bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omic analysis reveals dynamic changes of three-dimensional chromatin architecture during T cell differentiation
Authors: Zhang Ge , Li Ying , Wei Gang 
Published: July 2023

AbstractCell differentiation results in widespread changes in transcriptional programs as well as multi-level remodeling of three-dimensional genome architecture. Nonetheless, few synthetically investigate the chromatin higher-order landscapes in different T helper (Th) cells. Using RNA-Seq, ATAC-Seq and Hi-C assays, we characterize dynamic changes in chromatin organization at different levels during Naive CD4+ T cells differentiation into T helper 17 (Th17) and T helper 1 (Th1) cells. Upon differentiation, we observe decreased short-range and increased extra-long-range chromatin interactions. Although there is no apparent global switch in the A/B compartments, Th cells display the weaker compartmentalization. A portion of topologically associated domains are rearranged. Furthermore, we identify cell-type specific enhancer-promoter loops, many of which are associated with functional genes in Th cells, such as Rorc facilitating Th17 differentiation and Hif1a responding to intracellular oxygen levels in Th1. Taken together, these results uncover the general patterns of chromatin reorganization and epigenetic landscapes of gene regulation during T helper cell differentiation.
Zfp296 knockout enhances chromatin accessibility and induces a unique state of pluripotency in embryonic stem cells
Authors: Miyazaki Satsuki , Yamano Hiroyuki , Motooka Daisuke , Tashiro Fumi , Matsuura Takumi , Miyazaki Tatsushi , Miyazaki Jun-ichi 
Published: July 2023

AbstractThe Zfp296 gene encodes a zinc finger-type protein. Its expression is high in mouse embryonic stem cells (ESCs) but rapidly decreases following differentiation. Zfp296-knockout (KO) ESCs grew as flat colonies, which were reverted to rounded colonies by exogenous expression of Zfp296. KO ESCs could not form teratomas when transplanted into mice but could efficiently contribute to germline-competent chimeric mice following blastocyst injection. Transcriptome analysis revealed that Zfp296 deficiency up- and down-regulates a distinct group of genes, among which Dppa3, Otx2, and Pou3f1 were markedly downregulated. Chromatin immunoprecipitation sequencing demonstrated that ZFP296 binding is predominantly seen in the vicinity of the transcription start sites (TSSs) of a number of genes, and ZFP296 was suggested to negatively regulate transcription. Consistently, chromatin accessibility assay clearly showed that ZFP296 binding reduces the accessibility of the TSS regions of target genes. Zfp296-KO ESCs showed increased histone H3K9 di- and trimethylation. Co-immunoprecipitation analyses revealed interaction of ZFP296 with G9a and GLP. These results show that ZFP296 plays essential roles in maintaining the global epigenetic state of ESCs through multiple mechanisms including activation of Dppa3, attenuation of chromatin accessibility, and repression of H3K9 methylation, but that Zfp296-KO ESCs retain a unique state of pluripotency while lacking the teratoma-forming ability.
Single-cell analyses implicate ascites in remodeling the ecosystems of primary and metastatic tumors in ovarian cancer
Authors: Zheng Xiaocui , Wang Xinjing , Cheng Xi , Liu Zhaoyuan , Yin Yujia , Li Xiaoduan , Huang Zhihao , Wang Ziliang , Guo Wei , Ginhoux Florent , Li Ziyi , Zhang Zemin , Wang Xipeng 
Published: July 2023

