bluuebunny/crossref_metadata_2025_split huggingface dataset:
The complete mitochondrial genome of the freshwater crab Tzotzilthelphusa villarosalensis Villalobos & Álvarez, 2013 (Brachyura: Decapoda: Pseudothelphusidae: Raddausinae) from Mexico
Authors: Moreno-Juárez Eric G , Jiménez-Marin Andrea , Vera-Paz Sandra I , Álvarez Fernando 
Published: June 2023

Abstract
We present the complete mitochondrial genome of Tzotzilthelphusa villarosalensisVillalobos & Álvarez, 2013, a freshwater crab from Mexico. This is the first mitochondrial genome reported for the superfamily Pseudothelphusoidea. We performed a de novo assembly from genome skimming reads. The mitochondrial genome of T. villarosalensis has a length of 17,203 bp and its gene topology is similar to other freshwater and marine families, e.g., the position of the cytochrome oxidase complex subunits and ATA synthetase, even the order of nad3, nad5, nad4, nad4L, nad6, and cytb. By contrast, the order and organization of genes like the 16S, 12S, and nad1 are different depending on the phylogenetic group with which they are compared. The phylogenetic analyses placed T. villarosalensis as a sister group of all freshwater crabs reported until now and as part of the subsection Heterotremata. This complete mitochondrial genome could be a reference for further genomic studies of the family Pseudothelphusidae and other primary freshwater crabs.
Insecticide resistance and its potential mechanisms in field-collected German cockroaches (Blattodea: Ectobiidae) from Thailand
Authors: Tisgratog Rungarun , Panyafeang Chanikarn , Lee Shao-Hung , Rust Michael K , Lee Chow-Yang 
Published: June 2023

AbstractWe investigated insecticide resistance profiles of field populations of the German cockroach, Blattella germanica (L.), collected from central regions of Thailand. Seven strains (PW, RB, MTH, MTS, TL, AY, and SP) were evaluated with diagnostic doses (DD; 3 × LD95 generated from a susceptible strain) of deltamethrin, fipronil, and imidacloprid using topical assays and compared with a susceptible strain (DMSC). Results showed fipronil (2–27% mortality), deltamethrin (16–58% mortality), and imidacloprid (15–75% mortality) resistance in the field strains. Synergism studies with piperonyl butoxide (PBO) and S,S,S-tributyl phosphorotrithioate (DEF) in combination with the DD of insecticides significantly increased (P &lt; 0.05) mortality of the test insects of the field strains suggesting the involvement of P450 monooxygenase and esterase pathways of detoxification. Gel bait evaluations demonstrated that all field-collected strains were resistant to Maxforce Forte (0.05% fipronil), Maxforce Fusion (2.15% imidacloprid), and Advion Cockroach Gel Bait (0.6% indoxacarb) with mean survival times ranging from 1.87–8.27, 1.77–11.72, and 1.19–3.56 days, respectively. Molecular detection revealed that the Rdl mutation was completely homozygous in all field-collected strains except in the PW strain. Field-collected strains were screened for 3 voltage-gated sodium channel (VGSC) mutations associated with pyrethroid resistance. The L993F mutation was present in 5 strains, but no C764R and E434K mutations were detected.
Efficacy and nontarget effects of broadcast treatments to manage spotted lanternfly (Hemiptera: Fulgoridae) nymphs
Authors: Keller Joseph A , Walsh Brian , Johnson Anne , Jenkins Nina , Rost John , Treichler Brianna , Biddinger David , Calvin Dennis D , Hoover Kelli , Urban Julie , Roush Richard T 
Published: June 2023

