bluuebunny/crossref_metadata_2025_split huggingface dataset:
Informing measurement of gender differences in suicide risk and resilience: A national study of United States military veterans
Authors: Smolenski Derek J. , McDonald Katie L. , Hoffmire Claire A. , Britton Peter C. , Carlson Kathleen F. , Dobscha Steven K. , Denneson Lauren M. 
Published: January 2023

AbstractObjectiveTo inform measure selection when examining gender differences in suicide risk, this paper evaluates measure performance for a set of gender‐relevant constructs and examines gender differences in mean scores.MethodsA national sample of veterans (n = 968) who had recently attempted suicide (past 6 months) completed measures assessing life experience‐, psychosocial‐, and health‐related constructs. A multigroup latent variable model was used to assess similarity of measurement properties between women and men.ResultsMetric and scalar invariance indicated that the latent variables functioned similarly between women and men. Women had higher scores on negative coping, institutional betrayal, and social rejection; men had higher scores on self‐compassion, autonomy, and suicide ideation.ConclusionsMeasurement properties and gender differences in mean values support the use of these measures for research on gender differences. Findings also suggest further investigation of social rejection, institutional betrayal, and negative coping among women veterans at risk for suicide.
Dysregulated cholesterol regulatory genes as a diagnostic biomarker for cancer
Authors: Kuldeep Seema , Soni Sneha , Srivastava Anubhav , Mishra Anjali , Sharma Lokendra Kumar , Mandal Chandi C. 
Published: January 2023

AbstractBackgroundA dysregulation of cholesterol homeostasis is often seen in various cancer cell types, and elevated cholesterol content and that of its metabolites appears to be crucial for cancer progression and metastasis. Cholesterol is a precursor of various steroid hormones and a key plasma membrane component especially in lipid‐rafts, also modulating many intracellular signaling pathways.MethodsTo provide an insight of dysregulated cholesterol regulatory genes, their transcript levels were analyzed in different cancers and their influence was correlated with the overall survival of cancer patients using cancer database analysis.ResultsThis analysis found a set of genes (e.g., ACAT1, RXRA, SOAT1 and SQLE) that were not only often dysregulated, but also had been associated with poorer overall survival in most cancer types. Quantitative reverse transcriptase‐polymerase chain reaction analysis revealed elevated SQLE and SOAT1 transcript levels and downregulated expression of RXRA and ACAT1 genes in triple negative breast cancer tissues compared to adjacent control tissues, indicating that this dysregulated expression of the gene signature is a diagnostic marker for breast cancer.ConclusionFor the first time, the present study identified a gene signature associated with the dysregulation of cholesterol homeostasis in cancer cells that may not only be used as a diagnostic marker, but also comprise a promising drug target for the advancement of cancer therapy.
Validation of a targeted metabolomics panel for improved second‐tier newborn screening
Authors: Mak Justin , Peng Gang , Le Anthony , Gandotra Neeru , Enns Gregory M. , Scharfe Curt , Cowan Tina M. 
Published: January 2023

AbstractImproved second‐tier assays are needed to reduce the number of false positives in newborn screening (NBS) for inherited metabolic disorders including those on the Recommended Uniform Screening Panel (RUSP). We developed an expanded metabolite panel for second‐tier testing of dried blood spot (DBS) samples from screen‐positive cases reported by the California NBS program, consisting of true‐ and false‐positives from four disorders: glutaric acidemia type I (GA1), methylmalonic acidemia (MMA), ornithine transcarbamylase deficiency (OTCD), and very long‐chain acyl‐CoA dehydrogenase deficiency (VLCADD). This panel was assembled from known disease markers and new features discovered by untargeted metabolomics and applied to second‐tier analysis of single DBS punches using liquid chromatography–tandem mass spectrometry (LC–MS/MS) in a 3‐min run. Additionally, we trained a Random Forest (RF) machine learning classifier to improve separation of true‐ and false positive cases. Targeted metabolomic analysis of 121 analytes from DBS extracts in combination with RF classification at a sensitivity of 100% reduced false positives for GA1 by 83%, MMA by 84%, OTCD by 100%, and VLCADD by 51%. This performance was driven by a combination of known disease markers (3‐hydroxyglutaric acid, methylmalonic acid, citrulline, and C14:1), other amino acids and acylcarnitines, and novel metabolites identified to be isobaric to several long‐chain acylcarnitine and hydroxy‐acylcarnitine species. These findings establish the effectiveness of this second‐tier test to improve screening for these four conditions and demonstrate the utility of supervised machine learning in reducing false‐positives for conditions lacking clearly discriminating markers, with future studies aimed at optimizing and expanding the panel to additional disease targets.
Systemic corticosteroids for the treatment of acute episodes of rhabdomyolysis in lipin‐1‐deficient patients
Authors: Tuchmann‐Durand Caroline , Roda Célina , Renard Perrine , Mortamet Guillaume , Bérat Claire‐Marine , Altenburger Lucile , de Larauz Marie Hug , Thevenet Eloise , Cottart Charles‐Henry , Moulin Florence , Bouchereau Juliette , Brassier Anais , Arnoux Jean‐Baptiste , Schiff Manuel , Bednarek Nathalie , Lamireau Delphine , Garros Alexa , Mention Karine , Cano Aline , Finger Lionel , Pelosi Michele , Brochet Cécile Sergent , Caccavelli Laure , Raphalen Jean‐Herlé , Renolleau Sylvain , Oualha Mehdi , de Lonlay Pascale 
Published: January 2023

