bluuebunny/crossref_metadata_2025_split huggingface dataset:
การพัฒนารูปแบบการเรียนการสอนตามแนวคิดการให้เหตุผลเชิงกรณีร่วมกับแนวคิดการสะท้อนคิดเพื่อเสริมสร้างความสามารถในการใช้สื่อดิจิทัลของนักเรียนประถมศึกษา
Authors:   , สันทัดรบ พรศิริ 
Published: April 2023

การวิจัยครั้งนี้เป็นการวิจัยเพื่อพัฒนารูปแบบการเรียนการสอนสำหรับการเสริมสร้างความสามารถในการใช้สื่อดิจิทัลของนักเรียนประถมศึกษา โดยใช้แนวคิดการให้เหตุผลเชิงกรณีร่วมกับแนวคิดการสะท้อนคิด และและเพื่อประเมินประสิทธิผลของรูปแบบการเรียนการสอนที่พัฒนาขึ้น การดำเนินการวิจัย แบ่งออกเป็น 4 ระยะ ได้แก่ ระยะที่ 1 การศึกษาข้อมูลพื้นฐานเพื่อใช้ในการพัฒนารูปแบบการเรียนการสอน ระยะที่ 2 การพัฒนารูปแบบการเรียนการสอน ระยะที่ 3 การศึกษาประสิทธิผลของรูปแบบการเรียนการสอน และระยะที่ 4 การนำเสนอผลการพัฒนารูปแบบการเรียนการสอน ในการประเมินประสิทธิผลของรูปแบบการเรียนการสอน ผู้วิจัยนำรูปแบบการเรียนการสอนที่พัฒนาขึ้นไปทดลองใช้กับกลุ่มตัวอย่างที่เป็นนักเรียนประถมศึกษา สังกัดสำนักงานคณะกรรมการการอุดมศึกษา จำนวน 35 คน ใช้เวลาในการทดลอง 6 สัปดาห์ เครื่องมือที่ใช้ในการวิจัย ได้แก่ แบบวัดความสามารถในการใช้สื่อดิจิทัลของนักเรียน และแบบสังเกตพฤติกรรมความสามารถในการใช้สื่อดิจิทัลของนักเรียน การวิเคราะห์ข้อมูลโดยการเปรียบเทียบคะแนนความสามารถในการใช้สื่อดิจิทัลก่อน และหลังการทดลอง โดยใช้สถิติทดสอบค่าทีและการวิเคราะห์ความแปรปรวนทางเดียวแบบวัดซ้ำ รวมทั้งการวิเคราะห์เนื้อหา ผลการวิจัยสรุปได้ดังนี้ 1. รูปแบบการเรียนการสอนที่พัฒนาขึ้นมีวัตถุประสงค์เพื่อเสริมสร้างความสามารถในการใช้สื่อดิจิทัลของนักเรียนประถมศึกษา โดยมีหลักการ 4 ประการ คือ 1) การส่งเสริมให้ผู้เรียนเปรียบเทียบ ตรวจสอบ และวิพากษ์วิจารณ์ปัญหาหรือสถานการณ์อย่างหลากหลาย จะช่วยให้ผู้เรียนได้ประเมินปัญหาหรือสถานการณ์ และเลือกข้อมูลพร้อมให้เหตุผลประกอบ รวมทั้งทบทวนความคิด ความรู้สึกของตนเอง 2) การรวบรวมข้อมูลและสำรวจข้อมูลจากหลายแหล่ง ช่วยให้เกิดการเปรียบเทียบความน่าเชื่อถืออย่างมีเหตุผลก่อนนำข้อค้นพบไปปรับใช้ 3) การให้ผู้เรียนได้ทบทวนต่อเหตุการณ์หรือประสบการณ์ที่เกิดขึ้น เชื่อมโยงกับความรู้และประสบการณ์ของตนเองกับผู้อื่น ช่วยให้ผู้เรียน เข้าใจเหตุการณ์ลึกซึ้งขึ้น ได้ข้อสรุปหรือมุมมองใหม่ เพื่อนำมาปรับปรุง แก้ไขพร้อมให้เหตุผลประกอบ และ 4) การพิจารณาความรู้และประสบการณ์ โดยทำความเข้าใจและอธิบายสิ่งที่เกิดขึ้น ช่วยให้ผู้เรียนเกิดการเรียนรู้จากประสบการณ์ และนำมาสรุปเป็นความคิดรวบยอด และจัดเก็บเป็นคลังความรู้ เพื่อเสนอเป็นทางเลือกในการจัดการกับปัญหาหรือสถานการณ์ที่คล้ายคลึงกันในอนาคตต่อไป โดยขั้นตอนของรูปแบบการเรียนการที่พัฒนาขึ้นประกอบด้วย 3 ขั้นตอน คือ 1) เรียนรู้กรณีเดิม 2) เชื่อมโยงสู่กรณีใหม่ และ 3) ลงข้อสรุปและขยายทางความคิด 2. ประสิทธิผลของรูปแบบการเรียนการสอน พบว่า คะแนนเฉลี่ยความสามารถในการใช้สื่อดิจิทัลในภาพรวมสูงกว่าก่อนการทดลองอย่างมีนัยสำคัญทางสถิติที่ระดับ .05 3. นักเรียนที่ได้รับการจัดการเรียนการสอนโดยใช้รูปแบบการเรียนการสอนเสริมสร้างความสามารถในการใช้สื่อดิจิทัลด้วยแนวคิดการให้เหตุผลเชิงกรณีร่วมกับแนวคิดการสะท้อนคิดมีพฤติกรรมการใช้สื่อดิจิทัลเปลี่ยนแปลงไปจากเดิมโดยมีพฤติกรรมการใช้สื่อดิจิทัลที่เหมาะสมมากขึ้น
Effect of chlorhexidine gluconate on dentin carious lesion in vitro
Authors:   , Borompiyasawat Patcharanun 
Published: April 2023

