bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disorder, Riot and Governance in Early Tudor London: Evil May Day, 1517
Authors: McSheffrey Shannon 
Published: August 2023

Abstract
On the eve of the May Day festival in 1517, a night of anti-immigrant violence broke out in London. Though pre-modern English historians have frequently invoked Evil May Day, as it came to be called, it has only recently been given concerted scholarly attention. The riot itself was ordinary, one amongst dozens in the first decades of the sixteenth century; it reveals, as a reflection of endemic grievance, a constellation of tensions surrounding labour, immigration, masculine identities, and governance in early Tudor London. More extraordinary, and heretofore not much noticed, was the Crown reaction to the Evil May Day disturbances, on which this article focuses. The insurrectionists were prosecuted for high treason rather than the minor charge of riot; a multi-day pageant of severe justice punctuated by theatrical reprieves followed. At least fifteen were put to death within London’s city walls and there are indications that the final execution toll was much greater, perhaps as high as forty-three. Such a toll following a mild riot could only have been unexpected and shocking, even if in fact ‘only’ fifteen rather than forty or more were executed. This was a message from the Crown both strategically conceived and emotionally driven by the king’s own rage, fury and fear. Though it was the youthful rioters who constituted most of those put to death in the aftermath, the message was meant primarily for the London civic elite, who had dared to question Crown policy and had failed to control the violence in their city.
Birth cohort changes in fertility ideals: evidence from repeated cross-sectional surveys in Finland
Authors: Golovina Kateryna , Nitsche Natalie , Berg Venla , Miettinen Anneli , Rotkirch Anna , Jokela Markus 
Published: August 2023

Abstract
Fertility has declined in developed countries but whether there is a similar pattern in the number of children individuals wish to have (henceforth an ideal number of children) remains unclear. Using repeated cross-sectional survey data from the Finnish Family Barometers, we examine birth cohort changes in the ideal number of children among men and women from five birth cohorts (1970–1974, 1975–1979, 1980–1984, 1985–1989, and 1990–1994). We also investigate whether associations between socioeconomic factors and the ideal number of children differ across birth cohorts, and whether there are any gender differences in these associations. We find that the ideal number of children is lower among more recent than earlier birth cohorts and that this shift is driven by substantially higher child-free ideals among recent cohorts. While the ideal number of children among men and women is associated with socioeconomic factors, birth cohort differences remain significant after controlling for these characteristics. These findings suggest that large birth cohort differences in child-free ideals are not due to the individual socioeconomic circumstances of more recent birth cohorts. Rather, it appears that the childbearing attitudes of Finns have changed and may have contributed to the recent fertility decline.
The association between daily step count and all-cause and cardiovascular mortality: a meta-analysis
Authors: Banach Maciej , Lewek Joanna , Surma Stanisław , Penson Peter E , Sahebkar Amirhossein , Martin Seth S , Bajraktari Gani , Henein Michael Y , Reiner Željko , Bielecka-Dąbrowa Agata , Bytyçi Ibadete 
Published: August 2023

Abstract

Aims
There is good evidence showing that inactivity and walking minimal steps/day increase the risk of cardiovascular (CV) disease and general ill-health. The optimal number of steps and their role in health is, however, still unclear. Therefore, in this meta-analysis, we aimed to evaluate the relationship between step count and all-cause mortality and CV mortality.

