bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Enterobacterales growing on selective CPE screening plates with a focus on non-carbapenemase-producing strains
Authors: Efrati Epchtien Reut , Temkin Elizabeth , Lurie-Weinberger Mor N. , Kastel Ophir , Keren-Paz Alona , Schwartz David , Carmeli Yehuda 
Published: January 2025

ABSTRACT

Carbapenem-resistant Enterobacterales (CRE) are divided into two distinct groups: carbapenemase-producing (CPE) and non-carbapenemase-producing (non-CPE). The population of non-CPE growing on CPE selective plates during routine screening is usually not reported and is not well defined. This study aimed to characterize non-CPE isolates growing on those plates. Isolates were collected from two medical institutions in Israel between May and November 2022. Species identification and antibiotic susceptibility testing were performed using VITEK systems. Further analyses focused on
Klebsiella pneumoniae
and
Escherichia coli
which were the most common isolates. Meropenem MIC was determined by E-test. Fourier-transform infrared spectroscopy (FTIR) was used to analyze phenotypic similarity, and whole genome sequencing was conducted on a subset of non-CPE isolates. Of 260 isolates growing on selective CPE screening plates, 60.4% were non-CPE. The most common non-CP isolates were
K. pneumoniae
(65.6%) followed by
E. coli
(20.4%). Of the non-CP
K. pneumoniae
and
E. coli
isolates, 78.2% were susceptible to meropenem. All non-CP
K. pneumoniae
and
E. coli
isolates were multidrug-resistant (MDR) and belonged to multiple FTIR clusters. The 21 sequenced non-CPE isolates carried multiple mechanisms of resistance. Isolates had multiple beta-lactamases and all had penicillin-binding protein modifications and porin mutations; in meropenem-resistant
K. pneumoniae
isolates, both Ompk35 and Ompk36 were mutated. The majority of isolates growing on selective CPE screening plates are non-CPE but are MDR. Laboratory reporting of these MDR bacteria might be useful for guiding treatment and prophylaxis when indicated, as well as for infection control.

IMPORTANCE
Selective screening plates for carbapenemase-producing Enterobacterales (CPE) are used to detect CPE carriers for infection control purposes. We characterized non-CPE isolates that grew on selective CPE screening plates, which are intended to filter them out. We found that 60% of isolates that grew on these plates were not CPE. They included both meropenem-susceptible and meropenem-resistant isolates and were multidrug-resistant with multiple resistance mechanisms. These test results, which are usually not reported by laboratories, may be clinically valuable.
Gamma attenuation and radiation shielding properties of lead silicate glasses containing antimony and alumina oxides
Authors: Alrowaili Z. A. , Alomayrah Norah , Saleh H. H. , Sriwunkum Chahkrit , Alalawi Amani , Al-Buriahi M. S. 
Published: January 2025

Abstract
Lead silicate glasses have been widely used in various applications, including radiation shielding, due to their high density and effective atomic number. However, the addition of certain oxides to the glass composition can significantly enhance their radiation shielding properties. In this study, we investigate the effect of incorporating antimony and alumina oxides on the photon attenuation and shielding properties of lead silicate glasses. It is found that the HVL values of the APSSS1 sample started from 0.00239 cm at photon energy of 0.015 MeV and increased with increasing energy to a maximum value of 4.223 cm at 6 MeV photon energy then decreased to 3.767 cm at energy of 15 MeV. Moreover, the shielding ability of the studied samples are compared with commercial glassy materials. The results of the study showed that the addition of antimony and alumina oxides significantly improved the radiation shielding properties of the lead silicate glasses.
JPEG Column: 101st JPEG Meeting
Authors: Pinheiro Antonio 
Published: January 2025

JPEG Trust reaches Committee Draft stage at the 101st JPEG meeting.
The 101st JPEG meeting was held online, from the 30th of October to the 3rd of November 2023. At this meeting, JPEG Trust became a Committee Draft. In addition, JPEG analyzed the responses to its Calls for Proposals for JPEG DNA.
Enhancing Sulfinpyrazone determination via Electrochemistry: Development of a S-Doped CoFe2O4 Modified Sensor
Authors: Kurundawade Sandeep , Patil Yuvarajgouda N. , Megalamani Manjunath B. , Nandibewoor Sharanappa 
Published: January 2025

