bluuebunny/crossref_metadata_2025_split huggingface dataset:
P21 Tyrosine and serine STAT3 phosphorylation drives cutaneous squamous cell carcinoma in recessive dystrophic epidermolysis bullosa as demonstrated using a 3D in vitro model
Authors: Llopis-Hernandez Virginia , Balon Katarzyna , Chen Dawei , Mellerio Jemima , McGrath John , Jacków Joanna 
Published: July 2023

Abstract
The early onset of aggressive cutaneous squamous cell carcinoma (cSCC) and its rapid progression in recessive dystrophic epidermolysis bullosa (RDEB) leads to nearly 100% mortality rates in these patients. Elevated levels of phospho-STAT3 on tyrosine residue (STAT3Tyr705) have been intensively demonstrated in RDEB-derived cSCC cells, demonstrating that the constitutive activation and dysregulation of the Janus kinase (JAK)/STAT pathway plays a role in RDEB cSCC pathogenesis. We efficiently applied ruxolitinib (JAK1/2 inhibitor that blocks phosphorylation on STAT3Tyr705) to a RDEB–cSCC xenograph mouse model, reducing the size of small tumours but found that it was insufficient to reduce bigger tumours completely. Recently, phosphorylation at serine-727 (STAT3Ser727) has been shown to be relevant in the evolution of some cancers, but it has not yet been studied in RDEB cSCC. We hypothesized that phosphorylation at serine-727 of the STAT3 pathway might play a role in RDEB cSCC tumour development and progression, and could not be inhibited by ruxolitinib. To validate our hypothesis, we studied two RDEB cSCC human patient samples with different degrees of dedifferentiation (normal RDEB skin, edge of the tumour and centre of the RDEB cSCC). We demonstrated that STAT3Tyr705 was preferentially upregulated in the normal RDEB skin followed by the tumour edge skin, presenting less phosphorylation on the tumour. When we analysed STAT3 phosphorylation on STAT3Ser727, we could see that it presented the opposite phosphorylation pattern, being more phosphorylated in the tumour and less in normal RDEB skin. Furthermore, we validated the STAT3Ser727residue pattern across other cSCC cell lines and found that serine phosphorylation is present in other SCCs, demonstrating its crucial role in tumour development. To further investigate and analyse cancer progression in more detail, an easy and robust RDEB cSCC model is instrumental. Therefore, we generated RDEB cSCC spheroids in vitro, which has four key advantages over the previous models developed: (i) it is easy to generate; (ii) small amounts of cells are required; (iii) it is suitable for drug testing; and (iv) it can be used to recapitulate different stages of tumour development. We have been able to control their size and use them for drug testing, observing a 20% size decrease after 6 days of ruxolitinib treatment. Taken together, this three-dimensional RDEB cSCC in vitro model, along with analysis of human samples, will allow us to better understand RDEB cSCC progression at different stages, allowing us to investigate new treatments for RDEB cSCC.
Ontological (In)security in Early Career Social Work during COVID-19: Experiences in Scotland
Authors: Sen Robin , Daly Maura , McCulloch Trish , Grant Scott , Clarke David , Ferrier Claire 
Published: July 2023

Abstract
The impact of COVID-19 on the working lives of professionals has been of much interest. Within social work, the pandemic increased workload demands, whilst the way in which work was done shifted significantly. This article uses data gathered from newly qualified social workers (NQSWs) who began their working lives during the pandemic. These first years in practice are viewed as an extension to social workers’ formal education and as a vital stage in their professional development. Survey (n = 124) and interview (n = 12) data were gathered from NQSWs across Scotland. Findings were considered through Giddens’ lens of ontological security, to explore NQSW transitions during a context of pandemic disruption and its impacts on NQSWs’ confidence and competence, as well as their sense of self and identity. Consistent with other studies, respondents were most impacted by home working and the associated isolation and separation from colleagues, particularly when engaged in emotionally charged work. Findings uncovered a trichotomy of experience, with variation in the quality and availability of some formal supports—induction, training and learning and development—and informal support. Implications for practice include a need to focus on how we support and nurture NQSWs at such a critical stage in their professional socialisation.
Velocity of conduction between columns and layers in barrel cortex reported by parvalbumin interneurons
Authors: Scheuer Katherine S , Judge John M , Zhao Xinyu , Jackson Meyer B 
Published: July 2023

