bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antigen presenting subset of СD66b<sup>+</sup>CD16<sup>+</sup>CD33<sup>+</sup>HLA-DR<sup>+</sup> neutrophilic granulocytes in acute osteomyelitis in children: Immunomodulating effects of immunotropic hexapeptide in an <i>in vitro</i> experimental system
Authors: Nesterova I. V. , Chudilova G. A. , Teterin Yu. V. , Chicherev E. A. , Chapurina V. N. , Mitropanova M. N. 
Published: June 2023

Inclusion of neutrophilic granulocytes (NG) in inflammation depends on the expression of receptors providing the functions of NG. Acute osteomyelitis (AOM) occupies a central place among purulentinflammatory diseases in children. AOM purulent-necrotic process proceeds in the bone, bone marrow – the site of hematopoiesis. It is interesting to determine the functionally significant NG subsets, their phenotype in OM and evaluate the effect of immunotropic substances for the correction of dysfunctions. Aim: to specify the variants of changes in quantitative and phenotypic characteristics of CD66b+CD16+CD33+HLA-DR-, CD66b+CD16+CD33+HLA-DR+ NG subsets at AOM in children and evaluate the possibility of their immunomodulation under the influence of hexapeptide (HP) – Arginyl-alpha-Aspartyl-Lysyl-Valyl-Tyrosyl-Arginine in vitro.Peripheral blood (PB) of 24 children 8-15 years old AOM were the study group (SG). The comparison group (CG) – 13 healthy children. HP (10-6 g/L) were incubated with PB SG (60 min, 37 °C) to evaluate the effects (SG1). The number of NG subsets CD66b+CD16+CD33+HLA-DR+, CD66b+CD16+CD33+HLA-DR- (FC500, Beckman Coulter, USA), receptor expression density (MFI), phagocytic activity before and after incubation with HP were determined.The NG subset expressing HLA-DR – 29.9 (18.4-43.6) % CD66b+CD16+CD33+HLA-DR+ was registered in children with AOM. The number of CD66b+CD16+CD33+HLA-DR+ was 1.5 times lower (p > 0.05), of CD66b+CD16+CD33+HLA-DR+ was 1.2 times higher (p > 0.05) than before incubation with of HP. The redistribution of subsets apparently occurs due to the binding of HPs to HLA-DR on the NG membrane. Also MFI HLA-DR was low (p > 0.05); the 1.3-fold increase in MFI CD66b, 1.4-fold decrease in MFI CD16 were revealed (p < 0.05).The study was the first to demonstrate the presence of NG subset of CD66b+CD16+CD33+HLA-DR+ in the PB of children with AOM. Subset of CD66b+CD16+CD33+HLA-DR+NG in AOM indicates the appearance of an activated subset of NG in PB with the properties of APC. The positive influence of HP on the phenotypic characteristics of subsets СD66b+CD16+CD33+HLA-DR-, СD66b+CD16+CD33+HLA-DR+. Restoration of phagocytic function of NGs under the influence of HP is connected with the increase of CD66b expression, which influences the effector function of NGs and decrease of CD16 molecule hyperexpression that stipulates decrease of damaging cytotoxic activity of NGs.
Comparative analysis of the expression of the soluble IL-7 receptor in patients with arthropathy
Authors: Kolerova A. V. , Angelskaya O. A. , Chumasova O. A. , Sizikov A. E. , Shirinsky I. V. , Shirinsky V. S. , Blinova E. A. 
Published: June 2023

