bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intrinsic High-Order Ionic Superlattices in Two-Dimensional Oxide Perovskites
Authors: Cho Kyungjune , Yim Haena , Park Gahui , Yang Jiwoo , Yoo So Yeon , Nam Jongwoo , Song Minwoo , Kwon Deok-Hwang , Kang Keehoon , Lee Takhee , Choi Ji-Won , Chung Seungjun 
Published: February 2023

Abstract
A high-order superlattice is desirable in emerging classes of semiconductors because it allows functionalities beyond the reach of existing materials. Despite their beneficial features, certain shortcomings, such as the poor interface between heterostructures and laborious time-consuming fabrication for stacking high-ordered layers, still need to be addressed. Here, we present intrinsic high-order ionic layered superlattices in two-dimensional layered Dion-Jacobson phase oxide perovskites. The atomically clean interfaces between neighbouring layers ensure unique quantum mechanical properties derived from the quantum wells of ionic layered superlattices. The resultant anomalous charging behaviour offers tenable conductivity enhancements and realises artificial synaptic devices modulated by both optical and electrical stimuli. We also demonstrate the high tunability of the ionic composition and number of layers of the two-dimensional layered oxide perovskites, resulting in tailored electrical properties. Our study promises a new class of ideal superlattices and their great potential as a novel two-dimensional building block for device applications.
Prospective multi-centre evaluation of the incidence of unplanned extubation and its outcomes in intensive care units. The Safe-ICU study
Authors: Guillemin Jérémie , Rieu Benjamin , Huet Olivier , Villeret Léonie , Pons Stéphanie , Bignon Anne , De Roux Quentin , Cinotti Raphaël , Legros Vincent , Plantefeve Gaëtan , Dayhot-Fizelier Claire , Omar Edris , Cadoz Cyril , Bounes Fanny , Caplin Cécile , Toumert Karim , Martinez Thibault , Bouvier Damien , Coutrot Maxime , Godet Thomas , Garçon Pierre , Assefi Mona , Constantin Jean-Michel 
Published: February 2023

Abstract
Background: The lack of a clear definition and strong methodology has led to differing results in terms of unplanned extubation (UE) epidemiology and outcomes. We aimed to determine the UE incidence and clinical significance of both accidental extubation and self-extubation.
Methods: A multicentric prospective cohort study was conducted in 47 French ICUs. The number of mechanical ventilation (MV) days, planned and unplanned extubation were recorded in each centre over a minimum period of three consecutive months to evaluate UE incidence. Patient characteristics, UE environmental factors, and outcomes (follow-up until ICU discharge or day 28) were compared based on the UE mechanism (accidental or self-extubation). Finally, we determined ‘failed’ self-extubation (re-intubation at day 7) prognosis and risk factors.
Results: During the 12-month inclusion period, we found a pooled UE incidence of 1.0 per 100 MV days. UE accounted for 9% of all endotracheal removals. Of the 635 UE, 88% were self-extubations and 12% were accidental extubations. The latter had a worse prognosis than self-extubations (34% vs 14% mortality, p <0.001). Self-extubation failure, which occurred in 35% of self-extubation patients, was associated with excess mortality (15% vs 4%, p <0.001). Cancer, higher respiratory rate, lower PaO2/FiO2, weaning process not ongoing, and immediate post-extubation respiratory failure were independent predictors of failed self-extubation. Discomfort was the leading cause of self-extubation reported by both patients and physicians, ahead of agitation.
Conclusion: Unplanned extubation is common in ICU and accounts for 9% of all endotracheal removals. Accidental extubation has a poorer prognosis than self-extubation.
Effects of Saccharina japonica and spent mushroom compost mixed compost on composting quality and dynamic change of microbial diversity at different C/N ratios
Authors: Song Wanlin , Zhang Hongxia , Zeng Yang , Wu Jiali , Wang Derui , Zhang Yuxue , Feng Dawei 
Published: February 2023

