bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Graphene Oxide and Ground Granulated Blast Furnace Slag on Engineering Properties of High-Performance Concretes
Authors: Rezakhani D. , Jafari A. H. , Hajabasi M. 
Published: May 2023

Abstract
Concrete structures immersed in sea water suffer severe corrosion, and the application of nanomaterials to concrete presents an innovative approach to enhancing both its physical and mechanical properties. In this paper, the effects of combined admixtures of graphene oxide nanoplatelets (GONPs) and ground granulated blast furnace slag (GGBFS) on concrete were studied, and mechanical and physical tests were performed. It was known that the GGBFS admixture improves the durability of concrete, but in concrete samples containing both GONPs and GGBFS, the compressive strength increased by nearly 43 % and 46 % at 28 and 90 days of curing, respectively, compared with ordinary portland cement (OPC) concretes. The same admixing regime caused an increase in flexural strength of up to 58 % and 59 % during the same time spans. This was accompanied with a significant reduction in chloride permeability, which was appraised by a 64 % drop in electrical conductivity measurements. The GONPs additive, even at the very low concentrations, accelerated the formation of calcium silicate hydrate, thereby enhancing the mechanical and flexural strengths that directly affect the durability and water and chloride absorption. The overall durability and rebar corrosion resistance that were shown through electrochemical tests are consequences of the combined improvements in the physico-mechanical concrete properties. OPC concrete is thus both stronger and significantly more chloride ion resistant with additions of GONPs in the parts per million range and by replacing it with 50 percent by weight GGBFS. The enhanced pozzolanic reactions in concrete help to fill the porosity and significantly contribute to the curtailment mechanism.
Developmental Exposure to Polychlorinated Biphenyls Prevents Recovery from Noise-Induced Hearing Loss and Disrupts the Functional Organization of the Inferior Colliculus
Authors: Ibrahim Baher A. , Louie Jeremy J. , Shinagawa Yoshitaka , Xiao Gang , Asilador Alexander R. , Sable Helen J. K. , Schantz Susan L. , Llano Daniel A. 
Published: May 2023

Exposure to combinations of environmental toxins is growing in prevalence; and therefore, understanding their interactions is of increasing societal importance. Here, we examined the mechanisms by which two environmental toxins, polychlorinated biphenyls (PCBs) and high-amplitude acoustic noise, interact to produce dysfunction in central auditory processing. PCBs are well established to impose negative developmental impacts on hearing. However, it is not known whether developmental exposure to this ototoxin alters the sensitivity to other ototoxic exposures later in life. Here, male mice were exposed to PCBs in utero, and later as adults were exposed to 45 min of high-intensity noise. We then examined the impacts of the two exposures on hearing and the organization of the auditory midbrain using two-photon imaging and analysis of the expression of mediators of oxidative stress. We observed that developmental exposure to PCBs blocked hearing recovery from acoustic trauma.In vivotwo-photon imaging of the inferior colliculus (IC) revealed that this lack of recovery was associated with disruption of the tonotopic organization and reduction of inhibition in the auditory midbrain. In addition, expression analysis in the inferior colliculus revealed that reduced GABAergic inhibition was more prominent in animals with a lower capacity to mitigate oxidative stress. These data suggest that combined PCBs and noise exposure act nonlinearly to damage hearing and that this damage is associated with synaptic reorganization, and reduced capacity to limit oxidative stress. In addition, this work provides a new paradigm by which to understand nonlinear interactions between combinations of environmental toxins.SIGNIFICANCE STATEMENTExposure to common environmental toxins is a large and growing problem in the population. This work provides a new mechanistic understanding of how the prenatal and postnatal developmental changes induced by polychlorinated biphenyls (PCBs) could negatively impact the resilience of the brain to noise-induced hearing loss (NIHL) later in adulthood. The use of state-of-the-art tools, includingin vivomultiphoton microscopy of the midbrain helped in identifying the long-term central changes in the auditory system after the peripheral hearing damage induced by such environmental toxins. In addition, the novel combination of methods employed in this study will lead to additional advances in our understanding of mechanisms of central hearing loss in other contexts.
Ubx5‐Cdc48 assists the protease Wss1 at DNA‐protein crosslink sites in yeast
Authors: Noireterre Audrey , Serbyn Nataliia , Bagdiul Ivona , Stutz Françoise 
Published: May 2023

