bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design and modeling of a wearable articulated manipulator to assist the elderly grasp
Authors: Lu yishanchun , Li hongshuang , Liu manchang , Li yiming 
Published: February 2023

Abstract
Purpose –With the weakening of the body of the elderly, hand strength has decreased significantly, unable to adapt to daily life, some elderly people with Parkinson’s syndrome or some hand tremors caused by other diseases, and even will have trouble drinking water and eating daily small things. In order to solve the problem of grasping objects in daily life of the elderly with weak hand strength and some diseases, a wearable articulated manipulator that assists the elderly to grasp is designed.
Design/methodology/approach –The design is divided into four parts: wrist part, back part of the hand, finger part and drive part. For the elderly with trembling hands, the degree of freedom is reduced at the joint point of finger movement, and the use of underdrive mechanism can make the knuckles of the mechanical finger fit the shape of the object by themselves, and when taking irregular objects, the fingers will adaptively fit the outline of the object, ensuring the stability and safety of the overall grasping to the greatest extent.
Findings –Based on this design idea, a wearable articulated manipulator was designed, and mechanical analysis and kinematics analysis were carried out by designing and modeling the claw mechanism, determining the design size, and making the manipulator claw. Through the manipulator-assisted grasping and opening and lifting test tests, the practicality and safety of the designed assisted elderly grasping items are verified.
Originality/value –The morphological design of each finger is designed to fit the normal human hand, and each "joint" can be fine-tuned to adapt to the finger length of different elderly people. Each finger is biomimetically designed to perfectly conform to the human skeletal structure, while the interior is covered with flexible materials to make it more comfortable for the elderly to wear. And it has stable grasping ability and adjustment ability, reflecting good adaptability and suitable for the elderly.
Stochastic Modeling of the Theis Equation via Karhunen–Loeve and Polynomial Expansions
Authors: Mohsenian Ahmadreza , Khorshidi Hossein , Szymkiewicz Romuald 
Published: February 2023

Abstract
Groundwater is a vital water resource which has a significant role in the irrigation and food industry. Drawdown is a change in groundwater level due to various causes, especially pumping from wells. Forecasting water level oscillations is an important necessity for planning the integrated management of any watershed basin. In the present study, the Theis equation was applied to stochastic analysis of groundwater flow in confined aquifers, through the Karhunen–Loeve expansion (KLE) method. The quantification of the uncertainty associated with the statistical moments of hydraulic head is the aim of this research. The KLE method takes two steps; first, aquifer transmissivity (T) as an input random field is decomposed in the form of a set of orthogonal Gaussian random expressions in which eigen structures related to the covariance function of T were obtained from the Fredholm equation. Then, the hydraulic head h(x,t) was expanded with polynomial terms in which some coefficients were computed from the governing equation. The statistical moments (i.e., mean values and variances) of h(x,t) were calculated and compared with Monte Carlo simulations (MCS) to validate the results.
Carcinoma-associated fibroblast-derived lysyl oxidase-rich extracellular vesicles mediate collagen crosslinking and promote epithelial-mesenchymal transition via p-FAK/p-paxillin/YAP signaling
Authors: Liu Tingjiao , Liu Xue , Li Jiao , Yang Xuesong , Li Xiaojie , Kong Jing , Qi Dongyuan , Zhang Fuyin , Sun Bo , Liu Yue-Hua 
Published: February 2023