Abstract
Seaweed as a fertilizer for plant growth has been renowned for centuries. The active substances in seaweed can be effectively exploited by land-based co-composting. The efficiency of co-composting can be improved by adjusting the initial C/N ratios of raw materials or adjusting the initial water content. The purpose of this study is to study how the initial C/N ratio in the composting process of a mixture of Saccharina japonica algae and spent mushroom compost residue affects the composting process, explore the most suitable C/N ratio, and determine the physicochemical and biological parameters in the composting process to evaluate the effects of different initial C/N on the composting process. The dynamic changes in microbial diversity were evaluated using high-throughput sequencing at different C/N ratios: 20:1, 30:1, 40:1 and 50:1. 16S rRNA sequencing showed that at 30:1 C/N after composting, the content of Pseudomonas in the experimental group was significantly higher than that in other groups. Pseudomonas is one of the main strains producing alginate lyase, which is beneficial to the degradation of alginate and the release of active substances. Spearman’s correlation analysis showed that pH and alginate significantly affected the succession of dominant bacteria during composting. The results showed that when the C/N ratio was 30:1, this ratio would produce high-quality mature compost.
Using of lepidium sativum L. extract, as antimicrobial and anti-mosquito
Authors: A Emaduldeen H. , Waheeb Mohammed Qasim , Makki Maytham Abas 
Published: February 2023

Abstract
Garden cress (Lepidium sativum L.), a member of the Cruciferae family, is widely planted across the world, especially in India, Europe, and the United States. It has been respected as a key medicinal plant from the time of the Vedic culture. Lepidium sativum Linn. seeds were tested for their efficacy as an antibacterial agent against pathogens found in food. The active components were extracted from the powdered dry seeds using chloroform, ethyl acetate, methanol, and dichloromethane. The antibacterial activity of various doses of the extracts was evaluated using agar well diffusion. We also estimated the MIC and MBC for the most effective extract using the tube dilution technique and the subculturing method, respectively. One of the most common mosquito species that carries Plasmodium falciparum is the Anopheles gambiae sensu lato, which may be combated by sprinkling fields with Lepidium sativum seeds. Scientists have begun to extract essential oils from Lepidium sativum and evaluate their bio-potential against juvenile and adult Anopheles gambiae as part of an attempt to identify ecologically viable vector control tactics. Based on the findings, it is clear that L. sativum essential oil effectively kills An. gambiae. Although field application on a wide scale is necessary for An. gambiae population control, more work has to be done in formulation and assessment.
Marsupials have monoallelic MEST expression with a conserved antisense lncRNA but MEST may not be imprinted.
Authors: Renfree Marilyn , Ishihara Teruhito , Suzuki Shunsuke , Fenelon Jane , Griffith Oliver , Shaw Geoff 
Published: February 2023

Abstract
The imprinted isoform of the Mestgene in mice is involved in key mammalian traits such as placental and fetal growth, maternal care and mammary gland maturation. MEST has a distinct promoter differentially methylated region (DMR) in eutherian mammals but in marsupials, while MEST was thought to be imprinted, it had no DMR. In this study, we examined similarities and differences in the MEST gene locus across mammals using a marsupial, the tammar wallaby, a monotreme, the platypus, and a eutherian, the mouse, to investigate how MEST imprinting evolved in mammals. By confirming the presence of the short isoform in all mammalian groups (which is imprinted in eutherians), this study suggests that an alternative promoter for the short isoform has evolved at the MESTgene locus in the common ancestor of mammals. In the tammar, the short isoform of MEST shared the putative promoter CpG island with an antisense lncRNA previously identified in humans and an isoform of a neighbouring gene CEP41. The antisense lncRNA was expressed in tammar sperm, as seen in humans. This suggested that the conserved lncRNA might play an important part in the establishment of MEST imprinting in therian mammals, but it was not imprinted in the tammar. In contrast to previous studies, this study showed that MEST is non-imprinted and mono-allelically expressed in marsupials. This suggests that selection of MEST imprinting in eutherians must have occurred after the marsupial-eutherian split with the acquisition of a key epigenetic imprinting control region, the DMR.
BacSp222 bacteriocin as a novel ligand for TLR2/TLR6 heterodimer
Authors: Śmiałek-Bartyzel Justyna , Bzowska Monika , Mężyk-Kopeć Renata , Kwissa Marcin , Mak Paweł 
Published: February 2023