AbstractMutations in the LPIN1 gene constitute a major cause of severe rhabdomyolysis (RM). The TLR9 activation prompted us to treat patients with corticosteroids in acute conditions. In patients with LPIN1 mutations, RM and at‐risk situations that can trigger RM have been treated in a uniform manner. Since 2015, these patients have also received intravenous corticosteroids. We retrospectively compared data on hospital stays by corticosteroid‐treated patients vs. patients not treated with corticosteroids. Nineteen patients were hospitalized. The median number of admissions per patient was 21 overall and did not differ when comparing the 10 corticosteroid‐treated patients with the 9 patients not treated with corticosteroids. Four patients in the non‐corticosteroid group died during a RM (mean age at death: 5.6 years). There were no deaths in the corticosteroid group. The two groups did not differ significantly in the number of RM episodes. However, for the six patients who had RM and occasionally been treated with corticosteroids, the median number of RM episodes was significantly lower when intravenous steroids had been administered. The peak plasma creatine kinase level and the area under the curve were or tended to be higher in patients treated with corticosteroids—even after the exclusion of deceased patients or focusing on the period after 2015. The median length of stay (10 days overall) was significantly longer for corticosteroid‐treated patients but was similar after the exclusion of deceased patients. The absence of deaths and the higher severity of RM observed among corticosteroid‐treated patients could suggest that corticotherapy is associated with greater survival.
Molecular advances in mycoremediation of polycyclic aromatic hydrocarbons: Exploring fungal bacterial interactions
Authors: Bokade Priyanka , Bajaj Abhay 
Published: January 2023

AbstractPolycyclic aromatic hydrocarbons (PAHs) are ubiquitous high global concern environmental pollutants and tend to bioaccumulate due to hydrophobic properties. These xenobiotics, having variable concentrations along different matrices, gradually undergo various physical, chemical, and biological transformation processes. Myco‐remediation aids accelerated degradation by effectively transforming complex ring structures to oxidized/hydroxylated intermediates, which can further funnel to bacterial degradation pathways. Exploitation of such complementing fungal−bacterial enzymatic activity can overcome certain limitations of incomplete bioremediation process. Furthermore, high‐throughput molecular methods can be employed to unveil community structure, taxon abundance, coexisting community interactions, and metabolic pathways under stressed conditions. The present review critically discusses the role of different fungal phyla in PAHs biotransformation and application of fungal−bacterial cocultures for enhanced mineralization. Moreover, recent advances in bioassays for PAH residue detection, monitoring, developing xenobiotics stress‐tolerant strains, and application of fungal catabolic enzymes are highlighted. Application of next‐generation sequencing methods to reveal complex ecological networks based on microbial community interactions and data analysis bias in performing such studies is further discussed in detail. Conclusively, the review underscores the application of mixed‐culture approach by critically highlighting in situ fungal−bacterial community nexus and its role in complete mineralization of PAHs for the management of contaminated sites.
Differential effects of rhizobacteria from uninfected and infected tomato on Meloidogyne incognita under protected cultivation
Authors: Gowda Manjunatha T. , Prasanna Radha , Kundu Aditi , Rana Virendra Singh , Rao Uma , Chawla Gautam 
Published: January 2023

