bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Aging and a Dual Orexin Receptor Antagonist on Sleep Architecture and Non-REM Oscillations Including an REM Behavior Disorder Phenotype in the PS19 Mouse Model of Tauopathy
Authors: Kam Korey , Vetter Kenny , Tejiram Rachel A. , Pettibone Ward D. , Shim Kaitlyn , Audrain Mickael , Yu Liping , Daehn Ilse S. , Ehrlich Michelle E. , Varga Andrew W. 
Published: May 2023

The impact of tau pathology on sleep microarchitecture features, including slow oscillations, spindles, and their coupling, has been understudied, despite the proposed importance of these electrophysiological features toward learning and memory. Dual orexin receptor antagonists (DORAs) are known to promote sleep, but whether and how they affect sleep microarchitecture in the setting of tauopathy is unknown. In the PS19 mouse model of tauopathy MAPT (microtubule-associated protein tau) P301S (both male and female), young PS19 mice 2–3 months old show a sleep electrophysiology signature with markedly reduced spindle duration and power and elevated slow oscillation (SO) density compared with littermate controls, although there is no significant tau hyperphosphorylation, tangle formation, or neurodegeneration at this age. With aging, there is evidence for sleep disruption in PS19 mice, characterized by reduced REM duration, increased non-REM and REM fragmentation, and more frequent brief arousals at the macrolevel and reduced spindle density, SO density, and spindle-SO coupling at the microlevel. In ∼33% of aged PS19 mice, we unexpectedly observed abnormal goal-directed behaviors in REM, including mastication, paw grasp, and forelimb/hindlimb extension, seemingly consistent with REM behavior disorder (RBD). Oral administration of DORA-12 in aged PS19 mice increased non-REM and REM duration, albeit with shorter bout lengths, and increased spindle density, spindle duration, and SO density without change to spindle–SO coupling, power in either the SO or spindle bands, or the arousal index. We observed a significant effect of DORA-12 on objective measures of RBD, thereby encouraging future exploration of DORA effects on sleep-mediated cognition and RBD treatment.SIGNIFICANCE STATEMENTThe specific effect of tauopathy on sleep macroarchitecture and microarchitecture throughout aging remains unknown. Our key findings include the following: (1) the identification of a sleep EEG signature constituting an early biomarker of impending tauopathy; (2) sleep physiology deteriorates with aging that are also markers of off-line cognitive processing; (3) the novel observation that dream enactment behaviors reminiscent of RBD occur, likely the first such observation in a tauopathy model; and (4) a dual orexin receptor antagonist is capable of restoring several of the sleep macroarchitecture and microarchitecture abnormalities.
Presynaptic targeting of botulinum neurotoxin type A requires a tripartitePSG‐Syt1‐SV2plasma membrane nanocluster for synaptic vesicle entry
Authors: Joensuu Merja , Syed Parnayan , Saber Saber H , Lanoue Vanessa , Wallis Tristan P , Rae James , Blum Ailisa , Gormal Rachel S , Small Christopher , Sanders Shanley , Jiang Anmin , Mahrhold Stefan , Krez Nadja , Cousin Michael A , Cooper‐White Ruby , Cooper‐White Justin J , Collins Brett M , Parton Robert G , Balistreri Giuseppe , Rummel Andreas , Meunier Frédéric A 
Published: May 2023

