bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome assembly of the bearded irisIris pallidaLam
Authors: Bruccoleri Robert E. , Oakeley Edward J. , Faust Ann Marie E , Altorfer Marc , Dessus-Babus Sophie , Burckhardt David , Oertli Mevion , Naumann Ulrike , Petersen Frank , Wong Joanne 
Published: September 2023

AbstractIrises are perennial plants, representing a large genus with hundreds of species. While cultivated extensively for their ornamental value, commercial interest in irises lies in the secondary metabolites present in their rhizomes. The Dalmatian Iris (Iris pallidaLam.) is an ornamental plant that also produces secondary metabolites with potential value to the fragrance and pharmaceutical industries. In addition to providing base notes for the fragrance industry, iris tissues and extracts possess anti-oxidant, anti- inflammatory, and immunomodulatory effects. However, study of these secondary metabolites has been hampered by a lack of genomic information, instead requiring difficult extraction and analysis techniques. Here, we report the genome sequence ofIris pallidaLam., generated with Pacific Bioscience long-read sequencing, resulting in a 10.04 Gbp assembly with a scaffold N50 of 14.34 Mbp and 91.8% complete BUSCOs. This reference genome will allow researchers to study the biosynthesis of these secondary metabolites in much greater detail, opening new avenues of investigation for drug discovery and fragrance formulations.Research area: Genetics and Genomics; Botany; Plant Genetics
Self-control is associated with health-relevant disparities in buccal DNA-methylation measures of biological aging in older adults
Authors: Willems Y.E. , deSteiguer A. , Tanksley P.T. , Vinnik L. , Främke D. , Okbay A. , Richter D. , Wagner G. G. , Hertwig R. , Koellinger P. , Tucker-Drob E.M. , Harden K. P. , Raffington L. 
Published: September 2023

AbstractSelf-control is a personality dimension that is associated with better physical health and a longer lifespan. Here we examined (1) whether self-control is associated with buccal and saliva DNA-methylation (DNAm) measures of biological aging quantified in children, adolescents, and adults, and (2) whether biological aging measured in buccal DNAm is associated with self-reported health. Following preregistered analyses, we computed two DNAm measures of advanced biological age (PhenoAge and GrimAge Acceleration) and a DNAm measure of pace of aging (DunedinPACE) in buccal samples from the German Socioeconomic Panel Study (SOEP-G[ene],n= 1058, age range 0-72,Mage= 42.65) and saliva samples from the Texas Twin Project (TTP,n= 1327, age range 8-20,Mage= 13.50). We found that lower self-control was associated with advanced biological age in older adults (β=-.34), but not young adults, adolescents or children. This association was not accounted for by statistical correction for socioeconomic contexts, BMI, or genetic correlates of low self-control. Moreover, a faster pace of aging and advanced biological age measured in buccal DNAm were associated with worse self-reported health (β=.13 toβ= .19). But, effect sizes were weaker than observations in blood, thus customization of DNAm aging measures to buccal and saliva tissues may be necessary. Our findings are consistent with the hypothesis that self-control is associated with health via pathways that accelerate biological aging in older adults.
Symptom experience before vs. after confirmed SARS-CoV-2 infection: a population and case control study using prospectively recorded symptom data
Authors: Sudre Carole H. , Antonelli Michela , Cheetham Nathan J , Molteni Erika , Canas Liane S , Bowyer Vicky , Murray Ben , Rjoob Khaled , Modat Marc , Pujol Joan Capdevila , Hu Christina , Wolf Jonathan , Spector Tim D , Hammers Alexander , Steves Claire J , Ourselin Sebastien , Duncan Emma L 
Published: September 2023