Arthropathy is one of the most prevalent diseases, which are based on the destruction and remodeling of cartilage and bone tissue. The inflammation that precedes destruction can be caused by mechanical stress on the joints, or by autoimmune reactions. Recently, IL-7 is considered as one of the key cytokines that promote the production of matrix metalloproteinases, catabolic enzymes, T cell-mediated activation of monocytes, and maturation of osteoclasts. The soluble form of the IL-7 receptor can help prolong the lifespan of IL-7 and thereby it ensures the bioavailability of the cytokine and mediates effect of IL-7 on cells. The aim of this study was to determine the soluble form of the IL-7 receptor (sIL-7R) in the blood plasma of patients with rheumatoid arthritis (RA), osteoarthritis (OA), psoriatic arthritis (PsA) and psoriasis vulgaris (PS), as well as healthy individuals. The RA patients included in the study had moderate to high disease activity according to the DAS28 index. Patients with PsA predominantly had moderate and low disease activity (DAS28) and were characterized by mild to moderate disease severity (PASI). In accordance with the PASI index, patients with PS with mild and severe severity of the disease were included in the study. All patients with OA had a metabolic phenotype that is accompanied by an elevated body mass index.sIL-7R was determined in blood plasma by enzyme-linked immunosorbent assay. It was found that in patients with arthropathy, the level of soluble form of IL-7 was increased relative to healthy individuals, with the exception of the group of patients with PsA. Also, a high concentration of sIL-7R was observed in patients with PS. Analyzing the clinical characteristics of the patients, we found that sIL-7R levels were elevated in RA and PsA patients with high disease activity by DAS28. In addition, positive correlations were found between the concentration of sIL-7R and DAS28 in RA and PsA. In patients with PsA with moderate severity of the disease (PASI), the concentration of sIL-7R was also increased relative to donor's values. On the contrary, in patients with PS, a high level of sIL-7R was noted regardless of the severity of the disease. In patients with OA, no relationship was found between sIL-7R levels and clinical parameters.Thus, an elevated level of sIL-7R in patients with arthropathy may indicate the involvement of IL-7 and its receptor system in the pathogenesis of joint diseases. The IL-7 receptor may become a promising target both in the treatment of joint diseases and other autoimmune diseases, including psoriasis.
Dynamics of T helper subpopulations in the critical period of severe injury in children
Authors: Zakirov R. Sh. , Petrichuk S. V. , Freidlyn E. V. , Kuptsova D. G. , Yanyushkina O. G. , Karaseva O. V. 
Published: June 2023

Severe mechanical injury is one of the main reasons behind children’s disability and mortality. Severe injury induces a complex host immune response to tissue injury, a parallel pro- and anti-inflammatory state, bearing an elevated risk for infectious complications (IC) and/or multiple organ failure (MOF). This study aimed to determine the informative immunological criteria of traumatic injury severity and prognosis outcome in children (severe injury group (SInj, ISS ≥ 16), n = 87; mild/moderate injury group (MInj, ISS < 16), n = 34) based on the assessment of absolute cell count (abs) and percentage of such T helper subpopulations as regulatory T lymphocytes – CD4+CD127lowCD25high(Treg), Th17 lymphocytes – CD4+CD161+ and CD4+CD127higtCD25high T cells(T127hi) in severe injury cases grouped by the outcome (favorable, n = 47; unfavorable, n = 40) and depending on IC (n = 16) and the development of MOF (n = 11) on the 1st, 3d , 5th, 7th, 14th day after injury. The control group was comprised of 80 apparently healthy children comparable in age and sex. An inverse relationship between severity of injury, degree of blood loss and outcome of injury was revealed with the abs of all Th populations, but for Th abs and Treg abs the most significant correlation was found (Spearman’s R ≤ -0,70, p < 0.00001). For SInj group, a pronounced decrease of Th abs, Treg abs, T127hi abs and Th17 abs, in the acute post-traumatic period with an increase to 14 days was revealed. The values of in the first day for indicators of patients with MInj group correspond to the values of control group and significantly differ from SInj group. There are different kinetics of percentage Th subpopulations in peripheral blood of children with severe injuries. The Th17%CD4+ and T127hi%CD4+ significant increase in 1st-3d and 3d -7th days after injury respectively in comparation with сontrol and MInj groups. There were no differences between groups in terms of Treg%CD4+. The lower-level Treg abs in trauma patients admitted to the ICU is significantly associated with develop the infectious complications and outcome of trauma. The Th17 abs is significantly reduced in 3-7th days after the injury in the SInj group with MOF. The results of the study indicate that in children levels of Treg, T127hi and Th17 is significantly associated with severity of injury and may be used to predict outcome of trauma and assess the risk of IC and MOF.
Phenotype characteristic of colonic intraepithelial lymphocytes in patients with Crohn's disease
Authors: Nizheharodava D. B. , Shuleika A. Ch. , Starastsin A. M. , Vanslau M. I. , Ivanchyk G. I. , Vialichka A. V. , Zafranskaya M. M. 
Published: June 2023

