bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lower Myelin Content Is Associated With Lower Gait Speed in Cognitively Unimpaired Adults
Authors: Faulkner Mary E , Laporte John P , Gong Zhaoyuan , Akhonda Mohammad A B S , Triebswetter Curtis , Kiely Matthew , Palchamy Elango , Spencer Richard G , Bouhrara Mustapha 
Published: March 2023

AbstractMounting evidence indicates that abnormal gait speed predicts the progression of neurodegenerative diseases, including Alzheimer’s disease. Understanding the relationship between white matter integrity, especially myelination, and motor function is crucial to the diagnosis and treatment of neurodegenerative diseases. We recruited 118 cognitively unimpaired adults across an extended age range of 22–94 years to examine associations between rapid or usual gait speeds and cerebral myelin content. Using our advanced multicomponent magnetic resonance relaxometry method, we measured myelin water fraction (MWF), a direct measure of myelin content, as well as longitudinal and transverse relaxation rates (R1 and R2), sensitive but nonspecific magnetic resonance imaging measures of myelin content. After adjusting for covariates and excluding 22 data sets due to cognitive impairments or artifacts, our results indicate that participants with higher rapid gait speed exhibited higher MWF, R1, and R2 values, that is, higher myelin content. These associations were statistically significant within several white matter brain regions, particularly the frontal and parietal lobes, splenium, anterior corona radiata, and superior fronto-occipital and longitudinal fasciculus. In contrast, we did not find any significant associations between usual gait speed and MWF, R1, or R2, which suggests that rapid gait speed may be a more sensitive marker of demyelination than usual gait speed. These findings advance our understanding on the implication of myelination in gait impairment among cognitively unimpaired adults, providing further evidence of the interconnection between white matter integrity and motor function.
“The Dynamic Nature of Being a Person”: An Ethnographic Study of People Living With Dementia in Their Communities
Authors: Birt Linda , Charlesworth Georgina , Moniz-Cook Esme , Leung Phuong , Higgs Paul , Orrell Martin , Poland Fiona 
Published: March 2023

Abstract

Background and Objectives
A dementia diagnosis can affect social interactions. This study aims to understand how people living with dementia act as social beings within everyday interactions in their local communities.

Research Design and Methods
Focused ethnography informed by Spradley’s approach to data collection and analysis. Observations in community spaces.

Results
Twenty-nine observations were undertaken in everyday social settings with 11 people with dementia who were part of a longitudinal interview study. Data consisted of 40 hr of observation, and researcher field notes. The overarching theme “the dynamic nature of being a person” encapsulates participants’ exhibited experiences in negotiating to attain and sustain an acknowledged place in their communities. Two subthemes characterized contexts and actions: (1) “Being me—not dementia”: Participants constructed narratives to assert their ontological presence in social settings. They and others used strategies to mediate cognitive changes evidencing dementia. (2) “Resisting or acquiescing to ‘being absent in place’”: Participants were often able to resist being absent to the gaze from others, but some social structures and behaviors led to a person being “in place,” yet not having their presence confirmed.

Discussion and Implications
People living with dementia can actively draw on personal attributes, familiar rituals, objects, and social roles to continue to present themselves as social beings. Identifying how postdiagnosis people may self-manage cognitive changes to retain their presence as a person can help health and social care practitioners and families collaborate with the person living with dementia enabling them to have a continued social presence.
Cell-free expression and characterization of multivalent rhamnose-binding lectins using bio-layer interferometry
Authors: Warfel Katherine F , Laigre Eugénie , Sobol Sarah E , Gillon Emilie , Varrot Annabelle , Renaudet Olivier , Dejeu Jerome , Jewett Michael C , Imberty Anne 
Published: March 2023

