bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Anti‐Interfering Platelet Counting Technology Utilizing Conventional Impedance and White Blood Cell Differential Channel
Authors: Ye Yi , Cai Qi , Chen Gengwen , Liu Zongjun , Liu Yan , Bian Xiaosen , Yao Donglan , Xu Keqian 
Published: January 2025

ABSTRACTIntroductionAccurate platelet (PLT) counting is crucial for disease diagnosis and treatment, especially under the condition of thrombocytopenia and platelet transfusion. A few PLT counting approaches have been established including impedance and fluorescent methods. The impedance PLT counting (PLT‐I) approach could be interfered by small non‐PLT particles in the blood, such as RBC/WBC fragments, microcytes, bacteria, and cryoglobulins. The fluorescent PLT counting methods provide a more accurate but rather costly option. Thus, it is highly desirable to develop a new economic‐friendly approach with accurate platelet count. In this study, we introduced a novel hybrid PLT counting (PLT‐H) strategy to combine the count of smaller‐sized PLTs from impedance channel and larger‐sized PLTs from conventional white blood cell (WBC) channel to realize a low‐cost PLT count with high accuracy.MethodsBlood samples with different PLT levels and no significant interfering factors (confirmed by blood smear) were collected. The PLTs were counted with BC‐6800Plus (PLT‐I) and compared with the PLT counts from the CD41/CD61 immunoplatelet (immunoPLT) reference method using Beckman Coulter FC‐500 flow cytometer. In addition, the morphology and internal structure of PLT treated with hemolytic agents of conventional WBC channel was observed with phase contrast microscopy and electron microscopy. Then the counting accuracy of PLT‐H was assessed by comparing PLT count results between BC‐720 (PLT‐H) and the CD41/CD61 immunoPLT reference method.ResultsBy comparing PLT‐I with immunoPLT, it was found that the impedance PLT counting result will not be interfered by small non‐PLT particles when the size of PLT smaller than 10 fL. For large PLT (> 10 fL), PLT count in conventional WBC channel shows good correlations with immunoPLT. Furthermore, after treated with hemolytic agents the PLT still preserves an intact cellular structure and swells slightly, while RBCs are lysis and disappeared upon hemolytic treatment. Lastly, the novel PLT‐H result exhibits a good correlation with immunoPLT with a high correlation factor (r = 0.9911).ConclusionsThe new PLT counting method PLT‐H achieves high accuracy in blood samples with low‐cost, and it provide a novel strategy of combining traditional methods for high accurate counting in hematology laboratories.
South African single surgeon experience: Comparison of oncological outcomes, robot‐assisted radical prostatectomy versus open perineal radical prostatectomy
Authors: Dlamini Khayalethu C. S. , Coetzee Lance J. , Mathabe Kgomotso 
Published: January 2025

BackgroundStudies comparing oncological outcomes between robot‐assisted radical prostatectomy (RARP) and open radical prostatectomy (ORP) are often limited by bias because of their multi‐institutional and multiple surgeon design. Studies from a single institution and single surgeon are uncommon.ObjectiveTo compare oncological outcomes between RARP and ORP at a single institution by a single surgeon.Design, setting and participantsA retrospective cohort study of 2376 men with localized prostate cancer who underwent either RARP or ORP, from 1995 to 2020 at single institution, by one surgeon was done. The first 100 cases were discounted in both cohorts to account for the learning curve.MeasurementsPositive surgical margin (PSM) and biochemical recurrence (BCR) rates were measured for each cohort.ResultsA total of 1566 men underwent ORP and 810 underwent RARP. BCR rates of 29.2% were found in the ORP group versus 19.5% in the RARP group (p < 0.001). PSM rates of 15.4% were found in the ORP group versus 9.0% in the RARP group (p < 0.001). A multivariate analysis of preoperative prostate specific antigen (PSA) and tumor stage (T) shows no statistically significant association with recurrence when controlled for surgical technique.ConclusionsRARP produces better oncological outcomes when compared to ORP when performed by one experienced surgeon at a single institution.Patient summaryIn this large study of men with prostate cancer still localized to the prostate. We found that better cancer removal and chances of cancer recurrence are reduced by a robot‐assisted prostate removal technique, compared to the traditional open technique.
The Evolving T Cell Receptor Recognition Code: The Rules Are More Like Guidelines
Authors: Gray George I. , Chukwuma P. Chukwunalu , Eldaly Bassant , Perera W. W. J. Gihan , Brambley Chad A. , Rosales Tatiana J. , Baker Brian M. 
Published: January 2025

