bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
Authors: Mega Ryosuke , Kim June-Sik , Tanaka Hiroyuki , Ishii Takayoshi , Abe Fumitaka , Okamoto Masanori 
Published: March 2023

Abstract
Bread wheat (Triticum aestivum) is one of the most important staple foods globally. Drought is a problem to decline wheat yield performance. Although much research has been performed to generate higher yield wheat cultivars, there have been few studies on improving end-product quality under drought stressed conditions, even though wheat is processed into flour to produce bread, noodles, pancakes, cakes, and cookies. Recently, wheat cultivation has been affected by severe drought caused by global climate change. In previous studies, seed shrinkage was observed in wheat exposed to continuous drought stress during seed development. In this study, we investigated how progressive drought stress affected seed development by metabolomic and transcriptomic analyses. Metabolite profiling revealed reduced accumulation of proline and sugar in the drought-sensitive line compared with the water-saving drought-tolerant transgenic line under drought conditions in spikelets with developing seeds. Meanwhile, the expressions of genes involved in translation, starch biosynthesis, and proline and arginine biosynthesis was downregulated in the drought-sensitive line. These findings suggest that seed shrinkage, i.e., endosperm deficiency, occurred because the biosynthesis of seed storage proteins, starch, amino acids, and sugars were inhibited since these ingredients did not accumulate in spikelets. Water-saving drought tolerant trait of wheat could contributes to support seed formation under drought.
Copula-based Reliability Analysis for Multicomponent System Stress-Strength Model under Progressively Censoring
Authors: Cai Jing , Yang Jianfeng , Wu Min , Yu Yarong 
Published: March 2023

Abstract
In reliability analysis, the stress-strength model is of importance and interest issue. Usually, the stress variable and the strength variable are assumed to be independent. However, such an assumption may be unrealistic in some applications. In this paper, we consider the statistical analysis of multicomponent system stress-strength model when the stress and strength are dependent variables, and the dependency is modeled by two different copula functions. We obtain the maximum likelihood estimates of the model parameters and the expression for the probability R=P(Y<X). Confidence intervals are constructed based on the asymptotic normality of maximum likelihood estimate. In addition, to test the dependence between the stress and strength variables, we discuss the likelihood ratio tests for hypotheses of interest. Finally, simulation studies and one real data example are provided to support the proposed model and methods, and to examine the performance of estimators and testing.
Core decompression combined with local DFO administration loaded on Polylactic Glycolic Acid scaffolds for the treatment of osteonecrosis of the femoral head: A pilot study
Authors: Gharanizadeh Kaveh , Sharifi Ali Mohammad , Tayyebi Hamed , Heidari Razieh , Amiri Shayan , Noorigaravand Sajad 
Published: March 2023

Abstract
Background

Deferoxamine (DFO) angiogenesis induction potential has been demonstrated in earlier studies, but not in the osteonecrosis of the femoral head (ONFH). In this study, we evaluated the outcome of ONFH treated with combined core decompression and local DFO administration loaded on Polylactic Glycolic Acid (PLGA).

Patients and Methods‎:

‎In a pilot experimental study‎,‎‏ six patients (‏‎10 ‎hips‎) ‎with early‎-‎stage non‎-‎traumatic ONFH were treated by ‏core decompression, and concurrent injection of local DFO ‎loaded on PLGA scaffold into the subchondral femoral head. Outcome measures were evaluated ‎before the surgery and 12 and 24 months after the surgery and included visual analog scale (VAS) for ‎pain, modified Merle d'Aubigné-Postel (MAP) score for hip function, and rate of osteonecrosis ‎assessed by the modified Kerboul angle. The incidence of the collapse was assessed by CT ‎scanning.‎

Results

The mean MPA score was 14.7 ± 1.16 before the surgery and 16.7 ± 1.41 one year after the surgery (P = 0.004). The mean VAS for pain was 4.7 ± 1.25 before the surgery and 1.8 ± 1.03 one year after the surgery (P = 0.005). The mean Kerboul angle was 219 ± 58.64 before the operation and 164.6 ± 41.82 one year after the operation (P < 0.001). Osteonecrosis progression or collapse was not seen in any of the patients at the final follow-up. No postoperative side effect attributed to the DFO was noticed, as well.

