bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative bioinformatics approaches to identify novel molecular gene expression signatures and pathways of Cystic Fibrosis (CF)
Authors: Mia Mamun , Oany Arafat Rahman , Bhowmik Mousumi , Pervin Tahmina , Duza Syeda Sharmin 
Published: June 2023

Abstract
Cystic Fibrosis (CF) is the most common lethal autosomal recessive disorder characterized by the defective function of the CF transmembrane conductance regulator (CFTR) gene. Worldwide, the incidence ratio of CF is approximately 1:3000 in live births per annum. Recently, the number of adult patients has been reported to outnumber the number of pediatric sufferers. Though the diagnosis and treatment are available for CF patients’ corrective treatment for non-specific mutations, patients with rare mutations, advances in personalized biomarkers, and whether other genes and signaling pathways are related to CF are being investigated and they are also in the early stage of development and validation. In the present study, we have employed systems biology approaches to retrieve the potential biomarkers and pathways from transcriptomic profiling. Initially, we identified 104 up-regulated and 107 down-regulated genes from a total of 54676 differentially expressed genes. The up-regulatory genes mainly concentrate on Glutamatergic synapses, and the down-regulatory genes are on Ubiquitin mediated proteolysis. The involved pathways associated with these genes were also assessed through pathway enrichment, and we mainly focused on different pathways related to bronchial epithelial cells. After the subsequent enrichment of these genes, we have identified 6 hub genes, WWP2; RNASEL; CUL1; CDC42; HDAC4, and UBA2, which play a crucial role in CF and are verified by expression profile analysis. Our study found that the WWP2 gene in CF, as reported here for the first time.
Accessory infraspinatus muscle in a male cadaver: a case report
Authors: Cobb Savannah E. , Palmer Stephanie M. , Cooke Siobhán B. 
Published: June 2023

Abstract
Purpose
The infraspinatus muscle is a component of the rotator cuff that has relatively few known variants. Understanding variation in this structure is critical for clinicians and may have implications for understanding human development.
Methods
One 83-year-old male cadaver was dissected as part of the graduate anatomy education curriculum at Johns Hopkins University School of Medicine in fall 2022. Dissections were guided using Grant’s Dissector, 17th edition as a reference.
Results
An accessory infraspinatus muscle as well as other muscular and neurological variations were discovered in the cadaver. The variant muscle is thin and flat with parallel fibers originating on the medial dorsal surface of the scapula, running under the scapular spine, and inserting on the greater tubercle of the humerus. The accessory infraspinatus muscle is separated from the infraspinatus muscle and deltoid muscle by fascial planes, and its muscle body extends past the tendinous portion of the underlying infraspinatus muscle; we suggest these as additional criteria for the identification of the accessory infraspinatus.
Conclusion
This is the second reported occurrence of an accessory infraspinatus muscle and the first known occurrence in a male. The co-occurrence of this rare variant with other variations in the limbs, back, and head suggests that variation in development may be affecting the entire body, ultimately being responsible for the presence of an accessory infraspinatus in this cadaver.
Improved wear resistance of 440C steel balls through mechanical alloying with Al 2 O 3 powders
Authors: Liu Zhihui , Zhang Zhijian , Zhang Jiahao , Wen Xue , Zhu Kejun , Chen Zhigang 
Published: June 2023

Abstract
This study investigates the effect of mechanical ball milling with Al2O3 powders on the wear resistance of 440C steel balls. A specially-designed grinding jar was employed for the mechanical ball milling process, allowing for the comparison of samples treated with Al2O3 powders and without Al2O3 powders, as well as the heat-treatment samples. The roughness, microhardness, residue stress, and wear resistance of treated samples were investigated and compared. The results reveal that the addition of Al2O3 powders leads to significant grain refinement, increased dislocation density, and the presence of nano-structured staking faults. The treated samples exhibit a strengthened layer exceeding 200 µm, with maximum microhardness reaching 753 HV and residual stress measuring − 237 MPa. Furthermore, the treated samples demonstrate reduced coefficient of friction, wear volume, and improved wear resistance compared to the other samples. These findings highlight the efficiency of mechanical ball milling with Al2O3 powders in enhancing the wear resistance of ball mechanical components, thereby offering potential benefits for industrial applications.
Another approach to treat large renal pelvis calculi: presetting a double J tube and ureteroscopic lithotripsy through ureteral access sheath
Authors: Yang Fan , Zhuifeng Guo , Hang Wang , Jiawen Wu , Xuwei Lu 
Published: June 2023

