bluuebunny/crossref_metadata_2025_split huggingface dataset:
A technique for passing tuberosity-implant cerclage ribbons in fracture-inverse total shoulder arthroplasty.
Authors: Tanagho Andy , Hagnasir Ahmed Shawgi 
Published: April 2023

Abstract
Complex proximal humerus fractures are difficult to treat particularly in patients above 65 years with osteoporotic bone and disturbed anatomical landmarks. Fracture-inverse total shoulder arthroplasty has shown a good treatment option for these injuries. However better results are associated with meticulous reduction and fixation of the tuberosities around the implant. The purposeof this study to describe a simple technique to pass cerclage ribbons to fix the tuberosities together around the inverse total shoulder arthroplasty.
Methods: This is a retrospective cohort study which was performed from January 2019 to January 2021. Thirty-five shoulders with 3- & 4-parts proximal humerus fractures had undergone fracture-inverse total shoulder arthroplasty with a special technique for tuberosities repair. Follow up was minimum 24 months.
Results: The tuberosity healing rate was 86%. Complete resorption of one or more tuberosities occurred in 8%, and tuberosity migration and nonunion presented in 6%.
Conclusion:This surgical technique offers a simple way for passing cerclage ribbons in Fracture-inverse total shoulder arthroplasty for fixing the tuberosities together and to the implant. It has been shown to encourage tuberosity healing which improves shoulder stability and results in more optimal restoration of rotator cuff function.
The identification of eIF4E as the causal host factor for Sugarcane streak mosaic virus resistance
Authors: Shan Hongli , Chen Du , Zhang Rongyue , Wang Xiaoyan , Li Jie , Wang Changmi , Li Yinghu , Li Wenfeng , Wei Zhaoxia , Huang Yingkun 
Published: April 2023

Abstract
Sugarcane mosaic disease, mainly caused by Sugarcane streak mosaic virus (SCSMV), has serious adverse effects on the yield and quality. Eukaryotic translation initiation factor 4E (eIF4E) is a natural resistance gene in plants. The eIF4E-mediated natural recessive resistance comes from non-synonymous mutations of the eIF4E protein. In this study, two sugarcane varieties (CP94-1100 and ROC22) with different resistance to SCSMV were selected for the analysis of the differences in sugarcane resistance. The result showed that the nucleotide and amino acid sequences of the CP and VPg of SCSMV an between varieties were highly consistent and belong to the same isolate. However, four base missense mutations in the ORF region of eIF4E resulted in different conserved domains. Therefore, the difference in resistance comes from the differences in the eIF4E gene of the sugarcane varieties themselves. The coding region of eIF4E gene were 28 SNP loci, no InDel loci, and affected by negative selection, and was relatively conserved. A total of 11 haploids encoded 11 protein sequences. Protein spatial structure prediction revealed three amino acid non-synonymous mutations sites located in the cap pocket of eIF4E, among which one site only existed in a resistant material (Yuetang 55) and one site only existed in a susceptible material (ROC22), suggesting that the two sites might be related to the resistance to SCSMV. These results provide a strong basis for further analysis of the functional role of eIF4E in regulating mosaic resistance in sugarcane and developing resistance varieties.
Accelerated Blood Vessel Enhancement in Retinal Fundus Image based on Reconfigurable Hardware
Authors: Wang Yuyao 
Published: April 2023

Abstract
Retinal blood vessel extraction and enhancement is an intensively researched topic as it is an irreplaceable component in ocular disease screening systems. The matched filter method has been proven superior for blood vessel extraction and enhancement compared to edge detection algorithms in that it can extract blood vessel along its path and retain blood vessel depth information even when blood vessels are prone to be indistinguishable from the background. This work has implemented matched filter method with Verilog Hardware Description Language, taking advantage of the highly customizable feature and parallel computation capabilities by FPGA. The first proposed design method employs an innovative resource-efficient technique based on matched filter technique. It can be applied to situations where budget and physical resource is limited. The second design method is a time-efficient processing technique that provides further improvement in that it eliminates the gap incurred in convolution between two rows of data. As has been verified via simulation, it can offer a continuous output with about 9% increase in processing speed for conducted simulation compared to the first proposed technique.
Prognostic Importance of Glycemic Variability on Left Ventricular Reverse Remodeling after the first episode of ST-segment elevation myocardial infarction
Authors: Hanajima Yohei , Iwahashi Noriaki , Kirigaya Jin , Horii Mutsuo , Minamimoto Yugo , Gohbara Masaomi , Abe Takeru , Okada Kozo , Matsuzawa Yasushi , Kosuge Masami , Ebina Toshiaki , Hibi Kiyoshi 
Published: April 2023

