bluuebunny/crossref_metadata_2025_split huggingface dataset:
Burden of Liver Cancer in China from 1990 to 2019 and projections to 2044: Findings from the Global Burden of Disease Study
Authors: Han Jianglong , Chen Chao , Tang Tian , Chen Ruyan , Li Si , Deng Haiyu , Zhao Liang , Fu Zhenming 
Published: September 2023

Abstract
Background
China has the highest liver cancer burden in the world. Prediction and comparison of the future trends of liver cancer in China and some representative areas may guide further control action.
Methods
Using data from the Global Burden of Disease Study, we assessed incidence, mortality, and disability-adjusted life-years of liver cancer in Mainland China, with reference to representative East Asia areas (Taiwan China, Japan, and Korea) and Western areas (the United Kingdom and the United States). The burden of liver cancer was evaluated and predicted using NOREPRED model from 1990 to 2044.
Results
Overall, the liver cancer incidence (28.1 to 10.6 per 100,000) and mortality (27.5 to 9.7 per 100,000) decreased from 1990 to 2015 in Mainland China, which were consistent with the trends of Eastern Asia areas. However, the disease burden in Mainland China were then plateaued and started to increase during 2015–2044 (10.6 to 14.8 per 100,000 for incidence; 9.7 to 14.02 per 100,000 for mortality), including the hepatitis-related liver cancer incidence (increase from 8.6 to 11.7 per 100,000). While the changing patterns of alcohol- and nonalcoholic steatohepatitis (NASH)-related liver cancer incidence were found similar among Mainland China (0.93 to 1.51 per 100,000 for alcohol; 0.5 to 0.73 per 100,000 for NASH) and Western countries in our projection.
Conclusion
The liver cancer burden in Mainland China is unexpectedly predicted to increase again after decades of decline. Future efforts must be made to resolve both the remaining hepatitis-related cancer burden and the changing etiologies.
TOP1 and R-loops facilitate transcriptional DSBs at hypertranscribed cancer driver genes
Authors: Aqeilan Rami , Hidmi Osama , Oster Sara , Monin Jonathan 
Published: September 2023

AbstractDNA double-stranded breaks (DSBs) pose a significant threat to genomic integrity, and their generation during essential cellular processes like transcription remains poorly understood. In this study, we employed advanced techniques to map DSBs, R-loops, and Topoisomerase 1 cleavage complex (TOP1cc) and re-analyzed ChIP-seq and DRIP-seq data to comprehensively investigate the interplay between transcription, DSBs, Topoisomerase 1 (TOP1), and R-loops. Our findings revealed the presence of DSBs at highly expressed genes enriched with TOP1 and R-loops, indicating their crucial involvement in transcription-associated genomic instability. Depletion of R-loops and TOP1 specifically reduced DSBs at highly expressed genes, uncovering their pivotal roles in transcriptional DSB formation. By elucidating the intricate interplay between TOP1cc trapping, R-loops, and DSBs, our study provides novel insights into the mechanisms underlying transcription-associated genomic instability. Moreover, we establish a link between transcriptional DSBs and early molecular changes driving cancer development. Notably, our study highlights the distinct etiology and molecular characteristics of driver mutations compared to passenger mutations, shedding light on the potential for targeted therapeutic strategies. Overall, these findings deepen our understanding of the regulatory mechanisms governing DSBs in hypertranscribed genes associated with carcinogenesis, opening avenues for future research and therapeutic interventions.
Explanation of social support for labor children in Tehran: a qualitative study
Authors: Sahbaeiroy Faezeh , Koshanfar Ali 
Published: September 2023