Abstract
Objective: To evaluate a new approach to treat renal pelvis calculi. Patients with large renal pelvis calculi who were deemed inappropriate to undergo percutaneous nephrolithotomy (PCNL) were treated by presetting a double J tube and ureteroscopic lithotripsy through ureteral access sheath (UAS).
Methods: We retrospectively reviewed clinical data from 130 cases where patients had 2-3cm renal pelvis calculi with mild hydronephrosis (separation of renal pelvis ≤20mm) from January 2017 to January 2022 and divided them into three groups. In group A, 36 patients were treated by presetting a double J tube as stage I surgery, rigid ureteroscopic lithotripsy (URL) and flexible ureteroscopic lithotripsy (FURL) through ureteral access sheath as stage II surgery. In this group, URL was mainly used to break calculi and FURL was occasionally used to seek smaller calculi if required. In group B, 31 patients were treated URL alone. In group C, 63 patients were treated with single-tract mini-PCNL. The calculi clearance rate, operation time, hospitalization days, hospital costs and complications between the groups were compared.
Results: The calculi clearance rate in group A was 80.56%, group B 74.19% and group C 84.13%, and the difference was not statistically significant (P>0.05). The operation time of group A was (93.94±14.22) min, group B (104.87±13.42) min and group C (82.63±18.39) min, and there were significant differences between group B and both group A and group C (P<0.05). The hospitalization days of group A were (2.63±0.58) d, group B (3.47±1.01) d, and group C (4.58±1.58) d, and there were significant differences between group A and both group B and group C (P< 0.05). In group C, there were 2 cases of bleeding which were remedied with conservative treatment.
Conclusion: For patients with 2-3cm renal pelvis calculi and mild hydronephrosis, the treatment of presetting a double J tube and ureteroscopic lithotripsy through ureteral access sheath has the advantages of less trauma, less hospitalization days and a lower complication rate. The new method has reliable calculi clearance rate, higher lithotripsy efficiency and lower economic cost than FURL alone.
FDX1 promotes elesclomol-induced PANoptosis in diffuse large B-cell lymphoma by activating IRF3/IFN-β signaling
Authors: Wang QI , Chen Weifeng , Liu Dandan , Feng Xiaoting , Chen Yiqiu , Zhang Lantian , Lu Di , Zeng Jun , Zhang Xiaoren , Jiang Yuhang 
Published: June 2023

Abstract
Background
The identification of cuproptosis, a newly defined form of copper-induced cell death, has provided innovative insights for cancer therapy. However, the function and regulatory mechanism of cuproptosis have not been elucidated in diffuse large B-cell lymphoma (DLBCL), which remains a major clinical challenge and requires the development of new therapeutic approaches.
Methods
By utilizing databases we analyzed the correlation between FDX1 expression and overall survival of DLBCL patients. We performed cell viability assay, 7-AAD/EdU staining and subsequent flow cytometry to explore the role of FDX1 in DLBCL cell apoptosis, cell proliferation and elesclomol (ES)-induced cell death in vitro. Then in vivo FDX1 function was confirmed through animal models. To investigate the underlying mechanisms, RNA-seq, RT-qPCR, Westernblot and tissue microarray (TMA) assays were carried out.
Results
We found that loss of the mitochondrial matrix reductase FDX1 in DLBCL cells impairs the antitumor effect of elesclomol (ES), which performs its function by transporting excess copper into cells. Overexpressing (OE) FDX1 significantly sensitized DLBCL cells to ES-induced cell death in vitro and enhanced the anticancer activity of ES in vivo. Mechanistically, ES-induced PANoptosis in FDX1-OE cells is dependent on IFN-β. FDX1 promotes the induction of IFN-β by increasing IRF3 phosphorylation in DLBCL cells upon ES treatment. Consistent with this finding, patient cohort analysis revealed that FDX1 expression correlated positively with enhanced IRF3 phosphorylation.
Conclusions
Our findings are the first to identify the central role of FDX1 in synergizing with ES to activate IFN-β signaling and induce PANoptosis. This knowledge enables us to re-explore the clinical anticancer potential of ES as a novel therapeutic strategy for DLBCL.
A simple and efficient method for loess separation and composition analysis
Authors: Zhao Shenglan , Huang Peng , Zhou Meiling , Yang Hua , Wang Qianqian , Wang Rongmin , Wang Zeyuan , He Yufeng 
Published: June 2023

