bluuebunny/crossref_metadata_2025_split huggingface dataset:
CDK actively contributes to establishment of the stationary phase state in fission yeast
Authors: Hiraoka Motoaki , Kiyota Yuki , Kawai Shinnosuke , Notsu Yusuke , Yamada Kohei , Kurashima Katsuyuki , Chang Jing-Wen , Shimazaki Shunsuke , Yamamoto Ayumu 
Published: May 2023

ABSTRACT
Upon exhaustion of essential environmental nutrients, unicellular organisms cease cell division and enter stationary phase, a metabolically repressed state essential for cell survival in stressful environments. In the fission yeast Schizosaccharomyces pombe, cell size is reduced by cell division before entry into stationary phase; thus cyclin-dependent kinase (CDK) must actively contribute to stationary phase establishment. However, the contribution of CDK to stationary phase remains largely uncharacterized. Here, we examine the role of the sole S. pombe CDK, Cdc2, in the establishment of stationary phase. We show that in stationary phase, nuclear and chromosomal volumes and the nucleus-to-cell volume ratio are reduced, and sister chromatid separation and chromosome fluctuation are repressed. Furthermore, Cdc2 accumulates in the nucleolus. Most of these changes are induced by glucose depletion. Reduction in Cdc2 activity before and upon stationary phase entry alleviates the changes and shortens the survival time of stationary phase cells, whereas Cdc2 inhibition represses nucleolar Cdc2 accumulation and glucose depletion-induced nuclear volume reduction. These results demonstrate that CDK actively regulates stationary phase, both before and upon stationary phase entry.
Nowe gatunki grzybów makroskopijnych w biocie Wigierskiego Parku Narodowego
Authors: Halama Marek , Pencakowski Bartosz , Fałtynowicz Wiesław , Leski Tomasz , Patejuk Katarzyna , Wilgan Robin , Kowalewska Agnieszka , Fałtynowicz Hanna , Piegdoń Amelia , Staniaszek-Kik Monika , Górski Piotr , Romański Maciej , Picińska-Fałtynowicz Joanna , Kapruś Ihor , Krzysztofiak Lech 
Published: May 2023

In the year 2021 mycological investigations were carried out in the Wigierski National Park (NE Poland). Fifteen 0.04-ha plots, including 5 with low proportion of hazel (< 30%) and 10 with high proportion of hazel (≥ 75%) in the understory, were established in phytocoenoses of a mesophilous oak-linden hornbeam forest (Tilio cordatae-Carpinetum betuli). Macrofungi were surveyed on these plots during the summer and autumn season based on the presence/absence of sporocarps. As a result, 320 taxa of fungi have been recorded, which accounts for ca 28% of macrofungi biota of the park. During this survey 28 species new to this area have been found. The paper provides information about their localities and habitats.
The impact of pharmacist behavioral intervention on antibiotics prescribing in pediatric wards
Authors: Abbas Jasim Kadhim , Al-Metwali Basma Zuheir 
Published: May 2023

Background: Antibiotics may be prescribed irrationally, which can lead to significant consequences including the emergence of antimicrobial resistance. Pharmacists can play a key role in enhancing the rational antibiotic prescribing. This study aimed to evaluate the current clinical practice of prescribing antimicrobial agents in pediatric wards and to assess the clinical and economic impact of pharmacist interventions on antibiotic prescribing. Methodology: This study was conducted at the pediatric wards of a general hospital, Babel, Iraq. The study population included all pediatric inpatients aged ≤ 12 years and receiving antibiotic. The study consisted of three phases. The first was a pre-intervention phase where data about antibiotic prescription was collected retrospectively. The second phase involved pharmacist intervention where the researcher had provided an oral presentation to the healthcare workers. The third phase involved prospective data collection which was compared with the pre-intervention data. Independent T-test was used to compare the differences in the parameters between the pre-and post-intervention phases. Results: The study included 250 children in each of the pre- and post-intervention phases who received antibiotic treatment. The medical records were reviewed retrospectively. Four antibiotics (amoxicillin, cefotaxime, vancomycin and meropenem) were significantly less frequently used in the post-intervention phase. Single antibiotic treatment was significantly higher in the post-intervention phase (p-value<0.05). Length and days of therapy, and the total cost of the antibiotics used were significantly lower in the post-intervention phase. Culture and sensitivity tests were performed only in 28% of patients in the post-intervention phase where the isolated bacteria were found to be highly resistant to penicillin and cephalosporin. Conclusions: This study demonstrated that pharmacist intervention in general pediatric wards has resulted in favorable clinical and economic outcomes. This emphasizes the essential role pharmacists can play in rational antibiotic use, not only in the pediatric population but also in the adults.
Uptake of same-day initiation of HIV treatment in Malawi, South Africa, and Zambia as reported in routinely collected data: the SPRINT retrospective cohort study
Authors: Huber Amy , Hirasen Kamban , Brennan Alana T. , Phiri Bevis , Tcherini Timothy , Mulenga Lloyd , Haimbe Prudence , Shakwelele Hilda , Nyirenda Rose , Wilson Matola Bilaal , Gunda Andrews , Rosen Sydney 
Published: May 2023

