bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroMundo: experimental project fostering contribution to knowledge on antimicrobial resistance in secondary school
Authors: Robredo Beatriz , Fernández-Fernández Rosa , Torres Carmen , Ladrera Rubén 
Published: February 2023

AbstractAntimicrobial resistance (AMR) has become a serious global health threat. Education could play a decisive role, so the scope of innovative educational projects, such as MicroMundo, should be analysed. MicroMundo is a service-learning project for the exploration of microbial biodiversity in soils in search of new antibiotics. In order to evaluate the contribution of MicroMundo to the improvement of knowledge about AMR as well as the optimal age of the participants, an open questionnaire before and after the educational intervention (for secondary school students) and a satisfaction survey (for all participants) were carried out. Programme instruction began in university and continued through secondary school; 14 teachers (phase 1) and 137 students from three educational levels (phase 2) participated. MicroMundo was successfully implemented, showing a statistically significant improvement in knowledge about antibiotics, resistance, health and environmental consequences, and possible measures to reverse the problem, at all educational levels. Furthermore, the satisfaction surveys revealed a growing interest in research and science. Therefore, students of general branches who have not yet opted for a scientific line would be the most suitable to conduct the programme. In conclusion, MicroMundo is recommended as a training method to raise awareness about AMR and promote scientific vocations.
The Caenorhabditis elegans innexin INX-20 regulates nociceptive behavioral sensitivity
Authors: Chaubey Aditi H , Sojka Savannah E , Onukwufor John O , Ezak Meredith J , Vandermeulen Matthew D , Bowitch Alexander , Vodičková Anežka , Wojtovich Andrew P , Ferkey Denise M 
Published: February 2023

Abstract
Organisms rely on chemical cues in their environment to indicate the presence or absence of food, reproductive partners, predators, or other harmful stimuli. In the nematode Caenorhabditis elegans, the bilaterally symmetric pair of ASH sensory neurons serves as the primary nociceptors. ASH activation by aversive stimuli leads to backward locomotion and stimulus avoidance. We previously reported a role for guanylyl cyclases in dampening nociceptive sensitivity that requires an innexin-based gap junction network to pass cGMP between neurons. Here, we report that animals lacking function of the gap junction component INX-20 are hypersensitive in their behavioral response to both soluble and volatile chemical stimuli that signal through G protein-coupled receptor pathways in ASH. We find that expressing inx-20 in the ADL and AFD sensory neurons is sufficient to dampen ASH sensitivity, which is supported by new expression analysis of endogenous INX-20 tagged with mCherry via the CRISPR-Cas9 system. Although ADL does not form gap junctions directly with ASH, it does so via gap junctions with the interneuron RMG and the sensory neuron ASK. Ablating either ADL or RMG and ASK also resulted in nociceptive hypersensitivity, suggesting an important role for RMG/ASK downstream of ADL in the ASH modulatory circuit. This work adds to our growing understanding of the repertoire of ways by which ASH activity is regulated via its connectivity to other neurons and identifies a previously unknown role for ADL and RMG in the modulation of aversive behavior.
“They didn’t really have key experiences that they thought they could bring to the table”: Perceptions of white racial absolution during cross-racial intergroup dialogues
Authors: James-Gallaway Chaddrick D , Hudock Marigold M , Franklin Corbin 
Published: February 2023

AbstractThis article analyzes how White racial absolution, a form of White resistance to interrogating White racial identity through discourse, impedes cross-racial intergroup dialogs (IGDs) and impacts the IGD experience of Students of Color (SOC). Eleven undergraduate IGD students and six undergraduate IGD facilitators participated, and critical race discourse analysis (CRDA) was used for analysis. Findings showed that White students engaged in White racial absolution by avoiding White racial sharing (WRS), or side-stepping conversations regarding their Whiteness. The three themes around WRS included (a) Whiteness as a justification to not contribute to the IGD, (b) offering surface-level commentary regarding Whiteness, and (c) leaning on other social-identity-based marginalities or summoning the racial experiences of People of Color. Implications from this study illustrate that White racial absolution within cross-racial IGD alienates SOC, which stifles the overarching goal of resolving gaps between people of diverse social identity backgrounds.
Capacity of the Ethiopian primary health care system to achieve universal health coverage: a primary health care progression approach
Authors: Yitbarek Kiddus , Serawit Absalat , Medhin Girmaye , Alemayehu Yibeltal Kiflie , Teklu Alula M , Assefa Yibeltal , Haregu Tilahun Nigatu 
Published: February 2023

