bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strategies for Indonesia’s Agricultural Climate Change Adaptation and Mitigation
Authors: Agus Fahmuddin , Surmaini Elza , Lina Helena 
Published: February 2023

<p>Agriculture is known as one of the sectors that both contributes to and is the victim of climate change. Under the adverse impacts of climate change, producing more food is an advance challenge. Rainfall and temperature controls crop growing seasons. The frequency and intensity of the occurrence of floods and droughts are increasing, which causes crop yield decline. On the other hand, climate change is also affected by agriculture sector’s emissions. Agriculture in Indonesia emitted about 104 Mt (million tonnes) CO<sub>2</sub>e in 2020 with the main sources of emission were from lowland rice, livestock enteric fermentation, direct N<sub>2</sub>O from soil and manure and indirect N<sub>2</sub>O from soil. Emission reduction strategies in Indonesia’s Nationally Determined Contribution (NDC) include the use of low-emission lowland rice crops varieties, implementation of efficient water management in lowland rice cultivation, manure management for biogas, and feed quality improvement for livestock. Moreover, for reaching the national emission reduction target some additional actions are needed, including: intensified use of organic fertilizers, organic village, balance fertilization which is mostly associated with improving N use efficiency, and raising water in peatland farming. At the national level, climate change policy is more focused on mitigation ambition above that of NDC. Therefore, the country needs to look for ways that combine climate change mitigation and ensuring food security. All policies of mitigation must be in synchrony with adaptation since adaptation is the key for successful maintenance of food security. <strong></strong></p>
A bibliometric and visualization analysis of the aerosol research on glaciers in the Indian Himalayan Region (IHR)
Authors: Pippal Prity S , Kumar Ramesh , Singh Atar , Kumar Rajesh 
Published: February 2023

Abstract

This research focuses on a bibliometric analysis of research on aerosols' impact on the glaciers in the Indian Himalayan region (IHR) published in journals from all subject categories based on the Science Citation Index Expanded, collected from the Web of Science and Scopus database between the years 2002 and April 2022. The indexing phrases like "aerosol," "glacier," and "snow" are commonly used terms and have been utilized to collect the related publications for this investigation. The document selections were based on years of publication, authorship, the scientific output of authors, distribution of publication by country, categories of the subjects, and names of journals in which scholarly papers were published. The journal's impact factor trend, citation trend, and the count of keywords used by authors were also included. The number of articles on aerosols accelerating the melting of glaciers shows a notable increase in recent years, along with more glacier melting results from countries involved in climate science research. China (382) was the country with the highest publication output on aerosols accelerating the melting of glaciers. The United States (367) was the most cited country with about 17500 total citations and 80.40 average citations per year from 2002-April 2022. The results reveal that research trends in the glaciers on aerosols' impact on the glaciers have been attractive in recent years, and the number of articles in this field keeps increasing fast.
MST2 Acts via AKT Activity to Promote Neuronal Axon Regeneration and Functional Recovery after Spinal Cord Injury in Mice
Authors: Zheng Hongming , Wang Honghai , Xu Yi , Xu Xu , Zhu Zhenghuan , Fang Jiawei , Song Zhiwen , Liu Jinbo 
Published: February 2023

Abstract

Background​Mammalian sterile 20-like kinase 2 (MST2), a core component of the Hippo signaling pathway, plays an crucial role in apoptosis and cell growth. However, its role in axon regeneration after spinal cord injury (SCI) was first elucidated in this study.Methods​We first screened the proteins involved in spinal cord contusion injury using a mouse model utilizing 4D label-free proteomic analysis. We selected the neuronal axon regeneration-associated protein MST2 as our target protein. In vitro and in vivo experiments were applied to explore the function of MST2. We then performed additional experiments, such as co-immunoprecipitation and so on to confrm the downstream molecular mechanisms of MST2.Results​We confirmed that MST2, mainly expressed in neurons, promoted axon regeneration by positively regulating AKT activity in neuronal cell models and primary cortical neurons. Mechanistically, our studies found that MST2 protein could interact both with AKT and p-AKT in vitro. In vivo, MST2 knockdown inhibits axon regeneration and motor functional recovery, while lentiviral-mediated overexpression of MST2 promoted axon regeneration and motor functional recovery after SCI. However, MST2 induced axon regeneration and motor functional recovery were reversed by AKT inhibitors.Conclusion​This study provided evidence that how MST2 acts as a new regulator that controls axon regeneration after SCI, MST2 promotes neuronal axon regeneration by positively regulating AKT activity, indicating MST2 may be a target with great therapeutic potential for SCI.
Moderate Biochar Application and Increases in Lignin and Silicon Concentrations in Stalks Synergistically Enhance Lodging Resistance of Rice
Authors: Gong Diankai , Yu Guangxing 
Published: February 2023

