bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic Resistance of Diverse MLST Campylobacter from Pediatric Patients with Acute Diarrhea
Authors: Hou Shuiping , Mei Peng , Shi Runfeng , Chen Kuncai , pan Xinhong , Chen shouyi , Li Juntao 
Published: July 2023

Abstract
Background
Campylobacter spp., as a zoonotic pathogen, has been one of the most common pathogens that causes human gastroenteritis in developing countries. The objective of this study was to determine the antibiotic resistance of Campylobacter in children.
Methods
Stool samples were collected from pediatric patients under 14 years of age who visited hospital for acute diarrhea between February 2018 and January 2019. Campylobacter was isolated from samples using selective enrichment and culture and confirmed by PCR, genotypes were characterized by multilocus sequencing, and antibiotic resistance was determined by susceptibility and resistance genes to antimicrobials of medical importance.
Results
The overall prevalence of Campylobacter in children with diarrhea was 4.4% (53/1197). Higher prevalence was observed in children under five-year-old and in spring and autumn. Approximately 80% (42/53) of confirmed isolates were C. jejuni and ~ 20% (11/53) isolates were C. coli. Among the 51 isolates successfully typed, 43 sequence types belonging to 13 clonal complexes were identified, of which 44.2% (19/43) sequence types were novel. The majority of the Campylobacter isolates (74%) exhibited multidrug resistance and the most common antimicrobials resistant to were ciprofloxacin, tetracycline and nalidixic acid. All isolates (53/53) were positive of the tet(O) gene that confers resistance to tetracycline and the gyrA gene that confers resistance to quinolones/fluoroquinolones, 81% (43/53) isolates were positive of the ermB gene that confers resistance to macrolides, and 19% (10/53) isolates were positive of the aadE-sat4-aphA gene that confers resistance to aminoglycosides, respectively.
Conclusions
Despite the low prevalence of Campylobacter in children in the subtropical Guangzhou metropolitan, the high multidrug resistance of diverse MLST of Campylobacter indicated the importance of preventive strategies including routine surveillance in order to minimize the infections of antibiotic resistant Campylobacter in children.
Physical Activity and Health-Related Quality of Life in Older Adults: Depression as a Mediator
Authors: Li Xiuxiu , Wang Pengfei , Jiang Yihua , Yang Yinghua , Wang Feng , Yan Fei , Peng Wenjia , Wang Ying 
Published: July 2023

Abstract
Background
Physical activity is associated with health-related quality of life (HRQoL) among older adults, and both are associated with mood, such as depression. However, the indirect effects of physical activity on HRQoL in older adults have not been clearly established. This study explains how physical activity is associated with HRQoL while considering the effects of depression in older adults.
Methods
A cross-sectional study was conducted with 7,518 community-dwelling older adults aged 60 years and older. Physical activity, depression, and HRQOL were measured using the Physical Activity Scale for the Elderly, the 30-item Geriatric Depression Scale, and the 36-Item Short-Form Health Survey (SF-36), respectively. Physical activity included three types of activities: leisure, household, and work. Information on age, gender, education, monthly income, physical functioning, smoking, and alcohol use were also collected.
Results
The multivariate analyses showed that after adjusting for sociodemographic variables, SF-36 Physical Component Summary scores were negatively associated with depression (B = -2.046, 95% CI [2.584, -1.509]) and positively with physical activity (p < 0.001). Similarly, SF-36 Mental Component Summary scores were negatively associated with depression (B = -11.657, 95% CI [-12.190, -11.124]). In the mediation analysis, different types and levels of physical activity positively correlated with HRQoL (p < 0.001), and depression negatively correlated with physical activity and HRQoL (p < 0.001).
Conclusions
The findings showed that lighter amounts of physical activity and worsening HRQoL in Chinese older adults were independently associated. In addition, the relationship between physical activity and HRQoL was partially mediated by depression. It is recommended that geriatric health care administrators and health care planners prioritize improving physical activity levels in older adults to promote the reduction of depressive symptoms in older adults to improve the beneficial effects on HRQoL.
The ENCODE Uniform Analysis Pipelines
Authors: Hitz Benjamin , Lee Jin-Wook , Jolanki Otto , Kagda Meenakshi , Graham Keenan , Sud Paul , Gabdank Idan , Strattan J. Seth , Sloan Cricket , Dreszer Timothy , Rowe Laurence , Podduturi Nikhil , Malladi Venkat , Chan Esther , Davidson Jean , Ho Marcus , Miyasato Stuart , Simison Matt , Tanaka Forrest , Luo Yunhai , Wahling Ian , Lin Khine Zin , Jou Jennifer , Hong Eurie , Lee Brian , Sandstrom Richard , Rynes Eric , Nelson Jemma , Nishida Andrew , Ingersoll Alyssa , Buckley Michael , Frerker Mark , Kim Daniel , Boley Nathan , Trout Diane , Dobin Alexander , Rahmanian Sorena , Wyman Dana , Balderrama-Gutierrez Gabriela , Reese Fairlie , Durand Neva , Dudchenko Olga , Weisz David , Rao Suhas , Blackburn Alyssa , Gkountaroulis Dimos , Sadr Mahdi , Olshansky Moshe , Eliaz Yossi , Nguyen Dat , Bochkov Ivan , Shamim Muhammad , Mahajan Ragini , Aiden Erez , Gingeras Thomas , Heath Simon , Hirst Martin , Kent W. James , Kundaje Anshul , Mortazavi Ali , Wold Barbara , Cherry J. 
Published: July 2023

