bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insight into the Substrate Specificity of Lactobacillus paracasei Aspartate Ammonia-Lyase
Authors: Huang Yi-Hao , You Weir-Chiang , Chen Yung-Ju , Ciou Jhih-Ying , Hsieh Lu-Sheng 
Published: January 2023

Aspartate ammonia-lyase (AAL) catalyzes the reversible conversion reactions of aspartate to fumaric acid and ammonia. In this work, Lactobacillus paracasei LpAAL gene was heterologously expressed in Escherichia coli. As well as a recombinant His-tagged LpAAL protein, a maltose-binding protein (MBP) fused LpAAL protein was used to enhance its protein solubility and expression level. Both recombinant proteins showed broad substrate specificity, catalyzing aspartic acid, fumaric acid, phenylalanine, and tyrosine to produce fumaric acid, aspartic acid, trans-cinnamic acid, and p-coumaric acid, respectively. The optimum reaction pH and temperature of LpAAL protein for four substrates were measured at 8.0 and 40 °C, respectively. The Km values of LpAAL protein for aspartic acid, fumaric acid, phenylalanine, and tyrosine as substrates were 5.7, 8.5, 4.4, and 1.2 mM, respectively. The kcat values of LpAAL protein for aspartic acid, fumaric acid, phenylalanine, and tyrosine as substrates were 6.7, 0.45, 4.96, and 0.02 s−1, respectively. Therefore, aspartic acid, fumaric acid, phenylalanine, and tyrosine are bona fide substrates for LpAAL enzyme.
CDT of Clostridioides difficile Induces MLC-Dependent Intestinal Barrier Dysfunction in HT-29/B6 Epithelial Cell Monolayers
Authors: Heils Lucas , Schneemann Martina , Gerhard Ralf , Schulzke Jörg-Dieter , Bücker Roland 
Published: January 2023

Background: Clostridioides difficile binary toxin (CDT) defines the hypervirulence of strains in nosocomial antibiotic-induced colitis with the highest mortality. The objective of our study was to investigate the impact of CDT on the intestinal epithelial barrier and to enlighten the underlying molecular mechanisms. Methods: Functional measurements of epithelial barrier function by macromolecular permeability and electrophysiology were performed in human intestinal HT-29/B6 cell monolayers. Molecular analysis of the spatial distribution of tight junction protein and cytoskeleton was performed by super-resolution STED microscopy. Results: Sublethal concentrations of CDT-induced barrier dysfunction with decreased TER and increased permeability for 332 Da fluorescein and 4 kDa FITC-dextran. The molecular correlate to the functional barrier defect by CDT was found to be a tight junction protein subcellular redistribution with tricellulin, occludin, and claudin-4 off the tight junction domain. This redistribution was shown to be MLCK-dependent. Conclusions: CDT compromised epithelial barrier function in a human intestinal colonic cell model, even in sublethal concentrations, pointing to barrier dysfunction in the intestine and leak flux induction as a diarrheal mechanism. However, this cannot be attributed to the appearance of apoptosis and necrosis, but rather to an opening of the paracellular leak pathway as the result of epithelial tight junction alterations.
Uso de Levosimendán en Insuficiencia Cardíaca Aguda e Insuficiencia Cardíaca Crónica Avanzada: revisión de evidencia
Authors: González Quesada Andrea Pamela , Badilla Casasola Alexa , Arroyo Quirós María Alejandra , Gutiérrez Castro Douglas Manuel 
Published: January 2023

El levosimendán es un inodilatador que se caracteriza por su inotropía positiva, vasodilatación y cardioprotección; mecanismo de acción único que lo diferencia de todos los demás fármacos inotrópicos. Además, cuenta con el metabolito activo, OR-1896, que le permite mantener sus efectos terapéuticos por varios días, aun después de finalizada la infusión.
Actualmente se han demostrado sus efectos potenciales para manejar la insuficiencia cardíaca aguda y la insuficiencia cardíaca crónica avanzada, aportando una nueva opción para los pacientes cuando la terapia convencional no está siendo efectiva en el control de la enfermedad.
Total spectral power-based method for estimating Brillouin frequency shift in optical fibers
Authors: Sasage Haruki , Sakamoto Mana , Noda Kohei , Nakamura Kentaro , Mizuno Yosuke , Lee Heeyoung 
Published: January 2023

