bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preventing marine wildlife crime: An evaluation of legal protection and enforcement perspectives
Authors: Nurse Angus 
Published: April 2023

IntroductionDespite growing environmental awareness and the efforts of a variety of Non-Governmental Organisations (NGOs) to influence the wildlife protection policy agenda, wildlife laws remain outside the remit of mainstream criminal justice. Adopting a green criminological perspective, this article considers wildlife crime in respect of threats to UK marine wildlife. It examines the UK’s legal and enforcement framework and the scope and nature of both marine wildlife crime and harms to marine wildlife. Its core question is to consider the extent to which the UK’s wildlife law approach provides for effective wildlife protection and is adequate to deal with the threats facing marine wildlife.MethodsThe research is a mixed methods study that considers the relevant law, case law and reporting of wildlife crimes as well as the enforcement approach to marine wildlife crime. The article’s methodological approach is socio-legal in nature commencing with a review of the relevant literature on threats to marine wildlife and incorporating analysis of the current law and case law available via the legal databases BAILII and Westlaw. The study also conducted an analysis of licences for disturbance of marine wildlife via an examination of the open access case management database managed by the Marine Management Organisation (MMO) the main enforcement body for marine wildlife incidents in the UK.ResultsThe research identifies that while in principle the UK has robust legal protection for marine wildlife, in practice, policy allows exploitation and disturbance of marine wildlife that causes harm to individual animals and is detrimental to efforts to conserve marine wildlife and marine ecosystems.DiscussionThis research concludes that in the UK, potentially strong legislation existing on paper is arguably not backed by an effective enforcement regime. The remote nature of marine wildlife incidents, competition between marine wildlife and leisure and commercial activities and the very wording of legislation all create enforcement challenges. This article argues for marine wildlife crime to be integrated into mainstream crime policy linked to other forms of offending and criminal justice policy, rather than being largely seen as a purely environmental issue and a ‘fringe’ area of policing.
Clinical efficacy and safety of Cox-maze IV procedure for atrial fibrillation in patients with aortic valve calcification
Authors: Guo Ruikang , Fan Chengming , Sun Zhishan , Zhang Hao , Sun Yaqin , Song Long , Jiang Zenan , Liu Liming 
Published: April 2023

ObjectiveAtrial fibrillation is associated with a high incidence of heart valve disease. There are few prospective clinical research comparing aortic valve replacement with and without surgical ablation for safety and effectiveness. The purpose of this study was to compare the results of aortic valve replacement with and without the Cox-maze IV procedure in patients with calcific aortic valvular disease and atrial fibrillation.MethodsWe analyzed one hundred and eight patients with calcific aortic valve disease and atrial fibrillation who underwent aortic valve replacement. Patients were divided into concomitant Cox maze surgery (Cox-maze group) and no concomitant Cox-maze operation (no Cox-maze group). After surgery, freedom from atrial fibrillation recurrence and all-cause mortality were evaluated.ResultsFreedom from all-cause mortality after aortic valve replacement at 1 year was 100% in the Cox-maze group and 89%, respectively, in the no Cox-maze group. No Cox-maze group had a lower rate of freedom from atrial fibrillation recurrence and arrhythmia control than those in the Cox-maze group (P = 0.003 and P = 0.012, respectively). Pre-operatively higher systolic blood pressure (hazard ratio, 1.096; 95% CI, 1.004–1.196; P = 0.04) and post-operatively increased right atrium diameters (hazard ratio, 1.755; 95% CI, 1.182–2.604; P = 0.005) were associated with atrial fibrillation recurrence.ConclusionThe Cox-maze IV surgery combined with aortic valve replacement increased mid-term survival and decreased mid-term atrial fibrillation recurrence in patients with calcific aortic valve disease and atrial fibrillation. Pre-operatively higher systolic blood pressure and post-operatively increased right atrium diameters are associated with the prediction of recurrence of atrial fibrillation.
Exploring Swedish preschool teachers' perspectives on applying a self-reflection tool for improving inclusion in early childhood education and care
Authors: Ginner Hau Hanna , Selenius Heidi , Bjorck Eva 
Published: April 2023

