bluuebunny/crossref_metadata_2025_split huggingface dataset:
KEBERLANJUTAN KINERJA LINGKUNGAN: INVESTASI MASA DEPAN BERBASIS EKONOMI HIJAU BAGI NILAI PERUSAHAAN
Authors: Heriyah Heriyah 
Published: February 2025

Penelitian ini menegaskan bahwa kinerja lingkungan adalah elemen kunci dalam menciptakan nilai perusahaan yang berkelanjutan. Perusahaan yang mengintegrasikan prinsip-prinsip ekonomi hijau dalam operasionalnya tidak hanya meningkatkan daya saing tetapi juga menarik investasi yang lebih besar dan memberikan dampak positif bagi lingkungan. Dengan semakin meningkatnya perhatian global terhadap isu lingkungan, strategi berbasis keberlanjutan menjadi kebutuhan mendesak. Penelitian ini dilakukan pada 165 perusahaan manufaktur yang terdaftar di Bursa Efek Indonesia selama periode 2019 hingga 2023. Penelitian menggunakan pendekatan kuantitatif dengan menggunakan SPSS serta melakukan analisis regresi. Penelitian berkontribusi pada analisis keuangan dimana hasil penelitian diperoleh bahwa meskipun struktur modal dan kebijakan dividen tidak secara signifikan memengaruhi nilai perusahaan, pertumbuhan perusahaan, profitabilitas, dan keputusan investasi memberikan pengaruh yang signifikan. Ke depan, penelitian lebih lanjut diperlukan untuk mengeksplorasi pengaruh regulasi pemerintah, tren pasar, dan faktor eksternal lainnya terhadap hubungan antara keberlanjutan dan nilai perusahaan. Pendekatan holistik ini dapat membantu perusahaan dalam menciptakan keseimbangan antara pertumbuhan ekonomi, keberlanjutan lingkungan, dan kesejahteraan sosial.
Hydrogel and scalp/skin conductivities impact dose from tumor treating fields
Authors: Lok Edwin , Liang Olivia , Haack Monika , Wong Eric T. 
Published: February 2025

PurposeTumor Treating Fields (TTFields) are delivered by transducer arrays applied to scalp or body surface for treatment of multiple malignancies. Dermatologic complications are thought to be related to hydrogel situated between the electrodes and scalp or skin to facilitate electric field penetration. High intensity of TTFields on these surfaces may also be a contributing factor. We explored conductivity changes in the hydrogel and skin to improve TTFields coverage and penetration.MethodsMagnetic resonance imaging datasets from 12 glioblastoma patients and attenuation-corrected positron emission tomography–computed tomography datasets from 3 non-small cell lung and 2 ovarian carcinoma patients were used to segment anatomic structures. Finite element mesh models were generated and solved for distribution of applied electric fields, rate of energy deposition, and current density at the gross tumor volume (GTV) and clinical target volume (CTV). Electric field-volume, specific absorption rate–volume, and current density-volume histograms were generated, by which plan quality metrics were used to evaluate relative differences in field coverage between models at various hydrogel and skin conductivities.ResultsBy varying conductivity of hydrogel, TTFields coverage at GTV or CTV increased up to 0.5 S/m for head and 1.0 S/m for thorax and pelvis models, and no additional increase was observed beyond these saturation points. Although scalp hotspots increased or decreased by +1.5%, −0.1%, and −0.9% in E5%, SAR5%, and CD5%, the skin hotspots increased by as much as +23.5%, +45.7%, and +20.6%, respectively. When altering conductivity of the entire scalp, TTFields coverage peaked near 1 S/m at the GTV or CTV for the head models. TTFields coverage in both the GTV and scalp increased up to 1 S/m for the head models but plateaued thereafter. Contouring under the scalp increased scalp hotspots by +316% in E5% at 1 S/m compared to altering the conductivity of the entire scalp. GTV hotspots decreased by +17% in E5% at 1 S/m.ConclusionTTFields delivery can be modulated by the conductivity of hydrogel and scalp/skin at the transducer-scalp or transducer-skin interface. Optimizing this aspect of TTFields delivery may increase tumor control while minimizing toxicity at the scalp or skin.
Selenium-modified hydroxyapatite titanium coating: enhancing osteogenesis and inhibiting cancer in bone invasion by head and neck squamous cell carcinoma
Authors: Wen Xutao , Zhou Qin , Lin Sihan , Mai Huaming , Zhang Ling 
Published: February 2025

