bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discordance of HER2-low between primary tumors and matched distant metastases in breast cancer
Authors: Almstedt Katrin , Krauthauser Lisa , Kappenberg Franziska , Wagner Daniel-Christoph , Heimes Anne-Sophie , Battista Marco J , Anic Katharina , Krajnak Slavomir , Lebrecht Antje , Schwab Roxana , Brenner Walburgis , Weikel Wolfgang , Rahnenführer Jörg , Hengstler Jan G , Roth Wilfried , Hasenburg Annette , Stewen Kathrin , Schmidt Marcus 
Published: January 2023

Abstract
Background: Novel antibody-drug conjugates (ADCs) show activity in HER2-low advanced breast cancer. We examined differences in HER2 expression between primary tumors and distant metastases, particularly within the HER2-negative cohort (HER2-low and HER2-zero).
Patients and Methods: The retrospective study included 191 consecutive paired samples of primary breast cancer (BC) and distant metastases diagnosed between 1995 and 2019. HER2-negative samples were divided into HER2-zero (immunohistochemistry [IHC] score 0) and HER2-low (IHC score 1+ or 2+/in situ hybridization [ISH]-negative). The main objective was to analyze the discordance rate between matched primary and metastatic samples, focusing on the site of distant metastasis, molecular subtype, and de novo metastatic BC. The relationship was determined by cross-tabulation and calculation of Cohen's kappa coefficient.
Results: The final study cohort included 148 paired samples. The largest proportion in the HER2-negative cohort was HER2-low [primary tumor 61.4% (n=78), metastatic samples 73.5% (n=86)]. The discordance rate between HER2 status of primary tumors and corresponding distant metastases was 49.6% (n=63) (kappa -0.003, 95%CI -0.15 - 0.15). Development of a HER2-low phenotype occurred most frequently (n=52, 40.9%), mostly with a switch from HER2-zero to HER2-low (n=34, 26.8%). Relevant HER2 discordance rates were observed between different metastatic sites (with the exception of lung/pleural metastases) and molecular subtypes. Primary metastatic breast cancer had a significantly lower HER2 discordance rate than secondary metastatic breast cancer [30.2% (kappa 0.48, 95%CI 0.27 - 0.69) versus 50.5% (kappa 0.14, 95% CI -0.03 - 0.32)].
Conclusion: In HER2-negative BC, there is a significant discrepancy between primary tumor and distant metastases. In particular, for the HER2-low cohort, biopsy of metastases opens new therapeutic opportunities with novel ADCs such as trastuzumab-deruxtecan.
Amiodarone and lidocaine infusion for treatment of ventricular tachycardia storm in an emergency setting: Case report
Authors: Awad Antony , Baird James F 
Published: January 2023

Abstract
This report describes a case of ventricular tachycardia (VT) storm managed in the emergency department prior to transfer for automatic implantable cardioverter-defibrillator (AICD) placement. VT storm is associated with significant in-hospital and 6-month mortality, necessitating emergent stabilization and definitive secondary prevention. While ACLS and antiarrhythmic drugs (AAD) are the mainstays of emergency management early referral is indicated for AICD placement, catheter ablation, and/or sympathetic cardiac denervation. In the setting of sustained or recurrent VT (including VT storm), amiodarone and/or lidocaine can be administered not only as boluses but also as infusions. This strategy can stabilize the patient for transfer (if needed) and avoid escalation to intubation and sedation for central sympathetic blockade, which would necessitate admission to intensive care.
Experimental investigation on the solubility of 3,3,3-trifluoropropene in [hmim][TfO] and [omim][TfO] ILs from 303.15 K to 343.15 K
Authors: Jia Xiucan , Ma Long , Wang Xiaopo 
Published: January 2023

Abstract
The working pairs composed of hydrofluoroolefins (HFOs) and ionic liquids (ILs) have exhibited promising potential in the absorption refrigeration systems (ARS). In order to exploit the possibility of 3, 3, 3-trifluoropropene/ILs as the pairs used in the ARS, the experimental determination on the solubility of 3,3,3-trifluoropropene in 1-hexyl-3-methyl-imidazolium trifluoromethanesulfonate ([hmim][TfO]) and 1-octyl-3-methyl-imidazolium trifluoromethanesulfonate ([omim][TfO]) ILs was conducted. The present measurements were carried out based on the isochoric saturation method at the temperature range from 303.15 K to 343.15 K. The experimental solubility data were correlated through the non-random two liquid (NRTL) model and Krichevsky-Kasarnovsky (K-K) model, respectively. In addition, the influence of the alkyl chain length for different ILs on the solubility of R1243zf and the dissolving capacities of different HFOs in [hmim][TfO] were compared. Furthermore, the Henry’s constants and mixing thermodynamic properties (enthalpy, entropy and Gibbs Energy) of R1243zf in [hmim][TfO] and [omim][TfO] were calculated and discussed.
Effects of Different Ratios of BMP2 and VEGFA on the Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells
Authors: Li Juan , Huang Jiameng , Shang Yufeng , Yang Zhenze , Lin Jun , Wang Huiming 
Published: January 2023