Abstract
Background: This study aimed to investigate the effect of glycemic variability (GV), determined using a continuous glucose monitoring system (CGMS), on left ventricular reverse remodeling (LVRR) after ST-segment elevation myocardial infarction (STEMI).
Methods: A total of 201 consecutive patients with STEMI who underwent reperfusion therapy within 12 hours of onset were enrolled. GV was measured using a CGMS and determined as the mean amplitude of glycemic excursion (MAGE). Left ventricular volumetric parameters were measured using cardiac magnetic resonance imaging (CMRI). LVRR was defined as an absolute decrease in the LV end-systolic volume index of > 10% from 1 week to 7 months after admission. Associations were also examined between GV and LVRR and between LVRR and the incidence of major adverse cardiovascular events (MACE; cardiovascular death, acute coronary syndrome recurrence, non-fatal stroke, and heart failure hospitalization).
Results: The prevalence of LVRR was 28% (n = 57). The MAGE was independent predictor of LVRR (odds ratio [OR] 0.98, p = 0.002). Twenty patients experienced MACE during the follow-up period (median, 65 months). The incidence of MACE was lower in patients with LVRR than in those without (2% vs. 13%, p = 0.016).
Conclusion: Low GV, determined using a CGMS, was significantly associated with LVRR, which might lead to a good prognosis. Further studies are needed to validate the importance of GV in LVRR in patients with STEMI.
A study to assess the applicability of using remote sensing to minimize service interruption of Canadian port infrastructure
Authors: Holden Heather , Abdallah Maha Hussein , Rowlands Dane 
Published: April 2023

Abstract
Remote sensing can be an effective tool for providing early warning of deformations that are difficult or impossible to detect with visual inspections, and can allow infrastructure owners and operators to prioritize key areas for maintenance and assist in more rapid recovery from incidents that cause service interruptions. Canadian Port Authorities (CPAs) do not currently use remote sensing techniques to assess the condition of critical infrastructure despite their application to similar operations such as bridges, pipelines, and dams. Interviews with key informants indicate that there are no technical or scientific barriers to using remote sensing to assess port conditions, and that while the presence of heterogeneous materials and surfaces, restricted air space, and a complex web of interconnected port partners and tenants complicates its use, these are surmountable. Instead, interviews suggest that the primary barrier stopping CPAs from implementing remote sensing is that there is no system-wide formal chain of responsibility and authority and a lack of public sector agency leadership resulting from the commonly used “landlord model” for port operations, which generates fragmented stakeholder interests and inhibits collaboration. Given the emerging challenges of aging infrastructure, climate change-related impacts, and the demands of system-wide business continuity, we argue that a private and public sector collaborative pilot project is needed to test the feasibility of adopting remote sensing for port infrastructure assessment.
Arsenic Trioxide Synergistically Enhances the Anti-Neoplastic Effect of Gemcitabine on Breast Cancer Cells by Promoting Mitochondrial Dysfunction
Authors: Maleki Farshid , Handali Somayeh , Rezaei Mohsen 
Published: April 2023

