bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of Digital Education Tools in the Pedagogical Community
Authors: Kornienko Dmitriy V. , Mishina Svetlana V. 
Published: September 2023

This article is devoted to solving the problem of accumulation, systematization, and translation of the practices of using digital educational technologies in municipal educational organizations. The solution to this problem was implemented by implementing the project “Organization of a system of digital education practices in the urban district of the City of Yelets” in 2022. The project was aimed at involving at least 350 managing and teaching staff, 4000 students in the active practice of using digital educational technologies in educational organizations of the urban district of Yelets (Lipetsk region, Russian Federation) by the end of 2022. To achieve the goal, the implementation of a system of digital education practices in educational organizations of the urban district of the city of Yelets was determined. Continuity and concession are key features of this system.
Consequently, the stakeholders are preschool educational organizations, public educational organizations, organizations of additional education, secondary vocational education, and higher education. This system involved the use of such organizational forms as a resource center, a municipal network platform, a pivotal school, and an author’s school, within which approbation, tuning, and dissemination of the experience of using software products, the LECTA digital educational platform, the digital educational environment “Mobile e-education” and directions 3D modeling, digital video and robotics. The key results of the conducted research were quantitative and qualitative indicators. The main problem that has blocked the adoption of digital education tools among the pedagogical community is the belief that they are ineffective compared to full-time education.
Use of Gamification to Approach the Algebraic Language and Equations in 1st Grade of Secondary Education
Authors: Canosa María , Sánchez Almudena , Díaz José Luis , Roa Julián 
Published: September 2023

Active methodologies, as innovative agents, are becoming more and more frequent in education, but they are not a majority. However, it is a good resource to adapt the educational system to the current society based on knowledge and information.
This article shows a useful and effective tool to connect with the needs and interests of students through an active methodology. The proposal, a gamification (teaching-learning methodology based on the mechanics of games), approaches the students of 1st grade of secondary education to the algebraic language and equations, combining cooperative learning activities.
Working through gamification means a higher academic performance, an increase in motivation and the development of a positive attitude allowing, in addition, to attend to diversity in the classroom and the use of ICT promoting collaborative work.
Gamification, based on the use of ICT, facilitates the acquisition of knowledge in a more attractive way, favoring their performance.
PERANCANGAN TATA LETAK RAK PENYIMPANAN BAHAN BAKU DI WAREHOUSE PT. BBA
Authors: Sitohang Berry Ramlan , Sumantika Arsyad 
Published: September 2023

Penelitian ini bertujuan agar dapat memberikan usulan perbaikan tata letak rak penyimpanan bahan baku pada warehouse PT BBA, sehingga dapat memangkas jarak perpindahan bahan baku dengan jarak yang lebih efektif dan efesien. Dengan mengoptimalkan perpindahan pada rancangan layout alternatif yang lebih pendek. Pada penelitian ini digunakan metode Activity Relationship Chart, dan aplikasi BLOCPLAN. Dari hasil penelitian yang dilakukan layout awal warehouse PT BBA tidak optimal oleh karena itu maka perlu dilakukan perbaikan perencanaan tata letak rak penyimpanan bahan baku dengan jarak yang lebih pendek dari segi penempatan bahan baku berdasarkan tingkat kebutuhan pada produksi. Hasil yang diperoleh dari analisis layout usulan dengan metode Activity Relationship Chart dan BLOCPLAN dapat lebih pendek dari layout sebelumnya dengan mempertimbangkan derajat kedekatan antar fasilitas. Perbandingan jarak awal 91,4m2 dan layout alternatif 78,77m2 maka didapatkan efisiensi layout alternatif 12,63m2 dan efisiensi sebesar 13,81%.
PERANCANGAN SISTEM PAKAR DENGAN METODE FORWARD CHAINING UNTUK MENDIAGNOSIS PENYAKIT DIABETES BERBASIS WEB
Authors: Sirait David Arif Setiawan , Sitohang Sunarsan 
Published: September 2023

