bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infant respiratory infections modulate lymphocyte homing to breast milk
Authors: Zheng Yingying , Corrêa-Silva Simone , Rodrigues Regina Maria , Corrêa de Souza Eloisa , Macaferri da Fonseca Fernanda A. , Gilio Alfredo Elias , Carneiro-Sampaio Magda , Palmeira Patricia 
Published: January 2025

IntroductionChemokines and their receptors are essential for leukocyte migration to several tissues, including human milk. Here, we evaluated the homing of T and B lymphocyte subsets to breast milk in response to ongoing respiratory infections in the nursing infant.MethodsBlood and mature milk were collected from healthy mothers of nurslings with respiratory infections (Group I) and from healthy mothers of healthy nurslings (Group C). Total lymphocyte, T and B cells, their subset numbers, and the expression of the homing receptors CCR5, CCR6, CCR10, and CXCR3 in these cells were evaluated in milk. Maternal serum and milk chemokine, cytokine, and IgA and IgG antibody levels were also quantified.ResultsAll milk lymphocyte numbers were greater in Group I than in Group C. All CD4 T-cell subsets expressing CCR5, CCR6, and CXCR3 were higher in Group I. Within the CD8 T-cell subsets, only CCR6 and CXCR3 were higher in Group I, while CCR5 expression was higher in Group I exclusively for activated CD8 T cells. Group I showed greater numbers of all CCR6+ B-cell subsets and CXCR3+ naive B cells and plasma cells than did Group C. Infection of the nurslings promoted increased CCL20, CXCL10, IL-6, IL-8, total IgA, and IgG levels in the milk.ConclusionRespiratory infections in nursing infants stimulate an increase in cytokines and chemokines in breast milk, facilitating the recruitment and activation of lymphocytes. This process may promote immunological tolerance and help in the maturation of the infant's immune system, providing an additional strategy for passive maternal-infant protection.
ScRNA-seq unveils the functional characteristics of glioma-associated macrophages and the regulatory effects of chlorogenic acid on the immune microenvironment—a study based on mouse models and clinical practice
Authors: Wang Jiachen , Li Shenglan , Chen Yuxiao , Chen Jinyi , Wang Can , Kang Zhuang , Huang Mengqian , Cai Zehao , Fan Yuxiang , Lan Yanjie , Yu Yumeng , Bai Ruijing , Chen Feng , Jiang Jiandong , Li Wenbin 
Published: January 2025

IntroductionGlioma is the most common primary malignant brain tumor. Despite advances in surgical techniques and treatment regimens, the therapeutic effects of glioma remain unsatisfactory. Immunotherapy has brought new hope to glioma patients, but its therapeutic outcomes are limited by the immunosuppressive nature of the tumor microenvironment (TME). This study aimed to reveal the subpopulations and functional characteristics of tumor-associated macrophages (TAMs) and explore the regulatory effects of chlorogenic acid (CHA) on the immune microenvironment, as well as its potential for clinical application.MethodsIn this study, CHA was used in model mice. ScRNA - seq analysis was conducted to elucidate the differentiation trajectories and functional characteristics of bone marrow - derived monomacrophages (BMDMs) and microglia. A PPI and molecular docking model were constructed using the target prediction database. A case of a patient treated with CHA was reviewed.ResultsCHA slowed tumor growth in model mice and extended the survival time of mice. It enhanced the antigen - presenting function of macrophages and T - cell immune activation - related gene expression, activated microglia through the JAK - STAT pathway, and improved the antitumor functions. The good affinity of CHA with STAT1 was confirmed. The patient treated with CHA survived for 5 years and 6 months, achieved partial remission (PR) after 9 months of treatment, and remained alive without any new symptoms or toxic side effects. Our study revealed the subtypes and differentiation trajectories of TAMs. CHA significantly improved the immune microenvironment of glioma by modulating the function of BMDMs and microglia.DiscussionThis study may provide new insights into targeting the regulation of TME and offer theoretical and practical support for the clinical application of CHA. The results demonstrated the potential of CHA in improving the immune microenvironment and antitumor effects, which could have implications for future glioma treatment strategies.
Prognosis prediction of α-FAtE score for locoregional immunotherapy in hepatocellular carcinoma
Authors: Zheng Zehao , Guan Renguo , Zhao Rongce , Gan Junyu , Xiong Xinhao , Zou Jing-wen , Li Shaohua , Wang Qiaoxuan , Wei Wei , Mei Jie , Guo Rongping 
Published: January 2025