AbstractComprehensive and globally comparable evidence about primary health care (PHC) capacity is needed to inform policies and decisions. We carried out a study to assess the Ethiopian PHC capacity in terms of governance, inputs, and population health and facility management domains. The PHC capacity of all the regions, city administrations and the Ministry of Health was assessed using the PHC progression model. The model has 33 measures categorized into three domains. Data were collected and synthesized from all relevant national and regional documents, datasets and key informants. A team of trained evaluation experts conducted external assessments at national and regional levels followed by an internal assessment and a validation workshop. All 33 measures were scored from 1 (lowest) to 4 (highest). The inter-rater reliability test indicated that the overall agreement between internal and external scores was 65%. We found the highest consistency in the internal assessment with a score of 0.84. The findings of this study indicated that the governance domain score was 2.8 out of 4, showing varying scores in quality management, priority setting as well as innovation and learning. The inputs domain score was 2.3 for drugs, supplies and facility infrastructure. The score for the population health and facility management domain was 2. A comparison of federal and national average scores for all measures indicated no significant difference between the two (P-value = 0.69). There are relevant PHC policies and leadership structures at the federal and regional levels. However, the capacity to effectively implement these policies and strategies at sub-national levels is sub-optimal. The challenges related to major inputs coupled with data-quality problems reduced the capacity of the PHC system at the local level. Periodic assessment of the PHC system and closely working with subnational units will potentially improve the capacity of PHC in Ethiopia.
Potentiation of neuromuscular transmission by a small molecule calcium channel gating modifier improves motor function in a severe spinal muscular atrophy mouse model
Authors: Ojala Kristine S , Kaufhold Cassandra J , Davey Mykenzie R , Yang Donggyun , Liang Mary , Wipf Peter , Badawi Yomna , Meriney Stephen D 
Published: February 2023

Abstract
Spinal muscular atrophy (SMA) is a monogenic disease that clinically manifests as severe muscle weakness owing to neurotransmission defects and motoneuron degeneration. Individuals affected by SMA experience neuromuscular weakness that impacts functional activities of daily living. We have used a mouse model of severe SMA (SMNΔ7) to test whether a calcium channel gating modifier (GV-58), alone or in combination with a potassium channel antagonist (3,4-diaminopyridine; 3,4-DAP), can improve neuromuscular function in this mouse model. Bath application of GV-58 alone or in combination with 3,4-DAP significantly restored neuromuscular transmission to control levels in both a mildly vulnerable forearm muscle and a strongly vulnerable trunk muscle in SMNΔ7 mice at postnatal days 10–12. Similarly, acute subcutaneous administration of GV-58 to postnatal day 10 SMNΔ7 mice, alone or in combination with 3,4-DAP, significantly increased a behavioral measure of muscle strength. These data suggest that GV-58 may be a promising treatment candidate that could address deficits in neuromuscular function and strength and that the addition of 3,4-DAP to GV-58 treatment could aid in restoring function in SMA.
Antifungal activity and metabolomics analysis ofPiper sarmentosumextracts againstFusarium graminearum
Authors: Zhou Luli , Zhou Hanlin , Hou Guanyu , Ji Fengjie , Wang Dingfa 
Published: February 2023

