bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intermediate hepatitis B virus infection prevalence among 1622 pregnant women in rural Burkina Faso and implications for mother-to-child transmission
Authors: Ouoba Serge , Ko Ko , Lingani Moussa , Nagashima Shintaro , Guingané Alice N. , Bunthen E. , Hussain Md Razeen Ashraf , Sugiyama Aya , Akita Tomoyuki , Ohisa Masayuki , Sanou Moussa Abdel , Traore Ousmane , Nassa Job Wilfried , Sanou Maimouna , Takahashi Kazuaki , Tinto Halidou , Tanaka Junko 
Published: April 2023

AbstractIn highly endemic countries for hepatitis B virus (HBV) infection, childhood infection, including mother-to-child transmission (MTCT), represents the primary transmission route. High maternal DNA level (viral load ≥ 200,000 IU/mL) is a significant factor for MTCT. We investigated the prevalence of HBsAg, HBeAg, and high HBV DNA among pregnant women in three hospitals in Burkina Faso and assessed the performance of HBeAg to predict high viral load. Consenting pregnant women were interviewed on their sociodemographic characteristics and tested for HBsAg by a rapid diagnostic test, and dried blood spot (DBS) samples were collected for laboratory analyses. Of the 1622 participants, HBsAg prevalence was 6.5% (95% CI, 5.4–7.8%). Among 102 HBsAg-positive pregnant women in DBS samples, HBeAg was positive in 22.6% (95% CI, 14.9–31.9%), and viral load was quantified in 94 cases, with 19.1% having HBV DNA ≥ 200,000 IU/mL. HBV genotypes were identified in 63 samples and predominant genotypes were E (58.7%) and A (36.5%). The sensitivity of HBeAg by using DBS samples to identify high viral load in the 94 cases was 55.6%, and the specificity was 86.8%. These findings highlight the need to implement routine HBV screening and effective MTCT risk assessment for all pregnant women in Burkina Faso to enable early interventions that can effectively reduce MTCT.
Modulation of tau tubulin kinases (TTBK1 and TTBK2) impacts ciliogenesis
Authors: Bashore Frances M. , Marquez Ariana B. , Chaikuad Apirat , Howell Stefanie , Dunn Andrea S. , Beltran Alvaro A. , Smith Jeffery L. , Drewry David H. , Beltran Adriana S. , Axtman Alison D. 
Published: April 2023

AbstractTau tubulin kinase 1 and 2 (TTBK1/2) are highly homologous kinases that are expressed and mediate disease-relevant pathways predominantly in the brain. Distinct roles for TTBK1 and TTBK2 have been delineated. While efforts have been devoted to characterizing the impact of TTBK1 inhibition in diseases like Alzheimer’s disease and amyotrophic lateral sclerosis, TTBK2 inhibition has been less explored. TTBK2 serves a critical function during cilia assembly. Given the biological importance of these kinases, we designed a targeted library from which we identified several chemical tools that engage TTBK1 and TTBK2 in cells and inhibit their downstream signaling. Indolyl pyrimidinamine 10 significantly reduced the expression of primary cilia on the surface of human induced pluripotent stem cells (iPSCs). Furthermore, analog 10 phenocopies TTBK2 knockout in iPSCs, confirming a role for TTBK2 in ciliogenesis.
A machine learning approach to predict self-protecting behaviors during the early wave of the COVID-19 pandemic
Authors: Taye Alemayehu D. , Borga Liyousew G. , Greiff Samuel , Vögele Claus , D’Ambrosio Conchita 
Published: April 2023

AbstractUsing a unique harmonized real‐time data set from the COME-HERE longitudinal survey that covers five European countries (France, Germany, Italy, Spain, and Sweden) and applying a non-parametric machine learning model, this paper identifies the main individual and macro-level predictors of self-protecting behaviors against the coronavirus disease 2019 (COVID-19) during the first wave of the pandemic. Exploiting the interpretability of a Random Forest algorithm via Shapely values, we find that a higher regional incidence of COVID-19 triggers higher levels of self-protective behavior, as does a stricter government policy response. The level of individual knowledge about the pandemic, confidence in institutions, and population density also ranks high among the factors that predict self-protecting behaviors. We also identify a steep socioeconomic gradient with lower levels of self-protecting behaviors being associated with lower income and poor housing conditions. Among socio-demographic factors, gender, marital status, age, and region of residence are the main determinants of self-protective measures.
EPC-exosomal miR-26a-5p improves airway remodeling in COPD by inhibiting ferroptosis of bronchial epithelial cells via PTGS2/PGE2 signaling pathway
Authors: Liu Caihong , Lu Junjuan , Yuan Ting , Xie Lihua , Zhang Li 
Published: April 2023

