bluuebunny/crossref_metadata_2025_split huggingface dataset:
iASPP regulates neurite development by interacting with Spectrin proteins
Authors: Wang Junhao , Jia Chunhong , Gao Qiong , Zhang Jiwen , Gu Xi 
Published: May 2023

IntroductionSince its discovery in 1999, a substantial body of research has shown that iASPP is highly expressed in various kinds of tumors, interacts with p53, and promotes cancer cell survival by antagonizing the apoptotic activity of p53. However, its role in neurodevelopment is still unknown.MethodsWe studied the role of iASPP in neuronal differentiation through different neuronal differentiation cellular models, combined with immunohistochemistry, RNA interference and gene overexpression, and studied the molecular mechanism involved in the regulation of neuronal development by iASPP through coimmunoprecipitation coupled with mass spectrometry (CoIP-MS) and coimmunoprecipitation (CoIP).ResultsIn this study, we found that the expression of iASPP gradually decreased during neuronal development. iASPP silencing promotes neuronal differentiation, while its overexpression inhibited neurite differentiation in a variety of neuronal differentiation cellular models. iASPP associated with the cytoskeleton-related protein Sptan1 and dephosphorylated the serine residues in the last spectrin repeat domain of Sptan1 by recruiting PP1. The non-phosphorylated and phosphomimetic mutant form of Sptbn1 inhibited and promoted neuronal cell development respectively.ConclusionOverall, we demonstrate that iASPP suppressed neurite development by inhibiting phosphorylation of Sptbn1.
Comparation of differences in the performance of corporate social responsibility between Chinese and American pharmaceutical enterprises—based on corporate social responsibility report
Authors: Xu HongHao , Zhang Meng , Chu ShuZhen 
Published: May 2023

Objective: We compared Chinese and American pharmaceutical companies’ corporate social responsibility (CSR) reports to determine their differences and to analyze the possible reasons for them.Methods: We took as a model the top 500 pharmaceutical companies from Torreya’s (a global investment bank) list of the 1,000 most valuable pharmaceutical companies in the world. We then collected the 2020 corporate social responsibility reports of 97 Chinese and 94 American pharmaceutical companies. These reports were analyzed using software such as ROST Content Mining 6.0 and Gephi 0.92.Results: We formed a high-frequency word list, a semantic network diagram, and a high-frequency word centrality scale for the Chinese and American pharmaceutical corporate social responsibility reports. The Chinese pharmaceutical companies’ corporate social responsibility reports formed a layout of “double centers and double themes,” and the text paid more attention to the disclosure of environmental protection information. The American pharmaceutical companies formed a report presentation form of “three centers and two themes,” focusing on corporate social responsibility information disclosures from the perspective of humanistic care.Discussion: The differences in between Chinese and American pharmaceutical companies’ corporate social responsibility reports may be due to different corporate development strategies, regulatory requirements, social demands, and the concept of “corporate citizenship.” This study makes recommendations for Chinese pharmaceutical companies to better fulfill their CSR at three levels: policy-making, company management, and society.
STC3141 improves acute lung injury through neutralizing circulating histone in rat with experimentally-induced acute respiratory distress syndrome
Authors: Ge Yangyang , Wang Chenchen , Yao Chenye , Wang Yong , Zheng Yuduo , Luo Junjie , Chen Jiayi , Wang Yu , Wang Fuquan , Wang Li , Lin Yun , Shi Lin , Yao Shanglong 
Published: May 2023

