP3 genes of prognostic relevance. Prognostic Risk Score values were positively correlated with the infiltration of CD4+ T cells (p = 0.001) and myeloid dendritic cells (p =0.004). Further evidence showed that STEAP3 was strongly and specifically associated with TNBC patient OS (P&lt;0.05). The results above were confirmed by additional examinations of STEAP3 expression changes in TNBC patient samples and cell lines. High STEAP3 levels were negatively correlated with half-maximal inhibitory concentration (IC50) values for GSK1904529A (IGF1R inhibitor), AS601245 (JNK inhibitor), XMD8−85 (Erk5 inhibitor), Gefitinib, Sorafenib, and 5-Fluorouracil (P &lt; 0.05) in patients with TNBC based on information derived from the TCGA-TNBC dataset.ConclusionIn the present study, a novel FRG model was developed and used to forecast the prognosis of TNBC patients accurately. Furthermore, it was discovered that STEAP3 was highly overexpressed in people with TNBC and associated with overall survival rates, laying the groundwork for the eventually targeted therapy of individuals with this form of cancer.
Rev-erbα agonist SR9009 protects against cerebral ischemic injury through mechanisms involving Nrf2 pathway
Authors: Sheng Mingyue , Chen Xun , Yu Yan , Wu Qi , Kou Junping , Chen Gangling 
Published: March 2023

Backgrounds: The circadian clock protein Rev-erbα is a crucial regulator of circadian rhythms that affects multiple molecular, cellular, and physiology pathways that control susceptibility, injury, and recovery in the neurological disorders. Emerging evidence suggest that Rev-erbα plays a key role in the inflammation and oxidative stress, two pivotal mechanisms in the pathogenesis, progression, and recovery process of ischemic stroke. However, it remains inconclusive whether Rev-erbα activation is protective against ischemic brain damage. Nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, a master regulator of inflammatory and oxidative responses. Our study aimed to determine whether pharmacological activation of Rev-erbα by SR9009 protects against acute ischemic brain damage partly via Nrf2 pathway.Methods: Adult mice were pretreated with SR9009 or Nrf2 inhibitor all-trans-retinoic acid (ATRA) for 3 days prior to Sham or middle cerebral artery occlusion (MCAO) operation. After ischemia for 1 h and reperfusion for 24 h, the neurological function and cerebral infarction volume were determined, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and glutathione peroxidase (GSH-PX) activity in serum were detected by kit. The mRNA and/or protein level of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), Period (Per)1, Brain and muscle arnt-like1 (Bmal1), Circadian locomotor output cycles kaput (Clock), Rev-erbα, Nrf2, heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (NQO1) in cerebral cortex were detected by q-PCR and Western blot.Results: We confirmed that SR9009 activated Rev-erbα gene in the cerebral cortex under basal condition. At 24 h after reperfusion, SR9009 ameliorated acute neurological deficits, reduced infarct volume. Meanwhile, the inflammatory TNF-α, IL-1β, iNOS and MDA content levels were significant decreased, SOD and GSH-PX activity were obviously increased, which were markedly blunted (or abolished) by ATRA. SR9009 enhanced the induction of Nrf2 and its downstream target genes HO-1 and NQO1 after ischemic insult. In addition, we found that SR9009 restored Rev-erbα, Bmal1, Clock, Per1 genes expression in the cerebral cortex under ischemic condition.Conclusion: Taken together, Rev-erbα activation by SR9009 protects against ischemic stroke damage, at least, partly through Nrf2 pathway.
Impact of selenium biofortification on production characteristics of forages grown following standard management practices in Oregon
Authors: Hall Jean A. , Bobe Gerd , Filley Shelby J. , Bohle Mylen G. , Pirelli Gene J. , Wang Guogie , Davis T. Zane , Bañuelos Gary S. 
Published: March 2023

