bluuebunny/crossref_metadata_2025_split huggingface dataset:
TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cells
Authors: Ceglowski J. , Hoffman H.K. , Hoff K.J. , McCurdy B.L. , Moore J.K. , Prekeris R. 
Published: July 2023

AbstractThe primary cilium is a critical sensory organelle that is built of axonemal microtubules ensheathed by a ciliary membrane. In polarized epithelial cells, primary cilia reside on the apical surface and must extend these microtubules directly into the extracellular space and remain a stable structure. However, the factors regulating cross-talk between ciliation and cell polarization, as well as, axonemal microtubule growth and stabilization in polarized epithelia are not fully understood. In this study, we find TTLL12, a previously uncharacterized member of the Tubulin Tyrosine Ligase-Like (TTLL) family, localizes to the base of primary cilia and is required for cilia formation in polarized renal epithelial cells. We also show that TTLL12 directly binds to the α/β-tubulin heterodimerin vitroand regulates microtubule dynamics, stability, and post-translational modifications (PTMs). While all other TTLLs catalyze the addition of glutamate or glycine to microtubule C-terminal tails, TTLL12 uniquely affects tubulin PTMs by promoting both microtubule lysine acetylation and arginine methylation. Together, this work identifies a novel microtubule regulator and provides insight into the requirements for apical extracellular axoneme formation.
Pseudomonas aeruginosaAlgF is a protein-protein interaction mediator required for acetylation of the alginate exopolysaccharide
Authors: Low Kristin E. , Gheorghita Andreea A. , Tammam Stephanie D. , Whitfield Gregory B. , Li Yancheng E. , Riley Laura M. , Weadge Joel T. , Caldwell Shane J. , Chong P. Andrew , Walvoort Marthe T. C. , Kitova Elena N. , Klassen John S. , Codée Jeroen D. C. , Howell P. Lynne 
Published: July 2023

ABSTRACTEnzymatic modifications of bacterial exopolysaccharides enhance immune evasion and persistence during infection. In the Gram-negative opportunistic pathogenPseudomonas aeruginosa, acetylation of alginate reduces opsonic killing by phagocytes and improves reactive oxygen species scavenging. Although it is well-known that alginate acetylation inP. aeruginosarequires AlgI, AlgJ, AlgF, and AlgX, how these proteins coordinate polymer modification at a molecular level remains unclear. Here, we describe the structural characterization of AlgF and its protein interaction network. We characterize direct interactions between AlgF and both AlgJ and AlgXin vitro, and demonstrate an association between AlgF and AlgX, as well as AlgJ and AlgI, inP. aeruginosa. We determine that AlgF does not exhibit acetylesterase activity and is unable to bind to polymannuronatein vitro.Therefore, we propose that AlgF functions to mediate protein-protein interactions between alginate acetylation enzymes, forming the periplasmic AlgJFXK (AlgJ-AlgF-AlgX-AlgK) acetylation and export complex required for robust biofilm formation.
Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a
Authors: Sinan Selma , Appleby Nathan M. , Russell Rick 
Published: July 2023

ABSTRACTCRISPR-Cas12a binds and processes a single pre-crRNA during maturation, providing a simple tool for genome editing applications. Here, we constructed a kinetic and thermodynamic framework for pre-crRNA processing by Cas12ain vitro, and we measured the contributions of distinct regions of the pre-crRNA to this reaction. We find that the pre-crRNA binds rapidly and extraordinarily tightly to Cas12a (Kd= 0.6 pM), such that pre-crRNA binding is fully rate limiting for processing and therefore determines the specificity of Cas12a for different pre-crRNAs. The guide sequence contributes 10-fold to the affinities of both the precursor and mature forms of the crRNA, while deletion of an upstream sequence had no significant effect on affinity of the pre-crRNA. After processing, the mature crRNA remains very tightly bound to Cas12a, with a half-life of ∼1 day and aKdvalue of 60 pM. Addition of a 5’-phosphoryl group, which is normally lost during the processing reaction as the scissile phosphate, tightens binding of the mature crRNA by ∼10-fold by accelerating binding and slowing dissociation. Using a direct competition assay, we found that pre-crRNA binding specificity is robust to other changes in RNA sequence, including tested changes in the guide sequence, addition of a 3’ extension, and secondary structure within the guide region. Together our results provide a quantitative framework for pre-crRNA binding and processing by Cas12a and suggest strategies for optimizing crRNA design in some genome editing applications.
Poison pills adoption, real earnings management and IPO failure
Authors: Trabelsi Samir , Chalwati Amna 
Published: July 2023

