bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real‐time liver motion estimation via deep learning‐based angle‐agnostic X‐ray imaging
Authors: Shao Hua‐Chieh , Li Yunxiang , Wang Jing , Jiang Steve , Zhang You 
Published: September 2023

AbstractBackgroundReal‐time liver imaging is challenged by the short imaging time (within hundreds of milliseconds) to meet the temporal constraint posted by rapid patient breathing, resulting in extreme under‐sampling for desired 3D imaging. Deep learning (DL)‐based real‐time imaging/motion estimation techniques are emerging as promising solutions, which can use a single X‐ray projection to estimate 3D moving liver volumes by solved deformable motion. However, such techniques were mostly developed for a specific, fixed X‐ray projection angle, thereby impractical to verify and guide arc‐based radiotherapy with continuous gantry rotation.PurposeTo enable deformable motion estimation and 3D liver imaging from individual X‐ray projections acquired at arbitrary X‐ray scan angles, and to further improve the accuracy of single X‐ray‐driven motion estimation.MethodsWe developed a DL‐based method, X360, to estimate the deformable motion of the liver boundary using an X‐ray projection acquired at an arbitrary gantry angle (angle‐agnostic). X360 incorporated patient‐specific prior information from planning 4D‐CTs to address the under‐sampling issue, and adopted a deformation‐driven approach to deform a prior liver surface mesh to new meshes that reflect real‐time motion. The liver mesh motion is solved via motion‐related image features encoded in the arbitrary‐angle X‐ray projection, and through a sequential combination of rigid and deformable registration modules. To achieve the angle agnosticism, a geometry‐informed X‐ray feature pooling layer was developed to allow X360 to extract angle‐dependent image features for motion estimation. As a liver boundary motion solver, X360 was also combined with priorly‐developed, DL‐based optical surface imaging and biomechanical modeling techniques for intra‐liver motion estimation and tumor localization.ResultsWith geometry‐aware feature pooling, X360 can solve the liver boundary motion from an arbitrary‐angle X‐ray projection. Evaluated on a set of 10 liver patient cases, the mean (± s.d.) 95‐percentile Hausdorff distance between the solved liver boundary and the “ground‐truth” decreased from 10.9 (±4.5) mm (before motion estimation) to 5.5 (±1.9) mm (X360). When X360 was further integrated with surface imaging and biomechanical modeling for liver tumor localization, the mean (± s.d.) center‐of‐mass localization error of the liver tumors decreased from 9.4 (± 5.1) mm to 2.2 (± 1.7) mm.ConclusionX360 can achieve fast and robust liver boundary motion estimation from arbitrary‐angle X‐ray projections for real‐time imaging guidance. Serving as a surface motion solver, X360 can be integrated into a combined framework to achieve accurate, real‐time, and marker‐less liver tumor localization.
MAPping affective dimensions of behavior: Methodologic and pragmatic advancement of the Multidimensional Assessment Profiles scales
Authors: Wiggins Jillian Lee , Roy Amy K. , Wakschlag Lauren S. 
Published: September 2023

AbstractRigorous validation of the full developmentally sensitive normal:abnormal spectrum, including evaluating the incremental value of age‐specific behaviors, is necessary for nuanced characterization of dimensional features of psychopathology. To maximize the clinical utility of transdiagnostic approaches to risk identification, derivation of psychometrically sound, pragmatic versions with empirically derived cutoffs is also key. This special section has a central focus on rigorous, developmentally‐based measurement of irritability as an exemplar of this theory‐ and pragmatically‐based approach. Elevated irritability is a robust transdiagnostic predictor of the common psychopathologies of childhood. The Multidimensional Assessment Profiles Temper Loss (MAPS‐TL) Scales are the only irritability tool specifically designed to capture the normal:abnormal dimensional spectrum. These have been extensively investigated in preschool age but lack rigorous modeling at older and younger ages. In this special issue, (with three independent—and one longitudinal—set of samples), we test and improve measurement of irritability as a transdiagnostic phenotype of psychopathology risk as it unfolds across development, expanding the MAPS‐TL scale in three important ways: (1) extending irritability dimensional modeling and the developmental specification approach to older ages, (2) advancing science to practice translation by generating pragmatic irritability screening tools across ages, and (3) extending the dimensional, developmental specification approach to other dimensions of behavior, that is, internalizing. Collectively, the special issue operationalizes and advances application of a neurodevelopmental, dimensional and transdiagnostic approach to psychopathology.
Self‐reported barriers in self‐management of women with gestational diabetes: A systematic review of qualitative studies
Authors: Xu Nuo , Han Xia , Chen Sijing , Zhang Jingjing , Gu Ping 
Published: September 2023