Perkembangan komputer pada masa sekarang mengalami perubahan seiring dengan kebutuhan kehidupan manusia yang sangat semakin berkembang pesat dan kompleks. Pengetahuan tersebut telah menjangkau hampir semua kegiatan dalam aktifitas manusia yang tidak terlepas dari perangkat elektronik dalam sistem kinerja tak terkecuali pada bidang kesehatan. Bila di lihat dari aspek kehidupan, umumnya pada lingkungan masyarakat masih terdapat masyarakat yang menyepelekan atau remeh akan kesehatan dan penyakit sehingga tidak menjaga pola makan teratur dan berasumsi bahwa penyakit yang datang akan sembuh dengan sendirinya tanpa harus menemui dokter untuk melakukan pemeriksaan atau diagnosa. Selain menyepelekan penyakit sistem kegiatan mendiagnosis penyakit oleh dokter harus dilakukan secara langsung sehingga membuat bahan pertimbangan bagi masyarakat yang memerlukan waktu dan memerlukan biaya tambahan untuk melakukan pemeriksaan ke dokter. Salah satu penyakit yang sering menyerang dan sering di remehkan adalah penyakit Diabetes Melitus (DM) atau serig di sebut dengan Diabetes. Diabetes Melitus (DM) merupakan penyakit yang timbul akibat kurangnya produksi Insulin (hormon yang di produksi oleh pakreas yang mengatur tingkat glukosa) pada tubuh manusia. Penyakit ini merupakan suatu bentuk hambatan sistem kekebalan tubuh akibatnya menyebabkan peningkatan kosentrasi glukosa dalam darah (hiperglikemia) yang terbagi atas beberapa tipe. Sistem pakar merupakan suatu sistem dimana dapat menangkap dan memberikan informasi dengan menggunakan pengetahuan khusus serta metode pengambilan keputusan seperti para ahli pada bidang tertentu dan berisi tentang fakta-fakta untuk memecahkan masalah tertentu secara tepat sehingga menghasilkan keputusan sebagai kesimpulan layaknya berkonsultasi dengan seorang dokter. Forward Chaining merupakan metode yang menggunakan aturan kondisi-aksi dengan menggunakan sejumlah aturan yang dijalankan. Salah satu ciri dari metode ini ialah dengan cara melakukan penyimpanan pada media storage kerja, kemudian dialakukan pengulangan hingga menemukan hasil akhir sebagai keputusan kesimpulan. Dari penelitian yang telah dilakukan hasil yang telah dicapai adalah sebuah web yang dimana dapat berguna untuk mendiagnosis penyakit Diabetes dengan tipe 1, tipe 2 dan tipe 3.
Development of a novel clinical support tool for active surveillance of low risk papillary thyroid cancer
Authors: White Eleanor , Abbott Bridget , Schembri Geoffrey , Glover Anthony , Clifton-Bligh Roderick , Gild Matti L. 
Published: September 2023

BackgroundActive surveillance (AS) is an alternative to surgery in select patients with very low risk papillary thyroid cancer (PTC). Many clinicians feel ill-equipped in selecting appropriate patients. We aimed to 1) Develop an evidence-based web delivered decision support tool to assist clinicians in identifying patients appropriate for AS; and 2) Evaluate the prevalence of patients suitable for AS in a tertiary high volume thyroid cancer centre.MethodA REDCap web based clinical support tool was developed utilising evidence-based characteristics for AS suitability available to clinicals during initial assessment. A retrospective database was interrogated for patients who underwent hemithyroidectomy between 2012 – 2021 with final histopathology demonstrating PTC. Patients with PTCs&gt;2cm, missing data, benign disease on surgical histopathology or incidental PTC were excluded.ResultsBetween 2012 - 2021, 763 patients underwent hemithyroidectomy with final histopathology confirming PTC. Of these, 316 patients were excluded (missing data, incidental PTC, concomitant hyperparathyroidism were most common reasons for exclusion) and 114/447 remaining patients had a pre-operative fine needle aspirate (FNA) of Bethesda V or VI (high likelihood of malignancy). Using the tool, 59/114 (52%) met criteria for AS. The majority of patients were female (85% vs 15% male); median age 36 years (range 19 – 78). Following initial surgery, 10/59 patients had a completion thyroidectomy, with 4/10 demonstrating malignancy in contralateral lobe and eight of those patients undergoing I131 ablation. During a median follow up of over 3 years, 49/59 (83%) did not require further surgery or intervention with no patients developing recurrence. A subgroup analysis with second radiology assessment excluded 4/59 patients as meeting criteria for AS based on presence of ETE on preoperative ultrasound. None of these 4 patients had completion thyroidectomy.ConclusionOur clinical support tool identifies patients with PTC potentially suitable for AS which could be utilised during initial patient assessment. In a retrospective cohort of patients who had hemithyroidectomy for PTC with a pre-operative FNA diagnosis of Bethesda V or VI, 55/114 (48%) patients may have been suitable for AS. Prospective validation studies are required for implementation of the tool in clinical practice.
The association between body mass index and live birth and maternal and perinatal outcomes after in-vitro fertilization: a national cohort study
Authors: Kluge Linda , Källén Karin , Thurin-Kjellberg Ann , Wennerholm Ulla-Britt , Bergh Christina 
Published: September 2023

