bluuebunny/crossref_metadata_2025_split huggingface dataset:
Central markers of neuroinflammation in alcohol use disorder: A meta‐analysis of neuroimaging, cerebral spinal fluid, and postmortem studies
Authors: Adams Claire , Perry Nina , Conigrave James , Hurzeler Tristan , Stevens Julia , Yacou Dunbar Kristiane P. , Sweeney Alicia , Lee Kylie , Sutherland Greg , Haber Paul , Morley Kirsten C. 
Published: March 2023

AbstractIntroduction and aimsThere is emerging evidence that heavy long‐term alcohol consumption may alter the neuroimmune profile. We conducted a meta‐analysis of the association between alcohol use disorder (AUD) and the extent of neuroinflammation using cerebrospinal (CSF), PET (Positron Emission Tomography), and postmortem studies.Design and methodsA comprehensive search of electronic databases was conducted using the Preferred Reporting Items for Systematic Review and Meta‐Analysis Protocols (PRISMA‐P) for AUD‐related terms in combination with neuroinflammatory markers and cytokine‐ and chemokine‐related terms for CSF, PET, and postmortem studies. Participants had to meet established criteria for AUD and/or heavy alcohol consumption with dependence features and be compared with healthy controls. Papers retrieved were assessed for inclusion criteria and a critical appraisal was completed using the Newcastle‐Ottawa Scale. A meta‐analysis was conducted on postmortem and PET studies.ResultsEleven papers met the inclusion criteria with CSF, PET, and postmortem studies included in the final analysis. Postmortem studies demonstrate significant heterogeneity (𝑄 (14) = 62.02, 𝑝 < 0.001), with the alcohol group showing higher levels of neuroimmune markers than controls (𝑑 = 1.50 [95% CI 0.56, 2.45]). PET studies demonstrated a lower [11C] PBR28 total volume of distribution (V
T) for translocator protein in the hippocampus (g = −1.95 [95% CI −2.72, −1.18], p < 0.001) of the alcohol group compared to controls.ConclusionThere is emerging evidence across multiple diagnostic modalities that alcohol impacts neuroimmune signaling in the human brain.
Sex differences in the relation between military sexual trauma and risk for alcohol misuse among U.S. Army Reserve and National Guard soldiers
Authors: Fillo Jennifer , Anderson Goodell Erin M. , Homish D. Lynn , Homish Gregory G. 
Published: March 2023

AbstractBackgroundMilitary sexual trauma (MST) is linked to a range of deleterious health outcomes. Extant literature has focused disproportionately on mental health sequelae of MST. Research is needed to better understand the extent to which MST contributes to alcohol misuse and related problems ‐ key issues facing service members. Additionally, sex differences in the nature and prevalence of MST may impact sequelae. The present research examined (a) the prevalence of an MST history among female and male service members, (b) relations between MST history and risk for alcohol misuse and related problems, and (c) potential sex differences in these experiences and outcomes.MethodsData from current and previous service members (334 males and 70 females) were drawn from Operation: SAFETY (Soldiers and Families Excelling Through the Years), a longitudinal study of U.S. Army Reserve and National Guard (USAR/NG) soldiers and their partners. Analyses examined the prevalence and types of MST experienced, relations between MST history and three alcohol‐related variables (total consumption, heavy drinking frequency, and alcohol‐related problems), and differences by sex.ResultsApproximately one‐third (33.7%) of service members reported an MST history. Females (61.4%) reported an MST history at more than twice the rate of males (27.8%). Interactions between MST and sex were significant for all alcohol‐related variables. Among males, an MST history was related to 70% higher alcohol consumption, 86% higher heavy drinking frequency, and 45% higher alcohol problem scores. An MST history was unrelated to alcohol‐related variables among females.ConclusionFindings suggest a higher prevalence of MST among USAR/NG service members than is often reported in the literature, particularly among males. An MST history was associated with higher and more hazardous/harmful current alcohol consumption among male service members, highlighting the need for greater screening, prevention, and intervention among a population already at high risk for problematic alcohol use. Results underscore that MST is an important and prevalent experience requiring greater study alongside more traditional notions of service‐connected trauma.
Surgical, survival and quality of life outcomes in over 1000 pelvic exenterations: lessons learned from a large Australian case series
Authors: Steffens Daniel , Solomon Michael J. , Lee Peter , Austin Kirk , Koh Cherry , Byrne Christopher , Karunaratne Sascha , Hatcher Sophie , Taylor Kiera , McBride Kate 
Published: March 2023

