bluuebunny/crossref_metadata_2025_split huggingface dataset:
LC/MS based untargeted lipidomics reveals lipid signatures of Non-Puerperal Mastitis
Authors: Chen Xiaoxiao , Shao Shijun , Wu Xueqing , Feng Jiamei , Qu Wenchao , Gao Qingqian , Sun Jiaye , Wan Hua 
Published: April 2023

Abstract
Background: Non-Puerperal Mastitis (NPM) is a type of disease that occurs during non-lactation accompanied by redness, swelling, heat and pain which is easily confused with breast cancer. The etiology of NPM is not yet clear. There is growing evidence from research showing that lipid metabolism may play an important role in NPM. This study aimed to use lipidomics to provide evidence for the etiology and potential therapeutic approaches for NPM.
Method: Liquid chromatography mass spectrometry (LC/MS)-based untargeted lipidomics analysis combined with multivariate statistics were performed on investigating the NPM lipid change in breast tissue.
Results: The results revealed significant differences in lipidomics profiles and a total of 16 subclasses with 14,012 different lipids were identified in positive and negative ion modes. Among these lipids, Triradylcglycerols (TAGs), Glycerophosphoethanolamines (PEs) and Glycerophosphoglycerophosphoglycerols (CLs)are top three lipid components between the NPM and control groups. Subsequently, 35 significantly different lipid metabolites were screened as potential biomarkers, such as 15(S)-Hydroxyeicosatrienoic acid, Lipoxin A4 (LXA4), Lipoxin B4 (LXB4) and LysoPC(16:0), and they showed good diagnostic performance. Detailed pathway analysis revealed that these dysregulated lipids are closely related to arachidonic acid metabolism, glycerophospholipid metabolism.
Conclusions: This study showed that NPM patients' breast tissue exhibited altered lipid groups. In addition, the lipid metabolites screened and the pathways identified in this study may be potential targets for future studies of the pathogenesis of NPM.
Trial registration: Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine (Number: 2019-702-57; Date: July 2019).
TRIM32 Reduced the Recruitment of Innate Immune Cells and the Killing Capacity of Listeria monocytogenes by Inhibiting Secretion of Chemokines
Authors: OuYang Xuan , Liu Peng , Zheng Yuling , Jiang Hua , Lv Qingyu , Huang Wenhua , Hao Huaijie , Pian Yaya , Kong Decong , Jiang Yongqiang 
Published: April 2023

Abstract
Listeria monocytogenes (Lm) is a facultative, intracellular Gram-positive pathogenic bacterium that causes sepsis, a condition characterized by persistent excessive inflammation and organ dysfunction. However, the pathogenesis of Lm-induced sepsis is unknown. In this research, we discovered that TRIM32 is required for innate immune regulation during Lm infection. Trim32 deficiency remarkably reduced bacteremia and proinflammatory cytokine secretion in mice with severe Lm infection, preventing sepsis. Trim32-/- mice had a lower bacterial burden after Lm infection and survived significantly longer than wild-type (WT) mice, as well as lower serum levels of inflammatory cytokines TNF-α, IL-6, IL-18, IL-12p70, IFN-β, and IFN-γ at 1 day post infection (dpi) compared to WT mice. On the other hand, the chemokines CXCL1, CCL-2, CCL-7, and CCL-5 were increased at 3 dpi in Trim32-/- mice compared to WT mice, implying increased recruitment of neutrophils and macrophages to clear Lm. Furthermore, Trim32-/- mice also produced more macrophage-associated iNOS. Collectively, our findings suggest that TRIM32 reduces innate immune cells recruitment and Lm killing ability by inhibiting chemokines secretion.
Tunable random laser in capillary with Nile red solution and TiO2 nanoparticles
Authors: Fang Yuwei , Hu Jigang , Huang Chan 
Published: April 2023

Abstract
We report a tunable random laser in Nile red (NR) solution with TiO2 nanoparticles. The good lasing characteristics of NR and random scattering environment provided by TiO2 bring excellent random laser emission. We analyze the influence of TiO2 number density to the threshold of random laser and draw the output energy stability via pump time. Furthermore, considering the solvatochromism of NR, the solvent is substituted by ether and acetone while the optimal number densities of TiO2 in each system are obtained respectively. In addition, we use sulfuric acid to change the polarity of the sample and obtain the random laser spectra that change continuously with the polarity of the solution. This combination of NR and TiO2 could shed new light upon the generation and tuning of random laser.
Episode I. Exploring Streptococcus pneumoniae capsular typing through MALDI-TOF mass spectrometry and machine-learning algorithms in Argentina: Identifying prevalent NON PCV13 serotypes alongside PCV13 serotypes.
Authors: Zintgraff Jonathan , Rocca Florencia , Eluchans Nahuel Sánchez , Irazu Lucía , Moscoloni Maria Alicia , Lara Claudia , Santos Mauricio 
Published: April 2023

