bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enrichment of miR-17-5p enhances the protective effects of EPC-EXs on vascular and skeletal muscle injury in a diabetic hind limb ischemia model
Authors: Pan Qunwen , Xu Xiaobing , He Wen , Wang Yan , Xiang Zhi , Jin Xiaojuan , Tang Qiong , Zhao Ting , Ma Xiaotang 
Published: April 2023

Abstract
Background/aims
Diabetes mellitus (DM) is highly susceptible to diabetic hind limb ischemia (DHI). MicroRNA (MiR)-17-5p is downregulated in DM and plays a key role in vascular protection. Endothelial progenitor cell (EPC)-released exosomes (EPC-EXs) contribute to vascular protection and ischemic tissue repair by transferring their contained miRs to target cells. Here, we investigated whether miR-17-5p-enriched EPC-EXs (EPC-EXsmiR-17-5p) had conspicuous effects on protecting vascular and skeletal muscle in DHI in vitro and in vivo.

Methods
EPCs transfected with scrambled control or miR-17-5p mimics were used to generate EPC-EXs and EPC-EXsmiR-17-5p. Db/db mice were subjected to hind limb ischemia. After the surgery, EPC-EXs and EPC-EXsmiR-17-5p were injected into the gastrocnemius muscle of the hind limb once every 7 days for 3 weeks. Blood flow, microvessel density, capillary angiogenesis, gastrocnemius muscle weight, structure integrity, and apoptosis in the hind limb were assessed. Vascular endothelial cells (ECs) and myoblast cells (C2C12 cells) were subjected to hypoxia plus high glucose (HG) and cocultured with EPC-EXs and EPC-EXsmiR-17-5p. A bioinformatics assay was used to analyze the potential target gene of miR-17-5p, the levels of SPRED1, PI3K, phosphorylated Akt, cleaved caspase-9 and cleaved caspase-3 were measured, and a PI3K inhibitor (LY294002) was used for pathway analysis.

Results
In the DHI mouse model, miR-17-5p was markedly decreased in hind limb vessels and muscle tissues, and infusion of EPC-EXsmiR-17-5p was more effective than EPC-EXs in increasing miR-17-5p levels, blood flow, microvessel density, and capillary angiogenesis, as well as in promoting muscle weight, force production and structural integrity while reducing apoptosis in gastrocnemius muscle. In Hypoxia plus HG-injured ECs and C2C12 cells, we found that EPC-EXsmiR-17-5p could deliver their carried miR-17-5p into target ECs and C2C12 cells and subsequently downregulate the target protein SPRED1 while increasing the levels of PI3K and phosphorylated Akt. EPC-EXsmiR-17-5p were more effective than EPC-EXs in decreasing apoptosis and necrosis while increasing viability, migration, and tube formation in Hypoxia plus HG-injured ECs and in decreasing apoptosis while increasing viability and myotube formation in C2C12 cells. These effects of EPC-EXsmiR-17-5p could be abolished by a PI3K inhibitor (LY294002).

Conclusion
Our results suggest that miR-17-5p promotes the beneficial effects of EPC-EXs on DHI by protecting vascular ECs and muscle cell functions.
Antiurolithiatic effect of a polyherbal formulation against sodium oxalate-induced urolithiasis in rats
Authors: Sayed Amany A. , Soliman Amel M. , Fahmy Sohair R. , Hosny Rasha 
Published: April 2023

Abstract
Background
The present study assesses the role of polyherbal formula (LACTN) against sodium oxalate (NaOx)—stimulated urolithiasis prophylactic and curative. Forty-eight rats were separated into the following regimen’s groups: Groups I, II, III, and IV (prophylactic groups). Group I (control group) received saline as vehicle. Group II (urolithiatic group) received 70 mg NaOx / kg b.wt, i.p. Groups III and IV received LACTN and cystone prophylactic (500 and 750 mg/kg b.wt, respectively) from 1st day to 7th day of urolithiasis induction. Groups V, VI, VII, and VIII (curative groups): these were divided into the prophylactic regimes, but LACTN and cystone were administered from 7th day to 14th day of urolithiasis induction.

Results
Urolithiatic rats co-/post-treated with LACTN and cystone elevated the urinary volume significantly (P < 0.05). Also, a significant decrease (P < 0.05) was observed in the serum and urine calcium and phosphate concentrations beside the increased magnesium value in urolithiatic rats co-/post-treated with LACTN. LACTN significantly (P < 0.05) ameliorates serum and urine creatinine, urea, and uric acid concentration. Moreover, urolithiatic rats co-/post-treated with LACTN significantly (P < 0.05) diminished MDA and increased GSH, GST, SOD, and CAT compared with urolithiatic rats.

