bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signaling through the dystrophin glycoprotein complex affects the stress-dependent transcriptome in Drosophila
Authors: Carney Travis D. , Hebalkar Rucha Y. , Edeleva Evgeniia , Çiçek Ibrahim Ömer , Shcherbata Halyna R. 
Published: January 2023

ABSTRACT
Deficiencies in the human dystrophin glycoprotein complex (DGC), which links the extracellular matrix with the intracellular cytoskeleton, cause muscular dystrophies, a group of incurable disorders associated with heterogeneous muscle, brain and eye abnormalities. Stresses such as nutrient deprivation and aging cause muscle wasting, which can be exacerbated by reduced levels of the DGC in membranes, the integrity of which is vital for muscle health and function. Moreover, the DGC operates in multiple signaling pathways, demonstrating an important function in gene expression regulation. To advance disease diagnostics and treatment strategies, we strive to understand the genetic pathways that are perturbed by DGC mutations. Here, we utilized a Drosophila model to investigate the transcriptomic changes in mutants of four DGC components under temperature and metabolic stress. We identified DGC-dependent genes, stress-dependent genes and genes dependent on the DGC for a proper stress response, confirming a novel function of the DGC in stress-response signaling. This perspective yields new insights into the etiology of muscular dystrophy symptoms, possible treatment directions and a better understanding of DGC signaling and regulation under normal and stress conditions.
Meeting report – Desmosome dysfunction and disease: Alpine desmosome disease meeting
Authors: Spindler Volker , Gerull Brenda , Green Kathleen J. , Kowalczyk Andrew P. , Leube Rudolf , Marian Ali J. , Milting Hendrik , Müller Eliane J. , Niessen Carien , Payne Aimee S. , Schlegel Nicolas , Schmidt Enno , Strnad Pavel , Tikkanen Ritva , Vielmuth Franziska , Waschke Jens 
Published: January 2023

ABSTRACT
Desmosome diseases are caused by dysfunction of desmosomes, which anchor intermediate filaments (IFs) at sites of cell–cell adhesion. For many decades, the focus of attention has been on the role of actin filament-associated adherens junctions in development and disease, especially cancer. However, interference with the function of desmosomes, their molecular constituents or their attachments to IFs has now emerged as a major contributor to a variety of diseases affecting different tissues and organs including skin, heart and the digestive tract. The first Alpine desmosome disease meeting (ADDM) held in Grainau, Germany, in October 2022 brought together international researchers from the basic sciences with clinical experts from diverse fields to share and discuss their ideas and concepts on desmosome function and dysfunction in the different cell types involved in desmosome diseases. Besides the prototypic desmosomal diseases pemphigus and arrhythmogenic cardiomyopathy, the role of desmosome dysfunction in inflammatory bowel diseases and eosinophilic esophagitis was discussed.
Fertility in Mice
Authors: Parkes A. S. 
Published: January 2023

ABSTRACT
The average size of 267 litters of albino mice totalling 1795 animals was 6.72; in addition, the distribution of fertility between the two cornua of the uterus was found to be very even. Seasonal variation in fertility was found to be slightly in favour of the summer months. Without artificially maintaining an optimum temperature, the mice were found not to breed in the months November to February. Of consecutive pregnancies the second was found to produce the largest number of young, while concurrent lactation was found to decrease the size of litter, as also, to a lesser degree, did polygyny. The interpretation of these results is attempted in the light of the two factors which govern the size of litter at birth, i.e. the number of ova ovulated and the amount of prenatal mortality.
Efficacy of magnetic resonance imaging texture features of the lateral pterygoid muscle in distinguishing rheumatoid arthritis and osteoarthritis of the temporomandibular joint
Authors: Muraoka Hirotaka , Kaneda Takashi , Hirahara Naohisa , Ito Kotaro , Okada Shunya , Kondo Takumi 
Published: January 2023