AbstractBackgroundTo determine surgical, survival and quality of life outcomes across different tumour streams and lessons learned over 28 years.MethodsConsecutive patients undergoing pelvic exenteration at a single, high volume, referral hospital, between 1994 and 2022 were included. Patients were grouped according to their tumour type at presentation as follows, advanced primary rectal cancer, other advanced primary malignancy, locally recurrent rectal cancer, other locally recurrent malignancy and non‐malignant indications. The main outcomes included, resection margins, postoperative morbidity, long‐term overall survival, and quality of life outcomes. Non‐parametric statistics and survival analyses were performed to compare outcomes between groups.ResultsOf the 1023 pelvic exenterations performed, 981 (95.9%) unique patients were included. Most patients underwent pelvic exenteration due to locally recurrent rectal cancer (N = 321, 32.7%) or advanced primary rectal cancer (N = 286, 29.2%). The rates of clear surgical margins (89.2%; P < 0.001) and 30‐days mortality were higher in the advanced primary rectal cancer group (3.2%; P = 0.025). The 5‐year overall survival rates were 66.3% in advanced primary rectal cancer and 44.6% in locally recurrent rectal cancer. Quality of life outcomes differed across groups at baseline, but generally had good trajectories thereafter. International benchmarking revelled excellent comparative outcomes.ConclusionsThe results of this study demonstrate excellent outcomes overall, but significant differences in surgical, survival and quality of life outcomes across patients undergoing pelvic exenteration due to different tumour streams. The data reported in this manuscript can be utilized by other centres as benchmarking as well as proving both subjective and objective outcome details to support informed decision‐making for patients.
Research on biomechanical compatibility for a novel artificial anal sphincter with constant force
Authors: Wang Minghui , Liu Yunlong , Liao Yucheng , Yu Hongliu 
Published: March 2023

AbstractBackgroundExisting artificial anal sphincter studies have shown that biomechanical compatibility problem between artificial anal sphincter and rectum caused by long‐term morphological changes of the tissue surrounding the implanted prosthesis can lead to device failure or tissue ischemic necrosis. In this article, a mechanical artificial anal sphincter with constant force clamping is designed based on the superelasticity of shape memory alloys, which improved the biomechanical compatibility of implantable artificial anal sphincter.MethodsFirstly, the anatomical structure and the biomechanical properties of the rectum are analyzed to obtain the size parameters and material parameters of the rectal model. Secondly, a novel artificial anal sphincter with constant force is designed to improve the biomechanical compatibility between the artificial sphincter and the rectum. Thirdly, the static analysis of artificial anal sphincter is carried out by finite element analysis.ResultsThe simulation results show that the artificial anal sphincter can maintain a constant clamping force of 4 N within a certain variation range of intestinal tissue thickness, which verifies the constant force characteristic of the artificial anal sphincter. The constant clamping force of the artificial anal sphincter to the rectum is 4 N that is greater than the clamping force 3.99 N required to close the rectum, which verifies the effectiveness the artificial anal sphincter. The surface contact stress and the minimum principal stress of the rectum in the clamping state are less than the pressure threshold, which verifies the safety of the artificial anal sphincter.ConclusionsThe novel artificial anal sphincter has better biomechanical compatibility and improves the mechanical match between artificial sphincter and intestinal tissue. This study may provide more reasonable and effective simulation data for in vivo experiments of artificial anal sphincter in future, which may provide theoretical and technical support for further research about clinical application of artificial anal sphincter.
Phoenician–Punic amphorae in northern coastal Etruria: New evidence from Pisa (Italy)
Authors: Taccola Emanuele , Miriello Domenico , De Luca Raffaella , Cicogna Francesca , Coiai Serena , Girimonte Genea , Raneri Simona 
Published: March 2023

AbstractThis study outlines the circulation of Phoenician–Punic amphorae in northern coastal Etruria, with a particular focus on Pisa (Italy), where their presence has been attested since the mid‐eighth century BCE. A set of specimens from Piazza del Duomo was analysed by minero‐petrographic and geochemical techniques. The results were compared with literature data from Mediterranean production areas. The research allowed a better definition of the role of Etruscan Pisa in the frame of commercial and cultural routes in the Mediterranean, specifically in the Tyrrhenian area, also providing the opportunity to review the attestations of Phoenician–Punic amphora on a regional scale.
Serum angioneurin levels following electroconvulsive therapy for mood disorders
Authors: Zhang TianHong , Tang XiaoChen , Wei YanYan , Xu LiHua , Hu YeGang , Cui HuiRu , Zeng JiaHui , Ye JiaYi , Xie YuOu , Tang YingYing , Liu HaiChun , Chen Tao , Li ChunBo , Liu XiaoHua , Wang JiJun 
Published: March 2023

