bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular docking and experimental validation of the effect of ergothioneine on heat shock protein-70 following endurance exercise by Arabian stallions
Authors: Adah Adakole Sylvanus , Ayo Joseph Olusegun , Adah Deborah Arimie , Nwonuma Charles Obiora , Lawal Teslim Alabi 
Published: January 2023

Abstract
Background
Exercise-induced oxidative stress is a challenge in equine sports. This study aims at determining the effects of ergothioneine on heat shock protein-70 (HSP-70) following the stress of an endurance exercise of 30 km by Arabian stallions. Molecular docking was also done to investigate the interaction between the ligand ergothioneine and heat shock protein-70 using sulfogalactosylceramide and sulfogalactoglycerolipid as standards.
The study involved a total of 18 clinically healthy stallions, with an average age of 6.7 ± 2.4 years and an average weight of 411.54 ±12.46 kg. Only clinically healthy stallions were selected as subjects. The stallions were divided into two groups of nine stallions each. Group I (ERGX) was administered ergothioneine at a dose of 0.02 mg/kg once daily orally for four weeks while group II (ERGN) was not administered ergothioneine. The activities of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase were determined in the two groups before and post-exercise. The concentrations of malondialdehyde and HSP-70 were also determined.
Results
The results obtained showed that the activities of the antioxidant enzymes and concentration of HSP-70 were higher (P < 0.05) in the ERGX group compared to the ERGN group. The concentration of malondialdehyde was however lower in the ERGX group. Following molecular docking, ergothioneine and the selected standards have common amino acids at the site of interaction with the target protein (HSP-70) suggesting that ergothioneine may have a modulatory effect on the synthesis of HSP-70.
Conclusion
The results obtained indicated that ergothioneine modulated the synthesis of HSP-70 and the biomarkers of oxidative stress. It was therefore concluded that ergothioneine may be beneficial to horses subjected to endurance exercise.
A Systematic Literature Review: Choice Bracketing in Decision making
Authors: Qandeel Mahmoud Salameh 
Published: January 2023

Abstract
Objectives

In this research, the author revised the most controversial cited articles, which have 100 citations or more, between 1994 and 2022 to investigate the main reasons behind narrow bracketing and understand if it is rational psychological behavior or biased. Knowing the result would give scholars room to tackle this issue and look for more enhancements.

Methods

All open-access and non-restricted articles through the University of Pécs website were included, as were downloadable handbooks as well, but books were excluded. Due to time limitations and grasping pure results, the articles with 100 citations and above between 1994 and 2022 were selected.

Results

The author discovered that scholars discussed "choice bracketing" from their niche corner of science and letting other factors influence their research after thoroughly researching the literature. Accepting or rejecting the lottery, as well as decision differences between two separate choices, or a binary of choices, are not solely determined by determining factors such as bracketing or motivation, and they are not necessarily biased.

Conclusions

The author has elaborated and drawn maps for most factors that intervene in decision-making. However, by collecting the most debatable opinions from the literature, it was found that people’s decisions are driven by maximizing utility and loss aversion regarding wealth and income, individual psychology (emotions, heuristics, mental accounting), limitations of rationality and uncertainty, preferences and motivation, diversification limits, and time allocation. However, this is a psychological mode of thought that is limited to stimuli, timing, and rule factors.
JEL Code: A14, D1, D71, D81, D83.
In silico analysis of NHP2 membrane protein, a novel vaccine candidate present in the RD7 region of Mycobacterium tuberculosis
Authors: Kootery Kaviya Parambath , Sarojini Suma 
Published: January 2023