Abstract
Carcinoma-associated fibroblasts (CAFs) are the main cellular components of the tumor microenvironment and promote cancer progression by modifying the extracellular matrix (ECM). The tumor-associated ECM is characterized by collagen crosslinking catalyzed by lysyl oxidase (LOX). Small extracellular vesicles (sEVs) mediate cell-cell communication. However, the interactions between sEVs and the ECM remain unclear. Here, we demonstrate, for the first time, that sEVs released from oral squamous cell carcinoma (OSCC)-derived CAFs induce collagen crosslinking, thereby promoting epithelial-mesenchymal transition (EMT). CAF sEVs preferably bound to the ECM rather than being taken up by fibroblasts and induced collagen crosslinking, and a LOX inhibitor or blocking antibody suppressed this effect. Active LOX (αLOX), but not the LOX precursor, was enriched in CAF sEVs and interacted with periostin, fibronectin, and bone morphogenetic protein-1 on the surface of sEVs. CAF sEV-associated integrin α2β1 mediated the binding of CAF sEVs to collagen I, and blocking integrin α2β1 inhibited collagen crosslinking by interfering with CAF sEV binding to collagen I. CAF sEV-induced collagen crosslinking promoted the EMT of OSCC through p-FAK/p-paxillin/YAP signaling in vitro and in vivo. Taken together, these findings reveal a novel role of CAF sEVs in tumor ECM remodeling, suggesting a critical mechanism for CAF-induced EMT of cancer cells.
Combination therapy with platelet-rich plasma and epidermal neural crest stem cells increases treatment efficacy in vascular dementia
Authors: Akbari Somayeh , Haghani Masoud , Ghobadi Mojtaba , Hooshmandi Etrat , Haghighi Afshin Borhani , Salehi Mohammad Saied , Pandamooz Sareh , Azarpira Negar , Afshari Afsoon , Zabihi Shahrbanoo , Nemati Marzieh , Bayat Mahnaz 
Published: February 2023

Abstract
This study aimed to evaluate effects of platelet-rich plasma (PRP) and neural crest-derived epidermal stem cells (ESC) in alone administration and their combination in vascular dementia (VaD) model by tow-vessels occlusion (2VO). Methods the rats were divided into control, sham, 2VO + vehicle, 2VO + PRP, 2VO + ESC, and 2VO + ESC + PRP groups. The treated-groups received one million cells on days 4, 14, and 21 with or without 500µl PRP (twice a week). The behavioral performance was evaluated by open-field, passive-avoidance, and Morris-water maze. The basal-synaptic transmission (BST) and long-term potentiation (LTP) were assessed by field-potential recording. The mRNA expression levels of IGF-1, TGF-β1, PSD-95, and GSk-3β were measured in rat hippocampus by qRT-PCR. Results The results showed a learning, memory, and synaptic plasticity impairment with a significant decrease in IGF1, TGF-β1, PSD-95, and increase in expression of GSk-3β 2VO rats. Although treatment with ESC alone and ESC + PRP show comparable improvement effects on spatial memory and LTP induction associated with PSD-95 up-regulation and GSK-3β down-regulation, the BST only recovered in the ESC + PRP group. Moreover, combination therapy was superior to monotherapy with PRP. Conclusions The transplantation of ESC showed better effects than PRP alone, and combination therapy increased treatment efficacy with the recovery of BST.
Effect of the transjugular intrahepatic portosystemic shunt on peptidomics composition of peripheral plasma
Authors: Bagarolo Giulia Ilaria , Schierwagen Robert , Gu Wenyi , Jankowski Vera , Soppert Josefin , Barzakova Emona , Cascone Federica , Tyc Olaf , Kuhl Christiane , Noels Heidi , Trebicka Jonel , Jankowski Joachim 
Published: February 2023

Abstract
Background & Aims
Portal hypertension often develops in patients with advanced chronic liver diseases (CLD), especially cirrhosis and is associated with complications of the disease, such as gastrointestinal bleeding and ascites, which account for high mortality rates. The transjugular intrahepatic portosystemic shunt (TIPS) is one of only a few treatment options for portal hypertension aiming at the decrease of portal venous pressure by establishing an artificial passage for blood from the gastrointestinal tract directly to the liver vein. The current study aimed to investigate the molecular composition of plasma samples from patients with portal hypertension to characterize mediators influenced by TIPS intervention and involved in gut-liver crosstalk.
Methods
The plasma of 23 patients suffering from advanced CLD with portal hypertension was collected from peripheral veins before and after TIPS treatment and was analyzed by liquid chromatography-mass spectrometry (LC-MS) using a non-targeted approach. Substances in the molecular range of 100 to 1,500 (m/z) with concentration differences induced by the TIPS intervention were identified in peripheral blood. The identified mediators were sequenced through MS/MS and analyzed through literature mining to gain an insight into their function.
Results
Overall, eleven low molecular weight mediators revealed concentration changes triggered by the TIPS intervention. From these, four peptides and six metabolites were significantly decreased after TIPS, whereas one metabolite showed an increase after the medical procedure.
Conclusions
In conclusion, we identified in this study ten new biomarker candidates for portal hypertension in patients with CLD, with potential involvement in the regulation of pathological gut-liver crosstalk.
Evaluating Occupant Feedback on Indoor Air Quality Perception During Covid Stay-at-home Using Social Media Data: A Nationwide Study in the U.S.
Authors: Ashayeri Mehdi , Piri Soroush , Abbasabadi Narjes 
Published: February 2023

