bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physicochemical, Microbiological, and Sensorial Properties of Chickpea Yogurt Analogue Produced with Different Types of Stabilizers
Authors: Fazla Sofia Nadhirah Mohd , Marzlan Anis Asyila , Hussin Anis Shobirin Meor , Rahim Muhamad Hafiz Abd , Madzuki Iffah Nadhira , Mohsin Aliah Zannierah 
Published: September 2023

Abstract

There is a growing need for plant-based yogurts analogue that meet consumer demands in terms of texture and sensory qualities. Stabilizers are crucial in plant-based yogurt's physical properties which develop a thicker and creamier texture mimicking dairy yogurt. The addition of stabilizers helps to prevent syneresis. Thus, the study aims to evaluate the effect of pectin, corn starch, and locust bean gum (LBG) at different ratios on the physical, chemical, microbiological, and sensory properties of chickpea yogurts analogue (CYA). The concentration of stabilizer significantly influenced (p < 0.05) the proximate compositions, physicochemical and textural properties, and cell viability. A significant increase (p < 0.05) was observed in yogurt viscosity with the addition of corn starch and LBG at 1.0%. Firmness and consistency were improved in samples supplemented with 1.0% corn starch and commercial stabilizer. The sensory evaluation indicated that adding LBG at the ratio of 0.5% generated better preference among panelists in the appearance, color, and texture aspects despite commercial CYA showing significantly higher overall acceptability (p < 0.05) than other samples. The stabilizer's behavior significantly impacts the features of CYA which with 0.5% LBG received high consumer acceptance, which proves a good potential for CYA to be on the same shelf with other commercial yogurts analogue in the market.
Advantages of Cell Proliferation and Immune Regulation in CD146+NESTIN+ HUMSCs Obtained from Extremely Premature Infants: Insights from Single-Cell RNA Sequencing
Authors: huang peng , Qin Xiaofei , Fan Chuiqin , zhong Huifeng , Wang Manna , Chen Fuyi , Liao Maochuan , Zheng Nanpeng , Wang Hongwu , Lin Bingchun , Ma Lian 
Published: September 2023

AbstractBackgroundIn our prior study, we discovered that human umbilical cord Wharton’s Jelly derived mesenchymal stem cells (HUMSCs) obtained from extremely preterm infants demonstrated superior characteristics compared to term infants, particularly regarding cell proliferation, pluripotency, and cell damage repair ability. To explore the underlying heterogeneity between these cells further, we utilized single-cell RNA sequencing (scRNA-seq) to examine their transcriptional differences and potential molecular pathways involved in this heterogeneity.MethodsWe conducted scRNA-seq on HUMSCs obtained from three distinct gestational ages- 22+5 weeks, 28 weeks, and 39 weeks, respectively. To assist in the analysis, we employed the scRNA-seq data from two bone marrow mesenchymal stem cells (BMSCs) samples available in existing literature as reference datasets. Subsequently, we undertook bioinformatics analysis on the obtained transcriptomic data using the R programming language.ResultsUpon merging the five samples, we were able to identify a total of 17 cell subpopulations with high expression of fibroblast and MSC markers. The expression of CD146 was found to be significantly higher in HUMSCs as compared to BMSCs. Moreover, we observed higher expression of Nestin+ cells in premature HUMSCs. Cell cycle analysis revealed that the majority of HUMSCs were in the G2M phase, while BMSCs were mainly in the G1 phase. Pseudotime analysis showed that HUMSCs had a lower degree of differentiation compared to BMSCs, and this decreased with increasing gestational age. Custom gene set scoring analysis revealed that the cells expressed genes related to osteogenesis, chondrogenesis, adipogenesis, stemness, immunology, and vasculogenesis; with preterm HUMSCs displaying an immunological edge. Differential gene analysis and gene enrichment analysis indicated that CD146+Nestin+ HUMSC subpopulations displayed upregulation in immune regulation, cell proliferation-related gene expression, and gene regulatory pathways.ConclusionscRNA-seq analysis revealed differences between BMSCs and HUMSCs at both preterm and term infant. Specifically, the expression of CD146+ and Nestin+ cells was significantly higher in preterm HUMSCs, which may contribute to their advantages in immune regulation, cell proliferation-related gene expression, and regulatory pathways. These findings hold great significance in advancing our understanding of the molecular mechanisms of HUMSCs and their potential applications in disease treatment, transplantation, and regenerative medicine.
Enhanced bacterial cancer therapy delivering therapeutic RNA interference of c-Myc, a key driver of tumourigenesis.
Authors: Williams Jason S. , Higgins Adam T. , Stott Katie J. , Thomas Carly , Farrell Lydia , Bonnet Cleo , Peneva Severena , Derrick Anna V. , Hay Trevor , Wang Tianqi , Morgan Claire , Dwyer Sarah , D’Ambrogio Joshua , Hogan Catherine , Smalley Matthew J. , Parry Lee , Dyson Paul 
Published: September 2023

