bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of significant genes associated with prognosis of gastric cancer by bioinformatics analysis
Authors: Wang Shuanhu , Tao Song , Liu Yakui , Shi Yi , Liu Mulin 
Published: December 2022

Abstract
Background
Gastric cancer (GC) ranks second in mortality among all malignant diseases worldwide. However, the cause and molecular mechanism underlying gastric cancer are not clear. Here, we used integrated bioinformatics to identify possible key genes and reveal the pathogenesis and prognosis of gastric cancer.

Methods
The gene expression profiles of GSE118916, GSE79973, and GSE29272 were available from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) between GC and normal gastric tissues were screened by R software and Venn diagram software. GO and KEGG pathway enrichment of DEGs was performed using the DAVID database. A protein-protein interaction (PPI) network was established by STRING and visualized using Cytoscape software. Then the influence of hub genes on expression and survival was assessed using TCGA database.

Results
A total of 83 DEGs were found in the three datasets, including 41 up-regulated genes and 42 down-regulated genes. These DEGs were mainly enriched in extracellular matrix organization and cell adhesion. The enriched pathways obtained in the KEGG pathway analysis were extracellular matrix (ECM)-receptor interaction and focal adhesion. A PPI network of DEGs was analyzed using the Molecular Complex Detection (MCODE) app of Cytoscape. Four genes were considered hub genes, including COL5A1, FBN1, SPARC, and LUM. Among them, LUM was found to have a significantly worse prognosis based on TCGA database.

Conclusions
We screened DEGs associated with GC by integrated bioinformatics analysis and found one potential biomarker that may be involved in the progress of GC. This hub gene may serve as a guide for further molecular biological experiments.
Analgesic Effects of Regional Analgesic Techniques in Pediatric Inguinal Surgeries: A Systematic Review and Network Meta-Analysis of Randomized Controlled Trials
Authors: Hung Tsung-Yu , Bai Geng-Hao , Tsai Meng-Chen , Lin Ying-Chun 
Published: December 2022

BACKGROUND:
Various regional analgesic techniques have been used in pediatric inguinal surgery to facilitate postoperative recovery. However, each technique’s relative performance was undetermined owing to the lack of quantitative analysis.

METHODS:
We systematically searched MEDLINE, Cochrane Library, EMBASE, and Web of Science for randomized controlled trials that compared regional analgesia in pediatric inguinal surgeries. After critical study screening and selection, a random-effects network meta-analysis was performed. The primary outcome was the time to the first rescue analgesic after surgery, and the secondary outcomes were the number of patients requiring rescue analgesics after surgery, postoperative pain scores, incidence of postoperative nausea and vomiting, and other adverse events.

RESULTS:
This network meta-analysis included 69 randomized controlled trials (4636 patients) that compared 10 regional analgesic techniques. Our study revealed that the quadratus lumborum and transversus abdominis plane blocks had the longest time to the first rescue analgesic after pediatric inguinal surgeries, by 7.7 hours (95% confidence interval [CI], 5.0–10.3) and 6.0 hours (95% CI, 3.9–8.2) when compared with the control group, respectively. In the subgroup involving only inguinal hernia repair, the quadratus lumborum block significantly prolonged the time to the first rescue analgesic than all other regional analgesics. In contrast, in the subgroup involving orchidopexies, only the caudal block significantly prolonged the time to the first rescue analgesic when compared with the control group (4.1 hours; 95% CI, 0.7–7.5). Wound infiltration and landmark-based ilioinguinal-iliohypogastric block had relatively poor analgesic effects than other regional analgesics. No serious adverse effects related to the regional analgesic techniques were reported in any of the included studies.

CONCLUSIONS:
The quadratus lumborum and transversus abdominis plane blocks had the longest time to the first rescue analgesic and the least rescue analgesic requirement for pediatric inguinal surgeries. Specifically, the quadratus lumborum block had the longest analgesic duration in inguinal hernia repair, and the caudal block was found to be the only regional analgesia that extended the time to the first rescue analgesic in pediatric orchidopexy. Most included randomized controlled trials had some concern or a high risk of bias, and future studies should focus on providing high-quality evidence to further clarify the analgesic effects of regional analgesia for pediatric inguinal surgeries.
Two-Step Modification of Proteins onto Microparticle Surface Using DMT-MM Condensation Agent
Authors: Miyagawa Akihisa , Ueda Yasuyuki , Hagiya Kenta , Nagatomo Shigenori , Nakatani Kiyoharu 
Published: December 2022

