bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Rocks That Did Not Fall: A Multidisciplinary Analysis of Near‐Source Ground Motions From an Active Normal Fault
Authors: Trugman D. T. , Brune J. , Smith K. D. , Louie J. N. , Kent G. M. 
Published: April 2023

AbstractOn 8 July 2021 a M6.0 normal faulting earthquake rocked the community of Walker and the surrounding region near the California‐Nevada border. In the 1990s, field surveys of nearby Meadowcliff Canyon identified numerous precarious rocks deemed likely to topple in the event of strong shaking. Despite their proximity (∼6 km) to the 2021 earthquake, the precarious rocks still remain standing. In this work, we combine advanced source and ground motion characterization techniques to help unravel this mystery. High‐precision hypocentral locations reveal a clear north/south‐striking, east‐dipping rupture plane along the southern extension of the Slinkard Valley fault. The mainshock nucleated near the base of the fault, triggering thousands of aftershocks. Bayesian source spectral analyses indicate that the mainshock had a moderately‐high stress drop (∼17 MPa), and that aftershocks with deeper hypocenters have higher stress drops. Peak Ground Acceleration (PGA) recordings at regional stations agree well with existing ground motion models, predicting PGA of ∼0.3 g in Meadowcliff Canyon, a level sufficient to topple precarious rocks based on PGA‐derived stability criteria. We demonstrate that despite these large ground accelerations, the pulse duration in Meadowcliff Canyon is too short to supply the impulse necessary to damage these features, observations which support the application of dynamic toppling models that account for the joint effects of pulse amplitude and duration when assessing rock fragility. This study provides a unique vantage point from which to interpret rarely‐observed strong‐motion recordings from close to an active normal fault, one of many that dominate hazard along the eastern Sierra.
Strong Ocean Melting Feedback During the Recent Retreat of Thwaites Glacier
Authors: Holland Paul R. , Bevan Suzanne L. , Luckman Adrian J. 
Published: April 2023

AbstractAccelerating ice loss from Thwaites Glacier is contributing approximately 5% of global sea‐level rise, and could add tens of centimeters to sea level over the coming centuries. We use an ocean model to calculate sub‐ice melting for a succession of Digital Elevation Models of the main trunk of Thwaites Glacier from 2011 to 2022. The ice evolution during this period induces a strong geometrical feedback onto melting. Ice thinning and retreat provides a larger melting area, thicker and better‐connected sub‐ice water column, and steeper ice base. This leads to stronger sub‐ice ocean currents, increasing melting by over 30% without any change in forcing from wider ocean conditions. This geometrical feedback over just 12 years is comparable to melting changes arising from plausible century‐scale changes in ocean conditions and subglacial meltwater inflow. These findings imply that ocean‐driven ice loss from Thwaites Glacier may only be weakly influenced by anthropogenic emissions mitigation.
Reconstructing the Paleo‐Ecological Diet of Snow Petrels (Pagodroma nivea) From Modern Samples and Fossil Deposits: Implications for Southern Ocean Paleoenvironmental Reconstructions
Authors: Berg S. , Emmerson L. , Heim C. , Buchta E. , Fromm T. , Glaser B. , Hermichen W.‐D. , Rethemeyer J. , Southwell C. , Wand U. , Zech M. , Melles M. 
Published: April 2023

AbstractSnow petrels (Pagodroma nivea), which are endemic to the Antarctic region, produce proventricular stomach oil from ingested food for feeding purposes but also spit the oil in the immediate surrounds of the nests, where it forms encrustations over time (Antarctic mumiyo). These deposits provide a unique opportunity to understand the paleo‐ecological diet of snow petrels and because the seabirds forage in the ocean, they potentially provide an archive of past marine environmental conditions in the Southern Ocean. For validating methods for reconstructions we use compositional data obtained on modern stomach oils and DNA data from fecal samples of snow petrels. We find that the distribution of carboxylic acid compounds in modern stomach oils and in the fossil deposits are consistent with variable contributions of fish and krill, which are the main constituents of modern snow petrel diet, and allows inference of past changes in snow petrel diet from the fossil record. Analyses of mumiyo deposits from six regions in East Antarctica reveal systematic differences in the isotopic composition of organic matter (δ13C and δ15N) and carboxylic acid patterns. This may suggest regional and/or temporal variability in the composition of snow petrels diet, likely differing in response to the prevailing environmental conditions in the foraging range of the birds, such as sea‐ice variability, polynya activity and primary productivity. Our study provides confidence for using these approaches for broader scale paleo‐studies in the future and for an assessment of the temporal changes and regional variability in snow petrel diet.
Intact regulation of G1/S transition renders esophageal squamous cell carcinoma sensitive to PI3Kα inhibitors
Authors: Zhang Xu , Wang Yuxiang , Zhang Xi , Shen Yanyan , Yang Kang , Ma Qingyang , Qiao Yuemei , Shi Jiajie , Wang Yi , Xu Lan , Yang Biyu , Ge Gaoxiang , Hu Landian , Kong Xiangyin , Yang Chunhao , Chen Yi , Ding Jian , Meng Linghua 
Published: April 2023

