bluuebunny/crossref_metadata_2025_split huggingface dataset:
Giant multiphononic effects in a perovskite oxide
Authors: Cazorla Claudio , Stengel Massimiliano , Íñiguez Jorge , Rurali Riccardo 
Published: June 2023

AbstractPerovskite oxides offer tremendous potential for applications in information storage and energy conversion, owing to a subtle interplay between their spin, charge, orbital and lattice degrees of freedom. Here, we further expand the possible range of perovskite oxides operation towards the fields of thermal management and thermal computing by exploiting an exceptional synergy between different ferroic orders. We propose dynamical control of the heat flow in a distinctive family of perovskite oxides obtained via the application of small electric (~10 kV/cm) and/or magnetic (~1 T) fields. Based on first-principles simulations, we predict a relative heat conductivity variation of ~100% in SrMnO3 thin films near room temperature resulting from a phase transition that involves huge changes in both the magnetization and electric polarization. The disclosed giant multiphononic effects are fundamentally caused by anharmonic spin-phonon couplings that strongly influence the mean lifetime of phonons.
Perfect Crystals: microgravity capillary counterdiffusion crystallization of human manganese superoxide dismutase for neutron crystallography
Authors: Lutz William E. , Azadmanesh Jahaun , Lovelace Jeffrey J. , Kolar Carol , Coates Leighton , Weiss Kevin L. , Borgstahl Gloria E. O. 
Published: June 2023

AbstractThe NASA mission Perfect Crystals used the microgravity environment on the International Space Station (ISS) to grow crystals of human manganese superoxide dismutase (MnSOD)—an oxidoreductase critical for mitochondrial vitality and human health. The mission’s overarching aim is to perform neutron protein crystallography (NPC) on MnSOD to directly visualize proton positions and derive a chemical understanding of the concerted proton electron transfers performed by the enzyme. Large crystals that are perfect enough to diffract neutrons to sufficient resolution are essential for NPC. This combination, large and perfect, is hard to achieve on Earth due to gravity-induced convective mixing. Capillary counterdiffusion methods were developed that provided a gradient of conditions for crystal growth along with a built-in time delay that prevented premature crystallization before stowage on the ISS. Here, we report a highly successful and versatile crystallization system to grow a plethora of crystals for high-resolution NPC.
Impact of timing and combination of different BNT162b2 and ChAdOx1-S COVID-19 basic and booster vaccinations on humoral immunogenicity and reactogenicity in adults
Authors: Dedroogh Simon , Schmiedl Sven , Thürmann Petra A. , Graf Katharina , Appelbaum Sebastian , Koß Reinhard , Theis Christian , Zia Zewarudin , Tebbenjohanns Jürgen , Thal Serge C. , Dedroogh Michael 
Published: June 2023

AbstractIn this single-center observational study with 1,206 participants, we prospectively evaluated SARS-CoV-2-antibodies (anti-S RBD) and vaccine-related adverse drug reactions (ADR) after basic and booster immunization with BNT162b2- and ChAdOx1-S-vaccines in four vaccination protocols: Homologous BNT162b2-schedule with second vaccination at either three or six weeks, homologous ChAdOx1-S-vaccination or heterologous ChAdOx1-S/BNT162b2-schedule, each at 12 weeks. All participants received a BNT162b2 booster. Blood samples for anti-S RBD analysis were obtained multiple times over a period of four weeks to six months after basic vaccination, immediately before, and up to three months after booster vaccination. After basic vaccination, the homologous ChAdOx1-S-group showed the lowest anti-S RBD levels over six months, while the heterologous BNT162b2-ChAdOx1-S-group demonstrated the highest anti-S levels, but failed to reach level of significance compared with the homologous BNT162b2-groups. Antibody levels were higher after an extended vaccination interval with BNT162b2. A BNT162b2 booster increased anti-S-levels 11- to 91-fold in all groups, with the homologous ChAdOx1-S-cohort demonstrated the highest increase in antibody levels. No severe or serious ADR were observed. The findings suggest that a heterologous vaccination schedule or prolonged vaccination interval induces robust humoral immunogenicity with good tolerability. Extending the time to boost-immunization is key to both improving antibody induction and reducing ADR rate.
Exploring the cytotoxicity on human lung cancer cells and DNA binding stratagem of camptothecin functionalised silver nanoparticles through multi-spectroscopic, and calorimetric approach
Authors: Raj Aparna , Thomas Riju K. , Vidya L. , Aparna V. M. , Neelima S. , Sudarsanakumar C. 
Published: June 2023

