bluuebunny/crossref_metadata_2025_split huggingface dataset:
SNP2-Lys-C markers inheritance, genes expression, and the resistance of second-generation Catfish Clarias gariepinus against Aeromonas hydrophila infection
Authors: Hanggara Yudha , Alimuddin Alimuddin , Tri Soelistyowati Dinar Tri Soelistyowati , Nuryati Sri 
Published: January 2025

High mortality rates in catfish seed caused by motile Aeromonas septicemia disease caused by Aeromonas hydrophila infection. The primary focus is on the genetic selection of the second generation (G2) catfish for resistance to A. hydrophila, utilizing the type-C lysozyme gene at SNP-2 genotype TT. The objective is to assess the inheritance of the SNP2-Lys-C genetic marker from the first generation (G1) Sangkuriang breeders and to evaluate the resistance of the G2 seed to infection. In the experiment, G1 breeders with the TT genotype were spawned, and the resulting G2 seed were challenged with A. hydrophila. The findings indicate that all G2 seed possess the TT genotype and exhibit a higher survival rate compared to the dominant CT genotype seed. The expression of the Lys-C gene in G2 seed is elevated at the onset of infection, decreasing after the seventh day. The expression of the MHC1a and IL-1b genes is also associated with a higher survival rate. The survival of G2 seed correlates with the white blood cell and lysozyme activity, along with an increase in phagocytic activity peaking on the seventh day post-challenge. The conclusion of this study suggests that G2 catfish seed with the TT genotype have stronger resistance to A. hydrophila infection compared to seed with the dominant CT genotype.
Keywords: Aeromonas hydrophila, catfish, genotype, lysozyme, resistance

ABSTRAK

Mortalitas tinggi yang terjadi pada benih lele yang disebabkan oleh penyakit motile aeromonas septicemia akibat Aeromonas hydrophila. Fokus utama adalah pada seleksi genetik generasi kedua (G2) lele untuk resistensi terhadap A. hydrophila, menggunakan gen lisozim tipe-C pada SNP-2 genotipe TT. Studi ini bertujuan menilai pewarisan marka genetik SNP2-Lys-C dari induk sangkuriang generasi pertama (G1) dan mengevaluasi ketahanan benih G2 terhadap infeksi. Dalam eksperimen, induk G1 genotipe TT dipijahkan dan benih G2 yang dihasilkan diuji dengan bakteri A. hydrophila. Hasil menunjukkan bahwa semua benih G2 memiliki genotipe TT dan menunjukkan tingkat kelangsungan hidup yang lebih baik dibandingkan dengan benih dominan genotipe CT. Ekspresi gen Lys-C pada benih G2 tinggi pada awal infeksi, menurun setelah hari ketujuh. Ekspresi gen MHC1a dan IL-1b juga terkait dengan tingkat kelangsungan hidup yang lebih tinggi. Kelangsungan hidup benih G2 berkorelasi dengan jumlah sel darah putih dan aktivitas lisozim, serta peningkatan aktivitas fagositik yang mencapai puncak pada hari ketujuh. Kesimpulan studi ini menunjukkan bahwa benih lele G2 dengan genotipe TT memiliki resistensi yang lebih kuat terhadap infeksi A. hydrophila dibandingkan dengan benih dominan genotipe CT.
Kata Kunci: Aeromonas hydrophila, genotipe, ikan lele, lisozim, resistan
PELATIHAN PEMBUATAN DESSERT ASAL EROPA BARAT DI KAMPUNG GERENDENG PULO
Authors: Indrawan Nity , Burianto Etlivia , Jusan Putri Cicilia 
Published: January 2025