Abstract
Despite challenges, the COVID stay-at-home has provided opportunities to reveal unforeseen complexities within built environments, particularly from indoor health lenses, that had not been sought before the pandemic. This research aimed to evaluate occupants’ feedback on impacts of the stay-at-home on the indoor air quality (IAQ) perception in buildings nationwide in the U.S. during the first year of the pandemic (2019) and compare it with the baseline (2019). We used geo-tagged big textual data obtained from Twitter platform and developed Natural Language Processing (NLP) models based on Sentiment Analysis (SA) approach to compute the occupant feedback for the two consecutive years. We built the SA models through developing a six-step workflow, including data acquisition; data cleaning; text tokenization; analysis; accuracy evaluation; and data visualization and mapping. We used the QDAP dictionary and nrc lexicon to develop the SA models. We also developed automation scripts to improve the simulation performance. Results illustrate that occupants’ complaints on IAQ increased during 2020 compared with the baseline (2019). Findings further suggest that occupants with less access to operative Heating, Cooling, and Air Conditioning (HVAC) systems posted more dissatisfied feelings on Twitter. This research aids decision-makers to better understand the impacts of lockdown on occupants’ health experience to rethink the design and operation of buildings in a way to promote human-centered design and improving building resilience against future pandemics.
A long-read sequencing and SNP haplotype-based novel preimplantation genetic testing method for female ADPKD patient with de novo PKD1 mutation
Authors: Peng Cuiting , Chen Han , Ren Jun , Zhou Fan , Li Yutong , Keqie Yuezhi , Ding Taoli , Ruan Jiangxing , Wang He , Chen Xinlian , Liu Shanling 
Published: February 2023

Abstract
The autosomal dominant form of polycystic kidney disease (ADPKD) is the most common hereditary disease that causes late-onset renal cyst development and end-stage renal disease. Preimplantation genetic testing for monogenic disease (PGT-M) has emerged as an effective strategy to prevent pathogenic mutation transmission rely on SNP linkage analysis between pedigree members. Yet, it remains challenging to establish reliable PGT-M methods for ADPKD cases or other monogenic diseases with de novo mutations or without a family history. Here we reported the application of long-read sequencing for direct haplotyping in a female patient with de novo PKD1 c.11526 G > C mutation and successfully established the high-risk haplotype. Together with targeted short-read sequencing of SNPs for the couple and embryos, the carrier status for embryos was identified. A healthy baby was born without the PKD1 pathogenic mutation. Our PGT-M strategy based on long-read sequencing for direct haplotyping combined with targeted SNP haplotype can be widely applied to other monogenic disease carriers with de novo mutation.
The influence of maternal factors on the neonatal microbiome and health
Authors: Zhu Bin , Serrano Myrna , Buck Gregory 
Published: February 2023

Abstract
The human microbiome plays an essential role in human health. However, the influence of maternal factors on the neonatal microbiome remains obscure. Herein, our observations suggest that the neonatal buccal microbiome is similar to the maternal buccal microbiome, but the neonatal gastrointestinal microbiome develops a unique composition at an early stage. The low complexity of the neonatal buccal microbiome is a hallmark of maternal and neonatal health, but that of the neonatal gastrointestinal microbiome is associated with maternal inflammation-related metabolites. Microbial infections in the maternal reproductive tract universally impact the complexity of the neonatal microbiomes, and the body site is most important in modulating the composition of the neonatal microbiomes. Additionally, maternal lipids attenuated the adverse influence of several maternal factors on the neonatal microbiomes. Finally, admission of neonates to the newborn intensive care unit is associated with sub-optimal states of the maternal buccal and rectal microbiomes and maternal health.
Bcl-2 family inhibitors sensitize human cancer models to target therapy
Authors: Valentini Elisabetta , Martile Marta Di , Brignone Matteo , Caprio Marica Di , Manni Isabella , Chiappa Michela , Sergio Ilaria , Chiacchiarini Martina , Bazzichetto Chiara , Conciatori Fabiana , D'Aguanno Simona , D'Angelo Carmen , Ragno Rino , Russillo Michelangelo , Colotti Gianni , Felli Maria , Damia Giovanna , Bufalo Donatella Del 
Published: February 2023

