bluuebunny/crossref_metadata_2025_split huggingface dataset:
A hyperaldosteronism subtypes predictive model using ensemble learning
Authors: Karashima Shigehiro , Kawakami Masaki , Nambo Hidetaka , Kometani Mitsuhiro , Kurihara Isao , Ichijo Takamasa , Katabami Takuyuki , Tsuiki Mika , Wada Norio , Oki Kenji , Ogawa Yoshihiro , Okamoto Ryuji , Tamura Kouichi , Inagaki Nobuya , Yoshimoto Takanobu , Kobayashi Hiroki , Kakutani Miki , Fujita Megumi , Izawa Shoichiro , Suwa Tetsuya , Kamemura Kohei , Yamada Masanobu , Tanabe Akiyo , Naruse Mitsuhide , Yoneda Takashi ,   JPAS/JRAS Study Group, Karashima Shigehiro , Kometani Mitsuhiro , Kurihara Isao , Ichijo Takamasa , Katabami Takuyuki , Tsuiki Mika , Wada Norio , Oki Kenji , Ogawa Yoshihiro , Okamoto Ryuji , Tamura Kouichi , Inagaki Nobuya , Yoshimoto Takanobu , Kobayashi Hiroki , Kakutani Miki , Fujita Megumi , Izawa Shoichiro , Suwa Tetsuya , Kamemura Kohei , Yamada Masanobu , Tanabe Akiyo , Naruse Mitsuhide , Yoneda Takashi , Ito Hiroshi , Takeda Yoshiyu , Rakugi Hiromi , Yamamoto Koichi , Soma Masayoshi , Yanase Toshihiko , Fukuda Hisashi , Hashimoto Shigeatsu , Ohno Yuichi , Takahashi Katsutoshi , Shibata Hirotaka , Fujii Yuichi , Suzuki Tomoko , Ogo Atsushi , Sakamoto Ryuichi , Kai Tatsuya , Fukuoka Tomikazu , Miyauchi Shozo 
Published: February 2023

AbstractThis study aimed to develop a machine-learning algorithm to diagnose aldosterone-producing adenoma (APA) for predicting APA probabilities. A retrospective cross-sectional analysis of the Japan Rare/Intractable Adrenal Diseases Study dataset was performed using the nationwide PA registry in Japan comprised of 41 centers. Patients treated between January 2006 and December 2019 were included. Forty-six features at screening and 13 features at confirmatory test were used for model development to calculate APA probability. Seven machine-learning programs were combined to develop the ensemble-learning model (ELM), which was externally validated. The strongest predictive factors for APA were serum potassium (s-K) at first visit, s-K after medication, plasma aldosterone concentration, aldosterone-to-renin ratio, and potassium supplementation dose. The average performance of the screening model had an AUC of 0.899; the confirmatory test model had an AUC of 0.913. In the external validation, the AUC was 0.964 in the screening model using an APA probability of 0.17. The clinical findings at screening predicted the diagnosis of APA with high accuracy. This novel algorithm can support the PA practice in primary care settings and prevent potentially curable APA patients from falling outside the PA diagnostic flowchart.
Evaluation of polyanionic cyclodextrins as high affinity binding scaffolds for fentanyl
Authors: Mayer Brian P. , Kennedy Daniel J. , Lau Edmond Y. , Valdez Carlos A. 
Published: February 2023

AbstractCyclodextrins (CDs) have been previously shown to display modest equilibrium binding affinities (Ka ~ 100–200 M-1) for the synthetic opioid analgesic fentanyl. In this work, we describe the synthesis of new CDs possessing extended thioalkylcarboxyl or thioalkylhydroxyl moieties and assess their binding affinity towards fentanyl hydrochloride. The optimal CD studied displays a remarkable affinity for the opioid of Ka = 66,500 M−1, the largest value reported for such an inclusion complex to date. One dimensional 1H Nuclear Magnetic Resonance (NMR) as well as Rotational Frame Overhauser Spectroscopy (2D-ROESY) experiments supported by molecular dynamics (MD) simulations suggest an unexpected binding behavior, with fentanyl able to bind the CD interior in one of two distinct orientations. Binding energies derived from the MD simulations work correlate strongly with NMR-derived affinities highlighting its utility as a predictive tool for CD candidate optimization. The performance of these host molecules portends their utility as platforms for medical countermeasures for opioid exposure, as biosensors, and in other forensic science applications.
Nasopharyngeal SARS-CoV-2 may not be dispersed by a high-flow nasal cannula
Authors: Suzuki Tetsuya , Morioka Shinichiro , Yamamoto Kei , Saito Sho , Iida Shun , Teruya Katsuji , Takasaki Jin , Hojo Masayuki , Hayakawa Kayoko , Kutsuna Satoshi , Miyamoto Sho , Ozono Seiya , Suzuki Tadaki , Kodama Eiichi N. , Ohmagari Norio 
Published: February 2023

