bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blunt traumatic injury in a pediatric patient treated with simultaneous damage control laparotomy, thoracotomy and transcatheter arterial embolization
Authors: Tomonaga Ayumi , Nagao Tsuyoshi , Kono Kaori , Kanda Tomoki , Ito Kaori 
Published: June 2023

Abstract
Background: The hybrid emergency room system (HERS) is a single trauma resuscitation room with computed tomography (CT) scanner, fluoroscopy, operating room, and angiography suite allowing surgeons and interventional radiologists (IRs) to obtain imaging studies and perform simultaneous major surgery and endovascular procedures in one room. We report a pediatric patient who suffered severe blunt trauma who underwent simultaneous damage control laparotomy with thoracotomy and endovascular treatment in the HERS.
Case presentation: An 8-year-old male was struck and run over (chest and abdomen) by a vehicle. The patient was admitted to the HERS from the ambulance in hemodynamic shock. Focused Assessment of Sonography for Trauma revealed intraabdominal free fluid. Blood gas analysis showed lactic acidosis. The patient was intubated. The massive blood transfusion protocol was activated. Fluoroscopy confirmed no major injuries in the chest and pelvis and exploratory laparotomy was performed which revealed a liver injury (Abbreviated Injury Scale [AIS] 5) and splenic injury (AIS 2) with 1,000 ml of free intraabdominal blood. Perihepatic packing was performed. Simultaneously, IRs performed transcatheter arterial embolization of small branches of the right hepatic and subdiaphragmatic arteries. Subsequently, whole-body CT scan with intravenous contrast was performed which revealed a massive right hemopneumothorax with the extravasation of contrast medium from a right inferior pulmonary artery branch. Right anterolateral thoracotomy was immediately performed. A large laceration of the right lung S6 (AIS 4) was sutured (Injury Severity Score 42, Revised Trauma Score 1.9, TRISS Probability of survival 0.08). The chest and abdomen were temporarily closed, and the patient transferred to the ICU from the HERS. The right chest was closed on hospital day (HD) 2 and the abdomen closed on HD 3. The patient was extubated on HD 6. The postoperative course was complicated by. refractory right atelectasis. High-flow oxygen therapy and respiratory physiotherapy were continued. The patient was discharged home on HD26.
Conclusions: The HERS may be useful not only for adult trauma patients but also for severely injured pediatric trauma patients. Protocolized trauma care in the HERS to facilitate collaboration among multiple disciplines and specialties is essential.
Estrogen regulates Scribble localization in endometrial epithelial cells through acyl protein thioesterase (APT)-mediated S-palmitoylation in adenomyosis
Authors: jin zhixing , Wang Juan , Chen Youguo 
Published: June 2023

Abstract
Despite its prevalence and the severity of symptoms, little is known about the pathogenesis and etiology of adenomyosis. In our previous study, Scribble localization has been found to be partially translocated to cytoplasm, however, its regulatory mechanism is known. In consideration of the important role of supraphysiologic estrogen production in the endometrium in the development of adenomyosis, we analyzed the effect and mechanism of estrogen on Scribble localization in vivo and in vitro. Firstly, we found Scribble translocation from the basolateral membrane to the cytoplasm was easily to be seen in women and mice with adenomyosis (68% vs 27%, 60% vs 10% separately). After treatment with the S-palmitoylation inhibitor 2-Bromopalmitate for 48H, cytoplasmic enrichment of Scribble and the reduced level of palm-Scribble was observed by immunofluorescence, Western blot and acyl-biotin exchange palmitoylation assay. High estrogen exposure could not only induce partially cytoplasmic translocation of Scribble but also decrease the expression level of palm-Scribble, which can be recovered by estrogen receptor inhibitor ICI182,780. Based on following experiments, we found that estrogen regulated Scribble localization by APT through S-palmitoylation of Scribble protein. At last, IHC was performed to verify the expression of APT1 and APT2 in human clinical tissue specimens and found that they were all increased dramatically. Furthermore, positive correlations were found between APT1 or APT2 and aromatase P450. Therefore, our research may provide a new understanding of the pathogenesis of adenomyosis.
Synthesis of zeolites using aluminosilicate residues from the lithium extraction
Authors: Ibsaine Fatima , Azizi Dariush , Dionne Justine , Tran Lan Huong , Coudert Lucie , Pasquier Louis-César , Blais Jean-Francois 
Published: June 2023