AbstractDNA‐protein crosslinks (DPCs) pose a serious threat to genome stability. The yeast proteases Wss1, 26S proteasome, and Ddi1 are safeguards of genome integrity by acting on a plethora of DNA‐bound proteins in different cellular contexts. The AAA ATPase Cdc48/p97 is known to assist Wss1/SPRTN in clearing DNA‐bound complexes; however, its contribution to DPC proteolysis remains unclear. Here, we show that the Cdc48 adaptor Ubx5 is detrimental in yeast mutants defective in DPC processing. Using an inducible site‐specific crosslink, we show that Ubx5 accumulates at persistent DPC lesions in the absence of Wss1, which prevents their efficient removal from the DNA. Abolishing Cdc48 binding or complete loss of Ubx5 suppresses sensitivity of wss1∆ cells to DPC‐inducing agents by favoring alternate repair pathways. We provide evidence for cooperation of Ubx5‐Cdc48 and Wss1 in the genotoxin‐induced degradation of RNA polymerase II (RNAPII), a described candidate substrate of Wss1. We propose that Ubx5‐Cdc48 assists Wss1 for proteolysis of a subset of DNA‐bound proteins. Together, our findings reveal a central role for Ubx5 in DPC clearance and repair.
Metastatic exocrine pancreatic adenocarcinoma in a giant otter (Pteronura brasiliensis)
Authors: Pinto Maria Helena Barros , Braga Thiago Rodrigues Cardoso , Blume Guilherme Reis , Oliveira Letícia Batelli de , Chagas Nicolas Thomas Costa das , Souza Fernanda Rezende , Cassali Geovanni Dantas , Sant’Ana Fabiano José Ferreira de 
Published: May 2023

AbstractNeoplasms of the exocrine pancreas are uncommon in domestic animals and rarely occur in wildlife. This article describes the clinical and pathological findings of one case of metastatic exocrine pancreatic adenocarcinoma in an 18-year-old giant otter (Pteronura brasiliensis) in captivity with a history of inappetence and apathy. Abdominal ultrasonography was inconclusive, and tomography revealed a neoplasm affecting the urinary bladder and hydroureter. During the anaesthesia recovery, the animal presented a cardiorespiratory arrest and died. Grossly, there were neoplastic nodules in the pancreas, urinary bladder, spleen, adrenal glands, and mediastinal lymph node. Microscopically, all nodules were composed of a malignant hypercellular proliferation of epithelial cells with acinar or solid disposition, supported by a sparse fibrovascular stroma. Neoplastic cells were immunolabeled with antibodies to Pan-CK, CK7, CK20, PPP and chromogranin A. Approximately 25% of the cells were positive for the presence of Ki-67 too. Pathological and immunohistochemical findings confirmed the diagnosis of metastatic exocrine pancreatic adenocarcinoma.
The effect of electrical stimulation of skeletal muscle on cardioprotection and on muscle-derived myokine levels in rats: A pilot study
Authors: Szabó Márton R. , Csont Tamás , Csonka Csaba 
Published: May 2023

AbstractElectrical muscle stimulation (EMS) is a widely used method in sports and rehabilitation therapies to simulate physical exercise. EMS treatment via skeletal muscle activity improves the cardiovascular functions and the overall physical condition of the patients. However, the cardioprotective effect of EMS has not been proven so far, therefore, the aim of this study was to investigate the potential cardiac conditioning effect of EMS in an animal model. Low-frequency 35-min EMS was applied to the gastrocnemius muscle of male Wistar rats for three consecutive days. Their isolated hearts were then subjected to 30 min global ischemia and 120 min reperfusion. At the end of reperfusion cardiac specific creatine kinase (CK-MB) and lactate dehydrogenase (LDH) enzyme release and myocardial infarct size were determined. Additionally, skeletal muscle-driven myokine expression and release were also assessed. Phosphorylation of cardioprotective signaling pathway members AKT, ERK1/2, and STAT3 proteins were also measured. EMS significantly attenuated cardiac LDH and CK-MB enzyme activities in the coronary effluents at the end of the ex vivo reperfusion. EMS treatment considerably altered the myokine content of the stimulated gastrocnemius muscle without altering circulating myokine levels in the serum. Additionally, phosphorylation of cardiac AKT, ERK1/2, and STAT3 was not significantly different in the two groups. Despite the lack of significant infarct size reduction, the EMS treatment seems to influence the course of cellular damage due to ischemia/reperfusion and favorably modifies skeletal muscle myokine expressions. Our results suggest that EMS may have a protective effect on the myocardium, however, further optimization is required.
Regression models for assessing the efficiency of vibratory separation of parsnip seeds taking into account air dynamics based on numerical simulation and field experiment
Authors: Nykyforov Аnton , Antoshchenkov Roman , Halych Ivan , Kis-Korkishchenko Liliia , Kis Viсtor , Dombrovska Alla , Kilimnik Inna 
Published: May 2023

