bluuebunny/crossref_metadata_2025_split huggingface dataset:
Évaluation du burnout chez le personnel soignant de l'Institut de cancérologie d'Akanda (ICA)
Authors: Filankembo Kava A.C. , Ndjengue Bengono B.C. , Nzamba Bissielou P.L. , Nziengui C. , Kabena A. , Mpami T. , Belembaogo E. 
Published: June 2023

Objectif : Le syndrome d'épuisement professionnel est fréquent chez les travailleurs de la santé en oncologie. Non diagnostiqué et incorrectement pris en charge, le burnout peut avoir un impact négatif sur le rendement professionnel. L'Institut de cancérologie d'Akanda (ICA) est un centre hospitalier ultramoderne qui se veut une référence en matière de prise en charge du cancer en Afrique centrale. L'objectif de l'étude est de mesurer la fréquence du burnout au sein du personnel soignant de l'Institut de cancérologie d'Akanda et d'évaluer les principaux facteurs de risque.
Patients et méthodes : Nous avons mené une étude transversale à l'Institut de cancérologie d'Akanda durant le mois de janvier 2022. Le burnout a été évalué à l'aide du Maslach Burnout Inventory (MBI).
Résultats : Sur les 42 participants, on notait une prédominance féminine (57,1 %) avec un sex-ratio homme sur femme de 0,75. L'âge moyen était de 39,57 ± 8,13 ans. Les infirmiers représentaient plus de la moitié de l'échantillon (52,5 %). La moyenne d'années de service à l'ICA était de 6,30 ± 4,80 ans. Une proportion de 35,7 % des sujets présentaient un épuisement émotionnel. Quant à la dépersonnalisation sévère et la perte d'accomplissement sévère, elles étaient mesurées chez respectivement 14,3 et 31,0 % des participants. Le burnout sévère a été retrouvé chez 14,3 % des sujets. Il y avait une association significative entre l'épuisement émotionnel, les actes de violence et le soutien familial (p = 0,031 ; p = 0,042). Les sujets victimes d'actes de violence ont cinq fois plus de risque de développer un épuisement émotionnel sévère que les sujets non victimes. Les sujets bénéficiant d'un soutien familial ont une réduction du risque de 92,6 % de développer un épuisement émotionnel sévère. Il n'y avait pas d'association statistiquement significative entre les variables sociodémographiques ni professionnelles de l'échantillon et la dépersonnalisation ainsi qu'avec la perte d'accomplissement professionnel.
Conclusion : Le burnout est relativement fréquent à l'ICA. Il est important de pouvoir l'identifier afin de prévenir les effets néfastes qu'il peut avoir sur l'état de santé physique et mentale du personnel, pouvant être responsable d'un impact négatif sur le fonctionnement du service et la prise en charge des patients. Un plaidoyer doit être émis à l'encontre du directeur afin de mettre en place des campagnes de sensibilisation pour l'identification du trouble et d'offrir un suivi psychologique.
Metabolomic differentiation of tumor core versus edge in glioma
Authors: Baxter Mary E. , Miller Hunter A. , Chen Joseph , Williams Brian J. , Frieboes Hermann B. 
Published: June 2023

OBJECTIVE
Gliomas exhibit high intratumor and interpatient heterogeneity. Recently, it has been shown that the microenvironment and phenotype differ significantly between the glioma core (inner) and edge (infiltrating) regions. This proof-of-concept study differentiates metabolic signatures associated with these regions, with the potential for prognosis and targeted therapy that could improve surgical outcomes.

METHODS
Paired glioma core and infiltrating edge samples were obtained from 27 patients after craniotomy. Liquid-liquid metabolite extraction was performed on the samples and metabolomic data were obtained via 2D liquid chromatography–mass spectrometry/mass spectrometry. To gauge the potential of metabolomics to identify clinically relevant predictors of survival from tumor core versus edge tissues, a boosted generalized linear machine learning model was used to predict metabolomic profiles associated with O6-methylguanine DNA methyltransferase (MGMT) promoter methylation.