Intraepithelial lymphocytes (IEL) play a critical role in maintaining the immune balance of the gut and provide the first line of mucosal defense against luminal antigens as well as rapidly respond to epithelial injury. Recently, IEL have received a lot of attention as key mediators of aberrant immune response resulted in persistent immune activation, inflammation and altered intestinal barrier function, seen in Crohn's disease (CD). This study describes for the first time subsets of colonic IEL in CD patients as compared to healthy controls aimed at characterization of altered IEL contribution to the pathogenesis of Crohn's disease.The peripheral venous blood and colon tissues were obtained from 10 CD patients and 6 donors. IEL were isolated from the mucosa by incubation the tissue in a predigesting solution. Lymphoid cells phenotype was investigated using monoclonal antibodies and flow cytometry.The majority of colonic IEL was identified as СD3+T lymphocytes and no significant differences were found in their numbers in investigated groups. However, changes in T cell subsets composition have been shown: the ratio of СD3+СD4+IEL and СD3+СD8+IEL was 1:1 in colon of CD patients and correlated with T cells in peripheral blood (R = 0.7; p < 0.05) while donor tissues were characterized by expected СD3+СD8+T killers prevalence and the ratio reached 1:2 (p < 0.05). The increase of unconventional γδIEL (mainly due to V81+T cells) and СD161+T cells in association with TNK cells decrease were revealed in colon (p < 0.01) as well as in peripheral blood (p < 0.05) of CD patients as compared to donors. Moreover, the number of colonic γδIEL was correlated with disease location (R = -0.6; p < 0.05), and disease behavior (R = 0.7; p < 0.01) according to Montreal classification.The observed data indicates changes in colonic IEL composition in CD patients that may provide valuable insight into the contribution of T helpers, γδT cells and mucosa-associated СD161+T cells in autoimmune intestinal inflammation but need further possible mechanisms discussion.
Efficacy of Yoga Nidra on Depression, Anxiety, and Insomnia in Frontline COVID-19 Healthcare Workers: A Pilot Randomized Controlled Trial
Authors: Gunjiganvi Mallikarjun , Rai Siddharth , Awale Rupali , Mishra Prabhaker , Gupta Devendra , Gurjar Mohan 
Published: June 2023

Abstract
The COVID-19 pandemic has led to a surge of mental health disturbances and the subsequent use of various mind-body therapies. Although evidence supports the benefits of yoga for mental health in a variety of disease states, information on its effects among healthcare workers during the COVID-19 epidemic is scarce. Therefore, this study evaluated and compared the efficacy of relaxation to music and yoga nidra on the mental health of frontline healthcare workers during the pandemic. This open-label randomized trial was conducted at a Level III COVID-19 care center. In the Relaxation-to-Music Group, participants received deep relaxation music, whereas those in the Yoga Nidra Group performed yoga nidra practices; both interventions were delivered through a YouTube platform and were to be done daily for 30 minutes during the healthcare workers’ 2-week duty periods. The primary outcomes were measured using scores of the Patient Health Questionnaire (PHQ)–9, Generalized Anxiety Disorder (GAD)–7 scale, and Insomnia Severity Index (ISI) at the end of the duty period. A total of 79 healthcare workers were randomly divided into two groups: (1) Relaxation-to-Music (n = 40) and (2) Yoga Nidra (n = 39). Demographics; clinical characteristics; and PHQ-9, GAD-7, and ISI scores of the two groups were comparable at baseline. In the Yoga Nidra Group, PHQ-9 scores decreased significantly (5.17 ± 4.25 to 3.03 ± 2.40, p = 0.002) compared to the Relaxation-to-Music Group (5.68 ± 4.73 to 4.34 ± 2.90, p = 0.064). Similarly, GAD-7 scores decreased significantly in the Yoga Nidra Group (4.93 ± 3.27 to 2.33 ± 2.56, p &lt; 0.001) compared to the Relaxation-to-Music Group (4.84 ± 3.94 to 4.03 ± 3.56, p = 0.123). ISI scores also decreased significantly in the Yoga Nidra Group (6.10 ± 3.53 to 3.03 ± 2.88, p &lt; 0.001) compared to the Relaxation-to-Music Group (6.09 ± 5.37 to 5.93 ± 5.95, p = 0.828). In this study, yoga nidra practice was more helpful than relaxation to music in reducing depression, anxiety, and insomnia among frontline COVID-19 healthcare workers during their duty periods.
Doctrinal Approaches to Understanding Evidence in a Criminal Case: History and Modernity
Authors: Rossinskiy S. B. 
Published: June 2023

