bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Surgical Treatment for Medication- Related Osteonecrosis of the Jaw
Authors: Tu Chengwei , Li Na , Li Dashuai , Zheng Shunyou , Ding Xi , Wu Gang , Liu Xueying 
Published: May 2023

Abstract
Objective To investigate the efficacy of surgical treatment for medication-related osteonecrosis of the jaw (MRONJ).
Methods Twenty-eight patients with MRONJ who underwent surgical operation in the Department of Oral and Maxillofacial Surgery of the First Affiliated Hospital of Wenzhou Medical University from November 2016 to August 2021 were enrolled, and a retrospective analysis of clinical data (gender, age at onset, cause of onset, disease stage, treatment and prognosis, etc.）was performed. In 20 patients with stage 2 lesions, partial jaw resection was performed, and the soft tissues were tightly sutured under tension-free conditions, including direct sutures and indirect sutures using adjacent flaps with or without absorbable collagen membrane. In 8 cases of stage 3 lesions, sequestrum was performed and a drainage channel was formed. Regular follow-up was performed after operation, and the surgical efficacy was evaluated according to the clinical manifestations, pain scores, and X-ray examinations of the patients.
Results There were 13 male and 15 female MRONJ patients respectively, and the age at onset was (69.18±7.42) years old. According to the guidelines of the American Association of Oral and Maxillofacial Surgery (AAOMS), the patients were divided into stage 2 and 3. As for the stage 2 MRONJ patients, who were followed up for 10-53 months after operation, the rate of unplanned reoperation was 10% (1/20), and the pain score at the 3rd month after operation was [(0.35±0.49) points], which was significantly lower than the preoperative pain score [(3.40±0.82) points] (P<0.05). The unplanned reoperation rate of MRONJ patients in stage 3 was 0% during the follow-up of 19-84 months, and the pain score at the 3rd month after operation was [(0.63±0.52) points], significantly lower than the preoperative one [(5.37±1.06) points] (P<0.05).
Conclusions Surgical treatment is necessary to cure MRONJ for patients who has failed non-surgical treatment. For patients with stage 2 MRONJ, complete removal of necrotic bone and tension-free closure of mucosal wounds are the keys to successful surgery. For stage 3 MRONJ patients, the goal is to remove sequestrum, establish drainage, relieve pain, and prevent worse progression of the disease.
Silver nanoparticles induce cytotoxicity by releasing Ag + from the lysosome and increasing lysosomal membrane permeability
Authors: Peng Yinbo , Ni Wei , Ni Tao , Xu Peng , Gu Chuan , Yu Weirong , Xie Aiguo , Yao Min 
Published: May 2023

Abstract
Silver nanoparticles (AgNPs) have been widely used in many productions. Previous studies have shown partly AgNPs cytotoxicity in vitro and in vivo; however, the mechanism of this cytotoxicity has not been identified. Our study proved that AgNPs reached the lysosomes after contact with human fibroblasts. Cytotoxicity gradually increased as AgNPs enrichment in the lysosomes, accompanied by a reduction in lysosomal membrane permeability (LMP) and an increase in intracellular silver ion (Ag+). Inhibiting LMP or chelating Ag+ can effectively reduce AgNP toxicity. It has been confirmed that AgNPs gradually increased in the liver and spleen after subcutaneous injection, accompanied by the abnormal of liver function. Inhibition of LMP or chelation of Ag+in vivo can effectively protect liver and renal functions, and this protective effects showed a good synergistic effect. Our studies will provide theoretical support for more reasonable and safe clinical applications of AgNPs.
Treatment of Dairy Effluent by Novel Bacteria
Authors: Ponraj Aarthi , Sriniva Hemalatha , Ibrahim Faridha Begum 
Published: May 2023

Abstract
The main cause of river contamination is the dairy sector. Sludge and effluents from food-related businesses, particularly those in the dairy sector, contain a sizable amount of organic matter, fatty acids, and nitrogenous chemicals. Whey is the primary pollutant in milk processing effluent due to its enormous volume and high organic content. Between 85% and 95% of the milk's weight and 55% of its constituent parts are made up of it. Lactose makes up the majority of the 4-5% of carbs in whey. In this work, the biological approach was used to identify Enterobacter cloacae and Aeromonas intestinalis as Dairy-degrading bacteria. The analysis of optimizing several parameters for the degradation of dairy effluent. Instrument Analysis also confirmed the Structural Changes of Degradation in Dairy effluent.
Polymorphisms of XRCC2 gene and breast cancer risk : a case-control study in Han women of northwest China
Authors: Wang Haitao , Wang Tao , Wang Lan , Guo Hongyun , Yang Suisheng , Zhu Gongjian , Wei Changsheng , Mao Aihong , Min Jianping , Guo Huan , Zhou Haihong , Hu Qingrong , Bai Xiaorong , Su Haixiang 
Published: May 2023