AbstractAimTo provide a systematic review of the qualitative literature on self‐reported barriers to self‐management in pregnant women with gestational diabetes mellitus (GDM).DesignSystematic review.MethodsThis systematic review followed the Joanna Briggs Institute meta‐aggregation approach and was evaluated using the Joanna Briggs Institute Critical Appraisal Checklist for Qualitative Research. Qualitative studies on the barriers to self‐management among GDM pregnancy survivors conducted until 17 May 2022, were searched.ResultsA total of 30 studies were included, of which seven were in Chinese and 23 were in English, and 10 findings resulted in three themes: (a) Knowledge and belief, (b) Skills and abilities and (c) Environment and social support. By summarizing the self‐reported barriers to self‐management in patients with GDM and recommends precise interventions for these barriers, thereby saving health resources and helping to increase their willingness and ability to engage in self‐management.
Radially Pedicled In‐Situ Split‐Thickness Skin Grafts, an Alternative to Distal Split‐Thickness Skin Grafts
Authors: Zhang Lei , Zhou Xu‐Lin , Shi Cheng‐Ji , Fu Guang‐Xin , Zhao Dan 
Published: September 2023

AbstractObjectiveThis study aimed to introduce a novel radially pedicled in‐situ split‐thickness skin graft (STSG). The morbidity, esthetic, and functional outcomes of the radially pedicled in‐situ STSG were in comparison with those of the distal STSG.Study DesignRetrospective analysis.SettingA single‐institution review.MethodsSeventy patients with oral cancer who underwent radical surgical resection and simultaneous radial forearm free flap (RFFF) reconstruction from July 2021 to March 2022 were included. De‐epithelialized RFFFs and traditional RFFFs were used to repair oral defects of 35 patients in Group A and Group B, respectively, while radially pedicled in‐situ STSGs and distal STSGs taken from abdomens were used to repair donor site defects in the above groups, respectively. Patient demographics, wound healing complications, and esthetic and functional outcomes of the forearms were compared between the 2 groups.ResultsNo significant difference between Group A and Group B was observed in terms of donor site and recipient site complications. The esthetic outcome was superior in Group A compared to Group B (P = .011). The extension range, sensation, and pinch strength of operated forearms were significantly reduced in both groups after surgery (P < .05), however, intergroup differences were not observed.ConclusionTaken together, our results suggest that radially pedicled in‐situ STSG is an applicable technique for direct closure and better esthetic outcomes in the forearm donor site.
Algebraic properties of projected problems in LSQR
Authors: Havelková Eva , Hnětynková Iveta 
Published: September 2023

AbstractLSQR represents a standard Krylov projection method for the solution of systems of linear algebraic equations, linear approximation problems or regularization of discrete inverse problem. Its convergence properties (residual norms, error norms, influence of finite precision arithmetic etc.) have been widely studied. It has been observed that the components of the solution of the projected bidiagonal problem typically increase and their sign alternates. This behavior is the core of approximation properties of LSQR and is observed also for hybrid LSQR with inner Tikhonov regularization. Here we provide rigorous analysis of sign changes and monotonicity of individual components of projected solutions and projected residuals in LSQR. The results hold also for Hybrid LSQR with a fixed inner regularization parameter. The derivations do not rely on maintaining orthogonality in Krylov bases determined by the bidiagonalization process. Numerical illustration is included.
An arbitrary Lagrangian–Eulerian formulation for the Reynolds equation considering the JFO boundary condition
Authors: Tong Yan , Müller Michael , Ostermeyer Georg‐Peter 
Published: September 2023

