bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcutaneous electrical acupoint stimulation to reduce opioid consumption in patients undergoing inguinal hernia repair: protocol for a randomized controlled trial
Authors: Agrawal Siddarth , Szmit Mateusz , Wełna Marek , Rudnicki Jerzy , Agrawal Anil , Goździk Waldemar 
Published: December 2022

Abstract
Background
The purpose of this study is to evaluate the efficacy and safety of transcutaneous electrical acupoint stimulation (TEAS) in the postoperative treatment of patients undergoing inguinal hernia repair compared with sham and no treatment group.

Methods
This study is a protocol for a three-armed, single-blinded, placebo-controlled randomized controlled trial. Ninety participants scheduled for inguinal hernia repair will be randomly assigned to the TEAS group (n = 30), sham group (n = 30), and control group (n = 30). The TEAS group will receive treatment using four portable coin-sized electro-stimulators at both local and distal acupuncture points. The sham group will receive sham treatment with mock electrostimulation. The treatment groups will receive mixed frequency stimulation (alternating at 2 and 100 Hz every 3 s) in continuous mode for 30 min at intervals of 2 h for 24 h postoperatively. The control group will receive postoperative pain control using patient-controlled analgesia (PCA) device. The primary outcome is the total morphine dose received in the postoperative period (mg) using PCA 24 h after surgery. The number of PCA demands (i.e., times the button will be pressed) and delivered bolus doses, score on the Visual Analogue Scale, opioid-related side effects, the requirement for supplemental medications, score on the Hospital Anxiety and Depression Scale (HADS), and blood levels of stress hormones cortisol and prolactin.

Discussion
The results of this trial will determine whether TEAS with intensified stimulation protocol is a safe and effective option for reducing analgesic consumption and postoperative pain.

Trial registration
ISRCTN76428396. Registered on 05 October 2020. https://www.isrctn.com/ISRCTN76428396
Tailor-made alkaliphilic and thermostable fungal laccases for industrial wood processing
Authors: Rodríguez-Escribano David , Pliego-Magán Rocío , de Salas Felipe , Aza Pablo , Gentili Patrizia , Ihalainen Petri , Levée Thomas , Meyer Valérie , Petit-Conil Michel , Tapin-Lingua Sandra , Lecourt Michael , Camarero Susana 
Published: December 2022

Abstract
Background
During the kraft process to obtain cellulosic pulp from wood, most of the lignin is removed by high-temperature alkaline cooking, released in the black liquors and usually incinerated for energy. However, kraft lignins are a valuable source of phenolic compounds that can be valorized in new bio-based products. The aim of this work is to develop laccases capable of working under the extreme conditions of high temperature and pH, typical of the industrial conversion of wood into kraft pulp and fibreboard, in order to provide extremophilic biocatalysts for depolymerising kraft lignin, and enzyme-assisted technologies for kraft pulp and fibreboard production.

Results
Through systematic enzyme engineering, combining enzyme-directed evolution and rational design, we changed the optimal pH of the laccase for oxidation of lignin phenols from acidic to basic, enhanced the catalytic activity at alkaline pH and increased the thermal tolerance of the enzyme by accumulating up to eight mutations in the protein sequence. The extremophilic laccase variants show maximum activity at 70 °C and oxidize kraft lignin at pH 10. Their integration into industrial-type processes saves energy and chemicals. As a pre-bleaching stage, the enzymes promote kraft pulp bleachability and significantly reduce the need for chlorine dioxide compared to the industrial sequence. Their application in wood chips during fibreboard production, facilitates the defibering stage, with less energy required.

