bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bibliometric analysis of the Global Myelodysplastic Syndrome Study 1998-2022
Authors: Ma Junlan , Zhang Wei , Li Jiaojiao , Zhai Yan , Shen Li 
Published: April 2023

Abstract
Myelodysplastic syndromes (MDS) is a common myeloid malignancy with a high risk of transformation into acute leukaemia. In recent decades, the treatment of MDS has made great progress, and thousands of papers on MDS treatment-related research have been published. We conducted a bibliometric analysis of the literature on MDS and discussed collaboration between authors, countries and institutions, identifying research hotspots in the field of MDS. We searched the Web of Science (core collection) on the subject of "Myelodysplastic syndromes" or "Myelodysplastic syndromes" from January 1998 to September 2022, and analyzed the year of publication, research direction, country, institution, journal, distribution of authors, In addition, visual analysis was performed with CiteSpace, VOSviewer, Gephi, Charticulator, Scimago Graphica, and network graphs were generated to evaluate collaborations between different authors, countries, and institutions and the year of outbreak in keyword concentration, as well as the time zone map display of keywords. We searched 5218 articles from WOSCC as of September 29, 2022, and we found that the number of publications fluctuated from 1998 to 2008, remained relatively stable, and showed a slow upward trend in the following years. The most published countries are the United States, Germany and Italy, and the most cited country is Sweden, where the close collaborative research of the National House is crucial to progress in the field of MDS. Since 1998, hematopoietic stem cell transplantation and post-transplantation prognosis risk research has been a research hotspot, until 2009, the discovery of azacitidine and other demethylation drugs provided new treatment strategies in the field of MDS, more and more clinical trials are committed to finding new targeted drugs and exploring new prognostic risk factors for MDS, MDS treatment and prognosis have also received continuous attention. Bibliometric analysis of MDS-related research can help researchers and clinicians quickly understand the research hotspots and development trends in this field, provide researchers with new research angles, and provide ideas for the development of new treatment strategies.
Diagnostic Accuracy of Deep Learning in Medical Image Analysis - A Case Study Using Deep Burns
Authors: Abubakar Aliyu , Ajuji Mohammed , Turaki Abdulkadir Maigari 
Published: April 2023

Abstract
Purpose
Precise evaluation of burn depth is essential for determining the appropriate patient care and surgical requirements. This study aimed to examine a supervised machine learning approach that incorporates dynamic feature selection for differentiating between partial-thickness and full-thickness burns, utilizing deep learning patterns in digital images.
Method
Four deep learning models (VGG-16, ResNet-50, Xception, and EfficientNetV2L), along with two classifiers (Support Vector Machine and Fully Connected layer), were used to extract features from digital images of burn wounds, implementing dynamic feature selection during the training process. The models were trained using 3-fold cross-validation and tested on an unseen data split.
Results
The proposed method achieved high prediction accuracy, with the best performance achieved using EfficientNetV2L and SVM, yielding a specificity of 99.38%, sensitivity of 100.00%, precision of 99.35%, and an AUC value of 0.9969.
Conclusion
The results indicate that the proposed approach, which employs dynamic feature selection, holds potential for clinical effectiveness in objectively assessing burn depths. This technique can aid in informed decision-making regarding patient care and surgical intervention. However, further research is required to investigate its robustness in discriminating various skin wound depths.
Identification of a novel microRNA signature for prediction of clinical prognosis and drug sensitivity/resistance analysis in Hepatocellular Carcinoma
Authors: Lu Guoan , Li Xianbin 
Published: April 2023

