bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Features of Androgen-Receptor Low, Estrogen Receptor-Negative Breast Cancers in the Carolina Breast Cancer Study
Authors: Jinna Nikita , Alsten Sarah Van , Rida Padmashree , Seewaldt Victoria , Troester Melissa 
Published: March 2023

Abstract
Purpose: Androgen receptor (AR) expression is absent in 40-90% of estrogen receptor (ER)-negative breast cancers. The prognostic value of AR in ER-negative patients and therapeutic targets for patients absent in AR remains poorly explored.
Methods: We used an RNA-based multigene classifier to identify AR-low and AR-high ER-negative participants in the Carolina Breast Cancer Study (CBCS; n=669) and The Cancer Genome Atlas (TCGA; n=237). We compared AR-defined subgroups by demographics, tumor characteristics, and established molecular signatures [PAM50 risk of recurrence (ROR), homologous recombination deficiency (HRD), and immune response].
Results: AR-low tumors were more prevalent among Black (relative frequency difference (RFD) = +7%, 95% CI = 1% to 14%) and younger (RFD = +10%, 95% CI = 4% to 16%) participants in CBCS and were associated with HER2-negativity (RFD = -35%, 95% CI = -44% to -26%), higher grade (RFD = +17%, 95% CI = 8% to 26%), and higher risk of recurrence scores (RFD = +22%, 95% CI = 16.1% to 28%), with similar results in TCGA. The AR-low subgroup was strongly associated with HRD in CBCS (RFD = +33.3%, 95% CI = 23.8% to 43.2%) and TCGA (RFD = +41.5%, 95% CI = 34.0% to 48.6%). In CBCS, AR-low tumors had high adaptive immune marker expression.
Conclusion: Multigene, RNA-based low AR expression is associated with aggressive disease characteristics as well as DNA repair defects and immune phenotypes, suggesting plausible precision therapies for AR-low, ER-negative patients.
Spatiotemporal analysis of periodontitis—the unique role of the IL-33/ST2 axis
Authors: Liu Anhao , Hayashi Mikihito , Ohsugi Yujin , Katagiri Sayaka , Akira Shizuo , Iwata Takanori , Nakashima Tomoki 
Published: March 2023

Abstract
Periodontitis, which is induced by repeated bacterial invasion and the ensuing immune reactions that follow, is the leading cause of tooth loss. However, studies on immune responses are usually based on gingival tissue, although periodontal tissue is actually comprised of four different components. Here, we have developed a novel model to analyze the pathogenesis of periodontitis with different periodontal tissue components. We have found that the inflammatory response in the peri-root tissues and the expression of interleukin (IL)-6 and nuclear factor κ-Β ligand (RANKL) by Thy-1.2– fibroblasts/stromal cells were prominent during the course of bone destruction. Furthermore, a comprehensive analysis of the initiation phase confirmed these findings while revealing a high level of expression of ST2 (also known as IL33R and encoded by Il1rl1) in the peri-root tissue. Il1rl1 and Il33 deficient mice exhibited exacerbated bone loss in the acute phase of periodontitis, demonstrating the protective role of the IL-33/ST2 axis in acute inflammation. Thus, the findings obtained with this novel model show the crucial role of the peri-root tissue and advance our understanding of the etiology of periodontitis.
Surgical management of transcervical gunshot injury presented with CSF leak and progressive left upper extremity paresis: A case report and literature review
Authors: Roohollahi Faramarz , Rabiee Shervin , Jangholi Ehsan , Shafizadeh Milad , Rostami Mohsen , Jouibari Morteza Faghih 
Published: March 2023

