bluuebunny/crossref_metadata_2025_split huggingface dataset:
BAK1 protects the receptor-like kinase BIR2 from SNIPER2a/b-mediated degradation to promote pattern-triggered immunity in Nicotiana benthamiana
Authors: Liu Fan , Zeng Mengzhu , Sun Yujing , Chen Zhiyuan , Chen Zhaodan , Wang Lei , Cui Jia-Rong , Zhang Fushuang , Lv Di , Chen Xue , Xu Yuanpeng , Duan Kai-Xuan , Wang Yan , Wang Yuanchao 
Published: June 2023

Abstract
The detection of microbial infections by plants induces the rapid formation of immune receptor complexes at the plasma membrane. However, how this process is controlled to ensure proper immune signaling remains largely unknown. Here, we found that the Nicotiana benthamiana membrane-localized leucine-rich repeat receptor-like kinase BAK1-INTERACTING RLK 2 (NbBIR2) constitutively associates with BRI1-ASSOCIATED RECEPTOR KINASE 1 (NbBAK1) in vivo and in vitro and promotes complex formation with pattern recognition receptors. In addition, NbBIR2 is targeted by 2 RING-type ubiquitin E3 ligases, SNC1-INFLUENCING PLANT E3 LIGASE REVERSE 2a (NbSNIPER2a) and NbSNIPER2b, for ubiquitination and subsequent degradation in planta. NbSNIPER2a and NbSNIPER2b interact with NbBIR2 in vivo and in vitro and are released from NbBIR2 upon treatment with different microbial patterns. Furthermore, accumulation of NbBIR2 in response to microbial patterns is tightly associated with NbBAK1 abundance in N. benthamiana. NbBAK1 acts as a modular protein that stabilizes NbBIR2 by competing with NbSNIPER2a or NbSNIPER2b for association with NbBIR2. Similar to NbBAK1, NbBIR2 positively regulates pattern-triggered immunity and resistance to bacterial and oomycete pathogens in N. benthamiana, whereas NbSNIPER2a and NbSNIPER2b have the opposite effect. Together, these results reveal a feedback regulatory mechanism employed by plants to tailor pattern-triggered immune signaling.
Network Interventions: Applying Network Science for Pragmatic Action in Public Administration and Policy
Authors: Siciliano Michael D , Whetsell Travis 
Published: June 2023

Abstract
Public management and policy scholars have engaged in extensive development of theory and empirical study of networks and collaborative systems of governance. This scholarship has focused on understanding the mechanisms of network formation and the implications of network properties on individual and collective outcomes. Despite rich descriptive work and inferential analyses, little work has attempted to intervene in these systems. In this article, we develop the foundation for a new body of research in our field focused on network interventions. Network interventions are defined as the purposeful use of network data to identify strategies for accelerating behavior change, improving performance, and producing desirable outcomes (Valente, 2012). We extend network intervention strategies from the field of public health to public sector interorganizational and governance networks. Public sector actors have an interest in network interventions based on the fundamental pursuit of efficiency, effectiveness, and equity. Network interventions can increase the uptake of an organizational change among employees, improve the performance of a governance system, or promote the spread of a successful policy across jurisdictions. We provide scholars and practitioners with a useful way to conceptualize where, why, and how network interventions might be deployed in the pursuit of public value.
Antiprotozoal activity of auranofin on Trypanosoma cruzi, Leishmania tropica and Toxoplasma gondii: in vitro and ex vivo study
Authors: Yıldırım Ahmet , Özbilgin Ahmet , Yereli Kor 
Published: June 2023

ABSTRACT

Background
Three obligate intracellular protozoan parasite species, which are responsible for significant morbidity and mortality and settle in macrophage cells, affect more than one-half of the world's population, namely, Trypanosoma cruzi, Leishmania tropica and Toxoplasma gondii, which are causative agents of Chagas disease, leishmaniasis and toxoplasmosis, respectively. In the current study, it was aimed to investigate the in vitro and ex vivo antiprotozoal activity of auranofin on T. cruzi, L. tropica and T. gondii.