Abstract
Management to control the spotted lanternfly, Lycorma delicatula (White), would ideally achieve managers’ goals while limiting impacts on nontarget organisms. In a large-scale field study with 45 plots at least 711 m2, we tested foliar applications of dinotefuran and 2 formulations of Beauveria bassiana (Balsamo) Vuillemin, each applied from the ground and separately by helicopter. Applications targeted early instar nymphs. For both application methods, a single treatment with dinotefuran significantly reduced L. delicatula numbers, as measured by catch on sticky bands (91% reduction by air and 84% reduction by ground 19 days after application) and by timed counts (89% reduction by air and 72% reduction by ground 17 days after application). None of the B. bassiana treatments significantly reduced L. delicatula numbers, even after 3 applications. Beauveria bassiana infection in field-collected nymphs ranged from 0.4% to 39.7%, with higher mortality and infection among nymphs collected from ground application plots. Beauveria bassiana conidia did not persist for long on foliage which probably contributed to low population reduction. Nontarget effects were not observed among arthropods captured in blue vane flight intercept traps, San Jose Scale pheromone sticky traps or pitfall traps, but power analysis revealed that small reductions of less than 40% may not be detected despite extensive sampling of 48,804 specimens. These results demonstrate that dinotefuran can markedly reduce local abundance of L. delicatula with little apparent effect on nontarget insects when applied shortly after hatch, and that aerial applications can match or exceed the effectiveness of applications from the ground.
Forest Disturbances and Nonindustrial Forest Landowners: Management of Invasive Plants, Fire Hazards and Wildlife Habitats After a Hurricane
Authors: Clarke Mysha , Sharma Ajay , Stein Taylor , Vogel Jason , Nowak Jarek 
Published: June 2023

AbstractThere is limited research about the interacting impacts of hurricane damage and forest disturbances like invasive species and wildfires on nonindustrial forest land management. We administered a mail survey to 1,000 randomly selected nonindustrial private forest landowners in the Panhandle of Florida who owned forestlands in the counties most severely affected by Hurricane Michael. Results show that most landowners did little to no forest management to prepare for the hurricane. Following Hurricane Michael, 34% of respondents reported increased invasive plants whereas 82% experienced timber loss, 62% had damaged wildlife habitats, and 53% had increased vulnerability to wildfire hazards. Landowners who experienced timber damage from Hurricane Michael were more likely to reduce fire hazard, eliminate or reduce invasive plants, perform road and trail maintenance, improve wildlife habitat, reforest both salvage harvested and areas that were not salvage harvested, search for information on the internet about forest management, and pass their forestland to family.Study Implications: Forest disturbances such as hurricanes, invasive species, and wildfires are predicted to increase in severity and frequency, consequently affecting forest productivity, sustainability, and management. Despite significant impacts on forest lands, there is limited understanding of how these disturbances may affect nonindustrial private forest landowner management behaviors. This article highlights the attitudes, experiences, and perceptions of nonindustrial private forest landowners, including their awareness and overall forest management plans regarding invasive species control, wildlife habitat improvement, and reducing wildfire hazards after a hurricane. We assessed the important factors that contribute to landowners’ willingness to take action to address these interacting forest disturbances.
Clinical utility of whole-genome DNA methylation profiling as a primary molecular diagnostic assay for central nervous system tumors—A prospective study and guidelines for clinical testing
Authors: Galbraith Kristyn , Vasudevaraja Varshini , Serrano Jonathan , Shen Guomiao , Tran Ivy , Abdallat Nancy , Wen Mandisa , Patel Seema , Movahed-Ezazi Misha , Faustin Arline , Spino-Keeton Marissa , Roberts Leah Geiser , Maloku Ekrem , Drexler Steven A , Liechty Benjamin L , Pisapia David , Krasnozhen-Ratush Olga , Rosenblum Marc , Shroff Seema , Boué Daniel R , Davidson Christian , Mao Qinwen , Suchi Mariko , North Paula , Hopp Amanda , Segura Annette , Jarzembowski Jason A , Parsons Lauren , Johnson Mahlon D , Mobley Bret , Samore Wesley , McGuone Declan , Gopal Pallavi P , Canoll Peter D , Horbinski Craig , Fullmer Joseph M , Farooqi Midhat S , Gokden Murat , Wadhwani Nitin R , Richardson Timothy E , Umphlett Melissa , Tsankova Nadejda M , DeWitt John C , Sen Chandra , Placantonakis Dimitris G , Pacione Donato , Wisoff Jeffrey H , Teresa Hidalgo Eveline , Harter David , William Christopher M , Cordova Christine , Kurz Sylvia C , Barbaro Marissa , Orringer Daniel A , Karajannis Matthias A , Sulman Erik P , Gardner Sharon L , Zagzag David , Tsirigos Aristotelis , Allen Jeffrey C , Golfinos John G , Snuderl Matija 
Published: June 2023

Abstract

Background
Central nervous system (CNS) cancer is the 10th leading cause of cancer-associated deaths for adults, but the leading cause in pediatric patients and young adults. The variety and complexity of histologic subtypes can lead to diagnostic errors. DNA methylation is an epigenetic modification that provides a tumor type-specific signature that can be used for diagnosis.