RESULTS
A panel of 66 (of 168) metabolites was found to significantly differ between glioma core and edge regions (p ≤ 0.05). Top metabolites with significantly different relative abundances included DL-alanine, creatine, cystathionine, nicotinamide, and D-pantothenic acid. Significant metabolic pathways identified by quantitative enrichment analysis included glycerophospholipid metabolism; butanoate metabolism; cysteine and methionine metabolism; glycine, serine, alanine, and threonine metabolism; purine metabolism; nicotinate and nicotinamide metabolism; and pantothenate and coenzyme A biosynthesis. The machine learning model using 4 key metabolites each within core and edge tissue specimens predicted MGMT promoter methylation status, with AUROCEdge = 0.960 and AUROCCore = 0.941. Top metabolites associated with MGMT status in the core samples included hydroxyhexanoycarnitine, spermine, succinic anhydride, and pantothenic acid, and in the edge samples metabolites included 5-cytidine monophosphate, pantothenic acid, itaconic acid, and uridine.

CONCLUSIONS
Key metabolic differences are identified between core and edge tissue in glioma and, furthermore, demonstrate the potential for machine learning to provide insight into potential prognostic and therapeutic targets.
Effectiveness of topical tacrolimus treatment in vitiligo patients
Authors: Basak Arpan Kumar , Debnath Joya , Das Anupam 
Published: June 2023

Background: Vitiligo is an acquired, pigmentary skin disorder that is disfiguring and difficult to treat. Phototherapy and the application of topical corticosteroids are most commonly prescribed. However, these therapies are often not effective and the use of corticosteroids on the face may lead to cutaneous atrophy, telangiectasia, and ocular complications.
Objective: To evaluate the effectiveness of topical tacrolimus treatment in vitiligo patients.
Materials and methods: This prospective, randomized control trial study was conducted among the patients who sought health care in the Dermatology and Venereology out patient department of KYAMC, Enayetpur, Sirajgonj from August 2021 to July 2022. The study was conducted with a view to evaluate the efficacy of topical tacrolimus in vitiligo and to see the adverse effects of topical tacrolimus in the treatment of vitiligo.
Results: This study shows 70 patients were enrolled them 32 were male and 38 were female. The mean age was 28.52±10.98 years. Monthly evaluations were performed. Maximum 61 patients (87.1%) achieved varying levels of repigmentation. 28 patients (40.0%) had reported up to 50% repigmentation. Sign and symptoms of erythema (8.6%) and burning (7.1%) were minimal.
Conclusion: This study revealed that tacrolimus ointment may be an effective and safe option for the treatment of vitiligo. The ease of topical self-administration with minimal side effects makes this novel immunomodulatory agent a promising addition to the therapeutic armamentarium for vitiligo.
Central Medical College Journal Vol 6 No 1 January 2022 Page: 44-48
A perfused multi-well bioreactor platform to assess tumor organoid response to a chemotherapeutic gradient
Authors: Wasson Elisa Marie , He Wei , Ahlquist Jesse , Hynes William Fredrick , Triplett Michael Gregory , Hinckley Aubree , Karelehto Eveliina , Gray-Sherr Delaney Ruth , Friedman Caleb Fisher , Robertson Claire , Shusteff Maxim , Warren Robert , Coleman Matthew A. , Moya Monica Lizet , Wheeler Elizabeth K. 
Published: May 2023

There is an urgent need to develop new therapies for colorectal cancer that has metastasized to the liver and, more fundamentally, to develop improved preclinical platforms of colorectal cancer liver metastases (CRCLM) to screen therapies for efficacy. To this end, we developed a multi-well perfusable bioreactor capable of monitoring CRCLM patient-derived organoid response to a chemotherapeutic gradient. CRCLM patient-derived organoids were cultured in the multi-well bioreactor for 7 days and the subsequently established gradient in 5-fluorouracil (5-FU) concentration resulted in a lower IC50 in the region near the perfusion channel versus the region far from the channel. We compared behaviour of organoids in this platform to two commonly used PDO culture models: organoids in media and organoids in a static (no perfusion) hydrogel. The bioreactor IC50 values were significantly higher than IC50 values for organoids cultured in media whereas only the IC50 for organoids far from the channel were significantly different than organoids cultured in the static hydrogel condition. Using finite element simulations, we showed that the total dose delivered, calculated using area under the curve (AUC) was similar between platforms, however normalized viability was lower for the organoid in media condition than in the static gel and bioreactor. Our results highlight the utility of our multi-well bioreactor for studying organoid response to chemical gradients and demonstrate that comparing drug response across these different platforms is nontrivial.
Novel homozygous variants in TTC12 cause male infertility with asthenoteratozoospermia owing to dynein arm complex and mitochondrial sheath defects in flagella
Authors: Meng Lanlan , Liu Qiang , Tan Chen , Xu Xilin , He Wenbin , Hu Tongyao , Tu Chaofeng , Li Yong , Du Juan , Zhang Qianjun , Lu Guangxiu , Fan Li-Qing , Lin Ge , Nie Hongchuan , Zhang Huan , Tan Yue-Qiu 
Published: June 2023

