bluuebunny/crossref_metadata_2025_split huggingface dataset:
Teachers' perception of aggressive student behavior through the lens of chronic worry and resignation, and its association with psychophysiological stress: An observational study
Authors: Wettstein Alexander , Jenni Gabriel , Schneider Sandra , Kühne Fabienne , grosse Holtforth Martin , La Marca Roberto 
Published: April 2023

AbstractAggressive student behavior is considered a leading risk factor for teacher stress. However, teachers' coping styles may affect how they perceive and respond to aggressive student behavior. This study tests whether teachers' perceptions of aggressive student behavior mainly mirror objectively observed aggression in presence of the teacher (as coded by external observers) or whether teachers' perception of aggressive student behavior primarily reflects teachers' avoidant coping styles, such as chronic worry and resignation. Finally, we examine whether observed and teacher-perceived aggression relates to increased vital exhaustion and psychophysiological stress among teachers (i.e., higher hair cortisol concentration). In an ambulatory assessment study, we administered self-reports to 42 Swiss teachers to assess perceived student aggression, chronic worry, resignation, and vital exhaustion. Additionally, four consecutive lessons per teacher were filmed, and aggressive student behavior in presence of the teacher was coded by four trained external observers. The concentration of cortisol was assessed in hair samples. Results showed that teacher-perceived and observed aggression were moderately associated. Observed aggression was related to teacher perceptions to a much lesser extent than teachers' avoidant coping styles, that is, chronic worry and resignation. While teacher-perceived student aggression was associated with teachers' self-reported vital exhaustion, we did not find any significant association with hair-cortisol concentration. Our findings suggest that teachers perceive student aggression through the lens of their coping styles. Teachers' dysfunctional coping styles are associated with an overestimation of student aggression. Teachers' overestimation of student aggression relates to higher levels of vital exhaustion. Therefore, it is crucial to identify and change teachers' dysfunctional coping styles to prevent a vicious cycle of dysfunctional teacher–student interactions.
The conjunctival fungal microflora of horses in a North Queensland tropical environment and their in vitro susceptibilities to antifungal agents
Authors: Mo Phu Mo , Picard Jacqueline , Gummow Bruce 
Published: April 2023

AbstractFungi are ubiquitous in the environment and part of the commensal microflora on the conjunctiva of equine eyes. North Queensland, being tropical, presents an ideal environment for fungi growth. When the cornea is injured, fungi can invade the corneal stroma, resulting in keratomycosis. The objectives of this study were to determine the fungal species specific to the eyes of horses in the Townsville region; to investigate the potential risk factors associated with the presence of fungi; and to test their susceptibility to antifungals to create an empirical guide for treatment. The eyes of forty ophthalmologically normal horses from James Cook University were sampled throughout the summer months of December 2017, January 2018, and January and February 2020. Cultured fungi were identified morphologically, and their identity confirmed by comparing partial 18sRNA DNA sequences with the NCBI nucleotide database. Minimum inhibitory concentration testing of common antifungal medications was performed. Sixty-one out of eighty conjunctival samples grew fungi, and 21 different fungi genera were isolated. The most common genera wereAspergillus(18%, 26/141),Curvularia(14%,20/141),Rhodotorula(12%,17/141) andPenicillium(12%,17/141). No significant association was found between age or environmental factors and fungal culture status. Most fungi were highly susceptible to voriconazole and ketoconazole but resistant to fluconazole and amphotericin B. This adds to the body of evidence on which species of fungi are present as normal ocular microflora of horses living in tropical regions of Australia, and an avenue for treating them.
In vivo Assessment of the Impact of Molecular Weight on Constructs of 68Ga-DOTA-Manocept in a Syngeneic Mouse Tumor Model
Authors: Bartels Jennifer L. , Fernandez Solana R. , Arnold Jeffrey S. , Parker Candace C. , Tekin Volkan , O’Malley Grace , Ralph David A. , Lapi Suzanne E. 
Published: April 2023

Abstract

Purpose
Manocept™ constructs are mannosylated amine dextrans (MADs) that bind with high affinity to the mannose receptor, CD206. Tumor-associated macrophages (TAMs) are the most numerous immune cells in the tumor microenvironment and a recognized target for tumor imaging and cancer immunotherapies. Most TAMs express CD206, suggesting utility of MADs to deliver imaging moieties or therapeutics to TAMs. The liver Kupffer cells also express CD206, making them an off-target localization site when targeting CD206 on TAMs. We evaluated TAM targeting strategies using two novel MADs differing in molecular weight in a syngeneic mouse tumor model to determine how varying MAD molecular weights would impact tumor localization. Increased mass dose of the non-labeled construct or a higher molecular weight (HMW) construct were also used to block liver localization and enhance tumor to liver ratios.