Abstract
Loess is a widely existing cheap natural inorganic material, due to its complex composition and insufficient understanding of its efficient utilization and repeated utilization, it has not been widely used. Therefore, in this article, a simple method with buoyancy and gravity sedimentation to separate loess into large, medium and small particles. Besides, the main components of different particle size loess particles were provided. The separated particles were characterized by Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), Brunauer-EmmettTeller (BET), Thermal gravity (TG), X-ray diffraction (XRD), X-ray fluorescence spectrum (XRF), and X-ray photoelectron spectroscopy (XPS). It was found that the mineral compositions in large, medium and small loess particles vary with particle size. The large loess particles contains more aluminosilicate, medium loess particles contains both aluminosilicate and clay. It can play different roles according to the content of different particle sizes of loess particles, and realize the efficient use of loess particles. The main component of small loess particles is clay. Clay particles have a wide range of applications and can be used for adsorption as well as catalytic degradation of pollutants as a catalyst carrier. Therefore, it is necessary to separate loess by a simple method to improve its utilization rate.
Itaconate enhances oncolytic virotherapy by multitarget inhibition of antiviral and inflammatory pathways
Authors: Kurmasheva Naziia , Said Aida , Wong Boaz , Kress Alena , Liu Zhenlong , Wang Chen , Carter-Timofte Madalina , Holm Emilia , Horst Demi van der , Hoang Huy-Dung , Twayana Krishna , Ottosen Rasmus , Svenningsen Esben , Begnini Fabio , Kiib Ander , Weiss Hauke , Carlo Daniele Di , Muscolini Michela , Higgins Maureen , Kinderman Priscilla , Heijden Mirte van der , Tong Tong , Ozsvar Attila , Hou Wen-Hsien , Schack Vivien , Holm Christian , Montfoort Nadine van , Zheng Yunan , Ruzek Melanie , Kalucka Joanna , Vega Laureano de la , Elgaher Walid , Korshoej Anders , Lin Rongtuan , Hiscott John , Poulsen Thomas , O'Neill Luke , Diallo Jean-Simon , Farin Henner , Alain Tommy , Olagnier David 
Published: June 2023

Abstract

Altered metabolism and defective innate immune responses are hallmarks of tumor cells, creating a niche that can be exploited by viruses with oncolytic properties. However, heterogeneity in responses to oncolytic virotherapy is a barrier to clinical effectiveness. Resistance to oncolytic virotherapy exists and occurs via inhibition of viral spread within the tumor that may result in treatment failures. Here we show that the Krebs cycle-derived metabolite itaconate and its chemical derivatives or natural isomers enhance oncolytic virotherapy with VSVΔ51M in various models including resistant cancer cell lines, murine tumor biopsies, three-dimensional (3D) patient-derived colon tumoroids and organotypic brain tumor slices. The strongest sensitizing effect to VSVΔ51M infection within the itaconate family is elucidated by 4-octyl itaconate (4-OI), a known activator of nuclear factor erythroid 2-related factor 2 (NRF2). Importantly, the sensitization to VSVΔ51M with 4-OI is not observed in non-cancer primary human cells, healthy mouse-derived tissues, and normal human colon organoids. Additionally, 4-OI improves the virus spread within a resistant mouse colon tumor model in vivo. Mechanistically, we found that the effect of 4-OI is not mediated by NRF2 or KEAP1. Instead, we show that 4-OI targets multiple pathways including the RIG-MAVS, the IKKβ-NF-κB and the JAK1-STAT1 pathways through the modification of cysteine residues in MAVS, IKKβ and JAK1, respectively. Here, we propose that the combination of a metabolite-derived drug with an oncolytic virus agent can greatly improve anticancer therapeutic outcomes by direct interference with the type I IFN and NF-κB-mediated antiviral responses.
Frictional mechanics of knots
Authors: Leuthäusser Ulrich 
Published: June 2023

