bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drainage models: An evaluation of their applicability for the design of drainage systems in arid regions
Authors: Ellafi Murad , Simmons Robert , Deeks Lynda 
Published: July 2023

AbstractOnly 5%–10% of irrigated lands in least developed countries (LDCs) are currently drained. Although drainage simulation models (DSMs) are used to evaluate alternative designs, it is unclear which drainage model is suitable for LDCs' arid and semi‐arid regions. This study evaluates selected DSMs (ADAPT, RZWQM2, DRAINMOD, EPIC, HYDRUS‐1D, WaSim and SWAP) and critically assesses their applicability to arid and semi‐arid areas. Also, establish and apply selection criteria based on the availability of data in LDCs with Libya as a case study, and identify the most suitable model for application in Libya. DRAINMOD had the highest overall score, and alternative methods to predict missing input parameters for DRAINMOD are discussed. Evaluating the feasibility of using predicted input parameters for DSMs to design drainage systems in LDCs would help farmers, planners and decision‐makers to reduce the overall cost of drainage system and, also, make DRAINMOD a more accessible tool to evaluate different drainage designs.
Draft genomes of 17 bacterial isolates from Louisiana raw sugarcane factory juices and biofilms
Authors: Qi Yunci , Bruni Gillian 
Published: July 2023

ABSTRACT

We report the draft genomes for 17 bacterial isolates belonging to the genera
Gluconobacter
,
Leuconostoc
, and
Pantoea
that were obtained from Louisiana raw sugarcane factory juice and biofilm samples.
Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
Authors: Lv Linxi , Jiang Hui , Song Dianze , Zhou Xiaoqin , Chen Feng , Ren Long , Xie Yongen , Zeng Mei 
Published: July 2023

Abstract
Background
Previous studies have revealed that Sirt3 deficiency is associated with several inflammatory responses. The purpose of this study is to investigate the role and potential molecular mechanisms of Sirt3 in the inflammation induced by monosodium urate (MSU) crystals.

Methods
The Sirt3 expression level in the peripheral blood mononuclear cells (PBMCs) of patients with gout was measured. Function and molecular mechanism of Sirt3 in MSU crystal-induced inflammation were investigated in bone marrow-derived macrophages (BMDMs), C57BL/6 mouse, and Sirt3−/− mouse.

Results
Sirt3 expression was decreased in the PBMCs of patients with gout. Sirt3 agonist (Viniferin) inhibited the acetylation levels of mitochondrial proteins including the SOD2 protein. RNA sequencing, bio-informatics analysis, RT-PCR, and Western blot demonstrated that Sirt3 could suppress the expression of Acod1 (Irg1), which plays an important role in gout. In BMDMs treated with palmitic acid (C16:0) plus MSU crystals, Acod1 knockdown repressed mitochondrial reactive oxygen species (mtROS) over-production, macrophage migration, and mitochondrial fragmentation, and Acod1 improved AMPK activity. The over-expression of Acod1 did not significantly affect the level of itaconic acid, but greatly decreased the levels of some important intermediate metabolites of the tricarboxylic acid (TCA) cycle. These data indicate that Acod1 exerts a pro-inflammatory role in MSU crystal-induced inflammation and is independent of the metabolic level of itaconic acid. Sirt3 deficiency exacerbates inflammatory response induced by MSU crystals in vitro and in vivo.

Conclusion
The current study has shown that Sirt3 can alleviate the MSU crystal-induced inflammation by inhibiting the expression of Acod1.
Screening the anthelmintic effects of leaves and fruits extracts from various Acacia species as a potential feed for small-ruminant animals
Authors: HANDAYANTA EKA , HADI RENDI FATHONI 
Published: July 2023