Introduction: Tracing the genetic causes for male infertility due to asthenoteratozoospermia has revealed at least 40 causative genes, which provides valuable reference for the genetic testing of asthenoteratozoospermia in clinical practice. To identify deleterious variants in the human tetratricopeptide repeat domain 12 (TTC12) gene in a large cohort of infertile Chinese males with asthenoteratozoospermia.Methods: A total of 314 unrelated asthenoteratozoospermia-affected men were recruited for whole exome sequencing. The effects of the identified variants were evaluated by in silico analysis, and confirmed by in vitro experiments. Intracytoplasmic sperm injection (ICSI) was used to evaluate the efficiency of assisted reproduction technique therapy.Results and Discussion: Novel homozygous TTC12 variants (c.1467_1467delG (p.Asp490Thrfs*14), c.1139_1139delA (p.His380Profs*4), and c.1117G&gt;A (p.Gly373Arg)) were identified in three (0.96%) of the 314 cases. Three mutants were indicated to be damaging using in silico prediction tools, and were further confirmed by in vitro functional analysis. Hematoxylin and eosin staining and ultrastructural observation of the spermatozoa revealed multiple morphological abnormalities of flagella, with the absence of outer and inner dynein arms. Notably, significant mitochondrial sheath malformations were also observed in the sperm flagella. Immunostaining assays indicated that TTC12 is present throughout the flagella, and was strongly concentrated in the mid-piece in control spermatozoa. However, spermatozoa from TTC12-mutated individuals exhibited almost no staining intensity of TTC12 and outer and inner dynein arms components. The three men accepted ICSI treatment using their ejaculated spermatozoa, and two female partners successfully delivered healthy babies. Our findings provide direct genetic evidence that homozygous variants in TTC12 cause male infertility with asthenoteratozoospermia by causing dynein arm complex defects and mitochondrial sheath malformations in the flagellar. We also demonstrated that TTC12 deficiency-mediated infertility could be overcome by ICSI technology.
Theoretical insights into the methane catalytic decomposition on graphene nanoribbons edges
Authors: Xavier Neubi F. , Payne Anthony J. R. , Bauerfeldt Glauco F. , Sacchi Marco 
Published: June 2023

Catalytic methane decomposition (CMD) is receiving much attention as a promising application for hydrogen production. Due to the high energy required for breaking the C-H bonds of methane, the choice of catalyst is crucial to the viability of this process. However, atomistic insights for the CMD mechanism on carbon-based materials are still limited. Here, we investigate the viability of CMD under reaction conditions on the zigzag (12-ZGNR) and armchair (AGRN) edges of graphene nanoribbons employing dispersion-corrected density functional theory (DFT). First, we investigated the desorption of H and H2 at 1200 K on the passivated 12-ZGNR and 12-AGNR edges. The diffusion of hydrogen atom on the passivated edges is the rate determinant step for the most favourable H2 desorption pathway, with a activation free energy of 4.17 eV and 3.45 eV on 12-ZGNR and 12-AGNR, respectively. The most favourable H2 desorption occurs on the 12-AGNR edges with a free energy barrier of 1.56 eV, reflecting the availability of bare carbon active sites on the catalytic application. The direct dissociative chemisorption of CH4 is the preferred pathway on the non-passivated 12-ZGNR edges, with an activation free energy of 0.56 eV. We also present the reaction steps for the complete catalytic dehydrogenation of methane on 12-ZGNR and 12-AGNR edges, proposing a mechanism in which the solid carbon formed on the edges act as new active sites. The active sites on the 12-AGNR edges show more propensity to be regenerated due lower free energy barrier of 2.71 eV for the H2 desorption from the newly grown active site. Comparison is made between the results obtained here and experimental and computational data available in the literature. We provide fundamental insights for the engineering of carbon-based catalysts for the CMD, showing that the bare carbon edges of graphene nanoribbons have performance comparable to commonly used metallic and bi-metallic catalysts for methane decomposition.
Advances in gut microbiome in metabonomics perspective: based on bibliometrics methods and visualization analysis
Authors: Wang Jieyan , Dong Peng , Zheng Shuqian , Mai Yiyin , Ding Jianan , Pan Pinfei , Tang Liugang , Wan Yantong , Liang Hui 
Published: May 2023

