bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelial sensing of AHR ligands regulates intestinal homeostasis
Authors: Wiggins Benjamin G. , Wang Yi-Fang , Burke Alice , Grunberg Nil , Walker Julia M. Vlachaki , Dore Marian , Chahrour Catherine , Pennycook Betheney , Sanchez-Garrido Julia , Vernia Santiago , Barr Alexis R. , Frankel Gad , Birdsey Graeme M. , Randi Anna M. , Schiering Chris 
Published: April 2023

Abstract
The blood and lymphatic vasculature is lined by functionally specialised endothelial cells (ECs). Vascular beds act as an essential physical barrier, control nutrient transport, facilitate tissue immunosurveillance, and coordinate angiogenesis and lymphangiogenesis to ensure appropriate tissue perfusion and drainage1,2. Conversely, vascular maladaptation can lead to pathological angiogenesis and the perpetuation of inflammation in chronic inflammatory diseases3,4. In the intestine, dietary and microbial cues are particularly important in regulation of organ homeostasis. However, whether enteric ECs actively sense and integrate such signals is currently unknown. Here, we show that the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, acts as critical node for EC-sensing of dietary metabolites in adult mice and human primary ECs. We first established a comprehensive single-cell endothelial atlas of the mouse small intestine, uncovering the cellular complexity and functional heterogeneity of blood and lymphatic ECs. Analyses of AHR mediated responses at single-cell resolution identified tissue-protective transcriptional signatures and regulatory networks promoting cellular quiescence and vascular normalcy at steady state. Endothelial AHR-deficiency in adult mice resulted in dysregulated inflammatory responses, and initiation of proliferative and angiogenic pathways. Furthermore, endothelial sensing of dietary AHR ligands was required for optimal protection against enteric infection. In human ECs, AHR signalling promoted quiescence and restrained activation by inflammatory mediators. Together, our data provide a comprehensive dissection of the impact of environmental sensing across the spectrum of enteric endothelia, demonstrating that endothelial AHR signalling integrates dietary cues to maintain tissue homeostasis by promoting EC quiescence and normalcy.
Plasma sKL and Nrf2 Levels in Patients with Calcium Oxalate Stones
Authors: aihemaitijiang baihetiyaer , qi yang , mahemuti mulati , Liu ruotian 
Published: April 2023

