bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical features of Streptococcus intermedius infection in children: a case series study
Authors: Xu Zhufei , Gao Lichao , Xu Dan , Yang Dehua , Chen Zhimin , Wang Yingshuo 
Published: August 2023

IntroductionStreptococcus intermedius is an opportunistic pathogen associated with prolonged hospital stays and high mortality rates in adults. However, little is currently known about the clinical features of Streptococcus intermedius infection in children.MethodsThis retrospective case series study included 40 children diagnosed with Streptococcus intermedius, confirmed through bacterial cultures or high-throughput sequencing. Antibiotic resistance was assessed through susceptibility testing. The site and clinical manifestations were evaluated for all patients.ResultsThe common infection sites were the abdominal cavity, skin and soft tissue, intracranial, and invasive pulmonary, with the abdominal cavity being the most frequently affected. The drug susceptibility test showed 100% sensitivity to ceftriaxone, levofloxacin, chloramphenicol, vancomycin, and linezolid, 92.6% sensitivity to penicillin, 73.3% resistance to erythromycin, and 76.7% resistance to clindamycin. Besides antibiotic therapy, surgical intervention or pus drainage was often necessary. Lung imaging of four patients revealed pulmonary abscesses, nodules, or encapsulated pleura. Two cases yielded positive culture results, while three were identified as positive through high-throughput nucleotide sequencing of pleural effusion.DiscussionIn children with Streptococcus intermedius infection, emphasis should be placed on the risk of pus or abscess formation. In cases of pulmonary abscess and pleural effusion, especially in male children, Streptococcus intermedius should be suspected even if the culture is negative. Improvements in high-throughput nucleotide sequencing are required to reduce misdiagnosis rates.
Effect of swab pooling on the Accula point-of-care RT-PCR for SARS-CoV-2 detection
Authors: Lancelot Moira , Fibben Kirby , Sullivan Julie , O’Sick William , McLendon Kaleb , Wu Huixia , Rao Anuradha , Bassit Leda C. , Greenleaf Morgan , Miller Pamela , Krull Wolfgang , Tyburski Erika , Roback John D. , Lam Wilbur A. , Damhorst Gregory L. 
Published: August 2023

IntroductionSwab pooling may allow for more efficient use of point-of-care assays for SARS-CoV-2 detection in settings where widespread testing is warranted, but the effects of pooling on assay performance are not well described.MethodsWe tested the Thermo-Fisher Accula rapid point-of-care RT-PCR platform with contrived pooled nasal swab specimens.ResultsWe observed a higher limit of detection of 3,750 copies/swab in pooled specimens compared to 2,250 copies/swab in individual specimens. Assay performance appeared worse in a specimen with visible nasal mucous and debris, although performance was improved when using a standard laboratory mechanical pipette compared to the transfer pipette included in the assay kit.ConclusionClinicians and public health officials overseeing mass testing efforts must understand limitations and benefits of swab or sample pooling, including reduced assay performance from pooled specimens. We conclude that the Accula RT-PCR platform remains an attractive candidate assay for pooling strategies owing to the superior analytical sensitivity compared to most home use and point-of-care tests despite the inhibitory effects of pooled specimens we characterized.
DFA as a window into postural dynamics supporting task performance: does choice of step size matter?
Authors: Nordbeck Patric C. , Andrade Valéria , Silva Paula L. , Kuznetsov Nikita A. 
Published: August 2023

