bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cellulase–lactic acid bacteria synergy action regulates silage fermentation of woody plant
Authors: Du Zhumei , Yamasaki Seishi , Oya Tetsuji , Cai Yimin 
Published: August 2023

Abstract
Background
Feed shortage is an important factor limiting livestock production in the world. To effectively utilize natural woody plant resources, we used wilting and microbial additives to prepare an anaerobic fermentation feed of mulberry, and used PacBio single-molecule real-time (SMRT) sequencing technology to analyse the “enzyme–bacteria synergy” and fermentation mechanism.

Results
The fresh branches and leaves of mulberry have high levels of moisture and nutrients, and also contain a diverse range of epiphytic microorganisms. After ensiling, the microbial diversity decreased markedly, and the dominant bacteria rapidly shifted from Gram-negative Proteobacteria to Gram-positive Firmicutes. Lactic acid bacteria (LAB) emerged as the dominant microbial population, resulting in increased in the proportion of the carbohydrate metabolism and decreased in the proportion of the amino acid and “global and overview map” (GOM) metabolism categories. The combination of cellulase and LAB exhibited a synergistic effect, through which cellulases such as glycanase, pectinase, and carboxymethyl cellulase decomposed cellulose and hemicellulose into sugars. LAB converted these sugars into lactic acid through the glycolytic pathway, thereby improving the microbial community structure, metabolism and fermentation quality of mulberry silage. The GOM, carbohydrate metabolism, and amino acid metabolism were the main microbial metabolic categories during ensiling. The presence of LAB had an important effect on the microbial community and metabolic pathways during silage fermentation. A “co-occurrence microbial network” formed with LAB, effectively inhibiting the growth of harmful microorganisms, and dominating the anaerobic fermentation process.

Conclusions
In summary, PacBio SMRT was used to accurately analyse the microbial network information and regulatory mechanism of anaerobic fermentation, which provided a scientific basis for the study of woody silage fermentation theory. This study reveals for the first time the main principle of the enzyme–bacteria synergy in a woody silage fermentation system, which provides technical support for the development and utilization of woody feed resources, and achieves sustainable livestock production.
Prevalence of treated patients with Alzheimer’s disease: current trends and COVID-19 impact
Authors: Olazarán Javier , Carnero-Pardo Cristóbal , Fortea Juan , Sánchez-Juan Pascual , García-Ribas Guillermo , Viñuela Félix , Martínez-Lage Pablo , Boada Mercè 
Published: August 2023

Abstract
Background
There are few updated studies on the prevalence and management of Alzheimer’s disease (AD), which could be underdiagnosed or undertreated. The COVID-19 pandemic may have worsened the deficiencies in the diagnosis and treatment of these patients. Electronic medical records (EMR) offer an opportunity to assess the impact and management of medical processes and contingencies in the population.

Objective
To estimate AD prevalence in Spain over a 6-year period, based on treated patients, according to usual clinical practice. Additionally, to describe the management of AD-treated patients and the evolution of that treatment during the 2020 COVID-19 pandemic.

Methods
Retrospective study using the Spanish IQVIA EMR database. Patients treated with donepezil, galantamine, rivastigmine, and/or memantine were included in the study. Annual AD prevalence (2015–2020) was estimated and extrapolated to the national population level. Most frequent treatments and involved specialties were described. To assess the effect of COVID-19, the incidence of new AD cases in 2020 was calculated and compared with newly diagnosed cases in 2019.

Results
Crude AD prevalence (2015–2020) was estimated at 760.5 per 100,000 inhabitants, and age-standardized prevalence (2020) was 664.6 (male 595.7, female 711.0). Monotherapy was the most frequent way to treat AD (86.2%), in comparison with dual therapy (13.8%); rivastigmine was the most prescribed treatment (37.3%), followed by memantine (36.4%) and donepezil (33.0%). Rivastigmine was also the most utilized medication in newly treated patients (46.7%), followed by donepezil (29.8%), although donepezil persistence was longer (22.5 vs. 20.6 months). Overall, donepezil 10 mg, rivastigmine 9.5 mg, and memantine 20 mg were the most prescribed presentations. The incidence rate of AD decreased from 148.1/100,000 (95% confidence interval [CI] 147.0–149.2) in 2019 to 118.4/100,000 (95% CI 117.5–119.4) in 2020.

