bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA CCAT1 facilitates the progress of gastric cancer via the PTBP1/glycolysis axis
Authors: wang huixia , Liu Qingwei , Zhao Lianmei , Dai Suli , Tian Guo , Wei Xintong , Li Xiaoya , Zhang Cong , Shan Baoen 
Published: April 2023

Abstract
Background: Gastric cancer (GC) is one of the most common malignant tumors of the digestive system. As a hallmark of cancer, energy-related metabolic reprogramming was manipulated by various factors, including lncRNAs. It has been shown that lncRNA CCAT1 is a key regulator involved in tumor development. Nevertheless, the exact underlying molecular mechanisms by which lncRNA CCAT1 acts in GC metabolic reprogramming are yet to be elucidated.
Methods: The expression of CCAT1 in GC tissues, serum, and exosome that was isolated from plasma and GC cell lines were detected by qRT-PCR. The gain and loss-function assays were performed to explore the role of CCAT1 on GC cells. Xenograft tumor formation models in nude mice were performed to estimate the proliferation of GC cells with CCAT1 stably knocking down in vivo. The proteins interacting with CCAT1 were first analyzed by online databases and further confirmed by RNA pull-down and RNA immunoprecipitation (RIP) assays. The expression of glycolytic signaling pathway-related proteins were probed using western blotting and immunohistochemistry (IHC).
Results: In this study, we identified that CCAT1 was remarkably enhanced in the tissues, serum, and plasma exosomes of GC patients as well as in GC cell lines. Functional experiments showed that knockdown of CCAT1 significantly reduced the proliferation, migration and invasion of GC cells in vitro and in vivo, and also decreased glycolytic rate and the expression of glycolytic enzymes in GC cells, whereas overexpression of CCAT1 had opposing effects. Mechanically, CCAT1 interacted with PTBP1 and maintained its stability by inhibiting the ubiquitin-mediated degradation. As a critical splicing factor, PTBP1 induced a switch from PKM1 to PKM2, leading to an increase in the glycolysis of GC cells and ultimately promoting GC progression.
Conclusions: Our study exhibited that CCAT1 contributed to GC proliferation, migration and invasion via PTBP1 / glycolysis axis, making it a potential biomarker and therapeutic target in GC patients.
Metabolic functional profiles of microbial communities in methane production systems treating winery wastewater
Authors: Vital-Jacome Miguel , Carrillo-Reyes Julián , Buitrón Germán 
Published: April 2023

Abstract
Winery effluents are agro-industrial wastes that can be treated to produce methane in anaerobic digestion systems. Understanding how the process configuration and operating conditions affect the microbial communities and their metabolism is essential to improve the performance of these systems. This work aimed to compare the taxonomic and functional profiles of four process configurations of an anaerobic digestion system treating winery effluents. Taxonomic profiles were obtained by sequencing the 16S rRNA gene, and the Tax4Fun2 package was used to predict the functional profiles. Different genera predominated under three configurations in the acidogenic reactor, Sporolactobacillus, Prevotella and Acetobacter, respectively; however, the analysis indicated high functional redundancy among configurations. Methane production by the acetoclastic pathway was mainly related to Methanosaeta in the single-stage configuration and related to five different archaea genera in the two-stage configuration. Syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis predominated under thermophilic conditions, where Methanothermobacter had up to 65% of the relative abundance. The two-stage configuration was more stable due to the higher microbial diversity and functional redundancy than the single-stage; the microbial community in mesophilic conditions also had higher microbial diversity and functional redundancy than in thermophilic conditions; finally, applying harsher operating conditions reduces the microbial diversity and the stability of the process. This analysis provided valuable knowledge to understand and improve methane production in anaerobic digestion systems.
Association between intrarenal venous flow from Doppler ultrasonography and acute kidney injury in patients with sepsis in critical care: A prospective, exploratory observational study
Authors: Fujii Kenichiro , Nakayama Izumi , Izawa Junichi , Ishihara Takuma , Iida Noriko , Seo Yoshihiro , Yamamoto Masayoshi , Uenishi Norimichi , Terasawa Teruhiko , Iwata Mitsunaga 
Published: April 2023

