bluuebunny/crossref_metadata_2025_split huggingface dataset:
891 Next Generation Sequencing Mutational Profiles of Gliomas Presenting With and Without Seizure
Authors: Shukla Poojan , Nguyen Minh P. , Young Jacob , Lee Anthony T. , Chang Edward 
Published: March 2023

INTRODUCTION:
Seizures are among the most common presentation of gliomas in adults, but much remains unknown regarding the relationship between underlying tumor biology and epileptic activity.

METHODS:
We conducted a single-center retrospective review of 140 adult patients who underwent surgical management of glioma with next generation sequencing of tumor samples obtained 2016-2020. Univariate odds ratios were computed for relevant cohort characteristics.

RESULTS:
Among patients with documented seizure history, 71 (51.1%) presented with seizures while 68 (48.9%) did not. At time of diagnosis, patients presenting with seizure were younger (47 vs 60.5, p = 0.0001) and had more recurrent disease (60.6% vs 16.7%, p < 0.0001). Patients presenting with seizure had fewer glioblastomas (OR: 0.2 [0.09-0.41], p < 0.0001) but more astrocytomas (OR: 4.78 [1.68-13.65], p = 0.0038) and oligodendrogliomas (OR: 3.47 [1.07-11.27], p = 0.0557). Of the top 15 most common mutations in our cohort, patients presenting with seizure had fewer instances of IDH1 (OR: 5.26 [2.44-11.32], p<0.0001), polysomy 7/monosomy 10 (OR: 0.48 [0.24-0.95], p = 0.0523), and PTEN mutation (OR: 0.46 [0.22-0.98], p = 0.0646), but more instances of 1p/19q codeletion (OR: 3.47[1.07-11.27], p = 0.0557), ATRX (OR: 2.54 [1.15-5.59], p = 0.0315) and CIC (OR: 13.63 [1.72-107.97], p = 0.0046) mutations. Notably, mutations in NF1 (OR: 0.73 [0.28-1.88)], p = 0.6753), TP53 (OR: 1.1 [0.56-2.15], p = 0.9194), TERT (OR: 0.63 [0.31-1.28], p = 0.2679), CDKN2A/B (OR: 0.61 [0.31-1.21], p = 0.2154), and EGFR (OR: 0.49 [0.21-1.13], p = 0.1396) were not associated with seizure presentation.

CONCLUSIONS:
ATRX and CIC mutations are positively associated with seizure presentation while polysomy 7/monosomy 10 and PTEN mutations are negatively associated. Future analysis of this cohort is needed to better characterize the influence of tumor mutational profile on preoperative as well as postoperative seizure activity.
The role of O-GlcNAcylation in development
Authors: Czajewski Ignacy , van Aalten Daan M. F. 
Published: March 2023

ABSTRACT
O-GlcNAcylation is a dynamic post-translational modification performed by two opposing enzymes: O-GlcNAc transferase and O-GlcNAcase. O-GlcNAcylation is generally believed to act as a metabolic integrator in numerous signalling pathways. The stoichiometry of this modification is tightly controlled throughout all stages of development, with both hypo/hyper O-GlcNAcylation resulting in broad defects. In this Primer, we discuss the role of O-GlcNAcylation in developmental processes from stem cell maintenance and differentiation to cell and tissue morphogenesis.
Microbiota Mediate Enhanced Exercise Capacity Induced by Exercise Training
Authors: DOWDEN ROBERT A. , WISNIEWSKI PAUL J. , LONGORIA CANDACE R. , OYDANICH MARKO , MCNULTY TARA , RODRIGUEZ ESTHER , ZHANG JIE , CAVALLO MARK , GUERS JOHN J. , VATNER DOROTHY E. , VATNER STEPHEN F. , CAMPBELL SARA C. 
Published: March 2023

ABSTRACT
The gut microbiota is critical to host metabolism and is influenced by many factors, including host genotype, diet, and exercise training.

Purpose
We investigated the effects of gut microbes, and the mechanisms mediating the enhanced exercise performance induced by exercise training, i.e., skeletal muscle blood flow, and mitochondrial biogenesis and oxidative function in male mice.

Methods
All mice received a graded exercise test before (PRE) and after exercise training via forced treadmill running at 60% to 70% of maximal running capacity 5 d·wk−1 for 5 wk (POST). To examine the role of the gut microbes, the graded exercise was repeated after 7 d of access to antibiotic (ABX)-treated water, used to eliminate gut microbes. Peripheral blood flow, mitochondrial oxidative capacity, and markers of mitochondrial biogenesis were collected at each time point.

