bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural activation of regions involved in food reward and cognitive control in young females with anorexia nervosa and atypical anorexia nervosa versus healthy controls
Authors: Eddy Kamryn T. , Plessow Franziska , Breithaupt Lauren , Becker Kendra R. , Slattery Meghan , Mancuso Christopher J. , Izquierdo Alyssa M. , Van De Water Avery L. , Kahn Danielle L. , Dreier Melissa J. , Ebrahimi Seda , Deckersbach Thilo , Thomas Jennifer J. , Holsen Laura M. , Misra Madhusmita , Lawson Elizabeth A. 
Published: June 2023

AbstractAnorexia nervosa (AN) and atypical AN (AtypAN) are complex neurobiological illnesses that typically onset in adolescence with an often treatment-refractory and chronic illness trajectory. Aberrant eating behaviors in this population have been linked to abnormalities in food reward and cognitive control, but prior studies have not examined respective contributions of clinical characteristics and metabolic state. Research is needed to identify specific disruptions and inform novel intervention targets to improve outcomes. Fifty-nine females with AN (n = 34) or AtypAN (n = 25), ages 10–22 years, all ≤90% expected body weight, and 34 age-matched healthy controls (HC) completed a well-established neuroimaging food cue paradigm fasting and after a standardized meal, and we used ANCOVA models to investigate main and interaction effects of Group and Appetitive State on blood oxygenation level-dependent (BOLD) activation for the contrast of exposure to high-calorie food images minus objects. We found main effects of Group with greater BOLD activation in the dorsal anterior cingulate cortex (dACC), dorsolateral prefrontal cortex (DLPFC), hippocampus, caudate, and putamen for AN/AtypAN versus HC groups, and in the three-group model including AN, AtypAN, and HC (sub-)groups, where differences were primarily driven by greater activation in the AtypAN subgroup versus HC group. We found a main effect of Appetitive State with increased premeal BOLD activation in the hypothalamus, amygdala, nucleus accumbens, and caudate for models that included AN/AtypAN and HC groups, and in BOLD activation in the nucleus accumbens for the model that included AN, AtypAN, and HC (sub-)groups. There were no interaction effects of Group with Appetitive State for any of the models. Our findings demonstrate robust feeding-state independent group effects reflecting greater neural activation of specific regions typically associated with reward and cognitive control processing across AN and AtypAN relative to healthy individuals in this food cue paradigm. Differential activation of specific brain regions in response to the passive viewing of high-calorie food images may underlie restrictive eating behavior in this clinical population.
Histone demethylases in the regulation of immunity and inflammation
Authors: Qu Lihua , Yin Tong , Zhao Yijin , Lv Wenting , Liu Ziqi , Chen Chao , Liu Kejun , Shan Shigang , Zhou Rui , Li Xiaoqing , Dong Huifen 
Published: June 2023

AbstractPathogens or danger signals trigger the immune response. Moderate immune response activation removes pathogens and avoids excessive inflammation and tissue damage. Histone demethylases (KDMs) regulate gene expression and play essential roles in numerous physiological processes by removing methyl groups from lysine residues on target proteins. Abnormal expression of KDMs is closely associated with the pathogenesis of various inflammatory diseases such as liver fibrosis, lung injury, and autoimmune diseases. Despite becoming exciting targets for diagnosing and treating these diseases, the role of these enzymes in the regulation of immune and inflammatory response is still unclear. Here, we review the underlying mechanisms through which KDMs regulate immune-related pathways and inflammatory responses. In addition, we also discuss the future applications of KDMs inhibitors in immune and inflammatory diseases.
Mitochondrial matrix protein LETMD1 maintains thermogenic capacity of brown adipose tissue in male mice
Authors: Park Anna , Kim Kwang-eun , Park Isaac , Lee Sang Heon , Park Kun-Young , Jung Minkyo , Li Xiaoxu , Sleiman Maroun Bou , Lee Su Jeong , Kim Dae-Soo , Kim Jaehoon , Lim Dae-Sik , Woo Eui-Jeon , Lee Eun Woo , Han Baek Soo , Oh Kyoung-Jin , Lee Sang Chul , Auwerx Johan , Mun Ji Young , Rhee Hyun-Woo , Kim Won Kon , Bae Kwang-Hee , Suh Jae Myoung 
Published: June 2023

