bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sodium regulates PLC and IP3R‐mediated calcium signaling in invasive breast cancer cells
Authors: James Andrew D. , Unthank Katherine P. , Jones Isobel , Sajjaboontawee Nattanan , Sizer Rebecca E. , Chawla Sangeeta , Evans Gareth J. O. , Brackenbury William J. 
Published: April 2023

AbstractIntracellular Ca2+ signaling and Na+ homeostasis are inextricably linked via ion channels and co‐transporters, with alterations in the concentration of one ion having profound effects on the other. Evidence indicates that intracellular Na+ concentration ([Na+]i) is elevated in breast tumors, and that aberrant Ca2+ signaling regulates numerous key cancer hallmark processes. The present study therefore aimed to determine the effects of Na+ depletion on intracellular Ca2+ handling in metastatic breast cancer cell lines. The relationship between Na+ and Ca2+ was probed using fura‐2 and SBFI fluorescence imaging and replacement of extracellular Na+ with equimolar N‐methyl‐D‐glucamine (0Na+/NMDG) or choline chloride (0Na+/ChoCl). In triple‐negative MDA‐MB‐231 and MDA‐MB‐468 cells and Her2+ SKBR3 cells, but not ER+ MCF‐7 cells, 0Na+/NMDG and 0Na+/ChoCl resulted in a slow, sustained depletion in [Na+]i that was accompanied by a rapid and sustained increase in intracellular Ca2+ concentration ([Ca2+]i). Application of La3+ in nominal Ca2+‐free conditions had no effect on this response, ruling out reverse‐mode NCX activity and Ca2+ entry channels. Moreover, the Na+‐linked [Ca2+]i increase was independent of membrane potential hyperpolarization (NS‐1619), but was inhibited by pharmacological blockade of IP3 receptors (2‐APB), phospholipase C (PLC, U73122) or following depletion of endoplasmic reticulum Ca2+ stores (cyclopiazonic acid). Thus, Na+ is linked to PLC/IP3‐mediated activation of endoplasmic reticulum Ca2+ release in metastatic breast cancer cells and this may have an important role in breast tumors where [Na+]i is perturbed.
A randomized controlled trial to evaluate the efficacy of electrical vestibular nerve stimulation (VeNS), compared to a sham control for the management of sleep in young adults
Authors: Goothy Sai Sailesh Kumar , Vijayaraghavan Rajagopalan , Chakraborty Hirok 
Published: April 2023

Abstract

Objectives
Preliminary research suggests that electrical vestibular nerve stimulation (VeNS) may improve sleep outcomes by influencing the hypothalamus and brainstem nuclei involved in regulating the circadian rhythm and wakefulness. This randomised, sham-controlled trial aimed to assess the effectiveness of VeNS on insomnia in young adults.

Methods
Eighty adults aged 18–24 years were randomly allocated to the intervention (n=40) and control groups (n=40). The intervention group was provided with 30 min per day of VeNS with five sessions weekly for four weeks, while the control group received sham stimulation for the same period. Baseline Insomnia Sleep Index (ISI) scores were recorded weekly. At baseline and at day 28, questionnaires to evaluate emotional states of depression, anxiety and stress, and quality of life (QoL) were completed. The primary outcome was change in ISI with comparison between baseline and day 28.

Results
The VeNS group significantly reduced their mean ISI score after 7 days usage (p<0.001). At day 28 it was found that mean ISI scores had reduced from 19 to 11 in the VeNS group, and from 19 to 18 in the sham group, and the difference between the groups was significant (p<0.001). Moreover, application of VeNS appeared to significantly improve emotional state and QoL outcomes.

Conclusions
This trial demonstrates that regular VeNS usage over four weeks leads to a clinically meaningful decrease in ISI scores in young adults with insomnia. VeNS may have potential as a drug-free and non-invasive therapy to improve sleep outcomes by positively influencing the hypothalamic and brainstem nuclei.
A Quality Improvement Project to Reduce Antibiotic Exposure in Premature Neonates
Authors: Fischer Ashley Michelle , Mitchell Jennifer L. , Stanley Kaylee C. , Javed M. Jawad 
Published: April 2023

BACKGROUND AND OBJECTIVES
Overuse of antibiotics in NICUs is a problem worldwide. Unnecessary antibiotic exposure leads to resistance, changes in the microbiome, and increases the risk of bronchopulmonary dysplasia, retinopathy of prematurity, periventricular leukomalacia, necrotizing enterocolitis, late-onset sepsis (LOS), and mortality in neonates. We aimed to safely reduce the antibiotic usage rate (AUR) in our level IV unit by 10% by December 2018.