Abstract
Inhibitory interneurons expressing parvalbumin (PV) play critical roles throughout the brain. Their rapid spiking enables them to control circuit dynamics on a millisecond time scale, and the timing of their activation by different excitatory pathways is critical to these functions. We used a genetically encoded hybrid voltage sensor to image PV interneuron voltage changes with sub-millisecond precision in primary somatosensory barrel cortex (BC) of adult mice. Electrical stimulation evoked depolarizations with a latency that increased with distance from the stimulating electrode, allowing us to determine conduction velocity. Spread of responses between cortical layers yielded an interlaminar conduction velocity and spread within layers yielded intralaminar conduction velocities in different layers. Velocities ranged from 74 to 473 μm/ms depending on trajectory; interlaminar conduction was 71% faster than intralaminar conduction. Thus, computations within columns are more rapid than between columns. The BC integrates thalamic and intracortical input for functions such as texture discrimination and sensory tuning. Timing differences between intra- and interlaminar PV interneuron activation could impact these functions. Imaging of voltage in PV interneurons reveals differences in signaling dynamics within cortical circuitry. This approach offers a unique opportunity to investigate conduction in populations of axons based on their targeting specificity.
EEG functional connectivity after perinatal stroke
Authors: Kavčič Alja , Demšar Jure , Georgiev Dejan , Meglič Nuška Pečarič , Šalamon Aneta Soltirovska 
Published: July 2023

Abstract
Impaired cognitive functioning after perinatal stroke has been associated with long-term functional brain network changes. We explored brain functional connectivity using a 64-channel resting-state electroencephalogram in 12 participants, aged 5–14 years with a history of unilateral perinatal arterial ischemic or haemorrhagic stroke. A control group of 16 neurologically healthy subjects was also included—each test subject was compared with multiple control subjects, matched by sex and age. Functional connectomes from the alpha frequency band were calculated for each subject and the differences in network graph metrics between the 2 groups were analyzed. Our results suggest that the functional brain networks of children with perinatal stroke show evidence of disruption even years after the insult and that the scale of changes appears to be influenced by the lesion volume. The networks remain more segregated and show a higher synchronization at both whole-brain and intrahemispheric level. Total interhemispheric strength was higher in children with perinatal stroke compared with healthy controls.
Association between dietary vitamin E intake and chronic kidney disease events in US adults: a cross-sectional study from NHANES 2009–2016
Authors: Li Jiyuan , Liu Ziyi , Pu Yan , Dai Helong , Peng Fenghua 
Published: July 2023

ABSTRACT

Background
The relationship between vitamin E supplementation and the prevalence of chronic kidney disease (CKD) is unclear. We discussed the relationship between vitamin E intake and CKD prevalence and further investigated the effect on different CKD risk strata.

Methods
We ultimately included 20 295 participants from the National Health and Nutrition Examination Survey (NHANES) database from 2009 to 2016. Multiple logistic regression and restricted cubic splines (RCS) were applied to explore the relationship between vitamin E intake and CKD prevalence and risk stratification. Subgroup analysis was applied to assess the stability of the association between vitamin E intake and CKD.

Results
In the CKD prevalence study, we found a negative association between high vitamin E intake and CKD prevalence through an adjusted multiple logistic regression model, the odds ratio (OR) was 0.86 [95% confidence interval (CI) 0.74–1.00; P for trend = .041] and RCS showed a nonlinear negative correlation (P-nonlinear = .0002, &lt;.05). In the CKD risk stratification study, we found that in very high–risk patients, the OR was 0.51 (95% CI 0.32–0.84; P for trend = .006) and the RCS also showed a nonlinear negative correlation (P-nonlinear &lt;.0001, &lt;.05). Subgroup analysis demonstrated that the correlations were stable across populations (P-values &gt;.01 for all interactions).

Conclusion
Dietary vitamin E intake was negatively associated with the prevalence of CKD in US adults. Increased vitamin E intake was a protective factor across CKD risk strata, and as vitamin E intake increased, there was a non-linear downward trend in the proportion progressing to very high–risk CKD.
Habitat and vertical stratification affect capture of stink bugs (Hemiptera: Pentatomidae) and biological control of the invasive brown marmorated stink bug
Authors: Cottrell Ted E , Tillman Glynn , Grabarczyk Erin E , Toews Michael , Sial Ashfaq , Lahiri Sriyanka 
Published: July 2023

