bluuebunny/crossref_metadata_2025_split huggingface dataset:
TACC3 is an independent prognostic marker, and knockdown of TACC3 enhances the efficacy of CDK1 inhibitor RO3306 in liver cancer cells
Authors: Guo Enshuang , Yang Jiankun , Li Lei , Chen Jia , Liu Hongqiang 
Published: June 2023

AbstractThe drug resistance of single‐target therapy has gradually become an intractable clinical problem. Combination therapy may be an effective treatment to overcome or postpone drug resistance in cancer. Herein, we discussed the synergistic effect of transforming acidic coiled‐coil containing protein 3 (TACC3) suppression and cyclin‐dependent kinase 1 (CDK1) in hepatocellular carcinoma (HCC). The Cancer Genome Atlas database and bioinformatics methods were implemented to analyze the expression of CDK1 and TACC3, and predict the biological function of TACC3‐related genes in HCC. In addition, in vitro experiments, including cell counting kit 8, transwell and flow cytometry were utilized to evaluate cell proliferation, migration, invasion, cell cycle arrest and apoptosis of HCC cells. Our results demonstrated that TACC3 is an unfavorable and independent prognostic factor to predict poor overall survival (OS) in HCC patients. Genetic inhibition of TACC3 exhibited a remarkable antineoplastic activity of HCC cell lines. Bioinformatic prediction proposed that CDK1 may be the main regulator of TACC3‐related genes in HCC. In vitro experimental measurements suggested that a combination of si‐TACC3 and CDK1 inhibitor synergistically inhibited cell proliferation and migration, and induced G2 cell cycle arrest and apoptosis of HepG2 or MHCC97H cells. In conclusion, our results revealed a prospective dual‐target, TACC3 and CDK1, therapeutic strategy to improve the treatment of HCC.
Blood Pressure Control Following Angiotensin‐Converting Enzyme Inhibitors and Angiotensin II Receptor Blockers: Insights from a Triple‐Blind, Randomized, Clinical Trial
Authors: Sharifan Amin , Bahreini Maryam , Ashraf Haleh , Najmeddin Farhad 
Published: June 2023

AbstractData on substituting one antihypertensive medication with the proper dose of another antihypertensive medication, in certain medical conditions, are scarce. Herein, we present the results of replacing angiotensin‐converting enzyme inhibitors (ACEIs) or angiotensin receptor blockers (ARBs) with the calcium channel blocker (CCB) amlodipine, with or without the alpha‐ and beta‐blocker carvedilol, to control high blood pressure in patients with coronavirus disease 2019 (COVID‐19). Iranian hypertensive patients with COVID‐19 and a history of taking ACEI or ARB were randomized to “continue” and “change” groups. The continue group comprised patients who continued using their previous antihypertensive medication regimen as normal, whereas patients in the change group had their antihypertensive drugs changed to the CCB amlodipine, with or without the alpha‐ and beta‐blocker carvedilol, based on their response to amlodipine. Patients’ blood pressures were measured for 8 days following their recruitment. A total of 31 and 33 patients were randomly allocated to the ACEI/ARB continue and ACEI/ARB change groups, respectively. No significant deviations were seen in patients’ systolic blood pressure by substituting an ACEI/ARB agent with the CCB amlodipine, with or without the alpha‐ and beta‐blocker carvedilol. Moreover, the change group had a more balanced systolic blood pressure (ie, 110–130 mmHg) compared with the continue group (ie, 111.5–140.0 mmHg) throughout their hospitalization period. During their hospitalization, the blood pressure of the change group was well controlled with the proposed equivalent doses. Further investigations of the proposed equivalent doses in larger randomized clinical trials, populations other than Iranian COVID‐19 patients, and extended duration are encouraged (clinical trial registration ID: IRCT20151113025025N3).
Role of the Cu dopant in the textural and catalytic features of the Co/ZnO catalyst for dimethyl oxalate selective hydrogenation
Authors: Kong Xiangpeng , Wu Ruihan , Yuan Peihong , Wu Yuehuan , Wang Ruihong , Chang Xiliang , Wang Man , Wang Xiaoyu , Chen Jiangang 
Published: June 2023

