bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective evaluation of Gadoxetate-enhanced magnetic resonance imaging and computed tomography for hepatocellular carcinoma detection and transplant eligibility assessment with explant histopathology correlation
Authors: Jhaveri Kartik S. , Babaei Jandaghi Ali , Bhayana Rajesh , Elbanna Khaled Y. , Espin-Garcia Osvaldo , Fischer Sandra E. , Ghanekar Anand , Sapisochin Gonzalo 
Published: February 2023

Abstract
Background
We aimed to prospectively compare the diagnostic performance of gadoxetic acid-enhanced MRI (EOB-MRI) and contrast-enhanced Computed Tomography (CECT) for hepatocellular carcinoma (HCC) detection and liver transplant (LT) eligibility assessment in cirrhotic patients with explant histopathology correlation.

Methods
In this prospective, single-institution ethics-approved study, 101 cirrhotic patients were enrolled consecutively from the pre-LT clinic with written informed consent. Patients underwent CECT and EOB-MRI alternately every 3 months until LT or study exclusion. Two blinded radiologists independently scored hepatic lesions on CECT and EOB-MRI utilizing the liver imaging reporting and data system (LI-RADS) version 2018. Liver explant histopathology was the reference standard. Pre-LT eligibility accuracies with EOB-MRI and CECT as per Milan criteria (MC) were assessed in reference to post-LT explant histopathology. Lesion-level and patient-level statistical analyses were performed.

Results
Sixty patients (49 men; age 33–72 years) underwent LT successfully. One hundred four non-treated HCC and 42 viable HCC in previously treated HCC were identified at explant histopathology. For LR-4/5 category lesions, EOB-MRI had a higher pooled sensitivity (86.7% versus 75.3%, p <  0.001) but lower specificity (84.6% versus 100%, p <  0.001) compared to CECT. EOB-MRI had a sensitivity twice that of CECT (65.9% versus 32.2%, p <  0.001) when all HCC identified at explant histopathology were included in the analysis instead of imaging visible lesions only. Disregarding the hepatobiliary phase resulted in a significant drop in EOB-MRI performance (86.7 to 72.8%, p <  0.001). EOB-MRI had significantly lower pooled sensitivity and specificity versus CECT in the LR5 category with lesion size < 2 cm (50% versus 79%, p = 0.002 and 88.9% versus 100%, p = 0.002). EOB-MRI had higher sensitivity (84.8% versus 75%, p <  0.037) compared to CECT for detecting < 2 cm viable HCC in treated lesions. Accuracies of LT eligibility assessment were comparable between EOB-MRI (90–91.7%, p = 0.156) and CECT (90–95%, p = 0.158).

Conclusion
EOB-MRI had superior sensitivity for HCC detection; however, with lower specificity compared to CECT in LR4/5 category lesions while it was inferior to CECT in the LR5 category under 2 cm. The accuracy for LT eligibility assessment based on MC was not significantly different between EOB-MRI and CECT.

Trial registration
ClinicalTrials.gov Identifier: NCT03342677, Registered: November 17, 2017.
Agreement between a new optical low coherence reflectometry biometer and an anterior segment optical coherence tomographer
Authors: Wang Yiran , Wan Ting , Liu Luze , Xue Yuyuan , Chen Xinyao , Savini Giacomo , Schiano-Lomoriello Domenico , Zhou Xingtao , Yu Jinjin , Huang Jinhai 
Published: February 2023

Abstract
Background
To assess agreement of measurements between a new optical low coherence reflectometry (OLCR) biometer (SW-9000, Suoer, Tianjin, China) and a spectral-domain optical coherence tomographer (SD-OCT)/Placido topographer (MS-39, CSO, Florence, Italy) in healthy subjects.

Methods
A total of 66 right eyes from 66 healthy subjects were enrolled in this prospective study. Three consecutive measurements were randomly obtained with both devices by the same experienced operator to assess agreement. Bland-Altman plots and 95% limits of agreement (LoA) were used to verify the agreement between the devices. Results are presented as mean ± standard deviation (SD).

Results
The SD-OCT/Placido tomographer showed high agreement with the OLCR biometer for all parameters included in this study. The mean differences of central corneal thickness (CCT), anterior chamber depth (ACD), aqueous depth (AQD), mean keratometry (Km) and corneal diameter (CD) were 2.21 ± 2.67 μm (P < 0.001), − 0.10 ± 0.03 mm (P < 0.001), − 0.10 ± 0.04 mm (P < 0.001), − 0.01 ± 0.22 D (P = 0.773) and 0.20 ± 0.16 mm (P < 0.001), respectively. This implies that the inter-device difference in Km was not statistically significant, while the differences in CCT, ACD, AQD, CD were statistically but not clinically significant. The 95% LoAs of CCT, ACD, AQD, Km and CD were − 3.01 to 7.44 μm, − 0.16 to − 0.05 mm, − 0.18 to − 0.03 mm, − 0.45 to 0.43 D, and − 0.12 to 0.51 mm, respectively.