Abstract
To clarify the effects of biochar on measuring the lodging-related traits, structural carbohydrates, and the activity and gene expression of enzymes related to lignin synthesis in rice stalks, to screen the optimal dosage of biochar and to investigate the mechanism of biochar in regulating the lodging-resistant ability of rice stalks. SN265 and Akihikari, two rice varieties with evident differences in lodging resistance, were selected and treated with biochar at five dosages: 0,5,10,20,40t/hm2. With increase of the dosage of biochar, The expression levels of COMT, 4CL3, CCR20 and PAL genes related to lignin synthesis were the highest under B3 treatment. The silicon concentration in the two varieties under B3, B4 and B5 treatments was notably higher than that under B1 treatment. The numbers of large and small vascular bundles were the largest in SN265 under B3 treatment and in Akihikari under B2 treatment. Moreover, the two varieties exhibited the highest lignin concentration, the smallest lodging index and the strongest lodging resistance under B3 treatment. A certain amount of biochar can regulate the corresponding enzyme activity by increasing the expression level of lignin synthesis-related genes, and evidently increase the lignin concentration in stalks. The application of rice hull charcoal is able to dramatically raise the silicon concentration in stalks, thus improving the lodging resistance of stalks. The two approaches can synergistically improve the internode traits and breaking strength and strengthen the lodging resistance of stalks. In this study, 5–10 t/hm2 is the appropriate dosage in treatment.
Effect of bias conditions on transient anode current for quasi-double-sided silicon drift detector with high energy resolution
Authors: wang longjie , Luo Wei , Zhang Ming peng , Jia Rui , Li Xing , Tian Xiao-Rang , Wang Bolong , Jiang Shuai , Cheng Jia-Wang , Ouyang Xiao-Ping 
Published: February 2023

Abstract

Silicon drift detectors (SDDs) are widely applied for x-ray detection due to their remarkable energy resolution. The main contribution to energy resolution is the electrical noise of measurement system. In addition, other factors, including imperfect charge collection and ballistic deficit, can deteriorate the energy resolution. Those factors are closely related to the bias conditions of the device. However, the effect of bias conditions on device energy resolution has rarely been reported, primarily because the applied bias of classical SDDs is limited by twice the depletion voltage and little attention is focused on bias conditions. In order to enhance the flexibility of bias conditions and investigate the effect of bias conditions on energy resolution, we proposed a novel quasi-double-sided silicon drift detector (QD-SDD) with a drift ring structure designed on the back side. Device simulations of SDD and QD-SDD under different bias conditions were performed to obtain the transient current response of the anode. It was found that the QD-SDD has greater flexibility in bias conditions, and then better electron collection efficiency and shorter electron collection time can be realized at higher bias voltage (> 200 V). Finally, the fabricated QD-SDD was characterized using a 55Fe radioactive source. The experiment results showed that the energy resolution of QD-SDD varies with different bias conditions. The optimal energy resolution is 170 eV under the optimized bias conditions.
May the fibrinogen to albumin ratio be a prognostic factor in acute mesenteric ischemia? Is it worth mentioning?
Authors: MUHTAROĞLU ALİ , ÇAPOĞLU Recayi , UYGUR Furkan Ali , HARMANTEPE Ahmet Tarık , BAYHAN Zülfü , GÖNÜLLÜ Emre 
Published: February 2023