Conclusions
LACTN has anti-lithogenesis effect that may be due to its ability to remove the already presenting stone and/or to prevent the generation of extra calcium oxalate crystals. This action may be due to its components’ synergetic action that may provide encouraging combined formula to prevent/treat urolithiasis.
Association between MTHFR C677T variant and risk for congenital heart defects in Egyptian children: a case–control study including meta-analysis based on 147 cases and 143 controls
Authors: Esmaiel Nora N. , Ashaat Engy A. , Al-Ettribi Ghada M. , Fayez Alaaeldin , Alsaiedi Sonia A. , El Ruby Mona O. 
Published: April 2023

AbstractBackgroundStratification analysis studies showed that ethnicity has a significant association regardingMTHFRC677T variant and congenital heart diseases (CHDs) risk, and many published studies have controversial conclusions toward this association.MethodsIn this study, the association between theMTHFRC677T variant and the risk for CHDs was evaluated in 91 children with CHD and 95 healthy controls, as new cases, by using restriction fragment length polymorphism (RFLP) technique. Besides that, 2 case–control studies in the Egyptian population published before 2021 were included in this meta-analysis. The association was assessed by the odds ratio (OR) with a 95% confidence interval (CI) based on 294 alleles in CHD cases and 286 alleles in controls.ResultsThe overall meta-analysis showed a significant association betweenMTHFRC677T variant and CHDs risk in Egyptian children with heterogeneity (Heterogeneity = 0.001) in all the genetic models with the highly significant association in T versus C allele (pooled OR 1.89, 95% CI 1.31–2.74;pvalue < 0.0004). The consistency of the genotypes was detected by Hardy–Weinberg equilibrium (HWE).ConclusionsOur results support theMTHFR-677T allele as a susceptibility factor for CHDs in the Egyptian pediatric patients.
Interaction of peripheral CD4+CD25+CD127− Tregs with prolactin in HCV hepatocellular carcinoma: oncogenic or immunogenic mechanisms
Authors: El-Makarem Mona Abd El-Rahman Abu , Sayed Douaa Mohammed , Matta Ragaa Abdelshaheed , Mohamed Mahmoud Ragab , El-Malak Manal Ameer Abd , Abbas Nehal Ibrahim 
Published: April 2023

Abstract
Background and objective
There is little and conflicting data about the peripheral CD4+CD25+CD127− Tregs in patients with hepatocellular carcinoma (HCC) of various etiologies. The expressed membrane-bound transforming growth factor (mTGF-β1) on these Tregs is a marker of their suppressive function. Prolactin suppresses Tregs function in healthy subjects but enhances local Tregs in breast cancer. Our study is the first to assess the frequency and function of CD4+CD25+CD127−Tregs and their association with clinicopathological features and staging in HCV-related HCC and to determine whether prolactin acts as an oncogenic growth factor or participates in the regulation of the immune response mediated by peripheral Tregs. In patients with HCV- elated HCC, HCV-cirrhotic patients, and healthy subjects, we measured the frequency of peripheral traditional CD4+ CD25+ Tregs and well-characterized CD4+CD25+CD127−Tregs and their mTGF-β1 using flow cytometric analysis and measured serum prolactin level.

Results
The frequency of CD4+ CD25+ and CD4+CD25+CD127− Tregs was comparable between HCC and cirrhotic patients and healthy subjects. Serum prolactin and mTGF-β1 on traditional and CD4+CD25+CD127− Tregs were significantly higher in HCC and cirrhotic patients than healthy subjects with an insignificant difference between HCC and cirrhotic patients. Roc curve analysis revealed that cutoff value for mTGF-β1 on Tregs ≥ 13.5% is a good specific (87%) but low sensitive (54%) test in discriminating HCC patients from healthy subjects. The frequency of Tregs and mTGF-β1 were not correlated to clinicopathological characteristics or staging of HCC. Prolactin was higher in the multifocal lesions and negatively correlated to expressed mTGFβ1. The expressed mTGF-β1 was positively correlated with hemoglobin and alanine transaminase. The traditional Tregs was positively correlated with hemoglobin and albumin.