To reduce the complexity of research into designing promising vibratory machines while minimizing the harmful effect of the aerodynamic factor, it is convenient to use regression models. With their help, a quantitative assessment of the effectiveness of separation (cleaning) of seed mixtures is carried out, depending on the design parameters and the mode of operation of vibratory machines.
This paper reports the results of research on the construction of regression models for parsnip seeds based on numerical modeling and full-scale experiment. Based on numerical modeling, a four-factor regression model of the second order was built, which takes into account the geometric characteristics of the aerodynamic screen, the design of the set of working surfaces, and the oscillation amplitude of a vibratory machine. Based on a full-scale experiment, a three-factor regression model of the second order was constructed for a constant gap between the working surfaces.
A comparative analysis of the resulting regression models suggests that numerical modeling provides satisfactory accuracy in assessing the influence of the aerodynamic factor. This estimate, when using a regression model based on a numerical experiment, exaggerates the estimate determined by the full-scale experiment by 5–15 % (depending on the regressate variation area localization).
Hence, the numerical model of the process of vibrational motion of light-weight seeds, taking into account the action of aerodynamic forces and moments, used to build a regression model of separation of parsnip seeds, can be considered adequate. Regression models (for parsnips and other plant crops), which are built on the basis of numerical modeling, should be used to solve problems of optimizing the parameters of vibratory machines according to the criterion of reducing the harmful effect of the aerodynamic factor.
Determining the possibility of the appearance of a combustible medium in the hydrogen storage and supply system
Authors: Abramov Yuriy , Basmanov Oleksii , Krivtsova Valentina , Mikhayluk Andriy , Khmyrov Ihor 
Published: May 2023

The object of this study is the process of functioning of the hydrogen storage and supply system. The issue of fire-explosive events in the hydrogen storage and supply system is investigated. A set of mathematical models has been built to determine the probability of a combustible medium in the hydrogen storage and supply system. This set includes partial mathematical models for the main elements of the system, which are united by a generalized mathematical model. When constructing partial mathematical models, the probabilities of trouble-free operation of the main elements of the system are used, which include a pipeline and a gas generator with a pressure stabilization circuit. The probability of trouble-free operation is represented in the form of two multiplicative components that take into account catastrophic and parametric failures of the main elements of the system. When determining the probability of trouble-free operation of the main elements of the system in relation to parametric failures, the integral (generalized) parameters were used. In particular, for a gas generator, such parameters are its time constants. The current values of time constants of the gas generator are determined according to the developed algorithm whose feature is the use for its implementation of the values of the amplitude-frequency characteristics of the system, which are determined at three a priori given frequencies. For a typical version of the on-board hydrogen storage and supply system, quantitative indicators of the likelihood of a combustible medium are given. It is shown that if the parametric failures of the main elements of the system are not taken into account, an error occurs, the value of which is 30.0 %.
The results could be used to obtain an express assessment of the level of fire hazard of hydrogen storage and supply systems at different stages of their life cycle.
Development of the method of constructing the expander turbine rotation speed regulator
Authors: Kulinchenko Heorhii , Zhurba Viacheslav , Panych Andrii , Leontiev Petro 
Published: May 2023

