bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroendocrine neoplasm of the gallbladder: Clinical features, surgical efficacy, and prognosis
Authors: Wu Xin , Li Binglu , Hong Tao , Liu Wei , He Xiaodong , Zheng Chaoji 
Published: March 2023

AbstractBackgroundNeuroendocrine neoplasm (NEN) of the gallbladder is rare. It is usually asymptomatic and occurs in older adults. Its clinicopathological characteristics remain controversial, and the diagnosis and treatment strategies are usually based on models of adenocarcinoma. The present study aimed to investigate the clinical characteristics and prognosis of gallbladder NEN.MethodsThe data of patients with gallbladder NEN admitted to Peking Union Medical College Hospital was reviewed, and a database was established for retrospective analysis. Clinicopathological features were analyzed descriptively and the prognosis was studied according to different factors. The Kaplan–Meier curve was used to describe the cumulative survival rate.ResultsIn total, 22 patients with gallbladder NEN were included in this study. There were 10 male (45.5%) and 12 female (54.5%) patients with a median age of onset of NEN at 57.5 (49.0, 62.3) years. Abdominal discomfort was the most common symptom. Twenty patients (90.9%) underwent surgery, and two patients (9.1%) with unresectable lesions underwent a biopsy. Twenty‐one patients were followed up. The 1‐, 2‐, and 3‐year cumulative overall survival rates of all patients and patients with resectable lesions were 65.9%, 54.9%, and 48.1%, and 72.9%, 60.7%, and 53.1%, respectively. Patients with resectable lesions had a better cumulative overall survival rate than those who with unresectable lesions (p < 0.001).ConclusionGallbladder NEN is more common in the elderly and has a slight female predominance. The most common symptom is abdominal discomfort. Surgery is the first choice of treatment for this rare disease. The prognosis of gallbladder NEN is generally poor. Patients with resectable lesions have a better prognosis.
The nature of deprovincialism: Assessment, nomological network, and comparison of cultural and group deprovincialization
Authors: Lucarini Alice , Boin Jessica , Fuochi Giulia , Voci Alberto , Verkuyten Maykel , Pettigrew Thomas F. 
Published: March 2023

AbstractThis study (N = 770) explores in depth the construct of deprovincialization by both uniting and comparing two scales that assess its two facets: the Group Deprovincialization Scale (GDS; Martinovic & Verkuyten, 2013) and the Cultural Deprovincialization Scale (CDS, Boin et al., 2020). First, we tested the factorial structure of the construct through confirmatory factor analyses. Second, we compared the mean scores of the GDS and the CDS and a list of variables related to individual dispositions and intergroup outcomes for participants who had (vs. had not) lived abroad. Then, we explored the nomological net of correlates of deprovincialization to examine whether the GDS and the CDS differed in their relationship with the correlates. Finally, we tested the simultaneous relationships of both scales with a subset of variables via network analysis. Results offer insights on the important construct of deprovincialization, its assessment, and the relevance of its facets, showing that the GDS and te CDS tap into related yet different nuances of the broad deprovincialization construct. Please refer to the Supplementary Material section to find this article's Community and Social Impact Statement.
Implicit smoothed particle hydrodynamics model for simulating incompressible fluid‐elastic coupling
Authors: Wang Xiaokun , Wang Tiancheng , Wang Jiamin , Xu Yanrui , Ban Xiaojuan , Huang Houbin , Zhu Zhihong , Chang Jian , Zhang Jian Jun 
Published: March 2023

AbstractFluid simulation has been one of the most critical topics in computer graphics for its capacity to produce visually realistic effects. The intricacy of fluid simulation manifests most with interacting dynamic elements. The coupling for such scenarios has always been challenging to manage due to the numerical instability arising from the coupling boundary between different elements. Therefore, we propose an implicit smoothed particle hydrodynamics fluid‐elastic coupling approach to reduce the instability issue for fluid‐fluid, fluid‐elastic, and elastic‐elastic coupling circumstances. By deriving the relationship between the universal pressure field with the incompressible attribute of the fluid, we apply the number density scheme to solve the pressure Poisson equation for both fluid and elastic material to avoid the density error for multi‐material coupling and conserve the non‐penetration condition for elastic objects interacting with fluid particles. Experiments show that our method can effectively handle the multiphase fluids simulation with elastic objects under various physical properties.
Influence factors of channel geometry for separation of circulating tumor cells by four‐ring inertial focusing microchannel
Authors: Liu Donglei , Chen Song , Luo Xin 
Published: March 2023