Abstract
Objective To investigate the relationship between the plasma levels of sKL and Nrf2 and calcium oxalate calculi.Methods The clinical data of 135 patients with calcium oxalate calculi treated in the Department of Urology of the Second Affiliated Hospital of Xinjiang Medical University from February 2019 to December 2022 (the stone group) and 125 healthy persons who underwent physical examination in the same period (the healthy control group) were collected. The levels of sKL and Nrf2 were measured by ELISA. A correlation test was used to analyze the risk factors for calcium oxalate stones. Logistic regression analysis was used to analyze the risk factors for calcium oxalate stones, and an ROC curve was used to evaluate the sensitivity and specificity of sKL and Nrf2 in predicting urinary calculi.Results Compared with the healthy control group, the plasma sKL level in the stone group was decreased (111.53 ± 27.89 vs. 130.68 ± 32.51), and the plasma Nrf2 level was increased (300.74 ± 114.31 vs. 246.74 ± 108.22). There was no significant difference in the distribution of age and sex between the healthy control group and the stone group, but there were significant differences in plasma levels of WBC, NEUT, CRP, BUN, BUA, and SCr, BMI and eating habits. The results of the correlation test showed that the level of plasma Nrf2 was positively correlated with SCr (r = 0.181, P < 0.05) and NEUT (r = 0.144 P < 0.05). Plasma sKL was not significantly correlated with Nrf2 (r = 0.047, P > 0.05), WBC (r = 0.108, P > 0.05), CRP (r=-0.022, P > 0.05), BUN (r=-0.115, P > 0.05), BUA (r=-0.139, P > 0.05), SCr (r = 0.049, P > 0.05), or NEUT (r = 0.027, P > 0.05). Plasma Nrf2 was not significantly correlated with WBC (r = 0.097, P > 0.05), CRP (r = 0.045, P > 0.05), BUN (r = 0.122, P > 0.05), or BUA (r = 0.122, P > 0.05).=0.078, P > 0.05). Logistic regression showed that elevated plasma sKL (OR = 0.978, 95% CI: 0.969 ~ 0.988, P < 0.05) was a protective factor for the occurrence of calcium oxalate stones. BMI (OR = 1.122, 95% CI: 1.045 ~ 1.206, P < 0.05), dietary habit score (OR = 1.571, 95% CI: 1.221 ~ 2.020, P < 0.05), WBC (OR = 1.551, 95% CI: 1.423 ~ 1.424, P < 0.05), NEUT (OR = 1.539, 95% CI: 1.391 ~ 1.395, P < 0.05) and CRP (OR = 1.118, 95% CI: 1.066 ~ 1.098, P < 0.05) were risk factors for the occurrence of calcium oxalate stones.Conclusion Plasma sKL levels were decreased and Nrf2 levels were increased in patients with calcium oxalate calculi. Plasma sKL may play an antioxidant role in the pathogenesis of calcium oxalate stones through the Nrf2 antioxidant pathway.
Salicylic acid promoted apple metabolic responses against Penicillium expansum infection
Authors: Zhang Jianyi , Ma Ning , Xu Guofeng , Kuang Lixue , Li Zhiyuan , Shen Youming 
Published: April 2023

Abstract
Blue mold caused by Penicillium expansum (P. expansum) infection results in severe postharvest deterioration of apples. Salicylic acid (SA) is an effective elicitor that promotes fruit resistance. However, the metabolic mechanism of P. expansum infection of apples and the SA-mediated metabolic responses are still unknown. In this study, the metabolic changes during apple P. expansum infection and SA-mediated disease resistance were explored by performing ultra-performance liquid chromatography and quadrupole-time of flight mass spectrometry. A total of 472 different metabolites were identified between samples, and the correlated metabolic pathways were revealed by bioinformatics analysis. The upregulation of the tricarboxylic acid (TCA) cycle, galactose metabolism, and starch and sucrose metabolism reflected energy conversion for P. expansum invasion and fruit disease resistance. Changes in glyoxylate and dicarboxylate metabolism and carbapenem biosynthesis reflected the biosynthesis of virulence factors and secondary metabolites for fungal infection. Metabolic pathways related to apple natural disease resistance mainly included the upregulation of secondary metabolite biosynthesis and sphingolipid metabolism. SA promoted the TCA cycle, reactive oxygen metabolism and secondary metabolite biosynthesis of apples for disease resistance. This study improved the understanding of the pathogenic mechanism of P. expansum infection of apples and the metabolic processes for SA-mediated disease resistance.
Sociodemographic and clinical determinants of asymptomatic Plasmodium falciparum infection among School Children in Low Transmission Season in The Kassena Nankana West District of Ghana
Authors: Ashley Jephthah Boateng , Antwi Maxwell Hubert , Kwakye Aaron Siaw , Darban Isaac , Buckman Tonnies Abeku , Akondoe Angela Yawa , Amissah Tawfeek Ahmed , Kuwornu Thomas Kwame 
Published: April 2023