The study is devoted to the expander turbine rotation speed regulator, considering the possibility of implementing this regulator on microprocessor automation tools. The use of expander-generator units in general improves energy saving indicators, and the ability to maintain the turbine shaft rotation speed within the specified limits, in turn, directly affects the indicators of the quality of the generated electricity. The expander turbine, as a control object, is described by non-linear equations, which determines the possibility of using regulators of different designs, and requires the selection of the most suitable one according to certain criteria. As part of the study, based on the tasks of practical implementation of the regulator on microprocessor devices, the expediency of reducing the transfer function of the model in the process of identifying the control object was confirmed. As a result of research on an experimental setup, it is shown that the use of a three-position relay regulator allows for regulation dynamics at the level of a classic PID regulator. An important result of the research is the stabilization of the turbine rotation speed, which affects the parameters of the electricity generated by the generator. The description of the control object was linearized by constructing a family of transfer functions for the operating points of the control range. For the construction of the turbine rotation speed regulator, the criterion of "minimum fluctuation of the parameter when changing its set value" is proposed. A regulator for a non-linear object with oscillatory features is built, which has a simple implementation and a cycle time of 1 ms. It makes it possible to reduce rotation speed fluctuations to 5 % and minimize the impact of rotation process disturbances
Afterslip on Conjugate Faults of the 2020 Mw 6.3 Nima Earthquake in the Central Tibetan Plateau: Evidence from InSAR Measurements
Authors: Hong Shunying , Liu Mian , Zhou Xin , Meng Guojie , Dong Yanfang 
Published: May 2023

ABSTRACT
Afterslip could help to reveal seismogenic fault structure. The 2020 Mw 6.3 Nima earthquake happened in a pull-apart basin within the Qiangtang block, central Tibetan plateau. Previous studies have explained the coseismic and early (&lt;6 mo) postseismic deformation by rupture and afterslip on a normal fault bounding the western side of the basin. Here, we resolved the 19-month Interferometric Synthetic Aperture Radar-measured sequences of postseismic displacements that revealed a second postseismic displacement center ~12 km to the east of the main fault. Fitting the postseismic displacement requires afterslip on both the main fault and an antithetic fault that probably forms a y-shaped pair of conjugate faults in a negative flower structure. Stress-driven afterslip models suggest that the required afterslip on the antithetic fault could be triggered by coseismic rupture of the main fault or by a simultaneous rupture on the antithetic fault. The afterslip on both faults occurred mainly up-dip to the coseismic slip and has released moment ~15%–19% of that by the coseismic rupture. These results provide insights into active extension in the central Tibetan plateau and highlight the complex nature of fault rupture and afterslip.
Kajian Fenotip Kambing Senduro sebagai Kekayaan Sumber Daya Genetik Ternak Lokal Indonesia
Authors: Amam Amam , Rifa'i Rifa'i , Surjowardojo Puguh , Susilorini Tri Eko 
Published: May 2023

ABSTRACT. Galur kambing Senduro ditetapkan melalui Keputusan Menteri Pertanian Republik Indonesia Nomor 1055/Kpts/SR.120/10/2014. Kambing Senduro sebagai kekayaan sumber daya genetik ternak lokal Indonesia memiliki fenotip yang dapat diukur berdasarkan sifat kuantitatifnya. Penelitian pertama bertujuan untuk mengkaji fenotip kambing Senduro betina berdasarkan pada tinggi pundak, panjang badan, lingkar dada, bobot badan, dan panjang telinga. Penelitian kedua bertujuan menghubungkan sifat kuantitatif dengan umur ternak. Penelitian pertama menggunakan metode observasi, sedangkan penelitian kedua menggunakan metode korelasi. Data penelitian ditabulasi dan dianalisis secara deskriptif kuantitatif dan analisis korelasi dengan menggunakan Microsoft Excel 2010 dan SPSS 26.0. Pengamatan dilakukan pada 102 ekor kambing Senduro betina. Hasil penelitian menunjukkan bahwa kambing Senduro betina memiliki tinggi pundak 70-80 cm (55,88%), panjang badan 70-80 cm (53,92%), lingkar dada 80-90 cm (59,80%), bobot badan 50-60 kg (32,35%), dan panjang telinga 30-40 cm (69,61%). Panjang badan, lingkar dada, dan bobot badan memiliki korelasi positif yang sangat kuat dengan umur ternak. Penelitian ini merekomendasikan bahwa perlu adanya upaya pembaharuan data dan informasi terkait fenotip kambing Senduro yang berkaitan dengan sifat kuantitatif ternak yang mengacu pada Keputusan Menteri Pertanian Republik Indonesia Nomor 1055/Kpts/SR.120/10/2014. (Phenotype study of Senduro goats as wealth of Indonesian local livestock genetic resources) ABSTRAK. The Senduro goat breed was determined through the Decree of the Minister of Agriculture of the Republic of Indonesia Number 1055/Kpts/SR.120/10/2014. The Senduro goat as a wealth of genetic resources for local Indonesian livestock has a phenotype that can be measured based on its quantitative characteristics. The first study aimed to examine the phenotype of female Senduro goats based on shoulder height, body length, chest circumference, body weight, and ear length. The second study aimed to evaluate quantitative characteristics with the age of Senduro goat. The first research uses the observation method, while the second research uses the correlation method. Observations were made on 102 female Senduro goats. The results showed that female Senduro goats had a shoulder height of 70-80 cm (55.88%), body length 70-80 cm (53.92%), chest circumference 80-90 cm (59.80%), body weight 50 -60 kg (32.35%), and ear length 30-40 cm (69.61%). Body length, chest circumference, and body weight have a very strong positive correlation with the age of Senduro goat. This study recommends that efforts to update data and information related to the phenotype of the Senduro goat related to the quantitative characteristics of Senduro goat are needed which refers to the Decree of the Minister of Agriculture of the Republic of Indonesia Number 1055/Kpts/SR.120/10/2014.
Perbedaan Keberhasilan Inseminasi Buatan Menggunakan Metode Dosis Tunggal dan Ganda pada Sapi Madura
Authors: Rasyad Khairul , Yekti Aulia Puspita Anugra , Kuswati Kuswati , Susilawati Trinil 
Published: May 2023

