bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fecal Microbiota Restoration Modulates the Microbiome in Inflammation-Driven Colorectal Cancer
Authors: Gates Travis J , Yuan Ce , Shetty Mihir , Kaiser Thomas , Nelson Andrew C , Chauhan Aastha , Starr Timothy K , Staley Christopher , Subramanian Subbaya 
Published: December 2022

ABSTRACTChronic inflammation of the colon (colitis) is a known risk factor for inflammatory-driven colorectal cancers (idCRC), and intestinal microbiota has been implicated in the etiology of id-CRC. Manipulation of the microbiome is a clinically viable therapeutic approach to limiting id-CRC. To understand the microbiome changes that occur over time in id-CRC, we used a mouse model of idCRC with the treatment of azoxymethane (AOM) and dextran sodium sulfate (DSS) and measured the microbiome over time using 16S rRNA amplicon sequencing. We included cohorts where the microbiome was restored using cage bedding swapping and where the microbiome was depleted using antibiotics to compare to untreated animals. We identified consistent increases inAkkermansiain mice receiving horizontal microbiome transfer (HMT), while the control cohort had consistent longitudinal increases inAnaeroplasmaandAlistipes. Additionally, fecal lipocalin-2 (Lcn-2) concentrations were elevated in unrestored animals compared to restored and antibiotic-treated counterparts following HMT. These observations suggest a potential role forAkkermansia, Anaeroplasma, and Alistipesin regulating colonic inflammation in id-CRC.
Data-driven model of glycolysis shows that allostery maintains high ATP levels while mass action controls flux and energy of ATP hydrolysis
Authors: Choe Mangyu , Einav Tal , Phillips Rob , Titov Denis V. 
Published: December 2022

ABSTRACTGlycolysis is a conserved metabolic pathway that produces ATP and biosynthetic precursors. Here, we use mathematical modeling to investigate how the control of mammalian glycolytic enzymes through allostery and mass action accomplishes various tasks of ATP homeostasis, such as controlling the rate of ATP production, maintaining high and stable ATP levels, and ensuring that ATP hydrolysis generates a net excess of energy. Our model uses data-derived enzyme rate equations, recapitulates the key tasks of glycolytic ATP homeostasis, and accurately predicts absolute concentrations of glycolytic intermediates and isotope tracing kinetics in live cells. We find that allosteric regulation of hexokinase (HK) and phosphofructokinase (PFK) by ATP, ADP, inorganic phosphate and glucose-6-phosphate (G6P), the surplus of lower glycolysis enzymes, and a large non-adenine phosphate pool are essential to robustly maintain high ATP levels and to prevent uncontrolled accumulation of phosphorylated intermediates of upper glycolysis. Meanwhile, mass action alone is sufficient to control ATP production rate and maintain high energy of ATP hydrolysis. Our results suggest a revision of the textbook view that the function of allosteric regulation of HK, PFK and PK is to control the net flux through glycolysis in response to variable ATP demand.
An efficient method to generate fluorescent amyloid fibrils
Authors: Prajapati Kailash Prasad , Ansari Masihuzzaman , Yadav Deepak Kumar , Anand Bibin Gnanadhason , Mittal Shikha , Kar Karunakar 
Published: December 2022

ABSTRACTStudies on fluorophore-tagged peptides help in elucidating the molecular mechanism of amyloidogenesis including their cellular internalization and crosstalk potential. Despite several advantages, unavoidable difficulties including expensive and tedious synthesis-protocols exist in fluorophore-based tools. Importantly, covalently-tagged fluorophores could introduce structural constraints which may influence the conformation of the monomeric and aggregated forms of protein. To resolve this problem, we describe a robust yet simple method to make fluorescent amyloid fibrils through non-covalent incorporation of fluorophores into amyloid fibrils. We used aggregation protocol in which a small amount of fluorophore is incorporated into the amyloids, and this protocol does not alter the aggregation kinetics and the characteristic β-sheet-conformers of the generated amyloid fibrils. We have successfully prepared fluorescent amyloid fibrils of Insulin, Lysozyme and Aβ1-42, and the noncovalently incorporated fluorophores remained intact in the amyloid fibrils without leaching, even after serial-dilutions and prolonged-storage. Further, this method enables successful monitoring of cellular-internalization of the fluorescent amyloids into SH-SY5Y and A549 cells, and it also detects FRET-signals during interfibrillar interactions. The findings establish a simple and affordable protocol to prepare fluorescent amyloid structures, which may significantly help amyloid researchers working on bothin vitroand animal model systems.
Metabolic Engineering OfLactococcus LactisFor The Production Of Heparosan
Authors: Guhan Siddharth , Raj Naveen , Sivaprakasam Senthilkumar , Jeeva Pandeeswari 
Published: December 2022