Conclusions
The obtained prevalence of AD-treated patients was consistent with previous face-to-face studies. In contrast with previous studies, rivastigmine, rather than donepezil, was the most frequent treatment. A decrease in the incidence of AD-treated patients was observed during 2020 in comparison with 2019, presumably due to the significant impact of the COVID-19 pandemic on both diagnosis and treatment. EMR databases emerge as valuable tools to monitor in real time the incidence and management of medical conditions in the population, as well as to assess the health impact of global contingencies and interventions.
Presence and role of viruses in anaerobic digestion of food waste under environmental variability
Authors: Fan Lu , Peng Wei , Duan Haowen , Lü Fan , Zhang Hua , He Pinjing 
Published: August 2023

Abstract
Background
The interaction among microorganisms in the anaerobic digestion of food waste (ADFW) reactors lead to the degradation of organics and the recycling of energy. Viruses are an important component of the microorganisms involved in ADFW, but are rarely investigated. Furthermore, little is known about how viruses affect methanogenesis.

Results
Thousands of viral sequences were recovered from five full-scale ADFW reactors. Gene-sharing networks indicated that the ADFW samples contained substantial numbers of unexplored anaerobic-specific viruses. Moreover, the viral communities in five full-scale reactors exhibited both commonalities and heterogeneities. The lab-scale dynamic analysis of typical ADFW scenarios suggested that the viruses had similar kinetic characteristics to their prokaryotic hosts. By associating with putative hosts, a majority of the bacteria and archaea phyla were found to be infected by viruses. Viruses may influence prokaryotic ecological niches, and thus methanogenesis, by infecting key functional microorganisms, such as sulfate-reducing bacteria (SRB), syntrophic acetate-oxidizing bacteria (SAOB), and methanogens. Metabolic predictions for the viruses suggested that they may collaborate with hosts at key steps of sulfur and long-chain fatty acid (LCFA) metabolism and could be involved in typical methanogenesis pathways to participate in methane production.

Conclusions
Our results expanded the diversity of viruses in ADFW systems and suggested two ways that viral manipulated ADFW biochemical processes.
Validation of Play Fairway Analysis of the geothermal potential of Camas Prairie, south-central Idaho, by an exploration well
Authors: Lachmar Thomas E. , Neupane Ghanashyam , Garg Sabodh K. , Dobson Patrick F. , Smith Connor J. , Newell Dennis L. , Shervais John W. , Evans James P. , Mink Leland L. 
Published: August 2023

AbstractPlay Fairway Analysis (PFA) methodology was adapted for geothermal exploration at Camas Prairie, Idaho. Geophysical data, structural and geologic mapping, volcanic rock ages and vent locations, and the distribution of thermal springs and wells all indicated a relatively high geothermal potential along the southern margin of the Prairie. An exploration well (USU Camas-1) was drilled to a depth of 618.3 m to validate the PFA. A permeable zone was encountered at ~ 357.5 m with a maximum measured temperature of ~ 80 °C, which was suppressed following the injection of cold water. A moderate transmissivity of ~ 0.25–1 cm2/s estimated from an injection test as well a seasonal artesian flow at ~ 0.7 L/s corroborate the presence of a permeable zone. The existence of a lacustrine clay seal was confirmed near the bottom of the basin-fill sediment occupying the upper 314 m of the well. Geothermometers suggest the USU Camas-1 well water equilibrated at a reservoir temperature of ~ 120 °C. Based on the locations of both thermal and cold wells, geothermal fluids appear to be flowing upward along one or both of two fault systems. The presence of young basalts and elevated helium isotope ratios suggest that the heat source of Camas Prairie is magmatic. However, the faults may be acting as a conduit for geothermal fluids to rise from great depth without a shallow magmatic source being present. Camas Prairie is a promising area for geothermal development, but the relatively low reservoir temperatures indicate this resource may not be suitable for electric generation. Perhaps the best use would be for heating.
Hydroxyapatite nanoparticles: an alternative to conventional phosphorus fertilizers in acidic culture media
Authors: Noruzi Masumeh , Hadian Parvin , Soleimanpour Leila , Ma’mani Leila , Shahbazi Karim 
Published: August 2023

