bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of sex on the immune microenvironment in male and female breast cancer
Authors: Hargrove-Wiley Ebony , Fingleton Barbara 
Published: August 2023

Abstract
Even when accounting for multiple clinical parameters, male breast cancer patients have significantly lower survival rates than females. Males and females differ in their levels of sex hormones androgens and estrogens. These sex hormones have varying influences on immune cell populations, which in turn may affect disease progression. Thus, the sex of the patient and the levels of sex hormones are likely important for anticancer immunity. Here, we assessed sex differences in the immune landscape of primary and metastatic tumors. We used flow cytometry to determine the immune profile of mammary tumors, spleens, and lungs, and histochemistry to evaluate metastasis in transgenic MMTV-PyMT and in mice orthotopically injected with sex-specific MMTV-PyMT-derived cell lines in male and female cohorts. In transgenic mice, males had greater proportions of CD4+ T-helper and total myeloid cells in all organs analyzed. The splenic and pulmonary immune profiles of tumor-bearing males were more similar to cancer-free littermates than in females. Interestingly, males trended towards increased metastatic colonization in the lungs. Sex-associated immune profiles differed between transgenic and orthotopic mice. In mice bearing male-derived R221a PyMT tumors, females had decreased intratumoral neutrophils and elevated natural killer cells with increased splenic B-cells in comparison to males. Overall, our data supports a sex-dependent immune response in mammary carcinoma that is, in part, regulated by the hormonal environment of the host. With emerging therapeutics targeting the tumor immune microenvironment, characterization of immune profiles is critical for optimizing their use in all breast cancer patients.
Supported by grants from METAvivor Research and Support Inc. and the National Science Foundation Graduate Research Fellowship Program under Grant No. 1937963.
The role of Th2 in immune surveillance of NAFLD-associated liver metastasis
Authors: Chan Ting Hei Thomas , Tang Wenshu , Zhou Jingying 
Published: August 2023

Abstract
Non-alcoholic fatty liver disease (NAFLD) is an increasing global epidemic associated with chronic inflammation, metabolic disorders, immunosuppression and importantly, increased incidence of primary and metastatic liver cancers. We have recently demonstrated that the immunosuppressive nature of NAFLD livers represented by increased myeloid-derived suppressor cells and dysregulated natural-killer T cells, resulted in aggressive tumorigenesis and immunotherapy resistance of primary liver cancer. Given the importance of metabolic and immune imbalance in the formation of metastatic-prone liver microenvironment, we continued to dissect the cellular complexity of NAFLD livers in metastatic liver cancers. Through bulk and single-cell RNA-sequencing (scRNA-seq) dataset analysis from mice and patients with NAFLD or liver metastasis (LM), we found that the CD4+ T helper-2 (Th2) population and the regulon activity of its master transcription factor GATA3 were also remarkably reduced in NAFLD or LM compared to healthy controls. Interestingly, increased hepatic metastasis was observed in mice with NAFLD induced by high-fed-high-carbohydrate-diet compared to controlled mice by orthotopic LM mouse models, accompanied with reduced hepatic Th2 cells which negatively correlated with increased triglyceride and cholesterol levels. Taken together, our preliminary findings suggested a potential immunosurveillance role of hepatic Th2 cells that may be dysregulated by lipid metabolic alterations in NAFLD. Therapeutic approaches to restore hepatic Th2 cells may therefore provide new perspective and therapeutic approaches to reduce liver metastasis in the era of NAFLD epidemic. The project is supported by RGC/GRF14104820.
The project is supported by RGC/GRF14104820.
A novel ion channel-mediated mechanism facilitates the inhibitory effect of exosomes on anti-tumor immune responses
Authors: Chimote Ameet A , Alshwimi Abdulaziz O , Bhati Jay , Langevin Scott M , Wise-Draper Trisha M , Khodoun Marat V , Conforti Laura 
Published: August 2023