Abstract

Objective:Acute mesenteric ischemia (AMI) is a vascular emergency resulting from decreased blood flow caused by the occlusion of the mesenteric vessels, hypoperfusion or vasospasm. This study aimed to investigate the prognostic value of the fibrinogen-to-albumin (FAR) ratio in patients with acute mesenteric ischemia.Methods:A total of 91 patients were enrolled in the study. Patients' demographics such as age and gender, pre- and postoperative hemoglobin, CRP, white blood cell (WBC), neutrophils, preoperative lymphocyte, alanine transaminase (ALT), aspartate transaminase (AST), thrombocytes and postoperative D-dimer values were recorded. In addition, pre- and postoperative fibrinogen and albumin levels were recorded and FAR was calculated. Patients were divided into two groups survivors and non-survivors.Results:The mean pre- and postoperative fibrinogen levels were statistically significantly higher in the non-survivor group than in the survivor group (p<0.001). The mean pre- and postoperative albumin levels were significantly lower in the non-survivors than in the survivors (p=0.059, p<0.001; respectively). The mean pre- and postoperative FAR ratios were considerably higher in the non-survivor than in the survivor groups (p<0.001). The change between pre- and postoperative fibrinogen, albumin and FAR values were statistically significant between the non-survivors and the survivors (for all, p<0.05).Conclusion:The preoperative and postoperative fibrinogen levels were significantly higher, and albumin levels were significantly lower in the survivor compared to the non-survivor patients with AMI. Furthermore, the preoperative and postoperative FAR ratio was significantly higher in the non-survivors. The FAR ratio may be a valuable prognostic biomarker for patients with AMI.
Tailoring Electrical Properties of SWCNT’s by Deformation Method
Authors: Lamba Vijay Kumar , Kassa Sankit , Brar Loveleen 
Published: February 2023

Abstract
For more efficient energy distribution system, we need a high efficiency material for charge transport. In present work we combined continuum mechanics methods for studying the effect of strain on CNT, and the change in electrical parameters as a result of CNT deformation using first principal method. We found that on applying stress on SWCNT’s, the bond lengths increase near the point of applied force from 1.42 to 1.88Å (just below the applied force and on opposite face it changes from 1.42 to 2.24 Å), and at the edged region, the bond length decreases from 1.42 to 1.28 on both side. Further there is decrease in trans conductance in A (6,6) and A(8,8), due to induces gap opening i.e. splitting of band in high energy region
Coding DNA of the recombinant panfilovirus mAb 7C8F1: cloning and expression in CHO mammalian cell lines
Authors: Vlachakis Dimitrios , Plewczynski Dariusz , Bongcam-Rudloff Erik , Jovelin Fabien , Weyer Jacqueline , Paweska Janusz T , WAYENGERA Misaki 
Published: February 2023

Abstract
Background: Uganda recently experienced an outbreak of sudan ebolavirus (SUDV) species, for which there are no approved or licensed rapid diagnostic tests (RDTs). Our group has a longstanding history in the research and development (R & D) of panfilovirus RDTs. This paper describes sequencing of the coding DNA of a recombinant monoclonal antibody (7C8F1) targeting the epitope (YEAGEWAENCY, dubbed peptide 2) conserved across all ebolavirus spp glycoproteins (GP).
Methods: Mice hybridoma were prepared & cultured on Dulbecco’s modified eagle’s medium (DMEM) Total RNA was extracted using RNA-easy isolation reagent (Vazyme), and reverse transcribed into cDNA using SMARTScribe reverse transcriptase. Antibody fragments of heavy (H) and light (L) chains were amplified by rapid amplification of cDNA ends (RACE) and cloned into standard vectors for screening in 5 colonies. Inserts of matching phenotypic colonies were sequenced by Sanger capillary sequencing and aligned by IMGT analysis of VDJ. Isotyping was done by constant region analysis.
Results: Consensus sequences of the coding DNA of the heavy and light chains of an isotype IgG1/kappa recombinant mice mAb (Genbank accession #s OP966768 and OP966769, respectively) are presented. The biophysical profile of the full expressed recombinant mAb demonstrate a single protein of approximately 142kda under non reducing conditions and tetramer of duplexes of 23ka and 48kda, respectively. The average area under sec-HPLC curve at a wavelength of 280nm is 2490.55
Conclusions: We present the coding DNA of the recombinant panfilovirus mAb 7C8F1 and demonstrate cloning and expression of its purified extracts within CHO mammalian cell lines.
deepBreaks: a Machine Learning Tool for Identifying and Prioritizing Genotype-phenotype Associations
Authors: Baghbanzadeh Mahdi , Dawson Tyson , Sayoldin Bahar , Oakley Todd , Crandall Keith , Rahnavard Ali 
Published: February 2023

