bluuebunny/crossref_metadata_2025_split huggingface dataset:
Removal of Osseointegrated Implants: Surgical Techniques and New Approaches, a Systematic Review
Authors: Tafuri Giuseppe , D'Addazio Gianmaria , Manciocchi Eugenio , Rexhepi Imena , Santilli Manlio , Caputi Sergio , Sinjari Bruna 
Published: August 2023

Abstract
Background
Today dental implants represent an effective therapy in case of partial or total edentulism, with an excellent success rate. Despite the results obtained, there may be biological or mechanical complications during the therapy, which lead to the loss of the implant. This systematic review aims to evaluate the current state of the art in the literature on techniques used for the removal of dental implants. Various aspects will be analyzed, such as the success of the technique, any complications, and the advantages and disadvantages of their use.
Methods
Two reviewers conducted a literature analysis (PubMed, Embase, Web of Science) of the last 20 years (2003–2023). The main criterion analyzed was the success of the technique, while secondary outcomes such as complications and risks of the technique were also analyzed. 258 articles were identified in the various search databases. 42 eligible articles were subsequently identified after an article screening. Only 18 full texts were subsequently included in the review.
Results
A total of 18 articles were selected and 1142 implants and 595 patients were included. The main techniques used were the Counter-Torque Ratchet Technique (CTRT), Piezosurgery, trephine drills, carbide burs, Erbium, Chromium, Yttrium, Scandium, Gallium, Garnett (Er:Cr:YSGG) laser and carbon dioxide (CO2) laser. Combined uses of techniques have been identified such as: Piezosurgery and trephine burs or carbide burs, trephine burs with the use of a 3d-printed-guide, CTRT and trephine burs. The technique with the highest success rate, less morbidity for the patient, and less removal of bone appears to be the CTRT.
Conclusions
The use of conservative techniques, especially CTRT, in bone removal is useful to allow for immediate implant placement in the removal area. However, further studies with a high sample size are needed to be performed on all techniques, particularly new RCTs that allow for the analysis of the success of alternative techniques such as Laser and Piezosurgery, which appear to be very promising.
Habitual smoking and dental health: a cross-sectional study of the National Health and Nutrition Examination Survey (NHANES) 2011-2018
Authors: Dang Xiangji , Liu Guanchen , Zhao Zhihu , Mu Lijuan , Yang Ruifeng , Qi Jing , Niu Qianqian , Liu Yan 
Published: August 2023

Abstract
Background: Smoking can damage dental health, but the relationship between the duration of habitual smoking and the degree of dental damage has not been reported.
Objective: This research aimed to investigate the dental health of habitual smokers who participated in the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2018 and to assess the relationship between the duration of regular smoking and dental health.
Methods: Participants in the NHANES database aged ≥18 and ≤70 years were included in this study. Based on the responses to the smoking questionnaire, the regular smoking time is estimated. Data concerning dental health were derived from the results of dental screenings. This study investigated the connection between the average smoking time of the participants and dental health using a multivariate linear regression model. This study created a risk prediction model to assess tooth injury risk in the smoking population.
Results: A total of 3113 participants with complete information were involved. The analysis of multiple linear regression models showed that the longer a person smoked, the more severe the damage to their teeth, with a smoking year of 20 increasing to 0.069 (β=0.069，95% CI (0.024, 0.114), p<0.0001) and a smoking year of more than 20 rising to 0.135 (β=0.135, 95% CI (0.070,0.201), p<0.0001). The smokers who had been smoking for more than 20 years had a 2.7-fold higher number of unhealthy teeth than the group who had smoked for less than 20 years (β=2.7, 95% CI (2.2, 3.3), p<0.0001).
Conclusion: The longer smokes, the more detrimental the impact on dental health can be. Therefore, smoking can negatively affect teeth, making it beneficial to quit or reduce smoking as soon as possible for better dental health. Several tools have been developed include prediction curves, risk assessment models, and assessment scales to assess the dental status of smokers and promote better dental health.
Interleukin-6 Blockade With Tocilizumab In Idiopathic Multicentric Plasma Cell Variant Castleman Disease: Case report
Authors: Naranjo-Saltos Fernando , Lescano Martin , Arce Yanael , Fernandez Heidi Angela 
Published: August 2023