Abstract
Bone morphogenetic protein-2 (BMP2) and vascular endothelial growth factor (VEGF) have been shown to synergistically promote bone formation via cell-based gene therapy. However, the optimal ratio of BMP2 to VEGFA required to enhance the beneficial effect on bone formation remains unclear. In the present study, bone narrow-derived mesenchymal stem cells (BMSCs) were adenovirally transduced to express BMP2 and VEGFA. Cell proliferation and the expressions of transduced genes BMP2 and VEGF were examined.The different proportion of BMP2 and VEGF (BMP-2:VEGFA = 1:3, 1:1, 3:1; BMP2 only; VEGFA only; control) in BMSCs were applied. After 2–3 weeks, Osteocalcin (OC) and Runt-related transcription factor 2 (Runx2), ALP activity, and ARS staining were detected. Our results showed that the high ratio of BMP2 to VEGFA enhanced bone formation and the low ratio lost the beneficial effect on the bone formation. The present study revealed that the synergistic effect of BMP2 and VEGFA on bone healing depends critically on the ratio of these two genes, providing important evidence for cell-based gene therapy and tissue engineering for bone healing.
Short-term Changes in Tear Osmolarity after Instillation of Different Osmolarity Eye Drops in Patients with Dry Eye
Authors: Kim Woojin , Woo In Ho , Eom Youngsub , Song Jong Suk 
Published: January 2023

Abstract
This study comprised 80 patients with dry eye whose tear osmolarity measurement using the TearLab® os molarity system was 300 mOsm/L or greater. Patients who had external ocular disease, glaucoma, or other concomitant ocular pathology were excluded. After being randomly divided into four groups, the participants received different kinds of SH eye drops as follows: Groups 1–3 were given one of three concentrations (0.1%, 0.15%, and 0.3%) of isotonic drops, while Group 4 received 0.18% hypotonic SH eye drops. The tear osmolarity concentrations were evaluated at baseline and again at 1-, 5-, and 10-minutes after instillation of each eye drop.
Tear osmolarity showed a significant decrease after instillation of four types of SH eye drops after up to 10 minutes compared to baseline. Patients who received hypotonic SH eye drops showed an enhanced decrease in tear osmolarity compared with the isotonic SH eye drops after 1 minute (p < 0.001) and 5 minutes (p = 0.006), but the difference was not significant at 10 minutes (p = 0.836).
The enhanced immediate effect of hypotonic SH eye drops at lowering tear osmolarity in patients with dry eye seems to be limited unless these drops were used frequently.
Effects of Irrigation Water Salinity Levels on Pepper Plant (Capsicum Annuum) Yield and Quality Properties and Soil Characteristics
Authors: Maow Bashir A. , TEMİZEL Kadir Ersin 
Published: January 2023

Abstract
Plant tissues are 90% water. More efficient agricultural production techniques are needed to meet worldwide demand for water and food safety. In dry and semiarid locations, salty water is used for crop irrigation due to water constraint. Salinity, a major abiotic factor limiting agricultural productivity globally, affects osmotic adjustment and solute intake, causing many negative changes in morphological and physiological properties like photosynthetic rate and plasma membrane permeability of many economic plants. In this study, three different levels of EC were used and their effect on growth, yield, plant water consumption, chlorophyll content, soil and leaf ions, and soil ECe was determined. The resulting results are summarized as follows. The study found the increase in EC levels decreased the yield, biomass of the plants, leaf and root weights, leaves number, chlorophyll content of the leaves, and plant water consumption. EC treatments also increased the ion accumulation of leaves and soils.
Physiotherapy training and education prior to elective caesarean section and its impact on post-natal quality of life: A secondary analysis of a randomized controlled trial
Authors: Weerasinghe Kalani , Rishard Mohamed , Brabaharan Subhani , Walpita Yasasvi 
Published: January 2023

