bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infralimbic Projections to the Nucleus Accumbens Shell and Amygdala Regulate the Encoding of Cocaine Extinction Learning
Authors: Nett Kelle E. , Zimbelman Alexa R. , McGregor Matthew S. , Alizo Vera Vanessa , Harris Molly R. , LaLumiere Ryan T. 
Published: January 2023

Prior evidence indicates that the infralimbic cortex (IL) mediates the ongoing inhibition of cocaine seeking following self-administration and extinction training in rats, specifically through projections to the nucleus accumbens shell (NAshell). Our own data indicate that IL activity immediately following an unreinforced lever press is critical for encoding the extinction contingencies in such procedures. Whether extinction encoding requires activity in the IL exclusively or also activity in its outputs, such as those to the NAshell and amygdala, is unknown. To address this issue, we used a closed-loop optogenetic approach in female and male Sprague Dawley rats to silence IL–NAshell or IL–amygdala activity following an unreinforced lever press during extinction training. Optical illumination (20 s) was given either immediately after a lever press or following a 20 s delay. IL–NAshell inhibition immediately following an unreinforced lever press increased lever pressing during extinction training and impaired retention of extinction learning, as assessed during subsequent extinction sessions without optical inhibition. Likewise, IL–amygdala inhibition given in the same manner impaired extinction retention during sessions without inhibition. Control experiments indicate that critical encoding of extinction learning does not require activity in these pathways beyond the initial 20 s post-lever press period, as delayed IL–NAshell and IL–amygdala inhibition had no effect on extinction learning. These results suggest that a larger network extending from the IL to the NAshell and amygdala is involved in encoding extinction contingencies following cocaine self-administration.SIGNIFICANCE STATEMENTInfralimbic cortex (IL) activity following an unreinforced lever press during extinction learning encodes the extinction of cocaine-seeking behavior. However, the larger circuitry controlling such encoding has not been investigated. Using closed-loop optogenetic pathway targeting, we found that inhibition of IL projections to the nucleus accumbens shell and to the amygdala impaired the extinction of cocaine seeking. Importantly, these effects were only observed when activity was disrupted during the first 20 s post-lever press and not when given following a 20 s delay. These findings suggest that successful cocaine extinction encoding requires activity across a larger circuit beyond simply inputs to the IL.
Studies on the use of glassionomer
Authors: Gherman Adina-Mădălina , Buduru Smaranda Dana , Mesaroș Anca-Ștefania 
Published: January 2023

Background and aims. This study is focused on the analysis of the properties of glassionomers following the choice of the type of material by the dentists. The study evaluated the properties of glassionomers used for restorations directly by comparing those prepared manually with those prepared mechanically, to find an “ideal” glassionomer and to counteract the disadvantages of the material.
Method. The study was conducted on the basis of a questionnaire and included 254 dentists from Romania. It took place between April 6 - 24, 2020 and the questionnaire was administered online. The questions focused on: the type of glassionomer chosen by every dentist, the doctor’s opinion about the properties, type and clinical indications of the material.
Results. The results showed that in Romania, the dentists use the glassionomer for a permanent filling in adults because of the financial aspect, as they stated. In their opinion the best advantages are the fluoride release and adhesion to dental tissues and, on the other hand the biggest disadvantage is aesthetics.
Conclusions. Among the conclusions are the following: the glassionomer is frequently used in dental offices, especially the powder-liquid system, as a commercial product; no standard type of glassionomer was found.
Prognostic markers for ischemic stroke – are they truly reliable?
Authors: Roman-FIlip Corina , Catană Maria-Gabriela , Mihaila Romeo-Gabriel 
Published: January 2023

