bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of superhydrophobic graphene-based coating on steel substrate and its ultraviolet durability and corrosion resistance properties
Authors: Mohamed M. E. , Mekhaiel P. S. , Mahgoub F. M. 
Published: January 2023

AbstractFor the first time, a facile and environmentally friendly approach for producing high-quality graphene from the biomass of banana leaves is described in this paper. Two rough coats of Ni-graphene, Ni@G, and Ni-graphene doped with chromium, Ni@Cr-G, were created on steel substrates by electrostatic deposition. These coatings were then submerged in an ethanolic solution of myristic acid, MA, to produce a superhydrophobic, SHP, surface. The Raman spectra demonstrated that the generated graphene was of high quality. Fourier transform infrared spectroscopy findings confirm the modification of the Ni@G coating by MA, Ni@G@MA, and the modification of the Ni@Cr-G composite with MA, Ni@Cr-G@MA. The results of the scanning electron microscope revealed that the created SHP coatings have nanoscale features. The wettability results showed that the water contact angle values for Ni@G@MA and Ni@Cr-G@MA coatings are 158° and 168°, while the water sliding angle values for both coatings are 4.0oand 1.0°, respectively. The atomic force microscopy results show that both Ni@G and Ni@Cr-G coatings increase the roughness of the steel. The chemical and mechanical stability of the Ni@Cr-G@MA coating was higher than those of the Ni@G@MA coating. The coated steel by Ni@Cr-G@MA exhibits UV stability up to 110 h, while the SHP-coated steel by Ni@G@MA exhibits UV stability for 60 h. The potentiodynamic polarization results show that the value of the corrosion current density for bare steel is 13 times that of steel coated with Ni@G@MA, and 21 times that of coated steel with Ni@Cr-G@MA. The electrochemical impedance spectroscopy, EIS, results show that the charge transfer resistance for steel coated with Ni@G@MA is 38 times that of bare steel, while steel coated with Ni@Cr-G@MA is 57 times that of bare steel. Potentiodynamic polarization and EIS results show that the SHP Ni@Cr-G@MA film exhibits higher corrosion resistance than Ni@G@MA film.
Nano-liter perfusion microfluidic device made entirely by two-photon polymerization for dynamic cell culture with easy cell recovery
Authors: McLennan Hanna J. , Blanch Adam J. , Wallace Samuel J. , Ritter Lesley J. , Heinrich Shauna L. , Gardner David K. , Dunning Kylie R. , Gauvin Marty J. , Love Allison K. , Thompson Jeremy G. 
Published: January 2023

AbstractPolydimethylsiloxane (PDMS) has been the material of choice for microfluidic applications in cell biology for many years, with recent advances encompassing nano-scaffolds and surface modifications to enhance cell-surface interactions at nano-scale. However, PDMS has not previously been amenable to applications which require complex geometries in three dimensions for cell culture device fabrication in the absence of additional components. Further, PDMS microfluidic devices have limited capacity for cell retrieval following culture without severely compromising cell health. This study presents a designed and entirely 3D-printed microfluidic chip (8.8 mm × 8.2 mm × 3.6 mm) using two-photon polymerization (2PP). The ‘nest’ chip is composed of ten channels that deliver sub-microliter volume flowrates (to ~ 600 nL/min per channel) to 10 individual retrievable cell sample ‘cradles’ that interlock with the nest to create the microfluidic device. Computational fluid dynamics modelling predicted medium flow in the device, which was accurately validated by real-time microbead tracking. Functional capability of the device was assessed, and demonstrated the capability to deliver culture medium, dyes, and biological molecules to support cell growth, staining and cell phenotype changes, respectively. Therefore, 2PP 3D-printing provides the precision needed for nanoliter fluidic devices constructed from multiple interlocking parts for cell culture application.
Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia
Authors: Filippenkov Ivan B. , Remizova Julia A. , Denisova Alina E. , Stavchansky Vasily V. , Golovina Ksenia D. , Gubsky Leonid V. , Limborska Svetlana A. , Dergunova Lyudmila V. 
Published: January 2023

