bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altitude-induced effects on neuromuscular, metabolic and perceptual responses before, during and after a high-intensity resistance training session
Authors: Márquez Gonzalo , Colomer David , Benavente Cristina , Morenilla Luis , Alix-Fages Carlos , Padial Paulino , Feriche Belén 
Published: May 2023

Abstract
Purpose
We tested if an acute ascending to 2320 m above sea level (asl) affects corticospinal excitability (CSE) and intracortical inhibition (SICI) measured with transcranial magnetic stimulation (TMS) at rest, before, during and after a traditional hypertrophy-oriented resistance training (RT) session. We also explored whether blood lactate concentration (BLa), ratings of perceived exertion (RPE), perceived muscular pain and total training volume differed when the RT session was performed at hypoxia (H) or normoxia (N).

Methods
Twelve resistance-trained men performed eight sets of 10 repetitions at 70% of one repetition maximum of a bar biceps curl at N (SpO2 = 98.0 ± 0.9%) and H (at 2320 asl, SpO2 = 94.0 ± 1.9%) in random order. Before each session, a subjective well-being questionnaire, the resting motor threshold (rMT) and a single pulse recruitment curve were measured. Before, during and after the RT session, BLa, RPE, muscle pain, CSE and SICI were measured.

Results
Before the RT session only the rMT differed between H (− 5.3%) and N (ES = 0.38). RPE, muscle pain and BLa increased through the RT session and were greater at H than N (12%, 54% and 15%, respectively) despite a similar training volume (1618 ± 468 kg vs. 1638 ± 509 kg). CSE was reduced during the RT session (~ 27%) but recovered ten minutes after, regardless of the environmental condition. SICI did not change after any RT session.

Conclusions
The data suggest that acute exposure to moderate hypoxia slightly increased the excitability of the most excitable structures of the corticospinal tract but did not influence intracortical or corticospinal responses to a single RT session.
Postoperative drainage management and wound complications following resection of lower limb soft tissue tumors: a retrospective cohort study
Authors: Gerken A. L. H. , Jawny P. , Weigl H. , Yang C. , Hardt J. , Menge F. , Hohenberger P. , Weiß C. , Reißfelder C. , Jakob J. 
Published: May 2023

Abstract
Purpose
Postoperative wound complications are common in patients undergoing resection of lower extremity soft tissue tumors. Postoperative drainage therapy ensures adequate wound healing but may delay or complicate it. The aim of this study is to evaluate the incidence of postoperative wound complications and delayed or prolonged drainage treatment and to propose a standardized definition and severity grading of complex postoperative courses.

Methods
A monocentric retrospective analysis of 80 patients who had undergone primary resection of lower extremity soft tissue tumors was performed. A new classification was developed, which takes into account postoperative drainage characteristics and wound complications. Based on this classification, risk factors and the prognostic value of daily drainage volumes were evaluated.

Results
According to this new definition, regular postoperative course grade 0 (no wound complication and timely drainage removal) occurred in 26 patients (32.5%), grade A (minor wound complications or delayed drainage removal) in 12 (15.0%), grade B (major wound complication or prolonged drainage therapy) in 31 (38.8%), and grade C (reoperation) in 11 (13.7%) patients. Tumor-specific characteristics, such as tumor size (p = 0.0004), proximal tumor location (p = 0.0484), and tumor depth (p = 0.0138) were identified as risk factors for complex postoperative courses (grades B and C). Drainage volume on postoperative day 4 was a suitable predictor for complex courses (cutoff of 70 ml/d).

Conclusion
The proposed definition incorporates wound complications and drainage management while also being clinically relevant and easy to apply. It may serve as a standardized endpoint for assessing the postoperative course after resection of lower extremity soft tissue tumors.
Parafoveal syntactic processing from word N + 2 during reading: the case of gender-specific German articles
Authors: Schwalm Laura , Radach Ralph 
Published: May 2023