Abstract
A cost-effective method for estimating the Brillouin frequency shift (BFS) of the whole sensing fiber without using an electrical spectrum analyzer is developed. The Brillouin gain spectrum is down-converted and low-pass-filtered, and its total transmitted power is used to estimate the BFS. The strain dynamic range of this method is experimentally shown to be double the value of a conventional slope-assisted method.
Proactive Edge Caching in Vehicular Networks: An Online Bandit Learning Approach
Authors: Wang Qiao , Grace David 
Published: January 2023

<p>Proactively caching content at the network edge is particularly effective in high-mobility vehicular networks, where intermittent connection is the major challenge for seamless content transmission. The objective of this paper is to achieve proactive caching in vehicular networks by mobility prediction, specifically by predicting the next roadside unit (RSU) for a vehicle with reinforcement learning techniques. The paper proposes two proactive caching algorithms based on multi-armed bandit (MAB) learning, non-contextual MAB and contextual MAB, respectively. This paper fills the void in the literature regarding the application of MAB learning to mobility-prediction based proactive caching. Their feasibility, superiority, and applicability are evaluated with simulation in two modern cities: Las Vegas, USA with a grid road layout, and Manchester, UK with a more historical layout. Additionally, this paper is the first that proposes to investigate the uncertainty associated with proactive caching systems in the form of entropy with a specifically extended Subjective Logic framework, in order to provide an insight into the underlying link between prediction accuracy and uncertainty. </p>
On the hit problem for the polynomial algebra and the algebraic transfer
Authors: Võ Phúc Đặng 
Published: January 2023

<p>Let $\mathcal A$ be the classical, singly-graded Steenrod algebra over the prime order field $\mathbb F_2$ and let $P^{\otimes h}: = \mathbb F_2[t_1, \ldots, t_h]$ denote the polynomial algebra on $h$ generators, each of degree $1.$ Write $GL_h$ for the usual general linear group of rank $h$ over $\mathbb F_2.$ Then, $P^{\otimes h}$ is an $\mathcal A[GL_h]$-module. As well known, the (mod 2) cohomology groups of the Steenrod algebra, ${\rm Ext}_{\mathcal A}^{h, h+*}(\mathbb F_2, \mathbb F_2)$ is still largely mysterious for all homological degrees $h \geq 6.$ The $h$-th algebraic transfer $Tr_h^{\mathcal A}: (\mathbb F_2\otimes_{GL_h}{\rm Ann}_{\overline{\mathcal A}}[P^{\otimes h}]^{*})_n\longrightarrow {\rm Ext}_{\mathcal A}^{h, h+n}(\mathbb F_2, \mathbb F_2),$ defined by Singer [Math. Z. \textbf{202} (1989), 493-523], is a helpful tool to describe that Ext groups. Singer conjectured that this homomorphism is a monomorphism for any $h>0.$ There is currently no information about the conjecture for $h = 6.$ The purpose of this Note is to study Singer's conjecture for all $h\geq 1$ in degrees $n\leq 10 = 6(2^{0}-1) + 10\cdot 2^{0}$ and for $h=6$ in general degree $n:=n_s=6(2^{s}-1) + 10\cdot 2^{s},\, s\geq 0.$ Our approach is based on techniques of the hit problem for $P^{\otimes h}.$ This also extends a result in Mothebe et al. [J. Math. Res. \textbf{8} (2016), 92-100] on the hit problem for $P^{\otimes 6}$ in degree $n_s.$ More generally, for $h\geq 6,$ we show that the dimension of the cohit module $\mathbb F_2\otimes_{\mathcal A}P^{\otimes h}$ in degrees $2^{s+4}-h$ is equal to the order of the factor group of $GL_{h-1}$ by the Borel subgroup $B_{h-1}$ for every $s\geq h-5.$ Especially, for the Galois field $\mathbb F_{q}$ ($q$ denoting the power of a prime number), based on Hai's recent work [C. R. Math. Acad. Sci. Paris \textbf{360} (2022), 1009-1026], we claim that the dimension of the space of the indecomposable elements of $\mathbb F_q[t_1, \ldots t_h]$ in degrees $q^{h-1}-h$ is equal to the order of the factor group of $GL_{h-1}(\mathbb F_q)$ by a subgroup of the Borel group $B_{h-1}(\mathbb F_q).$ As applications, we establish the dimension result for the cohit module $\mathbb F_2\otimes_{\mathcal A}P^{\otimes 7}$ in degrees $n_{s+5},\, s > 0.$ At the same time, we prove that the non-zero elements $h_2^{2}g_1 = h_4Ph_2\in {\rm Ext}_{\mathcal A}^{6, 6+n_1}(\mathbb F_2, \mathbb F_2)$, and $D_2\in {\rm Ext}_{\mathcal A}^{6, 6+n_2}(\mathbb F_2, \mathbb F_2)$ are not in the image of $Tr_6^{\mathcal A}.$ We also give some conjectures on the $h$-th cohomology groups of the algebra $\mathcal A$ for $h\geq 6,$ and discuss their relationship to previous works. </p>
Rhodes: A Next-Generation OS based on Resource Governance Model
Authors: Wang Changsheng 
Published: January 2023

