bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physicochemical properties of Kakol (Suaeda aegyptiaca) essential oil nanoemulsion and its effect on the storage quality of rainbow trout (Oncorhynchus mykiss) during cold storage
Authors: Zibaee Payam , Shamekhi Mohammad‐Amin 
Published: June 2023

AbstractThe study aims to analyze the chemical composition of Suaeda aegyptiaca essential oil (PSAE) by GC–MS, produce the nanoemulsified essential oil (NSAE) using ultrasound, and compare the antimicrobial and antioxidant activity of the PSAE and NSAE in laboratory medium and rainbow trout fish (Oncorhynchus mykiss). Geranyl‐acetone (30.52%) and p‐Vinylguaiacol (10.66%), and (e)‐β‐ionone (7.79%) were the main PSAE chemical compounds. The mean droplet size diameter, polydispersity index, and viscosity of NSAE were 179.67 nm, 0.255, and 0.96 cP, respectively. PSAE and NSAE showed a moderate antiradical potential against DPPH‐ and ABTS‐free radicals (50 < IC50 < 250 μg mL−1). There was no significant difference between antiradical scavenging of PSAE and NSAE (p > .05). E. faecalis and K. pneumonia were the most and lowest sensitive bacteria to PSAE and NSAE, respectively. Examining different treatments on the shelf‐life of minced fish showed that Kakol essential oil could improve the shelf‐life of fish between 12.5% and 60% (depending on quality index). There was no significant difference between the bioactivity of PSAE and NSAE, which means that the nanoemulsion showed acceptable performance at lower essential oil concentrations.
The impact of long‐term care insurance system on family care: Evidence from China
Authors: Cao Siyuan , Xue Huiyuan 
Published: June 2023

AbstractAimsThe inconsistent findings on the impact of the long‐term care insurance (LTCI) system on family care require us to extend our study horizon to more countries with LTCI system designs or market practices. China has explored the LTCI system through pilot programs, which provide a quasi‐natural experimental environment. This paper aims to examine how the LTCI system affects family care in China.MethodsWe primarily employ the time‐varying difference‐in‐differences method to perform regression analyses based on the panel data from the China Health and Retirement Longitudinal Study.ResultsWe discover a 7.2% rise in family care under the LTCI system. Specifically, the LTCI system is more likely to promote family care as the relatively primary care for disabled women, disabled people aged 60–74, and those who cannot fully take care of themselves. In addition, the formal care support policy of LTCI will crowd in both formal care and family care, and the crowding‐in‐effect on formal care may even obscure the crowding‐in‐effect on family care. The family care support policy of LTCI may encourage the policy‐covered groups to take family care as their relatively primary care. It may also lengthen family care for those groups.ConclusionsThe LTCI system has a crowding‐in effect on family care. It can increase family care through cash payments or linking formal and informal care resources by providing formal community and home care.
A highly versatile biopolymer‐based platform for the maturation of human pluripotent stem cell‐derived cardiomyocytes enables functional analysis in vitro and 3D printing of heart patches
Authors: Fischer B. , Gwinner F. , Gepp M. M. , Schulz A. , Danz K. , Dehne A. , Katsen‐Globa A. , Neubauer J. C. , Gentile L. , Zimmermann H. 
Published: June 2023

AbstractHuman pluripotent stem cell‐derived cardiomyocytes (hPSC‐CMs) represent a valuable tool for in vitro modeling of the cardiac niche and possess great potential in tissue engineering applications. However, conventional polystyrene‐based cell culture substrates have adverse effects on cardiomyocytes in vitro due to the stress applied by a stiff substrate on contractile cells. Ultra‐high viscosity alginates offer a unique versatility as tunable substrates for cardiac cell cultures due to their biocompatibility, flexible biofunctionalization, and stability. In this work, we analyzed the effect of alginate substrates on hPSC‐CM maturity and functionality. Alginate substrates in high‐throughput compatible culture formats fostered a more mature gene expression and enabled the simultaneous assessment of chronotropic and inotropic effects upon beta‐adrenergic stimulation. Furthermore, we produced 3D‐printed alginate scaffolds with differing mechanical properties and plated hPSC‐CMs on the surface of these to create Heart Patches for tissue engineering applications. These exhibited synchronous macro‐contractions in concert with more mature gene expression patterns and extensive intracellular alignment of sarcomeric structures. In conclusion, the combination of biofunctionalized alginates and human cardiomyocytes represents a valuable tool for both in vitro modeling and regenerative medicine, due to its beneficial effects on cardiomyocyte physiology, the possibility to analyze cardiac contractility, and its applicability as Heart Patches.
Cellular composition modifies the biological properties and stability of platelet rich plasma membranes for tissue engineering
Authors: Anitua Eduardo , Zalduendo Mar , Troya María , Tierno Roberto , Alkhraisat Mohammad H. 
Published: June 2023

