bluuebunny/crossref_metadata_2025_split huggingface dataset:
A trend analysis of tuberculosis mortality among rural people in China from 2006 to 2020: a joinpoint and age-period-cohort analysis
Authors: ni shuaihu , Sun Ke , Luo Xiaofeng , Qu Yan 
Published: August 2023

Abstract
Objective
To investigate the mortality trends of tuberculosis among rural people in China from 2006 to 2020, and to provide a basis for formulating scientific tuberculosis prevention and control strategies.
Methods
According to the data of Chinese Disease Surveillance Points, taking rural people aged 5 ~ 84 years in China from 2006 to 2020 as the research subjects, the Joinpoint regression model was used to explore the overall trend of tuberculosis mortality, and the age-period-cohort model was used to estimate the age effect, period effect and birth cohort effect.
Results
From 2006 to 2020, the standardized mortality rate of tuberculosis among rural people in China showed a downward trend. The age effect of tuberculosis deaths in the population was on the rise, with the age of men aged 35 ~ 84 and women aged 60 ~ 84 as risk factors; The period effect showed a downward trend, the rate of decline gradually slowed down, and the risk of death in males increased from 2016 to 2020; The birth cohort effect generally declined, and birth cohorts turned into protective factors after 1969 ~ 1973. Males had an increased risk of death in the birth cohorts 1964 ~ 1968, 1984 ~ 1988, 1994 ~ 1998, and 2004 ~ 2008. Females also had an increased risk of death in the birth cohort 1989 ~ 2003.
Conclusions
Rural males aged 35 ~ 84 years old and females aged 60 ~ 84 years old had a higher risk of tuberculosis death. The risk of TB death for rural people decreased overall over time, with the risk of TB death increasing for rural males from 2016 to 2020. In general, the risk of TB death is lower in late birth cohorts, However, it is alarming that the risk of TB death increased in some birth cohorts in recent years, both males and females.
Seismic hazard evaluation of Nepal region: a particular emphasis on 2015 Gorkha earthquake scenario
Authors: Saini Atul , Mohanty Debasis D , Mittal Himanshu 
Published: August 2023

Abstract
The tectonic collision between the Indian and Eurasian plates since 50 million years is responsible for creating the Nepal Himalayan zone as part of the whole Himalayan orogeny belt. As a result of this collision, major changes and deformation in Earth have been evolving through ongoing stress build-up and release. In this study, an overview of the stress distribution has been made by using the Frequency-magnitude relation across the Nepal Himalayan region, which is supported by an extensive analysis of b-values and their spatiotemporal variations. Abruptly the whole region is being divided into four sub-regions. The consequences of b-value changes have been thoroughly examined for a better and minute analysis of the present-day hazardous scenario of this Nepal Himalayan region. Two highly stressed areas have been observed in Nepal's eastern and westernmost parts by interpreting the b-values. The depth sections of the b-value are estimated across the two zones revealing that these stress-accumulated regions are increasing in depth with an increase in latitude towards the north. A moderately stressed area was also found, which is concentrated mainly around the MCT fault in the western part. A particular emphasis on the 2015 Gorkha region has been carried out to analyze this region's pre- and post-earthquake scenarios and stress patterns. The analysis revealed that most of the stress released by the Gorkha earthquake was concentrated in the western side of the event, whereas the eastern side was still moderately stressed.
Towards Genomic Medicine: A Tailored Next-Generation Sequencing Panel for Hydroxyurea Pharmacogenomics in Tanzania
Authors: Nkya Siana , Nzunda Collin , Saukiwa Emmanuel , Kaywanga Frida , Buchard Eliud , Solomon David , Christopher Heavenlight , Ngowi Doreen , Johansen Julieth , Urio Florence , Mgaya Josephine , Karim Salman , Alimohamed Mohamed Zahir , Sangeda Raphael Z. , Chamba Clara , Chimusa Emile R. , Novelli Enrico , Makani Julie 
Published: August 2023

