bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the Educational Value of Popular Culture in Web-Based Medical Education: Pre-Post Study on Teaching Jaundice Using “The Simpsons”
Authors: Dalavaye Nishaanth , Baskaran Ravanth , Mukhopadhyay Srinjay , Gamage Movin Peramuna , Ng Vincent , Sharif Hama , Rutherford Stephen 
Published: August 2023

Background
The potential of popular culture as a tool for knowledge delivery and enhancing engagement in education is promising but not extensively studied. Furthermore, concerns exist regarding learning fatigue due to increased reliance on videoconferencing platforms following the COVID-19 pandemic. To ensure effective web-based teaching sessions that maintain attention spans and enhance understanding, innovative solutions are necessary.

Objective
This study aims to evaluate the use of specific popular culture case studies to enhance student engagement in a web-based near-peer teaching session.

Methods
We delivered a web-based teaching session to undergraduate medical students in the United Kingdom. The session included clinical vignettes and single-best-answer questions using characters from “The Simpsons” television show as patient analogies for various causes of jaundice. A pre-post survey, employing a 7-point Likert scale, was distributed to gather data from participants.

Results
A total of 53 survey responses were collected. Participants reported significantly improved understanding of jaundice after the session compared to before the session (median 6, IQR 5-6 vs median 4, IQR 3-4.5; P<.001). The majority of participants agreed that the inclusion of “The Simpsons” characters enhanced their knowledge and made the teaching session more memorable and engaging (memorability: median 6, IQR 5-7; engagement: median 6, IQR 5-7).

Conclusions
When appropriately integrated, popular culture can effectively engage students and improve self-perceived knowledge retention. “The Simpsons” characters can be used pedagogically and professionally as patient analogies to deliver teaching on the topic of jaundice.
Exploring the Effect of the Dynamics of Behavioral Phenotypes on Health Outcomes in an mHealth Intervention for Childhood Obesity: Longitudinal Observational Study
Authors: Woo Sarah , Jung Sunho , Lim Hyunjung , Kim YoonMyung , Park Kyung Hee 
Published: August 2023

Background
Advancements in mobile health technologies and machine learning approaches have expanded the framework of behavioral phenotypes in obesity treatment to explore the dynamics of temporal changes.

Objective
This study aimed to investigate the dynamics of behavioral changes during obesity intervention and identify behavioral phenotypes associated with weight change using a hybrid machine learning approach.

Methods
In total, 88 children and adolescents (ages 8-16 years; 62/88, 71% male) with age- and sex-specific BMI ≥85th percentile participated in the study. Behavioral phenotypes were identified using a hybrid 2-stage procedure based on the temporal dynamics of adherence to the 5 behavioral goals during the intervention. Functional principal component analysis was used to determine behavioral phenotypes by extracting principal component factors from the functional data of each participant. Elastic net regression was used to investigate the association between behavioral phenotypes and weight change.

Results
Functional principal component analysis identified 2 distinctive behavioral phenotypes, which were named the high or low adherence level and late or early behavior change. The first phenotype explained 47% to 69% of each factor, whereas the second phenotype explained 11% to 17% of the total behavioral dynamics. High or low adherence level was associated with weight change for adherence to screen time (β=−.0766, 95% CI −.1245 to −.0312), fruit and vegetable intake (β=.1770, 95% CI .0642-.2561), exercise (β=−.0711, 95% CI −.0892 to −.0363), drinking water (β=−.0203, 95% CI −.0218 to −.0123), and sleep duration. Late or early behavioral changes were significantly associated with weight loss for changes in screen time (β=.0440, 95% CI .0186-.0550), fruit and vegetable intake (β=−.1177, 95% CI −.1441 to −.0680), and sleep duration (β=−.0991, 95% CI −.1254 to −.0597).

Conclusions
Overall level of adherence, or the high or low adherence level, and a gradual improvement or deterioration in health-related behaviors, or the late or early behavior change, were differently associated with weight loss for distinctive obesity-related lifestyle behaviors. A large proportion of health-related behaviors remained stable throughout the intervention, which indicates that health care professionals should closely monitor changes made during the early stages of the intervention.