Background. Stroke is one of the leading causes of mortality and morbidity worldwide. Despite extensive research, to this date there is no panel of biomarkers for the prevention and prognosis of ischemic stroke and there is still much incomplete and insufficiently researched information.
Aim. We conducted a prospective, observational study between January and June 2020. The main objective of this study was to clarify the role of inflammation markers, i.e. neutrophil/ lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), high-sensitivity C – reactive protein (hsCRP) in ischemic stroke and whether there is or not a correlation between these markers and carotid stenosis.
Study design. In the study we included 150 subjects divided in two groups: study group – 100 subjects and control group – 50 subjects.
Methods. Data collected during the research (at the time of patient admission): 1) biological sample: 5 ml of peripheral blood were collected in a vial with clot activator and separating gel, from which the following laboratory tests were performed: hsCRP, neutrophils, lymphocytes, platelets. NLR and PLR were subsequently calculated as the ratio of neutrophils to lymphocytes, respectively platelets and lymphocytes), 2) paraclinical examinations: extracranial carotid Doppler ultrasound examination.
Results. The results were impressive: high-sensitivity C reactive protein (hsCRP), neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) were strongly, respectively moderately correlated with the severity of stroke (the severity being established with the NIHS (National Institute of Health Stroke) score. None of the inflammation markers included in the present study was correlated with carotid stenosis.
Conclusion. hsCRP, NLR and PLR may potentially be prognostic markers for ischemic stroke, being of major help in preventing its possible complications.
Community-Academic Advocacy to Improve Shelter Access for Families Experiencing Homelessness
Authors: Kanak Mia , Turley Kelly , Lee Lois K. , Sandel Megan , Stewart Amanda M. 
Published: January 2023

Abstract
A 2012 Massachusetts shelter eligibility regulation required many families to spend a night in a location “not meant for human habitation” before qualifying for the state-run shelter system, and many families experiencing homelessness stayed in pediatric emergency departments (EDs) overnight to meet this requirement. ED clinicians initially recognized an increase in ED visits for homelessness after this regulation and began compiling and sharing stories with key institutional and community partners and stakeholders. To bolster advocacy efforts, the authors collected data on the magnitude of the problem and its associated health care costs. Guided by the policy knowledge of community partners, the authors leveraged the expertise and advocacy power of clinicians to share these data and stories with legislators via written and oral testimony, community events, and the media. Academic publication lent additional credibility and exposure to their research. In 2019, the Massachusetts Legislature passed budget language to overturn the 2012 shelter eligibility regulation. However, despite this policy victory, some families continue to present to EDs for homelessness. Therefore, current advocacy efforts have shifted focus toward implementation and enforcement of the new policy, monitoring issues, and developing new programmatic responses. In this advocacy case study, we illustrate how clinicians have unique abilities to serve as effective advocates for social policy change using a framework of strategies including storytelling, coalition building, tailored communication, and data sharing. Partnering with existing advocacy networks within their institution and community enhances the advocacy efforts of all stakeholders to influence social and health outcomes for children and families.
Results of the prospective observation in routine practice of hypertensive patients with concomitant chronic respiratory diseases. Part II. Predictors of adverse events
Authors: Smirnova M. I. , Gorbunov V. M. , Kurekhyan A. S. , Andreeva G. F. , Koshelyaevskaya Ya. N. , Deev А. D. 
Published: January 2023

Aim. To study the predictors of adverse events in hypertensive (HTN) patients, including those with chronic respiratory diseases (CRDs).Material and methods. Regression analysis evaluated clinical and anamnestic data and blood pressure (BP) in outpatients with stage II-III HTN (n=156): 49 patients had asthma, 20 — chronic obstructive pulmonary disease; age — 63,4±8,8 years, women — 72,4%, followup duration 29,3±8,0 months. The outcomes included in the composite primary endpoint (EP) were registered in 32 patients (death, major cardiovascular events and complications), while the secondary EPs were registered in 57 patients (hospitalizations, emergency calls, temporary disability due to cardiovascular disease/CRD, hypertensive crisis, exacerbation of CRD, pneumonia, respiratory failure), and tertiary EP — in 76 (other significant diseases/conditions, including increased blood pressure without hypertensive crisis, requiring correction of regular therapy).Results. Predictors of primary EPs were the decrease level of nocturnal diastolic BP (DBP) (β=-5,335, p=0,026), nocturnal DBP variability (β=1,796, p=0,007), blood leukocyte level (β=0,241, p=0,039); secondary EPs — systolic blood pressure ≥170 mm Hg in history (β=1,305-1,641, p=0,037-0,017), statin use (β=-0,997, p=0,037), CRD (β=0,734, p=0,023), blood platelet level (β=-0,008, p=0,022); tertiary EPs — only CRD (β=1,082, p<0,0001).Conclusion. Patients with HTN need complex multifactorial prevention. It is necessary to improve the control of office and ambulatory blood pressure, blood lipids, especially in the presence of CRD, to improve the control of CRDs themselves. The method of 24-hour blood pressure monitoring should be used more often in routine practice, including assessing the degree of nocturnal blood pressure reduction and its variability.
Long-term survival of patients with various types of cerebrovascular accident: data from the outpatient part of the REGION-M registry
Authors: Tolpygina S. N. , Zagrebelny A. V. , Chernysheva M. I. , Voronina V. P. , Kutishenko N. P. , Dmitrieva N. A. , Lerman O. V. , Lukina Yu. V. , Blagodatskikh S. V. , Lukyanov M. M. , Okshina E. Yu. , Parsadanyan N. E. , Martsevich S. Yu. , Drapkina O. M. 
Published: January 2023