AbstractPhosphatidylinositol 3-kinase alpha (PI3Kα) inhibitors are currently evaluated for the therapy of esophageal squamous cell carcinoma (ESCC). It is of great importance to identify potential biomarkers to predict or monitor the efficacy of PI3Kα inhibitors in an aim to improve the clinical responsive rate in ESCC. Here, ESCC PDXs with CCND1 amplification were found to be more sensitive to CYH33, a novel PI3Kα-selective inhibitor currently in clinical trials for the treatment of advanced solid tumors including ESCC. Elevated level of cyclin D1, p21 and Rb was found in CYH33-sensitive ESCC cells compared to those in resistant cells. CYH33 significantly arrested sensitive cells but not resistant cells at G1 phase, which was associated with accumulation of p21 and suppression of Rb phosphorylation by CDK4/6 and CDK2. Hypo-phosphorylation of Rb attenuated the transcriptional activation of SKP2 by E2F1, which in turn hindered SKP2-mediated degradation of p21 and reinforced accumulation of p21. Moreover, CDK4/6 inhibitors sensitized resistant ESCC cells and PDXs to CYH33. These findings provided mechanistic rationale to evaluate PI3Kα inhibitors in ESCC patients harboring amplified CCND1 and the combined regimen with CDK4/6 inhibitors in ESCC with proficient Rb.
Involvement and targeted intervention of benzo(a)pyrene-regulated apoptosis related proteome modification and muti-drug resistance in hepatocellular carcinoma
Authors: Yang Ye , Jin Ming , Meng Yajie , Dai Yi , Chen Shuai , Zhou Yan , Li Yuan , Tang Liming 
Published: April 2023

AbstractDuring the development of hepatocellular carcinoma (HCC), the mutual adaptation and interaction of HCC cells and the microenvironment play an important role. Benzo(a)pyrene (B[a]P) is a common environmental pollutant, which can induce the initiation of various malignant tumors, including HCC. However, the effects of B[a]P exposure on progression of HCC and the potential mechanisms remains largely uninvestigated. Here we found that, after the long-term exposure of HCC cells to low dose of B[a]P, it activated glucose-regulated protein 75 (GRP75), which then induced a modification of apoptosis-related proteome. Among them, we identified the X-linked inhibitor of apoptosis protein (XIAP) as a key downstream factor. XIAP further blocked the caspase cascade activation and promoted the acquisition of the anti-apoptosis abilities, ultimately leading to multi-drug resistance (MDR) in HCC. Furthermore, the abovementioned effects were markedly attenuated when we inhibited GRP75 by using 3,4-dihydroxycinnamic acid (caffeic acid, CaA). Collectively, our present study revealed the effects of B[a]P exposure on the progression of HCC, and identified GRP75 was a meaningful factor involved in.
Dynamically crossing diabolic points while encircling exceptional curves: A programmable symmetric-asymmetric multimode switch
Authors: Arkhipov Ievgen I. , Miranowicz Adam , Minganti Fabrizio , Özdemir Şahin K. , Nori Franco 
Published: April 2023

