bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the relationship between de‐novo DSA development and CXCR5+PD‐1+CD8+ T cells and PD‐1/PD‐L1 mRNA expression in kidney transplant recipients
Authors: Ekinci Nurten Sayin , Yilmaz Vural Taner , Kilinc Yahya , Kocak Huseyin , DARBAS Sule , Kisaoglu Abdullah , Aydinli Bulent , Uzuner Sezin Yakut , Ucar Fahri 
Published: August 2023

AbstractBackground and aimsThe relationship between the Follicular Cytotoxic T cell subgroup and expression levels of PD1/PD‐L1 genes and the development of donor specific antibody (DSA) is unknown. In this study, we aimed to examine CD8+CXCR5+PD‐1+ follicular cytotoxic T cell levels and expression levels of PD1/PD‐L1 genes in peripheral blood lymphocytes in de‐novo DSA positive and negative kidney transplant recipients (KTR).MethodsIn our study, expression of PD‐1/ PD‐L1 genes by Real‐Time Quantitative PCR method and CD8+CXCR5+PD‐1+ T cell expression levels by flow cytometric method were obtained from peripheral blood samples. 63 participants were included in the study (de‐novo DSA positive recipients (n = 22, group 1), de‐novo DSA negative recipients (n = 20, group 2) and healthy control (n = 21, group 3). All patients had negative PRA before kidney transplantation. Expression (%) levels of target cells were evaluated by flow cytometry method. IBM SPSS Statistics for Windows Version 22 and R.3.3.2 software were used to evaluate the data.ResultsThe demographic data of the groups were similar. PD‐1 mRNA expression was higher in de‐novo DSA positive KTR than negative (respectively, 1.03 ± .29/.82 ± .15, p: .001). CD8+CXCR5+PD‐1+ T cell expression levels were found to be higher in the de‐novo DSA positive group than in the negative group and similar to the healthy group (respectively, 3.06 ± 1.98/.52 ± .40, p:.001, 3.06 ± 1.98/2.78 ± .59, p:.62). The percentage of CD8+CXCR5+PD‐1+ expressing T cells was significantly lower in the HLA‐Class II+ group than other groups (HLA CI/II/ I+II, respectively, 3.63 ± 2.72/1.65 ± .50/3.68 ± 1.67, p: .04).ConclusionsIn our study, a significant relationship was found between DSA formation and PD‐1 mRNA level and CD8+CXCR5+PD‐1+ follicular cytotoxic T cell in KTR.
Study protocol: A comparison of mobile and clinic‐based spirometry for capturing the treatment effect in moderate asthma
Authors: Izmailova Elena S. , Kilian Rachel , Bakker Jessie P. , Evans Shawna , Scotina Anthony D. , Reiss Theodore F. , Singh Dave , Wagner John A. 
Published: August 2023

AbstractSeveral inefficiencies in drug development trial implementation may be improved by moving data collection from the clinic to mobile, allowing for more frequent measurements and therefore increased statistical power while aligning to a patient‐centric approach to trial design. Sensor‐based digital health technologies such as mobile spirometry (mSpirometry) are comparable to clinic spirometry for capturing outcomes, such as forced expiratory volume in 1 s (FEV1); however, the impact of remote spirometry measurements on the detection of treatment effect has not been investigated. A protocol for a multicenter, single‐arm, open‐label interventional trial of long‐acting beta agonist (LABA) therapy among 60 participants with uncontrolled moderate asthma is described. Participants will complete twice‐daily mSpirometry at home and clinic spirometry during weekly visits, alongside continuous use of a wrist‐worn wearable and regular completion of several diaries capturing asthma symptoms as well as participant‐ and site‐reported satisfaction and ease of use of mSpirometry. The co‐primary objectives of this study are (A) to quantify the treatment effect of LABA therapy among participants with moderate asthma, using both clinical spirometry (FEV1c) and mSpirometry (FEV1m); and (B) to investigate whether FEV1m is as accurate as FEV1c in detecting the treatment effect using a mixed‐effect model for repeated measures. Study results will help inform whether the deployment of mSpirometry and a wrist‐worn wearable for remote data collection are feasible in a multicenter setting among participants with moderate asthma, which may then be generalizable to other populations with respiratory disease.
How do maternal androgens and estrogens affect sex determination in reptiles with temperature‐dependent sex?
Authors: Valli Florencia E. , Simoncini Melina S. , González Marcela A. , Piña Carlos I. 
Published: August 2023