Conclusion
mTGFβ1, as a marker for suppressive function of peripheral CD4 + CD25 + CD127-Tregs, has a diagnostic role in discriminating HCV-related HCC patient from healthy subjects, unfortunately not from HCV-related cirrhotic patients. Serum prolactin has an oncogenic role as it is correlated to multiple focal lesions. It also impedes the suppressive function of peripheral Tregs as an immunogenic role. mTGF-β1 is related to hemoglobin and hepatic inflammation.
Associations of activity, sedentary, and sleep behaviors with cognitive and social-emotional health in early childhood
Authors: St. Laurent Christine W. , Rasmussen Charlotte Lund , Holmes Jennifer F. , Cremone-Caira Amanda , Kurdziel Laura B. F. , Desrochers Phillip C. , Spencer Rebecca M. C. 
Published: April 2023

Abstract
Background
Early childhood is important for cognitive and social-emotional development, and a time in which to promote healthy movement behaviors (sedentary behavior, physical activity, and sleep). Movement behaviors may have interactive influences on cognition and social-emotional factors in young children, but most previous research has explored them independently. The purpose of this study was to determine if movement behaviors are associated with measures of cognitive and social-emotional health in young children and if so, to describe optimal compositions of movement behaviors of a daily cycle for such outcomes.

Methods
Children (n = 388, 33 to 70 months, 44.6% female) from a clinical trial (ClinicalTrials.gov ID: NCT03285880, first posted September 18, 2017) wore accelerometers on their wrists for 24-h for 9.56 ± 3.3 days. Movement behavior compositions consisted of time spent in sedentary behaviors, light intensity physical activity, moderate to vigorous intensity physical activity (MVPA), and sleep. Outcomes were cognitive (receptive vocabulary, declarative and procedural memory, and executive attention) and social-emotional measures (temperament and behavioral problems). Compositional linear regression models with isometric log ratios were used to investigate the relations between the movement behavior composition and the cognitive and social-emotional health measures. If a significant association was found between the composition and an outcome, we further explored the “optimal” 24-h time-use for said outcome.

Results
Movement behavior compositions were associated with receptive vocabulary. The composition associated with the predicted top five percent of vocabulary scores consisted of 12.1 h of sleep, 4.7 h of sedentary time, 5.6 h of light physical activity, and 1.7 h of MVPA.

Conclusions
While behavior compositions are related to vocabulary ability in early childhood, our findings align with the inconclusiveness of the current evidence regarding other developmental outcomes. Future research exploring activities within these four movement behaviors, that are meaningful to cognitive and social-emotional development, may be warranted.
Primary pulmonary pleomorphic liposarcoma found with a massive hemothorax
Authors: Ohsawa Fumi , Matsuura Natsumi , Numajiri Kazuki , Igai Hitoshi , Kamiyoshihara Mitsuhiro 
Published: April 2023

Abstract
Background
Pleomorphic liposarcoma (PLPS) is a rare and aggressive subtype of liposarcoma. Here we report the case of a 45-year-old man with PLPS in the lung, which was accompanied by a massive hemothorax.

Case presentation
The patient presented to hospital with chest pain. A chest x-ray showed massive right pleural effusion. We drained the right chest cavity of 1 L of blood and enhanced computed tomography (CT) revealed a massive right hemothorax and a 78 × 70 mm mass in the right lower lung lobe. The tumor was ruptured and bleeding, so we performed right lower lobe resection. Pathological investigations revealed a PLPS. One month after surgery, positron emission tomography-CT showed considerable pleural dissemination in the right lung field. The patient died 3 months after surgery.

Conclusions
PLPS of the lung is rare, but requires attention because it can cause tumor rupture and hemothorax due to rapid tumor growth, and which may make complete resection of tumor difficult or necessitate emergency surgery.
Determination of the Optimal Volume of Programmed Intermittent Epidural Bolus When Combined With the Dural Puncture Epidural Technique for Labor Analgesia: A Random-Allocation Graded Dose-Response Study
Authors: Xiao Fei , Yao Han-Qing , Qian Jing , Deng Jia-Li , Xu Zheng-Fen , Liu Lin , Chen Xin-Zhong 
Published: April 2023

BACKGROUND:
The dural puncture epidural (DPE) and the programmed intermittent epidural bolus (PIEB) techniques are recent innovations for labor analgesia. The optimal volume of PIEB during traditional epidural analgesia has been investigated previously but it is unknown whether these findings are applicable to DPE. This study aimed to determine the optimal volume of PIEB for effective labor analgesia after initiation of analgesia using DPE.

