bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of SCC Concrete with Additional Materials of Rice Husk Ash
Authors: Amalia  , Tiyani L , Setiawan Y , Hasan M F R 
Published: December 2022

Abstract
Concrete with high ductility, workable, high strength, easy to flow without compaction, and durable is a concrete need in the future. Concrete with this quality, one of which is to make self-compacting concrete (SCC). This paper reports performance of self compacting concrete (SCC) containing rice hush ash. Rice husk ash is waste from burning rice husks. The purpose of this study was to determine the performance of SCC concrete with the addition of rice husk ash. SCC specimens were made using rice husk ash (RHA) and SCC without rice husk ash (NRHA). The specimens were made with water cement ratio 0.30. Superplastisizer used is a type Naptha 511P. The result indicated that the workability of SCC containing rice hush ash (RHA) more workable compare SCC without rice hush ash (NRHA). The initial setting time of SCC with rice hush ash more slowly compare SCC without rice hush ash. The compressive strength, flexural strength, and tensile strength of SCC RHA mor higher compare SCC without RHA (NRHA). The tensile strength value of RHA and without RHA concrete meets the tensile strength requirements of RSNI T-12-2004.
Aceh traditional village landscape patterns as a way of adapting to the climate in modern housing
Authors: Nursaniah C , Qadri L , Fuady Z 
Published: December 2022

Abstract
There are always certain considerations behind the presentation of architectural works by our ancestors, including the formation of traditional Acehnese village landscape patterns. The form of traditional architectural works reflects solutions to the environment, socio-culture, and technology of the supporting community as a form of local wisdom. This paper aims to examine the reasons for the formation of traditional Acehnese village landscape patterns in the past, and how they relate to modern housing landscape patterns in Banda Aceh City based on climate variables. A literature study was conducted to find out the elements and patterns of the village landscape that will be used as parameters to analyze samples of modern housing from three different areas in Banda Aceh City, namely the North, Central, and South. This paper concludes that the pattern of traditional Aceh settlements is formed from housing zoning, public facilities, arable land, and forests as village boundaries with a group pattern. This pattern is formed based on a geographical and climatic approach that is adapted to the contours of the land and building technology, resulting in a mitigation system against frequent storms and a cross-ventilation system for residential buildings. While the relevance of its application to the landscape pattern of modern housing produces different characteristics based on several adjustments to changes in socio-cultural, material, and transformed building technology.
Lands Cover Dynamics of Siais Lake Catchment Area, Tapanuli Selatan District, Indonesia
Authors: Samsuri  , Zaitunah A , Ahmad AG , Pasaribu I 
Published: December 2022

AbstractThe Siais Lake Catchment Area (SLCA) is a water resource that includes various types of land cover and land use in Rianiate village, Angkola Sangkunur sub-district, and Tapanuli Selatan district. The SLCA lake catchment area has altered the land cover and increased the surface area of Siais Lake. The purpose of this study was to determine the type of land cover that changed in the Siais lake catchment area between 1990 and 2018. The land cover classification was obtained using Landsat 5 and Landsat 8 OLI images. Make use of supervised classification. Forest, plantation, bare land, paddy field, settlement, waterbody, shrub, and swamp were the land covers identified in the Lake Siais catchment area. Plantations had the highest percentage of land cover increase from 1990 to 2018, with an increase of 3,995.87%, while paddy fields had the highest decrease, with a decrease of 93.77%. .
Evaluation of Regional Myocardial Work Indices in Pediatric Essential and Renal or Renovascular Hypertension
Authors: Jacquemyn Xander , Van den Eynde Jef , Zhan Junzhen , Doshi Ashish N , Long Rita , Danford David A , Barnes Benjamin T , Kutty Shelby 
Published: December 2022

