bluuebunny/crossref_metadata_2025_split huggingface dataset:
How metabolism and development are intertwined in space and time
Authors: Caldana Camila , Carrari Fernando , Fernie Alisdair R. , Sampathkumar Arun 
Published: July 2023

SUMMARYDevelopmental transitions, occurring throughout the life cycle of plants, require precise regulation of metabolic processes to generate the energy and resources necessary for the committed growth processes. In parallel, the establishment of new cells, tissues, and even organs, alongside their differentiation provoke profound changes in metabolism. It is increasingly being recognized that there is a certain degree of feedback regulation between the components and products of metabolic pathways and developmental regulators. The generation of large‐scale metabolomics datasets during developmental transitions, in combination with molecular genetic approaches has helped to further our knowledge on the functional importance of metabolic regulation of development. In this perspective article, we provide insights into studies that elucidate interactions between metabolism and development at the temporal and spatial scales. We additionally discuss how this influences cell growth‐related processes. We also highlight how metabolic intermediates function as signaling molecules to direct plant development in response to changing internal and external conditions.
The second‐generation curcumin analogue RL71 elicits G2/M cell cycle arrest and apoptosis in canine osteosarcoma cells
Authors: Kelly Barnaby , Thamm Douglas , Rosengren Rhonda J. 
Published: July 2023

AbstractCanine osteosarcoma is an aggressive cancer, comprising 85% of canine bone neoplasms. Current treatment practices of surgery and chemotherapy increase 1‐year survival by only 45%. The curcumin analogue RL71, has demonstrated potent in vitro and in vivo efficacy in several models of human breast cancer through increased apoptosis and cell cycle arrest. Thus, the present study aimed to investigate efficacy of curcumin analogues in two canine osteosarcoma cell lines. Osteosarcoma cell viability was assessed using the sulforhodamine B assay and mechanisms of action were determined by analysing the levels of cell cycle and apoptotic regulatory proteins via Western blotting. Further evidence was obtained using flow cytometry to detect cell cycle distribution and the number of apoptotic cells. RL71 was the most potent curcumin analogue with EC50 values of 0.64 ± 0.04 and 0.38 ± 0.009 μM (n = 3) in D‐17 (commercial) and Gracie canine osteosarcoma cells, respectively. RL71 significantly increased the ratio of cleaved‐caspase 3 to pro‐caspase 3 and the level of apoptotic cells at the 2× and 5× EC50 concentration (p < 0.001, n = 3). Furthermore, at the same concentration, RL71 significantly increased the number of cells in the G2/M phase. In conclusion, RL71 has potent cytotoxic activity in canine osteosarcoma cells triggering G2/M arrest and apoptosis at concentrations achievable in vivo. Future research should further investigate molecular mechanisms for these changes in other canine osteosarcoma cell lines prior to in vivo investigation.
Delay of definitive care in cats and dogs with gastrointestinal foreign body obstruction following antiemetic administration: 537 cases (2012–2020)
Authors: Puzio Claire E. , Rudloff Elke , Pigott Armi M. 
Published: July 2023

