bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stimulus-specific prediction error neurons in mouse auditory cortex
Authors: Audette Nicholas J. , Schneider David M. 
Published: January 2023

AbstractComparing expectation with experience is an important neural computation performed throughout the brain and is a hallmark of predictive processing. Experiments that alter the sensory outcome of an animal’s behavior reveal enhanced neural responses to unexpected self-generated stimuli, indicating that populations of neurons in sensory cortex may reflect prediction errors – mismatches between expectation and experience. However, enhanced neural responses to self-generated stimuli could also arise through non-predictive mechanisms, such as the movement-based facilitation of a neuron’s inherent sound responses. If sensory prediction error neurons exist in sensory cortex, it is unknown whether they manifest as general error responses, or respond with specificity to errors in distinct stimulus dimensions. To answer these questions, we trained mice to expect the outcome of a simple sound-generating behavior and recorded auditory cortex activity as mice heard either the expected sound or sounds that deviated from expectation in one of multiple distinct dimensions. Our data reveal that the auditory cortex learns to suppress responses to self-generated sounds along multiple acoustic dimensions simultaneously. We identify a distinct population of auditory cortex neurons that are not responsive to passive sounds or to the expected sound but that explicitly encode prediction errors. These prediction error neurons are abundant only in animals with a learned motor-sensory expectation, and encode one or two specific violations rather than a generic error signal.
The Ecology of Viruses in Urban Rodents with a Focus on SARS-CoV-2
Authors: Fisher Adam M. , Airey George , Liu Yuchen , Gemmell Matthew , Thomas Jordan , Bentley Eleanor G. , Whitehead Mark A. , Paxton William A. , Pollakis Georgios , Paterson Steve , Viney Mark 
Published: January 2023

AbstractWild animals are naturally infected with a range of viruses, some of which may be zoonotic for humans. During the human COIVD pandemic there was also the possibility of rodents acquiring SARS-CoV-2 from people, so-called reverse zoonoses. To investigate this we have sampled rats (Rattus norvegicus) and mice (Apodemus sylvaticus) from urban environments in 2020 during the human COVID-19 pandemic. We metagenomically sequenced lung and gut tissue and faeces for viruses, PCR screened for SARS-CoV-2, and serologically surveyed for anti-SARS-CoV-2 Spike antibodies. We describe the range of viruses that we found in these two rodent species. We found no molecular evidence of SARS-CoV-2 infection, though in rats we found lung antibody responses and evidence of neutralisation ability, that are consistent with rats being exposed to SARS-CoV-2 and / or exposed to other viruses that result in cross-reactive antibodies.
CTCF is essential for proper mitotic spindle structure and anaphase segregation
Authors: Chiu Katherine , Berrada Yasmin , Eskndir Nebiyat , Song Dasol , Fong Claire , Naughton Sarah , Chen Tina , Moy Savanna , Gyurmey Sarah , James Liam , Ezeiruaku Chimere , Capistran Caroline , Lowey Daniel , Diwanji Vedang , Peterson Samantha , Parakh Harshini , Burgess Ayanna R. , Probert Cassandra , Zhu Annie , Anderson Bryn , Levi Nehora , Gerlitz Gabi , Packard Mary C. , Dorfman Katherine A. , Bahiru Michael Seifu , Stephens Andrew D. 
Published: January 2023

