bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fatigue life prediction of stud shear connectors under corrosion-fatigue coupling effect
Authors: Xiao Lin , Huang Yaxi , Wei Xing 
Published: March 2023

AbstractBased on the three-stage fatigue crack growth model, a corrosion fatigue life prediction method considering the coupling effect of corrosion and fatigue is proposed in this paper. In this case, stress factor amplitude was claimed considering the coupling effect of corrosion and fatigue. Three push-out tests in corrosion conditions were conducted to study the failure mode of studs. The crack propagation of studs, obtained through the push-out tests, was simulated in FRANC 3D to establish a library of adequate stress factor amplitude. According to the corrosion degree of the specimens, the corrosion dissolution rate formula was formed, and the corrosion fatigue life of the specimen was predicted. Results show that the error between the predicted and experimental values is approximately 25%.
Involvement of 2′-5′ oligoadenylate synthetase-like protein in the survival of Mycobacterium tuberculosis avirulent strain in macrophages
Authors: Reheman Aikebaier , Cao Xiaojian , Wang Yifan , Nie Xi , Cao Gang , Zhou Wei , Yang Bing , Lei Yingying , Zhang Weipan , Naeem Muhammad Ahsan , Chen Xi 
Published: March 2023

AbstractMycobacterium tuberculosis (M. tuberculosis) can replicate in the macrophage by interfering with many host protein functions. While it is far from known these host proteins for controlling M. tuberculosis infection. Herein, we infected macrophages including THP-1 and Raw264.7 cells with M. tuberculosis and identified the differentially expressed genes (DEGs) in the interferon signaling pathway. Among them, 2′-5′ oligoadenylate synthetase-like (OASL) underwent the greatest upregulation in M. tuberculosis-infected macrophages. Knockdown of the expression of OASL attenuated M. tuberculosis survival in macrophages. Further, bioinformatics analysis revealed the potential interaction axis of OASL-TAB3- Rv0127, which was further validated by the yeast-two-hybrid (Y2H) assay and Co-IP. This interaction axis might regulate the M. tuberculosis survival and proliferation in macrophages. The study reveals a possible role of OASL during M. tuberculosis infection as a target to control its propagation.
“A Simple, Short, and Exact Account of the Facts”?
Authors: Starling Drew 
Published: March 2023

AbstractThis article examines how material constraints thought to stifle political news' production in France actually encouraged a small number of Jansenist priests, their lay defenders, and book-trade workers to develop new printing methods and a sophisticated print distribution system that enabled them to skirt policing in the production and distribution of the Nouvelles ecclésiastiques (1728–1803). The existence of the Nouvelles ecclésiastiques outside the official book trade liberated its authors to include content that no other paper could. The dense religious dispute about which it sought to inform readers, coupled with its uninitiated target audience, encouraged its authors not just to report the news but also to explain it in highly partisan terms. In so doing, the Nouvelles ecclésiastiques provided many readers with an initiation into news reading. While the news reported concerned a religious controversy, it worked to reshape how readers thought about religion and politics in early eighteenth-century France.
Meister and Jupille
Authors: Priest Robert D. 
Published: March 2023

AbstractIn 1885 Louis Pasteur successfully treated two boys from different parts of rural France, Joseph Meister and Jean-Baptiste Jupille, with his experimental rabies vaccine. Arguing that the boys played an important role in shaping images of Pasteur and his vaccine in French culture, this article reconstructs their long relationships with the scientist and then traces their evolving cultural representations during the Third Republic up to 1940. Meister, a young child from Alsace who sought salvation in Paris, was particularly assimilable to nationalist narratives that Pasteur himself encouraged. Jupille, in fighting with a rabid dog to save young children from attack, could provide an exemplar of the selfless yet virile male adolescent whom late nineteenth-century authorities sought to produce. Both boys' stories produced associations that reflected favorably on Pasteur and the Pastorians, yet each also held an independent appeal at particular moments in modern French history.
Radiosurgical Corpus Callosotomy for Intractable Epilepsy: Retrospective Long-Term Safety and Efficacy Assessment in 19 Patients an Review of the Literature
Authors: Hamdi Hussein , Boissonneau Sébastien , Valton Luc , McGonigal Aileen , Bartolomei Fabrice , Regis Jean 
Published: March 2023

