bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fast, safe and high-throughput Real-Time PCR protocol for molecular sex identification inSalmo salar, applicable to historic scale collections
Authors: Anne-Laure Besnard , Lisa Meslier , Thibaut Jousseaume , Marie Nevoux , Frédéric Marchand , Sophie Launey 
Published: September 2023

AbstractConservation and management of wild species needs a detailed understanding of their population structure and dynamics. For instance, there are increasing evidence for sex-specific life history trajectories, even in species with limited morphological sexual dimorphism. In these species, identifying the sex of individuals in some or all life stages is a methodological challenge, which requires the development of new tools. Building on recent findings in molecular biology, we developed a new protocol for sex identification in Atlantic salmon (Salmo salar), aiming at the following criteria: accurate sex identification, fast diagnostic, applicable to large sample size, applicable to degraded DNA extracted from e.g. fish scales, and safe for the operator by limiting health and safety hazard. The protocol relies on multiplex Real-Time PCR using a set of specific primers. We tested its accuracy on a test set of 90 DNA samples from individuals of known morphological sex, and demonstrated its applicability on a large DNA sample from 384 individuals of unknown sex.
Fractionated irradiation of murine salivary glands resulted in focal acinar cell atrophy, immune cell infiltration, fibrosis, and hyposalivation
Authors: Juvkam Inga Solgård , Zlygosteva Olga , Malinen Eirik , Edin Nina Jeppesen , Galtung Hilde Kanli , Søland Tine Merete 
Published: September 2023

AbstractBackgroundRadiotherapy of head and neck cancer may cause detrimental late side effects such as fibrosis and hyposalivation. Our aim was to investigate late radiation-induced cellular and molecular changes of the salivary glands after fractionated irradiation to the head and neck in a murine model.Methods12-week-old female C57BL/6J mice were irradiated with X-rays to a total dose of 66 Gy, given in 10 fractions over 5 days. The radiation field covered the oral cavity and major salivary glands. Salivary gland function was assessed by collecting saliva at baseline and at various time points after irradiation. The submandibular (SMG), sublingual (SLG), and parotid glands (PG) were dissected at day 105. Using different staining techniques, morphological, cellular, and molecular changes were investigated in the salivary glands.ResultsSaliva production was significantly reduced in irradiated compared to control mice at day 35, 80, and 105. We observed a significant decrease in total gland area and a significant increase in fibrotic area in irradiated compared to control SMG at day 105. Atrophy of acinar cells was observed in all irradiated SMG and SLG. Increased amount of chronic inflammatory cells, increased cell proliferation and altered expression of apoptotic markers were found in atrophic areas of irradiated glands.ConclusionAcinar and duct cells in irradiated salivary glands show increased cell proliferation and altered expression of apoptotic markers, proposing an attempt to overcome or withstand tissue damage caused by irradiation. This suggests a potential for regeneration of salivary glands after radiation therapy.
RGG-motif protein Scd6 affects oxidative stress response by regulating Cytosolic caTalase T1 (Ctt1)
Authors: Tiwari Sweta , Sj Sudharshan , Rajyaguru Purusharth I 
Published: September 2023

AbstractIn response to stress, cells undergo gene expression reprogramming to cope with external stimuli. As translation is energy consuming process, its regulation during stress is crucial for cellular adaptation. Cells utilize a conserved stress response mechanism called global downregulation of translation, leading to the storage of translationally repressed mRNAs in RNA granules or RNP condensates. During oxidative stress induced by H2O2, genes responsible for combating oxidative stress, such as catalases and glutathione peroxidase, are strongly induced. However, the post-transcriptional regulatory events affecting these genes during H2O2stress are not well-explored. RNA binding proteins such as RGG motif proteins play a critical role in mediating translation regulation and have diverse physiological functions. Scd6, an RGG motif-containing protein in yeast, acts as a translational repressor through its interaction with eIF4G. Although the role of Scd6 as a translational repressor and decapping activator is known, its specific mRNA targets are largely unknown.This study identifies the role of Scd6 in oxidative stress response by regulating cytoplasmic catalaseT1 (CTT1). Altering Scd6 levels influenced Ctt1 protein levels, thereby affecting oxidative stress response. Scd6 overexpression increased sensitivity, while deletion enhanced tolerance to H2O2treatment due to ROS accumulation in the yeast cell. In response to H2O2treatment, Scd6 forms dynamic puncta which contains RNA. Overall, this study proposes regulation of oxidative stress response via modulation ofCTT1mRNA by Scd6.
Motor cortex is required for flexible but not automatic motor sequences
Authors: Mizes Kevin G. C. , Lindsey Jack , Escola G. Sean , Ölveczky Bence P. 
Published: September 2023