AbstractInertial microfluidics is a high‐throughput and high‐efficiency cell separation approach to which attention has been progressively paid in recent years. However, research on the influencing factors that compromise the efficiency of cell separation is still lacking. Therefore, the aim of this study was to evaluate the cell separation efficiency by changing the influencing factors. A four‐ring inertial focusing spiral microchannel was designed to separate two kinds of circulating tumor cells (CTCs) from blood. Human breast cancer (MCF‐7) cells and human epithelial cervical cancer (HeLa) cells enter the four‐ring inertial focusing spiral microchannel together with blood cells, and cancer cells and blood cells were separated from each other at the outlet of the channel by inertial force. The cell separation efficiency at the inlet flow rate in the Reynolds number range of 40–52 was studied by changing the influencing factors such as the cross‐sectional shape of the microchannel, the average thickness of the cross‐section, and the trapezoidal inclination angle. The results showed that the reduction of the channel thickness and the increase of a certain trapezoidal inclination enhanced the cell separation efficiency to a certain extent, the study showed that when the channel inclination was 6 ° and the average channel thickness was 160 μm. The two kinds of CTC cells could be completely separated from the blood and the efficiency could reached 100%.
Definition of an Index Parameter To Screen Highly Functional Enzymes Derived from a Biochemical and Thermodynamic Analysis of Ancestral meso‐Diaminopimelate Dehydrogenases
Authors: Araseki Hayato , Kawasaki Mayu , Kambe Akira , Kozuka Kohei , Ito Sohei , Nakano Shogo 
Published: March 2023

AbstractSequence‐based protein design approaches are being adopted to generate highly functional enzymes; however, screening the enzymes remains a time‐consuming task. In this study, by analyzing the enzymatic properties of four ancestral meso‐2,6‐diaminopimelate dehydrogenases (AncDAPDHs), AncDAPDH‐N1, ‐N2, ‐N3, and ‐N4, we attempted to define a new index parameter that is helpful for efficiently screening the enzymes. Biochemical and thermodynamic analyses indicated that only AncDAPDH‐N4 exhibited greater thermal stability than and activity similar to those of native DAPDHs. Structural and sequence comparisons between DAPDH from Corynebacterium glutamicum (CgDAPDH) and the AncDAPDHs suggested that “quality of mutations” is a potential index parameter. In fact, the mutations introduced from CgDAPDH to AncDAPDH‐N4 correlated highly with the mutations accumulated during the evolution process from mesophiles to thermophiles. These results suggest that, although there are several exceptions, the correlation coefficient can be used as an index parameter for screening high‐functioning enzymes from sequence data.
RNF150 suppresses papillary thyroid carcinoma with ASK1 ubiquitination presenting a direct target via inactivating p38 signaling axis
Authors: Deng Wei , Wu Jing , Zheng Wei , Wang Qian , Li Dongling , Kuang Hongyu 
Published: March 2023

AbstractPapillary thyroid carcinoma (PTC) is the most prevalent cancer in endocrine system. However, the pathogenic mechanism underlying tumor recurrence remains unclear. RING finger protein (RNF) family plays a crucial role in cancers whereas the role of RNF150 in PTC requires investigation. Our study aimed to explore the function and molecular mechanism of RNF150 in PTC. Here, we extracted data from The Cancer Genome Atlas‐THCA (TCGA‐THCA) data set to investigate the expression and prognostic value of RNF150 in THCA. We found that RNF150 was lowly expressed in THCA and its high expression indicated favorable prognosis in THCA patients. RNF150 could inhibit the proliferation of PTC cells and suppress p38 phosphorylation both in vitro and in vivo. Meanwhile, the knockdown of RNF150 significantly promoted the proliferation of PTC cells and the phosphorylation of p38, which could be reversed by p38 inhibitor. Besides, RNF150 could interact with ASK1 and promoted its ubiquitination. The overexpression of ASK1 exerted opposite effects against RNF150 in PTC cells. In PTC specimens, RNF150 and ASK1 shared reversed expression pattern. In conclusion, our study revealed that RNF150 could suppress the proliferation of PTC by inactivating p38 pathway and promoting ASK1 ubiquitination, which provided novel targets for PTC treatment.
Plaque characterization of a saphenous vein graft by near‐infrared spectroscopy and histopathology in a patient with a percutaneous coronary intervention
Authors: Mori Hiroki , Kurita Tairo , Takasaki Akihiro , Dohi Kaoru 
Published: March 2023