AbstractTemperature sex determination (TSD) in reptiles has been studied to elucidate the mechanisms by which temperature is transformed into a biological signal that determines the sex of the embryo. Temperature is thought to trigger signals that alter gene expression and hormone metabolism, which will determine the development of female or male gonads. In this review, we focus on collecting and discussing important and recent information on the role of maternal steroid hormones in sex determination in oviparous reptiles such as crocodiles, turtles, and lizards that possess TSD. In particular, we focus on maternal androgens and estrogens deposited in the egg yolk and their metabolites that could also influence the sex of offspring. Finally, we suggest guidelines for future research to help clarify the link between maternal steroid hormones and offspring sex.
Effects of NAD+ precursors on blood pressure, C‐reactive protein concentration and carotid intima‐media thickness: A meta‐analysis of randomized controlled trials
Authors: Lei Langhuan , Zhang Xiaoli , Lin Jiali , Liang Qiuyu , Sohouli Mohammad Hassan , Izze da Silva Magalhães Elma , Fatahi Somaye , Yang Lihua , Xu Wanting , Wang Xingyong , Li Wei , Yang Jianrong 
Published: August 2023

AbstractBackgroundThere are contradictory effects regarding the effect of NAD+ precursor on blood pressure and inflammation. In order to obtain a better viewpoint from them, this study aimed to comprehensively investigate the effects of NAD+ precursor supplementation on blood pressure, C‐reactive protein (CRP) and carotid intima‐media thickness (CIMT).MethodsPubMed/MEDLINE, Web of Science, SCOPUS and Embase databases were searched using standard keywords to identify all controlled trials investigating the effects of NAD+ precursor on blood pressure, CRP and CIMT. Pooled weighted mean difference (WMD) and 95% confidence intervals (95% CI) were achieved by random‐effects model analysis for the best estimation of outcomes.ResultsTwenty‐nine articles (with 8664 participants) were included in this article. Results from meta‐analyses of RCTs from random‐effects models indicated a significant reduction in systolic (SBP) (weighted mean difference (WMD): −2.54 mmHg, p < .001) and diastolic blood pressure (DBP) (WMD: −2.15 mmHg, p < .001), as well as in CRP (WMD: −.93 mg/L, 95% CI −1.47 to −.40, p < .001) concentrations and CIMT (WMD: −.01 mm, 95% CI −.02 to −.00, p = .005) with the NAD+ precursors supplementation compared with the control group. In addition, a greater effect of supplementation with NAD+ precursors in reducing blood pressure (BP) were observed with the highest dose (≥2 g) and duration of the intervention (>12 weeks), as well as with NA supplementation when compared to NE.ConclusionsOverall, these findings suggest that NAD+ precursor supplementation might have a beneficial effect on cardiovascular risk factors such as BP, CRP concentration and CIMT.
State‐owned enterprises and entrusted lending: Economic growth and business cycles in China
Authors: Zhang Shuonan 
Published: August 2023

AbstractA key economic structure in China is the co‐existence of state‐owned enterprises (SOEs) being bank‐favored firms as well as policy tools, and more productive private firms who can borrow from SOEs through entrusted lending. We explore macroeconomic implications of such a structure in China. Our findings suggest SOEs dampen output volatility at the cost of productivity volatility. In contrast, the healthy development of entrusted lending dampens variations of both output and productivity by reallocating credits between firms. Focusing on the recent growth slowdown in China, we further show conducive effects of entrusted lending on economic growth by mitigating capital misallocation.
Covid lockdowns and hospitalisations for oro‐facial trauma among children in Australia and the United Kingdom
Authors: Aminian Parmis , Kruger Estie , Tennant Marc 
Published: August 2023

