bluuebunny/crossref_metadata_2025_split huggingface dataset:
Team Approach: Use of Opioids in Orthopaedic Practice
Authors: Pasqualini Ignacio , Rullán Pedro J. , Deren Matthew , Krebs Viktor E. , Molloy Robert M. , Nystrom Lukas M. , Piuzzi Nicolas S. 
Published: March 2023

» The opioid epidemic represents a serious health burden on patients across the United States.» This epidemic is particularly pertinent to the field of orthopaedics because it is one of the fields providing the highest volume of opioid prescriptions.» The use of opioids before orthopaedic surgery has been associated with decreased patient-reported outcomes, increased surgery-related complications, and chronic opioid use.» Several patient-level factors, such as preoperative opioid consumption and musculoskeletal and mental health conditions, contribute to the prolonged use of opioids after surgery, and various screening tools for identifying high-risk drug use patterns are available.» The identification of these high-risk patients should be followed by strategies aimed at mitigating opioid misuse, including patient education, opioid use optimization, and a collaborative approach between health care providers.
Further Insights into Deep Structure of Malmyzh, Pony-Muli, and Anadzhakan Ore Clusters in the Middle Amur Sedimentary Basin (Northern Sikhote-Alin Orogenic Belt)
Authors: Didenko A.N. , Nosyrev M.Yu. , Gil’manova G.Z. 
Published: March 2023

Abstract
—Magnetic and density depth models were calculated for the Malmyzh, Pony-Muli, and Anadzhakan porphyry-copper ore clusters emplaced in the northern part of the Middle Amur sedimentary basin. Based on analysis of anomalous magnetic field (magnetic model) data, a magmatogenic ring structure determining spatial patterns in localization of porphyry-copper mineralization in the Malmyzh and Pony-Muli ore clusters was established. It is shown that similar structures in the anomalous magnetic field are reported for many porphyry copper deposits. The area of the identified geologic structure can be considered as a high-potential region for detection of goldcopper-porphyry ore mineralization. Within the ring structure, perspective areas containing granitoid and diorite intrusions developed along its periphery and seated beneath the Middle Amur basin sedimentary cover can also be identified. Geophysical depth models are calculated for individual ore clusters with Au–Cu porphyry mineralization, to determine subsurface lithology and structures that may be useful for assessing regional ore potential and deducing areas for metallogenic exploration. The geophysical data-based deep subsurface model for the Malmyzh ore cluster is found to be consistent with the integrated petrological models developed by Richards and Sillitoe for giant Cuporphyry deposits.
Chemically defined cytokine-free expansion of human haematopoietic stem cells
Authors: Sakurai Masatoshi , Ishitsuka Kantaro , Yamazaki Satoshi 
Published: March 2023

Abstract

The ex vivo expansion of human hematopoietic stem/progenitor cells (HSPCs) has important applications in both basic research and clinical transplantation therapy. Here, we introduce the protocol of a novel culture system that supports the long-term ex vivo expansion of human HSPCs, achieved through the complete replacement of cytokines and albumin by chemical agonists and a caprolactam-based polymer. A phosphoinositide 3-kinase activator in combination with a thrombopoietin receptor agonist and the pyrimidoindole derivative UM171 were sufficient to stimulate functional expansion of umbilical cord blood-derived HSCs. We envision that this chemically defined expansion culture system will help to advance clinical HSC therapies.
Reflections on the Implementation of Wearable Vital Signs Monitors in a Vietnamese ICU
Authors: McKnight Jacob , Luu An Phuoc , Nuil Jennifer Van , Phan Khanh Nguyen Quoc , Hoang Van Minh Tu , Phung Nhat Tran Huy , Nguyen Tung Le Nhu , Nguyen Hao Van , Dao Khoa Bach , Ho Hai Bich , Le Khanh Thuy Thuy , Nguyen Huy Quang , Lam Yen Minh , Truong Trung Ngoc , Nguyen Truong Thanh , Nguyen Chau Van Vinh , Thwaites Louise 
Published: March 2023