Abstract
The Encyclopedia of DNA elements (ENCODE) project is a collaborative effort to create a comprehensive catalog of functional elements in the human genome. The current database comprises more than 19000 functional genomics experiments across more than 1000 cell lines and tissues using a wide array of experimental techniques to study the chromatin structure, regulatory and transcriptional landscape of the Homo sapiens and Mus musculus genomes. All experimental data, metadata, and associated computational analyses created by the ENCODE consortium are submitted to the Data Coordination Center (DCC) for validation, tracking, storage, and distribution to community resources and the scientific community. The ENCODE project has engineered and distributed uniform processing pipelines in order to promote data provenance and reproducibility as well as allow interoperability between genomic resources and other consortia. All data files, reference genome versions, software versions, and parameters used by the pipelines are captured and available via the ENCODE Portal. The pipeline code, developed using Docker and Workflow Description Language (WDL; https://openwdl.org/) is publicly available in GitHub, with images available on Dockerhub (https://hub.docker.com), enabling access to a diverse range of biomedical researchers. ENCODE pipelines maintained and used by the DCC can be installed to run on personal computers, local HPC clusters, or in cloud computing environments via Cromwell. Access to the pipelines and data via the cloud allows small labs the ability to use the data or software without access to institutional compute clusters. Standardization of the computational methodologies for analysis and quality control leads to comparable results from different ENCODE collections - a prerequisite for successful integrative analyses.
Prevalence and Associated Risk Factors of Resistant Hypertension among Chinese Hypertensive Patients in Primary Care Setting
Authors: Chan Kilpatrick Kiupak , Chiang Lapkin , Choi Clarence Chuenming , Li Yimchu , Chen Catherine Xiarui 
Published: July 2023

Abstract

Introduction:
Hypertension (HT) is a major public health problem globally, and it is the commonest chronic disease with a prevalence of 27% among people aged 15 years or above in Hong Kong. There is emerging literature confirmed that patients with resistant hypertension (RHT) give its increased risk for adverse clinical outcomes and higher rate of documented target organ damage. This study aims to identifythe prevalence of RHT among Chinese hypertensive patients managed in public primary care setting of Hong Kong and exploring its associated risk factors.
Methodology: This is a cross-sectional descriptive study. Chinese hypertensive patients aged 30 or above with regular follow-up between 1st July 2019 and 30th June 2020 in 10 public primary care clinics under the Hospital Authority of Hong Kong were included. Demographic data, clinical parameters and drug profile of patients were retrieved from its computerized record system. The prevalence of RHT was identified and the associated risk factors of RHT were explored by multivariate logistic regression analysis.
Results: Among the 538 sampled Chinese hypertensive patients, the mean age was 67.4 ±11.5 years old, and 51.9% were female. The mean duration of hypertension was 10.1 ± 6.4 years, with a mean systolic and diastolic blood pressure of 128 ± 12.3 and 74 ± 10.8 mmHg respectively. 40 out of 538 patients were found to have RHT, giving an overall prevalence of 7.43%. Four factors were found to be associated with increased risk of RHT, in ascending order of odds ratio: duration of hypertension (OR 1.08), male gender (OR 2.72), comorbid with type 2 diabetes mellitus (T2DM, OR 2.99), and congestive heart failure (CHF, OR 5.39).
Conclusion: The prevalence of RHT among Chinese hypertensive patients in primary care setting of Hong Kong is 7.43%. RHT is more common in male patients, patients with longer duration of hypertension, concomitant T2DM and CHF. Clinicians should be vigilant when managing these groups of patients and provide aggressive treatment and close monitoring.
Whitlockite nanoparticle-coated hydroxyapatite granules for bone regeneration in defect of rat calvaria
Authors: Lee Won-Bum , Wang Caifeng , Jang Yoon-Seo , Jeong Ki-Jae , Kang Hyojin , Seol Ayun , Park Eun Seo , Lee Ho-Jin , Hwang Dae Youn , Lee Jaebeom , Hwang Dae-Seok 
Published: July 2023

