bluuebunny/crossref_metadata_2025_split huggingface dataset:
El marketing ecológico y su incidencia en el desarrollo del ecoturismo en las Lomas de Quilmaná
Authors: Alcalá Sánchez Kiara Fiorella , Bautista Tomairo Nelly Beatriz , Chuquihuamani Chipana Marco Antonio , Flores Guzmán Jerson Daniel , Huamán Payano Raquel , Napanga Yalle Guina Yerica , Peñafiel Huamaní Betssabe Abadesa , Quispe Fernández Liz Andrea , Yactayo Sánchez Richard Alfredo , Yallico Huamán Marinelia Yulisa 
Published: August 2023

El turismo en el Perú ha experimentado un crecimiento sostenible y considerable en los últimos diez años, por la diversidad que tiene este territorio, que va desde su riqueza natural hasta su gastronomía. Las áreas naturales, como las lomas que se encuentran en la costa del Perú, deben contar con un plan de marketing que ayude a contribuir en el desarrollo económico y social de la población aledaña a dicha zona. El objetivo del presente trabajo de investigación es demostrar si el marketing ecológico incide en el desarrollo del ecoturismo en las Lomas de Quilmaná, para poder elaborar un plan de marketing que ayude a incrementar la visita de turistas y genere un desarrollo económico en esta zona. La presente investigación es cuantitativa, siendo la principal fuente de información la encuesta online que se realizó a 50 personas para conocer las opiniones acerca de las Lomas de Quilmaná. También se ejecutó un trabajo de campo, mediante una visita presencial para evaluar el estado en el que se encuentra dicha zona; los resultados muestran el descuido que se tiene en el cuidado de conservación de las Lomas, la falta de señalización, poca difusión por redes sociales, la contaminación, entre otras. Se evidencia que la aplicación del marketing ecológico si incide en el desarrollo del ecoturismo en las Lomas de Quilmaná, a medida que se promocione la zona, el ecoturismo también se irá incrementando. Palabras clave: marketing ecológico, desarrollo, ecoturismo, Lomas de Quilmaná
Manajemen Sarana Dan Prasarana Dalam Meningkatkan Mutu Pembelajaran Taman Kanak-Kanak
Authors: Sandi Qalka , Fauzi Hairul 
Published: August 2023

Manajemen sarana dan prasarana sangat penting untuk dalam meningkatkan mutu pembelajaran anak usia dini. Tujuan penelitian untuk mendeskripsikan manajemen sarana dan prasarana dalam meningkatkan mutu pembelajaran taman kanak-kanak. Metode penelitian yang digunakan yaitu metode deskriptif kualitatif, dengan subjek penelitian yaitu kepala sekolah, wakil kepala sekolah bidang kesiswaan dan kurikulum, bendahara, serta staf tata usaha. Teknik pengumpulan data berupa wawancara, observasi, wawancara dan studi dokumentasi. Teknik analisis data menggunakan analisis deskriptif yakni reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa: (1) Kebijakan perencanaan sarana dan prasarana disusun pada awal tahun dengan menganalisis kebutuhan; (2) Kebijakan pengadaan sarana dan prasarana sesuai dengan prosedur yang ada; (3) kebijakan pengorganisasian sarana dan prasarana sesuai dengan keputusan kepala sekolah; dan (4) pemeliharaan sarana dan prasarana cukup akuntabel
GGA1 participates in spermatogenesis in mice under stress
Authors: Jiao Haoyun , Chen Yinghong , Han Tingting , Pan Qiyu , Gao Fei , Li Guoping 
Published: August 2023

Background
Infertility is recognized as a common and worrisome problem of human reproduction worldwide. Based on previous studies, male factors account for about half of all infertility cases. Exposure to environmental toxicants is an important contributor to male infertility. Bisphenol A (BPA) is the most prominent toxic environmental contaminant worldwide affecting the male reproductive system. BPA can impair the function of the Golgi apparatus which is important in spermatogenesis. GGA1 is known as Golgi-localized, gamma adaptin ear-containing, ARF-binding protein 1. Previously, it has been shown that GGA1 is associated with spermatogenesis in Drosophila, however, its function in mammalian spermatogenesis remains unclear.