Abstract
Spatiotemporal distribution of stink bugs (Hemiptera: Pentatomidae) and their natural enemies across farmscapes has been studied in more detail recently. However, the impact of plant height on vertical stratification of stink bugs and their natural enemies is rarely addressed across these diverse habitats. In this study, we examined capture of native stink bugs, the invasive brown marmorated stink bug, Halyomorpha halys (Stål)(Hemiptera:Pentatomidae), and a predaceous wasp, Astata occidentalis Cresson (Hymenoptera: Sphecidae) in pheromone-baited traps, in 2 habitats, woodlands of mostly deciduous trees mixed with some conifers and pecan orchards, in addition to their vertical stratification (0–13.7 m) at increasing heights. Furthermore, the effects of canopy height and habitat on H. halys egg mass predation and parasitism were evaluated. Adult H. halys were abundant in both habitats, but more nymphs were captured in pecan orchards. The same pattern was found for adult Euschistus servus (Say) (Hemiptera:Pentatomidae), Thyanta custator McAtee (Hemiptera:Pentatomidae), and A. occidentalis. In contrast, adult E. tristigmus (Say) (Hemiptera:Pentatomidae) and Chinavia hilaris (Say) (Hemiptera:Pentatomidae) were more abundant in woodlands. More nymphal H. halys and adults of E. servus, T. custator, and A. occidentalis were captured in ground traps than canopy traps in pecan. More adult and nymphal H. halys were captured at varying heights in the woodland canopy compared to near the ground as were adult E. tristigmus and C. hilaris. Both parasitism and predation occurred in woodland and pecan canopies. However, in one test we found that parasitism of H. halys egg masses occurred more often in the upper tree canopy, and more parasitism was detected in woodland than orchard habitat. In 2 tests, predation was higher in woodlands than pecan orchards. These results will aid in optimizing conservation biological control tactics in these habitats.
Changes in pulmonary function in lung cancer patients after segmentectomy or lobectomy: a retrospective, non-intervention, observation study
Authors: Bao Minwei , Lang Zhongping , Wang Zhuofu , Zhang Xuhong , Zhao Long 
Published: July 2023

Abstract

OBJECTIVES
Pulmonary segmentectomy (SE) became an increasingly popular method for resection of early-stage lung cancer. This study aims to compare the impact of single SE (SSE), multiple SE (MSE) and lobectomy (LE) on postoperative pulmonary function in patients with NSCLC.

METHODS
Medical records of a total of 1284 patients who underwent LE (n = 493), SSE (n = 558) and MSE (n = 233) at Shanghai Pulmonary Hospital from January 2013 to October, 2020 were retrospectively analysed. Pulmonary function tests (PFTs) were performed preoperatively and 12 months after surgery.

RESULTS
SSE was associated with a significantly smaller decline in the PFT values compared to MSE and LE. There was a poor consistency between the observed and expected (O/E) loss of pulmonary function in all study groups (P &lt; 0.05). Both LE and SE resulted in similar O/E ratios of all PFT parameters (P &gt; 0.05).

CONCLUSIONS
Overall loss of pulmonary function was much greater after LE than after both SSE and MSE. MSE was associated with higher postoperative pulmonary function decline compared to SSE but was still beneficial over LE. Both LE and SE groups had similar PFT loss per segment (P &gt; 0.05).
Diet, cardiovascular disease, and mortality in 80 countries
Authors: Mente Andrew , Dehghan Mahshid , Rangarajan Sumathy , O’Donnell Martin , Hu Weihong , Dagenais Gilles , Wielgosz Andreas , A. Lear Scott , Wei Li , Diaz Rafael , Avezum Alvaro , Lopez-Jaramillo Patricio , Lanas Fernando , Swaminathan Sumathi , Kaur Manmeet , Vijayakumar K , Mohan Viswanathan , Gupta Rajeev , Szuba Andrzej , Iqbal Romaina , Yusuf Rita , Mohammadifard Noushin , Khatib Rasha , Nasir Nafiza Mat , Karsidag Kubilay , Rosengren Annika , Yusufali Afzalhussein , Wentzel-Viljoen Edelweiss , Chifamba Jephat , Dans Antonio , Alhabib Khalid F , Yeates Karen , Teo Koon , Gerstein Hertzel C , Yusuf Salim 
Published: July 2023

Abstract

Aims
To develop a healthy diet score that is associated with health outcomes and is globally applicable using data from the Prospective Urban Rural Epidemiology (PURE) study and replicate it in five independent studies on a total of 245 000 people from 80 countries.

