bluuebunny/crossref_metadata_2025_split huggingface dataset:
GPCR Allostery: A View from Computational Biology
Authors: Li Mengrong , Bao Yiqiong , Li Miaomiao , Guo Jingjing 
Published: January 2023

Abstract:
G protein-coupled receptors (GPCRs) represent a large superfamily of cell-surface
proteins that mediate cell signaling and regulate virtually various aspects of physiological
and pathological processes, therefore serving as a rich source of drug targets. As
intrinsically allosteric proteins, numerous functions of GPCRs are regulated via allostery,
whereby allosteric modulators binding at a distal site regulate the function of the typical
orthosteric site. However, only a few GPCR allosteric ligands have been presently approved
as drugs due to the high dynamic structures of GPCRs. Fortunately, the rapid development
of computational biology sheds light on understanding the mechanism of
GPCR allosteric ligands, which is critical for the discovery of new therapeutic agents.
Here, we present a comprehensive overview of the currently available resources and approaches
in computational biology related to G protein-coupled receptor allostery and
their conformational dynamics. In addition, current limitations and major challenges in
the field are also discussed accordingly.
Exploration and Practice of Medicine Pathway for Perioperative VTE Prophylactic
Anticoagulant Treatment
Authors: Li Jing , Zhang Bin , Zhang Chuanzhou , Li Yongxin , Zhou Changkai , Shi Shiqiang , Ji Hongyan 
Published: January 2023

Objective:
Using perioperative VTE prophylactic anticoagulant pathway delicacy management, we
aimed to explore the role of clinical pharmacists in perioperative VTE prophylactic anticoagulant treatment and
the effect of the clinical medicine pathway implementation.

Methods:
In compliance with evidence-based medicine, combined with clinical practice, clinical pharmacists
participated in the formulation of the perioperative anticoagulant clinical medicine pathway. The PDCA cycle
was strictly implemented. Urologic Department, Affiliated Hospital of Qingdao University, Qingdao Shandong,
China, served as a pilot department for the evaluation of the effect of drug clinical pathway implementation.

Results:
The anticoagulant clinical medicine pathway for the urologic department was formulated and implemented
from October 2020 to August 2021. The entry rate gradually increased, and the accuracy of thrombosis
risk assessment improved. The proportion of drugs in the department and the per capita drug cost decreased significantly.

Conclusion:
The perioperative VTE prophylactic anticoagulant pathway showed significant achievements in
the rational use of urologic anticoagulant drugs, lowering the cost of medical care. Therefore, it could be considered
a novel long-term mechanism for rational analgesia drug use.
Therapeutic Potential of Herbal Medicine against Non-alcoholic Fatty
Liver Disease
Authors: Avan Amir , Pourali Ghazaleh , Hosseini Zeinab S. , Maftooh Mina , Nazari Elham , Khazaei Majid , Nassiri Mohammadreza , Hassanian Seyed M. , Ghayour-Mobarhan Majid , Ferns Gordon A. , Kiani Mohammad A. 
Published: January 2023

Abstract:
Non-alcoholic fatty liver disease (NAFLD) is a common metabolic disorder associated
with obesity, diabetes mellitus, dyslipidemia, and cardiovascular disease. A “multiple hit” model
has been a widely accepted explanation for the disease's complicated pathogenesis. Despite advances
in our knowledge of the processes underlying NAFLD, no conventional pharmaceutical
therapy exists. The only currently approved option is to make lifestyle modifications, such as dietary
and physical activity changes. The use of medicinal plants in the treatment of NAFLD has recently
gained interest. Thus, we review the current knowledge about these agents based on clinical
and preclinical studies. Moreover, the association between NAFLD and colorectal cancer (CRC),
one of the most common and lethal malignancies, has recently emerged as a new study area. We
overview the shared dysregulated pathways and the potential therapeutic effect of herbal medicines
for CRC prevention in patients with NAFLD.
Feasibility of Imaging-Based Quantitative Hepatic Function Assessment:
Gd-EOB-DTPA-Enhanced Liver MR Imaging Combined with T1 Mapping
Authors: Dong Zhi , Wang Meng , Feng Yanqing , Cai Huasong , Feng Shi-Ting , Li Zi-Ping , Lin Zhi , Peng Zhenpeng 
Published: January 2023

Objective:
to investigate the feasibility of gadoxetic acid (Gd-EOB-DTPA) enhanced MRI
combined with T1 mapping in quantitative hepatic function assessment.