Abstract
Objective and design
BacSp222 bacteriocin is a bactericidal and proinflammatory peptide stimulating immune cells to produce selected cytokines and NO in NF-ĸB dependent manner. This study aims to identify the receptor which mediates this activity.
Methods
We applied fluorescently labeled BacSp222 and a confocal microscopy imaging to analyze the direct interaction of the bacteriocin with the cells. Reporter HEK-Blue cells overexpressing human Toll-like receptors (TLR2, TLR4, TLR5 or TLR2/TLR1 and TLR2/TLR6 heterodimers) were stimulated with BacSp222, and then the activity of NF-ĸB-dependent secreted embryonic alkaline phosphatase (SEAP) was measured. In turn, formylated peptide receptor (FPR) or TLR2 antagonists were used to verify bacteriocin-stimulated TNF production by murine monocyte-macrophage cell lines.
Results
BacSp222 undergoes internalization into cells without disturbing the cell membrane. FPR antagonists do not affect TNF produced by BacSp222-stimulated murine macrophage-like cells. In contrast, BacSp222 stimulates NF-ĸB activation in HEK-Blue overexpressing TLR2 or TLR2/TLR6 heterodimer, but not TLR2/TLR1, TLR4 or TLR5 receptors. Moreover, TLR2-specific antagonists inhibit NF-ĸB signalling in BacSp222-stimulated HEK-Blue TLR2/TLR6 cells and reduce TNF release by BacSp222-treated RAW 264.7 and P388.D1.
Conclusions
BacSp222 is a novel ligand for TLR2/TLR6 heterodimer. By binding TLR complex the bacteriocin undergoes internalization, inducing proinflammatory signalling that employs MyD88 and NF-ĸB pathways.
Parental uncertainty in plans and education of Ukrainian refugee children in European countries.
Authors: Chovpan Ganna 
Published: February 2023

Abstract
The contribution of this article shows how uncertainty affects the education vector for Ukrainian refugee parents. The concept of uncertainty is discussed, and in what way the uncertainty affects the educational strategy for the children of Ukrainian refugees. The Syrian children's experience in Turkey is a basis for the study. A survey was conducted among the parents of schoolchildren refugees. It was concluded that among those who are in a state of most significant uncertainty, there is the greatest desire to complete all possible strategies in education for their children. It is necessary to develop more flexible criteria for different groups of refugee children, depending on their age and their parents' plans to return home.
Characterization of the arbuscular mycorrhizal fungal community associated with rosewood (Pterocarpus tinctorius Welw.) in Miombo forests, Democratic Republic of Congo
Authors: Kaumbu Jean-Marc Kyalamakasa , Sene Godar , Stefani Franck , Khasa Damase P. 
Published: February 2023

Abstract
Understanding the dynamics of arbuscular mycorrhizal fungal (AMF) in response to land use change is crucial for the restoration of degraded forests. Here, we describe the diversity and structure of AMF communities in roots of Pterocarpus tinctorius sampled from aluminium- and iron-rich agricultural and forest fallow soils. AMF in root samples were identified by sequencing the large subunit region of the rRNA gene. A total of 30 operational taxonomic units (OTUs) were recovered, most of which were new to science. These OTUs belonged to five genera namely Rhizophagus, Dominikia, Glomus, Sclerocystis and Scutellospora. AMF species richness was significantly influenced by soil properties and tree density, with a low mean value (3.2) observed in acidic soils with high Al and Fe. Indicator species analyses revealed nine AMF OTUs as indicators of base saturation (4 OTUs), high aluminium (3 OTUs) and iron (2 OTUs). OTUs positively correlated with acidity (1 OTU), iron and available phosphorus (2 OTUs) were assigned to the genus Rhizophagus, and indicated Al and Fe tolerance. The results show that leguminous trees in tropical dry forests are a potential reservoir of unknown AM fungal species. The baseline data obtained in this study opens new avenues for future studies, including the use of indigenous AMF-based biostimulants to implement ecological revegetation programs and improve land use.
Aspirin promotes ferroptosis by attenuating Nrf2 in triple-negative breast cancer
Authors: Gou Qiheng , Xie Yuxin , Gou Qitao , Liu Zijian , Ma Ji 
Published: February 2023

Abstract
Purpose
Recent research has found that patients who receive aspirin might have a reduced risk of breast cancer. However, how aspirin influences cancer remains controversial. Ferroptosis is an iron- and reactive oxygen species (ROS)-dependent form of regulated cell death (RCD), and it is crucial for the suppression of tumors. Herein, we investigated the mechanism of aspirin as a novel ferroptosis inducer in the antitumor effect of triple-negative breast cancer (TNBC).
Methods
We performed cell proliferation, ferroptosis-related and xenograft assays to determine the function and mechanism of aspirin in TNBC. Additionally, we investigated the transcriptomic profiles in TNBC tissues and normal tissues by RNA-seq.
Results
Our present study revealed that aspirin not only significantly accelerated ferroptosis but also inhibited the growth of TNBC cells. Intriguingly, aspirin, similar to the ferroptosis activator erastin, promoted ferroptosis and inhibited cell proliferation; however, the additional ferroptosis suppressor ferrostatin reversed the function of aspirin in ferroptosis and cell proliferation. Mechanistically, these results indicated that aspirin attenuated the level of Nrf2 protein and enhanced Keap1, mainly through the Keap1-Nrf2 pathway, in TNBC cells. Meanwhile, aspirin could also suppress key ferroptosis factors, such as GPX4 and xCT. Importantly, restoring Nrf2 signaling reversed aspirin-mediated ferroptosis. Furthermore, we found that oxidative stress and the Keap1-Nrf2 pathway were significantly changed.
Conclusion
Overall, our research demonstrates a novel role of aspirin in inhibiting Keap1-Nrf2 signaling to accelerate ferroptosis, which leads to the inhibition of cell proliferation in TNBC. Aspirin may present as a potential preventive strategy against the development of TNBC.
Expression of E-cadherin by large CD103+ CD69+ CD8+ T cells promotes their invasion into biliary epithelial cells
Authors: Davies Scott , Ronca Vincenzo , Wootton Grace , Bozward Amber , Fiancette Remi , Reynolds Gary , Krajewska Natalia , Osei-Bordom Daniel , Richardson Naomi , Grover Liam , Oo Ye 
Published: February 2023

