bluuebunny/crossref_metadata_2025_split huggingface dataset:
High numerical aperture imaging allows chirality measurement in individual collagen fibrils using polarization second harmonic generation microscopy
Authors: Harvey MacAulay , Cisek Richard , Alizadeh Mehdi , Barzda Virginijus , Kreplak Laurent , Tokarz Danielle 
Published: April 2023

Abstract
Second harmonic generation (SHG) microscopy is a commonly used technique to study the organization of collagen within tissues. However, individual collagen fibrils, which have diameters much smaller than the resolution of most optical systems, have not been extensively investigated. Here we probe the structure of individual collagen fibrils using polarization-resolved SHG (PSHG) microscopy and atomic force microscopy. We find that longitudinally polarized light occurring at the edge of a focal volume of a high numerical aperture microscope objective illuminated with linearly polarized light creates a measurable variation in PSHG signal along the axis orthogonal to an individual collagen fibril. By comparing numerical simulations to experimental data, we are able to estimate parameters related to the structure and chirality of the collagen fibril without tilting the sample out of the image plane, or cutting tissue at different angles, enabling chirality measurements on individual nanostructures to be performed in standard PSHG microscopes. The results presented here are expected to lead to a better understanding of PSHG results from both collagen fibrils and collagenous tissues. Further, the technique presented can be applied to other chiral nanoscale structures such as microtubules, nanowires, and nanoribbons.
Chloride ion transport and service life prediction of aeolian sand concrete under dry–wet cycles
Authors: Dong Wei , Ji Yajing 
Published: April 2023

Abstract
In this study, the dry–wet cycle test of chloride salt was carried out on aeolian sand concrete with different contents, and the chloride ion content in aeolian sand concrete was determined by taking powder from different depths on one side of the test block. And then combined with Monte Carlo stochastic statistical simulation and Weibull probability distribution function, the service life prediction model of aeolian sand concrete against chloride ion erosion is established. The results show that the free chloride ion content in aeolian sand concrete decreases with the increase in the depth from the surface of the specimen. At the same depth from the surface of the specimen, the free chloride ion content gradually increases with the increase in the number of dry–wet cycles. Through the analysis of life prediction, it is concluded that with the increase in aeolian sand content, the service life of aeolian sand concrete increases first and then decreases. The service life value of concrete with 75% aeolian sand content is the largest, and the greater the thickness of the protective layer, the more favorable the service life value.
LncRNA FLAIL affects alternative splicing and represses flowering in Arabidopsis
Authors: Jin Yu , Ivanov Maxim , Dittrich Anna Nelson , Nelson Andrew DL , Marquardt Sebastian 
Published: April 2023

AbstractHow the noncoding genome affects cellular functions is a key biological question. A particular challenge is to distinguish the effects of noncoding DNA elements from long noncoding RNAs (lncRNAs) that coincide at the same loci. Here, we identified the flowering‐associated intergenic lncRNA (FLAIL) in Arabidopsis through early flowering flail mutants. Expression of FLAIL RNA from a different chromosomal location in combination with strand‐specific RNA knockdown characterized FLAIL as a trans‐acting RNA molecule. FLAIL directly binds to differentially expressed target genes that control flowering via RNA–DNA interactions through conserved sequence motifs. FLAIL interacts with protein and RNA components of the spliceosome to affect target mRNA expression through co‐transcriptional alternative splicing (AS) and linked chromatin regulation. In the absence of FLAIL, splicing defects at the direct FLAIL target flowering gene LACCASE 8 (LAC8) correlated with reduced mRNA expression. Double mutant analyses support a model where FLAIL‐mediated splicing of LAC8 promotes its mRNA expression and represses flowering. Our study suggests lncRNAs as accessory components of the spliceosome that regulate AS and gene expression to impact organismal development.
Interventions in the Home and Community for Medically Complex Children: A Systematic Review
Authors: Watkinson Michelle D. , Ehlenbach Mary , Chung Paul J. , Kelly Michelle , Werner Nicole , Jolliff Anna , Katz Barbara , Marleau Heidi , Nacht Carrie L. , Warner Gemma , Coller Ryan J. 
Published: April 2023

CONTEXT
Most care occurs in home and community settings; however, the best approaches to improve CMC health are poorly understood.