Methods:
this study retrospectively enrolled 94 patients with Gd-EOB-DTPA enhanced MRI combined
with T1 mapping, divided into group A (grade A, n=73), group B (grade B, n=14) and group C
(grade C, n=7) based on Child-Pugh classification. Liver T1 relaxation times on plain scan (T1P) and
hepatocellular phase (T1E) were measured. Decrease in T1 (T1D) and the percentage of decrease in T1
(T1D%) were calculated as follows: T1D=T1P-T1E, T1D%= T1D/T1P×100%. The relationship between
T1P, T1E, T1D, T1D% and liver function classification was analyzed.

Results:
T1P, T1D, and T1D% in group A were significantly higher than those of group B and C. T1E in
group A was lower than those of group B and C. T1D% was significantly different between group B
and C. There was no significant difference in T1P, T1E, T1D between groups B and C. T1E was positively
correlated with liver function levels, T1P and T1D had a negative correlation with liver function levels.
T1P, T1E, T1D, T1D% were significantly different between cirrhotic and non-cirrhotic groups.
T1D% of less than 70% suggests liver dysfunction.

Conclusion:
Gd-EOB-DTPA enhanced liver MRI combined with T1 mapping is feasible for quantitative
assessment of hepatic function.
Primary Epiploic Appendagitis: Not Rare But Rarely Diagnosed Pathology
Authors: Kahveci Servet , Zitouni Soubhi , Abubakar Abdul Razak , İkizceli Turkan , Ozturk Mehmet , Aktürk Adem , Aksu Yusuf 
Published: January 2023

Objective:
The aim of this study is to determine the clinical and laboratory parameters
which may be suggestive of or even pathognomonic for primary epiploic appendagitis (PEA) and to
discuss the diagnostic efficacy of ultrasound (US) compared to computed tomography (CT) in patients
with PEA.

Materials and Methods:
For this retrospective study, 92 patients diagnosed with PEA using US, CT or
both modalities were included. All patient symptoms, clinical findings and laboratory parameters were
reviewed. The CT and US images of the PEA were evaluated for lesion size and location, the relationship
of the lesion to the colon and the distance of the lesion to the skin.

Results:
There were 16 female and 76 male patients in the study group. The mean age was 35 years
(range: 38–79 years). Well-localized abdominal pain was the primary symptom in all patients. The
mean leukocyte count was 7857±1326 mm-3. The most frequent localization of PEA was sigmoiddescending
colon junction (79/92). In patients who were examined by both US and CT, the size of the
fatty central core was between 15-48 mm (mean:28.10 mm) and 9-22 mm (mean:15.07 mm) in its
long-axis and short-axis diameter, respectively on US, whilst that by CT was between 15-46 mm
(mean:26.88 mm) and 9-21 mm (mean:14.40 mm) in its long-axis and short-axis diameter, respectively.
In patients who were examined by both US and CT, the mean distance of the lesions to the skin was
20.80 mm and 33.97 mm, respectively. All patients were treated conservatively with complete resolution
of symptoms within a week of presentation.

Conclusion:
PEA is an unrare self-limiting condition that should be considered in the differential diagnosis
of acute abdomen. To support clinicians and radiologists regarding PEA and its clinical, laboratory
and radiological findings, targeted sonographic examination - which is radiation and contrast
agent-free - could be highly sufficient for the diagnosis of PEA and may prevent unnecessary further
imaging and mistreatment.
Application of Microwave and Ultrasonication for Anthocyanin Extraction
Process from Garcinia indica Choisy Fruit Waste and Assessment of
Antioxidant Activity using Caenorhabditis elegans Model
Authors: Shastry Rajesh Padumane , Sriramareddy Srinath Boreddihalli 
Published: January 2023

