bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular characterization of the nearly complete nuclear ribosomal DNA sequence of the Neotropical freshwater copepod Arctodiaptomus dorsalis (Marsh, 1907) (Copepoda, Calanoida, Diaptomidae)
Authors: Guinto S. K. P. , Machida R. J. , Papa R. D. S. 
Published: May 2023

Abstract
Arctodiaptomus dorsalis has been reported in the Philippines as an invasive species, which originated from Central America. Apart from its wide distribution in the United States and Central America, it is now widely distributed in many lakes in the Philippine islands, such as Luzon Island and southwestern Mindanao, making it an ideal specimen for studies on population dynamics and population genetics. Recently, new techniques have been emerging as important zooplankton monitoring tools, such as metabarcoding and the use of environmental DNA/environmental RNA. However, these would only be useful if good-quality reference DNA sequences are available before utilizing these techniques. This study sequenced the nearly complete nuclear ribosomal DNA (nrDNA) of A. dorsalis from 202 individuals from Lake Taal, Batangas. The total length was 5887 bp, which includes the partial 18S sequences, and the complete 5.8S, ITS and 28S sequences with an organization that is typical for any eukaryotic organism. This is the first record of a nearly complete nrDNA sequence for freshwater calanoid copepods and is considered an important stepping stone for further molecular studies in freshwater Copepoda.
Eriocheir hepuensis Dai, 1991 (Brachyura, Grapsoidea, Varunidae, Varuninae): reporting the southerly distribution to northern Vietnam
Authors: Clark Paul F. , Ngô Văn Trí , Ng Ngan Kee , Gan Lydia Xinjie , Kamanli Seyit Ali , Ng Peter K. L. 
Published: May 2023

Abstract
Eriocheir hepuensis Dai, 1991, the Hepu mitten crab, is here formally reported from Thanh Hoa and Quang Ninh Provinces, northern Vietnam. Previous reports of “E. sinensis” and “E. japonicus” from “Cua lông”, Thai Binh Province, northern Vietnam, require clarification, are probably misidentifications of E. hepuensis, and highlight the problems of species identification within Eriocheir. The taxonomy of E. hepuensis is also discussed.
The Limits of Abstraction: Towards a Phenomenologically Reformed Understanding of Science
Authors: Berghofer Philipp 
Published: May 2023

Abstract
Husserl argued that psychology needs to establish an abstraction that is opposite to the abstraction successfully established in the natural sciences. While the natural sciences abstract away the psychological or subjective, psychology must abstract away the physical or worldly. However, Husserl and other phenomenologists such as Iso Kern have argued that there is a crucial systematic disanalogy between both abstractions. While the abstraction of the natural sciences can be performed completely, the abstraction of psychology cannot. In this context, Husserl argues that the psychological reduction leads to paradoxes. In this paper, I critically discuss whether it is true that the natural sciences can successfully abstract away the subjective. Or more precisely, I raise the question of whether they should.
CircPrime: a web-based platform for design of specific circular RNA primers
Authors: Sharko Fedor , Rbbani Golam , Siriyappagouder Prabhugouda , Raeymaekers Joost A. M. , Galindo-Villegas Jorge , Nedoluzhko Artem , Fernandes Jorge M. O. 
Published: May 2023

Abstract
Background
Circular RNAs (circRNAs) are covalently closed-loop RNAs with critical regulatory roles in cells. Tens of thousands of circRNAs have been unveiled due to the recent advances in high throughput RNA sequencing technologies and bioinformatic tools development. At the same time, polymerase chain reaction (PCR) cross-validation for circRNAs predicted by bioinformatic tools remains an essential part of any circRNA study before publication.

Results
Here, we present the CircPrime web-based platform, providing a user-friendly solution for DNA primer design and thermocycling conditions for circRNA identification with routine PCR methods.

Conclusions
User-friendly CircPrime web platform (http://circprime.elgene.net/) works with outputs of the most popular bioinformatic predictors of circRNAs to design specific circular RNA primers. CircPrime works with circRNA coordinates and any reference genome from the National Center for Biotechnology Information database).
GraphSNP: an interactive distance viewer for investigating outbreaks and transmission networks using a graph approach
Authors: Permana Budi , Beatson Scott A. , Forde Brian M. 
Published: May 2023

Abstract
Background
Cluster and transmission analysis utilising pairwise SNP distance are increasingly used in genomic epidemiological studies. However, current methods are often challenging to install and use, and lack interactive functionalities for easy data exploration.

