bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping first to final uses for rare earth elements, globally and in the United States
Authors: Alonso Elisa , Pineault David G. , Gambogi Joseph , Nassar Nedal T. 
Published: December 2022

AbstractEstimating the material flows of rare earth elements (REEs) is essential to understanding which industries are most vulnerable to potential REE supply disruptions which, in turn, may inform policy recommendations aimed at reducing the supply risk. However, the REEs are a group of mineral commodities characterized by highly uncertain estimates of supply and demand due to the REE market's complexity, opacity, and small size. In this study, a streamlined methodology was applied to map mineral commodity first‐use to final‐use applications and to estimate total requirements at the national level based on available industrial data for final‐use finished goods. This analysis examines REEs both as a group and individually, showing that total US requirements are between 15% and 16.5% of world requirements for the year 2015, the latest year with the most complete information available. The findings shed light on US industrial capabilities by revealing the discrepancy between the types of REEs that go into US raw material consumption and those that are contained in embedded consumption. For instance, given the United States’ large oil refining industry, US raw material consumption of lanthanum is quite high. In contrast, US raw material consumption of neodymium is relatively low, whereas embedded demand is comparatively high. This reflects the lack of industrial capacity to process REE concentrates into magnet material combined with the US's high imports of products that contain rare earth permanent magnets.
Deletion of Bak1 alleviates microglial necroptosis and neuroinflammation after experimental subarachnoid hemorrhage
Authors: Qiu Xiancheng , Tao Qianke , Zhang Lihan , Kuang Chenghao , Xie Yuke , Zhang Lifang , Yin Shigang , Peng Jianhua , Jiang Yong 
Published: December 2022

AbstractMicroglial necroptosis exacerbates neurodegenerative diseases, central nervous system (CNS) injury, and demonstrates a proinflammatory process, but its contribution to subarachnoid hemorrhage (SAH) is poorly characterized. BCL‐2 homologous antagonist‐killer protein (Bak1), a critical regulatory molecule of endogenous apoptosis, can be involved in the pathologic process of necroptosis by regulating mitochondrial permeability. In this study, we revealed microglia undergo necroptosis after SAH in vivo and vitro. Western blot revealed that Bak1 was elevated at 24 h after SAH. Knocked down of Bak1 by adeno‐associated virus attenuates microglial necroptosis, alleviates neuroinflammation, and improves neurologic function after SAH in mice. Furthermore, oxyhemoglobin (10 μM) induced necroptosis in BV2 microglia, increasing Bak1 expression and mediating proinflammatory phenotype transformation, exacerbating oxidative stress and neuroinflammation. Abrogating BV2 Bak1 could reduce necroptosis by down‐regulating the expression of phosphorylated pseudokinase mixed lineage kinase domain‐like protein (p‐MLKL), then down‐regulating proinflammatory phenotype gene expression. RNA‐Seq showed that disrupting BV2 Bak1 down‐regulates multiple immune and inflammatory pathways and ameliorates cell injury by elevating thrombospondin 1 (THBS1) expression. In summary, we identified a critical regulatory role for Bak1 in microglial necroptosis and neuroinflammation after SAH. Bak1 is expected to be a potential target for the treatment strategy of SAH.image
Adult hippocampal neurogenesis and social behavioural deficits in the R451C Neuroligin3 mouse model of autism are reverted by the antidepressant fluoxetine
Authors: Gioia Roberta , Seri Tommaso , Diamanti Tamara , Fimmanò Stefania , Vitale Marina , Ahlenius Henrik , Kokaia Zaal , Tirone Felice , Micheli Laura , Biagioni Stefano , Lupo Giuseppe , Rinaldi Arianna , De Jaco Antonella , Cacci Emanuele 
Published: December 2022