Procedures
Two MADs, 8.7 kDa and 22.6 kDa modified with DOTA chelators, were synthesized and radiolabeled with 68Ga. A HMW MAD (300 kDa) was also synthesized as a competitive blocking agent for Kupffer cell localization. Balb/c mice, with and without CT26 tumors, underwent dynamic PET imaging for 90 min followed by biodistribution analyses in selected tissues.

Results
The new constructs were readily synthesized and labeled with 68Ga with ≥ 95% radiochemical purity in 15 min at 65 °C. When injected at doses of 0.57 nmol, the 8.7 kDa MAD provided 7-fold higher 68Ga tumor uptake compared to the 22.6 kDa MAD (2.87 ± 0.73%ID/g vs. 0.41 ± 0.02%ID/g). Studies with increased mass of unlabeled competitors showed reduced liver localization of the [68Ga]MAD-8.7 to varying degrees without significant reductions in tumor localization, resulting in enhanced tumor to liver signal ratios.

Conclusion
Novel [68Ga]Manocept constructs were synthesized and studied in in vivo applications, showing that the smaller MAD localized to CT26 tumors more effectively than the larger MAD and that the unlabeled HMW construct could selectively block liver binding of [68Ga]MAD-8.7 without diminishing the localization to tumors. Promising results using the [68Ga]MAD-8.7 show a potential path to clinical applications.

Graphical Abstract
Effects of vegetation distribution along river transects on the morphology of a gravel bed braided river
Authors: Zhu Runye , Tsubaki Ryota , Toda Yuji 
Published: April 2023

AbstractThe interaction between vegetation, sediment, and water flow creates various fluvial landscapes. Hydrological conditions and flood disturbances, as well as the habitat preference of vegetation, regulate its spatial distribution. To describe the spatial distribution of vegetation cover, here, we focus on vegetation distributions along river transverse transects that define vertical and horizontal distances from water areas during low flow periods. As one of the most dynamic river types, braided rivers can be significantly influenced by vegetation encroachment. However, the effects of vegetation distributions along river transects on braided river morphology remain unknown. To study the potential influence of vegetation distribution along river transects, a depth-averaged, hydro-morphodynamic model was employed. Using the model, we investigated a medium-sized, braided river with a gravel bed affected by riparian vegetation. The following scenarios of vegetation transect distributions were examined: (1) vegetation established near or covering the low water channel, and (2) vegetation established on bar tops and kept at a distance from the low water channel. The model successfully reproduced a reduction in the braiding index for a vegetated braided river. Depending on the transect distribution scenarios employed, significantly different effects for river morphology were obtained. For example, compared to vegetation on bar tops, vegetation located near the low water channel played a more critical role for changing river morphology, redirecting water flow, and changing the statistical characteristics of the riverbed elevation distribution. Vegetation near the low water channel not only concentrated water flow to low water channels but also redirected flow to the high elevation area by reducing low water channel flow capacity. The revealed effects of the vegetation transect distribution on river morphology development helped to determine effective management protocols for reducing the negative impact of vegetation encroachment.
A Background of Bias: Subtle Changes in Lineup Backgrounds Increase the Own Race Bias
Authors: Havard Catriona , Breese Emily , Thirkettle Martin , Kask Kristjan , Leol Kris-Loreen , Mädamürk Kaja 
Published: April 2023