AbstractPrevious research has suggested that some syntactic information such as word class can be processed parafoveally during reading. However, it is still unclear to what extent early syntactic cueing within noun phrases can facilitate word processing during dynamic reading. Two experiments (total N = 72) were designed to address this question using a gaze-contingent boundary change paradigm to manipulate the syntactic fit within a nominal phrase. Either the article (Experiment 1) or the noun (Experiment 2) was manipulated in the parafovea, resulting in a syntactic mismatch, depending on the condition. Results indicated a substantial elevation of viewing times on both parts of the noun phrase when conflicting syntactic information had been present in the parafovea. In Experiment 1, the article was also fixated more often in the syntactic mismatch condition. These results provide direct evidence of parafoveal syntactic processing. Based on the early time-course of this effect, it can be concluded that grammatical gender is used to generate constraints for the processing of upcoming nouns. To our knowledge, these results also provide the first evidence that syntactic information can be extracted from a parafoveal word N + 2.
Exome sequencing of choreoacanthocytosis reveals novel mutations in VPS13A and co-mutation in modifier gene(s)
Authors: Chaudhari Sima , Ware Akshay Pramod , Jasti Dushyanth Babu , Gorthi Sankar Prasad , Acharya Lavanya Prakash , Bhat Manoj , Mallya Sandeep , Satyamoorthy Kapaettu 
Published: May 2023

AbstractChoreoacanthocytosis, one of the forms of neuroacanthocytosis, is caused by mutations in vacuolar protein sorting-associated protein A (VPS13A), and is often misdiagnosed with other form of neuroacanthocytosis with discrete genetic defects. The phenotypic variations among the patients with VPS13A mutations significantly obfuscates the understanding of the disease and treatment strategies. In this study, two unrelated cases were identified, exhibiting the core phenotype of neuroacanthocytosis but with considerable clinical heterogeneity. Case 1 presented with an additional Parkinsonism phenotype, whereas seizures were evident in case 2. To decipher the genetic basis, whole exome sequencing followed by validation with Sanger sequencing was performed. A known homozygous pathogenic nonsense mutation (c.799C > T; p.R267X) in exon 11 of the VPS13A gene was identified in case 1 that resulted in a truncated protein. A novel missense mutation (c.9263T > G; p.M3088R) in exon 69 of VPS13A identified in case 2 was predicted as pathogenic. In silico analysis of the p.M3088R mutation at the C-terminus of VPS13A suggests a loss of interaction with TOMM40 and may disrupt mitochondrial localization. We also observed an increase in mitochondrial DNA copy numbers in case 2. Mutation analysis revealed benign heterozygous variants in interacting partners of VPS13A such as VAPA in case 1. Our study confirmed the cases as ChAc and identified the novel homozygous variant of VPS13A (c.9263T > G; p.M3088R) within the mutation spectrum of VPS13A-associated ChAc. Furthermore, mutations in VPS13A and co-mutations in its potential interacting partner(s) might contribute to the diverse clinical manifestations of ChAc, which requires further study.
Adopting the Rumsfeld approach to understanding the action of levodopa and apomorphine in Parkinson’s disease
Authors: Jenner P. , Falup-Pecurariu C. , Leta V. , Verin M. , Auffret M. , Bhidayasiri Roongroj , Weiss D. , Borovečki F. , Jost W. H. 
Published: May 2023

AbstractDopaminergic therapies dominate the treatment of the motor and non-motor symptoms of Parkinson’s disease (PD) but there have been no major advances in therapy in many decades. Two of the oldest drugs used appear more effective than others—levodopa and apomorphine—but the reasons for this are seldom discussed and this may be one cause for a lack of progress. This short review questions current thinking on drug action and looks at whether adopting the philosophy of ex-US Secretary of State Donald Rumsfeld reveals ‘unknown’ aspects of the actions of levodopa and apomorphine that provide clues for a way forward. It appears that both levodopa and apomorphine have a more complex pharmacology than classical views would suggest. In addition, there are unexpected facets to the mechanisms through which levodopa acts that are either forgotten as ‘known unknowns’ or ignored as ‘unknown unknowns’. The conclusion reached is that we may not know as much as we think about drug action in PD and there is a case for looking beyond the obvious.
Single-cell RNA sequencing and kidney organoid differentiation
Authors: Uchimura Kohei 
Published: May 2023