AbstractBackground/AimThis retrospective study investigated the effect of lockdowns for Covid‐19 on the rates of oro‐facial trauma hospitalisations in children in Australia and the United Kingdom (UK).Materials and MethodsHospitalisation data for children up to 19 years old for oro‐facial trauma injuries (according to ICD‐10‐AM principal diagnosis codes) over 5 years were collected for Australian and UK children. The age‐standard rate (ASR) for each age group was calculated per 100,000 population.ResultsIn the younger Australian children, there was no statistically significant change in the ASR of oro‐facial trauma hospitalisations during the ‘Covid year’, compared to the average rate over the 3 years preceding. However, there was a significant decrease in the ASR of oro‐facial trauma hospitalisations during the ‘Covid year’ in older Australian children (aged 10–19), and for all UK children, except for infants under one‐year‐old.ConclusionsReduction of the ASR of hospitalisations due to oro‐facial trauma during the ‘Covid year’, and the implementation of lockdown measures in the two countries provides further evidence of the impact of physical activities and travel as contributing factors to oro‐facial injuries in children. In addition to its role in reducing the spread of virus infections during the pandemic, the lockdown measures also resulted in a simultaneous decrease in trauma injuries.
Thermal adaptation of microbial respiration persists throughout long‐term soil carbon decomposition
Authors: Li Jinquan , Pei Junmin , Fang Changming , Li Bo , Nie Ming 
Published: August 2023

AbstractSoil microbial respiration is expected to show adaptations to changing temperatures, greatly weakening the magnitude of feedback over time, as shown in labile carbon substrates. However, whether such thermal adaptation persists during long‐term soil carbon decomposition as carbon substrates decrease in decomposability remains unknown. Here, we conducted a 6‐year incubation experiment in natural and arable soils with distinct properties under three temperatures (10, 20 and 30°C). Mass‐specific microbial respiration was consistently lower under higher long‐term incubation temperatures, suggesting the occurrence and persistence of microbial thermal adaptation in long‐term soil carbon decomposition. Furthermore, changes in microbial community composition and function largely explained the persistence of microbial respiratory thermal adaptation. If such thermal adaptation generally occurs in large low‐decomposability carbon pools, warming‐induced soil carbon losses may be lower than previously predicted and thus may not contribute as much as expected to greenhouse warming.
Five‐year radiological findings from a randomized controlled trial of four periodontitis treatment strategies
Authors: Kubberød Jon Olav , Torgersen Gerald Ruiner , Gjermo Per , Baelum Vibeke , Preus Hans R. 
Published: August 2023

AbstractRadiographic findings from long‐term studies of periodontitis treatment have rarely been reported. Although bone destruction is a prominent feature of periodontitis, the long‐term effect on alveolar bone levels of different treatment strategies, with or without adjunctive metronidazole (MTZ), has not been reported. We investigated the 5‐year radiographic outcome of therapy in patient groups treated with conventional scaling and root planing (SRP) or same‐day full‐mouth disinfection (FDIS), with or without adjunctive MTZ. Following a 3‐month oral hygiene phase, 184 periodontitis patients were randomly allocated to one of four treatment regimens: (i) FDIS+MTZ; (ii) FDIS+placebo; (iii) SRP+MTZ; or (iv) SRP+placebo. Following active treatment, patients received biannual maintenance. In total, 161 patients (87.5%) completed the 5‐year follow‐up examination, at which the radiographic bone level (RBL), clinical attachment level, probing pocket depth, presence of plaque, and bleeding were recorded again. At the 5‐year follow up examination, minor radiological bone loss was observed in the intervention groups FDIS+placebo, SRP+MTZ, and SRP+placebo; by contrast, the FDIS+MTZ group did not show any change in RBL. Full‐mouth disinfection did not generally perform better than conventional SRP performed over a period of 2 to 4 weeks.
Chemical mutagenesis and thermal selection of coral photosymbionts induce adaptation to heat stress with trait trade‐offs
Authors: Scharfenstein Hugo J. , Alvarez‐Roa Carlos , Peplow Lesa M. , Buerger Patrick , Chan Wing Yan , van Oppen Madeleine J. H. 
Published: August 2023

