bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of selective serotonin reuptake inhibitor treatment following diagnosis of depression on suicidal behaviour risk: a target trial emulation
Authors: Lagerberg Tyra , Matthews Anthony A. , Zhu Nanbo , Fazel Seena , Carrero Juan-Jesus , Chang Zheng 
Published: July 2023

AbstractThere is concern regarding the impact of selective serotonin reuptake inhibitors (SSRIs) on suicidal behaviour. Using the target trial framework, we investigated the effect on suicidal behaviour of SSRI treatment following a depression diagnosis. We identified 162,267 individuals receiving a depression diagnosis aged 6–59 years during 2006–2018 in Stockholm County, Sweden, after at least 1 year without antidepressant dispensation. Individuals who initiated an SSRI within 28 days of the diagnosis were assigned as SSRI initiators, others as non-initiators. Intention-to-treat and per-protocol effects were estimated; for the latter, individuals were censored when they ceased adhering to their assigned treatment strategy. We applied inverse probability weighting (IPW) to account for baseline confounding in the intention-to-treat analysis, and additionally for treatment non-adherence and time-varying confounding in the per-protocol analysis. The suicidal behaviour risk difference (RD), and risk ratio (RR) between SSRI initiators and non-initiators were estimated at 12 weeks. In the overall cohort, we found an increased risk of suicidal behaviour among SSRI initiators (intention-to-treat RR = 1.50, 95% CI = 1.25, 1.80; per-protocol RR = 1.69, 95% CI = 1.20, 2.36). In age strata, we only found evidence of an increased risk among individuals under age 25, with the greatest risk among 6–17-year-olds (intention-to-treat RR = 2.90, 95% CI = 1.72, 4.91; per-protocol RR = 3.34, 95% CI = 1.59, 7.00). Our finding of an increased suicidal behaviour risk among individuals under age 25 reflects evidence from RCTs. We found no evidence of an effect in the high-risk group of individuals with past suicidal behaviour. Further studies with information on a wider array of confounders are called for.
IL4I1-catalyzed tryptophan metabolites mediate the anti-inflammatory function of cytokine-primed human muscle stem cells
Authors: Zuo Muqiu , Fang Jiankai , Huang Peiqing , Liu Shisong , Hou Pengbo , Wang Shiqing , Liu Zhanhong , Feng Chao , Cao Lijuan , Li Peishan , Shi Yufang , Shao Changshun 
Published: July 2023

AbstractMuscle stem cells (MuSCs) have been demonstrated to exert impressive therapeutic efficacy in disease settings through orchestrating inflammatory microenvironments. Nevertheless, the mechanisms underlying the immunoregulatory property of MuSCs remain largely uncharacterized. Here, we showed that interleukin-4-induced-1 (IL4I1), an essential enzyme that catalyzes indole metabolism in humans, was highly expressed in human MuSCs exposed to IFN-γ and TNF-α. Functionally, the MuSCs were found to inhibit the infiltration of neutrophils into sites of inflammation in a IL4I1-dependent manner and thus ameliorate acute lung injury in mice. Mechanistically, the indole metabolites, including indole-3-pyruvic acid (I3P) and indole-3-aldehyde (I3A), produced by IL4I1, acted as ligands to activate aryl hydrocarbon receptor (AHR), leading to augmented expression of TNF-stimulated gene 6 (TSG-6) in inflammatory cytokine-primed MuSCs. Furthermore, I3P administration alone suppressed neutrophil infiltration into damaged lungs. I3P could also reduce the level of reactive oxygen species in neutrophils. Therefore, our study has uncovered a novel mechanism by which MuSCs acquire their immunoregulatory property and may help to develop or optimize MuSC-based therapies for inflammatory diseases.
Two complementary genes in a presence-absence variation contribute to indica-japonica reproductive isolation in rice
Authors: Wang Daiqi , Wang Hongru , Xu Xiaomei , Wang Man , Wang Yahuan , Chen Hong , Ping Fei , Zhong Huanhuan , Mu Zhengkun , Xie Wantong , Li Xiangyu , Feng Jingbin , Zhang Milan , Fan Zhilan , Yang Tifeng , Zhao Junliang , Liu Bin , Ruan Ying , Zhang Guiquan , Liu Chunlin , Liu Ziqiang 
Published: July 2023