Abstract
Breast cancer is the most common type of cancer in women worldwide. A common approach to cancer treatment in clinical practice is to use a combination of drugs to enhance the anticancer activity of drugs while reducing their side effects. In this regard, we evaluated the effectiveness of combined treatment with gemcitabine (GCB) and arsenic (ATO) and how they affect the cell death pathway in cancer cells. Cytotoxic activity of drugs individually or combined against MDA-MB-231 and MCF-7 was performed by MTT method and isobolographic analysis was used to determine the interaction between these factors. The combination of ATO and GCB showed synergistic anti-cancer activity (CI < 1) in both cancer cell lines. The combination of ATO and GCB induced sub-G1 phase arrest, apoptosis and death rates in MCF-7 and MDA-MB-231 cells. The apoptotic response induced by the combination of GCB and ATO was dependent on caspase 3/7. Combined treatment with MMP reduction and increased ROS production caused mitochondrial dysfunction. Co-treatment significantly reduced CAT activity in both cancer cells compared to the control group and cells treated with each monotherapy. A significant decrease in cellular GSH was observed in cancer cells treated with ATO and GCB. In addition, migration and invasion were significantly reduced in breast cancer cells treated with the combination of ATO and GCB compared to cells treated with ATO and GCB. In conclusion, the combined treatment of ATO and GCB synergistically increased the anti-cancer activity, and these findings provide an effective approach for the treatment of breast cancer. To the best of our knowledge, this is the first study showing promising results for combination therapy with ATO and GCB in breast cancer.
Integrated Analysis of the Roles of Oxidative stress related genes and Prognostic Value in Clear Cell Renal Cell Carcinoma
Authors: Wang Danwen , Deng Zhao , Lu Mengxin , Deng Kai , Li Zhiqiang , Zhou Fenfang 
Published: April 2023

Abstract
Background：As the most common diagnosed subtype in renal cell carcinoma, clear cell renal cell carcinoma (ccRCC) patients suffer from the threat of tumor metastasis and recrudesce. Previous research has established that oxidative stress could induce tumorigenesis in many cancers and can be a cancer therapeutic target. Despite these, little progress has been made in the association of oxidative stress related genes (OSRGs) with ccRCC.
Methods：MTT survival assay, qRT-PCR, apoptosis assay, cell cycle assay, ROS assay, IHC staining, were used in vitro experiments.
Results：In our study, 12 differentially expressed oxidative stress-related genes (DEOSGs) and related transcription factors (TFs) relevant to overall survival (OS) were screened, as well as their mutual regulatory networks were structured by data from the TCGA database. Moreover, we constructed the risk model of the OSRGs, and performed clinical prognostic analysis and validation. Next, we correlated MELK, PYCR1, and PML with immune infiltration in ccRCC. Tissue microarray also verified the high expression of MELK and PYCR1 in ccRCC. Finally, cellular experiment in vitro demonstrated that knockdown of MELK or PYCR1 significantly inhibited ccRCC cell proliferation by causing cell apoptosis and inducing G1 phase cycle arrest. The intracellular ROS levels were elevated after knockdown of the two genes.
Consulsion: Our results presented a potential application of DEORGs in prognostic prediction for ccRCC and identified two biomarkers named PYCR1 and MELK, which could regulate the proliferation of ccRCC by affecting the ROS levels. Further, PYCR1 and MELK could be promising to predict the progression and prognosis of ccRCC, thereby serving as new targets for medical treatments.
Chronic disease and multimorbidity in the Chinese older adults’ population and their impact on daily living ability: A cross-sectional study of the Chinese Longitudinal Healthy Longevity Survey (CLHLS)
Authors: Chen Ye , Ji Huixia , Shen Yang , Liu Dandan 
Published: April 2023

