bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of microbial eukaryote community to the oligotrophic waters of the Gulf of Mexico: a plausible scenario for warm and stratified oceans
Authors: Sidón-Ceseña K. , Martínez-Mercado M.A. , Chong-Robles J. , Ortega-Saad Y. , Camacho-Ibar V.F. , Linacre L. , Lago-Lestón A. 
Published: July 2023

SummaryIn oligotrophic environments, interactions among eukaryotic microorganisms are highly complex. In the Gulf of Mexico (GoM), the Loop Current intensifies in summer and supplies the Gulf with warm and oligotrophic waters. However, mesoscale eddies within the GoM create favorable conditions for biological productivity by bringing nutrient-rich water to the subsurface layer. This study aimed to determine the structure, variability, and ecological roles of the protist in the mixed layer (ML) and deep chlorophyll maximum (DCM), representing the first V9-18S rRNA survey studying the protist community from the Southern GoM. Results revealed different assemblages between the ML and DCM. In the ML, species abundance was highly and positively correlated with temperature but negatively correlated with the nitrate concentration, whereas the opposite pattern was observed in the DCM. Alveolata represented ∼60% in both the ML and DCM, while Haptophytes and MAST dominated the ML, and Pelagophytes and Radiolarians dominated the DCM. Interestingly,Ostreococcusabundance increased under upwelling conditions suggesting that it may act as an indicator of the vertical nitrate flux and that picoeukaryotes respond to this instead of diatoms. Finally, our analyses revealed high levels of competition, parasitism, and predation with a high proportion of self-exclusion relationship (30%) in both depths.
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1
Authors: Carmona Bruno , Camelo Carolina , Mehraz Manon , Lemullois Michel , Faria Mariana Lince , Coyaud Étienne , Marinho H. Susana , Gonçalves João , Nolasco Sofia , Pinto Francisco , Raught Brian , Tassin Anne-Marie , Koll France , Soares Helena 
Published: July 2023

ABSTRACTCentrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures inParamecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks.
Challenges in identifying mRNA transcript starts and ends from long-read sequencing data
Authors: Calvo-Roitberg Ezequiel , Daniels Rachel F. , Pai Athma A. 
Published: July 2023

ABSTRACTLong-read sequencing (LRS) technologies have the potential to revolutionize scientific discoveries in RNA biology, especially by enabling the comprehensive identification and quantification of full length mRNA isoforms. However, inherently high error rates make the analysis of long-read sequencing data challenging. While these error rates have been characterized for sequence and splice site identification, it is still unclear how accurately LRS reads represent transcript start and end sites. Here, we systematically assess the variability and accuracy of mRNA terminal ends identified by LRS reads across multiple sequencing platforms. We find substantial inconsistencies in both the start and end coordinates of LRS reads spanning a gene, such that LRS reads often fail to accurately recapitulate annotated or empirically derived terminal ends of mRNA molecules. To address this challenge, we introduce an approach to condition reads based on empirically derived terminal ends and identified a subset of reads that are more likely to represent full-length transcripts. Our approach can improve transcriptome analyses by enhancing the fidelity of transcript terminal end identification, but may result in lower power to quantify genes or discover novel isoforms. Thus, it is necessary to be cautious when selecting sequencing approaches and/or interpreting data from long-read RNA sequencing.
DSPE-PEG-CeO2inhibits tumor cell proliferation by inducing oxidative stress and cell death
Authors: Qin SiHao , Wang Xueyao , Li Huan , Jiang YanFang 
Published: July 2023

SUMMARYCeO2can consume intracellular reducing power, but its poor biocompatibility limits its application in tumor therapy. In this study, we designed and synthesized DSPE-PEG-CeO2, which is easy to enter cells, and showed that DSPE-PEG-CeO2can induce the increase of ROS levels in tumor cells, thereby inducing tumor cell apoptosis and DNA damage. This suppresses the appearance and development of tumor cells. This provides a new idea for the application of inorganic nanomaterials.
Downregulation of lysosomal trafficking in ARPE19 cells leads to decreased transfection efficiency at high passage
Authors: Hood Erika M.S. , Jones Lipinski Rachel A. , Lipinski Daniel M. 
Published: July 2023

