bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vitro Study of Fermented Complete Feed by Using Sago Residues as Main Source Diet
Authors: Samadi Samadi , Wajizah Sitti , Usman Yunasri 
Published: March 2023

Recently, fermentation of low quality feed such as agro industry by products has been widely applied to produce enriched animal feed and improve animal productivity. The purpose of this study was to evaluate in vitro digestibility of fermented complete feed by using agro-residues from sago starch processing industries as main source diet. Feed was formulated on the basis of 40% sago residue and mixed with other ingredients (rice brand, coconut meal, bread by product, soybean meal and soybean hulls) to fulfill the requirement of sheep with 16,10% of CP, 2,80 Mkal ME/kg and TDN 60,88% based on calculation. Mineral and Urea were added in the complete feed to reach mineral and CP requirements of sheep. Complete feed formulation was fermented by using 3 commercial fermentation products (Saus Burger Pakan® (SBP), Probion® and EM4®) for 21 days. Total and types of microbes from commercial fermentation products which were utilized in this study were not determined. Parameters measured in this study were pH, NH3, IVDMD (in vitro dry matter digestibility), IVOMD (in vitro organic matter digestibility) and VFA total. The model used for the statistical analysis was completely randomized design (CRD) with 4 treatments (control and 3 different commercial fermentation products) and 4 replications. The results of this study indicated that administration of different commercial fermentation products into fermented complete feed based on sago residues significantly influenced (P<0.05) on pH, NH3, VFA total, IVDMD and IVOMD. Generally, commercial fermentation products mainly SBP produced better feed quality by improving the values of pH, NH3, VFA total, IVDMD and IVOMD. However, types and total microorganisms were needed to be determined before experiment.
A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection
Authors: Mashuni Mashuni , Yanti Nur Arfa , Irnawati Irnawati , Hamid Fitri Handayani , Jahiding Muhammad 
Published: March 2023

Long-term accumulation of pesticides in the environment to human and animal health. Acetylcholinesterase (AChE) biosensors with highly sensitive potentiometer transducers based on the membranes of Ag, reduced graphene oxide (rGO), and chitosan (CS) has been successfully developed. The membrane was made with a composition of 0.5 mM AgNO3, 2.5 mg/mL rGO, and 2% (w/v) CS coated on the surface of the Au electrode. The composition of the membrane with three ratios, namely 1:1:2, 2:1:3, and 3:1:4. Then, membrane Ag/rGO/CS and the enzyme AChE were immobilized on the membrane surface. The prepared biosensor has excellent conductivity, catalytic activity, and biocompatibility attributed to the synergistic effect of Ag/rGO/CS and glutaraldehyde (GTA) as crosslinkers and providing a hydrophilic surface for AChE adhesion. The linear range in biosensors is 1 × 10-8 to 1 µg L-1 with a regression coefficient of 0.9803 for 1:1:2 membrane, 0.9836 for 2:1:3 membrane, and 0.9850 for 3:1:4 membrane. The LOD is about 1 × 10-7 µg L-1 for all membranes. In addition, the biosensor showed good sensitivity, acceptable reproducibility, and stability, having an RSD of less than 5%. This biosensor makes it possible to provide a new and promising tool for analyzing pesticides, especially organophosphates.
Recurrent Giant Cell Tumour of the Calcaneum - A Case Report
Authors: S Varun Roheet S , Prakash Siva , R Balaji , Ramesh Chepauk , Kannan Arun 
Published: March 2023

Abstract
Introduction - A 20-year-old female who presented with multiply recurrent giant cell tumour (GCT) of the left calcaneum and destruction of talus. To our knowledge, there has been no literature published on the method for reconstruction of the hind foot after complete removal of both calcaneum and talus.
Case presentation - She was managed with an en-bloc talocalcaneal resection and reconstruction with femoral head allograft. She developed soft tissue recurrence at one year and was managed with wide excision of the soft tissue component and flap coverage.
Conclusion - At 5 years follow up, she was free of recurrence and had painless ambulation.This case illustrates successful use of femoral head allograft for reconstruction of both talus and calcaneum, and management of soft tissue recurrence with wide local excision.
WITHDRAWN: Effects of AMV and WCMV combined infection on the chloroplasts ultrastructure in Nicotiana benthamiana
Authors: CHEN ying-e , LIANG Qiao-lan , WEI Lie-xin , CHENG shou-feng 
Published: March 2023