Abstract:
Garcinia indica (also known as kokum) is a small evergreen tree that has been used in a
variety of culinary, industrial, and pharmacological products, as well as fruit juices and food. In the
present study, the antioxidant capacity of anthocyanin extracted from Garcinia indica fruit waste
was assessed using DPPH, ABTS assay, and a Caenorhabditis elegans infection model. The
independent variables, such as temperature, solvent concentration, microwave exposure, and
exposure to ultrasonication were integrated as independent variables in a five-level central
composite design using response surface methodology. Based on statistical analysis, the generated
models were successfully utilised to analyse the experimental data and determine the best
extraction conditions. The rescue effect of anthocyanin was further studied using a paralysis and
killing assay in a C. elegans infection model. The extraction yield was 21.0 mg/g under these
conditions, with antioxidant activity of 9.9 μg/ml by ABTS assay and 6.6 μg/ml by DPPH assay,
respectively. Furthermore, as compared to ethanol leaching extraction, this experimental design
increased anthocyanin yield by more than 15 fold. The treatment of anthocyanin with C. elegans
from E. coli and Pseudomonas aeruginosa PAO1 infection resulted in a significantly longer
lifetime. Garcinia indica fruit waste extracts high in anthocyanins might be employed as natural
food colorants and antioxidant additives in food products.
Identification of Novel Peptides as Potential Modulators of Aβ42 Amyloidogenesis:
An in silico Approach
Authors: Kundal Kavita , Paramasivam Santhosh , Mitra Amit , Sarkar Nandini 
Published: January 2023

Aims:
Alzheimer's disease is a neurodegenerative disease for which no cure is available.
The presence of amyloid plaques in the extracellular space of neural cells is the key feature of
this fatal disease.

Background:
The proteolysis of Amyloid Precursor Protein by presenilin leads to the formation of
Amyloid-beta peptides (Aβ 42/40). Deposition of 42 residual Aβ peptides forms fibril’s structure,
disrupting neuron synaptic transmission, inducing neural cell toxicity, and ultimately leading to
neuron death.

Objective:
Various novel peptides have been investigated via molecular docking and molecular
dynamic simulation studies to investigate their effects on Aβ amyloidogenesis.

Methods:
The sequence-based peptides were rationally designed and investigated for their interaction
with Aβ42 monomer and fibril, and their influence on the structural stability of target proteins
was studied.

Results:
Analyzed docking results suggest that the peptide YRIGY (P6) has the highest binding
affinity with Aβ42 fibril amongst all the synthetic peptides, and the peptide DKAPFF (P12) similarly
shows a better binding with the Aβ42 monomer. Moreover, simulation results also suggest
that the higher the binding affinity, the better the inhibitory action.

Conclusion:
These findings indicate that both the rationally designed peptides can modulate amyloidogenesis,
but peptide (P6) has better potential for the disaggregation of the fibrils. In contrast,
peptide P12 stabilizes the native structure of the Aβ42 monomer more effectively and hence can
serve as a potential amyloid inhibitor. Thus, these peptides can be explored as therapeutic agents
against Alzheimer's disease. Experimental testing of these peptides for immunogenicity, stability
in cellular conditions, toxic effects and membrane permeability can be the future research scope of
this study.
Hypoxia and Hypoxia Mimetic Agents As Potential Priming Approaches to
Empower Mesenchymal Stem Cells
Authors: Yasan Goknur Topaloglu , Gunel-Ozcan Aysen 
Published: January 2023

Abstract:
Mesenchymal stem cells (MSC) exhibit self-renewal capacity and multilineage differentiation
potential, making them attractive for research and clinical application. The properties of MSC can vary
depending on specific micro-environmental factors. MSC resides in specific niches with low oxygen concentrations,
where oxygen functions as a metabolic substrate and a signaling molecule. Conventional
physical incubators or chemically hypoxia mimetic agents are applied in cultures to mimic the original
low oxygen tension settings where MSC originated.

:
This review aims to focus on the current knowledge of the effects of various physical hypoxic conditions
and widely used hypoxia-mimetic agents-PHD inhibitors on mesenchymal stem cells at a cellular and
molecular level, including proliferation, stemness, differentiation, viability, apoptosis, senescence, migration,
immunomodulation behaviors, as well as epigenetic changes.
Isolinderalactone Resistance to the Liver Injury Induced by Oxaliplatin in Rats
Through Inhibiting IL-6/STAT3 Signal Pathway
Authors: Lin Rongwei , Zhou Yang , Hu Chao , Yao Hongqing , Jin Wangxun 
Published: January 2023

Background:
Oxaliplatin (OXA) is easy to cause sinusoidal obstruction syndrome (SOS), leading to liver
injury. Isolinderalactone (ILL), one of the main components of Lindera aggregate, has been reported to have a protecting
effect on the liver. However, it is unclear whether ILL has a therapeutic effect on liver injury caused by OXA. This
study aims to determine the effect of ILL on the prevention and treatment of OXA-induced liver injury and to provide
a basis for the chemotherapy of gastrointestinal tumors.