<p>The kernel is the core part of an operating system. At present, four types of kernels are widely available: monolithic kernels, microkernels, hybrid kernels, and exokernels. These kernel architectures are either too tightly-coupled, like monolithic kernels, put too many modules into the kernel, or too free, like exokernels, allow users to freely develop core features such as IPC, Virtual Memory, etc, or have no rules, like hybrid kernels, kernel modules organization is uncontrolled. In recent years, to solve these problems, resource management concept frequently appears in industry and academia. Although resource management concept makes resources in OSes orderly, no existing OSes or software systems can modify and extend management rules conveniently and dynamically.</p>
<p>This paper proposes a resource governance model that places resources such as applications and hardware into the model. Through unified rule governance, the organization of resources is more orderly and scalable. At the same time, by separating rules from resources, rules in the model are also scalable and flexible. Based on the model, we built Rhodes OS that allows users to modify and extend resource management rules by governance center which separates rules from resources. In addition, Rhodes also fits all kinds of OSes that are optimized for specific scenarios such as mobile, big data, IoT and AI. We implemented Rhodes on x86-64 and evaluated it. Our evaluation results show that applications performance in Rhodes is equivalent to Linux in native mode. So we believe that OSes will enter resource governance age from resource management age in the near future.</p>
Finite Set Sensorless Control With Minimum a Priori Knowledge and Tuning Effort for Interior Permanent Magnet Synchronous Motors
Authors: Brosch Anian , Tinazzi Fabio , Wallscheid Oliver , Zigliotto Mauro , Böcker Joachim 
Published: January 2023

<p>By applying rotor angle sensorless control methods the costs of the electrical drive can be decreased while its reliability is increased. Traditionally, the design of both the rotor angle estimator and the drive controller require a detailed motor model and manual tuning leading to significant effort by human experts. In this work, a rotor anisotropy-based sensorless control scheme for interior permanent magnet synchronous motors (IPMSM) is proposed that enables sensorless control with minimum tuning effort and a priori motor knowledge. Due to these characteristics the scheme is particularly suitable for self-commissioning or low-cost drive applications. The scheme contains a finite control set model predictive current controller (FCS-MPCC) with an additional inequality constraint that enables the identification of the motor model in the stator-fixed coordinate system by using the last three measurement samples. Utilizing an automatic system identification procedure, the motor model is determined online in a data-driven fashion. The identified motor model serves both as prediction model of the FCS-MPCC and as baseline for the rotor angle estimation via an eigenvalue decomposition approach. Challenging experimental investigations at standstill up to the medium speed range prove the applicability of the proposed approach. Here, highly dynamic speed and current transients can be handled by the proposed method.</p>
<p><br></p>
On the equation used to calculate lens surface radius of curvature based on the number of Newton’s ring
Authors: Sun Haiyin 
Published: January 2023

