bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of oxygen and oxidative stress on de novo acquisition of antibiotic resistance in E. coli and Lactobacillus lactis
Authors: Qi Wenxi , Jonker Martijs J. , Teichmann Lisa , Wortel Meike , Kuile Benno H. ter 
Published: July 2023

Abstract
Background: Bacteria can acquire resistance through DNA mutations in response to exposure to sub-lethal concentrations of antibiotics. According to the radical-based theory, reactive oxygen species (ROS), a byproduct of the respiratory pathway, and oxidative stress caused by reactive metabolic byproducts, play a role in cell death as secondary killing mechanism.
Results: To investigate whether oxygen and ROS affect de novoacquisition of antibiotic resistance, evolution of resistance was compared in E. coli wildtype and ΔoxyR strains under aerobic and anaerobic conditions. Since Lactococcus lactis (L. lactis) does not have an active electron transport chain (ETC) even in the presence of oxygen, and thus forms much less ROS, resistance development in L. lactis was to distinguish between oxygen and ROS. The resistance acquisition in E. coli wildtype under aerobic and anaerobic conditions did not differ much. However, the aerobically grown ΔoxyR strain gained resistance faster than the wildtype or anaerobic ΔoxyR. Inducing an ETC by adding heme increased the rate at which L. lactis acquired resistance. Whole genome sequencing identified crucial mutations involved in the acquisition of resistance. These mutations were specific for each antibiotic. The lexA mutation in ΔoxyR strain under aerobic conditions indicated that the SOS response was involved in resistance acquisition.
Conclusions: The concept of hormesis can explain the beneficial effects of low levels of ROS and reactive metabolic byproducts, while high levels are lethal. DNA repair and mutagenesis may therefore expedite development of resistance. Taken together, the results suggest that oxygen as such barely affects resistance development. Nevertheless, non-lethal levels of ROS stimulate de novo acquisition of antibiotic resistance.
A mHealth Intervention to Reduce Perceived Stress in Patients with Ischemic Heart Disease: Study Protocol for the Randomized, Controlled Confirmatory Intervention ‘mStress-IHD’ Trial
Authors: Lortz Julia , Rassaf Tienush , Jansen Christoph , Knuschke Ramtin , Schweda Adam , Schnaubert Lenka , Rammos Christos , Köberlein-Neu Juliane , Skoda Eva-Maria , Teufel Martin , Bäuerle Alexander 
Published: July 2023

Abstract
Background
Stress is highly prevalent in patients with ischemic heart disease (IHD) and is associated with lower health-related quality of life and impaired cardiovascular outcome. The importance of stress management is now recognized in recent guidelines for the management of cardiovascular disease. However, effective stress management interventions are not implemented in clinical routine yet. The development of easily disseminated eHealth interventions, particularly mHealth, may offer a cost-effective and scalable solution to this problem. The aim of the proposed trial is to assess the efficiency and cost-effectiveness of the mHealth intervention ‘mindfulHeart’ in terms of reducing stress in patients with IHD.
Methods and analysis:
This randomized controlled confirmatory interventional trial with two parallel arms has assessments at six measurement time points: baseline (T0, prior randomization), post treatment (T1) and four follow-ups at month 1, 3, 6, and 12 after intervention (T2, T3, T4, and T5). We will include patients with confirmed diagnosis of IHD, high-perceived stress, and use an internet-enabled smartphone. Patients will be randomized into two groups (intervention vs. control). The proposed sample size calculation allocates 128 participants in total. The primary analysis will be performed in the intention-to-treat population, with missing data imputed. An ANCOVA with the outcome at T1, a between-subject factor (intervention vs. control), as well as the participants’ pre-intervention baseline values as a covariate will be used. Different ANOVAs, regression and descriptive approaches will be performed for secondary analyses.
Discussion
The randomized controlled ‘mStress-IHD’ trial aims to assess the efficacy and cost-effectiveness of the mHealth intervention ‘mindfulHeart’ for reducing stress in patients with IHD. A successful stress reduction in IHD patients with an increase of quality of life could be an important component of secondary prevention. Thus, the investigation related to the impact of the mHealth solution ‘mindfulHeart’ can lead to beneficial findings.
Ethics:
The Ethics Committee of the Medical Faculty of the University of Duisburg-Essen approved the study (22-11015-BO).
Trial registration number:
ClinicalTrials identifier NCT05846334
Tango-therapy vs physical activity in older people with dementia. A randomized controlled trial
Authors: Bracco Lucia , Pinto-Carral Arrate , Hillaert Linda , Mourey France 
Published: July 2023

