bluuebunny/crossref_metadata_2025_split huggingface dataset:
A systematic review, umbrella review, and quality assessment on clinical translation of stem cell therapy for knee osteoarthritis: Are we there yet?
Authors: Shang Zhizhong , Wanyan Pingping , Zhang Baolin , Wang Mingchuan , Wang Xin 
Published: April 2023

Abstract
Background
The success of stem cell therapy for knee osteoarthritis (KOA) in preclinical animal models has accelerated the pace of clinical translation. However, it remains uncertain whether the current scientific evidence supports the clinical application of stem cells in treating KOA. A comprehensive evaluation of the safety and efficacy of stem cell therapies and scientific evidence quality is necessary.

Methods
Using “stem cells” and “knee osteoarthritis” as the search terms, several databases, including PubMed, Web of Science, Cochrane, Embase, and Clinicaltrials.gov, were searched on August 25, 2022, and updated on February 27, 2023. Clinical studies that reported adverse reactions (ARs) of stem cell therapy in KOA patients were included without limiting the type of studies. Quantitative systematic reviews of stem cell therapy for KOA that conducted meta-analysis were included. Two researchers conducted literature screening and data extraction independently, and the evidence quality was evaluated according to the Institute of Health Economics and AMSTAR 2 criteria.

Results
Fifty clinical studies and 13 systematic reviews/meta-analyses (SRs/MAs) were included. Nineteen ARs were reported in 50 studies, including five knee-related ARs, seven common ARs, and seven other ARs. Some studies reported over 10% prevalence of knee pain (24.5%; 95% CI [14.7%, 35.7%]), knee effusion (12.5%; 95% CI [4.8%, 22.5%]), and knee swelling (11.9%; 95% CI [3.5%, 23.5%]). Additionally, two studies have reported cases of prostate cancer and breast tumors, respectively. However, these two studies suggest that stem cell therapy does not bring significant ARs to patients. SRs/MAs results revealed that stem cell therapy relieved pain in patients over time but did not improve knee function. However, current clinical studies have limited evidence regarding study objectives, test designs, and patient populations. Similarly, SRs/MAs have inadequate evidence regarding study design, risk of bias assessment, outcome description, comprehensive discussion, and potential conflicts of interest.

Conclusions
The inefficacy of stem cells, the risk of potential complications, and the limited quality of evidence from current studies precluded any recommendation for using stem cell products in patients with KOA. Clinical translation of stem cell therapies remains baseless and should be cautiously approached until more robust evidence is available.
PROSPERO registration number: CRD42022355875.
Conducting separate reviews of benefits and harms could improve systematic reviews and meta-analyses
Authors: Mayo-Wilson Evan , Qureshi Riaz , Li Tianjing 
Published: April 2023

Abstract
Guidance for systematic reviews of interventions recommends both benefits and harms be included. Systematic reviews may reach conclusions about harms (or lack of harms) that are not true when reviews include only some relevant studies, rely on incomplete data from eligible studies, use inappropriate methods for synthesizing data, and report results selectively. Separate reviews about harms could address some of these problems, and we argue that conducting separate reviews of harms is a feasible alternative to current standards and practices. Systematic reviews of potential benefits could be organized around the use of interventions for specific health problems. Systematic reviews of potential harms could be broader, including more diverse study designs and including all people at risk of harms (who might use the same intervention to treat different health problems). Multiple reviews about benefits could refer to a single review of harms. This approach could improve the reliability, completeness, and efficiency of systematic reviews.
An interdisciplinary student-led multifaceted intervention addressing overuse of broad-spectrum antibiotics for patients with penicillin allergies
Authors: Banashefski Bryana , Henson Philip , David Navindra , Kok Hui Ting , Beerkens Frans J. , Shyu Margaret , Linker Anne S. , Tsega Surafel , Dunn Andrew , Fuller Risa 
Published: April 2023

Abstract
Background
Though 15% of hospitalized patients have a documented penicillin (PCN) allergy, fewer than 1% have an IgE-mediated reaction that necessitates avoidance of β-lactam antibiotics.