Abstract
BACKGROUND:
Bacterial cancer therapy was first trialled in patients at the end of the nineteenth century. More recently, tumour-targeting bacteria have been harnessed to deliver plasmid-expressed therapeutic interfering RNA to a range of solid tumours. A major limitation to clinical translation of this is the short-term nature of RNA interference in vivo due to plasmid instability. To overcome this, we sought to develop tumour-targeting attenuated bacteria that stably express shRNA by virtue of integration of an expression cassette within the bacterial chromosome and demonstrate therapeutic efficacy in vitro and in vivo.
RESULTS:
The attenuated tumour targeting Salmonella typhimurium SL7207 strain was modified to carry chromosomally integrated shRNA expression cassettes at the xylA locus. The colorectal cancer cell lines SW480, HCT116 and breast cancer cell line MCF7 were used to demonstrate the ability of these modified strains to perform intracellular infection and deliver effective RNA and protein knockdown of the target gene c-Myc. In vivo therapeutic efficacy was demonstrated using the Lgr5creERT2Apcflx/flx and BlgCreBrca2flx/flp53flx/flx orthotopic immunocompetent mouse models of colorectal and breast cancer, respectively. In vitro co-cultures of breast and colorectal cancer cell lines with modified SL7207 demonstrated a significant 50-95% (P < 0.01) reduction in RNA and protein expression with SL7207/c-Myc targeted strains. In vivo, following establishment of tumour tissue, a single intra-peritoneal administration of 1x106 CFU of SL7207/c-Myc was sufficient to permit tumour colonisation and significantly extend survival with no overt toxicity in control animals.
CONCLUSIONS:
In summary we have demonstrated that tumour tropic bacteria can be modified to safely deliver therapeutic levels of gene knockdown. This technology has the potential to specifically target primary and secondary solid tumours with personalised therapeutic payloads, providing new multi-cancer detection and treatment options with minimal off-target effects. Further understanding of the tropism mechanisms and impact on host immunity and microbiome is required to progress to clinical translation.
Safety and efficacy of N-acetylcysteine (NAC) as an adjunct to standard treatment in patients with acute ischemic stroke – A Randomized controlled trial (NACTLYS)
Authors: Komakula Snigdha , Bhatia Rohit , Sahib Akhil , Upadhyay Ashish , MBBS Leve Joseph , Garg Ajay , VY Vishnu , KishorPandit Awadh , Vibha Deepti , Singh Mamta Bhushan , Tripathi Manjari , Srivast MV Padma 
Published: September 2023

