bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advanced maternal age: copy number variations and pregnancy outcomes
Authors: Cao Luoyuan , Dong Wenxu , Wu Qinjuan , Huang Xiaomin , Zeng Xiaomei , Yang Jing , Lu Jiaojiao , Chen Xunyan , Zheng Xian , Fu Xianguo 
Published: June 2023

Objective: Adverse pregnancy outcomes are closely related to advanced maternal age (AMA; age at pregnancy ≥35 years). Little research has been reported on aneuploid abnormalities and pathogenic copy number variations (CNVs) affecting pregnancy outcomes in women with AMA. The purpose of this study was to assess CNVs associated with AMA in prenatal diagnosis to determine the characteristics of pathogenic CNVs and assist with genetic counseling of women with AMA.Methods: Among 277 fetuses of women with AMA, 218 (78.7%) were isolated AMA fetuses and 59 (21.3%) were non-isolated AMA fetuses and showed ultrasound anomalies from January 2021 to October 2022. Isolated AMA was defined as AMA cases without sonographic abnormalities. Non-isolated AMA was defined as AMA cases with sonographic abnormalities such as sonographic soft markers, widening of the lateral ventricles, or extracardiac structural anomalies. The amniotic fluid cells underwent routine karyotyping followed by single nucleotide polymorphism array (SNP-array) analysis.Results: Of the 277 AMA cases, karyotype analysis identified 20 chromosomal abnormalities. As well as 12 cases of chromosomal abnormalities corresponded to routine karyotyping, the SNP array identified an additional 14 cases of CNVs with normal karyotyping results. There were five pathogenetic CNVs, seven variations of uncertain clinical significance (VOUS), and two benign CNVs. The detection rate of abnormal CNVs in non-isolated AMA cases was increasing (13/59; 22%) than in isolated AMA cases (13/218; 5.96%) (p &lt; 0.001). We also determined that pathogenic CNVs affected the rate of pregnancy termination in women with AMA.Conclusion: Aneuploid abnormalities and pathogenic CNVs affect pregnancy outcomes in women with AMA. SNP array had a higher detection rate of genetic variation than did karyotyping and is an important supplement to karyotype analysis, which enables better informed clinical consultation and clinical decision-making.
Mesenchymal stem cell-derived exosome alleviates sepsis- associated acute liver injury by suppressing MALAT1 through microRNA-26a-5p: an innovative immunopharmacological intervention and therapeutic approach for sepsis
Authors: Cai Jizhen , Tang Da , Hao Xiao , Liu Enyi , Li Wenbo , Shi Jian 
Published: June 2023

BackgroundSepsis is a syndrome with the disturbed host response to severe infection and is a major health problem worldwide. As the front line of infection defense and drug metabolism, the liver is vulnerable to infection- or drug-induced injury. Acute liver injury (ALI) is thus common in patients with sepsis and is significantly associated with poor prognosis. However, there are still few targeted drugs for the treatment of this syndrome in clinics. Recent studies have reported that mesenchymal stem cells (MSCs) show potential for the treatment of various diseases, while the molecular mechanisms remain incompletely characterized.Aims and MethodsHerein, we used cecal ligation puncture (CLP) and lipopolysaccharide (LPS) plus D-galactosamine (D-gal) as sepsis-induced ALI models to investigate the roles and mechanisms of mesenchymal stem cells (MSCs) in the treatment of ALI in sepsis.ResultsWe found that either MSCs or MSC-derived exosome significantly attenuated ALI and consequent death in sepsis. miR‐26a‐5p, a microRNA downregulated in septic mice, was replenished by MSC-derived exosome. Replenishment of miR‐26a‐5p protected against hepatocyte death and liver injury caused by sepsis through targeting Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1), a long non-coding RNA highly presented in hepatocyte and liver under sepsis and inhibiting anti-oxidant system.ConclusionTaken together, the results of the current study revealed the beneficial effects of MSC, exosome or miR-26a-5p on ALI, and determined the potential mechanisms of ALI induced by sepsis. MALAT1 would be a novel target for drug development in the treatment of this syndrome.
Deep immune B and plasma cell repertoire in non-small cell lung cancer
Authors: Patel Akshay J. , Khan Naeem , Richter Alex , Naidu Babu , Drayson Mark T. , Middleton Gary W. 
Published: June 2023

