bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolutionary Genetics and Admixture in African Populations
Authors: Pfennig Aaron , Petersen Lindsay N , Kachambwa Paidamoyo , Lachance Joseph 
Published: March 2023

Abstract
As the ancestral homeland of our species, Africa contains elevated levels of genetic diversity and substantial population structure. Importantly, African genomes are heterogeneous: They contain mixtures of multiple ancestries, each of which have experienced different evolutionary histories. In this review, we view population genetics through the lens of admixture, highlighting how multiple demographic events have shaped African genomes. Each of these historical vignettes paints a recurring picture of population divergence followed by secondary contact. First, we give a brief overview of genetic variation in Africa and examine deep population structure within Africa, including the evidence of ancient introgression from archaic “ghost” populations. Second, we describe the genetic legacies of admixture events that have occurred during the past 10,000 years. This includes gene flow between different click-speaking Khoe-San populations, the stepwise spread of pastoralism from eastern to southern Africa, multiple migrations of Bantu speakers across the continent, as well as admixture from the Middle East and Europe into the Sahel region and North Africa. Furthermore, the genomic signatures of more recent admixture can be found in the Cape Peninsula and throughout the African diaspora. Third, we highlight how natural selection has shaped patterns of genetic variation across the continent, noting that gene flow provides a potent source of adaptive variation and that selective pressures vary across Africa. Finally, we explore the biomedical implications of population structure in Africa on health and disease and call for more ethically conducted studies of genetic variation in Africa.
“Picturing a Way Forward”: Strategies to Manage the Effects of COVID-19-Related Isolation on Long-Term Care Residents and Their Informal Caregivers
Authors: Boamah Sheila A , Weldrick Rachel , Yous Marie-Lee , Gao Harrison , Garnett Anna , Dal Bello-Haas Vanina , Kaasalainen Sharon 
Published: March 2023

AbstractBackground and ObjectivesStrategies to manage the coronavirus disease 2019 (COVID-19) pandemic included widespread use of physical distancing measures. These well-intended strategies adversely affected long-term care (LTC) residents’ socialization and their caregiving arrangements, leading to exacerbation of social isolation and emotional distress for both residents and their caregivers. This study aimed to understand how these measures affected informal caregivers of people living in LTC homes in Ontario. Strategies to increase socialization and promote social connection during and post-COVID-19 were also explored.Research Design and MethodsThis qualitative study used descriptive and photovoice approaches. Of the 9 potential caregivers identified, 6 participated in the study and shared their experiences and photographic reflections in virtual focus group sessions.ResultsFindings highlighted the increased social isolation experienced by people living in LTC and their caregivers during COVID-19. Caregivers reported pronounced declines in residents’ well-being and were frustrated by challenges connecting with their family members during quarantine. Attempts made by LTC homes to maintain social connections, such as window visits and video calls, did not fulfill the social needs of residents and their caregivers.Discussion and ImplicationsFindings underscore a need for better social support and resources for both LTC residents and their caregivers going forward to prevent further isolation and disengagement. Even in times of lockdown, LTC homes must implement policies, services, and programs that promote meaningful engagement for older adults and their families.
The Changing Demography of Late-Life Family Caregiving: A Research Agenda to Understand Future Care Networks for an Aging U.S. Population
Authors: Freedman Vicki A , Agree Emily M , Seltzer Judith A , Birditt Kira S , Fingerman Karen L , Friedman Esther M , Lin I-Fen , Margolis Rachel , Park Sung S , Patterson Sarah E , Polenick Courtney A , Reczek Rin , Reyes Adriana M , Truskinovsky Yulya , Wiemers Emily E , Wu Huijing , Wolf Douglas A , Wolff Jennifer L , Zarit Steven H 
Published: March 2023