AbstractHow motor cortex contributes to motor sequence execution is much debated, with studies supporting disparate views. Here we probe the degree to which motor cortex’s engagement depends on task demands, specifically whether its role differs for highly practiced, or ‘automatic’, sequences versus flexible sequences informed by external events. To test this, we trained rats to generate three-element motor sequences either by overtraining them on a single sequence or by having them follow instructive visual cues. Lesioning motor cortex revealed that it is necessary for flexible cue-driven motor sequences but dispensable for single automatic behaviors trained in isolation. However, when an automatic motor sequence was practiced alongside the flexible task, it became motor cortex-dependent, suggesting that subcortical consolidation of an automatic motor sequence is delayed or prevented when the same sequence is produced also in a flexible context. A simple neural network model recapitulated these results and explained the underlying circuit mechanisms. Our results critically delineate the role of motor cortex in motor sequence execution, describing the condition under which it is engaged and the functions it fulfills, thus reconciling seemingly conflicting views about motor cortex’s role in motor sequence generation.
Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection inCaenorhabditis elegans
Authors: Vassallo Brian G. , Scheidel Noémie , Fischer Sylvia E. J. , Kim Dennis H. 
Published: September 2023

AbstractThe microbiota is a key determinant of the physiology and immunity of animal hosts. The factors governing the transmissibility of viruses between susceptible hosts are incompletely understood. Bacteria serve as food forCaenorhabditis elegansand represent an integral part of the natural environment ofC. elegans.We determined the effects of bacteria isolated withC. elegansfrom its natural environment on the transmission of Orsay virus inC. elegansusing quantitative virus transmission and host susceptibility assays. We observed thatOchrobactrumspecies promoted Orsay virus transmission, whereasPseudomonas luridaMYb11 attenuated virus transmission relative to the standard laboratory bacterial foodEscherichia coliOP50. We found that pathogenicPseudomonas aeruginosastrains PA01 and PA14 further attenuated virus transmission. We determined that the amount of Orsay virus required to infect 50% of aC. eleganspopulation onP. luridaMYb11 compared withOchrobactrum vermisMYb71 was dramatically increased, over three orders of magnitude. Host susceptibility was attenuated even further in presence ofP. aeruginosaPA14. Genetic analysis of the determinants ofP. aeruginosarequired for attenuation ofC. eleganssusceptibility to Orsay virus infection revealed a role for regulators of quorum sensing. Our data suggest that distinct constituents of theC. elegansmicrobiota and potential pathogens can have widely divergent effects on Orsay virus transmission, such that associated bacteria can effectively determine host susceptibility versus resistance to viral infection. Our study provides quantitative evidence for a critical role for tripartite host-virus-bacteria interactions in determining the transmissibility of viruses among susceptible hosts.
Phytohormones regulate asexualToxoplasma gondiireplication
Authors: Wagner Tina , Bangoura Berit , Wiedmer Stefanie , Daugschies Arwid , Dunay Ildiko Rita 
Published: September 2023

AbstractThe protozoanToxoplasma (T.) gondiiis a zoonotic disease agent causing systemic infection in warm-blooded intermediate hosts including humans. During acute infections, the parasite infects host cells and multiplies intracellularly in the asexual tachyzoite stage. In this stage of the life cycle, invasion, multiplication, and egress are most critical events in the parasite’s replication.T. gondiifeatures diverse cell organelles to support these processes, including the so-called apicoplast, an endosymbiont-derived vestigial plastid originating from an alga ancestor. Previous studies have highlighted that phytohormones can modify the calcium-mediated secretion, e.g., of adhesins involved in parasite movement and cell invasion processes. The present study aimed to elucidate the influence of different plant hormones on the replication of asexual tachyzoites in a human foreskin fibroblast (HFF) host cell culture.T. gondiireplication was measured by the determination ofT. gondiiDNA copies via qPCR. Three selected phytohormones, namely abscisic acid (ABA), gibberellic acid (GIBB), and kinetin (KIN)as representatives of different plant hormone groups were tested. Moreover, the influence of typical cell culture media components on the phytohormone effects was assessed. Our results indicate that ABA is able to induce a significant increase ofT. gondiiDNA copies in typical supplemented cell culture medium when applied in concentrations of 20 ng/μl or 2 ng/μl, respectively. In contrast, depending on the culture medium composition, GIBB may potentially serve asT. gondiigrowth inhibitor and may be further investigated as a potential treatment for toxoplasmosis.
Systematic investigation of the logistics service quality of cross-border e-commerce: a mixed-methods perspective
Authors: Zhang Yu , Yuan Yafen , Su Jiafu 
Published: September 2023

