bluuebunny/crossref_metadata_2025_split huggingface dataset:
The loss of left atrial contractile function predicts a worse outcome in HFrEF patients
Authors: Mandoli Giulia Elena , Pastore Maria Concetta , Benfari Giovanni , Setti Martina , Maritan Luca , Diviggiano Enrico Emilio , D’Ascenzi Flavio , Focardi Marta , Cavigli Luna , Valente Serafina , Cameli Matteo 
Published: January 2023

BackgroundIn chronic heart failure, high intracardiac pressures induce a progressive remodeling of small pulmonary arteries up to pulmonary hypertension. At the end of left atrial conduit function, pulmonary and left heart end-systolic pressures equalization might affect left atrial systole. In this single-center prospective study, we aimed to investigate whether peak atrial contraction strain (PACS), measured by speckle tracking echocardiography, was independently associated with prognosis in heart failure with reduced ejection fraction (HFrEF).Materials and methodsOutpatients with HFrEF and sinus rhythm referred to our echo-labs were enrolled. After clinical and echocardiographic evaluation, off-line speckle tracking echocardiography analysis was performed. Primary and secondary endpoint were cardiovascular death and heart failure hospitalization, respectively. Spline knotted survival model identified the optimal prognostic cut-off for PACS.ResultsThe 152 patients were stratified based on PACS &lt;8% (n = 76) or PACS ≥8% (n = 76). Patients with PACS &lt;8% had lower left ventricle and left atrial reservoir strain and higher New York Heart Association (NYHA) class and left atrial volume index (LAVI). Over a mean follow-up of 3.4 ± 2 years, 117 events (51 cardiovascular death, 66 heart failure hospitalizations) were collected. By univariate and multivariate Cox analysis, PACS emerged as a strong and independent predictor of cardiovascular death and heart failure hospitalization, after adjusting for age, sex, left ventricle strain, and E/e’, LAVI (HR 0.6 per 5 unit-decrease in PACS). Kaplan–Meier curves showed a sustained divergence in event-free survival rates for the two groups.ConclusionThe reduction of PACS significantly and independently affects cardiovascular outcome in HFrEF. Therefore, its assessment, although limited to patients with sinus rhythm, could offer additive prognostic information for HFrEF patients.
The effects of experimental, meteorological, and physiological factors on short-term repeated pulse wave velocity measurements, and measurement difficulties: A randomized crossover study with two devices
Authors: Podrug Mario , Šunjić Borna , Bekavac Anamarija , Koren Pjero , Đogaš Varja , Mudnić Ivana , Boban Mladen , Jerončić Ana 
Published: January 2023

BackgroundLarge longitudinal studies with repeated pulse wave velocity (PWV) measurements, a direct measure of arterial stiffness, are required to realize the full potential of arterial stiffness in clinical practice. To facilitate such studies it is important to increase the power of a study by reducing within-subject variability of PWV, and to ease the use of a PWV device in clinical settings by minimizing PWV measurement difficulties.MethodsWe systematically investigated experimental setting and meteorological conditions, as well as physiological factors and participant characteristics, to determine whether and to what extent they affected: between- and within-subjects variability of PWV recordings, and measurement difficulties of a particular device. We conducted a 2-week longitudinal block-randomized cross-over study with two blinded observers and two commonly used devices: applanation tonometry SphygmoCor CvMS and oscillometric Arteriograph to assess carotid-femoral (cfPWV) or aortic (PWVao) PWV, respectively. Our sample had uniform and wide-spread distribution of age, blood pressures, hypertensive status and BMI. Each participant (N = 35) was recorded 12 times over 3 visiting days, 7 days apart. On each day, recordings were made twice in the morning (7–10 a.m.) and afternoon (16–18 p.m.). Data were analyzed using multilevel mixed-effects models, separately for each device.ResultsIn addition to age and mean arterial pressure (MAP) that strongly affected both cfPWV and PWVao, other significant factors appeared to indicate a measurement approach. cfPWV as a more direct measure of arterial stiffness was additionally affected by hypertension status, outdoor temperature, interaction of MAP with outdoor temperature and the order of visit, with MAP within-subject variability contributing on average 0.27 m/s to difference in repeated measurements at 5°C and 0.004 m/s at 25°C. PWVao measurements derived at a single brachial site were more dependent on age than cfPWV and also depended on personal characteristics such as height and sex, and heart rate; with within-subject MAP variability adding on average 0.23 m/s to the difference in repeated measures. We also found that female sex significantly increased, and recording in afternoon vs. morning significantly decreased measurement difficulties of both devices.ConclusionWe identified factors affecting PWV recordings and measurement-difficulties and propose how to improve PWV measuring protocols.
Research and application of pressure relief and permeability improvement in high gas outburst mines by directional drilling and hydraulic jet
Authors: Li Dingqi , Chen Yubo , Zhang Jiabao , Wang Mingzhong 
Published: January 2023

