bluuebunny/crossref_metadata_2025_split huggingface dataset:
The stereoselective bioactivity and mechanism of indoxacarb against Spodoptera frugiperda
Authors: Guo Haoming , Xiao Nan , Wang Zhixuan , Liang Yabo , Liu Xueke , Wang Peng , Liu Donghui 
Published: January 2025

AbstractBACKGROUNDIndoxacarb, a type of chiral pesticide, is used to control Lepidoptera insects. Most studies had focused on the environmental behavior and selective toxicity of indoxacarb enantiomers, and the mechanism behind its selective biological activity against target organisms is not well understood.RESULTSSpodoptera frugiperda was selected as the target insect. The lethal concentrations (96 h) of indoxacarb enantiomers on S. frugiperda were 2.61 mg/kg (S‐indoxacarb) and 463.52 mg/kg (R‐indoxacarb). S‐Indoxacarb resulted in stronger oxidative damage to S. frugiperda than R‐indoxacarb, and catalase and malondialdehyde were upregulated by 40.46% and 68.64% respectively after treatment with S‐indoxacarb. Furthermore, cytochrome P450 and carboxylesterase were activated by S‐indoxacarb, increasing by 39.62% and 63.68% respectively. Decarbomethoxyllated JW062 (DCJW), a metabolite of indoxacarb, has insecticidal activity. The concentration of DCJW in the S‐indoxacarb treatment group was 2.73 times that in the R‐indoxacarb treatment group. Molecular docking results demonstrated that S‐indoxacarb could spontaneously bind to metabolic enzymes and be metabolized.CONCLUSIONSEnantiomeric bioactivity of indoxacarb enantiomers against on S. frugiperda was observed. S‐Indoxacarb demonstrated remarkable insecticidal efficacy. Upon ingestion by S. frugiperda, it induced oxidative damage. Furthermore, S‐indoxacarb was metabolized to DCJW, which has a significant role in its insecticidal properties. The selective bioactivity of indoxacarb enantiomers in S. frugiperda might be attributed to the enantiomeric metabolites. These findings offer a new perspective on the selective mechanisms of chiral pesticides. © 2025 Society of Chemical Industry.
Optimizing Laccase Production From Halotolerant Enterobacter sp. GR18 for Hydrocarbon Bioremediation
Authors: Hosseini Seyyed Mohammad , Sepahi Abbas Akhavan , Razavi Mohamad Reza , Saffarian Parvaneh 
Published: January 2025

ABSTRACTHydrocarbon contamination, primarily from oil spills and their derivatives, poses significant environmental challenges and has profound impacts on public health and ecosystems. Innovative bioremediation strategies, especially those utilizing laccase enzymes, have emerged as robust and sustainable solutions to degrade these persistent pollutants. This study focused on optimizing laccase production from Enterobacter sp. GR18, isolated from the Grawan mineral spring near Sardasht, West Azerbaijan Province, Iran. The bacterium was identified through comprehensive biochemical tests and 16S rRNA sequencing. Advanced methodologies were used to optimize laccase production conditions. The purified laccase enzyme was characterized using SDS‐PAGE. The effects of various carbon and nitrogen sources, metal ions, and inducers on enzymatic activity were thoroughly investigated. Structural changes in crude oil samples treated with laccase were analyzed using nuclear magnetic resonance (NMR) spectroscopy. Enterobacter sp. GR18 showed exceptional laccase activity among 33 isolated strains. Glucose and yeast extract were the most effective carbon and nitrogen sources, respectively. Copper and iron ions significantly enhanced enzyme activity. NMR spectroscopy indicated substantial breakdown of complex hydrocarbons in crude oil samples posttreatment, demonstrating the enzyme's effectiveness in hydrocarbon degradation. This study reveals the potential of Enterobacter sp. GR18 for high laccase production and efficient hydrocarbon degradation under extreme environmental conditions. These findings can catalyze the development of robust and sustainable bioremediation technologies to address hydrocarbon contamination.
Pristine and Alkaline Earth Metal‐Decorated C24N24 Fullerenes as Potential Sensors for Halomethanes: A Comparative DFT Investigation
Authors: Ibrahim Mahmoud A. A. , Moussa Nayra A. M. , Rady Al‐shimaa S. M. , Mekhemer Gamal A. H. , El‐Tayeb Mohamed A. , Khan Shahzeb , Soliman Mahmoud E. S. 
Published: January 2025