AbstractIntermingled uninfected and root‐knot nematode‐infected tomato plants are commonly observed under protected cultivation. To understand the role of rhizobacteria underlying the susceptibility to nematode infectivity in these tomato plants, 36 rhizobacteria (18 from each type) with morphologically distinct colony characteristics were isolated from the rhizosphere of uninfected and root‐knot nematode‐infected tomato plants. The in vitro nematicidal potential of rhizobacteria from the uninfected rhizosphere was significantly higher than that from the infested rhizosphere. The three most effective antagonists were identified as Microbacterium laevaniformans, Staphylococcus kloosii, Priestia aryabhattai from root‐knot‐nematode‐infected tomato rhizosphere and Staphylococcus sciuri, Bacillus pumilus, and Priestia megaterium from the rhizosphere of uninfected tomato. Volatile organic compounds from these rhizobacteria were characterized. Except for S. kloosi, the soil drenching with other rhizobacteria significantly reduced juvenile penetration (>60%) in tomato roots. Furthermore, the application of a single or consortium of these rhizobacteria affected nematode reproduction in tomato. Four consortia of rhizobacteria (S. sciuri + B. pumilus + P. megaterium), (B. pumilus + P. megaterium), (S. sciuri + B. pumilus), and (S. sciuri + P. megaterium) from uninfested rhizosphere and two consortia (M. laevaniformans + P. aryabhattai), (M. laevaniformans + S. kloosii + P. aryabhattai) from infested rhizosphere (IRh) effectively reduced M. incognita reproduction and considerably enhanced plant growth and yield in tomato. The nematicidal efficacy, however, decreased when S. kloosii was applied in the consortium. These distinctive effects illustrate how the plant susceptibility to nematode infectivity is modulated under natural conditions.
Inflammation and micronutrient deficiency as major risk factors for anemia in children with intestinal failure: A longitudinal cohort study
Authors: Leite Heitor P. , Hatanaka Eduardo F. , Galati Sabio Giovana S. , Carvalho de Camargo Maria F. 
Published: January 2023

AbstractBackgroundMost data on anemia in children with intestinal failure (IF) have been obtained from studies in which the data were collected at a single point in time. We aimed to identify the frequency of anemia and factors associated with hemoglobin levels in children with IF during their course of home parenteral nutrition.MethodsWe performed a longitudinal cohort study of patients with IF followed up at a pediatric intestinal rehabilitation center. Outcome variables were hemoglobin levels and prevalence of anemia during the follow‐up period. The exposure variables were age, duration of parenteral nutrition, chronic disease, and serum concentrations of C‐reactive protein, iron, copper, selenium, vitamins A, D, B12, and folic acid.ResultsTwenty‐five children with a median time of receiving parenteral nutrition of 40.7 months were included. A median (and interquartile range) of 40.7 (25.2–58) hemoglobin measurements were performed per patient. Mean (SD) hemoglobin was 10.7 (1.8) g/dL at baseline and 11.6 (0.9) g/dL in the last observation (paired t test, P = 0.07); 32% of patients had mean hemoglobin values below the lower limit for age. In a multivariable predictive model, having C‐reactive protein >1 mg/dL was associated with a decrease of 0.57 g/dL in hemoglobin (95% CI, −0.90 to −0.24, P = 0.01), and an increase of 1 mg/L in vitamin A concentration was associated with the increase of 0.93 g/dL in Hb level (95% CI, 0.24–1.61; P = 0.008).ConclusionAnemia affects almost one‐third of children with IF and its frequency decreases during the follow‐up period. Hemoglobin levels are associated with inflammatory response and serum micronutrient concentrations.
Impact of percutaneous endoscopic gastrostomy on pediatric bone marrow transplantation outcomes: Retrospectice single‐center cohort study
Authors: Kairiene Igne , Vaisvilas Mantas , Vasciunaite Agnija , Tubutyte Geiste , Nedzelskiene Irena , Pasauliene Ramune , Muleviciene Audrone , Rascon Jelena 
Published: January 2023