AbstractBACKGROUNDConverting the syngas‐derived dimethyl oxalate (DMO) intermediate to versatile simple diols (ethylene glycol, EG) has been deemed as a promising chemical synthesis technology, owing to the cheap, widely available raw materials. Nonetheless, finding the optimal catalyst for this route is still challenging. In this work, copper (Cu)‐doped cobalt/zinc oxide (Co/ZnO)‐based CoCu/ZnO catalysts for DMO selective hydrogenation to EG were successfully synthesized by the oxalic co‐precipitation method.RESULTSThe Cu content shows significant influence on Co dispersion and chemical features, further determining the resultant structural and morphology properties of the as‐prepared samples. For DMO gas‐phase hydrogenation, the CoCu20/ZnO catalyst prepared in presence of the 20.0 wt% Cu additive exhibited 100.0% DMO conversion and 93.0% EG yield, superior to that of the other catalysts due to adequate active Co0 sites released adjacent to the Cu species. Moreover, the CoCu/ZnO catalyst showed outstanding thermal stability in DMO‐selective hydrogenation for >200 h.CONCLUSIONSThe H2 activation ability and capability of the surface Co species depends critically on the Cu content, further regulating the reaction pathways of the DMO hydrogenation. © 2023 Society of Chemical Industry (SCI).
Treatment of maple syrup urine disease: Benefits, risks, and challenges of liver transplantation
Authors: Deon Marion , Guerreiro Gilian , Girardi Julia , Ribas Graziela , Vargas Carmen Regla 
Published: June 2023

AbstractMaple syrup urine disease (MSUD) is caused by a deficiency in the activity of the branched‐chain α‐ketoacid dehydrogenase (BCKD) complex, promoting the accumulation of the branched‐chain amino acids (BCAA) leucine, isoleucine, and valine, as well as their respective α‐keto acids. MSUD is an autosomal recessive hereditary metabolic disorder characterized by ketoacidosis, ataxia, coma, and mental and psychomotor retardation. The mechanisms involved in the brain damage caused by MSUD are not fully understood. Early diagnosis and treatment, as well as proper control of metabolic decompensation crises, are crucial for patients' survival and for a better prognosis. The recommended treatment consists of a high‐calorie diet with restricted protein intake and specific formulas containing essential amino acids, except those accumulated in MSUD. This treatment will be maintained throughout life, being adjusted according to the patients' nutritional needs and BCAA concentration. Because dietary treatment may not be sufficient to prevent neurological damage in MSUD patients, other therapeutic strategies have been studied, including liver transplantation. With transplantation, it is possible to obtain an increase of about 10% of the normal BCKD in the body, an amount sufficient to maintain amino acid homeostasis and reduce metabolic decompensation crises. However, the experience related to this practice is very limited when considering the shortage of liver for transplantation and the risks related to the surgical procedure and immunosuppression. Thus, the purpose of this review is to survey the benefits, risks, and challenges of liver transplantation in the treatment of MSUD.
Between‐hospital variation in clinical decision support availability for common inpatient pediatric conditions: Results of a national Pediatric Research in Inpatient Settings (PRĪS) Network survey
Authors: Carr Leah H. , Oluwalade Bolu , Muthu Naveen , Beus Jonathan , Bonafide Christopher P. 
Published: June 2023

AbstractImplementing pediatric‐focused clinical decision support (CDS) into hospital electronic health records can lead to improvements in patient care and accelerate quality improvement and research initiatives. However, its design, development, and implementation can be a time‐consuming and costly endeavor that may not be feasible for all hospital settings. In this cross‐sectional study, we surveyed Pediatric Research in Inpatient Settings (PRĪS) Network hospitals about the availability of CDS tools to gain an understanding of the functionality available across 8 common inpatient pediatric diagnoses. Among the conditions, asthma had the most extensive CDS availability, while mood disorders had the least. Overall, freestanding children's hospitals had the greatest breadth in CDS coverage across conditions and depth in CDS types within conditions. Future initiatives should examine the relationship between CDS availability and clinical outcomes as well as its relationship with hospitals’ performance executing multicenter informatics projects, quality improvement collaboratives, and implementation science strategies.
Teaching and researching International Development: Amplifying voices from the Global South
Authors: Zimbalist Zack , Ramírez Jorge Antonio Asprón 
Published: June 2023