Abstract
Introduction: Castleman disease represents a group of rare lymphoproliferative disorders with heterogeneous manifestations and similar histopathological features. Due to its broad spectrum of clinical presentations, it can be challenging to reach an accurate diagnosis promptly, thereby delaying adequate treatment for patients. In certain circumstances, demographic and socioeconomic factors influence the treatments patients receive. We aimed to describe the initial response of a patient with idiopathic Multicentric Castleman Disease (iMCD) to treatment with Tocilizumab. Case Presentation: We present the case of a patient with a year-long history of unexplained anemia and, most recently, lymphadenopathy. After a thorough review of systems and extensive workup, a diagnosis of idiopathic multicentric Castleman disease was made. Treatment with tocilizumab, an anti-Interleukin-6 receptor antibody, was initiated due to its availability in countries with limited resources, such as ours. The patient had a rapid favorable clinical response with no signs of relapse. Conclusions: Castleman disease is a lymphoproliferative disorder that should be on our radar when dealing with the differential of a patient with localized or generalized lymphadenopathy. The pathogenesis of iMCD involves hyperproduction of Interleukin 6 (IL-6), and there has been significant progress in its management with monoclonal antibodies, specifically those that involve IL-6 blockade. Anti-IL-6 therapy with tocilizumab can be an option for iMCD treatment, especially in countries where other first-line options are not as readily available.
Identification of pre-determined steroids in commercially available dietary and traditional supplement products in South Africa
Authors: Gabriels Gary , Lambert Michael , Smith Peter 
Published: August 2023

Abstract
Background
An age-old fable can be traced as far back to third century BC that shows ‘sport supplements’ being used by athletes of that era, who made use of ‘substances’, with the aim to improve performance. In more recent times, subsequent to the reunification of Germany, ground-breaking findings were found when acquiring a number of highly classified scientific documents and reports. These documents revealed the systematic state-sponsored programmes of doping of athletes. Specifically linked to anabolic androgenic steroids being a controlled substance globally in several countries. Amidst this ‘control’, steroids are generally readily available for non-medicinal purposes, including for body-image use. Further, in modern day sport and wellness practice, dietary supplements have become of common use and widespread, for competitive and recreational athletes of all ages. This has contributed to modern manufacturing innovation having supplements products that potentially may include illicit substance(s). These often ‘designer steroids’ may be manufactured specifically, to circumvent sport anti-doping laboratory tests, in human sport, and therefore supplied in a clandestine fashion.
Aim
The research study investigated the industry associated with commercially available dietary and traditional supplements. In particular the research assessed the content of these products for prohibited substances, with reference specifically to pre-determined steroids, that may not necessarily be declared on the product label.
Methods
A total of 138 dietary supplements products formed part of the assessment. The products were laboratory analysed for 16 pre-determined steroids, using Tandem Liquid Chromatography Mass Spectrometry.
Results
The frequency of positive tested samples for the respective steroids assessed in the overall cohort ranged from 24–86%, for South African produced dietary supplements 15–89%, and for imported products, bought in South Africa, 12–90%.
Conclusion
The findings of the steroid positive tested samples provide evidence for implementing an improved system for regulating, monitoring, and enforcing the quality control of dietary supplements.
Bioinformatics analysis of ceRNA network of autophagy-related genes in childhood asthma
Authors: Zhu Hao , Shi Jiao , Liao Qing , Xu Biao 
Published: August 2023