Abstract
Prognostic stratification of hepatocellular carcinoma (HCC) remains challenging. It is found that microRNAs play a crucial role in pathogenesis of HCC. Our aims to construct a useful prognostic model based on miRNA signature to determine patient at high risk of HCC progression. Detailed information of miRNA were collected from The Cancer Genome Atlas (TCGA). We categorized HCC patients into two groups (training group and testing group) and identified the prognostic miRNA signature by univariate Cox regression analysis. A novel model based on miRNA was developed for HCC prognostic prediction. Then we apply ROC curve, KM curve and multivariate Cox regression analysis to assess the prognostic performance of the novel model. The results showed that AUC values based on ROC analysis of one, three and five-year overall survival (OS) for patients were 0.742, 0.723, 0.841, and 0.706, 0.653, 0.581, and 0.721, 0.702, 0.716 for training group, testing group and whole group, respectively. It indicated that the predictive performance of prognostic model was good. The nine-miRNA signature (miR-139, miR-125b-2, miR-125b-1, miR-4746, miR-1180, miR-592, miR-9-2, miR-9-1 and miR-9-3) could independently predict OS by using cox regression analysis method. The survival rate of patients in the high-risk group was lower than that in the low-risk group (all p < 0.001). In order to predict the potential biological functions of nine-miRNA signature, a total of 356 overlapping miRNA-target genes were analyzed by pathway enrichment analysis. In addition, the results showed that the DEGs mainly enriched in cancer-related pathways, including microRNAs in cancer, Longevity regulating pathway, AMPK signaling pathway, FoxO signaling pathway, Yersinia infection and T cell receptor signaling pathway. Function analysis of miRNAs showed that the nine miRNAs are related to various critical biological function modules and interact closely based on their functional similarity. Besides, most of miRNAs can promote sensitivity or induce resistance to some anti-cancer drugs.Taken together, these findings suggested that the nine-miRNA signature model could be conductive to the prognostic diagnosis and treatment of HCC.
Gestational diabetes mellitus and risk of dementia in late life: a prospective cohort study of United Kingdom Biobank
Authors: Wu Liting , Lai Fenghua , Chen Nan , Liu Yihao , Zeng Rui , Xu Changliu , Yang Xinlei , Li Yanbing , Xiao Haipeng , Cao Xiaopei 
Published: April 2023

Abstract
Although it has been demonstrated that gestational preeclampsia increased the risk of dementia, there is limited information regarding the relation between history of gestational diabetes mellitus (GDM) and the risk of late life dementia. In the present study, we analyzed the correlation using United Kingdom (UK) Biobank data. Propensity score-based fine stratification weighting and a Cox proportional hazards regression model were used and the risk of dementia was presented by weighted hazard ratio (HR) with 95% confidence intervals (CIs). A multi-state model was then used to analyze the impact of GDM on the progression of dementia from diseases, including type 2 diabetes (T2DM), hypertension, stroke, and myocardial infarction (MI). The study cohort consisted of 1,177 women who were previously exposed to GDM and 217,728 women who were not previously exposed to GDM. Overall, the adjusted dementia risk was approximately 1.67- to 2.02-fold higher for women exposed to previous GDM than those not exposed. In particular, GDM exposure increased the risk for dementia among women who experienced postpartum T2DM (HR = 1.92, 95% CI = 1.05–3.53), hypertension (HR = 2.00, 95% CI = 1.13–3.55), and MI (HR = 3.16, 95% CI = 1.28–7.75). However, for women without the above postpartum complications, the risk of late life dementia did not increase. Therefore, GDM is associated with an increased risk of all-cause dementia, which may be attenuated by preventive measures against postpartum T2DM, hypertension, and MI. Barriers and strategies for preventing postpartum dementia-risk complications should be further evaluated in future studies.
Formulation &amp; Evaluation of Polyherbal Antidiabetic Powder
Authors: Gawade Mahesh , Adlinge Akshay , Lipabe Vishal 
Published: April 2023

Abstract
Diabetes is a silent killer that causes significant economic damage in underdeveloped nations like India. To reduce the strain on a person's health and economy as well as the burden on society as a whole, better therapies must be developed with fewer side effects. The study's primary objective was to create a Evaluation of the polyherbal powder for diabetic mellitus based on organoleptic, rheological, physical, and phytochemical traits. The herbs used to make the polyherbal powder were annona squamosal, Trigonella foenum-graecum, Murraya koenigii, Aegle marmelos Correa, Mentha spicata Standardized methods were used to conduct the evaluations. The polyherbal powder's organoleptic characteristics were determined to be a dull brown colour, distinctive odour, astringent taste, and a reasonably fine texture. An examination of the phytochemicals revealed the presence of flavonoids, alkaloids, terpenoids, tannins, and carbs, glycosides, and steroids. The polyherbal powder had prolonged stability and good flow characteristics, according to physicochemical examination. Consequently, the polyherbal powder that may be used to treat diabetes mellitus was assessed.
Characterization of rumen microbiome and immune genes expression of crossbred beef steers with divergent residual feed intake phenotypes
Authors: Taiwo Godstime , Morenikeji Olanrewaju B. , Idowu Modoluwamu , Sidney Taylor , Adekunle Ajiboye , Cervantes Andres Pech , Peters Sunday O. , Ogunade Ibukun M. 
Published: April 2023