METHODS
A multidisciplinary quality improvement project took place as part of a Vermont Oxford Network initiative in 2018. Multiple interventions took place, including identification of variations in practices and subsequent standardization through the creation of early onset and LOS guidelines, mass education, improved visibility of the guidelines, and standardized documentation. The main outcome measure for this project was the AUR for infants born &lt;35 weeks’ gestation expressed as antibiotic doses per 1000 patient days.

RESULTS
The AUR decreased from a mean of 524 to 394, for a decrease of 24.8%. Results have been sustained for 3 years. Main contributors that led to the sustained success include decreasing the overall use of antibiotics for early onset sepsis, as well as the duration when cultures are negative. The number of LOS courses also decreased slightly. We noted no cases of inadequately treated sepsis resulting in subsequent positive cultures.

CONCLUSIONS
Creation of guidelines with mass education and ongoing feedback/monitoring can result in a safe reduction of AUR in the NICU.
COVID–19 és onkohematológia
Authors: Udvardy Miklós , Illés Árpád 
Published: April 2023

Összefoglaló. Az áttekintő közlemény foglalkozik a legfontosabb
olyan helyzetekkel, amely a koronavírus-betegség (COVID–19) pandémia idején a
kemo- és biológiai terápiás irányelvek változását érinti leukémiában,
lymphomákban, csontvelő-transzplantációs helyzetekben. Külön figyelmet kell
fordítani és eltérő teendőket kell megfogalmazni a kezelési fázisok, például
indukció, konszolidáció, fenntartó és egyéb kezelésmódok tekintetében. Nagy
figyelmet érdemelnek a nemzetközi ajánlások, de egyben finom értékelést is
kívánnak bizonyos gyógyszerek esetében, például monoklonális antitestek,
szteroidok, innovatívabb akut leukémiás szerek, kolóniastimuláló faktorok,
JAK2-gátlók, interferonok, tirozin-kináz-gátlók stb. esetében. Foglalkozunk a
nemzetközi csontvelő-transzplantációval kapcsolatos ajánlásokkal. Mindent
nagyban befolyásol az aktuális COVID-pandémiás helyzet. Természetesen
foglalkozunk a vakcináció kérdéseivel is onkohematológiai kórképekben. A COVID
és az onkohematológia viszonyrendszere a gyakorlati cselekvések terén még mindig
tanuló fázisban van, meg kell találni az összhangot a pandémia és a klinikai
gyakorlat összehangolása tekintetében. A gyorsan változó COVID-helyzet
megkívánja, hogy ismereteinket és cselekvéseinket napi szinten frissen tartsuk,
a szokásosnál többet támaszkodjunk a tapasztalatokra, s figyeljünk oda a
standard publikációk mellett a hitelesnek vélt, s a helyzet miatt
elengedhetetlenül szükséges, gyorsan kommunikált állásfoglalásokra is.
Summary. A brief analysis of international recommendations and
applicable clinical decisions are summarized regarding the clinical care of
oncohaematological patients in the era of COVID-19 pandemic. General
precautionary measures are extremely important. Special attention is paid to
different groups of patients (i.e. leukaemias, lymphomas, transplant
situations), along with analysing different treatment phases, e.g. watch and
wait, induction, consolidation, maintenance, etc. Some recommendations are
really remarkable with some drugs and protocols, however they do need fine
tuning and careful individual decisions, e.g. monoclonal antibodies, steroids,
some innovative agents in acute myeloid leukaemia, colony stimulation, ATRA
based therapies, some 2nd line tyrosine kinase inhibitors in chronic myeloid
leukaemia, etc. Some aspects of international COVID-19 specific
transplantational guidelines are also covered. Vaccination recommendations and
experience are also in the focus. It may not be stressed enough, that we are
still in the learning phase of harmonization of COVID-19 pandemic and
oncohaematology care, this is still a moving target, depending a lot on actual
pandemic situation and periods. Considering ever changing COVID situation one
should follow peer reviewed publications as before, but of course frequently
review new COVID statements, in an effort to preserve, maintain efficiency of
endangered oncohaematological care.
Durabilité naturelle et composition en extractibles du bois de Juniperus oxycedrus subsp. oxycedrus du Nord-Ouest de l’Algérie
Authors: OUAAR Djilali , MEGHERBI BENALI Aicha , TOUMI BENALI Fawzia , THÉVENON Marie-France , CANDELIER Kévin , PIGNOLET Luc , GÉRARD Jean 
Published: April 2023