IntroductionB cells, which have long been thought to be minor players in the development of anti-tumor responses, have been implicated as key players in lung cancer pathogenesis and response to checkpoint blockade in patients with lung cancer. Enrichment of late-stage plasma and memory cells in the tumor microenvironment has been shown in lung cancer, with the plasma cell repertoire existing on a functional spectrum with suppressive phenotypes correlating with outcome. B cell dynamics may be influenced by the inflammatory microenvironment observed in smokers and between LUAD and LUSC.MethodsHere, we show through high-dimensional deep phenotyping using mass cytometry (CyTOF), next generation RNA sequencing and multispectral immunofluorescence imaging (VECTRA Polaris) that key differences exist in the B cell repertoire between tumor and circulation in paired specimens from lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC).ResultsIn addition to the current literature, this study provides insight into the in-depth description of the B cell contexture in Non-Small Cell Lung Cancer (NSCLC) with reference to broad clinico-pathological parameters based on our analysis of 56 patients. Our findings reinforce the phenomenon of B-cell trafficking from distant circulatory compartments into the tumour microenvironment (TME). The circulatory repertoire shows a predilection toward plasma and memory phenotypes in LUAD however no major differences exist between LUAD and LUSC at the level of the TME. B cell repertoire, amongst other factors, may be influenced by the inflammatory burden in the TME and circulation, that is, smokers and non-smokers. We have further clearly demonstrated that the plasma cell repertoire exists on a functional spectrum in lung cancer, and that the suppressive regulatory arm of this axis may play a significant role in determining postoperative outcomes as well as following checkpoint blockade. This will require further long-term functional correlation.ConclusionB and Plasma cell repertoire is very diverse and heterogeneous across different tissue compartments in lung cancer. Smoking status associates with key differences in the immune milieu and the consequent inflammatory microenvironment is likely responsible for the functional and phenotypic spectrum we have seen in the plasma cell and B cell repertoire in this condition.
Synthesis of graphene oxide (GO) and reduced graphene oxide (rGO) and their application as nano-fillers to improve the physical and mechanical properties of medium density fiberboard
Authors: Gul Waheed , Akbar Shah Syed Riaz , Khan Afzal , Ahmad Naveed , Ahmed Sheraz , Ain Noor , Mehmood Arshad , Salah Bashir , Ullah Syed Sajid , Khan Razaullah 
Published: June 2023

Graphene is an advanced material in the carbon group and offers greater mechanical, electrical, structural, and optical properties. Graphene oxide (GO) and reduced graphene oxide (rGO) nanoparticles were synthesized and characterized and their special effects on enhancing the physio-mechanical characteristics of medium density fiberboard (MDF) were assessed. GO and rGO nanoparticles were added to urea formaldehyde (UF) resin at different weight percentages (1.0, 2.0, and 3.0 wt%) during the dosing process. To manufacture the MDF, nanofillers were created by sonication and combination with natural wood fibers. To observe the behavior of nanoparticles in the nanofillers, microstructure characterizations were conducted. The manufactured nano MDF samples underwent physical and mechanical testing. The incorporation of GO and rGO nanoparticles into UF resin led to significant improvements in the physical and mechanical properties of the MDF. The addition of GO and rGO nanoparticles at different weight percentages (1.0, 2.0, and 3.0 wt%) resulted in a range of improvements in thickness swelling (up to 53.3% and 35.2% for GO and rGO nanoparticles, respectively), water absorption (up to 23.3% and 63.15%, respectively), and thermal conductivity (up to 42.16% and 27.7%, respectively). Additionally, the internal bond and rupture modulus of the MDF was enhanced by 59.0% and 70.0%, respectively, for GO and 41.4% and 48.5% for rGO. The highest value of the modulus of rupture (MoR) was observed at a concentration of 3.0% of rGO nanoparticles (44.7 MPa). The findings also showed that thickness swelling (Ts) and water absorption (WA) exhibited directly proportional relationships for 3.0% GO and rGO. These results suggested that incorporating GO and rGO nanoparticles into UF resin can significantly improve the physical and mechanical properties of nano MDF.
Investigating the retarding effect of CAC in alkali-activated cements
Authors: Stefanini Laura , Walkley Brant , Provis John L. 
Published: June 2023