Abstract
Background
Owing to an increase in life expectancy, it is common for the older adults to suffer from chronic diseases that can result in disability and a low quality of life. This study aimed to explore the influence of chronic diseases and comorbidities on activities of daily living (ADLs) and instrumental ADLs (IADLs) in an older Chinese populations.
Methods
Based on the Chinese Longitudinal Healthy Longevity Survey (2018), 11,068 older adults aged 65 years and above were included in the study. A self-administered questionnaire was used to collect information on demographic characteristics, chronic diseases, ADLs, and IADLs. The impact of factors affecting ADL and IADL impairment in older adults was analysed using binary logistic regression.
Results
In total, 64.8% participants had chronic diseases. Hypertension, heart disease, arthritis, and cerebrovascular disease were among the top chronic diseases. Of these, 32.1% participants had multimorbidities. The most common combination of the two chronic diseases was hypertension and heart disease (10.5%), whereas the most common combination of the three chronic diseases was hypertension, heart disease, and diabetes (2.87%). After categorising the older adults into four age groups, dementia, Parkinson's disease, respiratory disease, visual impairment, and hearing impairment were found to be more prevalent with increasing age. The prevalence of hypertension, heart disease, cerebrovascular disease, gastrointestinal ulcers, and arthritis gradually increased with age until the age of 75 years, peaked in the 75–84 years age group, and then showed a decreasing trend with age. The prevalence of chronic diseases and multimorbidities followed similar trends. Regression analysis indicated that physical activity, educational background, number of chronic diseases, and age were the most significant factors influencing ADLs and IADLs. In contrast, obesity and underweight status were linked to increased risks of impaired ADLs and IADLs, as referenced by normal body mass index.
Conclusion
Chronic diseases and multimorbidities are common in older adults, and it is important to note that aging, multimorbidity, obesity, underweight, and unhealthy lifestyle choices may interfere with ADLs in older adults. Therefore, it is imperative that primary healthcare providers pay special attention to older adults and improve screening for comorbidities and follow-up needs.
From the West to the East: an evidence-based educational reform for modern medical students in traditional Chinese medicine learning
Authors: Liu Zhenrui , Ren Zhixuan , Fang Linhan , Liao Yuxuan , Ren Dan , Yu Yao , Qin Yixuan , Wu Jianzhen , Rong Pengfei 
Published: April 2023

Abstract
Generally, traditional Chinese medicine (TCM) courses are now given to modern medicine students without proper course scheduling, resulting in poor teaching results. To analyze the main factors affecting TCM learning, we surveyed the medical students and TCM teachers from Xiangya School of Medicine of Central South University via online questionnaires. The questionnaire comprised two parts, the students’ part included the basic information, the subjective cognition in TCM, the attitude towards TCM course arrangements, and the attitude towards curriculum content and the design of TCM. The teachers’ part included the basic information, the attitudes and opinions on TCM course arrangements, and suggestions and views on TCM teaching reform. The related data were collected from 187 medical students divided into two groups, namely, clinical medical students and non-clinical medical students. We found a more positive attitude toward TCM [including “Scientific nature of TCM” (P = 0.03) and “Necessity for modern medicine students to learn TCM” (P = 0.037)] in clinical medical students compared with non-clinical medical students, clinical and non-clinical medical students tended to find TCM courses difficult, and the students prefer clinical training to be better than theoretical teaching, while the teachers believe that lecture-based education should have a more significant proportion. Hence, to optimize the current TCM teaching, we conducted education reform, including differentiated teaching, hybrid teaching, and selective teaching.
Associations of a Plant-centered Diet and Lung Function Decline across Early to Mid-Adulthood: The CARDIA Lung Study
Authors: Wharton Robert , Wang Jing Gennie , Choi Yuni , Eisenberg Elliot , Jackson Mariah K. , Hanson Corrine , Liu Bian , Washko George R. , Kalhan Ravi , Jacobs David R. , Bose Sonali 
Published: April 2023