Introduction: Detrended Fluctuation Analysis (DFA) has been used to investigate self-similarity in center of pressure (CoP) time series. For fractional gaussian noise (fGn) signals, the analysis returns a scaling exponent, DFA-α, whose value characterizes the temporal correlations as persistent, random, or anti-persistent. In the study of postural control, DFA has revealed two time scaling regions, one at the short-term and one at the long-term scaling regions in the diffusion plots, suggesting different types of postural dynamics. Much attention has been given to the selection of minimum and maximum scales, but the choice of spacing (step size) between the window sizes at which the fluctuation function is evaluated may also affect the estimates of scaling exponents. The aim of this study is twofold. First, to determine whether DFA can reveal postural adjustments supporting performance of an upper limb task under variable demands. Second, to compare evenly-spaced DFA with two different step sizes, 0.5 and 1.0 in log2 units, applied to CoP time series.Methods: We analyzed time series of anterior-posterior (AP) and medial-lateral (ML) CoP displacement from healthy participants performing a sequential upper limb task under variable demand.Results: DFA diffusion plots revealed two scaling regions in the AP and ML CoP time series. The short-term scaling region generally showed hyper-diffusive dynamics and long-term scaling revealed mildly persistent dynamics in the ML direction and random-like dynamics in the AP direction. There was a systematic tendency for higher estimates of DFA-α and lower estimates for crossover points for the 0.5-unit step size vs. 1.0-unit size.Discussion: Results provide evidence that DFA-α captures task-related differences between postural adjustments in the AP and ML directions. Results also showed that DFA-α estimates and crossover points are sensitive to step size. A step size of 0.5 led to less variable DFA-α for the long-term scaling region, higher estimation for the short-term scaling region, lower estimate for crossover points, and revealed anomalous estimates at the very short range that had implications for choice of minimum window size. We, therefore, recommend the use of 0.5 step size in evenly spaced DFAs for CoP time series similar to ours.
JK5G postbiotics attenuate immune-related adverse events in NSCLC patients by regulating gut microbiota: a randomized controlled trial in China
Authors: Chen Mengting , Ma Liling , Yu Huiqing , Huang Shaoyi , Zhang Junhui , Gong Juan , Yang Liejun , Chen Lan , Luo Haojun , Tian Ling , Wang Sixiong 
Published: August 2023

ScopeThis study aimed to evaluate the effects of JK5G postbiotics to regulate imbalanced gut microbiota and its impacts on the efficacy and incidence rate of immune-related adverse events (irAEs) in non-small-cell lung cancer (NSCLC) patients treated with immune checkpoint inhibitors (ICIs).MethodsThis randomized, double-blind, placebo-controlled trial was conducted in China and included non-squamous or squamous NSCLC patients without EGFR, ROS1, and ALK alteration, treatment-naive, and stage IIIb-IV. Patients were randomly (1:1) divided into two groups to receive four cycles (three weeks for each cycle) of programmed cell death-1 (PD-1) plus chemotherapy plus placebo (control group, n = 30) or to receive PD-1 plus chemotherapy plus JK5G postbiotics (JK5G group, n = 30). The primary endpoint was objective response rate. The secondary endpoints were quality of life (QoL), adverse effects, and the 16S DNA sequencing of gut microbiota, blood inflammatory cytokines, and lymphocyte subsets. This study was registered at www.chictr.org.cn (ChiCTR2200064690).ResultsSixty patients were enrolled. The objective response rate was 36.67% (11/30) in the control group and 50.00% (15/30) in the JK5G group (p = 0.297). The JK5G group had better QoL and nutritional levels, as well as lower depression symptoms than the control group (all p &lt; 0.05). Moreover, the JK5G group had a lower incidence of anemia (63.33% vs. 13.33%, p &lt; 0.001), decreased lymphocyte count (20.00% vs. 0%, p = 0.010), decreased appetite (53.33% vs. 16.67%, p = 0.003), nausea (33.33% vs. 6.67%, p = 0.010), and asthenia (30.00% vs. 6.67%, p = 0.017) than the control group. Moreover, JK5G attenuated gut microbiota imbalance, accompanied by increased Faecalibacterium, Ruminococcaceae, and fecal butyrate concentration, and diminished Escherichia-Shigella. Furthermore, JK5G administration significantly decreased the levels of pro-inflammatory markers, including TNF-α, IL-2, and C-reactive protein (CRP) (all p &lt; 0.05). Significant increases in CD3+CD4+ T cells and CD4/CD8 ratio were observed in the peripheral blood of JK5G group patients (all p &lt; 0.05). The enterotype data showed that patients were clustered into Blautia (E1) and Escherichia-Shigella (E2) enterotypes, and JK5G postbiotics intervention might be related to enterotype modulations.ConclusionOur current findings indicated that JK5G postbiotics might attenuate irAEs, and enhance the QoL and nutrition levels of advanced NSCLC patients who received ICIs. JK5G postbiotics could also improve the gut microbiota structures and ameliorate the tumor microenvironment and inflammation.Clinical trial registrationwww.chictr.org.cn, identifier ChiCTR2200064690.
Comparison of SARS-CoV-2 seroconversion in children with chronic diseases with healthy children and adults during the first waves of the COVID-19 pandemic
Authors: Hoste Levi , Prytula Agnieszka , Dehoorne Jo , De Bruyne Ruth , Van Biervliet Stephanie , De Waele Kathleen , Maes Evelyn , Bordon Victoria , Vanlander Arnaud , Claes Karlien , Vande Walle Johan , Schelstraete Petra , Van daele Sabine , Haerynck Filomeen 
Published: August 2023