Abstract
The number of patients in need of critical care increased sharply in Vietnam during the COVID-19 pandemic putting increased pressure on the Hospital for Tropical Diseases, which was designated as a centre for treatment. In order to meet the new demand for critical care, the hospital introduced a prototype wearable system to monitor the vital signs of COVID patients. We describe how the introduction of the wearables was supported by a group of implementation scientists using an action learning approach. The support of a dedicated implementation team facilitated the implementation process and enabled significant advances in a limited time. We found that while the devices addressed a significant demand for remote monitoring, they also created new types of work. The burden of this work was not shared equally and significant thought was needed to find ways of optimising the use of the system, leading to altered workflows and staff training. We conclude that with the right support and planning however, well designed wearables offer promising opportunities for managing critically ill patients.
An approach to the micro-strain distribution inside nanoparticle structure
Authors: Abdel-Rahman Ahmed Sabry 
Published: March 2023

Abstract
The importance of nanotechnology is increasing day by day, and to allow the nanoparticles to do what we hope, explicit modelling of nanostructures is necessary. Considering the strain inside the nanoparticle is the major subject that changes the point of view to the unique properties of the material on the nanoscale. Williamson-Hall, Stocks-Wilson, Debye-Scherrer, Halder-Wagner, and Size-Strain Plot (SSP) methods are used essentially to insure the material particle size falls at the nano-level, they treat the broadening in the XRD peak as a sum of Gauss and Lorentz diffraction probability functions. In this work, when modelling a nanostructure as a liquid drop where surface tension controls the particle position and strain controls the geometry and spacing of the lattice parameters, the number of the diffraction planes is used instead of the line intensity and shows a Gaussian-like (or Lorentzian-like) function that is investigated with numerical analysis. The model writes an equation about the broadening, peak position, and lattice parameters to estimate the crystalline size and strain exponent. Williamson-Hall, Stocks-Wilson, and Debye-Scherrer can be explained as approximations for this model, and once the negative strain is explained, possible approximations can show Halder-Wagner and SSP another face of the strain distribution model equation.
“…, I don’t think the disease is real, some people have just taken pictures to scare us to take the medications”: A qualitative study exploring perceptions about mass drug administration acceptance for lymphatic filariasis in Ghanaian communities
Authors: Muroka Trusila A , Glozah Franklin N 
Published: March 2023

Abstract
Background
Mass drug administration (MDA) is a means of delivering safe and affordable essential evidence-based interventions based on the principles of preventive chemotherapy, where various populations or sub-population groups are offered treatment without individual diagnosis. Several communities have been provided with MDA (i.e., Ivermectin and Albendazole) with the aim of eliminating Lymphatic Filariasis (LF) from Ghana. Notwithstanding the administration of 13 rounds of MDA from 2005 to 2019, the prevalence of LF as at 2020 in some communities is still high. The aim of this study is to explored perceptions about MDA acceptance for LF in Ghanaian communities.
Methods
A qualitative research design using a descriptive phenomenology and narrative inquiry was used in the study to either purposively or randomly select community members, patients, and community drug distributors in 12 communities in the Bono region of Ghana. In-depth interviews and focus group discussions were used to collect data. Interviews were transcribed verbatim and analyzed using thematic analysis in NVivo 12 software.
Results
The results show that chronic clinical symptoms of LF influenced community members’ perception and acceptance of the MDA intervention. Also, community members perceived that the acceptability and uptake of the MDA intervention could be improved by including patients in the programme, carrying out continuous education, and engaging in social mobilization activities in the communities.
Conclusions
There is an urgent need to educate and engage communities with regard to the critical role MDA intervention can play to eliminate LF from Ghana.
Ecological Toxicity Alleviation of Imazethapyr to Non-target Plant Wheat: Active Regulation Between Auxin and DIMBOA
Authors: Huang Jinye , Li Jun , Chen Hui , Shen Chensi , Wen Yuezhong 
Published: March 2023

