bluuebunny/crossref_metadata_2025_split huggingface dataset:
AtMCM10 promotes DNA replication‐coupled nucleosome assembly in Arabidopsis
Authors: Zhao Xinjie , Wang Jingyi , Jin Dan , Cheng Jinkui , Chen Hui , Li Zhen , Wang Yu , Lou Huiqiang , Zhu Jian‐Kang , Du Xuan , Gong Zhizhong 
Published: December 2022

ABSTRACTMinichromosome Maintenance protein 10 (MCM10) is essential for DNA replication initiation and DNA elongation in yeasts and animals. Although the functions of MCM10 in DNA replication and repair have been well documented, the detailed mechanisms for MCM10 in these processes are not well known. Here, we identified AtMCM10 gene through a forward genetic screening for releasing a silenced marker gene. Although plant MCM10 possesses a similar crystal structure as animal MCM10, AtMCM10 is not essential for plant growth or development in Arabidopsis. AtMCM10 can directly bind to histone H3‐H4 and promotes nucleosome assembly in vitro. The nucleosome density is decreased in Atmcm10, and most of the nucleosome density decreased regions in Atmcm10 are also regulated by newly synthesized histone chaperone Chromatin Assembly Factor‐1 (CAF‐1). Loss of both AtMCM10 and CAF‐1 is embryo lethal, indicating that AtMCM10 and CAF‐1 are indispensable for replication‐coupled nucleosome assembly. AtMCM10 interacts with both new and parental histones. Atmcm10 mutants have lower H3.1 abundance and reduced H3K27me1/3 levels with releasing some silenced transposons. We propose that AtMCM10 deposits new and parental histones during nucleosome assembly, maintaining proper epigenetic modifications and genome stability during DNA replication.
Caregivers' perception of adults with Down syndrome willingness to participate in research
Authors: Kyprianou N. , Hendrix J. , Hillerstrom H. , Grimm R. , Kirova A.‐M. , Rubenstein E. 
Published: December 2022

AbstractBackgroundHistorically, individuals with Down syndrome have been excluded from clinical research. Our objective was to assess the degree of interest adults with Down syndrome have in participating in research from the perspective of the caregivers who care for them.MethodsWe conducted an online survey of N = 390 caregivers of adults with Down syndrome and asked about interest in research participation and demographics.ResultsCaregivers were mostly family members, older than 55 years, and White. Caregivers reported that the adult with Down syndrome that they cared for would be more comfortable participating in research that was physiological, such as research involving fit bits (70.2% would participate), exercise (63.3%) or diet apps (53.9%), whereas they would be less likely to participate in clinical trials involving more invasive procedures such as injections (10.9%) and laboratory exams like MRIs (32.0%). We found little difference by age or gender of the adult with Down syndrome or by caregiver education level.ConclusionsOur survey identified high interest for less invasive studies, illustrating acceptability of observational and lifestyle studies. More effort may be needed to understand fear and barriers to participation and to create tools and methods to increase interest in more invasive studies.
Couple therapy with parents: Results from a pragmatic randomized controlled trial testing the Integrative Brief Systemic Intervention (IBSI)
Authors: Darwiche Joëlle , Antonietti Jean‐Philippe , Nunes Cindy Eira , Favez Nicolas , Liekmeier Esther , de Roten Yves 
Published: December 2022

AbstractThis study aimed to compare the effectiveness of the Integrative Brief Systemic Intervention (IBSI), combining therapeutic work on marital and coparenting relationships with brief systemic therapy (BST‐as‐usual) for parent couples. Couples were randomly assigned to the IBSI (n = 51) or BST (n = 50). Both treatments were six‐session interventions and lasted about 6 months. Questionnaires on individual, marital, coparenting and family‐related functioning were completed before and after therapy, and at 6‐month follow‐up. A significant improvement in all areas of functioning was observed after treatment and maintained at follow‐up for both IBSI and BST‐as‐usual treatments. No significant differences in outcomes were found between the treatments. Additionally, women reported more distress overall than men, and this distress was reduced more significantly after therapy. Last, BST‐as‐usual couples requested more additional sessions compared to IBSI couples. This study extends the literature on couple therapy with parents.
Evaluation of molasses on fermentation profiles of wheat straw ensiled with tall fescue in the southwest Tibet
Authors: Dong Dong , Dai Tongtong , Zong Cheng , Yin Xuejing , Bao Yuhong , Shao Tao 
Published: December 2022

