bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Preoperative Design to Address Dermatochalasis in Asian Upper Blepharoplasty
Authors: Liu Qing , Wang Zhaohui , Yang Juanmin , Wu Shu , Zhang Youlai , Gan Peidong , Yi Yangyan 
Published: February 2023

Background:
The preoperative design for the amount of skin excision of the upper eyelid is a common procedure in Asian blepharoplasty, but there lack of an effective method addressing dermatochalasis to acquire esthetically pleasing results. Our aim was to propose an alternative technique to determine the skin excision combined with the esthetics of eyes for correcting skin laxity and therefore to create attractive double eyelids.

Materials and Methods:
The preoperative invaginating-simulating design combined with esthetic criteria for determining the amount of excised skin were performed during blepharoplasty. The Strasser grading system and the Global Aesthetic Improvement Scale were evaluated by analyzing the preoperative and 6-month-postoperative photographs.

Results:
One hundred forty-two patients were included. The general outline of the “optimal incision” took on a knife in shape with a mean of 2.2±3.32 points of Strasser grading score. A total of 130 of 142 patients (91.5%) were judged as “good results” and 12 of 142 patients (8.5%) were judged as “mediocre”. The Global Aesthetic Improvement Scale showed a significant cosmetic improvement with the result of 89.4%(127 of 142 patients) for “very much improved”, 7.8% for “much improved” and other patients for “improved”. No severe complications were observed.

Conclusions:
This preoperative design approach has been proven to be effective to address dermatochalasis (especially for lateral hooding) by simulating the expected appearance of a double eyelid combined with esthetic criteria of eyes simultaneously, which can contribute to achieving upper-lid rejuvenation and beautiful and natural outcomes.
Perioperative Medication Management in Elective Plastic Surgery Procedures
Authors: Chaya Bachar F. , Rodriguez Colon Ricardo , Boczar Daniel , Daar David , Brydges Hilliard , Thys Erika , Kantar Rami , Saadeh Pierre B. 
Published: February 2023

Background:
Perioperative medication management is vital to maintain patient safety while under anesthesia, as well as to avoid postoperative complications. Plastic surgeons make daily decisions on whether to ask a patient to stop taking medication before their surgery. These important decisions can affect bleeding risk, wound healing, and interactions with anesthetics, which can range from minor to life-threatening. Current plastic surgery literature lacks a comprehensive review of perioperative medication management, with existing reports focusing on specific procedures and specific medication classes.

Methods:
A PubMed database search was conducted for articles through July 2021. The bibliographies of included studies were also examined for articles not acquired in the initial search queries. The authors included studies on medication usage and perioperative guidance in patients undergoing elective plastic surgery procedures. The authors excluded studies unrelated to plastic surgery and studies where the medications were used as an intervention. Abstracts, animal studies, studies involving the pediatric population, and book chapters were also excluded, as well as articles not published in English.

Results:
A total of 801 papers were identified by our search terms. After title and abstract screening, 35 papers were selected for full-text review. After full-text review, 20 papers were selected for inclusion, with an additional 6 papers from cited references added. Of the 26 papers, 6 papers discussed psychotropic drugs, 6 papers discussed medications affecting hemostasis, 4 papers discussed hormone-containing medications, 3 papers discussed antilipid medications, 2 papers discussed antihypertensive medications, 2 papers discussed herbal supplements, 1 paper discussed both psychotropic and herbal supplements, 1 paper discussed medications affecting wound healing, and 1 paper discussed rheumatologic medications. A summary of those recommendations was then compiled together.

Conclusions:
The perioperative medication management in elective plastic surgery procedures remains a complex and multidisciplinary process. It is important to manage these patients in a case-by-case manner and to consult a specialist when necessary. Careful medication reconciliation is essential to decrease the likelihood of adverse outcomes and interactions with perioperative anesthetics.
Lung Transplant Outcome From Selected Older Donors (≥70 Years) Equals Younger Donors (<70 Years)
Authors: Vanluyten Cedric , Vandervelde Christelle M. , Vos Robin , Van Slambrouck Jan , Fieuws Steffen , De Leyn Paul , Nafteux Philippe , Decaluwé Herbert , Van Veer Hans , Depypere Lieven , Jansen Yanina , Provoost An-Lies , Neyrinck Arne P. , Ingels Catherine , Vanaudenaerde Bart M. , Godinas Laurent , Dupont Lieven J. , Verleden Geert M. , Van Raemdonck Dirk , Ceulemans Laurens J. 
Published: February 2023

Objective:
To describe our experience with lung transplantation (LTx) from donors ≥70 years and compare short and long-term outcomes to a propensity-matched cohort of donors <70 years.