Abstract
Introduction: Penetrating neck trauma (PNT) due to gunshot injuries is one of the challenging conditions with the potential for both major morbidities and mortality.
Research Question: There are major concerns in approach to patients with spinal gunshot injury. Surgery indications, methods of surgery and management of CSF leak are main concerns in these patients.
Methods and Materials: An 11-years-old boy was referred to our center with a single gunshot wound to the left side of the posterior cervical region since two days ago with cerebrospinal fluid leakage and left arm weakness.
Results: The patient underwent surgery, and the pellet was removed. His left arm weakness fully recovered after the operation. No new symptoms developed during 1 year follow up.
Conclusion: Timely surgery could dramatically improve outcomes in PNT patients with mild symptoms and prevent worsening neurological defects.
Effect of Low-pressure Pneumoperitoneum on Pain and Inflammation in Laparoscopic Cholecystectomy: A Randomized Controlled Clinical Trial
Authors: Rashdan Mohammad , Daradkeh Salam , Al-Ghazawi Mutasim , Abuhmeidan Jareer Heider , Mahafthah Azmi , Odeh Ghada , Al-Qaisi Mohammad , Salameh Ikram , Halaseh Shahed , Al-Saba Lana , Ahmad Yousef B. , Al-Ghazawi Tuqa , Al-Said Mahmoud , Sha’bin Shereen , Mansour Hanan 
Published: March 2023

Abstract
Objective
We aim to assess the effect of low-pressure pneumoperitoneum on post operative pain and ten of the known inflammatory markers.
Background
The standard of care pneumoperitoneum set pressure in laparoscopic cholecystectomy is set to 12–14 mmHg, but many societies advocate to operate at the lowest pressure allowing adequate exposure of the operative field. Many trials have described the benefits of operating at a low-pressure pneumoperitoneum in terms of lower post operative pain, and better hemodynamic stability. But only few describe the effects on inflammatory markers and cytokines.
Methods
A prospective, double-blinded, randomised, controlled clinical trial, including patients who underwent elective laparoscopic cholecystectomy. Patients randomised into low-pressure (8–10 mmHg) vs. standard-pressure (12–14 mmHg) with an allocation ratio of 1:1. Perioperative variables were collected and analysed. Registered on ClinicalTrials.gov (NCT05530564/ September 7th, 2022).
Results
one hundred patients were allocated, 50 patients in each study arm. Low-pressure patients reported lower median pain score 6-hour post operatively (5 vs 6, p-value = 0.021) in comparison with standard-pressure group. Eight out of 10 inflammatory markers demonstrated better results in low-pressure group in comparison with standard-pressure, but the effect was not statistically significant. Total operative time and surgery difficulty was not significantly different between the two groups even in the hands of inexperienced surgeons.
Conclusion
low-pressure laparoscopic cholecystectomy is associated with less post operative pain and lower rise of inflammatory markers. It is feasible with comparable complications to the standard of care.
Easy preparation of wood-base membrane with fouling resistance in complex environments for efficient oil-water emulsion separation
Authors: Zhang Xichun , Teng Chao , Zhai Rui , Jiang Lulu , Wang Jie , Zhou Jiale , Wang Rongfang , Wang Hui , Wang Xuyun , Ma Xiaoyan 
Published: March 2023

Abstract
Oily wastewater poses a serious threat to the ecological environment and human health, and there is a great deal of concern about how to treat it effectively. Although there has been considerable development in recent years in the treatment of oil-water emulsions using separation membranes with special wettability, they still suffer from real problems such as complex preparation processes, material contamination, so developing an economical and environmentally friendly, high-performance membrane is a significant challenge. In this work, a wood-based membrane was easily prepared by a simple dipping process using aramid nanofiber (ANF) to modify the surface of wood. The wet ANF/wood membrane reveals higher tensile strength (1.69±0.32 MPa) than synthetic hydrogel membranes. More importantly, the membrane presents the underwater superoleophobic properties and fouling resistance under complex environmental conditions (acid, alkali, seawater, and high temperature), and effectively separate various oil-water emulsions with high separation efficiency (>99.3%) and flux (>227 L m-2 h-1). More excitingly, the membrane remains the original separation properties after 13 cycles of oil-water emulsion separation, Therefore, the inexpensive, environmentally friendly and easily prepared ANF/wood membrane is well tolerated under extreme conditions, presents excellent separation performances and provides a material basis for the treatment of actual oily wastewater.
Evidence of Autoinflammation as the Principal Mechanism of Myocardial Injury in SARS-CoV-2 PCR-Positive Autopsy Hearts
Authors: Hammond Margo Elizabeth , Christensen Erik D , Belenky Michael , Snow Gregory L , Shah Kevin , Hammond M. Elizabeth H 
Published: March 2023

