bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of STEM - Project Based Learning Devices to Train 4C Skills of Students
Authors: Kurniahtunnisa Kurniahtunnisa , Wowor Ester Caroline 
Published: May 2023

The study aims to describe the validity, applicability, and effectiveness of the developed learning aids. This research is a research and development (RD) research that uses the Borg and Gall model. The subjects of the wide-scale trial were 106 students of class XI at SMAN 16 Semarang. The data analysis used was descriptive percentages. The results and conclusions of this study indicate that: (1) overall learning aids are highly valid based on expert validation, and construct validity, especially for tests of critical thinking skills obtained valid and reliable results (2) learning aids are well implemented; (3) learning aids effectively improve students' critical thinking skills with 75% of students meeting the minimum completion criteria, and regarding N-gain test, there is an increase in critical thinking skills with moderate criteria. The STEM-PjBL Learning aids effectively trains the ability to think creatively, communicatively and collaboratively with high criteria.
miR-29c-3p Accelerates Mucosal Repair in Dextran Sodium Sulfateinduced
Ulcerative Colitis Mice through the KDM6B/H3K27me3/LDHA
Axis
Authors: Li Xia , Yin Chuanming , Li Jie 
Published: May 2023

Background:
Ulcerative colitis (UC) is an inflammatory intestinal disorder featured by
mucosal injury. MicroRNAs (miRNAs) play a role in the pathogenesis underlying UC.

Objectives:
This study was conducted to investigate the role of miR-29c-3p in a dextran sodium
sulfate (DSS)-induced UC mouse model and provide targets for UC treatment.

Methods:
The UC mouse model was established by DSS induction. The expression levels of miR-
29c-3p, lysine-specific demethylase 6B (KDM6B), zonula occludens-1 (ZO-1), Occludin, and lactate
dehydrogenase A (LDHA) were detected by real-time quantitative polymerase chain reaction or
Western blot assays. The mucosal injury was evaluated by disease activity index (DAI), colon length,
Hematoxylin-Eosin staining, and fluorescein isothiocyanate-glucan permeability test. The binding
between miR-29c-3p and KDM6B and the occupation of KDM6B or trimethylated H3 lysine 27
(H3K27me3) on the LDHA promoter were analyzed by the dual-luciferase and chromatinimmunoprecipitation
assays.

Results:
miR-29c-3p was downregulated while KDM6B and LDHA were upregulated in DSS mice.
miR-29c-3p overexpression reduced DAI and inflammatory cell infiltration while increasing colon
length, intestinal permeability, and levels of ZO-1 and Occludin. miR-29c-3p inhibited KDM6B
expression and increased H3K27me3 occupation on the LDHA promoter, thus inhibiting LDHA
transcription. Overexpression of KDM6B or LDHA averted the protective role of miR-29c-3p
upregulation in mucosal injury.

Conclusion:
miR-29c-3p limited KDM6B expression and increased the H3K27me3 occupation on
the LDHA promoter to enhance LDHA transcription, moderating mucosal injury and delaying UC
progression.
Targeting Neuroinflammation as Disease Modifying Approach to
Alzheimer’s Disease: Potential and Challenges
Authors: Jain Smita , Singh Ritu , Paliwal Sarvesh , Sharma Swapnil 
Published: May 2023

Abstract:
Alzheimer's disease (AD) is the most common form of dementia, having characteristic clinical
features of progressive memory loss and visuospatial, language, and cognitive impairment. In addition,
patients often suffer from comorbid depression and aggression. Aging is a major contributing
factor, though the exact pathophysiological involvement in the disease progression is debatable. Biologists
demonstrate that AD is not a result of a single pathological incident. However, an uncontrolled
myriad of events is responsible for the pathophysiological condition; hence, it is regarded as a multifaceted
disease. Pathophysiologically, AD is described by having a long preclinical stage (proteinopathy
accumulation stage), followed by a short prodromal/dementia stage (clinical symptom onset), as
evident via biomarker studies. Specific and sensitive biomarkers are needed to track disease progression
and treatment. Neuroinflammation is one of the cardinal pathophysiological events of AD that
form a positive activation loop between proteinopathy and pro-inflammatory mediators. However, the
starting point is inconclusive. The vital cells, like glia, known as brain scavenger cells, remain in harmony
between their quiescent and activated morphological states during any stimulus and help to regulate
the neuroinflammatory microenvironment. Hence, focusing on the dysfunctional microglia could
be a novel therapeutic approach to managing neuroinflammation condition in AD. This review focuses
on the translational evidence of anti-diabetic and anti-inflammatory candidates in AD management.
It also highlights the importance of the microglia activation spectrum, eicosanoid signaling, cytokine
signaling, and inflammatory mediators responsible for the neuroinflammation cascade. The repeated
failure of single-approached therapies has diverted researchers’ attention to AD-modifying approaches
and AD multimodal treatment plans. This review is an effort to brief the role of new players (like micronutrients
and nutraceutical applications) that have been reported as helpful in suppressing AD severity.
Apart from anti-diabetic candidates, various insulin-mimetic and insulin-sensitizer drugs have
also been assessed to target insulin insensitivity to mitigate AD progression. However, these possibilities
are in the investigational stage and not clinically established yet, though various AD animal models
have verified the positive outcome.
DTL is a Novel Downstream Gene of E2F1 that Promotes the Progression
of Hepatocellular Carcinoma
Authors: Dong Ruiqiang , Zhang Danhua , Han Baowei , Xu Lingxue , Zhang Danfeng , Cheng Zhenguo , Qiu Xinguang 
Published: May 2023