AbstractHeparosan is a precursor molecule for the widely used anticoagulant heparin, which also has other uses such as certain drug delivery applications and as a scaffold for tissue engineering in biomaterials. Traditionally, pathogenic bacteria such asE.Colihave been used as a host to produce heparosan as an alternative to animal and chemoenzymatic synthesis. Using GRAS status organisms likeLactococcus Lactisas the host for production of heparosan provides a safe alternative as well as being a well-established organism for genetic manipulation and reengineering. In this study, a functional heparosan synthesis pathway was successfully expressed inLactococcus Lactisby the expression ofE.coliK5 genes KfiA and KfiC, along with the overexpression of ugd, glmu and pgma genes present natively in the host organism. The genes were activated using the tightly controlled NICE expression system. The genes were cloned into plasmid p8148 and transformed into two strains,Lactococcus LactisNZ9000 andLactococcus LactisNZ9020, totaling six different recombinant strains were created using these two hosts and various combinations of the heterologous genes. The recombinantLactococcus LactisSH6 strain, expressing the genesugd-KfiA-KfiC-pgma yielded a maximum concentration of 754 mg/l in batch bioreactor experiments and the titer was increased to 1263 mg/l in fed-batch fermentation. NMR imaging successfully determined that the structure of the product derived fromLactococcus Lactiswas indeed similar toE.coliheparosan. The molecular weight of heparosan varied from 10-20 KDa, indicating its potential use for chemoenzymatic heparin biosynthesis.
Pre-clinical in vitro and in vivo characterization of a maternal vaccination before conception to protect against severe neonatal infections caused byEscherichia coliK1
Authors: Sereme Youssouf , Schrimp Cécile , Lefebvre-Wloszczowski Esther , Agapoff Maeva , Faury Helène , Marchand Yunhua Chang , Agiron-Ardila Elisabeth , Panafieu Emilie , Blec Frank , Coureuil Mathieu , Frappy Eric , Bonacorsi Stephane , Skurnik David 
Published: December 2022

AbstractPreterm birth remains the leading cause of neonatal morbidity and mortality today. Genetic, immunological, and infectious substrates are suspected. Preterm infants are at higher risk of severe neonatal infections and the main cause of bacterial infection in this population isEscherichia coliK1. Unfortunately, women with history of preterm birth have a high risk of recurrence. Therefore, these women constitute a target population for a vaccine, to date non-existent, againstE. coliK1 to prevent these infections.In this study, we characterize the immunological and microbiological properties in adult female mice of a live attenuated vaccine candidate and the protection it conferred to newborn mice against severe infection caused byE. coliK1. We show that ourE. coliK1 ΔaroA vaccine induces a strong immunity driven by polyclonal bactericidal antibodies. In our model of meningitis, pups born from mothers immunized before conception were strongly protected against different strains ofE. coliK1 both in early-onset and late-onset diseases.Given the very high rate of mortality and neurological sequalae in neonatal meningitis caused byE. coliK1, this pre-clinical study provides a proof-of-concept for the development of a vaccine strategy againstE. coliK1 severe infection in women at risk of preterm birth.
Understanding prefrontal cortex functions by decoding its molecular, cellular and circuit organization
Authors: Bhattacherjee Aritra , Zhang Chao , Watson Brianna , Djekidel Mohamed Nadhir , Moffitt Jeffrey R. , Zhang Yi 
Published: December 2022