Abstract
Repeated claims that a dwindling supply of potential caregivers is creating a crisis in care for the U.S. aging population have not been well-grounded in empirical research. Concerns about the supply of family care do not adequately recognize factors that may modify the availability and willingness of family and friends to provide care to older persons in need of assistance or the increasing heterogeneity of the older population. In this paper, we set forth a framework that places family caregiving in the context of older adults’ care needs, the alternatives available to them, and the outcomes of that care. We focus on care networks, rather than individuals, and discuss the demographic and social changes that may alter the formation of care networks in the future. Last, we identify research areas to prioritize in order to better support planning efforts to care for the aging U.S. population.
Changes in survival in de novo metastatic cancer in an era of new medicines
Authors: Luyendijk Marianne , Visser Otto , Blommestein Hedwig M , de Hingh Ignace H J T , Hoebers Frank J P , Jager Agnes , Sonke Gabe S , de Vries Elisabeth G E , Uyl-de Groot Carin A , Siesling Sabine 
Published: March 2023

Abstract

Background
Over the past decades, the therapeutic landscape has markedly changed for patients with metastatic solid cancer, yet few studies have evaluated its effect on population-based survival. The objective of this study was to evaluate the change in survival of patients with de novo metastatic solid cancers during the last 30 years.

Methods
For this retrospective study, data from almost 2 million patients diagnosed with a solid cancer between January 1, 1989, and December 31, 2018, were obtained from the Netherlands Cancer Registry, with follow-up until January 31, 2021. We classified patients as with or without de novo metastatic disease (M1 or M0, respectively) at diagnosis and determined the proportion with M1 disease over time. Changes in age-standardized net survival were calculated as the difference in the 1- and 5-year survival rates of patients diagnosed in 1989-1993 and 2014-2018.

Results
Different cancers showed divergent trends in the proportion of M1 disease and increases in net survival for M1 disease (approximately 0-50 percentage points at both 1 and 5 years). Patients with gastrointestinal stromal tumors saw the largest increases in 5-year survival, but we also observed substantial 5-year survival increases for patients with neuroendocrine tumors, melanoma, prostate cancer, and breast cancer.

Conclusion
Over 30 years, the survival of patients with de novo M1 disease modestly and unevenly increased among cancers. Metastatic cancer still remains a very lethal disease. Next to better treatment options, we call for better preventive measures and early detection to reduce the incidence of metastatic disease.
From critical thinking to criticality and back again
Authors: Pettersson Henri 
Published: March 2023

ABSTRACTThis paper assesses the prospects of combining the distinctive strengths of the two major educational research programs of critical thinking and critical pedagogy—or, described more accurately, overcoming their shared limitations—in a new and superior educational objective called criticality. Several recent proposals explore the possibilities of engaging in bridge-building between these camps. The plan is that the distinctive strengths of these paradigms—the logical and epistemological precision of critical thinking together with the socio-political consciousness of critical pedagogy—could complement each other, while the associated adjustments to the overall picture could also help us to address their shared shortcomings. This gives us a new and more grounded educational goal of criticality, as suggested and developed independently by a growing number of thinkers. This article joins the ongoing conversation but provides a more counterreactionary tone by striving to vindicate the traditional mainstream conception of critical thinking. I maintain that despite their admirable ambitions, the various expressions of criticality do not succeed in combining the best parts of critical thinking and critical pedagogy, since on a deep metatheoretical level these two paradigms have irreconcilable core principles. First, I argue that the depiction of the failures of critical thinking used to motivate criticality is to a large degree a straw man: in actuality, the existing conceptualization of critical thinking can already do the desired extra socio-political educational work, so there really is no pressing need for the suggested augmentations. Second, the traditional worries of indoctrination, which have followed critical pedagogy since the inception of this educational movement, still remain unresolved within criticality. The only way to address this concern successfully is to lean on critical thinking as the core of our educational theory, bringing us right back to where we started.
Genome-wide association study identifies a novel BMI1A QTL allele that confers FLC expression diversity in Arabidopsis thaliana
Authors: Wang Xiang , Miao Huaiqi , Lv Caijia , Wu Gang 
Published: March 2023