AbstractIn the realm of lubrication theory, the hydrodynamics of the fluid lubricant are commonly elucidated through the Reynolds equation. Its establishment stems from using the Eulerian description method, restricting the fluid domain to a fixed spatial configuration. However, certain scenarios necessitate coupling the Reynolds equation with solid deformation, wherein the fluid boundary undergoes alterations concurrent with the displacement or deformation of the solid. In such cases, the employment of a movable fluid domain becomes imperative. To address this requirement, this contribution introduces the arbitrary Lagrangian–Eulerian method, which effectively transforms the reference system of the Reynolds equation into a fluid domain amenable to arbitrary movement. Subsequently, the weak formulation associated with the movable fluid domain is derived and synergistically combined with a preexisting cavitation formulation. This integration facilitates an efficient solution of the Reynolds equation utilizing the finite element method while duly accounting for the mass‐conserving cavitation effects. Ultimately, a transient hydrodynamic lubrication example is presented to demonstrate the feasibility of the newly developed formulations.
Cellulose nanofiber‐incorporated high‐solid siloxane acrylic latex by mini‐emulsion polymerization for hydrophobic coating and wood adhesive
Authors: Neelambaram Punugupati , Thounchiyath Thushara , Narayan Ramanuj , Chakrabarty Arindam 
Published: September 2023

AbstractIn order to contribute to the thriving research on bio nanocomposites for sustainable future, here we developed a cellulose nanofiber (CNF)‐based siloxane acrylic nanocomposite aiming to the application as hydrophobic coating and wood adhesive. CNF was incorporated in a copolymer matrix of poly (methyl methacrylate‐co‐butyl acrylate) [P(MMA‐co‐BA)] cross‐linked with methacrylate‐terminated siloxane (MTS). The copolymer was prepared by mini‐emulsion polymerization at a high solid content of about 57 wt%, where CNF was incorporated to the latex both by in‐situ polymerization and ex‐situ mixing process. The impact of the process of CNF incorporation and its content in the copolymer matrix were evaluated by measuring the thermal, mechanical, water repelling and adhesion properties. All the nanocomposites showed improved mechanical properties (tensile strength: upto 3.9 MPa) and wood adhesion strength (upto 1178 N) compared to the pristine copolymer. In addition, they showed significantly high water‐repelling properties on spray‐coated cotton (water contact angle: 110°–124°). Such siloxane acrylic/CNF nanocomposite has the potential as a sustainable material in the application area of hydrophobic coatings and adhesives.
Mechanical behavior of three‐harness twill woven composite plates under tension loading
Authors: Lu Konghan , Cui Haitao , Zhou Yu , Chang Yipeng , Wang Nan , Zhang Hongjian 
Published: September 2023

AbstractThree‐harness twill woven composite (THT‐WC) is an advanced carbon fiber reinforced composite that compromises the mechanical behaviors in both longitudinal and latitudinal directions. To explore the further practical application, the structural characteristics and static mechanical behavior of the material were investigated in this article. X‐ray computerized tomography was adopted to obtain the mesostructure details and to capture the key characteristics. With the help of mesoscale information, the 2D and 3D geometric models were established in the form of representative volume elements (RVEs). Combined with voxel mesh generation technology, a finite element model was proposed based on a 3D geometric model where macroscopic mechanical properties of materials along different yarn directions were gained. To verify the simulated geometry and predicted mechanical properties, mesoscale geometric comparison and specimen tensile tests were performed. The results show that the geometric model established in this article can reasonably reflect the periodicity fluctuation of binder warp yarns and the randomness fluctuation of weft yarns. Compared with the experimental results, the relative error of stiffness in the weft direction of the RVE is 4.25%, and that in the warp direction is 5.33%.Highlights
The mechanical properties of THT‐WC were investigated experimentally.
Meso‐scale reconstruction of THT‐WC was presented.
A model to predict the mechanical properties of THT‐WC is established.
Music performance anxiety in children 9−12 years old in a music program
Authors: Tardif Catherine , Boucher Hélène , Lane Julie , Barbeau Audrey‐Kristel 
Published: September 2023

