bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of levamisole on haematological and oxidative stress parameters in packed donkeys; Efficacy of levamisole and ivermectin against strongyle infection in donkeys
Authors: Okaiyeto Babatunde Folayemi , Bedu Sackey Anthony Kojo , Kolo Mohammed Abdullahi , Tangang Aluwong , Salisu Ibrahim 
Published: January 2023

Abstract
Stress may be caused by psychological, physiological, environmental and physical factors. The aim of the study was to investigate the ameliorative and anti-oxidative effects of levamisole on stress, and the comparative efficacy of levamisole and Ivermectin in the control of Strongyle infection in donkeys. Adult male donkeys (n = 35) aged between 4–5 years; 25 naturally infested with Strongyle spp and 10 apparently healthy (free of helminth infestation) were selected for this study, divided broadly into 2 groups: Anthelmintic efficacy study group comprising 20 (10 donkeys for Ivermectin efficacy studies and the other 10 for Levamisole efficacy study on clearing strongyles from the gastro-intestinal tract of donkeys). The oxidative stress amelioration groups were A, B, C of five donkeys each: Groups A and B donkeys were the apparently healthy group; while Group C donkeys were naturally infected with Strongyle spp. All the donkeys in the oxidative stress amelioration group participated in packing of 40 kg for 10 km. Groups B and C were treated with levamisole at 7.5 mg/kg, while Group A received no treatment prior to packing. Blood was collected from all donkeys in the oxidative stress amelioration group for haemogram and oxidative stress biomarker analyses. No significant effect (P > 0.05) was observed between groups: A, B and C for: respiratory rate, pulse rate and rectal temperature; haemogram, and malondialdehyde concentration, activities of superoxide dismutase and catalase. Erythrocyte membranes were osmotically stable at 0.5% NaCl in the treated group in comparison to the controls. Packing of donkeys for 10 km did not induce significant changes in vital parameters, haemogram and biomarkers of oxidative stress, but levamisole improved erythrocyte membrane stability. It was concluded that packing for 10 km did not induce any significant changes in blood cellular components and biomarker of oxidative stress, but levamisole improved erythrocyte membrane stability. Ivermectin was administered at a dose rate of 0.2 mg /kg per os and Levamisole at a dose rate of 7.5 mg/kg per os for the helminth efficacy study. Levamisole efficacy treatment group had a reduction test of 86.8% while Ivermectin efficacy study group had 76%. This may indicate occurrence of resistance to both drugs in the donkeys. Further research is needed in order to obtain the efficacy of this drugs in a larger population There is a need to develop better and improved management practices to combat and ameliorate the challenge that helminthosis in donkeys posses to the health and working performance of donkeys.
Radical antegrade modular pancreatosplenectomy (RAMPS) versus standard retrograde pancreatosplenectomy (SRPS) for resectable body and tail pancreatic adenocarcinoma: protocol of a multicenter, prospective, randomized phase III control trial (CSPAC-3)
Authors: Li Jialin , Shi Si , Liu Jiang , Liang Chen , Hua Jie , Meng Qingcai , Xu Hang , Wei Miaoyan , Zhang Bo , Xu Jin , Wang Wei , Yu Xianjun 
Published: January 2023

Abstract
Background

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy. Radical surgical resection offers the only potential cure. There is increasing agreement that radical antegrade modular pancreatosplenectomy (RAMPS) may benefit patients with tumors in the body and tail of the pancreas. To address this, the Chinese Study Group for Pancreatic Cancer (CSPAC) − 3 trial is proposed to compare the effect of RAMPS and standard retrograde pancreatosplenectomy (SRPS) on patient survival and preoperative safety

Methods

The randomized controlled trial will be multicenter and two-armed with blinded outcomes and intention-to-treat analysis. Three hundred patients with resectable body and tail pancreatic adenocarcinoma will be enrolled and randomly assigned to RAMPS or SRPS. Adjuvant chemotherapy based on an initial regimen will be recommended 4–6 weeks after surgery if no serious complication occurs. The hypothesis that RAMPS improves survival outcome compared with SRPS will be tested using a superiority trial. The primary outcome will be overall survival (OS). Secondary outcomes will include recurrence-free survival (RFS), R0 resection rate, the number of harvested lymph nodes, post-operative complications, and quality of life scores.

