bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of prognostic factors for vocal fold leukoplakia based on 344 cases at a two-year follow up
Authors: Yin S , Huang H , Sun P , Zhang D 
Published: May 2023

AbstractObjectiveTo evaluate risk factors for poor prognosis in vocal fold leukoplakia.MethodsClinical data were collected for 344 patients with vocal fold leukoplakia who received surgical treatment in our otolaryngology department from October 2010 to June 2019. Univariate and multivariate logistic regression analyses of the relevant factors were conducted.ResultsAmong the 344 patients, 98 exhibited recurrence and 30 underwent a malignant change. Multivariate logistic regression analysis showed that size of the lesion (p = 0.03, odds ratio = 2.14), form of the lesion under white light (p < 0.001), surgical method (p < 0.001, odds ratio = 0.28) and pathological type (p < 0.001) were independent factors that affected the recurrence of vocal fold leukoplakia. In both univariate and multivariate analyses, the sole independent risk factor for malignant transformation of vocal fold leukoplakia was pathological type (p < 0.001).ConclusionThe outlook for vocal fold leukoplakia depends on several clinical factors, especially pathological type. The more severe the pathological type, the more likely it is to recur or become cancerous.
Elders’ perspectives and priorities for ageing well in a remote Aboriginal community
Authors: McCausland Ruth , Jamieson Sacha Kendall , Robinson Virginia , Spencer Wendy , MacGillivray Peta , Andersen Melanie 
Published: May 2023

AbstractAboriginal Elders in Australia are recognised as having an important role as community leaders and cultural knowledge holders. However, the effects of colonisation and institutional racism mean Elders also experience significant social and economic disadvantage and poor health outcomes. There has been a systemic lack of attention to the worldviews and priorities of Aboriginal people as they age. In this article, we detail the findings of a qualitative study using a localised Aboriginal Elder-informed methodology that involved interviews and focus groups with 22 Aboriginal Elders in the remote town of Walgett on what ageing well means to them. This study was undertaken as part of a long-term partnership between a unique community-controlled Elders organisation and a university. The findings illuminate the barriers and enablers to ageing well for Aboriginal people in Walgett and elsewhere, and demonstrate the value to research, policy and service delivery of listening to and learning from Elders, centring Indigenous knowledges and worldviews, and bringing a more holistic conceptualisation of wellbeing to the understanding of what it means to age well.
On a class of nonlocal continuity equations on graphs
Authors: Esposito Antonio , Patacchini Francesco Saverio , Schlichting André 
Published: May 2023

AbstractMotivated by applications in data science, we study partial differential equations on graphs. By a classical fixed-point argument, we show existence and uniqueness of solutions to a class of nonlocal continuity equations on graphs. We consider general interpolation functions, which give rise to a variety of different dynamics, for example, the nonlocal interaction dynamics coming from a solution-dependent velocity field. Our analysis reveals structural differences with the more standard Euclidean space, as some analogous properties rely on the interpolation chosen.
Association between anti-Müllerian hormone levels and reproductive parameters in Wagyu cattle raised in Brazil
Authors: de Marchi Flávia , Lazzaretti Renan , de Camargo Janine , Facioli Fernanda Luiza , Zanella Eraldo Lourenso , Nacib Jorge-Neto Pedro , Groke Marques Mariana , Caires Kyle Cody , Zanella Ricardo 
Published: May 2023

SummaryThe production of in vitro embryos has sped up the dissemination of superior genetic material. However, the variation among the cattle response to oocyte and embryo production is a challenging factor. This variation is even higher in the Wagyu cattle as the breed has a small effective population size. The identification of an effective marker related to reproductive efficiency would allow the selection of more responsive females to reproductive protocols. The objective of this study was to evaluate the blood levels of anti-Müllerian hormone and associate it with oocyte recovery and blastocyst rate of embryos produced in vitro in Wagyu cows, as well as observe the hormone circulating levels in males. Serum samples from 29 females with seven follicular aspirations and four bulls were used. AMH measurements were performed using the bovine AMH ELISA kit. A positive correlation was identified between oocyte production and blastocyst rate (r = 0.84, P = 9 × 10−9), and AMH levels with oocyte (r = 0.49, P = 0.006) and embryo (r = 0.39, P = 0.03) production. The mean levels of AMH were different between animals with low (11.06 ± 3.01) and high (20.75 ± 4.46) oocyte production (P = 0.01). Males showed high serological levels of AMH (3829 ± 2328 pg/ml) compared with other breeds. It is possible to use the serological measurement of AMH as a method to select Wagyu females with greater capacity for oocyte and embryo production. Further studies correlating AMH serological levels with Sertoli cell function in bulls are needed.
Getting away with it? Kleptocracy, atrocities, and the morality of autocratic exile
Authors: Nili Shmuel 
Published: May 2023

