bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cultured CD71+ erythroid cells modulate the host immune response
Authors: Alshalani Abdulrahman , Beuger Boukje M. , van Bruggen Robin , Acker Jason P. , Juffermans Nicole P. 
Published: March 2023

AbstractObjectiveThe study aimed to determine the impact of Red Blood Cells (RBCs) generated from peripheral blood mononuclear cells (PBMCs) on T cell proliferation and host response following whole blood stimulation.BackgroundCulturing RBCs is a potential solution for donor shortage. The impact of immature cultured RBCs which express CD71+ on host immune response is not known.Methods/MaterialsPBMCs were seeded in an erythroid expansion medium. CD71+ cells were isolated at days 14 and 21 of culture and incubated with either purified T cells or with LPS‐stimulated whole blood. Controls were incubated with medium.ResultsAt day 9, the percentage of cells that expressed CD45 and CD71 reached to the highest level (32.9%, IQR; 26.2–39.05) while the percentage of cells that expressed CD71 and CD235a reached to the highest level on day 17 (70.2%, IQR; 66.1–72.8). Incubation of T cells with days 14 CD71+ cells and day 21 CD71+ cells increased T cell proliferation. In a whole blood stimulation assay, day 21 CD71+ cells, but not day 14 CD71+ cells, inhibited the production of IL‐6 and TNFα.ConclusionCultured erythroid cells can modulate the immune response by promoting T cell proliferation and inhibiting cytokine secretions following whole blood stimulation.
OsNF‐YA3 regulates plant growth and osmotic stress tolerance by interacting with SLR1 and SAPK9 in rice
Authors: Jin Xinkai , Zhang Yifan , Li Xingxing , Huang Junli 
Published: March 2023

SUMMARYThe antagonism between gibberellin (GA) and abscisic acid (ABA) signaling pathways is vital to balance plant growth and stress response. Nevertheless, the mechanism by which plants determine the balance remains to be elucidated. Here, we report that rice NUCLEAR FACTOR‐Y A3 (OsNF‐YA3) modulates GA‐ and ABA‐mediated balance between plant growth and osmotic stress tolerance. OsNF‐YA3 loss‐of‐function mutants exhibit stunted growth, compromised GA biosynthetic gene expression, and decreased GA levels, while its overexpression lines have promoted growth and enhanced GA content. Chromatin immunoprecipitation–quantitative polymerase chain reaction analysis and transient transcriptional regulation assays demonstrate that OsNF‐YA3 activates GA biosynthetic gene OsGA20ox1 expression. Furthermore, the DELLA protein SLENDER RICE1 (SLR1) physically interacts with OsNF‐YA3 and thus inhibits its transcriptional activity. On the other side, OsNF‐YA3 negatively regulates plant osmotic stress tolerance by repressing ABA response. OsNF‐YA3 reduces ABA levels by transcriptionally regulating ABA catabolic genes OsABA8ox1 and OsABA8ox3 by binding to their promoters. Furthermore, OSMOTIC STRESS/ABA‐ACTIVATED PROTEIN KINASE 9 (SAPK9), the positive component in ABA signaling, interacts with OsNF‐YA3 and mediates OsNF‐YA3 phosphorylation, resulting in its degradation in plants. Collectively, our findings establish OsNF‐YA3 as an important transcription factor that positively modulates GA‐regulated plant growth and negatively controls ABA‐mediated water‐deficit and salt tolerance. These findings shed light on the molecular mechanism underlying the balance between the growth and stress response of the plant.
Systemic development of wheat–Thinopyrum elongatum translocation lines and their deployment in wheat breeding for Fusarium head blight resistance
Authors: Guo Xianrui , Shi Qinghua , Liu Yang , Su Handong , Zhang Jing , Wang Mian , Wang Chunhui , Wang Jing , Zhang Kaibiao , Fu Shulan , Hu Xiaojun , Jing Donglin , Wang Zhen , Li Jinbang , Zhang Pingzhi , Liu Cheng , Han Fangpu 
Published: March 2023

