bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the role of endoplasmic reticulum stress genes in endometrial cancer and their association with tumor immunity
Authors: Zhang Tang ansu , Zhang Qian , Zhang Jun , Zhao Rong , Shi Rui , Wei Sitian , Liu Shuangge , Zhang Qi , Wang Hongbo 
Published: April 2023

Abstract
Background: Endometrial cancer (EC) is one of the worldwide gynecological malignancies. Endoplasmic reticulum (ER) stress is the cellular homeostasis disturbance that participates in cancer progression. However, the mechanisms of ER stress on EC have not been fully elucidated.
Method: The ER Stress-related genes were obtained from Gene Set Enrichment Analysis (GSEA) and GeneCards, and the RNA-seq and clinical data were downloaded from The Cancer Genome Atlas (TCGA). The risk signature was constructed by the Cox regression and the least absolute shrinkage and selection operator (LASSO) analysis. The significance of the risk signature and clinical factors were tested by time-dependent receiver operating characteristic (ROC) curves, and the selected were to build a nomogram. The immunity correlation was particularly analyzed, including the related immune cells, pathways, and immune checkpoints. Functional enrichment, potential chemotherapies, and in vitro validation were also conducted.
Result: An ER Stress-based risk signature, consisting of TRIB3, CREB3L3, XBP1, and PPP1R15A was established. Patients were randomly divided into training and testing groups with 1:1 ratio for subsequent calculation and validation. Based on risk scores, high- and low-risk subgroups were classified, and low-risk subgroup demonstrated better prognosis. The Area Under Curve (AUC) demonstrated a reliable predictive capability of the risk signature. The majority of significantly different immune cells and pathways were enriched more in low-risk subgroup. Similarly, several typical immune checkpoints, expressed higher in low-risk subgroup. Patients of the two subgroups responded differently to chemotherapies.
Conclusion: We established an ER Stress-based risk signature that could effectively predict EC patients' prognosis and their immune correlation.
Genetic diversity and antagonistic properties of Trichoderma strains from the crop rhizospheres in southern Rajasthan, India
Authors: Jambhulkar Prashant P. , Singh Bhumica , Raja M. , Ismaiel Adnan , Lakshman Dilip K. , Tomar Maharishi , Sharma Pratibha 
Published: April 2023

Abstract

There are fewer studies on Trichoderma diversity in agricultural fields. The rhizosphere of 16 crops was analyzed for Trichoderma species in 7 districts of Rajasthan state of India. Based on DNA sequence of translation elongation factor 1α (tef-1α), and morphological characteristics, 60 isolates were identified as 11 species: Trichoderma brevicompactum, species in Harzianum clade identified as T. afroharzianum, T. inhamatum, T. lentiforme, T. camerunense, T. asperellum, T. asperelloides, T. erinaceum, T. atroviride, T. ghanense, and T. longibrachiatum. T. brevicompactum is the most commonly occurring strain followed by T. afroharzianum. No new species were described in this study. T. lentiforme, showed its first occurrence outside the South American continent. The morphological and cultural characteristics of the major species were observed, described, and illustrated in detail. The isolates were tested for their antagonistic effect against three soilborne plant pathogens fungi: Sclerotium rolfsii, Rhizoctonia solani, and Fusarium verticillioides in plate culture assays. One of the most potent strains was T. afroharzianum BThr29 having a maximum in vitro inhibition of S. rolfsii (76.6%), R. solani (84.8%), and F. verticillioides (85.7%). Such information on crop selectivity, antagonistic properties, and geographic distribution of Trichoderma species will be beneficial for developing efficient Trichoderma-based biocontrol agents.
“The most culturally safe training I’ve ever had.” The co-design of a culturally safe Managing hepatitis B training course with and for the Aboriginal health workforce of the Northern Territory of Australia
Authors: Hosking Kelly , De Santis Teresa , Vintour-Cesar Emily , Wilson Phillip Merrdi , Bunn Linda , Gurruwiwi George Garambaka , Wurrawilya Shiraline , Bukulatjpi Sarah Mariyalawuy , Nelson Sandra , Ross Cheryl , Binks Paula , Schroder Phoebe , Davis Joshua S , Taylor Sean , Connors Christine , Davies Jane 
Published: April 2023