Abstract
Background
Disease from Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) remains the third leading cause of death in the United States, after cancer and heart disease. Many patients infected with this virus develop cardiovascular complications including myocardial infarctions, stroke, arrhythmia, heart failure, and sudden cardiac death. Specifically, patients with SARS-CoV-2 have a high prevalence of severe myocardial injury (20–28%). The purpose of this study is to understand the primary mechanism of myocyte injury in patients infected with SARS-CoV-2.
Methods
We investigated a consecutive cohort of 84 medical examiner cases who died with PCR-positive SARS-CoV-2 (COVpos) infection prior to availability of therapy or vaccines. We compared them to a consecutive cohort of 42 age- and sex-matched controls who were PCR-negative for SARS-CoV-2 (COVneg). Formalin-fixed paraffin embedded sections of left and right ventricle were examined on each case using antibodies directed against CD42 (platelets), CD15 (myeloid cells), CD68 (monocytes), C4d, Fibrin, CD34 (stem cell antigen), CD56 (natural killer cells), and Myeloperoxidase (MPO) (neutrophils and NETs). Slides were scanned using an Aperio slide scanner and viewer and each digital slide was entirely examined at 5x,10x and 20x. Each slide was graded using a 0–3 scale where 3 indicates the marker was present in every field at 20x. We used a Welch 2-sample T-test to determine significance.
Results
We found a significant difference between COVpos and COVneg samples for all markers, all of which were significant at p < 0.001. The most prominent features were neutrophils (CD15, MPO) and MPO positive debris suggestive of NETS and were located in or around arterioles, venules, and capillaries. A similar distribution of platelets, monocytes, and C4d was seen in COVpos cases. Fibrin was found scattered in arterioles, venules, interstitial regions, and within ventricular cavities. CD34 highlighted vascular alterations of endothelial cells in some but not all cases.
Conclusion
Autoinflammation is the primary mechanism of myocyte injury observed in COVpos hearts. The significant increase in platelets, monocytes, and neutrophils and the presence of neutrophil NETs, C4d, and fibrin suggest that myocardial injury involves neutrophils, NETosis, coagulation, complement activation, and monocyte accumulation.
Utility of near infrared fluorescent cholangiography in detecting biliary structures during challenging minimally invasive cholecystectomy.
Authors: Piccolo Gaetano , Barabino Matteo , Lecchi Francesca , Formisano Giampaolo , Salaj Adelona , Piozzi Guglielmo Niccolo , Bianchi Paolo Pietro 
Published: March 2023

Abstract
Background
Surgeons can minimize the risk of bile duct injury (BDI) during challenging mini-invasive cholecystectomy through technical standardization by means of a precise anatomical landmark identification (Critical View of Safety) and advanced technology for biliary visualization. Among these systems, the adoption of magnified stereoscopic 3-dimensional view provided by robotic platforms and near infrared fluorescent cholangiography (NIRF-C) are the most promising.
Methods
In this prospective cohort study we evaluated all consecutive minimally invasive cholecystectomies (laparoscopic and robotic) performed with NIRF-C between May 2022 and January 2023 at General Surgery Unit, Department of Health Sciences, University of Milan, San Paolo Hospital (Milan, Italy). Inclusions criteria were: 1) acute cholecystitis (emergency group), 2) history of chronic cholecystitis or complicated cholelithiasis (deferred urgent group), 3) difficult cases (patients affected by cirrhosis, with scleroatrophic gallbladder or BMI > 35 kg/m2). For each group, the detection rate and visualization order of the main biliary structures were reported (cystic duct, CD; common hepatic duct, CHD; common bile duct, CBD; and CD-CHD junction).
Results
A total of 101 consecutive patients were enrolled, including 83 laparoscopic and 18 robotic cholecystectomies. All patients were stratified into three subgroups: a) emergency group (n = 33, 32.7%), b) deferred urgent group (n = 46, 45.5%), c) difficult group (n = 22, 21.8%). Visualization of at least one biliary structure was possible in 94.1% of cases (95/101). Interestingly, all four main structures were detected in 43.6% of cases (44/101). The CD was the structure identified most frequently, being recognized in 91/101 patients (90.1%), followed by CBD (83.2%), CHD (62.4%), and CD-CHD junction (52.5%). In the subset of patients that underwent emergency surgery for AC, the CD-CHD confluence was identified in only 45.5% of cases. However, early and precise identification of CBD (75.8%) and CD (87.9%) allowed safe isolation, clipping and transection of the cystic duct. In the deferred urgent group, the CBD and the CD were easily identified as first structure in a high percentage of cases (65.2% and 41.3% respectively), whereas the CD-CHD junction was the third structure to be identified in 67.4% of cases, the highest value among the three subgroups. In the difficult group, NIRF-C did not prove to be a useful tool for biliary visualization. The rates of failure of visualization were elevated: CBD (27.3%), CD (18.2%), CHD (54.5%) and CD-CHD (68.2%).
Conclusions
NIRF-C is a powerful real-time diagnostic tool to detect CBD and CD during minimally invasive cholecystectomy, especially when inflammation due to acute or chronic cholecystitis subverted the anatomy of the hepatoduodenal ligament.
Darboux transformation, soliton solutions of a generalized variable coefficients Hirota equation
Authors: Wang Dan , Liu Shuli , Han Wenjing , Zhu Mengkun , Wang Xiaoli 
Published: March 2023