Abstract
The presence of CD8+ T cells in the cytoplasm of biliary epithelial cells (BEC) has been correlated with biliary damage associated with Primary Biliary Cholangitis (PBC). Here, we characterise the of mechanism of CD8+ T cell entry into BEC. CD8+ T cells observed within BEC were large, eccentric, and expressed E-cadherin, CD103 and CD69. Internalisation required cytoskeletal rearrangements which facilitated contact with BEC. Internalised CD8+ T cells were observed in both non-cirrhotic and diseased liver tissues but enriched in PBC patients. E-cadherin expression by CD8+ T cells correlated with frequency of internalisation of these cells into BEC. E-cadherin+ CD8+ T cells formed β-catenin-associated interactions with BEC. E-cadherin+ CD8+ T cells were larger than E-cadherin- CD8+ T cells and invaded BEC more frequently. Overall, we unveil a distinct cell-in-cell structure in the liver detailing the invasion of E-cadherin+ CD103+ CD69+ CD8+ cells T cells into BEC.
Ropivacaine enhanced the cisplatin-sensitivity of Human colorectal cancer cells by accelerating ROS-meditated apoptosis and ferroptosis
Authors: Zhang Zhen , Zeng Lian , Zhao WenBo , Zhao Qiang , Wang Qingsong , Ding Xudong , Xu Qianqian , Zhang Yu , Hu Pengchao , Luo Huiyu 
Published: February 2023

Abstract
The failure of cisplatin treatment to human colorectal cancer due to cisplatin-sensitivity reduced, it’s necessary to find the new adjuvant agents to increase the cisplatin-sensitivity. Ropivacaine has been demonstrated to inhibit the proliferation and migration of variety of cancer cells, however, it remains unclear whether it could increase the cisplatin sensitivity of colorectal cancer cells. The cisplatin-resistant cell line for colorectal cancer named LOVO/DDP cells were established by repeated induction of different concentration of cisplatin, the cell viability, proliferation, migration, and apoptosis were assessed by Colony-forming assay, Transwell assay, Wound Healing and Annexin V/PI flow cytometry; JC-1 and ROS detection kits were used to determine the levels of mitochondrial membrane potential and reactive oxygen species (ROS). The expression of MMP-9, Nrf-2, GPX4, SLC7A11, SIRT1 proteins were detected by Western-blot and immunofluorescence. Compared with the control group, the activity of LOVO cells decreased significantly and that in LOVO/DDP cells decreased slightly with the increase of cisplatin. After the treatment of ropivacaine combined with cisplatin, the LOVO/DDP cells viability was significantly decreased than cisplatin alone (P<0.01). Compared with cisplatin alone treatment group, the proliferation and migration of LOVO cells and LOVO/DDP cells were significantly reduced in combined group. Ropivacaine combined with cisplatin enhanced apoptosis, the production of ROS, induced mitochondrial dysfunction, and down-regulated anti-ferroptosis and SIRT1 proteins. Those above results demonstrated that ropivacaine could increase the chemosensitivity of cisplatin-resistant human colon cancer cells, and its mechanism may be through promoting cancer cell apoptosis and ferroptosis.
First evidence of free-living Naegleria species in recreational lakes of Alberta, Canada
Authors: Dey Rafik , Dlusskaya Elena , Oloroso Mariem , Ashbolt Nicholas J. 
Published: February 2023