AbstractForeign exile has often served as an important solution to high-stakes standoffs between opposition forces and beleaguered autocrats. I assess the moral status of autocratic exile, by focusing on the tension between exile's contribution to domestic peace and its threat to global deterrence against autocracy. I begin by contending that transitioning societies normally have the moral prerogative of accepting an exile arrangement for their autocrat, even though such an arrangement harms global deterrence against autocracy. I then suggest that, in the absence of clear evidence of majority opposition to an exile arrangement within the transitioning society, foreign countries who have been entangled in an autocrat's rule will normally have a decisive duty to facilitate his exile, despite exile's repercussions for global deterrence. I explain why such foreign entanglement, particularly on the part of affluent Western democracies, is inevitable in the case of kleptocrats. But I also show that the entanglement argument for exile extends even to murderous autocrats, whose crimes fall under the purview of the International Criminal Court. Countries entangled in a murderous autocrat's rule ought to prioritize their particular duties toward his victims over their general moral reasons to advance international criminal justice.
Structural Relationships Across the Sevier Gravity Slide of Southwest Utah and Implications for Catastrophic Translation and Emplacement Processes of Long Runout Landslides
Authors: Braunagel Michael J. , Griffith W. Ashley , Biek Robert F. , Hacker David B. , Rowley Peter D. , Malone David H. , Mayback Danika , Rivera Tiffany A. , Loffer Zachary , Smith Zachary D. 
Published: May 2023

AbstractThe physical processes that facilitate long‐distance translation of large‐volume gravity slides remain poorly understood. To better understand these processes and the controls on runout distance, we conducted an outcrop and microstructural characterization of the Sevier gravity slide across the former land surface and summarize findings of four key sites. The Sevier gravity slide is the oldest of three mega‐scale (>1,000 km2) collapse events of the Marysvale volcanic field (Utah, USA). Field observations of intense deformation, clastic dikes, pseudotachylyte, and consistency of kinematic indicators support the interpretation of rapid emplacement during a single event. Furthermore, clastic dikes and characteristics of the slip zone suggest emplacement involved mobilization and pressurized injection of basal material. Across the runout distance, we observe evidence for progressive slip delocalization along the slide base. This manifests as centimeter‐ to decimeter‐thick cataclastic basal zones and abundant clastic dikes in the north and tens of meters thick basal zones characterized by widespread deformation of both slide blocks and underlying rock near the southern distal end of the gravity slide. Superimposed on this transition are variations in basal zone characteristics and slide geometry arising from interactions between slide blocks during dynamic wear and deposition processes and pre‐existing topography of the former land surface. These observations are synthesized into a conceptual model in which the presence of highly pressurized fluids reduced the frictional resistance to sliding during the emplacement of the Sevier gravity slide, and basal zone evolution controlled the effectiveness of dynamic weakening mechanisms across the former land surface.
Toward a Better Understanding of Wildfire Behavior in the Wildland‐Urban Interface: A Case Study of the 2021 Marshall Fire
Authors: Juliano Timothy W. , Lareau Neil , Frediani Maria E. , Shamsaei Kasra , Eghdami Masih , Kosiba Karen , Wurman Joshua , DeCastro Amy , Kosović Branko , Ebrahimian Hamed 
Published: May 2023

AbstractOn 30 December 2021, the Marshall Fire devastated the Boulder, Colorado region. The fire initiated in fine fuels in open space just southeast of Boulder and spread rapidly due to the strong, downslope winds that penetrated into the Boulder Foothills. Despite the increasing occurrence of wildland‐urban interface (WUI) disasters, many questions remain about how fires progress through vegetation and the built environment. To help answer these questions for the Marshall Fire, we use a coupled fire‐atmosphere model and Doppler on Wheels (DOW) observations to study the fire's progression as well as examine the physical drivers of its spread. Evaluation of the model using the DOW suggests that the model is able to capture general characteristics of the flow field; however, it does not produce as robust of a hydraulic jump as the one observed. Our results highlight limitations of the model that should be addressed for successful WUI simulations.
Phase-tailored assembly and encoding of dissipative soliton molecules
Authors: Liu Yusong , Huang Siyun , Li Zilong , Liu Haoguang , Sun Yixiang , Xia Ran , Yan Lisong , Luo Yiyang , Liu Huanhuan , Xu Gang , Sun Qizhen , Tang Xiahui , Shum Perry Ping 
Published: May 2023