AbstractOvarian cancer (OC) is an aggressive gynecological tumor usually diagnosed with widespread metastases and ascites. Here, we depicted a single-cell landscape of the OC ecosystem with five tumor-relevant sites, including omentum metastasis and malignant ascites. Our data reveal the potential roles of ascites-enriched memory T cells as a pool for tumor-infiltrating exhausted CD8+T cells and T helper 1-like cells. Moreover, tumor-enriched macrophages exhibited a preference for monocyte-derived ontogeny, whereas macrophages in ascites were more of embryonic origin. Furthermore, we characterized MAIT and dendritic cells in malignant ascites, as well as two endothelial subsets in primary tumors as predictive biomarkers for platinum-based chemotherapy response. Taken together, our study provides a global view of the female malignant ascites ecosystem and offers valuable insights for its connection with tumor tissues and paves the way for potential markers of efficacy evaluation and therapy resistance in OC.
Naive Listener Ratings of Speech Intelligibility Over the Course of Motor-Based Intervention in Children With Childhood Apraxia of Speech
Authors: Wang Emily W. , Grigos Maria I. 
Published: July 2023

Purpose:
The aim of this study was to describe changes in speech intelligibility and interrater and intrarater reliability of naive listeners' ratings of words produced by young children diagnosed with childhood apraxia of speech (CAS) over a period of motor-based intervention (dynamic temporal and tactile cueing [DTTC]).

Method:
A total of 120 naive listeners (i.e., listeners without experience listening to children with speech and/or language impairments; age range: 18–45 years) orthographically transcribed single-word productions by five children (age range: 2;6–3;11 [years;months]) across three time points over an intervention period (baseline, post-treatment, maintenance). Changes in intelligibility and interrater and intrarater reliability were examined within and across time points.

Results:

Speech intelligibility significantly increased in children with CAS over the course of treatment, and these gains were also maintained at 6 weeks posttreatment. There was poor-to-fair consistency
between
listeners (interrater reliability) and excellent consistency
within
listeners (intrarater reliability) in ratings of speech intelligibility within and across time points.

Conclusions:
Motor-based intervention increases speech intelligibility following a period of DTTC treatment. Variability among naive listeners of speech intelligibility was also present, with intrarater reliability (within listeners) yielding greater consistency than interrater reliability (between listeners). The implications for including naive listeners as raters of speech intelligibility for research and clinical purposes are discussed.
Heartbeat information prediction based on transformer model using millimetre‐wave radar
Authors: Hu Bojun , Jin Biao , Xue Hao , Zhang Zhenkai , Xu Zhaoyang , Zhu Xiaohua 
Published: July 2023

AbstractMillimetre‐wave radar offers high ranging accuracy and can capture subtle vibration information of the human heart. This study proposes a heartbeat prediction method based on the transformer model using millimetre‐wave radar. Firstly, the millimetre‐wave radar was used to collect the heartbeat data and conduct normalisation processing. Secondly, a position coding was introduced to assign sine or cosine variables to input data and extract their relative position relationship. Subsequently, the transformer encoder was adopted to allocate attention to input data through the multi‐head attention mechanism, using a mask layer before the decoding layer to prevent the leakage of future information. Finally, we employ the fully connected layer was employed in the linear decoder for regression and output the predicted results. Our experimental results demonstrate that the proposed transformer model achieves nearly 30% higher prediction accuracy than traditional long short‐term memory models while improving both the prediction accuracy and convergence rate. The proposed method has great potential in predicting the heartbeat state of elderly and sick patients.
Fragen an das Dr.-Sommer-Team
Authors: Döring Nicola 
Published: July 2023