Methods
Gga1 knockout mice were generated using the CRISPR/Cas9 system. Gga1-/- male mice and wild-type littermates received intraperitoneal (i.p.) injections of BPA (40 µg/kg) once daily for 2 weeks. Histological and immunofluorescence staining were performed to analyze the phenotypes of these mice.

Results
Male mice lacking Gga1 had normal fertility without any obvious defects in spermatogenesis, sperm count and sperm morphology. Gga1 ablation led to infertility in male mice exposed to BPA, along with a significant reduction in sperm count, sperm motility and the percentage of normal sperm. Histological analysis of the seminiferous epithelium showed that spermatogenesis was severely disorganized, while apoptotic germ cells were significantly increased in the Gga1 null mice exposed to BPA. Our findings suggest that Gga1 protects spermatogenesis against damage induced by environmental pollutants.
Public Response of Ipilo Village to the Flood Education Model in Gorontalo City
Authors: Achmad Fadly , Syam Husain , Asrib Ahmad Rifqi 
Published: August 2023

The purpose of this research is: 1) to provide educating to the public regarding the causes of flooding in Gorontalo City, 2) provide public understanding of the causes of flooding in their area, 3) to find out the extent of the public's response to the type of soil in their area which is one of the causes of flooding.
The research method is in the form of educating the public in the Ipilo Village which often experiences flooding in Gorontalo City. Counseling was arranged with the distribution of questionnaires. This questionnaire is to see the extent of people's understanding of the influence of soil conditions and groundwater levels as a cause of flooding and inundation.
Counseling on technical maps of soil types received a positive response from the people of Ipilo Village. This counseling provides an understanding to the public that the clay soil is dominates the Ipilo village is difficult to water infiltration and is one of the causes of flooding and inundation.
Perceptions towards Inter-Professional Education (IPE) among Pre-Registration Health Professional Students in Sri Lanka
Authors: Perera Angage Dilani Priyashanthi , Jayasinghe Halke Widanelage Udeesh Satheesha 
Published: August 2023

Introduction of Inter-Professional Education (IPE) into healthcare professions’ curricula has become an important aim globally. Health professional students experience informal interprofessional learning opportunities during their clinical training years. Investigating the attitudes toward IPE and the factors that affect these attitudes among pre-registration students in the different health professions will illuminate the needs and challenges of IPE in Sri Lanka. A cross sectional study was conducted with the students from 8 health professional groups (Medicine, Physiotherapy, Nursing Diploma/ Degree, Medical Laboratory Science (MLS), Pharmacy, Audiology, Speech and Language Therapy) at different health educational institutions. A total of 686 students were invited to complete the self-administered questionnaire. The questionnaire mainly consists of the Readiness for Inter-Professional Learning Scale (RIPLS). The RIPLS data was analyzed using factor analysis, descriptive statistics, t-test and ANOVA. The response rate for the survey was 83%. Only 18.9% of the students reported previous IPE experiences. We sought a three-factor solution but the analysis highlighted that a single factor predominates. According to the 14 items which loaded to this factor, it was named as ‘value of teamwork’. The findings suggest that students’ attitudes towards IPE are generally positive. The variable factor loading than the original validation suggests that students may need more clarity on the aim of IPE. This study suggests some focused approaches for IPE in Sri Lanka. Promoting the importance of interprofessional learning through educational methods might assist to improve the attitudes toward IPE.
Minimum Temperature Forecasting Using Gated Recurrent Unit
Authors: Subair Hilma , Selvi R. Pangayar , Vasanthi R. , Kokilavani S. , Karthick V. 
Published: August 2023