AbstractObjectivesThe efficacy of electroconvulsive therapy (ECT) in treating mood disorders (MDs) is hypothesized to be mediated by the induction of neurotrophic factors (denoted “angioneurins”) that trigger neuronal plasticity. This study aimed to assess the effects of ECT on serum angioneurin levels in patients with MD.MethodsA total of 110 patients with MDs including 30 with unipolar depression, 25 with bipolar depression (BD), 55 with bipolar mania (BM), and 50 healthy controls were included in the study. Patients were subdivided into two groups: those who received ECT + medication (12 ECT sessions) and those who received only medication (no‐ECT). Depressive and manic symptom assessments and measurements of vascular endothelial growth factor (VEGF), fibroblast growth factor‐2, nerve growth factor (NGF), and insulin‐like growth factor‐1 levels in blood samples were performed at baseline and week 8.ResultsPatients in the ECT group, specifically those with BD and BM, had significantly increased levels of VEGF compared to their baseline VEGF levels (p = 0.002). No significant changes in angioneurin levels were observed in the no‐ECT group. Serum NGF levels were significantly associated with a reduction in depressive symptoms. Angioneurin levels were not associated with manic symptom reduction.ConclusionsThis study hints that ECT may increase VEGF levels with angiogenic mechanisms that amplify NGF signaling to promote neurogenesis. It may also contribute to changes in brain function and emotional regulation. However, further animal experiments and clinical validation are needed.
Inhibition of protein kinase C delta leads to cellular senescence to induce anti‐tumor effects in colorectal cancer
Authors: Shimoyama Yuya , Yamada Kohji , Yoshida Saishu , Kawamura Akira , Hannya Yoshito , Imaizumi Yuta , Kumamoto Tomotaka , Takeda Yasuhiro , Shimoda Masayuki , Eto Ken , Yoshida Kiyotsugu 
Published: March 2023

AbstractProtein kinase C delta (PKCδ) is a multifunctional serine–threonine kinase implicated in cell proliferation, differentiation, tumorigenesis, and therapeutic resistance. However, the molecular mechanism of PKCδ in colorectal cancer (CRC) remains unclear. In this study, we showed that PKCδ acts as a negative regulator of cellular senescence in p53 wild‐type (wt‐p53) CRC. Immunohistochemical analysis revealed that PKCδ levels in human CRC tissues were higher than those in the surrounding normal tissues. Deletion studies have shown that cell proliferation and tumorigenesis in wt‐p53 CRC is sensitive to PKCδ expression. We found that PKCδ activates p21 via a p53‐independent pathway and that PKCδ‐kinase activity is essential for p21 activity. In addition, both repression of PKCδ expression and inhibition of PKCδ activity induced cellular senescence‐like phenotypes, including increased senescence‐associated β‐galactosidase (SA‐β‐gal) staining, low LaminB1 expression, large nucleus size, and senescence‐associated secretory phenotype (SASP) detection. Finally, a kinase inhibitor of PKCδ suppressed senescence‐dependent tumorigenicity in a dose‐dependent manner. These results offer a mechanistic insight into CRC survival and tumorigenesis. In addition, a novel therapeutic strategy for wt‐p53 CRC is proposed.
Recent advances in extracellular vesicles in gastrointestinal cancer and lymphoma
Authors: Otsuka Motoyuki , Kotani Ai 
Published: March 2023

AbstractExtracellular vesicles (EVs) are intercellular communication agents that transfer microRNAs (miRNAs), other non‐coding RNAs (ncRNAs), messenger RNAs (mRNAs), proteins, lipids, metabolites, and other molecules from donor cells (e.g., cancer cells) to recipient cells (e.g., stromal cells). In 2007, miRNAs were reported to be abundant among the ncRNAs present in EVs. Since then, many studies have investigated the functions of miRNAs and have attempted to apply these molecules to aid in the diagnosis and treatment of cancer. Research on EVs has expanded, particularly in the field of cancer, because cancer cells heavily secrete EVs. The cargo of these EVs, especially those in small EVs, such as exosomes, is assumed to work cooperatively and significantly in the tumor microenvironment and to promote metastasis. In this review, we first summarize recent studies on EVs in gastrointestinal cancer and highlight studies on human satellite II RNAs, which are a type of ncRNA found in EVs that possess repetitive sequences. Second, since several recent studies have revealed that phospholipids, which are components of EV membranes, play important roles in intercellular communication and the generation of lipid mediators in the tumor microenvironment, we review the reported roles of these molecules and discuss their potential use in the design of new cancer treatments.
ALKBH5‐induced circular RNA NRIP1 promotes glycolysis in thyroid cancer cells by targeting PKM2
Authors: Ji Xiaoyu , Lv Chengzhou , Huang Jiapeng , Dong Wenwu , Sun Wei , Zhang Hao 
Published: March 2023

