bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vectorizing and distributing number‐theoretic transform to count Goldbach partitions on Arm‐based supercomputers
Authors: Jesus Ricardo , Oliveira e Silva Tomás , Weiland Michèle 
Published: August 2023

SummaryIn this article, we explore the usage of scalable vector extension (SVE) to vectorize number‐theoretic transforms (NTTs). In particular, we show that 64‐bit modular arithmetic operations, including modular multiplication, can be efficiently implemented with SVE instructions. The vectorization of NTT loops and kernels involving 64‐bit modular operations was not possible in previous Arm‐based single instruction multiple data architectures since these architectures lacked crucial instructions to efficiently implement modular multiplication. We test and evaluate our SVE implementation on the A64FX processor in an HPE Apollo 80 system. Furthermore, we implement a distributed NTT for the computation of large‐scale exact integer convolutions. We evaluate this transform on HPE Apollo 70, Cray XC50, HPE Apollo 80, and HPE Cray EX systems, where we demonstrate good scalability to thousands of cores. Finally, we describe how these methods can be utilized to count the number of Goldbach partitions of all even numbers to large limits. We present some preliminary results concerning this problem, in particular a histogram of the number of Goldbach partitions of the even numbers up to 240.
Impact of a corporate social responsibility message on consumers' sustainable behaviours and purchase intentions
Authors: Louis Didier , Lombart Cindy 
Published: August 2023

AbstractThis study investigates the impact of a water brand's CSR message highlighting the brand's environmental concerns on brand‐related variables (attitude towards the brand and CSR) and consumer‐related variables (consumer efforts to adopt sustainable behaviours and well‐being). A research model is proposed, and the relationships postulated are tested on 414 French consumers. The study establishes the effectiveness of this kind of CSR messaging in influencing French consumers to purchase and recommend a particular brand. It then shows the direct and indirect ways in which a brand's CSR messaging can improve consumers' behavioural intentions (i.e., intentions to purchase the brand's products and to recommend the brand and/or its products). It also underscores that brand‐related variables contribute to increasing brands', and thus companies', business performance, whereas consumer‐related variables contribute to increasing their social performance. Finally, the REBUS‐PLS method emphasises the existence of several consumer groups and identifies the core target customer groups on which companies should focus their communication efforts.
Water temperature and chlorophyll a density drive the genetic and epigenetic variation of Vallisneria natans across a subtropical freshwater lake
Authors: Li Yixian , Xia Manli , Zhao Xuyao , Hou Hongwei 
Published: August 2023

AbstractPlant genetic diversity differs in habitat's oscillations, especially species distributed under heterogeneous environmental conditions. Freshwater ecosystems are vulnerable to biotic and abiotic impacts, which affect the genetic and epigenetic variations in aquatic plants. The extent of environmental heterogeneous attributes can be examined based on genetic and epigenetic variations. Such variations under environmental gradient can provide evidence for understanding the correlations between rapid environmental changes and species evolution. In this study, we performed amplified fragment polymorphism length and methylated‐sensitive amplified polymorphism analysis to depict the genetic and epigenetic variations of Vallisneria natans in a subtropical lake. Results showed that this species maintained a relatively high genetic diversity (mean HE = 0.320, I = 0.474, PPL = 85.93%) and epigenetic variation (mean eHE = 0.282, eI = 0.428, ePPL = 83.91%). Water body temperature and chlorophyll a density were positively correlated to the genetic and epigenetic variations. The clonal generates of V. natans depicted a relative high methylation level and shew ancestral scenario between the genet and the second clonal generation. These findings revealed that species diversity is unevenly distributed under environmental heterogeneity, even at a fine geographic scale. Environmental characteristics in relation to temperature and chlorophyll a should be considered in the analysis of the genetic and epigenetic variations. Additionally, epigenetic variations between genets and ramets should be considered with caution when applied to analysis of other aquatic species.
Removal ofN‐fixing vs.non‐N‐fixing herbs in postfire chaparral: Competition and contributions to soil N and C cycling
Authors: Hendricks‐Franco Lindsey , Stephens Scott L. , Silver Whendee L. , Sousa Wayne P. 
Published: August 2023