Les bois de Juniperus oxycedrus subsp. oxycedrus étudiés sont issus de deux provenances du Nord-Ouest algérien aux conditions climatiques et édaphiques différentes (régions de Sidi Bel Abbès et de Tiaret). Les extractibles et les polyphénols totaux du duramen ont été quantifiés et caractérisés. La durabilité naturelle du duramen a été évaluée vis-à-vis de deux champignons de pourriture brune (Rhodonia placenta et Coniophora puteana) et de termites (Reticulitermes flavipes). Les taux d’extractibles les plus élevés sont ceux obtenus pour les bois de Sidi Bel Abbès. L’extraction au mélange toluène-éthanol a permis d’obtenir des taux plus élevés que l’extraction à l’eau, respectivement 14,17 % et 1,35 % pour les bois de Sidi Bel Abbès contre 9,84 % et 1,19 % pour ceux de Tiaret. Les analyses par chromatographie en phase gazeuse couplée à la spectrométrie de masse (GC/MS) montrent que les familles chimiques majoritairement identifiées dans les extraits aqueux sont les sesquiterpénoïdes, les acides gras et les stérols. Dans les extraits au toluène-éthanol, les sesquiterpènes et les sesquiterpénoïdes sont les composés majeurs. La composition en sesquiterpènes et sesquiterpénoïdes est différente en fonction de la provenance des bois, mais reste très similaire quantitativement. La teneur en polyphénols totaux des extraits au méthanol de la provenance Sidi Bel Abbès est supérieure à celle de Tiaret. Aucune différence significative n’est observée entre les deux provenances pour la durabilité naturelle vis-à-vis de C. puteana. En revanche, la résistance à R. placenta est significativement plus élevée pour la provenance Sidi Bel Abbès. Pour les deux provenances, le bois de J. oxycedrus subsp. oxycedrus est très durable vis-à-vis des champignons de pourriture. Il est aussi répulsif et résistant aux attaques de termites. Une analyse ciblée des relations entre la composition chimique et la durabilité naturelle permettra de mettre en évidence la nature du ou des constituants responsables de la durabilité́ naturelle du bois de J. oxycedrus subsp. oxycedrus, tant vis-à-vis des champignons lignivores que des termites.
Significant serum proteinic and metabolic alterations in hepatitis B cirrhosis patients treated with umbilical cord mesenchymal stem cells
Authors: Feng Tianhang , Shang Jin , Lai Chunyou , Yao Yutong , Zhong Deyuan , Yang Qinyan , Wang Sijia , Shi Ying , Huang Xiaolun 
Published: April 2023

AbstractStem cells based-treatment is considered as an effective regenerative therapy for liver cirrhosis patients. Data about impact of stem cell transplantation on circulating metabolites in liver cirrhosis patients remains limited. In this study, eligible participants with hepatitis B cirrhosis were injected with 10E7 umbilical cord mesenchymal stem cells (uc-MSCs) through ultrasound-guided percutaneous liver puncture and portal vein catheterization. Changes of serum samples after treatment were measured by performing proteomics and metabolomics. As illustrated, proteins enriched in ECM remodeling were significantly altered, such as 20S proteasome subunits and laminin. Types of circulating phosphatidylcholine were augmented in patients after uc-MSCs treatment compared to original levels (14:0, 16:1, 18:1, 18:2, 20:1, 20:4), whereas phosphatidylethanolamine was diminished (20:4). Changes of serum profiles demonstrated that uc-MSC treatment is efficient in repairing tissue damage and metabolic disorders, revealing early biomarkers in clinical evaluation of uc-MSC treatment in liver cirrhosis patients.
Spatial spillover effect of Green Finance and Clean Energy on China's Provincial Green Economic Development
Authors: Zhang Minglong , Zheng Li , Xie Ying 
Published: April 2023

Abstract

Green finance is a new financing mode that pays attention to environmental benefits. Achieving a balance between the economy and the environment requires clean energy. For formulating policies to achieve sustainable development goals, it is very important to explore whether integrating green finance and clean energy is conducive to promoting green economic development (GED). This study applies a non-radial directional distance function (NDDF) to assess China's provincial GED using panel data from 2007 to 2020. It adopts the spatial Durbin model to empirically analyze the spatial spillover effects of green finance and clean energy on GED. The results indicate that: 1) The influence of green finance on GED shows a "U" curve relationship of first suppression and then rise. 2) A 1% increase in the synergy between green finance and clean energy yields a 0.1712% raise in the local GED and a 0.3482% boost to the surrounding area's GED through spatial spillovers. 3) The integration of green credit and clean energy has an apparent spatial spillover effect, and the interaction between green securities and clean energy can promote local GED. This study suggests that the government should accelerate and improve the development of a green financial market and establish a long-term linkage and co-ordination mechanism for the promotion of GED. It is crucial that financial institutions allocate more resources to clean energy development, and all regions will have to utilize the spatial spillover effect of clean energy to facilitate China's economic transformation and development in theory and practice.
Possible role of apoptosis and autophagy on mediation of tramadol induced neurodegeneration in rat hippocampus
Authors: Gholami Mina , Jamaati Hamidreza , Motaghinejad Majid , Ulloa Luis 
Published: April 2023