AbstractNear‐infrared spectroscopy (NIRS) is an intravascular imaging (IVUS) modality that detects lipid core plaques in the vessel wall, which are regarded as high‐risk plaques for distal embolization in percutaneous coronary interventions (PCI). Saphenous vein graft (SVG) lesions have friable lipid‐rich plaques and thrombus prone to distal embolization. The plaque characterization of SVG by NIRS was confirmed herein for the first time with histopathology before and after PCI. The present case was a man in his 60 s with a history of coronary artery bypass graft surgery. Coronary angiography revealed severe stenosis in multiple segments of the SVG to left circumflex artery (LCX). NIRS IVUS showed large amounts of lipidic materials at each segment before PCI. After balloon dilatation, NIRS IVUS revealed a marked reduction in yellow signals on chemography. A histopathological analysis of the captured specimen showed that it was mainly composed of fibrin and contained numerous histiocytes with foam cells, lymphocytes, and other cells, which was consistent with the histopathological findings of plaque rupture.
Octahedral Iron‐Acyl‐Nitrenoid Intermediates in Sulphur −Nitrogen Coupling and Hydrogen Atom Transfer Reactions
Authors: Rodríguez Mònica , Pereverzev Aleksandr Y. , Roithová Jana 
Published: March 2023

AbstractIron‐nitrenoid complexes can activate C−H bonds and, thus, potentially directly transform C−H into C−N bonds. Low‐coordination complexes are the main reported systems; however, octahedral iron complexes can be air‐stable alternatives forming the iron‐nitrenoid species. Here, a novel octahedral non‐heme iron‐acyl‐nitrenoid complex is presented. Its reactivity was studied using a flow chemistry setup with online mass spectrometry detection, which allows qualitative reaction kinetics evaluation and the detection of the intermediates. The iron‐nitrenoid complex reacts with thiophenol by H‐Atom transfer, followed by a rebound to form a new N−S bond. The rebound intermediate could be detected and distinguished from the product complex. The reaction of the iron‐acyl‐nitrenoid complex with hydrocarbons results in the activation of the C−H bond via hydrogen‐atom transfer with no subsequent rebound process.
Chiral Diketopyrrolo[3,4‐c]pyrrole‐1,2,3‐1H‐triazole Dyes with Highly Tuneable Properties in Solution and Thin Films
Authors: Albano Gianluigi , Zinna Francesco , Taddeucci Andrea , Capozzi Maria Annunziata M. , Pescitelli Gennaro , Punzi Angela , Di Bari Lorenzo , Farinola Gianluca M. 
Published: March 2023

AbstractWe have studied the impact of achiral substituents on the chiral supramolecular architectures of diketopyrrolo[3,4‐c]pyrrole‐1,2,3‐1H‐triazole (DPP) dyes. We decorated the same chiral DPP motif with substituent groups on the nitrogen atoms of the lactam moiety: the hydrophobic n‐octyl alkyl chain, the hydrophilic tri(ethylene glycol) (TEG) chain and the thermo‐cleavable tert‐butoxycarbonyl (t‐Boc) carbamate group. In spite of having identical conjugated chromophore and chiral appendages, in aggregated form the three dyes displayed profoundly different optical, chiroptical, electrochemical and thermal features. ECD measurements revealed differences in the aggregation modes, which would be inaccessible by most other techniques. We found strong chiroptical features, which would have major implications in the context of chiral organic opto‐electronics and in the development of other highly innovative technological applications.
Modelling of a continuous kneader reactor for the polymerization of partially neutralized acrylic acid
Authors: Luciani Carla , Choi Kyu Yong 
Published: March 2023

AbstractA mathematical model and analysis of the continuous polymerization of partially neutralized acrylic acid (AA) in a continuous kneader reactor is presented here as an initial attempt to simulate the synthesis of a superabsorbent polymer. A detailed kinetic model has been used to describe the copolymerization of AA and sodium acrylate (NaA) in aqueous medium. This model is used to describe batch and continuous operations. The polymerization is initiated by a mixture of potassium persulphate (K2S2O8, KPS) and hydrogen peroxide (H2O2) as oxidizing agent and ascorbic acid (AsA) as reducing agent. A novel set of kinetic parameters has been estimated by fitting experimental data from different literature sources. The operation of a continuous kneader reactor modelled as a plug‐flow reactor with axial dispersion is theoretically investigated to predict temperature profile, total and individual monomer conversion, consumption of KPS, H2O2, and AsA, and polymer average molecular weights. The simulation results show the presence of a hot spot close to the reactor entrance that could be potentially severe during startup and could have a detrimental impact on polymer quality. This model is a first step in the direction of achieving optimal operating protocols and exploring improved polymerization reactor designs.
Radiofrequency ablation of premature ventricular contractions guided by robotic magnetic navigation combined with pattern matching filter
Authors: Liu Xiao‐yu , Zheng Jie , Li Ku‐lin , Dang Shi‐peng , Li Xiao‐yan , Zhao Xiao‐xi , Wang Chao , Yu Zhi‐ming , Wang Ru‐xing 
Published: March 2023