Abstract
Background
Caesarean section (CS) is associated with numerous complications that lead to the delayed return to functional activities that have a negative influence on the post-natal quality of life (QOL). It is evident that providing regular evidence-based physiotherapy training and education prior to elective CS helps to enhance the post-natal QOL by improving physical, mental, social, and general well-being. The purpose of this study was to examine the effectiveness of physiotherapy training and education prior to elective CS on post-natal QOL.
Methods
This single-blind parallel randomized controlled study was carried out at De Soysa Hospital for Women (DSHW), Colombo. The study enrolled 54 women who were scheduled to undergo elective CS. The intervention group (n = 27) of women received physiotherapy training and education, while the control group (n = 27) received standard nursing care. In addition to the primary outcome measures, post-natal QOL was measured. The results were examined using descriptive statistics and the independent samples t-test in IBM SPSS 20.
Results
The intervention group showed a higher post-natal QOL for the domains of physical function, role limitation due to physical health, energy/fatigue, and pain than the control group (p < 0.05).
Conclusion
Physiotherapy training and education prior to elective CS play a pivotal role in improving the physical health-related domains of QOL following CS.
Trial Registration:
SLCTR/2019/029-APPL/2019/028; Registered on 6th of September 2019
Study adaptive backstepping control for My quadrotor
Authors: Duc Viet Nguyen , Ngoc Giang Le , Haiquan Zhao , Thi Ngoat Tran 
Published: January 2023

Abstract
Flight control for a quadrotor is a complicated task because of the complexity of the flight trajectory program and the uncertain changes in aerodynamic parameters, which make the system more complicated and nonlinear. Flight path angle tracking control consists of varied channels, such as a pitch controller, a roll controller, and a yaw controller. They are usually designed using traditional linear control methods, but this is not always satisfying, hence the more effective approach to controller design is backstepping. The backstepping control laws not only give the controllers certain gain margins but also deal with model errors in the description of aerodynamics. This paper presents a backstepping nonlinear controller design method for control systems involving quadrotors. Simulation results show that the control strategy proposed in this paper is effective and has strong robustness in the presence of disturbances and parameter uncertainty.
The impact of Federal Open Market Committee post-meeting statements on financial markets – a text-mining approach
Authors: Wójcik Piotr , Osowska Ewelina 
Published: January 2023

Abstract
This article examines the impact of Federal Open Market Committee (FOMC) statements on stock and foreign exchange markets with the use of text-mining and predictive models. Unlike in most previous studies, both linear and non-linear methods were applied. We estimate models using both the scheduled time of statement releases and the time the public first learns about the result of meetings. In addition, we also take into account additional explanatory variables that control for the current corporate managers’ and retail customers’ assessment of the economic situation. The proposed methodology is based on calculating the FOMC statements’ tone (called sentiment) and incorporate it as a potential predictor in the modeling process. For the purpose of sentiment calculation, we utilized the FinBERT pre-trained NLP model. Fourteen event windows around the event are considered. We proved that the information content of FOMC statements is an important predictor of the financial markets’ reaction directly after the event. In the case of models explaining the reaction of financial markets in the first minute after the scheduled announcement of the FOMC statement, the sentiment score was the second most important feature, after the market surprise component. We also showed that applying non-linear models resulted in better prediction of market reaction due to identified non-linearities in the relationship between the two most important predictors (surprise component and sentiment score) and returns just after the event. Last but not least, the predictive accuracy during the COVID pandemic was indeed lower than in the previous year.
C3AR1 promotes ovarian cancer progression and induces suppressive immunocyte infiltration: evidence from comprehensive bioinformatics analysis and experiments
Authors: Huang Jinfa , Zhou Lei , Deng Kaixian 
Published: January 2023