Discussion

The use of RAMPS has increased over the past decade. It is reported that RAMPS is superior to SRPS in improving both the rate of R0 resection and lymph node yield. Despite these advantages, however, there is little high-level documentation of the superiority of RAMPS in terms of survival and this needs to be investigated. To address this issue, CSPAC has instigated the first prospective, randomized phase III control trials, aiming to explore the optimal surgical strategy for improving the prognosis and OS of patients with left-sided pancreatic cancer

Trial registration

Trial registration number ChiCTR2100053844; Pre-results; Date of registration December 1, 2021
Self-assembly of CIP4 drives actin-mediated asymmetric pit-closing in clathrin-mediated endocytosis
Authors: Yu Yiming , Ozaki Takuma , Yoshimura Shige 
Published: January 2023

Abstract
Clathrin-mediated endocytosis plays a pivotal role in signal transduction pathways between the extracellular environment and the intracellular space. Accumulating evidence from live-cell imaging and super-resolution microscopy of mammalian cells suggests an asymmetric distribution of actin fibres near the clathrin-coated pit, which induces asymmetric pit-closing, rather than radial constriction. However, detailed molecular mechanisms of this “asymmetricity” remain elusive. Herein, we used high-speed atomic force microscopy to demonstrate that CIP4, a multidomain protein with a classic F-BAR domain and intrinsically disordered regions, is necessary for asymmetric pit-closing. Strong self-assembly of CIP4 via intrinsically disordered regions, together with stereospecific interactions with the curved membrane and actin-regulating proteins, generates a small actin-rich environment near the pit, which deforms the membrane and closes the pit. Our results provide a mechanistic insight into how spatio-temporal actin polymerization near the plasma membrane is promoted by a collaboration of disordered and structured domains.
Article Exploring Economic and Environmental Efficiency in Renewable Energy Utilization: A Case Study in The Organization for Economic Cooperation and Development Countries
Authors: Wang Chia-Nan , Nguyen Thi Thanh Tam , Dang Thanh-Tuan , Hsu Hsien-Pin 
Published: January 2023

Abstract
The share of renewable energy has grown significantly in global energy supply, yet the evaluation of renewable energy utilization has remained limited. Hence, this research explored economic and environmental efficiency in renewable energy utilization in 20 OECD (The Organization for Economic Cooperation and Development) countries from 2015 to 2020. The study approached the Data Envelopment Analysis - Slacks-Based Measure (DEA-SBM) to evaluate economic efficiency, and the DEA undesirable output to measure environmental efficiency. Four inputs as labor force, gross capital formation, total renewable energy capacity, and share of renewable energy were compared to two desirable outputs as the gross domestic product (GDP), and total energy production in the economic efficiency model. In the environmental efficiency model, the same inputs were evaluated with GDP as the desirable output and CO2 emissions as the undesirable output. The DEA-SBM revealed that Australia, Belgium, Norway, the United Kingdom, and the United States were efficient in economic evaluation whereas, DEA undesirable output indicated that Belgium, France, Sweden, the United Kingdom, and the United States were efficient in environmental evaluation. The governments are suggested to adjust the resources management since the number of efficient units decreased in the economic assessment while fluctuating in the environmental assessment.
Novel Compound Heterozygous CYBA Mutations Causing Neonatal-Onset
Authors: Zhang Liang , Yu Lang , Li Junshuai , Peng Yu , He Siping , Huang Ruiwen , Li Zhihui , Zhao Xiaodong 
Published: January 2023

