bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Nano Heavy Metal Oxide Particles on the Wettability of Carbonate Reservoir Rock
Authors: Pashaei Hassan , Ghaemi Ahad , Miri Rohaladin 
Published: April 2023

Summary
Production of oil from carbonate rocks is very challenging due to their inherent nature, such as detection, complex wettability, pore structure, and low recovery factor. Nanoparticles (NPs) are recognized as remarkable materials for a wide range of research and commercial applications due to their physical properties and characteristics. Extensive research in recent years has shown that nanoscience can provide great potential for the development of carbonate reservoirs and enhanced oil recovery (EOR). In this study, the carbonate core plug samples were prepared from an Iranian reservoir. At first, the wettability capacity of the core samples was evaluated. This process was carried out by evaluating wettability changes using the contact angle of base fluid and nanofluid. The potential of the NPs (ZnO, TiO2, and ZrO2) to change the wettability was experimentally tested in the loading NPs from 0.01 wt% to 0.5 wt% by the contact angle method. Wettability studies have shown that nanofluids can influence wettability variability from oil-wet to water-wet quality. About 0.05 wt% of NPs was found to be the optimal concentration to affect wettability change. The same behavior was observed for all nanofluids at the same NP loading; while TiO2 showed better performance with a sharp change from an oil-wet state (θ = 151.9°) to a water-wet state (θ = 111.3°), ZnO, and ZrO2 changed wettability to a moderately-wet condition (θ = 108.6° and 118.6°, respectively) at 0.05 wt% NP loading. We conclude that TiO2-based nanofluids have great potential as EOR agents, and TiO2 is very impressive in its strong water-wettability. The highest oil recovery in the optimal amount for all three nanofluids was obtained as 35.2%, 23.2%, and 25.6%, respectively, for TiO2, ZnO, and ZrO2 nanofluids. Furthermore, we considered the effect of nanofluids on the recovery performance of the brine/oil system for carbonate core samples. The results showed that nanofluids can significantly imbibe into the core sample, and as a result, the final oil recovery is significant.
Enhanced ethanol production using hydrophobic resin detoxified two-step pre-treated Pine forest litter hydrolysate, mass balance equation generation and BOLT-ON process development supplementing molasses
Authors: Pandey Ajay Kumar , Negi Sangeeta 
Published: April 2023

Abstract
High ethanol demand to make up the targets of the future gradual replacement of gasoline it is essential to look for hybrid technologies using first generation and second-generation biofuel feedstock’s. The current study used a two-step sequential pre-treatment (first dilute alkali, then dilute acid) of Pine (Pinus roxburghii) forest litter (PFL), for bioethanol production. Further, the saccharification of pre-treated PFL was optimized through response surface method using Box–Behnken Design, wherein 0.558 g/g of reducing sugar was under optimized conditions: 12.5% (w/v) of biomass loading, 10 FPU of enzyme, 0.15% (w/v) of Tween-80 in 48 h. During fermentation using Saccharomyces cerevisiae NCIM 3288 strain, the ethanol titre was 22.51 g/L, which was enhanced to 27.38 g/L after detoxification of PFL hydrolysate with hydrophobic resin (XAD-4). Furthermore, the hydrolysate was supplemented with molasses (total initial sugar: 100 g/L), wherein 46.02 ± 2.08 g/L ethanol was produced with 0.482 g/g yield and 1.92 g/l/h productivity. These results, showed that BOLT-ON Technology involving integration of molasses, increases the initial sugar availability leading to enhanced ethanol production, therefore can be compatible in bio-refineries to produce high titer ethanol.
Synthesis of Cu and CuO Nanoparticles from E-waste and Evaluation of Their Antibacterial and Photocatalytic Properties
Authors: Abdelbasir Sabah M. , Rayan Diaa A. , Ismail Mahmoud M. 
Published: April 2023