Background: Acute respiratory distress syndrome (ARDS) remains a challenge because of its high morbidity and mortality. Circulation histones levels in ARDS patients were correlated to disease severity and mortality. This study examined the impact of histone neutralization in a rat model of acute lung injury (ALI) induced by a lipopolysaccharide (LPS) double-hit.Methods: Sixty-eight male Sprague-Dawley rats were randomized to sham (N = 8, received saline only) or LPS (N = 60). The LPS double-hit consisted of a 0.8 mg/kg intraperitoneal injection followed after 16 h by 5 mg/kg intra-tracheal nebulized LPS. The LPS group was then randomized into five groups: LPS only; LPS +5, 25, or 100 mg/kg intravenous STC3141 every 8 h (LPS + L, LPS + M, LPS + H, respectively); or LPS + intraperitoneal dexamethasone 2.5 mg/kg every 24 h for 56 h (LPS + D). The animals were observed for 72 h.Results: LPS animals developed ALI as suggested by lower oxygenation, lung edema formation, and histological changes compared to the sham animals. Compared to the LPS group, LPS + H and +D groups had significantly lower circulating histone levels and lung wet-to-dry ratio, and the LPS + D group also had lower BALF histone concentrations; the blood neutrophils and platelets counts in LPS + D group did not change, meanwhile, the LPS + L, +M and +H groups had significantly lower neutrophil counts and higher platelet counts in the blood; the total number of BALF WBC, platelet counts, MPO and H3 were significantly lower in the LPS + L, +M, +H and +D groups than in the LPS only group; and the degree of inflammation was significantly less in the LPS + L, +M, +H and +D groups, moreover, inflammation in the LPS + L, +M and +H animals showed a dose-dependent response; finally, the LPS + L, +M, +H and +D groups had improved oxygenation compared to the LPS group, and there were no statistical differences in PCO2 or pH among groups. All animals survived.Conclusion: Neutralization of histone using STC3141, especially at high dose, had similar therapeutic effects to dexamethasone in this LPS double-hit rat ALI model, with significantly decreased circulating histone concentration, improved acute lung injury and oxygenation.
Cardioprotective effect of curcumin on myocardial ischemia/reperfusion injury: a meta-analysis of preclinical animal studies
Authors: Zeng Yi-Fan , Guo Qi-Hao , Wei Xin-Yu , Chen Si-Yu , Deng Sheng , Liu Ji-Jia , Yin Ni , Liu Yan , Zeng Wen-Jing 
Published: May 2023

Objective: This meta-analysis aimed to determine the efficacy of curcumin in preventing myocardial ischemia/reperfusion (I/R) injury in animal models.Methods: Studies published from inception to January 2023 were systematically searched in databases including PubMed, Web of Science, Embase, China’s National Knowledge Infrastructure (CNKI), Wan-Fang database, and VIP database (VIP). The SYRCLE’s RoB tool was used to determine methodological quality. Sensitivity analysis and subgroup analysis were performed when there was high heterogeneity. Publication bias was assessed using a funnel plot.Results: Thirty-seven studies involving 771 animals were included in this meta-analysis with methodology quality scores ranging from 4 to 7. The results indicated that curcumin treatment significantly improved myocardial infarction size standard mean difference (SMD) = −5.65; 95% confidence interval (CI): 6.94, −4.36; p &lt; 0.01; I2 = 90%). The sensitivity analysis for infarct size showed that the results were stable and reliable. However, the funnel plot was asymmetric. The subgroup analysis included species, animal model, dose, administration, and duration. The results showed that the subgroup dose was statistically significant between subgroups. In addition, curcumin treatment improved cardiac function, myocardial injury enzymes, and oxidative stress levels in animal models of myocardial I/R injury. The funnel plot revealed that there is publication bias for creatine kinase and lactate dehydrogenase. Finally, we performed a meta-analysis of inflammatory cytokines and apoptosis index. The results showed that curcumin treatment downregulated serum inflammatory cytokine levels and myocardial apoptosis index.Conclusion: This meta-analysis suggests that curcumin has excellent potential for the treatment of myocardial I/R injury in animal models. However, this conclusion needs to be further discussed and verified in large animal models and human clinical trials.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42022383901.
Alterations in the gut microbiota and serum metabolomics of spontaneous cholestasis caused by loss of FXR signal in mice
Authors: Wei Shizhang , He Tingting , Zhao Xu , Jing Manyi , Li Haotian , Chen Lisheng , Zheng Ruimao , Zhao Yanling 
Published: May 2023