The paper discusses the main stages of the development of the theory of criminal procedural evidence. The author analyzes and systematizes various approaches of procedural researchers, on the basis of which the basic doctrinal approaches to understanding evidence are distinguished: 1) formal-logical; 2) factual; 3) dualistic; 4) liminal (transitional); 5) synthetic; 6) cybernetic (informational); 6) pragmatic (Anglo-Saxon). The paper defines the causes of their emergence, evaluates their advantages and disadvantages.It is noted that the diversity of approaches to understanding evidence can be explained by the predetermination of various academic approachers and related legislative provisions, initially different ideological ideas and trends that play a priority role in certain periods of society development. At the same time, it is argued that these approaches do not exclude each other, but reveal different elements of criminal procedural evidence as a comprehended, multidimensional complex of cognitive-certifying techniques and argumentative-logical operations: the cybernetic approach as accumulation of useful information; pragmatic approach as its proper certification; formal-logical and factual approaches as justification of law enforcement decisions and positions of the parties, etc.In this regard, the conclusion is formulated about the optimality and the greatest acceptability for the use of another, so-called non-synthetic approach. Evidence is proposed to be understood as information assets provided for by law and predetermined by substantive or ideal traces-displays, subject to accumulation as legally suitable (permissible) information products, and then used as argumentative resources, which support the investigator, inquiror, court in establishing circumstances relevant to the criminal case and substantiating law enforcement decisions., and to the parties in attempts to influence the nature and content of these decisions.
Evaluation of skeletal changes in glenoid fossa, condylar head and articular space following fixed functional appliance therapy using cone beam computed tomography – A clinical prospective study
Authors: Agrawal Kunaal , Shashikala Kumari V.  
Published: June 2023

This study was conducted to evaluate skeletal changes in glenoid fossa, condylar head and articular space following Powerscope appliance therapy using CBCT.This study included twenty patients (age group of 11-15 years) having Class II Division 1 skeletal & dental malocclusion. A pre-treatment CBCT scan (T1) of both TMJs was taken. Following leveling & aligning, Powerscope appliance was inserted, which was sequentially activated using crimpable shims until edge-to-edge bite was obtained. After completion of phase 1 of treatment, another CBCT scan (T2) was taken. Condylar position, length, height, glenoid fossa & articular space measurements were obtained from pre- & post-treatment CBCT scans and data obtained were statistically analysed and compared by Paired-t test.Comparison between T1 & T2 data showed that the condyles were shifted anteriorly by 1.08 mm, condylar length and height increased by 0.26 mm and 0.71 mm, respectively. Glenoid fossa and anterior articular space decreased by 1.50⁰ and 0.23 mm, whereas, middle and posterior articular spaces increased linearly by 2.55 mm and 1.85 mm, respectively.Powerscope shifted the condyle anteriorly, increased its length and height, and reduced the glenoid fossa angle. Anterior articular space decreased whereas middle and posterior articular spaces increased.
To evaluate and compare surface characteristics of different aesthetic nickel-titanium archwires using surface profilometry, sem and stereomicroscopy: A multi-arm split-mouth randomized controlled trial
Authors: Sharma Madhur , Kumar Mukesh , Goyal Manish , Abrar Md , Shaha Kalpit , Kumar Sumit 
Published: June 2023

This multi-arm split-mouth randomized controlled trial aimed to compare the surface characteristics, coating thickness and coating stability of four different aesthetic coated Nickel-Titanium Orthodontic rectangular archwires before and after oral exposure. To evaluate and compare the surface characteristics of four as-received (Control group) aesthetic nickel-titanium orthodontic rectangular archwires (0.016 x 0.022ʺ NiTi) from different manufacturers that are BioForce (Group I), Rabbit force (Group II), Libral (Group III), and ORMCO (Group IV) and after oral exposure (Experimental group) of four weeks, 15 patients meeting the inclusion criteria were recruited for this prospective study. Scanning electron microscopy, stereomicroscopy, and 3D profilometry were done for evaluating the surface characteristics of each group of wires.Four quadrants of every patient were randomized in a 1:1:1:1 ratio to the wires of either BioForce (Group I), Rabbit force (Group II), Libral (Group III), or ORMCO (Group IV). On examination of as-received wires (control group), the maximum coating thickness was seen in Group III c (Teflon coated, Libral), followed by Group IIc (Epoxy coated, Rabbit Force), then Group Ic (Teflon coated, BioForce), and least in Group IVc (Epoxy coated, ORMCO). After 4 weeks of oral exposure, the maximum coating loss was seen in Group II (Rabbit Force) wire, and the least amount of coating loss was seen in Group IV (ORMCO).The results of this study indicated a low aesthetic value of each of the manufacturer’s coated archwires because they presented a non-durable coating after oral exposure.
Assessment of changes in maxillary dentoalveolar complex following retraction and intrusion of maxillary anterior teeth in mixed Indian population
Authors: Andhare Pushkar , Datana Sanjeev , Agarwal Shiv , Chopra SS , Roy ID 
Published: June 2023

