bluuebunny/crossref_metadata_2025_split huggingface dataset:
P-526 Modified Applebaum uterine scoring system to predict uterine receptivity in frozen embryo transfer(FET) cycles
Authors: Sapkota A , Sharma S 
Published: June 2023

Abstract

Study question
To evaluate modified Applebaum uterine scoring system in predicting the uterine receptivity and clinical pregnancy rate?

Summary answer
The modified version of Applebaum uterine scoring system has sensitivity of 0.9411 and specificity of 0.5869 in predicting the uterine receptivity in embryo transfer cycle.

What is known already
The endometrial receptivity have been accepted to be major limiting factors in the establishment of pregnancy. Different techniques have been developed to evaluate endometrial receptivity, such as endometrial receptivity array or histological dating. The advantage of Ultrasound is it’s non-invasiveness, repeatability, real time monitoring and predictability.

Study design, size, duration
It is a prospective observational study. Study group comprises of 200 female with primary infertility over a period of 6 month.

Participants/materials, setting, methods
Exclusion criteria was patients associated with male sub-fertility or having uterine anomalies. The study was conducted at D.Y.Patil fertility centre, unit of Bloom IVF. All the patients were given uterine scoring before FET based on Endometrial thickness, morphology, vascularisation, myometrial echogenicity, uterine artery PI and EDF, myometrial blood flow.

Main results and the role of chance
The overall pregnancy rate was 54%. A perfect score of 20 was associated with 97.4% pregnancy rate. Scores of 17 to 19 was associated with the pregnancy rate of 85.4% and scores of 14 to 16 was associated with the pregnancy rate of 28.6%. Score less than 13 is not associated with any pregnancy. The Modified Applebaum scoring system showed the sensitivity of 0.9411 and specificity of 0.5869 in predicting the outcome of the cycle.
On an individual basis, endometrial morphology, end diastolic blood flow(EDF) and Pulsatility index(PI) of uterine artery and myometrial blood flow were found to be significant for a pregnancy on the basis of Z score and p value of &lt; 0.01

Limitations, reasons for caution
As there were no case of secondary infertility, Grade III/IV endometriosis, endocrine disorder and uterine anomalies. The scoring system might not replicate the same results.

Wider implications of the findings
As the sensitivity and specificity of this uterine scoring system is quite good. It could be a good tool in assessing endometrium before embryo transfer.

Trial registration number
Not applicable
P-585 Iron intake in relation to ovarian reserve among women seeking infertility treatment
Authors: Chavarro J , Jimenez-Cardozo N , Mitsunami M , Minguez-Alarcon L , Ortiz-Panozo E , Wang S , Hauser R , Souter I 
Published: June 2023

Abstract

Study question
Is there an association between iron intake and ovarian reserve among women seeking fertility care?

Summary answer
Supplemental iron intake above 45mg/d is associated with lower ovarian reserve among women seeking fertility care.

What is known already
Although the literature regarding iron intake in relation to ovarian reserve is scant and inconsistent, some evidence suggest that iron may have gonadotoxic effects.

Study design, size, duration
Observational study including 582 female participants attending the Massachusetts General Hospital Fertility Center (2007–2019) enrolled in the Environment and Reproductive Health (EARTH) Study

Participants/materials, setting, methods
Iron intake was estimated using a validated food frequency questionnaire (FFQ). Markers of ovarian reserve included antral follicle count (AFC) (assessed via transvaginal ultrasound) and day 3 FSH, both obtained during the course of infertility evaluation. The association between iron intake and ovarian reserve was evaluated using Poisson regression models for AFC and quantile regression models for day 3 FSH adjusted for age, menstrual cycle characteristics, physical activity, carbohydrate intake and total energy intake.

Main results and the role of chance
Participants had a median age of 35 years and median total iron intake of 29 mg/d. Total iron intake was inversely related to AFC and this association was driven by intake of supplemental iron. Compared to women with a supplemental iron intake ≤20mg/day, women consuming 45-64mg/d of supplemental iron had a 17% (-35%, 0.3%) lower AFC and women consuming ≥65mg/d of supplemental iron had a 32% (-54%, -11%) lower AFC after adjusting for potential confounders (p, linear trend=0.003). Similarly, in multivariable-adjusted analysis, day 3 FSH levels were 0.9 (0.5, 1.3) IU/mL higher among women with a supplemental iron intake ≥65mg/d when compared to women with a supplemental iron intake ≤20mg/d (p, linear trend=0.02).