AbstractAlthough circular RNAs (circRNAs) are involved in cell proliferation, differentiation, apoptosis, and invasion, the underlying regulatory mechanisms of circRNAs in thyroid cancer have not been fully elucidated. This article aimed to study the role of circRNA regulated by N6‐methyladenosine modification in papillary thyroid cancer (PTC). Quantitative real‐time PCR, western blotting, and immunohistochemistry were used to investigate the expressions of circRNA nuclear receptor‐interacting protein 1 (circNRIP1) in PTC tissues and adjacent noncancerous thyroid tissues. In vitro and in vivo assays were carried out to assess the effects of circNRIP1 on PTC glycolysis and growth. The N6‐methyladenosine mechanisms of circNRIP1 were evaluated by methylated RNA immunoprecipitation sequencing, luciferase reporter gene, and RNA stability assays. Results showed that circNRIP1 levels were significantly upregulated in PTC tissues. Furthermore, elevated circNRIP1 levels in PTC patients were correlated with high tumor lymph node metastasis stage and larger tumor sizes. Functionally, circNRIP1 significantly promoted glycolysis, PTC cell proliferation in vitro, and tumorigenesis in vivo. Mechanistically, circNRIP1 acted as a sponge for microRNA (miR)‐541‐5p and miR‐3064‐5p and jointly upregulated pyruvate kinase M2 (PKM2) expression. Knockdown of m6A demethylase α‐ketoglutarate‐dependent dioxygenase alkB homolog 5 (ALKBH5) significantly enhanced circNRIP1 m6A modification and upregulated its expression. These results show that ALKBH5 knockdown upregulates circNRIP1, thus promoting glycolysis in PTC cells. Therefore, circNRIP1 can be a prognostic biomarker and therapeutic target for PTC by acting as a sponge for oncogenic miR‐541‐5p and miR‐3064‐5p to upregulate PKM2 expression.
A survey study on early detection of young children with disabilities in urban and rural areas of China
Authors: Su Xueyun , Peng Xiaomei , Liang Lelin , Li Yinhua , Xie Huichao 
Published: March 2023

AbstractAimThis study aimed to understand how children and families access early intervention in China.BackgroundTimely identification and high‐quality intervention is expected to prevent and reduce the occurrence and severity of chronic functional impairment for children with disability and is of great significance to individuals and the society. The current study recruited 1129 caregivers of children with disabilities from rural and urban areas of China were recruited to participate in a survey.Results(a) The first concern about development was raised, usually by the parents, when a child with disabilities was 26 months of age, (b) developmental screening took place 4 months after the first concern and diagnostic evaluation happened 7 months after, (c) the types of early intervention programme varied across urban and rural areas and (d) child and family factors were found associating with age of detection.ConclusionsFindings highlight the concerningly late age of children being identified for early intervention and disparities in services between urban and rural areas in China. Implications are provided for practitioners, policy makers and future research.
Srs11‐92, a ferrostatin‐1 analog, improves oxidative stress and neuroinflammation via Nrf2 signal following cerebral ischemia/reperfusion injury
Authors: Chen Yuhua , He Wei , Wei Hong , Chang Cuicui , Yang Lingjian , Meng Jiao , Long Tianlin , Xu Quanhua , Zhang Chi 
Published: March 2023