ABSTRACTαβ T cell receptor (TCR) recognition of peptide–MHC complexes lies at the core of adaptive immunity, balancing specificity and cross‐reactivity to facilitate effective antigen discrimination. Early structural studies established basic frameworks helpful for understanding and contextualizing TCR recognition and features such as peptide specificity and MHC restriction. However, the growing TCR structural database and studies launched from structural work continue to reveal exceptions to common assumptions and simplifications derived from earlier work. Here we explore our evolving understanding of TCR recognition, illustrating how structural and biophysical investigations regularly uncover complex phenomena that push against paradigms and expand our understanding of how TCRs bind to and discriminate between peptide/MHC complexes. We discuss the implications of these findings for basic, translational, and predictive immunology, including the challenges in accounting for the inherent adaptability, flexibility, and occasional biophysical sloppiness that characterize TCR recognition.
Exploring the Effect of Cultural Inspiration Distance and Timing on Designer’ Creativity in Targeted Cultural Creative Design Pattern
Authors: Chen Guodong , Li Zuting , Zhao Qixun , Kong Bei , Gao Yana , Pan Rong 
Published: January 2025

AbstractCultural creative design activity and education involve the application of cultural inspiration. This study examines the impact of cultural inspiration distance and timing on designer’ creativity in Targeted Cultural Creative Design Pattern (TCCDP). Four design novice groups attended the cultural product design experiment with a combination of distance (far or near‐cultural inspiration) and timing (receiving inspiration at the beginning or during design). Each participant produced multiple ideas and one final concept based on a given product theme. The results showed an Interaction Effect between distance and timing on idea quantity, with timing having a greater impact on far‐cultural inspiration (FCI) than near‐cultural inspiration (NCI). Exposure to FCI generated the most ideas at the beginning, while during design, the opposite was observed. Exposure to NCI generated a similar idea quantity in both timing. Distance displayed a Main Effect on concept quality, with exposure to NCI achieving higher quality (novelty; feasibility) final concept than exposure to FCI. It was also found that generating more ideas did not necessarily result in higher quality; a more effective strategy might generate a small number of ideas (about 5), and subsequently deepening and optimising the ideas. These findings are helpful to understand the role of cultural inspiration in cultural creative design education and practice.
Distinguishing Chronic Inflammatory Demyelinating Polyneuropathy From Mimic Disorders: The Role of Statistical Modeling
Authors: Swart Grace , Skolka Michael P. , Shelly Shahar , Lewis Richard A. , Allen Jeffrey A. , Dubey Divyanshu , Niu Zhiyv , Spies Judith , Laughlin Ruple S. , Thakolwiboon Smathorn , Santilli Ashley R. , Rashed Hebatallah , Mirman Igal , Swart Alexander , Berini Sarah E. , Shouman Kamal , Pinto Marcus V. , Mauermann Michelle L. , Mills John R. , Dyck P. James B. , Harmsen William S. , Mandrekar Jay , Klein Christopher J. 
Published: January 2025

ABSTRACTBackground and AimsChronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is difficult to distinguish from mimicking disorders, with misdiagnosis resulting in IVIG overutilization. We evaluate a clinical‐electrophysiological model to facilitate CIDP versus mimic neuropathy prediction.MethodsUsing the European Academy of Neurology/Peripheral Nerve Society (EAN/PNS) 2021 CIDP guidelines we derived 26 clinical and 144 nerve conduction variables. The model was generated and validated utilizing total CIDP (n = 129) and mimics (n = 309); including (1) IgG4‐nodopathies; (2) POEMS (polyneuropathy–organomegaly–endocrinopathy–monoclonal protein‐skin changes); (3) anti‐myelin‐associated‐glycoprotein; (4) paraneoplastic; (5) Waldenström B‐cell lymphoma; (6) diabetic neuropathies; (7) amyloidosis; (8) Charcot–Marie–Tooth; (9) motor neuropathies/neuronopathies; and (10) idiopathic‐inflammatory‐myopathies.ResultsWe analyzed 9282 clinical and 51 408 electrophysiological data points. Univariate analysis identified 11 of 26 clinical variables with significant odds ratios. A multivariate regression model using four clinical and two electrophysiologic variables achieved 93% area‐under‐curve (95% CI 91–95): progression over 8 weeks (OR 40.66, 95% CI 5.31–311.36), absent autonomic involvement (OR 17.82, 95% CI 2.93–108.24), absent muscle atrophy (OR 16.65, 95% CI 3.27–84.73), proximal weakness (OR 3.63, 95% CI 1.58–8.33), ulnar motor conduction velocity slowing < 35.7 m/s (OR 5.21, 95% CI 2.13–12.76), and ulnar motor conduction block (OR 13.37, 95% CI 2.47–72.40). A web‐based probability calculator (https://news.mayocliniclabs.com/cidp‐calculator/) was developed, with 100% sensitivity and 68% specificity at a 92% probability threshold. Specificity improved to 93% when considering “red flags,” electrophysiologic criteria, and laboratory testing.InterpretationA probability calculator using clinical electrophysiological variables assists CIDP differentiation from mimics, with scores below 92% unlikely to have CIDP. The highest specificity is achieved by considering clinical “red flags,” electrophysiologic demyelination, and laboratory testing.
A Retrospective Cohort Study Comparing the Clinical Outcomes of the Hydrosurgery System and Traditional Single‐Incision Surgery for Axillary Osmidrosis
Authors: Xia Lufan , He Mu , Liu Xiaoqiang , Zhang Jinghong , Chen Ying , Zhang Jiaping 
Published: January 2025