Abstract
Background:
Intrarenal venous flow (IRVF) patterns assessed using Doppler renal ultrasonography are real-time bedside visualizations of renal vein hemodynamics. Although this technique has the potential to detect renal congestion during sepsis resuscitation, there have been few studies on this method. We aimed to examine the relationship between IRVF patterns, clinical parameters, and outcomes in critically ill adult patients with sepsis. We hypothesized that discontinuous IRVF was associated with elevated central venous pressure (CVP) and subsequent acute kidney injury (AKI) or death.
Methods:
We conducted a prospective observational study in two tertiary-care hospitals, enrolling adult patients with sepsis who stayed in the intensive care unit for at least 24 h, had central venous catheters placed, and received invasive mechanical ventilation. Renal ultrasonography was performed at the bedside, and IRVF patterns (discontinuous vs. continuous) were confirmed by a blinded assessor. The primary outcome was CVP obtained at the time of renal ultrasonography. We also repeatedly assessed a composite of Kidney Disease Improving Global Outcomes of Stage 3 AKI or death over the course of a week as a secondary outcome. The association of IRVF patterns with CVP was examined using Student's t-test (primary analysis) and that with composite outcomes was assessed using a generalized estimating equation analysis, to account for intra-individual correlations. A sample size of 32 was set in order to detect a 5-mmHg difference in CVP between IRVF patterns.
Results:
Of the 38 patients who met the eligibility criteria, 22 (57.9%) showed discontinuous IRVF patterns that suggested blunted renal venous flow. IRVF patterns were not associated with CVP (discontinuous flow group: mean 9.24 cm H2O [standard deviation: 3.19], continuous flow group: 10.65 cm H2O [standard deviation: 2.53], p=0.154). By contrast, the composite outcome incidence was significantly higher in the discontinuous IRVF pattern group (odds ratio: 9.92; 95% confidence interval: 2.12–46.58, p=0.004).
Conclusions:
IRVF patterns were not associated with CVP but were associated with subsequent AKI in critically ill adult patients with sepsis. IRVF may be useful for capturing renal congestion at the bedside that is related to clinical patient outcomes.
Debranching-first followed by aortic arch replacement with frozen elephant trunk
Authors: Suzuki Ryo , Akita Masafumi , Miyazaki Suguru , Shimano Ryo 
Published: April 2023

Abstract
Background: Diffuse thoracic aortic aneurysm has been a challenge for cardiovascular surgeons as aortic arch and descending aortic aneurysm should be treated simultaneously somehow. The total arch replacement (TAR) using a frozen elephant trunk (FET) allows them to treat aortic arch and descending aortic pathology at once via median sternotomy. Besides, extra-anatomical bypass performed between the left common carotid artery (CCA) and subclavian artery (SCA) prior to TAR allowed further proximalization of FET prosthesis and facilitated distal anastomosis of TAR and spared the demanding Left subclavian artery (LSA) anastomosis in deep pericardial space.
We investigated the efficacy of this debranching-first technique followed by total arch replacement using a frozen elephant trunk as a two-stage operation for extensive thoracic aortic aneurysm among high-risk patients.
Methods: Forty-nine consecutive patients with diffuse degenerative aneurysms from the aortic arch to the descending aorta or chronic aortic dissection receiving left common carotid to subclavian artery bypass followed by total arch replacement using a frozen elephant trunk and possible subsequent thoracic endovascular aortic repair between 2016 and 2021 were analysed. The baseline characteristics and clinical outcomes were demonstrated. Overall survival rate and 5-year aortic event-free survival, aortic reintervention rates were analysed.
Results: The average score of European System for Cardiac Operative Risk Evaluation (EuroSCORE II) was 4.7±2.5. The operative mortality rate was 4.1%, with no paraplegia events. The five-year overall survival, cumulative aortic related mortality were 76.8%, 2%, respectively. The five-year overall cumulative aortic reintervention rates including intended intervention were 31.3%. The 5-year cumulative rate of non-intended reintervention was 4.5%.
Conclusions: The assessed technique enables a less invasive and less technically demanding surgery with reasonable outcomes. The 5-year aortic event-free survival and reintervention rates were acceptable, suggesting that multiple stages of alternative open and endovascular interventions, such as this technique, may reduce the morbidity and mortality rates of high-risk patients with diffuse thoracic aortic aneurysm.
Clinical registration number: 2022001
Therapeutic yield of extensive molecular profiling in cholangiocarcinoma: a retrospective single center study.
Authors: Vancanneyt Justine , Wilmsen Bie , Luyten Caroline , Verslype Chris , Cutsem Eric , Roskams Tania , Tejpar Sabine , Bempt Isabelle Vanden , Dekervel Jeroen 
Published: April 2023