Abstract
Background The Aboriginal health workforce provide responsive, culturally safe health care. We aimed to co-design a culturally safe course with and for the Aboriginal health workforce. We describe the factors which led to the successful co-design, delivery, and evaluation of the “Managing hepatitis B” course for the Aboriginal health workforce.Methods A Participatory Action Research approach was used, involving ongoing consultation to iteratively co-design and then develop course content, materials, and evaluation tools. An Aboriginal and Torres Strait Islander research and teaching team received education in chronic hepatitis B and teaching methodologies. Pilot courses were held, in remote communities of the Northern Territory, using two-way learning and teach-back methods to further develop the course and assess acceptability and learnings. Data collection involved focus group discussions, in-class observations, reflective analysis, and use of co-designed and assessed evaluation tools.Results Twenty-six participants attended the pilot courses. Aboriginal and Torres Strait Islander facilitators delivered a high proportion of the course. Evaluations demonstrated high course acceptability, cultural safety, and learnings. Key elements contributing to success and acceptability were acknowledging, respecting, and integrating cultural differences into education, delivering messaging and key concepts through an Aboriginal and Torres Strait Islander lens, using culturally appropriate approaches to learning including storytelling and visual teaching methodologies. Evaluation of culturally safe frameworks and findings from the co-design process led to the creation of a conceptual framework, underpinned by meeting people’s basic needs, and offering a safe and comfortable environment to enable productive learning with attention to the following: sustenance, financial security, cultural obligations, and gender and kinship relationships.Conclusions Co-designed education for the Aboriginal health workforce must embed principles of cultural safety and meaningful community consultation to enable an increase in knowledge and empowerment. The findings of this research can be used to guide the design of future health education for First Nations health professionals and to other non-dominant cultures. The course model has been successfully transferred to other health issues in the Northern Territory.
Tyrosine phosphorylation of band 3 impairs the storage quality of SRBCs in the Tibetan HAPC population
Authors: Wu xiaodong , Liu Zhijuan , Hao Doudou , Zhao Qin , Li Wanjing , Xie Maodi , Feng Xia , Liao Xia , Chen Siyuan , Wang Siyu , Zhou Chaohua , Long Wenchun , Zhong Yajun , Li Shen , Cao Ye , Wang Hong , Wang Aiping , Xu Yuehong , Huang Min , Liu Jiaxin , Zhong Rui , Wu Yunhong , He Zeng 
Published: April 2023

Abstract
Due to environmental hypoxia on the Tibetan Plateau, local residents often exhibit a compensative increase in hemoglobin concentration to maintain the body's oxygen supply. However, increases in hemoglobin and hematocrit (Hct) pose a serious challenge to the quality of stored suspended red blood cells (SRBCs) prepared from the blood of high-hemoglobin populations, especially populations at high altitude with polycythemia in Tibet. To explore the difference in storage quality of SRBCs prepared from plateau residents with a high hemoglobin concentration, blood donors were recruited from Tibet (> 3600 m) and Chengdu (≈ 500 m) and divided into a high-altitude control (HAC) group, high-altitude polycythemia (HAPC) group and lowland control (LLC) group according to hemoglobin concentration and altitude of residence. The extracellular acidification rate (ECAR), pyruvate kinase (PK) activity and band 3 tyrosine phosphorylation were analyzed on the day of blood collection. Then, whole-blood samples were processed into SRBCs, and storage quality parameters were analyzed aseptically on days 1, 14, 21 and 35 of storage. Overall, we found that tyrosine 21 phosphorylation activated glycolysis by releasing glycolytic enzymes from the cytosolic domain of band 3, thus increasing glucose consumption and lactate accumulation during storage, in the HAPC group. In addition, band 3 tyrosine phosphorylation impaired erythrocyte deformability, accompanied by the highest hemolysis rate in the HAPC group, during storage. We believe that these results will stimulate new ideas to further optimize current additive solutions for the high-hemoglobin population in Tibet and reveal new therapeutic targets for the treatment of HAPC populations.
Structural and Humidity Sensing Properties of Polypyrrole composites containing Li2O and ZnO nanoparticles
Authors: Madival Rajshekar L , M Prashantkumar , N Nagaraja , Kannur Vinay V , T Ravikiran Y 
Published: April 2023