Abstract
Laboratory surveillance of Streptococcus pneumoniae serotypes is crucial for the successful implementation of vaccines to prevent invasive pneumococcal diseases. The reference method of serotyping is the Quellung reaction, which is labor-intensive and expensive. In the last few years, the introduction of MALDI-TOF MS into the microbiology laboratory has been revolutionary. In brief, this new technology compares protein profiles by generating spectra based on the m/z ratio. We evaluated the performance of MALDI-TOF MS for typing serotypes of S. pneumoniae isolates included in the PCV13 vaccine using a machine learning approach. We challenged our classification algorithms with a total of new 215 isolates of S. pneumoniae from Argentinian nationwide surveillance. Our work involved a thorough evaluation and comparison of multiple machine learning models. Through this evaluation, we achieved high accuracy values, indicating the effectiveness of the approach taken in solving the problem. Additionally, we attained high kappa index values, indicating that the predictions made by the models were highly accurate and reliable. We also carefully considered the strengths and limitations of each model. As such, our results are highly valuable and have important implications for future research and applications.
Determinants of Pregnancy Outcomes after Assisted Reproductive Therapy: A Sample from the West Bank, Palestine
Authors: Arafat Hasan , Qamhia Diaeddin , Maqboul Husam , Al-Khayyat Abdulsalam 
Published: April 2023

Abstract
Objective: Infertility is a public health issue with a huge impact on the well-being of affected couples. Despite the traditional valuing of fertility within the Palestinian society, no studies were conducted to detect the determinants of pregnancy outcome in Palestine. This paper aims at detecting the characteristics and pregnancy outcome after assisted reproductive technology (ART) of a sample of the Palestinian society.
Materials & Methods: A retrospective observational study was carried out at Razan Medical Center for Infertility. Subjects were assigned 2 categories based on the type of infertility, 7 different categories according to the cause of infertility, and 3 categories for the modality of treatment. Age at marriage, age at presentation, duration of infertility, in addition to regularity of menstruation were also studied. Biochemical pregnancy was considered the endpoint for the purpose of analysis.
Results: We reviewed the files of 459 subjects diagnosed with infertility. Of the studied subjects, 79.74% had primary infertility, while 20.26% had secondary. According to diagnosis, 28.85% were found to be infertile due to anovulation, 2.86% due to endometriosis, 16.74% attributed to male factor, 3.3% had tubal damage, while 34.80% had no explainable cause. 13.43% of cases were multifactorial. Four biochemical markers were assessed in our study, thyroid-stimulating hormone (TSH) (x̄=2.32±2.46), leutinizing hormone (LH) (x̄=6.71±4.90), follicle-stimulating hormone (FSH) (x̄=6.59±6.11), and human prolactin (hPRL) (x̄=41.88±6.50). The menstrual cycle was regular in 70.58% of subjects, in contrast to 29.41% with irregular cycles. Female mean age at presentation 22.76±5.58, while their mean age of marriage was 22.76±4. Mean duration of infertility was 3.97±6.87. Patients were treated via 3 modalities, ovarian stimulation (2.2%), intrauterine insemination (IUI) (29.58%), and intracytoplasmic sperm injection (ICSI) (68.21%), with a rate of success of 51.85%. Of the studied factors, only diagnosis, FSH, and modality of treatment had a significant impact on the outcome, with p-values of 0.040, 0.003, and <.0.001, respectively.
Conclusions: there is a strong relationship between diagnosis prior to intervention, level of FSH and treatment modality on successful outcome of ART. ICSI was found to be superior to other modalities of treatment. We recommend starting with ICSI to save patient time & effort.
Changes of Extreme Climate Indices on the Mongolian Plateau during 1981-2020 Based on ERA5 Reanalysis
Authors: Zhu Guoying , Zhao Xinyi 
Published: April 2023