Background: Since 2017 global guidelines have recommended “same-day initiation” (SDI) of antiretroviral treatment (ART) for patients considered ready for treatment on the day of HIV diagnosis. Many countries have incorporated a SDI option into national guidelines, but SDI uptake is not well documented. We estimated average time to ART initiation at 12 public healthcare facilities in Malawi, five in South Africa, and 12 in Zambia. Methods: We identified patients eligible to start ART between January 2018 and June 2019 from facility testing registers and reviewed their medical records from HIV diagnosis to the earlier date of treatment initiation or 6 months. We estimated the proportion of patients initiating ART on the same day or within 7, 14, 30, or 180 days of baseline. Results: We enrolled 825 patients in Malawi, 534 in South Africa, and 1,984 in Zambia. Overall, 88% of patients in Malawi, 57% in South Africa, and 91% in Zambia received SDI. In Malawi, most who did not receive SDI had not initiated ART ≤6 months. In South Africa, an additional 13% initiated ≤1 week, but 21% had no record of initiation ≤6 months. Among those who did initiate within 6 months in Zambia, most started ≤1 week. There were no major differences by sex. WHO Stage III/IV and tuberculosis symptoms were associated with delays in ART initiation; clinic size and having a CD4 count done were associated with an increased likelihood of SDI. Conclusions: As of 2020, SDI of ART was widespread, if not nearly universal, in Malawi and Zambia but considerably less common in South Africa. Limitations of the study include pre-COVID-19 data that do not reflect pandemic adaptations and potentially missing data for Zambia. South Africa may be able to increase overall ART coverage by reducing numbers of patients who do not initiate ≤6 months.
The ERC PuppetPlays project: contribution for a non-linear history of the European theatre
Authors: Plassard Didier 
Published: May 2023

This article is a presentation of the ERC Advanced Grant project PuppetPlays - Reappraising Western European Repertoires for Puppet and Marionette Theatre (GA 835193). After a short overview of the project itself, it begins with a definition of puppetry, based on the phenomenon of double vision. Then it explains the choice of the corpus limitations, describes the variety of the available resources, and underlines the great discrepancy in the amount of material available in the different countries. The article continues with a brief overview of the role played by puppetry in the wider frame of performing arts: how much can we consider that puppeteers developed specific repertoires? What kind of differences can be observed between puppet or marionette theatre and actors’ theatre? The answers to these questions differ in a considerable way according to the cultural and sociological contexts: sometimes puppet and marionette theatre were the only forms of performance allowed, and they acted as substitutes for actors theatre; but sometimes also - and this is increasingly the case since the end of the 19th century - these instruments were chosen for their specific expressive qualities. In a last movement, I emphasize that collecting and analyzing puppet and marionette repertoires brings us to reconsider the general historiography of theatre: firstly, because we bring into the light theatrical genres that have been neglected by the historians; and secondly, because the plays written by the puppeteers, when we look closely at them, reveal a stratification of different layers that can be considered as a kind of heterochrony ; an alternative construction to social time. The forgotten patrimony of puppet and marionette dramaturgy conceals therefore many possibilities for research in humanities and social sciences.
Characterisation of human exposure to nocturnal biting by malaria and arbovirus vectors in a rural community in Chókwè district, southern Mozambique
Authors: Kampango Ayubo , Pinto João , Abílio Ana Paula , Machoe Elias , Matusse Júlio , McCall Philip J. 
Published: May 2023