ABSTRACT. Penelitian ini bertujuan mengevaluasi keberhasilan IB menggunakan metode dosis tunggal dan ganda pada sapi Madura. Penelitian menggunakan 25 ekor dengan metode IB dosis tunggal dan 25 ekor dengan metode IB dosis ganda. IB menggunakan semen beku sapi Madura yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Teknik deposisi semen adalah 4+ (deep insemination) yaitu pada posisi cornua uteri selanjutnya dilakukan IB dosis tunggal (jam ke-8 setelah menunjukkan tanda-tanda berahi) dan IB dosis ganda (jam ke-2 dan ke-8 setelah menunjukkan tanda-tanda berahi). Akseptor diinjeksi dengan Bio ATP+ merek “Rheinbio” 10 ml per ekor dan pemberian pakan konsentrat merek “Novo NC62” 1 kg per ekor per hari selama tujuh hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Conception Rate (CR), dan Service Per Conception (S/C). Hasil penelitian menunjukkan bahwa IB dosis tunggal menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 80%, CR sebesar 60%, dan S/C sebesar 1,71. Sedangkan pada IB dosis ganda menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 84%, CR sebesar 68%, dan S/C sebesar 1,53. Kesimpulan penelitian adalah nilai NRR, CR, dan S/C pada sapi Madura dengan IB dosis ganda lebih baik daripada dengan IB dosis tunggal, dan IB dosis ganda dapat meningkatkan conception rate pada sapi Madura. (The success different of artificial insemination using single and double dosage method of Madura cows) ABSTRAK. This study aims to evaluate the success of AI using methods single and double dosage plus in Madura cows. The study used 25 Madura cow using the AI method single dosage and 25 Madura cow using the AI method double dosage. AI uses frozen semen from Madura cows produced by the Singosari Center for Artificial Insemination. The semen deposition technique is 4+ (deep insemination) in the cornua uteri position then AI implemented with a single dosage (8th hours after showing signs of estrus) and double dosage (2nd and 8th hours after showing signs of estrus). The acceptors were injected with 10 ml of "Rheinbio'' brand Bio ATP+ per cow and 1 kg of concentrate "Novo NC62" brand per cow per day was given for seven days after IB. Research variables include Non Return Rate (NRR), Conception Rate (CR), and Service Per Conception (S/C). The results showed that AI of single dosage had value NRR 1 of 92%, NRR 2 of 80%, CR of 60%, and S/C of 1.71. While AI of double dosage had value NRR 1 of 92%, NRR 2 of 84%, CR of 68%, and S/C of 1.53. In conclusion, the value of NRR, CR, and S/C in Madura cows with AI double dosage was better than AI single dosage, and AI double dosage could increase the conception rate in Madura cows.