Abstract
Background
C3AR1 was reported in driving tumor immunity in multiple cancers. However, its roles in ovarian cancer remain unclear. This study aims to determine role of C3AR1 in prognosis and regulating tumor infiltrating immune cells of ovarian cancer (OC).
Material and Methods
The expression, prognosis and clinical data related to C3AR1 were collected from public databases such as The Cancer Genome Atlas (TCGA), Human Protein Atlas (HPA) and Clinical Proteomics Tumor Analysis Alliance (CPTAC), and further analyze their relationship with immune infiltration. Immunohistochemistry verified the expression of C3AR1 in ovarian cancer and control tissues. C3AR1 was forced expressed in SKOV3 cells by plasmid transfection, and verified by qRT-PCR and Western blot. Cell proliferation were evaluated by EdU assay.
Results
Bioinformatics analysis (TCGA, CPTAC) and immunohistochemical staining of clinical samples confirmed higher C3AR1 expression in ovarian cancer than that in normal tissues. High C3AR1 expression predicted adverse clinical outcome. KEGG and GO analysis showed that the biological processes of C3AR1 in ovarian cancer are mainly involved in T cell activation, cytokine and chemokine activation. C3AR1 expression was positively correlated with chemokines and their receptors in the tumor microenvironment, such as CCR1(R = 0.83), IL10RA (R = 0.92), and INFG (R = 74). In addition, increased C3AR1 expression predicted more infiltration of tumor-associated macrophages, dendritic cell and CD8 + T cell. Some important m6A regulators, such as IGF2BP2, ALKBH5, IGFBP3 and METL14, are significantly positively or negatively correlated with C3AR1. Finally, overexpression of C3AR1 significantly increased proliferation of SKOV3 cells.
Conclusion
In summary, our study suggest that C3AR1 is associated with the prognosis and immune cell infiltration of ovarian cancer, and is a promising immunotherapeutic target.
Genome-wide identification and characterization of TCP transcription factors in peanut
Authors: Li Shuang , Zhang Xiaojun , Zhang Xiaohong , Xu Yongju , Hou Rui , Zhu Xunlu 
Published: January 2023

Abstract
The plant-specific TCP transcription factors (TFs) play important roles in multiple processes of plant development and environmental stress response. However, the characteristics of TCP TFs in peanut are still unclear. In this study, we characterized the peanut TCPs including the numbers, classification, structures, and expression patterns based on the information from the available database. We identified 28, 9, and 8 TCPs in A. hypogaea cv. Tifrunner (AhTCP1-28), A. duranensis (AdTCP1-9), and A. ipaensis (AiTCP1-8), respectively. These genes can be divided into three groups based on the phylogenetic tree: PCF, CIN, and CYC/TB1. A total of 10 paralogous gene pairs in A. hypogaea cv. Tifrunner, five orthologous gene pairs between A. duranensis and A. hypogaea cv. Tifrunner and five orthologous gene pairs between A. ipaensis and A. hypogaea cv. Tifrunner, were identified respectively. Most duplicated gene pairs underwent purifying selection except AdTCP2-AhTCP15 and AiTCP8-AhTCP28 were subjected to positive selection. Hormone-related and abiotic stress response elements were abundant in several TCP gene promoters in A. hypogaea cv. Tifrunner. AhTCP12 and AhTCP25 contained four EREs (ethylene-response elements), and AhTCP15 included four TCA elements. Most of TCPs were expressed lowly in 22 tissues, however, several genes such as AhTCP25 and a homologous gene pair, AhTCP13 and AhTCP28, were expressed highly in peg tip and perianth, respectively. AhTCP duplicated gene pairs did not show significant functional differentiation. This study provided insight into the evolution and expression of AhTCPs and might assist peanut molecular breeding.
Prognostic value of left atrial strain in patients with Tetralogy of Fallot
Authors: Mutluer Ferit Onur , Bowen Daniel J. , Grootel Roderick W. J. , Kardys Isabella , Roos-Hesselink Jolien W. , Bosch Annemien E. 
Published: January 2023

Abstract
PURPOSE:
To demonstrate prognostic utility of left atrial strain(LAS) in adult patients with repaired Tetralogy of Fallot(rTOF).
METHODS:
Adults patients with rTOF were prospectively enrolled in this study between years 2011-2015. Left atrium(LA) phasic functions were assessed using 2D speckle tracking echocardiography(2D-STE). Association of LA strain(LAS) with primary(any cardiovascular event) and secondary(death, heart failure or arrhythmia) composite endpoints was assessed using Cox regression analysis.
RESULTS:
Hundred-and-twelve rTOF patients, in whom LAS was feasible and were in sinus rhythm, were included in the final analysis (age 33±10 years, 68[61%] male). Median duration of follow-up was 8.6[4.2-9.7] years in the study group. Primary composite endpoint was reached in 48 patients (mean event-free survival time: 7.2[6.6-7.9] years), and secondary composite endpoint was reached in 22 patients (mean event-free survival time: 8.7[8.1-9.2] years). LAS-r was defined as tertile groups(1st tertile<33 %,2nd tertile=33-44%,3rd tertile>44%). Decreasing tertiles of LAS-r was associated with primary and secondary composite endpoints in Kaplan-Meier analysis(p=0.02 and 0.002, respectively). In univariable Cox-regression, both decreasing LAS-r and LAS-r tertiles were associated with primary and secondary composite endpoints. Adjusted by initial repair age and NT-proBNP quartiles, increased LAS-r was associated with significantly decreased occurrence of experiencing any events(HR=0.97,CI:0.93-0.99,p=0.037]). Decreasing LAS-r was still associated with primary endpoint when adjusted by left atrium volume index(LAVImax)(HR=0.96,CI:0.03-0.99,p=0.01),left ventricle global longitudinal strain(LV-GLS)(0.95,95%CI:0.92-0.98,p=0.002) or right ventricle free wall longitudinal strain (RV-FWLS)(0.96,95%CI:0.93-0.99,p=0.04).
CONCLUSION:
Assessment of LA mechanics with the use of STE has incremental utility in determination of mortality and morbidity in rTOF, and may be implemented in clinical practice.
Research on the Effect of Mindfulness Practice on Susceptibility to Evaluative Conditioning
Authors: Ye Jinhui , Zhong Liangjing , Li Bonan , Hu Shengyuan , Wang Xiaoyan 
Published: January 2023

