bluuebunny/crossref_metadata_2025_split huggingface dataset:
MRE11A: a novel negative regulator of human DNA mismatch repair
Authors: Du Demin , Yang Yueyan , Wang Guanxiong , Chen Liying , Guan Xiaowei , Rasmussen Lene Juel , Liu Dekang 
Published: March 2023

ABSTRACTDNA mismatch repair (MMR) is a highly conserved pathway that corrects DNA replication errors. Although well characterized, MMR factors remain to be identified. As a 3’-5’ exonuclease and endonuclease, meiotic recombination 11 homolog A (MRE11A) is implicated in multiple DNA repair pathways. However, the role of MRE11A in MMR is unclear. Here, we show that MRE11A deficiency increased the sensitivity of HeLa cells to N-methyl-N’ nitro-N nitrosoguanidine (MNNG) treatment, implying a potential role of MRE11 in MMR. Moreover, we found MRE11A was largely recruited to chromatin and negatively regulated the DNA damage signals within the first cell cycle after MNNG treatment. We also showed that knockdown of MRE11A increased, while overexpressing MRE11A decreased, MMR activity in HeLa cells, suggesting that MRE11A negatively regulates MMR activity. Furthermore, we show that the recruitment of MRE11A to chromatin requires MLH1 and that MRE11A competes with PMS2 for binding to MLH1. This decreases PMS2 levels in whole cell and on chromatin, and consequently comprises MMR activity. Collectively, our findings reveal that MRE11A is a negative regulator of human MMR.
Decoding the Dual Recognition Mechanism of Glucocorticoid Receptor for DNA and RNA: Sequence vs. Shape
Authors: Johanna Hörberg , Reymer Anna 
Published: March 2023

ABSTRACTTranscription factors (TFs) regulate eukaryotic transcription through selecting DNA-binding, can also specifically interact with RNA, which may present another layer of transcriptional control. The mechanisms of the TFs-DNA recognition are often well-characterised, while the details of TFs-RNA complexation are less understood. Here we investigate the dual recognition mechanism of the glucocorticoid receptor (GR), which interacts with similar affinities with consensus DNA and diverse RNA hairpin motifs but discriminates against uniform dsRNA. Using atomic molecular dynamics simulations, we demonstrate that the GR binding to nucleic acids requires a wide and shallow groove pocket. The protein effectively moulds its binding site within DNA major groove, which enables base-specific interactions. Contrary, the GR binding has little effect on the grooves geometry of RNA systems, most notably in uniform dsRNA. Instead, a hairpin motif in RNA yields a wide and shallow major groove pocket, allowing the protein to anchor itself through nonspecific electrostatic contacts with RNA backbone. Addition of a bulge increases RNA hairpin flexibility, which leads to a greater number of GR-RNA contacts and, thus, higher affinity. Thus, the combination of structural motifs defines the GR-RNA selective binding: a recognition mechanism, which may be shared by other zinc finger TFs
Elastin-like polypeptide delivery of anti-inflammatory peptides to the brain following ischemic stroke
Authors: Howell John Aaron , Gaouette Nicholas , Lopez Mariper , Burke Stephen P. , Perkins Eddie , Bidwell Gene L. 
Published: March 2023

AbstractInflammatory processes are activated following ischemic strokes and lead to increased tissue damage for weeks following the ischemic insult, but there are no approved therapies that target this inflammation-induced secondary injury. Here, we report that SynB1-ELP-p50i, a novel protein inhibitor of the nuclear factor kappa B (NF-κB) inflammatory cascade bound to drug carrier elastin-like polypeptide (ELP), is able to enter both neurons and microglia, cross the blood-brain barrier, localize exclusively in the ischemic core and penumbra in Wistar-Kyoto and spontaneously hypertensive rats (SHRs), and reduce infarct volume in male SHRs. Additionally, in male SHRs, SynB1-ELP-p50i treatment improves survival for 14 days following stroke with no effects of toxicity or peripheral organ dysfunction. These results show high potential for ELP-delivered biologics for therapy of ischemic stroke and other central nervous system disorders and further support targeting inflammation in ischemic stroke.
Movement trajectories as a window into the dynamics of emerging neural representations
Authors: Koenig-Robert Roger , Quek Genevieve , Grootswagers Tijl , Varlet Manuel 
Published: March 2023