PKM atau Pengabdian Kepada Masyarakat adalah seseorang, kelompok, atau lembaga berusaha untuk memberikan kontribusi positif kepada masyarakat. Pengabdian Kepada Masyarakat seringkali terkait dengan pendidikan tinggi dan penelitian, di mana siswa dan akademisi terlibat dalam kegiatan yang dapat menggunakan pengetahuan dan keterampilan mereka untuk membantu memecahkan masalah, meningkatkan kesejahteraan, dan memberikan manfaat kepada masyarakat secara keseluruhan. Warga Kampung Gerendeng Pulo memiliki ketertarikan terhadap ide masak makanan manis yang dapat dibuat di rumah maka dilaksanakan PKM "Pelatihan Pembuatan Dessert Asal Eropa Barat” untuk memberikan pelatihan tentang cara membuat hidangan manis asal Eropa Barat. Terdiri dari dua hidangan manis asal Eropa barat yaitu PralinÃ© dan Rote GrÃ¼tze, hidangan manis yang dipilih disesuaikan dengan peralatan yang tersedia di tempat pelatihan. Pelatihan ini dilaksanakan dengan tujuan untuk meningkatkan ekonomi dan memenuhi permasalahan, yakni mencari ide masak hidangan manis dirumah dan ide bisnis masakan manis dengan biaya bahan yang terjangkau. Harapan yang ingin dicapai adalah agar warga Kampung Gerendeng Pulo dapat menggunakan produk dan layanan yang telah dipelajari, agar dapat memberikan manfaat dan meningkatkan pendapatan warga Kampung Gerendeng Pulo melalui usaha bisnis atau dagang.
Efficacy of Platelet-Rich Plasma Versus Placebo for the Treatment of Greater Trochanteric Pain Syndrome
Authors: Atchia Ismael , Ali Mohammed , Oderuth Eshan , Holleyman Richard , Malviya Ajay 
Published: January 2025

Background:
Greater trochanteric pain syndrome (GTPS) is a painful condition that can impair a patient’s quality of life. If nonoperative measures fail, progressively more invasive treatment options may be required. This clinical trial aimed to evaluate the effectiveness of ultrasound-guided leukocyte-rich platelet-rich plasma (LR-PRP) injections in the treatment of refractory GTPS caused by bursitis and/or gluteal tendinopathy.

Methods:
An ethically approved, adequately powered, double-blinded randomized controlled trial (RCT) was conducted to evaluate the clinical outcomes in randomized LR-PRP and placebo groups using the International Hip Outcome Tool-12 (iHOT-12), a visual analogue scale (VAS) for pain, the modified Harris hip score (mHHS), the EuroQol 5-Dimensions (EQ-5D) questionnaire, and the presence or absence of complications. All injections were performed under ultrasound guidance into the trochanteric bursa and gluteus medius tendon.

Results:
The final analysis included 79 patients (39 in the LR-PRP and 40 in the placebo group; 73 female and 6 male; all Caucasian). Both the LR-PRP and the placebo group generally had improvement from baseline that was maintained to 12 months. The only deterioration in scores compared to baseline was seen in the LR-PRP group for the iHOT-12 at 12 months, the EQ-5D index at 3 and 6 months, and the EQ-5D VAS at all follow-up time points. However, there was no significant difference between the 2 groups at any follow-up point (p > 0.05). A multivariable linear regression model, with adjustment for age, sex, body mass index, and preoperative baseline score, did not reveal any significant associations between iHOT-12 and EQ-5D score gains at 12 months and treatment.

Conclusions:
This randomized trial found no significant difference in outcomes between LR-PRP and placebo for the treatment of greater trochanteric pain up to 6 months following the intervention. As a result, we do not support the routine use of PRP for the treatment of this condition.