AbstractNeuron generation persists throughout life in the hippocampus but is altered in animal models of neurological and neuropsychiatric diseases, suggesting that disease‐associated decline in cognitive and emotional hippocampal‐dependent behaviours might be functionally linked with dysregulation of postnatal neurogenesis. Depletion of the adult neural stem/progenitor cell (NSPCs) pool and neurogenic decline have been recently described in mice expressing synaptic susceptibility genes associated with autism spectrum disorder (ASDs). To gain further insight into mechanisms regulating neurogenesis in mice carrying mutations in synaptic genes related to monogenic ASDs, we used the R451C Neuroligin3 knock‐in (Nlgn3 KI) mouse, which is characterized by structural brain abnormalities, deficits in synaptic functions and reduced sociability. We show that the number of adult‐born neurons, but not the size of the NSPC pool, was reduced in the ventral dentate gyrus in knock‐in mice. Notably, this neurogenic decline was rescued by daily injecting mice with 10 mg/Kg of the antidepressant fluoxetine for 20 consecutive days. Sustained treatment also improved KI mice's sociability and increased the number of c‐Fos active adult‐born neurons, compared with vehicle‐injected KI mice. Our study uncovers neurogenesis‐mediated alterations in the brain of R451C KI mouse, showing that the R451C Nlgn3 mutation leads to lasting, albeit pharmacologically reversible, changes in the brain, affecting neuron formation in the adult hippocampus. Our results suggest that fluoxetine can ameliorate social behaviour in KI mice, at least in part, by rescuing adult hippocampal neurogenesis, which may be relevant for the pharmacological treatment of ASDs.image
Randomised controlled trial on testing an increased vertical dimension of occlusion prior to restorative treatment of tooth wear
Authors: Crins Luuk A. M. J. , Opdam Niek J. M. , Kreulen Cees M. , Bronkhorst Ewald M. , Huysmans Marie‐Charlotte D. N. J. M. , Loomans Bas A. C. 
Published: December 2022

AbstractBackgroundEvaluation of a new vertical dimension of occlusion (VDO) in complex restorative treatments is considered a necessary step prior to placement of restorations.ObjectivesThis randomised controlled trial (RCT) aimed to assess the effects of using an evaluation of a VDO increase before restorative treatment in patients with moderate‐to‐severe tooth wear, on OHRQoL, freeway space (FWS) and interventions to restorations.MethodsForty‐two patients with tooth wear were included and randomly allocated to either a test phase with a Removable Appliance (RA) or no test phase. Restorative treatment consisted of restoration of all teeth using composite restorations in an increased VDO. OHIP‐score, freeway space (FWS) and clinical acceptability of restorations were assessed at baseline and at recall appointments (1 month and 1 year). Intervention to restoration was scored in case of material chipping or when the abutment tooth had increased sensitivity that could be linked to occlusal overloading. ANCOVA analyses, Univariate Cox regression, t‐tests and descriptive analyses were performed (p < .05).ResultsClinical follow‐up after 1 year was completed for 41 patients. No significant effect of testing the VDO with a RA could be found on the OHIP‐score (p = .14). Reduction of FWS in the RA group, compared to the control group, was significantly lower at 1 year (p = .01, 95% CI −1.09 to −0.15). No effect on early interventions to restorations was found (p = .94).ConclusionThis RCT showed that a removable appliance is not indicated to functionally test the increased VDO prior to restorative treatment in patients with tooth wear.
Psoriatic arthritis of the temporomandibular joint: A systematic review
Authors: Almășan Oana , Hedeșiu Mihaela , Băciuț Mihaela , Buduru Smaranda , Dinu Cristian 
Published: December 2022

AbstractObjectivesPsoriasis is an inflammatory condition brought on by the immune system. This study aimed to perform a systematic review related to psoriatic arthritis (PsA) of the temporomandibular joint (TMJ).MethodsThe search strategy was developed by a radiologist expert with more than 20 years of experience. The search was performed without time restrictions in five electronic databases: PubMed, Web of Science, Embase, Scopus and Ovid. The search strategy was based on MeSH and Emtree terms. The methodological quality of the studies was rated using the quality assessment tools from the National Heart Lung and Blood Institute (NHLBI).ResultsTwenty‐three publications were included, 10 being case reports. One hundred‐fifty‐one patients with TMJ PsA were reported. Psoriasis evolution ranged from 1.5 years to 24 years. Clinical symptoms of TMJ involvement included: TMJ pain and sounds, limited range of jaw movements, preauricular swelling, malocclusion, headache, tinnitus, neck stiffness and altered dietary function. TMJ was evaluated by magnetic resonance imaging (six studies), computed tomography (eight articles) and by ultrasonography findings (two articles). For TMJ treatment, topical and systemic medication was reported in 11 studies. Five studies included patients needing surgical procedures for TMJ ankylosis.ConclusionsA relationship between TMD and psoriasis has been revealed. TMJ PsA has been investigated and debated, although the radiographic findings or clinical symptoms of PsA are not noticeably different from other forms of TMJ arthritis. Conservative therapy can lead to significant improvement of TMJ function.
Exosomes from hypoxia‐pretreated adipose‐derived stem cells attenuate ultraviolet light‐induced skin injury via delivery of circ‐Ash1l
Authors: Zha Jindong , Pan Yanbing , Liu Xiufeng , Zhu Hong , Liu Yanting , Zeng Weihui 
Published: December 2022

