bluuebunny/crossref_metadata_2025_split huggingface dataset:
NHC-catalyzed enantioselective access to β-cyano carboxylic esters via in situ substrate alternation and release
Authors: Wang Qingyun , Wu Shuquan , Zou Juan , Liang Xuyang , Mou Chengli , Zheng Pengcheng , Chi Yonggui Robin 
Published: August 2023

AbstractA carbene-catalyzed asymmetric access to chiral β-cyano carboxylic esters is disclosed. The reaction proceeds between β,β-disubstituted enals and aromatic thiols involving enantioselective protonation of enal β-carbon. Two main factors contribute to the success of this reaction. One involves in situ ultrafast addition of the aromatic thiol substrates to the carbon-carbon double bond of the enal substrate. This reaction converts almost all enal substrate to a Thiol-click Intermediate, significantly reducing aromatic thiol substrates concentration and suppressing the homo-coupling reaction of enals. Another factor is an in situ release of enal substrate from the Thiol-click Intermediate for the desired reaction to proceed effectively. The optically enriched β-cyano carboxylic esters from our method can be readily transformed to medicines that include γ-aminobutyric acids derivatives such as Rolipram. In addition to synthetic utilities, our control of reaction outcomes via in situ substrate modulation and release can likely inspire future reaction development.
Pb-rich Cu grain boundary sites for selective CO-to-n-propanol electroconversion
Authors: Niu Wenzhe , Chen Zheng , Guo Wen , Mao Wei , Liu Yi , Guo Yunna , Chen Jingzhao , Huang Rui , Kang Lin , Ma Yiwen , Yan Qisheng , Ye Jinyu , Cui Chunyu , Zhang Liqiang , Wang Peng , Xu Xin , Zhang Bo 
Published: August 2023

AbstractElectrochemical carbon monoxide (CO) reduction to high-energy-density fuels provides a potential way for chemical production and intermittent energy storage. As a valuable C3 species, n-propanol still suffers from a relatively low Faradaic efficiency (FE), sluggish conversion rate and poor stability. Herein, we introduce an “atomic size misfit” strategy to modulate active sites, and report a facile synthesis of a Pb-doped Cu catalyst with numerous atomic Pb-concentrated grain boundaries. Operando spectroscopy studies demonstrate that these Pb-rich Cu-grain boundary sites exhibit stable low coordination and can achieve a stronger CO adsorption for a higher surface CO coverage. Using this Pb-Cu catalyst, we achieve a CO-to-n-propanol FE (FEpropanol) of 47 ± 3% and a half-cell energy conversion efficiency (EE) of 25% in a flow cell. When applied in a membrane electrode assembly (MEA) device, a stable FEpropanol above 30% and the corresponding full-cell EE of over 16% are maintained for over 100 h with the n-propanol partial current above 300 mA (5 cm2 electrode). Furthermore, operando X-ray absorption spectroscopy and theoretical studies reveal that the structurally-flexible Pb-Cu surface can adaptively stabilize the key intermediates, which strengthens the *CO binding while maintaining the C–C coupling ability, thus promoting the CO-to-n-propanol conversion.
Multicolor lifetime imaging and its application to HIV-1 uptake
Authors: Starling Tobias , Carlon-Andres Irene , Iliopoulou Maro , Kraemer Benedikt , Loidolt-Krueger Maria , Williamson David J. , Padilla-Parra Sergi 
Published: August 2023

AbstractSimultaneous imaging of nine fluorescent proteins is demonstrated in a single acquisition using fluorescence lifetime imaging microscopy combined with pulsed interleaved excitation of three laser lines. Multicolor imaging employing genetically encodable fluorescent proteins permits spatio-temporal live cell imaging of multiple cues. Here, we show that multicolor lifetime imaging allows visualization of quadruple labelled human immunodeficiency viruses on host cells that in turn are also labelled with genetically encodable fluorescent proteins. This strategy permits to simultaneously visualize different sub-cellular organelles (mitochondria, cytoskeleton, and nucleus) during the process of virus entry with the potential of imaging up to nine different spectral channels in living cells.
Rapid transition from primary to secondary crust building on the Moon explained by mantle overturn
Authors: Prissel Tabb C. , Zhang Nan , Jackson Colin R. M. , Li Haoyuan 
Published: August 2023