Abstract
Background The XRCC2 gene plays an important role in the homologous recombination repair of DNA double strand breaks. Functional single nucleotide polymorphisms (SNPs) of XRCC2 may alter DNA repair capacity and affect cancer susceptibility. This study aims to evaluate the association between five tagSNPs in XRCC2 and breast cancer susceptibility in Han women of northwest China.
Methods A total of 514 histopathologically confirmed breast cancer patients and 1028 region- and age-matched cancer-free controls provided blood samples for analysis of SNP genotypes in this case-control study using a QuantStudio™ 12K Flex Real-Time PCR System.
Results After adjusting covariates, 2 SNPs in XRCC2 (rs3218536 and rs6964582) showed significant associations with the risk of breast cancer. In haplotype analysis, CGTTC haplotype conferred an increased risk of breast cancer(P=5.83×10-3), whereas TGTTA haplotype was associated with a decreased risk of breast cancer(P=1.17×10-3). Significant associations were shown between CGTTA, CCTTA haplotypes of XRCC2 and Her-2 statuses(P=2.15×10-3; P=4.06×10-3, respectively). Interaction analysis showed that the three-loci model (rs3218438, rs3218536 and rs6964582) was the best with the maximal testing balance accuracy of 0.6597, and the maximal 100% cross-validation consistency (P=0.001).
Conclusion These results indicate that XRCC2 gene polymorphisms may affect the risk of breast cancer and show that some SNPs and haplotypes in XRCC2 are associated with breast cancer characteristics in the Han population of northwest China.
Impacts of the invasive Impatiens glandulifera on the growth of three native plants
Authors: Mynard Poppy , Vilas Julia Sanchez 
Published: May 2023

Abstract
Himalayan balsam (Impatiens glandulifera) is a highly invasive annual herb that has spread rapidly throughout the UK. This species has allelopathic potential – chemicals released into the soil that can reduce seed germination and growth of neighbouring plants. Allelopathy and resource competition are key contributors to the success of this species; however, little is still known about the effects of litter of I. glandulifera on native species. This is important because in the invaded range, I. glandulifera frequently grows in monotypic stands that die back in autumn leaving large amounts of litter. Here, we aim to investigate the effects that seedlings and residues (above-ground plant matter) of I. glandulifera have on the chlorophyll content and growth of co-occurring native species, namely, Trifolium pratense, Linum grandiflora, and Silene dioica. We found reduced chlorophyll content and growth (measured as above-ground dry mass) in the three native species studied in response to growing with a I. glandulifera individual. However, the presence of residues of this invasive in the soil did not reduce the chlorophyll content and growth of the native species but rather had positive effects for S. dioica – which increased the above-ground dry mass in response to the addition of plant matter in the soil. Our results confirm the competitive superiority of I. glandulifera over native plants and highlight potential benefits of the litter of this invasive on the growth of natives.
Dysregulation of ferroptosis-related genes in granulosa cells associates with impaired oocyte quality in polycystic ovary syndrome
Authors: Huang Jialyu , Fan Hancheng , Li Chenxi , Yang Kangping , Xiong Chaoyi , Xiong Siyi , Feng Shenghui , Chen Shen , Wang Bangqi , Su Yufang , Xu Boyun , Yang Haiyan , Wang Ni , Zhu Jing 
Published: May 2023