Methods
We performed a prospective study using DNA methylation analysis as a primary diagnostic method for 1921 brain tumors. All tumors received a pathology diagnosis and profiling by whole genome DNA methylation, followed by next-generation DNA and RNA sequencing. Results were stratified by concordance between DNA methylation and histopathology, establishing diagnostic utility.

Results
Of the 1602 cases with a World Health Organization histologic diagnosis, DNA methylation identified a diagnostic mismatch in 225 cases (14%), 78 cases (5%) did not classify with any class, and in an additional 110 (7%) cases DNA methylation confirmed the diagnosis and provided prognostic information. Of 319 cases carrying 195 different descriptive histologic diagnoses, DNA methylation provided a definitive diagnosis in 273 (86%) cases, separated them into 55 methylation classes, and changed the grading in 58 (18%) cases.

Conclusions
DNA methylation analysis is a robust method to diagnose primary CNS tumors, improving diagnostic accuracy, decreasing diagnostic errors and inconclusive diagnoses, and providing prognostic subclassification. This study provides a framework for inclusion of DNA methylation profiling as a primary molecular diagnostic test into professional guidelines for CNS tumors. The benefits include increased diagnostic accuracy, improved patient management, and refinements in clinical trial design.
Differentiating between severe hypertension and thrombocytopenic purpura as the cause of thrombotic microangiopathy in a patient with Castleman disease: a case report and literature review
Authors: Feldman Itamar , Shapiro Dvorah S , Ellis Raphael , Friedman Reuven 
Published: June 2023

Abstract
Thrombotic microangiopathy (TMA) is a syndrome characterized by thrombosis in capillaries and arterioles, resulting in microangiopathic hemolytic anemia, thrombocytopenia and target organ injury. In TMA presenting with severe hypertension, it is difficult to determine whether the TMA is primary and due to thrombotic thrombocytopenic purpura (TTP) or secondary to severe hypertension. The response to antihypertensive medication favors the diagnosis of severe hypertension as the cause of TMA. Comorbid inflammatory disease supports the diagnosis of TTP-induced TMA. This case describes a 75-year-old woman with Castleman disease who presented with severe hypertension and TMA. She improved with hypertension therapy. However, ADAMST13 showed zero activity, and the diagnosis was TTP. In cases of TMA accompanied by severe hypertension, it is challenging to diagnose the cause of TMA. Even when there is a pronounced clinical response to lowering blood pressure, the diagnosis of TTP should be considered, particularly when an inflammatory disease is present.
Clinicogenomic factors and treatment patterns among patients with advanced non–small cell lung cancer with or without brain metastases in the United States
Authors: Smyth Emily Nash , John Jincy , Tiu Ramon V , Willard Melinda Dale , Beyrer Julie Kay , Bowman Lee , Sheffield Kristin M , Han Yimei , Brastianos Priscilla K 
Published: June 2023

Abstract

Background
This retrospective, real-world study evaluated the prevalence of brain metastases, clinicodemographic characteristics, systemic treatments, and factors associated with overall survival among patients with advanced non–small cell lung cancer (aNSCLC) in the US. We also described the genomic characterization of 180 brain metastatic specimens and frequency of clinically actionable genes.

Materials and Methods
De-identified electronic health records-derived data of adult patients diagnosed with aNSCLC between 2011 and 2017 were analyzed from a US-nationwide clinicogenomic database.

Results
Of 3257 adult patients with aNSCLC included in the study, approximately 31% (n = 1018) had brain metastases. Of these 1018 patients, 71% (n = 726) were diagnosed with brain metastases at initial NSCLC diagnosis; 57% (n = 583) of patients with brain metastases received systemic treatment. Platinum-based chemotherapy combinations were the most common first-line therapy; single-agent chemotherapies, epidermal growth factor receptor tyrosine kinase inhibitors, and platinum-based chemotherapy combinations were used in second line. Patients with brain metastases had a 1.56 times greater risk of death versus those with no brain metastases. In the brain metastatic specimens (n = 180), a high frequency of genomic alterations in the p53, MAPK, PI3K, mTOR, and cell-cycle associated pathways was observed.

