bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of toxicity and non-motor symptoms of Parkinson-like induced by rotenone in zebrafish animal model
Authors: de Andrade André Lucas Correa , Silva Renatta Priscilla Ferreira , Soares Priscila Rafaela Leão , Santos Thamiris Pinheiro , Padilha Renata Meireles Oliveira , Bastos Paulo Eduardo da Silva , Cadena Pabyton Gonçalves 
Published: January 2023

Abstract
In this study, the effects of exposure to rotenone on development were evaluated, particularly teratogenic and behavioral endpoints in the early life stages of zebrafish. This can serve as a model for Parkinson-like motor and non-motor symptoms, and anxiety-like behavior. The endpoints of percent epiboly, teratogenic effects, mortality, morphometry, thigmotaxis (TH), touch sensitivity (TS), and optomotor response (OMR) were analyzed in zebrafish embryo-larvae stage exposed to rotenone (5 to 20 µg/L). An increase in mortality of zebrafish was observed at 15 and 20 µg/L rotenone concentrations. The rotenone reduced the percent epiboly and increased the presence of teratogenic effects at concentrations of 10, 15, and 20 µg/L. Head and body size reductions were observed at all rotenone concentrations tested. Anxiety-like behaviors were observed with decreased displays of TH behavior in larvae exposed to 15 and 20 µg/L of rotenone. TS was reduced by 20 µg/L rotenone treatment. OMR and the eye diameter of zebrafish were not affected by rotenone exposure. Our results showed that rotenone has the potential to provoke non-motor symptoms, mainly anxiety-like behaviors, in the zebrafish's early life stages, making it a potential model for the study of Parkinson-like disease.
Toxicokinetics of recombinant human fibroblast growth factor 21 for injection in cynomolgus monkey for three months
Authors: Lu Chao , Jin Lei , Bi Jianing , Jin Hongyi , You Xinyi , Peng Lulu , Wang Liangshun , Fan Zhengkai , Wang Xiaojie 
Published: January 2023

Abstract
Recombinant human fibroblast growth factor 21 (FGF-21) is a potential therapeutic agent for multiple metabolic diseases. However, little is known about the toxicokinetic characteristics of FGF-21. In the present study, we investigated the toxicokinetics of FGF-21 delivered via subcutaneous injection in vivo. Twenty cynomolgus monkeys were injected subcutaneously with different doses of FGF-21 for 86 days. Serum samples were collected at eight different time points (0, 0.5, 1.5, 3, 5, 8, 12, and 24 h) on day 1 (d1), d37, and d86 for toxicokinetic analysis. The serum concentrations of FGF-21 were measured using a double sandwich ELISA. Necropsy and pathological analysis were performed on d87 and d116 (after recovery for 29 days). The average AUC(0-24h) values of low-dose FGF-21 on d1, d37, and d86 were 5253, 25268, and 60445 μg·h/L, and the average AUC(0-24h) values of high-dose FGF-21 on d1, d37, and d86 were 19964, 78999, and 1952821 μg·h/L, respectively. The peak concentrations (Cmax) of low-dose FGF-21 on d1, d37, and d86 were 621.1, 2767.9, and 4182.0 μg/L, respectively. The Cmax values of high-dose FGF-21 on d1, d37, and d86 were 2196.8, 6637.5, and 13430.4 μg/L, respectively. The elimination half-lives (t1/2z) of low- and high-dose FGF-21 on d1, d37, and d86 were 3.1–4.8, 4.2–6.7, and 6.2–8.8 h, respectively. The anatomical and pathological results showed that continuous subcutaneous injection of FGF-21 for 86 days did not affect organ weight, the organ coefficient, and histopathology in cynomolgus monkeys. Our results have guiding significance for the preclinical research and clinical use of FGF-21.
Functional Connectome Hierarchy in Schizotypy and Its associations with Expression of Schizophrenia-Related Genes
Authors: Dong Debo , Wang Yulin , Zhou Feng , Chang Xuebin , Qiu Jiang , Feng Tingyong , He Qinghua , Lei Xu , Chen Hong 
Published: January 2023

