bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and functional validation of SRC and RAPGEF1 as new direct targets of miR-203, involved in regulation of epidermal homeostasis
Authors: Golebiewski Christelle , Gastaldi Cécile , Vieu Diane-Lore , Mari Bernard , Rezzonico Roger , Bernerd Françoise , Marionnet Claire 
Published: August 2023

AbstractThe epidermis is mostly composed of keratinocytes and forms a protecting barrier against external aggressions and dehydration. Epidermal homeostasis is maintained by a fine-tuned balance between keratinocyte proliferation and differentiation. In the regulation of this process, the keratinocyte-specific miR-203 microRNA is of the outmost importance as it promotes differentiation, notably by directly targeting and down-regulating mRNA expression of genes involved in keratinocyte proliferation, such as ΔNp63, Skp2 and Msi2. We aimed at identifying new miR-203 targets involved in the regulation of keratinocyte proliferation/differentiation balance. To this end, a transcriptome analysis of human primary keratinocytes overexpressing miR-203 was performed and revealed that miR-203 overexpression inhibited functions like proliferation, mitosis and cell cycling, and activated differentiation, apoptosis and cell death. Among the down-regulated genes, 24 putative target mRNAs were identified and 8 of them were related to proliferation. We demonstrated that SRC and RAPGEF1 were direct targets of miR-203. Moreover, both were down-regulated during epidermal morphogenesis in a 3D reconstructed skin model, while miR-203 was up-regulated. Finally silencing experiments showed that SRC or RAPGEF1 contributed to keratinocyte proliferation and regulated their differentiation. Preliminary results suggest their involvement in skin carcinoma hyperproliferation. Altogether this data indicates that RAPGEF1 and SRC could be new mediators of miR-203 in epidermal homeostasis regulation.
Role of mucin glycosylation in the gut microbiota-brain axis of core 3 O-glycan deficient mice
Authors: Coletto Erika , Savva George M. , Latousakis Dimitrios , Pontifex Matthew , Crost Emmanuelle H. , Vaux Laura , Telatin Andrea , Bergstrom Kirk , Vauzour David , Juge Nathalie 
Published: August 2023

AbstractAlterations in intestinal mucin glycosylation have been associated with increased intestinal permeability and sensitivity to inflammation and infection. Here, we used mice lacking core 3-derived O-glycans (C3GnT−/−) to investigate the effect of impaired mucin glycosylation in the gut-brain axis. C3GnT−/− mice showed altered microbial metabolites in the caecum associated with brain function such as dimethylglycine and N-acetyl-l-tyrosine profiles as compared to C3GnT+/+ littermates. In the brain, polysialylated-neural cell adhesion molecule (PSA-NCAM)-positive granule cells showed an aberrant phenotype in the dentate gyrus of C3GnT−/− mice. This was accompanied by a trend towards decreased expression levels of PSA as well as ZO-1 and occludin as compared to C3GnT+/+. Behavioural studies showed a decrease in the recognition memory of C3GnT−/− mice as compared to C3GnT+/+ mice. Combined, these results support the role of mucin O-glycosylation in the gut in potentially influencing brain function which may be facilitated by the passage of microbial metabolites through an impaired gut barrier.
Deforestation within breeding ranges may still drive population trends of migratory forest birds in the East Asian Flyway
Authors: Ko Jerome Chie-Jen , Chang An-Yu , Lin Ruey-Shing , Lee Pei-Fen 
Published: August 2023

AbstractThe East Asian Flyway (EAF) is the most species diverse of global flyways, with deforestation in its migratory landbird’s non-breeding range suspected to be the main driver of population decline. Yet range-wide habitat loss impact assessments on EAF migratory landbirds are scarce, and seasonal variation in habitat preference of migratory species further increases the complexity for conservation strategies. In this study, we reviewed population trends of migratory forest breeding birds in the EAF along with their seasonal habitat preference from the literature and assessed the impact of forest cover change in species’ breeding and non-breeding ranges on population trends. We found that 41.3% of the bird species with trend data available are declining, and most have higher forest preference in the breeding season. Despite 93.4% of the species experienced deforestation throughout their annual cycle, forest cover change in the non-breeding range was not identified as the main driver of population trend. However, forest cover change in species’ regional breeding range interacts positively with the degree of breeding season forest preference in predicting population trends. We therefore stress that regional breeding habitat protection may still be important while following the call for cross-border collaboration to fill the information gap for flyway conservation.
Prevalence and molecular characterization of Giardia duodenalis in dairy cattle in Central Inner Mongolia, Northern China
Authors: Zhao Li , Zhang Zhan-Sheng , Han Wen-Xiong , Yang Bo , Chai Hai-Liang , Wang Ming-Yuan , Wang Yan , Zhang Shan , Zhao Wei-Hong , Ma Yi-Min , Zhan Yong-Jie , Wang Li-Feng , Ding Yu-Lin , Wang Jin-Ling , Liu Yong-Hong 
Published: August 2023

