bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo
Authors: Kozak Eva L. , Miranda-Rodríguez Jerónimo R. , Borges Augusto , Dierkes Kai , Mineo Alessandro , Pinto-Teixeira Filipe , Viader-Llargués Oriol , Solon Jérôme , Chara Osvaldo , López-Schier Hernán 
Published: March 2023

ABSTRACT
Collective cell rotations are widely used during animal organogenesis. Theoretical and in vitro studies have conceptualized rotating cells as identical rigid-point objects that stochastically break symmetry to move monotonously and perpetually within an inert environment. However, it is unclear whether this notion can be extrapolated to a natural context, where rotations are ephemeral and heterogeneous cellular cohorts interact with an active epithelium. In zebrafish neuromasts, nascent sibling hair cells invert positions by rotating ≤180° around their geometric center after acquiring different identities via Notch1a-mediated asymmetric repression of Emx2. Here, we show that this multicellular rotation is a three-phasic movement that progresses via coherent homotypic coupling and heterotypic junction remodeling. We found no correlation between rotations and epithelium-wide cellular flow or anisotropic resistive forces. Moreover, the Notch/Emx2 status of the cell dyad does not determine asymmetric interactions with the surrounding epithelium. Aided by computer modeling, we suggest that initial stochastic inhomogeneities generate a metastable state that poises cells to move and spontaneous intercellular coordination of the resulting instabilities enables persistently directional rotations, whereas Notch1a-determined symmetry breaking buffers rotational noise.
Preservation of heritage buildings in Alexandria, Egypt: an application of heritage digitisation process phases and new documentation methods
Authors: El Menshawy Adel , Omar Walid , El Adawy Sherif 
Published: March 2023

Background: Throughout the history of the city, the architecture of Alexandria, Egypt, has been in contact with world cultures, especially those of the Mediterranean sphere. Alexandria is rich with cultural features dating back seven thousand years. In Alexandria, the heritage value of the city has decreased since the beginning of the third millennium of the Common Era because there is no suitable digital documentation system for these more recent assets. The development of a new technique for preserving heritage buildings is required. For example, image- based techniques can gather data using photography, panoramic photography, and close-range photogrammetry. In this research, we primarily seek to implement Heritage Digitisation Process Phases (HDPP) by introducing both the Building Information Modelling (BIM) environment and the point clouds for achieving a Historic Building Information Modelling (HBIM) model and to establish new documentation methods in architectural conservation and built-heritage preservation, i.e., Virtual Reality (VR) and Website Heritage Documentation (WHD). Methods: The methodology is designed to preserve and manage cultural heritage using HDPP for the promotion of heritage building preservation in Alexandria. Results: The results show that the application of HDPP has led to the creation of a digital database about the Société Immobilière building, which was chosen as a case study for this research. Conclusions: Implementation of HDPP and usage of new documentation methods i.e., VR and WHD create a digital path to help strengthen its image and connect the place to users, recreational areas are created to communicate and explore the city’s architectural history.
Restinga ectomycorrhizae: a work in progress
Authors: N. M. Furtado Ariadne , Leonardi Marco , Comandini Ornella , Neves Maria Alice , C. Rinaldi Andrea 
Published: March 2023

Background: The Brazilian Atlantic Forest is one of the most biodiverse terrestrial ecoregions of the world. Among its constituents, restinga vegetation makes a particular case, acting as a buffer zone between the oceans and the forest. Covering some 80% of Brazilian coastline (over 7,300 km in length), restinga is a harsh environment where plants and fungi interact in complex ways that just now are beginning to be unveiled. Ectomycorrhizal symbiosis, in particular, plays a so far ungauged and likely underestimated role. We recently described the morpho-anatomical and molecular features of the ectomycorrhizae formed by several basidiomycetous mycobionts on the host plant Guapira opposita, but the mycorrhizal biology of restinga is still largely unexplored. Here, we report new data on the ectomycorrhizal fungal symbionts of G. opposita, based on the collection of sporomata and ectomycorrhizal root tips in restinga stands occurring in southern Brazil. Methods: To obtain a broader view of restinga mycorrhizal and ecological potential, we compiled a comprehensive and up-to-date checklist of fungal species reported or supposed to establish ectomycorrhizae on restinga-inhabiting host plants, mainly on the basis of field observations. Results: Our list comprises some 726 records, 74 of which correspond to putative ectomycorrhizal taxa specifically associated with restinga. These include several members of Boletaceae, Amanita, Tomentella/Thelephora, Russula/Lactifluus, and Clavulina, as well as hypogeous fungi, like the recently described Longistriata flava. Conclusions: Our survey reveals a significant diversity of the restinga ectomycorrhizal mycobiota, indicating the importance of this symbiosis for the ecological functioning of a unique yet poorly known and threatened ecosystem.
Comparative study on scenarios for rotating gantry mechanical structures
Authors: Piacentini Luca , Dassa Luca , Perini Diego , Ratkus Andris , Torims Toms , Uberti Stefano , Vilcans Janis , Vretenar Maurizio 
Published: March 2023