Background:
Hepatocellular carcinoma (HCC), one of the world's most prevalent malignancies,
accounts for 90% of primary liver cancer cases. Recent studies have shown an increased expression
of denticles E3 ubiquitin protein ligase homolog (DTL) in several different tumor types, but
its function and regulatory mechanisms remain unclear.

Aims:
This study aimed to investigate the expressions of the Cullin4 (CUL4) complex in HCC and
elucidate the roles of DTL in HCC cells.

Methods:
The relative expression of the CUL4 complex and its clinical significance were analyzed
with The Cancer Genome Atlas (TCGA) data, and the level of DTL was confirmed by immunohistochemistry.
The functions of DTL1 and upstream E2F1 were evaluated by a Western blot, MTT,
transwell, and xenograft in HCC cell lines.

Results:
The elevated mRNA expression of the CUL4 complex, including CUL4B, DDB1 (Damage
Specific DNA Binding Protein 1), and DTL, was associated with the overall survival of HCC patients.
We also found that the DTL protein was elevated in HCC tissues, and patients with highly expressed
DTL and nucleus-located DTL had a poorer survival time. DTL knockdown significantly inhibited
cancer proliferation, migration, and invasion. Further experiments showed that E2F1 was an upstream
regulatory molecule of DTL, which was bound to the promoter of DTL, promoting the expression of
DTL.

Conclusion:
The study results demonstrate that E2F1-DTL signaling promotes the growth, migration,
and invasion of HCC cells, which provides new insights and a potential biological target for future
HCC therapies
Investigation of the Apoptosis Inducing and β-catenin Silencing by Tetradentate
Schiff Base Zinc(II) Complex on the T-47D Breast Cancer Cells
Authors: Majd Mostafa Heidari , Guo Xiangyu 
Published: May 2023

Introduction:
Several mechanisms are known for the anticancer effects of cisplatin. However, its most wellknown
function involves binding to DNA and activating the DNA damage response.

Methods::
Despite its good effects, the treatment process often leads to chemoresistance and affects the mechanisms
that support cell survival, such as pathways that promote cell growth, apoptosis, DNA damage repair, and endocytosis.
For this reason, we investigated the effects of a new metal complex (tetradentate Schiff base zinc(II) complex) on
breast cancer cells (T-47D). We evaluated its effect on cytotoxicity, apoptosis, and drug resistance in comparison to
cisplatin.

Results:
The results of the MTT test showed that tetradentate Schiff base zinc(II) complex has good cytotoxicity compared
to cisplatin. The IC50 values for the [Zn(SB)]Cl2 complex and cisplatin after 72 h of exposure were equal to 42.1
and 276.1 μM, respectively. Real-time PCR assay confirmed that the [Zn(SB)]Cl2 complex activated the mitochondrial
pathway of apoptosis and increased the expression of Bak1 and caspase-3 genes significantly compared to cisplatin.
More importantly, the [Zn(SB)]Cl2 was able to reduce the expression of the β-catenin gene, which plays a role in drug
resistance, by 0.011 compared to the control.

Conclusion:
Therefore, we can hope for this new complex because, without the help of any β-catenin silencing agent,
it was able to inhibit the drug resistance in the T-47D cell line that overexpresses the β-catenin gene.
Bacterial Persister Cells: Mechanisms of Formation, Control, and Eradication
Authors: Kouhsari Ebrahim , Kaviar Vahab Hassan , Asadi Arezoo , Ahmadi Alireza , Sholeh Mohammad , Mirbalouchzehi Ali , Yaghoubi Sajad , Abdi Milad 
Published: May 2023