Abstract

Pharmacogenomics of hydroxyurea is an important aspect in the management of sickle cell disease (SCD), especially in the era of genomic medicine. Genetic variations in loci associated with HbF induction and drug metabolism are prime targets for hydroxyurea (HU) pharmacogenomics. This study investigated genetic variations in BCL11A, ARG2, HBB, HBG1, WAC, HBG2, HAO2, MYB, SAR1A, KLF10, CYP2C9, CYP2E1 and NOS1 as potential HU pharmacogenomics targets. The panel was designed using the Illumina Design Studio (Illumina, San Diego, CA, USA) and achieved a total coverage of 96% of all genomic targets over a span of 51.6 kilobases (kb). We are reporting a successfully designed Illumina (MiSeq) HU pharmacogenomics custom panel encompassing 51.6 kilobases. The designed panel achieved greater than 1000x amplicon coverage which is which is sufficient for genomic analysis. Therefore, this study provides a valuable tool for research in HU pharmacogenomics, especially in Africa.
Morpho-Histopathological Assessment of Platelet-Rich Fibrin, Stem Cell-Derived Micro vesicles, and ZnO Nanoparticles on Achilles Tendon Healing in Rabbits
Authors: Hassan Mahmoud A. , Elgohary Islam , Abdallah Ahmed F. , Nassif Mahmoud T. , El-Sherif Mohamed W. 
Published: August 2023

Abstract
The objective of this study was to evaluate the effect of zinc oxide nanoparticles (ZnONP), mesenchymal stem cell-derived micro vesicles (MVs), and Advanced platelet-rich fibrin (A-PRF) on the healing of Achilles tendon defects in rabbits during the inflammatory, proliferative, and remodeling phases through histological examination and quantitative assessments. Twenty-four adult male New Zealand albino rabbits with Achilles tendon defects were divided into four equal groups of six animals each. In the control group, the Achilles tendon defect was left untreated. In the ZnONP group, the defect was injected with 0.5 ml of 0.2% ZnONP. In the MVs group, MSC-derived MVs were injected into the defect twice within one week apart. In the A-PRF group, the defect was filled with a PRF membrane. Tendon tissue was harvested at 2, 4, and 6 weeks after treatment and sections were stained with hematoxylin-eosin and Masson trichrome. Histological examination revealed that proliferation and remodeling of collagen fibers and approximation of normal tendinous tissue occurred earlier in the ZnONP group after 6 weeks than in other treated groups. This study demonstrated that ZnONP have a significant ability to enhance and accelerate the healing and regeneration processes of the Achilles tendon.
Evaluation of Endometrial Receptivity in Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure
Authors: Canan Sultan , İnan Mehmet Arda , Erdem Ahmet , Demirdağ Erhan , Gündüz Mualla İlknur , Erdem Özlem , Erdem Mehmet 
Published: August 2023

Abstract
Background: The cause of implantation defects in patients with recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) has not been clearly established. We aimed to evaluate the immunohistochemical changes of HOXA-11, β1 integrin, Focal Adhesion Kinase (FAK), CD44, and Extracellular Matrix Protein 1 (ECM1) in the endometrium of patients with defective implantation -RIF and RPL- in the receptive period.
Methods: This retrospective case-control study was conducted in a university hospital. After the exclusion of all cases with pathology that may cause a change in the level of receptors in the endometrium, biopsies taken during the receptive period were selected, and the patients were divided into RPL (n=15), RIF (n=16), and control (n=16) groups. All preparations were immunohistochemically stained for HOXA11, β1 integrin, FAK, CD44, and ECM1. The presence or absence of staining and staining intensity were compared between groups.
Results: When the RIF group was compared with the control group, there was no significant difference in HOXA-11 and β1 Integrin expression (p>0.05), FAK expression was significantly increased compared to the control group (p<0.01); ECM1 and CD44 expressions were found to be significantly decreased (p<0.01). When the RPL group was compared with the control group, there was no significant difference in the endometrial staining of HOXA-11, FAK, and ECM1 in women with a history of RPL (p>0.05); β1 Integrin and CD44 levels were significantly decreased (p<0.05).
Conclusion: CD44 reduction may play a role in pathophysiology in both patient groups. In addition, decreased β1 integrin may also play a role in RPL. In RIF, the increase in FAK and decrease in ECM1 seem to be involved in the mechanisms of the disease.
5-Hydroxymethylcytosine profiles in circulating cell-free DNA serve as potential biomarkers for diagnosis and classification of adenomyosis
Authors: zhang Lei , Han Xiaotong , Chen Hangyu , Shang Chunliang , Chen Long , Lin Jian , Guo Hongyan 
Published: August 2023