Abstract
For some important knots closed-form solutions are presented for the holding forces which are needed to keep a knot in equilibrium for given pulling forces. If the holding forces become zero for finite pulling forces, the knot is self-locking and is called stable. This is only possible when first, the friction coefficient exceeds a critical value and second, when there is additional pressure on some knot segments sandwiched by surrounding knot segments. The number of these segments depends on the topology of the knot and is characteristic for it. The other important parameter is the total curvature of the knot. In this way, the complete frictional contact inside the knot is taken into account. The presented model can explain the available experiments.
High Dietary Inflammatory Index associates with inflammatory proteins in plasma
Authors: Mattavelli Elisa , Piperini Elisa , Asnicar Francesco , Redaelli Laura , Grigore Liliana , Pellegatta Fabio , Nabinejad Amir , Tamburini Sabrina , Segata Nicola , Catapano Alberico Luigi , Baragetti Andrea 
Published: June 2023

Abstract
Background and aim:
Unhealthy dietary habits and highly caloric foods induce metabolic alterations and promote the development of inflammatory consequences of obesity and insulin resistance, which are epidemic conditions leading to diabetes and cardiovascular diseases. Describing an inflammatory effect of diet is difficult to pursue, owing to the lack of quali-quantitative dietary assessment standardization. The Dietary Inflammatory Index (DII) has been proposed as an estimator of the pro- or anti-inflammatory effect of nutritional components. Higher DII values, which indicate an increased intake of nutrients with pro-inflammatory effects, relates to an increased risk of metabolic and cardiovascular diseases in epidemiological studies. Whether higher DII values reflect biologically relevant variations of inflammatory proteins in plasma, has been poorly described today.
Methods:
In this cross-sectional study, seven-days dietary records from 663 subjects in primary prevention for cardiovascular diseases were analyzed to derive the intake of nutrients, foods and to calculate DII. To associate DII with the Normalized Protein eXpression (NPX), an index of abundance, of a targeted panel of 368 inflammatory biomarkers (Olink™) measured in the plasma, we divided the population by the median value of DII (1.60 (0.83–2.30)).
Results:
332 subjects with estimated DII over the median value reported a higher intake of saturated fats but lower intakes of poly-unsaturated fats, including omega-3 and omega-6 fats, versus subjects with estimated dietary DII below the median value (N = 331). The NPX of 61 proteins was increased in the plasma of subjects with DII > median vs subjects with DII < median. By contrast, in the latter group, we underscored only 3 proteins with increased NPX. Only 23, out of these 64 proteins, accurately identified subjects with DII > median (Area Under the Curve = 0.601 (0.519–0.668), p = 0.035).
Conclusion:
This large-scale proteomic study supports that higher DII reflects changes in the plasmatic abundance of inflammatory proteins. Larger studies are warranted to validate.
Longitudinal and temporal variation in fish assemblage structure along lowland prairie Neotropical streams and their relationship with environmental variables
Authors: Puerto Juan Martín Paredes del , Colautti Darío Cesar , García Ignacio Daniel , Fanelli Silvia Laura , Paracampo Ariel Hernán 
Published: June 2023

Abstract
The structure of fish assemblages in lotic systems usually registers variations from the headwaters to the mouth related to geomorphological and physicochemical changes. We evaluated the main processes that structure the fish assemblages on the spatial, seasonal, and decadal (2009–2010; 2019–2020) scales and their relationship with environmental variables in two low-impacted Neotropical Pampean streams tributaries to the Río de la Plata river (RdlP). Environmental variables related to the longitudinal variation in the fish assemblage were depth, macrophyte coverage, and altitude, showing higher values in the upper stream sectors compared to the lower ones. Specific richness increased towards lower stream sectors due to the migration of riverine species from the RdlP. The beta diversity partitioning analysis registered that the greatest proportion of variability was explained by the turnover component in both the longitudinal and seasonal analyses. The upper sectors showed a more stable composition, consisting of species of the order Characiformes associated with aquatic macrophytes, while the lower sectors recorded greater instability due to the entry of migrant Siluriformes from the RdlP, highlighting the essential ecological function of Pampean streams for RldP fish assemblages. The decadal comparison showed a sampling site arrangement consistent with the longitudinal position and poorly related to the study period. Therefore, the consistent pattern of longitudinal variation in fish assemblages and its explicative variables have applicability in the conservation and biomonitoring of lowland streams, as well as a reference for recovery efforts.
Comparison of an Ultrasound guided Dynamic Needle Tip Positioning Technique and a Long axis In plane Technique for Radial Artery Cannulation in Elderly Patients: A Prospective Randomized Controlled Study
Authors: Sung Jeong Min , Jun Young Eun , Jung Yun Do , Kim Kyu Nam 
Published: June 2023

