bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors associated with influenza vaccination rates by vaccination government financial support policy: A cross sectional study (KNHNES 2020)
Authors: Park Sunhee , Kwon Jeoung A , Kim Hyo-Seon , Kim Byungmi 
Published: March 2023

Abstract
Objectives To investigate factors associated with influenza vaccination rates by vaccination government financial aid policy
Methods We used the Korean National Health and Nutrition Examination Survey (2020), a nationally representative, cross-sectional, population-based study. A total of 5,582 participants were included. Multivariable logistic regression was performed to analyze the effect on vaccination rate after adjusting for socioeconomic characteristics.
Results Financial aid group (71.2%) is higher vaccinated than non-aid group (33.9%) in crude rate. In the both group low education levels showed significant relationships with the financial aid group (aOR 2.28; CI: 1.41–3.70) and the non-aid group (aOR 1.86; CI: 1.14–3.05). In not receiving support group, high income was significantly related to high vaccination rates (aOR 2.23; 95% CI: 1.45–3.44). Women showed high, self-employed health insurance type showed low vaccination rates. And occupation type showed significant relationships in only financial support group.
Conclusion The vaccination rate had significant association by government financial support. In the future, to efficiently use limited resources, implementing strategic policies targeting appropriate factors for each group may be appropriate.
Niche differentiation and influencing factors of nitrite oxidation bacteria Nitrospira in sediments of the Luan River estuary in China
Authors: Zhao Jiawei , Guan Xiangyu , Shi Xiaonan , Guo Wei , Luo Ximing 
Published: March 2023

Abstract
In estuarine sediment, the nitrite oxidation process mediated by bacteria is significant in the nitrification process. Nitrospira is the most widely distributed in diverse group of nitrite-oxidizing bacteria (NOB), and can adapt to various environment. In this study, the Nitrospira-specific primer nxrB 169F-638R was used to analyze the microbial communities in the sediments of low-, middle-, and high-level zones of the Luan River estuary. The structure of microbial community and its response to environmental factors were discussed. The abundance and diversity of Nitrospira were the highest in the low-level zone and the lowest in the high-level zone. Lineage II (43.58%) and lineage IV (32.09%) were the dominant Nitrospira. It was found that the distribution pattern of Nitrospira was also affected by complex environmental factors such as the concentration of NH4+, Fe and Cu cations. Therefore, this study is helpful in understanding the niche differentiation and adaptation strategies of Nitrospira in an estuarine sediment environment and in providing references for single-step nitrification.
Accuracy and precision of the certified Glucometer-Strips System Contour Plus One in clinical practice
Authors: Chlup Rudolf , Zálešáková Hana , Doubravová Blanka , Ďurajková Emilia , Nezval Martin , Gottwaldová Jiřina , Zapletalová Jana 
Published: March 2023

Abstract
Aims 1. To revise the accuracy and precision of the glucometer-strips system (GSS) in a professional routine. 2. To support the feasibility of capillary plasma glucose (cPG) monitoring.
Methods Prospective single-center study. Triplets of cPG were investigated on GSS Contour Plus One (Ascensia, Switzerland) using glucosedehydrogenase strips. To obtain one triplet, one LOT of strips was used in three glucometers Contour Plus One. Following the cPG readings, venous blood was taken into a Grainer tube to measure venous plasma glucose (vPG) on a Cobas Integra 400 analyzer using hexokinase. IBM SPSS Statistics for Windows, V. 23.0 was applied. A total of 258 pairs of cPG vs vPG were compared using the Wilcoxon signed rank test with Bonferroni correction. P<0.05 was considered significant. Precision was estimated using the SD of average differences between individual cPG measurements.
Results Deviations of cPG measured on GSS Contour Plus One from vPG (in range ≥5.55 mmo/L) measured on Cobas Integra Analyzer were -0.63±0.71 mmol/L (mean±SD). The number of cPG values measured on Contour Plus One within the required interval ±15% of vPG values was 200/207 (96.6%). For vPG <5.55 mmol/L, the required interval of ±0.83 mmol/L was reached in 51/51 (100%) cPG values measured on GSS Contour Plus One. PG precision/variability in triplets of GSS Contour Plus One was demonstrated by SD 0.204±0.247 mmol/L.
Conclusions The required accuracy and precision of GSS Contour Plus One in clinical practice was confirmed and corresponds to the Requirements for blood-glucose monitoring systems for self-testing in managing diabetes mellitus ISO 15197 (2015). In addition to sustainable reviews of registered GSS, experienced data communication between health care professionals and GSS users should also be considered.
Identification and Validation of Cuproptosis-Related LncRNA Signatures as a Novel Prognostic Model for Wilms Tumor
Authors: Chang Mengmeng , Gao Hongjie , Liu Xiaoya , Ma Wenyue , Jia Changlin , Sun Fengyin 
Published: March 2023

