bluuebunny/crossref_metadata_2025_split huggingface dataset:
Augmenter of liver regeneration promotes drug resistance of acute lymphoblastic leukemia through the alteration of mitochondrial functions and the inhibition of the mitochondrial apoptosis pathway
Authors: Zhou Jingwen , Zhou Ying , Yuan Shiyi , Li Yifei , Xiao Wenrui , Zhang Ping , Lou Shifeng , Shen Yan 
Published: June 2023

AbstractBackgroundAcute T lymphoblastic leukemia (T‐ALL) occurs in 25% of adults diagnosed with Acute lymphocytic leukemia (ALL), and drug resistance is still a clinical obstacle. Augmenter of liver regeneration (ALR) is important to ALL drug resistance and is involved in the regulation of mitochondrial function; we speculated that the high expression of ALR in T‐ALL promotes drug resistance through the alteration of mitochondrial function and the inhibition of the mitochondrial apoptosis pathway.MethodWe silenced and overexpressed ALR in the T‐ALL cell lines that were untreated or treated with dexamethasone (DXM) or methotrexate (MTX). Apoptosis, proliferation, reactive oxygen species and ATP productions, mitochondrial membrane potential, and mitochondrial respiratory chain complex expression in cells were examined. The data were collated to comprehensively evaluate the effects of ALR expression change on mitochondrial function and drug resistance in T‐ALL cells.ResultsALR knockdown led to the inhibition of proliferation, an increase in apoptosis, and the promotion of the cells' sensitivity to drugs. It also showed mitochondrial dysfunction. ALR knockdown actived the mitochondrial apoptosis pathway. The treatment of ALR knockdown T‐ALL cells with MTX or DXM further altered the mitochondrial function of T‐ALL cells and actived the mitochondrial apoptosis pathway. Overexpression of ALR promoted cell proliferation and drug resistance, reduced apoptosis, protected mitochondrial function, and inhibited the mitochondrial apoptosis pathway.ConclusionT‐ALL resistance caused by ALR through the alteration of mitochondrial function is associated with the inhibition of the mitochondrial apoptosis pathway.
Comprehensive essentiality analysis of the
Mycobacterium kansasii
genome by saturation transposon mutagenesis and deep sequencing
Authors: Levendosky Keith , Janisch Niklas , Quadri Luis E. N. 
Published: June 2023

ABSTRACT

Mycobacterium kansasii
(
Mk
) is an opportunistic pathogen that is frequently isolated from urban water systems, posing a health risk to susceptible individuals. Despite its ability to cause tuberculosis-like pulmonary disease, very few studies have probed the genetics of this opportunistic pathogen. Here, we report a comprehensive essentiality analysis of the
Mk
genome. Deep sequencing of a high-density library of
Mk Himar1
transposon mutants revealed that 86.8% of the chromosomal thymine–adenine (TA) dinucleotide target sites were permissive to insertion, leaving 13.2% TA sites unoccupied. Our analysis identified 394 of the 5,350 annotated open reading frames (ORFs) as essential. The majority of these essential ORFs (84.8%) share essential mutual orthologs with
Mycobacterium tuberculosis
(
Mtb
). A comparative genomics analysis identified 139
Mk
essential ORFs that share essential orthologs in four other species of mycobacteria. Thirteen
Mk
essential ORFs share orthologs in all four species that were identified as being not essential, while only two
Mk
essential ORFs are absent in all species compared. We used the essentiality data and a comparative genomics analysis reported here to highlight differences in essentiality between candidate
Mtb
drug targets and the corresponding
Mk
orthologs. Our findings suggest that the
Mk
genome encodes redundant or additional pathways that may confound validation of potential
Mtb
drugs and drug target candidates against the opportunistic pathogen. Additionally, we identified 57 intergenic regions containing four or more consecutive unoccupied TA sites. A disproportionally large number of these regions were located upstream of
pe
/
ppe
genes. Finally, we present an essentiality and orthology analysis of the
Mk
pRAW-like plasmid, pMK1248.