AbstractBackgroundAn increasing number of studies have reported that exosomes from adipose‐derived stem cells (ADSCs) have antioxidant and anti‐inflammatory properties. In the present study, we aimed at elucidating the potential therapeutic mechanism underlying ADSC exosomes in ultraviolet B‐light (UVB)‐induced skin injury.MethodsWe isolated the exosomes from ADSCs and hypoxia‐pretreated ADSCs. High‐throughput sequencing was applied to identify differential circRNA expression. Then, a UV‐induced murine skin injury model was constructed and the therapeutic effect of exosomes was determined using immunofluorescence and ELISA. The regulatory mechanism was demonstrated using luciferase reporter analysis and an in vitro experiment.ResultsExosomes from hypoxia‐pretreated ADSCs inhibited UVB light‐induced vascular injury by reversing ROS and inflammatory factor expression. High‐throughput sequencing showed that exosomes from hypoxia‐pretreated ADSCs (HExo) improved UV‐induced skin damage via delivery of circ‐Ash1l. Downregulation of circ‐Ash1l inhibited the therapeutic effect of HExo on UV‐induced skin damage. It was further shown that GPX4 and miR‐700‐5p were circ‐Ash1l downstream targets. MiR‐700‐5p overexpression or GPX4 downregulation inhibited the circ‐Ash1l protective effects of UV‐induced endothelial progenitor cell (EPC) damage.ConclusionThus, exosomes from hypoxia‐pretreated ADSCs attenuated UV light‐induced skin injury via circ‐Ash1l delivery and ferroptosis inhibition.
Effects of probiotics supplementation on skin photoaging and skin barrier function: A systematic review and meta‐analysis
Authors: Jwo Jhen‐Yu , Chang Ya‐Ting , Huang Yu‐Chen 
Published: December 2022

AbstractBackgroundUltraviolet (UV) irradiation is one of the major causes of skin aging. To date, there remains limited evidence on using oral probiotics for skin anti‐photoaging.ObjectivesThis systematic review and meta‐analysis aims to assess the effects of probiotics on skin photoaging.MethodsWe searched the PubMed and Embase databases for studies published until August 31, 2021, and included randomized controlled trials, murine randomized controlled experiments, and in vitro studies. Skin barrier function was compared between UV irradiated probiotics and controls.ResultsAfter given oral probiotics supplements, a significant reduction was shown in all types of measured MMPs in both murine and in vitro studies. Oral probiotics supplementation significantly reversed UV‐induced increase of epidermal thickness (standardized mean differences [SMD] = −1.76; 95% confidence interval [CI] = −3.04 to −0.49; p = .007; I2 = 81%), UV‐induced increase trans‐epidermal water loss (SMD = −1.72; 95% CI = −2.76 to −0.67; p = .001; I2 = 57%), and UV‐induced skin dehydration (SMD = 1.85; 95% CI = 1.16 to 2.55; p < .00001; I2 = 4%).ConclusionsProbiotics are effective against skin photoaging in terms of MMP pathways and reversing skin barrier function from murine randomized data. Further randomized controlled trials on humans are required to warrant these results.
Milk fatty acids as indicators of delayed commencement of luteal activity in dairy cows in early lactation
Authors: Ntallaris Theodoros , Båge Renée , Karlsson Johanna , Holtenius Kjell 
Published: December 2022