AbstractBrown adipose tissue (BAT) has abundant mitochondria with the unique capability of generating heat via uncoupled respiration. Mitochondrial uncoupling protein 1 (UCP1) is activated in BAT during cold stress and dissipates mitochondrial proton motive force generated by the electron transport chain to generate heat. However, other mitochondrial factors required for brown adipocyte respiration and thermogenesis under cold stress are largely unknown. Here, we show LETM1 domain-containing protein 1 (LETMD1) is a BAT-enriched and cold-induced protein required for cold-stimulated respiration and thermogenesis of BAT. Proximity labeling studies reveal that LETMD1 is a mitochondrial matrix protein. Letmd1 knockout male mice display aberrant BAT mitochondria and fail to carry out adaptive thermogenesis under cold stress. Letmd1 knockout BAT is deficient in oxidative phosphorylation (OXPHOS) complex proteins and has impaired mitochondrial respiration. In addition, BAT-specific Letmd1 deficient mice exhibit phenotypes identical to those observed in Letmd1 knockout mice. Collectively, we demonstrate that the BAT-enriched mitochondrial matrix protein LETMD1 plays a tissue-autonomous role that is essential for BAT mitochondrial function and thermogenesis.
Intracellular pH dynamics regulates intestinal stem cell lineage specification
Authors: Liu Yi , Reyes Efren , Castillo-Azofeifa David , Klein Ophir D. , Nystul Todd , Barber Diane L. 
Published: June 2023

AbstractIntracellular pH dynamics is increasingly recognized to regulate myriad cell behaviors. We report a finding that intracellular pH dynamics also regulates adult stem cell lineage specification. We identify an intracellular pH gradient in mouse small intestinal crypts, lowest in crypt stem cells and increasing along the crypt column. Disrupting this gradient by inhibiting H+ efflux by Na+/H+ exchanger 1 abolishes crypt budding and blocks differentiation of Paneth cells, which are rescued with exogenous WNT. Using single-cell RNA sequencing and lineage tracing we demonstrate that intracellular pH dynamics acts downstream of ATOH1, with increased pH promoting differentiation toward the secretory lineage. Our findings indicate that an increase in pH is required for the lineage specification that contributes to crypt maintenance, establishing a role for intracellular pH dynamics in cell fate decisions within an adult stem cell lineage.
Endogenous learning for green hydrogen in a sector-coupled energy model for Europe
Authors: Zeyen Elisabeth , Victoria Marta , Brown Tom 
Published: June 2023

AbstractMany studies have shown that hydrogen could play a large role in the energy transition for hard-to-electrify sectors, but previous modelling has not included the necessary features to assess its role. They have either left out important sectors of hydrogen demand, ignored the temporal variability in the system or neglected the dynamics of learning effects. We address these limitations and consider learning-by-doing for the full green hydrogen production chain with different climate targets in a detailed European sector-coupled model. Here, we show that in the next 10 years a faster scale-up of electrolysis and renewable capacities than envisaged by the EU in the REPowerEU Plan can be cost-optimal to reach the strictest +1.5oC target. This reduces the costs for hydrogen production to 1.26 €/kg by 2050. Hydrogen production switches from grey to green hydrogen, omitting the option of blue hydrogen. If electrolysis costs are modelled without dynamic learning-by-doing, then the electrolysis scale-up is significantly delayed, while total system costs are overestimated by up to 13% and the levelised cost of hydrogen is overestimated by 67%.
Development of a Prevotella bivia PNA probe and a multiplex approach to detect three relevant species in bacterial vaginosis-associated biofilms
Authors: Sousa Lúcia G. V. , Almeida Carina , Muzny Christina A. , Cerca Nuno 
Published: June 2023

AbstractBacterial vaginosis (BV) is the most common vaginal infection worldwide. We developed a peptide nucleic acid (PNA) probe targeting Prevotella bivia, a common BV-associated bacteria, and optimized a multiplex approach for detection of Gardnerella spp., P. bivia and Fannyhessea vaginae. Our P. bivia PNA probe specifically detected the target species, and the optimized multiplex approach was able to detect the presence of the three species in multi-species BV biofilms.
Rare subtypes of triple negative breast cancer: Current understanding and future directions
Authors: Thomas Alexandra , Reis-Filho Jorge S. , Geyer Charles E. , Wen Hannah Y. 
Published: June 2023