Abstract
Head and neck squamous cell carcinomas (HNSCC) are aggressive cancers that are associated with high morbidity and mortality. Anti-tumor immune responses rely on the ability of cytotoxic T and NK cells to infiltrate the solid tumor microenvironment and kill the cancer cells. However, solid tumors utilize multiple mechanisms to evade these cells including the production of exosomes, which inhibit immune cell function and present a major block to immunotherapy. The mechanisms by which the tumor-derived exosomes (TEX) inhibit immune cell functionality are poorly understood. We isolated TEX from a HNSCC patient tumor-derived primary cancer cell line by ultracentrifugation and characterized them by electron microscopy and flow cytometry. We exposed healthy donor (HD) peripheral blood mononuclear cells (PBMCs) to TEX, followed by activation, and conducted preliminary single-cell studies by measuring transcriptome (NanoString), secretome of 32 secreted proteins (Isoplexis) and immune cell function (electrophysiology). Transcriptomic studies revealed that TEX reduced the abundance of cytotoxic cells, and inhibited immune responses associated with cytotoxicity, chemokines, and NK and T cell functions. In CD8+ T and NK cells, TEX reduced polyfunctionality (the ability to secrete multiple cytokines from a single cell). ELISA showed that TEX reduced IFN-γ secretion by CD8+ T and NK cells. KCa3.1 channels regulate the Ca2+ signaling necessary for these immune effector functions, but the effect of TEX on KCa3.1 activity is not known. Electrophysiology revealed that TEX inhibited KCa3.1 channel activity in HD CD8+ T cells. Overall, our data suggest that KCa3.1 channels mediate the inhibitory effect of TEX on anti-tumor immunity.
Bridge funds by the Dept of Internal Medicine, University of Cincinnati to Dr. Laura Conforti Pilot grant from the University of Cincinnati Cancer Center to Dr. Laura Conforti
Lung Adenocarcinoma Expression of the Aryl Hydrocarbon Receptor Promotes PD-L1 and Immune Suppression
Authors: Snyder Megan , Lara Brian , Wang Zhongyan , Fimbres Jocelyn , Khan Mohammed Muzamil , Song Gang , Mazzilli Sarah A , Monti Stefano , Sherr David 
Published: August 2023

Abstract
Immunotherapy has shown dramatic results in treating cancer, but only a minority of patients benefit. Therefore, there is an unmet medical need to understand the regulation of immune suppression in tumors. We show that the aryl hydrocarbon receptor (AhR) is a central player in regulating immune checkpoints in a mouse model of lung adenocarcinoma (LUAD). Within the murine malignant cell, AhR regulates expression of several genes important to suppressive immune signaling, including PD-L1. We find similar immune-related regulatory mechanisms in human LUAD cells. Notably, the presence of IFNγ boosts the expression of these AhR-regulated suppressive genes, suggesting a deleterious role for IFNγ in LUAD. Transplantation of mouse AhR-knockout CMT167 (CMT167AhR-KO) LUAD cells leads to partial or complete tumor rejection and protection from wild type (WT) LUAD challenge. CMT167AhR-KO tumors that do form have decreased PD-L1 expression and increased T cell infiltration relative to AhR+ control tumors. To identify specific immune cell subsets involved in AhR-related tumor immunity, we employed an imaging mass cytometry (IMC) platform coupled with single cell RNA sequencing of tumor infiltrating CD45+ immune cells. Understanding the phenotypes and spatial orientation of the immune cells interacting with malignant cells will provide evidence of the key immune populations responsible for anti-tumor immunity and thus help pave the way for future therapies and biomarkers relevant to improving treatments for LUAD patients.
Find the Cause Consortium Gifts
Molecular regulation of CD8 T cell exhaustion in a mouse model of acute lymphocytic leukemia
Authors: Gomez Amber , Green William D , Green Jarred M , Baldwin Albert , Milner Justin 
Published: August 2023

Abstract
Acute lymphocytic leukemia (ALL) is a rapidly progressive form of leukemia more commonly found in children. Individuals nonresponsive to or ineligible for traditional chemotherapy and allogeneic hematopoietic cell transplant turn to alternatives like immunotherapies. While promising, current FDA approved immunotherapies for ALL have varying efficacy rates. CD8 T cell dysfunction or exhaustion is a key contributor to immunotherapy resistance, yet the molecular mechanisms controlling CD8 T cell exhaustion in ALL remain unclear. Here we sought to define those mechanisms in a mouse model of ALL. We utilized a syngeneic B cell acute lymphocytic leukemia mouse model (B-ALL: Arf−/− p185+ BCR-ABL+ pro-B cells) to profile the distribution and molecular features of B-ALL specific CD8 T cells across multiple tissue sites and disease stages. We also compared tumor-specific CD8 T cell responses across B-ALL and solid tumor models to define both conserved and B-ALL specific phenotypes. Our findings indicate that B-ALL specific CD8 T cells share conserved features of exhausted CD8s, but also exhibit exhaustion-features unique to B-ALL. Ongoing experiments seek to define the transcriptional and epigenetic mechanisms guiding B-ALL specific CD8 T cell exhaustion, which may facilitate adoptive cell therapy strategies to improve CD8 T cell function and immunotherapy efficacy.
Supported by a grant from NIH (T32 GM133364)
Simple eigenvalues of cubic vertex-transitive graphs
Authors: Guo Krystal , Mohar Bojan 
Published: August 2023

