bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological function research of the long non-coding RNA Malnc2310 in bananas induced by Fusarium oxysporum f. sp. cubense
Authors: Li Wenbin , Zhao Pingjuan , Sun Jianbo , Yu Xiaoling , Zou Liangping , Li Shuxia , Di Rong , Ruan Mengbin , Peng Ming 
Published: December 2022

Abstract
Long non-coding RNAs (lncRNAs) participate in the biological processes of plants under biotic and abiotic stresses. However, little is known about the function and regulation mechanism of the pathogen-related lncRNAs at a molecular level. A banana lncRNA, namely Malnc2310, is greatly induced by Fusarium oxysporum f. sp. cubense TR4 strain (VCG01213/16) in roots. In this study, we indicate the nuclear localization of Malnc2310 by fluorescence in situ hybridization (FISH). We illustrate that Malnc2310 interacts with several proteins that are related to phenylpropanoid pathway, pathogen response and programmed cell death. Overexpression of Malnc2310 confers higher susceptibility to stresses, such as Fusarium crude extracts, salinity and cold in transgenic Arabidopsis. Furthermore, the Malnc2310 transgenic Arabidopsis accumulated more anthocyanin under Fusarium crude extracts and cold treatments. The Malnc2310 overexpression up-regulated the expression of several key genes that are related to the development of lateral roots and those involved in the anthocyanin biosynthesis pathway. Malnc2310 also enhanced the activity of phenylalanine ammonia lyase (PAL) in transgenic Arabidopsis. Based on our findings, we propose that Malnc2310 binds to PAL to participate in the flavonoid metabolism under stresses. This study provides new insights into the role of Malnc2310 in mediating plants to stress adaptation.
Relationship between Atmosphere of Psychiatric Wards and Motivation, Attitude and Perception of Patients Hospitalized in Shiraz and Bushehr University Hospitals - 2019
Authors: MA Abolhoseini , Bahreini M , Sh Pouladi , Bagherzadeh R 
Published: December 2022

Abstract
Research Background: Treatment of psychiatric patients is a challenging care issue that requires special attention due to the special nature and conditions of these patients. Detecting the factors affecting the acceptance of treatment by these patients can help control the disease and promote their health. The environment where people live can affect their behaviors. The present study aimed to determine the relationship between ward atmosphere with motivation, perception, and attitude of patients towards continuing the treatment process in psychiatric wards of certain university hospitals in Shiraz and Bushehr in 2019.
Methods: The present cross-sectional study included 299 patients admitted to the psychiatric wards of Bushehr and Shiraz hospitals. A convenience sampling method was performed. Data collection tools included demographic characteristics form, a short-form psychiatric ward questionnaire, and patient attitude, motivation, and treatment perception questionnaires. Data were analyzed using SPSS 19 and Pearson or Spearman correlation tests, independent t-test, one-way analysis of variance, and linear regression. The significance level was considered less than 0.05 in all cases.
Results: The mean score of ward atmosphere was lower than average (18.37±2.99) from the patients' point of view. Among the subscales of ward atmosphere from the patients' point of view, the "support" subscale was a predictor of attitude towards continuing the treatment process (P=0.042, β=-0.131). The negative attitude toward treatment was lower at higher levels of support. Two subscales, namely "support" (P=0.048, β=0.120) and "programme clarity" (P<0.001, β = 0.221) were predictors of patients' perception of the treatment continuation; in other words, the patients' perception of the treatment process was higher at higher levels of these two subscales. None of the ward atmosphere subscales were related to the patient's motivation to continue the treatment process from the patient's point of view. (P>0.05(
Conclusion: Given the relationship between at least some sub-scales of ward atmosphere and patients' attitudes and perceptions about continuing the treatment process, designing ward atmosphere optimization programs is recommended to improve patients' attitudes and perceptions to continue the treatment process. It is recommended to study other factors affecting treatment in psychiatric patients.
Serum metabolome signatures characterizing co-infection of Plasmodium falciparum and HBV in pregnant women
Authors: Asantewaa Gloria , Anabire Nsoh Godwin , Bauer Michael , Weis Sebastien , Neugebauer Sophie , Quaye Osbourne , Helegbe Gideon Kofi 
Published: December 2022

