bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary research on dendritic cells loaded with resistant breast cancer antigens in breast cancer-bearing nude mice ⁎
Authors: Zhuang Wei , Lun Limin 
Published: June 2023

Abstract

Objective
The aim of the study was to investigate the inhibitory effects of dendritic cells (DCs) loaded with resistant breast cancer antigens on breast cancer in nude mice.

Methods
A single-cell suspension was prepared from a primary breast cancer and chemotherapeutic drugs were screened using the ATP-PCA susceptibility testing system. Cancer cells were treated with 1/10 × IC50, 1/5 × IC50, 1/2 × IC50, 1 × IC50, and 2 × IC50 medium until their growth became steady in the 2 × IC50 medium. Peripheral blood mononuclear cells (PBMCs) were obtained from the peripheral blood of patients with leukapheresis. The obtained adherent cells were induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) to generate DCs, which carried resistant strain cell lysis compounds or non-treated cancer cell lysis compounds. The former mature DCs carried resistant breast tumor antigens. A breast tumor-bearing nude mouse model was established with these resistant strains and the mice were randomly divided in three groups. The mice in the treatment group were injected with DCs loaded with resistant breast cancer antigens. The control group consisted of mice injected with DCs loaded with primary tumor cell antigens and the blank group consisted of mice injected with the same volume of normal saline. Changes in the cancers were observed.

Results
After treatment with the effector cells, the cancer volume and weight were significantly different to those before treatment in every group of mice (P < 0.05). The tumor volume in the blank group was the largest (3.362 ± 0.068 cm3) and the tumor weight was 637.50 ± 59.398 mg. Compared to the blank group, the tumor volume in the experimental group was the smallest (1.273 ± 0.071 cm3) and the tumor weight was 206.81 ± 32.711 mg.

Conclusion
DCs loaded with resistant breast cancer antigens demonstrated a significant inhibition effect on the cancers of breast tumor-bearing nude mice.
Expression of Bmi-1 and EZH2 in tissues adjacent to human epithelial ovarian cancer cells of orthotopic implantation in nude mice ⁎
Authors: Zhu Genhai , Hong Lan , Wang Shengtan , Yang Zhaoxin , Chen Chunying , Wang Shixuan 
Published: June 2023

Abstract

Objective
This study investigated the feasibility of screening residual normal ovarian tissues based on the expression of Bmi-1 and EZH2 in tissues adjacent to orthotopic ovarian carcinomas in nude mice.

Methods
The human epithelial ovarian cancer cell line OVCAR3 was grown in subcutaneous tissues and the tumor tissues were orthotopically implanted. The expression levels of Bmi-1 and EZH2 were detected by immunohistochemical staining and RT-PCR in cancer tissues, proximal and remote tissues with respect to the cancer tissues, and normal ovarian tissues of nude mice. Results Thirty-five ovarian tissue samples with normal biopsy results were obtained from 40 cases of human epithelial ovarian cancer in the nude mice in which the tumor tissues were orthotopically implanted. Bmi-1 and EZH2 expression levels were lower in proximal paraneoplastic tissue samples than in cancer tissue samples (P < 0.05) and higher than in remote paraneoplastic tissue samples (P < 0.01). No significant difference was found in the expression levels of Bmi-1 and EZH2 using immunohistochemistry among residual normal ovarian tissues obtained from orthotopically implanted models that differed in severity. The expression of Bmi-1 and EZH2 was negative in 20 normal ovarian tissue samples.

Conclusion
The expression levels of Bmi-1 and EZH2 were reduced with increasing distance from the cancer tissues. Negative expression of these tumor-associated genes can be used as a standard for the screening of normal ovarian tissues adjacent to tumor tissues. Normal ovarian tissues can be obtained from the tissues adjacent to tumors.
Clinical role of 18F-FDG PET/CT-based simultaneous modulated accelerated radiotherapy treatment planning for locally advanced nasopharyngeal carcinoma ⁎
Authors: Wang Jianshe , Tang Tianyou , Xu Jing , Wang Andrew Z. , Li Liang , Zheng Junnian , Zhang Longzhen 
Published: June 2023

Abstract

Objective
The aim of this study was to compare the long-term local control, overall survival, and late toxicities of positron emission tomography/computed tomography (PET/CT)-guided dose escalation radiotherapy versus conventional radiotherapy in the concurrent chemoradiotherapy treatment of locally advanced nasopharyngeal carcinoma (NPC).

