bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRPM7 kinase mediates hypomagnesemia-induced seizure-related death
Authors: Liu Man , Liu Hong , Feng Feng , Krook-Magnuson Esther , Dudley Samuel C. 
Published: May 2023

AbstractHypomagnesemia (HypoMg) can cause seizures and death, but the mechanism is unknown. Transient receptor potential cation channel subfamily M 7 (TRPM7) is a Mg transporter with both channel and kinase function. In this study, we focused on the kinase role of TRPM7 in HypoMg-induced seizures and death. Wild type C57BL/6J mice and transgenic mice with a global homozygous mutation in the TRPM7 kinase domain (TRPM7K1646R, with no kinase function) were fed with control diet or a HypoMg diet. After 6 weeks of HypoMg diet, mice had significantly decreased serum Mg, elevated brain TRPM7, and a significant rate of death, with females being most susceptible. Deaths were immediately preceded by seizure events. TRPM7K1646R mice showed resistance to seizure-induced death. HypoMg-induced brain inflammation and oxidative stress were suppressed by TRPM7K1646R. Compared to their male counterparts, HypoMg female mice had higher levels of inflammation and oxidative stress in the hippocampus. We concluded that TRPM7 kinase function contributes seizure-induced deaths in HypoMg mice and that inhibiting the kinase reduced inflammation and oxidative stress.
Erosion-inhibiting potential of the stannous fluoride-enriched CPP-ACP complex in vitro
Authors: Al Saady Deena , Hall Colin , Edwards Suzanne , Reynolds Eric C. , Richards Lindsay C. , Ranjitkar Sarbin 
Published: May 2023

AbstractCurrently available anti-erosive agents only provide partial protection, emphasizing the need to enhance their performance. By characterizing erosive enamel wear at the nanoscale, the aim of this in vitro study was to assess the anti-erosive effects of SnF2 and CPP-ACP both individually and synergistically. Erosion depths were assessed longitudinally on 40 polished human enamel specimens after 1, 5, and 10 erosion cycles. Each cycle comprised one-min erosion in citric acid (pH 3.0) and one-min treatment in whole saliva (control group) or a slurry of one of the three anti-erosive pastes (10% CPP-ACP; 0.45% SnF2 (1100 ppm F); or SnF2/CPP-ACP (10% CPP-ACP + 0.45% SnF2)) (n = 10 per group). Scratch depths were assessed longitudinally in separate experiments using a similar protocol after 1, 5, and 10 cycles. Compared with the control groups, all slurries reduced erosion depths after 1 cycle (p ≤ 0.004) and scratch depths after 5 cycles (p ≤ 0.012). The order of anti-erosive potential was SnF2/CPP-ACP > SnF2 > CPP-ACP > control for erosion depth analysis, and SnF2/CPP-ACP > (SnF2 = CPP-ACP) > control for scratch depth analysis. These data provide ‘proof of concept’ evidence that SnF2/CPP-ACP has superior anti-erosive potential compared to SnF2 or CPP-ACP alone.
Apoptosis induction in cancer cell lines and anti-inflammatory and anti-pathogenic properties of proteinaceous metabolites secreted from potential probiotic Enterococcus faecalis KUMS-T48
Authors: Salek Faezeh , Mirzaei Hamid , Khandaghi Jalil , Javadi Afshin , Nami Yousef 
Published: May 2023

AbstractPotential probioticEnterococcus faecalisKUMS-T48, isolated from a kind of Iranian traditional dairy product (Tarkhineh), was assessed for its anti-pathogenic, anti-inflammatory and anti-proliferative properties against HT-29 and AGS cancer cell lines. This strain showed strong effects onBacillus subtilisandListeria monocytogenesand moderate effect onYersinia enterocolitica, while indicated weak effect onKlebsiella pneumoniaeandEscherichia coli. Also, neutralizing the cell-free supernatant and treating it with catalase and proteinase K enzymes reduced the antibacterial effects. Similar to Taxol, the cell-free supernatant ofE. faecalisKUMS-T48 inhibited the in vitro proliferation of both cancer cells in a dose-dependent manner, but unlike Taxol, they had no activity against normal cell line (FHs-74). Pronase-treatment of the CFS ofE. faecalisKUMS-T48 abrogated its anti-proliferative capacity, thereby showing the proteinaceous nature of the cell-free supernatant. Further, induction of apoptosis-based cytotoxic mechanism byE. faecalisKUMS-T48 cell-free supernatant is related to anti-apoptotic genes ErbB-2 and ErbB-3, which is different from Taxol’s apoptosis induction (intrinsic mitochondria apoptosis pathway). Also, as evidenced by a decline in interleukin 1β inflammation-promoting gene expression and a rise in the anti-inflammatory interleukin-10 gene expression in the HT-29 cell line, probioticE. faecalisKUMS-T48 cell-free supernatant demonstrated a significant anti-inflammatory impact.
DNA methylation variations and epigenetic aging in telomere biology disorders
Authors: Carlund Olivia , Norberg Anna , Osterman Pia , Landfors Mattias , Degerman Sofie , Hultdin Magnus 
Published: May 2023