Efficient gas extraction technology is an important topic for low permeability and high gas outburst coal seam. Based on the engineering background of Hudi Coal Mine with the soft and hard coal seams and the existing hydraulic permeability enhancement technology, a new construction process to improve coal seam permeability was proposed to effectively reduce coal seam gas content and the risk of coal and gas outburst. In this measure, the roadway in floor was replaced with a directional main borehole, directional branch boreholes were used to replace crossing holes, and soft coal was mined along soft sub layers via the directional drilling machine and directional hydraulic jet. Main boreholes are drilled parallel to the seam in the coal seam floor, and branch boreholes are drilled through the floor and coal seam. The numerical simulation was used to study the permeability improvement effect of different mining diameters by the proposed measure. The result showed that, as the mining diameter increased from 2 m to 4 m, the average influence diameter of coal seam porosity increased from 15.44 m to 19.65 m, and the average influence diameter of the permeability increased from 15.75 m to 20.07 m, which is three times the influence range of the ordinary borehole. The application of the proposed measure and its supporting equipment was carried out under the special coal seam and gas conditions of Hudi Coal Mine. Results show that the soft coal was mined efficiently along the soft sub layer using the main borehole, branch boreholes, and directional hydraulic jet. Compared the traditional hydraulic flushing in the borehole with the ordinary drilling machine, the average speed of mining soft sub layers increased from 0.5 t/h to 3.6 t/h, the equivalent mining diameter of soft sub layers increased from 1.2 m to 7.6 m, and the average flow of gas extraction increased from 0.41 m3/d to 6.25 m3/d. The conclusions obtained in this study can provide a reference for coal mine gas extraction with similar coal seam conditions.
Research on safety early warning of abnormal A-annular pressure of “three-high” gas wells based on data mining
Authors: Yan Hongqiao , Qi Junliang , Wu Shuncheng , Wang Shunyi , Zhong B. T. 
Published: January 2023

A major safety accident will be triggered when the A-annular pressure value of high pressure, high productivity and high sulfur gas well exceeded the maximum allowable value. The A-annular pressure value of high pressure high productivity and high sulfur gas well once exceeded the allowable value will trigger a major safety accident. Therefore, this paper proposed a data mining based the gas well early warning strategy by analyzing the annular pressure mechanism and the change in the pattern of instantaneous gas volume, well temperature, and annular pressure in various conditions. To summarize, the law of gas well abnormal A-annular pressure is aimed at constructing a gas well safety warning rule for gas well stable production stage and shutdown period where the initial parameters setting in the early warning rule and adjustment optimization mechanism is also determined. Lastly, with the use of historical abnormal samples, the gas well early warning strategy bought up in this paper was verified. The example shown that compared with the traditional DCS system warning strategy, the gas well safety early warning strategy can identify the abnormal A-annular pressure phenomenon 82 h in advance thus achieving production safety management.
Hydrothermal fluid characteristics of the huayuan Pb-Zn orefield: Constraints from apatite major and trace element compositions
Authors: Tan Rui-Chang , Shao Yong-Jun , Wei Han-Tao , Zhang Jun-Ke , Yu Meng-Da , Xiong Yi-Qu 
Published: January 2023

