bluuebunny/crossref_metadata_2025_split huggingface dataset:
“There are still broken or fragmented systems”: Qualitative assessment of needs to strengthen adolescent mental health services in American Samoa
Authors: Mew Emma J. , Blas Vanessa , Winschel Julia , Hunt Leiema , Soliai‐Lemusu Si'itia , Johansson Astrid , Lowe Sarah R. , Faiai Mata'uitafa , Naseri Joshua , Toelupe Robert L. M. , Ipou Francine , Seui Moelili'a , Ledoux‐Sunia Trude , Sunia Fiafia , Galea’i Ariel , Poulin Rhayna , Hawley Nicola L. , McCutchan‐Tofaeono Jueta 
Published: September 2023

AbstractMental health challenges appear common among adolescents in American Samoa. There is a community‐identified need to better understand the health burden and identify opportunities to strengthen the mental health system to better meet adolescent mental health needs. The goal of this qualitative study was to gather community stakeholders' perceptions of common mental health problems among adolescents in American Samoa, identify existing services for adolescent mental health, and highlight strength‐based opportunities to enhance care. Using the Fa'afaletui research framework, semi‐structured in‐depth interviews (n = 28) were conducted between October 2020 and February 2021 using Zoom. Key informants were adults living in American Samoa, sampled for diversity in profession, age, gender, education, and region of residence. Transcripts were coded in duplicate and analysed using deductive thematic analysis. Participants reported that suicide, depression, anxiety, and post‐traumatic stress disorder are the conditions of highest burden. Participants identified an impressive amount of infrastructure and community mobilization, but also limitations in these services in addressing adolescents' health needs. Four themes arose related to the need to: (1) strengthen existing infrastructure; (2) reduce barriers to treatment access; (3) improve sustainability; and (4) encourage collaboration among leaders. Policymakers should prioritize the allocation of resources to strengthen infrastructure, public education, and stakeholder collaboration to improve adolescent mental health in American Samoa.
The influence of metal artifact reduction on the trueness of registration of a cone‐beam computed tomography scan with an intraoral scan in the presence of severe restoration artifact
Authors: Biun John , Dudhia Raahib , Arora Himanshu 
Published: September 2023

AbstractPurposeWhen planning guided implant surgery, highly radiopaque materials such as metals or zirconia produce streaking artifacts (‘metal artifact’) on cone‐beam computed tomography scans, which can impair registration of the intraoral scan. This study aimed to determine the effect of metal artifact reduction on the trueness of registration in the presence of multiple full‐coverage zirconia crowns.Materials and methodsA 3D‐printed maxillary study model was restored with 12 full‐coverage zirconia crowns and scanned with an intraoral scanner. Cone‐beam computed tomography scans of the study model were acquired, with and without activation of the metal artifact reduction algorithm. Registration of the optical scans was performed using initial point‐based registration with surface‐based refinement, and the deviation was measured at four pre‐defined dental landmarks. Welch's t‐test was used to compare the registration error for the metal artifact reduction group with the control group.ResultsThe average registration error was 0.519 mm (95% CI 0.507 to 0.531) with metal artifact reduction deactivated, compared to 0.478 mm (95% CI 0.460 to 0.496) without metal artifact reduction. Therefore, activation of the metal artifact reduction algorithm was associated with a 0.041 mm (95% CI 0.020 to 0.061, p < 0.001) increase in average registration error.ConclusionsThe use of the metal artifact reduction algorithm slightly reduced trueness in this in vitro study. Clinicians are advised not to rely on a metal artifact reduction (MAR) algorithm for registration of a cone‐beam computed tomography scan with an intraoral scan when planning guided implant surgery in the presence of restoration artifacts.
Changes in the skin microbiome during male maturation from 0 to 25 years of age
Authors: Hu Xinyue , Tang Meng , Dong Kun , Zhou Jin , Wang Dexian , Song Liya 
Published: September 2023