Methods
A total of 48 patients with stage III-IVa NPC were recruited and randomly administered PET/CTguided dose escalation chemoradiotherapy (group A) or conventional chemoradiotherapy (group B). The dose-escalation radiotherapy was performed using the simultaneous modulated accelerated radiotherapy technique at prescribed doses of 77 gray (Gy) in 32 fractions (f) to the gross target volume (GTV): planning target volume (PTV) 1 received 64 Gy/32 f, while PTV2 received 54.4 Gy/32 f. Patients in group B received uniform-dose intensity-modulated radiotherapy, PTV1 received 70 Gy/35 f and PTV2 received 58 Gy/29 f. Concurrent chemotherapy consisted of cisplatin [20 mg/m2 intravenous (IV) on days 1-4] and docetaxel (75 mg/m2 IV on days 1 and 8) administered during treatment weeks 1 and 4. All patients received 2-4 cycles of adjuvant chemotherapy of the same dose and drug regimen.

Results
The use of fluorine-18-fluorodeoxyglucose (18F-FDG) PET/CT significantly reduced the treatment volume delineation of the GTV in 83.3% (20/24) of patients. The 5-year local recurrence-free survival rates of the two groups were 100% and 79.2%, respectively (P = 0.019). The 5-year disease free survival (DFS) rates were 95.8% and 75.0%, respectively (P = 0.018). The 5-year local progression-free survival and DFS rates were significantly different. The 5-year overall survival (OS) rates were 95.8% and 79.2%, respectively. Differences in OS improvement were insignificant (P = 0.079). Late toxicities were similar in the two groups. The most common late toxicities of the two arms were grade 1-2 skin dystrophy, xerostomia, subcutaneous fibrosis, and hearing loss. There were no cases of grade 4 late toxicity.

Conclusion
The use of 18F-FDG PET/CT-guided dose escalation radiotherapy is well tolerated and can reduce local recurrence rates for patients with locally advanced NPC compared to conventional chemoradiotherapy.
Survey of changes in dietary preferences in cancer patients in China
Authors: Li Jing , Zhang Liqiong , Tang Qiujun , Yuan Xianglin 
Published: June 2023

Abstract

Objective
The aim of this study was to investigate the changes in dietary preferences in cancer patients in China and to determine the need for encouraging the adherence to a sensible diet among such patients.

Methods
A total of 468 cancer patients were interviewed using a self-designed questionnaire focusing on changes in the intake of specific foods. Data were analyzed using SPSS 16.0.

Results
Most patients completely avoided roosters and carp (73.1%), condiments (51.9%), and meat of aquatic species (40.4%). All other types of the specific foods were completely avoided by different subpopulations of the patients.

Conclusion
In addition to focusing on disease treatment, medical professionals need to help cancer patients overcome barriers associated with the customs of avoiding specific foods encompassed by the term “fawu” and provide them with dietary guidance in order to prevent negative nutritional effects.
Progress of transformational therapy in colorectal liver metastases
Authors: Xiang Fang , Yin Xianli 
Published: June 2023

AbstractColorectal cancer liver metastases (CLM) treatment is very important given the high incidence of colorectal cancer with liver metastases, which are primarily treated by surgical resection. Transformational therapy such as systemic chemotherapy, hepatic arterial infusion (HAI), portal vein embolization (PVE), ablation therapy, and targeted therapy, should be applied to CLM patients who are unable to undergo immediate surgery to improve patients’ survival and quality of life.
Diaphragmatic suture with tubular stomach to prevent early delayed gastric emptying after esophagectomy
Authors: Sun Chao , Shi Weiping , Shu Yusheng , Shi Hongcan , Lu Shichun , Wang Kang 
Published: June 2023

Abstract

Objective
The objective of this study was to evaluate the clinical efficacy of a diaphragmatic suture with tubular stomach to prevent delayed gastric emptying (DGE) after esophagectomy through the cervico-thoracoabdominal approach.

Methods
A total of 980 patients with esophageal cancer undergoing esophagectomy through the cervico-thoracoabdominal approach were retrospectively included in this study and divided into two groups. All patients underwent tubular stomach creation (group A; n = 530) or a diaphragmatic suture and tubular stomach creation (group B; n = 450). The incidence of early DGE was observed.

