bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selenium nanoparticles improve nickel‐induced testosterone synthesis disturbance by down‐regulating miR‐708‐5p/p38 MAPK pathway in Leydig cells
Authors: Wang Shuang , Gu Xueyan , Ma Jianhua , Gu Zhangyu , Zhang Rui , Li Ruifen , Bai Jun , Li Peng , Wei Linyu , Ye Yixing , Wang Yan , Zhang Li , Su Li , Liang Changhao 
Published: May 2023

AbstractThe present study was designed to investigate the role of miR‐708‐5p/p38 mitogen‐activated protein kinase (MAPK) pathway during the mechanism of selenium nanoparticles (Nano‐Se) against nickel (Ni)‐induced testosterone synthesis disorder in rat Leydig cells. We conducted all procedures based on in vitro culture of rat primary Leydig cells. After treating Leydig cells with Nano‐Se and NiSO4 alone or in combination for 24 h, we determined the cell viability, reactive oxygen species (ROS) levels, testosterone production, and the protein expression of key enzymes involved in testosterone biosynthesis: steroidogenic acute regulatory (StAR) and cytochrome P450 cholesterol side chain cleavage enzyme (CYP11A1). The results indicated that Nano‐Se antagonized cytotoxicity and eliminated ROS generation induced by NiSO4, suppressed p38 MAPK protein phosphorylation and reduced miR‐708‐5p expression. Importantly, we found that Nano‐Se upregulated the expression of testosterone synthase and increased testosterone production in Leydig cells. Furthermore, we investigated the effects of p38 MAPK and miR‐708‐5p using their specific inhibitor during Nano‐Se against Ni‐induced testosterone synthesis disorder. The results showed that Ni‐inhibited testosterone secretion was alleviated by Nano‐Se co‐treatment with p38 MAPK specific inhibitor SB203580 and miR‐708‐5p inhibitor, respectively. In conclusion, these findings suggested Nano‐Se could inhibit miR‐708‐5p/p38 MAPK pathway, and up‐regulate the key enzymes protein expression for testosterone synthesis, thereby antagonizing Ni‐induced disorder of testosterone synthesis in Leydig cells.
Whole genome resequencing and phenotyping of MAGIC population for high resolution mapping of drought tolerance in chickpea
Authors: Thudi Mahendar , Samineni Srinivasan , Li Wenhao , Boer Martin P. , Roorkiwal Manish , Yang Zuoquan , Ladejobi Funmi , Zheng Chaozhi , Chitikineni Annapurna , Nayak Sourav , He Zhang , Valluri Vinod , Bajaj Prasad , Khan Aamir W. , Gaur Pooran M. , van Eeuwijk Fred , Mott Richard , Xin Liu , Varshney Rajeev K. 
Published: May 2023

AbstractTerminal drought is one of the major constraints to crop production in chickpea (Cicer arietinum L.). In order to map drought tolerance related traits at high resolution, we sequenced multi‐parent advanced generation intercross (MAGIC) population using whole genome resequencing approach and phenotyped it under drought stress environments for two consecutive years (2013–14 and 2014–15). A total of 52.02 billion clean reads containing 4.67 TB clean data were generated on the 1136 MAGIC lines and eight parental lines. Alignment of clean data on to the reference genome enabled identification of a total, 932,172 of SNPs, 35,973 insertions, and 35,726 deletions among the parental lines. A high‐density genetic map was constructed using 57,180 SNPs spanning a map distance of 1606.69 cM. Using compressed mixed linear model, genome‐wide association study (GWAS) enabled us to identify 737 markers significantly associated with days to 50% flowering, days to maturity, plant height, 100 seed weight, biomass, and harvest index. In addition to the GWAS approach, an identity‐by‐descent (IBD)‐based mixed model approach was used to map quantitative trait loci (QTLs). The IBD‐based mixed model approach detected major QTLs that were comparable to those from the GWAS analysis as well as some exclusive QTLs with smaller effects. The candidate genes like FRIGIDA and CaTIFY4b can be used for enhancing drought tolerance in chickpea. The genomic resources, genetic map, marker‐trait associations, and QTLs identified in the study are valuable resources for the chickpea community for developing climate resilient chickpeas.
Evaluating the accuracy of a six‐lead smartphone‐based electrocardiographic device compared with standard electrocardiography in brachymorphic dogs
Authors: Romito Giovanni , Castagna Prisca , Sabetti Maria Chiara , Ablondi Michela , Cipone Mario 
Published: May 2023

