bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical application of the fracture risk assessment tool in the general population and its correlation with bone turnover markers
Authors: Yang Zhi , Xuan Shu , Li Weihong , Hu Wan , Tu Ping , Duan Peng 
Published: January 2023

Objective: To compare the risk of osteoporotic fractures between the urban and urban-rural fringe populations in southern China and to explore the effect of bone turnover markers on fracture risk.Methods: Epidemiological investigations were conducted in the urban and urban-rural fringe areas of southern China in June 2018. Residents aged 40 years and over who signed informed consent forms were included. Physical examination and questionnaire collection were completed. Bone turnover markers (BTMs) including osteocalcin (OC) and beta cross-linked C-telopeptide of type 1 collagen (β-CTX) were tested. Bone mineral density (BMD) of the femoral neck and lumbar vertebrae 1–4 were measured using dual energy X-ray absorptiometry. Fracture risk assessment tool (FRAX) values were calculated to show the probability of major osteoporotic fracture (PMOF) and probability of hip fracture (PHF) over the next 10 years.Results: A total of 1,051 participants were included in this study, including 553 in the urban areas and 498 in the urban-rural fringe areas. The average PMOF and PHF were 3.4 (2.3–5.4) % and .6 (.3–1.5) %, respectively. Compared with that in the urban populations, the femoral neck BMD in the urban-rural fringe populations was lower and FRAX values were generally higher, especially for women. FRAX values in various populations were mainly negatively correlated with lumbar and femoral neck BMD and were positively correlated with β-CTX; meanwhile, only PHF was negatively correlated with OC. After adjusting for sex, elevated β-CTX levels significantly increased the risk of high PMOF in various populations and increased the risk of high PHF in the urban-rural fringe populations. In particular, the risks of increased PMOF and PHF could increase by as much as 33 times and 19.5 times, respectively, in the urban-rural fringe areas.Conclusion: The urban-rural fringe populations in Southern China may be at risk of osteoporotic fracture. In addition to being related to BMD, the FRAX value also correlates with some BTMs. Combining FRAX with BMD, and BTMs may better predict the fracture risk.
Wnt signaling pathway-derived score for predicting therapeutic resistance and tumor microenvironment in lung adenocarcinoma
Authors: Zhou Hao-min , Zhao Li-mei 
Published: January 2023

Background: Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer. Due to tumor heterogeneity, understanding the pathological mechanism of tumor progression helps to improve the diagnosis process and clinical treatment strategies of LUAD patients.Methods: The transcriptome pattern, mutant expression and complete clinical information were obtained from the cancer genome atlas (TCGA) database and microarray data from gene expression omnibus (GEO) database. Firstly, we used single sample Gene Set Enrichment Analysis (ssGSEA) to estimate the activation of Wnt signaling pathway in each sample. Consensus clustering algorithm was used to classify LUAD samples into different subgroups according to the transcription patterns of 152 Wnt signaling pathway related genes. Then, ESTIMATE, ssGSEA and Gene Set Variation Analysis (GSVA) algorithms were used to assess the biological pathways and immunocytes infiltration between different subtypes. LASSO-COX algorithm was conducted to construct prognostic model. Kaplan-Meier and multivariate Cox analysis were performed to evaluate the predictive performance of risk model. Gene features were further confirmed using external datasets. Finally, we conducted vitro assay for validating hub gene (LEF1).Results: Based on the transcription patterns of 152 Wnt signaling pathway related genes, four different subtypes of LUAD patients were screened out by consensus clustering algorithm. Subsequently, it was found that patients with cluster A and B had massive immunocytes infiltration, and the survival rate of patients with cluster B was better than that of other subgroups. According to the coefficients in the LASSO- Cox model and the transcriptome patterns of these 18 genes, the risk score was constructed for each sample. The degree of malignancy of LUAD patients with high-risk subgroup was remarkable higher than that of patients with low-risk subgroup (p &lt; 0.001). Subsequently, five top prognostic genes (AXIN1, CTNNB1, LEF1, FZD2, FZD4.) were screened, and their expression values were different between cancer and normal tissues. FZD2 and LEF1 were negatively related to ImmunoScore, and AXIN1 was negatively related to ImmunoScore. The significant correlation between LUAD patient risk score and overall survival (OS) was verified in external datasets. In the A549 cell line, knockdown of LEF1 can reduce the invasive and proliferation ability of LUAD cells.Conclusion: A innovative 18 genes predictive feature based on transcriptome pattern was found in patients with lung adenocarcinoma. These investigations further promote the insight of the prognosis of lung adenocarcinoma and may contribute to disease management at risk stratification.
Pharmaceutical administration for severe hypertension during pregnancy: Network meta-analysis
Authors: Deng Nian-Jia , Xian-Yu Chen-Yang , Han Rui-Zheng , Huang Cheng-Yang , Ma Yu-Tong , Li Hui-Jun , Gao Teng-Yu , Liu Xin , Zhang Chao 
Published: January 2023

