bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the difference in Diagnostic accuracy of MRI between ccRCC and pRCC :- A meta Analysis
Authors: Desai Dev , Majdouli Sanae , Raval Dwija , Andharia Dev , Shah Abhijay B. , Shah Hetvi 
Published: June 2023

AbstractBackgroundRenal cell carcinoma (RCC) comprises cancer originating from the renal epithelium and takes up for >90% of cancers in the kidney. The disease consists of >10 histological and molecular subtypes, of which clear cell RCC (ccRCC) is the most common and is responsible for most cancer-related deaths. In an attempt to ensure an early diagnosis to plan the further course of surgical intervention, pre-operative diagnosis plays an important role. MRI plays a crucial role in the diagnosis of RCC and planning for surgery for presumed RCC, especially for identifying enhanced soft tissue within renal lesions. The purpose of this meta-analysis is to ascertain the accuracy of MRI in diagnosing papillary renal cell carcinoma (pRCC) compared to clear cell carcinoma to reach a definitive diagnosis and thus, help in surgical intervention.MethodsMedical literature was comprehensively searched and reviewed without restrictions to particular study designs, or publication dates using PubMed, Cochrane Library, and Google Scholar databases for all relevant literature. The extraction of necessary data proceeded after specific inclusion and exclusion criteria were applied. In this Meta-Analysis, a total of 5 papers involving 755 lesions were considered for Clear cell carcinoma. A total number of 13 papers regarding 1009 lesions of papillary renal cell carcinoma were considered. wherein two writers independently assessed the caliber of each study as well as the use of the Cochrane tool for bias risk apprehension. The statistical software packages RevMan (Review Manager, version 5.3), SPSS (Statistical Package for the Social Sciences, version 20), and Excel in Stata 14 were used to perform the statistical analyses.ResultsWe calculated the sensitivity and specificity of MRI in diagnosing pRCC and ccRCC in the different papers, For the MRI in ccRCC, The sensitivity is 0.81 with a CI of 95% in a range of 0.77 to 0.86, the mean being 0.049. The Specificity of the MRI is 0.77 with a CI of 95% in a range of 0.68 to 0.86, the mean being 0.091. For the pRCC, The Sensitivity of the MRI in pRCC is 0.66 with a CI of 95% in a range of 0.52 to 0.80; the mean being (0.14). The Specificity of the MRI in pRCC is 0.87 with a CI of 95% in a range of 0.80 to 0.94, the mean being 0.072.ConclusionMagnetic resonance imaging (MRI) offers a thorough evaluation of renal masses that takes in to account both, functional factors and several types of tissue contrast. In light of the above mentioned clinical requirements, these characteristics of MRI have sped up the process of early detection, diagnosis, staging, and evaluation of the aggressiveness and therapeutic r esponse of RCC
Chromosome-scale Genome Assembly of the AllopolyploidArundo donax
Authors: Ren Mengmeng , Liu Fupeng , Han Xiaohong , Wu Daohong , Peng Hai 
Published: June 2023

AbstractArundo donaxL (A. donax). is a promising energy crop with high biomass and wide adaptability, while lack of reference genome limiting the genetic improvement of this crop. Here, we report two different chromosome-scale assemblies ofA. donaxgenome (0004 and 0408) using Pacbio SMRT sequencing and Hi-C technology. The genome size of these two assemblies is 1.30 Gb / 2.86 Gb with contig N50 33.15 Mb / 34.62 Mb respectively. A total of 74,403 / 167,586 gene models were predicted in 0004 and 0408 genome, of which over 90% of genes were functionally annotated. Comparative genome analysis revealed that 0004 is alloenneaploid and 0408 is allohexaploid. Further analysis revealed thatA. donaxundergone strong gene family expansion and two whole-genome duplication events during evolution. Our results will enhance genetic understanding and promote the genetic improvement ofA. donax.
Enzyme-mediated alkynylation enables transcriptome-wide identification of pseudouridine modifications
Authors: Wang Yuru , Zhang Lisheng , Zhang Wen , Gao Boyang , Ye Chang , Dai Qing , Wang Ke , Luo Minkui , Pan Tao , He Chuan 
Published: June 2023