AbstractScaffolds should provide structural support for tissue regeneration, allowing their gradual biodegradation and interacting with cells and bioactive molecules to promote remodeling. Thus, the scaffold's intrinsic properties affect cellular processes involved in tissue regeneration, including migration, proliferation, differentiation, and protein synthesis. In this sense, due to its biological effect and clinical potential, Platelet Rich Plasma (PRP) fibrin could be considered a successful scaffold. Given the high variability in commercial PRPs formulations, this research focused on assessing the influence of cellular composition on fibrin membrane stability and remodeling cell activity. The stability and biological effect were evaluated at different time points via D‐dimer, type I collagen and elastase quantification in culture media conditioned by Plasma Rich in Growth Factors – Fraction 1 (PRGF‐F1), Plasma Rich in Growth Factors – Whole Plasma (PRGF‐WP) and Leukocyte‐rich Platelet Rich Plasma (L‐PRP) membranes, and by gingival fibroblast cells seeded on them, respectively. Ultrastructure of PRP membranes was also evaluated. Histological analyses were performed after 5 and 18 days. Additionally, the effect of fibrin membranes on cell proliferation was determined. According to the results, L‐PRP fibrin membranes degradation was complete at the end of the study, while PRGF membranes remained practically unchanged. Considering fibroblast behavior, PRGF membranes, in contrast to L‐PRP ones, promoted extracellular matrix biosynthesis at the same time as fibrinolysis and enhanced cell proliferation. In conclusion, leukocytes in PRP fibrin membranes drastically reduce scaffold stability and induce behavioral changes in fibroblasts by reducing their proliferation rate and remodeling ability.
Development of a novel vinylene‐linked covalent organic framework decorated with multi‐walled carbon nanotubes for electrochemical detection of tryptophan, uric acid, and 8‐hydroxy‐2′‐deoxyguanosine
Authors: Chung Chao‐Lin , Shih Tsung‐Ting , Lee Hui‐Ling 
Published: June 2023

AbstractBackgroundIn this study, a vinylene‐linked covalent organic framework (V‐COF‐1) was integrated with multi‐walled carbon nanotubes (MWCNTs) to create a modified electrode (V‐COF‐1/MWCNTs/SPCE) for electrochemical sensing applications.ObjectiveThis research focus to investigate the application of the modified electrode for the simultaneous determination of tryptophan (Trp), uric acid (UA), and 8‐hydroxy‐2'‐deoxyguanosine (8‐OHdG) in human urine samples and the mechanism between V‐COF‐1 and the analytes (Trp, UA, and 8‐OHdG) by studying tryptophan metabolites, dopamine, and L‐3,4‐dihydroxyphenylalanine (L‐DOPA).MethodsThe electrochemical performance of V‐COF‐1/MWCNTs/SPCE was evaluated using differential pulse voltammetry (DPV) and use cyclic voltammetry (CV) to detect the modified electrode to explore its electrochemical properties.ResultsUnder optimized conditions, V‐COF‐1/MWCNTs/SPCE exhibited efficient electron transfer for Trp, UA, and 8‐OHdG, with low detection limits (S/N = 3) of 0.03 μM, 0.06 μM, and 0.006 μM, respectively. The modified electrode demonstrated high selectivity for the target analytes in the presence of interfering species, as well as excellent repeatability and stability. The recoveries of Trp, UA, and 8‐OHdG in human urine samples ranged from 90.6% to 104.8%, which shows that this method has been successfully applied for the simultaneous detection of Trp, UA and 8‐OHdG in biological sample analysis.ConclusionsThe integration of V‐COF‐1 and MWCNTs onto the modified electrode showed promising results for the simultaneous detection of Trp, UA, and 8‐OHdG. The developed sensor exhibited excellent sensitivity, selectivity, repeatability, and stability. These findings contribute to the advancement of electrochemical sensor technology for biomedical applications and highlight the potential of V‐COF‐1/MWCNTs/SPCE in analytical chemistry.
Supporting Prospective Pregnancy Trials via Modeling and Simulation: Lessons From the Past and Recommendations for the Future
Authors: Cheung S. Y. Amy , Barrett Jeffrey S. 
Published: June 2023