AbstractSince 2015, Japanese researchers have made great progress in developing a method to differentiate human pluripotent stem cells (hPSCs) into kidney organoids. Protocols have been established to produce increasingly complex three-dimensional (3D) structures, which are used as a human kidney disease model and adapted for high-throughput screening. During this period, single-cell RNA sequencing (scRNA-seq) technology was developed to perform a comprehensive analysis at the single-cell level. We have performed a comprehensive analysis using scRNA-seq to define how kidney organoids can be applied to understand kidney development and pathology. The structure of kidney organoids is complex and contains many cell types of varying maturity. Since only a few proteins and mRNAs can be identified by immunostaining and other techniques, we performed scRNA-seq, which is an unbiased technology that can comprehensively categorize all cell types present in organoids. The aim of this study is to review the problems of kidney organoids based on scRNA-seq and the efforts to address the problems and predict future applications with this powerful technique.
Evaluation of Hydrotalcites Produced in Seawater for Remediation of Metalliferous Acid Mine Drainage and Submarine Placement
Authors: Suliartini Ni Made Sri , Joll Cynthia A. , Douglas Grant B. 
Published: May 2023

AbstractUncontrolled release of acid mine drainage (AMD) causes widespread detrimental impacts on the receiving environment. Thus, effective treatment to neutralise AMD effluent pH and capture a suite of metals is required. In-situ hydrotalcite (HTC) precipitation is an emerging technology for AMD remediation. HTC has an inherent capacity to accommodate a range of cations and anions during in situ formation, offering a method of broad-spectrum contaminant removal. This study explored the feasibility of using seawater as an Mg source and synthetic AMD in HTC formation. The HTC was formed from a stoichiometric combination of synthetic AMD and seawater. While three initial stoichiometric M2+:M3+ ratios of 2:1, 3:1, and 4:1 were investigated, only HTC with an M2+:M3+ ratio of 2:1 was generated, as confirmed by both mineralogical and geochemical analyses. Importantly, the HTC was demonstrated to effectively remove a suite of metals present in AMD such as Cu, Zn, Al, and Mn with removal rates of between 99.97 to 99.99%. The HTC precipitate contained ≈6.6% Cu and 4.1% Zn, and thus shows the potential, if required, for future metal recovery. Since submarine placement is often used in metal mining and processing operations proximal to the coast, the stability of the HTC precipitate in seawater was also investigated. Importantly, only 0.2% of the Cu and 1.1% of the Zn within the HTC were subsequently leaching in decreasing increments into seawater over 30 days with decreasing increments after the initial seven days. This indicates robust element retention and confirms the potential of HTC for AMD remediation with direct submarine placement.
In vivo quantitative MRI: T1 and T2 measurements of the human brain at 0.064 T
Authors: Jordanova Kalina V. , Martin Michele N. , Ogier Stephen E. , Poorman Megan E. , Keenan Kathryn E. 
Published: May 2023

Abstract
Objective
To measure healthy brain $${T}_{1}$$

T
1

and $${T}_{2}$$

T
2

relaxation times at 0.064 T.

Materials and methods
$${T}_{1}$$

T
1

and $${T}_{2}$$

T
2

relaxation times were measured in vivo for 10 healthy volunteers using a 0.064 T magnetic resonance imaging (MRI) system and for 10 test samples on both the MRI and a separate 0.064 T nuclear magnetic resonance (NMR) system. In vivo $${T}_{1}$$

T
1

and $${T}_{2}$$

T
2

values are reported for white matter (WM), gray matter (GM), and cerebrospinal fluid (CSF) for automatic segmentation regions and manual regions of interest (ROIs).

Results
$${T}_{1}$$

T
1

sample measurements on the MRI system were within 10% of the NMR measurement for 9 samples, and one sample was within 11%. Eight $${T}_{2}$$

T
2

sample MRI measurements were within 25% of the NMR measurement, and the two longest $${T}_{2}$$

T
2

samples had more than 25% variation. Automatic segmentations generally resulted in larger $${T}_{1}$$

T
1

and $${T}_{2}$$

T
2

estimates than manual ROIs.

Discussion
$${T}_{1}$$

T
1

and $${T}_{2}$$

T
2

times for brain tissue were measured at 0.064 T. Test samples demonstrated accuracy in WM and GM ranges of values but underestimated long $${T}_{2}$$

T
2

in the CSF range. This work contributes to measuring quantitative MRI properties of the human body at a range of field strengths.
When it rains it pours? A time-series approach to the relationship between homicide and other adverse health phenomena
Authors: van Breen Jolien , Liem Marieke 
Published: May 2023

Abstract
Aim
This paper aims to better understand the relationship between homicide and other public health outcomes, by studying their trends over time.