AbstractExcessive mobilization of adipose reserves due to severe negative energy balance in early lactation may be detrimental to dairy cow fertility at individual and herd level. Reproductive efficiency is one of the main factors influencing herd profitability and a strategy for early detection and management of cows with delayed resumption of cyclicity will result higher conception rate, decreased proportion of cows with extended lactation, fewer inseminations per conception and lower culling rates due to reproductive disorders. Using two groups of dairy cows (Holstein n = 37, Swedish Red breed [SRB] n = 49), we investigated potential differences between cows with different commencement of luteal activity (CLA) and the feasibility of using milk fatty acids (MFAs) as predictors of delayed CLA. Milk samples for progesterone analysis were collected twice weekly during the first six weeks in milk. The concentrations of the MFAs C14:0, C16:0, C18:0 and C18:1 cis‐9 in milk (g/100 g milk) and in milk fat (g/100 g fat) were analysed by Fourier transform infrared spectroscopy and individual MFA profiles were calculated by weeks in milk. Commencement of luteal activity was defined as the first day with milk progesterone concentrations >3 ng/ml at two successive measurements. The study population was categorized as early (n = 42) or late (n = 44) CLA, using the median value of 21 DIM as the cut‐off. Analysis of the data revealed that CLA was correlated with the proportion of some specific MFAs, where cows with delayed CLA had lower IGF‐1 (92.9 ± 7.9 vs. 114.1 ± 7.9 ng/ml; p = .05) and C14:0 levels (10.4 ± 0.2 vs. 11.5 ± 0.2 g/100 fat; p < .01) and higher C18:0 (9.6 ± 0.2 vs. 8.9 ± 0.6 g/100 fat; p < .01) and C18:1 cis‐9 levels (24.9 ± 0.4 vs. 23.5 ± 0.4 g/100 fat; p < .05). Delayed CLA (mean 34 days) was predictable for approximately 80% of cows based on C18:0 or C18:1 cis‐9 concentrations in week 2 postpartum. Overall, MFAs (C18:0 and C18:1 cis‐9) as biomarkers were better indicators than beta‐hydroxybutyrate or non‐esterified fatty acids in early detection of cows with delayed or normal CLA. The MFA concentrations in milk samples from cows in early lactation can thus be used as a non‐invasive method to identify cows at risk of delayed CLA, acting as potential biomarkers for future reproductive performance.
Growth‐regulating factors: conserved and divergent roles in plant growth and development and potential value for crop improvement
Authors: Liu Yutong , Guo Peng , Wang Jie , Xu Zheng‐Yi 
Published: December 2022

SUMMARYHigh yield and stress resistance are the major prerequisites for successful crop cultivation, and can be achieved by modifying plant architecture. Evolutionarily conserved growth‐regulating factors (GRFs) control the growth of different tissues and organs of plants. Here, we provide a systematic overview of the expression patterns of GRF genes and the structural features of GRF proteins in different plant species. Moreover, we illustrate the conserved and divergent roles of GRFs, microRNA396 (miR396), and GRF‐interacting factors (GIFs) in leaf, root, and flower development. We also describe the molecular networks involving the miR396–GRF–GIF module, and illustrate how this module coordinates with different signaling molecules and transcriptional regulators to control development of different plant species. GRFs promote leaf growth, accelerate grain filling, and increase grain size and weight. We also provide some molecular insight into how coordination between GRFs and other signaling modules enhances crop productivity; for instance, how the GRF–DELLA interaction confers yield‐enhancing dwarfism while increasing grain yield. Finally, we discuss how the GRF‐GIF chimera substantially improves plant transformation efficiency by accelerating shoot formation. Overall, we systematically review the conserved and divergent roles of GRFs and the miR396–GRF–GIF module in growth regulation, and also provide insights into how GRFs can be utilized to improve the productivity and nutrient content of crop plants.
Co-segregation analysis and functional trial in vivo of candidate genes for monogenic diabetes
Authors: Stankute Ingrida , Kazlauskiene Mintaute , Blouin Jean-Louis , Schwitzgebel Valerie M , Verkauskiene Rasa 
Published: December 2022

IntroductionThe aim of this study was to perform familial co-segregation analysis and functional trial in vivo during mixed meal tolerance test (MMTT) of novel variants in diabetes candidate genes.Research design and methodsIt is a continuation of the project “Genetic diabetes in Lithuania” with the cohort of 1209 patients with diabetes. Prior screening for autoimmune markers confirmed type 1 diabetes (T1D) diagnosis in 88.1% (n=1065) of patients, and targeted next-generation sequencing identified 3.5% (n=42) pathogenic variants in MODY genes. Subsequently, 102 patients were classified as having diabetes of unknown etiology. 12/102 were found to have novel variants in potential diabetes genes (RFX2,RREB1,SLC5A1(3 patients with variants in this gene),GCKR,MC4R,CASP10,TMPRSS6,HGFAC,DACH1,ZBED3). Co-segregation analysis and MMTT were carried out in order to study beta-cell function in subjects with specific variants.ResultsMMTT analysis showed that probands with variants inMC4R,CASP10,TMPRSS6,HGFAC, andSLC5A1(c.1415T>C) had sufficient residual beta-cell function with stimulated C-peptide (CP) >200 pmol/L. Seven individuals with variants inRFX2,RREB1,GCKR,DACH1,ZBED3andSLC5A1(c.1415T>C, and c.932A>T) presented with complete beta-cell failure. No statistical differences were found between patients with sufficient CP production and those with complete beta-cell failure when comparing age at the onset and duration of diabetes. Nineteen family members were included in co-segregation analysis; no diabetes cases were reported among them. Only in patient with the variant c.1894G>A inRFX2gene, none of the family members were affected by proband’s variant.ConclusionsFunctional beta-cell study in vivo allowed to select five most probable genes for monogenic diabetes. Familial co-segregation analysis showed that novel variant inRFX2gene could be a possible cause of diabetes. Future functional analysis in vitro is necessary to support or rule out the genetic background as a cause of diabetes.
Deciphering changes in the incidence of the Guillain-Barré syndrome during the COVID-19 pandemic: a nationwide time-series correlation study
Authors: Lee Hyunju , Heo Namwoo , Kwon Donghyok , Ha Jongmok 
Published: December 2022

