bluuebunny/crossref_metadata_2025_split huggingface dataset:
Des-ocultando a Eros:
Authors: Loayza Cabezas Grimanesa Madeleine 
Published: July 2023

La reflexión fenomenológica explica la complejidad del devenir del flujo constante que es la poiesis escénica (productiva y expectatorial), en tanto fenómeno móvil y mutable. Como enfoque metodológico, las estructuras formales de la teoría fenomenológica propuesta por Sokolowski (2012) describen y analizan los dispositivos poéticos del montaje “Descripción de un cuadro”[1], enfocándose en la “tension erótica” que mantiene vivo el ritmo atávico del encuentro actor/espectador. De este modo, se incorporan nuevas herramientas y procedimientos que destacan la multiplicidad que articula la praxis teatral, y proponen nuevas vías de acceso al conocimiento, que considera las circunstancias específicas del sujeto epistémico, en acuerdo con la epistemología contemporánea.

[1] Obra de Heiner Müller, dirigida por Madeleine Loayza, autora del presente artículo, con temporadas desde 2014 hasta la actualidad. Ganadora de dos Premios Universidad Central del Ecuador, en categoría docente, como Proyecto de investigación, y como obra publicada (Retrato de Mujer con naranhjas, FAUCE Editorial, 2019).
Influence of feeding mustard oil cake and soybean meal in calf starter on growth and blood metabolites of crossbred dairy calves
Authors: Sarker Md. Abid Hasan , Hossain Nobin , Arefin Sumaiya , Bari Md. Sadakatul , Habib Md. Rezwanul , Khandakar Md. Mehedi Hasan , Islam Md. Zakirul , Islam Mohammad Ashiqul , Rashid Md. Harun-ur- , Siddiki Mohammad Shohel Rana 
Published: July 2023

Abstract

The study was designed to envisage the changes in growth measurements and blood metabolites of crossbred dairy calves in response to calf starters feeding with different protein sources. All calves were fed colostrum until they were three days old. Then, calves were given 2.5 L of fresh whole milk for up to 10 days and 3 L of fresh whole milk for up to 56 days. Calves were fed a farm starter (control), other dietary groups starter containing mustard oil cake (MOC), soybean meal (SM), and a mixture of MOC and SM as MOCSM protein sources. Starting on day 21, all the calves received starter feeds. On day 56, they were weaned, and the study included calves until day 70. Data were recorded on dry matter intake, growth measurements and blood nutrients profile. Calves consumed greater DM when MOC in the starter was supplied, which was 22.5% more than that of the control-fed group. Calves feeding with SM and MOCSM consumed almost similar quantities of DM at 220.87 and 224.87 g/head/day, respectively. The BWG of calves increased significantly by 5.04 and 4.06% more in the MOC-fed group than in the SM and MOCSM groups. MOC, SM, and MOCSM-fed calves had similar (p>0.05) BLG; however, the control diet had significantly lower BLG. Blood glucose levels did not vary among the calves fed the control, MOC, SM, and MOCSM diets. Significantly higher serum albumin, urea, and BUN concentrations were found in MOC diet-fed calves than in others. Calcium and phosphorus concentration varied significantly among dietary groups and was noticeably lower in the control group compared to the other dietary groups. The results revealed that calf starters endowed with MOC as a protein source and in combination with SM were found advantageous in improving body growth and development.
Metformin sensitizes hepatocellular carcinoma to sorafenib by facilitating NHE1 degradation
Authors: Tian Geng , Yu Juping , Yan Liao , Ye Fangyu , Gui Yanping , Li Jia , Yang Shihe , Zou Lin , Zhang Lulu , Zhao Li 
Published: July 2023

Abstract
Sorafenib is a first-line chemotherapy drug for advanced hepatocellular carcinoma (HCC), but the drug resistance appeared over time. Hypoxia and acidosis are common features of the microenvironment, which contribute to the drug resistance. Here, we verified that metformin could enhance the sensitivity of HCC cells to sorafenib under hypoxia. Furthermore, metformin altered the pH levels and reversed the acidification of extracellular environment. Mechanistically, metformin promoted the ubiquitination and degradation of NHE1, a sodium-hydrogen exchanger-1, which altered the pH regulation and decreased the sensitivity of HCC cells to chemotherapy. Finally, lung metastasis mice model, subcutaneous xenograft mice model, and orthotopic recurrence mice model were used to confirm the synergistic effects of metformin in vivo. In conclusion, metformin might be a promising adjuvant therapy to enhance the effectiveness of sorafenib in HCC patients.
Exploratory study on the metabolic similarity of denitrifying carbon sources
Authors: zhang jinsen , Xia Zhiheng , Wei Qi , Luo Fangzhou , Jiang Zhao , Ao Ziding , Chen Huiling , Niu Xiaoxu , Liu Guohua , Qi Lu , Wang Hongchen 
Published: July 2023

