bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological traits for rapid and simple separation of native and introduced common reed (Phragmites australis)
Authors: McTavish Michael J. , Smith Tyler , Mechanda Subbaiah , Smith Sandy M. , Bourchier Robert S. 
Published: June 2023

AbstractEffective management of the introduced invasive grass common reed [Phragmites australis (Cav.) Trin. ex Steud.] requires the ability to differentiate between the introduced and native subspecies found in North America. While genetic tools are useful for discriminating between the subspecies, morphological identification is a useful complementary approach that is low to zero cost and does not require specialized equipment or technical expertise. The objective of our study was to identify the best morphological traits for rapid and simple identification of native and introduced P. australis. A suite of 22 morphological traits were measured in 21 introduced and 27 native P. australis populations identified by genetic barcoding across southern Ontario, Canada. Traits were compared between the subspecies to identify measurements that offered reliable, diagnostic separation. Overall, 21 of the 22 traits differed between the subspecies, with four offering complete separation: the retention of leaf sheaths on dead stems; a categorical assessment of stem color; the base height of the ligule, excluding the hairy fringe; and a combined measurement of leaf length and lower glume length. Additionally, round fungal spots on the stem occurred only on the native subspecies and never on the sampled introduced populations. The high degree of variation observed in traits within and between the subspecies cautions against a “common wisdom” approach to identification or automatic interpretation of intermediate traits as indicative of aberrant populations or hybridization. As an alternative, we have compiled the five best traits into a checklist of simple and reliable measurements to identify native and introduced P. australis. This guide will be most applicable for samples collected in the late summer and fall in the Great Lakes region but can also inform best practices for morphological identification in other regions as well.
Barrier Lyapunov function and adaptive backstepping-based control of a quadrotor UAV
Authors: Khadhraoui Adel , Zouaoui Amir , Saad Mohamad 
Published: June 2023

AbstractThis paper presents backstepping control and backstepping constraint control approaches for a quadrotor unmanned aerial vehicle (UAV) control system. The proposed methods are applied to a Parrot Mambo drone model to control rotational motion along the
$x$
,
$y$
, and
$z$
axes during hovering and trajectory tracking. In the backstepping control approach, each state of the system controls the previous state and is called “virtual control.” The last state is controlled by the real control input. The idea is to compute, in several steps, a control law that ensures the asymptotic stability of the system. The backstepping constraint control method, based on barrier Lyapunov functions (BLFs), is designed not only to track the desired trajectory but also to guarantee no violation of the position and angle constraints. Symmetric BLFs are introduced in the design of the controller. A nonlinear mathematical model is considered in this study. Based on Lyapunov stability theory, it can be concluded that the proposed controllers can guarantee the stability of the UAV system and the state converges asymptotically to the desired trajectory. To make the control robust, an adaptation law is applied to the backstepping control that estimates the unknown parameters and ensures their convergence to their respective values. Validation of the proposed controllers was performed by simulation on a flying UAV system.
Associations of students’ linguistic distance to the language of instruction and classroom composition with English reading and listening skills
Authors: Jaekel Nils , Ritter Markus , Jaekel Julia 
Published: June 2023

AbstractGlobally classrooms are increasingly linguistically diverse. Research often oversimplifies lived linguistic heterogeneity as binary variables: native versus non-native. Linguistic distance (LD) measures allow a fine-grained operationalization of linguistic diversity in foreign language education. This study investigated associations of cognate LDs of students’ home languages and classroom heterogeneity with English as a Foreign Language skills. Data were collected from a diverse sample of 5,130 Year 5 students in Germany. Mixed-effects linear models confirmed our hypotheses that higher individual LDs and a higher proportion of multilingual learners per classroom were both independently associated with lower English proficiency. Multilingual learners with higher cognate LDs to English and students in more linguistically heterogeneous classrooms had lower English proficiency. The results emphasize the need to assess LD in research to better differentiate between students. Foreign language classrooms seem not to address linguistic diversity adequately and need to readjust their focus to better meet multilingual learners’ needs.
Constraining Water Depth Influence on Organic Paleotemperature Proxies Using Sedimentary Archives
Authors: Varma Devika , Hättig Katrin , van der Meer Marcel T. J. , Reichart Gert‐Jan , Schouten Stefan 
Published: June 2023

