bluuebunny/crossref_metadata_2025_split huggingface dataset:
Designed and validated novel allele-specific primer to differentiate Kernel Row Number (KRN) in tropical field corn
Authors: Mukri Ganapati , Shilpa Kumari , Gadag R. N. , Bhat Jayant S. , Singh Chandu , Gupta Navin C. , Prabha Chandra , Patil Sahana Police 
Published: April 2023

Background
Kernel row number (KRN) is an important yield component trait with a direct impact on the productivity of maize. The variability in KRN is influenced by the inflorescence meristem size, which is determined by the CLAVATA-WUSCHEL pathway. A CLAVATA receptor-like protein, encoded by the FASCIATED EAR2 (fea2gene), enhances the growth of inflorescence meristem and is thus involved in the determination of KRN. The amplicon sequencing-based method was employed to dissect the allelic variation of the fea2 gene in tropical field corn.

Methodology/Principal finding
Amplicon-based sequencing of AI 535 (Low KRN) and AI 536 (High KRN) was undertaken for the gene fea 2 gene that codes for KRN in maize. Upon multiple sequence alignment of both sequences, A to T transversion at the 1311 position was noticed between Low KRN and High KRN genotypes resulting in different allelic forms of a fea2 gene in tropical maize. An allele-specific primer 1311 fea2.1 was designed and validated that can differentiate High and Low KRN genotypes.

Conclusion/Significance
Maize has high variability for KRN and is exemplified by the wide values ranging from 8–26 KRN in the maize germpalsm. The sequence-based approach of SNP detection through the use of a specific primer facilitated the detection of variation present in the target trait. This makes it possible to capture these variations in the early generation. In the study, the PCR-based differentiation method described for the identification of desirable high KRN genotypes would augment the breeding programs for improving the productivity of field corn.
Desarrollo y caracterización de una milhojas de verdura lista para consumir enriquecida con polifenoles
Authors: Abalos Rosa Ana , Aviles M Victoria , Naef Elisa F , Gómez M. Beatriz 
Published: April 2023

Introduction: The development of high-quality plant-based ready-to-eat food options can respond to today's dietary demands. The trend of consumers opting for a plant-based diet is on the rise; however, they have few options for ready-to-eat products. The aim of this study was to develop a ready-to-eat plant product enriched with polyphenols using vacuum technologies.
Methodology: The physicochemical, sensory and microbiological characteristics of the product were evaluated. A lasagna was designed (layers of vegetables of regional origin -sweet potato (Ipomea batata) Gema variety, Creole zucchini (Curcubita maxima) and dried tomato- interspersed with a binder and cheese) ready to eat, which were vacuum impregnated with an extract of polyphenols. (95% oligomeric proanthocyanidins) from grape seeds. The concentration of polyphenols was measured by the Folin-Ciocalteu colorimetric method. The commercial life in refrigeration (0 - 3 °C) of the culinary preparation was determined, evaluating pH, color, texture and microbial count of aerobic and anaerobic psychrotrophic bacteria, lactic acid bacteria, molds and yeasts following techniques established in ISO standards. The sensory evaluation was carried out with regular consumers of ready-to-eat products. They evaluated the appreciation of sensory attributes and the general acceptability of the sample on a 9-point hedonic scale.
Results: This study demonstrated that the sous vide cooking method improved the refrigerated storage time of the preparation since the microbial load was maintained at safe levels. The sensory evaluation showed a positive acceptance by consumers for most of the sensory characteristics of the designed product.
Conclusions: the technologies used, sous vide and vacuum impregnation, could enhance the gastronomic quality of the product, preserve the organoleptic and nutritional properties and guarantee its safety. The designed product could be an option to incorporate ready-to-eat vegetable dish preparations into the market and thus contribute to increasing the consumption of vegetables.
Financiación: The work was carried out with the financial support of the University of Entre Ríos (UNER)
Application of Quality Assurance Management in Boarding Schools: Implications for Final School Examination Results
Authors: Ayuhan Ayuhan , Shofiyah Siti , Karimah Ummah 
Published: April 2023