Subject and methods
Research in both criminology and public health has long identified that crime and violence tend to cluster together with adverse phenomena in the social and health domains. However, such work has relied primarily on cross-sectional analyses. Here, we instead study trends over time. We take data from the Netherlands, between 2000 and 2020, and ask whether homicide shows similar trends over time as other public health phenomena – such as smoking behaviour, alcohol use, child mortality, adolescent pregnancies, and suicide.

Results
We observe, first, that all of the phenomena – with the exception of suicide – declined over the period under study. We then employ a time series analysis to examine whether these trends arise independently, or whether they are the result of structural similarities between phenomena. Results showed that the decline in homicide rates is linked to a similar decline in adolescent pregnancies – the rates of these phenomena ‘move together’ during the period under study.

Conclusion
This work shows that the phenomenon of homicide shares structural similarities with teenage pregnancies – a decline in one is linked to a similar decline in the other. More generally, the current work furthers our understanding of the place of homicide in the domain of (public) health.
Fabrication of polysulfone membranes by blending with polyaniline and cellulose nanocrystals: towards the effective separation of oil-in-water emulsions
Authors: Fahmy Hanan S. , Abouzeid Ragab , El-sadek M. S. Abd , Abdel-Jaber G. T. , Ali W. Y. , Mousa Hamouda M. 
Published: May 2023

AbstractIn the present work, we developed a hybrid membrane via phase inversion process composed from polysulfone (PSF), cellulose nanocrystals (CNCs), and polyaniline (PANI) polymers for oil/water separation. Multiple techniques were used to characterize the developed membranes. Results demonstrated that under a gravity-driven process, the blended PSF membrane with CNC and PANI had a greatly higher water flux of 31.10, 29.01 and 29.95 L m−2 h−1 for pure water, soybean water mixture, and diesel water mixture respectively as compared to pure PSF and PSF/CNC membranes. Meanwhile, the water flux values under constant pressure-driven process, high water flux of PSF/CNC/PANI membrane could reach 800 L m−2 h−1 KPa−1 for various types of oils. The results showed ultrafiltration (UF) membrane with good micro-porosity, acceptable hydrophilicity, and good mechanical properties. Moreover, PSF/CNC/PANI membrane possessed high permeability properties at gravity-driven and pressure-driven processes for various oil water mixture separation with oil rejection efficiency (~ 88.2%).
Primary tumor resection or systemic treatment as palliative treatment for patients with isolated synchronous colorectal cancer peritoneal metastases in a nationwide cohort study
Authors: Rijken Anouk , van de Vlasakker Vincent C. J. , Simkens Geert A. , Rovers Koen P. , van Erning Felice N. , Koopman Miriam , Verhoef Cornelis , de Wilt Johannes H. W. , de Hingh Ignace H. J. T. 
Published: May 2023

AbstractLimited data are available to guide the decision-making process for clinicians and their patients regarding palliative treatment options for patients with isolated synchronous colorectal cancer peritoneal metastases (CRC-PM). Therefore, the aim of this study is to analyze the outcome of the different palliative treatments for these patients. All patients diagnosed with isolated synchronous CRC-PM between 2009 and 2020 (Netherlands Cancer Registry) who underwent palliative treatment were included. Patients who underwent emergency surgery or curative intent treatment were excluded. Patients were categorized into upfront palliative primary tumor resection (with or without additional systemic treatment) or palliative systemic treatment only. Overall survival (OS) was compared between both groups and multivariable cox regression analysis was performed. Of 1031 included patients, 364 (35%) patients underwent primary tumor resection and 667 (65%) patients received systemic treatment only. Sixty-day mortality was 9% in the primary tumor resection group and 5% in the systemic treatment group (P = 0.007). OS was 13.8 months in the primary tumor resection group and 10.3 months in the systemic treatment group (P < 0.001). Multivariable analysis showed that primary tumor resection was associated with improved OS (HR 0.68; 95%CI 0.57–0.81; P < 0.001). Palliative primary tumor resection appeared to be associated with improved survival compared to palliative systemic treatment alone in patients with isolated synchronous CRC-PM despite a higher 60-day mortality. This finding must be interpreted with care as residual bias probably played a significant role. Nevertheless, this option may be considered in the decision-making process by clinicians and their patients.
Preschool children’s conceptions of the meanings and use of written numerals in everyday life: a phenomenographic study of the nature and structure of qualitative variation
Authors: Voutsina Chronoula , Stott Debbie 
Published: May 2023