Conclusion

The mean MPA score was 14.7 ± 1.16 before the surgery and 16.7 ± 1.41 one year after the surgery (P = 0.004). The mean VAS for pain was 4.7 ± 1.25 before the surgery and 1.8 ± 1.03 one year after the surgery (P = 0.005). The mean Kerboul angle was 219 ± 58.64 before the operation and 164.6 ± 41.82 one year after the operation (P < 0.001). Osteonecrosis progression or collapse was not seen in any of the patients at the final follow-up. No postoperative side effects attributed to DFO were noticed, as well.
Trial registration: IR.IUMS.FMD.REC.1397.281
Kimchi exerts chemopreventive potential against colorectal carcinogenesis associated with red meat intake
Authors: Surya Reggie , Surya Ervan , Nugroho David , Romulo Andreas , Kamal Nurkhalida , Rahayu Winiati Pudji , Benchawattananon Rachadaporn , Oh Jin-Seok 
Published: March 2023

Abstract
Kimchi, the traditional fermented vegetables with seasonings from Korea, is globally renowned as healthy food with anticancer properties. Colorectal cancer is the world’s third leading cancer with an increasing incidence rate over the past years. High red meat intake is strongly associated with colorectal cancer and the World Health Organization has identified red meat as probably carcinogenic to humans (Group 2A). This study aimed to investigate the chemopreventive potential of cabbage kimchi against the promotion of colorectal cancer by red meat in vivo using 1,2-dimethyhydrazine (DMH)-injected rats. Kimchi supplementation at moderate (5%) and high (10%) amount significantly suppressed the formation of precancerous lesions in the colon of red meat-fed rats. Using fecal water, the aqueous extract of feces, we found out that kimchi supplementation limited iron-mediated oxidation and reduced lipid peroxidation in the feces of rats. Furthermore, kimchi supplementation reduced the toxicity of fecal water of red meat-fed rats towards human colonic epithelial cells in vitro by suppressing the formation of cellular reactive oxygen species (ROS). The chemopreventive effects of kimchi were associated with acidification of fecal matrix and increase in fecal lactic acid bacteria (LAB). Gene expression analysis in the colon of rats demonstrated that kimchi supplementation prevented colorectal carcinogenesis by up-regulating the expression of tumor suppressor genes and antioxidant enzymes, as well as by down-regulating the expression of proinflammatory proteins. Taken together, our findings suggested that consuming kimchi at moderate or high amount could protect against colorectal cancer promotion associated with high red meat intake.
A qualitative needs assessment of external communication by rare disease associations
Authors: Levine James A , Delval Florian , Kitchen A , Tena Garrigues 
Published: March 2023

Abstract
- Background. There is widespread acknowledgment amongst the rare disease community that effective communication strategies are lacking to explain diagnostic issues and therapeutic challenges to the public. The goal of this study was to objectively assess the communication needs of rare disease associations using validated techniques.
- Methods. Sixty-minute structured interviews and objective analysis of transcribed responses were conducted and analyzed for 20 rare disease organizations.
- Results. Associations uniformly agreed that effective communication was important to educate the public, advance advocacy and raise funds. However, there was no consensus as to how this should be achieved. Only seven of the 20 organizations had formal communication plans. Only few organizations had staff dedicated to external communication.
- Conclusions. Strategic improvement in rare disease communication may increase the awareness of rare diseases and advance science. Communication challenges included staffing, funding, technological resources, training and expertise.
Evaluation of human health and ecological risk of heavy metals in water, sediment and shellfishes in typical artisanal oil mining areas of Nigeria.
Authors: Chris Davies Ibienebo , Onyena Amarachi Paschaline , Sam Kabari 
Published: March 2023