Abstract
Purpose
Changes in thickening or hardening of blood vessel walls due to aging can hinder arterial catheter placement in elderly patients. We compared the dynamic needle tip positioning (DNTP) technique and the long-axis in-plane (LAIP) technique in elderly patients.
Methods
This study was conducted on elderly patients aged 75 years or older who underwent general anesthesia requiring placement of an arterial catheter. A total of 160 patients was randomly allocated to the DNTP group or LAIP group. The primary outcome was success rate of first attempt. Additionally, overall success rate, cannulation time, numbers of total redirections and attempts, and occurrence of complications were investigated.
Results
In the DNTP group, the success rate of the first attempt (85% vs. 48.8%, P < 0.001, relative risk = 1.74, 95% CI = 1.37–2.22) and the overall success rate (97.5% vs. 86.3%, P = 0.01, relative risk = 1.13, 95% CI = 1.03–1.24) were significantly higher than in the LAIP group. Fewer redirections (P < 0.001) and attempts (P < 0.001) and a decreased cannulation time (58.8 ± 22.4 seconds vs. 89.6 ± 37.9 seconds, P < 0.001) were detected in the DNTP group. A decreased occurrence of hematoma in the DNTP group was also detected (7.5% vs. 18.8%, P = 0.035, relative risk = 0.40, 95% CI = 0.16–0.98).
Conclusions
The DNTP technique has better efficiency and safety compared with the LAIP technique for radial artery catheterization in the elderly.
The Effect of Perceived Ease of Use and Perceived Enjoyment on Customer Trust and Loyalty in Online Food Delivery Service
Authors: Kurniawan Shelvy , Tankoma Alodia 
Published: June 2023

Competition in the online food delivery industry is getting tougher. Hence, companies in the industry must increase customer trust and loyalty to continue to compete. Factors expected to increase trust and loyalty are perceived ease of use and enjoyment. The research aimed to determine whether there is an influence between perceived ease of use and enjoyment on customer loyalty in the online food delivery service industry in Indonesia, with trust as a mediating variable. The research applied a quantitative method, using SEM with WarpPLS 7.0 software. In addition, the unit of analysis was the users of online food delivery services in various cities in Indonesia with purposive sampling. Using online questionnaires, the research obtained 215 participants. Then, data analysis was done by testing the inner and outer models. The analysis results show an influence between perceived ease of use and perceived enjoyment on customer loyalty, with trust as an intervention variable. All the relationships are found to be positive. Perceived ease of use and perceived enjoyment have a positive and significant effect on the trust of online food delivery service users in Indonesia. Similarly, perceived ease of use and perceived enjoyment affect customer loyalty of online food delivery service users in Indonesia positively and significantly. Last, trust positively and significantly influences customer loyalty of online food delivery service users in Indonesia.
Functioning of community sanitary complexes in rural India: Insights into selected states
Authors: Joe William , Alambusha Ruby , Vijayan Bevin , Sabarisakthi M. , Surwade Sandip , Kumar Abhishek , Mojumdar Sujoy , Banerjee Koushiki 
Published: June 2023

Abstract
An important component of the Swachh Bharat Mission is the construction of community sanitary complexes (CSCs) to address the unmet need for sanitation for households. The success, however, critically hinges on the operational management of CSCs. We examine the functioning status of CSCs in rural areas of five Indian states. Furthermore, we study the association between alternative management arrangements with the functioning and maintenance status of the CSCs. A principal component analysis-based CSC functioning index is created based on the maintenance indicators and sanitary items to identify better-performing CSCs. The mixed-effects logistic regression analysis was applied to examine the association between management arrangement for maintenance and CSC functioning index. Four in every five CSCs were directly managed by the Gram Panchayat. Over 90% of the CSCs have running water supply but electricity connection and lighting arrangement were available in 66.1 and 55.1% CSCs, respectively. The econometric analysis finds that CSCs managed by worship area groups or those managed by village CSC user-groups have better CSC maintenance status. The cleaning frequency of the CSC has a significant association with CSC functioning status. The operational management of CSCs is an area for further policy attention for sustained community sanitation efforts.