bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens
Authors: van der Wal Fimme J. , Achterberg René P. , van der Goot Jeanet A. , Dinkla Annemieke , Bossers-de Vries Ruth , van Solt-Smits Conny , Bossers Alex , Heijne Marloes 
Published: April 2023

AbstractThe available differentiating tests for Chlamydia are based on detection of genetic material and only give information about the actual infection status, but reveal nothing of past infections. As the use of serological methods increases the window of detection, the goal of this study was to investigate if it is possible to develop a differentiating serological test for antibodies against Chlamydia species in chicken sera. Focus was on C. psittaci, C. gallinacea, and two closely related species, i.e. C. abortus and C. avium. To enable differentiating serology, a bead-based Luminex suspension array was constructed, using peptides as antigens, derived from known immunoreactive Chlamydia proteins. For the majority of these peptides, species-specific seroreactivity in mammalian sera has been reported in literature. The suspension array correctly identified antibodies against various Chlamydia species in sera from experimentally infected mice, and was also able to differentiate between antibodies against C. psittaci and C. gallinacea in sera from experimentally infected chickens. In field sera, signals were difficult to interpret as insufficient sera from experimentally infected chickens were available for evaluating the seroreactivity of all peptides. Nevertheless, results of the suspension array with field sera are supported by published data on the occurrence of C. gallinacea in Dutch layers, thereby demonstrating the proof of concept of multiplex serology for Chlamydial species in poultry.
RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells
Authors: Lai Fengling , Wang Haoyu , Zhao Xinyue , Yang Kangning , Cai Le , Hu Mengxin , Lin Lan , Xia Xizhong , Li Wei , Cheng Hanhua , Zhou Rongjia 
Published: April 2023

Abstract
Background
Spermatogenesis depends on the supporting of the Sertoli cells and their communications with germ cells. However, the regulation of crosstalk between the Sertoli cells and germ cells remains unclear.

Results
In this report, we used conditional knockout technology to generate the Sertoli cells-specific knockout of Rnf20 in mice. The Amh-Rnf20−/− male mice were infertile owing to spermatogenic failure that mimic the Sertoli cell-only syndrome (SCOS) in humans. Knockout of Rnf20 resulted in the H2BK120ub loss in the Sertoli cells and impaired the transcription elongation of the Cldn11, a gene encoding a component of tight junction. Notably, RNF20 deficiency disrupted the cell adhesion, caused disorganization of the seminiferous tubules, and led to the apoptotic cell death of both spermatogonia and spermatocytes in the seminiferous tubules.

Conclusions
This study describes a Rnf20 knockout mouse model that recapitulates the Sertoli cell-only syndrome in humans and demonstrates that RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells.
Intravenous vitamin C monotherapy in critically ill patients: a systematic review and meta-analysis of randomized controlled trials with trial sequential analysis
Authors: Lee Zheng-Yii , Ortiz-Reyes Luis , Lew Charles Chin Han , Hasan M. Shahnaz , Ke Lu , Patel Jayshil J. , Stoppe Christian , Heyland Daren K. 
Published: April 2023

Abstract
Background
A recent landmark randomized controlled trial (RCT) in septic patients demonstrated an increased risk of death and persistent organ dysfunction with intravenous Vitamin C (IVVC) monotherapy, which represents a disparate result from previous systematic reviews and meta-analyses (SRMA). We performed an updated SRMA of IVVC monotherapy to summarize and explore heterogeneity across current trials and conduct trial sequential analysis (TSA) to guard against type-I or type-II statistical errors.

Methods
RCTs evaluating IVVC in adult critically ill patients were included. Four databases were searched from inception to 22 June 2022 without language restrictions. The primary outcome was overall mortality. Random effect meta-analysis was performed to estimate the pooled risk ratio. TSA for mortality was performed using the DerSimonian–Laird random effect model, alpha 5%, beta 10%, and relative risk reduction (RRR) of 30%, 25%, and 20%.