IntroductionIn order to provide opportunities for high-quality early childhood education and care for each child, inclusive settings need to develop and sustain their potential to enable participation in terms of attendance and involvement for diverse groups of children. In 2015–2017, the European Agency for Special Needs and Inclusive Education completed a project on inclusive early childhood education, focusing on structures, processes, and outcomes that ensure a systemic approach to high-quality inclusive early childhood education. Within the project, a self-reflection tool for improving inclusion, the Inclusive Early Childhood Education Environment Self-Reflection Tool (ISRT), was developed. For purposes of future implementation of the ISRT, the present study focused on the teachers' perspective regarding the ISRT's potential to contribute to enabling all children's participation, defined as attending and being actively engaged in the activities in early childhood education and care. The specific aim was to explore Swedish preschool teachers' perceptions of the ISRT based on their experiences of applying the tool.MethodsTwelve preschool teachers participated in semi-structured interviews about their experiences of applying the tool. The interviews were analyzed with a thematic analysis.ResultsThe thematic analysis resulted in three main themes concerning the teachers' perception of (1) the construction of the ISRT, (2) the time required for using the tool, and (3) the tool's immediate relevance for practice. Each of these themes contained both negative and positive perceptions of the tool.DiscussionBased on the negative and positive perceptions identified in the three main themes, future research and development of the ISRT in Swedish preschools are discussed. On a general level, the results are discussed in relation to the implementation of the ISRT in terms of acceptability, appropriateness, and feasibility.
Perinatal exposure to the fungicide ketoconazole alters hypothalamic control of puberty in female rats
Authors: Franssen Delphine , Johansson Hanna K. L. , Lopez-Rodriguez David , Lavergne Arnaud , Terwagne Quentin , Boberg Julie , Christiansen Sofie , Svingen Terje , Parent Anne-Simone 
Published: April 2023

IntroductionEstrogenic endocrine disrupting chemicals (EDCs) such as diethylstilbestrol (DES) are known to alter the timing of puberty onset and reproductive function in females. Accumulating evidence suggests that steroid synthesis inhibitors such as ketoconazole (KTZ) or phthalates may also affect female reproductive health, however their mode of action is poorly understood. Because hypothalamic activity is very sensitive to sex steroids, we aimed at determining whether and how EDCs with different mode of action can alter the hypothalamic transcriptome and GnRH release in female rats.DesignFemale rats were exposed to KTZ or DES during perinatal (DES 3-6-12μg/kg.d; KTZ 3-6-12mg/kg.d), pubertal or adult periods (DES 3-12-48μg/kg.d; KTZ 3-12-48mg/kg.d).ResultsEx vivo study of GnRH pulsatility revealed that perinatal exposure to the highest doses of KTZ and DES delayed maturation of GnRH secretion before puberty, whereas pubertal or adult exposure had no effect on GnRH pulsatility. Hypothalamic transcriptome, studied by RNAsequencing in the preoptic area and in the mediobasal hypothalamus, was found to be very sensitive to perinatal exposure to all doses of KTZ before puberty with effects persisting until adulthood. Bioinformatic analysis with Ingenuity Pathway Analysis predicted “Creb signaling in Neurons” and “IGF-1 signaling” among the most downregulated pathways by all doses of KTZ and DES before puberty, and “PPARg” as a common upstream regulator driving gene expression changes. Deeper screening ofRNAseq datasets indicated that a high number of genes regulating the activity of the extrinsic GnRH pulse generator were consistently affected by all the doses of DES and KTZ before puberty. Several, including MKRN3, DNMT3 or Cbx7, showed similar alterations in expression at adulthood.ConclusionnRH secretion and the hypothalamic transcriptome are highly sensitive to perinatal exposure to both DES and KTZ. The identified pathways should be exploredfurther to identify biomarkers for future testing strategies for EDC identification and when enhancing the current standard information requirements in regulation.
The use of multiple datasets to identify autophagy-related molecular mechanisms in intracerebral hemorrhage
Authors: Xiao Yinggang , Zhang Yang , Wang Cunjin , Ge Yali , Gao Ju , Huang Tianfeng 
Published: April 2023

