bluuebunny/crossref_metadata_2025_split huggingface dataset:
Minimally invasive percutaneous "three point &amp; two step" procedure: a novel operation for type II Galeazzi fracture without severing pronator quadratus
Authors: Dong Liu , Xiao Wang , Yu Zhang , Bo Jiang , Lumin Cao , Jun Hua 
Published: January 2023

Abstract
Purpose: To explore the clinical efficacy of minimally invasive percutaneous internal fixation "three point & two step" procedure for type II Galeazzi fracture without severing pronator quadratus.
Methods: Patients admitted to the orthopedic department between December 2018 and February 2022 owing to type II Galeazzi fracture were eligible for analysis.All participants underwent "three point - two step", minimally invasive percutaneous, plate internal fixation, and were assessed for the surgical duration, intraoperative blood loss, postoperative fracture healing, and functional recovery of the affected limb.
Results: The follow-up duration was 20.5 ± 10.7 months; the distal incision was 27.5 ± 2.5 mm and the proximal incision was 35 ± 3.78 mm; overall 38.21 ± 30.27 ml of intraoperative blood loss occurred among the analyzed patients; otherwise, all patients showed good alignment of the lower ulnar and radial joints, with no fracture nonunion. The patients received DASH and Mayo scores of 6.37 ± 1.24 and 88.21 ± 8.99, respectively, at 6 months post operation, and exhibited an excellent rate of 85.7%.
Conclusions: The minimally invasive percutaneous internal "three point & two step" fixation for type II Galeazzi fracture has the advantages of minimal trauma, rapid recovery, and maximal regaining of wrist function. Thus, this procedure should be clinically promoted.
A potential role for inflammatory cytokines in a rare late-onset capsular block syndrome: a case report
Authors: Du Ying-Hua , Liang Xiao-Fang , Hirooka Kazuyuki , Xia Hui-Ka , Jia Zhi-Yang 
Published: January 2023

Abstract
Background: late-onset capsule block syndrome (CBS) was a rare complication of cataract surgery and its pathogenesis was unclear.
Case presentation: An analysis of a 64-year-old female with late-onset CBS was performed retrospectively. The depth of the anterior chamber (ACD), the distance between the face of the retro-implanted intraocular lens (IOL) and the posterior capsule (DRP), the best-corrected visual acuity (BCVA), and the visual quality (VQ) were all measured both before and after surgery. Using a flow cytometer multiple array assay device, we assessed the levels of inflammatory cytokines in the opaque substances (OS) that were trapped between the posterior capsule and the retro-IOL surface and compared them to those in aqueous humor. The patient who underwent surgery experienced a considerable improvement in BCVA and VQ. DRP was on the verge of disappearing. However, ACD did not differ between pre-and post-operatively. Interleukin-8 (IL-8) and basic fibroblast growth factor (BFGF) concentrations were higher in the OS than in aqueous humor, especially in the former. Vascular cell adhesion molecule (VCAM) concentration in the OS, however, was lower than in aqueous humor.
Conclusions: Surgery was a successful treatment for a challenging late-onset CBS. A fresh, isolated microenvironment developed between the posterior capsule and the retro-IOL face. In the human lens, inflammatory cytokines from the OS might contribute to abnormal metabolism in the sealed area as a result of late-onset CBS.
Thermal Behaviour of Agro-waste Based Ceiling Board and Its Filler Material
Authors: Ezenwa Obiora Nnaemeka , Obika Echezona Nnaemeka , Ekengwu Ignatius Echezona , Okafor Obiora 
Published: January 2023