Abstract
Sequence data (e.g., nucleotides or amino-acids) are critical for advancing our understanding of biology. However, there are many challenges with investigating and analyzing sequencing data and genotype-phenotype associations, including non-independent observations, large noise components, nonlinearity, colinearity, and high dimensionality. Therefore, machine learning (ML) algorithms are well suited for analyzing sequence data as they can capture nonstructural patterns of relationships with the biology of interest as genotype-phenotype associations. Nevertheless, flexible and user-friendly implementations of ML approaches are lacking, especially ones that take advantage of the unique features of high-volume DNA sequence data. Here, we present deepBreaks, a generic approach with unified data analysis steps that identify the most important positions in sequence data that correlate with phenotypic traits of interest. deepBreaks compares the performance of multiple ML algorithms and prioritizes the most important positions based on the best-fit models. deepBreaks is open-source software with documentation available online at https://github.com/omicsEye/deepBreaks.
Factors Associated With Prolonged Pauses Between Chest Compressions in RCP With POCUS
Authors: Filho Carlos Alberto de Melo 
Published: February 2023

Abstract
Introduction: Point-of-care ultrasound (POCUS) is recommended for use in cardiopulmonary resuscitation (CPR) when reversible causes are suspected. However, studies have shown that POCUS can increase the time of pauses between compressions, in which pulse and rhythm are verified.
Objective: To describe possible factors associated with longer pauses between chest compressions with the use of POCUS.
Methodology: Methodology: Literature review in PubMed, Embase, Scopus and Biblioteca Virtual em Saúde (BVSaúde) databases. The search was performed without time or language restriction, using the following strategy: ((arrest, cardiopulmonary OR cardiopulmonary resuscitation OR Advanced Cardiac Life Support) AND (point of care ultrasound OR POCUS)).
Results and discussion: The database search resulted in 6177 publications. Of these, only five studies were included. Lack of ultrasound training is associated with longer pauses by approximately 3.1 seconds (95% CI 0.6-5.6; p-value= 0.02). Operating the POCUS and leading CPR at the same time was associated with a delay of up to 6.1 seconds (95% CI 0.4-11.7; p-value= 0.04). In addition, the time to place the ultrasound probe on the patient's chest and the lack of a systematization for POCUS use may also contribute to longer pauses.
Final thoughts: It is hoped that this discussion will spark interest in further research on the topic, as well as be used to devise new protocols for the use of POCUS in CPR to circumvent the limitations described.
Equal-time approach to real-time dynamics of quantum fields
Authors: Ott Robert , Zache Torsten V. , Berges Jürgen 
Published: February 2023

We employ the equal-time formulation of quantum field theory to
derive effective kinetic theories, first for a weakly coupled
non-relativistic Bose gas, and then for a strongly correlated system of
self-interacting NN-component
fields. Our results provide the link between state-of-the-art
measurements of equal-time effective actions using quantum simulator
platforms, as employed in [Phys. Rev. X 10, 011020 (2020); Nat. Phys.
16, 1012 (2020)], and observables underlying effective kinetic or
hydrodynamic descriptions. New non-perturbative approximation schemes
can be developed and certified this way, where the a priori time-local
formulation of the equal-time effective action has crucial advantages
over the conventional closed-time-path approach which is non-local in
time.
Removal of endocrine-disrupting chemical mixtures in water using chlorination and photolysis
Authors: Azevedo Taiza dos Santos , Santos Juliana Palermo Evangelista dos , Monteiro Neto João , Gomes Fernanda Bento Rosa , Bottrel Sue Ellen Costa , Mounteer Ann Honor , Pereira Renata de Oliveira 
Published: February 2023

Abstract
Micropollutants have been continuously detected in freshwater. In parallel, the potential adverse effects of human exposure to endocrine-disrupting chemicals (EDCs) through drinking water have been gaining the attention of researchers and health authorities. Given this fact, this study aimed to evaluate the effectiveness of chlorination and photolysis to remove the estrogenic activity caused by mixtures of EDCs in water: estrone (E1: 100 ng L−1), 17β-estradiol (E2: 100 ng L−1), ethinylestradiol (EE2: 50 ng L−1), and nonylphenol (NP: 1,000 ng L−1) under operating conditions applicable for water treatment plants. The tests were performed using freshwater spiked with the following mixtures: E1 + E2, E1 + E2 + EE2, E1 + E2 + NP, and E1 + E2 + EE2 + NP). Removal efficiencies of up to 99.7% were achieved at a chlorine dose of 2.75 mg L–1 and 30 min of contact time. In photolysis, estrogenic activity removal was higher than 99.9% at a UV dose of 186 mJ cm–2. Results indicated that both chlorination and photolysis can be efficient to remove the estrogenic activity caused by the tested EDC mixtures in water. Furthermore, experiments suggested that EDC mixtures can be efficiently removed at feasible water disinfection operating conditions.
Психологические детерминанты адаптированности в вузе студентов-первокурсников медицинского факультета в период пандемии COVID-19
Authors: Гнедых Дарья С. , Тихомирова Марина А. , Яковлева Мария В. 
Published: February 2023