Abstract
Introduction: Social support understanding can prevent the occurrence of adverse social effects, increase people's progress, leave a positive impact on the physical, mental, and social condition of people, and ultimately lead to an increase in their performance.
Research method: The present study was a qualitative type, with a content analysis approach; this research was conducted in the workplace and where the participants felt comfortable. To this end, semi-structured interviews were conducted with 8 experts on the issue of labor children, The collection tool was an interview guide, and the participants were selected based on purposeful sampling among experts and decision-makers in the matter of labor children. The process of selecting participants continued until no new information was obtained in the interviews and the data reached saturation. Then the interviews along with the desired codes were entered into MAXQDA version 10 software and data analysis was done.
Findings: From the total interviews, 10386 initial codes, 265 overlapped initial codes 67 classes, and 19 merging data and five axial codes including lack of responsibility, unfair distribution of the country's wealth, lack of social support, increasing unemployment in the country and also Non-application of child rights laws were found. "Lack of responsibility of an individual or organization"was identified as the selective code of the participants in the research and the main variable of the research, which connected all the variables or classes with this concern.
Conclusion: managers and broker's responsibility is to protect people and defend their rights. Accountability and transparency are one of the main tools against corruption and achieving an accountable system is one of the main goals of any country. The existence of an efficient and accountable system helps maintain the acceptability of the political system and on the other hand, it can prevent corruption's growth.
Selective Aortic Arch Perfusion: A First-in-Man Report of a Novel Endovascular Resuscitation Modality
Authors: Marsden Max E R , Barratt Jon , Donald-Simpson Helen , Wilkinson Tracey , Manning Jim , Rees Paul 
Published: September 2023

Abstract
Background
Selective aortic arch perfusion (SAAP) is a novel endovascular technique that combines thoracic aortic occlusion with extracorporeal perfusion of the brain and heart. SAAP may have a role in both haemorrhagic shock and in cardiac arrest due to coronary ischaemia. Despite promising animal studies, no data is available that describes SAAP in humans. The primary aim of this study was to assess the feasibility of selective aortic arch perfusion in humans. The secondary aim of the study was to assess the feasibility of achieving direct coronary artery access via the SAAP catheter as a potential conduit for salvage percutaneous coronary intervention.
Methods
Using perfused human cadavers, a prototype SAAP catheter was inserted into the descending aorta under fluoroscopic guidance via a standard femoral percutaneous access device. The catheter balloon was inflated and the aortic arch perfused with radio-opaque contrast. The coronary arteries were cannulated through the SAAP catheter.
Results
The procedure was conducted four times. During the first two trials the SAAP catheter was passed rapidly and without incident to the intended descending aortic landing zone and aortic arch perfusion was successfully delivered via the device. The SAAP catheter balloon failed on the third trial. On the fourth trial the left coronary system was cannulated using a 5Fr coronary guiding catheter through the central SAAP catheter lumen.
Conclusions
For the first time using a perfused cadaveric model we have demonstrated that a SAAP catheter can be easily and safely inserted and SAAP can be achieved using conventional endovascular techniques. The SAAP catheter allowed successful access to the proximal aorta and permitted retrograde perfusion of the coronary and cerebral circulation.
MouseGoggles: an immersive virtual reality headset for mouse neuroscience and behavior
Authors: Isaacson Matthew , Chang Hongyu , Berkowitz Laura , Zirkel Rick , Park Yusol , Hu Danyu , Ellwood Ian , Schaffer Chris 
Published: September 2023

Abstract

We present MouseGoggles, a miniaturized virtual reality (VR) display for head-fixed mice that delivers independent, binocular visual stimulation over a wide field of view. Neural recordings in the visual cortex validate the quality of image presentation, while hippocampal recordings, associative reward learning, and innate fear responses demonstrate an immersive VR experience. Our open-source system’s simplicity, low cost, and compact size will allow for broader adoption of VR methods in neuroscience.
Mother-offspring chemical communication and tadpole aggregation in a Neotropical foam-nesting frog
Authors: Carrillo JUAN F C , Ortega Zaida , Santana Diego J , Prado Cynthia P A 
Published: September 2023

Abstract

Communication during parental care represents an advantage for eliciting offspring behaviour and sibling recognition. In aquatic environments, chemical communication is usually the most effective, playing different roles in amphibian parental behaviour. Attending females of some Neotropical anurans (Leptodactylus) lead entire tadpole schools through the water. However, little is known about this aggregation behaviour, such as the type of signal used by attending females or if they are specific between mothers and offspring. Here we conducted behavioural experiments to investigate these questions. Firstly, we tested the effect of visual, tactile, and chemical stimulus of attending females on tadpole aggregation behaviour. Following, we compared the effects of the chemical stimulus from attending females with those from other conspecifics (non-attending females and males) on schooling behaviour. Finally, we tested whether tadpoles prefer related attending females instead of unrelated ones. Our results indicated that only chemical stimulus from attending females, and not visual or tactile, increased tadpoles´ aggregation time. Chemical stimulus from non-attending females and males did not affect tadpoles’ behaviour. Contrary to what we expected, we did not find school preference for related attending females. We were able to demonstrate the presence of chemical signals in mother-tadpole school relationship of Leptodactylus species. Besides, we showed that chemical signals both attract and aggregate tadpoles, which can be crucial for attending females, who guide the entire school through aquatic habitats. Furthermore, our results suggest that tadpoles do not prefer related attending females and may benefit from following unrelated attending female tacking advantage of alloparental care.
The use of a systems approach to increase NAD+ in human participants
Authors: Ford Dianne , Henderson John , Quigley Sophia , Chachra Shruti , Conlon Nichola 
Published: September 2023

