bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contrasting diversity patterns between microeukaryotic and prokaryotic communities in the cold-seep sediments
Authors: Xu Zhimeng , Chen Jiawei , Liang Wenzhao , Chen Zhao Liang , Wu Wenxue , Xia Xiaomin , Chen Bingzhang , He Ding , Liu Hongbin 
Published: January 2025

Abstract
Cold seeps are hotspots of biodiversity. However, the quantification of the microbial diversity, particularly that of microeukaryotes, remains scarce and little is known about the active groups. Here we investigated the diversity and activity of prokaryotes and microeukaryotes in the Haima cold seep sediments in northern South China Sea using both DNA (whole community) and RNA (active community) signatures. We found that, in general, prokaryotes had lower diversity in the seep sediment than in non-seep regions while microeukaryotes showed the opposite pattern. This could be explained by the dominance of homogeneous selection in the prokaryotic community while microeukaryotic communities were less affected by environmental selection, harboring high richness of abundant groups in the seep regions. Compositional difference between DNA and RNA communities was much larger in microeukaryotes than prokaryotes, which could be reflected by the large number of inactive microeukaryotic taxa. Compared to whole community, the seep-active groups, e.g., Breviatea, Labyrinthulomycetes and Apicomplexa in microeukaryotes, were more sensitive to and directly influenced by environmental factors, suggesting their pivotal roles in ecosystem biodiversity and functions. This study provides insight into the distinct diversity patterns and regulating mechanisms between prokaryotic and microeukaryotic communities in cold-seep sediments, deepening our understanding of microbial ecology in deep-sea extreme habitats.
Medium and Managerial Expertise: Orthogonal Drawing and Advertising in T.M. Cleland’s The New Cadillac (1928)
Authors: Golec Michael J 
Published: January 2025

Abstract
In the first few decades of the twentieth century, mechanical drawing systems were utilized for advertising and marketing campaigns. This article examines an unremarked on episode of this early history: T.M. Cleland’s illustrations for The Book of Locomobile (1917) and The New Cadillac (1928), a booklet that appeared as an advertising supplement in Scribner’s and the Saturday Evening Post. The examples of advertising design addressed in this article are notable for the designer’s intentional integration of technical knowledge and visual practices of his audience, which was mostly made up of engineers and a growing managerial class. Designing with his audience’s technical and visual acuities as his medium, Cleland’s advertising illustrations took a mechanical ideal and transcribed it into a mercantile ideal that brought a technically informed audience into close harmony with a commercially created order. Importantly, this article contributes to the literature on the history of advertising design by introducing the concept of a beholder’s “skillful interpretation” to investigate an order of technically inflected perception within the context of advertising’s commercialization of attention.
Spatial effect detection regression for large-scale spatio-temporal covariates
Authors: Zhang Chenlin , Zhou Ling , Guo Bin , Lin Huazhen 
Published: January 2025

Abstract
We develop a Spatial Effect Detection Regression (SEDR) model to capture the nonlinear and irregular effects of high-dimensional spatio-temporal predictors on a scalar outcome. Specifically, we assume that both the component and the coefficient functions in the SEDR are unknown smooth functions of location and time. This allows us to leverage spatially and temporally correlated information, transforming the curse of dimensionality into a blessing, as confirmed by our theoretical and numerical results. Moreover, we introduce a set of 0–1 regression coefficients to automatically identify the boundaries of the spatial effect, implemented via a novel penalty. A simple iterative algorithm, with explicit forms at each update step, is developed, and we demonstrate that it converges from the initial values given in the paper. Furthermore, we establish the convergence rate and selection consistency of the proposed estimator under various scenarios involving dimensionality and the effect space. Through simulation studies, we thoroughly evaluate the superior performance of our method in terms of bias and empirical efficiency. Finally, we apply the method to analyse and forecast data from environmental monitoring and Alzheimer’s Disease Neuroimaging Initiative study, revealing interesting findings and achieving smaller out-of-sample prediction errors compared to existing methods.
Synthesizing Surveys with Multiple Units of Observation: An Application to the Longitudinal Aging Study in India
Authors: Snoke Joshua , Meijer Erik , Phillips Drystan , Wilkens Jenny , Lee Jinkook 
Published: January 2025