PurposeThe α-FAtE score, composed of alpha-fetoprotein, alkaline phosphatase, and eosinophil levels, has been reported as a predictor of prognosis in hepatocellular carcinoma (HCC) patients treated with atezolizumab plus bevacizumab. This study aimed to investigate the predictive ability of α-FAtE score for the efficacy and safety of locoregional immunotherapy as the treatment of HCC patients.Methods and patientsWe conducted a retrospective study of 446 HCC patients at Sun Yat-sen University Cancer Center from January 1st 2019 to January 1st 2023. The predictive performance was evaluated by the concordance index, the area under the receiver operating characteristics curve, the Kaplan-Meier curve and multiple Cox regression analysis.Results446 patients were divided into the α-FAtE 0-1 group (n=211) and α-FAtE 2-3 group (n=235). The median progression-free survival(PFS) of the α-FAtE 0-1 group and 2-3 group was 7.3 months (95%CI 6.6-8.7 months), and 12.3 months (95% CI 10.4–14.1 months; P&lt;0.001), respectively. The median overall survival (OS) of the α-FAtE 0-1 group and 2-3 group was 16.3 months (95%CI 13.7-21.5 months) and 34.1 months (95% CI 27.6–NA months; P&lt;0.001), respectively. HCC patients in the α-FAtE 2-3 group had higher complete response (CR) rate and experienced less drug-related adverse events than those in the α-FAtE 0-1 group. Moreover, a lower α-FAtE score was identified as an independent prognostic indicator for both OS and PFS of advanced HCC patients receiving locoregional immunotherapy.ConclusionThe α-FAtE score is a superior predictor of prognosis in HCC patients receiving locoregional immunotherapy, offering a valuable tool for patient stratification and treatment planning.
Community characteristics and relationship between gut microbiota and intratumoral microbiota in hepatocellular carcinoma
Authors: Lin Huangpeng , Ma Zexian , Li Jin , Zhu Heping , Huang Xuefeng , Chen Huimin , Tu Liang , Lian Yifan , Su Yongjie 
Published: January 2025

BackgroundThe combination of local therapy with lenvatinib and programmed cell death protein-1 (PD-1) inhibitors represents an emerging treatment paradigm for unresectable hepatocellular carcinoma (uHCC). Our study sought to investigate the interrelationship between gut microbiota and intratumoral microbiota in the context of triple therapy, with a view to identifying potential biological markers.MethodsThe gut microbial community profiles of patients with primary untreated hepatocellular carcinoma (HCC) and those treated with local therapy combined with lenvatinib and PD-1 inhibitors were analyzed by 16S rRNA gene amplicon sequencing. Additionally, microbial community profiles of tumor tissues of patients with HCC and normal liver tissues were analyzed.ResultsIn our investigation, we observed that patients with HCC who received triple therapy exhibited a notable enhancement in the abundance of Actinobacteriota and a considerable decrease in Escherichia Shigella. Patients who received hepatic artery infusion chemotherapy (HAIC) in combination with levatinib and PD-1 inhibitors exhibited significantly elevated levels of Faecalibacterium prausnitzii and Bacteroides stercoris in comparison to those who received transarterial chemoembolization (TACE) in combination with levatinib and PD-1 inhibitors. Furthermore, a notable decline in microbial diversity was observed within HCC tumors in comparison to normal liver tissues. The gut and intratumoral microbiota in HCC patients exhibited a high degree of similarity to the microbes present at the phylum level.ConclusionsGut microbiota is connected to triple therapy with local therapy combined with lenvatinib and PD-1 inhibitors for HCC. These discoveries underscore the potential of utilizing gut microbiota and intratumoral microbiota as biomarkers, as well as the possibility of triple therapy in the management of HCC.
Study based on bibliometric analysis: potential research trends in fluid management for sepsis
Authors: Liu Sihan , Pei Haoting , Wang Jing , Qiao Lujun , Wang Hao 
Published: January 2025