Objective:The purpose of this study were to compare mean mineral density(MMD) and examine the remineralized pattern of carious dentin after cavity disinfectant with chlorhexidine gluconate (CHX) and restore with H-GIC in vitro. Materials and Methods: Selective caries removal to leathery dentin was performed in forty extracted primary molars. The samples were scanned using micro-computed tomography (micro-CT) as the MMD baseline and randomly divided into 4 groups: Group A (n=10) applied dentin conditioner and restored with H-GIC (Equia Forte™), Group B (n=10) disinfected the cavity with 2% CHX for 1 minute before applied dentin conditioner and restored with H-GIC (Equia Forte™), Group C (n=10) restored with H-GIC (Ketac Universal™) and Group D (n=10) disinfected the cavity with 2% CHX for 1 minute before restored with H-GIC (Ketac Universal™). After restoration, all samples were scanned micro-CT as the MMD after restoration. All samples were subjected to pH cycling process for 14 days and scanned micro-CT as the MMD after pH cycling. One sample from each group was randomly selected to analyze by the scanned electron microscope (SEM). Results: The comparison of MMD gain after restoration among 4 groups was a significant difference between EquiaTM and CHX-KetacTM group (oneway ANOVA with Post hoc (Tukey) test, P = 0.045). Group A: the MMD gain after restoration (±SD) was 88.81 (±59.857), group B; 168.29 (±100.899), group C; 165.54 (±72.366) and group D; 183.00 (±73.096) mgHA/ccm. Moreover, there was a significant difference of the MMD gain after restoration between EquiaTM and CHX-EquiaTM group (Independent t-test, P = 0.046). But between KetacTM and CHX-KetacTM group, there was no difference. From SEM, CHX-KetacTM group had the smallest dentinal tubule orifices and the thickest intertubular dentin among 4 groups. CHX-EquiaTM group had thicker intertubular dentin than EquiaTM group. Conclusion: The groups with 2% CHX as a cavity disinfectant had higher MMD gain and thicker intertubular dentin than non-CHX group. Therefore, the application of 2% CHX on demineralization dentin enhances the remineralization of contacted dentin underneath the restoration.
Antioxidant and antimicrobial activities of crude extracts of actinomycetes from soils
Authors:   , Wai Khaing 
Published: April 2023