AbstractObjectiveTo determine whether administration of antiemetic medication to dogs and cats with gastrointestinal foreign body obstruction (GIFBO) delays time to definitive care (surgery or endoscopy) and increases the risk of complications.DesignRetrospective study (January 2012–July 2020).SettingPrivate referral center.AnimalsFive hundred and thirty‐seven (440 dogs and 97 cats).InterventionsNone.Measurements and Main ResultsMedical records of dogs and cats with GIFBO were reviewed for antiemetic administration at the onset of clinical signs, time from onset of clinical signs to first intervention and definitive care, GIFBO‐related complications, and length of hospitalization. Antiemetics were prescribed for 200 of 537 patients (158 dogs, 42 cats). Antiemetic administration was associated with an increased time between the onset of clinical signs and definitive care (3.2 days [95% confidence interval, CI, 2.8–3.5] vs. 1.6 days [95% CI, 1.4–2.0]; P < 0.001) but not with GIFBO‐associated complications (P = 0.45). Antiemetic administration was associated with an increased length of hospitalization (1.6 days [95% CI, 1.4–1.7] vs. 1.1 days [95% CI, 1.1–1.2]; P < 0.001). A longer duration of clinical signs prior to intervention was associated with GIFBO‐related complications (P < 0.001) regardless of antiemetic administration.ConclusionsAntiemetic administration in patients with GIFBO was associated with increased time to definitive care and length of hospitalization but not GIFBO‐associated complications. Antiemetics are not inherently contraindicated in patients for whom GIFBO is a differential, but clients should be counseled to monitor for progression of clinical signs and follow‐up accordingly.
Symplectic mapping class groups of blowups of tori
Authors: Smirnov Gleb 
Published: July 2023

AbstractLet be a Kähler form on the real 4‐torus . Suppose that satisfies an irrationality condition that can be achieved by an arbitrarily small perturbation of . This note shows that the smoothly trivial symplectic mapping class group of the one‐point symplectic blowup of is infinitely generated.
Retrospective cohort study to examine the association between serum amylase and the incidence of type 2 diabetes mellitus, Toranomon Hospital Health Management Center Study 23 (TOPICS 23)
Authors: Ikeda Izumi , Fujihara Kazuya , Osawa Taeko , Takeda Yasunaga , Hatta Mariko , Matsubayashi Yasuhiro , Kodama Satoru , Mori Yasumichi , Kadowaki Takashi , Honda Ritsuko , Arase Yasuji , Sone Hirohito 
Published: July 2023

IntroductionLow serum amylase values are cross-sectionally associated with the prevalence of type 2 diabetes mellitus (T2DM) but have not been shown to be longitudinally associated with its incidence. This retrospective cohort (ie, historical cohort) study aimed to examine the association of previously lowered levels of serum amylase with incident T2DM.Research design and methodsExamined were 8316 individuals who had annual health examinations for 6 years (ie, 7 times) at the Toranomon Hospital Health Management Center. The trajectory of serum amylase as the study exposure was classified into two elements: (1) serum amylase level at entry and (2) change in serum amylase, which was expressed as the annual change rate. The annual change rate was calculated by dividing the change in the amylase values according to follow-up periods. Regression analyses were performed to examine the association between low and decreased levels of serum amylase and the incidence of T2DM.ResultsAnalyzed were 6917 individuals who had not developed T2DM within 1 year after cohort entry. T2DM thereafter occurred in 1021 patients. Cox regression indicated that the adjusted HR (95% CI) for incident T2DM for amylase ≤57 IU/L (quintile (Q) 1) was 0.97 (0.84 to 1.13) compared with amylase ≥58 IU/L (Q2–Q5). Logistic regression indicated that the adjusted OR (95% CI) for an annual change rate of amylase ≤−2.0% (Q1) vs ≥−1.9% (Q2–Q5) was 3.53 (3.00 to 4.16). The adjusted ORs were consistently significant throughout sensitivity analyses according to baseline amylase and the combination of age, body mass index, and hemoglobin A1c.ConclusionsResults showed that not low but previously decreased serum amylase was a risk factor for T2DM, suggesting the significance of periodic examinations of serum amylase values to detect individuals at high risk of T2DM.
Pregnancy, fetal, and neonatal outcomes after a first booster dose of covid-19 vaccine during pregnancy in Ontario, Canada: population based, retrospective cohort study
Authors: Fell Deshayne B , Dimanlig-Cruz Sheryll , Török Eszter , Håberg Siri E , Regan Annette K , Kaufman Jay S , Platt Robert W , Gravel Christopher A , Bruce Liam , Shah Prakesh S , Wilson Kumanan , Sprague Ann E , Alton Gillian D , Dhinsa Tavleen , El-Chaâr Darine , Buchan Sarah A , Kwong Jeffrey C , Wilson Sarah E , Dunn Sandra I , MacDonald Shannon E , Barrett Jon , Okun Nannette , Walker Mark C 
Published: July 2023