AbstractBH3 mimetics, targeting Bcl-2 family anti-apoptotic proteins, represent a promising therapeutic opportunity in tumors. ABT-199, the first specific Bcl-2 inhibitor, has been approved by FDA for treating several hematological malignancies. We have recently discovered IS21, a novel pan BH3 mimetic with preclinical anti-tumor activity in different cancers. This study aimed to evaluate the efficacy of different BH3 mimetics both as single agents, in a panel of different tumor cell histotypes, and in combination with the currently used target therapy in ovarian cancer and melanoma. Our results demonstrate that IS21 reduced the viability of T-cell acute lymphoblastic leukemia, melanoma, lung, pancreatic and ovarian cancer cell lines, and that Bcl-xL and Mcl-1 protein levels were markers of IS21 sensitivity in melanoma and ovarian cancer, respectively. Exploring the IS21 mechanism of action, we reported that IS21 activity was dependent on BAX and BAK proteins, and complexes between Bcl-2 and Bcl-xL proteins and their main binding partners were reduced by IS21. In combination experiments, BH3 mimetics sensitized ovarian cancer cells to the treatment with PARP inhibitors, while IS21 and ABT-199 synergized with MAPK inhibitors in melanoma models bothin vitroandin vivo.Through different methodological approaches, we evidenced that the potentiating effect of BH3 mimetics was related to enhancement of apoptotic pathway, both in melanoma and ovarian cancer. In conclusion, our data suggest the use of inhibitors of the anti-apoptotic proteins as a possible therapeutic strategy to enhance the efficacy of target therapy in ovarian cancer and melanoma.
Recruitment and retention challenges and strategies in randomized controlled trials of psychosocial interventions for children with cancer and their parents: a collective case study
Authors: Driessche Anne van , Beernaert Kim , Deliens Luc , Kars Marijke , Lyon Maureen , Barrera Maru , Dussel Veronica , Bidstrup Pernille , Rosenberg Abby R. , Akard Terrah F. , Cohen Joachim , De Vleminck Aline 
Published: February 2023

Abstract
Objective

In pediatric oncology there are few examples of successful recruitment and retention strategies in psychosocial care research. This study aims to summarize experiences, challenges, and strategies for conducting randomized controlled trials (RCTs) from psychosocial intervention studies among children with cancer and their parent(s).

Methods

We conducted a collective case study. To identify the cases, Pubmed and two trial registries were searched for ongoing and finished RCTs of psychosocial intervention studies for children with cancer and their parents. Online semi-structured expert interviews discussing recruitment and retention challenges and strategies were performed with principal investigators and research staff members of the intervention studies.

Results

Nine studies were identified. Investigators and staff from seven studies participated, highlighting challenges and strategies within three major themes: eligibility, enrollment and retention. Regarding eligibility, collaborating constructively with healthcare professionals and involving them before the start of the study were essential. Being flexible, training the research staff, enabling alignment with the participants’ situation, and providing consistency in contact between the research staff member and the families were important strategies for optimizing enrollment and retention. All studies followed a stepped process in recruitment.

Conclusion

Although recruitment and retention in some selected studies were successful, there is a paucity of evidence on experienced recruitment and retention challenges in pediatric psychosocial research and best practices on optimizing them. The strategies outlined in this study can help researchers optimize their protocol and trial-implementation, and contribute to better psychosocial care for children with cancer and their parents.
Trial registration: this study is not a clinical trial.
The community pharmacist as an independent prescriber: a scoping review
Authors: Piraux Arthur , Bonnan Dylan , RAMOND-ROQUIN Aline , Faure Sébastien 
Published: February 2023