Abstract
The production of lithium from spodumene ores generates huge amounts of residue mainly composed of aluminosilicate. The main objective of this study was to compare the performances of three different processes to produce zeolites from aluminosilicates residues originating from lithium extraction. Zeolites were synthesized using: i) a conventional hydrothermal process (Process_1), ii) a conventional hydrothermal process assisted by calcination (Process_2), and iii) a conventional hydrothermal process assisted by alkaline fusion (Process_3). A physico-chemical (e.g., chemical composition, sorption capacity) and mineralogical (e.g., XRD, SEM) characterization of synthesized and commercial zeolite was done to identify the most performing synthesis route. Then, the effect of operating parameters (i.e., aging time and temperature, crystallization time, solid/liquid ratio) on the physico-chemical properties of the zeolite synthesized using the most performant process route was assessed. Initial aluminosilicate residues were mainly composed of Al2O3 (24.6%) and SiO2 (74.0%), while containing low amounts of potential contaminants (< 1.6%). Based on its chemical composition, the fine fraction (< 53 µm) was identified as the most suitable fraction to produce zeolite. Physico-chemical and mineralogical characterization of produced zeolite showed that conventional hydrothermal process was the most performant route to synthesize zeolite with properties like commercial zeolite 13X. Crystallization time (from 8 to 24 h), aging temperature (from 25 to 75°C) and S/L ratio (from 10 to 30% - w/v) are the main parameters affecting the properties of synthesized zeolite (i.e., ion-exchange capacity). Finally, a zeolite type X with an ion-exchange capacity of 58 mg/g, which is close to commercial zeolites (76–77 mg/g), was synthesized from the fine fraction of aluminosilicate residue using the conventional hydrothermal process after 8 h of aging at 75°C and 16 h of crystallization at 100°C, with a solid/liquid ratio of 10% (w/v).
Spatial Distribution of Wildfire Threat in the Far North: Exposure Assessment in Boreal Communities
Authors: Schmidt Jennifer , Ziel Robert , Calef Monika , Varvak Anna 
Published: June 2023

Abstract
Increased wildfire activity has raised concerns among communities about how to assess and prepare for this threat. We modified an existing approach to assess decadal wildfire hazards based primarily on ember dispersal and wildfire proximity, referencing landscape changes from 1984 through 2014. The original method created multiple maps to capture ember dispersal and spread at different scales. Instead, we integrated this multi-scale information into a single exposure layer and utilized a straightforward flammability hazard classification scheme. Binomial exact and Kruskal–Wallis tested the relationship between exposure values, underlying flammability hazard classes, and wildfire scars, respectively, in three arctic communities (Anchorage and Fairbanks, Alaska and Whitehorse, Yukon) with a range of wildfire histories and amounts of urbanization. There was a significant difference in exposure values among burned and unburned locations (p < 0.001) and flammability hazard classes (p < 0.001). Areas with high exposure values are more prone to burn and thus desirable for mitigation actions. Wildfire fire hazards are extremely high within Whitehorse and Fairbanks, and lower in Anchorage. By working with wildfire practitioners, communities, and residents, we have created a tool that can rapidly assess wildfire hazards and be easily modified to help identify and prioritize areas for mitigation activities.
Valorization of noni (Morinda citrifolia) seeds as source of a protein concentrate and its physicochemical, funcional and structural characterization
Authors: Ramírez Jorge Adrían Hernández , Ulloa José Armando , Rangel Blanca Estela Ulloa , Ulloa Petra Rosas 
Published: June 2023