AbstractTransforming sensory inputs into meaningful neural representations is critical to adaptive behaviour in everyday environments. While non-invasive neuroimaging methods are the de-facto method for investigating neural representations, they remain expensive, not widely available, time-consuming, and restrictive in terms of the experimental conditions and participant populations they can be used with. Here we show that movement trajectories collected in online behavioural experiments can be used to measure the emergence and dynamics of neural representations with fine temporal resolution. By combining online computer mouse-tracking and publicly available neuroimaging (MEG and fMRI) data via Representational Similarity Analysis (RSA), we show that movement trajectories track the evolution of visual representations over time. We used a time constrained face/object categorization task on a previously published set of images containing human faces, illusory faces and objects to demonstrate that time-resolved representational structures derived from movement trajectories correlate with those derived from MEG, revealing the unfolding of category representations in comparable temporal detail (albeit delayed) to MEG. Furthermore, we show that movement-derived representational structures correlate with those derived from fMRI in most task-relevant brain areas, faces and objects selective areas in this proof of concept. Our results highlight the richness of movement trajectories and the power of the RSA framework to reveal and compare their information content, opening new avenues to better understand human perception.
The Neurochemical Mechanisms Underlying the Enhancing Effects of Rewards and Punishments on Motor Performance
Authors: Hamel R. , Pearson J. , Sifi L. , Patel D. , Hinder M.R. , Jenkinson N. , Galea J.M. 
Published: March 2023

AbstractMonetary rewards and punishments enhance motor performance and are associated with corticospinal excitability (CSE) increases within the motor cortex (M1) during movement preparation. However, such CSE changes have unclear origins; they could stem from increased glutamatergic (GLUTergic) facilitation and/or decreased type A gamma-aminobutyric acid (GABAA)-mediated inhibition within M1. To investigate this, paired-pulse transcranial magnetic stimulation was used to assess GLUTergic facilitation and GABAAinhibition within M1 whilst participants prepared to execute 4-element finger-press sequences. Behaviourally, rewards and punishments enhanced both reaction and movement times. Neurochemically, regardless of rewards or punishments, a digit-specificincrease in GLUTergic facilitation and digit-unspecificdecrease in GABAAinhibition occurred during preparation as movement onset approached. In parallel, both rewards and punishments non-specifically increased GLUTergic facilitation, but only rewards non-specifically decreased GABAAinhibition during preparation. This suggests that, to enhance performance, rewards both increase GLUTergic facilitation and decrease GABAAinhibition whilst punishments selectively increase GLUTergic facilitation. A control experiment revealed that such changes were not observed post-movement as participants processed reward and punishment feedback, indicating they were selective to movement preparation. Collectively, these results map the neurochemical changes in M1 by which incentives enhance motor performance.
The effects of continuing public sector management education: a systematic literature review
Authors: Sørensen Peter 
Published: March 2023

PurposeThe purpose of this paper is to review previous research on the effects of continuing public sector management education to systematize existing knowledge, identify gaps in the literature and to point out a need for future research.Design/methodology/approachThe purpose is realized as a systematic literature review using the Scopus and JSTOR databases as well as Google Scholar. A combination of the search words identified in previous research about the topic were used: Effect, outcome, impact and result in a combination with three different types of further adult education within leadership, management and/or administration: Master of Public Administration (MPA), Master of Public Management (MPM) and Master of Public Governance (MPG). The initial search resulted in millions of files. To continue in a manageable way, the first 10 results pages of each search were analyzed according to three categories: Individual level, organizational level and professional level.FindingsResults show a limited number of papers documenting the effects of the education programs. Most papers regard the individual level effects, very few the organizational level and a few more the professional level. Given the low number of studies on the last two levels, these are suggested for future research.Originality/valueThis is the first study to summarize knowledge on the effects of the professional masters' programs MPA, MPM and MPG.
The influence of social media celebrity endorsement on beer and wine purchase behaviour
Authors: Calvo-Porral Cristina , Rivaroli Sergio , Orosa-González Javier 
Published: March 2023

Purpose
Celebrity endorsement is one of the most popular marketing communication tools that have been used by beverage companies to influence consumers’ behaviour, but little is known about the extent this communication strategy can be impactful and benefit alcoholic beverages like beer and wine. In this context, this study aims to examine whether and how social media celebrity endorsement influences consumers’ beer and wine purchase behaviour. Further, this study examines what are the characteristics of the celebrities that exert greater influence on beer and wine purchase behaviour.

Design/methodology/approach
Based on the source credibility and source attractiveness theoretical models, and on the match-up theory, a model of consumer purchase behaviour was proposed. Online celebrity endorsement was categorised as promoting either beer or wine, as well as beer and wine brands. Then, this model is empirically analysed through multiple group structural equation modelling on two samples of consumers who read online celebrity’s recommendations in Spain (beer = 280; wine = 277).