PurposeThis paper examines the relationship between poison pills, real earnings management and initial public offering (IPO) failure.Design/methodology/approachThe authors sampled 2,997 IPO firms that went public during 1993-2015.FindingsThe authors find that IPO firms manipulate earnings upward using real earnings management. The authors also find that IPO firms exhibiting a higher level of real earnings management have a higher probability of IPO failure. In addition, the authors find that weak shareholders' governance is positively associated with IPO failure.Practical implicationsThese results suggest that poor governance structures in failed firms open the door to manipulating real activities and increasing operational risk.Originality/valueThe study findings are of most significant interest to potential investors and other stakeholders affiliated with a firm going public, an auditor, an underwriter, the lawyers who consult with the firm and employees or executives who might consider joining that firm.
Effects of online platforms on learner's satisfaction: a serial mediation analysis with instructor presence and student engagement
Authors: Jain Ayushi , Sharma Poonam , Meher Jamini Ranjan 
Published: July 2023

PurposeThis research aims to examine the impact of virtual learning platforms and instructor presence (IP) on learner satisfaction (LS). Further, this study examines the role of learner engagement (LE) in order to improve the LS.Design/methodology/approachThis research uses both primary and secondary data sources to compile the research's findings. The primary source of data includes 610 responses from various higher education institutes in India. The collected data were analysed using the partial least square structural equation modelling (PLS-SEM) technique.FindingsThis research provides evidence that the theoretical model is accurate with the gathered data sample. In the model, online platform (OP) is an independent variable, whereas LS is a dependent variable, and IP and LE are the mediating variables. The outcomes demonstrated that OP has a positive impact on IP and LE. Also, the relationships between IP and LE, IP and LS and LE and LS are significantly positive. The mediation analysis validates the importance of the IP and LE for relationships.Originality/valueThis investigation presents a comprehensive model, which demonstrates the relationship between OP, IP, LE and LS. The study makes a unique reference to several theories in order to boost interaction and IP in virtual learning, the learner's learning experience can be enhanced. The model helps teachers and educational institutions formalise strategies to boost interaction and examine the institutions' pedagogy to enhance satisfaction.
Inclusive business, private sector credit and economic welfare: evidence from Africa
Authors: Ofori-Sasu Daniel , Dzisi Smile , Odoom Franklin Dodzi 
Published: July 2023

PurposeThis paper seeks to examine the interrelationship between inclusive business, private sector credit and economic welfare in Africa.Design/methodology/approachThe study uses the seemingly unrelated regression, system generalized method of moments and bootstrap quantile regression in a panel of 54 economies in Africa, over the period 2006–2020.FindingsThe authors show that countries that provide more credit to the private sector have better incentives to enhance the ease of doing business. The authors find that ease of doing business and domestic credit to the private sector have a positive and significant effect on economic welfare at higher quantile levels. The authors find that ease of doing business substitutes private sector credit to boost economic welfare, while business account complements private sector credit to boost economic welfare. The authors show that the marginal effect of inclusive business on economic welfare is greater in countries that provide more credit to the private sector.Practical implicationsThe implication is that countries that focus on developing their private sector (through credit expansion) should be able to encourage or facilitate the inclusion of businesses to achieve a sustainable economic welfare.Social implicationsThe implication is that policymakers should be able to develop their business environment through inclusive financing so as to build business confidence in the society.Originality/valueThe paper examines the interrelationship between inclusive business, private sector credit and economic welfare in Africa.
Men are from Mars and women are from Venus: dyadic collaboration in the metaverse
Authors: Schiller Shu , Nah Fiona Fui-Hoon , Luse Andy , Siau Keng 
Published: July 2023

PurposeThe gender composition of teams remains an important yet complex element in unlocking the success of collaboration and performance in the metaverse. In this study, the authors examined the collaborations of same- and mixed-gender dyads to investigate how gender composition influences perceptions of the dyadic collaboration process and outcomes at both the individual and team levels in the metaverse.Design/methodology/approachDrawing on expectation states theory and social role theory, the authors hypothesized differences between dyads of different gender compositions. A blocked design was utilized where 432 subjects were randomly assigned to teams of different gender compositions: 101 male dyads, 59 female dyads and 56 mixed-gender dyads. Survey responses were collected after the experiment.FindingsMultilevel multigroup analyses reveal that at the team level, male dyads took on the we-impress manifestation to increase satisfaction with the team solution. In contrast, female and mixed-gender dyads adopted the we-work-hard-on-task philosophy to increase satisfaction with the team solution. At the individual level, impression management is the key factor associated with trust in same-gender dyads but not in mixed-gender dyads.Originality/valueAs one of the pioneering works on gender effects in the metaverse, our findings shed light on two fronts in virtual dyadic collaborations. First, the authors offer a theoretically grounded and gendered perspective by investigating male, female and mixed-gender dyads in the metaverse. Second, the study advances team-based theory and deepens the understanding of gender effects at both the individual and team levels (multilevel) in a virtual collaboration environment.
Open innovation networks: a driver for knowledge mobilisation in schools?
Authors: Pietsch Marcus , Brown Chris , Aydin Burak , Cramer Colin 
Published: July 2023

