bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Use of Angelica Sinensis and β-tricalcium Phosphate in the Management of Surgically Induced Critical-sized Class II Furcation Defects in Dogs
Authors: Eldeeb Dina W. , Hommos Ahmed M. , Taalab Maha R. , Rehim Samia S. Abd El 
Published: December 2022

Abstract
Objective: Evaluating the effectiveness of Angelica sinensis, a widely used traditional Chinese medicine, in conjunction with Beta-tricalcium phosphate (β-TCP) bone graft in the management of induced critical sized class II furcation defects in adult healthy mongrel dogs.
Materials and Methods: A randomized study design was conducted on the third and fourth premolars of six healthy mongrel dogs. A total of twenty-four class II furcation critical-sized defects were surgically created. After reflecting a fully-reflected mucoperiosteal flap, twelve defects were filled with Angelica sinensis powder mixed with Beta-tricalcium phosphate (β-TCP), then collagen membranes were applied over the defects (experimental group). The other twelve defects were filled with β-TCP followed by collagen membrane application (control group). The dogs were sacrificed at the end of fourth and eighth weeks. Jaw segments containing the operated teeth were dissected and processed for histological examination and morphometric analysis of the regenerative features.
Results: The histological results revealed that bone formation and regeneration of cementum and PDL progressed more in experimental groups than in control groups. Moreover, the regenerative features were more advanced in the second observation period. The histomorphometry revealed that the surface area of newly formed bone and the thickness of its trabeculae were higher in the defects treated with Angelica sinensis and β-TCP than in only β-TCP-filled defects at both observational periods.
Conclusions: Angelica sinensis has accelerated the regenerative potential of the interradicular bone and has enhanced the effect of β-TCP for interradicular bone regeneration in dogs.
Application of constrained implants hip arthroplasty in patients with hip neuromuscular disease
Authors: Zhang Yanfang , Liu Zeming , Liu Sikai , Li Mengnan , Liu Bo , Han Yongtai , Wu Tao 
Published: December 2022

Abstract
Background: It is well known that neuromuscular diseases increase the risk of complications after hip replacement.The purpose of this study was to investigate the clinical manifestation and survival rate of total hip arthroplasty (THA) with constrained implants in inpatients with neuromuscular diseases.
Method: A retrospective study evaluated the results of 17 THA in 17 patients with hip neuromuscular disease from April 2018 to June 2020. The average age at the time of operation was 66.71 years (33-86 years). The average follow-up time was 34.44 months (25-50 months). All surgery were operated through posterolateral approach. These were cementless implants.
Result: The incisions of all cases healed in one stage, without infection, injury of important nerves, blood vessels and other tissues, loosening of joint prosthesis and deep venous thrombosis of lower limbs. One of the patients developed a new cerebral infarction at postoperative, which affected the contralateral limb movement and seriously affect the postoperative efficacy observation. The others were followed up for 25 months to 50 months, with an average of (34.44±8.05) months. All patients underwent THA with constrained implants. The hip joint function was evaluated according to Harris Hip score standard, excellent in 2 cases, good in 12 cases and poor in 2 cases.
Conclusion: THA combined with constrained implants has been shown to be effective in reducing the risk of dislocation and enhancing stability for patients with neuromuscular disease.
Green Synthesis of Copper Oxide Nanoparticles Using Leaf Extract of Justicia Schimperiana and their Antibacterial Activity
Authors: Melkamu Walelign Wubet , Feleke Eskedar Getachew 
Published: December 2022