OBJECTIVE
We sought to summarize evidence from interventions in the home and community to improve health for children with medical complexity (CMC) using comprehensive conceptions of CMC health.

DATA SOURCES
PubMed, CINAHL, Scopus, and Cochrane databases.

STUDY SELECTION
Included studies evaluated interventions for CMC caregivers in home or community settings and evaluated at least 1 outcome in 10 domains of CMC health.

DATA EXTRACTION
Data were extracted on participant characteristics, intervention activities, and outcomes. Interventions were categorized thematically into strategies, with results summarized by effects on outcomes within each health domain.

RESULTS
The 25 included interventions used 5 strategies: intensive caregiver education (n = 18), support groups (n = 3), crisis simulation (n = 2), mobile health tracking (n = 1), and general education (n = 1). Substantial variation existed in the extent to which any outcome domain was studied (range 0–22 studies per domain). Interventions addressing 4 domains showed consistent improvement: support group and mobile health tracking improved long-term child and caregiver self-sufficiency; mobile health tracking improved family-centered care; intensive caregiver education and support groups improved community system supports. Three domains (basic needs, inclusive education, patient-centered medical home) were not studied.

LIMITATIONS
Risk of bias was moderate due primarily to limited controlled experimental designs and heterogeneous population and outcome definitions.

CONCLUSIONS
Interventions that improve CMC health exist; however, current studies focus on limited segments of the 10 domains framework. Consensus outcome measures for CMC health are needed.
CT and MRI findings of primary hepatic giant cell tumor of soft tissue: A case report
Authors: He Lei , Zhang Yalin 
Published: April 2023

AbstractPrimary giant cell tumor of soft tissue (GCT-ST) is a low-grade malignant tumor and uncommon cancer originating from soft tissue. Primary hepatic GCT is an extremely rare finding. Only a handful of cases have been reported in the literature. Although GCT-ST has a relatively low malignant potential tumor in the view of histology, some patients who suffered from this tumor have poor prognosis after surgery because of the aggressiveness. Thus, the early diagnosis is important, and a preoperative imaging can supply the evidence of differential diagnosis to evaluate tumor malignancy. In this study, we systematically present a rare case of 56-year-old women with chills, fever, jaundice and anorexia for 3 days. CT showed a huge and multinodular confluent lesion in the left lobe of the liver, and it had a calcification component. This tumor is characterized by fibrous components and calcification, which showed iso-intense with peripheral hypo-intense on T1WI, and mainly inhomogeneous hypo-intense on fat-suppressed T2WI. Moreover, it was mainly hypointense and peripheral linear hyperintense on DWI images with a b-value of 800 (b800) and iso-hyperintense on ADC.
Spatial–Temporal Distribution and Focal Mechanisms for the Shallow Earthquakes in the Rongchang-Luxian Region in Southern Sichuan Basin, China
Authors: Lai Guijuan , Jiang Changsheng , Wang Weilai , Lei Xinglin , Zhang Long , Cai Guangyao , Gong Haobo , Guo Xiangyun , Chen Mingfei 
Published: April 2023

AbstractAn Ms 6.0 earthquake struck the Luxian County in southern Sichuan basin, China, on 15 September 2021, which was the largest local event thus far and caused heavy casualties and serious property losses. Using data of 107 temporary and six permanent stations from five months before to four and a half months after the time of the mainshock in the Rongchang-Luxian region, we utilize the machine learning-based workflow for phase picking, earthquake detection and relocation, and obtain precise hypocenter data for 22,806 events. We found that earthquakes increase significantly since July 2021, showing north-northeast-, northeast-, east-northeast- and east-southeast-directed seismic strips. Almost all events are located within the sedimentary layers at 1–7 km depth above the crystalline basement, and more than half of them occurred in Silurian and Ordovician formations. The Ms 6.0 Luxian earthquake sequence occurred in an area where no clear seismicity was observed before the mainshock. On map view, the aftershock zone shows a convex polygon with an overall strike of 112°. The focal depths of aftershocks southwest to the mainshock are distributed in a narrower range of 4–7 km and extend to a wider range of 2–7 km on the northeast side. It is speculated that the seismogenic fault is a blind thrust fault dipping to the southwest at 2–7 km depth. The focal mechanisms of 13 ML 3.0+ earthquakes show either pure reverse faulting or reverse faulting dominated mechanisms with a strike-slip component. The centroid depths fall in the range of 1.95–6.75 km. The maximum and middle principal stress axes are nearly horizontal; the azimuth of the maximum horizontal stress is about 120° and the stress shape ratio is 0.78. These results provide an important reference for reunderstanding the local seismic risk and for detailed studies on the seismological aspects about the unusual Luxian earthquake in the future.
Solução analítica aproximada para convecção forçada de metais líquido
Authors: Santos Jacques Cesar Dos , Silva Samuel Alves da 
Published: April 2023