Abstract
Lectins are important biological tools for binding glycans, but recombinant protein expression poses challenges for some lectin classes, limiting the pace of discovery and characterization. To discover and engineer lectins with new functions, workflows amenable to rapid expression and subsequent characterization are needed. Here, we present bacterial cell-free expression as a means for efficient, small-scale expression of multivalent, disulfide bond-rich, rhamnose-binding lectins. Furthermore, we demonstrate that the cell-free expressed lectins can be directly coupled with bio-layer interferometry analysis, either in solution or immobilized on the sensor, to measure interaction with carbohydrate ligands without purification. This workflow enables the determination of lectin substrate specificity and estimation of binding affinity. Overall, we believe that this method will enable high-throughput expression, screening, and characterization of new and engineered multivalent lectins for applications in synthetic glycobiology.
Correlates of motivation to quit waterpipe tobacco smoking among US young adults: implications for cessation interventions
Authors: Alalwan Mahmood A , Keller-Hamilton Brittney , Long Lauren , Lipkus Isaac , Wagener Theodore L , Mays Darren 
Published: March 2023

Abstract
Waterpipe tobacco smoking is a public health concern that poses many of the same health risks as cigarette smoking, especially among young adults—a subpopulation characterized by the highest prevalence of waterpipe tobacco smoking. Nevertheless, it remains understudied relative to other forms of tobacco use. We examined sociodemographic, behavioral and cognitive factors associated with young adults’ motivation to quit waterpipe smoking using a theory-informed approach. We completed a secondary analysis of baseline data on waterpipe tobacco smoking beliefs and behavior collected from 349 US young adults aged 18–30 years. We analyzed sociodemographics, tobacco use and cessation behaviors and perceptions, and theory-related constructs associated with motivation to quit waterpipe tobacco smoking using linear regression. Overall, participants reported low motivation (mean = 2.68, SD = 1.56, scale range 1–7) and high self-efficacy (mean = 5.12, SD = 1.79) to quit waterpipe tobacco smoking. In multivariable analysis, prior quit attempts (β = 1.10, P &lt; 0.01), greater perceived risks of waterpipe tobacco smoking (β = 0.42, P &lt; 0.01) and increasingly negative attitudes toward waterpipe tobacco smoking (β = 0.29, P &lt; 0.01) were associated with higher motivation to quit. These findings highlight the importance of those factors as potential cessation determinants. These findings can help guide the development and refinement of interventions targeting young adult waterpipe tobacco smoking.
Gender and early career status: variables of participation at an international marine science conference
Authors: Johannesen E , Barz F , Dankel D J , Kraak S B M 
Published: March 2023

Abstract
Conference participation is an important part of academic practice and contributes to building scientific careers. Investigating demographic differences in conference participation may reveal factors contributing to the continued under-representation of women in marine and ocean science. To explore the gender and career stage dimensions of participation in an international marine science conference, preferences of presentation type (oral/poster) as well as acceptance and rejection decisions were investigated using 5-years of data (2015–2019) from an International Marine Science Conference. It was found that early career scientists were more likely to be women, while established scientists were more likely to be men. Although overall, gender did not show a significant effect on the decisions to “downgrade” requests for oral presentations to poster presentations, early career scientists were significantly more likely to be downgraded than established scientists. Given that more women were often early career scientists, more women than men had their presentations downgraded. Other indicators and evidence from conference prize-giving and recognition awards point to a gender gap remaining at senior levels, highlighting the need for further actions as well as monitoring and researching conference participation from a gender perspective.
Chasing inter-species communication: what marine mammals are telling us about our oceans
Authors: Moore Sue E 
Published: March 2023