IMPORTANCE

Mk
is one of the most common nontuberculous mycobacterial pathogens associated with tuberculosis-like pulmonary disease. Drug resistance emergence is a threat to the control of
Mk
infections, which already requires long-term, multidrug courses. A comprehensive understanding of
Mk
biology is critical to facilitate the development of new and more efficacious therapeutics against
Mk
. We combined transposon-based mutagenesis with analysis of insertion site identification data to uncover genes and other genomic regions required for
Mk
growth. We also compared the gene essentiality data set of
Mk
to those available for several other mycobacteria. This analysis highlighted key similarities and differences in the biology of
Mk
compared to these other species. Altogether, the genome-wide essentiality information generated and the results of the cross-species comparative genomics analysis represent valuable resources to assist the process of identifying and prioritizing potential
Mk
drug target candidates and to guide future studies on
Mk
biology.
Short-Term Pretreatment with Melatonin Increases the Resistance of Barley Plants to the Subsequent Action of Cadmium
Authors: Danilova E. D. , Zlobin I. E. , Efimova M. V. 
Published: June 2023

Abstract
The effect of short-term and long-term root treatment with melatonin on growth parameters, photochemical activity of PSII, content of basic photosynthetic pigments, lipid peroxidation, and accumulation of inorganic ions in barley plants under the action of cadmium chloride was studied. It was shown for the first time that the protective effect of phytomelatonin during short-term treatment is manifested in a decrease in the toxic effect of cadmium on the content of photosynthetic pigments and the accumulation of cadmium ions in the shoots and roots of barley plants. The protective effect of melatonin is based on its ability to reduce the intensity of oxidative stress by maintaining the level of carotenoids and increasing the activity of antioxidant enzymes. The effectiveness of short-term use of melatonin in a number of indicators exceeds its protective effect from long-term exposure. The data obtained indicate the possibility of using melatonin as a substance that induces the state of plant priming upon subsequent exposure to cadmium.
Risk factors for irinotecan-induced liver injury: a retrospective multicentre cross-sectional study in China
Authors: Han Jun , Liu Jianhua , Yu Zaoqin , Huang Rui , Zhao Li , Xu Yi , Chen Min , He Guangzhao , Song Qiuyan , Li Wei , Zhang Chengliang 
Published: June 2023

ObjectivesThe hepatotoxicity of irinotecan has been widely implicated in the treatment of multiple solid tumours. However, there are few studies on the influencing factors of irinotecan-induced hepatotoxicity. Herein, we investigated the risk factors for irinotecan-induced liver injury among 421 patients receiving irinotecan-based regimens (IBRs).DesignRetrospective multi-centre cross-sectional study.SettingThis study surveyed four hospitals in China.ParticipantsAfter excluding participants with missing variables, we retrospectively collected the demographic, clinical and therapeutic data of 421 patients who received IBRs in four hospitals between January 2020 and December 2021 and divided the patients into two groups: those without liver injury and those with liver injury.ResultsThe 421 enrolled patients were grouped (liver injury group: n=92; control group: n=329) according to their hepatic biochemical monitoring parameters. In our study, the multivariate logistic regression results showed that three to four cycles of chemotherapy (OR (95% CI): 2.179 (1.272 to 3.733); p=0.005) and liver metastasis (OR (95% CI): 1.748 (1.079 to 2.833); p=0.023) were independent risk factors for irinotecan-induced liver injury. The Cox proportional hazards model demonstrated that alcohol consumption history (OR (95% CI): 2.032 (1.183 to 3.491); p=0.010) and a cumulative dose of irinotecan ≥1000 mg (OR (95% CI): 0.362 (0.165 to 0.792); p=0.011) were significantly correlated with the onset time of irinotecan-induced liver injury.ConclusionsThese findings suggest that patients with liver metastasis or who received three to four cycles of chemotherapy should undergo rigorous liver function monitoring to prevent or reduce the incidence of irinotecan-induced liver injury. Moreover, patients with a history of alcohol consumption should also be closely monitored.
Emotional freedom technique versus written exposure therapy versus waiting list for post-traumatic stress disorder: protocol for a randomised clinical MRI study
Authors: Choi Yujin , Kim Yunna , Choi Sunyoung , Choi Young-Eun , Kwon Ojin , Kwon Do-Hyung , Lee Seung-Ho , Cho Seung-Hun , Kim Hyungjun 
Published: June 2023