Abstract
We present methodology for creating synthetic data and an application to create a publicly releasable synthetic version of the Longitudinal Aging Study in India (LASI). The LASI, a health and retirement survey, is used for research and educational purposes, but it can only be shared under restricted access due to privacy considerations. We present novel methods to synthesize the survey, maintaining three nested levels of observation—individuals, couples, and households—with both continuous and categorical variables and survey weights. We show that the synthetic data maintains the distributional patterns of the confidential data and largely mitigates identification and attribute disclosure risk. We also present a novel method for controlling the risk and utility tradeoff for the synthetic data that take into account the survey sampling rates. Specifically, we down-weight records that have a high likelihood of being uniquely identifiable in the population due to unique demographic information and oversampling. We show this approach reduces both identification and attribute risk for records while preserving better utility over another common approach of coarsening records. Our methods and evaluations provide a foundation for creating a synthetic version of surveys with multiple units of observation, such as the LASI.
Role of dehydrated human amnion/chorion membrane in enhancing functional outcomes after robot-assisted radical prostatectomy: a systematic review and meta-analysis
Authors: Yang Yun-Jung , Kim Taehyen , Yang Eun-Jung , Choi Se Young 
Published: January 2025

Abstract

Background
Dehydrated human amnion/chorion membrane (dHACM) has shown potential in enhancing neurovascular recovery and functional outcomes in robot-assisted radical prostatectomy (RARP).

Aim
To evaluate the effects of dHACM on continence recovery, sexual function, and oncological outcomes in patients undergoing RARP.

Methods
A systematic review and meta-analysis were conducted following PRISMA guidelines, analyzing data from PubMed, Cochrane, and EMBASE. Six retrospective studies comparing RARP with and without dHACM were included. Odds ratios (OR) and standardized mean differences (SMD) were calculated using a random-effects model.

Outcomes
The primary outcomes were continence and potency recovery, and secondary outcomes included biochemical recurrence (BCR).

Results
The meta-analysis included 4072 patients (1699 experimental and 2373 control). dHACM significantly improved early continence recovery (SMD 1.78, 95% CI 1.26–2.34) at &lt;3, 3, and 6 months postoperatively (OR 1.95, 95% CI 1.13–3.36; OR 2.17, 95% CI 1.52–3.09; and OR 1.70, 95% CI 1.10–2.63, respectively). Time to potency recovery was shorter (SMD –0.55, 95% CI –0.67 to –0.43), with significant improvements at &lt;3, 3, 6, and 9 months (OR 1.67, 95% CI 1.25–2.23; OR 1.27, 95% CI 1.06–1.53; OR 1.41, 95% CI 1.15–1.72; and OR 1.51, 95% CI 1.16–1.97, respectively). There were no significant differences in BCR (OR 0.85, 95% CI 0.54–1.35).

Clinical Implications
dHACM offers potential as an adjunct to enhance functional recovery following RARP without compromising oncologic safety, but further high-quality studies are needed.

Strengths & Limitations
Strengths include a comprehensive analysis of early functional outcomes and low heterogeneity in early potency and continence data. Limitations include reliance on retrospective studies and lack of randomized controlled trials.

Conclusion
dHACM may accelerate continence and sexual function recovery in early period after RARP while maintaining oncological outcomes, but further randomized studies are necessary to confirm these findings.
A cross-sectional study characterizing the prevalence of utility service outages across demographic characteristics and health correlates in New York City
Authors: Do Vivian , Yuan Ariel , Lane Kathryn , Mantey Lauren Smalls , Siegel Eva , Olson Carolyn , Daouda Misbath , Casey Joan A. , Hernández Diana 
Published: January 2025

Background:
Utility services for electricity, gas, heat, and hot water are necessities for everyday activities (e.g., lighting, cooking, and thermal safety). Utility outages can threaten health; however, information is limited on the prevalence of electricity, gas, heat, and hot water outages in representative studies. We characterized infrastructure-related electricity, gas, heat, and hot water outages in New York City (NYC) and within subgroups.

Methods:
Using a representative 2022 survey of NYC adults (18+), we assessed the prevalence for 6+ hour utility outages and compared across building, demographic, and health subgroups. Building characteristics included age, number of floors, rental type, and owner/rental status. Demographics included household poverty, neighborhood poverty, and race/ethnicity. For health, we focused on cognitive impairment, electricity-dependent medical equipment use, and mental health conditions.