Abstract
Background
Pregnant women are among the most susceptible groups to infectious diseases. In Northern Ghana, Plasmodium falciparum (P. falciparum) and hepatitis B virus (HBV) co-infection is prevalent among pregnant women. and has been found to induce an augmented inflammatory immune response and aggravate liver damage.
Objective
Mono-infection with either of these pathogens results in unique metabolic alterations. Thus, we aimed to explicate the effects of co-infection of these two pathogens on the metabolome signatures of pregnant women. This could give an indication to the metabolic pathways that are impacted and provide useful prognostic or diagnostic markers.
Methods
Using MS/MS-based targeted metabolomic approach, we determined the serum metabolome in pregnant women with either P. falciparum mono-infection, HBV mono-infection, P. falciparum and HBV co-infection or in un-infected (control) women.
Results
We observed that concentrations of sphingolipids were significantly decreased in subjects with P. falciparum mono-infection. Whereas amino acids and phospholipids were decreased in subjects with HBV mono-infection. Co-infection of the two pathogens was found to be characterized distinctively by reduced concentrations of phospholipids and hexoses (mostly glucose). Additionally, co-infection was characterized by altered pathways that contribute to redox homeostasis. Overall, PC ae C40:1 was found to be a good discriminatory metabolite for the co-infection group.
Conclusions
Put together, the metabolite PC ae C40:1, could serve as a potential biomarker for characterizing the co-infection, and can further be explored for use in diagnosis and treatment of malaria and chronic hepatitis B co-morbidity as well as distinguishing co-infections from cases of mono-infections.
Construction of a vascularized cardiopulmonary resuscitation mannequin with hemodynamic monitoring including peripheral vasculature
Authors: Bagha Imran , Sarvas Holly , Ropp Scott , Peters Samuel 
Published: December 2022

AbstractIntroduction: Feedback devices for cardiopulmonary resuscitation (CPR) currently register compression rate, depth, recoil and land marking. There remains a gap in determining the impact of peripheral vascularization, blood pressure, and blood flow as a result of quality CPR compressions.Methods: Our team designed a closed-loop CPR mannequin model that represented the vascularization of a human, including peripheral lower limbs. A disposable, ultrasound bandage (Flosonics Flopatch™) was applied to measure the blood flow. The model consisted of a CPR mannequin and feedback software, pressure monitoring device, patient monitor, Polyvinyl chloride (PVC) tubing and connectors, siphon bulb, 3D printed parts and wood for stabilization, Kelly clamps, and water mixture to replicate blood. A full cost breakdown and set-up is provided.Results: 28 Basic Life Saving-trained individuals tested the device both clamped and unclamped to peripheral vasculature. CPR was performed for 5 minutes at 60bpm to mimic human heart rate with siphon bulb limitations. Findings demonstrate that pulse pressure mean was 69.9mmHg clamped and 65.0mmHg unclamped (p = 0.03), consistent with expected values anticipated during effective compressions. Blood flow velocity was statistically insignificant, and cannot be inferred on due to inconsistencies with the ultrasound bandage.Conclusions:The CPR vascularization prototype was effective in replicating blood pressures of a human adult circulatory system, including peripheral vasculature. There remains limitations to state the model was effective for replicating blood flow velocity with the Flopatch™, further testing is required. The use of Kelly clamps was effective in restricting blood flow to tube sections.
Exploring the biopsychosocial impact of hypermobility spectrum disorders (HSD) and Ehlers-Danlos syndrome (EDS) in an adult population: a protocol for a scoping review
Authors: Clark Natalie Louise , Johnson Melissa , Rangan Amar , Swainston Katherine , Kottam Lucksy 
Published: December 2022