Abstract
Identification and understanding of the genetic basis of natural variations in plants are essential for comprehending their phenotypic adaptation. Here, we report a genome-wide association study (GWAS) of FLOWERING LOCUS C (FLC) expression in 727 Arabidopsis accessions. We identified B LYMPHOMA MOLONEY MURINE LEUKEMIA VIRUS INSERTION REGION 1 HOMOLOG 1A (BMI1A) as a causal gene for one of the FLC expression quantitative trait loci (QTLs). Loss of function in BMI1A increases FLC expression and delays flowering time at 16 °C significantly compared with the wild type (Col-0). BMI1A activity is required for histone H3 lysine 27 trimethylation (H3K27me3) accumulation at the FLC, MADS AFFECTING FLOWERING 4 (MAF4), and MAF5 loci at low ambient temperature. We further uncovered two BMI1A haplotypes associated with the natural variation in FLC expression and flowering time at 16 °C, and demonstrated that polymorphisms in the BMI1A promoter region are the main contributor. Different BMI1A haplotypes are strongly associated with geographical distribution, and the low ambient temperature-sensitive BMI1A variants are associated with a lower mean temperature of the driest quarter of their collection sites compared with the temperature-non-responsive variants, indicating that the natural variations in BMI1A have adaptive functions in FLC expression and flowering time regulation. Therefore, our results provide new insights into the natural variations in FLC expression and flowering time diversity in plants.
Anesthesiology Fellowships in the Military Health System: An Education Analysis of the Supply and Demand
Authors: Patzkowski Michael S , Highland Krista B , Hudson Arlene , Herrera Germaine F , Bodily Kory R , Ruffin David M , Via Darin K 
Published: March 2023

ABSTRACT

Introduction
Evidence indicates that desire for fellowship training is most influenced by personal interest, enhancement of career options, and a specific interest in an academic medicine career. The overall objective of this study is to evaluate anesthesiology fellowship interest and its potential impact on military retention and other outcomes. We hypothesized that current fellowship training accessibility is outpaced by the interest for fellowship training and that additional factors will be associated with the desire for fellowship training.

Methods
This prospective cross-sectional survey study was approved as Exempt Research by the Brooke Army Medical Center Institutional Review Board in November 2020. Participants were eligible to complete the online voluntary survey if they were active duty anesthesiologists. Anonymous surveys were administered via the Research Electronic Data Capture System from December 2020 to January 2021. Aggregated data were evaluated using univariate statistics, bivariate analyses, and a generalized linear model.

Results
Seventy-four percent of general anesthesiologists (those without fellowship training) were interested in pursuing future fellowship training versus 23% of subspecialist anesthesiologists (those currently in fellowship training or have completed fellowship training), odd ratio 9.71 (95% CI, 4.3-21.7). Of subspecialist anesthesiologists, 75% indicated serving in a nongraduate medical education (GME) leadership position (e.g., service/department chief), with 38% serving in a GME leadership position (e.g., program or associate program director). Almost half (46%) of subspecialist anesthesiologists reported being “extremely likely” to serve ≥20 years, versus 28% of general anesthesiologists.

Conclusions
There is a high demand among active duty anesthesiologists for fellowship training, which in turn, may improve military retention. The demand for fellowship training is outpaced by what the Services currently offer, including training in Trauma Anesthesiology. Leveraging this interest in subspecialty fellowship training, particularly when the skills align with combat casualty care–related requirements, would greatly benefit the Services.
Inflammation: the driver of poor outcomes among children with severe acute malnutrition?
Authors: Sturgeon Jonathan P , Njunge James M , Bourke Claire D , Gonzales Gerard Bryan , Robertson Ruairi C , Bwakura-Dangarembizi Mutsa , Berkley James A , Kelly Paul , Prendergast Andrew J 
Published: March 2023

Abstract
Severe acute malnutrition (SAM) is the most life-threatening form of undernutrition and underlies at least 10% of all deaths among children younger than 5 years in low-income countries. SAM is a complex, multisystem disease, with physiological perturbations observed in conjunction with the loss of lean mass, including structural and functional changes in many organ systems. Despite the high mortality burden, predominantly due to infections, the underlying pathogenic pathways remain poorly understood. Intestinal and systemic inflammation is heightened in children with SAM. Chronic inflammation and its consequent immunomodulation may explain the increased morbidity and mortality from infections in children with SAM, both during hospitalization and in the longer term after discharge. Recognition of the role of inflammation in SAM is critical in considering new therapeutic targets in this disease, which has not seen a transformational approach to treatment for several decades. This review highlights the central role of inflammation in the wide-ranging pathophysiology of SAM, as well as identifying potential interventions that have biological plausibility based on evidence from other inflammatory syndromes.
Accumulation of Cyanobacterial Photosystem II Containing the ‘Rogue’ D1 Subunit Is Controlled by FtsH Protease and Synthesis of the Standard D1 Protein
Authors: Masuda Takako , Bečková Martina , Turóczy Zoltán , Pilný Jan , Sobotka Roman , Trinugroho Joko P , Nixon Peter J , Prášil Ondřej , Komenda Josef 
Published: March 2023