Abstract
An electrochemical sensor for the electrochemical determination of Sulfinpyrazone (SFP) was developed based on ferrite, a Sulphur-doped Cobalt Ferrite (S- CoFe2O4), functionalized glassy carbon electrode (GCE). SFP displayed remarkably increased electrochemical activity on the S-CoFe2O4 modified GCE as the modifier enhances the surface area and electrocatalytic activity. Characterization of the electrode modifier was carried out through XRD and SEM-EDS analyses. Cyclic voltammetry (CV) and square wave voltammetry (SWV) were utilized to explore SFP analysis. The optimum immersion time for the study was found to be 20 seconds. An enhanced oxidation of SFP was noticed at a pH of 7.4 for the investigation between the potential range of 0 to 1.0 V. The process displayed irreversible and diffusion-controlled nature. Under ideal experimental conditions, the oxidation peak current of SFP showed a direct proportionality to its concentration within the range of 1.0 to 10 µM, with a detection limit of 2.45 nM. This sensor effectively determines the determination of SFP in pharmaceutical products, yielding satisfactory results in terms of recovery, stability, and sensitivity. This approach creates opportunity for exploration in the electrochemical investigation of SFP. These findings suggest that the constructed sensor could be useful in pharmacokinetic studies and quality control labs.
Comparative Single-Cell Transcriptomics of Human Neuroblastoma and Preclinical Models Reveals Conservation of an Adrenergic Cell State
Authors: Embaie Bethel Tesfai , Sarkar Hirak , Alchahin Adele Mirna. , Otte Jörg , Olsen Thale Kristin. , Tümmler Conny , Kameneva Polina , Artemov Artem V. , Akkuratova Natalia , Adameyko Igor , Stukenborg Jan-Bernd , Wickström Malin , Kogner Per , Johnsen John Inge , Mei Shenglin , Kharchenko Peter V. , Baryawno Ninib 
Published: January 2025

Abstract
Transgenic mice and organoid models, such as three-dimensional tumoroid cultures, have emerged as powerful tools for investigating cancer development and targeted therapies. Yet, the extent to which these preclinical models recapitulate the cellular identity of heterogeneous malignancies, like neuroblastoma (NB), remains to be validated. Here, we characterized the transcriptional landscape of TH-MYCN tumors by single-cell RNA sequencing (scRNA-seq) and developed ex vivo tumoroids. Integrated analysis with murine fetal adrenal samples confirmed that both TH-MYCN tumors and tumoroids closely mirror the cellular profiles of normal embryonic sympathoblasts and chromaffin cells. Comprehensive comparison between tumors from NB patients and TH-MYCN mice demonstrated similarities in adrenergic tumor cell composition. Ex vivo tumoroid cultures displayed histological resemblance and shared transcriptional profiles with the originating TH-MYCN tumors and human NB. Importantly, subpopulations within tumoroids exhibited gene expression associated with poor NB patient survival. Notably, recurrent observations of a low-proliferative chromaffin phenotype connected to the highly proliferative sympathetic phenotype suggested that pushing sympathoblasts into a chromaffin-like state may offer an interesting therapeutic strategy for NB. Together, this study not only deepens our understanding of a widely used transgenic mouse NB model but also introduces an ex vivo model that maintains critical adrenergic cell state identity, thereby enhancing its translational potential for NB research.
Evolutionary Pressures Shape Undifferentiated Pleomorphic Sarcoma Development and Radiotherapy Response
Authors: Blomain Erik , Soudi Shaghayegh , Wang Ziwei , Somani Anish , Subramanian Ajay , Nouth Serey C.L. , Oladipo Eniola , New Chistin , Kenney Deborah E. , Nemat-Gorgani Neda , Kindler Thomas , Avedian Raffi S. , Steffner Robert J. , Mohler David G. , Hiniker Susan M. , Chin Alexander L. , Kalbasi Anusha , Binkley Michael S. , Fried Marius , Gaida Matthias M. , van de Rijn Matt , Moding Everett J. 
Published: January 2025

