bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adipose stromal cells bioproducts as cell-free therapies: manufacturing and therapeutic dose determine in vitro functionality
Authors: Skovronova Renata , Scaccia Eleonora , Calcat-i-Cervera Sandra , Bussolati Benedetta , O’Brien Timothy , Bieback Karen 
Published: August 2023

Abstract
Background
Extracellular vesicles (EV) are considered a cell-free alternative to mesenchymal stromal cell (MSC) therapy. Numerous reports describe the efficacy of EV in conferring immunomodulation and promoting angiogenesis, yet others report these activities to be conveyed in EV-free bioproducts. We hypothesized that this discrepancy may depend either on the method of isolation or rather the relative impact of the individual bioactive components within the MSC secretome.
Methods
To answer this question, we performed an inter-laboratory study evaluating EV generated from adipose stromal cells (ASC) by either sequential ultracentrifugation (UC) or size-exclusion chromatography (SEC). The effect of both EV preparations on immunomodulation and angiogenesis in vitro was compared to that of the whole secretome and of the EV-free protein fraction after SEC isolation.
Results
In the current study, neither the EV preparations, the secretome or the protein fraction were efficacious in inhibiting mitogen-driven T cell proliferation – highlighting the need for direct cell-to-cell contact. However, EV generated by SEC stimulated macrophage phagocytic activity to a similar extent as the secretome. In turn, tube formation and wound healing were strongly promoted by the ASC secretome and protein fraction, but not by EV. Within the secretome/protein fraction, VEGF was identified as a potential driver of angiogenesis, and was absent in both EV preparations.
Conclusions
Our data indicate that the effects of MSCs on immunomodulation and angiogenesis are EV-independent and rely on direct cell-to-cell contact or on the secretion of soluble factors, respectively. Specific MSC-EV effects need to be dissected for their use as cell-free therapeutics.
Administration of ivermectin to cattle induced mortality, reduced fecundity and survivorship of Anopheles arabiensis in Ethiopia: an implication for expansion of vector toolbox
Authors: Damene Ephrem , Massebo Fekadu 
Published: August 2023

Abstract
Background Although many countries have shown interest in eliminating malaria, approaches that complement existing vector control interventions are needed because existing methods have been scaled up and malaria is still present. Therefore, the effect of ivermectin administration to cattle was evaluated for its effect on mortality, survivorship and mortality of laboratory reared Anopheles arabiensis.
Methods Three calves were randomly selected and injected with ivermectin at a therapeutic dose of 0.2 mg/kg, while the other two calves received no treatment and served as controls. Five tents were constructed for the trial. Calves were housed in tents (one per tent) and then 30 hungry female An. arabiensis were introduced into each tent. Only fully engorged females were collected from each tent and placed in different mosquito cages to monitor their mortality, survival and fecundity. Data analysis was done using SPSS version 16.
Results During the follow-up period (until day 21), ivermectin induced significantly higher mortality when compared to controls. It resulted in an average 24-hour mortality rate of 81.6% against An. arabiensis on the first day following treatment. 100% An. arabiensis that fed on ivermectin-treated calves on the first day after treatment died within four days. Egg production rate of An. arabiensis that fed on ivermectin-treated calves was significantly lower compared to controls (F = 768.7, P < 0.001).
Conclusion In conclusion, ivermectin induced mortality, reduced fecundity and survivorship of laboratory maintained An. arabiensis. Further study is recommended using a wild mosquito population. Moreover, mass ivermectin administration to domestic animals could be recommended to supplement the existing indoor based interventions.
Functional restoration of REM sleep fragmentation, hippocampal oscillatory activity and altered cholinergic signaling at presymptomatic stages of AD
Authors: Berg Monica van den , Heymans Loran , Toen Danielle , Adhikari Mohit A. , Audekerke Johan van , Verschuuren Marlies , Pintelon Isabel , De Vos Winnok H. , Linden Annemie van der , Verhoye Marleen , Keliris Georgios A. 
Published: August 2023