Abstract
The thermal behavior of a ceiling board produced with breadfruit seed coat as the filler material, and recycled Low Density Polyethylene, as the binder, was investigated. The Breadfruit seed coat was treated with sodium hydroxide and acetic acid, to remove the pigments. The ceiling material was produced with 19.722% filer-binder ratio, and pressed for 10 minutes, at temperature and pressure of 197.31 oC and 9.042 MPa respectively. Lee-Charton’s apparatus was used to determine the thermal conductivity of the produced material, which gave a value of 0.362 Wm− 1K− 1 and the corresponding thermal diffusivity and resistivity of 5.24 x 10− 7 m2/s, and 2.76 Wm− 1K− 1, respectively. Thermo-gravimetric analyzer and Digital Scanning Calorimeter was used to study the thermal behavior the filler material and the produced sample, which showed a good thermal stability. Hence, this research showed that the produced material is suitable for ceiling board application.
Pediatric autoimmune diseases with ELANE gene mutation associated with neutropenia
Authors: Zhang Dan , Su Gaixiu , Hao Sheng , Lai Jianming , Feng Shunqiao 
Published: January 2023

Abstract
Objective

To explore the clinical characteristics of autoimmune diseases in children with ELANE gene mutation.
Methods: Three cases of children with ELANE gene mutation manifested as autoimmune disease were retrospectively analyzed from April 2020 to May 2021.
Results: They were 2 males and 1 female, aged 15 years, 1 year and 10 months and 1 year and 10 months, respectively. All of them had recurrent infection. Case 1 presented with cyclic neutropenia and systemic lupus erythematosus. Case 2 presented with severe neutropenia and autoimmune hemolytic anemia. Case 3 presented with severe neutropenia and ANCA-associated small vasculitis. Genetic tests showed that they all had heterozygous mutations in ELANE gene. Case 1 was treated with methylprednisolone and hydroxychloroquine sulfate for 2 years, and had normal neutrophils. Case 2 received allogeneic hematopoietic stem cell transplantation and has stopped taking all drugs. Case 3 received subcutaneous injection of granulocyte colony-stimulating factor, oral prednisone and cyclophosphamide. He has been followed up for one year, and his neutrophils increased to 1.56×109/L.
Conclusion Patients with ELANE gene mutation may have recurrent infection, and combined with autoimmune disease. DMARDs are effective for autoimmune disease. Allogeneic hematopoietic stem cell transplantation can cure the disease.
Postharvest starch and sugars adjustment in potato tubers of wide-ranging dormancy genotypes subjected to various sprout forcing techniques
Authors: Haider Muhammad Wasim , Nafees Muhammad , Iqbal Rashid , Asad Habat Ullah , Azeem Farrukh , Shaheen Ghazala , Iqbal Javed , Vyas Shweta , Arslan Muhammad , Rahman Muhammad Habib Ur 
Published: January 2023

Abstract
Development of an efficient, safe and environment-friendly technique to terminate tuber dormancy in potatoes (Solanum tuberosum L.) is of great concern due to an immense scope of multiple cropping all over the globe. The breakage of tuber dormancy has been associated with numerous physiological changes including a decline in the level of starch and increase in the levels of sugars during storage of freshly harvested seed potatoes, although their consistency across the genotypes and various dormancy breaking techniques have not yet been fully elucidated. The purpose of the present research is to assess the efficacy of four different dormancy breaking techniques such as soaking in 90, 60, or 30 mgL−1 solutions of benzylaminopurine (BAP) and 30, 20, or 10 mgL−1 gibberellic acid (GA3) alone and in the combination of optimized concentrations; cold pre-treatment at 6, 4, or 2°C; electric shock at 80, 60, 40, or 20 Vs; and irradiation at 3.5, 3, 2.5, 2, 1.5, or 1 kGy on tuber dormancy period and sprout length of six genotypes. Furthermore, the changes occurred in tuber weight and endogenous starch, sucrose, fructose, and glucose contents in experimental genotypes following application of these techniques were also examined. Overall, the most effective technique to terminate tuber dormancy and hasten spout growth was the combined application of BAP and GA3, which reduced the length of dormancy by 9.6 days compared to the untreated control, following 6.7 days of electric current, 4.4 days of cold pre-treatment, and finally irradiation (3.3 days). The 60 mgL−1 solution of BAP greatly reduced the dormancy period in all genotypes but did not affect the sprout length at all. The genotypes showed a negative weak correlation (r ≤ − 0.4) of endogenous starch contents with dormancy breakage and weight loss or moderate (r ≤ − 0.5) correlation with sprout length; but a strong positive correlation (r ≥ 0.8) of tuber glucose, fructose, and sucrose contents with dormancy breakage and weight loss. During three weeks storage, sprouting commencement and significant weight loss occurred as tuber dormancy advanced towards breakage due to reduction in starch and increase in sucrose, fructose, and glucose contents of tubers. These findings could be advantageous for postponing or accelerating seed potato storage as well as investigating related physiological research in the future.
Differences between persons with and without disability in HIV prevalence, testing, treatment, and care cascade in Tanzania: A cross-sectional study using population-based data
Authors: Chipanta David , Mitra Sophie , Amo-Agyei Silas , Velarde Minerva Rivas , Amekudzi Kofi , Osborne Connie , Estill Janne , Keiser Olivia 
Published: January 2023

