bluuebunny/crossref_metadata_2025_split huggingface dataset:
T Cell-mediated Immune Response and Correlates of Inflammation and their relationship with COVID-19 clinical severity: not an intuitive guess.
Authors: Mantovani Nathalia , Santana Luiz , Hunter James , Blum Vinicius , Vergara Tania , Gouvea Celso , Leal Elcio , Bellei Nancy , Schechter Mauro , Diaz Ricardo 
Published: May 2023

Abstract
Background: Predictors of the outcome of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov-2) infection remain to be fully determined. We evaluated selected viral characteristics and immunological responses that might predict and/or correlate to the clinical outcome of COVID-19.
Methods: The magnitude and breadth of T cell-mediated responses were measured within 36 hours of symptom onset for individuals developing divergent clinical outcomes. Peripheral Blood Mononuclear Cells (PBMCs) were subjected to in vitro stimulation with SARS-CoV-2-based peptides. In addition, SARS-CoV-2 sequences were generated by metagenome, and HLA typing was performed using Luminex technology.
Findings: CD4+ T cell activation was found to be negatively correlated with SARS-CoV-2 basal viral load in patients with severe COVID-19 (p = 0·043). The overall cellular immune response, as inferred by IFN-γ signal, was higher at baseline for patients that progressed to mild disease compared to patients that progressed to severe disease (p = 0·0044). Subjects with milder disease developed higher T cell responses for MHC class I and II-restricted peptides (p = 0·033).
Interpretation: Mounting specific cellular immune responses in the first days after symptom onset, as inferred by IFN-γ magnitude in the ELISPOT assay, may efficiently favor a positive outcome. In contrast, progression to severe COVID-19 was accompanied by stronger cellular immune responses, higher CD4+ T cell activation, and a higher number of in silico predicted high-affinity class I HLA alleles.
Funding: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) - Grant 2020/10396-2, and Conselho Nacional de Desenvolvimento Científico e Tecnológico - Grant 441817/2018-1.
Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly
Authors: Jimaja Sètuhn , Chadwick Robert , Glynn Christopher , Chami Mohamed , Happel Dominic , Guo Chao , Kolmar Harald , Bruns Nico , Belluati Andrea 
Published: May 2023

Abstract
Artificial cells are biomimetic microstructures that mimic functions of natural cells and find application, e.g., as microreactors, as building blocks for molecular systems engineering, and to host synthetic biology pathways. Here, we report enzymatically synthesised polymer-based artificial cells with the ability to express proteins. They are created by biocatalytic atom transfer radical polymerization-induced self-assembly (bioPISA). The metalloprotein myoglobin synthesises amphiphilic block copolymers that self-assemble into structures ranging from micelles over worm-like micelles to polymersomes and giant unilamellar vesicles (GUVs). The GUVs encapsulate cargo during the polymerisation, including enzymes, nanoparticles, microparticles, plasmids and cell lysate. The resulting artificial cells act as microreactors for enzymatic reactions and for osteoblast-inspired biomineralization, and could express proteins when fed with amino acids, as shown by the expression of the fluorescent protein mClover and of actin. Actin polymerises in the vesicles and alters the artificial cell’s internal structure by creating internal compartments. Thus, bioPISA-derived GUVs mimic bacteria as they are composed of a microscopic reaction compartment that contains genetic information which is able to express proteins upon induction. bioPISA not only is a powerful tool in the pursuit of artificial cells but also for the easy and highly efficient encapsulation of biological molecules under mild conditions and in biologically relevant media. Therefore, it could have significant implications for the development of biomaterials and drug-delivery systems, as well as for cell encapsulation, and the in-situ formation of nano-objects.
Alfalfa/maize intercropping has comparable productivity and lower environmental impact than nitrogen fertilizer application
Authors: Zhang Bing , Nasar Jamal , Dong Siqi , zhou xue , Gao Qiang 
Published: May 2023