Aims: To evaluate the efficacy of different pharmacologic treatment for severe hypertension during pregnancy.Methods: Two reviewers searched Ovid MEDLINE, Ovid EMbase, and the Cochrane Library for randomized clinical trials from the establishment of the database to 15 July 2021 that were eligible for inclusion and analyzed the pharmaceuticals used for severe hypertension in pregnancy.Results: 29 relevant trials with 2,521 participants were involved. Compared with diazoxide in rate of achieving target blood pressure, other pharmaceuticals, including epoprostenol (RR:1.58, 95%CI:1.01–2.47), hydralazine\dihydralazine (RR:1.57, 95%CI:1.07–2.31), ketanserin (RR:1.67, 95%CI:1.09–2.55), labetalol (RR:1.54, 95%CI:1.04–2.28), nifedipine (RR:1.54, 95%CI:1.04–2.29), and urapidil (RR:1.57, 95%CI:1.00–2.47), were statistically significant in the rate of achieving target blood pressure. According to the SUCRA, diazoxide showed the best therapeutic effect, followed by nicardipine, nifedipine, labetalol, and nitroglycerine. The three pharmaceuticals with the worst therapeutic effect were ketanserin, hydralazine, and urapidil. It is worth noting that the high ranking of the top two pharmaceuticals, including diazoxide and nicardipine, comes from extremely low sample sizes. Other outcomes were reported in the main text.Conclusion: This comprehensive network meta-analysis demonstrated that the nifedipine should be recommended as a strategy for blood pressure management in pregnant women with severe hypertension. Moreover, the conventional pharmaceuticals, including labetalol and hydralazine, showed limited efficacy. However, it was important to note that the instability of hydralazine reducing blood pressure and the high benefit of labetalol with high dosages intakes should also be of concern to clinicians.
Metabolomic mechanism and pharmacodynamic material basis of Buxue Yimu pills in the treatment of anaemia in women of reproductive age
Authors: Ying-ying Guo , Yan-fang Wang , Yan Deng , Su-ying Zhang , Dong Liu , Bin Luo , Xue Wang , Miao Deng , Rui-lin Ma , Xiao-hui Liu , Yu-pei Jiao , Ai-jun Sun 
Published: January 2023

Objective: To explore the pharmacological basis and mechanism of Buxue Yimu pills (BYP) in the treatment of anaemia in women from the perspective of metabolomics and network analysis.Materials and Methods: Forty-six women of reproductive age with haemoglobin 70–110 g/L were recruited. Blood samples were collected before and after 4 weeks of oral BYP treatment to assess the changes in haemoglobin, coagulation function, and iron metabolism indices. An integrated analysis of metabolomics (liquid chromatography mass spectrometry) and network analysis was performed to identify the potential pharmacodynamic mechanisms of BYP.Results: After BYP treatment, the haemoglobin level of patients significantly increased from 93.67 ± 9.77 g/L to 109.28 ± 12.62 g/L (p &lt; 0.01), while no significant changes were found in iron metabolism and coagulation-related indicators. A total of 22 differential metabolites were identified after metabolomics analysis, which were mainly related to the inhibition of inflammation and oxidative stress. Integrating pharmacodynamics and metabolomics, a network of drug-active components-targets-metabolic pathways-metabolomics was established. Acetylcholinesterase, phospholipase A2 group IIA, and phospholipase A2 group IVA may be the most promising therapeutic targets.Conclusion: BYP can inhibit inflammation and oxidative stress as well as promote haematopoiesis, potentially improving anaemia.
Natural alleles of the clock gene timeless differentially affect life-history traits in Drosophila
Authors: Andreatta Gabriele , Montagnese Sara , Costa Rodolfo 
Published: January 2023