Background: Farnesoid X receptor (FXR) is a key metabolic target of bile acids (BAs) and is also a target for drugs against several liver diseases. However, the contribution of FXR in the pathogenesis of cholestasis is still not fully understood. The purpose of this study is to provide a comprehensive insight into the metabolic properties of FXR-involved cholestasis in mice.Materials and methods: In this study, an alpha-naphthylisothiocyanate (ANIT)-induced cholestasis mouse model and FXR−/− mice were established to investigate the effect of FXR on cholestasis. The effect of FXR on liver and ileal pathology was evaluated. Simultaneously, Untargeted metabolomics combined with 16s rRNA gene sequencing analysis was applied to reveal the involvement of FXR in the pathogenesis of cholestasis.Results: The results showed that ANIT (75 mg/kg) induced marked cholestasis in WT and FXR −/− mice. It is noteworthy that FXR−/− mice developed spontaneous cholestasis. Compared with WT mice, significant liver and ileal tissue damage were found. In addition, 16s rRNA gene sequencing analysis revealed gut microbiota dysbiosis in FXR−/− mice and ANIT-induced cholestasis mice. Differential biomarkers associated with the pathogenesis of cholestasis caused by FXR knockout were screened using untargeted metabolomics. Notably, Lactobacillus_ johnsonii_FI9785 has a high correlation with the differential biomarkers associated with the pathogenesis and progression of cholestasis caused by FXR knockout.Conclusion: Our results implied that the disorder of the intestinal flora caused by FXR knockout can also interfere with the metabolism. This study provides novel insights into the FXR-related mechanisms of cholestasis.
Hotspots, trends, and advice: a 10-year visualization-based analysis of painting therapy from a scientometric perspective
Authors: Liang Qianrong , Ye Jiarong , Lu Yingyin , Dong Junjie , Shen Heyong , Qiu Hongzhong 
Published: May 2023

PurposeResearch on painting therapy is available worldwide and painting therapy is widely applied as a psychological therapy in different fields with diverse clients. As an evidence-based psychotherapy, previous studies have revealed that painting therapy has favorable therapeutic effects. However, limited studies on painting therapy used universal data to assemble in-depth evidence to propose a better recommendation on it for the future use. Large-scale retrospective studies that used bibliometric methodology are lacking. Therefore, this study presented a broad view of painting therapy and provided an intensively analytical insight into the structure of knowledge regarding painting therapy employing bibliometric analysis of articles. CiteSpace software was used to evaluate scientific research on painting therapy globally published from January 2011 to July 2022.MethodsPublications related to painting therapy from 2011 to 2022 were searched using the Web of Science database. This study employed bibliometric techniques to perform co-citation analysis of authors, visualize collaborations between countries/regions as network maps, and analyze keywords and subjects relevant to painting therapy by using CiteSpace software.ResultsIn total, 871 articles met the inclusion criteria. We found that the number of painting therapy publications generally trended incrementally. The United States and United Kingdom made the most contributions to painting therapy research and had the greatest impact on the practical application in other countries. Arts in Psychotherapy and Frontiers in Psychology occupied key publishing positions in this research field. The application groups were mainly children, adolescents, and females, and Western countries paid high attention to painting therapy. The main areas of application of painting therapy were Alzheimer’s disease and other psychosomatic disease fields. Identified research priorities for painting therapy were emotion regulation and mood disorder treatment, personality disorder treatment, personal self-esteem enhancement, and medical humanistic care. Three keywords, “depression,” “women,” and “recovery,” had the strongest citation bursts, which emphasized the research trends.ConclusionThe general trend for painting therapy research is positive. Our findings provide useful information for researchers on painting therapy to determine new directions in relate to popular issues, collaborators, and research frontiers. Painting therapy holds a promising future, and further studies could explore the clinical implications of this therapy in terms of mechanisms and criteria for assessing efficacy.
The good, the bad, and the ugly: a comprehensive study of temperament and personality traits as correlates of self-reported disruptive behavior problems in male and female adolescents
Authors: Muris Peter , Bakker Ireen , Peulen Myrthe , van Mulekom Sanne , Meesters Cor 
Published: May 2023

