bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aspirin 15cH has Different Effects on Morphology and Function of Lipopolysaccharide-Challenged RAW 264.7 Macrophages In Vitro Compared to a Pharmacological Dose of Aspirin
Authors: von Ancken Adalberto C. , de Medeiros Nathalia Salles S. , Perdomo Sandra Kalil , Cruz Mario Costa , Alvares-Saraiva Anuska M. , Perez Elizabeth C. , Silva Rodrigo Augusto da , Eizayaga Francisco Xavier , Bonamin Leoni Villano 
Published: July 2023

Abstract
Introduction Aspirin is one of the most commonly used drugs worldwide. It is known to present antipyretic, anti-inflammatory and anti-thrombotic actions, making it extremely useful in a wide range of clinical contexts. Interestingly, homeopathically prepared Aspirin 15cH has been found to have a pro-thrombotic effect in rats, raising the hypothesis that Aspirin 15cH could also modulate the activity of inflammatory cells in different pathological processes.
Objective Our objective was to assess what effect Aspirin 15cH has on RAW 264.7 macrophages in vitro.
Methods The effects of Aspirin 15cH on biochemical and morphological activities of lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages were evaluated. These effects were compared with unchallenged macrophages (negative control), untreated LPS-stimulated macrophages, macrophages treated with succussed water (vehicle control), or aspirin 200 µg/mL (pharmacological inhibitor of LPS activity). Cell morphology (adhered cell area and cytoskeleton arrangements), cell viability, toll-like receptor-4 (TLR-4) expression, and the production of nitric oxide, cytokines and intracellular reactive oxygen species were assessed.
Results Aspirin 15cH reduced the number of cells expressing TLR-4 on the surface (p = 0.03) and induced a “columnar” morphology of macrophage pseudopods, indicating changes in cytoskeleton arrangement. When cells were treated with both Aspirin 15cH and LPS, cell morphology became heterogeneous, suggesting that sub-populations of cells had differing sensitivities to LPS or Aspirin 15cH. Exposure of the cells to LPS alone, succussed water or aspirin 200 µg/mL produced effects consistent with the literature.
Conclusion Aspirin 15cH, aspirin 200 µg/mL, LPS and succussed water appear to act as independent stimuli able to induce different patterns of macrophage response. Aspirin 15cH induced changes suggestive of M2 polarization of the macrophages (i.e., toward a wound healing or tissue repair, rather than inflammatory, phenotype). These preliminary findings need to be confirmed in further specific studies.
Platelet Function Decreases with Increasing Severity of Liver Cirrhosis and Portal Hypertension—A Prospective Study
Authors: Brusilovskaya Ksenia , Hofer Benedikt Silvester , Simbrunner Benedikt , Eichelberger Beate , Lee Silvia , Bauer David J. M. , Mandorfer Mattias , Schwabl Philipp , Panzer Simon , Reiberger Thomas , Gremmel Thomas 
Published: July 2023

Background Cirrhotic patients display an increased risk for both bleeding and thrombosis. We investigated platelet activation across Child–Pugh stages (CPSs) and portal hypertension (PH) severity.
Material and Methods A total of 110 cirrhotic patients were prospectively included. CPS and hepatic venous pressure gradient (HVPG) were determined. Platelet surface expression of P-selectin and activated glycoprotein (GP) IIb/IIIa were measured by flow cytometry before/after stimulation with protease-activated receptor (PAR)-1 (thrombin receptor activating peptide, TRAP) and PAR-4 (AYPGKF) agonists, epinephrine, and lipopolysaccharide (LPS).
Results Platelet count was similar across CPS but lower with increasing PH severity. Expression of P-selectin and activated GPIIb/IIIa in response to TRAP and AYPGKF was significantly reduced in platelets of CPS-B/C versus CPS-A patients (all p < 0.05). Platelet P-selectin expression upon epinephrine and LPS stimulation was reduced in CPS-C patients, while activated GPIIb/IIIa in response to these agonists was lower in CPS-B/C (all p < 0.05). Regarding PH severity, P-selectin and activated GPIIb/IIIa in response to AYPGKF were lower in HVPG ≥20 mmHg patients (both p < 0.001 vs. HVPG < 10 mmHg). Similarly, activated GPIIb/IIIa was lower in HVPG ≥20 mmHg patients after TRAP stimulation (p < 0.01 vs. HVPG < 10 mmHg). The lower platelet surface expression of P-selectin and activated GPIIb/IIIa upon stimulation of thrombin receptors (PAR-1/PAR-4) in CPS-B/C and HVPG ≥20 mmHg patients was paralleled by reduced antithrombin-III levels in those patients (all p < 0.05). Overall, PAR-1- and PAR-4-mediated platelet activation correlated with antithrombin-III levels (p < 0.001).
Conclusion Platelet responsiveness decreases with increasing severity of liver cirrhosis and PH but is potentially counterbalanced by lower antithrombin-III levels.
Endovascular and Medical Management of Unruptured Intracranial Aneurysms
Authors: Reddy Aravind , Masoud Hesham E. 
Published: July 2023