AbstractBackgroundSkin microorganisms co‐develop with the human body and age influences the skin microenvironment and thus the skin bacterial community.ObjectivesTo investigate the changes in the skin microbiota during male development.MethodsHigh‐throughput 16S ribosomal RNA pyrosequencing was utilized to analyze the differences in bacterial composition of the skin in healthy males aged 0–25 years.ResultsThere were significant differences in facial skin bacterial diversity (Shannon index) and richness (Chao index) among the 4 groups of subjects (p < 0.05). Streptococcus, Staphylococcus, Cutibacterium are dominant in males during growth, and regular changes occur with age after birth. Further analysis of skin bacteria between the 4 groups showed that the bacterial abundance of Cutibacterium acnes and Staphylococcus epidermidis tended to increase with age, and the bacterial abundance of Streptococcus, Rothia mucilaginosa, and Staphylococcus hominis tended to decrease with age.ConclusionsThere are some changes in cheek skin bacterial diversity during male development, and there is a relationship between skin bacterial changes and skin development processes.
Human immunodeficiency virus, hepatitis C virus and hepatitis B virus incidence in blood donors from 2000 to 2020 in France: Trends and lessons from haemovigilance surveillance
Authors: Laperche Syria , Sauvage Claire , Gallian Pierre , Jbilou Saadia , Pouchol Elodie , Py Jean Yves , Chabli Lila , Richard Pascale , Morel Pascal , Lot Florence , Tiberghien Pierre 
Published: September 2023

AbstractBackground and ObjectivesData from 21 years (2000–2020) of haemovigilance were used to assess human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) incidence rates in repeat blood donors and the occurrence of transfusion‐transmitted (TT) viral infections.Materials and MethodsBlood donors who converted for HIV, HCV or HBV markers within serial three‐year analysis periods were included. Epidemiological and virological data were retrieved from the national epidemiological donor database and were supplemented with information on blood components and the infection status of recipients of the previous negative donation (D.N‐1) of donors who seroconverted.ResultsIncidence rates declined from 1.27 to 0.35/100,000 person‐years for HIV, from 0.59 to 0.19 for HCV and from 1.66 to 0.18 for HBV. Risk factors and lookback for 232 HIV, 90 HCV and 74 HBV seroconversions were investigated. The main risk factor identified at post‐donation interview was having sex with men (47.8% of males) for HIV and a sexual risk for HCV (30.6%) and HBV (37.1%). The viral loads and sequences were retrospectively tested in 191 HIV, 74 HCV and 62 HBV D.N‐1 archived samples. Six (five HBV and one HIV‐1) were positive all low viral loads. Two recipients were infected by red blood cells from two HBV seroconverting donors before the introduction of HBV‐nucleic acid testing.ConclusionHIV, HCV and HBV incidence rates in blood donors declined over the two past decades in France. There is a very small risk of a blood component that tests negative entering the blood supply resulting in TT infections, especially after introduction of molecular assays in donor screening.
Development of a xylose-inducible promoter and riboswitch combination system for manipulating gene expression in
Fusobacterium nucleatum
Authors: G C Bibek , Zhou Peng , Naha Arindam , Gu Jianhua , Wu Chenggang 
Published: September 2023

ABSTRACT

Inducible gene expression systems are important for studying bacterial gene function, yet most exhibit leakage. In this study, we engineered a leakage-free hybrid system for precise gene expression controls in
Fusobacterium nucleatum
by integrating the xylose-inducible expression system with the theophylline-responsive riboswitch. This innovative method enables concurrent control of target gene expression at both transcription and translation initiation levels. Using luciferase and the indole-producing enzyme tryptophanase (TnaA) as reporters, we demonstrated that the hybrid system displays virtually no observable signal in the absence of inducers. We employed this system to express FtsX, a protein related to fusobacterial cytokinesis, in an
ftsX
mutant strain, unveiling a dose-dependent manner in FtsX production. Without inducers, cells form long filaments, while increasing FtsX levels by increasing inducer concentrations led to a gradual reduction in cell length until normal morphology was restored. Crucially, this system facilitated essential gene investigation, identifying the signal peptidase
lepB
gene as vital for
F. nucleatum
. LepB’s essentiality stems from depletion, affecting outer membrane biogenesis and cell division. This novel hybrid system holds the potential for advancing research on essential genes and accurate gene regulation in
F. nucleatum
.