Results
The incidence of early DGE in group A was significantly higher than that in group B (P < 0.05).

Conclusion
This observation study suggests that the use of a diaphragmatic suture with tubular stomach through the cervico-thoracoabdominal approach can decrease the incidence of early DGE after esophagectomy.
A clinicoepidemiological study of esophageal cancer patients at the National Cancer Institute, Cairo University, Egypt
Authors: Ezzat Soumaya , El Hossieny Hisham , Alla Mohamed Abd , Nasr Azza , Anter Nagwan , Adel Ahmed 
Published: June 2023

Abstract

Objective
The purposes of this study were to (1) assess the clinicoepidemiological characteristics of esophageal cancer patients, (2) analyze the prognostic factors determining treatment failure and survival, and (3) evaluate the results of various treatment modalities for locoregional and disseminated disease and their effect on disease-free survival and overall survival (OS).

Methods
Clinicoepidemiological retrospective data from 81 esophageal cancer patients treated at the National Cancer Institute of Cairo between 2007 and 2011 were evaluated.

Results
The study showed that patients with esophageal cancer commonly present with locally advanced disease (87.7% had T-stage 3 and 12.3% had T-stage 4). There was a significant correlation between surgery and survival; patients who received radical surgery and postoperative radiation had a better median survival than patients who received radical radiotherapy (20 months vs. 16 months, respectively; P = 0.04). There was also a significant statistical correlation between radical concomitant chemoradiotherapy (NCRT) and palliative treatment. Patients who received radical NCRT had a better median survival than patients who received palliative radiotherapy (16 months vs. 10 months, respectively; P = 0.001). The median follow-up period for all patients was 7 months. The median OS of the whole group was 12 months. The OS after 1 and 2 years was 57.8% and 15%, respectively.

Conclusion
High-dose NCRT is an acceptable alternative for patients unfit for surgery or with inoperable disease. High-dose radiation is more effective than low-dose radiation in terms of local control, time to relapse, and OS. Further study using a larger series of patients and introducing new treatment protocols is necessary for a final evaluation.
Imaging features of alveolar soft part sarcoma ⁎
Authors: Jin Teng , Zhang Ping , Li Xiaoming 
Published: June 2023

Abstract

Objective
The aim of this study was to analyze the imaging features of alveolar soft part sarcoma (ASPS).

Methods
The imaging features of 11 cases with ASPS were retrospectively analyzed. Results ASPS mainly exhibited an isointense or slightly high signal intensity on T1-weighted imaging (T1WI), and a mixed high signal on T2-weighted imaging (T2WI). ASPS was partial, with rich tortuous flow voids, or “line-like” low signal septa. The essence of the mass was heterogeneous enhancement. The 1HMRS showed a slight choline peak at 3.2 ppm.

Conclusion
The well-circumscribed mass and blood voids, combined with “line-like” low signals play a significant role in diagnosis. The choline peak and the other signs may be auxiliary diagnoses.
Low-dose radiation enhances susceptibility to cisplatin in resistant ovarian cancer cells via downregulation of ERCC1 and Bcl-2
Authors: Liu Xiaoran , Liang Donghai , Jiang Tao , Dong Qing , Yu Hongsheng 
Published: June 2023

Abstract

Objective
Ovarian cancer is one of the leading causes of mortality in patients with malignant gynecological tumors. After surgical intervention for ovarian cancer, cisplatin (DDP)-based chemotherapy is the first-line treatment. However, a major challenge to treating ovarian cancer is the development of chemoresistance. Thus, the first aim of this study was to determine whether low-dose radiation could enhance the susceptibility of resistant ovarian cancer cells to DDP. The second aim was to provide new strategies for treating DDP-resistant ovarian cancer by examining its mechanism.

Methods
A cell counting kit-8 (CCK8) assay was performed to measure cell proliferation. Flow cytometry was utilized to quantify the apoptosis of DDP-resistant ovarian cancer cells (SKOV3/DDP) using Annexin V and propidium iodide staining. Real-time quantitative (qPCR) was used to analyze the messenger RNA (mRNA) expression levels of excision repair cross complementing-group 1 (ERCC1) and B-cell lymphoma 2 (Bcl-2) in SKOV3/DDP.