Abstract
Background: dementia is a growing health concern that affects millions of people worldwide. Balance and gait disorders are often present and represent a major risk factor for falls. We investigated the effectiveness of tango-therapy in older people with dementia.
Methods: A randomised controlled trial with 31 participants living in a specialised Alzheimer disease unit, aged 65 to 93 years old, who were randomly assigned to tango group (IG) or adapted physical activity group (CG). Physical abilities (gait speed, Timed Up and Go test, Short Physical Performance Battery), ability to perform activities of daily living (Katz Index) and quality of life (Quality of life in Alzheimer Disease) were assessed. Measurements were performed at baseline, and after one and three months of training.
Results: after 3 months IG improved gait speed (p=0.035) while CG worsened (p=0.273), implying a statistically significant difference between groups (p=0.049). Both groups declined in their ability to perform activities of daily living, being statistically significant only in the CC (p=0.004). Both groups improved their quality of life, although this result was not constant during the 3-month intervention.
Conclusion: Tango interventions showed efficacy in improving gait and functional mobility, as well as in attenuating the deterioration in capacities of ADL skills. Allowing older people with dementia access to non-pharmacological interventions may be a successful strategy to prevent functional decline.
Trial registration: Tango for Older People with Dementia. Registered at ClinicalTrials.gov (ID: NCT05744011). First trial registration: 24/02/2023
Longitudinal propagation of aquatic disturbances from the largest wildfire recorded in New Mexico, USA
Authors: Nichols Justin , Kaphle Asmita , Tunby Paige , Khandelwal Aashish , Reale Justin , Horn David Van , González-Pinzón Ricardo 
Published: July 2023

Abstract

Wildfires generate hydro-geomorphological disturbances initiated by post-fire precipitation-runoff events. These disturbances are drivers of aquatic impairment over multiple months and years. While the impacts of wildfires on streams and rivers near burned areas have received increased attention in the last decades, it is still unclear how wildfire disturbances propagate longitudinally through fluvial networks. We deployed a rapid response team to install and maintain five high-frequency in-situ sondes to monitor water quality and stream metabolism changes over 190 km along the Gallinas Creek-Pecos River-Santa Rosa Lake fluvial network in response to the Hermit’s Peak - Calf Canyon (HPCC) wildfire, the largest in New Mexico’s recorded history. We assessed how far downstream water quality disturbances propagated along the fluvial network, the role of seasonality in that propagation, and the impact of lakes in mitigating further longitudinal propagation. Monitoring began a few days after the fire started in April 2022 and before any precipitation events had occurred. In the ten months post-fire, there were significant increases in turbidity and fDOM and reductions in gross primary production and ecosystem respiration at all monitoring sites upstream of Santa Rosa Lake. Stream metabolic fingerprints suggest increased scouring, DOC, nutrients, and suspended sediments at these sites. In contrast, the site downstream of Santa Rosa Lake did not have altered turbidity, gross primary production, or ecosystem respiration, and the metabolic fingerprints remained unchanged. These results suggest that Santa Rosa Lake, and associated water operations, buffered the propagation of wildfire disturbances ~180km downstream from the burn scar, resetting water quality parameters and metabolic activity for over ten months post-fire.
Ofatumumab as Part of Reduced Intensity Conditioning in High Risk B-cell Lymphoma Patients: Final Long-term Analysis From a Prospective Multicenter Phase-ii Trial
Authors: Cabrero Mónica , Corral Lucía López , Jarque Isidro , Cruz Fátima de la , Valcárcel David , Pérez Estefanía , Orti Guillermo , Martín Carmen , Caballer Jaime Sanz , Serna Javier de la , Espigado-Tocino Ildefonso , Caballero Maria Dolores 
Published: July 2023