AbstractPseudouridine (Ψ) is one of the most abundant chemical modifications that exists in various types of RNA species and is known to play important roles in RNA function. The advances in studies of Ψ in less abundant messenger RNA species have been hindered by a lack of suitable methods to precisely and sensitively map their distributions. Here we show that a methyltransferase fromMethanocaldococcus jannaschiican label RNA Ψ efficiently and specifically with various functional groups, both in isolated RNA and inside cells. We leveraged this enzymatic labeling strategy to develop ELAP-seq as a facile method to enrich Ψ-modified transcripts for the detection of Ψ modifications at single base resolution with high sensitivity and low background. Using this method, we identified over 10, 000 candidate Ψ sites from human transcripts, which provides new insights into Ψ biosynthesis and function. Our study provides a chemical biology method that specifically labels Ψ for its detection and functional alteration.
Bio-based decontamination and detoxification of total petroleum hydrocarbon contaminated dredged sediments: perspectives to produce constructed technosols in the frame of the circular economy
Authors: Becarelli Simone , Bernabei Giacomo , Siracusa Giovanna , Baderna Diego , Castiglione Monica Ruffini , Simone Giampiero De , Gregorio Simona Di 
Published: June 2023

AbstractTo accelerate the depletion of total petroleum hydrocarbons, a hydrocarburoclastic ascomycetes,Lambertellasp. MUT 5852, was bioaugmented to dredged sediments co-composting with a lignocellulosic matrix. After only 28 days of incubation, a complete depletion of the contamination was observed. The 16S rDNA metabarcoding of the bacterial community and a predictive functional metagenomic analysis was adopted to evaluate potential bacterial degrading and detoxifying functions. A combination of toxicological assays on two eukaryotic models, the root tips ofVicia fabaand the human intestinal epithelial Caco-2 cells, was adopted to assess the robustness of the process not only for the decontamination but also for the detoxification of the dredged sediments. Bacterial taxa, such asKocuriaandSphingobacteriumsps. resulted to be involved in both the decontamination and detoxification of the co-composting dredged sediments by potential activation of diverse oxidative processes. At the same time, theKocuriasp. showed plant growth promoting activity by the potential expression of the 1-aminocyclopropane-1-carboxylate deaminase activity, providing functional traits of interest for a technosol in terms of sustaining primary producer growth and development.
Cortical tension regulates Hippo signaling via Par-1-mediated Kibra degradation
Authors: Tokamov Sherzod A. , Buiter Stephan , Ullyot Anne , Scepanovic Gordana , Williams Audrey Miller , Fernandez-Gonzalez Rodrigo , Horne-Badovinac Sally , Fehon Richard G. 
Published: June 2023

AbstractThe Hippo pathway is an evolutionarily conserved regulator of tissue growth. Multiple Hippo signaling components are regulated via proteolytic degradation. However, how these degradation mechanisms are themselves modulated remains unexplored. Kibra is a key upstream pathway activator that promotes its own ubiquitin-mediated degradation upon assembling a Hippo signaling complex. Here, we demonstrate that Hippo complex-dependent Kibra degradation is modulated by cortical tension. Using classical genetic, osmotic, and pharmacological manipulations of myosin activity and cortical tension, we show that increasing cortical tension leads to Kibra degradation, whereas decreasing cortical tension increases Kibra abundance. Our study also implicates Par-1 in regulating Kib abundance downstream of cortical tension. We demonstrate that Par-1 promotes ubiquitin-mediated Kib degradation in a Hippo complex-dependent manner and is required for tension-induced Kib degradation. Collectively, our results reveal a previously unknown molecular mechanism by which cortical tension affects Hippo signaling and provide novel insights into the role of mechanical forces in growth control.
GTPase activating protein DLC1 spatio-temporally regulates Rho signaling
Authors: Heydasch Max , Hinderling Lucien , van Unen Jakobus , Dobrzynski Maciej , Pertz Olivier 
Published: June 2023