AbstractA high-flow nasal cannula (HFNC) therapy plays a significant role in providing respiratory support to critically ill patients with coronavirus disease 2019 (COVID-19); however, the dispersion of the virus owing to aerosol generation is a matter of concern. This study aimed to evaluate if HFNC disperses the virus into the air. Among patients with COVID-19 admitted to private rooms with controlled negative pressure, we enrolled those admitted within 10 days of onset and requiring oxygenation through a conventional nasal cannula or HFNC therapy. Of the 17 patients enrolled, we obtained 22 samples (11 in the conventional nasal cannula group and 11 in the HFNC group). Viral RNA was detected in 20 nasopharyngeal swabs, and viable viruses were isolated from three nasopharyngeal swabs. Neither viral RNA nor viable virus was detected in the air sample at 0.5 m regardless of the oxygen-supplementation device. We detected viral RNA in two samples in the conventional nasal cannula group but not in the HFNC therapy group in gelatin filters located 3 m from the patient and the surface of the ventilation. This study directly demonstrated that despite viral RNA detection in the nasopharynx, viruses may not be dispersed by HFNC therapy. This warrants further research to determine if similar results can be obtained under different conditions.
The importance of temporal-fine structure to perceive time-compressed speech with and without the restoration of the syllabic rhythm
Authors: Gransier Robin , Peeters Sara , Wouters Jan 
Published: February 2023

AbstractIntelligibility of time-compressed (TC) speech decreases with increasing speech rate. However, intelligibility can be restored by ‘repackaging’ the TC speech by inserting silences between the syllables so that the original ‘rhythm’ is restored. Although restoration of the speech rhythm affects solely the temporal envelope, it is unclear to which extent repackaging also affects the perception of the temporal-fine structure (TFS). Here we investigate to which extent TFS contributes to the perception of TC and repackaged TC speech in quiet. Intelligibility of TC sentences with a speech rate of 15.6 syllables per second (sps) and the repackaged sentences, by adding 100 ms of silence between the syllables of the TC speech (i.e., a speech rate of 6.1 sps), was assessed for three TFS conditions: the original TFS and the TFS conveyed by an 8- and 16-channel noise vocoder. An overall positive effect on intelligibility of both the repackaging process and of the amount of TFS available to the listener was observed. Furthermore, the benefit associated with the repackaging TC speech depended on the amount of TFS available. The results show TFS contributes significantly to the perception of fast speech even when the overall rhythm/envelope of TC speech is restored.
Investigation of SAMD1 ablation in mice
Authors: Campbell Bruce , Weber Lisa M. , Engle Sandra J. , Ozolinš Terence R. S. , Bourassa Patricia , Aiello Robert , Liefke Robert 
Published: February 2023

AbstractSAM domain-containing protein 1 (SAMD1) has been implicated in atherosclerosis, as well as in chromatin and transcriptional regulation, suggesting a versatile and complex biological function. However, its role at an organismal level is currently unknown. Here, we generated SAMD1−/− and SAMD1+/− mice to explore the role of SAMD1 during mouse embryogenesis. Homozygous loss of SAMD1 was embryonic lethal, with no living animals seen after embryonic day 18.5. At embryonic day 14.5, organs were degrading and/or incompletely developed, and no functional blood vessels were observed, suggesting failed blood vessel maturation. Sparse red blood cells were scattered and pooled, primarily near the embryo surface. Some embryos had malformed heads and brains at embryonic day 15.5. In vitro, SAMD1 absence impaired neuronal differentiation processes. Heterozygous SAMD1 knockout mice underwent normal embryogenesis and were born alive. Postnatal genotyping showed a reduced ability of these mice to thrive, possibly due to altered steroidogenesis. In summary, the characterization of SAMD1 knockout mice suggests a critical role of SAMD1 during developmental processes in multiple organs and tissues.
Cross-municipality migration and spread of tuberculosis in South Africa
Authors: Fofana Abdou M. , Moultrie Harry , Scott Lesley , Jacobson Karen R. , Shapiro Anne N. , Dor Graeme , Crankshaw Beth , Silva Pedro Da , Jenkins Helen E. , Bor Jacob , Stevens Wendy S. 
Published: February 2023