AbstractWe aimed to investigate whether exosomes (Exo) affected chronic obstructive pulmonary disease (COPD) by influencing ferroptosis of bronchial epithelial cells (BECs) and the mechanisms involved. Here we took the peripheral blood samples of normal subjects and COPD patients, extracted and identified endothelial progenitor cells (EPCs) and EPC-Exo. An animal model of COPD was established. Then human BECs were taken and treated with cigarette smoke extract (CSE) for 24 h to construct a COPD cell model. Next, we screened differentially expressed ferroptosis-related genes in COPD patients by bioinformatics. Bioinformatics predicted the miRNA targeting PTGS2. Then, the mechanism of action of miR-26a-5p and Exo-miR-26a-5p was investigated in vitro. We successfully isolated and identified EPC and Exo. In vitro, EPC alleviated CSE-induced ferroptosis in BECs by transporting Exo. In vivo, Exo alleviated cigarette smoke-induced ferroptosis and airway remodeling in mice. Through further validation, we found that CSE-induced ferroptosis promoted the epithelial-mesenchymal transition (EMT) of BECs. Bioinformatics analysis and validation showed that PTGS2/PGE2 pathway affected CSE-induced ferroptosis in BECs. Meanwhile, miR-26a-5p targeting PTGS2 affected CSE-induced ferroptosis in BECs. Additionally, we found that miR-26a-5p affected CSE-induced BECs EMT. Exo-miR-26a-5p alleviated CSE-induced ferroptosis and EMT. In conclusion, EPC-exosomal miR-26a-5p improved airway remodeling in COPD by inhibiting ferroptosis of BECs via the PTGS2/PGE2 pathway.
Is blood-brain barrier a probable mediator of non-invasive brain stimulation effects on Alzheimer’s disease?
Authors: Petrovskaya Aleksandra , Tverskoi Artem , Medvedeva Angela , Nazarova Maria 
Published: April 2023

AbstractAlzheimer’s disease (AD) is a complex neurodegenerative disease with no existing treatment leading to full recovery. The blood-brain barrier (BBB) breakdown usually precedes the advent of first symptoms in AD and accompanies the progression of the disease. At the same time deliberate BBB opening may be beneficial for drug delivery in AD. Non-invasive brain stimulation (NIBS) techniques, primarily transcranial magnetic stimulation (TMS) and transcranial direct current stimulation (tDCS), have shown multiple evidence of being able to alleviate symptoms of AD. Currently, TMS/tDCS mechanisms are mostly investigated in terms of their neuronal effects, while their possible non-neuronal effects, including mitigation of the BBB disruption, are less studied. We argue that studies of TMS/tDCS effects on the BBB in AD are necessary to boost the effectiveness of neuromodulation in AD. Moreover, such studies are important considering the safety issues of TMS/tDCS use in the advanced AD stages when the BBB is usually dramatically deteriorated. Here, we elucidate the evidence of NIBS-induced BBB opening and closing in various models from in vitro to humans, and highlight its importance in AD.
Enhancement of zebrafish sperm production via a large body-sized surrogate with germ cell transplantation
Authors: Nayak Rigolin , Franěk Roman , Šindelka Radek , Pšenička Martin 
Published: April 2023

AbstractZebrafish (Danio rerio) is a commonly-used vertebrate model species for many research areas. However, its low milt volume limits effective cryopreservation of sperm from a single individual and often precludes dividing a single semen sample to conduct multiple downstream procedures such as genomic DNA/RNA extraction and in-vitro fertilization. Here, we apply germ stem cell transplantation to increase zebrafish sperm production in a closely related larger species from the same subfamily, giant danio Devario aequipinnatus. The endogenous germ cell of the host is depleted by dead-end morpholino antisense oligonucleotide. Histology of the sterile gonad and quantitative PCR of gonadal tissue reveals all sterile giant danio develop the male phenotype. Spermatogonial cells of Tg(ddx4:egfp) transgenic zebrafish are transplanted into sterile giant danio larvae, and 22% of recipients (germline chimera) produce donor-derived sperm at sexual maturation. The germline chimera produce approximately three-fold the volume of sperm and 10-fold the spermatozoon concentration of the donor. The donor-derived sperm is functional and gives rise to viable progeny upon fertilization of donor oocytes. We show that the issue of low milt volume can be effectively addressed by employing a larger surrogate parent.
Temporal nanofluid environments induce prebiotic condensation in water
Authors: de Herrera Andrea Greiner , Markert Thomas , Trixler Frank 
Published: April 2023

