bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Analysis of the NAC Gene Family and Functional Verification of the DcNAC043s in Dendrobium catenatum
Authors: Liu Miao , Tian Tian , Li Yong-quan , Tang Liu , Tian Yu-hang , zhang Yu-jin , shangguan Li-yang , Hang Ye , Huang Ming-jin , zhang Ming-sheng 
Published: May 2023

Abstract
The NAC (NAM, ATAF1/2 and CUC2) domain protein plays an important role in plant growth and development. The function of members of NAC gene family has been deeply studied in many plants. However, the evolutionary relationships and characteristics of NAC family genes in Dendrobium catenatum (D.candidum) unclear. In this study, we identified 33 NAC genes in D.catenatum, all contain NAM conservative domain. Subcellular localization predictions indicated that all the DcNAC proteins are localized to the nucleus. Phylogenetic analysis suggested that the DcNAC gene family could be divided into four groups. Then, the amino-acid composition, physicochemical properties, gene structure, motif, and promoter cis-acting elements were analyzed, the evolutionarily conservative gene DcNAC043 was found. Using qRT-PCR, DcNAC043-1 and DcNAC043-2 were proved to respond to drought stress induction in D.catenatum. At the same time, phloroglucinol staining proved that the expression of DcNAC043-1 and DcNAC043-2 in the plant were up-regulated, which could increase the lignin content in the plant. We supplemented the relevant information of NAC gene family in D.catenatum. At the same time, the gene function of DcNAC043s and its contribution to the response of Dendrobium to drought stress were verified. These results provide a comprehensive evolutionary history of NAC genes in D.catenatum, and insight into the biological functions of DcNAC043s genes in response to drought stress.
Assessing the impact of an evidence- and consensus-based guideline for controlling SARS-CoV-2 transmission in schools on decision-making processes
Authors: Wabnitz Katharina , Rueb Mike , Rehfuess Eva A. , Strahwald Brigitte , Pfadenhauer Lisa M. 
Published: May 2023

Abstract
Introduction

During the COVID-19 pandemic, decision-making on measures to reduce or prevent transmission of SARS-CoV-2 in schools was rendered difficult by a rapidly evolving and uncertain evidence base regarding their effectiveness and unintended consequences. To support decision-makers, an interdisciplinary panel of scientific experts, public health, and school authorities as well as those directly affected by school measures, convened in an unprecedented effort to develop an evidence- and consensus-based public health guideline. This study sought to assess the extent to which this guideline impacteddecision-making processes.

Methods

This study comprised three components: 1) We sent inquiries according to the Freedom of Information Acts of each federal state to ministries or departments of education, family, and health. 2) We conducted semi-structured interviews with individuals involved in decision-making regarding school measures in two federal states. 3) We conducted semi-structured interviews with members of the guideline panel. The content of response letters in component 1 was analysed descriptively; data for components 2 and 3 were analysed using deductive-inductive thematic qualitative content analysis according to Kuckartz.

Results

Responses to the Freedom of Information Act inquiries showed that the guideline was recognised as a relevant source of information by ministries of education in nine federal states and used as a reference to check existing directives for school measures in five federal states. It was consulted by participants who advised policymakers (5 interviews). Overall, perceptions regarding the guideline’s impact were, however, mixed. All participants (20 interviews) discussed the value of the guideline given its evidence- and consensus-based development process but also noted limitations in its usability and usefulness, e.g., lack of context-specificity.

Conclusions

Our findings suggest that the guideline was widely known in federal decision-making bodies, but that its actual use was relatively limited. We suggest that further research to evaluate the impact of public health guidelines on (political) decision-making is warranted, and that guideline development processes may need to be adapted to account for the realities of decision-making during public health emergencies and beyond.
Spatial evaluation and zoning strategy of land use elemental conflicts in heavy industrial zones: Evidence from central Liaoning province in northeast China
Authors: Li Wenying , Wang Yue , Jiang Yuting , Liu Zhaoyu , Shen Dianshi 
Published: May 2023