AbstractBackgroundSome individuals experience prolonged illness after acute COVID-19. We assessed whether pre-infection symptoms affected post-COVID illness duration.MethodsSurvival analysis was performed in adults (n=23,452) with community-managed SARC-CoV-2 infection prospectively self-logging data through the ZOE COVID Symptom Study app, at least weekly, from 8 weeks before to 12 weeks after COVID-19 onset, conditioned on presence vs. absence of baseline symptoms (4-8 weeks before COVID-19). A case-control study was performed in 1350 individuals with long illness (≥8 weeks, 906 [67.1%] with illness ≥12 weeks), matched 1:1 (for age, sex, body mass index, testing week, prior infection, vaccination, smoking, index of multiple deprivation) with 1350 individuals with short illness (<4 weeks). Baseline symptoms were compared between the two groups; and against post-COVID symptoms.FindingsIndividuals reporting baseline symptoms had longer post-COVID symptom duration (from 10 to 15 days) with baseline fatigue nearly doubling duration. Two-thirds (910 of 1350 [67.4%]) of individuals with long illness were asymptomatic beforehand. However, 440 (32.6%) had baseline symptoms, vs. 255 (18.9%) of 1350 individuals with short illness (p<0.0001). Baseline symptoms increased the odds ratio for long illness (2.14 [CI: 1.78; 2.57]). Prior comorbidities were more common in individuals with long vs. short illness. In individuals with long illness, baseline symptomatic (vs. asymptomatic) individuals were more likely to be female, younger, and have prior comorbidities; and baseline and post-acute symptoms and symptom burden correlated strongly.InterpretationIndividuals experiencing symptoms before COVID-19 have longer illness duration and increased odds of long illness. However, many individuals with long illness are well before SARS-CoV-2 infection.
Linear ablation for persistent atrial fibrillation: an evidence-based study
Authors: Xu Changhao , Li Kaige , Zhu Xiyao , Wang Xinhua , Ye Ping , Jiang Weifeng , Wu Shaohui , Xu Kai , Li Xiangting , Wang Ying , Zheng Qidong , Wang Yanzhe , Leng Lihua , Zhang Zengtang , Han Bing , Zhang Yu , Qin Mu , Liu Xu 
Published: September 2023

AbstractBackgroundAdditional linear ablation for persistent atrial fibrillation (PerAF) still has limited evidence-based medical proof.ObjectivesWe probed into the mechanisms of intermediate atrial tachycardia (AT) during PerAF termination by catheter ablation and provided evidence for it.Methods136 patients who converted to organized AT after PerAF termination in the Extent-AF study were analyzed. Bi-atrial activation mapping combined with entrainment mapping were performed to identify the mechanisms and critical isthmus of these ATs.ResultsA total of 164 ATs in 136 patients were identified (average 1.2 per patients) and 143 (87%) ATs in 113 patients (average 1.3 per patient) were successfully mapped. The mechanisms of intermediate ATs were macro-reentry in 110 (77%), micro-reentry in 21 (15%), and focal AT in 12 (8%). Among the macro-reentrant ATs, the most common were perimitral ATs (PM-AT) 52 (47%), followed by roof dependent ATs (RF-AT) in 40 (36%) and typical atrial flutter (AFL) in 18 (16%). 98 (72%) patients had successfully ablated intermediate ATs. Among these patients, 88 (90%) required at least one of the perimitral line, roofline, or peritricuspid line to finally restore sinus rhythm. At the end of 12 months of follow-up, 63 (64.3%) patients with successful ablative ATs were free of any arrhythmia.ConclusionThe majority of intermediate ATs after PerAF termination were macro-reentrant ATs. Linear ablation targets the mitral isthmus, roof, and tricuspid isthmus was a critical step of PerAF ablation to restore sinus rhythm in up to 90% patients, suggesting the importance of additional linear ablation.
WITHDRAWN: Caries Lesion Assessment Using 3D Virtual Models By Examiners with Different Degrees of Clinical Experience
Authors: Porumb Ioana , Leucuta Daniel Corneliu , Banut Marius , Culic Bogdan , Lucaciu Ondine Patricia , Culic Carina , Simu Meda-Romana , Badea Iulia Clara , Leghezeu Alexa-Nicole , Sandu Iulia , Nigoghossian Marion 
Published: September 2023

Withdrawal StatementThe authors have withdrawn their manuscripthttps://www.medrxiv.org/content/10.1101/2023.08.30.23294877v1owing to the fact that they have new data and they are currently preparing an updated version.Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author of MS ID#: MEDRXIV/2023/294877
Altered iron-sulfur cluster transfer in Arabidopsis mitochondria reveals lipoyl synthase as a Janus-faced enzyme that generates toxic sulfide
Authors: Pedroletti Luca , Moseler Anna , Timm Stefan , Poschet Gernot , Homagk Maria , The Jeremy X. L. , Wagner Stephan , Wirtz Markus , Hell Rüdiger , Meyer Andreas J. 
Published: September 2023