AbstractGiardia duodenalisis a gastrointestinal protozoan ubiquitous in nature. It is a confirmed zoonotic pathogen, and cattle are considered a source of giardiasis outbreaks in humans. This study aimed to evaluate the prevalence and multilocus genotype (MLG) ofG. duodenalisin dairy cattle in Central Inner Mongolia. This study was based on the small subunit ribosomal RNA (SSU rRNA), glutamate dehydrogenase (gdh), triosephosphate isomerase (tpi), and beta-giardin (bg) genes ofG. duodenalis. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed on 505 dairy cattle fecal samples collected in 2021 from six sampling sites and four age groups in Central Inner Mongolia to determine the prevalence and MLG distribution ofG. duodenalis. The PCR results ofSSU rRNArevealed that the overall prevalence ofG. duodenaliswas 29.5% (149/505) and that the overall prevalence of the diarrhea and nondiarrhea samples was 31.5% (46/146) and 28.5% (103/359), respectively; the difference was not significant (p > 0.05).SSU rRNAsequence analysis revealed thatG. duodenalisassemblage E (91.1%, 133/146) was primarily detected and that assemblage A (8.9%, 13/146) was detected in 13 samples. TheG. duodenalis—positive samples were PCR amplified and sequenced forgdh,tpi, andbg, from which 38, 47, and 70 amplified sequences were obtained, respectively. A combination ofG. duodenalisassemblages A and E were detected in seven samples. Multilocus genotyping yielded 25 different assemblage E MLGs, which formed six subgroups. To the best of our knowledge, this is the first report regardingG. duodenalisinfection in dairy cattle in Inner Mongolia, China. This study revealed that Inner Mongolian cattle pose a risk of giardiasis transmission to humans and that the distribution of local cattleG. duodenalisassemblage E MLGs is diverse. The findings of this study can bridge the knowledge gap in the molecular epidemiological investigation of giardiasis in Central Inner Mongolia.
Drug–drug interaction signals between loop diuretics and teicoplanin during acute kidney injury evaluated using Japanese spontaneous adverse drug event reports
Authors: Hirai Toshinori , Kondo Yuki , Sakazaki Yuka , Seki Ayaka , Ishitsuka Yoichi , Iwamoto Takuya 
Published: August 2023

AbstractTeicoplanin can cause acute kidney injury, but little is known about the risk of acute kidney injury when teicoplanin is co-administered with loop diuretics (a powerful diuresis), which can alter renal hemodynamics and glomerular filtration rate. We performed a signal detection analysis using a Japanese adverse event database to determine the additive impact of loop diuretics on acute kidney injury associated with teicoplanin. The dataset originated between April 2004 and August 2022. Disproportionality analysis was performed to detect the signals for acute kidney injury (the Standardized MedDRA Query) when co-administered teicoplanin or vancomycin (a positive control) with individual diuretics, including loop diuretics. Multivariate logistic regression analysis was tested to estimate the adjusted reporting odds ratio (aROR) and 95% confidence interval (95% CI). There were 147 and 515 events of acute kidney injury associated with teicoplanin and vancomycin, respectively. A significant positive signal for acute kidney injury when teicoplanin was co-administered with loop diuretics was present (aROR 4.83, 95% CI 3.52–6.61, p < 0.0001). Contrastingly, no significant signals were observed when vancomycin was co-administered with any diuretics. These findings suggest that co-administered loop diuretics may have an unfavorable effect on acute kidney injury while undertaking teicoplanin but not vancomycin.
Tm3+/Ho3+ profiled co-doped core area optical fiber for emission in the range of 1.6–2.1 µm
Authors: Miluski Piotr , Markowski Krzysztof , Kochanowicz Marcin , Łodziński Marek , Żmojda Jacek , Pisarski Wojciech A. , Pisarska Joanna , Kuwik Marta , Leśniak Magdalena , Dorosz Dominik , Ragiń Tomasz , Askirka Valiantsin , Dorosz Jan 
Published: August 2023

