bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Combined DNA/RNA-based Next-Generation Sequencing Platform to Improve the Classification of Pancreatic Cysts and Early Detection of Pancreatic Cancer Arising From Pancreatic Cysts
Authors: Nikiforova Marina N. , Wald Abigail I. , Spagnolo Daniel M. , Melan Melissa A. , Grupillo Maria , Lai Yi-Tak , Brand Randall E. , O’Broin-Lennon Anne Marie , McGrath Kevin , Park Walter G. , Pfau Patrick R. , Polanco Patricio M. , Kubiliun Nisa , DeWitt John , Easler Jeffrey J. , Dam Aamir , Mok Shaffer R. , Wallace Michael B. , Kumbhari Vivek , Boone Brian A. , Marsh Wallis , Thakkar Shyam , Fairley Kimberly J. , Afghani Elham , Bhat Yasser , Ramrakhiani Sanjay , Nasr John , Skef Wasseem , Thiruvengadam Nikhil R. , Khalid Asif , Fasanella Kenneth , Chennat Jennifer , Das Rohit , Singh Harkirat , Sarkaria Savreet , Slivka Adam , Gabbert Charles , Sawas Tarek , Tielleman Thomas , Vanderveldt Hendrikus Dutch , Tavakkoli Anna , Smith Lynette M. , Smith Katelyn , Bell Phoenix D. , Hruban Ralph H. , Paniccia Alessandro , Zureikat Amer , Lee Kenneth K. , Ongchin Melanie , Zeh Herbert , Minter Rebecca , He Jin , Nikiforov Yuri E. , Singhi Aatur D. 
Published: May 2023

Objective:
We report the development and validation of a combined DNA/RNA next-generation sequencing (NGS) platform to improve the evaluation of pancreatic cysts.

Background and Aims:
Despite a multidisciplinary approach, pancreatic cyst classification, such as a cystic precursor neoplasm, and the detection of high-grade dysplasia and early adenocarcinoma (advanced neoplasia) can be challenging. NGS of preoperative pancreatic cyst fluid improves the clinical evaluation of pancreatic cysts, but the recent identification of novel genomic alterations necessitates the creation of a comprehensive panel and the development of a genomic classifier to integrate the complex molecular results.

Methods:
An updated and unique 74-gene DNA/RNA-targeted NGS panel (PancreaSeq Genomic Classifier) was created to evaluate 5 classes of genomic alterations to include gene mutations (e.g., KRAS, GNAS, etc.), gene fusions and gene expression. Further, CEA mRNA (CEACAM5) was integrated into the assay using RT-qPCR. Separate multi-institutional cohorts for training (n=108) and validation (n=77) were tested, and diagnostic performance was compared to clinical, imaging, cytopathologic, and guideline data.

Results:
Upon creation of a genomic classifier system, PancreaSeq GC yielded a 95% sensitivity and 100% specificity for a cystic precursor neoplasm, and the sensitivity and specificity for advanced neoplasia were 82% and 100%, respectively. Associated symptoms, cyst size, duct dilatation, a mural nodule, increasing cyst size, and malignant cytopathology had lower sensitivities (41–59%) and lower specificities (56–96%) for advanced neoplasia. This test also increased the sensitivity of current pancreatic cyst guidelines (IAP/Fukuoka and AGA) by >10% and maintained their inherent specificity.

Conclusions:
PancreaSeq GC was not only accurate in predicting pancreatic cyst type and advanced neoplasia but also improved the sensitivity of current pancreatic cyst guidelines.
Fecal Microbiota Transplant in Pediatric Solid Organ Transplant Recipients
Authors: Rodig Nancy M. , Weatherly Madison , Kaplan Abby L. , Ballal Sonia Arora , Elisofon Scott A. , Daly Kevin P. , Kahn Stacy A. 
Published: May 2023

Background.
Fecal microbiota transplant (FMT) is an effective treatment for recurrent Clostridioides difficile infection (CDI). Safety concerns around FMT are increased in immunocompromised populations, such as solid organ transplant (SOT) recipients. Outcomes among adult SOT recipients suggest FMT is efficacious and safe; however, pediatric SOT data are lacking.