IntroductionLow selenium (Se) concentrations in soils and plants pose a health risk for ruminants consuming locally-grown forages. Previous studies have shown that Se concentrations in forages can be increased using soil-applied selenate amendments. However, the effects of foliar selenate amendments applied with traditional nitrogen-phosphorus-potassium-sulfur (NPKS) fertilizers on forage yields, and nutrient contents, and agronomic efficiencies are unknown.MethodsUsing a split plot design, we determined the effects of springtime sodium selenate foliar amendment rates (0, 45, and 90 g Se ha-1) and NPKS application (none, NPK for grasses/PK for alfalfa, and NPKS/PKS fertilization at amounts adapted to meet local forage and soil requirements) on forage growth and N, S, and Se concentrations, yields, and agronomic efficiencies. This 2-year study was conducted across Oregon on four representative forage fields: orchardgrass (Dactylis glomerata L.) in Terrebonne (central Oregon), grass-clover mixture in Roseburg (southwestern Oregon), and both grass mixture and alfalfa (Medicago sativa L.) fields in Union (eastern Oregon).ResultsGrasses grew poorly and were low in N content without NPK fertilization. Fertilization with NPK/PK promoted forage growth, increased forage N concentrations, and had to be co-applied with S when plant available S was low. Without Se amendment, forage Se concentrations were low and further decreased with NPKS/PKS fertilization. Selenate amendment linearly increased forage Se concentration without adversely affecting forage yields, N and S concentrations, or N and S agronomic efficiencies.DiscussionImportantly, S fertilization did not interfere with Se uptake in Se amended plots. In conclusion, co-application of NPKS/PKS fertilizers and foliar sodium selenate in springtime is an effective strategy to increase forage total Se concentrations, while maintaining optimal growth and quality of Oregon forages.
Neuropsychological monitoring of cognitive function and ICF–based mental components in patients with malignant brain tumours
Authors: Pilarska Agnieszka , Pieczyńska Anna , Hojan Katarzyna 
Published: March 2023

BackgroundCognitive deficits are one of the important clinical features of patients with brain tumours, which can affect up to 30–90% of patients before treatment. The consequence is a significant and rapid degradation of the patient’s intellectual functioning, seizures, paralysis and other symptoms that prevent independent functioning. This results in a reduced quality of life and a psychological crisis not only for the patient but also for their relatives. Maintaining the patient’s function at the highest level for as long as possible is particularly important, given that long-term remission or a cure is unlikely or accompanied by significant disability.PurposeThis paper aims to provide a narrative review to the neuropsychological procedure for monitoring cognitive function in patients with brain tumours, which may be helpful in developing adequate clinical practice and appropriate management procedures.MethodsA narrative review was applied to search broadly across disciplines, retrieving literature from several databases (PubMed, Web of Science, and EBSCOhost).Results(1) discussing the methodological aspects of neuropsychological tools for monitoring cognitive function in brain tumour patients, (2) identifying the most commonly used tools and (3) their practical applicability according to the cognitive function components of the International Classification of Functioning, Disability and Health (ICF).ConclusionThis article points to the need to systematise research tools or develop new ones, adapted to diagnostic needs with high psychometric characteristics, with particular attention to memory processes and learning effect. Rehabilitation of patients is also an important issue, which requires the use of adequate tools to assess functional disability. The International Classification of Functioning, Disability and Health (ICF) seems to be useful in this respect. The ICF has the advantage of targeting actions to improve the condition of the individual and to keep them as long as possible in a state of well-being that allows them to function effectively in society or to return to work. This is particularly important in view of the ageing population and the increasing number of diagnoses related to brain tumours.
Strategies for reducing meat consumption within college and university settings: A systematic review and meta-analysis
Authors: Chang Kenjin B. , Wooden Alyssa , Rosman Lori , Altema-Johnson Daphene , Ramsing Rebecca 
Published: March 2023