Abstract
The Mongolian Plateau (MP) is susceptible to extreme climate events due to its unique geographical location and socio-economic conditions. The focus of this study was to analyze the changes in extreme climate indices on the MP based on the ERA5 reanalysis dataset, as well as to investigate the relationship between these indices and atmospheric circulation indices. 1) Results indicated that the inter-annual variation trends of Summer days, Warm days, and Warm nights have significantly increased across the entire MP, with the central region experiencing a higher probability of extreme hot events. The inter-decadal variation of extreme hot indices rose the fastest from period 2 (1991-2000) to period 3 (2001-2010). 2) Results showed a substantial decrease in extreme cold events throughout the study period, with the most rapid decrease observed from period 1 (1981-1990) to period 2 (1991-2000), except for Frost days. 3) There was a decrease in extreme wet indices from 1981-2020. The Simple Precipitation Intensity Index (SDII) and Total precipitation in wet days (PRCPTOT) exhibited a significant descending trend in the northern and northeastern MP. Decadal variations showed a decrease in extreme precipitation, with the most significant decline observed between period 2 (1991-2000) to period 3 (2001-2010). 4) Extreme precipitation indices indicate a negative correlation with the Atlantic Meridional Mode (AMM). The Arctic Oscillation (AO) displayed a negative correlation with extreme cold indices, whereas the Pacific Decadal Oscillation (PDO) demonstrated a negative correlation with extreme hot indices.
Nutrient Decline and Metal Pollution Since the Second Industrial Revolution: The Red Sea
Authors: Cai Chunzhi , Delgado-Huertas Antonio , Agusti Susana 
Published: April 2023

Abstract
Reconstructing element concentrations in sediment cores helps identify historical natural or human-induced environmental changes. This study investigates variations in 15 element concentrations in Red Sea (a hot spot for biodiversity) sediment cores over the past five centuries. The findings reveal a significant change in sediment element accumulation rates (EAR) following the Second Industrial Revolution (~1870) and the opening of the Suez Canal. In the North, where industrial activities become prevalent, we observe an increase in the mean EAR of Fe, Cd, V, Zn, Cu, and Cr from 4.56% to 17.6%, with positive slope change rates ranging from 332% (Fe) to 1003% (Cu). Conversely, in the South, we observe a decline in the mean EAR of Mg, total N, total organic C, and Ca from -8.5% to -17.8%, with negative slope change rates ranging from -83% (Ca) to -13980% (Mg). The results reveal the increasing accumulation of trace metals from human activities in recent decades in the North, whereas the South is experiencing a decline in nutrient input from the Indian Ocean associated with ocean warming. These two challenges may be synergistic and have a detrimental effect on the Red Sea ecosystems.
Network Pharmacology Approach for the Mechanism of Traditional Chinese Medicine in Acne
Authors: WANG Yunong , ·NIE Jing , ·WANG Ying , ·ZHANG Fengchuan 
Published: April 2023

Abstract
Objective To explore the molecular mechanism of traditional Chinese medicine (TCM) in the treatment of acne based on network pharmacology.
Methods The main active components of TCM were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP）and Encyclopedia of Traditional Chinese Medicine (ETCM) database and the target was predicted by Swiss Target Prediction website. At the same time, the related targets of ance were screened from Gene Card database, Disgent database, and Therapeutic Target Database (TTD)database. The protein-protein interaction network was constructed using String website and Cytoscape 3.9.1 software after mapping the component target and disease target. Molecular docking technology was used to match potential active components with key targets. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were analyzed by Metascape website. Cytoscape3.9.1 software was used to construct the herb-component-target-pathway network.
Results 207 active ingredients and 1722 potential targets were screened from TCM, among which 70 core targets and 20 pathways were closely related to the treatment of acne. Molecular docking results showed that the active ingredients and their key targets had good binding activity.
Conclusion TCM has biological support in treating acne. Different proteins have synergistic effects, in which SRC, MAPK1, STAT3, RELA, RXRA, EP300, NFKB1, and NFKBIA, etc. play a key role. Biological processes are mainly involved in hormone synthesis, endocrine regulation, angiogenesis, cell signal transduction, inflammation, etc. These targets are mainly concentrated in multiple signaling pathways such as MAPK and Rap1 signal pathways, which play key roles in anti-inflammatory, anti-skin keratosis, regulation of cell adhesion, repair of the skin barrier, and so on.
The IMPACT Score: A new score to predict the risk of early mortality in cardiogenic shock patients treated with venoarterial extracorporeal membrane oxygenation
Authors: Simon VEYRET , Charles VIDAL , Leandre GIRARD , Laurence DANGERS , Baptiste EMOND , Berenice PUECH , Jerome ALLYN , Nicolas ALLOU , Eric BRAUNBERGER 
Published: April 2023