Background:
Although extended-criteria donors have been widely used to enlarge the donor pool, the experience with LTx from older donors (≥70 years) remains limited.

Methods:
All single-center bilateral LTx between 2010 and 2020 were retrospectively analyzed. Matching (1:1) was performed for the donor (type, sex, smoking history, x-ray abnormalities, partial pressure of oxygen/fraction of inspired oxygen ratio, and time on ventilator) and recipient characteristics (age, sex, LTx indication, perioperative extracorporeal life support, and cytomegalovirus mismatch). Primary graft dysfunction grade-3, 5-year patient, and chronic lung allograft dysfunction-free survival were analyzed.

Results:
Out of 647 bilateral LTx, 69 were performed from donors ≥70 years. The mean age in the older donor cohort was 74 years (range: 70–84 years) versus 49 years (range: 12–69 years) in the matched younger group. No significant differences were observed in the length of ventilatory support, intensive care unit, or hospital stay. Primary graft dysfunction-3 was 26% in the older group versus 29% in younger donor recipients (P = 0.85). Reintervention rate was comparable (29% vs 16%; P = 0.10). Follow-up bronchoscopy revealed no difference in bronchial anastomotic complications (P = 1.00). Five-year patient and chronic lung allograft dysfunction-free survivals were 73.6% versus 73.1% (P = 0.72) and 51.5% versus 59.2% (P = 0.41), respectively.

Conclusions:
LTx from selected donors ≥70 years is feasible and safe, yielding comparable short and long-term outcomes in a propensity-matched analysis with younger donors (<70 years).
Magnetic Resonance Imaging Predictors of Hepatocellular Carcinoma Progression and Dropout in Patients in Liver Transplantation Waiting List
Authors: Baghdadi Azarakhsh , Luu Harry T. , Shaghaghi Mohammadreza , Ghadimi Maryam , Simsek Cem , Xu Ziyi , Hazhirkarzar Bita , Motaghi Mina , Hammami Muhammad , Clark Jeanne M. , Gurakar Ahmet , Kamel Ihab R. , Kim Amy K. 
Published: February 2023

Background.
With the rising incidence of hepatocellular carcinoma (HCC), more patients are now eligible for liver transplantation. Consequently, HCC progression and dropout from the waiting list are also anticipated to rise. We developed a predictive model based on radiographic features and alpha-fetoprotein to identify high-risk patients.

Methods.
This is a case-cohort retrospective study of 76 patients with HCC who were listed for liver transplantation with subsequent liver transplantation or delisting due to HCC progression. We analyzed imaging-based predictive variables including tumor margin (well- versus ill-defined), capsule bulging lesions, volumetric analysis and distance to portal vein, tumor numbers, and tumor diameter. Volumetric analysis of the index lesions was used to quantify index tumor total volume and volumetric enhancement, whereas logistic regression and receiver operating characteristic curve (ROC) analyses were used to predict the main outcome of disease progression.

Results.
In univariate analyses, the following baseline variables were significantly associated with disease progression: size and number of lesions, sum of lesion diameters, lesions bulging the capsule, and total and venous-enhancing (viable) tumor volumes. Based on multivariable analyses, a risk model including lesion numbers and diameter, capsule bulging, tumor margin (infiltrative versus well-defined), and alpha-fetoprotein was developed to predict HCC progression and dropout. The model has an area under the ROC of 82%, which was significantly higher than Milan criteria that has an area under the ROC of 67%.

Conclusions.
Our model has a high predictive test for patient dropout due to HCC progression. This model can identify high-risk patients who may benefit from more aggressive HCC treatment early after diagnosis to prevent dropout due to such disease progression.
Application of Ex Vivo Normothermic Machine Perfusion in Deceased Donors With Acute Kidney Injury With Successful Renal Transplantation: A Preliminary Experience
Authors: Choudhary Devprakash , Sharma Ashish , Singh Sarbpreet , Kenwar Deepesh B. , Walker Minz Ranjana , Singh Kohli Harbir , Nada Ritambhra , Wangkheimayum Sujata , Jain Kajal , Patil Shivakumar S. 
Published: February 2023

Background.
Ex vivo normothermic machine perfusion (NMP) has improved organ preservation and viability assessment among heart, liver, and lung transplantation. However, literature regarding the application of NMP in human clinical kidney transplantation remains limited. Numerous kidneys, especially from donors with stage 3 acute kidney injury (AKI), are not utilized concerning the high rate of delayed graft function (DGF) and primary nonfunction. The present study investigated the impact of NMP (135–150 min) on short-term outcomes after kidney transplantation from deceased donors with AKI.