Abstract
Background
The key differentially expressed autophagy-related genes(DE-ARGs) in childhood asthma were screened, and lncRNA-miRNA-mRNA networks were constructed to further understand the pathogenesis of asthma.
Methods
DE-ARGs were identified using the Gene Expression Database (GEO) and the Human Autophagy Database. These DE-ARGs were subjected to Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway, Gene Set Enrichment Analysis, and protein-protein interaction network analysis followed by further verification of core gene expression. MiRNAs were inversely predicted using two databases (miRDB and ENCORI), while miRNA-lncRNA interactions were predicted using LncBase and ENCORI databases. After excluding lncRNAs present only in the nucleus and extracellular space, a competitive endogenous RNA (ceRNA) network was established and further analyzed. Finally, we validated mRNA expression levels in the ceRNA network by quantitative real-time PCR (qRT-PCR).
Results
31 DE-ARGs were obtained, of which 29 were up-regulated and two were down-regulated. Autophagy, regulation of apoptotic signaling pathways, interferon-α/β signaling, interferon γ signaling, autophagy-animal, and apoptosis pathways were mainly enriched in asthma. Five hub genes (VEGFA, CFLAR, RELA, FAS, and ATF6) were further analyzed to verify the expression and diagnostic efficacy of these core genes using the GEO dataset. Finally, four hub genes (VEGFA, CFLAR, RELA, and FAS) were obtained. Through the combination of literature search, ceRNA network mechanism, and predicted miRNAs and lncRNAs analysis, a ceRNA network of four mRNAs (VEGFA, CFLAR, RELA, and FAS), three miRNAs (hsa-miR-320b, hsa-miR-22-3p, and hsa-miR-625-5p), and 35 lncRNAs was finally constructed. qRT-PCR showed that FAS was signifcantly upregulated.
Conclusion
Four DE-ARGs, especially FAS, may play a key role in asthma. The new ceRNA network may help to explore the mechanism of autophagy in asthma, which may be important for the development of new treatment options.
Analysis of m7G-Related Signatures in Adrenocortical Carcinoma and Their Correlation with Survival
Authors: lin junhan , Huang Chaoqun , Hu Ende , Chen Yaozhi , Lin Chuanqi 
Published: August 2023

Abstract
N7-methylguanosine (M7G) is a prevalent modification of mRNA in biological systems, and plays a role in various biological processes. Previous research has demonstrated that expression of m7G RNA modification is correlated with cancer and a range of other pathological conditions. The study aimed to explore the potential of m7G as a prognostic biomarker and therapeutic target for Adrenocortical Carcinoma (ACC). A comprehensive analysis was conducted to identify m7G-related genes in ACC by first compiling a list of 26 critical regulators through previous research and Gene Set Enrichment Analysis (GSEA). Subsequently, LASSO Cox regression analysis was performed on RNA-seq data from The Cancer Genome Atlas (TCGA) and accompanying clinical data, resulting in the identification of nine m7G prognostic signatures (GEMIN5, DCPS, AGO2, EIF4E2, NCBP1, WDR4, EIF4A1, EIF4E3, NUDT16) to create a predictive signature. Patients with ACC were then classified into high- and low-risk groups based on the predictive signature, with the results showing that patients in the high-risk group had a poorer prognosis. The m7G signature demonstrated high diagnostic sensitivity and robustness, as demonstrated by its diagnostic performance and external validation through the Gene Expression Omnibus (GEO). This study provides a comprehensive analysis of m7G RNA methylation in ACC and offers insight into the gene expression, function, interaction, and predictive value of m7G-related genes, which may provide valuable information for prognosis prediction and treatment guidance for ACC patients.
Turning anecdotal irradiation-induced anti-cancer immune responses into reproducible in situ cancer vaccines via disulfiram/copper-mediated enhanced immunogenic cell death of breast cancer cells
Authors: Wang Xinhui , Guo Wei , Jia Lin , Xie Ling , Kiang Juliann , Wang Yangyang , Wang Fuyou , Lin Zunwen , Wang Enwen , Zhang Yida , Huang Peigen , Sun Ting , Zhang Xiao , Bian Zhengying , Tang Tiejun , Guo Jingtian , Ferrone Soldano 
Published: August 2023