ObjectiveTo investigate the association between female body mass index (BMI) and live birth rates and maternal and perinatal outcomes after in-vitro fertilization (IVF).MethodsWe performed a national, population-based cohort study including women undergoing IVF between 2002 and 2020. The cohort included 126,620 fresh cycles and subsequent frozen embryo transfers between 2007 and 2019 (subpopulation 1) and 58,187 singleton deliveries between 2002 and 2020 (subpopulation 2). Exposure was female BMI (kg/m2) categorized according to the World Health Organization as underweight (&lt;18.5), normal weight (18.5–24.9, reference), overweight (25.0–29.9), class I obesity (30.0–34.9), class II obesity (35.0–39.9), and class III obesity (≥40.0). The primary outcome in subpopulation 1 was cumulative live birth per started fresh IVF cycle, including fresh and subsequent frozen embryo transfers. Primary outcomes in subpopulation 2 were hypertensive disorders of pregnancy and preterm birth at less than 37 weeks. Risk ratios (RRs) with 95% confidence intervals (CIs) for the association between BMI class and outcomes were calculated using generalized linear models after adjustment for relevant confounders.ResultsThe cumulative live birth rate decreased significantly with increasing BMI from 32.6% in normal-weight women to 29.4% in overweight women, 27.0% in women in obesity class I, 21.8% in women in obesity class II, and 7.6% in women in obesity class III. The risk of hypertensive disorders of pregnancy increased significantly and progressively with increasing BMI, from 4.6% in normal-weight women to 7.8% in overweight women and 12.5%, 17.9%, and 20.3% in women in obesity classes I, II, and III. The risk of preterm birth followed a similar pattern, from 6.3% in normal-weight women to 7.5% in overweight women and 8.9%, 9.9%, and 15.3% in women in obesity classes I, II, and III. The risks of other perinatal complications, such as perinatal death, showed an even more pronounced increase.ConclusionUsing a large and complete national cohort of women undergoing IVF, we demonstrate a dose-dependent decrease in live birth rate and a substantial increase in maternal and perinatal complications with increasing BMI. Strategies to improve this situation are warranted.
Novel ceRNA network construction associated with programmed cell death in acute rejection of heart allograft in mice
Authors: Guo Yiwen , Zhang Yixi , Yu Jia , Dong Yuqi , Chen Zhitao , Zhu Chuchen , Hong Xitao , Xie Zhonghao , Zhang Min , Wang Shuai , Liang Yichen , He Xiaoshun , Ju Weiqiang , Chen Maogen 
Published: September 2023

BackgroundT cell-mediated acute rejection(AR) after heart transplantation(HT) ultimately results in graft failure and is a common indication for secondary transplantation. It’s a serious threat to heart transplant recipients. This study aimed to explore the novel lncRNA-miRNA-mRNA networks that contributed to AR in a mouse heart transplantation model.MethodsThe donor heart from Babl/C mice was transplanted to C57BL/6 mice with heterotopic implantation to the abdominal cavity. The control group was syngeneic heart transplantation with the same kind of mice donor. The whole-transcriptome sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in mouse heart allograft. The biological functions of ceRNA networks was analyzed by GO and KEGG enrichment. Differentially expressed ceRNA involved in programmed cell death were further verified with qRT-PCR testing.ResultsLots of DEmRNAs, DEmiRNAs and DElncRNAs were identified in acute rejection and control after heart transplantation, including up-regulated 4754 DEmRNAs, 1634 DElncRNAs, 182 DEmiRNAs, and down-regulated 4365 DEmRNAs, 1761 DElncRNAs, 132 DEmiRNAs. Based on the ceRNA theory, lncRNA-miRNA-mRNA regulatory networks were constructed in allograft acute rejection response. The functional enrichment analysis indicate that the down-regulated mRNAs are mainly involved in cardiac muscle cell contraction, potassium channel activity, etc. and the up-regulated mRNAs are mainly involved in T cell differentiation and mononuclear cell migration, etc. The KEGG pathway enrichment analysis showed that the down-regulated DEmRNAs were mainly enriched in adrenergic signaling, axon guidance, calcium signaling pathway, etc. The up-regulated DEmRNAs were enriched in the adhesion function, chemokine signaling pathway, apoptosis, etc. Four lncRNA-mediated ceRNA regulatory pathways, Pvt1/miR-30c-5p/Pdgfc, 1700071M16Rik/miR-145a-3p/Pdgfc, 1700071M16Rik/miR-145a-3p/Tox, 1700071M16Rik/miR-145a-3p/Themis2, were finally validated. In addition, increased expression of PVT1, 1700071M16Rik, Tox and Themis2 may be considered as potential diagnostic gene biomarkers in AR.ConclusionWe speculated that Pvt1/miR-30c-5p/Pdgfc, 1700071M16Rik/miR-145a-3p/Pdgfc, 1700071M16Rik/miR-145a-3p/Tox and 1700071M16Rik/miR-145a-3p/Themis2 interaction pairs may serve as potential biomarkers in AR after HT.
Biomass Identification from Proximate Analysis: Characterization of Residual Vegetable Materials in Andean Areas
Authors: Velázquez Martí Borja , Gaibor-Chávez Juan , Franco Rodríguez John Eloy , López Cortés Isabel 
Published: September 2023