Purpose–The objective of the present investigation was to scrutinize and elucidate the Quality of Boarding schools to foster the production of high-quality graduates. The study was conducted at Madrasah Aliyah Insan Cendekia Serpong and Dwiwarna Parung Senior High School.
Design/methods/approach–This study utilized qualitative methodology, which involved various data collection techniques such as field observations, interviews with key informants within the school setting, document analysis, library research, and examination of prior research findings. The collected data were analyzed using various techniques including data reduction, presentation, and verification.
Findings–The study findings indicate that Madrasa Aliyah Negeri Insan Cendekia Boarding School Serpong and Dwiwarna Senior High School Boarding School Parung surpassed national quality assurance benchmarks. Graduation chart data from 2012 to 2017 exhibited a positive trend in progress. Madrasa Aliyah Negeri Scholars had a 94% acceptance rate into state universities, 147 students accepted into prestigious public universities in Indonesia, four accepted into private colleges, and five being accepted into overseas institutions. Similarly, Dwiwarna high school had a 50% acceptance rate into public universities, with 39 students accepted into public universities, 42 accepted into private universities, and six accepted into foreign universities.
Research implications/limitations–The study highlights the importance of quality assurance management in improving the performance of boarding schools. Future research can investigate the impact of specific quality assurance management practices on the academic outcomes of boarding schools.
Originality/value–This Study provides unique insights into the impact of quality assurance management on a specific academic outcome, which can inform future research in the field of education.
Cervical Leiomyoma in Pre-Menopausal Woman: A Case Report
Authors: Reza Hanif , Andriani Nadila Arisa , Suwantari Saphira Ayu 
Published: April 2023

ABSTRACT Background: The most common pelvic tumors in women of reproductive age are uterine fibroids or known as leiomyomas, the frequency of cervical leiomyomas is about 1 to 2%. Leiomyoma which causes symptoms requires surgery, the most frequent surgical procedure is hysterectomy. Nevertheless, the management of cervical leiomyomas in premenopausal women remains a challenge in order to preserve the uterus whereas future pregnancy is still possible. Therefore, hysterectomy is not the preferred procedure. We present a case of a 47-year-old, multiparous woman with cervical leiomyoma that was treated with myomectomy and curettage to give an overview of how to treat cervical leiomyoma in patients who wanted to preserve their fertility.Case Presentation: A 47-year-old woman, P3A0, presented to the emergency department at Sultan Agung Islamic Hospital with a mass spontaneously protruding from the vagina a few hours before admission with a mass spontaneously protruding from the vagina, lower abdominal and heavy vaginal bleeding. The gynecological examination revealed a mass with a reddish surface and blood protruding out of the introitus. The dimensions of the mass were 20 cm x 8 cm x 12 cm. The mass is mobile, painful, tender on palpation, and seemed to be originating from the cervix. Myomectomy and curettage were performed. The histopathology result was suggestive of benign leiomyoma.Conclusion: In conclusion, this was a case of a patient with symptoms associated with cervical leiomyoma who was effectively treated with myomectomy and curettage. Myomectomy is considered the most frequent therapy for symptomatic myomas for women who desire to preserve their fertility.
Genetic Counseling in a Couple with Primary Infertility
Authors: Hadiprajitno Peter Ivan , Faradz Sultana 
Published: April 2023

Background: Couples unable to conceive and bear children could feel deep disappointment, often leading to depression. Infertility is one of the main reasons couples are not able to have children. Genetic counseling role in infertility ranges from explanation about possible genetic causes of infertility, pregnancy planning, and advice for treatment. Case Presentation: A couple with 16 years of infertility was referred to the genetic clinic at National Diponegoro Hospital. The 42 years old female had previous history of diabetes mellitus, obesity, and had treatment of epilepsy/seizure 15 years ago with routine carbamazepine therapy for 2 years, while her 42 years old husband had active hepatitis B infection for 15 years. This couple underwent insemination program twice and once completed In Vitro Fertilization (IVF), both management bearing no successful implantation or viable pregnancy. Recently, she underwent a laparoscopy procedure, which gave new diagnosis of endometriosis and adenomyosis. Chromosomal examination and Methylenetetrahydrofolate Reductase (MTHFR) C677T and A1298C alleles analysis were done in our laboratory. Both individuals carried normal karyotypes and MTHFR analysis was homozygote wild type allele. Currently, this couple has accepted their conditions. They still want to bear a child although she is at a crucial ageConclusion: Infertility is a challenging and comprehensive problem. As healthcare professionals, we encounter problems not only in diagnosis and management, but also psychological and emotional dilemma. Genetic counseling is needed to solve the problems and avoid patient’s psychological distress.
Late-life depression in primary care: Where do we go from here?
Authors: Banazak Deborah A. , Wills Celia , Collins Clare 
Published: April 2023