The Huayuan orefield in the SW Yangtze Block (SW China) is a world-class Pb-Zn orefield, with over 20 million tonnes (Mt) metal reserve. However, the Pb-Zn ore fluid source and evolution in Huayuan remain controversial. This study determined the major and trace element compositions of the newly-identified apatite from the Pb-Zn ores, using electron microprobe analysis (EMPA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The apatite samples are of hydrothermal origin, and have high CaO (52.57–57.15 wt%), P2O5 (39.26–42.88 wt%) and F (1.82–3.90 wt%) but low Cl (&lt; 0.42 wt%) contents. The samples have total rare Earth element content (ΣREE) of 74.07–1,255.34 ppm, and they all show negative Eu and weakly positive Ce anomalies. The result suggests that the apatite was formed in an environment with decreasing oxygen fugacity, and in relatively F-rich, Cl-poor, and REE-poor ore-forming fluid. We geochemically compared the apatite from Huayuan with those from different geneses by Fisher discriminant. The result suggests that the Huayuan apatite is distinct from typical magmatic and purely hydrothermal apatite, and that the ore-forming fluids may have had multiple sources. The mixing of fluids with different origins may have triggered significant metal ore deposition.
Application of horizontal well discontinuous chemical flooding technology in Bohai oilfield
Authors: Jian Zhang , Dan Liang , Liqi Wang , Engao Tang , Xianjie Li , Wensen Zhao 
Published: January 2023

Discontinuous chemical flooding technology of horizontal wells is a long-term development mechanism of horizontal wells established by combining and applying various oil displacement systems in different stages. Discontinuous chemical flooding not only ensures the injection capacity of chemical flooding stage, but also saves the operation cost of chemical flooding, and maximizes the economic benefits of crude oil production while improving the oil recovery. Based on the reservoir physical properties and fluid physical parameters of Bohai B oilfield, the development scheme of discontinuous chemical flooding in horizontal wells is studied on the basis of laboratory experimental evaluation. According to the optimization results of injection parameters, four types of slugs are set, i.e., front slug, main slug, auxiliary slug and secondary slug. A variety of agent systems such as gel, pre crosslinked particles, polymer and acid solution are used. The slug adjustment timing is determined by the monitoring data and the analysis results of production dynamic characteristics. Under the conditions of 0.07 PV/a injection rate and 0.49 PV slug dosage, the recovery efficiency of discontinuous chemical flooding can be improved by 10.4% compared with water flooding. After the implementation of the field, the daily oil production of typical affected wells has shown a significant upward trend, with the peak increase of 135%–151% and the peak decrease of water cut of 15%. After 2 years and 6 months of implementation, chemical flooding has achieved a cumulative increase of 66,000 m3compared with basic water flooding, with the oil increase accounting for 41% of the output of the whole stage. The effect of water reduction and oil increase is remarkable. The research results show that discontinuous chemical flooding can be popularized and applied in the oil fields developed by horizontal wells, so as to control the rapid rising trend of water cut in the middle and high water cut stage and further improve the oil recovery.
Case report: Daratumumab for treatment of refractory late or chronic active antibody-mediated rejection in renal allograft recipients with high levels of de novo donor-specific antibodies
Authors: Zhu Lan , Guo Zhiliang , Zhao Daqiang , Sa Rula , Zhao Guangyuan , Guo Hui , Chen Gang 
Published: January 2023

BackgroundLate or chronic active antibody-mediated rejection (AMR) associated with de novo donor-specific antibodies (dnDSA) after renal transplantation is a great clinical challenge because it is often resistant to conventional therapies. Daratumumab, an anti-CD38 monoclonal antibody that can deplete plasma cells, may be effective for the treatment of late or chronic active AMR.MethodsWe designed a novel regimen that included early intensive therapy with daratumumab plus plasmapheresis (PP)/intravenous immunoglobulins (IVIG) and later maintenance therapy with daratumumab alone, and used this regimen to treat late or chronic active AMR in two kidney transplant recipients with extremely high levels of anti-DQ7 dnDSA.ResultsBoth patients had a limited clinical response to the early treatment with rituximab and PP/IVIG (with or without splenic irradiation); however, they had a remarkable decrease in anti-DQ7 DSA (MFI value from ~20,000 to ~5,000) after 2-3 months of intensive therapy with daratumumab plus PP/IVIG. Over 20 months of follow-up, patient 1 maintained a low DSA (as low as 1,572) and normal renal function on daratumumab maintenance therapy. Patient 2 retained a low DSA and improved renal function and pathological lesions within one year after treatment but then deteriorated because of acute T cell-mediated rejection.ConclusionsOur daratumumab-based regimen has shown promising results in the treatment of refractory late active or chronic active AMR in renal transplant recipients with high-level dnDSA. This may provide a reference for better use of daratumumab in the treatment of late or chronic active AMR.
Weight cycling induces innate immune memory in adipose tissue macrophages
Authors: Caslin Heather L. , Cottam Matthew A. , Piñon Jacqueline M. , Boney Likem Y. , Hasty Alyssa H. 
Published: January 2023