Abstract
Mycobacterium tuberculosis, the etiological agent of tuberculosis, is one of the most tricky pathogens. We have only a few protective shields, like the BCG vaccine against the pathogen, which itself has poor efficacy in preventing adult tuberculosis. Even though different vaccine trials for an alternative vaccine have been conducted, those studies have not shown much promising results. In the current study, advanced computational technology was used to study the potential of a novel hypothetical mycobacterial protein, identified by subtractive hybridization, to be a vaccine candidate. NHP2 (Novel Hypothetical Protein 2), housed in the RD7 region of the clinical strains of M. tuberculosis, was studied for its physical, chemical, immunological and structural properties using different computational tools. PFAM studies and Gene ontology studies depicted NHP2 protein to be functionally active with a possible antibiotic binding domain too. Different computational tools used to assess the toxicity, allergenicity and antigenicity of the protein indicated its antigenic nature. Immune Epitope Database (IEDB) tools were used to study the T and B cell determinants of the protein. The 3D structure of the protein was designed, refined and authenticated using bioinformatics tools. The validated tertiary structure of the protein was docked against the TLR3 immune receptor to study the binding affinity and docking scores. Molecular dynamic simulation of the protein-protein complex formed were studied. NHP2 was found to activate host immune response against tubercle bacillus and could be explored as a potential vaccine in the fight against tuberculosis.
Case Report: A case of primary intracranial parasagittal meningeal angiosarcoma
Authors: Cao Jun , Fang Jincheng , Jiang Xiaochun , Di Guangfu , Shen Jun 
Published: January 2023

Abstract
Background
Angiosarcoma, also known as malignant hemangioendothelioma, is a rare vasogenic malignant tumor, commonly found on the skin of the head and neck, rarely occurring in the intracranial region.As for intracranial meningeal angiosarcoma, only 7 cases have been reported and there is no clinical study with large sample size. We report here a case of parasagittal meningeal angiosarcoma.
Case Description:
A 48-year-old Chinese male patient was admitted to our hospital due to headache accompanied by bilateral lower limb weakness. On admission, CT showed a high-density mass on both sides of the sagittal sinus at the top of the frontal lobe. We performed exploratory surgical resection of the tumor. During the operation, it was found that the tumor originated from the dura mater and extensively invaded the surrounding brain tissue and skull, and the surrounding hemosiderin deposition was observed. Postoperative pathology suggested angiosarcoma.
Conclusions
Intracranial meningeal angiosarcoma is difficult to accurately diagnose before surgery, so radiologists and neurosurgeons need to strengthen their understanding of this disease. The presence of extensive superficial hemosiderin deposition during operation may contribute to the diagnosis, and immunohistochemistry is very important for the diagnosis of intracranial angiosarcoma.
Research on the Content of Plumbago auriculata (L.) Hairy Roots Mediated by Agrobacterium Rhizogenes and Its Plumbagin
Authors: ZHAO Zian , Gao Suping , Hu Ju , Lei Ting , Zhou Yunzhu , Li Yirui , Liu Cailei 
Published: January 2023

Abstract
Agrobacterium rhizogene-mediated genetic transformation of hairy roots is an effective method to obtain secondary metabolites. In accordance with different genotypes, it is very specific and difficult to set up a stable genetic transformation system. The plumbagin is found in the roots of Plumbago auriculata L., a secondary metabolite with significant medicinal value, but the common root grows slowly, its accumulation period is lengthy (2–6 years). In this paper, we first explored the most effective A. rhizogene-mediated (A4, ATCC 15834, and LBA 9402) genetic transformation to induce hairy root of P. auriculata, and evaluated the plumbagin concentration in different root. The results showed that the leaves were soaked with bacterial solution for 25–30 min and then transferred to 1/2 MS + AS 100 µmol·L− 1 solid medium without preculture for 2-5d. After co culture, the leaves were transferred to 1/2 MS + Cef and sterilized with cefotaxime sodium. Under this scheme, all strains can induce hairy roots, with ATCC 15834 having the highest hairy root induction rate (86.78 ± 0.74%) and the earliest root emergence time (8.33 ± 0.58 d). 1-month-grown hairy root showed an increase in plumbagin content compared with the root of the same age group and 1-year-old live seedlings, with PAHR 15834 having the highest content of 38.95 mg·g− 1 DW, which was 72.13 times higher than the same age group and 3.95 times higher than that of 1-year-old live seedlings. This is an important experimental basis for further investigation of the biosynthesis mechanism of plumbagin and the feasibility of subsequent commercial production.
PROphylaxis for paTiEnts at risk of COVID-19 infecTion (PROTECT-V)
Authors: Humphrey Toby JL , Dosanjh Davinder , Hiemstra Thomas , Richter Alex , Chen-Xu Michael , Qian Wendi , Jha Vivekanand , Gatley Katrina , Adhikari Rakshya , Dowling Francis , Smith Rona Marie 
Published: January 2023