Abstract
Waste printed circuit boards (WPCBs) contain a plethora of valuable metals, considered an attractive secondary resource. In the current research, a hydrometallurgical process combined ammonia/ ammonium chloride leaching and reduction to recover copper and its oxide (CuO) as nanosized particles from WPCBs was investigated. The results of leaching indicated that 96.7% of copper could be recovered at a temperature of 35 °C for a leaching duration of 2 h with ammonium chloride and ammonia concentration of 2 mol/L at a solid:liquid ratio of 1:10 g/cm3. The synthesized particles exhibit spherical and distorted sphere morphology with average particle size of 460 nm and 50 nm for Cu and CuO NPs, respectively. The antibacterial activity of Cu, CuO, and a (1:1) blend of both (Cu/CuO) has been examined against five different bacterial and fungal strains. The highest zone of inhibition was measured as 21.2 mm for Cu NPs toward E. coli and 16.7 mm for Cu/CuO blend toward B. cereus bacteria. The highest zone of inhibition was measured as 13 mm and 13.8 mm for Cu/CuO blend toward F. proliferatum and P. verrucosum fungi. Cu/CuO blend showed notable photocatalytic activity towards Rhodamine B dye under visible light irradiation with 96% degradation rate within 120 minutes.
METTL3-mediated m6A methylation regulates ovarian cancer progression by recruiting myeloid-derived suppressor cells
Authors: Wang Jinyong , Ling Dakai , Shi Lulin , Li Huayun , Peng Minhua , Wen Huihong , Liu  , Zhang Guangzhi , chen shanze 
Published: April 2023

Abstract
Background
Ovarian cancer typically develops an immunosuppressive microenvironment with excess recruitment of various leukocytes. The infiltrated leukocytes often exhibit protumor or anti-tumor activity depending on the surrounding signals in the microenvironment. Dysregulated m6A level is associated with cancer progression by influencing intrinsic oncogenic pathways in tumor cells. However, the role of m6A in regulating host immune cell function during anti-tumor immunity still needs to be fully investigated.
Methods
We firstly generated the myeloid-specific Mettl3 gene knockout mice by using the CreLoxP system in which Mettl3fl/fl mice were crossed with Lyz2-Cre +/+ mice. ID8 were injected intraperitoneally into WT and Mettl3-cKO mice used as a syngeneic mouse model for evaluation of epithelial OC growth. Next, we analyzed the compositions of different immune cell populations in the TME by flow cytometry and single cell sequenencing. Furthurmore, we investigate why Mettl3-cKO mice recruit more Gr-1+ MDSCs in the peritoneal cavity after OC cells implantation by ELISA based screening of chemokines and cytokines in peritoneal lavage and serum. Lastly, we specify the role of METTL3 in regulating IL-1β secretion and inflammasome activation in Bone marrow-derived macrophages (BMDMs) cocultured with either ID8 cell by using immunoblotting and ELISA.
Results
In this study, we aimed to investigate the role of METTL3, a catalytic subunit of the methyltransferase complex, in regulating host immune cell response against ovarian cancer. We found that ovarian cancer cell growth was enhanced in Mettl3-deficient mice. A shift of decreased M1 polarization to increased M2 polarization of macrophages during ovarian cancer progression was observed. Furthermore, Mettl3 depletion in myeloid lineage cells facilitates the recruitment of Gr-1 + MDSCs by increased secretion of CCL2 and CXCL2. Interestingly, we demonstrated that Mettl3 deficiency enhances IL-1β secretion induced by viable ID8 cells but not cell lysates independent of inflammasome activation and cell death. Therefore, in tumor-bearing mice, ovarian cancer cells trigger a slight inflammatory response with a low-to-moderate secretion of pro-inflammatory cytokines and chemokines.
Conclusion
This study provides new insights into METTL3-mediated m6A methylation in regulating host immune response against ovarian cancer.
A prospective small-sample random controlled trial of navigation system-assisted distraction osteogenesis for hemifacial microsomia based on artificial intelligence
Authors: Liu Xiangqi , Zhang Ziwei , Han Wenqing , Zhao Zhijie , Kim Byeong Seop , Yan Yingjie , Chen Xiaojun , Wang Xuetong , Li Xin , Yang Xianxian , Wang Bingshun , Xu Haisong , Lin Li , Chai Gang 
Published: April 2023