Methods and results
We systematically searched relevant electronic databases from inception until 12 June 2022. The main endpoints were all-cause mortality and CV mortality. An inverse-variance weighted random-effects model was used to calculate the number of steps/day and mortality. Seventeen cohort studies with a total of 226 889 participants (generally healthy or patients at CV risk) with a median follow-up 7.1 years were included in the meta-analysis. A 1000-step increment was associated with a 15% decreased risk of all-cause mortality [hazard ratio (HR) 0.85; 95% confidence interval (CI) 0.81–0.91; P &lt; 0.001], while a 500-step increment was associated with a 7% decrease in CV mortality (HR 0.93; 95% CI 0.91–0.95; P &lt; 0.001). Compared with the reference quartile with median steps/day 3867 (2500–6675), the Quartile 1 (Q1, median steps: 5537), Quartile 2 (Q2, median steps 7370), and Quartile 3 (Q3, median steps 11 529) were associated with lower risk for all-cause mortality (48, 55, and 67%, respectively; P &lt; 0.05, for all). Similarly, compared with the lowest quartile of steps/day used as reference [median steps 2337, interquartile range 1596–4000), higher quartiles of steps/day (Q1 = 3982, Q2 = 6661, and Q3 = 10 413) were linearly associated with a reduced risk of CV mortality (16, 49, and 77%; P &lt; 0.05, for all). Using a restricted cubic splines model, we observed a nonlinear dose–response association between step count and all-cause and CV mortality (Pnonlineraly &lt; 0.001, for both) with a progressively lower risk of mortality with an increased step count.

Conclusion
This meta-analysis demonstrates a significant inverse association between daily step count and all-cause mortality and CV mortality with more the better over the cut-off point of 3867 steps/day for all-cause mortality and only 2337 steps for CV mortality.
Stability of clustering of lifestyle risk factors in the Dutch adult population and the association with mental health
Authors: Dorsman Hannah , de Hollander Ellen , Wendel-Vos Wanda , van Rossum Caroline , Kemler Ellen , Hupkens Christianne , Hosper Karen , de Beurs Derek , Hiemstra Marieke 
Published: August 2023

Abstract

Background
Lifestyle factors often co-occur in clusters. This study examines whether clusters of lifestyle risk factors, such as smoking, alcohol use, physical inactivity, poor diet, sexual risk behaviour, cannabis and other drug use, change over time in a representative sample of Dutch adults. Additionally, the association between mental health and self-reported depression of lifestyle clusters was examined.

Methods
Each year cross-sectional data of approximately 7500 individuals of 18 years and older from the annual Dutch Health Survey of 2014–2019 were used. Clusters were determined by a two-step cluster analysis. Furthermore, regression analyses determined the association between clusters of lifestyle risk factors and mental health.

Results
Results show six clusters composed of one, multiple or no lifestyle risk factors. The clusters remained relatively stable over time: in some clusters, the number of people slightly changed between 2014 and 2019. More specifically, clusters that increased in size were the cluster with no lifestyle risk factors and the cluster with multiple lifestyle risk factors. Furthermore, results show that clusters with none to a few lifestyle risk factors were associated with better mental health and a lower prevalence of self-reported depression compared with clusters with multiple lifestyle risk factors.

Conclusions
The clustering of lifestyle risk factors remained stable over time. People with multiple lifestyle risk factors had poorer mental health than those without risk factors. These findings may emphasize the need for intervention strategies targeting this subgroup with multiple lifestyle risk factors.
Adaptation versus plastic responses to temperature, light, and nitrate availability in cultured snow algal strains
Authors: Broadwell Emily L M , Pickford Rachel E , Perkins Rupert G , Sgouridis Fotis , Williamson Christopher J 
Published: August 2023

Abstract
Snow algal blooms are widespread, dominating low temperature, high light, and oligotrophic melting snowpacks. Here, we assessed the photophysiological and cellular stoichiometric responses of snow algal genera Chloromonas spp. and Microglena spp. in their vegetative life stage isolated from the Arctic and Antarctic to gradients in temperature (5 – 15°C), nitrate availability (1 – 10 µmol L−1), and light (50 and 500 µmol photons m−2 s−1). When grown under gradients in temperature, measured snow algal strains displayed Fv/Fm values increased by ∼115% and electron transport rates decreased by ∼50% at 5°C compared to 10 and 15°C, demonstrating how low temperatures can mimic high light impacts to photophysiology. When using carrying capacity as opposed to growth rate as a metric for determining the temperature optima, these snow algal strains can be defined as psychrophilic, with carrying capacities ∼90% higher at 5°C than warmer temperatures. All strains approached Redfield C:N stoichiometry when cultured under nutrient replete conditions regardless of temperature (5.7 ± 0.4 across all strains), whereas significant increases in C:N were apparent when strains were cultured under nitrate concentrations that reflected in situ conditions (17.8 ± 5.9). Intra-specific responses in photophysiology were apparent under high light with Chloromonas spp. more capable of acclimating to higher light intensities. These findings suggest that in situ conditions are not optimal for the studied snow algal strains, but they are able to dynamically adjust both their photochemistry and stoichiometry to acclimate to these conditions.
Activity of root-knot nematodes associated with composition of a nematode-attached microbiome and the surrounding soil microbiota
Authors: Topalović Olivera , Bak Frederik , Santos Susana , Sikder Md Maniruzzaman , Sapkota Rumakanta , Ekelund Flemming , Nicolaisen Mette Haubjerg , Vestergård Mette 
Published: August 2023