IMPORTANCE

Fusobacterium nucleatum
, an anaerobic bacterium prevalent in the human oral cavity, is strongly linked to periodontitis and can colonize areas beyond the oral cavity, such as the placenta and gastrointestinal tract, causing adverse pregnancy outcomes and promoting colorectal cancer growth. Given
F. nucleatum
’s clinical significance, research is underway to develop targeted therapies to inhibit its growth or eradicate the bacterium specifically. Essential genes, crucial for bacterial survival, growth, and reproduction, are promising drug targets. A leak-free-inducible gene expression system is needed for studying these genes, enabling conditional gene knockouts and elucidating the importance of those essential genes. Our study identified
lepB
as the essential gene by first generating a conditional gene mutation in
F. nucleatum
. Combining a xylose-inducible system with a riboswitch facilitated the analysis of essential genes in
F. nucleatum
, paving the way for potential drug development targeting this bacterium for various clinical applications.
Sr-Isotopic Composition of Paleoocean Water 960 Ma Ago (according to the Data for the Nizhny Tunguska Formation of the Turukhansk Uplift, Siberian Platform)
Authors: Letnikova E. F. , Zhdanov A. A. , Ivanov A. V. , Maslov A. V. , Izokh A. E. , Letnikova A. F. , Soloshenko N. G. 
Published: September 2023

Abstract
The Sr-isotopic composition in carbonate rocks aged 980–920 Ma has been defined in only one place in the world, the Huainan Formation of the North China Platform, with the accumulation time limited by U–Pb data of detrital zircon grains. The upper part of the Nizhny Tunguska Formation in the Turukhansk uplift is found to contain altered volcanic rocks, as well as weathering crust products along them, such as poor bauxite and chamosite ores, among carbonate deposits. Based on U–Pb zircon isotope dating, the age of the volcanic rocks is 963 Ma. For the least altered limestones in the upper part of the Nizhny Tunguska Formation, the 87Sr/86Sr values were obtained equal to 0.70532–0.70578, which are close to those found in the rocks of the Huainan Formation. These data about the geochronological age of the studied limestones make it possible to obtain more accurate data, which allows refining the previously proposed configuration of the Sr-isotopic composition variation curve in the Early Neoproterozoic. The Nizhny Tunguska Formation compares favorably with the Huainan Formation in being the world’s only Early Neoproterozoic carbonate sequence that is correctly geochronologically substantiated (based on zircon from subsynchronous volcanic rocks).
Inferior vena cava diameter and risk of acute decompensated heart failure rehospitalisations
Authors: Sampath-Kumar Revathy , Ben-Yehuda Ori 
Published: September 2023

ObjectivesInferior vena cava (IVC) diameter may be a surrogate for volume status in acute decompensated heart failure (ADHF). The utility of IVC diameter measurement is under studied. The aim of this study was to assess the relationship between IVC diameter, clinical variables and ADHF rehospitalisations.MethodsRetrospective chart review of 200 patients admitted for ADHF from 2018 to 2019 with transthoracic echocardiogram during index hospitalisation. Charts were assessed for ADHF rehospitalisation within 1 year.ResultsThe median age was 64, 30.5% were female, and average left ventricular ejection fraction was 41%±20%. IVC diameter correlated to pulmonary arterial (PA) pressure (R=0.347, p<0.001) and body surface area (BSA) (R=0.424 p<0.001). IVC diameter corrected for BSA correlated to PA pressure (R=0.287, p<0.001) and log N-terminal B-type natriuretic peptide (NT-proBNP) (R=0.247, p≤0.01). Patients rehospitalised within 1 year had significantly greater mean IVC diameter compared with those not rehospitalised (p<0.001) while there was no difference in mean net weight lost during index hospitalisation or mean log NT-proBNP. Patients with IVC diameter greater than 2.07 cm had significantly increased ADHF rehospitalisation (85.6% vs 49.3%, log rank p<0.001) with HR 2.44 (95% CI 1.85 to 3.23, p<0.001). In multivariable Cox regression only IVC diameter (p<0.001), presence of tricuspid regurgitation (p=0.02) and NYHA class III/IV (p<0.001) independently predicted ADHF rehospitalisation within 1 year.ConclusionsIVC diameter is predictive of rehospitalisation in patients with ADHF and may identify patients in need of greater monitoring and diuresis.
Chronic beetroot juice supplementation attenuates neuromuscular fatigue etiology during simulated soccer match play
Authors: Daab Wael , Zghal Firas , Nassis George P. , Rebai Haithem , Moalla Wassim , Bouzid Mohamed Amine 
Published: September 2023