Abstract
Background
Whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) as the second most abundant ceramic of human bone after hydroxyapatite (HA; Ca10(PO4)6(OH)2), possessing high osteogenic activity, has been known that it can stimulate osteogenic differentiation as well as suppress osteoclastic activity, particularly, in the preparatory stage of bone regeneration.
Methods
In this work, we construct a unique ceramic structure by layering WH on the surface of HA granules (HAGs)s via dip-coating and sintering method.
Results
The cell proliferation of the WH-coated HAGs (WHHAGs) group in the cell counting kit (CCK-8) was about 1.15-fold higher than that of the NT group at 72 h after incubation. The western blot and qPCR results demonstrated that WHHAGs treatment readily stimulated the transcription of osteogenic genes by regulating the downstream signaling pathway of BMP and WNT receptors.This study presents a histological and radiological evaluation of the bone healing potential of the WHHAGs in a 7 mm diameter calvarial bone defect in rats, compared with/without the BMP and non-treated (NT) groups at the 8 weeks after surgery. The bone volume fraction (bone volume/tissue volume) of the WHHAGs group was about 7.32- and 3.56- fold higher than that of the NT group with/without the BMP, respectively. Besides, histological evaluation confirmed that the WHHAGs with BMP promoted bone regeneration.
Conclusion
These results suggest that the WHHAGs present the remarkable potential for application in dental and orthopedic bone regeneration.
Effect Of Molnupiravir On Ichemia/Reperfusion Damage In Rat Liver, An Experimental Study
Authors: TAS Hakan Gokalp , TAS Necip Gokhan , KUYRUKLUYILDIZ Ufuk , ZIRH Selim , KURT Nezahat 
Published: July 2023

Abstract
Background
Ischemia occurs when blood supply to tissues is limited, resulting in cellular dysfunction and necrosis. Reperfusion, or the process of restoring blood flow, can result in an overabundance of reactive oxygen species (ROS) and toxic byproducts. The I/R (Ischemia / Reperfusion) technique used in liver resection and transplantation has been linked to liver damage. Molnupiravir, a non-hepatotoxic oral medicine, is converted into N-hydroxycytidine (NHC). The purpose of this study is to see how molnupiravir affects I/R-induced damage of liver in rats.
Methods
We divided the rats into three groups: Sham operation (SG) (n = 6), Liver I/R (LIR) (n = 6), and Molnupiravir + Liver I/R (MIR) (n = 6) groups. The MIR group (n = 6) received 40 mg/kg of molnupiravir. All animals were subjected to laparotomy, hepatic ischemia (1 hour), and reperfusion (6 hours). At the end of the trial, liver tissue samples were tested for IL-1β, tGSH, NF-κB, MDA, and TNF-α levels, as well as histopathological examination. The levels of ALT and AST in the blood were determined. The MIR group's results were in comparison to the SG and LIR groups.
Results
Biochemical examination revealed that NF-ƘB, MDA, TNF-α, IL-1β, ALT, and AST measurements were higher in the LIR and MIR groups than in the SG group. The SG group had the highest tGSH values. Histopathological examinations revealed that the MIR group had the most damage.
Conclusion
While molnupiravir, which is included in COVID-19 treatment regimen since it has no projected liver toxicity, does not affect healthy liver tissue, it does exacerbate ischemia/reperfusion injury in stressed liver tissue. Molnupiravir should be used with caution because it has the potential to aggravate liver damage during procedures such as liver transplantation or resection.
Preliminary study of HPV integration status on the occurrence and development of Vaginal intraepithelial neoplasia
Authors: Yuan Shuning , Qian Cheng , Zhang Hailong , Xing Yan 
Published: July 2023