AbstractIf
${\mathbf v} \in {\mathbb R}^{V(X)}$
is an eigenvector for eigenvalue
$\lambda $
of a graph X and
$\alpha $
is an automorphism of X, then
$\alpha ({\mathbf v})$
is also an eigenvector for
$\lambda $
. Thus, it is rather exceptional for an eigenvalue of a vertex-transitive graph to have multiplicity one. We study cubic vertex-transitive graphs with a nontrivial simple eigenvalue, and discover remarkable connections to arc-transitivity, regular maps, and number theory.
MONITORING OF THE RIVERS OF THE BLACK SEA BASIN FLOWING THROUGH THE SETTLEMENTS OF THE ADJARA REGION
Authors: Darchidze Guram 
Published: August 2023

Guram Darchidze
Email:darchidz1995@gmail.com
Ph.D. student, Georgian Technical University
Tbilisi, Georgia
https://orcid.org/0000-0002-7597-856X
Abstract. The problem of water pollution is becoming more and more urgent for the world. Despite the implemented measures, improvement of wastewater treatment methods, the issue remains relevant. One of the most common is biological pollution, directly related to the entry of pathogenic microorganisms, bacteria, viruses and protozoa into water.
The effects of water pollutants on specific waters vary and may not always be fully appreciated. However, we know these pollutants harm drinking water supplies, recreation, fisheries, and wildlife. That is why it is necessary to find the source of biological water pollution, to study its age and lethality, to develop an effective method of pollution elimination.
The topic "Monitoring of the rivers of the Black Sea basin flowing through the settlements of the Adjara region" presents the areas of human activity of large and small rivers in the Adjara region and the research and evaluation of the pollution caused by them.
For the process of development of the topic, an initial study was carried out on more than 300 large and small rivers flowing in Adjara territory. They were studied and evaluated in terms of biological contamination.
16 rivers were selected, on which a bacteriological study was carried out to determine the presence of fecal waste, discharge, turbidity and E-Coli (in field conditions) were determined on other sources.
In the report, I have presented the following works:

Evaluation of rivers at the stage of the primary study, assessment of type, discharge, turbidity, E.Coli and other types of anthropogenic pollution. 2. Laboratory research conducted to determine the level of contamination with fecal waste; 3. After determining the level of pollution, the search for centers of pollution was carried out; 4. After finding the foci, evaluating these foci and determining the probable age of the future pollution capacity; 5. After the evaluation of the pollution centers, recommendations for the elimination of pollution were developed, both informational, constructive, and biological/chemical. 6. According to the data obtained in the research, a histogram was constructed and the data were grouped according to the optimal number of intervals; 7. According to the data obtained in the research, a graph of dependence on the debit of pollution with fecal waste was constructed.

Conclusions are drawn, including the implementation of work to eliminate the problem of the existing situation.
IMPORTANCE OF FINANCIAL SERVICES’ TRANSPARENCY AND THE FINANCIAL EDUCATION FOR THE PROTECTION OF CONSUMER RIGHTS IN GEORGIA
Authors: Gavtadze George , Iphshiradze Aza 
Published: August 2023

George Gavtadze
Email:george.gavtadze@atsu.edu.ge
Professor, Akaki Tsereteli State University
Kutaisi, Georgia
https://orcid.org/0000-0001-6080-0163
Aza Iphshiradze
Email:a.iphshirdze@unik.edu.ge
Professor, Kutaisi University
Kutaisi, Georgia
https://orcid.org/0009-0005-8373-3043

Abstract. The issues of financial services’ transparency, consumer rights protection, financial education are relevant and the feasibility of their study is determined by the features of social-economic development. To overcome the problem, to some extent, it is possible to create and develop regulatory schemes for the protection of consumer rights. As research shows, priorities and practical implementation of consumer rights protection differ from country to country. The regulation is mainly aimed at providing complete information about the product to the consumer. However, in addition to imperative regulatory norms, in developed countries, the existence of self-regulation mechanisms, such as codes of conduct, is also of great importance.
Despite the adoption of a number of laws and legislative acts, creation of regulatory documents, etc., in terms of further improvement of consumer rights protection, at the modern stage, the greatest emphasis is still placed on financial education and raising consumer awareness. Consumer rights protection and financial education are two sides of the same coin and serve the same purpose.
For more than a decade, many countries have been implementing projects aimed at raising the level of financial education, which, for the most part, is manifested in the development of a national strategy for financial education. It should be noted that the need to develop a national strategy is often coupled with consumer financial protection and financial inclusion measures. At the same time, it should take into account and be adapted to the needs and shortcomings of a specific country.
As a result of the analysis of the current situation, we can conclude that the improvement of financial services, the protection of consumer rights and their financial education can bring significant benefits not only to individuals or groups of people, but also to the country as a whole (both developed and developing).
Keselarasan Iringan dan Gerak Tari Tapel Telu Sanggar Gedeng Kedaton Lendang Nangka Lombok Timur
Authors: Markarma Muh. Ridwan , Zaiful Zaiful , Huliazati Eka 
Published: August 2023