AbstractAimFerroptosis is increasingly becoming to be considered as an important mechanism of pathological cell death during stroke, and specific exogenous ferroptosis inhibitors have the ability to reverse cerebral ischemia/reperfusion injury. However, research on Srs11‐92 (AA9), a ferrostatin‐1 (Fer‐1) analog, in preclinical studies is limited.MethodsIn the middle cerebral artery occlusion‐reperfusion (MCAO/R) mice model or oxygen–glucose deprivation/reperfusion (OGD/R) cell model, Fer‐1, AA9, and/or ML385 were administered, and brain infarct size, neurological deficits, neuronal damage, oxidative stress, and neuroinflammation were determined after the damage, in vitro and in vivo.ResultsFer‐1 and AA9 improved brain infarct size, neuronal damage, and neurological deficits in mice model of MCAO/R, and inhibited the overloaded iron deposition, ROS accumulation, and neuroinflammation response: it also increased the expression of GPx4, Nrf2, and HO‐1 and suppressed the expression of HMGB1 and NF‐κB p65 in the epicenter of injured hippocampal formation. However, Nrf2 inhibitor ML385 reversed the neuroprotective effect of AA9, including the oxidative stress and neuroinflammation. In vitro studies showed that AA9 relieved OGD/R‐induced neuronal oxidative stress and neuroinflammation via the Nrf2 pathway, which was impaired by ML385 in primary neurons.ConclusionThe findings imply that Fer‐1 analog AA9 may be suitable for further translational studies for the protection of neuronal damage via Nrf2 signal pathway‐mediated oxidative stress and neuroinflammation in stroke and others neurological diseases.
Genetic impacts on nigral iron deposition in Parkinson's disease: A preliminary quantitative susceptibility mapping study
Authors: Wu Jingjing , Guo Tao , Zhou Cheng , Bai Xueqin , Liu Xiaocao , Gu Luyan , Xuan Min , Gu Quanquan , Huang Peiyu , Song Zhe , Zhang Baorong , Xu Xiaojun , Zhang Minming , Guan Xiaojun 
Published: March 2023

AbstractBackgroundDysfunction of iron metabolism, especially in substantia nigra (SN), is widely acknowledged in Parkinson's disease (PD), but the genetic influence on iron deposition remains largely unknown. Thus, in this study, we aimed to investigate potential genetic impacts on iron deposition in PD.MethodsSeventy‐four subjects, including 38 patients with PD and 36 age‐matched normal controls, participated in this study. Imaging genetic association analysis was used to identify the specific influence of single nucleotide polymorphism (SNP) on iron‐related quantitative traits (QT). Genetic effects on iron deposition at the disease level, SNP level, and their interactive effect were highlighted.ResultsFour strong SNP–QT associations were detected: rs602201‐susceptibility of bilateral SN, rs198440‐susceptibility of left SN, and rs7895403‐susceptibility of left caudate head. Detailed analyses showed that: (1) significant iron deposition was exclusively found in bilateral SN in PD; (2) altered polymorphisms of the A allele/A− genotype of rs602201 and G allele/G− genotype of rs198440 and rs7895403 were more frequently observed in PD; (3) for rs602201, among all subjects, A− genotype carriers showed significantly increased iron content than TT genotype in bilateral SN; for rs198440 and rs7895403, G− carriers showed increased iron content than AA genotype in left SN and left caudate head, respectively; and (4) rs602201 exhibited significant SNP‐by‐disease interaction in bilateral SN.ConclusionsThis study shows that rs602201 and rs198440 have a stimulative impact on nigral iron deposition in PD, which provides improved understanding of iron‐related pathogenesis in PD, and specifically, that vulnerability to iron deposition in SN is genetic‐based.
Upregulation of Wnt2b exerts neuroprotective effect by alleviating mitochondrial dysfunction in Alzheimer's disease
Authors: Xu Ling‐Zhi , Li Bing‐Qiu , Li Fang‐Yu , Quan Mei‐Na , Qin Wei , Li Ying , Li Wen‐Wen , Zhao Yu , Wei Yi‐Ping , Jia Jian‐Ping 
Published: March 2023

AbstractAimsThis study investigated the relationship between plasma Wnt2b levels and Alzheimer's disease (AD), and explored the effect of Wnt2b on mitochondrial dysfunction in AD.MethodsHealthy and AD subjects, AD transgenic mice, and in vitro models were used to investigate the roles of Wnt2b in abnormalities in canonical Wnt signaling and mitochondria in AD. RT‐qPCR, immunoblotting, and immunofluorescence analysis were performed to assay canonical Wnt signaling. Mitochondrial structure was analyzed by electron microscopy. Flow cytometry was used to examine the intracellular calcium and neuronal apoptosis.ResultsPlasma Wnt2b levels were lower in AD patients and positively correlated with cognitive performance. Similarly, Wnt2b was reduced in the hippocampus of AD mice and in vitro models. Next, Wnt2b overexpression and recombinant Wnt2b were used to endogenously and exogenously upregulate Wnt2b levels. Upregulation of Wnt2b could effectively prevent downregulation of canonical Wnt signaling, mitochondrial dysfunction in in vitro AD models. Subsequently, intracellular calcium overload and neuronal damage were ameliorated.ConclusionsOur study highlights that Wnt2b decline is associated with cognitive impairment in AD, and upregulation of Wnt2b can exert neuroprotective effects in AD, particularly in ameliorating mitochondrial dysfunction.