bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robust circulating microRNA signature for the diagnosis and early detection of pancreatobiliary cancer
Authors: Mitsunaga Shuichi , Ikeda Masafumi , Ueno Makoto , Kobayshi Satoshi , Tsuda Masahiro , Miki Ikuya , Kuwahara Takamichi , Hara Kazuo , Takayama Yukiko , Matsunaga Yutaro , Hanada Keiji , Shimizu Akinori , Yoshida Hitoshi , Nomoto Tomohiro , Takahashi Kenji , Iwamoto Hidetaka , Iwama Hideaki , Hatano Etsuro , Nakata Kohei , Nakamura Masafumi , Sudo Hiroko , Takizawa Satoko , Ochiai Atsushi 
Published: January 2025

Abstract

Background
A new circulating biomarker superior to carbohydrate antigen 19–9 (CA19-9) is needed for diagnosing pancreatobiliary cancer (PBca). The aim of this study was to identify serum microRNA (miRNA) signatures comprising reproducible and disease-related miRNAs.

Methods
This multicenter study involved patients with treatment-naïve PBca and healthy participants. The optimized serum processing conditions were evaluated using t-distributed stochastic neighbor embedding (t-SNE) visualization. Serum miRNA candidates for disease association were selected using weighted gene coexpression network analysis (WGCNA). A miRNA signature combining multiple serum miRNAs was tested in exploratory, validation, and independent validation sets. The synthesis and secretion of diagnostic miRNAs were evaluated using human pancreatic cancer cells.

Results
In total, 284 (150 healthy and 134 PBca) of 827 serum samples were processed within 2 h of blood collection before freezing, distributed in the same area as that in the t-SNE map, and assigned to an exploratory set. The 193 optimized samples were assigned to either the validation (50 healthy, 47 PBca) or independent validation (50 healthy, 46 PBca) set. Index-1, a combination of five serum miRNAs (hsa-miR-1343-5p, hsa-miR-4632-5p, hsa-miR-4665-5p, hsa-miR-665, and hsa-miR-6803-5p) with disease association in WGCNA, showed a sensitivity and specificity of > 80% and an AUC outperforming that of CA19-9 in the exploratory, validation, and independent validation sets. The AUC of Index-1 was superior to that of CA19-9 (0.856 vs. 0.649, p = 0.038) for detecting T1 tumors. miR-665, a component of Index-1, was expressed in human pancreatic cancer cells, and its transfection inhibited cell growth.

Conclusions
The serum miRNA signature Index-1 is useful for detecting PBca and could facilitate the early diagnosis of PBca. These findings can help improve clinical PBca detection by providing an optimized biomarker that overcomes the limitations of the current standard.
First autochthonous case of Opisthorchis felineus in Austria
Authors: Kulmer Lisa-Maria , Unterköfler Maria Sophia , Vali Yasamin , Schwendenwein Ilse , Luckschander-Zeller Nicole 
Published: January 2025

Abstract

Background

Opisthorchis felineus is a feline pathogen with zoonotic potential that can be a causative agent of human opisthorchiasis and cholangiocarcinoma. In Europe, O. felineus is particularly endemic in Eastern European countries, while this parasite has also been sporadically detected in Germany, Italy and northern Poland. Parts of Asia, such as Russia, Ukraine and Kazakhstan, are also affected.

Methods
A 7-year-old female neutered European Shorthair cat, without any traveling history, presented in May 2023 with weight loss, anorexia and vomiting.

Results
The cat showed increased liver enzyme activities, hyperbilirubinemia and hyperammonemia consistent with the suspected diagnosis of cholangitis with consecutive hepatoencephalopathy. Eggs of O. felineus were detected by routine cytological examination of bile smears and PCR confirmed O. felineus.

Conclusions
This is the first report of autochthonous O. felineus infection in Austria.