Abstract
We report the structural properties and humidity sensing abilities of polypyrrole (PPy) composites containing ZnO nanoparticles (NPs) and Li2O. The PPy was synthesized following chemical oxidation method. The PPy50-ZnO(NPs) 50- Li2O x (where x = 0,2,4,6,8 & 10) composites were prepared by physical mixing using ball-milling method. The as-prepared composites were subjected to XRD, FTIR and EDAX. The structural analysis using these tools hinted at the presence of Li2O in the composites and the effect of variation of Li2O in the composites is emphasized. The humidity sensing ability of pristine PPy and the composites has been investigated, at room temperature, by measuring the change in electrical resistance of the sample due to the variation in relative humidity from 11–97% RH. The response to humidity and recovery times were determined. The mechanism of humidity sensing in these composites was understood by Grotthus theory. Among all the composites, the composite x = 10 (PZL10) has exhibited very good response and recovery time and has the potential to be used as a low-cost and highly effective humidity sensor.
Added value of the pulmonary vein pulsatility index and its correlation to neonatal umbilical artery pH in fetal growth restrictions: A Vietnamese matched cohort study
Authors: Pham Minh Son , Tran Dinh Vinh , Pham Chi Kong , Truong Thi Linh Giang , Nguyen Vu Quoc Huy 
Published: April 2023

Abstract
Background: In clinical obstetrics, many guidelines recommended the use of Doppler fetal ductus venosus blood flow to monitor and to manage fetal growth restriction (FGR). The ductus venosus and the pulmonary venous flow pattern of fetuses are similar. Umbilical artery pH (UA pH) is essential in identifying adverse pregnancy outcomes, particularly in fetal growth restriction cases. Nevertheless, the literature indicates that the relationship between pulmonary vein pulsatility index (PVPI) and UA pH in FGR cases has not been well investigated. This study aimed to identify the alteration in PVPI in FGR cases and evaluate the correlation between PVPI and UA pH in FGR newborns.
Methods: This matched cohort study of singleton pregnancies from 28+0 to 40+0 weeks of gestation without congenital abnormalities included 135 cases of FGR (disease group) and 135 cases of normal growth (control group). The PVPI was measured at the proximal segment of the right or left pulmonary vein, approximately 5 mm from the left atrium wall. The umbilical artery pulsatility index (UAPI) was measured on the free umbilical cord. An elective cesarean section or labor induction are both options for ending the pregnancy, depending on the condition of the mother or fetus. Umbilical artery blood samples were collected within 5 minutes of delivery for UA pH measurement. SPSS version 20 and Medcalc version 20.1 were used for data analysis.
Results: FGR cases had a significantly higher mean fetal PVPI than the control group (1.16 ± 0.26 vs. 0.84 ± 0.16; p < 0.01), and PVPI and UAPI were positively correlated (r = 0.63; p < 0.001). PVPI and UA pH were negatively correlated in FGR patients, with r = -0.68; p < 0.001. The PVPI value on the 95th percentile had a prognostic value of UA pH < 7.20 with a sensitivity of 88.2%, specificity of 66.3%, positive predictive value of 46.9%, and negative predictive value of 94.3%.
Conclusions: There was a statistically significant difference in PVPI values in FGR cases compared to the normal growth group, a positive correlation between PVPI and UAPI, and a negative correlation between PVPI and UA pH. PVPI might have a prognostic meaning in predicting UA pH at birth.
Cortical and Subcortical Structural Differences in Psychostimulant-Free ADHD Youth With and Without a Family History of Bipolar I Disorder: A Cross-Sectional Morphometric Comparison
Authors: Lei Du , Zhu Ziyu , Qin Kun , Tallman Maxwell , Patino L. Rodrigo , Fleck David , Gong Qiyong , Sweeney John , DelBello Melissa , McNamara Robert 
Published: April 2023