ObjectiveTo assess risk of adverse pregnancy, fetal, and neonatal outcomes after a third dose (first booster dose) of covid-19 vaccine during pregnancy among individuals who had completed both doses of primary covid-19 vaccine series before pregnancy.DesignPopulation based, retrospective cohort study.SettingOntario, Canada, from 20 December 2021 to 31 August 2022.ParticipantsIndividuals were included if they were pregnant with an expected date of delivery from 20 December 2021 (start date of third dose eligibility for everyone ≥18 years) to 31 August 2022, who had completed the two doses of primary covid-19 messenger RNA vaccine series before pregnancy, and became eligible for a third dose (≥six months since dose two) before the end of pregnancy.Main outcome measuresPregnancy outcomes included hypertensive disorders of pregnancy, placental abruption, caesarean delivery, chorioamnionitis, and postpartum hemorrhage. Fetal and neonatal outcomes included stillbirth, preterm birth, admission to neonatal intensive care unit for >24 h, newborn 5 min Apgar score <7, and small-for-gestational age infant (<10th percentile). We estimated hazard ratios and 95% confidence intervals for study outcomes, treating dose three as a time varying exposure and adjusting for confounding using inverse probability weighting.ResultsAmong 32 689 births, 18 491 (56.6%) were born to individuals who received a third covid-19 dose during pregnancy. Compared with eligible individuals who did not receive a third dose during pregnancy, no increased risks were associated with receiving a third covid-19 vaccine dose during pregnancy for placental abruption (adjusted hazard ratio 0.84 (95% confidence interval 0.70 to 1.02)), chorioamnionitis (0.67 (0.49 to 0.90)), postpartum haemorrhage (1.01 (0.89 to 1.16)), caesarean delivery (0.90 (0.87 to 0.94)), stillbirth (0.56 (0.39 to 0.81)), preterm birth (0.91 (0.84 to 0.99)), neonatal intensive care unit admission (0.96 (0.90 to 1.03)), 5 min Apgar score<7 (0.96 (0.82 to 1.14)), or small-for-gestational age infant (0.86 (0.79 to 0.93)).ConclusionReceipt of a third covid-19 vaccine dose during pregnancy was not associated with an increased risk of adverse pregnancy, fetal, or neonatal outcomes. These findings can help to inform evidence based decision making about the risks and benefits of covid-19 booster doses during pregnancy.
Burden of COPD in China and the global from 1990 to 2019: a systematic analysis for the Global Burden of Disease Study 2019
Authors: Li Min , Chen Hanxiang , Zhou Na , Zhang Ping , Duan Shengnan , Wu Taihua , Yi Yuanyuan , Yuan Ni 
Published: July 2023

ObjectiveTo investigate the current disease burden of chronic obstructive pulmonary disease (COPD) in China and globally using the Global Burden of Disease (GBD) data in 2019, as well as to analyse the changes in its risk factors, providing a scientific basis for the formulation of a comprehensive prevention and control strategy for COPD in China.Study designAn observational study based on the GBDs.MethodsBased on the GBD 2019 database, we obtained data on incidence, prevalence, mortality, disability-adjusted life years (DALYs) and corresponding age-standardised rates of COPD in China and the global, and analysed and described the changing trends of COPD burden in China and the global from 1990 to 2019.ResultsIn 2019, the total number of COPD deaths in China was 1.04 (95% uncertainty intervals (95% UI): 0.89–1.27) million cases, the number of patients with COPD was 45.16 (95% UI: 41.13–49.62) million cases, and the number of new cases was 4.0 (95% UI: 3.6–4.4) million cases. DALYs were 74.4 (95% UI: 68.2–80.2) million years. Compared with 1990, the number of new incident cases and the overall prevalence of COPD in China in 2019 increased by 66.20% and 66.76%, respectively, which is lower than the overall global level.ConclusionFrom 1990 to 2019, the age-standardized prevalence rate (ASPR), the age-standardized incidence rate (ASIR) and the age-standardized death rate (ASDR) in China and the global all showed a downward trend, and the rate of decline in China was much higher than the overall level of the world, indicating that China has made specific achievements in the prevention and treatment of COPD, but overall the disease burden of COPD is still hefty, and the number of affected individuals is still increasing.
The perils and promises of generative artificial intelligence in neurointerventional surgery
Authors: Ray Tyler R , Kellogg Ryan T , Fargen Kyle M , Hui Ferdinand , Vargas Jan 
Published: July 2023