ABSTRACTPURPOSEARPE19 cells are a commonly used cell culture model for the study of retinal pigment epithelial cell biology and pathologies. However, numerous studies have demonstrated that ARPE19 undergo morphologic, transcriptomic and genomic alterations over time and with increasing passage number. Herein, we explore the mechanisms underlying increased resistance to the delivery of exogenous genetic material via transfection in ARPE19 cells using mass spectrometry.METHODSARPE19 cells (N=5 wells/reagent) were seeded in 6-well plates at passages 24 through 30. At 70% confluency an mCherry reporter construct was delivered via transfection using Lipofectamine 3000, Lipofectamine LTX, Lipofectamine Stem, or PEI (polyethylenimine) reagents. After 72 hours, transfection efficiency was quantified by fluorescence microscopy and flow cytometry. Mass spectrometry and immunofluorescence of ARPE19 cells were performed at passages 24 and 30 to evaluate altered protein synthesis and localization between passage numbers.RESULTSARPE19 transfection showed a maximum transfection efficiency of 32.4% at P26 using Lipofectamine 3000 reagent. All lipofectamine based reagents demonstrated statistically significant decreases in transfection efficiency between passages 24 and 30. Mass spectrometry analysis revealed 18 differentially expressed proteins, including down-regulation of clathrin light chain B (CLTB) and legumain (LGMN) that was confirmed via immunofluorescence imaging, which indicated altered intracellular localization.CONCLUSIONSARPE19 cells demonstrate passage number dependent changes in lipofectamine-based transfection efficiency. Mass spectrometry and immunofluorescence indicates the observed decrease in transfection efficiency involves the dysregulation of endocytosis and intracellular endolysosomal trafficking at later passages.TRANSLATIONAL RELEVANCEThis study contributes to mounting evidence for changes in ARPE19 cell physiology with increasing passage number. This information is of value for the continued use of ARPE19 cells as a model system for RPE biology and the development of therapeutics.
Flatness promotes modernity: logo flatness and consumers' perception of brand image
Authors: Peng Luluo , Wei Yuting , Zhang Xiaodan , Wang Danping 
Published: July 2023

PurposeThe brand logo, as a fundamental element of marketing communications, serves as a crucial visual representation of a brand. In the current era of mobile Internet, logo flatness has become a new trend in practice. However, there remains a scarcity of research that explores the effects of logo flatness on consumer perceptions and brand attitudes.Design/methodology/approachAcross four studies, using both observational analyses of real brands and experimental manipulations of fictitious brands, the authors examined the impact of logo flatness on consumer perceptions and brand attitudes.FindingsResults show that logo flatness promotes the perception of modernity due to the simplicity it presents. Consumers will evaluate the brand more positively when their perception of the logo association is congruent with the brand image. Notably, traditional brands using skeuomorphic logos and modern brands employing flat logos can effectively enhance consumers' brand attitudes.Practical implicationsThe findings of this study have significant implications for businesses seeking to enhance consumers' brand attitude and foster brand renewal through the strategic selection and design of logos that align with their brand image.Originality/valueThis study provides a theoretical and empirical test of the influence of logo flatness on consumers' perception of brand image, thereby enriching the existing research on brand management.
Firm efficiency and corporate performance: the moderating role of controlling shareholders
Authors: Thien Nguyen Huu , Asif Jawad , Kweh Qian Long , Ting Irene Wei Kiong 
Published: July 2023

PurposeThis study analyses the effects of firm efficiency on firm performance and how controlling shareholders moderate the link between the two variables.Design/methodology/approachThis study employs data envelopment analysis to estimate firm efficiency and the panel regression method to assess the hypothesised relationships among 1,295 firm-year observations of publicly listed firms in Malaysia from 2015 to 2019.FindingsThe results indicate that firm efficiency (technical efficiency, pure technical efficiency and scale efficiency) has mixed relationships with firm performance (return on assets, market-to-book ratio and operating cash flows), all of which are being moderated by controlling shareholdings.Practical implicationsThis study highlights the importance of assessing firm efficiency as the key success factor for improving firm performance. Industrial managers should manage efficiently their resources or operating costs in achieving their corporate financial goals. Moreover, this study notes the presence of controlling shareholders, who can be either self-interested or company goal aligned.Originality/valueThis study suggests becoming efficient in transforming inputs into outputs is a prerequisite before investigating accrual-based and cash-based firm performance measures, and the presence of controlling shareholders matters in these regards.
Performance variability, prior failure experience and entrepreneurs’ physical health
Authors: Lin Song , Zhang Ru 
Published: July 2023

Purpose
Past studies have mainly concentrated on the impact of stress and self-employment on physical health. However, little research has paid attention to firm-level determinants of entrepreneurs’ physical health. This study aims to investigate the relationship between performance variability and entrepreneurs’ physical health as well as the moderating effect of prior failure experience.

Design/methodology/approach
This study uses data drawn from 255 start-ups across the Bohai Economic Rim in China over a period of three years (2015–2017). The results are still robust after several robustness checks.

Findings
Results show that performance variability is positively related to the probability of entrepreneurs showing poor health. This confirms that performance variability has an adverse effect on entrepreneurs’ physical health. Moreover, this relationship is moderated by the prior failure experience of entrepreneurs.