Objectives: The aim of this study was to assess whether magnetic resonance imaging (MRI) texture features of the lateral pterygoid muscle can distinguish between rheumatoid arthritis (RA) and osteoarthritis (OA) of the temporomandibular joint (TMJ). Methods: The authors extracted 279 texture features from 36 patients with RA and OA from the region of interest set for the lateral pterygoid muscle on short tau inversion recovery (STIR) images using MaZda Ver.3.3. A total of 10 texture features were selected using Fisher’s coefficients, as well as probability of error and average correlation coefficients. Data observed to have a non-normal distribution using the Kolmogorov–Smirnov test were compared using the Mann–Whitney U-test. Receiver operating characteristic (ROC) curves were used to assess the ability of the 10 texture features to distinguish RA and OA of the TMJ. Results: A total of 10 features (5 Correlation, 3 Run Length Nonuniformity, 1 Sigma, and 1 Teta) were selected from 279 texture features. These texture features revealed significant differences between the RA and OA groups (p < 0.01). The sensitivity, specificity, accuracy, and area under the ROC curve of the texture features for distinguishing RA from OA were 0.78–0.94, 0.89–1.0, 0.86–0.92, and 0.89–0.95, respectively. Conclusion: MRI texture analysis of the lateral pterygoid muscle may be useful for distinguishing between RA and OA of the TMJ.
Current trends in the development of pedagogical systems of ukraine in the conditions of digitalization of society
Authors: Potapchuk Olha 
Published: January 2023

The purpose of the work is to analyse the current trends in the development of domestic pedagogical systems in the context of digitalization of society.
The research methodology consists in the use of methods of scientific analysis, pedagogical positions on the issues of digitalization of education, implementation of systemic and integrative approaches.
The scientific novelty consists in highlighting the main trends of the prospective pedagogical system. The main trends include: humanization and humanitarianization; national orientation; openness; science; continuity; digitalization. An analysis of each trend is presented and their role in the development of education is determined. It has been established that the digitalization of education creates prerequisites for the wide implementation of psychological and pedagogical developments in pedagogical practice, which allow to intensify the educational process, implement learning ideas, including within the framework of new models.
Conclusions. Summarizing the analysis of the main trends and features of pedagogical systems at the current stage, it is very important to take into account the orientation of global educational processes. This is primarily an orientation to the individual, development of creative potential; openness of education as a condition of professional flexibility, mobility of a specialist, his ability to self-education and professional self-development; the need for interconnection and coordination of various stages of professional training, retraining and advanced training. Therefore, in order to modernize the pedagogical system of Ukraine, the national traditions and prospects of the country should be determined, taking into account the current trends in the development of digital technologies and the experience of foreign education systems.
Non-medical prescribing in primary care in the UK: an overview of the current literature
Authors: Armstrong Amanda 
Published: January 2023

Background Non-medical prescribers are perceived as a complement to busy general practice in primary care. Aim To conduct an overview of the literature available on the role and impact of non-medical prescribers on primary care patients. Method A search was conducted using multiple databases to find articles published between January 2015-January 2021. Inclusion criteria: non-medical prescribers in primary care in the UK, written in the English language. Exclusion criteria: research conducted in secondary care or outside the UK. Findings 285 studies were identified; 15 were eligible for critical appraisal. The key themes were: non-medical prescriber's positive perceptions were autonomy, job satisfaction and colleague support. The negative perceptions included risk, lack of continuous professional development and organisational support. Conclusion By reviewing the perceptions of non-medical prescribing in primary care, organisations can ensure when employing new non-medical prescribers that adequate continued professional development and support is in place. Thereby reducing the concerns of non-medical prescribers about the ligation risk of prescribing.
Characterization of Water-Soluble Ions in PM2.5 in Chongqing, a Megacity in Eastern Sichuan Basin, China
Authors: Song Tianli , Cao Xuyao , Wang Huanbo , Qiu Yang , Chen Yang , Tian Mi , Yu Jianyan , Zhai Chongzhi , Yang Fumo 
Published: January 2023