AbstractHuman migration facilitates the spread of infectious disease. However, little is known about the contribution of migration to the spread of tuberculosis in South Africa. We analyzed longitudinal data on all tuberculosis test results recorded by South Africa’s National Health Laboratory Service (NHLS), January 2011–July 2017, alongside municipality-level migration flows estimated from the 2016 South African Community Survey. We first assessed migration patterns in people with laboratory-diagnosed tuberculosis and analyzed demographic predictors. We then quantified the impact of cross-municipality migration on tuberculosis incidence in municipality-level regression models. The NHLS database included 921,888 patients with multiple clinic visits with TB tests. Of these, 147,513 (16%) had tests in different municipalities. The median (IQR) distance travelled was 304 (163 to 536) km. Migration was most common at ages 20–39 years and rates were similar for men and women. In municipality-level regression models, each 1% increase in migration-adjusted tuberculosis prevalence was associated with a 0.47% (95% CI: 0.03% to 0.90%) increase in the incidence of drug-susceptible tuberculosis two years later, even after controlling for baseline prevalence. Similar results were found for rifampicin-resistant tuberculosis. Accounting for migration improved our ability to predict future incidence of tuberculosis.
Hydrodynamic Kelvin–Helmholtz instability on metallic surface
Authors: Wang Xi , Hu Xiao-Mian , Wang Sheng-Tao , Pan Hao , Yin Jian-Wei 
Published: February 2023

AbstractKelvin–Helmholtz instability on metallic surface is relevant to intense oblique impact in many physical processes such as explosive welding, Inertial Confinement Fusion and planetary impact events. Evolution of instability results in the formation of wavy morphology leading to material bonding or even mixing. However, mostly due to lack method to describe the dynamic behavior, instability mechanism controlled by elastoplastic properties of metal remains elusive. Here, we introduce a theory to reveal the evolution characteristics aroused by tangential velocity. Our simulations find that the unstable metallic surfaces exhibit amplitude growth and tangential motion by overcoming the depression of yield strength to generate wavy morphology. For diverse loading velocities, corrugated surfaces and material properties, an instability boundary distinguishes all unstable evolutions. Our analytical method with scale-independent variables reproducing numerical findings reveals plentiful characteristics of instability in strength materials. For designed loading velocities and material in oblique impact experiment in laboratory, the property of corrugated surfaces becomes an important factor to determine instability evolution.
Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry
Authors: Brodeur Alyssa , Winter Alexandre , Roy Vincent , Touzel Deschênes Lydia , Gros-Louis François , Ruel Jean 
Published: February 2023

AbstractEntirely biological human tissue-engineered blood vessels (TEBV) were previously developed for clinical use. Tissue-engineered models have also proven to be valuable tools in disease modelling. Moreover, there is a need for complex geometry TEBV for study of multifactorial vascular pathologies, such as intracranial aneurysms. The main goal of the work reported in this article was to produce an entirely human branched small-caliber TEBV. The use of a novel spherical rotary cell seeding system allows effective and uniform dynamic cell seeding for a viable in vitro tissue-engineered model. In this report, the design and fabrication of an innovative seeding system with random spherical 360° rotation is described. Custom made seeding chambers are placed inside the system and hold Y-shaped polyethylene terephthalate glycol (PETG) scaffolds. The seeding conditions, such as cell concentration, seeding speed and incubation time were optimized via count of cells adhered on the PETG scaffolds. This spheric seeding method was compared to other approaches, such as dynamic and static seeding, and clearly shows uniform cell distribution on PETG scaffolds. With this simple to use spherical system, fully biological branched TEBV constructs were also produced by seeding human fibroblasts directly on custom-made complex geometry PETG mandrels. The production of patient-derived small-caliber TEBVs with complex geometry and optimized cellular distribution all along the vascular reconstructed may be an innovative way to model various vascular diseases such as intracranial aneurysms.
Digestive enzymes and sphingomyelinase D in spiders without venom (Uloboridae)
Authors: Valladão Rodrigo , Neto Oscar Bento Silva , de Oliveira Gonzaga Marcelo , Pimenta Daniel Carvalho , Lopes Adriana Rios 
Published: February 2023