SUMMARYFusarium head blight (FHB), mainly caused by Fusarium graminearum, is one of the most destructive diseases of wheat (Triticum aestivum) around the world. FHB causes significant yield losses and reduces grain quality. The lack of resistance resources is a major bottleneck for wheat FHB resistance breeding. As a wheat relative, Thinopyrum elongatum contains many genes that can be used for wheat improvement. Although the novel gene Fhb‐7EL was mapped on chromosome 7EL of Th. elongatum, successful transfer of the FHB resistance gene into commercial wheat varieties has not been reported. In this study, we developed 836 wheat–Th. elongatum translocation lines of various types by irradiating the pollen of the wheat–Th. elongatum addition line CS‐7EL at the flowering stage, among which 81 were identified as resistant to FHB. By backcrossing the FHB‐resistant lines with the main cultivar Jimai 22, three wheat–Th. elongatum translocation lines, Zhongke 1878, Zhongke 166, and Zhongke 545, were successfully applied in wheat breeding without yield penalty. Combining karyotype and phenotype analyses, we mapped the Fhb‐7EL gene to the distal end of chromosome 7EL. Five molecular markers linked with the FHB resistance interval were developed, which facilitates molecular marker‐assisted breeding. Altogether, we successfully applied alien chromatin with FHB resistance from Th. elongatum in wheat breeding without yield penalty. These newly developed FHB‐resistant wheat–Th. elongatum translocation lines, Zhongke 1878, Zhongke 166, and Zhongke 545, can be used as novel resistance resources for wheat breeding.
The effectiveness of patient activation interventions in adults with chronic kidney disease: A systematic review and meta‐analysis
Authors: Lunardi Laura E. , Hill Kathy , Xu Qunyan , Le Leu Richard , Bennett Paul N. 
Published: March 2023

AbstractBackgroundChronic kidney disease (CKD) is a complex health condition that profoundly impacts an individual's general health and well‐being throughout their entire lifetime. People with CKD require the knowledge, confidence, and skills to actively self‐manage their health. This is referred to as patient activation. The efficacy of interventions to increase patient activation in the CKD population is unclear.AimThis study aimed to examine the effectiveness of patient activation interventions on behavioral health‐related outcomes among people with CKD stages 3–5.MethodsA systematic review and meta‐analysis of randomized controlled trials (RCTs) of patients with CKD stages 3–5 was performed. MEDLINE, EMCARE, EMBASE, and PsychINFO databases were searched between 2005 and February 2021. Risk of bias was assessed using the Joanna Bridge Institute critical appraisal tool.ResultsNineteen RCTs that enrolled 4414 participants were included for synthesis. Only one RCT reported patient activation using the validated 13‐item patient activation measure (PAM–13). Four studies demonstrated strong evidence that the intervention group developed a higher level of self‐management compared to the control group (standardized mean differences [SMD] = 1.12, 95% CI [0.36, 1.87], p = .004). Eight RCTs led to a significant improvement in self‐efficacy (SMD = 0.73, 95% CI [0.39, 1.06], p < .0001). There was weak to no evidence on the effect of the strategies shown on the physical component and mental components of health‐related quality of life, and medication adherence.Linking Evidence to ActionThis meta‐analysis highlights the importance of including tailored interventions using a cluster approach including patient education, goal setting with individualized action plan, and problem‐solving to engage patients to be more actively involved in the self‐management of their CKD.
Bubble wall confinement–driven molecular assembly toward sub–12 nm and beyond precision patterning
Authors: Qu Zhiyuan , Zhou Peng , Min Fanyi , Chen Shengnan , Guo Mengmeng , Huang Zhandong , Ji Shiyang , Yan Yongli , Yin Xiaodong , Jiang Hanqiu , Ke Yubin , Zhao Yong Sheng , Yan Xuehai , Qiao Yali , Song Yanlin 
Published: March 2023

Patterning is attractive for nanofabrication, electron devices, and bioengineering. However, achieving the molecular-scale patterns to meet the demands of these fields is challenging. Here, we propose a bubble-template molecular printing concept by introducing the ultrathin liquid film of bubble walls to confine the self-assembly of molecules and achieve ultrahigh-precision assembly up to 12 nanometers corresponding to the critical point toward the Newton black film limit. The disjoining pressure describing the intermolecular interaction could predict the highest precision effectively. The symmetric molecules exhibit better reconfiguration capacity and smaller preaggregates than the asymmetric ones, which are helpful in stabilizing the drainage of foam films and construct high-precision patterns. Our results confirm the robustness of the bubble template to prepare molecular-scale patterns, verify the criticality of molecular symmetry to obtain the ultimate precision, and predict the application potential of high-precision organic patterns in hierarchical self-assembly and high-sensitivity sensors.
Supplemental Material: Shallow and deep subsurface sediment remobilization and intrusion in the Middle Jurassic to Lower Cretaceous Agardhfjellet Formation (Svalbard)
Authors: Ogata K. , al. et 
Published: March 2023