Conclusions
For CCT, ACD, AQD, Km, and CD in healthy subjects, the new OLCR biometer has high agreement with the SD-OCT/Placido tomographer and can be used interchangeably due to the narrow range of 95% LoAs.
Innate immune responses in pneumonia
Authors: Korkmaz Filiz T. , Traber Katrina E. 
Published: February 2023

AbstractThe lungs are an immunologically unique environment; they are exposed to innumerable pathogens and particulate matter daily. Appropriate clearance of pathogens and response to pollutants is required to prevent overwhelming infection, while preventing tissue damage and maintaining efficient gas exchange. Broadly, the innate immune system is the collection of immediate, intrinsic immune responses to pathogen or tissue injury. In this review, we will examine the innate immune responses of the lung, with a particular focus on their role in pneumonia. We will discuss the anatomic barriers and antimicrobial proteins of the lung, pathogen and injury recognition, and the role of leukocytes (macrophages, neutrophils, and innate lymphocytes) and lung stromal cells in innate immunity. Throughout the review, we will focus on new findings in innate immunity as well as features that are unique to the lung.
Comparative evaluation of ZMYND-8 and RARβ2 genes promoters’ methylation changes in tumor and tumor margin tissues of patients with lung cancer
Authors: Pourasghariazar Mahdieh , Zarredar Habib , Asadi Milad , Caner Ayse , Akhgari Aisan , Valizadeh Hamed , Bornehdeli Soghra , Hashemzadeh Shahryar , Raeisi Mortaza 
Published: February 2023

Abstract
Background
Lung cancer remains one of the most lethal carcinomas worldwide because of its late diagnosis. One of the DNA modifications is methylation, one of the primary alterations of tumor development, consisting of fascinating indicators for cancer diagnosis. This study investigated ZMYND-8 and RARβ2 gene methylation in NSCLC as a new epigenetic tool.

Methods
First, to find out the potential diagnostic capability of ZMYND-8 and RARβ2 genes methylation, we entirely surfed DNA methylation microarrays from the Cancer Genome Atlas (TCGA) data of NSCLC samples. Additionally, we took advantage of using q-MSP in several pieces comprising NSCLC tumors and neighboring normal tissues; ZMYND-8 and RARβ2 genes methylation grades were acquired.

Results
Our finding displayed significant hypomethylation of ZMYND-8 and hypermethylation of RARβ2 in NSCLC samples compared to neighboring standard specimens, which significantly correlated with the clinical stage of malignancy. In addition, the incredible precision of ZMYND-8 and RARβ2 methylations as reliable cancer diagnosis indicators in NSCLC was confirmed, drawing the ROC curve analysis with an AUC value of 0.751 and 0.8676, respectively, for ZMYND-8 and RARβ2. Additional studies of other dominant cancer entities in TCGA displayed that RARβ2’s higher methylation degree and ZMYND-8 lower methylation degree are prevalent changes in tumor evolution which could be possibly considered as a potential diagnostic biomarkers for lung cancer.

Conclusion
Based on this study, ZMYND-8 and RARβ2 methylation are reliable biomarkers for lung cancer.
Laparoscopic assisted removal of large ovarian mass causing precocious puberty in an infant—a case report
Authors: Shah Amar , Lahmar Abdelilah , Momin Elina , Momin Shabbir , Shah Anirudh 
Published: February 2023

Abstract

Background

Sex cord and stromal tumors are a heterogeneous group of tumors that arise from gonadal sex cord cells, gonadal stromal cells, or both. They are divided into pure stromal tumors, pure sex cord tumors, and mixed tumors. Some of these tumors are hormonally active, producing androgens and estrogens, and may therefore exhibit virilization or excess estrogen. Sertoli-Leydig cell tumors are rare tumors belonging to mixed tumors representing less than 0.5% of ovarian tumors. Few cases have been reported in the medical literature.