AbstractBackgroundAlzheimer’s disease (AD) is a severe neurodegenerative disorder that is hallmarked by the progressive accumulation of amyloid beta (Aβ) and tau proteins in the brain, leading to dementia. Sleep alterations occur at early stages of AD, before cognitive symptoms become apparent, which could play an important role in the pathophysiology and detection of AD. The current study aimed to evaluate how circadian rhythm, sleep macro-architecture and hippocampal oscillations are altered at pre-plaque and early-plaque stages of AD in TgF344-AD rats.MethodsWe performed 24-hour hippocampal electrophysiological measurements in TgF344-AD rats and wildtype littermates at pre- and early-plaque stages of AD, combined with histological analysis to evaluate histopathological alterations.ResultsWe observed a differential impact of AD on rapid eye movement (REM) and non-REM (NREM) sleep. Circadian rhythmicity was intact and TgF344-AD rats did not show signs of NREM sleep fragmentation. We observed a significantly increased probability for shorter REM bouts, suggestive of REM sleep fragmentation, in TgF344-AD rats at the pre-plaque stage, which recovered at the early-plaque stage. In addition, we observed a significantly decreased theta-gamma coupling, a measure for task-related information ordering, at the pre-plaque stage, which was partially restored at the early-plaque stage. Moreover, theta-gamma coupling in the slow gamma range was significantly increased during the pre-plaque stage in TgF344-AD rats but returned to WT levels at the early-plaque stage. Interestingly, the partial compensation of hippocampal activity and REM sleep behavior coincided with an increased number of cholinergic synapses in the hippocampus during the early-plaque stage in TgF344-AD rats, suggestive of basal forebrain cholinergic compensation mechanisms.ConclusionThe results from this study reveal early changes in sleep architecture and hippocampal function prior to Aß plaque deposition in AD. In addition, the current findings highlight the important role of the cholinergic system to compensate for AD-related network alterations. Network disturbances and sleep alterations are known to drive disease progression. Modulation of cholinergic signaling in early, presymptomatic AD might be a promising therapeutic strategy to alter disease progression by restoring network function and sleep architecture.
Comparative analysis of chloroplast and mitochondrial genomes of sweet potato provides evidence of gene transfer
Authors: li Guoliang , zhang hong , lin zhaomiao , li huawei , xu guochun , xu yongqing , ji rongchang , luo wenbin , qiu yongxiang , qiu sixin , tang hao 
Published: August 2023

Abstract
The increasing number of plant mitochondrial genomes (mtDNA) sequenced reveals the extent of transfer from both chloroplast (cpDNA) and nuclear genomes (nDNA). This study created a library and assembled the chloroplast and mitochondrial genomes of the leafy sweet potato Fucaishu18 better to understand the extent of mitochondrial and chloroplast gene transfer. The full-length chloroplast genome of Fucaishu18 (OM808940) is 161387 bp, with 132 genes annotated, including 87 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. The mitochondrial genome (OM808941) was 269578 bp in length and contained 70 functional genes, including 41 protein-coding genes, 6 rRNA genes, and 24 tRNA genes. 68 SSR loci were found in the leafy sweet potato organelle genome, including 54 in the chloroplast genome and 14 in the mitochondria genome. In the sweet potato mitochondrial genome, most genes have RNA editing sites, and the conversion ratio from hydrophilic amino acids to hydrophobic amino acids is the highest, reaching 47.12%. Horizontal transfer occurs in the sweet potato organelle genome and nuclear genome. 40 mitochondrial genome segments share high homology with 14 chloroplast genome segments, 33 of which may be derived from chloroplast genome horizontal transfer. 171 mitochondrial genome sequences come from the horizontal transfer of nuclear genome.The phylogenetic analysis of organelle genes revealed that the leafy sweet potato was closely related to the tetraploid wild species Ipomoea tabascana and the wild diploid species Ipomoea trifida.
Robust Alternating Multilayered Cellulose Nanofiber Composite Membranes Fabricated by High-Efficiency Ca 2+ -Precomplexed Vacuum-Assisted Self- Assembly towards Electromagnetic Interference Shielding
Authors: Zeng Lingjun , Xue Bai , Wu Changmei , Qi Wenjing , Ai Peng , Xie Lan , Zheng Qiang 
Published: August 2023