Abstract
Background
Persons with disability may have a higher HIV prevalence and be less likely than persons without disability to know their HIV-positive status, access antiretroviral therapy (ART), and suppress their HIV viral load (HIV testing, treatment, and care cascade, also known as the 90—90—90 and the 95—95—95 targets set to be achieved by 2020 and 2025, respectively).
Methods
Using the Tanzania HIV Impact Survey (THIS) data collected between October 2016 and August 2017, we assessed differences in HIV prevalence and progress towards achieving the 2020 HIV testing, treatment, and care cascade target between persons with and without disability. We presented the sample characteristics by disability status and analyzed the achievement of the cascade target by disability status, and sex. We used multivariate logistic regressions, and adjusted for age, sex, rural-urban-residence, education, wealth, and region.
Results
The sample comprised 1,831 people living with HIV (PLHIV) with a median age of 32 years (IQR 21—45). Approximately 11.8% (95% Confidence Interval [CI]: 10.2%—13.6%, n= 212) of PLHIV in Tanzania were disabled. HIV prevalence was higher (6.2%, 95% CI: 5.3%—7.4%) among persons with disability than persons without disability (4.7%, 95% CI: 4.4% — 5.1%); Women with disability were more aware of their HIV-positive status (n = 101, 79.0%, 95% CI: 68.0%—87.0% versus n = 703, 63.0%, 95% CI: 59.1%—66.7%) and accessed ART more frequently (n = 98, 98.7%, 95% CI: 95.3%—99.7% versus n = 661, 94.7%, 95% CI: 92.6%—96.3%) than women without disability. After adjusting for socio-demographic characteristics, the odds of having HIV and of accessing ART were not statistically different between PLHIV with and without disability. However, PLHIV with disability had higher odds of being aware of their HIV-positive status (aOR 1.69, 95% 1.05—2.71) than PLHIV without disability. Men on ART with disability had lower odds (aOR = 0.23, 95% CI: 0.06—0.86) to suppress HIV viral loads than their counterparts without disability.
Conclusion
We found no differences in HIV prevalence and access to ART between persons with and without disability in Tanzania. Whereas PLHIV disability, women in particular, were advantaged in knowing their HIV-positive status, men on ART with disability may have been disadvantaged in having suppressed HIV viral loads. These differences are correctable by disability-inclusive HIV programming. HIV surveys in multiple countries sampling more persons with disability are required to measure differences in HIV prevalence better and in attaining the 2025 HIV testing and treatment cascade target for both persons with and without disability.
Establishment and Verification of Prognostic Model and ceRNA Network Analysis for Colorectal Cancer Liver Metastasis
Authors: Zhang Xuan , Wu Tao , Zhou Jinmei , Chen Xiaoqiong , Dong Chao , Guo Zhangyou , Yang Renfang , Liang Rui , Feng Qing , Hu Ruixi , Li Yunfeng , Ding Rong 
Published: January 2023