AbstractDespite the increasing awareness and guidance to support drug research and development in the pregnant population, there is still a high unmet medical need and off‐label use in the pregnant population for mainstream, acute, chronic, rare disease, and vaccination/prophylactic use. There are many obstacles to enrolling the pregnant population in a study, ranging from ethical considerations, the complexity of the pregnancy stages, postpartum, fetus–mother interaction, and drug transfer to breast milk during lactation and impacts on neonates. This review will outline the common challenges of incorporating physiological differences in the pregnant population and historical but noninformative practice in a past clinical trial in pregnant women that led to labeling difficulties. The recommendations of different modeling approaches, such as a population pharmacokinetic model, physiologically based pharmacokinetic modeling, model‐based meta‐analysis, and quantitative system pharmacology modeling, are presented with some examples. Finally, we outline the gaps in the medical need for the pregnant population by classifying various types of diseases and some considerations that exist to support the use of medicines in this area. Ideas on the potential framework to support clinical trials and collaboration examples are also presented that could also accelerate understanding of drug research and medicine/prophylactics/vaccines in the pregnant population.
Erythema nodosum following COVID‐19 vaccination: A case report and literature review
Authors: Watanabe Chisato , Hayashi Shujiro , Mori Hitomi , Igawa Ken 
Published: June 2023

AbstractThere have been a few reported cases of erythema nodosum (EN) after coronavirus disease 2019 (COVID‐19) vaccination. A 48‐year‐old healthy woman was referred to our department with multiple erythematous nodules of varying sizes on her extremities 10 days after receiving the third COVID‐19 vaccine dose. The clinical and pathological findings of the patients were consistent with EN. We reviewed 18 EN cases post‐COVID‐19 vaccination. Considering the low number of reported EN cases, a causal link between EN and COVID‐19 vaccination is still under debate.
Aedes albopictus microbiome derives from environmental sources and partitions across distinct host tissues
Authors: Seabourn Priscilla S. , Weber Danya E. , Spafford Helen , Medeiros Matthew C. I. 
Published: June 2023

AbstractThe mosquito microbiome consists of a consortium of interacting microorganisms that reside on and within culicid hosts. Mosquitoes acquire most of their microbial diversity from the environment over their life cycle. Once present within the mosquito host, the microbes colonize distinct tissues, and these symbiotic relationships are maintained by immune‐related mechanisms, environmental filtering, and trait selection. The processes that govern how environmental microbes assemble across the tissues within mosquitoes remain poorly resolved. We use ecological network analyses to examine how environmental bacteria assemble to form bacteriomes among Aedes albopictus host tissues. Mosquitoes, water, soil, and plant nectar were collected from 20 sites in the Mānoa Valley, Oahu. DNA was extracted and associated bacteriomes were inventoried using Earth Microbiome Project protocols. We find that the bacteriomes of A. albopictus tissues were compositional taxonomic subsets of environmental bacteriomes and suggest that the environmental microbiome serves as a source pool that supports mosquito microbiome diversity. Within the mosquito, the microbiomes of the crop, midgut, Malpighian tubules, and ovaries differed in composition. This microbial diversity partitioned among host tissues formed two specialized modules: one in the crop and midgut, and another in the Malpighian tubules and ovaries. The specialized modules may form based on microbe niche preferences and/or selection of mosquito tissues for specific microbes that aid unique biological functions of the tissue types. A strong niche‐driven assembly of tissue‐specific microbiotas from the environmental species pool suggests that each tissue has specialized associations with microbes, which derive from host‐mediated microbe selection.
Two‐dimensional multichannel bandpass filter bank with improved fractional bandwidth and selectivity
Authors: Aidoo Michael W. , Song Kaijun , Zhou Yedi , Fan Yong 
Published: June 2023