Objective
Our interdisciplinary team of medical and nursing students led and executed a two-pronged quality improvement intervention to reduce prescribing of non-β-lactam antibiotics (NBLs) for patients with reported PCN allergies. To the best of our knowledge, this is the first multidisciplinary student-led intervention aimed at educating providers on low-risk penicillin allergy and encouraging best antibiotic prescribing practices.

Design and participants
The intervention took place from June 2021 to February 2022. We developed and provided clinician education modules, including peer-to-peer information sharing and in-person small group discussions, as well as clinical decision support (CDS) strategies through the electronic medical record (EMR). The target population was attendings, residents, nurse practitioners, and physician assistants on the hospital medicine service at a large urban academic tertiary care center. We followed the SQUIRE 2.0 guidelines for reporting on quality improvement.

Main measures
Primary outcome measures included number of NBL prescriptions and use of nonspecific descriptors (e.g., “other” or “unknown”) for PCN allergy reaction type, and were compared with a pre-intervention period.

Key results
The percent of β-lactam prescriptions for patients with a PCN allergy after the intervention increased from 19 to 23% (p = 0.006). For patients with a low severity PCN allergy, the percent of β-lactam prescriptions increased from 20 to 28% (p = 0.001). There was a significant decrease in nonspecific PCN allergy reaction type from 23% in the pre-intervention period to 20% post-intervention (p = 0.012).

Conclusions
An intervention focused on educating prescribers and CDS strategies delivered through the EMR increased appropriate β-lactam prescribing for patients with a documented low-risk PCN allergy and reduced the use of nonspecific PCN allergy reaction type in EMR documentation.
Novel insights into the multifaceted roles of m6A-modified LncRNAs in cancers: biological functions and therapeutic applications
Authors: Tang Jinxin , Zhang Jinhui , Lu Yu , He Jieyu , Wang Hua , Liu Binfeng , Tu Chao , Li Zhihong 
Published: April 2023

AbstractN6-methyladenosine (m6A) is considered as the most common and important internal transcript modification in several diseases like type 2 diabetes, schizophrenia and especially cancer. As a main target of m6A methylation, long non-coding RNAs (lncRNAs) have been proved to regulate cellular processes at various levels, including epigenetic modification, transcriptional, post-transcriptional, translational and post-translational regulation. Recently, accumulating evidence suggests that m6A-modified lncRNAs greatly participate in the tumorigenesis of cancers. In this review, we systematically summarized the biogenesis of m6A-modified lncRNAs and the identified m6A-lncRNAs in a variety of cancers, as well as their potential diagnostic and therapeutic applications as biomarkers and therapeutic targets, hoping to shed light on the novel strategies for cancer treatment.
Effect of Er,Cr:YSGG laser with a side-firing tip on decontamination of titanium disc surface: an in vitro and in vivo study
Authors: Kottmann Lucia , Franzen Rene , Conrads Georg , Wolfart Stefan , Marotti Juliana 
Published: April 2023

Abstract
Purpose
To evaluate the effectiveness of an erbium, chromium:yttrium–scandium–gallium–garnet (Er,Cr:YSGG) laser with side-firing tip in decontamination of titanium (Ti) disc.

Methods
In the first test series, 29 Ti-discs were contaminated with Staphylococcus aureus and treated as follows: positive control (no treatment); Perioflow; Laser A (0.75 W, 100 Hz), Laser B (1.5 W, 30 Hz); Laser C (no radiation, 60% water); and Laser D (no radiation, 50% water). For bacterial quantification, colony forming units (CFU, vital cells only) and quantitative PCR (qPCR, vital and devital cells) were performed. In a second test series, 92 Ti-discs were used, contaminated with in vivo-grown biofilm and treated as follows: positive control (no treatment); Perioflow; Laser E (1.5 W, 30 Hz), and Laser F (no radiation, 50% water). Considering the different and unknown culture conditions, quantification of bacteria was performed by broad-spectrum bacterial qPCR only. Based on the assumption that all cells of an organism contain an equivalent complement of genetic information, genome equivalent (GE) determination ensured the detection of the different intact and semi-intact genomes, regardless of type of bacterial species and vitality, circumvent the inherent bias of cultures.