Conclusions
A set of new alkaliphilic and thermophilic fungal laccases has been developed to operate under the extreme conditions of high temperature and pH typical of industrial wood conversion processes. For the first time basidiomycete laccases of high-redox potential show activity on lignin-derived phenols and polymeric lignin at pH 10. Considering the extreme conditions of current industrial processes for kraft pulp and fibreboard production, the new tailor-made laccases constitute a step forward towards turning kraft pulp mills into biorefineries. Their use as biocatalysts in the wood conversion sector is expected to support the development of more environmentally sound and efficient processes, and more sustainable products.
Precision and geographical prevalence mapping of schistosomiasis and soil-transmitted helminthiasis among school-aged children in selected districts of north-western Tanzania
Authors: Mazigo Humphrey D. , Zinga Maria M. , Kepha Stella , Yard Elodie , McRee-Mckee Kevin , Kabona George , Ngoma Deogratias D. , Nshala Andreas 
Published: December 2022

Abstract
Background
The identification and mapping of at-risk populations at a lower administrative level than the district are prerequisites for the planning, resource allocation and design of impactful control intervention measures. Thus, the objective of the current study was to conduct sub-district precision mapping of soil-transmitted helminthiasis (STH) and schistosomiasis in 29 districts of north-western Tanzania using the current recommended World Health Organization criteria.

Methods
A cross-sectional survey was conducted in 145 schools between March and May 2021. A urine filtration technique was used for the quantification of Schistosoma haematobium eggs, whereas quantification of Schistosoma mansoni and STH eggs was done using the Kato–Katz technique. Microhaematuria was examined using a urine dipstick.

Results
The overall prevalences of any STH and schistosome infections were 9.3% [95% confidence interval (95%CI) 8.6–9.9] and 14.6% (95%CI 13.9–15.4), respectively. The overall prevalence of S. mansoni was 8.7% (95%CI 8.1–9.3), and 36.4%, 41.6%, and 21.9% of the children had low, moderate, and heavy infections, respectively. The overall prevalence of S. haematobium was 6.1% (95%CI 5.5–6.5), and 71.7% and 28.3% of the infected children had light and heavy intensity infections, respectively. The prevalence of microhaematuria was 7.3% (95%CI 6.7–7.8), with males having the highest prevalence (8.4%, P < 0.001). The prevalences of Trichuris trichiura, Ascaris lumbricoides and hookworm were, respectively, 1.3% (95%CI 0.1–1.5), 2.9% (95%CI 2.5–3.3) and 6.2% (95%CI 5.7–6.7). Most of the children infected with STH had light to moderate intensities of infection. The overall prevalence of co-infection with STH and schistosomiasis was 19.1%. The prevalence of schistosomiasis (P < 00.1) and STH (P < 0.001) varied significantly between schools and sub-districts. Schistosoma mansoni and S. haematobium were observed in 60 and 71 schools, respectively, whereas any STH was observed in 49 schools. In schools where schistosomiasis was observed, prevalence was < 10% in 90.8% of them, and ranged from ≥ 10% to < 50% in the other 9.2%. In schools where any STH was observed, the prevalence was < 10% in 87.7% of them.

Conclusions
The data reported here show that schistosomiasis and STH are widely distributed around Lake Victoria. In most of the schools where schistosomiasis and STH occurred the transmission thresholds were low. These data are important and need to be taken into consideration when decisions are made on the implementation of the next round of mass chemotherapies for schistosomiasis and STH in Tanzania.

Graphical Abstract
Entrepreneurship goal and implementation intentions formation: the role of higher education institutions and contexts
Authors: Ndofirepi Takawira Munyaradzi 
Published: December 2022

AbstractThe concept of intentionality is widely recognised for its contribution to understanding pre-planned human behaviour, hence the need to fathom its underlying determinants as a precursor to any attempts to predict or influence future human activity. The purpose of this study was to assess the effect of supportive environments and higher education institutions on students’ entrepreneurial goal and implementation intentions in Zimbabwe. Also, the study sought to evaluate the applicability of Ajzen’s Theory of Planned Behaviour to the context. A cross-sectional quantitative survey of a convinience sample of students from selected higher education institutions in the country was conducted. The 284 responses obtained from the survey were analysed using the partial least squares structural equation modelling statistical technique. The computer software Smart PLS 3 was used for this purpose. The results indicate a direct influence of university support on entrepreneurial goal intention and perceived behavioural control. In addition, they confirm a direct influence of supportive environments on perceived behavioural control and subjective norms. Furthermore, the results demonstrate that perceived behavioural and subjective norms directly influenced entrepreneurial goal intention. Finally, the results show that entrepreneurial goal intention was a significant predictor of entrepreneurial implementation intention. All other tested relationships were not statistically significant. The study makes two significant contributions to the literature. Firstly, it theorises and empirically tests the influence of supportive educational institutions and external environments on separate classes of entrepreneurial intentions within a single study. Secondly, it tests the relevance of the Theory of Planned Behaviour to two distinct but related categories of entrepreneurial intentions.
Transcortical sensory aphasia heralding SARS-Cov-2-induced autoimmune encephalitis with gyral restricted diffusion hyperintensities: a novel case report
Authors: Chakraborty Arka Prava , Pandit Alak , Dutta Ajitava , Das Shambaditya , Ganguly Goutam , Dubey Souvik 
Published: December 2022