IntroductionDespite the considerable public and planetary health benefits associated with reducing the amount of meat consumed in high-income countries, there is a limited empirical understanding of how these voluntary changes in food choice can be effectively facilitated across different settings. While prior reviews have given us broad insights into the varying capacities of behavior change strategies to promote meaningful reductions in meat consumption, none have compared how they perform relative to each other within a uniform dining context.MethodsTo address this gap in the literature, we synthesized the available research on university-implemented meat reduction interventions and examined the variations in the success rates and effect estimates associated with each of the three approaches identified in our systematic review.ResultsFrom our analyses of the 31 studies that met our criteria for inclusion (n = 31), we found that most were successful in reducing the amount of meat consumed within university settings. Moreover, independent of the number of individual strategies being used, multimodal interventions were found to be more reliable and effective in facilitating these changes in food choice than interventions targeting the choice architecture of the retail environment or conscious decision-making processes alone.DiscussionIn addition to demonstrating the overall value of behavior change initiatives in advancing more sustainable dining practices on college and university campuses, this study lends further insights into the merits and mechanics underlying strategically integrated approaches to dietary change. Further investigations exploring the persistence and generalizability of these effects and intervention design principles are needed.Systematic review registrationhttps://doi.org/10.17605/OSF.IO/DXQ5V, identifier: 10.17605/OSF.IO/DXQ5V.
Infection Prevalence of Microsporidia Vairimorpha (Nosema) spp. in Japanese Bumblebees
Authors: Yanagisawa Takahiro , Kato Yuto , Inoue Maki N. 
Published: March 2023

Microsporidia are spore-forming intracellular parasites of various invertebrates and vertebrates. Vairimorpha bombi negatively affects the fitness of bumblebees and its prevalence correlates with declining bumblebee populations. The invasive alien species Bombus terrestris colonized Japan and possibly introduced new parasites. To assess the infection prevalence of V. bombi in Japanese bumblebees and B. terrestris, we investigated V. bombi infections using PCR and microscopy. The prevalence of sporulating V. bombi infections in three Bombus s. str. species/subspecies was low, whereas that of non/low-sporulating Vairimorpha sp. infections in three Diversobombus species/subspecies was high. Invasive B. terrestris showed low prevalence of non/low-sporulating V. bombi infections and shared the same V. bombi haplotype with B. hypocrita found in Hokkaido, where B. terrestris is present, and in Honshu, where B. terrestris is absent. Although V. bombi may have been introduced with B. terrestris colonies imported from Europe, it seems to be originally distributed in Japan. Furthermore, a new Vairimorpha sp. was found in Japanese bumblebee species. V. bombi and Vairimorpha sp. showed different organ and host specificities in bumblebees. There are no reports on the specific effects of other Vairimorpha spp. on bumblebees; further studies are needed to clarify the individual characteristics of Vairimorpha spp.
Acoustic Backscatter Communication and Power Transfer for Batteryless Wireless Sensors
Authors: Oppermann Peter , Renner Bernd-Christian 
Published: March 2023

Sensors for industrial and structural health monitoring are often in shielded and hard-to-reach places. Acoustic wireless power transfer (WPT) and piezoelectric backscatter enable batteryless sensors in such scenarios. Although the low efficiency of WPT demands power-conserving sensor nodes, backscatter communication, which consumes near-zero power, has not yet been combined with WPT. This study reviews the available approaches to acoustic WPT and active and passive acoustic through-metal communication. We design a batteryless and backscattering tag prototype from commercially available components. Analysis of the prototypes reveals that low-power hardware poses additional challenges for communication, i.e., unstable and inaccurate oscillators. Therefore, we implement a software-defined receiver using digital phase-locked loops (DPLLs) to mitigate the effects of oscillator instability. We show that DPLLs enable reliable backscatter communication with inaccurate clocks using simulation and real-world measurements. Our prototype achieves communication at 2 kBs−1 over a distance of 3 m. Furthermore, during transmission, the prototype consumes less than 300 μW power. At the same time, over 4 mW of power is received through wireless transmission over a distance of 3 m with an efficiency of 2.8%.
Auto-Calibrated Charge-Sensitive Infrared Phototransistor at 9.3 µm
Authors: Bahrehmand Mohsen , Gacemi Djamal , Vasanelli Angela , Li Lianhe , Davies Alexander Giles , Linfield Edmund , Sirtori Carlo , Todorov Yanko 
Published: March 2023