This work was aimed at the characterization of residual generated biomass from pruned tree species present in the Andean areas of Ecuador as a source of energy, both in plantations and in urban areas, as a response to the change in the energy matrix proposed by the Ecuadorian government. From the proximate analysis (volatiles, ashes, and fixed carbon content), elemental analysis (C, H, N, S, O, and Cl), structural analysis (cellulose, lignin, and hemicellulose content), and higher heating value, the studied species were pine (Pinus radiata), cypress (Cupressus macrocarpa), eucalyptus (Eucalyptus globulus), poplar (Populus sp.), arupo (Chionanthus pubescens), alder (Alnus Acuminata), caper spurge (Euphorbia laurifolia), and lime (Sambucus nigra L.) trees. We evaluated the influence of the presence of leaves in the biomass. From this characterization, we developed a method based on obtaining the main components for the identification of the biomass’s species. If the origin of the biomass was unknown, this method enabled us to identify the species, with all its characteristics. If the origin of the biomass was unknown, this innovative method enabled the identification of the species from the lignocellulosic biomass, with all of its characteristics. Finally, we developed regression models that relate the higher heating value to the elemental, proximate, and structural composition.
In Vitro Impact of Fluconazole on Oral Microbial Communities, Bacterial Growth, and Biofilm Formation
Authors: Dornelas-Figueira Louise Morais , Ricomini Filho Antônio Pedro , Junges Roger , Åmdal Heidi Aarø , Cury Altair Antoninha Del Bel , Petersen Fernanda Cristina 
Published: September 2023

Antifungal agents are widely used to specifically eliminate infections by fungal pathogens. However, the specificity of antifungal agents has been challenged by a few studies demonstrating antibacterial inhibitory effects against Mycobacteria and Streptomyces species. Here, we evaluated for the first time the potential effect of fluconazole, the most clinically used antifungal agent, on a human oral microbiota biofilm model. The results showed that biofilm viability on blood and mitis salivarius agar media was increased over time in the presence of fluconazole at clinically relevant concentrations, despite a reduction in biomass. Targeted PCR revealed a higher abundance of Veillonella atypica, Veillonella dispar, and Lactobacillus spp. in the fluconazole-treated samples compared to the control, while Fusobacterium nucleatum was reduced and Streptococcus spp were not significantly affected. Further, we tested the potential impact of fluconazole using single-species models. Our results, using Streptococcus mutans and Streptococcus mitis luciferase reporters, showed that S. mutans planktonic growth was not significantly affected by fluconazole, whereas for S. mitis, planktonic growth, but not biofilm viability, was inhibited at the highest concentration. Fluconazole’s effects on S. mitis biofilm biomass were concentration and time dependent. Exposure for 48 h to the highest concentration of fluconazole was associated with S. mitis biofilms with the most increased biomass. Potential growth inhibitory effects were further tested using four non-streptococcal species. Among these, the planktonic growth of both Escherichia coli and Granulicatella adiacens was inhibited by fluconazole. The data indicate bacterial responses to fluconazole that extend to a broader range of bacterial species than previously anticipated from the literature, with the potential to disturb biofilm communities.
The Molecular Epidemiology of Clostridioides difficile Infection in Central India: A Prospective Observational Cohort Study
Authors: Biswas Rima , Pinkham Nick , Walk Seth T. , Wang Qian , Ambalkar Shrikant , Satav Ashish R. , Wilcox Mark H. , Reghunath Rahul , Chawla Kiran , Shenoy Padmaja A. , Nayak Amit R. , Husain Aliabbas A. , Raje Dhananjay V. , Kashyap Rajpal Singh , Monaghan Tanya M. 
Published: September 2023