IntroductionWeight loss improves obesity-associated diabetes risk. However, most individuals regain weight, which worsens the risk of developing diabetes and cardiovascular disease. We previously reported that male mice retain obesity-associated immunological changes even after weight loss, suggesting that immune cells may remember the state of obesity. Therefore, we hypothesized that cycles of weight gain and loss, otherwise known as weight cycling, can induce innate memory in adipose macrophages.MethodsBone marrow derived macrophages were primed with palmitic acid or adipose tissue conditioned media in a culture model of innate immune memory. Mice also put on low fat or high fat diets over 14-27 weeks to induce weight gain, weight loss, and weight cycling. ResultsPriming cells with palmitic acid or adipose tissue conditioned media from obese mice increased maximal glycolysis and oxidative phosphorylation and increased LPS-induced TNFα and IL-6 production. Palmitic acid effects were dependent on TLR4 and impaired by methyltransferase inhibition and AMPK activation. While weight loss improved glucose tolerance in mice, adipose macrophages were primed for greater activation to subsequent stimulation by LPS ex vivo as measured by cytokine production. In the model of weight cycling, adipose macrophages had elevated metabolism and secreted higher levels of basal TNFα, suggesting that weight loss can also prime macrophages for heighted activation to weight regain.DiscussionTogether, these data suggest that weight loss following obesity can prime adipose macrophages for enhanced inflammation upon weight regain. This innate immune memory response may contribute to worsened glucose tolerance following weight cycling.
Notoginsenoside Fc ameliorates renal tubular injury and mitochondrial damage in acetaminophen-induced acute kidney injury partly by regulating SIRT3/SOD2 pathway
Authors: Wei Miaomiao , Gao Yuancheng , Cheng Dongsheng , Zhang Haiying , Zhang Wei , Shen Yilan , Huang Qunwei , An Xiaoning , Wang Bing , Yu Zhonghai , Wang Niansong , Chen Hongbo , Xu Youhua , Gui Dingkun 
Published: January 2023

IntroductionMitochondria dysfunction is one of the primary causes of tubular injury in acute kidney injury (AKI). Notoginsenoside Fc (Fc), a new saponin isolated from Panax notoginseng, exhibited numerous pharmacological actions. However, the beneficial effects of Fc on renal tubular impairment and mitochondrial dysfunction in AKI have not been fully studied.MethodsIn this study, we established acetaminophen (APAP)-induced AKI model in mice to examine the therapeutic impacts of Fc on AKI.ResultsOur results showed that Fc could decrease the levels of the serum creatinine (Scr), blood urea nitrogen (BUN) and Cystatin C in mice with AKI. Fc also ameliorated renal histopathology, renal tubular cells apoptosis and restored expression of apoptosis-related proteins such as Bax, Bcl-2 and caspase3 (C-caspase3). Additionally, Fc increased the protein expression of SIRT3 and SOD2 in kidneys from mice with AKI. In vitro studies further showed Fc reduced the apoptosis of HK-2 cells exposure to APAP, attenuated the loss of mitochondrial membrane potential and decreased the formation of mitochondrial superoxide. Fc also partly restored the protein expression of Bax, Bcl-2, C-Caspase3, SIRT3, and SOD2 in HK-2 cells exposure to APAP.ConclusionIn summary, Fc might reduce renal tubular injury and mitochondrial dysfunction in AKI partly through the regulation of SIRT3/SOD2 pathway.
Influence of structural features and feruloylation on fermentability and ability to modulate gut microbiota of arabinoxylan in in vitro fermentation
Authors: Li Zhongxia , Zhang Huibin , He Li , Hou Yaqin , Che Yingjuan , Liu Tian , Xiong Shaobai , Zhang Xuguang , Luo Shunjing , Liu Chengmei , Chen Tingting 
Published: January 2023