Methods.
We describe the efficacy and safety of FMT among pediatric SOT recipients in a single-center retrospective study from March 2016 to December 2019. Successful FMT was defined as no recurrence of CDI within 2 mo of FMT. We identified 6 SOT recipients ages 4–18 y who received FMT a median of 5.3 y post-SOT.

Results.
Success after a single FMT was 83.3%. One liver recipient did not achieve cure after 3 FMTs and remains on low-dose vancomycin. One serious adverse event (SAE) occurred; cecal perforation and bacterial peritonitis occurred following colonoscopic FMT coordinated with intestinal biopsy in a kidney transplant recipient. He achieved full recovery and CDI cure. There were no other SAEs. There were no adverse events related to immunosuppression or transplantation status including: bacteremia, cytomegalovirus activation or reactivation, allograft rejection, or allograft loss.

Conclusions.
In this limited series, efficacy of FMT in pediatric SOT is comparable to efficacy in the general pediatric recurrent CDI population. There may be an increased risk of procedure-related SAE in SOT patients and larger cohort studies are needed.
Multiple organizational capabilities for effective business model innovation and increased performance
Authors: Kafetzopoulos Dimitrios , Samara Elpida Tryfon , Theodorakioglou Ypatia 
Published: May 2023

PurposeThe main purpose of this study is to provide a relatively integrated perspective on the role of market orientation, strategic flexibility and technological capability as multiple organizational capabilities and their potential effectiveness in promoting business model innovation (BMI) and business performance.Design/methodology/approachTo achieve this objective, an empirical survey was conducted among 379 firms in Greece. Initially, exploratory factor analysis and then confirmatory factor analysis were applied. Finally, the structural relationships among the latent factors were determined through structural equation modeling.FindingsThe results show the positive effect of market orientation on strategic flexibility and technological capability. Strategic flexibility drives firms to BMI and then business performance. Furthermore, it is essential that firms build up technological capability to be effective in BMI and business performance.Research limitations/implicationsData were collected at only one point in time from one country, Greece. This might pose limitations on the generalizability of our results. Future research could also explore how organizations develop strategic flexibility and BMI capabilities in different environmental contexts and organizational structures.Practical implicationsThis study sends the message that companies focusing on market orientation and technological capability can led to higher strategic flexibility and BMI capabilities, which in turn act as a catalyst for business improvement.Originality/valueThe proposed model provides plausible guidelines that advance the research on multiple organizational capabilities in companies.
Coerced work during parole: Prevalence, mechanisms, and characteristics
Authors: Augustine Dallas 
Published: May 2023

AbstractCoerced work on parole occurs when people are required to work under the threat of criminal legal repercussions. In the face of barriers to “good” work for people after prison, coercion helps to funnel parolees into positions at the bottom of the labor market. Parolee workers in these positions experience issues common to precarious, low‐wage work (low pay, hazardous working conditions, and labor law violations), as well as heightened vulnerability to predatory employers and exposure to parole‐prohibited activities. Because of the threat‐backed requirements to work, however, parolees must choose to either accept this “bad” work or face potential sanctions. Using mixed‐methods, including a novel form of respondent‐driven sampling I call “Hybrid‐RDS,” this article documents the prevalence of coerced work for people on parole in Los Angeles County, identifies the mechanisms through which coercive work operates, and illustrates the problematic employment conditions of coerced work after prison.
The rough‐toothed dolphin genome provides new insights into the genetic mechanism of its rough teeth
Authors: HUANG Xin , FAN Huizhong , ZHOU Wenliang , YANG Guang , WEI Fuwen 
Published: May 2023