Abstract
Reversal or mitigation against an age-related decline in NAD+ has likely benefits, and this premise has driven academic and commercial endeavour to develop dietary supplements that achieve this outcome. We used a novel systems-based approach to improve on current supplements by targeting multiple points in the NAD+ salvage pathway. In a double-blind, randomised, crossover trial, the supplement – Nuchido TIME+® (NT) - increased NAD+ concentration in whole blood. This was associated with an increase in SIRT1 and an increase in nicotinamide phosphoribosyltransferase (NAMPT) in peripheral blood mononucleocytes, lower concentrations of pro-inflammatory cytokines in plasma, including a reduction in interleukin 2 (IL2), a reduction in glycated serum protein and a shift in the glycosylation profile of immunoglobulin G (IgG) toward a younger biological age, all of which are likely to promote a healthier aging trajectory.
Area Under the Inspiratory Flow-Volume Curve (AIX): Proposed Normative Values
Authors: Ioachimescu Octavian , Stoller James K. 
Published: September 2023

Abstract
Area under the inspiratory flow-volume loop (AIX) is a spirometric tool that is potentially useful in characterizing upper and/or lower airflow obstruction. We analyzed AIX in 4,980 single-test, pre- or post-bronchodilator normal spirometry tests recorded in non-smoking, healthy individuals in the Pulmonary Function Testing Laboratory. The mean (95% confidence interval, CI), standard deviation and median (25th -75th interquartile range) AIX were 16.05 (15.79–16.31), 9.08 and 14.72 (9.12–21.42) L2⋅sec− 1, respectively. The mean (95% CI) and standard deviation of the best-trial measurements for square root of AIX (SqrtAIX, which follow a distribution closer to a gaussian distribution, were 3.84 (3.81–3.87) and 1.14; 4.15 (4.12–4.18) and 1.03 in men, and 2.68 (2.63–2.72) and 0.72 L⋅sec− 1/2 in women. The mean (standard deviation) of pre- and post-bronchodilator SqrtAIX were 3.71 (1.17) and 3.81 (1.19) L⋅sec− 1/2, respectively. The mean (95% CI), standard deviation and lowest 5th percentile (lower limit of normal, LLN) of SqrtAIX/SqrtAEX (%) were 101.3 (100.82-101.87), 18.7%, and 71.8%; stratified by gender, it was 102.2 (101.6-102.8), 18.6, and 72.8% in men, and 98 (96.9–99.2), 18.8, and 68.6% in women, respectively. The availability of area under the inspiratory flow-volume curve (AIX) and the derived indices offers a promising opportunity to assess upper airway disease (e.g., involvement of larynx, trachea or major bronchi), especially because some of these measurements appear to be independent of age, race, height, and weight.
Transcriptome Analysis Identified Dichogamy Regulation Networks in Alpinia mutica
Authors: Huang Ao-Dan , Peng Xiao-Chang , Duan Sheng-Chang , Jiang Li-Ju , Xiang Gui-Sheng , Zhao Jian-Li , Li Qing-Jun , Dong Yang , Wang Wei-Bin 
Published: September 2023