Abstract
Background There is a clinical need for thrombolytic agents that can disaggregate arterial thrombi without significantly increasing the risk of bleeding. The present study was aimed to study if adjunctive therapy with N-acetylcysteine (NAC) along with standard thrombolytic agent was safe and effective in patients with acute ischemic stroke as thrombolytic agent.
Methods An open label randomized blinded assessor pilot study was conducted. Patients with acute ischemic stroke presented to the emergency within 4.5 hours were randomized into two groups and intravenous NAC with intravenous tissue plasminogen activator (IV tPA) or IV tPA alone was given in a 1:1ratio. Primary outcomes were any intracerebral hemorrhage or symptomatic cerebral hemorrhage, any major or minor systemic bleeding and any adverse reactions. Secondary outcomes were major neurological improvement assessed by (National Institute of Health Stroke Scale) NIHSS at 24 hours, recanalization on first run of angiography in patients who underwent thrombectomy or on repeat vascular imaging at 24 hours, modified Rankin scale and mortality at 3 months of follow-up.
Results A total of 40 patients were enrolled out of which 21 patients received rtPA only and 19 patients received NAC with alteplase. There was no significant difference in baseline characteristics among both the groups. Among primary outcomes, no significant difference was noted in the adverse events(p=0.99), intracranial hemorrhage(p=0.21), symptomatic intracerebral hemorrhage (p=0.47) and extracranial bleeding (p=0.21). Among the secondary outcomes, median NIHSS at 24 hours was significantly low in the control arm (p=0.03). The proportion of patients with mRS 0-2 at 3 months and mortality at 3 months was comparable among both the groups (p=0.85 and p=0.99 respectively).
Conclusion N-acetylcysteine as an adjunct to alteplase has not shown significant difference in
morbidity, mortality at3months and safety profile. However, there was slightly better early neurological outcome with co- administration of N-acetylcysteine and alteplase. These indifference
might be due to the small size of the present study. Future larger trials are needed to further conclude on safety and efficacy of N-acetylcysteine as a thrombolytic agent.
Equine Embryo Production by Means of Double Ovulation Protocols in Colombian Creole Mares
Authors: Vargas José Luis Porras , Molano Carlos Eduardo Rodríguez , Romero Melisa Daniela Munevar , Torres Sergio Ulloa , Suarez Néstor Julián Pulido 
Published: September 2023

Abstract
Equine reproductive efficiency can be improved through the development of technologies such as embryo transfer, where the use of protocols that allow the presence of double ovulations can contribute to increasing the number of offspring per female per year. The objective was to evaluate the presence of double ovulation and embryos in Colombian Creole mares treated with deslorelin and histrelin. Twenty mares were taken and divided into 2 groups as follows: Treatment 1: 150 mg of deslorelin every 12 hours intramuscularly. Treatment 2: 80 mg of histrelin every 12 hours intramuscularly. In both treatments, the hormone was administered until two follicles with diameters greater than 35 mm were found to administer 2,000 U. I of human chorionic gonadotropin and were artificially inseminated 12 to 24 hours later with 300 million motile spermatozoa. Seven days after insemination, uterine lavage was performed to collect embryos. It was found that 60% of the females treated with histrelin presented double ovulation with a follicular diameter of 31.7 mm, and 8 mares had double embryos, while 45% of the mares treated with deslorelin presented double ovulation with a follicular diameter of 33 mm, and 5 mares had double embryos. An association between the presence of double ovulation and the presence of embryos was found in 45% of the mares treated with deslorelin. It is concluded that histrelin treatment presented better results in obtaining double ovulation in mares.
Caregiver Perspectives on Patients with Mucopolysaccharidosis II Treated with Pabinafusp Alfa: Results of Qualitative Interviews in Japan
Authors: Nakamura Kimitoshi , Sakai Norio , Hossain Mohammad Arif , Eisengart Julie B. , Yamamoto Tatsuyoshi , Tanizawa Kazunori , So Sairei , Schmidt Mathias , Sato Yuji 
Published: September 2023