ObjectiveTo investigate the potential and evolving trends in fluid management for patients with sepsis, utilizing a bibliometric approach.MethodsScholarly articles pertaining to fluid therapy for sepsis patients were extracted from the Web of Science (WoS) database as of June 1, 2024. The R software package, “Bibliometrix,” was utilized to scrutinize the primary bibliometric attributes and to construct a three-field plot to illustrate the relationships among institutions, nations, and keywords. The VOSviewer tool was employed for author analysis, keyword co-occurrence analysis, and data visualization. Additionally, CiteSpace was used to calculate citation bursts and keywords.ResultsA comprehensive retrieval from the Web of Science (WoS) database yielded a total of 2,569 publications. The majority of these articles were predominantly published by two countries, namely the United States (US) and China. Among the myriad of journals, Critical Care and Journal for Intensive Care Medicine emerged as the most prolific. In terms of institutional contribution, the University of California System stood out as the most productive. Recent analysis of keywords revealed a significant citation burst for terms such as “balanced crystalloids” and “critically ill children”.ConclusionThere is a growing focus on the connection between fluid management and the treatment of sepsis, with research in this area being at an advanced stage.
The effect of deep magnetic stimulation on the cardiac-brain axis post-sleep deprivation: a pilot study
Authors: Cao Qiongfang , Zhang Peng , Liu Fangfang , Jin Mengyan , Wang Yuhan , Zeng Hanrui , Weng Xiechuan , Xu Fan 
Published: January 2025

IntroductionSleep deprivation (SD) significantly disrupts the homeostasis of the cardiac-brain axis, yet the neuromodulation effects of deep magnetic stimulation (DMS), a non-invasive and safe method, remain poorly understood.MethodsSixty healthy adult males were recruited for a 36-h SD study, they were assigned to the DMS group or the control group according to their individual willing. All individuals underwent heart sound measurements and functional magnetic resonance imaging scans at the experiment’s onset and terminal points. During the recovery sleep phase, DMS was applied twice for 30 min before sleep onset and upon awakening to the individuals in the DMS group. Two-factor analysis was used to disclose the changes in two status and intervention effect in groups, along with Spearman rank correlation analysis to assess the correlation between brain activity and heart activity, the linear regression analysis was performed to explore the effect of DMS on brain regions to regulated the heart activity. Additionally, bootstrapping analysis was employed to verify the mediation effect.ResultsThe results indicated that the DMS group cardiac cycle duration was 0.81 ± 0.04 s, CON group was 0.80 ± 0.03 s, DMS presented a prolong effect (F = 0.32, p = 0.02), and all heart frequency and intensity indexes value were lower than CON group (p &lt; 0.01). Two-factor analysis demonstrated the significant differences in the left insula and orbitofrontal inferior gyrus, which DC_Weight (0.25) value were lower 0.50 (p &lt; 0.01), 0.42 (p &lt; 0.01) after DMS. Furthermore, the correlation analysis confirmed that the negative association between the left orbital inferior frontal and left insula with the heart sound index (p &lt; 0.05), such as Δ left orbital inferior frontal were negatively correlated with Δ Systolic_intensity (rho = −0.33, p &lt; 0.05), Δ Diastolic_intensity (rho = −0.41, p &lt; 0.05), Δ S1_intensity (rho = −0.36, p &lt; 0.05), and Δ S2_intensity (rho = −0.43, p &lt; 0.05). Δ Left insula was negatively correlated with Δ Diastolic_intensity (rho = −0.36, p &lt; 0.05), Δ S1_intensity (rho = −0.33, p &lt; 0.05), and Δ S2_intensity (rho = −0.36, p &lt; 0.05). Mediated effect analysis showed that DMS affected S2_intensity by intervening in brain regions.ConclusionThese findings suggest a causal effect on the cardiac-brain axis following 36 h of SD. The non-invasive intervention of DMS effectively regulates both brain and heart functions after SD, promoting homeostatic balance. The DMS can affect the cardiac-brain axis, offering a means to restore balance following extended periods of SD.
Postoperative fever after elective minimally invasive resection for gastric and colorectal cancer: incidence, risk factors and characteristics
Authors: He Fan , Tang Chenglin , Yang Fuyu , Zhao Dongqin , Xiong Junjie , Zou Yu , Chen Defei , Huang Guoquan , Qian Kun 
Published: January 2025