AbstractAs climate change increases fire frequency in Mediterranean‐type shrublands, it is essential to understand the links between common postfire plant assemblages and soil nitrogen (N) and carbon (C) cycling during succession. In California chaparral, periodic fire removes shrub cover, deposits ammonium (NH4+‐N) on soils, and allows herbaceous assemblages to dominate for 3–5 years. Herbs influence soil biogeochemistry through several mechanisms, including nutrient uptake, litter decomposition, and rhizodeposition. Controlled experimental removal of select plant groups from wild assemblages can demonstrate interactions between plant groups and how plant traits influence belowground processes. In a two‐year herb‐removal experiment, we investigated the impact of N‐fixing and non‐N‐fixing herbs on soil N and C cycling. Treatments were (1) all herbs, (2) only non‐N‐fixing species, (3) only N‐fixing species, and (4) no herbs. In high‐N environments, N‐fixers were predicted to compete poorly against non‐N‐fixing neighbors. N‐fixers doubled in abundance when non‐N‐fixers were removed, but non‐N‐fixers were unaffected by N‐fixer removal. Two years after fire, no‐herbs plots had the lowest soil microbial respiration rates, and total accumulated C and N were lower than all‐herb plots. Two treatments, no‐herb and N‐fixer plots, had elevated mineral N concentrations, net N mineralization, and net nitrification in the second year of the experiment. Our findings underscore the importance of fire‐following herbs for postfire N retention and organic matter accumulation. A combination of both N‐fixing and non‐N‐fixing herbs maximized total soil C and N, although the accumulation of TC and TN in all‐herb plots was not significantly higher than in non‐N‐fixer plots. Results demonstrated the key role of non‐N‐fixing herbs in accumulating soil C and herbaceous communities for retaining N. Elevated soil nutrient availability two years postfire may contribute to the long‐term recovery of shrubs, even after herbs are no longer dominant. Future investigations should also consider the magnitude of soil microbial N retention in plots with different herb functional groups, along with the species‐specific contribution of non‐N‐fixing herbs to postfire C and N cycling.
Molybdenum Tricarbonyl Complexes Supported by Linear PNP Ligands: Influence of P‐ and N‐Substituents on Relative Stability, Stereoisomerism and on the Activation of Small Molecules
Authors: Froitzheim Sven , Junge Jannik , Barnehl Christopher , Engesser Tobias A. , Krahmer Jan , Näther Christian , Tuczek Felix 
Published: August 2023

AbstractSeries of linear tridentate PNPhPR‐ligands (R=Me, Et, Pln, Ph, Cyp, iPr, Cy, tBu) and molybdenum tricarbonyl complexes [Mo(CO)3PNPhPR] (R=Ph, Et, Cyp, iPr, Cy,) were synthesized and characterized using NMR‐, IR‐, and Raman spectroscopy as well as X‐ray crystallography. The influence of the different phosphine donor groups of the PNPhPR ligands on the bonding and activation of CO ligands is investigated. Importantly, all complexes are found to adopt a fac geometry, both in solution and in the solid state. This is in contrast to analogous complexes supported by PNHP ligands. DFT calculations reveal that the phenyl ring at the central amine function is the cause of the preferred geometry, hindering isomerization to a mer geometry.
Novel technique for cognitive magnetic resonance imaging‐transperineal ultrasound fusion transperineal prostate biopsy with injection of bubbled jelly into urethra after abdominoperineal excision
Authors: Miyajima Keiichiro , Urabe Fumihiko , Nakano Juria , Iwatani Kosuke , Tashiro Kojiro , Tsuzuki Shunsuke , Sato Shun , Takahashi Hiroyuki , Kimura Takahiro , Miki Kenta 
Published: August 2023

IntroductionProstate biopsy is commonly performed using a transrectal ultrasound probe through a transrectal or transperineal approach. However, this is not possible for patients without a rectum.Case presentationA 75‐year‐old male was referred to our hospital because of an elevated prostate‐specific antigen and a suspicious prostate lesion (PIRADS 5) in the left peripheral zone. The patient had previously undergone abdominoperineal resection for rectal cancer, which excluded the use of transrectal ultrasound. We describe the use of the transperineal ultrasound‐guided biopsy with cognitive magnetic resonance imaging‐transperineal ultrasound fusion and the utility of the injection of bubbled lidocaine jelly into urethra to improve its visualization. The pathological findings revealed clinically significant cancer with a Gleason score of 5 + 4.ConclusionCognitive magnetic resonance imaging‐transperineal ultrasound fusion transperineal prostate biopsy with injection of bubbled jelly into urethra is a feasible and practical technique that does not require any specialized equipment.
Bioactive chemical constituents from Hyptis suaveolens stem
Authors: Hsu Fu‐Chun , Lin Yi Li , Tsai Sheng Fa , Hsieh Tsung‐Hsin , Lee Shoei‐Sheng 
Published: August 2023

