bluuebunny/crossref_metadata_2025_split huggingface dataset:
The morphofunctional implications of the glenoid labrum of the glenohumeral joint in hominoids
Authors: Raventós‐Izard Georgina , Potau Josep Mª. , Casado Aroa , Pastor Juan F. , Arias‐Martorell Julia 
Published: March 2023

AbstractObjectivesA morphocline of the glenoid cavity has been used to infer differences in locomotor behaviors; however, the glenoid cavity is surrounded by the glenoid labrum, a fibrocartilaginous structure that could influence the functionality of the glenoid. The objectives of this study are to explore the effects of the glenoid labrum on the area, depth, and morphology of the glenoid cavity in primates.Materials and MethodsPhotogrammetry was used to build 3D models of the glenoid, with and without the labrum, and three‐ (3D) and two‐dimensional (2D) geometric morphometrics (GM) was applied. 2D areas were collected from zenithal images for glenoids with and without labrum to evaluate the availability of articular surface area.ResultsIn the 2D GM the morphocline is present in the dry‐bone sample but not with the presence of the glenoid labrum. In the 3D GM there are differences between species mainly concerning the depth of the glenoid cavity. 2D areas reveal that the amount of articular area of the glenoid cavity increases with the presence of the labrum, particularly in humans.DiscussionThe glenoid labrum changes the shape, increases the depth and the surface area of the glenoid cavity, particularly in humans. Therefore, the glenoid labrum might hold a functional role, increasing the stability of the glenohumeral joint of primates in general, and especially in humans.
Vinyl Halide‐Modified Unsaturated Cyclitols are Mechanism‐Based Glycosidase Inhibitors
Authors: Danby Phillip M. , Jeong Andrew , Sim Lyann , Sweeney Ryan P. , Wardman Jacob F. , Karimi Ryan , Geissner Andreas , Worrall Liam J. , Reid Jolene. P. , Strynadka Natalie C. J. , Withers Stephen G. 
Published: March 2023

AbstractSuitably configured allyl ethers of unsaturated cyclitols act as substrates of β‐glycosidases, reacting via allylic cation transition states. Incorporation of halogens at the vinylic position of these carbasugars, along with an activated leaving group, generates potent inactivators of β‐glycosidases. Enzymatic turnover of these halogenated cyclitols (F, Cl, Br) displayed a counter‐intuitive trend wherein the most electronegative substituents yielded the most labile pseudo‐glycosidic linkages. Structures of complexes with the Sulfolobus β‐glucosidase revealed similar enzyme‐ligand interactions to those seen in complexes with a 2‐fluorosugar inhibitor, the lone exception being displacement of tyrosine 322 from the active site by the halogen. Mutation of Y322 to Y322F largely abolished glycosidase activity, consistent with lost interactions at O5, but minimally affected (7‐fold) rates of carbasugar hydrolysis, yielding a more selective enzyme for unsaturated cyclitol ether hydrolysis.
Verwendung der Struktur und Dynamik der Squalen‐Hopen‐Zyklase für die (−)‐Ambroxid Produktion**
Authors: Schneider Andreas , Curado Christian , Lystbaek Thomas B. , Osuna Sílvia , Hauer Bernhard 
Published: March 2023

AbstractTerpen‐Zyklasen bergen enormes synthetisches Potenzial, da sie in der Lage sind, komplexe Kohlenwasserstoffgerüste aus achiralen Ausgangsstoffen in einer einzigen kationischen Umlagerungskaskade zu generieren. Dieses synthetische Potenzial nutzbar zu machen, erwies sich jedoch aufgrund ihrer allgemein geringen katalytischen Leistung als schwierig. In dieser Studie enthüllen wir das katalytische Potenzial der Squalen‐Hopen‐Zyklase (SHC), indem wir uns ihre Proteinstruktur und Dynamik zunutze machen. Zunächst haben wir das aktive Zentrum und den Eingangstunnel des Enzyms synergistisch evolviert, um damit eine 397‐fach verbesserte (−)‐Ambroxid‐Synthase zu erzeugen. Unsere computergestützten Untersuchungen erklären, wie die eingeführten Mutationen zusammenarbeiten, um die Substrataufnahme, den Fluss und die Vorfaltung zu verbessern. Kinetische Untersuchungen zeigten jedoch eine Terpen‐induzierte Inaktivierung der membrangebundenen SHC als Hauptlimitierung für die Biokatalyse in vivo. Indem diese Erkenntnisse mit der verbesserten und stereoselektiven Katalyse des Enzyms kombinierten wurden, konnte eine Fütterungsstrategie angewendet werden, um 105 katalytische Umsätze zu übertreffen. Diese Ergebnisse haben das Potenzial die Lücke für eine breitere Anwendung von SHCs in der synthetischen Chemie zu schließen.
Entschlüsselung des Fucose‐Migrationsproduktes bei der Massenspektrometrischen Analyse von Blutgruppenepitopen
Authors: Lettow Maike , Greis Kim , Mucha Eike , Lambeth Tyler R. , Yaman Murat , Kontodimas Vasilis , Manz Christian , Hoffmann Waldemar , Meijer Gerard , Julian Ryan R. , von Helden Gert , Marianski Mateusz , Pagel Kevin 
Published: March 2023