AbstractIron–sulfur (Fe–S) cluster are vital cofactors in all domains of life. Mitochondrial Fe–S cluster assembly occurs in two major steps to first build [2Fe–2S] clusters and subsequently assemble these into [4Fe–4S] clusters. The two assembly machineries are interconnected by glutaredoxin S15 (GRXS15) that transfers [2Fe–2S] clusters to the second machinery. Diminished cluster transfer activity of GRXS15 in Arabidopsis mitochondria causes specific defects associated with lipoyl synthase (LIP1) activity. Conversely, overexpression ofLIP1in wild-type plants causes the release of toxic amounts of sulfide that can be detoxified by increasing the capacity for sulfide fixation through overexpression ofO-acetylserine-(thiol)-lyase. The release of sulfide by lipoyl synthase causes a disturbance of mitochondrial sulfide homeostasis resulting in distinct and readily observable macroscopic phenotypes. These phenotypes enable a direct readout of consequences resulting from defects in Fe–S cluster assembly or targeted modulation of Fe–S cluster flux in mitochondria.
Heterochromatin re-organization associated with the transcriptional reprogramming under viral infection inArabidopsis
Authors: Annacondia Maria Luz , Juarez-Gonzalez Vasti Thamara , Cheng Jinping , Reig-Valiente Juan Luis , Martinez German 
Published: September 2023

AbstractEpigenetic mechanisms are key regulators of genomic integrity and genic expression. Emerging evidence shows that epigenetic regulation is an important component of the transcriptional reprogramming during stress. Despite this, the overall stress-induced reprogramming of the different epigenetic marks and their targets are unknown. Here, we uncovered multiple epigenetic changes taking place during viral infection inArabidopsis thalianaand their connection with gene expression. We find that cucumber mosaic virus (CMV) infection induces an overall reorganization of the repressive epigenetic marks H3K9me2, H3K27me3, and DNA methylation, which interact between them and are dynamic during infection. Overall, these epigenetic changes are involved in the reprogramming of the transcriptional program to adapt to the biotic stress, and might ensure genome stability through the transcriptional control of transposable elements (TEs). Mechanistically, we demonstrate that the catalytic component of the Polycomb Repressive Complex 2 (PRC2) CURLY LEAF (CLF) mediates the transcriptional repression of genes gaining H3K27me3 during viral infection and that mutants on that component induce resistance against CMV. Altogether, our results provide a complete picture of the epigenetic changes that occur during biotic stress and exemplify the overall dynamism of epigenetic regulation in eukaryotic organisms.
Evolutionarily related host and microbial pathways regulate fat desaturation
Authors: Fox Bennett W. , Helf Maximilian J. , Burkhardt Russell N. , Artyukhin Alexander B. , Curtis Brian J. , Palomino Diana Fajardo , Chaturbedi Amaresh , Tauffenberger Arnaud , Wrobel Chester J.J. , Zhang Ying K. , Lee Siu Sylvia , Schroeder Frank C. 
Published: September 2023

ABSTRACTFatty acid desaturation is central to metazoan lipid metabolism and provides building blocks of membrane lipids and precursors of diverse signaling molecules. Nutritional conditions and associated microbiota regulate desaturase expression1–4, but the underlying mechanisms have remained unclear. Here, we show that endogenous and microbiota-dependent small molecule signals promote lipid desaturation via the nuclear receptor NHR-49/PPARα inC. elegans. Untargeted metabolomics of a β-oxidation mutant,acdh-11, in which expression of the stearoyl-CoA desaturase FAT-7/SCD1 is constitutively increased, revealed accumulation of a β- cyclopropyl fatty acid, becyp#1, that potently activatesfat-7expression via NHR-49. Biosynthesis of becyp#1 is strictly dependent on expression of cyclopropane synthase by associated bacteria, e.g.,E. coli. Screening for structurally related endogenous metabolites revealed a β-methyl fatty acid, bemeth#1, whose activity mimics that of microbiota-dependent becyp#1, but is derived from a methyltransferase,fcmt-1, that is conserved across Nematoda and likely originates from bacterial cyclopropane synthase via ancient horizontal gene transfer. Activation offat-7expression by these structurally similar metabolites is controlled by distinct mechanisms, as microbiota-dependent becyp#1 is metabolized by a dedicated β-oxidation pathway, while the endogenous bemeth#1 is metabolized via α-oxidation. Collectively, we demonstrate that evolutionarily related biosynthetic pathways in metazoan host and associated microbiota converge on NHR-49/PPARα to regulate fat desaturation.
Mitosis exit followed by death in interphase prevents the development of polyploid giant cancer cells
Authors: Vicente Juan Jesus , Khan Kainat , Tillinghast Grant , McFaline-Figueroa José L. , Sancak Yasemin , Stella Nephi 
Published: September 2023