Abstract
One-dimensional cellulose nanofiber (CNF) is prone to constructing connected nanofiber networks in vacuum-assisted self-assembly due to intense hydrogen bonds, which exhibits great superiority in fabricating electromagnetic interference (EMI) shielding composite membranes. However, time-consuming vacuum-assisted assembly process creates vast bottlenecks for spreading EMI shielding CNF composites. Herein, a Ca2+-precomplexed vacuum-assisted self-assembly strategy is first proposed to high-efficiently assemble robust CNF&Carbon nanotube/Polyethylene oxide (CNF&CNT/PEO) composite membranes with alternating multilayer architectures. The introduction of Ca2+ pre-complexation can not only largely improve the fabrication efficiency but also immensely enhance the mechanical properties of alternating multilayered CNF&CNT/PEO membranes. The self-assembly time of CNF-5&CNT/PEO-4 is greatly decreased to 756 min at 0.45 mmol/L Ca2+, in comparison with the common vacuum-assisted filtration (i.e., without Ca2+) of 1192 min, owing to the hydrogen bonds between CNF and water broken down by Ca2+ complexation. With the Ca2+ concentration increasing from 0 to 0.23 further to 0.45 mmol/L, the tensile strength of CNF-3&CNT/PEO-2 is gradually reinforced from 40.2 to 43.2 and to 47.6 MPa, as a consequence of the excellent Ca2+ crosslinking and hierarchical “Zigzag” crack paths. The favorable electrical conductivity and unique alternating multilayered structures endow CNF-5&CNT/PEO-4 membrane with the maximal EMI shielding effectiveness (SE) of 43.3 dB. The Ca2+-precomplexed strategy sheds new light on high-efficient self-assembly of multilayered CNF composite membranes with pretty EMI shielding performances, which has prospective application in advanced electronics and microelectronic packaging.
Transcriptional and epigenetic changes during tomato yellow leaf curl virus infection in tomato
Authors: Romero-Rodriguez Beatriz , Petek Marko , Jiao Chen , Križnik Maja , Zagorščak Maja , Fei Zhangjun , Bejarano Eduardo R. , Gruden Kristina , Castillo Araceli G. 
Published: August 2023

Abstract
Background: Geminiviruses are DNA plant viruses that cause highly damaging diseases affecting crops worldwide. During the infection, geminiviruses hijack cellular processes, suppress plant defenses, and cause a massive reprogramming of the infected cells leading to major changes in the whole plant homeostasis. The advances in sequencing technologies allow the simultaneous analysis of multiple aspects of viral infection at a large scale, generating new insights into the molecular mechanisms underlying plant-virus interactions. However, an integrative study of the changes in the host transcriptome, small RNA profile and methylome during a geminivirus infection has not been performed yet. Using a time-scale approach, we aim to decipher the gene regulation in tomato in response to the infection with the geminivirus, tomato yellow curl virus (TYLCV).Results: We showed that tomato undergoes substantial transcriptional and post-transcriptional changes upon TYLCV infection and identified the main altered regulatory pathways. Interestingly, although the principal plant defense-related processes, gene silencing and the immune response were induced, this cannot prevent the establishment of the infection. Moreover, we identified extra- and intracellular immune receptors as targets for the deregulated microRNAs (miRNAs) and established a network for those that also produced phased secondary small interfering RNAs (phasiRNAs). On the other hand, there were no significant genome-wide changes in tomato methylome at 14 days post infection, the time point at which the symptoms were general, and the amount of viral DNA had reached its maximum level, but we were able to identify differentially methylated regions that could be involved in the transcriptional regulation of some of the differentially expressed genes.Conclusion: We have conducted a comprehensive and reliable study on the changes at transcriptional, post-transcriptional and epigenetic levels in tomato throughout TYLCV infection. The generated genomic information is substantial for understanding the genetic, molecular and physiological changes caused by TYLCV infection in tomato.
Unique Measure for Time-Dependent Random PDEs
Authors: Varner Gregory 
Published: August 2023