BACKGROUND:Some patients suffering from intractable epileptic seizures, particularly drop attacks (DAs), are nonremediable by curative techniques. Palliative procedure carries a significant rate of surgical and neurological complications.OBJECTIVE:To propose evaluation of safety and efficacy of Gamma Knife corpus callosotomy (GK-CC) as an alternative to microsurgical corpus callosotomy.METHODS:This study included retrospective analysis of 19 patients who underwent GK-CC between 2005 and 2017.RESULTS:Of the 19 patients, 13 (68%) had improvement in seizure control and 6 had no significant improvement. Of the 13/19 (68%) with improvement in seizures, 3 (16%) became completely seizure-free, 2 (11%) became free of DA and generalized tonic-clonic but with residual other seizures, 3 (16%) became free of DA only, and 5 (26%) had >50% reduction in frequency of all seizure types. In the 6 (31%) patients with no appreciable improvement, there were residual untreated commissural fibers and incomplete callosotomy rather than failure of Gamma Knife to disconnect. Seven patients showed a transient mild complication (37% of patients, 33% of the procedures). No permanent complication or neurological consequence was observed during the clinical and radiological workup with a mean of 89 (42-181) months, except 1 patient who had no improvement of epilepsy and then aggravation of the pre-existing cognitive and walking difficulties (Lennox-Gastaut). The median time of improvement after GK-CC was 3 (1-6) months.CONCLUSION:Gamma Knife callosotomy is safe and accurate with comparable efficacy to open callosotomy in this cohort of patients with intractable epilepsy suffering from severe drop attacks.
A Comparison of Outcomes Between Transfemoral Versus Transradial Access for Carotid Stenting
Authors: El Naamani Kareem , Khanna Omaditya , Syal Amit , Momin Arbaz A. , Abbas Rawad , Amllay Abdelaziz , Sambangi Abhijeet , Hunt Adam , Dougherty Jaime , Lawall Charles L. , Tjoumakaris Stavropoula I , Gooch M. Reid , Herial Nabeel A , Rosenwasser Robert H , Zarzour Hekmat , Schmidt Richard F , Jabbour Pascal M 
Published: March 2023

BACKGROUND:
The transradial (TR) approach has emerged as an alternative to the transfemoral (TF) approach in carotid artery stenting (CAS) because of its perceived benefits in access site complications and overall patient experience.

OBJECTIVE:
To assess outcomes of TF vs TR approach for CAS.

METHODS:
This is a retrospective single-center review of patients receiving CAS through the TR or TF route between 2017 and 2022. All patients with symptomatic and asymptomatic carotid disease who underwent attempted CAS were included in our study.

RESULTS:
A total of 342 patients were included in this study: 232 underwent CAS through TF approach vs 110 through the TR route. On univariate analysis, the rate of overall complications was more than double for the TF vs TR cohort; however, this did not achieve statistical significance (6.5% vs 2.7%, odds ratio [OR] = 0.59 P = .36). The rate of cross-over from TR to TF was significantly higher on univariate analysis (14.6 % vs 2.6%, OR = 4.77, P = .005) and on inverse probability treatment weighting analysis (OR = 6.11, P < .001). The rate of in-stent stenosis (TR: 3.6% vs TF: 2.2%, OR = 1.71, P = .43) and strokes at follow-up (TF: 2.2% vs TR: 1.8%, OR = 0.84, P = .84) was not significantly different. Finally, median length of stay was comparable between both cohorts.

