bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring female genital self-image: a psychological and sociocultural perspective
Authors: Mohammed Ghada Farouk , Al-Dhubaibi Mohammed Saleh , AbdElneam Ahmed Ibrahim , Bahaj Saleh Salem , Al-Dhubaibi Ahmed Mohammed 
Published: January 2025

Abstract

Introduction
Female genital self-image (FGSI) refers to one’s perception of the external and internal appearance of female genitalia. FGSI is a multidisciplinary research area influenced by psychological, sociocultural, and clinical factors, influencing sexual satisfaction, desire, self-perception, and partner perception.

Objectives
The review aims to improve doctors’ understanding of genital self-image by incorporating psychological and sociocultural perspectives, thereby promoting a comprehensive and holistic approach.

Methods
A comprehensive search technique was used to obtain research papers from the following databases: PubMed (MEDLINE), Scopus, and Web of Science. Google Scholar was also manually searched. The search results were imported into Rayyan reference management and assessed using the established inclusion criteria. The quality of the articles was assessed using the appropriate JBI checklist (ie, according to the study design), and the JBI grades of recommendation were used to grade the evidence.

Results
Psychological theories like self-schema, body image, and female personal harm view offer insights into FGSI, but none provide a comprehensive explanation of subjective body image dissatisfaction.
Research indicates women often experience anxiety about discussing genitalia and accepting sexual attention, but limited literature on negative self-image causes. Interventions include sexological counseling, therapy, educational programs, and public statements. Understanding FGSI is crucial for effective pre-surgical counseling, incorporating online portrayals, attitudes, and reasons for cosmetic surgery, and addressing psychological and sociocultural factors.
This text delves into the intricate psychological and sociocultural factors that shape FGSI, presenting a model of intra- and interpersonal dynamics. The link between FGSI and negativism is unclear, but it is linked to women’s confidence and subjectivity, with positive self-perceptions predicting higher sexual satisfaction and mental health.

Conclusions
FGSI has an imperative role in healthy female sexual function. The study suggests an interdisciplinary approach to managing FGSI, integrating psychological and sociological approaches, to better understand women’s anatomy and address gendered distress.
Effects of milk replacer composition on growth and development of beef × dairy crossbred calves
Authors: Carter Rachel E , Emenheiser Joseph C , Zinn Steven A , Govoni Kristen E , Felix Tara L , Reed Sarah A 
Published: January 2025

Abstract
The production of crossbred beef × dairy (B × D) calves is increasing; however, evaluation of pre-weaning feeding strategies for B × D calves is limited. We hypothesized that both male and female B × D calves fed added fat and protein through milk replacer would have increased muscle growth, muscle fiber cross-sectional area, average daily gain, BW, and morphometric measurements. We also hypothesized that calves fed the additional fat and protein milk replacer would have upregulation of regulatory genes involved in muscle hypertrophy. SimAngus × Holstein calves (n = 42) were assigned to one of two milk replacers: 30.0% crude protein (CP), 32% crude fat (CF) milk replacer (HPHF, n = 11 males, 11 females), or 22% CP, 20% CF milk replacer (CON, n = 10 males, 10 females) from 0 to 8 wk of age. B × D calves were weighed at birth and weekly thereafter. At week 2 and 8, longissimus muscle biopsies were collected for muscle fiber cross-sectional area (CSA) or gene expression analysis. Ultrasounds were performed at 4 and 8 wk of age to quantify ribeye area (REA), and backfat and rump fat thickness. Morphometric measurements, BW, CSA, and ultrasound data were analyzed with PROC MIXED with animal as the subject and fixed effects of milk replacer, age, sex, and their interactions. Gene expression data were analyzed in R Studio. Calves that consumed the HPHF milk replacer were heavier than calves consuming the CON milk replacer (HPHF: 70.7 ± 0.39 kg; CON: 68.5 ± 0.41 kg; P &lt; 0.01). At 8 wk of age, HPHF calves tended to have 14% larger muscle fiber CSA than CON calves (P = 0.06). No differences due to diet were observed for REA or fat thickness (P ≥ 0.38). Expression of MyoD tended to be 34% greater in CON females than HPHF females at 2 wk (P = 0.06), but at 8 wk, HPHF females tended to express 39% more MyoD than CON females (P = 0.09). Myogenin expression was 3% greater in CON calves than HPHF calves at 2 wk (P = 0.02), and CON females tended to express 52% more IGF-1 than HPHF females (P = 0.07). Feeding a milk replacer with a protein and fat content similar to beef cow milk improves B × D calf growth compared with a conventional milk replacer with less protein and fat. Improvements in early growth may improve B × D carcass quality and quantity, with the potential to increase return to the producer.
Differential transcriptomic profiling of lipid metabolism and collagen remodeling in fast‐ and slow‐twitch skeletal muscles in aging
Authors: Liu Yujia , Xia Guofang , Zhu Simeng , Shi Yifan , Huang Xueping , Wu Jin , Xu Congfeng , Du Ailian 
Published: January 2025

