bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Analysis of Acquired Resistance to Bortezomib in Prostate Cancer Cells Using Proteomic and Bioinformatic Tools
Authors: Seker Semih , Sahin Betul , Yerlikaya Azmi 
Published: January 2025

ABSTRACTChemotherapy is a potent tool against cancer, but drug resistance remains a major obstacle. To combat this, understanding the molecular mechanisms behind resistance in cancer cells and the protein expression changes driving these mechanisms is crucial. Targeting the Ubiquitin‐Proteasome System (UPS) has proven effective in treating multiple myeloma and shows promise for solid tumours. Despite initial success with the proteasome inhibitor bortezomib, acquired resistance soon after treatment poses a significant challenge to its efficacy. In this study, we explored proteins potentially involved in acquired resistance to bortezomib using label‐free nLC–MS/MS proteomic analysis. The investigation revealed 299 proteins with notable differences in expression levels in the bortezomib‐resistant PC3 prostate cancer cell line. Using bioinformatics tools, we illustrated the top 10 gene ontology (GO) processes [e.g., translational initiation (p = 5.964E‐10), CRD‐mediated mRNA stabilisation (p = 1.636E‐5), and hydrogen ion transmembrane transport (p = 6.46E‐5)] and the top 20 KEGG [e.g., metabolic pathways (p = 7.601E‐13), biosynthesis of amino acids (p = 3.834E‐12), and chemical carcinogenesis—reactive oxygen species (p = 1.891E‐4)] and REACTOME [e.g., metabolism (p = 4.182E‐21), translation (p = 9.484E‐18), and Nonsense‐Mediated Decay (NMD) (p = 1.829E‐8)] pathways in the PC3‐resistant cells. We further refined our results by comparing them with globally validated TCGA datasets. We correlated the 299 proteins identified through proteomic analysis with tumour aggressiveness and resistance by comparing them with the TCGA nodal metastasis N0 vs. N1 datasets using the UALCAN portal and identified 37 proteins consistent with our results. We believe that a combination of bortezomib with chemotherapeutics targeting these proteins could be effective in overcoming the resistance developed against bortezomib.
Therapeutic Ultrasound Modulates Cell Proliferation and Proinflammatory Cytokine Levels in Osteoarthritic Chondrocytes
Authors: Yilmaz Ahmet Çagdas , Toktas Hasan , Celik Sefa , Sen Serkan 
Published: January 2025

ABSTRACTThe development and progression of osteoarthritis (OA) are believed to involve inflammation. This study aimed to investigate the effects of applying therapeutic ultrasound (US) to human osteoarthritic chondrocytes in continuous and pulsed modes on cell proliferation and proinflammatory cytokine levels. Human osteoarthritic chondrocytes (HC‐OA 402OA‐05a) were proliferated in appropriate media and then seeded into culture plates. The plates were grouped and exposed to underwater continuous, pulsed and control US at 0.1 W/cm2 and 1 MHz for 10 min daily for 10 days. Cell viability/proliferation was assessed using the MTT assay, total protein was measured by ELISA and cytokine levels per protein were determined. Cells were photographed using microscopic analysis. Both continuous and pulsed US groups showed a significant increase in viability compared to the control group. No significant difference was found between the continuous and pulsed US groups for IL‐1β, TNF‐α and IL‐6 levels. Both groups showed significant cytokine reduction compared to the control group. For IL‐17 and IL‐32 levels, both US groups had reduced cytokine levels compared to the control group, but the results were not significant. Underwater US at 0.1 W/cm2 and 1 MHz stimulated cell proliferation and reduced proinflammatory cytokine levels in osteoarthritic chondrocyte cell cultures. This study extensively focused on proinflammatory IL levels, and the meaningful results may inspire future in vivo/in vitro studies. While adapting in vitro data to in vivo conditions poses challenges, our results could guide future in vivo studies and clinical applications.
Serum Nutrients, Periodontitis and Biological Ageing
Authors: Nguyen L. M. , Tran A. V. , Kincheloe J. P. , Ebersole J. E. 
Published: January 2025