Abstract
Background:
At present, clinical diagnosis and classification of adenomyosis are mainly dependent on MRI or pathological examination, and little research is carried out on molecular biomarkers for the diagnosis and classification of adenomyosis. Our aim is to identify molecular biomarkers for the diagnosis and classification of adenomyosis.
Methods:
5hmC-Seal technique was used to obtain genome-wide 5hmC profiles from plasma cfDNA and tissue genomic DNA samples. Patients were divided into the training (n = 26) and the validation group (n = 25), and a 5hmC-based Logistic regression model from the training group was developed to verify the diagnostic capability of the model. Meanwhile, we investigated whether 5hmC molecular markers could be used to classify the two main subtypes of adenomyosis.
Results:
In this study, ten 5hmC markers were identified by using machine learning techniques. The diagnostic ability reached 0.88 sensitivity and 0.87 specificity (AUC = 0.91). Next, we found that 5hmC markers can be used as markers for adenomyosis classification.
Conclusions:
Our results showed that 5hmC markers of cfDNA had the potential to be used for diagnosis and classification in adenomyosis patients, and 5hmC-Seal may be a clinically applicable and minimally invasive method for the diagnosis and classification of adenomyosis in the future.
Development and validation of a multi-modal ultrasomics model to predict response to neoadjuvant chemoradiotherapy in locally advanced rectal cancer
Authors: Qin Qiong , Gan Xiangyu , Lin Peng , Pang Jingshu , Gao Ruizhi , Wen Rong , Liu Dun , Tang Quanquan , Liu Changwen , He Yun , Yang Hong , Wu Yuquan 
Published: August 2023

Abstract
Objectives
To assess the performance of multi-modal ultrasomics model to predict efficacy to neoadjuvant chemoradiotherapy (nCRT) in patients with locally advanced rectal cancer (LARC) and compare with the clinical model.
Materials and methods
This study retrospectively included 106 patients with LARC who underwent total mesorectal excision after nCRT between April 2018 and April 2023 at our hospital, randomly divided into a training set of 74 and a validation set of 32 in a 7: 3 ratios. Ultrasomics features were extracted from the tumors’ region of interest of B-mode ultrasound (BUS) and contrast-enhanced ultrasound (CEUS) images based on PyRadiomics. Mann-Whitney U test, spearman, and least absolute shrinkage and selection operator algorithms were utilized to reduce features dimension. Five models were built with ultrasomics and clinical analysis using multilayer perceptron neural network classifier based on python. Including BUS, CEUS, Combined_1, Combined_2 and Clinical models. The diagnostic performance of models was assessed with the area under the curve (AUC) of the receiver operating characteristic. The DeLong testing algorithm was utilized to compare the models’ overall performance.
Results
The AUC (95% confidence interval [CI]) of the five models in the validation cohort were as follows: BUS 0.675 (95%CI: 0.481–0.868), CEUS 0.821 (95%CI: 0.660–0.983), Combined_1 0.829 (95%CI: 0.673–0.985), Combined_2 0.893 (95%CI: 0.780-1.000), and Clinical 0.690 (95%CI: 0.509–0.872). The Combined_2 model was the best in the overall prediction performance, showed significantly better compared to the Clinical model after DeLong testing (P < 0.01). Both univariate and multivariate logistic regression analyses showed that age (P < 0.01) and clinical stage (P < 0.01) could be an independent predictor of efficacy after nCRT in patients with LARC.
Conclusion
The ultrasomics model had better diagnostic performance to predict efficacy to nCRT in patients with LARC than the Clinical model.
WITHDRAWN: Review From Beneficial Arthropods to Soil-Dwelling Organisms: A Review on Millipedes in Africa
Authors: Mawcha Kahsay Tadesse 
Published: August 2023