Abstract
Background Poor oocyte quality remains one of the major challenges for polycystic ovary syndrome (PCOS) patients during in vitro fertilization (IVF) treatment. Granulosa cells (GCs) in PCOS display altered functions and could cause an unfavorable microenvironment for oocyte growth and maturation. Ferroptosis is a new form of programmed cell death, but its role in PCOS has been largely unclarified.Methods Ferroptosis-related differentially expressed genes (DEGs) in PCOS GCs were identified by bioinformatic analyses of GSE155489 and GSE168404 datasets. Functional enrichment analyses were conducted using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Core ferroptosis-related genes were further screened by random forest, and evaluated for diagnostic value by receiver operating characteristic curve analyses. Gene expression was validated by real-time quantitative polymerase chain reaction of collected GC samples, and analyzed for association with oocyte quality. In addition, gene regulatory network was constructed based on predicted RNA interactions and transcription factors, while potential therapeutic compounds were screened through molecular docking with crystallographic protein structures.Results A total of 14 ferroptosis-related DEGs were identified. These DEGs were mainly enriched in reactive oxygen species metabolic process, mitochondrial outer membrane, antioxidant activity as well as ferroptosis and adipocytokine signaling pathways. Eight core ferroptosis-related genes (ATF3, BNIP3, DDIT4, LPIN1, NOS2, NQO1, SLC2A1 and SLC2A6) were further selected in random forest model, which showed high diagnostic performance for PCOS. Seven of them were validated in GC samples, and five were found to significantly and positively correlated with one or more oocyte quality parameters in PCOS patients, including oocyte retrieval rate, mature oocyte rate, normal fertilization rate, and good-quality embryo rate. Gene regulatory network revealed JUN and HMGA1 as two important transcription factors, while dicoumarol and flavin adenine dinucleotide were predicted as small molecules with therapeutic potential.Conclusions This is the first comprehensive report to study the differential expression of ferroptosis-related genes in GCs of PCOS and their clinical relevance with oocyte quality. Our findings could provide novel insights on the potential role of GC ferroptosis in PCOS pathogenesis, diagnosis, and targeted treatment.
New modified heterocyclic-magnetite chitosan nanocomposite for efficient alizarin red dye removal: adsorption analysis and antibacterial activity.
Authors: Abuessawy Asmaa Abuessawy , Fouda Amr Fouda , Abdel-Rahman Adel A.-H. Abdel-Rahman , Hawata Mohamed A. Hawata , Hamad Nora A. Hamad 
Published: May 2023

Abstract
Alizarin Red (AR) is one of the most colored hazardous industrial dye. For effective removal of AR, a new sorbent of modified heterocyclic- magnetite chitosan nanocomposite (AOC@MC) was synthesized and characterized by FTIR, TGA, and SEM-EDX Effect of contact time, pH, adsorbent dosage, initial Alizarine Red concentration and temperature was investigated. The maximum AR removal was 98.9% and attained at optimum conditions which were pH = 3.0, contact time = 50 min., and adsorbent dosage = 0.03 g/L. The €findings revealed that at pH 3 and 30◦ C, the maximum adsorption capacity was approximately 162 mg/g. Within 50 min, equilibrium adsorption was attained. A pseudo-second-order equation might be used to fit the kinetic data that was acquired at the optimal pH level of 3. Langmuir adsorption isotherms could accurately represent the adsorption process. The antimicrobial properties of the functionalized sorbent and its heterocyclic base are characterized by determining the zone of inhibition (ZOI) against Staphylococcus aureus and Klebsiella pneumonia as a model for Gram-positive and Gram-negative bacteria respectively. The modified chitosan sorbent (AOC@MC) exhibits promising activity with clear zones of 20.1 ± 0.2 and 17.6 ± 0.4 mm for S. aureus and K. pneumonia respectively. Whereas, the synthesized heterocyclic compounds didn’t show antibacterial activity toward tested strains. Overall, the modified compound was efficient on AR dye removal and antibacterial activity compared to unmodified and heterocyclic compounds.
Multipath-guided heterogeneous graph neural networks for sequential recommendation
Authors: Yin Fulian , Ji Meiqi , Xing Tongtong , Yao Zebin , Fu Ruiling , Wu Yuewei 
Published: May 2023

Abstract
With the explosion of information and users’ changing interest, program sequential recommendation becomes increasingly important for TV program platforms to help their users find interesting programs. Existing sequential recommendation methods mainly focus on modeling user preferences from users’ historical interaction behaviors directly, with insufficient learning about the dynamics of programs and users, while ignoring the rich semantic information from the heterogeneous graph. To address these issues, we propose the multipath-guided heterogeneous graph neural networks for TV program sequential recommendation (MHG-PSR), which can enhance the representations of programs and users through multiple paths in heterogeneous graphs. In our method, the auxiliary information is fused to supplement the semantics of program and user to obtain initial representations. Then, we explore the interactive behaviors of programs and users with temporal and auxiliary information to model the collaborative signals in the heterogeneous graph and extract the users’ dynamic preferences of programs. Extensive experiments on real-world datasets verify the proposed method can effectively improve the performance of TV program sequential recommendation.
Augmentation of learning in schizophrenia by D-serine is related to auditory and frontally-generated biomarkers: A randomized, double-blind, placebo-controlled study
Authors: Govani Viraj , Shastry Adithya , Iosifescu Daniel , Govil Preetika , Mayer Megan , Sobeih Tarek , Choo Tse , Wall Melanie , Sehatpour Pejman , Kantrowitz Joshua 
Published: May 2023