Abstract
The matching imbalance of resource factors leads to land use elemental conflicts (LUECs), which has become the bottleneck restricting high-quality social and economic development. The heavy industrial zones (HIZ) have become the focus area of LUECs due to the high resource consumption. Taking the urban group of central Liaoning Province, the area of industrial revitalization in northeast China as a case study area, the study proposed a wavelet coherence approach to identifying the influencing indicators and indicators weight of LUECs for spatial evaluation. Two‒dimensional graph theory is used to cluster the evaluation results of LUECs at the plot scale, and controls the main indicators to put forward the zoning strategies of LUECs. The results showed that the main indicators affecting LUECs in the western part of the HIZ are mainly human indicators, while the fierce conflicts in the east mainly come from natural indicators. The zoning strategies of LUECs in the HIZ should prevent excessive energy consumption from increasing carbon emissions in intense conflicts zone and moderate conflicts zone, strengthen the rural settlement arrangement and soil erosion control in mild conflicts zone, and structure ecological security early warnings in potential conflicts zone. This study provides an important reference for land use conflicts in the global heavy industrial urban agglomeration.
Swedish musculoskeletal researchers view on a collaborative network and the most important future research areas in Swedish health care: A SweMSK national collaborative network study
Authors: Diarbakerli Elias , Thoreson Olof , Björklund Martin , Leif Dahlberg , Englund Martin , Gerdhem Paul , Kvist Joanna , Mohaddes Maziar , Peolsson Anneli , Rolfson Ola , Öberg Birgitta , Abbott Allan 
Published: May 2023

Abstract

Background Musculoskeletal (MSK) disorders are a global burden causing significant suffering and economic impact. Systematic identification and targeting of research questions of highest interest for stakeholders can aid in improving MSK disorder knowledge and management. Therefore, this study aims to obtain Swedish MSK researchers' opinions and views on a collaborative Swedish MSK network (SweMSK) and identify future research areas of importance for Swedish MSK research. Methods A web-based survey was conducted from July to September 2021 to collect data from Swedish MSK researchers. The survey focused on the need, objectives, and structure of a SweMSK network and identified priority and key areas for future MSK research. Results The study included 140 respondents, of which 82 were associate professors or professors. The majority (68%) supported the creation of a new musculoskeletal network. The most supported element was increased collaboration regarding nationwide and multicenter studies. Respondents recommended the creation of a homepage and the establishment of national work groups with different specific interests as the primary elements of a new network. Discussion The results demonstrated a need and desire for increased national research collaboration and the creation of a new musculoskeletal network. The high academic experience and active research participation of the respondents suggest the need for MSK disorder knowledge and management improvement in Sweden. Therefore, the SweMSK network may help to facilitate effective collaboration and research efforts that can contribute to the advancement of MSK disorder management and care. This study may provide valuable insights for policymakers, clinicians, and researchers to improve MSK disorder care and management in Sweden.
Levels of organic phosphorus, the pollution status, and the potential health risks in Urumqi's snow water in the years 2019-2022
Authors: Zhaoyong Zhang , Jieyi Guo , Pengwei Wang 
Published: May 2023