Abstract
A two-step modification scheme using DMT-MM for protein modification on a microparticle surface is proposed. The reactions of myoglobin, lysozyme, and γ-immunoglobulin with carboxyl-functionalized polystyrene microparticles performed using DMT-MM were compared with those using EDC/NHS. The modification efficiency of proteins on the particles using DMT-MM was higher than that using EDC/NHS.
Aplikasi metode FTIR kombinasi kemometrika untuk analisis lemak daging tikus pada nugget ayam
Authors: Guntarti Any , Ahda Mustofa , Kusbandari Aprilia , Atmadja Dimas 
Published: December 2022

Indonesia termasuk di urutan ke 13 Â jumlah Â penduduk Â muslim terbesar di dunia, yang sangat Â memperhatikan halal haram pada makanan terutama nugget. Nugget merupakan makanan yang menjadi favorit bagi anak-anak. Beberapa pedagang dicurigai memalsukan atau Â mencampurkan nugget ayam dengan menggunakan daging lain, seperti daging tikus. Tujuan penelitian ini Â mengaplikasikan metode spektrofotometri Fourier Transform Infra Red dengan kemometrika untuk analisis lemak daging tikus pada nugget. Sampel referensi nugget ayam dan tikus dibuat variasi 7 konsentrasi, yaitu 0%, 25%, 35%, 50%, 65%, 75%, 100% dan 5 sampel kornet pasaran yang dibeli di wilayah Kecamatan Umbulharjo, Kota Yogyakarta. Selanjutnya dilakukan ekstraksi dengan Â Soxhlet, pelarut n-heksan teknis, 4-5 jam pada suhu 69-70oC untuk memperoleh lemak. Lemak dianalisis menggunakan metode spektrofotometri FTIR untuk menghasilkan data spektra inframerah. Data tersebut kemudian dianalis dengan kemometrika PLS dan PCA. Hasil Analisis diperoleh bilangan gelombang maksimal pada 900-1700 cm-1.Analisis kemometrika PLS (partial least square), diperoleh model analisis dengan nilai koefisien determinasi (R2) sebesar 0,9985 dengan persamaan y= 0,9898x + 0,3985 dan nilai parameter RMSEC 1,14%, RMSEP 0,67%, dan RMSECV 0,98%. Analisis dengan PCA (principal component analysis) antara nugget ayam 100% dan tikus 100% dapat dibedakan secara jelas. Â Metode spektrofometri FTIR dapat digunakan untuk aplikasi analisis lemak daging tikus pada nugget ayam, yang dikombinasikan kemometrika PLS dan PCA pada bilangan gelombang 900-1700 cm-1. Analisis PCA dengan 5 sampel pasaran menunjukkan bahwa nugget pasaran memiliki kemiripan sifat fisika kimia dengan nugget ayam 100%.Â
Is Virtual Reality a Game Changer in Pediatrics' Acute Pain Perception?
Authors: Mantegazza C. , Silvera V. , Brevini T. , Pozzi E. , Zuccotti GV. 
Published: December 2022

Purpose of review: Virtual reality (VR) is a promising tool that is becoming increasingly popular for the prevention and treatment of procedural pain in children. This review aims to investigate the current literature on the use and effectiveness of VR in paediatric procedural pain management, focusing on needle-related procedures, burn wound care, dental procedures among others; limitations and future perspectives on the use of VR will also be highlighted.
Recent findings: in addition to the most researched fields of application (e.g. needle procedures), VR has also started to be used in new areas, such as nasal and gastrointestinal endoscopies as well as minor surgeries. From the latest evidence, VR seems to be effective in many procedures, especially compared to no distraction; however, there is conflicting data in the literature due to numerous factors such as differences between technologies, degree of VR-immersion, patients coping styles among others.
Summary: there are still no data of absolute certainty on the effectiveness of VR in reducing procedural pain in paediatric patients and data pooling is still difficult. Future research should carry on large randomised-controlled, multicentre studies to better define VR properties and how best to optimise VR software and user experience for maximum pain reduction.
Design and assessment of a micro-nano positioning hexapod platform with flexure hinges for large aperture telescopes
Authors: Wang Xuewen , Yu Yang , Xu Zhenbang , Han Chunyang , Sun Jialin , Wang Jianli 
Published: December 2022