Введение. Успешная адаптация студентов в университете обуславливает вовлеченность в учебную деятельность, ее эффективность, положительное отношение к выбранной профессии. Новизной исследования является изучение адаптированности студентов в ситуации перехода на новые, зачастую непривычные, формы обучения (онлайн, дистанционная) на фоне повышения общего уровня тревожности в период пандемии COVID-19. Такие трудности особенно затронули студентов медицинских специальностей, освоение которых подразумевает формирование компетенций в практической деятельности. Методы. В исследовании приняли участие студенты медицинского (N = 93) и экономического (контрольная группа, N = 111) факультетов. Были использованы следующие методики: «Адаптированность студентов в вузе» Т. Д. Дубовицкой, А. В. Крыловой, «Удовлетворенность жизнью» Э. Динера, «Тест нервно-психической адаптации» И. Н. Гурвича, «Учебные стратегии» С. Н. Костроминой, Т. А. Дворниковой, «Методика для диагностики учебной мотивации студентов» Н. Ц. Бадмаевой, «Большая пятерка» Д. П. Яничева, «Интегративный тест тревожности» А. П. Бизюка с соавт. (2 часть). Результаты и их обсуждение. К психологическим особенностям студентов первого курса медицинского факультета, способствующим адаптированности в вузе в период пандемии COVID-19, относятся: высокий уровень экстраверсии, готовность к сотрудничеству, наличие профессиональных мотивов учебной деятельности и мотивации достижения успеха, высокий уровень нервно-психической адаптации, ориентация на запоминание учебного материала и несклонность к планированию учебной деятельности. У студентов экономического факультета – это удовлетворенность жизнью, высокий уровень экстраверсии, низкий уровень тревожности, мотивы творческой самореализации и стремление к планированию в обучении. Студенты медицинского факультета с высоким уровнем адаптированности, по сравнению с высоко адаптированными студентами экономического факультета, имеют более выраженные профессиональные мотивы и чаще прибегают к стратегии повторения учебного материала. Такие различия могут быть обусловлены как особенностями организации образовательного процесса, так и спецификой выбранной специальности.
Arginine-Glycine-Aspartic Acid-anchored Curcumin-based Nanotherapeutics
Inhibit Pyroptosis-induced Cytokine Release Syndrome for In Vivo and In Vitro
Sepsis Applications
Authors: Xu Xiao-Ling , Chen Wei , Shi Yi , Wu Qian , Lu Yi , Meng Ling-Peng , Wang Xiao-Juan 
Published: February 2023

Aim:
We aimed to design RGD-anchored liposomes encapsulating an antipyroptosis drug that could efficiently target macrophages and relieve the rate of cytokine release syndrome, providing a new strategy for sepsis treatment, especially sepsis-induced acute renal injury.

Background:
Sepsis is a clinical syndrome of life-threatening organ dysfunction caused by host response disorders due to infection. Sepsis has a high incidence and remains one of the leading causes of death worldwide.

Objective:
Macrophage-mediated pyroptosis plays an important role in the occurrence and development of cytokine release syndrome and organ injury caused by sepsis. Curcumin can inhibit inflammasome assembly and slow the progression of pyroptosis by scavenging intracellular reactive oxygen species, but it has poor water solubility and low bioavailability. The emergence of drug-delivery nanosystems has overcome this problem, but there is still a lack of research on how to accurately deliver antipyroptotic drugs to innate immune cells and subsequently hinder pyroptosis.

Method:
We constructed a curcumin-loaded RGD-modified liposome (RGD-lipo/Cur) and demonstrated that RGD-lipo/Cur could effectively target macrophages.

Result:
In vitro, RGD-lipo/Cur reduced the upregulation of caspase-1, caspase-3, NLRP3, IL-1β and GSDMD, inhibiting pyroptosis, reducing oxidative stress, and attenuating the proinflammatory cytokine cascade.

Conclusion:
RGD-lipo/Cur was considered to have great potential for sepsis treatment.