Abstract
Curative potential of allogeneic transplantation (AlloSCT) in high-risk non-Hodgkin lymphoma (NHL) could be enhanced by the integration of Ofatumumab (OFA), a 2nd generation anti-CD20 moAb, due to an antitumor effect and a role over graft-versus-host disease (GVHD). In this phase II trial (NCT01613300), we investigated safety and effectiveness of OFA-based reduced intensity conditioning (RIC). High-risk B-cell NHL patients with chemorrefractory disease or post-autologous SCT relapse were eligible. OFA was added to a standard RIC regimen. Primary endpoint was grade 3–4 aGVHD rate, while secondary endpoints included CR and survival rates. Thirty-three patients were included (median age 51; diffuse large B-cell:68%, HLA-identical donor: 74%). No grade > 2 OFA toxicity was observed. Acute GVHD affected 77% of patients (16% grade 3–4). Remarkably, 75% achieved CR after first-line treatment. Chronic GVHD, primarily mild or moderate, occurred in 54% of patients. The CR rate at day + 100 was 81%. Relapses occurred in 7 patients after a median of 3 months. Causes of death were lymphoma progression (5), infections (10), and GVHD (2). At 24 months, progression-free and overall survival rates were 50%. OFA-RIC regimen is safe and effective, though acute GVHD remains a significant complication. However, data suggest that OFA could mitigate its severity.
Prevalence of patient-reported perioperative anaesthesia-related anxiety, anxiety triggers and its association with patient satisfaction: a retrospective, observational study in Switzerland
Authors: Bello Corina , Nuebling Matthias , Koster Kira-Lee , Heidegger Thomas 
Published: July 2023

Abstract
Perioperative anxiety is a widespread complaint. The mutual relation between anxiety and patient satisfaction with anaesthesia is still under debate. We assessed the prevalence and different triggers of perioperative anxiety and the association with patient satisfaction. A psychometric questionnaire1 was sent to patients after discharge. Clinical Data was used from a previous study. Statistical analysis included bivariate and multivariate regression models. 141 patients (30%) reported anxiety regarding anaesthesia before admission to hospital. The prevalence of anxiety was significantly associated with patient age < 54 years (n = 196, prevalence = 37%, p = 0.002), female gender (n = 242, prevalence 39%, p < 0.001) and surgical specialty (gynaecology (n = 61, prevalence = 49%), otolaryngology (n = 56, prevalence = 46%) p < 0.001). The fear of not waking up from anaesthesia (n = 44, prevalence 32%, SD 45.8) and of developing postoperative nausea or vomiting (n = 42, prevalence 30%, SD 46.0) were the most reported triggers of anxiety. The presence of anxiety was associated with impaired overall patient satisfaction (mean dissatisfaction score 23%, SD 16.3, p < 0.001), especially regarding the dimensions “information and involvement in decision-making” (14% of deficits stated in non-anxious group compared to 23% in anxious group, p < 0.001), “respect and trust” (2% vs 6.26%, p < 0.001) and “continuity of care” (50% vs 57%, p < 0.015).
Sign Language Recognition and Training Module
Authors: V Anjana Devi , T Charulatha , P Dharishinie 
Published: July 2023

Abstract

Recognition of sign language has long been essential to communication between the deaf and non-verbal cultures. From early electric signal-based sign language identification to more recent recognition using machine/deep learning techniques, researchers from all over the world have made an effort to automate this process. Key point detection-based sign language recognition (SLR) is the major goal of this study. The focus of this work is American Sign Language (ASL), particularly ASL pickle data. Several machines learning algorithms, including random forest, support vector machine, and k nearest neighbour, were used to train the model. Lastly, the best model is selected from the model testing using assessment parameters including f1score, precision, and recall. To gather user input, a simple GUI is made, and the forecast is then made using the best machine learning model. Additionally, a training tool for learning American sign language has been developed, which will have a significant impact on non-verbal cultures.
Complex interventions and service innovations: development and application of the COMPLETE framework for patient-centered and justice-oriented design
Authors: May Carl R , Hillis Alyson , Gravenhorst Katja , Bradley Cory D , Geng Elvin , Gallacher Katie , Chew-Graham Carolyn A , Lippiett Kate , May Christine M , Smyth Rachel , Stevenson Fiona , Richardson Alison , Mair Frances , MacFarlane Anne , Montori Victor M 
Published: July 2023

