bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complications of the Prone Transpsoas Lateral Lumbar Interbody Fusion for Degenerative Lumbar Spine Disease: A Multicenter Study
Authors: Soliman Mohamed A. R. , Diaz-Aguilar Luis , Kuo Cathleen C. , Aguirre Alexander O. , Khan Asham , San Miguel-Ruiz Jose E. , Amaral Rodrigo , Abd-El-Barr Muhammad M. , Moss Isaac L. , Smith Tyler , Deol Gurvinder S. , Ehresman Jeff , Battista Madison , Lee Bryan S. , McMains M. Craig , Joseph Samuel A. , Schwartz David , Nguyen Andrew D. , Taylor William R. , Pimenta Luiz , Pollina John 
Published: June 2023

BACKGROUND AND OBJECTIVES:
The prone transpsoas (PTP) approach for lateral lumbar interbody fusion (LLIF) is a novel technique for degenerative lumbar spine disease. However, there is a paucity of information in the literature on the complications of this procedure, with all published data consisting of small samples. We aimed to report the intraoperative and postoperative complications of PTP in the largest study to date.

METHODS:
A retrospective electronic medical record review was conducted at 11 centers to identify consecutive patients who underwent LLIF through the PTP approach between January 1, 2021, and December 31, 2021. The following data were collected: intraoperative characteristics (operative time, estimated blood loss [EBL], intraoperative complications [anterior longitudinal ligament (ALL) rupture, cage subsidence, vascular and visceral injuries]), postoperative complications, and hospital stay.

RESULTS:
A total of 365 patients were included in the study. Among these patients, 2.2% had ALL rupture, 0.3% had cage subsidence, 0.3% had a vascular injury, 0.3% had a ureteric injury, and no other visceral injuries were reported. Mean operative time was 226.2 ± 147.9 minutes. Mean EBL was 138.4 ± 215.6 mL. Mean hospital stay was 2.7 ± 2.2 days. Postoperative complications included new sensory symptoms—8.2%, new lower extremity weakness—5.8%, wound infection—1.4%, cage subsidence—0.8%, psoas hematoma—0.5%, small bowel obstruction and ischemia—0.3%, and 90-day readmission—1.9%.

CONCLUSION:
In this multicenter case series, the PTP approach was well tolerated and associated with a satisfactory safety profile.
The generation and characterisation of anti-insect blood cell monoclonal antibodies
Authors: Mullett H. , Ratcliffe N. A. , Rowley A. F. 
Published: June 2023

ABSTRACT
A number of monoclonal antibodies have been raised against the haemocytes (blood cells) of two insect species: namely, the wax moth, Galleria mellonella and a species of cockroach, Blaberus discoidalis. In the case of the wax moth, two groups of monoclonal antibodies were raised. One group, designated GM1 to GM28, identified only one type of blood cell, namely the gran-ular cell. All 28 monoclonal antibodies recognised a 90 kDa protein as shown by western blotting. The second group of monoclonal antibodies, designated GallPG1 to GallPG20, recognised all the haemocyte types of G. mel -lonella, including the granular cells, prohaemocytes, plasmatocytes, spherule cells and oenocytoids. These latter monoclonal antibodies all identified a protein of 35 kDa when tested by western blotting. All monoclonal antibodies against G. mellonella haemocytes were highly specific for wax moth haemocytes and did not cross-react with any other lepidopteran, orthopteran or dic-tyopteran haemocytes tested. With the cockroach, B. discoidalis, there were two main sets of monoclonal anti-bodies produced against the haemocytes; however, none was specific for any of the three haemocyte types found in this species, namely, the granular cells, plasmatocytes and prohaemocytes. One group of Blaberus monoclonal antibodies, termed BlabMem1 to BlabMem19, labelled the cell surface and cytoplasm of the plasmatocytes, pro-haemocytes and granular cells. These monoclonal anti-bodies all recognised a protein of approximately 60 kDa. The second group of monoclonal antibodies, designated BlabGr1 to BlabGr31, labelled the granular inclusions of all haemocyte types. The monoclonal antibodies against the Blaberus haemocytes did cross-react with the blood cells of some closely related species of cockroach, but not with the haemocytes of any of the lepidopterans or orthopterans tested. Initial experiments showed that exposure of Galleria haemocytes to the monoclonal anti-bodies specific for the 90 kDa protein (GM1 to GM28), significantly reduced the ability of these cells to attach to bacteria in vitro. These results suggest that this pro-tein plays a central role in the cellular defence reactions of insects and that these monoclonal antibodies should be extremely useful in further investigating its func-tional significance.
Localisation of Nramp1 in macrophages: Modulation with activation and infection
Authors: Searle Susan , Bright Nicholas A. , Roach Tamara I. A. , Atkinson Peter G. P. , Barton C. Howard , Meloen Robert H. , Blackwell Jenefer M. 
Published: June 2023