Findings
Findings indicate that celebrity’s recommendations exert a different influence pattern on consumer purchase behaviour depending on the product category: congruence is the most relevant variable in beer endorsement, whereas expertise is the most influencing factor in wine celebrity recommendations. Therefore, beer celebrity endorsers should be congruent with the product, whereas wine endorsers should be perceived as experts.

Originality/value
This study extends the literature on celebrity endorsement providing an empirical examination of the social media celebrity characteristics that influence consumer purchase behaviour of beer and wine, reporting interesting differences between these two alcoholic beverages.
Understanding people's intention to use facial recognition services: the roles of network externality and privacy cynicism
Authors: Lyu Tu , Guo Yulin , Chen Hao 
Published: March 2023

PurposeBased on the cognition–affect–conation pattern, this study explores the factors that affect the intention to use facial recognition services (FRS). The study adopts the driving factor perspective to examine how network externalities influence FRS use intention through the mediating role of satisfaction and the barrier factor perspective to analyze how perceived privacy risk affects FRS use intention through the mediating role of privacy cynicism.Design/methodology/approachThe data collected from 478 Chinese FRS users are analyzed via partial least squares-based structural equation modeling (PLS-SEM).FindingsThe study produces the following results. (1) FRS use intention is motivated directly by the positive affective factor of satisfaction and the negative affective factor of privacy cynicism. (2) Satisfaction is affected by cognitive factors related to network externalities. Perceived complementarity and perceived compatibility, two indirect network externalities, positively affect satisfaction, whereas perceived critical mass, a direct network externality, does not significantly affect satisfaction. In addition, perceived privacy risk generates privacy cynicism. (3) Resistance to change positively moderates the relationship between privacy cynicism and intention to use FRS.Originality/valueThis study extends knowledge on people's use of FRS by exploring affect- and cognitive-based factors and finding that the affect-based factors (satisfaction and privacy cynicism) play fully mediating roles in the relationship between the cognitive-based factors and use intention. This study also expands the cognitive boundaries of FRS use by exploring the functional condition between affect-based factors and use intention, that is, the moderating role of resistance to use.
Responsible leadership practices in the hospitality sector family businesses: evidence from an emerging market
Authors: Mousa Mohamed , Arslan Ahmad 
Published: March 2023

PurposeThe study aims to find out the extent to which hospitality sector family-owned businesses in Egypt feel committed to responsible leadership practices and subsequently meet their stakeholders' expectations.Design/methodology/approachThe authors conducted semi-structured interviews with 24 owners of family restaurants in Egypt. Thematic analysis was undertaken on the collected data resulting in four major themes.FindingsThe authors find that the four dimensions (aggregation of virtuousness, stakeholder involvement, individual competencies and ethical contributions) are not fully exercised by the owners despite their readiness to behave correctly according to social norms. Furthermore, the respondents elaborated that they do not fully understand how to commence playing a role in contributing to the common good in their societies.Originality/valueTo the best of the authors’ knowledge, the present study is the first to empirically investigate responsible leadership practices in the context of small and medium-sized family businesses (restaurants in this case), particularly in the emerging market and non-Western contexts.
Customers’ willingness to choose family takaful: extending the theory of interpersonal behaviour
Authors: Mehboob Shaikh Imran , Amin Hanudin 
Published: March 2023

Purpose
This study aims to investigate the customer’s willingness to participate in family takaful using the theory of interpersonal behaviour (TIB) in Pakistan.

Design/methodology/approach
For this study, purposive sampling was used, and 310 useable questionnaires were received from the respondents who were postgraduate students, non-users of family takaful. The respondents are residing in the largest city of Pakistan, which is Karachi. The software Analysis of Moments Structures (AMOS v.25) was used to analyse the data.

Findings
The findings of this study reveal that awareness, affect, facilitating conditions and religious obligation are pivotal in determining the customers’ intention to purchase family takaful products. In addition, perceived risk and social factors are found not to be significant predictors. Resultantly, it may also be necessary to look into the factors examined in this study and other factors that may have played a great role in the acceptance of family takaful in the case of Pakistan.

Research limitations/implications
This study is limited in terms of geographic coverage as it only covers part of Karachi city as a place of investigation, and therefore, the results cannot be generalised fully. On the same note, the sampling method can also be broadened to have the actual number of respondents for generalisability purposes. Future studies may focus on the random sampling method using cluster sampling to cover other regions and provinces for a clear picture and understanding.