AbstractThe unique nerve terminal targeting of botulinum neurotoxin type A (BoNT/A) is due to its capacity to bind two receptors on the neuronal plasma membrane: polysialoganglioside (PSG) and synaptic vesicle glycoprotein 2 (SV2). Whether and how PSGs and SV2 may coordinate other proteins for BoNT/A recruitment and internalization remains unknown. Here, we demonstrate that the targeted endocytosis of BoNT/A into synaptic vesicles (SVs) requires a tripartite surface nanocluster. Live‐cell super‐resolution imaging and electron microscopy of catalytically inactivated BoNT/A wildtype and receptor‐binding‐deficient mutants in cultured hippocampal neurons demonstrated that BoNT/A must bind coincidentally to a PSG and SV2 to target synaptic vesicles. We reveal that BoNT/A simultaneously interacts with a preassembled PSG‐synaptotagmin‐1 (Syt1) complex and SV2 on the neuronal plasma membrane, facilitating Syt1‐SV2 nanoclustering that controls endocytic sorting of the toxin into synaptic vesicles. Syt1 CRISPRi knockdown suppressed BoNT/A‐ and BoNT/E‐induced neurointoxication as quantified by SNAP‐25 cleavage, suggesting that this tripartite nanocluster may be a unifying entry point for selected botulinum neurotoxins that hijack this for synaptic vesicle targeting.
Insulinoma with equivocal imaging
Authors: Belabed Wafa , Mnif Fatma , Missaoui Abdel Mouhaymen , Elleuch Mouna , Ben Salah Dhoha , Charfi Nadia , Mnif Mouna , Rekik Nabila , Hadj Kacem Faten , Abid Mohamed 
Published: May 2023

Summary
A 55-year-old patient was admitted to our department for the management of a repetitive alteration of consciousness. Biological investigation results were consistent with endogenous hyperinsulinemic hypoglycemia. Insulinoma was therefore suspected. Abdominal computed tomography and endoscopic ultrasound showed no obvious pancreatic mass.Somatostatin receptor scintigraphy showed abnormal radioactive uptake in both the pancreatic tail and the uncinate process. Contrariwise, abdominal magnetic resonance imaging showed a unique lesion in the pancreas tail. The patient was then proposed for pancreatic surgery. Both intraoperative manual palpation and intraoperative ultrasonography of the pancreas showed a single corporal lesion of 1.5 cm. No lesion was found in the uncinate process. After a left pancreatectomy, the lesion was histopathologically confirmed to be a well-differentiated neuroendocrine tumor. The symptoms of the patient resolved almost immediately following the surgery. The follow-up is one and a half years to date.

Learning points

The exact preoperative localization of the pancreatic mass remains the most challenging part of insulinoma diagnostic workup.

The radiologist’s experience is the best warrantor to a precise localization of the tumor.

111In-DTPA-octreotide uptake in the pancreatic uncinate process may be physiological and its interpretation must, therefore, be vigilant.

Manual palpation along with intraoperative ultrasonography is considered as the most effective method for the localization of insulinomas during open surgery.
Diagnóstico de salud ocupacional en la empresa de curtiembres Cueros JCG
Authors: Lozano Rodríguez Carlos Gerardo , Rubio Cárdenas Carlos Andrés 
Published: May 2023

Las pieles son consideradas un subproducto de la industria de la carne y mataderos, en donde, el 59% de las empresas del sector de curtiembres en Colombia, en su gran mayoría son microempresas las cuales no cuentan con los procesos y tecnología necesaria para dar un buen trato al animal y posteriormente al cuero (López y Hueza, 2018). De acuerdo con la Guía de Producción Limpia para el Sector Curtiembres de Bogotá (2017), los procesos de transformación de los cueros se caracteriza por su excesivo consumo de agua; a su vez el uso desproporcionado de productos químicos por tonelada de piel procesada, siendo el proceso de curtido el más contaminante. Las condiciones actuales del proceso de producción en la empresa Cueros JCG provocan un impacto ambiental negativo que afecta a la comunidad cercana a la empresa, así como bajos niveles de productividad y un alto riesgo de sanciones por el incumplimiento de las normas ambientales. En ese sentido se propone desde la comprensión de las condiciones actuales de la empresa, el diseño de una propuesta de mejora del proceso de recurtido que considere la incorporación de tecnologías limpias, de fácil acceso y que a su vez mejore las condiciones higiénicas en los puestos de trabajo en la empresa. Los resultados obtenidos en el presente trabajo evidenciaron que, el 75% de los riesgos afectan a la salud y la vida, el 17.1% están relacionados con el contacto y/o inhalación con productos químicos como pinturas, disolventes, anticorrosivos y el 8.6% tienen que ver con condiciones no idóneas de orden y aseo.
Comunicación intercultural en contextos atravesados por la diversidad cultural
Authors: Perusset Macarena 
Published: May 2023