Zusammenfassung
Einleitung Jugendzeitschriften wie „Bravo“ und „Bravo Girl!“ sind auch im Digitalzeitalter noch bedeutsame sexuelle Sozialisationsinstanzen. Insbesondere die Beratung durch das Dr.-Sommer-Team erfreut sich großer Bekanntheit und Beliebtheit unter Jugendlichen in Deutschland.
Forschungsziele Ziel der vorliegenden Studie war es herauszuarbeiten, welche Merkmale die Ratsuchenden haben, die in der Dr.-Sommer-Rubrik von „Bravo“ und „Bravo Girl!“ sichtbar werden (Forschungsfrage 1), welche Themen ihre Fragen ansprechen (F2), inwiefern es in den Fragen und Antworten um zentrale Risiken oder Chancen der Sexualität (F3) sowie um sexualbezogenen Mediengebrauch geht (F4).
Methoden Im Rahmen einer quantitativen Medieninhaltsanalyse wurden alle Fragen an das Dr.-Sommer-Team samt zugehörigen Antworten ausgewertet, die in den Jahren 2018 und 2019 in der 14-täglich erscheinenden „Bravo“ (n = 306) und in der monatlich erscheinende „Bravo Girl!“ (n = 121) veröffentlicht wurden (N = 427). Die Datenerhebung erfolgte mittels eines reliabilitätsgeprüften Codebuchs durch einen geschulten Codierer. Die Datenanalyse beinhaltete deskriptiv- und inferenzstatistische Auswertungen sowie qualitative Interpretationen.
Ergebnisse Es zeigte sich, dass als Ratsuchende vor allem 14-jährige Mädchen mit weißer Hautfarbe und normattraktivem Körper sichtbar waren (F1). Bei den angesprochenen Themen waren Körper, Emotionen, Beziehungen und sexuelle Techniken vorrangig vertreten (F2). Risiken (z. B. ungeplante Schwangerschaft) und Chancen (z. B. sexuelles Vergnügen) wurden in den Fragen und Antworten ausgewogen thematisiert, wobei in der Jugendzeitschrift „Bravo“ die Lust beim Partnersex als Thema signifikant sichtbarer war als in der Mädchenzeitschrift „Bravo Girl!“ (F3). Medienthemen waren in 9 % der Fragen präsent (F4).
Schlussfolgerung Die Ergebnisse stehen im Einklang mit früheren Inhaltsanalysen der Sexualaufklärung in Jugendzeitschriften im Allgemeinen und in der „Bravo“ im Besonderen. Mediennutzungs- und Wirkungsstudien zur Sexualberatung in Jugendzeitschriften fehlen indessen weitgehend.
Integrierte Behandlung von Patient*innen mit somatischer
Belastungsstörung in Hausarztpraxen – Eine qualitative,
patient*innenbeteiligende Evaluation einer randomisierten
Machbarkeitsstudie
Authors: Tönnies Justus , Graue Leike , Ayoub-Schreifeldt Marayah , Wensing Michel , Szecsenyi Joachim , Friederich Hans-Christoph , Haun Markus W. 
Published: July 2023

Zusammenfassung
Ziel der Studie Die VISION-Intervention ist eine manualisierte
Kurzzeitbehandlung für Menschen mit somatischer
Belastungsstörung, die von Psychotherapeut*innen über
Videokonsultationen durchgeführt wird und direkt in die Hausarztpraxis
integriert ist. Als innovativer technologiebasierter Ansatz wurde die
Intervention zuletzt in einer randomisierten Machbarkeitsstudie erprobt. Im Zuge
der hier vorgestellten qualitativen Begleitstudie sollte durch Einbezug der in
die Machbarkeitsstudie eingeschlossenen Patient*innen die Intervention
evaluiert und aus der Perspektive der Nutzer*innen weiterentwickelt
werden.
Methodik Es wurden insgesamt N=10 in die Interventionsgruppe
eingeschlossene Patient*innen in drei leitfadengestützten
Fokusgruppendiskussionen dazu befragt, wie sie (1) die Intervention hinsichtlich
Akzeptanz und Wirksamkeit einschätzen und (2) welche Anpassungen aus der
Nutzer*innenperspektive zur weiteren Optimierung der Intervention
erforderlich sind. Die Auswertung erfolgte softwaregestützt mittels
qualitativer Inhaltsanalyse in MAXQDA Plus 2022.
Ergebnisse Die Befragten berichteten von ihren anfänglichen
Vorbehalten bezüglich der Versorgung per Videokonsultation. Nach einer
gewissen Zeit der Gewöhnung wurde die Intervention in einem hohen
Maße akzeptiert. Zu den wichtigsten Verbesserungsvorschlägen
gehörten ein stärkerer Fokus auf die Unterstützung der
Patient*innen bei der Suche nach einer Anschlusspsychotherapie (falls
indiziert), flexiblere Rahmenbedingungen (z. B. Ort der
Videokonsultation) sowie eine noch stärkere Integration der Behandlung
in die hausärztliche Versorgung.
Diskussion und Schlussfolgerung Die Studie liefert systematisch
ausgearbeitete Erkenntnisse zu Behandlungserfahrungen von Menschen mit
somatischer Belastungsstörung. Das Modell scheint geeignet zu sein zur
Behandlung von Menschen mit somatischer Belastungsstörung. Wichtige
Verbesserungsvorschläge sollten vor der Umsetzung in der Zukunft
gründlich abgewogen werden. Dabei sollten Betroffene weiterhin
einbezogen werden.
Ähnliche Weisheitsstrategien für unterschiedliche
Lebensprobleme
Authors: Meier-Credner Anne , Eberl-Kollmeier Mareike , Muschalla Beate 
Published: July 2023