AbstractGeochronology indicates a rapid transition (tens of Myrs) from primary to secondary crust building on the Moon. The processes responsible for initiating secondary magmatism, however, remain in debate. Here we test the hypothesis that the earliest secondary crust (Mg-suite) formed as a direct consequence of density-driven mantle overturn, and advance 3D mantle convection models to quantify the resulting extent of lower mantle melting. Our modeling demonstrates that overturn of thin ilmenite-bearing cumulates ≤ 100 km triggers a rapid and short-lived episode of lower mantle melting which explains the key volume, geochronological, and spatial characteristics of early secondary crust building without contributions from other energy sources, namely KREEP (potassium, rare earth elements, phosphorus, radiogenic U, Th). Observations of globally distributed Mg-suite eliminate degree-1 overturn scenarios. We propose that gravitational instabilities in magma ocean cumulate piles are major driving forces for the onset of mantle convection and secondary crust building on differentiated bodies.
Nonlinearity of the cloud response postpones climate penalty of mitigating air pollution in polluted regions
Authors: Jia Hailing , Quaas Johannes 
Published: August 2023

AbstractAerosol–cloud interactions contribute substantially to uncertainties in anthropogenic forcing, in which the sensitivity of cloud droplet number concentration (Nd) to aerosol plays a central role. Here we use satellite observations to show that the aerosol–Ndrelation (in log–log space) is not linear as commonly assumed. Instead, theNdsensitivity decreases at large aerosol concentrations due to the transition from aerosol-limited to updraft-limited regime, making the widely used linear method problematic. A sigmoidal transition is shown to adequately fit the data. When using this revised relationship, the additional warming that arises from air pollution mitigation is delayed by two to three decades in heavily polluted locations, compared to the linear relationship. This cloud-mediated climate penalty will manifest markedly starting around 2025 in China and 2050 in India after applying the strongest air quality policy, underlining the urgency of mitigating greenhouse gas emissions.
Improved fluoroscopy-guided biopsies in the diagnosis of indeterminate biliary strictures: a multi-center retrospective study
Authors: Xiong Zhe , Wang Kuangjing , Zhang Huahui , Fang Ying , Li Fengdong , Huang Jin 
Published: August 2023

AbstractTo evaluate the diagnostic accuracy of improved fluoroscopy-guided biopsies for indeterminate biliary strictures (IBDS). A multi-center retrospective study was performed. Patients with IBDS who underwent digital single-operator cholangioscopy (DSOC) and improved fluoroscopy-guided biopsies procedures were included. The individual sensitivity, specificity, and accuracy were analyzed. A total of 67 patients were enrolled in this multi-center retrospective study. The DSOC and improved fluoroscopy-guided biopsies procedures were successfully performed in all cases (100%). The sensitivity, specificity, and accuracy values were 83.3%, 89.5%, and 85.1% for DSOC visual impression; 95.8%, 94.7%, and 95.5% for improved fluoroscopy-guided biopsies procedures, respectively. The sensitivity and accuracy of improved fluoroscopy-guided biopsies were significantly higher compared with DSOC visual impression. Four patients (6.0%, 4/67) occurred adverse events after the procedures. Improved fluoroscopy-guided biopsies had a high diagnostic accuracy of IBDS diagnosis.
Central nervous system biomarkers GFAp and NfL associate with post-acute cognitive impairment and fatigue following critical COVID-19
Authors: Bark Lovisa , Larsson Ing-Marie , Wallin Ewa , Simrén Joel , Zetterberg Henrik , Lipcsey Miklos , Frithiof Robert , Rostami Elham , Hultström Michael 
Published: August 2023

