bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Fluorescence detection of three types of pollutants based on fluorescence resonance energy transfer and its comparison with colorimetric detection
Authors: Kong Yifei , Liu Dan , Guo Xinran , Chen Xinyue 
Published: April 2023

Abstract
Three types of pollutants investigated are commonly used and pose a great threat to both environment and human health. This article was aimed at the representative three kinds of pollutants, mainly to explore a fast, simple, sensitive, visible naked eye detection method. The colorimetric detection by gold nanoparticles (AuNPs) was first tried. The cross-linking reaction occurred owing to the strong forces between targets and AuNPs, leading to the aggregation and color change. However, large-scaled aggregation was easily formed and settled which failed to realize accurate quantification. Thus, AuNPs were considered to be used in fluorescence detection as reaction bridge. The introduction of AuNPs could effectively quench the fluorescence of Rhodamine B based on the fluorescence resonance energy transfer (FRET). Since three types of pollutants had great affinity with AuNPs, Rhodamine B dissociated from the solution, causing the recovery of red fluorescence. The fluorescent responses toward three targets were established, resulting in the good linearity in a wide range with low detection limits. The selectivity was well investigated among the same types of interferences. This simple, fast and sensitive fluorescence detection system was first used for simultaneously detecting three types of pollutants and finally successfully applied to real samples.
First evidence of a key role of the peroxymonosulfate radical (SO5•−) in a Fenton-like process based on metabisulfite
Authors: farinelli giulio , Gil Angela Garcia , Marugan Javier , Minella Marco , Fabbri Debora , Laurenti Enzo , Tiraferri Alberto , Vione Davide 
Published: April 2023

Abstract
The complex of iron and tetra-amido macrocyclic ligand (Fe-TAML), or Collin’s reagent, is a promising catalyst to carry out Fenton-like degradation of contaminants. Previously, the ability of Fe-TAML to activate sulfite and metabisulfite (S2O52-, MBS) other than hydrogen peroxide, was reported, which could lead to cheaper, safer, and almost equally efficient oxidation of contaminants. However, the actual species involved in the Fe-TAML/MBS is not yet clear. Experiments reported here included a combination of tests involving the presence of various scavengers, modified atmospheres (air vs. N2), as well as measurements with electron paramagnetic resonance. Specifically, the tests were designed to positively exclude the presence of various active species and identify the most likely one. Thus, we theorize the involvement of the peroxymonosulfate radical (SO5·-) in the presence of the Collin’s reagent and metabisulfite and provide indirect evidence of the activity of this species. Specifically, we demonstrated that this system triggers a Fenton-like process, able to induce the degradation of phenol. The exploitation of such a reactive species may be favourable, as it may selectively induce the degradation of target contaminants in the presence of excess interfering agents, starting from the cheap, safe, and easily handled metabisulfite, which is an interesting substitute for hydrogen peroxide.
Does aggressive treatment make benefit for patients with spinal metastasis in years lost due to disability?
Authors: Su Meng-Lei , Lo Wei-Cheng , Chiang Yung-Hsiao , Lo Wen-Cheng , Lin Jiann-Her 
Published: April 2023

Abstract
Introduction
Spinal metastasis (SM) can cause pain and neurological deficits that devastate the life-quality of patients. While surgery can relieve pain and prevent neurological deficits, its high-costs and the relatively short-expected survival time raises concerns about cost-effectiveness. Therefore, this study aims to investigate whether aggressive treatment can decrease years lost due to disability (YLD) in SM patients and to identify the factors that distinguish cost-effective from non-cost-effective groups. This retrospective study included 41 patients who received aggressive treatment for SM between 2016 and 2021. We developed a worst-case model assuming that patients would only receive palliative care to assess the cost-effectiveness of aggressive treatment. We compared the YLD for each patient in the real-world scenario to those in the worst-case scenario and conducted a cost-effective analysis of medical expenses during hospital admission and one-year postoperative follow-up. The patients were divided into cost-effective and non-cost-effective groups based on cost-effectiveness definition. 13 patients were categorized as cost-effective, while 28 patients were not. The cost-effective group had better preoperative performance status (PS), lower spinal instability neoplastic score, longer predicted survival time based on the Tomita score/revised Tokuhashi score, and higher risk of deterioration based on the image study/SM location.
Thermoelectric coupling effect in BNT-based pyroelectric ceramics for low-grade temperature driven energy harvesting
Authors: Shen Meng , Liu Kun , Zhang Guanghui , Li Qifan , Zhang Qingfeng , Zhang Guangzu , Zhang Haibo , Jiang Shenglin , Chen Yong , Yao Kui 
Published: April 2023