AbstractBackgroundNo previous study has evaluated the accuracy of a six‐lead smartphone‐based electrocardiographic (s‐ECG) device in dogs.MethodsThis was a prospective multicentre study. Patients referred for cardiologic consultation were selected. In each patient, a 30‐second electrocardiogram was simultaneously acquired with a novel six‐lead s‐ECG and a standard six‐lead ECG machine (st‐ECG). A board‐certified cardiologist evaluated each recording. Nineteen ECG variables, including heart rate and rhythm, as well as quantitative and qualitative features of waves, segments and intervals, were analysed. Agreement between s‐ECG and st‐ECG was evaluated using Cohen's kappa coefficient and the Bland–Altman test.ResultsSeventy‐five dogs were enrolled, and 140 ECG tracings were analysed. There was perfect agreement between the two methodologies for heart rate and rhythm classification, both in dogs with sinus rhythm and those with pathological rhythms. Although some disagreement was found when comparing measurements of quantitative variables obtained with the s‐ECG and the st‐ECG, none of the differences was of clinical relevance.LimitationsThe sample size was limited, and the interobserver variability was not analysed.ConclusionThe six‐lead s‐ECG studied herein is comparable to the st‐ECG for heart rate and rhythm assessment, and seems clinically acceptable for the interpretation of waves, segments and intervals in dogs.
Time‐dependent coupled‐cluster theory
Authors: Sverdrup Ofstad Benedicte , Aurbakken Einar , Sigmundson Schøyen Øyvind , Kristiansen Håkon Emil , Kvaal Simen , Pedersen Thomas Bondo 
Published: May 2023

AbstractRecent years have witnessed an increasing interest in time‐dependent coupled‐cluster (TDCC) theory for simulating laser‐driven electronic dynamics in atoms and molecules, and for simulating molecular vibrational dynamics. Starting from the time‐dependent bivariational principle, we review different flavors of single‐reference TDCC theory with either orthonormal static, orthonormal time‐dependent, or biorthonormal time‐dependent spin orbitals. The time‐dependent extension of equation‐of‐motion coupled‐cluster theory is also discussed, along with the applications of TDCC methods to the calculation of linear absorption spectra, linear and low‐order nonlinear response functions, highly nonlinear high harmonic generation spectra and ionization dynamics. In addition, the role of TDCC theory in finite‐temperature many‐body quantum mechanics is briefly described along with a few other application areas.This article is categorized under:
Electronic Structure Theory > Ab Initio Electronic Structure Methods
Theoretical and Physical Chemistry > Spectroscopy
Software > Simulation Methods
Motion through a viscous liquid sphere enclosed by a solid core embedded into a Brinkman medium
Authors: Sakthivel Shyamala , Shukla Pankaj 
Published: May 2023

AbstractThe flow around a solid spherical particle encased in a Newtonian liquid sphere and immersed in a couple stress fluid medium is studied. The problem is expressed by using the Brinkman and Stokes equations, which describe both the flow outside and inside the liquid sphere, respectively. The Gegenbauer polynomials and modified Bessel function are used to express the stream function solution for the internal and external regions. An analytical determination for the flow field in terms of stream function is examined by wielding the method of separation of variables. The drag force on a solid spherical particle placed in a permeable region is calculated. On the drag coefficient, the effects of the permeability κ, the viscosity ratio γ2, and the couple stress parameter λ are investigated. Corresponding dependencies (such as the permeability parameter, couple stress parameter, viscosity ratio, and separation parameter) are graphically represented and discussed. The findings shows when the separation parameter is increased the drag coefficient gradually increases, it refers to a sphere surface with a high level of flow resistance. Passages to the limits are used to describe known specific cases. The present study is essentially significant in the course through a layer developed by penetrable particles and has very important and persuasive applications both in nature and innovation, with various potential outcomes. Thus, the discoveries of this article are comprehensively pertinent to the investigation of the flow of permeable liquids past spherical permeable rocks, aloxite materials, sand beds, earthen soil, petrol supply rocks, and so forth. The present application will support in planning a productive bearing framework.
Postoperative thyroglobulin as a yard-stick for radioiodine therapy: decision tree analysis in a European multicenter series of 1317 patients with differentiated thyroid cancer
Authors: Giovanella Luca , Milan Lisa , Roll Wolfgang , Weber Manuel , Schenke Simone , Kreissl Michael , Vrachimis Alexis , Pabst Kim , Murat Tuncel , Petranovic Ovcaricek Petra , Riemann Burkhard , Ceriani Luca , Campenni Alfredo , Görges Rainer 
Published: May 2023

