bluuebunny/crossref_metadata_2025_split huggingface dataset:
EEG cortical activity and connectivity correlates of early sympathetic response during cold pressor test
Authors: Rho Gianluca , Callara Alejandro Luis , Bernardi Giulio , Scilingo Enzo Pasquale , Greco Alberto 
Published: January 2023

AbstractPrevious studies have identified several brain regions involved in the sympathetic response and its integration with pain, cognition, emotions and memory processes. However, little is known about how such regions dynamically interact during a sympathetic activation task. In this study, we analyzed EEG activity and effective connectivity during a cold pressor test (CPT). A source localization analysis identified a network of common active sources including the right precuneus (r-PCu), right and left precentral gyri (r-PCG, l-PCG), left premotor cortex (l-PMC) and left anterior cingulate cortex (l-ACC). We comprehensively analyzed the network dynamics by estimating power variation and causal interactions among the network regions through the direct directed transfer function (dDTF). A connectivity pattern dominated by interactions in $$\alpha$$
α
(8–12) Hz band was observed in the resting state, with r-PCu acting as the main hub of information flow. After the CPT onset, we observed an abrupt suppression of such $$\alpha$$
α
-band interactions, followed by a partial recovery towards the end of the task. On the other hand, an increase of $$\delta$$
δ
-band (1–4) Hz interactions characterized the first part of CPT task. These results provide novel information on the brain dynamics induced by sympathetic stimuli. Our findings suggest that the observed suppression of $$\alpha$$
α
and rise of $$\delta$$
δ
dynamical interactions could reflect non-pain-specific arousal and attention-related response linked to stimulus’ salience.
Analysis of oxidase activity and transcriptomic changes related to cutting propagation of hybrid larch
Authors: Qin Ruofan , Zhao Qingrong , Gu Chenrui , Wang Chen , Zhang Lei , Zhang Hanguo 
Published: January 2023

AbstractHybrid larch is the main timber and afforestation tree species in Northeast China. To solve the problem of rooting difficulties in larch cutting propagation, enzyme activity determination and transcriptome sequencing were carried out on the rooting tissues at five timepoints after cutting. peroxidase (POD), indole acetic acid oxidase (IAAO) and polyphenol oxidase (PPO) play important roles in the larch rooting process after cutting. A total of 101.20 Gb of clean data was obtained by transcriptome sequencing, and 43,246 unigenes were obtained after further screening and assembly. According to GO analysis and KEGG enrichment analysis, we think that plant hormones play an important role in the rooting process of larch stem cuttings. in the plant hormone signal transduction pathway, a larch gene c141104.graph_c0 that is homologous to the Arabidopsis AUX1 was found to be significantly up-regulated. We suggest that AUX1 may promote IAA transport in larch, thus affecting adventitious root development. According to the results of POD, PPO IAAO indexes and GO analysis, we think s1 and s2 periods may be important periods in the rooting process of larch stem cuttings, so we built a gene regulatory network, a total of 14genes, including LBD, NAC, AP2/ERF, bHLH and etc., may be important in different stages of cutting propagation. As the rooting rate after cutting inhibits the development of larch clone propagation, identifying the genes that regulate rooting could help us to preliminarily understand the molecular mechanism of adventitious root formation and select a better treatment method for cutting propagation.
Investigation of optimal convolutional neural network conditions for thyroid ultrasound image analysis
Authors: Lee Joon-Hyop , Kim Young-Gon , Ahn Youngbin , Park Seyeon , Kong Hyoun-Joong , Choi June Young , Kim Kwangsoon , Nam Inn-Chul , Lee Myung-Chul , Masuoka Hiroo , Miyauchi Akira , Kim Sungwan , Kim Young A. , Choe Eun Kyung , Chai Young Jun 
Published: January 2023

AbstractNeural network models have been used to analyze thyroid ultrasound (US) images and stratify malignancy risk of the thyroid nodules. We investigated the optimal neural network condition for thyroid US image analysis. We compared scratch and transfer learning models, performed stress tests in 10% increments, and compared the performance of three threshold values. All validation results indicated superiority of the transfer learning model over the scratch model. Stress test indicated that training the algorithm using 3902 images (70%) resulted in a performance which was similar to the full dataset (5575). Threshold 0.3 yielded high sensitivity (1% false negative) and low specificity (72% false positive), while 0.7 gave low sensitivity (22% false negative) and high specificity (23% false positive). Here we showed that transfer learning was more effective than scratch learning in terms of area under curve, sensitivity, specificity and negative/positive predictive value, that about 3900 images were minimally required to demonstrate an acceptable performance, and that algorithm performance can be customized according to the population characteristics by adjusting threshold value.
Status does not predict stress among Hadza hunter-gatherer men
Authors: Fedurek Piotr , Lehmann Julia , Lacroix Laurent , Aktipis Athena , Cronk Lee , Makambi E. Jerryson , Mabulla Ibrahim , Berbesque J. Colette 
Published: January 2023