Abstract
Mixed carbon sources have been developed for denitrification to eliminate the “carbon dependency” problem of single carbon. The metabolic correlation between different carbon sources is significant as a guidance for the development of novel mixed carbon sources. In this study, to explore the metabolic similarity of denitrifying carbon sources, we selected alcohols (methanol, ethanol, glycerol) and saccharides carbon sources (glucose, sucrose and starch). Batch denitrification experiments revealed that methanol-acclimated sludge improved the denitrification rate of both methanol (14.42 mg-N/gMLVSS*h) and ethanol (9.65 mg-N/gMLVSS*h), whereas ethanol-acclimated sludge improved the denitrification rate of both methanol (7.80 mg-N/gMLVSS*h) and ethanol (22.23 mg-N/gMLVSS*h). In addition, the glucose-acclimated sludge and sucrose-acclimated sludge possibly improved the denitrification rate of glucose and sucrose, and the glycerol-acclimated sludge improved the denitrification rate of volatile fatty acids (VFAs), alcohols, and saccharides carbon sources. Functional gene analysis revealed that methanol, ethanol, and glycerol exhibited active alcohol oxidation and glyoxylate metabolism, and glycerol, glucose, and sucrose exhibited active glycolysis metabolism. This indicated that the similarity in the denitrification metabolism of these carbon sources was based on functional gene similarity, and glycerol-acclimated sludge exhibited the most diverse metabolism, which ensured its good denitrification effect with other carbon sources.
Development and validation of a clinical prediction model for concurrent pulmonary infection in convalescent patients with intracerebral hemorrhage
Authors: Xu Jixiang , Li Yan , Li Shaomin , Han Xiaoxiao , Chen Liang , Qi Yingliang , Zhou Xiaomei 
Published: July 2023

Abstract

Background Recovery from intracerebral hemorrhage is an important but underappreciated part of the prognosis of patients with intracerebral hemorrhage. Pulmonary infection (PI) is the most common complication that greatly affects the recovery process of patients with intracerebral hemorrhage. Dynamic nomograms to predict concurrent pulmonary infections in patients recovering from cerebral hemorrhage have not been reported. The aim of this study aims to identify the risk factors for pulmonary infection in convalescent patients with intracerebral hemorrhage, and to build and validate a clinical prediction model. Methods A total of 761 convalescent patients with intracerebral hemorrhage were included in this study. Pulmonary infection was determined based on the clinical manifestations and chest X-ray, and the patients were then divided into the PI group and non-PI group. Baseline and clinical data of the patients were retrospectively analyzed. First, univariate logistic regression was performed to initially screen out predictors. Then, the predictors were optimized using least absolute shrinkage and selection operation (LASSO) regression. Finally, multivariate logistic regression analysis was carried out on the optimized predictors to identify independent risk factors and construct a nomogram prediction model. The area under the curve (AUC), calibration curves, and decision curve analysis (DCA) were used to evaluate the discrimination, calibration, and clinical utility of the model. Results Age, antibiotic use, disturbance of consciousness, tracheotomy, dysphagia, length of bed rest, nasal feeding, and procalcitonin were associated with pulmonary infection in convalescent patients with intracerebral hemorrhage. The consistency index (C-index) of the prediction model was 0.901 (95%CI: 0.878 ~ 0.924). Repeated sampling by Bootstrap for 1000 times yielded a C-index of 0.900 (95%CI: 0.877 ~ 0.923), indicating that the model has excellent discrimination. Moreover, the Hosmer-Lemeshow test revealed a good goodness of fit of the model (P = 0.982). The DCA decision curve showed that the nomogram in PI group has a good net clinical benefit. Conclusion This study constructed a nomogram prediction model based on the demographic and clinical characteristics of convalescent patients with intracerebral hemorrhage. Further studies showed that this model is of great value in the prediction of pulmonary infection in convalescent patients with intracerebral hemorrhage.
Whole-Genome Sequencing and Analysis of Chryseobacterium arthrosphaerae from Rana nigromaculata
Authors: ZHU Lihong , LIU Hao , LI Xiaohui , SHI Yuefeng , YIN Xiaoliang , PI Xionge 
Published: July 2023

