bluuebunny/crossref_metadata_2025_split huggingface dataset:
Socio-ecological factors associated with probable depression among pregnant and parenting adolescent girls: findings from a cross-sectional study in Burkina Faso and Malawi
Authors: Ajayi Anthony Idowu , Chamdimba Elita , Sawadogo Nathalie , Gitahi Nyawira , Tarnagda Abdoul Moumini , Ilboudo Abdoul Kader , Munthali Alister , Thakwalakwa Chrissie , Otukpa Emmanuel Oloche , Ushie Boniface Ayanbekongshie , Kabiru Caroline W. 
Published: April 2023

Abstract
Background
Pregnant and parenting adolescent girls are at risk of poor mental health because of stigma and social exclusion. Despite one in four girls starting childbearing by the age of 19 in Africa, no study, to the best of our knowledge, has examined the multi-layered factors (individual, family, friends, and neighborhood-related factors) associated with depressive symptoms among pregnant and parenting girls in Africa. Our study contributes to addressing this gap by examining the socio-ecological factors associated with depression symptoms among pregnant and parenting adolescent girls.

Methods
Our study adopted a cross-sectional design. Between March and September 2021, we interviewed 980 pregnant and parenting adolescent girls in Ouagadougou, Burkina Faso, and 669 in Blantyre, Malawi. We recruited pregnant and parenting adolescent girls in randomly selected urban and rural enumeration areas in Burkina Faso (n = 71) and Malawi (n = 66). We assessed depressive symptoms using the Patient Health Questionnaire (PHQ-9), which generated an overall score of 27. We considered a score of 10 or more as probable depression. We also obtained information on individual, family, friends, and neighborhood characteristics. We employed logistic regression models to examine the significant factors associated with probable depression among pregnant and parenting adolescent girls.

Results
The prevalence of probable depression was 18.8% and 14.5% in Burkina Faso and Malawi, respectively. At the individual level, having secondary education was significantly associated with a lower likelihood of probable depression in Malawi (AOR: 0.47; 95% CI 0.27–0.82) but not in Burkina Faso. At the family level, denying paternity (AOR: 3.14; 95% CI 1.34–7.11 in Malawi) and no parental support (AOR: 2.08; 95% CI 1.22–3.55 in Burkina Faso) were associated with higher odds of probable depression. At the community level, perceived neighborhood safety was associated with a lower likelihood of probable depression in Malawi (AOR: 0.74; 95% CI 0.61–0.89) and Burkina Faso (AOR: 0.81; 95% CI 0.73–0.90). Having a safety net within the community was associated with lower odds of probable depression in Burkina Faso (AOR: 0.87; 95% CI 0.78–0.96) but not in Malawi.

Conclusion
Depressive symptoms are common among pregnant and parenting adolescents, suggesting the need to screen them regularly for depression during antenatal and postnatal visits. Factors associated with depression among pregnant and parenting girls operate at multiple levels suggesting a need for multilevel interventions that address all areas of vulnerabilities.
Carriers of the p.P522R variant in PLCγ2 have a slightly more responsive immune system
Authors: Diks Annieck M. , Teodosio Cristina , de Mooij Bas , Groenland Rick J. , Naber Brigitta A. E. , de Laat Inge F. , Vloemans Sandra A. , Rohde Susan , de Jonge Marien I. , Lorenz Linda , Horsten Debbie , van Dongen Jacques J. M. , Berkowska Magdalena A. , Holstege Henne 
Published: April 2023

Abstract
Background
The rs72824905 single-nucleotide polymorphism in the PLCG2 gene, encoding the p.P522R residue change in Phospholipase C gamma 2 (PLCγ2), associates with protection against several dementia subtypes and with increased likelihood of longevity. Cell lines and animal models indicated that p.P522R is a functional hypermorph. We aimed to confirm this in human circulating peripheral immune cells.

Methods
We compared effects of p.P522R on immune system function between carriers and non-carriers (aged 59-103y), using in-depth immunophenotyping, functional B-cell and myeloid cell assays, and in vivo SARS-CoV-2 vaccination.

