bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cingulate cGMP-dependent protein kinase I facilitates chronic pain and pain-related anxiety and depression
Authors: Wang Tao-Zhi , Wang Fei , Tian Zhi-Cheng , Li Zhen-Zhen , Liu Wan-Neng , Ding Hui , Xie Ting-Ting , Cao Zi-Xuan , Li Hai-Tao , Sun Zhi-Chuan , Xie Rou-Gang , Wu Sheng-Xi , Pan Zhen-Xiang , Luo Ceng 
Published: June 2023

Abstract
Patients with chronic pain often experience exaggerated pain response and aversive emotion, such as anxiety and depression. Central plasticity in the anterior cingulate cortex (ACC) is assumed to be a critical interface for pain perception and emotion, which has been reported to involve activation of NMDA receptors. Numerous studies have documented the key significance of cGMP-dependent protein kinase I (PKG-I) as a crucial downstream target for the NMDA receptor–NO–cGMP signaling cascade in regulating neuronal plasticity and pain hypersensitivity in specific regions of pain pathway, ie, dorsal root ganglion or spinal dorsal horn. Despite this, whether and how PKG-I in the ACC contributes to cingulate plasticity and comorbidity of chronic pain and aversive emotion has remained elusive. Here, we uncovered a crucial role of cingulate PKG-I in chronic pain and comorbid anxiety and depression. Chronic pain caused by tissue inflammation or nerve injury led to upregulation of PKG-I expression at both mRNA and protein levels in the ACC. Knockdown of ACC-PKG-I relieved pain hypersensitivity as well as pain-associated anxiety and depression. Further mechanistic analysis revealed that PKG-I might act to phosphorylate TRPC3 and TRPC6, leading to enhancement of calcium influx and neuronal hyperexcitability as well as synaptic potentiation, which results in the exaggerated pain response and comorbid anxiety and depression. We believe this study sheds new light on the functional capability of ACC-PKG-I in modulating chronic pain as well as pain-associated anxiety and depression. Hence, cingulate PKG-I may represent a new therapeutic target against chronic pain and pain-related anxiety and depression.
Economic analysis of patient-related effects of an interdisciplinary pain self-management program
Authors: Chowdhury Anonnya Rizwana , Schofield Deborah , Shrestha Rupendra , Nicholas Michael 
Published: June 2023

Abstract
Active Day Patient Treatment (ADAPT) is a well-established 3 week intensive cognitive-behavioural, interdisciplinary pain management program for patients with disabling chronic pain. The aim of this analysis was to conduct an economic analysis of patient-related effects of ADAPT using hospital administrative data, specifically, to compare the costs and health outcomes for patients 1 month after participating in the program, with the preprogram period when they were receiving standard care. This retrospective cohort study included 230 patients who completed ADAPT (including follow-ups) between 2014 and 17 at the Pain Management and Research Centre at the Royal North Shore Hospital in Sydney, Australia. Data on pain-related healthcare utilization and costs before and after the program were assessed. Primary outcome measures were labour force participation for patients' average weekly earnings and cost per clinically meaningful change in Pain Self-efficacy Questionnaire, Brief Pain Inventory (BPI) Severity, and BPI interference scores (n = 224). We estimated patients, on average, earned $59 more each week at 1 month follow-up compared with baseline. The cost per clinically meaningful change in pain severity and interference score based on the BPI severity and BPI interference were AU$9452.32 (95% CI: $7031.76-$12,930.40) and AU$3446.62 (95% CI: $2851.67-$4126.46), respectively. The cost per point improvement and per clinically meaningful change in the Pain Self-efficacy Questionnaire were $483 (95% CI: $411.289-$568.606) and $3381.02, respectively. Our analysis showed a better health outcome, reduced healthcare services' cost, and reduced number of medications taken 1 month after participating in ADAPT.
PRESTO: Promoting Engagement for the Safe Tapering of Opioids
Authors: Bricker Dean A. , Crawford Timothy N. , Castle Angie , Anderson Maria , James Autumn M. , Hershberger Paul J. 
Published: June 2023