Case presentation
We report the case of a 1-year-old girl who was admitted for breast lumps and pubic hair with intermittent painless vaginal bleeding in the past 2 weeks. The abdominal examination objectified the presence of an intraperitoneal mass to which an abdominal ultrasound and computerized tomography (CT) scan had confirmed the presence of a right ovarian mass. A laparoscopy was performed which showed an encapsulated mass arriving from the right ovary replacing the normal ovarian tissue. Laparoscopic-assisted removal of the entire mass was done. The postoperative course was uneventful. The histopathological study demonstrated an intermediately differentiated Sertoli cell tumor with an intact capsule, no lymphovascular invasion, or heterologous elements (TNM stage 1).

Conclusions
Although Sertoli-Leydig tumors are commonly seen after the second decade of life in young women, their discovery before puberty is not uncommon. Mass syndrome and signs of virilization are two frequently reported signs that should prompt the clinician to mention this entity among the differential diagnoses. The first-line radiological examination is pelvic ultrasound. The treatment is mainly based on surgery. The prognosis is excellent especially if the tumor is well differentiated.
Efficacy of botulinum toxin in treatment of bruxism and temporomandibular disorders. Systematic review
Authors: Swacha Weronika , Pikulicka Agata , Szulc Izabela , Pacek Katarzyna , Piekarska Małgorzata , Kasperski Radosław , Jedlina Klaudia , Rusin Klaudia , Budziłło Julia , Dobrzańska Justyna 
Published: February 2023

The medical use of botulinum toxin nowadays is widespread. Recent years show that use of botulinum toxin is not only used to control the muscular overactivity in bruxism, tension in temporomandibular disorders but also it can be applied for cosmetic reasons. The objective of this study was to view recent discoveries in this subject by conducting a systemic review of clinical trials published in the research works.
Recent studies present efficacy in releasing pain in both: bruxism and temporomandibular disorders by minimizing symptoms and reducing the intensity of muscle contractions.The scientific works cited confirm a noticeable improvement in most symptoms occurring in the states mentioned above.
Domain-general grit and domain-specific grit: conceptual structures, measurement, and associations with the achievement of German as a foreign language
Authors: Li Chengchen , Yang Yuan 
Published: February 2023

Abstract
The study examined the underlying structures, psychometric properties, and predictive validity of the following domain-general and domain-specific grit scales: The (L2) Grit Scales and the Long-term (L2) Grit Scales. Seven hundred German-as-a-FL learners from six secondary schools in China filled in the four grit scales and a scale measuring self-perceived German proficiency. Two hundred and eighty-nine out of them participated in a subsequent German exam and their German teachers rated their German proficiency. The main results are as follows: (1) both domain-general grit and L2 grit represented a unitary first-order construct with two correlated but distinct factors, namely, consistency of interest and perseverance of efforts; (2) only the Grit Scale and the L2 Grit Scale showed sound psychometric properties; (3) both domain-general grit and L2 grit were positively correlated with L2 achievement; (4) domain-general grit completely lost its predictive power on L2 achievement when combined with L2 grit in the same regression models. Our findings resonate with prior calls for prioritizing L2-specific grit research over domain-general grit research in L2 contexts.
A practical upper-bound efficiency model for solar power plants
Authors: González-Mora Eduardo , Poudel Ram , Durán-García María Dolores 
Published: February 2023

Abstract
A generalized model for the maximum work rate extractable from the Sun is developed considering a reversible and an endoreversible system to define a more practical upper-bound efficiency for the conversion of solar radiation into work and power. This model is based on a photo-thermal work extractor in communication with a high-temperature radiation reservoir and a low-temperature heat sink. Following the model, a parametric analysis of the concentration acceptance product (ξ) and thermal conductance is performed to identify the interdependence of variables for the solar exergy. The results are compared with existing models to provide a practical baseline of work and power extractable from concentrated solar power plants (CSP) technologies. Therefore, it is possible to quantify the irreversibilities of an idealized thermodynamic system operating between the Sun and the absorber (via radiative transfer) and the environment (via convective transfer).
Evaluating the underreporting of patient-reported outcomes in carpal tunnel syndrome randomized controlled trials
Authors: Ladd Chase , Jacobsen Samuel M. , Snider Kelsey , Bacani Rigel , Hillman Cody , Shepard Samuel , Heigle Benjamin , Ottwell Ryan , Hartwell Micah , Vassar Matt 
Published: February 2023

Abstract

Context
In recent years, patient-centered healthcare has become a primary concern for researchers and healthcare professionals. When included in randomized controlled trials (RCTs), patient-reported outcome (PRO) measures serve a critical role in supplementing efficacy outcomes with a patient perspective.