AbstractSpiders have distinct predatory behaviours selected along Araneae’s evolutionary history but are mainly based on the use of venom for prey paralysis. Uloboridae spiders have lost their venom glands secondarily during evolution. Because of this, they immobilise their prey by extensively wrapping, and digestion starts with the addition of digestive fluid. During the extra-oral digestion, the digestive fluid liquefies both the prey and the AcSp2 spidroins from the web fibres. Despite the efficiency of this process, the cocktail of enzymes involved in digestion in Uloboridae spiders remains unknown. In this study, the protein content in the midgut of Uloborus sp. was evaluated through enzymatic, proteomic, and phylogenetic analysis. Hydrolases such as peptidases (endo and exopeptidases: cysteine, serine, and metallopeptidases), carbohydrases (alpha-amylase, chitinase, and alpha-mannosidase), and lipases were biochemically assayed, and 50 proteins (annotated as enzymes, structural proteins, and toxins) were identified, evidencing the identity between the digestive enzymes present in venomous and non-venomous spiders. Even enzymes thought to be unique to venom, including enzymes such as sphingomyelinase D, were found in the digestive system of non-venomous spiders, suggesting a common origin between digestive enzymes and enzymes present in venoms. This is the first characterization of the molecules involved in the digestive process and the midgut protein content of a non-venomous spider.
Mixture effects of thiamethoxam and seven pesticides with different modes of action on honey bees (Aplis mellifera)
Authors: Li Wenhong , Lv Lu , Wang Yanhua , Zhu Yu-Cheng 
Published: February 2023

AbstractEven though honey bees in the field are routinely exposed to a complex mixture of many different agrochemicals, few studies have surveyed toxic effects of pesticide mixtures on bees. To elucidate the interactive actions of pesticides on crop pollinators, we determined the individual and joint toxicities of thiamethoxam (THI) and other seven pesticides [dimethoate (DIM), methomyl (MET), zeta-cypermethrin (ZCY), cyfluthrin (CYF), permethrin (PER), esfenvalerate (ESF) and tetraconazole (TET)] to honey bees (Aplis mellifera) with feeding toxicity test. Results from the 7-days toxicity test implied that THI elicited the highest toxicity with a LC50 data of 0.25 (0.20–0.29) μg mL−1, followed by MET and DIM with LC50 data of 4.19 (3.58–4.88) and 5.30 (4.65–6.03) μg mL−1, respectively. By comparison, pyrethroids and TET possessed relatively low toxicities with their LC50 data from the range of 33.78 (29.12–38.39) to 1125 (922.4–1,442) μg mL−1. Among 98 evaluated THI-containing binary to octonary mixtures, 29.59% of combinations exhibited synergistic effects. In contrast, 18.37% of combinations exhibited antagonistic effects on A. mellifera. Moreover, 54.8% pesticide combinations incorporating THI and TET displayed synergistic toxicities to the insects. Our findings emphasized that the coexistence of several pesticides might induce enhanced toxicity to honey bees. Overall, our results afforded worthful toxicological information on the combined actions of neonicotinoids and current-use pesticides on honey bees, which could accelerate farther comprehend on the possible detriments of other pesticide mixtures in agro-environment.
Comparison of different software for processing physical activity measurements with accelerometry
Authors: Verhoog Sanne , Gubelmann Cédric , Bano Arjola , Muka Taulant , Franco Oscar H. , Marques-Vidal Pedro 
Published: February 2023