Abstract
I describe my path through a series of opportunities that provided stepping stones from childhood years in the landlocked US Midwest to a 45-year-long career focused on cetacean behaviour and ecology. My early interest in the ocean and dolphins led me to switch from majoring in journalism to biology during my undergraduate years. While pursuing a master’s degree focused on bioacoustics, I was employed as a contract scientist with the US Navy’s marine mammal laboratory. During 20 years there, my work ranged from dolphin calling behaviour to marine mammal distribution in Alaskan waters, culminating in a Ph.D. dissertation on cetacean habitats in the Alaskan Arctic. Subsequently, I enjoyed a 20-year career with the US NOAA National Marine Fisheries Service. There, I developed and advanced the idea that marine mammals can act as sentinels of ocean variability. To interpret the messages that marine mammals convey about the ocean, we must broaden science discourse to include Indigenous Knowledge and lessons from the experiences of people whose livelihoods depend on the sea. My advice to students and young professionals is to follow your passion while seeking the perspectives of colleagues from a variety of disciplines and people from all cultures and backgrounds. Coupled with a healthy dose of luck, this approach worked for me.
Total and Subgenomic RNA Viral Load in Patients Infected With SARS-CoV-2 Alpha, Delta, and Omicron Variants
Authors: Dimcheff Derek E , Blair Christopher N , Zhu Yuwei , Chappell James D , Gaglani Manjusha , McNeal Tresa , Ghamande Shekhar , Steingrub Jay S , Shapiro Nathan I , Duggal Abhijit , Busse Laurence W , Frosch Anne E P , Peltan Ithan D , Hager David N , Gong Michelle N , Exline Matthew C , Khan Akram , Wilson Jennifer G , Qadir Nida , Ginde Adit A , Douin David J , Mohr Nicholas M , Mallow Christopher , Martin Emily T , Johnson Nicholas J , Casey Jonathan D , Stubblefield William B , Gibbs Kevin W , Kwon Jennie H , Talbot H Keipp , Halasa Natasha , Grijalva Carlos G , Baughman Adrienne , Womack Kelsey N , Hart Kimberly W , Swan Sydney A , Surie Diya , Thornburg Natalie J , McMorrow Meredith L , Self Wesley H , Lauring Adam S ,   for the Investigating Respiratory Viruses in the Acutely Ill (IVY) Network
Published: March 2023

Abstract

Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomic and subgenomic RNA levels are frequently used as a correlate of infectiousness. The impact of host factors and SARS-CoV-2 lineage on RNA viral load is unclear.

Methods
Total nucleocapsid (N) and subgenomic N (sgN) RNA levels were measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in specimens from 3204 individuals hospitalized with coronavirus disease 2019 (COVID-19) at 21 hospitals. RT-qPCR cycle threshold (Ct) values were used to estimate RNA viral load. The impact of time of sampling, SARS-CoV-2 variant, age, comorbidities, vaccination, and immune status on N and sgN Ct values were evaluated using multiple linear regression.

Results
Mean Ct values at presentation for N were 24.14 (SD 4.53) for non-variants of concern, 25.15 (SD 4.33) for Alpha, 25.31 (SD 4.50) for Delta, and 26.26 (SD 4.42) for Omicron. N and sgN RNA levels varied with time since symptom onset and infecting variant but not with age, comorbidity, immune status, or vaccination. When normalized to total N RNA, sgN levels were similar across all variants.

Conclusions
RNA viral loads were similar among hospitalized adults, irrespective of infecting variant and known risk factors for severe COVID-19. Total N and subgenomic RNA N viral loads were highly correlated, suggesting that subgenomic RNA measurements add little information for the purposes of estimating infectivity.
Factors associated with COVID-19 vaccine hesitancy among healthcare workers in Cameroon and Nigeria: a web-based cross-sectional study
Authors: Aseneh Jerry Brown , Agbor Valirie Ndip , Kadia Benjamin Momo , Okolie Elvis Anyaehiechukwu , Ofomata Chinelo Janefrances , Etombi Christie Linonge , Ekaney Domin Sone M , Joko Fru Yvonne Walburga 
Published: March 2023

Abstract

Background
This study investigated the determinants of coronavirus disease 2019 (COVID-19) vaccine hesitancy among healthcare workers (HCWs) in Cameroon and Nigeria.

Methods
This analytic cross-sectional study was conducted from May to June 2021, including consenting HCWs aged ≥18 y identified using snowball sampling. Vaccine hesitancy was defined as indecisiveness or unwillingness to receive the COVID-19 vaccine. Multilevel logistic regression yielded adjusted ORs (aORs) for vaccine hesitancy.

Results
We included a total of 598 (about 60% women) participants. Little or no trust in the approved COVID-19 vaccines (aOR=2.28, 95% CI 1.24 to 4.20), lower perception of the importance of the vaccine on their personal health (5.26, 2.38 to 11.6), greater concerns about vaccine-related adverse effects (3.45, 1.83 to 6.47) and uncertainty about colleagues’ acceptability of the vaccine (2.98, 1.62 to 5.48) were associated with higher odds of vaccine hesitancy. In addition, participants with chronic disease (aOR=0.34, 95% CI 0.12 to 0.97) and higher levels of concerns about getting COVID-19 (0.40, 0.18 to 0.87) were less likely to be hesitant to receive the COVID-19 vaccine.