Methods.
Graft outcomes of NMP kidneys were compared with contralateral kidneys stored in static cold storage (SCS) from the same donor with AKI during December 2019–June 2021. The study’s primary aim is to assess the safety and feasibility of NMP in deceased donors with AKI. The primary outcome was DGF. Secondary outcomes were duration of DGF, biopsy-proven rejection, postoperative intrarenal resistive index, postoperative infections, hospital stay duration, primary nonfunction, and kidney function estimated glomerular filtrate rate at discharge, 3 mo, and 1 y.

Results.
Five pairs of AKI kidneys (NMP versus SCS) were included in the final analysis. The results show no statistically significant differences in clinical outcomes between NMP versus SCS kidneys; however, NMP kidneys demonstrated slightly improved estimated glomerular filtrate rate at 3 mo (59.8 ± 5.93 [59] versus 75.20 ± 14.94 [74]) mL/min/1.73 m2 (P < 0.065) and at the last follow-up (12–29 mo) (72.80 ± 10.71 [75]) versus (94 ± 22.67 [82]) mL/min/1.73 m2 (P < 0.059) as compared with SCS kidneys. A higher proportion of NMP kidneys had normal intrarenal resistive index (0.5–0.7) and mild acute tubular injury on protocol biopsy, suggesting NMP is safe and feasible in deceased donors with acute kidney injury.

Conclusions.
NMPs of AKI donor kidneys are safe and feasible. A larger cohort is required to explore the reconditioning effect of NMP on AKI kidneys.
Comparison of the Immune Response After an Extended Primary Series of COVID-19 Vaccination in Kidney Transplant Recipients Receiving Standard Versus Mycophenolic Acid–sparing Immunosuppressive Regimen
Authors: Phirom Supitchaya , Phannajit Jeerath , Jantarabenjakul Watsamon , Paitoonpong Leilani , Kitrungphaiboon Thidarat , Choktaweesak Nuchjarnun , Kupatawintu Pawinee , Wattanatorn Salin , Prasithsirikul Wisit , Eiam-Ong Somchai , Avihingsanon Yingyos , Hansasuta Pokrath , Vanichanan Jakapat , Townamchai Natavudh 
Published: February 2023

Background.
Two doses of coronavirus disease 2019 vaccination provide suboptimal immune response in transplant patients. Mycophenolic acid (MPA) is one of the most important factors that blunts the immune response. We studied the immune response to the extended primary series of 2 doses of AZD1222 and a single dose of BNT162b2 in kidney transplant patients who were on the standard immunosuppressive regimen compared to those on the MPA-sparing regimen.

Methods.
The kidney transplant recipients who were enrolled into the study were divided into 2 groups based on their immunosuppressive regimen. Those on the standard immunosuppressive regimen received tacrolimus (TAC), MPA, and prednisolone (standard group). The patients in the MPA-sparing group received mammalian target of rapamycin inhibitors (mTORi) with low dose TAC plus prednisolone (MPA-sparing group). The vaccination consisted of 2 doses of AZD1222 and a single dose of BNT162b2.

Results.
A total of 115 patients completed the study. There were 76 (66.08%) patients in the standard group and 39 (33.91%) patients in the MPA-sparing group. The overall median anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) S antibody level at 4 wk after vaccine completion was 676.64 (interquartile range = 6.02–3644.03) BAU/mL with an 80% seroconversion rate. The MPA-sparing group achieved higher anti–SARS-CoV-2 S antibody level compared to the standard group (3060.69 and 113.91 BAU/mL, P < 0.001). The seroconversion rate of MPA-sparing and standard groups were 97.4% and 71.1%, respectively (P < 0.001). The anti-HLA antibodies did not significantly increase after vaccination.