Abstract
Auditory cognition is impaired in schizophrenia, and typically engages a complex, distributed, hierarchical network, including both auditory and frontal input. We recently demonstrated proof of principle for the target engagement of an N-methyl-D-aspartate-type glutamate receptor (NMDAR) agonist + auditory targeted remediation (d-serine+AudRem) combination, showing significant improvement in auditory-learning induced plasticity and mismatch negativity. In this secondary analysis, we report on frontal EEG outcomes, assessing for both generalized effects and the mechanism of auditory plasticity.
21 schizophrenia or schizoaffective disorder participants were randomized to three 1x weekly AudRem + double-blind d-serine (100 mg/kg) visits. In AudRem, participants indicated which paired tone was higher in pitch. The focus of this secondary analysis was a frontally (premotor) mediated EEG outcome— event-related desynchronization in the b band (b-ERD), which was shown to be sensitive to AudRem in previous studies.
d-Serine+AudRem led to significant improvement in b-ERD power across the retention and motor preparation intervals (F1,18=6.0, p=0.025) vs. AudRem alone. b-ERD was significantly related to baseline cognition, but not auditory-learning induced plasticity.
The principal finding of this prespecified secondary analysis are that in addition to improving auditory based biomarkers, the d-serine+AudRem combination led to significant improvement in biomarkers thought to represent frontally mediated dysfunction, suggesting potential generalization of effects. Changes in auditory-learning induced plasticity were independent of these frontally mediated biomarkers. Ongoing work will assess whether d-serine+AudRem is sufficient to remediate cognition or whether targeting frontal NMDAR deficits with higher-level remediation may also be required.
Trial Registration: NCT03711500
SLC25A24, a potential and novel biomarker for diagnosing colorectal cancer
Authors: Xu Haitao , Liu Yanyan , Liu Shan , An Qian , Cao Huijun , Fan Xuemei , Jiang Yongxin 
Published: May 2023

Abstract
Background: Solute carrier family 25 member 24 (SLC25A24) is a member of the mitochondrial solute vector (MSC) protein superfamily. More and more evidence suggested that SLC family members play an extremely important role in cancers. However, the biological function of SLC25A24 in colorectal cancer has not been reported.
Methods: TCGA, GEO, UALCAN, Sangebox3.0 and TIDE databases were used to analyze SLC25A24 in colorectal cancer. The expression of SLC25A24 in 83 pairs of colorectal cancer tissues was detected by immunohistochemistry. qRT-PCR, Western blotting and apoptosis assays were used to explore the biological function of SLC25A24 in colorectal cancer.
Results: Through analysis of multiple databases, we found that SLC25A24 expression was higher in colorectal cancer than in adjacent normal tissues, and higher expression of SLC25A24 had a better prognosis. This was verified by clinical case analysis. In addition, based on multiple algorithms of immune infiltration, we found that SLC25A24 was significantly associated with immune infiltration in colorectal cancer. SLC25A24 was significantly associated with clinicopathological features in 83 patients with colorectal cancer. Importantly, SLC25A24 knockdown significantly promoted the apoptosis ability of colorectal cancer cells. In addition, we also found that lower expression of SLC25A24 was associated with poor prognosis and low immunotherapy sensitivity in patients with colorectal cancer. Therefore, SLC25A24 might be a biomarker for the treatment of colorectal cancer.
Conclusion: In summary, we found that SLC25A24 was higher expression in colorectal cancer than in adjacent normal tissues, and higher expression of SLC25A24 had a better prognosis. Importantly, we found that SLC25A24 inhibited apoptosis of colorectal cancer cells. In addition, SLC25A24 was associated with immune infiltration of colorectal cancer. Patients with lower expression of SLC25A24 were more prone to immune escape, while patients with higher expression of SLC25A24 were more conducive to immunotherapy. These results suggested that SLC25A24 might be a potential therapeutic target for patients with colorectal cancer.
The Role of Scoliosis on the Comorbidity and Demographics of Neurofibromatosis Type-1 Patients: A Retrospective Analysis of the National Inpatient Sample Database
Authors: Kodali Nilesh , Kumar Keshav D. , Schwartz Robert A. 
Published: May 2023