Abstract
The noni juice industry produces a large volume of seeds as by-products, which contain proteins that can be recovered to be used as food ingredients. The aims of this study were the valorization of noni seeds as source of a protein concentrate (NSPC) and their physicochemical, functional, and structural characterization. NSPC was produced by alkaline extraction and isoelectric precipitation, obtaining a powder with a protein content of 76.59%, whose values of the color parameters were L* = 55. 32%, a* = 4.60%, and b* = 19.09. Water (WHCa) and oil (OHCa) holding capacities of the NSPC were 4.36 g H2O/g protein and 11.69 g oil/g protein, respectively, while the emulsifying activity, emulsion stability, foaming capacity, foaming stability, least gelling concentration, and in vitro digestibility were, 29.20%, 50.00%, 180.33%, 94.60%, 4.00%, and 78.51%. Glutelins (64.62%) were the main protein fraction of the NSPC. Hence, NSPC could be used as ingredient for human food products as bread, soups, salad dressings, mayonnaise, and processed meat products.
CAR T-cell design dependent remodeling of the brain tumor immune microenvironment identify macrophages as key players that inhibit or promote anti-tumor activity
Authors: Haydar Dalia , Ibañez-Vega Jorge , Crawford Jeremy , Chou Ching-Heng , Guy Cliff , Meehl Michaela , Yi Zhongzhen , Langfitt Deanna , Vogel Peter , DeRenzo Christopher , Gottschalk Stephen , Roussel Martine , Thomas Paul , Krenciute Giedre 
Published: June 2023

Abstract
Understanding interactions between adoptively transferred immune cells and the tumor immune microenvironment (TIME) is critical for developing successful T-cell based immunotherapies. Here we investigated the impact of the TIME and chimeric antigen receptor (CAR) design on anti-glioma activity of B7-H3-specific CAR T-cells. We show that five out of six B7-H3 CARs with varying transmembrane, co-stimulatory, and activation domains, exhibit robust functionality in vitro. However, in an immunocompetent glioma model, these CAR T-cells demonstrated significantly varied levels of anti-tumor activity. We used single-cell RNA sequencing to examine the brain TIME after CAR T-cell therapy. We show that the TIME composition was influenced by CAR T-cell treatment. We also found that successful anti-tumor responses were supported by the presence and activity of macrophages and endogenous T-cells. Together, our study demonstrates that efficacy of CAR T-cell therapy in high-grade glioma is dependent on CAR structural design and its capacity to modulate the TIME.
Fostering rice intensification to reduce imports and land conversion in Africa
Authors: Saito Kazuki , Oort Pepijn van , Ittersum Martin van , Peng Shaobing , Yuan Shen , Grassini Patricio 
Published: June 2023

Abstract

Africa produces 40% of its rice consumption, relying heavily on rice imports to fulfill the rest of the domestic demand. Over the past 10 years, rice area increased nearly 40%, while average yield remained stagnant. Here we evaluate the room that exists to increase rice production on existing cropland area in Africa and assess cropland expansion and rice imports by year 2050 for different scenarios of yield intensification. We find that Africa can avoid further increases in rice imports, and even reduce them, through a combination of cropland expansion following the historical trend together with closure of the current exploitable yield gap by half or more. Without substantial increase in rice yields, meeting future rice demand will require larger rice imports and/or land conversion than now.
Analysis of Flow Field Characteristics of Sand Removal Hydroyclone Applicable to Solid Fluidization Exploitation of Natural Gas Hydrate
Authors: Wei Na , Qiao Yi , Fan Shuanshi , Cai Meng , Li Haitao , Zhou Shouwei , Zhao Jinzhou , Zhang Liehui , Coffin Richard 
Published: June 2023