AbstractThe influence of nanoparticles inside the human body and their interactions with biological macromolecules need to be explored/studied prior to specific applications. The objective of this study is to find the potential of camptothecin functionalised silver nanoparticles (CMT-AgNPs) in biomedical applications. This article primarily investigates the binding stratagem of CMT-AgNPs with calf thymus DNA (ctDNA) through a series of spectroscopic and calorimetric methods and then analyses the anticancer activity and cytotoxicity of CMT-AgNPs. The nanoparticles were synthesized using a simple one pot method and characterized using UV–Visible, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction and high-resolution transmission electron microscopy (HRTEM). The average size of CMT-AgNPs is 10 ± 2 nm. A group of experimental techniques such as UV–Visible spectrophotometry, fluorescence dye displacement assay, circular dichroism (CD) and viscosity analysis unravelled the typical groove binding mode of CMT-AgNPs with ctDNA. The CD measurement evidenced the minor conformational alterations of double helical structure of ctDNA in the presence of CMT-AgNPs. The information deduced from the isothermal titration calorimetry (ITC) experiment is that the binding was exothermic and spontaneous in nature. Moreover, all the thermodynamic binding parameters were extracted from the ITC data. The binding constants obtained from UV absorption experiments, fluorescence dye displacement studies and ITC were consistently in the order of 104 Mol−1. All these results validated the formation of CMT-AgNPs–ctDNA complex and the results unambiguously confirm the typical groove binding mode of CMT-AgNPs. An exhaustive in vitro MTT assay by CMT-AgNPs and CMT against A549, HT29, HeLa and L929 cell lines revealed the capability of CMT-AgNPs as a potential anticancer agent.
Reverse transcriptase recombinase polymerase amplification for detection of tomato spotted wilt orthotospovirus from crude plant extracts
Authors: Iturralde Martinez Juan Francisco , Rosa Cristina 
Published: June 2023

AbstractVirus detection in early stages of infection could prove useful for identification and isolation of foci of inoculum before its spread to the rest of susceptible individuals via vectoring insects. However, the low number of viruses present at the beginning of infection renders their detection and identification difficult and requires the use of highly sensitive laboratory techniques that are often incompatible with a field application. To obviate this challenge, utilized Recombinase Polymerase Amplification, an isothermal amplification technique that makes millions of copies of a predefined region in the genome, to detect tomato spotted wilt orthotospovirus in real time and at the end point. The reaction occurs isothermically and can be used directly from crude plant extracts without nucleic acid extraction. Notably, a positive result can be seen with the naked eye as a flocculus made of newly synthesized DNA and metallic beads. The objective of the procedure is to create a portable and affordable system that can isolate and identify viruses in the field, from infected plants and suspected insect vectors, and can be used by scientists and extension managers for making informed decisions for viral management. Results can be obtained in situ without the need of sending the samples to a specialized lab.
Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6
Authors: Purwaningrum Medania , Giachelli Cecilia M. , Osathanon Thanaphum , Rattanapuchpong Sirirat , Sawangmake Chenphop 
Published: June 2023