Abstract
One quarter of elderly patients in the primary care physician’s office experience serious depressive symptoms. Despite efforts over the past 20 years to increase detection of late-life depression in primary care settings, patient outcomes have not improved. Undertreatment remains seriously problematic. Current efforts to improve recognition have included the development of depression practice guidelines, Depression Awareness Recognition and Treatment (D/ART) program, educational programs, and rudimentary outcomes measures. Screening tools for depression, such as the Geriatric Depression Scale, the Center for Epidemiologic Studies—Depressed, and Cornell Scale for Depression in Dementia, have also been developed to help clinicians screen for depressive symptoms in both ambulatory and inpatient settings. However, to improve clinical outcomes, increased research efforts should focus upon physicians’ attitudes and practice patterns, effective treatments for minor depression, and effective ways to assess patients’ perceptions of depression, as well as ways to identify age-specific barriers to treatment adherence. In addition, incorporating valid outcome measures into the primary care clinical setting will be crucial to measure the impact of our treatments.
Itraconazole in the treatment of superficial cutaneous and mucosal Candida infections
Authors: Del Rosso James Q. , Zellis Sharon , Gupta Aditya K. 
Published: April 2023

Abstract
Superficial cutaneous and mucosal Candida infections are common and widely affect both the immunocompromised as well as the immunocompetent populations. Common infections may include Candida paronychia, cutaneous candidiasis, oral candidiasis, vulvovaginal candidiasis, and Candida onychomycosis. Although C albicans has been considered to be the most common pathogen, other Candida species have emerged as potential causes of certain infections. Currently, a variety of antifungal agents is available to treat these infections. They include fluconazole, ketoconazole, and itraconazole. These agents have been widely used to treat fungal infections, including superficial and systemic candidiasis. However, some concerns exist regarding safety associated with longterm use of ketoconazole, and emerging issues of Candida resistance to fluconazole in some patient subsets have been reported. Itraconazole has proven efficacy in treating cutaneous and mucosal Candida infections. Additionally, studies have demonstrated that itraconazole may have increased efficacy and an excellent safety profile when administered in a pulse-dose, or intermittent fashion, for superficial mycotic infections. Itraconazole is an effective agent that warrants consideration when selecting treatment for Candida infections.
Office-based preceptorship: A creative approach to a learning and growth experience
Authors: Silverberg Lawrence I. 
Published: April 2023

Abstract
Flexible 4-to-8-week medical student office rotations have both advantages and disadvantages, and they involve several different teaching issues. Among such issues are whether the medical student clerkship can be standardized, yet flexible; how students can gain a meaningful experience, and what this experience should include. Other considerations include the recruitment and retention of preceptors and the issues involved when a preceptor accepts a medical student. Benefits and disadvantages exist for the medical school, the preceptee, and the preceptor. The author outlines methods of evaluating the preceptorship and suggests appropriate recourse for situations in which the goals of preceptorship are not met. The questions raised warrant more research in this area.
Dual approval of a residency program: Ten years’ experience and implications for postdoctoral training
Authors: Hayes Oliver W. 
Published: April 2023

Abstract
In 1985, the American Osteopathic Association (AOA) changed its educational policies to permit osteopathic postdoctoral programs in settings that were accredited by the Accreditation Council for Graduate Medical Education (ACGME). In response to this policy change, an existing ACGME-accredited emergency medicine residency was modified to create a combined osteopathic/allopathic residency program that meets the educational requirements of both the AOA and the ACGME.
Our 10-year experience with this combined osteopathic/allopathic emergency medicine residency is described in terms of program development, governance structure involving key stakeholders, faculty distribution—equal numbers of allopathic and osteopathic faculty, and educational outcome of the total number of residents graduated (93 graduates, with 33 osteopathic physicians and 60 allopathic physicians). Lessons learned from the 10 years’ experience of this combined residency program, as well as current issues, are explained. The future potential of such dual-approved programs on the supply of residency positions is discussed from the viewpoint of osteopathic postdoctoral education.
Prosodically-driven morpheme non-realization in the Minorcan Catalan DP
Authors: Pons-Moll Clàudia , Torres-Tamarit Francesc , Mascaró Ignasi 
Published: April 2023