ABSTRACTThe murine natural resistance-associated macrophage protein, Nramp1, has multiple pleiotropic effects on macrophage activation and regulates survival of intracellular pathogens including Leishmania, Salmonella and Mycobacterium species. Nramp1 acts as an iron transporter, but precisely how this relates to macrophage activation and/or pathogen survival remains unclear. To gain insight into function, anti-Nramp1 monoclonal and polyclonal antibodies are used here to localise Nramp1 following activation and infection. Confocal microscope analysis in uninfected macrophages demonstrates that both the mutant (infection-susceptible) and wild-type (infection-resistant) forms of the protein localise to the membranes of intracellular vesicular compartments. Gold labelling and electron microscopy defines these compartments more precisely as electron-lucent late endosomal and electrondense lysosomal compartments, with Nramp1 colocalizing with Lamp1 and cathepsins D and L in both compartments, with macrosialin in late endosomes, and with BSA-5 nm gold in pre-loaded lysosomes. Nramp1 is upregulated with interferon-γ and lipopolysaccaride treatment, coinciding with an increase in labelling in lysosomes relative to late endosomes and apparent dispersion of Nramp1-positive vesicles from a perinuclear location towards the periphery of the cytoplasm along the microtubular network. In both control and activated macrophages, expression of the protein is 3-to 4-fold higher in wild-type compared to mutant macrophages. In Leishmania major-infected macrophages, Nramp1 is observed in the membrane of the pathogen-containing phagosomes, which retain a perinuclear localization in resting macrophages. In Mycobacterium avium-infected resting and activated macrophages, Nramp1-positive vesicles migrated to converge, but not always fuse, with pathogen-containing phagosomes. The Nramp1 protein is thus located where it can have a direct influence on phagosome fusion and the microenvironment of the pathogen, as well as in the more general regulation of endosomal/lysosomal function in macrophages.
Identification of cofilin, coronin, Rac and capZ in actin tails using a Listeria affinity approach
Authors: David Violaine , Gouin Edith , Troys Marleen Van , Grogan Ann , Segal Anthony W. , Ampe Christophe , Cossart Pascale 
Published: June 2023

ABSTRACT
Actin assembly is involved in cell motility and intracellular movement of Listeria monocytogenes. Induction of Listeria actin tails is mediated by the surface protein ActA. The N-terminal domain of ActA is sufficient for this function. Cell components known to play a role in the actin-based motility of Listeria are VASP (vasodilatator-stimulated phosphoprotein), the multiprotein Arp2/3 complex and cofilin. VASP interacts with the central domain of ActA. Proteins interacting with the N-terminal domain of ActA have not been identified. To identify novel host cell components of ActA-induced actin tails, we used bovine brain extracts and an affinity approach with Listeria as matrix. Several known components of Listeria tails were isolated including VASP, Arp3 and cofilin. Cofilin was identified by peptide sequencing, and cofilin recruitment and Listeria tail length were found to be pH-dependent, in agreement with its recently reported role in enhancing actin filament turnover. In addition, three proteins not previously known to be associated with Listeria tails, coronin, Rac and capZ, were identified in our affinity approach. In infected cells, the localization of the identified proteins was studied by immunofluorescence. Our findings suggest that these latter proteins, which are known to play critical roles in cellular actin rearrangements, may also be involved in the dynamics of Listeria-induced actin assembly.
The IQGAP-related protein DGAP1 interacts with Rac and is involved in the modulation of the F-actin cytoskeleton and control of cell motility
Authors: Faix Jan , Clougherty Chris , Konzok Angelika , Mintert Ursula , Murphy John , Albrecht Richard , Mühlbauer Bettina , Kuhlmann Jürgen 
Published: June 2023