AbstractUnruptured intracranial aneurysms are often discovered incidentally on noninvasive imaging. As use of noninvasive imaging has increased, our understanding of the presumed prevalence of intracranial aneurysms in adults has increased. Incidentally found aneurysms are often asymptomatic; however, they can rarely rupture and cause life-threatening illness. Elective treatment of intracranial aneurysms carries risks which need to be considered along with patient-specific factors (e.g., anatomy, medical comorbidities, personal preferences). In this article, we review the natural history, risk factors for cerebral aneurysm formation and rupture, evidence for medical management, and the safety profile and efficacy of available endovascular treatment options.
Remote Robotic Surgery and Virtual Education Platforms: How Advanced Surgical Technologies Can Increase Access to Surgical Care in Resource-Limited Settings
Authors: Sherif Youmna A. , Adam Mohammed A. , Imana Aimee , Erdene Sarnai , Davis Rachel W. 
Published: July 2023

AbstractAdvanced surgical technologies consist of remote and virtual platforms that facilitate surgical care and education. It also includes the infrastructure necessary to utilize these platforms (e.g., internet access, robotic systems, and simulators). Given that 5 billion people lack access to safe and timely surgical care, the appeal of these technologies to the field of global surgery lies primarily in its ability to eliminate geographical barriers and address surgeon shortages. This article discusses the use of virtual and remote technologies in resource-limited settings, the potential applications of these technologies, the possible barriers to their integration, and the impact these technologies may have on access to surgical care and education. Specifically, it will explore how robotic surgery, telesurgery, virtual education platforms, and simulations have the potential to be instrumental in enhancing worldwide access to safe surgical care.
A Giant Coronary Aneurysm in a Suspected Kawasaki Disease Causing Asymptomatic Myocardial Ischemia
Authors: Mondal Sudipta , Walse Rohit , Pant Bhagwati Prasad , Abhilash Sreevilasam P. , Sasidharan Bijulal 
Published: July 2023

AbstractCoronary involvement in Kawasaki disease is not uncommon; however, giant coronary aneurysm exceeding 50 mm is extremely rare. In this article, we presented a case of giant coronary aneurysm involving right coronary artery with associated asymptomatic myocardial ischemia as evident by multimodality imaging.
Establishment of the first Australian public and health-professional palliative care advice service: exploring caller needs and gaps in care
Authors: Marco David J. T. , McMillan-Drendel Esther , Philip Jennifer A. M. , Williamson Theresa , Le Brian 
Published: July 2023