ABSTRACTPurposeTo compare the postoperative scarring, complication rates, and efficacy between the hydrosurgery system and traditional single‐incision surgical techniques for treating axillary osmidrosis.MethodsA retrospective collection was conducted of all patients who underwent radical surgery for axillary osmidrosis at the Day Surgery Unit of the Department of Plastic Surgery at the First Affiliated Hospital of the Army Medical University from January 2023 to January 2024. Patients were screened based on inclusion and exclusion criteria and divided into the hydrosurgery group and the traditional surgery group. The evaluation was done through medical records, follow‐up records, electronic questionnaires, and photographs. Assessments included 6‐month postoperative scarring conditions, comprehensive effectiveness scores, postoperative odor/hair/sweating scores, postoperative dermatological life quality scores, and complication rates.ResultsA total of 73 patients completed this study: 34 in the hydrosurgery group and 39 in the traditional surgery group. 6 months postoperatively, the hydrosurgery group had significantly lower scores of the Vancouver Scar Scale (VSS), 0.5 (range 0.0–2.0) compared with 1.5 (range 0.5–3.0) in the traditional surgery group (p = 0.018). The incidence of complications such as subcutaneous hematoma, epidermal necrosis, and wound dissidence was also lower (26.5% vs. 51.3%, p = 0.031). Additionally, the surgical incision length in the hydrosurgery group was significantly smaller than in the traditional surgery group (1.200 cm (1.000, 1.275) vs. 2.500 cm (2.500, 3.000), p < 0.001). There were no significant differences between the two groups regarding comprehensive effectiveness scores, postoperative odor/hair/sweating scores, postoperative dermatological life quality index, and surgical duration.ConclusionCompared with traditional single‐incision surgical techniques, patients in the hydrosurgery group exhibited lower scar scores 6 months postsurgery, required smaller surgical incisions, and had lower rates of surgical complications. Thus, the hydrosurgery is suitable for the minimally invasive surgical treatment of axillary osmidrosis, offering a safe, effective, and aesthetically superior treatment method.
School‐Based Physical Activity Program Implementation Is Enhanced With Support From Community‐Based Partners
Authors: Packebush Thomas , Ahern Katherine , Brody Barbara , Gunter Katherine 
Published: January 2025

ABSTRACTBackgroundSchool‐based physical activity (PA) programs are an equitable, evidence‐based approach to combat health and PA disparities. This study examined factors associated with implementation of BE Physically Active 2Day (BEPA 2.0), a K‐5 school‐based PA program, and examined how support from Cooperative Extension via Supplemental Nutrition Assistance Program Education (SNAP‐Ed) partners influenced implementation.MethodsTeachers in schools receiving BEPA 2.0 trainings were surveyed about program use 3–6 months post‐training. Descriptive and chi‐square analyses were conducted to evaluate the relationships between implementation factors (e.g., training, support, barriers) and program delivery. Logistic regression was used to summarize the odds of program delivery related to implementation factors.FindingsResponses were returned by 355 teachers. The odds of program delivery among trained respondents were 2.64 (1.22, 5.47) times the odds among untrained respondents. Most implementors (72.4%) provided activities 1–2 times/week. More frequent implementation (≥ 3 times/week) [OR = 2.43 (1.27, 4.73)] and fewer barriers were reported among those who received support from SNAP‐Ed partners (p = 0.01).Implications for School Health Policy, Practice, and EquitySNAP‐Ed program partnerships should be considered when implementing school‐based strategies to increase PA opportunities for vulnerable youth.ConclusionsHigh PA program implementation among trained teachers was enhanced with partner support.
Global and Regional Trends in Paediatric Neuroblastoma Incidence and Mortality, 1990–2021: An Inequality and Projection Analysis
Authors: Fan Yue , Zhang Jian , Liang Hao , Zhang Yong 
Published: January 2025