AbstractThe rough‐toothed dolphin (Steno bredanensis) is characterized by having teeth covered in finely wrinkled vertical ridges, which is a general manifestation of amelogenesis imperfecta. The rough surfaces are hypothesized to be an evolutionary morphological trait of feeding adaptation to increase the dolphin's grip on prey. Here, we assembled a rough‐toothed dolphin genome and performed the comparative genomic analysis to reveal the genetic basis of the special enamel. Results showed that genes related to enamel development or dental diseases have undergone diversified adaptive changes that may shape the special enamel morphology of this dolphin species, including positive selection (CLDN19, PRKCE, SSUH2, and WDR72), rapid evolution (LAMB3), or unique amino acid substitutions (AMTN, ENAM, MMP20, and KLK4). Meanwhile, the historical demography of rough‐toothed dolphin indicated several distinct population fluctuations associated with climate change. The genome‐wide heterozygosity of this dolphin is in the middle of all published data for cetaceans. Although the population is considerable, there may be population or subspecies differentiation, and with the global warming and the increasing disturbance of human activities, we should pay more attention to protection in the future. Together, our study brings new insights into the genetic mechanisms that may have driven the evolution of the special enamel morphology in rough‐toothed dolphins and provides the first results of genetic heterozygosity and population historical dynamics of this species, which have important guiding implications for the conservation of this dolphin species.
Individual placement and support programmes for young adults: Where are they and whom do they serve?
Authors: Al‐Abdulmunem Monirah , Bond Gary R. , Ressler Daniel R. , Becker Deborah R. , Swanson Sarah J. , Marbacher Jessica 
Published: May 2023

AbstractAimIndividual Placement and Support (IPS), an evidence‐based supported employment model developed for adults with serious mental illness, has been recently targeted to young adults with mental health conditions, but little is known about its adoption in this age group in the United States.MethodsWe recruited a volunteer sample of nine IPS programmes in five states serving young adults with mental health conditions aged 16 to 24. IPS team leaders reported programme and participant characteristics and rated barriers to employment and education.ResultsMost IPS programmes were located in community mental health centres, served a small number of young adults, and received most referrals from external sources. The study sample of 111 participants included 53% female, 47% under 21 years old, 60% diagnosed with a depressive disorder; 92% had an employment goal, and 40% had an education goal. IPS specialists reported that managing mental health symptoms was the most common barrier to achieving employment and education goals.ConclusionFuture research should examine how IPS programmes could best provide services to young adults.
HUGO BALL'S RELIGIOUS CONVERSION
Authors: Lewer Deborah 
Published: May 2023

ABSTRACTThis essay investigates the German ex‐Dadaist Hugo Ball (1886–1927) and his 1920s work on religious conversion from Paul, Augustine and Francis to writers and poets in modernity. This intense engagement was rooted in Ball's own radical conversion, or ‘re‐conversion’, to an austere form of the Catholicism of his childhood in 1920, just a few years after breaking with the Dada movement he had helped found in Zurich in 1916. In letters, books, his edited diaries and essays such as ‘Die religiöse Konversion’ of 1925, Ball wrestled with the phenomenon of conversion. He traced it in religious culture, monasticism, psychiatry and politics. This article explores Ball's imaginative emphasis on the condition of chaos that precedes resolution into ‘order’ in the convert. It considers his model of conversion not only as salvific but also as remedial and therapeutic. Further, it interrogates his connection of conversion with the breakdown of language in mysticism and in Dada. Ball's intensive study of the mystical theology of Pseudo‐Dionysius the Areopagite and others provided much of the epistemological ground for this work. More provocative was his imagined possibility of collective national ‘conversion’ to Catholicism – for the whole of Germany.
Thrombocytosis and eosinophilia in 32 Chinese neonatal incontinentia pigmenti
Authors: Su Yufei , Zhang Huifang , Zou Zongyi , Ma Yingge , Zhang Huiping , Wen Jun , Li Hui 
Published: May 2023