Abstract
It is known that the varible coefficients Hirota equations have been widely studied in the propagating pulses amplificat or absorpt and the yield of supercontinuum in inhomogeneous optical fibers. In this paper, a new generalized case is considered, i.e.
iut + α(t)uxx + iβ(t)uxxx + 3iβ(t)γ|u|2ux + α(t)γ|u|2u + δ(t)u = 0 ,
where i = √ −1 indicates the imaginary unit and u is a complex function with the variables (t, x). In particular, for α, β, γ are all constants, δ = 0, the classical Hirota equation will be recovered. Firstly, we constructed the classical and generalized Darboux transformations of the equation, respectively. Next, multisoliton solutions are obtained based on classical Darboux transformation, as well as, rogue wave solutions are found by generalized Darboux transformation. Finally, we discussed the evolutions of solitons.
Worldwide productivity and research trend of publications concerning tumor immune microenvironment (TIME): a bibliometric study
Authors: Liu Yao-ge , Jiang Shi-Tao , Zhang Lei , Zheng Han , Zhang Ting , Zhang Jun-Wei , Zhao Hai-Tao , Sang Xin-Ting , Xu Yi-Yao , Lu Xin 
Published: March 2023

Abstract
Background: As the complexity and diversity of the tumor immune microenvironment (TIME) are becoming better understood, burgeoning researches got progress in this field. This study sought to investigate the development pattern of TIME-related research in recent years from a bibliometric perspective.
Methods: We acquired both articles and reviews related to TIME from the Web of Science Core Collection (WoSCC) (retrieved on September 14, 2022). R package “Bibliometrix” was used to calculate the basic bibliometric features, present the collaborative conditions of countries and authors and generate a three-field plot to show the relationships among authors, affiliations, and keywords. VOSviewer was utilized for co-authorship analysis of country and institution and keyword co-occurrence analysis. CiteSpace was used for citation burst analysis of keywords and cited references.
Results: A total of 2545 publications on TIME were included. China, the USA, and Japan had the highest number of publications. Frontiers in Oncology had the highest number of publications. Apart from “expression,” “cancer,” and “cells,” the terms “survival,” “immunotherapy,” and “T-cells” were coequally the most frequently mentioned keywords.
Conclusions: This bibliometric study analyzed 16 years of TIME-related research and sketched out a basic knowledge framework that includes publications, countries, journals, authors, institutions, and keywords. The results provided a thorough overview on the broad landscape of this research area.
Free Wi-Fi Calling Without Sim and Internet
Authors: Karsarpe Mahesh 
Published: March 2023