Results
The IC50 values of the control, conventional-dose, and low-dose groups were 9.367 ± 0.16, 9.289 ± 0.16, and 3.847 ± 0.15, respectively (P < 0.05 vs control group and conventional-dose group). Compared with the control and conventional-dose groups, low-dose radiation resulted in significantly more apoptosis, as detected by flow cytometry (P < 0.05). The relative mRNA expression of ERCC1 and Bcl-2 in the low-dose group was significantly lower than that in the control group and conventional-dose group (P < 0.05).

Conclusion
Low-dose radiation enhanced the sensitivity of resistant ovarian cancer cells to DDP, possibly by decreasing the DNA repair capacity of tumor cells and promoting apoptosis.
Low-dose radiation reverses cisplatin resistance in ovarian cancer cells by changing Survivin and Caspase-3 expression
Authors: Dong Qing , Jiang Tao , Liang Donghai , Liu Xiaoran , Yu Hongsheng 
Published: June 2023

Abstract

Objective
Cisplatin (DDP) is the main chemotherapy drug for ovarian cancer. However, ovarian cancer cells tend to develop cisplatin resistance in the clinical setting. Tumor cells are sensitive to low-dose radiation (LDR). LDR therapy can improve the effects of chemotherapy drugs on ovarian cancer, but the underlying mechanisms are not clear. In this study, we explored the impact of low-dose radiation on Survivin and Caspase-3 levels in SKOV3/DDP ovarian cancer cells that are resistant to cisplatin.

Methods
Cell viability was examined by cell counting kit-8 (CCK-8) assay, and quantitative PCR was used to detect Caspase-3 and Survivin transcript levels. Flow cytometry was used to detect and quantify apoptotic cells. Results Cell viability was lower when cells were treated with LDR and cisplatin than when cells were treated with conventional radiation and cisplatin, or cisplatin alone (P < 0.05). The IC50 of cisplatin in the LDR, no-radiation control, and conventional-dose groups was 3.837 ± 0.16, 9.467 ± 0.17, and 9.389 ± 0.17, respectively. The level of Caspase-3 mRNA was higher and the level of Survivin mRNA was lower in the LDR group compared to that in the other two groups (P < 0.05).

Conclusion
LDR reverses cisplatin resistance in SKOV3/DDP cells, and may do so by suppressing Survivin expression and increasing Caspase-3 expression.
Targeting of RhoE inhibits epithelial-mesenchymal transition during colorectal cancer cell migration
Authors: Chen Gantao , Dong Weiguo 
Published: June 2023

AbstractObjectiveDespite microRNA (miR-200b) being proved to promote the proliferation of colorectal cancer (CRC) cells, the relationship between miR-200b and epithelial-mesenchymal transition (EMT) of CRC cells remains poorly understood. The aim of the study was to investigate the relationship between miR-200b and EMT during CRC cell migration.MethodsThe effect of miR-200b on EMT-associated markers E-cadherin and vimentin was evaluated by western blot in CRC cells (SW620 and HT-29) by treatment with miR-200b mimics and inhibitors. A luciferase reporter assay was employed to detect downstream targets of miR-200b. Transwell migration assays were used to detect CRC cell migration.ResultsWestern blots revealed that treatment with miR-200b mimics led to up-regulation of E-cadherin and down-regulation of vimentin, metalloproteinase (MMP)-9, and MMP-2, whereas treatment with miR-200b inhibitor exhibited opposite effects on expression of E-cadherin and vimentin. Luciferase reporter assays demonstrated that RhoE (RND3) was targeted by miR-200b. Two predicted target sites of miR-200b were present in the 3’-UTR of RhoE. Predicted target site 1 was from nucleotides 1584 to 1591, and site 2 was from nucleotides 1729 to 1735. RhoE knockdown cell lines were also established to investigate the impact of RhoE and miR-200b on EMT and cell migration. RhoE knockdown enhanced the effect of miR-200b mimics, up-regulating E-cadherin and down-regulating vimentin. RhoE knockdown also inhibited cell migration. Furthermore, miR-200b mimic treatment further promoted the inhibitory effect of RhoE knockdown on cell migration.ConclusionmiR-200b inhibited EMT and CRC cell migration partly via inhibiting RhoE expression in CRC. RhoE and miR-200b might therefore be promising target genes in the management of CRC.
The accuracy of magnetic resonance imaging and ultrasound in evaluating the size of early-stage breast neoplasms
Authors: Wang Zheng , Chen Hongzhi , Ma Xiaobin , Dai Zhijun , Lin Shuai , Kang Huafeng 
Published: June 2023