Results
The GE values were significantly reduced by all interventions in both test series, compared to the positive control group (p < 0.001). In the first test series with S. aureus as model organism, Perioflow yielded a lower GE than the Laser groups A–D (all p < 0.025). The number of CFUs was significantly reduced in the intervention groups compared to the positive control (p < 0.001), except for Laser A (p = 0.157) and Laser D (p = 0.393). In the second test series, none of the pairwise comparisons of the intervention conditions showed a significant difference (Perioflow vs. Laser E: p = 0.732; Perioflow vs. Laser F: p = 0.590; Laser E vs. Laser F: p = 0.379).

Conclusion
The Er,Cr:YSGG laser with side-firing tip and Perioflow were equally capable of effectively decontaminating a Ti-disc surface. It is assumed that the bacterial reduction was largely due to the mechanical effect of the air and water stream.

Graphical Abstract
Recommendations for progression criteria during external randomised pilot trial design, conduct, analysis and reporting
Authors: Mellor Katie , Albury Charlotte , Dutton Susan J , Eldridge Sandra , Hopewell Sally 
Published: April 2023

Abstract
Background
External randomised pilot trials aim to assess whether a future definitive Randomised Controlled Trial (RCT) is feasible. Prespecified progression criteria help guide the interpretation of pilot trial findings to decide whether, and how, a definitive RCT should be conducted. This commentary presents a set of proposed recommendations for progression criteria to guide researchers when (i) designing, (ii) conducting, (iii) analysing and (iv) reporting external randomised pilot trials.

Methods
Recommendations were developed following a mixed methods approach. This involved (i) a methodological review of pilot trial publications, (ii) a cross-sectional study of pilot trial research funding applications, (iii) qualitative interviews with pilot trial researchers and (iv) a survey of corresponding authors of identified pilot trial publications. Initial recommendations were refined following two consultation stakeholder workshops held in July 2022.
Recommendations for progression criteria for external randomised pilot trials:
i. Design: consider progression criteria from the earliest opportunity; map progression criteria to feasibility objectives; consider quantitative and qualitative interpretations of feasibility; provide justification; develop guidelines rather than rules; seek input from relevant stakeholders.
ii. Conduct: regularly monitor pilot trial data against progression criteria.
iii. Analysis: avoid considering each progression criterion in isolation; engage in discussion with relevant stakeholders; consider context and other factors external to the pilot trial; consider feasibility (can we?) and progression (will we?).
iv. Reporting: we propose a reporting checklist in relation to progression criteria and recommend reporting in a table format for clarity.

Conclusion
These recommendations provide a helpful resource for researchers to consider progression criteria at different stages of external randomised pilot trials. We have produced a simple infographic tool to summarise these recommendations for researchers to refer to. Further research is needed to evaluate whether these proposed recommendations should inform future development, or update, of established guidelines for the design, conduct, analysis and reporting of external randomised pilot trials.
Neutrophil-inspired photothermo-responsive drug delivery system for targeted treatment of bacterial infection and endotoxins neutralization
Authors: Li Chengnan , Gan Yingying , Li Zongshao , Fu Mengjing , Li Yuzhen , Peng Xinran , Yang Yongqiang , Tian Guo-bao , Yang Yi Yan , Yuan Peiyan , Ding Xin 
Published: April 2023

Abstract

Background

P. aeruginosa
, a highly virulent Gram-negative bacterium, can cause severe nosocomial infections, and it has developed resistance against most antibiotics. New therapeutic strategies are urgently needed to treat such bacterial infection and reduce its toxicity caused by endotoxin (lipopolysaccharide, LPS). Neutrophils have been proven to be able to target inflammation site and neutrophil membrane receptors such as Toll-like receptor-4 (TLR4) and CD14, and exhibit specific affinity to LPS. However, antibacterial delivery system based on the unique properties of neutrophils has not been reported.