Results:
Outages impacted 20% of NYC residents. Heat outages were nearly 3× and 2× more common in mid-rise and high-rise buildings respectively, vs. low-rise buildings. Similarly, hot water outages were 5× and over 6× more prevalent in mid-rise and high-rise residences. Renters faced 2× more heat and hot water outages compared with owners. Compared with low-poverty households, high-poverty households faced 2× more hot water outages. Residents with mental health conditions experienced more electricity (11% vs. 5%), heat (15% vs. 7%), and hot water (16% vs. 8%) outages compared with those without.

Conclusions:
NYC utility outage prevalence varied by type with heat and hot water being most common. Disparities across building, sociodemographic, and health characteristics were also larger and more frequent for heat and hot water outages.
Dexamethasone Intravitreal Injection in Diabetic Patients Undergoing Cataract Surgery: an updated literature review
Authors: Meduri Alessandro , De Luca Laura , Oliverio Giovanni William , Mancini Maura , Minutoli Letteria , Silvagno Francesca , Bergandi Loredana , Aragona Pasquale 
Published: January 2025

Purpose:
This literature review synthetizes current evidence on the timing and efficacy of dexamethasone intravitreal injections for diabetic macular edema (DME) in patients undergoing cataract surgery, particularly phacoemulsification, to determine the optimal timing for improved outcome.

Methods:
A systematic review of the literature was conducted across key databases to identify peer-reviewed studies, clinical trials, and meta-analyses addressing dexamethasone injections administered pre-, intra-, and post-operatively for DME in the context of cataract surgery. Studies were selected based on relevance to timing, visual outcomes, and inflammation control, with a focus on comparative efficacy.

Results:
The findings suggest that timely dexamethasone injections can substantially reduce inflammation and enhance visual recovery for patients with DME undergoing cataract surgery. Studies indicate that preoperative injections may effectively dampen the inflammatory response triggered by surgical trauma, potentially preserving retinal integrity, while intraoperative and postoperative administrations contribute to sustained anti-inflammatory effects during the recovery phase. Comparative studies also highlight dexamethasone’s advantages over other anti-inflammatory treatments, such as NSAIDs, particularly in preventing cystoid macular edema. Notably, there was considerable variation in dosage and timing across studies, underscoring the need for standardized treatment protocols.

Conclusions:
Dexamethasone intravitreal injections offer a valuable intervention for managing DME in diabetic patients undergoing cataract surgery, with optimal timing playing a crucial role in maximizing therapeutic benefits. Preoperative injections appear to be particularly beneficial in reducing the risk of postoperative inflammatory complications. Further research should focus on developing comprehensive guidelines for timing and dosage to standardize treatment and improve patient outcomes in this high-risk population.
Performance of baseline quartile-stratified minimal clinically important difference estimates was superior to individual minimal clinically important difference estimates when compared with a gold standard comparator of important change
Authors: Riddle Daniel L. , Dumenci Levent 
Published: January 2025

Abstract
A variety of minimal clinically important difference (MCID) estimates are available to distinguish subgroups with differing outcomes. When a true gold standard is absent, latent class growth curve analysis (LCGC) has been proposed as a suitable alternative for important change. Our purpose was to evaluate the performance of individual and baseline quartile-stratified MCIDs. The current study included data from 346 persons with baseline and 12-month postoperative outcome data from KASTPain, a no-effect randomized clinical trial conducted on persons with knee arthroplasty and pain catastrophizing. Subgroup trajectories from LCGC were used as a gold standard comparator. Minimal clinically important difference–specific trajectories of recovery were calculated for the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) Pain, Disability and EuroQol-5 Dimension Visual Analogue Scale of self-reported health. The latent Kappa (K
l
) chance-corrected agreement between MCIDs and LCGCs were estimated to indicate which MCID method was best at detecting important change. For all 3 outcomes, the average latent class probabilities ranged from 0.90 to 0.99, justifying the use of LCGCs as a gold standard. The K
l
for LCGC and individual MCIDs ranged from 0.21 (95% CI = 0.13, 0.28) to 0.52 (95% CI = 0.41, 0.66). Baseline quartile-stratified K
l
for WOMAC Pain and Disability were 0.85 (95% CI = 0.78, 0.92) and 0.74 (95% CI = 0.68, 0.83), respectively. Classification errors in individual MCID estimates most likely result from ceiling effects. Minimal clinically important differences calculated for each baseline quartile are superior to individually calculated MCIDs and should be used when latent class methods are not available. Use of individual MCIDs likely contribute substantial error and are discouraged for clinical application.
Molecular and cellular targets of GABAergic anesthetics in the mesopontine tegmentum that enable pain-free surgery
Authors: Baron Mark , Vaso Kristina , Ibraheem Angham , Minert Anne , Devor Marshall 
Published: January 2025