AbstractFucose ist ein signalgebendes Kohlenhydrat, das am Ende der Glykosylierung angehängt wird. Es ist an einer Reihe von Prozessen beteiligt, z. B. an der Selectin‐abhängigen Leukozytenadhäsion oder an Pathogen‐Rezeptor‐Interaktionen. Massenspektrometrische Verfahren, die üblicherweise zur Bestimmung der Struktur von Glykanen eingesetzt werden, zeigen häufig Fucose‐haltige, chimäre Fragmente, die die Analyse verzerren. Die Umlagerung, die zu diesen Fragmenten führt – oft als Fucose‐Migration bezeichnet – ist seit mehr als 25 Jahren bekannt, aber die chemische Identität des Umlagerungsproduktes bleibt unklar. In dieser Arbeit kombinieren wir Ionenmobilitätsspektrometrie, Massenspektrometrie mit radikalinduzierter Dissoziation, kryogene IR‐Spektroskopie und Computersimulationen mittels Dichtefunktionaltheorie, um das Produkt der Umlagerung der prototypischen Trisaccharide Lewis x und Blutgruppe H2 zu bestimmen. Die Struktursuche ergibt, dass die Fucose, die mit einer α(1→6)‐glykosidischen Bindung an die Galaktose gebunden ist, das wahrscheinlichste Produkt ist.
An Artificial In Vitro Metabolism to Angiopterlactone B Inspired by Traditional Retrosynthesis**
Authors: Kiefer Alexander F. , Liu Yu‐Chang , Gummerer Rebecca , Jäger Christina , Deska Jan 
Published: March 2023

AbstractNature's way to construct highly complex molecular entities as part of biosynthetic pathways is unmatched by any chemical synthesis. Yet, relying on a cascade of native enzymatic transformations to achieve a certain target structure, biosynthesis is also significantly limited in its scope. In this study, non‐natural biocatalytic modules, a peroxidase‐mediated Achmatowicz rearrangement and a dehydrogenase‐catalyzed borrowing‐hydrogen‐type isomerization were successfully incorporated into an artificial metabolism, combining the benefits of traditional retrosynthesis with the elegance and efficacy of biosynthetic networks. In a highly streamlined process, the total synthesis of tricyclic angiopterlactone B was achieved in two steps operating entirely in an aqueous environment while relying mainly on enzymes as key reaction mediators.
The influence of the Milan approach: Five decades of intergenerational change. A conversation with Matteo Selvini
Authors: Amorin‐Woods Deisy , Selvini Matteo 
Published: March 2023

AbstractThe ‘Milan Approach’ made an enormous contribution to psychotherapy and psychiatry and has been viewed as a bedrock in the family therapy field. Mara Selvini‐Palazzoli made a revolutionary shift in the early 1970’s to abandon individual therapy and adopt family therapy to treat anorexia in young women. The goal of the three systemic principles; hypothesising, circularity and neutrality, proposed by the Milan Team is to elicit a useful method to interview families which encourages the production of meaningful information.The Milan group took special care of language. Rather than overly focusing on the label and referring to schizophrenia as a diagnosis, for instance, they used the term ‘families in schizophrenic transaction’. This perspective more realistically explored the origin of problems while looking at ways how to effectively generate change. In the early stages of its development, heavily influenced by the work of the MRI, the team adopted a pragmatic and strategic approach, however the focus gradually shifted towards semantics and the story of the system. While the ‘systemic identity’ was the principal guiding principle, the model was also influenced by social constructionism.This paper is a distillation of a conversation held with Matteo Selvini, son of Mara Selvini Palazzoli. We discussed the four key influential figures who developed the ‘Milan approach’, Selvini Palazzoli, Boscolo, Cecchin and Prata. The focus of the conversation is on Mara and on her influence shaping the approach, its evolution through the years leading to the creation of the Scuola di Psicoterapia Mara Selvini Palazzoli.Matteo also shares his own influence in the evolution of the school through the intervening years. He explains the adapted version of the Milan approach and the amalgam of the original and emerging therapeutic concepts. His key messages to therapists are the importance of working in teams and client follow‐ups.
Effects of compositional parameters on morphology and rheological properties of HDPE/PVA blends
Authors: de Melo Carvalho Benjamim , da Silva Jorge Manuel Jardim , Farah Marcelo , Kaipers Stocker Mariele , Lauer Sanson Murilo 
Published: March 2023