Zusammenfassung
Ziel Weisheit kann verstanden werden als Fähigkeit,
unlösbare Probleme zu lösen. Weisen Menschen gelingt es besser,
verschiedene schwierige Lebenssituationen zu bewältigen. Dabei helfen
ihnen Weisheitsstrategien. Fraglich ist, ob in verschiedenen schwierigen
Lebenssituationen die gleichen oder verschiedene Weisheitsstrategien
benötigt werden. Diese Studie geht deshalb der Frage nach, ob Menschen
die gleichen oder unterschiedliche Weisheitsstrategien für sinnvoll
halten, wenn sie mit zwei verschiedenen Lebensproblemen konfrontiert werden.
Methodik Insgesamt 416 Personen (40,4 Jahre alt) lasen jeweils zwei
Vignetten, eine zu einer beruflichen Problemsituation und eine zu einer privaten
Problemsituation. Für jede Situation schätzten sie ein, wie
stark sie den angebotenen Weisheitsstrategien, bezogen auf die jeweilige
Situation, zustimmten. Die Weisheitsstrategien wurden mittels
Selbstbeurteilungsfragebogen erfasst.
Ergebnisse Die Befragten stimmten den Weisheitsstrategien für
beide Situationen ähnlich stark zu. Für beide Situationen
stimmten sie am stärksten der Bedeutung von Fakten- und
Problemlösewissen, Emotionswahrnehmung und -akzeptanz, Wertrelativismus
sowie Problem- und Anspruchsrelativierung zu. Die Reihenfolge der
übrigen acht Weisheitsstrategien ist für beide Situationen
dieselbe.
Diskussion und Schlussfolgerung Menschen tendieren dazu, allgemeinen
Weisheitsstrategien zuzustimmen, ungeachtet der situationsspezifischen
Hinweisreize. Weisheitsstrategien werden über verschiedene
Lebenssituationen hinweg für sinnvoll erachtet. Das ist bedeutsam
für die Gestaltung von Präventions- und
Interventionsmaßnahmen zur Förderung psychischer Resilienz.
Latency of epileptic and psychogenic nonepileptic seizures
Authors: Ozkan Hulya , Turksever Meliha , Guldiken Baburhan , Sut Necdet 
Published: July 2023