AbstractIn this paper, attempts were made to investigate the adsorption potential of pristine and alkaline earth metal (AEM)‐decorated C24N24 fullerenes toward the halomethanes (XCH3, where X═F, Cl, and Br). By means of DFT calculations, the XCH3⋯C24N24 and ⋯AEM@C24N24 complexes (AEM═Be and Mg) were adequately examined. Upon energetic features, the FCH3⋯Mg@C24N24 complex exhibited the most negative adsorption and interaction energies with values of −21.01 and −22.61 kcal/mol, respectively. From thermodynamic analysis, spontaneous and exothermic natures of XCH3⋯Mg@C24N24 interactions were affirmed, unveiling favorable role of Mg decoration in enhancing the adsorption process. From SAPT analysis, the electrostatic forces dominated the interactions within the XCH3⋯C24N24 and ⋯Be/Mg@C24N24 complexes. Upon FMOs analysis, notable alterations in the distribution of molecular orbitals of studied fullerenes were noticed, indicating the effect of XCH3 adsorption on the electronic features of the studied fullerenes. Further, the Egap values were decreased after the adsorption process which enhanced electrical conductivity of studied fullerens. From DOS plots, the capacity of the C24N24 and Be/Mg@C24N24 to adsorb the XCH3 molecules was affirmed. Solvation energies demonstrated the favorability of the studied adsorption process in the water phase. The present findings established C24N24 and AEM@C24N24 fullerenes as potential effective candidates for detecting halomethanes.
Cost‐Effective RuNi Solid Solutions Prepared by Electrodeposition for Efficient Alkaline Hydrogen Evolution
Authors: Weng Yibo , Li Qingqing , Li Kaikai 
Published: January 2025

AbstractThe development of efficient hydrogen evolution reaction (HER) catalysts is crucial for water electrolysis. Currently, Ru‐based catalysts are considered top contenders, but issues with stability, activity, and cost remain. In this work, RuNi alloys possessing a solid solution structure within the Ru lattice are prepared via straightforward electrodeposition on various substrates and assessed as HER catalysts in alkaline media. A RuNi solid solution containing 9.8 at. % Ni deposited on Ti substrate, wherein the Ni content greatly surpasses the solubility limit of Ni in Ru at room temperature, exhibits a considerably low overpotential of 28 mV at a current density of 10 mA cm−2, along with good long‐term stability (less than 100 mV increase in overpotential after 600 h). The enhancement in HER performance results from the increased electron density around Ru atoms due to Ni coordination, which facilitates the desorption of H* from the catalyst surface to produce H2. Concurrently, incorporating Ni reduces the Ru usage, rendering the RuNi alloy a viable cost‐effective HER catalyst for practical applications.
A Highly Ductile Composite of 3D‐Printed Poly(Lactic Acid) With InSe Particles and Flakes as a Filler
Authors: Li Huihui , Yu Zhongliang , Liu Bowen , Gao Yang , Liu Ming , Zhang Jianqi , Ruoff Rodney S. , Wang Bin 
Published: January 2025

ABSTRACTThe biodegradable polymer poly(lactic acid) (PLA) is brittle. PLA‐based composites reinforced by indium selenide (InSe) particles or flakes are prepared; each is found to have outstanding plasticity. InSe nanosheets are prepared by sonication of solid InSe in N‐methyl pyrrolidone, followed by washing/dispersion in ethanol, and subsequent drying. These InSe nanosheets, or in separate studies InSe particles, are mixed with PLA to make composite materials. The PLA composite materials are 3D‐printed into “dogbone” samples that are tensile‐loaded. The optimum dogbone specimen is 1.5 times stronger and 5.5 times tougher than neat PLA specimens prepared in the same way. To the best of our knowledge, this concurrent improvement in tensile strength and toughness has not been achieved before in PLA with any filler type. Finite element analysis, together with experimental analysis of (i) fracture surfaces, (ii) the PLA crystal structure, and (iii) the internal structure by micro‐CT scanning, suggests that the exceptional mechanical performance is due to the intrinsic properties of InSe and, particularly, the emergence of crack shielding and crack deflection at the interfaces of PLA and InSe flakes. These findings indicate that PLA–InSe composites may offer opportunities to broaden the applications of PLA composites, including as load‐bearing materials.
Redefining intelligence: collaborative tinkering of healthcare professionals and algorithms as hybrid entity in public healthcare decision-making
Authors: van Voorst Roanne 
Published: January 2025