Abstract:
Bacterial Persister Cells (BPCs) are quiescent, slow-growing or growth-arrested phenotypic
variants of normal bacterial cells that are transiently tolerant to antibiotics. It seems that persister cells
are the main cause of the recurrence of various chronic infections. Stress response (RpoS-mediated),
Toxin-Antitoxin (TA) systems, inhibition of ATP production, Reactive Oxygen Species (ROS), efflux
pumps, bacterial SOS response, cell-to-cell communication and stringent response (ppGpp- mediated)
are the primary potential mechanisms for persistence cell formation. However, eradicating persistent
cells is challenging as the specific molecular mechanisms that initiate their formation remain fuzzy and
unknown. Here we reviewed and summarized the current understanding of how bacterial persister cells
are formed, controlled, and destroyed.
Development of a software and hardware solution to identify trends in demand for goods
Authors: Miftahova A. I. , Yangirov E. I. , Karaseva E. I. , Yangirov A. I. , Nikulina E. Yu. , Drovnikova I. G. 
Published: May 2023

Objective. The aim of the research work is to develop a software solution for identifying trends in demand for consumer goods by analyzing big data.Method. To achieve this goal, the work analyzed the current state of development of the Internet retail market in Russia, as well as the technologies and tools for analyzing big data necessary for designing a software and hardware solution. To evaluate the effectiveness of the obtained data processing model, a sample obtained from open sources is used.Result. As a result of the study, a technical solution has been developed that allows analyzing the demand for goods in a given time range based on data from open sources.Conclusion. A software component has been developed to analyze the demand for consumer goods based on order data. The resulting technical solution supports batch processing of data, and the architecture of the infrastructure component allows distributed computing. Testing the tool on a real sample showed the effectiveness of this approach to analyzing consumer demand trends.
Field studies of a thermoelectric system for thermal action on the anterior segment of the human eyeball
Authors: Evdulov O. V. , Aminova I. Yu. , Aminov G. I. , Gasanova S. G. , Surakatova P. N. 
Published: May 2023

Objective. The aim of the study is to develop the design of a thermoelectric system (TPS) for thermal action on the anterior segment of the human eyeball, as well as to conduct its field studies.Method. The study is based on the methods of thermodynamic analysis, natural and computational modeling of processes and life support facilities.Result. The design of TPP for thermal impact on the anterior segment of the human eyeball is presented. Its fullscale studies were carried out in accordance with the existing methods of thermophysical measurements. Curves of changes in the temperature of the surface of the gelatin model of the human eyeball over time during cooling and heating were obtained for the supply currents of the thermoelectric module (TEM) included in the TPP, equal to 2.5 A, 3 A, 3.5 A, A and 1 A, 1 .2 A, 1.4 A, as well as changes in the temperature of the object of influence over time when changing the cooling mode to heating and vice versa. To assess the possibilities of heat removal from the hot junctions of TEMs in the system, data were obtained on the change in their temperature over time during the cooling effect for various values of the module supply current.Conclusion. It has been established that standard modules produced by domestic firms can be used as TEMs in the system. The duration of entering the TPP mode is about 230 s. The refore, it seems appropriate to bring the TPP to the operating mode before the actual procedures are carried out. It has been established that the duration of TPP switching from cooling to heating mode and vice versa is 230 s, which requires further optimization of the design and operating modes of the device through the use of more advanced types of TEMs and the use of forced operation of thermal modules in the transient mode.
Detection of hidden defects of thermal protection of buildings and determination of some thermal properties of structural building materials by Non-destructive Thermal Imaging method
Authors: Karpov D. F. , Pavlov M. V. , Gudkov A. G. 
Published: May 2023

Objective. The purpose of the work is the practical approbation of a nondestructive thermal imaging method to identify hidden (invisible) defects and qualitative analysis of the thermal protection of a complex of construction objects in the city of Vologda, as well as the study of some physical (thermophysical) indicators (coefficients of thermal conductivity, thermal resistance (thermal insulation) and heat transfer) of structural building material in the form of silicate brick.Method. The fundamental laws of the theory of heat transfer, thermal nondestructive testing, infrared and technical diagnostics, the current domestic regulatory and technical and regulatory documentation, field experiments on real objects and laboratory samples.Result. The results of the practical determination of hidden defects in the thermal shells of the complex of civil and industrial buildings functioning in the urban environment, thermophysical properties (coefficients of thermal conductivity, thermal resistance (thermal insulation), heat transfer) of modern building materials by methods of thermal non-destructive testing are presented. The locations of identified invisible defects of thermal protection of the surveyed construction objects are determined and recommendations for their elimination are proposed. The obtained values of thermophysical characteristics for the studied structural building material are consistent with the data of current regulatory documents.Conclusion. The features of the nondestructive method of infrared thermography in determining the hidden defects of the heatshielding shells of operated buildings of various functional purposes and the complex of thermophysical parameters of modern structural building materials are shown. The demand for thermal imaging during energy and technical surveys of construction objects, their individual structural elements, life support engineering systems, as well as studies on the refinement, quantification and prediction of various thermal properties of building materials and products has been experimentally confirmed.
New types of membrane-rod and membrane-pneumatic structures and improved methods for their calculation
Authors: Kim A. Yu. , Polnikov S. V. , Amoyan M. F. 
Published: May 2023