AbstractMitosis is an essential process in which the duplicated genome is segregated equally into two daughter cells. CTCF has been reported to be present in mitosis but its importance for mitotic fidelity remains to be determined. To evaluate the importance of CTCF in mitosis, we tracked mitotic behaviors in wild type and two different CTCF CRISPR-based genetic knockdowns. We find that knockdown of CTCF results in prolonged mitoses and failed anaphase segregation via time lapse imaging of SiR-DNA. CTCF knockdown did not alter cell cycling or the mitotic checkpoint, which was activated upon nocodazole treatment. Immunofluorescence imaging of the mitotic spindle in CTCF knockdowns revealed disorganization via tri/tetrapolar spindles and chromosomes behind the spindle pole. Imaging of interphase nuclei showed that nuclear size increased drastically, consistent with failure to divide the duplicated genome in anaphase. Population measurements of nuclear shape in CTCF knockdowns do not display decreased circularity or increased nuclear blebbing relative to wild type. However, failed mitoses do display abnormal nuclear morphologies relative to successful mitoses, suggesting population images do not capture individual behaviors. Thus, CTCF is important for both proper metaphase organization and anaphase segregation which impacts the size and shape of the interphase nucleus.
Growth substrate limitation enhances anaerobic arsenic methylation byParaclostridium bifermentansstrain EML
Authors: Qiao Jiangtao , Sallet Hugo , Meibom Karin Lederballe , Bernier-Latmani Rizlan 
Published: January 2023

ABSTRACTMicrobial arsenic methylation is established as a detoxification process under aerobic conditions (converting arsenite to monomethylated arsenate) but proposed to be a microbial warfare strategy under anoxic conditions due to the toxicity of its main product monomethylarsonous acid (MMAs(III)). Here, we leveraged a paddy soil-derived anaerobic arsenic methylator,Paraclostridium bifermentansstrain EML, to gain insights into this process. Strain EML was inoculated into a series of media involving systematic dilutions of Reinforced Clostridial Broth (RCB) with 25 μM arsenite to assess the impact of growth substrate concentration on arsenic methylation. Growth curves evidenced the sensitivity of strain EML to arsenite, and As speciation analysis revealed the production of MMAs(III). Concentrations of MMAs(III) and arsenic methylation gene (arsM) transcription were found to be positively correlated with the RCB dilution, suggesting that substrate limitation enhancesarsMgene expression and associated anaerobic arsenic methylation. We propose that growth substrate competition between microorganisms may also lead to an increase in anaerobic As methylation. This hypothesis was further evaluated in an anaerobic co-couture mode of strain EML with either wild-typeEscherichia coliK-12 MG1655 (WT) orE. coliexpressing the MMAs(III)-resistance gene (arsP), (ArsPE. coli). We found increased MMAs(III) production in the presence ofE. colithan its absence and growth inhibition of WTE. colito a greater extent than ArsPE. coli, presumably due to MMAs(III) produced by strain EML. Taken together, our findings point to an ecological role for anaerobic arsenic methylation, highlighting the role of microbe-microbe competition/interaction in this process.IMPORTANCEAnaerobic arsenic methylation is enhanced in rice paddy soils under flooding conditions than that under drying conditions, leading to increased methylated arsenic accumulation in rice grains. Unlike the known detoxification role for aerobic arsenic methylation, the ecological role of anaerobic arsenic methylation remains elusive and is proposed to be an antibiotic-producing process involving in microbial warfare. In this study, we interrogated a rice paddy soil-derived anaerobic arsenic-methylating bacterium (Paraclostridium bifermentansstrain EML) to investigate the effect of growth substrate limitation on arsenic methylation by strain EML in the context of the microbial warfare hypothesis. We provide direct evidence for the role of growth substrate competition in anaerobic arsenic methylation by strain EML. Furthermore, we evidence a feedback loop, by which a bacterium resistant to MMAs(III) enhances its production, presumably through enhancedarsMexpression resulting from substrate limitation. Our work uncovers complex interactions between an anaerobic arsenic methylator and potential competitors.
13C and15N Resonance Assignments of Alpha Synuclein Fibrils Amplified from Lewy Body Dementia Tissue
Authors: Barclay Alexander M. , Dhavale Dhruva D. , Borcik Collin G. , Milchberg Moses H. , Kotzbauer Paul T. , Rienstra Chad M. 
Published: January 2023