Abstract
Background: Mandibular distraction osteogenesis (MDO) is a major part of the treatment for hemifacial microsomia patients. Due to the narrow surgical field of the intraoral approach, osteotomy accuracy is highly dependent on the surgeons’ experience. Electromagnetic (EM) tracking systems can achieve satisfying accuracy to provide helpful real-time surgical navigation. Our research team developed an EM navigation system based on artificial intelligence, which has been justified in improving the accuracy of osteotomy in the MDO in animal experiments. This study aims to clarify the effect of the EM navigation system in improving the MDO accuracy for hemifacial microsomia patients.
Methods:This study is designed as a single-centered and randomized controlled trial. Altogether 22 hemifacial microsomia patients are randomly assigned to the experiment group and control groups. All patients receive three-dimensional CT scans and preoperative surgical plans. The EM navigation system will be set up for those in the experiment group, and the control group will undergo traditional surgery. The primary outcome is the surgical precision by comparing the osteotomy position of pre- and postoperative CT scan images through the Geomagic Control software. The secondary outcomes include mandibular symmetry (occlusal plane deviation angle, mandibular ramus height and body length), pain scale and complications. Other indications, such as the adverse events of the system and the satisfactory score from patients and their families, will be recorded.
Discussion: This small sample randomized controlled trial intends to explore the application of an EM navigation system in MDO for patients, which has been adopted in other surgeries such as orthognathic procedures. Because of the delicate structures of children and the narrow surgical view, accurate osteotomy and protection of nearby tissue from injury are essential for successful treatment. The EM navigation system based on artificial intelligence adopted in this trial is hypothesized to provide precise real-time navigation for surgeons and optimally improve patients outcomes including function and aesthetic results. The results of this trial will extend the application of new navigation technology in pediatric plastic surgery.
Trial registration: Trial registration: Chinese Clinical Trial Registry: No. ChiCTR2200061565. Registered on June29, 2022.
Non-coding RNAs identification and regulatory networks in pathogen-host interaction in the microsporidia congenital infection
Authors: Shen Zigang , Yang Qiong , Luo Lie , Li Tangxin , Ke Zhuojun , Li Tian , Chen Jie , Meng Xianzhi , Xiang Heng , Li Chunfeng , Zhou Zeyang , Chen Ping , Pan Guoqing 
Published: April 2023

Abstract
Background：
The interaction networks between coding and non-coding RNAs (ncRNAs) including long non-coding RNA (lncRNA), covalently closed circular RNA (circRNA) and miRNA are significant to elucidate molecular processes of biological activities and interactions between host and pathogen. Congenital infection caused by vertical transmission of microsporidia N. bombyciscan result in severe economic losses in the silkworm-feeding industry. However, little is known that about ncRNAs that take place in the microsporidia congenital infection. Here we conducted whole-transcriptome RNA-Seq analyses to identify ncRNAs and regulatory networks for both N. bombycis and host including silkworm embryos and larvae during the microsporidia congenital infection.
Results:
A total of 4,171 mRNAs, 403 lncRNA, 62 circRNAs, and 284 miRNAs encoded by N. bombycis were identified, among which some differentially expressed genes formed cross-talk and are involved in N. bombycis proliferation and infection. For instance, a lncRNA/circRNA competing endogenous RNA (ceRNA) network including 18 lncRNAs, one circRNA, and 20 miRNAs was constructed to describe 14 key parasites genes regulation, such as polar tube protein 3 (PTP3), ricin-B-lectin, spore wall protein 4 (SWP4), and heat shock protein 90 (HSP90). Regarding silkworm host upon N. bombyciscongenital infection, a total of 14,889 mRNAs, 3,038 lncRNAs, 19,039 circRNAs, and 3,413 miRNAs were predicted based on silkworm genome with many differentially expressed coding and non-coding genes during distinct developmental stages. Different species of RNAs form interacting network to modulate silkworm biological processes, such as growth, metamorphosis and immune responses.Furthermore, a lncRNA/circRNA ceRNA network consisting of 140 lncRNAs, five circRNA, and seven miRNAs are constructed hypothetically to describe eight key host genes regulation, such as Toll-6, Serpin-6, inducible nitric oxide synthase (iNOS) and Caspase-8. Notably, cross-species analyses indicate that parasite and host miRNAs play a vital role in pathogen-host interaction in the microsporidia congenital infection.
Conclusion:
This is the first comprehensive pan-transcriptome study inclusive of both N. bombycis and its host silkworm with a specific focus on microsporidia congenital infection, and show that ncRNA-mediated regulation plays a vital role in the microsporidia congenital infection, which provides a new insight into understanding the basic biology of microsporidia and pathogen-host interaction.
Effects of C-S-Hs-PCE and Na2SO4 on hydration behavior of cement-lithium slag binder
Authors: He Yan , Chen Juan , Liu Shuhua , Jiang Mingjing , Hooton R.D. 
Published: April 2023