Aim: To forecast the monthly average Minimum Temperature (ºC) in Coimbatore district.
Study Design: Gated Recurrent Unit (GRU) has been employed to forecast the Minimum Temperature.
Place and Duration of Study: Time series data for average month wise Minimum Temperature from January 1982 to September 2022 was collected from Agro Climate Research Centre, TNAU for Coimbatore District.
Methodology: GRU which belongs to the field of deep learning has been employed to anticipate the average monthly Minimum Temperature by analyzing time series data from January 1982 to September 2022 in the district of Coimbatore. The model was trained using data from 1982 January to 2019 December and tested on data from 2020 January to 2022 September. After training and testing the algorithm was deployed to forecast Minimum Temperature for the lead time ahead.
Results: The GRU model generated RMSE and MAE scores of 0.694ºC and 0.523ºC, respectively, for Minimum Temperature. GRU model had a Willmott’s Index of Agreement (WI) value as 0.943 that is very close to 1. This demonstrates the effectiveness of the model built to effectively predict the Minimum Temperature. The study's evaluation of the RMSE, MAE, and Willmott Index value made it readily evident that the GRU model performed quite accurately for forecasting Minimum Temperature. Gated Recurrent Unit algorithm was used to forecast the Minimum Temperature from October 2022 till December 2023 that is for the next 15 months.
Buku Ensiklopedia Flora dan Fauna di Hutan Tropis Indonesia untuk Anak Usia 10-12 Tahun
Authors: Sanjaya Imanuel , Streit Aprilia Kartini 
Published: August 2023

Flora dan fauna pada hutan hujan tropis Indonesia sangat beragam dalam hayatinya, dan merupakan aset penting negara, bahkan merupakan aset penting dunia karena diperlukan manusia dan hewan untuk bernafas. Namun, sayangnya tidak banyak yang membahas mengenai hal ini, bahkan masyarakat banyak yang belum mengenal flora dan fauna di hutan hujan tropis. Maka dari itu diperlukan media untuk mengenalkan kembali flora dan fauna pada habitat hutan hujan tropis serta lingkungan agar anak-anak kita lebih mengenal kekayaan alam dan dapat lebih menjaga alam dan isinya. Penelitian ini menggunakan metode analisis kualitatif berdasarkan data yang terkumpul melalui studi kasus, observasi serta kuesioner yang kemudian dijabarkan secara deskriptif. Media pengenalan dilakukan melalui buku ensiklopedia. Dengan terancangnya buku ensiklopedia ini, dapat menambahkan wawasan, pengetahuan, serta lebih mengenal kekayaan alam di Indonesia agar dapat melestarikan serta memeliharanya dengan baik.
Self‐Assembly of a Tripodal Terpyridine Based Ligand with Luminescent Lanthanide Ions in Solution and Soft Material
Authors: Delente Jason M. , Kotova Oxana , Willis‐Fox Niamh , O'Brien John E. , Twamley Brendan , Daly Ronan , Gunnlaugsson Thorfinnur 
Published: January 2025

AbstractThe tripodal 1,3,5‐benzene tricarboxamide (BTA) ligand functionalized with terpyridine (1) is used to self‐assemble with Ln(III) ions (Ln = La, Sm, Eu, Gd, Tb, Yb, and Lu) in solution and within soft material (supramolecular metallogels). Stability constants for 1:1 and 3:2 (M:L) assemblies formed in MeOH are determined using nonlinear regression analysis of UV–vis absorption and emission titrations revealing a parabolic relationship between the binding constants values and the ionic radii of Ln(III) ions. The formation of gels using ligand 1 in MeOH‐H2O at 0.6 wt.% and their modification, by diffusing the Ln(III) ions through them is described. The latter leads to both crosslinking of already self‐assembled ligands, as well as modulating their physical properties as demonstrated by photophysical, rheological, and morphological studies leading to significant changes in the in situ morphology.
Subventricular Zone Microstructure in Pediatric‐Onset Multiple Sclerosis
Authors: Margoni Monica , Storelli Loredana , Pagani Elisabetta , Preziosa Paolo , Mistri Damiano , Gueye Mor , Rubin Martina , Moiola Lucia , Filippi Massimo , Rocca Maria Assunta 
Published: January 2025

