bluuebunny/crossref_metadata_2025_split huggingface dataset:
Botulinum toxin type A alleviates androgenetic alopecia by inhibiting apoptosis of dermal papilla cells via targeting circ_0135062/miR-506-3p/Bax axis
Authors: Wei Wuhan , Zhang Jingyu , Guo Yanping , Wei Hanxiao , Shen Caiqi , Zhao Bingkun , Ji Zhe , Li Qiang , Jin Peisheng 
Published: May 2023

Abstract
Botulinum toxin type A (BTXA) has been shown to be effective in androgenetic alopecia (AGA), but its effect on dermal papillary cell (DPC) apoptosis is not yet clear. Non-coding RNA plays pivotal roles in the development of AGA. In this study, we investigated the molecular mechanism of BTXA in dihydrotestosterone(DHT)-induced apoptosis of DPCs. The expressions of circ_0135062, miR-506-3p and Bax were detected by qRT-PCR. RNA pulldown and dual luciferase assays were used to analyze binding evidence. Cell viability was validated using the cell counting kit-8 assay, and cell apoptosis was determined by flow cytometry, TUNEL assay, and Western blot. Our results revealed that BTXA inhibited apoptosis of DPCs treated with DHT and that overexpression of circ_0135062 antagonized the protection of BTXA in DHT-treated DPCs. The miR-506-3p interacted with Bax, and miR-506-3p inhibited apoptosis of DPCs against DHT-mediated damage by silencing Bax. Additionally, circ_0135062 served as a miR-506-3p sponge and inhibited miR-506-3p targeting Bax expression. In conclusion, BTXA exerted an antiapoptotic effect on DHT-induced DPC injury via the circ_0135062/miR-506-3p/Bax axis.
Precision Prognostics for Cardiovascular Disease in Type 2 Diabetes: A Systematic Review and Meta-analysis
Authors: Ahmad Abrar , Lim Lee-Ling , Morieri Mario , Tam Claudia , Cheng Feifei , Chikowore Tinashe , Dudenhöffer-Pfeifer Monika , Fitipaldi Hugo , Huang Chuiguo , Kanbour Sarah , Sarkar Sudipa , Koivula Robert , Motala Ayesha , Tye Sok , Yu Gechang , Zhang Yingchai , Provenzano Michele , Sherifali Diana , de Souza Russell , Tobias Deirdre , Gomez Maria , Ma Ronald , PMDI ADA/EASD , Mathioudakis Nestoras 
Published: May 2023

Abstract
Background:
Precision medicine has the potential to improve cardiovascular disease (CVD) risk prediction in individuals with type 2 diabetes (T2D).
Methods:
We conducted a systematic review and meta-analysis of longitudinal studies to identify potentially novel prognostic factors that may improve CVD risk prediction in T2D. Out of 9380 studies identified, 416 studies met inclusion criteria. Outcomes were reported for 321 biomarker studies, 48 genetic marker studies, and 47 risk score/model studies.
Results:
Out of all evaluated biomarkers, only 13 showed improvement in prediction performance. Results of pooled meta-analyses, non-pooled analyses, and assessments of improvement in prediction performance and risk of bias, yielded the highest predictive utility for N-terminal pro b-type natriuretic peptide (NT-proBNP) (high-evidence), troponin-T (TnT) (moderate-evidence), triglyceride-glucose (TyG) index (moderate-evidence), Genetic Risk Score for Coronary Heart Disease (GRS-CHD) (moderate-evidence); moderate predictive utility for coronary computed tomography angiography (low-evidence), single-photon emission computed tomography (low-evidence), pulse wave velocity (moderate-evidence); and low predictive utility for C-reactive protein (moderate-evidence), coronary artery calcium score (low-evidence), galectin-3 (low-evidence), troponin-I (low-evidence), carotid plaque (low-evidence), and growth differentiation factor-15 (low-evidence). Risk scores showed modest discrimination on internal validation, with lower performance on external validation.
Conclusions:
Despite high interest in this topic, very few studies conducted rigorous analyses to demonstrate incremental predictive utility beyond established CVD risk factors for T2D. The most promising markers identified were NT-proBNP, TnT, TyG and GRS-CHD, with the highest strength of evidence for NT-proBNP. Further research is needed to determine their clinical utility in risk stratification and management of CVD in T2D.
Significance of micro-EGFR T790M mutations on EGFR-tyrosine kinase inhibitor efficacy in non-small cell lung cancer: The WJOG 13119L study
Authors: Masuda Takeshi , Miura Satoru , Sato Yuki , Tachihara Motoko , Bessho Akihiro , Nakamura Atsushi , Miyawaki Taichi , Yoshimine Kohei , Mori Masahide , Shiraishi Hideaki , Hamai Kosuke , Haratani Koji , Maeda Sumiko , Tabata Eriko , Kitagawa Chiyoe , Tanizaki Junko , Imai Takumi , Nogami Shouhei , Yamamoto Nobuyuki , Nakagawa Kazuhiko , Hattori Noboru 
Published: May 2023