Abstract
Schizotype has been conceptualized as a continuum with symptoms of schizophrenia with marked genetic, neurobiological, sensory-cognitive overlaps. Hierarchical organization represents a general organizing principle for both the brain connectome supporting sensation-to-cognition continuum and gene expression patterns. However, the underlying changes in neuroimaging maps reflecting the cortical hierarchy that mechanistically link gene expressions to schizotypy are unclear. Using a large cohort of resting state-fMRI data from 1013 healthy young adults, the present study investigated schizotypy-associated sensorimotor-to-transmodal connectome hierarchy and assessed the connectome hierarchy similarity between schizotypy and schizophrenia. Furthermore, the normative and differential postmortem gene expression data were employed to investigate the transcriptional profiles associated with the schizotypy-associated connectome hierarchy. We found that schizotypy was associated with a compressed functional connectome hierarchy, including compressed global topography and focal alterations in sensory and transmodal cognitive areas, suggesting diminished functional system differentiation. Interestingly, the pattern of schizotypy-related hierarchy is tightly correlated with the pattern of hierarchy organization observed in schizophrenia. Notably, schizotypy-related connectome hierarchy was most closely colocated with expression of schizophrenia-related genes compared with other psychiatric disorders, with the correlated genes being enriched in biological processes well-known involved in schizophrenia, i.e., transsynaptic and receptor signaling, calcium ion binding, and channel activity. These findings not only shed light on the neurobiological and molecular genetic mechanisms underlying the sensory-cognitive deficits in schizotypy, but also provide new insights into the neurobiological continuum of psychosis thus advanced our understanding of how genetic propensity for schizophrenia-alike traits play an enduring role in creating biological vulnerability to psychosis.
Resveratrol induces proliferation and differentiation of mouse preosteoblast MC3T3-E1 by promoting autophagy
Authors: Cai Weiye , Sun Bin , Song Chao , Liu Fei , Wu Zhengliang , Liu Zongchao 
Published: January 2023

Abstract
Background: The MC3T3-E1 mouse pre-osteoblast can grow and differentiate more rapidly when resveratrol is present, according to study. Uncertainty exists over whether resveratrol (The following grouping is represented as Res) regulates the impact of autophagy on the proliferation and differentiation of mouse pre-osteoblast, despite the fact that autophagy is crucial in the control of osteoblast development.
Purpose: To investigate the involvement of autophagy in the proliferation and differentiation of mice pre-osteoblasts as a result of resveratrol.
Methods: 1） MC3T3-E1 cells were separated into blank control group and various concentrations (0.01, 0.1, 1, 10, 100μmol/L) of group in order to determine the ideal resveratrol concentration. In the resveratrol group, CCK8 was used to measure the proliferation activity of pre-osteoblasts in mice in each group after resveratrol intervention; ALP and alizarin red staining were used to gauge the degree of osteogenic differentiation; and RT-qPCR was used to measure the expression levels of Runx2 and OCN in the osteogenic differentiation ability of the cells. 2）In the experiment, four groups were set up: the control group, 3MA, Res group, and Res +3MA group. To examine cell mineralization, ALP and alizarin red staining were utilized. RT-qPCR and Western blot detection of cell autophagy activity levels and osteogenic differentiation capacity in each group following intervention.
Results: 1) Resveratrol might increase the number of mice pre-osteoblast, with the impact being most pronounced at 10μmol/L (P < 0.05); The nodules developed substantially more often than in the blank control group, and Runx2 and OCN expressions significantly increased (P < 0.05); 2) In contrast to the resveratrol group, after 3MA purine blocked autophagy, the Res+3MA group's alkaline phosphatase staining and the development of mineralized nodules were reduced; Runx2, OCN, LC3II / LC3I expression decreased, p62 expression increased (P <0.05).
Conclusion: By increasing autophagy, resveratrol may induce osteogenic differentiation of MC3T3-E1 cells.
Clinical efficacy of robot-assisted subxiphoid versus lateral thoracic approach in the treatment of anterior mediastinal tumors
Authors: Hong Ziqiang , Sheng Yannan , Bai Xiangdou , Cui Baiqiang , Lu Yingjie , Wu Xusheng , Cheng Tao , Jin Dacheng , Gou Yunjiu 
Published: January 2023