Abstract
Due to the lack of research on organic pollutants in snow water and the rapid economic growth of oasis cities in dry areas, this study looked at the pollution characteristics and sources of organophosphorus flame retardants in the urban snow of Urumqi, a typical oasis city. The results showed that: (1) The average (mean) values of 10 organophosphorus flame retardants detected in the snow of Urumqi from 2019 to 2022 were TMP-50.09(45.52) ng/L, TEP-41.79(39.75) ng/L, TnBP - 41.48(43.32) ng/L, TEHP - 69.19(65.64) ng/L, TCEP - 64.64 (61.21)ng/L, TCPP-78.20(72.32) ng/L, TDCPP-140.62(132.22) ng/L, TCP-28.61(25.67) ng/L, TPhP - 29.83(31.22) ng/L, and EHDPP-6.21(8.65) ng/L, respectively. (2) According to an analysis of the single factor pollution index and comprehensive pollution index, the single factor pollution index values for TCEP, TCPP, and TDCPP in 2020, TCPP and TDCPP in 2021, and TCEP, TCPP, and TDCPP in 2022 are all larger than 1, which results in pollution. 10 organophosphorus flame retardants' complete pollution index values were found in the snow in Urumqi. The composite index value for January, February, and December of 2019 was 4.56, which is considered to be extremely high pollution. The overall index value of 3.16 in January, February, and December of 2022 indicated severe pollution, whereas other years belonged to low to moderate pollution levels. (3) The health risk assessment of organophosphorus flame retardants in snow showed that the ADDs of 10 OPEs ranged from 29.87 to 34.22 ng/(kg·d) under the average exposure level, and from 30.92 to 48.39 ng/(kg·d) under the high exposure level. The ADDs of 10 OPEs were much below the threshold for carcinogenic and non-carcinogenic risk computation, falling between 1.00×10-2 and 10-6 range. (4) Based on PCA and correlation analysis, we know that PC1 (TBOEP, TCPP, TCEP, TDCPP and EHDPP) is primarily from atmospheric migration and dry and wet deposition; PC2 (TMP, TEP and TPhP) mainly comes from VC materials and thermoplastic synthetic rubber; PC3 (TnBP and TCP) largely comes from atmospheric transport and dry and wet deposition.
The first report of Pyrenochaetopsis terricola as a dark septate endophytic fungus antagonistic to Fusarium oxysporum f. sp. Lycopersici, a pathogen causing wilt disease in tomato plants
Authors: Surono Surono , Zaffan Zuhay Ratuz , Soekarno Bonny Poernomo Wahyu , Munif Abdul , Nurdebyandaru Nicho 
Published: May 2023

Abstract
In tropical areas like Indonesia, there hasn't been much research on dark septate endophyte fungi (DSE), particularly to take advantage of its function as a plant disease controller. The aim of this study was to investigate if DSE isolates DS08-Ib and DS08-Ic could control Fusarium wilt in tomatoes. Molecular analysis revealed that those isolates are related to Pyrenochaetopsis terricola. Siderophores and hydrolytic enzymes such as catalase and glucanase were produced by P. terricola DS08-Ib and DS08-Ic. The P. terricola DS08-Ic was capable of producing chitinase, whereas P. terricola DS08-Ib was not. In the nursery system, tomato plant inoculated with P. terricola DS08-Ib and DS08-Ic and challenged with Fol had a lower disease incidence than the control, at 37.04% and 37.04%, respectively, whereas the disease incidence in the control was 92.59%. Under Fol challenge, Inoculation of DS08-Ib and DS08-Ic increased tomato seedling dry weight by 37.5% and 33.3%, respectively, and plant height by 32.6% and 18.5%, respectively, compared to the control treatment. Plants inoculated with P. terricola DS08-Ib and DS08-Ic produced a higher peroxidase response than the control under Fol challenge. The two DSE fungi colonized the roots of tomat seedling in the nursery system. In this study, we found that P. terricola has DSE characteristics and could suppress Fusarium wilt of tomato. In the future, the two DSE fungi could help to promote sustainable agriculture by reducing pesticide use to suppress Fusarium wilt in tomato plants.
RNF115/BCA2 deficiency alleviated acute liver injury in mice by promoting autophagy and inhibiting inflammatory response
Authors: Feng Jinqiu , Ye Shufang , Lou Yaxin , Duan Mengyuan , Guo Pengli , Lv Ping , Lu Wenping , chen yingyu 
Published: May 2023