Abstract
Background
Traditional phosphorus fertilizers generally have low efficiencies due to their immobilization in soil, and a large part of these fertilizers are not plant-available. Also, phosphorus resources are non-renewable. In recent years, a great deal of attention has been paid to nanofertilizers because of their slow or controlled release and also their very small particle size which increases the solubility and uptake of nanoparticles in plant. Hydroxyapatite nanoparticles are of great importance as phosphorus nanofertilizer thanks to their very low toxicity, biocompatibility, and the fact that products obtained from their degradation, i.e., phosphate and calcium ions, are naturally available in soils.

Results
In this study, hydroxyapatite nanoparticles were synthesized using the wet chemical precipitation method in three formulations and characterized with various techniques including electron microscopy, atomic force microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and elemental analysis. Chemical and microscopic analyses showed that phosphorus was distributed in different parts of the wheat (Triticum aestivum L.) plant. To investigate the fertilizing effects of the nanoparticles, hydroxyapatite nanoparticles were used in different culture media including alkaline soil, acidic soil, the mixture of peat moss and perlite, and cocopeat. Based on our observations, hydroxyapatite nanoparticles showed fertilizing properties in all media. However, fertilizing potential strongly depended on the culture media. HAP nanoparticles demonstrated a high potential to be used as a fertilizer in acidic media. Nevertheless, only a slight fertilizing effect was observed in alkaline soils. Furthermore, the findings of our study showed fertilizing properties of powder hydroxyapatite nanoparticles without the need to convert them to suspension. Moreover, hydroxyapatite nanoparticles in all the three formulations showed low toxicity in such a way that their toxicity was even less than that of triple super phosphate.

Conclusions
Hydroxyapatite nanoparticles in both suspension and powder forms can be considered an alternative to conventional phosphorus fertilizers in acidic culture media. Our study revealed that hydroxyapatite nanoparticles were likely dissolved in the culture media and absorbed by plant mainly in the phosphate form.

Graphical Abstract
The impact of sedative and vasopressor agents on cerebrovascular reactivity in severe traumatic brain injury
Authors: Froese Logan , Hammarlund Emma , Åkerlund Cecilia A. I. , Tjerkaski Jonathan , Hong Erik , Lindblad Caroline , Nelson David W. , Thelin Eric P. , Zeiler Frederick A. 
Published: August 2023

Abstract
Background
The aim of this study is to evaluate the impact of commonly administered sedatives (Propofol, Alfentanil, Fentanyl, and Midazolam) and vasopressor (Dobutamine, Ephedrine, Noradrenaline and Vasopressin) agents on cerebrovascular reactivity in moderate/severe TBI patients. Cerebrovascular reactivity, as a surrogate for cerebral autoregulation was assessed using the long pressure reactivity index (LPRx). We evaluated the data in two phases, first we assessed the minute-by-minute data relationships between different dosing amounts of continuous infusion agents and physiological variables using boxplots, multiple linear regression and ANOVA. Next, we assessed the relationship between continuous/bolus infusion agents and physiological variables, assessing pre-/post- dose of medication change in physiology using a Wilcoxon signed-ranked test. Finally, we evaluated sub-groups of data for each individual dose change per medication, focusing on key physiological thresholds and demographics.

Results
Of the 475 patients with an average stay of 10 days resulting in over 3000 days of recorded information 367 (77.3%) were male with a median Glasgow coma score of 7 (4–9). The results of this retrospective observational study confirmed that the infusion of most administered agents do not impact cerebrovascular reactivity, which is confirmed by the multiple linear regression components having p value > 0.05. Incremental dose changes or bolus doses in these medications in general do not lead to significant changes in cerebrovascular reactivity (confirm by Wilcoxon signed-ranked p value > 0.05 for nearly all assessed relationships). Within the sub-group analysis that separated the data based on LPRx pre-dose, a significance between pre-/post-drug change in LPRx was seen, however this may be more of a result from patient state than drug impact.