AbstractTwo experiments were carried out to determine the optimal proportion of mixed silage made with wheat straw and tall fescue, and further to evaluate the effects of molasses on fermentation quality. In Experiment 1, wheat straw and tall fescue were mixed at proportions of 10:0 (Control), 8:2 (WT20), 6:4 (WT40) and 4:6 (WT60) on fresh weight (FW) basis. Inclusion of tall fescue significantly (p < 0.05) increased lactic acid, water‐soluble carbohydrate contents and ratio of lactic to acetic acid, and significantly (p < 0.05) decreased pH and contents of dry matter, NH3‐N and volatile fatty acids. WT60 had the highest (p < 0.05) lactic acid content, and the lowest (p < 0.05) pH and butyric acid content. In Experiment 2, the mixture of wheat straw and tall fescue (4/6) were treated with 0%, 3%, 4% and 5% molasses on FW basis (defined as control, WTM3, WTM4 and WTM5 respectively). Molasses addition significantly (p < 0.05) increased lactic acid and water‐soluble carbohydrate contents, and significantly (p < 0.05) decreased pH and ammonia‐nitrogen content as compared with control. Acetic acid content slightly (p > 0.05) decreased during ensiling, while trace amounts of propionic and butyric acids were observed. WTM5 had the lowest pH and the highest (p < 0.05) lactic acid, water‐soluble carbohydrate contents and ratio of lactic to acetic acid at end of ensiling. In conclusion, the fermentation quality was maximally improved when the addition rate of molasses was 5% in 40% wheat straw ensiled with 60% tall fescue.
“Teachers think the kids around here, don't really want to learn”: Street‐identified black men and women's attitudes toward teachers and schooling
Authors: Payne Yasser Arafat , Aviles Ann M. , Yates Nefetaria A. 
Published: December 2022

AbstractThis street participatory action research project explored the reflective schooling experiences of street identified Black men and women (ages 18–35) in two small low‐income neighborhoods. Secondary analysis of survey (N = 520) and interview (N = 46) data examined: (1) How are attitudes toward schooling and teachers affected by race, gender and age?; and (2) How do students utilize a street‐identity as a site of resilience inside schools? Overall, street‐identified study participants held positive attitudes toward schooling, but generally performed poorly in schools and had negative experiences with educators. No significance was found as a function of gender and age regarding attitudes toward schooling and attitudes toward teachers. Also, interview results, across gender and age, suggest school‐related structural challenges and poor teacher‐student relationships contributed to severe conflict between students and teachers; and between students. Interviewees argued some Black students internalized a street identity or became disruptive and even engaged in school violence as a protective mechanism to endure hostile schooling environments. Moreover, Street PAR is discussed as a method and intervention to improve student performance and resolve concerns between students and educators.
The impact of rural emptiness on gender relations in postsocialist Albania
Authors: Brooks Sally , Meçe Merita H. 
Published: December 2022

AbstractThis article explores impacts of dramatic levels of outmigration from Puka, a remote region of northern Albania. Since 1991 depopulation, economic decline and state withdrawal have created conditions of what Dzenovska calls rural emptiness. Drawing on focus group discussions with women from eight villages, it explores how conditions of rural emptiness have led to a transformation of gender contracts, with women taking on additional responsibilities. However, these changes have been accommodated within a re‐traditionalised patriarchal system through the devaluation of tasks assigned to women, their increased surveillance by male relatives and erosion of women's social life outside the household.
A preliminary investigation of the impact of artificially carbonated water bathing on canine skin physiological and barrier function
Authors: Iyori Keita , Tamagawa Shinichi , Ide Kaori , Nishifuji Koji 
Published: December 2022