IntroductionHead and neck squamous cell carcinoma (HNSCC) frequently invades the jaw, and surgical treatment often leads to bone defects requiring reconstruction with titanium plates. To enhance the anti-tumor and bone regeneration properties of titanium, a selenium-modified hydroxyapatite coating was developed on titanium surfaces.MethodsSelenium-modified hydroxyapatite coatings was fabricated using micro-arc oxidation (MAO). The coating properties were characterized by SEM, XPS, AFM, Contacting angle test and ICP-OES. Cell proliferation assays were performed using rBMSCs and Cal27 cells. The osteogenic potential of the materials was assessed via ALP and OCN immunofluorescence staining and quantitative polymerase chain reaction (qPCR). Apoptosis in Cal27 cells was analyzed through flow cytometry, and ROS levels in rBMSCs and Cal27 cells were measured using ROS fluorescent probes.ResultsA coating was successfully formed on the surface of titanium with a porous structure via MAO. The atomic percentages of calcium, phosphorus and selenium on the coating surface were 42.47%, 45.43% and 12.3%, respectively, and the ion components could be released steadily and slowly. In vitro, 0.2 µg/mL selenium had toxic effects on Cal27 and promoted osteogenic differentiation of rBMSCs. PCR showed that selenium increased the expression of genes related to osteogenic differentiation of rBMSCs by 3–5 times. ROS detection found differences in intracellular ROS content between Cal27 and rBMSCs.DiscussionBy incorporating selenium-modified coatings, titanium implant materials can simultaneously promote osteogenesis and inhibit tumor growth, offering a promising strategy for postoperative functional recovery in HNSCC patients.
Biological boundary conditions regulate the internalization of Aspergillus fumigatus conidia by alveolar cells
Authors: Schiefermeier-Mach Natalia , Polleux Julien , Heinrich Lea , Lechner Lukas , Vorona Olexandra , Perkhofer Susanne 
Published: February 2025

IntroductionThe lung environment is defined by unique biological boundary conditions, including complex alveolar geometry, extracellular matrix composition and mechanical forces generated during respiration. These factors were shown to regulate alveolar permeability, surfactant secretion, cell contractility and apoptosis, but their role in fungal infections remains unknown. Aspergillus fumigatus is a critical fungal pathogen that causes severe pulmonary infections in immunocompromised individuals. Our study addresses a knowledge gap by investigating how boundary conditions affect A. fumigatus conidia interactions with alveolar epithelial cells.MethodsWe applied micropatterned substrates to confine cells into defined shapes and densities, allowing precise control over geometric conditions and extracellular matrix composition. Using cell line stably expressing the phagolysosomal protein Lamp1-NeonGreen and multiplane fluorescent microscopy, we evaluated A. fumigatus conidia binding and internalization efficiency.ResultsWe observed significantly faster and more efficient A. fumigatus conidia internalization in cells confined on micropatterns compared to previously reported studies using cell monolayers. Altering cell geometry, density and extracellular matrix composition strongly affected conidia binding and localization to Lamp1+ phagolysosomes. Cells on X-shaped or multicellular micropatterns showed higher internalization rates, particularly at the periphery, suggesting spatial heterogeneity in pathogen uptake. Additionally, changes in extracellular matrix composition influenced the intracellular trafficking of A. fumigatus conidia.DiscussionOur findings emphasize the essential role that local mechanical and biochemical cues play in shaping the interactions between fungal pathogens and alveolar cells. Understanding how lung boundary conditions change in disease states will provide important insights into fungal infection outcomes.
Association between RC/HDL-C and hyperuricemia in adults: evidence from NHANES 2005-2018
Authors: Tai Yanghao , Chen Bin , Kong Yingming , Wang Xuening 
Published: February 2025