AbstractTelomere Biology Disorders (TBDs) are characterized by mutations in telomere-related genes leading to short telomeres and premature aging but with no strict correlation between telomere length and disease severity. Epigenetic alterations are also markers of aging and we aimed to evaluate whether DNA methylation (DNAm) could be part of the pathogenesis of TBDs. In blood from 35 TBD cases, genome-wide DNAm were analyzed and the cases were grouped based on relative telomere length (RTL): short (S), with RTL close to normal controls, and extremely short (ES). TBD cases had increased epigenetic age and DNAm alterations were most prominent in the ES-RTL group. Thus, the differentially methylated (DM) CpG sites could be markers of short telomeres but could also be one of the mechanisms contributing to disease phenotype since DNAm alterations were observed in symptomatic, but not asymptomatic, cases with S-RTL. Furthermore, two or more DM-CpGs were identified in four genes previously linked to TBD or telomere length (PRDM8, SMC4, VARS, and WNT6) and in three genes that were novel in telomere biology (MAS1L, NAV2, and TM4FS1). The DM-CpGs in these genes could be markers of aging in hematological cells, but they could also be of relevance for the progression of TBD.
Cost-effective Whole Exome Sequencing discovers pathogenic variant causing Neurofibromatosis type 1 in a family from Jammu and Kashmir, India
Authors: Spolia Akshi , Angural Arshia , Sharma Varun , Shipra  , Razdan Sushil , Dhar Manoj K. , Mahajan Ankit , Verma Vijeshwar , Pandita Kamal K. , Sharma Swarkar , Rai Ekta 
Published: May 2023

AbstractNeurofibromatosis type 1 (NF1) is a multisystemic hereditary disorder associated with an increased risk of benign and malignant tumor formation predominantly on the skin, bone, and peripheral nervous system. It has been reported that out of all the NF1 cases, more than 95% cases develop the disease due to heterozygous loss-of-function variants in Neurofibromin (NF1) gene. However, identification of NF1 causative variants by presently recommended method of gene-targeted Sanger sequencing is challenging and cost-intensive due to the large size of the NF1gene with 60 exons spanning about 350 kb. Further, conducting the genetic studies is difficult in low resource regions and among families with the limited financial capabilities, restricting them from availing diagnostic as well as proper disease management measures. Here, we studied a three-generation family from Jammu and Kashmir state in India, with multiple affected family members showing clinical indications of NF1. We combinedly used two applications, Whole Exome Sequencing (WES) and Sanger sequencing, for this study and discovered a nonsense variant NM_000267.3:c.2041C>T (NP_000258.1:p.Arg681Ter*) in exon 18 of NF1 gene in a cost effective manner. In silico analyses further substantiated the pathogenicity of this novel variant. The study also emphasized on the role of Next Generation Sequencing (NGS) as a cost-effective method for the discovery of pathogenic variants in disorders with known phenotypes found in large sized candidate genes. The current study is the first study based on the genetic characterization of NF1 from Jammu and Kashmir–India, highlighting the importance of the described methodology adopted for the identification and understanding of the disease in low resource region. The early diagnosis of genetic disorders would open the door to appropriate genetic counseling, reducing the disease burden in the affected families and the general population at large.
Changes in the chemical compositions and biological properties of kombucha beverages made from black teas and pineapple peels and cores
Authors: Phung Ly Tu , Kitwetcharoen Haruthairat , Chamnipa Nuttaporn , Boonchot Nongluck , Thanonkeo Sudarat , Tippayawat Patcharaporn , Klanrit Preekamol , Yamada Mamoru , Thanonkeo Pornthap 
Published: May 2023