Abstract
A fast, efficient, simple and environmentally-friendly, green synthesis method was employed for the synthesis of copper oxide nanoparticles (CuONPs) using easily available, low-cost and non-toxic Justicia Schimperiana plant leaf extract (JSPLE) from Ethiopia. Ultraviolet-Visible (UV-Vis) spectrophotometer, X-ray Diffraction (XRD) technique and Fourier-Transform Infrared (FTIR) Spectroscopy were used to characterize the synthesized CuONPs. The UV-Vis confirmed the formation of CuONPs and the maximum absorbance for the synthesized CuONPs in solution at 212 nm. The different parameters like penta hydrated copper sulphate concentration, volume of leaf extract, temperature, pH and time were optimized spectrophotometrically. The FTIR analysis confirmed the existence of various functional groups responsible for reducing and stabilizing during the biosynthesis process. The XRD result confirmed that the synthesized CuONPs were crystalline in nature with the monoclinic structure and the average crystallite sizes of the particles were 21.8 nm. The synthesized CuONPs showed antimicrobial (gram-negative bacteria (Salmonella typhimurium,Escherichia coli and Klebsiella pneumoniae) and grampositive bacteria (Staphylococcus aureus)) activity. It has been noted that the green synthesized CuONPs showed efficient antibacterial activity against gram-negative (salmonella typhimurium) bacteria with maximum ZOI of 18.6 mm with respect to the highest concentration (150 μg/mL). The developed method for the CuONPs synthesis using JSPLE is an eco-friendly and convenient method. In the near future, the synthesized CuONPs could be used in the fields of biomedicine, biosensor, water treatment and nanotechnology.
Evaluation of the implementation of hospital hygiene components in 30 health-care facilities in the Autonomous District of Abidjan (Cote d'Ivoire) with the WHO Infection Prevention and Control Assessment Framework (IPCAF)
Authors: Cissé Doumbia Mariamou , Essis Esme Marie Laure , Koné Atioumounan Blaise , Ngbesso Jean Paul Ngbesso , Mbengue Valérie Gbonon , Cissé Raïssa Adja Mayaka , Gagne Doh Eugénie , Dagnan N’cho Simplice , Kouadio Luc Philippe , Samba Mamadou 
Published: December 2022

Abstract
Introduction

As part of the implementation of its mission "to integrate hygiene activities into health-care", the general directorate of health conducted in 2018 with its technical structures, an evaluation of the implementation of Infection Prevention and Control (IPC) using the WHO IPCAF tool in 30 health-care facilities in the autonomous district of Abidjan.

Material and methods

This was a cross-sectional survey with a conceptualized component considering the issue of injection safety and sanitary waste management, which was conducted in the named health-care facilities from March 20 to 28, 2018. The scores of the essential components of the IPC made it possible to assess the IPC level of each health-care facility evaluated and the overall IPCAF score of all facilities.

Results

The overall median IPCAF score of the health-care facilities was 242.5/800 and corresponded to an inadequate level overall. No facility reached the "advanced" level of performance, 5 facilities (17%) reached the "intermediate" level, 10 (33%) fell into the "basic" level, and 15 (50%) were at the "inadequate" level. Baseline institutions had much higher scores than first contact institutions.

Conclusion

IPC component activities were inadequate and fragmented in the under-resourced health facilities at the time of the assessment. It would be appropriate to provide adequate resources and develop expertise in IPC through strong political will and leadership. This will contribute to the achievement of universal health insurance objectives with safe health services for patients.
UTX inhibition suppresses proliferation and promotes apoptosis in patient-derived glioblastoma stem cells by modulating periostin expression
Authors: Luan Yan , Liu Yingfei , Xue Jingwen , Wang Ke , Ma Kaige , Lu Haixia , Chen Xinlin , Liu Yong , Zhang Zhichao 
Published: December 2022

Abstract
Glioblastoma stem cells (GSCs) link tightly to glioblastoma (GBM) development, progression, therapeutic resistance and recurrence, suggesting GSCs as a novel target for drug discovery. UTX, a histone H3K27 demethylase, participates in regulating multiple cancer types. However, less is known about the function of UTX in GBM, let alone in GSCs. Our study aims to investigate the role and regulatory mechanism of UTX on GSCs. TCGA data showed that higher UTX expression was found in GBM and inversely correlated with survival. UTX inhibition hindered GBM cell growth and caused cell apoptosis. Subsequently, we cultured the primary GSCs, which were isolated from three patients. UTX inhibition suppressed cell proliferation and promoted apoptosis in GSCs. RNA-seq was performed to analyze the gene expression changes after silencing UTX in GSCs. The results indicated that UTX-mediated genes were strongly correlated with GBM progression and regulatory tumor microenvironment (TME). Transwell co-cultured experiment showed that silencing UTX in the transwell chamber GSCs could also inhibit the well plate cell proliferation. Protein-protein interaction analysis revealed that periostin (POSTN) played a role in the UTX-mediated transcriptional regulatory network. Replenishment of POSTN abolished the effect of UTX inhibition on GSCs proliferation and apoptosis, ablated the differential expression of intra- and extracellular levels of COL1A1 and VCAM1. Combining the above results together, our study demonstrated that UTX inhibition hindered POSTN expression by enhancing the H3K27me2/3 level, eventually resulting in inhibiting proliferation and promoting apoptosis of patient-derived GSCs. Our findings may provide a novel and effective strategy for the treatment of GBM.
Establishing a prognostic model of chromatin modulators and identifying potential drug candidates in renal clear cell patients Introduction
Authors: Liu Puyu , Luo Jihang , Tan Na , Li Chengfang , Xu Jieyu , Yang Xiaorong 
Published: December 2022