Results
GraphSNP is an interactive visualisation tool running in a web browser that allows users to rapidly generate pairwise SNP distance networks, investigate SNP distance distributions, identify clusters of related organisms, and reconstruct transmission routes. The functionality of GraphSNP is demonstrated using examples from recent multi-drug resistant bacterial outbreaks in healthcare settings.

Conclusions
GraphSNP is freely available at https://github.com/nalarbp/graphsnp. An online version of GraphSNP, including demonstration datasets, input templates, and quick start guide is available for use at https://graphsnp.fordelab.com.
Association between sleep parameters and chronic kidney disease: findings from iranian ravansar cohort study
Authors: Hemati Niloofar , Shiri Farshad , Ahmadi Farrokhlegha , Najafi Farid , Moradinazar Mehdi , Norouzi Ebrahim , Khazaie Habibolah 
Published: May 2023

Abstract
Introduction
The relationship between sleep duration and chronic kidney disease (CKD) has received relatively little attention in the Kurdish community. Considering the ethnic diversity of Iran and the importance of the Kurdish community, the present study investigated the association between sleep parameters and CKD among a large sample of Iranian-Kurds.

Methods
This cross-sectional study was conducted among 9,766 participants (Mage: 47.33, SD = 8.27, 51% female) from the Ravansar Non Communicable Disease (RaNCD) cohort study database. Logistic regression analyses were applied to examine the association between sleep parameters and CKD.

Results
Results showed that prevalence of CKD was detected in 1,058 (10.83%) individuals. Time to fall asleep (p = 0.012) and dozing off during the day (p = 0.041) were significantly higher in the non-CKD group compared to the CKD group. Daytime napping and dozing off during the day in females with CKD were significantly more than males with CKD. A long sleep duration (> 8 h/day) was associated with 28% (95% CI: 1.05, 1.57) higher odds of CKD compared to normal sleep duration (7 h/d), after adjusting for confounding factors. Participants who experienced leg restlessness had a 32% higher probability of developing CKD than those who did not experience leg restlessness (95% CI: 1.03, 1.69).

Conclusion
Results suggest that sleep duration and leg restlessness may be associated with an increased likelihood of CKD. Consequently, regulating sleep parameters may play a role in improving sleep and preventing CKD.
CCL19/CCR7 drives regulatory T cell migration and indicates poor prognosis in gastric cancer
Authors: Xu Danhua , Liu Xu , Ke Shouyu , Guo Yixian , Zhu Chunchao , Cao Hui 
Published: May 2023

Abstract
Background
Gastric cancer is associated with significant morbidity and mortality in the world. Blocking programmed cell death protein 1 pathway have been approved for the treatment of a variety of tumors and have achieved remarkable clinical therapeutic effects. However, immune checkpoint inhibitors failed to achieve satisfactory results in gastric cancer. There is a need to identify novel immunotherapy targets in gastric cancer.

Methods
We analysed the correlation between Treg cells and CD8 + T cells in gastric cancer samples. We studied the relationship between chemokines and Treg cells or CD8 + T cells in gastric cancer. We compared CCL19/CCR7 expression in gastric cancer patients in TCGA database. We performed transwell experiments to determine the influence of CCL19 on Treg cells and CD8 + T cells migratory capacity. We conducted survival analysis of CCL19 and CCR7 in gastric cancer database.

Results
Treg cells show positive correlation with CD8 + T cells in gastric cancer. Treg cell expression was significantly upregulated in tumor tissues. Patients with high FOXP3 expression had worse overall survival than those with low FOXP3 expression. CCL19 had strong correlation with FOXP3 and weak correlation with CD8A. CCL19 had strong impact on the migratory capacity of Treg cells but weak impact on the migratory capacity of CD8 + T cells. Both CCL19 and CCR7 expression were significantly upregulated in gastric cancer tissues. Survival analysis demonstrated that both CCL19 and CCR7 indicate poor prognosis in gastric cancer.

Conclusions
CCL19/CCR7 may be a potential novel therapeutic target in gastric cancer.
Symmetric offset versus asymmetric offset ablation with transepithelial refractive keratectomy
Authors: de Ortueta Diego , von Rüden Dennis , Arba Mosquera Samuel 
Published: May 2023

Abstract
Background
In eyes with hyperopia, astigmatism, and mixed astigmatism Transepithelial photorefractive keratectomy (TransPRK) is a modality of surface ablation surgery. We center on the corneal vertex for all our treatments (all have an offset to the center of the pupil) and wanted to compare the visual results of symmetrical profile treatments versus asymmetrical profile treatments (the center of the treatment on the vertex and the boundaries with the pupil center) using TransPRK as corneal refractive surgery.