Conclusions
Overall, this study indicates that commonly administered agents with incremental dosing changes have no clinically significant influence on cerebrovascular reactivity in TBI (nor do they impair cerebrovascular reactivity). Though further investigation in a larger and more diverse TBI patient population is required.
Feasibility and acceptability of a new web-based cognitive training platform for cognitively healthy older adults: the breakfast task
Authors: Sanz Simon Sharon , Ben-Eliezer Daniel , Pondikos Maria , Stern Yaakov , Gopher Daniel 
Published: August 2023

Abstract
Background
Developing efficient cognitive training for the older population is a major public health goal due to its potential cognitive benefits. A promising training target is executive control, critical for multitasking in everyday life. The aim of this pilot study was to establish the feasibility and acceptability of the Breakfast Task training in older adults, a new web-based cognitive training platform that simulates real-life multitasking demands.

Methods
A community-based sample of 24 cognitively healthy participants aged between 60 and 75 (M = 69.12, SD = 3.83) underwent 5-session cognitive training protocol, delivered online. Each session lasted 45 min and occurred twice a week at participant’s homes. Performance was recorded, and participants completed questionnaires at baseline and after the intervention.

Results
Feasibility metrics showed overall high recruitment (82.7%), adherence and retention rates (100%). Acceptability was considered good based on participant’s quantitative and qualitative responses. On average, participants rated the game as interesting, enjoyable and did not report difficulties in accessing the game online without supervision or in understanding the instructions. Participants showed a learning curve across sessions, suggesting improvement in the game outcomes and potential benefits from the emphasis change training approach. The study identified relevant areas that need improvements and adjustments, such as technical issues, session’s structure, and dose.

Conclusions
The findings provide preliminary support for the feasibility and acceptability of the web-based Breakfast Task training platform in cognitively healthy older adults. Results suggest the value of further research to investigate the Breakfast Task training features and dose-response relationship, as well as its potential efficacy in older adults via larger randomized controlled trials.

Trial registration
ClinicalTrials.gov: NCT04195230 (Registered 11 December 2019).
Pipeline versus Tubridge in the treatment of unruptured posterior circulation aneurysms
Authors: Jin Hengwei , Lv Jian , Meng Xiangyu , Liu Xinke , He Hongwei , Li Youxiang 
Published: August 2023

Abstract
Background
To compare the safety and efficacy of pipeline embolization device (PED) and Tubridge flow diverter (TFD) for unruptured posterior circulation aneurysms.

Methods
Posterior aneurysm patients treated with PED or TFD between January, 2019, and December, 2021, were retrospectively reviewed. Patients’ demographics, aneurysm characteristics, treatment details, complications, and follow-up information were collected. The procedural-related complications and angiographic and clinical outcome were compared.

Results
A total of 107 patients were involved; PED was applied for 55 patients and TFD for 52 patients. A total of 9 (8.4%) procedural-related complications occurred, including 4 (7.3%) in PED group and 5 (9.6%) in TFD group. During a mean of 10.3-month angiographic follow-up for 81 patients, complete occlusion was achieved in 35 (85.4%) patients in PED group and 30 (75.0%) in TFD group. The occlusion rate of PED group is slightly higher than that of TFD group. A mean of 25.0-month clinical follow-up for 107 patients showed that favorable clinical outcome was achieved in 53 (96.4%) patients in PED group and 50 (96.2%) patients in TFD group, respectively. No statistical difference was found in terms of procedural-related complications (p = 0.737), occlusion rate (p = 0.241), and favorable clinical outcome (0.954) between groups.