Abstract
The E3 ubiquitin ligase RING finger protein 115 (RNF115), also known as breast cancer-associated gene 2 (BCA2), has been linked with the growth of some cancers and immune regulation, which is negatively correlated with prognosis. Here, it is demonstrated that the RNF115 deletion can protect mice from acute liver injury (ALI) induced by the treatment of lipopolysaccharide (LPS)/D-galactosamine (D-GalN), as evidenced by decreased levels of alanine aminotransaminase, aspartate transaminase, inflammatory cytokines (e.g., tumor necrosis factor α and interleukin-6), chemokines (e.g., MCP1/CCL2) and inflammatory cell (e.g., monocytes and neutrophils) infiltration. Moreover, it was found that the autophagy activity in Rnf115−/− livers were increased, which resulting in the removal of damaged mitochondria and the hepatocyte apoptosis. However, the administration of adeno-associated virus Rnf115 or autophagy inhibitor 3-MA impaired autophagy and aggravated liver injury in Rnf115−/− mice with ALI. Further experiments proved that RNF115 interacts with LC3B, negatively regulates LC3B protein homeostasis and cell autophagy. Additionally, Rnf115 deletion inhibited M1 type macrophage activation via NF-κB and Jnk signaling pathways. Elimination of macrophages narrowed the difference in liver damage between Rnf115+/+ and Rnf115−/− mice, indicating that macrophages were linked in the ALI induced by LPS/D-GalN. Collectively, for the first time, we have proved that Rnf115 inactivation ameliorated LPS/D-GalN-induced ALI in mice by promoting autophagy and attenuating inflammatory responses. This study provides new evidence for the involvement of autophagy mechanisms in the protection against acute liver injury.
Assessment of air-sand-biomass flow and particle segregation in a conical spouted bed using CFD
Authors: Araujo Maynne Soares , de Faria Erica Victor , Bacelos Marcelo Silveira , Xavier Thiago Padovani , Barrozo Marcos Antonio de Souza , Lira Taisa S. 
Published: May 2023

Abstract
Biomass pyrolysis is a promising technology used to obtain important industrial chemicals or to produce renewable energy. For pyrolysis reaction, conical spouted beds (CSBs) can be used because of the high heat and mass transfer rates. On the other hand, for CSB either the fluid dynamics stability or particle segregation may limit its usage as a reactor. As a contribution to the application of CSB reactors for biomass pyrolysis, this research aims not only to test Euler-Euler models to predict segregation in CSBs composed of binary mixtures with different particle shapes and densities but also present experimental verification. The computational fluid dynamic (CFD) designs are carried out using the Euler granular model. The results reveal that the CFD simulation using Gidaspow's drag and dispersed k-ε turbulence models can better represent the experimental pressure drop and axial biomass particle concentration in the bed.
Forecasting system in decision-making on the chemical control of Asian soybean rust
Authors: Engers Lana Bruna de Oliveira , Radons Sidinei Zwick , Henck Aline Ulzefer , Novakowiski Jaqueline Huzar , Bortoluzzi Mateus Possebon 
Published: May 2023

Abstract
Asian soybean rust (ASR; Phakopsora pachyrhizi) is one of the most important diseases of soybean that can reduce soybean yield. The frequent use of site-specific fungicides has led to the evolution of resistant populations of the pathogen, resulting in reduced effectiveness of chemical control. Thus, to reduce the number of fungicide sprays in the crop, disease forecasting systems are tools that could help in the decision-making process about the time for fungicide application. The aim of this study was to develop and validate a forecasting system for ASR to guide chemical control. Four experiments were carried out in three locations (Cerro Largo, Entre Ijuís and Passo Fundo) in the state of Rio Grande do Sul in southern Brazil. Treatments consisted of intervals of calculated severity values (CSV) between fungicide applications, in addition to control without application. Thousand-grain mass and yield (kg ha-1) were evaluated in addition to ASR severity. The data were subjected to analysis of variance using the F test and the means compared using the Tukey’s test at 5% error probability level. The results showed that the disease forecasting system allowed the yield maintenance in different locations and crop seasons, associated with a decrease in the number of fungicide sprays. Thus, we conclude that the proposed disease forecasting system is a viable tool for ASR management.
Prediction of blood pressure changes during surgical incision using minimum evoked current of vascular stiffness value under sevoflurane anesthesia
Authors: Shorin Daiki , Kamiya Satoshi , Nakamura Ryuji , Ishibashi Ayaka , Saeki Noboru , Tsuji Toshio , Tsutsumi Yasuo M. 
Published: May 2023