AbstractWater is a problem in understanding chemical evolution towards life’s origins on Earth. Although all known life is being based on water key prebiotic reactions are inhibited by it. The prebiotic plausibility of current strategies to circumvent this paradox is questionable regarding the principle that evolution builds on existing pathways. Here, we report a straightforward way to overcome the water paradox in line with evolutionary conservatism. By utilising a molecular deposition method as a physicochemical probe, we uncovered a synergy between biomolecule assembly and temporal nanofluid conditions that emerge within transient nanoconfinements of water between suspended particles. Results from fluorometry, quantitative PCR, melting curve analysis, gel electrophoresis and computational modelling reveal that such conditions induce nonenzymatic polymerisation of nucleotides and promote basic cooperation between nucleotides and amino acids for RNA formation. Aqueous particle suspensions are a geochemical ubiquitous and thus prebiotic highly plausible setting. Harnessing nanofluid conditions in this setting for prebiotic syntheses is consistent with evolutionary conservatism, as living cells also work with temporal nanoconfined water for biosynthesis. Our findings add key insights required to understand the transition from geochemistry to biochemistry and open up systematic pathways to water-based green chemistry approaches in materials science and nanotechnology.
The mechanistic diversity of the selective aerobic oxidation of alcohols catalyzed by systems derived from CuI and a diamine ligand
Authors: Zhong Wei , Luo Jiabin , Liu Zhenzhen , Zhan Guangli , Zhu Lihua , Lu Chunxin , Shen Zhongquan , Li Xueming , Liu Xiaoming 
Published: April 2023

UV-vis spectroscopy, cyclic voltammograms and tandem ESI-MS were employed to confirm that the selective aerobic oxidation of alcohols catalyzed by complex 1 involves mononuclear species, whereas complex 2 retains the binuclear core in the same reaction.
Catalytic asymmetric [4 + 1] cycloaddition to synthesize chiral pyrazoline-spirooxindoles
Authors: Hu Kaiqi , Zhang Dong , Wang Siyuan , Lin Lili , Feng Xiaoming 
Published: April 2023

The first asymmetric [4 + 1] annulation of oxindole 3-pyridinium salts and hydrazones is realized by using a chiral N,N′-dioxide-Pr(OTf)3 complex. A range of bioactive chiral pyrazoline-spirooxindoles are obtained in good yields with excellent ee values.
SNAP23 decreases insulin secretion by competitively inhibiting the interaction between SNAP25 and STX1A
Authors: Chen Jun , Wang Ziyan , Wang Tuanlao , Cheng Jidong , Zhuang Ruijuan , Wang Wei 
Published: April 2023

Abstract
SNAP25 is a core protein of the SNARE complex, which mediates stimulus-dependent secretion of insulin from the pancreatic β cells. SNAP23 is a SNAP25 homolog, however, the functional role of SNAP23 in the exocytic secretion of insulin is not known. Therefore, in the present study, we investigated the functional role of SNAP23 in the insulin secretory pathway. Our results demonstrated that over-expression of SNAP23 inhibited the secretion of insulin from the INS-1 cells. Conversely, SNAP23 depletion increased insulin secretion. Mechanistically, overexpression of SNAP23 decreased SNARE complex formation by blocking the binding of SNAP25 to STX1A. The full-length SNAP23 protein with the N-terminal and C-terminal SNARE binding domains was required for competition. Moreover, SNAP23 serine 95 phosphorylation plays a crucial function in insulin secretion by enhancing the interaction between SNAP23 and STX1A. The present study presents a new pathway regulating insulin secretion. Therefore, SNAP23 may be a potential therapeutic target for diabetes mellitus.
Social Determinants of Health: Implications for Voice Disorders and Their Treatment
Authors: Mayne Geneva V. , Namazi Mahchid 
Published: April 2023

Purpose:
Adverse childhood experiences (ACEs) and related conditions, such as discrimination, are social determinants of health (SDOHs). Critical race theory (CRT) is a lens through which to understand SDOHs in a way that may impact our clinical care. When prolonged or chronic, SDOHs might cause toxic stress and trauma, which, in turn, adversely affect health and are shown to be relevant for some voice disorders. The goals of this tutorial are to (a) review the literature on SDOHs that potentially contribute to disparities; (b) discuss explanatory models and theories that describe how psychosocial factors influence health; (c) relate the foregoing information to voice disorders, highlighting functional voice disorders (FVDs) as a particular case in point; and (d) describe how trauma-informed care can improve patient outcomes and advance health equity for vulnerable populations.