AbstractThis article focuses on a case of prosodically-driven morpheme non-realization found in the Minorcan Catalan DP that has not been documented or accounted for in the previous literature. In this variety of Catalan, kinship restrictive appositive phrases show the realization of the masculine personal article if the following personal name starts with a consonant (es conco en Toni, the-def.art.muncle the-pers.art.mToni ‘uncle Toni’), but not if it starts with a vowel (es conco Àngel, the-def.art.muncle Àngel ‘uncle Àngel’). This asymmetrical pattern is entirely unexpected since a preconsonantal coda is generated in the former cases (cf.es conco en Toni[əs.kòŋ.kun.tɔ́.ni]) and an onsetless syllable and a hiatus are generated in the latter (cf.es conco Àngel[əs.kòŋ.ku.án.ʒəl]). The structures obtained are indeed non-optimizing from a strictly syllabic point of view and stand as a clear case of antimarkedness. We argue that this asymmetric behavior is prosodic in nature, and is mainly driven by the need to keep the left edge of the prosodic word free of clitic material, without challenging basic syllabification constraints active in Catalan. We further justify how the cases under scrutiny shed new light on the typology of alignment constraints referred to the alignment between prosodic and lexical categories, on the nature of morpheme realization constraints, and on the morphosyntax-phonology interface.
Research progress of biopolymers combined with stem cells in the repair of intrauterine adhesions
Authors: Wang Zhujie , Jiang Juanjuan , Li Xingmiao , Chen Mo , Yu Mengjia , Guo Meijun , Wang Ning , Li Yangyang , Jiang Xiuxiu 
Published: April 2023

Abstract
Asherman syndrome (AS) refers to the loss of endometrial stem cells and matrix hyperplasia caused by endometrial basal layer injury. Its common clinical manifestations include narrowing of the uterine cavity, reduction in menstrual volume, abdominal pain, amenorrhea, and infertility. According to the cause of AS, treatment includes a mechanical barrier and functional recovery. Because the functional recovery of the endometrium depends on the regeneration of endometrial epithelial cells, in the absence of cell involvement, the effect of repair by mechanical removal of adhesions is limited. Recently, tissue engineering and stem cell therapy have achieved promising progress in the recovery of organ function. Biological scaffolds have been applied to encapsulated stem cells as a therapeutic strategy for repairing endometrial damage. This review explains the progress of intrauterine materials and stem cell combined biopolymers in the study of AS and emphasizes the evidence from animal and clinical trials.
A Transcription Factor Etv1/Er81 Is Involved in the Differentiation of Sweet, Umami, and Sodium Taste Cells
Authors: Ohmoto Makoto , Jyotaki Masafumi , Yee Karen K. , Matsumoto Ichiro 
Published: April 2023

AbstractTaste cells are maintained by continuous turnover throughout a lifetime, yet the mechanisms of taste cell differentiation, and how taste sensations remain constant despite this continuous turnover, remain poorly understood. Here, we report that a transcription factor Etv1 (also known as Er81) is involved in the differentiation of taste cells responsible for the preference for sweet, umami, and salty tastes. Molecular analyses revealed thatEtv1is expressed by a subset of taste cells that depend on Skn-1a (also known as Pou2f3) for their generation and express T1R genes (responsible for sweet and umami tastes) orScnn1a(responsible for amiloride-sensitive salty taste).Etv1CreERT2/CreERT2mice express Etv1 isoform(s) but not Etv1 in putative proprioceptive neurons as comparable to wild-type mice, yet lack expression of Etv1 or an isoform in taste cells. TheseEtv1CreERT2/CreERT2mice have the same population of Skn-1a-dependent cells in taste buds as wild-type mice but have altered gene expression in taste cells, with regional differences. They have markedly decreased electrophysiological responses of chorda tympani nerves to sweet and umami tastes and to amiloride-sensitive salty taste evoked by sodium cation, but they have unchanged responses to bitter or sour tastes. Our data thus show that Etv1 is involved in the differentiation of the taste cells responsible for sweet, umami, and salty taste preferences.
A family with type A insulin resistance syndrome caused by a novel insulin receptor mutation
Authors: Horikawa Osamu , Ugi Satoshi , Takayoshi Tomofumi , Omura Yasushi , Yonishi Maya , Sato Daisuke , Fujita Yukihiro , Fuke Tomoya , Hirota Yushi , Ogawa Wataru , Maegawa Hiroshi 
Published: April 2023