Abstract
Background: Despite the introduction of vaccination, there remains a need for pre-exposure prophylactic agents against SARS-CoV-2. Several patient groups are more vulnerable to SARS-CoV-2 infection by virtue of underlying health conditions, treatments received or suboptimal responses to vaccination.
Methods: PROTECT-V is a platform trial testing pre-exposure prophylactic interventions against SARS- CoV-2 infection in vulnerable patient populations (organ transplant recipients; individuals with oncological/haematological diagnoses, immune deficiency, or autoimmune diseases requiring immunosuppression or on dialysis). Multiple agents can be evaluated across multiple vulnerable populations sharing placebo groups, with the option of adding additional treatments at later time points as these become available. The primary endpoint is symptomatic SARS-CoV-2 infection, and each agent will be independently evaluated in real-time when the required number of events occur. Presently, three agents are approved in the platform: intranasal niclosamide, nasal and inhaled ciclesonide and intravenous sotrovimab.
Discussion: Despite the introduction of vaccination, there remains a need for pre-exposure prophylactic agents against SARS-CoV-2. Several patient groups are more vulnerable to COVID-19 disease by virtue of underlying health conditions, treatments received or suboptimal responses to vaccination.
Trial registration: Clinicaltrials.gov: NCT04870333; EudraCT: 2020-004144-28.
Phosphate Fertilizers Broadcast and Applied on Rows in a High Fertility Tropical Soil Cultivated with Two Soybean Seasons
Authors: Barbosa Kássia de Paula , Rodrigues Carlos Ribeiro , Costa Ándreia Mendes da , Couto Raphael Lopes do , Cantão Veridiana Cardozo Gonçalvez , de Menezes Carlos César Evangelista , Rodrigues Tatiana Michlovská , Abreu Tâmara Pontes , Trindade Polyanna Ribeiro , Castoldi Gustavo , Carlos Leandro 
Published: January 2023

Abstract

Introduction
The efficiency of phosphate fertilization can be affected by phosphorus sources, application forms, soil characteristics, and plant species.
Aims
The aim of this study was to evaluate the responses of soybean crop under different sources of phosphate fertilizers and their forms of application.
Methods
The experimental design was in randomized blocks in a 4 x 2 + 1 factorial, being four sources of phosphorus (simple superphosphate, triple superphosphate, monoammonium phosphate and reactive phosphate), two application techniques (broadcasting and on rows) and the control without phosphate fertilization. The variables evaluated were plant height, weight of 1,000 grains, grain and crop residue yield, agronomic efficiency, phosphorus content in leaves at stage R1, grains and crop residues, and P available in soil.
Results
In the 2016/2017 season there was drought stress in vegetative development stage of the plants, and under this condition fertilization with simple and triple superphosphate provided the highest grain yield and phosphorus exportation. In the 2017/2018 crop season, with the lowest water deficit, phosphate fertilization with soluble sources monoammonium phosphate, triple superphosphate and simple superphosphate obtained the highest grain and export yields. The application technique of fertilizers did not alter the efficiency of the sources in both studied seasons. The application of reactive phosphate did not differ from treatments without phosphate fertilization.
Conclusions
Under the conditions of this study, phosphate fertilization increased soybean yield and reduce balance of phosphorus, while the forms of fertilizer application did not influence. The use of simple and triple superphosphate provided better results.
Cleaner energy, higher productivity? China's energy consumption transition and total factor productivity
Authors: Zhang Zhiyuan , Ma Yongfan 
Published: January 2023