PurposeIn organisational and innovation research, the term “open innovation” refers to the inflow and outflow of knowledge to and from organisations: with open innovation theory suggesting active exchanges of knowledge with external actors leads to the development of exploitable new ideas. In the field of education, however, the exchange of knowledge with external parties represents a paradigm shift. In response, this article presents findings from research design to explore the nature and composition of school innovation networks, and the effects of such these networks on knowledge mobilisation.Design/methodology/approachThe study draws on data from a representative random sample of 411 German school leaders. Respondents were asked to detail their engagement in open and closed innovation activity and their school's external collaborations during the last 12 months. A latent class distal outcome model was developed to examine whether different types of collaboration associate with different knowledge mobilisation processes.FindingsThe study findings suggest that schools in Germany mainly use internal knowledge for innovation, with external knowledge exchange taking place on a very limited basis. Knowledge mobilisation varies depending on the innovation network. The authors use the findings to indicate new insights for how schools can further innovate learning and teaching in future.Originality/valueAlthough there is increasing discussion on Professional Learning Networks in schools, the discourse on knowledge mobilisation within educational networks is limited, making concept of open innovation so far completely absent from discourses on school improvement. This paper initiates the population of this new research space.
Sustainable entrepreneurial ecosystems: interdependencies of infrastructure and capital and the effects of local culture
Authors: Weerasekara Sumedha , Bhanugopan Ramudu 
Published: July 2023

PurposeSustainable entrepreneurial ecosystem research is an emerging trend within the entrepreneurship domain. Drawing from resource dependency theory, this study examines the interdependent nature of sustainable entrepreneurial ecosystem factors and the mediating role of local culture as it relates to entrepreneurial action. The authors collected data from 12 entrepreneurial ecosystems in Australia and developed a model of the interdependencies of sustainable entrepreneurial ecosystem factors.Design/methodology/approachThe data were collected through an e-survey of Small and Medium Entreprise (SME) owners in New South Wales, Australia. The authors applied partial least squares structural equation methodology to assess the structural models, validate the outer models and examine the inner model.FindingsThe findings reinforce empirical support for sustainable entrepreneurial ecosystems. The environment where sustainable entrepreneurial ecosystems are evolved influences their functionality. Further, entrepreneurial culture mediates the relationship with other ecosystem factors. The theoretical and practical implications are discussed.Originality/valueThis study focuses on understanding the interdependent nature of sustainable ecosystem factors. The authors identified entrepreneurial culture as a mediator to business support services, educational institutional support and financial capital availability with business and social networks.
A mixed-methods approach for the relationship between organizational attributes, reputation, employer brand and intention to apply for a job vacancy
Authors: Elbendary Israa , Elsetouhi Ahmed Mohamed , Marie Mohamed , Aljafari Abdullah M. 
Published: July 2023

PurposeThis study aims to investigate the direct effect of organizational reputation (OR), employer brand (EB) and organizational attributes (OA) on the intention to apply for a job vacancy (IAJV); further, it examined the mediating effect of employer brand in the OA-IAJV relationship while taking into consideration the moderating effect of organizational reputation.Design/methodology/approachA mixed-method approach was employed, with ten in-depth interviews followed by a questionnaire with additional 356 job seekers in Cairo and Giza cities; the sample includes both fresh graduates and experienced job applicants in the job market. The qualitative analysis confirmed that some respondents use organizational reputation as a signal of its performance. The path analysis technique tests the research hypotheses using a partial least squares structural equation modeling (PLS-SEM).FindingsThe findings revealed that the most influential variable in the intention to apply is organizational attributes, followed by organizational reputation and finally employer brand. There is a significant relationship between organizational attributes and intention to apply for a job vacancy via employer brand. In addition, the results indicate a noteworthy moderating impact of organizational reputation on the association between employer brand intentions to apply for a job and the relationship between organizational attributes and intention to apply for a job opening.Originality/valueTo the best of the authors’ knowledge, this study contributes to the understanding of the direct and indirect effects of organizational reputation and organizational attributes on intention to apply through the mediating role of the employer brand. This research opens new avenues for recruitment research, considering the moderating effect of organizational reputation on strengthening the impact of the independent variables on the intention to apply and the interaction between the variables affecting the intention. Further, this study focuses on the needs of the job applicants when perceiving the organizational factors and identifies which signals can generate the intention to apply according to the signaling theory.
Leveraging digital technologies for circular economy in construction industry: a way forward
Authors: Rodrigo Navodana , Omrany Hossein , Chang Ruidong , Zuo Jian 
Published: July 2023