AbstractA high proportion of patients with coronavirus disease 2019 (COVID-19) experience post-acute COVID-19, including neuropsychiatric symptoms. Objective signs of central nervous system (CNS) damage can be investigated using CNS biomarkers such as glial fibrillary acidic protein (GFAp), neurofilament light chain (NfL) and total tau (t-tau). We have examined whether CNS biomarkers can predict fatigue and cognitive impairment 3–6 months after discharge from the intensive care unit (ICU) in critically ill COVID-19 patients. Fifty-seven COVID-19 patients admitted to the ICU were included with analysis of CNS biomarkers in blood at the ICU and at follow up. Cognitive dysfunction and fatigue were assessed with the Montreal Cognitive Assessment (MoCA) and the Multidimensional Fatigue inventory (MFI-20). Elevated GFAp at follow-up 3–6 months after ICU discharge was associated to the development of mild cognitive dysfunction (p = 0.01), especially in women (p = 0.005). Patients who experienced different dimensions of fatigue at follow-up had significantly lower GFAp in both the ICU and at follow-up, specifically in general fatigue (p = 0.009), physical fatigue (p = 0.004), mental fatigue (p = 0.001), and reduced motivation (p = 0.001). Women showed a more pronounced decrease in GFAp compared to men, except for in mental fatigue where men showed a more pronounced GFAp decrease compared to women. NfL concentration at follow-up was lower in patients who experienced reduced motivation (p = 0.004). Our findings suggest that GFAp and NfL are associated with neuropsychiatric outcome after critical COVID-19.Trial registration The study was registered à priori (clinicaltrials.gov: NCT04316884 registered on 2020-03-13 and NCT04474249 registered on 2020-06-29).
The effects of the NMDAR co-agonist d-serine on the structure and function of optic tectal neurons in the developing visual system
Authors: Chorghay Zahraa , Li Vanessa J. , Schohl Anne , Ghosh Arna , Ruthazer Edward S. 
Published: August 2023

AbstractThe N-methyl-d-aspartate type glutamate receptor (NMDAR) is a molecular coincidence detector which converts correlated patterns of neuronal activity into cues for the structural and functional refinement of developing circuits in the brain. d-serine is an endogenous co-agonist of the NMDAR. We investigated the effects of potent enhancement of NMDAR-mediated currents by chronic administration of saturating levels of d-serine on the developing Xenopus retinotectal circuit. Chronic exposure to the NMDAR co-agonist d-serine resulted in structural and functional changes in the optic tectum. In immature tectal neurons, d-serine administration led to more compact and less dynamic tectal dendritic arbors, and increased synapse density. Calcium imaging to examine retinotopy of tectal neurons revealed that animals raised in d-serine had more compact visual receptive fields. These findings provide insight into how the availability of endogenous NMDAR co-agonists like d-serine at glutamatergic synapses can regulate the refinement of circuits in the developing brain.
Distinction between rhinitis alone and rhinitis with asthma using interactomics
Authors: Aguilar Daniel , Lemonnier Nathanaël , Melén Erik , Bustamante Mariona , Gruzieva Olena , Guerra Stefano , Keil Thomas , Koppelman Gerard H. , Celedón Juan C. , Antó Josep M. , Bousquet Jean 
Published: August 2023

AbstractThe concept of “one-airway-one-disease”, coined over 20 years ago, may be an over-simplification of the links between allergic diseases. Genomic studies suggest that rhinitis alone and rhinitis with asthma are operated by distinct pathways. In this MeDALL (Mechanisms of the Development of Allergy) study, we leveraged the information of the human interactome to distinguish the molecular mechanisms associated with two phenotypes of allergic rhinitis: rhinitis alone and rhinitis in multimorbidity with asthma. We observed significant differences in the topology of the interactomes and in the pathways associated to each phenotype. In rhinitis alone, identified pathways included cell cycle, cytokine signalling, developmental biology, immune system, metabolism of proteins and signal transduction. In rhinitis and asthma multimorbidity, most pathways were related to signal transduction. The remaining few were related to cytokine signalling, immune system or developmental biology. Toll-like receptors and IL-17-mediated signalling were identified in rhinitis alone, while IL-33 was identified in rhinitis in multimorbidity. On the other hand, few pathways were associated with both phenotypes, most being associated with signal transduction pathways including estrogen-stimulated signalling. The only immune system pathway was FceRI-mediated MAPK activation. In conclusion, our findings suggest that rhinitis alone and rhinitis and asthma multimorbidity should be considered as two distinct diseases.
Clinical significance of the expression of FOXP3 and TIGIT in Merkel cell carcinoma
Authors: Iwasaki Takeshi , Hayashi Kazuhiko , Matsushita Michiko , Nonaka Daisuke , Matsumoto Takamasa , Taniguchi Midori , Kuwamoto Satoshi , Umekita Yoshihisa , Oda Yoshinao 
Published: August 2023

