bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between maternal intra-abdominal pressure, pregnancy induced hypertension
Authors: Jayasundara Sajith , Goonewardene Malik , Dassanayake Lanka 
Published: March 2023

AbstractIntroductionPregnancy leads to a state of chronically increased intra-abdominal pressure (IAP) caused by a growing fetus, fluid, and tissue. Increased intra-abdominal pressure is leading to state of Intra-Abdominal Hypertension (IAH) and Abdominal Compartment Syndrome. Clinical features and risk factors of preeclampsia is comparable to abdominal compartment syndrome. Intra-abdominal pressure (IAP) may be associated with the pathogenesis of pregnancy induced hypertension.ObjectivesThe study aimed to determine the antepartum and postpartum IAP levels in women undergoing caesarean delivery (CD) and association between hypertension in pregnancy, and antepartum and postpartum IAP levels in women undergoing caesarean delivery (CD).MethodSeventy pregnant women (55 normotensive, 15 hypertension in pregnancy) undergoing antepartum, non-emergency CD, had their intravesical pressure measured before and after the CD, the intravesical pressure measurements obtained with the patient in the supine position were considered to correspond to the IAP. Multivariable linear regression models were used to study associations between intraabdominal pressure and baseline characteristics in normotensive pregnancies and hypertensive pregnancies.ResultsIn normotensive pregnancies at mean gestation age of 38 weeks +2days (95%CI 37+6 to 38 +4), mean antepartum IAP was 12.7 mmHg(95%CI 11.6 to 13.8) and the mean postpartum IAP was 7.3 mmHg (95% CI 11.6 to 13.8).Multivariable linear regression models showed hypertension in pregnancy group antepartum IAP positively associated with coefficient value of 1.617 (p= 0.268) comparing with normotensive pregnancy group.Postpartum IAP in hypertension in pregnancy group positively associated with coefficient value of 2.519 (p= 0.018) comparing with normotensive pregnancy group.IAP difference is negatively associated with hypertension in pregnancy (coefficient -1.013, p= 0.179)ConclusionIn normotensive pregnancies at term, the IAP was in the IAH range of the non-pregnant population. Higher Antepartum IAP and Postpartum IAP are associated with hypertension in pregnancy. Reduction of IAP from antepartum period to postpartum period was less with hypertension in pregnancy.
Analysis of the Health Economics Portfolio Funded by the National Institutes of Health in Response to Published Guidance
Authors: Burgdorf Caitlin E. , Riley William T. , Akbar Farheen , Zuehlke Abbey D. , Bibb Katrina , Eshleman Heather D. , Shah Ami , Belis Deshiree , Spittel Michael , Fearon Paula 
Published: March 2023

AbstractHealth economics is one of the interdisciplinary sciences that the National Institutes of Health (NIH) supports in order to pursue its overall mission to improve health. In 2015, NIH guidance was published to clarify the type of health economics research that NIH would continue to fund. This analysis aimed to determine if there were changes in the number of health economics applications received and funded by NIH after the release of the guidance. Health economics applications submitted to NIH both before and after the guidance was released were identified using a machine learning approach with input from subject matter experts. Application and funding trends were examined by fiscal year, method of application (solicited vs. unsolicited), and activity code. This study found that application and funding rates of health economics research was decreasing prior to guidance. Following publication of this guidance, the application and funding rate of health economics applications increased.
WITHDRAWN: Not To Skip Yet: Impact of Intravenous Thrombolysis on Final Infarction Volume and Clinical Outcomes in Early Arrivals Acute Large Vessel Occlusion: A Retrospective Cohort Study
Authors: Mohamed Ghada A , Essibayi Muhammed Amir , Sharf Valerie , Ali Hamid , Myeralis Pavlos , Lee William , Fugatt Robert , Jacks Kevin , Aboul-Nour Hassan , Spampinato M Vittoria , Wabnitz Ashley M , Holmstedt Christine 
Published: March 2023

Withdrawal StatementThe authors have withdrawn their manuscript because it was posted without the author’s permission and encompasses non-finalized data analysis and conclusions. Therefore, the authors do not wish this work to be cited as a reference for the project. If you have any questions, please contact the corresponding author.
Transcriptional profiling of populations in the clamRuditapes decussatussuggests genetically determined differentiation in gene-expression along parallel temperature gradients and between races of the Atlantic Ocean and West Mediterranean sea
Authors: Saavedra Carlos , Milan Massimo , Leite Ricardo , Cordero David , Patarnello Tomaso , Cancela Leonor , Bargelloni Luca 
Published: March 2023