This paper discusses the design of hybrid alkali-activated binders at ambient temperature, specifically the development of mixes based on ground granulated blast-furnace slag (GGBFS) with calcium aluminate cement (CAC) as an additive, aiming to obtain high early strength binders with optimised Al incorporation, increased crosslinking and degree of polymerisation. The effects of 10 wt% CAC replacement of GGBFS, activated with sodium silicate with varying modulus (SiO2/Na2O molar ratio) or with sodium hydroxide, on fresh and hardened properties are investigated. The inclusion of CAC in GGBFS mixtures activated using sodium silicate results in an unexpected retardation of the polycondensation reactions required to form the main calcium aluminosilicate hydrate gel phase. This is due to incomplete dissolution of the GGBFS precursor, inhibited by the rapid formation of additional reaction products (especially cubic aluminate hydrates, C3AH6) resulting in lowered reaction kinetics, and thus delayed setting and hardening times. For sodium silicate activators, this retarding effect appears to be only slightly dependent on the activator solution modulus. When activating with 4 M NaOH, the retarding effect is reduced, along with the incorporation of CAC in the C-A-S-H gel, increasing the amount of reactive aluminium present in the binder to form a more compact gel product. Increasing the molarity of NaOH solutions results in a similar delay in reaction kinetics. The results suggest the existence of only a limited pH range in which the addition of CAC can promote the formation of a binding gel with enhanced mechanical properties, whilst other factors including the effect of sodium silicate inclusion in CAC systems are still unclear. The typical hydration products of CAC were not detected in this study.
Association of TIM-3 with anterior uveitis and associated systemic immune diseases: a Mendelian randomization analysis
Authors: Lin Dan , Zhu Rong-Cheng , Tang Chun , Li Fen-Fen , Gao Mei-Ling , Wang Yu-Qin 
Published: June 2023

BackgroundWe aimed to investigate the causal association between TIM-3, an immune checkpoint inhibitor, and anterior uveitis (AU), as well as associated systemic immune diseases.Materials and methodsWe performed two-sample Mendelian randomization (MR) analyses to estimate the causal effects of TIM-3 on AU and three associated systemic diseases, namely ankylosing spondylitis (AS), Crohn's disease (CD), and ulcerative colitis (UC). Single-nucleotide polymorphisms (SNPs) associated with AU, AS, CD, and UC were selected as the outcomes: AU GWAS with 2,752 patients with acute AU accompanied with AS (cases) and 3,836 AS patients (controls), AS GWAS with 968 cases and 336,191 controls, CD GWAS with 1,032 cases and 336,127 controls, and UC GWAS with 2,439 cases and 460,494 controls. The TIM-3 dataset was used as the exposure (n = 31,684). Four MR methods, namely, inverse-variance weighting (IVW), MR-Egger regression, weighted median, and weighted mode, were used in this study. Comprehensive sensitivity analyses were conducted to estimate the robustness of identified associations and the potential impact of horizontal pleiotropy.ResultsOur studies show that TIM-3 is significantly associated with CD using the IVW method (OR = 1.001, 95% CI = 1.0002–1.0018, P-value = 0.011). We also found that TIM-3 may be a protective factor for AU although these results lacked significance (OR = 0.889, 95% CI = 0.631–1.252, P-value = 0.5). No association was observed between the genetic predisposition to particular TIM-3 and susceptibility to AS or UC in this study. No potential heterogeneities or directional pleiotropies were observed in our analyses.ConclusionAccording to our study, a small correlation was observed between TIM-3 expression and CD susceptibility. Additional studies in different ethnic backgrounds will be necessary to further explore the potential roles and mechanisms of TIM-3 in CD.
Characteristics and neurological survival following intraoperative cardiac arrest in a Swiss University Hospital: a 7-year retrospective observational cohort study
Authors: Fuchs Alexander , Franzmeier Lea , Cheseaux-Carrupt Marie , Kaempfer Martina , Disma Nicola , Pietsch Urs , Huber Markus , Riva Thomas , Greif Robert 
Published: June 2023