Abstract
Chryseobacterium arthrosphaerae strain FS91703 was isolated from Rana nigromaculata in our previous study. To investigate the genomic characteristics, pathogenicity-related genes, antimicrobial resistance, and genetic evolutionary relationships of this strain, PacBio RS II and Illumina HiSeq 2000 platforms were used for the whole genome sequencing. The genome size of strain FS91703 was 5,435,691 bp and GC content was 37.78%. A total of 4,951 coding genes were predicted; 52 virulence factors and 94 virulence genes were identified, with the most relevant genes involved in the virulence factor of immune regulation. Analysis of antibiotic resistance genes revealed that strain FS91703 harbored 10 antibiotic resistance genes in 6 categories and 2 multidrug-resistant efflux pump genes, including adeG and farA. Strain FS91703 was sensitive to β-lactam combination drugs, cephem, monobactam and carbapenems, intermediately resistant to phenicol, and resistant to penicillin, aminoglycosides, tetracycline, fluoroquinolones, and folate pathway inhibitors. Phylogenetic and ANI analyses suggested that strain FS91703 had the closest genetic relationship to the C. arthrosphaerae species; 16S rRNA sequencing revealed that both species were on the same branch of the evolutionary tree; FS91703 and FDAARGOS 519 (C. arthrosphaerae) or ED882-96 (C. arthrosphaerae) had ANI values of 96.69% or 96.13%, respectively. Thus, C. arthrosphaerae (FS91703) is a multidrug-resistant and highly virulent bacterium, providing theoretical support for the etiology and disease control due to C. arthrosphaerae in Rana nigromaculata. The results of this study are presented in the following pages.
What is the impact of plasminogen activator inhibitor type 1 (PAI-1) gene polymorphism on gynecological malignancies: a retrospective study
Authors: Li Xin , Yan Zhenzhen , Luo Suiyu 
Published: July 2023

Abstract

Background:Plasminogen activator inhibitor type 1 (PAI-1) is a protein that inhibits the activation of plasminogen. Some studies investigated the associations between PAI-1 4G/5G polymorphism and various cancers, but the results were inconsistent. So we aimed to observe the distribution of PAI-1 4G/5G genotypes in the patients of gynecological malignancies and gynecological benign tumors. To analyze the relationship between PAI-1 gene polymorphism and 4G/5G alleles with gynecological malignancies.Methods:Retrospectively analyzed 490 patients who underwent selective surgery from March 2022 to February 2023, in the obstetrics and gynecology department of Henan University People's Hospital. Patients subsequently were assigned into two groups, according to their postoperative pathologies. The malignancy group was 245 patients whose postoperative pathologies were gynecological malignant tumors and 245 patients whose postoperative pathological diagnosis was gynecological benign tumors were regarded as control group. We collected PAI-1 4G/5G genotypes in both groups of patients, compared baseline data and PAI-1 4G/5G genotypes between patients in the malignancy group and the control group. We analyzed the association between PAI-1 4G/5G genotypes and gynecological malignant tumors. All data analysis was performed using SPSS 25.0, variables were compared by the Wilcoxon rank sum test andχ2test.Results:The distribution of PAI-1 4G/5G polymorphism was quite different between patients in the malignancy group and the control group (P＜0.001), and 4G allelic frequency was quite different in the patients with malignancies and benign tumors (P＜0.001), and 4G allelic frequency was significantly higher in the patients with malignancies than in the control group.Conclusion:Our data suggested that there was a correlation between PAI-1 4G/5G polymorphism and gynecological malignancies, and the frequency of the PAI-1 4G/4G genotype and 4G allele was significantly higher in the patients with malignancies than in the control group.
Evaluation of the anthraquinone drug rhein at IC50 on T-47D breast cancer cell lines based on analytical methods
Authors: Heidari Pedram , Shekardasht Mehrnaz Babaei , Alipour Arash , Mehraei Negin 
Published: July 2023