AbstractOngoing ocean warming due to climate change poses new challenges for marine life and its exploitation. We have used transcriptomics to find genetically-based responses to increased temperature in natural populations of the marine clamRuditapes decussatusliving along parallel thermal gradients in southern Europe. Clams of the Atlantic and West Mediterranean races were collected in a northern (cool) and a southern (warm) localities. The animals were kept in running seawater in the warm, Atlantic southern locality for a 15-week period, during which water temperature raised to typical southern European summer values. After this period, the expression profile was obtained for a total of 34 clams and 11,025 probes by means of an oligonucleotide microarray. We found distinct transcriptional patterns for each population based on a total of 553 differentially expressed genes (DEG), indicating innate differences which probably have a genetic basis. Race and latitude contributed significantly to gene expression differences, with very different sets of DEG. A gene ontology analysis showed that races differed mainly at genes involved in ribosomal function and protein biosynthesis, while genes related to glutathione metabolism and ATP synthesis in the mitochondria were the most outstanding as to north/south transcriptional differences.
Cultured Mesenchymal Cells from Nasal Turbinate as a Cellular Model of the Neurodevelopmental Component of Schizophrenia Etiology
Authors: Tung Victoria Sook Keng , Mathews Fasil , Boruk Marina , Suppa Gabrielle , Foronjy Robert , Pato Michele , Pato Carlos , Knowles James A. , Evgrafov Oleg V. 
Published: March 2023

AbstractStudy of the neurodevelopmental molecular mechanisms of schizophrenia requires the development of adequate biological models such as patient-derived cells and their derivatives. We previously used cell lines with neural progenitor properties (CNON) derived from superior or middle turbinates of patients with schizophrenia and control groups to study gene expression specific to schizophrenia.In this study, we compared single cell-RNA seq data from two CNON cell lines, one derived from an individual with schizophrenia (SCZ) and the other from a control group, with two biopsy samples from the middle turbinate (MT), also from an individual with SCZ and a control. In addition, we compared our data with previously published data from olfactory neuroepithelium (1). Our data demonstrated that CNON originated from a single cell type which is present both in middle turbinate and olfactory neuroepithelium. CNON express multiple markers of mesenchymal cells. In order to define relatedness of CNON to the developing human brain, we also compared CNON datasets with scRNA-seq data of embryonic brain (2) and found that the expression profile of CNON very closely matched one of the cell types in the embryonic brain. Finally, we evaluated differences between SCZ and control samples to assess usability and potential benefits of using single cell RNA-seq of CNON to study etiology of schizophrenia.
Radiosynthesis automation, non-human primate biodistribution and dosimetry of K+channel tracer [11C]3MeO4AP
Authors: Zhou Yu-Peng , Wilks Moses Q. , Dhaynaut Maeva , Guehl Nicolas J. , Moon Sung-Hyun , Fakhri Georges El , Normandin Marc D. , Brugarolas Pedro 
Published: March 2023