IntroductionLittle is known about intraoperative cardiac arrest during anesthesia care. In particular, data on characteristics of cardiac arrest and neurological survival are scarce.MethodsWe conducted a single-center retrospective observational study evaluating anesthetic procedures from January 2015 until December 2021. We included patients with an intraoperative cardiac arrest and excluded cardiac arrest outside of the operating room. The primary outcome was the return of spontaneous circulation (ROSC). Secondary outcomes were sustained ROSC over 20 min, 30-day survival, and favorable neurological outcome according to Clinical Performance Category (CPC) 1 and 2.ResultsWe screened 228,712 anesthetic procedures, 195 of which met inclusion criteria and were analyzed. The incidence of intraoperative cardiac arrest was 90 (CI 95% 78–103) in 100,000 procedures. The median age was 70.5 [60.0; 79.4] years, and two-thirds of patients (n = 135; 69.2%) were male. Most of these patients with cardiac arrest had ASA physical status IV (n = 83; 42.6%) or V (n = 47; 24.1%). Cardiac arrest occurred more frequently (n = 104; 53.1%) during emergency procedures than elective ones (n = 92; 46.9%). Initial rhythm was pre-dominantly non-shockable with pulseless electrical activity mostly. Most patients (n = 163/195, 83.6%; CI 95 77.6–88.5%) had at least one instance of ROSC. Sustained ROSC over 20 min was achieved in most patients with ROSC (n = 147/163; 90.2%). Of the 163 patients with ROSC, 111 (68.1%, CI 95 60.4–75.2%) remained alive after 30 days, and most (n = 90/111; 84.9%) had favorable neurological survival (CPC 1 and 2).ConclusionIntraoperative cardiac arrest is rare but is more likely in older patients, patients with ASA physical status ≥IV, cardiac and vascular surgery, and emergency procedures. Patients often present with pulseless electrical activity as the initial rhythm. ROSC can be achieved in most patients. Over half of the patients are alive after 30 days, most with favorable neurological outcomes, if treated immediately.
Dynamic alterations in white blood cell counts and SARS-CoV-2 shedding in saliva: an infection predictor parameter
Authors: Aghbash Parisa Shiri , Rasizadeh Reyhaneh , Shirvaliloo Milad , Nahand Javid Sadri , Baghi Hossein Bannazadeh 
Published: June 2023

IntroductionThe recent coronavirus (COVID-19) outbreak posed a global threat and quickly escalated to a pandemic. However, accurate information on potential relationships between SARS-CoV-2 shedding in body fluids, especially saliva, and white blood cell (WBC) count is limited. In the present study we investigated the potential correlation between alterations in blood cell counts and viral shedding in saliva in a cohort of COVID-19 patients.MethodIn this preliminary clinical research, 24 age-matched COVID-19 patients without comorbidities, 12 (50%) men and 12 (50%) women, were followed up for a period of 5 days to investigate whether changes in the level of viral shedding in saliva might parallel with temporal alterations in WBC count. Viral shedding in saliva was qualitatively measured by performing SARS-CoV-2 rapid antigen tests on patient saliva samples, using SARS-CoV-2 Rapid Antigen Test Kit (Roche, Basel, Switzerland). These patients were classified into two groups with sputum and non-sputum cough. WBCs counts including leukocyte (LYM), neutrophil (NEU), and LYM counts were recorded for each patient on days 1, 3, and 5.ResultsThe results of the present study showed that the levels of WBC, LYM, and NEU as well as erythrocyte sedimentation rate (ESR) increased significantly on the 5th day compared to the first day in both groups with sputum. However, the levels of C-reactive protein (CRP), Neutrophil-to-Lymphocyte Ratio (NLR) and lactate dehydrogenase (LDH) did not show significant changes.ConclusionThis study proves that investigating the change in the number of blood LYMs as well as laboratory parameters such as CRP, LDH, and ESR as biomarkers is an accurate indicator to detect the amount of viral shedding in people with sputum and non-sputum. The results of our study denote that the measured parameters exhibit the intensity of viral shedding in people with sputum.
Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure
Authors: Gao Kuo , Yu Xue , Li Fanghe , Huang Yiran , Liu Jiali , Liu Siqi , Lu Linghui , Yang Ran , Wang Chao 
Published: June 2023