<p>File S1: Sample locations. File S2A and File S2B: Petrographic, mineralogical, and micromorphological data. File S3: Digital outcrop models. </p>
What are the characteristics of vitamin D metabolism in opioid dependence? An exploratory longitudinal study in Australian primary care
Authors: Reece Albert Stuart , Hulse Gary Kenneth 
Published: March 2023

ObjectiveCompare vitamin D levels in opioid dependence and control population and adjust for relevant confounding effects. Nuclear hormone receptors (including the vitamin D receptor) have been shown to be key transducers and regulators of intracellular metabolism and comprise an important site of pathophysiological immune and metabolic dysregulation potentially contributing towards pro-ageing changes observed in opioid-dependent patients (ODPs).DesignLongitudinal prospective comparing ODPs with general medical controls (GMCs).SettingPrimary care.ParticipantsProspective review comparing 1168 ODP (72.5% men) and 415 GMC (51.6% men, p<0.0001). Mean ages were 33.92±0.31 (mean±SEM) and 41.22±1.32 years, respectively (p<0.0001). Opioid use in the ODP has been previously reported and shown to be typical.InterventionsNil. Observational study only.Primary and secondary outcomesSerum vitamin D levels and relevant biochemical parameters.ResultsVitamin D levels were higher in the ODP (70.35±1.16 and 57.06±1.81 nmol/L, p<0.0001). The difference in ages between the two groups was handled in an age-matched case–control subanalysis and also by multiple regression. Sexes were analysed separately. The age:status (or age:time:status) was significant in case–control, cross-sectional and longitudinal analyses in both sexes (p<0.05). Modelled vitamin D was 62.71 vs 57.81 nmol/L in the two groups. Time-dependent mixed-effects models quadratic in age outperformed linear-only models (p=0.0377). ODP vitamin D was shown to vary with age and to correlate with alanine aminotransferase establishing it as a biomarker of age in this group. Hepatitis C seronegativity was significant in regression models (from p=0.0015).ConclusionVitamin D was higher in ODP in both sexes in bivariate, cross-sectional, case–control and longitudinal analyses and was robust to the inclusion of metabolic and immune biomarkers. That Hepatitis C seronegativity was significant suggests opioid dependence has an effect beyond simply that of its associated hepatitides. This finding may relate to the accelerated ageing process previously described in opioid dependence.
Clinicopathological features of CD5-positive splenic marginal zone lymphoma
Authors: Li Yunling , Wang Guannan , Liu Enjie , Zhang Dandan , Zhang Yanping , Jian Xiangyu , Zhao Wugan , Li Wencai 
Published: March 2023

AimsTo investigate the clinicopathological features, immunophenotypes and differential diagnosis of CD5-positive splenic marginal zone lymphoma (SMZL).MethodsWe retrospectively analysed 16 CD5-positive cases of SMZL. Assess their clinicopathological features and survival outcomes to evaluate their similarities and differences with a control group of 25 CD5-negative cases of SMZL.ResultsCompared with CD5-negative patients, CD5-positive SMZL tends to be more prone to B symptoms, peripheral lymphadenopathy and extranodal infiltration, high Ann Arbor stage, high International Prognostic Index scores, high serum lactic dehydrogenase and high rates of bone marrow involvement. The 5-year survival rate was significantly shorter than that of the CD5-negative group (52.1% and 81.8%, respectively).ConclusionsThere are many similarities between CD5-positive SMZL and classical CD5-negative SMZL in clinical presentations, morphology and immunohistochemistry, but the former may have a more aggressive clinical course with a poorer prognosis.
Developing a Research Tool for Investigating Religious Knowledge as Part of Religious Literacy: The Questionnaire – First Results – Possibilities for International Comparisons
Authors: Schnaufer Evelyn , Rutkowski Mirjam , Räsänen Antti , Osbeck Christina , Schweitzer Friedrich 
Published: March 2023