AbstractRare subtypes of triple-negative breast cancers (TNBC) are a heterogenous group of tumors, comprising 5–10% of all TNBCs. Despite accounting for an absolute number of cases in aggregate approaching that of other less common, but well studied solid tumors, rare subtypes of triple-negative disease remain understudied. Low prevalence, diagnostic challenges and overlapping diagnoses have hindered consistent categorization of these breast cancers. Here we review epidemiology, histology and clinical and molecular characteristics of metaplastic, triple-negative lobular, apocrine, adenoid cystic, secretory and high-grade neuroendocrine TNBCs. Medullary pattern invasive ductal carcinoma no special type, which until recently was a considered a distinct subtype, is also discussed. With this background, we review how applying biological principals often applied to study TNBC no special type could improve our understanding of rare TNBCs. These could include the utilization of targeted molecular approaches or disease agnostic tools such as tumor mutational burden or germline mutation-directed treatments. Burgeoning data also suggest that pathologic response to neoadjuvant therapy and circulating tumor DNA have value in understanding rare subtypes of TNBC. Finally, we discuss a framework for advancing disease-specific knowledge in this space. While the conduct of randomized trials in rare TNBC subtypes has been challenging, re-envisioning trial design and technologic tools may offer new opportunities. These include embedding rare TNBC subtypes in umbrella studies of rare tumors, retrospective review of contemporary trials, prospective identification of patients with rare TNBC subtypes entering on clinical trials and querying big data for outcomes of patients with rare breast tumors.
An AI-driven microstructure optimization framework for elastic properties of titanium beyond cubic crystal systems
Authors: Mao Yuwei , Hasan Mahmudul , Paul Arindam , Gupta Vishu , Choudhary Kamal , Tavazza Francesca , Liao Wei-keng , Choudhary Alok , Acar Pinar , Agrawal Ankit 
Published: June 2023

AbstractMaterials design aims to identify the material features that provide optimal properties for various engineering applications, such as aerospace, automotive, and naval. One of the important but challenging problems for materials design is to discover multiple polycrystalline microstructures with optimal properties. This paper proposes an end-to-end artificial intelligence (AI)-driven microstructure optimization framework for elastic properties of materials. In this work, the microstructure is represented by the Orientation Distribution Function (ODF) that determines the volume densities of crystallographic orientations. The framework was evaluated on two crystal systems, cubic and hexagonal, for Titanium (Ti) in Joint Automated Repository for Various Integrated Simulations (JARVIS) database and is expected to be widely applicable for materials with multiple crystal systems. The proposed framework can discover multiple polycrystalline microstructures without compromising the optimal property values and saving significant computational time.
Shorter telomere length predicts poor antidepressant response and poorer cardiometabolic indices in major depression
Authors: Rampersaud Ryan , Wu Gwyneth W. Y. , Reus Victor I. , Lin Jue , Blackburn Elizabeth H. , Epel Elissa S. , Hough Christina M. , Mellon Synthia H. , Wolkowitz Owen M. 
Published: June 2023

AbstractTelomere length (TL) is a marker of biological aging, and shorter telomeres have been associated with several medical and psychiatric disorders, including cardiometabolic dysregulation and Major Depressive Disorder (MDD). In addition, studies have shown shorter TL to be associated with poorer response to certain psychotropic medications, and our previous work suggested shorter TL and higher telomerase activity (TA) predicts poorer response to Selective Serotonin Reuptake Inhibitor (SSRI) treatment. Using a new group of unmedicated medically healthy individuals with MDD (n = 48), we sought to replicate our prior findings demonstrating that peripheral blood mononuclear cell (PBMC) TL and TA predict response to SSRI treatment and to identify associations between TL and TA with biological stress mediators and cardiometabolic risk indices. Our results demonstrate that longer pre-treatment TL was associated with better response to SSRI treatment (β = .407p = .007). Additionally, we observed that TL had a negative relationship with allostatic load (β = − .320p = .017) and a cardiometabolic risk score (β = − .300p = .025). Our results suggest that PBMC TL reflects, in part, the cumulative effects of physiological stress and cardiovascular risk in MDD and may be a biomarker for predicting SSRI response.
Conduit design with expanding diameter for enhanced flow
Authors: Hashemi Javad , Peeples Hunter , Kuykendall Riley , Raju Seshadri , Kassab Ghassan S. 
Published: June 2023