Abstract

Rhubarb (Rheum ribes L.) is a perennial herb belonging to the Rheum (Polygonaceae) genus. This study aimed to isolate, purify, and characterize rhein anthraquinones from rhizomes of rhubarb and investigate their cytotoxic effects on the T-47D cell line. Separation and purification were performed by column chromatography. The identity of rhein was established by TLC, TLC densitometry, HPLC, 1HNMR, and FT-IR. In vitro, cytotoxicity and IC50 levels were assessed using the MTT assay. Various concentrations of rhein were exposed to determine the cytotoxic effect by measuring the cell proliferation activity of the T-47D breast cancer cell line after 24 and 48 h of incubation. The IC50 values determined for the Rhine were 226.53 and 229.00 ppm at 24 and 48 hours. Anthraquinones extracted from Rhein have significant anticancer activity against the T-47D cell line and may be of great therapeutic value for further study as antitumor agents.
GALECTIN-1 is involved in sperm-egg fusion via interaction with JUNO in rats
Authors: Lai Xiong , Liu Yuqi , Fan Yaochun , Li Hongxia , Han Guotao , Su Huimin , Xing Wanjin 
Published: July 2023

Abstract
Background: Sexual reproduction is the basic way for higher eukaryotes to reproduce. Fusion of sperm with oocyte is the key process for success of sexual reproduction. Multiple proteins have been identified to be implicated in mammalian sperm-egg fusion, in which only a few, such as IZUMO1 of sperm and JUNO of egg, have been verified by knockout mice to be indispensable to gamete fusion. However, interaction between IZUMO1 and its receptor JUNO mediates sperm-egg recognition and binding, but is unqualified for membrane fusion. Thus, there might be other accessorial proteins interacting with IZUMO1 or/and JUNO for the sperm-egg binding and succeeding membrane fusion.
Results: Here, we reported that treatment of rat sperm and egg with anti-Galectin-1 antibody significantly inhibits IVF rate. Galectin-1 was detected on the head and the front half flagellum of rat sperms and in cytoplasm of spermatocytes and the Sertoli cells. It was also found on cell membrane of rat MII eggs as well as in cytoplasm of cumulus cells. Galectin-1 interacts with JUNO and co-localizes with JUNO on the cell membrane and in cytoplasm of rat MII eggs.
Conclusions: Our results suggested that Galectin-1 be involved in the process of sperm-egg fusion through interaction with JUNO.
The Diversity of the Microbiome Impacts Chronic Lymphocytic Leukemia Development in Mice and Humans
Authors: Niemann Carsten , Faitova Tereza , Coelho Mariana , Cunha-Bang Caspar da , Öztürk Selcen , Kartal Ece , Bork Peer , Seiffert Martina 
Published: July 2023

Abstract
The gut microbiota play a critical role in maintaining a healthy human body and their dysregulation is associated with various diseases. In this study, we investigated the influence of the gut microbiome diversity on chronic lymphocytic leukemia (CLL) development. In the Eµ-TCL1 mouse model of CLL, we observed a faster course of disease when mice were housed in high hygiene conditions. Shotgun DNA sequencing of fecal samples showed that this was associated with a lower microbiome complexity which was dominated by Mucispirillum and Parabacteroidesgenera in comparison to mice kept under lower hygiene conditions. Stool sample analysis of CLL patients revealed individual and heterogeneous microbiome compositions, but allowed for grouping of patients according to their microbiome complexity. Interestingly, CLL patients with a lower microbiome diversity and an enrichment of bacteria linked to poor health suffered from a more progressed or aggressive form of CLL. In conclusion, we applied taxonomic microbiome analyses to demonstrate a link between the gut microbiota diversity and CLL development in mice and humans. Our novel data serve as a basis for further investigations to decipher the pathological and mechanistic role of intestinal microbiota in CLL development.
Gender differences in the observation of gesture distance: a physiological study
Authors: Gallo Fabrizia , González-Villar Alberto , Ott Laurent , Sampaio Adriana , Nandrino Jean-Louis , Bartolo Angela 
Published: July 2023