Abstract
Although attention-deficit/hyperactivity disorder (ADHD) and a family history of bipolar I disorder (BD) both increase the risk for developing BD, associated neuroanatomical substrates remain poorly understood. This study compared cortical and subcortical gray matter morphology in psychostimulant-free ADHD youth with and without a first-degree relative with BD and typically developing healthy controls. ADHD youth (ages 10–18 years) with (‘high-risk’, HR) or without (‘low-risk’, LR) a first-degree relative with BD and healthy comparison youth (HC) were enrolled. High-resolution 3D T1-weighted images were acquired using a Philips 3.0 T MR scanner. The FreeSurfer image analysis suite was used to measure cortical thickness, surface area, and subcortical volumes. A general linear model evaluated group differences with age and sex as covariates, and exploratory correlational analyses investigated associations with different symptom ratings. A total of n = 142 youth (mean age: 14.16 ± 2.54 years, 35.9% female) were included in the analysis (HC, n = 48; LR, n = 49; HR, n = 45). The HR group exhibited a more severe symptom profile, including higher mania and dysregulation scores, compared to the LR group. For subcortical volumes, the HR group exhibited smaller bilateral thalamic, hippocampal, and left caudate nucleus volumes compared to both LR and HC, and smaller right caudate nucleus compared with LR. No differences were found between LR and HC groups. For cortical surface area, HR exhibited lower parietal and temporal surface area compared with HC and LR, and lower orbitofrontal and superior frontal surface area compared to LR. LR exhibited greater right pars opercularis surface area compared with the HC group. A subset of these cortical regions correlated with relevant symptom ratings. These findings suggest that ADHD in conjunction with BD family history is associated with a more a severe symptom profile and a neuroanatomical phenotype that distinguishes it from ADHD without a BD family history.
Immuno-physiological effects of dietary beta-glucan on Rohu, Labeo rohita challenged with Aeromonas veronii
Authors: Saha Tutul Kumar , . Mariom , Rahman Tanvir , Moniruzzaman Mohammad , Min Taesun , Hossain Zakir 
Published: April 2023

Abstract
Beta-glucans have immense potential to stimulate immune modulation in fish by being injected intramuscularly, supplemented with feed or immersion routes of administration. Here, we looked at how supplementing the diet of Rohu carp, Labeo rohita, with beta-glucan, influenced the immune function. A beta-glucan-supplemented diet containing 10% mushroom powder was administered for 120 days. Afterward, analyses were conducted on different immunological parameters such as antioxidants, respiratory burst, reactive oxygen species (ROS), alternative complement activity, and serum immunoglobulin, which resulted significant increases (p < 0.05 and p < 0.01) for the beta-glucan fed immune primed L. rohita, indicating enhanced immunity. Additionally, analyzing various hematological parameters such as erythrocytes and leukocytes count were assessed using standardized methods to elucidate the immunomodulatory effects, indicating a positive effect of beta-glucan on overall fish health. Furthermore, the bacterial challenge test with a 1.92×104 CFU/ml intramuscular dose of Aeromonas veronii showed an enhanced disease-defending system as total serum protein and lysozyme activity levels accelerated significantly (p < 0.01). Nevertheless, beta-glucan ameliorated the stress indicating parameters like Acetylcholinesterase (AChE), serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT) enzyme activities results suggested the fish's physiology was unaffected. Therefore, it was determined that adding dietary beta-glucan supplementation could significantly boost up the immune responses in Rohu carp.
Osmo-respiratory compromise in the mosshead sculpin (Clinocottus globiceps): effects of temperature, hypoxia, and re-oxygenation on rates of diffusive water flux and oxygen uptake
Authors: Onukwufor John O. , Somo Derek A. , Richards Jeffrey G. , Wood Chris M. 
Published: April 2023

Abstract
In nature, mosshead sculpins (Clinocottus globiceps) are challenged by fluctuations in temperature and oxygen levels in their environment. However, it is unclear how mosshead sculpins modulate the permeability of their branchial epithelia to water and O2 in response to temperature or hypoxia stress. Acute decrease in temperature from 13 to 6 oC reduced diffusive water flux rate by 22% and ṀO2 by 51%, whereas acute increase in temperature from 13 to 25 oC increased diffusive water flux rate by 217% and ṀO2 by 140%, yielding overall Q10 values of 2.08 and 2.47 respectively. Acute reductions in oxygen tension from >95% to 20% or 10% air saturation did not impact diffusive water flux rates, however, ṀO2 was reduced significantly by 36% and 65% respectively. During 1-h or 3-h recovery periods diffusive water flux rates were depressed while ṀO2 exhibited overshoots beyond the normoxic control level. Many responses differed from those seen in our parallel earlier study on the tidepool sculpin, a cottid with similar hypoxia tolerance but much smaller gill area that occupies a similar environment. Overall, our data suggest that during temperature stress, diffusive water flux rates and ṀO2 follow the traditional osmo-respiratory compromise pattern, but during hypoxia and re-oxygenation stress, diffusive water flux rates are decoupled from ṀO2.
Association of marine PUFAs intakes with cardiovascular disease, all-cause mortality, and cardiovascular mortality in American adult male patients with dyslipidemia: The U.S. National Health and Nutrition Examination Survey, 2001 to 2016
Authors: Tang Xuanfeng , Lv Xinyi , Wang Ruohua , Li Xiaoqing , Xu Wenyu , Wang Nan , Ma Shuran , Huang He , Niu Yucun 
Published: April 2023