Abstract
Objects:
Colorectal cancer (CRC) is one of the most common cancers in the world. Approximately two-thirds of patients with CRC will develop colorectal cancer liver metastases (CRLM) at some point in time. In this study, we aimed to construct a prognostic model of CRLM and its competing endogenous RNA (ceRNA) network.
Methods
RNA-seq of CRC, CRLM and normal samples were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. Limma was used to obtain differential expression genes (DEGs) between CRLM and CRC from sequencing data and GSE22834, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were performed, respectively. Univariate Cox regression analysis and lasso Cox regression models were performed to screen prognostic gene features and construct prognostic models. Functional enrichment, estimation of stromal and immune cells in malignant tumor tissues using expression data (ESTIMATE) algorithm, single-sample gene set enrichment analysis (ssGSEA), and ceRNA network construction were applied to explore potential mechanisms.
Results
An 8-gene prognostic model was constructed by screening 112 DEGs from TCGA and GSE22834. CRC patients in the TCGA and GSE29621 cohorts were stratified into either a high-risk group or a low-risk group. Patients with CRC in the high-risk group had a significantly poorer prognosis compared to in the low-risk group. The risk score was identified as an independent predictor of prognosis. Functional analysis revealed that the risk score was closly correlated with various immune cells and immune-related signaling pathways. And a prognostic gene-associated ceRNA network was constructed that obtained 3 prognosis gene, 14 microRNAs (miRNAs) and 7 long noncoding RNAs (lncRNAs).
Conclusions
In conclusion, a prognostic model of CRLM proposed that independently identify high-risk patients with low survival, suggest a relationship between local immune status and prognosis, and construct a ceRNA network. Our study may provide inspiration for further research on the pathogenesis of CRLM.
Disparities and Determinants of Maternal Health Services Utilization Among Women in Poverty-Stricken Rural Areas of China: A Cross-sectional Study
Authors: Yang Yuxuan , Yu Min 
Published: January 2023

Abstract
Background:Reducing maternal mortality rate (MMR) has been a worldwide public health challenge for a long time. Utilization of maternal health services including antenatal care (ANC), institutional delivery (ID), and postnatal care (PNC) is vital to prevent maternal mortality. China has made significant improvements in maternal health during the past 30 years, however, disparities in maternal health service utilization still exist among regions and the western rural areas had the lowest utilization rate. This study aims to assess the inequality and determinants of maternal health service utilization in western poverty-stricken rural areas based on Anderson’s Behavioral Model of Health Service Use and provide evidence-based suggestions to improve equity and coverage of maternal service utilization in China.
Methods:A cross-sectional study was conducted in Gansu and Yunnan Province, Western China using primary data (n=996) collected by the research team. A multistage, judgment, quota sampling procedure was employed to select the participants of the survey. Trained local health staff formed an interview team to help respondents answer a structured, pre-tested questionnaire designed based on Anderson’s model. Data collected through interviews were used for descriptive analysis, and univariate and multivariate binary logistic analysis to identify influencing factors of 8+ANC, ID, and 2+PNC utilization.
Results: Place of residence, education level, per capita household income, conditional cash transfer (CCT) participation, and distance to health facilities were influencing factors of 8+ANC; place of residence, education level, and availability of financial incentive programs were influencing factors of ID; number of children, health education during ANC, CCT projects participation, and self-rated health status were influencing factors of 2+PNC.
Conclusions:Inequalities in maternal service utilization exist between Yunnan and Gansu provinces. This study shows a strong association between both predisposing and enabling factors and maternal services utilization. Predisposing factors such as place of residence, education level, and number of children, enabling factors such as CCT participation, annual income, health education during ANC, and distance to health facilities along with need factor self-rated health status all contribute to maternal services utilization.
We conclude that many factors influence maternal service utilization and interventions targeted at various levels should be considered. Therefore, we suggest more health resources should be invested in underutilized areas, financial incentive projects targeting pregnant women should be implemented, and health education should be provided to improve women’s health literacy.
Machine learning-based analysis predicts a person's gender based on mechanical, but not thermal, pain thresholds
Authors: Lotsch Jorn , Mayer Benjamin , Kringel Dario 
Published: January 2023