AbstractBACKGROUNDMyocardial work (MW) is an index of LV function based on pressure–strain loops and brachial cuff pressure measurement. MW has been proposed as more sensitive than conventional functional parameters, as it accounts for afterload and myocardial deformation. However, many studies have been limited to assessment of global MW indices, neglecting regional differences in cardiac associated with hypertension and consequent cardiac remodeling. We aimed to quantify regional MW in pediatric hypertension and compare the findings in renal or renovascular hypertension (RHTN) with essential hypertension (EHTN).METHODSWe retrospectively assessed conventional markers of LV function, and both global and regional MW indices in 78 patients (49 males, 15.4 ± 2.94 years) with EHTN and RHTN.RESULTSPeak systolic strain (PSS) in the basal septal segment was significantly impaired in patients with RHTN compared to EHTN (−13.00% [−15.50%; −13.00%] vs. −15.00% [−17.50%; −13.50%], P = 0.034). Similarly, basal septal MW indices were significantly elevated in patients with EHTN compared to RHTN, including MW efficiency (MWE) (95.0% [93.0%; 98.0%] vs. 94.0% [89.0%; 95.0%], P = 0.004) and constructive work (CW) (1700 mm Hg% (409 mm Hg%) vs. 1520 mm Hg% (336 mm Hg%), P = 0.037). Wasted work (WW) was significantly elevated in the RHTN group (79.0 mm Hg% [28.5 mm Hg%; 104 mm Hg%] vs. 105 mm Hg% [62.0 mm Hg%; 164 mm Hg%], P = 0.010).CONCLUSIONSignificant differences in basal septal PSS and MW indices were observed between EHTN and RHTN. These findings highlight the usefulness of regional MW indices in assessing disease and may help differentiate between etiologies of pediatric hypertension.
Electroanatomical mapping–guided left bundle branch area pacing in patients with structural heart disease and advanced conduction abnormalities
Authors: Richter Sergio , Gebauer Roman , Ebert Micaela , Moscoso Ludueña Cathleen , Scheller Dominik , Lucas Johannes , König Sebastian , Paetsch Ingo , Hindricks Gerhard , Döring Michael 
Published: December 2022

AbstractAimsLeft bundle branch area pacing (LBBAP) can be technically challenging and fluoroscopy-intense. Three-dimensional electroanatomical mapping (EAM) facilitates non-fluoroscopic lead navigation and electrogram mapping. We sought to prospectively evaluate the feasibility, safety, and outcomes of routine EAM-guided LBBAP in patients with structural heart disease (SHD) and advanced conduction abnormalities.Methods and resultsConsecutive patients with SHD and conduction abnormalities who underwent an attempt at EAM-guided LBBAP were included. The feasibility, safety, procedural, and mid-term outcomes were evaluated. Electrical, echocardiographic, and clinical parameters were assessed at implantation and last follow-up. Thirty-two patients (68 ± 18 years; 19% female) were included, of which 75% had intrinsic QRS &gt; 150 ms, 53% left bundle branch block, and 25% right bundle branch block. Primary EAM-guided LBBAP was successful in 29 patients (91%). The procedural duration was 95 (70–110) min, total fluoroscopy time 0.93 (0.40–1.73) min, and total fluoroscopy dose 35.4 (20.5–77.2) cGy cm2. Paced QRS duration (QRSd) was significantly shorter than intrinsic QRSd (121.9 ± 10.7 vs. 159.2 ± 34.4 ms; P &lt; 0.001) and remained stable during the mean follow-up of 7.0 ± 5.9 months. The LBBAP capture threshold was 0.57 ± 0.23 V/0.4 ms at implantation and remained low during follow-up (0.58 ± 0.18 V/0.5 ± 0.2 ms; P = 0.877). Overall left ventricular ejection fraction improved significantly from 44.2 ± 14.3% at baseline to 49.4 ± 13.1% at follow-up (P = 0.009), New York Heart Association class from 2.4 ± 0.6 to 1.8 ± 0.6 (P = 0.002), respectively. No complications occurred that required intervention.ConclusionRoutine near-zero fluoroscopy EAM-guided LBBAP can safely be performed in patients with SHD and advanced conduction abnormalities with high success rates and favourable mid-term outcomes. Further studies are needed to investigate whether the use of EAM improves the overall outcome of conduction system pacing and to identify specific patient populations who benefit the most from EAM-guided lead implantation.
The expression effects of uncivil disagreement: the mechanisms of cognitive dissonance and self-perception
Authors: Liang Hai , Ng Yee Man Margaret 
Published: December 2022