Results
In line with expectations, p.P522R impacts immune cell function only slightly, but it does so across a wide array of immune cell types. Upon B-cell stimulation, we observed increased PLCγ2 phosphorylation and calcium release, suggesting increased B-cell sensitivity upon antigen recognition. Further, p.P522R-carriers had higher numbers of CD20++CD21-CD24+ naive B cells and IgG1+ memory B cells. In myeloid cells, normalized ROS production was higher upon PLCγ2-dependent stimulation. On classical monocytes, CD33 levels were elevated. Furthermore, carriers expressed lower levels of allergy-related FcεRI on several immune cell subsets. Nevertheless, carriers and non-carriers had similar serological responses to SARS-CoV-2 vaccination.

Conclusion
The immune system from p.P522R-carriers is slightly more responsive to stimulation than in non-carriers.
Quantitative prediction of radiographic progression in patients with axial spondyloarthritis using neural network model in a real-world setting
Authors: Baek In-Woon , Jung Seung Min , Park Yune-Jung , Park Kyung-Su , Kim Ki-Jo 
Published: April 2023

AbstractBackgroundPredicting radiographic progression in axial spondyloarthritis (axSpA) remains limited because of the complex interaction between multiple associated factors and individual variability in real-world settings. Hence, we tested the feasibility of artificial neural network (ANN) models to predict radiographic progression in axSpA.MethodsIn total, 555 patients with axSpA were split into training and testing datasets at a 3:1 ratio. A generalized linear model (GLM) and ANN models were fitted based on the baseline clinical characteristics and treatment-dependent variables for the modified Stoke Ankylosing Spondylitis Spine Score (mSASSS) of the radiographs at follow-up time points. The mSASSS prediction was evaluated, and explainable machine learning methods were used to provide insights into the model outcome or prediction.ResultsTheR2values of the fitted models were in the range of 0.90–0.95 and ANN with an input of mSASSS as the number of each score performed better (root mean squared error (RMSE) = 2.83) than GLM or input of mSASSS as a total score (RMSE = 2.99–3.57). The ANN also effectively captured complex interactions among variables and their contributions to the transition of mSASSS over time in the fitted models. Structural changes constituting the mSASSS scoring systems were the most important contributing factors, and no detectable structural abnormalities at baseline were the most significant factors suppressing mSASSS change.ConclusionsClinical and radiographic data-driven ANN allows precise mSASSS prediction in real-world settings. Correct evaluation and prediction of spinal structural changes could be beneficial for monitoring patients with axSpA and developing a treatment plan.
Hemolysis and acquired methemoglobinemia associated with lidocaine and benzocaine topical application: a case report
Authors: Khajavirad Nasim , Daftari Ghazal , Jelodar Mehrasa Raisi 
Published: April 2023

Abstract
Background
Topical anesthetics are commonly used over the counter, and one of the adverse effects of these medications is methemoglobinemia, which is a serious and life-threatening condition.

Case presentation
We describe a 25-year-old Persian male presenting with generalized weakness, dizziness, headache, and cyanosis. In addition, he had genital warts starting 3 weeks ago, which were self-treated with podophyllin, resulting in itching and pain. He used over-the-counter topical anesthetics, including benzocaine and lidocaine, to reduce the symptoms. According to the lab data, signs and symptoms of methemoglobinemia and hemolysis were diagnosed. Considering the hemolysis, ascorbic acid was used for treatment. The patient was discharged after 5 days with normal arterial blood gas and pulse oximetry and no signs and symptoms.

Conclusion
This case highlights that self-administration of some topical anesthetics may lead to potentially fatal conditions.
Delayed reversal of methanol-induced blindness in an adult by a combination of erythropoietin and a high dose of methylprednisolone: a case report
Authors: Vafapour Fatemeh , Jahangiri-Mehr Sajad , Hassanzadeh Sajad 
Published: April 2023