AbstractIn police photo lineups, there can sometimes be small variations in shades and hues of the background images due to the faces being filmed under different lighting and cameras. Own race bias refers to a situation where people are better at remembering the faces of those who are the same race as them and find it more difficult to recognise faces from a different race. In this paper, we investigated the influence of small colour variations in backgrounds for the recognition of Black and White faces. Across 3 experiments, we found when small changes were introduced into the backgrounds of the images this increased false identifications for previously unseen Black faces, but not White faces. This finding suggests that the police need to ensure that the backgrounds of the photo lineups they use are all uniform to reduce mistaken identifications of innocent suspects.
Selective Disruption of Perineuronal Nets in Mice Lacking Crtl1 is Sufficient to Make Fear Memories Susceptible to Erasure
Authors: Poli Andrea , Viglione Aurelia , Mazziotti Raffaele , Totaro Valentino , Morea Silvia , Melani Riccardo , Silingardi Davide , Putignano Elena , Berardi Nicoletta , Pizzorusso Tommaso 
Published: April 2023

Abstract
The ability to store, retrieve, and extinguish memories of adverse experiences is an essential skill for animals’ survival. The cellular and molecular factors that underlie such processes are only partially known. Using chondroitinase ABC treatment targeting chondroitin sulfate proteoglycans (CSPGs), previous studies showed that the maturation of the extracellular matrix makes fear memory resistant to deletion. Mice lacking the cartilage link protein Crtl1 (Crtl1-KO mice) display normal CSPG levels but impaired CSPG condensation in perineuronal nets (PNNs). Thus, we asked whether the presence of PNNs in the adult brain is responsible for the appearance of persistent fear memories by investigating fear extinction in Crtl1-KO mice. We found that mutant mice displayed fear memory erasure after an extinction protocol as revealed by analysis of freezing and pupil dynamics. Fear memory erasure did not depend on passive loss of retention; moreover, we demonstrated that, after extinction training, conditioned Crtl1-KO mice display no neural activation in the amygdala (Zif268 staining) in comparison to control animals. Taken together, our findings suggest that the aggregation of CSPGs into PNNs regulates the boundaries of the critical period for fear extinction.
Zugänge zu Ombudsstellen niedrigschwellig schaffen
Authors: Thomas Severine , Hagemeier Yannick , Rusack Tanja , Taphorn Mara 
Published: April 2023

ZusammenfassungDie Praxis der Kinder- und Jugendhilfe zeigt, dass es bei der Leistungsbewilligung Konflikte zwischen den daran Beteiligten geben kann. Durch das Kinder- und Jugendstärkungsgesetz ist daher seit 2021 ist die Einrichtung ombudschaftlicher Beratungsstellen vorgeschrieben. Damit diese Beratungsstellen für Kinder, Jugendliche und ihre Eltern hilfreich sein können, sollte der Zugang zu ihnen möglichst niedrigschwellig sein. Der Beitrag erörtert, wie Niedrigschwelligkeit konkret aussehen muss, um ihrem Namen gerecht zu werden.
1H, 15N, 13C backbone and Cβ resonance assignments for UBQLN1 UBA and UBAA domains
Authors: Buel Gwen R. , Chen Xiang , Kayode Olumide , Cruz Anthony , Walters Kylie J. 
Published: April 2023

AbstractUBQLN1 functions in autophagy and proteasome-mediated protein degradation. It contains an N-terminal ubiquitin-like domain (UBL), a C-terminal ubiquitin-associated domain (UBA), and a flexible central region which functions as a chaperone to prevent protein aggregation. Here, we report the 1H, 15N, and 13C resonance assignments for the backbone (NH, N, C’, Cα, and Hα) and sidechain Cβ atoms of the UBQLN1 UBA and an N-terminally adjacent segment called the UBA-adjacent domain (UBAA). We find a subset of the resonances corresponding to the UBAA to have concentration-dependent chemical shifts, likely due to self-association. We also find the backbone amide nitrogen of T572 to be shifted upfield relative to the average value for a threonine amide nitrogen, a phenomenon likely caused by T572 Hγ1 engagement in a hydrogen bond with adjacent backbone carbonyl atoms. The assignments described in this manuscript can be used to study the protein dynamics of the UBQLN1 UBA and UBAA as well as the interaction of these domains with other proteins.
How does the learning environment support vocational student learning of domain-general competencies?
Authors: Löfgren Sami , Ilomäki Liisa , Lipsanen Jari , Toom Auli 
Published: April 2023