Abstract
Purpose :
Venoarterial-ECMO (VA-ECMO) is indicated in the management of refractory cardiogenic shock. There is currently no risk factor for early mortality, i.e. occurring within 72 hours of implantation. Highlighting them could avoid unnecessary recourse to this costly and heavy-care technique. The objective of this study is to determine the risk factors associated with early mortality in patients implanted with VA-ECMO and to construct a prognostic score.
Methods:
This single-center retrospective study included all patients treated with VA-ECMO at Felix Guyon Hospital in Reunion Island over the period 2014-2020. The pre-implantation risk factors for early mortality were sought by logistic regression and integrated into the IMPACT score.
Results:
A total of 165 patients were included in the study. Early mortality (<72 hours) was 20% (33 patients). Four pre-implantation risk factors were identified and integrated into the IMPACT score: lactate levels > 8mmol/L (Odds ratio (OR) = 4.2; p = 0.007), platelet count < 100G/L (OR = 4.9; p = 0.022), renal replacement therapy (OR = 7.1; p = 0.001), and cardiac arrest (OR = 3.2; p = 0.036). The IMPACT score had an AUC of 0.80 [95% Confidence Interval 0.73-0.88].
Conclusion:
The IMPACT score can help the physicians to identify patients who do not expect any benefit from VA-ECMO. Its validity should be tested in other populations treated with VA-ECMO.
Studying Baikal region sand properties
Authors: Yakovleva A. A. , Guseva E. A. , Konstantinova M. V. 
Published: April 2023

The study is aimed at a more detailed determination of the thermal properties of Baikal region sands and establishment of their dependence on geological and mineralogical characteristics in order to assess the possibility of using this raw material in the food industry. The objects of the study are sands sampled on the southwestern coast of Lake Baikal. The subject of the study is the mineralogical composition of sand, its grain size composition and heat capacity. The mineral composition of sand samples and their crystal structure were studied using the method of X-ray phase analysis. A standard sieve analysis method was used for granulometric estimation of sands. The average heat capacity of sand samples was determined by the mixing method. Based on the analysis of the obtained X-ray diffraction patterns, the mineralogical composition of the studied samples was determined. It was found that quartz predominates in their composition since its percentage in samples amounts to 40–60 %. The proportion of such minerals as albite and anorthoclase is 15–30 % in different samples. Less than 5 % in each sample accounts for dickite and indialite. The presence of the mineral of anthophyllite has been found only in one sample in the amount of about 9 %. The results of granulometric composition determination show that a fraction, which accounts for 70–90 %, can be isolated in most samples. The sample from the town of Slyudyanka was the only one where the fractions of different sizes were present in approximately equal amounts. For this sample, the dependence of heat capacity on the grain size was determined. In other samples, the difference in certain heat capacity values correlated well with their mineralogical composition. The results of the conducted studies and their analysis allow to conclude that Slyudyanka and Baikalsk sands are the most attractive sands in terms of high heat capacity. The sand with the grain size from 0.125 to 0.25 mm is found to be the most suitable for the use as a thermal storage medium. This fraction is predominant in the sand of the town of Baikalsk.
Keterkaitan Persentase Lemak Tubuh, Asupan Serat, dan Rasio Lingkar Pinggang Pinggul dengan Tekanan Darah Lansia
Authors: Kusuma Hapsari Sulistya , Widanti Mirza Nursania , Bening Salsa , Bintanah Sufiati 
Published: April 2023