Abstract
In order to clarify the synergism pathogenic mechanism of alfalfa mosaic virus (AMV) and white clover mosaic virus (WCMV) combined infection Nicotiana benthamianaunder indoor conditions, In this experiment, the effect of AMV and WCMV combined infection on the chlorophyll content, morphology and microstructure in N. benthamiana, the relative expression of the two viruses at different infected times were observed. The results showed that AMV and WCMV combined infection the chlorophyll content was significantly lower than that in N. benthamianaalone infection and healthy; electron microscopy observed the cytoplasmic ultralesions found that two forms of viruses in the AMV and WCMV combined infection of N. benthamiana, the length of bacillus virus was 20~75 nm observed in AMV alone infection, the length of linear virus was 400~600 nm observed in WCMV alone infection; after the AMV and WCMV combined infection of N. benthamiana, chloroplasts showed cleavage, osmium granules, mitochondrial crest voids increased, mitochondrial malformations were serioused, the chloroplasts internal layer structure was loose and flocculent in N. benthamiana by AMV、WCMV alone infection; real-time quantitative detection showed that the relative expression of AMV and WCMV reached the maximum on the 21 d, which increased by 39.46% and 31.53% compared with AMV and WCMV alone infection.
Preparation of low internal resistance electrode material with multistage interconnected pores from coffee grounds
Authors: Li Bin , Li Jian , Guo Minghui 
Published: March 2023

Abstract
Using biomass waste materials to prepare electrode materials with excellent properties is an effective strategy for solving current energy and environmental problems. In this work, coffee grounds were pretreated with Co(NO3)2 and Ni(NO3)2, then KOH was used to activate the pretreated coffee grounds at a high temperature to obtain a foam-like electrode material with interconnected microporous-mesoporous-macroporous hierarchical channels. This preparation method is simple and has low energy consumption, and the resulting material has an ultra-low internal resistance of 0.31 Ω. The specific capacitance of CGC-2 is 302.65 F g− 1 at a current density of 1 A g− 1. The low internal resistance and high electrical conductivity of this activated material are attributed to the presence of Co2+ and Ni2+ during carbonization, whose catalytic effect leads to a relatively ordered lattice structure. The interconnected structure of the final product is mainly caused by the strong activation function of KOH generating many pores. The prepared material exhibits good rate performance and cycling stability, and it has a Coulombic efficiency of nearly 100%. This work provides a novel idea for using biomass materials to fabricate high-performance electrode materials for supercapacitors.
Association between cooking patterns and hyperlipidemia in Eastern China
Authors: Cui Bin , Yuan Wen Kai , Wang Linda Dong-Ling , Wang Fu Rong , Peng Jing , Ma Jian Ying , Chen Xiang , Xu Mei Yin , Ke Jun , Tian Yi 
Published: March 2023

Abstract
Background: Hyperlipidemia is a major risk factor for many diseases. Previous studies have shown that diet is closely associated with hyperlipidemia. However, the relationship between cooking methods and hyperlipidemia remains unclear. The objective of this study was to identify the major cooking patterns existing in the Eastern Chinese population and evaluate their association with the risk of hyperlipidemia.
Methods: We interviewed 4,710 residents in Eastern China regarding the consumption frequency of each cooking method when they prepare food at home or when eating out and regarding the prevalence of hyperlipidemia. Factor analysis, Chi-square test, analysis of variance, and binary logistic regression analysis were used to identify the cooking patterns and analyze the characteristics of participants’ categories of cooking patterns and the relationship between different cooking patterns and hyperlipidemia.
Results: Three major cooking patterns were identified: Traditional Chinese, Bland, and High-temperature cooking patterns. After controlling for potential confounders, participants in the highest quartile of the Bland cooking pattern had lower odds of hyperlipidemia than those in the lowest quartile. Nevertheless, no significant associations were observed between the Traditional Chinese and High-temperature cooking patterns and the risk of hyperlipidemia.
Conclusions: This study confirms the association between cooking patterns and the risk of hyperlipidemia and indicates that the Bland cooking pattern is associated with a reduced risk of hyperlipidemia.
The role of BFGF-related transcriptional regulation and alternative polyadenylation in calcification model of valve interstitial cells induced by osteoblastic medium
Authors: Zhang Jiajun , Wu Jun , Gao Yuan , Fan Xingli , Liu Xiaohong , Zhang Guanxin , Tang Yangfeng , Han Lin 
Published: March 2023