AbstractStudies and policy reports worldwide argue that a modern employee must possess domain-general competencies to become employed. However, competency studies within upper-secondary initial vocational education and training are scarce. Therefore, this study aimed to scrutinise this topic and examined how the experienced learning environment contributes to student learning of competencies. Study participants were students in automotive engineering, mechanical and metal engineering, electrical and automation engineering and building service technology. The data were collected with an online questionnaire and analysed statistically using structural equation modelling. The research findings indicate firstly that eight competency domains could be recognised: work organisation, cooperation ability, professional attitude, problem solving, willingness to learn, active listening, empathy and assertiveness. Secondly, students’ experienced learning environment was characterised by social support and recognition provided by educators, equal treatment between students and a positive climate for learning. Thirdly, the quality of the experienced learning environment contributed to learning of competencies. The research findings enhance the scientific and societal discussion about vocational graduate competencies and to what extent the experienced learning environment contributes to the learning of competencies.
Consensus for criteria of running a pediatric inflammatory bowel disease center using a modified Delphi approach
Authors: Luo You-You , Wu Kai-Chun , Gong Si-Tang , Huang Ying , Yang Hong , Tang Qing-Ya , Leung Ying-kit , Wu Jie , Geng Lan-Lan , Zhou Wei , Sun Mei , Wan Chao-Min , Li Zai-Ling , Fang Ying , Li Xiao-Qin , Li Mei , Wang Zhao-Xia , Xiao Yuan , Zhong Xue-Mei , Chen Xiao-Fei , Chen Jie 
Published: April 2023

Abstract
Background
Good quality of care for inflammatory bowel disease (IBD) depends on high-standard management and facility in the IBD center. Yet, there are no clear measures or criteria for evaluating pediatric IBD (PIBD) center in China. The aim of this study was to develop a comprehensive set of quality indicators (QIs) for evaluating PIBD center in China.

Methods
A modified Delphi consensus-based approach was used to identify a set of QIs of structure, process, and outcomes for defining the criteria. The process included an exhaustive search using complementary approaches to identify potential QIs, and two web-based voting rounds to select the QIs defining the criteria for PIBD center.

Results
A total of 101 QIs (35 structures, 48 processes and 18 outcomes) were included in this consensus. Structure QIs focused on the composition of multidisciplinary team, facilities and services that PIBD center should provide. Process QIs highlight core requirements in diagnosing, evaluating, treating PIBD, and disease follow-up. Outcome QIs mainly included criteria evaluating effectiveness of various interventions in PIBD centers.

Conclusion
The present Delphi consensus developed a set of main QIs that may be useful for managing a PIBD center.
Vertical segregation, innovation, and gender diversity in Spain’s industrial sector
Authors: Sánchez Rosario , Diaz Ángeles , Urbano Amparo 
Published: April 2023

AbstractInnovation is a general measure of the success of a company and shows a positive relationship with several key factors such as collaboration with universities and technology centers or belonging to medium- and high-tech sectors. While many studies have found that gender diversity in company management teams has a positive influence on companies’ economic success, few have focused on the impact that the inclusion of women in the board of directors has on product, process, and organizational innovation. This paper builds on previous knowledge about the determinants of innovations and shows that greater gender management team diversity, compared to male-only teams, positively affects the achievement of said innovations. However, these results change when we incorporate the size of the companies as a moderating variable. In this case, we identify a turning point corresponding to firm size in the impact of gender diversity on product and process innovations, but not for innovations in organizational methods, where the impact is positive and significant for both SMEs and large enterprises. Collaboration with universities and technological centers is an important determinant of innovation for both SMEs and large enterprises.
Molecular dynamics simulation of pyruvate kinase to investigate improved thermostability of artificially selected strain in Enterococcus faecium
Authors: Li Vladimir , Lee Chul , Lee Youngho , Kim Heebal 
Published: April 2023

Abstract
Background
Enterococcus faecium (E. faecium) is a member of symbiotic lactic acid bacteria in gastrointestinal tract and it was successfully used to treat diarrhea cases in humans. For a lactobacteria to survive during the pasteurization process, resistance of proteins to denaturation at high temperatures is crucial. Pyruvate kinase (PYK) is one of the proteins possessing such property. It plays a major role during glycolysis by producing pyruvate and adenosine triphosphate (ATP).