BackgroundThe incidence of hyperuricemia is growing in the world, with a significant influence on the survival and healthy condition of the patient. The connection between serum residual cholesterol (RC) to high-density lipoprotein cholesterol (HDL-C) ratio and hyperuricemia is uncertain. Consequently, we tried to elucidate the connection between the hyperuricemia and RC/HDL-C ratio.MethodsBased on the National Health and Nutrition Examination Survey (NHANES) database, data from 2005 to 2018 were utilized in this cross-sectional research. RC/HDL-C index was calculated by (TC - HDL-C - LDL-C)/HDL-C. Participants were diagnosed with hyperuricemia when the serum uric acid concentration reached 6 mg/dL in women and 7 mg/dL in men. Our researcher utilized smoothed curve fitting and multivariate logistic regression analysis to examine between RC/HDL-C and hyperuricemia among adults. The consistency of these results was examined in various population subgroups.Results2376 individuals (19.1%) were stratified into the hyperuricemia group. We observed statistically significant differences (P values &lt; 0.05) in the hyperuricemia population for remaining variables, except for economic level and alcohol drinking. After correcting for potential confounders, our researchers discovered the strong positive connection between the RC/HDL-C and the possibility of incurring hyperuricemia. The incidence of RC/HDL-C elevated by 98% with each additional unit of the RC/HDL-C. Subgroup analyses showed correlations for the majority of subgroups remained stable. However, gender and several diseases may modify this association.ConclusionsHigher RC/HDL-C is correlated with higher prevalence rate of developing hyperuricemia. However, further research is still required to confirm the causal association.
Biochemical remission, diagnostic delays, and comorbidities of acromegaly in China: a large single-centre retrospective study
Authors: Bai Xue , Duan Lian , Yang Shengmin , Wang Tingyu , Yao Yong , Zhang Meng , Zhou Jingya , Cui Shengnan , Pang Cheng , Wang Yi , Zhu Huijuan 
Published: February 2025

IntroductionLong-term biochemical nonremission and long-delayed diagnosis can increase the incidence of comorbidities of acromegaly and seriously affect patients’ quality of life. To identify predictors of biochemical remission and quantify the relationship between delayed diagnosis and comorbidities, we performed a retrospective study of a large, single-centre cohort.MethodsThis retrospective cohort included 1692 hospitalised patients with acromegaly seen in a single referral centre between 2012 and 2020. To account for the longitudinal data structure, generalised estimating equation (GEE) regression models were established to further evaluate the factors associated with biochemical remission.ResultsOverall, 1692 inpatients (55.4% females, mean age at diagnosis: 40.1 ± 12.2 years, mean age at onset: 34.4 ± 11.71 years, median diagnostic delay: 4.4 years) were included. A total of 86.8% (1306/1504) had macroadenomas, and 34.1% (486/1424) had invasive tumours. According to the international diagnostic criteria, the 5-year biochemical remission rate of this cohort was 26.4%, while the Chinese criterion was 41.4%. According to the GEE model, invasion and large adenoma influence biochemical nonremission. After age 50, comorbidities such as hypertension and hyperlipidaemia were considerably more common in females than in males. The proportion of patients with comorbidities among those with a delayed diagnosis ≥4 years was greater than among those with a delayed diagnosis &lt;4 years (54.9 vs. 47.9%, P=0.004).ConclusionThe older the age at diagnosis and the longer the delay in diagnosis, the greater the incidence of comorbidities, especially in elderly females. Appropriate treatment of acromegaly should be started early to achieve biochemical control.
Prevalence and associated factors of recent HIV-1 infection among newly identified HIV-positive individuals tested with the Asante Rapid Recency assay in Harari region, Eastern Ethiopia
Authors: Nure Muzemil Ebrahim , Weldegebreal Fitsum , Tebeje Fikru , Sime Akewok , Regassa Lemma Demissie 
Published: February 2025