AbstractOur previous studies have found that the aerial part of Hyptis rhomboides and the seed of Hyptis suaveolens contain xanthine oxidase (XO) inhibitors, inspiring us to investigate the chemical constituents of H. suaveolens stem. The EtOH extract of H. suaveolens stem was fractionated by liquid‐liquid partitioning, followed by separation over Sephadex LH‐20, silica gel, and reverse‐phase column chromatography, centrifugal partition chromatography, and semi‐preparative RP‐HPLC, to give 17 bioactive isolates. Of these, dimethyl melitrate A (9) is a new natural product, 10 is likely (7″ E)‐9′‐methyl melitrate A, only caffeic acid (2) is the same as those isolated from the seed of the same plant, and only nepetoidins A (14) and B (11) are the same as those from H. rhomboides stem. Nepetoidin B (11) showed the best anti‐XO activity. Rosmarinic acid (3) is the most abundant (>661 ppm; w/w, dried stem), while melitric acid (12) (>277 ppm), caffeic acid (2; >125 ppm), and methyl rosmarinate (4; >81 ppm) are the major ones. This outcome could serve as a good basis for chemotaxonomy of the Hyptis plants and is beneficial for developing H. suaveolens stem as anti‐hyperuricemic nutraceutical.
Development of oncolytic and gene therapy vectors based on adenovirus serotype 4 as an alternative to adenovirus serotype 5
Authors: Sallard Erwan , Schulte Lukas , van den Boom Alexander , Klimovitskii Alexander , Knierer Julius , Hagedorn Claudia , Knocks Maximilian , Zhang Wenli , Kreppel Florian , Ehrhardt Anja , Ehrke‐Schulz Eric 
Published: August 2023

AbstractBackgroundAdenoviral vectors are among the most frequently used vectors for gene therapy and cancer treatment. Most vectors are derived from human adenovirus (Ad) serotype 5 despite limited applicability caused by pre‐existing immunity and unfavorable liver tropism, whereas the other more than 100 known human serotypes remain largely unused. Here, we screened a library of human Ad types and identified Ad4 as a promising candidate vector.MethodsReporter‐gene‐expressing viruses representative of the natural human Ad diversity were used to transduce an array of muscle cell lines and two‐ or three‐dimensional tumor cultures. The time‐course of transgene expression was monitored by fluorescence or luminescence measurements. To generate replication‐deficient Ad4 vector genomes, successive homologous recombination was applied.ResultsAd4, 17 and 50 transduced human cardiomyocytes more efficiently than Ad5, whereas Ad37 was found to be superior in rhabdomyocytes. Despite its moderate transduction efficiency, Ad4 showed efficient and long‐lasting gene expression in papillomavirus (HPV) positive tumor organoids. Therefore, we aimed to harness the potential of Ad4 for improved muscle transduction or oncolytic virotherapy of HPV‐positive tumors. We deleted the E1 and E3 transcription units to produce first generation Ad vectors for gene therapy. The E1‐ and E1/E3‐deleted vectors were replication‐competent in HEK293 cells stably expressing E1 but not in the other cell lines tested. Furthermore, we show that the Ad5 E1 transcription unit can complement the replication of E1‐deleted Ad4 vectors.ConclusionsOur Ad4‐based gene therapy vector platform contributes to the development of improved Ad vectors based on non‐canonical serotypes for a broad range of applications.
New molecular insights into ferroptosis in lung adenocarcinoma progression and pharmacological compounds for targeted therapy
Authors: Tian Wenhui , Wan Xiaoqing , Tian Lili , Wu Yajun , Cui Xiaohua , Yi Jingyu 
Published: August 2023