Background: Understanding the magnitude of human exposure to mosquito biting is fundamental to reduce pathogen transmission. Here we report on a study quantifying the levels of mosquitoes attacking humans throughout the night in a rural area of Southern Mozambique. Methods: Surveys were carried out in Massavasse village, southern Mozambique. The abundance and composition of host-seeking mosquito communities at night were assessed by human-landing catches (HLC) at one-hour intervals. Periods when people were located predominantly outdoors or indoors were used to estimate the amount of residents’ exposure to mosquito bites in either location, to explore the potential impact a bed net could have had in reducing biting by each vector species. Results: A total of 69,758 host-seeking female mosquitoes comprising 23 species in four genera were collected. The exposure to biting by virtually all vector species was consistently high outdoors, typically at early evening and morning, with exception of An. gambiae s.l which was likely of biting a person with nearly same intensity indoors and outdoors throughout the night. Bed nets use could have reduced biting by An. gambiae s.l (dominated by An. arabiensis), Ma. africana, Ma. uniformis, Cx. pipiens, Cx. antennatus, and Cx. poicilipes by 53%, 47%, 46%, 38%, 31%, and 28% respectively, compared to non-users. Conversely, a bed net user would have had little protection against An. pharoensis, An. ziemanni, An. tenebrosus, and Cx. tritaeniorhynchus biting exposures. Conclusions: This study showed that Massavasse residents were exposed to high levels of outdoor biting by malaria and arbovirus vectors that abound in the village. The findings help to identify entomological drivers of persistent malaria transmission in Mozambique and identify a wide range of arbovirus vectors nocturnally active in rural areas, many with outbreak potential. The study highlights the need for a surveillance system for monitoring arboviral diseases vectors in Mozambique.
A rapid review of community engagement and informed consent processes for adaptive platform trials and alternative design trials for public health emergencies
Authors: Davies Alun , Ormel Ilja , Bernier Alexe , Harriss Eli , Mumba Noni , Gobat Nina , Schwartz Lisa , Cheah Phaik Yeong 
Published: May 2023

Background: Public Health Emergencies (PHE) demand expeditious research responses to evaluate new or repurposed therapies and prevention strategies. Alternative Design Trials (ADTs) and Adaptive Platform Trials (APTs) have enabled efficient large-scale testing of biomedical interventions during recent PHEs. Design features of these trials may have implications for engagement and/or informed consent processes. We aimed to rapidly review evidence on engagement and informed consent for ADTs and APTs during PHE to consider what (if any) recommendations can inform practice. Method: In 2022, we searched 8 prominent databases for relevant peer reviewed publications and guidelines for ADTs/APTs in PHE contexts. Articles were selected based on pre-identified inclusion and exclusion criteria. We reviewed protocols and informed consent documents for a sample of large platform trials and consulted with key informants from ADTs/APT trial teams. Data were extracted and summarised using narrative synthesis. Results: Of the 49 articles included, 10 were guidance documents, 14 discussed engagement, 10 discussed informed consent, and 15 discussed both. Included articles addressed ADTs delivered during the West African Ebola epidemic and APTs delivered during COVID-19. PHE clinical research guidance documents highlight the value of ADTs/APTs and the importance of community engagement, but do not provide practice-specific guidance for engagement or informed consent. Engagement and consent practice for ADTs conducted during the West African Ebola epidemic have been well-documented. For COVID-19, engagement and consent practice was described for APTs primarily delivered in high income countries with well-developed health service structures. A key consideration is strong communication of the complexity of trial design in clear, accessible ways. Conclusion: We highlight key considerations for best practice in community engagement and informed consent relevant to ADTs and APTs for PHEs which may helpfully be included in future guidance. Protocol: The review protocol is published online at Prospero on 15/06/2022: registration number CRD42022334170.
Amino acid score of some products for younger school children nutrition
Authors: Vatan M. , Babienko V. 
Published: May 2023