BackgroundInfection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is clinically diverse, and children have a low risk of developing severe coronavirus disease 2019 (COVID-19). However, children with chronic diseases have a potentially increased risk.MethodsWe performed a prospective surveillance study with longitudinal serum SARS-CoV-2 anti-nucleocapsid antibody quantification and questionnaires in pediatric tertiary care patients during the first waves of the COVID-19 pandemic (November 2020–September 2021). The results were compared with those of healthy children and adults from the same geographic area.ResultsWe obtained 525 samples from 362 patients (M/F ratio of 1.3:1; median age of 11.1 years) comprising children with immune-suppressive or immune-modulating drugs (32.9%), inborn errors of immunity (23.5%), type 1 diabetes mellitus (15.2%), and rheumatic diseases (11.9%). A total of 51 (9.7%) samples were seropositive among 37/351 children (10.5%). Seropositivity increased from 5.8% in November–December 2020 to 21.6% in July–September 2021. Compared with adults, a longitudinal analysis revealed reduced seroprevalence but similar kinetics as in children from the same country. Demographic or social variables and disease characteristics did not correlate with seropositivity. Being obese and household contact with COVID-19-infected individuals significantly increased the odds of infection. The majority of seropositive patients had mild symptoms (21/37). One-third were asymptomatic and/or unaware of having COVID-19 (10/37). Four patients (4/37) needed hospitalization, with good clinical outcomes.ConclusionsAlthough harboring a chronic disease, we observed a low SARS-CoV-2 incidence in a cohort of pediatric tertiary care patients, comparable with healthy children during the first year of the pandemic. Infection was mostly associated with mild symptoms.
Development of a small cell lung cancer organoid model to study cellular interactions and survival after chemotherapy
Authors: Sen Chandani , Koloff Caroline R. , Kundu Souvik , Wilkinson Dan C. , Yang Juliette M. , Shia David W. , Meneses Luisa K. , Rickabaugh Tammy M. , Gomperts Brigitte N. 
Published: August 2023

Introduction: Small-cell-lung-cancer (SCLC) has the worst prognosis of all lung cancers because of a high incidence of relapse after therapy. While lung cancer is the second most common malignancy in the US, only about 10% of cases of lung cancer are SCLC, therefore, it is categorized as a rare and recalcitrant disease. Therapeutic discovery for SCLC has been challenging and the existing pre-clinical models often fail to recapitulate actual tumor pathophysiology. To address this, we developed a bioengineered 3-dimensional (3D) SCLC co-culture organoid model as a phenotypic tool to study SCLC tumor kinetics and SCLC-fibroblast interactions after chemotherapy.Method: We used functionalized alginate microbeads as a scaffold to mimic lung alveolar architecture and co-cultured SCLC cell lines with primary adult lung fibroblasts (ALF). We found that SCLCs in the model proliferated extensively, invaded the microbead scaffold and formed tumors within just 7 days. We compared the bioengineered tumors with patient tumors and found them to recapitulate the pathology and immunophenotyping of the patient tumors. When treated with standard chemotherapy drugs, etoposide and cisplatin, we observed that some of the cells survived the chemotherapy and reformed the tumor in the organoid model.Result and Discussion: Co-culture of the SCLC cells with ALFs revealed that the fibroblasts play a key role in inducing faster and more robust SCLC cell regrowth in the model. This is likely due to a paracrine effect, as conditioned media from the same fibroblasts could also support this accelerated regrowth. This model can be used to study cell-cell interactions and the response to chemotherapy in SCLC and is also scalable and amenable to high throughput phenotypic or targeted drug screening to find new therapeutics for SCLC.
Lippia javanica (Burm. F.) Herbal Tea: Modulation of Hepatoprotective Effects in Chang Liver Cells via Mitigation of Redox Imbalance and Modulation of Perturbed Metabolic Activities
Authors: Salau Veronica F. , Erukainure Ochuko L. , Olofinsan Kolawole A. , Schoeman Recardia L. S. , Matsabisa Motlalepula G. 
Published: August 2023