Abstract
Background and Aims
Due to the unique biological nitrogen fixation of legume crops, intercropping maize with legume/grass crops such as alfalfa becomes an effective way to reduce exogenous nitrogen fertilizer inputs. This study aimed to determine whether intercropping could be compared to nitrogen fertilizer application and identify potential differences.
Methods
The trial was laid out in the field since 2015 in a randomized complete block design. Treatments included maize monocropping without nitrogen (N0M) and with nitrogen (NM), maize/alfalfa intercropping without nitrogen (N0IM). In autumn 2019, crop and rhizosphere soil samples were collected to determine yield, rhizosphere soil properties and microbial indicators.
Results
Results demonstrated that N0IM and NM treatments had the similar effect on the enhancement of maize yield and yield composition factors and the improvement of soil physicochemical properties related to nitrogen and kalium. Additionally, NM treatment significantly increased the Chao1 and ACE indices by 28.1% and 29.49%, while the N0IM treatment significantly increased the Shannon index by 1.90%. The NM and N0IM treatments had significantly different pathways for increasing maize yield. Both NM and N0IM treatments increased the relative abundance of assimilatory nitrate reduction. And the N0IM treatment increased nitrogen fixation as well. At the same time, NM significantly increased nitrogen dissimilation by 23.98% and intercropping significantly increased denitrification by 12.81%.
Conclusion
Intercropping can be considered comparable to nitrogen fertilizer application in terms of yield and yield composition factors, rhizospheric soil physicochemical properties. Moreover, intercropping was found to be more conducive to the stability of rhizospheric soil bacteria and more environmentally friendly.
Hemoadsorption therapy for critically ill patients with acute liver dysfunction: A meta- analysis and systematic review
Authors: Turan Caner , Szigetvary Csenge , Kói Tamás , Engh Marie Anne , Atakan Isil , Zubek László , Terebessy Tamas , Hegyi Peter , Molnár Zsolt 
Published: May 2023

Abstract
Introduction
Critically ill patients admitted to the ICU have been shown to be at risk of developing acute liver dysfunction usually as part of multiorgan failure sequelae. Clearing the blood from toxic liver-related metabolites and cytokines could prevent further organ damage. Despite the increasing use of hemoadsorption for this purpose, evidence of its efficacy is lacking.
Objectives
To appraise the evidence for the efficacy of hemoadsorption (HA) treatment for critical illness-associated acute liver dysfunction.
Methods
A systematic search was conducted on Pubmed, Embase, Scopus, CENTRAL, and Web of Science (PROSPERO registration: CRD42022286213). The target population was patients with acute liver dysfunction or failure associated with critical illness and treated with HA. Outcomes of interest were pre- and post-treatment changes in organ functions and mortality.
Results
The search yielded 30eligible publications between 2011 and 2023, which reported the use of HA for a total of 335patients who presented with liver dysfunction related to acute critical illness. Of those, 26are case presentations (n=84), three are observational studies (n=142) and one is a registry analysis (n=109). Analysis of data from individual cases showed a significant reduction in levels of AST (p = 0.03) and vasopressor need (p = 0.03), and a tendency to lower levels of total bilirubin, ALT,CRP, and creatinine. Pooled data showed a significant reduction in total bilirubin (mean difference of -4.79 mg/dL [95% CI: -6.25; -3.33], p=0.002). In the case of creatinine, the post-treatment mean values were smaller as well; however, the difference was not significant (mean difference: -0.38mg/dL [95% CI:-1.27; 0.5],p=0.203). Evidence is lacking on the effect of HA on mortality. No device-related adverse events were reported.
Conclusions
The use of HA for critically ill patients with acute liver dysfunction or failure seems to be safe and yields a trend towards improved liver function after therapy, but more high-quality evidence is crucially needed.
The effects of motivational interviews about activities of daily living on physical adjustment and quality of life in elderly total knee arthroplasty patients: A randomised-controlled trial
Authors: BAKİR GİZEM KUBAT , GÖKTAS SONAY 
Published: May 2023