Abstract
Background: The purpose of this study was to compare the perioperative efficacy and safety of da Vinci robot assisted thoracoscopic surgery (RATS) for the treatment of anterior mediastinal tumors through the subxiphoid and lateral thoracic approaches under the anesthesia of non tracheal intubation (i.e. laryngeal mask airway).
Methods: We retrospectively analyzed the clinical data of 116 patients with anterior mediastinal tumors treated by RATS under laryngeal mask anesthesia completed by the same operator in the Department of Thoracic Surgery, Gansu Provincial People's Hospital from October 2016 to October 2022. There were 52 patients, 24 males and 28 females, with a mean age of (45.40±4.94) years, in the subxiphoid approach (subxiphoid group) and 64 patients, 34 males and 30 females, with a mean age of (46.86±5.46) years, in the lateral thoracic approach (lateral thoracic group). The operating time, intraoperative bleeding and total postoperative drainage in the two groups were compared and analyzed.
Results: All patients in both groups successfully completed resection of the anterior mediastinal tumor without the occurrence of perioperative death. In terms of total postoperative drainage flow [(229.90±25.00) ml vs. (242.03±35.99) ml], postoperative drainage time [(2.56±0.78) days vs. (2.97±0.94) days], postoperative hospital stay [(3.69±0.92) days vs. (4.14±1.13) days], and VAS pain on postoperative day 2 and 3,
the subxiphoid group had more advantages and the difference was statistically significant (P<0.05). In terms of docking time [(10.23±1.29) min vs. (12.40±2.13) min], the lateral chest group was more advantageous and the difference was statistically significant (P<0.05). There was no statistically significant difference between the two groups in terms of operative time, total operative time, total postoperative drainage, postoperative day 1 VAS pain score and postoperative complications (P>0.05).
Conclusion: The subxiphoid approach of RATS under laryngeal mask anesthesia is safe and feasible for resection of anterior mediastinal tumors. Compared with the lateral thoracic approach, the subxiphoid approach has advantages in terms of rapid postoperative recovery and postoperative patient pain, and patient acceptance is also higher, which is worth promoting in hospitals where it is available.
Factors influencing COVID-19 vaccine uptake among Nigerians: evidence from a national survey
Authors: OJO Temitope Olumuyiwa , OJO Ayodeji Oluwole , OJO Olajumoke Elizabeth , AKINWALERE Bosede Olufunmilayo , AKINWUMI Adebowale Femi 
Published: January 2023

Abstract
Objectives
. This study assessed the factors influencing COVID-19 vaccine uptake among Nigerian households. Data from the COVID-19 High-Frequency Phone Survey of Households that was collected in 2021 by the National Bureau of Statistics was used for this study. Relevant data was analyzed using descriptive statistical tools and the multivariate binary logistic regression model.
Results
Only 32 percent of the respondents were vaccinated against COVID-19. Respondents living in urban areas (34.4%) had a higher level of COVID-19 vaccine uptake relative to those living in rural Nigeria (30.9%). Results from the Multivariate Regression model revealed a significant association between COVID-19 vaccination uptake and age, access to health insurance, and information from health workers, government, and the media. The study also found households living in North Central, North East and South West had higher odds of being vaccinated. Therefore, the study recommended increased media campaigns and advocacy for COVID-19 vaccination. Younger persons should be targeted with COVID-19 information given that those aged 18–29 years were less likely to be vaccinated
Sorption properties of paper treated with silane-modified starch
Authors: Majka Jerzy , Perdoch Waldemar , Czajkowski Łukasz , Mazela Bartłomiej , Olek Wiesław 
Published: January 2023

Abstract
Sorption properties of paper products are important when applying paper as the packaging material. Hydrophobicity, i.e. reduced affinity of paper surface to liquid water, is of a particular importance from the point of view of the direct impact on packed goods. However, hygroscopicity of the material is significant during storage of goods. The analysis of changes in the hygroscopic properties of paper products induced by the treatment with silane modified starch was presented. The results on the hygroscopicity of treated paper were related to the previously published results on the hydrophobicity. The discrete values equilibrium moisture content allowed constructing adsorption and desorption isotherms. The three-parameter Guggenheim, Anderson, and De Boer (GAB) model, the four-parameter Generalized D’Arcy and Watt (GDW), and Yanniotis and Blahovec (Y-B) sorption isotherm models were used to quantify the hygroscopic properties for all options of untreated and modified paper samples. The effectiveness of silanes for improving hygroscopic properties was determined and compared to their impact on hydrophobicity of the studied materials. It was found that 1H,1H,2H,2H-perfluorooctyltriethoxysilane was the only silane improving hydrophobic and hygroscopic properties. The analysis of the applied models of sorption isotherms depicted that the GAB model was not valid for describing water sorption isotherms in the case of the tested materials, while the GDW and Y-B models provided a much more realistic description of water sorption mechanisms. The evaluation of the results of fitting the GDW model indicated that water molecules bonding to the primary sites was the dominating mechanism of sorption.
On doubly symmetric periodic orbits
Authors: Frauenfelder Urs , Moreno Agustin 
Published: January 2023