AbstractPeriodontal ligament stem cells (PDLSCs) play a significant role on periodontal tissue and alveolar bone homeostasis. During inflammation, interleukin (IL)-6 serves as one of key cytokine players controlling tissue reaction as well as alveolar bone tissue remodeling. It is believed that periodontal tissue inflammation causes periodontium degradation, especially alveolar bone. However, in this study, we show that an inflammatory mediator, IL-6, may serve another direction on alveolar bone homeostasis during inflammatory condition. We found that, IL-6 at 10 and 20 ng/mL was not cytotoxic and dose-dependently exerted beneficial effects on osteogenic differentiation of human PDLSCs (hPDLSCs), as demonstrated by increased alkaline phosphatase activity, mRNA expression of osteogenic markers, and matrix mineralization. The presence of physiological and inflammatory level of IL-6, the osteogenic differentiation potential by hPDLSCs was enhanced by several possible mechanisms including transforming growth factor (TGF), Wnt, and Notch pathways. After in-depth and thorough exploration, we found that Wnt pathway serves as key regulator controlling osteogenic differentiation by hPDLSCs amid the IL-6 presentation. Surprisingly, apart from other mesenchymal stem cells, distinct Wnt components are employed by hPDLSCs, and both canonical and non-canonical Wnt pathways are triggered by different mechanisms. Further validation by gene silencing, treatment with recombinant Wnt ligands, and β-catenin stabilization/translocation confirmed that IL-6 governed the canonical Wnt/β-catenin pathway via either WNT2B or WNT10B and employed WNT5A to activate the non-canonical Wnt pathway. These findings fulfill the homeostasis pathway governing periodontal tissue and alveolar bone regeneration and may serve for further therapeutic regimen design for restoring the tissues.
Effect of breed and diet on the M. longissimus thoracis et lumborum transcriptome of steers divergent for residual feed intake
Authors: Keogh Kate , McKenna Clare , Waters Sinead M. , Porter Richard K. , Fitzsimons Claire , McGee Mark , Kenny David A. 
Published: June 2023

AbstractImproving cattle feed efficiency through selection of residual feed intake (RFI) is a widely accepted approach to sustainable beef production. A greater understanding of the molecular control of RFI in various breeds offered contrasting diets is necessary for the accurate identification of feed efficient animals and will underpin accelerated genetic improvement of the trait. The aim of this study was to determine genes and biological processes contributing to RFI across varying breed type and dietary sources in skeletal muscle tissue. Residual feed intake was calculated in Charolais and Holstein–Friesian steers across multiple dietary phases (phase-1: high concentrate (growing-phase); phase-2: zero-grazed grass (growing-phase); phase-3: high concentrate (finishing-phase). Steers divergent for RFI within each breed and dietary phase were selected for muscle biopsy collection, and muscle samples subsequently subjected to RNAseq analysis. No gene was consistently differentially expressed across the breed and diet types examined. However, pathway analysis revealed commonality across breeds and diets for biological processes including fatty acid metabolism, immune function, energy production and muscle growth. Overall, the lack of commonality of individual genes towards variation in RFI both within the current study and compared to the published literature, suggests other genomic features warrant further evaluation in relation to RFI.
Model-based estimation of muscle and ACL forces during turning maneuvers in alpine skiing
Authors: Heinrich Dieter , van den Bogert Antonie J. , Mössner Martin , Nachbauer Werner 
Published: June 2023

AbstractIn alpine skiing, estimation of the muscle forces and joint loads such as the forces in the ACL of the knee are essential to quantify the loading pattern of the skier during turning maneuvers. Since direct measurement of these forces is generally not feasible, non-invasive methods based on musculoskeletal modeling should be considered. In alpine skiing, however, muscle forces and ACL forces have not been analyzed during turning maneuvers due to the lack of three dimensional musculoskeletal models. In the present study, a three dimensional musculoskeletal skier model was successfully applied to track experimental data of a professional skier. During the turning maneuver, the primary activated muscles groups of the outside leg, bearing the highest loads, were the gluteus maximus, vastus lateralis as well as the medial and lateral hamstrings. The main function of these muscles was to generate the required hip extension and knee extension moments. The gluteus maximus was also the main contributor to the hip abduction moment when the hip was highly flexed. Furthermore, the lateral hamstrings and gluteus maximus contributed to the hip external rotation moment in addition to the quadratus femoris. Peak ACL forces reached 211 N on the outside leg with the main contribution in the frontal plane due to an external knee abduction moment. Sagittal plane contributions were low due to consistently high knee flexion (> 60$$^{\circ }$$