AbstractBackgroundThe clinical manifestation following methanol toxicity accounts for a life-threatening problem that contributes to metabolic disorders, neurological complications, blindness, and even death. There is no completely effective treatment to retain the patient’s vision. Herein, we apply a new therapeutic strategy for the recovery of bilateral blindness in a patient who had ingested methanol.Case presentationA 27-year-old Iranian man with complete bilateral blindness was referred 3 days after accidental ingestion of methanol to the poisoning center at Jalil Hospital, Yasuj, Iran, in 2022. After taking his medical history, performing neurologic and ophthalmologic examinations, and routine laboratory tests, ordinary management was undertaken and counterpoisons were given for 4–5 days; however, the blindness did not reverse. Following the 4–5 days of unsuccessful standard management, he was given ten doses of subcutaneous erythropoietin 10,000 IU/12 hours twice daily, folinic acid 50 mg/12 hours, and methylprednisolone 250 mg/6 hours for 5 days. After five days, vision of both eyes recovered, reaching 1/10 in the left and 7/10 in the right eye. He remained under daily supervision until his release from the hospital, and he was discharged from the hospital 15 days post admission. In outpatient follow-up, his visual acuity was improved without having any side effects at 2 weeks after discharge.ConclusionA combination of erythropoietin and a high dose of methylprednisolone were useful for relieving the critical optic neuropathy and improved the optical neurological disorder following methanol toxicity.
Analysis of rural–urban vegetable market dynamics in Central Gondar Zone, Ethiopia
Authors: Adugna Marelign , Tilahun Assefa , Petros Tigist , Adino Kibrom 
Published: April 2023

Abstract
Background
Vegetable crops are among most perishable agricultural products which require efficient marketing system to reach them fresh to consumers. Weak market integration and sluggish price transmission of such perishable products can lead to suboptimal allocation of resources. This study was aimed to identify the degree of vegetable price integration and speed of price transmission between urban and rural vegetable markets.

Methods
Two rural markets (Maksegnit and Koladiba) and one central urban market (Gondar) were selected for the purpose of the analysis based on their strong relationship as supply and destination market for vegetable crops. Price data of vegetable crops were collected from September 2013 to December 2018 from each market areas trade and revenue department offices. Augmented Dickey-Fuller test, Granger causality test and error correction model were utilized in the process of the analysis.

Results
The result shows that both rural markets were integrated with urban market for garlic crop. In addition, the rural markets caused the urban market to change not in the reverse direction. Moreover, urban market is adjusted by about 30% per month when there were a 1% price shock in rural markets for garlic crop. This speed of price transmission is sluggish for perishable crops and seasonally flexible prices.

Conclusion
This study concluded that vegetable prices are determined from demand side and it should be the target for any planned policy modification.
Anaerobic degradation of organic carbon supports uncultured microbial populations in estuarine sediments
Authors: Yu Tiantian , Wu Weichao , Liang Wenyue , Wang Yinzhao , Hou Jialin , Chen Yunru , Elvert Marcus , Hinrichs Kai-Uwe , Wang Fengping 
Published: April 2023

Abstract
Background
A large proportion of prokaryotic microbes in marine sediments remains uncultured, hindering our understanding of their ecological functions and metabolic features. Recent environmental metagenomic studies suggested that many of these uncultured microbes contribute to the degradation of organic matter, accompanied by acetogenesis, but the supporting experimental evidence is limited.

Results
Estuarine sediments were incubated with different types of organic matters under anaerobic conditions, and the increase of uncultured bacterial populations was monitored. We found that (1) lignin stimulated the increase of uncultured bacteria within the class Dehalococcoidia. Their ability to metabolize lignin was further supported by the presence of genes associated with a nearly complete degradation pathway of phenolic monomers in the Dehalococcoidia metagenome-assembled genomes (MAGs). (2) The addition of cellulose stimulated the increase of bacteria in the phylum Ca. Fermentibacterota and family Fibrobacterales, a high copy number of genes encoding extracellular endoglucanase or/and 1,4-beta-cellobiosidase for cellulose decomposition and multiple sugar transporters were present in their MAGs. (3) Uncultured lineages in the order Bacteroidales and the family Leptospiraceae were enriched by the addition of casein and oleic acid, respectively, a high copy number of genes encoding extracellular peptidases, and the complete β-oxidation pathway were found in those MAGs of Bacteroidales and Leptospiraceae, respectively. (4) The growth of unclassified bacteria of the order Clostridiales was found after the addition of both casein and cellulose. Their MAGs contained multiple copies of genes for extracellular peptidases and endoglucanase. Additionally, 13C-labeled acetate was produced in the incubations when 13C-labeled dissolved inorganic carbon was provided.