Limitations, reasons for caution
Iron intake was estimated using a method that relies on self-report and we had no biomarkers of iron status in our participants. Also, only 36 women consumed ≥45mg/d of supplemental iron.

Wider implications of the findings
Since all study participants were seeking fertility treatment, our findings may not apply to women in the general population. Although our findings are consistent with studies of women with iron overload, given the paucity of literature on this topic it is essential that this question is revisited in future studies.

Trial registration number
Not applicable, not a randomized trial
P-591 Follicular fluid-derived exosomes rejuvenate ovarian aging through miR-320a-3p-mediated FOXQ1 inhibition
Authors: Liu Y , Yan L , Xixiang M , Wenpei X 
Published: June 2023

Abstract

Study question
Dose the follicular fluid-derived exosomes rejuvenate ovarian aging?

Summary answer
Follicular fluid-derived exosomes efficiently rescued ovarian function in aged mice, which was achieved via transfer of miR-320-3p to granulosa cells and subsequent Foxq1 inhibition.

What is known already
The ovary enters the malfunctional aging phase earlier and faster than other organs and systems. Ovarian aging is mainly characterized by a progressive decline in oocyte quantity and quality, which ultimately leads to female infertility. Various therapies have been established to cope with age-related ovarian dysfunction, among which exosome-based therapy has been considered a promising strategy that can benefit ovarian functions via multiple pathways.

Study design, size, duration
Ovarian granulosa cells of young C57BL/6J mice (6-8 w) and old C57BL/6J mice (40 w) were cultured in vitro. The follicular fluid exosomes of young mice and the same amount of PBS were respectively injected into the ovarian sac of old mice.

Participants/materials, setting, methods
Cell proliferation and apoptosis were detected by CCK8, flow cytometry and Western Blot. Mitochondrial function was assessed by measuring mitochondrial membrane potential, ATP levels, mtDNA copy numbers. The number of ovarian follicles were observed by HE staining. Senescence and telomere related gene expression levels were detected by RT-qPCR. 2-cell rates and blastocyst formation rates were observed after in vitro fertilization. The model mice were directly cooped up to observe the pregnat and litter sizes.

Main results and the role of chance
The follicular fluid exosomes from young mice can promote the proliferation of ovarian granulosa cells from old mice and improve mitochondrial function. When the follicular fluid exosomes from young mice were injected into the ovarian sac of old mice, the number of primordial and growing follicles in the ovaries of old mice were increased and the developmental ability of oocytes was improved.

Limitations, reasons for caution
Findings in mouse.

Wider implications of the findings
This study suggests that young follicular fluid exosomes may be involved in ovarian senescence by transferring miR-320a-3p targeting FOXQ1 into ovarian granulosa cells of aged mice.

Trial registration number
no
P-613 Deletion of the CLPP gene in oocytes exacerbates chemotherapy-induced ovarian damage
Authors: Gu J , Guo C , Xiao Y , Hua R , Hai Z , Zhao P , Su J , Wang T 
Published: June 2023

Abstract

Study question
We attempted to investigate the role of the Clpp in chemotherapy-induced ovarian damage and the decline of oocyte function

Summary answer
We clarified that the deletion of Clpp gene in oocytes will aggravate the ovarian damage and the decline of oocyte function caused by chemotherapeutic drugs.

What is known already
Chemotherapy is extensively used to treat cancers and is often associated with ovarian damage which leads to premature ovarian insufficiency and infertility. Mitochondria have the critical role of producing energy and maintaining normal cellular activities for oocyte maturation and folliculogenesis.

Study design, size, duration
This study used a mouse model with oocyte-specific deletion of mitochondrial stress response gene Caseinolytic peptidase P (Clpp) to investigate mitochondrial homeostasis in oocytes from mice receiving a chemotherapeutic drug cyclophosphamide (CTX).

Participants/materials, setting, methods
First, analyze the reproductive phenotype and fertility test of mice, observe the ovarian development, the proportion of follicles at all levels, the spindle of oocytes and the aneuploidy rate of chromosomes at the MII stage, and using multiple mitochondrial function indicator kit to detect mitochondrial function. Then, we modeled 8-week-old mice with a single injection of 100ul 75uM cyclophosphamide. After 14 days, check the above indicators again. the impact of cyclophosphamide modeling will be analyzed.