Abstract
Pyroelectric energy harvesting has received increasing attention in recent years due to its ability to convert low-grade waste heat into electricity for sustainable development. However, the low output energy density driven by low-grade temperature limits its practical applications. Here, we report a high-performance hybrid BNT-BZT-xGaN thermal energy harvesting system with environmentally friendly lead-free BNT-BZT pyroelectric matrix and high thermal conductivity GaN as dopant. The theoretical analysis of BNT-BZT and BNT-BZT-xGaN with x = 0.1 wt% suggests that the introduction of GaN facilitates the resonance vibration between Ga and Ti, O atoms, which not only contributes to the enhancement of the lattice heat conduction, but also improves the vibration of TiO6 octahedra, resulting in simultaneous improvement of thermal conductivity and pyroelectric coefficient. Therefore, a thermoelectric coupling enhanced energy harvesting density of 80 µJ cm−3 has been achieved in BNT-BZT-xGaN ceramics with x = 0.1 wt% driven by a temperature gradient of 2 oC, at the optical load resistance of 600 MΩ. This theoretical understanding provides a guidance to establish new principles for designing pyroelectric materials with further improved thermal energy harvesting performance.
Repurposing niclosamide as a novel anti-SARS-Cov-2 drug by restricting entry protein CD147
Authors: Yang Zhe , Zhang Qi , Wu Xiaoqing , Hao Siyuan , Hao Xinbao , Jones Elizabeth , Zhang Yuxia , Qiu Jianming , Xu Liang 
Published: April 2023

Abstract
Background The burst of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing the global COVID-19 pandemic. But until today only limited numbers of drugs are discovered to treat COVID-19 patients. Even worse, the rapid mutations of SARS-CoV-2 compromise the effectiveness of existing vaccines and neutralizing antibodies due to the increased viral transmissibility and immune escape. CD147-spike protein, one of the entries of SRAR-CoV-2 into host cells, has been reported as a promising therapeutic target for developing drugs against COVID-19.Methods CRISPR-Cas9 induced gene knockout, western blotting, tet-off protein overexpression, ribonucleoprotein IP and RNA-IP were used to confirm the regulation of HuR on mRNA of CD147. Regulation of niclosamide on HuR nucleo-translocation was assessed by immunofluorescence staining of cell lines, IHC staining of tissue of mouse model and western blotting. Finally, the suppression of niclosamide on SARS-CoV-2 infection induced CD147 was evaluated by ACE2-expressing A549 cells and western blotting.Results We first discovered a novel regulation mechanism of CD147 via the RNA-binding protein HuR. We found that HuR regulates CD147 post-transcription by directly bound to its 3'-UTR. The loss of HuR reduced CD147 in multiple cell lines. Niclosamide inhibited CD147 function by blocking HuR cytoplasmic translocation and diminishing CD147 glycosylation. SARS-CoV-2 infection induced CD147 in ACE2-expressing A549 cells, which could be neutralized by niclosamide in a dose-dependent manner.Conclusion Together, our study reveals a novel regulation mechanism of CD147 and niclosamide can be repurposed as an effective COVID-19 drug by targeting the virus entry, CD147-spike protein.
Effects of different intensity of atrial pacing on atrial electrophysiology and nerve remodeling
Authors: Yang Zhi , Fan Jianing , Xue Wenjing , Lei Fuhua , Zhang Feng 
Published: April 2023