Abstract
Background: Asymptomatic malaria remains a diagnostic challenge and serves as a reservoir forinfection transmission and leads to malaria in children. This study was therefore conducted to assess the sociodemographic and clinical determinants of asymptomatic Plasmodium falciparum (Pf) infection among school children in low transmission season.
Methods:In a cross-sectional study, 328 school children ages 6-14 years were recruited in the study. Asymptomatic malaria status was determined using three laboratory diagnostic method: Rapid Diagnostic Test (RDT), Microscopy and Polymerase Chain Reaction (PCR) analysis. Anaemia as one of the clinical determinants of asymptomatic Plasmodium falciparum infection was diagnosed as low haemoglobin concentration using a haemoglobinometer.
Results: The prevalence of asymptomatic Plasmodium falciparum infection was 13.4%. The mean age was (10.6 ± 2.4) years with majority (52.7%) being in the age category ≥11 years. Most of the participants were females (59.8%). Mean haemoglobin concentration was (10.4 ± 1.8) g/dl. Anaemia was recorded in 91 out of the 328 children, accounting for 27.7% of the studied subjects and was significantly associated with P. falciparum infection [OR = 19.9, 95%CI = (2.7 – 147.2), p = 0.003]. Of the 91 asymptomatic subjects who had anemia, 59 (64.8%) were anaemic with falciparum infection with 29 (31.9%) and 13 (14.3%) having moderate (8-10.9g/dl) and severe (<8g/dl) anaemia with P. falciparum infection respectively. Though statistically insignificant, males were associated with increased odds of malaria infection [OR = 1.8, 95%CI = 0.9 – 3.3), p = 0.083], and the use of mosquito nets as one of the interventive measures against the malaria vector were statistically not significant (p = 0.960) despite low 24.7% of the participants’ patronage.
Conclusion: Prevalence of asymptomatic malaria P. falciparum infection was relatively high and was associated with moderate anaemia in the cohort suggesting that they may be a target of control interventions.
The Impact of Protein Corona Formation on PEGylated NT3- BDNF Nanoparticles - Distribution, Protein Release, and Cytotoxicity in Human Retinal Pigmented Epithelial Cell Model
Authors: Dąbkowska Maria , Kosiorowska Alicja , Machaliński Bogusław 
Published: April 2023

Abstract
The adsorption of biomolecules on the nanoparticles’ surface ultimately depends on the intermolecular forces, which dictate the mutual interaction transforming their physical, chemical, and biological characteristics. Therefore, a better understanding of the adsorption of serum proteins and their impact on nanoparticle physicochemical properties is of utmost importance for developing nanoparticle-based therapies.
We investigated the interactions between potentially therapeutic proteins: neurotrophin 3 (NT3), brain-derived neurotrophic factor (BDNF), and polyethylene glycol (PEG) in a cell-free system and a retinal pigmented epithelium cell line (ARPE-19). The variance of the physicochemical properties of PEGylated NT3-BDNF nanoparticles (NPs) in serum-abundant and serum-free systems were studied using transmission electron microscopy, atomic force microscopy, multi-angle dynamic, and electrophoretic light scattering. Next, we compared the cellular response of ARPE-19 cells after exposure to PEGylated NT3-BDNF NPs in either serum-free or complex serum environment by investigating protein release and cell cytotoxicity using ultracentrifuge, fluorescence spectroscopy and confocal microscopy.
After serum exposure, the decrease in the aggregation of PEGylated NT3-BDNF NPs was accompanied by increased cell viability and BDNF/NT3 in vitro release. In contrast, in a serum-free environment, the appearance of positively charged NPs with hydrodynamic diameters up to 900 nm correlated with higher cytotoxicity, and limited BDNF/NT3 release into the cell culture media.
Our study provides new insights into the role of protein corona when considering the PEGylated nano-bio interface with implications for cytotoxicity, NP’s distribution, and BDNF, NT3 release profiles in the in vitro setting.
A prospective randomized controlled trial measuring satisfaction and parents stress after GOMCO and Plastibell infant circumcision
Authors: Alsowayan Ossamah Saleh , Zahrani Abdullah Mousa Al , Basalelah Jumanah Hamad , Madi Maha Khalid Al , Humam Abdullah Abdlz. Al , Otaibi Aali Naif Al , AlKhamis Abdulmalik Abdulaziz , Fadaak Kamel Hussien , Suhaibani Shaheed Saleh Al , Darawany Hamed Mohamed El 
Published: April 2023