Abstract
Introduction: Current available systemic therapies for advanced cholangiocarcinoma (CCA) are of limited effectiveness and prognosis is poor. Recently, introduction of next-generation sequencing (NGS) technologies led to a better understanding of the genetic pathophysiology and consequently, identification of molecular alterations for targeted treatment.
Aim: To determine the proportion of actionable alterations using extensive molecular profiling in a routine diagnostic setting and to study the effect of targeted treatment on disease control.
Methods: Results of extensive molecular testing by either FoundationOne NGS or an in-house developed 96 cancer gene panel were retrospectively collected from patients with locally advanced or metastatic CCA diagnosed between 01/12/2018 and 01/08/2021 in a single center. Gene variants were classified according to ESCAT and correlated with efficacy endpoints.
Results: Of 125 patients included, 65 patients had an intrahepatic CCA (iCCA).
FGFR2 fusions and IDH1/BAP1 mutations were more frequent in iCCA, while KRAS and SMAD4 mutations were predominant in extrahepatic CCA (eCCA). Targetable alterations (ESCAT tiers I-IV) were identified in 73,6% of patients. Overall survival was significantly better for higher tiers regardless of treatment.
Thirteen patients (10.4%) received targeted treatment based on molecular profiling, with a median progression free survival (PFS) of 7,3 months.
Conclusions: Extensive molecular characterization led to the identification of targetable and potentially targetable alterations in a significant proportion of patients with locally advanced or metastatic CCA.
We confirmed the association between higher ESCAT tier and benefit of a targeted treatment.
Molecular analysis should therefore be considered in all patients fit enough for systemic treatment.
Some applications of renormalization group to few-body problems in EFT
Authors: Mindadze Bidzina , Epelbaum Evgeny , Gegelia Jambul 
Published: April 2023

Abstract
General aspects of applying renormalization group in perturbative calculations are briefly discussed. Next, an application of the Wilsonian renormalization group with multitude of cutoff parameters to halo EFT for P-wave shallow states is considered.
IRFSC: A high-efficiency hybrid model Image Retrieval Based on Feature Selection Using Continuous Learning Process
Authors: Lande Milind Vijayrao , Ridhorkar Sonali 
Published: April 2023

Abstract
Design of high-efficiency feature representation and ranking models is required for retrieval of images based on colour, texture, shape, and other visual aspects. These models must be able to increase retrieval precision while reducing the amount of error and delay required for ranking procedures. Low complexity models can run more quickly, but they are limited in their retrieval performance because they do not exhibit higher retrieval rates. This essay suggests designing a novel hybrid model for high-efficiency feature selection-based picture retrieval using a continuous learning approach to address these problems. A hybrid Elephant Herding Optimization (EHO) & Particle Swarm Optimization (PSO) layer is used in the model's initial extraction of large feature sets from multimodal images in order to continually maximize inter-class feature variance levels. These ranks are post-processed using an incremental optimization method based on Q-Learning, which supports in the continuous optimization of image data sets. As compared to recently proposed state-of-the-art models, the suggested model is able to preserve reduced delay while improving retrieval accuracy by 0.07%, precision by 10.5%, and recall by 3.60%. As a result, the proposed model can be used for a wide range of real-time use cases.
Optimal pension fund management with stochastic additional contribution rate in a defined contribution pension plan
Authors: Nkeki Charles , Ibe C. B. 
Published: April 2023