Conclusion
The frequency of brain metastases at initial clinical presentation and associated poor prognosis for patients in this cohort underscores the importance of early screening for brain metastasis in NSCLC. Genomic alterations frequently identified in this study emphasize the continued need for genomic research and investigation of targeted therapies in patients with brain metastases.
Aspirin Use and Survival Among Patients With Breast Cancer: A Systematic Review and Meta-Analysis
Authors: Baker Adam , Kartsonaki Christiana 
Published: June 2023

Abstract

Background
Previous meta-analyses have indicated that aspirin could affect breast cancer outcomes, particularly when taken post-diagnostically. However, several recent studies appear to show little to no association between aspirin use and breast cancer mortality, all-cause mortality, or recurrence.

Aims
This study aims to conduct an updated systematic review and meta-analysis on the associations of pre-diagnostic and post-diagnostic aspirin use with the aforementioned breast cancer outcomes. It also looks, through subgroup analyses and meta-regressions, at a range of variables that could explain the associations between aspirin use and breast cancer outcomes.

Results
In total, 24 papers and 149 860 patients with breast cancer were included. Pre-diagnostic aspirin use was not associated with breast-cancer-specific mortality (HR 0.98, 95% CI, 0.80-1.20, P = .84) or recurrence (HR 0.94, 95% CI, 0.88-1.02, P = .13). Pre-diagnostic aspirin was associated with non-significantly higher all-cause mortality (HR 1.27, 95% CI, 0.95-1.72, P = .11). Post-diagnostic aspirin was not significantly associated with all-cause mortality (HR 0.87, 95% CI, 0.71-1.07, P = .18) or recurrence (HR 0.89, 95% CI, 0.67-1.16, P = .38). Post-diagnostic aspirin use was significantly associated with lower breast-cancer-specific mortality (HR 0.79, 95% CI, 0.64-0.98, P = .032).

Conclusions
The only significant association of aspirin with breast cancer outcomes is lower breast-cancer-specific mortality in patients who used aspirin post-diagnostically. However, factors such as selection bias and high inter-study heterogeneity mean that this result should not be treated as conclusive, and more substantial evidence such as that provided by RCTs is needed before any decisions on new clinical uses for aspirin should be made.
Protocol for a Parallel Group Randomized Clinical Trial Comparing a Culturally Adapted Cognitive Behavioral Telerehabilitation Intervention to Usual Physical Therapy for Latino Patients With Chronic Spine Pain
Authors: Gombatto Sara P , Archer Kristin R , Wegener Stephen T , Hernandez Yessenia , Lin Shih-Fan , Godino Job , Van Dyke Jason , Liu Jie , Monroe Katrina S 
Published: June 2023

AbstractObjectiveDisparities exist in health care access, diagnosis, and treatment of chronic pain in Latino populations and other minority populations. Cognitive behavioral–based physical therapy (CBPT) interventions have been shown to be effective in predominantly non-Hispanic white populations with chronic spine pain. However, there is a need for culturally adapted CBPT interventions that focus on the conservative management of chronic spine pain. The primary purpose of the study described in this protocol is to test the efficacy of an adapted cognitive behavioral–based hybrid telerehabilitation intervention for Latino patients with chronic spine pain.MethodsA single-blind, 2-arm parallel group, superiority randomized clinical trial is planned to compare an adapted CBPT intervention to Usual Care physical therapy. Goal Oriented Activity for Latinos with chronic Spine pain (GOALS/Metas) is an 8-week hybrid telerehabilitation intervention that integrates guideline-based physical therapy and pain management interventions using cognitive behavioral approaches and has been adapted for Latino patients with chronic spine pain. Usual Care physical therapy will be administered based on institutional standards at the referring health center. Outcome measures will be evaluated preintervention and at 1-week, 3-months, and 6-months postintervention. The primary outcome is pain-related disability 1-week postintervention using the Brief Pain Inventory Pain Interference subscale. Secondary outcome measures include behavioral measures of functional activity, social participation, physical activity, and sleep. Determinants of treatment effect, including pain-related psychological measures, posture and movement, self-efficacy, treatment expectancy, and therapeutic alliance, will be included in the secondary moderation and mediation analyses.ImpactThis clinical trial will provide information on the extent to which an adapted CBPT hybrid telerehabilitation intervention is effective in reducing pain-related disability for Latino patients with chronic spine pain. This information will be useful for clinicians to integrate in their practice, given the growing population of Latino patients who experience disparities in health care management of chronic pain.
The radioecological footprint of electricity production by wind turbines
Authors: Bittner Simon , Gellermann Rainer , Walther Clemens 
Published: June 2023