Abstract
Purpose:
To compare the physician and parental satisfactions between the two types of circumcision maneuvers used in our units, GOMCO and Plastibell circumcision.
Methodology:
This study was performed from 2019 to 2021. A total of 190 children were circumcised either by Gomco or plastibell procedure. Each child was evaluated during the procedure, immediately after the operation, and 1, 2, and 3 weeks after the procedure. Data were collected, statistically analyzed using SPSS version 25.
Results:
A total of 190 children underwent circumcision from 2019 to 2021. 98/190 children (51.6%) underwent circumcision by GOMCO, while 92/190 (48.4%) underwent circumcision by Plastibell. Bleeding was significantly higher in plastibell method, while excess skin was significantly higher in GOMCO method (p-value 0.048). Physician evaluation results show no significant difference between both methods in late physician evaluation. 23/92 (28%) of parents were not satisfied after Plastibell circumcision, compared to 8/98 (8.2%) of parents who were not satisfied with the results of Gomco circumcision (P value 0.002).
Conclusion:
Gomco needs a longer operative time, and Plastibell circumcision is less preferable by parents. There is no significant difference in physician satisfaction between the two procedures, but parents are less satisfied with Plastibell circumcision.
Study on Wear Analysis of Ni-20Al2O3 HVOF Micron Layers Using Artificial Neural Network Technique
Authors: Singh Jashanpreet , Singh Ranvijay , Vasudev Hitesh , Ubaidullah Mohd 
Published: April 2023

Abstract
The paper presents the study of the erosion performance of stainless steel SS316L grade. The micron layers of Ni-20Al2O3 powder were sprayed on the surface SS316L by utilizing a high-velocity oxygen–fuel (HVOF) thermal spraying process. Wear experiments were performed in a slurry pot tester (Ducom TR-41) at different speeds varying from 600 to 1500 rpm. During the experiments, the sand was used to erode the coated surface of SS316L. Multi-sized slurry having a solid concentration lies in the range of 30-60% (by weight) was prepared to perform the wear experiments. A neural network technique was implemented to predict the erosion data by training 75% of the total data. Results show that the microhardness of bare and Ni-20Al2O3 HVOF coated SS316L was tested as 196±21 and 393.32±17 VHN respectively. The current ANN model yielded an R-value that was 0.98871 for training, 0.97209 for validation, and 0.96264 for testing accordingly. It was determined that the total R-value of the model was 0.98153. The ANN’s performance evaluated based on the mean squared error (MSE) was determined as 0.0176, 0.00265, and 0.00334 respectively for the training, validation, and testing.
Long-term 10-Year Comparison of Girls with Congenital Adrenal Hyperplasia Who Underwent Early and Late Feminizing Genitoplasty
Authors: Erginel Basak , Ozdemir Banu , Karadeniz Meltem , Poyrazoğlu Sukran , Keskin Erbug , Soysal Feryal Gun 
Published: April 2023

Abstract
Objectives: The appropriate time to perform feminizing genitoplasty in patients with congenital adrenal hyperplasia (CAH) with the 46, XX genotype is still debatable. The aim of our study was to evaluate the relationship between age at surgery and surgical success in patients who had feminizing genitoplasty due to CAH in our clinic.
Methods: Our clinic retrospectively analyzed 14 patients with CAH and 46, XX genotypes with feminizing genitoplasty (clitoroplasty + vaginoplasty) between 2005 and 2022. The patients were divided into two groups. The mean follow-up time was 10 years.
Results: . Group 1 consisted of seven girls (n = 7/14) who has operations before the age of 2 years (mean 11.7 months). Group 2 consisted of seven girls (n = 7/14) who had operation after the age of 2 years (mean 53 months). There was no statistically significant difference between those operated on before and after two years in terms of anatomical assessments, overall cosmetic results and parent satisfaction except the need for additional intervention (P=0.049). When parent satisfaction was compared with additional surgical intervention, as expected, parents’ satisfaction increased as major surgical intervention decreased. This was statistically significant (p = 0.007). The main source of dissatisfaction was repeated surgery among the parents.
Conclusions: The possibility of this additional surgical intervention increases, and patient satisfaction decreases in patients below the age of 2 years. The corrective surgeries can be deffered until the gender identity of the patient matures and the patient autonomy in deciding whether this surgery should occur.
A novel hybrid wavelength division multiplexing integrated NG PON-VLC systems based TWDM/OCDMA for green smart building applications
Authors: Kumari Meet , Hayal Mohammed R. , Elsayed Ebrahim E. , Nurhidayat Irfan , Shah Mohd Asif 
Published: April 2023