Methods
The in vitro drug efficacy (IC50) of auranofin was investigated by haemocytometry and the CellTiter-Glo assay methods and the ex vivo drug efficacy (IC50) by light microscopic examination of Giemsa-stained slides. Also, the cytotoxic activity (CC50) of auranofin was examined by the CellTiter-Glo assay. The selectivity index (SI) was calculated for auranofin.

Results
According to IC50, CC50 and SI data, auranofin did not exhibit cytotoxic activity on Vero cells, but exhibited antiprotozoal activity on epimastigotes and intracellular amastigotes of T. cruzi, promastigotes and intracellular amastigotes of L. tropica and intracellular tachyzoites of T. gondii (p&lt;0.05).

Conclusions
The detection antiprotozoal activity of auranofin on T. cruzi, L. tropica and T. gondii according to the IC50, CC50 and SI values is considered an important and promising development. This is significant because auranofin may be an effective alternative treatment for Chagas disease, leishmaniasis and toxoplasmosis in the future.
Editors as Gatekeepers: One Medical Education Journal’s Efforts to Resist Racism in Scholarly Publishing
Authors: Wyatt Tasha R. , Bullock Justin L. , Andon Anabelle , Odukoya Erica J. , Torres Carlos G. , Gingell Gareth , Han Heeyoung , Zaidi Zareen , Mylona Elza , Torre Dario , Cianciolo Anna T. 
Published: June 2023

Abstract

Purpose
Journals have begun to expand the racial diversity of editors as a first step to countering institutional racism. Given the power editors hold as gatekeepers, a diverse team helps ensure that minoritized scholars have equal opportunity to contribute. In 2021, Teaching and Learning in Medicine (TLM) created an editorial internship for racially minoritized individuals. This study examines the first 6 months of this program to better understand its creation and initial successes.

Method
The authors employed critical collaborative autoethnography, a qualitative methodology, focusing on the underlying assumptions around power and hierarchy that are implicit in the design and implementation of the TLM internship. Participants included 13 TLM editorial board members (10 internship selection committee members, 3 mentors, 2 independent researchers), 3 external selection committee members, and 3 interns, with some holding multiple roles. Ten participants served as authors of this report. Data included archival emails, planning documents, and focus groups. The initial analysis explored what happened and how and was followed by a thematic analysis in which participants reflected on their responsibility for implementing an antiracist program.

Results
While the program developed interns’ editorial skills, which they greatly valued, and diversified the TLM editorial board, it did not achieve the goal of fostering antiracism. Mentors focused on conducting joint peer reviews with interns, assuming that racial experiences can and should be separate from the editorial process, thus working within, rather than trying to change, the existing racist system.

Conclusions
Given these findings, greater structural change is needed to disrupt the existing racist system. These experiences underscore the importance of recognizing the harmful impact a race-neutral lens can have on antiracist efforts. Moving forward, TLM will implement lessons learned ahead of offering the internship again with the goal of creating the transformative change intended with the creation of the program.
A Novel Fusion Partner, SP100, Drives Nuclear Dot Localization of ALK in Epithelioid Fibrous Histiocytoma
Authors: Russell-Goldman Eleanor , Dong Fei , Laga Alvaro , Hanna John 
Published: June 2023

Abstract:
Epithelioid fibrous histiocytoma (EFH) is a distinctive benign cutaneous neoplasm composed of uniform epithelioid cells, often with binucleated cells. EFH are characterized by the presence of anaplastic lymphoma kinase (ALK) gene rearrangements with a variety of binding partners. These rearrangements result in the overexpression of ALK, which can be detected using immunohistochemistry. Cytoplasmic ALK expression is by far the most common pattern encountered. Here, we describe a case of EFH with a distinctive intranuclear dot-like ALK expression pattern. Subsequent next-generation DNA sequencing revealed a novel SP100::ALK gene fusion. Speckled protein-100 (SP100) is a constituent of nuclear dots, also known as promyelocytic leukemia bodies, which are still poorly understood membraneless subnuclear structures. Thus, this novel ALK fusion partner seems to explain this distinctive pattern of ALK localization. We examined ALK expression patterns in 11 other cases of EFH, but all showed typical cytoplasmic localization. This study expands the morphologic and molecular spectrum of EFH, provides a dramatic illustration of the ability of fusion partners to control protein localization, and implies that tumorigenic ALK signaling may occur at a variety of subcellular locations.
A Rare Case of a Basomelanocytic Tumor
Authors: Wen Daniel , Chen Tony , Martin Richard C. W. 
Published: June 2023