AbstractConduits are commonly used for treating lesions in arteries and veins. The conventional stents are cylindrical in shape, which increases flow resistance with length. This study presents a design of stents and conduits where the conduit caliber expands gradually to reduce resistance while avoiding flow separation. Inflow was provided from a header tank at two different pressures (i.e., 10 and 25 mm Hg pressure) into a cylindrical or expanding conduit. The initial conduit calibers were 2-, 3-, 4-, and 5-mm and 160-, 310-, and 620-mm lengths in each case. The flow rates of expanding caliber conduits (at a rate of r4–6/cm where r is the initial conduit radius) were compared to traditional cylindrical conduits of constant radius. The expanded caliber yields a significantly increased flow of 16–55% for R4/L expansion, 9–44% for R5/L expansion, and 1–28% for R6/L expansion. Simulated flow models using computational fluid dynamics (CFD) were used to validate and expand the experimental findings. Flow separation was detected for certain simulations by flow pathlines and wall shear stress (WSS) calculations. The results showed that a caliber expansion rate of r6/cm is the optimal rate of expansion for most potential applications with minimum flow separation, lower resistance, and increased flow.
Reliability and validity of the Japanese version of the Ocular pain assessment survey (OPAS-J)
Authors: Yamanishi Ryutaro , Suzuki Natsume , Uchino Miki , Kawashima Motoko , Tsubota Kazuo , Negishi Kazuno 
Published: June 2023

AbstractThis study aimed to determine the reliability and validity of the Japanese version of the Ocular Pain Assessment Survey (OPAS-J) to measure ocular pain and quality of life. A multi-institutional cross-sectional study was conducted on participants with and without ocular pain. The Wong–Baker FACES® Pain Rating Scale served as the gold standard for measuring the intensity of ocular pain. Sixty-four participants who visited two clinics located in Japan between May 2019 and October 2019 were included in the study. The OPAS was translated and culturally adapted to Japanese. The internal consistency of the OPAS-J was assessed using Cronbach’s alpha coefficient. Twenty-four (37.5%) and 40 (62.5%) participants were classified as having ocular pain and no ocular pain, respectively. All dimensions of the OPAS-J had good reliability, with a Cronbach’s alpha coefficient of 0.870 for ocular pain intensity over the past 24 h and 0.874, 0.899, 0.874, 0.871, and 0.876 for ocular pain intensity over the past 2 weeks, non-ocular pain, interference with quality of life, aggravating factors, and associated factors, respectively. The OPAS-J is a reliable and responsive tool that can be used to quantify ocular pain intensity.
Phylogeny, ancestral ranges and reclassification of sand dollars
Authors: Lee Hsin , Lee Kwen-Shen , Hsu Chia-Hsin , Lee Chen-Wei , Li Ching-En , Wang Jia-Kang , Tseng Chien‑Chia , Chen Wei-Jen , Horng Ching-Chang , Ford Colby T. , Kroh Andreas , Bronstein Omri , Tanaka Hayate , Oji Tatsuo , Lin Jih-Pai , Janies Daniel 
Published: June 2023

AbstractClassification of the Class Echinoidea is under significant revision in light of emerging molecular phylogenetic evidence. In particular, the sister-group relationships within the superorder Luminacea (Echinoidea: Irregularia) have been considerably updated. However, the placement of many families remains largely unresolved due to a series of incongruent evidence obtained from morphological, paleontological, and genetic data for the majority of extant representatives. In this study, we investigated the phylogenetic relationships of 25 taxa, belonging to eleven luminacean families. We proposed three new superfamilies: Astriclypeoidea, Mellitoidea, and Taiwanasteroidea (including Dendrasteridae, Taiwanasteridae, Scutellidae, and Echinarachniidae), instead of the currently recognized superfamily Scutelloidea Gray, 1825. In light of the new data obtained from ten additional species, the historical biogeography reconstructed shows that the tropical western Pacific and eastern Indian Oceans are the cradle for early sand dollar diversification. Hothouse conditions during the late Cretaceous and early Paleogene were coupled with diversification events of major clades of sand dollars. We also demonstrate that Taiwan fauna can play a key role in terms of understanding the major Cenozoic migration and dispersal events in the evolutionary history of Luminacea.
Tau protein binds to the P53 E3 ubiquitin ligase MDM2
Authors: Sola Martina , Rendon-Angel Azucena , Rojo Martinez Viviana , Sgrignani Jacopo , Magrin Claudia , Piovesana Ester , Cavalli Andrea , Paganetti Paolo , Papin Stéphanie 
Published: June 2023