AbstractThis paper analyzes the collaboration between healthcare professionals and algorithms in making decisions within the realm of public healthcare. By extending the concept of ‘tinkering’ from previous research conducted by philosopher Mol (Care in practice. On tinkering in clinics, homes and farms Verlag, Amsterdam, 2010) and anthropologist Pols (Health Care Anal 18: 374–388, 2009), who highlighted the improvisational and adaptive practices of healthcare professionals, this paper reveals that in the context of digitalizing healthcare, both professionals and algorithms engage in what I call ‘collaborative tinkering’ as they navigate the intricate and unpredictable nature of healthcare situations together. The paper draws upon an idea that is increasingly common in academic literature, namely that healthcare professionals and the algorithms they use can form a hybrid decision-making entity, challenging the conventional notion of agency and intelligence as being exclusively confined to individual humans or machines. Drawing upon an international, ethnographic study conducted in different hospitals around the world, the paper describes empirically how humans and algorithms come to decisions together, making explicit how, in the practice of daily work, agency and intelligence are distributed among a range of actors, including humans, technologies, knowledge resources, and the spaces where they interact. The concept of collaborative tinkering helps to make explicit how both healthcare professionals and algorithms engage in adaptive improvisation. This exploration not only enriches the understanding of collaborative dynamics between humans and AI but also problematizes the individualistic conception of AI that still exists in regulatory frameworks. By introducing empirical specificity through ethnographic insights and employing an anthropological perspective, the paper calls for a critical reassessment of current ethical and policy frameworks governing human–AI collaboration in healthcare, thereby illuminating direct implications for the future of AI ethics in medical practice.
In vitro culture and confocal microscopy study of Maritrema gratiosum Nicoll, 1907 (Digenea): From metacercaria to ovigerous adult
Authors: Chuang YuChing , Shinn Andrew P. , Bron James E. 
Published: January 2025

Abstract
This study set out to characterise the in vitro development, including musculature, of the microphallid parasite of the barnacle Semibalanus balanoides (Linnaeus, 1767), Maritrema gratiosum Nicoll, 1907 collected in Scotland. An in vitro culture model was developed to obtain ovigerous adults of M. gratiosum and their morphology was observed. Different media were tested and NCTC-109 was chosen as the best medium. The effects of different concentrations of serum upon adult longevity, size and egg production was measured. Survival for 10-days was achieved when flukes were cultured in NCTC-109 plus chicken serum and antibiotics. Forty percent chicken serum seemed to provide better results in terms of survival time and producing flukes with the largest body lengths. Both normal and abnormal eggs were observed from adults cultured in vitro. Confocal microscopy was undertaken to provide details of the development of the parasite’s ultrastructure, including musculature, during the course of in vitro culture. While the musculature of M. gratiosum was similar to that of other microphallids, some additional novel structures were observed, most notably a ligament connecting pars prostatica and seminal vesicle and a racket-shaped excretory bladder. This study has provided greater insight into the biology M. gratiosum, and also developed a good in vitro model which might be applied to ecological or medical research in the future.
Human organoids for rapid validation of gene variants linked to cochlear malformations
Authors: Zafeer Mohammad Faraz , Ramzan Memoona , Duman Duygu , Mutlu Ahmet , Seyhan Serhat , Kalcioglu M. Tayyar , Fitoz Suat , DeRosa Brooke A. , Guo Shengru , Dykxhoorn Derek M. , Tekin Mustafa 
Published: January 2025

AbstractDevelopmental anomalies of the hearing organ, the cochlea, are diagnosed in approximately one-fourth of individuals with congenital. The majority of patients with cochlear malformations remain etiologically undiagnosed due to insufficient knowledge about underlying genes or the inability to make conclusive interpretations of identified genetic variants. We used exome sequencing for the genetic evaluation of hearing loss associated with cochlear malformations in three probands from unrelated families deafness. We subsequently generated monoclonal induced pluripotent stem cell (iPSC) lines, bearing patient-specific knockins and knockouts using CRISPR/Cas9 to assess pathogenicity of candidate variants. We detected FGF3 (p.Arg165Gly) and GREB1L (p.Cys186Arg), variants of uncertain significance in two recognized genes for deafness, and PBXIP1(p.Trp574*) in a candidate gene. Upon differentiation of iPSCs towards inner ear organoids, we observed developmental aberrations in knockout lines compared to their isogenic controls. Patient-specific single nucleotide variants (SNVs) showed similar abnormalities as the knockout lines, functionally supporting their causality in the observed phenotype. Therefore, we present human inner ear organoids as a potential tool to validate the pathogenicity of DNA variants associated with cochlear malformations.
Graphical Abstract
Intra-individual comparison of long-term outcomes between combined and indirect bypass surgery in adult moyamoya disease
Authors: Chung Yuwhan , Kim Jeong Eun , Kang Hyun-Seung , Kim Tae Young , Paeng Jin Chul , Cho Won-Sang , Lee Sung Ho , Ha Eun Jin , Kim Kangmin 
Published: January 2025