Abstract
Twenty-four percent of all U.S. opioid overdose deaths involve a prescription opioid. Changing prescribing practices is considered a key step in reducing opioid overdoses. Primary care providers (PCPs) commonly lack the patient engagement skills needed to address patient resistance to taper or end opioid prescriptions. We developed and evaluated a protocol aimed at improving PCP opioid-prescribing patterns and modeled on the evidence-based Screening, Brief Intervention, and Referral to Treatment (SBIRT) approach. We conducted a time series trial comparing provider opioid prescribing 8 months before and 8 months after training with the PRomoting Engagement for Safe Tapering of Opioids (PRESTO) protocol. The 148 Ohio PCPs who completed PRESTO training gained confidence in their ability to engage their patients on the topics of opioid overdose risk and potential opioid tapering. Promoting Engagement for Safe Tapering of Opioids participants had decreased opioid-prescribing over time, but this was not significantly different from Ohio PCPs who had not received PRESTO training. Participants completing PRESTO training had small, but significant increased buprenorphine prescribing over time compared with Ohio PCPs who had not received PRESTO training. The PRESTO approach and opioid risk pyramid warrant further study and validation.
Wilson disease complicated by Crohn disease: A case report and literature review
Authors: Chen Minmin , Li Chenyan , Peng Shiqiao , Liu Mengyuan , Li Yiling , Sun Mingjun , Sun Xuren 
Published: June 2023

Rational:
Wilson disease (WD), also known as hepatolenticular degeneration, is an autosomal-recessive hereditary disease with abnormal copper metabolism. Crohn disease (CD) is a chronic inflammatory gastrointestinal disease, which belongs to inflammatory bowel disease, all segments of the gastrointestinal tract can be affected, especially the terminal ileum and colon, accompanied by extraintestinal manifestations and related immune disorders. WD complicated by ulcerative colitis has been reported before, but WD complicated by CD has not been reported so far.

Patient concerns and diagnosis:
We presented the first report of a young patient with WD complicated by CD, who was admitted to the hospital because of repeated low fever, elevated C-reactive protein for 3 years, and anal fistula for 6 months.

Interventions and outcomes:
In this complicated disease, Ustekinumab is safe and effective.

Lessons:
We conclude that copper metabolism and oxidative stress play important roles in WD and CD.
The effect of music therapy on delirium in patients receiving mechanical ventilatory support in the Intensive Care Unit: A protocol for systematic review and meta-analysis
Authors: Zhou Changyan , Ma Hong , Qi Xiaoxue , Xu Chunru , Liang Zina 
Published: June 2023

Background:
Delirium is a very common sick syndrome in patients receiving mechanical ventilatory support in the Intensive Care Unit (ICU). Music therapy is a promising non-pharmacological intervention. However, its effect on the duration, incidence and severity of delirium is unknown. So we will perform a systematic review and meta-analysis to evaluate the effect of music therapy on delirium in patients receiving mechanical ventilatory support in the ICU.

Methods:
This systematic review was registered in the PROSPERO. We will follow the Preferred Reporting Items for Systematic Reviews and Meta-analysis Protocol to accomplish the systematic review protocol. Searches of PubMed, EMbase, the Cochrane library, CBM, CNKI and Wanfang databases will be conducted through computer to collect randomized controlled trials (RCTs) on the effects of music therapy on delirium in patients receiving mechanical ventilatory support in the ICU. The search time will be all from database establishment to April 2023. Two evaluators will independently screen the literature, extract information and evaluate the risk of bias of included studies, then data analysis will be performed using Stata 14.0 software.

Results:
The results of this systematic review and meta-analysis will be publicly available and published in a peer-reviewed journal.

Conclusion:
This study will provide evidence-based medical evidence for music therapy to control delirium in patients receiving mechanical ventilatory support in the ICU.
Effects of folic acid supplementation on chronic atrophic gastritis based on MTHFR C677T polymorphism
Authors: Kong Siya , Zhang Guoxin , Yang Zhen , Kong Zihao , Ye Feng 
Published: June 2023

Background:
It has been shown the methylenetetrahydrofolate reductase (MTHFR) 677TT (rs 1801133) genotype predicts histopathological alterations in the incisura of patients with chronic atrophic gastritis (CAG). MTHFR is a crucial enzyme in fatty acid (FA) metabolism. This study aimed to evaluate the influence of FA supplementation in CAG patients without Helicobacter pylori infection and the MTHFR C677T (rs 1801133) genotype as a potential CAG predictor.

Methods:
A total of 96 CAG patients, aged 21 to 72 years old, were enrolled in this study. After 6 months of treatment, histopathological outcomes were compared among patients treated with weifuchun (WFC) (1.44 g 3 times per os per day), those treated with WFC and FA (5 mg once daily), and those treated with WFC, FA, and vitamin B12 (VB12) (0.5 mg 3 times per day) based on the Operative Link on Gastritis/Intestinal Metaplasia assessment staging systems.