ABSTRACTBackgroundNeuroblastoma stands as the most prevalent extracranial solid tumour in children, yet its epidemiological profile on global, regional, and national scales remains insufficiently explored.MethodsLong‐term trends in the incidence and mortality of paediatric neuroblastoma from 1990 to 2021 were analysed globally, regionally, and nationally using estimated annual percentage changes. Cross‐national disparities in the burden of paediatric neuroblastoma were quantified through standard health equity methodologies, with projections of burden shifts extending to 2035.ResultsFrom 1990 to 2021, the global age‐standardised death rate for paediatric neuroblastoma increased from 0.19 (95% UI, 0.16–0.23) to 0.21 (95% UI, 0.14–0.28) per 100 000 population, with an EAPC of 0.2. Regionally, both mortality and incidence rates were highest in low‐middle SDI regions and lowest in Oceania. Mortality rates were highest among males aged 12–23 months and females aged 6–11 months, while incidence rates were most pronounced in children aged 2–4 years. Cross‐country inequality analyses indicated a reduction in disparities between high and low SDI countries; however, the concentration index revealed an increase in the inequality of burden distribution. The global burden of paediatric neuroblastoma is projected to continue rising through 2035.ConclusionsOver the past 30 years, global incidence and mortality rates for paediatric neuroblastoma have risen, particularly in low‐SDI regions, whereas high‐SDI regions have witnessed a downward trend. Although disparities between high‐ and low‐SDI countries have diminished, inequality in burden distribution has grown more pronounced. The global burden of paediatric neuroblastoma is expected to continue its upward trajectory over the next 15 years.
Liver GPBAR1 Associates With Immune Dysfunction in Primary Sclerosing Cholangitis and Its Activation Attenuates Cholestasis in Abcb4−/− Mice
Authors: Di Giorgio Cristina , Urbani Ginevra , Marchianò Silvia , Biagioli Michele , Bordoni Martina , Bellini Rachele , Massa Carmen , Lachi Ginevra , Cari Luigi , Morretta Elva , Spinelli Lucio , Monti Maria Chiara , Sepe Valentina , Zampella Angela , Distrutti Eleonora , Banales Jesus M. , Lapitz Ainhoa , Milkiewicz Piotr , Milkiewicz Malgorzata , Fiorucci Stefano 
Published: January 2025

ABSTRACTBackground and AimsPrimary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterised by progressive biliary inflammation and fibrosis, leading to liver cirrhosis and cholangiocarcinoma. GPBAR1 (TGR5) is a G protein‐coupled receptor for secondary bile acids. In this study, we have examined the therapeutic potential of BAR501, a selective GPBAR1 agonist in a PSC model.MethodsSingle‐cell analysis of healthy human liver samples and gene expression analysis of PSC liver samples were conducted. In vitro studies on a human cholangiocyte cell line (NHC), U937 and human hepatic stellate cells (hSteCs) were performed. Additionally, Abcb4−/− mice were treated with BAR501 for 12–24 weeks.ResultsSingle‐cell analysis demonstrated that GPBAR1 is expressed by macrophages, NK cells, sinusoidal cells and to a lesser extent by cholangiocytes. Total liver expression of GPBAR1 increases in PSC patients compared to that in healthy controls and positively correlates with markers for monocytes and NK cells and cytokeratin 19. In vitro treatment of NHCs with BAR501 reversed the acquisition of a pro‐inflammatory phenotype and the downregulation of GPBAR1 expression promoted by LPS in an NF‐κB‐dependent manner. Treating Abcb4−/− mice reduced bile duct inflammation and liver fibrosis and prevented the downregulation of GPBAR1 expression. Treating mice with BAR501 also modulated the bile acid pool composition and reduced the dysbiosis‐associated gut permeability, and intestinal and systemic inflammation. Ex vivo experiments using conditioned media from BAR501‐treated cholangiocytes mitigated the activation of macrophages.ConclusionsOur study provides evidence for the therapeutic potential of selective GPBAR1 agonists in intestinal inflammation–associated cholestasis, warranting the evaluation of BAR501 in PSC patients.
Using X‐ray computed microtomography (μCT) to determine subsample‐specific cosmogenic noble gas production rates of E (enstatite) chondrites
Authors: Mijjum M. , Andrews B. J. , McCoy T. J. , Corrigan C. M. , Caffee M. W. , Tremblay M. M. 
Published: January 2025