AbstractNontrivial spectral properties of non-Hermitian systems can lead to intriguing effects with no counterparts in Hermitian systems. For instance, in a two-mode photonic system, by dynamically winding around an exceptional point (EP) a controlled asymmetric-symmetric mode switching can be realized. That is, the system can either end up in one of its eigenstates, regardless of the initial eigenmode, or it can switch between the two states on demand, by simply controlling the winding direction. However, for multimode systems with higher-order EPs or multiple low-order EPs, the situation can be more involved, and the ability to control asymmetric-symmetric mode switching can be impeded, due to the breakdown of adiabaticity. Here we demonstrate that this difficulty can be overcome by winding around exceptional curves by additionally crossing diabolic points. We consider a four-mode $${{{{{{{\mathcal{PT}}}}}}}}$$
PT
-symmetric bosonic system as a platform for experimental realization of such a multimode switch. Our work provides alternative routes for light manipulations in non-Hermitian photonic setups.
Predictive and robust gene selection for spatial transcriptomics
Authors: Covert Ian , Gala Rohan , Wang Tim , Svoboda Karel , Sümbül Uygar , Lee Su-In 
Published: April 2023

AbstractA prominent trend in single-cell transcriptomics is providing spatial context alongside a characterization of each cell’s molecular state. This typically requires targeting an a priori selection of genes, often covering less than 1% of the genome, and a key question is how to optimally determine the small gene panel. We address this challenge by introducing a flexible deep learning framework, PERSIST, to identify informative gene targets for spatial transcriptomics studies by leveraging reference scRNA-seq data. Using datasets spanning different brain regions, species, and scRNA-seq technologies, we show that PERSIST reliably identifies panels that provide more accurate prediction of the genome-wide expression profile, thereby capturing more information with fewer genes. PERSIST can be adapted to specific biological goals, and we demonstrate that PERSIST’s binarization of gene expression levels enables models trained on scRNA-seq data to generalize with to spatial transcriptomics data, despite the complex shift between these technologies.
Low-dose IL-2 enhances the generation of IL-10-producing immunoregulatory B cells
Authors: Inaba Akimichi , Tuong Zewen Kelvin , Zhao Tian X. , Stewart Andrew P. , Mathews Rebeccah , Truman Lucy , Sriranjan Rouchelle , Kennet Jane , Saeb-Parsy Kourosh , Wicker Linda , Waldron-Lynch Frank , Cheriyan Joseph , Todd John A. , Mallat Ziad , Clatworthy Menna R. 
Published: April 2023

AbstractDysfunction of interleukin-10 producing regulatory B cells has been associated with the pathogenesis of autoimmune diseases, but whether regulatory B cells can be therapeutically induced in humans is currently unknown. Here we demonstrate that a subset of activated B cells expresses CD25, and the addition of low-dose recombinant IL-2 to in vitro stimulated peripheral blood and splenic human B cells augments IL-10 secretion. Administration of low dose IL-2, aldesleukin, to patients increases IL-10-producing B cells. Single-cell RNA sequencing of circulating immune cells isolated from low dose IL2-treated patients reveals an increase in plasmablast and plasma cell populations that are enriched for a regulatory B cell gene signature. The transcriptional repressorBACH2is significantly down-regulated in plasma cells from IL-2-treated patients, BACH2 binds to the IL-10 gene promoter, andBach2depletion or genetic deficiency increases B cell IL-10, implicating BACH2 suppression as an important mechanism by which IL-2 may promote an immunoregulatory phenotype in B cells.
Subtle tuning of nanodefects actuates highly efficient electrocatalytic oxidation
Authors: Gao Yifan , Liang Shuai , Liu Biming , Jiang Chengxu , Xu Chenyang , Zhang Xiaoyuan , Liang Peng , Elimelech Menachem , Huang Xia 
Published: April 2023

AbstractAchieving controllable fine-tuning of defects in catalysts at the atomic level has become a zealous pursuit in catalysis-related fields. However, the generation of defects is quite random, and their flexible manipulation lacks theoretical basis. Herein, we present a facile and highly controllable thermal tuning strategy that enables fine control of nanodefects via subtle manipulation of atomic/lattice arrangements in electrocatalysts. Such thermal tuning endows common carbon materials with record high efficiency in electrocatalytic degradation of pollutants. Systematic characterization and calculations demonstrate that an optimal thermal tuning can bring about enhanced electrocatalytic efficiency by manipulating the N-centered annulation–volatilization reactions and C-based sp3/sp2 configuration alteration. Benefiting from this tuning strategy, the optimized electrocatalytic anodic membrane successfully achieves >99% pollutant (propranolol) degradation during a flow-through (~2.5 s for contact time), high-flux (424.5 L m−2 h−1), and long-term (>720 min) electrocatalytic filtration test at a very low energy consumption (0.029 ± 0.010 kWh m−3 order−1). Our findings highlight a controllable preparation approach of catalysts while also elucidating the molecular level mechanisms involved.
Three dimensional architected thermoelectric devices with high toughness and power conversion efficiency
Authors: Karthikeyan Vaithinathan , Surjadi James Utama , Li Xiaocui , Fan Rong , Theja Vaskuri C. S. , Li Wen Jung , Lu Yang , Roy Vellaisamy A. L. 
Published: April 2023

