bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue culture and rapid propagation technology for Gentiana rhodantha
Authors: Han Suju , Liu Mei , Wang Yuetong , Chen Juan 
Published: May 2023

Abstract

Gentiana rhodantha is a perennial herb of the genus Gentiana (Tourn.) L. This study was novel in establishing a regeneration system of G. rhodantha using young leaves as explants on the Murashige and Skoog (MS) medium supplemented with different plant growth regulators (PGRs). The roots, stems, and leaves of G. rhodantha were used as explants. The effects of the optimal explant disinfection method, type of explant used, concentrations of PGRs added to the culture media on tissue culture, and rapid propagation of G. rhodantha were studied. The results showed that the optimal disinfection method for stems and roots consisted of disinfection using 75% ethanol for 50 s, followed by 4% sodium hypochlorite (NaClO) for 10 min. The optimal disinfection technique for leaves consisted of disinfection using 75% ethanol for 50 s, followed by 4% NaClO for 8 min. Root explant was the most suitable for inducing the callus of G. rhodantha on the MS medium supplemented with different PGRs. The optimal conditions for callus induction included 1.0 mg/L 6-benzylaminopurine (6-BA) and 0.5 mg/L α-naphthalene acetic acid (NAA). The callus induction rate using the root explant reached 94.28%. MS supplemented with 2.0 mg/L 6-BA and 0.1 mg/L NAA was the optimal medium for inducing adventitious shoots from the callus of G. rhodantha. The best medium for propagation and plantlets strengthening was MS supplemented with 0.8 mg/L 6-BA and 0.3 mg/L NAA, and the propagation index was 8.62. MS supplemented with 0.3 mg/L 3-indolebutyric acid was the best culture medium for inducing the rooting of adventitious buds, with the maximum rooting rate reaching up to 100%.
“A new worker, for a new order, in a new era”: English, power and shifting ideologies of reflexivity in a Chinese global workplace
Authors: Gong Eleanor Yue 
Published: May 2023

Abstract
This paper offers a historiographic and ethnographic analysis of how reflexivity, as a communicative practice and valued personality trait, has been understood, regulated, legitimised and used to control Chinese workers from the planned-economy era to the present. Using a Shanghai-based multinational company as a case study, I document how and under what conditions English-mediated reflexivity, with its stress on self-entrepreneurship, came to replace former Mandarin-mediated reflexivity supporting a notion of collective workerhood. Special attention is paid to reflexivity’s changing roles in shaping, managing and evaluating workers and facilitating understandings of labour, power and agency. The paper argues that the emerging English-dominated reflexivity represents a required linguistic shift for the creation of a new worker type in the current globalised economy as it normalises managerial technologies of discipline, stratification and exclusion.
Aminosilyl-substituted cyclopentadienyl complexes of alkali metals
Authors: Mohamad Media , Lambert Jessica , Wirtz Lisa , Morgenstern Bernd , Schäfer André 
Published: May 2023

Abstract
(Pyrrolidinyldimethylsilyl)tetramethylcyclopentadienide complexes of lithium, sodium and potassium were synthesized and characterized, including crystal structure determinations. The lithium cyclopentadienide compound was used as a Cp transfer reagent to prepare the corresponding ferrocene, stannocene and plumbocene.
Development of Temperature-Controlled Direct Shear Box
Authors: Emami Ahari Hossein , Ajmera Beena 
Published: May 2023