Abstract
This paper examines an optimal pension fund management in which a pension plan member (PPM) make a flow of contributions that comprises of two parts: mandatory contribution with fixed contribution rate, and additional voluntary contribution (AVC) with stochastic contribution rate. The mandatory part is a fixed fraction of a PPM's stochastic salary while the latter part is assume to be stochastic with contribution growth rate (CGR) and volatility depending on the contribution rate, the salary and the wealth processes of a PPM over time. The sum of the two contribution rates make up the total stochastic contribution rate of a PPM. Two asset classes are consider: the risky and the riskless assets. The latter pays a stochastic interest rate that follows uncontrolled Vasisek model. Also, it is assume that the PPM consume some portion of his or her wealth continuously over time. This paper aims at determining the optimal investment, optimal consumption rate, the optimal contribution rate and effect of AVC rate on the investment portfolio. The optimal investment portfolio, optimal contribution rate, optimal consumption rate and explicit expression of the CGR of a PPM's contribution rate are obtained. We found that the investment portfolio depend inversely on the contribution rate and the interest rate, and directly on the contribution of a PPM. The intertemporary hedging portfolio strategies with respect to contribution rate, contribution process and interest rate are obtained. Furthermore, we found that the CGR depend on the optimal wealth, optimal contribution rate, interest rate, salary, investment risk, salary risk, contribution risk, interest rate risk, and coefficient of constant relative risk averse of a PPM. We also found that the CGR depend directly on the salary and the contribution processes and inversely on the contribution rate and interest rate of a PPM over time. Also, we found that the CGR react to changes in the salary process and its risk, wealth process, contribution rate and contribution risk, interest rate and interest rate risk, and coeffecient of risk averse.
Electrodermal and central measures of the phasic orienting reflex (OR)
Authors: Barry Robert J. , Steiner-Lim Genevieve Z. , Cave Adele E. , De Blasio Frances M. , MacDonald Brett 
Published: April 2023

Abstract
Although the P300 event-related potential (ERP) is the most likely central measure of Sokolov’s Orienting Reflex (OR), there are few systematic comparisons with the skin conductance response (SCR), the “gold standard” electrodermal OR measure. We examine habituation, stimulus significance, and inter-stimulus interval (ISI) effects in SCRs and components of the P300 from single-trial ERPs in an auditory dishabituation paradigm. Single trial ERP components were separated by temporal principal components analysis, and five components of the P300 were examined as potential phasic OR measures: P3a, P3b, Novelty P3, and two Slow Waves (SW1, SW2). Across the factors of ISI and significance, SCRs showed decrement over trials, recovery at a deviant, and dishabituation at the subsequent standard. This general pattern was not present in any of the components of the P300. SCRs were also larger to significant stimuli and at the long ISI; effects differed between P300 components. The electrodermal SCR showed the complete profile over trials expected of the phasic OR, and was enhanced by stimulus significance, confirming it as the model measure of Sokolov’s phasic OR. Components of the P300 failed to match this profile, but instead appear to reflect different aspects of the stimulus processing involved in OR elicitation.
Association between SARS-CoV-2 variants and post COVID-19 condition: findings from a longitudinal cohort study in the Belgian adult population
Authors: Nguyen Huyen Thi Khanh , Laura Cornelissen , Diego Castanares-Zapatero , De Pauw Robby , Cauteren Dieter Van , Stefaan Demarest , Sabine Drieskens , Devleesschauwer Brecht , De Ridder Karin , Charafeddine Rana , Smith Pierre 
Published: April 2023