Este trabajo aborda las posibilidades de comunicación intercultural de distintos profesionales de la salud en una organización sanitaria de gestión privada en la provincia de Córdoba que presenta una población de pacientes diversa. El objetivo de investigación es identificar los distintos elementos que se encuentran en la comunicación efectiva con los pacientes. Al respecto, se considera que los estudios previos han prescindido de esta realidad, pese a ser de vital importancia en la comunicación entre pacientes y profesionales de la salud, al poder resultar en malentendidos, diagnósticos errados y generar insatisfacción en la atención. En este artículo se incluyen las percepciones de los propios pacientes sobre la capacidad de comunicación con los profesionales, para ello se aplicó la técnica de observación participante durante dieciocho semanas y se realizaron encuestas a médicos de distintas especialidades y enfermeros; asimismo, a pacientes ambulatorios e internados en la institución. En las encuestas realizadas se solicitó a los profesionales de la salud que calificaran su capacidad de comunicación con personas pertenecientes a otras culturas, mientras que en las encuestas realizadas a los pacientes, se les solicitó que valoraran la capacidad de comunicación de los profesionales de la salud . Se analizaron las respuestas de 22 profesionales (tanto médicos como enfermeros) y 91 pacientes. Los resultados demuestran que los profesionales que presentan mayor empatía y experiencia intercultural previa tienen mayores posibilidades de alcanzar una comunicación efectiva con los pacientes, con respecto al cuidado de la salud de estos últimos.
Las metáforas en las adivinanzas o watuchis quechuas: un estudio desde la semántica cognitiva
Authors: Palomino Niel , Arredondo Roxssana 
Published: May 2023

El propósito del estudio es identificar, clasificar y exponer las metáforas en las adivinanzas o watuchis quechuas, así como los procedimientos cognitivos para su creación. Para ello, se indagará en la teoría de las metáforas propuesta por Lakoff y Johnson (1980), en el libro Metaphors We Live By. Metodológicamente, el corpus se encuentra integrado por cincuenta adivinanzas que fueron recopiladas y transcritas del libro Watuchikuna. El cual ha sido publicado en 1986 por el Centro Andino de Educación y Promoción (CADEP) "José María Arguedas" (Cusco-Perú). Los resultados muestran que, en las adivinanzas quechuas, los objetos, los frutos y los animales son personas; las plantas, animales; los animales, objetos; los objetos, seres vivos. Asimismo, que el cuerpo es planta y el camino es objeto. El artículo concluye que la mayoría de los watuchis quechuas son ontológicas. Además, en la experiencia perceptual, corpórea y cultural del quechuahablante la tierra es un ser que tiene vida. Por lo tanto, todo lo que en ella existe tiene vida o se personifica para ser expresada mediante las adivinanzas.
Randomized Trial to Assess the Clinical Utility of Renal Allograft Monitoring by Urine CXCL10 Chemokine
Authors: Hirt-Minkowski Patricia , Handschin Joelle , Stampf Susanne , Hopfer Helmut , Menter Thomas , Senn Lisa , Hönger Gideon , Wehmeier Caroline , Amico Patrizia , Steiger Jürg , Koller Michael , Dickenmann Michael , Schaub Stefan 
Published: May 2023

Significance Statement
This study is the first randomized controlled trial to investigate the clinical utility of a noninvasive monitoring biomarker in renal transplantation. Although urine CXCL10 monitoring could not demonstrate a beneficial effect on 1-year outcomes, the study is a rich source for future design of trials aiming to explore the clinical utility of noninvasive biomarkers. In addition, the study supports the use of urine CXCL10 to assess the inflammatory status of the renal allograft.

Background
Urine CXCL10 is a promising noninvasive biomarker for detection of renal allograft rejection. The aim of this study was to investigate the clinical utility of renal allograft monitoring by urine CXCL10 in a randomized trial.