Results
We included 16 RCTs (n = 2130). IVVC monotherapy is associated with significant reduction in overall mortality [risk ratio (RR) 0.73, 95% confidence interval (CI) 0.60–0.89; p = 0.002; I2 = 42%]. This finding is supported by TSA using RRR of 30% and 25%, and sensitivity analysis using fixed-effect meta-analysis. However, the certainty of our mortality finding was rated low using GRADE due to the serious risk of bias and inconsistency. In a priori subgroup analyses, we found no differences between single vs multicenter, higher (≥ 10,000 mg/day) vs lower dose and sepsis vs non-sepsis trials. Post-hoc, we found no differences in subgroup analysis of earlier (< 24 h) vs delayed treatment, longer (> 4 days) vs shorter treatment duration, and low vs other risk of bias studies. IVVC may have the greatest benefit in trials that enrolled patients above (i.e., > 37.5%; RR 0.65, 95% CI 0.54–0.79) vs below (i.e., ≤ 37.5%; RR 0.89, 95% CI 0.68–1.16) median control group mortality (test for subgroup differences: p = 0.06), and TSA supported this.

Conclusions
IVVC monotherapy may be associated with mortality benefits in critically ill patients, particularly in patients with a high risk of dying. Given the low certainty of evidence, this potentially life-saving therapy warrants further studies to identify the optimal timing, dosage, treatment duration, and patient population that will benefit most from IVVC monotherapy.
PROSPERO Registration ID: CRD42022323880. Registered 7th May 2022.
Multiple-site decontamination to prevent acquired infection in patients with veno-venous ECMO support
Authors: Massart Nicolas , Camus Christophe , Nesseler Nicolas , Fillâtre Pierre , Flecher Erwan , Mansour Alexandre , Verhoye Jean-Philippe , Le Fevre Lucie , Luyt Charles-Edouard 
Published: April 2023

Abstract
Background
Acute distress respiratory syndrome (ARDS) patients with veno-venous extra corporeal membrane oxygenation (ECMO) support are particularly exposed to ECMO-associated infection (ECMO-AI). Unfortunately, data regarding AI prophylaxis in this setting are lacking. Selective decontamination regimens decrease AI incidence, including ventilator-associated pneumonia (VAP) and bloodstream infection (BSI) in critically ill patients. We hypothesized that a multiple-site decontamination (MSD) regimen is associated with a reduction in the incidence of AI among VV-ECMO patients.

Methods
We conducted a retrospective observational study in three French ECMO referral centers from January 2010 to December 2021. All adult patients (> 18 years old) who received VV-ECMO support for ARDS were eligible. In addition to standard care (SC), 2 ICUs used MSD, which consists of the administration of topical antibiotics four times daily in the oropharynx and the gastric tube, once daily chlorhexidine body-wash and a 5-day nasal mupirocin course. AIs were compared between the 2 ICUs using MSD (MSD group) and the last ICU using SC.

Results
They were 241 patients available for the study. Sixty-nine were admitted in an ICU that applied MSD while the 172 others received standard care and constituted the SC group.
There were 19 ECMO-AIs (12 VAP, 7 BSI) in the MSD group (1162 ECMO-days) compared to 143 AIs (104 VAP, 39 BSI) in the SC group (2376 ECMO-days), (p < 0.05 for all infection site). In a Poisson regression model, MSD was independently associated with a lower incidence of ECMO-AI (IRR = 0.42, 95% CI [0.23–0.60] p < 0.001). There were 30 multidrug resistant microorganisms (MDRO) acquisition in the SC group as compared with two in the MSD group (IRR = 0.13, 95% CI [0.03–0.56] p = 0.001). Mortality in ICU was similar in both groups (43% in the SC group vs 45% in the MSD group p = 0.90). Results were similar after propensity-score matching.