Abstract
Small amounts of epidermal growth factor receptor (EGFR) T790M mutation (micro-T790M), which is detected using droplet digital PCR (ddPCR) but not conventional PCR, in formalin-fixed and paraffin-embedded (FFPE) samples have been investigated as a predictive factor for the efficacy of EGFR-tyrosine kinase inhibitors (TKIs). However, the predictive value of micro-T790M remains controversial, possibly owing to the failure to examine artificial T790M in FFPE specimens. Therefore, we examined the predictive value of micro-T790M in first-generation (1G), second-generation (2G), and third-generation (3G) EGFR-TKI efficacy using a new method to exclude FFPE-derived artificial mutations in our retrospective cohort. The primary objective was time to treatment failure (TTF) of 1G, 2G, and 3G EGFR-TKIs according to micro-T790M status. In total, 315 patients with EGFR-positive NSCLC treated with 1G, 2G, and 3G EGFR-TKIs were included in this study. The proportion of micro-T790M-positive patients in the 1G, 2G, and 3G EGFR-TKI groups was 48.2%, 47.1%, and 47.6%, respectively. In the micro-T790M-positive group, the TTF was significantly longer in the 2G and 3G EGFR-TKI groups than in the 1G TKI group. No differences in the micro-T790M-negative group were observed. Micro-T790M status detected using ddPCR, eliminating false positives, may be a valuable predictor of EGFR-TKI efficacy.
Appraisal of skeletal maturity indicators with respect to non invasive biomarkers
Authors: Kumar Snigdha , Kumar Swatantra , Singh Prof (Dr.) Gyan Prakash , Saxena Prof (Dr.) Shailendra K , Shastri Prof. (Dr.) Dipti , Singh Prof (Dr.) Gulshan Kumar 
Published: May 2023

Abstract
Background
An orthodontist aims to begin treatment during pubertal growth spurt which is a promising time in terms of treatment outcomes. Salivary non-invasive biomarkers are being studied as they could be a help in predicting skeletal age assessment. This study is conducted to assess the correlation amongst salivary Insulin-Like Growth factor-1, salivary Alkaline Phosphatase, cervical vertebrae maturation stages and chronological age in the assessment of skeletal age during the growth period. This study also aims on finding associations with mandibular base length and maxillary base length.
Methods
A number of 80 suitable subjects from 7 to 21 years of age were selected and divided into two groups based on the sex (41 males, 39 females). They were further sub-grouped on the basis of six cervical vertebrae maturation stages from the lateral cephalogram. For every subject chronological age was reported, maxillary base length and mandibular base length was calculated from tracing on lateral cephalogram. Saliva specimens were retrieved from each subject to evaluate the salivary IGF-1and ALP levels by using the enzyme-linked immunosorbent assay (ELISA). The retrieved data was subjected to statistical analysis in SPSS software (20.0).
Results
The mean salivary IGF-1 activity were found to be at peak in CVMI stage 3 and highest mean salivary ALP levels were found to be in CVMI stage 3 in males and CVMI stage 4 in females.
Conclusions
The mean salivary ALP and salivary IGF-1 levels are well correlated with CVMI stages. Salivary ALP levels show correlation with chronological age and could also be used as a diagnostic model to predict cervical stage.
Functional improvement of unicompartmental knee arthroplasty compared with total knee arthroplasty for subchondral insufficiency fracture of the knee
Authors: Suh Dae Keun , Suh Dong Won , Park Jun-Gu , Han Seong-Beom , Kim Jaejoong 
Published: May 2023