Abstract
Unicellular diazotrophic cyanobacteria contribute significantly to the photosynthetic productivity of the ocean and the fixation of molecular nitrogen, with photosynthesis occurring during the day and nitrogen fixation during the night. In species like Crocosphaera watsonii WH8501, the decline in photosynthetic activity in the night is accompanied by the disassembly of oxygen-evolving photosystem II (PSII) complexes. Moreover, in the second half of the night phase, a small amount of rogue D1 (rD1), which is related to the standard form of the D1 subunit found in oxygen-evolving PSII, but of unknown function, accumulates but is quickly degraded at the start of the light phase. We show here that the removal of rD1 is independent of the rD1 transcript level, thylakoid redox state and trans-thylakoid pH but requires light and active protein synthesis. We also found that the maximal level of rD1 positively correlates with the maximal level of chlorophyll (Chl) biosynthesis precursors and enzymes, which suggests a possible role for rogue PSII (rPSII) in the activation of Chl biosynthesis just before or upon the onset of light, when new photosystems are synthesized. By studying strains of Synechocystis PCC 6803 expressing Crocosphaera rD1, we found that the accumulation of rD1 is controlled by the light-dependent synthesis of the standard D1 protein, which triggers the fast FtsH2-dependent degradation of rD1. Affinity purification of FLAG-tagged rD1 unequivocally demonstrated the incorporation of rD1 into a non-oxygen-evolving PSII complex, which we term rPSII. The complex lacks the extrinsic proteins stabilizing the oxygen-evolving Mn4CaO5 cluster but contains the Psb27 and Psb28-1 assembly factors.
Dilemma of immunosuppression and infection risk in systemic lupus erythematosus
Authors: He Jing , Li Zhanguo 
Published: March 2023

AbstractPatients with SLE are at high risk of various infections as evidenced by a number of studies. The main determinants of infection in SLE are disease activity, organ damage, and often inevitable medication. The molecular and cellular mechanisms underlying infection remain unclear. Impaired immunity, immunosuppressants and corticosteroids clearly increase the risk of infection, whereas some medications, such as low-dose IL-2, hydroxychloroquine and IVIG are safe in SLE patients with substantial evidence. It is important to balance the immunosuppression and infection risks in practice. This article focuses on medication-related infections in SLE and discusses the therapeutic options for the disease in clinical practice.
Neuronal-Hematopoietic Cell Fusion in Diabetic Neuropathy
Authors: Terashima Tomoya , Katagi Miwako , Ohashi Natsuko 
Published: March 2023

AbstractDiabetic neuropathy is a major complication of diabetes mellitus that occurs during the early stages of the disease. Many pathogenic mechanisms are related and induced by hyperglycemia. However, even if these factors improve, diabetic neuropathy cannot go into remission and progresses slowly. Furthermore, diabetic neuropathy often progresses even with proper glycemic control. Recently, bone marrow-derived cells (BMDCs) were reported to be involved in the pathogenesis of diabetic neuropathy. BMDCs expressing proinsulin and TNFα migrate to the dorsal root ganglion and fuse with neurons, and this neuronal-hematopoietic cell fusion induces neuronal dysfunction and apoptosis. The CD106-positive lineage–sca1+c-kit+ (LSK) stem cell fraction in the bone marrow is strongly involved in cell fusion with neurons, leading to diabetic neuropathy. Surprisingly, when CD106-positive LSK stem cells obtained from diabetic mice were transplanted into nondiabetic mice, they fused with dorsal root ganglion neurons and induced neuropathy in non-hyperglycemic normal mice. The transplanted CD106-positive LSK fraction inherited the trait even after transplantation; this “progeny effect” may explain the irreversibility of diabetic neuropathy and is a significant finding for determining the target of radical treatments and provides new directions for developing therapeutic methods for diabetic neuropathy.
Robustness and reproducibility of simple and complex synthetic logic circuit designs using a DBTL loop
Authors: Cummins Breschine , Vrana Justin , Moseley Robert C , Eramian Hamed , Deckard Anastasia , Fontanarrosa Pedro , Bryce Daniel , Weston Mark , Zheng George , Nowak Joshua , Motta Francis C , Eslami Mohammed , Johnson Kara Layne , Goldman Robert P , Myers Chris J , Johnson Tessa , Vaughn Matthew W , Gaffney Niall , Urrutia Joshua , Gopaulakrishnan Shweta , Biggers Vanessa , Higa Trissha R , Mosqueda Lorraine A , Gameiro Marcio , Gedeon Tomáš , Mischaikow Konstantin , Beal Jacob , Bartley Bryan , Mitchell Tom , Nguyen Tramy T , Roehner Nicholas , Haase Steven B 
Published: March 2023