Abstract
An increase in the temperature of permafrost that is caused by global warming can lead to a significant decrease in shear strength. Seasonal freeze-thaw (F-T) cycles can also adversely affect the shear strength of soils. This can result in damages to infrastructure, negative impacts on the economy, and a decline in the quality of life. Thus, it is crucial to understand the shear strength of permafrost and seasonally frozen-thawed soils. Several studies have utilized various instruments to observe the behavior of soils under such conditions, including a temperature-controlled triaxial system to apply F-T cycles or a traditional direct shear apparatus placed within a temperature-controlled room. Since most commercial geotechnical labs do not have a temperature-controlled room or a temperature-controlled triaxial system, this article presents the design of a new cost-effective direct shear box that was developed to allow temperature-controlled testing in a traditional direct shear device. The modifications to the direct shear box comply with ASTM D3080/3080M, Standard Test Method for Direct Shear Test of Soils under Consolidated Drained Conditions. Like the standard direct shear box, it consists of two halves and a direct shear cap, but each of these components is hollow to allow for the circulation of glycol. The chiller is capable of imposing temperatures within the range of −40°C to +40°C on the sample being tested. It is also possible to freeze and thaw specimens at a desired normal stress while monitoring the associated heave and compression. The freezing mechanism applied to the soil sample affects the distribution of ice within the pore spaces, necessitating that samples be frozen from all sides if a uniform distribution of ice is necessary. Shear strength parameters from the newly designed temperature-controlled direct shear box matched well with those from the traditional shear box. In addition, the feasibility of temperature-controlled direct shear testing was evaluated at different temperatures, strain rates, and normal stresses.
Endothelial Cells Are Heterogeneous in Different Brain Regions and Are Dramatically Altered in Alzheimer's Disease
Authors: Bryant Annie , Li Zhaozhi , Jayakumar Rojashree , Serrano-Pozo Alberto , Woost Benjamin , Hu Miwei , Woodbury Maya E. , Wachter Astrid , Lin Gen , Kwon Taekyung , Talanian Robert V. , Biber Knut , Karran Eric H. , Hyman Bradley T. , Das Sudeshna , Bennett Rachel E. 
Published: May 2023

Vascular endothelial cells play an important role in maintaining brain health, but their contribution to Alzheimer's disease (AD) is obscured by limited understanding of the cellular heterogeneity in normal aged brain and in disease. To address this, we performed single nucleus RNAseq on tissue from 32 human AD and non-AD donors (19 female, 13 male) each with five cortical regions: entorhinal cortex, inferior temporal gyrus, prefrontal cortex, visual association cortex, and primary visual cortex. Analysis of 51,586 endothelial cells revealed unique gene expression patterns across the five regions in non-AD donors. Alzheimer's brain endothelial cells were characterized by upregulated protein folding genes and distinct transcriptomic differences in response to amyloid β plaques and cerebral amyloid angiopathy. This dataset demonstrates previously unrecognized regional heterogeneity in the endothelial cell transcriptome in both aged non-AD and AD brain.SIGNIFICANCE STATEMENTIn this work, we show that vascular endothelial cells collected from five different brain regions display surprising variability in gene expression. In the presence of Alzheimer's disease pathology, endothelial cell gene expression is dramatically altered with clear differences in regional and temporal changes. These findings help explain why certain brain regions appear to differ in susceptibility to disease-related vascular remodeling events that may impact blood flow.
Effects of mineral supplementation on qualitative beef parameters
Authors: Farionik T. V. , Yaremchuk O. S. , Razanova O. P. , Ohorodnichuk G. M. , Holubenko T. L. , Glavatchuk V. А. 
Published: May 2023