AbstractSkeletal muscle function gradually declines with aging, presenting substantial health and societal challenges. Comparative analysis of how aging affects fast‐ and slow‐twitch muscles remains lacking. We utilized 20‐month‐old mice to reveal the aging effects on muscle structure and fiber composition, followed by bulk RNA sequencing for fast‐ and slow‐twitch muscles and integration with human single‐cell RNA sequencing dataset providing a comparative analysis across species. In mouse slow‐twitch muscles, aging induced a switch from fast to slow fibers and distinctively altered lipid metabolism in ceramide and triglyceride, with the upregulation of regulatory genes Gk and Ppargc1a also observed in human slow fibers. Additionally, both types of muscles exhibited common collagen deposition and fibrosis, possibly due to the imbalance between collagen synthesis and degradation. The extracellular matrix gene changes substantially overlapped between mice and humans in aging, yet also highlighted clear differences. This integrative analysis provides further understanding of aged fast‐ and slow‐twitch muscles and offers new insights into the molecular changes in aging.
Implementing Early Kangaroo Mother Care
Authors: Hull William L. , Gongora Priscilla 
Published: January 2025

Background:
Kangaroo Mother Care (KMC) has numerous maternal and neonatal benefits. KMC is often overlooked as a vital neonatal stabilization tool. Factors that influence the initiation of KMC by bedside staff include a lack of specific education on KMC, low confidence, and hesitancy due to the infant’s clinical illness. Parents can pick up on the staff’s hesitancy and may not realize they can request to hold their baby.

Purpose:
The purpose of this quality improvement (QI) project was to decrease the time to the first opportunity for eligible neonates for KMC within the first 24 hours.

Method:
The location of the project was in a 35-bed NICU, private, and semi-private room mix. The project leads revised, updated, and received facility approval for a KMC policy. Parent education was provided via a bi-lingual pamphlet and video. KMC Champions were utilized to increase early KMC rates.

Results:
During the 12th week of the 3-month monitoring period, all eligible infant dyads were held within the first 24 hours, achieving the goal of 100%. Offering various educational opportunities led to enthusiastic staff participation. Additionally, reinforcement of importance and exposure to KMC led to an increase in staff buy-in.

Implications for Practice and Research:
Increasing staff knowledge on the importance of KMC may lead to increased confidence and competence, and a potential decrease in apprehension in offering and performing KMC. Further research needs to explore and define causes of staff hesitancy that lead to a decrease in KMC opportunities.
Effects of dynamic resistance training on postexercise hypotension and its mechanisms in hypertensive men
Authors: Fecchio Rafael Yokoyama , Sousa Julio C.S. de , Oliveira-Silva Laura , Silva Junior Natan D. da , Pio-Abreu Andrea , Silva Giovânio V. da , Drager Luciano F. , Low David A. , Forjaz Cláudia L.M. 
Published: January 2025

Background
A possible chronic effect of exercise training is the attenuation of the acute decrease in blood pressure (BP) observed after the execution of a session of exercise [i.e. called postexercise hypotension (PEH)]. However, there are few empirical data regarding this issue, and the possible mechanisms involved in this blunted response have not been studied.