Abstract
Background and Objective
Atrial fibrillation (AF) could be induced by different intensity of atrial pacing, however the detail information during these processes have not been fully explored. The aim of this study was to evaluate the effects of different intensity of atrial pacing on atrial electrophysiology.
Methods
Twenty-four dogs were randomly subjected to 16 hours atrial pacing at 500 beats/min (bpm) or 1000 bpm as follows: no stimulation (control, n = 8), 500 bpm stimulation (500 bpm, n = 8) and 1000 bpm stimulation (1000 bpm, n = 8). Programmed and burst atrial pacing were performed at baseline and at the end of every 2 hour to determine AF inducibility, sustained time of AF and atrial effective refractory period (ERP). Moreover, the electrical activities of vagus nerve including discharge frequency, signal area were also recorded. In addition, serum acetylcholine (Ach) was determined by ELISA to explore the relationship with AF and vagus nerve features.
Results
Increased AF inducibility, sustained time of AF and ERP were found in dogs from 1000 bpm group compared to 500 bpm group. Moreover, increased discharge frequency and signal area of vagus nerve were also found in dogs from 1000 bpm group compared to 500 bpm group. Correlation was found between the serum Ach and AF.
Conclusions
Our results demonstrated that different intensity of atrial pacing exerted different effects on atrial electrophysiology and nerve remodeling, while high-frequency electrical stimulation are more prone to autonomic nervous activity induction.
Seasonal distribution, source identification, health and eco-toxicological risk assessment of Polycyclic Aromatic Hydrocarbons (PAHs) in water and sediment from the Danube River in Hungary
Authors: Grmasha Ruqayah Ali , Stenger-Kovács Csilla , Al-sareji Osamah J. , Al-Juboori Raed A. , Meiczinger Mónika , Andredaki Manolia , Idowu Ibijoke A. , Hashim Khalid S. 
Published: April 2023

Abstract
The Danube is one of the largest transboundary rivers word-wide, having several tributaries. The discharges from industrial activities and wastewater treatment facilities affect the river's aquatic environment. These discharges pose a serious risk to aquatic life by degrading the water and sediment quality. Therefore, 16 Polycyclic Aromatic Hydrocarbons (PAHs) compounds in six different locations were examined along the river over 12 months to investigate the temporal and special variations of the compounds in water and sediment. The findings highlighted a broad variance range in PAHs concentration in water within a year, ranging from 224.85 ng/L in summer to 365.87 ng/L in winter, whereas PAHs in sediment samples recorded values ranging from 316.72 ng/g in dry weight in summer to 422.98 ng/g in dry weight in winter. The overall results indicate that the putative anthropogenic sources of PAHs were of pyrolytic and pyrogenic origin, with pyrogenic sources being more prominent. Generally, except for Acenaphthylene and Fluorene concentrations, the eco-toxicological concerns for the aquatic environment of the Danube River do not pose a significant threat. In addition, the combined impact of the 16 PAHs pollutants in sediments suggests a low chance for negative biological impacts and low ecological risk. The total ILCR for both children and adults is more than 1/104 in all seasons, with the highest values recorded in spring followed by winter time, which becomes a matter of urgency.
Numerical Simulation of the Effects of Deposits on the Flow Fields and Separation Performance of Cyclones in a Commercial RFCC Reactor
Authors: Zhou Faqi , Dai Chaolei , Guo yanan , Xu Jun , Fu Shuangcheng , Chong peng 
Published: April 2023

AbstractCarbon deposits in Residue Fluidized Catalytic Cracking (RFCC) separators could be a safety hazard, they reduce efficiency, may also cause unscheduled shutdowns in industrial production. In this paper, the effect of coking on flow fields and separation performances was studied with an industrial cyclone separator. The time-averaged and dynamic flow-fields were affected significantly by the coking, as well as separation performance. The tangential and axial velocities decreased due to increased coking, with a maximum decrease of 102.9% and 60.7%, respectively. And the pressure drops decreased due to the vortex dissipation losses, with a maximum decrease of 33.8%. The vorticity also aggravated the breakage with the increasing coking as the breakage position moved up to the cone section. The instability of the flow-field affected the separation efficiency, and the separation efficiency, in turn, as a function of coking types, with a maximum decrease of 18.9%. With increased coking, both the pressure eccentricity and dynamic pressure standard deviation increased. And the main frequency of pressure fluctuation decreased from 37 Hz to 9 Hz. This study has the potential to further the understanding of the flow-field characteristics and performance for different coking types in cyclone separators.
Myeloid PTEN controls STING-induced inflammation and necroptosis through NCID/NRF2 signaling in APAP-induced liver injury
Authors: Yang Tao , Qu Xiaoye , Zhao Jiaying , Wang Xiao , Wang Qian , Dai Jingjing , Zhu Chuanlong , Li Jun , Jiang Longfeng 
Published: April 2023