AbstractCosmic ray exposure (CRE) ages provide information about the parent bodies and source regions of meteorite classes. Cosmogenic noble gases are often used to quantify exposure time scales ranging from tens of ka to hundreds of Ma. The production rate of cosmogenic noble gases is primarily controlled by a meteorite's chemical composition. Historically, an average chemical composition for an entire meteorite class or subgroup was used to calculate production rates. At the scale needed for noble gas measurements, however, some meteorites exhibit mineral abundance variabilities that translate into chemical heterogeneities, necessitating subsample‐specific production rates. We find that the metal and sulfide content can vary significantly between ~100 and 300 mg subsamples of the same enstatite (E) chondrite, leading to >10% differences in cosmogenic 21Ne production rates between subsamples. We demonstrate an approach to determining subsample‐specific production rates using E chondrites. We use electron microprobe analysis and X‐ray computed microtomography to quantify the chemical composition and abundances, respectively, of metal, sulfide, and silicate minerals in six E chondrites and calculate subsample‐specific production rates of 3He and 21Ne. By applying this method to more E chondrite subsamples alongside noble gas measurements, we may begin to address broader questions, such as whether peaks in the E chondrite CRE age distribution can be attributed to distinct impact events.
Spiny mice (Acomys) have evolved cellular features to support regenerative healing
Authors: Allen Robyn S. , Seifert Ashley W. 
Published: January 2025

AbstractSpiny mice (Acomys spp.) are warm‐blooded (homeothermic) vertebrates whose ability to restore missing tissue through regenerative healing has coincided with the evolution of unique cellular and physiological adaptations across different tissue types. This review seeks to explore how these bizarre rodents deploy unique or altered injury response mechanisms to either enhance tissue repair or fully regenerate excised tissue compared to closely related, scar‐forming mammals. First, we examine overall trends in healing Acomys tissues, including the cellular stress response, the ability to activate and maintain cell cycle progression, and the expression of certain features in reproductive adults that are normally associated with embryos. Second, we focus on specific cell types that exhibit precisely regulated proliferation to restore missing tissue. While Acomys utilize many of the same cell types involved in scar formation, these cells exhibit divergent activation profiles during regenerative healing. Considered together, current lines of evidence support sustained deployment of proregenerative pathways in conjunction with transient activation of fibrotic pathways to facilitate regeneration and improve tissue repair in Acomys.
Engaging with young professionals in transfusion medicine: Insights from a needs assessment survey targeting an international cohort
Authors: McGowan Eunike C. , Lewin Antoine , Ou‐Yang Jian , Mandal Saikat , Almozain Nour , Ambaye Abiy Belay , Ribeiro Jannison Karlly Cavalcante , Boateng Lilian Antwi , Dinardo Carla Luana , Langi Sasongko Praiseldy ,   the Young Professionals Council
Published: January 2025

AbstractBackground and ObjectivesProfessionals who work or study in transfusion medicine under 40 years of age are considered young professionals (YPs) by the International Society of Blood Transfusion (ISBT). While the ISBT provides opportunities for YPs, their needs have to be assessed to customize initiatives in a way that could potentially improve their engagement. This survey aimed to assess the needs of YPs in transfusion medicine and understand their perspectives on future ISBT initiatives.Materials and MethodsBetween January and February 2023, a 28‐question online survey was accessible through a generalized link across the ISBT network. Skip‐logic responses from 352 YPs, including 151 ISBT members and 201 non‐members, were analysed. Each question varied in the number of responders and, consequently, the number of responses.ResultsFirstly, the most important needs of YPs from the survey were educational opportunities and training programmes, with 70% of respondents indicating for educational content in specific fields of transfusion. Secondly, Transfusion Today published by the ISBT (46.9%) ranked the highest in engagement, while ISBT Academy and Academy funding ranked the lowest (12.8%). ISBT members reported attending ISBT activities or using ISBT resources more often than non‐members, although this was not statistically significant. The primary barrier preventing both non‐members and ISBT members from engaging in ISBT activities was a lack of awareness.ConclusionRaising awareness on a regular basis, a customized communication style (e.g., by a representative or different languages) and activities for non‐members may be key to improving YP engagement and expanding the ISBT network.