Abstract
Background
Tramadol (TRA) is a pain killer, which its abuse is widely increased during recent years, but clear mechanism for induction of neurotoxicity remains unclear. The present study aims to investigate involvement of apoptosis and autophagy signaling pathways and also mitochondrial system on TRA induced neurotoxicity.
Materials and Methods
Sixthy adult male rats were randomly divided into five groups that received standard saline and TRA in doses of 25, 50, 75, 100 and 150 mg/kg as intraperitoneal administration for 21 days, respectively. In 22th day, Open Field Test (OFT), as standard test for hippocampal cell damages was used. Also hippocampal level of JNK, Bcl-2, Beclin1 and Bax proteins as well as mitochondrial quadruple complex enzymes was measured
Results
TRA at doses 75,100 and 150 mg/kg causes dysfunction in OFT behavioral and also in mentioned high doses could increases level of both activated (total) and non-activated from of JNK and also increased Beclin-1 and Bax. TRA at doses of 75,100 and 150 mg/kg increased phosphorylated form of Bcl-2 level while decreased un-phosphorylated (total form) form of Bcl-2.
Conclusion
According to obtained data, TRA causes activation of apoptosis and or autophagy processes via modulation of TNF-α or IL-1β/JNK/Bcl-2/Beclin1 and Bcl-2/Bax signaling pathway and causes dysfunction of mitochondrial respiratory chain enzymes.
The weakening AMOC under extreme climate change
Authors: Madan Gaurav , Gjermundsen Ada , Iversen Silje Christine , LaCasce Joseph Henry 
Published: April 2023

Abstract
We examine changes in the Atlantic Meridional Overturning Circulation (AMOC) in the quadrupled CO2 experiments conducted under the sixth Coupled Model Intercomparison Project (CMIP6). The increase in CO2 triggers extensive Arctic warming, causing widespread melting of sea ice. The resulting freshwater spreads southward, first from the Labrador Sea and then the Nordic Seas, and proceeds southward along the eastern coast of North America. The freshwater enters the subpolar gyre north of the separated Gulf Stream, decreasing the density contrast across the current. The current weakens in response, reducing the inflow to the deepwater production regions. The AMOC cell weakens in tandem, first near the North Atlantic Current and then spreading to higher and lower latitudes. This contrasts with the common perception that freshwater caps the convection regions stifling deep water production; rather, it is the inflow to the subpolar gyre which is suppressed. Changes in surface temperature have a much weaker effect, and there are no consistent changes in local or remote wind forcing among the models. Thus an increase in freshwater discharge, primarily from the Labrador Sea, is the precursor to AMOC weakening in these simulations.
A field-enhancement optical fiber SPR sensor using graphene, molybdenum disulfide, and zinc oxide
Authors: Carvalho Igor , Xavier Renata , Fim Fabiana , Moreira Cleumar , Cruz Rossana 
Published: April 2023

Abstract
Graphene, molybdenum disulfide (MoS2), and zinc oxide (ZnO) are proposed here to enhance the evanescent field of an optical fiber Surface Plasmon Resonance (SPR) sensor. Gold and silver are the plasmonic materials and the fiber core material is polymethylmethacrylate (PMMA). A Fresnel equations-based analysis is used, and the obtained results pointed out higher values of sensitivity, figure of merit, and FWHM (Full-Width at Half Maximum) when compared to conventional SPR sensors. In despite of silver-only based SPR sensor has a better performance, oxidation occurs, and the sensor's lifetime is reduced. The addition of graphene layers leads to sensitivity values 50% higher than the conventional sensor. On the other hand, the MoS2-based sensor improves the interaction of the sensor with the bio-recognition molecules, which is attractive for biomedical applications. When ZnO was added to the silver-based sensor a highest sensitivity, 4,740.9 nm/RIU, was obtained. Graphene-based silver SPR exhibit the highest FOM values.
The value of serum D-dimer and tumor markers in predicting venous thromboembolism in elderly patients with stage III-IV lung adenocarcinoma
Authors: Wang Yunfei , Wu Jizhen , Huang Gairong , Wang Fei 
Published: April 2023