Abstract
Radiotherapy is an integral component in the treatment of many types of cancer, with approximately half of cancer patients receiving radiotherapy. Systemic therapy applies pressure that can select for resistant tumor subpopulations, underscoring the importance of understanding how radiation impacts tumor evolution to improve treatment outcomes. We integrated temporal genomic profiling of 120 spatially distinct tumor regions from 20 patients with undifferentiated pleomorphic sarcomas (UPS), longitudinal circulating tumor DNA (ctDNA) analysis, and evolutionary biology computational pipelines to study UPS evolution during tumorigenesis and in response to radiotherapy. Most unirradiated UPS displayed initial linear evolution followed by subsequent branching evolution with distinct mutational processes during early and late development. Metrics of genetic divergence between regions provided evidence of strong selection pressures during UPS development that further increased during radiotherapy. Subclone abundance changed following radiotherapy with subclone contraction tied to alterations in calcium signaling, and inhibiting calcium transporters radiosensitized sarcoma cells. Finally, ctDNA analysis accurately measured subclone abundance and enabled non-invasive monitoring of subclonal changes. These results demonstrate that radiation exerts selective pressures on UPS and suggest that targeting radioresistant subclonal populations could improve outcomes after radiotherapy.
PIN1 Prolyl Isomerase Promotes Initiation and Progression of Bladder Cancer through the SREBP2-Mediated Cholesterol Biosynthesis Pathway
Authors: Wang Xue , Lee Derrick , Xu Haibo , Sui Yuan , Meisenhelder Jill , Hunter Tony 
Published: January 2025

Abstract
Identities of functional pSer-Thr.Pro protein substrates of the PIN1 prolyl isomerase and its effects on downstream signaling in bladder carcinogenesis remain largely unknown. Phenotypically, we found that PIN1 positively regulated bladder cancer cell proliferation, cell motility, and urothelium clearance capacity in vitro and controlled tumor growth and potential metastasis in vivo. Mechanistically, we observed a negative enrichment of SREBP2-driven cholesterol metabolism pathways and a decrease in free/total cholesterol levels in PIN1-knockout bladder cancer cells. Moreover, we showed that PIN1 interacted with SREBP2 following its phosphorylation by the JNK MAP kinase at Ser455, which lies near the site-2 cleavage site that generates the active, nuclear form of SREBP2. Therapeutically, a combination of the covalent PIN1 inhibitor sulfopin and the HMGCoA reductase inhibitor simvastatin synergistically suppressed cell proliferation in vitro and tumor growth in vivo. Together, these findings emphasize that PIN1 can act as a driver and potential therapeutic target in bladder cancer.

Significance:
This study provides deeper insights into the regulatory role of the phospho–dependent prolyl isomerase PIN1 in bladder cancer. The identification of the link between PIN1 and SREBP2-mediated transcription and cholesterol biosynthesis offers the potential for developing novel therapeutic strategies for bladder cancer.
Real-World Incidence of Anaplastic Lymphoma Kinase Alterations in Hispanics with Non–Small Cell Lung Cancer at a Large Academic Institution in Los Angeles
Authors: Poei Darin , Ali Sana , Thomas Jacob S. , Nieva Jorge J. , Hsu Robert C. 
Published: January 2025

Abstract
Race and ethnicity affect the distribution of molecular alterations seen in patients with non–small cell lung cancer (NSCLC). Although anaplastic lymphoma kinase (ALK) alterations are known to occur in 4% to 5% of the population, data specific to the Hispanic population remain limited. This study describes the real-world incidence of ALK alterations in Hispanic patients with NSCLC treated at a large academic institution in Los Angeles, California, United States, to further elucidate the underlying factors that shape differences in mutational profiles. A total of 607 patients with NSCLC treated at Los Angeles General Medical Center (n = 172) and the University of Southern California Norris Comprehensive Cancer Center (n = 435) who received comprehensive genomic profiling were evaluated from January 2015 to June 2023. The Fisher exact test and multivariate logistic regression were incorporated for statistical analysis. Hispanic patients exhibited a higher incidence of ALK alterations (12.76%, n = 18/141) than non-Hispanic patients (5.36%, n = 23/466; P = 0.0046). Multivariate logistic regression showed that Hispanic ethnicity (HR, 2.393; 95% confidence interval, 1.115–5.092) and age at diagnosis (HR, 0.9325; 95% confidence interval, 0.9081–0.9558) were significant variables in ALK alteration incidence, and 55.81% (n = 24) of ALK alterations were initially found via comprehensive genomic profiling. This study highlights a significantly higher incidence of ALK alterations among Hispanic patients with NSCLC, emphasizing the impact of race and ethnicity on molecular alterations. Future research is required to evaluate this trend in a larger, more diverse cohort and investigate the roles of environmental factors and tumor microenvironments in influencing ALK alteration prevalence.