Methods and results
A healthy diet score was developed in 147 642 people from the general population, from 21 countries in the PURE study, and the consistency of the associations of the score with events was examined in five large independent studies from 70 countries. The healthy diet score was developed based on six foods each of which has been associated with a significantly lower risk of mortality [i.e. fruit, vegetables, nuts, legumes, fish, and dairy (mainly whole-fat); range of scores, 0–6]. The main outcome measures were all-cause mortality and major cardiovascular events [cardiovascular disease (CVD)]. During a median follow-up of 9.3 years in PURE, compared with a diet score of ≤1 points, a diet score of ≥5 points was associated with a lower risk of mortality [hazard ratio (HR) 0.70; 95% confidence interval (CI) 0.63–0.77)], CVD (HR 0.82; 0.75–0.91), myocardial infarction (HR 0.86; 0.75–0.99), and stroke (HR 0.81; 0.71–0.93). In three independent studies in vascular patients, similar results were found, with a higher diet score being associated with lower mortality (HR 0.73; 0.66–0.81), CVD (HR 0.79; 0.72–0.87), myocardial infarction (HR 0.85; 0.71–0.99), and a non-statistically significant lower risk of stroke (HR 0.87; 0.73–1.03). Additionally, in two case-control studies, a higher diet score was associated with lower first myocardial infarction [odds ratio (OR) 0.72; 0.65–0.80] and stroke (OR 0.57; 0.50–0.65). A higher diet score was associated with a significantly lower risk of death or CVD in regions with lower than with higher gross national incomes (P for heterogeneity &lt;0.0001). The PURE score showed slightly stronger associations with death or CVD than several other common diet scores (P &lt; 0.001 for each comparison).

Conclusion
A diet comprised of higher amounts of fruit, vegetables, nuts, legumes, fish, and whole-fat dairy is associated with lower CVD and mortality in all world regions, especially in countries with lower income where consumption of these foods is low.
Very-early symptomatic recurrence is associated with late recurrence after radiofrequency ablation of atrial fibrillation
Authors: Wang Zhen , Lai Yiwei , Wang Yufeng , Wang Jue , Jiang Chao , He Liu , Guo Xueyan , Li Songnan , Wang Wei , Jiang Chenxi , Liu Nian , Tang Ribo , Long Deyong , Sang Caihua , Du Xin , Dong Jianzeng , Ma Changsheng 
Published: July 2023

Abstract

Aims
After radiofrequency catheter ablation (RFCA) of atrial fibrillation (AF), the effect of very-early (within 48 h) symptomatic recurrence (VESR) on late (after 3 months of RFCA) recurrence (LR) has been seldomly reported. We aimed to explore the relationship between VESR and LR among post-RFCA patients.

Methods and results
This was a single-centre prospective cohort study that enrolled 6887 AF patients who received the first RFCA procedure from June 2018 to December 2021 at Beijing Anzhen Hospital. Patients were divided into four groups based on VESR and early (from 48 h to 3 months after RFCA) recurrence (ER): Group A (no VESR, no ER); Group B (VESR but no ER); Group C (ER but no VESR); and Group D (both VESR and ER). Three hundred and thirty (4.79%) patients experienced VESR (Groups B and D). With an average follow-up of 14.7 months after grouping, the Kaplan–Meier curve showed that LR risk in VESR patients was higher than in other patients (log-rank, P &lt; 0.001), and the difference was significant in both paroxysmal (log-rank, P &lt; 0.001) and persistent (log-rank, P &lt; 0.001) AF patients (P for interaction = 0.118). In multivariate analysis, Groups B, C, and D were associated with a 2.161-, 5.409-, and 7.401-fold increase in the risk of LR, respectively. What is more, compared with Group A, VESR-atrial tachycardia and VESR-AF were related to a 3.467- and 5.564-fold LR risk, respectively. In VESR patients, classification based on ER and VESR modes improved the prediction potential of LR risk.

Conclusion
Very-early symptomatic recurrence is associated with an increased risk of LR.
Cascading foreshocks, aftershocks and earthquake swarms in a discrete fault network
Authors: Im Kyungjae , Avouac Jean-Philippe 
Published: July 2023

