bluuebunny/crossref_metadata_2025_split huggingface dataset:
DHX36 maintains genomic integrity by unwinding G‐quadruplexes
Authors: Mizumoto Ayaka , Yokoyama Yuta , Miyoshi Tomoichiro , Takikawa Masahiro , Ishikawa Fuyuki , Sadaie Mahito 
Published: August 2023

AbstractThe guanine‐rich stretch of single‐stranded DNA (ssDNA) forms a G‐quadruplex (G4) in a fraction of genic and intergenic chromosomal regions. The probability of G4 formation increases during events causing ssDNA generation, such as transcription and replication. In turn, G4 abrogates these events, leading to DNA damage. DHX36 unwinds G4‐DNA in vitro and in human cells. However, its spatial correlation with G4‐DNA in vivo and its role in genome maintenance remain unclear. Here, we demonstrate a connection between DHX36 and G4‐DNA and its implications for genomic integrity. The nuclear localization of DHX36 overlapped with that of G4‐DNA, RNA polymerase II, and a splicing‐related factor. Depletion of DHX36 resulted in accumulated DNA damage, slower cell growth, and enhanced cell growth inhibition upon treatment with a G4‐stabilizing compound; DHX36 expression reversed these defects. In contrast, the reversal upon expression of DHX36 mutants that could not bind G4 was imperfect. Thus, DHX36 may suppress DNA damage by promoting the clearance of G4‐DNA for cell growth and survival. Our findings deepen the understanding of G4 resolution in the maintenance of genomic integrity.
Angela Rayner (Member of Parliament) and the “Basic Instinct Ploy”: Intersectional misrecognition of women leaders' legitimacy, productive resistance and flexing (patriarchal) discourse
Authors: Stead Valerie , Mavin Sharon , Elliott Carole 
Published: August 2023

AbstractThis paper interrogates a shift in patriarchal media discourse related to women leaders' recognition and legitimation in the UK. We conduct a multimodal discourse analysis of an online newspaper article about the UK politician and Deputy Leader of the Labour Party, Angela Rayner, and analyzed public responses. Understanding the media as a means to distribute power and enable the challenging of norms, we contribute a theory of intersectional misrecognition in media's representation of women political leaders. This reveals an enduring and dynamic subordinate status of women leaders, shown specifically through the intersection of gender and class. We theorize that while women leaders continue to be misrecognized in the media, destabilizing their legitimacy, there is a demonstrable flexing of patriarchal discourse combined with stronger and accelerated resistance to ongoing sexism. We identify this resistance as productive in its call for consequences and a redistribution of cultural values, reflecting a discursive shift toward a productive resistance of resilient gender norms, evident in the intersection of gender with class. Intersectional misrecognition has value in making inequalities explicit for women leaders and where there may be productive tensions with potential to mobilize for change.
Impact of albumin–lymphocyte–platelet–C‐reactive protein index as a prognostic indicator of hepatocellular carcinoma after resection: Associated with nuclear factor erythroid 2–related factor 2
Authors: Iseda Norifumi , Itoh Shinji , Toshida Katsuya , Nakayama Yuuki , Ishikawa Takuma , Tsutsui Yuriko , Izumi Takuma , Bekki Yuki , Yoshiya Shohei , Toshima Takeo , Yoshizumi Tomoharu 
Published: August 2023

AbstractAimTo investigate the prognostic value of the preoperative albumin–lymphocyte–platelet–C‐reactive protein (ALPC) index in patients with hepatocellular carcinoma (HCC) undergoing curative hepatectomy. We also evaluated the relationship between the ALPC index and the phosphorylated nuclear factor erythroid 2‐related factor 2 (p‐Nrf2) levels.MethodsData were analyzed retrospectively from 256 patients who underwent resection for HCC. For cross‐validation, patients were divided into the training and testing cohort. We assessed eight combinations of inflammatory markers for predictive value for recurrence. We examined the associations of the ALPC index with recurrence‐free survival and overall survival in univariate and multivariate analyses (Cox proportional hazards model). Immunohistochemical staining of p‐Nrf2 was performed on tumor samples of 317 patients who underwent hepatic resection for HCC.ResultsA high preoperative ALPC index correlated with a high serum albumin concentration, small tumor size, low rate of poor differentiation, solitary tumor, early Barcelona Clinic Liver Cancer stage, and low rate of microscopic intrahepatic metastasis in the training dataset. A high preoperative ALPC index correlated with a high serum albumin concentration, high serum alpha‐fetoprotein concentration, small tumor size, a low rate of poor differentiation and a low rate of microscopic intrahepatic metastasis in the testing dataset. A higher preoperative ALPC index was an independent predictor of longer recurrence‐free survival and overall survival in the training and testing datasets. A high ALPC index was associated with negative p‐Nrf2 expression in HCC tumor cells.ConclusionsWe showed that a high ALPC index was an independent prognostic factor for patients with HCC undergoing curative hepatic resection.
Influence of sunscreen formulation on the transfer of mineral and organic ultraviolet filters from skin to seawater in simulated ocean bathing tests
Authors: Saxe Jennifer K. , Mullins Larry , Jones Randy , Lewis Alan , Sun Frank , Reynertson Kurt A. 
Published: August 2023