BackgroundPostinfectious autoimmunity is a hallmark of Guillain-Barré syndrome (GBS), and GBS incidence closely parallels that of its immune triggers. Sociobehavioural interventions implemented during the COVID-19 pandemic have altered the infectious disease landscape.MethodsThis nationwide time-series correlation study analysed GBS incidence, sentinel surveillance and SARS-CoV-2 vaccination data from January 2017 to December 2021 in the National Health Insurance Service and Korean Disease Control and Prevention Agency databases. The incidence of GBS and sentinel gastrointestinal and respiratory infectious diseases during the pandemic (2020–2021) was estimated and compared with both prepandemic (2017–2019) and incidence predicted in a time-series forecasting model. Time-series correlation analysis was used to examine the temporal association between GBS, infectious triggers and SARS-CoV-2 vaccination.ResultsDuring the pandemic, the total crude cumulative incidence rate was 2.1 per 100 000 population, which is lower than the prepandemic incidence, especially in age groups of less than 60 years. Seasonality was briefly interrupted during the winter of 2021. The majority of respiratory and some gastrointestinal conditions had a lower-than-expected incidence during the pandemic. Compared with the prepandemic state, during the pandemic period a higher number of gastrointestinal pathogens (Escherichia coli,Campylobacterspp., Clostridium perfringens, Yersinia enterocoliticaand enteric adenovirus) had significant, moderate-to-strong positive temporal associations with GBS. The temporal association between SARS-CoV-2 infection and GBS was not significant, but SARS-CoV-2 vaccination exhibited a strong positive temporal association with GBS in 2021.ConclusionThe incidence of GBS and sentinel infectious diseases decreased to below-expected levels during the pandemic, with the former attributable to the decreased incidence of non-COVID-19 respiratory and gastrointestinal infections. The evolving incidence of autoimmune postinfectious phenomena following the pandemic needs attention.
A new ranking index to identify the work-related psychosocial factors most impacting mental health: a cross-sectional study
Authors: Daouda Oumou Salama , Chevance Astrid , Temime Laura , Légeron Patrick , Gaillard Raphaël , Saporta Gilbert , Hocine Mounia N 
Published: December 2022

ObjectivesIn modern professional life, mental health prevention and promotion have become a major challenge for decision-makers. Devising appropriate actions requires better understanding the role played by each work-related psychosocial factor (WPSF). The objective of this study was to present a relevant tool to hierarchise WPSFs that jointly takes into account theirimportance(impact on mental health) and theirprevalence(the proportion of the population exposed to WPSF).DesignA cross-sectional study was conducted in March 2018 among 3200 French workers which are representative of the French working population.SettingFrance.ParticipantsIndividuals aged 18–80 years who declared currently having a job (even a part-time job) whatever their occupation or status (employee or self-employed) were eligible. We excluded students, unemployed individuals, housewives/husbands and retired people. The mental health level was assessed using the General Health Questionnaire-28 and 44 items were gathered from theoretical models of WPSFs. We assessed two distinct multivariate methods for calculating WPSF importance: (1) weifila (weighted first last) method in a linear regression context and (2) random forests in a non-linear context. Both methods were adjusted on individual, health and job characteristics.ResultsThe WPSF rankings obtained with the two methods to calculate importance are strongly consistent with each other (correlation coefficient=0.88). We highlighted nine WPSFs that are ranked high by both methods. In particular, irrespective of the chosen method, lack of communication, lack of social and hierarchy support and personal–professional life imbalance, emotional demands at work and dissatisfaction with the compensation received came out as top-ranking WPSFs.ConclusionsA total of nine WPSFs were identified as key for decision-making. The easy-to-use tools we propose can help decision-makers identify priority WPSFs and design effective strategies to promote mental health in the workplace.