Conclusions
COVID-19 vaccine hesitancy among HCWs in this study was high and broadly determined by the perceived risk of COVID-19 and COVID-19 vaccines on personal health, mistrust in COVID-19 vaccines and uncertainty about colleagues’ vaccine acceptability.
Evaluating Visual Analytics for Relevant Information Retrieval in Document Collections
Authors: da Silva Sherlon Almeida , Milios Evangelos E , de Oliveira Maria Cristina F 
Published: March 2023

Abstract
Retrieving information from document collections is necessary in many contexts, e.g. researchers search for papers on a topic, physicians search for records of patients with a certain condition and police investigators seek relationships between different criminal reports. Finding relevant textual content in a corpus can be challenging in scenarios where the users expect a retrieval process with high recall. Visual Analytics (VA) systems that integrate interactive visualizations and machine learning algorithms are often advocated to support retrieval tasks in such complex scenarios. However, few studies report an end-user perspective on the utility of such systems. We present results from observational studies on VA-supported information retrieval conducted with graduate students and researchers using a system to explore collections of scientific papers. While users have, in general, positive views of the system’s potential to facilitate their retrieval tasks, some faced practical difficulties in using it effectively, and we found considerable variation in their assessment of specific functionalities. Our findings reinforce the potential of VA systems and also the importance of carefully informing users of the underlying conceptual models in such systems and their limitations.
Evaluation of a rapid combination disc test (RCDT) for direct phenotypic detection of extended-spectrum β-lactamase production inE. colifrom positive blood culture bottles
Authors: Berinson Benjamin , Degel-Brossmann Nicole , Olearo Flaminia , Roggenkamp Hannes G , Both Anna , Aepfelbacher Martin , Christner Martin , Rohde Holger 
Published: March 2023

AbstractBackgroundThe spread of multi-resistant bacteria endangers the effectiveness of empirical antimicrobial treatment, particularly in Gram-negative bloodstream infections. Thus, rapid and reliable susceptibility testing has become a key challenge of modern microbiology. Here, we evaluated a combination disc test for rapid detection of ESBL production in Escherichia coli (rapid combination disc test, RCDT) directly from blood cultures.MethodsRCDT with discs containing cefotaxime and ceftazidime alone or in combination with clavulanic acid was validated using a cryo-collection of 96 third-generation cephalosporin-resistant (3GCR), whole-genome sequenced E. coli isolates spiked into blood culture bottles. All isolates were subjected to RCDT and rapid antibiotic susceptibility testing (RAST). Zone diameters were assessed after 4, 6 and 8 h of incubation. All isolates also underwent conventional combination disc testing. The real-life performance of RCDT was assessed by analysis of 306 blood cultures growing E. coli.ResultsEighty of 90 (88.9%) ESBL-positive E. coli validation isolates were correctly identified by RCDT after 4 h of incubation. The detection rate increased to 100% after 6 and 8 h. RCDT was negative in six 3GCR E. coli isolates expressing class B or C β-lactamases. RCDT from routine blood cultures correctly classified all 56 ESBL producers and 245/250 ESBL-negative isolates after 4 h, resulting in 100% sensitivity and 98.8% specificity.ConclusionsRCDT is a reliable method for rapid ESBL detection in E. coli directly from positive blood cultures. RCDT might complement RAST to support antibiotic stewardship interventions and treatment decisions.
Health outcomes of penicillin allergy testing in children: a systematic review
Authors: Kwok Mo , Heard Katie L , May Anthony , Pilgrim Rachel , Sandoe Jonathan , Tansley Sarah , Scott Jennifer 
Published: March 2023