Abstract
Heavy metal contamination in water and sediment is a serious concern, particularly in natural resource-dependent countries. In most coastal communities in these countries, drinking water quality, stable food, and livelihoods are primarily dependent on ecological systems and marine resources (e.g., sediments and fish), thus humans and other receptors are exposed to heavy metal risks through ingestion and dermal contact. This research evaluated the potential ecological risks of heavy metals including Cadmium (Cd), Chromium (Cr), Nickel (Ni) and Lead (Pb) in water, sediments, and shellfishes (C. amnicola, U. tangeri, T. fuscatus, P. monodon) along the Oproama creeks in Niger Delta, Nigeria. The concentrations of heavy metals were measured in three stations within the waterways using the Atomic Absorption Spectrophotometer (AAS) and their relative ecological and human health risk analysed. Health risk evaluation of individual heavy metals in shellfish tissue indicates an adverse health impact. The high mean carcinogenic and non-carcinogenic hazard index (HI) values of Cd (3.74), Pb (4.3), and Cr (8.4) recorded in the shellfish were significantly greater than 1, while the degree of contamination in the sediment (110.84) and water (60.15) were greater than 24 which is an indication of potential adverse ecological and human health impacts for different age groups and residents in the area. This establishes a significant possibility of heavy metal risks to public health, water quality and marine organisms. While in-depth health analysis is recommended, there is an urgent need to reduce oil spills, and an intentional provision of sustainable livelihoods to the local population.
Phylogenetic relationships of five date palm (Phonex dactylifera L) in Al Ahsa, Kingdom of Saudi Arabia using RAPD markers through QIAxcel system
Authors: Ahamed Reyas , Buwasis Hussai , Alsultan Musaed , Rezk Adel , solliman mohei el-din , Latif Abdul 
Published: March 2023

Abstract
Date palm (Phoenix dactylifera L.) is the sweetest edible fruit plant in the Kingdom of Saudi Arabia. The development of precise DNA fingerprints to identify cultivar's genetic diversity is most important for breeding programs. Genotypic variation among five different cultivars of date palm of the Al Ahsa governorate in Kingdom of Saudi Arabia was studied using random amplified polymorphic DNA (RAPD) markers by QIAxcel electrophoresis system. We test 20 RAPD primers among them 15 primers show banding pattern. We use clean younger leaves that had been gathered and grind with liquid nitrogen into a fine powder and used for extraction and ultimately the quality of the DNA. We examined the DNA extraction with the use of guide CTAB and automatic Qiagen EZ1 technique and tested using the UV gel electrophoresis the concentration of the DNA became examined with LABTRON Nano spectrophotometer. Four primers gave high levels of bands with numerous polymorphisms and multiple bands. A total of 165 bands were observed, including 89 polymorphisms and 4 monomorphisms. The Rz breed is very closely related to Kh, but the recorded Rz and Shb breeds retain a high degree of genetic divergence between Ht and Sh. The results of this study demonstrate that polymorphisms revealed using RAPD markers are genotype appropriate.
Estrogen inhibits the differentiation of fibroblasts induced by high stiffness matrix by enhancing DNMT1 expression
Authors: Zhao Zhihan , Huang Guotao , He Yong , Zuo Xiaohu , Han Wuyue , Hong Li 
Published: March 2023

Abstract
Introduction and Hypothesis:
Pelvic organ prolapse(POP) is a multifactorial connective tissue disorder caused by damage to the supporting structures of the pelvic floor. Evidence from several studies suggests that anterior vaginal wall stiffness is higher in patients with POP, but the mechanisms involved remain unknown.
Methods
Tissue from the anterior vaginal wall of patients with POP or other benign diseases was obtained. The modulus of elasticity of the anterior vaginal wall was measured using a microindenter. Cells were cultured in vitro on acrylamide gels of different stiffness and treated with DNMT1 inhibitor, microtubule polymerisation inhibitor and estrogen. Western blot or immunohistochemical staining was performed to detect DNA Methyltransferase 1, α-smooth muscle actin(α-SMA) expression, and connective tissue growth factor(CTGF) expression.
Conclusion
Estrogen can inhibit high stiffness matrix-induced fibroblast differentiation, by enhancing DNMT1 expression. This study may help to elucidate the complex crosstalk between fibroblasts and their surrounding matrix under healthy and pathological conditions and provide new insights into the options for material-related therapeutic applications.
An Atomically Thin and Photosensitive Vanadium Disulfide Memtransistor
Authors: Das Mayukh , Wali Akshay , Patel Amit , Rai Suyash , Puthirath Anand. , Ajayan Pulickel , Srivast Anchal , Das Saptarshi 
Published: March 2023