ABSTRACTPurpose4-Aminopyridine (4AP) is a medication for the symptomatic treatment of multiple sclerosis. Several 4AP-based PET tracers have been developed for imaging demyelination. In preclinical studies, [11C]3MeO4AP has shown promise due to its high brain permeability, high metabolic stability, high plasma availability, and highin vivobinding affinity. To prepare for the translation to human studies, we developed a cGMP-compliant automated radiosynthesis protocol and evaluated the whole-body biodistribution and radiation dosimetry of [11C]3MeO4AP in non-human primates (NHPs).MethodsAutomated radiosynthesis was carried out using a GE TRACERlab FX-C Pro synthesis module. One male and one female adult rhesus macaques were used in the study. A high-resolution CT from cranial vertex to knee was acquired. PET data were collected using a dynamic acquisition protocol with 4 bed positions and 13 passes over a total scan time of ∼150 minutes. Based on the CT and PET images, volumes of interest (VOIs) were manually drawn for selected organs. Non-decay corrected time-activity curves (TACs) were extracted for each VOI. Radiation dosimetry and effective dose were calculated from the integrated TACs using OLINDA software.ResultsFully automated radiosynthesis of [11C]3MeO4AP was achieved with 7.3 ± 1.2 % (n = 4) of non-decay corrected radiochemical yield within 38 min of synthesis and purification time. [11C]3MeO4AP distributed quickly throughout the body and into the brain. The organs with highest dose were the kidneys. The average effective dose of [11C]3MeO4AP was 4.27 ± 0.57 μSv/MBq. No significant changes in vital signs were observed during the scan.ConclusionThe cGMP compliant automated radiosynthesis of [11C]3MeO4AP was developed. The whole-body biodistribution and radiation dosimetry of [11C]3MeO4AP was successfully evaluated in NHPs. [11C]3MeO4AP shows lower average effective dose than [18F]3F4AP and similar average effective dose as other carbon-11 tracers.
A reference assembly for the legume cover crop, hairy vetch (Vicia villosa)
Authors: Fuller Tyson , Koch Lisa M. , Kucek Lisa Kissing , Ali Shahjahan , Mangelson Hayley , Hernandez Timothy , Smith Timothy P.L. , Bickhart Derek M. , Riday Heathcliffe , Sullivan Michael L. 
Published: March 2023

ABSTRACTVicia villosais an incompletely domesticated annual legume of the Fabaceae family native to Europe and Western Asia.V. villosais widely used as a cover crop and as a forage due to its ability to withstand harsh winters. A reference-quality genome assembly (Vvill1.0) was prepared from low error rate long sequence reads to improve genetic-based trait selection of this species. The Vvill1.0 assembly includes seven scaffolds corresponding to the seven estimated linkage groups and comprising approximately 68% of the total genome size of 2.03 gigabase pairs (Gbp). This assembly is expected to be a useful resource for genetic improvement of this emerging cover crop species as well as to provide useful insights into plant genome evolution.
Deep Learning Initialized Compressed Sensing (Deli-CS) in Volumetric Spatio-Temporal Subspace Reconstruction
Authors: Iyer Siddharth S. , Schauman S. Sophie , Sandino Christopher M. , Yurt Mahmut , Cao Xiaozhi , Liao Congyu , Ruengchaijatuporn Natthanan , Chatnuntawech Itthi , Tong Elizabeth , Setsompop Kawin 
Published: March 2023

AbstractIntroductionSpatio-temporal MRI methods enable whole-brain multi-parametric mapping at ultra-fast acquisition times through efficient k-space encoding, but can have very long reconstruction times, which limit their integration into clinical practice. Deep learning (DL) is a promising approach to accelerate reconstruction, but can be computationally intensive to train and deploy due to the large dimensionality of spatio-temporal MRI. DL methods also need large training data sets and can produce results that don’t match the acquired data if data consistency is not enforced. The aim of this project is to reduce reconstruction time using DL whilst simultaneously limiting the risk of deep learning induced hallucinations, all with modest hardware requirements.MethodsDeep Learning Initialized Compressed Sensing (Deli-CS) is proposed to reduce the reconstruction time of iterative reconstructions by “kick-starting” the iterative reconstruction with a DL generated starting point. The proposed framework is applied to volumetric multi-axis spiral projection MRF that achieves whole-brain T1 and T2 mapping at 1-mm isotropic resolution for a 2-minute acquisition. First, the traditional reconstruction is optimized from over two hours to less than 40 minutes while using more than 90% less RAM and only 4.7 GB GPU memory, by using a memory-efficient GPU implementation. The Deli-CS framework is then implemented and evaluated against the above reconstruction.ResultsDeli-CS achieves comparable reconstruction quality with 50% fewer iterations bringing the full reconstruction time to 20 minutes.ConclusionDeli-CS reduces the reconstruction time of subspace reconstruction of volumetric spatio-temporal acquisitions by providing a warm start to the iterative reconstruction algorithm.
Changes in local interaction rules during ontogeny underlie the evolution of collective behavior
Authors: Paz Alexandra , Holt Karla J. , Clarke Anik , Aviles Ari , Abraham Briana , Keene Alex C. , Duboué Erik R. , Fily Yaouen , Kowalko Johanna E. 
Published: March 2023