Abstract

Irradiation (IR) induces immunogenic cell death (ICD) in tumors, but it rarely leads to the abscopal effect (AE). However, combining IR with immune checkpoint inhibitors has shown anecdotal success in inducing AEs. In this study, we aimed to enhance the IR-induced immune response and generate reproducible AEs using the anti-alcoholism drug disulfiram (DSF) and copper complex (DSF/Cu) via induction of tumor ICD. We measured ICD in vitro and in vivo. In mouse tumor models, DSF/Cu was injected intratumorally followed by localized tumor IR, creating an in situ cancer vaccine. We determined the anti-cancer response by primary tumor rejection and assessed systemic immune responses by tumor rechallenge and the occurrence of AEs, i.e., spontaneous lung metastasis. Additionally, we analyzed immune cell subsets and quantified proinflammatory and immunosuppressive chemokines/cytokines in the tumor microenvironment (TME) and blood of the vaccinated mice. Immune cell depletion was investigated for its effects on the vaccine-induced anti-cancer response. The results showed that DSF/Cu and IR induced more potent ICD under hypoxia than normoxia in vitro. Low-dose intratumoral injection of DSF/Cu and IR demonstrated strong anti-primary and -rechallenged tumor effects and robust AEs in mouse models. These vaccinations also increased CD8 + and CD4 + cell numbers while decreasing Tregs and myeloid-derived suppressor cells in the 4T1 model, and increased CD8+, DC, and decreased Treg cell numbers in the MCa-M3C model. Depleting both CD8 + and CD4 + cells abolished the vaccine's anti-cancer response. Moreover, vaccinated tumor-bearing mice exhibited increased TNFα levels and reduced levels of immunosuppressive chemokines/cytokines. In conclusion, our novel approach generated an anti-cancer immune response, resulting in a lack of or low tumor incidence post-rechallenge and robust AEs, i.e., the absence of or decreased spontaneous lung metastasis in tumor-bearing mice. This approach is readily translatable to clinical settings and may increase IR-induced AEs in cancer patients.
Sight Impairment registration in Trinidad: 35-year trend in causes and population coverage in comparison to the National Eye Survey of Trinidad and Tobago
Authors: Ramsewak Shivaa , Deomansingh Frank , Winford Blaine , Bartholomew Debra , Maharaj Vedatta , Fraser Amandi , Singh Deo , Suratt Kenneth , Tripathi Vrijesh , McNally Kevin , Sharma Subash , Bascaran Covadonga , Ramsewak Samuel , Bourne Rupert , Braithwaite Tasanee 
Published: August 2023

Abstract
Background: Little was known causes and coverage of sight impairment (SI) registration in the Caribbean. We compared the Trinidad and Tobago Blind Welfare Association (TTBWA) register and findings from the 2014 National Eye Survey of Trinidad and Tobago (NESTT).
Methods: This study included TTBWA register analysis; cross-sectional validation studies of registered clients, involving interviews, vision function and cause ascertainment in July 2013, and interview and visual function measurement only in July 2016; comparison of causes of SI between register and national survey, and estimation of registration coverage.
Results: The TTBWA register included 863 people (all ages) registered between 1980 and 2015, 48.1%(n=415) male. The NESTT identified 1.1%(75/7158) people aged >5years eligible for SI or SSI registration, 49.3%(n=37) male. The causes of SI and SSI agreed closely between the register and population-representative survey, with glaucoma being the leading cause in both the register (26.1%,n=225) and population survey (26.1%, 18/69 adults), followed by cataract and diabetic retinopathy. In the validation studies combined, 62.6%(93/151) clients had SSI, 28.5%(43/151) had (partial) SI and 9.9%(15/151) did not meet SI eligibility criteria. Registration coverage was approximately 7% of the eligible population in Trinidad. SI and SSI were potentially avoidable in at least 58%(n=36/62) adults and 50%(n=7/14) children examined in the 2013 validation study.
Conclusion: We report close agreement in causes of SI between a national register and contemporaneous national population-based eye survey, but highlight low register coverage, and that at least half of all SI resulted from preventable, treatable or curable eye diseases.
Comparison of pregnancy outcomes in two methods of frozen and fresh embryo transfer in infertile women with endometriosis
Authors: Hosseinimousa Sedigheh , Talebian Marzieh , Piri Masoumeh , Pourmahmoudian Reza 
Published: August 2023

Abstract
Introduction: infertility is one of the most important complications in patients with endometriosis however, the use of assisted reproductive treatments have resulted in successful fertility and childbearing in a significant proportion of patients. In this study the outcome of pregnancy was compared between two methods of frozen and fresh embryo transfer in infertile women with endometriosis who underwent assisted reproductive treatment in Shariati Hospital from 2013 to 2020.
Methods: From 197 patients with endometriosis who received infertility treatment, 105 were assigned to fresh embryo transfer group and 92 were assigned to frozen embryo transfer group. Patient’s data was gathered using archive files. Patients in both groups were matched in terms of age, BMI (Body Mass Index), duration of primary infertility, base FSH (Follicle-Stimulating Hormone) serum levels and number of transferred embryos (P>0.05). The IVF (In Vitro Fertilization) pregnancy outcomes in two groups were compared in terms of live birth rate, clinical pregnancy rate, chemical pregnancy rate, miscarriage rate, ongoing pregnancy rate.
Results: Live birth rate, clinical pregnancy rate, ongoing pregnancy rate and chemical pregnancy rate in frozen embryo transfer group was significantly higher than fresh embryo transfer group (P<0.049). miscarriage rate was not significantly different in both groups (P=0.546).
Conclusion: According to the results and parameters in this study frozen embryo transfer is more successful in terms of pregnancy outcomes than fresh embryo transfer.
Alcohol use, mental health status, and alcohol-related problems in university students amid COVID-19 pandemic
Authors: Chau Siu Long , Wong Yiu Cheong , Wu Yongda , Wang Man Ping 
Published: August 2023