AbstractIschemic stroke is one of the most severe polygenic brain diseases. Here, we performed further functional genetic analysis of the processes occurring in the contralateral hemisphere (CH) after ischemia–reperfusion injury in rat brain. Comparison of RNA sequencing data for subcortical samples from the ipsilateral hemisphere (IH) and CH after 90 min of transient middle cerebral artery occlusion (tMCAO) and corresponding sham-operated (SO) controls showed four groups of genes that were associated with ischemic processes in rat brain at 24 h after tMCAO. Among them, 2672 genes were differentially expressed genes (DEGs) for IH but non-DEGs for CH, 34 genes were DEGs for CH but non-DEGs for IH, and 114 genes had codirected changes in expression in both hemispheres. The remaining 16 genes exhibited opposite changes at the mRNA level in the two brain hemispheres after tMCAO. These findings suggest that the ischemic process caused by a focal ischemia induces complex bilateral reactions at the transcriptome level in the rat brain. We believe that specific genome responses in the CH and IH may provide a useful model for the study of the potential for brain repair after stroke.
Single-nuclei transcriptomics enable detection of somatic variants in patient brain tissue
Authors: Townsend Sydney E. , Westfall Jesse J. , Navarro Jason B. , Koboldt Daniel C. , Mardis Elaine R. , Miller Katherine E. , Bedrosian Tracy A. 
Published: January 2023

AbstractSomatic variants are a major cause of human disease, including neurological disorders like focal epilepsies, but can be challenging to study due to their mosaicism in bulk tissue biopsies. Coupling single-cell genotype and transcriptomic data has potential to provide insight into the role somatic variants play in disease etiology, such as by determining what cell types are affected or how the mutations affect gene expression. Here, we asked whether commonly used single-nucleus 3’- or 5’-RNA-sequencing assays can be used to derive single-nucleus genotype data for a priori known variants that are located near to either end of a transcript. To that end, we compared performance of commercially available single-nuclei 3’- and 5’- gene expression kits using resected brain samples from three pediatric patients with focal epilepsy. We quantified the ability to detect genetic variants in single-nucleus datasets depending on distance from the transcript end. Finally, we demonstrated the ability to identify affected cell types in a patient with a RHEB somatic variant causing an epilepsy-associated cortical malformation. Our results demonstrate that single-nuclei 3’ or 5’-RNA-sequencing data can be used to identify known somatic variants in single-nuclei when they are expressed within proximity to a transcript end.
Spatial filtering magnetic metasurface for misalignment robustness enhancement in wireless power transfer applications
Authors: Lazzoni Valeria , Brizi Danilo , Monorchio Agostino 
Published: January 2023

AbstractIn this paper, we present the design of spatial filtering magnetic metasurfaces to overcome the efficiency decay arising in misaligned resonant inductive Wireless Power Transfer systems. At first, we describe the analytical framework for the control of currents flowing on a finite-size metasurface, avoiding classical truncation effects on the periphery and opportunely manipulating, at the same time, the spatial magnetic field distribution produced by the closely placed RF driving coil. In order to validate the theoretical approach, we conceive a numerical test case consisting of a WPT system operating at 12 MHz. By performing accurate full-wave simulations, we prove that inducing a uniform current in the metasurface results in a more robust WPT system in terms of misalignment with respect to conventional configurations, also including standard metasurfaces. Therefore, while the use of metasurfaces in WPT systems has been already demonstrated to be beneficial in terms of efficiency enhancement, we confirmed that a proper control of the metasurfaces field filtering response can be advantageous also for the misalignment issue. Notably, the free space wavelength at the operating frequency (12 MHz) is 25 m, whereas the proposed metasurface dimensions are only 0.0024λ × 0.0024λ. Despite the extremely reduced dimensions, the spatial magnetic field distribution produced by the closely placed RF driving coil can be nevertheless opportunely manipulated. Finally, experimental measurements conducted on fabricated prototypes validated the numerical results, demonstrating the effectiveness of the proposed approach. These achievements can be particularly helpful in WPT applications where the position of driving and receiving coils frequently changes, as in consumer devices and biomedical implants.
Salmonella enterica serovars in absence of ttrA and pduA genes enhance the cell immune response during chick infections
Authors: Cabrera Julia M. , Saraiva Mauro M. S. , Rodrigues Alves Lucas B. , Monte Daniel F. M. , Vasconcelos Rosemeri O. , Freitas Neto Oliveiro C. , Berchieri Junior Angelo 
Published: January 2023