Conclusion
In this cohort of patients from different hospitals, MSD appeared to be safe in ECMO patients and may be associated with improved outcomes including lower ECMO-AI and MDRO acquisition incidences. Since residual confounders may persist, these promising results deserve confirmation by randomized controlled trials.
Even symmetric chaotic and skewed maps as a technique in video encryption
Authors: El-den B. M. , Raslan Walid A. , Abdullah Ahmed A. 
Published: April 2023

AbstractThe massive growth and use of digital multimedia through computer networks, including video and images, have increased the demand for protecting this digital data. To secure digital video, video encryption is frequently utilized. In this paper, a brand-new video scrambling technique based on two chaotic linearly symmetric maps and one chaotic tent map that has been twisted is suggested. The permutation procedure moves every frame pixel's position using a P-box created by permuting a linearly symmetric chaotic sequence. The diffusion technique employs both linearly symmetric chaos maps and distorted tent maps to create key streams. The keystream closely resembles simple frames because the pixels in the permuted frame indicate which of the two even symmetric chaos maps is replicated each time for the following byte. The information entropy, histogram, neighboring pixel correlation and sensitivity analysis, number of pixels changing regions (NPCR), and unified mean change intensity are used to thoroughly evaluate the recommended method's capacity to improve performance and security (UACI). Comparatively to other methods, the suggested algorithm is resistant to clipping, salt and pepper noise, speckle noise rotation assaults, and clipping. This positive outcome indicates that the plan can be successfully implemented for secure video communication applications.
Three-stage training and orthogonality regularization for spoken language recognition
Authors: Li Zimu , Xu Yanyan , Ke Dengfeng , Su Kaile 
Published: April 2023

AbstractSpoken language recognition has made significant progress in recent years, for which automatic speech recognition has been used as a parallel branch to extract phonetic features. However, there is still a lack of a better training strategy for such architectures of two individual branches. In this paper, we analyze the mostly used two-stage training strategies and reveal a trade-off between the recognition accuracy and the generalization ability. Based on the analysis, we propose a three-stage training strategy and an orthogonality regularization method. The former adds a multi-task learning stage to the traditional two-stage training strategy to extract hybrid-level and noiseless features, which can improve the recognition accuracy on the basis of maintaining the generalization ability, while the latter constrains the orthogonality of base vectors and introduces prior knowledge to improve the recognition accuracy. Experiments on the Oriental Language Recognition (OLR) dataset indicate that these two proposed methods can improve both the language recognition accuracy and the generalization ability, especially in complex challenge tasks, such as cross-channel or noisy conditions. Also, our model, which combines these two proposed methods, performs better than the top three teams in the OLR20 challenge.
FitDepth: fast and lite 16-bit depth image compression algorithm
Authors: D’Amato Juan P. 
Published: April 2023

AbstractThis article presents a fast parallel lossless technique and a lossy image compression technique for 16-bit single-channel images. Nowadays, such techniques are “a must” in robotics and other areas where several depth cameras are used. Since many of these algorithms need to be run in low-profile hardware, as embedded systems, they should be very fast and customizable. The proposal is based on the consideration of depth images as surfaces, so the idea is to split the image into a set of polynomial functions that each describes a part of the surface. The developed algorithm herein proposed can achieve a similar—or better—compression rate and especially higher speed rates than the existing techniques. It also has the potential of being fully parallelizable and to run on several cores. This feature, compared to other approaches, makes it useful for handling and streaming multiple cameras simultaneously. The algorithm is assessed in different situations and hardware. Its implementation is rather simple and is carried out with LIDAR captured images. Therefore, this work is accompanied by an open implementation in C++.
Influence of Fe2O3 Nanoparticles on the Characteristics of Waste Marble Powder Mixed Cement Mortars
Authors: Jose Prakash Arul , Alex Alexander Gladwin , Gebrehiwet Tsegay , Murugan Srinivasan 
Published: April 2023