Abstract
New energy strategies are crucial to address energy and environmental issues, but the energy consumption transition may also affect firm behavior with unintended economic consequences. Using China's New Energy Demonstration City (NEDC) policy as a shock, this paper constructs a differences-in-differences model and examines the impact of energy consumption transition on firms' total factor productivity (TFP). It is found that the NEDC reduces firms' TFP by about 6.4%. This conclusion still holds after a series of robustness and endogeneity tests. The channel analysis demonstrates that the NEDC changes firms' investment preferences, making them more inclined to financial investments and deviating from their main business, resulting in efficiency losses. Furthermore, we explore that the preference for financial investment is mainly motivated by precautionary motives. This paper shows that the promotion and application of new energy may have certain economic costs. To better balance the benefits and costs of new energy strategies, the government and other relevant departments should increase policy flexibility and perfection.
Seafloor hydrothermal control on ocean dynamics in Enceladus
Authors: Bouffard Mathieu , Choblet Gaël , Amit Hagay , Tobie Gabriel , Cadek Ondrej , Terra-Nova Filipe 
Published: January 2023

Abstract
Cassini observations imply a global ocean underneath Enceladus' ice shell, with hydrothermal seafloor activity. Previous numerical simulations showed that convection in Enceladus' unconsolidated core produces heterogeneous seafloor heat flux explaining the South Pole ice thinning and plume activity. While there is evidence for efficient hydrothermal transport from the seafloor to the plumes, the ocean dynamics is strongly debated. Here, we perform 3-D numerical simulations of the ocean with a very heterogeneous bottom boundary condition from 3-D hydrothermal core simulations. We show that a strong zonal flow inhibits low-latitudes heat transfer while it remains efficient in polar regions, which explains the ice shell variations derived from gravity and topography observations. Using passive tracers, we predict rising times of hours to weeks, compatible with previous predictions. Our simulations confirm that a strong heterogeneous seafloor heat flux concentrates upwellings at the South Pole, efficiently transporting potential bio-signatures from hydrothermal vents to erupting plumes.
Water Quality, Sanitation, Hygiene, and Diarrheal Diseases among Children in Adadle District, Somali Region, Eastern Ethiopia
Authors: Muhummed Abdifatah Muktar , Osman Yahya , Abdillahi Rahma , Hattendorf Jan , Zinsstag Jakob , Tschopp Rea , Cissé Guéladio 
Published: January 2023

Abstract
Even though simple and effective treatments are available for diarrhea, it remains one of the leading causes of death in children under the age of five, accounting for 1.6 million deaths in 2017. Inadequate access to safe drinking water, particularly for pastoralists in Ethiopia, who are compelled to search for water during dry season. Thus, this study aimed to evaluate the quality of drinking water, sanitation, hygiene, and the prevalence of diarrhea among pastoralists’ children in Ethiopian. Methodology: Using a questionnaire, a cross-sectional study of 538 randomly selected households was done in 2018 to assess the prevalence of diarrhea in children younger than five years old and its association with water quality, sanitation, and hygiene. Portable DelAgua field kits were utilized to evaluate sources and home water for Escherichia coli contamination (E. coli). Logistic regression was used to assess the risk factors of water quality and diarrhea. Results: The prevalence of diarrhea was found to be 26.6% and 31.4% in the first and second weeks, respectively. There was an association between the prevalence of diarrhea in children and the consumption of low-quality water, improper storage conditions, caregivers who did not wash their hands prior to feeding a child, whenever their hands were filthy, and children aged 12 to 24 months. In terms of drinking water, households dependent on river water and unprotected dug wells had a considerably greater likelihood of faecal contamination than those dependent on protected water (Barkad). With regard to sanitation, almost all of the households surveyed lacked basic sanitation (95.9%) and hygiene. Conclusion: Water quality, hand-washing practice, water storage conditions, and the age of the children were found to be significantly associated with diarrhea. Thus, the current burden of diarrheal diseases in these children can be reduced by promoting widespread use of proven preventative measures, such as increasing awareness on handwashing, sanitation, waste disposal management and better treatment of stored water, and periodic monitoring of water quality.
Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis.
Authors: Liu Bo-Wen , Song Angxi , Gui Pengkun , Wang Jin , Pan Yao-Jie , Li Chao , Li Shuai , Zhang Yi , Jiang Tao , Xu Yi-Xin , Huo Fu-Chun , Pei Dong-Sheng , Song Jun 
Published: January 2023