ABSTRACTIntroductionTelomeres are nucleotide sequences found at the end of chromosomes, and their shortening is associated with chronological and biological ageing, oxidative stress and malnutrition. Shorter telomeres have been shown to be associated with periodontitis. Dietary nutrients are also influential factors in the aetiology and progression of periodontitis and other chronic inflammatory diseases. We tested the hypothesis that blood serum levels of folate, vitamin D, vitamin E, cis‐β‐carotene and/or β‐cryptoxanthin are associated with telomere lengths and periodontitis and that these relationships are modified by factors that also affect periodontitis (e.g., age, sex, smoking, race/ethnicity).MethodsLaboratory and demographic data collected by the NHANES 1999–2002 survey of 10,793 study participants were analysed. The data included age (binned every 10 years starting at age 30), race/ethnicity (White, Black, Hispanic), sex (male, female), periodontitis (none/mild, moderate/severe), vitamin levels (low, medium, high), telomere lengths (base pairs) and smoking (yes, no). Statistical analyses were performed with ANOVA tests.ResultsPeriodontitis was significantly associated with vitamin D, vitamin E and cis‐β‐carotene. Telomere length was significantly associated with vitamin E and cis‐β‐carotene. There were statistically significant interactions between the following vitamins and covariates on telomere length: folate and sex, vitamin D and periodontitis, vitamin D and race/ethnicity and vitamin E and sex.ConclusionsCertain blood nutrients may disrupt biological ageing, with periodontitis as a co‐morbidity. Some of these relationships are modified by demographic variables such as sex and race/ethnicity.
Advancing surgical education: An evaluation of laparoscopic simulation training for medical students
Authors: Zhang Wenbin , Qu Lijun , Zilundu Prince Last Mudenda , Xu Yuanyuan , Chen Meiying , Yu Guangyin , Zheng Xuefeng , Guo Guoqing , Zhang Jifeng 
Published: January 2025

AbstractObjectiveTo examine the medical students' awareness of laparoscopic surgery as well as assess the perceived importance of laparoscopic simulation training, and its impact on students' confidence, career aspirations, proficiency, spatial skills, and physical tolerance.DesignDescriptive and comparative study using pre‐ and post‐training assessments.SettingSimulation training sessions centred on laparoscopic surgery techniques.ParticipantsMedical students in year three (n = 49) participated in laparoscopic simulation training sessions.ResultsBefore the simulation training, 85.7% of students were unaware of laparoscopy, with females being less familiar than males (96.3% vs. 72.8%). 85.7% believed mastering laparoscopy was crucial before training, and 81.6% believed simulation training could enhance surgical skills. A disparity existed in this belief between the sexes, with 91% of males and 74.1% of females seeing the value in such training for skill improvement. Pretraining, males (54.5%) were more confident than females (26%) in mastering laparoscopy. Post‐training, confidence increased for both groups, and the percentage of students wishing to pursue a surgical career also rose from 50% to 72.7%. Despite initial disparities in the time spent on training modules between males and females, both groups achieved similar proficiency levels by the end of the training. Although females initially lagged in spatial awareness and skills, post‐training results showed significant improvement, matching their male counterparts. 26.5% of students experienced physical fatigue post‐training, with a higher percentage of females (33.3%) reporting fatigue than males (18.2%). Regarding concentration during simulations, 81.8% of males could maintain focus compared to 48.2% of females.ConclusionsLaparoscopic simulation training effectively improved the understanding, confidence, and surgical skills of medical students, with evident benefits in shaping their career aspirations. While both genders exhibited significant gains, female students faced challenges in terms of physical tolerance and initial spatial awareness. However, their post‐training achievements mirrored those of their male peers, highlighting the effectiveness and importance of such simulation training programmes.
Accuracy of Guided Dual Technique in Esthetic Crown Lengthening: A Prospective Case‐Series Study
Authors: Enfedaque‐Prat Meritxell , González‐Barnadas Albert , Jorba‐García Adrià , Vilarrasa Javi , Toledano‐Serrabona Jorge , Figueiredo Rui , Valmaseda‐Castellón Eduard , Camps‐Font Octavi 
Published: January 2025