Aim. To study the two-year survival rate of patients with various types of cerebrovascular accident according to the outpatient part of the REGION-M registry.Material and methods. The outpatient part of the REGION-M registry included 684 patients assigned to the City Polyclinic № 64, discharged from the F. I. Inozemtsev City Clinical Hospital (Moscow) in the period from January 1, 2012 to April 30, 2017 with a confirmed diagnosis of stroke or transient ischemic attack (TIA). Of these, 514 (75,1%) patients had an ischemic stroke, 52 (7,6%) — a hemorrhagic stroke, and 118 (17,2%) — a TIA.Results. The lowest two-year mortality (11%) was observed in patients who underwent TIA (p<0,001) compared with ischemic and hemorrhagic stroke. The relative risk of death for ischemic stroke was 2,0 (95% confidence interval (CI): 1,13-3,55) (p<0,05), and for hemorrhagic stroke — 2,51 (95% CI: 1, 22-5,15) (p<0,05) relative to patients with TIA. Mortality in patients with ischemic or hemorrhagic stroke did not differ significantly — 32,3 and 36,6% (p>0,05). The mean age of patients who underwent TIA was significantly less than the age of patients with hemorrhagic or ischemic stroke. With increasing age, the survival of patients significantly worsened in all types of strokes. In addition, multivariate analysis adjusted for age showed remained significant effect of the stroke type on survival as follows: the relative risk of death for ischemic and hemorrhagic stroke was 1,99 (95% CI: 1,12-3,53) (p<0,05) and 2,45 (95% CI: 1,19-5,02) (p<0,05).Conclusion. Mortality in patients with stroke was significantly lower after TIA than in any type of stroke and did not differ between subgroups of patients with ischemic and hemorrhagic stroke.
Comparison of endovenous laser ablation and conservative treatment in acute thrombophlebitis of the varicose great saphenous vein: rationale, design and first results of clinical trial
Authors: Kaperiz K. A. , Rastatueva A. O. , Yavelov I. S. , Drapkina O. M. 
Published: January 2023