AbstractUnderstanding the evolutionary forces in speciation is a central goal in evolutionary biology. Asian cultivated rice has two subspecies, indica and japonica, but the underlying mechanism of the partial reproductive isolation between them remains obscure. Here we show a presence-absence variation (PAV) at the Se locus functions as an indica-japonica reproductive barrier by causing hybrid sterility (HS) in indica-japonica crosses. The locus comprises two adjacent genes: ORF3 encodes a sporophytic pollen killer, whereas ORF4 protects pollen in a gametophytic manner. In F1 of indica-japonica crosses, pollen with the japonica haplotype, which lacks the sequence containing the protective ORF4, is aborted due to the pollen-killing effect of ORF3 from indica. Evolutionary analysis suggests ORF3 is a gene associated with the Asian cultivated rice species complex, and the PAV has contributed to the reproductive isolation between the two subspecies of Asian cultivated rice. Our analyses provide perspectives on rice inter-subspecies post-zygotic isolation, and will promote efforts to overcome reproductive barriers in indica-japonica hybrid rice breeding.
Safety and immunogenicity of a SARS-CoV-2 Gamma variant RBD-based protein adjuvanted vaccine used as booster in healthy adults
Authors: Pasquevich Karina A. , Coria Lorena M. , Ceballos Ana , Mazzitelli Bianca , Rodriguez Juan Manuel , Demaría Agostina , Pueblas Castro Celeste , Bruno Laura , Saposnik Lucas , Salvatori Melina , Varese Augusto , González Soledad , González Martínez Veronica V. , Geffner Jorge , Álvarez Diego ,   Laboratorio Pablo Cassará R&D and CMC for ARVAC CG consortium, del Priore Sabrina A. , Hernando Insua Andrés C. , Kaufmann Ingrid G. , Di María Adrián , Góngora Adrián , Moreno Agustín , Cervellini Susana , Martin Blasco , Ali Esteban , Albarracín Romina , Bruno Barsanti , Toneguzzo Fernando , Sasso Guillermina , Stamer Sebastian , Cardoso Regina , Chajet Alejandro , Feleder Ethel , Halabe Karina , Perez Lera Pablo E. , de Oca Federico Montes , Vega Julio C. , Lombardo Mónica , Yerino Gustavo A. , Fló Juan , Cassataro Juliana 
Published: July 2023

AbstractA Gamma Variant RBD-based aluminum hydroxide adjuvanted vaccine called ARVAC CG was selected for a first in human clinical trial. Healthy male and female participants (18-55 years old) with a complete COVID-19-primary vaccine scheme were assigned to receive two intramuscular doses of either a low-dose or a high-dose of ARVAC CG. The primary endpoint was safety. The secondary objective was humoral immunogenicity. Cellular immune responses were studied as an exploratory objective. The trial was prospectively registered in PRIISA.BA (Registration Code 6564) and ANMAT and retrospectively registered in ClinicalTrials.gov (NCT05656508). Samples from participants of a surveillance strategy implemented by the Ministry of Health of the Province of Buenos Aires that were boosted with BNT162b2 were also analyzed to compare with the booster effect of ARVAC CG. ARVAC CG exhibits a satisfactory safety profile, a robust and broad booster response of neutralizing antibodies against the Ancestral strain of SARS-CoV-2 and the Gamma, Delta, Omicron BA.1 and Omicron BA.5 variants of concern and a booster effect on T cell immunity in individuals previously immunized with different COVID-19 vaccine platforms.
Thermo-electro-mechanical microstructural interdependences in conductive thermoplastics
Authors: Crespo-Miguel Javier , Lucarini Sergio , Arias Angel , Garcia-Gonzalez Daniel 
Published: July 2023

AbstractAdditive manufacturing has enabled the design of thermoplastic components that provide structural support, electrical conductivity and heat generation modulated by mechanical deformation. The mechanisms and interplays that govern the material response at the microstructural level remain, however, elusive. Here, we develop an experimental method to characterise conductive filaments from a combined mechanical, electrical and thermal perspective. This approach is used to unravel exciting material interplays of conductive polylactic acid. To overcome experimental limitations that prevent a complete microstructural analysis of the problem, we develop a full-field homogenisation framework and implement it for finite elements. The framework accounts for viscoplasticity, electrical and thermal conduction, convection and heat generation via Joule effect, as well as for the interdependences between them. After experimental validation, the framework is applied to virtually optimise fabrication requirements to obtain desired properties in final products, i.e., stiffer products, filaments with higher conductivities or with better sensing capabilities.
Annotated dataset for deep-learning-based bacterial colony detection
Authors: Makrai László , Fodróczy Bettina , Nagy Sára Ágnes , Czeiszing Péter , Csabai István , Szita Géza , Solymosi Norbert 
Published: July 2023