Abstract
Long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis and tumor metastasis. However, the underlying mechanisms of lncRNAs in colorectal cancer (CRC) need further exploration. By using data from The Cancer Genome Atlas (TCGA) and GEO databases, we identified a novel CRC-related lncRNA, LINC01594, that is significantly upregulated in CRC and associated with poor prognosis. In vitro and in vivo, gain- and loss-of-function experiments demonstrated that LINC01594 promotes metastasis in CRC. LINC01594 functions as a DNMT1 scaffold, increasing the level of CELF6 promoter methylation. LINC01594 also competitively binds the transcription factor p53, decreasing CELF6 expression. This inhibited the exon skipping of CD44 V4-V7 induced by CELF6. In summary, this study highlights a novel CRC biomarker and therapeutic target, LINC01594, and the findings suggest that the LINC01594-CELF6-CD44 axis might serve as a biomarker and therapeutic target in CRC.
Autophosphorylation of αCaMKII regulates alcohol consumption by controlling sedative effects of alcohol and alcohol-induced loss of excitatory synapses
Authors: Cały Anna , Ziolkowska Magdalena , Pagano Roberto , Salamian Ahmad , Sliwinska Malgorzata , Sotoudeh Narges , Bernaś Tytus , Radwańska Kasia 
Published: January 2023

Abstract
Calcium/calmodulin-dependent kinase II (CaMKII) is a key enzyme at the glutamatergic synapses. CAMK2A gene variants have been linked with alcohol use disorder (AUD) by an unknown mechanism. Here, we looked for the link between αCaMKII autophosphorylation and the AUD etiology. Autophosphorylation-deficient heterozygous αCaMKII mutant mice (T286A+/-) were trained in the IntelliCages to test the role of αCaMKII activity in AUD-related behaviors. The glutamatergic synapses morphology in CeA was studied in the animals drinking alcohol using 3D electron microscopy. We found that T286A+/- mutants consumed less alcohol and were more sensitive to sedating effects of alcohol, as compared to wild-type littermates (WT). After voluntary alcohol drinking T286A+/- mice had less excitatory synapses in the CeA, as compared to alcohol-naive animals. This change correlated with alcohol consumption, was not reversed after alcohol withdrawal and not observed in WT mice. Our study suggests that αCaMKII autophosphorylation affects alcohol consumption by controlling sedative effects of alcohol and preventing synaptic loss in the individuals drinking alcohol. This finding advances our understanding of the molecular processes that regulate alcohol misuse.
Label-free nanoscale mapping of intracellular organelle chemistry
Authors: Greaves George , Kiryushko Darya , Auner Holger , Porter Alexandra , Phillips Chris 
Published: January 2023

Abstract
The ability to image cell chemistry at the nanoscale is key for understanding cell biology, but many optical microscopies are restricted by the ~250nm diffraction limit. Electron microscopy and super-resolution fluorescence techniques beat this limit, but rely on staining and specialised labelling to generate image contrast. It is challenging, therefore, to obtain information about the functional chemistry of intracellular components.
Here we demonstrate a technique for intracellular label-free chemical mapping with nanoscale (~30nm) resolution. We use a probe-based optical microscope illuminated with a mid-infrared laser whose wavelengths excite vibrational modes of functional groups occurring within biological molecules. As a demonstration, we chemically map intracellular structures in human multiple myeloma cells and compare the morphologies with electron micrographs of the same cell line. We also demonstrate quantitative and label-free mapping at multiple wavelengths chosen to target the chemical signatures of macromolecules, including proteins and nucleic acids, in a way that can be used to identify biochemical markers in the study of disease and pharmacology.