bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA methylation and miR‐92a‐3p‐mediated repression of HIP1R promotes pancreatic cancer progression by activating the PI3K/AKT pathway
Authors: Zhu Sixian , Xu Huiting , Chen Runzhi , Shen Qian , Yang Dongmei , Peng Hui , Tong Jin , Fu Qiang 
Published: February 2023

AbstractPancreatic cancer (PAAD) is a highly malignant tumour characterized of high mortality and poor prognosis. Huntingtin‐interacting protein 1‐related (HIP1R) has been recognized as a tumour suppressor in gastric cancer, while its biological function in PAAD remains to be elucidated. In this study, we reported the downregulation of HIP1R in PAAD tissues and cell lines, and the overexpression of HIP1R suppressed the proliferation, migration and invasion of PAAD cells, while silencing HIP1R showed the opposite effects. DNA methylation analysis revealed that the promoter region of HIP1R was heavily methylated in PAAD cell lines when compared to the normal pancreatic duct epithelial cells. A DNA methylation inhibitor 5‐AZA increased the expression of HIP1R in PAAD cells. 5‐AZA treatment also inhibited the proliferation, migration and invasion, and induced apoptosis in PAAD cell lines, which could be attenuated by HIP1R silencing. We further demonstrated that HIP1R was negatively regulated by miR‐92a‐3p, which modulates the malignant phenotype of PAAD cells in vitro and the tumorigenesis in vivo. The miR‐92a‐3p/HIP1R axis could regulate PI3K/AKT pathway in PAAD cells. Taken together, our data suggest that targeting DNA methylation and miR‐92a‐3p‐mediated repression of HIP1R could serve as novel therapeutic strategies for PAAD treatment.
Proportion of subsequent clinic visits among persons without regular clinic visits who were screened as having hyperglycemia: A retrospective cohort study
Authors: Matsumura Hiroshi , Sugiyama Takahiro , Sakata Nobuo , Ihana‐Sugiyama Noriko , Imai Kenjiro , Ohsugi Mitsuru , Ueki Kohjiro , Tamiya Nanako , Iso Hiroyasu 
Published: February 2023

AbstractAims/IntroductionWe aimed to examine the proportion of subsequent clinic visits for persons screened as having hyperglycemia based on glycated hemoglobin (HbA1c) levels at screening and the presence/absence of hyperglycemia at health checkups before 1 year of the screening among those without previous diabetes‐related medical care and attending regular clinic visits.Materials and methodsThis retrospective cohort study used the 2016–2020 data of Japanese health checkups and claims. The study analyzed 8,834 adult beneficiaries aged 20–59 years without regular clinic visits who had never received diabetes‐related medical care and whose recent health checkups showed hyperglycemia. The rates of 6‐month subsequent clinic visits after health checkups were evaluated according to HbA1c levels and the presence/absence of hyperglycemia at checkups a year before.ResultsThe overall clinic visit rate was 21.0%. The HbA1c‐specific rates were 17.0, 26.7, 25.4 and 28.4% for <7.0, 7.0–7.4, 7.5–7.9 and ≥8.0% (64 mmol/mol), respectively. Persons with hyperglycemia at a previous screening had lower clinic visit rates than those without hyperglycemia, particularly in the HbA1c category of <7.0% (14.4% vs 18.5%; P < 0.001) and 7.0–7.4% (23.6% vs 35.1%; P < 0.001).ConclusionsThe overall rate of subsequent clinic visits among those without previous regular clinic visits was <30%, including for participants with HbA1c ≥8.0%. Persons with previously detected hyperglycemia had lower clinic visit rates, despite requiring more health counseling. Our findings might be useful for designing a tailored approach to encourage high‐risk individuals to seek diabetes care through clinic visits.
Crafting Personal territory in a political world: A human agency approach to organizational politics
Authors: Liang Jian , Chen Hui , Du Danyang , He Xiao (CiCi) 
Published: February 2023