ABSTRACTObjectiveThis study aimed to evaluate the efficacy and safety of a digitally guided dual technique during esthetic crown lengthening surgery. In addition, patient satisfaction and patient‐reported outcomes were assessed.Materials and MethodsA prospective case series study was conducted. Cone‐beam computed tomography and intraoral scans were used to design surgical guides, which were manufactured via 3D printing. The primary outcome was surgical accuracy, assessed by measuring the distance between the planned and final gingival margin positions using overlapping intraoral scans. Secondary outcomes included clinical crown length, gingival margin stability, pain, and patient satisfaction. Statistical analyses were performed using multilevel linear regression models, with significance set at p < 0.05.ResultsTen participants (87 teeth) were treated without complications. The mean duration of surgery was 66.5 min. The overall absolute deviation was 0.56 mm (95% CI: 0.48 to 0.65) at 6 months postoperatively. Clinical crown length increased significantly from baseline to the end of surgery (p < 0.001), with minimal reduction at 6 months (p = 0.479). Patient‐reported outcomes indicated mild postoperative pain and high satisfaction with esthetic results.ConclusionsThe digitally guided dual technique for esthetic crown lengthening surgery is safe and effective, providing highly accurate outcomes. The technique also results in excellent patient satisfaction.Clinical SignificanceThe use of digitally guided dual techniques for ACL surgery enhances precision and safety, leading to highly accurate outcomes and improved patient satisfaction. This approach could be beneficial in clinical settings to ensure better esthetic and functional results.
Use of Ozone to Reduce the Proportion of Mesophilic and Psychrotrophic Bacteria Among Aquatic Organisms: A Systematic Review and Meta‐Analysis
Authors: de Arruda Luciano Carlos , Porto Yuri Duarte , Vieira Bruno Serpa , dos Santos Fogaça Fabiola Helena , Castro Vinícius Silva , de Souza Figueiredo Eduardo Eustáquio , Savay‐da‐Silva Luciana Kimie 
Published: January 2025

ABSTRACTMesophilic and psychrotrophic bacteria are agents of spoilage in aquatic organisms. Aquaculture aims to provide safe and nutritious foods from microbiological, physico–chemical, and sensory perspectives. Ozone (O3) is a gas used as an efficient antimicrobial agent for inactivating microorganisms in various foods. Therefore, this study aimed to determine whether O3 is effective in reducing or eliminating mesophilic and psychrotrophic bacteria in fish and seafood. To this end, a systematic review was performed to evaluate the data obtained from published primary studies. Articles were retrieved from the PubMed, Web of Science, Scopus and SciELO databases, and papers published between 2002 and 2024 were considered. A total of 1415 studies were identified, 11 of which met all eligibility criteria and were included in the meta‐analysis. The results indicated that O3 reduced mesophilic and psychrotrophic bacteria counts by 0.31 log CFU g−1 in fish and seafood. Significant heterogeneity persisted in the data set even after separation into subgroups, indicating that the studies were significantly diverse in terms of methodology. Therefore, the data obtained in this meta‐analysis indicates low inactivation of the microorganisms studied using O3. However, more research into the O3 treatment of aquatic organisms should be encouraged, since the included studies had methodological variations in the forms of O3 used, and investigated species, since all species have their own specificities.
South African Dietitians' Knowledge and Perceptions of Food‐Drug Interactions and Factors Affecting It
Authors: Megaw Christie , Olivier Natascha , Cordier Werner 
Published: January 2025