In order to compensate the optical system bias, which is caused by the change of elevation angle and thermal gradient during the optical alignment of the telescope, a novel high stiffness micro-nano positioning hexapod platform with flexure hinges is proposed in this paper. The novel flexure hinge has a mechanical limit, and its equivalent model is established and analyzed. In addition, in order to speed up the solution process, a novel simplified inverse kinematic model is developed based on the rigid body kinematic theory. Then, an effective rigid-flexible coupling simulation system is built to verify the correctness and applicability of the inverse kinematic model. Finally, a systematic experimental test method and a statistical-based data analysis theory are proposed. The experimental results show that the resolution and repeatability of translation and rotation and lateral stiffness are as follows: 0.3 mm and 0.5 arc sec, ± 0.5 µm and ±0.5 arc sec, 131.6N

⋅

µm-1 and 133.0N

⋅

µm-1. The proposed hexapod platform can be used to correct the optical system bias of large-aperture telescopes.
Serum Leucine-Rich α2 Glycoprotein: A Novel Biomarker for Transmural Inflammation in Crohn's Disease
Authors: Takenaka Kento , Kitazume Yoshio , Kawamoto Ami , Fujii Toshimitsu , Udagawa Yumi , Wanatabe Ryosuke , Shimizu Hiromichi , Hibiya Shuji , Nagahori Masakazu , Ohtsuka Kazuo , Sato Hiroyuki , Hirakawa Akihiro , Watanabe Mamoru , Okamoto Ryuichi 
Published: December 2022

INTRODUCTION:
Leucine-rich alpha-2 glycoprotein (LRG) is a newly studied biomarker for inflammatory diseases. This study aimed to investigate whether LRG can be used for evaluating transmural activity in patients with Crohn's disease (CD).

METHODS:
We performed magnetic resonance enterography (MRE) in 227 consecutive patients with CD from June 2020 to August 2021. We prospectively compared MRE findings with clinical and laboratory data including LRG. MRE was evaluated using 2 validated scoring systems, and transmural inflammation was defined as having a maximum simplified magnetic resonance index of activity (sMaRIA) score of ≥4 and a 5-point classification score of ≥9, respectively.

RESULTS:
The correlation between LRG and the total MRE score showed a positive correlation (r = 0.576 for the sMaRIA score, P < 0.01, and r = 0.633 for the 5-point score, P < 0.01). Serum concentrations of LRG significantly increased as MRE scores increased (P < 0.01). The area under the curve of LRG for a sMaRIA score of ≥4 and a 5-point score of ≥9 was 0.845 and 0.869, respectively, which was significantly higher than that of CDAI (P < 0.01) or C-reactive protein (P < 0.01). LRG levels of ≥14 μg/mL had a 67% sensitivity and 90% specificity for a sMaRIA score of ≥4 and a 73% sensitivity and 89% specificity for a 5-point score of ≥9. Patients with high LRG levels were also strongly associated with CD-related hospitalization, surgery, and clinical relapse compared with those with low LRG levels (P < 0.01 for all).

DISCUSSION:
LRG is a highly accurate serum biomarker for detecting transmural activity in patients with CD. Results need to be validated in further multicenter studies.
Subacute thyroiditis and its clinical characteristics — a retrospective single center clinical study
Authors: Manevska N. , Bozinovska N. , Stoilovska Rizova B. , Stojanoski S. , Makazlieva T. 
Published: December 2022

INTRODUCTION. Typical clinical features of subacute thyroiditis (SAT) are commonly assessed such as – neck pain and high body temperature, as well as thyroid function abnormalities, elevated inflammatory markers, and strongly suggestive hypoechoic ultrasonography characteristics. Non-steroid anti-inflammatory agents and corticosteroids are used.AIM. To examine the clinical characteristics in patients with SAT, by determining the level of thyroid hormones, the size and structure of the thyroid gland as well as ultrasound and scintigraphy findings.MATERIALS AND METHODS. We performed retrospective analysis in 122 cases of SAT (both genders, mean age 45.05±12,18 years), in the period 2015-2020. We evaluated monthly frequency of the SAT occurrence, the clinical status of the patients including symptoms, body temperature, laboratory results of FT4 and TSH, CRP and ESR level, ultrasonography and scintigraphy findings.RESULTS. Most of the patients complained of neck pain, high body temperature was detected in 66/100 (66%) pts. CRP and ESR Patients were usually diagnosed in hyperthyroid phase of the disease. Enlarged thyroid gland was mostly seen on US, while 52 had normal thyroid gland, with predominantly hypoechoic non-chomogenous structure. Scintigraphy noted “empty” scan (without presentation of functional thyroid tissue) in 72/98 (73,47%) and hypofixation mainly in both lobes in 26/98 (26,53%) pts.CONCLUSION. The awareness of physicians needs to be increased in patients with neck pain for proper diagnosis of SAT, that is often is often misdiagnosed or delayed, leading to erroneous antibiotic overuse. Generally, nonsteroidal anti-inflammatory drugs are effective in reducing thyroid pain in patients with mild cases.
Factors, associated with the outcomes of the thyrotoxic atrial fibrillation
Authors: Ponomartseva D. A. , Babenko A. Yu. 
Published: December 2022