PurposeThe aim of the present study was to provide a comprehensive picture of temperament and personality traits as correlates of self-reported disruptive behavior problems in male and female adolescents.MethodsTwo-hundred-and-sixty-three non-clinical adolescents aged 12–18 years completed a survey containing standardized scales to measure the HEXACO personality traits, impulsivity, effortful control, Dark Triad traits, and symptoms of oppositional-defiant disorder (ODD) and conduct disorder (CD).ResultsThe results showed that good traits (in particular, honesty-humility, agreeableness, and the regulative trait of effortful control) were negatively associated, while bad and ugly traits (especially impulsivity and the Dark Triad traits of psychopathy and Machiavellianism) were positively associated with symptoms of ODD and CD. In addition, regression analyses indicated that both types of disruptive behavior problems were associated with a unique set of temperament and personality correlates, and that specific correlates also differed for both genders.ConclusionsIt is concluded that research on the role of temperament and personality should adopt a broad perspective, taking good, bad, and ugly traits from various theoretical models as well as gender differences into account.
Did China's dual-credit policy promote innovation in new energy vehicle enterprises? An empirical study based on the PSM-DID method
Authors: Sun Jianguo , Tian Mingfu 
Published: May 2023

IntroductionBased on the microdata of 36 A-share new energy vehicle (NEV) enterprises from 2015 to 2021, this study empirically investigates whether the introduction of the double credit policy (DCP) promoted the innovation of NEV enterprises.MethodsThe propensity score matching difference-in-differences (PSM-DID) method was used.ResultsThe following results were found. First, the introduction of the DCP inhibits the innovation of NEV enterprises. However, as the DCP stabilizes, the inhibitory effect gradually decreases and shows a tendency to turn into a facilitating effect. Second, the DCP affects the innovation output of enterprises by influencing their research and development (R&amp;D) investment. R&amp;D investment has a mediating role in the DCP, affecting the innovation of new energy vehicle enterprises. Third, at the firm level, the inhibitory effect of the DCP is more evident in non-state-owned enterprises (non-SOE) and insignificant for state-owned enterprises (SOE), while at the regional level, the inhibitory effect of the DCP is more evident for non-eastern regions and insignificant for eastern regions.DiscussionThis study finds the inhibitory effect of the implementation of the DCP on the innovation of NEV enterprises and have potential guiding significance for the future formulation of NEV market development policies and the promotion of high-quality development of the NEV industry.
Wastewater monitoring of SARS-CoV-2 in on-grid, partially and fully off-grid Bedouin communities in Southern Israel
Authors: Yaniv Karin , Craddock Hillary A. , Mahameed Fareed , Shagan Marilou , Salah Ikram , Lakkakula Satish , Resnick Keren , Haber Corinne , Davidovitch Nadav , Moran-Gilad Jacob , Kushmaro Ariel , Lipchin Clive 
Published: May 2023

BackgroundWastewater based epidemiology (WBE) has become an important tool in SARS-CoV-2 surveillance and epidemiology. While WBE measurements generally correlate with observed case numbers in large municipal areas on sewer grids, there are few studies on its utility in communities that are off-grid (non-sewered).Methods and materialsTo explore the applicability of wastewater surveillance in our region, five Bedouin communities along the Hebron Stream in Southern Israel (Negev desert) were sampled. One point (El-Sayed) represents a community with partial connection to the sewer grid system and another point (Um Batin) represents a community with no access to the sewer grid system. The towns of Hura, Lakia, and Tel Al-Sabi/Tel Sheva were on-grid. A total of 87 samples were collected between August 2020 to January 2021 using both grab and composite sampling. RNA was extracted from the raw sewage and concentrated sewage. RT-qPCR was carried out with N1, N2, and N3 gene targets, and findings were compared to human case data from the Israeli Ministry of Health.ResultsSARS-CoV-2 was detected consistently over time in on-grid Bedouin towns (Lakia, Tel Sheva/Tel as-Sabi, and Hura) and inconsistently in smaller, off-grid communities (El-Sayed and Um Batin). The trend in maximum copy number/L appears to be driven by population size. When comparing case numbers normalized to population size, the amount of gene copies/L was inconsistently related to reported case numbers. SARS-CoV-2 was also detected from sewage-impacted environmental waters representing communities with no access to the wastewater grid. When grab sampling and composite sampling data were compared, results were generally comparable however composite sampling produced superior results.ConclusionsThe mismatch observed between detected virus and reported cases could indicate asymptomatic or “silent” community transmission, under-testing within these communities (due to factors like mistrust in government, stigma, misinformation) or a combination therein. While the exact reason for the mismatch between environmental SARS-CoV-2 signals and case numbers remains unresolved, these findings suggest that sewage surveillance, including grab sampling methodologies, can be a critical aspect of outbreak surveillance and control in areas with insufficient human testing and off-grid communities.
Use of the superficial inferior epigastric vein in breast reconstruction with a deep inferior epigastric artery perforator flap
Authors: Varnava Charalampos , Wiebringhaus Philipp , Kampshoff David , Hirsch Tobias , Kueckelhaus Maximilian 
Published: May 2023