Abstract

Millipedes are important soil-dwelling organisms that play a vital role in the soil's nutrient cycling and overall health. They can increase the availability of nitrogen and phosphorus and accelerate the decomposition of organic matter in the soil. However, they can also cause significant damage to crop plants, which can lead to reduced yields and negatively impact both soil quality and plant health. Integrated Pest Management (IPM) strategies may be necessary to effectively manage millipedes. These strategies involve a combination of cultural and chemical control methods that are tailored to specific crops and environmental conditions. By taking a holistic, ecosystem-based approach, IPM strategies can effectively manage millipede populations while minimizing negative impacts on the environment. Monitoring millipede activity and implementing control measures in areas with a high infestation is a crucial measure. This review provides recent research progress on millipede’s biology, ecology, and agriculture pest status of millipedes in Africa and beyond and IPM strategies to control their infestation in agricultural crops.
Functional analysis of the wheat class III peroxidase gene TaPer12-3A in seed dormancy and germination
Authors: Gao Wei , Jiang Yating , Yang Xiaohu , Li Ting , Zhang Litian , Yan Shengnan , Cao Jiajia , Lu Jie , Ma Chuanxi , Chang Cheng , Zhang Haiping 
Published: August 2023

Abstract

Class III peroxidases (PODs) perform crucial functions in various developmental processes and in response to biotic and abiotic stresses. However, the specific roles of class III POD genes in wheat seed dormancy (SD) and germination remain elusive. Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively, indicating a significant association with SD and germination. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests indicated that TaPer12-3A negatively regulated SD and positively mediated germination in transgenic Arabidopsis and rice lines as well as in a wheat mutant mutagenized with ethyl methane sulfonate (EMS) in Jing 411 background. Further studies demonstrated that TaPer12-3A regulated SD and germination by interacting with gibberellin and abscisic acid biosynthesis, catabolism, and signaling pathways in transgenic rice seeds. These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also contribute to understanding the complex regulatory mechanism involved in these processes.
Influence of digitalisation on business success in Austrian Traded Prime Market Companies – a longitudinal study
Authors: Hangl Christa 
Published: August 2023

Abstract
The paper examines whether software investments have an impact on the economic success of the companies listed on the Austrian Traded (ATX) Prime market.
A literature review and qualitative content analysis are performed to answer the research questions. For testing hypotheses, a longitudinal study is conducted. Over a ten-year period, the consolidated financial statements of the businesses under review are evaluated. A panel will assist with the data analysis. This study offers notable distinctions from other research that has investigated the correlation between digitalisation and economic success. The present research employs a methodology that involves quantifying the extent of digitalisation by conducting a thorough investigation of the group annual reports of the companies in the study.
The regression analysis of all businesses revealed no correlation between software expenditures and economic success. The qualitative content analysis demonstrated that organisations belonging to the finance sector are particularly engaged in numerous digitalisation efforts. Consequently, separate regression models were computed for finance and non-finance companies. The correlation between software investments and economic success in both industries is evident.
JEL codes: O33, E22, G21
Navigating between promises and realities of transdisciplinarity for environmental conservation
Authors: Rosa Gabriela De La , Muñiz Esther Milberg , El-Hani Charbel N. , Ludwig David 
Published: August 2023

Abstract
Conservation approaches focused solely on technical aspects have proven insufficient in addressing the complexity of the current socioenvironmental crisis. Transdisciplinarity has emerged as a promising approach to combining technical perspectives with qualitative insights from the social sciences and non-academic stakeholders. However, transdisciplinary incorporation of diverse interests, knowledge, and worldviews requires acknowledging and addressing inherent inequities. This article presents a case study that applies transdisciplinary principles through two layers of reflection. The first layer involves a biologist/ecologist working in a transdisciplinary group, applying a quick participatory diagnosis, while the second layer comprises a social scientist studying the group itself. By analyzing the results obtained from these reflective layers, the article aims to explore the challenges and possibilities of navigating from transdisciplinary ideals to practical implementation. The findings highlight the importance of nurturing relationships built on care, reciprocity, and openness to address the power imbalances inherent in university-community participatory practices. Through this perspective, the article suggests that the community's autonomy and agency can be preserved while actively engaging its members in the transdisciplinary process. These suggestions contribute to alleviating power differences and fostering a collaborative and equitable transdisciplinary framework. Overall, this study emphasizes the significance of integrating participatory methods, critical reflexivity, and attention to power dynamics in realizing the potential of transdisciplinary approaches for addressing complex conservation challenges and fostering meaningful community engagement.
Trustworthy Healthcare Cloud Storage Auditing Scheme (TCSHAS) with Blockchain-based Incentive Mechanism
Authors: Bose Rajesh , Sutradhar Sharabani , Bhattacharyya Debnath , Roy Sandip 
Published: August 2023