Abstract
Background
Susceptibility to evaluative conditioning(EC) is associated with many social cognitive and psychological biases that are key to psychological problems. For example, depression and anxiety are closely related to susceptibility to negative stimuli, and susceptibility to positive stimuli is associated with healthy psychology and well-being. Therefore, susceptibility to evaluative conditioning is closely related to mental health and cannot be ignored. As a factor that can affect the susceptibility to evaluative conditioning, mindfulness has only recently received attention. This study aims to to provide more targeted services for health clubs wishing to support well-being and mental health.
Methods
To explore the feasibility and effect of mindfulness interventions on susceptibility to evaluative conditioning, 72 subjects were included in a fitness club in Hefei. Thirty-six of the subjects participated in mindfulness training with the Mindful Attention Awareness Scale (MAAS), Positive and Negative Emotion Scale (PANES), and using the evaluative conditioning paradigm(picture-picture paradigm) for examination.
Results
The results of the correlation and comparison of differences showed susceptibility to evaluative conditions, and the difference in the experimental group was significant before and after the intervention (P ༜0.05).
Conclusions
Compared to a control group, 12 hours of yoga and mindfulness training improved mindfulness, as measured on the Mindful Attention Awareness Scale, a decrease in negative susceptibility leads to a relative increase in positive susceptibility, therefore, people can feel more positive and have better mental health.
The effect of azithromycin on sputum inflammatory markers in bronchiectasis. Analysis from the BAT randomized controlled trial
Authors: Terpstra L. C. , Altenburg J. , Doodeman H. J. , Piñeros Y. S. Sabogal , Lutter R. , Heijerman H. G. M. , Boersma W. G. 
Published: January 2023

Abstract
Background
Long term macrolide treatment has been found beneficial in bronchiectasis (BE) -pathogical bronchial dilatation- possibly due to a combined anti-bacterial and immunomodulatory effect. The exact mechanism of inflammatory response is unknown. Here, we investigated the effect of maintenance macrolide treatment on the inflammatory response in BE. In addition, we assessed the inflammatory profile in BE in relation to disease severity.
Methods
During the BAT randomized controlled trial (investigating the effect of 1 year of azithromycin (AZM) in 83 BE patients), data on BE severity, lung function and sputum microbiology was collected. For the current study, a wide range of inflammatory markers were analysed in 3- monthly sputum samples in all participants.
Results
At baseline, marked neutrophilic but also eosinophilic inflammation was present in both groups, which remained stable throughout the study and was not affected by AZM treatment. Significant upregulation of pro-inflammatory markers correlated with FEV1 < 50% (TNFα, ECP, IL-21, IL-1, p = 0.01–0.05), H. influenzae (HI) colonization (MPO, ECP, MIP-1, TNFα, IL-21, Il-8, IL-1, IL-1α, p < 0.001–0.04) and number of exacerbations (MPO, ECP, VEGF, MMP-9, p = 0.003–0.01). Surprisingly, colonization with P. aeruginosa (PA) was found to correlate with an attenuated inflammatory response compared to non-PA colonized. In placebo-treated patients, presence of an infectious exacerbation was reflected by a significant excessive increase in inflammation as compared to a non-significant upregulation in the AZM-treated patients.
Conclusion
One year of AZM treatment did not result in attenuation of the inflammatory response in BE. Increasing disease severity and the presence of an exacerbation were reflected by upregulation of pro-inflammatory markers.