BackgroundAutologous breast reconstruction is highly regarded in reconstructive surgery after mastectomy. DIEP flap reconstruction represents the gold standard for autologous breast reconstruction. The major advantages of DIEP flap reconstruction are its adequate volume, large vascular caliber and pedicle length. Despite reliable anatomy, there are procedures where the plastic surgeon's creativity is required, not only to shape the new breast, but also to overcome microsurgical challenges. An important tool in these cases is the superficial epigastric vein (SIEV).Methods150 DIEP flap procedures performed between 2018 and 2021 were retrospectively evaluated for SIEV use. Intraoperative and postoperative data were analyzed. Rate of anastomosis revision, total and partial flap loss, fat necrosis and donor site complications were evaluated.ResultsIn a total of 150 breast reconstructions with a DIEP flap performed in our clinic, the SIEV was used in 5 cases. The indication for using the SIEV was to improve the venous drainage of the flap or as a graft to reconstruct the main artery perforator. Among the 5 cases, no flap loss occurred.ConclusionsUse of the SIEV is an excellent method to expand the microsurgical options in breast reconstruction with DIEP flap surgery. It provides a safe and reliable procedure to improve venous outflow in cases of inadequate outflow from the deep venous system. The SIEV could also provide a very good option for fast and reliable application as an interposition device in case of arterial complications.
Knowledge, Attitude and Practices Towards Dengue Fever Among Slum Dwellers: A Case Study in Dhaka City, Bangladesh
Authors: Rahman Md. Mostafizur , Tanni Kamrun Nahar , Roy Tuly , Islam Md. Rakibul , Al Raji Rumi Md. Alim , Sadman Sakib Mohammed , Abdul Quader Masrur , Bhuiyan Nafee-Ul-Islam , Shobuj Ifta Alam , Sayara Rahman Afra , Haque Md. Iftekharul , Faruk Fariha , Tahsan Fahim , Rahman Farzana , Alam Edris , Md. Towfiqul Islam Abu Reza 
Published: May 2023

Objectives: This study intends to evaluate Dhaka city slum dwellers’ responses to Dengue fever (DF).Methods: 745 individuals participated in a KAP survey that was pre-tested. Face-to-face interviews were performed to obtain data. Python with RStudio was used for data management and analysis. The multiple regression models were applied when applicable.Results: 50% of respondents were aware of the deadly effects of DF, its common symptoms, and its infectious nature. However, many were unaware that DF could be asymptomatic, a previously infected person could have DF again, and the virus could be passed to a fetus. Individuals agreed that their families, communities, and authorities should monitor and maintain their environment to prevent Aedes mosquito breeding. However, overall 60% of the study group had inadequate preventative measures. Many participants lacked necessary practices such as taking additional measures (cleaning and covering the water storage) and monitoring potential breeding places. Education and types of media for DF information were shown to promote DF prevention practices.Conclusion: Slum dwellers lack awareness and preventative activities that put them at risk for DF. Authorities must improve dengue surveillance. The findings suggest efficient knowledge distribution, community stimulation, and ongoing monitoring of preventative efforts to reduce DF. A multidisciplinary approach is needed to alter dwellers’ behavior since DF control can be done by raising the population’s level of life. People and communities must perform competently to eliminate vector breeding sites.