Abstract
In the sexual reproduction of flowering plants, outcrossing has natural advantages in heredity, and is the reproduction method used by most flowering plants. Dichogamy, accompanied by flower sex transition or differential maturation, promotes outcrossing and reduces selfing and interference with male and female functions, impacting the diversity and evolution of flowering plants significantly. However, due to a chronic lack of available research materials, the regulatory mechanism of dichogamy, an important reproductive trait that has been of great interest since Darwin's time, remains unknown. Fortunately, Aplinia mutica, a species of wild ginger that has two sexes within the same population and achieve dichogamy through sex transition, provides superb research material. In this study, differential expression gene analysis and group screening were conducted to identify the underlying causes and potential regulatory networks that may be involved in the flower sex transition and the maturity of two sex organs, anther, and stigma. Our findings revealed that genes such as MYB-TFs, NAC-TFs, FLA11, KSC6, NADPH, PP1CG2, and VTC2; plant hormones such as jasmonic acid (JA), auxin, and brassinosteroids (BR); environmental factors such as light and temperature; and many other factors, such as phosphorylation and other biological processes, may all affect the sex transition process of A. mutica. the regulation of sex transition and dichogamy is a complex process. Since the regulatory mechanism of dichogamy has been an important open question for a long time, our study has made a novel contribution by identifying and examining its transcriptional regulation in plants at the molecular level. This finding holds immense importance for further investigations in this area.
A gut microbial metabolite of linoleic acid ameliorates liver fibrosis by inhibiting TGF-β signaling in hepatic stellate cells
Authors: Kasahara Nanaho , Imi Yukiko , Amano Reina , Shinohara Masakazu , Okada Kumiko , Hosokawa Yusei , Imamori Makoto , Tomimoto Chiaki , Kunisawa Jun , Kishino Shigenobu , Ogawa Jun , Ogawa Wataru , Hosooka Tetsuya 
Published: September 2023

Abstract
The antidiabetic drug pioglitazone ameliorates insulin resistance by activating the transcription factor PPARγ. In addition to its blood glucose–lowering action, pioglitazone exerts pleiotropic effects including amelioration of nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH). The mechanism by which pioglitazone achieves this latter effect has remained unclear, however. We here show that pioglitazone administration increases the amount of linoleic acid (LA) metabolites in adipose tissue of KK-Ay mice. These metabolites are produced by lactic acid bacteria in the gut, and pioglitazone also increased the fraction of Lactobacillus in the gut microbiota. Administration of the LA metabolite HYA (10-hydroxy-cis-12-octadecenoic acid) to C57BL/6J mice fed a high-fat diet improved liver histology including steatosis, inflammatory cell infiltration, and fibrosis. Gene ontology analysis of RNA-sequencing data for the liver revealed that the top category for genes downregulated by HYA treatment was related to extracellular matrix, and the expression of individual genes related to fibrosis was confirmed to be attenuated by HYA treatment. Mechanistically, HYA suppressed TGF-β–induced Smad3 phosphorylation and fibrosis-related gene expression in human hepatic stellate cells (LX-2). Our results implicate LA metabolites in the mechanism by which pioglitazone ameliorates liver fibrosis, and they suggest that HYA is a potential therapeutic for NAFLD/NASH.
Age-specific comparisons in the rate of force development of toe pressure strength and its association with the Timed Up and Go test
Authors: Kamasaki Taishiro , Otao Hiroshi , Tanaka Shinichi , Hachiya Mizuki , Kubo Atsuko , Okawa Hiroyuki , Sakamoto Asuka , Fujiwara Kazuhiko , Suenaga Takuya , Kichize Yo , Shimokihara Suguru , Maruta Michio , Han Gwanghee , Mizokami Yasuhiro , Tabira Takayuki 
Published: September 2023

Abstract
Purpose
It has recently been recommended that Rate of Force Development (RFD) be evaluated in addition to maximal muscle strength. There are no studies on RFD of toe pressure strength, and its importance in older adults and the extent to which it is associated with aging needs to be clarified. This study purpose was to examine the association between the RFD of toe pressure strength and Timed Up and Go Test (TUG) in an age-specific study.
Methods
This study is a cross-sectional study. Participants in the study included 159 healthy adults (26.3 ± 13.1 years, 52% male) and 88 older adults (75.0 ± 6.2 years, 26% male). The RFD of toe pressure strength was determined from the force-time curve obtained during the toe pressure strength assessment, and the ability to exert maximum muscle force in the shortest possible time was assessed. Regression analysis was performed for each group to test the association between RFD of toe pressure strength and TUG by age.
Results
Healthy adults showed no association between TUG and RFD of toe pressure strength, and significant association between TUG and RFD of toe pressure strength was found only in the older adults (standard regression coefficient = − 0.15, p = 0.048).
Conclusion
This study showed a significant association between TUG and RFD of toe pressure strength in older adults. These findings show that RFD is one of the functions that should be assessed, particularly in older adults. Furthermore, it was suggested that approaching RFD could improve gait, standing, and sitting movements.
Mushroom bioreactor-biomass as bioactive protein source: Synergy of mushroom rural and urban cultivation
Authors: Wan-Mohtar Wan Abd Al Qadr Imad , Azizan Nur Asyiqin Zahia , Tan Rui Yeong , Ilham Zul , Jamaludin Adi Ainurzaman 
Published: September 2023

