bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time-domain observation of interlayer exciton formation and thermalization in a MoSe2/WSe2 heterostructure
Authors: Conte Stefano Dal , Policht Veronica , Mittenzwey Henry , Dogadov Oleg , Katzer Manuel , Villa Andrea , Li Qiuyang , Kaiser Benjamin , Ross Aaron , Scotognella Francesco , Zhu Xiaoyang , Knorr Andreas , Selig Malte , Cerullo Giulio 
Published: April 2023

Abstract
Vertical heterostructures (HS) of transition metal dichalcogenides (TMDs) host interlayer excitons (ILX), with electrons and holes residing in different layers. With respect to their intralayer counterparts, ILX feature much longer lifetimes and diffusion lengths, paving the way to excitonic optoelectronic devices operating at room temperature. While the recombination dynamics of ILX has been intensively studied, the formation process and its underlying physical mechanisms are still largely unexplored. Here we use ultrafast transient absorption spectroscopy with a white-light probe, spanning both intralayer and interlayer exciton resonances, to simultaneously capture and time-resolve interlayer charge transfer and ILX formation dynamics in a MoSe2/WSe2 HS. We find that the ILX formation timescale is nearly an order of magnitude (∼1 ps) longer than the interlayer charge transfer time (∼100 fs). Microscopic calculations attribute the relative delay to an interplay between phonon-assisted interlayer exciton cascade and subsequent cooling processes, and excitonic wave-function overlap. Our results provide an explanation to the efficient photocurrent generation observed in optoelectronic devices based on TMD HS, as the ILX have an opportunity to dissociate during their thermalization process.
Identification of Key Extracellular Proteins and miRNAs for Use as Diagnostic Biomarkers of Temporomandibular Joint Osteoarthritis
Authors: Wang Zhihan , Tuerxun Palati , Jia Xiaoshi , zhao ke 
Published: April 2023

Abstract
Background: Temporomandibular joint osteoarthritis (TMJ-OA) is an inflammatory disease that is pathologically characterized by the destruction of cartilage and subchondral bone. During the progression of TMJ-OA, the concentration of some extracellular proteins (EPs) in the synovial fluid are altered in the inflammatory environment. Micro RNAs formed an important epigenetic regulation mode during disease progression, which were secreted by cells by means of vesicles then regulated gene transcription. However, the relationship among these proteins, micro RNAs and inflammation was still unclear.
Methods: In this study, bioinformatics tools were used to compare the expression profiles of synovial membranes from human TMJ-OA samples and normal TMJ samples.
Results: Four genes, including MMP2, FN1, TIMP1, and APOE were identified as hub EPGs. Four miRNAs were identified as key differentially expressed miRNAs (kDEMs) and formed a miRNA-mRNA network with four hEPGs. Furthermore, after stimulation of chondrocytes and SW982 cells with IL-1β in vitro, the gene and protein expression levels of the hEPGs were increased and the four kDEMs were decreased compared with those in the normal group.
Conclusion: The study of differentially expressed EPs and miRNAs in TMJOA is of great significance for clinical diagnosis and treatment.
No increase in translocated chromosomal aberrations, an indicator of ionizing radiation exposure, in childhood thyroid cancer in Fukushima Prefecture
Authors: Sakai Akira , Tsuyama Naohiro , Ohira Tetsuya , Sugai-Takahashi Misaki , Ohba Takashi , Azami Yusuke , Matsumoto Yoshiko , Iwadate Manabu , Suzuki Satoshi , Sato Maki , Hosoya Mitsuaki , Ishikawa Tetsuo , Suzuki Shinichi 
Published: April 2023