AbstractEco-friendly and durability of material has been the focuses of different researches; however, limited are considered both the nanomaterial and byproduct. Therefore, the aimed of this study is to investigate the influence of nano-Fe2O3 (NF) on various properties of cement mortar with marble powder (MP) as supplementary cementitious material (SCM). Fresh and hardened properties of each mix were examined. The microstructure of hydrated blended cement mortars was evaluated by scanning electron microscopy and X-ray energy-dispersive techniques. The effect was evaluated using flow table, compressive, split tensile, and durability tests. In general, the MP dosage mixes showed increase in water content due to high fineness and NF increased the workability and rate of hydration. The refined pores and enhanced C–S–H gel formation showing better strength with NF than MP. The mechanical properties result compared to control specimens showed that (MP + NF > NF > MP > Control). The optimal value MP and NF in cement mortar's strength and durability is 10% MP and 1% NA. The findings showed that the use of MP and NF in concrete technology as partial replacement of cement is effective and enhance the properties of cementitious composites.
The impact of the COVID-19 pandemic on psychiatric emergency consultations in adolescents
Authors: So Pety , Wierdsma André I. , Mulder Cornelis L. , Vermeiren Robert R. J. M. 
Published: April 2023

Abstract
Background
There is growing evidence that the COVID-19 pandemic, and its associated social distancing measures, affect adolescents’ mental health. We wanted to examine whether and how the number and characteristics of adolescents’ psychiatric emergency presentations have changed throughout the pandemic.

Methods
We extracted data from the records of 977 psychiatric emergency consultations of adolescents aged 12- 19 who had been referred to the mobile psychiatric emergency services in Rotterdam, the Netherlands between January 1st 2018 and January1st 2022. Demographic, contextual, and clinical characteristics were recorded. Time-series-analyses were performed using quasi-Poisson Generalized Linear Model to examine the effect of the first and second COVID-19 lockdown on the number of psychiatric emergency consultations, and to explore differences between boys and girls and internalizing versus externalizing problems.

Results
The number of psychiatric emergency consultations regarding adolescents increased over time: from about 13 per month in 2018 to about 29 per month in 2021. During the COVID-19 pandemic, the increase was tempered. In the second wave a pronounced increase of psychiatric emergencies among adolescents with internalizing problems but not with externalizing problems was found.

Conclusion
Despite the reported increase of mental health problems in adolescents during the COVID-19 pandemic, we did find a smaller increase in psychiatric emergency consultations in this group then would be expected considering the overall trend. Besides changes in help-seeking and access to care, a possible explanation may be that a calmer, more orderly existence, or more parental supervision led to less psychiatric emergency situations in this age group. In the second wave the number of emergency consultations increased especially among girls with internalizing problems. While there has been a particular fall in emergency referrals of adolescents with externalizing problems since the start of the pandemic it is still too early to know whether this is a structural phenomenon. It would be important to elucidate whether the changes in emergency referrals reflect a true change in prevalence of urgent internalizing and externalizing problems in adolescents during the pandemic or a problem related to access to care.
Basic psychological needs satisfaction of stroke patients: a qualitative study
Authors: Lu Huiqi , Tan Xiyi , Wang Xiangmin , Lin Qinger , Huang Simin , Li Jinjun , Zhou Hongzhen 
Published: April 2023

Abstract
Background
Previous studies have shown that the satisfaction of basic psychological needs is related to psychological well-being. Improving satisfaction will increase personal well-being, promote positive health outcomes, and improve disease recovery. However, no research has focused on the basic psychological needs of stroke patients. Therefore, this study aims to determine the basic psychological needs experience, satisfaction, and its influencing factors of stroke patients.

Methods
12 males and 6 females in the non-acute phase with stroke were recruited in the Department of Neurology, Nanfang Hospital. The individual, semi-structured interviews were conducted in a separate room. The data were imported to Nvivo 12 and analyzed using the directed content analysis approach.

Results
Three main themes consisting of 9 sub-themes were derived from the analysis. These three main themes focused on the needs for autonomy, competence, and relatedness of stroke patients.