Significance:
This study identified a higher incidence of ALK alterations in Hispanic patients with NSCLC (12.76%) compared with that in non-Hispanic patients (5.36%) treated at a large academic center in Los Angeles, highlighting the impact of race on molecular alteration profiles and emphasizing the need to increase access to molecular analyses for this population. The variability in mutational alterations may be influenced by biological and environmental factors.
Du sens II : les passions entre sémiotique narrative et sémiotique discursive
Authors: Fontanille Jacques 
Published: January 2025

La conception des passions, dans le champ de la sémiotique structurale, est depuis le début, dans Sémantique structurale (Greimas), jusqu’à plus récemment dans Sémiotique des passions (Greimas et Fontanille) marquée par un double ancrage : un ancrage dans le système immanent des énoncés narratifs et des modalités des sujets d’état, et un autre ancrage dans la manifestation discursive et les articulations entre actions, interactions et passions. Cette étude, centrée sur Du sens II, navigue néanmoins entre l’amont et l’aval de cet ouvrage, et y compris chez les auteurs (Coquet, Landowski) qui ont pris quelque distance avec la sémiotique narrative de Greimas. On peut examiner en détail les conséquences de cette tension entre deux conceptions différentes de la passion, et ce détail, on le verra, explique bien des difficultés déjà identifiées. Mais on doit aussi tenter de résoudre cette tension, et plus explicitement articuler le système général des descripteurs disponibles, et les compositions catégorielles particulières (les régimes sémiotiques) dont la manifestation discursive se constitue.
A fundamentação ontológica da estrutura ética do juízo estético no primeiro-romantismo alemão: para construção histórico-sistemática do Juízo Infinito
Authors: Ramos Gabriel  Loureiro Pereira da Mota 
Published: January 2025

Reading the fragments of Schlegel and Novalis in Blüthenstaub (2016), the article seeks to understand how the romantic thinkers ontologize Kant's argument regarding aesthetic judgment. To do this, we read the Analitik des Schönen, from the Kritik der Urteilskraft (1974b), to understand how the ethical structure of aesthetic judgment, suggested in Kant only by the as-if clause, receives an ontological interpretation in Novalis and Schlegel. We thus develop the figure of unending judgment, a concept with which we seek to circumscribe the triple movement by which Schlegel and Novalis ontologically ground the ethical structure of aesthetic judgment.
Validation Status of Electronic Sphygmomanometers in China: A National Survey
Authors: Zhang Xianghui , Xu Zhimin , Yao Yingchun , Zheng Huilei , Wu Jian , Hu Jingwen , Wu Shenggen , Lyu Quanjun , Liu Yong , Wu Min , Chen Liangxia , Xu Lina , Zhou Haiyan , Duan Peifen , Dai Jianghong , Ding Hong , Wang Shuang , Zhao Yi , Hu Jihong , Zhu Liyue , Hai Rong , Zhong Baoliang , Zhu Huilian , Li Kui , Lu Yao , Tang Shaowen , Li Xingming , Zhang Yan , Chen Zongtao , Wang Tongyu , Li Mei , Liu Fuxu , Liu Chengluan , Yang Xiaoling , Dong Lihua , Ma Jixiang , Gao Pei , Xie Wuxiang , Wu Yangfeng 
Published: January 2025

BACKGROUND:
Although the information on the validation status of electronic sphygmomanometer (ES) devices in use in health care institutions and households is much more clinically relevant than that of ES models available on the market, it remains insufficient.