IntroductionThe emotional freedom technique (EFT) is an acupuncture-based psychotherapy that combines tapping on acupoints with cognitive reframing. EFT has been previously shown to have potential for treating post-traumatic stress disorder (PTSD). However, further clinical evidence and underlying mechanisms of EFT are yet to be fully explored. This proposed clinical trial aims to examine the effect of EFT on patients with PTSD compared with the waitlist (WL) and active controls.Methods and analysisThis study was designed as a randomised, assessor-blinded, three-arm clinical MRI study. A total of 120 eligible patients with PTSD will be recruited and randomised into EFT, written exposure therapy (WET) or WL groups. EFT and WET will be applied once a week for 5 weeks. For patients in the WL group, EFT will be performed after 12 weeks. PTSD symptoms, depression, anxiety, somatic symptoms and quality of life will be evaluated. Assessments will be conducted at baseline (week 0), post-treatment (week 6) and follow-up (week 12). Structural and functional brain images and recording videos of facial expressions to emotional stimuli will be obtained before and after treatment. Sixty participants without lifetime traumatic experiences will be enrolled as healthy controls. The primary objective of the study is to compare the change from baseline in the Clinician-Administered PTSD Scale after treatment (week 6) between EFT and WL groups and between EFT and WET groups.Ethics and disseminationEthical approval was obtained from the Institutional Review Board of the Kyung Hee University Korean Medicine Hospital. The research findings will be shared at national and international conferences and will be published in peer-reviewed journals.Trial registration numberClinical Research Information Service KCT0007360https://cris.nih.go.kr/cris/search/detailSearch.do/21974.
Adolescent mental well-being, religion and family activities: a cross-sectional study (Northern Ireland Schools and Wellbeing Study)
Authors: Bamford Jordan , Leavey Gerard , Rosato Michael , Divin Natalie , Breslin Gavin , Corry Dagmar 
Published: June 2023

ObjectivesIn this study, we seek to explore the relationship between adolescent mental well-being, religion and family activities among a school-based adolescent sample from Northern Ireland.SettingThe Northern Ireland Schools and Wellbeing Study is a cross-sectional study (2014–2016) of pupils in Northern Ireland aged 13–18 years.Participants1618 adolescents from eight schools participated in this study.Outcomes measuresOur primary outcome measure was derived using the Warwick-Edinburgh Mental Wellbeing Scale. We used hierarchical linear regression to explore the independent effects of a range of personal/social factors, including religious affiliation, importance of religion and family activities.ResultsIn fully adjusted models, older adolescents and females reported lower mental well-being scores—for the year-on-year increase in age β=−0.45 (95% CI=−0.84, –0.06), and for females (compared with males) β=−5.25 (95% CI=−6.16, –4.33). More affluent adolescents reported better mental well-being. No significant differences in mental well-being scores across religious groups was found: compared with Catholics, Protestant adolescents recorded β=−0.83 (95% CI=−2.17, 0.51), other religious groups β=−2.44 (95% CI=−5.49, 0.62) and atheist adolescents β=−1.01 (95% CI=−2.60, 0.58). The importance of religion in the adolescents’ lives was also tested: (compared with those for whom it was not important) those for whom it was very important had better mental well-being (β=1.63: 95% CI=0.32, 2.95). Higher levels of family activities were associated with higher mental well-being: each unit increase in family activity produced a 1.45% increase in the mental well-being score (β=0.78: 95% CI=0.67, 0.90).ConclusionsThis study indicates that non-religious adolescents may have lower mental well-being scores when compared with their more religious peers, irrespective of religious denomination. This may relate to both a sense of lack of firm identity and perceived marginalisation. Additionally, adolescents with poor family cohesion are more vulnerable to poor mental well-being.
Evaluating the efficacy and safety of neoadjuvant pembrolizumab in patients with stage I–III MMR-deficient colon cancer: a national, multicentre, prospective, single-arm, phase II study protocol
Authors: Justesen Tobias Freyberg , Gögenur Ismail , Tarpgaard Line Schmidt , Pfeiffer Per , Qvortrup Camilla 
Published: June 2023