Abstract
Purpose
Bypass surgery is regarded as the standard treatment option for symptomatic and hemodynamically unstable moyamoya disease (MMD). However, there is ongoing debate about the most effective type of bypass surgery. We aimed to analyze the long-term outcomes of combined and indirect bypasses for MMD patients through intra-individual comparisons.

Methods
Of the 896 patients who underwent 1084 bypass surgeries between 2007 and 2021, 24 patients with MMD who underwent combined bypass on one side and indirect bypass on the other side were ultimately enrolled in this study. Clinical, angiographic and hemodynamic outcomes were retrospectively evaluated.

Results
Three asymptomatic strokes (12.5%) occurred within 30 postoperative days in each group. Postoperative strokes after 30 days occurred in 3 patients (12.5%) with 3 hemorrhagic events and 1 cerebral infarction, only in indirect bypass, while no stroke occurred in hemispheres treated with combined bypass. The revascularization area relative to supratentorial area was significantly greater in combined bypass than in indirect bypass, both in short-term and long-term periods (64.9% versus 43.9% in short-term and 75.7% versus 54.9% in long-term; P < .001, respectively). Hemodynamic outcomes showed significantly greater increases in acetazolamide-challenged cerebral blood flow (CBFacz) during short-term follow-up (P = .04) and in both basal CBF (CBFbas) and CBFacz during long-term follow-up (P = .014 and P = .009, respectively) in combined bypass than in indirect bypass.

Conclusion
Combined bypass may be a more effective treatment option for MMD based on its higher revascularization area and favorable hemodynamic results compared to indirect bypass in the same patient.
Mild behavioral impairment in idiopathic REM sleep behavior disorder and Lewy body disease continuum
Authors: Jin Bora , Yoon Eun Jin , Woo Kyung Ah , Kim Seoyeon , Lee Seungmin , Kim Ryul , Shin Jung Hwan , Kim Yu Kyeong , Lee Jee-Young 
Published: January 2025

AbstractTo investigate the clinical impact of mild behavioral impairment (MBI) in a predefined cohort with Lewy body disease (LBD) continuum. Eighty-four patients in the LBD continuum participated in this study, including 35 patients with video-polysomnography-confirmed idiopathic REM sleep behavior disorder (iRBD) and 49 clinically established LBD. Evaluations included the Movement Disorder Society-Unified Parkinson’s Disease Rating Scale (MDS-UPDRS), neuropsychological tests, and MBI Checklist (MBI-C). We examined the clinical associates of MBI-C and its diagnostic values in predicting disease severity and cognitive impairment across the LBD continuum. Participants were classified into 19 cognitively normal (CN), 45 mild cognitive impairment (MCI), and 20 dementia groups. Median MBI-C total scores were 1.0, 8.0, and 18.5 for CN, MCI, and dementia groups, respectively, with a significant increasing trend (p < 0.001). The MBI-C total score demonstrated significant correlations with the MDS-UPDRS part 1 (r = 0.504, p < 0.001) and total scores (r = 0.508, p < 0.001). Furthermore, significant correlations were observed between MBI-C and cognitive performances in frontal/executive (DSC: r = −0.314, p = 0.006; TMT-B: r = −0.338, p = 0.003) and attentional (TMT-A: r = −0.343, p = 0.002) domains. A cutoff 5.0 effectively differentiated the MCI from CN groups (area under the curve (AUC = 0.74). Furthermore, the MBI-C effectively discriminated the iRBD patients with high phenoconversion risk against those with low-risk (cut-off 4.0, AUC = 0.72). The MBI-C may be a useful screening questionnaire reflecting clinical severity across the LBD continuum. Longitudinal studies are needed to determine its value in monitoring disease progression in prodromal LBD.
Two experimental methods to integrate intra-oral scans into 3D stereophotogrammetric facial images
Authors: Schobben Reinout R.P. , Rangel Frits A. , Bruggink Robin , Crins - de Koning Marjolein L.D. , Bronkhorst Ewald M. , Ongkosuwito Edwin M. 
Published: January 2025

Abstract

Objectives
For this research two different ways for integrating intra-oral scans into three-dimensional (3D) stereophotogrammetric images are analyzed and compared to the gold standard method.