ObjectiveThe aim of this study was to explore the microstructural dynamics of the subventricular zone (SVZ) with aging and their associations with clinical disability and brain structural damage in pediatric‐onset multiple sclerosis (MS) patients.MethodsOne‐hundred and forty‐one pediatric‐onset MS patients (67 pediatric and 74 adults with pediatric‐onset) and 233 healthy controls (HC) underwent neurological and 3.0 T MRI assessment. Fractional anisotropy (FA) and mean diffusivity (MD) were extracted from the SVZ and the thalamus (as control region).ResultsIn HC, SVZ FA was higher until age 40 then declined, whereas MD was lower until age 35 before rising (false discovery rate p value [pFDR] ≤ 0.008). Thalamic FA was higher until age 30 and then declined, whereas MD was higher until age 50 (pFDR ≤ 0.007). Pediatric MS patients showed significantly higher SVZ FA than pediatric HC (pFDR < 0.001), while adult patients showed no differences compared to adult HC (pFDR ≤ 0.724). Adult patients had lower thalamic FA and higher MD (pFDR < 0.001). Adults had lower SVZ FA and MD, but higher thalamic MD compared to pediatric patients (pFDR < 0.001). In pediatric MS, higher SVZ FA and MD were associated with higher white matter (WM) lesion volume (LV) and choroid plexus volume and lower brain and thalamic volumes (pFDR ≤ 0.047). In adult patients, higher SVZ MD associated with higher WM LV, lower brain volumes, and lower z‐SDMT (pFDR≤0.019). Thalamic microstructural abnormalities were associated with more severe disability and brain damage in both groups (pFDR ≤ 0.018).InterpretationOur findings suggest that microstructural changes in the SVZ occur early in pediatric MS and are associated with brain structural damage but not with clinical impairment. ANN NEUROL 2025
From Natural Product Derivative to Hexagonal Prism Supermolecule: Potent Biofilm Disintegration, Enhanced Foliar Affinity, and Effective Management of Tomato Bacterial Canker
Authors: Chen Xue , Yang Run , Liu Kongjun , Liu Min , Shi Qingchuan , Yang Jinghan , Hao Gefei , Luo Laixin , Du Fengpei , Wang Peiyi 
Published: January 2025

AbstractClavibacter michiganensis (Cmm), designated as an A2 quarantine pest by the European and Mediterranean Plant Protection Organization (EPPO), incites bacterial canker of tomato, which presently eludes rapid and effective control methodologies. Dense biofilms formed by Cmm shield internal bacteria from host immune defenses and obstruct the ingress of agrochemicals. Even when agrochemicals disintegrate biofilms, splashing and bouncing during application disperse active ingredients away from target sites. Herein, we present a supramolecular strategy to fabricate a hexagonal prism‐shaped material, BPGA@CB[8], assembled from an 18β‐glycyrrhetinic acid derivative (PBGA) and host molecule—cucurbit[8]uril (CB[8]) via host–guest recognition. This positively charged material manifests multifaceted functionalities, notably the ability to surmount biofilm barriers, annihilate the encased pathogenic bacteria, and enhance foliar affinity of droplets. The strong in vitro potency and effective deposition of BPGA@CB[8] foster optimal conditions for robust in vivo efficacy, demonstrating superior protective and curative activities (56.9 %/53.4 %) against canker of tomato at a low‐dose of 100 μg⋅mL−1 compared to BPGA (44.6 %/42.2 %), kasugamycin (30.1 %/28.4 %), and thiodiazole copper (35.4 %/31.0 %). This supramolecular material, based on natural product derivatives, provides a potent treatment for high‐risk canker of tomato, and exemplifies the utility of supramolecular strategies in optimizing the attributes of natural products for managing plant bacterial diseases.
Easily Water‐Synthesisable Iron‐Chloranilate Frameworks as High Energy and High‐Power Cathodes for Sustainable Alkali‐Ion Batteries
Authors: Durán‐Egido Víctor , Darby James P. , Cliffe Matthew J. , Garitaonandia José S. , Grande‐Fernández Paloma , Morris Andrew J. , Carretero‐González Javier , Castillo‐Martínez Elizabeth 
Published: January 2025

