bluuebunny/crossref_metadata_2025_split huggingface dataset:
The origin of optical emission lines in the soft state of X-ray binary outbursts: the case of MAXI J1820+070
Authors: Koljonen K I I , Long K S , Matthews J H , Knigge C 
Published: March 2023

ABSTRACT
The optical emission line spectra of X-ray binaries (XRBs) are thought to be produced in an irradiated atmosphere, possibly the base of a wind, located above the outer accretion disc. However, the physical nature of – and physical conditions in – the line-forming region remain poorly understood. Here, we test the idea that the optical spectrum is formed in the transition region between the cool geometrically thin part of the disc near the mid-plane and a hot vertically extended atmosphere or outflow produced by X-ray irradiation. We first present a VLT X-Shooter spectrum of XRB MAXI J1820+070 in the soft state associated with its 2018 outburst, which displays a rich set of double-peaked hydrogen and helium recombination lines. Aided by ancillary X-ray spectra and reddening estimates, we then model this spectrum with the Monte Carlo radiative transfer code python, using a simple biconical disc wind model inspired by radiation-hydrodynamic simulations of irradiation-driven outflows from XRB discs. Such a model can qualitatively reproduce the observed features; nearly all of the optical emission arising from the transonic ‘transition region’ near the base of the wind. In this region, characteristic electron densities are on the order of 1012–13 cm−3, in line with the observed flat Balmer decrement (H $\alpha$/H $\beta$ ≈ 1.3). We conclude that strong irradiation can naturally give rise to both the optical line-forming layer in XRB discs and an overlying outflow/atmosphere that produces X-ray absorption lines.
Global N-body simulations of circumbinary planet formation around Kepler-16 and -34 analogues I: Exploring the pebble accretion scenario
Authors: Coleman Gavin A L , Nelson Richard P , Triaud Amaury H M J 
Published: March 2023

ABSTRACT
Numerous circumbinary planets have been discovered in surveys of transiting planets. Often, these planets are found to orbit near to the zone of dynamical instability, close to the central binary. The existence of these planets has been explained by hydrodynamical simulations that show that migrating circumbinary planets, embedded in circumbinary discs, halt at the central cavity that is formed by the central binary. Transit surveys are naturally most sensitive to finding circumbinary planets with the shortest orbital periods. The future promise of detecting longer period systems using radial-velocity searches, combined with the anticipated detection of numerous circumbinary planets by ESA’s PLATO mission, points to the need to model and understand the formation and evolution of circumbinary planets in a more general sense than has been considered before. With this goal in mind, we present a newly developed global model of circumbinary planet formation that is based on the mercury6 symplectic N-body integrator, combined with a model for the circumbinary disc and prescriptions for a range of processes involved in planet formation such as pebble accretion, gas envelope accretion, and migration. Our results show that under reasonable assumptions, the pebble accretion scenario can produce circumbinary systems that are similar to those observed, and in particular is able to produce planets akin to Kepler-16b and Kepler-34b. Comparing our results to other systems, we find that our models also adequately reproduce such systems, including multiplanet systems. Resonances between neighbouring planets are frequently obtained, whilst ejections of planets by the central binary acts as an effective source of free floating planets.
Biochemical characterisation of Mer3 helicase interactions and the protection of meiotic recombination intermediates
Authors: Altmannova Veronika , Firlej Magdalena , Müller Franziska , Janning Petra , Rauleder Rahel , Rousova Dorota , Schäffler Andreas , Bange Tanja , Weir John R 
Published: March 2023

Abstract
Crossing over between homologs is critical for the stable segregation of chromosomes during the first meiotic division. Saccharomyces cerevisiae Mer3 (HFM1 in mammals) is a SF2 helicase and member of the ZMM group of proteins, that facilitates the formation of the majority of crossovers during meiosis. Here, we describe the structural organisation of Mer3 and using AlphaFold modelling and XL-MS we further characterise the previously described interaction with Mlh1–Mlh2. We find that Mer3 also forms a previously undescribed complex with the recombination regulating factors Top3 and Rmi1 and that this interaction is competitive with Sgs1BLM helicase. Using in vitro reconstituted D-loop assays we show that Mer3 inhibits the anti-recombination activity of Sgs1 helicase, but only in the presence of Dmc1. Thus we provide a mechanism whereby Mer3 interacts with a network of proteins to protect Dmc1 derived D-loops from dissolution.
Rates of Age Verification for Cigarette and E-cigarette Purchases as a Function of State T21 Laws Before and After Implementation of the Federal T21 Law in the United States
Authors: Romm Katelyn F , Wang Yan , Schleicher Nina C , Pannel Alexandria , Williams River , Berg Carla J , Henriksen Lisa 
Published: March 2023