METHODS:
Parturients requesting labor analgesia received dural puncture with a 25-gauge Whitacre spinal needle and then had analgesia initiated with 15 mL of ropivacaine 0.1% with sufentanil 0.5 μg/mL. Analgesia was maintained using the same solution delivered by PIEB with boluses given at a fixed interval of 40 minutes starting 1 hour after the completion of the initial epidural dose. Parturients were randomized to 1 of 4 PIEB volume groups: 6, 8, 10, or 12 mL. Effective analgesia was defined as no requirement for a patient-controlled or manual epidural bolus for 6 hours after the completion of the initial epidural dose or until full cervical dilation. The PIEB volumes for effective analgesia in 50% of parturients (EV50) and 90% of parturients (EV90) were determined using probit regression.

RESULTS:
The proportions of parturients with effective labor analgesia were 32%, 64%, 76%, and 96% in the 6-, 8-, 10-, and 12-mL groups, respectively. The estimated values for EV50 and EV90 were 7.1 (95% confidence interval [CI], 5.9−7.9) mL and 11.3 (95% CI, 9.9−15.2) mL, respectively. There were no differences in side effects, including hypotension, nausea and vomiting, and fetal heart rate (FHR) abnormalities among groups.

CONCLUSION:
Under the conditions of the study, after initiation of analgesia using DPE, the EV90 of PIEB for effective labor analgesia using ropivacaine 0.1% with sufentanil 0.5 μg/mL was approximately 11.3 mL.
Women Representation in Anesthesiology Society Leadership Positions
Authors: Harbell Monica W. , McMullen Kaley B. , Kosiorek Heidi , Kraus Molly B. 
Published: April 2023

BACKGROUND:
Although the number of women in medicine has increased, women remain underrepresented in leadership positions, specifically in medical societies. Specialty societies in medicine are influential in networking, career advancement, research and education opportunities, and providing awards and recognition. The goals of this study are to examine the representation of women in leadership positions in anesthesiology societies compared to women society members and women anesthesiologists and to analyze the trend in women society presidents over time.

METHODS:
A list of anesthesiology societies was obtained from the American Society of Anesthesiology (ASA) website. Society leadership positions were obtained via the societies’ websites. Gender was determined by images on the society website and images or pronouns on hospital websites and research databases. The percentage of women presidents, vice presidents/presidents-elect, secretaries/treasurers, board of directors/council members, and committee chairs was calculated. The percentage of women in society leadership positions was compared to the percentage of women society members when available, and the percentage of women anesthesiologists in the workforce (26%) using binomial difference of unpaired proportions tests. The trend of women presidents from 1980 to 2020 was analyzed using a Cochran-Armitage trend test.

RESULTS:
A total of 13 societies were included in this study. Overall, women held 32.6% (189/580) of leadership positions. 38.5% (5/13) of presidents, 17.6% (3/17) of presidents-elect/vice presidents, and 45% (9/20) of secretaries/treasurers were women. In addition, 30.0% (91/303) of board of directors/council members and 34.2% (90/263) of committee chairs were women. The percentage of women holding society leadership positions was significantly greater than the percentage of women anesthesiologists in the workforce (P < .001), as was the percentage of women as committee chairs (P = .003). The percentage of women society members was available for 9 of 13 societies (69%), and the percentage of women leaders was similar to the percentage of women society members (P = .10). There was a significant difference in the percentage of women leaders between society size categories. Small societies had 32.9% (49/149) women leaders, medium had 39.4% (74/188) women leaders, and the single large society had 27.2% (66/243) (P = .03). There were also significantly more women leaders in the Society of Cardiovascular Anesthesiologists (SCA) than there are women members (P = .02).

CONCLUSIONS:
This study suggests that anesthesia societies may be more inclusive of women in leadership positions compared to other specialty societies. Although in anesthesiology, women remain underrepresented in academic leadership roles, there is a higher proportion of women in leadership roles in anesthesiology societies than proportion of women in the anesthesia workforce.
Another Arabia
Authors: Bsheer Rosie 
Published: April 2023