Abstract
Background
Phosphatase and tensin homolog deleted on chromosome 10(PTEN) signaling has been known to play a critical role in maintaining cellular and tissue homeostasis, which also has an essential role in the inflammatory response. However, it remains unidentified whether and how the myeloid PTEN may govern the innate immune signaling stimulator of interferon genes (STING) mediated inflammation and hepatocyte necroptosis in APAP-induced liver injury (AILI).
Methods
Myeloid-specific PTEN knockout (PTENM−KO) and floxed PTEN (PTENFL/FL) mice were treated with APAP (400 mg/kg) or PBS. In a parallel in vitro study, bone marrow-derived macrophages (BMMs) were isolated from these conditional knockout mice and transfected with CRISPR/Cas9-mediated Notch1 knockout (KO) or CRISPR/Cas9-mediated STING activation vector followed by LPS (100 ng/ml) stimulation.
Results
Here, we report that myeloid-specific PTEN knockout (PTENM−KO) mice were resistant to oxidative stress-induced hepatocellular injury with reduced macrophage/neutrophil accumulation and proinflammatory mediators in AILI. PTENM-KO increased the interaction of nuclear Notch intracellular domain (NICD) and nuclear factor (erythroid-derived 2)-like 2 (NRF2) in the macrophage nucleus, reducing reactive oxygen species (ROS) generation. Mechanistically, it is worth noting that macrophage NICD and NRF2 co-localize within the nucleus under inflammatory conditions. Additionally, Notch1 promotes the interaction of immunoglobulin kappa J region (RBPjκ) with NRF2. Disruption of the Notch1 signal in PTEN deletion macrophages, reduced RBPjκ and NRF2 binding, and activated STING signaling. Moreover, PTENM-KO macrophages with STING activated led to TNF-α release and ROS generation, resulting in hepatocyte necroptosis upon co-culture with primary hepatocytes.
Conclusions
Our findings demonstrate that the myeloid PTEN-NICD/NRF2-STING axis is critical to regulating oxidative stress-induced liver inflammation and necroptosis in AILI and implies the therapeutic potential for managing sterile liver inflammation.
Growth hormone reduces aneuploidy and improves oocytes quality by JAK2-ERK1/2 pathway in aging mice
Authors: Luo Yunyao , Zeng Xi , Zhu Ling , Li Chong , Xie Juan , Dong Qiang , Sun Qingyuan , Huang Guoning , Li Jingyu 
Published: April 2023

Abstract
Background The global delay in women’s reproductive age has raised concerns about age-related infertility. The decline in oocyte quality is a limiting factor of female fertility; however, strategies to maintain the oocyte quality of aging women are not available. Here, we investigated the effects of growth hormone (GH) supplementation on aneuploidy of aging oocytes.Methods For the in vivo experiments, the aging mice (8-month-old) were intraperitoneally injected with GH every day for 8 weeks. For the in vitro experiments, germinal vesicle oocytes from aging mice were treated with GH. The impacts of GH on ovarian reserve before superovulation was evaluated. Oocytes were retrieved to determine oocyte quality, aneuploidy and developmental potential parameters. Quantitative proteomics analysis was applied to investigate the potential targets of GH in aging oocytes.Results In this study, we showed that GH supplementation in vivo not only alleviated the decline in oocyte number caused by aging, but also improved the quality and developmental potential of aging oocytes. Strikingly, we found that GH supplementation reduced aneuploidy in aging oocytes. Mechanically, in addition to improving mitochondrial function, our proteomic analysis indicated that the ERK1/2 pathway might be involved in the reduction in aneuploidy of aging oocytes, as confirmed both in vivo and in vitro. In addition, JAK2 might mediate the regulation of ERK1/2 by GH.Conclusions In summary, our findings reveal that GH supplementation protects oocytes from aging-related aneuploidy and enhances the quality of aging oocytes, which has clinical implications in assisted reproduction of aging women.
Pre-pubertal sublingual immunotherapy is more effective than immunotherapy during puberty in allergic rhinitis and asthma
Authors: Zhu Yongjun , Cheng Nan , Xiao Yun , Yan Lin , Fan Dachuan , Cao Wei , Yang Jianming 
Published: April 2023