AbstractBackgroundParenteral nutrition is commonly used to ensure nutrition support and prevent the harmful effects of malnutrition, which frequently occurs after allogeneic hematopoietic stem cell transplantation (aHSCT). Nevertheless, enteral nutrition supports the restoration of the gut barrier and microbiome as well as protects against infectious complications and acute graft‐vs‐host disease. Percutaneous endoscopic gastrostomy (PEG) may also be beneficial for gastric decompression and drug administration.MethodsWe performed a retrospective cohort study to evaluate the impact of PEG on treatment outcomes in 75 children who underwent aHSCT with (n = 34) or without (n = 41) PEG from 2005 to 2016.ResultsIn 34 patients, PEG was used to ensure enteral nutrition support (n = 30), oral drug intake (n = 28), and abdominal decompression (n = 2). During the study period, we observed a beneficial association between PEG placement and transplant‐related mortality as well as 5‐year overall survival compared with the non‐PEG group (12.9% vs 59.0%, P = 0.000; 85.3% vs 35.1%, P = 0.000, respectively). The beneficial impact of PEG was most prominent on 5‐year overall survival in older children (12–17 years) with grafts from matched unrelated donors.ConclusionsPEG placement had a positive association with transplant outcomes in pediatric patients undergoing aHSCT. To confirm these results, larger prospective studies are needed.
Extraction, identification, and starch‐digestion inhibition of phenolics from Euryale ferox seed coat
Authors: Zhang Li , Zeng Jiacheng , Yuan En , Chen Jiguang , Zhang Qingfeng , Wang Zongde , Yin Zhongping 
Published: January 2023

AbstractBACKGROUNDEuryale ferox is an important cash crop and valuable tonic in traditional medicine. The seeds of E. ferox are rich in starch, which is hard to digest, and the digestion speed is significantly slower than that of rice starch. The goal of this study was to evaluate the effects of E. ferox seed‐coat phenolics (EFCPs) on the digestion of E. ferox seed starch.RESULTSEFCPs were extracted and identified by ultra‐high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry. We optimized the extraction parameters, and the final extraction yield was about 1.49%. We identified seven phenolics from the E. ferox seed‐coat extracts: gallic acid, digalloylhexoside, catechin, procyanidin B2, epicatechin, ellagic acid, and epicatechin gallate. Quantitative analysis results showed that the E. ferox seed phenolics mainly distributed in the seed coat and the gallic acid, digalloylhexoside, and epicatechin gallate were three main phenolic compounds. The phenolics displayed strong inhibitory activities on α‐glucosidase and α‐amylase with an IC50 of 3.25 μg mL−1 and 1.36 mg mL−1 respectively. Furthermore, these phenolics could interact with starch by hydrogen bonds, which might make its starch more difficult to digest.CONCLUSIONOur investigation suggests that the EFCPs can strongly inhibit the digestion of E. ferox seed starch by inhibiting the α‐amylase and α‐glucosidase activities and interacting with starch by hydrogen bonds; therefore, E. ferox seeds have a promising application prospect in foods for hypoglycemia. © 2023 Society of Chemical Industry.
Direct chiral separation of abscisic acid by high‐performance liquid chromatography with a phenyl column and a mobile phase containing γ‐cyclodextrin
Authors: Terashima Hiroyuki , Mutoh Yui , Aizawa Sen‐ichi , Taga Atsushi , Mikami Ikko , Itabashi Yutaka , Tsutsumiuchi Kaname , Yamamoto Atsushi , Kodama Shuji 
Published: January 2023

Abscisic acid (2‐cis,4‐trans‐abscisic acid) is a plant hormone that has an asymmetric carbon atom. We tried to separate the enantiomers of native abscisic acid by HPLC using a phenyl column and a chiral mobile phase containing γ‐cyclodextrin. The optimum mobile phase conditions were found to be 0.8% (w/v) γ‐cyclodextrin, 4% (v/v) acetonitrile, and 20 mM phosphate buffer (pH 6.0). It was found that (R)‐abscisic acid was earlier detected than (S)‐abscisic acid. Since γ‐cyclodextrin is hardly retained on a phenyl column, it was suggested that (R)‐abscisic acid formed a more stable complex with γ‐cyclodextrin than the (S)‐abscisic acid. Abscisic acid in an acacia honey sample was successfully enantioseparated with the proposed method and only (S)‐abscisic acid was detected. A biologically inactive 2‐trans,4‐trans‐abscisic acid, which was prepared by irradiation of abscisic acid with a light‐emitting diode lamp at 365 nm, was partially enantioseparated by the proposed method. Since the irradiation of (S)‐abscisic acid‐induced cis‐to‐trans isomerization to produce one 2‐trans,4‐trans‐abscisic acid enantiomer, it is reasonable that racemization did not proceed during the cis‐to‐trans isomerization. (S)‐Abscisic acid and probably (S)‐2‐trans,4‐trans‐abscisic acid were detected in a honey sample, where the peak area of (S)‐abscisic acid was 7 times larger than that of (S)‐2‐trans,4‐trans‐abscisic acid.
Primary amide‐functionalized cyclotricatechylene covalent organic frameworks membrane for efficient enrichment of melamine and its derivatives in migration solution of food contact materials
Authors: Lin Jiana , Ouyang Xiaoyan , Hu Yuling , Li Gongke , Zhong Qisheng 
Published: January 2023