CONCLUSION:
The TR approach is safe, feasible, and provides similar rates of complications and high rates of successful stent deployment compared with the TF route. Neurointerventionalists adopting the radial first approach should carefully assess the preprocedural computed tomography angiography to identify patients amenable to TR approach for carotid stenting.
Dynein at the kinetochore
Authors: Gassmann Reto 
Published: March 2023

ABSTRACT
The microtubule minus-end-directed motility of cytoplasmic dynein 1 (dynein), arguably the most complex and versatile cytoskeletal motor, is harnessed for diverse functions, such as long-range organelle transport in neuronal axons and spindle assembly in dividing cells. The versatility of dynein raises a number of intriguing questions, including how is dynein recruited to its diverse cargo, how is recruitment coupled to activation of the motor, how is motility regulated to meet different requirements for force production and how does dynein coordinate its activity with that of other microtubule-associated proteins (MAPs) present on the same cargo. Here, these questions will be discussed in the context of dynein at the kinetochore, the supramolecular protein structure that connects segregating chromosomes to spindle microtubules in dividing cells. As the first kinetochore-localized MAP described, dynein has intrigued cell biologists for more than three decades. The first part of this Review summarizes current knowledge about how kinetochore dynein contributes to efficient and accurate spindle assembly, and the second part describes the underlying molecular mechanisms and highlights emerging commonalities with dynein regulation at other subcellular sites.
Characterization of the nuclear import of the human CHD4–NuRD complex
Authors: Hoffmeister Helen , Holzinger Simon , Dürr Marie-Sofie , Bruckmann Astrid , Schindler Susanne , Gröbner-Ferreira Regina , Depping Reinhard , Längst Gernot 
Published: March 2023

ABSTRACT
Chromatin remodeling enzymes form large multiprotein complexes that play central roles in regulating access to the genome. Here, we characterize the nuclear import of the human CHD4 protein. We show that CHD4 enters the nucleus by means of several importin-α proteins (1, 5, 6 and 7), but independently of importin β1. Importin α1 directly interacts with a monopartite ‘KRKR’-motif in the N-terminus of CHD4 (amino acids 304–307). However, alanine mutagenesis of this motif only leads to an ∼50% reduction in nuclear localization of CHD4, implying that there are additional import mechanisms. Interestingly, we could show that CHD4 was already associated with the nucleosome remodeling deacetylase (NuRD) core subunits, such as MTA2, HDAC1 and RbAp46 (also known as RBBP7), in the cytoplasm, suggesting an assembly of the NuRD core complex before nuclear import. We propose that, in addition to the importin-α-dependent nuclear localization signal, CHD4 is dragged into the nucleus by a ‘piggyback’ mechanism using the import signals of the associated NuRD subunits.
Endosomal sorting sorted – motors, adaptors and lessons fromin vitroand cellular studies
Authors: Redpath Gregory M. I , Ananthanarayanan Vaishnavi 
Published: March 2023

ABSTRACTMotor proteins are key players in exerting spatiotemporal control over the intracellular location of membrane-bound compartments, including endosomes containing cargo. In this Review, we focus on how motors and their cargo adaptors regulate positioning of cargoes from the earliest stages of endocytosis and through the two main intracellular itineraries: (1) degradation at the lysosome or (2) recycling back to the plasma membrane. In vitro and cellular (in vivo) studies on cargo transport thus far have typically focussed independently on either the motor proteins and adaptors, or membrane trafficking. Here, we will discuss recent studies to highlight what is known about the regulation of endosomal vesicle positioning and transport by motors and cargo adaptors. We also emphasise that in vitro and cellular studies are often performed at different scales, from single molecules to whole organelles, with the aim to provide a perspective on the unified principles of motor-driven cargo trafficking in living cells that can be learned from these differing scales.
The potential of myosin and actin in nanobiotechnology
Authors: Månsson Alf 
Published: March 2023