Results
Exercise training led to increases of 60% ± 13% in maximal running distance and 63% ± 11% work to exhaustion (P < 0.001). These increases were abolished after ABX (P < 0.001). Exercise training increased hindlimb blood flow and markers of mitochondrial biogenesis and oxidative function, including AMP-activated protein kinase, sirtuin-1, PGC-1α citrate synthase, complex IV, and nitric oxide, all of which were also abolished by ABX treatment.

Conclusions
Our results support the concept that gut microbiota mediate enhanced exercise capacity after exercise training and the mechanisms responsible, i.e., hindlimb blood flow, mitochondrial biogenesis, and metabolic profile. Finally, results of this study emphasize the need to fully examine the impact of prescribing ABX to athletes during their training regimens and how this may affect their performance.
Can We Accurately Predict Critical Power and W′ from a Single Ramp Incremental Exercise Test?
Authors: CAEN KEVIN , BOURGOIS JAN G. , STUER LENA , MERMANS VINCENT , BOONE JAN 
Published: March 2023

ABSTRACT

Purpose
The purpose of this study was to examine the suitability of a single ramp incremental test to predict critical power (CP) and W′. We hypothesized that CP would correspond to the corrected power output (PO) at the respiratory compensation point (RCP) and W′ would be calculable from the work done above RCP.

Methods
One hundred fifty-three healthy young people (26 ± 4 yr, 51.4 ± 7.6 mL·min−1·kg−1) performed a maximal ramp test (20, 25, or 30 W·min−1), followed by three to five constant load trials to determine CP and W′. CP and W′ were estimated using a “best individual fit” approach, selecting the mathematical model with the smallest total error. The RCP was identified by means of gas exchange analysis and then translated into its appropriate PO by applying a correction strategy in order to account for the gap in the V̇O2/PO relationship between ramp and constant load exercise. We evaluated the agreement between CP and the PO at RCP, and between W′ and the total work done above CP (W′
RAMP > CP) and above RCP (W′
RAMP > RCP) during the ramp test.

Results
The CP was significantly higher than the PO at RCP (Δ = 8 ± 16 W, P < 0.001). W′
RAMP > CP was significantly lower than W′ (Δ = 1.9 ± 3.3 kJ, P < 0.001), whereas W′
RAMP > RCP and W′ did not differ from each other (Δ = −0.6 ± 5.8 kJ, P = 0.21).

Conclusions
Despite the fact that CP and RCP occurred in close proximity, the estimation of W′ from ramp exercise may be problematic given the likelihood of underestimation and considering the large variability. Therefore, we do not recommend the interchangeable use of CP and W′ values derived from constant load versus ramp exercise, in particular, when the goal is to obtain accurate estimates or to predict performance capacity.
Effect of Cold Exposure and Exercise on Insulin Sensitivity and Serum Free Fatty Acids in Obese Rats
Authors: WENG XIQUAN , WANG CHAOGE , YUAN YU , WANG ZHENHUAN , KUANG JUJIAO , YAN XU , CHEN HAO 
Published: March 2023

ABSTRACT

Purpose
This study aimed to explore the effect of exercise and cold exposure on insulin sensitivity and the level of serum free fatty acids (FFA) in diet-induced obese rats.

Methods
Sixty-four diet-induced obese rats were randomly assigned to eight groups: room temperature–sedentary, room temperature–exercise, acute cold exposure–sedentary, acute cold exposure–exercise, intermittent cold exposure–sedentary, intermittent cold exposure–exercise, sustained cold exposure–sedentary, and sustained cold exposure–exercise. After the interventions, the homeostatic model assessment for insulin resistance (HOMA-IR) values, the level of serum FFA, subcutaneous fat ratio (SFR) and visceral fat ratio, enzyme activities of adipose triglyceride lipase, and lipoprotein lipase (LPL) in inguinal adipose tissue, and protein expression of PGC1-α and p38 MAPK in skeletal muscle were investigated.

Results
We found that exercise (P = 0.0136) and cold exposure (P < 0.0001) reduced HOMA-IR values independently. Exercise reduced serum FFA (P = 0.0041), whereas cold exposure did not affect them. Moreover, the HOMA-IR values were positively correlated with the serum FFA levels (r = 0.32, P = 0.01). SFR or visceral fat ratio was coordinately reduced by the interaction (for SFR, P = 0.0015) or opposing main effects between or of cold exposure and exercise, supporting the reduction of serum FFA. However, cold exposure or exercise increased the activity of adipose triglyceride lipase and LPL independently or interactively (for LPL, P = 0.0143), suggesting an increase in serum FFA. Finally, cold exposure and exercise enhanced protein expression of PGC1-α and p38 MAPK independently or interactively (for p38 MAPK, P = 0.0226), suggesting increased uptake and oxidation of serum FFA in muscle.