Methods
We stratified 241 patients, 120 into an intervention and 121 into a control arm. In both arms, urine CXCL10 levels were monitored at three specific time points (1, 3, and 6 months post-transplant). In the intervention arm, elevated values triggered performance of an allograft biopsy with therapeutic adaptations according to the result. In the control arm, urine CXCL10 was measured, but the results concealed. The primary outcome was a combined end point at 1-year post-transplant (death-censored graft loss, clinical rejection between month 1 and 1-year, acute rejection in 1-year surveillance biopsy, chronic active T-cell–mediated rejection in 1-year surveillance biopsy, development of de novo donor-specific HLA antibodies, or eGFR <25 ml/min).

Results
The incidence of the primary outcome was not different between the intervention and the control arm (51% versus 49%; relative risk (RR), 1.04 [95% confidence interval, 0.81 to 1.34]; P = 0.80). When including 175 of 241 (73%) patients in a per-protocol analysis, the incidence of the primary outcome was also not different (55% versus 49%; RR, 1.11 [95% confidence interval, 0.84 to 1.47]; P = 0.54). The incidence of the individual end points was not different as well.

Conclusions
This study could not demonstrate a beneficial effect of urine CXCL10 monitoring on 1-year outcomes (ClinicalTrials.gov_NCT03140514).
The Community Stress-Drop Validation Study—Part II: Uncertainties of the Source Parameters and Stress Drop Analysis
Authors: Bindi Dino , Spallarossa Daniele , Picozzi Matteo , Oth Adrien , Morasca Paola , Mayeda Kevin 
Published: May 2023

Abstract
As part of the community stress-drop validation study, we evaluate the uncertainties of seismic moment M0 and corner frequency fc for earthquakes of the 2019 Ridgecrest sequence. Source spectra were obtained in the companion article by applying the spectral decomposition approach with alternative processing and model assumptions. The objective of the present study is twofold: first, to quantify the impact of different assumptions on the source parameters; and second, to use the distribution of values obtained with different assumptions to estimate an epistemic contribution to the uncertainties. Regarding the first objective, we find that the choice of the attenuation model has a strong impact on fc results: by introducing a depth-dependent attenuation model, fc estimates of events shallower than 6 km increase of about 10%. Also, the duration of the window used to compute the Fourier spectra show an impact on fc: the average ratio between the estimates for 20 s duration to those for 5 s decreases from 1.1 for Mw&lt;3 to 0.66 for Mw&gt;4.5. For the second objective, we use a mixed-effect regression to partition the intraevent variability into duration, propagation, and site contributions. The standard deviation ϕ of the intraevent residuals for log(fc) is 0.0635, corresponding to a corner frequency ratio 102ϕ=1.33. When the intraevent variability is compared to uncertainties on log(fc), we observe that 2ϕ is generally larger than the 95% confidence interval of log(fc), suggesting that the uncertainty of the source parameters provided by the fitting procedure might underestimate the model-related (epistemic) uncertainty. Finally, although we observe an increase of log(Δσ) with log(M0) regardless of the model assumptions, the increase of Δσ with depth depends on the assumptions, and no significant trends are detected when depth-dependent attenuation and velocity values are considered.
Risk perception normalization of sunlight exposure
Authors: Gaspar Gabriela , Luís Sílvia 
Published: May 2023

Health organizations recommend avoiding direct sunlight exposure usually between 11 am and 5 pm. Nevertheless, it is common to see people on the beach during all day. This study focuses on understanding if sunlight exposure risk might be normalized. Risk normalization is a process by which people minimize a perceive threat to psychologically cope with it, frequently by using positive illusions. A pilot study (N = 44) suggests that the positive illusions most referred to explain exposition at unrecommended hours are the use of protective measures. To explore if knowledge of sunlight exposure risk and risk perception were negatively associated, illustrating risk normalization, a questionnaire was applied to a convenience sample (N = 276). This effect was found among those individuals that exposed themselves to sunlight between 12 am and 3 pm and reported a use of protective measures that was above the average. Furthermore, health literacy moderated the negative relation between knowledge and risk perception. This study suggests that it is important communicating that the use of protective measures during unrecommended hours does not guarantee protection, promoting health literacy, as it can minimize risk normalization, and understanding how information on risks and benefits interacts to influence risk perception.
FACTORS AFFECTING ADEQUATE MYOCARDIAL PERFUSION IN PATIENTS WITH ACUTE ST-ELEVATION MYOCARDIAL INFARCTION WITH SUCCESSFUL EPICARDIAL FLOW
Authors: AYDINYILMAZ Faruk , ÖZBEYAZ Nail Burak , ALGUL Engin , GULIYEV İlkin , ŞAHAN Haluk Furkan , ÖZKAYA İBİŞ Ayşenur , KALKAN Kamuran , SUNMAN Hamza 
Published: May 2023