Background: Intracerebral hemorrhage (ICH) is a stroke syndrome with high mortality and disability rates, but autophagy’s mechanism in ICH is still unclear. We identified key autophagy genes in ICH by bioinformatics methods and explored their mechanisms.Methods: We downloaded ICH patient chip data from the Gene Expression Omnibus (GEO) database. Based on the GENE database, differentially expressed genes (DEGs) for autophagy were identified. We identified key genes through protein–protein interaction (PPI) network analysis and analyzed their associated pathways in Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). Gene-motif rankings, miRWalk and ENCORI databases were used to analyze the key gene transcription factor (TF) regulatory network and ceRNA network. Finally, relevant target pathways were obtained by gene set enrichment analysis (GSEA).Results: Eleven autophagy-related DEGs in ICH were obtained, and IL-1B, STAT3, NLRP3 and NOD2 were identified as key genes with clinical predictive value by PPI and receiver operating characteristic (ROC) curve analysis. The candidate gene expression level was significantly correlated with the immune infiltration level, and most of the key genes were positively correlated with the immune cell infiltration level. The key genes are mainly related to cytokine and receptor interactions, immune responses and other pathways. The ceRNA network predicted 8,654 interaction pairs (24 miRNAs and 2,952 lncRNAs).Conclusion: We used multiple bioinformatics datasets to identify IL-1B, STAT3, NLRP3 and NOD2 as key genes that contribute to the development of ICH.
Integrated analysis of multi-omics data reveals T cell exhaustion in sepsis
Authors: Li Qiaoke , Sun Mingze , Zhou Qi , Li Yulong , Xu Jinmei , Fan Hong 
Published: April 2023

BackgroundSepsis is a heterogeneous disease, therefore the single-gene-based biomarker is not sufficient to fully understand the disease. Higher-level biomarkers need to be explored to identify important pathways related to sepsis and evaluate their clinical significance.MethodsGene Set Enrichment Analysis (GSEA) was used to analyze the sepsis transcriptome to obtain the pathway-level expression. Limma was used to identify differentially expressed pathways. Tumor IMmune Estimation Resource (TIMER) was applied to estimate immune cell abundance. The Spearman correlation coefficient was used to find the relationships between pathways and immune cell abundance. Methylation and single-cell transcriptome data were also employed to identify important pathway genes. Log-rank test was performed to test the prognostic significance of pathways for patient survival probability. DSigDB was used to mine candidate drugs based on pathways. PyMol was used for 3-D structure visualization. LigPlot was used to plot the 2-D pose view for receptor-ligand interaction.ResultsEighty-four KEGG pathways were differentially expressed in sepsis patients compared to healthy controls. Of those, 10 pathways were associated with 28-day survival. Some pathways were significantly correlated with immune cell abundance and five pathways could be used to distinguish between systemic inflammatory response syndrome (SIRS), bacterial sepsis, and viral sepsis with Area Under the Curve (AUC) above 0.80. Seven related drugs were screened using survival-related pathways.ConclusionSepsis-related pathways can be utilized for disease subtyping, diagnosis, prognosis, and drug screening.
Machine learning for the prediction of all-cause mortality in patients with sepsis-associated acute kidney injury during hospitalization
Authors: Zhou Hongshan , Liu Leping , Zhao Qinyu , Jin Xin , Peng Zhangzhe , Wang Wei , Huang Ling , Xie Yanyun , Xu Hui , Tao Lijian , Xiao Xiangcheng , Nie Wannian , Liu Fang , Li Li , Yuan Qiongjing 
Published: April 2023