AbstractObjectiveSignificant research and regulatory attention have been focussed on the potential for some ultraviolet filters (UVFs) to rinse off from beachgoers' skin into seawater leading to exposure to sea life, especially coral reefs. The amount of UVFs potentially rinsed from skin during recreational beach activities has not been well studied, leading to uncertainty about the potential magnitude of aquatic UVF exposure due to changes in sunscreen use patterns. This study quantifies rinse‐off of UVFs in sunscreen from skin into synthetic seawater and identifies differences in rinse‐off quantity due to formulation type with a goal of informing future modelling efforts aimed at estimating UVF exposure to sea life associated with recreational activities at the beach.MethodsUVF rinse‐off from skin during recreation in seawater was simulated by applying eight different sunscreen products to porcine skin samples followed by three periods of shaking in synthetic seawater totalling 40 min. The rinsed mass of six UVFs – zinc oxide, titanium dioxide, avobenzone, homosalate, octisalate, and octocrylene – was determined analytically in synthetic seawater and in extractant rinsate from glassware for organic UVFs and compared among formulas.ResultsAmong the 22 UVF‐formulation combinations tested, 19 resulted in less than 10% of the applied UVF rinsed from skin. All formulation‐UVF combinations where the formula types were water‐in‐oil (reverse phase) emulsions or anhydrous resulted in 5% or less of the applied UVF rinsed to synthetic seawater. Sunscreens formulated as oil‐in‐water emulsions yielded higher rinse‐off percentages for all UVFs tested, with a maximum of 20% rinse‐off of avobenzone in one lotion.ConclusionThe potential for sunscreen UVF rinse‐off is significantly influenced by formulation and is generally well below the prior assumed rinse‐off levels used to estimate risk. Formulation consideration is therefore essential for accurate exposure models used in environmental risk assessment. Anhydrous and reverse phase (water‐in‐oil) sunscreen formulations tested resulted in lower UVF transfer from skin to synthetic seawater in simulated ocean bathing tests and as a result, are expected to yield lower UVF exposures to sea life. This approach can be used in predictive environmental exposure models to support ecologically safe sunscreen formulation design.
Exploring ultrasound‐assisted technique for enhancing techno‐functional properties of plant proteins: a comprehensive review
Authors: Justino Heloisa de Fátima Mendes , dos Santos Israel Felipe , de Souza Ruan Carlos Nascimento , Sanches Edgar Aparecido , Bezerra Jaqueline de Araújo , Lamarão Carlos Victor , Pires Ana Clarissa dos Santos , Campelo Pedro Henrique 
Published: August 2023

SummaryThe adoption of vegetarian diets has been increasingly embraced by individuals seeking to improve their health and reduce their environmental impact. One concern regarding this dietary choice is the adequate intake of proteins with nutritional quality comparable to animal‐derived proteins. In this regard, plant‐based proteins have been the focus of studies and technological developments aimed at enhancing their nutritional and functional properties. The application of ultrasound to plant proteins has proven to be an effective strategy for modifying their structure and improving their digestibility, solubility, and viscosity. This review article presents studies that have investigated the modification of plant‐based proteins using ultrasound, with emphasis on their nutritional and technological implications. The effects of ultrasound on protein structure and properties will be discussed, as well as the potential applications in the food industry.
Natural cane sugar derivative‐based pumpkin bars: biofunctional, organoleptic quality and shelf life studies
Authors: Kaur Gagandeep , Kaur Sukhpreet , Aggarwal Poonam , Kaur Navjot 
Published: August 2023