Abstract
Background: While many studies on the determinants of post-COVID-19 conditions (PCC) have been conducted, little is known about the relationship between SARS-CoV-2 variants and PCC. This study aimed to assess the association between different SARS-CoV-2 variants and the probability of having PCC three months after the infection.
Methods: This study was a longitudinal cohort study conducted between April 2021 and September 2022 in Belgium. In total, 8,238 adults with a confirmed SARS-CoV-2 infection were followed up between the time of their infection and three months later. The primary outcomes were the PCC status three months post infection and seven PCC symptoms categories (neurocognitive, autonomic, gastrointestinal, respiratory, musculoskeletal, anosmia and/or dysgeusia, and other manifestations). The main exposure variable was the type of SARS-CoV-2 variants (i.e. Alpha, Delta, and Omicron), extracted from national surveillance data. The association between the different SARS-CoV-2 variants and PCC as well as PCC symptoms categories was assessed using multivariable logistic regression.
Results: The proportion of PCC among participants infected during the Alpha, Delta, and Omicron-dominant periods was significantly different and respectively 50%, 50%, and 37%. Participants infected during the Alpha- and Delta-dominant periods had a significantly higher odds of having PCC than those infected during the Omicron-dominant period (OR = 1.61, 95% confidence interval [CI] = 1.33–1.96 and OR = 1.73, 95%CI = 1.54–1.93, respectively). Participants infected during the Alpha and Delta-dominant periods were more likely to report neurocognitive, respiratory, and anosmia/dysgeusia symptoms of PCC.
Conclusions: People infected during the Alpha- and Delta-dominant periods had a higher probability of having PCC three months after infection than those infected during the Omicron-dominant period. The lower probability of PCC with the Omicron variant must also be interpreted in absolute figures. Indeed, the number of infections with the Omicron variant being higher than with the Alpha and Delta variants, it is possible that the overall prevalence of PCC in the population increases, even if the probability of having a PCC decreases.
Video Counseling and a Safety Planning App to Support Pregnant Women Exposed to Intimate Partner Violence in Denmark and Spain During COVID-19
Authors: Andreasen Karen , Linde Ditte S , Zapata-Calvente Antonella Ludmilla , Martín-de-las-Heras Stella , Megías Jesús López , Rasch Vibeke 
Published: April 2023

Background
Isolation was a consequence of the COVID-19 lockdowns, which led to increased incidence of intimate partner violence (IPV). During antenatal care, it is possible to screen pregnant women for IPV; this offers a unique opportunity for early intervention. During the pandemic, we designed and implemented a digital IPV intervention tailored to pregnant women in Denmark and Spain.

Objective
This study aimed to identify pregnant women exposed to IPV through digital screening and offer video counseling as well as a safety planning app to those who screened positive.

Methods
Pregnant women attending antenatal care were screened for IPV through a digital questionnaire. Women who screened positive were offered 6 video consultations with an IPV counselor and provided with access to a dedicated safety planning app. In-depth interviews inspired by the Model for Assessment of Telemedicine Applications were conducted with counselors and participants. The intervention was cocreated with women who have been exposed to IPV, nongovernmental organizations, and health care providers.

Results
Despite the COVID-19 shutdowns, more than 15,000 pregnant women have been screened for IPV since January 2021. Qualitative interviews with women who completed the intervention as well as IPV counselors show broad acceptance of the video counseling, particularly the ability to participate from a safe environment and talk to a stranger about sensitive and stigmatizing issues. Preliminary findings show that both women and counselors find the video counseling highly supportive, empowering, and aligned with the needs for safety. The majority indicate improved well-being after the intervention, and neither counselors nor participants see video counseling as a barrier toward talking about sensitive topics such as IPV. The safety app was not perceived as effective.

Conclusions
The preliminary results show that video counseling conducted during the COVID-19 pandemic supports pregnant women exposed to IPV and is highly feasible and accepted in both Denmark and Spain.

Conflicts of Interest
None declared.