AbstractTo examine human agency in organizational politics, this study investigates how employees take action to craft their working environments in response to their perception of organizational politics (POP). In Study 1, we manipulated POP using video materials in an experiment. Results from 209 participants show that POP triggered the intention to engage in both relational crafting and task crafting behaviours. In Study 2, we collected survey data from 176 supervisor–subordinate dyads. Our results replicate the findings of Study 1. Furthermore, we found significant indirect relationships between POP and two outcome variables (i.e., task performance and opportunistic behaviour) through relational crafting. The two indirect relationships were stronger for employees with lower emotional intelligence. Meanwhile, significant indirect relationships existed between POP and two outcome variables (i.e., opportunistic behaviour and turnover intention) through task crafting and these indirect relationships were stronger for employees with higher emotional intelligence. Both theoretical and managerial implications are discussed.
Duration of bite force reduction following a single injection of botulinum toxin in the masseter muscle bilaterally: A one‐year non‐randomized trial
Authors: Ågren Martin , Nanchaipruek Yanisorn , Phumariyapong Phumithep , Apinuntham Chalermkwan , Rakchart Sarawalai , Pettersson Mattias , Wanitphakdeedecha Rungsima 
Published: February 2023

AbstractBackgroundPrevious studies concerning the effect of botulinum toxin in masseter muscle have mainly reported effects observed through inspection of facial features or differences in pain levels. One systematic review of studies utilizing objective measurements reported that long‐term muscular effect of botulinum neurotoxin injections into masseter muscle was inconclusive.ObjectiveTo evaluate the duration of reduced maximal voluntary bite force (MVBF) after botulinum toxin intervention.MethodsThe intervention group was comprised of individuals seeking aesthetic treatment for masseter reduction (n = 20), the reference group (n = 12) comprised of individuals with no intervention. Intervention through 25 units of Xeomin® (Merz Pharma GmbH & Co KGaA, Frankfurt am Main, Germany) botulinum neurotoxin type A injected into the masseter muscles bilaterally (totalling 50 units). A reference group did not receive any intervention. MVBF was measured in Newtons using a strain gauge meter at the incisors and first molars. MVBF was measured at baseline, at 4 weeks, 3 months, 6 months, and after 1 year.ResultsBoth groups were similar in terms of bite force, sex and age at baseline. MVBF remained similar compared to baseline in the reference group. At 3 months, a significant reduction at all measurement points was observed in the intervention group; at 6 months, this reduction was no longer significant.ConclusionA single intervention of 50 units of botulinum neurotoxin results in a reversible MVBF reduction of at least 3 months, although a visually discernable reduction may be more long‐lasting.
Laser ablation tomography (LATscan) as a new tool for anatomical studies of woody plants
Authors: Cunha Neto Israel L. , Hall Benjamin T. , Lanba Asheesh R. , Blosenski Joshua D. , Onyenedum Joyce G. 
Published: February 2023

Summary

Traditionally, botanists study plant anatomy by carefully sectioning samples, histological staining to highlight tissues of interests, then imaging slides under light microscopy. This approach generates significant details; however, this workflow is laborious, particularly in woody vines (lianas) with heterogeneous anatomies, and ultimately yields two‐dimensional (2D) images. Laser ablation tomography (LATscan) is a high‐throughput imaging system that yields hundreds of images per minute. This method has proven useful for studying the structure of delicate plant tissues; however, its utility in understanding the structure of woody tissues is underexplored.

We report LATscan‐derived anatomical data from several stems of lianas (c. 20 mm) of seven species and compare these results with those obtained through traditional anatomical techniques.

LATscan successfully allows the description of tissue composition by differentiating cell type, size, and shape, but also permits the recognition of distinct cell wall composition (e.g. lignin, suberin, cellulose) based on differential fluorescent signals on unstained samples.

LATscan generate high‐quality 2D images and 3D reconstructions of woody plant samples; therefore, this new technology is useful for both qualitative and quantitative analyses. This high‐throughput imaging technology has the potential to bolster phenotyping of vegetative and reproductive anatomy, wood anatomy, and other biological systems.
Metabolism of periodontal pathobionts: Their regulatory roles in the dysbiotic microbiota
Authors: Ding Jing , Zhao Chuanjiang , Gao Li 
Published: February 2023

AbstractThe onset and development of periodontitis centers around microbiota dysbiosis and disrupted host responses. Dynamic metabolic activities of the subgingival microbiota modify the polymicrobial community, shape the microenvironment, and modulate the host response. A complicated metabolic network exists in interspecies interactions between periodontal pathobionts and commensals, which can lead to the development of dysbiotic plaque. Dysbiotic subgingival microbiota undergo metabolic interactions with the host and disrupt host–microbe equilibrium. In this review, we discuss the metabolic profiles of the subgingival microbiota, the metabolic crosstalk in polymicrobial communities, including pathobionts and commensals, and the metabolic interactions between microbes and the host.
EvaluatingEEGcomplexity metrics as biomarkers for depression
Authors: Lord Brian , Allen John J. B. 
Published: February 2023