AbstractAchieving high battery performance from low‐cost, easily synthesisable electrode materials is crucial for advancing energy storage technologies. Metal–organic frameworks (MOFs) combining inexpensive transition metals and organic ligands are promising candidates for high‐capacity cathodes. Iron‐chloranilate‐water frameworks are herein reported to be produced in aqueous media under mild conditions. Removal of reticular water from known [Fe2(CAN)3(H2O)4] ⋅ 4H2O yields a new supramolecular metal–organic framework (SMOF), [Fe2(CAN)3(H2O)4]. Removing coordination water, a new 2D honeycomb‐like MOF forms, Fe2(CAN)3, stable without counterions and solvent. This MOF adopts the unusual ABC layer‐stacking, as determined using a combination of ab initio random structure searching, electron diffraction, and Rietveld refinement of powder XRD data. Magnetometry, Mossbauer and Raman spectroscopy confirm that all three [Fe2(CAN)3(H2O)x]⋅yH2O phases contain HS‐Fe3+ and CAN2−, with magnetic ordering temperatures increasing (5→20 K) as the Fe−CAN connectivity increases. The SMOF and MOF show reversible (de)insertion of >4Li+/f.u. at average 2,59 V and 2,76 V vs Li+/Li, respectively. [Fe2(CAN)3] achieves 146 mAh/g at 1 C, thus specific energy (563 Wh/kg) and power (446 W/kg) in Li half‐cells competitive with conventional LiFePO4 (~580 Wh/kg and ~450 W/kg). Beyond Li, [Fe2(CAN)3] delivers 394 Wh/kg and 421 Wh/kg, for Na and K half‐cells respectively, becoming a competitive cathode for sustainable batteries.
High‐Efficiency Carbon Perovskite Solar Cells via Cathode Interface Engineering by using CuPc Hole‐Transporting Layers
Authors: Zaman Zohreh , Shahroosvand Hashem , Bellani Sebastiano , Bonaccorso Francesco , Nazeeruddin Mohammad Khaja 
Published: January 2025

AbstractCarbon perovskite solar cells (C‐PSCs) represent a promising photovoltaic (PV) technology that addresses the long‐term operating stability needed to compete with commercial Si solar cells. However, the poor interface contacts between the carbon electrode and the perovskite result in a gap between C‐PSC′s performances and state‐of‐the‐art PSCs based on metallic back electrodes. In this work, Cu (II) phthalocyanine (CuPc) was rediscovered as an effective hole‐transporting material (HTM) to be coupled with carbon electrodes. In particular, based on computational studies and VASP calculations, it is found that the tetragonal structure of CuPc could efficiently coordinated to perovskite (P) layer via N and Cu atoms to Pb and I atoms, respectively. By systematically optimizing the concentration of the CuPc HTL solution, and screening the coupling of CuPc HTL with two types of carbon electrodes, based on carbon black:graphite (C‐G) mixture and reduced graphene oxide (RGO), respectively, a maximum power conversion efficiency (PCE) of 21.4 % (the mean PCE value of 18.57 %) has been achieved. In addition, our cells exhibit satisfactory stability under thermal aging at 85°C, showing less than 20% PCE loss after more than 200 hours. Furthermore, they maintain excellent shelf‐life stability, with only 1.3% PCE loss over 20 days under ambient conditions (ISOS‐D1). These findings represent a significant step forward in developing commercially competitive C‐PSCs, as they combine both high PCE and stability.
Continuous Production of Influenza VLPs Using IC‐BEVS and Multi‐Stage Bioreactors
Authors: Correia Ricardo , Zotler Taja , Ferraz Francisco , Fernandes Bárbara , Graça Miguel , Pijlman Gorben P. , Alves Paula M. , Roldão António 
Published: January 2025