Abstract
Objective
Calcific aortic valve disease (CAVD) is a major cause of aortic stenosis resulting in angina, heart failure, and death. However, the molecular mechanism of its progression, especially the sophisticated disease-related transcriptional regulation machinery remains to be further elucidated.
Methods
This study was modeled on porcine valvular interstitial cells (PVIC). We used OIM to induce calcification and bFGF treatment to inhibit calcification. The mRNA expression profile of PVICs and their related transcriptional regulation were investigated using transcriptome sequencing. We used DaPars to further examine alternative polyadenylation (APA) among the different treatment groups.
Results
We identified global APA alteration in the OIM, OIM-bFGF, and Ctrl treatment groups. We also identified the CAT gene as one of the crucial candidate genes that may contribute to calcification inhibition through APA regulation. The alteration of both APA and protein levels of the CAT gene in the bFGF-treated group was further validated using qRT-PCR and western blotting analysis.
Conclusion
We described a global APA change in a calcification model related to CAVD. We revealed that transcriptional regulation of the CAT gene may contribute to bFGF-induced calcification inhibition.
Drug-induced bilateral ciliochoroidal detachment and uveitis in a patient with COVID-19
Authors: yao Huiping , Chen Yanwei , Zhou Yufeng , Liu Xiaoqing , Shen Xi 
Published: March 2023

Abstract
Background: To describe a case of bilateral transient myopia with shallow anterior chamber, ciliochoroidal detachment and uveitis in both eyes induced by indapamide intake following SARS-CoV-2 infection.
Case presentation: A 37-year-old man with COVID-19 was referred to our department for bilateral visual blurring. He had been treated with ibuprofen for fever and indapamide to treat the uncontrolled blood pressure. After indapamide intake for four days, he started complaining of bilateral visual blurring. On ocular examination, the uncorrected visual acuity was 20/400 OD and 20/400 OS. Slit-lamp examination revealed shallow anterior chamber. In the patient’s subsequent visit on the next day, he complained of pain and redness in both eyes from the previous night. On ocular examination, the IOP decreased significantly compared to the previous day, 11 mmHg and 12 mmHg in OD and OS respectively. Slit-lamp examination revealed conjunctival injection and inflammatory cells (2+) in the shallow anterior chamber of both eyes. Ultrasound biomicroscopy revealed ciliary body detachment, and B-scan ultrasound showed peripheral shallow choroidal detachment in both eyes. Stopping the indapamide and treatment with oral prednisolone, topical tobramycin dexamethasone and tropicamide phenylephrine eye drops resulted in rapid recovery of the signs and symptoms after 3 days.
Conclusions: Indapamide intake can induce bilateral shallow anterior chamber, ciliochoroidal detachment in both eyes, and the preexisting hyperinflammation induced by COVID-19 might increase the susceptibility; drug usage and SARS-CoV-2 infection might commonly contribute to the uveitis. Timely diagnosis and treatment can result in good prognosis.
piRNA hsa_piR_019914 promoted chondrocyte anabolic metabolism by inhibiting LDHA-dependent ROS production
Authors: Gao Yuxuan , Yan Wen , Sun Liangye , Zhang XiaoLing 
Published: March 2023

Abstract
Objectives
As a new molecular marker and therapeutic target, Piwi-interacting RNA(piRNA) has been widely studied in the field of oncology, but few people have studied the role of piRNA in osteoarthritis. Here, we found that the expression of hsa_piR_019914 in human osteoarthritis cartilage decreased significantly. We studied the relationship between hsa_piR_019914 and gene expression in osteoarthritis cartilage and the role of hsa_piR_019914 in chondrocyte biology. It is suggested that hsa_piR_019914 may be a new target for the treatment of osteoarthritis.
Design
Using the GEO database to analyze the expression of piRNA in the cartilage of osteoarthritis, using qPCR to detect the effect of inflammatory factors on the expression of piRNA in chondrocytes, using CCK-8 and clone formation to detect the effect of hsa_piR_019914 on chondrocyte proliferation, and using flow cytometry to detect the effect of hsa_piR_019914 on chondrocyte apoptosis and ROS production. The target gene of hsa_piR_019914 regulation was detected by mRNA sequencing.
Results
GEO data analysis found that 11 piRNAs were downregulated in cartilage tissue of osteoarthritis, and inflammatory factors inhibited the expression of hsa_piR_019914 in chondrocytes. Hsa_piR_019914 promoted the proliferation of chondrocytes and the synthesis of extracellular matrix. Hsa_piR_019914 inhibited the apoptosis of chondrocytes and the expression of cell-matrix protease. Hsa_piR_019914 targets regulating the expression of LDHA and inhibiting the production of ROS.
Conclusions
This work highlights the role of hsa_piR_019914 in chondrocyte metabolism, the inhibition of LDHA, and the reduction of ROS production, which may be used as a potential treatment for osteoarthritis.
Electroacupuncture versus sham electroacupuncture in the treatment of female stress urinary incontinence: a randomized controlled trial protocol
Authors: Wang Xi , Hu Junwei , Song Xiuling , Ming Shuren , Li Juanjuan , Xu Ge , Zhang Xia , Chen Yuelai 
Published: March 2023