AbstractCollective motion emerges from individual interactions which produce groupwide patterns in behavior. While adaptive changes to collective motion are observed across animal species, how local interactions change when these collective behaviors evolve is poorly understood. Here, we use the Mexican tetra,A. mexicanus,which exists as a schooling surface form and a non-schooling cave form, to study differences in how fish alter their swimming in response to neighbors across ontogeny and between evolutionarily diverged populations. We find that surface fish undergo a transition to schooling during development that occurs through increases in inter-individual alignment and attraction mediated by changes in the way fish modulate speed and turning relative to neighbors. Cavefish, which have evolved loss of schooling, exhibit neither of these schooling-promoting interactions at any stage of development. These results reveal how evolution alters local interaction rules to produce striking differences in collective behavior.
A 20th-centuryCannabispollen increase in a Pyrenean varved record and its potential causes
Authors: Rull Valentí , Vegas-Vilarrúbia Teresa 
Published: March 2023

AbstractThe continuous, varved and absolutely dated sedimentary record of Lake Montcortès (Iberian Pyrenees) has provided evidence for a distinct and characteristic 20thcentury (1980s) increase inCannabispollen (20C) that persists today. This event was coeval with the geographical shift of the hemp production center in the Iberian Peninsula from east to northeast (where Lake Montcortès lies), which was accompanied by a significant production increase. This increasing trend was fostered by the renewed interest of the paper industry in hemp and was promoted by the onset of European Union subsidies to hemp cultivation. Illegal cannabis crops could have also contributed to theCannabispollen increase, but sound evidence is still lacking. These preliminary conclusions should be reinforced by increasing the resolution of the current palynological record and modeling the dispersal ofCannabispollen around the Montcortès region. More similar high-resolution records are needed to verify the geographical extent of the 20C event. Additionally, Lake Montcortès varved sediments are proposed as a suitable candidate to characterize the onset of the “Anthropocene” epoch (mid-20thcentury), as currently defined by the Anthropocene Working Group.
Structural insights into ligand recognition and selectivity of the human hydroxycarboxylic acid receptor HCAR2
Authors: Pan Xin , Ye Fang , Ning Peiruo , Zhang Zhiyi , Zhang Binghao , Chen Geng , Gao Wei , Qiu Chen , Wu Zhangsong , Gong Kaizheng , Li Jiancheng , Xia Jiang , Du Yang 
Published: March 2023

AbstractHydroxycarboxylic acid receptor 2 (HCAR2) belongs to the family of class A G-protein-coupled receptors with key roles in regulating lipolysis and free fatty acid formation in humans. It is deeply involved in many pathophysiological processes and serves as an attractive target for the treatment of neoplastic, autoimmune, neurodegenerative, inflammatory, and metabolic diseases. Here, we report four cryo-EM structures of human HCAR2-Gi1 complexes with or without agonists, including the drugs niacin and acipimox, and the highly subtype-specific agonist MK-6892. Combined with molecular docking and functional analysis, we have revealed the recognition mechanism of HCAR2 for different agonists and summarized the general pharmacophore features of HCAR2 agonists, which are based on three key residues R1113.36, S17945.52, and Y2847.43. Notably, the MK-6892-HCAR2 structure shows an extended binding pocket relative to other agonist-bound HCAR2 complexes. In addition, the key residues that determine the ligand selectivity between the HCAR2 and HCAR3 are also illuminated. Our findings provide structural insights into the ligand recognition, selectivity, activation, and G protein coupling mechanism of HCAR2, which sheds light on the design of new HCAR2-targeting drugs for greater efficacy, higher selectivity, and fewer or no side effects.
Accumulation of m6A exhibits stronger correlation with MAPT than β-amyloid pathology in an APPNL-G-F/MAPTP301Smouse model of Alzheimer’s disease
Authors: Jiang Lulu , Roberts Rebecca , Wong Melissa , Zhang Lushuang , Webber Chelsea Joy , Kilci Alper , Jenkins Matthew , Sun Guangxin , Rashad Sherif , Sun Jingjing , Dedon Peter C , Daley Sarah Anne , Xia Weiming , Ortiz Alejandro Rondón , Dorrian Luke , Saito Takashi , Saido Takaomi C. , Wolozin Benjamin 
Published: March 2023