Samples of PM2.5 were collected at three urban sites and one rural site simultaneously in Chongqing, the only megacity in eastern Sichuan Basin, Southwest China, from October 15 to November 13, 2015. Water-soluble ions (WSIs, i.e., F–, Cl–, NO3–, SO42–, K+, Na+, NH4+, Mg2+ and Ca2+) in PM2.5 were measured to investigate their characteristics and formation pathways. The average concentrations of PM2.5 at the urban sites were 55.5–59.0 µg m–3, which was 62.8–73.0% higher than that at rural site. SO42–, NO3–, NH4+ were the dominant ions, contributing to more than 90% of total WSIs. The coefficients of divergence for SO42– between the urban and rural sites were 0.15–0.17, indicating its relatively uniform distribution across Chongqing. Analysis of the formation mechanisms of SO42– and NO3– in PM2.5 suggested that the heterogeneous reaction was responsible for the high concentrations of sulfate among the four sites, whereas nitrate was formed mainly through homogeneous reactions at the urban sites. Furthermore, the results of trajectory clustering showed that the air pollution were mainly from local sources within the basin. Our findings on PM2.5 composition in Chongqing help to advance the knowledge on PM2.5 pollution in Chinese megacities, and to provide more evidence for further pollution mitigation.
Multidisciplinary Development Issues of Hematopoietic Stem Cell Transplantation Program in Ukraine: Role of Auxiliary Cryopreservation Technologies
Authors: Kalynychenko Tetiana O. 
Published: January 2023

Hematopoietic stem cell transplantation (HSCT) is a life-saving medical technology for many serious diseases. Active international exchange of transplant material is ensured through productive cooperation of world international donation, transplantation, cell therapy organizations, along with their associations. Analysis of the experience of many countries has allowed the development of key recommendations from the Worldwide Network for Blood and Marrow Transplantation for establishing HSCT programs. According to them, to make the most effective use of the capabilities of this medical technology, the creation of new transplant programs requires both sufficient investment and the presence of specialized professional teams for multidisciplinary support of the entire process.
This article discusses prospects for the development of the national transplant program in Ukraine. In particular, the role of Ukrainian national scientific and practical traditions detailed in the creation of cellular processing technologies and cryopreservation as part of the team support providing components of transplantation medical technology. It is looked forward that the development of the HCST program in Ukraine will take place through continuous improvement in order to meet the criteria of the highest quality and safety. Its serious basis is the solid scientific traditions, historical and modern experience of many directions that provide the field.
Multidisciplinary or Monotherapy? Effects of Multidisciplinary Approach in Sotos Syndrome
Authors: Bingöl Rahime Hülya , Turna Fatma , Gagachovska Biljana , Kocabasoglu Nese 
Published: January 2023

Objectives: Paper presents impact of multidisciplinary approach in treatment of patients with Sotos Syndrome. Design: 36 months old female patient with Sotos syndrome was presented to us three years ago with an atypical development of communication and social interaction, inattentivity, hyperactivity and overall difficulty to manage her. She met diagnostic criteria for atipic childhood autism and specific training for autism was applied. At the age of five years and nine months Stanford Binett test was applied with a result of learning disability and the patient started receiving specific training. Results: Development was shown very quickly, she learned reading and writing, inattentivity and hyperactivity disappeared. Conclusions: Sotos syndrome is syndrome with multiple anomaly and common developmental and behavioral problems. Patients have a high risk of developing ADHD, temper tantrums and present autistic features. Academic difficulties and learning disabilities occur in most of them. Specific cognitive deficit may be present while levels of general intelligence stays unimpaired.
Deep-sequencing of viral genomes from a large and diverse cohort of treatment-naive HIV-infected persons shows associations between intrahost genetic diversity and viral load
Authors: Gabrielaite Migle , Bennedbæk Marc , Rasmussen Malthe Sebro , Kan Virginia , Furrer Hansjakob , Flisiak Robert , Losso Marcelo , Lundgren Jens D. ,   INSIGHT START Study Group, Marvig Rasmus L. 
Published: January 2023