Abstract
Background
Coronavirus disease 2019 (CoVID-19), primarily thought of as a respiratory system disease is actually a multi-system disease with immunological implications. CNS involvement in COVID has been explained in recent literature mainly for stroke, encephalopathy, encephalitis, acute disseminated encephalomyelitis and myelopathy. There are few studies characterizing clinical spectrum of COVID autoimmune encephalitis. We present a unique case of post-COVID autoimmune encephalitis in a diabetic male presenting with language dysfunction and novel radiologic findings.

Case presentation
Patient admitted to inpatient department of a tertiary care hospital of India was evaluated by bedside clinical examination, routine blood tests, CSF study with intrathecal SARS-Cov-2 antibody detection, commercially available tests for autoimmune encephalitis, neuroviral panel with HSV PCR, EEG, 3-Tesla MRI and PET scan. Patient was found to have personality change and transcortical sensory aphasia in the outset of COVID encephalitis. MRI findings like temporal involvement and insular ribboning are also being reported. The patient was treated with IV immunoglobulin and is on an improving course.

Conclusions
This case reports dysphasia due to COVID-mediated injury to the language networks, with novel radiologic findings. Role of parainfectious versus immune etiology is also discussed. Further studies are needed to elucidate the mechanism and clinical spectrum of post-COVID autoimmune encephalitis.
Allergic rhinitis - a review on recent advances in characteristics, diagnostic and treatments
Authors: Lisowska Aleksandra , Żaba Ziemwowit , Żołyniak Wojciech , Warzyszak Paweł , Milczek Maria , Małek Róża , Hawranik Izabela , Tomasik Mikołaj , Niski Szymon , Skrzypek Mateusz 
Published: December 2022

Introduction:
Allergic rhinitis is an inflammation of the nasal mucosa characterized by nasal discharge or down the back of the throat, sneezing, stuffy or itchy nose. Allergic rhinitis can be caused by various allergens - pollen, house dust mites, animal allergens, fungi. It can occur periodically or chronically, as a year-round allergic rhinitis. Allergic rhinitis (AR) is a common condition, epidemiological studies suggest that it affects 20–30% of adults and up to 40% of children.

Objective:
The purpose of this review is to analyze the latest information on the characteristics, diagnosis and treatment of patients with allergic rhinitis.

Material and metods:
This review was based on available data collected in the PubMed data base. The research was done by looking through key words such as: “allergic rhinitis”, “Local nasal allergy”, “diagnosis” and “treatment”.

State of knowledge:
The main challenges with allergic rhinitis relate to its treatment. Symptomatic and anti-inflammatory drugs are often used and are not fully effective. The diagnosis of allergic rhinitis can be diagnosed by history and physical examination, with testing reserved for treatment of nonresponders.

Summary:
Treatment should be comprehensive and take into account the environment, pharmacotherapy, allergen immunotherapy, if indicated. As a disease that affects many people and significantly reduces the quality of life, allergic rhinitis deserves a thorough understanding of its symptoms, diagnosis and treatment.
DEVELOPMENT OF A METHOD FOR THE QUANTITATIVE DETERMINATION OF N-DEZACETYLAPPACONITINE HYDROBROMIDE IN THE DRUG OF THE ANTIARRHYTHMINE BY THE METHOD OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ITS CHROMATOGRAPHIC BEHAVIOR
Authors: Mutalova Dilnoza Karimberdievna , Otaeva Shahzoda Akhtamovna , Botirov Ruzali Anvarovich , Sadikov Alimjan Zairovich , Sagdullayev Shamansur Shakhsaidovich 
Published: December 2022