AbstractBackgroundThe effect of carbon dioxide (CO2)‐rich water bathing on the skin has been studied extensively in humans. However, there have been few studies evaluating the impact of CO2‐rich water bathing on canine skin physiology and barrier functions.ObjectivesTo evaluate the impact of artificially carbonated water (ACW) bathing on skin parameters in healthy beagles.AnimalsSix healthy beagles with no history of skin disease.Materials and MethodsBody temperature, skin temperature, transepidermal water loss (TEWL), skin hydration and skin blood flow were evaluated before and after single ACW bathing (37°C, 20 min) with a CO2 concentration of >1000 ppm.ResultsAfter ACW bathing, skin blood flow significantly increased (p < 0.0001), yet there were no significant changes in body temperature (p = 0.3124), skin temperature (p = 0.4911), TEWL (p = 0.5167) or skin hydration (p = 0.3084). There were no adverse events during the trials.Conclusions and Clinical ImportanceArtificially carbonated water water bathing could potentially increase skin blood flow without affecting skin temperature, body temperature and skin barrier function in dogs, similar to its effects in humans.
In vitro elution of amikacin from four hydrogel preparations: A pilot study
Authors: Hottmann Natasha M. , Raines Katherine , Droog Madeline , Steiner Joerg M. , Thieman Mankin Kelley M. 
Published: December 2022

AbstractObjectiveTo determine in vitro elution of amikacin from poloxamer 407 NF, 1% carboxymethylcellulose (CMC), 3% CMC, 5% CMC, and control (sterile water).Study designDescriptive in vitro.Sample populationTriplicate samples from each experimental group.MethodsAmikacin solution was prepared in poloxamer 407 NF, 1% CMC, 3% CMC, 5% CMC, or sterile water. Then, 1 ml of phosphate‐buffered saline (PBS) was added to each of three aliquots per base and the samples were incubated at 37°C. PBS was removed and replaced at 1, 4, 8, 12, 24, 48, 72, 96, 120, 144, 168, 192, 216, and 240 hours and amikacin concentration was measured.ResultsThe highest median concentration of amikacin in the eluent of poloxamer 407 NF, 3% CMC, and 5% CMC was observed at 48 hours: 3300, 3030, and 2190 μg/ml, respectively. The highest median concentration of amikacin in the eluent of 1% CMC and sterile water were observed at 1 hour: 13300 and 15600 μg/ml, respectively. Median eluent concentration of amikacin exceeded 2000 μg/ml (the reported minimum inhibitory concentration [MIC] of certain biofilm‐producing methicillin‐resistant Staphylococcus pseudintermedius) from 24 to 96 hours for poloxamer 407 NF, 24–72 hours for 3% CMC, 48–72 hours for 5% CMC, 1–4 hours for 1% CMC, and 1–4 hours for sterile water.ConclusionAmikacin elution from tested substances reached or exceeded target MIC during the 240 hours tested.Clinical significanceHydrogel‐amikacin solutions may be useful topical treatment options for some infected wounds. In vivo safety and efficacy should be evaluated.
Maternal allogeneic cancellous bone graft for the treatment of osteitis along the physeal scar of the proximal metatarsus in a foal
Authors: Sauer Fay J. , Verhaar Nicole , Geburek Florian 
Published: December 2022

AbstractObjectiveTo describe the treatment and outcome of a foal with a fresh allogenic cancellous bone graft after surgical debridement of a traumatic septic osteitis.AnimalA neonatal Quarter Horse foal.Study designCase report.MethodsThe foal sustained a traumatic laceration exposing the proximal third metatarsal bone. One week after surgical debridement and closure, radiographic signs of septic osteitis were noted along the physeal scar. The lesion was debrided, and antimicrobial therapy was implemented. The infection resolved but left a large defect in the metaphysis and epiphysis. Grafting was indicated to avoid pathologic fractures of the plantar and proximal cortices. Due to a discrepancy between defect size and the bone stock of the foal, an allogeneic cancellous bone graft was harvested from the dam's tuber coxae and used to fill the foal's defect.ResultsNo adverse reactions to the graft were noted. After 1 month, the wound had healed. Radiographic examination was consistent with graft incorporation in the bone structure. The foal was sound at a walk and trot when examined at 6, 12, and 21 months. The bone's contour was even and its structure homogeneously radio dense. The surgical site of the mare healed without complications.ConclusionFresh allogenic cancellous bone grafting resulted in the healing of a large traumatic‐septic bone defect in a foal, with an excellent functional and cosmetic outcome. For future use, compatibility testing should be considered prior to allogeneic bone grafting.
Thermodynamic Properties of Vapors
Authors: Leib Erich F. 
Published: December 2022