Objectives
The goals of this study are to evaluate the reporting completeness of PROs within literature concerning carpal tunnel syndrome (CTS) utilizing the Consolidated Standards of Reporting Trials Patient-Reported Outcomes (CONSORT-PRO) extension.

Methods
We searched MEDLINE, Embase, and Cochrane Central Register of Controlled Trials (CENTRAL) for published RCTs relating to CTS with at least one PRO measure from 2006 to 2020. Two investigators screened all RCTs for inclusion utilizing Rayyan (https://rayyan.qcri.org/), a systematic review screening platform. In an independent, masked fashion, investigators then evaluated all RCTs utilizing the CONSORT-PRO adaptation and Cochrane Collaboration Risk of Bias (RoB) 2.0 tool. Bivariate regression analyses were utilized to assess relationships between trial characteristics and completeness of reporting.

Results
Our search returned 374 publications, yet only 31 unique RCTs met the inclusion criteria. The mean overall percent of adherence for CONSORT-PRO was 41%. Our secondary outcome—assessing study characteristics—indicated significantly higher completeness of reporting in the absence of a conflict of interest statement (p<0.05), ‘some concerns’ for bias (p<0.005), and when journals required the use of the CONSORT statement (p<0.005). The RoB assessment determined overall suspicion for bias among included RCTs, with 35% (n=11/31) being labeled as ‘high,’ 58% (n=18/31) as ‘some concerns,’ and 7% (n=2/31) as ‘low.’

Conclusions
Our study indicated that the completeness of CONSORT-PRO reporting was deficient within CTS trials. Because of the importance placed on PROs in clinical practice, we recommend adherence to CONSORT-PRO prior to publication of RCTs to increase the understanding of various interventions on patients’ quality of life (QoL).
UHRF1-induced connexin26 methylation is involved in hearing damage triggered by intermittent hypoxia in neonatal rats
Authors: Zhang Xingang , Zheng Jishan , Xu Huiqing , Ma Zhaoxin 
Published: February 2023

Abstract
Ubiquitin-like with plant homeodomain and ring finger domains 1 (UHRF1) promotes the maintenance of established patterns of DNA methylation in mammalian cells. Extensive methylation of connexin26 (COX26) during hearing impairment has been demonstrated. The present study aims to determine whether UHRF1 can induce the methylation of COX26 in cochlea damaged by intermittent hypoxia (IH). After the establishment of the cochlear injury model through IH treatment or isolation of the cochlea containing Corti’s organ, pathological changes were observed via HE staining. Expressions of COX26 and UHRF1 were detected by quantitative reverse-transcription polymerase chain reaction and Western blot. The effect of COX26 methylation levels was analyzed by methylation-specific PCR (MSP). Phalloidin/immunofluorescence staining was used to observe structural changes. The binding relationship between UHRF1 and COX26 was verified by chromatin immunoprecipitation. IH caused cochlear damage, accompanied by increased methylation of COX26 and expression of UHRF1 in the cochlea of neonatal rats. CoCl2 treatment caused the loss of cochlear hair cells, downregulation and hypermethylation of COX26, abnormal upregulation of UHRF1, and disordered expressions of apoptosis-related proteins. UHRF1 in cochlear hair cells binds to COX26, and its knockdown upregulated COX26 level. Overexpressed COX26 partially alleviated the CoCl2-caused cell damage. UHRF1 induces COX26 methylation and aggravates the cochlear damage caused by IH.
3D Spheroids — a Cellular Model for Studying the Effects of Hypoxia on the Epicardial Microenvironment
Authors: Dergilev K. V. , Tsokolaeva Z. I. , Beloglazova I. B. , Traktuev D. O. , Rasulova M. T. , Parfenova E. V. 
Published: February 2023