Graphical Abstract
Impact of sodium alginate hydrogel containing bacteriophage peptides that specifically bind to the EtCab protein on the inhibition of Eimeria tenella infection
Authors: Chen Hang , Zhi Wenjing , Bai Bingrong , Anjum Faisal R. , Jia Zhipeng , Kong Rui , Liu Qiuju , Wang Biao , Ma Chunli , Ma Dexing 
Published: January 2025

Abstract
Avian coccidiosis, caused by the protozoan Eimeria, leads to significant economic losses for the poultry industry. In this study, bacteriophages that specifically bind to the calcium-binding protein (EtCab) of Eimeria tenella were selected using a biopanning process with a pIII phage display library. The recombinant EtCab protein served as the ligand in this selection process. The binding ability of target phages to the EtCab protein or E. tenella sporozoites was evaluated. The role of peptides corresponding to target phages in inhibiting the invasion of E. tenella sporozoites into cells was analysed using flow cytometry. Subsequently, the phages were encapsulated in sodium alginate to protect them from degradation in gastric fluid, which has a low pH value. Chickens were orally administered both microencapsulated and non-microencapsulated phages, and the protective effects against E. tenella infection were assessed. The binding mechanism of these peptides to the EtCab protein was investigated through in silico analysis. The results indicated that three specific phages (Y, G, and V) could bind effectively to recombinant EtCab protein as well as to sporozoite proteins. All three peptides, particularly Y and G, demonstrated significant inhibition of sporozoite invasion into cells in vitro. Additionally, oral administration of the encapsulated phages Y and G provided a higher level of protection against Eimeria infection compared to encapsulated phage V and the unencapsulated phages. Molecular docking studies revealed that three peptides, particularly Y and G, efficiently bind to the EtCab protein through hydrogen bonds. This study provides a reference for developing small molecular drugs targeting coccidiosis.
Effects of gestational diabetes mellitus and diabetes mellitus on lipid profile, antioxidants, hormones and electrolytes status in a population of Nigerian women
Authors: Akinjiyan Moses Orimoloye , Atiba Adeniran Samuel , Asaolu Modupe Fisayo 
Published: January 2025

Abstract

Aim
Gestational diabetes mellitus (GDM) cases are rising globally. This research examined the effects of GDM and diabetes mellitus whose hallmark is hyperglycemia on hormones, lipid profiles, electrolytes and antioxidants in freshly diagnosed women attending teaching hospitals in Ekiti State, Nigeria.

Methods
The cross-sectional design followed convenience sampling in four groups (n = 50): gestational diabetes women (GDM), normal pregnant women (NP), diabetes nonpregnant women (DM) and nondiabetes nonpregnant women (ND-NP). Blood samples were collected from fasting subjects through antecubital fossa. BMI, FPG, TC, TG, LDL-C, HDL-C, sodium, potassium and bicarbonate ions, MDA and antioxidants were investigated using standard procedures and spectrophotometer. Results were analysed using one-way ANOVA and p < 0.05 was used as significant difference.

Results
There was significant (p < 0.05) increase in FPG of GDM and DM (> 5.56 mmol/l) subjects compared to NP and ND-NP subjects. TC, TG, HDL-C and LDL-C were elevated in GDM and DM groups compared to NP and ND-NP groups. Sodium ion concentration was significantly (p˂0.05) elevated in ND-NP group relative to GDM and DM groups. There was significant (p < 0.05) increase in MDA levels in DM and NP groups compared to ND-NP. Superoxide dismutase activity was significantly (p < 0.05) greater in ND-NP than in DM and NP. Progesterone level was greater in GDM group than in other groups. The FSH concentration was lower in the GDM and NP groups than in the DM and ND-NP groups, and prolactin concentration was greater in the GDM group than in the NP.

Conclusion
This study suggested that GDM and DM could result in hormonal and electrolyte imbalances, elevated lipid profiles, and reduced antioxidant levels.
Development and application of sugarcane yellow leaf virus vectors in sugarcane
Authors: Yin Yuteng , Zhao Pingzhi , Sun Yanwei , Han Tian , Wang Mengrong , Yan Meixin , Wu Jianming , Zhou Hui , Ye Jian 
Published: January 2025