AbstractMusic‐intensive school program are one way of enhancing arts‐education in public schools. Elementary school students enrolled in a music‐intensive program are exposed to several stress factors that could contribute to development of music performance anxiety (MPA). This study aims to describe MPA manifestations as reported by 164 students from 9 to 12 years of age taking part in a music‐intensive school program, and to identify what may contribute to its development. Data were collected using a sociodemographic questionnaire, and the French version of the Music Performance Anxiety Inventory for Adolescents. Although it was expected that being involved in an intensive music program would bring students to report higher levels of MPA, results showed moderate levels of MPA. In addition, girls reported significatively more manifestations of MPA than boys. The MPA level also vary according to the origin of the registration in the program (child's request or parents' suggestion), and the cycle. Indeed, cycle 2 students reported significantly less MPA than cycle 3 students when the request to participate in the music‐intensive program came from the child. Findings provide useful key points that should be considered for both practitioners and researchers.
Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity
Authors: Krishnan Anagha , Cano Melissa , Karns Devin A. , Burch Tyson A. , Likhogrud Maria , Aqui Moena , Bailey Shaun , Verruto John , Lambert William , Kuzminov Fedor , Naghipor Mahva , Wang Yingjun , Ebmeier Christopher C. , Weissman Joseph C. , Posewitz Matthew C. 
Published: September 2023

AbstractHigh cellular pigment levels in dense microalgal cultures contribute to excess light absorption. To improve photosynthetic yields in the marine microalga Picochlorum celeri, CAS9 gene editing was used to target the molecular chaperone cpSRP43. Depigmented strains (>50% lower chlorophyll) were generated, with proteomics showing attenuated levels of most light harvesting complex (LHC) proteins. Gene editing generated two types of cpSRP43 transformants with distinct lower pigment phenotypes: (i) a transformant (Δsrp43) with both cpSRP43 diploid alleles modified to encode non‐functional polypeptides and (ii) a transformant (STR30309) with a 3 nt in‐frame insertion in one allele at the CAS9 cut site (non‐functional second allele), leading to expression of a modified cpSRP43. STR30309 has more chlorophyll than Δsrp43 but substantially less than wild type. To further decrease light absorption by photosystem I in STR30309, CAS9 editing was used to stack in disruptions of both LHCA6 and LHCA7 to generate STR30843, which has higher (5–24%) productivities relative to wild type in solar‐simulating bioreactors. Maximal productivities required frequent partial harvests throughout the day. For STR30843, exemplary diel bioreactor yields of ~50 g m−2 day−1 were attained. Our results demonstrate diel productivity gains in P. celeri by lowering pigment levels.
Microbiological patterns of bacterial infections in tracheostomized children: Reducing uncertainty in continuous care
Authors: García‐Boyano Miguel , Alcalá Francisco José Climent , Alonso Aroa Rodríguez , de Villalta Marta García Fernández , Alonso Oihane Zubiaur , Retolaza Ignacio Rabanal , Melero Inmaculada Quiles , Calvo Cristina , García Luis Escosa 
Published: September 2023

AbstractBackground and ObjectivesRespiratory infections are the most frequent cause of hospitalization in tracheostomized children. However, there is a lack of publications to guide their management. The primary objective was to describe the microbiological isolates and their antibiotic susceptibilities of bacterial respiratory infections in a population of tracheostomized children.MethodsRetrospective follow‐up study of children with tracheostomy seen at a tertiary hospital. Respiratory infection episodes in patients under 18 years of age who had a tracheostomy and bacterial isolation with a count ≥104 Colony Forming Units/mL in tracheal aspirate were included.ResultsThe study analyzed 328 respiratory infection episodes: 164 tracheobronchitis (50%), 112 nonspecific respiratory episodes (34.1%), and 52 pneumonias (15.9%). The most commonly isolated microorganisms were Pseudomonas aeruginosa, Serratia marcescens, and Staphylococcus aureus. The antibiotics that exhibited the highest effectiveness were meropenem (92%), imipenem (87%), and levofloxacin (86%). When hospitalization exceeded 7 days, there was a higher chance of isolating Escherichia coli and Klebsiella pneumoniae (p < 0.001 and p = 0.001, respectively), as well as an increased rate of multidrug resistance (27% vs. 7%, p = 0.035). In 75.3% of cases, the microorganism had been previously isolated in a sample taken 7−30 days before the current one, with a higher frequency observed in the case of P. aeruginosa (95.2%) compared to other microorganisms (65.3%, p < 0.001).ConclusionsMeropenem, imipenem, and levofloxacin provided the most effective coverage for these infections. The risk of multidrug resistance increased with longer hospital stays, especially for E. coli and K. pneumoniae. Recent isolation of P. aeruginosa may justify empirical coverage.
Amber‐codon suppression for spatial localization and in vivo photoaffinity capture of the interactome of the Pseudomonas aeruginosa rare lipoprotein A lytic transglycosylase
Authors: Avila‐Cobian Luis F. , Hoshino Hidekazu , Horsman Mark E. , Nguyen Van T. , Qian Yuanyuan , Feltzer Rhona , Kim Choon , Hu Daniel D. , Champion Matthew M. , Fisher Jed F. , Mobashery Shahriar 
Published: September 2023