AbstractIn recent years there has been much research regarding the extent to which social status is related to long-term indices of health. The majority of studies looking at the interplay between social status and health have been conducted in industrialized societies. However, it has been argued that most of human evolution took place in small, mobile and egalitarian hunter-gatherer groups where individuals exhibited very little variation in terms of material wealth or possessions. In this study, we looked at the extent to which two domains of social status, hunting reputation (being perceived as a good hunter) and popularity (being perceived as a friend), are related to physiological stress levels among Hadza men, hunter-gatherers living in Northern Tanzania. The results of our study show that neither hunting reputation nor popularity is associated with stress levels. Overall, our data suggest that, in at least some traditional small-scale societies exhibiting an egalitarian social model, such as the Hadza, the variation in social status measures based on both popularity and hunting reputation does not translate into one of the commonly used indices of wellbeing.
Similarities in biomass and energy reserves among coral colonies from contrasting reef environments
Authors: Keister Elise F. , Gantt Shelby E. , Reich Hannah G. , Turnham Kira E. , Bateman Timothy G. , LaJeunesse Todd C. , Warner Mark E. , Kemp Dustin W. 
Published: January 2023

AbstractCoral reefs are declining worldwide, yet some coral populations are better adapted to withstand reductions in pH and the rising frequency of marine heatwaves. The nearshore reef habitats of Palau, Micronesia are a proxy for a future of warmer, more acidic oceans. Coral populations in these habitats can resist, and recover from, episodes of thermal stress better than offshore conspecifics. To explore the physiological basis of this tolerance, we compared tissue biomass (ash-free dry weight cm−2), energy reserves (i.e., protein, total lipid, carbohydrate content), and several important lipid classes in six coral species living in both offshore and nearshore environments. In contrast to expectations, a trend emerged of many nearshore colonies exhibiting lower biomass and energy reserves than colonies from offshore sites, which may be explained by the increased metabolic demand of living in a warmer, acidic, environment. Despite hosting different dinoflagellate symbiont species and having access to contrasting prey abundances, total lipid and lipid class compositions were similar in colonies from each habitat. Ultimately, while the regulation of colony biomass and energy reserves may be influenced by factors, including the identity of the resident symbiont, kind of food consumed, and host genetic attributes, these independent processes converged to a similar homeostatic set point under different environmental conditions.
Resonant laser ionization and mass separation of 225Ac
Authors: Johnson Jake D. , Heines Michael , Bruchertseifer Frank , Chevallay Eric , Cocolios Thomas E. , Dockx Kristof , Duchemin Charlotte , Heinitz Stephan , Heinke Reinhard , Hurier Sophie , Lambert Laura , Leenders Benji , Skliarova Hanna , Stora Thierry , Wojtaczka Wiktoria 
Published: January 2023

Abstract$$^{225}$$

225

Ac is a radio-isotope that can be linked to biological vector molecules to treat certain distributed cancers using targeted alpha therapy. However, developing $$^{225}$$

225

Ac-labelled radiopharmaceuticals remains a challenge due to the supply shortage of pure $$^{225}$$

225

Ac itself. Several techniques to obtain pure $$^{225}$$

225

Ac are being investigated, amongst which is the high-energy proton spallation of thorium or uranium combined with resonant laser ionization and mass separation. As a proof-of-principle, we perform off-line resonant ionization mass spectrometry on two samples of $$^{225}$$

225

Ac, each with a known activity, in different chemical environments. We report overall operational collection efficiencies of 10.1(2)% and 9.9(8)% for the cases in which the $$^{225}$$

225

Ac was deposited on a rhenium surface and a ThO$$_{2}$$

2

mimic target matrix respectively. The bottleneck of the technique was the laser ionization efficiency, which was deduced to be 15.1(6)%.
Identification of prognostic immune-related lncRNA signature predicting the overall survival for colorectal cancer
Authors: Li Jianxin , Han Ting , Wang Xin , Wang Yinchun , Chen Xuan , Chen Wangsheng , Yang Qingqiang 
Published: January 2023