Aim
The study aimed to evaluate the effects of dynamic resistance training (DRT) on PEH and its systemic, vascular, and autonomic mechanisms.

Methods
Data from 16 middle-aged treated hypertensive men who underwent DRT (eight exercises, 50% of 1RM, three sets until moderate fatigue) three times/week for 10 weeks were analyzed. Before and after the training period, the participants underwent an experimental session in which BP (auscultation), systemic hemodynamics (CO2 rebreathing), vascular function (duplex ultrasound), and cardiovascular autonomic modulation (spectral analysis of heart rate and BP variabilities) were assessed before and after a session of DRT.

Results
DRT reduced preexercise systolic BP and mitigated the systolic PEH that occurred before but not after the training period (P = 0.017). DRT did not change the diastolic PEH that occurred with similar magnitude before and after the training period (P = 0.024). DRT did not change the PEH mechanisms, except for cardiac sympathovagal balance that increased significantly more after the session of DRT conducted in the posttraining evaluation (P = 0.017).

Conclusion
In medicated hypertensive men, 10 weeks of DRT decreased preexercise systolic BP, abolished systolic PEH, and induced a greater increase in postdynamic resistance exercise sympathovagal balance.
Validation of the Microlife BP3T01-1B blood pressure monitoring device in adults and adolescents according to the ISO 81060-2:2018 protocol
Authors: Botta Beate , Bramlage Carsten , Hachaturyan Violetta , Jost Lena , Bramlage Peter 
Published: January 2025

Background
Accurate measurement of blood pressure (BP) is crucial for the diagnosis and ongoing management of hypertension. Only devices that have been validated using an established protocol should be used to measure BP. The aim of this study was to validate the Microlife BP3T01-1B, an automated upper-arm BP measuring device, according to the DIN EN ISO 81060-2:2018-11 (ANSI/AAMI/ISO 81060-2:2018) standard protocol.

Methods
BP measurements were performed in 85 adults and adolescents (age range: 13–86 years) using the same-arm sequential method of the DIN EN ISO 81060-2:2018-11 protocol, alternating between the test device and a reference mercury sphygmomanometer.

Results
A total of 255 valid comparisons were available for the analysis of criterion 1. The mean ± SD difference between the test device and the reference device values was −2.56 ± 7.53 mmHg for SBP and −3.10 ± 5.65 mmHg for DBP. The mean differences met the pass criterion of less than or equal to ±5 mmHg and the SD values met the pass criterion of ≤8 mmHg. All 85 participants were included in the analysis of criterion 2. The mean ± SD intraindividual difference between the test and reference devices was −2.56 ± 6.15 mmHg for SBP and −3.10 ± 4.85 mmHg for DBP. Both were within the required pass range of SD ≤6.43 mmHg for SBP and SD ≤6.20 mmHg for DBP.

Conclusion
The Microlife BP3T01-1B BP monitoring device fulfilled the criteria of the DIN EN ISO 81060-2:2018-11 (ANSI/AAMI/ISO 81060-2:2018) protocol in a general population and can be recommended for the measurement of BP in adults and adolescents.
The future approach for the management of acute bacterial skin and skin structure infections
Authors: Tiseo Giusy , Falcone Marco 
Published: January 2025

Purpose of review
To discuss the new available options for the treatment of acute bacterial skin and skin structure infections (ABSSSIs) and how to implement in the clinical practice innovative approaches for their management.

Recent findings
The availability of long-acting antibiotics, including dalbavancin and oritavancin, changed the approach to patients with ABSSSI. Direct discharge from the emergency department and early discharge from the hospital should be considered in patients with ABSSSI. Despite limited data about different bactericidal properties, the choice between dalbavancin and oritavacin is usually based on patients’ characteristics and comorbidities. Delafloxacin and omadacycline are other options and have the advantage to be available for both intravenous and oral formulations, allowing a sequential therapy and switch from intravenous to oral treatment in clinically stable patients. Further studies should elucidate the profile of patients who may beneficiate from these drugs.