Abstract
We investigated if activity of the pre-infective juveniles (J2s) of root-knot nematodes is linked to the recruitment of a specific microbiome on the nematode surface and/or to the composition of the surrounding microbiota. For this, we determined the J2 activity (active vs. non-motile, which referred to dead and immobile J2s) upon a 3-day incubation in soil suspensions and studied the composition of bacteria, protists, and fungi present on the nematode surface and in the suspensions using amplicon sequencing of the 16S/18S rRNA genes, and ITS region. We also amended suspensions with Pseudomonas protegens strain CHA0 to study its effects on J2 activity and microbial composition. The J2 activity was suppressed in soil suspensions, but increased when suspensions were amended with P. protegens CHA0. The active and non-motile J2s differed in the composition of surface-attached bacteria, which was altered by the presence of P. protegens CHA0 in the soil suspensions. The bacterial genera Algoriphagus, Pedobacter, and Bdellovibrio were enriched on active J2s and may have protected the J2s against antagonists. The incubation time appeared short for attachment of fungi and protists. Altogether, our study is a step forward in disentangling the complex nematode-microbe interactions in soil for more successful nematode control.
Magnitude and Mechanism of Phrenic Long-term Facilitation Shift Between Daily Rest Versus Active Phase
Authors: Marciante Alexandria B , Seven Yasin B , Kelly Mia N , Perim Raphael R , Mitchell Gordon S 
Published: August 2023

Abstract
Plasticity is a fundamental property of the neural system controlling breathing. One key example of respiratory motor plasticity is phrenic long-term facilitation (pLTF), a persistent increase in phrenic nerve activity elicited by acute intermittent hypoxia (AIH). pLTF can arise from distinct cell signaling cascades initiated by serotonin versus adenosine receptor activation, respectively, and interact via powerful cross-talk inhibition. Here, we demonstrate that the daily rest/active phase and the duration of hypoxic episodes within an AIH protocol have profound impact on the magnitude and mechanism of pLTF due to shifts in serotonin/adenosine balance. Using the historical “standard” AIH protocol (3, 5-min moderate hypoxic episodes), we demonstrate that pLTF magnitude is unaffected by exposure in the midactive versus midrest phase, yet the mechanism driving pLTF shifts from serotonin-dominant (midrest) to adenosine-dominant (midactive). This mechanistic “flip” results from combined influences of hypoxia-evoked adenosine release and daily fluctuations in basal spinal adenosine. Since AIH evokes less adenosine with shorter (15, 1-min) hypoxic episodes, midrest pLTF is amplified due to diminished adenosine constraint on serotonin-driven plasticity; in contrast, elevated background adenosine during the midactive phase suppresses serotonin-dominant pLTF. These findings demonstrate the importance of the serotonin/adenosine balance in regulating the amplitude and mechanism of AIH-induced pLTF. Since AIH is emerging as a promising therapeutic modality to restore respiratory and nonrespiratory movements in people with spinal cord injury or ALS, knowledge of how time-of-day and hypoxic episode duration impact the serotonin/adenosine balance and the magnitude and mechanism of pLTF has profound biological, experimental, and translational implications.
The Effect of the Human Brainstem Myelination on Gait Speed in Normative Aging
Authors: Akhonda Mohammad A B S , Faulkner Mary E , Gong Zhaoyuan , Laporte John P , Church Sarah , D’Agostino Jarod , Bergeron Jan , Bergeron Christopher M , Ferrucci Luigi , Bouhrara Mustapha 
Published: August 2023