Background: This paper outlines a comparative study on scenarios of gantries mechanical design for ion-therapy of cancer, which is a crucial step toward the development of the next ion medical machine. Methods: Multiple scenarios were considered based on robustness of the design, size, weight and complexity, deformation and precision performances and costs, as well as environmental impact. Four prospective scenarios were identified, each of them capable of providing beam to at least 220° around the patient. One scenario is capable of providing treatment angles of 360°. This report will describe the unified methodology performed during the study in order to achieve unbiased results in a comprehensive manner. Results: Results show that in statically balanced scenarios, considerable improvements can be reached in terms of safety, deformation, precision performances, complexity and costs of implementation. Conclusions: All scenarios are deemed suitable for further gantry design development.
Mixed-Integer Programming vs. Constraint Programming for Shop Scheduling Problems: New Results and Outlook
Authors: Naderi Bahman , Ruiz Rubén , Roshanaei Vahid 
Published: March 2023

Constraint programming (CP) has been recently in the spotlight after new CP-based procedures have been incorporated into state-of-the-art solvers, most notably the CP Optimizer from IBM. Classical CP solvers were only capable of guaranteeing the optimality of a solution, but they could not provide bounds for the integer feasible solutions found if interrupted prematurely due to, say, time limits. New versions, however, provide bounds and optimality guarantees, effectively making CP a viable alternative to more traditional mixed-integer programming (MIP) models and solvers. We capitalize on these developments and conduct a computational evaluation of MIP and CP models on 12 select scheduling problems. 1 We carefully chose these 12 problems to represent a wide variety of scheduling problems that occur in different service and manufacturing settings. We also consider basic and well-studied simplified problems. These scheduling settings range from pure sequencing (e.g., flow shop and open shop) or joint assignment-sequencing (e.g., distributed flow shop and hybrid flow shop) to pure assignment (i.e., parallel machine) scheduling problems. We present MIP and CP models for each variant of these problems and evaluate their performance over 17 relevant and standard benchmarks that we identified in the literature. The computational campaign encompasses almost 6,623 experiments and evaluates the MIP and CP models along five dimensions of problem characteristics, objective function, decision variables, input parameters, and quality of bounds. We establish the areas in which each one of these models performs well and recognize their conceivable reasons. The obtained results indicate that CP sets new limits concerning the maximum problem size that can be solved using off-the-shelf exact techniques. History: Accepted by Pascal Van Hentenryck, Area Editor for Computational Modeling: Methods & Analysis. Supplemental Material: The software that supports the findings of this study is available within the paper and its Supplemental Information ( https://pubsonline.informs.org/doi/suppl/10.1287/ijoc.2023.1287 ) as well as from the IJOC GitHub software repository ( https://github.com/INFORMSJoC/2021.0326 ) at ( http://dx.doi.org/10.5281/zenodo.7541223 ).
Liquidity Requirements and Central Bank Interventions During Banking Crises
Authors: Robatto Roberto 
Published: March 2023

This paper evaluates liquidity requirements and public liquidity injections in the context of financial crises, using a model that includes near-money assets. Some key effects are transmitted through liquidity premia and the price of liquid assets, producing a heterogeneous impact that depends on an agent’s holdings of liquid assets. In addition, some agents prefer policies that do not fully relax their liquidity constraints because these policies are associated with higher prices. Liquidity requirements and liquidity injections have the opposite impact on liquidity premia and combining them leads to Pareto improvements that cannot be achieved by each policy separately. This paper was accepted by Gustavo Manso, finance. Funding: This work was supported by the Wisconsin Alumni Research Foundation. Supplemental Material: The data files are available at https://doi.org/10.1287/mnsc.2023.4737 .
Heatmap-Based Decision Support for Repositioning in Ride-Sharing Systems
Authors: Haferkamp Jarmo , Ulmer Marlin W. , Ehmke Jan Fabian 
Published: March 2023