AbstractPurposeAn accurate postoperative assessment is pivotal to inform postoperative131I treatment in patients with differentiated thyroid cancer (DTC). We developed a predictive model for post-treatment whole-body scintigraphy (PT-WBS) results (as a proxy for persistent disease) by adopting a decision tree model.MethodsAge, sex, histology, T stage, N stage, risk classes, remnant estimation, TSH, and Tg were identified as potential predictors and were put into regression algorithm (conditional inference tree, ctree) to develop a risk stratification model for predicting the presence of metastases in PT-WBS.ResultsThe lymph node (N) stage identified a partition of the population into two subgroups (N-positive vs N-negative). Among N-positive patients, a Tg value  > 23.3 ng/mL conferred a 83% probability to have metastatic disease compared to those with lower Tg values. Additionally, N-negative patients were further substratified in three subgroups with different risk rates according to their Tg values. The model remained stable and reproducible in the iterative process of cross validation.ConclusionsWe developed a simple and robust decision tree model able to provide reliable informations on the probability of persistent/metastatic DTC after surgery. These information may guide post-surgery131I administration and select patients requiring curative rather than adjuvant131I therapy schedules.
Study of the wavelength dependence of the absorption function of diesel soot by LII modeling integrating the effect of multiple scattering within aggregates
Authors: Lemaire Romain , Menanteau Sébastien 
Published: May 2023

AbstractThis work represents a first attempt at proposing a refined LII model suitable for simulating signals collected with excitation wavelengths (EWs) of 266, 355, 532 and 1064 nm. In this context, we implemented a comprehensive version of laser-irradiated soot heat and mass balance equations integrating terms representing the saturation of linear, single- and multi-photon absorption processes, cooling by sublimation, conduction, radiation and thermionic emission, as well as mechanisms depicting soot oxidation and annealing, non-thermal photodesorption of carbon clusters and corrective factors accounting for the shielding effect and multiple scattering (MS) within aggregates. This simulation tool was fully parameterized, by coupling design of experiments with a genetic algorithm-based solver, against data collected at different heights above the burner (HAB) in a diesel spray flame. This allowed to assess values of different model parameters involved in absorption and sublimation terms, which, to date, have never been reported in LII modeling studies for UV EWs (e.g., multi-photon absorption cross sections for C2 photodesorption and saturation coefficients for linear- and multi-photon absorption). The simulation work proposed herein then enabled to infer information regarding the evolution of the absorption function of soot ($$E\left(m,\lambda \right)$$

E

m
,
λ

). $$E\left(m,266\right)$$

E

m
,
266

, $$E(m,355)$$

E
(
m
,
355
)

and $$E(m,532)$$

E
(
m
,
532
)

ranging from 0.25 to 0.51, 0.20 to 0.38 and 0.18 to 0.30, respectively, were notably estimated as a function of the HAB. Alternatively, values ranging from 0.31 to 0.53, 0.26 to 0.44 and 0.22 to 0.38 were assessed while neglecting the effect of MS and provided constant $$E(m,266)$$

E
(
m
,
266
)

/$$E(m,1064)$$

E
(
m
,
1064
)

, $$E(m,355)$$

E
(
m
,
355
)

/$$E(m,1064)$$

E
(
m
,
1064
)

and $$E(m,532)$$

E
(
m
,
532
)

/$$E(m,1064)$$

E
(
m
,
1064
)

ratios of 1.4, 1.2 and 1.0, regardless of the HAB. In addition, the obtained results showed that the wavelength dependence of the soot absorption function was quite negligible for EWs higher than 532 nm, irrespective of whether the effect of MS was considered or neglected. On the other hand, aggregate properties were proven to substantially influence the $$E(m,\lambda )$$

E
(
m
,
λ
)

/$$E(m,1064)$$

E
(
m
,
1064
)

ratios for decreasing $$\lambda$$
λ
and increasing HAB, thus illustrating the significant effect of aggregation on the optical properties of soot. Finally, the results issued from the different analyses we conducted on the diesel flame investigated in this paper led to the conclusion that values of $$E\left(m,\lambda \right),$$

E

m
,
λ

,

falling within the 0.38 ± 0.15 and 0.29 ± 0.11 range at 266 and 355 nm, versus 0.25 ± 0.09 at 532 and 1064 nm could be considered as suitable for simulating LII signals of progressively aging aggregated particles.
Dietary supplements increase the risk of excessive micronutrient intakes in Danish children
Authors: Christensen Camilla , Matthiessen Jeppe , Fagt Sisse , Biltoft-Jensen Anja 
Published: May 2023

Abstract
Purpose
Dietary supplement use is common in Northern Europe. Many dietary supplements contain 100% of nutrient reference values (NRV) of micronutrients. This study investigates the contribution of dietary supplements to micronutrient intake, the prevalence of excess intake of micronutrients, and parental characteristics of dietary supplement use in Danish children.