Trial Registration
Clinical Research Information Science KCT0004137; https://tinyurl.com/ytxr83ay
Aristotle’s Syllogistic as a Form of Geometry
Authors: Triantafyllou Vangelis 
Published: August 2023

Abstract
This article is primarily concerned with Aristotle’s theory of the syllogistic, and the investigation of the hypothesis that logical symbolism and methodology were in these early stages of a geometrical nature; with the gradual algebraization that occurred historically being one of the main reasons that some of the earlier passages on logic may often appear enigmatic. The article begins with a brief introduction that underlines the importance of geometric thought in ancient Greek science, and continues with a short exposition of Aristotle’s views and methods in regard to logic. We then offer an interpretation of syllogisms, as well as of the main proof methods that are utilized by Aristotle, in terms of diagrams that can be seen as analogous to those of Euclidean geometry; where the various proofs proceed by appropriately manipulating an initially drawn diagram, in a way parallel to constructions that occur in Euclid’s Elements. In this way, logic is presented as following, to a large degree, methodological tenets established by the practice of geometry. Finally, we present a diagrammatic decision procedure that allows one to directly read off the validity of syllogisms from a corresponding diagram, in a way such that no further manipulation of it is necessitated.
Implementasi Algoritma Klasifikasi Naïvebayes Untuk Menentukan Kesulitan Soal Quiz Menggunakan Renpy
Authors: Rosy Fathur , Wardhana Sukma 
Published: August 2023

Banyak game edukasi berjenis trivia dikembangkan dengan tujuan menguji kemampuan berfikir pemain. Namun, seringkali pemain menghadapi kesulitan dalam menjawab pertanyaan yang diberikan, Salah satu faktornya disebabkan oleh pertanyaan yang tidak seimbang, terlalu sulit atau terlampau mudah. Sehingga rasa monoton dan ketidaksesuaian dengan kemampuan si pemain membuat pengalaman bermain menjadi kurang memuaskan. Peluang untuk menghasilkan pertanyaan yang sesuai dengan kemampuan pemain sebenarnya bisa diprediksi berdasarkan data dari pemain dengan suatu metode. Salah satu metode yang bisa digunakan untuk mengatasi masalah ini dengan menggunakan teknik dalam data mining yaitu klasifikasi naïve bayes. Algoritma naïve bayes digunakan untuk mengklasifikasikan tingkat kesulitan soal berdasarkan parameter yang dihasilkan oleh pemain yaitu kecepatan menjawab soal,jawaban benar atau salah dan berapakali pemain salah dalam menjawab soal yang didesain dalam bentuk healtbar. Sedangkan parameter untuk keputusan tingkat kesulitan soal adalah Mudah, Sedang, dan Sulit. Hasil pengujian naïve bayes dengan sepuluh orang pemain mampu memberikan tingkat kesulitan soal yang sesuai dengan akurasi tertinggi sebesar 92.85% dan akurasi terendah sebesar 35.29% Sedangkan rata-rata akurasi yang didapat 66.5%. secara keseluruhan rata-rata akurasi Naïve Bayes menunjukkan tingkat keberhasilan yang cukup baik dalam pengujian tersebut.
Contestable Confessions of the Untranslatable: a Worldmaking Project
Authors: Wowk Lucy 
Published: August 2023

<p>This research-creation project is an experimental translation of Claude Cahunâ€™s book <em>Aveux non avenus </em>(1930), culminating in an artistsâ€™ book of image and text documenting the process. This approach suggests an alternative to biographization of the life and work of Cahun. It seeks to unpack how experimental translation, rooted in affect theory, might offer supplemental modes of interpretation and understanding that complicate, rather than render palatable, the complexity of a personsâ€™ life/work. The emergent genre of female philosophical fiction is simultaneously engaged and interrogatedâ€”questions of categorization and transgression become central to the process. The arts-based methodology seeks to unpack and understand genre from the inside out. Ultimately, translation is framed as a metaphor for understanding, and worldmaking emerges as vital to processes of reading and writing.Â </p>
Genome instability-derived genes as a novel prognostic signature for lung adenocarcinoma
Authors: Zhang Xu , Lam Tak-Wah , Ting Hing-Fung 
Published: August 2023