AbstractSalmonella spp. is one of the major foodborne pathogens responsible for causing economic losses to the poultry industry and bringing consequences for public health as well. Both the pathogen survival ability in the intestinal environment during inflammation as well as their relationship with the host immune system, play a key role during infections in poultry. The objective of this study was to quantify the presence of the macrophages and CD4+/CD8+ cells populations using the immunohistochemistry technique, in commercial lineages of chickens experimentally infected by wild-type and mutant strains of Salmonella Enteritidis and Salmonella Typhimurium lacking ttrA and pduA genes. Salmonella Enteritidis ∆ttrA∆pduA triggered a higher percentage of the stained area than the wild-type, with exception of light laying hens. Salmonella Typhimurium wild-type strain and Salmonella Typhimurium ∆ttrA∆pduA infections lead to a similar pattern in which, at 1 and 14 dpi, the caecal tonsils and ileum of birds showed a more expressive stained area compared to 3 and 7 dpi. In all lineages studied, prominent infiltration of macrophages in comparison with CD4+ and CD8+ cells was observed. Overall, animals infected by the mutant strain displayed a positively stained area higher than the wild-type. Deletions in both ttrA and pduA genes resulted in a more intense infiltration of macrophages and CD4+ and CD8+ cells in the host birds, suggesting no pathogen attenuation, even in different strains of Salmonella.
Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae)
Authors: Hejníčková Martina , Dalíková Martina , Zrzavá Magda , Marec František , Lorite Pedro , Montiel Eugenia E. 
Published: January 2023

AbstractThe W chromosome of Lepidoptera is typically gene-poor, repeat-rich and composed of heterochromatin. Pioneering studies investigating this chromosome reported an abundance of mobile elements. However, the actual composition of the W chromosome varies greatly between species, as repeatedly demonstrated by comparative genomic hybridization (CGH) or fluorescence in situ hybridization (FISH). Here we present an analysis of repeats on the W chromosome in the willow beauty, Peribatodesrhomboidaria (Geometridae), a species in which CGH predicted an abundance of W-enriched or W-specific sequences. Indeed, comparative analysis of male and female genomes using RepeatExplorer identified ten putative W chromosome-enriched repeats, most of which are LTR or LINE mobile elements. We analysed the two most abundant: PRW LINE-like and PRW Bel-Pao. The results of FISH mapping and bioinformatic analysis confirmed their enrichment on the W chromosome, supporting the hypothesis that mobile elements are the driving force of W chromosome differentiation in Lepidoptera. As the W chromosome is highly underrepresented in chromosome-level genome assemblies of Lepidoptera, this recently introduced approach, combining bioinformatic comparative genome analysis with molecular cytogenetics, provides an elegant tool for studying this elusive and rapidly evolving part of the genome.
Diversity of binary toxin positive Clostridioides difficile in Korea
Authors: Kim Jieun , Kim Bongyoung , Pai Hyunjoo 
Published: January 2023

AbstractThe objective of this study is to determine the trend and diversity of binary toxin-positive Clostridioides difficile over 10 years in Korea. Binary toxin-positive strains were selected from a tertiary hospital in Korea in 2009–2018. The multi-locus sequence typing and antibiotic susceptibility test were performed. Among the 3278 isolates in 2009–2018, 58 possessed binary toxin genes (1.7%). The proportion of CDT- positive isolates was 0.51–4.82% in 2009–2018, which increased over the 10-year period (P = 0.023). Thirteen sequence types (STs) were identified; ST5 (14 [24%]), ST11 (11 [19%]), ST221 (10 [17%]), ST201 (7 [12%]) and ST1 (5 [9%]) were popular. All 58 isolates were susceptible to vancomycin and piperacillin/tazobactam, and clindamycin and moxifloxacin were active in 69.0% and 62% of isolates, respectively. ST1 strains were resistant to several antibiotics, including moxifloxacin (80%), clindamycin (60%) and rifaximin (60%). Moreover, four of five ST1 presented a metronidazole minimum inhibitory concentration of 4 µg/mL. Moxifloxacin resistance was highest (72.3%) for ST11. In conclusion, binary toxin-positive strains are non-prevalent in Korea and involve diverse STs. ST1 strains were resistant to several antibiotics.
Chemical composition of Lavatera thuringiaca L. biomass ash after pre-sowing stimulation of seeds with He–Ne laser light
Authors: Budzeń Małgorzata , Zając Grzegorz , Sujak Agnieszka , Szyszlak-Bargłowicz Joanna , Kafarski Marcin 
Published: January 2023