AbstractTau gene mutations cause a progressive dementia and neurotoxic Tau forms deposited in neurofibrillary tangles are hallmarks of neurodegenerative tauopathies. Loss of non-canonical Tau functions may contribute to disease. In fact, Tau depletion affects the cellular response to DNA damage and tauopathies exhibit the accumulation of DNA lesions. Moreover, Tau modulates P53 activity and cell fate. Considering that MDM2 is the main antagonist of P53, we investigated, using orthogonal assays, if Tau interacts with MDM2. We report the existence in cells and brain of a Tau-MDM2 complex that, in vitro, exhibits reduced P53 ubiquitination activity in a manner sensitive to a Tau mutation. The Tau-MDM2 interaction involves the microtubule-binding domain of Tau and the acidic domain of MDM2, reminiscent of the binding of Tau to negatively charged microtubules. Notably, MDM2 accumulates aberrantly in neurofibrillary tangles. Aging-associated insults may expose a novel loss-of-function of Tau in neurodegeneration and cancer.
Using macular velocity measurements to relate parameters of bone conduction to vestibular compound action potential responses
Authors: Pastras Christopher J. , Curthoys Ian S. , Rabbitt Richard D. , Brown Daniel J. 
Published: June 2023

AbstractTo examine mechanisms responsible for vestibular afferent sensitivity to transient bone conducted vibration, we performed simultaneous measurements of stimulus-evoked vestibular compound action potentials (vCAPs), utricular macula velocity, and vestibular microphonics (VMs) in anaesthetized guinea pigs. Results provide new insights into the kinematic variables of transient motion responsible for triggering mammalian vCAPs, revealing synchronized vestibular afferent responses are not universally sensitive to linear jerk as previously thought. For short duration stimuli (< 1 ms), the vCAP increases magnitude in close proportion to macular velocity and temporal bone (linear) acceleration, rather than other kinematic elements. For longer duration stimuli, the vCAP magnitude switches from temporal bone acceleration sensitive to linear jerk sensitive while maintaining macular velocity sensitivity. Frequency tuning curves evoked by tone-burst stimuli show vCAPs increase in proportion to onset macular velocity, while VMs increase in proportion to macular displacement across the entire frequency bandwidth tested between 0.1 and 2 kHz. The subset of vestibular afferent neurons responsible for synchronized firing and vCAPs have been shown previously to make calyceal synaptic contacts with type I hair cells in the striolar region of the epithelium and have irregularly spaced inter-spike intervals at rest. Present results provide new insight into mechanical and neural mechanisms underlying synchronized action potentials in these sensitive afferents, with clinical relevance for understanding the activation and tuning of neurons responsible for driving rapid compensatory reflex responses.
Effects of different training schedules based on distance and accelerometry measures: A full-season case study in professional soccer players
Authors: Nobari Hadi , Alijanpour Nader , Talvari Amirhossein , Oliveira Rafael 
Published: June 2023

AbstractThis study aimed to evaluate external load based on distance and accelerometry measures in six different microcycle schedules with congested (CW2, CW3, and CW4) and regular weeks (RW4, RW5, and RW6) in professional soccer players. Twelve Iranian First League players (age: 28.2 ± 3.8 years; body mass: 77.92 ± 4.72 kg, and height: 183.2 ± 0.06 cm) participated in this study. A GPSPORTS system was used to collect training/match durations, total distance, distance covered at different speeds, number of accelerations, delecelerations and body load over 29 weeks, 40 matches, and 121 training sessions. Data were analysed by weekly mean and accumulated weekly values. The results showed a tendency for higher values in CWs than RWs. Regarding mean total distance, RW4 and RW5 presented lower values with very large effect size than CW2 (all, p < 0.05). The mean distance covered between 16 and 23 km/h showed that all RWs presented significantly lower values than all CWs (p < 0.05 with considerable effect size). In addition, mean acceleration values at < 2 m/s2 showed significantly lower values than all CWs (p < 0.05 with a very large effect size). Therefore, it is recommended to coaches pay special attention to players who participate in matches to reduce fatigue and improve the performance of professional soccer players. Ensuring recovery mechanisms are in place to minimize the external load is recommended. Coaches arrange practice sessions for non-starters who do not experience similar external loads compared to starters to prepare them for potential competition.