Abstract

Green and smart buildings (SBs) are promising solutions to the fast-growing and ongoing urbanization around the globe and are the composition of huge services. The SB can acquire a tremendous amount of information and perform proper handling of many broadcasting residents accordingly. With the abrupt increase in the high-quality data transmission demands over long reach for the large number of connected residents in SBs, conventional data transmission techniques are inadequate to provide adequate services. Thus, in this paper, to improve the intelligence and reliability of SBs with high overall efficiency, cost-effectiveness, and security, a hybrid passive optical network (PON) and visible light communication (VLC) indoor broadcasting system is proposed. The bidirectional hybrid PON-VLC consists of integrated time and wavelength division multiplexing (TWDM) and wavelength division multiplexing (WDM) with optical code division multiplexing (OCDMA) PON with red green blue employed light emitting diodes (RGB LEDs) based VLC system. Seven OCDMA codes are utilized in the proposed work at a 10/2.5Gbps per channel transmission rate over hybrid fiber-VLC channels to strengthen the system performance. The simulation results show that the modified new zero cross-correlation (MNZCC) code outperforms other codes and provides a faithful 100km fiber length and 5m VLC range for 200 active residents. Moreover, VLC distance can be incremented up to 10m wireless range by increasing the received aperture diameter of 25cm. Also, the numerical analysis, as well as comparative performance, depicts the superiority of PON-VLC under the impact of impairments and noise in fiber-VLC link-based SBs.
Symptom guided multimodal neuroimage fusion patterns in children with attention-deficit/hyperactivity disorder and its potential “brain structure-function-cognition-behavior” pathological pathways
Authors: Feng Yuan , Zhi Dongmei , Zhu Yu , Guo Xiaojie , Luo Xiangsheng , Dang Chen , Liu Lu , Sui Jing , Sun Li 
Published: April 2023

Abstract
The “brain-cognition-behavior” process is an important pathological pathway in children with attention-deficit/hyperactivity disorder (ADHD). Symptom guided multimodal neuroimaging fusion can capture behaviorally relevant and intrinsically linked structural and functional features, which can help to construct a systematic model of the pathology. Analyzing the multimodal neuroimage fusion pattern and exploring how these brain features affect executive function (EF) and leads to behavioral impairment is the focus of this study. Based on gray matter volume (GMV) and fractional amplitude of low frequency fluctuation (fALFF) for 152 ADHD and 102 healthy controls (HC), the total symptom score (TO) was set as a reference to identify co-varying components. Based on the correlation between the identified co-varying components and EF, further mediation analysis was used to explore the relationship between brain image features, EF and clinical symptoms. This study found that the abnormalities of GMV and fALFF in ADHD are mainly located in the default mode network (DMN) and prefrontal-striatal-cerebellar circuits, respectively. GMV in ADHD influences the TO through Metacognition Index, while fALFF in HC mediates the TO through behavior regulation index (BRI). Further analysis revealed that GMV in HC influences fALFF, which further modulates BRI and subsequently affects hyperactivity-impulsivity score. To conclude, structural brain abnormalities in the DMN in ADHD may affect local brain function in the prefrontal-striatal-cerebellar circuit, making it difficult to regulate EF in terms of inhibit, shift, and emotional control, and ultimately leading to hyperactive-impulsive behavior.
Severity assessment in melioidosis pneumonia: what is the most appropriate score or factor?
Authors: Nguyen-Ho Lam , Hoang-Thi Hong-Linh , Le-Thuong Vu , Duong-Minh Ngoc , Dang-Vu Thong , Le-Phuong Mai , Truong-Thien Phu , Tran-Van Ngoc 
Published: April 2023