ABSTRACTBackgroundDietitians ensure that patients receive tailored medical nutrition therapy to integrate with pharmacotherapy safely. Dietitians require a pharmacological understanding to prevent detrimental food‐drug interactions (FDIs). The study investigated dietitians' knowledge of FDIs and their information sourcing.MethodsA cross‐sectional online survey was conducted among registered South African dietitians to assess their knowledge of FDIs, the impact of food timing on drugs, and their sources of FDI information. The questionnaire included demographics, a 12‐question knowledge assessment, and a qualitative section on information sourcing. Data from 70 valid responses, collected between 2 August and 19 September 2022, were analysed statistically using analysis of variance and chi‐square tests to determine whether associations between knowledge scores and demographic factors were present.Results and DiscussionOut of 70 responses, most participants were female (97.1%) and 47.1% had over 10 years of experience. The participants primarily worked in the areas of dietetics related to chronic and lifestyle‐related disorders (75.7%) across various work settings, including in‐patient care (32.8%), out‐patient care (41.4%), and multi‐disciplinary team environments (31.4%). Although not generalisable due to the low response rate (70 out of the 304 required responses for a 5% margin of error), knowledge deficiencies were observed. A cumulative mean knowledge score of 38.3% was observed, with gaps identified for fundamental FDIs. Drug package inserts (55.7%) and clinical websites (68.6%) were primarily used to source information regarding FDIs; however, the former did not always provide sufficient information. Participants proposed that knowledge deficiencies could be overcome with further education, and the development and/or use of mobile applications or summarisations that elaborate on FDIs.ConclusionKnowledge gaps and uncertainties were identified regarding fundamental FDIs; however, further research is needed to pinpoint the specific sources of these deficiencies and the factors influencing them. To improve dietitians' knowledge of FDIs and ensure alignment with their scope and standard of practice, undergraduate curricula should be bolstered and benchmarked to national needs to facilitate graduate development, and additional learning opportunities provided, such as webinars and continuing professional development (CPD), to allow for continuous education for practicing dietitians.
Minority stress and structural stigma predict well‐being in European LGBTQ+ parents
Authors: Siegel Magdalena , Steyrl David , Goldberg Abbie E. , Nicholson Andrew A. , Zemp Martina 
Published: January 2025

AbstractObjectiveThis study tested whether exposure to minority stress and structural stigma across multiple levels of the family system were associated with two indicators of well‐being (life satisfaction, depressive symptoms) in LGBTQ+ parents across 19 European countries.BackgroundMinority stress (i.e., identity‐based stress resulting from systemic oppression) and structural stigma (i.e., hostile legal environments, prejudicial social attitudes) are heterogeneous, yet well‐documented risk factors of reduced well‐being within LGBTQ+ populations. However, a comprehensive assessment stratifying both concepts across multiple levels of the family system (i.e., the individual, couple, and family level) is lacking for LGBTQ+ parents.MethodUsing data from the EU LGBTI Survey 2019, a sample of 3808 LGBTQ+ parents from 19 European countries was analyzed. Associations between self‐reported minority stress indicators, objective structural stigma indicators, sociodemographic predictors, and well‐being were tested using non‐linear, machine learning‐based techniques (gradient boosted decision tree models).ResultsSupporting preregistered hypotheses, exposure to individual‐level minority stress and individual‐ and family‐level structural stigma predicted life satisfaction and depressive symptoms. Couple‐level minority stress predicted life satisfaction, but not depressive symptoms, and family‐level minority stress predicted neither. Trans parents and those facing economic burdens were particularly vulnerable to low well‐being.ConclusionsExposure to minority stress and structural stigma, particularly within highly stigmatizing regions, are risk factors for LGBTQ+ parents' well‐being. Future research should examine the role of family‐level minority stress using validated measures.
Fusarium spp. Associated With Leaf Blight of Hosta (Hosta plantaginea L.) in China
Authors: Jiao Chenyang , Zhang Li , Xu Xi , Yang Xilang , Guo Lifeng , Wang Xiangjing , Xiang Wensheng , Zhao Junwei 
Published: January 2025

ABSTRACTHosta (Hosta plantaginea L.) is a perennial herbaceous plant belonging to the family Liliaceae. It is widely cultivated for its decorative foliage and dainty, colourful flowers. Fusarium is a globally prevalent plant pathogen. During 2020–2021, 116 Fusarium isolates were isolated from 300 hosta leaf blight samples collected from eight cities in China, and 65 representative isolates we selected for further study. The 65 isolates were identified based on the concatenated sequences of the tef1 and rpb2 genes, which grouped them into 12 Fusarium species, including F. acuminatum (27.59%), F. verticillioides (15.52%), F. oxysporum (10.34%), F. armeniacum (10.34%), F. ipomoeae (8.62%), F. proliferatum (6.9%), F. subglutinans (6.9%), F. humuli (3.45%), F. petersiae (3.45%), F. brachygibbosum (3.45%), F. graminearum (1.72%), and F. compactum (1.72%). Pathogenicity tests demonstrated that all Fusarium isolates exhibited high pathogenicity towards hosta leaves. Whereas in F. proliferatum isolates were found to be the most pathogenic. All Fusarium species obtained in this study are first reported as the causal agents of hosta leaf blight in China except F. oxysporum and F. ipomoeae. The current research offers insights into disease management, phylogenetic relationships amongst Fusarium species and the diversity of Fusarium species associated with hosta leaf blight.
Nutritional consistency of macroalgae across a sea ice cover gradient along the Western Antarctic Peninsula
Authors: Oswalt Hannah E. , Amsler Margaret O. , Amsler Charles D. , Iken Katrin , McClintock James B. , Klein Andrew G. , Galloway Aaron W. E. 
Published: January 2025