Circadian clocks orchestrate a variety of physiological and behavioural functions within the 24-h day. These timekeeping systems have also been implicated in developmental and reproductive processes that span more (or less) than 24 h. Whether natural alleles of cardinal clock genes affect entire sets of life-history traits (i.e., reproductive arrest, developmental time, fecundity), thus providing a wider substrate for seasonal adaptation, remains unclear. Here we show that natural alleles of the timeless (tim) gene of Drosophila melanogaster, previously shown to modulate flies’ propensity to enter reproductive dormancy, differentially affect correlated traits such as early-life fecundity and developmental time. Homozygous flies expressing the shorter TIM isoform (encoded by the s-tim allele) not only show a lower dormancy incidence compared to those homozygous for ls-tim (which produce both the short and an N-terminal additional 23-residues longer TIM isoform), but also higher fecundity in the first 12 days of adult life. Moreover, s-tim homozygous flies develop faster than ls-tim homozygous flies at both warm (25°C) and cold (15°C) temperatures, with the gap being larger at 15°C. In summary, this phenotypic analysis shows that natural variants of tim affect a set of life-history traits associated with reproductive dormancy in Drosophila. We speculate that this provides further adaptive advantage in temperate regions (with seasonal changes) and propose that the underlying mechanisms might not be exclusively dependent on photoperiod, as previously suggested.
Reduction in soil CO2 efflux through alteration of hydrothermal factor in milk vetch (Astragalus sinicus L.)-rapeseed (Brassica napus L.) intercropping system
Authors: Zhou Quan , Gunina Anna , Chen Jiao , Xing Yi , Xiong Ying , Guo Zhiming , Wang Longchang 
Published: January 2023

IntroductionIntercropping has a potential to reduce the CO2 emission from farmlands. Limited information is available on the underlying reasons.MethodsThis study investigated the effect of milk vetch (Astragalus sinicus L.) (MV), rapeseed (Brassica napus L.) monoculture (RS) and intercropping (Intercrop) on soil CO2 emissions, moisture and temperature in a bucket experiment during 210 days from October 2015 to May 2016 on Chongqing, China.ResultsThe results showed that soil CO2 efflux of MV, RS and Intercrop was 1.44, 1.55 and 2.08 μmol·m-2·s-1 during seedling and stem elongation stages and 3.08, 1.59 and 1.95 μmol·m-2·s-1 during flowering and podding stages. At seeding and stem elongation stages Intercrop had 1.4 times higher soil CO2 efflux than the mean of MV and RS. In contrast, MVhad 1.6 times higher soil CO2 efflux than Intercrop thereafter, which shows it was inhibited if milk vetch presents as Intercrop only. Decreased sensitivity of soil respiration to temperature in 1.4 times and lower soil moisture by Intercrop were found compared to MV. Intercrop decreased soil moisture, especially at the seedling and stem elongation stages, compared to the monoculture. The fluctuation on soil respiration in RS and Intercrop was slight with changes in soil moisture.ConclusionThus, milk vetch-rapeseed system has a potential to decrease CO2 emission from farmland, however soil moisture should be regulated properly.
Clinical dyspnea scenario: Using high-fidelity situation simulation teaching program to evaluate learning effectiveness for clinical junior and pre-clinical nurses
Authors: Liu Yu-Hsin , Subeq Yi-Maun , Lin Po-Han 
Published: January 2023