Background
Infection with human immunodeficiency virus type 1 (HIV) typically results from transmission of a small and genetically uniform viral population. Following transmission, the virus population becomes more diverse because of recombination and acquired mutations through genetic drift and selection. Viral intrahost genetic diversity remains a major obstacle to the cure of HIV; however, the association between intrahost diversity and disease progression markers has not been investigated in large and diverse cohorts for which the majority of the genome has been deep-sequenced. Viral load (VL) is a key progression marker and understanding of its relationship to viral intrahost genetic diversity could help design future strategies for HIV monitoring and treatment.

Methods
We analysed deep-sequenced viral genomes from 2,650 treatment-naive HIV-infected persons to measure the intrahost genetic diversity of 2,447 genomic codon positions as calculated by Shannon entropy. We tested for associations between VL and amino acid (AA) entropy accounting for sex, age, race, duration of infection, and HIV population structure.

Results
We confirmed that the intrahost genetic diversity is highest in the env gene. Furthermore, we showed that mean Shannon entropy is significantly associated with VL, especially in infections of >24 months duration. We identified 16 significant associations between VL (p-value<2.0x10-5) and Shannon entropy at AA positions which in our association analysis explained 13% of the variance in VL. Finally, equivalent analysis based on variation in HIV consensus sequences explained only 2% of VL variance.

Conclusions
Our results elucidate that viral intrahost genetic diversity is associated with VL and could be used as a better disease progression marker than HIV consensus sequence variants, especially in infections of longer duration. We emphasize that viral intrahost diversity should be considered when studying viral genomes and infection outcomes.

Trial registration
Samples included in this study were derived from participants who consented in the clinical trial, START (NCT00867048) (23), run by the International Network for Strategic Initiatives in Global HIV Trials (INSIGHT). All the participant sites are listed here: http://www.insight-trials.org/start/my_phpscript/participating.php?by=site
Effect of adipose derived stromal vascular fraction on leprosy neuropathy: A Preliminary report
Authors: Sirait Sondang P. , Bramono Kusmarinah , Menaldi Sri Linuwih , Pawitan Jeanne Adiwinata , Indriatmi Wresti , Aninditha Tiara 
Published: January 2023

Background
Adipose derived stromal vascular fraction (SVF) contains a heterogeneous population of mononuclear cells, progenitor cells and about 1–10% are mesenchymal stromal cells. These cells are an ideal candidate for regenerative medicine for peripheral neuropathy. Leprosy is a disabling disorder with neuropathy, usually with consequences of permanent disability of the extremities. We conducted a preliminary study to evaluate the cell yield, its characteristics and clinical outcomes after SVF injections in four leprosy patients.

Methods
Four post leprosy patients were recruited and evaluated for sensory testing (warm detection, cold detection, vibration, pain and sensation) on the ulnar area of the hand. Liposuction was done and adipose tissue was processed into SVF with a closed system and injected to the ulnar area of the hand at the dorsal and palmar side. Evaluation of sensory testing was done after 3 days, 1 week, 1 month and 3 months following SVF injection. SVF was also characterized using flow cytometry, cell counting, sterility and presence of mycobacteria.

Results
The results showed that leprosy patients had a low count of mesenchymal cells and a high amount of CD34/CD45 positive cells. One patient was positive for mycobacteria from his adipose tissue and SVF. Sensory examination after SVF injection showed an improvement in temperature and pain sensation in the palmar and superficial branch. Meanwhile, touch sensation improved on the dorsal branch, and there was no improvement for vibration in all patients.