BackgroundSepsis-associated acute kidney injury (S-AKI) is considered to be associated with high morbidity and mortality, a commonly accepted model to predict mortality is urged consequently. This study used a machine learning model to identify vital variables associated with mortality in S-AKI patients in the hospital and predict the risk of death in the hospital. We hope that this model can help identify high-risk patients early and reasonably allocate medical resources in the intensive care unit (ICU).MethodsA total of 16,154 S-AKI patients from the Medical Information Mart for Intensive Care IV database were examined as the training set (80%) and the validation set (20%). Variables (129 in total) were collected, including basic patient information, diagnosis, clinical data, and medication records. We developed and validated machine learning models using 11 different algorithms and selected the one that performed the best. Afterward, recursive feature elimination was used to select key variables. Different indicators were used to compare the prediction performance of each model. The SHapley Additive exPlanations package was applied to interpret the best machine learning model in a web tool for clinicians to use. Finally, we collected clinical data of S-AKI patients from two hospitals for external validation.ResultsIn this study, 15 critical variables were finally selected, namely, urine output, maximum blood urea nitrogen, rate of injection of norepinephrine, maximum anion gap, maximum creatinine, maximum red blood cell volume distribution width, minimum international normalized ratio, maximum heart rate, maximum temperature, maximum respiratory rate, minimum fraction of inspired O2, minimum creatinine, minimum Glasgow Coma Scale, and diagnosis of diabetes and stroke. The categorical boosting algorithm model presented significantly better predictive performance [receiver operating characteristic (ROC): 0.83] than other models [accuracy (ACC): 75%, Youden index: 50%, sensitivity: 75%, specificity: 75%, F1 score: 0.56, positive predictive value (PPV): 44%, and negative predictive value (NPV): 92%]. External validation data from two hospitals in China were also well validated (ROC: 0.75).ConclusionsAfter selecting 15 crucial variables, a machine learning-based model for predicting the mortality of S-AKI patients was successfully established and the CatBoost model demonstrated best predictive performance.
Elevated circulating monocytes and monocyte activation in COVID-19 convalescent individuals
Authors: Park Juwon , Dean Logan S. , Jiyarom Boonyanudh , Gangcuangco Louie Mar , Shah Parthav , Awamura Thomas , Ching Lauren L. , Nerurkar Vivek R. , Chow Dominic C. , Igno Fritzie , Shikuma Cecilia M. , Devendra Gehan 
Published: April 2023

BackgroundMonocytes and macrophages play a pivotal role in inflammation during acute SARS-CoV-2 infection. However, their contribution to the development of post-acute sequelae of SARS-CoV-2 infection (PASC) are not fully elucidated.MethodsA cross-sectional study was conducted comparing plasma cytokine and monocyte levels among three groups: participants with pulmonary PASC (PPASC) with a reduced predicted diffusing capacity for carbon monoxide [DLCOc, &lt;80%; (PG)]; fully recovered from SARS-CoV-2 with no residual symptoms (recovered group, RG); and negative for SARS-CoV-2 (negative group, NG). The expressions of cytokines were measured in plasma of study cohort by Luminex assay. The percentages and numbers of monocyte subsets (classical, intermediate, and non-classical monocytes) and monocyte activation (defined by CD169 expression) were analyzed using flow cytometry analysis of peripheral blood mononuclear cells.ResultsPlasma IL-1Ra levels were elevated but FGF levels were reduced in PG compared to NG. Circulating monocytes and three subsets were significantly higher in PG and RG compared to NG. PG and RG exhibited higher levels of CD169+ monocyte counts and higher CD169 expression was detected in intermediate and non-classical monocytes from RG and PG than that found in NG. Further correlation analysis with CD169+ monocyte subsets revealed that CD169+ intermediate monocytes negatively correlated with DLCOc%, and CD169+ non-classical monocytes positively correlated with IL-1α, IL-1β, MIP-1α, Eotaxin, and IFN-γ.ConclusionThis study present evidence that COVID convalescents exhibit monocyte alteration beyond the acute COVID-19 infection period even in convalescents with no residual symptoms. Further, the results suggest that monocyte alteration and increased activated monocyte subsets may impact pulmonary function in COVID-19 convalescents. This observation will aid in understanding the immunopathologic feature of pulmonary PASC development, resolution, and subsequent therapeutic interventions.
Decreased breadth of the antibody response to the spike protein of SARS-CoV-2 after repeated vaccination
Authors: Horndler Lydia , Delgado Pilar , Romero-Pinedo Salvador , Quesada Marina , Balabanov Ivaylo , Laguna-Goya Rocío , Almendro-Vázquez Patricia , Llamas Miguel A. , Fresno Manuel , Paz-Artal Estela , van Santen Hisse M. , Álvarez-Fernández Stela , Olmo Asunción , Alarcón Balbino 
Published: April 2023