AbstractThe worldwide transformation of electricity production goes hand in hand with increasing use of wind energy. The German ‘Energiewende’ project is no exception and relies heavily on the construction and use of an ever-increasing number of wind turbines. While the operation of wind turbines does not lead to the emission of pollutants (in contrast to, e.g. coal, oil or gas), the production processes of the construction materials do. Since the raw materials’ production primarily takes place outside Germany, radioactivity and doses related to these processes occur at remote places in the world. This effect might be called an ‘export of doses’. In the present paper, we perform a life cycle analysis of wind turbines, investigating the mining and production of the construction materials. We focus on rare-earth elements needed for the generator magnets and assess the associated releases of radioactive materials during mining and processing, primarily in China. Estimates of dose to the public in selected Chinese cities are calculated. Different electricity generation techniques are compared by the use of the quantity (collective) dose per GW per year.
Social Psychopharmacology: Novel Approaches to Treat Deficits in Social Motivation in Schizophrenia
Authors: Bershad Anya K , de Wit Harriet 
Published: June 2023

Abstract

Background and Hypothesis
Diminished social motivation is a negative symptom of schizophrenia and leads to severe functional consequences for many patients suffering from the illness. However, there are no effective medications available to treat this symptom. Despite the lack of approved treatments for patients, there is a growing body of literature on the effects of several classes of drugs on social motivation in healthy volunteers that may be relevant to patients. The aim of this review is to synthesize these results in an effort to identify novel directions for the development of medications to treat reduced social motivation in schizophrenia.

Study Design
In this article, we review pharmacologic challenge studies addressing the acute effects of psychoactive drugs on social motivation in healthy volunteers and consider how these findings may be applied to deficits in social motivation in schizophrenia. We include studies testing amphetamines and 3,4-methylenedioxymethamphetamine (MDMA), opioids, cannabis, serotonergic psychedelics, antidepressants, benzodiazepines, and neuropeptides.

Study Results
We report that amphetamines, MDMA, and some opioid medications enhance social motivation in healthy adults and may represent promising avenues of investigation in schizophrenia.

Conclusions
Given the acute effects of these drugs on behavioral and performance-based measures of social motivation in healthy volunteers, they may be particularly beneficial as an adjunct to psychosocial training programs in patient populations. It remains to be determined how these medications affect patients with deficits in social motivation, and in which contexts they may be most effectively administered.
Feasibility study on epithelial cells regeneration after excising the demucosalized ileum for ureteral reconstructive surgery
Authors: Jian Yu , Gu Hao , Luo Ke‐Bing , Fu Rong‐bo , Zhao Ze‐Ju 
Published: June 2023

AbstractThis study aimed to investigate the regeneration of epithelial cells in the long‐term observation of ureter reconstruction by excising the demucosalized ileum. First, 8 Beagle dogs were anesthetized and the abdominal cavity was inspected for abnormalities via an abdominal incision. The right kidney and ureter were subsequently separated, and the ureter was severed from its connection to the renal pelvis and bladder and ligated distally. The 10–15 cm of ileum was used to reconstruct the ureter. The biopsies of the proximal, middle, and distal reconstructed ureter (neo‐ureter) were collected at the first, third, fifth, and sixth month postoperatively. The regeneration of ileal mucosa at the first, third, fifth, and sixth month was observed by hematoxylin–eosin (HE) staining and immunofluorescence staining cytokeratin 18 (CK18). HE staining results showed irregular cytoarchitecture, severe nuclear consolidation, and inflammatory infiltration in the proximal, middle, and distal neo‐ureters of dogs at the first month after ureteral reconstruction. With longer follow‐up, the injuries of the proximal, middle, and distal neo‐ureters were alleviated at the third, fifth, and sixth month after surgery. The expression of CK18 was higher in the middle neo‐ureters than that in the proximal and distal neo‐ureters at different time points after ureteral reconstruction and decreased with time. In summary, the present study demonstrated that demucosalized ileum was feasible for ureteral reconstructive surgery with satisfying prognostic effects.