<p>Newton’s ring is the most used method to test surface radius of curvature of a lens. An approximate equation is currently widely used by lens fabrication shops and in literatures to calculate the error of surface radius of curvature based on the number of exhibited Newton’s ring. It is shown in this paper that this approximate equation contains significant error and should be replaced by two other simple equations derived in a recently published paper. </p>
Master Data Management using Machine Learning Techniques: MDM Bot
Authors: Behera Tapan , Panda BS 
Published: January 2023

<p>In today’s world of loosely coupled and distributed applications, communication and data centralization play a vital role. In large organizations, there are many applications and many teams that work on the data of customers, products, suppliers, and other business entities. Each application and team maintain a separate copy of the data, which means any application requiring this information needs to contact several other systems to obtain the most recent data for that entity. Therefore, this would increase the number of multiple lookups on various systems, resulting in an increase in the throughput of the application. To avoid this, the organization needs a centralized system called ”Master Data Management” that will maintain all the golden data across all the systems within the organization. Any system that is updated with new information will also be sent to MDM through events, and MDM will then be responsible for communicating with and updating the sub-systems within the organization’s Eco-System. Event-Driven Architecture will be used to communicate between distributed applications to achieve and implement Master Data Management. In recent years, there have been a lot of issues discovered in the MDM System between the data sync-up of all the sub-systems, resulting in a tedious process of identifying manually all the failures of the events, reprocessing the event information, correcting the data,and re-synchronizing the other sub-systems with all the previous ones. To avoid this manual process and to address the issues mentioned above, we introduce the ”MDM Bot” in this article. Artificial Intelligence and Machine Learning capability are included in the proposed MDM Bot.It is an excellent value-added Bot for an Enterprise System that helps and saves a lot of time and money.</p>
Predictors of Oncologic Outcome in Patients Receiving Phase I Investigational Therapy for Recurrent or Metastatic Cervical Cancer
Authors: Son Ji , Lin Heather Y. , Fu Siqing , Biter Amadeo B. , Dumbrava Ecaterina E. , Karp Daniel D. , Naing Aung , Pant Shubham , Piha-Paul Sarina A. , Rodon Jordi , Subbiah Vivek , Tsimberidou Apostolia M. , Yap Timothy A. , Frumovitz Michael M. , Jazaeri Amir A. , Ramirez Pedro T. , Westin Shannon N. , Yuan Ying , Meric-Bernstam Funda , Hong David S. 
Published: January 2023

ABSTRACT

Introduction
We aimed to identify clinical, pathologic, and treatment factors that are predictive of response and survival in patients with cervical cancer referred to phase I clinical trials.

Methods
Patients with cervical cancer who received at least one dose of a phase I investigational agent at our institution between 2014 and 2022 were included. The log-rank test was used to analyze differences in progression-free survival (PFS) and overall survival (OS), and multivariable regression analysis was performed.

Results
We included 65 patients with a median age of 41 years (range, 20–74), 3 prior therapies (range, 1–7), and 67.7% squamous carcinoma. The rate of distant metastasis at trial entry was 84.6%. The most common molecular alterations included PIK3CA (46.5%), PD-L1+ (46.2%), EPH (30.0%), and CREBBP (23.1%); 23.1% had received a prior checkpoint inhibitor. Phase I trials were for immunotherapy (58.5%) or targeted therapy (41.5%). The rate of biomarker matching was 21.5%. For all patients, median PFS was 3.6 months (95% CI, 2.0–5.2) and OS was 9.3 months (95% CI, 7.0–10.6). Factors at study entry associated with worse survival were presence of bone metastasis (PFS 1.6 vs 4.4 months: hazard ratio [HR], 2.8; p = 0.001; OS 3.8 vs 10.0 months: HR, 3.9; p &lt; 0.0001) and absolute lymphocyte count below 1000/μL (PFS 1.8 vs 5.2 months: HR, 2.9; p = 0.0004; OS 7.0 vs 10.6 months: HR, 3.2; p = 0.0009). Factors associated only with worse OS were absolute neutrophil count above 4700/μL, hemoglobin below 10.5 g/dL, and smoking status. Grade 3+ treatment-related adverse events were seen in 16.9% of cases.