Abstract

Background. The field of implementation research has recently seen considerable, and long overdue, interest in equity, with a strong emphasis on recognizing and responding to disparities in care. The COMPLETE (COMPLEx inTErventions and service innovation)framework is intended to support inclusive intervention and service design. The aim is the development of a patient-centered and justice-informed framework for the design of complex healthcare interventions and innovations in service design.Methods. We undertook three studies. (1) We operationalized constructs from sociological theories of inequality, implementation processes, social roles and expectations, to build a Matrix Model of mechanisms that drive the embedding of disparities in complex healthcare interventions and service innovations. (2) We developed patient-centered and justice-informed principles for design that form a Theory of Change that can be applied in intervention and service design. (3) We then linked the Matrix Model and the Theory of Change together with core elements of theories of prefigurative design and research justice, to build a Transformational Design Cycle for complex interventions and service innovations around a set of critical questions about the implications.Results. The COMPLETE framework focuses on the ways health inequalities and inequities are brought into being by systemic mechanisms within health services; are then propagated and normalized through implementation mechanisms; and are then realized and normalized in practice through institutionalized expectations of patients and caregivers. The framework provides tools to resist the naturalization of inequities and inequalities.Conclusion. We developed a framework for intervention and service design that consists of three components: a conceptual model of the translation of social determinants of health through organizational mechanisms that shape patient and caregivers’ lived experience of disparities in health and healthcare; a transformational theory of change; and a set of principles for design that seeks to place restitution for disparities at its center.
Fading SARS-CoV-2 humoral VOC cross-reactivity versus sustained cellular immunity in convalescent children and adolescents
Authors: Paniskaki Krystallenia , Goretzki Sarah , Anft Moritz , Konik Margarethe J. , Lechtenberg Klara , Vogl Melanie , Meister Toni L. , Pfaender Stephanie , Zettler Markus , Jäger Jasmin , Dolff Sebastian , Westhoff Timm H. , Rohn Hana , Felderhoff-Mueser Ursula , Stervbo Ulrik , Witzke Oliver , Dohna-schwake Christian , Babel Nina 
Published: July 2023

Abstract
Cross-reactive cellular and humoral immunity can substantially contribute to antiviral defense against SARS-CoV-2 variants of concern (VOC). While the adult SARS-CoV-2 cellular and humoral immunity and its cross-recognition potential against VOC is broadly analyzed, similar data regarding the pediatric population are missing. In this study, we perform an analysis of the humoral and cellular SARS-CoV-2 response immune of 32 convalescent COVID-19 children (children), 27 convalescent vaccinated adults(C+V+) and 7 unvaccinated convalescent adults (C+V-). SARS-CoV-2 reactive T cell response is analyzed via multiparametric flowcytometry and humoral immunity is addressed via pseudovirus neutralization assay. Similarly to adults, a significant reduction of cross-reactive neutralizing capacity against delta and omicron VOC was observed 6 months after SARS-CoV-2 infection. While SAR-CoV-2 neutralizing capacity was comparable among children and C+V- against all VOC, children demonstrated as expected an inferior humoral response when compared to C+V+. Nevertheless, children generated SARS-CoV-2 reactive T cells with broad cross-recognition potential. When compared to V+C+, children presented even comparable frequencies of WT-reactive CD4+ and CD8+ T cells with high avidity. Our results suggest that following SARS-CoV-2 infection children generate a humoral SARS-CoV-2 response with neutralizing potential comparable to unvaccinated COVID-19 convalescent adults as well a sustained SARS-CoV-2 cellular response cross-reactive to VOC.
Key statement
Following SARS-CoV-2 infection children generate a humoral SARS-CoV-2 response with neutralizing potential comparable to unvaccinated COVID-19 convalescent adults as well a sustained robust SARS-CoV-2 cellular response cross-reactive to VOC, comparable to vaccinated convalescent adults.
Gut Microbiota Changes in response to Medical Nutrition Therapy for Different Subtypes of Gestational Diabetes Mellitus
Authors: Han Mengyuan , Lin Xiang , Lin Lihua , Wu Zhihui , Lin Yingying , Dong Jiayi , Lin Jiaying , Xie Qianwen , Shen Qi , Lin Juan 
Published: July 2023