AbstractThe article presents the effect of pre-sowing Lavatera thuringiaca L. seeds stimulation with He–Ne laser light on the chemical composition (P, S, K, Ca, Mn, Fe, Ni, Cu and Zn content) of ash obtained after combustion of shoots of different ages. Results varied, but it was confirmed that pre-sowing exposure of seeds to this physical factor for 10-min has the most pronounced effect on K, Cu and Mn content increase (6%, 20%, 31% increase respectively) in the ash after the first year of vegetation as well as on Cu, K and Zn content increase (9%, 19%, 22% increase respectively) after the second year of vegetation. However, 30-min stimulation significantly increases Ca (8%), Mn (20%) and Fe (72%) content in ash after the second year as well as results in ash richer in Ca (22%), P (48%), K (70%) and Zn (95%) after the third year of Lavatera vegetation. The pre-sowing application of He–Ne laser light depending on the time of stimulation can intensify the content of preferable macro- and microelement groups in Lavatera ash, in respective cultivation years. It can be an innovative method of biomass ash enhancement and its more effective use in agriculture as commercial fertilizers substitute.
Systemic factors associated with 10-year glaucoma progression in South Korean population: a single center study based on electronic medical records
Authors: Yoon Jung Suk , Kim Ye-eun , Lee Eun Ji , Kim Hyunjoong , Kim Tae-Woo 
Published: January 2023

AbstractGlaucoma is a multifactorial disease where various systemic features are involved in the progression of the disease. Based on initial systemic profiles in electronic medical records, this study aimed to develop a model predicting factors of long-term rapid retinal nerve fiber layer (RNFL) thinning over 5 years in 505 patients with primary open-angle glaucoma. Eyes with faster or slower RNFL thinning were stratified using a decision tree model, and systemic and ophthalmic data were incorporated into the models based on random forest and permutation methods, with the models interpreted by Shapley additive explanation plots (SHAP). According to the decision tree, a higher lymphocyte ratio (> 34.65%) was the most important systemic variable discriminating faster or slower RNFL thinning. Higher mean corpuscular hemoglobin (> 32.05 pg) and alkaline phosphatase (> 88.0 IU/L) concentrations were distinguishing factors in the eyes with lymphocyte ratios > 34.65% and < 34.65%, respectively. SHAP demonstrated larger baseline RNFL thickness, greater fluctuation of intraocular pressure (IOP), and higher maximum IOP as the strongest ophthalmic factors, while higher lymphocyte ratio and higher platelet count as the strongest systemic factors associated with faster RNFL thinning. Machine learning-based modeling identified several systemic factors as well as previously acknowledged ophthalmic risk factors associated with long-term rapid RNFL thinning.
Mechanistic insights into silica nanoparticle–allergen interactions on antigen presenting cell function in the context of allergic reactions
Authors: Johnson Litty , Aglas Lorenz , Punz Benjamin , Dang Hieu-Hoa , Christ Constantin , Pointner Lisa , Wenger Mario , Hofstaetter Norbert , Hofer Sabine , Geppert Mark , Andosch Ancuela , Ferreira Fatima , Horejs-Hoeck Jutta , Duschl Albert , Himly Martin 
Published: January 2023

Impact of SiO2 NP-allergen interaction on dendritic cell function altering the immune response, eventually resulting in a harmless, beneficial outcome in terms of allergic reactivity.
Tuning the 1T′/2H phases in WxMo1−xSe2 nanosheets
Authors: Sokolikova Maria S. , Cheng Gang , Och Mauro , Palczynski Pawel , El Hajraoui Khalil , Ramasse Quentin M. , Mattevi Cecilia 
Published: January 2023

Nanomaterial morphology, chemical composition and 1T′/2H crystal phase tuning is achieved in colloidal WxMo1−xSe2 nanosheets synthesised by a direct colloidal reaction.
Retracted: Multi‐mode urban rail transit and spatial coordinated development based on deep learning system
Authors: Wang Xiaojian , Wang Zixuan , Sun Xiaoye 
Published: January 2023