Abstract
A new perspective was put forward to alleviate the potential
toxicity of herbicide (R)-imazethapyr ((R)-IM) to
non-target plant wheat by active regulation between auxin and
secondary metabolite
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazine-3(4H)-one
(DIMBOA) in this study. (R)-IM effortlessly disrupted the
balance between wheat growth and defense, which not only reduced
32.4% auxin content in wheat leaves, but also induced 40.7% DIMBOA
accumulation compared to the control group. Transcriptomic results
indicate that restoration of the auxin level in plants promotes the
up-regulation of growth-related genes and increased accumulation of
DIMBOA increases the expression of defense-related genes. Auxin and
DIMBOA are in two divergent paths and alleviate herbicide stress on
wheat primarily through effects on growth and defense,
respectively. Additionally, as a common precursor of both auxin and
DIMBOA, indole adopted a combined growth and defense strategy in
response to (R)-IM toxicity, i.e., both restoring growth
development and enhancing the defense system. It might be possible
to find appropriate ways to regulate the plant growth-defense
balance under herbicide stress by regulating auxin and DIMBOA
levels in plants. Our insight into the interference mechanism of
herbicides to the plant growth-defense system will facilitate the
design of improved strategies for herbicide detoxification.
TrkB phosphorylation in serum extracellular vesicles correlates with cognitive function enhanced by ergothioneine in humans
Authors: Kato Yukio , Ishimoto Takahiro , Yamashita Reiya , Matsumoto Ruri , Matsumoto Satoshi , Matsuo Yusuke , Nakao Shunsuke , Masuo Yusuke , Suzuki Makoto 
Published: March 2023

Abstract
Oral administration of the food-derived antioxidant amino acid ergothioneine (ERGO) results in its efficient distribution in the brain and enhanced cognitive function. However, the effect of ERGO deficiency on cognitive impairment and the underlying mechanisms remain unknown. We revealed that cognitive function and hippocampal neurogenesis were lower in mice fed an ERGO-free diet than in those fed the control diet. Furthermore, ERGO supplementation to achieve the control diet ERGO levels reversed these effects and restored ERGO concentrations in the plasma and hippocampus. The ERGO-induced recovery of cognitive function and hippocampal neurogenesis was blocked by TrkB inhibition, with a concomitant reduction in hippocampal phosphorylated TrkB, suggesting the involvement of TrkB activation in these events. Phosphorylated TrkB was also detected in extracellular vesicles (EVs) derived from serum samples of volunteers who had been orally administered ERGO-containing tablets (5 mg/day for 12 weeks). Importantly, the ratio of serum EV-derived phosphorylated TrkB was significantly higher in the ERGO-treated group than in the placebo-treated group and was positively correlated with both serum ERGO concentrations and several cognitive domain scores from Cognitrax. Altogether, TrkB phosphorylation is involved in ERGO-induced cognitive enhancement, and TrkB phosphorylation levels in serum EVs may quantitatively represent ERGO-induced cognitive enhancement in humans.
Role of the GalNAc-galectin pathway in the healing of premature rupture of membranes
Authors: Chen Jia-Le , Liu Lou , Peng Xin-Rui , Wang Yan , Xiang Xiang , Chen Yu , Xu De-xiang , Chen Daozhen 
Published: March 2023

Abstract

Background: Premature rupture of membranes is an important cause of preterm birth and its associated high infant mortality and morbidity, and is an important public health problem worldwide, but the mechanism is unknown, and clinical treatment is currently mainly conservative, with a lack of effective interventions. We compared the differential metabolites in vaginal secretions between patients with preterm premature rupture of membranes and those with normal pregnancies and screened for the protective substance N-acetyl-d-galactosamine (GalNAc), an important ligand for galectin, which has several biological activities such as anti-inflammatory, antioxidant and in vitro wound healing properties.
Methods: We evaluated the inflammatory response, oxidative stress, proliferation migration, EMT and ECM remodelling of human amniotic epithelial cells (WISH) by ELISA, ROS, transwell, scratch and CCK-8 assays, and examined the expression of PCNA and AKT by Western Blot.
Results: The results showed that GalNAc enhanced galectin production and inhibited cellular inflammation and oxidative stress, promoted cellular EMT and migration, and altered ECM composition. In addition, GalNAc reduced the total and phosphorylated protein levels of AKT enhancing the pro-cell migration ability of GalNAc to stimulate wound healing.
Conclusions: The results showed that GalNAc enhanced galectin production and inhibited cellular inflammation and oxidative stress, promoted cellular EMT and migration, synthesized GAG and altered ECM composition. The GalNAc-galectin pathway may provide a theoretical basis and a new target for the prevention and treatment of premature rupture of membranes.
This study provides a theoretical basis and a new target for the prevention and treatment of premature rupture of membranes.
Modeling of Hydrodynamic parameters of rotating disc contactors (RDCs) using response surface methodology: holdup and drop size
Authors: Mirzaei Shaparak , Ghaemi Ahad , Hemmati Alireza , Shirvani Mansour , Ardestani Fatemeh 
Published: March 2023