Abstract
This paper proves the uniqueness of measure for time-inhomogeneous random dynamical systems that exhibit particular controllability and coupling conditions. This is done by extending a result for uniqueness of measure for time-homogeneous Markov processes to the time-inhomogeneous case, showing that the Markov process is exponentially mixing in the dual-Lipschitz norm. It is then shown that the 2D Navier-Stokes equations on the sphere with a time-dependent deterministic force and a "kick"-type random perturbation satisfy the conditions and thus have a unique limiting measure.
MSC Classification: 35Q30 , 60H15 , 60J05 , 93C20 , 35R01 , 60J99
Migration of double J stent from left kidney to Right Vertebral vein, a case report and review of literature
Authors: Hassani Amir Hossein , Kojuri Javad , Bazroodi Helia , Fatemian Hossein , Naderi Nima , Kooshafar Saeed , Dehghani Anahita , Haghpanah Abdolreza 
Published: August 2023

Abstract

Background: In urologic procedures, a double-J stent (DJS) may be employed to facilitate efficient drainage of urine from the kidneys into the bladder. This stent can be inserted either retrogradely or anterogradely. One of the infrequently documented complications of this procedure is the migration of the stent through the renal vein.
Case Presentation: A 69-year-old female with a solitary functioning kidney presented to our clinic with the primary complaint of left flank pain. Subsequent to diagnostic investigations, a sizable mid-pole renal stone was identified. The patient underwent percutaneous nephrolithotomy with the insertion of an anterograde DJS. During her follow-up visit for DJS removal, the stent was not visualized through cystoscopy. Further evaluations revealed the migration of the stent through the renal vein. The distal end of the stent was located within the vertebral vein. However, the patient remained asymptomatic and no thrombosis was found in CT angiography. The stent was then retrieved using an endovascular procedure via the femoral vein.
Conclusion: in this case report, we described a case with asymptomatic DJS migration to the right vertebral vein as the furthest reported location yet.
Mitogenomic characterization of South African leopards (Panthera pardus), and the effect of past climatic events
Authors: Tensen Laura , Emami-Khoyi Arsalan , Camacho Gerrie , Swanepoel Lourens , Fischer Klaus 
Published: August 2023

Abstract

Revealing phylogeographic structure is important for accurate subspecies delineation and understanding a species’ evolutionary history. In leopards (Panthera pardus), there are currently nine subspecies recognized. On the African continent, only one subspecies occurs (P. p. pardus), although historic mitochondrial DNA suggests the presence of three putative continental lineages: (1) West Africa (WA), (2) Central Africa (CA), and (3) Southern Africa (SA). So far, genome-wide data did not recover this phylogeographic structure, although leopards in the southern periphery of their distribution range in Africa have not yet been investigated in detail. The Mpumalanga province of South Africa is of particular interest, as here the CA and the SA clade possibly meet. The aim of this study was to characterize the first mitogenomes of African leopards from Mpumalanga, to help clarifying how South African leopards fit into continental patterns of genetic differentiation. Complete mitogenomes from six leopards were assembled de novo and included in phylogenetic analysis, in combination with other publicly available mitogenomes. Bayesian inference and Maximum Likelihood analyses identified two deeply diverged putative lineages within South Africa, which are more genetically distinct than two subspecies in Asia. The lineages dated back to 0.73–0.87 million years ago, indicating that they originated during the climatically unstable Mid-Pleistocene, as seen in other large mammals. The Pleistocene refuge theory states that the maintenance of savanna refugia in South Africa promoted the divergence between populations. As such, leopards may reflect the unique climatic history of South Africa, which has resulted in eminent and endemic genetic diversity.
Enhanced Antioxidant Properties of Novel Curcumin Derivatives: A Comprehensive DFT Computational Study
Authors: Boulmokh Yamina , Belguidoum Karima , Meddour Faiza , Amira-Guebailia Habiba 
Published: August 2023