AbstractThe TEX86 paleothermometer has been extensively used to reconstruct past sea water temperatures, but it remains unclear which export depths the proxy represents. Here we used a novel approach to better constrain the proxy recording depths by investigating paleotemperature proxies (TEX86, , RI−OH and RI−OH′) from two pairs of proximal (<12 km apart) cores from Chilean and Angola margins, respectively. These cores are from steep continental slopes and lower shelves, which leads to a substantial difference in water depth between them despite being closely located. Surprisingly, the deep and the shallow records at the Chilean margin show dissimilarities, in contrast to the similar records from the Angola margin, which may be due to post‐depositional alteration at the former sites. In contrast, the TEX86 records were statistically indistinguishable between the sites at both the locations, even though the GDGT [2]/[3] ratio suggests GDGTs derived from potentially different archaeal communities residing at different depths. A short‐lived difference between the TEX86 records is observed during the last glacial period at the Angola margin, possibly due to a contribution of Antarctic Intermediate Waters to the deep site. Modelling suggests that the TEX86 source signal at our core sites reaches its peak abundance at water depths shallower than 350 m. The RI−OH and RI−OH′ records show similar variability as the TEX86 records, although regional differences in their absolute temperature estimates exist. Our approach using proximal sediment cores at steep slopes appears useful to constrain the export depth of organic proxy signals for paleo‐reconstructions.
Inhibition of dorsal raphe GABAergic neurons blocks hyperalgesia during heroin withdrawal
Authors: Alvarez-Bagnarol Yocasta , García Raul , Vendruscolo Leandro F. , Morales Marisela 
Published: June 2023

AbstractOpioid withdrawal signs, such as hyperalgesia, are manifestations of opioid use disorder that may contribute to opioid seeking and taking. We have previously identified an association between dorsal raphe (DR) neurons and the expression of hyperalgesia during spontaneous heroin withdrawal. Here, we found that chemogenetic inhibition of DR neurons decreased hyperalgesia during spontaneous heroin withdrawal in male and female C57/B6 mice. By neuroanatomy, we identified three major subtypes of DR neurons expressing μ-opioid receptors (MOR) that were activated in hyperalgesia during spontaneous withdrawal, those expressing vesicular GABA transporter (VGaT), glutamate transporter 3 (VGluT3), or co-expressing VGluT3 and tryptophan hydroxylase (TPH). In contrast, we identified a small population of DR-MOR neurons expressing solely TPH, which were not activated in hyperalgesia during spontaneous withdrawal. Collectively, these findings indicate a role of the DR in hyperalgesia during spontaneous heroin withdrawal mediated, in part, by the activation of local MOR-GABAergic, MOR-glutamatergic and MOR-co-releasing glutamatergic-serotonergic neurons. We found that specific chemogenetic inhibition of DR-VGaT neurons blocked hyperalgesia during spontaneous heroin withdrawal in male and female mice. Collectively, these findings indicate that DR-GABAergic neurons play a role in the expression of hyperalgesia during spontaneous heroin withdrawal.
Distinct bacterial population dynamics and disease dissemination after biofilm dispersal and disassembly
Authors: Ma Yeping , Deng Yanlin , Hua Haojun , Khoo Bee Luan , Chua Song Lin 
Published: June 2023

Abstract
Microbial communities that form surface-attached biofilms must release and disperse their constituent cells into the environment to colonize fresh sites for continued survival of their species. For pathogens, biofilm dispersal is crucial for microbial transmission from environmental reservoirs to hosts, cross-host transmission, and dissemination of infections across tissues within the host. However, research on biofilm dispersal and its consequences in colonization of fresh sites remain poorly understood. Bacterial cells can depart from biofilms via stimuli-induced dispersal or disassembly due to direct degradation of the biofilm matrix, but the complex heterogeneity of bacterial populations released from biofilms rendered their study difficult. Using a novel 3D-bacterial “biofilm-dispersal-then-recolonization” (BDR) microfluidic model, we demonstrated that Pseudomonas aeruginosa biofilms undergo distinct spatiotemporal dynamics during chemical-induced dispersal (CID) and enzymatic disassembly (EDA), with contrasting consequences in recolonization and disease dissemination. Active CID required bacteria to employ bdlA dispersal gene and flagella to depart from biofilms as single cells at consistent velocities but could not recolonize fresh surfaces. This prevented the disseminated bacteria cells from infecting lung spheroids and Caenorhabditis elegans in on-chip coculture experiments. In contrast, EDA by degradation of a major biofilm exopolysaccharide (Psl) released immotile aggregates at high initial velocities, enabling the bacteria to recolonize fresh surfaces and cause infections in the hosts efficiently. Hence, biofilm dispersal is more complex than previously thought, where bacterial populations adopting distinct behavior after biofilm departure may be the key to survival of bacterial species and dissemination of diseases.
METTL3-dependent m6A methylation facilitates uterine receptivity and female fertility via balancing estrogen and progesterone signaling
Authors: Wan Shuo , Sun Yadong , Zong Jinbao , Meng Wanqing , Yan Jiacong , Chen Kexin , Wang Sanfeng , Guo Daji , Xiao Zhiqiang , Zhou Qinghua , Yin Zhinan , Yang Meixiang 
Published: June 2023

