bluuebunny/crossref_metadata_2025_split huggingface dataset:
The promoting effects of GPR176 expression on proliferation, chemoresistance, lipogenesis and invasion of oesophageal cancer
Authors: Yun Wen-jing , Li Jun , Yin Nan-chang , Zhang Cong-yu , Cui Zheng-guo , Zhang Li , Zheng Hua-chuan 
Published: August 2023

AbstractPurposeAs a member of the G-protein-coupled receptor 1 family, the G-protein-coupled receptor 176 (GPR176) gene encodes a glycosylated protein made up of 515 amino acids. The current study was performed to evaluate the impact of GPR176 on the clinicopathology and prognosis of oesophageal cancer, as well as uncover its molecular mechanisms.MethodsBioinformatics and clinical tissue samples were used to detect the expression and clinicopathological significance of GPR176 in oesophageal cancer. The expression, proliferation, migration and invasion, apoptosis and lipid droplet formation of GPR176 gene in oesophageal cancer were performed as phenotypic readouts.ResultsHere, RT-PCR and bioinformatic analyses revealed that GPR176 mRNA expression was significantly higher in oesophageal cancer than in normal mucosa (p < 0.05). GPR176 mRNA expression was associated with low weight and BMI, low T stage, low N and clinicopathological stage, low histological grade and favourable clinical outcome of oesophageal cancer (p < 0.05). The differential genes of GPR176 mRNA were involved in protein digestion and absorption, extracellular matrix constituent, endoplasmic reticulum lumen, among others (p < 0.05). GPR176-related genes were classified as being involved in oxidoreductase activity, actin and myosin complexes, lipid localisation and transport, among others (p < 0.05). GPR176 knockdown suppressed proliferation, anti-apoptotic and anti-pyroptotic properties, migration, invasion, chemoresistance and lipid droplet formation in oesophageal cancer cells (p < 0.05), while ACC1 and ACLY overexpression reversed the inhibitory effects of GPR176 silencing on lipid droplet formation and chemoresistance.ConclusionThese findings indicated that upregulated expression of GPR176 might be involved in oesophageal carcinogenesis and subsequent progression, aggressiveness, and induced chemoresistance by ACC1- and ACLY-mediated lipogenesis and lipid droplet assembly.
In vitro antineoplastic effects of MK0752 in HPV-positive head and neck squamous cell carcinoma
Authors: Varatanovic Sara , Stoiber Stefan , Haas Markus , Lein Alexander , Kadletz-Wanke Lorenz , Brkic Faris F. 
Published: August 2023

Abstract
Purpose
Gamma-secretase inhibitor MK0752 has shown a high therapeutic potential in different solid malignant tumors. Up to now, its antineoplastic effects were not investigated in head and neck squamous cell carcinoma (HNSCC) and particularly in human-papillomavirus (HPV)-positive tumors.

Methods
We conducted cytotoxic, migration, and clonogenic assays in two HPV-negative HNSCC cell lines (Cal27 and FaDu) and one HPV-positive cell line (SCC154). Furthermore, in order to assess the pro-apoptotic effects of MK0752, a Caspase 3/7 Glo assay was performed.

Results
Our experiments revealed antineoplastic effects of MK0752 in all three cell lines. Strong cytotoxic and antimigratory potential was shown in all cell lines, with strongest effects observed in the HPV-positive cell line. Meanwhile, anticlonogenic effects were only shown in Cal27 and SCC154. Most importantly, MK0752 induced apoptosis solely in HPV-positive SCC154.

Conclusions
Our novel findings indicate a therapeutic potential of MK0752 in HPV-positive HNSCC. Indeed, further investigation is needed for validation of our results and for the assessment of the mechanistic background.
The combined signatures of G protein-coupled receptor family and immune landscape provide a prognostic and therapeutic biomarker in endometrial carcinoma
Authors: Chen Shengyue , Luo Xukai , Yang Baicai , Zhuang Jingming , Guo Jinshuai , Zhu Yingjie , Mo Jiahang 
Published: August 2023