Abstract
The mesopontine tegmental anesthesia area (MPTA) is a focal brainstem locus which, when exposed to GABAergic agents, induces brain-state transitioning from wakefulness to unconsciousness. Correspondingly, MPTA lesions render animals relatively insensitive to GABAergic anesthetics delivered systemically. Using chemogenetics, we recently identified a neuronal subpopulation within the MPTA whose excitation induces this same pro-anesthetic effect. However, very few of these “effector-neurons” express synaptic γ
2-containing GABAA receptor isoforms and none express extrasynaptic δ-subunit containing receptors, suggesting that they are not the direct cellular target of GABAergic agents. Here we used pharmacological tools in rats to define the molecular target(s) of GABAergics in the MPTA. GABA microinjected into the MPTA at nanomolar concentrations, selective for GABAA
δ-Rs, proved to be pro-anesthetic as was blocking GABA reuptake. Likewise, low-concentration gaboxadol/THIP, also selective for GABAA
δ-Rs, was effective, whereas benzodiazepines and zolpidem, which selectively target GABAA
γ
2-Rs, were not. The GABAergic anesthetics pentobarbital and propofol proved pro-anesthetic when applied to the MPTA at the low concentrations present in the brain after systemic dosing. Glycinergic agonists which are inhibitory, but infective on GABAA
δ-Rs, and other non-GABAergic agonists tested, were at most only marginally effective. We conclude that GABAA
δ-Rs are the primary molecular target of GABAergic anesthetics in the MPTA. Immunolabeling revealed that this GABAA-R isoform is expressed exclusively by a distinct subpopulation of MPTA “δ-cells” that reside in close apposition to effector neurons. This suggests that during wakefulness, δ-cells serve as inhibitory interneurons which, when silenced by GABAergic agents, disinhibit (excite) the effector-neurons, triggering transition to unconsciousness.
Updating an HIV Knowledge Questionnaire With Pre-exposure Prophylaxis Through Expert Opinions in the United States Using Delphi Methodology: A Qualitative Study
Authors: Min Se Hee , Kempf Mirjam-Colette , Johnson Amy Kristen , Wingood Gina , Klitzman Robert , Hughes Tonda L. , McDonald Victoria , Lee Corcoran Jessica , Dionne Jodie A. , Liu Jianfang , Schnall Rebecca 
Published: January 2025

Abstract
Our study was designed to update the HIV Knowledge Questionnaire by incorporating pre-exposure prophylaxis (PrEP) knowledge questions, as previous HIV knowledge tools lack this focus. Four rounds of Delphi surveys were conducted with 47 expert participants, each with extensive HIV-related expertise (mean experience: 18.94 years). Expert participants rated item relevance on a scale of 0–3 and provided feedback. An item was removed if over 70% scored it two or less. The process continued until consensus was reached, defined as 80% of items scoring above two by more than 70% of expert participants, with no new suggestions. The final measure included 31 items covering general HIV knowledge, transmission modes, treatment, risk, and PrEP definitions, types, and dosages. Future research is essential to validate this tool for research and clinical settings, ensuring it accurately measures patients' diverse knowledge levels.
Can Multiple Sclerosis-Related Symptoms Determine Sleep Disorders?
Authors: Koçak Ayyüce Tuba , Uçar Ayşe , Arslan Selda , Uca Ali Ulvi 
Published: January 2025