Correct nutrition with sufficient amount of optimal doses of microelements efficiently supports high levels of beef production and health of the animals throughout the feeding period. In the sphere of beef production, microelements are needed as additional supplements to play an important role in the productivity of cattle and the quality of the products made from them. Providing cattle with a more bioavailable source of deficient microelements could increase the metabolic process of the main nutrients, which would affect the intensity of bull growth and slaughter meat parameters. It has been revealed that organic complexes of deficient microelements have greater bioavailability. Throughout the experiment, Ukrainian Black Spotted bulls of 175–200 kg live weight were fed with diets enriched by chelate compounds of microelements with lysine and methionine. Animals of the second group were fed a diet with methionates of microelements in the following doses, mg/kg of live weight: iron – 0.05, cobalt – 0.04, iodine – 0.05 and selenium – 0.02; the third group received lysinates in the same doses of microelements; and the fourth group was given lysinates and methionates in a complex with half-doses of microelements. The studies were carried out using the following methods: zootechnical (productivity), biochemical (chemical blood composition), morphological (weight and sizes of certain tissues and internal organs), physical-chemical and sanitary meat parameters and statistical (mean arithmetic value and its error, significance level of difference between parameters). We found evidence and confirmed the benefits of using microelement supplemention with essential aminoacids in the bulls’ diet. Feeding chelate supplement to animals during the second feeding period improved hematological parameters, the productivity and nutrition value of beef. We determined that the best outcomes resulted from feeding bulls with lysinates and methionates in complex with microelements (Fe, Co, Se and I) during the second feeding period. The animals had 26.0% higher mean daily increments and 11.1% greater growth rates, and they grew 25.8% more intensively than the others that consumed only the main diet. The animals of this group had 5.2% higher slaughter yield and 4.8% higher carcass yield. The experimental groups were observed to have lower number of microorganisms in the longissimus layer in one field of view when analyzing impression smears. After 14 days of beef storage, qualitative reactions in the control group were positive with formaldehyde, sulphuric-acid copper, Nessler’s reagent and negative with benzidine. The reactions in the samples from experimental groups were doubtful. Less deterioration of beef stored at low positive temperatures (0…+2 ºС) was observed for the samples of experimental groups.
Diversity of acarine fauna (Acari: Parasitiformes, Acariformes) inhabiting ornamental trees and shrubs in Hungary: A review
Authors: Ripka Géza 
Published: May 2023

AbstractThis paper reviews of the recent progress in the acarological researches and knowledge on mites occurring in the canopy of ornamental trees and shrubs. The inventory of Acari includes 444 mite species belonging to 33 families which were recorded from 283 hardwood tree and shrub species of 45 plant families. The highest number of mite families and species were reported from the families Rosaceae (21 mite families), Salicaceae (20) and Oleaceae (17), and Salicaceae (99 mite species), Rosaceae (98) and Fagaceae (63), respectively. The most diverse acarine fauna was found on Acer campestre (37 mite species), Prunus spinosa (37), Acer pseudoplatanus (33), Tilia cordata (33) and Salix alba (31). The most frequently occurring mite species were Euseius finlandicus (on 78 host species), Tydeus californicus (64) and Zetzellia mali (60).
Implementasi Sabar dalam Mendidik Anak Tunagrahita
Authors: Syifani Cindhea 
Published: May 2023

This study aims to identify the implementation of patience of teachers at SLB-C Silih Asih in educating mentally retarded children. This research is a type of field research using a qualitative approach, which places the researcher as the core instrument, in which the result of the study will be greatly influenced by the ability of the researcher to analyze and interpretation. Interviews and observation were used as data collection techniques so that the data obtained were more comprehensive. This study found that teachers at SLB C Silih Asih are also patient individuals and implement patience in educating mentally retarded children. The methods used by the teacher include the method of communication, simulation, practice and prompts. This method serves to catch up with various delays form mentally retarded children. The use of this method will certainly be more effective if it is realized patiently. Based on the result of interviews, the method has been carried out with great patience in order to achieve the expected goals. Based the research findings, it was concluded that the subjects were patient individuals and had applied in the learning process.
Ethics Toward Nature in View of Seyyed's Ecosophy Hossein Nasr
Authors: Fauzi Ramdani , Haidar Muhammad 
Published: May 2023

This research aims to discuss the environmental crisis that occurs almost all over the world. This research uses a library research approach that contains descriptions of theories, findings and research materials obtained from reference materials to make the basis of research. The results of this study show that the cause of global environmental damage is due to the influence of the reductionist and exploitative western scientific paradigm. Descartes' philosophy that separates the spirit from the body and Newton's mechanistic physics that considers every organism can be known by separating it into small parts like a machine make science dry of spiritual things. Humans are an integral unit and cannot be separated from this nature, awareness of the position of humans in nature as caliphs who must maintain and preserve nature is the main key in handling this natural damage. Nasr provides a solution with Scientia Sacra, which is sacred knowledge that involves revelation which is the heart of knowledge and it is the center of this environment that includes and determines tradition.
Accounting for heat release in small volumes of matter on the example of the growth of ZnO micro-rods: search for a modeling technique
Authors: Matyushkin I. V. , Telminov O. A. , Mikhaylov A. N. 
Published: May 2023