Conclusions.
The extended primary series of 2 doses of AZD1222 and a single dose of BNT162b2 provided significant humoral immune response. The MPA-sparing regimen with mTORi and low dose TAC had a higher ant–SARS-CoV-2 S antibody level and seroconversion rate compared to the participants in the standard regimen.
Seizure Occurring During Baclofen Monotherapy for Phenibut Withdrawal
Authors: Patt Amber , Fox Haley , Wells Lauren , Theobald Jillian , Feldman Ryan 
Published: February 2023

Objective
Phenibut is a widely available gamma-aminobutyric acid B receptor agonist. When taken chronically, phenibut causes dependence and subsequent withdrawal if stopped. Baclofen, a gamma-aminobutyric acid B receptor agonist structurally related to phenibut, has been used to manage phenibut withdrawal (PW), although baclofen doses for PW are not well defined and may exceed Food and Drug Administration–approved doses. Little data described outcomes from baclofen use.

Methods
This case details a patient who experienced a seizure while on baclofen 10 mg thrice daily as monotherapy for PW and highlights a potential risk of underdosing baclofen as monotherapy in the management of PW.

Results
A man in his early 30s with anxiety, depression, and substance use disorder presented to the emergency department by family for lethargy and confusion starting earlier that day. He had been using 25–30 g of phenibut daily for the past 6 months. On arrival, he was hypertensive, tachycardic, tachypneic, and lethargic. The patient received 1 mg of intravenous lorazepam and was admitted to the hospital for presumed PW. His symptoms improved overnight, and he was discharged on 10 mg of baclofen thrice daily. He returned 28 hours later after having a seizure and required intensive care admission in addition to multimodal drug therapy.

Conclusions
Phenibut use is rising, and treatment options for PW, such as baclofen, warrant additional study. Potential risks of underdosing baclofen if used as monotherapy in PW may include seizures as withdrawal progresses. Baclofen's role in therapy may be more appropriate as an adjunct than a cornerstone of therapy. Treatment done in consultation with providers experienced in managing withdrawal such as a toxicologist may help reduce this risk.
Knowing When to Ignore the Numbers: Single Center Experience Transplanting Deceased Donor Kidneys with Poor Perfusion Parameters
Authors: Werenski Hope , Stratta Robert J , Sharda Berjesh , Garner Matthew , Farney Alan C , Orlando Giuseppe , McCracken Emily , Jay Colleen L 
Published: February 2023

Background:
Hypothermic machine perfusion is frequently utilized in evaluating marginal kidneys, with poor perfusion parameters (PPP) contributing to delays in kidney placement or discard. We examined outcomes in deceased donor kidney transplants (DDKT) with PPP compared to those with optimal perfusion parameters (OPP).

Study Design:
We conducted a retrospective single center cohort study from 2001 to 2021 comparing PPP (n=91) to OPP (n=598) DDKT. PPP was defined as terminal flow ≤80 ml/min AND terminal resistance ≥0.40 mmHg/ml/min. OPP was defined as terminal flow ≥120 ml/min AND terminal resistance ≤0.20 mmHg/ml/min.

Results:
Mean terminal flow was PPP 66 ± 16 vs OPP 149 ± 21 ml/min and resistance was PPP 0.47 ± 0.10 vs OPP 0.15 ± 0.04 mmHg/ml/min (both p<0.001). Donor age, donation after cardiac death, and terminal serum creatinine levels were similar between groups. Mean Kidney Donor Profile Index (KDPI) was higher among PPP donors (PPP 65 ± 23% vs OPP 52 ± 27%, p<0.001). The PPP transplant group had more females and lower weight and BMI. Delayed graft function was comparable (PPP 32% vs OPP 27%, p=0.33) even though cold ischemia times (CIT) trended toward longer in PPP kidneys (PPP 28 ± 10 vs OPP 26 ± 9 hours, p=0.09). One-year patient survival (PPP 98% vs OPP 97%, p=0.84) and graft survival (PPP 91% vs OPP 92%, p=0.23) were equivalent. PPP did predict inferior overall and death-censored graft survival long-term (overall HR=1.63, 95%CI=1.19-2.23 and death-censored HR=1.77, 95%CI=1.15-2.74). At 1 year, eGFR was higher with OPP kidneys (PPP 40 ± 17 vs OPP 52 ± 19 ml/min/1.73m2, p<0.001).