AbstractNonlinear EEG analysis offers the potential for both increased diagnostic accuracy and deeper mechanistic understanding of psychopathology. EEG complexity measures have previously been shown to positively correlate with clinical depression. In this study, resting state EEG recordings were taken across multiple sessions and days with both eyes open and eyes closed conditions from a total of 306 subjects, 62 of which were in a current depressive episode, and 81 of which had a history of diagnosed depression but were not currently depressed. Three different EEG montages (mastoids, average, and Laplacian) were also computed. Higuchi fractal dimension (HFD) and sample entropy (SampEn) were calculated for each unique condition. The complexity metrics showed high internal consistency within session and high stability across days. Higher complexity was found in open‐eye recordings compared to closed eyes. The predicted correlation between complexity and depression was not found. However, an unexpected sex effect was observed, in which males and females exhibited different topographic patterns of complexity.
Who contributes to disaster preparedness? Predicting decision making in social dilemmas pertaining to community resilience
Authors: Johnson Paul M. , Baroud Hiba , Brady Corey E. , Abkowitz Mark 
Published: February 2023

AbstractPlanning for community resilience through public infrastructure projects often engenders problems associated with social dilemmas, but little work has been done to understand how individuals respond when presented with opportunities to invest in such developments. Using statistical learning techniques trained on the results of a web‐based common pool resource game, we analyze participants' decisions to invest in hypothetical public infrastructure projects that bolster their community's resilience to disasters. Given participants' dispositions and in‐game circumstances, Bayesian additive regression tree (BART) models are able to accurately predict deviations from players' decisions that would reasonably lead to Pareto‐efficient outcomes for their communities. Participants tend to overcontribute relative to these Pareto‐efficient strategies, indicating general risk aversion that is analogous to individuals purchasing disaster insurance even though it exceeds expected actuarial costs. However, higher trait Openness scores reflect an individual's tendency to follow a risk‐neutral strategy, and fewer available resources predict lower perceived utilities derived from the infrastructure developments. In addition, several input variables have nonlinear effects on decisions, suggesting that it may be warranted to use more sophisticated statistical learning methods to reexamine results from previous studies that assume linear relationships between individuals' dispositions and responses in applications of game theory or decision theory.
Beyond path dependency: Analysing Indonesia's social policy responses to two crises
Authors: Yuda Tauchid Komara 
Published: February 2023

AbstractThis article sets out a coherent framework for debates and discussions on social policy changes during crisis periods. Using Indonesia as a case, this article compares its social policy responses to the 1997–1998 economic crisis and the current COVID‐19 pandemic crisis. Both two crises and their responses, once analysed, lead to an argument that path dependence does not always dominate policy processes, as most literature claims, but rather it operates alongside other path‐creation‐related processes, such as path‐clearing policy accelerators and path‐creation policy accelerators. This article also presents that ‘old player’ penetration in policy processes does not necessarily preclude alternatives and, in some examined cases, it, instead, strengthens impulses toward substantial reforms. Therefore, revised policies must be viewed as political vehicles for seeking popular support and power reconsolidation as democracy allows. Furthermore, this current analysis contributes to enhancing our understanding of crises, often seen as issues in public policy literature, yet less explored in terms of their importance as unique critical junctures and their implications for social policy changes in multiple paths.
Disruption of day‐to‐night changes in circadian gene expression with chronic tendinopathy
Authors: Yeung Ching‐Yan Chloé , Svensson René B. , Yurchenko Kateryna , Malmgaard‐Clausen Nikolaj M. , Tryggedsson Ida , Lendal Marius , Jokipii‐Utzon Anja , Olesen Jens L. , Lu Yinhui , Kadler Karl E. , Schjerling Peter , Kjær Michael 
Published: February 2023