Abstract
The aphid-transmitted polerovirus, sugarcane yellow leaf virus (SCYLV), is one of the most widespread sugarcane viruses and causes huge economic losses to the global sugarcane production. Molecular studies and disease resistance breeding against SCYLV are impeded both by the lack of infectious clones and comprehensive knowledge of this virus. In this study, we present the development of the first infectious cDNA clone of SCYLV, which could efficiently infect Nicotiana benthamiana, Arabidopsis thaliana and sugarcane plants. SCYLV-based recombinant heterologous EGFP protein vector was also developed. Using this system, we identified the green peach aphid, Myzus persicae, as a new compatible transmission vector and recognized LC05-136 and GT10-2118 as more resistant cultivars of SCYLV than Badila. This system provides an indispensable resource for both SCYLV biological research and sugarcane breeding efforts.
Clinical Manifestations and Treatment Challenges in Infants and Children With Classic Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
Authors: Nokoff Natalie J , Buchanan Cindy , Barker Jennifer M 
Published: January 2025

Abstract
The most common form of congenital adrenal hyperplasia (CAH) is secondary to 21-hydroxylase deficiency (21OHD). This review will summarize the clinical manifestations, recommended treatments, monitoring, clinical challenges and management strategy, and treatment challenges in special situations for infants and children with classic CAH due to 21OHD. Specifically, we review newborn screening and the initial diagnosis, glucocorticoid and mineralocorticoid treatment, and recommended monitoring, including anthropometric and laboratory measures. Children with CAH may have premature adrenarche, precocious puberty, and early growth plate closure and have an increased risk of hypertension and overweight/obesity. Many 46,XX individuals will also have genital differences, which may include clitoromegaly and/or a urogenital sinus. We review psychosocial and surgical considerations, including suggestions on how to talk with children, family, and caregivers about bodily difference. These suggestions may be used by families and/or providers caring for individuals with CAH.
Measurements of Iodination in Thyroglobulin: A Step Toward the Next Generation of Thyroid Cancer Monitoring
Authors: Maus Anthony , Thompson Chris , Grebe Stefan K G 
Published: January 2025

Abstract
Thyroglobulin (Tg) is a 330-kDa homodimeric protein that that is the prohormone of thyroid hormones triiodothyronine (T3) and thyroxine (T4). The most critical steps of thyroid hormone synthesis by Tg are iodination and fusion of specific tyrosine residues that are in close proximity to each other in the folded Tg protein. The degree of Tg iodination has been studied widely to determine if it is correlated with thyroid autoimmune disease with mixed results, but these efforts have been limited by the lack of an effective quantitative technique. Simultaneously, the treatment of thyroid cancer has undergone a shift toward partial thyroidectomies, thus undermining the value of Tg measurements. A possible alternative to established monitoring techniques is measurement of Tg iodination states as it has been shown that tumor-derived Tg has significantly lower iodine content. Such measurements require a thorough understanding of normal iodination status. In this study, state-of-the-art liquid chromatography–tandem mass spectrometry (LC-MS/MS) instrumentation is used to perform bottom-up proteomics experiments and identify iodinated residues within commercially available Tg. Using this technique, sequence coverages greater than 90% were achieved, which resulted in identification of previously identified and novel hormone synthesis and donor sites. Based on the results of these discovery experiments, 5 iodination sites were selected for targeted quantitative LC-MS/MS measurements, which suggested that hormone synthesis occurs predominantly at Y24 and Y2766. The results presented herein lay the foundation for routine measurements of iodinated residues, which has the potential to overcome the limitations of current monitoring techniques and benefit patient care.
Acute mountain sickness: pathophysiology and prevention
Authors: Moczydłowski Paweł , Kuczapska Karolina , Gliwa Anna , Ryglewicz Monika , Fabian Dariusz , Leszczyńska-Knaga Elżbieta , Jakubczyk Natalia , Rutkowska-Kawalec Weronika , Kurowski Marek , Michalczuk Karolina 
Published: January 2025

This review discusses the epidemiology, pathophysiology, prevention and treatment of acute mountain sickness (AMS). Key mechanisms including changes in blood-brain barrier permeability, activation of the renin-angiotensin-aldosterone system and the role of diuresis in the development of AMS are analysed. The effectiveness of various preventive strategies, such as acclimatisation and pharmacotherapy (acetazolamide, dexamethasone, ibuprofen), as well as alternative approaches, including the use of natural remedies such as Rhodiola roses, coca leaves and Ginkgo biloba, are discussed. The paper emphasises the importance of correct diagnosis and prompt action in the treatment of AMS, which is crucial in preventing complications.