Abstract
Background and objective
Pneumonia induced by Burkholderia pseudomallei is a common clinical entity of melioidosis. Along with the development of melioidosis rapid tests, prognostic assessment for melioidosis pneumonia (MP) patients at admission is essential. Our study aims to evaluate the validity of the mortality prognostic score for acute melioidosis and pneumonic scores in predicting MP patients.
Methods
A prospective study was conducted from the late 2019 to the early 2022 at the respiratory department of the largest hospital in Southern Vietnam.
Results
Of 66 MP patients, mean age 51.17 ± 11.02, male/female ratio 57/9, and 89.39% presented with acute pulmonary melioidosis. The rate of need for intensive respiratory or vasopressor support (IRVS) was 34.8% and the mortality at discharged time was 25.80%. The areas under ROC curve (AUCs) of PSI, CURB-65, and SMART-COP in predicting the IRVS need were 0.813 (p < 0.001), 0.868 (p < 0.001), and 0.910 (p < 0.001), respectively. The AUCs of PSI, CURB-65, SMART-COP, and the mortality score of acute melioidosis in predicting the death outcome were 0.698 (p = 0.017), 0.797 (p < 0.001), 0.797 (p < 0.001), and 0.663 (p = 0.047), respectively. The sensitivity, specificity, and positive likelihood ratio for CURB-65 score ≥ 2 in predicting the IRVS need and the mortality were 69.57% and 70.59%, 90.70% and 83.67%, 7.48 and 11.53, respectively.
Conclusions
MP could present mild to severe clinical scenario with high mortality among severe MP cases. The simple CURB-65 score could be useful in predicting severe MP.
A Systematic Review of the Literature on the Development of New Concepts from the Perspective of Promoting Patient-Centered Care
Authors: Gupta Atantra Das 
Published: April 2023

Abstract
Background: The concept of the patient as an organization is gaining traction in digital healthcare. This concept places the patient in the driver's position, empowering them to manage their health and wellness and make care decisions. It also encourages healthcare providers to collaborate with patients more closely, allowing for a more proactive, individualized approach to healthcare. In addition, this concept contributes to creating an environment in which patients and providers can utilize data-driven technologies such as AI and machine learning to enhance healthcare outcomes.
Objective: Establishing the concept of patient as an organization in health-care.
Design: Analysis of the concept through different patient attributes & existing concepts.
Data Sources: The literature was searched using electronic databases including PubMed, ScienceDirect, Scopus, Google Scholar, with no date limitation. Any quantitative or qualitative studies published in English, which were focused on patient’s attributes & various concepts were included in the study.
Review methods: Walker and Avant’s strategy for concept analysis was used. The patient attributes and the attributes regarded as concepts are studied.
Results: A total of 40 articles were included. Different attributes and existing concepts like engagement, trust, advocacy, acuity, health literacy. cultural competence, self-efficacy, self-care, resilience and social support are explored.
Attributes like age, gender, medical history, lifestyle factors, socioeconomic status, ethnicity, and culture were studied as antecedents. The consequences of Patient as an Organization were also identified, including those pertaining to care recipients, care providers.
Conclusion: A theoretical definition and a conceptual model of new concept has been developed. The attributes as antecedents, and consequences of the said concept has been identified to script a new paradigm of patient-based healthcare model.