PurposeTo analyze the incidence and risk factors of postoperative fever (POF) in gastrointestinal cancer (GIC), discuss the influence of POF on short-term clinical outcomes, and predict anastomotic leakage (AL) based on POF characteristics.MethodsOverall, 1362 patients that underwent radical resection for GIC were retrospectively analyzed. POF was defined as a postoperative temperature ≥38°C during hospitalization. Patients were divided according to whether they experienced POF. The influence of POF on short-term clinical outcomes was analyzed using propensity score matching. A subgroup analysis was conducted to examine the relationship between different POF characteristics and AL or infection-related complications.ResultsPOF occurred in 172 patients (12.6%). Overall, 115 patients (66.9%) had fever ≥38.6°C, while 105 (61.0%) had fever at postoperative day (POD) 2, and 73 (42.4%) had POF multiple times. Multivariate analysis showed that patients with a preoperative albumin level &lt; 37 g/L (odds ratio [OR]=1.57, p=0.016), operative time &gt;195min (OR=1.55, p=0.020), and radical gastrectomy (OR=1.84, p=0.009) were more likely to develop POF. Compared to patients without fever, drainage tube indwelling time, duration of antibiotic use, and hospital stay were prolonged, while AL and infection-related complications were more common in patients with POF. POF ≥38.6°C (OR=1.74, p=0.039) and PCT &gt;0.7 ng/mL (OR=2.99, p=0.022) at POD 3 were early predictors of AL.ConclusionPOF was closely related to preoperative albumin levels, operative time, and type of operation, and it delayed postoperative recovery in patients with GIC. And POF ≥38.6°C and PCT &gt;0.7 ng/mL at POD 3 were independent predictors of AL.
External validation of radiobiological models for local control prediction in lung cancer patients treated with stereotactic body radiation therapy
Authors: Huang Bao-Tian , Lin Pei-Xian , Wang Ying , Luo Li-Mei 
Published: January 2025

BackgroundThe debate regarding the accuracy of radiobiological models for local control (LC) prediction in lung cancer patients undergoing stereotactic body radiation therapy (SBRT) remains unresolved. The study seeks to externally validate the predictive efficacy of radiobiological models using single-institutional SBRT database.MethodsThe cohort comprised 153 patients diagnosed with primary or metastatic lung cancer who underwent SBRT. The study employed three radiobiological models to estimate the probability of 2-year LC, including the Liu model, Klement model, and Ohri model. Furthermore, the likelihood of 3-year LC was predicted using the Liu model, Klement model, Gucken model, and Santiago model. The performance of the prediction models was assessed through the AUC values of the receiver operating characteristic (ROC) curve and the calibration plots.ResultsLocal recurrence was observed in 38.6% of patients (59/153) within two years, and in 43.1% (66/153) within three years after the radiotherapy. The ROC curves indicated discriminative power for all the 2-year and 3-year models, with the exception of the Klement model. The Ohri model showed a significantly improved discriminative ability than the Klement model for 2-year prediction, while it was not statistically significant when compared to the Liu model. However, no significant differences were found among the four models in terms of 3-year LC prediction. The calibration plots, using the Hosmer-Lemeshow goodness-of-fit test, confirmed that the predicted probabilities of the models were in agreement with the actual observation with P&gt;0.05, except for the 2-year LC prediction using the Klement model.ConclusionConsidering the balance between prediction accuracy and model simplicity, it is recommended to utilize the Ohri model for 2-year LC prediction and either the Gucken model or Santiago model for 3-year LC prediction.
Development of right ventricular electromechanical dyssynchrony following surgical repair of tetralogy of Fallot in infants
Authors: McCrary Andrew W. , Collins Sydney D. , Spector Zebulon Z. , Kropf P. Andrea , Barker Piers C. A. , Kisslo Joseph , Forsha Daniel E. 
Published: January 2025

BackgroundIn adolescents and adults with tetralogy of Fallot (TOF), right ventricle (RV) electromechanical dyssynchrony (EMD) due to right bundle branch block (RBBB) is associated with reduced exercise capacity and RV dysfunction. While the development of RBBB following surgical repair of tetralogy of Fallot (rTOF) is a frequent sequela, it is not known whether EMD is present in every patient immediately following rTOF. The specific timing of the onset of RBBB following rTOF therefore provides an opportunity to assess whether acute RBBB is associated with the simultaneous acquisition of EMD.MethodsTransthoracic echocardiography with speckle tracking analysis for RV global longitudinal strain (GLS) and 12-lead ECG were performed prospectively on 20 infants following rTOF. Three apical RV views were obtained using analogous imaging planes to the standard LV views to provide a comprehensive evaluation. Regional RV GLS patterns were categorized as synchronous, EMD, or indeterminate. EMD was defined as an early-terminated septal contraction opposed by early stretch and post-systolic peak contraction in the activation delayed RV free wall. An indeterminate pattern was defined as a lack of fully synchronous contraction of all segments but not meeting criteria for EMD. Pre-rTOF echocardiograms and ECGs were analyzed to confirm the presence of synchronous contraction and a normal QRS pattern and duration prior to surgery.ResultsTwenty TOF infants (median age 87 days; 8 days from surgery to post-rTOF evaluation) demonstrated QRSd prolongation following rTOF (pre-rTOF 58 ± 9 ms; post-rTOF 97 ± 14 ms; p &lt; 0.001) with new RBBB morphology in all but one patient. All pre-rTOF RV strain patterns were synchronous. Post-rTOF RV strain analysis showed EMD in 25% (5/20) and an indeterminate pattern in 40% (8/20) with the remaining 35% (7/20) maintaining a synchronous pattern, including the patient without RBBB. The EMD group had the lowest RV GLS following repair (p = 0.006).DiscussionAcquisition of acute QRS prolongation in a RBBB pattern is near-universal following rTOF but without matched or identical patterns of dyssynchrony, suggesting that variations in the time from electrical to electromechanical dyssynchrony potentially caused by differences in right bundle branch anatomy and injury may be relevant to electromechanical outcomes.
Dribble deficit as an effective measure of dribbling ability independent of sprinting performance in professional female handball players
Authors: Pavlović Ljubomir , Lazić Anja , Čović Nedim , Pišot Rado , Petronijević Milan , Milanović Zoran 
Published: January 2025