Level of Evidence:
Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
Teachers' experiences of a differentiated curriculum for children with autism spectrum disorder
Authors: Ameen Nausheen 
Published: January 2025

This study explored teachers' experiences in implementing a differentiated curriculum for children with severe intellectual disorders, including learners with autism spectrum disorder. The South African Differentiated Curriculum and Assessment Policy Statement (DCAPS) (2018) is a pilot study implemented by the Department of Basic Education (DBE) to adapt the existing Curriculum and Assessment Policy Statement (CAPS) to accommodate learners with high support needs to acquire skills and independence. This research followed a qualitative approach with a phenomenological multiple case study design. Eight teachers from four different schools in the Gauteng province participated. Data were collected through semi-structured interviews, document analysis, observations and field notes. In the interviews, teachers shared their thoughts, feelings and experiences about using the DCAPS (2018). The findings revealed the following major themes: (1) The teachers did not entirely understand the rationale behind the DCAPS (2018) curriculum for children with autism (2) teachers found the implementation of the differentiated curriculum difficult (3) teachers lacked sufficient training on the DCAPS (2018) curriculum. It was recommended that the Department of Basic Education (DBE) uses a professional developmental model that includes continuous monitoring and support. The study provides a stepping-stone for further research on the DCAPS (2018) curriculum for children with autism in South Africa.
From Downloads to Dollars: The Dominance of Active Users Over Downloads
Authors: Mukhi Sushree Sangeeta , Tiwari Bhupendra Bahadur , Kuriakose Annu 
Published: January 2025

Abstract

The research paper revolves around the business of health apps across the world and possible impact of the number of users and downloads on the revenue generation of health apps. With the help of trend analysis and linear regression methods, significant impact was studied on the variables like users, downloads and revenue of health apps for past 8 years (N=8) (2016 to 2023). The downloads (independent variable) didn’t show any significant impact on revenue (dependent variable) where p>0.05 whereas the users (independent variable) showed a significant impact on revenue (dependent variable) where the p<0.05. The users of these health apps play a significant role on the business of these health apps by directing impacting the revenue generation, whereas the downloads of these health apps don’t show any impact on the revenue generation of these health apps. The rise in the number of users boosted drastically during the pandemic year 2019 and 2020 and same impact can be seen on the revenue also.
Soil-moisture-atmosphere coupling hotspots and their representation in seasonal forecasts of boreal summer
Authors: Day Jonathan , Vitart Frederic , Stockdale Tim , de Rosnay Patricia , Ardilouze Constantin , Peano Daniele , Sanna Antonella , Fröhlich Kristina , Andrews Martin 
Published: January 2025

Abstract

This paper examines soil-moisture-atmosphere coupling "hotspots" in the Northern Hemisphere during the summer months and evaluates their representation in seasonal forecasts. Using hindcasts from the Copernicus Climate Change Service (C3S) multi-model seasonal forecast system the study explores the predictability of land-atmosphere interactions based on soil moisture anomalies. The results show that regions with strong soil-moisture-atmosphere coupling exhibit considerable potential for predicting seasonal temperature and precipitation patterns a season or more in advance. However, significant uncertainty exists in estimates of the soil-moisture initial conditions and soil-moisture persistence timescales. While some regions show realistic coupling, others, including the Central USA and Eastern Europe, display exaggerated coupling, leading to errors in temperature and precipitation forecasts. This study underscores the potential for predicting the atmosphere in summer based on memory of soil-moisture initial conditions whilst highlighting areas for further forecast system improvement.
Axl inhibition on dendritic cells enhances STING anticancer therapy through type I interferon signaling
Authors: Aguilera Todd , Gonzalez Isaac , Elghonaimy Eslam , Zhang Qiongwen , Montgomery Isaac , Leung Peter , Rodriguez Arely , Diegeler Sebastian , Swancutt Katy , Brekken Rolf 
Published: January 2025