A highly chemically stable primary amide‐functionalized cyclotricatechylene covalent organic framework was synthesized by an irreversible reaction and a post‐synthetic modification. It possessed a rod‐like morphology and exhibited strong solvent stability owing to the polyether bonds. The material showed good adsorption performance for melamine and its derivatives and adsorption mechanism was investigated by molecular simulations. The adsorbent was coated on the nylon‐66 membrane to prepare the enrichment membrane. Under optimized conditions, an in‐syringe membrane‐based extraction method, combined with ultra‐high performance liquid chromatography‐tandem mass spectrometry was developed for the analysis of melamine and six melamine derivatives in the migration solution. A good linearity was obtained with correlation coefficients ranging from 0.9924 to 0.9995. The limits of detection were 1–200 ng/L and the limits of quantification were 3–500 ng/L. This method was successfully applied to the migration solution of sushi bamboo rolling mats with spiked recoveries of 73.2%–115% and relative standard deviations of 0.9%–9.9%. This work shows a practical and perspective approach for the efficient enrichment of food contact material hazards.
The Incidence of Diaphragmatic Dysfunction in Patients Presenting With Dyspnea in the Emergency Department
Authors: Puchongmart Chanokporn , Nakornchai Tanyaporn , Leethotsarat Kewalin , Monsomboon Apichaya , Prapruetkit Nattakarn , Ruangsomboon Onlak , Riyapan Sattha , Surabenjawong Usapan , Chakorn Tipa 
Published: January 2023

ObjectivesDiaphragmatic dysfunction has been reported as a cause of dyspnea, and its diagnosis can be made using ultrasound. Diaphragmatic ultrasound is mainly used to predict respiratory failure in chronic conditions. The use of diaphragmatic ultrasound has also risen in acute settings, such as emergency departments (EDs). However, the number of studies on its use still needs to be increased. The present study aimed to find the incidence of diaphragmatic dysfunction in the ED.MethodsThis prospective cohort study was conducted in an ED. We enrolled patients aged greater than 18 years who presented with dyspnea. Diaphragmatic excursion and diaphragmatic thickness techniques were performed. The primary outcome was the incidence of diaphragmatic dysfunction. The secondary outcomes were the associations between diaphragmatic dysfunction and the composition of respiratory therapies within 24 hours, intubation within 24 hours, and 7‐day mortality.ResultsA total of 237 patients were analyzed. The incidences of diaphragmatic dysfunction assessed by diaphragmatic excursion and diaphragm thickness were 22.4 and 32.1%, respectively. Patients with sepsis and cancer had the highest incidences. Diaphragmatic dysfunction assessed by both techniques was not associated with the composition of respiratory support therapies within 24 hours, intubation within 24 hours, or 7‐day mortality.ConclusionsThe incidence of diaphragmatic dysfunction in dyspneic patients in the ED ranged from 22.4 to 32.1%, depending on the ultrasound technique. Diaphragmatic dysfunction was not associated with the composition of respiratory support therapies, intubation, or mortality.
Performance investment decision and pricing strategy for platforms considering two‐sided users' different preferences
Authors: Zhang Zhuoning , Hua Zhongsheng 
Published: January 2023

AbstractThis paper examines a hybrid strategy of pricing control and performance investment for two‐sided platforms. While many studies have investigated performance improvement in two‐sided markets, few studies have noticed that users may have different investment preferences. We build a strategic model to explore how platforms can make a trade‐off between investing in high performance to satisfy customer preferences versus reducing investment to facilitate service provider participation. We provide insights on the optimum investment strategy and the corresponding charging or subsidizing controls and demonstrate how ignoring the opposite preferences can lead to errors in investing in platform performance.