AbstractThe pathogenesis of 80% of Merkel cell carcinoma (MCC) cases is associated with Merkel cell polyomavirus (MCPyV). Forkhead helix transcription factor P3 (FOXP3) and the T cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibition motif domains (TIGIT)–CD155 pathway, which are targets for immunotherapy, were assessed as prognostic factors of MCC. We analyzed mRNA expression data of 111 patients with MCC and performed immunohistochemical analysis to detect the expression of programmed death ligand 1 (PD-L1), CD8, FOXP3, TIGIT, and CD155 in 65 cases of MCC. In CD8 and FOXP3 immunostaining, the number of expressing-infiltrating cells was determined by dividing the region into tumor center and invasive front areas. FOXP3 expression was evaluated separately in cells with high and low intensities. Aberrant TIGIT expression and weak CD155 staining were observed in MCC cells. CD8- and FOXP3-positive cell infiltrations were higher in the invasive front than in the tumor center. Multivariate Cox hazard analysis revealed that high infiltration of cells with low-intensity FOXP3 expression in the invasive front is a favorable prognostic factor (p = 0.025). Thus, targeting TIGIT–CD155 signaling and FOXP3 as well as PD-L1 may be a therapeutic strategy for MCC.
Soluble HLA-G (sHLA-G) measurement might be useful as an early diagnostic biomarker and screening test for gastric cancer
Authors: Mejía-Guarnizo Lidy Vannessa , Monroy-Camacho Paula Stefanny , Rincón-Rodríguez Daniel Esteban , Rincón-Riveros Andrés , Martinez-Vargas Daniel Augusto , Huertas-Caro Carlos Alexander , Oliveros-Wilches Ricardo , Sanchez-Pedraza Ricardo , Nuñez-Lemus Marcela , Cristancho-Lievano Carlos Felipe , Castellanos-Moreno Adriana Milena , Martinez-Correa Lina María , Rodríguez-García Josefa Antonia 
Published: August 2023

AbstractGastric cancer (GC) is the fifth most frequent malignancy worldwide and has a high mortality rate related to late diagnosis. Although the gold standard for the GC diagnosis is endoscopy with biopsy, nonetheless, it is not cost-effective and is invasive for the patient. The Human leukocyte antigen G (HLA-G) molecule is a checkpoint of the immune response. Its overexpression in cancer is associated with immune evasion, metastasis, poor prognosis, and lower overall survival. We evaluate the plasma levels of soluble HLA-G, (sHLA-G) in patients with GC and benign gastric pathologies using an ELISA test. A higher concentration of sHLA-G in patients with GC than in those with benign pathologies, higher levels of plasma sHLA-G in women with GC compared with men and significant differences in the sHLA-G levels between the benign gastric pathologies evaluated, was our main findings. As no significant differences were found between the GC assessed stages in our study population, we suggest that sHLA-G is not an adequate marker for staging GC, but it does have diagnostic potential. In addition to providing information on the potential of sHLA-G as a diagnostic marker for GC, our study demonstrate that HLA-G molecules can be found in the membrane of exosomes, which highlights the need to perform studies with a larger number of samples to explore the functional implications of HLA-G positive exosomes in the context of gastric cancer, and to determine the clinical significance and possible applications of these findings in the development of non-invasive diagnostic methods.
Using heterogeneous sources of data and interpretability of prediction models to explain the characteristics of careless respondents in survey data
Authors: Kopitar Leon , Stiglic Gregor 
Published: August 2023