AbstractG protein-coupled receptors (GPRs) are one of the largest surface receptor superfamilies, and many of them play essential roles in biological processes, including immune responses. In this study, we aim to construct a GPR- and tumor immune environment (TME-i)-associated risk signature to predict the prognosis of patients with endometrial carcinoma (EC). The GPR score was generated by applying univariate Cox regression and the Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression in succession. This involved identifying the differentially expressed genes (DEGs) in the Cancer Genome Atlas-Uterine Corpus Endometrioid Carcinoma (TCGA-UCEC) cohort. Simultaneously, the CIBERSORT algorithm was applied to identify the protective immune cells for TME score construction. Subsequently, we combined the GPR and TME scores to establish a GPR-TME classifier for conducting clinical prognosis assessments. Various functional annotation algorithms were used to conduct biological process analysis distinguished by GPR-TME subgroups. Furthermore, weighted correlation network analysis (WGCNA) was applied to depict the tumor somatic mutations landscapes. Finally, we compared the immune-related molecules between GPR-TME subgroups and resorted to the Tumor Immune Dysfunction and Exclusion (TIDE) for immunotherapy response prediction. The mRNA and protein expression of GPR-related gene P2RY14 were, respectively, validated by RT-PCR in clinical samples and HPA database. To conclude, our GPR-TME classifier may aid in predicting the EC patients’ prognosis and immunotherapy responses.
The use of hybrid operating rooms in neurosurgery, advantages, disadvantages, and future perspectives: a systematic review
Authors: Gharios Maria , El-Hajj Victor Gabriel , Frisk Henrik , Ohlsson Marcus , Omar Artur , Edström Erik , Elmi-Terander Adrian 
Published: August 2023

Abstract
Background
Hybrid operating rooms (hybrid-ORs) combine the functionalities of a conventional surgical theater with the advanced imaging technologies of a radiological suite. Hybrid-ORs are usually equipped with CBCT devices providing both 2D and 3D imaging capability that can be used for both interventional radiology and image guided surgical applications. Across all fields of surgery, the use of hybrid-ORs is gaining in traction, and neurosurgery is no exception. We hence aimed to comprehensively review the use of hybrid-ORs, the associated advantages, and disadvantages specific to the field of neurosurgery.

Materials and methods
Electronic databases were searched for all studies on hybrid-ORs from inception to May 2022. Findings of matching studies were pooled to strengthen the current body of evidence.

Results
Seventy-four studies were included in this review. Hybrid-ORs were mainly used in endovascular surgery (n = 41) and spine surgery (n = 33). Navigation systems were the most common additional technology employed along with the CBCT systems in the hybrid-ORs. Reported advantages of hybrid-ORs included immediate assessment of outcomes, reduced surgical revision rate, and the ability to perform combined open and endovascular procedures, among others. Concerns about increased radiation exposure and procedural time were some of the limitations mentioned.

Conclusion
In the field of neurosurgery, the use of hybrid-ORs for different applications is increasing. Hybrid-ORs provide preprocedure, intraprocedure, and end-of-procedure imaging capabilities, thereby increasing surgical precision, and reducing the need for postoperative imaging and correction surgeries. Despite these advantages, radiation exposure to patient and staff is an important concern.
Assessment of Membrane and Electrochemical Technologies for the Treatment of a Selenium-Bearing Mine water: Technology Performance and Effect on Toxicity and Bioaccumulation
Authors: Baghbanzadeh Mohammadali , Dillon Sylvain , Volchek Konstantin , Rickwood Carrie , Goulet Richard R. 
Published: August 2023

AbstractNanofiltration (NF), reverse osmosis (RO), electrodialysis (ED), and electrocoagulation (EC), were all tested at the bench scale for removing selenium (Se) from mine water. All of these technologies reduced the concentration of total Se from 216 µg/L (i.e. 120.1 µg/L of selenate; 59.1 µg/L of selenite, and 0.6 µg/L methyl-selenic acid) in the raw mine water to about 2 µg/L or less in the treated water, equivalent to more than 99% removal. Electrodialysis was found to be the most effective, removing more than 99.5% of the Se. The untreated mine water was toxic to algae. In contrast, RO and NF reduced the toxicity of the mine water, allowing algae to grow between 15,000 to 25,000 cells/mL, while ED and EC did not allow algal growth, likely due to complete removal of essential minerals (ED) or the presence of other contaminants (EC), such as copper. The Se speciation did not change as a result of membrane filtration; however, selenite in the effluent was almost fully transformed to selenate in the brines from the ED and EC treatment processes. The effluent treated by NF and EC generated seleno-cyanate at 0.37 and 1.01 µg/L, respectively. Further testing is recommended at the pilot-scale with the same mine water as well as different mine water.
A review of contrast-enhanced harmonic endoscopic ultrasonography for pancreatic solid tumors
Authors: Otsuka Yasuo , Kamata Ken 
Published: August 2023