IntroductionQishen Granule (QSG), a clinically approved traditional Chinese medicine, has been researched for treating heart failure (HF) for many years. However, the effect of QSG on intestinal microecology remains unconfirmed. Therefore, this study aimed to elucidate the possible mechanism of QSG regulating HF in rats based on intestinal microecological changes.MethodsA rat model with HF induced by myocardial infarction was prepared by left coronary artery ligation. Cardiac functions were assessed by echocardiography, pathological changes in the heart and ileum by hematoxylin–eosin (HE) and Masson staining, mitochondrial ultrastructure by transmission electron microscope, and gut microbiota by 16S rRNA sequencing.ResultsQSG administration improved cardiac function, tightened cardiomyocytes alignment, decreased fibrous tissue and collagen deposition, and reduced inflammatory cell infiltration. Electron microscopic observation of mitochondria revealed that QSG could arrange mitochondria neatly, reduce swelling, and improve the structural integrity of the crest. Firmicutes were the dominant component in the model group, and QSG could significantly increase the abundance of Bacteroidetes and Prevotellaceae_NK3B31_group. Furthermore, QSG significantly reduced plasma lipopolysaccharide (LPS), improved intestinal structure, and recovered barrier protection function in rats with HF.ConclusionThese results demonstrated that QSG was able to improve cardiac function by regulating intestinal microecology in rats with HF, suggesting promising therapeutic targets for HF.
Preoperative systemic immune-inflammation index may predict prolonged mechanical ventilation in patients with spontaneous basal ganglia intracerebral hemorrhage undergoing surgical operation
Authors: Xiao Huaming , Li Lei , Zhang Feng , Cheng Lei , Li Yang , Han Wenlan , Li Huanting , Fan Mingchao 
Published: June 2023

BackgroundProlonged mechanical ventilation (PMV) has been proven as a risk factor for poor prognosis in patients with neurocritical illness. Spontaneous basal ganglia intracerebral hemorrhage (ICH) is one common subtype of hemorrhagic stroke and is associated with high morbidity and mortality. The systemic immune-inflammation index (SII) is used as a novel and valuable prognostic marker for various neoplastic diseases and other critical illnesses.ObjectiveThis study aimed to analyze the predictive value of preoperative SII for PMV in patients with spontaneous basal ganglia ICH who underwent surgical operations.MethodsThis retrospective study was conducted in patients with spontaneous basal ganglia ICH who underwent surgical operations between October 2014 and June 2021. SII was calculated using the following formula: SII = platelet count × neutrophil count/lymphocyte count. Multivariate logistic regression analysis and receiver operating characteristics curve (ROC) were used to evaluate the potential risk factors of PMV after spontaneous basal ganglia ICH.ResultsA total of 271 patients were enrolled. Of these, 112 patients (47.6%) presented with PMV. Multivariate logistic regression analysis showed that preoperative GCS (OR, 0.780; 95% CI, 0.688–0.883; P &lt; 0.001), hematoma size (OR, 1.031; 95% CI, 1.016–1.047; P &lt; 0.001), lactic acid (OR, 1.431; 95% CI, 1.015–2.017; P = 0.041) and SII (OR, 1.283; 95% CI, 1.049–1.568; P = 0.015) were significant risk factors for PMV. The area under the ROC curve (AUC) of SII was 0.662 (95% CI, 0.595–0.729, P &lt; 0.001), with a cutoff value was 2,454.51.ConclusionPreoperative SII may predict PMV in patients with spontaneous basal ganglia ICH undergoing a surgical operation.
Comprehensive bibliometric research in neuroscience: focusing on ophthalmology
Authors: Xia Xiaojing , Li Lijun , Cheng Zeyu , Chen Qiyu , Huang Tao , Yu Yun , Shang Lei 
Published: June 2023

BackgroundThis study aimed to comprehensively summarize the knowledge structure and research hotspots of ophthalmology in the field of neuroscience through bibliometric and visual analysis.MethodsWe searched the Web of Science Core Collection database for articles from 2002 to 2021 related to ophthalmology in the field of neuroscience. Using VOSviewer and CiteSpace, bibliometric analysis was conducted on the number of annual ophthalmology publications, authors, organizations, countries, journals, cited references, keywords, and burst keywords.ResultsA total of 9,179 articles were published from 34,073 authors, 4,987 organizations, and 87 countries. The cited references in these articles were published in 23,054 journals. Moreover, there were 30,864 keywords among the 9,179 articles. Notably, scholars have increasingly begun paying attention to ophthalmology in the field of neuroscience in the past 20 years. Claudio Babiloni published the most articles. The University of Washington had the greatest number of articles. The United States, Germany, and England led in the number of articles published. The Journal of Neuroscience was the most cited. The article with the highest outbreak intensity was an article published by Maurizio Corbetta in Nature Reviews Neuroscience in 2002 entitled “Control of goal-directed and stimulus-driven attention in the brain.” The most important keyword was the brain, and the top burst keyword was functional connectivity.ConclusionThis study visualized ophthalmology research in the field of neuroscience through bibliometric analysis and predicted potential research trends in future to help clinicians and basic researchers provide diversified perspectives and further carry out in-depth research on ophthalmology.