AbstractThis letter presents a six‐channel bandpass filter bank created on substrate integrated waveguide (SIW) with improved bandwidth and frequency selectivity on the lower band. Through SIW square cavities and slant slots, a two‐dimensional multichannel bandpass filter bank with a transmission zero constructed with the SIW orthogonal resonant modes is produced. The substrate integrated square cavity approach is used to achieve the propagation of the two orthogonal‐oriented transverse electric modes, TE102 and TE201, which resonate on the same frequency and bandwidth, depending on the location of the feed ports. The bandwidth could be altered by the coupling matrix synthesis and coupling windows. Better transmission and bandpass responses are produced in each channel when the feed ports and coupling gaps are positioned and appropriately adjusted. However, a pair of slot lines are imprinted along each cavity's designated diagonal to guarantee better isolation while attaining an increased fractional bandwidth. A 3 × 3 array of six bandpass filters with a center frequency of 8.65 GHz has been built, simulated, and measured to establish the suggested technique. Low insertion loss, high return loss, high isolation, an improved fractional bandwidth, and a compact device were the proposal's advantages.
Inherited stress resiliency prevents the development of metabolic alterations in diet‐induced obese mice
Authors: Ben‐Shachar Michaella , Daniel Tehila , Wollman Ayala , Govindaraj Sharmila , Aviel‐Ronen Sarit , Pinhasov Albert , Rosenzweig Tovit 
Published: June 2023

AbstractObjectiveChronic stress promotes obesity and metabolic comorbidities. The ability of individuals to cope with stress may serve as an important parameter in the development of obesity‐related metabolic outcomes. The aim of this study was to clarify whether differences in stress response affect metabolic health under obesity.MethodsThe study was performed in a selectively bred mouse model of social dominance (Dom) and submissiveness (Sub), which exhibit stress resilience or vulnerability, respectively. Mice were given a high‐fat diet (HFD) or standard diet, followed by physiological, histological, and molecular analyses.ResultsThe HFD caused hyperleptinemia, glucose intolerance, insulin resistance, steatosis of the liver and pancreas, and brown adipose tissue whitening in Sub mice, whereas Dom mice were protected from these consequences of the HFD. The HFD increased circulating levels of interleukin (IL)‐1β and induced the expression of proinflammatory genes in the liver and in epididymal white adipose tissue of Sub mice, with no changes in Dom mice. The Cox2 inhibitor celecoxib (15 mg/kg/d) reduced serum IL‐1β, improved glucose tolerance and insulin sensitivity, and prevented hepatic and brown adipose tissue whitening in HFD‐fed Sub mice.ConclusionsThe extent of stress resiliency is associated with inflammation and contributes to population heterogeneity in the development of healthy or unhealthy obesity.image
Evaluation of structural and functional properties of citrus pectin film enriched with green tea extract
Authors: Wei Huan , Pascall Melvin A. 
Published: June 2023

AbstractThe effects of green tea extract (GTE) concentrations (0%, 1%, 2%, 3%, 4%, and 5%) on the thicknesses, mechanical strengths, storage/loss moduli, glass transition temperatures (Tg), chemical structures, morphologies, antioxidant activities, moisture contents, and gas permeabilities of low methoxyl pectin films were investigated in this study. A decrease in the percent elongation and moisture contents accompanied by an increase in the tensile strengths, Tg and elastic and storage moduli were observed with increasing, but low concentrations of GTE in the pectin films. This rate of change slowed at higher GTE concentrations. The thicknesses of the pectin films were not significantly affected by the concentrations of GTE. The FTIR results showed increasing ester and hydrogen bonding formations with increasing concentrations of GTE from 0% to 2% in the pectin. When the GTE concentration increased from 2% to 5%, there was an increase in ester, but a decrease in the H‐bonding. The x‐ray diffraction results showed that the crystallinity of the films increased as a result of increasing GTE contents. The results of the antioxidant activities and oxygen and water vapor barrier properties showed an enhancement due to the higher concentrations of GTE. Therefore, GTE can be incorporated into low methoxyl pectin in order to make films with desired mechanical strength, gas barrier, and antioxidant properties.
An energy‐based strategy to predict the thickness and content of randomly oriented nanolayers aggregates/agglomerates in thermoplastic polymer nanocomposites: Experimental and analytical approaches
Authors: Sharifzadeh Esmail , Azimi Neda , Zamanian‐Fard Alireza , Mohammadi Reza 
Published: June 2023