Abstract
Chronic granulomatous disease (CGD) is a rare inborn error of immunity (IEI) characterized by a defective respiratory burst by phagocytes and defective clearance of phagocytosed microorganisms; these phenomena, caused by a defect in NADPH oxidase, result in severe and life-threatening infections in affected children. The genetically heterogeneous X-linked recessive (XL-CGD) form of GCD is caused by mutations in the CYBB gene, whereas the autosomal recessive (AR-CGD) form is caused by mutations in the CYBA, NCF1, NCF2, NCF4, or CYBC1 genes. Mutations in the CYBA gene account for a small number of CGD cases; the vast majority of these patients become symptomatic in childhood, but rarely within the first weeks of life. Here, we report a 19-day-old neonate who developed pustular rashes and invasive pulmonary aspergillosis, which was identified by a galactomannan (GM) assay of both bronchoalveolar lavage fluid (BALF) and peripheral blood samples, and by metagenomic next-generation sequencing (mNGS) of BALF. A diagnosis of CGD was based on the respiratory burst test. Detailed assessment of neutrophil activity revealed that production of reactive oxygen species (ROS) was entirely absent. Whole-exome sequencing (WES) detected a nonsense mutation (c.7G>T). In addition, copy number variation (CNV) analysis detected a novel de novomicrodeletion of 200 kb at 16q24.2-q24.3. Thus, we have identified novel compound heterozygous CYBA mutations that cause neonatal AR-CGD, thereby expanding the clinical spectrum of CYBA deficiency.
Precise Fourier series and fuzzification method analysis of Standardized thermal Energy of Solar box cooker performance: Economic, Environmental study
Authors: Bhavani Sundararajan , Nandini Karthik , Muthucumaraswamy Rajamanickam , Chithambaram Venkatesan , Shanmugan Sengottaiyan 
Published: January 2023

Abstract
A solar cooker is indispensable for preparing nutritious foodstuff than the food prepared by LPG, which is evitable. In the present scenario, the solar cooker incorporated with the coating of nanoparticles led to increased thermal performance and reduced cooking time. An attempt has been made to use the Fourier series to forecast the thermal performance and validate the Fourier series analysis of cookery liquid temperatures. Also, Fuzzification entire cookery process with the climatic parameters has been modeled logically for decision-making and to draft fuzzy rules for the cooking process. Further, during the experimentation, the cooking process has undergone a thermal analysis to validate the fuzzy inference. From the study, it is observed that both the Fourier series analysis and fuzzy logic modeling are intact with experimental observation and can be utilized for the simulation of the proposed system in various climatic conditions similar to that of the local climatic conditions in Chennai. A solar box cooker studies of environmental and economical is applied to calculate total carbon dioxide emissions (%) reduce a period when this design was utilized in contrast to Pt values that have higher dependence, which is a direct relationship with minimization of CO2 emission. The overall efficiency during boiling water is found to be 52.6% and 38.99% during the steady state temperature of the oil.
Outcome of Silicone Sling Frontalis Suspension in Isolated Uncomplicated Congenital Ptosis vs. Complicated Ptosis
Authors: Prat Daphna Landau , Zhao Cindy , Ramakrishnan Meera , Revere Karen , Katowitz William , Katowitz James 
Published: January 2023

Abstract
Objectives
To compare the outcome of silicone sling frontalis suspension (FS) surgery in children with isolated uncomplicated congenital ptosis (IUCP) vs. children with complicated ptosis.
Methods
A retrospective medical chart review of all pediatric patients who underwent silicone sling FS surgery between 2009–2020 at a single center were included. Patients were divided according to ptosis type: IUCP vs. complicated ptosis. Pre-and post-operative margin-to-reflex distance (MRD1) measurements were determined from clinical photographs. Main outcome measures were differences in improvement in eyelid height, reoperation rate, and timing between the groups.
Results
Two-hundred and eight children were included: 139 IUCP and 69 complicated cases, 83 females (40%), mean (± SD) age at intervention was 1.9 ± 2.9 years. Complicated cases included: blepharophimosis epicanthus inversus syndrome (n = 35), Marcus Gunn jaw winking (n = 12), oculomotor palsy (n = 8), congenital fibrosis of extraocular muscles (n = 3), chronic progressive external ophthalmoplegia (n = 3), and others. Mean MRD1 improved by an average of 1.6 mm in both groups. Repeat ptosis repair was performed in 50/171 (29%) patients without a history of failed ptosis procedures, and this rate was similar between IUCP and complicated cases. Children under 3 years of age had higher rates of repeat ptosis repair than older children (n = 59/175, 34% vs n = 5/33, 15%, p = 0.03 chi2).
Conclusions
Silicone sling frontalis suspension has a favorable outcome in 70% of pediatric patients. Preoperative and final MRD1 and reoperation rates were similar between both groups, suggesting that despite the higher complexity in atypical cases, the outcome is similar.
Identifying s-wave pairing symmetry in single-layer FeSe from topologically trivial edge states
Authors: Wei Zhongxu , Qin Shengshan , Ding Cui , Hu Jiangping , Sun Yu-jie , Wang Lili , Xue Qi-Kun 
Published: January 2023