Abstract
Gender differences in pain perception have been extensively studied, while precision medicine applications such as gender-specific pain pharmacology have barely progressed beyond proof-of-concept. A data set comprising pain thresholds to mechanical (blunt and punctate pressure) and thermal (heat and cold) stimuli applied to nonsensitized and sensitized (capsaicin, menthol) forearm skin of 69 male and 56 female healthy volunteers was analyzed for data structures contingent with the prior gender structure, using unsupervised and supervised approaches. A working hypothesis that the relevance of gender differences could be approached via reversibility of the association, i.e., genders should be identifiable from pain thresholds, was verified with trained machine-learning algorithms that could infer a person’s gender in a 20% validation sample not seen to the algorithms during training, with a balanced accuracy of up to 79%. This was only possible with thresholds for mechanical stimuli, but not for thermal stimuli or responses to sensitization, which were not sufficient to train an algorithm that could assign gender better than by guessing or when trained with nonsense (permuted) information. This enabled translation to the molecular level of nociceptive targets that convert mechanical but not thermal information into signals that are interpreted as pain, which could eventually be used for pharmacological precision medicine approaches to pain. By exploiting a key feature of machine learning that enables the recognition of data structures and the reduction of information to the bare minimum relevant, experimental human pain data could be characterized in a way that incorporates "non" logic that could be transferred directly to the molecular pharmacological level, pointing a way toward gender-specific precision medicine for pain.
Oral microbiota dysbiosis and increased inflammatory cytokines with different stroke subtypes
Authors: Jing Zhen , Xie Xiaomei , Gao Jiali , Yang Jiajie , Leng Xinyi , Chen Guobing , Liang Dan , Liu Jialin , Zhang Shijun , Huang Li'an 
Published: January 2023

Abstract
Background: The possible correlation between oral microbiota dysbiosisand acute ischemic stroke, regarding different pathogenesis and stroke severity, remains unclear. Therefore, this study aimed to identify the specific microbiota for different subtypes of stroke to discover the underlying risk factors for ischemic stroke, which is of important clinical research value.
Methods:Oral microbiota communities from 162 stroke patients and 62 stroke-free controls were prospectively assessed by sequencing the V3–V4 region of the 16S DNA gene. Demographic and clinical data were obtained for both groups. Triglycerides, total cholesterol, low-density lipoprotein, homocysteine, high-sensitivity C-reactive protein, SLCO1B1, APOE, CYP2C19, IL6, IL8, IL1β, TNF-α, and sCD40L were measured, and their relationship with oral microbiota was analyzed. Cranial magnetic resonance and carotid artery ultrasound were performed for both groups within seven days of admission.
Results: IL6, IL8, IL1β, TNF-α, and sCD40L were significantly higher in stroke patients than in controls. Although the oral microbiota of the stroke and control groups were similar in diversity and structure, that of the severe stroke (National Institutes of Health Stroke Scale score > 5) and cardioembolic stroke subgroups differed from those of the control group. Linear discriminant analysis effect size analysis showed that Megasphaera, Prevotella_1, Clostridia, Selenomonas_3, Prevotella_6, and Dialister were mainly enriched in the severe stroke subgroup. Prevotella_6, Staphylococcus, Staphylococcaceae, and Peptostreptococcus were significantly enriched in the cardioembolic stroke subgroup. Spearman correlation analysis revealed that IL6, IL8, IL1β, TNF-α, and sCD40L were significantly correlated with Peptostreptococcus, Staphylococcus, Selenomonas, Megasphaera, and other bacteria (p < 0.01; p < 0.05).
Conclusions: The oral microbiota in stroke patients were not significantly different from that in the stroke-free controls. However, certain stroke subgroups, such as the severe or cardioembolic stroke subgroups, exhibited significant oral microbiota dysbiosis, which was associated with elevated inflammatory cytokines.