AbstractOveruse injury in tendon tissue (tendinopathy) is a frequent and costly musculoskeletal disorder and represents a major clinical problem with unsolved pathogenesis. Studies in mice have demonstrated that circadian clock‐controlled genes are vital for protein homeostasis and important in the development of tendinopathy. We performed RNA sequencing, collagen content and ultrastructural analyses on human tendon biopsies obtained 12 h apart in healthy individuals to establish whether human tendon is a peripheral clock tissue and we performed RNA sequencing on patients with chronic tendinopathy to examine the expression of circadian clock genes in tendinopathic tissues. We found time‐dependent expression of 280 RNAs including 11 conserved circadian clock genes in healthy tendons and markedly fewer (23) differential RNAs with chronic tendinopathy. Further, the expression of COL1A1 and COL1A2 was reduced at night but was not circadian rhythmic in synchronised human tenocyte cultures. In conclusion, day‐to‐night changes in gene expression in healthy human patellar tendons indicate a conserved circadian clock as well as the existence of a night reduction in collagen I expression.
imageKey points
Tendinopathy is a major clinical problem with unsolved pathogenesis.
Previous work in mice has shown that a robust circadian rhythm is required for collagen homeostasis in tendons.
The use of circadian medicine in the diagnosis and treatment of tendinopathy has been stifled by the lack of studies on human tissue.
Here, we establish that the expression of circadian clock genes in human tendons is time dependent, and now we have data to corroborate that circadian output is reduced in diseased tendon tissues.
We consider our findings to be of significance in advancing the use of the tendon circadian clock as a therapeutic target or preclinical biomarker for tendinopathy.
Acute Mechanical and Metabolic Responses to Different Resistance Training Protocols With Equated Volume Load
Authors: Sánchez-Moreno Miguel , Rodiles-Guerrero Luis , Rendeiro-Pinho Gonçalo , Prieto-Veloso António , Pareja-Blanco Fernando 
Published: February 2023

Purpose: To investigate the effect of different resistance training protocols with equated volume load on acute mechanical and metabolic responses. Methods: In a randomized order, 18 men performed 8 different training protocols in the bench press exercise consisting of (sets, repetitions, intensity, and interset recoveries) 3 × 16, 40% 1-repetition maximum (1RM), 2 and 5 minutes; 6 × 8, 40% 1RM, 2 and 5 minutes; 3 × 8, 80% 1RM, 2 and 5 minutes; and 6 × 4, 80% 1RM, 2 and 5 minutes. Volume load was equalized between protocols (1920 arbitrary units). Velocity loss and effort index were calculated during the session. Movement velocity against the 60% 1RM and blood lactate concentration pre–post exercise were used to assess the mechanical and metabolic responses, respectively. Results: Resistance training protocols performed with heavy load (80% 1RM) resulted in a lower (P < .05) total number of repetitions (effect size = −2.44) and volume load (effect size = −1.79) than the scheduled ones when longer set configurations and shorter rest periods were used in the same protocol (ie, higher-training-density protocols). Protocols including a higher number of repetitions per set and shorter rest times induced higher velocity loss, effort index, and lactate concentrations than the rest of the protocols. Conclusions: Our results suggest that resistance training protocols with similar volume load but different training variables (ie, intensity, number of sets and repetitions, rest between sets) produce different responses. Implementing a lower number of repetitions per set and longer rest intervals is recommended to reduce the intrasession and postsession fatigue.
Kilohertz Frequency Alternating Current Induces Less Evoked Torque and Less Neuromuscular Efficiency Than Pulsed Current in Healthy People: A Randomized Crossover Trial
Authors: Paz Isabel de Almeida , Sonda Francesca Chaida , Fröhlich Matias , Durigan João Luiz Quagliotti , Vaz Marco Aurélio 
Published: February 2023

Context: Pulsed current and kilohertz frequency alternating current are 2 types of neuromuscular electrical stimulation (NMES) currents often used by clinicians during rehabilitation. However, the low methodological quality and the different NMES parameters and protocols used in several studies might explain their inconclusive results in terms of their effects in the evoked torque and the discomfort level. In addition, the neuromuscular efficiency (ie, the NMES current type that evokes the highest torque with the lowest current intensity) has not been established yet. Therefore, our objective was to compare the evoked torque, current intensity, neuromuscular efficiency (evoked torque/current intensity ratio), and discomfort between pulsed current and kilohertz frequency alternating current in healthy people. Design: A double-blind, randomized crossover trial. Methods: Thirty healthy men (23.2 [4.5] y) participated in the study. Each participant was randomized to 4 current settings: 2 kilohertz frequency alternating currents with 2.5 kHz of carrier frequency and similar pulse duration (0.4 ms) and burst frequency (100 Hz) but with different burst duty cycles (20% and 50%) and burst durations (2 and 5 ms); and 2 pulsed currents with similar pulse frequency (100 Hz) and different pulse duration (2 and 0.4 ms). The evoked torque, current intensity at the maximal tolerated intensity, neuromuscular efficiency, and discomfort level were evaluated. Results: Both pulsed currents generated higher evoked torque than the kilohertz frequency alternating currents, despite the similar between-currents discomfort levels. The 2 ms pulsed current showed lower current intensity and higher neuromuscular efficiency compared with both alternated currents and with the 0.4 ms pulsed current. Conclusions: The higher evoked torque, higher neuromuscular efficiency, and similar discomfort of the 2 ms pulsed current compared with 2.5-kHz frequency alternating current suggests this current as the best choice for clinicians to use in NMES-based protocols.
Prescribe, Review, Now!: an assessment of adequate PRN analgesia and associated laxative prescribing using Hospital Electronic Prescribing and Medicines Administration (HEPMA)
Authors: McMillan Matthew , Burgess Alexandra Jayne 
Published: February 2023