A total of 17 actinomycetes isolated from soil collected from Nong Khai, Nakhon Ratchasima, Khon Kaen, Phichit, Chachoengsao and Udonthani Province, were evaluated on antimicrobial and antioxidant activities. They were identified as Streptomyces (16 strains) and Micromonospora (1 strain) based on their phenotypic and chemotaxonomic characteristics including 16S rRNA gene sequences analysis. Strain JA03 showed 98.95% similarity of 16S rRNA gene sequence to S. puniciscabiei DSM 41929T. Strain JA03 was found to be the novel species of genus Streptomyces based on whole genome average nucleotide identity (ANI). All these strains were evaluated on antimicrobial and antioxidant activities. Based on agar disk diffusion method, strain NE2-6 closely related to Streptomyces alboniger NRRL B 1832T, has the most potent antimicrobial activity against K.rhizophila ATCC 9341 and mild activity against S.aureus ATCC 25923. ET1-12 was effective on C. albicans ATCC 10231 and on K. rhizophila ATCC 934 and on B.subtilis ATCC 6633. ET2-2 closely related to Streptomyces diastaticus subsp. ardesiacus NRRL B 1773T exhibited moderate activity against B.subtilis ATCC 6633 and K. rhizophila ATCC 9341. CT2-4 closely related to Streptomyces capoamus JCM 4734T showed moderate effect on S.aureus ATCC 25923. Strain JA03 closely related to Streptomyces puniciscabiei DSM 41929T showed the highest phenolic content (33.4 µg GAE/ mg of extract) based on TPC assay and MKP33 closely related to Micromonospora yasonensis DS 3186T showed the highest FRAP value (135 µg AAE/ mg of extract) based on FRAP assay. Strain CT1-17 closely related to Streptomyces olivaceus NRRB L 3009T, showed potent scavenging activity (80.21±0.04%) at 1,000 µg/ml with IC50 value of 218.6±27.4 µg/ml based on DPPH assay. Strain MKP33 showed more potent in nitric oxide scavenging (49.3±1.96%) at 100 µg/ml than NE2-6 (49.6±1.34%) at 500 µg/ml. Cytotoxic effects of strains NE1-12, ET3-23, CT1-17, NE2-6, CT2-4, CT2-10, JA03 and MKP33 on RAW264.7 macrophage cells were evaluated by % cell viability. Strains NE1-12, CT1-17, JA03 and MKP33 showed no cytotoxic effect at 200µg/mL and strains CT2-4, CT2-10, ET3-23 and NE2-6 at 10 µg/ml on RAW 264.7 macrophage cells. Strain ET3-23 inhibited intracellular ROS generation (33±6.56%) in H2O2 (100µM) induced RAW 264.7 cells. CT2-10 at 10 µg/mL significantly inhibited NO production induced by LPS (100ng/ml) in RAW264.7 macrophage cells when compared with MKP33 at 200 µg/ml (13.87± 1.78µM and 2.9±1.02µM, respectively). In conclusion, these findings confirmed that Strain JA03 is the novel species of genus Streptomyces and possesses antioxidant activity. The actinomycetes isolated from soil provided the activity information in the development of good candidates for antibiotics and antioxidants.
Development of hyaluronic acid-based nanogel platform for improved stability and polyvalent ligand display of poly(I:C) as an effective immunostimulatory adjuvant in cancer immunotherapy
Authors:   , Kotcharat Nararat 
Published: April 2023