ABSTRACT
DGAP1 of Dictyostelium discoideum is a cell cortex associated 95 kDa protein that shows homology to both RasGTPase-activating proteins (RasGAPs) and RasGAP-related proteins. When tested for RasGAP activity, recombinant DGAP1 protein did not promote the GTPase activity of human H-Ras or of Dictyostelium RasG in vitro. Instead, DGAP1 bound to Dictyostelium Rac1A and human Rac1, but not to human Cdc42. DGAP1 preferentially interacted with the activated GTP-bound forms of Rac1 and Rac1A, but did not affect the GTPase activities. Since Rho-type GTPases are implicated in the formation of specific F-actin structures and in the control of cell morphology, the microfilament system of mutants that either lack or overexpress DGAP1 has been analysed. DGAP1-null mutants showed elevated levels of F-actin that was organised in large leading edges, membrane ruffles or numerous large filopods. Expression of actin fused to green fluorescent protein (GFP) was used to monitor the actin dynamics in these cells, and revealed that the F-actin cytoskeleton of DGAP1-null cells was rapidly re-arranged to form ruffles and filopods. Conversely, in DGAP1-overexpressing cells, the formation of cellular projections containing F-actin was largely suppressed. Measurement of cell migration demonstrated that DGAP1 expression is inversely correlated with the speed of cell motility.
A cell cycle regulated MAP kinase with a possible role in cytokinesis in tobacco cells
Authors: Calderini Ornella , Bögre László , Vicente Oscar , Binarova Pavla , Heberle-Bors Erwin , Wilson Cathal 
Published: June 2023

ABSTRACT
Mitogen-activated protein (MAP) kinases have been demonstrated to have a role in meiosis but their involvement in mitotic events is less clear. Using a peptide antibody raised against the tobacco MAP kinase p43Ntf6 and extracts from synchronized tobacco cell suspension cultures, we show that this kinase is activated specifically during mitosis. Entry into mitosis appears to be necessary for the activation of the kinase, which occurs as a post-translational event. The activation of the kinase occurs in late anaphase/early telophase. The p43Ntf6protein shows a transient localization to the cell plate in anaphase cells, in the middle of the two microtubule arrays characteristic of the phragmoplast, a plant-specific structure involved in laying down the new cell wall. The combined data support a role for the MAP kinase p43Ntf6 in cytokinesis in tobacco cells.
OSMANLI DEVRİ İSTANBUL TASVİRLERİNDE KIZ KULESİ BETİMLEMELERİ
Authors: ÖTEN Aliye 
Published: June 2023

Öz
Restorasyon nedeniyle özlediğimiz şu günlerde Kız Kulesi’ni Osmanlı kitaplarında ve yapılarında aramak, her biri farklı ellerin ve bakışların izini taşıyan birbirinden oldukça farklı İstanbul tasvirlerini karşımıza çıkarmıştır. Osmanlı coğrafyasına yayılan geniş örneklem alanında yüzyıllar içinde yapılan bu nakışlardaki Kız Kulesi figürleri; tasarım, teknik ve gerçeklik açısından incelenmiştir. Bu figürler topografik kent tasvirleri, kalem işi ve duvar resimlerinde İstanbul tasvirleri başlıkları altında ayrı ayrı incelenerek kronolojik çerçevede meydana gelen değişim ve gelişim özellikleri tespit edilmeye çalışılmıştır. İnsan elinin değdiği hayalle gerçeğin birbirine karıştığı her sanat eserinde olduğu gibi güzellik hissini oluşturan o karışımı anlatma gibi zorlama bir çabadan ziyade ilham kaynağı olan ve İstanbul gibi bir şehrin simge yapısı Kız Kulesi’ni sanat gerçekliği ve güzelliğiyle sunmak ana hedefi teşkil etmektedir.