Summary
Early discharge from the hospital should be considered in patients with ABSSSI at a high risk of methicillin-resistant Staphylococcus aureus and in vulnerable patients for which hospitalization may have detrimental consequences. In elderly individuals, patients with diabetes mellitus, oncological people who need for continuing their healthcare pathway, this approach may reduce complications and costs related to hospitalization.
Computational Modeling of Ternary Complex Formation and Targeted Protein Degradation Mediated by Molecular Glues
Authors: Izaguirre Jesus A , Wu Yujie , McDargh Zachary , Palpant Timothy , Razavi Asghar M , Trovato Fabio , Koh Cheryl , Xu Huafeng 
Published: January 2025

AbstractWe introduce GlueMap, a computational toolkit to discover and optimize molecular glues. GlueMap integrates structural and pharmacodynamic modeling, molecular dynamics (MD), and machine learning (ML) for modeling targeted protein degradation (TPD) mediated by molecular glues (MGs). GlueMap accurately models the structural ensemble of ternary complexes, predicts their thermodynamic stabilities, and evaluates downstream effects such as ubiquitination and degradation efficiency. We validated GlueMap’s capabilities through two case studies: CRBN-dependent degradation of GSPT1 and DDB1-dependent degradation of CDK12. For CRBN-GSPT1, GlueMap successfully recovered known molecular glue degraders and identified novel candidates through prospective virtual screening, leveraging ternary complexes derived from extensive MD simulation. In the DDB1-CDK12 system, we observed strong correlations between ternary complex stability, quantified by binding free energy calculations, and degradation efficiency, though notable exceptions highlighted the importance of considering structural dynamics of the ternary complex in the context of the cullin ring ligase. Through a supervised variational autoencoder (VAE) model combined with attention-based regression, GlueMap demonstrates high accuracy in predicting degradation potency from structural features. By integrating structural, thermodynamic, and ubiquitination metrics, GlueMap provides a multi-criteria approach to rational MG design, distinguishing it from traditional screening methods that rely primarily on docking scores. These results illustrate that GlueMap may provide mechanistic insights and accelerate molecular glue discovery and optimization across diverse protein targets.
TRANSCRIPTOMIC ANALYSIS OF THE RELATIONSHIP BETWEEN PANCREATIC DUCTAL ADENOCARCINOMA STROMAL SUBTYPES AND NEURAL INVASION
Authors: Yildizhan Saliha E. , Demir Ihsan E. , Sever Elif Arik , Zeybek Didem Karakas , Sezerman O. Ugur , Ceyhan Güralp O. 
Published: January 2025

AbstractWe investigated the association between pancreatic ductal adenocarcinoma (PDAC) subtypes and neural invasion (NI) status in order to assess what factors influence the inter-tumor heterogeneity of NI. We built single-sample classifiers for prominent subtype schemes using multiple approaches. We predicted the subtype labels of samples with known NI status using the classifiers based on pairwise gene expression comparisons, which were consistently the best-performing models. These classifiers were made available in a Shiny app (https://salihaey.shinyapps.io/pcsubtypes/). Stromal subtypes were found to be most significantly associated with NI, with activated stroma being enriched in neuro-invasive samples. We compared the results of differential expression and gene set enrichment analyses between NI and stromal subtypes from both bulk and single-cell RNA-seq data, which revealed a prominent role of myCAFs and common signatures between activated stroma and NI (e.g., ECM remodeling and TGFβ signaling) as well as signatures that changed in divergent directions for activated stroma and NI (e.g., iCAF markers). Our study presents support for the clinical implications of PDAC stromal subtypes by revealing their association with NI incidence, while providing biological insights into the interactions between diverse genetic programs that influence NI emergence.
Fluorescence lifetime imaging microscopy for metabolic analysis of LDHB inhibition in triple negative breast cancer
Authors: Galloway A. , Hofstede B. Ter , Walsh Alex J. 
Published: January 2025