Practical implications
First, entrepreneurs should gain more information about the firm’s daily operation to reduce the potential threat of performance variability. Second, it is imperative for entrepreneurs to build a stable relationship with their stakeholders to avoid the potential costs related to performance variability. Finally, entrepreneurs should take health consequences into consideration when making strategic decisions.

Originality/value
First, this paper contributes to the studies on the antecedents of entrepreneurs’ physical health by introducing a firm-level determinant (i.e. performance variability). The findings enhance the understanding of the association between entrepreneurs and new ventures. Second, this paper also enriches the extant literature on the outcomes of performance variability. Finally, this paper attempts to offer new insights into prior failure experience by establishing its moderating effect on the performance–health relationship.
Motivating mobile payment adoption during global pandemic: insights from protection motivation and theory of planned behavior
Authors: Hazarika Bidyut , Shrivastava Utkarsh , Singh Vivek Kumar , Rea Alan 
Published: July 2023

Purpose
The COVID-19 pandemic has had far-reaching effects on society and will continue to be a subject of study for researchers in the years to come. Businesses have implemented technologies that reduce reliance on physical currencies, such as e-commerce sites and contactless payments. This study aims to examine the users’ attitudes and behaviors toward mobile payments. The focus is on identifying the most effective techniques and approaches that businesses can use to encourage user adoption of mobile payments.

Design/methodology/approach
This study uses survey data from 396 active mobile payment users across the mid-west region of the USA to test the proposed hypothesis. The snowball sampling approach is used to sample the participants for the data collection. This study uses partial least squares structural equation modeling to test the ten hypotheses proposed in this study.

Findings
This study finds that organizational commitment and privacy customization can significantly overcome users’ protective attitudes toward mobile payments during the pandemic. In addition, providing users with privacy customization options can significantly encourage self-disclosure, which is crucial for transaction authentication and fraud detection.

Originality/value
Envisioned in the backdrop of the COVID pandemic, this is one of the earliest studies investigating the role of privacy customization, self-disclosure and organizational commitment on mobile payment adoption.
Empowering vs empowered: factors shaping women’s workplace empowerment in the Qatari public sector
Authors: Naguib Rabia , Madeeha Muznah 
Published: July 2023

PurposeDespite several policies in the Arab Gulf States aimed at promoting women’s empowerment through employment, women’s career progress has not met the expected gains. Workplace empowerment is a critical aspect of women’s economic empowerment. Therefore, this paper aims to investigate the factors that contribute to workplace empowerment for women in the Qatari public sector.Design/methodology/approachThis research uses a mixed-methods approach to explore workplace empowerment among female civil servants in the State of Qatar. The study combines surveys (N= 310) and interviews (N= 30) and uses an inductive thematic approach that considers women’s narratives as the primary source of knowledge construction.FindingsThe authors’ findings strongly suggest that perception-related factors have a more significant impact on workplace empowerment than structural ones. The results indicate that feelings of disempowerment are influenced by perceptions of gender-based discrimination, poor relationships with supervisors and dissatisfaction with work–life balance. Women feel empowered when they have access to decision-making opportunities and perceive that their workplace supports their professional growth and advancement.Research limitations/implicationsAlthough this paper focuses solely on women’s perceptions, additional research is necessary to compare the experiences of both men and women regarding workplace empowerment. While individual and organizational factors were examined in this paper, future studies should also consider societal factors. The results highlight the importance of equal and supportive organizational practices and cultures to foster empowerment among women in the workplace, providing valuable insights for policymakers.Originality/valueThis paper addresses a critical research gap on the intersection of gender, work and management in the Middle East. It responds to the need for more diverse contextual research on Arab women’s work experiences and provides methodological diversity by using an exploratory, mixed-methods design with a grounded approach. The study highlights the interaction between structural and psychological factors, emphasizing the gap between policies and resources and women’s lived experiences and perceptions of workplace empowerment.
A gender-oriented analysis of digital skills and ICT use intensity in tourism companies in Extremadura (Spain)
Authors: Sánchez-Rivero Marcelino , Gutiérrez-Fernández Milagros , Fernández-Torres Yakira , Gallego-Sosa Clara 
Published: July 2023

Purpose
This study aims to use a novel approach, focusing on the manager’s gender, to explore whether it acts as a differentiator in the following aspects of tourist accommodation companies in Extremadura (Spain): the level of information and communication technology (ICT) specialisation of employees, managers’ knowledge of ICTs and the social media and online tourism platform use intensity of managers.

Design/methodology/approach
A questionnaire was sent to 238 accommodation companies. The data collected from the questionnaire were analysed using statistical inference techniques and linear and logistic regression.