Abstract
Application of C-S-Hs-PCE and sodium sulfate into Portland cement containing 20 wt% lithium slag (LS) powder was investigated, in order to strengthen early mechanical properties. Synergistic effects of C-S-Hs-PCE and sodium sulfate on hydration properties and microstructure of cement-LS system were analyzed. Results showed that C-S-Hs-PCE was advantageous for modifying fluidity of fresh LS-cement binder, while increased dosage of sodium sulfate decreased dispersibility of fresh paste. C-S-Hs-PCE and sodium sulfate exhibited a synergistic effect on strength enhancement, hydration acceleration as well as setting behavior of LS-cement binder. Sodium sulfate increased alkalinity of interstitial solution and promoted dissolution of LS. Dissolved Al and Si from LS powder reacted with dissolved sulfate ions from sodium sulfate to produce extra hydrates, and C-S-Hs-PCE accelerated pozzolanic reaction as well as hydration reaction via nucleation effect collaborated with dispersing effect. C-S-Hs-PCE accelerated reaction process of sodium sulfate via nucleation effect, and activation effect of sodium sulfate provided more newly-formed hydrates to act as nucleation seeds or crystal skeleton for induce hydration of new phases. The accelerated hydration generated more AFt and C-S-H gel in the matrix. Newly formed hydrates promoted exceedingly the appearance of network, leading to a refinement of pore structure as well as enhancement in mechanical strength. Application of LS into cement as a greener binder could be obtained by synergistic adoption of C-S-Hs-PCE and sodium sulfate.
The Impact of a Love Language Game Intervention on Relationship Satisfaction Among Chinese Couples in China and Malaysia: Examining the Role of Individualism-Collectivism
Authors: Chen Guanzheng , Chen Yanying 
Published: April 2023

Abstract
Background:
Relationship satisfaction is crucial for the well-being and longevity of romantic relationships. Although love language interventions have demonstrated positive effects on relationship satisfaction, most research has focused on Western contexts. This study investigates the impact of a Love Language Game intervention on relationship satisfaction among Chinese couples in China and Malaysia and examines the role of individualism-collectivism in determining the intervention's effectiveness.
Methods:
Employing a mixed-methods approach and a randomized controlled trial design, this study incorporated both quantitative and qualitative components. The quantitative analysis utilized a two-way ANOVA to compare the Love Language Game intervention group with a control group participating in an alternative relationship enhancement workshop series. The qualitative component consisted of interviews exploring participants' experiences and perceptions of the interventions.
Results:
The findings revealed the effectiveness of the Love Language Game intervention in improving relationship satisfaction among Chinese couples and highlighted the influence of individualism-collectivism on the intervention's outcomes. Participants' experiences and perceptions emphasized the significance of engagement, communication, cultural sensitivity, and tailored strategies in relationship interventions.
Conclusions:
This study contributes to the literature on love language-based interventions and their application in culturally diverse settings, offering valuable insights for relationship counsellors, educators, and policymakers. The findings may guide the development and implementation of culturally sensitive interventions to enhance relationship satisfaction and overall well-being in diverse populations.
Applications of λ-dominance degree based rough sets in conflict problems
Authors: Rehman Noor , Ali Abbas , Liu Peide , Park Choonkil , Donganont Mana 
Published: April 2023