AbstractPolymeric blends are relevant for research and industry because of their versatility, being able to accomplish property tailoring with competitive cost and ease to scale up, when compared to the development of in‐reactor new polymers. Blends of thermoplastics, however, might have their morphology and properties altered upon processing, therefore, there is uncertainty to some outputs after melt processing. Therefore, having a fixed morphology that is capable of being further processed without significant changes have industrial and academic value. This work presents a comprehensive study of rheological properties and morphology of a selectively crosslinked blend of polyethylene (HDPE) and poly(vinyl alcohol) (PVA), where the effect of the following parameters are evaluated: dispersed phase (PVA) weight fraction, continuous phase (HDPE) rheological properties, compatibilizer (PE‐g‐MAh) and crosslinking agent (maleic anhydride) content. Overall, melt complex viscosity and elasticity have increased with PVA weight fraction and HDPE viscosity. The compatibilizer content has not severely affected melt rheology, although it influenced blend morphology. Crosslinking agent content affected melt rheology and glass transition temperature in a complex way due to the probable competition of some phenomena (crosslinking and reaction vs reduction of crystallinity degree and H bond attenuation), with little effect over PVA morphology.
Air‐based polyethylene fragmentation with high yield to form microplastic particles as reference material candidates
Authors: Schmitt Marco , Altmann Korinna , Fengler Petra , Gehde Michael 
Published: March 2023

AbstractMicroplastic (MP) particles with sizes between 1 and 1000 µm are widely distributed worldwide. Origin, transport pathways, and fate are poorly known, as sampling, sample preparation, and detection methods are major challenges. In addition, reference materials that mimic environmental particles are lacking. The most challenging is the yield of MP particle production and the need for resource‐intensive grinding with liquid nitrogen. In this paper, a machine is designed to produce aged microplastic particles as reference material candidates with high yield. The machine is based on ultraviolet aging of a thin foil and mechanical fragmentation using clean air. An example of aging and fragmentation of high‐density polyethylene with additional physical and chemical characterization of shape, size, aging state by carbonyl index, and density is presented.
Graphene‐Encapsulated Nitrogen‐doped Carbon@Bi Enables Rapid, Ultrahigh and Durable Sodium Storage
Authors: Wang Meixue , Li Huijun , Cheng Xiaoqin , Tian Shizhen , Wang Xiaomin 
Published: March 2023

AbstractBismuth (Bi) has promising applications as anode in sodium‐ion batteries (SIBs) due to its suitable alloy voltage, unique lattice structure and high volumetric specific capacity. However, the severe structural degradation and poor cycling performance of Bi‐anode during cycling limits its practical application. Here, we designed and synthesized graphene‐encapsulated nitrogen‐doped carbon@bismuth (N−C@Bi/G) composites as anode materials for SIBs by a two‐step hydrothermal and thermal treatment method to solve the above problems. The N−C@Bi/G composite anode material exhibits excellent sodium storage performance, high capacity (280 mAh g−1 at 0.1 A g−1 and an initial coulombic efficiency of 78.87 %) and long‐term cycling stability (260 mAh g−1 after 1000 cycles at 2 A g−1). Electrochemical analysis and DFT calculations show that the fast reaction kinetics of N−C@Bi/G is directly related to the introduction of nitrogen‐doped carbon enriching the pore structure and increasing the active sites. Meanwhile, through the aid of in‐situ X‐ray diffraction (XRD), the reaction mechanism and interphase evolution of N−C@Bi/G composite are revealed, which provides an important theoretical basis for the application of SIBs.
Potential and Limitations of Research Battery Cell Types for Electrochemical Data Acquisition
Authors: Smith Anna , Stüble Pirmin , Leuthner Lea , Hofmann Andreas , Jeschull Fabian , Mereacre Liuda 
Published: March 2023