Anahtar kelimeler: Osmanlı, kalem işi, İstanbul tasvirleri, kız kulesi

Abstract
A contemporary look at the Maiden's Tower in Ottoman books and buildings, which we miss due to the restoration, revealed quite different depictions of Istanbul, each of which bore traces of several hands and eyes. This study examined the Maiden's Tower depictions in those embroideries, which had a long history and spread over a broad region in the Ottoman Empire. The figures were assessed in terms of design, technique, and reality and categorized under the headings of topographic urban depictions, hand-drawn illustrations, and wall paintings of Istanbul. The chronological changes in those figures were also described in this endeavor. Just like any attempt to describe an artwork's imaginary and realistic aspects, our primary intention was to depict the Maiden's Tower, a unique and inspirational symbol of Istanbul, with its artistic reality and beauty.

Keywords: Ottoman, hand-drawn artwork, Istanbul depictions, Maiden's Tower
Floristic composition and carbon stock estimation under restored mangrove area in Bagan Serdang, North Sumatra, Indonesia
Authors: HAREFA MELINDA SURIANI , NASUTION ZULKIFLI , TUHONO ELING , SUSILOWATI ARIDA 
Published: June 2023

Abstract. Harefa MS, Nasution Z, Tuhono E, Susilowati A. 2023. Floristic composition and carbon stock estimation under restored mangrove area in Bagan Serdang, North Sumatra, Indonesia. Biodiversitas 24: 2037-2044. Mangrove forests play an important role in the surrounding ecosystem, providing various complex ecosystem services such as coastal protection, food and medicine provision, water quality improvement, and habitat for various terrestrial and aquatic biota. However, forest degradation and conversion have destroyed the majority of mangrove habitats. Monoculture restoration of mangroves with local species has been carried out in various areas, including Bagan Serdang village in North Sumatra, to restore the degraded area. However, fewer studies have been conducted to assess the floristic diversity of monoculture restoration areas and their carbon stock storage estimation, especially in North Sumatra. This research aims to analyze vegetation diversity and estimate carbon stocks in a restoration area dominated by Avicennia marina (Forssk.) Vierh. species in Bagan Serdang village, North Sumatra, Indonesia. Purposive sampling was employed to identify species and measure tree diameter at breast height by creating plot lines that filled the observation area with 10 x 10 plots. Therefore, the Shannon Wiener diversity index (H') and allometric equations were used to determine mangrove diversity and biomass. The study's findings revealed that A. marina had the highest Importance Value Index (IVI) at all growth stages, including seedlings, saplings, and trees, with values of 89.12, 190.91, and 263.14%, respectively. The seedling stages had the highest diversity, with a value of 1.241. The total amount of CO2 absorbed is 200.67 tons/ha, and the total amount of carbon stored is 54.67MgC H-1.
Ethnobotanical study on using bamboo for kites making in Sumedang District, West Java, Indonesia
Authors: IHSAN MUHAMMAD , IRAWAN BUDI , ISKANDAR BUDIAWATI SUPANGKAT , ISKANDAR JOHAN 
Published: June 2023