BackgroundHuman immunodeficiency virus (HIV) is a virus that attacks the immune system. Globally, more than 79.3 million people have been infected with it, and about 36.3 million people have died since the beginning of the epidemic. Ethiopia is one of the major affected countries in sub-Saharan Africa, with a huge number of people living with HIV. The identification of recent HIV-1 infections plays a crucial role in guiding prevention and control interventions. Moreover, data on the prevalence and factors associated with recent HIV-1 infection among cases tested by the Asante Rapid Recency Assay at health facilities in the Harari region has been inadequate. This study aimed to assess the prevalence and associated factors of recent HIV-1 infection among newly identified HIV-positive individuals tested with the Asante Rapid Recency Assay in Health Facilities of Harari Region, Eastern Ethiopia.MethodsRetrospective cross-sectional study was employed using HIV-1 diagnoses data from April 15–20, 2024 with 580 study participants. The data were extracted based on the standardized HIV Case-Based Surveillance report form, as outlined by the Ethiopian Public Health Institute. Data extracted from Redcap were checked and cleared for completeness then entered and analysed using the Statistical Package for Social Science software version 27. Bivariate and multivariable regression analyses were carried out to examine the associations between dependent and independent variables. A P-value of &lt;0.05 was considered statistically significant.ResultsThe overall prevalence of recent HIV infection was 9.1% (95% CI: 7.0%, 11.8%). The highest proportion of recent HIV infections was in the year 2019 [9(22.5%)]. The study also found that no formal education (AOR = 18.424, 95% CI = 1.468–231.2), primary educational level (AOR = 22.1, 95% CI = 1.91–256.1, P = 0.013), no formal education (AOR = 18.424, 95% CI = 1.468–231.2, P = 0.028), having sex in the last 12 months (AOR = 5.508, 95% CI = 2.167–15.7, P = &lt;.001), having sex with known/suspected HIV positive (AOR = 4.35, 95% CI = 1.455–13.04, P = 0.009) and Illicit drug use (AOR = 57.8, 95% CI = 16.19–207.5, P = &lt;.001) had higher likelihood of having recent HIV infection.ConclusionThis study found a 9.1% proportion of recent HIV infections, indicating significant ongoing HIV transmission within the community. The study also revealed multiple risk factors for recent HIV infection, including lower educational levels, recent sexual activity, sex with high-risk partners, and drug use. This study emphasizes the significance of improving targeted HIV preventive programs.
Analysis of influencing factors and establishment of prediction model for successful vaginal delivery after cesarean section
Authors: Zhang Hongxia , Yu Lin , Wei Songquan , Li Guiming 
Published: February 2025

ObjectiveTo explore the influencing factors of vaginal delivery after cesarean section, establish a predictive model, and identify potential factors for perinatal complications.Materials and methodsThis is a retrospective analysis of women who attempted a trial of labor after cesarean section(TOLAC) at the Third Affiliated Hospital of Guangzhou Medical University and subsequently gave birth in this hospital between 31 December 31 2017 and December 2023. Associations between maternal characteristics and success of TOLAC were assessed using univariate and logistic regression. A predictive model was developed and performance was assessed using the acceptor-operator curve (ROC).ResultsA total of 10,277 pregnant women with a history of previous cesarean section were identified during the observation period, 1,065 attempted TOLAC, which 839 were successful vaginal birth after cesarean (VBAC) and 226 failed vaginal trials. We have developed and validated a simple nomogram prediction model based on common antenatal predictors, which are independently associated with successful TOLAC, including maternal age, height, cervical Bishop score, estimated fetal weight, and use of oxytocin and artificial rupture of membranes to induce labor. The prediction model has been established and verified, and the model demonstrates good prediction efficiency, with an area under the ROC curve of 83.1%. Compared with the TOLAC-failure group and the ERCD group, the VBAC group had the lowest amount of bleeding in intrapartum and 24 h after delivery, puerperal infection, and uterine rupture. Nevertheless, the prevalence of placental abruption and the incidence of neonatal neonatal intensive care unit were higher in this cohort.ConclusionTOLAC is an important public health strategy in China. The results of our study can be used to improve counselling, reduce decision-making conflicts and increase the success rate of trials of vaginal delivery, ultimately improving the prognosis for mother and baby, by providing case-specific possibilities for counselling and management of women with a history of caesarean section and according to the characteristics of each pregnancy.
A comprehensive prognostic and immunological implications of Gremlin 1 in lung adenocarcinoma
Authors: Li Hongyan , Zhou Yang , Xiao Jiaqing , Liu Fang 
Published: February 2025