Material and methods
Data bases such as Pubmed and GoogleScholar were used for research with the key words: acute mountain illness (AMS), acetazolamid, dexametazone, altitude, prevention, acclimatisation.

Conclusions
Acute altitude sickness is a challenge in terms of understanding pathophyslogy and effective treatment methods. The primary methods of preventing AMS are properly managed acclimatisation and the use of acetazolamide. Other pharmacological and natural measures require further research.
Cystic Fibrosis and Reproductive Outcomes: The Latest Insights into Fertility and Pregnancy
Authors: Małagocka Weronika , Zagórski Karol , Kozik Mateusz , Skalska-Dziobek Nina , Chybowska Karolina , Naruszewicz Maria , Cetnarowski Przemysław 
Published: January 2025

Introduction and purpose: Due to improvement in treatment of people with cystic fibrosis (CF), the number of adult patients increases. CF patients are living longer, with better quality of life and a growing number of them are considering parenthood. Most people with CF are suffering because of reduced fertility, however the number of pregnant CF patients rises. The aim of this paper was to summarize the date available in the literature and recent reports about the fertility, pregnancy and treatment of pregnant CF patients.

Material and methods: The literature was reviewed in PubMed database, GoogleScholar and in European Cystic Fibrosis Society database with the use of keywords.

State of knowledge:78% of women with CF declare that they want to have a child in the future. Reduced fertility may be overcome with assisted reproduction technologies, which also gives an opportunity to do preimplantation genetic tests. CFTR modulators therapy during the pregnancy was described in a small number of studies, but the current experts statement declares that those drugs are probably safe for the infant, and discontinuing this therapy is connected with an increased risk of pulmonary exacerbation in mothers.

Summary: Women with CF nowadays have an opportunity to become parents. Despite fertility lower than in the general population, they are able to become pregnant. The decision about the conception should be planned before and consulted with the CF team. Planning the pregnancy gives time to prepare the mother, optimize her nutrition status and make sure medications she takes are safe for the fetus. Data about the use of CFTR modulators during pregnancy and breastfeeding are limited. In the future the MAYFLOWER study may determine the impact of CFTR modulators on pregnancy and breastfeeding.
A Preregistered Falsification Test of the Decision by Sampling Model and Rank-Order Effect
Authors: Forsgren Mattias , Frimanson Lars , Juslin Peter 
Published: January 2025

Many social scientists have assumed that people’s preferences can be described by stable and coherent “utility” functions. This notion of stable utility functions has been challenged by cognitive psychologists who suggest that preferences are malleable and constructed in the moment, but neither camp has explained how the subjective valuations underpinning preferences arise. One influential attempt to do so is the Decision by Sampling (DbS) model, which suggests that a quantitative attribute’s (e.g., money sum’s) subjective value is its rank order in a momentarily activated memory sample. DbS thus implies that manipulating the recently experienced attribute distribution should change people’s subsequent valuations of that attribute: for example, from the typically assumed concave shape of the utility function to a convex shape. However, recent studies have pointed out methodological concerns in the evidence previously thought to support this prediction (and thus, DbS). In this preregistered study, we replicate the previous paradigm but address the methodological concerns to test if such a “rank-order” manipulation does change valuations. We derive qualitative predictions from DbS to verify that our conditions yield distinct predictions. We find strong evidence against the DbS’s prediction that a “rank-order” manipulation changes what options the participants select and how strongly they prefer the options. We also find extreme evidence in favor of a contextualization effect, implying that people value formally identical gambles differently depending on whether they cue a real-life setting or not. Although we encourage replication by independent laboratories, these results suggest that the DbS is falsified for this binary choice task. This paper was accepted by Yuval Rottenstreich, behavioral economics and decision analysis. Funding: This research was funded by the Marcus and Amalia Wallenberg Foundation [Grant MAW 2016.0132] and the Swedish Research School of Management and IT. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2022.03611 .
Pyramidal hyperbolic metamaterials fabricated using an anodic alumina template
Authors: Huang Jingchao , Sun Hong , Geng Lulu , Pan Yinuo , Wang Wenyan , Wen Rong , Ji Ting , Cui Yanxia , Li Guohui 
Published: January 2025