AbstractInfertility is a worldwide reproductive health problem and there are still many unknown etiologies of infertility. In recent years, increasing evidence emerged and confirmed that epigenetic regulation played a leading role in reproduction. However, the function of m6A modification in infertility remains unknown. Here we report that METTL3-dependent m6A methylation plays an essential role in female fertility via balancing the estrogen and progesterone signaling. Analysis of GEO datasets reveal a significant downregulation of METTL3 expression in the uterus of infertile women with endometriosis or recurrent implantation failure. Conditional deletion of Mettl3 in female reproductive tract by using a Pgr-Cre driver results in infertility due to compromised uterine endometrium receptivity and decidualization. m6A-seq analysis of the uterus identifies the 3’UTR of several estrogen-responsive genes with METTL3-dependent m6A modification, like Elf3 and Celsr2, whose mRNAs become more stable upon Mettl3 depletion. However, the decreased expression levels of PR and its target genes, including Myc, in the endometrium of Mettl3 cKO mice indicate a deficiency in progesterone responsiveness. In vitro, Myc overexpression could partially compensate for uterine decidualization failure caused by Mettl3 deficiency. Collectively, this study reveals the role of METTL3-dependent m6A modification in female fertility and provides insight into the pathology of infertility and pregnancy management.
A novel N6-Deoxyadenine methyltransferase METL-9 modulates C. elegans immunity via dichotomous mechanisms
Authors: Ma Chengchuan , Xue Tingling , Peng Qi , Zhang Jie , Guan Jialiang , Ding Wanqiu , Li Yi , Xia Peixue , Zhou Liankui , Zhao Tianyu , Wang Sheng , Quan Li , Li Chuan-Yun , Liu Ying 
Published: June 2023

AbstractN6-Methyldeoxyadenine (6mA) has been rediscovered as a DNA modification with potential biological function in metazoans. However, the physiological function and regulatory mechanisms regarding the establishment, maintenance and removal of 6mA in eukaryotes are still poorly understood. Here we show that genomic 6mA levels change in response to pathogenic infection in Caenorhabditis elegans (C. elegans). We further identify METL-9 as the methyltransferase that catalyzes DNA 6mA modifications upon pathogen infection. Deficiency of METL-9 impairs the induction of innate immune response genes and renders the animals more susceptible to pathogen infection. Interestingly, METL-9 functions through both 6mA-dependent and -independent mechanisms to transcriptionally regulate innate immunity. Our findings reveal that 6mA is a functional DNA modification in immunomodulation in C. elegans.
Nanowire-based smart windows combining electro- and thermochromics for dynamic regulation of solar radiation
Authors: Sheng Si-Zhe , Wang Jin-Long , Zhao Bin , He Zhen , Feng Xue-Fei , Shang Qi-Guo , Chen Cheng , Pei Gang , Zhou Jun , Liu Jian-Wei , Yu Shu-Hong 
Published: June 2023