Summary
A 17-year-old boy was referred to our endocrinology clinic for a clinical investigation of hyperinsulinemia. An oral glucose tolerance test showed plasma glucose concentrations in the normal range. However, insulin concentrations were considerably elevated (0 min: 71 μU/mL; 60 min: 953 μU/mL), suggesting severe insulin resistance. An insulin tolerance test confirmed that he had insulin resistance. There was no apparent hormonal or metabolic cause, including obesity. The patient had no outward features of hyperinsulinemia, including acanthosis nigricans or hirsutism. However, his mother and grandfather also had hyperinsulinemia. Genetic testing showed that the patient (proband), his mother, and his grandfather had a novel p.Val1086del heterozygous mutation in exon 17 of the insulin receptor gene (INSR). Although all three family members have the same mutation, their clinical courses have been different. The onset of the mother’s diabetes was estimated at 50 years, whereas the grandfather developed diabetes at 77 years.

Learning points

Type A insulin resistance syndrome is caused by mutations in the insulin receptor (INSR) gene and results in severe insulin resistance.

Genetic evaluation should be considered in adolescents or young adults with dysglycemia when an atypical phenotype, such as severe insulin resistance, or a relevant family history is observed.

Clinical courses may differ even if the same genetic mutation is found in a family.
Age, Gender, and Preoperative LVEF Influence on ICU Length of Stay After CABG
Authors: Arviolla Rara Amourra , Rismawan Budiana , Erias Erlangga Muchammad 
Published: April 2023

Coronary artery bypass graft (CABG) surgery is a surgical therapy for coronary artery disease (CAD) patients who cannot be solely treated using pharmacological therapy. Patients undergoing CABG surgery require careful postoperative monitoring in the intensive care unit (ICU). This leads to the need for careful selection of patients due to the limited number of ICU beds available. A prolonged stay in ICU could delay surgery for other patients. This retrospective study analyzed how preoperative factors such as age, gender, and preoperative left ventricular ejection fraction (LVEF) may influence patient's length of stay (LOS) in the ICU. For this study, subjects were patients undergoing isolated CABG in Dr. Hasan Sadikin General Hospital Bandung, Indonesia, during the period of January 2019- December 2020 who were selected using the simple random sampling method. The subjects were categorized into <65 years old and ≥65 years old age groups; man and woman gender; preoperative LVEF of <40% and ≥40%; and prolonged ICU LOS (>96 hours) and non-prolonged ICU LOS (<96 hours). Deceased patients in the ICU were excluded. Results of the bivariate and multivariate analyses showed that age was the only factor (p-value of 0.017) that increased the risk of prolonged ICU LOS (OR of 3.34) after CABG surgery that was statistically significant. This study concluded that patient of old age (>65 years old) is at a higher risk of having prolonged ICU LOS after CABG; thus, a careful scheduling of patients for CABG surgery by age is important to prevent prolonged ICU LOS after CABG.
Ein Reinforcement-Learning-Ansatz für die Optimierung von Heizkurven
Authors: Huang Chenzi , Seidel Stephan , Pruvost Hervé , Bräunig Jan 
Published: April 2023

In diesem Beitrag wird das Potenzial einer intelligenten Vorlauftemperaturregelung für das Heizungsnetz eines modernen Bürogebäudes analysiert. Da das Gebäude mit einem Niedertemperatur-Fußbodenheizsystem und großen Fensterflächen ausgestattet ist, besteht die Gefahr einer Überhitzung der Räume an Tagen mit viel Sonnenenergie, jedoch kühlen Nächten. Durch eine Optimierung und Anpassung der Vorlauftemperatur mittels eines Reinforcement Learning Ansatzes – Q-Learning – kann die Überhitzung der Räume durch Sonneneinstrahlung im Vergleich zu der Standardheizkurve effektiv reduziert und damit der Gesamtkomfort verbessert werden. Die Integration der neuen Reglung am realen Gebäude wird mittels eines Handlungsempfehlungssystems realisiert.