AbstractIn this study, the formation of clay nanolayer clusters inside the high‐density polyethylene (HDPE) matrix was evaluated using a specific strategy based on energy equilibrium in the melt mixing stage. Furthermore, the formed interphase region around nanoparticle domains was precisely evaluated considering the molecular structure of the matrix and linear variation of the physical/mechanical characteristics. The obtained results were used in a developed analytical model to predict the tensile modulus of the nanocomposite system containing randomly oriented nanoparticle domains. The domains consisted of exfoliated, intercalated, or aggregated/agglomerated nanolayers placed in different excluded volumes as the components of an equivalent box model. Different comparative studies were performed using the obtained data from different tests, applied to the prepared HDPE/clay nanocomposite samples, and other data from the literature. It was found that the confrontation between the exerted dispersion energy, by the mixer, and interparticle cohesion energy defined the thickness and physical/mechanical characteristics of the final aggregates/agglomerates in a nanocomposite. Though, the size and content of aggregates/agglomerates increased with the content of initially added nanoparticles. Consequently, the random orientation of each nanoparticle domain and formation of the polymer/particle interphase were proved to have determinative impacts on the mechanical properties of the system.
Meta‐analysis of the immunogenicity of standard and booster SARS‐CoV‐2 vaccination in patients with chronic liver disease and post‐liver transplantation
Authors: Wang Wen‐Xin , Wang Jitao , Jia Rui , Martini Silvia , Liu Jiaye , Huang Yifei , Wang Fu‐Sheng , Qi Xiaolong , Fu Junliang 
Published: June 2023

AbstractAimsPatients with liver disease may exhibit higher infection rates and mortality rates from coronavirus disease 2019 (COVID‐19) than healthy individuals, and vaccination against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is an effective prevention strategy. This meta‐analysis aimed to assess the effectiveness and safety of SARS‐CoV‐2 vaccines in patients with chronic liver disease (CLD) and post‐liver transplantation (LT).MethodsThe PubMed, Embase, and Cochrane databases were searched. A random‐effects model meta‐analysis was used to determine the seropositivity rates of SARS‐CoV‐2 antibodies, odds ratio (OR) compared with healthy controls (HC), risk ratio (RR) between the booster and standard vaccination regimen, and the rate of adverse reactions (ADR).ResultsIn the standard vaccination regimen analysis, 17 controlled articles were included for effectiveness analysis, and six articles for ADR analysis. The pooled seropositivity rates of SARS‐CoV‐2 antibodies in patients with CLD and post‐LT were 93.3% (95% confidence interval [CI]: 89.0%–97.6%) and 69.1% (95% CI: 63.0%–75.3%), respectively. Both rates were lower than those in HC (p < 0.001). The differences remained significant after sorting by detection interval, vaccine type, antibody type, or CLD type. LT recipients showed much lower seropositivity rates of antibodies than patients with CLD (69.1% vs. 93.3%) or HC (OR: 0.055). The pooled total ADR rate of patients was 24.0% (95% CI: 16.2%–31.8%). In the booster vaccination regimen analysis, 11 prospective studies were enrolled, and the seropositivity rates of antibodies after the booster dose were increased by 27% compared with those of the standard vaccination regimen (RR: 1.27, 95% CI: 1.15–1.41, p < 0.001).ConclusionPatients with CLD and post‐LT can gain protection against COVID‐19 from standard vaccines, demonstrating a potentially weaker immunogenic response than HC. Booster vaccines can compensate for this deficiency. Therefore, patients with CLD and post‐LT should be prioritized for receiving the COVID‐19 booster vaccine.