Abstract

Vaginal intraepithelial neoplasm (VaIN), a precancerous lesion of vaginal cancer, increased distinctly in the past decade, while its occurrence after performing hysterectomy came to attention nowadays. As hr-HPV DNA integrates into host chromosome, transient HPV infection could transform into persistent infection to trigger high-grade intraepithelial neoplasm or HPV-related cancer. High-throughput Viral Integration Detection (HIVID) is a novel genetic sequencing method detecting HPV integration breakpoints for more precise sequence. There is no systematic study on the mechanism and risk assessment prediction system of VaIN in the vaginal stump after total hysterectomy for CIN. In this study, 24 cases suffered from VaIN were involved. HPV integration was detected in 11 cases, furthermore, we discovered HPV 16 and 73, chromosome 1 and 2 possessed most HPV integration sites while EMBP1, CLO5A1, EHF, ELF5 as dominate hot spots. Taken clinical outcome into account, we found a significant difference between HPV integration occurrence and VaIN(p = 0.011). HPV integration could serve as a predictive marker for monitoring and management of VaIN in a way.
Non-stationary associations between rainfall intensity and large-scale variability in an east-Andean city over the last 108-years
Authors: Medina Franco D. , Zossi Bruno S. , Elias Ana G. 
Published: July 2023

Abstract
This work explores for the 1912–2019 period the interannual associations between the maximum daily rainfall (Rx1) in San Miguel de Tucumán (Argentina, 26.8°S, 65.2°W) and the large-scale variability. The flood hazard in this east-Andean city is high due to the presence of lowlands and urban stream channels with poor maintenance. We analyzed the interannual relation between Rx1 and El Niño Southern Oscillation (ENSO) and the Southern Annular mode (SAM), and the non-stationarity of these associations as a response to Pacific Decadal Oscillation (PDO) and Atlantic Multidecadal Oscillation (AMO). We compute the moving correlation for all possible window lengths between Rx1-ENSO and Rx1-SAM. As a new approach we based our analysis on the periods with most significant correlations (i.e., with minimum p-value). The aim was to obtain a period when there exists an association with maximum statistical confidence. Results suggest that Rx1 variability shows the highest statistical significance in its association with ENSO for the 1945–1974 period and with SAM for the 1974–2007 period. The overall non-stationary nature in the relations can be attributed to the phase shifts of PDO. Negative PDO and positive ENSO phases favor higher Rx1 values, while this also occurs with both positive PDO and SAM. Inverse phases favor lower Rx1 values. On the other hand, AMO phases do not influence relationships. Results highlight that processes involved in the variability of rainfall intensity are partly related to global variability modes, opening opportunities to enhance predictions in relation to the flood hazard.
Stability Indicating Method Development and Validation for Anticoagulant Drug Apixaban in Apixaban Drug Substance and Drug Product
Authors: Suryawanshi Shriram Pandurang , Barabde Gayatri 
Published: July 2023

Abstract
The aimed of this research is to develop the method on reverse phase liquid chromatographic (RP-HPLC) for the assay determination of Apixaban in both drug substance and tablet dosage form. A Inertsil ODS C18 (5 μm particle size, 150 mm x 4.6 mm). was used for the RP-HPLC separation of Apixaban. The mobile phase consisted of a buffer solution pH 4 and methanol in a ratio of 50:50 v/v. The flow rate was set at 1.2 ml/minute, with an injection volume of 10 μL. The column oven temperature was maintained at 40°C, while the autosampler temperature was set to 25ºC. Detection of Apixaban was performed at a wavelength of 230 nm. The developed stability indicating analytical method underwent to validation in accordance with ICH guidelines.
Results: The chromatographic condition, results showed that the peak retention time of Apixaban was 2.2 min. The linearity of Apixaban was observed in the concentration range of 7.5 µg/mL to 37.5 µg/mL, with a percent accuracy was observed in ranging from 100.0% to 101.2%. Stress testing was conducted under acidic, basic, and oxidation conditions. The linearity correlation coefficient and system suitability criteria met the requirements specified by ICH guidelines.
Conclusions: The developed RP-HPLC method exhibited linearity and accuracy, with correlation coefficients and percent recovery falling within the acceptance criteria.The method proved to be simple, rapid, stability-indicating and cost-effective for the determination of Apixaban in both drug substance and tablet form.
QL-PPTS:A Machine Learning Scheduling Scheme on Edge Computing Environments
Authors: Lu Weifeng , Wan Jiahuan , Djigal Hamza , Xu Jia , Xu Lijie 
Published: July 2023