AbstractDespite the relevance of heat‐evolved microalgal endosymbionts to coral reef restoration, to date, few Symbiodiniaceae strains have been thermally enhanced via experimental evolution. Here, we investigated whether the thermal tolerance of Symbiodiniaceae can be increased through chemical mutagenesis followed by thermal selection. Strains of Durusdinium trenchii, Fugacium kawagutii and Symbiodinium pilosum were exposed to ethyl methanesulfonate to induce random mutagenesis, and then underwent thermal selection at high temperature (31/33°C). After 4.6–5 years of experimental evolution, the in vitro thermal tolerance of these strains was assessed via reciprocal transplant experiments to ambient (27°C) and elevated (31/35°C) temperatures. Growth, photosynthetic efficiency, oxidative stress and nutrient use were measured to compare thermal tolerance between strains. Heat‐evolved D. trenchii, F. kawagutii and S. pilosum strains all exhibited increased photosynthetic efficiency under thermal stress. However, trade‐offs in growth rates were observed for the heat‐evolved D. trenchii lineage at both ambient and elevated temperatures. Reduced phosphate and nitrate uptake rates in F. kawagutii and S. pilosum heat‐evolved lineages, respectively, suggest alterations in nutrition resource usage and allocation processes may have occurred. Increased phosphate uptake rates of the heat‐evolved D. trenchii strain indicate that experimental evolution resulted in further trade‐offs in this species. These findings deepen our understanding of the physiological responses of Symbiodiniaceae cultures to thermal selection and their capacity to adapt to elevated temperatures. The new heat‐evolved Symbiodiniaceae developed here may be beneficial for coral reef restoration efforts if their enhanced thermal tolerance can be conferred in hospite.
The experiences of parents in an early‐intervention program for young people with borderline personality disorder features
Authors: Bosworth Chloe , Watsford Clare , Naylor Annaleise , Buckmaster Dean , Rickwood Debra 
Published: August 2023

AbstractPrevious research demonstrates that parents' communication skills may contribute to the development and maintenance of their young person's borderline personality disorder (BPD). Carers of people with BPD also experience their own psychosocial stressors and feel unsupported. Consequently, Dialectical Behavior Therapy for adolescents (DBT‐A) invites parents to partake in group therapy alongside their young person. Despite this involvement, little research exists examining parents' perspective of engaging in a DBT‐A program, and specifically whether they experience their own benefits and changes from being part of the program. To examine this, the current study interviews 34 parents who engaged in an early intervention DBT‐A program. Thematic analysis resulted in seven key themes and 16 subthemes beginning with parents' expectations of the program, followed by the key elements of the program that facilitated change, and the actual changes and benefits attributed to these elements. Overall, parents were surprised by their own gains from the program, and how the skills they learned facilitated personal development that improved family communication and functioning with their young person and more broadly. This study addresses the gap in understanding the parent perspective with clinical implications for the benefits of involving parents in therapy more generally.
Application of machine learning models for estimating the material parameters for multiaxial fatigue strength calculation
Authors: Nagode Marko , Papuga Jan , Oman Simon 
Published: August 2023

AbstractThis paper deals with a practical task of estimating missing material fatigue strengths required for the evaluation of multiaxial fatigue strength criteria, knowing other static or fatigue material parameters. Instead of searching for various analytical equations describing the dependencies between different material parameters, several machine learning models implemented in the caret R package are used here. The dataset used to train and test these models is based on the FatLim dataset with different material parameters, which has been redesigned for this new purpose. It is demonstrated that substantially more data points, such as were available in this study, are needed to achieve the goal set here. Although the results obtained at the current scale may be improved by the addition of new data points, the best performance of the random forest model rf and the worst performance of the pcr model are evident.
Indicators of academic readiness for college: Evaluating an advanced Spanish language program
Authors: Landes‐Lee Jill , Watzinger‐Tharp Johanna 
Published: August 2023

AbstractStudents who participate in advanced coursework in high school are more likely to enroll and persist in college. This study measured college readiness indicators of high school seniors enrolled in the Utah Spanish Bridge Program and analyzed whether performance differed based on race/ethnicity or on parent college completion. Findings show that race/ethnicity played a role in inequitable distribution of college readiness indicators to a larger extent than parent college completion. Hispanic students, although enrolled in advanced coursework at a higher rate than the school overall, were still at a disadvantage compared with their White peers. Findings can inform programs to improve educational outcomes and may suggest areas for future research.
Mitochondrial metabolism related markers GDF‐15, FGF‐21, and HIF‐1α are elevated in pediatric migraine attacks
Authors: Kilinc Yasemin Baranoglu , Kilinc Erkan , Danis Aysegul , Hanci Fatma , Turay Sevim , Ozge Aynur , Bolay Hayrunnisa 
Published: August 2023