Conclusions:
This tutorial concludes with a call for heightened awareness of the role that SDOHs such as structural and individual discrimination may play in voice disorders, and a call for research into SDOHs, traumatic stress, and health disparities in this patient population. A call is also made for more universal practice of trauma-informed care in the clinical voice domain.
Triangular lattice formation in robot swarms with minimal local sensing
Authors: Nie Zisen , Zhang Qingrui , Wang Xiaohan , Wang Fakui , Hu Tianjiang 
Published: April 2023

AbstractThe problem of triangular lattice formation in robot swarms has been investigated extensively in the literature, but the existing algorithms can hardly keep comparative performance from swarm simulation to real multi‐robot scenarios, due to the limited computation power or the restricted field of view (FOV) of robot sensors. Eventually, a distributed solution for triangular lattice formation in robot swarms with minimal sensing and computation is proposed and developed in this study. Each robot is equipped with a sensor with a limited FOV providing only a ternary digit of information about its neighbouring environment. At each time step, the motion command is directly determined by using only the ternary sensing result. The circular motions with a certain level of randomness lead the robot swarms to stable triangular lattice formation with high quality and robustness. Extensive numerical simulations and multi‐robot experiments are conducted. The results have demonstrated and validated the efficiency of the proposed approach. The minimised sensing and computation requirements pave the way for massive deployment at a low cost and implementation within swarms of miniature robots.
Milky Way globular clusters on cosmological timescales
Authors: Ishchenko Maryna , Sobolenko Margaryta , Berczik Peter , Khoperskov Sergey , Omarov Chingis , Sobodar Olexander , Makukov Maxim 
Published: April 2023

Context. Recent observational data show that the Milky Way galaxy contains about 170 globular clusters. A fraction of them is likely formed in dwarf galaxies that were accreted onto the Milky Way in the past, while the remaining clusters were formed in situ. Therefore, the different parameters, including the orbits, of the globular clusters are a valuable tool for studying the Milky Way evolution. However, because the evolution of the 3D mass distribution of the Milky Way is poorly constrained, the orbits of the clusters are usually calculated in static potentials.
Aims. We study the evolution of the globular clusters in several external potentials, where we aim to quantify the effects of the evolving galaxy potential on the orbits of the globular clusters.
Methods. For the orbit calculation, we used five Milky Way-like potentials from the IllustrisTNG-100 simulation. The orbits of 159 globular clusters were integrated using the high-order N-body parallel dynamic code φ-GPU, with initial conditions obtained from the recent Gaia Data Release 3 catalogues.
Results. We provide a classification of the globular cluster orbits according to their 3D shapes and association with different components of the Milky Way (disk, halo, and bulge). We also found that the energy – angular momentum of the globular clusters in the external potentials is roughly similarly distributed at the present time. However, neither total energy nor total angular momentum of the globular clusters are conserved due to time-varying nature of the potentials. In some extreme cases, the total energy can change up to 40% (18 objects) over the last 5 Gyr of evolution. We found that the in situ formed globular clusters are less affected by the evolution of the TNG potentials than clusters that were likely formed ex situ. Therefore, our results suggest that time-varying potentials significantly affect the orbits of the globular clusters, thus making them vital for understanding the formation of the Milky Way.
Āyurveda in der ärztlichen Praxis – ein Fallbeispiel
Authors: Gupta Hedwig 
Published: April 2023

ZusammenfassungDer Āyurveda ist ein umfassendes traditionelles Medizinsystem aus dem indischen Subkontinent. Er hat eigene Vorstellungen zur Physiologie, Ätiologie und Pathogenese von Erkrankungen und besitzt ein differenziertes Therapiesystem mit breit aufgestellten Behandlungsmöglichkeiten. Jede Störung – egal, ob körperlich, geistig oder emotional – kann mittels dieses Systems diagnostiziert und kausal behandelt werden. Dabei ist es insbesondere bei solchen Erkrankungen indiziert, indem eine polyätiologische Situation vorliegt. Wie der Beitrag am Beispiel einer Patientin mit Depression und chronischem muskuloskelettalen Schmerz zeigt, kann der Āyurveda leicht in die ärztliche Praxis integriert und erfolgreich umgesetzt werden.
Plasma exchange in inflammatory demyelinating disorders of the central nervous system: reasonable use in the clinical practice
Authors: Queiroz André Luiz Guimarães de , Soares Neto Herval Ribeiro , Kobayashi Thiago Taya , Silva Sonia Maria Cesar de Azevedo 
Published: April 2023