Abstract

The Axl receptor tyrosine kinase is pivotal for metastatic tumor progression, tumor immune evasion, and regulating inflammation of innate immune cells. In this study we investigated Axl’s immune function in immunogenic tumors and found that Axl knockout (KO) mice exhibited a significant delay in tumor growth. Single-cell RNA sequencing revealed that Axl deficiency increases CD8 T cell activity. Tumor growth delay was dependent on CD8 T cells and BATF3 expression, indicating a role for Axl in regulating dendritic cell (DC) cross priming activities. Cre-driven conditional KO models further demonstrated that loss of Axl on DCs—but not on macrophages—was sufficient to slow tumor growth, a process reliant on type I interferon (IFN) signaling. Given Axl’s role in modulating IFN-I signaling, we discovered that its absence enhanced the effectiveness of STING agonists and improved the cross-priming capacity of both cDC1 and cDC2 subsets.
Investigation of the Utility of Global Leadership Initiative on Malnutrition Criteria as Prognostic Factors in Patients Undergoing Adjuvant S-1 Chemotherapy for Biliary Tract Cancer
Authors: Konaka Ryunosuke , Yanagimoto Hiroaki , Tsugawa Daisuke , Akita Masayuki , Mizumoto Takuya , Yoshida Toshihiko , Sou Shinichi , Ishida Jun , Nanno Yoshihide , Urade Takeshi , Fukushima Kenji , Gon Hidetoshi , Komatsu Shohei , Kido Masahiro , Toyama Hirochika , Fukumoto Takumi 
Published: January 2025

Abstract

Background
Biliary tract cancers (BTCs) have poor prognoses, with limited curative options beyond surgical resection. Adjuvant S-1 chemotherapy has shown survival benefits in Japanese patients with resected BTCs. However, prognostic factors influencing survival in these patients remain uncertain. In this study, we aimed to investigate the efficacy of preoperative nutritional status using Global Leadership Initiative on Malnutrition (GLIM) criteria as a prognostic factor in patients receiving adjuvant S-1 chemotherapy for BTCs.
Methods
In this retrospective study, we evaluated 58 patients who underwent curative surgery for BTCs, excluding intrahepatic cholangiocarcinoma, at Kobe University from 2013 to 2022, followed by adjuvant S-1 chemotherapy. Nutritional status was classified by GLIM criteria into normal/moderate and severe malnutrition groups. Overall survival (OS) and recurrence-free survival (RFS) were analyzed using Kaplan–Meier and Cox proportional hazards models.
Results
Of the 58 patients, 3.4% had no malnutrition, 72.5% had moderate malnutrition, and 24.1% had severe malnutrition. Patients with severe malnutrition had significantly worse OS (24.7% vs 52.5%, p = 0.0014) and RFS (34.3% vs 52.0%, p = 0.0066). Severe malnutrition was an independent prognostic factor for both OS (hazard ratio [HR]: 3.40; 95% confidence interval [CI]: 1.46–7.94, p = 0.0047) and RFS (HR: 2.48; 95% CI: 1.07–5.76, p = 0.035). No significant difference in S-1 completion rates was observed.
Conclusions
Severe malnutrition, as defined by GLIM criteria, is a poor prognostic factor in patients with BTCs undergoing adjuvant S-1 chemotherapy.
The Histone Demethylase LSD1/ZNF217/CoREST Complex is a Major Restriction Factor of Epstein-Barr Virus Lytic Reactivation
Authors: Gewurz Ben , Liao Yifei , Yan Jinjie , Kong Isabella , Li Zhixuan , Ding Weiyue , Clark Sarah , Guilino-Roth Lisa 
Published: January 2025