Abstract
Opioid sensitivity is difficult to predict, and varies among individuals. We previously reported that sympathetic responses to nociceptive stimuli under propofol anesthesia can be predicted by measuring the minimum evoked current of the vascular stiffness value (MECK). We now propose that MECK can also be used in the same capacity for volatile anesthetic. Thirty patients undergoing laparotomy with sevoflurane anesthesia received intravenous remifentanil at a constant concentration of 2 ng/mL followed by tetanic stimulation. The first-order linear regression equation of MECK and the rate of change of systolic blood pressure during the skin incision (ROCBP) under sevoflurane anesthesia were almost the same as those for propofol. We fitted the data in the present study to the prediction equation for ROCBP obtained under propofol anesthesia and performed a Bland-Altman plot analysis. Fixed bias was minimal, at -1.86%, and the precision range at 9.96% was almost the same as 10.17% that from the previous study. No significant proportional errors were observed. These results suggest that MECK can be used as a predictive index for ROCBP under sevoflurane anesthesia. Additionally, the prediction equation for ROCBP under propofol anesthesia can be used for sevoflurane anesthesia without a decrease in predictive performance.
Clinical trial registration Registry: University hospital medical information network; Registration number: UMIN000047425; principal investigator name: Noboru Saeki; date of registration: April 8th, 2022.
Educational intervention for the promote behaviors related to oral health: a randomized controlled trial protocol
Authors: marashi Seyedeh Zahra , Heidarnia Alireza , Kazemi Seyedeh Somayeh , zarei fatemeh 
Published: May 2023

Abstract
Introduction
Considering that Oral health is an essential part of the overall health in infants, children, and adolescents; and on the other hand, nowadays; many adolescents use mobile applications to monitor their lifestyle behaviors and improve their health. Therefore, the present study will be conducted with the aim of the effect of Mobile App intervention based on the theory of self-efficacy on the promotion of behaviors related to oral health of adolescents.
Materials and Methods
This is a randomized controlled trial that will be done in two steps. The first stage is related to a descriptive-analytical study; at this stage, determination the current status and educational need assessment of behaviors related to oral health and will be reviewed eligible individuals. The second stage is an experimental study. At this stage, eligible adolescents are divided into two groups of intervention and control based on randomized allocation. Then the interventions will be implemented by using of educational strategies based on the self-efficacy theory through the mobile application. Evaluations will be performed with a standard questionnaire of self-efficacy and behaviors related to oral health (knowledge, attitude, behavior).
Discussion
Adolescents who are in the early stages of permanent teeth; and this unfavorable condition of the mouth and teeth can have negative effects on the longevity of permanent teeth.
Trial Registration
Iranian Registry of Clinical Trial (IRCT) IRCT20200120046204N1. Registered on 24 September 2022 Ethics code: IR.MODARES.REC.1401.125
Trial registration
(IR.MODARES.REC.1401.125)
Peripheral circulatory support and pericardial drainage for cardiac tamponade from blowout rupture: A case report
Authors: Kato Taichi , Miyagawa Atsushi , Hikone Mayu , Yuri Koichi , Sugiyama Kazuhiro 
Published: May 2023

Abstract
Background
Left ventricular free wall rupture is one of the most lethal complications of myocardial infarction and can cause catastrophic cardiac tamponade. Extracorporeal membrane oxygenation (ECMO) is often used to treat hemodynamic instability due to cardiac tamponade. However, elevated pericardial pressure collapses the right atrium, resulting in inadequate ECMO access and preventing stabilization of the circulation. Further, it interferes with the venous return from the superior vena cava (SVC), which increases the intracranial pressure and reduces cerebral perfusion levels.
Case presentation:
A 65-year-old man was hospitalized for out-of-hospital cardiac arrest. We used ECMO for cardiopulmonary resuscitation. After the establishment of ECMO, transthoracic echocardiography and left ventriculography revealed massive pericardial effusion. The treatment was supplemented with pericardial drainage since ECMO failed frequently in providing adequate suction. However, the blowout rupture caused the requirement of constant drainage from the pericardial catheter. Herein, we connected the venous cannula of ECMO and pericardial drainage catheter. The surgery was performed with stable circulation without the suction failure of ECMO.
Conclusion
We present a case wherein the combination of pericardial drainage and ECMO was used to maintain circulation in a patient with massive pericardial effusion due to cardiac rupture.