Abstract
Background: Renal carcinoma is a common malignant tumor of the urinary system. Advanced renal carcinoma has a low 5-year survival rate and a poor prognosis. More and more studies have confirmed that chromatin regulators(CRs) can regulate the occurrence and development of cancer. This article aims to investigate the functional and prognostic value of CRs in renal carcinoma patients.
Methods: mRNA expression and clinical information were obtained from the TCGA(The Cancer Genome Atlas)database. Univariate Cox regression analysis and LASSO regression analysis were used to select prognostic chromatin-regulated genes and use them to construct a risk model for predicting the prognosis of renal cancer. Differences in prognosis between high-risk and low-risk groups were compared using Kaplan-Meier analysis. In addition, we analyzed the relationship between chromatin regulators and tumor immune infiltration, and explored differences in drug sensitivity between different risk groups.
Results: We constructed a model consisting of 11 CRs to predict the prognosis of renal cancer patients, and not only successfully validated its feasibility, but we also found that the 11 CR-based model was an independent prognostic factor. Functional analysis showed that CRs were mainly enriched in cancer development-related signaling pathways. We also found through the TIMER database that CR-based models were also associated with immune cell infiltration and immune checkpoints.At the same time, the GDSC(The Genomics of Drug Sensitivity in Cancer database)database was used to analyze the commonly used drugs of KIRC(Renal clear cell carcinoma) patients. It was found that patients in the low-risk group were sensitive to drugs such as axitinib, pazopanib, sorafenib, and gemcitabine, while those in the high-risk group may be sensitive to sunitinib.
Conclusion: The chromatin regulator-related prognostic model we constructed can be used to assess the prognostic risk of patients with clear cell renal cell carcinoma. The results of this study can bring new ideas for targeted therapy of clear cell renal carcinoma, helping doctors to take corresponding measures in advance for patients with different risks.
LncTUG1 contributes to the progression of hepatocellular carcinoma via the miR-144-3p/RRAGD axis and mTOR/S6K pathway
Authors: Chen Weixi , Bai Zekun , Bai Wen , Wang Wei , Guo Jiapei , Guo Mengnan , Sai Yingying , Shi Jun , Wu Jinghua 
Published: December 2022

Abstract
Background: Hepatocellular carcinoma (HCC) is a symptomatic disease involed multi-stage program. Here, we elucidated the molecular mechanism of LncTUG1 in the regulation of HCC evolvement. And that may in all likelyhood supply a innovative latent target for HCC’s diagnoses and prognosis.
Methods: LncRNA TUG1, miR-144-3p, RRAGD and mTOR signaling pathway were screened as target genes in the database, and their expression levels at the cytological level were verified utilized qRT-PCR, Western Blot and immunohistochemistry. Then, we adopted CCK-8, Transwell and flow cytometry assays to estimate cell proliferation, invasion and apoptosis. By use of luciferase reporter assay, the relationships of LncRNA TUG1, miR-144-3p and RRAGD was confirmed. In addition, the LncRNA TUG1-miR-144-3p-RRAGD-mTOR signaling pathway in HCC cells was verified adopted rescue experiment and confirmed by xenotransplantation animal experiment
Results: LncTUG1 in HCC tissues from three databases were identified and further verified through qRT-PCR in HCC cells (Huh7, Hep3B). Knockdown the LncTUG1 could increase apoptosis and inhibite invasion and proliferation in HCC cells. Using inhibitors and activators of the mTOR/S6K pathway, LncTUG1 was confirmed to regulate HCC progression by the mTOR/S6K pathway. Luciferase reporter assay demonstrated that TUG1 negatively regulates miR-144-3p. Furthermore, miR-144-3p negativly regulates RRAGD by way of interacting with the 3’UTR of the RRAGD mRNA in HCC utilized luciferase reporter assay. In vivo, we also discovered that neoplasm weight and tumor volume reduced significantly in subcutaneous xenograft nude mouse models derived from sh-LncTUG1-expressing Huh7 cells. And the expressions of p-mTOR, p-S6K and RRAGD were decreased obviously while the miR144-3p increased in subcutaneous xenograft nude mouse models.
Conclusion: In a word, the research suggests that LncTUG1 targets miR-144-3p while miR-144-3p binds to RRAGD mRNA, which induces mTOR/S6K pathway activation and promotes the progression of HCC.
Lysinibacillus capsici 38328 Isolated from Agricultural Soils as a Promising Probiotic Candidate for Intestinal Health
Authors: Chen Huinan , Sun Xiaoyi , He Huilin , Ren Hongkun , Duan Haoyuan , Zhang Chuankun , Chang Qingru , Zhang Runxiang , Ge Junwei 
Published: December 2022