Abstract
Background
Subchondral insufficiency fracture of the knee (SIFK) causes acute knee pain in adults and often requires surgical management. Unicompartmental knee arthroplasty (UKA) and total knee arthroplasty (TKA) are the two most common surgical treatments for SIFK. While both UKA and TKA have their advantages, there is no consensus for SIFK localized on the medial compartment. We hypothesized that patients with SIFK treated with UKA would show superior patient-reported outcomes compared to those who underwent TKA.
Methods
A total of 90 patients with SIFK were included in the TKA (n = 45) and UKA (n = 45) groups, respectively. SIFK lesions were measured on MR images. Hip knee ankle (HKA) angle was obtained preoperatively and at the final follow-up. Patient-reported outcomes in the form of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Hospital Special Surgery (HSS) scores, and Knee Society Scores (KSS) were assessed preoperatively, post-operative 6, 12 months, and at the final follow-up.
Results
The UKA group had better range of motion of the knee preoperatively and postoperatively than the TKA group (p<.01 and p<.01). At 6 months, WOMAC score was better in the UKA group than the TKA group (p<.01). Both groups had a significant improvement in WOMAC, HSS, and KSS scores at the final follow-up compared to preoperative scores. There were no surgical complications in the TKA group, but the UKA group had 3 revision cases due to a bearing failure.
Conclusion
Unicompartmental arthroplasty and total joint arthroplasty can produce successful outcomes in patients with SIFK with proper patient selection, regardless of the size of SIFK lesion.
A Nomogram Model Based on the Risk of Lymph Node Metastasis to Evaluate the Prognosis of Gastric Cancer
Authors: 覃 语思 , Xia Yiju , Liu Li , Chen Yao , Xiao Bin , Feng Xiaofeng , Wu Hongbo , Ran Fan , Dai Jianhua , Peng Guiyong 
Published: May 2023

Abstract
Background: Gastric cancer is one of the most common malignancies that pose a serious risk to human health worldwide. Lymph node metastasis may serve as an important prognostic factor for gastric cancer. However, the use of lymph nodes as a prognostic indicator for gastric cancer may lead to stage migration. The aim of this study is to establish a nomogram model to increase the prognostic accuracy of gastric cancer by validating the prognostic role of lymph node metastatic risk.
Methods: A total of 3716 patients with gastric cancer were included in this study based on information from the SEER database. The risk factors for lymph node metastasis were analyzed correlatively. Prognostic factors were analyzed by COX regression, on which nomograms were constructed. The model was validated with ROC and C-indices, and Kaplan-Meier curves were evaluated and analyzed for each prognostic factor.
Results: Age, sex, T-stage, N-stage, M-stage, tumor size and lymph node ratios all served as independent prognostic factors for gastric cancer. lymph node metastasis ratio is a prognostic factor in gastric cancer that is superior to lymph node number. The nomogram model has 3- and 5-year AUC values of 0.797 and 0.817 respectively, with a C-index of 0.730.
Conclusion: As a prognostic factor of gastric cancer, lymph node metastasis ratio can reduce stage migration, and its inclusion in the nomogram model is superior to the TNM staging system to evaluate the prognosis of gastric cancer. The nomogram model we have developed has high reliability and accuracy and thus can inform personalized clinical treatment for gastric cancer patients.
Neural Network-Based Soil Parameters Predictive Coordination Algorithm for Energy Efficient Wireless Sensor Network
Authors: Sharma Dinesh , Tomar Geetam Singh 
Published: May 2023

Abstract
Wireless Sensor Network (WSN) is the best implementation of Information Technology in the agricultural sector. With interest blooming in them, and evolving technology, sensor networks are not only capable of giving the real-time nutrient index of the soil, but could also help to regulate all agricultural processes. However, WSN comes with a major limitation, i.e., energy. Based on experimental data collected from various soil forms, and analyzed using advanced data analytics, the Nutrient Index is somehow linked and doesn’t show frequent change with time. Therefore, by using predictive neural network techniques, one can easily get detailed info with primary inputs received from WSN. There is no need to make the WSN run for the entire day. Instead, for making an energy-efficient WSN, NR-MDEC protocol is used along with a coordination algorithm which enhances the efficiency dramatically.
lesSDRF Is More: Maximizing The Value Of Proteomics Data Through Streamlined Metadata Annotation
Authors: Claeys Tine , Bossche Tim Van Den , Perez-Riverol Yasset , Gevaert Kris , Vizcaino Juan Antonio , Martens Lennart 
Published: May 2023