AbstractAimsFusarium graminearum is a toxic fungus that affects food and feed crops. Piper sarmentosum extract (PSE) is a potential source of anti-mildew natural products for the food and feed industry due to its various pharmacological properties. In this study, we evaluated the antifungal activity and untargeted metabolomics analysis of PSE against F. graminearum.Methods and resultsAntifungal activity was evaluated using the mycelium growth rate method. Untargeted metabolomics analysis of PSE was performed using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that PSE (1 and 2 mg ml-1) possesses inhibitory activity against F. graminearum, and a total of 17 compounds that including 8 alkaloids, 3 phenols, 3 lipids, and 3 organic acids might be the antifungal markers in PSE. Metabolomics analysis further revealed that PSE could significantly increase the levels of guanosine, guanine, adenosine, and L-isoleucine in fungi, which are related to purine and L-isoleucine metabolic pathways.ConclusionsPSE is a promising anti-mildew agent that inhibits the growth of F. graminearum in food and feed. PSE (1 and 2 mg ml-1) may exert antifungal properties by inhibiting fungal purine nucleotide synthesis and enhancing the level of L-isoleucine compared with the control groups.
Characterizing the sarcoplasmic proteome of aged pork chops classified by purge loss
Authors: Johnson Logan G , Zhai Chaoyu , Reever Leah M , Prusa Kenneth J , Nair Mahesh N , Huff-Lonergan Elisabeth , Lonergan Steven M 
Published: February 2023

AbstractUnpredictable variation in quality, including fresh pork water-holding capacity, remains challenging to pork processors and customers. Defining the diverse factors that influence fresh pork water-holding capacity is necessary to make progress in refining pork quality prediction methods. The objective was to utilize liquid chromatography and mass spectrometry coupled with tandem mass tag (TMT) multiplexing to evaluate the sarcoplasmic proteome of aged pork loins classified by purge loss. Fresh commercial pork loins were collected, aged 12 or 14 d postmortem, and pork quality and sensory attributes were evaluated. Chops were classified into Low (N = 27, average purge = 0.33%), Intermediate (N = 27, average purge = 0.72%), or High (N = 27, average purge = 1.19%) chop purge groups. Proteins soluble in a low-ionic strength buffer were extracted, digested with trypsin, labeled with 11-plex isobaric TMT reagents, and detected using a Q-Exactive Mass Spectrometer. Between the Low and High purge groups, 40 proteins were differentially (P &lt; 0.05) abundant. The Low purge group had a greater abundance of proteins classified as structural and contractile, sarcoplasmic reticulum and calcium regulating, chaperone, and citric acid cycle enzymes than the High purge group. The presence of myofibrillar proteins in the aged sarcoplasmic proteome is likely due to postmortem degradation. These observations support our hypothesis that pork chops with low purge have a greater abundance of structural proteins in the soluble protein fraction. Together, these and other proteins in the aged sarcoplasmic proteome may be biomarkers of pork water-holding capacity. Additional research should establish the utility of these proteins as biomarkers early postmortem and over subsequent aging periods.
Scar Management and Dyschromia: A Summary Report from the 2021 American Burn Association State of the Science Meeting
Authors: Carney Bonnie C , Bailey John K , Powell Heather M , Supp Dorothy M , Travis Taryn E 
Published: February 2023

Abstract
Burn scars, and in particular, hypertrophic scars, are a challenging yet common outcome for survivors of burn injuries. In 2021, the American Burn Association brought together experts in burn care and research to discuss critical topics related to burns, including burn scars, at its State of the Science conference. Clinicians and researchers with burn scar expertise, as well as burn patients, industry representatives, and other interested stakeholders met to discuss issues related to burn scars and discuss priorities for future burn scar research. The various preventative strategies and treatment modalities currently utilized for burn scars were discussed, including relatively noninvasive therapies such as massage, compression, and silicone sheeting, as well as medical interventions such as corticosteroid injection and laser therapies. A common theme that emerged is that the efficacy of current therapies for specific patient populations is not clear, and further research is needed to improve upon these treatments and develop more effective strategies to suppress scar formation. This will necessitate quantitative analyses of outcomes and would benefit from creation of scar biobanks and shared data resources. In addition, outcomes of importance to patients, such as scar dyschromia, must be given greater attention by clinicians and researchers to improve overall quality of life in burn survivors. Herein we summarize the main topics of discussion from this meeting and offer recommendations for areas where further research and development are needed.
Determination of Residual Control and Concentration of Chlorantraniliprole in Soybean
Authors: Smith Jacob , Crow W D , Catchot A L , Cook D , Gore J 
Published: February 2023