In ride-sharing systems, platform providers aim to distribute the drivers in the city to meet current and potential future demand and to avoid service cancellations. Ensuring such distribution is particularly challenging in the case of a crowdsourced fleet, as drivers are not centrally controlled but are free to decide where to reposition when idle. Thus, providers look for alternative ways to ensure a vehicle distribution that benefits users, drivers, and the provider. We propose an intuitive mean to improve idle ride-sharing vehicles’ repositioning: repositioning heatmaps. These heatmaps highlight driver-specific earning opportunities approximated based on the expected future demand, current and expected future fleet distribution, and the location of the specific driver. Based on the heatmaps, drivers make decentralized yet better-informed repositioning decisions. As our heatmap policy changes the driver distribution in the future, we propose an adaptive learning algorithm for designing our heatmaps in large-scale ride-sharing systems. We simulate the system and generate heatmaps based on the previously learned policy in every iteration. We then update the policy based on the simulation’s outcome and use it in the next iteration. We test our heatmap design in a comprehensive case study on New York ride-sharing data. We show that carefully designed heatmaps reduce service cancellations and therefore, revenue loss for the platform and drivers significantly while leading to a better service level for the users and to a fairer treatment of drivers. History: This paper has been accepted for the Transportation Science Special Issue on Machine Learning Methods and Applications in Large-Scale Route Planning Problems. Funding: This research is funded by the German Research Foundation (Deutsche Forschungsgemeinschaft) [Grant 494812908]. M. W. Ulmer’s work is funded by the German Research Foundation Emmy Noether Programme [Grant 444657906]. Supplemental Material: The online appendix is available at https://doi.org/10.1287/trsc.2023.1202 .
Spectral and RGB analysis of the light climate and its ecological impacts using an all-sky camera system in the Arctic
Authors: Grant Stephen , Johnsen Geir , McKee David , Zolich Artur , Cohen Jonathan H. 
Published: March 2023

The ArcLight observatory provides an hourly continuous time series of
all-sky images providing light climate data (intensity, spectral
composition, and photoperiod) from the Arctic (Svalbard at 79°N).
Until recently, no complete annual time series of light climate
relevant for biological processes has been provided from the high
Arctic because of insufficient sensitivity of commercial light sensors
during the Polar Night. The ArcLight set up is unique, as it provides
both all-sky images and the corresponding integrated spectral
irradiance in the visible part of the solar electromagnetic spectrum (EPAR). Here we present a further
development providing hourly diel-annual dynamics from 2020 of the
irradiance partitioned into the red, green, and blue parts of the
solar spectrum and illustrate their relation to weather conditions,
and sun and moon trajectories. We show that there is variation between
the RGB proportions of irradiance throughout the year, with the blue
part of the spectrum showing the greatest variation, which is
dependent on weather conditions (i.e., cloud cover). We further
provide an example of the biological impact of these spectral
variations in the light climate using in
vivo Chl a-specific absorption coefficients of diatoms (mean
of six low light acclimated northern-Arctic bloom-forming species) to
model total algal light absorption (AQ
t
o
t
a
l
) and the corresponding fraction of
quanta used by Photosystem II (AQPSII) (O2 production) in RGB bands and the
potential impacts on the photoreceptor response, suggesting periods
where repair and maintenance functions dominate activity in the
absence of appreciable levels of red or green light. The method used
here can be applied to light climate data and spectral response data
worldwide to give localized ecological models of AQ.
Deep ultraviolet high-resolution microscopic hyperspectral imager and its biological tissue detection
Authors: Yang Jingyao , Xue Qingsheng , Li Jinze , Han Bing , Wang Youpeng , Bai Haoxuan 
Published: March 2023

Ultraviolet (UV) hyperspectral imaging technology is commonly used in the field of
atmospheric remote sensing. In recent years, some in-laboratory
research has been carried out for substance detection and
identification. In this paper, UV hyperspectral imaging technology is
introduced into microscopy to better utilize the obvious absorption
characteristics of components, such as proteins and nucleic acids in
biological tissues in the ultraviolet band. A deep UV microscopic
hyperspectral imager based on the Offner structure with F# 2.5, low spectral keystone and smile
is designed and developed. A 0.68 numerical aperture microscope
objective is designed. The spectral range of the system is from 200 nm
to 430 nm; the spectral resolution is better than 0.5 nm; and the
spatial resolution is better than 1.3 µm. The K562 cells can be
distinguished by transmission spectrum of nucleus. The UV microscopic
hyperspectral image of the unstained mouse liver slices showed similar
results to the microscopic image after hematoxylin and eosin staining,
which could help to simplify the pathological examination process.
Both results show a great performance in spatial and spectral
detecting capabilities of our instrument, which has the potential for
biomedical research and diagnosis.
Clinical and lifestyle patterns in Asian children with inflammatory bowel disease in the U.S.
Authors: Zong Wenjing , Patel Amit , Chang Vivian , Mitchel Elana B. , Stoner Natalie , Baldassano Robert N. , Albenberg Lindsey 
Published: March 2023

Background
While there are many epidemiologic studies of Asian immigrants to the West and risk of inflammatory bowel disease (IBD), the phenotype and lifestyle of Asian patients, particularly children, with IBD are not well described. In this study, we describe lifestyle practices, such as dietary pattern, as well as disease phenotype in Asian American children with IBD.