AbstractIntroductionIncontinentia pigmenti (IP) is a rare X‐linked dominant genetic disease affecting ectodermal tissue and often misdiagnosed in the neonatal period. The aim of this study was to highlight sequential clinical features and evaluate prognosis of the 32 neonatal IP patients.Material and methodsA retrospective descriptive analysis was performed, using the clinical, blood analytical, pathological, radiological, genetic, and followed‐up data of neonatal patients diagnosed with IP from 2010 to 2021, in Xi'an, China.ResultsOf the 32 patients, two (6.25%) were male. Thirty babies (93.75%) had eosinophilia (eosinophilic granulocyte count: 0.31–19.9 × 109, mean proportion of white blood cells: 20.98 ± 15.21%). Twenty babies (62.5%) had thrombocytosis (thrombocyte count: 139–975 × 109, mean count: 416.76 ± 176.82). Thirty‐one babies (96.88%) exhibited the first three cutaneous lesion stages characterized by erythema and superficial vesicles on inflammatory bases in a linear distribution in the first week of age. Thirteen babies (40%) combined nervous system abnormalities, and nine babies (28.13%) had retinopathy. Two types of genetic mutations were detected in the NEMO gene. Nineteen babies were followed up. According to the follow‐up, four babies displayed psychomotor retardation, and five babies developed a decrease in vision with astigmatism and amblyopia.ConclusionIt is important that 30 babies (93.75%) had eosinophilia and 20 babies (62.5%) had thrombocytosis. Therefore, we speculate that the mechanism of the injury may be related to the platelet aggregation on the basis of the increase in eosinophil cells and the release of inflammatory factors.
Influence of feed moisture content on physical and sensory properties of extruded pure mung bean starch vermicelli
Authors: Nguyen Tien Phung , Lin Jenshinn , Songsermpong Sirichai 
Published: May 2023

SummaryThe extrusion process was studied to replace the traditional process of making mung bean vermicelli. The physical and sensory properties were studied of pure mung bean vermicelli made using a single‐screw extruder with different feed moisture contents of mung bean starch (35%, 40%, 45% or 50%). The degree of starch gelatinisation, water absorption, cooking loss and diameter of extruded vermicelli increased with increased feed moisture content. The extrusion temperature provided a more transparent vermicelli product at a higher feed moisture content. Compared with the commercial product using the traditional method, the extruded mung bean vermicelli had greater lightness (L*) and yellowness (b*) for the dried vermicelli and greater blueness (b*) for the cooked vermicelli. The tensile strength and elasticity decreased significantly with increased feed moisture content. The extruded mung bean vermicelli was ranked as ‘well satisfied’ by the panellists, especially, the vermicelli with a feed moisture content of 50%, which had the highest score in overall acceptability compared with the other conditions, including the commercial vermicelli. In conclusion, the extrusion method could be applied to produce good quality, pure mung bean vermicelli instead of the traditional method.
Integration of olfactory and auditory cues eliciting parental behavior
Authors: McRae Briana R. , Andreu Valentine , Marlin Bianca Jones 
Published: May 2023

AbstractParental care is crucial for the survival of all mammalian species. Given the evolutionary importance of parenting, this behavioral repertoire must be supported by circuitry that is innate but also capable of learning and flexibility – adjusting to changing environmental demands. In rodents, parental care is triggered by the perception of cues emitted by a pup. Caregiver‐pup interactions are often composed of multimodal sensory stimuli that require caregivers to integrate across sensory modalities. In this review, we focus on two sensory modalities essential for the parental experience: smell and hearing. We examine how smell and hearing are combined with other senses to identify offspring in need of care. Understanding how multimodal stimuli are integrated in the caregiver brain to inform parental behavior is an important step in understanding the circuitry that underlies this complex and crucial behavioral repertoire. In this review, we will discuss recent advances in the field of rodent parental behavior, highlighting studies that have begun to disentangle the neural circuitry that processes the multisensory cues that are involved in caregiver‐offspring interactions.
CO2‐induced chloroplast movement in one cell‐layer moss leaves
Authors: Sugiyama Taichi , Terashima Ichiro 
Published: May 2023