Main results and the role of chance
We found the specific deletion of Clpp in oocytes reduced fecundity of the mice at advanced age. The Clpp deletion led to meiotic defects and impaired mitochondrial distribution and function in the MII oocytes. CTX induction further affected oocyte competence in Clpp cKO mice and lead to an exhaustive disruption of blastocytes formation due to the severe effects on mitochondrial distributions and functions.

Limitations, reasons for caution
We only clarified that the deletion of Clpp gene in oocytes will through affecting the mitochondrial function aggravate the ovarian damage and the decline of oocyte function caused by chemotherapeutic drugs.But the underlying mechanism has not been able to explore further.

Wider implications of the findings
This is first study specifically on the impact of oocyte specific deletion of Clpp on oocyte competence and early embryo development. At the same time, the role of Clpp gene in the ovary damaged by chemotherapy drugs was further clarified.

Trial registration number
not applicable
P-614 Endometrial preparation: effect of estrogen levels before the embryo transfer on the live birth rate from 14825 freezing-all cycles
Authors: Li Y , Xiaojuan W , Ge L , Fei G 
Published: June 2023

Abstract

Study question
Is there a difference in live birth rate during discrepant estrogen levels before the embryo transfer following either hormone replacement therapy (HRT) or natural cycle(NC)?

Summary answer
The higher estrogen level on the day before embryo transfer may reduce the live birth rate in either HRT or NC.

What is known already
The endometrial preparation of frozen embryo transfer cycle is to synchronize of endometrium and embryo under the condition of exogenous estrogen and progesterone drugs in HRT or follicle monitoring in NC. Estrogen is crucial and different estrogen levels could imply variations on endometrial preparation. Information on the effect of discrepant estrogen levels before the embryo transfer on reproductive outcomes of women undergoing freezing-all cycles is scarce.

Study design, size, duration
Retrospective cohort study including 14825 first frozen embryo transfers following IVF/ICSI undergoing freezing-all cycles from January 2016 to December 2020. A total of 12305 (83.0%) patients received NC while 2520 (17.0%) received HRT.

Participants/materials, setting, methods
Effect of estrogen levels on live birth rates, stratifified by deciles and ROC to determine the cutoff values for estrogen level on the day before embryo transfer.Multivariable logistic regression analysis was performed to adjust for confounders.

Main results and the role of chance
The live birth rate was 44.7% and 52.4% in HRT and NC respectively. The live birth rate of HRT began to decline when the estrogen level was P60 in the deciles which the cut-off values of ROC was closed with the estrogen level was 891.6 pg/ml. The lower level of estrogen on the day before embryo transfer in HRT corresponds to a higher live birth rate（51.4% vs 32.9%）.The cut-off values of ROC in NC was statistically different with the estrogen level was 194.4 pg/ml. The lower level of estrogen on the day before embryo transfer in NC corresponds to a higher live rate（53.0% vs 48.6%）.After adjusting the possible confounding factors by multivariate logistic regression, it was found that the estrogen level on the day before embryo transfer in HRT and NC was the independent influencing factor of live birth rate with the OR of 0.57 (0.47,0.68) and 0.82 (0.74,0.90), respectively.

Limitations, reasons for caution
The greatest limitation of this study is its retrospective study. On the other hand, this study was performed using non-PGT cycle, although this is unlikely to affect the results,we cannot exclude the possibility that euploid embryo responds differently to endometrial state in comparison to aneuploid embryo.

Wider implications of the findings
In freezing-all cycles, the higher estrogen level on the day before embryo transfer may reduce the live birth rate in either HRT or NC. Clinicians should pay attention to the control of estrogen level in luteal phase.

Trial registration number
Nona
P-615 Ovarian rejuvenation with human placenta hydrolyzate
Authors: Rybina A , Askar E , Abshekenova A , Karibayeva S , Valiev R , Lokshin V 
Published: June 2023

Abstract

Study question
Is it possible to rejuvenate the ovaries with human placenta hydrolyzate?

Summary answer
Injecting human placenta hydrolyzate into the ovaries can activate dormant follicles and produce mature oocytes in patients with ovarian insufficiency.