Conclusions
These results suggest that the combination of exercise and cold exposure may result in more serum FFA utilization than production and thus lead to reduced serum FFA and increased insulin sensitivity.
Sargassum sp. extract improve hematological profile of tilapia fish (Oreochromis niloticus)
Authors: Gazali Mohamad , Effendi Irwan , Husni Amir , Nurjanah Nurjanah , Wahyuni Sri , Kurniawan Ronal 
Published: March 2023

Background: Strategies to increase body resistance and prevent disease in aquaculture include using vaccines, antibiotics, and probiotics. Today, the use of antibiotics with natural ingredients is becoming a trend. One of the natural ingredients that contain high antioxidants and antibiotics is Sargassum sp. Methods: This research was conducted from March to May 2022 at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau, in two stages: 1) the sensitivity of extracts of Sargassum sp. and 2) the application of Sargassum sp. extract orally in tilapia (O. niloticus). The parameters measured were clear zone, minimum inhibitory concentration, LD50 test of leaf extract of Sargassum sp. in tilapia (O. niloticus), hemoglobin levels, hematocrit, total leukocytes, total erythrocytes, leukocyte differentiation, and survival rate. Data on hematology parameters were tabulated and analyzed using a One-Way ANOVA followed by a Student Newman Keuls (SNK) test when deemed necessary. Results: The results showed that the extract of Sargassum sp. inhibited the growth of Aeromonas hydrophila bacteria with a clear zone of 6.5-15.0 mm, which is classified as resistant. At doses of 2000, 2500, and 3000 ppm, it did not cause death in fish for 96 hours (LD50). Hematological parameters can be a sign of the health status of fish. Tilapia given Sargassum sp. in different doses gave an effect between treatments, both after 30 days of rearing and post-test against A. hydrophila bacteria (p<0.05). The results showed that the hematology of fish fed with Sargassum sp. extract was in the normal or healthy range. Healthy tilapia had erythrocyte counts ranging from 1.34-2.11×106 cells/mm3, hematocrit 26.17-33.19%, hemoglobin 6.26-11.2 g/dL and total leukocytes 1.01-1.50×104 cells/mm3 and total erythrocytes 5.88-9.13×104 cells/ mm3. Conclusions: A dose of 3000 ppm provided the highest health improvement against A. hydrophila bacterial infection.
COMPETITIVE TEAM ENVIRONMENTS AND STUDENT LEADER CAPACITY GROWTH
Authors: Rosch David M. , Allen Scott J. , Jenkins Daniel M. , Pickett Meghan L. 
Published: March 2023

We conducted a national study of the Collegiate Leadership Competition (CLC), which since inception in 2015, has included over 75 higher education institutions. The CLC brings students together in collaborative institution-based teams to compete with other teams in competitions to achieve goals and practice effective leadership skills. Our goal was to assess leadership capacity growth over the course of a four-month team practice period through the daylong inter-team competition and evaluate participant leadership assessed several months later. Results suggested students made significant and sustainable gains in leader-self-efficacy and short-term gains in leadership skill and motivation to lead. Our results also indicated the team’s coach played a significant role in student leadership development.
Leadership development programs for students in educational settings are proliferating in number and design. Curricular programs range from academic minors and certificates to doctoral programs in a variety of academic homes (e.g., education, business, healthcare). Co-curricular programs often take the form of drop-in workshops, day-long experiences, alternative spring breaks, service-learning trips, and other programs housed in student affairs and administrative offices (Guthrie & Jenkins, 2018). Moreover, the number of programs has steadily increased over the last 15 years from just under 1,000 in 2006 (Brungardt, et al., 2006) to more than 2,000 (ILA Program Directory, 2021). And while there is some commonality among the approach of these leadership programs in terms of content and delivery (see Harvey & Jenkins, 2014), vast differences exist in the structure and learning goals of student leadership programs compared to other social science disciplines. A potentially fruitful area in which to explore its effectiveness in supporting leadership development is the environment of competitive teams, where individuals work together as a group to compete against other teams. The purpose of our research was to investigate the degree to which such a competitive environment might support or detract from student leadership group, employing a potentially effective example of a formal program that utilizes the innovative approach of team competitions to motivate learning (the CLC).
Absolute synovial polymorphonuclear neutrophil cell count as a biomarker of periprosthetic joint infection
Authors: Jandl Nico M. , Kleiss Sebastian , Mussawy Haider , Beil Frank T. , Hubert Jan , Rolvien Tim 
Published: March 2023