Using examples of an exothermic chemical reaction and self-heating of the region of a conducting filament of a memristor, heat-induced phase transitions, disadvantages of applying the classical Fourier approach on the nanoscale, and advantages of the molecular mechanics method at modeling the temperature factor are discussed. The correction for Arrhenius relationship, taking into account that the temperature becomes a random variable is proposed. Based on the introduced concepts (elementary act of heat release, distance and region of thermal impact) method for taking into account the thermal factor, is proposed.The correction is based on splitting the entire pool of particles into several, each of which corresponds to a fixed temperature value taken from a certain range. Although continuous and discrete correction options are given both, but the discrete option is more preferable. This is due to the fact that the methodology focuses on the application of methods of molecular mechanics, and, intentionally, in the most primitive version. The role of amorphization is noted as an example of the structural restructuring of matter in nano-volumes. It is indicated that the phonon spectra themselves, which determine heat transfer, depend on temperature. The technique is consistent with the ideology of multiscale modeling. The integral temperature increase is calculated outside the region of thermal exposure, where nonequilibrium effects are significant, by solving the standard equation of thermal conductivity.
Comportamientos cognitivos y afectivos del consumidor en relación con la marca
Authors: Otero Gómez Maria Cristina , Giraldo Pérez Wilson 
Published: May 2023

Objetivo: Analizar el efecto de los componentes cognitivos y afectivos en el apego a la marca/comunidad, así como también el comportamiento posconsumo a través de las variables: boca en boca positivo (Word Of Mouth-WOM+, Resistencia a la Información Negativa (RIN) e Intención de Recompra (IR) para determinar qué constructo presenta mayor influencia. Método: Estudio cuantitativo que expone un modelo causal de relaciones consumidor-marca-comunidad de marca. Las variables analizadas superaron las pruebas de validez y fiabilidad, cuya comprobación se realizó a través de la modelización de ecuaciones estructurales mediante el uso del software SmartPLS. Resultados: Se comprobó que el Apego a la Marca (AM) tiene como antecedentes directos la implicación y la identificación de marca. Además, tanto el Apego a la Marca (AM) como el apego a la comunidad de marca conllevan a la promoción de la marca, a la resistencia a la información negativa y al interés de repetir compras en el futuro. Conclusiones: Los consumidores desarrollan una relación a largo plazo con su marca, para ello, se apoyan en primer lugar en el componente cognitivo y luego en el afectivo. Además, existen categorías en las cuales el comportamiento reflexivo, analítico y consciente del consumidor es el que motiva y conduce a tomar las decisiones de compra y uso de la marca.
PREDICTION OF EMERGENCY SITUATIONS IN THE PRODUCTION OF ZEOLITE TYPE A
Authors: Vypritskova Darya V. , Sagatdinov Tagir F. , Kulakova Ekaterina S. , Grigorieva Tamara V. 
Published: May 2023