Abstract
By introducing new properties of state and an ideal system, that is, a perfect vapor, the representation of the thermodynamic properties of vapors is simplified. The field of state reduces to one curve of state which requires in the experimental investigation of vapors only as many measurements as are necessary for the determination of this curve. The equations of state are found more easily, the calculations required to determine the vapor tables are reduced, and a simple formula for the external work is found. An analytic investigation of the equilibrium of a liquid with its vapor shows how the vapor-pressure curve can be found from the equation of state.
Interpretation of Creep Tests on Tubes
Authors: Soderberg C. R. 
Published: December 2022

Abstract
In a companion paper, Professor Norton has presented the results of creep tests on tubes, carried out under Project 10, of the Joint Research Committee on Effect of Temperature on the Properties of Metals. The present paper contains a discussion of the phenomena involved in the light of the accepted theory of yielding, the theoretical basis for the present research program, and the significance of the results thus far obtained.
Measurement of Inertial and Acoustic Waves in a Turbine Chute Rim Seal Cavity
Authors: Bru Revert Anna , Beard Paul F. , Chew John W. 
Published: December 2022

AbstractA chute rim seal cavity has been instrumented with time-resolved and time-averaged pressure transducers as well as a gas concentration measurement system at the Oxford Rotor Facility. The unsteady pressure transducers were unevenly distributed along the circumference generating 15 combinations of angular spacing to conduct a phase analysis. The test operating conditions were modified through different configurations of mainstream flow, a range of rotational speeds and several rates of purge flow to obtain test data in the regimes of rotationally-driven, pressure-driven, and combined ingestion. The rotating unsteady flow structures within the rim seal cavity were studied through a combination of phase analysis of dynamic pressure signals and inspection of the frequency domain. The introduction of mainstream flow (axial and swirled) increased the overall unsteadiness exciting an additional band of lower frequencies. The phase analysis revealed these were associated with acoustic waves, providing the first experimental evidence of the presence and coexistence of inertial and acoustic waves in the rim seal cavity. The interaction of the inertial and acoustic waves strongly depends on the operating conditions, and may be linked to changes in sealing effectiveness.
How to Build Your Mentoring Tree—Insight Gained From a 36-Year Career in Biomedical Engineering
Authors: Grimm Michele J. 
Published: December 2022

Abstract
Mentoring is often viewed as a unidirectional relationship, with the senior, more seasoned individual imparting knowledge, guidance, and experience to more junior individuals. But this structure limits our ability to recognize that the mentoring relationship can bring benefits and opportunities for growth to the mentor as well. On the occasion of receiving the 2022 Robert M. Nerem Education and Mentorship Medal, I have had the opportunity to reflect on the mentoring that I have been lucky enough to participate in—both as mentor and mentee—during my academic career. This paper discusses some of those insights and presents the concept of a mentoring tree—through which we can identify multiple mentors and mentees, each of whom can provide mutual support and insight as we progress through our careers. Each individual who is part of our mentoring tree can play a role at different times and with different challenges within our professional path. This everchanging and growing structure provides continuous mentoring without overtaxing any single relationship.
Ontology of the complex rare-earth elements mineral system
Authors: Babaie Hassan A. , Davarpanah Armita , Elliott W. Crawford 
Published: December 2022

ABSTRACTThe ree-ont ontology formally represents the semantics of the complex interactions in the rare-earth elements (REE) mineral system from a self-organized criticality perspective. This ontology models various primarily heat-driven, melt-led, ore-forming processes that contribute to the formation of the REE-bearing alkaline intrusive bodies and related hydrothermal deposits. It also models the processes that form sediment-hosted concentrations of the REE from the weathering and erosion of the primary REE deposits. The ontology explicitly formalizes the interactions among the members of various magmatic, hydrothermal, basinal, regolith, and supergene subsystems. It represents the principal geodynamic, tectonic, and geological driver, trigger, carrier, conduit, and barrier roles that the components of the REE mineral system recursively play in enriching the REE during the subcritical and critical stages of the evolution of the complex system in and on the surface of the Earth’s crust. ree-ont establishes a logical machine processable basis for understanding the dynamic interactions among various components of the REE mineral system by extending the upper-level Basic Formal Ontology (BFO) and its underlying mid-level Common Core Ontologies (CCO).
Digital solution for detection of undiagnosed diabetes using machine learning-based retinal image analysis
Authors: Zee Benny , Lee Jack , Lai Maria , Chee Peter , Rafferty James , Thomas Rebecca , Owens David 
Published: December 2022