Abstract
Background
Due to the shortage of professionals, the ever-increasing needs and demands for appointments, consultation of physicians is getting harder, and delays are getting longer. To limit this issue, several countries have experimented different models of independent prescribing through pharmacies.
Aim
This study aimed to analyze the contribution of independent prescribing by community pharmacists, in primary care, using the micro, meso, macro level framework.
Method
Three different databases were used: Medline, Scopus and Embase. The search was executed on March 23rd, 2022, for each of them. Some relevant articles were added manually to the search. To be selected for the review, articles needed to be published after 2000, written in English or French, focus on independent prescribing by community pharmacists, and concern a minor condition. Original research was accepted. Articles were imported into the Covidence systematic review software to perform the review.
Results
The search resulted in 1768 articles and 846 after removing duplicates. After title and abstract screening, 792 articles were qualified as irrelevant, and 54 full text studies were reviewed for eligibility. Finally, eight studies were included in the scoping review. The various studies included are recent (from 2013 to 2021) and mostly of Canadian origin (6/8). For five of them, a quantitative study design is observed. As an independent prescriber, the community pharmacist can improve patient access to primary care, reducing consultation delays for minor conditions. In addition, patient safety is maintained, and the patient’s quality of life is increased. The pharmacist independent prescriber model seems to be cost-effective for society.
Conclusion
This review highlights the potential value and relevance of the pharmacist independent prescriber. The benefits to society are felt at all levels: micro, meso, and macro. Effectiveness and efficacy seem established, but further studies are needed in other fields, as efficiency.
The recombinant receptor-binding domain of a pangolin coronavirus spike by prokaryotic expression can partially induce neutralizing antibodies
Authors: Liu Shuiqing , Chen Chen , Lu Shanshan , Cheng Dongxiao , Luo Shengdong , Fan Huahao , Chen Zeliang , Tong Yigang , Song Lihua 
Published: February 2023

Abstract
We previously reported that a SARS-CoV-2 related pangolin coronavirus GX_P2V can induce neutralizing antibodies against SARS-CoV-2 in a golden hamster model. The two viruses GX_P2V and SARS-CoV-2 have genomes with high homology and both use ACE2 as their receptor, but have distinct receptor-binding domains (RBDs) of their spike proteins. The shared neutralizing epitopes of these two viruses are unknown. Here we describe a novel method of soluble expression of GX_P2V RBD in E. coli by tagging RBD with a carboxy-terminal fragment of the SADS coronavirus nucleocapsid protein. The recombinant GX_P2V RBD have human ACE2-binding activities. Mice immunized with this recombinant RBD produced significant titers of neutralizing antibodies against GX_P2V pseudoviruses, not SARS-CoV-2 pseudoviruses. Our data suggest that the GX_P2V RBD has none or limited neutralizing epitopes against SARS-CoV-2, thus neutralizing antibodies against SARS-CoV-2 from GX_P2V infected golden hamsters is likely RBD-independent.
The effects of antenatal corticosteroid and sex on morbidity and mortality in preterm infants: a cohort study
Authors: Şeker Erdal , Kraja Elvis , Çakır Maide Selin , Kostekci Yasemin Ezgi , Okulu Emel , Erdeve Ömer , Atasay Fatma Begüm , Arsan Saadet , Koç Acar 
Published: February 2023

Abstract
Objective: To study the linked result of a complete course of antenatal corticosteroids (ANS) on mortality and short-term morbidity rates among preterm infants in our population.
Study design: This single-center retrospective study included the infants born before 32 weeks’ gestation and admitted to neonatal intensive care unit (NICU) between January 1, 2018 and December 31, 2020. The following data of gestational age, birth weight, sex, the etiology of labor, type of delivery, need for intubation in delivery room, APGAR scores (1st and 5th min), the rates of respiratuvary distress syndrome (RDS), surfactant administration, patent ductus arteriosus (PDA), retinopathy of prematurity (ROP), necrotizing enterocolitis (NEC), intraventricular hemorrhage (IVH), and bronchopulmonary dysplasia (BPD) were collected from medical records.
Results: The study included 210 infants with a median gestational age of 28.6 weeks (24-31.6), a birth weight of 1065 g (445-2165) and with an ANS use rate of 80%. The mortality rate was lower (p=0.001) with a longer hospital stay (p=0.029), but the rate of BPD was higher (p=0.014) in male infants who received ANS than who did not. According to sex distribution, there was a significant decrease in mortality rate in male infants compared with female ones in ANS received group (11% vs. 23%, p=0.038) with a higher BPD rate (p=0.005).
Conclusion: ANS is related with less mortality in male infants born before 32 weeks’ gestation. Further research is currently needed to evaluate advantage of antenatal steroids in different populations.