ABSTRACT

BACKGROUND: Sleep disorders are common and various in persons with multiple sclerosis (PwMS). However, their assessment and management remain in the background compared with motor symptoms. This study was conducted to better understand sleep disorders in PwMS and to analyze sleep problems and their predictive symptoms. METHODS: This cross-sectional design study was conducted with 136 PwMS. Data were collected using a participant identification form, Pittsburgh Sleep Quality Index, Insomnia Severity Index, Epworth Sleepiness Scale, and Stop-Bang Test. In data analysis, 1-way analysis of variance, t test and Pearson correlation, and multiple regression analysis were used. RESULTS: The study found that 51.5% of the participants have poor sleep quality, 36.8% have insomnia, 24.3% have excessive daytime sleepiness, and 19.9% were at risk of obstructive sleep apnea. Symptoms of forgetfulness (β = 0.199), depression (β = 0.223), pain (β = 0.278), and increased urinary frequency at night (β = 0.236) explain approximately 40% of the change in sleep quality score; difficulty swallowing (β = 0.218), depression (β = 0.253), and pain (β = 0.313) symptoms explain 33% of the change in insomnia score; fatigue (β = 0.292) and anxiety (β = 0.409) symptoms explain 32% of the change in sleepiness score. CONCLUSIONS: PwMS start to experience sleep disorders in the early stages of the disease, some characteristics affect sleep disorders, and the symptoms they experience explain sleep disorders at significant rates. Sleep disorders should be evaluated by nurses and interdisciplinary healthcare teams from the early stages of multiple sclerosis with different aspects and to include predictive symptoms in the interventions to be implemented to reduce sleep disorders.
Clinicopathological Characteristics and Long-Term Outcomes of Cystic vs. Solid Pancreatic Neuroendocrine Tumors
Authors: Ventin Marco , Arya Shahrzad , Cattaneo Giulia , Fernandez-del Castillo Carlos , Wei Alice C. , Zureikat Amer H. , Alvikas Jurgis , Javed Ammar A. , Campbell Brady A. , Sekigami Yurie , Zheng Jian , Qadan Motaz , Lillemoe Keith D. , He Jin , Ferrone Cristina R. ,   the PAncreatic Neuroendocrine Disease Alliance (PANDA)
Published: January 2025

Objective:
To investigate the clinicopathological features and long-term outcomes of cystic and solid pancreatic neuroendocrine tumors (PanNETs).

Summary Background Data:
PanNETs uncommonly present as cystic lesions. Whether cystic PanNETs represent a distinct clinical entity compared to solid PanNETs is controversial.

Methods:
Clinicopathologic data of patients with resected PanNETs were collected from 4 high-volume centers between 2000-2019. Clinicopathological characteristics and outcomes of patients with cystic and solid PanNETs were compared based on a 3 cm tumor size cut-off using the Chi-squared test and Mann–Whitney U test. Survival estimates were calculated with the Kaplan-Meier method and log-rank test and multivariable analysis using a Cox proportional hazard model.

Results:
Of the 1727 patients undergoing pancreatectomy for PanNET, the median age was 58.1 years (IQR, 18.4), and 53.3% were male. Of these, 177 (10.3%) were cystic and 1550 (89.7%) solid. Cystic PanNETs were more prevalent in patients with hereditary syndromes, less frequently functional, and more often located in the body/tail of the pancreas. After the exclusion of patients with functional tumors, WHO G3 tumors, hereditary syndromes, neoadjuvant treatment, and metastatic stage, 145 cystic PanNETs were compared to 1059 solid PanNETs, and the median follow-up period of the cohort was 64 months. Cystic PanNETs demonstrated significantly fewer high-risk histopathological features, lymph node metastases (5.5% vs. 24.0%, P<0.001), and distant recurrence (4.1% vs. 14.4%; P<0.001). Among tumors ≤3 cm, cystic PanNETs had a low rate of lymph node metastases (3.9% vs. 17.8%; P<0.001), recurrence (3.1% vs. 8.4%; P=0.041), and low propensity to recur distantly. Cystic PanNETs had favorable long-term survival regardless of tumor size.

Conclusion:
Cystic PanNETs have a more benign course than their solid counterparts and conservative management can be considered for EUS-FNA-proven cystic PanNETs ≤3 cm. Parenchyma and lymph-node sparing resections are warranted in patients with cystic PanNETs>3 cm. Patients with poor baseline performance status may forego cystic PanNET resection and not affect their overall survival.
Fast and flexible minimizer digestion with digest
Authors: Zheng Alan , Lee Ishmeal , Shivakumar Vikram S. , Ahmed Omar Y. , Langmead Ben 
Published: January 2025

AbstractMinimizer digestion is an increasingly common component of bioinformatics tools, including tools for De Bruijn-Graph assembly and sequence classification. We describe a new open source tool and library to facilitate efficient digestion of genomic sequences. It can produce digests based on the related ideas of minimizers, modimizers or syncmers.Digestuses efficient data structures, scales well to many threads, and produces digests with expected spacings between digested elements.Digestis implemented in C++17 with a Python API, and is available open-source athttps://github.com/VeryAmazed/digest.