AbstractAcross academic disciplines, the knowledge that is produced and taught about International Development overwhelmingly comes from writers and academics who are from (and based in) the Global North. This phenomenon has major consequences for students, scholars, development practitioners and donors across the world. Drawing on data from university course syllabi and a small sample of faculty interviews, this article sheds further light on this reality and delineates some of the most significant challenges. Finally, we highlight efforts to address this problem and how others can contribute to improving our understanding of historically contingent and complex development processes across diverse contexts.
Additional occult cancers identified on staging breast MRI: imaging appearances and pathologic characteristics
Authors: Nassar Lara , Nakad Sanaa , Abou Zeid Farah , Farah Zeina , Saheb Ghida , Mroueh Nayla , Debs Patrick , Berjawi Ghina 
Published: June 2023

AbstractIntroductionBreast magnetic resonance imaging (MRI) is increasingly being used for staging of patients with breast cancer due to its high sensitivity in detecting additional cancers (ACs). However, the clinical impact of diagnosing and treating these cancers remains unclear.MethodsA retrospective study was undertaken of patients with newly diagnosed breast cancer who underwent staging MRI at The American University of Beirut Medical Centre (AUBMC) between 2012 and 2020. Pathology reports and breast MRI examinations were reviewed. Eighteen breast cancer patients with 19 pathology‐proven index cancers (ICs) and 19 pathology‐proven MRI‐detected ACs were included. Chi‐square and Fisher's exact tests for categorical variables and Wilcoxon signed rank test for numerical variables were used to compare ICs to ACs.ResultsThe ICs consisted of four ductal carcinoma in situ (DCIS), 13 invasive ductal carcinomas (IDC), of which five with associated DCIS, and two invasive lobular carcinomas, (ILC) of which one with associated DCIS. ACs comprised 12 DCIS, five IDC, two with associated DCIS and two ILC, one with associated DCIS. Interval cancers were more frequently invasive whereas ACs were more frequently in situ (P = 0.021). ACs were more frequently nuclear grade 2 (P = 0.009). There was no statistically significant difference between ICs and ACs in lesion type (P = 0.062), shape (P = 0.073), initial enhancement (P = 1), delayed enhancement (P = 0.732), hormonal receptor profile (P = 0.68) and Ki67 (P = 0.388). Among ACs, ten (53%) were larger than 10 mm of which five (26%) were invasive cancers, and five (26%) were larger than the ICs.ConclusionsACs detected by breast MRI were more likely to be in situ and to show a nuclear grade 2. Although not reaching statistical significance, some ACs tend to be clinically significant by their type, size or nuclear grade. The impact on clinical management remains to be determined.
COVID‐19db linkage maps of cell surface proteins and transcription factors in immune cells
Authors: Ramjattun Koushul , Ma Xiaojun , Gao Shou‐Jiang , Singh Harinder , Osmanbeyoglu Hatice Ulku 
Published: June 2023

AbstractThe highly contagious SARS‐CoV‐2 and its associated disease (COVID‐19) are a threat to global public health and economies. To develop effective treatments for COVID‐19, we must understand the host cell types, cell states and regulators associated with infection and pathogenesis such as dysregulated transcription factors (TFs) and surface proteins, including signaling receptors. To link cell surface proteins with TFs, we recently developed SPaRTAN (Single‐cell Proteomic and RNA‐based Transcription factor Activity Network) by integrating parallel single‐cell proteomic and transcriptomic data based on Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE‐seq) and gene cis‐regulatory information. We apply SPaRTAN to CITE‐seq data sets from patients with varying degrees of COVID‐19 severity and healthy controls to identify the associations between surface proteins and TFs in host immune cells. Here, we present COVID‐19db of Immune Cell States (https://covid19db.streamlit.app/), a web server containing cell surface protein expression, SPaRTAN‐inferred TF activities, and their associations with major host immune cell types. The data include four high‐quality COVID‐19 CITE‐seq data sets with a toolset for user‐friendly data analysis and visualization. We provide interactive surface protein and TF visualizations across major immune cell types for each data set, allowing comparison between various patient severity groups for the discovery of potential therapeutic targets and diagnostic biomarkers.
Effects of low temperature and highlight stress on lipid accumulation and cell structure of Tropidoneis maxima
Authors: Luo Yuanyuan , Ding Yuhui , Jiang Xiamin , Zeng Guoquan , Peng Ruibing , Han Qingxi , Jiang Maowang 
Published: June 2023