Results:
Atrophic lesions in patients treated with WFC and FA improved more than in patients treated only with WFC therapy (78.1% vs 53.3%, P = .04). Atrophic or intestinal metaplasia (IM) lesions in the incisura of patients with the TT genotype were better than those in patients with the CC/CT genotype (P = .02).

Conclusion:
The treatment of CAG patients with 5 mg of FA supplements daily for 6 months improved their gastric atrophy status, especially for the Operative Link on Gastritis/Intestinal Metaplasia assessment stages I/II. Moreover, our study is the first to reveal that patients with the MTHFR 677TT genotype require more timely and effective FA treatment than those with the CC/CT genotype.
Prevalence of type 2 diabetes mellitus and related factors among the general adult population in Saudi Arabia between 2016–2022: A systematic review and meta-analysis of the cross-sectional studies
Authors: Alwadeai Khalid S. , Alhammad Saad A. 
Published: June 2023

Background:
Health officials need current knowledge of type 2 diabetes mellitus (T2DM) and related risk factors to prioritize prevention and control measures due to lifestyle changes, population structure, and the high incidence of diabetes in Saudi Arabia. The present systematic review aims to estimate the current pooled prevalence of T2DM and related risk factors among the general adult population in Saudi Arabia between 2016 and 2022.

Methods:
PubMed, Web of Science, and Google Scholar databases were searched for cross-sectional studies that reported T2DM among adults in Saudi Arabia and were published between December 31, 2016, and December 31, 2022. The PRISMA guidelines and the AXIS tool were employed to report and evaluate study quality and bias risk.

Results:
The meta-analysis with a fixed effect model included 10 studies comprising 8457 general men and women adults aged 18 or older. The prevalence of T2DM was 28% (95% CI = 27–28, P < .001), and risks of T2DM in persons over 40 were nearly twice as high (OR = 1.74, 95% CI = 1.34–2.27) than in age under 40 among general adult population in Saudi Arabia between 2016–2022. This difference was statistically significant (P < .0001).

Conclusion:
The evidence from this review provided alarming and highlighted points about the prevalence of T2DM between 2016 and 2022, but there was high heterogeneity between the studies. Individuals aged 40 or older had a high risk of T2DM among the general adult population in Saudi Arabia.
Cerebellar infarction caused by vertebral artery dissection: A case report
Authors: Zhao Jing , Luo Bin , Yao Xinlu , Zhang Xiaoyun , He Daquan , Cai Lina , Xu Yahui , Li Qin , Wan Zhirong 
Published: June 2023

Rationale:
Vertebral artery dissection is an important cause of posterior circulation ischemic stroke in young and middle-aged people. We reported a young man with cerebellar infarction caused by dissection of the right vertebral artery.

Patient concerns:
A 34-year-old man presented with intermittent dizziness, blurred vision, nausea, and transient tinnitus 10 days before admission. All these symptoms were gradually aggravated and followed by vomiting and unfavorable movement of the right limbs. All these symptoms gradually aggravated.

Diagnosis:
Neurological examination on admission showed ataxia of the right limbs. Magnetic resonance imaging of the head revealed a right cerebellar infarction. High-resolution vessel wall magnetic resonance imaging showed dissection of the right vertebral artery. Whole-brain CT digital subtraction angiography revealed occlusion of the third segment (V3) of the right vertebral artery. This supports the diagnosis of vertebral artery dissection.

Interventions:
The patient received anticoagulant treatment with warfarin.

Outcomes:
After 2 weeks of treatment, the patient showed remarkably alleviated dizziness and unfavorable movement of the right limbs. After 3 months of treatment, the modified Rankin Scale score was 0. MRI of the head revealed that the original right cerebellar focus was softened, and there were no newly formed infarct foci.

Lessons:
When young and middle-aged patients without atherosclerotic risk factors encounter sudden dizziness, tinnitus, and unfavorable limb movement, vertebral artery dissection may be considered. Careful inquiry into the medical history may help make a final diagnosis. Further high-resolution vessel wall magnetic resonance imaging is an effective means to find arterial dissection. Early diagnosis and treatment for vertebral artery dissection has a favorable prognosis.
Insights into adeno-associated virus-based ocular gene therapy: A bibliometric and visual analysis
Authors: Chen Xi , Yu Yang , Nie Huiling , Qin Xun , Bai Wen , Ren Junsong , Yao Jin , Li Juxue , Jiang Qin 
Published: June 2023

Background:
Adeno-associated virus (AAV) plays a vital role in ocular gene therapy and has been widely studied since 1996. This study summarizes and explores the publication outputs and future research trends of AAV-based ocular gene therapy.