Abstract
Oxidative stress, driven by the accumulation of free radicals and reactive oxygen species (ROS) in the human body, is a key contributor to various diseases. Curcumin, a polyphenolic compound derived from turmeric, has garnered attention for its antioxidant potential. In this context, a recent experimental study by Hao et al. introduced curcumin derivatives with incorporated electron-donating groups (allyl and isopentenyl), aiming to enhance antioxidant activity while circumventing the limitations of traditional curcumin. Building upon this experimental foundation, our study employs computational techniques (DFT) to unravel the molecular mechanisms underpinning the superior antioxidant effects observed in these novel derivatives. We investigated three prominent antioxidant mechanisms: Hydrogen Atom Transfer (HAT), Single Electron Transfer-Proton Transfer (SET-PT), and Sequential Proton Loss Electron Transfer (SPLET). Our results reveal that the allyl and isopentenyl groups play pivotal roles in enhancing the antioxidant properties of the derivatives, as evidenced by reduced bond dissociation energies (BDEs) and favorable proton dissociation energies (PDEs). Moreover, the analysis of Highest Occupied Molecular Orbital (HOMO) and Lowest Unoccupied Molecular Orbital (LUMO) energies indicates their enhanced reactivity as electron donors and acceptors. Notably, the computational investigation of spin densities validates the radical scavenging potential of these derivatives. While curcumin exhibits limitations such as low water solubility, bioavailability, and stability, our findings suggest that the strategically designed derivatives (especially exhibit superior antioxidant properties, positioning them as promising candidates for further therapeutic applications. This comprehensive study bridges experimental findings with computational insights to unravel the intricate molecular mechanisms driving the enhanced antioxidant efficacy of the newly developed curcumin derivatives.
Probing the structral, electronic and optical properties of phosphides XmAl 2 S 4 (Xm=Eu,Fe,Rh) compounds for prospective high-energy applications employing the DFT framework
Authors: Irfan Muhammad , Shaheen Nusrat , Saleh Ebraheem , Moharam M. M. , El-Zahhar Adel A. , Asif Sana Ullah , Eldin Sayed M 
Published: August 2023

Abstract

Phosphides XmAl2S4 have received significant attention in the scientific community due to their profound importance in the fields of optoelectronics. In this study, we present a comprehensive Evaluation of the optoelectronic characteristics of XmAl2S4(Xm = Eu,Fe,Rh). Our investigation focuses on both spins up and down of the material, and we use a first-principles density functional theory (DFT) approach to obtain accurate results. This research provides a detailed understanding of the optoelectronic properties of investigated phosphides for the first time. In order to achieve the optoelectronic properties, we employed a precise technique known as full-potential linearized augmented plane wave (FPLAPW) within the frame of the generalised gradient approximation (GGA). This method was calibrated using the Hubbard potential U as exchange-correlation function. The calculated band gaps of phosphides XmAl2S4(Xm = Eu,Fe,Rh) were approximately 4.2 eV/ 3.4 eV, 1.3 eV/ 2.4 eV and 3.0 eV/ 2.1 eV (Up/Dn spins), respectively. The phase stability in these phosphides was verified by calculating their negative formation energy values. This analysis included the determination of the extinction coefficient, absorption coefficient, energy loss function, reflectivity, refractive index, and real optical conductivity. The optical parameters determined in both phases exhibited a significant spin-dependent phenomenon. The phosphor FeAl2S4 and FeAl2S4 displays significantly higher green color purity. Therefore, exhibits potential as a green phosphor candidates in the field of solid-state lighting LED technology and optoelectronic industory.
Causal effects of Sjögren's syndrome on cancer risk: A Mendelian Randomization Study
Authors: Cheng Hao , Xu Jin-Hong , Wu Chen-Chen , Wang Zhi-Xia , Xu Xue-Lian 
Published: August 2023

Abstract
Objective Previous studies have reported the association between sjögren's syndrome (SS) and some types of tumors. However, the causal associations between SS and different types of cancer remain inconclusive. This research aimed to assess the causal relationships between SS and cancer risk by the mendelian randomization (MR) analysis.Methods Two-sample MR analysis was performed by using the genome-wide association studies (GWAS) data and the FinnGen data. Qualified instrumental variables (IVs) were singled out based on the assumptions of MR analysis. The main method was the inverse-variance weighted (IVW). Sensitivity tests were also applied.Results Genetically determined SS has a causal effect on an increased risk of marginal zone lymphoma (IVW odds ratio (OR) = 1.9425, 95% confidence interval (CI): 1.5229–2.4776, P = 8.9E-08). Moreover, there was no causal effect between SS and other cancer risks in the MR analysis (IVW P > 0.05).Conclusions Our results investigated that genetically determined SS increases the risk of marginal zone lymphoma. During the treatment of SS, we should pay more attention on whether to appear the clinical symptoms of marginal zone lymphoma.