Abstract:
Basal cell carcinomas and melanoma are common cutaneous malignancies. However, the development of a basomelanocytic tumor that simultaneously includes elements of melanoma and basal cell carcinoma is extremely rare. We present the case of an 84-year-old man who presented with a nonpigmented, nonulcerated pink nodule of his left upper back and discuss the current management recommendations for basomelanocytic tumors.
Histopathologic Spectrum of Alopecias Seen in a Jamaican Setting
Authors: Ho Jonathan D. , Collie Chico J. , Spencer Sherri-Ann 
Published: June 2023

Abstract:
Alopecia is common in Jamaican, primarily Afro-Caribbean patients. We performed a retrospective review examining the histopathologic alopecia diagnoses over ∼5 years. Requisition forms and pathology reports were assessed. Demographic/clinical/technical/diagnostic and pathologic findings of chronicity/severity data were recorded. Three hundred thirty-eight biopsies were included. The majority were 4 mm punches, grossed horizontally. The F:M ratio was 4.8:1, mean age = 42.7 years, and mean duration of alopecia = 5.1 years. Cicatricial alopecias (CAs) predominated over non-CAs (NCAs). The top 10 diagnoses were central centrifugal CA (21.9%), folliculitis decalvans (10.9%), multifactorial alopecias (10.1%), pattern hair loss (8%), lichen planopilaris (7.1%), alopecia areata (6.2%), discoid lupus erythematosus (6.2%), nonclassifiable lymphocytic scarring alopecias (5.6%), frontal fibrosing alopecia (5.3%), and nonspecific NCAs (5%). This contrasted with other richly pigmented populations where discoid lupus erythematosus predominates. Other interesting findings included relatively frequent folliculitis decalvans and lichen planus pigmentosus in 40.9% of frontal fibrosing alopecia cases. Scarring/nonscarring clinicopathologic congruence occurred in 83.4%.
Regarding histopathologic features of severity/chronicity, CAs had markedly decreased hair counts. Perifollicular fibrosis affecting retained hairs occurred in 75% of CAs, moderate to severe in >50% of these. Approximately 50% of NCA samples demonstrated advanced miniaturization (T:V ratio <2:1). In our study, relatively young women with chronic hair loss and CA are most frequently biopsied. Central centrifugal CA is the most common diagnosis. Local features of chronic/severe disease are seen microscopically. Clinical impression of scarring/nonscarring correlates well with histopathology.
Community-level social vulnerability and individual socioeconomic status on liver transplant referral outcome
Authors: Yilma Mignote , Cogan Raymond , Shui Amy M. , Neuhaus John M. , Light Carolyn , Braun Hillary , Mehta Neil , Hirose Ryutaro 
Published: June 2023

Background:
Recent endeavors emphasize the importance of understanding early barriers to liver transplantation (LT) by consistently collecting data on patient demographics, socioeconomic factors, and geographic social deprivation indices.

Methods:
In this retrospective single-center cohort study of 1657 adults referred for LT evaluation, we assessed the association between community-level vulnerability and individual socioeconomic status measures on the rate of waitlisting and transplantation. Patients’ addresses were linked to Social Vulnerability Index (SVI) at the census tract–level to characterize community-level vulnerability. Descriptive statistics were used to describe patient characteristics. Multivariable cause-specific HRs were used to assess the association between community-level vulnerability, individual measures of the socioeconomic status, and LT evaluation outcomes (waitlist and transplantation).