AbstractMicrotubule targeting agents (MTAs) are commonly prescribed to treat cancers and predominantly kill cancer cells in mitosis. Significantly, some MTA-treated cancer cells can escape death in mitosis and exit mitosis, and become malignant polyploid giant cancer cells (PGCC). Considering the low number of malignant cells undergoing mitosis in tumor tissue, killing these cells in interphase may represent a favored antitumor approach. We discovered that ST-401, a mild inhibitor of microtubule assembly, preferentially kills cancer cells in interphase as opposed to mitosis, and avoids the development of PGCC. Single cell RNA sequencing identified mRNA transcripts regulated by ST-401, including mRNAs involved in ribosome and mitochondrial functions. Accordingly, ST-401 induces an integrated stress response and promotes mitochondria fission accompanied by a reduction in energy metabolism. This cell response may underly death in interphase and avoid the development of PGCC.
Upstream regulator of genomic imprinting in rice endosperm is a small RNA-associated chromatin remodeler
Authors: Pal Avik Kumar , Sundar G Vivek Hari , N Amruta , Shivaprasad P.V. 
Published: September 2023

AbstractGenomic imprinting is observed in endosperm, a placenta-like seed tissue, where transposable elements (TEs) and repeat-derived small(s)RNAs mediate epigenetic changes in plants. In imprinting, uniparental gene expression arises due to parent-specific epigenetic marks on one allele but not on the other. The importance of sRNAs and their regulation in endosperm development or in imprinting is poorly understood in crops. Here we show that a previously uncharacterized CLASSY (CLSY)-family chromatin remodeler namedOsCLSY3is essential for rice endosperm development and imprinting, acting as an upstream player in sRNA pathway. Comparative transcriptome and genetic analysis indicated its endosperm-preferred expression and its paternally imprinted nature. These important features were modulated by RNA-directed DNA methylation (RdDM) of tandemly arranged TEs in its promoter. Upon perturbation ofOsCLSY3in transgenic lines we observed defects in endosperm development and loss of around 70% of all sRNAs. Interestingly, well-conserved endosperm-specific sRNAs (siren) that are vital for reproductive fitness in angiosperms were dependent onOsCLSY3. We also observed many imprinted genes and seed development-associated genes under the control of CLSY3-dependent RdDM. These results support an essential role ofOsCLSY3in rice endosperm development and imprinting, and propose similar regulatory strategies involvingCLSY3homologs among other cereals.HighlightsUnlike among dicots, in rice and maize,CLSY3is a maternally expressed imprinted gene majorly expressed in endosperm.Endosperm-preferred expression ofOsCLSY3is regulated by RNA-directed DNA methylation at two tandem transposon elements present in its promoter.OsCLSY3is crucial for endosperm development and grain filling. It regulates expression of key seed development and endosperm-specific imprinted genes through RNA directed DNA methylation.
The impact of corporate philanthropy on the evolution of social media brand community – based on the case of ERKE
Authors: Luo Gongli , Hao Junying , Ma He 
Published: September 2023