Abstract
Background
Conditions such as hypermobility spectrum disorders (HSD) and Ehlers-Danlos Syndrome (EDS), are most often diagnosed when an individual has joint flexibility beyond the normal physiological limits. Additional characteristics and symptoms include pain and fatigue with individuals also being more likely to report feelings of anxiety and depression. Due to the varied presentation of these conditions, there is a lack of understanding amongst the various healthcare professionals’ (HCPs) individuals present to, leading to delayed diagnoses and negative experiences for the individuals themselves. This scoping review therefore aims to map the known biopsychosocial impact of adults with HSD and EDS.
Methods
The scoping review will follow the six-step framework as outlined by Arskey and O’Malley and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses for Scoping Reviews (PRISMA-ScR) checklist. The search will be conducted using the following databases: AMED, CINAHL, Cochrane Library, EMBASE, MEDLINE, PsycINFO, PubMed, PEDro. Full text published articles in the English language (excluding literature and systematic reviews) with adult samples (over the age of 18 years) and a diagnosis of a HSD or EDS, published between 2012 and 2022 will be included in the review.
Discussion
This review will aim to explore the existing literature for the reported biopsychosocial impact of adults with a HSD or EDS. It will also aim to further acknowledge the gaps in understanding of the condition, how the condition and the impact of the condition is being measured, as well as what HCPs are involved in supporting such individuals. These gaps will be used to inform a future systematic review. It is the overall goal to increase the knowledge of HCPs and the quality of life of adults living with a joint hypermobility condition.
Feeding hempseed cake alters the bovine gut, respiratory and reproductive microbiota
Authors: Winders Thomas , Holman Devin , Schmidt Kaycie , Luecke Sarah , Smith David , Neville Bryan , Dahlen Carl , Swanson Kendall , Amat Samat 
Published: December 2022

Abstract
A growing number of studies have investigated the feasibility of utilizing hemp by-products as livestock feedstuffs; however, their impact on livestock microbiomes remains unexplored. Here, we evaluated the effects of feeding hempseed cake on the gastrointestinal, respiratory, and reproductive microbiota in beef heifers. Angus-crossbred heifers (19-months old) were fed a corn-based finishing diet containing 20% hempseed cake as a substitute for 20% corn dried distillers’ grains with solubles (DM basis; Control; n = 16/group) for 111 days until slaughter. Ruminal fluid and deep nasopharyngeal swabs (days 0, 7, 42, 70 and 98), and vaginal and uterine swabs (at slaughter) were collected, and the microbiota assessed using 16S rRNA gene sequencing. Diet affected the community structure of the ruminal (d7– 98; 0.06 ≤ R2 ≤ 0.12; P < 0.05), nasopharyngeal (d 98; R2 = 0.18; P < 0.001), and vaginal (R2 = 0.06; P < 0.01) microbiota. Heifers fed hempseed cake had increased microbial diversity in the rumen, reduced microbial richness in the vagina, and greater microbial diversity and richness in the uterus. In addition to the distinct microbial communities in the rumen, nasopharynx, vagina and uterus, we identified 28 core taxa that were shared (≥ 60% of all samples) across these sampling locations. Feeding hempseed cake appeared to alter the bovine gut, respiratory and reproductive microbiota. Our findings also highlight the need for research evaluating the impact of hemp-associated food and personal care products on the human microbiome.
Data-Driven Profiles of Attention-Deficit/Hyperactivity Disorder Using Objective and Ecological Measures of Attention, Distractibility and Hyperactivity.
Authors: Fernández-Martín Pilar , Rodríguez-Herrera Rocio , López Rosa Cánovas , Díaz-Orueta Unai , Arboleas Alma Martínez de Salazar , Cubos Pilar Flores 
Published: December 2022

Abstract

In the past two decades, the traditional nosology of Attention-Deficit/Hyperactivity Disorder (ADHD) has been criticized for having insufficient discriminant validity. As an alternative, dimensional frameworks to psychopathology pursue disentangling within-diagnostic heterogeneity and define more reliable and clinically useful nosologies. In line with this trend, in this study, we adopted a data-driven approach to ecological and objective measures of attentional control, impulsivity and hyperactivity, with the aim of identifying clinically useful profiles of ADHD. 110 Spanish-speaking participants (6–16 years) with ADHD (medication-naïve.n = 57) and typically developing (n = 53) completed AULA, a virtual-reality continuous performance test. We first examined AULA performance using DSM-5 diagnosis and found a similar performance profile between ADHD subtypes. Then, we applied hybrid hierarchical k-means clustering algorithms to AULA’s main outcome measures. A five-cluster structure was the most optimal solution. We identified two ADHD phenotypes sharing attention impairments and hyperactivity but with opposing performance profiles on processing speed (PS) and response inhibition; two groups with average and high performance; and one average-performing group with poor sustained attention and slow PS. DSM-5 subtypes cut across cluster profiles. Our findings might suggest that PS and response inhibition, but not attentional processes and gross-motor activity, are useful domains to distinguish between ADHD subpopulations and understand mechanisms underlying attentional impairments. This study highlights the poor feasibility of categorical systems to parse ADHD heterogeneity and the added value of data-driven approaches and VR-based assessments to obtain an objective and less biased characterization of cognitive functioning in individuals with and without ADHD.
Efficacy and safety of mycophenolate mofetil for steroid reduction in neuromyelitis optica spectrum disorder: a prospective cohort study
Authors: Akatani Ritsu , Chihara Norio , Koto Shusuke , Mori Sotaro , Kurimoto Takuji , Nakamura Makoto , Tachibana Hisatsugu , Otsuka Yoshihisa , Ueda Takehiro , Omori Takashi , Sekiguchi Kenji , Matsumoto Riki 
Published: December 2022