AbstractCO2‐induced chloroplast movement was reported in the monograph by Gustav Senn in 1908: unilateral CO2 supply to the one cell‐layered moss leaves induced the positively CO2‐tactic periclinal arrangement of chloroplasts. Here, using the model moss plant Physcomitrium patens, we examined basic features of chloroplast CO2‐tactic relocation with a modernized experimental system. The CO2 relocation was light‐dependent and, especially, CO2 relocation in red light was substantially dependent on photosynthetic activity. In blue light, CO2 relocation was mainly dependent on microfilaments while microtubule‐based movement was insensitive to CO2, whereas in red light, both cytoskeletons contributed redundantly to CO2 relocation. The CO2 relocation was observed not only when the two leaf surfaces were exposed to CO2‐free air versus CO2‐containing air, but also by exposing them physiologically relevant differences in CO2 concentrations. In the leaves placed on the surface of a gel sheet, chloroplasts avoided the gel side and positioned in the air‐facing surface, and this phenomenon was also shown to be photosynthesis dependent. Based on these observations, we propose a hypothesis that the threshold light intensity between the light‐accumulation and ‐avoidance responses of the photorelocation would be increased by CO2, resulting in the CO2‐tactic relocation of chloroplasts.
Multimodal study of the neural sources of error monitoring in adolescents and adults
Authors: Conte Stefania , Richards John E. , Fox Nathan A. , Valadez Emilio A. , McSweeney Marco , Tan Enda , Pine Daniel S. , Winkler Anderson M. , Liuzzi Lucrezia , Cardinale Elise M. , White Lauren K. , Buzzell George A. 
Published: May 2023

AbstractThe ability to monitor performance during a goal‐directed behavior differs among children and adults in ways that can be measured with several tasks and techniques. As well, recent work has shown that individual differences in error monitoring moderate temperamental risk for anxiety and that this moderation changes with age. We investigated age differences in neural responses linked to performance monitoring using a multimodal approach. The approach combined functional MRI and source localization of event‐related potentials (ERPs) in 12‐year‐old, 15‐year‐old, and adult participants. Neural generators of two components related to performance and error monitoring, the N2 and ERN, lay within specific areas of fMRI clusters. Whereas correlates of the N2 component appeared similar across age groups, age‐related differences manifested in the location of the generators of the ERN component. The dorsal anterior cingulate cortex (dACC) was the predominant source location for the 12‐year‐old group; this area manifested posteriorly for the 15‐year‐old and adult groups. A fMRI‐based ROI analysis confirmed this pattern of activity. These results suggest that changes in the underlying neural mechanisms are related to developmental changes in performance monitoring.
Investigation of the effects of syringic acid supplemented to Tris semen diluent on ram semen freezability
Authors: Avdatek Fatih , İnanç Muhammed Enes , Gülhan Mehmet Fuat , Güngör Şükrü , Yeni Deniz , Olğaç Kemal Tuna , Denk Barış , Taşdemir Umut 
Published: May 2023

AbstractTo the best of our knowledge, no research has been conducted to test the effects of syringic acid (SA) on ram semen freezing within the scope of natural antioxidants added to semen extenders. Therefore, this study had two main objectives. First, to test whether adding SA to ram semen freezing extender has a protective effect and contributes positively to sperm kinetic, plasma and acrosome integrity, mitochondrial membrane potential, lipid peroxidation, oxidant and antioxidant and DNA damage parameters after thawing. Second, it was to determine at what concentration the SA supplemented to the extender could be applied by in vitro studies by preserving the fertilization ability of frozen semen at the highest level. In the study, six individuals of Sönmez rams were used. The semen was collected from the rams using an artificial vagina and pooled. The pooled semen was divided into five different groups and extended with 0, 0.5, 1, 2 and 4 mM SA (control C, SA0.5, SA1, SA2 and SA4, respectively). After dilution, the semen samples were kept at 4°C for 3 h, then loaded into 0.25 mL straws and frozen in liquid nitrogen vapour. The SA1 and SA2 groups were higher plasma membrane and acrosome integrity (PMAI), high mitochondrial membrane potential (HMMP), plasma membrane integrity and motility compared to other groups (p < .05). It was observed that SA supplemented to the Tris extender significantly reduced DNA damage, and the lowest values were obtained especially in the SA1 and SA2 treatments (p < .05). Also, lowest MDA level was determined at the SA1 and this was statistically significant compared to SA4 and C (p < .05). In conclusion, it was revealed that SA added to Tris semen extender at 1 and 2 mM treatment doses increased progressive and total motility and preserved PMAI, plasma membrane integrity, HMMP and DNA integrity.