Abstract
Background
Neurofibromatosis type 1 (NF1) is the most common neurocutaneous syndrome in the United States, affecting every 1 in 3000 individuals. NF1 occurs due to non-functional mutations in the NF1 gene, which expresses neurofibromin, a protein involved in tumor suppression. As a result, NF1 typically presents with non-cancerous neoplasm masses called neurofibromas across the body. The most common musculoskeletal abnormality seen in around two-third of NF1 patients is scoliosis, an improver curvature of the spine. However, there is a lack of research on the effects of scoliosis on demographics and morbidities of NF1 patients.
Objective
We performed a national analysis to investigate the complex relationship between NF1 and scoliosis on patients’ demographics and comorbidities.
Method
We conducted a retrospective cross-sectional analysis of the 2017 US National Inpatient Sample database using univariable Chi-square analysis and multivariable binary logistic regression analysis to determine the interplay of NF1 and scoliosis on patients’ demographics and comorbidities.
Results
Our query resulted in 4,635 total NF1 patients, of which 475 (10.25%) had scoliosis and 4,160 (89.75%) did not. Demographic analysis showed that NF1 patients with scoliosis were typically younger, female, and white than when compared to NF1 patients without scoliosis. Comorbidity analysis showed that NF1 patients with scoliosis were more likely to develop malignant brain neoplasms, epilepsy, hydrocephalus, pigmentation disorders, hypothyroidism, diabetes with chronic complications, and coagulopathy disorders. NF1 patients with scoliosis were less likely to develop congestive heart failure, pulmonary circulation disease, peripheral vascular disease, paralysis, chronic pulmonary disease, lymphoma, and psychosis.
Conclusion
NF1 patients with scoliosis were predominantly younger, female, white patients. The presence of scoliosis in NF1 patients increases the risks for certain brain neoplasms and disorders, but serves a protective effect against some pulmonary and cardiac complications.
Anti-corrosion Properties of Functionalized Organo-silane coupling agents on Interstitial Free (IF) Steel in Simulated Saline solution
Authors: Badaik Sukanta , Ghosh Rishav , Ray Mausumi , Nidhi Monika , Bhagat A N , Ambade Balram , Rout T K 
Published: May 2023

Abstract
The selection of precursors for silane-based anti-corrosive coatings to protect steel is challenging to obtain in a few experiments as the protection performance varies with types of precursor molecule and surface of metal substrates. The coating performance of precursors was predicted through quantum chemical calculations (Density Functional Theory (DFT) where DFT parameters such as hardness (ƞ), dipole moment (µ), and the number of electrons transferred (∆N) to metal substrate enabling us to choose suitable silane coupling agents. DFT study and experimental validation reveals that precursor containing functional groups like -NH2, -SH, and -O- offers better protection against corrosion to galvanized IF steel compared to those without functional moiety. The order of corrosion inhibition is MPTMS (1M) ≈ APTES (1A) > GPTMS (1G) > TEOS(1T).
Comparative Evaluation of Antioxidant Activities in Mycelia, Fruiting Bodies and Spent Mushroom Substrate of Edible Mushrooms
Authors: Lavega Rebeca , Grifoll Vanessa , Siles María de las Nieves , Bravo Paula , Santoyo Susana , Pérez-Clavijo Margarita 
Published: May 2023

Abstract
Antioxidant activities of 13 commonly cultivated mushrooms in Spain were evaluated using total phenolic content (TPC), DPPH free radical scavenging (DPPH) assay, ABTS free radical scavenging (ABTS) assay, ferric reducing antioxidant power (FRAP) assay and cellular antioxidant activity (CAA) in Caco-2 cell line. Within a mushroom fruiting bodies, Agaricus bisporus var subrufescens possessed the highest TPC and antioxidant capacity in both chemical and cellular assays. Furthermore, the mycelia extracts with the best antioxidant potential in chemical assays were A. bisporus (white), A. bisporus var brunnescens (Portobello) and Hericium erinaceus. Caco-2 treatment with mycelia extracts revealed the absence of activity of any extract in the conditions assayed. In addition, the spent mushroom substrate from Agrocybe aegerita, H. erinaceus and Pholiota nameko are those with the highest polyphenol content and antioxidant capacity both by chemical methods and in vitro models. Results showed that there is no correlation between the polyphenols content, the chemical antioxidant assays and the corresponding values obtained in cellular models. Nevertheless, this study contributes to the rise of data relatively to the species consumed as fresh mushrooms and the possibility of their consumption as a source of antioxidant food.