Abstract. Handayanta E, Hadi RF. 2023. Screening the anthelmintic effects of leaves and fruits extracts from various Acacia species as a potential feed for small-ruminant animals. Biodiversitas 24: 3140-3144. This study aimed to determine the anthelmintic properties of leaves and fruits obtained from various Acaciaspecies against Haemonchus contortus Rudolphi, 1803. Both leaf and fruit samples were allocated into five different groups: negative control (NC), 0.9% of NaCl solution; positive control (PC), albendazole solution; and solutions containing 12% concentration of each Acacia auriculiformis A.Cunn. ex Benth.(AA), Acacia mangium Willd.(AM), and Acacia crassicarpa A.Cunn. ex Benth.(AC) powder. The added concentration was set at 12%, and each treatment group was brought to incubation in 6 different time parameters (1, 2, 3, 4, 5, and 6 hours) against H. contortus. Each Acacia leaves extract did not significantly differ in eliminating H. contortus, unless for AM under 4 hours of incubation (p<0.05), wherein at the final incubation time, all Acacia-treated samples displayed complete mortality. Meanwhile, treatment with Acacia fruits during 6-hour incubations led to a removal of 76.66-83.33 % H. contortus population, in which Albendazole-treated groups showed complete mortality. Thus, this study suggested the leaves from Acacia are a potential animal feed as anthelmintic against H. contortus parasites. However, further studies investigating the potential mechanism by which various Acacia species present anthelmintic activity are necessary.
Participation of Secondary School Learners Aged 14 to 16 Years in Organised Sport
Authors: Mia Mohamed Ayyub , Malema Makhaya , Leach Lloyd 
Published: July 2023

Background: In South Africa, a relatively small number of children and adolescents belong to sports clubs, and adolescent participation in organised sport has steadily declined, especially in recent years. Participation levels in organised sport has decreased during late childhood and, more increasingly, as adolescents grow older.
Aim: Therefore, the aim of this study was to determine the participation of secondary school learners aged 14 to 16 years in organised sport.
Methods: The study was a quantitative and descriptive, cross-sectional design. The study sample was 329 conveniently sampled secondary school learners, males, and females, aged 14 to 16 years from three state schools in Cape Town, Western Cape Province. A researcher-generated, self-administered questionnaire on organised sport participation was developed, piloted, and validated among 44 learners of similar background, who did not participate in the main study. The statistical software, SPSS version 27, was used to capture and analyse the research data. The level of statistical significance for the study was set at p &lt; 0.05.
Results: A total of 66.9% of participants in the study sample participated in organised sport. Low but significant correlations were reported between gender and participation in organised sport (r = 0.22; p &lt; 0.001), gender and friend participation in organised sport (r = 0.24; p &lt; 0.001), participants participation and sibling participation in organised sport (r = 0.23; p &lt; 0.001), participants participation and friend participation in organised sport (r = 0.36; p &lt; 0.001), and between participant participation and parental participation in organised sport (r = 0.17; p = 0.003).
Conclusion: Adolescent participation in organised sport was lower in the present circumstances compared to participation in the past three years. Adolescent males had higher participation levels in organised sport compared to adolescent females. There is an urgent need for solutions to counteract the low levels of adolescent participation in organised sport, especially in adolescent females.
Functional study of Amorpha fruticosa WRKY20 gene in response to drought stress
Authors: Li Danni , Gu Baoxiang , Huang Chunxi , Shen Jiayi , Wang Xin , Guo Jianan , Yu Ruiqiang , Mou Sirui , Guan Qingjie 
Published: July 2023

The WRKY gene family in plants regulates the plant&#039;s response to drought through regulatory networks and hormone signaling. AfWRKY20 (MT859405) was cloned from A. fruticosa (Amorpha fruticosa,A. fruticosa) seedlings using RT-PCR. The binding properties of the AfWRKY20 protein and the W-box (a DNA cis-acting element) were verified both in vivo and in vitro using EMSA and Dual-Luciferase activity assays. RT-qPCR detected that the total expression level of AfWRKY20 in leaves and roots was 22 times higher in the 30% PEG6000 simulated drought treatment compared to the untreated group. Under the simulated drought stress treatments of sorbitol and ABA (abscisic acid,ABA), the transgenic tobacco with the AfWRKY20 gene showed enhanced drought resistance at the germination stage, with significantly increased germination rate, green leaf rate, fresh weight, and root length compared to the WT (wild type,WT) tobacco. In addition, the SOD (superoxide dismutase,SOD)activity, chlorophyll content, and Fv/Fm ratio of AfWRKY20 transgenic tobacco were significantly higher than those of the WT tobacco under natural drought stress, while the MDA (malondialdehyde,MDA) content and DAB (3,3&#039;-diaminobenzidine,DAB) and NBT (nitroblue tetrazolium,NBT) staining levels were lower. The expression levels of oxidation kinase genes (NbSOD, NbPOD, and NbCAT) in transgenic tobacco under drought stress were significantly higher than those in WT tobacco. This enhancement in gene expression improved the ability of transgenic tobacco to detoxify ROS (reactive oxygen species,ROS). The survival rate of transgenic tobacco after natural drought rehydration was four times higher than that of WT tobacco. In summary, this study revealed the regulatory mechanism of AfWRKY20 in response to drought stress-induced ABA signaling, particularly in relation to the ROS. This finding provides a theoretical basis for understanding the pathways of WRKY20 involved in drought stress, and offers genetic resources for molecular plant breeding aimed at enhancing drought resistance.
Unveiling Strong Dependence of Geometrical Aspect Ratio on the Magneto-Structural Properties of Co2Mn-Based Microwires
Authors: Wederni Asma , Salaheldeen Mohamed , Ipatov Mihail , Zhukova Valentina , Zhukov Arkadi 
Published: July 2023