BACKGROUND: Thyrotoxic atrial fibrillation (TAF) is associated with an increase in total and cardiovascular mortality, mainly due to the thromboembolic events. Therefore, thromboembolism prevention is an important TAF treatment component. Thus, it seems interesting to identify TAF thromboembolic complications predictors.TAF is a potentially reversible state. However, the spontaneous reversion to sinus rhythm after the euthyroidism achievement does not occur in all patients. In patients with a history of thyrotoxicosis the hospitalization rate due to cardiovascular pathology, and due to atrial fibrillation (AF), in particular, is higher than in the general population, even after the radical treatment of thyrotoxicosis.The development of prediction tools for mentioned above TAF complications and adverse outcomes, would make it possible to create more detailed and high-quality guidelines for the management of patients with thyrotoxicosis-induced AF. At the same time, the predictors of TAF thromboembolic complications and TAF maintenance after the euthyroid state is achieved, are not well currently understood.AIM: The purpose of this study was to identify risk factors for TAF adverse outcomes: thromboembolic events and the lack of spontaneous reversion to sinus rhythm after the euthyroidism was achieved.MATERIALS AND METHODS: This is a retrospective study of 70 patients aged 24 to 70 years with a history of overt thyrotoxicosis and TAF. The following parameters were analyzed in all participants: thromboembolic events due to TAF, the presence of the spontaneous reversion to sinus rhythm after the euthyroidism was achieved and potentially associated with TAF adverse outcomes factors: a number of clinical and demographic factors, echocardiography data and characteristics of the TAF course. Regression analysis was performed to study the effect of these potential predictors on the risk of the thromboembolism and TAF maintenance. The cut-off points for the identified risk factors were determined by receiver operating characteristic (ROC) curves creating.RESULTS: The analysis showed that the only independent TAF thromboembolic complications predictor among studied parameters was the large left atrium diameter (>4.3 cm), and the predictors of TAF maintenance after the euthyroid state is achieved included the large left ventricle end-diastolic size (>4.5 cm) and the presence of left atrium dilation.CONCLUSION: In this study, echocardiographic parameters associated with TAF thromboembolic complications and TAF persistence after euthyroid state is achieved, were identified. This may be useful for the TAF adverse outcomes risk assessment tools development in the future.
The System of Educational Needs of National Minorities in Education in Ukraine
Authors: TULIN Kyrylo 
Published: December 2022

The authors analyze the current system of educational needs of national minorities in Ukraine by defining the system of education, including legal policy, institutional and organizational guarantees, identifying problematic aspects in the sphere of Ukrainian education and identifying opportunities to overcome them, analyzing the status of educational needs of national minorities.
It is indicated that global transformations have intensified the tendencies of the number of national minorities due to global demographic processes, including migration and refugees.
It is argued that the elements of the system of educational needs of national minorities in education in Ukraine are international, European regional, and national legal acts, the functioning of public authorities, the complex structure of educational institutions at all levels, and additional organizational and economic guarantees of educational rights.
International, regional, and national legal norms are analyzed. Positive aspects and shortcomings of the legal regulation of the educational sphere of national minorities are indicated. A number of improvements to the current ethnopolitics of Ukraine in the context of the implementation of international and European standards and the positive practice of democratic states, including Romania, are proposed.
The methodological basis of the study is a system of philosophical, general scientific, and specifically scientific methods. The sociological survey, conducted on the author's questionnaire, represents the real state of national minorities education in Ukraine. The Russian, Hungarian, Slovak, and Polish national minorities are taken into account. The authors proved that the pandemic crisis has a particularly negative effect on the education of national minorities.
Efektifitas Penyisihan Fosfat Pada Air Limbah Menggunakan Metode Kombinasi Elektrokoagulasi dan Fotokatalis Dengan Kaolin-TiO2
Authors: Setiawan Adhi , Mahfudz Lintang Indra Liuqil , Ramadani Tarikh Azis , Muthi’ah Siti , Nizam Muhammad Asrul , Dermawan Denny 
Published: December 2022