Abstract

Epstein-Barr virus (EBV) contributes to ~1.5% of human cancers, including lymphomas, gastric and nasopharyngeal carcinomas. In most of these, nearly 80 viral lytic genes are silenced by incompletely understood epigenetic mechanisms, precluding use of antiviral agents such as ganciclovir to treat the 200,000 EBV-associated cancers/year. To identify host factors critical for EBV latency, we performed a human genome-wide CRISPR-Cas9 screen in Burkitt B-cells. Top hits included the lysine-specific histone demethylase LSD1 and its co-repressors ZNF217 and CoREST. LSD1 removes histone 3 lysine 4 (H3K4) and histone 3 lysine 9 (H3K9) methylation marks to downmodulate chromatin activation. LSD1, ZNF217 or CoREST knockout triggered EBV reactivation, as did a LSD1 small molecule antagonist, whose effects were additive with histone deacetylase inhibition. LSD1 blockade reactivated EBV in Burkitt lymphoma, gastric carcinoma and nasopharyngeal carcinoma models, sensitized cells to ganciclovir cytotoxicity and induced EBV reactivation in murine xenografts. ZNF217 and LSD1 co-occupied the EBV immediate early gene BZLF1 promoter, which drives B-cell lytic cycle, as well as to the oriLyt enhancer regions recently implicated in EBV reactivation. LSD1 depletion increased levels of activating histone 3 lysine 4 (H3K4) methylation but not repressive histone lysine 9 methylation marks at BZLF1 and oriLyt and induced their interaction by long-range DNA looping. An orthogonal CRISPR screen highlighted a key H3K4 methyltransferase KMT2D role in driving EBV reactivation. Our results highlight H3K4 methylation as a major EBV lytic switch regulator and suggest novel therapeutic approaches.
Elevated polyglutamylation and tau phosphorylation levels in patients with primary central nervous system lymphoma are related to cognitive decline and neuropsychiatric changes at disease onset
Authors: Takeshima Yuki , Shinojima Naoki , Fujimoto Kenji , Yoshii Daiki , Hayakata Yasushi , Oya Masafumi , Tasaki Masayoshi , Mikami Yoshiki , Uetani Hiroyuki , Hirai Toshinori , Mukasa Akitake 
Published: January 2025

Abstract

Primary central nervous system lymphoma (PCNSL) often presents with nonspecific cognitive decline and neuropsychiatric changes at onset, yet the underlying mechanisms remain poorly understood. Polyglutamylation, a posttranslational modification, is associated with better responses to methotrexate-based chemotherapy in patients with PCNSL. On the other hand, hyperglutamylation of microtubules in neurons has been implicated in neurodegeneration via the accumulation of phosphorylated tau. This study aimed to elucidate the relationship of polyglutamylation with cognitive decline and neuropsychiatric changes at PCNSL onset. We retrospectively analyzed 145 patients with PCNSL treated at our institution between 2001 and 2022. Polyglutamylation and phosphorylated tau were evaluated using immunohistochemistry. In vitro studies were performed to evaluate the causal relationship between polyglutamylation and tau phosphorylation in PCNSL cell lines. Cognitive decline and neuropsychiatric changes at disease onset were observed in 48.3% of patients, with multivariate analysis identifying high polyglutamylation levels as an independent risk factor (odds ratio: 7.34, p < 0.001). Patient samples and cell lines consistently demonstrated a link between polyglutamylation and tau phosphorylation, implicating that polyglutamylation-induced tau phosphorylation may contribute to cognitive decline and neuropsychiatric changes. Our findings suggest that polyglutamylation contributes to neurocognitive dysfunction in PCNSL, and these results elucidate a novel aspect of PCNSL pathophysiology.
Explaining Twitter’s inability to reduce vaccine misinformation during the COVID-19 pandemic
Authors: Broniatowski David A. , Zhong Wei , Simons Joseph R. , Dredze Mark , Abroms Lorien C. 
Published: January 2025

Abstract

Users dissatisfied with exposure to objectionable online content have begun to migrate en masse to new social media platforms. These new platforms share architectural features with legacy platforms, but offer content moderation services that legacy platforms no longer employ at scale. Such migrations assume that moderation interventions, such as deplatforming and content removal, are efficacious; however, this claim has not been evaluated based on evidence. We therefore evaluated the efficacy of Twitter’s attempts to curtail vaccine misinformation during the COVID-19 pandemic. We found that vaccine skeptical accounts generated a larger share of tweets about vaccines, increased in virality, and became more misinformative after Twitter began removing content and accounts. We also found evidence that Twitter’s mass deplatforming of 70,000 accounts on January 8, 2021 preceded an increase in misinformation, calling into question the efficacy of these removals. Novel platforms that share Twitter’s architecture may therefore face similar moderation challenges.
Research on the Expression and Mechanism of LRRC8D in Esophageal Squamous Cell Carcinoma
Authors: Mo Guanyong , Yan Jingwei , Zhou Huafu , Luo Yichen , Song Wentao , Lou Chenxi , Tan Linyuan , Zheng Jinmin , Li Baijun , Chen Shangwei 
Published: January 2025