AbstractSmart window is an attractive option for efficient heat management to minimize energy consumption and improve indoor living comfort owing to their optical properties of adjusting sunlight. To effectively improve the sunlight modulation and heat management capability of smart windows, here, we propose a co-assembly strategy to fabricate the electrochromic and thermochromic smart windows with tunable components and ordered structures for the dynamic regulation of solar radiation. Firstly, to enhance both illumination and cooling efficiency in electrochromic windows, the aspect ratio and mixed type of Au nanorods are tuned to selectively absorb the near-infrared wavelength range of 760 to 1360 nm. Furthermore, when assembled with electrochromic W18O49 nanowires in the colored state, the Au nanorods exhibit a synergistic effect, resulting in a 90% reduction of near-infrared light and a corresponding 5 °C cooling effect under 1-sun irradiation. Secondly, to extend the fixed response temperature value to a wider range of 30–50 °C in thermochromic windows, the doping amount and mixed type of W-VO2 nanowires are carefully regulated. Last but not the least, the ordered assembly structure of the nanowires can greatly reduce the level of haze and enhance visibility in the windows.
The curious case of the structural phase transition in SnSe insights from neutron total scattering
Authors: Jiang Bo , Neu Jennifer , Olds Daniel , Kimber Simon A. J. , Page Katharine , Siegrist Theo 
Published: June 2023

AbstractAt elevated temperatures SnSe is reported to undergo a structural transition from the low symmetry orthorhombic GeS-type to a higher symmetry orthorhombic TlI-type. Although increasing symmetry should likewise increase lattice thermal conductivity, many experiments on single crystals and polycrystalline materials indicate that this is not the case. Here we present temperature dependent analysis of time-of-flight (TOF) neutron total scattering data in combination with theoretical modeling to probe the local to long-range evolution of the structure. We report that while SnSe is well characterized on average within the high symmetry space group above the transition, over length scales of a few unit cells SnSe remains better characterized in the low symmetry GeS-type space group. Our finding from robust modeling provides further insight into the curious case of a dynamic order-disorder phase transition in SnSe, a model consistent with the soft-phonon picture of the high thermoelectric power above the phase transition.
Structural mapping of Nav1.7 antagonists
Authors: Wu Qiurong , Huang Jian , Fan Xiao , Wang Kan , Jin Xueqin , Huang Gaoxingyu , Li Jiaao , Pan Xiaojing , Yan Nieng 
Published: June 2023

AbstractVoltage-gated sodium (Nav) channels are targeted by a number of widely used and investigational drugs for the treatment of epilepsy, arrhythmia, pain, and other disorders. Despite recent advances in structural elucidation of Nav channels, the binding mode of most Nav-targeting drugs remains unknown. Here we report high-resolution cryo-EM structures of human Nav1.7 treated with drugs and lead compounds with representative chemical backbones at resolutions of 2.6-3.2 Å. A binding site beneath the intracellular gate (site BIG) accommodates carbamazepine, bupivacaine, and lacosamide. Unexpectedly, a second molecule of lacosamide plugs into the selectivity filter from the central cavity. Fenestrations are popular sites for various state-dependent drugs. We show that vinpocetine, a synthetic derivative of a vinca alkaloid, and hardwickiic acid, a natural product with antinociceptive effect, bind to the III-IV fenestration, while vixotrigine, an analgesic candidate, penetrates the IV-I fenestration of the pore domain. Our results permit building a 3D structural map for known drug-binding sites on Nav channels summarized from the present and previous structures.
In silico screening for As/Se-free ovonic threshold switching materials
Authors: Clima Sergiu , Matsubayashi Daisuke , Ravsher Taras , Garbin Daniele , Delhougne Romain , Kar Gouri Sankar , Pourtois Geoffrey 
Published: June 2023

AbstractRestricted use of hazardous environmental chemicals is one important challenge that the semiconductor industry needs to face to improve its sustainability. Ovonic threshold switching (OTS) ternary compound materials used in memory selector devices contain As and Se. Engineering these elements out of these materials requires significant research effort. To facilitate this process, we performed systematic material screening for As/Se-free ternary materials, based on ab-initio simulations. To limit the large amount of possible chemical compositions to fewer promising candidates, we used physics-based material parameter filters like material stability, electronic properties, or change in polarizability. The OTS gauge concept is introduced as a computed parameter to estimate the probability of a material to show an OTS behavior. As a result, we identified 35 As/Se-free ternary alloy compositions for stand-alone OTS memory applications, as well as 12 compositions for RRAM selector applications. This work aims seeding the development of As/Se-free OTS materials.
Janus porous polylactic acid membranes with versatile metal–phenolic interface for biomimetic periodontal bone regeneration
Authors: Zhang Yaping , Chen Yi , Ding Tian , Zhang Yandi , Yang Daiwei , Zhao Yajun , Liu Jin , Ma Baojin , Bianco Alberto , Ge Shaohua , Li Jianhua 
Published: June 2023