SUMMARYEarthquakes come in clusters formed of mostly aftershock sequences, swarms and occasional foreshock sequences. This clustering is thought to result either from stress transfer among faults, a process referred to as cascading, or from transient loading by aseismic slip (pre-slip, afterslip or slow slip events). The ETAS statistical model is often used to quantify the fraction of clustering due to stress transfer and to assess the eventual need for aseismic slip to explain foreshocks or swarms. Another popular model of clustering relies on the earthquake nucleation model derived from experimental rate-and-state friction. According to this model, earthquakes cluster because they are time-advanced by the stress change imparted by the mainshock. This model ignores stress interactions among aftershocks and cannot explain foreshocks or swarms in the absence of transient loading. Here, we analyse foreshock, swarm and aftershock sequences resulting from cascades in a Discrete Fault Network model governed by rate-and-state friction. We show that the model produces realistic swarms, foreshocks and aftershocks. The Omori law, characterizing the temporal decay of aftershocks, emerges in all simulations independently of the assumed initial condition. In our simulations, the Omori law results from the earthquake nucleation process due to rate and state friction and from the heterogeneous stress changes due to the coseismic stress transfers. By contrast, the inverse Omori law, which characterizes the accelerating rate of foreshocks, emerges only in the simulations with a dense enough fault system. A high-density complex fault zone favours fault interactions and the emergence of an accelerating sequence of foreshocks. Seismicity catalogues generated with our discrete fault network model can generally be fitted with the ETAS model but with some material differences. In the discrete fault network simulations, fault interactions are weaker in aftershock sequences because they occur in a broader zone of lower fault density and because of the depletion of critically stressed faults. The productivity of the cascading process is, therefore, significantly higher in foreshocks than in aftershocks if fault zone complexity is high. This effect is not captured by the ETAS model of fault interactions. It follows that a foreshock acceleration stronger than expected from ETAS statistics does not necessarily require aseismic slip preceding the mainshock (pre-slip). It can be a manifestation of a cascading process enhanced by the topological properties of the fault network. Similarly, earthquake swarms might not always imply transient loading by aseismic slip, as they can emerge from stress interactions.
Phyllosphere bacterial strains Rhizobium b1 and Bacillus subtilis b2 control tomato leaf diseases caused by Pseudomonas syringae pv. tomato and Alternaria solani
Authors: Shao Ziyu , Schenk Peer M , Dart Peter 
Published: July 2023

Abstract

Aims
Show that tomato leaf phyllosphere bacteria are candidates for biocontrol of tomato leaf diseases.

Methods and Results
Seven bacterial isolates from surface-sterilized Moneymaker tomato plants were tested for growth inhibition of 14 tomato pathogens on potato dextrose agar. Biocontrol assays were conducted with tomato leaf pathogens, Pseudomonas syringae pv. tomato (Pto) and Alternaria solani (A. solani). Two potential isolates showing the greatest inhibition were identified by 16S rDNA sequencing as Rhizobium sp. (isolate b1) and Bacillus subtilis (isolate b2), both produce protease and isolate b2 cellulase. Both reduced tomato leaf infections by Pto and A. solani in detached leaf bioassays. Both bacteria b1 and b2 reduced pathogen development in a tomato growth trial. Bacteria b2 also induced the tomato plant salicylic acid (SA) immune response pathway. Disease suppression in biocontrol assays with b1 and b2 varied between five commercial tomato varieties.

Conclusions
Tomato phyllosphere bacteria when used as phyllosphere inoculants, inhibited tomato diseases caused by Pto and A. solani.
The NYU Data Catalog: a modular, flexible infrastructure for data discovery
Authors: Yee Michelle , Surkis Alisa , Lamb Ian , Contaxis Nicole 
Published: July 2023

Abstract

Objective
Researchers at New York University (NYU) Grossman School of Medicine contacted the Health Sciences Library for help with locating large datasets for reuse. In response, the library developed and maintained the NYU Data Catalog, a public-facing data catalog that has supported not only faculty acquisition of data but also the dissemination of the products of their research in various ways.

Materials and Methods
The current NYU Data Catalog is built upon the Symfony framework with a tailored metadata schema reflecting the scope of faculty research areas. The project team curates new resources, including datasets and supporting software code, and conducts quarterly and annual evaluations to assess user interactions with the NYU Data Catalog and opportunities for growth.

Results
Since its launch in 2015, the NYU Data Catalog underwent a number of changes prompted by an increase in the disciplines represented by faculty contributors. The catalog has also utilized faculty feedback to enhance support of data reuse and researcher collaboration through alterations to its schema, layout, and visibility of records.

Discussion
These findings demonstrate the flexibility of data catalogs as a platform for enabling the discovery of disparate sources of data. While not a repository, the NYU Data Catalog is well-positioned to support mandates for data sharing from study sponsors and publishers.

Conclusion
The NYU Data Catalog makes the most of the data that researchers share and can be harnessed as a modular and adaptable platform to promote data sharing as a cultural practice.