AbstractThe prefrontal cortex (PFC) is functionally one of the most complex regions of mammalian brain. Unlike other cortical areas that process single sensory modalities (like vision, touch, smell, etc.), the PFC integrates information across brain regions to regulate diverse functions ranging from cognition, emotion, executive action to even pain sensitivity. However, it is unclear how such diverse functions are organized at the cellular and circuit levels within the anatomical modules of the PFC. Here we employed spatially resolved single-cell transcriptome profiling to decode PFC’s organizational heterogeneity. The results revealed that PFC has very distinct cell type composition relative to all neighboring cortical areas. Interestingly, PFC also adopts specialized transcriptional features, different from all neighbors, with differentially expressed genes regulating neuronal excitability. The projections to major subcortical targets of PFC emerge from combinations of neuron subclusters determined in a target-intrinsic fashion. These cellular and molecular features further segregated within subregions of PFC, alluding to the subregion-specific specialization of several PFC functions. Finally, using these unique cellular, molecular and projection properties, we identified distinct cell types and circuits in PFC that engage in pathogenesis of chronic pain. Collectively, we not only present a comprehensive organizational map of the PFC, critical for supporting its diverse functions, but also reveal the cluster and circuit identity of a pathway underlying chronic pain, a rapidly escalating healthcare challenge limited by molecular understanding of maladaptive PFC circuits.Major pointsPFC adopts unique cellular composition, distinct from other cortical areasSelective transcriptomic features emerge in PFC to support its divergent functional portfolioSubcortical projections of PFC assume target-intrinsic specification for innervating clustersA molecularly defined L5 projection neuron cluster (to PAG) potentially mediates chronic pain pathogenesis
Distinct spatial patterns of neural and physiology-related activity in the human periaqueductal grey during anticipatory social stress
Authors: Theriault J.E. , Fischbach A. , Kragel P.A. , Wager T.D. , Wald L.L. , Bianciardi M. , Choi J.-K. , Zhang J. , Quigley K.S. , Barrett L.F. , Satpute A.B. 
Published: December 2022

AbstractPeriaqueductal gray (PAG) columns mediate affective experience, physiological regulation, and survival-related behavior; yet, only 7T imaging can resolve these small structures in humans. In a social stress task, participants prepared a speech, and we observed (a) bilateral ventrolateral PAG activity, relative to baseline, and (b) distinct spatial patterns of correlation between PAG activity and physiological response (i.e., cardiac interbeat interval, reparation rate, and tonic electrodermal activity).
A case report on cataract surgery in eyes with bilateral inferior iridoschisis
Authors: Alzuhairy Sultan 
Published: December 2022

PurposeThe purpose of the paper is to report a case of bilateral inferior iridoschisis who underwent cataract surgery with intraocular lens implantation successfully with the help of iris hooks or pupillary expanders.Design/methodology/approachA 71-year-old male presented with inferior iridoschisis in both eyes, history of angle closure glaucoma (ACG), cataract and shallow anterior chamber (AC) angles inferiorly. A localized area of iris stroma is cleaved in two with anterior atrophic portion disintegrating into fibrils from the posterior stroma, and muscle layer is termed as iridoschisis. Iridoschisis is a rare condition associated with fibrillary iris degeneration, narrow drainage angles and cataract.FindingsPreoperative and postoperative ocular examination, including visual acuity, intraocular pressure and degrees of iris damage, was evaluated. Cataract surgery was performed under topical anesthesia with flexible iris hooks. There were no intraoperative complications whereas marked corneal edema was shown at immediate postoperative period but subsided completely in two weeks’ time. Visual acuity improved from 20/60 to 20/25.Practical implicationsThis case report demonstrates that while iridoschisis care during cataract surgery has been reported to be difficult, cataract extraction was managed using iris hooks.Originality/valueThis paper reports the successful management of cataract in a patient with bilateral inferior iridoschisis.
Exploratory study of the impact of the supply chain on integrated product-service systems design
Authors: Barrar Hicham , Ruiz-Benitez Rocio 
Published: December 2022