Abstract
With the development of economy and society, the consumption of fossil energy is gradually increasing. In order to solve the current energy dilemma, NGH is considered as an ideal alternative energy. At the same time, the solid fluidization exploitation is an ideal exploit method for NGH at present. However, in the process of solid fluidization exploitation, sand and hydrate ore bodies enter the closed pipeline together, which will block the pipeline and increase the difficulty of exploitation. Therefore, the pre-separation of sand by hydrocyclone plays an important role in solid fluidization exploitation. In this study, the numerical simulation method was used to study the internal flow field characteristics of the hydrocyclone under different conditions, and the effects of different flow rate, different flow ratio, different sand content and different particle diameter on the phase distribution are investigated. The results show that: at the same axial position, the increase of flow rate and sand content makes the sand phase more distributed at the edge of the flow field, while the change of flow ratio has no significant effect on the distribution of sand phase. Under the same working conditions, the sand gradually migrates to the center of the flow field with the increase of the axial distance, while the particle diameter change has no significant effect on the sand distribution. By calculation, it is obtained that under the optimum working condition of the flow rate is 4.83m3/h, the flow ratio is 20%, the sand content is 20%, and sand particle diameter is 80µm, the maximum Es is 22.1% and the minimum is 86.1%. Finally, a comprehensive analysis of the hydrocyclone in this study shows that this type of hydrocyclone is applicable to rough pre-separation of sand in the process of solid fluidization exploitation of NGH, and can not fine separate complex mixture. Through the study of the internal flow field characteristics and phase distribution law of the hydrocyclone, this study provides a reference for the practical engineering application of sand phase pre-separation in the solid fluidization exploitation of NGH.
First-order control of basaltic sources on the diversity of Archean TTG magmatism
Authors: Lu Denggang , Liu Shuwen , Cawood Peter , Gao Lei , Sun Guozheng , Yao Jiachen , He Xin , Wang Wei 
Published: June 2023

Abstract

Tonalite-trondhjemite-granodiorite (TTG) gneisses, the major components of Archean continental crust, show a broad compositional range. Most were viewed as crust-derived, and the inferred melting P-T conditions shaped our knowledge about the origin of early continental crust. While one single basaltic source (e.g., median of Archean tholeiites) was commonly invoked for different TTG gneisses, the potential influences of wide-ranging basaltic sources remain not well constrained. Late Neoarchean tonalitic to trondhjemitic gneisses, covering the compositional spectrum of global database, were identified from the Jiaobei terrane, North China Craton. Thermodynamic and trace element modeling demonstrates that (1) their chemical diversity is primarily determined by different basaltic sources with variable TiO2 and heavy rare earth element (HREE) contents; and (2) the inferred melting pressures of most tonalitic to trondhjemitic gneisses would reduce dramatically if a different basaltic source with slightly lower TiO2 and HREE contents was assumed. Integrated with synchronous chemical changes of global Archean TTG and basaltic rocks, a source-based twofold classification of TTG gneisses (i.e., low- and high-Ti-HREE types) is put forward. The low-Ti-HREE TTG gneisses necessitate both low-Ti-HREE basaltic sources and high melting pressures (~1.8 GPa), and their outburst after ~3.2 Ga may mark the development of thickened continental crust globally.
Construction of CD8+ T cell-associated risk signature in hepatocellular carcinoma based on single-cell RNA-seq and bulk RNA-seq data
Authors: Zhang Xintong , Zhu Jianjun , Wu Jin , Wu Hao , Lu Fan , Zhang Wentao , Chang Jingjia , Tang Ting , Ou Zhigao , Jia Fengfeng , Li Li , Yu Pengfei , Liu Ming 
Published: June 2023

Abstract
Background
Hepatocellular carcinoma (HCC) is the most prevalent form of primary liver cancer, characterized by high recurrence and mortality rates. Specific CD8+ T cells have a central role in pathogenesis of HCC by recognizing and destroying tumor cells.
Method
Common CD8 + T cell signatures were obtained from bulk RNA-seq and scRNA-seq analysis. Univariate Cox regression, LASSO regression, and Multivariate Cox regression was sequentially utilized on the CD8 + T cell signatures to establish a risk scoring model. Prognostic value of the model was verified in the external datasets GSE14520. Immune analysis and Drug Sensitivity analysis was performed in high risk and low risk group. Immunohistochemistry (IHC) was employed to ascertain the expression of model genes in 10 HCC samples and corresponding adjacent tissues.
Result
A three-gene risk scoring model (KLRB1, RGS2 and TNFRSF1B) was constructed from CD8+ T cell common signatures. In terms of immune cell infiltration, low-risk group demonstrated higher abundance of CD8+ T cells, and M1 Macrophages., whereas high-risk group demonstrated higher abundance of M2 Macrophages and resting mast cells. As for immunotherapy, patients in high-risk group may not respond well. BMS-754807, Gemcitabine, et al exhibited sensitive to patients in low-risk group, AZD6482 and SB505124 may serve as novel potential targeted drugs for patients in high-risk group. In comparison to normal tissues, KLRB1, RGS2, and TNFRSF1B exhibited a reduced expression level, confirming our findings in publicly available databases.
Conclusion
In conclusion, we constructed a CD8+ T cell-associated risk scoring model consisting of KLRB1, RGS2 and TNFRSF1B to predict prognosis and provide potential drugs for HCC treatment.
Protective effect of gomisin N on BBP-induced dysfunction of testosterone production in TM3 Leydig cells
Authors: Lee Seung Ju , Park Min Ju , Jeong Dong Hyeok , Arunachalam Rohith , Yoo Eunsoo , Kim Hye Kyung , Park Min Hi 
Published: June 2023