Abstract
Background: Stress urinary incontinence (SUI) is defined as involuntary leakage of urine due to increased abdominal pressure. Previous studies have confirmed that electroacupuncture is an effective alternative approach for treating SUI; yet, objective evidence of its mechanism of action is still lacking. Patients with SUI often harbor structural changes in the pelvic floor, which can be easily detected on transperineal ultrasound (TPUS). The aim of this study was to assess the effect of electroacupuncture involving the lower abdomen, compared with sham electroacupuncture in women with SUI, and observe changes in pelvic floor structure by TPUS.
Methods: Seventy-four female patients with SUI will be included in this study and randomly assigned to the test and control groups at a 1:1 ratio. Both groups will be treated with pelvic floor muscle training. In addition,patients in the test group will be subjected to electroacpuncture (EA) (at Zhongji (RN3), Guanyuan (RN4), and Dahe (KI12) acupoints), while the control group will be subjected to sham electroacupuncture (SEA) at same acupoints. Bothgroups will be treated three times a week for 6 weeks. The primary outcomes include change from baseline to week 6 in the bladder neck descent (BND) and the urine leakage measured by the 1-h pad test. Secondary outcomes include an objective degree of incontinence, subjective severity of incontinence, ICIQ-SF score, mean 24-h incontinence episodes, and subjective evaluation of incontinence leakage degree. TPUS will be used to observe the changes of parameters on the pelvic floor structure, such as urethral rotation angle (URA), retrovesical angle (RVA), the bladder neck position (BN-S), urethral funneling, and bladder bulging. The safety evaluation, and participant acceptance evaluation of the treatment will also be performed.
Discussion: The study may help objectively document the efficacy of electroacupuncture in SUI and clarify its mechanism of action.
Trial registration: Registration in the Chinese Clinical Trial Registry (ChiCTR2100050792)(http://www.chictr.org.cn/showproj.aspx?proj=132759),was effective September 4, 2021.
Just-in-time Adaptive Intervention for Stabilising Sleep Hours of Japanese Office Workers: A Micro-randomised Trial
Authors: Takeuchi Hiroki , Ishizawa Tetsuro , Kishi Akifumi , Nakamura Toru , Yoshiuchi Kazuhiro , Yamamoto Yoshiharu 
Published: March 2023

Abstract
Sleep disturbance is a major contributor to future health and occupational issues. Mobile health (mHealth) can provide interventions that address adverse health behaviours for individuals in vulnerable health state, in real-world settings (just-in-time adaptive intervention, JITAI). Here, Japanese office workers were instructed to collect data on their habitual sleep behaviours (including sleep hours, midpoint of sleep, and sleep efficiency) and momentary symptoms (including depressive mood, anxiety, and subjective sleep quality) using digital devices in a real-world setting. In study 1, we utilised these data to identify a subpopulation that had problems in habitual sleep behaviours and found that unstable sleep hours were associated with disturbed momentary symptoms. Based on these findings, in study 2, we designed and conducted a sleep JITAI, which delivered objective push-type sleep feedback for stabilising their sleep hours, for some participants in study 1. Moreover, we employed micro-randomisation to examine the within-individual proximal effects of the intervention. Providing sleep feedback prolonged subsequent sleep hours, but this effect was attenuated over time. Changing sleep hours improved depressive mood and subjective sleep quality. Participants in study 2 had more stabilised sleep hours than those in study 1. This is the first mHealth study to demonstrate that sleep feedback proximally and distally improves sleep behaviours in a micro-randomised trial. The findings of this study encourage the use of digitalised health intervention trials that employs real-time health monitoring and personalised feedback.
Human papillomavirus self-sampling and urine-sampling tests and the management and short-term outcomes of cervical intraepithelial neoplasia: A prospective observational study
Authors: Matsuura Motoki , Tamate Masato , Nagao Sachiko , Kurokawa Shoko , Nishimura Yoko , Isoyama Kyoko , Akimoto Taishi , Kasuga Fukiko , Shimada Hiroshi , Wada Nagisa , Shikanai Satoshi , Jin Miona , Tabuchi Yuta , Teramoto Mizue , Saito Tsuyoshi 
Published: March 2023