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), substantial co-activation of the hamstrings and the ground reaction force pushing the anteriorly inclined tibia backwards with respect to the femur. In conclusion, the present musculoskeletal simulation model provides a detailed insight into the loading of a skier during turning maneuvers that might be used to analyze appropriate training loads or injury risk factors such as the speed or turn radius of the skier, changes of the equipment or neuromuscular control parameters.
Pneumonia detection with QCSA network on chest X-ray
Authors: Singh Sukhendra , Kumar Manoj , Kumar Abhay , Verma Birendra Kumar , Shitharth S. 
Published: June 2023

AbstractWorldwide, pneumonia is the leading cause of infant mortality. Experienced radiologists use chest X-rays to diagnose pneumonia and other respiratory diseases. The diagnostic procedure's complexity causes radiologists to disagree with the decision. Early diagnosis is the only feasible strategy for mitigating the disease's impact on the patent. Computer-aided diagnostics improve the accuracy of diagnosis. Recent studies established that Quaternion neural networks classify and predict better than real-valued neural networks, especially when dealing with multi-dimensional or multi-channel input. The attention mechanism has been derived from the human brain's visual and cognitive ability in which it focuses on some portion of the image and ignores the rest portion of the image. The attention mechanism maximizes the usage of the image's relevant aspects, hence boosting classification accuracy. In the current work, we propose a QCSA network (Quaternion Channel-Spatial Attention Network) by combining the spatial and channel attention mechanism with Quaternion residual network to classify chest X-Ray images for Pneumonia detection. We used a Kaggle X-ray dataset. The suggested architecture achieved 94.53% accuracy and 0.89 AUC. We have also shown that performance improves by integrating the attention mechanism in QCNN. Our results indicate that our approach to detecting pneumonia is promising.
Comparable responses to a wide range of olfactory stimulation in women and men
Authors: Lillqvist Moa , Claeson Anna-Sara , Zakrzewska Marta , Andersson Linus 
Published: June 2023

AbstractThe evidence for differences between women and men in terms of olfactory abilities is contradictory. We analyzed women and men’s performance and reactions to a wider range of odour exposure outcomes than usually studied, to assess possible differences and similarities between sexes. Measures of sensitivity and sensory decision rule were established in 37 women and 39 men. Perceptual, cognitive, symptom-related and autonomic nervous system (skin conductance level and heart-rate variability) reactions were also assessed during extended ambient odour exposure, as well as participants’ self-rated chemical intolerance. Bayesian analyses consistently revealed greater support for sex-related similarities than differences, suggesting that women and men perform and react comparably not only in terms of basic olfactory measures, but also to environmental odour exposure mimicking everyday situations.
Delta weight loss unlike genetic variation associates with hyperoxaluria after malabsorptive bariatric surgery
Authors: Scherer Lotte , Schönauer Ria , Nemitz-Kliemchen Melanie , Hagemann Tobias , Hantmann Elena , de Fallois Jonathan , Petzold Friederike , Blüher Matthias , Halbritter Jan 
Published: June 2023

AbstractThe risk of enteric hyperoxaluria is significantly increased after malabsorptive bariatric surgery (MBS). However, its underlying determinants are only poorly characterized. In this case–control study, we aimed at identifying clinical and genetic factors to dissect their individual contributions to the development of post-surgical hyperoxaluria. We determined the prevalence of hyperoxaluria and nephrolithiasis after MBS by 24-h urine samples and clinical questionnaires at our obesity center. Both hyperoxaluric and non-hyperoxaluric patients were screened for sequence variations in known and candidate genes implicated in hyperoxaluria (AGXT, GRHPR, HOGA1, SLC26A1, SLC26A6, SLC26A7) by targeted next generation sequencing (tNGS). The cohort comprised 67 patients, 49 females (73%) and 18 males (27%). While hyperoxaluria was found in 29 patients (43%), only one patient reported postprocedural nephrolithiasis within 41 months of follow-up. Upon tNGS, we did not find a difference regarding the burden of (rare) variants between hyperoxaluric and non-hyperoxaluric patients. However, patients with hyperoxaluria showed significantly greater weight loss accompanied by markers of intestinal malabsorption compared to non-hyperoxaluric controls. While enteric hyperoxaluria is very common after MBS, genetic variation of known hyperoxaluria genes contributes little to its pathogenesis. In contrast, the degree of postsurgical weight loss and levels of malabsorption parameters may allow for predicting the risk of enteric hyperoxaluria and consecutive kidney stone formation.
Comparative analysis of bats and rodents’ genomes suggests a relation between non-LTR retrotransposons, cancer incidence, and ageing
Authors: Ricci Marco , Peona Valentina , Boattini Alessio , Taccioli Cristian 
Published: June 2023