Tujuan penelitian ini adalah untuk mengetahui keselarasan iringan dan gerak tari kreasi Tapel Telu di Sanggar Gedeng Kedaton Lendang Nangka Kecamatan Masbagik. Dengan menggunakan teori keselarasan, iringan musik, dan gerak tari. Metode yang digunakan pada penelitian ini menggunakan metode kualitatif dengan pendekatan etnografiskarena mengkaji dalam bentuk budaya yaitu kesenian gamelan, tari, dan pesan moral dalam tari adalah pesan budaya. Karena memaparkan tentang keselarasan iringan dan gerak tari kreasi Tapel Telu di Sanggar Gedeng Kedaton Lendang Nangka Kecamatan Masbagik. Teknik pengumpulan data yaitu menggunakan observasi, wawancara dan pengumpulan data dengan dokumentasi. Hasil penelitian menunjukkan bahwa sebagian besar iringan dan gerak tari kreasi Tapel Telu ini selaras baik dari makna atau dari ketukan iringan dan gerakan, namun ada beberapa diantara aspek gerak dan iringan tidak selarasyang mengharuskan tarian ini digubah kembali agar terdapat kesesuain antara iringan dan gerak.
Interventions for Social Participation for Autistic Adults (2013–2020)
Authors: Jirikowic Tracy , Ideishi Roger , Bendixen Roxanna , Pfeiffer Beth , Smythe Robert , Benevides Teal 
Published: August 2023

AbstractSystematic Review Briefs provide a summary of the findings from systematic reviews developed in conjunction with the American Occupational Therapy Association’s Evidence-Based Practice Program. Each Systematic Review Brief summarizes the evidence on a theme related to a systematic review topic. This Systematic Review Brief presents findings on social participation interventions and participation outcomes for autistic1 adults.
LEARN TO SPEAK ENGLISH SOFTWARE THE PERSPECTIVE AND RESPONSE (AN EXPERIMENTAL STUDY AT POLITEKNIK LP3I MAKASSAR)
Authors: Trista Udin Imran 
Published: August 2023

The objectives of this research were: to find out whether) to find out whether or not the students are interested in joining the speaking class through Learn to Speak English software. This research employed Quasi Experimental design. The sample consisted of 32 students of third semester of business administration, Politeknik LP3I Makassar in academic year 2019/2020. The data were collected through and data on the students’ interest were analyzed using Likert scale, the results of the research were: the use of Learn to Speak English software increased the students’ interest in joining the speaking class, it can be concluded the students have high interest and active toward teaching speaking through Learn to Speak English software.
Integrasi Pendidikan Karakter pada Pembelajaran IPS Terpadu dalam berperilaku siswa kelas IV SD Inpres Padang Lampe
Authors: Muliana Muliana , Basri Muh. , Ristiana Evi 
Published: August 2023

Tujuan penelitian adalah untuk mengetahui Integrasi Pendidikan Karakter pada Pembelajaran IPS Terpadu dalam berperilaku siswa. Penelitian menggunakan deskriptif dengan pendekatan metode penelitian kualitatif. Penelitian ini dilaksanakan di SD Inpres Padang Lampe pada bulan Maret-April 2021. Informan dalam penelitian ini adalah kepala sekolah, guru kelas, 5 orang siswa kelas IV, dan 2 orang tua siswa yang menjadi informan. Metode pengumpulan data menggunakan wawancara, Observasi, dan dokumentasi. Teknik analisis menggunakan analisis kualitatif. Hasil penelitian menunjukkan bahwa implementasi pendidikan karakter terdiri atas dua komponen dalam hal (1) perencanaan pembelajaran tematik dari guru kelas IV di SD cenderung sudah menunjukkan adanya pengintegrasian pendidikan karakter dan bentuk implementasi pendidikan karakter pada pembelajaran IPS Terpadu, dapat dilihat dari pengintegrasian dalam setiap kegiatan pembelajaran dan metode pembelajaran. (2) pelaksanaan mengintegrasikan pendidikan karakter melalui program pengembangan diri yaitu kegiatan spontan. Guru menggunakan strategi pendidikan karakter yang digunakan oleh guru melalui sikap-sikap, yaitu menciptakan suasana yang kondusif dan penanaman kedisiplinan. Faktor pendukung yaitu sekolah telah menyediakan perpustakaan sebagai sarana bagi siswa untuk menggali ilmu pengetahuan secara mendalam, dan sarana bagi guru untuk kreatif dalam menggunakan berbagai sumber belajar seperti lingkungan sosial, media elektronik maupun cetak. Kendala yang terjadi bukan hanya berasal pada diri siswa sendiri, melainkan lingkungan sekitar juga seperti keluarga, sekolah, masyarakat dan media elektronik maupun cetak dapat mempengaruhi perilaku siswa.
Sistem Pendukung Keputusan Dalam Penentuan Penerima Bantuan Alat Peternakan Menggunakan Metode MOORA
Authors: Pangestu Sidik , Nugroho Nurcahyo Budi , Mahyuni Rina 
Published: August 2023