Abstract
Background: Wilms tumor (WT), which is the most common malignancy of the genitourinary system in children. Cuproptosis is a unique form of cell death, and the mechanism of interaction with WT is not yet clear. This study aims at using bioinformatics technology to construct a prognostic signature and identify cuproptosis-related biomarkers to improve the prognosis and treatment of WT.
Methods: RNA sequencing and clinical data were downloaded from the TARGET database. The cuproptosis-related lncRNAs were screened by Pearson's correlation calculation, the differentially expressed cuproptosis-related lncRNAs were screened by R software, and a univariate Cox regression analysis was performed on them to screen out the lncRNAs related to prognosis. The least absolute shrinkage and selection operator (LASSO) Cox regression analysis was performed to construct a cuproptosis-related lncRNA signature. The predictive ability of the signature was then assessed by Kaplan-Meier (KM) curves, clinically relevant subject acceptance characteristic (ROC) curves, decision curves (DCA), and nomograms. Other clinical factors were further included to verify whether the constructed prognostic signature could be an independent predictor. GO and KEGG functional enrichment analysis was performed on the target lncRNA-related mRNAs to explore their possible functions and pathways involved. Immune infiltration and tumor microenvironment were assessed by CIBERSORT, ESTIMATE, and ssGSEA algorithms to explore potential links between cuproptosis-related lncRNAs related to prognosis.
Results: ①A total of 711 cuproptosis-related lncRNAs were screened. ②Through univariate Cox regression analysis, Lasso regression, and multivariate Cox regression analysis, a total of 17 lncRNAs with independent prognostic significance were screened to construct a prognostic signature. ③ The KM survival analysis of the samples by the prognostic signature found that there was a significant difference in survival between the high and low-risk groups, and the high-risk group had a worse prognosis (P<0.01). The area under the time-dependent ROC curve at 1, 3, and 5 years was 0.903, 0.882, and 0.907, combined with other clinical traits to construct the combined index ROC curve, the area under the risk score curve of the prognostic signature was 0.93, indicating that it can be used as an independent prognostic factor independently of other clinical traits.
Conclusions: The 17 cuproptosis-related lncRNAs and their prognostic risk signature may be molecular biomarkers and therapeutic targets for the prognosis of Wilms tumor.
Integrating ATAC-seq and RNA-seq to identify differentially expressed genes with chromatin-accessible changes during photosynthetic establishment in Populus leaves
Authors: Zhang Sheng-Ying , Zhao Biligen-Gaowa , Shen Zhuo , Mei Ying-Chang , Li Guo , Zhang Jiao , Chao Qing , Wang Bai-Chen 
Published: March 2023

Abstract
Leaves are the main photosynthetic organs to provide substance for secondary growth in woody plants. It is important to obtain an anatomical understanding and regulatory network analysis of leaf development.We first observed serial leaves of poplar anatomically using semi-thin sections, transmission electron microscopy, and scanning electron microscopy, and performed RNA-seq analysis. We found that the third leaf possesses the anatomy required for photosynthesis and the first to third leaves to represent a critical period for leaf development. Transcription factors bind to accessible promoter regions in chromatin and precisely regulate the expression of their target genes. Therefore, we selected the first and third leaves to use an assay for transposase‐accessible chromatin using sequencing (ATAC-seq) combined with RNA-seq to identify differentially expressed genes (DEGs) and their differentially accessible regions (DARs) during leaf development. We identified 390 and 345 DEGs in which DARs in promoter presented as more open (i.e. gain DARs and loss DARs) in the third and the first leaf, respectively. Meanwhile, we performed a motif enrichment analysis and found 17 and 15 upstream transcription factors (TFs) in the gain DARs and loss DARs. Furthermore, we could access the regulatory network of leaf development, which lays the molecular foundation for future understanding of leaf photosynthesis.
Comparing the pregnancy outcomes of fresh and frozen embryo transfer after early rescue ICSI
Authors: JIANG YAN , YUAN JING-CHUAN , SONG GE , ZHANG XU-HUI , MIAO SUI-BING , WU XIAO-HUA 
Published: March 2023