Abstract
In this study, the behavior of hydrodynamic parameters was investigated in agitated columns including Asymmetric rotating disc contactor (ARDC), Perforated rotating disc contactor (PRDC), Oldshue-Rushton column (ORC), and Kuhni for three different systems such as butanol-water, n-butyl acetate-water and toluene-water. The effects of various parameters including rotor speed (rpm), dispersed and continuous phase velocities, and interfacial parameters were studied on the dispersed phase holdup and Sauter mean drop diameter. The results showed that the enhancement of rotor speed, and dispersed and continuous phase velocities would enhance the dispersed phased holdup. Among these, PRDC shows the least enhancement of dispersed phase holdup compared to the other three columns. The investigation of Sauter mean drop diameter changes in the columns showed that the droplet size decreases sharply as the rotor speed increases, which this decrease of droplet size is greater in ORC and Kuhni columns than in the other two columns. The results also show that by increasing dispersed phase velocity in the four columns, the droplet size in ORC column is larger than the columns, However, by increasing continuous phase velocity, subtle changes in droplet size are observed, Based on the experimental data, new correlations have been presented to predict Sauter mean drop diameter and dispersed phase holdup for agitated columns by the analysis of the variance (ANOVA). The coefficient of determination (R2) was obtained 0.9612 and 0.9495 for Sauter mean drop diameter and dispersed phase holdup, respectively, indicating a good correlation between the predicted and observed and values of the models.
Importance of nuclear localization signal-fused Cas9 in the production of genome-edited mice via embryo electroporation
Authors: Niinuma Sakura , Wake Yui , Nakagawa Yuki , Kaneko Takehito 
Published: March 2023

Abstract

Previously, to generate genome-edited animals by introducing CRISPR-associated protein 9 (Cas9) into embryos, we developed the Technique for Animal Knockout system by Electroporation (TAKE). Additionally, by fluorescently labeling Cas9, we successfully visualized the Cas9 introduced into the pronuclei of embryos; however, whether Cas9 was introduced directly into the pronuclei by electric pulse or transferred from the cytoplasm by nuclear localization signal (NLS) remained unknown. Herein, we evaluated the localization of Cas9 with (Cas9-NLS) or without NLS (Cas9-noNLS) in mice embryos following electroporation by fusing them with GFP. Furthermore, we visually studied their effects on genome-editing rates in offspring by targeting tyrosinase gene. Fluorescence intensity in pronuclei of Cas9-NLS-electroporated embryos and genome-editing rates of offspring were significantly higher than those of Cas9-noNLS-electroporated embryos. Furthermore, fluorescence in Cas9-NLS-electroporated embryos in which pronuclei had not yet appeared 2.5 h after insemination was observed in the pronuclei of embryos appearing 3.5 h after electroporation. We demonstrated the effective transportation of Cas9 from the cytoplasm to pronuclei by the NLS following TAKE, which resulted in increased genome-editing rates in offspring. The TAKE along with fluorescently labeled nucleases can be used to verify nuclease delivery into individual embryos prior to embryo transfer for efficiently producing genome-edited animals.
A new trypan blue agar plate assay for in vivo evaluation of gut damage in Folsomia candida springtail
Authors: Kwak Jin Il , Kim Lia , An Youn-Joo 
Published: March 2023

Abstract
Although the springtail Folsomia candida is a representative soil test species and a widely used biological indicator, current toxic endpoints are limited and required to expand at the individual level. For instance, there is no simple and efficient method for evaluating gut barrier damage in Folsomia candida except for histopathological evaluation or electron microscopic analysis. Because the evaluation of intestinal tissue damage by environmental pollutants can be linked to the gut microbiome and digestive enzyme results, the present study proposes an efficient method for evaluating the gut damage in springtail using trypan blue dye to visualize adverse effects. The media, staining, and depuration periods were optimized, and the proposed method was validated using F. candida exposed to model chemicals (Cu and Cr). The optimal parameters were determined as 1) 0.1% trypan blue + 0.8% agar + 5% sugar plate for 15 min, followed by 2) depuration on an 0.8% agar + 10% sugar plate for 3 h. This new method is expected to provide a simple and cheap tool for evaluating gut damage in springtails compared with histopathological evaluation or electron microscopic analysis. Furthermore, this method can expand individual-level toxic endpoints for springtails.
Exploration of the Shared Gene Signatures and Molecular Mechanisms Between Breast Cancer and Endometriosis
Authors: Hai Linyue , Cao Xuchen , Xiao Chunhua 
Published: March 2023