What is known already
Patients with low ovarian reserve and/or ovarian failure are a difficult category for reproductive specialists. Attempts to rejuvenate the ovaries have been made for several years. The use of adjuvant therapy such as PRP can activate dormant follicles. Human placenta hydrolyzate contains growth factors, hormones, trace elements, vitamins. Administration of human placenta hydrolyzate directly to the ovaries can also activate folliculogenesis and oogenesis.

Study design, size, duration
A cohort study was conducted, which involved 20 women with low ovarian reserve who underwent IVF / ICSI at the Person ICCR in 2022. AMH, FSH, the number of antral follicles, the number of received oocytes were determined before the injection of the human placenta hydrolyzate (JAPAN BIO PRODUCTS Co, LTD, Japan) into the ovaries and 1-3 months after procedures.

Participants/materials, setting, methods
All patients had previously undergone IVF/ICSI. 20 women with infertility, mean age was 39±6 years. In September-October 2022, we received a human placenta hydrolyzate intraovarially. The human placenta hydrolyzate was injected into the ovaries as in a transvaginal puncture with a 20 G needle, 1 ml into each ovary. All participants gave their voluntary informed consent to the procedure.

Main results and the role of chance
On the 2-3rd day of the cycle before the procedure, the average level of AMH was 0.11±0.09 ng/ml, FSH 38.11±10.86 mIU/ml, the number of antral follicles 1.18±0, 38, the number of mature oocytes obtained on TVP, 0.68±0.19. After the introduction of the human placenta hydrolyzate into the ovaries, measurements were repeated 1-3 months later: AMH was 0.46±0.18 ng/ml, p = 0.09; FSH was 13.54±7.86 mIU/ml, p = 0.074; CAF 1.96±0.17, p = 0.068; oocytes M2 1.51±0.38, p = 0.058. Due to small cohort size and short follow-up time, we believe that ovarian activation by placental hydrolyzate takes place, this is especially noticeable in the number of obtained mature oocytes that went for freezing or fertilization: 4 patients received embryos suitable for PGT-A, one the embryo (mosaic) was transferred into the uterine cavity and pregnancy occurred. There was no any side effect after the injection.

Limitations, reasons for caution
Limitations relate to study design. More patients and longer follow-up are also needed.

Wider implications of the findings
Ovarian rejuvenation is currently an adjuvant therapy, high-level evidence is not yet available. However, for women with POI and POR, any opportunity to improve the chances of obtaining their own oocytes is valuable.

Trial registration number
NO APPLICABLE
P-627 Effects of ntraovarian Injection of Autologous Platelet-Rich Plasma on Ovarian Reserve in Poor Responders undergoing IVF Treatment
Authors: Gao F , Min F , Yanbin W 
Published: June 2023

Abstract

Study question
Does intracortical injections of autologous platelet-rich plasma (PRP) improve the outcomes in patients with poor ovarian response (POR) undergoing the IVF treatment?

Summary answer
PRP injections could improve the markers of low ovarian reserve, especially the first month after the injection.

What is known already
Poor ovarian responders (PORs) with poor reproductive outcomes during the IVF treatment is considered the main challenges of reproductive science. PORs are presented by low ovarian reserve, poor oocyte quality and low response to ovarian stimulation protocols. Despite several approaches that have been investigated to improve the ovulation outcome in PORs, the pregnancy rate remains low.

Study design, size, duration
The pilot study was designed as prospective observational studies. 39 PORs based on Bologna criteria were included and followed by 3 months. Couples presenting with any other infertility etiology other than POR were excluded. All participants provided written informed consent prior to treatment.

Participants/materials, setting, methods
The patients in the study were received at least two previous failed Intracytoplasmic Sperm Injection (ICSI)-Embryo Transfer (ET) fresh cycle. PRP intraovarian infusion treatment was performed following the last failed ICSI-ET cycle. The patients were followed 3 months after the injection. Primary outcome measures were levels of anti-müllerian hormone (AMH), antral follicle count (AFC), follicle stimulating hormone (FSH), and oocyte yield after the PRP treatment.

Main results and the role of chance
The level of AMH was significantly elevated from 0.52±0.43ng/ml before the PRP treatment to 0.82±0.61ng/ml after the treatment. The AFC showed the same trend from 2.52±1.50 to 5.83±4.25 and the FSH level was decreased by 31.98%. But the number of retrieved oocytes and embryo available were not significantly increased.