Results:
Among the 1657 patients referred for LT during the study period, 54% were waitlisted and 26% underwent LT. A 0.1 increase in overall SVI correlated with an 8% lower rate of waitlisting (HR 0.92, 95% CI 0.87–0.96, p < 0.001), with socioeconomic status, household characteristics, housing type and transportation, and racial and ethnic minority status domains contributing significantly to this association. Patients residing in more vulnerable communities experienced a 6% lower rate of transplantation (HR 0.94, 95% CI 0.91– 0.98, p = 0.007), with socioeconomic status and household characteristic domain of SVI significantly contributing to this association. At the individual level, both government insurance and employment status were associated with lower rates of waitlisting and transplantation. There was no association with mortality prior to waitlisting or mortality while on the waitlist.

Conclusion:
Our findings indicate that both individual and community measures of the socioeconomic status (overall SVI) are associated with LT evaluation outcomes. Furthermore, we identified individual measures of neighborhood deprivation associated with both waitlisting and transplantation.
The prevalence and risk factors for cognitive impairment in obesity and NAFLD
Authors: Wernberg Charlotte W. , Grønkjær Lea L. , Gade Jacobsen Birgitte , Indira Chandran Vineesh , Krag Aleksander , Graversen Jonas H. , Weissenborn Karin , Vilstrup Hendrik , Lauridsen Mette M. 
Published: June 2023

Background:
Severe obesity may be accompanied by cognitive dysfunction and NAFLD, but the associations remain unclear. We describe the prevalence and features of cognitive dysfunction and examine the associations between cognitive dysfunction and the presence and severity of NAFLD, and the associations between cognitive dysfunction and signs of other obesity-related comorbidities and neuronal damage.

Methods:
A cross-sectional study of patients with a body mass index of 35 kg/m2 underwent evaluation for bariatric surgery. They were screened for adiposity-related comorbidity and underwent a liver biopsy and basic cognitive testing with the Continuous Reaction Time test, the Portosystemic Encephalopathy Syndrome test, and the Stroop Test. A representative subgroup also underwent the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). The primary study outcome was “cognitive impairment,” defined as ≥2 abnormal basic cognitive tests and/or an abnormal RBANS. The Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) served as a biomarker for neuronal damage.

Results:
We included 180 patients; 72% were women, age 46 ± 12 years, 78% had NAFLD, and 30% with NASH without cirrhosis. 8% were cognitively impaired by the basic tests and 41% by RBANS results. Most impaired were executive and short-time memory functions. There were no associations between cognitive impairment and BMI, NAFLD presence or severity, or metabolic comorbidities. Male sex (OR: 3.67, 95% CI, 1.32–10.27) and using 2 or more psychoactive medications (5.24, 95% CI, 1.34–20.4) were associated with impairment. TREM2 was not associated with cognitive impairment.

Conclusions:
Nearly half of this severely obese study cohort exhibited measurable multidomain cognitive impairment. This was not dependent on NAFLD or another adiposity comorbidity.
Relationship between postoperative residual refractive error and preoperative corneal stiffness in small-incision lenticule extraction
Authors: Cao Huazheng , Jhanji Vishal , Wang Yan 
Published: June 2023

Purpose:
To explore the relationship between postoperative residual refractive error and preoperative corneal stiffness after small-incision lenticule extraction (SMILE).

Setting:
Hospital clinic.

Design:
Retrospective cohort study.

Methods:
Corneal stiffness was evaluated using the stress–strain index (SSI). Associations between postoperative spherical equivalent (SE) and corneal stiffness were determined using longitudinal regression analysis after adjustment for sex, age, preoperative SE, and other variables. The cohort was divided into halves to compare risk ratios for residual refraction in corneas with different SSI values. Low SSI values were defined as having less-stiff corneas and others as having stiffer corneas.

Results:
287 patients (287 eyes) were included. Greater undercorrection was found in less-stiff corneas across all follow-up timepoints (less-stiff corneas: 1 day: −0.36 ± 0.45 diopters [D], 1 month: −0.22 ± 0.36 D, and 3 months: −0.13 ± 0.15 D; stiffer corneas: −0.22 ± 0.37 D, −0.14 ± 0.35 D, and −0.05 ± 0.11 D, respectively). Postoperative refraction exhibited a mean 0.05 D undercorrection for every 0.1-unit decrease in the SSI after adjustment for variables. The SSI accounted for nearly 10% of the variance in refractive outcomes. Less-stiff corneas increased the risk ratio of postoperative absolute SE >0 D and ≥0.25 D by 2.242 (95% CI, 1.334-3.768) and 3.023 (95% CI, 1.466-6.233), respectively, compared with stiffer corneas.