Abstract
Political incivility is pervasive and still on the rise. Although empirical studies have examined the effects of exposure to political incivility in different contexts, few have attempted to investigate the expression effects of incivility on its senders. This study proposes two mechanisms—cognitive dissonance and self-perception—to explain the expression effects of political incivility on anger, perceptions of incivility, and political participation. The study conducts a population-based online survey experiment (N = 413) in Hong Kong. Participants were either forced to express uncivil or civil disagreements or did so voluntarily. The results suggest that expressing uncivil disagreement increases anger and perceptions of incivility. However, no difference is found between the forced and self-selection conditions, indicating that self-perception is more applicable than cognitive dissonance. In addition, the study finds that expressing uncivil disagreement influences political participation via both anger and perceptions of incivility, though the effects run in opposite directions.
Vertical integration of oncologists and cancer outcomes and costs in metastatic castration-resistant prostate cancer
Authors: Hu Xin , Lipscomb Joseph , Jiang Changchuan , Graetz Ilana 
Published: December 2022

AbstractBackgroundThe share of oncology practices owned by hospitals (ie, vertically integrated) nearly doubled from 2007 to 2017. We examined how integration between hospitals and oncologists affected care quality, outcomes, and spending among metastatic castration-resistant prostate cancer (mCRPC) patients.MethodsUsing Surveillance, Epidemiology, and End Results–Medicare linked data and the Medicare Data on Provider Practice and Specialty, we identified Medicare beneficiaries who initiated systemic therapy for mCRPC between 2008 and 2017 (n = 9172). Primary outcomes included 1) bone-modifying agents (BMA) use, 2) time on systemic therapy, 3) survival, and 4) Medicare spending for the first 3 months following therapy initiation. We used a differences-in-differences approach to estimate the impact of vertical integration on outcomes, adjusting for patient and provider characteristics.ResultsThe proportion of patients treated by integrated oncologists increased from 28% to 55% from 2008 to 2017. Vertical integration was associated with an 11.7 percentage point (95% confidence interval [CI] = 4.2 to 19.1) increased likelihood of BMA use. There were no satistically significant changes in time on systemic therapy, survival, or total per-patient Medicare spending. Further decomposition showed an increase in outpatient payment ($5190, 95% CI = $1451 to $8930) and decrease in professional service payment (−$4757, 95% CI = −$7644 to −$1870) but no statistically significant changes for other service types (eg, inpatient and prescription drugs).ConclusionsVertical integration was associated with statistically significant increased BMA use but not with other cancer outcomes among mCRPC patients. For oncologists who switched service billing from physician offices to outpatient departments, there was no statistically significant change in overall Medicare spending in the first 3 months of therapy initiation. Future studies should extend the investigation to other cancer types and patient outcomes.
Circular RNA in autoimmune diseases: special emphasis on regulation mechanism in RA and SLE
Authors: Huang Yurong , Xue Qiuyun , Cheng Chenglong , Wang Yuting , Wang Xiao , Chang Jun , Miao Chenggui 
Published: December 2022

Abstract

Background
Autoimmune diseases are diseases caused by tissue damage caused by the body’s immune response to autoantibodies. Circular RNAs (CircRNAs) are a kind of special endogenous non-coding RNA that play a biological role by regulating gene transcription.

Methods
In this work, we searched the PubMed, Web of Science (SCIE), National Science and Technology Library (NSTL), and ScienceDirect Online (SDOL) databases to summarize the impact of circRNAs on autoimmune diseases, especially the results of circRNAs in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE).

Results
The study on the function of circRNAs and autoimmune diseases further deepened our understanding of the development and pathogenesis of autoimmune diseases. CircRNAs may act as miRNA sponges to regulate biological processes and affect the occurrence and development of autoimmune diseases. CircRNAs are closely related to the pathogenesis of RA and SLE and may become potential biomarkers for the diagnosis and treatment of RA and SLE.