Abstract

Purpose
The leucine-rich repeat-containing 8 (LRRC8) protein family, particularly leucine-rich repeat containing 8 family, member D (LRRC8D), has been implicated in various cancers. However, its role in esophageal squamous cell carcinoma (ESCC) remains unclear. This study aims to evaluate LRRC8D as a potential diagnostic biomarker for ESCC and to elucidate its regulatory mechanisms.
Materials and methods
The expression of LRRC8D in ESCC was analyzed using publicly available datasets, in-house RNA sequencing data, and immunohistochemistry. Associations between LRRC8D expression and clinical features were assessed using RStudio 4.3.2. Immune cell infiltration and its relationship with LRRC8D were investigated using the CIBERSORT R package. Kaplan-Meier survival analysis evaluated the prognostic relevance of LRRC8D. Functional enrichment analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, and protein-protein interaction (PPI) networks construction, were performed to identify core genes and pathways associated with LRRC8D.
Results
LRRC8D expression at both the mRNA and protein levels was significantly upregulated in ESCC tissues compared to non-cancerous tissues. While LRRC8D expression did not correlate with clinical characteristics such as age, gender, or tumor stage, it was significantly associated with immune cell composition in the tumor microenvironment. Survival analysis revealed no significant relationship between LRRC8D expression and patient outcomes, although higher expression levels showed a trend toward improved survival. Functional network analyses identified core genes associated with LRRC8D that play key roles in cell cycle regulation and mitosis. Enrichment analyses further highlighted LRRC8D's involvement in the cell cycle and related signaling pathways.
Conclusion
LRRC8D is a promising diagnostic biomarker for distinguishing ESCC tissues from non-cancerous tissues but lacks significant prognostic value. Its expression is closely associated with immune cell dynamics in ESCC and may influence tumor phenotypes through cell cycle regulation.
Do patients with a unilateral peripheral vestibular disorder need psychological support?
Authors: Lijić Laura , Mendeš Tihana , Včeva Andrijana , Zubčić Željko , Mihalj Hrvoje , Rezo Matej , Kralik Kristina , Vidić Hrvoje , Kopf Tamara , Gudelj Antoneta , Russo Andrea , Šoda Joško , Kljajic Zlatko 
Published: January 2025

Abstract

Nowadays, vestibular diseases are quite common in the population and significantly impair quality of life. Additionally, a notable problem is that a certain number of patients experience psychological consequences. Therefore, it is important to investigate the connection between vestibular damage and the potential need for psychological intervention. This study aims to examine the correlation between anxiety in individuals with unilateral peripheral vestibular disorder in relation to gender, age, and the existence of acute and chronic, compensated and uncompensated, partial and complete unilateral peripheral vestibular disorder. Additionally, it was important to determine the degree of free-floating anxiety, phobia-related anxiety, obsessiveness, somatic manifestations, depression, and hysteria in these patients using the Crown-Crisp Experience Index questionnaire. The study included 54 patients, 39 (72%) female and 15 (28%) male, with an average age of 61.5 ± 13.5 years. Results showed that the majority of participants had pronounced somatic anxiety (29.6%), followed by depression (14.8%), free-floating anxiety (9.4%), phobia-related anxiety (7.4%) and hysteria (7.4%) equally represented, and obsessiveness (1.8%) being the least pronounced. Nearly all participants (94%) were diagnosed with chronic hypofunction due to presenting for examination and diagnostic processing after the acute phase of the disease had ended. To conclude, no significant differences were observed in the common symptoms or personality traits within conventional categories of psychoneurotic and personality disorders concerning gender or the presence of vestibular hypofunction.