Abstract
Determining the pairing symmetry of single-layer FeSe on SrTiO3 is the key to understanding the enhanced pairing mechanism; furthermore, it guides exploring new superconductors with high transition temperatures (Tc). Despite significant efforts, it remains controversial whether the symmetry is the sign-preserving s- or the sign-changed s±-wave. Here, we investigate the pairing symmetry of single-layer FeSe from a topological point of view. Using low-temperature scanning tunneling microscopy/spectroscopy, we have systematically characterized the superconducting states at isolated edges and corners of single-layer FeSe. The tunneling spectra collected at edges and corners exhibit full gap and substantial dip, respectively, demonstrating the absence of topologically non-trivial edge/corner modes. According to the theoretical calculation, these spectroscopic features are strong evidence for the sign-preserving s-wave pairing.
An integrated primary care service to reduce cardiovascular disease risk in people with severe mental illness: Primrose-A. Thematic analysis of its acceptability, feasibility, and implementation.
Authors: Shaw Philippa , Mifsud Annabel , Osborn David , Nahata Nitisha , Hall Cerdic , Prenelle Ian , Lamb Danielle 
Published: January 2023

Abstract

Background
Cardiovascular disease among patients with severe mental illness in England is a major preventable contributor to premature mortality. To address this, a nurse and peer-coach delivered service (Primrose-A) was implemented in three London general practices from 2019 (implementation continued during COVID-19). This study aimed to conduct interviews with patient and staff to determine the acceptability of, and experiences with, Primrose-A.
Methods
Eight patients who had received Primrose-A, and 3 nurses, 1 GP, and 1 peer-coach who had delivered Primrose-A in three London-based GP surgeries. Semi-structured audio-recorded interviews were conducted. Reflexive thematic analysis was used to identify themes from the transcribed interviews.
Findings
Overall, Primrose-A was viewed positively by patients and staff, with participants describing success in improving patients’ mental health, isolation, motivation, and physical health. Therapeutic relationships between staff and patients, and long regular appointments were important facilitators of patient engagement and acceptance of the intervention. Several barriers to the implementation of Primrose-A were identified, including training, administrative and communication issues, burden of time and resources, and COVID-19.
Conclusions
Intervention acceptability could be enhanced by providing longer-term continuity of care paired with more peer-coaching sessions to build positive relationships and facilitate sustained health behaviour change. Future implementation of Primrose-A or similar interventions should consider: 1) training sufficiency (covering physical and mental health, including addiction), 2) adequate staffing to deliver the intervention, 3) facilitation of clear communication pathways between staff, and 4) supporting administrative processes.
A Peptide Barcode Based Approach for Efficient in vivo Screening of RNA Delivery Systems
Authors: Odunze Uchechukwu , Rustogi Nitin , Devine Paul , Miller Lorraine , Pereira Sara , Vashist Surender , Snijder Harm , Corkill Dominic , Sabirsh Alan , Douthwaite Julie , Bond Nick , Desai Arpan 
Published: January 2023

Abstract
The clinical advancement of mRNA therapeutics for diverse applications, facilitated by lipid nanoparticles (LNPs), has demonstrated the promise of LNPs as a delivery vector. Continued exploration of LNPs for application in new disease areas requires identification and optimisation of leads in a high throughput way. Currently available high throughput in vivo delivery system screening platforms are better suited to screen for cellular uptake but less so for functional cargo delivery. We have therefore developed a Proteomics Associated LNP Screening (PALS) platform which measures functional delivery of LNPs using unique peptide ‘barcodes’. We report on the design and selection of the peptide barcodes and the evaluation of these barcodes for the screening of LNPs. We show that proteomic analysis of peptide barcodes correlates with quantification and efficacy of barcoded reporter proteins both in vitro and in vivo and, that the ranking of selected LNPs using peptide barcodes in a pool correlates with ranking using alternative methods in groups of animals treated with individual LNPs. We show that this system is sensitive, selective, and capable of reducing the size of an in vivo study by at least 10-fold, thus accelerating the discovery of new technologies for mRNA delivery.
A XGBoost predictive model of reproductive outcomes in patients following hysteroscopic adhesiolysis
Authors: Li Yazhu , Duan Hua , Wang Sha 
Published: January 2023