Conclusion
CircRNAs play an important role in the pathogenesis of RA, SLE and other autoimmune diseases, and are expected to provide new biomarkers for the diagnosis and treatment of autoimmune diseases. However, the function and mechanism of circRNAs in autoimmune diseases need more comprehensive research.
The pros and cons of a global carbon tax at a single rate
Authors: Bacci Alessandro 
Published: December 2022

Abstract
During 2021, the International Monetary Fund (IMF), the Organisation for Economic Co-operation and Development (OECD) and the World Trade Organization (WTO), though differently, supported the idea that to effectively tackle climate change, it is necessary to introduce a global price on carbon emissions. Generally, a single-rate Global Carbon Tax (GCT) for all carbon emissions would probably be the best mechanism to tax carbon emissions while still favouring trading relationships globally within a WTO-compliant environment. However, implementing a single-rate GCT is extremely complicated. The IMF has proposed the establishment of an International Carbon Price Floor (ICPF), but it has recognized that to work the ICPF needs multiple carbon price minima according to the considered countries’ category of development level. This article, which is divided into seven sections, reflects on the idea of introducing a GCT. Section 1 briefs the introduction. Section 2 explains what the main reason is for putting a price on carbon emissions and clarifies how the two most important price-setting mechanisms, ie an ETS and a carbon tax, work. Section 3 explains how to set the carbon tax rate. Section 4 analyses the IMF’s GCT proposal. Section 5 discusses the benefits of a GCT, especially if based on a single tax rate. Section 6 reviews what, at least in the short term, the three main obstacles to the implementation of a single-rate GCT are. Section 7 concludes.
Two modes of Cue2-mediated mRNA cleavage with distinct substrate recognition initiate no-go decay
Authors: Tomomatsu Shota , Watanabe Atsuya , Tesina Petr , Hashimoto Satoshi , Ikeuchi Ken , Li Sihan , Matsuo Yoshitaka , Beckmann Roland , Inada Toshifumi 
Published: December 2022

Abstract
Ribosome collisions are recognized by E3 ubiquitin ligase Hel2/ZNF598, leading to RQC (ribosome-associated quality control) and to endonucleolytic cleavage and degradation of the mRNA termed NGD (no-go decay). NGD in yeast requires the Cue2 endonuclease and occurs in two modes, either coupled to RQC (NGDRQC+) or RQC uncoupled (NGDRQC−). This is mediated by an unknown mechanism of substrate recognition by Cue2. Here, we show that the ubiquitin binding activity of Cue2 is required for NGDRQC− but not for NGDRQC+, and that it involves the first two N-terminal Cue domains. In contrast, Trp122 of Cue2 is crucial for NGDRQC+. Moreover, Mbf1 is required for quality controls by preventing +1 ribosome frameshifting induced by a rare codon staller. We propose that in Cue2-dependent cleavage upstream of the collided ribosomes (NGDRQC−), polyubiquitination of eS7 is recognized by two N-terminal Cue domains of Cue2. In contrast, for the cleavage within collided ribosomes (NGDRQC+), the UBA domain, Trp122 and the interaction between Mbf1 and uS3 are critical.
Genome-wide inference reveals that feedback regulations constrain promoter-dependent transcriptional burst kinetics
Authors: Luo Songhao , Wang Zihao , Zhang Zhenquan , Zhou Tianshou , Zhang Jiajun 
Published: December 2022

AbstractGene expression in mammalian cells is highly variable and episodic, resulting in a series of discontinuous bursts of mRNAs. A challenge is to understand how static promoter architecture and dynamic feedback regulations dictate bursting on a genome-wide scale. Although single-cell RNA sequencing (scRNA-seq) provides an opportunity to address this challenge, effective analytical methods are scarce. We developed an interpretable and scalable inference framework, which combined experimental data with a mechanistic model to infer transcriptional burst kinetics (sizes and frequencies) and feedback regulations. Applying this framework to scRNA-seq data generated from embryonic mouse fibroblast cells, we found Simpson's paradoxes, i.e. genome-wide burst kinetics exhibit different characteristics in two cases without and with distinguishing feedback regulations. We also showed that feedbacks differently modulate burst frequencies and sizes and conceal the effects of transcription start site distributions on burst kinetics. Notably, only in the presence of positive feedback, TATA genes are expressed with high burst frequencies and enhancer–promoter interactions mainly modulate burst frequencies. The developed inference method provided a flexible and efficient way to investigate transcriptional burst kinetics and the obtained results would be helpful for understanding cell development and fate decision.
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
Authors: Keil Philipp , Wulf Alexander , Kachariya Nitin , Reuscher Samira , Hühn Kristin , Silbern Ivan , Altmüller Janine , Keller Mario , Stehle Ralf , Zarnack Kathi , Sattler Michael , Urlaub Henning , Sträßer Katja 
Published: December 2022