Limitations, reasons for caution
The design of the observational studies and the small sample size.

Wider implications of the findings
PRP injection may be a potiental treatment for PORs in IVF.

Trial registration number
not applicable
Generalized Chromatic Functions
Authors: Aliniaeifard Farid , Li Shu Xiao , van Willigenburg Stephanie 
Published: June 2023

Abstract
We define vertex-colourings for edge-partitioned digraphs, which unify the theory of $P$-partitions and proper vertex-colourings of graphs. We use our vertex-colourings to define generalized chromatic functions, which merge the chromatic symmetric and quasisymmetric functions of graphs and generating functions of $P$-partitions. Moreover, numerous classical bases of symmetric and quasisymmetric functions, both in commuting and noncommuting variables, can be realized as special cases of our generalized chromatic functions. We also establish product and coproduct formulas for our functions. Additionally, we construct the new Hopf algebra of $r$-quasisymmetric functions in noncommuting variables, and apply our functions to confirm its Hopf structure, and establish natural bases for it.
Lessons (Machine) Learned From COVID-19
Authors: Sullivan Timothy 
Published: June 2023

Abstract
Since coronavirus disease 2019 (COVID-19) first emerged more than 3 years ago, more than 1200 articles have been written describing “lessons learned” from the pandemic. While these articles may contain valuable insights, reading them all would be impossible. A machine learning clustering analysis was therefore performed to obtain an overview of these publications and to highlight the benefits of using machine learning to analyze the vast and ever-growing COVID-19 literature.
RNA Editing as a General Trait of Ebolaviruses
Authors: Mehedi Masfique , Ricklefs Stacy , Takada Ayato , Sturdevant Dan , Porcella Stephen F , Marzi Andrea , Feldmann Heinz 
Published: June 2023

Abstract
RNA editing has been discovered as an essential mechanism for the transcription of the glycoprotein (GP) gene of Ebola virus but not Marburg virus. We developed a rapid transcript quantification assay (RTQA) to analyze RNA transcripts generated through RNA editing and used immunoblotting with a pan-ebolavirus monoclonal antibody to confirm different GP gene–derived products. RTQA successfully quantified GP gene transcripts during infection with representative members of 5 ebolavirus species. Immunoblotting verified expression of the soluble GP and the transmembrane GP. Our results defined RNA editing as a general trait of ebolaviruses. The degree of editing, however, varies among ebolaviruses with Reston virus showing the lowest and Bundibugyo virus the highest degree of editing.
Limited evidence for extensive genetic differentiation between X and Y chromosomes in Hybognathus amarus (Cypriniformes: Leuciscidae)
Authors: Caeiro-Dias Guilherme , Osborne Megan J , Waterman Hannah M , Krabbenhoft Trevor J , Turner Thomas F 
Published: June 2023

Abstract
Sex determination systems and genetic sex differentiation across fishes are highly diverse but are unknown for most Cypriniformes, including Rio Grande silvery minnow (Hybognathus amarus). In this study, we aimed to detect and validate sex-linked markers to infer sex determination system and to demonstrate the utility of combining several methods for sex-linked marker detection in nonmodel organisms. To identify potential sex-linked markers, Nextera-tagmented reductively amplified DNA (nextRAD) libraries were generated from 66 females, 64 males, and 60 larvae of unknown sex. These data were combined with female and male de novo genomes from Nanopore long-read sequences. We identified five potential unique male nextRAD-tags and one potential unique male contig, suggesting an XY sex determination system. We also identified two single-nucleotide polymorphisms (SNPs) in the same contig with values of FST, allele frequencies, and heterozygosity conforming with expectations of an XY system. Through PCR we validated the marker containing the sex-linked SNPs and a single nextRAD-tag sex-associated marker but it was not male specific. Instead, more copies of this locus in the male genome were suggested by enhanced amplification in males. Results are consistent with an XY system with low differentiation between sex-determining regions. Further research is needed to confirm the level of differentiation between the sex chromosomes. Nonetheless, this study highlighted the power of combining reduced representation and whole-genome sequencing for identifying sex-linked markers, especially when reduced representation sequencing does not include extensive variation between sexes, either because such variation is not present or not captured.