AbstractSeveral raw-data processing software for accelerometer-measured physical activity (PA) exist, but whether results agree has not been assessed. We examined the agreement between three different software for raw accelerometer data, and associated their results with cardiovascular risk. A cross-sectional analysis conducted between 2014 and 2017 in 2693 adults (53.4% female, 45–86 years) living in Lausanne, Switzerland was used. Participants wore the wrist-worn GENEActive accelerometer for 14 days. Data was processed with the GENEActiv manufacturer software, the Pampro package in Python and the GGIR package in R. For the latter, two sets of thresholds “White” and “MRC” defining levels of PA and two versions (1.5–9 and 1.11–1) for the “MRC” threshold were used. Cardiovascular risk was assessed using the SCORE risk score. Time spent (mins/day) in stationary, light, moderate and vigorous PA ranged from 633 (GGIR-MRC) to 1147 (Pampro); 93 (GGIR-White) to 196 (GGIR-MRC); 19 (GGIR-White) to 161 (GENEActiv) and 1 (GENEActiv) to 26 (Pampro), respectively. Spearman correlations between results ranged between 0.317 and 0.995, while concordance coefficients ranged between 0.035 and 0.968. With some exceptions, the line of perfect agreement was not in the 95% confidence interval of the Bland–Altman plots. Compliance to PA guidelines varied considerably: 99.8%, 98.7%, 76.3%, 72.6% and 50.2% for Pampro, GENEActiv, GGIR-MRC v.1.11–1, GGIR-MRC v.1.4–9 and GGIR-White, respectively. Cardiovascular risk decreased with increasing time spent in PA across most software packages. We found large differences in PA estimation between software and thresholds used, which makes comparability between studies challenging.
A proof of principle study using radiopharmaceuticals to quantify and localize container-content interactions in medical syringes
Authors: Dupire C. , Chennell P. , Pereira B. , Courtiol N. , Buj S. , Cueff R. , Crauste-Manciet S. , Sautou V. , Morgat C. 
Published: February 2023

AbstractThe sorption of drugs onto their contents is a known phenomenon that is difficult to analyse precisely. The purpose of this study was to present a non-invasive method for locating and quantifying sorption phenomena using radiopharmaceuticals. Radiopharmaceutical are medicines armed with a radionuclide enabling quantification and imaging using dedicated scanners. The sorption of nine different radiopharmaceuticals on 2- and 3-part syringes was investigated. These syringes were filled with the studied radiopharmaceutical solutions and stored immobile for 3 h. At different times ranging from 0 to 180 min, 10 µL were taken from the syringes and the radioactivity of these samples was determined by a gamma counter. 5 radiopharmaceuticals exhibited no significant sorption at any time point in both 2 and 3-parts syringes, but 4 radiopharmaceuticals exhibited sorption losses varying from 20 to 33% after 3 h contact with 3-part-syringes, but no sorption on 2-part syringes at any time point. [99mTc]Tc-tetrofosmine Single Photon Emission Computed Tomography/Computed Tomography imaging indicated clearly that the interactions were located on the rubber plunger of the 3-part-syringes. The specific nature of radiopharmaceuticals allowed their use as an innovative method to quantify and localize drug sorption phenomena.
Iron oxide/hydroxide–nitrogen doped graphene-like visible-light active photocatalytic layers for antibiotics removal from wastewater
Authors: Ivan R. , Popescu C. , Antohe V. A. , Antohe S. , Negrila C. , Logofatu C. , del Pino A. Pérez , György E. 
Published: February 2023

AbstractHybrid layers consisting of Fe oxide, Fe hydroxide, and nitrogen doped graphene-like platelets have been synthesized by an eco-friendly laser-based method for photocatalytic applications. The complex composite layers show high photodecomposition efficiency towards degradation of antibiotic molecules under visible light irradiation. The photodecomposition efficiency was investigated as a function of relative concentrations of base materials, Fe oxide nanoparticles and graphene oxide platelets used for the preparation of target dispersions submitted to laser irradiation. Although reference pure Fe oxide/Fe hydroxide layers have high absorption in the visible spectral region, their photodecomposition efficiency is negligible under the same irradiation conditions. The high photocatalytic decomposition efficiency of the nanohybrid layer, up to 80% of the initial antibiotic molecules was assigned to synergistic effects between the constituent materials, efficient separation of the electron–hole pairs generated by visible light irradiation on the surface of Fe oxide and Fe hydroxide nanoparticles, in the presence of conducting graphene-like platelets. Nitrogen doped graphene-like platelets contribute also to the generation of electron–hole pairs under visible light irradiation, as demonstrated by the photocatalytic activity of pure, reference nitrogen doped graphene-like layers. The results also showed that adsorption processes do not contribute significantly to the removal of antibiotic molecules from the test solutions. The decrease of the antibiotic concentration under visible light irradiation was assigned primarily to photocatalytic decomposition mechanisms.