PurposeManufacturing firms (MFs) nowadays are struggling to design and maintain an integrated product-service offer. This paper aims to explore the role of the supply chain (SC) in the design of the Product-Service System (PSS). In particular, the study focuses on the Design for Supply Chain (DfSC) approach in order to understand its role and contribution to the Design for Product Service Supportability (DfPSSu) approach in supporting PSS design.Design/methodology/approachBased on the multiple-case study approach, this paper used in-depth-interviews method to collect primary data from four multinational car manufacturers and two academic researchers.FindingsThe study reveals how a better design of the SC is required for the development of a service supportability approach that, in turn, facilitates the design of the PSS. Additionally, Internet of Things (IoT) technologies support MFs to analyse the ongoing development of the PSS business model. Finally, a better design of PSS is essential for strengthening the integration of Product and Service Offerings.Practical implicationsThis study suggests that MFs can build dynamic SC capabilities to deal with fundamental changes that occurred when adopting servitization.Originality/valueThis paper is among the first attempts to study the design process of the PSS business model in a real business context taking into account different design strategies.
Greenhouse gas emissions and stock market volatility: an empirical analysis of OECD countries
Authors: Noh Jung Hee , Park Heejin 
Published: December 2022

PurposeThis study aims to explore empirical evidence of the impact of greenhouse gas (GHG) emissions on stock market volatility.Design/methodology/approachUsing panel data of 35 Organization for Economic Co-operation and Development countries from 1992 to 2018, we conduct both fixed effects panel model and Prais-Winsten model with panel-corrected standard errors.FindingsThe authors document that there is a significant positive relationship between GHG emissions and stock market volatility. The results remain robust after controlling for potential endogeneity problems.Originality/valueThis study contributes to the literature in that it provides additional empirical evidence for the financial risk posed by climate change.
Do family companies invest more in internal audit function (IAF) than non-family companies?
Authors: Ali Al-Qadasi Adel 
Published: December 2022

PurposeMotivated by the growing interest of governance regulators and researchers on internal audit function (IAF), this study examines the influence of family ownership on the levels of investment in IAF.Design/methodology/approachA sample of Malaysian listed companies for the period 2009 to 2016 is used. To test our hypothesis, the authors use pooled panel data regression based on two-way cluster-robust standard errors (firm and year).FindingsThe findings show that family ownership is negatively related to investment in IAF; in particular, investment in IAF is lower for family companies than non-family companies.Originality/valueThis study contributes to existing knowledge of IAF, and it provides significant insights for regulators and managers into the variation in governance structures between family and non-family companies, particularly in emerging markets in which substantial family ownership is common.
The effect of empowering leadership on job performance among frontline employees: does power distance orientation matter?
Authors: Vuong Bui Nhat , Hieu Vo Thi 
Published: December 2022

PurposeThe objective of this research is to investigate how empowering leadership (EL) could promote employees' knowledge sharing (KS) and innovative work behavior (IWB), which, in turn, improve their job performance (JP). Besides, the moderating role of power distance orientation (PDO) will be considered as well.Design/methodology/approachThis study employed a self-reported questionnaire from 460 frontline employees at Vietnamese commercial banks and the partial least squares structural equation modeling (PLS-SEM) via the SmartPLS 3.0 to test the hypotheses.FindingsThe findings showed that there was a positive influence of EL on JP; this was mediated partially through KS and IWB. Additionally, the first research was the one that revealed that employees with high PDO dampened the positive effects of EL on KS and IWB.Practical implicationsThe result of this study suggests that managers should evolve EL to heighten employees' JP.Originality/valueThe findings explored correlations between a system of variables. Besides, the main results of this research also demonstrated that EL played a crucial role in KS, IWB and JP.
Bi-objective optimization-based multi-criteria decision-making framework for disassembly line balancing and employee assignment problem
Authors: Deniz Nurcan , Ozcelik Feristah 
Published: December 2022