Abstract

Objective
Breast cancer is the most frequently diagnosed cancer in women. Accurate evaluation of the size and extent of the tumor is crucial in selecting a suitable surgical method for patients with breast cancer. Both overestimation and underestimation have important adverse effects on patient care. This study aimed to evaluate the accuracy of breast magnetic resonance imaging (MRI) and ultrasound (US) examination for measuring the size and extent of early-stage breast neoplasms.

Methods
The longest diameter of breast tumors in patients with T1-2N0-1M0 invasive breast cancer preparing for breast-conserving surgery (BCS) was measured preoperatively by using both MRI and US and their accuracy was compared with that of postoperative pathologic examination. If the diameter difference was within 2 mm, it was considered to be consistent with pathologic examination.

Results
A total of 36 patients were imaged using both MRI and US. The mean longest diameter of the tumors on MRI, US, and postoperative pathologic examination was 20.86 mm ± 4.09 mm (range: 11-27 mm), 16.14 mm ± 4.91 mm (range: 6-26 mm), and 18.36 mm ± 3.88 mm (range: 9-24 mm). US examination underestimated the size of the tumor compared to that determined using pathologic examination (t = 3.49, P < 0.01), while MRI overestimated it (t = −6.35, P < 0.01). The linear correlation coefficients between the image measurements and pathologic tumor size were r = 0.826 (P < 0.01) for MRI and r = 0.645 (P < 0.01) for US. The rate of consistency of MRI and US compared to that with pathologic examination was 88.89% and 80.65%, respectively, and there was no statistically significant difference between them (χ2 = 0.80, P > 0.05).

Conclusion
MRI and US are both effective methods to assess the size of breast tumors, and they maintain good consistency with pathologic examination. MRI has a better correlation with pathology. However, we should be careful about the risk of inaccurate size estimation.
Efficacy and time course of palliative radiotherapy for pain relief in 70 patients with bone metastases ⁎
Authors: Zhang Peng , Gong Chen , Xiong Huihua 
Published: June 2023

Abstract

Objective
The aim of this study was to evaluate the efficacy and time course of radiotherapy for pain relief in patients with bone metastases.

Methods
A total of 70 patients with painful bone metastases were investigated between January 2013 and August 2015. The patients were divided into 3 groups and each group was treated with radiotherapy using 30 Gy in 10 fractions, 20 Gy in 5 fractions, or a single dose of 8 Gy. The pain over the irradiated site was assessed using a numerical rating scale (NRS) ranging from 0 to 10. Pain relief was assessed every 5 days based on a pain questionnaire.

Results
Complete pain relief was achieved in 14.3% (10/70) patients; partial pain relief in 74.3% (52/70); and no response in 11.4% (8/70). The overall response rate was 88.6%. No significant difference was observed between single fraction radiotherapy and multifraction radiotherapy. There was no relationship between the pain relief and treated sites. The pain score gradually decreased and most patients reached a moderate pain score (NRS < 7) on day 10-15.

Conclusion
Local radiotherapy is a very rapid and effective palliative treatment for painful bone metastases; however, the optimal dose and fractionation regimen remain debatable. Individualized therapy for painful bone metastases should be considered according to the patient’s condition and life expectancy.
Prophylaxis and management of perioperative hemorrhage in retropubic radical prostatectomy
Authors: Pan Dongliang 
Published: June 2023

Abstract
Retropubic radical prostatectomy (RRP) has commonly been performed for localized prostate cancer. In the past decade, with the development of laparoscopic and robotic techniques, laparoscopic radical prostatectomy (LRP) or robotic-assisted LRP has been widely used due to its less invasive nature along with its shorter recovery, reduced blood loss, and improved visualization of the operative region compared to open techniques. Severe hemorrhage following prostatectomy is relatively rare (0.5% to 1.6%), but it is a serious complication. Here, we summarize circumstances in which perioperative severe hemorrhage can develop and interventions needed to achieve hemostasis.