The aim of the present study was to assess the effect of beetroot juice supplementation (BEET) on neuromuscular fatigue etiology during simulated soccer match play. In a randomized, double-blind, crossover design, 13 soccer players completed the Loughborough Intermittent Shuttle Test (LIST). Players received either BEET (2×150 mL; ∼8 mmol/L nitrate) or placebo (PLA) for 7 days (6 days prior to the experimental session and on the day of trial, 2 h before LIST). Neuromuscular assessments were performed at baseline, 45 min (half time: HT), and 90 min (full time: FT) following LIST. Maximal voluntary contraction (MVC) and twitch responses, delivered through electrical femoral nerve stimulation, were used to assess peripheral (quadriceps resting twitch force Qtw,pot) and central fatigue (voluntary activation, VA). Compared with baseline, MVC Qtw,pot and VA values decreased in PLA and BEET conditions at HT and FT ( P < 0.05). Compared with PLA, the decrease in MVC and Qtw,pot was significantly attenuated with BEET at HT and FT ( P < 0.001). Likewise, BEET attenuated the decrease in VA at HT ( P < 0.001, d = 1.3) and FT ( P < 0.001, d = 1.5) compared with the PLA condition. Chronic beetroot juice supplementation attenuates neuromuscular fatigue development during simulated soccer matches, and this is due to both central and peripheral factors. Consequently, chronic beetroot may optimize physical performance.
O olhar como vetor do pensamento e do gozo
Authors: Prado Magaly Parreira do 
Published: September 2023

Em A Superindústria do Imaginário: como o capital transformou o olhar em trabalho e se apropriou de tudo que é visível, Eugênio Bucci se debruçou sobre o tema por um terço de século e propõe reconfigurar e exponenciar a imbricação da linguística e da psicanálise na comunicação – transformada em centro do capitalismo extrator de dados, circulando-os como mercadoria, sobretudo entre os propagandistas, influindo no fluxo da informação, tão cara em tempos de um espalhamento desmedido de fake news. Assim, o olhar, como um vórtice que atua em circuitos secretos, é um ato de linguagem. Afinal, em sociedades fantasiosas, o sujeito, como uma espécie de joguete, tem seu pensamento substituído pelo olhar para, ao final, encontrar o gozo.
Causal associations of 25-hydroxyvitamin D with functional gastrointestinal disorders: a two-sample Mendelian randomization study
Authors: Xu Senbao , Luo Qiuyan , He Jian , Chen Xiling , Li Simin , Bai Yang 
Published: September 2023

Abstract
Background
Previous observational studies have shown associations between vitamin Ds and FGIDS[Including irritable bowel syndrome(IBS) and functional dyspepsia(FD)]. However, the association is controversial and the causality remains unknown. In this study, two-sample MR was cited to explore the causal effect on FGIDS caused by vitamin D level and serum 25-hydroxyvitamin D.

Method
The GWASs of vitaminD and 25-hydroxyvitamin D, with 57–99 strongly related SNPs were all obtained from UK biobank. The GWASs of IBS and FD were obtained from FinnGen biobank with respectively 187,028 and 194,071 participants involved. Fixed-effect inverse variance weighted regression was used to evaluate causal estimates. Other statistical methods such as MR Egger, weighted median estimation, maximum likelihood estimation and penalty-weighted median estimation are also used to verify the accuracy of the main results.

Results
Measuring by the IVW method, our research indicated that no causal relationship was detected between vitamin D intake and Functional gastrointestinal disorders [IVW, OR(vitamin D-IBS) = 0.909, 95% CI 0.789–1.053, p = 0.2017); OR(vitamin D-FD) = 1.0662, 95% CI 0.9182–1.2380, p = 0.4000]. As for serum 25-hydroxyvitamin D, no causal relationship was detected on FD(IVW, OR(25-hydroxyvitamin D-FD) = 0.9635, 95% CI 0.8039–1.1546, p = 0.6869). Nevertheless, a negative causal relationship was revealed between 25-hydroxyvitamin D and IBS(IVW, OR(25-hydroxyvitamin D-IBS) = 0.832, 95% CI 0.696–0.995, p = 0.0436). Sensitive analysis supported the main findings but did not suggest bias due to pleiotropy.

Conclusions
Our Mendelian randomization analyses suggest a negative causal relationship between 25-hydroxyvitamin D and IBS. For each additional SD increase of genetically determined 25-hydroxyvitamin D levels, the risk of IBS decreased by 16.8%.
Anatomic versus non-anatomic resection for early-stage intrahepatic cholangiocarcinoma: a propensity score matching and stabilized inverse probability of treatment weighting analysis
Authors: Ke Qiao , Wang Lei , Lin Ziguo , Liu Hongzhi , Lou Jianying , Zheng Shuguo , Bi Xinyu , Wang Jianming , Guo Wei , Li Fuyu , Wang Jian , Zheng Yamin , Li Jingdong , Cheng Shi , Zhou Weiping , Liu Jingfeng , Zeng Yongyi 
Published: September 2023

Abstract
Background
Radical resection is still the most cost-effectiveness curative strategy for intrahepatic cholangiocarcinoma (ICC), but it remains controversial on the survival benefit of anatomic resection (AR). In this study, we sought to compare the oncologic outcomes between AR versus non-AR (NAR) as the primary treatment for early-stage ICC patients.