AbstractEndoscopic ultrasonography (EUS) is superior to other imaging modalities in the detection of pancreatic masses, although differentiating the types of pancreatic masses detected on EUS remains challenging. However, the value of contrast-enhanced harmonic EUS (CH-EUS) using ultrasound contrast agents for this differentiation has been reported. CH-EUS plays a pivotal role in analysis of small lesions that can only be detected with EUS. Recently, CH-EUS was used for staging and/or determining the resectability of pancreatic cancer in several clinical trials. In addition, it is used to estimate the response of pancreatic cancer to chemotherapy and to determine the prognosis in cases of pancreatic cancer and pancreatic neuroendocrine neoplasms. It is also postulated that CH-EUS improves the diagnostic performance of endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNAB) through complementary diagnoses using CH-EUS and EUS-FNAB, or CH-EUS-guided EUS-FNAB. Thus, CH-EUS has been employed for various qualitative diagnoses, including differentiation of pancreatic masses. Second-generation contrast agents such as Sonazoid are used clinically for ultrasound diagnostic imaging of liver and breast disease. The positioning of CH-EUS with Sonazoid as a test for the diagnosis of solid pancreatic tumors is an issue for further studies.
Assessing resilience at different scales: from single assets to complex systems
Authors: Fuggini Clemente , Solari Celina , De Stefano Rita , Bolletta Fabio , De Maio Florencia Victoria 
Published: August 2023

AbstractNowadays, critical infrastructure and systems are getting more and more interconnected, while facing increasing and more intensive hazards: from man-made to natural ones, including those exacerbated by effects of the climate change. The demand for their robustness and resiliency against all these threats is finding ground to organizations or states’ ambitions, implementations, and policies. Moreover, their distributed network spanning from local areas to cities, from regions to cross-country extension, make them a target for malicious actions aimed to damage or even disrupt their critical supplied and therefore the availability of the service they deliver. The paper focuses on a review from an engineering perspective of past efforts (namely those related to the H2020 Secure Gas project) and provides evidence of application cases where the network/system dimension of the critical infrastructure is a key point to be taken into account and to be safeguarded. Finally, an outlook on future perspectives and potentials in the application of resilience at local, urban and territorial/national level is described, with incoming and emerging threats at local and global level.
“Every Day has Enough of Its Own Torment”—A Narrative Study of Life’s Greatest Challenge Among Men in Late Adulthood
Authors: Wängqvist Maria , Eriksson Py Liv 
Published: August 2023

AbstractThe purpose of this study was to investigate stories of life’s greatest challenge among men in late adulthood from a narrative and developmental perspective. The investigations focused on narrative processes and the content of challenge narratives in relation to satisfaction with life and generativity. Narrative processes were analyzed using existing frameworks (Eriksson et al. Identity 20:157–169, 2020). The study showed negative, neutral/vague, redemptive, and a combination of positive and negative emotional sequences, among which negative sequencing was the most common. An additional narrative theme, metareflections of challenges as part of life, involved the conclusion that hardship is simply part of what one may expect from life. Analyses of types of challenges revealed six categories and a secondary coding the adversity of the challenges. In contrast to expectations, redemptive sequencing was not associated with either higher satisfaction with life or generativity. The few differences that emerged in the subsequent analyses showed that participants whose challenges were coded as not expected and potentially disruptive had significantly lower satisfaction with life and lower mean age when the challenge occurred. In conclusion, the study demonstrated the saliency of the challenges’ timing and adversity. Negative framing was common in the challenge narratives of the men in late adulthood, without being negatively associated with satisfaction with life or generativity. The role of negative emotional sequencing in this study adds new perspectives to the emphasis on redemption and positive emotionality for well-being and generativity in later adulthood.
Qβ, Qc, Qi, Qs of the Gargano Promontory (Southern Italy)
Authors: Lucente Salvatore , Ninivaggi Teresa , de Lorenzo Salvatore , Del Pezzo Edoardo , Filippucci Marilena , Prosser Giacomo , Tallarico Andrea 
Published: August 2023

AbstractWe have provided the first estimate of scattering and intrinsic attenuation for the Gargano Promontory (Southern Italy) analyzing 190 local earthquakes with ML ranging from 1.0 to 2.8. To separate the intrinsic $${Q}_{i}$$

Q
i

and scattering $${Q}_{s}$$

Q
s

quality factors with the Wennerberg approach (1993), we have measured the direct S waves and coda quality factors ($${Q}_{\beta }$$

Q
β

, $${Q}_{c}$$

Q
c

) in the same volume of crust. $${Q}_{\beta }$$

Q
β

parameter is derived with the coda normalization method (Aki 1980) and $${Q}_{c}$$