Abstract
We investigated whole blood and hepatic mRNA expressions of immune genes and rumen microbiome of crossbred beef steers with divergent residual feed intake phenotype to identify relevant biological processes underpinning feed efficiency in beef cattle. Low-RFI beef steers (n = 20; RFI = − 1.83 kg/d) and high-RFI beef steers (n = 20; RFI = + 2.12kg/d) were selected from a group of 108 growing crossbred beef steers (average BW = 282 ± 30.4 kg) fed a high-forage total mixed ration after a 70-d performance testing period. At the end of the 70-d testing period, liver biopsies and blood samples were collected for total RNA extraction and cDNA synthesis. Rumen fluid samples were also collected for analysis of the rumen microbial community. The mRNA expression of 84 genes related to innate and adaptive immunity was analyzed using pathway-focused PCR-based arrays. Differentially expressed genes were determined using P-value ≤ 0.05 and fold change (FC) ≥ 1.5 (in whole blood) or ≥ 2.0 (in liver). Gene ontology analysis of the differentially expressed genes revealed that pathways related to pattern recognition receptor activity, positive regulation of phagocytosis, positive regulation of vitamin metabolic process, vascular endothelial growth factor production, positive regulation of epithelial tube formation and T-helper cell differentiation were significantly enriched (FDR < 0.05) in low-RFI steers. In the rumen, the relative abundance of PeH15, Arthrobacter, Moryella, Weissella and Muribaculaceae was enriched in low-RFI steers, while Methanobrevibacter, Bacteroidales_BS11_gut_group, Bacteroides and Clostridium_sensu_stricto_1 were reduced. In conclusion, our study found that low-RFI beef steers exhibit increased mRNA expression of genes related to immune cell functions in whole blood and liver tissues, specifically those involved in pathogen recognition and phagocytosis regulation. Additionally, these low-RFI steers showed differences in relative abundance of some microbial taxa which may partially account for their improved feed efficiency compared to high-RFI steers.
Genotype by environment interaction of bread wheat genotypes under timely and late sown planting conditions in central zone of India
Authors: Khare Vikrant , Shukla R. S. , Pandey Suneeta , Singh S. K. 
Published: April 2023

Abstract

The purpose of this study was to ascertain the genotype by environment interaction (GEI) of heat tolerance wheat genotypes. The objective was to inspect the stable wheat genotype for timely and late sown planting condition in central zone of India. For that total of 20 wheat genotypes, including two parents and three commercial checks, were tested across 6 (timely sown) + 4 (late sown) environments at Jabalpur, Narmadapuram, and Sagar district of Madhya Pradesh state of India in 2019-20 and 2021-21. The per plant yield and grain filling rate data were considered to perform univariate and multivariate stability analysis. Our result revealed that environment, genotype, and GEI effects were significant (P < 0.001) across all the environments and individual environmental conditions. The greater performing along with high stability for grain yield JW3288, L8 and L13 while for grain filling rate L11, L13 and L11 genotypes were identified in timely sown, late sown and across all the environments, respectively. In order to find stable and high-performing genotypes, the GEI accompanied by several models but AMMI and GGE models were further effective and accurate than the linear regression model. In conclusion according to univariate and multivariate stability analysis L13 was the utmost genotype across all the environments therefore, it might be used in future breeding programs although, Jabalpur was recognized as the most discriminating and representative environments across all the environments.
Inflammatory Response Modulation by Low-Dose Anti-inflammatory
Drugs Treatment in an In Vitro Osteoarthritis Cellular Model
Authors: Iuliano Marco , Santilli Valter , Mineo Andrea , Paoloni Marco , Rosa Paolo , Mangino Giorgio , Romeo Giovanna 
Published: April 2023

Background:
Low-dose-medicine is based on the administration of low doses
of biological regulators to restore the immunologic balance altered in the disease. Cytokines
are pivotal regulators of cellular and tissue functions and impaired crosstalk, due
to an imbalance between specific cytokines, it is fundamental in acute inflammation and
diseases correlated to low-grade chronic inflammation. Osteoarthritis is the most prevalent
arthritic disease and a leading cause of disability. In the treatment of muscle- skeletal
pathologies, the therapeutic integration of conventional medicine with homotoxicology,
or low-dose-medicine appears to be beneficial.