AbstractFibrils of the protein α-synuclein (Asyn) are implicated in the pathogenesis of Parkinson Disease, Lewy Body Dementia, and Multiple System Atrophy. Numerous forms of Asyn fibrils have been studied by solid-state NMR and resonance assignments have been reported. Here, we report a new set of13C,15N assignments that are unique to fibrils obtained by amplification from postmortem brain tissue of a patient diagnosed with Lewy Body Dementia.
Major trends and environmental correlates of spatiotemporal shifts in the distribution of genes compared to a biogeochemical model simulation in the Chesapeake Bay
Authors: Preheim Sarah , Morris Shaina , Zhang Yue , Holder Chris , Arora-Williams Keith , Gensbigler Paul , Hinton Amanda , Jin Rui , Pradal Marie-Aude , Gnanadesikan Anand 
Published: January 2023

AbstractMicroorganisms mediate critical biogeochemical transformations that affect the productivity and health of aquatic ecosystems. Metagenomic sequencing can be used to identify how the taxonomic and functional potential of microbial communities change in response to environmental variables by investigating changes in microbial genes. However, few studies directly compare gene changes to biogeochemical model predictions of corresponding processes, especially in dynamic estuarine ecosystems. We aim to understand the major drivers of spatiotemporal shifts in microbial genes and genomes within the water column of the Chesapeake and highlight the largest discrepancies of these observations with model predictions. We used a previously published shotgun metagenomic dataset from multiple months, sites, and depths within Chesapeake Bay in 2017 and a metatranscriptomic dataset from 2010-2011. We compared metagenomic observations with rates predicted with a comprehensive physical-biogeochemical model of the Bay. We found the largest changes in the relative abundance of genes involved in carbon, nitrogen, and sulfur metabolism associated with variables that change with depth and season. Several genes associated with the largest changes in gene abundance are significantly correlated to corresponding modeled processes. Yet, several discrepancies in key genes were identified, such as differences between genes mediating nitrification, higher than expected abundance and expression of denitrification genes in aerobic waters, and nitrogen fixation genes in environments with relatively high ammonia but low oxygen concentrations. This study identifies processes that align with model expectations and others that require additional investigation to determine the biogeochemical consequences of these discrepancies and their impact within an important estuarine ecosystem.
Apo- and holo- transferrin differentially interact with ferroportin and hephaestin to regulate iron release at the blood-brain barrier
Authors: Baringer Stephanie L. , Palsa Kondaiah , Simpson Ian A. , Connor James R. 
Published: January 2023

AbstractBackgroundApo- (iron free) and holo- (iron bound) transferrin (Tf) participate in precise regulation of brain iron uptake at endothelial cells of the blood-brain barrier. Apo-Tf indicates an iron deficient environment and stimulates iron release, while holo-Tf indicates an iron sufficient environment and suppresses additional iron release. Free iron is exported through ferroportin, with hephaestin as an aid to the process. Until now, the molecular mechanism of apo- and holo-Tf’s influence on iron release was largely unknown.MethodsHere we use a variety of cell culture techniques, including co-immunoprecipitation and proximity ligation assay, in iPSC-derived endothelial cells and HEK 293 cells to investigate the mechanism of apo- and holo-Tf’s influence over iron release. We placed our findings in physiological context by further deciphering how hepcidin played a role in this mechanism as well.ResultsWe demonstrate that holo-Tf induces the internalization of ferroportin through the established ferroportin degradation pathway. Furthermore, holo-Tf directly binds to ferroportin, whereas apo-Tf directly binds to hephaestin. Only pathological levels of hepcidin disrupt the interaction between holo-Tf and ferroportin, and no amount of hepcidin disrupts the interaction between apo-Tf and hephaestin. The disruption of the holo-Tf and ferroportin interaction by hepcidin is due to hepcidin’s ability to rapidly internalize ferroportin compared to holo-Tf.ConclusionsThese novel findings provide a molecular mechanism for apo- and holo-Tf regulation of iron release from endothelial cells. They further demonstrate how hepcidin impacts these protein-protein interactions, and offer a model for how holo-Tf and hepcidin corporate to suppress iron release. We have established a more thorough understanding of the mechanisms behind iron release regulation with great clinical impact for a variety of neurological conditions in which iron release is dysregulated.
Ending the suitability quantification dilemma: intelligent decision support system for modular integrated construction in a high-density metropolis
Authors: Wuni Ibrahim Yahaya , Mazher Khwaja Mateen 
Published: January 2023