Kandungan fosfat pada badan air salah satunya bersumber dari air limbah domestik dan industri. Fosfat yang berlebihan dapat menyebabkan eutrofikasi. Alternatif pengolahan fosfat pada air limbah salah satunya menggunakan kombinasi metode elektrokoagulasi dan fotokatalis. Penelitian ini bertujuan untuk mengetahui pengaruh waktu kontak dan besar tegangan pada metode elektrokoagulasi, pengaruh dosis katalis dan waktu penyinaran pada metode fotokatalis, dan karakteristik katalis komposit kaolin-TiO2. Penyisihan fosfat dilakukan secara batch menggunakan elektrokoagulasi yang dilanjutkan dengan proses fotokatalis. Metode elektrokoagulasi menggunakan elektroda aluminium dengan variasi tegangan 10, 20, dan 30 V, serta waktu kontak 90 dan 120 menit. Metode fotokatalis menggunakan komposit kaolin-TiO2 dan sumber foton lampu UV-C, dengan variasi dosis kaolin-TiO2 yaitu 120, 180, dan 240 mg/L, serta waktu penyinaran 10, 20 dan 30 menit. Komposit kaolin-TiO2 dikarakterisasi menggunakan XRD, FTIR, dan SEM-EDX. Hasil penelitian menunjukkan efisiensi penyisihan fosfat pada elektrokoagulasi maksimal diperoleh pada kondisi tegangan 30 V dan waktu kontak 120 menit dengan nilai sebesar 87,31%. Nilai maksimum penyisihan parameter fosfat fotokatalis terjadi pada dosis katalis 240 mg/L dengan waktu reaksi 30 menit dengan nilai sebesar 63,33%. One of the phosphate contents in water bodies is sourced from household and industrial wastewater. Excessive phosphates can cause eutrophication. One alternative of phosphate treatment in wastewater is the combination of electrocoagulation and photocatalytic methods. This study aims to determine the influence of contact time and voltage magnitude on the electrocoagulation method, the effect of catalyst dosing and irradiation time on the photocatalyst method, and the characteristics of kaolin-TiO2 composite catalysts. The electrocoagulation method uses aluminum electrodes with voltage variations of 10, 20, and 30 V, as well as contact times variations of 90 and 120 minutes. The photocatalyst method uses kaolin-TiO2 composite and UV-C lamp photon sources, with dose of kaolin-TiO2 variations of 120, 180, and 240 mg/L, as well as irradiation times variations of 10, 20 and 30 minutes. The kaolin-TiO2 composite is characterized by XRD, FTIR, and SEM-EDX. The results showed that the maximum efficiency of phosphate removal in electrocoagulation was obtained at a voltage of 30 V and a contact time of 120 minutes with a value of 87.31%. The maximum value of photocatalyst phosphate removal occurred at a catalyst dose of 240 mg/L with a reaction time of 30 minutes with a value of 63.33%.
“And Yet It Moves”: National Entrepreneurial Culture and Entrepreneurship-Friendly Policies: Evidence From OECD Countries
Authors: Poček Jasna , Fassio Claudio , Kraus Sascha 
Published: December 2022

Abstract
This paper investigates the impact of public policies aimed to foster entrepreneurship on the national entrepreneurial culture of a country. While most of the existing studies focus on the direct impact of policies on entrepreneurship outcomes, we propose that the impact of policies on entrepreneurial culture may be even more important for the development of economies in the long term. Using data for 36 OECD countries in the period 2002–2014, we investigate econometrically the impact of policies on culture usually associated with entrepreneurship and find that such impact is stronger for policies that address a broader target of potential beneficiaries, rather than a narrow one. Moreover, we find that, among the values that are usually associated with entrepreneurial activity, entrepreneurship-friendly policies foster those that are related to creativity, innovation and risk taking, but not those that relate to individualism and the belief that success is achieved through one’s own personal efforts. Lastly, we find that the positive impact of policies on culture only applies to the countries with a higher initial level of entrepreneurial culture, and not to the countries with lower initial levels.
The crystal structure of methyl 2-((4-chloro-2-fluoro-6-((2,2,2-trifluoroethyl) thio)phenoxy)methyl)benzoate, C17H13ClF4O3S
Authors: Liu Dongdong , Zhang Lixin , Zhang Jing 
Published: December 2022

AbstractC17H13ClF4O3S, monoclinic,P21/n(no. 14),a = 17.0097(10) Å,b = 4.4625(3) Å,c = 22.9520(19) Å,β = 102.933(7)°,V = 11698.0(2) Å3,Z = 4,Rgt(F) = 0.0606,wRref(F2) = 0.1605, T = 120 K.