Q
c

factor is derived with the coda envelope decay method (Sato 1977). We selected the coda envelope by performing an automatic picking procedure from $${T}_{\mathrm{start}}=1.5{T}_{S}$$

T
start

=
1.5

T
S

up to 30 s after origin time (lapse time $${T}_{L}$$

T
L

). All the obtained quality factors clearly increase with frequency. The $${Q}_{c}$$

Q
c

values correspond to those recently obtained for the area. The estimated $${Q}_{i}$$

Q
i

are comparable to the $${Q}_{c}$$

Q
c

at all frequencies and range between 100 and 1000. The $${Q}_{s}$$

Q
s

parameter shows higher values than $${Q}_{i}$$

Q
i

, except for 8 Hz, where the two estimates are closer. This implies a predominance of intrinsic attenuation over the scattering attenuation. Furthermore, the similarity between $${Q}_{i}$$

Q
i

and $${Q}_{c}$$

Q
c

allows us to interpret the high $${Q}_{c}$$

Q
c

anomaly previously found in the northern Gargano Promontory up to a depth of 24 km, as a volume of crust characterized by very low seismic dumping produced by conversion of seismic energy into heat. Moreover, most of the earthquake foci fall in high $${Q}_{i}$$

Q
i

areas, indicating lower level of anelastic dumping and a brittle behavior of rocks.
Genome-wide identification and expression analysis of the trihelix transcription factor family in sesame (Sesamum indicum L.) under abiotic stress
Authors: Zhao Yunyan , Liang Junchao , Wang Zhiqi , Yan Tingxian , Yan Xiaowen , Wei Wenliang , Le Meiwang , Sun Jian 
Published: August 2023

Abstract
Background
The plant trihelix gene family is among the earliest discovered transcription factor families, and it is vital in modulating light, plant growth, and stress responses.

Methods
The identification and characterization of trihelix family members in the sesame genome were analyzed by bioinformatics methods, and the expression patterns of sesame trihelix genes were assessed by quantitative real-time PCR.

Results
There were 34 trihelix genes discovered in the genome of sesame, which were irregularly distributed among 10 linkage groups. Also, the genome contained 5 duplicate gene pairs. The 34 trihelix genes were divided into six sub-families through a phylogenetic study. A tissue-specific expression revealed that SiTH genes exhibited spatial expression patterns distinct from other trihelix genes in the same subfamily. The cis-element showed that the SiTHs gene promoter contained various elements associated with responses to hormones and multiple abiotic stresses. Additionally, the expression patterns of 8 SiTH genes in leaves under abiotic stresses demonstrated that all selected genes were significantly upregulated or downregulated at least once in the stress period. Furthermore, the SiTH4 gene was significantly induced in response to drought and salt stress, showing that SiTH genes may be engaged in the stress response mechanisms of sesame.

Conclusion
These findings establish a foundation for further investigation of the trihelix gene-mediated response to abiotic stress in sesame.
Immuno markers in newly diagnosed glioblastoma patients underwent Stupp protocol after neurosurgery: a retrospective series
Authors: Gurrieri Lorena , Mercatali Laura , Ibrahim Toni , Fausti Valentina , Dall’Agata Monia , Riva Nada , Ranallo Nicoletta , Pasini Giuseppe , Tazzari Marcella , Foca Flavia , Bartolini Daniela , Riccioni Luca , Cavatorta Chiara , Morigi Federico Paolo , Bulgarelli Jenny , Cocchi Claudia , Ghini Virginia , Tosatto Luigino , Martinelli Giovanni , Pession Andrea , Ridolfi Laura 
Published: August 2023

Abstract
Purpose
The aims of our retrospective study investigated the role of immune system in glioblastoma (GBM), which is the most aggressive primary brain tumor in adults characterized by a poor prognosis. The recurrence rate remains high, probably due to “immune-desert” tumor microenvironment (TME) making GBM hidden from the anti-tumoral immune clearance. Considering this, we aimed to create a panel of prognostic markers from blood and tumor tissue correlating with overall survival (OS) and progression-free survival (PFS).

Methods
Firstly, we analyzed the inflammatory markers NLR and PLR as the ratio of the absolute neutrophil count and absolute platelet count by the absolute lymphocyte count respectively, collected at different time points in the peripheral blood of 95 patients. Furthermore, in 31 patients of the same cohort, we analyzed the formalin-fixed paraffin embedded samples to further compare the impact of circulating and inflammatory markers within the TME.