Originality/value
To the best of the authors’ knowledge, this work is one of the first studies to be carried out on empirical grounds using the TIB in the context of family takaful products in Pakistan.
Strategic flexibility: a systematic review and future research directions
Authors: Chanphati Jitrinee , Thosuwanchot Nongnapat 
Published: March 2023

PurposeStrategic flexibility (SF) has become an important factor for firm viability and success amidst uncertain and fast-changing environments. Firms should supplement their primary strategy with alternative ones to change courses of action whenever required. Despite these benefits, some firms are constrained by the high costs of investments. Hence, this paper aims to synthesize and systematically review extant empirical studies on SF and to provide suggestions for future research.Design/methodology/approachThe authors conducted a systematic review following the Theory, Context, Characteristics, and Methods (TCCM) framework based on 88 journal articles from 34 high-ranking publications.FindingsThe study shows that extant SF literature focuses on antecedents and outcomes and draws on three main theoretical perspectives. It also presents a growing trend of SF literature in various contexts.Originality/valueThis study provides a systematic review of SF literature from both theoretical and empirical perspectives using the TCCM framework. It highlights the significance of SF in management research and global context.
Weight-based teasing and depressive symptoms among Indian college students: exploring the moderating effect of gratitude
Authors: Bansal Suhans , Garg Naval , Singh Jagvinder 
Published: March 2023

PurposeThis instant study explores the relationship between weight-based teasing and depressive symptoms in Indian college students. It further investigates the moderating effect of gratitude on depressive symptoms occurring due to weight-based teasing.Design/methodology/approachThe study is theoretically based on Fredrickson's broaden-and-built theory (2001). PROCESS macro in IBM SPSS v21 was used to analyze the effect of gratitude in moderation of weight-based teasing and depressive symptoms. The study used correlation and regression analysis to assess the relationship between weight-based teasing and depressive symptoms.FindingsThe study has confirmed that weight-based teasing results in the development of depressive symptoms in Indian college students. The study has also revealed that gratitude casts a significant moderating effect on depression due to weight-based teasing, i.e. a reduction in regression weight of weight-based teasing.Originality/valueThis study is the first of its kind in India and will significantly add to the national literature on teasing and depression. Further, the study will help stakeholders like educators and policymakers to formulate psychological programs based on positive psychology 2.0 and gratitude to combat the rising issue of body shaming in India.
Investigating police self-legitimacy: individual-level predictors of self-identification and perceived external legitimacy
Authors: Doyle Madison K. , Roche Sean Patrick 
Published: March 2023

PurposeUsing an online survey design and a primary data collection of police officers working in a large city in the southern United States, the current study finds evidence that officers perceptions of police legitimacy can be divided into two types: self-identification and perceived external legitimacy. The study investigates the role of perceived organizational support, leader–member exchange and demographic factors in predicting perceptions of self-identification and perceived external legitimacy.Design/methodology/approachThe police legitimacy literature has focused primarily on the public's perceptions of the legitimacy of police. There is limited understanding of the components of officers' attitudes towards police legitimacy, or the predictors of those components.FindingsResults of the Ordinary Least Squares regression models indicate perceived organizational support mediates the relationship between leader–member exchange and self-identification and perceived external legitimacy. Exploratory mediation analyses indicate perceived organizational support mediates both of those relationships.Originality/valueThe results provide further evidence that the two types of self-perceived legitimacy are analytically distinct. They differ from previous work in that demographic and organizational variables predict each type similarly, and that one predictor (POS) mediates the influence of another (LMX). The results have implications for future police self-legitimacy research.
Integration of Lean manufacturing and Industry 4.0: a bibliometric analysis
Authors: Kumar Naveen , Singh Arshdeep , Gupta Sahil , Kaswan Mahender Singh , Singh Maninder 
Published: March 2023

PurposeThe purpose of this study is to identify the prominent research constituents in the domain of integration of Lean manufacturing and Industry 4.0 techniques and analyze the intellectual structure among them.Design/methodology/approachA bibliometric analysis of articles based on Donthu et al. (2021a) has been adopted to conduct a systematic review of the integration of Lean manufacturing and Industry 4.0 using the Scopus database.FindingsThe co-citation analysis and bibliographic coupling depicted three clusters and themes around which the research related to the integration of Lean manufacturing and Industry 4.0. Publications related to the topic have majorly focused on the development of conceptual models and frameworks for integrating Lean manufacturing and Industry 4.0, analyzing the compatibility between the two techniques for better implementation of one another and the techniques' combined impact on operational performance.Originality/valueMost of the review studies related to the domain of integration of Lean manufacturing and Industry 4.0 have adopted a systematic literature review methodology. The present study has tried to infer the intellectual framework of the research being conducted in the said domain using the bibliometric analysis to identify the prominent research constituents in the field and examine the intellectual relationship between them.