Introduction:Hepatic oxidative injury is one of the pathological mechanisms that significantly contributes to the development of several liver diseases. In the present study, the hepatoprotective effect ofLippia javanicaherbal tea was investigated in Fe2+- mediated hepatic oxidative injury.Methods:Using anin vitroexperimental approach, hepatic oxidative injury was induced by co-incubating 7 mM FeSO4with Chang liver cells that have been pre-incubated with or without different concentrations (15–240 μg/mL) ofL. javanicainfusion. Gallic acid and ascorbic acid served as the standard antioxidants.Results:The infusion displayed a reducing antioxidant activity in ferric-reducing antioxidant power (FRAP) assay and a potent scavenging activity on 2,2-diphenyl-2- picrylhydrazyl (DPPH) radical. Pretreatment withL. javanicainfusion significantly elevated the levels of reduced glutathione and non-protein thiol, and the activities of superoxide dismutase (SOD) and catalase, with concomitant decrease in hepatic malondialdehyde levels, acetylcholinesterase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, glycogen phosphorylase and lipase activities. The infusion showed the presence of phytoconstituents such as phenolic compounds, tannins, phenolic glycosides and terpenoids when subjected to liquid chromatography—mass spectrometry analysis. Molecular docking revealed a strong binding affinity of dihydroroseoside and obacunone with both SOD and catalase compared to other phytoconstituents.Conclusion:These results portray a potent antioxidant and hepatoprotective effect ofL. javanica, which may support the local usage of the herbal tea as a prospective therapeutic agent for oxidative stress-related liver diseases.
Controlling nutritional status score is associated with renal progression, cardiovascular events, and all-cause mortality in biopsy-proved diabetic kidney disease
Authors: Huo Qingyu , He Ting , Xiong Jiachuan , Zhao Jinghong 
Published: August 2023

Background: The Controlled Nutritional Status (CONUT) score, calculated from albumin, total cholesterol, and lymphocyte count, is a useful indicator for immune-nutritional assessment and is associated with the prognosis of various diseases. However, its relationship with renal outcomes, cardiovascular disease (CVD), and all-cause mortality in patients with diabetic kidney disease is unclear.Methods: This retrospective single-center study enrolled 336 patients with biopsy-confirmed diabetic kidney disease from August 2009 to December 2018. The outcomes were progression to end-stage renal disease (ESRD), CVD events, and death. Univariate and multivariate Cox regression analyses were performed to estimate the association between confounding factors and outcomes. The Kaplan-Meier curve was used to compare the outcomes of the patients according to the median CONUT score. The area under the curve (AUC) evaluated with time-dependent receiver operating characteristics was used to test discriminative power of COUNT score.Results: During a median follow-up period of 5.1 years. The Kaplan-Meier analysis showed that patients in the high CONUT group (CONUT score &gt; 3) had a significantly higher incidence of ESRD, CVD events, and all-cause mortality than those in the low CONUT group (CONUT score ≤ 3). The multivariate COX regression analysis indicated that, The CONUT score was an independent predictor of ESRD (hazards ration [HR] = 1.129, 95% confidence interval [CI] 1.037-1.228, p = 0.005), CVD events (HR = 1.159, 95% CI 1.057-1.271, p = 0.002), and all-cause mortality (HR = 1.299, 95% CI 1.143-1.478, p &lt; 0.001).Conclusion: The CONUT score is an independent risk factor for ESRD, CVD events, and overall death in patients with diabetic kidney disease.
Leadership cycles, styles, and antecedent factors: the perspective of coaches and young soccer athletes from national Slovak leagues
Authors: Lisá Elena , Sousa Jacinta , Morais Catarina , Gomes António Rui 
Published: August 2023