AbstractSea ice can profoundly influence photosynthetic organisms by altering subsurface irradiance, but it is susceptible to changes in the climate. The patterns and timing of sea ice cover can vary on a monthly to annual timescale in small sub‐regions of the Western Antarctic Peninsula (WAP). During the latter part of the 20th century, sea ice coverage significantly decreased in the WAP, a trend that aligns with warming in this area. Macroalgal biochemical components are impacted by light availability, often showing a close relationship between photosynthesis and biochemical compositions. We used satellite imagery of annual sea ice duration and extent as well as water turbidity during ice‐free periods to identify 14 study sites that differed dramatically in sea ice coverage but were similar in terms of turbidity along the central WAP between 68° S and 64° S. The common macroalgal species Desmarestia menziesii, Himantothallus grandifolius, Sarcopeltis antarctica, and Iridaea sp. were collected by scuba divers between 5 m and 35 m depth at each site where they occurred, for later biochemical analyses. Overall percentages of major biochemical components as well as carbon and nitrogen percentages and C:N were determined and correlated with four different sea ice indices. Surprisingly, most of the chemical components were not significantly correlated with sea ice cover. The few significant correlations varied between species and chemical components. This indicates that although patterns of sea ice coverage have major implications for macroalgal abundance, on a per‐biomass basis, sea ice coverage does not impact the nutritional contributions of macroalgae to food webs.
5‐FU Weakens Defensive Functions of the Junctional Epithelium
Authors: Aellos Fabiana , Ramos Amarissa , Civit‐Balta Alba , Grauer Joseph A. , Cuevas Pedro L. , Rao Samyak , Yuan Xue , Liu Bo , Helms Jill A. 
Published: January 2025

ABSTRACTAimTo investigate additional factors contributing to the pathophysiology of chemotherapy‐induced oral mucositis and periodontitis beyond the systemic immune suppression caused by the chemotherapeutic agent 5‐Fluorouracil (5‐FU).Methods5‐Fluorouracil was topically delivered to the non‐keratinized, rapidly proliferating junctional epithelium (JE) surrounding the dentition, and acts as an immunologic and functional barrier to bacterial ingression. Various techniques, including EdU incorporation, quantitative immunohistochemistry (qIHC), histology, enzymatic activity assays, and micro‐computed tomographic (μCT) imaging, were employed to analyze the JE at multiple time points following topical 5‐FU treatment. Systemic 5‐FU delivery was used for comparison, and all 5‐FU treated tissues were compared to vehicle‐treated controls.ResultsWe first showed that systemic 5‐FU blocked mitotic activity that rapidly led to JE atrophy. This atrophy was accompanied by suppression of the immune system. We then demonstrated that topical 5‐FU delivery effectively inhibited cell proliferation in the JE. Quantitative immunohistochemical (qIHC) analyses further demonstrated a progressive breakdown in JE barrier functions following topical 5‐FU. CBC analyses confirmed that topical 5‐FU did not alter the innate immune system but did suppress the local immune response of the JE. The longer‐term consequences of this disruption in JE barrier functions were significant alveolar bone loss and an increase in porosity. Together, these results document the essential requirement for rapid JE cell proliferation to maintain homeostasis of the periodontium.ConclusionsThe reduction of cell division in the JE due to 5‐FU treatment directly compromises both its structural integrity and immune surveillance capabilities, contributing to the destruction of periodontal hard tissues.