Background and aimsGastrointestinal microbial metabolomics is closely related to the state of the organism and has significant interaction with the pathogenesis of many diseases. Based on the publications in Web of Science Core Collection(WoSCC) from 2004 to 2022, this study conducted a bibliometric analysis of this field, aiming to understand its development trend and frontier, and provide basic information and potential points for in-depth exploration of this field.MethodsAll articles on gastrointestinal flora and metabolism published from 2004 to 2022 were collected and identified in WoCSS. CiteSpace v.6.1 and VOSviewer v.1.6.15.0 were used to calculate bibliometric indicators, including number of publications and citations, study categories, countries/institutions, authors/co-cited authors, journals/co-cited journals, co-cited references, and keywords. A map was drawn to visualize the data based on the analysis results for a more intuitive view.ResultsThere were 3811 articles in WoSCC that met our criteria. Analysis results show that the number of publications and citations in this field are increasing year by year. China is the country with the highest number of publications and USA owns the highest total link strength and citations. Chinese Acad Sci rank first for the number of institutional publications and total link strength. Journal of Proteome Research has the most publications. Nicholson, Jeremy K. is one of the most important scholars in this field. The most cited reference is “Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease”. Burst detection indicates that Urine, spectroscopy, metabonomic and gut microflora are long-standing hot topics in this field, while autism spectrum disorder and omics are likely to be at the forefront of research. The study of related metabolic small molecules and the application of gastrointestinal microbiome metabolomics in various diseases are currently emerging research directions and frontier in this field.ConclusionThis study is the first to make a bibliometric analysis of the studies related to gastrointestinal microbial metabolomics and reveal the development trends and current research hotspots in this field. This can contribute to the development of the field by providing relevant scholars with valuable and effective information about the current state of the field.
Oral Water Has Cardiovascular Effects Up to 60 min in Shock Patients
Authors: Guinot Pierre-Grégoire , Nguyen Maxime , Duclos Valerian , Berthoud Vivien , Bouhemad Belaid ,   The water study group
Published: May 2023

Aim: Little is known about the cardiovascular effects of oral water intake in shock patients. This study was designed to assess the effect of oral water on stroke volume and blood pressure during a 1-h time period.Method and Results: This open-label, randomized clinical trial included patients admitted to intensive care with acute circulatory failure. Three ICU units at the anesthesia and critical care department of the Dijon Bourgogne University Hospital. Patients were randomized 1:1 to an intervention or standard care group. The intervention group received 500 ml of oral water while the standard care group received intravenous administration of 500 ml of physiological saline solution. Baseline SV did not differ between the two groups (36 ml [28;51] vs. 38 ml [30;51], p = 0.952). The number of patients who were fluid responders did not differ between the two groups [n = 19 (76%) vs. n = 18 (72%), p = 1]. The median change in stroke volume during the three time points did not differ between the two groups (p &lt; 0.05). In the intervention group, blood pressure increased up to 60 min. In the control group, blood pressure quickly increased at the end of fluid expansion, then returned close to baseline value at 60 min.Conclusion: Shock patients who were administered oral water experienced improvements in blood pressure and blood flow up to 60 min when compared with patients who received intravenous saline solution. Further studies are warranted to confirm these effects.Clinical Trial Registration:www.clinicaltrials.gov, identifier: NCT03951519.
Crustal structure and its tectonic implications beneath the middle–lower Yangtze metallogenic belt in Anhui Province: 3D deep seismic sounding results from airgun source in inland waters
Authors: Guo Wenbin , Yang Zhuoxin , Tian Xiaofeng , Liu Baofeng , Zheng Chenglong , Song Xianghui , Gao Zhanyong , Qiu Yong 
Published: May 2023