AbstractComputational tools addressing various components of design–build–test–learn (DBTL) loops for the construction of synthetic genetic networks exist but do not generally cover the entire DBTL loop. This manuscript introduces an end-to-end sequence of tools that together form a DBTL loop called Design Assemble Round Trip (DART). DART provides rational selection and refinement of genetic parts to construct and test a circuit. Computational support for experimental process, metadata management, standardized data collection and reproducible data analysis is provided via the previously published Round Trip (RT) test–learn loop. The primary focus of this work is on the Design Assemble (DA) part of the tool chain, which improves on previous techniques by screening up to thousands of network topologies for robust performance using a novel robustness score derived from dynamical behavior based on circuit topology only. In addition, novel experimental support software is introduced for the assembly of genetic circuits. A complete design-through-analysis sequence is presented using several OR and NOR circuit designs, with and without structural redundancy, that are implemented in budding yeast. The execution of DART tested the predictions of the design tools, specifically with regard to robust and reproducible performance under different experimental conditions. The data analysis depended on a novel application of machine learning techniques to segment bimodal flow cytometry distributions. Evidence is presented that, in some cases, a more complex build may impart more robustness and reproducibility across experimental conditions.Graphical Abstract
In ovo injection of cinnamon or clove alters the physiology and growth of broilers in a hot tropical environment
Authors: Akosile Oluwaseun Ayomide , Sogunle Olajide Mark , Majekodunmi Bukola , Oke Oyegunle Emmanuel 
Published: March 2023

Abstract
A study was conducted to evaluate the influence of in ovo feeding of clove and cinnamon on broilers. The experiment used 700 broiler (Ross 308) hatching eggs that were incubated at the recommended temperature of 37.8 °C. On day 17.5 of incubation, 100 eggs were randomly assigned to each of the following seven treatments: uninjected eggs (OE), eggs injected 0.5 mL distilled water (DH), 2 mg of clove, 4 mg of clove, 2 mg of cinnamon, 4 mg of cinnamon, and 3 mg of ascorbic acid (AC). During the posthatch period, the chicks were raised for 56 days. Data on physiological parameters, growth performance, and intestinal histomorphology were collected. Results revealed that the plasma triiodothyronine (T3) of AC and CV2 chicken was higher than the others. Additionally, the plasma malondialdehyde levels of the chickens of AC, CV2, and CM2 were improved significantly (P &lt; 0.05). The initial weights of CV2 birds were comparable with AC CV4, CM4, and CM2 birds but heavier than those of OE and DW. The bodyweight gain in the CV2 group was similar to AC, CV4, and CM2 groups but heavier than OE, DW, and CM4 birds. Feed intake of OE and DW groups was similar to AC, CV2, CV4, and CM2 but higher than CM4. The feed conversion ratio of OE and DW chickens was comparable but higher than the value obtained in chickens of other treatments. The intestinal morphology of the birds did not follow a particular trend. The study concluded that the in ovo injection of 2 mg of clove improved broiler birds’ metabolic and antioxidant status at hatch. The high and low doses of clove and the low dose of cinnamon improved the performance of broiler chickens at the market age in a hot tropical environment.