AbstractDouble-clad optical fiber with a multi-ring core profile doped with thulium and holmium fabricated by Modified Chemical Vapor Deposition Chelate Doping Technology (MCVD-CDT) is presented. The measured Tm2O3 and Ho2O3 complexes’ weight concentrations were 0.5% and 0.2% respectively. Numerical analyses show weakly guiding conditions and 42.2 µm of MFD LP01 at 2000 nm. The low NA numerical aperture (NA = 0.054) was obtained for the 20/250 µm core/cladding ratio optical fiber construction. The emission spectra in the range of 1.6–2.1 µm vs. the fiber length are presented. The full width at half maximum (FWHM) decreases from 318 to 270 nm for fiber lengths from 2 to 10 m. The presented fiber design is of interest for the development of new construction of optical fibers operating in the eye-safe spectral range.
Microsurgical robotic system enables the performance of microvascular anastomoses: a randomized in vivo preclinical trial
Authors: Malzone Gerardo , Menichini Giulio , Innocenti Marco , Ballestín Alberto 
Published: August 2023

AbstractTechnical advances in microsurgery have enabled complex oncological reconstructions by performing free tissue transfers, nerve and lymphatic reconstructions. However, the manual abilities required to perform microsurgery can be affected by human fatigue and physiological tremor resulting in tissue damage and compromised outcomes. Robotic assistance has the potential to overcome issues of manual microsurgery by improving clinical value and anastomoses’ outcomes. The Symani Surgical System, a robotic platform designed for microsurgery, was used in this in-vivo preclinical study using a rat animal model. The tests included anastomoses on veins and arteries performed by microsurgeons manually and robotically, with the latter approach using Symani. The anastomoses were assessed for patency, histopathology, and execution time. Patency results confirmed that the robotic and manual techniques for venous and arterial anastomoses were equivalent after anastomosis, however, the time to perform the anastomosis was longer with the use of the robot (p < 0.0001). Histological analysis showed less total average host reaction score at the anastomotic site in robotic anastomosis for both veins and arteries. This study demonstrates the equivalence of vessel patency after microsurgical anastomoses with the robotic system and with manual technique. Furthermore, robotic anastomosis has proven to be slightly superior to manual anastomosis in terms of decreased tissue damage, as shown by histological analysis.
A single-mode tunable plasmonic sensor based on an 8-shaped resonator for cancer cell detection
Authors: Danaie Mohammad , Hajshahvaladi Leila , Ghaderpanah Elham 
Published: August 2023

AbstractIn this paper, a novel 8-shaped resonator coupled to metal–insulator–metal waveguides is used for designing plasmonic filters and sensors. The resonator supports two resonance modes, which result in peaks in the transmission spectrum of the structure. A Q-factor of 247.4 which can reach up to 270 at the wavelength of 1187.5 nm is observed. By placing vertical and horizontal metal blades in the resonator, two tunable single-mode plasmonic filters are obtained at the first and second resonance modes, respectively. The effect of structural parameters on the transmission spectrum is investigated using the finite-difference time-domain (FDTD) method. Based on the obtained results, the proposed plasmonic structure can be used for biosensing applications such as the detection of basal cancer cells with a sensitivity of 1200 nm/RIU. It is of great significance that both the sensitivity and Q-factor values for the proposed structure are higher than most recent sensors reported in the literature. Therefore, the proposed structure is a potentially promising candidate for filtering and sensing applications.
Fatty acid composition and genome-wide associations of a chickpea (Cicer arietinum L.) diversity panel for biofortification efforts
Authors: Salaria Sonia , Boatwright J. Lucas , Johnson Nathan , Madurapperumage Amod , Joshi Priyanka , Thavarajah Pushparajah , Vandemark George , Thavarajah Dil 
Published: August 2023