ABSTRACT
Since the late 1990s, efforts have been made to utilize cytoskeletal filaments, propelled by molecular motors, for nanobiotechnological applications, for example, in biosensing and parallel computation. This work has led to in-depth insights into the advantages and challenges of such motor-based systems, and has yielded small-scale, proof-of-principle applications but, to date, no commercially viable devices. Additionally, these studies have also elucidated fundamental motor and filament properties, as well as providing other insights obtained from biophysical assays in which molecular motors and other proteins are immobilized on artificial surfaces. In this Perspective, I discuss the progress towards practically viable applications achieved so far using the myosin II–actin motor–filament system. I also highlight several fundamental pieces of insights derived from the studies. Finally, I consider what may be required to achieve real devices in the future or at least to allow future studies with a satisfactory cost–benefit ratio.
Contrasting effects of fungicide and herbicide active ingredients and their formulations on bumblebee learning and behaviour
Authors: Thompson Linzi J. , Stout Jane C. , Stanley Dara A. 
Published: March 2023

ABSTRACT
Fungicides and herbicides are two of the most heavily applied pesticide classes in the world, but receive little research attention with regards to their potential impacts on bees. As they are not designed to target insects, the mechanisms behind potential impacts of these pesticides are unclear. It is therefore important to understand their influence at a range of levels, including sublethal impacts on behaviours such as learning. We used the proboscis extension reflex (PER) paradigm to assess how the herbicide glyphosate and the fungicide prothioconazole affect bumblebee olfactory learning. We also assessed responsiveness, and compared the impacts of these active ingredients and their respective commercial formulations (Roundup Biactive and Proline). We found that learning was not impaired by either formulation but, of the bees that displayed evidence of learning, exposure to prothioconazole active ingredient increased learning level in some situations, while exposure to glyphosate active ingredient resulted in bumblebees being less likely to respond to antennal stimulation with sucrose. Our data suggest that fungicides and herbicides may not negatively impact olfactory learning ability when bumblebees are exposed orally to field-realistic doses in a lab setting, but that glyphosate has the potential to cause changes in responsiveness in bees. As we found impacts of active ingredients and not commercial formulations, this suggests that co-formulants may modify impacts of active ingredients in the products tested on olfactory learning without being toxic themselves. More research is needed to understand the mechanisms behind potential impacts of fungicides and herbicides on bees, and to evaluate the implications of behavioural changes caused by glyphosate and prothioconazole for bumblebee fitness.
Incubation temperature alters stripe formation and head colouration in American alligator hatchlings and is unaffected by estradiol-induced sex reversal
Authors: Rogerson Grace , Bock Samantha , Loera Yeraldi , Parrott Benjamin , Mulley John F. 
Published: March 2023

ABSTRACTConsiderations of the impact climate change has on reptiles are typically focused on habitat change or loss, range shifts and skewed sex ratios in species with temperature-dependent sex determination. Here, we show that incubation temperature alters stripe number and head colouration of hatchling American alligators (Alligator mississippiensis). Animals incubated at higher temperatures (33.5°C) had, on average, one more stripe than those at lower temperatures (29.5°C), and also had significantly lighter heads. These patterns were not affected by estradiol-induced sex reversal, suggesting independence from hatchling sex. Therefore, increases in nest temperatures as a result of climate change have the potential to alter pigmentation patterning, which may have implications for offspring fitness.
Plasma Exosome Gene Signature Differentiates Colon Cancer from Healthy Controls
Authors: Vallejos Paul A. , Gonda Amber , Yu Jingjing , Sullivan Brittany G. , Ostowari Arsha , Kwong Mei Li , Choi Audrey , Selleck Matthew J. , Kabagwira Janviere , Fuller Ryan N. , Gironda Daniel J. , Levine Edward A. , Hughes Christopher C. W. , Wall Nathan R. , Miller Lance D. , Senthil Maheswari 
Published: March 2023

Abstract
Background
Liquid biopsies have become an integral part of cancer management as minimally invasive options to detect molecular and genetic changes. However, current options show poor sensitivity in peritoneal carcinomatosis (PC). Novel exosome-based liquid biopsies may provide critical information on these challenging tumors. In this initial feasibility analysis, we identified an exosome gene signature of 445 genes (ExoSig445) from colon cancer patients, including those with PC, that is distinct from healthy controls.