PurposeThis study explores the factors that characterize the logistics service quality (LSQ) of cross-border e-commerce and identifies the different relationships between these factors with respect to customer satisfaction.Design/methodology/approachThe study applied a two-stage mixed-methods design. The first stage (Stage 1) was a qualitative study of 3,000 reviews from the Amazon China e-commerce platform. The second stage (Stage 2) included a quantitative study that analyzed survey data from 590 Chinese cross-border e-commerce customers using the Kano model.FindingsStage 1 involved developing a conceptual framework for the LSQ of cross-border e-commerce, including six dimensions: timeliness, safety, reliability, economy, personnel contact quality and information quality. In Stage 2, the study found that only reliability and personnel contact quality indicators are linearly related to customer satisfaction. Timeliness and the safety of packaging greatly contribute to customer satisfaction, but do not cause dissatisfaction when unfulfilled. Economics and information quality indicators, and the safety of goods, are basic requirements that tend to provoke customer dissatisfaction when unmet, but do not increase customer satisfaction when they are met.Originality/valueThis study is one of the first to construct a conceptual model of LSQ that applies to cross-border e-commerce and to identify the instrumental nature of various LSQ attributes and their impact on improved customer satisfaction.
Bibliometric and content analyses of research on technology use in real estate facilities management
Authors: Okoro Chioma 
Published: September 2023

Purpose
Technological change drives transformation in most sectors of the economy. Industry 4.0 technologies have been applied at different stages of a building’s lifecycle. However, limited studies exist on their application in real estate facilities management (REFM). This study aims to assess the existing knowledge on the topic to suggest further research directions.

Design/methodology/approach
Scopus-indexed literature from 2013 to 2023 was examined and visualised using VOSviewer software to output quantitative (descriptive) results. Content analysis was used to complement the quantitative findings.

Findings
Findings indicated a concentration of research in China, Norway and Italy. The knowledge areas included three clusters: lifecycle integration and management, data curation and management and organisational and management capabilities. The benefits, challenges and support strategies were highlighted.

Research limitations/implications
More collaboration is needed across countries and territories on technology integration in REFM. Future research using alternative methodologies is recommended, with a focus on adopting and non-adopting REFM organisations. Further, implications for facility managers, employees, technology suppliers or vendors, training, organisations and management exist.

Practical implications
Further, implications for facility managers, employees, technology suppliers or vendors, training, organisations and management exist.

Originality/value
The study reveals the knowledge base on technology use in REFM. It adds to the evidence base on innovation and technology adoption in REFM.
The impact of normative institutions on socially sustainable supply chain management: the role of individual cultural values
Authors: Zhang Chi , Venkatesh Mani , Ohana Marc 
Published: September 2023

PurposeDrawing on institutional theory, this study investigates the role of individual cultural values on the adoption of socially sustainable supply chain management (socially SSCM) for Chinese suppliers facing the normative institutional pressures of guanxi (interpersonal relationships).Design/methodology/approachUsing empirical data collected in three waves from 205 Chinese manufacturers supplying international markets, the proposed theoretical model is tested through partial least squares structural equation modeling (PLS-SEM).FindingsThe results indicate that guanxi has a positive impact on socially SSCM, and this positive effect is strengthened when the individual cultural values of the supplier's representative embody high collectivism and low uncertainty avoidance.Research limitations/implicationsThis study highlights the leading role of guanxi in improving socially SSCM practices due to its normative institutional force. In addition, the findings suggest that future studies should consider individual differences in supply chain partners, which may lead to distinct reactions when facing normative institutional pressures.Practical implicationsThis study suggests international buyers should adopt guanxi management with their Chinese suppliers to encourage them to adopt socially SSCM. In addition, managers should note that the guanxi strategy is more effective when the supplier's representative collectivism is high and uncertainty avoidance is low.Originality/valueThis study contributes to socially SSCM research in emerging economies by unveiling the role of guanxi as a key driver of socially SSCM in the Chinese market and providing empirical evidence of the moderating effect of individual culture on the guanxi normative institutionalization process.
More on adoption criteria of revolutionary business techniques: the case of ETFs
Authors: Brockhoff Klaus 
Published: September 2023

Purpose
This paper aims to add further evidence to adoption criteria for “revolutionary” business techniques.

Design/methodology/approach
Adoption criteria for business techniques with a high degree of novelty have been developed earlier. The case of exchange-traded funds supports the earlier findings. The methodology applied is explicative.

Findings
The analysis supports findings that an effective response to a problem, the availability of a controllable procedure, the means to apply the procedure easily and the hardware jointly explain adopting “revolutionary” business techniques.