Abstract

Background and Objective: Neuromyelitis optica spectrum disorder (NMOSD) is a rare autoimmune inflammatory disease of the central nervous system that affects multiple generations. Long-term immunosuppressive treatments, especially with prednisolone, for middle-aged and older patients with NMOSD can lead to various complications. In this study, we aimed to evaluate the efficacy of mycophenolate mofetil (MMF) in NMOSD relapse prevention and its safety when reducing oral prednisolone over time.
Methods: Patients with NMOSD receiving oral prednisolone were included. MMF was administrated with prednisolone dose reduction over 48 weeks. The primary endpoint was the achievement of a prednisolone dose reduction without relapse during MMF administration.
Results: Nine patients with NMOSD were enrolled, with a mean age of 56.4 years (standard deviation of 8.92). All patients were women, with a mean annualized relapse rate (ARR) of 0.72 during the last 2 years. The primary endpoint was achieved in seven patients (77.8% [95% confidence interval: 40–97%]; p < 0.0001, one-sample proportion test for exceeding the 10% threshold), whereas two patients relapsed during the treatment period. The mean ARR of all patients decreased from 0.72 to 0.22 (p < 0.05, paired t-test). Ten adverse events were reported during the observation period, all of which were mild (all Grade 1 according to the Common Terminology Criteria for Adverse Events).
Conclusions: MMF is effective for NMOSD and may be a treatment option for middle-aged and older patients who may benefit from steroid reduction.
Clinical trial registration number: jRCT, jRCTs051180080. Registered Feb. 27th, 2019-retrospectively registered, https://jrct.niph.go.jp/en-latest-detail/jRCTs051180080
Live donor nephrectomy side: Is it implicated in urologic complications among lady donors during their post-donation pregnancies?
Authors: Donia Ahmed Farouk , Elrefaie Eman , Refaie Ayman Fathi , Ghoneim Moatasem Elsayed , Osman Yasser , Ali-El-Dein Bedeir 
Published: December 2022

Abstract
Introduction:
Our center policy is to promote right nephrectomy for pre-menopausal live donor ladies. This is based on the traditional belief that urologic complications of post-donation pregnancies would be more frequent among lady donors with a solitary right (compared to left) kidney. Studies that support or dismiss our policy are lacking. Therefore, we conducted this study.
Methods:
100 ladies who had post-donation pregnancy were included. They underwent an updated clinical, laboratory and ultrasound assessment. They were classified into two groups: right and left nephrectomy groups. Both groups were compared relative to pre- and post-donation data as well as urologic complications during or after post-donation pregnancies and current kidney function.
Results:
Right nephrectomy was carried-out for 60 donors (60%). Post-donation acute pyelonephritis was not reported in either group. Unexpectedly, lower urinary tract infection during post-donation pregnancy occurred among 16.7% of ladies of the right nephrectomy group compared to 12.5% in the left nephrectomy group (p: non-significant).
Conclusion:
Despite that the endeavor to retrieve the right rather than the left kidney among lady donors could give them the benefit of doubt in regard to possible urological complications during their subsequent pregnancies, this policy is likely an overdoing practice. Larger-scale studies are needed.
Cx43 promotes exocytosis of damaged lysosomes through actin remodelling
Authors: Domingues Neuza , Catarino Steve , Cristovao Beatriz , Rodrigues Lisa , Filomena Carvalho , Sarmento Maria Joao , Zuzarte Monica , Almeida Jani , Fernandes Fabio , Rodrigues-Santos Paulo , Santos Nuno , Korolchuk Viktor , Gonçalves Teresa , Milosevic Ira , Raimundo Nuno , Girao Henrique 
Published: December 2022