AbstractTightly regulated spatio-temporal Rho GTPase activity patterns regulate morphogenetic processes such as cell migration. Emerging evidence suggests that binding of Guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs) to the cytoskeleton or adhesions mediate feedback regulation to spatio-temporal Rho GTPase activation. To explore such feedback regulation, we study the Rho specific GAP Deleted in Liver Cancer 1 (DLC1) which binds to focal adhesions (FAs) through mechanosensitive interactions. Using a FRET biosensor, we show that DLC1 loss of function leads to global increase in Rho activity and contractility throughout the cell without affecting a striking lamellar RhoA activity band in fibroblasts. To interrogate the Rho GTPase signaling flux, we build a genetic circuit consisting of an optogenetic actuator to control Rho activity, and a Rho activity biosensor. In spreading cells at steady state, optogenetic manipulation of Rho activity reveals that DLC1 controls the rate of Rho activation rather than duration, both at FAs and at the plasma membrane (PM). Local and reversible optogenetic control of contractility shows that DLC1 associates/dissociates with FAs during their reinforcement/relaxation. This might provide positive feedback that locally increases the rate of Rho activation at FAs that experience local tension to facilitate FA disassembly. Our results indicate that DLC1 operates both at the PM and at FAs to regulate global Rho activity levels at steady state, or to amplify local Rho activity at FAs experiencing a strong mechanical input, presumably to induce robust FA disassembly. This provides new insights in the complexity of spatio-temporal Rho GTPase signaling.
Proteomics reveals how the tardigrade damage suppressor protein teaches transfected human cells to survive UV-C stress
Authors: Shaba Enxhi , Landi Claudia , Marzocchi Carlotta , Vantaggiato Lorenza , Bini Luca , Ricci Claudia , Cantara Silvia 
Published: June 2023

AbstractThe genome sequencing of the tardigradeRamazzottius varieornatusrevealed a unique nucleosome-binding protein, named Damage Suppressor (Dsup), which resulted to be crucial for the extraordinary abilities of tardigrades in surviving extreme stresses, such as UV. Evidence in Dsup transfected human cells, suggests that Dsup mediates an overall response of DNA damage signaling, DNA repair and cell cycle regulation resulting in an acquired resistance to stress. Given these promising outcomes, our study attempts to provide a wider comprehension of the molecular mechanisms modulated by Dsup in human cells, and to explore the Dsup-activated molecular pathways under stress. We performed a differential proteomic analysis of Dsup-transfected and control human cells, under basal condition and at 24-hour recovery after exposure to UV-C. We demonstrate by enrichment and network analyses, for the first time, that even in the absence of external stimuli and more significantly after stress, Dsup activates mechanisms involved with the Unfolded Protein Response, the mRNA processing and stability, cytoplasmic stress granules, the DNA Damage Response and the telomere maintenance. In conclusion, our results shed new light on Dsup-mediated protective mechanisms, and increase our knowledge of the molecular machineries of extraordinary protection against UV-C stress.
The role of social media content on value co-creation in the food sector: a strategic relationship perspective
Authors: Gokce Aytac , Bazi Saleh , Safavi Bijan , Georgiadou Elena , Hajli Nick 
Published: June 2023

PurposeCustomers' participation in the online health community to create value with the brand is growing research interest. In addition, customers are using social media platforms to create value in the food sector. This rises points to the need to study consumers' interactions with online communities and the role of social media content and customer satisfaction in such an environment.Design/methodology/approachThis research collects data using a survey approach. The data were analysed using a partial least square-structural equation modelling (PLS-SEM).FindingsThe findings indicated the impact of social media content and satisfaction on value co-creation in healthy food online communities. The study’s results provide significant new insights into the food sector during the pandemic.Originality/valueThis research enhances the knowledge of satisfaction and value co-creation in the social media context. The findings build on the previous literature on value co-creation, add to the food sector and explain the mediating role of satisfaction between social media content and value co-creation.
Colorful candy, teen vibes and cool memes: prevalence and content of Instagram posts featuring ultra-processed products targeted at adolescents
Authors: Ares Gaston , Alcaire Florencia , Gugliucci Vanessa , Machín Leandro , de León Carolina , Natero Virginia , Otterbring Tobias 
Published: June 2023