The biology of tooth movement implies to selective resorption and deposition of the alveolar bone during orthodontic tooth movement. The controversy about "tooth movement through bone" and "tooth movement with bone” is still not settled. The cortices of the alveolar bone are considered boundaries of the alveolar complex that limit the orthodontic tooth movement. The present study evaluated the change in these limits of maxillary dentoalveolar complex post-retraction and intrusion of maxillary anterior teeth.: CBCT data of 15 subjects was assessed to primarily measure the changes in thickness, area and volume of anterior maxillary alveolar bone following retraction & intrusion. As a secondary objective, tooth length was also assessed to ascertain external apical root resorption, post-retraction and intrusion of maxillary anterior teeth. A statistically significant reduction in alveolar bone thickness, mean area and volume on the palatal side was evident and also reduction in tooth length of maxillary central and lateral incisor was observed post retraction and intrusion of maxillary anterior teeth (P-value&#60;0.05). It is essential to evaluate the alveolar housing prior to considering retraction and intrusion of anterior teeth in order to plan appropriate biomechanics and treatment protocol which would minimize iatrogenic side effects and yield a stable treatment outcome.
Evaluation of Some Biochemical Parameters of COVID-19 Patients as Biomarkers
Authors: KARADENİZ Ümit , UĞRAŞ Serpil , GÖÇ RASGELE Pınar 
Published: June 2023

Objective: SARS-CoV-2 has caused a global pandemic with more than 664 million confirmed cases and more than 6.7 million deaths worldwide. In pandemic, several studies have been done about the relationship of some biochemical and hematological parameters with COVID 19 in order to assist diagnosis and treatment. However, a lot of information is not completely complete and there are contradictions in the data. In the present study, it was focused to compare the biochemical parameters of patients and healthy individuals due to COVID 19 by investigating the relationship between age and gender.
Methods: The 317 individuals who consulted to the Pandemic Outpatient Clinic with the suspicion of COVID 19 between February 2022 and November 2022 were evaluated by laboratory tests. In present study, biochemical parameter values of individuals with positive and negative COVID 19 test outcomes were used as material. These biochemical parameters were compared statistically based on different age ranges and gender groups.
Results: It was ascertained that the values such as UREA, KREA, FER, AST, ALT, HSTI, CK and CRP in male patients with COVID 19 were higher than female patients with COVID 19. Moreover, it was detected that there was a statistically significant increase in parameters such as GLU, FER, AST, LDH, HSTI, CK and CRP in COVID 19 patients aged 18-65 years.
Conclusions: Many biomarkers of prognostic importance are expensive,	ordinary and pricey-effective biomarkers such as FER, AST, LDH, HSTI, CK and CRP can be used to monitor disease in patients with COVID 19 infection.
НАУКОВО-МЕТОДИЧНИЙ ПІДХІД ДО РОЗПОДІЛУ ЕКСПЛУАТАЦІЙНИХ ВИТРАТ НА УТРИМАННЯ ПІДПРИЄМСТВ ЗАЛІЗНИЧНОГО ТРАНСПОРТУ ЗА УМОВИ ЗБИТКОВОСТІ
Authors: Калабухін Ю. Є. , Зоріна О. І. , Каменева Н. М. 
Published: June 2023

Однією з важливіших складових визначення ефективності залізничних станцій, у тому числі і малодіяльних, є рівень експлуатаційних витрат для здійснення виробничо-господарської діяльності. Сучасні особливості обліку експлуатаційних витрат вантажних станцій ускладнюють достовірність оцінювання ефективності роботи кожної з них. В статті запропоновано науково-методичний підхід до розподілу фактичних експлуатаційних витрат на утримання вантажних станцій регіональної філії. Даний підхід дозволяє розподілити фактичні експлуатаційні витрати відповідних господарств регіональної філії на утримання підпорядкованих вантажних станцій для вирішення проблеми визначення ефективності їх виробничо-господарської діяльності.