AbstractObjectiveThe purpose of this study was to investigate the serum levels of mitochondrial metabolism/reactive oxygen species (ROS)–related peptides (hypoxia inducible factor‐1α [HIF‐1α], fibroblast growth factor‐21 [FGF‐21], growth differentiation factor‐15 [GDF‐15]) and key migraine‐related neuropeptides (calcitonin gene–related peptide [CGRP], pituitary adenylate cyclase‐activating peptide‐38 [PACAP‐38], substance P [SP], and vasoactive intestinal peptide [VIP]) during migraine attacks and to evaluate their diagnostic value in pediatric migraine.BackgroundThere is increasing evidence for the important role of impairment in oxidative mitochondrial metabolism in the pathophysiology of migraine. Potential biomarkers that may reflect the relationship between migraine and mitochondrial dysfunction are unclear.MethodsA total of 68 female pediatric migraine patients without aura and 20 female healthy controls aged 8–18 years, admitted to the hospital, were enrolled in this cross‐sectional study. Serum concentrations of these molecules were determined by enzyme‐linked immunosorbent assays, and clinical features and their possible diagnostic value were analyzed.ResultsSerum levels of HIF‐1α (252.4 ± 51.9 [mean ± standard deviation]) pg/mL), GDF‐15 (233.7 ± 24.7 pg/mL), FGF‐21 (96.1 ± 13.1 pg/mL), CGRP (44.5 ± 11.3), and PACAP‐38 (504.7 ± 128.9) were significantly higher in migraine patients compared to healthy controls (199.8 ± 26.8, 192.8 ± 20.7, 79.3 ± 4.1, 34.1 ± 3.5 and 361.2 ± 86.3 pg/mL, respectively). The serum levels of these peptides were also higher in patients with chronic migraine than in patients with episodic migraine, and higher in the ictal period than in the interictal period. A positive correlation was found between attack frequency and both HIF‐1α and FGF‐21 levels in migraine patients. Serum levels of VIP and SP were not different between the migraine patients and healthy controls.ConclusionMigraine attacks are accompanied by elevated HIF‐1α, FGF‐21, GDF‐15, CGRP, and PACAP‐38 in medication‐naive pediatric patients with migraine. Elevated circulating mitochondrial metabolism/ROS‐related peptides suggest a mitochondrial stress in pediatric migraine attacks and may have potential diagnostic value in monitoring disease progression and treatment response in children. Novel approaches intervening with mitochondrial metabolism need to be investigated.
Psychosocial aspects associated with self‐reported oral health in Brazilians older adults
Authors: Manica Laura Ramos , do Amaral Júnior Orlando Luiz , Fagundes Maria Laura Braccini , Menegazzo Gabriele Rissotto , do Amaral Giordani Jessye Melgarejo 
Published: August 2023

AbstractObjectivesOral health conditions can be determined by a series of factors that include psychosocial aspects. Therefore, this study aimed to investigate the association between psychosocial aspects and self‐reported oral health in Brazilian older adults.MethodsThe Brazilian Longitudinal Study of Ageing (ELSI‐Brazil) baseline data was analysed to develop this cross‐sectional study. The outcome was self‐reported oral health, and the psychosocial aspects included neighbourhood trust, life satisfaction and depressive symptoms. The associations were determined through Poisson regression models, controlling for socioeconomic, demographic and behavioural characteristics.ResultsA total of 9365 individuals with aged 50 years or over were included in this study. Individuals with good life satisfaction had 16% (PR: 0.84; 95% CI: 0.78–0.90) lower prevalence of having poor self‐reported oral health. Individuals who have depressive symptoms and do not trust in the neighbourhood presented 17% (PR: 1.17; 95% CI: 1.09–1.25) and 12% (PR: 1.12; 95% CI: 1.04–1.21) higher prevalence of poor self‐report oral health compared to those who did not show depressive symptoms and trust the neighbourhood.ConclusionOur findings suggest that positive psychosocial aspects are moderately associated with better self‐reported oral health. Therefore, we suggest that psychological health and social interaction should be present in public health policy actions.