Conclusions
Our results provide new insights into the roles of microorganisms during organic carbon degradation in anaerobic estuarine sediments and suggest that these macro and single molecular organic carbons support the persistence and increase of uncultivated bacteria. Acetogenesis is an additional important microbial process alongside organic carbon degradation.
Characterisation of a new lightweight LoRaWAN GPS bio-logger and deployment on griffon vultures Gyps fulvus
Authors: Gauld Jethro , Atkinson Philip W. , Silva João P. , Senn Andreas , Franco Aldina M. A. 
Published: April 2023

Abstract1. Information provided by tracking studies using remote telemetry is providing ecologists with invaluable new insights into animal behaviour and movement strategies. Here we describe a new type of GNSS (Global Navigation Satellite System) tracking device currently under development and nearing commercialisation, which transmits data via LoRaWAN (long range wide area network) gateways. These tags have the potential to be a low weight and power consumption solution for tracking the movement of animals at high resolution. 2. We characterise the position accuracy and data transmission range, including uplinks and downlinks, for the tracker using a series of ground-based field tests. Data transmission range was tested by visiting locations with line of sight to the LoRaWAN Gateway at distances up to 75 km and recording whether data transmission was completed successfully from each location. These tests were complemented by a trial deployment of six devices on griffon vulturesGyps fulvus. 3. These LoRa tags reliably provided accurate position estimates, particularly on more frequent acquisition cycles. At 1-min intervals the GNSS location bias was 4.71 m in the horizontal plane and 5 m in the vertical plane while precision, measured by standard deviation, was 3.9 m in horizontal space and 7.7 m in vertical space. Ground-based range tests confirmed data transmission from a maximum distance of 40.7 km. Initial results from a deployment on griffon vultures yielded useful information about flight speeds, altitude, and transmission range (up to 53.4 km). 4. With consistent GNSS position accuracy and the ability to transmit data over tens of kilometres, the LoRa tags demonstrated potential for monitoring animal movement over large areas. The small size and power needs of the device allow for flexibility in which combination of battery, solar panel, and housing they are paired with. The tags can be assembled in housing formats ranging in size from less than 5 g for deployment on Kestrel sized birds to 80 g for deployment on large birds such as vultures. The devices are particularly suitable for philopatric (site-faithful) species because LoRa gateways can be installed near breeding sites to maximise opportunities for data transmission. Our findings are informative for studies seeking to use LoRa for tracking birds and other animals using the miro-Nomad or a different type of GPS-LoRa logger.
Implementing evidence-based eating disorder guidelines at a small metro hospital: current practice and staff perceptions of caring for eating disorder patients
Authors: O’Donnell Stacey L. , Meloncelli Nina J. L. 
Published: April 2023

Abstract
Background
The aim of this study was to understand current clinical practice, adherence to evidence-based guidelines, and the perceptions, knowledge and attitudes of the multidisciplinary team caring for inpatients with an eating disorder at a small metro hospital.

Methods
This mixed methods study involved a retrospective audit of eating disorder patient care and a semi-qualitative staff survey. The audit was undertaken at a small metro hospital from 2018 to 2019. Documented practices were compared to state-wide best-practice guidelines. A staff survey was designed to understand health care professional’s knowledge and use of evidence-based practice guidelines, as well their perception of caring for this patient population and areas for improvement.

Results
Twenty-three discrete admissions (18 individuals) were included in the audit. Findings highlighted several evidence-practice gaps including delayed nutrition initiation and inconsistent medical refeeding and management of refeeding risk. Survey themes (from 60 hospital staff) included: lack of confidence with providing eating disorder care; uncertainty about professional roles/responsibilities; and lack of clear processes/guidelines to inform clinical care.