Introduction. Wholesome nutrition is one of the most important factors in shaping a person's somatic and mental health. Eating habits are formed in childhood and consolidated in adulthood. They can also have an impact on the development of future chronic diseases, such as metabolic syndrome, type 2 diabetes, cardiovascular diseases. The purpose of the work is to evaluate the amino acid score of the main food products of children of primary school age.
Material and methods. The research was carried out during 2016 - 2021. Food menus for 1000 of children aged 6-9 years old living in the South of Ukraine (cities Odessa and Mykolaiv, suburban villages and villages far from the regional centres). The study was carried out during the spring-summer (May-June) and autumn-winter (November-December). Field studies of the amino acid content in 24 products, which were included in the first two deciles in terms of frequency of consumption, were also conducted. The results. The distribution of food products was marked by a certain constancy and depended little on the season of the year. Most foods are a major source of carbohydrates. Only 8 out of 24 products were a source of protein and essential amino acids. The overall consumption of protein-rich foods by younger schoolchildren was low. Conclusion. Children 6-10 years old should consume protein in the amount of 0.95 g/kg/day. Our research shows that even such an understated standard of need may not be fulfilled under the current state of affairs. The main sources of protein for children of primary school age in Southern Ukraine are milk, cheese, chicken, river fish and legumes. Dairy products, eggs and chicken have the highest value in terms of amino acid content, and pork has the lowest value. Legumes have a high amino acid content, but they are inferior to products of animal origin.
Towards real-time active imaging of greenhouse gases using tunable mid-infrared all-fiber lasers
Authors: Michaud Louis-Charles , Boilard Tommy , Magnan-Saucier Sébastien , Paradis Pascal , Talbot Lauris , Thiboult Antoine , Nadeau Daniel F. , Vallée Réal , Bernier Martin 
Published: May 2023

We report a tunable all-fiber laser emitting a maximum output power of 2.55 W around 3240 nm. The fiber laser cavity based on a fluoride fiber doped with dysprosium ions yields an efficiency of 42% according to the in-band launched pump power at 2825 nm. Due to a custom piezoelectric fiber Bragg grating (FBG) package, mechanical strains applied to the narrowband FBG used as the input cavity coupler allowed for fast tuning of the emission wavelength over a spectral range of 1.5 nm. This laser was deployed in the field in northern Québec (Canada) to assess its performances for remote sensing of methane in the presence of a significant amount of water vapor, i.e., over a hydroelectric reservoir. The preliminary results acquired during this field campaign confirm the great potential of the proposed approach for the development of a real-time active imaging system of greenhouse gases.
Chikungunya virus infection in Aedes aegypti is modulated by L-cysteine, taurine, hypotaurine and glutathione metabolism
Authors: Kumar Ankit , Shrinet Jatin , Sunil Sujatha 
Published: May 2023

Background
Blood meal and infections cause redox imbalance and oxidative damage in mosquitoes which triggers the mosquito’s system to produce antioxidants in response to increased oxidative stress. Important pathways activated owing to redox imbalance include taurine, hypotaurine and glutathione metabolism. The present study was undertaken to evaluate the role of these pathways during chikungunya virus (CHIKV) infection in Aedes aegypti mosquitoes.

Methodology
Using a dietary L-cysteine supplement system, we upregulated these pathways and evaluated oxidative damage and oxidative stress response upon CHIKV infection using protein carbonylation and GST assays. Further, using a dsRNA based approach, we silenced some of the genes involved in synthesis and transport of taurine and hypotaurine and then evaluated the impact of these genes on CHIKV infection and redox biology in the mosquitoes.

Conclusions
We report that CHIKV infection exerts oxidative stress in the A. aegypti, leading to oxidative damage and as a response, an elevated GST activity was observed. It was also observed that dietary L-cysteine treatment restricted CHIKV infection in A. aegypti mosquitoes. This L-cysteine mediated CHIKV inhibition was coincided by enhanced GST activity that further resulted in reduced oxidative damage during the infection. We also report that silencing of genes involved in synthesis of taurine and hypotaurine modulates CHIKV infection and redox biology of Aedes mosquitoes during the infection.
The burden of chronic diseases, disease-stratified exploration and gender-differentiated healthcare utilisation among patients in Bangladesh
Authors: Mahumud Rashidul Alam , Gow Jeff , Mosharaf Md Parvez , Kundu Satyajit , Rahman Md. Ashfikur , Dukhi Natisha , Shahajalal Md , Mistry Sabuj Kanti , Alam Khorshed 
Published: May 2023

Background
Chronic diseases are considered one of the major causes of illness, disability, and death worldwide. Chronic illness leads to a huge health and economic burden, especially in low- and middle-income countries. This study examined disease-stratified healthcare utilisation (HCU) among Bangladesh patients with chronic diseases from a gender perspective.