AbstractSeveral raw materials have been used as partial supplements or entire replacements for the main ingredients of kombucha to improve the biological properties of the resulting kombucha beverage. This study used pineapple peels and cores (PPC), byproducts of pineapple processing, as alternative raw materials instead of sugar for kombucha production. Kombuchas were produced from fusions of black tea and PPC at different ratios, and their chemical profiles and biological properties, including antioxidant and antimicrobial activities, were determined and compared with the control kombucha without PPC supplementation. The results showed that PPC contained high amounts of beneficial substances, including sugars, polyphenols, organic acids, vitamins, and minerals. An analysis of the microbial community in a kombucha SCOBY (Symbiotic Cultures of Bacteria and Yeasts) using next-generation sequencing revealed that Acetobacter and Komagataeibacter were the most predominant acetic acid bacteria. Furthermore, Dekkera and Bacillus were also the prominent yeast and bacteria in the kombucha SCOBY. A comparative analysis was performed for kombucha products fermented using black tea and a fusion of black tea and PPC, and the results revealed that the kombucha made from the black tea and PPC infusion exhibited a higher total phenolic content and antioxidant activity than the control kombucha. The antimicrobial properties of the kombucha products made from black tea and the PPC infusion were also greater than those of the control. Several volatile compounds that contributed to the flavor, aroma, and beneficial health properties, such as esters, carboxylic acids, phenols, alcohols, aldehydes, and ketones, were detected in kombucha products made from a fusion of black tea and PPC. This study shows that PPC exhibits high potential as a supplement to the raw material infusion used with black tea for functional kombucha production.
Impact of body mass index on outcomes after lumbar spine surgery
Authors: Nakajima Koji , Miyahara Junya , Ohtomo Nozomu , Nagata Kosei , Kato So , Doi Toru , Matsubayashi Yoshitaka , Taniguchi Yuki , Kawamura Naohiro , Higashikawa Akiro , Takeshita Yujiro , Fukushima Masayoshi , Ono Takashi , Hara Nobuhiro , Azuma Seiichi , Iwai Hiroki , Oshina Masahito , Sugita Shurei , Hirai Shima , Masuda Kazuhiro , Tanaka Sakae , Oshima Yasushi 
Published: May 2023

AbstractThe impact of body mass index (BMI) on outcomes after lumbar spine surgery is currently unknown. Previous studies have reported conflicting evidence for patients with high BMI, while little research has been conducted on outcomes for underweight patients. This study aims to examine the impact of BMI on outcomes after lumbar spine surgery. This prospective cohort study enrolled 5622 patients; of which, 194, 5027, and 401 were in the low (< 18.5 kg/m2), normal (18.5–30), and high (≥ 30) BMI groups, respectively. Pain was assessed via the numerical pain rating scale (NPRS) for the lower back, buttock, leg, and plantar area. Quality of life was assessed via the EuroQol 5 Dimension (EQ-5D) and Oswestry Disability Index (ODI). Inverse probability weighting with propensity scores was used to adjust patient demographics and clinical characteristics between the groups. After adjustment, the 1-year postoperative scores differed significantly between groups in terms of leg pain. The proportion of patients who achieved a 50% decrease in postoperative NPRS score for leg pain was also significantly different. Obese patients reported less improvement in leg pain after lumbar spine surgery. The outcomes of patients with low BMI were not inferior to those of patients with normal BMI.
Association of alcohol use and multimorbidity among adults aged 40 years and above in rural South Africa
Authors: Mpinganjira Mafuno G. , Chirwa Tobias , Kabudula Chodziwadziwa. W. , Gómez-Olivé Francesc Xavier , Tollman Stephen , Francis Joel Msafiri 
Published: May 2023