The present study illustrates the strong effect of geometrical parameters on the magneto-structural properties of Co2MnSi glass-coated microwires prepared using the Taylor-Ulitovsky method. Thus, two samples with different geometrical aspect ratios (ρ= d / D) have been fabricated, where d is the metallic nuclei diameter and D is the total diameter of the Co2MnSi microwire. The XRD analysis shows a significant change by modifying the aspect ratio, for ρ = 0.46 the main peak with miller indices (220) is recognized as an A2-type disordered cubic structure. Meanwhile, in the sample with a low aspect ratio, ρ = 0.31, the perfect L21 ordered cubic structure is attained. In addition, a decrease in average grain size from 36.62 nm to 28.07 nm by increasing the aspect ratio from 0.46 to 0.31 is observed. The magnetic characterization has been done in a wide range of temperatures and magnetic fields. Both of Co2MnSi samples show ferromagnetic behavior in a whole measuring temperatures range. A significant increase in coercivity and normalized reduced remanence by decreasing the aspect ratio is observed. The change in the magnetic properties is attributed to the modification in the microstructure, which is induced during the fabrication process. Such a dependence on the microstructure and magnetic properties on the ρ-ratio can be associated either with the internal stresses distribution and magnitude or with different quenching rates of microwires with different aspect ratios. The current findings demonstrate the tunability of microstructure and magnetic properties of Co2MnSi-glass-coated microwires simply, by a small modification in the geometric properties during the manufacturing process and without excreting any additional post-processing. The variation in geometric parameters of Co2MnSi glass-coated microwires allows us to tune magnetic properties and structure, which is essentially advantageous for sensing devices development.
Risk Factors for All-Cause Early Reoperation Following Tumor Resection and Endoprosthetic Reconstruction
Authors: Kendal Joseph K. , Slawaska-Eng David , Gazendam Aaron , Schneider Patricia , Wessel Lauren E. , Ghert Michelle , Bernthal Nicholas M. ,   on behalf of the PARITY Investigators
Published: July 2023

Background:
Oncologic resection and endoprosthetic reconstruction of lower-extremity musculoskeletal tumors are complex procedures fraught with multiple modes of failure. A robust assessment of factors contributing to early reoperation in this population has not been performed in a large prospective cohort. The aim of the present study was to assess risk factors for early reoperation in patients who underwent tumor excision and endoprosthetic reconstruction, with use of data from the Prophylactic Antibiotic Regimens in Tumor Surgery (PARITY) trial.

Methods:
Baseline characteristics were assessed, including age, sex, tumor type, tumor location, presence of a soft-tissue mass, diabetes, smoking status, chemotherapy use, and neutropenia. Operative factors were recorded, including operative time, topical antibiotics, silver-coated prosthetics, endoprosthetic fixation, extra-articular resection, length of bone resected, margins, tranexamic acid, postoperative antibiotics, negative-pressure wound therapy, and length of stay. Univariate analysis was utilized to explore the differences between patients who did and did not undergo reoperation within 1 year postoperatively, and a multivariate Cox proportional hazards regression model was utilized to explore the predictors of reoperation within 1 year.

Results:
A total of 155 (25.7%) of 604 patients underwent ≥1 reoperation. In univariate analysis, tumor type (p < 0.001), presence of a soft-tissue mass (p = 0.045), operative time (p < 0.001), use of negative-pressure wound therapy (p = 0.010), and hospital length of stay (p < 0.001) were all significantly associated with reoperation. On multivariate assessment, tumor type (benign aggressive bone tumor versus primary bone malignancy; hazard ratio [HR], 0.15; 95% confidence interval [CI], 0.04 to 0.63; p = 0.01), operative time (HR per hour, 1.15; 95% CI, 1.10 to 1.23; p < 0.001), and use of negative-pressure wound therapy (HR, 1.93; 95% CI, 1.30 to 2.90; p = 0.002) remained significant predictors of reoperation within 1 year.