Abstract
To investigate the effects of radiation exposure due to the Fukushima nuclear power plant accident, following the disaster Fukushima Prefecture launched thyroid ultrasound examinations of residents who were generally younger than 18 years at the time of the earthquake. As the rate of pediatric thyroid cancer was higher than expected, we conducted biological dose assessment based on the frequency of translocated chromosome (Tr) aberrations using peripheral blood lymphocytes. Tr formation frequency was compared among the thyroid cancer (n = 38, median age 18 years, age range 12–26 years), thyroid-related disease (n = 30, median age 21 years, age range 15–28 years), and healthy controls (n = 32, median age 21 years, age range 20–23 years) groups. Tr aberration frequency was initially significantly higher in the thyroid cancer than in the other two groups; however, differences among the groups disappeared after adjusting for history of CT scan, as 92%, 67%, and 28% of those in the thyroid cancer, thyroid-related disease, and control groups, respectively, had undergone CT previously. Therefore, the significant difference in the initial number of Tr formations is presumably due to radiation exposure from CT. Accordingly, the effects of medical exposure on the chromosomes of children and adolescents should be noted.
Molecular networking-based drug repurposing strategies for SARS-CoV-2 infection by targeting alpha-1-antitrypsin (SERPINA1)
Authors: Parameswaran Dakshinesh , Gurumoorthy Premkumar Kuduva , Munusamy Ravikumar , Thangavelu Saravanan , Selvaraj Jubie , Akey Krishna Swaroop , Chellappa Selvinthanuja , Vivekanandan Lalitha , Thangavelu Prabha 
Published: April 2023

Abstract
Background For a deeper comprehension of the condition and the development of more potent therapies, it is essential to understand COVID-19 pathogenesis. Transmembrane serine protease 2 (TMPRSS2) and disintegrin and metalloproteinase 17 (ADAM17) are two of the most significant proteases in the pathogenesis of COVID-19. An intrinsic tissue protector with antiviral and anti-inflammatory effects is called alpha-1-antitrypsin (A1AT), and it inhibits the protein TMPRSS2, which is crucial for SARS-CoV-2-S protein priming and viral infection. It also prevents the activity of pro-inflammatory chemicals like neutrophil elastase, TNF-, and IL-8.Objective According to current findings, repurposing available medications will result in more effective functioning than using newly designed medications. Based on this, we used FDA-approved drugs and did a computational study to find out what role A1AT plays in SARS-CoV-2 infections and how it stops Covid-19 from spreading.Method This computational study comprises the screening of FDA approved drugs by using molecular networking studies via cytoscape version 3.9.1 to identify any drugs binding interactions with SERPINA1, a gene that provides instructions for making a protein called A1AT, which is a type of serine protease inhibitor, followed by the generation of a pharmacophore model, virtual screening, and docking studies.Result The 22 compounds that were selected from this molecular-networking model were subjected to pharmacophore modelling followed by virtual screening. Through this screening, we have selected 22 molecules based on the Lipinski rule and low RMSD value, i.e., below 0.069235 Ao. From the ZINC database, the top six molecules discovered were found to have a higher affinity for A1AT when compared to the co-crystal ligand (-12.8236). The highest scores obtained by alpha-1-antitrypsin (PDB ID: 7NPK) are − 22.0254 and − 21.676 for ZINC00896543 and ZINC05316843, respectively.Conclusion Consequently, the molecules found by using different software programmes may be employed to control and treat COVID 19. By increasing the levels of A1AT, we may thus infer that these molecules have excellent action in the reversal of COVID-19.
The ViaHole Technique: A Novel Approach for Recanalizing Major Side Branch Occluded by Viabahn Stent-Graft
Authors: Haraguchi Takuya , Tsujimoto Masanaga , Otake Ryo , Kashima Yoshifumi , Sato Katsuhiko , Fujita Tsutomu 
Published: April 2023