Aim: The aim of this study was to evaluate and compare multifarious parameters between complete and incomplete ST-segment resolution (STR) patients groups and to identify associates of STR in patients with acute ST-segment elevation myocardial infarction (STEMI) after successful primary percutaneous coronary intervention (pPCI).
Material and Methods: 888 consecutive patients were divided into two groups according to the STR
Acil Servise Başvuran COVID-19 Hastalarında Mukosiliyer Klirensin İncelenmesi
Authors: UZUNGET Sinan Cem , ERTÜRK Zamir Kemal , BAYRAK Sevinç , ÜNAL Hidayet Can , TÜRKKANI Mustafa Hamidullah 
Published: May 2023

Öz
Solunum yolu epitelinde yer alan mukosiliyer tabaka, solunum yollarındaki ilk koruma mekanizması olarak görev yapmaktadır. Şiddetli akut solunum sendromu koronavirus-2 de (SARS-Cov-2), diğer bazı solunum yolu virüsleri gibi siliyer hücrelere yapışmakta ve mukosilier aktiviteyi etkilemektedir. Sakkarin testi ile mukosilier klirensin ölçülmesi mümkündür. Çalışmamızda acil servise başvuran hastalarda SARS-CoV 2 ye bağlı olarak gelişen Korona virus hastalığı-2019 (COVİD-19) enfeksiyonunda erken dönemde mukosiliyer klirensin etkisini değerlendirmeyi amaçladık
Gereç ve Yöntem:
Acil servise başvuran ve son 48 saat içerisinde COVID-19 düşündüren yakınmaları olan ve Polimeraz zincir reaksiyonu (PCR) testi çalışılan 84 kişilik hasta grubunda mukosilier klirens zamanı (MCT), sakkarin testi ile ölçülerek sağlıklı gönüllülerde yapılan ölçüm ile karşılaştırılmıştır.
Sonuçlar
PCR pozitif olarak tespit edilen katılmcılarda ortalama MCT 14.58 dakika olarak, sağlıklı gönüllülerde ise ortalama MCT 13.72 dakika olarak ölçüldü. İki grup arasında istatistiksel açıdan fark saptanmadı (p=0,657).
Tartışma
COVID-19 etkeni olan SARS-CoV-2’nin solunum yolu epiteli hasarı yaptığı ve solunum yollarında hastalık oluşturduğu bilinmektedir. Erken dönemde yapılan PCR testlerinde üst solunum yolu mukozasında tespit edilmekle beraber, erken dönemde MCT üzerine etkisi bulunmamaktadır.
Peran Politik Tokoh Agama pada Pemilu
Authors: Amin Muhammad , Izomiddin Izomiddin , Anisya Siti 
Published: May 2023