Abstract
In this paper, we present the Trustworthy Healthcare Cloud Storage Auditing Scheme (TCSHAS) to address the trust issues associated with third-party auditors (TPAs) in traditional healthcare cloud storage audit schemes. Leveraging the non-tamperability and traceability features of blockchain technology, we propose a novel incentive mechanism that promotes honest and reliable behaviors among TPAs. Our approach involves organizing TPAs as a group of blockchain nodes that engage in mutual surveillance, ensuring the maintenance of a reputable group by inspecting and punishing malicious behaviors severely. We provide a comprehensive system model for the TCSHAS, which incorporates smart contracts to handle transactions-related matters, including dispute resolution. Through performance evaluations, we demonstrate that our scheme offers acceptable efficiency and is well-suited for real-world healthcare applications. By utilizing blockchain technology and a robust incentive mechanism, the TCSHAS enhances trust and security in healthcare cloud storage auditing, providing a foundation for reliable and accountable management of sensitive healthcare data.
Exploring the Mechanism and discovery of potential hits to improve the treatment of pyrazinamide-resistant Mycobacterium tuberculosis
Authors: Ullah Numan , Shahab Muhammad , Yaseen Muhammad , Bhat Mashooq Ahmad , Gul Mian , Zheng Guojun , Akter Shahina 
Published: August 2023

Abstract
The rise of antibiotic-resistant Mycobacterium tuberculosis (MTB) has led to a reduction in the availability of effective medications for tuberculosis therapy, resulting in increased morbidity and mortality globally. Tuberculosis primarily affects the lungs but can also spread to the brain and spine. Pyrazinamide (PZA) is considered to be the most potent drug but it also shows resistance due to mutation at the position G97D. However, the Mechanism of PZA drug resistance towards Mtb PZase is unclear. Moreover, due to mutations that provide PZA resistance, there is no such evidence about the structural dynamics of Mtb PZase protein. Here we aimed to perform molecular docking, pharmacophore modeling, virtual Screening, and MD simulation, to explore the Mtb PZase protein resistance mechanism toward the PZA drug due to mutation at the G97D position as well as compare the Wild-type and Mutant-type PZA bound systems. From the analysis, the Wild-type shows good interaction as compared to Mutant-type PZA bound systems. Then we performed pharmacophore-based virtual screening against commercially available databases i.e. ZINC and Chembridge. Finally, we selected a total of 5 lead compounds based on docking score and good interaction. The selected compounds considered has a good ADMET property, because the compound shows no pan Assay interference (PAINS) indicating that these compound could be useful inhibitors of Mutant-type PZA bound systems.
A system dynamics-based model for the evolution of environmental group events
Authors: Qi Xue-ting , Bu Fanliang 
Published: August 2023

Abstract

Based on the system dynamics theory, this paper establishes an environmental mass event evolution model and explores the evolution law of mass events caused by environmental problems. From a methodological point of view, the mixed-strategy evolutionary game principle and dynamic punishment measures are combined, and simulation analysis is carried out by Anylogic software, and the results show that there is no stable evolutionary equilibrium solution for the two sides of the game in the traditional asymmetric mixed-strategy game model, and after adjusting the game payoff matrix and incorporating the dynamic punishment strategy, stable evolutionary equilibrium solutions appear in the evolutionary game model, and the system begins to tend to be stabilized. The process and conclusions of the simulation experiment provide methodological reference and theoretical support for the analysis of the evolution of environmental mass events.