This article examines the problem of an emergency situation at work. The main causes of accidents at zeolite production facilities are described. The aim of the work is to improve the efficiency of the industrial safety and labor protection management system at the facility by analyzing incidents, identifying causes and systemic violations, finding solutions to prevent and eliminate such situations. In accordance with the goal, the following tasks were set: analysis of accidents, incidents and incidents at the considered and similar facilities, systematization and detailed analysis of technical and organizational causes and systemic violations leading to the occurrence of accidents. Based on the analysis, make recommendations on improving the effectiveness of measures to ensure industrial safety and labor protection at the facility.After analyzing a number of emergencies, it is possible to identify the causes and systemic violations. All the reasons can be divided into two groups: organizational and technical. As a result of a systematic analysis of the causes of emergencies, the primary sources of its occurrence were identified for each group of causes, and areas of activity were proposed, as a result of which, in the case of their effective application, the number of accidents significantly decreases, and the indicators of safe work at facilities increase. The main conclusions of the work: 1. it is necessary to modernize production with the involvement of financing for the renewal of outdated equipment; 2. pay special attention to the selection of qualified personnel, their training and motivation; 3. effective and high-quality management of human resources, together with the involvement of funding sources to solve technical problems, will, in our opinion, ensure an acceptable level of security. The practical significance of the work done lies in the implementation of the proposed measures to improve the efficiency of industrial safety and labor protection management.
CITRIC ACID USAGE FOR INDUSTRIAL SAFETY INCREASE
Authors: Savicheva Yulia N. , Mikheeva Daria S. , Gabdrakhmanova Rimma R. 
Published: May 2023

The problem of mineral salt deposition is one of the most significant in the operation of oil fields. Salt deposits can lead to production downtime, an emergency situation and serious threat to personnel safety. To date, there are various methods of salt formation preventing. Chemical protection methods using inhibitors have become the most widespread. Despite the successes in the application of this type of protection, the accident rate remains at a high level. In this regard, the inhibitory protection effectiveness increasing remains relevant.The article suggests the use of citric acid (CA) as a co-inhibitor of salt deposits. The purpose of this work is to study the effectiveness of citric acid usage to prevent salt deposit formation and increase the level of industrial safety of oilfield equipment.This article describes a new method of combating salt deposits and presents the results of experimental studies to assess the citric acid effect on the salt deposition inhibitor (SDI) effectiveness. SDIs have been tested for their ability to prevent the salt formation. The ISO effectiveness was determined by the trilonometric method. It has been shown that one of the inhibitors in the dosage range of 30–100 g/t has extremely low protective properties, but with the citric acid addition, its effectiveness has increased. The best results of the protective effect of 75 % were achieved using citric acid and the second inhibitor.According to laboratory test results, a positive effect of citric acid on the inhibitor effectiveness was revealed. It has been experimentally proved that citric acid can be used to protect oilfield equipment from salt deposits.
<em>Centrosema flavescens</em> (Fabaceae, Papilionoideae), a new species from the Yucatan Peninsula, Mexico
Authors: Carnevali Fernández-Concha Germán , Angulo Diego F. , Tapia Muñoz José Luis , Ramírez Morillo Ivon M. , Duno de Stefano Rodrigo 
Published: May 2023

Background: Centrosema (Fabaceae) includes 35-44 species in the New World, 10 in Mexico, and 9 in the Yucatan Peninsula. Most species have trifoliolate leaves and bicolored flowers. Unifoliolate leaves are an infrequent character that is found in a few species, e.g., C. sagittatum (Humb. & Bonpl. ex Willd.) Brandegee, but monochromatic flowers, e.g, lacking nectar guides, is even a more unusual character state. Several populations of a Centrosema from the Yucatan Peninsula share both character states, a combination that sets them apart from other members of the genus.
Question: Is the Centrosema with unifoliolate leaves, alate petioles, and monochromatic flowers of the Yucatan Peninsula, a species distinct from the phenetically similar C. sagittatum?
Studies species: Centrosema (Fabaceae, Papilionoideae).
Study site and dates: Yucatan Peninsula, Mexico, 2021-2023.
Methods: Botanical specimens were collected and determined taxonomically through the use of relevant literature, keys, and the consulting of botanical collections. The conservation status of the new species was assessed using the IUCN methodology.
Results: The specimens collected do represent an undescribed species that differs from Centrosema sagittatum by its smaller, cream-yellow, monochromatic flowers. The species is assessed as Endangered (EN).
Conclusions: A new species, Centrosema flavescens from the Yucatan Peninsula is proposed. Eleven species of Centrosema are now recognized in Mexico, nine in the Yucatan Peninsula. C. sagittatum does not occur in the Yucatan peninsula.