PurposeThe purpose of this study was to determine the relationship between linear and change-of-direction sprinting performance with dribbling performance and Dribble Deficit in professional female handball players.MethodsEleven professional female handball players (mean age: 21.12 ± 4.34 years; body height: 171.59 ± 4.52 cm; body weight: 66.29 ± 5.73 kg) participated in the study. Each participant completed several linear (sprint over 10, 20, and 30 m) and change-of-direction tests (slalom test, zig-zag test, 505 test), first without the ball (sprinting performance) followed by ball dribbling (dribbling performance). Dribble Deficit was calculated indirectly as the time difference between the best trial while dribbling minus the best trial without dribbling.ResultsA large to very large correlation was observed between the linear sprint and dribbling performance (r = 0.53–0.78), as well as between change-of-direction sprinting performance and dribbling performance (r = 0.66–0.88). The study also showed a moderate to perfect relationship between linear dribbling performance and Dribble Deficit (r = 0.46–0.93), and a large relationship between change-of-direction dribbling performance and Dribble Deficit (r = 0.54–0.55), while the relationships between linear sprinting performance and Dribble Deficit (r = −0.51–0.21) and between change-of-direction sprinting performance and Dribble Deficit (r = −0.14–0.26) were small and non-significant.ConclusionIn summary, Dribble Deficit reflects dribbling ability independent of sprinting ability and refines its application for practical use in assessing dribbling skills in female handball players.
Need-supportive coaching and engagement in Chinese high school football players: a serial mediation model
Authors: Jiang Chuantong , Razak Norsilawati Abdul , Rasyid Nelfianty Mohd 
Published: January 2025

IntroductionThis study aimed to investigate the relationship between need-supportive coaching behavior, subjective task value, goal orientation, and engagement among Chinese high school football players and propose four research hypotheses better to understand the determinants and mechanisms of athlete engagement.MethodsParticipants were 385 Chinese high school football players (228 boys; 157 girls; Mage = 16.07 years; SD = 1.15; range = 14–19 years) on 20 teams from 45 high schools using a proportional stratified random sampling method. Participants filled out previously validated questionnaires, including the Interpersonal Behaviors Questionnaire (IBQ), Subjective Task Value (STV), The Task and Ego Orientations Questionnaire (TEOSQ), and Athlete Engagement Questionnaire (AEQ). To address the hypothesis testing, this study utilized structural equation modeling (SEM) to analyze the proposed multiple mediation model.ResultsThe research results indicated that need-supportive coaching behavior had a significant direct (β = 0.121, Z = 3.667, p &lt; 0.001) and indirect (β = 0.209, Z = 5.500, p &lt; 0.001) effect on athlete engagement. Moreover, need-supportive coaching behavior positively predicted athlete engagement through subjective task value (β = 0.128, Z = 4.000, p &lt; 0.001) and task orientation (β = 0.053, Z = 3.118, p &lt; 0.001). Also, subjective task value and task orientation sequentially mediated the relationship between need-supportive coaching behavior and athlete engagement (β = 0.028, Z = 3.500, p &lt; 0.001).DiscussionThe findings demonstrate that need-supportive coaching behavior, subjective task value, and task orientation are important factors in promoting athlete engagement. The study implies that encouraging and guiding coaches to adopt a need-supportive coaching style is an effective motivational strategy, which can not only directly predict athlete engagement, but also indirectly influence athlete engagement through subjective task value and task orientation.