AbstractObjective To investigate the predictive value of serum D-dimer levels combined with tumor markers for venous thromboembolism (VTE) in elderly patients with stage III-IV lung adenocarcinomas. Methods A retrospective analysis was conducted involving elderly patients with inoperable stage III-IV lung adenocarcinoma admitted to the Department of Geriatrics and Department of Respiratory and Critical Care Medicine at Henan Provincial People's Hospital between January 2019 and January 2021. Patients were divided into VTE (n = 129) and non-VTE groups (n = 192). Serum D-dimer, carcinoembryonic antigen (CEA), lung cancer antigen (CYFRA21-1), carbohydrate antigen 125 (CA-125), carbohydrate antigen 199 (CA19-9), and neuron-specific enolase (NSE) levels were compared between the two groups. The risk factors for serum D-dimer and tumor markers in elderly patients with stage III-IV lung adenocarcinoma complicated by VTE were analyzed using binary logistic regression. The ROC curve was used to evaluate the predictive value of serum D-dimer and tumor markers in elderly patients with stage III-IV lung adenocarcinoma complicated by VTE. Results Compared to the non-VTE group, the serum D-dimer, CEA, CYFRA21-1, CA-125, and CA19-9 levels in elderly patients with stage III-IV lung adenocarcinoma and VTE were significantly different (P < 0.05). Further logistic regression analysis showed that D-dimer, CEA, and CYFRA21-1 levels were risk factors for VTE in elderly patients with stage III-IV lung adenocarcinoma. The area under the curve (AUC) of D-dimer, CEA, and CYFRA21-1 levels for the prediction of stage III-IV lung adenocarcinoma combined with VTE in the elderly were 0.839, 0.720, and 0.676, respectively (P < 0.05).The predicted AUC of D-dimer combined with CEA for stage III-IV lung adenocarcinoma complicated with VTE in elderly patients was 0.861, sensitivity was 0.868, specificity was 0.671, and cut-off value was 0.771. Conclusion: Serum D-dimer levels combined with the tumor marker CEA can improve the clinical diagnosis of VTE in elderly patients with stage III-IV lung adenocarcinoma.
Thermal loads in two different urban quarters – perspectives from mobile measurements and mental maps
Authors: Moderow Uta , Ziemann Astrid , Goldberg Valeri , Sinning Heidi 
Published: April 2023

Abstract

The fact that different urban structures have different climatic effects and therefore differ in their thermal loads for people is well known. However, there is a lack of quantitative and qualitative surveys in specific districts that are suitable to derive accepted adaptation measures. This paper addresses the research questions where thermally stressed areas in public space are identified by mobile measurements and by mental maps and what are the causes of each, where both methods agree or disagree, and what are the benefits and the limitations of using both methods for prioritizing adaptation measures.
Mobile measurements in an urban quarter over a whole day can supply needed data for determining thermal loads of urban structures and their temporal development. Mental maps give information about the perception of urban dwellers and - based on the spatial distribution of obtained data - the user frequency. Both methods provide information concerning where and when measures should be taken in order to facilitate adaptation to heat.
The paper presents the results of mobile measurements and mental maps of two different urban quarters in Germany. Thermal loads were assessed by using the Universal Thermal Climate Index (UTCI) for three selected summer days. Results indicated that the different urban structures can differ by up to 7 K or by two stages of thermal stress during a hot summer day. Surface material with high albedo can overcompensate smaller sky view factors resulting in high thermal loads. Street trees caused changing thermal loads but reduced them on average.
Identified hot spots based on mobile measurements mostly correlated with hot spots identified by mental maps, if they were frequently used. However, hottest spots identified by measurements were not necessarily most frequently named as hot spots in the mental maps. Most often named hot spots of mental maps coincided with major traffic routes suggesting that user frequency is important. We conclude that the combination of both methods can be valuable for identifying locations with high priority for climate adaptation in cities.
Do income levels and institutional quality mediate the impact of foreign direct investment on growth?
Authors: Gattini Luca , Baiashvili Tamar 
Published: April 2023

Abstract
We investigate the role played by country income levels and the quality of the institutional environment in mediating the impact of FDI on growth in 111 countries, stretching from developing and emerging market to advanced economies. We find that FDI benefits do not accrue evenly across country income groups. The effect is larger for middle-income countries while we detect mixed evidence for high- and low- income countries. We make use of this first association to characterise the impact of FDI mediated by the institutional quality of countries. We uncover that institutional factors have a mediating positive effect on FDI within country income groups, whereby countries with better-developed institutions relative to their income group peers benefit more from a positive impact of FDI on growth. From a policy perspective, FDI appears a useful tool to support growth even more so when combined with a genuine reform agenda.
JEL classification: C33, F21, E02, O43, O47