Background: An increasing number of patients are being diagnosed with lung adenocarcinoma, but there remains limited progress in enhancing prognostic outcomes and improving survival rates for these patients. Genome instability is considered a contributing factor, as it enables other hallmarks of cancer to acquire functional capabilities, thus allowing cancer cells to survive, proliferate, and disseminate. Despite the importance of genome instability in cancer development, few studies have explored the prognostic signature associated with genome instability for lung adenocarcinoma.Methods: In the study, we randomly divided 397 lung adenocarcinoma patients from The Cancer Genome Atlas database into a training group (n = 199) and a testing group (n = 198). By calculating the cumulative counts of genomic alterations for each patient in the training group, we distinguished the top 25% and bottom 25% of patients. We then compared their gene expressions to identify genome instability-related genes. Next, we used univariate and multivariate Cox regression analyses to identify the prognostic signature. We also performed the Kaplan–Meier survival analysis and the log-rank test to evaluate the performance of the identified prognostic signature. The performance of the signature was further validated in the testing group, in The Cancer Genome Atlas dataset, and in external datasets. We also conducted a time-dependent receiver operating characteristic analysis to compare our signature with established prognostic signatures to demonstrate its potential clinical value.Results: We identified GULPsig, which includes IGF2BP1, IGF2BP3, SMC1B, CLDN6, and LY6K, as a prognostic signature for lung adenocarcinoma patients from 42 genome instability-related genes. Based on the risk score of the risk model with GULPsig, we successfully stratified the patients into high- and low-risk groups according to the results of the Kaplan–Meier survival analysis and the log-rank test. We further validated the performance of GULPsig as an independent prognostic signature and observed that it outperformed established prognostic signatures.Conclusion: We provided new insights to explore the clinical application of genome instability and identified GULPsig as a potential prognostic signature for lung adenocarcinoma patients.
Proteomics and disease network associations evaluation of environmentally relevant Bisphenol A concentrations in a human 3D neural stem cell model
Authors: Horánszky Alex , Shashikadze Bachuki , Elkhateib Radwa , Lombardo Salvo Danilo , Lamberto Federica , Zana Melinda , Menche Jörg , Fröhlich Thomas , Dinnyés András 
Published: August 2023

Bisphenol A (BPA) exposure is associated with a plethora of neurodevelopmental abnormalities and brain disorders. Previous studies have demonstrated BPA-induced perturbations to critical neural stem cell (NSC) characteristics, such as proliferation and differentiation, although the underlying molecular mechanisms remain under debate. The present study evaluated the effects of a repeated-dose exposure of environmentally relevant BPA concentrations during the in vitro 3D neural induction of human induced pluripotent stem cells (hiPSCs), emulating a chronic exposure scenario. Firstly, we demonstrated that our model is suitable for NSC differentiation during the early stages of embryonic brain development. Our morphological image analysis showed that BPA exposure at 0.01, 0.1 and 1 µM decreased the average spheroid size by day 21 (D21) of the neural induction, while no effect on cell viability was detected. No alteration to the rate of the neural induction was observed based on the expression of key neural lineage and neuroectodermal transcripts. Quantitative proteomics at D21 revealed several differentially abundant proteins across all BPA-treated groups with important functions in NSC proliferation and maintenance (e.g., FABP7, GPC4, GAP43, Wnt-8B, TPPP3). Additionally, a network analysis demonstrated alterations to the glycolytic pathway, potentially implicating BPA-induced changes to glycolytic signalling in NSC proliferation impairments, as well as the pathophysiology of brain disorders including intellectual disability, autism spectrum disorders, and amyotrophic lateral sclerosis (ALS). This study enhances the current understanding of BPA-related NSC aberrations based mostly on acute, often high dose exposures of rodent in vivo and in vitro models and human GWAS data in a novel human 3D cell-based model with real-life scenario relevant prolonged and low-level exposures, offering further mechanistic insights into the ramifications of BPA exposure on the developing human brain and consequently, later life neurological disorders.
Clinical characteristics of respiratory tract infection caused by Klebsiella pneumoniae in immunocompromised patients: a retrospective cohort study
Authors: Liu Yahui , Huang Lin , Cai Jing , Zhu Haixing , Li Junjie , Yu Youchao , Xu Yumin , Shi Guochao , Feng Yun 
Published: August 2023