Abstract
Intrinsically ferromagnetic and semiconducting two-dimensional (2D) H-phase vanadium disulfide (VS2) holds tremendous promise for future electronics, optoelectronics, spintronics and valleytronics applications. However, its thermodynamic instability and the formation of intermediate stoichiometric polymorphs during its growth have stymied any progress towards synthesis of high quality 2D VS2 films. In this article, we circumvent these challenges and accomplish large area growth of monolayer VS2 films using atmospheric pressure chemical vapor deposition (APCVD) technique. By incorporating excess sulfur during the growth process which suppresses the formation of intermediate compounds, good quality large-area VS2 film can be synthesized. Furthermore, the electronic and optoelectronic properties of VS2 were explored by fabricating photosensitive memtransistor devices, which reveal an n-type carrier transport along with a high responsivity to red, green, and blue wavelengths of light. In addition the device exhibited multiple non-volatile conductance states through electrical programming. To the best of our knowledge, this is the first comprehensive report on memtransistors built from large area grown H-phase VS2 that integrate compute, sense, and storage functionalities in a single device.
Pattern of ROS generation and interconversion on wet stigmas in basal and divergent angiosperms
Authors: Breygina Maria , Luneva Oksana , Schekaleva Olga , Lazareva Nadezhda , Babushkina Ksenia , Kirilyuk Igor 
Published: March 2023

Abstract
The active participation of ROS in the reproductive physiology of flowering plants is well-documented in model species, mainly plants with dry stigmas. Here we studied the release of ROS into stigma exudate – a fluid involved in pollen acceptance on a wet stigma. The study involved plants from different systematic groups: Paeonia suffruticosa, Nicotiana tabacum, Lilium hybr., Aristolochia manshuriensis and Berberis vulgaris; in most cases, exudate was collected from intact flowers on the whole plant to minimize the effect of experimental intervention on the sensitive redox balance system. The dynamics of total oxidizing capacity of stigma exudate was assessed by EPR spectroscopy and different stigma staining. The level of hydrogen peroxide and the activity of superoxide dismutase, which catalyzes H2O2 formation, was also evaluated. In all the plants studied, total ROS level increased with stigma maturation, however, H2O2 concentration decreased in studied representatives of eudicots, except for peony. In lily it increased, while in birthwort it remained low, which was apparently due to the absence of SOD activity. In all divergent plants studied, the enzyme was active on stigmas with two isoenzymes. During the fertile period, activity was higher than at an earlier stage. Based on the obtained data, we speculate on primitive and progressive patterns of ROS generation and transformation on stigma and its possible evolution.
The Effect of Different Concentrations of Anti-PD-1 and Anti-PD-L1 Antibodies on the Activity of Immune System Cells in Patients With Non-small Cell Lung Cancer
Authors: Bożyk Aleksandra , Wojas-Krawczyk Kamila , Nicoś Marcin , Krawczyk Paweł 
Published: March 2023

Abstract

The last century was full of numerous scientific discoveries that allowed us to understand the operation and functioning of one of the most complex human systems, i.e. the immune system. One of the most important discoveries was the work of prof. James Alison and Prof. Tasuko Honjo on the development of anti-cancer therapy by inhibiting negative immune regulation (PD-1 and CTLA-4 molecules). Knowledge of the action of these molecules and their huge role in inhibiting the activity of the immune system, e.g. during the developing cancer, created the basis for the development of specific monoclonal antibodies, without which clinicians of many specialties cannot imagine modern cancer therapies. However, the side effects of the therapies used are still quite troublesome, as they can even lead to the death of the patient. In order to minimize the side effects, it would be necessary to reduce the dose, but still maintain the effective level of anticancer activity of the cells of the immune system. In the presented study, 24-hour cultures of PBMC isolated from peripheral blood and bronchoaspirate with various concentrations of nivolumab or atezolizumab were performed. The expression results of the individual activation markers on cultured cells were compared to the expression of these markers on cells not subjected to cell culture. The results of the research work may indicate that individualized dosage of anti-PD-1 and anti-PD-L1 antibodies may in the future contribute to the effective activation of the immune system cells while minimizing the side effects of the therapy.
Sociodemographic and environmental factors associated with diarrhoeal illness in children under 5 years in Uganda, 2021: A Cross-sectional study
Authors: Ssekandi Nathan , Tlotleng Nonhlanhla , Naicker Nisha 
Published: March 2023