Conclusions:
Independent variables associated with reoperation within 1 year in patients who underwent tumor resection and endoprosthetic reconstruction included tumor type (benign aggressive bone tumor versus primary bone malignancy), operative time, and use of negative-pressure wound therapy. These results will help to inform patients and surgeons regarding the risk of reoperation by diagnosis and reinforce operative time as a factor influencing reoperation. These results also support further investigation into the use of negative-pressure wound therapy at the time of surgery in this patient population.

Level of Evidence:
Therapeutic Level II. See Instructions for Authors for a complete description of levels of evidence.
Pediatric and Adult Patients Have Similar Functional Improvement After Endoprosthetic Reconstruction of Lower-Extremity Tumors
Authors: Tran Tina H. , Hayden James B. , Gazendam Aaron M. , Ghert Michelle , Gundle Kenneth R. , Doung Yee-Cheen ,   on behalf of the PARITY Investigators
Published: July 2023

Background:
Although the treatment of lower-extremity bone tumors is similar between adult and pediatric patients, differences in outcomes are unknown. Outcomes for lower-extremity oncologic reconstruction have been challenging to study because of the low incidence and heterogeneity in disease and patient characteristics. The PARITY (Prophylactic Antibiotic Regimens in Tumor Surgery) trial is the largest prospective data set assembled to date for patients with lower-extremity bone tumors and presents an opportunity to investigate differences in outcomes between these groups.

Methods:
Patient details were acquired from the prospectively collected PARITY trial database. The 1993 Musculoskeletal Tumor Society (MSTS-93) and Toronto Extremity Salvage Score (TESS) questionnaires were administered preoperatively and at 3, 6, and 12 months postoperatively. Continuous outcomes were compared between groups with use of the Student t test, and dichotomous outcomes were compared with use of the Pearson chi-square test.

Results:
A total of 150 pediatric and 447 adult patients were included. Pediatric patients were more likely than adult patients to have a primary bone tumor (146 of 150 compared with 287 of 447, respectively; p < 0.001) and to have received adjuvant chemotherapy (140 of 149 compared with 195 of 441, respectively; p < 0.001). Reoperation rates were not significantly different between age groups (45 of 105 pediatric patients compared with 106 of 341 adult patients; p ≤ 0.13). Pediatric patients had higher mean MSTS-93 scores (64.7 compared with 53.8 among adult patients; p < 0.001) and TESS (73.4 compared with 60.4 among adult patients; p < 0.001) at baseline, which continued to 1 year postoperatively (mean MSTS-93 score, 82.0 compared with 76.8 among adult patients; p = 0.02; mean TESS, 87.7 compared with 78.6 among adult patients; p < 0.001). Despite the differences in outcomes between cohorts, pediatric and adult patients demonstrated similar improvement in MSTS-93 scores (mean difference, 17.4 and 20.0, respectively; p = 0.48) and TESS (mean difference, 14.1 and 14.7, respectively; p = 0.83) from baseline to 1 year postoperatively.

Conclusions:
Pediatric patients had significantly better functional outcomes than adult patients at nearly all of the included postoperative time points; however, pediatric and adult patients showed similar mean improvement in these outcomes at 1 year postoperatively. These findings may be utilized to help guide the postoperative expectations of patients undergoing oncologic reconstruction.

Level of Evidence:
Prognostic Level II. See Instructions for Authors for a complete description of levels of evidence.
Assessing Digital Learning Platforms in Higher Education with Reference to Student Satisfaction- A PLS-SEM Approach
Authors: Singh Harendra , Singh Vikrant Vikram , Gupta Aditya Kumar , Kapur P K 
Published: July 2023