The aim of this research was to develop a nanogel formulation-based on modified natural polymer, hyaluronic acid (HA), as a biodegradable material for adjuvant delivery. Polyinosinic:polycytidylic acid (poly(I:C)) have been approved by FDA as a promising adjuvant candidate for the TLR3 (Toll-Like Receptor 3) activation. However, it suffers from being poor stability and is subjected to rapid enzymatic hydrolysis in serum, so that it requires high administered dose leading to adverse effects. To augment the adjuvant stability and protection from the degradation, the nano-particulate carriers were herein designed with self-assembly of HA scaffold grafted with poly (N-isopropylacrylamide), or pNIPAM. The grafting was processed through amide formation using the coupling agent (EDC/NHS). 1H NMR was carried out to confirm the modified products (HA-g-pNI). The physical incorporation of the nucleic acid into the grafted HA nanogel was achieved by incubation method with the poly(I:C) concentrations of 0.2, 1, and 10 (μg/mL) in formulations by using 0.1, 0.25, and 0.5 (% w/v) of HA-g-pNI to form the nanogel particles. The mean size, size distribution and surface charge of the nanogel particles were determined by dynamic light scattering (DLS). The particle morphology was investigated by transmission electron microscopy (TEM). Results demonstrated that HA-g-pNI with a 4 % degree of substitution were formed into nearly spherical nanogel particles with the size of approximately submicron range. The particles presented a negative value in zeta-potential. Moreover, we founded that the average size and polydispersity index (PDI) of the loaded particles were decreased significantly upon continuous incubation showing that poly(I:C) was entrapped. The stability against fetal bovine serum (FBS) degradation of the poly(I:C)-loaded nanogels was demonstrated by gel electrophoresis. Furthermore, cell viability of all HA-g-pNI formulations showed more than 80% of cell viability via RAW 264.7 macrophages. The poy(I:C)-loaded nanogel particles were excellent internalized by RAW 264.7 macrophages after 24 hrs of incubation. Therefore, the development of these poly(I:C)-loaded grafted HA nanogels will lead to a new generation of smart materials that can be functionalized and optimized for different medical purposes.
Development of recombinant human epidermal growth factor production in Nicotiana benthamiana
Authors:   , Hanittinan Oranicha 
Published: April 2023

Human epidermal growth factor (hEGF) is a short polypeptide that has gained clinical importance in the recent decade for wound healing. Currently, plant-based expression system is considered as an alternative viable platform for low-cost recombinant protein production. Hence, this study aims to produce hEGF in Nicotiana benthamiana plants via., transient expression using a geminiviral vector. The hEGF gene constructs were designed into six different constructs. The optimal expression was observed from the construct targeting the endoplasmic reticulum with C-terminal histidine tag at the yield level up to 15.695 µg/g LFW or 0.499% TSP. The plant-produced hEGF was purified by using Nickel affinity chromatography and confirmed its identity by SDS-PAGE and Western blot probed with anti-hEGF antibody. Furthermore, the preliminary study of the protein purification efficiency was found that the high extraction volume allows better hEGF recovery and extraction buffer pH 4 could largely remove host cell proteins (HCP), especially RuBisCO. However, ammonium sulfate precipitation is inapplicable for removing HCP from plant-produced hEGF. Furthermore, the study showed that no cytotoxic effects have been found in HaCaT cells from the plant-produced hEGF which is equivalent to commercial hEGF in HaCaT cells. Hence, this study supports that the potential of plant expression system offers a simple and cost-effective approach for the industrial-scale production of recombinant hEGF.
Production of recombinant protein ltb-ctla4 by transient plant-based expression using geminiviral vector
Authors:   , Yiemchavee Sutita 
Published: April 2023

Cancer is one of the leading global causes of death. In 2018, WHO estimated that 9.6 million patients died from cancer, with 18.1 million new cases emerged. In recent years, immunotherapy is considered as a treatment improving cancer-related immune function by manipulating the immune checkpoints in the immune system. Cytotoxic-T-lymphocyte antigen 4 (CTLA-4) is a immune checkpoint transmembrane protein. The expression of CTLA-4 on the surface of activated T-cell could downregulate the production and proliferation of cytotoxic T-lymphocyte cells (CTLs). CTLs are the host-defended immune cells that could retard the growth of cancer cells by recognizing the tumor-associated antigens expressed on the membrane of cancer cells, then eliminate them before spreading and evading throughout the body. The overexpression of CTLA-4 founded in cancer has a suppressive effect on cytotoxic T-lymphocyte cells, encouraging the tumor cell's growth. Recently, the monoclonal antibodies targeting CTLA-4 have successfully proved to be an active agent in immunotherapy. However, the large amount and multiple dosing had been a major hindrance. In this study, we developed heat-labile enterotoxin B subunit (LTB) fused CTLA-4 or LTB-CTLA4 as a cancer vaccine, to reduce the disadvantage in term of quantity, price, and frequency of dosing. LTB-CTLA4 was produced in Nicotiana benthamiana plant as a bio-factory, a promising platform for the plant-derived biopharmaceutical production which has abilities to produce high value but cost-effective vaccines. The highest LTB-CTLA4 level reached 1.29 µg per gram plant fresh weight and induced BALB/c mice to produce specific IgG against CTLA-4. LTB-CTLA4 has a potential as a candidate agent for cancer vaccine either to prevent the development of cancer or to treat cancer in the future.
Pharmacokinetic and metabolomic studies of standardized extract of centella asiatica (ECa 233) capsule in Thai healthy volunteers
Authors:   , Songvut Phanit 
Published: April 2023