Abstract
Sharing data and resources has revolutionized life sciences, particularly in proteomics, where public data has enabled researchers to reanalyze and reinterpret data in novel ways. However, the lack of comprehensive metadata remains a significant challenge to unlocking the full potential of publicly shared data. In response, the Sample and Data Relationship Format (SDRF) Proteomics was developed, However, its complexity presents several challenges. This study investigated metadata annotations in proteomics data sets from the PRIDE database and the corresponding publications, and identified major gaps in metadata provision. To bridge this gap, we created a user-friendly, ontology-based Streamlit application, named lesSDRF, that guides users through the annotation process using SDRF. lesSDRF aims to encourage researchers to provide more detailed metadata annotations, leading to greater insights and scientific advances in proteomics. By addressing this issue, we can facilitate more collaborative efforts and enhance our understanding of biological processes. LesSDRF is available via https://compomics-lessdrf-home-2rdf84.streamlit.app/.
High peritoneal incision approach in endoscopic transabdominal preperitoneal patch plasty (TAPP) for inguinal hernia after radical prostatectomy
Authors: Yamamoto Tetsu , Hyakudomi Ryoji , Takai Kiyoe , Uchida Yuki , Ishitobi Kazunari , Hirahara Noriyuki , Tajima Yoshitsugu 
Published: May 2023

Abstract

Background: Inguinal hernia develops as one of the common complications after robotic or laparoscopic radical prostatectomy (RP). TAPP for an inguinal hernia after RP is far from easy to perform due to postoperative severe adhesions in the preperitoneal cavity. We recently devised a high peritoneal incision approach (HPIA) in TAPP for inguinal hernia patients in whom peritoneal dissection is difficult due to severe adhesions after RP. We evaluate the safety and efficacy of TAPP with a high peritoneal incision approach for patients with an inguinal hernia after Robot-assisted radical prostatectomy.
Methods Patients characteristics and surgical outcome were evaluated by a retrospective analysis.
Result: From January 2014 to December 2017, 21 consecutive patients underwent TAPP for an inguinal hernia after RARP. Twenty-four lesions were the Type 3b and 3 were Type 3a according to the Nyhus classification. A conventional TAPP was performed for 10 hernia lesions in 8 patients and TAPP with HPIA was utilized for 17 lesions in 13 patients. The mean operation time for the unilateral hernia in the HPIA (137.8±20.7 min) was significantly shorter than that (182.2±42.0 min) in the conventional TAPP (p =0.038). The HPIA was complete in all patients, while the conventional TAPP was converted to IPOM in 5 patients (55.6%, p=0.008) due to dense adhesions with difficult dissection. No recurrent was observed after follow-up period of 24months in both groups.
Conclusions: The TAPP with HPIA is feasible and a safe and reliable treatment of choice in patients with an inguinal hernia after RARP.
Sense of place and post-traumatic growth in the post-COVID era: A moderated mediation model of psychological resilience and social-emotional competence
Authors: Zhang Jianzhen , Chen Yifei , Yu Hongmei , An Zhenni , Antwi Collins Opoku , Ge Jiahao 
Published: May 2023

Abstract

Background In the post-COVID era, identifying ways to promote post-traumatic growth among the general public is a pressing concern. However, few studies have explored the potential positive impact of people’s relationship with place on post-traumatic growth. Therefore, this study investigated the relationship between sense of place) and post-traumatic growth, as well as the mediating role of psychological resilience and moderating effect of social-emotional competence. Methods We conducted a survey with 1203 high school students in eastern China to assess their levels of sense of place, post-traumatic growth, psychological resilience, and social-emotional competence. Moderated mediation models were tested using SPSS, AMOS, and PROCESS. Results The results indicated a significant positive impact of sense of place on post-traumatic growth (β = 0.111, p < 0.001), with psychological resilience serving as a mediator (β = 0.401, p < 0.001). Moreover, social-emotional competence moderated the relationship between sense of place and psychological resilience in this pathway (β = 0.073, p < 0.001). Conclusions The results indicated that sense of place may have both direct and indirect impacts through psychological resilience on post-traumatic growth. In addition, individuals with higher social-emotional competence tend to experience more post-traumatic growth, even when their sense of place is similar to individuals with lower social-emotional competence. These findings have significant implications for promoting post-traumatic growth among adolescents in the post-COVID era.
Identifying the causes of the high incidence of cholelithiasis and a health economics evaluation of two types of cholecystectomies in Qinghai province
Authors: Xia Yonghong , Jing# Xiaofeng , Ma# Ying , Li# Defu , Zhang Tiecheng , Xiang Haiqi , Xu Fan 
Published: May 2023