Abstract. Ihsan M, Irawan B, Iskandar BS, Iskandar J. 2023. Ethnobotanical study on using bamboo for kites making in Sumedang District, West Java, Indonesia. Biodiversitas 24: 2393-2401. Bamboo has diverse socio-economic, cultural, and ecological roles in rural areas. Residentsin Cijambu Village, Tajungsari District, Sumedang Regency, West Java, usually use bamboo for kite crafts based on local or Traditional Ecological Knowledge (TEK). However, currently, the craft activities of the residents of Cijambu Village in making kites tend to decline. This is due to the reduced kite demand, and the younger generation is less interested in kite-making crafts. As a result, it is difficult for the kite-making industry in Cijambu Village to provide jobs and creates an opportunityfor the villagers to maintain its sustainability. In addition, the decline in the kite-making industry could cause the gradual erosion and subsequent loss of the TEK. Therefore, this study aims to elucidate the TEK of Cijambu Village on bamboo species and landraces, the utilization of bamboo for the kite-making industry, and its benefits for rural communities. The method used in this study was qualitative with an ethnobotanical approach; there are 12 informants comprising seven men and five women between the ages of 35 and 70 years involved in this study. The resultof the study showed thata total of 5 landraces and species of bamboo, i.e.: awi tali (Gigantochloa apus (Schult.f.) Kurz), awi bitung (Dendrocalamus asper (Schult.f.) Backerex K. Heyne), awi surat (Gigantochloa verticillata (Willd.) Munro), awi gombong héjo (Gigantochloa verticillata (Willd.) Munro), awi temen (Gigantochloa atter (Hassk.) Kurz),and awi haur konéng (Bambusa vulgaris Schrad. ex J.C.Wendl. var. Striata (Lodd.ex Lindl.) Gamble) in Cijambu Village. The G. apus have predominantly been used for making kites, creating many job opportunities for men and women, and diversifying household income. However, the future sustainability of the kite industry in Cijambu Village tends to be very challenging because the demand for kites is declining occasionally. At the same time, the younger generation is less interested in kites making, and the TEK gradually erodes withinthe younger generation. Thus, it was revealed that the use of bamboo for the kite craft industry had formed biocultural systems, as well as changes in the culture and linguistics of villagers, which can affect the management and conservation of bamboo in rural areas.
Evidencing leprosy neuronal inflammation by 18-Fluoro-deoxy-glucose
Authors: Penna Patricia Sola , De Souza Sergio Augusto Lopes , De Lacerda Paulo Gustavo Limeira Nobre , Rodrigues Pitta Izabela Jardim , Spitz Clarissa Neves , Sales Anna Maria , Lara Flavio Alves , De Souza Ana Caroline Siquara , Sarno Euzenir Nunes , Pinheiro Roberta Olmo , Jardim Marcia Rodrigues 
Published: June 2023

Background
Leprosy is caused by multiple interactions between Mycobacterium leprae (M. leprae) and the host’s peripheral nerve cells. M. leprae primarily invades Schwann cells, causing nerve damage and consequent development of disabilities. Despite its long history, the pathophysiological mechanisms of nerve damage in the lepromatous pole of leprosy remain poorly understood. This study used the findings of 18F-FDG PET/CT on the peripheral nerves of eight lepromatous patients to evaluate the degree of glucose uptake by peripheral nerves and compared them with clinical, electrophysiological, and histopathological evaluations.

Methods
Eight patients with lepromatous leprosy were included in this study. Six patients were evaluated up to three months after leprosy diagnosis using neurological examination, nerve conduction study, 18F-FDG PET/CT, and nerve biopsy. Two others were evaluated during an episode of acute neuritis, with clinical, neurophysiological, and PET-CT examinations to compare the images with the first six.

Results
Initially, six patients already had signs of peripheral nerve injury, regardless of symptoms; however, they did not present with signs of neuritis, and there was little or no uptake of 18F-FDG in the clinically and electrophysiologically affected nerves. Two patients with signs of acute neuritis had 18F-FDG uptake in the affected nerves.