Abstract
Mushroom rural cultivation consumes averagely 6-month period, while urban cultivation takes only 10 days or less. In this study, mushroom biomass was grinded and converted into a flour to produce mushroom-chicken patties using Lingzhi and Enoki. The inclusion of Enoki in chicken patties (10%, 20% and 30%) indicates higher consumer acceptance significantly (p > 0.05) compared to chicken patties with Lingzhi (10% and 20%). This analysis validated the concept of mushroom biomass as source of bioactive protein. On the other hand, 3kg dried mushroom-bioreactor biomass was produced using a heterotrophic 1m² fabricated-bioreactor, which answers the minimum requirement for protein content for 1 human per year. Together, these explain the significance of mushroom biomass in food security as a protein source and the synergy of mushroom rural-urban cultivation.
Interplay between Hole Superconductivity and Quantum Critical Antiferromagnetic Fluctuations in Electron-Doped Cuprates
Authors: Kim Changyoung , Song Dongjoon , Lee Suheon , Shen Zecheng , Jung Woobin , Lee Wonjun , Choi Sungkyun , Kyung Wonshik , Jung Saegyeol , Ishida Shigeyuki , Yoshida Yoshiyuki , Park Seung Ryong , Eisaki Hiroshi , Wang Yao , Choi Kwang-Yong 
Published: September 2023

Abstract
Antiferromagnetic spin fluctuations are the most promising candidate as the pairing glue of high critical temperature (Tc) superconductivity. However, many-body states and intertwined orders in cuprates have made it difficult to determine how electrons interact with fluctuating spins to form Cooper pairs. Recent experimental and theoretical studies have suggested spin fluctuation-driven quasiparticle band folding, but the relationship between the resultant Fermi pockets and superconductivity remains unclear. Here, we investigated this relationship in electron-doped Pr1−xLaCexCuO4±δ using angle-resolved photoemission and muon spin spectroscopy. By extracting the folded band component in the single-particle nodal band spectrum and analysing the muon relaxation rate, we discovered that Tc is proportional to the quasiparticle weight of the nodal hole pocket in the regime of the fluctuating antiferromagnetic ground state around a presumed quantum critical point. Our experimental and numerical observations highlight the significance of the marked interplay between the electron correlation and antiferromagnetic fluctuations in enhancing the hole pocket and consequently driving superconductivity.
The Role of Small RNAs in Resistant Melon Cultivar against Phelipanche aegyptiaca Parasitization
Authors: Mao Jiancai , Wang Haojie , Yan Miao , Guo Yao , Zhao Kaige , Zhang Hong , Yang Junyan , Li Junhua , Zhai Wenqiang 
Published: September 2023

Abstract

Bidirectional trans-kingdom RNA silencing, a pivotal factor in plant-pathogen interactions, remains less explored in plant host-parasite dynamics. Here, using small RNA sequencing in melon root systems, we investigated miRNA expression variation in resistant and susceptible cultivars pre- and post-infection by the parasitic plant, broomrape. This approach revealed 979 known miRNAs and 110 novel miRNAs across 110 families. When comparing susceptible (F0) and resistant (R0) melon lines with broomrape infection (F25 and R25), 39 significantly differentially expressed miRNAs were observed in F25 vs. F0, 35 in R25 vs. R0, and 5 in R25 vs. F25. Notably, two miRNAs consistently exhibited differential expression across all comparisons, targeting genes linked to plant disease resistance. This suggests their pivotal role in melon's defense against broomrape. The target genes of these miRNAs were confirmed via degradome sequencing and validated by qRT-PCR, ensuring reliable sequencing outcomes. GO and KEGG analyses shed light on the molecular functions and pathways of these differential miRNAs. Furthermore, our study unveiled four trans-kingdom miRNAs, forming a foundation for exploring melon's resistance to broomrape.