Abstract
Background
Infertility is the primary clinical symptom and reason for visiting patients with intrauterine adhesions (IUAs). Exploring the factors influencing the prognosis of IUAs and establishing a predictive model for reproductive outcomes after hysteroscopic adhesiolysis (HA) are extremely important for the selection of clinical treatment and prognostic assessment.
Methods
The clinical informations of 369 individuals diagnosed with and treated for IUAs were obtained from the Intrauterine Adhesion Multicenter Prospective Clinical Database (IUADB, NCT05381376) and randomly divided into the training and validation cohorts. A univariate analysis was performed to identify relevant clinical indicators, followed by a least absolute shrinkage and selection operator (LASSO) regression for regularization and SHapley Additive exPlanation (SHAP) for extreme gradient boosting (XGBoost) predictive model visualization. Finally, receiver operating characteristic (ROC) curves were constructed to assess the model’s efficiency.
Results
Univariate analysis and LASSO regression demonstrated that 12 clinical indicators were significantly associated with postoperative reproductive outcomes in IUAs patients. SHAP visualization indicated that postoperative fallopian tube ostia, blood supply, uterine cavity shape and age had the highest significance. The area under the ROC curve (AUC) of the XGBoost model in the training and validation cohorts was 0.987 (95% CI 0.9787–0.996) and 0.9851 (95% CI 0.9668-1), respectively. These values were significantly higher than those of the American Fertility Society (AFS) classification, the Chinese Society for Gynaecological Endoscopy (CSGE) classification and endometrial thickness (all P < 0.01).
Conclusions
The XGBoost model had higher accuracy in predicting postoperative reproductive outcomes in IUAs patients. Clinically, our model may be useful for managing and categorizing IUAs and determining optimal action to aid in pregnancy.
Trial registration:
The study was an observational cohort study, and the data were obtained from the Chinese Uterine Adhesion Database (ClinicalTrials.gov; NCT05381376; 19/05/2022).
A fabricated hydrogel of hyaluronic acid / curcumin shows super-activity to heal the bacterial infected wound
Authors: Khaleghi Maryam , Haghi Fakhri , Gholami Mina , Hoorfar Hamdam , Shahi Farshad , Zekoloujeh Ali Mir Mousavi , Aliakbari Farhang , Ahmadi Ebrahim , Morshedi Dina 
Published: January 2023

Abstract
High risk of acute morbidities and even mortality from expanding the antibiotics resistant infectious wounds force indefinite efforts for development of high performance wound-healing materials. Herein, we design a procedure to fabricate a hyaluronic acid (HA)-based hydrogel to conjugate curcumin (Gel-H.P.Cur). The highlight of this work is to provide a favorite condition for capturing curcumin with protecting its structure and intensify its activities because of the synchronization with HA. Accordingly, HA as a major component of dermis with critical role in establishing skin health, could fortify the wound healing property as well as antibacterial activity of the hydrogel. Gel-H.P.Cur showed antibacterial properties against Pseudomonas aeruginosa (P. aeruginosa), which were examined by bactericidal efficiency, disk diffusion, anti-biofilm, and pyocyanin production assays. The effects of Gel-H.P.Cur on the inhibition of quorum sensing (QS) regulatory genes that contribute to expanding bacteria in the injured place was also significant. In addition, Gel-H.P.Cur showed high potential to heal the cutaneous wounds on the mouse excisional wound model with repairing histopathological damages rapidly and without scar. Taken together, the results strongly support Gel-H.P.Cur as a multipotent biomaterial for medical applications regarding the treatment of chronic, infected, and dehiscent wounds.