AbstractPlasma exchange (PLEX) is a therapeutic apheresis modality in which the plasma is separated from inflammatory factors such as circulating autoreactive immunoglobulins, the complement system, and cytokines, and its therapeutic effect is based on the removal of these mediators of pathological processes. Plasma exchange is well established for various neurological disorders, and it is applied successfully in central nervous system inflammatory demyelinating diseases (CNS-IDD). It mainly modulates the humoral immune system; thus, it has a greater theoretical effect in diseases with prominent humoral mechanisms, such as neuromyelitis optica (NMO). However, it also has a proven therapeutic effect in multiple sclerosis (MS) attacks. Several studies have suggested that patients with severe attacks of CNS-IDD have poor response to steroid therapy but show clinical improvement after the PLEX treatment. Currently, PLEX is generally established only as a rescue therapy for steroid unresponsive relapses. However, there are still research gaps in the literature regarding plasma volume, number of sessions, and how early the apheresis treatment needs to started. Thus, in the present article, we summarize the clinical studies and meta-analyses, especially about MS and NMO, outlining clinical data regarding the experience with therapeutic PLEX in severe attacks of CNS-IDD, the clinical improvement rates, the prognostic factors of a favorable response, and highlighting the likely role of the early apheresis treatment. Further, we have gathered this evidence and suggested a protocol for the treatment of CNS-IDD with PLEX in the routine clinical practice.
Elderly people with human T-cell leukemia virus type 1-associated myelopathy present an early impairment in cognitive skills
Authors: Sousa Beatriz Rezende Matos de , Labanca Ludimila , Diniz Maria Luiza , Rausse Nathália de Castro Botini , Gonçalves Denise Utsch 
Published: April 2023

Abstract
Background Cerebral changes occur in individuals with human T-cell leukemia virus type 1 (HTLV-1)-associated myelopathy (HAM) and seem to predominate in subcortical areas. Little is known about the cognitive decline in the elderly living with HTLV-1.
Objective To evaluate the cognitive aging of individuals infected with HTLV-1 aged ≥ 50 years.
Methods This is a cross-sectional study of former blood donors infected with HTLV-1 who have been followed in the cohort of the Interdisciplinary Research Group on HTLV-1 since 1997. The groups of study consisted of 79 HTLV-1 infected individuals aged ≥ 50 years, with 41 of them presenting symptomatic HAM and 38 being asymptomatic carriers, and 59 seronegative individuals (controls) aged ≥ 60 years. All were submitted to the P300 electrophysiological test and neuropsychological tests.
Results Individuals with HAM presented delayed P300 latency in relation to the other groups, and this latency delay increased progressively with aging. The performance of this group in the neuropsychological tests was also the worst. The HTLV-1- asymptomatic group performance was similar to that of the control group.
Conclusions Individuals with HAM presented cognitive decline that progressed with aging and, although HTLV-1-asymptomatic carriers appear to present cognitive aging similar to that of healthy elderly people, concern about a subclinical cognitive impairment is warranted in this population.
Acute headache attributed to ischemic stroke: assessment of its characteristics and associated factors
Authors: Oliveira Felipe Araújo Andrade de , Dourado-Filho Mário Genuíno , Rocha-Filho Pedro Augusto Sampaio 
Published: April 2023

Abstract
Background It is estimated that headache attributed to ischemic stroke occurs in 7.4% to 34% of the cases. Despite its frequency, this headache has been little studied in terms of its risk factors and characteristics.
Objective To assess the frequency and clinical characteristics of headache attributed to ischemic stroke and the factors associated with its occurrence.
Methods The present was a cross-sectional study which included patients consecutively admitted within 72 hours of the onset of ischemic stroke. A semi-structured questionnaire was used. The patients underwent magnetic resonance imaging.
Results A total of 221 patients were included, 68.2% of whom were male, and the mean age was of 68.2 ±  13.8 years. The frequency of headache attributed to ischemic stroke was of 24.9% (95% confidence interval [95%CI]: 19.6–31.1%). The headache had a median duration of 21 hours and most frequently began at the same time as the focal deficit (45.3%), with a gradual onset (83%). It was of moderate intensity, pulsatile (45.3%), bilateral (54.6%), and presented a similar pattern to that of tension-type headache (53.6%). Headache attributed to stroke was significantly associated with previous tension-type headache, and previous migraine with and without aura (logistic regression).
Conclusion Headache attributed to stroke is common, with a pattern similar to that of tension-type headache, and it is associated with a history of tension-type and migraine headaches.