Methods
We retrospectively analyzed two consecutive groups of eyes treated with TransPRK in the Aurelios Augenlaserzentrum Recklinghausen: 47 eyes treated with symmetrical offset and 51 eyes treated with asymmetrical offset. The intergroup comparisons were assessed using unpaired Student’s T-tests, whereas preoperative to postoperative changes were assessed using paired Student’s T-tests.

Results
Refractive outcomes were good for both groups. 83 and 88% of eyes were within the spherical equivalent of 0.5 D from the target in the symmetric and asymmetric offset groups, respectively. 85 and 84% of eyes had a postoperative astigmatism of 0.5 D or lower in the symmetric and asymmetric offset groups, respectively.

Conclusion
We have not found a significant difference in the refractive outcomes between the symmetric group and the asymmetric group of eyes treated both with TransPRK for preoperatively hyperopic or mixed astigmatism.
Pulmonary syphilis with a cicatricial variant of organizing pneumonia: a case report
Authors: Goda Ken , Katsurada Masahiro , Doi Takefumi , Saga Nobuyuki , Maniwa Yoshimasa , Kenzaka Tsuneaki 
Published: May 2023

Abstract
Background
Syphilis is a chronic disease that progresses in the primary, secondary, latent, and tertiary stages. Pulmonary manifestations of syphilis are rare, and their histological features have not been well-described.

Case presentation
A 78-year-old man was referred to our hospital because of a solitary nodular shadow in the right middle lung field on a chest radiograph. Five years prior, a rash appeared on both legs. He was tested for syphilis at a public health center, and the non-treponemal test result was negative. When he was approximately 35 years old, he had unspecified sexual intercourse. Chest computed tomography showed a 13-mm nodule with a cavity in S6 of the right lower lobe of the lung. Robot-assisted resection of the right lower lobe was performed because of suspected localized right lower lobe lung cancer. A cicatricial variant of organizing pneumonia (CiOP) was observed, and immunohistochemistry identified Treponema pallidum inside the macrophages in the nodule cavity. The rapid plasma regain (RPR) value was negative, and the Treponema pallidum hemagglutination assay was positive. The patient was diagnosed as having secondary syphilis with pulmonary involvement. Insidious progression of secondary syphilis may result in CiOP and a negative RPR test result.

Conclusions
We report the first case of pulmonary syphilis with a histological pattern of CiOP. It may be asymptomatic and difficult to diagnose because the RPR test may be negative for a long period of time. When either non-treponemal or treponemal test results are positive, the possibility of pulmonary syphilis should be considered along with appropriate medical treatment.
Effect of Kinesio tape and Compression sleeves on delayed onset of muscle soreness: a single-blinded randomized controlled trial
Authors: Xue Xiali , Hao Yuerong , Yang Xinwei , Zhang Chaoyang , Xu Jie , Wu Xiaolei , Deng Zhongyi , Li Ning 
Published: May 2023

Abstract
Background
Both Kinesio Tape (KT) and Compression Sleeves (CS) can relieve Delayed Onset Muscle Soreness (DOMS) to a certain extent, but there is no study report on the difference in the effectiveness of the KT and CS whether the effect is better when used at the same time. The purpose of this study was to compare the effects of KT and CS on the recovery of muscle soreness, isokinetic strength, and body fatigue after DOMS.

Methods
In this single-blinded randomized controlled trial, 32 participants aged 18 to 24 years were randomly divided into Control group (CG), Compression Sleeves group (CSG), Kinesio Tape group (KTG), Compression Sleeves and Kinesio Tape group (CSKTG), between October 2021 and January 2022. KTG uses Kinesio Tape, CSG wears Compression Sleeves, and CSKTG uses both Compression Sleeves and Kinesio Tape. Outcomes were performed at five-time points (baseline, 0 h, 24 h, 48 h, 72 h), Primary outcome was pain level Visual Analogue Scale (VAS), and Secondary outcomes were Interleukin 6, Peak Torque/Body Weight, Work Fatigue. Statistical analyses were performed using the repeated measures analysis of variance method. Setting: Laboratory.