Methods
Data from the nationally representative Household Income and Expenditure Survey 2016–2017 consisting of 12,005 patients with diagnosed chronic diseases was used. Gender differentiated chronic disease stratified-analytical exploration was performed to identify the potential factors to higher or lower utilisation of healthcare services. Logistic regression with step-by-step adjustment for independent confounding factors was the method used.

Results
The five most prevalent chronic diseases among patients were gastric/ulcer (Male/Female, M/F: 16.77%/16.40%), arthritis/rheumatism (M/F: 13.70%/ 13.86%), respiratory diseases/asthma/bronchitis (M/F: 12.09% / 12.55%), chronic heart disease (M/F: 8.30% / 7.41%), and blood pressure (M/F: 8.20% / 8.87%). Eighty-six percent of patients with chronic diseases utilised health care services during the previous 30 days. Although most patients received outpatient healthcare services, a substantial difference in HCU among employed male (53%) and female (8%) patients were observed. Chronic heart disease patients were more likely to utilise health care than other disease types, which held true for both genders while the magnitude of HCU was significantly higher in males (OR = 2.22; 95% CI:1.51–3.26) than their female counterparts (OR = 1.44; 1.02–2.04). A similar association was observed among patients with diabetes and respiratory diseases.

Conclusion
A burden of chronic diseases was observed in Bangladesh. Patients with chronic heart disease utilised more healthcare services than patients experiencing other chronic diseases. The distribution of HCU varied by patient’s gender as well as their employment status. Risk-pooling mechanisms and access to free or low-cost healthcare services among the most disadvantaged people in society might enhance reaching universal health coverage.
Association between smoking behavior and serum uric acid among the adults: Findings from a national cross-sectional study
Authors: Jang Yun Seo , Nerobkova Nataliya , Yun Il , Kim Hyunkyu , Park Eun-Cheol 
Published: May 2023

Background
Gout incidence is increasing worldwide; appropriate management of serum uric acid levels and a healthy lifestyle may help its prevention. The popularity of electronic cigarettes and the resultant emergence of dual smokers is increasing. Despite many studies on the effects of various health behaviors on serum uric acid levels, the association between smoking and serum uric acid levels remains controversial. This study aimed to investigate the association between smoking and serum uric acid levels.

Methods
In this study, total sample of 27,013 participants (11,924 men and 15,089 women) were analyzed. This study used data from the Korea National Health and Nutrition Examination Survey (2016–2020) and grouped adults into dual smokers, single smokers, ex-smokers, and non-smokers. Multiple logistic regression analyses were performed to investigate the association between smoking behavior and serum uric acid levels.

Results
Compared to male non-smokers, male dual smokers had significantly higher serum uric acid level (odds ratio [OR], 1.43; 95% confidence interval [CI], 1.08–1.88). In female, serum uric acid level was higher among single smokers than non-smokers (OR, 1.68; 95% CI, 1.25–2.25). Higher serum uric acid levels were more likely to be present in male dual smokers with a > 20 pack-year smoking habit (OR, 1.84; 95% CI, 1.06–3.18).

Conclusion
Dual smoking may contribute to high serum uric acid levels in adults. Thus, serum uric acid levels should be properly managed through smoking cessation.
Bombay Cinema’s Islamicate Histories, Ira Bhaskar and Richard Allen (Eds) (2022)
Authors: Wadhwa Soni 
Published: May 2023

Review of: Bombay Cinema’s Islamicate Histories, Ira Bhaskar and Richard Allen (Eds) (2022)
Hyderabad: Orient BlackSwan, 424 pp.,
ISBN 978-9-35442-257-7, p/bk, ₹2295