Results
Patients aged < 60 years and with methylated MGMT showed better OS. While, pre-chemotherapy Systemic Inflammatory Index (SII) < 480 was related to a better OS and PFS, we observed that only CD68+macrophage and CD66b+neutrophils expressed in vascular/perivascular area (V) showed a statistically significant prognostic role in median OS and PFS.

Conclusions
Thus, we underscored a role of SII as predictive value of response to STUPP protocol. Regarding the TME-related markers, we suggested to take into consideration for future studies with new immunotherapy combinations, each component relating to expression of immune infiltrating subsets.
Renormalized Von Neumann entropy with application to entanglement in genuine infinite dimensional systems
Authors: Gielerak Roman 
Published: August 2023

AbstractA renormalized version of the von Neumann quantum entropy (which is finite and continuous in general, infinite dimensional case) which obeys several of the natural physical demands (as expected for a “good” measure of entanglement in the case of general quantum states describing bipartite and infinite-dimensional systems) is proposed. The renormalized quantum entropy is defined by the explicit use of the Fredholm determinants theory. To prove the main results on continuity and finiteness of the introduced renormalization, the fundamental Grothendick approach, which is based on the infinite dimensional Grassmann algebra theory, is applied. Several features of majorization theory are preserved under the introduced renormalization as it is proved in this paper. This fact allows to extend most of the known (mainly, in the context of two-partite, finite-dimensional quantum systems) results of the LOCC comparison theory to the case of genuine infinite-dimensional, two-partite quantum systems.
Conversion of waste biomass to designed and tailored activated chars with valuable properties for adsorption and electrochemical applications
Authors: Januszewicz Katarzyna , Kazimierski Paweł , Cymann-Sachajdak Anita , Hercel Paulina , Barczak Beata , Wilamowska-Zawłocka Monika , Kardaś Dariusz , Łuczak Justyna 
Published: August 2023

AbstractWaste biomass, a renewable energy source, is inexpensive material that has great potential in sorption and electrochemical application. The selected waste materials (corncobs, coconut shells, walnuts, and pistachio husks) allow to close the production cycle and enable material recycling, which are important aspects in the hierarchy of waste management. The proposed methodology for production and activation of biochars can be used industrially due to highly porous structure, developed surface area, and sorption ability of the obtained activated carbons (AC). A significant increase (from 4 up to more than 10 times) in specific surface area (SSA) is observed for all samples after the CO2 activation process (0.5 h at 800 °C) up to 725 m2 g−1 for corncobs, 534.9 m2 g−1 for pistachio husks, 523 m2 g−1 for coconut shells, and 393 m2 g−1 for walnut husks. The highest value of SSA is achieved for the AC derived from corncobs. This material is evaluated for use as an adsorbent, revealing 99% removal of Rhodamine B (dye/AC ratio of 0.0017) and 69% removal of chromium (dye/AC ratio of 0.0028). Based on the adsorption kinetics analysis, it is demonstrated that the Cr(VI) undergoes physical adsorption, while RhB undergoes chemisorption. In addition, corncob-derived AC exhibits superior electrochemical performance in 6 M KOH compared to the nonactivated biochar. A specific capacitance of 70 F g−1 at 5 A g−1 is achieved, along with outstanding rate capability (45 F g−1 at 50 A g−1) and cycling stability (94% at 10 A g−1 after 10,000 cycles). In contrast, the nonactivated sample shows only 34 F g−1 at 5 A g−1 and 13 F g−1 at 50 A g−1, with a stability of 91.4%.
Playbacktheater als Instrument zum Konfliktmanagement
Authors: Schubbe Oliver 
Published: August 2023

ZusammenfassungPlaybacktheater ist eine Form des Improvisationstheaters, die sich aus Psychodrama, Stegreifspiel und mündlichen Erzähltraditionen entwickelt hat. Dieser Artikel der Zeitschrift für Psychodrama und Soziometrie beschreibt den Einsatz im Rahmen sozialen Konfliktmanagements. Es wird gezeigt, wie Rahmen, Regeln und Rituale des Playbacktheaters es Konfliktparteien erleichtern können, sich in einem moderierten Prozess zu begegnen, in dem Momente der Deeskalation, positive soziale Resonanz und verbindende Ideen entstehen können. Darüber hinaus wird die Möglichkeit diskutiert, traumatische Erfahrungen in einem solchen Rahmen aufzuarbeiten und zur Weiterentwicklung des kollektiven Selbstverständnisses von sozialen Gruppen beizutragen. Anhand von Beispielen aus Aufführungen wird die Befreiung von belastenden Geschichten gezeigt, und wie im Laufe der Aufführung ein thematischer „Roter Faden“ entsteht.