Abstract

Intransitive gestures are expressive and symbolic, whereas pantomimes are object-related actions. These gestures convey different meanings depending on whether they are directed toward (TB) or away from the body (AB). TB gestures express mental states (intransitive) or hygiene/nutritional activities (pantomime), while AB gestures modify the behavior of the observer (intransitive) or demonstrate tool use with an object (pantomime). A substantial body of literature suggests that females exhibit stronger social cue processing compared to males. Considering the social significance of gestures, this study aims to explore the physiological gender differences in the observation of AB and TB gestures.
Pupil dilation and High Frequency Heart Rate Variability (HF-HRV) were measured in 54 participants (27 female) while observing TB and AB gestures. The Interpersonal Reactivity Index (IRI) and the Vicarious Distress Questionnaire (VDQ) were used to assess social-emotional processes. Results showed greater pupil dilation in females for TB gestures, but no significant gender differences for HF-HRV. Males showed a significant correlation between increased pupil dilation to both TB and AB gestures and empathy levels (IRI). The support scale of the VDQ correlated significantly with TB gestures in males. These findings provide insight into the neurobiological basis of gender differences in perceiving social gestures.
High dimensional mass cytometry analysis unravels distinct profiles of peripheral blood mononuclear cells in patients with neuromyelitis optica spectrum disorder and myelin oligodendrocyte glycoprotein associated disease or in health
Authors: yao mengyuan , Wang Wenjing , Sun Jiali , Guo Tianshu , Bian Jiangping , Xiao Fuyao , Li Yuanyuan , Cong Hengri , Wei Yuzhen , Zhang Xinghu , Liu Jianghong , Yin Linlin 
Published: July 2023

Abstract
Background
Neuromyelitis optica spectrum disorders (NMOSD) and myelin oligodendrocyte glycoprotein-antibody associated disease (MOGAD) are rare demyelinating diseases of the central nervous system but can cause severe disability. Although these two diseases share inherent similarities, they show different pathogenesis, clinical features, and treatment response. Investigation performed by a more powerful approach is needed.
Methods
Cytometry by time-of-flight mass spectrometry (CyTOF) was used to cluster and phenotype the immune cell subsets in peripheral blood mononuclear cells (PBMCs) isolated from patients with NMOSD or MOGAD and healthy controls (HC). RNA sequencing was used to screen pivotal genes. The obtained algorithm-based data were further verified through traditional flow cytometry and qPCR analysis.
Results
We identified 29 cell clusters and found several immune cluster changes between NMOSD and MOGAD. Interestingly, no significant differences were found in the B cells fraction between patients and HC group. Immunity dysfunction was mainly attributed to changes of diverse subsets in T cells and mononuclear phagocytes (MNPs). Similar properties of two distinct CD56 + natural killer (NK) cell phenotypes and transcription factor T-bet + or chemokine receptor CCR2 + subsets were shown between patients and health.
Conclusions
Our results show an overview of the circulating PBMCs landscape of NMOSD and MOGAD patients compared to that of HC. Our data reveal that different immune phenotypes may involve in the distinct pathogenesis of NMOSD and MOGAD and highlight T cells or MNPs as a potential target for precision treatment.
Artificial intelligence-based assistance in clinical 123I-FP-CIT SPECT scan classification
Authors: wolfswinkel evander , wielaard jette , lavalaye jules , hoff jorrit , booij jan , de wit tim , habraken jan 
Published: July 2023

Abstract
Purpose
Dopamine transporter (DAT) imaging with 123I-FP-CIT SPECT is used to support the diagnosis of Parkinson’s disease (PD) in clinically uncertain cases. Previous studies showed that automatic classification of 123IFPCIT SPECT images (marketed as DaTSCAN) is feasible by using machine learning algorithms. However, these studies lacked sizable use of data from routine clinical practice. This study aims to contribute to the discussion whether artificial intelligence (AI) can be applied in clinical practice. Moreover, we investigated the need for hospital specific training data.
Methods
A convolutional neural network (CNN) named DaTNet-3 was designed and trained to classify DaTSCAN images as either normal or supportive of a dopaminergic deficit. Both a multi-site data set (n = 2412) from the Parkinson’s Progression Marker Initiative (PPMI) and an in-house data set containing clinical images (n = 932) obtained in routine practice at the St Antonius hospital (STA) were used for training and testing. STA images were labeled based on interpretation by nuclear medicine physicians. To investigate whether indeterminate scans effects classification accuracy, a threshold was applied on the output probability.
Results
DaTNet-3 trained with STA data reached an accuracy of 89.0% in correctly identifying images of the clinical STA test set as either normal or with decreased striatal DAT binding (98.5% on the PPMI test set). When thresholded, accuracy increased to 95.7%. This increase was not observed when trained with PPMI data, indicating the incorrect images were confidently classified as the incorrect class.
Conclusion
Based on results of DaTNet-3 we conclude that automatic interpretation of DaTSCAN images with AI is feasible and robust. Further, we conclude DaTNet-3 performs slightly better when it is trained with hospital specific data. This difference increased when output probability was thresholded. Therefore we conclude that the usability of a data set increases if it contains indeterminate images.