Abstract
Background
Mucopolysaccharidosis type II (MPS II), or Hunter syndrome, is a rare inherited X-linked metabolic disorder predominantly affecting males. Pabinafusp alfa, an iduronate-2-sulfatase enzyme that can cross the blood-brain barrier, was approved in Japan in 2021 for use in the first enzyme replacement therapy targeting both the neuropathic and somatic symptoms of MPS II. This study explores the experiences of MPS II patients receiving pabinafusp alfa through qualitative interviews with their caregivers.
Methods
A semi-structured moderation guide (Voice of the Caregiver guide) was used to conduct semi-structured, qualitative interviews with caregivers at clinical sites in Japan. The interview transcripts underwent thematic analysis to identify symptoms and health-related quality of life impacts at baseline, changes since treatment began, and treatment experience.
Results
Seven caregivers were interviewed, representing seven children aged 8–18 years who had received pabinafusp alfa treatment for 3.3–3.5 years at the time of the interviews. The collective data suggest a general trend towards improvement, although not all caregivers observed discernible changes. Cognitive improvements encompassed language skills, concentration, self-control, eye contact, mental clarity, concept understanding, following instructions, and expressing personal needs. Changes included improvements in motor function and mobility, as well as musculoskeletal and somatic changes, such as organ involvement, joint mobility, sleep patterns, and fatigue. Four caregivers reported improvements in family quality of life, five reported treatment satisfaction, and all seven expressed a strong willingness to continue treating the children with pabinafusp alfa.
Conclusion
This study of caregivers’ perspectives on the qualitative experiences of patients treated with pabinafusp alfa showed treatment satisfaction and multiple quality of life improvements following therapy. These findings expand understanding of the benefit of using pabinafusp alfa to treat MPS II, and they should be helpful in defining MPS II-specific outcome measures to better determine treatment response in future clinical trials.
First Record of a Rare Species Atlantic Triple Tail, Lobotes Surinamensis (Bloch, 1790) From the Northwest Coast of India
Authors: Parmar Jeet , Borichangar Ritesh , Kardani Hitesh , Vyas Upasana 
Published: September 2023

Abstract
During our taxonomical research on rare fish species along the Northwest Coast of India, we documented the presence of Lobotes surinamensis, (Atlantic triple tail) species at two different locations along the Gujarat coast. One specimen was caught by Wada fishing in the Gulf of Kutch, the northwest coast, and another from Gholvu net from the southwest coast of Gujarat. While commercial fisheries have received significant focus, there has been a lack of studies conducted in the artisanal fisheries sector. In this study, we applied the classical taxonomical method for species identification. For the identification of specimens, we recorded morphometric parameters and meristic characters. The comparison was done with past records, upon close examination of all characteristics. Its presence has been reported from various parts of the globe and the east coast of India. This study confirms the first report of L. surinamensis on the northwest coast of India.
Implementation of Direct Battery Driven Discharge in Triggerless Operation of Vacuum Arc Thrusters
Authors: Saletes James , Saddul Kash , Wittig Alexander , Kim Minkwan 
Published: September 2023

Abstract
The increasing demand for CubeSat missions with limited budgets and constrained timelines necessitates the development of innovative propulsion systems. This paper focuses on vacuum arc thrusters (VATs) and investigates the implementation of battery-driven discharge for triggerless firing. VATs offer a promising alternative for CubeSat propulsion, generating thrust through the pulsed ejection of plasma in a cathode arc discharge. The study explores the feasibility of direct battery-driven discharge in VATs and demonstrates stable and variable-duration pulses. Experimental testing using high C-rating lithium-ion (Li-ion) batteries showcase the advantages and operational benefits of this approach. The results highlight the potential for variable energy pulses, precise control of thrust impulse bit, and improved performance compared to capacitor-driven systems. Future research directions include optimising pulse duration for enhanced lifetime, plume analysis, and erosion rate investigations. The implementation of VATs with battery-driven discharge presents a promising solution to enhance the performance and reliability of CubeSat propulsion systems while ensuring compliance with space sustainability guidelines.
Social Climate of Physical Training Classes of The Chinese Universities and the Undergraduate Students’ Mental Health, Physical Activities and Life Style
Authors: Wei Xiaowei , Yang Jian , Wei Chunyan , He Qiling 
Published: September 2023