Generative artificial intelligence (AI) holds great promise in neurointerventional surgery by providing clinicians with powerful tools for improving surgical precision, accuracy of diagnoses, and treatment planning. However, potential perils include biases or inaccuracies in the data used to train the algorithms, over-reliance on generative AI without human oversight, patient privacy concerns, and ethical implications of using AI in medical decision-making. Careful regulation and oversight are needed to ensure that the promises of generative AI in neurointerventional surgery are realized while minimizing its potential perils.[ChatGPT authored summary using the prompt “In one paragraph summarize the promises and perils of generative AI in neurointerventional surgery”.]
New insights into endophytic fungi diversity and their potential correlation with polyphyllin levels of Paris polyphylla var. yunnanensis
Authors: Lan Juan , Liu Ting , Miao Liyun , Pei Ting , Gan Zhe , Lin Aihua , Geng Hong , Zhang Peng 
Published: July 2023

Endophytes confer fitness advantages to host plants. However, the ecological communities of endophytic fungi in the different tissues (rhizomes, stems, and leaves) of Paris polyphylla and the relationship of their endophytic fungi with polyphyllin levels remain unclear. In this study, the community diversity and differences of endophytic fungi in the rhizomes, stems, and leaves of P. polyphylla var. yunnanensis were investigated, and a comprehensively diverse community of endophytic fungi was represented, including 50 genera, 44 families, 30 orders, 12 classes, and 5 phyla. Distributions of endophytic fungi differed greatly across the three tissues, with six genera common to all tissues, and 11, 5, and 4 genera specific to the rhizomes, stems, and leaves, respectively. Seven genera showed a significantly positive correlation to polyphyllin contents, indicating their potential roles in polyphyllin accumulation. This study provides valuable information for further research of the ecological and biological functions of endophytic fungi of P. polyphylla.
Extended quasiparticle Padé approximation for non-Fermi liquids
Authors: Morawetz Klaus 
Published: July 2023

Abstract
The extended quasiparticle picture is adapted to non-Fermi systems by suggesting a Padé approximation which interpolates between the known small scattering-rate expansion and the deviation from the Fermi energy. The first two energy-weighted sum rules are shown to be fulfilled independent of the interpolating function for any selfenergy. For various models of one-dimensional Fermions scattering with impurities the quality of the Padé approximation for the spectral function is demonstrated and the reduced density matrix or momentum distribution is reproduced not possessing a jump at the Fermi energy. Though the two-fold expansion is necessary to realize the spectral function and reduced density, the extended quasiparticle approximation itself is sufficient for the description of transport properties due to cancellation of divergent terms under integration. The T-matrix approximation leads to the delay time as the time two particles spend in a correlated state. This contributes to the reduced density matrix and to an additional part in the conductivity which is presented at zero and finite temperatures. Besides a localization at certain impurity concentrations, the conductivity shows a maximum at small temperatures interpreted as onset of superconducting behaviour triggered by impurities. The Tan contact reveals the same universal behaviour as known from electron–electron scattering.