AimsThe aim of this study was to evaluate the diagnostic accuracy of the absolute synovial polymorphonuclear neutrophil cell (PMN) count for the diagnosis or exclusion of periprosthetic joint infection (PJI) after total hip (THA) or knee arthroplasty (TKA).MethodsIn this retrospective cohort study, 147 consecutive patients with acute or chronic complaints following THA and TKA were included. Diagnosis of PJI was established based on the 2018 International Consensus Meeting criteria. A total of 39 patients diagnosed with PJI (32 chronic and seven acute) and 108 patients with aseptic complications were surgically revised.ResultsUsing receiver operating characteristic curves and calculating the area under the curve (AUC), an optimal synovial cut-off value of 2,000 PMN/µl was determined (AUC 0.978 (95% confidence interval (CI) 0.946 to 1)). Using this cut-off, sensitivity and specificity of absolute synovial PMN count for PJI were 97.4% (95% CI 91.2 to 100) and 93.5% (95% CI 88.9 to 98.1), respectively. Positive and negative predictive value were 84.4% (95% CI 72.7 to 93.9) and 99.0% (95% CI 96.7 to 100), respectively. Exclusion of 20 patients with acute complications improved specificity to 97.9% (95% CI 94.6 to 100). Different cut-off values for THA (< 3,600 PMN/µl) and TKA (< 2,000 PMN/µl) were identified. Absolute synovial PMN count correlated strongly with synovial alpha-defensin (AD) (r = 0.759; p < 0.001). With a positive AD result, no additional PJI could be identified in any case.ConclusionAbsolute synovial PMN count is a widely available, rapid, cost-effective, and accurate marker in PJI diagnostics, whereas synovial AD appears to be a surrogate parameter of absolute synovial PMN count. Despite limitations in the early postoperative phase, wear, and rheumatic diseases in confirming PJI, an absolute synovial PMN count below 2,000/µl is highly suitable for ruling out PJI, with specific cut-off values for THA and TKA.Cite this article: Bone Joint J 2023;105-B(4):373–381.
Neurological recovery after early versus delayed surgical decompression for acute traumatic spinal cord injury
Authors: Hosman Allard J. F. , Barbagallo Giuseppe , van Middendorp Joost J. 
Published: March 2023

AimsThe aim of this study was to determine whether early surgical treatment results in better neurological recovery 12 months after injury than late surgical treatment in patients with acute traumatic spinal cord injury (tSCI).MethodsPatients with tSCI requiring surgical spinal decompression presenting to 17 centres in Europe were recruited. Depending on the timing of decompression, patients were divided into early (≤ 12 hours after injury) and late (> 12 hours and < 14 days after injury) groups. The American Spinal Injury Association neurological (ASIA) examination was performed at baseline (after injury but before decompression) and at 12 months. The primary endpoint was the change in Lower Extremity Motor Score (LEMS) from baseline to 12 months.ResultsThe final analyses comprised 159 patients in the early and 135 in the late group. Patients in the early group had significantly more severe neurological impairment before surgical treatment. For unadjusted complete-case analysis, mean change in LEMS was 15.6 (95% confidence interval (CI) 12.1 to 19.0) in the early and 11.3 (95% CI 8.3 to 14.3) in the late group, with a mean between-group difference of 4.3 (95% CI -0.3 to 8.8). Using multiply imputed data adjusting for baseline LEMS, baseline ASIA Impairment Scale (AIS), and propensity score, the mean between-group difference in the change in LEMS decreased to 2.2 (95% CI -1.5 to 5.9).ConclusionCompared to late surgical decompression, early surgical decompression following acute tSCI did not result in statistically significant or clinically meaningful neurological improvements 12 months after injury. These results, however, do not impact the well-established need for acute, non-surgical tSCI management. This is the first study to highlight that a combination of baseline imbalances, ceiling effects, and loss to follow-up rates may yield an overestimate of the effect of early surgical decompression in unadjusted analyses, which underpins the importance of adjusted statistical analyses in acute tSCI research.Cite this article: Bone Joint J 2023;105-B(4):400–411.
Continuous improvement in optimizing the timing of axial, hip, and femoral fracture fixation
Authors: Vallier Heather A. 
Published: March 2023