Abstract
Background Due to their semiological similarities, psychogenic nonepileptic seizures (PNESs) can occasionally hardly be differentiated from epileptic seizures (ESs), and long-term video-electroencephalographic monitoring (VEM) is needed for the differential diagnosis.
Objective To investigate the time of the first clinical event and its distribution on the days of VEM in ES and PNES patients.
Methods In total, a consecutive series of 48 PNES and 51 ES patients matched for gender and age were retrospectively and consecutively evaluated. The time distribution of the seizures during the day was noted. Seizure latency was determined as the time in hours from the start of the video-electroencephalographic recording to the first clinical event.
Results The seizure latency was significantly shorter in PNES patients compared to ES patients (p < 0.001). Seventy-two percent of PNES patients and 49.1% of ES patients had their first seizure in the 24 hours of video-EEG recording (p = 0.023). Recording longer than 48 hours was required for 12.5% of PNES patients and 37.3% of ES patients (p = 0.006). While ESs were almost evenly distributed throughout the day, most PNESs occurred during the evening hours (p = 0.011).
Conclusion We observed that the PNESs appeared earlier than the ESs in the VEM and were concentrated during daylight hours. Although not strictly reliable, seizure latency can contribute to the differential diagnosis of ES and PNES.
The Impact of Tinnitus Severity on Work Functioning among U.S. Military Veterans with Tinnitus
Authors: Coco Laura , Hooker Elizabeth R. , Gilbert Tess A. , Harker Graham R. , Clark Khaya D. , Reavis Kelly M. , Henry James A. , Zaugg Tara L. , Carlson Kathleen F. 
Published: July 2023

AbstractTinnitus is highly prevalent among military Veterans. Severe tinnitus can be associated with negative impacts on daily life. Veterans with severe tinnitus may also have greater difficulties in functional roles, including work. However, few studies have explicitly explored this relationship. Traumatic brain injury (TBI), also prevalent among Veterans, is associated with tinnitus and can additionally impair work functioning. This quantitative investigation used a population-based survey to assess the relationship between tinnitus severity, measured using the Tinnitus Functional Index (TFI), and the impact of tinnitus on work, measured using a composite score from the Tinnitus History Questionnaire, among a stratified random sample of VA healthcare-using Veterans diagnosed with tinnitus, with and without comorbid TBI. Analyses were weighted to account for sampling design and Veteran non-response; multiple imputation was used to account for missing data. Results indicated that for every 1-point increase in TFI score, there was an average 8% increase in the odds of reporting a high level of impact on work functioning (OR: 1.08; 95% CI: 1.06, 1.11). Veterans with a comorbid TBI diagnosis, compared with those without, were more likely to have high tinnitus-related impact on work functioning (OR: 2.69, 95% CI: 1.85, 3.91), but the relationship between tinnitus severity and the impact of tinnitus on work functioning did not differ by TBI status. These data can help researchers and clinicians understand complex symptoms experienced by Veterans with tinnitus, with and without TBI, supporting the improved provision of clinical services to these patients.
Complications and Long-Term Outcomes after Combined Tibial Plateau Leveling Osteotomy and Tibial Tuberosity Transposition for Treatment of Concurrent Cranial Cruciate Ligament Rupture and Grade III or IV Medial Patellar Luxation
Authors: Redolfi Giovanna , Grand Jean-Guillaume 
Published: July 2023

Abstract
Objectives This study aims to report complications and long-term outcomes of combined tibial plateau leveling osteotomy and tibial tuberosity transposition (TPLO-TTT) for treatment of concurrent cranial cruciate ligament rupture and medial patellar luxation (MPL).
Study Design This is a retrospective study. Dogs that underwent TPLO-TTT for treatment of combined cranial cruciate ligament rupture and grade III or IV MPL were included. Signalment, fixation method, and complications were recorded. Long-term outcome assessment (minimum 1 year postoperatively) was performed through clinical and radiographic reexamination at the authors' institution.
Results Twenty-four stifles (22 dogs) were included. Twenty-one stifles had a grade III MPL, and 3 stifles had grade IV MPL. Four major complications consisting of surgical site infection (n = 3) and recurrent grade II MPL (n = 1) were observed. Dogs with surgical site infections were successfully treated with implant removal and oral antibiotics. No additional surgery was performed for the patient with recurrent MPL as no signs of lameness were observed. Minor complications occurred in five cases. At long-term evaluation (median: 27 months; range: 12–67 months), 21/22 dogs were clinically sound and 23/24 stifles had a complete resolution of MPL.
Conclusion TPLO-TTT can be considered as an effective surgical option to treat patients with concurrent cranial cruciate ligament rupture and MPL with a relatively low major complication rate. Owners should be warned of the potential need for implant removal.
Infarctive Apoplexy of Previously Healthy Pituitary Glands: A Small Case Series and Literature Review
Authors: Funari Abigail , Jeong Seth S. , Pecorari Isabella L. , Flaquer Isabella , Anderson Cassidy L. , Agarwal Vijay 
Published: July 2023