Conclusions:
Postoperative residual refractive error was associated with preoperative corneal stiffness. Patients with less-stiff corneas had a 2- to 3-fold increased risk of residual refractive error after SMILE. Preoperative analysis of corneal stiffness can help modify nomogram algorithms of surgery and improve the predictability of refractive outcomes.
Organ-sparing treatment for T1 and T2 penile cancer: an updated literature review
Authors: Scornajenghi Carlo Maria , Asero Vincenzo , Bologna Eugenio , Basile Giuseppe , De Angelis Mario , Moschini Marco , Del Giudice Francesco 
Published: June 2023

Purpose of review
Penile cancer (PeCa) is an orphan disease due to its rare incidence in high-income countries. Traditional surgical options for clinical T1–2 disease, including partial and total penectomy, can dramatically affect patient's quality of life and mental health status. In selected patients, organ-sparing surgery (OSS) has the potential to remove the primary tumor with comparable oncologic outcomes while maintaining penile length, sexual and urinary function. In this review, we aim to discuss the indications, advantages, and outcomes of various OSSs currently available for men diagnosed with PeCa seeking an organ-preserving option.

Recent findings
Patient survival largely depends on spotting and treating lymph node metastasis at an early stage. The required surgical and radiotherapy skill sets cannot be expected to be available in all centers. Consequently, patients should be referred to high-volume centers to receive the best available treatments for PeCa.

Summary
OSS should be used for small and localized PeCa (T1-T2) as an alternative to partial penectomy to preserve patient's quality of life while maintaining sexual and urinary function and penile aesthetics. Overall, there are different techniques that can be used with different response and recurrence rates. In case of tumor recurrence, partial penectomy or radical penectomy is feasible, without impacting overall survival.
The Novel SLRP Family Member Lumican Suppresses Pancreatic Cancer Cell Growth
Authors: Gao Huijie , Liu Chao , Ren Qiang , Zhang Litao , Qin Wei , Wang Huiyun , Zhang Yuntao 
Published: June 2023

Objectives
The past studies clearly indicated that lumican was important in the context of pancreatic cancer (PC) onset and progression, but failed to clarify the underlying mechanistic basis for such activity. As such, we evaluated the functional importance of lumican in the context of pancreatic ductal adenocarcinoma (PDAC) to understand its mechanistic role in PC.

Methods
Lumican levels were evaluated in PDAC patient tissues via quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemistry approaches. The role of lumican was additionally assessed via transfecting PDAC cell lines (BxPC-3, PANC-1) with lumican knockdown or overexpression constructs and treating PDAC cell lines with exogenous recombinant human lumican.

Results
Lumican expression levels were significantly higher in pancreatic tumor tissues relative to healthy paracancerous tissues. Lumican knockdown in BxPC-3 and PANC-1 enhanced their proliferation and migration, but reduced cellular apoptosis. Alternatively, lumican overexpression and exogenous lumican exposure failed to alter the proliferative activity of these cells. Further, lumican knockdown in BxPC-3 and PANC-1 cells results in marked P53 and P21 dysregulation.

Conclusions
Lumican may suppress PDAC tumor growth by regulating P53 and P21, and the function of lumican sugar chains in the context of PC is worth studying in future studies.
Management of the Posthemangioma Nose: 5 Key Rhinoplasty Components
Authors: Chiodo Michael V. , Lisiecki Jeffrey L. , Rohrich Rod J. 
Published: June 2023

Summary:
Hemangiomas of the nasal tip are a relatively uncommon presentation of a common tumor. While optimal medical and surgical management of nasal tip infantile hemangiomas has been described and debated extensively in the literature, to our knowledge, there is no report of secondary aesthetic and functional rhinoplasty in these patients at skeletal maturity, until now. This special topic article provides an excellent example of the 5 key technical elements to revision rhinoplasty in the skeletally mature patient with a history of nasal tip infantile hemangioma.