IntroductionArabinoxylan (AX) is a versatile polysaccharide that shows various effects in modulating gut microbiota and health. The influence of arabinoxylan carbohydrate structural feature and feruloylation on fermentability and the effect of modulation of gut microbiota of AX was not clear.MethodsArabinoxylans from rice bran and corn bran (RAX and CAX), and their deferulyolated counterpart dRAX and dCAX were fermented using an in vitro fermentation model. Structural information was determined based on monosaccharide composition. Gas production of fermentation products, SCFAs production, pH change, and microbiota change were measured.ResultsRAX and dRAX posessed lower A/X ratio compared with CAX and dCAX. The gas and total SCFAs production were lower in RAX and dRAX, and the butyrate production were higher in RAX and dRAX compared with CAX and dCAX. Butyrate production was lower at dRAX compared to RAX. On the other hand, butyrate production was higher in dCAX than in CAX. The microbiota shift were different for the four fibers.DiscussionThe AXs from rice have a higher A/X ratio than the AXs from maize, suggesting more branching and a more complex side chain. The structural difference was crucial for the difference in fermentation pattern. Different Bacteroides species are responsible for the utilization of rice AXs and corn AXs. Although feruloylation had a minor effect on the overall fermentation pattern, it significantly affected butyrate production and alpha diversity. dRAX promoted less butyrate than RAX, which is associated with a significantly lower amount of Faecalibacterium prausnitzi. dCAX promoted more butyrate than CAX, which may be associated with a lower amount of Bacteroides ovatus and a higher amount of Blautia in dCAX compared to CAX. The effects of feruloylation on the fermentation pattern and the resulted microbiota shift of AX varied depending on the carbohydrate structure.
Plasma enzymatic activity, proteomics and peptidomics in COVID-19-induced sepsis: A novel approach for the analysis of hemostasis
Authors: Dos Santos Fernando , Li Joyce B. , Juocys Nathalia , Mazor Rafi , Beretta Laura , Coufal Nicole G. , Lam Michael T. Y. , Odish Mazen F. , Irigoyen Maria Claudia , O’Donoghue Anthony J. , Aletti Federico , Kistler Erik B. 
Published: January 2023

Introduction: Infection by SARS-CoV-2 and subsequent COVID-19 can cause viral sepsis. We investigated plasma protease activity patterns in COVID-19-induced sepsis with bacterial superinfection, as well as plasma proteomics and peptidomics in order to assess the possible implications of enhanced proteolysis on major protein systems (e.g., coagulation).Methods: Patients (=4) admitted to the intensive care units (ICUs) at the University of California, San Diego (UCSD) Medical Center with confirmed positive test for COVID-19 by real-time reverse transcription polymerase chain reaction (RT-PCR) were enrolled in a study approved by the UCSD Institutional Review Board (IRB# 190699, Protocol #20-0006). Informed consent was obtained for the collection of blood samples and de-identified use of the data. Blood samples were collected at multiple time points and analyzed to quantify a) the circulating proteome and peptidome by mass spectrometry; b) the aminopeptidase activity in plasma; and c) the endopeptidase activity in plasma using fluorogenic substrates that are cleaved by trypsin-like endopeptidases, specific clotting factors and plasmin. The one patient who died was diagnosed with bacterial superinfection on day 7 after beginning of the study.Results: Spikes in protease activity (factor VII, trypsin-like activity), and corresponding increases in the intensity of peptides derived by hydrolysis of plasma proteins, especially of fibrinogen degradation products and downregulation of endogenous protease inhibitors were detected on day 7 for the patient who died. The activity of the analyzed proteases was stable in survivors.Discussion: The combination of multiomics and enzymatic activity quantification enabled to i) hypothesize that elevated proteolysis occurs in COVID-19-induced septic shock with bacterial superinfection, and ii) provide additional insight into malfunctioning protease-mediated systems, such as hemostasis.