IntroductionUndiagnosed diabetes is a global health issue. Previous studies have estimated that about 24.1%–75.1% of all diabetes cases are undiagnosed, leading to more diabetic complications and inducing huge healthcare costs. Many current methods for diabetes diagnosis rely on metabolic indices and are subject to considerable variability. In contrast, a digital approach based on retinal image represents a stable marker of overall glycemic status.Research design and methodsOur study involves 2221 subjects for developing a classification model, with 945 subjects with diabetes and 1276 controls. The training data included 70% and the testing data 30% of the subjects. All subjects had their retinal images taken using a non-mydriatic fundus camera. Two separate data sets were used for external validation. The Hong Kong testing data contain 734 controls without diabetes and 660 subjects with diabetes, and the UK testing data have 1682 subjects with diabetes.ResultsThe 10-fold cross-validation using the support vector machine approach has a sensitivity of 92% and a specificity of 96.2%. The separate testing data from Hong Kong provided a sensitivity of 99.5% and a specificity of 91.1%. For the UK testing data, the sensitivity is 98.0%. The accuracy of the Caucasian retinal images is comparable with that of the Asian data. It implies that the digital method can be applied globally. Those with diabetes complications in both Hong Kong and UK data have a higher probability of risk of diabetes compared with diabetes subjects without complications.ConclusionsA digital machine learning-based method to estimate the risk of diabetes based on retinal images has been developed and validated using both Asian and Caucasian data. Retinal image analysis is a fast, convenient, and non-invasive technique for community health applications. In addition, it is an ideal solution for undiagnosed diabetes prescreening.
Progressive resistance training in young people with Prader-Willi syndrome: protocol for a randomised trial (PRESTO)
Authors: Shields Nora , Bennell Kim L , Southby Alesha , Rice Lauren J , Markovic Tania , Bigby Christine , Prendergast Luke , Watts Jennifer J , Schofield Cara , Loughnan Georgina , Franklin Janet , Levitt David , Chikani Viral , McCallum Zoe , Blair Susan , Proietto Joseph , Taylor Nicholas F 
Published: December 2022

IntroductionPreliminary evidence suggests that progressive resistance training may be beneficial for people with Prader-Willi Syndrome (PWS), a rare genetic condition that results in muscle weakness and low muscle tone.To establish whether community-based progressive resistance training is effective in improving the muscle strength of people with PWS; to determine cost-effectiveness; and, to complete a process evaluation assessing intervention fidelity, exploring mechanisms of impact, understanding participant experiences and identifying contextual factors affecting implementation.Methods and analysisA multisite, randomised controlled trial will be completed. Sixty participants with PWS will be randomised to receive either progressive resistance training (experimental) or non-progressive exercise (placebo control). Participants will be aged 13 to 60 years, be able to follow simple instructions in English and have no contraindications to performing progressive resistance training. The experimental group will complete progressive resistance training two times weekly for 24 weeks supervised by an exercise professional at a community gym. The control group will receive all aspects of the intervention except progressive overload. Outcomes will be assessed at week 25 (primary endpoint) and week 52 by a blinded assessor. The primary outcome is muscle strength assessed using one repetition maximum for upper limb and lower limb. Secondary outcomes are muscle mass, functional strength, physical activity, community participation, health-related quality of life and behaviour. Health economic analysis will evaluate cost-effectiveness. Process evaluation will assess safety and intervention fidelity, investigate mechanism of impact, explore participant experiences and identify contextual factors affecting implementation. Data collection commenced in February 2020 and will conclude in September 2023.Ethics and disseminationEthical approval was obtained from The Royal Children’s Hospital Human Research Ethics Committee (HREC/50874/RCHM-2019) under the National Mutual Acceptance initiative. Research governance approvals were obtained from five clinical sites. Results will be disseminated through published manuscripts, conference presentations, public seminars and practical resources for stakeholder groups.Trial registration numberACTRN12620000416998; Australian and New Zealand Clinical Trial Registry.