Methods:
Publications and data about AAV-based ocular gene therapy were downloaded from the Web of Science Core Collection or ClinicalTrials.gov database. The publications and data were analyzed by Microsoft Excel, CiteSpace, VOS viewer, and a free online platform (http://bibliometric.com).

Results:
Totally 832 publications from the Web of Science Core Collection relevant to AAV-based ocular gene therapy were published from 1996 to 2022. These publications were contributed by research institutes from 42 countries or regions. The US contributed the most publications among these countries or regions, notably the University of Florida. Hauswirth WW was the most productive author. “Efficacy” and “safety” are the main focus areas for future research according to the references and keywords analysis. Eighty clinical trials examined AAV-based ocular gene therapy were registered on ClinicalTrials.Gov. Institutes from the US and European did the dominant number or the large proportion of the trials.

Conclusions:
The research focus of the AAV-based ocular gene therapy has transitioned from the study in biological theory to clinical trialing. The AAV-based gene therapy is not limited to inherited retinal diseases but various ocular diseases.
Current State of Dor Yeshorim, Expanded Carrier, and Newborn Screening
Authors: Anderson Sharon 
Published: June 2023

Abstract
Availability and accessibility of preconception and prenatal genetic carrier and newborn biochemical and genetic screening have grown exponentially over the past 2 decades and as such, it is challenging for clinicians to keep pace. Although genetic counseling or genetic consultation should be offered to all expectant and new parents for prenatal screening decisions and positive results, benefits and limitations of these tests and results must be known and familiar to perinatal and pediatric clinicians. A brief historical overview of Dor Yeshorim, preconception and prenatal expanded carrier, and newborn screening is presented, followed by discussion about the conditions screened and considerations surrounding the benefits and limitations of these tests in the practice setting.
Exploring Clinical Nurse Specialist Impact in Primary Health Care: Exemplars From a Student’s Clinical Practice
Authors: Bostelman Chelsea , Passwater Chelsea , Urton Michael 
Published: June 2023

Purpose/Objectives
Primary healthcare (PHC) is a clinical frontier full of opportunities for clinical nurse specialists (CNSs) to improve population outcomes, facilitate smooth care transitions, and overcome challenges through a unique lens. Clinical nurse specialist practice in primary care is exceedingly rare, and there is a paucity of literature on the topic. This article provides exemplary projects implemented by a CNS student in a primary care clinic.

Description of the Project/Program
Primary healthcare has been described as the “front door” of the health system. It has increasingly relied on nursing to deliver health services, yet PHC and nursing practice in this setting remain poorly defined. Clinical nurse specialists are well-positioned to define these concepts, standardize processes used to deliver services, and impact patient outcomes in PHC. The CNS student successfully supported a primary care clinic in these activities.

Outcome
Reviewing the impact of the CNS student’s experience offers a better understanding of CNS practice in PHC.

Conclusion
Gaps exist in the literature regarding best practices and care delivery in PHC. Clinical nurse specialists are educationally prepared to address these gaps and improve patient outcomes at the health system’s “front door.” Utilizing a CNS’s unique skill set achieves cost-effective and efficient healthcare delivery in a new way that bolsters the strategy of utilizing nurse practitioners to meet the challenge of provider shortages.
Recurrent Deeply Invasive Basal Cell Carcinoma
Authors: Milvydaitė Greta , Ivanauskas Andrius , Keizeris Tadas , Ryškienė Silvija , Rokicki Jan Pavel , Janužis Gintaras , Razukevičius Dainius 
Published: June 2023

Introduction:
Basal cell carcinoma (BCC) is the most frequent skin malignancy worldwide. The majority of BCCs grow slowly and have a low metastatic potential. However, they can be destructive to surrounding tissues since they are locally invasive.

Methods:
This case report was conducted on a 78-year-old woman complaining of a solid lump on the left side of the neck and nonhealing lesion. Three years earlier, she had BCC on the same site. Clinical and radiographic examinations were made. The biopsy specimens revealed that it is a recurrent BCC. In operating room, during blunt tissue dissection, the arterial wall was damaged. Tumor was overgrown left internal carotid artery near the bifurcation. Infiltrated part of arteria wall was resected, and a synthetic arterial prosthesis was placed.