Abstract
Background: Heparin-bonded endoluminal stent-graft covered with expanded polytetrafluoroethylene (ePTFE) has been utilized to manage arterial rupture. Stent-graft implantation might require crossing a major branch for hemostasis, potentially causing limb ischemia. We propose the ViaHole technique as a novel recanalization method for major branch occlusion.
Main text: A 72-year-old male with a history of right popliteal aneurysm treatment involving stent-graft implantation suffered from acute limb ischemia (ALI). Imaging revealed occlusion from the distal superficial femoral artery (SFA) to below-the-knee arteries. During thrombus removal attempts by surgical thrombectomy, an unintentional rupture occurred in the proximal posterior tibial artery (PTA). Surgical bypass was infeasible due to no run-off in below-the-knee arteries. Hemostasis was succeeded by endoluminal stent-grafts implantation from the tibioperoneal trunk to the proximal PTA. However, recurrent ALI occurred three months later. Surgical bypass was again deemed unfeasible due to no distal anastomosis site. After unsuccessful recanalization attempts for bilateral tibial arteries, the decision was made to recanalize the peroneal artery occlusion using the ViaHole technique.
This technique involved several steps: 1) advancing retrograde devices into the peroneal artery occlusion, 2) guiding the retrograde guidewire to touch the outer surface of the posterior wall of the ePTFE on the stent-graft at the peroneal arterial ostium, 3) puncturing the stent-graft completely using a 20-gauge needle to touch the retrograde device, 4) manipulating the retrograde guidewire through the needle hole and externalizing it, 5) advancing the retrograde microcatheter over the guidewire and through the hole of the ePTFE into the tibioperoneal trunk, 6) catching the retrograde microcatheter using an antegrade 4-Fr catheter, 7) inserting an antegrade guidewire into the retrograde microcatheter, 8) advancing an antegrade microcatheter into the peroneal artery, 9) dilating the lesion and stent-graft hole using a 3.0-mm noncompliant balloon, 10) ensuring hemostasis by inflating the 3.0-mm balloon to achieve hemostasis at the puncture site. The final angiogram demonstrated sufficient blood flow, resulting in symptom improvement. The patient was discharged on the third postoperative day, and 1-year patency was confirmed.
Conclusions: The ViaHole technique may be valuable for revascularizing a major side branch occluded by stent-graft implantation.
ACSL1 Regulation of Ferroptosis in Clear Cell Renal Cell Carcinoma via the HO-1/GPX4 Axis Mechanism
Authors: Wang Zicheng , Zhao Leizuo , Kang Weiting , Xu Yingkun , Zhang Peizhi , Deng Wentao , Peng Fan , Hao Yuhu , Xia Qinghua 
Published: April 2023

Abstract
Objective This study aimed to evaluate the expression level of long chain acyl-CoA synthase 1 (ACSL1) in clear cell renal cell carcinoma (ccRCC) tissue and explore its biological role in the progression of ccRCC.Methods Using Reversed Phase Protein Array (RPPA) sequencing technology, we identified ACSL1 as the target gene of interest. We then used the TCGA database to analyze the mRNA expression level of ACSL1 in ccRCC tissue and its clinical relevance. Immunohistochemistry and qRT-PCR were used to measure the expression level of ACSL1 in ccRCC tissue and investigate the correlation between ACSL1 expression level and clinicopathological characteristics and patient prognosis. CCK-8 technology and ferrostatin-1 were used to investigate the correlation between ACSL1 and ferroptosis in renal cancer cells. We also measured the content of malondialdehyde, glutathione, reactive oxygen species level, and degree of mitochondrial damage under electron microscopy to detect the effect of ACSL1 on ferroptosis of renal cancer cells. Additionally, we used RNA-Seq and Western blotting techniques to explore the potential mechanism of ACSL1 in renal cancer cells. Finally, we investigated the effect of ACSL1 on tumor growth using a xenotransplantation model.Results Our results showed that the expression level of ACSL1 in ccRCC tissue was significantly decreased and was correlated with clinical characteristics. The low expression level of ACSL1 was associated with poor patient prognosis. Overexpression of ACSL1 in renal cancer cells led to a significant decrease in GSH content, an improvement in the ability of lipid peroxidation, a significant increase in ROS level, significant shrinkage of intracellular mitochondria, and decreased expression of GPX4 and SLC7A11. RNA-Seq and KEGG enrichment analysis revealed that ACSL1 regulates ferroptosis in ccRCC through the HO-1/GPX4 axis. Western blotting confirmed that ACSL1 upregulated the expression of HO-1 and inhibited the expression of GPX4.Conclusion The expression of ACSL1 is low in human ccRCC tissue, and ACSL1 may be a potential target and prognostic marker for the treatment of ccRCC.
Role of ER-Mitochondria interface in the regulation of Glioma Stem Cells
Authors: Turos-Cabal Maria , Sanchez-Sanchez Ana Maria , Puente-Moncada Noelia , Herrera Federico , Rodriguez-Blanco Jezabel , Antolin Isaac , Alvarez-Vega Marco Antonio , Rodriguez Carmen , Martín Vanesa 
Published: April 2023