Methods

A neutrophil-inspired antibacterial delivery system for targeted photothermal treatment, stimuli-responsive antibiotic release and endotoxin neutralization is reported in this study. Specifically, the photothermal reagent indocyanine green (ICG) and antibiotic rifampicin (RIF) are co-loaded into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NP-ICG/RIF), followed by coating with neutrophil membrane to obtain antibacterial delivery system (NM-NP-ICG/RIF). The inflammation targeting properties, synergistic antibacterial activity of photothermal therapy and antibiotic treatment, and endotoxin neutralization have been studied in vitro. A
P. aeruginosa
-induced murine skin abscess infection model has been used to evaluate the therapeutic efficacy of the NM-NP-ICG/RIF.

Results

Once irradiated by near-infrared lasers, the heat generated by NP-ICG/RIF triggers the release of RIF and ICG, resulting in a synergistic chemo-photothermal antibacterial effect against
P. aeruginosa
(~ 99.99% killing efficiency in 5 min). After coating with neutrophil-like cell membrane vesicles (NMVs), the nanoparticles (NM-NP-ICG/RIF) specifically bind to inflammatory vascular endothelial cells in infectious site, endowing the nanoparticles with an infection microenvironment targeting function to enhance retention time. Importantly, it is discovered for the first time that NMVs-coated nanoparticles are able to neutralize endotoxins. The
P. aeruginosa
murine skin abscess infection model further demonstrates the in vivo therapeutic efficacy of NM-NP-ICG/RIF.

Conclusion
The neutrophil-inspired antibacterial delivery system (NM-NP-ICG/RIF) is capable of targeting infection microenvironment, neutralizing endotoxin, and eradicating bacteria through a synergistic effect of photothermal therapy and antibiotic treatment. This drug delivery system made from FDA-approved compounds provides a promising approach to fighting against hard-to-treat bacterial infections.
High blood pressure and associated factors among HIV-infected young persons aged 13 to 25 years at selected health facilities in Rwenzori region, western Uganda, September–October 2021
Authors: Migisha Richard , Ario Alex Riolexus , Kadobera Daniel , Bulage Lilian , Katana Elizabeth , Ndyabakira Alex , Elyanu Peter , Kalamya Julius N. , Harris Julie R. 
Published: April 2023

Abstract
Background
High blood pressure (HBP), including hypertension (HTN), is a predictor of cardiovascular events, and is an emerging challenge in young persons. The risk of cardiovascular events may be further amplified among people living with HIV (PLHIV). We determined the prevalence of HBP and associated factors among PLHIV aged 13 to 25 years in Rwenzori region, western Uganda.

Methods
We conducted a cross-sectional study among PLHIV aged 13 to 25 years at nine health facilities in Kabarole and Kasese districts during September 16 to October 15, 2021. We reviewed medical records to obtain clinical and demographic data. At a single clinic visit, we measured and classified BP as normal (< 120/ < 80 mmHg), elevated (120/ < 80 to 129/ < 80), stage 1 HTN (130/80 to 139/89), and stage 2 HTN (≥ 140/90). We categorized participants as having HBP if they had elevated BP or HTN. We performed multivariable analysis using modified Poisson regression to identify factors associated with HBP.

Results
Of the 1,045 PLHIV, most (68%) were female and the mean age was 20 (3.8) years. The prevalence of HBP was 49% (n = 515; 95% confidence interval [CI], 46%–52%), the prevalence of elevated BP was 22% (n = 229; 95% CI, 26%–31%), and the prevalence of HTN was 27% (n = 286; 95% CI, 25%–30%), including 220 (21%) with stage 1 HTN and 66 (6%) with stage 2 HTN. Older age (adjusted prevalence ratio [aPR], 1.21; 95% CI, 1.01–1.44 for age group of 18–25 years vs. 13–17 years), history of tobacco smoking (aPR, 1.41; 95% CI, 1.08–1.83), and higher resting heart rate (aPR, 1.15; 95% CI, 1.01–1.32 for > 76 beats/min vs. ≤ 76 beats/min) were associated with HBP.