Abstract
In this article, for Hamiltonian systems with two degrees of freedom, we study doubly symmetric periodic orbits, i.e. those which are symmetric with respect to two (distinct) commuting antisymplectic involutions. These are ubiquitous in several problems of interest in mechanics. We show that, in dimension four, doubly symmetric periodic orbits cannot be negative hyperbolic. This has a number of consequences: (1) all covers of doubly symmetric orbits are good, in the sense of Symplectic Field Theory [6]; (2) a non-degenerate doubly symmetric orbit is stable if and only if its CZ-index is odd; (3) a doubly symmetric orbit does not undergo period doubling bifurcation; and (4) there is always a stable orbit in any collection of doubly symmetric periodic orbits with negative SFT-Euler characteristic (as coined in [11]). The above results follow from: (5) a symmetric orbit is negative hyperbolic if and only its two B-signs (introduced in [10]) differ.
Approximate blocked Gibbs sampling for Bayesian neural networks
Authors: Papamarkou Theodore 
Published: January 2023

Abstract
In this work, minibatch MCMC sampling
for feedforward neural networks is made more feasible.
To this end,
it is proposed to sample subgroups of parameters
via a blocked Gibbs sampling scheme.
By partitioning the parameter space,
sampling is possible irrespective of layer width.
It is also possible to alleviate vanishing acceptance rates
for increasing depth
by reducing the proposal variance in deeper layers.
Increasing the length of a non-convergent chain
increases the predictive accuracy in classification tasks,
so avoiding vanishing acceptance rates and
consequently enabling longer chain runs
have practical benefits.
Moreover, non-convergent chain realizations
aid in the quantification of predictive uncertainty.
An open problem is how to perform minibatch MCMC sampling
for feedforward neural networks
in the presence of augmented data.
Evidence-Based Policy Making: Analysis and Policy Recommendations for how the Philippines can manage a Cost-Effective Clean Energy Transition
Authors: Alexander Kane 
Published: January 2023

Abstract
The purpose of this research was to assess different scenarios for a cost-effective clean energy transition in the Philippines. This was done by using the Open Source Energy Modelling System (OSeMOSYS), which is a modelling software that can be used to project future energy capacities for a country, among other relevant factors such as capital investment and carbon emissions. The six scenarios modelled were least cost (LC), coal phase out (CPO), Philippines energy plan (PEP), combined energy plan (CEP), nationally determined contribution (NDC) and net zero (NZ). The key findings indicated that the CEP scenario was the most cost-effective scenario as the emissions are over 50% less compared to the LC scenario, as well as phasing out coal by 2050 and supporting the goals of the Philippines Development Plan. However, the ambitious NDC and NZ scenarios should be worked towards if funding can be secured to support them. For the Philippines, the main focus must be on the phasing out of coal, as well as a particular focus on certain renewables and the importance of finance, among other factors. This study therefore presents a discussion based on the results about the most crucial factors and what can be done to make an energy transition successful.
Creation of Fragrant Rice by Targeted Editing of fgr Gene Using Magnetic Nanoparticle- mediated Pollen Magnetofection in Rice
Authors: Shen Renjia , Peng Ziai , Zhao Lin , Chen Chun , Wang Hui , Chen Zhiqiang , Wang Jiafeng , Guo Tao 
Published: January 2023