AbstractTropidoneis maxima is a marine diatom with a rapid growth rate that produces high levels of lipids. To explore whether the lipid content could be further enhanced, cultures were first incubated under optimal conditions and then stressed under low temperature (10°C), a high light intensity level (80 μmol/m2·s), and the two factors together (interaction treatment). The results indicated that high light intensity and the temperature‐light interaction exhibited greater impacts on lipid synthesis of T. maxima than low temperature. The two stress treatments increased lipid content by 17.16% and 16.6% compared to the control. In particular, higher biomass concentration was obtained with high light intensity (1.082 g L−1) and low temperature (1.026 g L−1). Moreover, high light intensity (9.06%) and interaction (10.3%) treatments yielded lower starch content compared to low temperature (14.27%) at the end of the stress culture. After 3 days of stress culture, the high light intensity treatment resulted in a 97.01% increase in cell wall thickness and an 18.46% decrease in cell diameter. The results suggest that high light intensity stress on T. maxima would open a new approach to cost‐effective biolipid production.
Multi‐target bioactive compound screening from the infructescence of Platycarya strobilacea Sieb. et Zucc. by affinity chromatography using immobilized β2‐adrenoceptor and muscarinic‐3 acetylcholine receptor as the stationary phase
Authors: Zhang HaoSen , Wang Jing , Wang Chaozhan 
Published: June 2023

As a main source for the recognition and identification of lead compounds, traditional Chinese medicine plays a pivotal role in preventing diseases for years. However, screening bioactive compounds from traditional Chinese medicine remains challenging because of the complexity of the systems and the occurrence of the synergic effect of the compounds. The infructescence of Platycarya strobilacea Sieb. et Zucc is prescribed for allergic rhinitis treatment with unknown bioactive compounds and unclear mechanisms. Herein, we immobilized the β2‐adrenoceptor and muscarine‐3 acetylcholine receptor onto the silica gel surface to prepare the stationary phase in a covalent bond through one step. The feasibility of the columns was investigated by the chromatographic method. Ellagic acid and catechin were identified as the bioactive compounds targeting the receptors. The binding constants of ellagic acid were calculated to be (1.56 ± 0.23)×107 M−1 for muscarine‐3 acetylcholine receptor and (2.93 ± 0.15)×107 M−1 for β2‐adrenoceptor by frontal analysis. While catechin can bind with muscarine‐3 acetylcholine receptor with an affinity of (3.21 ± 0.05)×105 M−1. Hydrogen bonds and van der Waals’ force were the main driving forces for the two compounds with the receptors. The established method provides an alternative for multi‐target bioactive compound screening in complex matrices.
Nomogram Analysis Based on Clinical and Sonographic Characteristics for the Assessment of Postpartum Stress Urinary Incontinence
Authors: Xiao Ting , Cao Yunqing , Zhen Chaojiong , Chen Ziman , Huang Weijun , Su Zhongzhen 
Published: June 2023

ObjectivesWe aimed to develop and validate a nomogram integrating clinical and sonographic characteristics for the individualized SUI risk evaluation in the early postpartum stage.MethodsThis was a prospective cross‐sectional study. From June 2020 to September 2022, singleton primiparas who underwent TPUS examination at 6–8 weeks postpartum were recruited. They were divided into the training and validation cohorts at a ratio of 8:2 according to the temporal split. All subjects were interviewed before TPUS examination. Univariate and multivariate logistic analyses were performed to develop three models: the clinical, sonographic, and combined models. The ROC curve was plotted to evaluate model discrimination ability. Finally, the combined model was selected to establish the nomogram. The nomogram's discrimination, calibration, and clinical usefulness were evaluated in the training and validation cohorts.ResultsThe performance of the combined model was better than that of the clinical and sonographic models. Six predictors (BMI, delivery mode, lateral episiotomy, SUI during pregnancy, cystocele, and bladder neck funneling) remained in the combined model. The nomogram based on the combined model had good discrimination with AUCs of 0.848 (95% CI: 0.796–0.900) and 0.872 (95% CI: 0.789–0.955) in the training and validation cohorts, respectively, and the calibration curve showed good efficiency in assessing postpartum SUI. Decision curve analysis showed that the nomogram was clinically useful.ConclusionsThe nomogram based on clinical and sonographic characteristics showed good efficiency in assessing postpartum SUI risk and can be a convenient and reliable tool for individual SUI risk assessment.
Advancing wildlife connectivity in land use planning: a case study with four‐toed salamanders
Authors: Wade Bryce S. , Carter Evin T. , Derolph Christopher R. , Byrd Greg , Darling Sarah E. , Hayter Lindsey E. , Jett R. Trent , Herold Jamie M. , Giffen Neil R. 
Published: June 2023