<p>O presente trabalho apresenta um estudo teórico da convecção forçada interna em um duto circular com condução axial no fluido, realizado através da Técnica da Transformada integral. Uma solução analítica aproximada de baixo custo computacional é obtida. É realizada uma análise gráfica e numérica para o problema.</p>
Teoria argumentacji w ujęciu Chaima Perelmana
Authors: Kleszcz Ryszard 
Published: April 2023

Le travail est consacré á la caractérisation de la théorie ď'argumentation créée par Perelman. Ce philosophe belge veut traitor la philosophie d’une façon ample, trouvant qu'elle doit aussi construire les principes de ľ'activité humaine, íl fáut que ces principes soient raisonnables et mútivés. Selon Perelman la logique contemporaine ne favorise pas сеs aspirations. Souvent elle est reduite à la logique formelle (A, Church, J. Bocheński), laissant en dehorn de ses intéréts ces moyens de raisonnemont qu’on applique a'laboration de la décision. Perelman n'accepte pas ces conceptions, se reférant à Aristote. La théorie d ’argumentation proposée par lui , qu'on appelle rhétorique nouvelle, comprend ces techniques de raisonnement à l'aide desquelles on tend a faire accepter par l'auditoire donné la these présentée. Ces méthodes se trouvent à l'extérieur de la logique formelle et ne concement pas les situations dans les quelles on peut recourir a la vérification empirique. Elles sont langement appliquées dans les discussions philosophiques, morales, politiques et aussi juridiques.
Fièvre de la vallée du Rift en Tunisie : Synthèse sur la situation actuelle et perspectives
Authors: Mejri Selma 
Published: April 2023

La fièvre de la vallée du Rift (FVR) est une infection zoonotique émergente infectant les humains et les ruminants. L’agent étiologique de cette infection est le virus de la FVR, un arbovirus transmis principalement par les piqûres de moustiques des genres Aedes et Culex. Durant les deux dernières décennies, des cas de FVR ont été rapportés dans des régions jusque-là indemnes et les épidémies sont devenues de plus en plus fréquentes. De par sa localisation stratégique dans le bassin méditerranéen, la Tunisie a de nombreux échanges avec les autres pays africains ainsi qu’avec l’Europe. L’analyse de la situation épidémiologique de la FVR dans ce pays pourrait aider à comprendre la situation globale de cette infection dans la région méditerranéenne et à élaborer des stratégies efficaces de surveillance à mettre en œuvre dans la région. Dans ce cadre et afin d’étudier l’état des lieux de la FVR en Tunisie, cette synthèse présente une analyse à partir de tous les articles scientifiques traitant de ce sujet dans le pays. La Tunisie est considérée comme à haut risque de propagation de la FVR en raison de sa localisation, de ses caractéristiques climatiques et environnementales, de l’abondance des vecteurs transmettant le virus de la FVR, et de la présence d’espèces animales à risque pour cette maladie. Ainsi, des mesures strictes doivent être prises afin de contrôler et de limiter toute émergence et propagation de la FVR. De telles mesures doivent inclure le contrôle des mouvements des animaux (particulièrement aux frontières), le contrôle des espèces vectrices du virus, et le perfectionnement des outils de diagnostic pour la détection précoce de tout cas suspect. L’implémentation d’une approche « Une seule santé » multidisciplinaire serait l’une des meilleures solutions pour faire face aux maladies zoonotiques à transmission vectorielle comme la FVR.
PERAN TAMAN HUTAN HUJAN TROPIS INDONESIA TERHADAP IKLIM MIKRO (Studi Kasus di Taman Hutan Hujan Tropis Indonesia Kawasan Kantor Gubernur di Kota Banjarbaru Provinsi Kalimantan Selatan)
Authors: Raihan Fathur , Akbar Arief R. M. , Indrayatie Eko Rini , Kissinger Kissinger 
Published: April 2023