Abstract

Knowledge and information are crucial to any society's progress; hence the level of education and awareness of its citizens directly affects that progress. In this economy, various factors come into play, such as the availability of educated and skilled workers, the development of novel and efficient systems, and the state of the information infrastructure. The recent epidemic, which prompted widespread lockdowns and restrictions, will continue to be felt for decades. The repercussions of such events have been most noticeable among educators and their students. Thus online education is need of the hour to make sure quality education reaches to each corner of the world on timely basis. So the assessment of digital learning platform in higher education institutions of India is the primary topic of this research. This study examines the various facets of student satisfaction as well as the future of online learning. 460 responses were collected via a Likert-scale questionnaire in a randomly selected online survey. The R programming language was used to do a partial least squares structural equation modeling analysis on the data in R studio. Based on the findings of the study, it is clear that both students and teachers will be able to adapt throughout the course of time, and it is possible that the teaching-learning process will evolve into a hybrid form of education.
Chemical Composition and antimicrobial activity of essential oils of two wild olive subspecies Olea europaea subsp. europaea var. Sylvestris and the endemic Olive Olea europaea subsp. lapperinie
Authors: Bouchoucha Sara , BOUKHEBTI Habiba , Oulmi Abdemalek , Mouhamadi Yacine , Chaker Adel Nadjib 
Published: July 2023

Abstract

Two wild olive subspecies fixed in this research: Olea europaea subsp. europaea var. sylvestrisand Olea europaea subsp. laperrinei despite its ecological value, the chemical composition of subsp. laperrinei oil remains unknown, the samples were harvested from the different geographical area. Gas Chromatography-Mass Spectrometry(GC-MS) and Gas Chromatography-flame-ionization detection (GC-FID) analysis of subsp europaea var. sylvestris allowed the identification of 29 compounds oil with Nonanal (11.82%), theaspiranea A (9.81%), 3-hexen-1-ol,benzoate(9.31%) as a major constituents , while the subspecies of the Saharan region were resultedthe identification of 31 compounds where α-pinene (16%) , β-Ocimene (12.82%), dl-Limonene(8.20%) was the main components . The results of the disc diffusion method showed that the two volatile oils have efficient antibacterial activity but, subsp. laperrinei essential oil has a higher range of inhibition, in which P. aeruginosa and B. subtilis showed extreme sensitivity, while the K. pneumoniae bacterium shows great resistance to the two essential oils.
Effects of the Exposure of Aged Micro-Polyethylene Terephthalate on the Growth Status and Photosynthesis of Chlorella sp. UTEX1602
Authors: Li Ruikang , Song Chunfeng 
Published: July 2023

Abstract
Microplastics (MPs) are ubiquitous in aquatic ecosystems worldwide, but their effects on plankton, especially the effect of aged MPs on freshwater microalgae, are not well-known. To investigate this issue, we studied the effects of untreated micro-polyethylene terephthalate (untreated-mPET) and strong alkali micro-polyethylene terephthalate (NaOH-mPET), strong acid micro-polyethylene terephthalate (HCl-mPET), high temperature micro-polyethylene terephthalate (HT-mPET) and ultraviolet micro-polyethylene terephthalate (UV-mPET) on the growth and photosynthetic pigment yield of Chlorella sp. UTEX1602 (8d). The results showed that aged mPET enhanced the toxicity of mPET to Chlorella sp. UTEX1602 compared to the toxicity of untreated-mPET. Moreover, 100 mg/L and 200 mg/L NaOH-mPET, and 20 mg/L UV-mPET and HT-mPET inhibited the growth of Chlorella sp. UTEX1602, as well as chlorophyll and carotenoids. The changes in the concentration of key active enzymes also confirmed the toxic effect of MPs on Chlorella sp. UTEX1602. Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS) and Zetasizer were used to determine the effect of aging on the surface characteristics of mPET. The reduction of hydroxyl and zeta potential affected the interaction between MPs and microalgae, thereby increasing the toxicity of aged MPs. By using a liquid chromatography-high-resolution quadrupole time-of-flight tandem mass spectrometer, we determined the effect of aging on the leachate of MPs. The increased content of dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) indicated that the toxic effect of MPs on microalgae might be caused by MPs and the leachate of MPs. Aged-mPET stimulated an increase in the content of extracellular polysaccharides (EPS) produced by Chlorella sp. UTEX1602 and alduronic acid in EPS; thus, the biopolymer based on microalgae can be used as a bio-flocculant to remove MPs. The results help to better understand the impact of aging on MPs in the environment and presented here provide more evidence regarding the risks that MPs bring into freshwater ecosystems. Future studies on MPs aging should narrow the knowledge gap between laboratory simulations and actual conditions and increase environmental relevance.