Graphic abstract
ОЦІНЮВАННЯ ПОВНОТИ РЕЄСТРАЦІЇ КЛІЩОВИХ ІНФЕКЦІЙ НА ТЕРНОПІЛЛІ
Authors: Паничев В. О. , Андрейчин М. А. , Сверстюк А. С. 
Published: July 2023

Мета дослідження – оцінити повноту статистичного обліку окремих кліщових інфекцій.
Матеріали і методи. Використано результати багаторічного епідеміологічного нагляду за кліщовими інфекційними хворобами в Тернопільській області, що включає дані офіційної статистики, епідеміологічних розслідувань, польових ентомологічних, лабораторних досліджень. Застосовано статистичні методи оцінки та прогнозування.
Результати. Оцінено прояви епідемічного процесу, клінічного перебігу, виявлення Лайм-бореліозу (ЛБ) у Тернопільській області. Проаналізовано показники захворюваності в окремих районах та їх динаміку протягом 2000-2021 рр. Порівняно кількість зареєст­рованих випадків з прогнозованими статистичними даними. Проаналізовано співвідношення еритемних (ЕФ) і безеритемних форм (БЕФ) ЛБ, виявлено територіальні відмінності в діагностуванні. Визначена осередковість. Оцінено повноту обліку подій ризику зараження бореліями.
Рівні зареєстрованої захворюваності на ЛБ виявилися нижчими від прогнозованих. Водночас прогнозована кількість недовиявлених хворих на БЕФ ЛБ була вища за число недовиявлених випадків ЕФ.
Висновок. Здійснене оцінювання дає підстави вважати офіційні статистичні показники захворюваності на ЛБ нижчими за реальні. Досі офіційно не реєструються інші та поєднані кліщові інфекції.
SYNERGISTIC ANTIMICROBIAL ACTIVITY BY COMBINING TERBINAFINE WITH BENZOYL PEROXIDE AGAINST САNDIDA ALBICANS AND STAPHYLOCOCCUS AUREUS
Authors: Kochnieva O. V. , Kotsar O. V. , Kalashnyk-Vakulenko Y. M. 
Published: July 2023

The aim. To investigate the ability of microorganisms’ C. albicans and S. aureus to form biofilms. To evaluate the sensitivity of biofilms to combination of terbinafine and benzoyl peroxide on this association.
Material and methods. The ability to form biofilms of microorganisms and the antimicrobial effect of the studied drugs was carried out on polystyrene plates for enzyme-linked immunosorbent assay. The optical density (OD) of biofilms was measured at a wavelength of 545 nm on a biochemical analyzer. The viability of microorganisms was determined by counting the number of colony-forming units (CFU) in 1 ml of the culture medium with experimental strains.
Results. The average optical density of biofilms was (1.0892±0.006) units. The OD for clinical isolates was (0.0776±0.004) units. It has been proven that combination of the antimycotic substance − terbinafine and the antiseptic − benzoyl peroxide had a high activity in relation to the association of C. albicans and S. aureus with concentration of 1.3 μg/ml.
Conclusion. The study showed the ability to form biofilms in clinical strains of microorganisms is more pronounced than the reference strains. The highest rate of biofilm formation was found in the association of microorganisms’ C. albicans and S. aureus. The combination of medicines also effectively operated with planktonic forms of bacteria, but also on microorganisms mobilized in biofilms. A decrease of optical density and 2-fold decrease of CFU demonstrated this.
ВПЛИВ ЕЛЕКТРОМАГНІТНОГО ВИПРОМІНЮВАННЯ МОБІЛЬНОГО ТЕЛЕФОНУ НА ОРГАНІЗМ ЛЮДИНИ
Authors: Стравський Я. С. , Федонюк Л. Я. , Подобівський С. С. , Ярема О. М. , Стравський Т. Я. 
Published: July 2023