AbstractLong non-coding RNA (lncRNA) is an important regulator of gene expression and serves a fundamental role in immune regulation. The present study aimed to develop a novel immune-related lncRNA signature to assess the prognosis of patients with colorectal cancer (CRC). Transcriptome data and clinical information of patients with CRC were downloaded from The Cancer Genome Atlas (TCGA) and UCSC Xena platforms. Immune-related mRNAs were extracted from the Molecular Signatures Database (MSigDB), and the immune-related lncRNAs were identified based on correlation analysis. Then, univariate, Lasso and multivariate Cox regression were applied to construct an immune-related lncRNA signature, and CRC patients were divided into high- and low-risk groups according to the median risk score. Finally, we evaluated the signature from the perspectives of clinical outcome, clinicopathological parameters, tumor-infiltrating immune cells (TIICs), immune status, tumor mutation burden (TMB) and immunotherapy responsiveness. In total, 272 immune-related lncRNAs were identified, five of which were applied to construct an immune-related lncRNA signature. The signature divided patients with CRC into low- and high-risk groups, the prognosis of patients in the high-risk group were significantly poorer than those in low-risk group, and the results were further confirmed in external validation cohort. Furthermore, the high-risk group showed aggressive clinicopathological characteristics, specific TIIC and immune function status, and low sensitivity to immunotherapy. The immune-related lncRNA signature could be exploited as a promising biomarker for predicting the prognosis and immune status of patients with CRC.
Pharmaceutical toxicity is a common pattern of inpatient acute poisonings in Birjand City, East of Iran
Authors: Naseri Kobra , Kiani Zahra , Sajadi Zakieh Sadat , Mehrpour Omid , Javadmoosavi Seyed Yoosef , Forouzanfar Fatemeh , Sadeghi Mahmood 
Published: January 2023

AbstractInformation on the pattern of acute poisonings in hospitals of Birjand city, Iran, is limited. This study aimed to address this knowledge gap by examining the admissions in a major poisoning center in eastern Iran. This cross-sectional study included patients admitted to the Imam Reza Hospital in Birjand over 12 months. Medical records of the poisoned patients were reviewed, and the study variables were used for data analysis. During the study period, 534 cases of acute poisonings were evaluated. The patient's ages ranged from 12 to 84 years, with a high rate of poisonings between 15 and 35 years. The female predominance in poisoning cases was 52.1%. Most cases of poisonings occurred in spring, and the common route of exposure was oral (93.1%). The incidence of poisoning in married couples, uneducated patients, and residents of urban areas was 56.5%, 90.1%, and 74.6%, respectively. Patients with a previous medical history experienced addiction and psychiatric disorders. Intentional poisoning accounted for 23.4% of acute poisoning cases referred to the hospital in the current study. The main groups of toxicants were pharmaceutical products (48.1%), narcotics (25.8%), chemical products (10.1%), envenomation (7.1%), and alcohol (1.7%). The mean hospital stay was 2.5 ± 3.0 days, and the final treatment outcome was complete recovery, except for one patient intoxicated by warfarin and alprazolam. Our results indicate that the high toxicity cases were related to pharmaceutical product and opioids abuse, especially methadone (8.4%), alprazolam (7.9%), clonazepam (7.5%), and acetaminophen (9.9%) taken orally and more commonly happened at home. Due to the high rate of deliberate poisonings, especially among young adults and students, monitoring drug distribution and exceptional attention to mental health should be seriously considered by national health authorities to prevent suicide attempts.
Expression and significance of IL-6 and IL-8 in canine mammary gland tumors
Authors: Ren Xiaoli , Fan Yuying , Shi Dongmei , Liu Yun 
Published: January 2023