Meningkatnya jumlah permintaan hewan ternak mengakibatkan banyaknya kelompok peternak yang mengajukan permohonan bantuan alat peternakan untuk meningkatkan produktivitas ternaknya kepada Dinas Ketahanan dan Peternakan yang menyebabkan pihak Dinas Ketahanan Pangan dan Peternakan harus menyeleksi kelompok peternakan yang layak untuk di beri bantuan alat peternakan. Namun sayang proses penentuan penerima bantuan alat peternakan masih dilakukan secara manual sehingga pada saat penentuan penerima bantuan alat peternakan sering salah sasaran dan kurangnya efisien dan efektifitasnya.Untuk membantu agar proses penentuan penerima bantuan alat peternakan yang lebih baik kedepannya, maka dibutuhkan sebuah Sistem Pendukung Keputusan yang merupakan sistem berbasis komputer sehingga dapat membantu mendukung sebuah keputusan yang dihasilkan melalui dukungan metode MOORA dengan menentukan nilai terbesar, maka prioritas penerima bantuan alat peternakan yang paling tinggi akan didapat.Berdasarkan hasil dari penelitian ini, Sistem Pendukung Keputusan berhasil dibangun guna membantu masalah untuk menentukan penerima bantuan alat peternakan pada Dinas Ketahanan Pangan dan Peternakan. sehingga dapat menentukan kelompok peternakan yang lebih diutamakan mendapatkan alat peternakan.Kata Kunci: Bantuan, Alat Peternakan, Sistem Pendukung Keputusan, MOORA, Peternakan
Implementasi RSA Untuk Enkripsi Dan Dekripsi File Dokumen
Authors: Marpaung Arnold , Ramadhan Puji Sari , Pranata Ardianto 
Published: August 2023

Abstrak File dokumen adalah kumpulan data atau informasi tertentu yang tersimpan didalam berkas sistem yang dapat diakses oleh pengguna yang memiliki akses tersebut.File dokumen digunakan sebagai sarana untuk saling menerima dan berbagi informasi baik dari satu orang kebeberapa orang lainnya. Data atau informasi yang terdapat didalam file dokumen sering berisi data yang penting atau bahkan yang sifatnya rahasia.Oleh karena itu perlunya adanya pengamanan data atau informasi yang terdapat didalam file dokumen tersebut dengan menggunakan kriptografi. Kriptografi adalah ilmu atau seni untuk menjaga pesan dengan cara menyandikannya kedalam bentuk yang tidak dapat dimengerti lagi maknanya.Salah satu algoritma kriptografi adalah algoritma kriptografi RSA.Algoritma RSA merupakan salah satu algoritma Asimetris,yang mana menggunakan dua buah kunci yang berbeda untuk proses enkripsi dan dekripsinya.Algoritma RSA termasuk salah satu algoritma yang paling maju dalam dunia kriptografi. Algoritma ini memiliki keamanan yang terletak pada sulitnya memfaktorkan bilangan besar menjadi bilangan faktor-faktor prima,untuk mendapatkan kunci public dan kunci privat.Semakin sulit menentukan bilangannya maka semakin kuat algoritma RSA. Hasil yang diperoleh dari proses pengamanan data atau informasi yang terdapat didalam file dokumen menggunakan algoritma RSA yaitu pengguna dapat mengenkripsi dan mengdekripsi file dokumen dengan menggunakan algoritma RSA dan dapat membantu menyelesaikan permasalahan dalam pengamanan data yang terdapat didalam file dokumen tersebut.Kata Kunci: File Dokumen, Kriptografi, Algoritma RSA, Asimetris, Keamanan data