This study explores and describes the state-wide needs of the first 1000 calls to the newly established Victorian Palliative Care Advice Service (PCAS). A retrospective analysis investigated calls from the Victorian general public (n = 618 calls) and healthcare professionals (n = 382 calls) to PCAS between 26 May 2020 and 24 October 2022. Caller demographics, disease type, reason for call, and perceived utility of service were described. Most calls were from members of the public (62%) and related to malignant conditions (41%). Regional/rural clients comprised 45% of all calls to the service, of which half (50%) were health professionals seeking advice on symptom management and medication. One-third (29.3%) of all calls from health professionals were escalated to a palliative care medical consultant. PCAS prevented calls to emergency services in 10% of cases, and 82% of callers reported their issue was ‘very much’ or ‘completely’ addressed by PCAS. PCAS was shown to be frequently used by the public and healthcare professionals supporting patients with advanced, life-limiting illnesses. The service provided a solution without requiring complex technology, delivering a rapid connection for consumers with specialist palliative care expertise that might otherwise be unavailable, particularly in regional areas.
Energy intake and nutritional balance of roe deer (Capreolus capreolus) in special Bavarian landscapes in southern Germany
Authors: König Andreas , Dahl Sarah-Alica , Windisch Wilhelm 
Published: July 2023

Context Irrespective of the fact that the European roe deer (Capreolus capreolus) occurs from the Mediterranean to north of the Arctic Circle and is one of the most abundant and widespread species, roe deer are fed in winter in Germany and Austria. Hunters justify the supplementary feeding with the argument that roe deer do not find sufficient high-quality food in our landscape and they would have to starve in winter. Aims Our aim was to measure the quality and energy content of the browsed roe deer diet (in terms of metabolisable energy, ME) and the daily energy intake by roe deer. Methodology Between 2017 and 2019, rumenreticula of 629 roe deer were collected from five extreme habitats in Bavaria. Samples were examined by standard methods of dietary analysis, such as in vitro ruminal fermentation, crude nutrient analysis and the analysis of neutral/acid detergent fibre and acid detergent lignin. In addition, data on body condition and age were collected for each roe deer. Key results The diet consumed by roe deer has an energy density between 5.1 MJ/kg dry matter (DM) and 6.1 MJ/kg DM on average. Crude fibre contents in the diet varied between 20% and 38% DM. Roe deer compensate for lower energy densities in vegetation by consuming more diet. Across all habitats, adult consumed an annual mean of between 10.6 and 12.9 MJ ME/day. Conclusions Roe deer find sufficient high-energy food in all landscape types. They consume and can utilise raw fibre to the same extent as do red deer or mouflon. Differences in energy density among habitats result from carbohydrate content and are compensated for by more food intake. Energy deficits in roe deer could not be detected in any habitat at any time. Implications Roe deer do not need supplementary feeding in any habitat. Supplementary feeding leads to browsing damage because of the lack of raw fibre in the feed.
Diet and impacts of non-native fallow deer (Dama dama) on pastoral properties during severe drought
Authors: Davis Naomi E. , Forsyth David M. , Bengsen Andrew J. 
Published: July 2023

Context There is concern about potential competition between non-native fallow deer (Dama dama) and livestock for food, particularly during times of low rainfall when pasture is scarce. Aims We aimed to estimate the extent to which a high-density fallow deer population (~37 deer per km2) competed with livestock for food during a severe drought on pastoral properties on the Liverpool Plains, New South Wales, Australia. Methods We collected rumen contents from 125 fallow deer shot from helicopters during control operations in June and August 2018, and used microhistology to quantify their diets. We then used the diet data to adjust published estimates of stock unit equivalence from farmed fallow deer. Fallow deer sex and age class abundances and stock unit equivalences were multiplied to estimate the grazing pressure of the fallow deer population pre- and post-control relative to recommended sheep and cattle stocking rates. Finally, we estimated density–impact relationships for fallow deer. Key results Fallow deer diet was dominated by monocots (primarily grasses), but some individuals consumed large amounts of dicots (commonly Eucalyptus). Fallow deer were grazers to intermediate mixed feeders. Fallow deer dry-sheep equivalents (DSEs, based on a 50-kg merino wether) varied from 1.01 (juveniles) to 1.85 (adult females and adult males). Assuming complete diet overlap, the pre-control fallow deer population was equivalent to approximately 60.3 DSEs per km2 and reduced the potential stocking rate of domestic livestock by 50.2%. Discounting the fallow deer DSEs by the amount of browse in the diet resulted in a pre-control population of 45.7 DSEs per km2. The helicopter-based shooting reduced the fallow deer population by 26%, increasing the stocking rate in natural pasture by 22.0% (complete overlap) or 13.8% (discounting for browse). Conclusions When food is limiting, fallow deer at high density can exert strong competitive pressure on livestock, reducing carrying capacity. Browsing, including on Eucalyptus, likely helped fallow deer to persist at high densities during the severe drought of 2018, when properties had destocked. Implications Helicopter-based shooting reduced the competitive effects of fallow deer on livestock in our study area to only a small extent, but more intensive shooting would have led to a greater reduction. The helicopter-based shooting of deer was received positively by landholders at a time of severe stress, suggesting that deer control in agricultural areas can have other important benefits.
Twelve weeks of concurrent resistance and interval training improves military occupational task performance in men and women
Authors: Sterczala Adam J. , Krajewski Kellen T. , Peterson Patrick A. , Sekel Nicole M. , Lovalekar Mita , Wardle Sophie L. , O’Leary Thomas J. , Greeves Julie P. , Flanagan Shawn D. , Connaboy Christopher , Nindl Bradley C. 
Published: July 2023