Abstract
Private businesses began to rely more and more on telephone communication as the contemporary telephone networks took shape. A lot of people choose to launch their own business. so that they could manage calls coming from within the company. Voice telephony on mobile devices is provided at a fee using a service provider like GSM, or at a lesser cost using an IP service provider. The purpose of this project is to build and implement a telephony programme that employs WLAN (Wireless Local Area Network) or Peer-to-Peer (P2P) WIFI as a free method of communication between mobile phones. Phone numbers will be converted into IP addresses by the 3CX Phone System software using a correlation between the most recent address books made available on mobile devices.The system will permit voice communications from users. The current setup does not support conference calls or call waiting, only one call per connection. We describe various security services that are appropriate to VoIP and make the case that end-to-end authentication and encryption ought to be offered by default.
Humic acid catalyzed solvent-free green protocol for synthesis of thioamide
Authors: Mukherjee Suvodip , Mitra Bijeta , Pariyar Gyan Chandra , Pal Sandipan , Deb Mayukh , Ghosh Pranab 
Published: March 2023

Abstract
An environmentally sustainable, green synthesis of thioamide through multi-component reaction (MCR) of aldehyde, amine and sulphur catalyzed by Humic acid in solvent-free condition at 100 oC. The key features of this protocol are use of humic acid, a greener, easily recyclable, easily available and almost unexplored catalyst and circumvention of noxious solvents that amplify the scope of the reaction. The proposed protocol also possesses tolerance to aromatic as well as aliphatic aldehydes and amines comprising variety electron donating and withdrawing functional groups.
Enhancing Cancer Treatment and Understanding Through Clustering of Gene Responses to Categorical Stressors
Authors: Hadi Christopher El , Hilal George , Aoun Rita 
Published: March 2023

Abstract
Cancer cells have unique metabolic activity in the glycolysis pathway compared to normal cells, which allows them to sustain their growth and proliferation. Therefore, inhibiting glycolytic pathways may provide a promising therapeutic approach to cancer treatment. In this first-of-its-kind study, we analyzed the genetic responses of cancer cells to stressors, particularly drugs that target the glycolysis pathway. Gene expression data for experiments on different types of cancer cells were retrieved from the Gene Expression Omnibus and expression fold-change was then clustered after dimensionality reduction. We identified four response clusters, the first and third are affected the most by anti-glycolytic drugs, consisting mainly of squamous and mesenchymal tissues, showing higher mitotic inhibition and apoptosis. Drugs acting on several glycolytic targets at once resulted in such responses. The second and fourth clusters were relatively unaffected by the treatments, succumbing the least to glycolysis inhibitors. These clusters are mainly gynecological and hormone-sensitive, with drugs acting on hexokinases mainly inducing this response. This study highlights the importance of analyzing the molecular states of cancer cells to identify potential targets for personalized cancer treatments and to improve our understanding of the disease.
Nutrient Uptake in Supplemented Substrate by Oyster Mushroom
Authors: Jesus Gabriel , Lavoranti Osmir , SCHAEFER Gilmar , OLIVEIRA Gabriel Dias , SILVA Ricardo Scheffer Andrade , Cuquel Francine 
Published: March 2023

Abstract
Spent mushroom substrate (SMS) can be a good alternative for the supplementation of the Oyster mushroom substrate, replacing the cereal bran normally used. Therefore, the objective was to evaluate the production of Pleurotus ostreatus supplemented with Lentinula edodes’ SMS, through the nutritional analysis of the substrate. Wheat straw was used as substrate and supplemented with rice bran (RB) or SMS in 0%, 7%,15% and 30%. Ca, K, Mg, Mn, Zn, Cu and Fe contents of the cultivation substrates (before and after harvest) were determined. Mycelial growth (cm²/day), mycelial time colonization (days), number of clusters, number of pileus, average clusters weight (g), pileus lenght (cm) and width (cm), productivity (1st, 2nd and 3rd flush) (%), biological efficiency (%) of mushrooms were evaluated. Results indicated mycelial growth was higher when the substrate was supplemented regardless of the source. The same tendency followed for second flush (%), third flush (%), average clusters weight (g), productivity (%) and biological efficiency (%). The proportions of 15% of SMS achieved the highest biological efficiency. All the nutrients analyzed had a decrease in their concentrations. The only nutrients that showed differences in absorption according to each substrate used were Ca, K and Mn. This difference was also noticed according to the type of supplement, in which substrates supplemented with SMS had greater absorption of Ca, while substrates supplemented with RB absorbed more K. It is noteworthy that the mineral contents in substrates directly affect the production of P. ostreatus, which implies that the SMS are a nutritional source in terms of mineral contents and thus can be recycled and used to replace rice bran and produce Oyster mushroom.