Abstract
Multi-attribute group decision making can be considered as the process of ranking alternatives or selecting an optimal alternative by many decision makers based on multiple criteria. By comparing any two alternatives pairwise, the preference relations provide efficient ways to represent the preference degrees of decision makers. The aim of this paper is to propose a general framework for the study of multi-attribute and multi decision information system by introducing the λ-dominance degree based rough set. Applying the notion of λ-dominance degree relation, we propose λ-dominance degree based rough set approach by substituting the dominance relation with λ-dominance degree relation. After employing λ-dominance degree based rough set approach to it for any times, we can only get six different sets at most. That is to say, every rough set in a universe can be approximated by only six sets, where the lower and upper approximations of each set in the six sets are still lying among these six sets. The relationships among these six sets are established. Another theme of the present study is to build two types of approximate precision, rough degree, approximate quality and approximate accuracy and their mutual relationship. Finally, two different algorithms for conflict situation are proposed and we compare the proposed models with other existing models.
A Case Report of Scrub Typhus in Third Trimester of Pregnancy Leading to Multi-organ Dysfunction – A Lesson Learned.
Authors: Singla Rimpi , Katoch Tanvi , Suri Vikas , Biswal Manisha 
Published: April 2023

Abstract

Introduction:
Scrub typhus in pregnancy may present with varying clinical spectrum ranging from mild febrile illness to multi-organ dysfunction. Severe form of untreated disease may result in maternal and/or fetal death. We present the case report of a primigravida in her third trimester of pregnancy with scrub typhus that faced “maternal near miss” and fetal demise.
Case:
A 25-year-old primigravidaat 36 weeks of gestation was referred to us with fever, headache, and malaise for 15 days and progressive dyspnea for two days. She was being treated with antipyretics and non-specific antibiotics before referral. She had tachycardia, hypotension, tachypnoea, orthopnea, oxygen saturation of 90% on room air, and generalized anasarca. Investigations revealed thrombocytopenia, deranged renal function tests, and increased procalcitonin. Azithromycin and ceftriaxone were started empirically. ELISA for Scrub typhus was positive. Subsequently, an eschar was found below left breast. Her condition improved with resuscitation, supportive therapy, and definitive treatment. Patient had spontaneous labor within 24 hours of admission resulting in vaginal delivery. She had intrapartum stillbirth.
After delivery, she again developed respiratory distress requiring intubation. The chest Xray showed a deteriorating picture with consolidation in right upper lung and bilateral reticulo-nodular opacities. Antibiotics were switched to meropenem and doxycycline 100mg twice a day. Fever subsided after day-4 postpartum and she was gradually weaned off from the ventilator.
Conclusions:
It is important to consider this differential early in the course of illness especially in tropical countries. Delay in diagnosis and hence, treatment can result in catastrophic outcome.
A molecular phylogeny of the subfamily Plusiinae (Lepidoptera: Noctuidae) in India inferred from mitochondrial and nuclear ribosomal DNA sequences
Authors: Sinha Twinkle , Shashank P. R. 
Published: April 2023

Abstract
Background The subfamily Plusiinae is an economically important moth pest group under species rich family Noctuidae (Lepidoptera). The evolutionary history of this important subfamily has not been resolved completely. Particularly in India, the genus is represented by a species complex, but the taxonomic delineation between them is still unclear.Methods and results In present study, a comprehensive phylogenetic relationship among Indian species of this subfamily has been inferred for the first time based on mitochondrial marker, Cytochrome Oxidase I (COI), and nuclear gene marker, Ribosomal Protein S5 (RPS5), emphasizing tribal level classification. Here, we analyzed 125 plusiinae taxa from eight biogeographical zones of India comprising 2 molecular markers: mitochondrial marker, Cytochrome Oxidase I (COI), and nuclear gene marker, Ribosomal Protein S5 (RPS5). The results revealed that Plusiinae tribes were monophyletic and considered sister groups that shared many derived characteristics. The ML/MP cladogram based on the barcoding gene successfully separates all the species, but not all tribes. While the nuclear gene marker, RPS5 separated all the species according to their tribes. The combined analysis of both genes showed tribe resolution into distinct clades.Conclusions This is the first comprehensive study on phylogenetic studies of 25 species of plusiinae from India which gives a clear information about species position and arrangement within taxa.