AbstractFor decades, the widespread application of thermoelectric generators has been plagued by two major limitations: heat stagnation in its legs, which limits power conversion efficiency, and inherent brittleness of its constituents, which accelerates thermoelectric generator failure. While notable progress has been made to overcome these quintessential flaws, the state-of-the-art suffers from an apparent mismatch between thermoelectric performance and mechanical toughness. Here, we demonstrate an approach to potentially enhance the power conversion efficiency while suppressing the brittle failure in thermoelectric materials. By harnessing the enhanced thermal impedance induced by the cellular architecture of microlattices with the exceptional strength and ductility (>50% compressive strain) derived from partial carbonization, we fabricate three-dimensional (3D) architected thermoelectric generators that exhibit a specific energy absorption of ~30 J g−1 and power conversion efficiency of ~10%. We hope our work will improve future thermoelectric generator fabrication design through additive manufacturing with excellent thermoelectric properties and mechanical robustness.
Quantitative assessment can stabilize indirect reciprocity under imperfect information
Authors: Schmid Laura , Ekbatani Farbod , Hilbe Christian , Chatterjee Krishnendu 
Published: April 2023

AbstractThe field of indirect reciprocity investigates how social norms can foster cooperation when individuals continuously monitor and assess each other’s social interactions. By adhering to certain social norms, cooperating individuals can improve their reputation and, in turn, receive benefits from others. Eight social norms, known as the “leading eight," have been shown to effectively promote the evolution of cooperation as long as information is public and reliable. These norms categorize group members as either ’good’ or ’bad’. In this study, we examine a scenario where individuals instead assign nuanced reputation scores to each other, and only cooperate with those whose reputation exceeds a certain threshold. We find both analytically and through simulations that such quantitative assessments are error-correcting, thus facilitating cooperation in situations where information is private and unreliable. Moreover, our results identify four specific norms that are robust to such conditions, and may be relevant for helping to sustain cooperation in natural populations.
Expansion of circulating stem-like CD8+ T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in mice
Authors: Onyshchenko Kateryna , Luo Ren , Guffart Elena , Gaedicke Simone , Grosu Anca-Ligia , Firat Elke , Niedermann Gabriele 
Published: April 2023

AbstractCombination of radiation therapy (RT) with immune checkpoint blockade can enhance systemic anti-tumor T cell responses. Here, using two mouse tumor models, we demonstrate that adding long-acting CD122-directed IL-2 complexes (IL-2c) to RT/anti-PD1 further increases tumor-specific CD8+ T cell numbers. The highest increase (>50-fold) is found in the blood circulation. Compartmental analysis of exhausted T cell subsets shows that primarily undifferentiated, stem-like, tumor-specific CD8+ T cells expand in the blood; these cells express the chemokine receptor CXCR3, which is required for migration into tumors. In tumor tissue, effector-like but not terminally differentiated exhausted CD8+ T cells increase. Consistent with the surge in tumor-specific CD8+ T cells in blood that are migration and proliferation competent, we observe a CD8-dependent and CXCR3-dependent enhancement of the abscopal effect against distant/non-irradiated tumors and find that CD8+ T cells isolated from blood after RT/anti-PD1/IL-2c triple treatment can be a rich source of tumor-specific T cells for adoptive transfers.
A case study of percutaneous epidural stimulation to enable motor control in two men after spinal cord injury
Authors: Gorgey Ashraf S. , Trainer Robert , Sutor Tommy W. , Goldsmith Jacob A. , Alazzam Ahmed , Goetz Lance L. , Lester Denise , Lavis Timothy D. 
Published: April 2023