PurposeAlthough disassembly balancing lines has been studied for over two decades, there is a gap in the robotic disassembly. Moreover, combination of problem with heterogeneous employee assignment is also lacking. The hazard related with the tasks performed on disassembly lines on workers can be reduced by the use of robots or collaborative robots (cobots) instead of workers. This situation causes an increase in costs. The purpose of the study is to propose a novel version of the problem and to solve this bi-objective (minimizing cost and minimizing hazard simultaneously) problem.Design/methodology/approachThe epsilon constraint method was used to solve the bi-objective model. Entropy-based Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) and Preference Ranking Organization methods for Enrichment Evaluation (PROMETHEE) methods were used to support the decision-maker. In addition, a new criterion called automation rate was proposed. The effects of factors were investigated with full factor experiment design.FindingsThe effects of all factors were found statistically significant on the solution time. The combined effect of the number of tasks and number of workers was also found to be statistically significant.Originality/valueIn this study, for the first time in the literature, a disassembly line balancing and employee assignment model was proposed in the presence of heterogeneous workers, robots and cobots to simultaneously minimize the hazard to the worker and cost.
The future of public sector accounting research. A polyphonic debate
Authors: Grossi Giuseppe , Steccolini Ileana , Adhikari Pawan , Brown Judy , Christensen Mark , Cordery Carolyn , Ferry Laurence , Lassou Philippe , McDonald III Bruce , Raudla Ringa , Sicilia Mariafrancesca , Vinnari Eija 
Published: December 2022

PurposeThe purpose of this polyphonic paper is to report on interdisciplinary discussions on the state-of-the-art and future of public sector accounting research (PSAR). The authors hope to enliven the debates of the past and future developments in terms of context, themes, theories, methods and impacts in the field of PSAR by the exchanges they include here.Design/methodology/approachThis polyphonic paper adopts an interdisciplinary approach. It brings into conversation ideas, views and approaches of several scholars on the actual and future developments of PSAR in various contexts, and explores potential implications.FindingsThis paper has brought together scholars from a plurality of disciplines, research methods and geographical areas, showing at the same time several points of convergence on important future themes (such as accounting as a mean for public, accounting, hybridity and value pluralism) and enabling conditions (accounting capabilities, profession and digitalisation) for PSA scholarship and practice, and the richness of looking at them from a plurality of perspectives.Research limitations/implicationsExploring these past and future developments opens up the potential for interesting theoretical insights. A much greater theoretical and practical reconsideration of PSAR will be fostered by the exchanges included here.Originality/valueIn setting out a future research agenda, this paper fosters theoretical and methodological pluralism in the interdisciplinary research community interested in PSAR in various contexts. The discussion perspectives presented in this paper constitute not only a basis for further research in this relevant accounting area on the role, status and developments of PSAR but also creative potential for practitioners to be more reflective on their practices and also intended and united outcomes of such practices.
Battles over ‘unruly bodies’: Practitioners’ interpretations of eating disorders and the utility of psychiatric labelling
Authors: Fixsen Alison , Ridge Damien , Ponsford Olivia , Holder Mesha , Saran Gurjeet 
Published: December 2022

AbstractOur article offers a critical appraisal of psychiatric medical constructions of eating disorders (EDs) by highlighting the complexity of professional discourses, power plays, claims and counterclaims in ongoing struggles over ‘unruly bodies.’ Inductive thematic analysis of data from five studies was undertaken, covering thirty semi‐structured interviews with UK health‐care professionals working in the ED field. Professionals engaged in various struggles over eating disorders, which were compounded by the labelling process itself. Although person‐centredness was valued, encounters with ED patients were frequently framed as outstandingly problematic in terms of treatment resistance, with clients’ unruliness spurred on by ED competitiveness on social media and in‐hospital rivalries. Paradoxically, the labelling of eating disorders created further expectations in terms of achieving specific weight levels and diagnostic labels, reportedly resulting in lay competition over who was sickest, which was mirrored by treatment priorities within an overstretched public health system. Narratives also highlighted interesting tensions between professionals rationalising their work with patients and acknowledging themselves as potentially vulnerable to societal pressures promoting EDs. Arguably, while lay narratives around EDs continue to be framed by authorities as unruly behaviours in deviant individuals, the gap between officially sanctioned and illicit ED discourses will only grow.