AbstractWe assessed the prevalence of reported alcohol use and its association with multimorbidity among adults aged 40 years and above in a rural, transitioning South African setting. Findings could potentially inform alcohol interventions integration in the prevention and treatment of chronic conditions. We analysed data from the first wave of The Health and Ageing in Africa—a longitudinal Study in an INDEPTH community (HAALSI) nested within the Agincourt Health and Demographic Surveillance Systems, conducted between November 2014 and November 2015 (n = 5059). We computed descriptive statistics and performed univariate analysis to determine factors independently associated with multimorbidity. Age, Body Mass Index, education, sex, and household wealth status and variables with a p-value < 0.20 in univariate analysis were included in multivariable Modified Poisson regression models. Any factors with a p-value of < 0.05 in the final models were considered statistically significant. The first wave of HAALSI was completed by 5059 participants aged 40 years and above and included 2714 (53.6%) females. The prevalence of reported ever alcohol use was 44.6% (n = 2253) and of these 51.9% (n = 1171) reported alcohol use in the last 30 days. The prevalence of HIV multimorbidity was 59.6% (3014/5059) and for multimorbidity without HIV 52.5% (2657/5059). Alcohol use was associated with HIV multimorbidity among all participants (RR: 1.05, 95% CI: 1.02–1.08), and separately for males (RR: 1.05, 95% CI: 1.00–1.10) and females (RR: 1.06, 95%CI: 1.02–1.11). Similarly, alcohol use was associated with multimorbidity without HIV among all participants (RR: 1.05, 95% CI: 1.02–1.09), and separately for males (RR: 1.06, 95% CI: 1.00–1.12) and females (RR: 1.06, 95% CI: 1.01–1.11). Reported alcohol use was common and associated with HIV multimorbidity and multimorbidity without HIV among older adults in rural northeast South Africa. There is a need to integrate Screening, Brief Interventions, and Referral for alcohol Treatment in the existing prevention and treatment of multimorbidity in South Africa.
Humic lakes with inefficient and efficient transfer of matter in planktonic food webs
Authors: Karpowicz Maciej , Grabowska Magdalena , Ejsmont-Karabin Jolanta , Ochocka Agnieszka 
Published: May 2023

AbstractHumic compounds and related factors are the main constraints for the development of zooplankton in humic lakes, leading to low transfer efficiency in food webs. The results of this study indicated that some zooplankton species could have an advantage under these conditions. We found that the mass development of omnivorous Asplanchna priodonta in temperate humic lakes could be caused by the domination of high nutritional algae such as Gonyostomum semen and Botryococcus braunii. These algae are too large for most zooplankton to ingest, but A. priodonta can feed on a wide range of particles and benefit from this high-nutritional food. Small cladocerans (Ceriodaphnia, Bosmina) might be favored when picoplankton and small algae-dominate humic lakes. Therefore, some zooplankton species could have an advantage and control the development of phytoplankton, leading to the effective transfer of matter and energy in the planktonic food web in humic lakes.
Analysis of spatial and temporal trend of hydro-climatic parameters in the Kilombero River Catchment, Tanzania
Authors: Sigalla Onesmo Zakaria , Valimba Patrick , Selemani Juma Rajabu , Kashaigili Japhet J. , Tumbo Madaka 
Published: May 2023

AbstractInadequate knowledge on actual water availability, have raised social-economic conflicts that necessitate proper water management. This requires a better understanding of spatial–temporal trends of hydro-climatic variables as the main contributor to available water for use by sectors of economy. The study has analysed the trend of hydro-climatic variables viz. precipitation, temperature, evapotranspiration and river discharge. One downstream river gauge station was used for discharge data whereas a total of 9 daily observed and 29 grided satellite stations were used for climate data. Climate Hazards Group InfraRed Precipitation was used for precipitation data and Observational-Reanalysis Hybrid was used for Temperature data. Mann–Kendall Statistical test, Sen’s slope estimator and ArcMap Inverse Distance Weighted Interpolation functionality were employed for temporal, magnitude and spatial trend analysis respectively. Results confirmed that, spatially, there are three main climatic zones in the study area viz. Udzungwa escarpment, Kilombero valley and Mahenge escarpment. On temporal analysis, with exception of the declining potential evapotranspiration trend, all other variables are on increase. This is with catchment rates of 2.08 mm/year, 0.05 °C/year, 0.02 °C/year, 498.6 m3/s/year and − 2.27 mm/year for precipitation, Tmax, Tmin, river discharge and PET respectively. Furthermore, rainfalls start late by a month (November) while temperatures picks earlier by September and October for Tmax and Tmin respectively. Water availability matches farming season. However, it is recommended to improve water resources management practices to limit flow impairment as expansions in sectors of economy are expected. Furthermore, landuse change analysis is recommended to ascertain actual trend and hence future water uptake.
Cutaneous body image in patients with hidradenitis suppurativa: a hospital-based cross-sectional study
Authors: Koumaki Dimitra , Rovithi Evangelia , Solia Apokidou Erato , Papadakis Marios , Katoulis Alexander , Evangelou George 
Published: May 2023