Conclusion
Participants have different degrees of satisfaction of their basic psychological needs, which may be related to their family environment, work environment, stroke symptoms, or other factors. Stroke symptoms can significantly reduce the patients’ needs for autonomy and competence. However, the stroke seems to increase the patients’ satisfaction of the need for relatedness.
Is the meaning of subjective well-being similar in Latin American countries? A cross-cultural measurement invariance study of the WHO-5 well-being index during the COVID-19 pandemic
Authors: Caycho-Rodríguez Tomás , Vilca Lindsey W. , Valencia Pablo D. , Carbajal-León Carlos , Reyes-Bossio Mario , White Michel , Rojas-Jara Claudio , Polanco-Carrasco Roberto , Gallegos Miguel , Cervigni Mauricio , Martino Pablo , Palacios Diego Alejandro , Moreta-Herrera Rodrigo , Samaniego-Pinho Antonio , Lobos-Rivera Marlon Elías , Buschiazzo Figares Andrés , Puerta-Cortés Diana Ximena , Corrales-Reyes Ibraín Enrique , Calderón Raymundo , Franco Ferrari Ilka , Flores-Mendoza Carmen 
Published: April 2023

Abstract
Background
There is an urgent need to assess changes in well-being on a multinational scale during the COVID-19 pandemic, thus culturally valid scales must be available.

Methods
With this in mind, this study examined the invariance of the WHO well-being index (WHO-5) among a sample of 5183 people from 12 Latin Americans countries (Argentina, Bolivia, Chile, Colombia, Cuba, Ecuador, El Salvador, Guatemala, Mexico, Paraguay, Peru, and Uruguay).

Results
The results of the present study indicate that the WHO-5 is strictly invariant across samples from different Latin American countries. Furthermore, the results of the IRT analysis indicate that all items of the WHO-5 were highly discriminative and that the difficulty required to respond to each of the five items is ascending. Additionally, the results indicated the presence of moderate and small size differences in subjective well-being among most countries.

Conclusion
The WHO-5 is useful for assessing subjective well-being in 12 Latin American countries during the COVID-19 pandemic, since the differences between scores can be attributed to differences in well-being and not in other characteristics of the scale.
Digitalisation in accounting: a systematic literature review of activities and implications for competences
Authors: Pargmann Julia , Riebenbauer Elisabeth , Flick-Holtsch Doreen , Berding Florian 
Published: April 2023

AbstractThe digitalisation of processes is a current topic in accounting. New technologies can change activities which in turn may require different skills from accounting graduates. This paper aims to shed light on the changes that digitalisation brings about in various areas of accounting by assessing the types of activities (non-routine and routine) and corresponding competences in the context of progressing stages of digitalisation. In addition, it is analysed how different technologies are used in these activities and where their execution is placed within the supply chain. The systematic literature review shows a lack of expertise in the field of digitalisation that enables graduates and employees to successfully manage respective processes in the workplace. While routine activities are continuously being automated or digitalised, non-routine activities and the corresponding skills have a similarly increasing importance for employees in accounting as the acquisition of general digital competences.
Advancing learning-oriented assessment (LOA): mapping the role of self-assessment, academic resilience, academic motivation in students’ test-taking skills, and test anxiety management in Telegram-assisted-language learning
Authors: Çakmak Fidel , Ismail Sayed M. , Karami Samaneh 
Published: April 2023

AbstractSome impediments in language learning may have a detrimental impact on learners’ actual performance on the test and lead to anxiety and demotivation. Language achievement is influenced by self-assessment (SA), academic resilience (AR), academic motivation (AM), and test-taking skills (T-TS) among other factors. Considering the relevance of these factors in language achievement, the current investigation aims to delve into the probable interactions of SA, AR, AM, T-TS, and test anxiety (TA) management among English as a foreign language (EFL) learners. A model was devised and evaluated using confirmatory factor analysis (CFA) and structural equation modeling (SEM) to achieve this objective. This research collected 512 by distributing online questionnaires to fifteen approved private institutions which applied Telegram-based language learning. The study findings reflected that SA, AR, and AM could predict EFL learners’ T-TS. It was also confirmed that SA, AR, and AM modulated EFL learners’ TA. The implications of the study are presented and accompanied by some future research proposals as well as instructional consequences.