Abstract
Background
The social climate within educational settings has been recognized as a significant factor influencing students' psychological well-being, behaviors, and overall quality of life. In the context of Chinese universities, where physical training classes play a crucial role in the holistic development of undergraduate students, understanding the relationship between the social climate of these classes and students' mental health engagement in physical activities, and lifestyle choices becomes paramount.
Objectives
This study aims to investigate the correlation between the social climate of physical training classes/ environment in Chinese universities and various aspects of undergraduate students' physical activities, healthy lifestyle, and mental health.
Method
384 undergraduate students from …. University in China were selected through connivence sampling. The participants attempted mental health, healthy lifestyle, caring climate and physical activities scales. Pearson correlation was used for analyzing the data (p < 0.05).
Results
Results show that there is a significant negative correlation between the students’ perceived social climate and their mental health. However, the correlation between the social climate and the students’ physical activities, healthy lifestyle, and dimensions of lifestyle were significantly positive.
Conclusion
In conclusion, this study sheds light on the intricate relationship between the social climate of physical training classes within Chinese universities and the multifaceted dimensions of undergraduate students' well-being.
Implications:
The implications of these findings are noteworthy for educators, policymakers, and practitioners involved in the design and management of educational settings. The negative correlation observed between students' perceived social climate and mental health underscores the need for concerted efforts to create supportive and nurturing environments within physical training classes. Strategies aimed at fostering a sense of belonging, emotional safety, and positive social interactions could contribute to enhanced mental well-being among students.
Molecular characterization and function of hif1a and fih1 in response to acute thermal stress in American shad (Alosa sapidissima)
Authors: Liang Zhengyuan , Hu Songqin , Dong Yalun , Miao Linghong , Zhu Wenbin , Feng Bingbing , Fu Jianjun , Luo Mingkun , Wang Lanmei , Dong Zaijie 
Published: September 2023

Abstract

In order to evaluate the function of hypoxia-inducible factor-1 alpha (hif1α) and factor inhibiting hif1α (fih1) in response to thermal stress, we firstly conducted functional analysis of A. sapidissima hif1α and fih1, and determined hif1α and fih1 expressions in different tissues in response to thermal stress based on identified housekeeping genes (HKGs). The results showed hif1α and fih1 were mainly located in the nucleus and cytoplasm. The full length cDNA sequence of hif1α and fih1 was 4073 bp and 2759 bp, respectively. The cDNA sequence of hif1α includes 15 exons encoding 750 amino acid residues and the full length cDNA sequence of fih1 contains 9 exons encoding 354 amino acid residues. During the acute thermal stress transferring from 16±0.5 oC (control) to 20±0.5 oC, 25±0.5 oC, and 30±0.5 oC for 15 min, it was found that the expression trends of hif1α and fih1 showed an inhibitory regulation in the heart, while they consistently expressed in other tissues. In conclusion, this is the first study to identify the tissue-specific HKGs in A. sapidissima and found that ef1α and β-actin are the most suitable HKGs. Hif1α and Fih1 is mainly the nuclear protein and cytoplasmic protein, respectively, both having high level in the heart and brain. Alosa sapidissima countered a temperature increasing from 16 ℃ to 25 ℃ by regulating the expressions of hif1α and fih1, but its physiological regulatory function was unable to cope with acute thermal stress at a temperature difference of 14 ℃ (from 16 ℃ to 30 ℃).
Effects of NaCl application on Cs concentration, number, and size of epidermal bladder cells in quinoa plants
Authors: Isobe Katsunori , Wada Kengo Shota Oishi・Masao , Oishi Shota , Higo Masao 
Published: September 2023

Abstract
Quinoa (Chenopodium quinoa Willd.) plants possess epidermal bladder cells (EBCs) on the leaf surface that accumulate excess Na+. However, whether excess Cs+ is transported from the leaf to the EBCs has not been elucidated in quinoa plants. In this study, the Cs+ concentration of EBCs and leaves of quinoa plants grown in soil treated with high concentrations of NaCl was investigated via pot experiments. Three different treatments were performed: 9.75 g plot, and 19.50 g plot, and a control (with no added NaCl). In 9.75 g plot and 19.50 g plot, 9.75 g and 19.5 g of NaCl were applied to the soil, respectively. And 0.10 g of CsCl were applied to all pots. We observed that Na+ concentration in EBCs and in leaves with and without EBCs increased with increasing NaCl concentration at the vegetative and flowering stages; Na+ concentration was lower in the EBCs than in both types of leaves at both growth stages. Cs+ concentration in EBCs and both types of leaves increased with increasing NaCl concentration; Cs+ concentration was higher in EBCs than in both types of leaves. However, NaCl application did not affect the number of EBCs at both growth stages, but the number of EBCs in older leaves was lower than that in younger leaves at both growth stages. Moreover, EBC diameter increased with NaCl application at both growth stages; the EBCs of younger leaves were larger than those of older leaves at both growth stages. Therefore, NaCl increased the Cs+-accumulation capacity of quinoa plants by increasing the size of the EBCs.