AbstractQuantifying bacteria per unit mass or volume is a common task in various fields of microbiology (e.g., infectiology and food hygiene). Most bacteria can be grown on culture media. The unicellular bacteria reproduce by dividing into two cells, which increases the number of bacteria in the population. Methodologically, this can be followed by culture procedures, which mostly involve determining the number of bacterial colonies on the solid culture media that are visible to the naked eye. However, it is a time-consuming and laborious professional activity. Addressing the automation of colony counting by convolutional neural networks in our work, we have cultured 24 bacteria species of veterinary importance with different concentrations on solid media. A total of 56,865 colonies were annotated manually by bounding boxes on the 369 digital images of bacterial cultures. The published dataset will help developments that use artificial intelligence to automate the counting of bacterial colonies.
Suppression of VEGF and inflammatory cytokines, modulation of Annexin A1 and organ functions by galloylquinic acids in breast cancer model
Authors: Abd El-Salam Mohamed , El-Tanbouly Ghada , Bastos Jairo , Metwaly Heba 
Published: July 2023

AbstractThe ongoing development of novel drugs for breast cancer aims to improve therapeutic outcomes, reduce toxicities, and mitigate resistance to chemotherapeutic agents. Doxorubicin (Dox) is known for its significant side effects caused by non-specific cytotoxicity. In this study, we investigated the antitumor activity of galloylquinic acids (BF) and the beneficial role of their combination with Dox in an Ehrlich ascites carcinoma (EAC)-bearing mouse model, as well as their cytotoxic effect on MCF-7 cells. The EAC-mice were randomized into five experimental groups: normal saline, Dox (2 mg/kg, i.p), BF (150 mg/kg, orally), Dox and BF combined mixture, and a control group. Mice were subjected to a 14-day treatment regimen. Results showed that BF compounds exerted chemopreventive effects in EAC mice group by increasing mean survival time, decreasing tumor volume, inhibiting ascites tumor cell count, modulating body weight changes, and preventing multi-organ histopathological alterations. BF suppressed the increased levels of inflammatory mediators (IL-6 and TNF-α) and the angiogenic marker VEGF in the ascitic fluid. In addition, BF and their combination with Dox exhibited significant cytotoxic activity on MCF-7 cells by inhibiting cell viability and modulating Annexin A1 level. Moreover, BF treatments could revert oxidative stress, restore liver and kidney functions, and normalize blood cell counts.
The association between access to key household resources and violence against women
Authors: Bandyopadhyay Siddhartha , Sarkar Sanjukta , Sensarma Rudra 
Published: July 2023

AbstractWe provide the first comprehensive analysis of the association between two key household resources (drinking water and toilet access) and both non-partner violence (NPV) and intimate partner violence (IPV) experienced by women. We use data from a nationally representative household survey for India obtained from the latest (fourth) round of the National Family Health Survey conducted in 2015–16. We employ logistic regression method and also use inverse-probability-weighted regression adjustment to control for selection bias. We find that NPV decreases with access to drinking water, while IPV decreases with provision of toilets. These results are found to be robust to an alternative method viz. propensity score matching and selection on unobservables using the Rosenbaum bounds approach.
Distribution characteristics of organic carbon (nitrogen) content, cation exchange capacity, and specific surface area in different soil particle sizes
Authors: Bi Xiaoqian , Chu Hang , Fu Mingming , Xu Dandan , Zhao Wenyu , Zhong Yijian , Wang Mei , Li Ke , Zhang Ya-nan 
Published: July 2023