Thrombophlebitis of the superficial lower limb veins is one of the most common complications of chronic vein disease. Nowadays a long-term (at least 45 days) subcutaneous injection of fondaparinux sodium or heparin in combination with elastic compression of the limb is recommended for treatment of superficial vein thrombosis with a moderate risk of thrombus propagation to deep venous system. However, long-term use of anticoagulants is not always possible and sometimes contraindicated. Endovenous laser ablation (EVLA) around thrombus-free junction of great or small saphenous veins without subsequent long-term use of anticoagulants seems to be a promising intervention for treatment of acute thrombophlebitis of varicose superficial veins.The prospective single-center randomized open-label clinical trial was dedicated for comparison the efficacy and safety of three following treatments options for patients with acute thrombosis of the varicose great saphenous vein and location of thrombi in the trunk and/ or its large inflows: EVLA close to saphenous vein junction without anticoagulation, EVLA and 7 days of anticoagulation and noninvasive treatment with anticoagulation for 45 days (subcutaneous injections of the fondaparinux 2,5 mg). Class 2 compression stockings are used in all patients. Adverse outcomes are recorded at 7, 45 and 90 days after randomization. These include the progression of superficial vein thrombosis, recurrent superficial vein thrombosis, thrombosis involvement of the superficial segment of the perforating vein up to the fascia, deep vein thrombosis according to compression ultrasound, and pulmonary embolism with symptoms. Bleedings according to the World Society of Thrombosis and Hemostasis and the Academic Research Consortium classification as well other complications are also recorded. The first 30 patients included in the study have a low incidence of venous thrombosis risk factors and localization of the thrombus relatively far from the saphenofemoral junction. EVLA resulted in persistent cessation of venous blood flow at the intervention site. New episodes of venous thrombosis were noted only in the group of medical treatment — deep vein thrombosis of the contralateral tibia 5 days after stopping of 45-day treatment with fondaparinux and thrombus in the recanalized inflow of the great saphenous vein without involving its trunk at the 21st day after stopping of 45-day injection of fondaparinux. There were no complications in the area of intervention and bleedings.
Nucleotide sequence variant of the TPM1 gene in a family with different phenotypes of left ventricular non-compaction
Authors: Kudryavtseva M. M. , Kiseleva A. V. , Myasnikov R. P. , Kulikova O. V. , Meshkov A. N. , Mershina E. A. , Angarsky R. K. , Sotnikova Е. A. , Divashuk M. G. , Zharikova A. A. , Koretsky S. N. , Filatova D. A. , Sinitsyn V. E. , Sdvigova N. A. , Barsky V. I. , Basargina E. N. , Drapkina O. M. 
Published: January 2023

Left ventricular non-compaction (LVNC) is a rare, genetically and phenotypically heterogeneous disease, which is often accompanied by diagnostic difficulties.Aim. To demonstrate several generations of a family with LVNC with various clinical and phenotypic manifestations of the disease (dilated and isolated types of LVNC) with an identified rs397516387 variant of the TPM1 gene.Material and methods. Based on the multicenter registry "Myocardial Non-compaction", a family with a familial form of LVNC was selected. Next generation sequencing (NGS) was performed on an Ion S5 system (Thermo Fisher Scientific, USA) using Ampliseq technology. Variant was verified using Sanger sequencing on an Applied Biosystem 3500 Genetic Analyzer (Thermo Fisher Scientific, USA). For clinical interpretation, variants in the genes associated with LVNC with a minor allele frequency <0,1% were selected in the gnomAD database (v2.1.1). Results. Variant rs397516387 was found in 5 family members, including the proband. Further examination revealed LVNC in 2 additional family members. The proband and the proband’s uncle had a dilated type of LVNC, and the proband’s mother had an isolated type.Conclusion. The paper presents several generations of a family with different phenotypic manifestations of LVNC and rs397516387 variant in the TPM1 gene. The beginning of genetic screening from a proband, a thorough collection of a family history and further detailed genetic screening of relatives led to the identification of rs397516387 variant in 4 more family members, which in turn made it possible to conduct an additional examination to confirm the diagnosis and prescribe timely drug therapy.
MARKIZ study: screening for post-COVID-19 syndrome using a questionnaire to identify symptoms and risk factors for noncommunicable diseases
Authors: Nikolaev N. A. , Drapkina O. M. , Livzan M. A. , Skirdenko Yu. P. , Gorshkov A. Yu. , Gorbenko А. V. , Drozdova L. Yu. , Andreev K. A. , Blokh A. I. , Gaus O. V. , Zakharova T. D. , Plotnikova O. V. , Fedorin M. M. 
Published: January 2023