AbstractSelf-assembly of particle-like dissipative solitons, in the presence of mutual interactions, emphasizes the vibrant concept of soliton molecules in varieties of laser resonators. Controllable manipulation of the molecular patterns, held by the degrees of freedom of internal motions, still remains challenging to explore more efficient and subtle tailoring approaches for the increasing demands. Here, we report a new phase-tailored quaternary encoding format based on the controllable internal assembly of dissipative soliton molecules. Artificial manipulation of the energy exchange of soliton-molecular elements stimulates the deterministic harnessing of the assemblies of internal dynamics. Self-assembled soliton molecules are tailored into four phase-defined regimes, thus constituting the phase-tailored quaternary encoding format. Such phase-tailored streams are endowed with great robustness and are resistant to significant timing jitter. All these results experimentally demonstrate the programmable phase tailoring and exemplify the application of the phase-tailored quaternary encoding, prospectively promoting high-capacity all-optical storage.
An ultrahigh sensitivity acoustic sensor system for weak signal detection based on an ultrahigh-Q CaF2 resonator
Authors: Xing Tong , Xing Enbo , Jia Tao , Li Jianglong , Rong Jiamin , Li Li , Tian Sicong , Zhou Yanru , Liu Wenyao , Tang Jun , Liu Jun 
Published: May 2023

AbstractAcoustic sensors with ultrahigh sensitivity, broadband response, and high resolution are essential for high-precision nondestructive weak signal detection technology. In this paper, based on the size effect of an ultrahigh-quality (Q) calcium fluoride (CaF2) resonator, a weak acoustic signal is detected by the dispersive response regime in which an acoustic, elastic wave modulates the geometry and is converted to a resonance frequency shift. Through the structural design of the resonator, the sensitivity reaches 11.54 V/Pa at 10 kHz in the experiment. To our knowledge, the result is higher than that of other optical resonator acoustic sensors. We further detected a weak signal as low as 9.4 µPa/Hz1/2, which greatly improved the detection resolution. With a good directionality of 36.4 dB and a broadband frequency response range of 20 Hz–20 kHz, the CaF2 resonator acoustic sensing system can not only acquire and reconstruct speech signals over a long distance but also accurately identify and separate multiple voices in noisy environments. This system shows high performance in weak sound detection, sound source localization, sleep monitoring, and many other voice interaction applications.
Excitatory somatostatin interneurons in the dentate gyrus drive a widespread seizure network in cortical dysplasia
Authors: Zheng Yang , Xu Cenglin , Sun Jinyi , Ming Wenjie , Dai Sijie , Shao Yuying , Qiu Xiaoyun , Li Menghan , Shen Chunhong , Xu Jinghong , Fei Fan , Fang Jiajia , Jiang Xuhong , Zheng Guoqing , Hu Weiwei , Wang Yi , Wang Shuang , Ding Meiping , Chen Zhong 
Published: May 2023

AbstractSeizures due to cortical dysplasia are notorious for their poor prognosis even with medications and surgery, likely due to the widespread seizure network. Previous studies have primarily focused on the disruption of dysplastic lesions, rather than remote regions such as the hippocampus. Here, we first quantified the epileptogenicity of the hippocampus in patients with late-stage cortical dysplasia. We further investigated the cellular substrates leading to the epileptic hippocampus, using multiscale tools including calcium imaging, optogenetics, immunohistochemistry and electrophysiology. For the first time, we revealed the role of hippocampal somatostatin-positive interneurons in cortical dysplasia-related seizures. Somatostatin-positive were recruited during cortical dysplasia-related seizures. Interestingly, optogenetic studies suggested that somatostatin-positive interneurons paradoxically facilitated seizure generalization. By contrast, parvalbumin-positive interneurons retained an inhibitory role as in controls. Electrophysiological recordings and immunohistochemical studies revealed glutamate-mediated excitatory transmission from somatostatin-positive interneurons in the dentate gyrus. Taken together, our study reveals a novel role of excitatory somatostatin-positive neurons in the seizure network and brings new insights into the cellular basis of cortical dysplasia.
ABRAXAS1 orchestrates BRCA1 activities to counter genome destabilizing repair pathways—lessons from breast cancer patients
Authors: Sachsenweger Juliane , Jansche Rebecca , Merk Tatjana , Heitmeir Benedikt , Deniz Miriam , Faust Ulrike , Roggia Cristiana , Tzschach Andreas , Schroeder Christopher , Riess Angelika , Pospiech Helmut , Peltoketo Hellevi , Pylkäs Katri , Winqvist Robert , Wiesmüller Lisa 
Published: May 2023