AbstractChickpea is a nutritionally dense pulse crop with high levels of protein, carbohydrates, micronutrients and low levels of fats. Chickpea fatty acids are associated with a reduced risk of obesity, blood cholesterol, and cardiovascular diseases in humans. We measured four primary chickpea fatty acids; palmitic acid (PA), linoleic acid (LA), alpha-linolenic acid (ALA), and oleic acid (OA), which are crucial for human health and plant stress responses in a chickpea diversity panel with 256 accessions (Kabuli and desi types). A wide concentration range was found for PA (450.7–912.6 mg/100 g), LA (1605.7–3459.9 mg/100 g), ALA (416.4–864.5 mg/100 g), and OA (1035.5–1907.2 mg/100 g). The percent recommended daily allowances also varied for PA (3.3–6.8%), LA (21.4–46.1%), ALA (34.7–72%), and OA (4.3–7.9%). Weak correlations were found among fatty acids. Genome-wide association studies (GWAS) were conducted using genotyping-by-sequencing data. Five significant single nucleotide polymorphisms (SNPs) were identified for PA. Admixture population structure analysis revealed seven subpopulations based on ancestral diversity in this panel. This is the first reported study to characterize fatty acid profiles across a chickpea diversity panel and perform GWAS to detect associations between genetic markers and concentrations of selected fatty acids. These findings demonstrate biofortification of chickpea fatty acids is possible using conventional and genomic breeding techniques, to develop superior cultivars with better fatty acid profiles for improved human health and plant stress responses.
Circularly polarized printed dual port MIMO antenna with polarization diversity optimized by machine learning approach for 5G NR n77/n78 frequency band applications
Authors: Dwivedi Ajay Kumar , Narayanaswamy Nagesh Kallollu , Penmatsa Krishna Kanth Varma , Singh Suyash Kumar , Sharma Anand , Singh Vivek 
Published: August 2023

AbstractIn this communication, a planar dual port multiple input multiple output antenna of size 1.2λ0 × 0.6λ0 × 0.008λ0 with LHCP/RHCP features is reported for the fifth-generation new radio n77/n78 sub-6 GHz wireless applications band. The single unit of the proposed design consists of a modified L-shape rectangular radiator with Z-shape slot loaded DGS. The defected ground structure is optimized through machine learning algorithms to achieve the maximum ARBW (output) by Right Shifting (RS) and left shifting (LS) the DGS and obtaining input features. The performance metric for ANN with ADAM optimizer was found to be optimal with MSE and R2 of 0.99 and 0.82, respectively. ANNs can leverage gradient information to guide the optimization process. This enables faster convergence towards optimal solutions compared to popular GAs and PSO, which are often gradient-free optimization methods. The MIMO configuration is achieved by creating a mirror image of the single unit about the x-axis. The salient features of the proposed design are (a) Impedance bandwidth (IBW) of 3.0–4.2 GHz covering the n77/n78 band, (b) 3-dB axial ratio bandwidth (ARBW) of the 2.6–3.9 GHz (c) Port-1 is generating RHCP while Port-2 is generating LHCP, results in polarization diversity. Different diversity performance parameters (ECC < 0.005, DG ~ 9.99 dB, and MEG < 3 dB) are in the optimum range confirming the proposed configuration as a suitable design for a MIMO radiator.
A process-based assessment of the sea-level rise in the northwestern Pacific marginal seas
Authors: Cha Hyeonsoo , Moon Jae-Hong , Kim Taekyun , Song Y. Tony 
Published: August 2023

AbstractBecause regional sea-level rise can threaten coastal communities, understanding and quantifying the underlying process contributing to reginal sea-level budget are essential. Here, we assessed whether the regional sea-level rise on the northwestern Pacific marginal seas can be closed with a combination of observations and ocean reanalyses over 1993–2017, as well as with independent observations from in situ profiles including Argo floats and satellite gravity measurements since 2003. The assessment represents that the major contributions come from the land ice melt and sterodynamic components, while the spatial pattern and interannual variability are dominated by sterodynamic effect. The observation-based estimate further shows that along continental shelves, sterodynamic sea-level changes are substantially induced by ocean mass redistribution due to changes in ocean circulation. This result highlights the ocean mass change between the deep ocean and shallow marginal seas, which plays a role in driving regional sea-level rise and variability.
11 b 200 MS/s 28‐nm CMOS 2b/cycle successive‐approximation register analogue‐to‐digital converter using offset‐mismatch calibrated comparators
Authors: Lee Jaehyuk , Boo Junho , Park Junsang , An Taiji , Shin Heewook , Cho Youngjae , Choi Michael , Burm Jinwook , Ahn Gilcho , Lee Seunghoon 
Published: August 2023