At present, the chemical compositions of plant raw materials for the content of diterpenoid alkaloids have been studied in world research centers in various countries, their pharmacological and toxicological properties have been determined, and technological studies have been carried out in the field of obtaining them in production. At the Institute of Chemistry of Plant Substances, research on the study of diterpenoid alkaloids of plants of the genus Aconite, in particular, research to establish their chemical structure, biosynthesis, pharmacological and toxicological properties of diterpenoid alkaloids, and their production technology.
Increasing requirements for the safety, efficacy, and quality of medicines necessitate the development of new and improvement of existing methods for their analysis. As a rule, the quality of substances is ensured by a set of analytical methods that allow confirming their authenticity, and determining the purity and quantitative content of the active substance. The methods and techniques used for this need to be constantly improved. Considering the above, the problem of the comparative study of physicochemical methods used for the analysis of substances is topical. The object of the study was the substance Antiarrhythmin. A new method for the qualitative and quantitative determination of N-deacetyllappaconitine hydrobromide in Antiarrhythmin substances has been developed. A comparative analysis of the spectrophotometers and the HPLC method was carried out.
The HPLC method in isocratic mode is used for the sensitive determination of the quantitative analysis of Antiarrhythmin. This technique is simple and well reproduced. The validation results showed that the method has good linearity and within-laboratory precision. The developed method can be used for the determination of N-dezacetyllappaconitine hydrobromide in a substance during quality control.
DEVELOPMENT OF METHODS OF STANDARDIZATION OF FLOWERS OF SPREADING MARIGOLD (TAGETES PATULA L.)
Authors: Kurkin Vladimir Aleksandrovich , Saveleva Anna Evgen'evna , Kurkina Anna Vladimirovna 
Published: December 2022

The spreading marigold (Tagetes patula L.), the family Aster (Asteraceae), or Composite (Compositae), is a prospective source of flavonoids. The aim of the study is to develop methods of standardization of marigold flowers rejected, consisting in determining the identity of this raw material and the quantitative determination of biologically active compounds. As a result of a comparative chromatographic study, the presence of flavonoids was detected upon detection at a wavelength of 254 and 366 nm and before and after reaction with an alcohol solution of aluminum chloride. The presence of patuletin and patulitrin was confirmed in extracts of different varietal forms of Tagetes patula L.. Using column chromatography on silica gel L 40/100, two flavonoid substances were isolated patuletin (3,5,7,3′,4′-pentahydroxy-6-methoxyflavone) and patulitrin (7-O-β-D-glucopyranoside of 3,5,7,3',4′-pentahydroxy-6-methoxyflavone), which have been identified by UV-, 1H-NMR -, 13C-NMR-spectroscopy and mass spectrometry, as well as acid hydrolysis. In the solvent system chloroform- ethanol-water (26 : 16 : 3) Rf values of patuletin and patulitrin are about 0.7 and 0.4, respectively. It was found that patulitrin is the main flavonoid in the flowers of spreading marigold and determines the character of the absorption curve of the electronic spectra of water-alcohol extraction from this raw material. During the quantitative analysis of the total flavonoids in the flowers of spreading marigold, it is necessary to recalculate the content of active substances to the dominant and diagnostically significant flavonoid patulitrin. It was determined that the content of the total flavonoids in the studied samples of flowers of different varieties of the spreading marigold varies from 4.36±0.02% to 11.71±0.05% (calculated on patulitrin).
Jornalismo e a falta de tempo: como a pressa na produção pode impactar o histórico papel de mediação
Authors: Carneiro Cristine Gerk Pinto 
Published: December 2022

O artigo discute os impactos da crise de tempo na produção jornalística, cada vez mais alinhada ao atual fluxo frenético e superficial da internet. Nesse sentido, a falta de investimento da força de trabalho em conteúdos com maior profundidade e contextualização enfraqueceria o histórico papel de mediação jornalística, e sua capacidade de divulgação de informações vinculadas a uma noção de longa duração. A partir da colaboração teórica de distintos autores que se debruçam sobre o tema, o texto discute como a desaceleração e o distanciamento de um chamado jornalismo declaratório poderiam ser um caminho de reencontro com a história da profissão e em direção a um futuro possível.
Some Insights Concerning the Halal Tourism Research. A Bibliometric Analysis
Authors: MAS’UD Riduan , SYAMSURRIJAL M. 
Published: December 2022