Abstract
Purpose: Glioblastoma (GBM) treatment is extremely challenging due to the high complexity of the tumor, being one of the tumors in which a subpopulation of highly resistant cancer initiating cells (GICs) has been clearly identified. Thus, understanding the differences between GICs and tumor bulk cells is therefore essential to move to less conventional but more efficient approaches.
Methods: fluorimetry was used to measure glucose uptake, mitochondrial calcium, intracellular oxidants and mitochondrial membrane potential. Spectrophotometry was used to monitor lactate dehydrogenase activity. Self-renewal was determined by the limiting dilution assay and cell death by trypan blue exclusion assays. Protein expression was determined by western blot while gene expression was determined by real-time PCR.
Results: We found that, unlike their differentiated progeny, GICs survival and stemness depend on mitochondrial metabolism. GICs present higher glucose uptake and mitochondrial membrane potential and less LDH activity, being more sensitive to mitochondrial inhibition than their differentiated counterparts. Calcium flux seems to play an essential role in the maintenance of this distinct metabolic phenotype with a decrease in the expression of VDAC and Grp75, two of the main proteins in the transfer of calcium from endoplasmic reticulum (ER) to the mitochondria. Disruption of ER homeostasis using ER stress inducers or inhibition of ER-mitochondrial contact sites using the Grp75 inhibitor MKT-707 resulted in GICs cytotoxicity and loss of stemness. Moreover, MKT-077 also potentiates the effect of temozolomide, current treatment for glioblastoma.
Conclusions: our data indicates that ER-mitochondrial homeostasis is essential for regulation of GICs metabolism, survival and stemness.
Post retention strength of apical and conventional coating obturation method using bioceramic sealer: A laboratory investigation.
Authors: Chanapairin Benjarat , Kulvitit Sirinya , Sathorn Chankhrit 
Published: April 2023

Abstract
Objectives
Bioceramic sealer (BCS) occupied dentinal tubules have been shown to reduce fibre post retention strength. Coating gutta percha with BCS only on apical portion leaving middle and coronal part of the canal unsmeared by BCS may improve post retention strength due to increased retention strength between dentin and resin cement interface. The purpose of the study was to test if this was the case.
Materials and Methods
Twenty seven extracted human mandibular premolars were instrumented and randomly assigned to three obturation methods: conventional coating (CC), non coating (NC), and apical coating (AC). After post cementation, two samples were prepared at apical, middle, and coronal root level (one for scanning electron microscope (SEM) study and another push out bond strength (PBS) test). After PBS test, failure modes were determined by stereo microscope: dentine-cement (DC), post-cement (PC) and mixed. PBS data were analysed by One way ANOVA for the specific effects of obturation methods. Repeated ANOVA was used for the specific effects of root levels on PBS in different obturation methods.
Results
PBS of CC at the middle 1/3 was significantly higher than the apical 1/3. PBS of AC and NC was significantly higher than CC group at apical 1/3 and coronal 1/3 level. DC was found most in CC group while PC was found most in NC and AC group.
Conclusions and Clinical Relevance
Apical coating obturation method under operating microscope had significantly higher PBS than conventional coating method. This may reduce the chance of fibre post dislodgement.
PD-1 Inhibitor Induced Hypertrophic Lichen Planus: A Case Study
Authors: Lim Olivia , Maher Eamonn , Miller Daniel D. 
Published: April 2023

Abstract
PD-1 inhibitors have revolutionized the treatment of cancer and are being employed to treat an expanding array of cancers. Therefore, to best serve our patients, we must familiarize ourselves with the spectrum of skin manifestations associated with PD-1 inhibitor therapy. We report a unique case of hypertrophic lichen planus in a 64-year-old male treated with pembrolizumab that initially presented with a keratoacanthoma (KA) morphology before evolving into a more typical hypertrophic lichen planus. The patient had symptoms of mucositis for 4 months until nodules and plaques began emerging on his shins and dorsal hands, at which time the patient was referred to dermatology. Initial biopsy of shin nodule looked histologically compatible to a keratoacanthoma with lichenoid inflammation. Mohs surgery was performed on the patient's hands due to concerns of functional impairment. However, the lesions within the surgical site recurred rapidly. Repeat biopsy was performed of both the hand and the shin. Histopathology revealed features consistent with hypertrophic lichen planus. By this time, lower extremity lesions that initially appeared as KA-like had progressed to a more typical appearing hypertrophic lichen planus. Therapy was started with topical clobetasol and acitretin. All lesions resolved within 1–2 months. This case highlights the importance of maintaining a high suspicion for lichenoid reactions as sequelae to PD-1 inhibitors and displays how these reactions do not always present in their classical forms initially, and can transform over time.
Spray-coated Inorganic Lead-free Double Perovskite Cs2AgBiBr6 Based Large-scale Triboelectric Nanogenerator for Enhanced Energy Harvesting
Authors: Wang Mengrou , Zhao Jingda , Xu Yubing , Wang Xin , Onwudiwe Damian Chinedu , Fayemi Omolola Esther , Elemike Elias Emeka , Bae Byung Seong , Zhu Ying , Zhu Zhuoya , Zhao Zhiwei , Li Qing , Lei Wei 
Published: April 2023