Abstract

Introduction
In the US, having a 21 minimum legal sales age for tobacco (T21) at the state level may have impacted age verification of cigarette and e-cigarette purchases among U.S. young adults (ages 18–26), before and/or after federal T21 implementation.

Aims and Methods
This study examined this by analyzing data from cigarette and/or e-cigarette users (n = 618 and n = 864) in six metropolitan areas in six states. Participants reported frequency of being age verified (“almost always” vs. less frequently) for cigarette and/or e-cigarette purchases across 3 timepoints (ie, wave 1 [w1]: September–December 2018, w2: September–December 2019, and w3: September–December 2020). Multilevel modeling examined time-varying state T21 status and time (reflecting federal T21 implementation) in relation to age verification of cigarette and e-cigarette purchases, respectively.

Results
The proportions almost always age verified for cigarette purchases in states with T21 versus without were: W1: 38.5% versus 37.7%, w2: 33.0% versus 39.1%, and w3: 45.4% versus 30.6%. For e-cigarettes, the proportions were: W1: 30.6% versus 40.3%, w2: 42.3% versus 50.5%, and w3: 56.0% versus 58.3%. In multilevel modeling, state T21 status was associated with greater likelihood of age verification for e-cigarettes (aOR = 1.67, CI = 1.13 to 2.45), but not for cigarettes. Age verification increased over time for e-cigarettes—both accounting for and not accounting for state T21 status. There were no changes for cigarettes.

Conclusions
State T21 status and time correlated with age verification for e-cigarettes, but not cigarettes. These self-reported age verification data contribute to evidence from compliance checks, indicating that retailers require additional prompts and enforcement to enhance compliance with T21 laws.

Implications
Current findings suggest that variations in regulations and gaps in enforcement may hinder the potential impact of increasing the minimum legal sales age, which ultimately may undermine the promise of such policies, specifically with regard to preventing tobacco use among the underage. Therefore, it is crucial to monitor retailer compliance with T21 laws and evaluate their efficacy to increase ID checks, minimize illegal sales, and curb underage use of tobacco. Relatedly, particular attention to enforcement efforts that may promote compliance is warranted.
Epidemiological Characteristics and Risk Factors of Severe Fever With Thrombocytopenia Syndrome in Yantai City, Shandong Province
Authors: Hou Shuting , Zhang Nannan , Liu Jingyu , Li Haiwen , Liu Xiuwei , Liu Tao 
Published: March 2023

AbstractBackgroundTo better understand the epidemiological characteristics and risk factors associated with the incidence of severe fever with thrombocytopenia syndrome (SFTS) in Yantai City, Shandong Province, China.MethodsThe SFTS data from 2010 to 2019 were obtained from the National Notifiable Disease Reporting System, and visualization was performed using ArcGIS 10. A community-based, 1:2 matched case-control study was conducted to investigate the risk factors for SFTS in Yantai City. Standardized questionnaires were used to collect detailed information about the demographics and risk factors for SFTSV infection.ResultsA total of 968 laboratory-confirmed SFTS cases were reported, 155 (16.01%) of which were fatal. The SFTS epidemic curve revealed that most cases occurred from May to August, accounting for 77.27% of all studied cases. The SFTS cases were mainly distributed in Lai Zhou, Penglai, Zhaoyuan, Haiyang, and Qixia from 2010 to 2019 (accounting for 83.47% of all cases). No differences in demographics were observed between the cases and controls. In the multivariate analysis, presence of rats in the household (odds ratio [OR] = 2.89, 95% confidence interval [CI] = 1.94–4.30), bitten by the ticks 1 month before the onset of symptoms (OR = 15.97, 95% CI = 5.36–47.60), and presence of weeds and shrubs around the house (OR = 1.70, 95% CI = 1.12–2.60) were found to be the risk factors for SFTS.ConclusionsOur results support the hypothesis that ticks are important vectors of the SFTS virus. Education on SFTS prevention and personal hygiene should be imparted in high-risk populations, especially among outdoor workers living in SFTS-endemic areas, while vector management should also be considered.
Energy metabolic pathways in neuronal development and function
Authors: Rumpf Sebastian , Sanal Neeraja , Marzano Marco 
Published: March 2023