AbstractSupporting children’s understanding of the everyday, cultural use of written numerals is highly significant, as it is this understanding that gives meaning to classroom conversations on the purposes of written numbers. This paper presents findings from a phenomenographic study of the qualitatively different ways in which 3–5-year-old children interpret the meanings and use of numerals in everyday contexts. The study involved a volunteer sample of 37 preschool children. With their family’s support, children played a Number Spotting game, taking photographs of numerals in their environments. These photographs were supplemented with other photographs selected by the researchers and used in individual photo-elicitation interviews with children. We collected data on children’s interpretations of a range of examples of numerals used to denote quantity, order and measurement, and numerals used as labels/identifiers. The findings document qualitatively different categories that capture the range of children’s expressed conceptions as well as the critical aspects of variation that underpin how qualitatively different categories of conceptions differ or relate to each other. The study provides original insights into the nature and structure of children’s awareness of the cultural uses of written numerals. The findings can support early mathematics teaching to make meaningful connections between the knowledge that children develop outside of school and the new knowledge about written numbers that they develop in formal education.
Peracetic acid as a disinfectant for wastewater reuse — Regulation (EU) 2020/741 application on a pilot-scale
Authors: Dias Rita , Sousa Diogo , Lourinho Rita , Maurício Rita 
Published: May 2023

AbstractWater scarcity affects already a large part of the world's population. To overcome this situation, water management is needed, and wastewater reuse must be implemented and included as a new approach. To achieve that objective water quality must comply with the parameters established in the Regulation (EU) 2020/741 of the European Parliament and the Council of the European Union and new treatment solutions have to be developed. The main goal of this pilot study was to evaluate the peracetic acid (PAA) disinfection efficiency in a real wastewater treatment plant (WWTP) in order to accomplish the wastewater reuse objective. To this end, six disinfection conditions were studied, three PAA doses (5, 10, and 15) and three contact times (5, 10, and 15) based on the commonly used disinfection operational conditions in real WWTP. Comparing the Total Suspended Solids (TSS), turbidity, Biological Oxygen Demand (BOD5) and Escherichia coli content, after and before the disinfection step, was possible to conclude that PAA ensures the Regulation (EU) 2020/741 requirements and that the disinfected effluent can be reused for several uses. All the conditions in which the PAA dose was 15 mg/L and the condition with 10 mg/L of PAA with a contact time of 15 min were the most promising, presenting the second highest water quality class achieved. The results of this study illustrate the potential of PAA as an alternative disinfectant for wastewater treatment and, bring it closer to the water reuse objective by presenting several possibilities for water uses.
Sām.ṃkhya’s Challenge to the Buddhist Claim of the Identity of a Pramān.ṇa and Its Result
Authors: Łucyszyna Ołena 
Published: May 2023

AbstractSāṃkhya, in its commentary Yuktidīpikā, responds to the Buddhist claim that a means of valid cognition (pramāṇa) and a valid cognition (pramā), its result (phala), are identical. The response of Sāṃkhya was pioneering: it is one of the two earliest responses to the Buddhists in the lively polemic on the relationship between a pramāṇa and its result. (The other of these two earliest responses is in the Ślokavārttika by Kumārila Bhaṭṭa.) Sāṃkhya’s voice in this polemic is earlier than that of Nyāya, which is, as well as Mīmāṃsā, the main rival of the Buddhists in addressing this issue. This study provides a translation and detailed reconstruction of the Yuktidīpikā’s polemic with the Buddhist opponent, which has not been researched before, as well as a critical assessment of the Sāṃkhya position. The Yuktidīpikā polemicizes against Dignāga. It aptly questions the standpoint of the Buddhist opponent and presents an alternative standpoint, contrasting its own view with that of the opponent. Though the Sāṃkhya position formulated in the Yuktidīpikā evokes several critical remarks, the Yuktidīpikā’s response is an important contribution to Indian thought.