Purpose
Modular integrated construction (MiC) is a modern construction method innovating and reinventing the traditional site-based construction method. As it integrates advanced manufacturing principles and requires offsite production of volumetric building components, several factors and conditions must converge to make the MiC method suitable and efficient for building projects in each context. This paper aims to present a knowledge-based decision support system (KB-DSS) for assessing a project’s suitability for the MiC method.

Design/methodology/approach
The KB-DSS uses 21 significant suitability decision-making factors identified through literature review, consultation of experts and questionnaire surveys. It has a knowledge base, a DSS and a user interface. The knowledge base comprises IF-THEN production rules to compute the MiC suitability score with the efficient use of the powerful reasoning and explanation capabilities of DSS.

Findings
The tool receives the inputs of a decision-maker, computes the MiC suitability score for a given project and generates recommendations based on the score. Three real-world projects in Hong Kong are used to demonstrate the applicability of the tool for solving the MiC suitability assessment problem.

Originality/value
This study established the complex and competing significant conditions and factors determining the suitability of the MiC method for construction projects. It developed a unique tool combining the capabilities of expert systems and decision support system to address the complex problem of assessing the suitability of the MiC method for construction projects in a high-density metropolis.
Making sense of the digital badging landscape in education and workplace settings: a scoping review of the empirical literature
Authors: Cumberland Denise M. , Deckard Tyra G. , Kahle-Piasecki Lisa , Kerrick Sharon A. , Ellinger Andrea D. 
Published: January 2023

Purpose
The concept of digital badges (DBs) as a form of microcredentialing has gained considerable traction in higher education and workplace settings in recent years. This scoping review aims to map the empirical research conducted on DBs in higher education and workplace settings.

Design/methodology/approach
The design of this study is a scoping literature review. This scoping review adopts the five-stage scoping framework proposed by Arksey and O’Malley (2005).

Findings
Based upon our review of the 45 studies that comprised this scoping review of the empirical literature on DBs, we advance a typology that segments the empirical research based on whether DBs are used as pedagogical tools (PTs) or microcredentials. The authors found some confusion regarding nomenclature, numerous theories offered to explain DBs and divergent findings that suggest room for further exploration of this relatively new phenomenon.

Originality/value
This scoping review of the literature helps make sense of the emerging research landscape on DBs. The findings suggest that using DBs as a PT or as a microcredential has implications for a wide range of stakeholders regarding promoting lifelong learning, upskilling and reskilling the workforce. With the financial constraints facing higher education in a postpandemic environment, understanding the impact of DBs is needed before making an investment in this arena.
The socioemotional wealth of leaders in family firm succession and corporate governance processes: a systematic literature review
Authors: Marques Isabel Cristina Panziera , Leitão João , Ferreira João , Cavalcanti André 
Published: January 2023