SummaryJaggery, a natural unrefined sweetener rich in phytonutrients, could be used as a healthier substitute to develop functional foods. The present study intended to utilise liquid jaggery in place of refined sugars to develop a functional fruit snack bar. Five different formulations were prepared by varying levels (0%–100%) of liquid jaggery in conventional fruit bar containing pumpkin pulp as base material. The addition of jaggery significantly enhanced bioactive constituents and minerals, which were also confirmed by ICAP analysis. Based on sensory characteristics, pumpkin bars prepared by replacing sugar with 50% liquid jaggery were selected for shelf study evaluation. Bars were packed in laminated foil and butter paper and stored under room (25 ± 1 °C) and refrigerated (4 ± 1 °C) conditions. Irrespective of storage temperature and packaging materials, significant variation was noticed in moisture content and textural characteristics of stored fruit bars. Total and reducing sugars and titratable acidity increased while bioactive constituents and colour values remarkably decreased with progression of storage period. Except moisture, the rate of loss of quality attributes was rapid at room temperature. Stored jaggery‐based pumpkin bars packed in both packagings showed satisfactory microbial and organoleptic quality for 6 months.
Development of functional pasta enriched with green leafy vegetables: impact on liposoluble compounds, nutritional, technological and sensorial quality
Authors: Fratianni Alessandra , Vitone Caroline , D'Agostino Annacristina , Trivisonno Maria Carmela , Falasca Luisa , Panfili Gianfranco 
Published: August 2023

SummaryDurum wheat semolina was replaced with 15% of freeze‐dried leaves of spinach, chicory, or chard to obtain pasta samples enriched with vegetables. Samples were evaluated for chemical composition, tocol and carotenoid content, texture, cooking, and sensorial quality. All developed pasta samples can be claimed as a “source or high source of fibres” (from 4.5 to 6/100 g) and as a “source of vitamin E and vitamin A”. A slight increase in cooking loss and a decrease of optimal cooking time was found as to semolina control sample. Spinach and chard pastas reported similar values to semolina pasta for water absorption (120%–130%), swelling index (about 1.7), and firmness (680 and 610 g, respectively) and an excellent sensorial quality. Even with a lower technological quality, chicory pasta resulted in good acceptability. Partial replacement of wheat semolina with vegetables showed the possibility of producing pasta with an improved nutritional value and good technological features.
The use of hydrotherapy in the first stage of labour: A systematic review and meta‐analysis
Authors: Ergin Ayla , Aşci Özlem , Bal Meltem Demirgöz , Öztürk Gizem Güneş , Karaçam Zekiye 
Published: August 2023

AbstractAimTo explore the effect of hydrotherapy applied in the first stage of labour on the health of mother and newborn.MethodsThis systematic review and meta‐analysis was carried out by following PRISMA. The studies were obtained by scanning EBSCO, PubMed, Science Direct, Ovid, Web of Science and Scopus electronic databases. Twenty studies published between 2013 and 2023 were included.ResultsThe total sample size of the studies was 8254 (hydrotherapy: 2953, control: 5301). Meta‐analyses showed that the perception of pain decreased, comfort level and vaginal birth rate were higher and assisted vaginal birth rate and APGAR scores in the first minute were lower in women who underwent hydrotherapy. There was no difference between groups in terms of the duration of the first and second stage of labour, episiotomy, perineal trauma, intrapartum and postpartum bleeding amounts, use of pain medication and labour augmentations, APGAR scores in the fifth minute, positive neonatal bacterial culture and neonatal intensive care unit need.ConclusionThis study revealed that the results that hydrotherapy decreased the perception of pain and assisted birth, increased the rate of vaginal birth and comfort level and did not adversely affect the health of the mother and baby during the birth process.
The feasibility trial of nurse‐directed education programme among benign prostatic hyperplasia patients
Authors: Choudhary Dakshta , Kalal Nipin , Kumar Ashok , Choudhary Gautam Ram , Sharma Priya 
Published: August 2023