Objective:
This study aims to get more insights into the role of inflammatory cytokines
and chemokines during the development of osteoarthritis and to evaluate a possible blocking
strategy using anti-inflammatory molecules, we resort to an in vitro experimental
model using an established human chondrosarcoma cell line that underwent to a well
known pro-inflammatory stimulus as bacterial lipopolysaccharide.

Methods:
We tested the production of inflammatory-related cytokines and chemokines,
and the efficacy of low-dose (LD) administration of anti-inflammatory compounds, namely
IL-10 and anti-IL-1, to block inflammatory cellular pathways.

Results:
Following an inflammatory insult, chondrocytes upregulated the expression of
several pro-inflammatory cyto-/chemokines and this induction could be counteracted by
LD IL-10 and anti-IL-1. We reported that these effects could be ascribed to an interfering
effect of LD drugs with the NF-κB signaling.

Conclusion:
Our results provided a good indication that LD drugs can be effective in inhibiting
the inflammatory response in chondrocytes opening the way to new therapies for
the treatment of diseases such as osteoarthritis.
Assessment of Cytotoxic/Antitumour Potential and in silico Study of Salazinic Acid
Isolated from Parmotrema concurrens
Authors: da Conceição de Lira Maria Aparecida , da Silva Marllyn Marques , Rocha Tamiris Alves , de Moura Danielle Feijó , Santos Costa Erick Caique , dos Santos Maia Mayara , Scotti Luciana , Scotti Marcus Tullius , de Lourdes Lacerda Buril Maria , Pereira Eugênia Cristina , de Aguiar Júnior Francisco Carlos Amanajás , de Britto Lira Nogueira Mariane Cajubá , da Silva Santos Noemia Pereira , da Silva Falcão Emerson Peter , de Melo Sebastião José 
Published: April 2023

Introduction:
Despite numerous scientific advances, cancer continues to be one of the main causes of death
in the world. This situation has driven the search for promising molecules. Lichen substances have been widely described
for their pharmacological potential.

Objective:
The present study evaluated the antitumour potential of a depsidone isolated from Parmotrema concurrens–
salazinic acid (SAL) – through in vitro, in vivo and in silico studies.

Methods:
The molecule was isolated from the acetonic extract of the lichen and recrystallized in acetone. The macrophage
J774, sarcoma-180 and MDA-MB-231 cell lines were used for the MTT cytotoxicity assay. The antitumor assay
used a murine model (Swiss albino mice) with sarcoma-180. The animals were treated for seven consecutive days with
doses of SAL (25 and 50 mg/kg) and 5-fluorouracil (20 mg/kg).

Results:
Its purity was determined using high-performance liquid chromatography (94%), and its structure was confirmed
by H1 and C13 nuclear magnetic resonance. SAL was not considered toxic to cancer cell lines, showing cell
viability rates of 79.49 ± 4.15% and 86.88 ± 1.02% for sarcoma-180 and MDA-MB-231, respectively. The tumour
inhibition rate was greater than 80% in the animals treated with SAL and 65% for those that received 5-fluorouracil.
Simulations of molecular dynamics to estimate the flexibility of the interactions between human thymidylate synthase
and derivatives of SAL and 5-fluorouracil revealed that SAL exhibited greater enzymatic interaction capacity, with
highly favourable energy, compared to 5-fluorouracil.

Conclusion:
The present results demonstrate the potential of salazinic acid as a tumour inhibition agent.
Prevalence and Cause of Enamel Hypoplasia in Primary Teeth among 1-year-old Japanese Children
Authors: Miyamoto Akane , Minagawa Kumiko , Nohno Kaname , Kaneko Noboru , Ichikawa Yu , Hoshino Takashi , Ito Harue , Yoshihara Akihiro 
Published: April 2023

Objective:
In this study, the prevalence and underlying cause of enamel hypoplasia in one-year-old children were examined. Additionally, to determine the association between enamel hypoplasia and exposure to sunlight based on the frequency of the mother’s outings during pregnancy.

Methods:
A total of 273 kids (139 boys) were involved in this study. Based on questionnaires surveys to mothers, oral examinations, and photographs of children's mouths, we looked at the connection between the circumstances of pregnancy and the occurrence of enamel hypoplasia. The presence of enamel hypoplasia was assessed using logistic regression analysis differed among the results of the questionnaire.