PurposeAdopting the research concept of socioemotional wealth (SEW) as applied to family firm leaders, this study aims to analyse the literature on succession and corporate governance processes in family firms in keeping with this still developing concept and thereby not only contributing to advancing knowledge on this field but also proposing a conceptual model of analysis and a future research agenda.Design/methodology/approachThe authors carried out a review and systematisation of the literature according to its different thematic groups through recourse to VOSviewer software and content analysis to establish a systematised and integrated structure of the reference literature based on a sample of 218 studies published and indexed on the Web of Science and SCOPUS databases between 2010 and 2021.FindingsThe results portray five leading clusters, specifically, (1) SEW and family firm performance; (2) SEW and the focus on leadership as a precursor to succession; (3) family firms, SEW and corporate social governance; (4) family firm innovation and performance; and (5) family ownership and management. The authors then put forward a conceptual model of analysis both to better integrate these topics and as a proposition for application in future research projects.Originality/valueThe study provides a new and solid systematisation of the literature and supports the argument that family firms concentrating on developing the leader's SEW increase the probability of structuring a successful succession process as well as the likelihood of achieving higher quality corporate governance.
HRM solutions: a framework for small business owners to manage industrial relations while facilitating labour unionisation mitigation
Authors: LeCounte John Fitzpatrick 
Published: January 2023

Purpose
Small business owners require objective solutions to deal with threats of labour unionisation. This study aims to centre on a novel exploration for improving the leadership acumen of small business owners to address labour unionisation. Specifically, small business owners need a theoretical framework that uses best practices from human resource management (HRM) and industrial relations to provide solutions.

Design/methodology/approach
The design, methodology and approach reflect post-modernist epistemological and ontological perspectives for conducting systematic literature reviews. A systematic literature review revealed the relationship between small business owners’ leadership, positive industrial relations and HRM policies and procedures. To identify relevant studies in the review, the utilisation of several databases (EBSCO Database, including PsycINFO and Psych studies; Web of Science) and a mix of ranked journals from entrepreneurship, human resources, leadership and organisational behaviour.

Findings
The findings and results in this paper reflect the purpose, methodology and literature analysis culminating in 162,132,000 peer-reviewed studies. A total of 142 peer-reviewed studies met criterion for review. For example, the purpose of this review focused on labour unionisation mitigation for small businesses and HRM solutions. In doing so, the methodology allowed for identification of a novel research topic (i.e. how small business owners mitigate labour unionisation) worthy of further investigation. The sparse findings on labour unionisation mitigation represent small business owners' reticence in creating HRM policies and procedures.

Originality/value
This study contributes research implications for theory and practice by offering small business owners a theoretical framework to address labour unionisation. The framework, centring on HRM solutions, is grounded in social exchange theory to address the novel topic of labour unionisation mitigation in small businesses. This study results suggest that HRM solutions for small business owners provide competitive advantages for employee job satisfaction through a holistic industrial relations approach. In future research, examining other issues discussed in this study can influence the understanding of the relationship between small business owners and industrial relations to increase employee job satisfaction.
A meta-analysis of sustainable supply chain practices and performance: the moderating roles of type of economy and innovation
Authors: Yadav Sanjeev , Choi Tsan-Ming , Kumar Anil , Luthra Sunil , Naz Farheen 
Published: January 2023

PurposeIn recent years, sustainable supply chain practices (SSCPs), including corporate social responsibility (CSR), have been recognised as important means of developing firms’ sustainability performance (SP). However, empirical findings on the SSCP–SP interaction are inconsistent and even contradictory. This research presents a quantitative meta-analysis that aims to uncover SSCP–SP interactions based on the correlations found in previously published empirical studies.Design/methodology/approachBased on the main and moderating variables and selection criteria, 64 sample studies were selected after a systematic literature review and meta-analysis.FindingsThe findings confirm a positive correlation (0.438) between SSCP and SP. The results also reveal various critical moderators identified through meta-regression.Practical implicationsThis study provides insights for operations managers and policymakers regarding the significance of control variables (e.g. ISO certification, type of economy, innovation approach, data collection method) on the relationship between SSCP and SP for business operations. This research uncovers the impacts of ISO regulations and proposed hypotheses through the lens of the natural resource-based view (NRBV) and institutional-based view (IBV).Originality/valueThis research is unique in that it provides a systematic view of the SSCP–SP interaction, validates the results through a theoretical lens (NRBV and IBV) and generalises the results by evaluating the moderation effects via checking prior literature.