Purpose
The current research aimed to examine the prevalence of Instagram posts featuring ultra-processed products targeted at adolescents in Uruguay and hence investigate the frequency of such posts among a vulnerable consumer segment in a country that cannot be classified as WEIRD (i.e. Western, educated, industrialized, rich and democratic).

Design/methodology/approach
The study relied on a cross-sectional content analysis. A total of 2,014 Instagram posts promoting ultraprocessed products or brands commercializing such products, generated by 118 Instagram accounts between August 15th, 2020, and February 15th, 2021, were analyzed. Nine indicators of food marketing targeted at adolescents were selected to identify posts targeted at this age segment. Inductive coding was used to describe the content of the posts. Descriptive statistics and generalized linear models were used to analyze the data.

Findings
In total, 17.6% of the posts were identified as targeted at adolescents. Graphic design and adolescent language were the most prevalent indicators of marketing targeted at adolescents, followed by explicit references to adolescents or young adults and memes. Posts identified as targeted at adolescents mainly promoted snacks and discretionary foods. Differences in the content of posts identified as targeted and not targeted at adolescents were observed.

Research limitations/implications
The analysis was restricted to one social media platform in one country during a limited period of time, which limits the generalizability of the findings to other media platforms, samples and settings.

Social implications
Results stress the need to implement digital food marketing regulations to reduce exposure of adolescents to the deleterious effects of stemming from marketing of unhealthy foods and provide empirical evidence to inform their development.

Originality/value
The study breaks new ground by analyzing the prevalence and exploring the characteristics and content of Instagram posts promoting ultra-processed products to adolescents in an under-researched geographic area of the world.
Sustainability of renewable energy production: empirical evidence from developing and middle-income countries
Authors: Osei Bismark , Fiagbe Agbemavor Korsi , Kulu Evans 
Published: June 2023

Purpose
This study aims to examine the appropriate measures needed toward achieving sustainability of renewable energy production among developing and middle-income countries.

Design/methodology/approach
This study uses semi-annual panel data covering the period 2000–2020 among 152 developing and middle-income countries and Cox proportional hazard model for the analysis.

Findings
Estimates indicate that effective operations of environmental institutions, investment in research and development, subsidizing the production of renewable energy, government investment in producing renewable energy and investment in renewable energy production made by the private sector will contribute immensely toward achieving sustainability of renewable energy production.

Practical implications
This study recommends that governments should rationalize their expenditures to mobilize enough resources for investment in renewable energy production. Again, operations of environmental institutions should be enhanced through giving their managers’ performance contracts and licensing its employees. Enabling environment should be created for private sector to increase their investment in renewable energy production.

Originality/value
Empirical studies have been carried out exploring measures to deal with climate change. Nonetheless, the appropriate measures needed toward achieving sustainability of renewable energy production among developing and middle-income countries have not been explored in existing empirical studies. Hence, this study fills the gap in existing empirical studies.
Three decades of house purchase decisions: a scoping review and thematic classification
Authors: Abhang Janhavi , Ravi Kumar V.V. 
Published: June 2023

Purpose
This study aims to develop a database of existing academic information in house purchase decision (HPD) using systematic literature review (SLR), to facilitate worldwide advancement of research under HPD domain.

Design/methodology/approach
This research examined papers from two reputable databases – Scopus and Google Scholar – from 1992 to 2022 using a scoping review technique (Arksey and O’Malley, 2005) and a theme analysis method. Out of 374, 181 articles fit the inclusion parameters and were evaluated using the theme analysis approach.

Findings
Data from 181 articles was evaluated thematically to create a thematic map of HPD research. Five main themes and their sub-themes were identified: consumer behaviour, housing attributes, factors influencing purchasing decisions, investment analysis and demographics, which proved essential in understanding HPD and customer preferences for house purchase.