Charge-sensitive infrared photo-transistors (CSIP) are quantum detectors of mid-infrared radiation (λ=4 µm−14 µm) which have been reported to have outstanding figures of merit and sensitivities that allow single photon detection. The typical absorbing region of a CSIP consists of an AlxGa1-xAs quantum heterostructure, where a GaAs quantum well, where the absorption takes place, is followed by a triangular barrier with a graded x(Al) composition that connects the quantum well to a source-drain channel. Here, we report a CSIP designed to work for a 9.3 µm wavelength where the Al composition is kept constant and the triangular barrier is replaced by tunnel-coupled quantum wells. This design is thus conceptually closer to quantum cascade detectors (QCDs) which are an established technology for detection in the mid-infrared range. While previously reported structures use metal gratings in order to couple infrared radiation in the absorbing quantum well, here, we employ a 45° wedge facet coupling geometry that allows a simplified and reliable estimation of the incident photon flux Φ in the device. Remarkably, these detectors have an “auto-calibrated” nature, which enables the precise assessment of the photon flux Φ solely by measuring the electrical characteristics and from knowledge of the device geometry. We identify an operation regime where CSIP detectors can be directly compared to other unipolar quantum detectors such as quantum well infrared photodetectors (QWIPs) and QCDs and we estimate the corresponding detector figure of merit under cryogenic conditions. The maximum responsivity R = 720 A/W and a photoconductive gain G~2.7 × 104 were measured, and were an order of magnitude larger than those for QCDs and quantum well infrared photodetectors (QWIPs). We also comment on the benefit of nano-antenna concepts to increase the efficiency of CSIP in the photon-counting regime.
Patient Satisfaction with Pharmaceutical Services in Jordan: A Cross-Sectional Study
Authors: Amara Noor , Naser Abdallah Y. , Taybeh Esra’ O. 
Published: March 2023

Objectives: Pharmacists’ responsibilities have become more patient-oriented so exploring patients’ satisfaction would lead to identifying and improving the quality of pharmaceutical services. The aim of the present study was to assess patients’ satisfaction with pharmaceutical services in Jordan
Methods: A cross-sectional study was conducted between December 2018 and April 2019. Patients’ satisfaction was assessed using three subscales; managing therapy, interpersonal relationship, and general satisfaction, in addition, to the socio-demographic questionnaire. Participants were asked about the degree of their satisfaction with the pharmaceutical services in all subscales’ questions using a 5-point Likert scale.
Results: A total of 1,333 patients participated in the study. The mean patient age was 32.4 years (SD = 11.6)52.5% (n = 700) were female. Patients had a good satisfaction score for overall pharmaceutical services with a mean score of 51.4 ± 11.4 out of 70. Among the subscales, the interpersonal relationship subscale showed a satisfaction score of 75.7% while the other two subscales (general satisfaction and managing therapy) showed slightly lower scores with 75.0% and 69.6%, respectively. Having good health and governmental or military health insurance, visiting the pharmacy more frequently, and dispensing medications from governmental hospitals, primary healthcare centers, or military hospital pharmacies were important predictors of better patient satisfaction with pharmaceutical services (p < 0.05).
Conclusions: This study presents some nationwide patient-reported outcomes about patient satisfaction in general pharmacy settings in Jordan. Focusing on patients’ satisfaction while providing pharmaceutical services is needed.
Identification of Pharmaceutically Important Constituents of Quinoa Root
Authors: Khan Iqra Haider , Javaid Arshad 
Published: March 2023