Benefits of early stabilization of femoral shaft fractures, in mitigation of pulmonary and other complications, have been recognized over the past decades. Investigation into the appropriate level of resuscitation, and other measures of readiness for definitive fixation, versus a damage control strategy have been ongoing. These principles are now being applied to fractures of the thoracolumbar spine, pelvis, and acetabulum. Systems of trauma care are evolving to encompass attention to expeditious and safe management of not only multiply injured patients with these major fractures, but also definitive care for hip and periprosthetic fractures, which pose a similar burden of patient recumbency until stabilized. Future directions regarding refinement of patient resuscitation, assessment, and treatment are anticipated, as is the potential for data sharing and registries in enhancing trauma system functionality.Cite this article: Bone Joint J 2023;105-B(4):361–364.
Which treatment provides the best neurological outcomes in acute spinal cord injury?
Authors: Birch Nick C. , Cheung Jason P. Y. , Takenaka Shota , El Masri Wagih S. 
Published: March 2023

Initial treatment of traumatic spinal cord injury remains as controversial in 2023 as it was in the early 19th century, when Sir Astley Cooper and Sir Charles Bell debated the merits or otherwise of surgery to relieve cord compression. There has been a lack of high-class evidence for early surgery, despite which expeditious intervention has become the surgical norm. This evidence deficit has been progressively addressed in the last decade and more modern statistical methods have been used to clarify some of the issues, which is demonstrated by the results of the SCI-POEM trial. However, there has never been a properly conducted trial of surgery versus active conservative care. As a result, it is still not known whether early surgery or active physiological management of the unstable injured spinal cord offers the better chance for recovery. Surgeons who care for patients with traumatic spinal cord injuries in the acute setting should be aware of the arguments on all sides of the debate, a summary of which this annotation presents.Cite this article: Bone Joint J 2023;105-B(4):347–355.
The impact of cement fixation on early mortality in arthroplasty for hip fracture
Authors: Ramsay Niamh , Close Jacqueline C. T. , Harris Ian A. , Harvey Lara A. 
Published: March 2023

AimsCementing in arthroplasty for hip fracture is associated with improved postoperative function, but may have an increased risk of early mortality compared to uncemented fixation. Quantifying this mortality risk is important in providing safe patient care. This study investigated the association between cement use in arthroplasty and mortality at 30 days and one year in patients aged 50 years and over with hip fracture.MethodsThis retrospective cohort study used linked data from the Australian Hip Fracture Registry and the National Death Index. Descriptive analysis and Kaplan-Meier survival curves tested the unadjusted association of mortality between cemented and uncemented procedures. Multilevel logistic regression, adjusted for covariates, tested the association between cement use and 30-day mortality following arthroplasty. Given the known institutional variation in preference for cemented fixation, an instrumental variable analysis was also performed to minimize the effect of unknown confounders. Adjusted Cox modelling analyzed the association between cement use and mortality at 30 days and one year following surgery.ResultsThe 30-day mortality was 6.9% for cemented and 4.9% for uncemented groups (p = 0.003). Cement use was significantly associated with 30-day mortality in the Kaplan-Meier survival curve (p = 0.003). After adjusting for covariates, no significant association between cement use and 30-day mortality was shown in the adjusted multilevel logistic regression (odd rati0 (OR) 1.1, 95% confidence interval (CI) 0.9 to 1.5; p = 0.366), or in the instrumental variable analysis (OR 1.0, 95% CI 0.9 to 1.0, p=0.524). There was no significant between-group difference in mortality within 30days (hazard ratio (HR) 0.9, 95% CI 0.7to 1.1; p = 0.355) or one year (HR 0.9 95% CI 0.8 to 1.1; p = 0.328) in the Cox modelling.ConclusionNo statistically significant difference in patient mortality with cement use in arthroplasty was demonstrated in this population, once adjusted for covariates. This study concludes that cementing in arthroplasty for hip fracture is a safe means of surgical fixation.Cite this article: Bone Jt Open 2023;4(3):198–204.
Parallel beam splitting and controlling system based on cascaded acousto-optic modulators
Authors: Cheng Wenhao , Zhu Guangzhi , Tang Haogang , Xu Puchang , Li Zhuoyun , Wu Minglang , Aleksei Kozlov , Zhu Xiao 
Published: March 2023

In recent years, ultrashort pulse lasers (lasers) have been already widely used for providing excellent laser machining quality for the electronics industry, replication tools, and other applications. However, the major drawback of this processing is low efficiency, especially for a large number of laser ablation demands. In this paper, a beam-splitting approach based on cascaded acousto-optic modulators (AOMs) is proposed and analyzed in detail. The cascaded AOMs can split a laser beam into several beamlets with the same propagation direction. These beamlets can be switched on and off individually, and the beam pitch can be changed independently. At the same time, the experimental setup of three cascaded AOM beam splittings is built up to verify the capability of the high-speed control (switching rate:1 MHz), high-energy utilization rate (>96% at three AOMs), and high-energy splitting uniformity (nonuniformity: 3.3%). This scalable approach enables the processing of arbitrary surface structures with high quality and efficiency.