AbstractConventional treatment to periodontal and many other bone defects requires the use of barrier membranes to guided tissue regeneration (GTR) and guided bone regeneration (GBR). However, current barrier membranes normally lack of the ability to actively regulate the bone repairing process. Herein, we proposed a biomimetic bone tissue engineering strategy enabled by a new type of Janus porous polylactic acid membrane (PLAM), which was fabricated by combining unidirectional evaporation-induced pore formation with subsequent self-assembly of a bioactive metal–phenolic network (MPN) nanointerface. The prepared PLAM-MPN simultaneously possesses barrier function on the dense side and bone-forming function on the porous side. In vitro, the presence of MPN nanointerface potently alleviated the proinflammatory polarization of mice bone marrow-derived macrophages (BMDMs), induced angiogenesis of human umbilical vein endothelial cells (HUVECs), and enhanced the attachment, migration and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). The implantation of PLAM-MPN into rat periodontal bone defects remarkably enhanced bone regeneration. This bioactive MPN nanointerface within a Janus porous membrane possesses versatile capacities to regulate cell physiology favoring bone regeneration, demonstrating great potential as GTR and GBR membranes for clinical applications.
Prospection deficits in patients with first-episode schizophrenia: a cross-sectional comparative study
Authors: Fung Antoinette C. O. , Zhang Rui-ting , Yip Stanley S. L. , Poon Grace K. S. , Cheng Chi-Wai , Yang Tian-xiao , Lui Simon S. Y. , Chan Raymond C. K. 
Published: June 2023

AbstractProspection refers to the ability to simulate and pre-experience future events. Schizophrenia patients have difficulty in anticipating pleasure in future events, but previous studies examined prospection deficits in chronic schizophrenia patients. This study aimed to investigate prospection deficits in first-episode schizophrenia patients. Thirty first-episode schizophrenia patients and 31 healthy controls completed the Affective Prospection Task, which utilized pictorial cues to involve positive, neutral and negative prospection. Participants’ ratings regarding the phenomenal characteristics of their prospected events were collected, and their prospected narratives were coded using a valid scoring manual. We also assessed intelligence, working memory and logical memory. The results showed, in all participants, valence of the cues significantly influenced participants’ sense of pre-experience, temporal distance, emotion experience, vividness and participation of the prospected events, as well as the richness of sensory details. The two groups did not differ in self-report phenomenal characteristics of their prospected events. For coded characteristics, schizophrenia patients’ prospected narratives were less rich in thought/emotion than controls, even after controlling for intelligence and memory deficits. We extended empirical evidence for prospection deficits from chronic schizophrenia samples to first-episode schizophrenia patients.
An all-in-one nanoprinting approach for the synthesis of a nanofilm library for unclonable anti-counterfeiting applications
Authors: Zhang Junfang , Liu Yuxin , Njel Christian , Ronneberger Sebastian , Tarakina Nadezda V. , Loeffler Felix F. 
Published: June 2023

AbstractIn addition to causing trillion-dollar economic losses every year, counterfeiting threatens human health, social equity and national security. Current materials for anti-counterfeiting labelling typically contain toxic inorganic quantum dots and the techniques to produce unclonable patterns require tedious fabrication or complex readout methods. Here we present a nanoprinting-assisted flash synthesis approach that generates fluorescent nanofilms with physical unclonable function micropatterns in milliseconds. This all-in-one approach yields quenching-resistant carbon dots in solid films, directly from simple monosaccharides. Moreover, we establish a nanofilm library comprising 1,920 experiments, offering conditions for various optical properties and microstructures. We produce 100 individual physical unclonable function patterns exhibiting near-ideal bit uniformity (0.492 ± 0.018), high uniqueness (0.498 ± 0.021) and excellent reliability (>93%). These unclonable patterns can be quickly and independently read out by fluorescence and topography scanning, greatly improving their security. An open-source deep-learning model guarantees precise authentication, even if patterns are challenged with different resolutions or devices.