In line with the increasing population and the community's need for city facilities and infrastructure, urban development continues to increase. The paradigm of urban development should have changed and needs to be designed early towards the city with a better, more balanced way of life and still paying attention to environmental quality. The level of environmental comfort decreases as a result of the imbalance between built-up space and green open space (RTH) with the three main functions of urban vegetation being structural function, environmental function and visual function. RTH has a role in shaping the comfort of the urban climate through ecological functions, namely as a regulator of air temperature and humidity, so that it can be used as input in improving the quality of the microclimate by improving the quality of urban RTH so as to minimize the decline in environmental quality. The purpose of the study was to analyze the influence of vegetation structure and the role in improving the microclimate at each Tropical Rainforest Miniature RTH location in the Governor's Office area in Banjarbaru City. Banua Botanical Garden as a research site located in the Banjarbaru City Area, South Kalimantan Provincial Government Office Complex. The influence of vegetation structure on Location I which is dominated by lawns/grasses and shrubs with horizontal and columnar header shapes with low header density. Meanwhile, in Location II, the shape of the canopy is columnar and horizontal with a medium canopy density and has a tree height of 3-4 meters, slightly reducing the air temperature around the RTH. For Locations III and IV, they have a pyramid-shaped and spherical header shape that acts as a counterweight to increasing air humidity and decreasing air temperature. The role of the Tropical Rainforest Miniature RTH in the Governor's Office area in Banjarbaru City in improving the microclimate based on air temperature at locations I, II, III and IV, namely 30.1°C, 29.2°C, 28.0°C and 27.3°C. It can be seen that location III and location IV have the lowest temperature because they have characteristics of vegetation structures that can lower air temperature. For humidity parameters, it will adjust to air temperature conditions as well as wind speed will adjust to the vegetation structure in the RTH.
Differences in the Academic Attributes of Matched and Unmatched Orthopaedic Surgery Residency Applicants are Narrowing
Authors: Sudah Suleiman Y. , Imam Nareena , Sirch Francis , Nicholson Allen D. , Namdari Surena , Menendez Mariano E. 
Published: April 2023

Background:
Orthopaedic surgery remains one of the most competitive residency specialties, with the number of applicants outpacing the availability of residency positions each year. The purpose of this study was to analyze present-day orthopaedic surgery match data, identify differences between matched and unmatched applicants, and compare our findings to previous trends.

Methods:
Applicant data from the National Resident Matching Program from 2016 to 2022 were analyzed. The number of matched and unmatched US allopathic senior orthopaedic applicants relative to the number of available positions was used to determine respective match rates. Performance metrics and applicant characteristics were compared by match status. Trends were compared with those of previous analysis from 2006 to 2014.

Results:
The number of applicants increased from 863 in 2016 to 1,068 in 2022. The match rate decreased from 75% in 2016 to 66% in 2022 (p < 0.0001). Matched applicants had a higher number of contiguous ranks (12.3 vs. 6.5; p < 0.001), United States Medical Licensing Examination (USMLE) Step-1 score (248 vs. 240; p < 0.001), USMLE Step-2 score (255 vs. 247; p < 0.001), Alpha Omega Alpha (AOA) membership (38% vs. 13%; p < 0.001), and enrollment at a top 40 National Institutes of Health (NIH)-funded medical school (34% vs. 24%, p < 0.001). Compared with 2006 to 2014 data, a smaller percentage of matched applicants were enrolled in a top 40 NIH-funded medical school (34% vs. 37%, p = 0.013). The mean differences in USMLE Step-1 score (16 vs. 8.25 points, p < 0.001) and USMLE Step-2 score (16 vs. 8.25 points, p = 0.002) in favor of matched applicants nearly halved compared with that in 2006 to 2014. In addition, there was no longer a significant difference in the number of research products (abstracts, presentations, posters, and publications) between matched and unmatched applicants (p = 0.309).