BackgroundConfronting a patient’s breathing difficulties, clinical junior nurses often do not know how to respond, and fail to give proper evaluation and treatment. Sudden changes in the condition make the clinical nursing novices feel pressured, and even, frustrated.ObjectivesThis study aims at exploring the effectiveness of the high-realistic situational simulation of dyspnea teaching program for pre-clinical and clinical 1st year nurses after graduation.DesignThis study adopts a quasi-experimental repeated measure pre-post-test design study with nonequivalent control group pre- and post-test research design. A total of 135 subjects participated in the research: nurses, post graduate year (NPGY) (N = 69), have been employed in the adult ward of a medical center for less than 1 year; and pre-clinical nurses (N = 66), 3rd-year nursing students with nurse licenses from a university in the central part of Taiwan. Simulation-based education instructed and incorporated into the high-realistic situation simulation dyspnea teaching program. Questionnaires were used to measure the effectiveness of learning, data were analyzed with SPSS version 20.0, and the scores were repeatedly measured with the generalized estimating equation.ResultsFor “cognition, skills, attitude, self-efficacy, teamwork,” NPGY and pre-clinical nurses’ post-tests are better than pre-tests, with statistically significant results. NPGY nurses’ “skills,” “attitude” and “teamwork” learning effectiveness are better than those of the pre-clinical nurses.ConclusionThe high-realistic situational simulation of dyspnea teaching program can significantly improve the learning effectiveness of NPGY nurses and pre-clinical nurses in the clinical evaluation and treatment of dyspnea.
The effect of attire attractiveness on students’ perception of their teachers
Authors: Marici Marius , Runcan Remus , Iosim Iasmina , Haisan Alexandra 
Published: January 2023

IntroductionGenerally, people do judge a book by its cover. The purpose of this research is to investigate the effect of teachers’ attire on students’ perception of 34 psychological dimensions.MethodsThe research is an experiment, with self-reported data, in groups, based on a questionnaire. The participants were 173 students (Mage = 12.16, SD = 1.74) from Suceava, Romania. Two groups of students were asked to listen to a sample lesson of a therapeutic story, narrated by a teacher. One group was given a picture of the attractive teacher and the other group a picture of the unattractive teacher, and were told that the teacher who is narrating is the teacher in the picture. After listening to the same story, the respondents had to answer a questionnaire about teachers’ personality and characteristics.ResultsThe results indicated that when the teacher is perceived as being more attractive, the students have a greater openness for school activities, the evaluation of the teacher’s personality is more positive, the evaluation of the teaching effort is more positive, students expect a higher grade, and the perceived age of the teacher is lower.DiscussionThe article underlines the role of clothing in molding student’s perception and raises questions about dress codes in schools. Implications for school context are discussed.
E-commerce platform financing versus trade credit financing: Financing mode selection for online retailer considering live-stream selling in China
Authors: Huang Shuai , Du Bingzhi , Fan Zhi-Ping , Liu Zhixi 
Published: January 2023

IntroductionThe rise of live-stream selling has made the e-commerce platform attractive to many small and medium-sized retailers that are often faced with capital constraints. The choice between the e-commerce platform financing (EPF) and trade credit financing (TCF) for the capital-constrained e-retailers engaging in live-stream selling is particularly important problem.MethodsThis paper considers a supply chain made up of a manufacturer, an e-commerce platform that offers live-stream selling service to consumers and an online retailer with capital constraint. We, respectively, investigate the optimal decisions of the supply chain enterprises under EPF and TCF modes based on Stackelberg game models and optimization theories.ResultsWe compare the profits of supply chain firms under different cases and obtain some important conclusions through theoretical and numerical analysis.DiscussionFirst, when the e-commerce platform’s commission rate is low enough, the retailer’s ordering quantity is, under EPF mode, greater than that evidenced without capital constraint. In addition, when the retailer’s marginal profit is high and the e-commerce platform’s commission rate is low, the online retailer should choose EPF mode; in other instances, TCF is its optimal choice. Second, the e-commerce platform can obtain the highest profit under EPF mode, while TCF mode will bring the highest profit to the manufacturer. Third, when the platform’s commission rate is below a certain threshold, the profit of the entire supply chain under EPF mode is larger than that of well-funded supply chain, but TCF mode cannot. Finally, we also find there exists the access threshold about the live-stream selling. Only when the commission rate is relatively high, the e-commerce platform should offers live-stream service to consumers and the live-stream investment is the highest under EPF mode.
How responsible leadership shapes followers’ low-carbon behavior: A dual-mediation model
Authors: Zhang Yihua , Liu Xiyao , Zhang Xiaoyan 
Published: January 2023