Abstract

Objective: To evaluate the clinical efficacy of sublingual-specific immunotherapy (SLIT) and pulmonary function in children with allergic rhinitis and asthma before and after puberty.
Methods: This retrospective analysis included 136 patients aged 4-18 years with allergic asthma and rhinitis who received two years of SLIT treatment. Patients were divided into two groups based on age: the prepubertal group (4-10 years old) and the pubertal group (11-18 years old). After half a year, one year, and two years of SLIT, the total nasal symptom score (TNSS), total rhinitis medication score (TRMS), daytime asthma symptom score (DASS), nighttime asthma symptom score (NASS), total asthma medication score (TAMS), asthma control test (ACT), and peak expiratory flow rate (PEF%) were evaluated and compared with the baseline before treatment.
Results: In both groups, TNSS, TRMS, DASS, NASS, TAMS, ACT, and PEF% improved significantly after half a year, one year, and two years of SLIT treatment. After half a year of treatment, prepubertal patients showed better therapy for TNSS, DASS, NASS, and TAMS compared to the pubertal group. The TAMS of the pubertal group was higher than that of the prepubertal group after one year of treatment. Finally, the PEF% showed better therapy compared to the pubertal group.
Conclusion: SLIT treatment with Dermatophagoides farinae drops can effectively control the symptoms of rhinitis and asthma in children with allergic rhinitis and asthma before and after puberty, reduce the use of symptomatic drugs, significantly improve the pulmonary function of patients, and have better effects on asthma in prepubertal children than in adolescents.
Impact of General Anesthesia on Ablation Catheter Stability During Pulmonary Vein Isolation: An Assessment based on a Novel Measurement Approach
Authors: Kuno Shimpei , Nakano Yusuke , Suzuki Yasushi , Ando Hirohiko , Suzuki Wataru , Takahashi Hiroshi , Amano Tetsuya 
Published: April 2023

Abstract
Catheter ablation for atrial fibrillation (AF), during pulmonary vein isolation (PVI), is performed under general anesthesia (GA) or conscious sedation (CS). GA during PVI may improve treatment outcomes, via improving catheter stability. However, the improvement in catheter stability with GA compared to that with CS is unclear. Therefore, we aimed to verify the influence of GA on ablation catheter stability during PVI compared with that of CS by directly assessing catheter movement. Patients who underwent initial ablation using the EnSite Precision™ mapping system were recruited. The patients were divided into two groups (GA group or CS group), and the ablation catheter stability during PVI by the distance traveled by the catheter distal tip per second, clinical periprocedural characteristics, and periprocedural complications was compared. Among 69 consecutive patients, data from 30 patients (17 patients in the GA group and 13 patients in the CS group) and the distance traveled per second on 148,976 points/patient were evaluated. The GA group had a significantly smaller catheter tip travel distance than the CS group (0.92 (0.82‒1.16 vs. 1.25 (1.14‒1.38), p = 0.01). Therefore, GA during PVI for AF provides greater catheter stability than CS, which may contribute to more accessible and safer PVI procedures.
Identification of the key roles of different hexokinases on the diagnosis, prognosis, tumor immunity, drug response: evidence from pan-cancer analysis and construction prognostic HKs signatures
Authors: Shang Zebin , Qiu Wanting , Wang Guanghui , Zhang Qiang 
Published: April 2023

Abstract
Background Metabolic reprogramming is a key feature of cancer cells and is considered a new hallmark of cancer. With our increasing understanding and appreciation of tumor biology and metabolic complexity, targeting or regulating the expression of certain key metabolic enzymes of metabolic reprogramming may be important strategies for tumor therapy. Glycolysis is an essential part of the complex network of glucose metabolism, and hexokinases (HKs) are the key factors of the glycolysis pathway. Although HKs have also received attention in tumors, their roles in tumors are still not fully and systematically explored, particularly in immunization.Methods Through using multiple online datasets, including the TCGA database, Genecards database, CellMiner database, and Deepscreening database, and combining multiple algorithms, the association of HKs with prognosis, Tumor microenvironment (TME), Tumor immunity, and drug sensitivity were investigated. HKs were also evaluated for their prognostic relevance to specific tumor types and their synergistic effects by constructing prognostic HKs signatures. In particular, the deep learning algorithm predicted the active molecules binding to HKs.Results Cox and survival analysis suggested that HKs were significant factors influencing tumor progression. HKs expression levels strongly correlated with TME, RNAss, and Tumor immunity. Their influences varied in diverse tumors or some specific tumor types. In addition, the relationship between gene expression of HKs and drug sensitivity was investigated, and the results suggested the potential of targeting HKs, especially HKDC1 to improve drug resistance. Furthermore, a validation screen of drug prediction and molecular docking obtained several molecules targeting HKs. Finally, the roles of HKs were confirmed in Brain Lower Grade Glioma (LGG) and Acute Myeloid Leukemia (LAML) by constructing the HKs signatures, further, their homogeneity and heterogeneity were elaborated.Conclusion Our systematic study revealed the significant roles of HKs in tumorigenesis and metastasis, as well as their impact and diverse correlations on tumor immune and metabolic activity. The clinical application of HKs is a viable target and offers the new clinical prospects, especially in the development of personalized medicines for the treatment of LGG and LAML.