AbstractTwo persons with chronic motor complete spinal cord injury (SCI) were implanted with percutaneous spinal cord epidural stimulation (SCES) leads to enable motor control below the injury level (NCT04782947). Through a period of temporary followed by permanent SCES implantation, spinal mapping was conducted primarily to optimize configurations enabling volitional control of movement and training of standing and stepping as a secondary outcome. In both participants, SCES enabled voluntary increased muscle activation and movement below the injury and decreased assistance during exoskeleton-assisted walking. After permanent implantation, both participants voluntarily modulated induced torques but not always in the intended directions. In one participant, percutaneous SCES enabled motor control below the injury one-day following temporary implantation as confirmed by electromyography. The same participant achieved independent standing with minimal upper extremity self-balance assistance, independent stepping in parallel bars and overground ambulation with a walker. SCES via percutaneous leads holds promise for enhancing rehabilitation and enabling motor functions for people with SCI.
Assessing ecosystem vulnerability under severe uncertainty of global climate change
Authors: Yoshikawa Tetsuro , Koide Dai , Yokomizo Hiroyuki , Kim Ji Yoon , Kadoya Taku 
Published: April 2023

AbstractAssessing the vulnerability and adaptive capacity of species, communities, and ecosystems is essential for successful conservation. Climate change, however, induces extreme uncertainty in various pathways of assessments, which hampers robust decision-making for conservation. Here, we developed a framework that allows us to quantify the level of acceptable uncertainty as a metric of ecosystem robustness, considering the uncertainty due to climate change. Under the framework, utilizing a key concept from info-gap decision theory, vulnerability is measured as the inverse of maximum acceptable uncertainty to fulfill the minimum required goal for conservation. We applied the framework to 42 natural forest ecosystems and assessed their acceptable uncertainties in terms of maintenance of species richness and forest functional type. Based on best-guess estimate of future temperature in various GCM models and RCP scenarios, and assuming that tree species survival is primarily determined by mean annual temperature, we performed simulations with increasing deviation from the best-guess temperature. Our simulations indicated that the acceptable uncertainty varied greatly among the forest plots, presumably reflecting the distribution of ecological traits and niches among species within the communities. Our framework provides acceptable uncertainty as an operational metric of ecosystem robustness under uncertainty, while incorporating both system properties and socioeconomic conditions. We argue that our framework can enhance social consensus building and decision-making in the face of the extreme uncertainty induced by global climate change.
De novo full length transcriptome analysis of a naturally caffeine-free tea plant reveals specificity in secondary metabolic regulation
Authors: Mi Xiaozeng , Yang Chun , Qiao Dahe , Tang Mengsha , Guo Yan , Liang Sihui , Li Yan , Chen Zhengwu , Chen Juan 
Published: April 2023

AbstractTea plants are crops with economic, health and cultural value. Catechin, caffeine and theanine are the main secondary metabolites of taste. In the process of germplasm collection, we found a resource in the Sandu Aquatic Autonomous County of Guizhou (SDT) that possessed significantly different characteristic metabolites compared with the cultivar ‘Qiancha 1’. SDT is rich in theobromine and theophylline, possesses low levels of (−)-epicatechin-3-gallate, (−)-epigallocatechin-3-gallate, and theanine content, and is almost free of caffeine. However, research on this tea resource is limited. Full-length transcriptome analysis was performed to investigate the transcriptome and gene expression of these metabolites. In total, 78,809 unique transcripts were obtained, of which 65,263 were complete coding sequences. RNA-seq revealed 3415 differentially expressed transcripts in the tender leaves of ‘Qiancha 1’ and ‘SDT’. Furthermore, 2665, 6231, and 2687 differentially expressed transcripts were found in different SDT tissues. These differentially expressed transcripts were enriched in flavonoid and amino acid metabolism processes. Co-expression network analysis identified five modules associated with metabolites and found that genes of caffeine synthase (TCS) may be responsible for the low caffeine content in SDT. Phenylalanine ammonia lyase (PAL), glutamine synthetase (GS), glutamate synthase (GOGAT), and arginine decarboxylase (ADC) play important roles in the synthesis of catechin and theanine. In addition, we identified that ethylene resposive factor (ERF) and WRKY transcription factors may be involved in theanine biosynthesis. Overall, our study provides candidate genes to improve understanding of the synthesis mechanisms of these metabolites and provides a basis for molecular breeding of tea plant.