Abstract
Introduction We present two cases of suspected pituitary apoplexy found instead to be infarcted pituitary glands without histopathologic evidence of neoplastic cells, likely resulting from spontaneous infarction of previously healthy pituitary glands.
Case Presentations The first case is a 55-year-old man who presented with a pulsating headache, nausea, and several months of decreased libido, polyuria, and polydipsia. Magnetic resonance imaging (MRI) revealed a rim-enhancing sellar/suprasellar mass with evidence of recent hemorrhage on the right. Testosterone, follicle-stimulating hormone, and luteinizing hormone levels were suppressed. Analysis of the resected specimen showed fibrocollagenous tissue with evidence of old hemorrhage and microscopic focus of necrotic tissue. The second case is a 56-year-old man who presented with a throbbing headache, associated nausea, and 6 weeks of polyuria and polydipsia. Testosterone levels were found to be low, and 8-hour water deprivation test showed evidence for partial diabetes insipidus. MRI revealed a mass on the right side of the pituitary gland, with evidence of likely hemorrhage on the left. Analysis of the resected specimen showed necrotic tissue without neoplastic cells.
Conclusion When evaluating small pituitary lesions in patients presenting with indolent onset of pituitary insufficiency, there should be a high degree of suspicion for an infarcted pituitary gland.
Inverse Association of Lipoprotein(a) on Long-Term Bleeding Risk in Patients with Coronary Heart Disease: Insight from a Multicenter Cohort in Asia
Authors: Wang Peizhi , Yuan Deshan , Zhao Xueyan , Zhu Pei , Guo Xiaogang , Jiang Lin , Xu Na , Wang Zhifang , Liu Ru , Wang Qingsheng , Chen Yan , Zhang Yongzhen , Xu Jingjing , Liu Zhenyu , Song Ying , Zhang Zheng , Yao Yi , Feng Yingqing , Tang Xiaofang , Wang Xiaozeng , Gao Runlin , Han Yaling , Yuan Jinqing 
Published: July 2023

Background Lipoprotein(a), or Lp(a), has been recognized as a strong risk factor for atherosclerotic cardiovascular disease. However, the relationship between Lp(a) and bleeding remains indistinct, especially in the secondary prevention population of coronary artery disease (CAD). This investigation aimed to evaluate the association of Lp(a) with long-term bleeding among patients with CAD.
Methods Based on a prospective multicenter cohort of patients with CAD consecutively enrolled from January 2015 to May 2019 in China, the current analysis included 16,150 participants. Thus, according to Lp(a) quintiles, all subjects were divided into five groups. The primary endpoint was bleeding at 2-year follow-up, and the secondary endpoint was major bleeding at 2-year follow-up.
Results A total of 2,747 (17.0%) bleeding and 525 (3.3%) major bleeding were recorded during a median follow-up of 2.0 years. Kaplan–Meier survival analysis showed the highest bleeding incidence in Lp(a) quintile 1, compared with patients in Lp(a) quintiles 2 to 5 (p < 0.001), while the incidence of major bleeding seemed similar between the two groups. Moreover, restricted cubic spline analysis suggested that there was an L-shaped association between Lp(a) and 2-year bleeding after adjustment for potential confounding factors, whereas there was no significant association between Lp(a) and 2-year major bleeding.
Conclusion There was an inverse and L-shaped association of Lp(a) with bleeding at 2-year follow-up in patients with CAD. More attention and effort should be made to increase the clinician awareness of Lp(a)'s role, as a novel marker for bleeding risk to better guide shared-decision making in clinical practice.