AbstractMammary gland tumors are the most common malignant diseases which seriously threaten the health of women and female dogs. There is a lack of an effective tumor marker which can effectively assist in the early diagnosis, and prognosis of mammary gland tumors in veterinary clinical medicine. IL-6, and IL-8 as immunosuppressive factors may stimulate tumor cells growth, contribute to loco-regional relapse and metastasis that might be utilized as a marker for immunity status and monitoring of the course of tumor. The present study aimed to investigate the expression of serum/tissue IL-6, IL-8 and IL-10 in canine mammary gland tumors using Enzyme linked immunosorbent assay(ELISA), Western blot and Immunohistochemistry assay(IHC) to determine whether it is associated with tumor progression. The results showed that levels of IL-6, IL-8 and IL-10 in serum were higher in malignant tumor group than that in benign tumor and control group; the expression levels of IL-6 and IL-8 were significantly elevated in grade III than in grade I and II and was related to metastasis. Likewise, IL-6 and IL-8 were also highly expressed in malignant tumor tissues. Elevated expression of IL-6 was associated with histopathological grade and metastases in malignant tumors. Moreover, high expression of IL-6 occurred in the Basal-like subtypes whereas high expression of IL-8 occurred in the Luminal B subtypes. The results of this study indicated that changes of IL-6 and IL-8 in the tumor microenvironments were closely related to the diseases status and may be used as a potential diagnostic or biomarker in canine mammary gland tumors.
Experimental validation of proton boron capture therapy for glioma cells
Authors: Shtam Tatiana , Burdakov Vladimir , Garina Alina , Garaeva Luiza , Tran Nhan Hau , Volnitskiy Andrey , Kuus Eva , Amerkanov Dmitry , Pack Fedor , Andreev Georgy , Lubinskiy Andrey , Shabalin Konstantin , Verlov Nicolay , Ivanov Evgeniy , Ezhov Victor , Lebedev Dmitry , Konevega Andrey L. 
Published: January 2023

AbstractProton boron capture therapy (PBCT) has emerged from particle acceleration research for enhancing the biological effectiveness of proton therapy. The mechanism responsible for the dose increase was supposed to be related to proton-boron fusion reactions (11B + p → 3α + 8.7 MeV). There has been some experimental evidence that the biological efficiency of protons is significantly higher for boron-11-containing prostate or breast cancer cells. The aim of this study was to evaluate the sensitizing potential of sodium borocaptate (BSH) under proton irradiation at the Bragg peak of cultured glioma cells. To address this problem, cells of two glioma lines were preincubated with 80 or 160 ppm boron-11, irradiated both at the middle of 200 MeV beam Spread-Out Bragg Peak (SOBP) and at the distal end of the 89.7 MeV beam SOBP and assessed for the viability, as well as their ability to form colonies. Our results clearly show that BSH provides for only a slight, if any, enhancement of the effect of proton radiation on the glioma cells in vitro. In addition, we repeated the experiments using the Du145 prostate cancer cell line, for which an increase in the biological efficiency of proton irradiation in the presence of sodium borocaptate was demonstrated previously. The data presented add new argument against the efficiency of proton boron capture therapy when based solely on direct dose-enhancement effect by the proton capture nuclear reaction, underlining the need to investigate the indirect effects of the secondary alpha irradiation depending on the state and treatment conditions of the irradiated tissue.
Ionomics-metabolome association analysis as a new approach to the impact of dietary copper levels in suckling piglets model
Authors: Zhang Feng , Yao Wen , Ji Xu , Liu Xiaodan , Jin Erhui 
Published: January 2023

AbstractIonomics-metabolomics association analysis is a novel method to elucidating the potential mechanisms underlying the effects of dietary copper on the overall health parameters of suckling piglets model. Few studies have elucidated the relationship between the changes of ionic and metabolic homeostasis responses to dietary copper level. The growth performance data was obtained from 180 suckling piglets which access to different copper levels: 6 (low copper diet, LC), 20 (control diet, CON), and 300 (high copper diet, HC) mg·kg−1 copper (based on diet, supplementation from CuSO4), and offered ad libitum from d 14 until weaning at 40 d of age. Dietary high level copper (300 mg·kg−1) increased the ADG and ADFI during d 14 to 28 of piglets. Six elements (Mg, Na, K, P, Cu, and Mn) concentrations significantly changes in hair among the three treatment diets. The significant increased concentrations of Na and K, and decreased concentration of Mg and Mn in 300 mg·kg−1 than 20 mg·kg−1 copper diet was observed. In current study, with the increase in copper level from 20 to 300 mg·kg−1 in diet, the correlation between hair Na, K and Cu, Mn, Zn vanish. Hair Na and K were positively correlated with serum total antioxidant capacity (T-AOC) and negatively correlated with tumor necrosis factor-α (TNF-α). The hair Cu was negatively correlated with serum malondialdehyde (MDA), total bile acid (TBA). The fecal Cu was positively correlated with serum growth hormone (GH). The results suggested that the average daily gain (ADG) in 6 mg·kg−1 copper diet and the average daily feed intake (ADFI) in 20 mg·kg−1 copper diet were decreased than 300 mg·kg−1 copper diet during d 14 to 28 and the ADG was decreased in 6 and 20 mg·kg−1 copper diets in d 29 to 40 of piglets. Dietary 20 mg·kg−1 copper maintain ion homeostasis due to increase the number of positive correlations between macroelements-microelements in hair and serum. Significantly changed Na, K, Mg, Mn and Cu concentrations in hair can reflect the adverse effects of dietary 300 mg·kg−1 copper of suckling piglets. We believe our results may benefit people to gain a better understanding of the ion interactions and metabolic homeostasis of heavy metal elements that are critical to human and animal health.
Analysis of docosahexaenoic acid hydroperoxide isomers in mackerel using liquid chromatography–mass spectrometry
Authors: Kusumoto Ibuki , Kato Shunji , Nakagawa Kiyotaka 
Published: January 2023