Abstract
Rising temperatures are increasing environmental habitats for thermotolerant pathogens, such as the so-called ‘brain-eating amoeba’, Naegleria fowleri. To the best of our knowledge, however, Naegleria species have not been reported in environmental water sources in Canada. We surveyed popular recreational lakes in Alberta, Canada during the summer bathing period to determine the presence or absence of Naegleria species. While N. fowleri was not isolated in this study, we identified other thermotolerant species, including Naegleria pagei, Naegleria gruberi, Naegleria jejuensis and Naegleria fultoni using culture-based methods, hence indicating the potential conditions to support N. fowleri. Ongoing monitoring and examination of water for pathogenic amoebae is recommended in order to assist in the public health management of water sources.
An improved ‘assessment on comprehensive index’ for drought risk through an iterative transformation of ‘spatiotemporal vector’: a case study of Anhui Province, China
Authors: Tang Ming , Xu Wentao , Xie Qianchen , Wu Yuze , Li Yanlei , Zhang Huanwei 
Published: February 2023

Abstract
The unique climate, topography and soil conditions of Anhui Province in China are the main causes of drought, which is one of the limiting factors of economic and social development. Characterized by low data requirement, strong operability and flexibility, the conventional ‘assessment based on comprehensive index’ (ACI) is used to assess the drought risk, the index obtained by which can only represent the relative risk level among the objects and has no spatiotemporal characteristic. Therefore, a new method based on the iterative transformation of spatiotemporal vector (DRST) is proposed to solve this defect. The main conclusions are as follows: (1) The drought risk matrices obtained by DRS and DRT cannot quantify the drought risk level of subregions in each year absolutely. (2) The drought risk values obtained by DRST can be used to compare the drought risk from temporal and spatial dimensions at the same time, and both the drought-affected regions and the drought risk level are consistent with the statistical data. The new method expands the subsequent utilization space of the ‘assessment index’. For example, the allocation of drought-resistant resources (water resources, drought-resistant funds, etc.) can be determined according to these indexes.
Analysis of potential nature-based solutions for the Mun River Basin, Thailand
Authors: Penny Jessica , Alves Priscila B. R. , De-Silva Yenushi , Chen Albert S. , Djordjević Slobodan , Shrestha Sangam , Babel Mukand 
Published: February 2023

AbstractDespite the growth in research and applications of nature-based solutions (NBS) within the literature, there are limited applications in South East Asia, moreover studies which quantitatively assess the impacts of NBS could have on hazard reduction are scarce. This paper addresses this gap by developing and validating MCDA-GIS analysis to map how potential nature strategies could mitigate flood hazard if applied within the Mun River Basin, Thailand. Through a literature review, the top three solutions for flood and drought hazards were found: wetlands, re/afforestation, and changing crop types. These strategies were reviewed and validated with a MCDA-GIS methodology, through land use change (LUC) maps to depict different future scenarios. The results found that flood hazard did decrease when NBS were implemented in the catchment, especially for A/Reforestation, and to a greater extent when a combination of NBS were applied. This article provides specific insights into the current gaps of NBS publications, specifically considering the case of the Mun River Basin, Thailand.
Abrocitinib: First Globally Approved Selective Janus Kinase-1 Inhibitor
for the Treatment of Atopic Dermatitis
Authors: De Surya K. 
Published: February 2023

Abstract:
Atopic dermatitis is epidermal hyperplasia, skin barrier dysfunction, and the
aberrant activation of immune cells. Janus kinase (JAK) is a family of cytoplasmic nonreceptor
tyrosine kinases that consists of four members, such as JAK1, JAK2, JAK3, and
TYK2. The JAK signaling pathway plays a critical role in a wide range of autoimmune
and inflammatory diseases, including atopic dermatitis. Abrocitinib is an orally bioavailable
and selective JAK1 inhibitor, and it was approved in January, 2022, for the treatment
of atopic dermatitis. The chemical structure and physical properties of abrocitinib, its synthesis,
mechanism of action, and pharmacokinetic profile are summarized.
Current Strategies for Promoting the Large-scale Production of
Exosomes
Authors: Qu Qing , Fu Bin , Long Yong , Liu Zi-Yu , Tian Xiao-Hong 
Published: February 2023

Abstract:
Exosomes, as nanoscale biological vesicles, have been shown to have great potential for biomedical
applications. However, the low yield of exosomes limits their application. In this review, we
focus on methods to increase exosome yield. Two main strategies are used to increase exosome production,
one is based on genetic manipulation of the exosome biogenesis and release pathway, and the
other is by pretreating parent cells, changing the culture method or adding different components to the
medium. By applying these strategies, exosomes can be produced on a large scale to facilitate their
practical application in the clinic.