Methods
We reviewed the records of children with IBD, ages 0 to 21 years old, and race identified as Asian, Indian, or Pacific Islander. Patients who received outpatient IBD care at our center between January 2013 and January 2020 were included. We excluded patients who were international second opinions, who did not have a definitive diagnosis of IBD, and in whom a diagnosis of IBD was made after 18 years of age. A survey, including a food frequency questionnaire adapted from NHANES DSQ with modifications to include culturally appropriate food elements, was designed and conducted within this cohort to assess for dietary patterns.

Results
Asian patients in our cohort have similar phenotypes as non-Asians with few distinctive differences. There was a Crohn’s disease and male predominance similar with non-Asians. However, there was a high rate of proctitis in ulcerative colitis in Asian patients. Asian patients reported a typical dietary pattern that reflects a Westernized pattern rather than a traditional pattern. Despite a similar dietary pattern, there was a high rate of 25-OH Vitamin D deficiency (44%) and insufficiency (40%).

Conclusions
This single center study showed that the phenotype of Asian children with IBD in the U.S. is similar with that of non-Asian with a few distinct differences. The Asian children in our cohort reported following a Westernized dietary pattern and lifestyle. However, there was a high rate of Vitamin D deficiency surrounding diagnosis, suggesting a need for vigilant monitoring.
A conceptual model on caregivers’ hesitancy of topical fluoride for their children
Authors: Chi Donald L. , Kerr Darragh , Patiño Nguyen Daisy , Shands Mary Ellen , Cruz Stephanie , Edwards Todd , Carle Adam , Carpiano Richard , Lewis Frances 
Published: March 2023

Background
Topical fluoride hesitancy is a well-documented and growing public health problem. Despite extensive evidence that topical fluoride is safe and prevents tooth decay, an increasing number of caregivers are hesitant about their children receiving topical fluoride, leading to challenges in clinical settings where caregivers refuse preventive care.

Purpose
To explore the determinants of topical fluoride hesitancy for caregivers with dependent children.

Methods
In this qualitative study, we interviewed 56 fluoride-hesitant caregivers to develop an inductive conceptual model of reasons why caregivers are hesitant.

Results
The core construct of the conceptual model of topical fluoride hesitancy centered on caregivers “wanting to protect and not mess up their child”. Six domains comprised this core construct: thinking topical fluoride is unnecessary, wanting to keep chemicals out of my child’s body, thinking fluoride is harmful, thinking there is too much uncertainty about fluoride, feeling pressured to get topical fluoride, and feeling fluoride should be a choice.

Conclusions
Topical fluoride hesitancy is complex and multifactorial. Study findings provide insight for future efforts to understand and optimize caregivers’ preventive care decision making.
Pneumolysin as a target for new therapies against pneumococcal infections: A systematic review
Authors: Cima Cabal María Dolores , Molina Felipe , López-Sánchez José Ignacio , Pérez-Santín Efrén , del Mar García-Suárez María 
Published: March 2023

BackgroundThis systematic review evaluates pneumolysin (PLY) as a target for new treatments against pneumococcal infections. Pneumolysin is one of the main virulence factors produced by all types of pneumococci. This toxin (53 kDa) is a highly conserved protein that binds to cholesterol in eukaryotic cells, forming pores that lead to cell destruction.MethodsThe databases consulted were MEDLINE, Web of Science, and Scopus. Articles were independently screened by title, abstract, and full text by two researchers, and using consensus to resolve any disagreements that occurred. Articles in other languages different from English, patents, cases report, notes, chapter books and reviews were excluded. Searches were restricted to the years 2000 to 2021. Methodological quality was evaluated using OHAT framework.ResultsForty-one articles describing the effects of different molecules that inhibit PLY were reviewed. Briefly, the inhibitory molecules found were classified into three main groups: those exerting a direct effect by binding and/or blocking PLY, those acting indirectly by preventing its effects on host cells, and those whose mechanisms are unknown. Although many molecules are proposed as toxin blockers, only some of them, such as antibiotics, peptides, sterols, and statins, have the probability of being implemented as clinical treatment. In contrast, for other molecules, there are limited studies that demonstrate efficacy in animal models with sufficient reliability.DiscussionMost of the studies reviewed has a good level of confidence. However, one of the limitations of this systematic review is the lack of homogeneity of the studies, what prevented to carry out a statistical comparison of the results or meta-analysis.ConclusionA panel of molecules blocking PLY activity are associated with the improvement of the inflammatory process triggered by the pneumococcal infection. Some molecules have already been used in humans for other purposes, so they could be safe for use in patients with pneumococcal infections. These patients might benefit from a second line treatment during the initial stages of the infection preventing acute respiratory distress syndrome and invasive pneumococcal diseases. Additional research using the presented set of compounds might further improve the clinical management of these patients.