Methods:
Intraperitoneal injection of folinic acid, 5-fluorouracil, and OXA was administered on the SOS rat model for
7 weeks. The indexes of liver function were measured by biochemical kit. The ratio of liver weight to body weight was
calculated. The pathological analysis of the liver was scored with the SOS scoring standard, fibrosis was evaluated
with a four-point scale. The expression of inflammation factors was detected by Real-Time PCR, and the related indexes
of IL-6/STAT3 were examined by Western blot analysis.

Results:
ILL down-regulated the portal vein pressure and alleviated the abnormal liver function of SOS rats and improved
the liver lesions. ILL inhibited the SOS by inhibiting IL-6/STAT3.

Conclusion:
ILL resistance to liver injury through inhibiting IL-6/STAT3 signal pathway.
Choking Susceptibility and the Big Five Personality Traits
Authors: Thiessen Burgandy , Sullivan Philip , Gammage Kimberley , Dithurbide Lori 
Published: January 2023

Background:
Choking susceptibility is the likelihood or potential of an individual choking under pressure. Choking susceptibility can be influenced by personality traits.

Objective:
The purpose of this study is to examine the differences between the Big Five personality traits on choking susceptible and choking non-susceptible individuals from a Canadian University using a cross-sectional design. It was hypothesized that choking susceptibility could be predicted by the Big Five personality traits.

Methods:
A protocol developed by Mesagno and colleagues, comprising a self-consciousness scale, sports anxiety scale, and coping style scale, was used to measure choking susceptibility. The protocol has only been used within athlete populations. This study is the first to use the choking susceptibility protocol outside of sports, specifically for undergraduate students (N = 177).

Results:
A logistic regression revealed that the personality traits could significantly predict choking susceptibility. Neuroticism was the sole significant predictor. Higher neuroticism values significantly increased the probability of an individual choking susceptible.

Conclusion:
According to the current study, neuroticism predicted choking susceptibility. Future research should address choking susceptibility in different contexts and more closely examine the relationship between choking susceptibility and actually choking under pressure.
Perceived Stress and Association with Sociodemographic, Interpersonal Relationship and COVID-19 Lockdown Related Stress in South Africa
Authors: Maepa Mokoena , Kleinhans Atholl , Lentoor Antonio G. 
Published: January 2023

Background:
COVID-19 pandemic was declared a global public health emergency in March 2020. South Africa, like many countries, was not spared from this pandemic. In March 2020, the president announced a nationwide lockdown with social restrictions aimed to curb the spread of the virus. Such lockdown restrictions disrupted the normal day to day life for South Africans, leading to personal stress.

Objective:
The current study aimed to explore perceived stress and lockdown related stress among South African adults during the first wave of COVID 19.

Methods:
This study was cross-sectional in nature, using a sample of 203 adult males and females who were selected using a convenience sampling method on online social media platforms such as WhatsApp and Facebook. Participants completed an online survey that assessed socio-demographic information, perceived stress and lockdown related stress.

Results and Discussion:
A significant statical difference between males and females regarding their experience of lockdown related stress t (281) = 1.35, p < .004 was found. Relationship status was also significant for lockdown related stress t (281) = -2.02, p< .001 and perceived stress t= (281) = -.08, p< .000. No significant statistical difference between young and older adults in their experience of lockdown related stress and perceived stress was found.

Conclusion:
Male participants reported more lockdown related stress, while female participants reported more perceived stress. There were no age differences in lockdown related stress and perceived stress. People without intimate relationships reported more lockdown related stress and perceived stress than people with intimate relationships.
Systematic Review and Meta-analysis of the Relationship Between Proton
Pump Inhibitors and Esophageal Adenocarcinoma
Authors: Darvishi Mohammad , Soureshjan-Heidari Saeid , Bidares Matin 
Published: January 2023

Introduction and Aim:
Esophageal adenocarcinoma (EAC) mortality continues to increase
across the world. This meta-analysis was aimed to investigate the relationship between proton
pump inhibitors (PPIs) and the risk of EAC.

