Immune checkpoint inhibitors efficacy across solid cancers and the utility of PD-L1 as a biomarker of response: a systematic review and meta-analysis
Authors: Fitzsimmons T , Singh N , Walker TDJ , Newton C , Evans DGR , Crosbie EJ , Ryan NAJ 
Published: January 2023

Abstract
Background:
Immune checkpoint inhibitors (ICPI) are a tumour agnostic treatment. However, trials of their use have been site specific. Here we summarise the trial data and explore the utility of programmed death-ligand 1 (PD-L1) expression as a biomarker to direct their pan-cancer use.
Method:
A systematic review of literature, following PRISMA guidelines, was performed. Medline, Embase, Cochrane CENTRAL, NHS Health and Technology, and Web of Science were searched from their conception to June 2022 limited to the English language. The search terms and method were devised by a specialist medical librarian. Studies were limited to adults with solid cancers (excluding melanomas) treated with ICPIs. Only phase III randomised control trials (RCT) were included. The primary outcome was overall survival and secondary outcomes were progression free survival, PD-L1 expression, quality of life outcomes and adverse event data. Where present in eligible clinical trials, hazard ratios (HR), risk ratios (RR), standard error (SE) and 95% confidence intervals (CI) were extracted or calculated. Heterogeneity across studies was described with the use of an I2 score (Low: 25%, 50%: moderate, 75% low heterogeneity). HR pools inverse variance methods were adopted by Random Effects (RE). Means were standardised across any heterogenous scale limits.
Results:
In total 46,510 participants were included in the meta-analysis. Overall, meta-analysis favoured the use of ICPIs with an overall survival (OS) HR of 0.74 (95% CI 0.71 to 0.78). Lung cancers showed the most benefit in OS (HR 0.72 (95% 0.66 to 0.78)) followed by head and neck cancers (HR 0.75 (95% CI 0.66 to 0.84)) and gastro-oesophageal cancers (HR 0.75 (95% CI 0.61 to 0.92)). ICPIs seem to be efficacious at both primary presentation and recurrence (OS HR 0.73 (95% CI 0.68 to 0.77)) vs (OS HR 0.79 (95% CI 0.72 to 0.87)) respectively. Interestingly, subgroup analysis comparing studies in which most cancers demonstrated PD-L1 expression vs those studies in which a minority of cancer demonstrated PD-L1 expression reported similar effect of ICIP use on OS; oddly the data favoured ICPI use in studies with a minority of PD-L1 expression. Specifically, studies with minority PD-L1 expression had an HR 0.73 (95% CI 0.68 to 0.78) vs studies with majority PD-L1 expression HR 0.76 (95% CI 0.70 to 0.84). This was maintained even when studies exploring the same cancer site were directly compared. Subgroup analysis was performed comparing the impact on OS subdivided by the specific ICPI used. Where meta-analysis was performed, Nivolumab led to the greatest impact (HR 0.70 (95% CI 0.64 to 0.77)) with Avelumab failing to reach significance (HR 0.93 (95% CI 0.80 to 1.06)). However, overall heterogenicity was high (I2 = 95%). Finally, the use of ICPIs led to an improved side effect profile when compared with standard chemotherapy (RR 0.85 (95% CI 0.73 to 0.98)).
Conclusion:
ICPIs improve survival outcomes in cancer types. These effects are seen in the primary, recurrent, chemotherapy sensitive, chemotherapy resistant disease. These data support their use as a tumour agnostic therapy. Furthermore, they are well tolerated. However, PD-L1 as a biomarker for the targeting of ICPI use seems problematic. Other biomarkers such as mismatch repair or tumour mutational burden should be explored in randomised trials. In addition, there are still limited trials looking at ICPI use outside of lung cancer.