Abstract
There is an increasing interest in the use of spore-forming Bacillus spp. as probiotic ingredients on the market. However, probiotics Bacillus species are insufficient, and more safe Bacillus species were required. In the study, traditional fermented foods and soil samples were collected from all over the country in China, and 506 Bacillus were selected from 109 samples. Using the optimized procedure, we screened 9 strains, which successfully passed the acid, alkali, bile salt and trypsin resistance test. Drug sensitivity test results showed that three Bacillus out of the 9 isolates exhibited antibiotic sensitivity to more than 29 antibiotics. The 3 strains sensitive to antibiotics were identified by 16S ribosomal RNA, recA and gyrB gene analysis, two isolates (38327 and 38328) belong to the specie Lysinibacillus capsici and one isolate (37326) belong to Bacillus halotolerans. Moreover, the 3 strains were confirmed safe through animal experiments. Finally, L. capsici 38327 and 38328 showed the protection in Salmonella typhimurium infection mouse model, which manifested in slowing down weight loss, reducing bacterial load, improving antioxidant capacity. Altogether, our data demonstrate selected L. capsici strains can be used novel probiotics for intestinal health.
Effects of facial ugliness on the bad and ugly judgments of unethical behavior: an fMRI study
Authors: chengqiuping Qiuping , Zhou Ying , Zhang Yuman , Zhou Mi , Mo Lei 
Published: December 2022

Abstract
Objective: Unethical behavior has a powerful effect on an individual's overall judgment and is easily influenced by their looks. However, it remains unclear whether the unethical behavior of ugly-looking individuals is more likely to be judged as immoral and what the mechanisms behind it are. The present study investigates whether facial ugliness influences the bad and ugly judgments of unethical behavior from the perspective of neural mechanisms.
Method: The experimental material consisted of scenarios describing different levels of unethical behavior committed by ugly-looking or non-ugly-looking people. Subjects were asked to rate the bad and ugly levels of behaviors on a 4-point scale while being scanned for brain activity. We conducted a parametric general linear model (GLM) to estimate neural responses to facial ugliness in morally bad and ugly judgments, which varied with the level of immorality.
Results: The results showed that ugly-looking faces activated the anterior cingulate cortex and bilateral inferior occipital gyrus in both judgments, indicating a task-general pattern. Ugly-looking faces in morally ugly judgment showed stronger activity than in morally bad judgment in the ventromedial prefrontal cortex, left insula, left middle temporal gyrus, and cortical midline structures that characterize the emotional significance of events, suggesting a task-specific pattern.
Conclusions: The results of the current study demonstrate that facial ugliness is task-specific as well as task-general in evaluating unethical behaviors. The findings provide more experimental and neurological evidence for the understanding of the essence between morality and aesthetics.
Improving access to assessments of early motor development in local languages: Polish adaptation of the Early Motor Questionnaire
Authors: Laudańska Zuzanna , Szmytke Magdalena , Radkowska Alicja , Malinowska-Korczak Anna , Babis Karolina , Pérez David López , Tomalski Przemysław 
Published: December 2022

Abstract
A child’s motor development progresses very dynamically. It is crucial to develop freely available parent-report measures of motor development that can be easily used globally to measure motor skills and identify children in need of interventions. Here, we present the adaptation and validation of the Early Motor Questionnaire, which consists of gross motor (GM), fine motor (FM), and perception-action integration (PA) subscales, to the Polish language (EMQ-PL). In Study 1 (online, N=640), we observed its excellent psychometric properties and significant differences in GM and Total age-independent scores between children that were and were not referred for physiotherapy. In Study 2 (in-person assessment, longitudinal, N=100), we show high correlations of GM and total scores with Alberta Infant Motor Scale. Overall, we show that the EMQ can be easily adapted to local languages and has a potential for use as a screening tool.
Construction of Feature Analysis Model for Demeanor Evidence Investigation Based on Data Mining Algorithm
Authors: Zhang Mengxing , Qi Lin , Guo Yulong 
Published: December 2022