Abstract
Neuronal development and function are known to be among the most energy-demanding functions of the body. Constant energetic support is therefore crucial at all stages of a neuron’s life. The two main adenosine triphosphate (ATP)-producing pathways in cells are glycolysis and oxidative phosphorylation. Glycolysis has a relatively low yield but provides fast ATP and enables the metabolic versatility needed in dividing neuronal stem cells. Oxidative phosphorylation, on the other hand, is highly efficient and therefore thought to provide most or all ATP in differentiated neurons. However, it has recently become clear that due to their distinct properties, both pathways are required to fully satisfy neuronal energy demands during development and function. Here, we provide an overview of how glycolysis and oxidative phosphorylation are used in neurons during development and function.
Devolution and the Prevent Strategy in Scotland: Constitutional Politics and the Path of Scottish P/CVE
Authors: Heath-Kelly Charlotte 
Published: March 2023

Abstract
This paper explores the implementation of the ‘Prevent Duty’ in Scotland. Using archival research into Parliamentary debates, as well as research interviews with Scottish government representatives and healthcare professionals, the paper sheds light on the constitutional politics surrounding the Counterterrorism and Security Act 2015 which resulted in the markedly different applications of Prevent between England and Scotland. The divergence of the policy between the nations, and the constitutional anomalies which facilitated a specifically Scottish Prevent program, have remained unaddressed in the academic literature—partly because of a mistaken assumption by researchers that the Prevent Strategy equally applies to all nations (given that Westminster legislates for the UK on matters of defence and security). To fill this gap in knowledge, this paper explores how the Scottish government was able to leverage the devolution settlement and associated constitutional conventions to implement a modest P/CVE program—dropping some components of the English and Welsh Prevent programs entirely. The paper contributes to studies on British constitutional conventions and the nature of inter-governmental politics in the UK by highlighting the surprising freedom to manoeuvre Scotland can enjoy, with regards to reserved policy areas.
Exploring Novel Polytubey Reproduction Pathways Utilizing Cumulative Genetic Tools
Authors: Sugi Naoya , Maruyama Daisuke 
Published: March 2023

Abstract
In the anthers and ovaries of flowers, pollen grains and embryo sacs are produced with uniform cell compositions. This stable gametogenesis enables elaborate interactions between male and female gametophytes after pollination, forming the highly successful sexual reproduction system in flowering plants. As most ovules are fertilized with a single pollen tube, the resulting genome set in the embryo and endosperm is determined in a single pattern by independent fertilization of the egg cell and central cell by two sperm cells. However, if ovules receive four sperm cells from two pollen tubes, the expected options for genome sets in the developing seeds would more than double. In wild-type Arabidopsis thaliana plants, around 5% of ovules receive two pollen tubes. Recent studies have elucidated the abnormal fertilization in supernumerary pollen tubes and sperm cells related to polytubey, polyspermy, heterofertilization and fertilization recovery. Analyses of model plants have begun to uncover the mechanisms underlying this new pollen tube biology. Here, we review unusual fertilization phenomena and propose several breeding applications for flowering plants. These arguments contribute to the remodeling of plant reproduction, a challenging concept that alters typical plant fertilization by utilizing the current genetic toolbox.
It's only natural: Plant respiration in unmanaged systems
Authors: Schmiege Stephanie C , Heskel Mary , Fan Yuzhen , Way Danielle A 
Published: March 2023