AbstractThe presence in nature of species showing drastic differences in lifespan and cancer incidence has recently increased the interest of the scientific community. In particular, the adaptations and the genomic features underlying the evolution of cancer-resistant and long-lived organisms have recently focused on transposable elements (TEs). In this study, we compared the content and dynamics of TE activity in the genomes of four rodent and six bat species exhibiting different lifespans and cancer susceptibility. Mouse, rat, and guinea pig genomes (short-lived and cancer-prone organisms) were compared with that of naked mole rat (Heterocephalus glaber) which is a cancer-resistant organism and the rodent with the longest lifespan. The long-lived bats of the genera Myotis, Rhinolophus, Pteropus and Rousettus were instead compared with Molossus molossus, which is one of the organisms with the shortest lifespan among the order Chiroptera. Despite previous hypotheses stating a substantial tolerance of TEs in bats, we found that long-lived bats and the naked mole rat share a marked decrease of non-LTR retrotransposons (LINEs and SINEs) accumulation in recent evolutionary times.
Construction and characterization of nano-oval BaTi0.7Fe0.3O3@NiFe2O4 nanocomposites as an effective platform for the determination of H2O2
Authors: Hammad Ali B. Abou , Magar Hend S. , Mansour A. M. , Hassan Rabeay Y. A. , Nahrawy Amany M. El 
Published: June 2023

AbstractTalented di-phase ferrite/ferroelectric BaTi0.7Fe0.3O3@NiFe2O4 (BFT@NFO) in oval nano-morphology was chemically synthesized using controlled sol–gel processes and calcined at 600 °C. The effects of shielding using NiFe2O4 (NFO) nanoparticles on the microstructure, phase transition, thermal, and relative permittivity of BaTi0.7Fe0.3O3 (BTF) nano-perovskite were systematically explored. X-ray diffraction patterns and Full-Prof software exhibited the forming of the BaTi2Fe4O11 hexagonal phase. TEM and SEM images demonstrated that the coating of BaTi0.7Fe0.3O3 has been successfully controlled with exquisite nano-oval NiFe2O4 shapes. The NFO shielding can significantly promote the thermal stability and the relative permittivity of BFT@NFO pero-magnetic nanocomposites and lowers the Curie temperature. Thermogravimetric and optical analysis were used to test the thermal stability and estimate the effective optical parameters. Magnetic studies showed a decrease in saturation magnetization of NiFe2O4 NPs compared to their bulk system, which is attributed to surface spin disorder. Herein, characterization and the sensitive electrochemical sensor were constructed for the evaluation of peroxide oxidation detection using the chemically adjusted nano-ovals barium titanate-iron@nickel ferrite nanocomposites. Finally, The BFT@NFO exhibited excellent electrochemical properties which can be ascribed to this compound possessing two electrochemical active components and/or the nano-ovals structure of the particles which can further improve the electrochemistry through the possible oxidation states and the synergistic effect. The result advocates that when the BTF is shielded with NFO nanoparticles the thermal, dielectric, and electrochemical properties of nano-oval BaTi0.7Fe0.3O3@NiFe2O4 nanocomposites can be synchronously developed. Thus, the production of ultrasensitive electrochemical nano-systems for the determination of hydrogen peroxide is of extensive significance.