IntroductionOn HEPMA there is no way to notify a prescriber if patients are regularly accessing PRN (as-required) analgesia. We aimed to assess how well PRN analgesia use is identified, the WHO analgesic ladder and whether laxatives were prescribed with opioid analgesia.Methods3 data collection cycles were carried out for all medical inpatients between February-April 2022. Medication was reviewed to determine: 1) PRN analgesia prescribed? 2) Is the patient accessing it >3 times in 24hours? 3) Con-current laxatives prescribed. Between each cycle, an intervention was implemented. Intervention 1: Posters were placed on each ward and circulated electronically as a cue to a review and change analgesia “Prescribe. Review. Now!” Intervention 2: A presentation on data, the WHO analgesic ladder and laxative prescribing was created, and circulated.ResultsSee Figure 1 – Comparison of prescribing per cycle. Cycle 1 - 167 inpatients surveyed, 58%female, 42%male, mean age 78(±13.4). Cycle 2 - 159 inpatients,65% female, 35% male, mean age of 77 (±15.7). Cycle 3 - 157 inpatients, 62% female, 38% male, mean age 78 (±15.7). Adequate prescriptions on HEPMA improved by a total of 31% (p<0.005), over 3 cycles and 2 interventions.ConclusionsAfter each intervention there was a significant statistical improvement in prescribing analgesia and laxatives. However, there is still room for further improvement, especially in ensuring adequate laxative cover is prescribed for all patients either >65 years old, or those on opioid-based analgesia. Visual reminders on wards of regularly checking PRN medication showed to be an effective intervention.
Seven cases of hereditary haemorrhagic telangiectasia-like hepatic vascular abnormalities associated withEPHB4pathogenic variants
Authors: Guilhem Alexandre , Dupuis-Girod Sophie , Espitia Olivier , Rivière Sophie , Seguier Julie , Kerjouan Mallorie , Lavigne Christian , Maillard Hélène , Magro Pascal , Alric Laurent , Lipsker Dan , Parrot Antoine , Leguy Vanessa , Vanlemmens Claire , Guibaud Laurent , Vikkula Miikka , Eyries Melanie , Valette Pierre-Jean , Giraud Sophie 
Published: February 2023

BackgroundEPHB4loss of function is associated with type 2 capillary malformation–arteriovenous malformation syndrome, an autosomal dominant vascular disorder. The phenotype partially overlaps with hereditary haemorrhagic telangiectasia (HHT) due to epistaxis, telangiectases and cerebral arteriovenous malformations, but a similar liver involvement has never been described.MethodsMembers of the French HHT network reported their cases ofEPHB4mutation identified after an initial suspicion of HHT. Clinical, radiological and genetic characteristics were analysed.ResultsAmong 21 patients withEPHB4, 15 had a liver imaging, including 7 with HHT-like abnormalities (2 female patients and 5 male patients, ages 43–69 years). Atypical epistaxis and telangiectases were noted in two cases each. They were significantly older than the eight patients with normal imaging (median: 51 vs 20 years, p<0.0006).The main hepatic artery was dilated in all the cases (diameter: 8–11 mm). Six patients had hepatic telangiectases. All kind of shunts were described (arteriosystemic: five patients, arterioportal: two patients, portosystemic: three patients). The overall liver appearance was considered as typical of HHT in six cases.SixEPHB4variants were classified as pathogenic and one as likely pathogenic, with no specific hot spot.ConclusionEPHB4loss-of-function variants can be associated with HHT-like hepatic abnormalities and should be tested for atypical HHT presentations.