Abstract
Studies were conducted in 2020 and 2021 at the Delta Research and Extension Center in Stoneville, MS to determine concentrations of chlorantraniliprole (Prevathon, FMC Corporation, Philadelphia, PA) in soybean (Glycine max L.) leaves and florets. Chlorantraniliprole was applied as a foliar spray at four rates (0.028, 0.053, 0.078, 0.103 kg ai ha−1) for leaves and two rates (0.053, 0.078 kg ai ha−1) for florets. Leaf bioassays with corn earworm, Helicoverpa zea (Boddie), were conducted concurrently to determine mortality within three plant zones to evaluate chlorantraniliprole distribution throughout the canopy. For the leaf study, plants were partitioned into three zones consisting of a top (18th node), middle (13th node), and bottom (9th node) zone. Leaf samples from each zone were analyzed for chemical concentrations and bioassays were conducted at 1, 7, 14, 21, and 28 days after treatment (DAT). Floret samples were analyzed at 4, 7, 10, and 14 DAT. Concentrations of chlorantraniliprole, though variable, provided &gt;71% control through all sampling dates, application rates, and canopy zones tested. Chlorantraniliprole was viable up to 28 DAT. Results from the soybean floret study suggested chlorantraniliprole was detected in florets up to 14 DAT. An additional leaf bioassay was conducted using concentrations detected in the floret study. Concentrations in florets provided mortality of corn earworm up to 48% out to 14 DAT. With a long residual expected, chlorantraniliprole applications should continue to be used to control corn earworm infestations in soybean and some additional control could be expected in florets.
Evolutionary potential mitigates extinction risk under climate change in the endangered southwestern willow flycatcher
Authors: Forester Brenna R , Day Casey C , Ruegg Kristen , Landguth Erin L 
Published: February 2023

AbstractThe complexity of global anthropogenic change makes forecasting species responses and planning effective conservation actions challenging. Additionally, important components of a species’ adaptive capacity, such as evolutionary potential, are often not included in quantitative risk assessments due to lack of data. While genomic proxies for evolutionary potential in at-risk species are increasingly available, they have not yet been included in extinction risk assessments at a species-wide scale. In this study, we used an individual-based, spatially explicit, dynamic eco-evolutionary simulation model to evaluate the extinction risk of an endangered desert songbird, the southwestern willow flycatcher (Empidonax traillii extimus), in response to climate change. Using data from long-term demographic and habitat studies in conjunction with genome-wide ecological genomics research, we parameterized simulations that include 418 sites across the breeding range, genomic data from 225 individuals, and climate change forecasts spanning 3 generalized circulation models and 3 emissions scenarios. We evaluated how evolutionary potential, and the lack of it, impacted population trajectories in response to climate change. We then investigated the compounding impact of drought and warming temperatures on extinction risk through the mechanism of increased nest failure. Finally, we evaluated how rapid action to reverse greenhouse gas emissions would influence population responses and species extinction risk. Our results illustrate the value of incorporating evolutionary, demographic, and dispersal processes in a spatially explicit framework to more comprehensively evaluate the extinction risk of threatened and endangered species and conservation actions to promote their recovery.
Genetic architectures of postmating isolation and morphology of two highly diverged rockfishes (genusSebastes)
Authors: Muto Nozomu , Kawasaki Takuma , Kakioka Ryo , Nagano Atsushi J , Shimizu Yuta , Inose Shu , Shimizu Yohei , Takahashi Hiroshi 
Published: February 2023