Results:
Follow-up after 4 months showed that the wound was healing well. No complications regarding cardiovascular and other organ systems were seen.
The Histone Methyltransferase Setdb2 Modulates TIMP-MMP Activity During Abdominal Aortic Aneurysm Development
Authors: Davis Frank M. , Melvin William J. , Mangum Kevin , Tsoi Lam C. , Joshi Amrita D. , Cai Qing , Henke Peter K. , Gudjonsson Johann E. , Gallagher Katherine A. 
Published: June 2023

Objective:To determine macrophage-specific alterations in epigenetic enzyme function contributing to development of abdominal aortic aneurysms (AAAs).Summary Background Data:AAA are a life-threatening disease, characterized by pathological vascular remodeling driven by an imbalance of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). Identifying mechanisms regulating macrophage-mediated extracellular matrix degradation is of critical importance to developing novel therapies.Methods:The role of SETDB2 in AAA formation was examined in human aortic tissue samples by single-cell RNA sequencing and in a myeloid-specific SETDB2 deficient murine model induced by challenging mice with a combination of a high-fat diet and angiotensin II.Results:Single-cell RNA sequencing of human AAA tissues, identified SETDB2 was upregulated in aortic monocyte/macrophages and murine AAA models compared to controls. Mechanistically, interferon-β regulatesSetdb2expression via JAK/STAT signaling which trimethylates histone 3 lysine 9 (H3K9) on the TIMP1-3 gene promoters thereby suppressingTimp1-3transcription and leading to unregulated MMP activity. Macrophage-specific knockout of SETDB2 (Setdb2f/fLyz2Cre+) protected mice from AAA formation with suppression of vascular inflammation, macrophage infiltration, and elastin fragmentation. Genetic depletion of SETDB2 prevented AAA development due to removal of the repressive H3K9-trimethylation mark on theTimp1-3gene promoter resulting in increased TIMP expression, decreased protease activity, and preserved aortic architecture. Lastly, inhibition of the JAK/STAT pathway with an FDA approved inhibitor, Tofacitinib, limitedSetdb2expression in aortic macrophages.Conclusions:These findings identify SETDB2 as a critical regulator of macrophage-mediated protease activity in AAAs and identify SETDB2 as a mechanistic target for the management of AAAs.
Risk Factors for De Novo Overactive Bladder After Midurethral Sling
Authors: Serna-Gallegos Tasha , Dutta Sonia , Crowder Carly , Wadensweiler Paul , Whitcomb Emily L. , Guaderrama Noelani M. 
Published: June 2023

Importance
The low incidence of de novo overactive bladder (OAB) symptoms after a midurethral sling (MUS) procedure better informs preoperative counseling.

Objective
The study aimed to measure the incidence and risk factors for de novo OAB after MUS.

Study Design
This was a retrospective cohort study of de novo OAB symptoms in patients who underwent MUS surgery in a health maintenance organization between January 1, 2008, and September 30, 2016. Patients were identified using Current Procedural Terminology codes for MUS and International Classification of Diseases, Tenth Revision codes for urinary urgency, frequency, nocturia, OAB, and urgency urinary incontinence (UUI). The cohort of patients was identified by the absence of these International Classification of Diseases, Tenth Revision codes 12 months preoperatively and the presence of these codes within 6 months after surgery. This cohort was used to calculate the rate of de novo OAB after MUS surgery. Clinical and demographic factors were abstracted. Statistical analysis was performed using descriptive, χ
2, simple logistic, and multiple logistic regression.

Results
During the study period, 13,893 patients underwent MUS surgery and 6,634 met the inclusion criteria. The mean age was 56.9 years, mean parity was 2.76, and mean body mass index was 28.9 (calculated as weight in kilograms divided by height in meters squared). Of these, 410 (6.1%) developed de novo OAB within 12 months. The most common symptoms were urgency (65.4%), UUI (42.2%), and frequency (19.8%). On multivariable regression modeling, de novo urgency and UUI were not associated with concurrent surgery (P < 0.05). Increasing age and body mass index were associated with an increased risk of nocturia (P < 0.05).

Conclusions
The incidence of de novo OAB after MUS surgery was 6.1%. This aligns with current literature and critically informs preoperative counseling for MUS surgery.