Conclusions:
Differences in the academic attributes of matched and unmatched orthopaedic surgery applicants have become less profound over time, making it increasingly difficult to predict a successful match based on USMLE Step scores, AOA membership, research productivity, and medical school research reputation. Future studies should evaluate differences in subjective metrics (e.g., away rotation and interview performance and letters of recommendation) by match status.
Cuando la traducción se ajusta a la legislación: los documentos plurilingües del Registro Civil
Authors: Cobos López Ingrid 
Published: April 2023

Con la apertura de fronteras de la Unión Europea y debido al movimiento actual de la población a distintos países, ya sea dentro de la UE o a cualquier otro país, los ciudadanos, como personas físicas, han de relacionarse con administraciones públicas que no hablan su idioma. Para ello, existe cierta legislación que pone a su disposición una serie de documentos plurilingües que los ciudadanos necesitan al moverse de un país a otro. Dichos documentos requieren la figura de un traductor jurídico que necesita, no solo conocer bien el lenguaje jurídico-administrativo, sino dominar también algunas ramas del Derecho. Con respecto a los documentos plurilingües, observamos que se encuentran con una gran variedad de tipologías y que algunas de ellas vienen reguladas en la legislación, por lo que el traductor debe conocer bien la legislación específica para poder traducir dichos documentos. En este trabajo pretendemos analizar la legislación internacional existente con respecto a los documentos plurilingües y su traducción.

With the opening of borders of the European Union and due to the current movement of the population to different countries, either within the EU or to any other country, citizens, as individuals, have to interact with public administrations that do not speak their languages. For this reason, there is a certain legislation that provides a series of multilingual documents that citizens need when moving from one country to another. These documents require the figure of a legal translator who needs, not only to know the legal-administrative language, but also to know about Law. With regard to multilingual documents, we see that they have a great variety of typologies and that most of them are regulated in the legislation. Therefore, the translator must know the specific legislation in order to be able to translate the mentioned documents. In this paper we intend to analyze the existing international legislation regarding multilingual documents and their translation.
Biochemical responses and volatile compounds in a peppermint chemotype grown in a controlled environment
Authors: Calgaro Laura Christina , Melisinas Valéria Aparecida Piovezan dos Santos , Gonçalves José Eduardo , Magalhães Hélida Mara 
Published: April 2023

Abstract

Peppermint is a medicinal plant with great economic importance for its protective effects against biotic and abiotic factors. This study aimed to assess the vegetative growth, biochemistry and volatile production of peppermint plants under elicitation. Nodal segments were inoculated in flasks containing Murashige and Skoog medium with one of four treatments (50 µM salicylic acid, 200 mg L− 1 chitosan, 25 µM copper sulphate, and control) and maintained for 90 days in a controlled environment. Copper treatment increased shoot growth by 43% and 68% compared with salicylic acid and chitosan, respectively. Furthermore, copper elicitation reduced the oxidation rate to only 13% and produced plants with better architecture. Salicylic acid and chitosan treatments increased the total phenolic content by 38% and 40%, respectively, compared with the control. The ferric reducing assay showed that salicylic acid and chitosan treatments increased the plant’s antioxidant activity by 82% and 96%, respectively, compared with the control. However, β-carotene, flavonoids and anthocyanins decreased with these treatments. Phenylalanine ammonia-lyase activity increased by 63% and 54% in shoots elicited with salicylic acid and chitosan, respectively. Elicitor treatment increased the number of volatile compounds detected (control = 29, salicylic acid = 32, copper = 37, chitosan = 38). Elicitation promoted significant changes in plant metabolism and chemical composition, evidenced mainly by differences in the levels of 2-hydroxy-3-(3-methyl-2-butenyl)-3-cyclopenten-1-one, d-limonene, eucalyptol, caryophyllene and l-alanine ethylamide. Monoterpene hydrocarbons were the major class in control shoots, whereas oxygenated monoterpenes were the major compounds in elicited shoots.