AbstractBackgroundThis study's intent is to evaluate the usefulness of pattern matching filter (PMF) function combined with robotic magnetic navigation (RMN) in guiding the ablation of premature ventricular contractions (PVCs).HypothesisAssume that PMF can improve the outcomes of PVCs ablation using RMN.MethodsA retrospective analysis was completed consisting of 118 consecutive patients with PVCs who underwent radiofrequency ablation guided by RMN. According to the application of PMF, patients were divided into two groups: 20 patients underwent ablation without PMF (group A), and another 98 patients received ablation incorporating PMF (group B).ResultsCompared with group A, the procedure time (135.0 ± 28.3 min vs. 106.3 ± 37.9 min, p = 0.02) in group B was significantly decreased, while the X‐ray exposure time (6.0 ± 2.6 min vs. 6.5 ± 3.6 min, p = 0.705) and dose (3.2 ± 2.4 gycm2 vs. 3.9 ± 2.7 gycm2，p = 0.208) had no significant difference. Group B had a more than twofold number of points acquired (66.9 ± 23.0 vs. 143.9 ± 68.3, p < 0.001) and required a shorter radiofrequency ablation time (13.2 ± 3.5 min vs. 8.1 ± 2.9 min, p < 0.001). There were no serious complications in either group. The acute success rate was similar [90.0% (18/20) vs. 87.8% (86/98), p = 1.000] in two groups, and the success rate was also similar in the long‐term follow‐up [83.3% (15/18) vs. 87.2% (75/86), p = 0.776].ConclusionsThe ablation of PVCs guided by RMN is safe and effective. Combined with the functional capability of PMF, both procedure time and radiofrequency ablation time were significantly decreased.
A cost‐efficient and QoS‐aware adaptive placement of applications in fog computing
Authors: Li Hongjian , Xu Chen , Wang Tiantian , Wang Jingjing , Zheng Peng , Liu Tongming , Tang Libo 
Published: March 2023

SummaryWith the rapid development of the Internet of Things (IoT), fog computing has emerged as a complementary solution to address the issues faced in cloud computing. However, it is a challenging issue to ensure both of the high Quality of Service (QoS) and low cost for different requests when dealing with computing resources. In this article, we propose a new approach on adaptive cost‐efficient and QoS‐aware application placement for fog computing called DATSS. Specifically, this approach consists of a QoS state driven strategy and a credibility rating mechanism. The algorithm divides the QoS into three different states by calculating the QoS violation rate and adaptively selects the best scheduling strategy. Then evaluates the stability of the node based on the task history, and prioritize the task on the node with a higher rating. Performance results show that the cost, delay and energy consumption of the proposed algorithm can be reduced by 20.9%, 15.7%, and 12.6% respectively compared with other algorithms at most. The proposed algorithm can effectively improve cost efficiency and stability under QoS constraints in fog computing environments.
Heteronuclear Trimetallic MFe2 and M2Fe (M=V, Nb, and Ta) Clusters for Dinitrogen Activation
Authors: Wang Ya Ya , Ding Xun‐Lei , Ji Zhi‐Wen , Huang Xiao‐Meng , Li Wei 
Published: March 2023

AbstractCatalysts with heteronuclear metal active sites may have high performance in the nitrogen reduction reaction (NRR), and the in‐depth understanding of the reaction mechanisms is crucial for the design of related catalysts. In this work, the dissociative adsorption of N2 on heteronuclear trimetallic MFe2 and M2Fe (M=V, Nb, and Ta) clusters was studied with density functional theory calculations. For each cluster, two reaction paths were studied with N2 initially on M and Fe atoms, respectively. Mayer bond order analysis provides more information on the activation of N−N bonds. M2Fe is generally more reactive than MFe2. The coordination mode of N2 on three metal atoms can be end‐on: end‐on: side‐on (EES) for both MFe2 and M2Fe. In addition, a unique end‐on: side‐on: side‐on (ESS) coordination mode was found for M2Fe, which leads to a higher degree of N−N bond activation. Nb2Fe has the highest reactivity towards N2 when both the transfer of N2 and the dissociation of N−N bonds are taken into account, while Ta‐containing clusters have a superior ability to activate the N−N bond. These results indicate that it is possible to improve the performance of iron‐based catalysts by doping with vanadium group metals.
Observation of a Long‐lived Electronic Coherence Modulated by Vibrational Dynamics in Molecular Nd3+‐Complexes at Room Temperature
Authors: Ghosh Jayanta , Gheibi Mirali , Kalas Tillmann , Sarpe Cristian , Zielinski Bastian , Ciobotea Ramela , Burghard Morscher Christoph , Koehne Ingo , Pietschnig Rudolf , Senftleben Arne , Baumert Thomas , Braun Hendrike 
Published: March 2023