Abstract
Background
Globally, breast cancer (BC) is the most common type of cancer and the second leading cause of cancer-related deaths among women. Early diagnosis and survival outcomes will be improved through the identification of modifiable risk factors and the development of better disease management strategies. There are many similarities between endometriosis and breast cancer, both in terms of risk factors and developmental characteristics. Thus, it would be beneficial to explore the common mechanisms behind the onset of BC and endometriosis to develop more effective intervention strategies in the future. In this study, bioinformatics was used to explore the key molecules and pathways that mediate the co-occurrence of BC and endometriosis.
Method
From the Gene Expression Omnibus (GEO) database, datasets for BC (GSE15852) and endometriosis (GSE5108) were downloaded. By using the GEO2R online tool, we were able to identify the differentially expressed genes (DEGs) between two diseases. Afterward, a protein-protein interaction network (PPI) was constructed based on DEG enrichment analysis. Additionally, the hub genes were identified using the STRING database and Cytoscape software. We investigated the relationship between hub gene expression levels and clinical expression, pathological stage, age, and prognosis. As a final step, transcription factor interaction, stemness score, and immune cell infiltration analysis were conducted on hub genes in BC.
Results
We identified 33 overlapping DEGs (18 downregulated genes and 15 upregulated genes) for further analysis. The significant functional pathways of DEGs were enriched in regulating the pluripotency of stem cells and the mis-regulation of transcription in cancer. Additionally, five key hub genes were identified, including HOXA10, PAX8, MSX1, FGFR1, and INHBA. Pathological stages, age, stemness score, and immune infiltration were associated with the expression level of hub genes.
Conclusion
A novel insight into the molecular mechanism of endometriosis complicated with BC is provided by the finding that HOXA10, PAX8, MSX1, FGFR1, and INHBA were hub genes for the co-occurrence of BC and endometriosis.
Prognostic Value of Clinical and Computed Tomography Score in Predicting Outcome of Patients with COVID-19: A prospective study in Iran
Authors: Mahdavi Arash , Khalili Nastaran , Dehkordi Saeid Alerasoul , Tajbakhsh Ardeshir , Davarpanah Amir H. , Mahdavi Ali , Zolghadr Zahra , Langroudi Taraneh Faghihi , Taheri Morteza Sanei , Shabestari Abbas Arjmand 
Published: March 2023

Abstract
Background:
Different clinical and radiologic factors predict poor outcomes in patients with Coronavirus Disease 2019 (COVID-19). Thus, we aimed to investigate the performance of two separate clinical and radiologic (CT) scoring systems in detecting the prognosis of patients with COVID-19 using a low-dose protocol for CT imaging.
Methods:
Eighty-six patients with confirmed COVID-19 were included in this prospective study. All patients underwent low-dose chest CT at the initial workup. By evaluating the extent of lung involvement on patients’ initial CT scan, scores from 0 to 4 were assigned to the five lobes and the lingula. Clinical score was based on the following factors: age, sex, presence of comorbidities, respiratory rate, and oxygen saturation. After at least 15 days of follow-up, the disease outcome was classified as either severe (intensive care unit admission, intubation, or death) or favorable. ROC analysis was used to evaluate the ability of each scoring system to predict patients' outcomes.
Results:
After follow-up, 80.2% and 15.1% of cases had developed favorable and severe outcomes (respectively), and 4.7% were lost to follow-up. Those with severe outcomes had a significantly higher clinical score and CT score than patients with favorable outcomes (p < 0.001 and p= 0.012, respectively). The intra-class correlation coefficient value for the CT score was 0.95. The optimal threshold of the CT score for identifying patients with severe outcomes was 7.5 (area under curve= 0.721) with 77% sensitivity and 65% specificity; the clinical score cut-off was 9.25 (area under curve= 0.832) with 92.3% sensitivity and 72.1% specificity.
Conclusions:
Both CT and clinical scoring systems displayed a quick, safe, and objective method for predicting outcomes in patients with COVID-19. However, compared with imaging, stratification of patients based on clinical factors seems to be a stronger predictor of outcome.