ABSTRACTIn the British Army, ground close combat roles have opened to women, however, they must pass the newly developed, gender‐neutral Role Fitness Tests for Soldiers (RFT(S)). Due to physiological differences between sexes, training that optimally prepares both sexes for military occupational demands and the RFT(S) is needed. The purpose of this study was to determine the efficacy of a 12‐week periodized strength and power programme with concurrent interval training on RFT(S) performance and determine if performance adaptations differed between sexes. 39 recruit‐aged (18–35 yrs) participants, including 21 men (29 ± 1 yrs) and 18 women (27 ± 1 yrs), completed the study. Participants performed 3 training sessions per week that included strength and power resistance training followed by interval training. Pre‐ to post‐training, improvements were observed for seated medicine ball throw (4.5%, p < 0.001), casualty drag (29.8%, p < 0.001), single lift (8.9%, p < 0.001), water can carry (13.8%, p = 0.012), repeated lift and carry (6.5%, p < 0.001), 2‐km load carriage (7.2%, p < 0.001) and 2‐km run (3.2%, p = 0.021). Pre‐ to post‐training improvements were also observed for maximal squat (27.0%, p < 0.001), bench press (8.9%, p < 0.001) and deadlift (24.6%, p < 0.001) maximal strength, but not upper body power or aerobic capacity. No differences in RFT(S) improvements were observed between sexes, however men performed better than women in all RFT(S) and physical performance measures. Concurrent resistance and interval training improves military occupational performance in men and women; however, women may need more training than men to pass the gender‐neutral RFT(S).
Exploring the natural history of intrinsic capacity impairments: longitudinal patterns in the 10/66 study
Authors: Gonzalez-Bautista Emmanuel , Llibre-Guerra Jorge Jesus , Sosa Ana L , Acosta Isaac , Andrieu Sandrine , Acosta Daisy , Llibre-Rodríguez Juan de Jesús , Prina Matthew 
Published: July 2023

Abstract

Background
intrinsic capacity (IC) is a construct encompassing people’s physical and mental abilities. There is an implicit link amongst IC domains: cognition, locomotion, nutrition, sensory and psychological. However, little is known about the integration of the domains.

Objectives
to investigate patterns in the presentation and evolution of IC domain impairments in low-and-middle-income countries and if such patterns were associated with adverse outcomes.

Methods
secondary analyses of the first two waves of the 10/66 study (population-based surveys conducted in eight urban and four rural catchment areas in Cuba, Dominican Republic, Puerto Rico, Venezuela, Peru, Mexico and China). We applied latent transition analysis on IC to find latent statuses (latent clusters) of IC domain impairments. We evaluated the longitudinal association of the latent statuses with the risk of frailty, disability and mortality, and tested concurrent and predictive validity.