Conclusion:
Short-term outcomes in PPP kidneys were comparable to OPP kidneys despite higher KDPI and longer CIT, suggesting a role for increased utilization of these organs with careful recipient selection.
Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil
Authors: Jin Huijuan , Huo Leitao , Yang Yi , Lv Yan , Wang Jingjing , Maillard Julien , Holliger Christof , Löffler Frank E. , Yan Jun 
Published: February 2023

Two anaerobic, tetrachloroethene- (PCE-) respiring bacterial isolates, designated strain ACSDCE
T and strain ACSTCE, were characterized using a polyphasic approach. Cells were Gram-stain-negative, motile, non-spore-forming and shared a vibrioid- to spirillum-shaped morphology. Optimum growth occurred at 30 °C and 0.1–0.4 % salinity. The pH range for growth was pH 5.5–7.5, with an optimum at pH 7.2. Hydrogen, formate, pyruvate and lactate as electron donors supported respiratory reductive dechlorination of PCE to cis-1,2-dichloroethene (cDCE) in strain ACSDCE
T and of PCE to trichloroethene (TCE) in strain ACSTCE. Both strains were able to grow with pyruvate under microaerobic conditions. Nitrate, elemental sulphur, and thiosulphate were alternative electron acceptors. Autotrophic growth was not observed and acetate served as carbon source for both strains. The major cellular fatty acids were C16 : 1
ω7c, C16 : 0, C14 : 0 and C18 : 1
ω7c. Both genomes feature a circular plasmid. Strains ACSDCE
T and ACSTCE were previously assigned to the candidate species 'Sulfurospirillum acididehalogenans'. Here, based on key genomic features and pairwise comparisons of whole-genome sequences, including average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, strains ACSDCE
T and ACSTCE, 'Ca. Sulfurospirillum diekertiae' strains SL2-1 and SL2-2, and the unclassified

Sulfurospirillum

sp. strain SPD-1 are grouped into one distinct species separate from previously described

Sulfurospirillum

species. Compared to

Sulfurospirillum multivorans

and

Sulfurospirillum halorespirans

, which dechlorinate PCE to cDCE without substantial TCE accumulation, these five strains produce TCE or cDCE as the end product. In addition, some cellular fatty acids (e.g., C16 : 0 3OH, C17 : 0 iso 3OH, C17 : 0 2OH) were detected in strains ACSDCE
T and ACSTCE but not in other

Sulfurospirillum

species. On the basis of phylogenetic, physiological and phenotypic characteristics, 'Ca. Sulfurospirillum acididehalogenans' and 'Ca. Sulfurospirillum diekertiae' are proposed to be merged into one novel species within the genus

Sulfurospirillum

, for which the name

Sulfurospirillum diekertiae

sp. nov. is proposed. The type strain is ACSDCE
T (=JCM 33349T= KCTC 15819T=CGMCC 1.5292T).
Identification of rare missense variants reducing cathepsin O secretion in families with intracranial aneurysm
Authors: Freneau Milène , Blanchet Raphael , Benichi Sandro , Mrad Mary-Adel , Batta Surya Prakash Rao , Rio Marc , Bonnaud Stéphanie , Lindenbaum Pierre , Laporte Fabien , Cuénot Stéphane , Quillard Thibaud , Morel Sandrine , Kwak Brenda R. , Bijlenga Philippe , Deleuze Jean-François , Dina Christian , Bodet Maxence , Chatel Stéphanie , Bourcereau Emmanuelle , Jouan Solène , Consoli Arturo , Dargazanli Cyril , Ognard Julien ,   ICAN Study Group, Desal Hubert , Vion Anne-Clémence , Bourcier Romain , Loirand Gervaise , Redon Richard 
Published: February 2023

ABSTRACTIntracranial aneurysm (IA) is a common cerebrovascular abnormality characterized by localized dilation and wall thinning in intracranial arteries, which can rupture and lead to fatal subarachnoid hemorrhage. Although the pathophysiology of IA remains largely unknown, increasing evidence suggests that genetic susceptibility plays a predominant role. Here, we combined whole exome sequencing and identity-by-descent analyses with functional investigations to identify rare functional variants associated to IA in families with multiple affected subjects. We identified two rare missense variants in theCTSOgene in two large pedigrees. We found that the cysteine-type papain-like cathepsin O (CTSO) encoded byCTSOis expressed in the wall of human IA domes. Stretching of vascular smooth muscle cells (VSMC) induced secretion of CTSO, which acted as an extracellular protease controlling VSMC migration and adhesion to the extracellular matrix. CTSO depletion, as well as expression of the two CTSO variants, which are poorly secreted, increased the amount of fibronectin. In addition, CTSO depletion increased VSMC stiffness, which was reduced by the addition of exogenous CTSO. Collectively, our findings identify CTSO as a potential new player in arterial remodeling, regulating fibronectin deposition and VSMC function, supporting the causal role of rare codingCTSOvariants in familial forms of IA.