Abstract
Background
Our research aimed to analyze differences in gut microbiota composition among pregnant women with different GDM subtypes and evaluated the impact of medical nutrition therapy.
Methods
124 pregnant women, including 33 non-GDM and 91 GDM were enrolled. The GDM group was divided into 2 subgroups according to their 75 g oral glucose tolerance test (OGTT) results: abnormal glucose tolerance (GDM1, n=66) and elevated fasting blood glucose + abnormal glucose tolerance (GDM2, n=25). Fecal samples were collected from groups GDM1 and GDM2 after the confirmation of GDM diagnosis by OGTT at 24-28 weeks of pregnancy prior to medical nutrition therapy and again 8 weeks after commencement of medical nutrition therapy (32-36 weeks of pregnancy). Fecal samples were collected from non-GDM group at the corresponding gestational weeks. The compositions and changes of gut microbiota from different groups were analyzed and compared.
Results
Compared to GDM with fasting blood glucose combined with glucose tolerance abnormalities, the gut microbiota Alpha diversity of GDM with isolated glucose tolerance abnormalities is closer to the normal group. Beta diversity between three groups were significant different. The 8-week medical nutrition therapy did not cause significant changes in the gut microbiota composition of GDM patients, the Alpha diversity and Beta diversity had no statistical differences. LEfSe differential analysis showed that the abundance of Bifidobacterium, Anaerostipes, Streptococcus and Streptococcaceaesignificantly increased, while the abundance of Klebsiella, Enterobacteriaceae, Raoultella, and Acidaminococcaceae significantly decreased in group GDM1 after medical nutrition therapy. While the abundance of unclassified.Clostridiales, Turicibacter significantly increased, and the abundance of Phascolarctobacterium significantly decreased in group GDM2.
Conclusions
The gut microbiotacomposition of different subtypes of GDM had different characteristics. The medical nutrition therapy did not cause significant changes to gut microbiota composition of GDM patients but promoted increased abundance of beneficial Bifidobacterium.
Construction of ceRNA prognostic model based on the CCR7/CCL19 chemokine axis as a biomarker in breast cancer
Authors: Ma Rufei , Guan Xiuliang , Teng Nan , Du Yue , Ou Shu , Li Xiaofeng 
Published: July 2023

Abstract
Background
The study of CCR7/CCL19 chemokine axis and BC (BC) prognosis and metastasis is a current hot topic. We constructed a ceRNA network and risk-prognosis model based on CCR7/CCL19.
Methods
Based on the lncRNA, miRNA and mRNA expression data downloaded from the TCGA database, we used the starbase website to find the lncRNA and miRNA of CCR7/CCL19 and established the ceRNA network. The 1008 BC samples containing survival data were divided into Train group (504 cases) and Test group (504 cases) using R "caret" package. Then we constructed a prognostic risk model using RNA screened by univariate Cox analysis in the Train group and validated it in the Test and All groups. In addition, we explored the correlation between riskScores and clinical trials and immune-related factors (22 immune-infiltrating cells, tumor microenvironment, 13 immune-related pathways and 24 HLA genes). After transfection with knockdown CCR7, we observed the activity and migration ability of MDA-MB-231 and MCF-7 cells using CCK8, scratch assays and angiogenesis assays. Finally, qPCR was used to detect the expression levels of five RNAs in the prognostic risk model in MDA-MB-231 and MCF-7 cell.
Results
Patients with high expression of CCR7 and CCL19 had significantly higher overall survival times than those with low expression. The ceRNA network is constructed by 3 pairs of mRNA-miRNA pairs and 8 pairs of miRNA-lncRNA. After multivariate Cox analysis, we obtained a risk prognostic model: riskScore= -1.544 *`TRG-AS1`+ 0.936 * AC010327.5 + 0.553 *CCR7 -0.208 *CCL19 -0.315 *`hsa-let-7b-5p. Age, stage and riskScore can all be used as independent risk factors for BC prognosis. By drug sensitivity analysis, we found 5 drugs targeting CCR7 (convolamine, amikacin, AH-23848, ondansetron, flucloxacillin). After transfection with knockdown CCR7, we found a significant reduction in cell activity and migration capacity in MDA-MB-231 cells.
Conclusion
we constructed the first prognostic model based on the CCR7/CCL19 chemokine axis in BC and explored its role in immune infiltration, tumor microenvironment, and HLA genes.