AbstractBackground:Qinghai province has a high incidence of cholelithiasis. However, the risk factors for cholelithiasis in a high-altitude area remain unclear. It is urgent to formulate and promote the diagnosis and treatment norms of this disease.Objective:We explored the causes of the high incidence of cholelithiasis in a high-altitude area to assess the clinical and economical differences between open cholecystectomy (OC) and laparoscopic cholecystectomy (LC), two surgical approached to treat cholelithiasis, and to establish a risk prediction model.Methods:In this retrospective study, we enrolled 8,596 patients to explore the spatiotemporal distribution of the disease. We ultimately selected 3,914 treated with cholecystectomy: 64 received OC and 3,850 received LC. We evaluated differences in clinical factors and health economics between the groups by using Student’s t-test and the chi-square test. We used multivariate analysis of variance and step regression to identify the risk factors of cholelithiasis.Results:There were significantly fewer cases in spring than in winter (p= 0.001). There were significant differences in age, surgical expenses, total expenses, and length of stay between the OC and LC groups (p= 0.001). The step regression model revealed that the risk factors for cholelithiasis are the surgical approach, age, sex and the outcome after hospital discharge.Conclusion:In Qinghai province, the incidence of cholelithiasis was higher in winter. Compared with OC, LC showed better performance from both the clinical and economical points of views. Besides, the prediction model demonstrated that the surgical approach, sex, age and the outcome after discharge affect the prognosis for patients with cholelithiasis.
The Impact of Eosinophil Cationic Protein on Nasal Epithelial Cells: An Investigation of Cellular Responses
Authors: Hou Jing , zhang luo 
Published: May 2023

Abstract

Introduction
The impact of eosinophil cationic protein (ECP) on nasal epithelial cells remains unclear. Our study investigated whether ECP could activate nasal epithelial cells to secrete interleukin 8 (IL-8).
Materials and Methods
The airway epithelial cells, NCI-H292, were cultured separately and exposed to varying concentrations of ECP for 2, 4, 6, 16, or 24 hours. The supernatant was collected and IL-8 was tected using ELISA.
Results
The results showed a significant increase in IL-8 levels in the ECP group compared to the control group at 24 hours (50.92ug/ml±20.06% versus 1.95ug/ml).
Conclusion
The study concludes that ECP stimulates H292 cells to secrete high levels of IL-8, causing damage to epithelial cells. The increase in ECP in allergic disease can aggravate inflammation of the airway mucosa.
Minor effects of no-till treatment on GHG emissions of boreal cultivated peat soil
Authors: Honkanen Henri , Kekkonen Hanna , Heikkinen Jaakko , Lång Kristiina 
Published: May 2023

Abstract
Greenhouse gas emissions of a spring cereal monoculture under conventional tillage and no-till treatments were measured in a peatland in Southwestern Finland for three years in 2018–2021. Nitrous oxide (N2O), carbon dioxide (CO2) and methane (CH4) fluxes were measured with an opaque chamber technique approximately biweekly throughout the years. During the growing season, canopy net ecosystem exchange (NEE) was measured with a transparent chamber technique and hourly ecosystem respiration (ER) and gross photosynthesis (GP) were modelled with empiric models. On average, the annual emissions were 6.4 ± 2.4 Mg CO2-C ha − 1 yr− 1, 7.6 ± 3.5 kg N2O -N ha− 1 yr− 1, and − 0.35 ± 0.42 kg CH4-C ha− 1 yr− 1 for NEE, N2O and CH4, respectively. The effect of no-till management on the GHG balance was non-consistent through years and thus generally of minor significance. No-till reduced the annual CO2 emissions by 24% in 2019 and N2O emissions by 33% in 2020 compared to conventional tillage while there were no differences between the treatments in other years. Measured differences in ER occurred mostly during the winter periods, especially after ploughing. The results indicated that no-till may reduce CO2 and N2O emissions from cultivated peat soil, but it does not lead to large consistent reductions during the first years of NT management.
Whole-field density measurements by Digital Image Correlation
Authors: Oers Alexander M. van , de Kat Roeland , Maas Leo R.M. 
Published: May 2023

Abstract
A new optical method is applied for the quantitative measurement of the density field of two-dimensional, stratified or homogeneous, transparent fluids in a laboratory set-up. A crucial new step is the angle at which we place a camera to view the experimental set-up. This step is motivated by a fallacy observed when applying our method in a classical configuration, in which the camera's optical axis is perpendicular to the flat surface of a fluid container. Application of this method is illustrated by the optical determination of static density fields of linearly and nonlinearly stratified fluids, as well as of multi-layered fluids. The method is validated by comparing with density profiles obtained from probe measurements of conductivity and temperature. Our method yields similar density and buoyancy profiles as the probe while also providing a whole field measurement, without disturbing the fluid and allowing the determination of dynamical density fields.