Abstract
Introduction:
This study was carried out to investigate the effects of motivational interviews about activities of daily living on physical adjustment and quality of life in elderly total knee arthroplasty patients.
Methods
This study is a randomised-controlled trial. This study was carried out with 70 patients who underwent total knee arthroplasty. The patients were randomly divided into the intervention group where motivational interviews were held in eight sessions (n = 35) and the control group where routine nursing practices were followed (n = 35). The motivational interview program was applied to the patients in the intervention group for three months. The data of the study were collected using a “Patient Information Form”, a “Postoperative Complication Assessment Form”, the “Functional Assessment Form”, and the “SF-36 Quality of Life Questionnaire”.
Results
It was determined that the difference between the mean scores of the Functional Evaluation Form and the SF-36 Quality of Life Questionnaire before the motivational interview of the patients in the experimental and control groups was not statistically significant (p > 0.05). After the motivational interview, the difference between the Functional Evaluation Form mean scores and the Quality of Life Scale mean scores of the patients in the experimental group was found to be statistically significant (p < 0.05).
Conclusion
It was concluded that motivational interviews about activities of daily living increase physical adjustment and quality of life in elderly total knee arthroplasty patients.
A 3D"Sandwich" Co-culture System with hPPSCs and hUVECs Supports Mouse Embryo Development from E3.5 to E7.5 In Vitro
Authors: Teng Yili , Zhang Linye , Ye Zihui , Chang Binwen , Tu Zheng , Du Xuguang , Wen Xi , XU JUNJUN 
Published: May 2023

Abstract
Background A variety of methods for ex-utero culture systems have been explored, but there remain limitations in terms of in vitro culture platforms used prior to the implantation of mouse embryos, and the ability of mouse blastocysts to develop normally in vitro has not been established. Whether vascular niche support mouse embryo development from embryonic day (E) 3.5 to E7.5 in vitro is unknown.Methods We established a three-dimensional (3D) “sandwich” vascular-niche culture system with in vitro culture medium (IVCM) with human placenta perivascular stem cells (hPPSCs) and human umbilical vein endothelial cells (hUVECs), as supportive cells, which were seeded into the bottom layer of Matrigel to test mouse embryos from E3.5 to E7.5 in vitro. Mouse embryos from E3.5 to E7.5 development rates and greatest diameters at each stage were quantitatively determinated using statistics of SPSS software. Pluripotent markers and embryo transplantation in vivo were used to monitor mouse embryo quality and function.Results Embryos in the IVCM + Cells (hPPSCs + hUVECs) showed higher development rates and greatest diameters at each stage than those in the IVCM group. Embryos in the IVCM + Cells group cultured to E5.5 resembled natural egg cylinders in morphology and expressed specific embryonic cell markers, including Oct4 and Nanog, which were features similar to embryos developed in vivo. After transplantation, the embryos could be re-implanted in the internal uterus and continue to develop to a certain stage.Conclusions Therefore, the 3D in vitro culture system enabled the development of embryos from E3.5 to E7.5, and the vascularization microenvironment constructed by Matrigel, hPPSCs, and hUVECs significantly promoted the development of implanted embryos. This system allowed us to further study the physical and molecular mechanisms of embryo implantation in vitro.
Aminophylline targets miR-128-3p/Slc7a11 axis to attenuate neuronal ferroptosis after traumatic brain injury
Authors: Xu Yang , Liu Jinyuan , Sun Yuwen , Yang Qiuyun , Li Manrui , Liao Miao , Lv Meili , Liang Weibo , Chen Xiameng 
Published: May 2023