Methods
Data on 499 4–10-year-old children from the Danish National Survey of Diet and Physical Activity 2011–2013 were analysed using non-parametric statistics to compare micronutrient intake from the diet and dietary supplements to the reference intake (RI), and to the tolerable Upper Intake Level (UL) for users and non-users of dietary supplements. Furthermore, characteristics of the parents of users and non-users of dietary supplements were examined by logistic regression analysis.

Results
Sixty-four percent of the children were dietary supplement users. Multivitamin-mineral supplements were the most frequently used type of supplement (60%). Children of never-smokers were more likely to use supplements than children of smokers. Users had significantly higher total intakes of 15 micronutrients compared to non-users. Intakes of vitamin A, zinc, and iodine from the diet alone exceeded ULs in 12–30% of the children. Use of dietary supplements gave rise to 21–73% of children in exceedance of the aforementioned three ULs as well as the UL for iron (6–45%).

Conclusion
Dietary supplement use was common among 4–10-year-old Danish children and resulted in a considerable proportion of users exceeding the ULs for vitamin A, zinc, iodine, and iron. The long-term health consequences of exceeding these ULs for children are unknown.
Termination of amnesiac flooding
Authors: Hussak Walter , Trehan Amitabh 
Published: May 2023

AbstractWe consider a stateless ‘amnesiac’ variant of the stateful distributed network flooding algorithm, expanding on our conference papers [PODC’19, STACS’20]. Flooding begins with a set of source ‘initial’ nodesIseeking to broadcast a messageMin rounds, in a network represented by an undirected graph (G, E) with set of nodesGand edgesE. In every round, nodes withMsendMto all neighbours from which they did not receiveMin the previous round. Nodes do not remember earlier rounds, raising the possibility thatMcirculates indefinitely. Stateful flooding overcomes this by nodes recording every message circulated and ignoringMif received again. This overhead was assumed to be necessary. We show that almost optimal broadcast can still be achieved without this overhead. We prove that amnesiac flooding terminates on every finite graph and derive sharp bounds for termination times. Define (G, E) to beI-bipartiteif the quotient graph, contracting all nodes inIto a single node, is bipartite. We prove that, ifdis the diameter ande(I) the eccentricity of the setI, flooding terminates ine(I) rounds if (G, E) is I-bipartite andjrounds withe(I) <j$$\le $$≤$$e(I)+d+1 \le 2d +1$$e(I)+d+1≤2d+1if (G, E) is non I-bipartite. The separation in the termination times can be used for distributed discovery of topologies/distances in graphs. Termination is guaranteed if edges are lost during flooding but not, in general, if there is a delay at an edge. However, the cases of single-edge fixed delays of duration$$\tau $$τrounds in single-source bipartite graphs terminate by round$$2d+\tau -1$$2d+τ-1, and all cases of multiple-edge fixed delays in multiple-source cycles terminate.
Fluid management and vasopressor use during colorectal surgery: the search for the optimal balance
Authors: Huisman Daitlin E. , Bootsma Boukje T. , Ingwersen Erik W. , Reudink Muriël , Slooter Gerrit D. , Stens Jurre , Daams Freek ,   the LekCheck Study group, Roumen Rudi M. H. M. H. , van Rooijen Stefanus J. , Bleeker Wim , Stassen Laurents P. S. , Jongen Audrey , Feo Carlo V. , Targa Simone , Komen Niels , Kroon Hidde M. , Sammour Tarik , Lagae Emmanuel A. G. L. , Talsma Aalbert K. , Wegdam Johannes A. , de Vries Reilingh Tammo S. , van Wely Bob , van Hoogstraten Marie J. , Sonneveld Dirk J. A. , Verdaasdonk Emiel G. G. 
Published: May 2023

Abstract
Background
Although it is known that excessive intraoperative fluid and vasopressor agents are detrimental for anastomotic healing, optimal anesthesiology protocols for colorectal surgery are currently lacking.

Objective
To scrutinize the current hemodynamic practice and vasopressor use and their relation to colorectal anastomotic leakage.

Design
A secondary analysis of a previously published prospective observational study: the LekCheck study.

Study setting
Adult patients undergoing a colorectal resection with the creation of a primary anastomosis.