PurposeThis study aims to investigate the literature related to the use of digital technologies for promoting circular economy (CE) in the construction industry.Design/methodology/approachA comprehensive approach was adopted, involving bibliometric analysis, text-mining analysis and content analysis to meet three objectives (1) to unveil the evolutionary progress of the field, (2) to identify the key research themes in the field and (3) to identify challenges hindering the implementation of digital technologies for CE.FindingsA total of 365 publications was analysed. The results revealed eight key digital technologies categorised into two main clusters including “digitalisation and advanced technologies” and “sustainable construction technologies”. The former involved technologies, namely machine learning, artificial intelligence, deep learning, big data analytics and object detection and computer vision that were used for (1) forecasting construction and demolition (C&D) waste generation, (2) waste identification and classification and (3) computer vision for waste management. The latter included technologies such as Internet of Things (IoT), blockchain and building information modelling (BIM) that help optimise resource use, enhance transparency and sustainability practices in the industry. Overall, these technologies show great potential for improving waste management and enabling CE in construction.Originality/valueThis research employs a holistic approach to provide a status-quo understanding of the digital technologies that can be utilised to support the implementation of CE in construction. Further, this study underlines the key challenges associated with adopting digital technologies, whilst also offering opportunities for future improvement of the field.
Pressure sensitive adhesives and paper spray‐mass spectrometry for the collection and analysis of fentanyl‐related compounds from shipping materials
Authors: Prunty Sarah , Carmany Daniel , Dhummakupt Elizabeth S. , Manicke Nicholas E. 
Published: July 2023

AbstractThe rise of fentanyl and fentanyl analogs in the drug supply pose serious threats to public health. Much of these compounds enter the United States through shipping routes. Here we provide a method for fentanyl screening and analysis that utilizes pressure‐sensitive adhesive (PSA) lined paper to recover drug residues from parcel‐related surfaces. The paper used is commercially available repositionable notes (also called post‐it or sticky notes). From this paper, mass spectra were obtained by paper spray‐mass spectrometry (PS‐MS), where PSA paper served as both a sampling and analysis substrate. Seven fentanyl‐related compounds were analyzed: fentanyl, 4‐anilino‐N‐phenethylpiperidine (4‐ANPP), N,1‐diphenethyl‐N‐phenylpiperidin‐4‐amine (phenethyl‐4‐ANPP), valerylfentanyl, 4‐fluoroisobutyrylfentanyl (4‐FIBF), carfentanil, and p‐fluorofentanyl. These compounds were recovered by PSA paper and identified by PS‐MS from packaging tape and plastic at 50 ng and from cardboard and shipping labels at 100 ng. The impact of cutting agents on PS‐MS analysis of fentanyl analogs was explored. No trends of analyte suppression were found at high concentrations of the cutting agents caffeine, diphenhydramine, and lidocaine when recovered from surfaces. A cartridge that required no precise cutting of PSA paper prior to sampling or analysis was evaluated for use in PS‐MS for fentanyl screening. Recovery and detection of fentanyl from plastic sheeting was demonstrated with this cut‐free cartridge. The cut‐free cartridge showed somewhat less consistency and lower analyte signal than the standard cartridge, but performance was suitable for potential screening applications. In combining PSA surface sampling with PS‐MS for drug screening, both sampling and detection of fentanyl‐related compounds is simple, rapid, and low‐cost.
Less Livestock in North‐western Europe? Discourses and Drivers Behind Livestock Buyout Policies
Authors: Boezeman Daan , de Pue David , Graversgaard Morten , Möckel Stefan 
Published: July 2023

SummaryDirect intervention in the size of livestock numbers is not considered a main option in European agri‐environmental policies nor in policy studies. Nevertheless, the governments of the Netherlands and Flanders (Belgium) have announced livestock buyout schemes. This article contributes to the scarce literature on this policy instrument by sketching the characteristics of different types of buyout schemes. We analyse how the issue of reducing livestock numbers is being framed in four EU Member States with high livestock dense regions: the Netherlands, Belgium (Flanders), Denmark and Germany. While the debate on ‘technology versus volume’ can be observed in all four countries, the ‘nitrogen crises’ in the first two has led to a reframing of concerns over livestock numbers in relation to place‐based deterioration of habitats and the possibility of granting permits for new economic activities, rather than as a global issue of mitigating climate change. Pre‐existing institutional frameworks influence the introduction and design of new buyout policies. In the context of high political pressure, existing policies to close down farms were reinforced and nutrient emission rights systems offered the opportunity to take production rights out of the market. Notwithstanding the policies and available budgets, the issue of direct intervention to reduce livestock numbers remains controversial.