Abstract
Background
Exposure to benzyl butyl phthalate (BBP) may induce disorders in the male reproductive system. However, the molecular mechanisms remain unknown. Here we investigated the effect of BBP on testosterone production and its molecular mechanisms. Furthermore, we also investigated the role of gomisin N (GN) from Schisandra chinensis (S. chinensis) in testosterone synthesis in TM3 Leydig cells.
Method and results
First, we examined the effects of BBP on expression levels of testosterone biosynthesis-related genes (StAR, CYP11α1, CYP17α1, 3βHSD, and 17βHSD) and attenuation-related genes (CYP1β1, CYP19α1, and Srd5α1-3). Although testosterone biosynthesis-related genes did not change, attenuation-related genes such as CYP1β1 and CYP19α1 were upregulated with ROS generation and testosterone level attenuation in the presence of 50 μM of BBP. However, the compound with the highest ROS and ONOO- scavenging activity from S. chinensis, GN, significantly reversed the expression of BBP-induced testosterone attenuation-related gene to normal levels. Subsequently, GN improved the testosterone production levels in TM3 Leydig cells. These events may be regulated by the antioxidant effect of GN.
Conclusions
In conclusion, our study suggests, for the first time, that BBP impairs testosterone synthesis by the modulation of CYP1β1 and CYP19α1 expression in TM3 cells; GN could potentially minimize the BBP-induced dysfunction of TM3 cells to produce testosterone by suppressing CYP19α1 expression.
PRFect: A tool to predict programmed ribosomal frameshifts in prokaryotic and viral genomes
Authors: McNair Katelyn , Salamon Peter , Edwards Robert A. , Segall Anca M. 
Published: June 2023

Abstract
Background
One of the stranger phenomena that can occur during gene translation is where, as a ribosome reads along the mRNA, various cellular and molecular properties contribute to stalling the ribosome on a slippery sequence, shifting the ribosome into one of the other two alternate reading frames. The alternate frame has different codons, so different amino acids are added to the peptide chain, but more importantly, the original stop codon is no longer in-frame, so the ribosome can bypass the stop codon and continue to translate the codons past it. This produces a longer version of the protein, a fusion of the original in-frame amino acids, followed by all the alternate frame amino acids. There is currently no automated software to predict the occurrence of these programmed ribosomal frameshifts (PRF), and they are currently only identified by manual curation.
Results
Here we present PRFect, an innovative machine-learning method for the detection and prediction of PRFs in coding genes of various types. PRFect combines advanced machine learning techniques with the integration of multiple complex cellular properties, such as secondary structure, codon usage, ribosomal binding site interference, direction, and slippery site motif. Calculating and incorporating these diverse properties posed significant challenges, but through extensive research and development, we have achieved a user-friendly approach. The code for PRFect is freely available, open-source, and can be easily installed via a single command in the terminal. Our comprehensive evaluations on diverse organisms, including bacteria, archaea, and phages, demonstrate PRFect's strong performance, achieving high sensitivity, specificity, and an accuracy exceeding 90%.
Conclusion
PRFect represents a significant advancement in the field of PRF detection and prediction, offering a powerful tool for researchers and scientists to unravel the intricacies of programmed ribosomal frameshifting in coding genes.