Abstract
Multiple Access Edge computing (MEC) is a computing model that can extend cloud computing and storage capabilities to the edge of cellular networks, close to IoT devices. MEC enables computation-constrained IoT devices to execute computation-intensive or low-latency applications at the edge of the network. However, in order to execute these applications efficiently, a reasonable scheduling strategy is required. Because of the particularity of edge environment, the traditional scheduling algorithm is not suitable. In this paper, we propose a new scheduling algorithm called QL-PPTS that combines reinforcement learning with a look-ahead list scheduling algorithm PPTS to reduce the makespan. we use QL to regard the rank of predictive cost matrix (rankP CM) of PPTS as the immediate reward. After the convergence of the Q-table, the priority queue of tasks is obtained according to the greedy principle. Experiments show that the QL-PPTS algorithm outperform the QL-HEFT.Specially, QL-PPTS can outperform QL-HEFT by 9% in terms of efficiency.
Long term outcome of Uniport versus Multiport Video-Assisted Thoracoscopic Lobectomy for Non-small Cell Lung Cancer: A Propensity-Matched Study
Authors: Ruan yingding , Cao Wenjun , Xue Hongsheng , You Maoduan , Zhao Zhilong 
Published: July 2023

Abstract
Background:Uniportal Video-Assisted Thoracoscopic Surgery (U-VATS) has emerged as a feasible approach for treating non-small cell lung cancer (NSCLC). However, there is a lack of studies reporting the long-term survival outcomes of U-VATS lobectomy for NSCLC.
Objective:This study aims to compare the perioperative outcomes and long-term survival of U-VATS lobectomy for NSCLC with multiportal VATS (M-VATS, involving two ports or more) lobectomy.
Method: A total of 339 patients who underwent intentional VATS lobectomy for NSCLC between 2012 and 2017 were included in the analysis. Perioperative outcomes and long-term survival were evaluated. Propensity score matching was utilized to minimize baseline characteristic differences between the two groups.
Results:Out of the total cases, 17 (5.01%) were converted to open thoracotomy. The conversion rates were 4.96% (7/141) in the U-VATS group and 5.05% (10/198) in the M-VATS group. A total of 322 consecutive patients underwent VATS lobectomy and mediastinal lymphadenectomy. After propensity matching, 106 pairs were obtained, consisting of 83 males and 129 females. Intraoperative bleeding volume, number of retrieved lymph nodes, explored nodal stations, drainage time and volume, and postoperative hospital stay were similar between the two groups. Both groups exhibited comparable morbidity and mortality rates. From the multivariable analysis, there was no significant difference observed in terms of overall survival (OS) and disease-free survival (DFS) between the two patient cohorts.
Conclusions: U-VATS demonstrated comparable perioperative outcomes and long-term efficacy to M-VATS. However, further confirmation of these findings is required
Application and Exploration of TPKCEE Teaching Mode based on Narrative Nursing in Ideological and Political Education of Obstetric and Gynecological Nursing: a Mixed Research Study
Authors: Yu Xin-xia , Song Si‑meng , Pei Qin , Yan Feng‑xia , Yang Qiao‑hong , Tian Xiao‑ying 
Published: July 2023

Abstract
Background
As the field of nursing science continues to evolve and higher education expands, enhancing empathy and humanistic care skills among nursing students prior to their clinical practice has become a crucial responsibility for nursing educators. To investigate the impact of the TPKCEE compound teaching model, which is based on narrative nursing, on the cultivation of empathy and humanistic care qualities among students in the ideological and political teaching of obstetrics and gynecology nursing.
Methods
A convergent mixed method design was used, which consisted of a quasi-experimental design and a semistructured interview.Data collection took place from September 2021 to February 2023. A convenience sampling method was used to select 61 fulltime third-year nursing students in a school in China (a control group of 27 and an experimental group of 34). The control group was exposed to traditional curriculum, while the experimental group used the TPCKEE teaching model based on narrative nursing. Jefferson Empathy Scale (JSE) and Caring Ability Scale (CAI) were used for quantitative assessment before and after the course, descriptive statistics and Mann-Whitney U test were used to compare the empathy and caring ability of the two groups of students, and semi-structured interviews were used for qualitative assessment. Thematic analysis was used to understand the development of students in humanistic care, empathy and learning experience.
Results
After teaching, the total score of empathy and the dimension of transposition, emotion and viewpoint, and the total score of CAI and the dimension of understanding and courage of nursing students in the control group were better than those in the control group (P < 0.05).
Conclusion
The TPCKEE teaching model based on narrative nursing effectively improves empathy, caring behavior, and classroom teaching outcomes in third-year nursing students.