AbstractRespiration plays a key role in the terrestrial carbon cycle and is a fundamental metabolic process in all plant tissues and cells. We review respiration from the perspective of plants that grow in their natural habitat and how it is influenced by wide-ranging elements at different scales, from metabolic substrate availability to shifts in climate. Decades of field-based measurements have honed our understanding of the biological and environmental controls on leaf, root, stem, and whole-organism respiration. Despite this effort, there remain gaps in our knowledge within and across species and ecosystems, especially in more challenging-to-measure tissues like roots. Recent databases of respiration rates and associated leaf traits from species representing diverse biomes, plant functional types, and regional climates have allowed for a wider-lens view at modeling this important CO2 flux. We also re-analyze published data sets to show that maximum leaf respiration rates (R max) in species from around the globe are related both to leaf economic traits and environmental variables (precipitation and air temperature), but that root respiration does not follow the same latitudinal trends previously published for leaf data. We encourage the ecophysiological community to continue to expand their study of plant respiration in tissues that are difficult to measure and at the whole plant and ecosystem levels to address outstanding questions in the field.
Exploring the potential of Δ17O in CO2 for determining mesophyll conductance
Authors: Adnew Getachew Agmuas , Pons Thijs L , Koren Gerbrand , Peters Wouter , Röckmann Thomas 
Published: March 2023

AbstractMesophyll conductance to CO2 from the intercellular air space to the CO2–H2O exchange site has been estimated using δ18O measurements (gm18). However, the gm18 estimates are affected by the uncertainties in the δ18O of leaf water where the CO2–H2O exchange takes place and the degree of equilibration between CO2 and H2O. We show that measurements of Δ17O (i.e.Δ17O=δ17O−0.528×δ18O) can provide independent constraints on gm (gmΔ17) and that these gm estimates are less affected by fractionation processes during gas exchange. The gm calculations are applied to combined measurements of δ18O and Δ17O, and gas exchange in two C3 species, sunflower (Helianthus annuus L. cv. ‘sunny’) and ivy (Hedera hibernica L.), and the C4 species maize (Zea mays). The gm18 and gmΔ17 estimates agree within the combined errors (P-value, 0.876). Both approaches are associated with large errors when the isotopic composition in the intercellular air space becomes close to the CO2–H2O exchange site. Although variations in Δ17O are low, it can be measured with much higher precision compared with δ18O. Measuring gmΔ17 has a few advantages compared with gm18: (i) it is less sensitive to uncertainty in the isotopic composition of leaf water at the isotope exchange site and (ii) the relative change in the gm due to an assumed error in the equilibration fraction θeq is lower for gmΔ17 compared with gm18. Thus, using Δ17O can complement and improve the gm estimates in settings where the δ18O of leaf water varies strongly, affecting the δ18O (CO2) difference between the intercellular air space and the CO2–H2O exchange site.
SCARECROW maintains the stem cell niche in Arabidopsis roots by ensuring telomere integrity
Authors: Wang Bingxin , Shi Xiaowen , Gao Jingbo , Liao Rui , Fu Jing , Bai Juan , Cui Hongchang 
Published: March 2023

Abstract
Stem cells are the ultimate source of cells for various tissues and organs and thus are essential for postembryonic plant growth and development. SCARECROW (SCR) is a plant-specific transcription regulator well known for its role in stem cell renewal in plant roots, but the mechanism by which SCR exerts this function remains unclear. To address this question, we carried out a genetic screen for mutants that no longer express SCR in the stem cell niche of Arabidopsis (Arabidopsis thaliana) roots and characterized 1 of these mutants. Molecular genetics methods allowed us to pinpoint the causal mutation in this mutant in TELOMERIC PATHWAYS IN ASSOCIATION WITH STN 1 (TEN1), encoding a factor that protects telomere ends. Interestingly, TEN1 expression was dramatically reduced in the scr mutant. Telomerase and STN1 and CONSERVED TELOMERE MAINTENANCE COMPONENT 1 (CTC1), components of the same protein complex as TEN1, were also dramatically downregulated in scr. Loss of STN1, CTC1, and telomerase caused defects in root stem cells. These results together suggest that SCR maintains root stem cells by promoting expression of genes that ensure genome integrity. Supporting this conclusion, we demonstrated that the scr mutant accumulates more DNA damage than wild-type Arabidopsis and that this problem is aggravated after exposure to zeocin, a DNA damage reagent. Finally, we identified 2 previously uncharacterized motifs in TEN1 and provide evidence that a conserved amino acid residue in 1 of the motifs is indispensable for TEN1 function. SCR thus provides a connection between genome integrity and stem cell maintenance in Arabidopsis roots.
The microRNA ppe-miR393 mediates auxin-induced peach fruit softening by promoting ethylene production
Authors: Ma Li , Zhao Yingjie , Chen Miaojin , Li Yichen , Shen Zhijun , Cao Yang , Wu Di , Yu Mingliang , Grierson Donald , Shi Yanna , Chen Kunsong 
Published: March 2023