The present investigation was carried out to explore the bioactive compounds from the n-hexane fraction of methanolic extract of quinoa (Chenopodium quinoa Willd.) roots. For this purpose, C. quinoa roots were collected, shade dried, and crushed into a fine powder. The powdered material was extracted in methanol, filtered, and the filtrate was partitioned with n-hexane, followed by GC-MS analysis of the n-hexane fraction. The quantitative determination of this fraction revealed the presence of 15 phytochemical constituents of diverse nature. Among these, octadec-9-enoic acid (44.18%); n-hexadecanoic acid (18.87%); methyl (Z)-octadec-9-enoate (12.87%); methyl hexadecanoate (4.30%); 2,3-dihydroxypropyl elaidate (3.63%); phthalic acid (3.08%); methyl octadecanoate (2.27%) and 1,12-tridecadiene (2.00%) were prevailing as the most abundant to moderately occurring compounds. A thorough literature survey was carried out to collect information regarding the pharmaceutical properties of the identified compounds. It showed some of the identified compounds namely dodecanoic acid; tetradecanoic acid; 2-benzoyl-d-galactosan; n-hexadecanoic acid; methyl octadecanoate; octadec-9-enoic acid; and 2,3-dihydroxypropyl elaidate possess antifungal, antibacterial, antioxidant, antiviral, anti-inflammatory, and/or anti-cancer properties.
Development of n-type Zn(O, S) buffer layer deposited by open-air CVD method for Cu(In, Ga)Se2 solar cells
Authors: Funaki Akihiro , Furumaki Fumiya , Nishimura Takahito , Yamada Akira 
Published: March 2023

Abstract
A Zn(O, S) thin-film is deposited utilizing an open-air CVD method by evaporating zinc-diethyldithiocarbamate, which is a non-vacuum and dry process. In an X-ray diffraction measurement, it is revealed that the films have a wurtzite structure and an [O]/([O] + [S]) ratio of 10%. A bandgap energy of 3.1 eV is estimated from the transmittance and reflectance spectra. By applying the Zn(O, S) as an n-type buffer layer, Cu(In, Ga)Se2 solar cells are fabricated. In the current density–voltage characteristics, distortion is observed at the bias voltages above the open-circuit voltage. It is implied that a large conduction band offset exists at a Zn(O, S)/CIGS interface. A quantum efficiency spectrum in the wavelength region of 380–512 nm is improved compared to a traditional CdS buffer layer. Finally, a 9.2%-efficient CIGS solar cell is demonstrated utilizing the Zn(O, S) buffer layer through an all-dry process.
تكثيف المشاهد والصور ودلالته في سورة القمر
Authors: عباس وجدان صالح 
Published: March 2023

يتناول البحث الموسوم ب( تكثيف المشاهد والصور ودلالته في سورة القمر ) الحديث عن التكثيف الذي هيمن على سورة القمر من حيث تصوير مشاهد العذاب برعب وخوف وعنف شهدته أغلب مقاطع السورة ولقطاتها الخاطفة السريعة , وتركزت من خلاله أبعاد الصورة الفنية البيانية فورد التكثيف مكللا بالدلالات والايحاءات من حلال التشبيه وكيفيته , والكناية وأبعادها, والتي تراوحت بين الايجاز الشديد للمشاهد وتداعياتها بغرض توعية النفس الغافلة ورشادها , بعرض يضم مشاهد الدنيا بين دفتيه وينعقد على مشهد من مشاهد يوم القيامة افتحت به السورة , وآخر ختمت به , وانتهت المشاهد بصورة النعيم وجزاء المتقين بجلسة المقربين المكرمين ومن الله التوفيق.
Some Classes of Phytoplankton in Iraqi waters
Authors: Ameen Merza Feryal , Abul-Doanej Hasan Azaati Ibadi Abul-Doanej 
Published: March 2023

This review aimed to survey some classes of phytoplankton composition in Iraqi waters. These data were taken from investigations of phytoplankton in Iraq, covering both freshwater and saline waters in many cities. A total of 342 taxa belonging to twelve classes have been recorded in the review. Among these classes, Chlorophyceae presented a larger number of taxa, while Compsopogonophyceae, Conjugatophyceae, Coscinodiscophyceae and Trebouxiophyceae presented one taxon only. This study proposes to present a checklist of phytoplanKton in Iraq, built on the compilation of preceding surveys.