Abstract
This article makes international debates on religious literacy as well as forms of assessment in RE related to these debates its starting point by identifying the lack of an empirical basis for the respective discussions. A questionnaire for 15-year-old pupils with a focus on religious knowledge, based on prior studies and tried out in Finland, Germany and Sweden is introduced. The results, although not representative, allow for comparative evaluation, comparing the results at country level and in reference to different kinds of religion-related knowledge. Possible influences of the different curricula are identified by comparing different degrees of familiarity with certain topics or religions in relation to when the respective curriculum foresees them being treated. The article is meant to demonstrate that meaningful international research on religious knowledge as part of religious literacy is possible, that its results could inform RE and show the possible value of developing such efforts further.
Can Rural Residential Land Transfer Raise Peasants’ Income?
Authors: Lin (林辉煌) Huihuang , Zhao (赵颖) Ying 
Published: March 2023

Abstract
In the less developed regions of China, except for cross-regional transfers to achieve horizontal poverty alleviation, which can increase peasants’ income to a certain extent in the short term, government-led rural residential land transfer does not significantly increase peasants’ income and may even reduce it. In developed regions, because peasants do not primarily depend on agriculture for their livelihoods, and because local governments have relatively ample financial resources to provide adequate compensation for rural residential land transfer, government-led rural residential land transfer can indeed raise the income of the poor to a certain extent. Essentially, the income from the transfer of rural residential land depends on the unit price and the area of rural residential land available for transfer, which should be used as the basis for determining rural residential land policies in such a way as to protect the vital interests of the greatest number of peasants.
Selective excitation of hyperbolic phonon polaritons-induced broadband absorption via α-MoO3 square pyramid arrays
Authors: Pian Chui , Sang Tian , Li Shi , Yang Chaoyu , Zhang Xianghu 
Published: March 2023

AbstractOptical anisotropy of α-MoO3 in its reststrahlen (RS) bands provides exciting opportunities for constructing the polarization-dependent devices. However, achieving broadband anisotropic absorptions through the same α-MoO3 arrays is still challenging. In this study, we demonstrate that selective broadband absorption can be achieved by using the same α-MoO3 square pyramid arrays (SPAs). For both the x and y polarizations, the absorption responses of the α-MoO3 SPAs calculated by using the effective medium theory (EMT) agreed well with those of the FDTD, indicating the excellent selective broadband absorption of the α-MoO3 SPAs are associated with the resonant hyperbolic phonon polaritons (HPhPs) modes assisted by the anisotropic gradient antireflection (AR) effect of the structure. The near-field distribution of the absorption wavelengths of the α-MoO3 SPAs shows that the magnetic-field enhancement of the lager absorption wavelength tends to shift to the bottom of the α-MoO3 SPAs due to the lateral Fabry–Pérot (F–P) resonance, and the electric-field distribution exhibits the ray-like light propagation trails due to the resonance nature of the HPhPs modes. In addition, broadband absorption of the α-MoO3 SPAs can be maintained if the width of the bottom edge of the α-MoO3 pyramid is large than 0.8 μm, and excellent anisotropic absorption performances are almost immune to the variations of the thickness of the spacer and the height of the α-MoO3 pyramid.
A holobiont approach towards polysaccharide degradation by the highly compartmentalised gut system of the soil-feeding higher termite Labiotermes labralis
Authors: Marynowska Martyna , Sillam-Dussès David , Untereiner Boris , Klimek Dominika , Goux Xavier , Gawron Piotr , Roisin Yves , Delfosse Philippe , Calusinska Magdalena 
Published: March 2023