Halal tourism has become one of the topic issues that have a level of popularity among researchers in the scientific articles published in journals indexed in the Scopus database. This research study uses a bibliometric design analysis to map some insights related to the halal tourism by evaluating articles that have been published in journals indexed in Scopus database. Furthermore, data mining (knowledge discovery) techniques are used to analyze the titles and abstracts of 127 Scopus indexed journals that have been saved in the form of a CSV file. Finally, three analyzes are produced in this study: network visualization analysis, overlay visualization, and density visualization.
This study's results indicate a significantly increased of articles on halal tourism, especially in the Islamic marketing journals. Also, the main outputs obtained though this bibliometric research related to the halal tourism, reveal which is the most popular authors, which is the most cited articles, which is the country with most publications, which is the research domain most approached. In the same manner is underline which is less approached practices, i.e. , halal tourism from the perspective of Islamic law, etc.
Hotel Recovery Post Pandemic: Is Rebranding Required?
Authors: ERNAWATI Ni Made , VODĂ Mihai , JENDRA I Wayan , TANTRI Gusti Ayu Diah , AVRAM Eleonora Laura 
Published: December 2022

For hotel business to survive during COVID-19 Pandemic requires to conduct major adjustment in almost all aspects of operation. This qualitative study was conducted to assess the operation adjustment that may affect the hotel brand. Data was gathered from respondents comprising managers and decision-makers of 5-to-3-star hotels in Bali, Indonesia that remain open during the pandemic, using online survey and interview.
The results show that during the pandemic, hotels have made major operation adjustment to survive; whilst, maintaining the same quality standard, applying meticulous CHSE (Cleanliness Health Safety Environment) protocol, and keeping the customers informed through online promotion platforms regarding the hotel’s condition being ready to host guests. The adjustment made includes reducing operation cost, shifting target market into domestic market to maintain cash flow to cover operation, adjusting the rate and prices to adapt the weaker customer’s buying power, applying flexible booking and refund conditions, human resource policy and planning rearrangement, reducing the quantity of room offered and menu list items in Food and Beverage outlets. The hotel business resilient was owned to the professionalism of hoteliers, thus hotel rebranding is not required as the quality of the products offered is the same with those before the pandemic; at post-pandemic, it would be simply sifting the target market back to pre-pandemic.
Analysis of Planning and Drug Procurement at Kragan II Public Health Center during Covid-19 Pandemic
Authors: Maulidiyyah Fitrotul , Rahardjo Bambang Budi 
Published: December 2022

Introduction: Based on the results of a preliminary study at the Kragan II Health Center, Kragan District, Rembang Regency, it was found that problems in drug procurement activities were located on the Large Pharmaceutical Trader or Pedagang Besar Farmasi (PBF). In addition, the COVID-19 pandemic has also affected the budget cuts for the procurement of drugs at the Kragan II Health Center.Methods: This research was descriptive qualitative research. Data collection techniques use in-depth interviews and observation techniques. The sampling technique used was purposive sampling and data were analyzed using the Miles and Huberman model.Results: The results showed that the process of planning and supplying drugs at the Kragan II Health Center had not gone well even though the indicators in Regulation of the Minister of Health Number 74 of 2016 of the Republic of Indonesia had been realized. The process of planning and procuring drugs at the Kragan II Health Center (Puskesmas) experienced problems in the field of funding for the procurement of drugs due to the Covid-19 pandemic. Another problem is the PBF whose response is slow and the drugs distributed are not following what was ordered by the pharmacist.Conclusion: Regarding debts with PBF, pharmacists are advised to discuss with the Head of the Kragan II Health Center regarding budget priorities during the COVID-19 pandemic to immediately pay off debts with PBF so that pharmacists can procure with PBF again. Therefore, drug services to patients can run well.