Conclusions
Gaps exist between evidence-based practice and eating disorder patient care. Staff lack confidence providing care to this patient group. These findings will allow for targeted implementation strategies to improve patient care and the uptake of research into practice.
Experiences that matter in bipolar disorder: a qualitative study using the capability, comfort and calm framework
Authors: Siegel-Ramsay J. E. , Sharp S. J. , Ulack C. J. , Chiang K. S. , Lanza di Scalea T. , O’Hara S. , Carberry K. , Strakowski S. M. , Suarez J. , Teisberg E. , Wallace S. , Almeida J. R. C. 
Published: April 2023

Abstract
Background
When assessing the value of an intervention in bipolar disorder, researchers and clinicians often focus on metrics that quantify improvements to core diagnostic symptoms (e.g., mania). Providers often overlook or misunderstand the impact of treatment on life quality and function. We wanted to better characterize the shared experiences and obstacles of bipolar disorder within the United States from the patient’s perspective.

Methods
We recruited 24 individuals diagnosed with bipolar disorder and six caretakers supporting someone with the condition. Participants were involved in treatment or support services for bipolar disorder in central Texas. As part of this qualitative study, participants discussed their everyday successes and obstacles related to living with bipolar disorder during personalized, open-ended interviews. Audio files were transcribed, and Nvivo software processed an initial thematic analysis. We then categorized themes into bipolar disorder-related obstacles that limit the patient’s capability (i.e., function), comfort (i.e., relief from suffering) and calm (i.e., life disruption) (Liu et al., FebClin Orthop 475:315–317, 2017; Teisberg et al., MayAcad Med 95:682–685, 2020). We then discuss themes and suggest practical strategies that might improve the value of care for patients and their families.

Results
Issues regarding capability included the struggle to maintain identity, disruptions to meaningful employment, relationship loss and the unpredictable nature of bipolar disorder. Comfort related themes included the personal perception of diagnosis, social stigma and medication issues. Calm themes included managing dismissive doctors, finding the right psychotherapist and navigating financial burdens.

Conclusions
Qualitative data from patients with bipolar disorder helps identify gaps in care or practical limitations to treatment. When we listen to these individuals, it is clear that treatments must also address the unmet psychosocial impacts of the condition to improve patient care, capability and calm.
Resource predictability modulates spatial-use networks in an endangered scavenger species
Authors: Cerecedo-Iglesias Catuxa , Bartumeus Frederic , Cortés-Avizanda Ainara , Pretus Joan Ll. , Hernández-Matías Antonio , Real Joan 
Published: April 2023

Abstract
Background
Changes in human-induced resource availability can alter the behaviour of free-living species and affect their foraging strategies. The future European Landfill Waste Directive and Circular Economy Action Plan will reduce the number of predictable anthropogenic food subsidies (PAFS), above all, by closing landfills to preclude negative effects on human health. Obligate avian scavengers, the most threatened group of birds worldwide, are the most likely group of species that will be forced to change their behaviour and use of space in response to landfill site closures. Here, we examine the possible consequences of these management decisions on the foraging patterns of Egyptian vultures (Neophron percnopterus) in an expanding population in the Iberian Peninsula.

Methods
We tracked 16 individuals in 2018–2021, including breeders and non-breeders, and, using a combination of spatial-use and spatial-network modelling, assessed landscape connectivity between key resources based on movement patterns. We then carried out simulations of future scenarios based on the loss of PAFS to predict likely changes in the movement patterns of both non-breeders and breeders.

Results
Our results show that foraging strategies in non-breeders and breeders differ significantly: non-breeders performed more dispersal movements than breeding birds across a spatial-use network. Non-breeding and breeding networks were found to be vulnerable to the removal of central foraging areas containing landfill sites, a highly predictable resource, while perturbation analysis showed dissimilar foraging responses to the gradual reduction of other predictable resources. Under a context of the non-availability of landfills for breeders and non-breeders, vultures will increase their use of extensive livestock as a trophic resource.

Conclusions
Future environmental policies should thus extend the areas used by scavengers in which livestock carcasses are allowed to remain in the wild, a strategy that will also mitigate the lack of food caused by any reduction in available waste if landfills close. In general, our results emphasize the capabilities of a spatial network approaches to address questions on movement ecology. They can be used to infer the behavioural response of animal species and, also demonstrate the importance of applying such approaches to endangered species conservation within a context of changing humanized scenarios.