AbstractStable habitat connections that wildlife can safely traverse are essential to biodiversity conservation and healthy ecosystems. We developed high‐resolution landscape connectivity models to predict resistance to movement by a threatened wetland‐obligate amphibian, the four‐toed salamander (Hemidactylium scutatum), and identified priority management areas on the 13,000‐ha Department of Energy Oak Ridge Reservation (ORR) from 2019 to 2022. We developed a resistance surface based on aerial light detection and ranging data (LiDAR), >30 years of field‐based mapping of forest, hydrologic, and geologic features, and contemporary population surveys, alongside derived predictors at <1‐m resolution. We then modeled predicted movement corridors using a circuit theory‐based modeling approach. We worked closely with land management and natural resources personnel to integrate ecological modeling with broader land use priorities, monetary costs, and feasibility. We identified important terrestrial and aquatic areas on ORR and simulated management scenarios to promote stable connections for four‐toed salamanders. This approach allowed us to narrow down a list of 438 potential habitat manipulation sites to 10 sites where open‐bottomed culverts and buffers could be implemented. This smaller‐scale restoration approach produced a similar increase in landscape connectivity while costing <20% of a larger‐scale approach based on barrier removal. We successfully identified feasible, cost‐effective management strategies that integrated knowledge from a variety of sources. We offer a strategy that permitted integration of wildlife management goals into infrastructure upgrades wherein wildlife was not an initial consideration.
Endoscopic Endonasal Approach for Trigeminal Schwannomas: Tailored Approaches Based on Lesion Traits
Authors: Wu Xiao , Pan Lai Sheng , Wu Bo Wen , Wu Jie , Chen Yu Xin , Xie Shen Hao , Wan Xi Chen , Din Han , Zhan Jie , Xiao Li Min , Tang Bin , Hong Tao 
Published: June 2023

ObjectivesTo describe four endoscopic endonasal subapproaches, namely, the trans‐lamina papyracea, trans‐prelacrimal recess, trans‐Meckel's cave, and transclival approaches for trigeminal schwannomas (TSs).MethodsThis retrospective study reviewed the medical records and intraoperative videos of 38 patients with TSs who underwent endoscopic endonasal approach (EEA) between Jan 2013 and Dec 2021.ResultsAccording to Jeong's classification, for TS equally in middle and posterior fossae (MP), a purely trans‐Meckel's cave approach was carried out in 2 cases, and a combined transclival approach was carried out in 4 cases. The four tumors that involved infratemporal fossa (two E3, one mE3, and one Mpe3) were performed via a trans‐prelacrimal recess approach, and type Mpe3 was also assisted by the trans‐Meckel's cave approach. One patient with type E1 was treated with a trans‐lamina papyracea approach. The other 27 cases, including type M, Mp, ME2, and MpE2, were all removed by a purely trans‐Meckel's cave approach. Thirty‐six patients (97.4%) received total resection under a purely EEA. The functional abilities and preoperative symptoms of 31 patients (88.6%) improved. Eight (21.1%) patients experienced permanent neurological function deficits. Postoperative cerebrospinal fluid and intraoperative internal carotid artery injury occurred in 1 (2.6%) patient.ConclusionAccording to the specific endoscopic endonasal subapproaches corresponding to the different TS locations, satisfactory results can be obtained for most types of tumors. It represents an effective alternative to the open transcranial approach and can also be properly used in most types of TS with experienced hands.Level of Evidence4 Laryngoscope, 133:2564–2571, 2023