IntroductionWithin the last two decades, major advances have been made in the surgical approach for patients with colorectal cancer. However, to this day we face considerable challenges in reducing surgery-related complications and improving long-term oncological outcomes. Unprecedented response rates have been achieved in studies investigating immunotherapy in patients with mismatch repair deficient (dMMR) colorectal cancer. This has raised the question of whether neoadjuvant immunotherapy may change the standard of care for localised dMMR colon cancer and pave the way for organ-sparing treatment.Methods and analysisThis is an investigator-initiated, multicentre, prospective, single-arm, phase II study in patients with stage I–III dMMR colon cancer scheduled for intended curative surgery. Eighty-five patients will be treated with one dose of pembrolizumab (4 mg/kg) and within 5 weeks will undergo a re-evaluation with an endoscopy and a CT scan—to assess tumour response—before standard resection of the tumour. The primary endpoint is the number of patients with pathological complete response, and secondary endpoints include safety (number and severity of adverse events) and postoperative surgical complications. In addition, we aspire to identify predictive biomarkers that can point out patients that achieve pathological complete response.Ethics and disseminationThe Regional Committee for Health Research and Ethics and the Danish Medicines Agency have approved this study. The study will be performed according to the Helsinki II declaration. Written informed consent will be obtained from all participants. The results of the study will be submitted to peer-reviewed journals for publication and presented at international congresses.Trial registration numberNCT05662527.
Selected Karoo geoheritage sites of palaeontological significance in South Africa and Lesotho
Authors: Bordy Emese M. , Smith Roger M. H. , Choiniere Jonah N. , Rubidge Bruce S. 
Published: June 2023

Abstract

The main Karoo Basin of South Africa and Lesotho preserves
c.
120 myr of Earth's history. The sedimentary rocks of its Karoo Supergroup record massive environmental changes from the glacial Carboniferous to desert dunes and fiery flood basalts in the Early Jurassic. From the early Permian, the Karoo Basin was gradually filled with fluvial and lacustrine deposits, and the alluvial plains were successively colonized by a diverse suite of plants and animals. The fossils of these ancient inhabitants and their behavioural traces form an astounding Gondwanan geoheritage legacy in southern Africa, providing fossil evidence for the moving lithospheric plates and the effects of four mass extinctions and their subsequent biotic recovery. Here, we present six Karoo sites of global geoscientific importance that best display that heritage, with the caveat that these sites only touch upon the Karoo riches that are available for academic research and the emerging palaeotourism industry. It is our hope that these sites will become anchor points for a sustainable geoheritage future in southern Africa.
Net impact implementation application development life-cycle management in banking sector
Authors: Pramitasari Noviana , Ruldeviyani Yova , Zarkasie Irvan Ramadhan 
Published: June 2023

Digital transformation in the banking sector creates a lot of demand for application development, either new development or application enhancement. Continuous demand for reimagining, revamping, and running applications reliably needs to be supported by collaboration tools. Several big banks in Indonesia use Atlassian products, including Jira, Confluence, Bamboo, Bitbucket, and Crowd, to support strategic company projects. We need to measure the net impact of application development life-cycle management (ADLM) as a collaboration tool. Using the deLone and McLean model, process questionnaire data from banks in Indonesia that use ADLM. Processing data using structural equation modeling (SEM), multiple variables are analyzed statistically to establish, estimate, and test the causation model. The conclusions highlight that system quality strongly affected only User Satisfaction (p=0.049 and β=0.39). Information quality strongly affected use (p=0.001 and β=0.84) and strongly affected user satisfaction (p=0.169 and β=0.28). Service quality strongly affected only use (p=0.127 and β=0.31). Conclusion research verifies the information system's achievement approach described by DeLone and McLean. Importantly, it was discovered that system usability and quality were key indicators of ADLM success. To fulfill their objective, ADLM must be developed in a way that is simple to use, adaptable, and functional.
Ch'ixi Epistemology and The Potosí Principle in the 21st Century
Authors: Alberro Alexander 
Published: June 2023