The manipulation of light at the nanoscale is of great significance in engineering electromagnetic spaces and controlling light propagation. Stratified hyperbolic metamaterials (HMMs), with their highly anisotropic properties, can be further patterned to achieve precise control over light, enabling a wide range of applications in advanced optical technologies such as energy harvesting, sensing, and near-field light manipulation. Reported patterned HMM devices, primarily fabricated using electron beam lithography and etching processes, are costly and unsuitable for large-area manufacturing. In this paper, we report the fabrication of well-aligned, large-area HMM nanoarrays composed of alternating layers of thermally evaporated Ag and MoO3 on a glass substrate. This was achieved using a cost-effective, structurally tunable, ultrathin anodic aluminum oxide (AAO) template. Our approach enables the formation of an HMM structure with a pyramidal geometry, which demonstrates a significant reduction in reflection, decreasing to below 25% within the visible light spectrum (375–685 nm). Theoretical analysis suggests that the observed anti-reflection effect in this wavelength range is attributed to the excitation of plasmonic resonances. This method for fabricating patterned HMM structures can be applied to various substrates, offering a low-cost solution for HMM device production. The resulting HMM devices hold potential for use in advanced optical systems, such as customized optical absorbers and enhanced photodetection instruments.
Effectiveness of app-based cognitive behavioral therapy for insomnia on preventing major depressive disorder in youth with insomnia and subclinical depression: A randomized clinical trial
Authors: Chen Si-Jing , Que Jian-Yu , Chan Ngan Yin , Shi Le , Li Shirley Xin , Chan Joey Wing Yan , Huang Weizhen , Chen Chris Xie , Tsang Chi Ching , Ho Yuen Lam , Morin Charles M. , Zhang Ji-Hui , Lu Lin , Wing Yun Kwok 
Published: January 2025

Background
Increasing evidence suggests that insomnia plays an important role in the development of depression, supporting insomnia intervention as a promising approach to prevent depression in youth. This randomized controlled trial evaluated the effectiveness of app-based cognitive behavioral therapy for insomnia (CBT-I) in preventing future onset of major depressive disorder (MDD) in youth.

Methods and findings
This was a randomized, assessor-blind, parallel group-controlled trial in Chinese youth (aged 15−25 years) with insomnia disorder and subclinical depressive symptoms. Participants were randomly assigned (1:1) to 6-week app-based CBT-I or 6-week app-based health education (HE) delivered through smartphones. Online assessments and telephone clinical interviews were conducted at baseline, post-intervention, 6- and 12-month follow-ups. The primary outcome was time to onset of MDD. The secondary outcomes included depressive symptoms and insomnia at both symptom and disorder levels. Between September 9, 2019, and November 25, 2022, 708 participants (407 females [57%]; mean age, 22.1 years [SD = 1.9]) were randomly allocated to app-based CBT-I group (n = 354) or app-based HE group (n = 354). Thirty-seven participants (10%) in the intervention group and 62 participants (18%) in the control group developed new-onset MDD throughout the 12-month follow-up, with a hazard ratio of 0.58 (95% confidence interval 0.38–0.87; p = 0.008). The number needed to treat to prevent MDD at 1 year was 10.9 (6.8–26.6). The app-based CBT-I group has higher remission rates of insomnia disorder than the controls at post-intervention (52% versus 28%; relative risk 1.83 [1.49–2.24]; p < 0.001) and throughout 12-month follow-up. In addition, the CBT-I group reported a greater decrease in depressive (adjusted difference −1.0 [−1.6 to −0.5]; Cohen’s d = 0.53; p < 0.001) and insomnia symptoms (−2.0 [−2.7 to −1.3], d = 0.78; p < 0.001) than the controls at post-intervention and throughout 6-month follow-up. Insomnia was a mediator of intervention effects on depression. No adverse events related to the interventions were reported.

Conclusions
App-based CBT-I is effective in preventing future onset of major depression and improving insomnia outcomes among youth with insomnia and subclinical depression. These findings highlight the importance of targeting insomnia to prevent the onset of MDD and emphasize the need for wider dissemination of digital CBT-I to promote sleep and mental health in the youth population.

Trial registration
ClinicalTrials.Gov (NCT04069247).