AbstractBackgroundPenicillin allergy labels are commonly acquired in childhood and lead to avoidance of first-line penicillin antibiotics. Understanding the health outcomes of penicillin allergy testing (PAT) can strengthen its place in antimicrobial stewardship efforts.ObjectivesTo identify and summarize the health outcomes of PAT in children.MethodsEmbase, MEDLINE, Web of Science, Cochrane Library, SCOPUS and CINAHL were searched from inception to 11 Oct 2021 (Embase and MEDLINE updated April 2022). Studies that utilized in vivo PAT in children (≤18 years old) and reported outcomes relevant to the study objectives were included.ResultsThirty-seven studies were included in the review, with a total of 8411 participants. The most commonly reported outcomes were delabelling, subsequent penicillin courses, and tolerability to penicillin courses. Ten studies had patient-reported tolerability to subsequent penicillin use, with a median 93.6% (IQR 90.3%–97.8%) of children tolerating a subsequent course of penicillins. In eight studies, a median 97.3% (IQR 96.4%–99.0%) of children were reported as ‘delabelled’ after a negative PAT without further definition. Three separate studies verified delabelling by checking electronic or primary care medical records, where 48.0%–68.3% children were delabelled. No studies reported on outcomes relating to disease burden such as antibiotic resistance, mortality, infection rates or cure rates.ConclusionsSafety and efficacy of PAT and subsequent penicillin use was the focus of existing literature. Further research is required to determine the long-term impact of delabelling penicillin allergies on disease burden.
A3 CROHN’S DISEASE PROTEOLYTIC MICROBIOTA ENHANCES INFLAMMATION THROUGH PAR2 PATHWAY IN GNOTOBIOTIC MICE
Authors: Hann A , Santiago Badenas A , Galipeau H J , Constante M , Libertucci J , Rahmani S , Jackson K , Rueda G , Rossi L , Ramachandran R , Ruf W , Caminero A , Bercik P , Verdu E F 
Published: March 2023

Abstract

Background
An imbalance in host proteases has been implicated in inflammatory bowel disease (IBD). Recent evidence implicates microbial proteolytic activity (PA) in ulcerative colitis but whether it also plays a role in Crohn’s disease (CD) remains unclear.

Purpose
We therefore investigated the colitogenic potential and underlying pathways of proteolytic CD microbiota.

Method
Adult germ-free (GF) C57BL/6 mice were colonized with CD microbiota selected based on high (CD-HPA) or low fecal proteolytic activity (CD-LPA), and from healthy controls with LPA (HC-LPA), after which total fecal proteolytic, elastolytic and mucolytic activity were analyzed in the mice. Microbial community was assessed by 16S rRNA gene sequencing. Immune function and colonic injury were investigated by inflammatory gene expression (NanoString) and histology. Colitis severity and underlying pathways were investigated in C57BL/6, Nucleotide-binding Oligomerization Domain-2 knock-out (Nod2-/-), and Protease-Activated Receptor 2 (PAR2) cleavage resistant mice (R38E-PAR2) subjected to 2% dextran sodium sulfate in drinking water for 5 days followed by 2 days on water.

Result(s)
Colonization with HC-LPA or CD-LPA lowered baseline fecal proteolytic activity compared with GF mice, which was paralleled by lower acute inflammatory cell infiltrate. CD-HPA further increased proteolytic activity compared with GF mice. Fecal supernatants from CD-LPA or HC-LPA colonized mice had lower in vitro PAR2 cleavage compared to supernatants from GF and CD-HPA colonized mice. Several genes, such as Map kinases, Rhoa, Myd88, and Tollip, were increased in GF mice compared to colonized mice. 18 genes related to inflammation and barrier function (e.g., Mapk2k6, Tnf, Claudin1) were differentially expressed between CD-LPA and CD-HPA. CD-HPA mice had lower alpha diversity, distinct microbial profiles, and higher fecal proteolytic activity compared with CD-LPA. Abundance of several beneficial species (e.g., Akkermansia muciniphilia) was decreased while other taxa were increased (e.g., Hungattella hathewayi) in CD-HPA compared to CD-LPA. H. hathewayi as well as the serine protease K04772 were transcriptionally increased in fecal samples from CD-HPA colonized mice. C57BL/6 and Nod2-/- mice, but not R38E-PAR2 mice, colonized with CD-HPA developed earlier and more severe colitis compared with mice colonized with CD-LPA.

Conclusion(s)
CD proteolytic microbiota is proinflammatory through a PAR2 pathway. H. hathewayi correlates with the proinflammatory phenotype through the serine protease K04772 in this model. The results support a role of microbial PA in CD, which could constitute a biomarker for identifying patients who would benefit from anti-proteolytic therapies.

Disclosure of Interest
None Declared