AbstractDeveloping new electrode materials and/or electrolytes for lithium‐ion batteries requires reliable electrochemical testing thereof. For this purpose, in academic research typically hand‐made coin‐type cells are assembled. Their advantage is a rather cheap and facile assembly, and possibility to prepare full‐cells as well as half‐cells, meaning cathode‐anode or electrode‐elemental lithium configurations. Critical parameters for testing data quality and the potential and limitations of cell tests in half‐cell configuration are discussed. Further, on the basis of a round robin test, using highly homogenous commercial electrodes, where graphite is used as anode and LiNi0.33Mn0.33Co0.33O2 (NMC111) as the cathode material, it is shown that data acquired is highly influenced by assembling parameters. Besides known variables such as the amount of electrolyte or electrode positioning, the proper height of the cell stack and the steel grade of the housing material are identified as decisive variables. Finally, it is demonstrated that under proper conditions coin cells can show a great cycle stability of >2200 cycles using 1 C as dis‐/charge rate while retaining a capacity of 80 %. This performance is close to pouch‐type cells containing the same electrodes and electrolyte, which were used as a benchmark system and showed >3500 cycles of lifetime.
Simultaneous positive and negative selection of proteases in bacterium based on cell suicide and antibiotic resistance
Authors: Yekeen Abeeb Abiodun , Zhang Lu , Liu Haiyan , Chen Quan 
Published: March 2023

AbstractMethods for engineering protease specificity and selectivity toward target substrates of therapeutic or industrial relevance are of wide interest. Herein, we report a bacterial system for the simultaneous detection and selection of protease activity on positive and negative target substrates. The system, based on Proteolysis‐triggered bActerial SuiCide and Antibiotic Resistance (PASCAR) in Escherichia coli, exploits the β‐lactamase and the human gasdermin D (GS) for the proteolysis‐responsive positive and negative selections, respectively. The applicability of the positive selection was illustrated with the directed evolution of the Tobacco etch virus protease toward the recognition of non‐native substrates. We also utilized the positive selection for the efficient evaluation of computationally redesigned protease variants. We constructed and optimized a series of GS mutants as suicide modules – with high to low selection stringencies – that would enable the use of PASCAR as a practically applicable dual selection system. This study provides a simple and easily accessible tool that facilitates the engineering of proteases with custom specificity and selectivity.
Structured instruments for insanity defense evaluations: Opportunities and limitations
Authors: Parmigiani Giovanna , Mandarelli Gabriele , Meynen Gerben , Ferracuti Stefano 
Published: March 2023

AbstractInsanity evaluations are often criticized for their—alleged—lack of objectivity, reliability and transparency. Structured tools to guide and support forensic evaluators during these evaluations have been developed—but they are rarely employed in forensic practice. In the present article, we consider the value of these tools for forensic practice in terms of opportunities and limitations. First, we briefly describe different insanity criteria used in Western countries. Next, we will review five structured instruments to guide insanity assessment together with their performance measures. Finally, we draw conclusions on the value of such instruments for forensic practice.
Ego depletion in consumer behavior: A review, synthesis, and research agenda
Authors: Rafael Diego Nogueira , Lopes Evandro Luiz 
Published: March 2023

AbstractThis research aims to identify, analyze, and map the intellectual structure of the topic of ego depletion in consumer behavior through the bibliometric strategies of namely co‐citation analysis and bibliographic coupling. We performed the co‐citation analysis covering 20 years of research, divided into two 10‐year analyses. The bibliographic coupling analysis focused on recent research covering the last decade, divided into two five‐year analyses. The results presented the most important research, authors, research centers, and journals on the topic, as well as the factors derived from the co‐citation and bibliographic coupling analysis, the evolution of the theme between the analysis and subsequent periods, and the agenda for future research. The main contribution of this article is the proposition of an agenda for future research on ego depletion and its effects on consumer behavior.
Carbon Quantum Dots as Multi‐Purpose Nanomaterial in Stem Cell Therapy
Authors: Bahrampour Juybari Kobra , Rizwan Komal , Faramarz Sanaz , Sadeghi Asie , Amirkhosravi Arian , Hadi Nematollahi Mohammad , Mehrabani Mehrnaz 
Published: March 2023