ABSTRACTThe insect cell‐baculovirus expression vector system (IC‐BEVS) has been an asset to produce biologics for over 30 years. With the current trend in biotechnology shifting toward process intensification and integration, developing intensified processes such as continuous production is crucial to hold this platform as a suitable alternative to others. However, the implementation of continuous production has been hindered by the lytic nature of this expression system and the process‐detrimental virus passage effect. In this study, we implemented a multi‐stage bioreactor setup for continuous production of influenza hemagglutinin‐displaying virus‐like particles (HA‐VLPs) using IC‐BEVS. A setup consisting of one Cell Growth Bioreactor simultaneously feeding non‐infected insect cells to three parallel Production Bioreactors operated at different residence times (RT) (18, 36, and 54 h) was implemented; Production Bioreactors were continuously harvested. Two insect cell lines (neutral pH–adapted High Five and Sf9) and two recombinant baculovirus (rBAC) constructs (one that originates from a bacmid, rBACbacmid, and another of non‐bacteria origin, rBACflashbac) were tested. Combining rBACflashbac with Sf9 cells was the most efficient approach, allowing consistent HA‐VLPs titers (34 ± 14 HA titer/mL) and rBAC titers (108–109 pfu/mL) throughout the period of continuous operation (20 days). Cell growth kinetics and viability varied across RT, and higher RT was associated with increased expression of HA‐VLPs, independent of the cell line and rBAC used; RT of 54 h allowed to maximize titers. The presence of particles resembling HA‐VLPs was confirmed by transmission electron microscopy throughout the continuous operation. This work showcases the implementation of a process for continuous production of a promising class of biotherapeutics (i.e., VLPs), and paves the way for establishing continuous, integrated setups using the IC‐BEVS expression system.
A Novel Screening System to Characterize and Engineer Quorum Quenching Lactonases
Authors: Sompiyachoke Kitty , Bravo Joseph , Sikdar Rakesh , Abdullah Jowan , Elias Mikael H. 
Published: January 2025

ABSTRACTN‐acyl l‐homoserine lactones are signaling molecules used by numerous bacteria in quorum sensing. Some bacteria encode lactonases, which can inactivate these signals. Lactonases were reported to inhibit quorum sensing‐dependent phenotypes, including virulence and biofilm. As bacterial signaling is dependent on the type of molecule used, lactonases with high substrate specificity are desirable for selectively targeting species in communities. Lactonases characterized from nature show limited diversity in substrate preference, making their engineering appealing but complicated by the lack of convenient assays for evaluating lactonase activity. We present a medium‐throughput lactonase screening system compatible with lysates that couples the ring opening of N‐acyl l‐homocysteine thiolactones with 5,5‐dithio‐bis‐(2‐nitrobenzoic acid) to generate a chromogenic signal. We show that this system is applicable to lactonases from diverse protein families and demonstrate its utility by screening mutant libraries of GcL lactonase from Parageobacillus caldoxylosilyticus. Kinetic characterization corroborated the screening results with thiolactonase and homoserine lactonase activity levels. This system identified GcL variants with altered specificity: up to 1900‐fold lower activity for long‐chain N‐acyl l‐homoserine lactone substrates and ~38‐fold increase in preference for short‐chain substrates. Overall, this new system substantially improves the evaluation of lactonase activity and will facilitate the identification and engineering of quorum quenching enzymes.