ABSTRAK
Kajian tentang tokoh agama dan juga perananya dalam politik selalu menarik untuk dibahas.Karena tokoh agama sendiri memiliki kharismatik dan juga pengetahuan agama yang sangat melekat pada dirinya, sehingga masyarakat selalu mendiskusikan permasalahan keagamaan dan juga permasalahan lainnya termasuk politik. Dan juga menggunakan atau memanfaatkan figur Tokoh Agama untuk memenangi dalam pemilu adalah sah-sah saja, asal tokoh agama itu sendiri bisa memposisikan diri sebagai pengarah ke arah yang lebih positif dan lebih baik. Juga yang lebih penting peran tokoh agama atau kyai dalam percaturan kancah politik tidak melupakan tujuan mulia yaitu mengajak masyarakat untuk sadar menggunakan hak pilihnya yang tentunya untuk memilih pemimpin yang amanah. Adapun tujuan dari penelitian ini adalah untuk mengetahui peran tokoh agama Pada Pemilu tahun 2019. Adapun metode yang digunakan dalam penelitian ini adalah penelitian kualitatif. Peran yang dilakukan oleh tokoh agama dalam pemenangan pemilu tentunya tidak terlepas dari otoritas kharismatik yang dimiliki serta melalui perangkat peran dan fasilitas peran. Terdapat 3 peran yang dilakukan oleh para tokoh agama dalam perananya Pertama, berperan sebagai pembentuk opini di dalam masyarakat. Kedua, berperan sebagai penggerak massa, dan ketiga, berperan sebagai orang yang berkampanye.
Kata kunci: tokoh agama, pemilu, politik.
Upaya Meningkatkan Kualitas Pendidikan melalui Peran Kepala Sekolah
Authors: Mahdali Fitriyah , Abdulloh Abdulloh 
Published: May 2023

The focus of this research is emphasized on the quality of education at SMK Muhammadiyah 7 Gondanglegi, which is about the principal's efforts to improve the quality of education.There are several arguments that form the basis of this research, first, this school is included one of the leading Vocational High Schools (SMK) in Malang Regency. Second, although this school is relatively new compared to other schools in Gondanglegis sub-district is able to provide quality, quantity, even facilities exceed other schools in Gondanglegi.And third, this school has issuing high quality graduates (outputs) where these graduates are now widely scattered in several colleges. There is a limitation of the problem in this study so that the problem formulation is concluded which was taken by researchers to conduct this research, namely (1) How is the effort of the head schools in improving the quality of education at Muhammadiyah 07 Gondanglegi Vocational High School (SMK) Malang (2) How is the success of the principal in improve the quality of education in Muhammadiyah Vocational High School (SMK) 07 Gondanglegi Malang (3) How is the success of the principal in improving the quality of education in Muhammadiyah 07 Vocational School (SMK) Gondanglegi Malang. The method used in this research is descriptive qualitative, namely one a type of research that aims to describe and understand certain phenomena.Method data collection used is by means of documentation and interviews.The findings of this study are (1) the principal's efforts to improve the quality of education which is done in many ways.(2) obstacles faced by school principals as well solutions / policies in improving the quality of education (3) The success of the principal in improving the quality of education, in this case the researcher reveals the success this by describing the achievements and reputations that were obtained and held by the SMK Muhammadiyah 07 Gondanglegi during the last four years in the leadership of Bpk H. Pahri, S.Ag., MM.

Keywords: Education; quality; principal
Co-culturing Chlorella vulgaris and Cystobasidium oligophagum JRC1 in the Microbial fuel cell (MFC) cathode for lipid biosynthesis.
Authors: Mishra Akanksha , Chhabra Meenu 
Published: May 2023

Abstract
This present study investigated the effect of co-culturing the photobiont and mycobiont in the microbial fuel cell (MFC) cathode for lipid generation. Chlorella vulgaris provides oxygen and nutrients for the yeast Cystobasidium oligophagum JRC1 while latter provides CO2 and quench oxygen for higher algae growth. Co-culture enhanced the lipid output of biomass by 28.33%. The total lipid yield and productivity with co-culture were 1.47 ± 0.18 g/L and 0.123 g/L/day respectively. The MFC attained open circuit voltage of 685 ± 11 mV. Synthetic wastewater was used at the anode with sodium acetate as a substrate. The power density of the system was 5.37 ± 0.21 mW m-2 with 75.88 ± 1.89% of COD removal. The total energy output (Lipid + Electrical energy) from the co-culture MFC was 11.5 ± 0.035 kWh m-3 which was 1.4-fold higher than algae alone.