IntroductionThe rapid development of vaccines to prevent COVID-19 has raised the need to compare the capacity of different vaccines in terms of developing a protective humoral response. Previous studies have shown inconsistent results in this area, highlighting the importance of further research to evaluate the efficacy of different vaccines.MethodsThis study utilized a highly sensitive and reliable flow cytometry method to measure the titers of IgG1 isotype antibodies in the blood of healthy volunteers after receiving one or two doses of various vaccines administered in Spain. The method was also used to simultaneously measure the reactivity of antibodies to the S protein of the original Wuhan strain and variants B.1.1.7 (Alpha), B.1.617.2 (Delta), and B.1.617.1 (Kappa).ResultsSignificant differences were observed in the titer of anti-S antibodies produced after a first dose of the vaccines ChAdOx1 nCov-19/AstraZeneca, mRNA-1273/Moderna, BNT162b2/Pfizer-BioNTech, and Ad26.COV.S/Janssen. Furthermore, a relative reduction in the reactivity of the sera with the Alpha, Delta, and Kappa variants, compared to the Wuhan strain, was observed after the second boosting immunization.DiscussionThe findings of this study provide a comparison of different vaccines in terms of anti-S antibody generation and cast doubts on the convenience of repeated immunization with the same S protein sequence. The multiplexed capacity of the flow cytometry method utilized in this study allowed for a comprehensive evaluation of the efficacy of various vaccines in generating a protective humoral response. Future research could focus on the implications of these findings for the development of effective COVID-19 vaccination strategies.
Can stand the heat – ecology of the potentially invasive ascidian Styela plicata along the Mediterranean coast of Israel
Authors: Platin Raz , Shenkar Noa 
Published: April 2023

IntroductionBoth colonial and solitary ascidians (Chordata, Ascidiacea) have been gathering attention due to their impact on coastal environments. The solitary ascidian Styela plicata was first observed along the coast of Israel in 2014. It is considered a cosmopolitan species of unknown origin, and is well-known for its ability to create large aggregations on artificial substrates in the western Mediterranean Sea, North-Pacific Ocean and the Atlantic. The extreme environmental conditions that characterize the Mediterranean coast of Israel, with a salinity of 40 psu and sea-surface temperature reaching 33°C, may however present a barrier to its future spread.MethodsWe examined the seasonal reproduction patterns and population structure of three populations over two years, in addition to spatial surveys and monitoring oocyte release under laboratory conditions.ResultsHistological sections revealed significant differences in reproduction patterns between seasons and sites, with a higher proportion of the larger oocytes size group observed during the winter for all sites. Along the Israeli coast, with the exception of one site, S. plicata is currently restricted to marinas and ports.DiscussionWith an impressive ability to survive the extreme environmental conditions and sustain reproductive populations. Acquiring data on its spread and establishment is essential for modeling S. plicata’s distribution under the current and predicted global climate changes, and for developing tools for its mitigation and control.
Aphasic mild cognitive impairment in prodromal dementia with Lewy bodies
Authors: Watanabe Hiroyuki , Hikida Sakura , Ikeda Manabu , Mori Etsuro 
Published: April 2023

IntroductionThis study aimed to determine the characteristics of aphasic mild cognitive impairment (aphasic MCI), which is characterized by a progressive and relatively prominent language impairment compared with other cognitive impairments, in the prodromal phase of dementia with Lewy bodies (DLB).MethodsOf the 26 consecutive patients with aphasic MCI who had been prospectively recruited at our hospital, 8 patients were diagnosed with prodromal DLB and underwent language, neurological, neuropsychological, and neuroimaging (N-isopropyl-p-[123I] iodoamphetamine single-photon emission computed tomography; IMP-SPECT) testing. Three of these patients also underwent cholinesterase inhibitor therapy with donepezil.ResultsIn our aphasic MCI cohort, the clinical diagnosis of probable prodromal DLB accounted for more than 30% of cases; therefore, the presence of language impairment in prodromal DLB was not very uncommon. Five patients were diagnosed with progressive anomic aphasia and three with logopenic progressive aphasia. Anomic aphasia was characterized by apparent anomia but relatively preserved repetition and comprehension ability and logopenic progressive aphasia by anomia, phonemic paraphasia, and impaired repetition. IMP-SPECT revealed hypoperfusion of the temporal and parietal lobes in the left hemisphere in all but one patient. All patients who underwent cholinesterase inhibitor therapy with donepezil showed improvement in general cognitive function, including language function.DiscussionThe clinical and imaging features of aphasic MCI in prodromal DLB are similar to those observed in Alzheimer's disease. Progressive fluent aphasia, such as progressive anomic aphasia and logopenic progressive aphasia, is one of the clinical presentations in prodromal state of DLB. Our findings provide further insight into the clinical spectrum of prodromal DLB and may contribute to the development of medication for progressive aphasia caused by cholinergic insufficiency.