This prospective observational cohort study aimed to establish and compare baseline rates of Clostridioides difficile infection (CDI) in community and hospitalized patients in Nagpur and rural Melghat Maharashtra, including adults aged ≥18 years with a diagnosis of diarrhoea as defined as 3 or more loose stools in a 24 h period. All diarrhoeal samples were tested for CDI using the C. diff Quik Chek Complete enzyme immunoassay. C. difficile-positive stool samples were characterised by toxigenic culture, antimicrobial susceptibility testing and PCR ribotyping. C. difficile testing was performed on 1683 patients with acute diarrhoea. A total of 54 patients (3.21%; 95% CI: 2.42–4.17) tested positive for both the GDH antigen and free toxin. The risk factors for CDI included the presence of co-morbidities, antibiotic usage, and immunosuppression. The detected PCR ribotypes included 053-16, 017, 313, 001, 107, and 216. Our findings show that toxigenic C. difficile is an important but neglected aetiologic agent of infective diarrhoea in Central India. These results underscore the need to enhance the awareness and testing of patients with diarrhoea in India regarding the presence of toxigenic C. difficile, particularly in high-risk individuals with multiple co-morbidities, immunosuppression, and recent or ongoing antibiotic exposure or hospitalization.
Recombinant Attenuated Edwardsiella piscicida Vaccine Displaying Regulated Lysis to Confer Biological Containment and Protect Catfish against Edwardsiellosis
Authors: Swain Banikalyan , Campodonico Vanessa A. , Curtiss Roy 
Published: September 2023

We implemented a unique strategy to construct a recombinant attenuated Edwardsiella vaccine (RAEV) with a biological containment phenotype that causes regulated bacterial cell wall lysis. This process ensures that the vaccine strain is not able to persist in the environment. The murA gene is responsible for the catalysis of one of the first steps in the biosynthesis of muramic acid, which is a crucial component of the bacterial cell wall. The regulated lysis phenotype was achieved by inserting the tightly regulated araC ParaBAD cassette in place of the chromosomal murA promoter. Strains with this mutation require growth media supplemented with arabinose in order to survive. Without arabinose, they are unable to synthesize the peptidoglycan cell wall. Following the colonization of fish lymphoid tissues, the murA protein is no longer synthesized due to the lack of arabinose. Lysis is subsequently achieved in vivo, thus preventing the generation of disease symptoms and the spread of the strain into the environment. Vaccine strain χ16016 with the genotype ΔPmurA180::TT araC ParaBAD murA is attenuated and shows a higher LD50 value than that of the wild-type strain. Studies have demonstrated that χ16016 induced TLR4, TLR5, TLR8, TLR9, NOD1 and NOD2-mediated NF-κB pathways and upregulated the gene expression of various cytokines, such as il-8, il-1β, tnf-a, il-6 and ifn-γ in catfish. We observed significant upregulation of the expression profiles of cd4, cd8 and mhc-II genes in different organs of vaccinated catfish. Vaccine strain χ16016 induced systemic and mucosal IgM titers and conferred significant protection to catfish against E. piscicida wild-type challenge. Our lysis RAEV is the first live attenuated vaccine candidate designed to be used in the aquaculture industry that displays this biological containment property.
As potencialidades e fragilidades de um projeto na vertente da educação ambiental patrimonial
Authors: Ricetto Gabriela Loureiro Martins , Campos Marília Andrade Torales 
Published: September 2023

RESUMO
Ao descrever o desenvolvimento de um projeto multi/interdisciplinar no campo da Educação Ambiental e da Preservação do Patrimônio, neste artigo se apresentam os resultados de uma pesquisa, fulgurando as potencialidades e fragilidades no processo de elaboração e desenvolvimento do projeto intitulado “Caminhos das Ferrovias, Natureza e Cultura”. A proposta foi idealizada por 10 professores do Novo Ensino Médio (NEM), dos componentes curriculares de Arte, Geografia, História e Inglês de uma escola pública estadual de Curitiba (Paraná). Assim, a metodologia adotada pelo estudo em questão, valeu-se da ótica dos professores, com base na pesquisa participante, aplicação de questionário e da Análise de Conteúdo. Os resultados apontam que os professores ressaltaram mais as potencialidades relacionadas ao projeto do que os aspectos frágeis, o que indica que a experiência possui potencial de replicação e possível continuidade de iniciativas semelhantes. Tal resultado reforça que a prática efetiva e contínua da Educação Ambiental no contexto escolar, por meio de projetos pedagógicos coletivos, poderia ser uma alternativa que se soma a outras para auxiliar o processo de inserção ou potencialização da Educação Ambiental nas escolas.
Palavras-chave: Educação Ambiental Patrimonial, Projeto Pedagógico, Potencialidades e Fragilidades.