Abstract
Background
CRISPR/Cas9 technology has been widely used for plant genome editing, while the delivery of gene editing components mainly depends on Agrobacterium or bombardment. They both need a long period of tissue culture process and some varieties are very difficult to regenerate from tissue culture, which seriously limits the process of gene editing.
Result
The CRISPR/Cas9 expression vector of fragrance gene fgr was constructed and combined with magnetic nano particles (MNPs) to form a gene carrier complex. To determine the suitable method of magnetofection, the appropriate mass ratios of MNPs / vector was explored, and the location of the complex in pollen was confirmed. The T1 plants obtained from the pollination of magnetofected pollen on wild-type rice showed decreased height and seed setting rate, with the hygromycin resistant rate (i.e. positive rate) 13.07% and 3 lined mutated. T2 plants were obtained from the self-crossing of positive T1 lines, with positive rate 58.80% and 8 lines mutated. According to the mutation of T1 and T2 generation, the genetic modes of magnetofected rice mainly included “continuous-generation editing” and “interval-generation editing”. It showed great potential that negative and homozygous mutated plants (ho/-C) could be obtained as early as in T2 generation in lines with “continuous-generation editing”.
Conclusion
Gene targeted editing mediated by pollen magnetofection is tissue culture independent, which shortens the breeding cycle, provides new technical support for rice breeding, and is of great significance to accelerate the breeding process of fine varieties.
Long non-coding RNA-GDA-1 Regulates Keratinocyte Proliferation and Psoriasis Inflammation by Regulating Forkhead Box M1 via the STAT3/NF-κB Signaling Pathway
Authors: li xueqing , chen fuqiang , Ju Jiaoying , Yin Xiran , Yang Zhenxian , Li Zhengjun , Sun Qing 
Published: January 2023

Abstract
Psoriasis is a chronic inflammatory skin disease associated with multiple comorbidities and complex pathogenesis. Long noncoding RNAs (lncRNAs) play an important regulatory role in many diseases, including psoriasis. In this study, We aimed to investigate the role and mechanism of lncRNA GDA-1 (GDA) in M5-treated psoriatic keratinocytes. GDA expression was significantly upregulated in psoriatic tissues and M5-treated keratinocytes. By silencing and overexpressing GDA in NHEKs and Ker-CT cells, we showed that GDA regulated proliferation and cell cycle, and increased secretion of interleukin-1β [IL‐1β], IL-6, chemokine ligands 2 and 20 (CCL2 and CCL20). RNA sequencing after GDA silencing led to identification of a close regulatory relationship between GDA and Forkhead Box M1 (FOXM1). GDA significantly influenced FOXM1 expression at both mRNA and protein levels and activated STAT3/NF-κB signaling pathways. STAT3 and NF-κB inhibition abrogated GDA effects on keratinocyte proliferation and inflammation. In conclusion, our study is the first to report that Lnc-GDA-1 distinctly regulates FOXM1 expression and mediates proliferation and inflammation of psoriatic keratinocytes through the STAT3/NF-κB signaling pathway, which may be a potent target for psoriasis treatment.
Superresolution fluorescence microscopy of platelet subcellular structures as a potential tumor liquid biopsy
Authors: Zhang Yu-Hui , Xu Peng , Deng Huan , Hong Zhenya , Zhong Simei , Chen Feifan , Wang Liangliang , Wang Zhenhao , Mei Yu , Luo Ziying , He Ziliang , Li Haiwen , Gan Chao , Zhang Huaqiu , Ma Yan , Han Zhiqiang 
Published: January 2023

Abstract
Blood-based tumor liquid biopsies are promising as an alternative or complement to tissue biopsies due to their noninvasiveness, convenience, and safety, and there is still a great demand for the discovery of new biomarkers for these biopsies. Here, we present nanoscale distribution patterns of subcellular structures in platelets, as imaged by structured illumination superresolution fluorescence microscopy, as a new type of potential biomarker for tumor liquid biopsies. We established a standardized protocol for platelet sample preparation and developed an automated high-throughput image analysis workflow. We explored the diagnostic capability based on the statistical analysis of 200,000 superresolution images of individual platelets from a variety of tumor patients, benign mass patients, and healthy volunteers (n=163). Our results suggest that the nanoscale distribution patterns of α-granules in platelets have the potential to be biomarkers for several cancers, including glioma and cervical, endometrial and ovarian cancers, facilitating not only diagnosis but also therapeutic monitoring. This study provides a promising novel type of platelet parameter for tumor liquid biopsies at the subcellular level rather than the existing cellular or molecular level and opens up a new avenue for clinical applications of superresolution imaging techniques.