AbstractTemporally delayed, phase‐locked coherent pairs of near IR femtosecond laser pulses were employed to study electronic coherences in molecular Nd3+‐complexes at room temperature. Dissolved and solid complexes were studied under a confocal microscope set‐up with fluorescence detection. The observed electronic coherence on a few hundred femtoseconds time scale is modulated by additional coherent wave packet dynamics, which we attribute mainly to be vibrational in nature. In future, the complexes may serve as prototypes for possible applications in quantum information technology.
Comparative analysis of infection process in Upland cottons differing in resistance to Fusarium wilt caused byFusarium oxysporumf. sp.vasinfectumrace 4
Authors: Zhu Yi , Abdelraheem Abdelraheem , Cooke Peter , Wheeler Terry , Dever Jane K. , Hake Kater , Bissonnette Kaitlyn , Zhang Jinfa 
Published: March 2023

AbstractFusarium wilt (Fusarium oxysporumf. sp.vasinfectumrace 4, FOV4) in cotton is an early season soil‐borne vascular disease including seedling wilt and death. Its infection process in Upland cotton is currently unknown. This study compared the infection process among three Upland cotton cultivars (Gossypium hirsutum) using a confocal microscope. Seedlings were grown in a hydroponic system to the two‐true leaf stage and inoculated with a virulent local FOV4 isolate. Based on the infection process, the resistant cultivar FM 2334GLT was divided into resistant (R) and susceptible (S) groups. The susceptible PHY 725 RF and FM 2334GLT(S) had the highest numbers of conidia attached and germinated on the root surface, followed by susceptible Acala 1517‐08. FM 2334GLT(R) had the lowest numbers. Hyphae penetration and colonization inside the root followed a similar trend. In PHY 725 RF, FM 2334GLT(S), and Acala 1517‐08, hyphae started to penetrate into epidermal cells at 2 days post inoculation (dpi), spread in the cortex intercellularly and intracellularly at 3–5 dpi, and entered the xylem vessels at 7 dpi, followed by colonization at 11–15 dpi. FM 2334GLT(R) had the lowest numbers of penetrating hyphae inside the root, with no apparent hyphae observed in the xylem. This study demonstrated that FM 2334GLT(R) had delayed infection, reduced fungal growth and reproduction, and prevented the FOV4 fungus from invading the xylem. The resistance in FM 2334GLT(R) was similar to that in Pima PHY 841 RF but with fewer hyphae in the cortex, suggesting possible different resistant mechanisms.
A CRISPR/Cas9‐engineered mouse carrying a conditional knockout allele for the early growth response‐1 transcription factor
Authors: Maurya Vineet K. , Ying Yan , Lanza Denise G. , Heaney Jason D. , Lydon John P. 
Published: March 2023

SummaryEarly growth response 1 (EGR1) mediates transcriptional programs that are indispensable for cell division, differentiation, and apoptosis in numerous physiologies and pathophysiologies. Whole‐body EGR1 knockouts in mice (Egr1KO) have advanced our understanding of EGR1 function in an in vivo context. To extend the utility of the mouse to investigate EGR1 responses in a tissue‐ and/or cell‐type‐specific manner, we generated a mouse model in which exon 2 of the mouse Egr1 gene is floxed by CRISPR/Cas9 engineering. The floxed Egr1 alleles (Egr1f/f) are designed to enable spatiotemporal control of Cre‐mediated EGR1 ablation in the mouse. To confirm that the Egr1f/f alleles can be abrogated using a Cre driver, we crossed the Egr1f/f mouse with a global Cre driver to generate the Egr1 conditional knockout (Egr1d/d) mouse in which EGR1 expression is ablated in all tissues. Genetic and protein analysis confirmed the absence of exon 2 and loss of EGR1 expression in the Egr1d/d mouse, respectively. Moreover, the Egr1d/d female exhibits overt reproductive phenotypes previously reported for the Egr1KO mouse. Therefore, studies described in this short technical report underscore the potential utility of the murine Egr1 floxed allele to further resolve EGR1 function at a tissue‐ and/or cell‐type‐specific level.