Abstract
RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein–RNA and protein–protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate multiple functions at once and likely impact the structural integrity of the large ribonucleoprotein particles (RNPs) these proteins function in. Using UV-induced RNA–protein crosslinking of entire cells, protein complex purification and mass spectrometric analysis, we identified &gt;100 in vivo RNA crosslinks in 16 nuclear mRNP components in Saccharomyces cerevisiae. For functional analysis, we chose Npl3, which displayed crosslinks in its two RNA recognition motifs (RRMs) and in the connecting flexible linker region. Both RRM domains and the linker uniquely contribute to RNA recognition as revealed by NMR and structural analyses. Interestingly, mutations in these regions cause different phenotypes, indicating distinct functions of the different RNA-binding domains. Notably, an npl3-Linker mutation strongly impairs recruitment of several mRNP components to chromatin and incorporation of other mRNP components into nuclear mRNPs, establishing a so far unknown function of Npl3 in nuclear mRNP assembly. Taken together, our integrative analysis uncovers a specific function of the RNA-binding activity of the nuclear mRNP component Npl3. This approach can be readily applied to RBPs in any RNA metabolic process.
Ratchet, swivel, tilt and roll: a complete description of subunit rotation in the ribosome
Authors: Hassan Asem , Byju Sandra , Freitas Frederico Campos , Roc Claude , Pender Nisaa , Nguyen Kien , Kimbrough Evelyn M , Mattingly Jacob M , Gonzalez Jr. Ruben L , de Oliveira Ronaldo Junio , Dunham Christine M , Whitford Paul C 
Published: December 2022

Abstract
Protein synthesis by the ribosome requires large-scale rearrangements of the ‘small’ subunit (SSU; ∼1 MDa), including inter- and intra-subunit rotational motions. However, with nearly 2000 structures of ribosomes and ribosomal subunits now publicly available, it is exceedingly difficult to design experiments based on analysis of all known rotation states. To overcome this, we developed an approach where the orientation of each SSU head and body is described in terms of three angular coordinates (rotation, tilt and tilt direction) and a single translation. By considering the entire RCSB PDB database, we describe 1208 fully-assembled ribosome complexes and 334 isolated small subunits, which span &gt;50 species. This reveals aspects of subunit rearrangements that are universal, and others that are organism/domain-specific. For example, we show that tilt-like rearrangements of the SSU body (i.e. ‘rolling’) are pervasive in both prokaryotic and eukaryotic (cytosolic and mitochondrial) ribosomes. As another example, domain orientations associated with frameshifting in bacteria are similar to those found in eukaryotic ribosomes. Together, this study establishes a common foundation with which structural, simulation, single-molecule and biochemical efforts can more precisely interrogate the dynamics of this prototypical molecular machine.
Kinetics of elementary steps in loop-mediated isothermal amplification (LAMP) show that strand invasion during initiation is rate-limiting
Authors: Dangerfield Tyler L , Paik Inyup , Bhadra Sanchita , Johnson Kenneth A , Ellington Andrew D 
Published: December 2022

Abstract
Loop-mediated isothermal amplification (LAMP) has proven to be easier to implement than PCR for point-of-care diagnostic tests. However, the underlying mechanism of LAMP is complicated and the kinetics of the major steps in LAMP have not been fully elucidated, which prevents rational improvements in assay development. Here we present our work to characterize the kinetics of the elementary steps in LAMP and show that: (i) strand invasion / initiation is the rate-limiting step in the LAMP reaction; (ii) the loop primer plays an important role in accelerating the rate of initiation and does not function solely during the exponential amplification phase and (iii) strand displacement synthesis by Bst-LF polymerase is relatively fast (125 nt/s) and processive on both linear and hairpin templates, although with some interruptions on high GC content templates. Building on these data, we were able to develop a kinetic model that relates the individual kinetic experiments to the bulk LAMP reaction. The assays developed here provide important insights into the mechanism of LAMP, and the overall model should be crucial in engineering more sensitive and faster LAMP reactions. The kinetic methods we employ should likely prove useful with other isothermal DNA amplification methods.