Methods
Plasma exosomes from 42 patients with metastatic and non-metastatic colon cancer and 10 healthy controls were isolated and verified. RNAseq analysis of exosomal RNA was performed and differentially expressed genes (DEGs) were identified by the DESeq2 algorithm. The ability of RNA transcripts to discriminate control and cancer cases was assessed by principal component analysis (PCA) and Bayesian compound covariate predictor classification. An exosomal gene signature was compared with tumor expression profiles of The Cancer Genome Atlas.

Results
Unsupervised PCA using exosomal genes with greatest expression variance showed stark separation between controls and patient samples. Using separate training and test sets, gene classifiers were constructed capable of discriminating control and patient samples with 100% accuracy. Using a stringent statistical threshold, 445 DEGs fully delineated control from cancer samples. Furthermore, 58 of these exosomal DEGs were found to be overexpressed in colon tumors.

Conclusions
Plasma exosomal RNAs can robustly discriminate colon cancer patients, including patients with PC, from healthy controls. ExoSig445 can potentially be developed as a highly sensitive liquid biopsy test in colon cancer.
The open field assay is influenced by room temperature and by drugs that affect core body temperature
Authors: Jimenez Jessica A. , McCoy Eric S. , Lee David F. , Zylka Mark J. 
Published: March 2023

Background: The open field assay is used to study anxiety-related traits and anxiolytic drugs in rodents. This assay entails measuring locomotor activity and time spent in the center of a chamber that is maintained at ambient room temperature. However, the ambient temperature in most laboratories varies daily and seasonally and can differ between buildings. We sought to evaluate how varying ambient temperature and core body temperature (CBT) affected open field locomotor activity and center time of male wild-type (WT, C57BL/6) and Transient Receptor Potential Subfamily M Member 8 (Trpm8) knock-out (Trpm8-/-) mice. TRPM8 is an ion channel that detects cool temperatures and is activated by icilin. Methods: Mice were placed in the open field at 4°C and 23°C for 30 minutes. Distance traveled and time spent in the center were measured. Mice were injected with icilin, M8-B, diazepam, or saline, and changes in activity level were recorded. Results: The cooling agent icilin increased CBT and profoundly reduced distance traveled and center time of WT mice relative to controls. Likewise, cooling the ambient temperature to 4°C reduced distance traveled and center time of WT mice relative to Trpm8-/- mice. Conversely, the TRPM8 antagonist (M8-B) reduced CBT and increased distance traveled and center time of WT mice when tested at 4°C. The TRPM8 antagonist (M8-B) had no effect on CBT or open field behavior of Trpm8-/- mice. The anxiolytic diazepam reduced CBT in WT and Trpm8-/- mice. When tested at 4°C, diazepam increased distance traveled and center time in WT mice but did not alter open field behavior of Trpm8-/- mice. Conclusions: Environmental temperature and drugs that affect CBT can influence locomotor behavior and center time in the open field assay, highlighting temperature (ambient and core) as sources of environmental and physiologic variability in this commonly used behavioral assay.
Case Report: Actinomyces meyeri empyema in a COVID-19 positive patient with history of testicular mixed germ cell tumor
Authors: Behaeddin Bita Crystal , Barcena Hunter , Fonarov Ilya , Casadesus Damian 
Published: March 2023

The most common cause of empyema is a complicated parapneumonic effusion, but other foci of infection may also spread to the pleural space. A man in his early 30s with a history of testicular mixed germ cell tumor presented with a week of pleuritic chest pain. On admission, he was tachypneic, tachycardic, and had tested positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Imaging revealed a loculated large left pleural effusion with collapse of the left lung. A pigtail catheter was inserted, and the pleural effusion was evacuated. Fluid analysis indicated infection with Actinomyces meyeri. Clinical exam and mandible radiography ruled out infectious facial involvement. Intravenous ampicillin was started, but two days later the patient requested that the chest tube be removed, and he left the hospital against medical advice. The patient followed-up in our clinic one month later with a significantly improved medical condition. Treatment with oral amoxicillin-clavulanate for twelve months was prescribed.