Results
amongst 14,923 participants included, the four latent statuses were: high IC (43%), low deterioration with impaired locomotion (17%), high deterioration without cognitive impairment (22%), and high deterioration with cognitive impairment (18%). A total of 61% of the participants worsened over time, 35% were stable, and 3% improved to a healthier status.
Participants with deteriorated IC had a significantly higher risk of frailty, disability and dementia than people with high IC. There was strong concurrent and predictive validity. (Mortality Hazard Ratio = 4.60, 95%CI 4.16; 5.09; Harrel’s C = 0.73 (95%CI 0.72;0.74)).

Conclusions
half of the study population had high IC at baseline, and most participants followed a worsening trend. Four qualitatively different IC statuses or statuses were characterised by low and high levels of deterioration associated with their risk of disability and frailty. Locomotion and cognition impairments showed other trends than psychological and nutrition domains across the latent statuses.
FGCNSurv: dually fused graph convolutional network for multi-omics survival prediction
Authors: Wen Gang , Li Limin 
Published: July 2023

AbstractMotivationSurvival analysis is an important tool for modeling time-to-event data, e.g. to predict the survival time of patient after a cancer diagnosis or a certain treatment. While deep neural networks work well in standard prediction tasks, it is still unclear how to best utilize these deep models in survival analysis due to the difficulty of modeling right censored data, especially for multi-omics data. Although existing methods have shown the advantage of multi-omics integration in survival prediction, it remains challenging to extract complementary information from different omics and improve the prediction accuracy.ResultsIn this work, we propose a novel multi-omics deep survival prediction approach by dually fused graph convolutional network (GCN) named FGCNSurv. Our FGCNSurv is a complete generative model from multi-omics data to survival outcome of patients, including feature fusion by a factorized bilinear model, graph fusion of multiple graphs, higher-level feature extraction by GCN and survival prediction by a Cox proportional hazard model. The factorized bilinear model enables to capture cross-omics features and quantify complex relations from multi-omics data. By fusing single-omics features and the cross-omics features, and simultaneously fusing multiple graphs from different omics, GCN with the generated dually fused graph could capture higher-level features for computing the survival loss in the Cox-PH model. Comprehensive experimental results on real-world datasets with gene expression and microRNA expression data show that the proposed FGCNSurv method outperforms existing survival prediction methods, and imply its ability to extract complementary information for survival prediction from multi-omics data.Availability and implementationThe codes are freely available at https://github.com/LiminLi-xjtu/FGCNSurv.
LncRNA-encoded peptides: unveiling their significance in cardiovascular physiology and pathology—current research insights
Authors: Zhang Li , Tang Mi , Diao Haoyang , Xiong Liling , Yang Xiao , Xing Shasha 
Published: July 2023

Abstract
Long non-coding RNAs (lncRNAs), which are RNA transcripts exceeding 200 nucleotides were believed to lack any protein-coding capacity. But advancements in -omics technology have revealed that some lncRNAs have small open reading frames (sORFs) that can be translated by ribosomes to encode peptides, some of which have important biological functions. These encoded peptides subserve important biological functions by interacting with their targets to modulate transcriptional or signalling axes, thereby enhancing or suppressing cardiovascular disease (CVD) occurrence and progression. In this review, we summarize what is known about the research strategy of lncRNA-encoded peptides, mainly comprising predictive websites/tools and experimental methods that have been widely used for prediction, identification, and validation. More importantly, we have compiled a list of lncRNA- encoded peptides, with a focus on those that play significant roles in cardiovascular physiology and pathology, including ENSRNOT (RNO)-sORF6/RNO-sORF7/RNO-sORF8, dwarf open reading frame (DOWRF), myoregulin (NLN), etc. Additionally, we have outlined the functions and mechanisms of these peptides in cardiovascular physiology and pathology, such as cardiomyocyte hypertrophy, myocardial contraction, myocardial infarction, and vascular remodelling. Finally, an overview of the existing challenges and potential future developments in the realm of lncRNA-encoded peptides was provided, with consideration given to prospective avenues for further research. Given that many lncRNA-encoded peptides have not been functionally annotated yet, their application in CVD diagnosis and treatment still requires further research.