AbstractIt has been well-established that mutations inBRCA1andBRCA2, compromising functions in DNA double-strand break repair (DSBR), confer hereditary breast and ovarian cancer risk. Importantly, mutations in these genes explain only a minor fraction of the hereditary risk and of the subset of DSBR deficient tumors. Our screening efforts identified two truncating germline mutations in the gene encoding the BRCA1 complex partner ABRAXAS1 in German early-onset breast cancer patients. To unravel the molecular mechanisms triggering carcinogenesis in these carriers of heterozygous mutations, we examined DSBR functions in patient-derived lymphoblastoid cells (LCLs) and in genetically manipulated mammary epithelial cells. By use of these strategies we were able to demonstrate that these truncatingABRAXAS1mutations exerted dominant effects on BRCA1 functions. Interestingly, we did not observe haploinsufficiency regarding homologous recombination (HR) proficiency (reporter assay, RAD51-foci, PARP-inhibitor sensitivity) in mutation carriers. However, the balance was shifted to use of mutagenic DSBR-pathways. The dominant effect of truncated ABRAXAS1 devoid of the C-terminal BRCA1 binding site can be explained by retention of the N-terminal interaction sites for other BRCA1-A complex partners like RAP80. In this case BRCA1 was channeled from the BRCA1-A to the BRCA1-C complex, which induced single-strand annealing (SSA). Further truncation, additionally deleting the coiled-coil region of ABRAXAS1, unleashed excessive DNA damage responses (DDRs) de-repressing multiple DSBR-pathways including SSA and non-homologous end-joining (NHEJ). Our data reveal de-repression of low-fidelity repair activities as a common feature of cells from patients with heterozygous mutations in genes encoding BRCA1 and its complex partners.
Mitophagy associated self-degradation of phosphorylated MAP4 guarantees the migration and proliferation responses of keratinocytes to hypoxia
Authors: Feng Yanhai , Li Lingfei , Zhang Qiong , He Yongqing , Huang Yao , Zhang Junhui , Zhang Dongxia , Huang Yuesheng , Lei Xia , Hu Jiongyu , Luo Gaoxing 
Published: May 2023

AbstractOur previous study has announced that phosphorylated microtubule-associated protein 4 (p-MAP4) accelerated keratinocytes migration and proliferation under hypoxia through depolymerizing microtubules. However, p-MAP4 should exhibit inhibitory effects on wound healing, for it also impaired mitochondria. Thus, figuring out the outcome of p-MAP4 after it impaired mitochondria and how the outcome influenced wound healing were far-reaching significance. Herein, the results revealed that p-MAP4 might undergo self-degradation through autophagy in hypoxic keratinocytes. Next, p-MAP4 activated mitophagy which was unobstructed and was also the principal pathway of its self-degradation triggered by hypoxia. Moreover, both Bcl-2 homology 3 (BH3) and LC3 interacting region (LIR) domains had been verified in MAP4, and they endowed MAP4 with the capability to synchronously function as a mitophagy initiator and a mitophagy substrate receptor. And, mutating any one of them ruined hypoxia-induced self-degradation of p-MAP4, resulting in destroyed proliferation and migration responses of keratinocytes to hypoxia. Our findings unviewed that p-MAP4 experienced mitophagy-associated self-degradation through utilizing its BH3 and LIR domains under hypoxia. As a result, the mitophagy-associated self-degradation of p-MAP4 guaranteed the migration and proliferation responses of keratinocytes to hypoxia. Together, this research provided a bran-new pattern of proteins in regulating wound healing, and offered a new direction for intervening wound healing.
Cellular population dynamics shape the route to human pluripotency
Authors: Panariello Francesco , Gagliano Onelia , Luni Camilla , Grimaldi Antonio , Angiolillo Silvia , Qin Wei , Manfredi Anna , Annunziata Patrizia , Slovin Shaked , Vaccaro Lorenzo , Riccardo Sara , Bouche Valentina , Dionisi Manuela , Salvi Marcello , Martewicz Sebastian , Hu Manli , Cui Meihua , Stuart Hannah , Laterza Cecilia , Baruzzo Giacomo , Schiebinger Geoffrey , Di Camillo Barbara , Cacchiarelli Davide , Elvassore Nicola 
Published: May 2023