Practical implications
Data from 181 articles were evaluated thematically to create a thematic map of HPD research. This SLR intends to provide useful new insights on consumer concerns about home purchases in the rapidly developing residential real estate market and the issues that marketers, housing sector stakeholders, real estate industry and existing and future researchers should prioritize.

Originality/value
This research is unique such that it is the only 30-year-long SLR on the subject matter of HPD. This paper makes a significant contribution to residential real estate domain signifying the present state of research in HPD.
Competitive peer influence on knowledge contribution behaviors in online Q&A communities: a social comparison perspective
Authors: Shi Chencheng , Hu Ping , Fan Weiguo , Qiu Liangfei 
Published: June 2023

PurposeUsers' knowledge contribution behaviors are critical for online Q&A communities to thrive. Well-organized question threads in online Q&A communities enable users to clearly read existing answers and their evaluations before contributing. Based on the social comparison and peer influence literature, the authors examine peer influence on the informativeness of knowledge contributions in competitive settings. The authors also consider three levels of moderating factors concerning individuals' perception of competitiveness: question level, thread level and contributor level.Design/methodology/approachThe authors collected data from one of the largest online Q&A communities in China. The hypotheses were validated using hierarchical linear models with cross-classified random effects. The generalized propensity score weighting method was employed for the robustness check.FindingsThe authors demonstrate the peer influence due to social comparison concerns among knowledge contribution behaviors in the same question thread. If more prior knowledge contributors choose to contribute long answers in the question thread, the subsequent contributions are more informative. This peer influence is stronger for factual questions and questions with higher popularity of answering but weaker in recommendation-type and well-answered questions and for contributors with higher social status.Originality/valueThis research provides a new cue of peer influence on online UGC contributions in competitive settings initiated by social comparison concerns. Additionally, the authors identify three levels of moderating factors (question level, thread level and contributor level) that are specific to online Q&A settings and are related to a contributor's perception of competitiveness, which affect the direct effect of peer influence on knowledge contributions. Rather than focus on motivation and quality evaluation, the authors concentrate on the specific content of online knowledge contributions. Peer influence here is not based on an actual acquaintance or a following relationship but on answering the same question. The authors also illustrate the competitive peer influence in subjective and personalized behaviors in online UGC communities.
The role of implicit theories in consumer intertemporal preference
Authors: Shirai Miyuri 
Published: June 2023

Purpose
This study aims to investigate the psychological process of intertemporal choices between larger-later and smaller-sooner monetary options. Prior research showed consumer impatience – a tendency to prefer a smaller-sooner option over a larger-later option. This research identifies an individual difference that predicts patience and mediators that explain the underlying mechanism.

Design/methodology/approach
Two studies are conducted. Study 1 examines whether the implicit theory of intelligence consumers endorse (i.e. entity theory vs incremental theory) constitutes an antecedent of patience and whether their thoughts regarding anticipated purchase with the chosen monetary option (i.e. hedonic versus utilitarian purchase) mediate the relationship. Study 2 analyzes whether psychological reactance toward larger-later options is a mediator in this relationship using a perceived threat to freedom and affect as reactance indicators.

Findings
Entity-oriented consumers exhibited less patience than incremental-oriented consumers, especially when anticipating a hedonic purchase. Moreover, entity-oriented consumers perceived a threat to freedom from larger-later options more strongly – this enhanced perception influenced patience through two routes. One route is that the perceived threat to freedom leads to more consideration of a hedonic purchase rather than a utilitarian purchase, thereby decreasing patience. The other route is that the perceived threat to freedom elicits a stronger negative affect, resulting in lower patience.

Originality/value
Findings of this research shed light on the understanding of patience. They demonstrate that consumers’ implicit theory orientation is a crucial individual difference that can explain patience. Also, demonstrating the mediating roles of anticipated purchase using the hedonic/utilitarian classification and psychological reactance expanded literature by showing how they internally interact.