Results:
The prevalence of enamel hypoplasia was 38.5%. In the medium and low groups, enamel hypoplasia was more common than in the high group at the birth month of the child (adjusted odds ratio: 3.36, 95% confidence interval: 1.65–6.85, p = 0.001 vs. adjusted odds ratio: 3.76, 95% confidence interval: 1.89–7.48, p < 0.001, respectively). Enamel hypoplasia was knowingly higher among the children whose mothers went out less than 1–2 times a week during pregnancy (adjusted odds ratio: 2.41, 95% confidence interval: 1.04–5.61, p = 0.041). Enamel hypoplasia was significantly more prevalent when mothers used painkillers during pregnancy (adjusted odds ratio: 5.45, 95% Confidence interval: 1.74–17.08, p = 0.004).

Conclusion:
It was suggested that the use of antipyretic analgesics during pregnancy and frequency of outings and variations in sunshine hours according to the child's birth month could affect the occurrence of enamel hypoplasia.
Case Report: Acute Retinal Necrosis following Unexpanded Gas Tamponade Acute Retinal Necrosis following Unexpanded Gas Tamponade
Authors: Sezgin Hamza , Herrmann Wolfgang , Prahs Philipp , Galal Ahmed 
Published: April 2023

Purpose:
To present 2 cases of acute non-hemorrhagic retinal necrosis occurring following pars plana vitrectomy for epiretinal membrane peeling following the use of unexpanded gas tamponade.

Case Report:
Two eyes (both right eyes) of 2 healthy females presented with decreased visual acuity due to Epiretinal membrane, and they underwent 25G vitrectomy and membrane peeling. The vitrectomy operation was uneventful and was concluded with the insertion of 20% sulfur hexafluoride (SF6) tamponade. Both eyes were pseudophakic and had no other ocular or systemic pathology. Patients were controlled 24 hours following the surgery and both presented with normal anterior segment examination and normal intraocular pressure. Both patients complained of central scotoma and both had very difficult light perception. Fundus examination showed devitalized macula structures in the presence of gas bubbles occupying almost 100% of the vitreous cavity. OCT examination done a few days after surgery revealed necrotic retinal tissue. Four weeks later, both eyes had light perception vision, and devitalization of the central macula and spicule-like pigment changes in the midperiphery of the retinal in both eyes.

Conclusion:
We present 2 cases of blindness after vitrectomy with ILM peeling combined with 20% SF6 Intraocular tamponade. This severe complication was not related to elevated intraocular pressure due to gas expansion.
Performance of a Silicone Hydrogel Daily Disposable Contact Lens among Wearers with Lens-related Dryness
Authors: Reindel William , Steffen Robert , Mosehauer Gary , Schafer Jeffery , Rah Marjorie , Shahidi Ayda , Proskin Howard 
Published: April 2023

Background:
Addressing contact lens dryness continues to be a development goal of contact lens (CL) manufacturers.

Objective:
The objective of this study is to evaluate the clinical performance of kalifilcon A, a daily disposable silicone hydrogel (SiHy) CL, in subjects that experience dryness with their habitual planned-replacement SiHy CLs relative to a non-dry subgroup.

Methods:
A cohort of adapted planned-replacement SiHy CL wearers wore kalifilcon A lenses for at least 8 hours daily over two weeks. After one week of lens wear, subjects completed a survey regarding their lens wearing experience with respect to comfort and vision. Subsequently, subjects visited the clinics for the 2-week visit, during which the investigators completed a slit lamp exam and questionnaire regarding lens performance.

Results:
The evaluation included 180 subjects experiencing CL dryness with their habitual SiHy lenses and 213 subjects that did not. Both subgroups largely agreed with all comfort and vision attribute statements, and the dryness subgroup expressed higher levels of agreement with most comfort-related statements. Among habitual rewetting drop users, 73.9% in the dryness subgroup and 73.1% in the non-dry subgroup used drops less frequently while wearing kalifilcon A lenses. Investigators found no > Grade 2 slit-lamp findings, nor differences between subgroups. Neither subgroup showed a change in ratings between visits, except for a significantly higher proportion of improvers in the non-dry subgroup for upper lid tarsal conjunctival abnormalities.

Conclusion:
The kalifilcon A lens performed well among habitual planned-replacement SiHy CLs wearers. Its unique chemistry can provide a more satisfying wear experience for SiHy lens wearers experiencing CL dryness.