PurposeWith advancements in medical technology and the growth of an aging society, the number of immunocompromised patients has increased progressively. Klebsiella pneumoniae (K. pneumoniae) is one of the most common opportunistic pathogens, causing a severe disease burden. We aimed to further clarify the differences in respiratory tract K. pneumoniae infections between immunocompromised and immunocompetent populations.MethodsWe retrospectively compared cases of respiratory tract K. pneumoniae infection in immunocompromised and immunocompetent patients admitted to Ruijin Hospital in Shanghai between January 2019 and August 2020 to clarify the differences between the two groups.ResultsWe enrolled 400 immunocompromised patients and 386 immunocompetent patients. Compared to the immunocompetent group, immunocompromised patients were more likely to develop bacteremia and shock and to require mechanical ventilation support during hospitalization. Immunocompromised patients also had a greater probability of polymicrobial infection and a higher rate of antibacterial resistance to carbapenem, which resulted in a higher intensive care unit admission rate, 30-day case fatality rate (CFR), and 6-month CFR. Multivariate analysis indicated that immunocompromised patients with respiratory diseases (odds ratio [OR], 2.189; 95% confidence interval [CI], 1.103-4.344; P = 0.025) and cardiovascular diseases (OR, 2.008; 95% CI, 1.055-3.822; P = 0.034), using mechanical ventilation (OR, 3.982; 95% CI, 2.053-7.722; P = 0.000), or infected with multidrug-resistant K. pneumoniae (OR, 3.870; 95%, 1.577-9.498; P = 0.003) were more likely to have a higher 30-day CFR.ConclusionThe disease burden of K. pneumoniae infection in immunocompromised patients is high. Immunocompromised patients who presented with respiratory diseases and cardiovascular diseases, used mechanical ventilation, or were infected with multidrug-resistant K. pneumoniae experienced a higher 30-day mortality rate.
Ibrutinib impairs IGF-1-dependent activation of intracellular Ca handling in isolated mouse ventricular myocytes
Authors: Tarnowski Daniel , Feder Anna-Lena , Trum Maximilian , Kreitmeier Klaus-Georg , Stengel Laura , Maier Lars S. , Sag Can Martin 
Published: August 2023

BackgroundThe Bruton tyrosine kinase (BTK) inhibitor Ibrutinib is associated with a higher incidence of cardiotoxic side effects including heart failure (HF).ObjectivesIbrutinib is capable of inhibiting PI3K/Akt signaling in neonatal rat ventricular cardiomyocytes when stimulated with insulin-like growth factor 1 (IGF-1). We therefore hypothesized that Ibrutinib might disrupt IGF-1-mediated activation of intracellular Ca handling in adult mouse cardiomyocytes by inhibiting PI3K/Akt signaling.MethodsIsolated ventricular myocytes (C57BL6/J) were exposed to IGF-1 at 10 nmol/L in the presence or absence of Ibrutinib (1 µmol/L) or Acalabrutinib (10 µmol/L; cell culture for 24 ± 2 h). Intracellular Ca handling was measured by epifluorescence (Fura-2 AM) and confocal microscopy (Fluo-4 AM). Ruptured-patch whole-cell voltage-clamp was used to measure ICa. Levels of key cardiac Ca handling proteins were investigated by immunoblots.ResultsIGF-1 significantly increased Ca transient amplitudes by ∼83% as compared to vehicle treated control cells. This was associated with unaffected diastolic Ca, enhanced SR Ca loading and increased ICa. Co-treatment with Ibrutinib attenuated both the IGF-1-mediated increase in SR Ca content and in ICa. IGF-1 treated cardiomyocytes had significantly increased levels of pS473Akt/Akt and SERCA2a expression as compared to cells concomitantly treated with IGF-1 and Ibrutinib. SR Ca release (as assessed by Ca spark frequency) was unaffected by either treatment. In order to test for potential off-target effects, second generation BTK inhibitor Acalabrutinib with greater BTK selectivity and lower cardiovascular toxicity was tested for IGF1-mediated activation of intracellular Ca handling. Acalabrutinib induced similar effects on Ca handling in IGF-1 treated cultured myocytes as Ibrutinib in regard to decreased Ca transient amplitude and slowed Ca transient decay, hence implying a functional class effect of BTK inhibitors in cardiac myocytes.ConclusionsInhibition of BTK by Ibrutinib impairs IGF-1-dependent activation of intracellular Ca handling in adult ventricular mouse myocytes in the face of disrupted Akt signaling and absent SERCA2a upregulation.
Concomitant valve surgery is associated with worse outcomes in surgical treatments of post-infarction ventricular aneurysm
Authors: Liu Yuqi , Cai Ziwen , Xu Li , Zheng Yidan , Chen Ming , Dong Nianguo , Chen Si 
Published: August 2023