Abstract

Demeanor evidence refers to the authoritative opinions of experts using analytical instruments to collect and interpret the formation of external physiological symptoms of the entire human body.The use of man-made reasoning in the field of legal settling has become increasingly broad.Specialists are giving increasingly more consideration to the securing of modular proof. This paper proposes the development of a component examination model for modular proof examination applications. The technique for this paper is to apply the guileless Bayes strategy, propose a superior information mining calculation, and lay out a model for proof observation and examination. The function of these methods is to systematically explore the basic theoretical issues of demeanor evidence based on the status quo of judicial application of demeanor evidence. Through the prediction of individual demeanor based on data mining algorithm, the evidence analysis model is designed, the neurobiological experiment is carried out, and the demeanor evidence animal stress model is constructed to verify the scientific basis of multidemeanor evidence observation. The experimental results showed that after repeated stimulation of SD rats, the maximum changes in the expression of HSP70 gene and SAA gene were 10.77 and 14.1 respectively, reflecting the high reliability of demeanor evidence biological experiments.
Effect of Grandivittin from Ferulago trifida Boiss. on the proliferation and apoptosis of human lung cancer A549 cells
Authors: Anbaji Fatemeh Zomorodi , Zargar Seyed Jalal , Tavakoli Saeed 
Published: December 2022

Abstract
Lung cancer is one of the deadliest cancers in the world. Introducing new promising agents can help the chemotherapeutic management of cancer. In the knowledge of oncology, plants are of special interest as a rich source of new antineoplastic and chemotherapeutic agents. Grandivittin (GRA) is one of the main constituents of Fenzl (Ferulago trifida Boiss.) with established medicinal, phytochemical, and pharmacological properties. This study aimed to evaluate the antineoplastic potential of GAR and its underlying mechanisms in human lung cancer A549 cells. The viability of the A549 cells after being treated with different concentrations of GRA for three following days was measured using the MTT method. The early and late apoptosis were assessed by fluorescence-activated cell sorter analysis through annexin V/PI staining. The expression of apoptotic agents' genes (caspase3, caspase 9, Bcl2, Bax, and P53 ) was evaluated by the RT-PCR method. The GRA increased apoptotic cells and decreased cell viability in a dose- and time-dependent manner, in which only 50% of cells survived at a dose of 0.7 mM. The expression of Bax, P53, Caspase3, and caspase 9 genes in the A549 cells was significantly up-regulated after GRA treatment compared to control cells (P < 0.05). On the other hand, the Bcl2 was significantly down-regulated after GRA treatment (P < 0.05). The results indicated that the GRA can activate cell death in A549 lung carcinoma cells by ‎inducing both DNA toxicity p53 and cascade-dependent pathways. Therefore, the GRA may be a potential new therapeutic agent for the treatment of lung cancer.
The effects of omega-3 fatty acid used maternal rations during pregnancy on the fattening performances of male kids
Authors: Erez ibrahim , Serbester Uğur 
Published: December 2022

Abstract
In this study, fish oil was used as an omega-3 fatty acids source in pregnant goat’s rations in the different periods of pregnancy on mature male offsprings performance. Forty-seven male goat kids (84.6 ± 2.44 days or days old; 14.5 ± 3.09 kg body weight) were housed in individual pens and to evaluate live weight, feed consumption, serum biochemical parameters, carcass performance and meat quality characteristics for 56 days. Supplementation of fish oil to Anaç goats dam ration during the gestation periods decreased the fattening live weight and increased feed consumption (p=0.09), while body weight and feed conversion rates were not affected by experimental treatments (p=0.62, p=0.90). Fish oil increased serum AST, glucose, total protein, and globulin concentrations (p=0.02, p=0.01, p=0.03, p=0.02), while decreased heptadecanoic acid, oleic acid, linoleic acid, docosahexaenoic acid, and total mono- and polyunsaturated fatty acid concentrations in M. longissimus dorsi muscle (p=0.09, p=0.03, p=0.10, p=0.02, p=0.02, p=0.09). As a result, it has been thought that the use of liquid fish oil as an omega-3 source in goat’s ration during gestation may negatively affect the fetal development of male kids, but it has the ability to eliminate this negativity by improving post-weaning performance.