To evaluate the effectiveness of using airguns as seismic sources in inland waters to detect the regional crustal structure, a mobile large-capacity airgun excitation experiment was conducted in October 2015 in the Anhui section of the middle–lower Yangtze metallogenic belt. In this study, we extracted 1,957 first-arrival phases (Pg) and 2,179 Moho reflection phases (PmP) from the airgun seismic signals, and performed joint inversion of the traveltimes. The inversion results reveal the P-wave high-velocity anomalies above 7 km depth in the upper crust beneath the ore clustering areas, suggesting the source of mineralized materials. The crustal velocity structure characteristics substantially differed above and below a depth of 7 km, indicating the existence of a regional basement detachment surface. The velocity structure in the middle–lower crust, especially in the lower crust show lateral uniformity characteristic, which could be related to that the middle–lower Yangtze metallogenic belt had undergone a MASH metallization process. The Moho is 30–36 km deep, and its uplift zone extends from southwest to northeast in a “V” shape, which is consistent with the planar spreading characteristics of the metallogenic belt, indicating that the asthenosphere uplift and crustal thinning have had a controlling effect on the formation of the metallogenic belt. This study suggests the present-day crust in the region along the Yangtze River in Anhui retains the traces of lithosphere delamination-thinning and basaltic magma underplating during the Yanshan period. Our results indicates that airgun source detection in inland waters can effectively determine the continental crustal structure.
Petrography, geochemistry and geochronology of igneous rocks from the Jiangnan Orogen, South China: constraints on the Early Paleozoic tectonic evolution of the South China Block
Authors: Tang Yuanyuan , Shi Yu , Weng Boyin , Zhou Yuxi , Lan Yuanchun 
Published: June 2023

The Early Paleozoic tectonic evolution of the South China Block (SCB) remains controversial related to intracontinental orogenic and oceanic subduction processes. We present whole-rock major and trace elemental data, LA-ICP-MS zircon U-Pb age and Lu-Hf isotopic data for the Early Paleozoic igneous rocks including granodiorites from the Yuechengling pluton and volcanic breccias from the Damingshan pluton from the western segment of the Jiangnan Orogen in the SCB. LA-ICP-MS zircon U-Pb dating yielded emplacement ages for the Yuechengling S-type granitoids of 438–436 Ma and the deposited age for the Damingshan volcaniclastic rocks to be later than 451 Ma. The Yuechengling granitoids have consistent SiO2, TiO2, Fe2O3T, MgO, and P2O5 contents, higher Al2O3 contents, and Na2O + K2O values, but lower Mg# values, compared with those of the Damingshan volcaniclastic rocks. All the studied samples exhibit enrichment in LREEs and moderate negative Eu anomalies (δEu = 0.63–0.75), with negative Ba, Sr, Nb, Ta, P, and Ti anomalies, and positive Rb, Th, U, Pb, and K anomalies. The granitoids have variable CaO/Na2O ratios of 0.22–1.11, negative εHf(t) values of −11.98 to −0.90, and corresponding TDM2 ages distributed from 2.37 to 1.55 Ga. The petrographic and geochemical characteristics of the Yuechengling granitoids indicate that their parental magma was derived from a crustal meta-greywacke and meta-pelite components in the Paleoproterozoic basement, and have undergone some degree of fractional crystallization. The volcaniclastic rocks have mostly negative εHf(t) values with partially positive (−27.54 to 8.73), and zircons with negative εHf(t) values (−27.54 to −0.14) show TDM2 ages of 3.79 to 1.63 Ga. Combined with petrographic and geochemical data, we suggest that the Damingshan volcaniclastic rocks were derived from Neoarchean-Neoproterozoic crustal materials and the felsic parental magma has undergone some degree of magma mixing with mantle material, and deposited soon after a Late Ordovician volcanic eruption (later than 451 Ma). Integrated with previous studies, our new data support the intracontinental orogenic model to account for the Early Paleozoic tectonic evolution. Thus, we suggest that the Early Paleozoic tectonic setting of the SCB was intracontinental orogeny rather than oceanic subduction-collision.