AbstractBackgroundTermites are among the most successful insects on Earth and can feed on a broad range of organic matter at various stages of decomposition. The termite gut system is often referred to as a micro-reactor and is a complex structure consisting of several components. It includes the host, its gut microbiome and fungal gardens, in the case of fungi-growing higher termites. The digestive tract of soil-feeding higher termites is characterised by radial and axial gradients of physicochemical parameters (e.g.pH, O2and H2partial pressure), and also differs in the density and structure of residing microbial communities. Although soil-feeding termites account for 60% of the known termite species, their biomass degradation strategies are far less known compared to their wood-feeding counterparts.ResultsIn this work, we applied an integrative multi-omics approach for the first time at the holobiont level to study the highly compartmentalised gut system of the soil-feeding higher termiteLabiotermes labralis. We relied on 16S rRNA gene community profiling, metagenomics and (meta)transcriptomics to uncover the distribution of functional roles, in particular those related to carbohydrate hydrolysis, across different gut compartments and among the members of the bacterial community and the host itself. We showed that theLabiotermesgut was dominated by members of the Firmicutes phylum, whose abundance gradually decreased towards the posterior segments of the hindgut, in favour of Bacteroidetes, Proteobacteria and Verrucomicrobia. Contrary to expectations, we observed thatL. labralisgut microbes expressed a high diversity of carbohydrate active enzymes involved in cellulose and hemicelluloses degradation, making the soil-feeding termite gut a unique reservoir of lignocellulolytic enzymes with considerable biotechnological potential. We also evidenced that the host cellulases have different phylogenetic origins and structures, which is possibly translated into their different specificities towards cellulose. From an ecological perspective, we could speculate that the capacity to feed on distinct polymorphs of cellulose retained in soil might have enabled this termite species to widely colonise the different habitats of the Amazon basin.ConclusionsOur study provides interesting insights into the distribution of the hydrolytic potential of the highly compartmentalised higher termite gut. The large number of expressed enzymes targeting the different lignocellulose components make theLabiotermesworker gut a relevant lignocellulose-valorising model to mimic by biomass conversion industries.
High prevalence of cardiometabolic risk factors amongst young adults in the United Arab Emirates: the UAE Healthy Future Study
Authors: Mezhal Fatima , Oulhaj Abderrahim , Abdulle Abdishakur , AlJunaibi Abdulla , Alnaeemi Abdulla , Ahmad Amar , Leinberger-Jabari Andrea , Al Dhaheri Ayesha S. , AlZaabi Eiman , Al-Maskari Fatma , Alanouti Fatme , Alameri Fayza , Alsafar Habiba , Alblooshi Hamad , Alkaabi Juma , Wareth Laila Abdel , Aljaber Mai , Kazim Marina , Weitzman Michael , Al-Houqani Mohammad , Ali Mohammad Hag , Tuzcu E. Murat , Oumeziane Naima , El-Shahawy Omar , Al-Rifai Rami H. , Sherman Scott , Shah Syed M. , Alzaabi Thekra , Loney Tom , Almahmeed Wael , Idaghdour Youssef , Ahmed Luai A. , Ali Raghib 
Published: March 2023

Abstract
Background
Cardiovascular disease (CVD) is the leading cause of death in the world. In the United Arab Emirates (UAE), it accounts for 40% of mortality. CVD is caused by multiple cardiometabolic risk factors (CRFs) including obesity, dysglycemia, dyslipidemia, hypertension and central obesity. However, there are limited studies focusing on the CVD risk burden among young Emirati adults. This study investigates the burden of CRFs in a sample of young Emiratis, and estimates the distribution in relation to sociodemographic and behavioral determinants.

Methods
Data was used from the baseline data of the UAE Healthy Future Study volunteers. The study participants were aged 18 to 40 years. The study analysis was based on self-reported questionnaires, anthropometric and blood pressure measurements, as well as blood analysis.

Results
A total of 5167 participants were included in the analysis; 62% were males and the mean age of the sample was 25.7 years. The age-adjusted prevalence was 26.5% for obesity, 11.7% for dysglycemia, 62.7% for dyslipidemia, 22.4% for hypertension and 22.5% for central obesity. The CRFs were distributed differently when compared within social and behavioral groups. For example, obesity, dyslipidemia and central obesity in men were found higher among smokers than non-smokers (p < 0.05). And among women with lower education, all CRFs were reported significantly higher than those with higher education, except for hypertension. Most CRFs were significantly higher among men and women with positive family history of common non-communicable diseases.

Conclusions
CRFs are highly prevalent in the young Emirati adults of the UAE Healthy Future Study. The difference in CRF distribution among social and behavioral groups can be taken into account to target group-specific prevention measures.