METHODS:
A national survey was conducted across all administrative regions of mainland China to assess the validation status of ESs. Fifty-eight cities were selected with stratification by municipality, provincial capital, and other cities, and health care institutions and households in each city were chosen by convenience to identify ES devices in use according to the study protocol. Information on devices’ model, type, was collected. The validation status of each device was ascertained through searching international online registries of its models.

RESULTS:

A total of 3230 ES devices (1924 from health care institutions and 1306 from households), encompassing 498 ES models, were actually selected. The weighted proportion of accuracy-validated devices was significantly higher in health care institutions (33.9% [95% CI, 31.8–36.0%]) compared with households (23.0% [95% CI, 20.8–25.4%];
P
<0.001), and both exceeded the proportions for models (17.7% [95% CI, 13.7–22.6%] and 12.5% [95% CI, 9.5–16.3%], respectively). Regional economic development, device type, and years in use were associated with the proportion of accuracy-validated devices. Notably, 8.8% of devices used in secondary hospitals were wrist type.

CONCLUSIONS:
The accuracy validation status of ESs in China in the number of devices in use shows a more favorable situation than that in the number of models but remains low and concerning. Policies are urgently needed to promote the widespread adoption of accuracy-validated models among all users.
Ayurvedic Management of Sciatica with Medicated Enema and Bloodletting: A Case Report
Authors: Basnal Pooja , Borkar Preeti , Manda Sonia , Pal Pratyendra 
Published: January 2025

Background: The most common condition that restricts movement is low back pain, which is particularly common in the most productive years of life. Sciatica syndrome accounts for 40% of patients’ severe lower back pain. Psychological strain places excessive strain on the spinal column and is a major contributing factor to the development of sciatica and lower back pain. Purpose: The illness indicated in Ayurveda has symptoms and signs similar to “Sciatica” in modern, contemporary medicine. In clinical practice, Gridhrasi (Sciatica), the conditions stated in Ayurveda are comparable to those treated in contemporary medicine. Methods: A 38-year-old female patient suffering from the above ailments, having pain in the lumbar region radiating pain to the left leg, tingling sensation in both legs, difficulty in walking, irregular sleep, and limping gait, was admitted to an Ayurvedic hospital. Her treatment comprised external and internal, consisting of Snehan with Vishgarbha Taila, Kati Basti with Mahamasadi Taila, and Kala Basti with Panchtiktaksheer (medicated enema). Results: These treatments had a very favorable outcome, it was discovered. Following therapy, there was a significant improvement in walking time, lower extremity motions, and pain alleviation. Conclusion: This method of treating Sciatica may be helpful in clinical settings and future research.
Analysis of macular retinal thickness in polyarteritis nodosa using spectral domain optical coherence tomography
Authors: Yang Che-Ning , Chen Chia-Ping , Hsieh Yi-Ting 
Published: January 2025

Abstract

Purpose
To identify the macular retinal layer thickness changes in polyarteritis nodosa (PAN) patients without pathological findings appearing in color fundus photography (CFP), and to investigate the correlations with disease durations.

Methods
A total of 24 PAN patients who had been for 3 years or more and underwent SD-OCT were recruited from the UK Biobank, with exclusions for diabetes, eye disease, or abnormal CFP findings. Only the right eyes were included, with each PAN patient paired one-to-one with a control matched for age, sex, and ethnicity. Paired t-tests or Wilcoxon Signed-Rank tests were used to assess the differences in thickness of different retinal layers between groups, followed by linear regression analysis to evaluate the correlations with disease durations.

Results
PAN patients had significantly thinner retinal nerve fiber layer (RNFL) by 12.27% (mean ± standard deviation = 27.39 ± 8.94 μm for PAN patients and 31.22 ± 5.57 μm for controls, p = 0.048) and thinner outer plexiform and outer nuclear layers (OPL-ONL complex) by 10.67% (44.93 ± 6.59 μm for PAN patients and 50.31 ± 7.60 μm for controls, p = 0.032). Visual acuity and the whole macular thickness showed no statistical difference. The RNFL was thinned by 1.22 μm per year of disease progression (95% confidence interval: 0.12, 2.32, p = 0.042).

Conclusions
PAN patients without visual impairments or abnormal CFP findings may exhibit significant thinning in RNFL and OPL-ONL complex. SD-OCT may serve as a useful tool for early screening of ophthalmic changes in PAN.