Findings
In general, ICT specialist profiles are more common amongst the employees of male-led companies. Male managers also use Booking and analyse online feedback more intensively. There appear to be no gender-based differences in terms of the ICT knowledge of managers.

Practical implications
These results highlight issues of major practical interest for the sector’s managers and decision makers, especially in Extremadura. They reveal the digital divide in certain aspects between men- and women-led firms in Extremadura. This finding has important consequences for the sector in terms of competitiveness. It highlights the need to continue working to eradicate gender gaps in digital settings.

Originality/value
The study shows the role of the manager’s gender as a differentiating factor in terms of the existence of specialist ICT profiles and ICT use intensity in tourism companies. To the best of the authors’ knowledge, this study provides the first evidence of such a finding for the tourism sector in general, as well as for the specific case of a rural destination such as Extremadura.
Keeping warm: racial disparities of warmth judgments in the service industry
Authors: Lee Lindsey , Yu Heyao , Jolly Phillip M. 
Published: July 2023

Purpose
People develop general stereotypes and beliefs about how people look and behave, and discrepancies in perceptions of warmth and competence can lead to incongruence in expected behaviors during service interactions. These perceptions can contribute to negative outcomes for employees; therefore, this study aims to examine how perceptions of warmth affect both customer reactions to employees who are Asian and the work stress, strain and attitudes of employees who are Asian toward their jobs.

Design/methodology/approach
To investigate the phenomenon, the authors conducted three studies. The first study examined whether occupational-racial stereotypes influence customers’ evaluation of employees’ service recovery performance. The second study temporally examined whether employees who are Asian engage in more emotional labor strategies to counter these occupational-racial stereotypes and as a result experience more negative outcomes. And a third study investigated the cumulative effects of negative perceptions on employee outcomes.

Findings
The results suggest employees in the hospitality industry who are Asian are perceived as less warm during service failure interactions and, thus, must work harder to regulate their emotions. As a result, employees who are Asian reported experiencing more negative outcomes, supporting the notion that occupational-racial stereotypes contribute to racial disparity and act as challenges and barriers for employees in the service industry.

Research limitations/implications
These findings emphasize the need to understand and address occupational-racial stereotypes for employees who are Asian to mitigate racial disparities in the service industry. Organizations can promote diverse work climates, provide emotional support and foster high-quality relationships to support employee well-being and satisfaction.

Originality/value
The results provide insight into how stereotypical warmth perceptions of employees who are Asian may affect the demands they face when engaging in service recovery, and how these increased demands may reduce the quality of their work experiences.
Asymmetric new Keynesian Phillips curve for Mexico, 2005Q1–2022Q4
Authors: Loría Eduardo , Tirado Cossío Raúl Antonio 
Published: July 2023

Purpose
The labor market responds in a differentiated manner during recessions and expansions, and it is of vital importance to know the magnitude asymmetries. The purpose of this paper is to evaluate the effects of the disinflationary monetary policy (2005Q1–2022Q4) through the sacrifice rate measured in terms of unemployment and rate of critical labor conditions (RCLC) with nonlinear auto regressive distributed lag (NLARDL; Shin et al., 2014), which allows to efficiently estimate asymmetric effects in short and long terms in the presence of variables of different integration orders.

Design/methodology/approach
The authors estimate an asymmetric accelerationist Phillips curve, augmented with labor precariousness for Mexico (2005Q1–2022Q4) following the NLARDL approach (Shin et al., 2014).

Findings
The authors prove that the increase in the unemployment gap has greater disinflationary effects than the RCLC in both the short and the long term; the expansionary phases of the business cycle, which reduce UGap, do not have inflationary effects either in the short or in the long run, but improvements in the labor market do, when RCLC is reduced; raising RCLC appears to have been the companies’ main survival strategy since 2015; and these asymmetries can generate a low unemployment trap with high and growing precariousness, with huge dynamic costs for well-being, economic growth, inequality and poverty.

Social implications
As labor precariousness grows, the implications are several both in the short and long run. In the short run, the most notorious example of the effects on workers has to do with unstable and insecure situations, that disrupt all their life planning options, and health issues. Bohle et al. (2004) found in the Organization for Economic Cooperation and Development countries that casual employees had less desirable and predictable working hours, greater work–life conflict and more associated health complaints than people with permanent jobs.

Originality/value
The approach includes the labor precariousness variable, which describes a new phenomenon in the labor market. Nowadays, workers are facing a new threat since firms are employing a new labor cost reduction strategy in which they do not lay off workers but rather paying them less, working them more hours, or reducing benefits. The asymmetries between the effects of precarity and unemployment can generate a poverty trap in the long run. This problem is, once again, of great relevance in the context of global high inflation.