AbstractBackgroundThe involvement of ferroptosis has been found in many pathological conditions of the lung. The genetic engineering of ferroptosis‐related genes may provide a potential target for the treatment of lung adenocarcinoma (LUAD).MethodsNine ferroptosis regulators and markers were collected from FerrDb and their somatic mutations and expressions were analyzed based on The Cancer Genome Atlas (TCGA)‐LUAD cohort data. Least absolute shrinkage and selection operator (LASSO) and Cox regression analysis were performed to screen genes significantly associated with ferroptosis. The ferroptosis‐related gene signature was constructed using TCGA‐LUAD cohort data and was verified using the GSE cohort with pooled data for GSE30219, GSE31210, GSE37745 and GSE50081. Immune microenvironment component and mutation analysis were performed for genes in the ferroptosis‐related gene signature.ResultsAll nine ferroptosis regulators and markers were differentially expressed between normal LUAD tumor tissues and adjacent normal tissues and were related to copy number variation. The expression of 1329 genes were significantly associated with nine ferroptosis regulators and markers in the TCGA‐LUAD dataset, five (ALDOA, PLK1, CD47, CENPC and TMOD3) of which were integrated into a ferroptosis‐related gene signature to calculate the risk score of LUAD samples, showing a significant correlation with the abundance of immune cell infiltration and the immune score. Molecular docking showed the binding activity of natural active compound quercetin to target proteins ALDOA and CD47, as well as the binding activity of aristolochic acid to PLK1 protein and TMOD3 protein.ConclusionsIn the present study, a ferroptosis‐related gene signature with predictive value for LUAD prognosis was constructed, in which the gene was a potential therapeutic target for LUAD. Quercetin and aristolochic acid were potential candidates for inhibiting these targets by directly binding to them and showing high affinity and strong stability.
Multi‐omics analysis reveals that ferroptosis‐related gene CISD2 is a prognostic biomarker of head and neck squamous cell carcinoma
Authors: Li Zhengrui , Wang Qi , Huang Xufeng , Fu Rao , Wen Xutao , Zhang Ling 
Published: August 2023

AbstractBackgroundHead and neck squamous cell carcinoma (HNSCC) is a prevalent malignancy, with high mortality rate and unavailability of accurate therapies. However, its early prevention remains a challenge. In the purview of predictive, preventive, and personalized medicine (PPPM), it is paramount to identify novel and powerful biomarkers. CISD2 is a crucial regulator of iron homeostasis and reactive oxygen species (ROS). Recent studies showed that the NEET protein (NAF‐1) encoded by CISD2 is involved in regulating the proliferation and metastasis of tumor cells. Nevertheless, the prognostic value and immunological correlations of CISD2 remain unclear.MethodsBioinformatics analyses conducted utilizing data from comprehensive databases The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). All statistical evaluations were executed employing R software.ResultsOur investigation of biological function, enrichment pathway, and immune correlation revealed a discernable linkage between CISD2 and the immune response. Moreover, we found that the suppression of CISD2 is associated with immune cell infiltration and various immune signatures.ConclusionsThe present study successfully revealed the potential prognostic and biological function of CISD2 in HNSCC. High expression of CISD2 are linked to gender, race, grade, etc., can notably enhance the early detection, prognosis, and prediction for individuals afflicted with HNSCC.
Atmospheric aerosol particles before, during, and after a convective dust event on the Jornada experimental range as measured using Raman hyperspectral imaging
Authors: Doughty David C. , Dumais Robert E. , Richman Tyler R. , Hill Steven C. 
Published: August 2023

AbstractThe effects of winds and local mineralogy on ambient aerosol composition are poorly understood. We measured the Raman spectra (RS) of ambient aerosol particles on the Jornada Experimental Range, a desert location in southern New Mexico, used these RS to group spectra by composition or spectral features, and compared the numbers of RS with the winds and minerology. An aerosol Raman hyperspectral imager was used to collect particles onto a tape and measure a hyperspectral Raman image in 15‐ or 20‐min intervals. Over a 48 h period, 6306 RS were above the thresholds used and were analyzed further. Multiple RS may originate from the same particle. Of these 6306 RS, 2567 were classified as luminescence; 2647 contained the D and G peaks of DG Carbon (DGC, which includes soots, black carbon, and similar materials); 43 exhibited peaks consistent with CH stretching; and 130, 102, and 29 RS were consistent with quartz, carbonates (calcite and dolomite), and potassium feldspar, respectively. A convective dust event was concurrent with an increase in the number of RS of luminescent particles >20X the median; numbers of DGC RS in the interquartile range of DGC for the entire measurement period; RS consistent with quartz, calcite, iron oxides, feldspar, and anatase; and no RS consistent with oxalates or nitrates. This work shows that hyperspectral Raman imaging can help understand the time‐dependent composition of ambient aerosol particles at time resolutions below an hour.
Vibrational spectroscopic, crystallographic, and microscopic investigation on the Aioun El Atrouss diogenite
Authors: Ayvaz Irmak , Unsalan Ozan , Castro Kepa , Altunayar‐Unsalan Cisem , Zolensky Michael Ewing 
Published: August 2023