IntroductionIn recent years, environmental problems such as global warming, rising sea levels, and species extinction have provoked a widespread concern all over the world, and many countries and international organizations have called for a reduction in carbon emissions. Theoretically, although many scholars have explored how responsible leadership influences subordinates’ work-related outcomes, little studies have examined the association between responsible leadership and employees’ low-carbon behavior. Therefore, to address this literature gap, we here drawing upon social cognitive theory developed a dual-mediation model to investigate how responsible leadership impacts employees’ low-carbon behavior, and through which mechanisms this impact may occur.MethodsBy conducting a questionnaire survey in a company in China, we collected the valid data from 411 samples. Then using SPSS 26.0 and Mplus 8.1, we tested our proposed theoretical model and hypotheses by analyzing these data.ResultsThe empirical results showed that responsible leadership was positively related to employees’ environmental consciousness, which can further increase their low-carbon behavior. At the same time, responsible leadership was negatively related to employees’ environmental apathy, which can reduce their low-carbon behavior. And employees’ environmental consciousness and environmental apathy played the mediating roles in the relationship between responsible leadership and employees’ low-carbon behavior. Furthermore, we found that leader-member exchange (LMX) magnified the direct effect of responsible leadership on employees’ environmental apathy and strengthened the indirect effect of responsible leadership on employees’ low-carbon behavior via environmental apathy, but the moderating effect of LMX on another path was not significant.DiscussionThese findings suggest that despite encouraging leaders to show responsible behaviors, promoting employees’ environmental consciousness and reducing their environmental apathy may be useful ways to facilitating their low-carbon behavior and achieving a low-carbon society. Moreover, establishing a high-quality of exchange relationship with followers may magnify the effectiveness of responsible leadership on lowering followers’ environmental apathy.
Personalized individual-based exercise prescriptions are effective in treating depressive symptoms of college students during the COVID-19: A randomized controlled trial in China
Authors: Zhao Yuanhui , Wang Wenxing , Wang Mengdie , Gao Fang , Hu Chun , Cui Bowen , Yu Wenlang , Ren Hong 
Published: January 2023

BackgroundThe COVID-19 pandemic has seriously increased depression prevalence among the public, including Chinese college students. However, many exercise cannot be performed as usual under the stay-at-home order. This study was a 12-week three-arm randomized controlled trial using the intention-to-treat principle, aiming to explore and compare the feasibility and effect of individual-based personalized aerobic-exercise and resistance-training prescriptions on depressive symptoms in college students, and conclude with some recommendations for individual-based exercise prescriptions.MethodsEighty-six college students with depressive symptoms were randomized into aerobic-exercise (AE), resistance-training (RT), and wait-list control (WLC) groups. Participants in two experimental groups received 12-week personalized AE and RT prescriptions on their individual situations, respectively. No intervention was implemented on participants in the WLC group. Depressive symptoms and physical activity (PA) were measured by Zung Self-Rating Depression Scale (SDS) and International Physical Activity Questionnaire-Short Form (IPAQ-SF), respectively. All data were collected at the baseline, 4, 8, and 12 weeks, and 4-week post-intervention.ResultsAt 12 weeks, 72.09% of depressive participants improved to “normal.” Participants exhibited a statistical reduction in SDS in all 3 groups (p &lt; 0.05) at 12 weeks compared to baseline. Follow-up assessments showed no significant increase in SDS at 4-week post-intervention compared to 12 weeks (p &gt; 0.05). The independent t-test revealed significantly lower SDS in AE and RT group than in WLC group (pAE &lt; 0.001 and pRT &lt; 0.05) at 4, 8, and 12 weeks, and 4-week post-intervention. Furthermore, the PA of participants (including total PA and intensities) in both experimental groups represented a significant improvement at 4-week post-intervention compared to baseline (p &lt; 0.05), while no differences were observed in the PA of participants in the WLC group (p &gt; 0.05).ConclusionPersonalized exercise prescriptions have good feasibility as they can increase adherence to intervention and reduce serious adverse events. Besides, individual-based personalized aerobic-exercise and resistance-training prescriptions result in a similar effect in relieving depressive symptoms and improving physical activity in college students. The individual-based exercise programs performed in 45- to 60- min with progressive moderate-to-vigorous intensity, 3 times/week for at least 12 weeks, may reduce depressive symptoms in college students during the COVID-19.