Abstract
Background: The importance of human papillomavirus (HPV) co-testing using physician-, self-, and urine-collected samples to predict cervical intraepithelial neoplasia (CIN) grade 1–2 prognoses has not been previously reported. Therefore, this study aimed to investigate the outcomes of patients with CIN 1–2 who simultaneously underwent physician-, self-, and urine-collection sampling tests.
Methods: This study was conducted in Japan between October 2019 and November 2022 and examined the proportion of cases with CIN 1–2 progressions, the percentage of cases with persistent CIN 1–2, and the outcome differences according to the results of physician-, self-, and urine sampling tests.
Results: There were 105 and 59 CIN 1 and 2 cases, with progression or persistence in 27 (29.3%) and 21 (50.0%) cases, respectively. The median follow-up was 20 and 12 months, respectively. Progression and persistence of CIN 1 were significantly associated with HPV-positive physician- and self-collection samples. No significant difference was observed between cases with CIN 2 who had HPV-positive and HPV-negative results using any sampling method.
Conclusions: Physician- and self-testing for HPV are crucial for predicting disease progression risk in CIN 1 cases. Future research with an extended observation period and consideration of the progression risks is warranted.
Annotation of Trauma-related Linguistic Features in Psychiatric Electronic Health Records for Machine Learning Applications
Authors: Holderness Eben , Atwood Bruce , Verhagen Marc , Shinn Ann , Cawkwell Philip , Pustejovsky James , Hall Mei-Hua 
Published: March 2023

Abstract
Psychiatric electronic health records (EHRs) present a distinctive challenge in the domain of ML owing to their unstructured nature, with a high degree of complexity and variability. This study aimed to identify a cohort of patients with diagnoses of a psychotic disorder and posttraumatic stress disorder (PTSD), develop clinically-informed guidelines for annotating these health records for instances of traumatic events to create a gold standard publicly available dataset, and demonstrate that the data gathered using this annotation scheme is suitable for training a machine learning (ML) model to identify these indicators of trauma in unseen health records. We created a representative corpus of 101 EHRs (222,033 tokens) from a centralized database and a detailed annotation scheme for annotating information relevant to traumatic events in the clinical narratives. A team of clinical experts annotated the dataset and updated the annotation guidelines in collaboration with computational linguistic specialists. Inter-annotator agreement was high (0.688 for span tags, 0.589 for relations, and 0.874 for tag attributes). We characterize the major points relating to the annotation process of psychiatric EHRs. Additionally, high-performing baseline span labeling and relation extraction ML models were developed to demonstrate practical viability of the gold standard corpus for ML applications.
Association of a decreased platelet count with poor survival in patients with adult secondary hemophagocytic lymphohistiocytosis
Authors: Cheng Wanying , Xu Ji , Shu Yongqian , Qiu Hongxia , Yin guangli 
Published: March 2023

Abstract
Purpose
We aimed to examine the association between baseline platelet count (PLT) and the prognosis of adult secondary hemophagocytic lymphohistiocytosis (sHLH).
Material and methods
Data from 292 patients with pretreatment platelet counts were retrospectively analysed from January 2016 to December 2020. We categorized platelet count into quartiles. Multivariable Cox proportional hazards models and restricted cubic splines (RCS) were used to evaluate the relationship between platelet count and mortality.
Results
During a median follow-up of 53 (interquartile ranges, 17–223) days, a total of 208 deaths occurred. After multivariable adjustment, a nonlinear and inverse relationship was observed for mortality (P for nonlinearity = 0.002). For non-LHLH, a similar curve was also observed (P for nonlinearity = 0.028). Decreased PLT ((PLT Q4)) was associated with an increased risk of mortality (adjusted hazard ratio: 1.97; 95% confidence interval: 1.28–3.04; Ptrend =0.005). Similar results were observed in the LHLH subgroup (adjusted hazard ratio: 1.84; 95% confidence interval: 1.05–3.24; Ptrend =0.024) but not in the non-LHLH subgroup (Ptrend =0.266).
Conclusions:
Baseline platelet count demonstrated a nonlinear and inverse association with an increased risk of mortality among adult sHLH patients. This method is used to identify sHLH patients with inferior overall survival due to its low cost and universal availability.