PurposeCorporate philanthropy is increasingly a vital decision-making basis for consumers to purchase and establish relationships with enterprises. However, few studies have examined corporate philanthropy from the perspective of community evolution. To address this gap, this study aims to provide a more in-depth and holistic investigation of corporate philanthropy by examining the evolution of social media brand communities caused by corporate philanthropy and the characteristics of consumer interactive behavior.Design/methodology/approachWeb crawlers developed by Python were employed to collect data of ERKE from Sina Weibo (the Chinese equivalent of Twitter). A total of 2,736 posts and 7,774 comments were collected and investigated using social network and sentiment tendency analyses.FindingsThe results showed that the evolution of the social media brand community presented a prominent three-stage characteristic influenced by corporate philanthropy. The findings not only support the benefits of corporate philanthropy but also show the possible disadvantages. Besides, this study further concluded the characteristics of consumer interactive behavior in the social media brand community.Originality/valueThis paper addresses an attractive and practical issue related to the impact of corporate philanthropy. Moreover, this study is one of the first studies to examine the impact of corporate philanthropy in the context of the social media brand community. The findings of this study will provide a valuable reference for community operations and practitioners of brands.
Rural accessibility to digital libraries: requirements and challenges
Authors: Mamabolo Mapheto J. , Durodolu Oluwole Olumide 
Published: September 2023

Purpose
This study aims to determine the requirements and find out the challenges for the use of digital library services for rural areas of Capricorn District Municipality, Limpopo province. The research questions of the study are: What are the requirements for the use of digital library services in rural areas of Capricorn District Municipality? What are the challenges of accessing digital library services in rural areas?

Design/methodology/approach
Data was analysed thematically and deductively in this study, as researcher required to accomplish the purpose of the study through consistent structure (Zalaghi and Khazaei, 2016). In deductive analysis, the researcher starts with a set of categories, which are then used to categorize and organize data (Bertram and Christiansen, 2020). The researcher got familiar with data from the interviewing process and when transcribing data from audio tape. The transcription process was done for the coding purposes. Coding allows the researcher to simplify and focus on specific characteristics of data.

Findings
The study findings advocate for the establishment of digital libraries in rural areas. The library authorities are challenged to adapt digital ways of information provision. Since librarians have been providing digital content in libraries for use on users’ laptops and other gadgets, this implies that the Department of Sport, Arts and Culture has been lagging behind in finding innovative ways to provide information, especially in rural areas. To successfully keep libraries as sources of information, transformative measures have to be taken, and where possible, revisit the policies and keep drifting with the societal changes. The library authorities have to delve into new ways of providing LIS to the communities. Unquestionably, information and communication technologies have penetrated our societies and became a way of life. In addition, there are unlimited benefits which can be derived from digital technologies, especially given the lack of physical libraries in rural areas.

Originality/value
To the best of the authors’ knowledge, the academic research is original and has not been published anywhere before.
Think manager – Think male or female: exploring the content of gendered stereotypes of the managerial role among undergraduate business students in Ireland over a 10-year period
Authors: Berkery Elaine , Ryan Nuala F. 
Published: September 2023

Purpose
Using Schein’s Descriptive Index (SDI), this paper aims to first examine gender role stereotypes and requisite managerial characteristics among Irish business students over a 10-year period. Then, the paper investigates whether there have been changes in gender role stereotypes during this period and subsequently unpack the reasons behind any changes recorded.

Design/methodology/approach
In total, 1,124 students from the same business student population rated men, women and managers in general, using SDI. Data was collected first during the academic year 2008–2009 and again in 2018–2019 to determine stability or change in gender role stereotypes and requisite managerial characteristics. Intraclass correlation coefficients scores were computed to determine the relationship between gender and requisite managerial characteristics and identify differences and similarities between the two samples. To explore the content of gender stereotypes, an examination of the specific descriptive items was conducted by performing a factorial analysis using Duncan’s Multiple Range Test. Finally, the authors adapted the scales developed by Duehr and Bono (2006) to determine whether broad gender stereotypic characteristics with respect to communal and agentic, attributed to men, women and managers, differ by sample.

Findings
The overall findings indicate changes in the extent of gender role stereotyping of the managerial role among the male cohorts studied. The subsequent analysis of the descriptive items identified that the change among the male cohort is due to the levels of agency they perceive women to now possess.

Research limitations/implications
The authors contribute to the literature on both gendered and managerial stereotypes by showing changes in the pro-male stereotype of the managerial role and contribute to the existing debate on a shift towards a more androgynous view of leadership.

Practical implications
These findings help understand the content of gender role stereotypes that recent graduates bring with them to their first job post-graduation. The observed changes in the level of agency ascribed to women by their male counterparts could prove to be an important step forward for women’s advancement to managerial positions.

Originality/value
The findings indicate that both male and female cohorts in Sample 2 perceived men and women in general to possess the same levels of communal and agentic traits as their managerial counterparts.