BackgroundLung adenocarcinoma (LUAD) is a prevalent form of lung cancer globally, known for its high invasiveness, metastatic potential, and notable heterogeneity, particularly in its response to immunotherapy. Gremlin 1 (GREM1) is implicated in tumor progression and poor prognosis in multiple cancers. However, GREM1’s specific role in LUAD remains unclear. This study systematically examines GREM1 expression in LUAD and its association with tumor progression, immune microenvironment, and prognosis.MethodsGene expression data from the TCGA and GSE31210 databases were analyzed using Weighted Gene Co-expression Network Analysis (WGCNA), GO and KEGG enrichment analyses. The prognostic value of GREM1 was evaluated through survival analysis, Cox regression, and Kaplan-Meier curves. Additionally, immune microenvironment analysis was conducted to explore the relationship between GREM1 and immune cell infiltration. In vitro experiments, including Western blot and assays for cell proliferation, migration, and invasion, were performed to confirm the specific role of GREM1 in LUAD cells.ResultsGREM1 was significantly upregulated in tumor tissues and correlated with poor prognosis. Moreover, GREM1 was significantly associated with immune cell infiltration and immunotherapy response within the immune microenvironment. In vitro experiments confirmed that GREM1 overexpression significantly promoted LUAD cell proliferation, migration, and epithelial-mesenchymal transition (EMT), whereas GREM1 knockdown suppressed these functions.ConclusionsA comprehensive analysis indicates that GREM1 is crucial in LUAD progression, with its overexpression predicting poor prognosis. GREM1 could be a potential therapeutic target for LUAD, providing insights for personalized therapy optimization.
A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers
Authors: Pan Xiaolan , Wei Caibiao , Su Jingyu , Fang Min , Lin Qiumei , Qin Yuling , Gao Jie , Zhao Jie , Zhao Huiliu , Liu Fengfei 
Published: February 2025

Backgroundnuclear-associated antigen Ki67 (Ki67) emerges as a clinically practical biomarker for proliferation assessment among many cancer types. However, the definite prognostic value of Ki67 against a specific cancer type has remained vague. This study aims to perform a comprehensive pan-cancer analysis of the prognosis value of Ki67 across various cancer types.MethodsThis study explored the expression, prognostic value, and tumor-infiltrating immune of MKI67 in the TCGA database by pan-cancer, and then performed immunohistochemical, correlation analysis and prognostic analysis using 10028 patients of the top 10 cancer patients in China we collected. The correlation between MKI67 expression and survival outcome, clinical features, MSI, TMB, and tumor-infiltrating immune cells by TCGA database, xCell, and TIMER algorithms.ResultsMKI67 expression was significantly upregulated across varied cancer types verified by datasets. We found MKI67 expression was significantly associated with poor prognosis in LUADLUSC, LIHC, and BRCA patients, but good prognosis in COADREAD and READ patients via Kaplan-Meier survival analysis using 10028 patients collected. These results of our validation were generally consistent with TCGA database except BRCA, COADREAD and READ. Meanwhile, upregulation of MKI67 elevates the degree of immune infiltration of several immune cell subtypes, such as functional T cells, CD4+ T cells, and CD8+ T cells, as well as, MKI67 was related to Cell cycle, Oocyte meiosis, p53 and other pathways.ConclusionOur comprehensive analysis may supply useful guidance on MKI67 applicability across various cancer types. These observed results contribute to the promise of MKI67 in a realistic clinical setting and improve the outcomes of cancer patients.
The changes of NLRs family members in the brain of AD mouse model and AD patients
Authors: Li Zehan , He Yanling , Zhang Jingdan , Yang Jing , Cheng Jinbo , Zhang Xuewu 
Published: February 2025

IntroductionAlzheimer’s disease (AD), a prevalent neurodegenerative disease, is primarily characterized by progressive neuron loss and memory impairment. NOD-like receptors (NLRs) are crucial for immune regulation and maintaining cellular homeostasis. Recently, NLRs have been identified as important contributors to neuroinflammation, thus presenting a potential approach for reducing inflammation and slowing AD progression.MethodsWe use quantitative RT-PCR to detect levels of NLR family members in AD mouse model. Additionally, we use immunofluorescence to detect NLRP3 expressions in microglia surrounding Aβ plaques in AD mouse model and human AD patients.ResultsIn this study, we examined the expression of NLR family members in the human AD database, and found increased levels of CIITA, NOD1, NLRC5, NLRP1, NLRP3, NLRP7, NLRP10, NLRP12, and NLRP13 in hippocampus tissue in patients with AD, along with increased levels of NOD1, NLRC5, NLRX1, NLRP3, and NLRP7 levels in frontal cortex tissue. Furthermore, through detecting their levels in AD mouse model, we found that NLRP3 levels were significantly increased. Additionally, we found that NLRP3 expressions were mainly elevated in microglia surrounding Aβ plaques in AD mouse model and human AD patients.DiscussionThese findings highlight the potential important role of NLRP3 in AD pathology, offering new therapeutic targets and interventions.