AbstractDoes nursing directed education have any impact on severity of symptoms and quality of life among BPH patients? This study aims to develop a nurse‐directed education program, to assess and compare the effectiveness of the program on symptoms‐severity and quality of life among BPH patients. BPH, one of the most common causes of Lower Urinary Tract Symptoms (LUTS) in elderly men, is caused by a combination of prostate proliferation and lower urinary tract obstruction. A cross‐sectional study conducted by Divmehar Kaur et al. 2020 at Rajindra Hospital, Patiala, Punjab concluded that BPH patients' quality of life is negatively affected by LUTS associated with BPH (p ≤ 0.001). Also, a field study conducted by Özcan et al. in Turkey concluded that subjects' BPH‐QLS ratings varied statistically significantly (p 0.05) depending on the age, educational attainment, the occurrence of micturition issues, and whether they had ever applied to a medical facility. Then another descriptive study, correlational‐type carried by Pinto et al. in 2014 in Singapore found that patients with BPH had a poor health‐related quality of life and suffered significantly from their psychological wellbeing. And a RCT conducted in Korea by Jung et al. in 2011 with the aim of examining the benefits of tai chi on patients with benign prostatic hypertrophy's (BPH's) lower urinary tract symptoms (LUTSs) and quality of life (QoL) found that tai chi group's change in overall QoL score was more notable (t = 3.06; p = 0.005) and it demonstrated significant improvements. A quasi‐experimental study was conducted among the BPH patients visiting the Urology OPD of AIIMS Jodhpur. Data were collected from 10 BPH patients (5 in each group) using a purposive sampling technique from those over 40 years diagnosed with BPH for the last 6 months after taking informed consent, excluding those being advised for surgery. Self‐structured sociodemographic and clinical variables tool, International Prostate Symptom Score (IPSS), and 12‐item Short Form Survey (SF‐12) were administered. Statistical analysis was performed by employing the SPSS version 23. Chi‐square and Mann–Whitney U tests were used. It was seen that there was a significant difference (p‐value < 0.05) concerning the IPSS‐QOL Index (p‐value = 0.008) of symptoms‐severity (IPSS) and PCS‐12 (p‐value = 0.009) of quality of life scale (SF‐12) between control and experimental groups after 4th‐week post‐intervention (nurse‐directed education program). Findings from this study will help reinforce designing a cost‐effective care delivery system in the care of BPH patients. This program is a novel approach to improving preventive, curative, and rehabilitative services to BPH patients in India.
Neutrophil CD64 index as a good biomarker for early diagnosis of bacterial infection in pregnant women during the flu season
Authors: Yu Lifei , Cen Panpan , Zhang Linjian , Ke Jianfei , Xu Xiangfei , Ding Jiexia , Jin Jie , Leng Jianhang , Yu Yunsong 
Published: August 2023

AbstractBackgroundPregnant women are at high risk of developing febrile illness during the flu season. Early identification of a viral or bacterial infection is crucial in the management of febrile pregnant patients. Neutrophil CD64 (nCD64) has been shown to have more important diagnostic value in sepsis than traditional inflammatory indicators.MethodsThe pregnant women enrolled were divided into three groups according to disease: influenza A infection, bacterial infection and healthy controls. Peripheral blood CD64, leukocyte, C‐reactive protein (CRP), procalcitonin (PCT) and human Th1/Th2‐related cytokines levels were routinely measured. The correlation between and diagnostic value of the nCD64 index and other biomarkers were evaluated using Spearman's correlation test and receiver operating characteristic (ROC) curve analysis.ResultsPregnant women with bacterial infection had significantly elevated levels of leukocytes (8.4 vs. 5.95, 109/L; P = 0.004), CRP (89.70 vs. 50.05 mg/mL; P = 0.031), PCT (0.13 vs. 0.04 ng/mL; P = 0.010) and TNF‐α (0.46 vs. 0.38 pg/mL; P = 0.012) and an elevated nCD64 index (12.16 vs. 0.81; P < 0.001) compared with those with influenza A infection. The area under the receiver operating characteristic (AUROC) curve of the nCD64 index to discriminate bacterial infection among pregnant women (AUROC = 0.9183, P < 0.0001) was the largest. The sensitivity and specificity of the nCD64 index at an optimal cut‐off value of 3.16 were 84% and 100%, respectively, with a negative predictive value (NPV) of 94%.ConclusionsOur study demonstrates the clinical value of the nCD64 index in distinguishing between bacterial infection and influenza A in pregnant women.
Tempo‐spatial evolution of seed plant endemism in Taiwan island
Authors: Ye Jun‐Wei , Yang Zhao‐Zhen , Tian Bin 
Published: August 2023