Materials and methods
A cross-sectional study was performed. For each patient a complete dataset was collected, which was used to generate 3D fusion models by three different methods: method A using cheek retractors, method B using a tracer and method C using full-skull CBCT. The experimental methods A and B were compared to the gold standard method C.

Results
A group of eighteen patients were included in this study. The translation (X, Y,Z), the euclidean distance and the rotation (roll, pitch, yaw) were calculated for both experimental methods A and B in comparison with the gold standard method C. Twelve out of fourteen measurements were clinically acceptable (below 2 mm or 2 degrees). Method A shows the highest deviation in the pitch-orientation for rotation (2.51 degrees, 95% CI [1.756 … 3.272]), while method B shows a higher deviation along the y-axis (1.85 mm, 95% CI [1.224 … 2.467]).

Conclusions
This study shows promising results of non-ionizing methods to integrate intra-oral scans into 3D stereophotogrammetric images. With improved accuracy in pitch in method A and translation along the Y-axis in method B, all measurements will be within the clinically acceptable threshold. However, since these two measurements exceed the clinically acceptable thresholds, the complete model positioning is less accurate. Therefore the main goal in further research should be to improve the accuracy of the pitch in method A and the translation along the Y-axis in method B. Additionally, for clinical use the biggest improvement could be gained by optimizing the clinical workflow and data processing.

Clinical relevance
By using a non-ionizing 3D fusion model instead of a conventional cephalogram for treatment planning, the ionizing dose during orthodontic treatment can be significantly reduced.
Reliability and validity of the Londrina activities of daily living protocol in individuals with systemic sclerosis
Authors: Gedikli Yasemin , Ozcan Kahraman Buse , Birlik Merih , Acar Serap , Savci Sema 
Published: January 2025

Abstract

Purpose
To investigate the validity and reliability of the Londrina ADL Protocol in patients with systemic sclerosis (SSc).

Methods
The study included 39 individuals with SSc and 30 healthy participants aged 18-70 years. Performance-related ADL assessment was performed with the Londrina ADL Protocol which was performed twice by the same rater and energy expenditure during the test with the Dynaport Move Monitor device. Functional capacity (6 Minute Walk Test-6MWT), upper extremity muscle strength (hand-held dynamometer), hand and finger grip strength (hand dynamometer and pinch meter), upper extremity functionality (Q-Dash Questionnaire and 9 Hole Peg Test), self-reported ADL (Milliken ADL Scale (MAS)) and quality of life (SF-36 questionnaire) were evaluated.

Results
Upper extremity muscle strength, functional capacity, upper extremity functionality, and quality of life of individuals with SSc were found to be significantly lower and the time spent by individuals with SSc in the Londrina ADL Protocol was lower and the time spent by individuals with SSc in the Londrina ADL Protocol was significantly longer compared to the healthy group (p<0.05). Performance in the the Londrina ADL had high intraclass correlation coefficient ICC=0.813. A moderate negative correlation was found between the time to complete the Londrina Protocol and 6MWT (p<0.05), and a weak negative correlation was found between dominant side hand grip strength (p<0.05) and MAS (p<0.05) in individuals with SSc. A moderate positive correlation was found between the Londrina Protocol and the 9-Hole Peg Test for the dominant side (p<0.05).

Conclusion
The Londrina Protocol is a valid and reliable method, hence, this makes it appropriate for an objective, performance-based evaluation of ADL in SSc population.

Key Points• The results obtained by reapplication the Londrina ADL Protocol under the same conditions showed that the Londrina ADL Protocol is a highly reliable assessment method in scleroderma patients.• Considering the activities and protocol content of the Londrina ADL Protocol, it is thought to show functional performance better than the Glittre ADL test and 6MWT.• The Londrina ADL Protocol is appropriate for objective and performance-based assessment of ADL in the SSc population for both research and clinical practice.