Aim. To provide data on the diagnostic and prognostic value of questionnaire survey for post-COVID-19 syndrome during screening and regular medical check-ups of employees, as well as preventive medical examinations.Material and methods. This single-center cross-sectional comparative study involved 1120 people (92,1% of the general population — all employees of the organization), including 195 men (67,3% of the general population) and 925 women (79,5% of the general population). All participants filled out the post-COVID-19 syndrome (PS) detection questionnaire, the Hospital Anxiety and Depression Scale and the QAA-25p Adherence Assessment Questionnaire. Potential adherence to treatment was calculated using the SCOPA software. Statistical processing was performed using tools adapted for medical and biomedical research (Python language; Sklearn, NumPy, Pandas libraries) and Statistica 6.13 (StatSoft Inc., USA).Results. As a result, 47% of respondents noted at least one symptom that reduces the quality of life and/or performance efficacy. In all subsamples, women reported symptoms more frequently than men. The most informative indicators associated with post-COVID-19 syndrome are age (Mann-Whitney U test, p=0,042), number of PS symptoms (Mann-Whitney U test, p=0,001), severity of PS symptom (Mann-Whitney U test, p= 0,004) and adherence to health care (Kruskal-Wallis H test, p=0,021). An increased level of anxiety is associated with all the analyzed symptoms, depression level with 6 symptoms, age with 5 symptoms, insufficient compliance with 3 symptoms.Conclusion. PS questionnaire should be included in a comprehensive survey program of persons undergoing medical examinations. It is necessary to develop algorithms for the treatment and diagnosis of patients that take into account the number and severity of individual symptoms separately for men and women with consideration to their COVID-19 epidemiological status, as well as age and markers of anxiety, depression, and adherence to treatment.
Behaviour and material properties of versaloc semi-interlocking mortarless masonry
Authors: Dorji Sonam , Derakhshan Hossein , Thambiratnam David P. , Zahra Tatheer , Mohyeddin Alireza 
Published: January 2023

AbstractMasonry construction is popular around the world, but the use of mortared masonry presents numerous challenges. In recent decades, masonry construction systems incorporating interlocking masonry units have been proposed to eliminate mortar. The interlocking between masonry units can be achieved using specially shaped units. This paper presents a comprehensive experimental study to determine the behaviour as well as basic material properties of one such semi-interlocking mortarless masonry. The experiments included testings of ungrouted masonry units, prisms, and wallets. In addition, masonry prisms and wallets with grouted cores were also investigated to study the effect of grouting. A detailed description of the construction procedure is explained. The failure mechanisms, maximum strengths, force–displacement relationships, and modulus of elasticity of the samples are obtained and contrasted with the relevant results from the literature. Finally, recommendations are made that may assist material model development required for micro- and macro-modelling of mortarless masonry. The new information generated in this research will enable better understanding of the behaviour and properties of semi-interlocking mortarless masonry and will be useful for validating numerical models in future research.
Information Technologies: Physical and Mathematical Models of Detectors for Environment Monitoring Systems
Authors: Klyuchko Olena , Shutko Vladimir , Kolganova Olena , Lizunova Alina , Navrotskyi Denis 
Published: January 2023

Ecological monitoring is a real need in contemporary world with its growing tendency of environmental industrial pollution. Among such pollutants there are chemicals emanated in fires, explosions and other emergencies, in process of ruining of chemical enterprises, enterprises of oil and gas cycle, and etc. Some information systems for appropriate monitoring were described briefly in this article together with incorporated hybrid system of electronic chemical pollution detectors that were able to input information to such systems. In previous works of the authors the necessity of development of two types of such detectors with specified properties were substantiated: biosensors and detectors with elements – artificially elaborated selective membranes. This article describes the second type of detectors. The proposed detectors were able to detect harmful chemicals in the environment in the air and water phases; development of such devices was based on the theory of membrane systems. The detectors contained sensor elements covered with chemo-sensitive specific coatings – layers of substances: membranes, films, etc. When in contact with certain chemicals, these detectors demonstrate the properties of chemo-sensitivity and primary identification of such compounds. These studies were aimed on developing detectors of various environmental pollutants based on the theory that can be used as a basis for the detection of such substances. The works were based on a deep study of membrane systems with further development of physical and mathematical models of some substances detection taking into account basic chemical and physical properties of artificial and natural membranes, as well as their ionic selectivity. Some results of mathematical modeling of such detectors with artificial membranes – chemo-sensitive surfaces – were described in the article. Summarizing the previous experience, a brief review of authors’ publications in these items was done, as well as the works of some other authors. The purpose of the work was, basing on profound studying of artificial membrane systems, to suggest them as physical model and summarize the experience of development of their mathematical models.