Abstract
A robust cellular response to lysosomal membrane damage is essential to prevent lysosomal content leakage to the cytoplasm and subsequent activation of cell death pathways. Here we report exocytosis as an important response mechanism to lysosomal damage, which is further potentiated when membrane repair or lysosomal degradation mechanisms are impaired. Our data reveal that Connexin43 (Cx43), a protein canonically associated with gap junctions, is recruited to damaged lysosomes to promote their secretion, thereby accelerating cell recovery. The exocytotic effects were found to be dependent on actin reorganization: Cx43 expression was associated with actin network remodelling, increased plasma membrane fluidity and decreased cell stiffness. Furthermore, we demonstrate that Cx43 interacts with the actin nucleator Arp2, the activity of which was shown to be necessary for Cx43-mediated actin rearrangement and lysosomal exocytosis following damage. These results identify a novel mechanism of lysosomal quality control whereby Cx43-mediated actin remodelling potentiates the secretion of damaged lysosomes.
A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases
Authors: Batth Tanveer , Simonsen Jonas , Hernández-Rollán Cristina , Brander Søren , Morth Jens Preben , Johansen Katja , Nørholm Morten , Hoof Jakob , Olsen Jesper 
Published: December 2022

Abstract
Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes that help break down lignocellulose, making them highly attractive for improving biomass utilization in biotechnological purposes. The catalytically essential N-terminal histidine (His1) of LPMOs is post-translationally modified by methylation in filamentous fungi to protect them from auto-oxidative inactivation, however, the responsible methyltransferase enzyme is unknown. Using mass-spectrometry-based quantitative proteomics in combination with systematic CRISPR/Cas9 knockout screening in Aspergillus nidulans, we identified the N-terminal histidine methyltransferase (NHMT) encoded by the gene AN4663. Targeted proteomics confirmed that NHMT was solely responsible for His1 methylation of LPMOs. NHMT is predicted to encode a unique seven-transmembrane segment anchoring a soluble methyltransferase domain. Co-localization studies showed endoplasmic reticulum residence of NHMT and co-expression in the industrial production yeast Komagataella phaffii with LPMOs resulted in His1 methylation of the LPMOs. This demonstrates the biotechnological potential of recombinant production of proteins and peptides harbouring this unique post-translational modification.
The sensitivity of the zebrafish embryo coiling assay for the detection of neurotoxicity by compounds with diverse modes-of-action
Authors: von Hellfeld Rebecca , Gade Christoph , Baumann Lisa , Leist Marcel , Braunbeck Thomas 
Published: December 2022

Abstract
In the aim to determine neurotoxicity, new methods are being validated, including tests and test batteries comprising in vitro and in vivo approaches. Alternative test models such as the zebrafish (Danio rerio) embryo have received increasing attention, with minor modifications of the fish embryo toxicity test (FET; OECD TG 236) as a tool to assess behavioral endpoints related to neurotoxicity during early developmental stages. The spontaneous tail movement assay, also known as coiling assay, assesses the development of random movement into complex behavioral patterns and has proven sensitive to acetylcholine esterase inhibitors at sublethal concentrations. The present study explored the sensitivity of the assay to neurotoxicants with other modes-of-action. Here, five compounds with diverse modes-of-action were tested at sublethal concentrations: acrylamide, carbaryl, hexachlorophene, ibuprofen, and rotenone. While carbaryl, hexachlorophene, and rotenone consistently induced severe behavioral alterations by ~30 hours post fertilization (hpf), acrylamide and ibuprofen expressed time- and/or concentration-dependent effects. At 37 - 38 hpf, additional observations revealed behavioral changes during dark phases with a strict concentration-dependency. The study documented the applicability of the coiling assay to mode-of-action-dependent behavioral alterations at sublethal concentrations, underlining its potential as a component of a neurotoxicity test battery.