Results
After the intervention, VAS reached the highest at 24 h after exercise-induced muscle soreness, while the KTG and CSG at each time point were less than CG, and the scores of CSKTG at 24 h and 48 h were less than those of KTG and CSG in the same period (P < 0.05). Interleukin 6, at 24 h, CSKTG is lower than KTG 0.71(95%CI: 0.43 to 1.86) and CG 1.68(95%CI: 0.06 to 3.29). Peak Torque/Body Weight, at 24 h, CG was lower than CSKTG 0.99(95%CI: 0.42 to 1.56), KTG 0.94(95%CI: 0.37 to 1.52), and CSG 0.72(95%CI: 0.14 to 1.29); at 72 h, CG was lower than CSKTG 0.65(95%CI: 0.13 to 1.17) and KTG 0.58(95%CI: 0.06 to 1.10). Work Fatigue, at 24 h, CG was lower than KTG 0.10(95%CI: 0.02 to 1.78) and CSKTG 0.01(95%CI: -0.07 to 0.09). At 48 h, CG was lower than KTG 0.10(95%CI: 0.13 to 1.17) and CSKTG 0.11(95%CI: 0.03 to 0.18).

Conclusions
Kinesio Tape can significantly reduce DOMS pain, and Kinesio Tape has a better recovery effect on Delayed Onset Muscle Soreness than Compression Sleeves. Kinesio Tape combined with Compression Sleeves is helpful to alleviate the Delayed Onset Muscle Soreness pain, speeding up the recovery of muscle strength, and shortening the recovery time after Delayed Onset Muscle Soreness.

Trial registration
Registration number: This study was also registered on 11/10/2021, at the Chinese Clinical Trial Registry (ChiCTR2100051973).
Surgical management outcome and its associated factors among intestinal obstruction patients admitted to adult surgical ward of Wollega University Referral Hospital, Ethiopia
Authors: Shama Adisu Tafari , Terefa Olana , Gadisa Iyasu , Feyera Gemechu , Tamiru Getnet , Terefa Dufera Rikitu , Merdassa Emiru 
Published: May 2023

Abstract
Background
Globally, bowel obstruction is the most common cause of surgical emergencies. It remains a challenge to healthcare workers in spite of improvements in management techniques. There is a lack of the study to determine the surgical management outcome and its associated factors in the area of study. Hence, this study aimed to determine management outcome and its associated factors among surgically treated intestinal obstruction patients at Wollega University Referral Hospital, 2021.

Methods
Facility-based cross-sectional study was conducted among all cases surgically managed for intestinal obstruction between September 1, 2018 and September 1, 2021. Data were collected using a structured checklist. The collected data were checked for completeness and entered into data entry software and then exported to SPSS version 24 for data cleaning and analysis. Both bi-variable and multivariable logistic regressions were run. P-value < 0.05 was used to declare a statistically significant association in multivariable logistic regression. The odds ratio along with 95%CI was estimated to measure the strength of the association.

Result
116 (59.2%) patients had favorable surgical management outcome for intestinal obstruction. Male sex (AOR = 3.694;95%CI:1.501,9.089), absence of fever (AOR = 2.636; 95%CI:1.124,6.18), ≤ 48 h duration of illness before operation (AOR = 3.045; 95%CI:1.399,6.629), viable intraoperative bowel condition (AOR = 2.372; 95%CI:1.088, 5.175), having bowel resection and anastomosis (AOR = 0.234; 95%CI:0.101,0.544) were the significantly associated factors of the favorable surgical management outcome for intestinal obstruction.

Conclusion and recommendation
The favorable management outcome of patients with intestinal obstruction who were treated surgically in this study was low. Factors like sex, fever, short duration of illness, viable intraoperative bowel condition, and bowel resection and anastomosis were found to influence the surgical management outcome of patients with intestinal obstruction. The patient with intestinal obstruction should seek health care on time. Health professionals have to be skilled and provide appropriate care for the patients to reduce the risk of complications.
The effect of short-term training about depth predicting score on the diagnostic ability of invasion depth for differentiated early gastric Cancer among non-expert endoscopists
Authors: Li Hui , Hu Hui , Geng Ping , Guo Panhui , Zhu Yuanrong , Zeng Lulu , Liu Jun , Hu Xiangpeng 
Published: May 2023

Abstract
Background
The depth-predicting score (DPS) was proposed based on conventional white-light imaging (C-WLI) endoscopic features of early gastric cancer (EGC) to determine the invasion depth of the neoplasm. However, the effect of DPS on training endoscopists remains unclear. Therefore, we aimed to investigate the effect of short-term DPS training on improving the diagnostic ability of EGC invasion depth and compare the training effect among non-expert endoscopists at different levels.