A well-characterized standardized extract of Centella asiatica (ECa 233) being developed as a phytopharmaceutical product for human use was explored in this clinical study. The first objective of this research was to understand changes in disposition kinetics after oral administration of both the original-formula capsule and a newly-modified enhanced-dissolution capsule of ECa 233. Secondly, this study aimed to investigate alteration of human metabolomes following administration of the modified capsule of ECa 233, using an NMR-based metabolomics approach. For pharmacokinetics, the study involved a phase I clinical trial in twelve healthy Thai volunteers, who each received 250 and 500 mg of the original ECa 233 capsule and of the modified ECa 233 capsule, first as a single dose and then a week later as once-daily doses for 7 consecutive days. The results demonstrated that two major parent compounds, madecassoside (MDS) and asiaticoside (ASS), were rarely absorbed but rather underwent extensive biotransformation with minimal renal excretion. Those unabsorbed parent compounds were substituted with two mainly-active metabolites, madecassic acid (MDA) and asiatic acid (ASA). These metabolites were likely excreted through hepatobiliary system by feces elimination. Interestingly, increasing the dose of ECa 233 resulted in significantly greater plasma levels of these active metabolites, with accumulation of asiatic acid after multiple oral administration. Considering metabolomics, this accumulation behavior could affect human metabolome, partly through an alteration between pre- and post-dose of endogenous metabolites detected in plasma. The changes in five relevant metabolites were thoroughly considered to identify candidate biomarkers; these were L-homoserine, citrulline, O-succinyl L-homoserine, homocarnosine, and choline. In particular, ECa 233 was associated with a significant increase in levels of choline, an endogenous metabolite reported to have benefits for learning and memory. This finding suggests that ECa 233 may adjust human metabolic profiles and in this way play a role in fulfilling endogenous metabolites, which might be useful in mitigating cognitive impairment in phase II clinical study.
Development of a device to alert for breaks to prevent musculoskeletal disorders in the neck and low back among office workers
Authors:   , Waongenngarm Pooriput 
Published: April 2023

The objective of this thesis was to develop a device to alert for breaks to prevent musculoskeletal disorders in the neck and low back among office workers. This thesis was divided into four stages: 1) systematically review to gain insights into the effectiveness of breaks on low back pain, discomfort, and work productivity in office workers; 2) evaluation of the characteristics of perceived discomfort and postural shifts during a 4-hour sitting period; 3) evaluation of the effects of a device to alert for active breaks on preventing neck and low back pain among office workers: 6- and 12-month follow-up; and 4) evaluation of the incidences of neck and low back pain and working from home related risk factors during the COVID-19 outbreak among office workers. The results from systematic review revealed that breaks are recommended for reducing low back pain and discomfort with no disturbance in work productivity among office workers. The type of rest breaks that may be effective in reducing low back pain and discomfort was identified, namely active breaks with postural change. In the second study, our findings suggest that prolonged sitting for longer than 30 minutes possibly increase the risk of neck and low back pain, which used to develop the device to alert for breaks. The device to alert for break was developed by the author and engineering team, which consists of three components: seat pad, controller and smartphone application. This device can detect sitting time and recommend break duration during work to the user. The device had good to excellent validity and consistency. The results of the effectiveness of the device on preventing neck and low back pain showed that office workers who received the device to alert for breaks significantly reduced the 6- and 12-month incidence rate of neck and low back pain. In addition, the incidence of neck and low back pain during the COVID-19 period was lower than that for during the pre-COVID-19 period. The number of days working from home per week was associated with the incidence of neck and low back pain during COVID-19 period.
Fabrication and characterization of graphene-based heterostructure using molybdenum as alternative electrodes
Authors:   , Klanurak Illias 
Published: April 2023