IntroductionBuilding positive relationships and interactions between coaches and athletes is critical to an athlete’s success. The current study aimed to overview how coaches and their young athletes perceive three elements of the Leadership Efficacy Model (philosophy, practice, and criteria). The aim was examined with four goals of analysis: the perceptions of coaches and athletes about coaches’ leadership philosophy, practice, and criteria (1); the differences between athletes’ and coaches’ perceptions of leadership cycles (2); the differences between athletes’ and coaches’ perceptions of leadership styles (3); and the differences between athletes’ and coaches’ perceptions of leadership antecedent factors (4).MethodsThe study involved 304 athletes and 20 coaches competing in the youth national leagues U15, U16, U17, and U19. Two-source data collection was applied: coaches completed the questionnaires from their point of view, and so did athletes. The coaches were paired then with their athletes to compare the answers. Coaches fulfilled Leadership Cycles Questionnaire (LEQ), Multidimensional Scale of Leadership in Sport (MSLS), and Leadership Antecedent Factors Questionnaire (LAFQ). Athletes completed the same questionnaires as the coaches did and also fulfilled the Sport Performance Perception Questionnaire (SPPQ). Athletes’ age and SPPQ served as control variables.ResultsBoth athletes (37.5%) and coaches (40%) perceived that the philosophy of the leadership efficacy model should be increased. Coaches evaluated their philosophy (F = 4.43; p = 0.036; η2 = 0.014), support in MSLS (F = 5.05; p = 0.025; η2 = 0.016) and active management in MSLS (F = 4.08; p = 0.044; η2 = 0.013) higher than their athletes. The athletes assessed the maturity of the team members (LAFQ dimension) (F = 13.98; p &lt;0.001; η2 = 0.044), negative feedback in MSLS (F = 6.02; p = 0.015; η2 = 0.020), and passive management in MSLS (F = 4.95; p = 0.027; η2 = 0.016) higher than their coaches.DiscussionThe tendency of coaches to have a more positive perception of their leadership behavior compared to their athletes represents the coach-athlete perception gap of leadership. Future research can examine the efficacy of congruent perceptions of leadership between athletes and coaches during the sports season and the impact produced by objective performance indicators.
The multicenter effectiveness study of inpatient and day hospital treatment in departments of psychosomatic medicine and psychotherapy in Germany
Authors: Doering Stephan , Herpertz Stephan , Pape Magdalena , Hofmann Tobias , Rose Matthias , Imbierowicz Katrin , Geiser Franziska , Bierling Antonie Louise , Weidner Kerstin , Rademacher Jörg , Michalek Silke , Morawa Eva , Erim Yesim , Teigelack Per , Teufel Martin , Hartmann Armin , Lahmann Claas , Peters Eva Milena Johanne , Kruse Johannes , von Boetticher Dirk , Herrmann-Lingen Christoph , Nöhre Mariel , de Zwaan Martina , Dinger Ulrike , Friederich Hans-Christoph , Niecke Alexander , Albus Christian , Zwerenz Rüdiger , Beutel Manfred , Sattel Heribert Christian , Henningsen Peter , Stein Barbara , Waller Christiane , Hake Karsten , Spitzer Carsten , Stengel Andreas , Zipfel Stephan , Weimer Katja , Gündel Harald , Kessler Henrik 
Published: August 2023

BackgroundReliable outcome data of psychosomatic inpatient and day hospital treatment with a focus on psychotherapy are important to strengthen ecological validity by assessing the reality of mental health care in the field. This study aims to evaluate the effectiveness of inpatient and day hospital treatment in German university departments of Psychosomatic Medicine and Psychotherapy in a prospective, naturalistic, multicenter design including structured assessments.MethodsStructured interviews were used to diagnose mental disorders according to ICD-10 and DSM-IV at baseline. Depression, anxiety, somatization, eating disorder and posttraumatic stress disorder (PTSD) symptoms, as well as personality functioning were assessed by means of questionnaires on admission and at discharge.Results2,094 patients recruited by 19 participating university hospitals consented to participation in the study. Effect sizes for each of the outcome criteria were calculated for 4–5 sub-groups per outcome domain with differing severity at baseline. Pre-post effect sizes for patients with moderate and high symptom severity at baseline ranged from d = 0.78 to d = 3.61 with symptoms of PTSD, depression, and anxiety showing the largest and somatization as well as personality functioning showing somewhat smaller effects.ConclusionsInpatient and day hospital treatment in German university departments of Psychosomatic Medicine and Psychotherapy is effective under field conditions.Clinical trial registrationhttps://drks.de/search/de/trial/DRKS00016412, identifier: DRKS00016412.