Abstract

Background
Alcohol is widely used by university students worldwide and associated with mental health problems, but little is known about its prevalence amid the COVID-19 pandemic, particularly in Asian regions. We aimed to investigate alcohol use, mental health status, and alcohol-related problems in university students amid the COVID-19 pandemic.
Methods
In this secondary analysis of a randomized controlled trial study, we recruited students with Alcohol Use Disorder Identification Test (AUDIT) ≥ 8 from 10 local universities during the pandemic (2020–2022) in Hong Kong. Alcohol-related problems were measured by the Academic Role Expectations and Alcohol Scale (AREAS). Anxiety and depressive symptoms were measured by General Anxiety Disorder-2 (GAD-2) and two-item Patient Health Questionnaire (PHQ-2). Socio-demographic and alcohol-drinking behaviors were also assessed. Adjusted unstandardized coefficients (b) for AREAS score and odds ratio (AOR) for the academic problems were calculated, and interactions by mental health status were assessed.
Results
Of 772 participants (mean age: 21.2, 47.9% male), the mean AREAS score was 2.4 (SD 3.2) and 68.2% had underage drinking. 69.3% binge drank and 28.9% had heavy drinking in the past 30-day, and 38.0% reported increased drinking amid the COVID-19 pandemic. 28.3% and 28.8% had anxiety and depressive symptoms, respectively. Higher drinking frequency, binge drinking, heavy drinking, anxiety, and depressive symptoms were associated with higher AREAS scores (adjusted b range 0.78–1.85, all P < 0.05). Binge drinking was associated with late for class, class absenteeism, class inattention, and late assignment submission (AORs range 1.75–3.89, all P < 0.05). Participants with anxiety or depressive symptoms had stronger associations of higher drinking frequency (anxiety: b 1.72 vs. 1.03; depression: 2.47 vs. 0.83), binge drinking (anxiety: b 2.77 vs. 1.73; depression: 2.94 vs. 1.63), and heavy drinking (anxiety: b 2.42 vs. 1.17; depression: 2.61 vs. 1.27) with AREAS scores (all P for interactions < 0.05).
Conclusions
University students had a high prevalence of binge drinking, heavy drinking, and mental health problems amid the COVID-19 pandemic. Alcohol use in the pandemic was associated with multiple alcohol-related problems, particularly in university students with mental health problems. Interventions are warranted to reduce alcohol use and related harm in the future.
Defects and residual stresses Finite Element prediction of FDM 3D printed Wood/PLA biocomposite
Authors: Morvayová Alexandra , Contuzzi Nicola , Casalino Giuseppe 
Published: August 2023

Abstract
The rising interest amongst research community in utilizing PLA-based biocomposites for Fused Deposition Modelling (FDM) is driven by the increasing demand for eco-friendly and cost-effective materials for various applications. While significant progress has been made in understanding the process-structure-property relationship, the intricate interconnections involved in this context remain only partially revealed. Current lack of knowledge poses challenges in achieving optimal quality and dimensional accuracy of FDM-manufactured specimens from biocomposites. Although numerous numerical models exist for simulating the FDM process, there is a distinct need for models tailored to the specific characteristics of biocomposites. This study presents a 3D coupled thermomechanical numerical model designed to predict dimensions, defect formation, residual stresses, and temperature in PLA/wood cubes produced by FDM, considering various process parameters and composite-like nature of wood-filled PLA filaments. The accuracy of the proposed numerical model was validated by comparing its results with experimental measurements of biocomposite cubes manufactured under the same process parameters. Encouragingly, the simulated dimensions showed a maximum relative error of 9.52% when compared to the experimental data, indicating a good agreement. The numerical model also successfully captured the defect formation in the manufactured cubes, demonstrating consistent correspondence with defects observed in the experimental specimens. Moreover, the study highlighted the influence of wooden additives on defect formation.