AbstractDiogenites are thought to have originated from asteroid 4 Vesta asteroid, and it has been previously shown that they mostly consist of orthopyroxene, chromite, and olivine minerals. In this study, the Aioun El Atrouss (AAT‐1) diogenite was analyzed by vibrational spectroscopic (FT‐IR and micro‐Raman), EDXRF, and XRD techniques. AAT‐001 is mainly composed of pyroxene and with lesser olivine. XRD investigation confirmed magnetite, kamacite, ilmenite, and albite. A troilite‐orthopyroxene intergrowth was revealed by XRF. The olivine composition was estimated to be approximately Fo66 by Raman olivine characteristic doublets. Pyroxenes in AAT‐01 were found to be En80Fs20. Finally, approximate peak shock pressure of more than 86 GPa for AAT‐1 was suggested based on previous hypervelocity impact experiments on olivine.
Amelioration of saline‐alkali land by cultivating Melia azedarach and characterization of underlying mechanisms via metabolome analysis
Authors: Li Na , Shao Tianyun , Jia Bingzhi , Yan Xiao , Wang Xinglan , Tao Chenyunzhu , Long Xiaohua , Liu Manqiang , Zhou Zhaosheng , Rengel Zed 
Published: August 2023

AbstractSoil salinization is a serious problem leading to ecological degradation. Melia azedarach is highly salt‐tolerant, and its application to saline‐alkali land is a promising strategy for restoring degraded lands. However, most studies only focus on the salt‐reducing effect of herbs on saline‐alkali land, and there are few studies on the metabolic adaptation mechanism of trees in the process of soil restoration in coastal saline‐alkali land. In this study, we analyzed the soil properties and metabolome of M. azedarach roots grown in low‐ (<3 g/kg; L), medium‐ (5 ~ 8 g/kg; M), and high‐ (>10 g/kg; H) salinity soils to explore the amelioration effect and adaptation mechanism of M. azedarach to soils with differential salinity. Cultivation of M. azedarach was associated with a decrease in the concentration of Na+ and increases in organic matter content and alkaline phosphatase and urease activities in the rhizosphere soil. The metabolome analysis revealed that a total of 382 (ESI+) and 277 (ESI−) differential metabolites (DEMs) were detected. The number of DEMs in roots rose with increased soil salinity, such as sugars and flavonoids in H versus L, and amino acids in M versus L. The most up‐regulated DEMs were 13‐S‐hydroxyoctadecadienoic acid, 2′‐Deoxyuridine, and 20‐hydroxyleukotriene B4. Combined analysis of soil properties and M. azedarach DEMs indicated that alkaline phosphatase activity was positively correlated with traumatic acid concentration. Taken together, these results indicate that M. azedarach has the potential to reduce soil salinity and enhance soil enzyme activity, and it can adapt to salt stress by regulating metabolites like sugars, amino acids, and flavonoids. This study provides a basis for understanding the mechanism underlying the adaptation of M. azedarach to saline‐alkali soil and its amelioration.
Straightforward Cell Patterning with Ultra‐Low Background Using Polydimethylsiloxane Through‐Hole Membranes
Authors: Zhou Yujie , Sun Meilin , Xuanyuan Tingting , Zhang Jinwei , Liu Xufang , Liu Wenming 
Published: August 2023

AbstractMicropatterning is becoming an increasingly popular tool to realize microscale cell positioning and decipher cell activities and functions under specific microenvironments. However, a facile methodology for building a highly precise cell pattern still remains challenging. In this study, A simple and straightforward method for stable and efficient cell patterning with ultra‐low background using polydimethylsiloxane through‐hole membranes is developed. The patterning process is conveniently on the basis of membrane peeling and routine pipetting. Cell patterning in high quality involving over 97% patterning coincidence and zero residue on the background is achieved. The high repeatability and stability of the established method for multiple types of cell arrangements with different spatial profiles is demonstrated. The customizable cell patterning with ultra‐low background and high diversity is confirmed to be quite feasible and reliable. Furthermore, the applicability of the patterning method for investigating the fundamental cell activities is also verified experimentally. The authors believe this microengineering advancement has valuable applications in many microscale cell manipulation‐associated research fields including cell biology, cell engineering, cell imaging, and cell sensing.