Fundamental research in recent years has allowed us to reassess the molecular and cellular mechanisms of cardiac ontogenesis and its repair after damage. The epicardium, the outer, tightly adjoining layer of the cardiac wall formed by epicardial mesothelial cells, collagen and elastic ﬁbers, has gained special relevance as an important participant of reparative processes. Better insight into poorly understood epicardial function is challenged due to anatomical issues and lack of relevant cellular models.The aim of this study was to develop a spheroid 3D model of the epicardial microenvironment and determine responses of spheroids to hypoxia.Materials and methods. Spheroids were harvested in V-shaped culture dishes with a low adhesion coating. Immunofluorescent staining of cryosections, histological methods and real-time PCR were used for characterization of cultured spheroids.Results. We demonstrated that cultivation of cells under low adhesion conditions in V-shaped culture dishes resulted in the formation of spheroids with an average size of 136+21 µm and cell viability rates of over 98%. The cells in the spheroids cultured under normoxic conditions formed tight junctions and were characterized by a low level of proliferation and the ability to synthesize extracellular matrix proteins. Under hypoxia cells in the spheroids showed partial loss of intercellular contacts, acquired a spindle shape, started to express HIF1a, SNAIL, COL1Al and accumulate collagen. All these features demonstrated the activation of mesothelial(endothelial)-mesenchymal transition strongly resembling epicardial cellular responses to ischemia in vivo.Conclusion. An epicardial spheroid cell culture model suitable for study cellular responses to hypoxic environment was developed. This model can be used to clarify mechanisms regulating epicardial microenvironment and test new targeted candidate drugs.
3D EEG and Clinical Evidence of Brain Dying. Preliminary Report
Authors: Drobný M. , Drobná Sániová B. , Učňová S. , Sobolová G. , Koyš R. , Machado C. , Machado Ya. 
Published: February 2023

Determination of brain dying means reversible or irreversible injury to the brain, including the brainstem. Current guidelines rely on clinical examination including the proof of coma, absent brain stem reﬂexes, and apnoea test. Neurophysiological testing using electroencephalography and evoked potentials — somatosensory evoked potentials and brainstem auditory evoked potential could have been helpful in the final diagnostic brain death conclusion, but the diagnostic accuracy of these methods in the last years has revealed controversies. Here, we present data on quantitative EEG signal evaluation (qEEG) by a 3-dimensional brain mapping (3D BM) as developing tool to clarify whether the transverse and anterior posterior coherences such as connectivity indices may demonstrate connection in transversal or anterior posterior dimensions with «wavelet transformation» and if the 3D BM visualization of the of representative EEG signals may improve informative value of EEG signals quantification when evaluating the brain dying.The purpose of our work is to provide an update on the evidence and controversies on the use of EEG for determining brain dying and raise discussion on EEG applications to improve the transplantation program.Results. We analyzed the EEG records of 10 patients admitted for cardiopulmonary resuscitation (CPR) during September, 2017 — August, 2018. Data from one patient, ŽM, 33 years old, after haemorrhagic shock (August 2018) were analyzed in details. Quantitative EEG dynamics by images and clinical course of brain dying were monitored prior and after the amantadine sulfate intravenous administration for brain revival. Data demonstrated the ability of brain to survive; the cause of final brain death was heart failure.Conclusion. Data confirm the hope for survival of the brain in a coma and demonstrate brain capability to keep functionally optimal state as a potential for a good social adaptation.
Microorganismos autóctonos como alternativa para la biofertilización de Glycine max (L.) Merrill
Authors: Ceiro Catasú Wilson Geobel , Gaibor Fernández Ramiro Remigio , Vargas Gálvez Carlos Augusto , Botello Guevara Harri , Bonilla Landaverry Gesly , Sosa Sánchez Oandis 
Published: February 2023

Introducción. Soya (Glycine max (L.) Merrill) posee importancia económica y nutricional por sus niveles proteicos y contenido de aceite vegetal de calidad biológica, necesarios para la alimentación humana y animal. Las alternativas microbianas de biofertilización de este cultivo podrían representar una opción para su producción con bajos insumos en condiciones tropicales. Objetivo. Evaluar el efecto de microorganismos autóctonos (MA) como alternativa de biofertilización de G. max en condiciones de bajos insumos de producción. Materiales y métodos. El experimento se estableció en una finca situada en la localidad El Coco, Yara, Granma, Cuba, sobre un suelo Pardo Mullido Carbonatado, durante el año 2021. Se usaron semillas certificadas de G. max cv. Incasoy-27. La siembra se realizó en primavera a una distancia de 0,70 m x 0,04 m. Se utilizaron tres dosis de MA (12, 24 y 36 L ha-1) y un testigo absoluto. Se evaluaron variables del crecimiento, rendimiento y sus componentes. Se utilizó un diseño de bloques al azar; los datos se procesaron mediante ANDEVA, regresión lineal, correlación y análisis de componentes principales. Resultados. Los MA bioestimularon el crecimiento de G. max en un rango de 12 % y 24 %, mientras que el rendimiento se favoreció entre 16 % y 44 %. El análisis de correlación de Pearson señaló que la masa de semillas por planta y la cantidad de vainas por planta, constituyeron las variables más correlacionadas con el rendimiento. Conclusión. Con la inclusión de MA se logró una bioestimulación del crecimiento en longitud de las plantas de G. max cv. Incasoy-27 en comparación con el testigo y el rendimiento agrícola se potenció.