Abstract
Auxin can inhibit or promote fruit ripening, depending on the species. Melting flesh (MF) peach fruit (Prunus persica L. Batsch) cultivars produce high levels of ethylene caused by high concentrations of indole-3-acetic acid (IAA), which leads to rapid fruit softening at the late stage of development. In contrast, due to the low concentrations of IAA, the fruit of stony hard (SH) peach cultivars does not soften and produces little ethylene. Auxin seems necessary to trigger the biosynthesis of ethylene in peach fruit; however, the mechanism is not well understood. In this study, we identified miRNA gene family members ppe-miR393a and ppe-miR393b that are differentially expressed in SH and MF fruits. RNA ligase-mediated 5′ rapid amplification of cDNA ends and transient transformation of Nicotiana benthamiana revealed TRANSPORT INHIBITOR RESPONSE 1 (PpTIR1), part of the auxin perception and response system, as a target of ppe-miR393a and b. Yeast 2-hybrid assay and bimolecular fluorescence complementation assay revealed that PpTIR1 physically interacts with an Aux/IAA protein PpIAA13. The results of yeast 1-hybrid assay, electrophoretic mobility shift assay, and dual-luciferase assay indicated that PpIAA13 could directly bind to and trans-activate the promoter of 1-aminocyclopropane-1-carboxylic acid synthase 1 (PpACS1), required for ethylene biosynthesis. Transient overexpression and suppression of ppe-miR393a and PpIAA13 in peach fruit induced and repressed the expression of PpACS1, confirming their regulatory role in ethylene synthesis. Gene expression analysis in developing MF and SH fruits, combined with postharvest α-naphthalene acetic acid (NAA) treatment, supports a role for a ppe-miR393-PpTIR1-PpIAA13-PpACS1 module in regulating auxin-related differences in ethylene production and softening extent in different types of peach.
Sufficient coumarin accumulation improves apple resistance toCytospora maliunder high-potassium status
Authors: Du Youwei , Jia Hongchen , Yang Zi , Wang Shuanghong , Liu Yuanyuan , Ma Huiya , Liang Xiaofei , Wang Bo , Zhu Mingqi , Meng Yanan , Gleason Mark L , Hsiang Tom , Noorin Sadia , Zhang Rong , Sun Guangyu 
Published: March 2023

AbstractCytospora canker, caused by Cytospora mali, is the most destructive disease in production of apples (Malus domestica). Adding potassium (K) to apple trees can effectively control this disease. However, the underlying mechanisms of apple resistance to C. mali under high-K (HK) status remain unknown. Here, we found that HK (9.30 g/kg) apple tissues exhibited high disease resistance. The resistance was impeded when blocking K channels, leading to susceptibility even under HK conditions. We detected a suite of resistance events in HK apple tissues, including upregulation of resistance genes, callose deposition, and formation of ligno-suberized tissues. Further multiomics revealed that the phenylpropanoid pathway was reprogrammed by increasing K content from low-K (LK, 4.30 g/kg) status, leading to increases of 18 antifungal chemicals. Among them, the physiological concentration of coumarin (1,2-benzopyrone) became sufficient to inhibit C. mali growth in HK tissues, and exogenous application could improve the C. mali resistance of LK apple branches. Transgenic apple calli overexpressing beta-glucosidase 40 (MdBGLU40), which encodes the enzyme for coumarin synthesis, contained higher levels of coumarin and exhibited high resistance to C. mali even under LK conditions. Conversely, the suppression of MdBGLU40 through RNAi reduced coumarin content and resistance in HK apple calli, supporting the importance of coumarin accumulation in vivo for apple resistance. Moreover, we found that the upregulation of transcription factor MdMYB1r1 directly activated MdBGLU40 and the binding affinity of MdMYB1r1 to the MdBGLU40 promoter increased in HK apple tissue, leading to high levels of coumarin and resistance in HK apple. Overall, we found that the accumulation of defensive metabolites strengthened resistance in apple when raising K from insufficient to optimal status, and these results highlight the optimization of K content in fertilization practices as a disease management strategy.