Abstract
Traumatic brain injury (TBI) is a significant global health issue due to its high morbidity and mortality, as well as its adverse outcomes. The resulting excessive healthcare and economic burden make it urgent to find effective drugs for TBI. Aminophylline (AMP), a classical drug widely used in the treatment of cardiovascular diseases and bronchiectasis, has recently been found to play an important role in protecting neurons in hypoxia brain injury by a variety of mechanisms. However, its therapeutic effect on TBI remains unknown. To this end, the present study aims to explore whether and how AMP improves TBI prognosis, as well as the underlying mechanisms. We found that AMP administration ameliorated neuronal ferroptosis in the cortex, improved cognitive impairment and motor dysfunction in mice following controlled cortical impact (CCI). microRNA (miRNA) profiling and related validation experiments revealed that miR-128-3p expression significantly decreased at 3h and 6h after CCI, and this change was further enhanced after AMP administration. Subsequent gain of function studies demonstrated that over-expressing miR-128-3p can aggravate neuronal ferroptosis by targeting recombinant solute carrier family 7, member 11 (Slc7a11), through which AMP exerted its therapeutic role in attenuating the cognitive impairment and motor dysfunction in mice following CCI. Overall, this study reports for the first time that AMP protects against ferroptotic neuronal death in CCI mice through miR-128-3p / Slc7a11 axis, which could be a potential treatment for TBI patients.
Groundwater level modeling using multi-objective optimization hybridizing artificial intelligence
Authors: Banadkooki Fatemeh Barzegari , Haghighi Ali Torabi 
Published: May 2023

Abstract
Estimating groundwater level (GWL) fluctuations is essential for integrated water resource management in arid and semi-arid regions. This study promotes the multi-layer perceptron (MLP) learning process using hybrid evolutionary algorithms. This hybrid metaheuristic algorithm was applied to overcome MLP difficulties in the learning process, including its low conversions and local minimum. Also, the hybrid model benefits from the advantages of two objective function procedures in finding MLP parameters that result in a robust model regardless of over and under-estimating problems. These algorithms include none dominated sorting genetic algorithm (NSGA II) and multi-objective particle swarm optimisation (MOPSO) in different patterns, including MLP–NSGA-II, MLP–MOPSO, MLP-MOPSO–NSGA-II, and MLP-2NSGA-II–MOPSO. Temperature, precipitation and GWL datasets were used in various combinations and delays as model input candidates. Finally, the best model inputs were selected using the correlation coefficient (R2). Input parameters include temperature and precipitation delays of 3, 6, and 9 months and GWL delays of 1 to 12 months. In the next step, the performance of the different combinations of MLP and hybrid evolutionary algorithms was evaluated using The root mean square error (RMSE), correlation coefficient (R), and mean absolute error (MAE) indices. The outcomes of these evaluations revealed that the MLP-2NSGA-II-MOPSO model, with an RMSE=0.073, R=0.98, and MAE=0.059, outperforms other models in estimating GWL fluctuations. The selected model benefits from the advantages of both MOPSO and NSGA-II regarding accuracy and speed. The results also indicated the superiority of multi-objective optimization algorithms in promoting MLP performance.
Molecular Epidemiological and Genetic Characterization of Pseudorabies Virus in Guangxi, China
Authors: Huang Xiangmei , Qin Shuying , Wang Xuying , Xu Lishi , Zhao Shuo , Ren Tongwei , Ouyang Kang , Chen Ying , Wei Zuzhang , Qin Yifeng , Huang Weijian 
Published: May 2023

Abstract
Pseudorabies virus (PRV) is an important pathogen that can cause harm to the pig population. However, there have been a number of large-scale outbreaks of pseudorabies on Chinese farms where animals have been previously vaccinated with the Bartha-K61 vaccine since 2011. In order to understand the epidemiological trend and genetic variations of PRV in Guangxi province, China, 819 tissue samples were collected from swine farms suspected of PRV infection from 2013–2019. In this study, these were tested for infectious wild strains of PRV. The results showed a positive rate of PRV in Guangxi province of 28.21% (231/819). 36 wild-type PRV strains were successfully isolated from the PRV-positive tissue samples, and a genetic evolutionary analysis was performed based on the gB, gC, gD, gE and TK genes. 30 of the PRV strains were clustered with the Chinese variant strains, HeN1-China-2012 and HLJ8-China-2013. In addition, 5 PRV strains were genetically related to the Chinese classical strains, and one isolate was a recombinant strain of the PRV variant and the vaccine strain, Bartha-K61. Amino acid sequence analysis showed that all 36 PRV strains had characteristic variant sites in the amino acid sequences of the gB, gC, gD and gE proteins. Pathogenicity analysis showed that, compared to the PRV classical strains, the PRV variant strains had a stronger pathogenicity in mice together with a lower LD50. Taken together, our results showed that wild-type PRV infections are common in pig farms in the Guangxi province of China, and that the dominant prevalent strains were those of the PRV variants. The PRV variant strains also had a stronger pathogenicity in mice. Our data will provide a useful reference for understanding the prevalence and genetic evolution of PRV in China.
Evaluation of nurses' knowledge and performance regarding preparation and injection of intravenous drugs in pediatric wards in Iran
Authors: Shahzeydi Amir , kallhor Faramarz , Khaksar Sajjad , Sabzghabaee Ali Mohammad , Joonbakhsh Fatemeh , Ajoodaniyan Najmeh 
Published: May 2023