Abstract
Background Lung function throughout adulthood predicts morbidity and mortality even among adults without chronic respiratory disease. Diet quality may represent a modifiable risk factor for lung function impairment later in life. We investigated associations between nutritionally-rich plant-centered diet and lung function decline across early and middle adulthood from the Coronary Artery Risk Development in Young Adults (CARDIA) Study.Methods Diet was assessed at baseline and years 7 and 20 of follow-up using the validated CARDIA diet history questionnaire. Plant-centered diet quality was scored using the validated A Priori Diet Quality Score (APDQS), which weights food groups to measure adherence to a nutritionally-rich plant-centered diet 1 to 5 points for 20 beneficially rated foods and 5 to 1 points for 13 adversely rated foods. Scores were cumulatively averaged over follow-up and categorized into quintiles. The primary outcome was lung function decline, including forced expiratory volume in 1 second (FEV1) and functional vital capacity (FVC), measured at years 0, 2, 5, 10, 20, and 30. We estimated the association of APDQS with annual pulmonary function changes in a repeated measures regression model, adjusting for clinically relevant covariates.Results The study included 3,787 Black and White men and women aged 18–30 in 1985-86 and followed for 30 years. In multivariable repeated measures regression models, individuals in the lowest APDQS quintile (poorest diet) had declines in FEV1 that were 1.6 ml/year greater than individuals in the highest quintile (35.0 vs. 33.4 ml/year, ß±SE per 1 SD change APDQS 0.94 ± 0.36, p = 0.009). Additionally, declines in FVC were 2.4 ml/year greater in the lowest APDQS quintile than those in the highest quintile (37.0 vs 34.6 ml/year, ß±SE per 1 SD change APDQS 1.71 ± 0.46, p < 0.001). The association was not different between never and ever smokers (pint = 0.07 for FVC and 0.32 for FEV1). In sensitivity analyses where current asthma diagnosis and cardiorespiratory fitness were further adjusted, results remained similar.Conclusions In this 30-year longitudinal cohort study, long-term adherence to a nutritionally-rich plant-centered diet was associated with slower decline in lung function, highlighting diet quality as a potential treatable trait supporting long-term lung health.
Alteromonas salexigens sp.nov., isolated from coastal seawater
Authors: Sun Liping , Hu Xinyuan , Wang Qin , Niu Huijing , Pei Caixia , Li Yi , Xia Chengqiang 
Published: April 2023

Abstract
A Gram-negative, aerobic, short rod-shaped bacterium, designated ASW11-19T, was isolated from a coastal seawater sample of the Yellow Sea, PR China. Strain ASW11-19T grew optimally at 37°C, 3.0–5.0% (w/v) NaCl and pH 7.5. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain ASW11-19T belonged to the genus Alteromonas and most closely related to Alteromonas profundi 345S023T and Alteromonas fortis 1T (98.4%, both). The draft genome was 3.55 Mb with 3150 protein-coding genes, 18 contigs, and a DNA G + C content was 44.4%. The digital DNA–DNA hybridization and average nucleotide identity values were below the species-delineating thresholds. The major fatty acids were summed featured 3 (C16:1ω7c/C16:1ω6c), summed featured 8 (C18:1ω7c/C18:1ω6c) and C16:0. The sole respiratory quinone was ubiquinone 8. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phospholipid, and two unidentified lipids. Based on these genomic data, phenotypic and chemotaxonomic properties, strain ASW11-19T is considered to represent a novel species of the genus Alteromonas. The name Alteromonas salexigens sp.nov. is proposed for ASW11-19T (= MCCC 1K07239T = KCTC 92247T).
A Treatment of a Multistep Collocation Method for the Direct Solution of Second-order Odes Using a Class of Modified Bdf-type
Authors: Tumba Pius , Atsi Kaze , Suleiman Enoch 
Published: April 2023

Abstract
A research has been conducted at the Department of Mathematics, Faculty of Science, Federal University, Gashua, Yobe State to study the treatment of multistep collocation method for the direct solution of second order ordinary differential equations using a class of modified BDF-type with one super-future point. The research has proposed the construction of a new method of solving second-order initial valued problem of ordinary differential equation. A step-number, k = 3, a number of discrete members are obtained and used in block through multi-step collocation approach. The stability properties of the newly to be constructed methods are investigated using written computer codes and its convergence are established. The numerical efficiency of the method has been tested on some treated second-order initial valued problems, in order to ascertain its suitability. The solutions of the problems are compared with the corresponding exact solutions and the associated absolute errors are presented. Tables and graph have been adopted in the presentation of results. Conclusion and recommendation are made for further investigations.