Abstract
Background: Uganda is among the 10 countries in the sub-Saharan Africa region that have the highest prevalence of diarrhoeal disease. Evidence suggests that the severity of childhood diarrhoeal disease is escalated through various sociodemographic and environmental factors.
Objectives: To assess prevalence of diarrheal illness in children below the age of 5 years in Uganda in 2021 and associated factors.
Methods: A cross-sectional study was employed that analyzed secondary data from the 2021 Uganda Demography and Health Surveys. Statistically significant differences for children with and without diarrhea were compared. A logistic regression was used to determine sociodemographic and environmental factors associated with diarrheal illness in children.
Results: The prevalence of childhood diarrheal illness for children below the age of 5 years in Uganda was 20.9% (n = 2838/13,753). There was a statistically significant difference when comparing children diarrhoea for caregiver’s age, child’s age and sex and duration of breastfeeding (p<0.0001). Children with a caregiver aged between 15–24 years (aOR;1.42; 95% CI:1.24–1.62) and 25–34 years (aOR;1.19; 95% CI:1.04–1.37) were more likely to report diarrhoeal disease, compared to those with a caregiver aged 35–49 years. Households using springs, access to health facility and children who received a dose of vitamin A had a decreased risk of reporting children diarrhoeal.
Conclusion: Significant factors in the study like caregiver’s age, sex and duration of breastfeeding will create the opportunity for all interventions to shift their focus to these factors thus a better evidence-based approach to reducing of diarrhoeal disease will be achieved in the country
Extreme seasonal water-level changes and hydraulic modeling of deep, high-altitude, glacial-carved, Himalayan lakes
Authors: Camassa Roberto , Eidam Emily , Leve Lauren , McLaughlin Richard , Seim Harvey , Sharma Subodh 
Published: March 2023

Abstract
Himalayan lakes represent critical water resources, culturally important wa- terbodies, and potential hazards. Some of these experience dramatic water- level changes, responding to seasonal monsoon rains and post-monsoonal drain- ing. We present in situ observations of extreme (>12 meter) seasonal water- level changes in a 50-m deep lateral-moraine-dammed lake (lacking surface outflow), during a 16-month period, equivalent to a 5x106 m3 volume change annually. Data were used to construct a hydraulic model, which was used to estimate hydraulic conductivities of the moraine soils damming the lake. A higher moraine-dammed lake exhibits similar fluctuations. These lake levels lie above the nearby glacier surface. Several other lakes in the region exhibit minor seasonal water-level fluctuations, but each discharge through a surface outflow and lie below the glacier surface elevation. These results indicate that adjacent lakes may exhibit very different filling/draining dynamics and have different fates in the next few decades, based on presence/absence of surface outflows and elevation relative to retreating glaciers.
Impact of structurally diverse polysaccharides on colonic mucin O-glycosylation and gut microbiota
Authors: Zhao Tong , Zhang Yue , Nan Linhua , Zhu Qing , Wang Shukai , Xie Yutao , Dong Xinling , Cao Cui , Lin Xiaoliang , Lu Yu , Liu Yuxia , Huang Linjuan , Gong Guiping , Wang Zhongfu 
Published: March 2023

Abstract
Understanding how dietary polysaccharides affect mucin O-glycosylation and gut microbiota could provide nutrition-based treatments for individuals. In this study, the O-glycan profile and gut microbiome were investigated in C57BL/6J mice fed six structurally diverse dietary polysaccharides and a mixture of six fibers. Dietary polysaccharides increased total O-glycans mainly by increasing neutral glycans. Highly branched arabinogalactan significantly increased the level of terminally fucosylated core 1 O-glycans; whereas linear chains polysaccharides including pectin, konjac glucomannan, oligofructose (inulin), and mixture favored terminally di-fucosylated O-glycans. The last three also significantly lowered the level of sulfated O-glycans and sialylated mono-fucosylated O-glycans. Mixed polysaccharides had a synergistic effect, more significantly increasing fucosylated neutral glycans while decreasing acidic glycans, and stimulated microbial richness and diversity. Dietary polysaccharides containing arabinose and sulfate groups enhancedthe relative abundances of Akkermansia and Muribaculaceae, respectively. This systematic comparison reveals the relationship between dietary polysaccharide structure, mucin O-glycan composition, and intestinal microorganisms.