Methods:
This meta-analysis was done as per the PRISMA checklist using relevant keywords. To
this end, an extensive search was done on 29/6/2022 in EMBASE, Web of Science (ISI), PubMed,
and Scopus. In this study, 95% confidence interval (CI) and standardized mean difference (SMD)
were used to estimate the overall effect size. Analysis of the odds ratio (OR) for EAC was done
using a random effects model.

Results:
A total of 20 studies were included in the review. Compared to the group that received
PPIs, the OR of EAC in the recipients of the PPIs group was obtained at 0.67 (95% CI = 0.39-1.29,
P = 0.240). The meta-regression, including year, follow-up time, study design, sample size, quality
of the study, study period, and geographical location, demonstrated no source of heterogeneity (P
> 0.10).

Conclusion:
No significant relationship was found between PPIs use and the risk of EAC. Accordingly,
PPIs do not have a protective or risk factor effect on EAC.
UCDM: A User-Centric Data Model in Power Blockchain
Authors: Lv ZhiXing , Yu Hui , Kang Kai , Li Teng Chang , Du Guo Li 
Published: January 2023

Background:
As innovative information technology, blockchain has combined the
advantages of decentralization, immutability, data provenance, and contract operation automatically,
which can be used to solve the issues of single point failure, high trading cost, low effectiveness,
and data potential risk in power trading. However, in the traditional power blockchain,
the design of functional components in blockchain, such as the data structure of the block, does
not take the actual features of power into account, thus leading to a performance bottleneck in
practical application. Motivated by business characteristics of power trading, a user-centric data
model UCDM in consortium blockchain is proposed to achieve efficient data storage and quick
data retrieval.

Methods:
The proposed UCDM is designed by considering the requirements of transaction retrieval
and analysis, thus supporting the requirements of concurrent data requests and mass data
storage. The ID of each user will independently form its own chain over the blockchain.

Results:
Compared with the traditional data model, the extensive experimental results demonstrate
that the proposed UCDM has shorter processing delay, higher throughput, and shorter response
latency, thus having practical value.

Conclusion:
UCDM is an effective solution to the transaction retrieval and analysis in power
blockchain. Furthermore, the participant of the blockchain network has a unique identity over the
world, which ensures high security during trading.
Iterative Development, Validation, and Certification of a Smartphone System to Assess Neonatal Jaundice: Development and Usability Study
Authors: Aune Anders , Vartdal Gunnar , Jimenez Diaz Gabriela , Gierman Lobke Marijn , Bergseng Håkon , Darj Elisabeth 
Published: January 2023

Background
Medical device development is an area facing multiple challenges, resulting in a high number of products not reaching the clinical setting. Neonatal hyperbilirubinemia, manifesting as neonatal jaundice (NNJ), is an important cause of newborn morbidity and mortality. It is important to identify infants with neonatal hyperbilirubinemia at an early stage, but currently there is a lack of tools that are both accurate and affordable.

Objective
This study aimed to develop a novel system to assess the presence of NNJ. The device should provide accurate results, be approved as a medical device, be easy to use, and be produced at a price that is affordable even in low-resource settings.

Methods
We used an iterative approach to develop a smartphone-based system to detect the presence of NNJ. We performed technical development, followed by clinical and usability testing in parallel, after which we initiated the regulatory processes for certification. We updated the system in each iteration, and the final version underwent a clinical validation study on healthy term newborns aged 1 to 15 days before all documentation was submitted for conformity assessment to obtain Conformité Européenne (CE) certification. We developed a system that incorporates a smartphone app, a color calibration card, and a server.

Results
Three iterations of the smartphone-based system were developed; the final version was approved as a medical device after complying with Medical Device Regulation guidelines. A total of 201 infants were included in the validation study. Bilirubin values using the system highly correlated with total serum or plasma bilirubin levels (r=0.84). The system had a high sensitivity (94%) to detect severe jaundice, defined as total serum or plasma bilirubin >250 µmol/L, and maintained a high specificity (71%).

Conclusions
Our smartphone-based system has a high potential as a tool for identifying NNJ. An iterative approach to product development, conducted by working on different tasks in parallel, resulted in a functional and successful product. By adhering to the requirements for regulatory approval from the beginning of the project, we were able to develop a market-ready mobile health solution.