AbstractHuman cellular reprogramming to induced pluripotency is still an inefficient process, which has hindered studying the role of critical intermediate stages. Here we take advantage of high efficiency reprogramming in microfluidics and temporal multi-omics to identify and resolve distinct sub-populations and their interactions. We perform secretome analysis and single-cell transcriptomics to show functional extrinsic pathways of protein communication between reprogramming sub-populations and the re-shaping of a permissive extracellular environment. We pinpoint the HGF/MET/STAT3 axis as a potent enhancer of reprogramming, which acts via HGF accumulation within the confined system of microfluidics, and in conventional dishes needs to be supplied exogenously to enhance efficiency. Our data suggest that human cellular reprogramming is a transcription factor-driven process that it is deeply dependent on extracellular context and cell population determinants.
Structural analysis and architectural principles of the bacterial amyloid curli
Authors: Sleutel Mike , Pradhan Brajabandhu , Volkov Alexander N. , Remaut Han 
Published: May 2023

AbstractTwo decades have passed since the initial proposition that amyloids are not only (toxic) byproducts of an unintended aggregation cascade, but that they can also be produced by an organism to serve a defined biological function. That revolutionary idea was borne out of the realization that a large fraction of the extracellular matrix that holds Gram-negative cells into a persistent biofilm is composed of protein fibers (curli; tafi) with cross-β architecture, nucleation-dependent polymerization kinetics and classic amyloid tinctorial properties. The list of proteins shown to form so-called functional amyloid fibers in vivo has greatly expanded over the years, but detailed structural insights have not followed at a similar pace in part due to the associated experimental barriers. Here we combine extensive AlphaFold2 modelling and cryo-electron transmission microscopy to propose an atomic model of curli protofibrils, and their higher modes of organization. We uncover an unexpected structural diversity of curli building blocks and fibril architectures. Our results allow for a rationalization of the extreme physico-chemical robustness of curli, as well as earlier observations of inter-species curli promiscuity, and should facilitate further engineering efforts to expand the repertoire of curli-based functional materials.
Rectifying disorder of extracellular matrix to suppress urethral stricture by protein nanofilm-controlled drug delivery from urinary catheter
Authors: Tian Juanhua , Fu Delai , Liu Yongchun , Guan Yibing , Miao Shuting , Xue Yuquan , Chen Ke , Huang Shanlong , Zhang Yanfeng , Xue Li , Chong Tie , Yang Peng 
Published: May 2023

AbstractUrethral stricture secondary to urethral injury, afflicting both patients and urologists, is initiated by excessive deposition of extracellular matrix in the submucosal and periurethral tissues. Although various anti-fibrotic drugs have been applied to urethral stricture by irrigation or submucosal injection, their clinical feasibility and effectiveness are limited. Here, to target the pathological state of the extracellular matrix, we design a protein-based nanofilm-controlled drug delivery system and assemble it on the catheter. This approach, which integrates excellent anti-biofilm properties with stable and controlled drug delivery for tens of days in one step, ensures optimal efficacy and negligible side effects while preventing biofilm-related infections. In a rabbit model of urethral injury, the anti-fibrotic catheter maintains extracellular matrix homeostasis by reducing fibroblast-derived collagen production and enhancing metalloproteinase 1-induced collagen degradation, resulting in a greater improvement in lumen stenosis than other topical therapies for urethral stricture prevention. Such facilely fabricated biocompatible coating with antibacterial contamination and sustained-drug-release functionality could not only benefit populations at high risk of urethral stricture but also serve as an advanced paradigm for a range of biomedical applications.