ObjectiveTo evaluate the impact of concomitant valve surgery on the prognosis of patients who experienced coronary artery bypass graft (CABG) with/without ventricular reconstruction for the ventricular aneurysm.MethodsIn our department, 354 patients underwent CABG with/without ventricular reconstruction for a ventricular aneurysm from July 23rd, 2000 to December 23rd, 2022. A total of 77 patients received concomitant valve surgery, 37 of whom underwent replacement, and 40 of whom underwent repair. The baseline characteristics, prognostic, and follow-up information were statically analyzed. Univariate and multivariate Cox regression analyses were applied to identify the risk factors of long-term outcomes.ResultsCompared with patients who did not undergo valvular surgery, patients who experienced concomitant valve surgical treatments had a significantly lower survival rate (p = 0.00022) and a longer total mechanical ventilation time. Subgroup analysis indicated that the options of repair or replacement exhibited no statistically significant difference in postoperative mortality (p = 0.44) and prognosis. The multivariate Cox regression analysis suggested that the pre-operative cholesterol level (HR = 1.68), postoperative IABP (HR = 6.29), NYHA level (HR = 2.84), and pre-operative triglyceride level (HR = 1.09) were independent and significant predictors for overall all-cause mortality after surgery.ConclusionConcomitant valve surgery was considerably related to a higher risk of postoperative mortality in patients with post-infarction ventricle aneurysms who underwent surgical treatments. No significant difference in the prognosis outcomes was observed between the operating methods of repair or replacement valve surgery.
Metabolic and molecular effects of dietary extra virgin olive oil in blood and placenta of women with GDM
Authors: Gomez Ribot Dalmiro , Diaz Esteban , Fazio María Victoria , Gómez Hebe Lorena , Careaga Valeria , Maier Marta , Macchi Silvia Beatriz , Gresta Carlos Alberto , Capobianco Evangelina , Jawerbaum Alicia 
Published: August 2023

Gestational diabetes mellitus (GDM) increases the risks of maternal, placental, and neonatal complications. Previously, we found that a diet enriched in extra virgin olive oil (EVOO) prevents increased maternal triglyceridemia and placental proinflammatory markers in a cohort of GDM patients. The aim of this work was to evaluate maternal circulating markers of insulin resistance, placental collagen, glycogen and lipid levels, and placental levels of proteins, mRNAs, and a microRNA involved in the endocytic pathway in the same cohort of control women and women with GDM who received or did not receive a diet enriched in EVOO (36 g/day) from weeks 24 to 28 of pregnancy until term.ResultsAt term, the TG/HDL cholesterol ratio, fatty acid binding protein 4 circulating levels, and maternal BMI were increased in the GDM patients, alterations prevented by the maternal diet enriched in EVOO. Although there were no changes in placental lipid levels and lipid profile, GDM placentas were thicker than controls and showed increased glycogen and collagen content, alterations prevented by the EVOO enriched diet. GDM placentas showed increases in megalin levels, in the expression of several genes involved in the endocytic pathway, and in miR-199, which targets these genes, alterations prevented by the maternal diet enriched in EVOO.ConclusionsWe identified novel beneficial effects of an EVOO-enriched diet in GDM women, a diet capable of regulating maternal insulin resistance, the structure and metabolism of the placenta, and the placental endocytic pathway, suggesting effects that may be beneficial for fetal development.
A comparative study of human and zebrafish glucocorticoid receptor activities of natural and pharmaceutical steroids
Authors: Toso Anna , Boulahtouf Abdelhay , Escande Aurélie , Garoche Clémentine , Balaguer Patrick 
Published: August 2023

IntroductionThe action of environmental steroids on the human glucocorticoid receptor (hGR) has been pointed out with the risk to impair physiological immune and metabolic processes regulated by this nuclear receptor. However, there is still a lack of mechanistic information regarding their ability to interact with GR in aquatic species.MethodsTo investigate ligand activation differences between hGR and zebrafish GR (zfGR), we tested several natural and synthetic steroids using reporter cell lines expressing hGR or zfGR.Results and discussionAlmost all the glucocorticoids tested (dexamethasone, cortisol, bimedrazol, medrol, cortivazol and fluticasone) are agonists of the two receptors with similar potencies. The dissociated glucocorticoids, RU24782 and RU24858 are agonists of both zfGR and hGR but with a better potency for the latter. On the other hand, the synthetic glucocorticoid forbimenol and the mineralocorticoid aldosterone are agonist on hGR but antagonist on zfGR. The other steroids tested, androgens and progestins, are all antagonists of both GRs with equal or lower potency on zfGR than on hGR. Surprisingly, the lower efficacy and potency on zfGR of aldosterone, forbimenol and the dissociated glucocorticoids is not related to their affinity for the receptors which would suggest that it could be related to less efficacious recruitment of coactivators by zfGR compared to hGR.