AbstractUnderstanding the distribution of soil organic carbon and nitrogen (OC(N)) content, cation exchange capacity (CEC), and specific surface area (SSA) in different soil particle sizes is crucial for studying soil fertility and properties. In this study, we investigated the distribution characteristics of the OC(N), CECand SSA in different particles of yellow–brown soil under different methods. The result revealed that as the particle size decreased, the soil OC(N), SSA and CEC content gradually increase. The content of OC and ON different soil particles ranged from 1.50–28.16 g·kg−1 to 0.18–3.78 g·kg−1, respectively, and exhibited significant differences between different particles. We observed good linear relationships between OC and ON in different particle sizes of yellow–brown soil under different utilization methods, with correlation coefficients ranging from 0.86 to 0.98, reaching a very significant level (n = 12, p < 0.01). The ranges of SSA and CEC in different particles of the four soils were 0.30–94.70 m2·g−1 and 0.70–62.91 cmol·kg−1, respectively. Additionally, we found logarithmic relationships between SSA (CEC) and the equivalent diameter for the four soils, with correlation coefficients (r2) higher than 0.91. Furthermore, there was an extremely significant linear relationship between CEC and SSA of the four soils, with correlation coefficients (r2) of 0.92–0.97 (n = 12, p < 0.01). These results highlight the close relationship between soil particle size and soil OC(N), SSA, and CEC. The conclusions drawn from this study provide valuable data support and a theoretical basis for further understanding soil properties.
Differential exon usage of developmental genes is associated with deregulated epigenetic marks
Authors: Do Hoang Thu Trang , Shanak Siba , Barghash Ahmad , Helms Volkhard 
Published: July 2023

AbstractAlternative exon usage is known to affect a large portion of genes in mammalian genomes. Importantly, different splice isoforms sometimes possess distinctly different protein functions. Here, we analyzed data from the Human Epigenome Atlas for 11 different human adult tissues and for 8 cultured cells that mimic early developmental stages. We found a significant enrichment of cases where differential usage of exons in various developmental stages of human cells and tissues is associated with differential epigenetic modifications in the flanking regions of individual exons. Many of the genes that were differentially regulated at the exon level and showed deregulated histone marks at the respective exon flanks are functionally associated with development and metabolism.
Full thickness 3D in vitro conjunctiva model enables goblet cell differentiation
Authors: Schwebler Julian , Fey Christina , Kampik Daniel , Lotz Christian 
Published: July 2023

AbstractIn vitro culture and generation of highly specialized goblet cells is still a major challenge in conjunctival 3D in vitro equivalents. A model comprising all physiological factors, including mucus-secreting goblet cells has the potential to act as a new platform for studies on conjunctival diseases. We isolated primary conjunctival epithelial cells and fibroblasts from human biopsies. 3D models were generated from either epithelial layers or a combination of those with a connective tissue equivalent. Epithelial models were investigated for marker expression and barrier function. Full-thickness models were analyzed for goblet cell morphology and marker expression via immunofluorescence and quantitative real-time PCR. Simple epithelial models cultured at the air–liquid interface showed stratified multi-layer epithelia with pathologic keratinization and without goblet cell formation. The combination with a connective tissue equivalent to generate a full-thickness model led to the formation of a non-keratinized stratified multi-layer epithelium and induced goblet cell differentiation. In our model, a high resemblance to natural conjunctiva was achieved by the combination of conjunctival epithelial cells with fibroblasts embedded in a collagen-hydrogel as connective tissue equivalent. In the future, our conjunctival in vitro equivalent enables the investigation of goblet cell differentiation, conjunctival pathologies as well as drug testing.
Comparison between the geological features of Venus and Earth based on gravity aspects
Authors: Karimi Kurosh , Kletetschka Gunther , Meier Verena 
Published: July 2023

AbstractWe probe the gravitational properties of two neighboring planets, Earth and Venus. To justify a comparison between gravity models of the two planets, spherical harmonic series were considered up to a degree and order of 100. The topography and gravity aspects, including $${\Gamma }_{zz}$$

Γ

zz

(vertical derivative of the vertical component of the gravity field), strike alignment (SA), comb factor (CF), and I2 invariant derived from the Marussi tensor, were calculated for the two planets at specifically selected zones that provided sufficient resolution. From Γzz we discovered that the N-NW edge of Lakshmi Planum does not show any subduction-like features. Its Γzz signature resembles passive continental margins on Earth, like those surrounding the Indian Peninsula. Moreover, according to SA and CF, the Pacific and Philippine-North American Contact Zone on Earth indicates significantly higher level of deformation due to convergent motion of the plates, whereas the deformation level on Venus is significantly smaller and local, when considering an equatorial rifting zone (ERZ) of Venus (between Atla-Beta Regios) as diverging boundaries. The strain mode on the East African Rift system is smaller in comparison with ERZ as its Venusian analog. The topography-I2 analysis suggests a complicated nature of the topographic rise on Beta Regio. We show that specific regions in this volcanic rise are in incipient stages of upward motion, with denser mantle material approaching the surface and thinning the crust, whereas some risen districts show molten and less dense underlying crustal materials. Other elevated districts appear to be due to mantle plumes and local volcanic activities with large density of underlying material.
Computational modeling and synthesis of pyridine variants of benzoyl-phenoxy-acetamide with high glioblastoma cytotoxicity and brain tumor penetration
Authors: Ingraham Charles H. , Stalinska Joanna , Carson Sean C. , Colley Susan B. , Rak Monika , Lassak Adam , Peruzzi Francesca , Reiss Krzysztof , Jursic Branko S. 
Published: July 2023