AbstractHidradenitis suppurativa (HS) has been linked with body image (BI) impairment and reduced quality of life (QoL). We sought to evaluate the associations between Cutaneous Body Image Scale (CBIS) and disease severity in HS patients.Between July 2020 and January 2022, a cross-sectional study was carried out including consecutive HS patients above the age of 16 who attended a Tertiary Referral Hospital in Greece. Disease Severity was graded with the Hurley stage, HS-Physician’s Global Assessment (HS-PGA) scale, and the Modified Sartorius scale (MSS). Patients completed at their first visit ten survey instruments including Patients’ Severity of disease, pain and pruritus scale, CBIS, Multidimensional Body-Self Relations Questionnaire (MBSRQ) including 5 subscales: Appearance Evaluation (AE), Appearance Orientation (AO), Body Areas Satisfaction Scale (BASS), Overweight Preoccupation (OWP), and Self Classified Weight (SCW) , Dermatology Quality of Life Index (DLQI), Skindex-16, EQ5D 5L, EQ- visual analogue scale (VAS), PHQ9, and GAD7. In total, 70 HS patients above 16 years old participated, mean [SD] age, 34.44 [11.64] years; 49/70 (70%) males and 21/70 (30%) females. Mean ± SD CBI, DLQI, Skindex-16 total, EQ-5D-5L, EQ VAS, PHQ9 and GAD7 were 5.59 ± 1.58, 11.70 ± 8.88, 52.90 ± 27.75, 0.75 ± 0.21, 62.48 ± 21.12, 7.64 ± 5.56, 7.87 ± 5.23 respectively. Moderate to severe CBI dissatisfaction was reported by 36/70 (51.42%) patients. CBI was correlated with appearance evaluation (AE) (p < 0.01, r = 0.544), body areas satisfaction (BASS) (p < 0.01, r = 0.481), and overweight preoccupation subscale (OWPS) (p < 0.01, r =  − 0.267), and Skindex-16 (p < 0.01, r =  − 0.288). HS patients with affected genital areas scored higher in disease patient’s severity score (p = 0.015), and male patients scored higher in Skindex-16 than females(p < 0.01). Our study found that the mean of CBI in HS patients was 5.59 ± SD 1.58. Predictors for CBI dissatisfaction were low scores of MBSRQ Appearance Evaluation (AE) and Body Areas Satisfaction Subscale (BASS).
Ultrafine aramid nanofibers prepared by high-efficiency wet ball-milling-assisted deprotonation for high-performance nanopaper
Authors: Han Gaojie , Zhou Bing , Li Zhaoyang , Feng Yuezhan , Liu Chuntai , Shen Changyu 
Published: May 2023

Wet ball milling-assisted deprotonation (BMAD) strategy was put forward and carried out to prepare aramid nanofiber (ANF) with high preparation efficiency (20 g L−1 h−1), ultrafine fiber diameter (2.09 nm) and narrow distribution (1.0-4.0 nm).
The contribution of binary star formation via core fragmentation on protostellar multiplicity
Authors: Kuruwita Rajika L. , Haugbølle Troels 
Published: May 2023

Context. Observations of young multiple star systems find a bimodal distribution in companion frequency and separation. The origin of these peaks has often been attributed to binary formation via core and disc fragmentation. However, theory and simulations suggest that young stellar systems that form via core fragmentation undergo significant orbital evolution.
Aims. We investigate the influence of the environment on the formation and orbital evolution of multiple star systems, and how core fragmentation contributes to the formation of close (20 − 100 AU) binaries. We use multiple simulations of star formation in giant molecular clouds and compare them to the multiplicity statistics of the Perseus star-forming region.
Methods. Simulations were run with the adaptive mesh refinement code RAMSES with sufficient resolution to resolve core fragmentation beyond 400 AU and dynamical evolution down to 16.6 AU, but without the possibility of resolving disc fragmentation. The evolution of the resulting stellar systems was followed over millions of years.
Results. We find that star formation in lower gas density environments is more clustered; however, despite this, the fractions of systems that form via dynamical capture and core fragmentation are broadly consistent at ∼40% and ∼60%, respectively. In all gas density environments, we find that the typical scale at which systems form via core fragmentation is 103 − 3.5 AU. After formation, we find that systems that form via core fragmentation have slightly lower inspiral rates (∼10−1.68 AU yr−1 measured over the first 10 000 yr) compared to dynamical capture (∼10−1.32 AU yr−1). We then compared the simulation with the conditions most similar to the Perseus star-forming region to determine whether the observed bimodal distribution can be replicated. We find that it can be replicated, but it is sensitive to the evolutionary state of the simulation.
Conclusions. Our results indicate that a significant number of low-mass close binaries with separations from 20 − 100 AU can be produced via core fragmentation or dynamical capture due to efficient inspiral, without the need for a further contribution from disc fragmentation.