Abstract
The author focuses on the project exhibition, “The Potosí Principle,” curated by Alice Creischer, Max Hinderer, and Andreas Siekmann Initially installed in Madrid in 2010 and then traveling to Berlin and La Paz, the show cut across the institutionally defined and often rigorously guarded boundaries between curatorial practice, aesthetic expression, and scholarly research to explore global capitalism's dynamics from the perspective of the Spanish colonial empire and its distinctive imagery. However, despite the exhibition's creative installation techniques and revisionist history, it generated a considerable scandal when a self-organized group of La Paz-based artists and scholars committed to anticolonial practices accused the curators of continuing the logic through which the modern West has represented others. The author argues that this criticism parallels the often-fraught negotiations between artists, curators, and curated cultures at the boundary zones between art frameworks.
Can Tomb Terminology Be Used as a Criterion for Dating the Pyramid Texts?
Authors: Hainline Brendan 
Published: June 2023

Abstract
Scholars have long recognized that some Pyramid Text utterances display signs of age that indicate a pre-monumental history of use. Two utterances in particular, PT 355 and PT 662, have been dated by scholars as early as the Pre- or Early Dynastic, based on lines interpreted as describing a pre-pyramidal royal tomb-form (a mudbrick mastaba or a sand grave). This article evaluates the assumptions upon which these claims are based and uses this question to examine the complex interaction between lexical semantics, religious beliefs, and material culture. This article will show that tomb terminology is not a definitive indicator of an early date of composition of Pyramid Text utterances, as the association of lexemes with specific archaeological building-types is problematized by innovations in language, religion, and architecture that occurred in the early Old Kingdom, as well as changes in modern scholarship’s understanding of the context and use of the Pyramid Texts.
Power and Space Production: A Bourdieusian Approach to Environmental Law and Inspection in China
Authors: Ye Liangfei 
Published: June 2023

Abstract
The most important tool or institution that the Chinese state has used since 2002 to address environmental pollution, relying on its power to force compliance by local polluters and cadres, is environmental inspection. This article proposes a Bourdieusian approach for analyzing environmental inspection by considering the symbolic power of the state and the local effects of inspections at different levels of government, which have seldom been explored in the literature. We use this approach to examine four propositions with a sample comprising ninety-nine inspections in city H. First, inspectors use both the objective and symbolic power of the state in inspections. Second, inspectors at lower levels in the bureaucratic hierarchy have less objective and symbolic power in confronting local cadres and polluters. Third, local environmental protection changes physical space (e.g., landscape), social space (e.g., relations between cadres and polluters), and mental space (e.g., environmental perceptions). Fourth, inspectors with more power produce larger spaces for environmental protection. Through using this approach, we offer insights into how the Chinese state uses inspection to mobilize local societies to address social or governance crises.
Historical Events as ‘Magic Yarn Balls’
Authors: Van den Heede Pieter J.B.J. , Polak Sara A. 
Published: June 2023

Abstract
Historical analogies are widely invoked to characterize contemporary events. As we argue that historians should play a central role in assessing these analogies, or at least, that history should offer organized models to think about them, we present a framework for productively engaging with historical analogies in the public debate, policy discussions and elsewhere. First, we introduce the ‘magic yarn ball’ as a central metaphor to capture the textured complexity of historical phenomena in terms of similarity and difference. Second, we propose a general framework inspired by this metaphor to engage with historical analogies, centered on interrogation, individuation, and prospection. We apply our model by assessing analogies between the appointment of Adolf Hitler as chancellor of Germany in 1933 and the inauguration of US president Donald Trump in 2017.