Conclusions
The results showed that SVF had a potential to improve sensory loss in leprosy patients.
Longitudinal proteomic profiling of the inflammatory response in dengue patients
Authors: Garishah Fadel Muhammad , Boahen Collins K. , Vadaq Nadira , Pramudo Setyo G. , Tunjungputri Rahajeng N. , Riswari Silvita Fitri , van Rij Ronald P. , Alisjahbana Bachti , Gasem Muhammad Hussein , van der Ven André J. A. M. , de Mast Quirijn 
Published: January 2023

Background
The immunopathogenesis of dengue virus (DENV) infection remains incompletely understood. To increase our understanding of inflammatory response in non-severe dengue, we assessed longitudinal changes in the inflammatory proteome in patients with an acute DENV infection.

Methods
Using a multiplex proximity extension assay (PEA), we measured relative levels of 368 inflammatory markers in plasma samples from hospitalized patients with non-severe DENV infection in the acute (n = 43) and convalescence (n = 35) phase of the infection and samples of healthy controls (n = 10).

Results
We identified 203 upregulated and 39 downregulated proteins in acute versus convalescent plasma samples. The upregulated proteins had a strong representation of interferon (IFN) and IFN-inducible effector proteins, cytokines (e.g. IL-10, IL-33) and cytokine receptors, chemokines, pro-apoptotic proteins (e.g. granzymes) and endothelial markers. A number of differentially expressed proteins (DEPs) have not been reported in previous studies. Functional network analysis highlighted a central role for IFNγ, IL-10, IL-33 and chemokines. We identified different novel associations between inflammatory proteins and circulating concentrations of the endothelial glycocalyx disruption surrogate marker syndecan-1.
Conclusion: This unbiased proteome analysis provides a comprehensive insight in the inflammatory response in DENV infection and its association with glycocalyx disruption.
Mapping hematologists’ HIV testing behavior among lymphoma patients–A mixed-methods study
Authors: Bogers Saskia , Zimmermann Hanne , Ndong Amie , Davidovich Udi , Kersten Marie José , Reiss Peter , Schim van der Loeff Maarten , Geerlings Suzanne ,   on behalf of the HIV Transmission Elimination AMsterdam (H-TEAM) Consortium
Published: January 2023

Background
HIV testing among patients with malignant lymphoma (PWML) is variably implemented. We evaluated HIV testing among PWML, and mapped factors influencing hematologists’ testing behavior.

Materials
We conducted a mixed-methods study assessing HIV testing among PWML, factors influencing HIV testing and opportunities for improvement in five hospitals in the region of Amsterdam, the Netherlands. The proportion of PWML tested for HIV within 3 months before or after lymphoma diagnosis and percentage positive were assessed from January 2015 through June 2020. Questionnaires on intention, behavior and psychosocial determinants for HIV testing were conducted among hematologists. Through twelve semi-structured interviews among hematologists and authors of hematology guidelines, we further explored influencing factors and opportunities for improvement.

Findings
Overall, 1,612 PWML were included for analysis, including 976 patients newly diagnosed and 636 patients who were referred or with progressive/relapsed lymphoma. Seventy percent (678/976) of patients newly diagnosed and 54% (343/636) of patients with known lymphoma were tested for HIV. Overall, 7/1,021 (0.7%) PWML tested HIV positive, exceeding the 0.1% cost-effectiveness threshold. Questionnaires were completed by 40/77 invited hematologists, and 85% reported intention to test PWML for HIV. In the interviews, hematologists reported varying HIV testing strategies, including testing all PWML or only when lymphoma treatment is required. Recommendations for improved HIV testing included guideline adaptations, providing electronic reminders and monitoring and increasing awareness.

Conclusions
Missed opportunities for HIV testing among PWML occurred and HIV test strategies varied among hematologists. Efforts to improve HIV testing among PWML should include a combination of approaches.
Recensione a Maddalena Bonelli (a cura di). Filosofe Maestre Imperatrici. Per un nuovo canone della storia della filosofia antica (2020)
Authors: Palumbo Lidia 
Published: January 2023

Recensione a Maddalena Bonelli (a cura di). (2020) Filosofe Maestre Imperatrici. Per un nuovo canone della storia della filosofia antica. Roma, Edizioni di Storia e letteratura.