Objective
To assess the acquired thermostability of PYK of ALE strain using in silico methods.

Methods
First, we predicted and assessed tertiary structures of our proteins using SWISS-MODEL homology modelling server. Second, we then applied molecular dynamics (MD) simulation to simulate and assess multiple properties of molecules. Therefore, we implemented comparative MD to evaluate thermostability of PYK of recently developed high temperature resistant strain of E. faecium using Adaptive Laboratory Evolution (ALE) method. After 20ns of simulation at different temperatures, we observed that ALE enhanced strain demonstrated slightly better stability at 300, 340 and 350 K compared to that of the wild type (WT) strain.

Results
We collected the results of MD simulation at four temperature points: 300, 340, 350 and 400 K. Our results showed that the protein demonstrated increased stability at 340 and 350 K.

Conclusion
Results of these study suggest that PYK of ALE enhanced strain of E. faecium demonstrates overall better stability at elevated temperatures compared to that of WT strain.
Tumour-derived extracellular vesicle based vaccines for melanoma treatment
Authors: Gonzalez-Melero Lorena , Hernandez Rosa Maria , Santos-Vizcaino Edorta , Igartua Manoli 
Published: April 2023

AbstractThe interest of extracellular vesicles (EVs) in cancer immunotherapy is increasing every day. EVs are lipid bilayer vesicles released by most cells, which contain the molecular signature of their parent cell. Melanoma-derived EVs present antigens specific to this aggressive type of cancer, but they also exert immunomodulatory and pro-metastatic activity. Until now, most reviews focus on the immunoevasive characteristics of tumour-derived EVs, but do not help to overcome the issues related to them. In this review, we describe isolation methods of EVs from melanoma patients and most interesting markers to oversee their effect if they are used as antigen carriers. We also discuss the methods developed so far to overcome the lack of immunogenicity of melanoma-derived EVs, which includes EV modification or adjuvant co-administration. In summary, we conclude that EVs can be an interesting antigen source for immunotherapy development once EV obtaining is optimised and the understanding of the mechanisms behind their multiple effects is further understood.
Graphical Abstract
Antimicrobial and anticancer activities of biosynthesized bimetallic silver-zinc oxide nanoparticles (Ag-ZnO NPs) using pomegranate peel extract
Authors: Hashem Amr H. , El-Sayyad Gharieb S. 
Published: April 2023

AbstractIn the last two decades, nanomaterials have received much attention for the treatment of multidrug-resistant microbes that threaten human health. In the current study, the novelty and scientific significance concentrated on the biogenic synthesis of bimetallic silver-zinc oxide nanoparticles (Ag-ZnO NPs) using pomegranate peel extract (PPE) for the first time. The new constructed bimetallic Ag-ZnO NPs possessed the synergistic activity at a low concentration to avoid toxicity and elevate the superior potential. UV-Vis. characterization illustrated that Ag-ZnO NPs were small in size (15.8 nm), which was observed at 395.0 nm. The SEM image of Ag-ZnO NPs, incorporated with PPE, exhibited uniform Ag-ZnO NP surfaces with a clear surface appearance. It can be detected that Ag-ZnO NPs were isolated typically as a rounded particle across the PPE, which showed as brilliant NPs combined and stabilized with the prepared PPE. Results also revealed that Ag-ZnO NPs exhibited potential antibacterial activity toward Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, and Enterococcus faecalis where minimum inhibitory concentrations (MICs) were 62.5, 125, 15.62, 62.5, and 250 µg/ml. Likewise, Ag-ZnO NPs appeared antifungal activity against Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus, and Aspergillus brasiliensis, where MICs were 7.81, 31.25, 125, and 62.5 µg/ml, respectively. Moreover, Ag-ZnO NPs exhibited anticancer activities against MCF7 and Caco2, where IC50 was 104.9 and 52.4 µg/ml, respectively. Additionally, these concentrations are safe in use where results of cytotoxicity on Vero normal cell line confirmed that IC50 was 155.1 µg/ml. Overall, bimetallic Ag-ZnO NPs were for the first time, successfully biosynthesized using PPE; also, they had a promising antibacterial, antifungal, and anticancer activities.