AbstractThe 11 lytic transglycosylases of Pseudomonas aeruginosa have overlapping activities in the turnover of the cell‐wall peptidoglycan. Rare lipoprotein A (RlpA) is distinct among the 11 by its use of only peptidoglycan lacking peptide stems. The spatial localization of RlpA and its interactome within P. aeruginosa are unknown. We employed suppression of introduced amber codons at sites in the rlpA gene for the introduction of the unnatural‐amino‐acids Νζ‐[(2‐azidoethoxy)carbonyl]‐l‐lysine (compound 1) and Nζ‐[[[3‐(3‐methyl‐3H‐diazirin‐3‐yl)propyl]amino]carbonyl]‐l‐lysine (compound 2). In live P. aeruginosa, full‐length RlpA incorporating compound 1 into its sequence was fluorescently tagged using strained‐promoted alkyne‐azide cycloaddition and examined by fluorescence microscopy. RlpA is present at low levels along the sidewall length of the bacterium, and at higher levels at the nascent septa of replicating bacteria. In intact P. aeruginosa, UV photolysis of full‐length RlpA having compound 2 within its sequence generated a transient reactive carbene, which engaged in photoaffinity capture of neighboring proteins. Thirteen proteins were identified. Three of these proteins—PBP1a, PBP5, and MreB—are members of the bacterial divisome. The use of the complementary methodologies of non‐canonical amino‐acid incorporation, photoaffinity proximity analysis, and fluorescent microscopy confirm a dominant septal location for the RlpA enzyme of P. aeruginosa, as a divisome‐associated activity. This accomplishment adds to the emerging recognition of the value of these methodologies for identification of the intracellular localization of bacterial proteins.
Rate limiting step of the allosteric activation of the bacterial adhesin FimH investigated by molecular dynamics simulations
Authors: Interlandi Gianluca 
Published: September 2023

AbstractThe bacterial adhesin FimH is a model for the study of protein allostery because its structure has been resolved in multiple configurations, including the active and the inactive state. FimH consists of a pilin domain (PD) that anchors it to the rest of the fimbria and an allosterically regulated lectin domain (LD) that binds mannose on the surface of infected cells. Under normal conditions, the two domains are docked to each other and LD binds mannose weakly. However, in the presence of tensile force generated by shear the domains separate and conformational changes propagate across LD resulting in a stronger bond to mannose. Recently, the crystallographic structure of a variant of FimH has been resolved, called , where PD contains 10 mutations near the inter‐domain interface. Although the X‐ray structures of FimH and are almost identical, experimental evidence shows that is activated even in the absence of shear. Here, molecular dynamics simulations combined with the Jarzynski equality were used to investigate the discrepancy between the crystallographic structures and the functional assays. The results indicate that the free energy barrier of the unbinding process between LD and PD is drastically reduced in . Rupture of inter‐domain hydrogen bonds involving R166 constitutes a rate limiting step of the domain separation process and occurs more readily in than FimH. In conclusion, the mutations in shift the equilibrium toward an equal occupancy of bound and unbound states for LD and PD by reducing a rate limiting step.