Abstract
Context: Early-rescue intracytoplasmic sperm injection (R-ICSI) can avoid total fertilization failure in conventional in vitro fertilization (IVF). However, the R-ICSI embryos had lower implantation rate than the direct ICSI in the fresh embryo transfer.
Aims: To investigate the effect of frozen embryo transfer (FET)after R-ICSI.
Methods: This was a retrospective study of the first cycle primary infertility patients with the age ≤ 35 undergoing R-ICSI and ICSI treatment. The clinical pregnancy rate, implantation rate, ectopic pregnancy, abortion rate and live birth rate were analyzed between the R-ICSI and ICSI groups in their first embryo transfer (fresh and FET cycles).
Key Results: The average age of patients in fresh and frozen ET of two groups was (29.1±3.1 vs. 29.0±3.2, and 28.9±3.0 vs. 29.1±3.3), respectively (P>0.05). The R-ICSI embryos clinical pregnancy rate, implantation rate and live birth rate were lower than the ICSI embryos in fresh embryo transfer. Whereas there were no significant difference between R-ICSI and ICSI embryos in the FET cycle.
Conclusions: R-ICSI embryos with frozen embryo transfer would be an optimal strategy rather than fresh embryo transfers.
Implications:early R-ICSI combined with frozen embryo transfer could achieve satisfying clinical outcomes compared with fresh embryo transfers.
Potential limits on warming-enhanced nitrogen availability in mesic boreal forests
Authors: Ziegler Susan E , Podrebarac Frances A , Billings Sharon A , Edwards Kate A , Skinner Andrea , Buckeridge Kate M , VandenBoer Trevor C 
Published: March 2023

Abstract
In mesic regions, where water availability is rarely limiting, temperature increases with climate change can prompt increases in primary production. These responses will be determined in part by nitrogen (N) and phosphorus (P) availability, yet the relative importance of N- versus P-cycling for supporting primary production and soil C is unclear. Prompted by previous studies across a mesic boreal forest transect, we hypothesized that enhanced N-cycling and availability supporting increased productivity in warmer forests results in greater relative P-limitation. By assessing soil N and P stocks and inputs across this transect we uncovered multiple lines of evidence supporting this hypothesis. First, soil N stocks and soil N:P are highest in the warmer forests while soil C:P and P stocks are maintained across latitude. Second, soil N:P increases with depth in the warmest forests where organic P is the dominant form of P, and where the lowest mineral soil P stocks are observed. Third, estimates of soil P residence time are not coupled with those of C, and soil C:P does not correlate with tree growth, indicating that P availability is not supported by warming-induced microbial cycling, as is the case for N. Consistent with this, green needle litterfall C:P was lowest in the warmest sites suggesting enhanced P-resorption in those forests. These results provide nuanced evidence for relative P limitation that may emerge with the enhanced N cycling and productivity driven by a warmer climate suggesting a constraint on an important, negative climate feedback in these forests.
Scenario Planning in the Health by Trend Impact Analysis (TIA) Methodology
Authors: Alikhani Alireza , Golkar Mostafa Hosseini , Sharifi Hamid , Najafi Farid , Haghdoost AliAkbar 
Published: March 2023