Abstract
Background Among the studies on the relationship between marine polyunsaturated fatty acids (PUFAs) and health, few studies have focused on docosapentaenoic acid (DPA) and cardiovascular health. And the health effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on cardiovascular disease (CVD) were not consistent.Objective The purpose of this study was to assess the relationship between different types of marine polyunsaturated fatty acids intakes and cardiovascular disease, all-cause mortality, and cardiovascular mortality in adult U.S. males with dyslipidemia.Methods Adult male with dyslipidemia in the study were screened from the National Health and Nutrition Examination Survey (NHANES) from 2001 to 2016. Death outcomes were determined by linking them to National Death Index (NDI) records through 2019. Weighted logistic regression models and Cox proportional hazards regression models were used in the study.Results In the fully adjusted models, participants with the highest tertile of dietary docosapentaenoic acid had lower risks of angina (OR = 0.53, 95%CI: 0.37–0.77), stroke (OR = 0.61, 95%CI: 0.42–0.87), all-cause death (HR = 0.79, 95%CI: 0.67–0.92) and CVD-specific death (HR = 0.74, 95%CI: 0.55–0.98) than those with the lowest tertile. The risks of cardiovascular disease, all-cause death, and cardiovascular disease-specific death among participants in the highest tertile of EPA and DHA were not significantly different from those in the lowest tertile (p > 0.05).Conclusions Cardiovascular disease risk, all-cause mortality, and CVD mortality were inversely associated with dietary DPA intake but not EPA and DHA intakes in U.S. male participants with dyslipidemia.
Association between concomitant proton pump inhibitor use and survival of patients with metastatic prostate cancer receiving abiraterone acetate: a post-hoc analysis of pooled data from three randomized controlled trials
Authors: Fukuokaya Wataru , Mori Keiichiro , Yanagisawa Takafumi , Akazawa Kohei , Shimomura Tatsuya , Kimura Takahiro 
Published: April 2023

Abstract
Background

Evidence suggests proton pump inhibitor (PPI) use may attenuate the effect of abiraterone acetate plus prednisone (AAP) in metastatic prostate cancer via the modification of gut microbiota. This study aimed to examine whether concomitant PPI use is associated with survival in patients with metastatic prostate cancer treated with androgen deprivation therapy (ADT) and AAP.

Methods

Post-hoc analysis was conducted in patients with metastatic castration-sensitive prostate cancer (mCSPC) and metastatic castration-resistant prostate cancer (mCRPC) treated in the LATITUDE, COU-AA-301, and COU-AA-302 trials (ADT vs. ADT plus AAP). PPI users and non-users were compared for restricted mean overall survival time (RMOST) and restricted mean progression-free survival time (RMPFST) based on inverse probability of treatment weight (IPTW)-adjusted Kaplan-Meier curves. IPTW-adjusted Cox regression models were used to assess heterogeneity of treatment effect.

Results

In patients treated with AAP, PPI use was associated with inferior RMOST [difference (95% confidence interval): -4.2 (-7.0 to -1.4)] and RMPFST [-3.5 (-6.6 to -0.4)] compared with non-users. However, RMOST and RMPFST were similar between PPI users and non-users in patients treated with ADT alone [RMOST, -2.6 (-5.8 to 0.6); RMPFST, -1.7 (-4.8 to 1.4)]. Interaction term analyses did not show evidence of heterogeneity in treatment effect between AAP and ADT, despite the prominent treatment effect shown in mCSPC vs. mCRPC.

Conclusions

PPI use may be associated with inferior survival in patients with metastatic prostate cancer who receive ADT plus AAP. Discontinuing unnecessary PPI use might improve those outcomes.