AbstractDocosahexaenoic acid (DHA) is mostly esterified in food and is easily oxidized by exposure to heat or light. Hydroperoxide positions of DHA mono-hydroperoxide (DHA;OOH) provide information on oxidation mechanisms (e.g., radical- or singlet oxygen oxidation), yet direct identification of esterified DHA;OOH isomers has not been achieved. We previously accomplished the direct analysis of free DHA;OOH isomers with liquid chromatography-mass spectrometry (LC–MS/MS). In this study, we developed an LC–MS/MS method for direct analysis of esterified DHA;OOH based on our previous study. The developed method was capable of distinguishing esterified DHA;OOH isomers in raw- and oxidized mackerel. The result suggested that radical oxidation of esterified DHA can progress even in refrigeration. Different transitions were observed depending on the oxidation mechanism and lipid class. The analytical method and insights obtained in this study would be valuable to further understand and effectively prevent DHA oxidation in food products.
Seismic performance of steel-reinforced concrete-filled rectangular steel tubes after exposure to non-uniform fire
Authors: Han Yi , Bao Yan-Hong 
Published: January 2023

AbstractIn actual engineering, non-uniform fire boundary circumstances including single-sided fire, neighboring or related two-sided fire, and three-sided fire, are created due to the varying placements of the columns. In this paper, the seismic performance of SRCFST members subjected to non-uniform fire was investigated by the method of finite element simulation. First of all, the P-Δ curve, ductility coefficient, stiffness, and energy dissipation of the members following non-uniform fire were investigated. As the number of fire surfaces decreases, the maximum overfire temperature at the center of the section decreases, damage decreases, stiffness degradation decreases, and energy dissipation capacity increases. Next, the load distribution of each component in the SRCFST member was calculated using a three-sided fire as an example, the results show that steel tubes play the most dominant role in the seismic performance after fire, followed by steel sections and concrete the least. Last, a parametric study of the key variables influencing the ductility coefficient was carried out.
Computational fluid dynamics comparison of prevalent liquid absorbents for the separation of SO2 acidic pollutant inside a membrane contactor
Authors: Cao Yan , Taghvaie Nakhjiri Ali , Ghadiri Mahdi 
Published: January 2023

AbstractIn recent years, the emission of detrimental acidic pollutants to the atmosphere has raised the concerns of scientists. Sulphur dioxide (SO2) is a harmful greenhouse gas, which its abnormal release to the atmosphere may cause far-ranging environmental and health effects like acid rain and respiratory problems. Therefore, finding promising techniques to alleviate the emission of this greenhouse gas may be of great urgency towards environmental protection. This paper aims to evaluate the potential of three novel absorbents (seawater (H2O), dimethyl aniline (DMA) and sodium hydroxide (NaOH) to separate SO2 acidic pollutant from SO2/air gaseous stream inside the hollow fiber membrane contactor (HFMC). To reach this goal, a CFD-based simulation was developed to predict the results. Also, a mathematical model was applied to theoretically evaluate the transport equations in different compartments of contactor. Comparison of the results has implied seawater is the most efficient liquid absorbent for separating SO2. After seawater, NaOH and DMA are placed at the second and third rank (99.36% separation using seawater > 62% separation using NaOH > 55% separation using DMA). Additionally, the influence of operational parameters (i.e., gas and liquid flow rates) and also membrane/module parameters (i.e., length of membrane module, hollow fibers’ number and porosity) on the SO2 separation percentage is investigated as another highlight of this paper.