Methods
In the training session, the definitions and scoring rules of DPS were instructed, and classic C-WLI endoscopic example graphics were exhibited to the participants. Another C-WLI endoscopic images of 88 cases of histologically proven differentiated EGC were selected as an independent test dataset for evaluating the training effect. Each participant was tested, and the diagnostic accuracy rate of invasion depth was calculated differently one week before the training and after the completion of training.

Results
A total of 16 participants were enrolled and completed the training. Participants were divided into a trainee group and a junior endoscopist group according to the total number of C-WLI endoscopies performed. The total number of C-WLI endoscopies performed showed a significant difference between the trainee group and junior endoscopist group (350 vs. 2500, P = 0.001). No significant difference between the trainee group and junior endoscopist group was observed for pre-training accuracy. The overall diagnostic accuracy of invasion depth was improved significantly after completing DPS training compared with before (68.75 ± 5.71% vs. 61.58 ± 9.61%, P = 0.009). In the subgroup analysis, the post-training accuracy was higher than the pre-training accuracy, but significant improvement was observed only in the trainee group (61.65 ± 7.33% vs. 68.32 ± 5.71%, P = 0.034). In addition, no significant difference in post-training accuracy between the two groups was observed.

Conclusion
Short-term DPS training can improve the diagnostic ability of the invasion depth of EGC and homogenize the diagnostic ability of non-expert endoscopists at different levels. The depth-predicting score was convenient and effective for endoscopist training.
Psychological distress and academic self-efficacy of nursing undergraduates under the normalization of COVID-19: multiple mediating roles of social support and mindfulness
Authors: Xu Ting , Zhu Pingting , Ji Qiaoying , Wang Wen , Qian Meiyan , Shi Guanghui 
Published: May 2023

AbstractBackgroundNursing undergraduates’ academic self-efficacy is a significant factor in determining their learning motivation, cognition, and emotions. It has a significant impact on improving academic performance and achieving learning goals.MethodsTo explore the mechanism of psychological distress affecting the academic self-efficacy of nursing undergraduates, the generalized anxiety disorder scale-7, patient health questionnaire-9, academic self-efficacy scale, perceived social support scale and mindful attention awareness scale were conducted.ResultsModel fitness indexes of the structural equation model is good (CMIN/DF = 1.404, RMSEA = 0.042, GFI = 0.977, IFI = 0.977, TLI = 0.954, CFI = 0.975, NFI = 0.923). Structural equation model analysis showed that social support and mindfulness were the mediating variables of psychological distress on academic self-efficacy. Mediating variables accounted for 44% of the total effect value (− 0.3) with a value of − 0.132. Three paths were verified: psychological distress indirectly affected academic self-efficacy through social support (− 0.064); psychological distress indirectly affected academic self-efficacy through mindfulness (− 0.053); psychological distress indirectly affected academic self-efficacy through social support and mindfulness (− 0.015).ConclusionsSocial support and mindfulness play significant mediating roles in the effect of psychological distress on academic self-efficacy, and the chain mediating role of social support and mindfulness is also significant. Educators may mitigate the impact of psychological distress on academic self-efficacy by enhancing students’ social support and mindfulness.
Exploring the core competencies of clinical nurses in chinese tertiary hospitals: a qualitative content analysis
Authors: Chen Meihan , Wang Aiping , Zhou Baosen 
Published: May 2023

AbstractBackgroundWith the changes in social and medical environments and people’s health needs, the nursing core competency should be updated and developed promptly. This study aimed to explore the core competencies of nurses in Chinese tertiary hospitals under the new health development strategy.MethodsDescriptive qualitative research was conducted using qualitative content analysis. 20 clinical nurses and nursing managers from 11 different provinces and cities were interviewed via purposive sampling.ResultsData analysis revealed 27 competencies, which were grouped into three major categories according to the onion model. These categories were motivation and traits (responsibility, enterprise, etc.), professional philosophy and values (professionalism, career perception, etc.), and knowledge and skills (clinical nursing competency, leadership and management competency, etc.).ConclusionBased on the onion model, core competencies for nurses in Chinese tertiary hospitals were established, revealing three layers of core competencies and giving a theoretical reference for nursing managers to conduct competency training courses based on the competency levels.