AbstractRetraction: [Xiaojian Wang, Zixuan Wang, Xiaoye Sun, Multi‐mode urban rail transit and spatial coordinated development based on deep learning system, IET Circuits, Devices & Systems 2023 (https://doi.org/10.1049/cds2.12144)].The above article from IET Circuits, Devices & Systems, published online on 10 January 2023 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the Editor‐in‐Chief, Harry E. Ruda, the Institution of Engineering and Technology (the IET) and John Wiley and Sons Ltd. This article was published as part of a Guest Edited special issue. Following an investigation, the IET and the journal have determined that the article was not reviewed in line with the journal’s peer review standards and there is evidence that the peer revie process of the special issue underwent systematic manipulation. Accordingly, we cannot vouch for the integrity or reliability of the content. As such we have taken the decision to retract the article. The authors have been informed of the decision to retract.
Smartphone sensors‐based human activity recognition using feature selection and deep decision fusion
Authors: Zhang Yijia , Yao Xiaolan , Fei Qing , Chen Zhen 
Published: January 2023

AbstractHuman activity recognition (HAR) with smartphone sensors is a significant research direction in human‐cyber‐physical systems. Aiming at the problem of feature redundancy and low recognition accuracy of HAR, this paper presents a novel system architecture comprising three parts: feature selection based on an oppositional and chaos particle swarm optimization (OCPSO) algorithm, multi‐input one‐dimensional convolutional neural network (MI‐1D‐CNN) utilizing time‐domain and frequency‐domain signals, and deep decision fusion (DDF) combining D‐S evidence theory and entropy. The proposed architecture is evaluated on the UCI HAR and WIDSM datasets. The results highlight that OCPSO performs better than particle swarm optimization (PSO) in feature selection, convergence speed, and recognition accuracy. Moreover, it is shown that for the MI‐1D‐CNN classifier, the frequency‐domain signals (95.96%) perform better than time‐domain signals (95.66%). In addition, this paper investigates the impact of the convolution layers, feature maps, filter sizes, and decision fusion methods on recognition accuracy. The results demonstrate that the DDF method (97.81%) outperforms single‐layer decision fusion in improving the recognition accuracy on the UCI HAR dataset.
A novel smoke detection algorithm based on improved mixed Gaussian and YOLOv5 for textile workshop environments
Authors: Chen Xin , Xue Yipeng , Zhu Yaolin , Ma Ruiqing 
Published: January 2023

AbstractThere are a lot of flammable materials in the textile workshop, and once a fire occurs, it will cause property damage and casualties. At present, smoke detection in textile workshops mainly relies on temperature‐sensing smoke sensors with low detection rate and poor real‐time performance, which cannot meet the task of smoke detection in complex environments. Therefore, this paper proposes an improved mixed Gaussian and YOLOv5 smoke detection algorithm for textile workshops. In order to reduce the interference of static background in smoke detection, an improved gaussian mixture algorithm is used to extract suspected smoke areas in video by using the dynamic characteristics of smoke. Then, an adaptive attention module is added to the feature pyramid infrastructure of the YOLOv5 target detection network to improve the multi‐scale target recognition ability. In addition, the focal loss function is used to reduce the impact of background and foreground class imbalances on the detection results. The experimental results show that the detection accuracy of the proposed method is 94.7%, and the average detection speed is 66.7 FPS. By comparing with the existing state‐of‐the‐art algorithms, the detection capability of this method has been significantly improved. At the same time, it has high real‐time performance and detection accuracy in smoke detection in textile workshops.
The Beauty of Musculoskeletal Ultrasound: Spot Diagnoses
Authors: Drakonaki Elena E , Martinoli Carlo , Vanhoenacker Filip Maria , Detoraki Anna , Dalili Daniel E , Adriaensen Miraude 
Published: January 2023

Introduction Ultrasound is a powerful technique in musculoskeletal (MSK) imaging, and it can replace MR imaging in many specific clinical scenarios. This article will feature some common and less common spot diagnoses in musculoskeletal ultrasound.
Spot diagnosis Cases were collected by members of the Educational Committee of the ESSR ( European Society of Musculoskeletal Radiology) with expertise in musculoskeletal ultrasound. Sixteen clinical entities are discussed based on the features that allow US spot diagnosis.
Conclusion Clinical history, location, and ultrasound appearance are the keys to spot diagnoses when performing musculoskeletal ultrasound.
Key Points:
Citation Format