AbstractDuring stem cell therapy, some issues, such as obscure fate of stem cells or their low survival rate in the body, should be addressed to boost their therapeutic efficiency. Nanotechnology offers a suitable solution to combat such limitations. Carbon quantum dots (CQDs) are carbon‐based nanomaterials and may be used as multi‐purpose compounds in stem cell therapy. CQDs are excellent choices for stem cell labeling thanks to their special features such as optical properties and good biocompatibility. Besides, they can modulate the biological function of stem cells, such as their proliferation, homing ability, and differentiation properties. Considering the charismatic feature of CQDs and their broad unique effect on stem cells, the current review aims to summarize the most advancements in this field. Hence, we first focused on CQDs synthesis and their applications. In the next section, the stem cell categories will be discussed, and the final part is dedicated to the recent research evaluating the impact of CQDs on stem cell therapy.
Reversible Photocontrol of Microtubule Stability by Spiropyran‐Conjugated Tau‐Derived Peptides
Authors: Inaba Hiroshi , Sakaguchi Minamo , Watari Soei , Ogawa Shigesaburo , Kabir Arif Md. Rashedul , Kakugo Akira , Sada Kazuki , Matsuura Kazunori 
Published: March 2023

AbstractSpatiotemporal modulation of microtubules by light has become an important aspect of the biological and nanotechnological applications of microtubules. We previously developed a Tau‐derived peptide as a binding unit to the inside of microtubules. Here, we conjugated the Tau‐derived peptide to spiropyran, which is reversibly converted to merocyanine by light, as a reversible photocontrol system to stabilize microtubules. Among the synthesized peptides with spiropyran/merocyanine at different positions, several peptides were bound to the inside of microtubules and stabilized the structures of microtubules. The peptide with spiropyran at the N‐terminus induced polymerization and stabilization of microtubules, whereas the same peptide with the merocyanine form did not exert these effects. Reversible formation of microtubules/tubulin aggregates was achieved using the peptide with spiropyran conjugated at the N‐terminus and irradiation with UV and visible light. Spiropyran‐conjugated Tau‐derived peptides would be useful for spatiotemporal modulation of microtubule stability through reversible photocontrol of binding.
Bilateral breast Rosai‐Dorfman disease screen detected by mammography
Authors: Sumner Christina , Salem Karma , Abunimer Luma , Ewaz Abdulwahab , Zhang Linsheng , Monsrud Ashley , Calisi Nabil 
Published: March 2023

AbstractRosai‐Dorfman disease (RDD) is a proliferative disorder of histiocytes typically found in nodal sites and commonly observed in females. Patients often present with systemic symptoms such as fever, lymphadenopathy, and weight loss. However, extra‐nodal disease has been identified in locations including the skin and subcutaneous tissue. We present a case of a 59‐year‐old female presenting with abnormal bilateral findings on screening mammography, who was found to have a rare presentation of Rosai‐Dorfman disease.
Postprandial hypoglycemia after ileocolic interposition and Billroth‐II gastrojejunostomy: A case report
Authors: Unhapipatpong Chanita , Hiranyatheb Pitichote , Phanachet Pariya , Warodomwichit Daruneewan , Sriphrapradang Chutintorn , Shantavasinkul Prapimporn Chattranukulchai 
Published: March 2023

AbstractPostprandial reactive hypoglycemia, or late dumping syndrome, is a common but underrecognized complication from bypass surgery. We report an unusual case of postprandial reactive hypoglycemia in a patient with a severe esophageal stricture from corrosive agent ingestion who underwent ileocolic interposition and an antecolic Billroth‐II gastrojejunostomy. A 22‐year‐old male patient with a one‐year history of corrosive ingestion was referred to the hospital for a surgical correction of severe esophageal stricture. After the patient underwent ileocolic interposition and an antecolic Billroth‐II gastrojejunostomy, he experienced multiple episodes of gastroesophageal refluxsymptoms during nasogastric feeding and had onset of hypoglycemic symptoms. His plasma glucose level was 59 mg/dL. After we had intraoperatively re‐inserted a jejunostomy tube bypassing the ileocolic interposition, and reintroduced enteral nutrition, his hypoglycemic symptoms resolved. We performed a mixed meal tolerance test by nasogastric tube, but the results did not show postprandial hypoglycemia. Although the specific mechanism is unclear, this case suggests gastroesophageal reflux to the ileal interposition may have caused a state of exaggerated hyperinsulinemic response and rebound hypoglycemia. To the best of our knowledge, we are the first to report case of postprandial hypoglycemia after ileocolic interposition, which may have been caused by exaggerated hyperinsulinemic response due to gastroesophageal reflux to the ileal interposition. This syndrome should be considered in the patient who has had ileocolic interposition surgery and has developed postprandial hypoglycemia.