AbstractTriple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with no targeted treatments currently available. TNBC cells participate in metabolic symbiosis, a process that optimizes tumor growth by balancing metabolic processes between glycolysis and oxidative phosphorylation through increased activity by the enzyme lactate dehydrogenase B (LDHB). Metabolic symbiosis allows oxidative cancer cells to function at a similar rate as glycolytic cancer cells, increasing overall metabolic activity and proliferation. Here, fluorescence lifetime imaging microscopy (FLIM) is used to analyze the metabolism of TNBC cells with inhibition of LDHB using a multiphoton microscope to measure the fluorescent lifetimes of two metabolic coenzymes, NAD(P)H and FAD. LDHB is inhibited via an indole derivative known as AXKO-0046 in varying concentrations. Understanding how TNBC cell metabolism changes due to LDHB inhibition will provide further insight into metabolic symbiosis and potential new TNBC treatment options.
Deciphering Features of Metalloprotease Cleavage Targets Using Protein Structure Prediction
Authors: Chung Dae sun , Park Jongkeun , Choi Won Jong , Seong Do Young , Hong Dongwan 
Published: January 2025

AbstractMetalloproteases are a class of enzymes that utilize metal ions within their active sites to catalyze the hydrolysis of peptide bonds in proteins. Among these, ADAM10 (A Disintegrin and Metalloproteinase 10), a member of the ADAM family, plays a crucial role in mediating intracellular signaling by cleaving specific substrates, thereby influencing a variety of physiological and pathological processes. The mechanisms underlying the activity of ADAM10 present significant opportunities for the development of novel therapeutic strategies aimed at disease intervention. However, the information available to identify the substrate and cleavage sites of ADAM10 is still insufficient. Therefore, it is essential to identify and classify the features of substrates and to elucidate cleavage sites through experimental approaches. However, these studies across numerous proteins present significant challenges.To address the promise of these investigations, we developed a model that leverages protein structure prediction to decipher substrate features, classify substrates, and predict cleavage sites. Through the analysis of predicted protein complexes between ADAM10 and its substrates using PDB files, we evaluated protein-protein interaction (PPI) data, the structural information of cleavage sites, and the spatial relationships between the cleavage sites and metal ions.Finally, we present a computational model that effectively classifies substrates and accurately predicts cleavage sites in this study. Our study demonstrates the potential for application not only to ADAM10 but also to other members of the ADAM family and, more broadly, to additional metalloproteases. By leveraging computed protein structural information, our approach offers a novel perspective for substrate classification.
Testing for divergence in a plant symbiont across two natural environmental gradients
Authors: Chang Chang-Yu , Topping-Brown Terrence , Rud Jazmine L. , Calvert McCall B. , Bencosme Gerardo , Robinson Linda J. , Wood Corlett W. 
Published: January 2025

AbstractMicrobes dominate the biological diversity found in natural landscapes. Yet, studies of microbial population differentiation across environmental gradients have been surprisingly scarce. In this study, we tested for phenotypic and genetic divergence in rhizobia bacteria (genusSinorhizobium) associated with the legumeMedicago lupulinaacross two environmental gradients: an elevation gradient in the Appalachian Mountains and an urbanization gradient in Philadelphia. Common garden experiments inMedicago sativarevealed that symbiotic traits differed significantly between high- and low-elevation rhizobia populations, but not between urban and suburban populations. Nitrogen supplementation erased the trait differences between low- and high-elevation populations, consistent with the hypothesis that these differences arise from the nitrogen that rhizobia provide. In contrast to symbiotic traits, free-living growth traits showed minimal differentiation across gradients. To test the hypothesis that greater gene flow constrains phenotypic divergence in the urbanization gradient, we compared the strength of isolation by distance in the core and accessory genomes across the two gradients. Contrary to our expectation, isolation by distance was significantly stronger in the urbanization gradient in both the core and accessory genomes. These results suggest that our focal elevation gradient imposed stronger divergent selection on rhizobia than our focal urbanization gradient. Together, this study highlights the need for a holistic understanding of microbial evolution with regard to the traits across life stages, molecular evolution across genomic levels, and evolutionary processes across environmental gradients.