AbstractPostmating isolation is thought to be an important driver of the late stages of speciation. However, relatively little is empirically known about the process compared with other isolating mechanisms that drive the early stages of speciation, especially in non-model organisms. We characterized the genetic architecture of postmating isolation between 2 rockfishes, Sebastes schlegelii and S. trivittatus, whose reproductive isolation is complete. We examined transmission ratio distortion (TRD) patterns of genetic markers in 2 reciprocal backcross populations. Markers showing either of the 2 types of TRD was widespread across the genome, with some of the distorted markers forming extensive clusters around the recombination coldspots. These suggest that the postmating isolation effectively prevents gene flow across the genome and the recombination landscape contributes to the genetic architecture. Comparisons between 2 backcross families and 2 developmental stages showed little similarity in the distorted markers, suggesting asymmetry and stage specificity of the isolation. This may be due to hybrid incompatibility involving maternal factors or extrinsic selection. The lack of sex-ratio distortion in the mapping families suggested that Haldane’s rule in terms of hybrid inviability does not hold. Additionally, quantitative trait loci (QTL) mapping detected significant QTLs for sex and the morphological traits relevant to speciation and convergence of rockfishes, including body coloration. Genes in the melanocortin system, including agouti-signaling protein 1 (asip1) and melanocortin 1 receptor (mc1r), might underlie the horizontal and vertical color patterns on the body, respectively. These findings constitute an essential step toward a comprehensive understanding of speciation and morphological diversification of rockfishes.
Characterizing the Vector Data Ecosystem
Authors: Lippi Catherine A , Rund Samuel S C , Ryan Sadie J 
Published: February 2023

AbstractA growing body of information on vector-borne diseases has arisen as increasing research focus has been directed towards the need for anticipating risk, optimizing surveillance, and understanding the fundamental biology of vector-borne diseases to direct control and mitigation efforts. The scope and scale of this information, in the form of data, comprising database efforts, data storage, and serving approaches, means that it is distributed across many formats and data types. Data ranges from collections records to molecular characterization, geospatial data to interactions of vectors and traits, infection experiments to field trials. New initiatives arise, often spanning the effort traditionally siloed in specific research disciplines, and other efforts wane, perhaps in response to funding declines, different research directions, or lack of sustained interest. Thusly, the world of vector data – the Vector Data Ecosystem – can become unclear in scope, and the flows of data through these various efforts can become stymied by obsolescence, or simply by gaps in access and interoperability. As increasing attention is paid to creating FAIR (Findable Accessible Interoperable, and Reusable) data, simply characterizing what is ‘out there’, and how these existing data aggregation and collection efforts interact, or interoperate with each other, is a useful exercise. This study presents a snapshot of current vector data efforts, reporting on level of accessibility, and commenting on interoperability using an illustration to track a specimen through the data ecosystem to understand where it occurs for the database efforts anticipated to describe it (or parts of its extended specimen data).
Necrotising fasciitis: a case series set in rural Australia
Authors: Kay Tegan J , Wallace Lauren , Bennett Laura , Gallagher Peter J 
Published: February 2023

Abstract
Necrotising fasciitis (NF) is a rapidly progressive infection of soft tissue and fascia. Early diagnosis and prompt extensive surgical debridement decrease mortality. This remains a challenge for rural surgeons who have limited experience with the disease, in a setting where patient transfers to tertiary centres are lengthy and often delayed. To assist clinical decision making in this setting, a case series of five NF presentations in a rural Australian hospital were retrospectively analysed for presentation, investigation, treatment and clinical outcomes. Three underwent abdominal wall debridement and two underwent below knee amputation. Results demonstrate early recognition of NF and the extent of surgical intervention prior to acute transfer are key to successful outcomes. Expedient diagnosis and early extensive debridement at the initial contact reduce mortality and should be the goal of management in this setting.