Мета: проаналізувати дані літератури та визначити вплив електромагнітних випромінювань на організм людини.
Матеріали і методи. За допомогою пошукової бази даних мережі «Інтернет» (зокрема, Google академія) та наукових статей було проаналізовано особливості впливу електромагнітного випромінювання на організм людини.
Результати. Описано природні джерела електромагнітного випромінювання. Вказано, що процес взаємодії ЕМВ із живими організмами може бути подано у вигляді фаз. 1-шa фаза – поглинання енергії ЕМВ клітинами живого організму у вигляді розриву міжмолекулярних з’єднань і руйнування ДНК. 2-га фаза – перетворення енергії ЕМВ у хімічну енергію з утворенням іонів і вільних активних радикалів. 3-тя фаза – розвиток радіохімічних реакцій у живому організмі, що опромінюється. Зазначено, що акустичні (звукові) сигнали викликають легкі форми пухлин слухового нерва. Пухлини виникають в 100 тис. людей у рік і можуть призвести до глухоти, а у Великобританії приблизно в 4700 осіб виявляються пухлини мозку. Число людей – користувачів мобільного зв’язку, у яких з’явилася пухлина мозку, за останні 30 років збільшилося на 45 %, а якщо мобільний телефон постійно прикладається до однієї й тієї ж частини голови, ризик зростає в 4 рази. За твердженням американських вчених, тривале регулярне носіння «мобільника» в кишенях здатне на 33 % знизити репродуктивні здібності чоловіка. Наведено дані про те, що низькоінтенсивне радіочастотне випромінювання діє як стресовий/оксидативний чинник для клітини, призводить до розвитку лейкемії, а користування мобільним телефоном понад 1 годину на день погіршує зір і слух.
Особливу увагу відведено вибору мобільного телефону та правилам користування ним. Вказано, що Міністерство освіти і науки України та національний оператор мобільного зв’язку «Київстар» підписали Меморандум про співробітництво в рамках соціальної програми «Київстар»-«Мобільна культура».
Висновок. Мобільний телефон є необхідним атрибутом життя людини за умови дотримання всіх правил його використання.
CARDIOVASCULAR DISEASES IN UKRAINE UNDER CONDITIONS OF GLOBAL WARMING
Authors: Vadzyuk S. N. , Huk V. O. 
Published: July 2023

Рurpose: to establish the relationship between cardiovascular diseases in Ukraine and an increase in the average annual temperature.
Materials and Мethods. The data of the State Statistics Service of Ukraine on the number of cases of cardiovascular diseases from 1990 to 2013, as well as on the share of the urban and rural population in various regions for 1990, 2000 and 2013 were analyzed. Statistical data starting from 2014 were not used due to their absence from the temporarily occupied territories of Ukraine. Average annual temperatures were obtained from the global database – The Global Historical Climatology Network. With the help of the National reports on the state of the natural environment in Ukraine, atmospheric air pollution in the regions of Ukraine was assessed. Statistical processing of the obtained results was carried out using correlation and regression analysis with determination of the reliability of the approximation.
Results. A direct average correlation between average annual temperatures and the number of newly registered cases of cardiovascular diseases in Ukraine between 1990 and 2013 was established (correlation coefficient +0.5). After carrying out an analysis between the regions of Ukraine, it was established that in the regions with direct strong and medium correlations between the number of newly detected diseases of the circulatory system and average annual air temperatures (Dnipropetrovsk, Zaporizhzhya, Luhansk, Kharkiv, Donetsk, Autonomous Republic of Crimea, Kyiv, Sumy, Kherson, Odesa, Mykolaiv, Kirovohrad, Lviv, Zhytomyr regions) the urban population prevails, as well as a higher air pollution index. In other regions of Ukraine, where the rural population predominates, a weak straight line is established, as well as inverse correlation dependences and a lower level of atmospheric air pollution.
Conclusions. In recent decades, the average annual temperature of the environment has been increasing, which has a negative effect on human health, in particular on the cardiovascular system, causing its damage. Therefore, it is important to start acting now to avoid the negative consequences of global warming.