Abstract
Background: In the health field, future planning and determining the road map are important issues. In this regard, quantitative approaches have often dominated this area of health sector research, and the main focus of researchers has been on creating frameworks and solutions for data management and applying data-driven strategies such as forecasting. in using such methods, it is assumed that past effective forces will continue under the same conditions in the future, as well as new events that They can change past relationships or distort trends, will not occur or have no significant effect. therefore, considering the influential events and investigating the significant effects of these events and their subsequent processes can lead to a better understanding of possible consequences and proper planning for futures in the field of health.
Methods: In the trend impact analysis approach, the impact of unprecedented events and their occurrence in the future on trends is considered and reliable results will be produced. the historical available data are used to perform quantitative extrapolation and forecast future values Then, qualitative tools and methods are employed to identify a list of influential events that will affect the trends in the future. Finally, the impact of these events on trends will be identified and applied.
Results: As a result of this approach, probable and plausible scenarios can be achieved.
Conclusions: The limitations in forecasting the future by using quantitative methods and ignoring the effect of unexpected events can leads to "surprises free" predictions and cause deviations from estimates and plans. Use of innovative methods in the methodology of studies will provide the ground for intelligent planning to face the future, and along with the complexities, uncertainties and changes in social behavior in societies, the use of new scientific methods in the field of health is a necessity.
D2 receptor sensitization: Neurobiological basis of increase sensitivity of stress on stimulant induced behavioural sensitization.
Authors: Alam Nausheen , Abbasi shagufta , Naeem Sadaf 
Published: March 2023

Abstract
Stress is associated with high risk of psychological disorders and cognitive impairment. Psychostimulants are known to enhanced cognition but prolong use causes behavioural sensitization. The locomotor response to psychostimulant potentiated after repeated exposure to stressful stimulus & dopamine play a major role in sensitization-like phenomena. The present study is conducted to visualize responsiveness of dopamine D2 receptors by challenge dose of haloperidol following long term psychostimulant administration in restrained & unrestrained rats. Behavioural Effects of oral methylphenidate (10mg/kg/day 2 times daily) & modafinil (75mg/kg/day once daily) administration in restrained (exposed to immobilization stress for 2 hours daily) and unrestrained rats were monitored for 35 days till the behavioral sensitization produced by using home cage (performed after every two days), open field, Head Dip Test and passive avoidance test (PAT) weekly. The effects of haloperidol challenge dose (1 mg/kg i.p.) were monitored after 35 days of treatment using Home Cage, Open Field, Head Dip and PAT, 30-, 60-, 90- & 120-minutes post injection. The result shows that restrained stress potentiate stimulant-induced behavioural sensitization, produced cognitive deficits and decrease exploratory activity but both stimulants improved cognitive performance and exploratory activity in PAT and head dip test respectively effect was more pronounced in modafinil treated rats. Challenge dose of haloperidol ameliorated motor activity in Home Cage and Open Field, impaired cognition in PAT and decreased activity in head dip test, effect was significant in psychostimulant treated rats but more pronounced in saline treated restrained and psychostimulant treated restrained rats which could be due to supersensitization of D2 receptors that leads to increased sensitivity of stress on stimulant induced behavioural sensitization. This study will help to investigate drugs that could prevent psychostimulant induced behavioural sensitization in stressful environment by understanding of neurobiological basis of the effect of psychostimulants on stress induced motor and cognitive deficits.
Structural Conservation of Insulin/IGF Signalling Axis at the Insulin Receptors Level in Drosophila and Humans
Authors: Viola Cristina , Frittmann Orsolya , Jenkins Huw , Shafi Talha , De Meyts Pierre , Brzozowski Andrzej 
Published: March 2023

Abstract
The family of insulin-related hormones regulates key life processes in Metazoa, from metabolism to growth, reproduction, lifespan and aging, through an evolutionarily conserved Insulin/insulin-like growth factor-1 (IGF-1) signalling (IIS) axis. In humans the IIS axis is controlled by insulin, two insulin-like growth factors, two isoforms of the insulin receptor (hIR-A and -B), its homologous IGF-1R and their hybrid dimers. In Drosophila, this signalling engages seven insulin-like proteins (DILP1-7) and a single receptor (dmIR), with many downstream proteins and pathways that are homologous with human ones. This report reveals the first cryoEM structure of the dmIR ectodomain:DILP5 complex providing the evidence of structural homology between dmIR and hIR. In the presence of excess hormone, the overall organisation of the dmIR complex is an asymmetric ‘T’ conformation, similar to that observed in some human IR structures. However, dmIR binds three DILP5 molecules in an unseen arrangement, revealing unique structural signatures that could rationalize the selective binding and signalling by different DILPs. This work provides structural proof of the evolutionary conservation of key receptors of the insulin-like hormones signalling axis, underpinning a deeper understanding of an important model animal organism.
Unmet supportive care needs of patients with breast cancer: A systematic review
Authors: Fan Rongrong , Wang Lili , Bu Xiaofan , Wang Wenxiu , Zhu Jing 
Published: March 2023