Interlayer coupling in graphene heterostructures can be used to modify their charge transport properties for the use in nanoelectronics applications. To achieve high-performance devices, graphene is typically encapsulated by two thin sheets of hexagonal boron nitride (hBN) and contacted using Cr/Au as electrodes. ​ In this research, Mo was selected as alternative stand-alone electrodes to simplify the fabrication process. We have developed a recipe for the fabrication of field-effect transistor (FET) using twisted bilayer graphene (tBLG) as a transport channel. Mo was employed to make one-dimensional (1D) contacts to the edges of graphene by sputtering. Electronic transport measurement was then performed on the FET devices to investigate the quality of Mo contacts. We achieved ohmic contacts between Mo and graphene. The contact resistivity between Mo and graphene reached a maximum value of around 1,300 Ω⋅µm at charge neutrality point and decreased to 975 Ω⋅µm at a carrier density of ~4×1012 cm-2. However, we have observed that the contact resistivity increases over time likely due to the oxidization of Mo. Despite the increase in contact resistivity, the Mo contacts still exhibit ohmic behavior after 3 months and it can be used to investigate transport phenomena in graphene. In this study, the room-temperature electron and hole mobilities of tBLG reached values as high as 27,000 and 21,000 cm2/V⋅s indicating that Mo electrodes do not hinder the transport characteristics of graphene.
Comparative study of tensile bond strength between resin cements and hybrid materials
Authors:   , Donpinprai Chakriya 
Published: April 2023

Purpose: To compare tensile bond strength among the hybrid materials (VITA ENAMIC®, SHOFU Block HC, Katana AVENCIA and Experimental material) using two primers (Universal ceramic primer and RelyX ceramic primer) and two resin cements (Super-Bond C&B and RelyXTM U200). Methods: Twenty blocks of 4×4×1 mm3 were prepared from each material type. In VITA ENAMIC® group, specimens were conditioned with 5% Hydrofluoric acid and primer. In SHOFU Block HC and Katana AVENCIA groups, specimens were treated with 50µm alumina and primer. In experimental material, specimens were etched with 65% Phosphoric acid. Ten specimens were randomly assigned to each group for different resin cement then cemented on bovine enamel. All cemented specimens were stored in water at 37ºC for 24 hours. TBS was tested with a universal testing machine. The fractured interface was examined with a stereomicroscope and TBS data were statistically analyzed. Result: The experimental material showed the highest TBS values, followed by VITA ENAMIC®, Katana AVENCIA, and SHOFU Block HC, respectively. There was no significant difference between different resin cement (p>0.05). In SHOFU Block HC group, all fractured specimens showed adhesive failure. Conclusion: The types of inorganic components and surface treatment of the hybrid materials have an effect on TBS values.
Effect of ultrasonic treatment on the transverse strength of relined denture base with autopolymerizing acrylic hard reline material
Authors:   , Arunwichit Tithiporn 
Published: April 2023

This study evaluated the effect of ultrasonic treatment and surface treatments on transverse strength of denture bases which relined with auto-polymerizing acrylic resin. The study groups, ninety heat-polymerized acrylic resins (64×10×2 mm) were divided by three surface treatments: no surface treatment (N), applied MMA for 180 seconds (MM), applied MF-MA for 15 seconds (MF). They were relined with auto-polymerized acrylic resin to create 64×10×3.3 mm specimens. Then, they were divided further by ultrasonic which were no ultrasonic (X), ultrasonic in water (W), ultrasonic in 30% ethanol (E). As a result, all test groups were classified as NX, NW, NE, MMX, MMW, MME, MFX, MFW, MFE (n=10). All samples were performed three-point bending test. Two-way ANOVA presented that there was an interaction between ultrasonic and surface treatments. Then One-way ANOVA was analyzed. Ultrasonic could increase transverse strength of all relined specimens (p<0.05). Ultrasonic in ethanol increased strength superior to water (p<0.05) and presented the highest mean strength. In the same ultrasonic treatment, MMA and MF-MA increased the transverse strength of relined denture bases compared with no applying groups (N) (p<0.05). There was no difference between all MM and MF groups (p>0.05).