AbstractThe study for the pathophysiology study of Alzheimer’s disease (AD) has been hampered by lack animal models that recapitulate the major AD pathologies, including extracellular β-amyloid (Aβ) deposition, intracellular aggregation of microtubule associated protein tau (MAPT), inflammation and neurodegeneration. We now report on a double transgenic APPNL-G-FMAPTP301Smouse that at 6 months of age exhibits robust Aβ plaque accumulation, intense MAPT pathology, strong inflammation and extensive neurodegeneration. The presence of Aβ pathology potentiated the other major pathologies, including MAPT pathology, inflammation and neurodegeneration. However, MAPT pathology neither changed levels of amyloid precursor protein nor potentiated Aβ accumulation. The APPNL-G-F/MAPTP301Smouse model also showed strong accumulation of N6-methyladenosine (m6A), which was recently shown to be elevated in the AD brain. M6A primarily accumulated in neuronal soma, but also co-localized with a subset of astrocytes and microglia. The accumulation of m6A corresponded with increases in METTL3 and decreases in ALKBH5, which are enzymes that add or remove m6A from mRNA, respectively. Thus, the APPNL-G-F/MAPTP301Smouse recapitulates many features of AD pathology beginning at 6 months of aging.
Active-feedback 3D single-molecule tracking using a fast-responding galvo scanning mirror
Authors: Tan Xiaochen , Hou Shangguo , Zhang Chen , Niver Anastasia , Johnson Alexis , Welsher Kevin D. 
Published: March 2023

AbstractReal-time three-dimensional single-particle tracking (RT-3D-SPT) allows continuous detection of individual freely diffusing objects with high spatiotemporal precision by applying closed-loop active feedback in an optical microscope. However, the current tracking speed in RT-3D-SPT is primarily limited by the response time of control actuators, impeding long-term observation of fast diffusive objects such as single molecules. Here, we present an RT-3D-SPT system with improved tracking performance by replacing the XY piezoelectric stage with a galvo scanning mirror with an approximately five-time faster response rate (~5 kHz). Based on the previously developed 3D single-molecule active real-time tracking (3D-SMART), this new implementation with a fast-responding galvo mirror eliminates the mechanical movement of the sample and allows more rapid response to particle motion. The improved tracking performance of the galvo mirror-based implementation is verified through simulation and proof-of-principle experiments. Fluorescent nanoparticles and ~ 1 kB double-stranded DNA molecules were tracked via both the original piezoelectric stage and new galvo mirror implementations. With the new galvo-based implementation, notable increases in tracking duration, localization precision, and the degree to which the objects are locked to the center of the detection volume were observed. These results suggest faster control response elements can expand RT-3D-SPT to a broader range of chemical and biological systems.
Real time analysis of SARS-CoV-2 induced cytolysis reveals distinct variant-specific replication profiles
Authors: Scheuermann Sarah E , Goff Kelly , Rowe Lori A , Beddingfield Brandon J , Maness Nicholas J 
Published: March 2023

AbstractThe continuous evolution of new SARS-CoV-2 variants with enhanced immune evasion capacity suggests the entire population is and will continue to be potentially vulnerable to infection despite pre-existing immunity. The ability of each new variant to evade host humoral immunity is the focus of intense research across the globe. Each variant may also harbor unique replication capabilities relevant for disease and transmission. Here we demonstrate the utility of a new approach to assessing viral replication kinetics using Real Time Cell Analysis (RTCA). Virus induced cell death is measured in real time by the detection of electrical impedance through cell monolayers. Using this system, we quantified replication kinetics of five clinically important viral variants; USA WA1/2020 (an A1 ancestral lineage isolate), Delta, and Omicron subvariants BA.1, BA.4, and BA.5. We identified multiple kinetic measures that proved useful in variant replication comparisons including time (in hours) to the maximum rate of cell death at each log10 viral dilution and the slope at the maximum rate of cell death. We found that WA1/2020 and Delta were the most rapid but in distinct ways. While WA1/2020 induced cell death most rapidly after inoculation, Delta was slightly slower to reach cell death, it appeared to kill cells faster once cytotoxic effects began. Interestingly, BA.1, showed substantially reduced replication kinetics relative to all other variants. Together, these data show that real time analysis of cell death is a robust method to assess replicative capacity of any given SARS-CoV-2 variant rapidly and quantitatively, which may be useful in assessment of newly emerging variants.