AbstractGlioblastomas are highly aggressive brain tumors for which therapeutic options are very limited. In a quest for new anti-glioblastoma drugs, we focused on specific structural modifications to the benzoyl-phenoxy-acetamide (BPA) structure present in a common lipid-lowering drug, fenofibrate, and in our first prototype glioblastoma drug, PP1. Here, we propose extensive computational analyses to improve the selection of the most effective glioblastoma drug candidates. Initially, over 100 structural BPA variations were analyzed and their physicochemical properties, such as water solubility (− logS), calculated partition coefficient (ClogP), probability for BBB crossing (BBB_SCORE), probability for CNS penetration (CNS-MPO) and calculated cardiotoxicity (hERG), were evaluated. This integrated approach allowed us to select pyridine variants of BPA that show improved BBB penetration, water solubility, and low cardiotoxicity. Herein the top 24 compounds were synthesized and analyzed in cell culture. Six of them demonstrated glioblastoma toxicity with IC50 ranging from 0.59 to 3.24 µM. Importantly, one of the compounds, HR68, accumulated in the brain tumor tissue at 3.7 ± 0.5 µM, which exceeds its glioblastoma IC50 (1.17 µM) by over threefold.
Automatic electrode scalar location assessment after cochlear implantation using a novel imaging software
Authors: Geiger S. , Iso-Mustajärvi M. , Nauwelaers T. , Avci E. , Julkunen P. , Linder P. , Silvast T. , Dietz A. 
Published: July 2023

AbstractAs of today, image-based assessment of cochlear implant electrode array location is not part of the clinical routine. Low resolution and contrast of computer tomography (CT) imaging, as well as electrode array artefacts, prevent visibility of intracochlear structures and result in low accuracy in determining location of the electrode array. Further, trauma assessment based on clinical-CT images requires a uniform image-based trauma scaling. Goal of this study was to evaluate the accuracy of a novel imaging software to detect electrode scalar location. Six cadaveric temporal bones were implanted with Advanced Bionics SlimJ and Mid-Scala electrode arrays. Clinical-CT scans were taken pre- and postoperatively. In addition, micro-CTs were taken post-operatively for validation. The electrode scalar location rating done by the software was compared to the rating of two experienced otosurgeons and the micro-CT images. A 3-step electrode scalar location grading scale (0 = electrode in scala tympani, 1 = interaction of electrode with basilar membrane/osseous spiral lamina, 2 = translocation of electrode into scala vestibuli) was introduced for the assessment. The software showed a high sensitivity of 100% and a specificity of 98.7% for rating the electrode location. The correlation between rating methods was strong (kappa > 0.890). The software gives a fast and reliable method of evaluating electrode scalar location for cone beam CT scans. The introduced electrode location grading scale was adapted for assessing clinical CT images.
Quality evaluation of water disclosure of Chinese papermaking enterprises based on accelerated genetic algorithm
Authors: He Chaoran , Shen Juqin , Xu Jiawei , Sun Fuhua , Wang Bing 
Published: July 2023

AbstractAs the carrier of enterprise water resources management disclosure, water information disclosure is a means of expression of enterprises’ environmental responsibility. First, a corporate water information disclosure quality evaluation index system and evaluation method are established, and with the help of the projection tracing method of accelerated genetic algorithm, 27 paper companies in China are selected as a sample and the disclosure quality level is analyzed empirically. Then, the analysis is carried out in terms of three changes in vertical trends, horizontal trends and changes in laws, regulations and policies, and the results show that Chinese paper and paper product enterprises have low quality of water information disclosure, weak disclosure content and low voluntary disclosure. Finally, feasible suggestions are made based on the evaluation of disclosure issues.