Conclusions
Nearly half of the PLHIV evaluated had HBP, and one-quarter had HTN. These findings highlight a previously unknown high burden of HBP in this setting’s young populations. HBP was associated with older age, elevated resting heart rate, and ever smoking; all of which are known traditional risk factors for HBP in HIV-negative persons. To prevent future cardiovascular disease epidemics among PLHIV, there is a need to integrate HBP/HIV management.
Comparative evaluation of temperate, subtropical, and tropical isolates of nucleopolyhedrovirus against tomato fruit borer, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)
Authors: Manzoor Saika , Gani Mudasir , Hassan Taskeena , Shafi Insha , Wani Fehim Jeelani , Mumtaz Shabnum , Eroglu Gozde Busra , Yaqoob Munazah , Mantoo Mohd Ayoob 
Published: April 2023

Abstract
Background
The tomato fruit borer, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), is a serious insect pest owing to its polyphagous nature, migrating long distances, greater capacity to develop resistance against insecticides and persistence in cropping areas. The comparative evaluation of temperate, sub-tropical and tropical H. armigera NPV (HearNPV) isolates was carried out to determine the potential of local and exotic HearNPV isolates for the management of H. armigera in Kashmir, India, having a temperate climate.

Results
Mortality of 2nd instar H. armigera larvae with three different HearNPV isolates at different concentrations ranged between 13.40 and 73.25%, with significant differences between the concentrations in each isolate under laboratory conditions. The median lethal concentration (LC50) values of HearNPV-IND-K, HearNPV-IND-J, and HearNPV-IND-B isolates against 2nd instar H. armigera larvae were 4.62 × 103, 5.99 × 104, and 7.24 × 104 OBs/larva at 10th day post inoculation, with significant differences among the isolates. In time response bioassays, the cumulative mortality (%) caused by median lethal concentration (LC50) of HearNPV-IND-K, HearNPV-IND-J and HearNPV-IND-B isolates over a period of 10 days was 50.33, 49.00 and 49.00%, respectively. Their median survival time (ST50) values against 2nd instar H. armigera larvae were 8.10, 8.94 and 9.50 days, respectively, with significant differences among the isolates. The results revealed that the LC50 and ST50 values of HearNPV-IND K isolate were significantly lower than HearNPV-IND-J and HearNPV-IND-B isolates. The cumulative mortality of H. armigera larvae with HearNPV-IND-K, HearNPV-IND-J and HearNPV-IND-B isolates at different concentrations ranged between 22.00–68.32, 20.54–55.17, 11.66–44.33%, respectively, on tomato crop under field conditions. The highest mortality rate of 68.32% was observed at the local isolate (HearNPV-IND-K).

Conclusions
It’s concluded that the local isolate had the potential for the development of species-specific and environmentally safe biocontrol agent for organic farming and its incorporation into Integrated Pest Management program may reduce the use of chemical insecticides in Kashmir.
Mondor’s disease of the breast in Asia: a forgotten diagnosis for the front-line clinicians—a case report and literature review
Authors: Vijayan Pavitra , Pang Fui Tin 
Published: April 2023

Abstract
Background
Mondor’s disease is a rare, however, benign and self-limiting condition which can occur in the breast. Diagnosis of Mondor’s disease of the breast is not frequently made as this may be due the lack of awareness on this condition. Although majority of the documented cases were described to be idiopathic, it is wise to properly investigate for other causes such as hypercoagulability or an underlying malignancy. Only nine available case reports pertaining Mondor’s disease of the breast in Asia since 2011 were identified and reviewed.

Main body of abstract
We present a case of a 47-year-old, Malaysian, Malay, female who presented with a right breast swelling which was referred to the Radiology Department to rule out breast abscess. However, further clinical examination and radiological assessment proved it to be superficial thrombophlebitis of the lateral thoracic vein along the upper outer quadrant of her right breast. This condition was attributed to hypercoagulable state secondary to her newly diagnosed diabetes mellitus. After strict diabetic control, short course of antibiotics and symptomatic treatment, complete resolution of signs and symptoms were noted during her follow-up 6 weeks later.

Short conclusion
Mondor’s disease of the breast is believed to be more common than reported as many patients and even clinicians may not pay close attention to this complaint due to the mild symptoms of pain and its self-limiting nature. Raising awareness of the disease through this case review will broaden the scope of differential diagnosis for front-line clinicians when approaching patients presenting with a breast lump.