Methods
Data of ICC patients who underwent hepatectomy and staged at AJCC I were retrospectively collected from 12 hepatobiliary centers in China between Dec 2012 and Dec 2015. Propensity score matching (PSM) and stabilized inverse probability of treatment weighting (IPTW) analysis were performed to minimize the effect of potential confounders, and the perioperative and long-term outcomes between AR and NAR groups were compared.

Results
Two hundred seventy-eight ICC patients staged at AJCC I were eligible for this study, including 126 patients receiving AR and 152 patients receiving NAR. Compared to the NAR group, the AR group experienced more intraoperative blood loss before and after PSM or stabilized IPTW (all P > 0.05); AR group also experienced more intraoperative transfusion after stabilized IPTW (P > 0.05). In terms of disease-free survival (DFS) and overall survival (OS), no significant differences were observed between the two groups before and after PSM or stabilized IPTW (all P > 0.05). Multivariable Cox regression analyses found that AR was not an independent prognostic factor for either DFS or OS (all P > 0.05). Further analysis also showed that the survival benefit of AR was not found in any subgroup stratified by Child–Pugh grade (A or B), cirrhosis (presence or absence), tumor diameter (≤ 5 cm or > 5 cm) and pathological type (mass-forming or non-mass-forming) with all P > 0.05.

Conclusion
Surgical approach does not influence the prognosis of patients with stage I primary ICC, and NAR might be acceptable and oncological safety.
Oncoplastic breast conserving surgery versus standard breast conserving surgery for early and locally advanced breast cancer: a retrospective analysis from Sri Lanka
Authors: Wijesinghe Kanchana , Abeywickrama Thilanka , Chamara Yohan , De Silva Sumali , Tharshan Sebastianpillai , Jayarajah Umesh , De Silva Ajith 
Published: September 2023

Abstract
Background
Breast aesthetics is becoming increasingly important in breast cancer surgery due to changes in patient expectations and greater emphasis been placed on the psychosocial outcomes. Studies have shown no difference in local recurrence risk between mastectomy and breast conserving surgery (BCS) and also a higher overall survival rate after BCS. Breast preservation improves the quality of life substantially compared to mastectomy. Oncoplastic breast-conserving surgery (O-BCS) involves tumour excision whilst overcoming the limitations of standard breast conserving surgery (S-BCS) by allowing larger resection volumes, avoiding deformities with better aesthetic results. Our study aims to compare the oncosurgical and aesthetic outcomes of O-BCS versus S-BCS among women in Sri Lanka.

Methods
We conducted a retrospective study over a 4-year period including patients who underwent breast conservation surgery for primary non-metastatic breast cancer in two tertiary care units. We assessed outcomes in terms of re-excision rates, resection margin, complications and aesthetic outcomes using a Likert scale questionnaire to grade specific outcomes such as symmetry, volume, nipple position, scar visibility. Non-parametric tests were used for statistical analyses.

Results
Fifty-four and seventy-three patients underwent S-BCS and O-BCS respectively. The median specimen volume and the maximum tumour diameter were significantly higher in O-BCS [160(range:65–220); 4.2(range: 1.2–5.2)] compared to S-BCS [65(range:45–86); 2.4(range: 1.0-2.6)]. The median closest tumour margin was 16 mm (range:4-25 mm) in O-BCS while 6 mm (range:<1 – 12 mm) in S-BCS (p = 0.01). Close (< 1 mm) and positive margins needing re-excision were seen mostly in S-BCS. Superior aesthetic outcomes with statistical significant difference were reported in the O-BCS compared to S-BCS group with better symmetry, volume, nipple position and scar visibility. The re-excision rates were significantly lower in O-BCS group. There was no significant difference in the operative time and complications while the aesthetic outcomes were significantly superior in OBCS.

Conclusions
Overall, Level 2 perforator flap based reconstruction had superior aesthetic outcomes. O-BCS is safe and more aesthetically acceptable with no difference in oncological outcome and operative time. More consideration should be given to aesthetic parameters such as scar visibility, nipple position, breast volume and shape when considering the best surgical option for the patients.