Abstract
Background
Growing breast cancer patients increases the demand for unmet needs. Few research has focused on any systematic research into unmet need among these groups. This aim is to clarify major elements of unmet needs and factors influencing in order to provide a basis for provision of appropriate medical services.
Method
A throughout search was conducted in Cochrane Library, PubMed, Embase, Web of Science, Medline from inception through December 2022. Studies investigated unmet supportive care needs of patients with breast cancer were included. Two independent researchers extracted the data, evaluated the quality of eligible studies, and performed double-checks. Methodological quality was independently appraised by two authors in accordance with JBI critical appraisal tools. Content analytic approach was applied to narrative synthesis. For quantitative studies, the meta-analysis was also performed to pool the proportions through Review Manager Software.
Results
Our present review identified 63 studies evidencing the unmet needs of patients with breast cancer. A total of 10534 records were retrieved and 63 studies were included finally. The overall risk of bias was low to moderate. Self-made questionnaire was the most used instrument, followed by The Short-form Supportive Care Needs Survey questionnaire (SCNS-SF34). Social support (74%), fear of cancer recurrence/ spreading (55%), daily activity (54%), sexual/intimacy (52%) and information support (50%) were the unmet needs with most proportion. Information need and psychological/emotional need were been found to appear most frequently. The most reported unmet needs among breast cancer patients were dignity (55.8%), physical/symptom (55%), and daily activity (52.7%). Meanwhile, in breast cancer survivors, peer communication (65.5%), daily activity (56.5%), medical counselling (52.2%), and fear of cancer recurrence/ spreading (52%) were frequently reported. It was found that unmet needs were significantly associated with many factors such as demographic data, treatment, disease status, quality of life, performance status and some psychological indicators.
Conclusion
Breast cancer patients are experiencing a large number of unmet needs in fear of cancer recurrence, daily activity, sexual/intimacy, psychology and information. Standard assessment tools that specifically to unmet needs among breast cancer patients were absent. Patients with different characteristics had various unmet support need, which emphasized the necessity to verified elaborate care plan for different patients. We identified some potential risk factors of unmet need. Data synthesis through meta-analysis to confirm the influencing factors are demanded.
Exposing military attacks in the 2022 Russia-Ukraine conflict using seismic array data
Authors: Dando Ben , Goertz-Allmann Bettina , Brissaud Quentin , Köhler Andreas , Schweitzer Johannes , Kværna Tormod 
Published: March 2023

Abstract
Seismometers are generally used by the research community to study local or distant earthquakes, but seismograms also contain critical observations from regional1,2 and global explosions3, which can be used to better understand conflicts and identify potential breaches of international law. The large overpressure generated by an explosion, shakes the Earth’s atmosphere and subsurface, and the resulting ground motion can be recorded by seismometers, while the infrasound signals that propagate through the atmosphere can be detected by microbarometers. While this technology is used by the International Monitoring System4 to monitor nuclear explosions as part of the Comprehensive Nuclear Test Ban Treaty, the detection and location of lower-yield military attacks requires a network of sensors much closer to the source of the explosions. Without dedicated sensor deployments, such networks are rarely available for conflict monitoring. Obtaining comprehensive and objective data that can be used to effectively monitor an active conflict zone therefore remains a significant challenge. We demonstrate how seismic waves generated by explosions in northern Ukraine can be recorded by a local network of seismometers and used to automatically identify individual attacks in close to real-time, providing an unprecedented view of an active conflict zone. Between February and November 2022, we observe over 1200 explosions from the Kyiv, Zhytomyr and Chernhiv provinces, providing accurate origin times, locations and magnitudes. We identify a range of seismo-acoustic signals associated with various types of military attacks, with the resulting catalogue of explosions far exceeding the number of publicly reported attacks5. Our results demonstrate that seismic data can be an effective tool for providing accurate and objective data in real-time from an ongoing conflict. We anticipate that the implementation of seismic-based monitoring techniques can provide invaluable information about potential breaches of international law.