AbstractPrior to further processing, completed questionnaires must be screened for the presence of careless respondents. Different people will respond to surveys in different ways. Some take the easy path and fill out the survey carelessly. The proportion of careless respondents determines the survey’s quality. As a result, identifying careless respondents is critical for the quality of obtained results. This study aims to explore the characteristics of careless respondents in survey data and evaluate the predictive power and interpretability of different types of data and indices of careless responding. The research question focuses on understanding the behavior of careless respondents and determining the effectiveness of various data sources in predicting their responses. Data from a three-month web-based survey on participants’ personality traits such as honesty-humility, emotionality, extraversion, agreeableness, conscientiousness and openness to experience was used in this study. Data for this study was taken from Schroeders et al.. The gradient boosting machine-based prediction model uses data from the answers, time spent for answering, demographic information on the respondents as well as some indices of careless responding from all three types of data. Prediction models were evaluated with tenfold cross-validation repeated a hundred times. Prediction models were compared based on balanced accuracy. Models’ explanations were provided with Shapley values. Compared with existing work, data fusion from multiple types of information had no noticeable effect on the performance of the gradient boosting machine model. Variables such as “I would never take a bribe, even if it was a lot”, average longstring, and total intra-individual response variability were found to be useful in distinguishing careless respondents. However, variables like “I would be tempted to use counterfeit money if I could get away with it” and intra-individual response variability of the first section of a survey showed limited effectiveness. Additionally, this study indicated that, whereas the psychometric synonym score has an immediate effect and is designed with the goal of identifying careless respondents when combined with other variables, it is not necessarily the optimal choice for fitting a gradient boosting machine model.
Effect of temperature on the dynamic parameters of silty clay in a seasonally frozen region
Authors: Guo Haotian , Lin Yuli , Sun Chao , Mao Xin , Li Jinfeng 
Published: August 2023

AbstractThe effect of temperature on the dynamic parameters of silty clay in a seasonally frozen region was assessed using a GDS dynamic triaxial test system. The strength parameters, dynamic elastic modulus, damping ratio, and other dynamic parameters of the soil samples were analyzed under different temperature conditions. The results demonstrated that the shear strength parameters (internal friction angle and cohesion) of the silty clay under a dynamic load increased significantly with decreasing temperature, and the internal friction angle increased sharply below 0 °C. The dynamic elastic modulus increased as the temperature decreased and changed significantly in the ice-water phase change region. The slope of the dynamic stress–strain curve of the soil sample increased significantly with decreasing temperature. As the temperature decreased, the damping ratio reduced, and the ability of the soil to absorb seismic waves declines. The research results provide new data and information to guide construction projects in seasonally frozen region.
Identification of LncPVT1 and CircPVT1 as prognostic biomarkers in human colorectal polyps
Authors: RezaSoltani Mahsa , Forouzesh Flora , Salehi Zahra , Zabihi Mohammad-Reza , Rejali Leili , Nazemalhosseini-Mojarad Ehsan 
Published: August 2023

AbstractLncPVT1 and CircPVT1 are isoforms for the PVT1 gene and are associated with cancer progression and carcinogenesis. Our study investigated the expression of LncPVT1 and CircPVT1 in colon adenoma polyps. 40 tissues of colorectal polyps and 40 normal-adjacent tissues (NATs) were taken. The expression of LncPVT1 and CircPVT1 was evaluated through qRael-Time PCR. The relation between expression and features of clinicopathological was explored. The ceRNA network was constructed by LncPVT1 and CircPVT1 and predicted miRNAs and miRNAs targets. Further, hub nodes in this network were determined using the cytoHubba package. Over-expressed LncPVT1 and CircPVT1 were differentiated in polyp and NATs. The expression level of LncPVT1 and CircPVT1 were significantly higher in adenoma polyps than in hyperplastic polyps. The area under the curve of the ROC estimate for the LncPVT1 and CircPVT1 was 0.74 and 0.77, respectively. A positive correlation was observed between the LncPVT1 expression and CircPVT1. Three miRNAs, including hsa-miR-484, hsa-miR-24-3p, hsa-miR-423-5p, and CircPVT1, were detected as ceRNA hub nodes. In this study, expression profiles of LncPVT1 and CircPVT1 were significantly higher in precancerous polyps. In addition, based on our in silico analysis, LncPVT1, CircPVT1/miR-484, miR-24-3p, miR-423-5p/PLAGL2 axis might be involved in colon cancer development. LncPVT1 and CircPVT1 can be prescribed as warning problems as potential prognostic biomarkers in patients with pre-CRC colon polyps.