Research limitations/implications
The results of case studies, in general, do not permit induction. More research might identify additional adoption criteria or falsify the presently obtained results. Therefore, further research is invited.

Practical implications
Managers seeking or being introduced to new techniques in business administration might use the criteria outlined here for their evaluation.

Originality/value
The author believes this paper corroborates earlier findings on adopting “revolutionary” business techniques that draw on theoretically developed technologies.
Quality standards for tribunal decision making in strata disputes
Authors: Knoll A.M. David D. 
Published: September 2023

Purpose
This study aims to investigate Australian civil tribunal decisions to ascertain compliance with decisional quality standards in Australian law, with a particular focus on strata and community title cases.

Design/methodology/approach
An orthodox doctrinal legal analysis and assessment of cases and tribunal policies was adopted. All Australian jurisdictions were surveyed, including federal, state and territory jurisdictions. The case law in each jurisdiction was screened to identify whether the principles applicable to decisional quality were engaged and then analysed as to the extent of that engagement.

Findings
Where a party presents a substantial, clearly particularised argument relying upon established facts, tribunals are obliged to address those facts and the arguments by way of an active intellectual process. However, appellate decisions disclose a degree of deference not often accorded to judicial officers, and there is a need for a more disciplined approach to ascertain whether any errors have been made by a tribunal lie on the critical path to the decision. As strata and community title disputes become more complex, the importance of decisional quality standards can only increase.

Research limitations/implications
Up to date as of 1 March 2023.

Practical implications
The present position would appear to be that where a party presents a substantial, clearly particularised argument relying upon established facts, a tribunal must address its mind to those facts and the arguments by way of an active intellectual process. The requirement is limited to circumstances prescribed by a statute and factual and legal issues which are necessary to be determined in order for the tribunal to be satisfied as to circumstances prescribed by a statute. However, where the errors are not gross and plainly obvious, appeals from defective tribunal decisions are unlikely to succeed. There is a degree of deference not often accorded to judicial officers. That deference is unfortunate when tribunals are allocated jurisdiction over what quite often are significant property disputes.

Social implications
The impact on community living of uncorrected poor quality tribunal decisions can be immense, depending on the degree of error. For example, water ingress into people’s homes might remain unremedied for many years, as, for example, occurred in the Marinko case.

Originality/value
The research and analysis is entirely original. A search of journals and textbooks did not identify any prior analysis, at least in the Australian context, relating to decisional quality standards of tribunals.
Contagion in the Euro area sovereign CDS market: a spatial approach
Authors: Ben Abdallah Nadia , Dabbou Halim , Gallali Mohamed Imen 
Published: September 2023

PurposeThis paper explores whether the Euro-area sovereign credit default swap market is prone to contagion effects. It investigates whether the sharp increase in sovereign CDS spread of a given country is due to a deterioration of the macroeconomic variables or some form of contagion.Design/methodology/approachFor this purpose, the authors use an innovative approach, i.e. spatial econometrics. Although modeling spatial dependence is an attractive challenge, its application in the field of finance remains limited.FindingsThe empirical findings show strong evidence of spatial dependence highlighting the presence of pure contagion. Furthermore, evidence of wake-up call contagion-increased sensitivity of investors to fundamentals of neighboring countries and shift contagion-increased sensitivity to common factors are well recorded.Originality/valueThis study aims to study a crucial financial issue that gained increased research interest, i.e. financial contagion. A methodological contribution is made by extending the standard spatial Durbin model (SDM) to analyze and differentiate between several forms of contagion. The results can be used to understand how shocks are spreading through countries.
Advertising and financial performance: insight from a competitive market in Africa
Authors: Akorede Hamza 
Published: September 2023

Purpose
This study aims to examine the relationship between advertisement expenditure and firm performance as well as the moderating effects of firm age and size on this relationship.

Design/methodology/approach
Twenty-eight selected companies listed on the Nigerian stock exchange were examined. The study used multiple regression, a quantitative research method, to capture both the direct and moderating effects.

Findings
The findings show that advertisement has a positive relationship with sales but an insignificant relationship with return on asset. Furthermore, the results indicate that larger firms outperform smaller ones when using advertisements to enhance their sales. On the contrary, there is no significant difference between the use of advertisement by young and older firms in improving financial performance.

Originality/value
Due to the often-wrong use of resource base view in the advertisement–performance relationship and contradiction in research findings, this paper re-conceptualize advertisement as a necessary investment (just like plant and equipment) but not an investment that provide strategic value. The paper also makes novel argument by theorizing a negative relationship between advertisement and firms’ performance in the Nigerian context.