Abstract
The brainstem functions as a relay and integrative brain center and plays an essential role in motor function. Whether brainstem tissue deterioration, including demyelination, affects motor function has not been studied. Understanding the potential relationship between brainstem demyelination and motor function may be useful for the early diagnosis of neurodegenerative diseases and to understand age-related gait impairments that have no apparent cause. In this work, we investigated the associations between rapid or usual gait speeds, as integrative measures of motor function, and cerebral myelin content. In 118 individuals (age 22–94 years) free of neurodegenerative diseases or cognitive impairment, myelin content was assessed as the myelin water fraction, a direct magnetic resonance imaging measure of myelin content, and longitudinal and transverse relaxation rates (R1 and R2), which are sensitive magnetic resonance imaging measures of myelin content. Our results indicate that participants with lower usual or rapid gait speed exhibited lower values of myelin water fraction and R1 in the main brainstem regions, which were more evident and statistically significant in the midbrain. In contrast, we found no significant associations between gait speeds and R2, an expected result because various physiological factors confound R2. These original findings provide evidence that the level of brainstem myelination may affect gait performance among cognitively unimpaired adults who are free from any clinically detectable neurodegenerative diseases. Further studies are needed to understand the longitudinal changes in brainstem myelination with aging and neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease.
Immigration, Racialization, and Asian American Older Adults’ Cognitive Difficulties
Authors: Nguyen Duy , Liu Rui , Lee Yookyong 
Published: August 2023

Abstract

Objectives
The sociohistorical experiences of Asian American older adults (AAOA) vary, but limited research has examined how immigration status operates as social determinants. This study builds on an existing framework to examine the relationship among social determinants and the differential effects of immigration status on cognitive difficulties among AAOA.

Methods
Using 5-year estimate data from the 2019 American Community Survey, the study sample consisted of AAOA aged 65 years and older identifying as Chinese, Filipino, Indian, Japanese, Korean, or Vietnamese (N = 100,584). AAOA were categorized as noncitizens, naturalized, or U.S. born for their immigration status. Participants who indicated having difficulty concentrating, remembering, or making decisions were categorized as having cognitive difficulties.

Results
Chinese were the most prevalent ethnic group (30%). Over 70% were naturalized citizens. About 8% endorsed having cognitive difficulties. According to bivariate analyses, there were considerable differences in AAOA’s characteristics (e.g., age, gender, marital status, medical insurance, employment status) by their immigration status. The results from the logistic regressions showed that immigration status was independently associated with poor cognitive difficulties. By AAOA’s immigration status, unique protective and risk factors were presented for cognitive difficulties.

Discussion
The differential pattern of cognitive difficulties among AAOA reveals a fuller picture of variations within AAOA by immigration status. The results imply that more research is needed to develop culturally sensitive practices that account for the racialized differences by AAOA’s immigration status. Further research into the interplay between structural determinants is necessary to formulate practice and policy interventions to address better successful aging for AAOA.
Polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) isozyme surface charge governs charge substrate preferences to modulate mucin type O-glycosylation
Authors: Ballard Collin J , Paserba Miya R , Paul Daniel Earnest James , Hurtado-Guerrero Ramón , Gerken Thomas A 
Published: August 2023