AbstractAimIn Taiwan island, recent land‐bridge and oversea dispersal after the appearance of proto‐Taiwan (<6.5 Ma) is responsible for its biodiversity assembly. Radiations have also been widely reported in mountain systems. The tempo‐spatial route to the floral hotspot was determined through a meta‐analysis of the evolution of endemic plants.LocationTaiwan island and its adjacent regions.TaxonSeed plants.MethodsPublished dated phylogenies were compiled to infer the origin times represented by the stem ages. To determine the speciation mode, either in situ origin or dispersal origin, the ancestral range shift pattern and/or current distribution range of the sister taxa were used with 10 different biogeographical regions classified. Histograms quantifying the numbers of different origin modes within bins of 0.5 Ma were constructed. Probabilities of long‐distance dispersal (LDD) were evaluated.ResultsThe 125 sampled species originate between the late Eocene and late Pleistocene, of which 14 (11.2%) and 111 (88.8%) were derived before and after the emergence of proto‐Taiwan, respectively. Spatially, in situ speciation would have a bigger contribution to the formation of endemism than dispersal events (56.8% vs. 43.2%). The species with in situ origin (mean = 1.83 Ma) were significantly younger than those with dispersal origin (mean = 6.34 Ma). The main regions where dispersal occurred were South‐Central China (n = 29) and Southeast China (n = 28), followed by Japan (n = 14). High probabilities (60.8%) of LDD were found.Main ConclusionsSpatially, recent land‐bridge and oversea dispersal from the adjacent Asian flora is supported and the southern part of China was found to be the most important source. In situ speciation that may have correlated with mountain uplift, monsoon intensification and Pleistocene climatic fluctuations exerted greater contributions to the biodiversity there. Temporally, floral endemism predominantly occurred after the emergence of proto‐Taiwan. Additional investigations with more sophisticated sampling and genetic data are needed in the future.
Unipolar and bipolar electrograms to predict successful ablation site of premature ventricular contractions originating from the free wall of the tricuspid annulus
Authors: Liu Qi‐Fang , Tian Ye , Tian Long‐Hai , Jing Huang 
Published: August 2023

AbstractIntroductionThis study aimed to identify the characteristics of unipolar and bipolar electrogram (UniEGM and BiEGM) in guiding successful ablation of premature ventricular contractions (PVCs) originating from the free wall of the ventricular aspect of the tricuspid annulus (TA). We hypothesized that the negative concordance pattern (NCP) on the onset of UniEGM and BiEGM, together with the least value of the difference between the earliest BiEGM and UniEGM dV/dTmax, might improve the accuracy of conventional mapping.Methods and ResultsThirty consecutive patients who underwent successful catheter ablation from February 2018 to July 2021 were retrospectively analyzed. The BiEGM and UniEGM for successful ablation sites were compared with those for non‐successful ablation sites. Among the 30 patients, 30 successful and 26 nonsuccessful ablation sites were compared. The earliest activation time of the BiEGM (BiEGMoneset‐QRS) was 25 ± 6 ms for the successful ablation sites and 21 ± 6 ms for the nonsuccessful ablation sites (p = .47). The value of the difference in the earliest BiEGM and UniEGM dV/dTmax differed between successful and nonsuccessful ablation sites (6.4 ± 3.6 ms vs. 10.4 ± 6.8 ms). NCP was observed at 90.0% and 42.3% of the successful and nonsuccessful ablation sites, respectively. Alignment of NCP and BiEGMonset‐UniEGM ≤6 ms was applied as the mapping criterion for successful PVC suppression (73.1% sensitivity and 87.7% specificity). The area under the receiver‐operating characteristic curve for this cutoff was 0.85.ConclusionMapping based on an NCP at the onset of the BiEGM and UniEGM and the least difference value of the earliest BiEGM and UniEGM dV/dTmax had an excellent predictive value for successful ablation. These strategies may reduce the number of radiofrequency catheter ablation (RFCA) applications for free‐wall tricuspid annular PVCs.