AbstractThis letter presents an 11 b 200 MS/s 28 nm CMOS 2b/cycle successive‐approximation register (SAR) analogue‐to‐digital converter (ADC). The offset calibration technique is proposed to reduce the comparator offset mismatch that degrades the linearity of the high‐resolution 2b/cycle SAR ADC. The offset mismatch is reduced to within 0.25 least significant bit (LSB) by generating a compensation voltage from capacitor‐resistor (C‐R) hybrid digital‐to‐analogue converters (DACs). The prototype ADC implemented in a 28‐nm CMOS process demonstrates measured differential and integral non‐linearities within 0.6 LSB and 1.73 LSB at 11 b resolution, respectively. The measured signal‐to‐noise‐and‐distortion ratio (SNDR) and spurious‐free dynamic range (SFDR) are 50.9 dB and 66.2 dB at Nyquist, respectively. The prototype ADC occupies an active die area of 0.115 mm2 and consumes 3.98 mW at a 1.1‐V supply voltage.
Unusually long path length for a nearly scatter-free solar particle event observed by Solar Orbiter at 0.43 au
Authors: Wimmer-Schweingruber Robert F. , Berger Lars , Kollhoff Alexander , Kühl Patrick , Heber Bernd , Yang Liu , Heidrich-Meisner Verena , Klassen Andreas , Gomez-Herrero Raúl , Rodriguez-Pacheco Javier , Ho George C. , Mason Glenn M. , Janitzek Nils P. , Kouloumvakos Athanasios , Wang Linghua , Warmuth Alexander , Lario David , Carcaboso Fernando , Owen Christopher J. , Bučík Radoslav , Pacheco Daniel , Malandraki Olga , Allen Robert C. , Rodriguez Luciano , Shukhobodskaia Daria , Espinosa Lara Francisco , Cernuda Ignacio , Böttcher Stephan I. , Eldrum Sandra , Fleth Sebastian , Xu Zigong 
Published: August 2023

Context. After their acceleration and release at the Sun, solar energetic particles (SEPs) are injected into the interplanetary medium and are bound to the interplanetary magnetic field (IMF) by the Lorentz force. The expansion of the IMF close to the Sun focuses the particle pitch-angle distribution, and scattering counteracts this focusing. Solar Orbiter observed an unusual solar particle event on 9 April 2022 when it was at 0.43 astronomical units (au) from the Sun.
Aims. We show that the inferred IMF along which the SEPs traveled was about three times longer than the nominal length of the Parker spiral and provide an explanation for this apparently long path.
Methods. We used velocity dispersion analysis (VDA) information to infer the spiral length along which the electrons and ions traveled and infer their solar release times and arrival direction.
Results. The path length inferred from VDA is approximately three times longer than the nominal Parker spiral. Nevertheless, the pitch-angle distribution of the particles of this event is highly anisotropic, and the electrons and ions appear to be streaming along the same IMF structures. The angular width of the streaming population is estimated to be approximately 30 degrees. The highly anisotropic ion beam was observed for more than 12 h. This may be due to the low level of fluctuations in the IMF, which in turn is very probably due to this event being inside an interplanetary coronal mass ejection The slow and small rotation in the IMF suggests a flux-rope structure. Small flux dropouts are associated with very small changes in pitch angle, which may be explained by different flux tubes connecting to different locations in the flare region.
Conclusions. The unusually long path length along which the electrons and ions have propagated virtually scatter-free together with the short-term flux dropouts offer excellent opportunities to study the transport of SEPs within interplanetary structures. The 9 April 2022 solar particle event offers an especially rich number of unique observations that can be used to limit SEP transport models.
Oort Cloud comets discovered far from the Sun
Authors: Królikowska Małgorzata , Dones Luke 
Published: August 2023

Context. Increasingly, Oort Cloud comets are being discovered at great distances from the Sun and tracked over ever wider ranges of heliocentric distances as observational equipment improves.
Aims. Our goal is to investigate in detail how the original semimajor axis for near-parabolic comets depends on the selected data arc and the assumed form of the nongravitational (NG) acceleration.
Methods. Among currently known Oort Cloud comets with large perihelion distances (q > 3 au), we selected 32 objects observed over the widest ranges of heliocentric distances in orbital legs before and after perihelion. For each of them, we determined a series of orbits using at least three basic types of datasets selected from available positional data (pre- and post-perihelion data and the entire dataset), and a few forms of NG acceleration representing water ice or CO sublimation.
Results. We find that the motion of comets is often measurably affected by NG forces at heliocentric distances beyond 5 au from the Sun. The most spectacular example is C/2010 U3 (Boattini), whose perihelion distance is 8.44 au. NG effects are detectable for 19 of the 32 comets within the positional data. For five comets, we find asymmetric effects of NG forces – in three cases significantly greater before perihelion than afterward (C/2017 M4, C/2000 SV75, and C/2015 O1), and in two others the opposite (C/1997 BA6 and C/2006 S3). We also find that the well-known systematic effect of finding more tightly bound original orbits when including the NG acceleration than in purely gravitational solutions may be related to the specific form of the standard g(r) function describing the sublimation of ices.