abstractThis article takes up the occluded history of a particular category of migrant—the migrant scholar—in late Ottoman Mecca. It does so through the trajectory of the prominent Indian religious and anti-colonial scholar Muhammad Rahmatullah al-Kairanawi (1818–1891) and the afterlives of al-Sawlatiyya, the school he founded in 1873 in Mecca, where many South Asian and other scholars and rebels sought refuge in the nineteenth and early twentieth centuries. Through his teaching and public activism in Mecca, he built the scaffolding of a long intellectual and political legacy. Kairanawi and other scholar-activists brought with them a panoply of anti-colonial and modernist ideas—secular and religious, reformist and revolutionary. Exposing the centrality of migrant scholars to the social, intellectual, and political fabrics of Arabian and South Asian lifeworlds reveals another Arabia, one that demolition and historical revision—now mundane universal practices—seek to permanently erase. Doing so also delivers profound lessons on the figure of the migrant as scholar, on the imperative of transcending national history, and on thinking of history itself as punctured by continuous crises.
A Systematic Review Comparing Focused Ultrasound Surgery With Radiosurgery for Essential Tremor
Authors: Kondapavulur Sravani , Silva Alexander B. , Molinaro Annette M. , Wang Doris D. 
Published: April 2023

BACKGROUND:
Focused ultrasound (FUS-T) and stereotactic radiosurgery thalamotomy (SRS-T) targeting the ventral intermediate nucleus are effective incisionless surgeries for essential tremor (ET). However, their efficacy for tremor reduction and, importantly, adverse event incidence have not been directly compared.

OBJECTIVE:
To present a comprehensive systematic review with network meta-analysis examining both efficacy and adverse events (AEs) of FUS-T vs SRS-T for treating medically refractory ET.

METHODS:
We conducted a systematic review and network meta-analysis according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, using the PubMed and Embase databases. We included all primary FUS-T/SRS-T studies with approximately 1-year follow-up, with unilateral Fahn-Tolosa-Marin Tremor Rating Scale or Clinical Rating Scale for Tremor scores prethalamotomy/post-thalamotomy and/or AEs. The primary efficacy outcome was Fahn-Tolosa-Marin Tremor Rating Scale A+B score reduction. AEs were reported as an estimated incidence.

RESULTS:
Fifteen studies of 464 patients and 3 studies of 62 patients met inclusion criteria for FUS-T/SRS-T efficacy comparison, respectively. Network meta-analysis demonstrated similar tremor reduction between modalities (absolute tremor reduction: FUS-T: −11.6 (95% CI: −13.3, −9.9); SRS-T: −10.3 (95% CI: −14.2, −6.0). FUS-T had a greater 1-year adverse event rate, particularly imbalance and gait disturbances (10.5%) and sensory disturbances (8.3%). Contralateral hemiparesis (2.7%) often accompanied by speech impairment (2.4%) were most common after SRS-T. There was no correlation between efficacy and lesion volume.

CONCLUSION:
Our systematic review found similar efficacy between FUS-T and SRS-T for ET, with trend toward higher efficacy yet greater adverse event incidence with FUS-T. Smaller lesion volumes could mitigate FUS-T off-target effects for greater safety.
Mortality due to traffic accidents in Colombia: Profiles of pedestrians and cyclists, 1998-2019
Authors: Montenegro-Martínez Gino , Agudelo-Cifuentes Maite-Catalina , Muñoz-Rodriguez Diana-Isabel 
Published: April 2023

Background: Traffic accidents are an important issue for public health and a threat for sustainable development, with pedestrians and cyclists having been recognized as the most vulnerable actors on the streets. The objective of this study was to analyze the profiles of pedestrians and cyclists who died as a result of traffic accidents in Colombia during the 1998-2019 period. Methods: An observational and descriptive study, with the deaths due to traffic accidents in Colombia between 1998 and 2019 as data source. Secondary data were taken from the Vital statistics of Colombia (EEVV), published by Departamento Administrativo Nacional de Estadística (DANE). A trend analysis of the number of deaths during the period under study was performed, and such number was examined against sex to identify potential differences. Multiple correspondence analysis was employed to elaborate the profile of pedestrians and cyclists who die due to traffic accidents. Three profiles were prepared for each road actor: a global profile, one for 1998, and another for 2019. Results: The mortality profiles are different for pedestrians and cyclists, and, in turn, there are also demographic, geographic, and socioeconomic conditions in each type of road actor, which determine higher mortality risks. High population density, younger age group in the cyclists and adults among the pedestrians, low schooling levels and absence of health insurance are suggested as key factors in these profiles. Related to sex, for men is not possible to establish a profile. Women's cases are commonly related to health insurance, age, and population density. Conclusions: Several contextual and demographic characteristics in pedestrians and cyclists allow delimiting mortality profiles. The profiles that were identified suggest the need to articulate road safety policies with other social and development policies in order to coordinate and integrate intersectoral actions that reduce mortality in these road actors.