Abstract
Background A error in intravenous injection in pediatric wards can cause irreparable injuries. This study aimed to determine the level of knowledge and performance of nurses in terms of preparation and injection of intravenous drugs in pediatric wards of hospitals affiliated to Isfahan University of Medical Sciences.Methods This cross-sectional study was conducted in 2022 on 156 nurses working in pediatric wards. The data was collected with demographic information questionnaire and the knowledge and performance of the participants were determined using a researcher-made questionnaire, including the five rights of medication administration (preparation and injection, medication error, drug side effects, family empowerment, and documentation) using self-reporting and observation methods. Formal and content validity was calculated using the opinions of 10 experts and Cronbach's alpha with 40 samples.Results The mean and standard deviation of total nurses' knowledge and performance scores were 58.31 + 10.1 and 66.1 + 14.4, respectively. Moreover, the mean and standard deviation of nurses' knowledge scores were 63.55 + 14.3 for documentation, 46.1 + 7.9 for preparation and injection, 73.9 + 12.3 for drug side effects, 58.4 + 10.2 for medication error, and 69.4 + 9.4 for family empowerment. Besides, the mean performance was 69.1 + 17.6 for documentation, 61.3 ± 9.9 for preparation and injection, 78.21 + 12 for drug side effects, 58.6 + 15 for medication error, and 65.4 + 17.7 for family empowerment.Conclusion The results showed that the mean knowledge and pharmacological performance of nurses working in pediatric wards in different areas of the principles of medicine were not at the desired level, and this can affect children adversely.
Influencing Factors of Post-traumatic Stress Disorder among Operating Room Nurses in Large Comprehensive Hospitals
Authors: Ge Yu-Xin , Sun Xin-Xing , Lin Chen , Yu Li , Zhang Jun 
Published: May 2023

AbstractThis is an investigation of the current situation of post-traumatic stress disorder (PTSD) among operating room nurses in large comprehensive hospitals and provide a scientific basis for managers to formulate effective intervention measures. In total, 266 operating room nurses were randomly selected from 6 large comprehensive hospitals in Zhejiang Province via a multi-stage random sampling method. The self-designed general information questionnaire and Chinese version of the PTSD self-rating scale (PCL-C) were used for the investigation. According to the PCL-C scores, the nurses were divided into two groups: ≥ 41 was divided into a PTSD group and < 41 was divided into a non-PTSD group. A single factor comparison was performed on the general data line and then a multivariate logistic analysis was conducted. The total score of the PCL-C was 45.41 ± 9.46 (95%CI 44.27–46.55), including an avoidance numbness symptom score of 19.13 ± 4.65 (95%CI 18.57–19.69), a re-experience symptom score of 12.82 ± 3.37 (95%CI 12.41–13.23), and a high vigilance symptom score of 13.46 ± 3.52 (95%CI 13.03–13.88). A univariate and multivariate logistic analysis showed there were significant differences in age, marital status, working years, professional titles, positions and shift arrangements between the two groups (P < 0.05). The PTSD incidence among operating room nurses in large comprehensive hospitals is relatively high. Factors such as age, marital status, working years, professional title, position, and shift scheduling significantly impacted their PTSD incidence. Therefore, managers can formulate effective intervention measures based on these results to reduce the incidence of PTSD among operating room nurses.