This study aims to determine the relationship between the percentage of body fat, fiber intake and hip waist circumference ratio with blood pressure of elderly. This type of research used an analytic observational study with a cross sectional approach. It was conducted on 46 elderly people selected by purposive sampling method. The data collected includes blood pressure, measurements using a sphygmomanometer. Measurement of body fat percentage using the BIA instrument. Measurement of fiber intake with a 2x24 hour food recall instrument. As well as measuring the ratio of waist to hip circumference with metline instruments. Testing the relationship between variables was analyzed using the chi-square test. The result 52.2% of the sample had a higher percentage of body fat. 60.9% of the sample had less fiber intake. 58.7% of the sample had a higher waist to hip ratio. 52.2% of the sample had hypertension I. There was a relationship between the percent of body fat (pvalue0.001), fiberintake (p value 0.001), and ratio of waist to hip circumference (p value 0.001) with blood pressure. Penelitian ini bertujuan untuk mengetahui hubungan antara persentase lemak tubuh, asupan serat, dan rasio lingkar pinggang pinggul (RLPP) dengan tekanan darah lansia. Jenis penelitian ini menggunakan studi observasional analitik dengan pendekatan cross sectional, dilakukan terhadap 46 orang lanjut usia dengan purposive sampling. Pengukuran persentase lemak tubuh dengan instrumen BIA. Pengukuran asupan serat dengan instrumen food recall 2x24 jam, RLPP dengan instrumen metline, tekanan darah dengan sphygmomanometer. Uji hubungan yang digunakan adalah uji chi-square. Hasil penelitian menunjukkan bahwa 52,2% sampel memiliki persen lemak tubuh yang lebih. Rerata persen lemak tubuh laki-laki dan perempuan adalah 20,20% dan 25,29%. Sebanyak 60,9% sampel memiliki asupan serat yang kurang. Rerata asupan serat sebesar 22,59 gram per hari. 58,7% sampel memiliki rasio lingkar pinggang pinggul yang lebih. Rerata RLPP laki-laki dan perempuan adalah 0,92 dan 0.84. 52,2% sampel mengalami hipertensiI. Rerata tekanan darah sebesar 158/98 mmHg. Terdapat hubungan antara persentase lemak tubuh (p value = 0,000), asupan serat (p value = 0,000), dan rasio lingkar pinggang pinggul (p value =0,000) dengan tekanan darah.
Phytoremediation of domestic sewage using a floating wetland and assessing the pollutant removal effectiveness of four terrestrial plant species
Authors: D. Arivukkarasu , R. Sathyanathan 
Published: April 2023

Abstract
Several developing countries have limited infrastructure and finance to treat domestic and industrial wastewater. Discharging untreated sewage pollutes the surface and groundwater. Floating wetlands are an alternate method for treating polluted surface water bodies. This study's objective is to investigate the remediation of domestic wastewater using natural buoyant bamboo as a floating raft and terrestrial plants such as Ocimum tenuiflorum, Hibiscus, Chrysopogon zizanioides, and Canna in the floating wetland treatment (FWT) system. Floating rafts with a healthy terrestrial plant were planted and made to float in four plastic tanks with domestic wastewater. The water quality analysis was carried out periodically after 0, 3, 5, 10, 15, 20, and 25 days intervals. The experimental results of FWT using C. indica showed the highest removal efficiency of the pollutants such as TSS (96%), TP (98%), ammonia (95%), and DO (45%). In contrast, Ch. zizanioides showed its maximum removal efficiencies for turbidity (90%), TDS (48%), TN (85%), sodium (53%), potassium (74%), TP (92%), EC (27%), COD (93%), BOD (95%), and E. coli (47%). This study finding showed that the best terrestrial plants for removing various nutrients and other contaminants from municipal sewage were C. indica and Ch. zizanioides. However, further research is required to utilize these terrestrial plants with substrates under long-term study.
Coupling support vector machine and the irrigation water quality index to assess groundwater quality suitability for irrigation practices in the Tana sub-basin, Ethiopia
Authors: Berhanu Kibru Gedam , Hatiye Samuel Dagalo , Lohani Tarun Kumar 
Published: April 2023

AbstractLong-term and sustainable agricultural practices can be achieved through monitoring and evaluation of groundwater quality for irrigation operations. However, less attention was given to irrigation water quality in the Tana sub-basin, Ethiopia. The present study is aimed to assess the seasonal and spatial groundwater suitability for irrigation uses. The groundwater quality parameters measured in 40 samples in each dry and wet season were the pH, electrical conductivity (EC), Na+, Ca2+, Mg2+, K+, Fe2+, HCO3-, CO32-, Cl−, and NO3-. The groundwater suitability for irrigation was assessed using the irrigation water quality index (IWQI) and support vector machine (SVM). The results showed high and medium irrigation suitability classes in the dry and wet seasons. The proportion of groundwater samples in the medium irrigation suitability class in the dry and wet seasons, respectively, was 72.5 and 67.5%. The groundwater in the wet season is comparatively more suitable than that in the dry season, which is attributed to the leaching of accumulated salts during the wet season. To avoid a salinity threat, vigilance should be exercised when using groundwater during the dry seasons. The groundwater quality map developed here for irrigation may aid in locating better-quality groundwater sources for irrigation.