AbstractLow-cost, handily prepared, and efficient large-scale triboelectric nanogenerator (TENG) is considered as the new scheme for distributed mechanical conversion or renewable blue energy utilization. Semiconductors with high carrier mobility introduction potency overcome pure polymer restriction for uncompetitive short current density. An extremely popular all-inorganic lead-free double perovskite Cs2AgBiBr6(CABB) has emerged as extraordinary potential material in the substitution of semiconductor triboelectric material, which overcomes the limitations of high impedance associated with organic polymer insulator-based materials. In this current study, assembled by CABB which was certified as an available positive frictional material, TENG with a sandwiched structure of ITO/c-TiO2(compact TiO2)/m-TiO2(mesoporous TiO2)/CABB - the poly tetra fluoroethylene (PTFE)/Al exhibits appropriate performance on environmental stability and output capacity. A comparison of the fabrication process showed that spraying is an inexpensive method to prepare large-scale functional films of CABB TENG with brilliant relative dielectric constant and work function (Wf) difference that possess more distinguished output characteristics. This was confirmed by the appearance of higher open-circuit voltage of 105 V, larger short-current density of 2.45 mA/m2at 0.25 Hz motion parameter, and more abundant power density output of 0.76 W/m2under a higher frequency of 10 Hz. Further study clearly confirmed that both higher frequency and larger contact area are conducive to the total output power, while terminal charging speed is inversely or positively proportional with capacitance or mechanical frequency. The final physical display effect showed that spraying with CABB TENG could light up at least 53 commercial yellow LEDs, holding decent energy conversion ability. This confirms its efficiency, high throughput, and cost efficiency.
Diagnostic value of new combination of methylated Septin9 with LMR in adenomatous polyps and stage I to IV colorectal cancer
Authors: Qu Qingyun , Sun Qian 
Published: April 2023

Abstract
Purpose To explore the diagnostic value of tumor markers, inflammatory indicators and methylated Septin9 gene (mSEPT9) alone and combination in adenomatous polyps and stage I to IV colorectal cancer (CRC).
Methods Data of mSEPT9, blood routine inflammatory indicators (NLR, LMR, MPV/PC), tumor markers (CEA, CA125, CA19-9) and pathological stage from 420 patients with CRC, 61 patients with adenomatous polyps and 175 healthy people from 2018 to 2022 were retrospectively analyzed. Receiver operating characteristic (ROC) curve was plotted for the assessment of diagnostic accuracy, and statistical data were processed by SPSS 26.0 software.
Results The levels of NLR, CEA and CA19-9 in CRC group were significantly higher than those in healthy group, with statistical significance (P < 0.05). The levels of LMR and mSEPT9 in healthy group, polyps group and CRC group were decreased gradually, with statistical among all groups (P < 0.05). With the progressive of CRC from stage I to IV, the LMR levels and Ct value of mSEPT9 decreased gradually, and mSEPT9 in stage I/II, III and IV groups was significantly different (P < 0.05). The sensitivity of mSEPT9 increased gradually with the progression of stage, from 56.1% in stage I to 100% in stage IV. MSEPT9 was the most valuable single indicator in the diagnosis of CRC, and its sensitivity, specificity and AUC were 70.0%, 94.3% and 0.830, respectively. LMR was the most valuable single indicator in the diagnosis of adenomatous polyps, and its sensitivity, specificity and AUC were 78.7%, 57.1% and 0.732, respectively. MSEPT9 combined with LMR was the most valuable combination in the diagnosis of CRC and adenomatous polyps, and its sensitivity, specificity and AUC were 82.3% and 82.0%, 90.3% and 54.9%, 0.917 and 0.759, respectively.
Conclusions The new combination of mSEPT9 with LMR showed the best diagnostic value in the whole process of CRC occurrence and development, it should be used as the first choice for high-risk population screening, adjuvant diagnosis of CRC and disease staging.