Outcome measures
Colorectal anastomotic leakage (CAL) within 30 days postoperatively, hospital length of stay and 30-day mortality.

Results
Of the 1548 patients, 579 (37%) received vasopressor agents during surgery. Of these, 201 were treated with solely noradrenaline, 349 were treated with phenylephrine, and 29 received ephedrine. CAL rate significantly differed between the patients receiving vasopressor agents during surgery compared to patients without (11.8% vs 6.3%, p < 0.001). CAL was significantly higher in the group receiving phenylephrine compared to noradrenaline (14.3% vs 6%, p < 0.001). Vasopressor agents were used more often in patients treated with Goal Directed Therapy (47% vs 34.6%, p < 0.001). There was a higher mortality rate in patients with vasopressors compared to the group without (2.8% vs 0.4%, p = 0.01, OR 3.8). Mortality was higher in the noradrenaline group compared to the phenylephrine and those without vasopressors (5% vs. 0.4% and 1.7%, respectively, p < 0.001). In multivariable analysis, patients with intraoperative vasopressor agents had an increased risk to develop CAL (OR 2.1, CI 1.3–3.2, p = 0.001).

Conclusion
The present study contributes to the evidence that intraoperative use of vasopressor agents is associated with a higher rate of CAL. This study helps to create awareness on the (necessity to) use of vasopressor agents in colorectal surgery patients in striving for successful anastomotic wound healing. Future research will be required to balance vasopressor agent dosage in view of colorectal anastomotic leakage.
Non-oral continuous drug delivery based therapies and sleep dysfunction in Parkinson’s disease
Authors: Tall P. , Qamar M. A. , Batzu L. , Leta V. , Falup-Pecurariu C. , Ray Chaudhuri K. 
Published: May 2023

AbstractContinuous drug delivery (CDD) has emerged as a feasible and pragmatic therapeutic option for dopamine replacement therapy in advanced Parkinson’s disease (PD). CDD aims to mimic the physiological tonic dopamine release from striatal dopaminergic neurons and thus reduces the severity and duration of motor and non-motor fluctuations partly related to pulsatile levodopa stimulation. Non-motor symptoms and fluctuations are ubiquitous in PD and include sleep dysfunction, a problem that occurs in over 90% of PD patients across all stages, from prodromal to palliative. In this review, we discuss the currently available and in development non-oral dopaminergic CDD strategies with a focus on their efficacy in the treatment of the burdensome sleep dysfunction in PD.
Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs
Authors: López-García Sergio , Rodríguez-Lozano Francisco J. , Sanz José Luis , Forner Leopoldo , Pecci-Lloret María Pilar , Lozano Adrián , Murcia Laura , Sánchez-Bautista Sonia , Oñate-Sánchez Ricardo E. 
Published: May 2023

Abstract
Objectives
To assess the cytocompatibility and bioactive potential of the new calcium silicate-based cement Ceraputty on human periodontal ligament stem cells (hPDLSCs) compared to Biodentine and Endosequence BC root repair material (ERRM).

Materials and methods
hPDLSCs were isolated from extracted third molars from healthy donors. Standardized sample discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, osteo/cemento/odontogenic marker expression via RT-qPCR, and cell calcified nodule formation via Alizarin Red S staining. hPDLSCs cultured in unconditioned or osteogenic media were used as negative and positive control groups, respectively. Statistical analysis was performed using one-way ANOVA or two-way ANOVA and Tukey’s post hoc test. Statistical significance was established at p < 0.05.

Results
The highest Ca2+ peak was detected from Biodentine samples, followed by ERRM and Ceraputty. hPDLSC viability was significantly reduced in Ceraputty samples (p < 0.001), while 1:2 and 1:4 Biodentine and ERRM samples similar results to that of the negative control (p > 0.05). Biodentine and ERRM exhibited an upregulation of at least one cemento/odonto/osteogenic marker compared to the negative and positive control groups. Cells cultured with Biodentine produced a significantly higher calcified nodule formation than ERRM and Ceraputty (p < 0.001), which were also higher than the control groups (p < 0.001).

Conclusion
Ceraputty evidenced a reduced cytocompatibility towards hPDLSCs on its lowest dilutions compared to the other tested cements and the control group. Biodentine and ERRM promoted a significantly higher mineralization and osteo/cementogenic marker expression on hPDLSCs compared with Ceraputty. Further studies are necessary to verify the biological properties of this new material and its adequacy as a retrograde filling material.

Clinical relevance
This is the first study to elucidate the adequate biological properties of Ceraputty for its use as a retrograde filling material.