Conclusion
Bone metastasis and absolute lymphocyte count below normal range at phase I study entry portend poor survival in patients with recurrent or metastatic cervical cancer.
State of harmful entomocomplex in winter wheat crops in Ukraine under the conditions of climate change
Authors: Borzykh O. , Chaika V. , Fedorenko A. , Borusenko V. , Neverovska T. , Vlasenko I. , Miniailo N. 
Published: January 2023

Goal. To establish the multi-year dynamics of the number of the main pests of winter wheat and the indicator of entomological diversity of agricultural landscapes in the conditions of climate change in Ukraine.
Methods. Laboratory, statistical and analytical. The state of populations during 2005—2017 was studied using the example of an indicator group of species — the entomological complex of the main pests of winter wheat crops. As initial data, a long-term database of the number of populations of the entomocomplex in different natural and climatic zones, which is monitored by specialists of the phytosanitary safety department of the State Production and Consumer Service of Ukraine, was used for many years.
Results. According to the analysed data on the state of indicator populations during 2009—2017, the indicators of the population size and weighted LPI were constantly decreasing. The correlation between the size of an insect’s population and the course of natural warming does not make it possible to unambiguously explain the state of populations by the effect of an increased amount of heat.
Conclusions. 1. The analysis of the long-term state of populations of a harmful entomocomplex of winter wheat as an indicator group shows that pests react differently to warming depending on the biology of the species and allows us to come to a conclusion about the gradual decrease in the number of insects in agricultural landscapes under conditions of climate warming in Ukraine. This finding is in good agreement with the literature on global depletion of invertebrate populations. 2. Under the conditions when most species of the harmful entomocomplex of winter wheat in different natural and climatic zones of Ukraine show depression against the background of global warming, planned chemical treatments against pests should be carried out only after careful phytosanitary monitoring of agrocenoses in order to clarify the degree of threat to the crop, which will help reduce pesticide load on the natural environment.
The influence of fertilizers on the damage of spring barley plants by intrastalk pests and grain yield
Authors: Kuzmenko N. 
Published: January 2023

Goal. To study the population and harmfulness of intrastalk pests in the agrocenosis of spring barley depending on the use of fertilizers.
Methods. The investigations was carried out in a stationary field, fixed nine-course-fallow-grain-row crop rotation at the Department for Plant Production and Cultivar Investigations of the Plant Production Institute named after V.Ya. Yuriev of the National Academy of Sciences (Eastern Forest-Steppe of Ukraine) during 2011—20.
Results. During the years of research, the species composition of intrastalk pests was represented by: Oscinella pusilla Mg., O. frit L., Chaetocnema hortensis Geoffr., and Mayetiola destructor Say. Oscinella pusilla Mg. and O. frit L. dominated, damage to shoots by their larvae varied from 1.9% (2016) to 61.1% (2013). Damage to shoots by Chaetocnema hortensis Geoffr. larvae ranged from 0.4% (2015) to 35.5% (2011). The greatest damage to shoots by the larvae of Mayetiola destructor Say. was registered in 2018 — 5.7%. Application of fertilizers (manure 6.6 t/ha of the crop rotation area, aftereffect, and mineral fertilizers in the norm N30P30K30 or N45P45K45) provided increased resistance of spring barley plants to damage by pests. On the average over 2011…2020 year period, in the block without fertilizers, damage to shoots by Oscinella pusilla Mg. and O. frit L. larvae was 21.6%, in the fertilized block — 19.3% (decrease at the tendency level). At the tillering stage, the undamaged shoots on the background without fertilizers was 72.5%, on the fertilized background — 76.1% (difference at the tendency level). A reliable converse average correlation was established between the damage of shoots by intrastalk pests and the grain yield of spring barley (r = –0.5). Averaged over ten years, the grain yield in the block without fertilizers was 3.25 t/ha, with the application of fertilizers — 4.69 t/ha, there was a difference of 30.7%.
Conclusions. The positive role of fertilizers in regulating the number of intrastalk pests of spring barley and increasing grain yield was established.