Conclusions
18F-FDG uptake correlates with clinical neuritis in lepromatous leprosy patients but not in silent neuritis patients. 18F-FDG PET-CT could be a useful tool to confirm neuritis, especially in cases that are difficult to diagnose, such as for the differential diagnosis between a new episode of neuritis and chronic neuropathy.
Adoption, Memory, and Cold War Greece: Kid Pro Quo?, Gonda Van Steen (2019)
Authors: Hantzaroula Pothiti 
Published: June 2023

Review of: Adoption, Memory, and Cold War Greece: Kid Pro Quo?, Gonda Van Steen (2019)
Ann Arbor, MI: University of Michigan Press, 323 pp.,
ISBN 978-0-47213-158-7, h/bk, $94.95
ISBN 978-0-47203-881-7, p/bk, $39.95

Zitountai paidia apo tin Ellada: Yiothesies stin Ameriki tou Psychrou Polemou, Gonda Van Steen (2021) (Trans. Ariadne Loukakou)
Athens: Potamos, 520 pp.,
ISBN 978-9-60545-173-8, p/bk, €25.20
Strategic reading comprehension in L2 and L3: assuming relative interdependence within Cummins’ linguistic interdependence hypothesis
Authors: Talebi Seyed Hassan , Fallahi Javad , Amjadi Ali 
Published: June 2023

Abstract
This experimental study attempts to put to the test Cummins’ (1978) Linguistic Interdependence Hypothesis (LIH) in reading between English (L2) and Arabic (L3). Two groups of comparison and experimental attended this study. The experimental group received reading strategy instruction while the comparison group received the traditional form of reading instruction in L2. Both groups received reading comprehension tests in L2 and L3 before and after the instructions. An interview was finally conducted for a more in-depth investigation. Analysis of quantitative data evinced overall reading comprehension ability significantly improved only in the experimental group in both languages, supporting Cummins’ LIH. Componentially, only the inferential component improved significantly for the experimental group, showing different components contribute differently to the interdependence between languages. Qualitative findings showed miscellaneous results in linguistic, cognitive, and affective domains which were evidence of both interdependence and non-interdependence between L2 and L3. The pedagogical implication is further discussed.
Crystal structure of tetracarbonyl-{μ-[N-(diphenylphosphanyl)-N,P,P-triphenylphosphinous amide]}-bis[μ-(phenylmethanethiolato)]diiron (Fe–Fe), C48H39Fe2NO4P2S2
Authors: Gao Xin-Ping , Lü Shuang , Li Qian-Li 
Published: June 2023

Abstract
C48H39Fe2NO4P2S2, orthorhombic, P21 (no. 4), a = 11.4073(11) Å, b = 16.9013(14) Å, c = 11.8261(12) Å, α = 90°, β = 108.297(4)°, γ = 90°, V = 2164.8(4) Å3, Z = 2, R
gt(F) = 0.0355, wR
ref(F
2) = 0.0740, T = 298(2) K.
Crystal structure of catena-[(μ3-bromido)-(1H-1,2,4-triazol-1-yl)benzoato-κ1
N)copper(I)], C9H7BrCuN3O2
Authors: Yuan Zhi-Min , Xia Yu-Pei 
Published: June 2023

Abstract
C9H7BrCuN3O2, triclinic, P1̄ (no. 2), a = 3.8736(3) Å, b = 7.5238(7) Å, c = 18.1555(19) Å, α = 84.581(1)°, β =

88.166

(
2
)

°

$88.166(2){}^{\circ}$

, γ = 81.551(1)°, V = 520.96(8) Å3, Z = 2, R
gt(F) = 0.0344, wR
ref(F
2) = 0.0845, T = 298(2) K.
Uncertainty quantification by probabilistic analysis of circular fins
Authors: Gorla Rama Subba Reddy 
Published: June 2023

Abstract
The temperature distribution and thermal Stresses induced by a temperature difference for steady state heat transfer in silicon carbide (SiC) ceramic tube heat exchanger with circular fins was computationally simulated by a finite element method and probabilistically evaluated in view of the several uncertainties in the performance parameters. Cumulative distribution functions and sensitivity factors were computed for the hoop stresses due to the structural and thermodynamic random variables. These results are used to identify the most critical design variables in order to optimize the design and make it cost effective. The probabilistic analysis leads to the selection of the appropriate measurements to be used in structural and heat transfer analysis and to the identification of both the most critical measurements and parameters.