Conclusions
The current study found no difference in complication, occlusion, and clinical outcome between PED and TFD for unruptured PCAs.
National hepatitis registry in Pakistan: a dire need for hepatitis surveillance and control
Authors: Farhan Muhammad , Fazal Faizan , Dave Tirth , Butt Armeen , Basit Jawad , Maqbool Shahzaib 
Published: August 2023

AbstractHepatitis is a major public health issue in Pakistan, with an estimated 11.55% prevalence of HCV infection in the adult population. The country ranks second globally in terms of hepatitis C virus (HCV) infections, with approximately one in every 20 Pakistanis already infected. The mortality rates due to HBV and HCV stand at 563,000 and 366,000 annually, respectively. However, the absence of a national registry or database system and the lack of coordination among provinces pose significant obstacles in combating this disease effectively. To address this issue, the establishment of a centralized national database registry is crucial, allowing comprehensive analysis, tracking of hepatitis prevalence, and identification of high-risk areas for targeted interventions. By fostering collaboration among provinces, the government, and non-governmental organizations, the registry would facilitate joint decision-making, minimize duplication of efforts, and address inconsistencies in diagnosis and treatment. Collaborating with student-run organizations and leveraging enhanced laboratory capacities post-COVID era can strengthen the hepatitis control program. The centralized approach and unified efforts are necessary to achieve the goal of a hepatitis-free Pakistan, where a healthier future can be realized.
Graded prioritisation of targets in search: reward diminishes the low prevalence effect
Authors: Hadjipanayi Veronica , Ludwig Casimir J. H. , Kent Christopher 
Published: August 2023

AbstractIn many real-life contexts, observers are required to search for targets that are rarely present (e.g. tumours in X-rays; dangerous items in airport security screenings). Despite the rarity of these items, they are of enormous importance for the health and safety of the public, yet they are easily missed during visual search. This is referred to as the prevalence effect. In the current series of experiments, we investigate whether unequal reward can modulate the prevalence effect, in amultipletarget search task. Having first established the impact of prevalence (Experiment 1) and reward (Experiment 2) on how efficiently participants can find one of several targets in the current paradigm, we then combined the two forms of priority to investigate their interaction. Anunequalreward distribution (where lower prevalence items are more rewarded; Experiment 3) was found to diminish the effect of prevalence, compared to anequalreward distribution (Experiment 4) as indicated by faster response times and fewer misses. These findings suggest that when combined with an unequal reward distribution, the low prevalence effect can be diminished.
Effect of a waste management intervention program on knowledge, attitude, and practice (KAP) of nurses and housekeepers: a quasi-experimental study, Egypt
Authors: Khashaba Eman , El-Gilany Abdel Hady , Denewar Khadija 
Published: August 2023

Abstract
Background
Improper healthcare waste management practice is alarming in developing countries because resources are inadequate and waste management is often delegated to poorly educated and untrained laborers. This study aimed to compare the pre-KAP versus post-KAP towards the waste management program for nurses and housekeepers. In addition, it aimed to explore possible factors affecting the pre- and post-KAP in Mansoura Emergency University Hospital, Egypt.

Subjects and methods
One hundred thirty-three newly employed nurses, housekeepers, and those who need refreshment training as nominated by head nurses and link occupational health and safety nurses in the hospital were recruited for the study. The study’s intervention included multiple training sessions using a PowerPoint presentation in Arabic with appropriate illustrations followed by an open discussion. An Arabic self-administered questionnaire containing demographic and occupational history, knowledge (27 questions), attitude (10 questions), and practice (9 questions) was used pre- and post-intervention.

Results
The overall KAP scores among the studied healthcare workers were significantly higher after the intervention. The pre- and post-knowledge scores were significantly different with respect to education, job description, and duration of employment (p < 0.05). The post-attitude scores were significantly different with respect to education and job description only (p < 0.05). The total pre-practice scores were significantly different with respect to education and job description (p < 0.05). However, the post-practice scores were significantly different with respect to sex, age, education, and job description (p < 0.05).

Conclusion
There was a significant improvement in the KAP scores post-intervention. The post-knowledge and attitude scores were significantly better in nurses and participants with a higher education. The post-practice score was significantly better for females, participants with an age ≥ 30 years, higher education, and nursing jobs. The combination of training and supervision was crucial for the success of waste management programs. Higher education levels are required for housekeepers to be capable of gaining better knowledge, follow rules, and be ready for any challenges in the future.