Objective. The purpose of this study was to create new pre-fabricated membrane-rod structures and non-linear methods for their calculation.Method. A stepwise method with an iterative numerical Euler-Cauchy procedure of the third order of accuracy was applied with the use of the finite element method at the step.Result. A technique for static calculation of membrane-rod structures has been developed, taking into account nonlinear factors. A calculation algorithm has been compiled and a program for calculating such structures has been written according to it. For this program, a certificate was obtained on the computer No. 2017613415 "Program for calculating membrane-pneumatic structures, taking into account non-linear factors by the iterative method of incrementing parameters."Conclusion. It is recommended to use a one-step calculation of membrane-rod systems with five iterations per step or a multi-step calculation with five steps with one iteration per step. Mixed options are also effective.
Spatial large-span structures
Authors: Yusupov A. K. , Muselemov H. M. , Vishtalov R. I. 
Published: May 2023

Objective. This article discusses spatial large-span structures; provides features of their work; as well as layout examples. General dimensions and ways of stabilizing displacements are given.Method. The features of the work and layout of spatial large-span structures; as well as calculation formulas are given. The types of sections and nodes of conjugation of elements are presented. A technique is described that allows stabilizing the movements of largespan pavements.Result. The given structural schemes; as well as the analysis of their work; allow designing large-span spatial systems in the form of convex and concave structures that have high reliability; minimal weight; and high manufacturability in manufacturing; transportation and installation.Conclusion. The proposed rational design solutions make it possible to reduce the own weight of spatial convex and concave coatings; which have the necessary rigidity; strength and stability. The types of structural schemes; sections and junctions of elements considered in the article are widely used in the practice of design and construction.
Analysis and features of the functioning of protected automated systems of internal affairs bodies under the influence of threats of unauthorized access
Authors: Yefimov A. O. , Nasedkina V. S. , Popov A. D. , Rogozin E. A. , Romanova V. R. 
Published: May 2023

Objective. The purpose of the study is to analyze the features of the functioning of protected automated systems and develop a structural diagram of a typical ATS AS, which includes various structural elements.Method. As a fundamental method of conducting research is the method of system analysis.Result. Based on the developed typical structural diagram of the ATS AS, threats to web servers were identified in accordance with the official website of the FSTEC, and, in accordance with them, potential vulnerabilities (weak points in terms of information security) were identified; a classification of all potentially dangerous threats to the information resource of protected ATS automated systems was developed and presented.Conclusion. The materials of the article can be used to analyze and develop a methodology for quantitatively assessing the risks of information security violations of the web servers of the ATS of the Russian Federation; methods for assessing the level of security of the ATS of the Russian Federation; methods of access of regular users of the RF ATS AS to the information resource of these systems based on semantic analysis.
Principles of construction of a multifunctional intelligent electric energy meter with advanced communication and functional capabilities
Authors: Magomedov I. A. , Khidivov M. N. , Magomedov A. I. 
Published: May 2023

Objective.Development of principles for the construction of a multifunctional intelligent electric energy meter (MSE) with expanded communication and functional capabilities and the construction of MSE based on them for automated systems of commercial accounting of energy consumption (ASKUE).Method.When developing the principles of the construction of the IEE, an analysis of existing electric energy metering devices was carried out and their main disadvantages were identified, which made it possible to improve the characteristics and expand the functionality of metering devices. The operability of the main components of the IEE is confirmed by the results of mathematical and physical modeling, which showed the possibility of improving the metrological characteristics and technical parameters that determine the quantitative, qualitative and cost characteristics of the devices.Result. The principles of the construction of the IEE have been developed, which makes it possible to create IEE with expanded communication and functional capabilities for the ASUE.Conclusion. Using them in ASCUE posvolit: -to build self-adjusting mesh networks based on them, which can be used in the construction of ASCS with increased survivability; -to ensure the exchange of accounting and service information with the ASKUE over the radio channel, PCL modem and RS-485 (CAN) interface, which increases the reliability of obtaining accounting information and integrate the MISEE into existing ASKUE, because it makes it possible to select the appropriate type of specific ASKUE interface or communication interfaces "counter-ASKUE";- regulate energy consumption and independent disconnection of the consumer from the network for each subscriber unit (separately for each subscriber) in case of arrears of payments, network overload and unauthorized access to the nodes and blocks of the meter in various ways;- to improve the operating conditions of the meter when organizing electronic payments and increasing the reliability of accounting information due to contactless remote removal of information by means of communication interfaces for each subscriber or by means of an individual remote control (remote control).-to reduce the hardware costs of servicing one subscriber associated with the use of the main units and units of the meter to account for energy consumption for all subscribers, and, as a result, the low cost of electricity metering in recalculation per subscriber.