Abstract
A large family of polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) initiate mucin type O-glycosylation transferring α-GalNAc from a UDP-GalNAc donor to the hydroxyl groups of Ser and Thr residues of peptides and proteins, thereby defining sites of O-glycosylation. Mutations and differential expression of several GalNAc-Ts are associated with many disease states including cancers. The mechanisms by which these isozymes choose their targets and their roles in disease are not fully understood. We previously showed that the GalNAc-Ts possess common and unique specificities for acceptor type, peptide sequence and prior neighboring, and/or remote substrate GalNAc glycosylation. In the present study, the role of flanking charged residues was investigated using a library of charged peptide substrates containing the central -YAVTPGP- acceptor sequence. Eleven human and one bird GalNAc-T were initially characterized revealing a range of preferences for net positive, net negative, or unique combinations of flanking N- and/or C-terminal charge, correlating to each isozyme’s different electrostatic surface potential. It was further found that isoforms with high sequence identity (&gt;70%) within a subfamily can possess vastly different charge specificities. Enzyme kinetics, activities obtained at elevated ionic strength, and molecular dynamics simulations confirm that the GalNAc-Ts differently recognize substrate charge outside the common +/−3 residue binding site. These electrostatic interactions impact how charged peptide substrates bind/orient on the transferase surface, thus modulating their activities. In summary, we show the GalNAc-Ts utilize more extended surfaces than initially thought for binding substrates based on electrostatic, and likely other hydrophobic/hydrophilic interactions, furthering our understanding of how these transferases select their target.
Methodological reflections: developing the WHO database of violence against women policies
Authors: Watson Katherine , Burke Eva , Gold Judy , Eva Gillian , Amin Avni 
Published: August 2023

Abstract
This paper presents methodological reflections from the development of the World Health Organization (WHO) Violence against Women (VAW) Policies Database (hereinafter referred to as ‘the Database’) to inform future efforts to create similar public health policy databases for government accountability. Using the WHO Global Plan of Action on Violence accountability measures as a starting point, the Database was developed over a 2-year period in consultation with a reference group. A subset of indicators was piloted before finalization of a full list and the structure of the Database. Available VAW policies from 194 WHO Member States were reviewed by a team of consultants, who conducted content analysis and data entry. A ‘Manual and User Guide’ was developed to record decisions related to the processes for developing the Database. This guide was used to draw out key reflections in relation to policy indicators, inclusion criteria for policy documents, languages and analysis, quality assurance and sustainability. The process of developing the Database evolved iteratively in response to many factors, including the content of policies and the specificities of policy-making in each jurisdiction. Pragmatic decisions about the number of indicators and the types of policies to review were informed by pilot tests across a range of geographies. Standardization of analysis and data entry was ensured through the provision of in-depth guidance for researchers, and regular and open communication within the team was key to quality assurance. Online translation services enabled a review of policy documents in most languages. Documentation of the methodology ensured that others could replicate processes with fidelity in the future. Despite complexities, it is possible to develop a sound methodology for analysing the content of policy documents in a manner that yields findings that are useful in holding governments accountable for the commitments to address VAW and other public health issues in policy.
Characteristics of sows’ milk and piglet nutrient utilization during a 28-d lactation period
Authors: Chisoro Prince , Krogh Uffe , Theil Peter Kappel , Eskildsen Maria 
Published: August 2023

Abstract
The present study aimed to characterize the performance of suckling piglets from high prolific sows and investigate the impact of milk composition on piglet growth during a 4-wk lactation period. Piglet performance included weight gain (WG), milk intake, nutrients and energy in milk, and piglet energy metabolism in weeks 1 to 4 of lactation. Data from six previous experiments were used with a total of 2,047 piglets and 604 milk samples collected from 151 sows. Piglet body weight linearly increased (P &lt; 0.001) as lactation progressed, with the piglet WG being low in week 1 (132 g/d) and relatively constant from weeks 2 to 4 (248 g/d; P &lt; 0.001). The metabolizable energy (ME) intake of the piglets increased (P &lt; 0.001) from weeks 1 to 3; however, with lower values in week 4 than for week 3. The heat energy production and energy required for maintenance (MEm) linearly increased (P &lt; 0.001) from weeks 1 to 4. In addition, the retained energy and ME to MEm ratio only increased from weeks 1 to 2 (P &lt; 0.001) and then declined through week 4. Pearson correlation coefficients were used to examine the relations between the milk nutrient composition and the WG in weeks 1 to 4. The results showed that WG was negatively correlated with the milk protein concentration at all stages of lactation (P &lt; 0.001). In contrast, the WG was positively correlated with milk fat and lactose concentrations in weeks 2 and 4, respectively (P &lt; 0.001). In conclusion, the stage of lactation influenced WG, milk intake, nutrients in milk, energy in milk, and the energy metabolism of the suckling piglets. Moreover, maximizing milk protein concentration does not optimize piglet growth.