bluuebunny/crossref_metadata_2025_split huggingface dataset:
Highly sensitive liquid biopsy Duplex sequencing complements tissue biopsy to enhance detection of clinically relevant genetic variants
Authors: Hallermayr Ariane , Neuhann Teresa M. , Steinke-Lange Verena , Scharf Florentine , Laner Andreas , Ewald Roland , Liesfeld Ben , Holinski-Feder Elke , Pickl Julia M. A. 
Published: December 2022

BackgroundLiquid biopsy (LB) is a promising complement to tissue biopsy for detection of clinically relevant genetic variants in cancer and mosaic diseases. A combined workflow to enable parallel tissue and LB analysis is required to maximize diagnostic yield for patients.MethodsWe developed and validated a cost-efficient combined next-generation sequencing (NGS) workflow for both tissue and LB samples, and applied Duplex sequencing technology for highly accurate detection of low frequency variants in plasma. Clinically relevant cutoffs for variant reporting and quantification were established.ResultsWe investigated assay performance characteristics for very low amounts of clinically relevant variants. In plasma, the assay achieved 100% sensitivity and 92.3% positive predictive value (PPV) for single nucleotide variants (SNVs) and 91.7% sensitivity and 100% PPV for insertions and deletions (InDel) in clinically relevant hotspots with 0.5-5% variant allele frequencies (VAFs). We further established a cutoff for reporting variants (i.e. Limit of Blank, LOB) at 0.25% VAF and a cutoff for quantification (i.e. Limit of Quantification, LOQ) at 5% VAF in plasma for accurate clinical interpretation of analysis results. With our LB approach, we were able to identify the molecular cause of a clinically confirmed asymmetric overgrowth syndrome in a 10-year old child that would have remained undetected with tissue analysis as well as other molecular diagnostic approaches.ConclusionOur flexible and cost-efficient workflow allows analysis of both tissue and LB samples and provides clinically relevant cutoffs for variant reporting and precise quantification. Complementing tissue analysis by LB is likely to increase diagnostic yield for patients with molecular diseases.
Effect of Low-Temperature Tolerant Rootstocks on the Growth and Fruit Quality of Watermelon in Semi-Forcing and Retarding Culture
Authors: Jang Yoonah , Moon Ji-Hye , Kim Sang-Gyu , Kim Taebok , Lee Oak-Jin , Lee Hee-Ju , Wi Seung-Hwan 
Published: December 2022

Watermelon grafting is practiced in order to improve tolerance to poor environments such as low temperature. This study was conducted to investigate the effect of low-temperature tolerant bottle gourd (Lagenaria siceraria) rootstocks on the growth and fruit quality of watermelon in semi-forcing and retarding culture where plants were exposed to low or high temperature. Five bottle gourd accessions (FR79, IT207112, BG702, BG703, and FRD22) with low temperature tolerance were evaluated as rootstock for the watermelon scion ‘Sambokkul’. Non-grafted watermelon and watermelon grafted onto commercial rootstock ‘Shintozwa’ (Cucurbita maxima D. × C. moschata D.) or ‘Bullojangsaeng’ (L. siceraria) were used as controls. Watermelons were cultivated in spring (April to June, semi-forcing culture) and autumn (August to October, retarding culture). The responses to low-temperature, growth, yield, and fruit quality differed depending on the rootstocks and growing season. In semi-forcing culture, the monthly averages of daily and minimum temperature in April were, respectively, 13.4 and 1.5 °C. Although the low temperature of the early growth stage suppressed the initial growth of watermelons, grafting mitigated the low-temperature stress. The fruit quality of non-grafted watermelons was greater, but the fruit mass was the lowest (4.8 kg). Grafting onto ‘Shintozwa’ increased the fruit weight (7.0 kg) but reduced the fruit quality. Grafting onto bottle gourd rootstocks had high affinity, good root growth, tolerance to low temperature, and little effect on fruit quality. In retarding culture, the temperature conditions in early and late growth were very high and low, respectively. The growth and fruit quality of grafted watermelons were not superior to those of non-grafted watermelons. Accordingly, these results suggest that watermelon grafting onto the bottle gourd rootstocks may increase the low-temperature tolerance, especially in the early growth stage, and the marketable yield without a reduction in fruit quality. The most promising accession for this purpose was found to be ‘FR79’.
Low Transmission of Chikungunya Virus by Aedes aegypti from Vientiane Capital, Lao PDR
Authors: Calvez Elodie , Miot Elliott F. , Keosenhom Sitsana , Vungkyly Vaekey , Viengphouthong Souksakhone , Bounmany Phaithong , Brey Paul T. , Marcombe Sébastien , Grandadam Marc 
Published: December 2022

In 2012–2013, chikungunya virus (CHIKV) was the cause of a major outbreak in the southern part of Lao People’s Democratic Republic (Lao PDR). Since then, only a few imported cases, with isolates belonging to different lineages, were recorded between 2014 and 2020 in Vientiane capital and few autochthonous cases of ECSA-IOL lineage were detected in the south of the country in 2020. The CHIKV epidemiological profile contrasts with the continuous and intensive circulation of dengue virus in the country, especially in Vientiane capital. The study’s aim was to investigate the ability of the local field-derived Aedes aegypti population from Vientiane capital to transmit the Asian and ECSA-IOL lineages of CHIKV. Our results revealed that, for both CHIKV lineages, infection rates were low and dissemination rates were high. The transmission rates and efficiencies evidenced a low vector competence for the CHIKV tested. Although this population of Ae. aegypti showed a relatively modest vector competence for these two CHIKV lineages, several other factors could influence arbovirus emergence such as the longevity and density of female mosquitoes. Due to the active circulation of CHIKV in Southeast Asia, investigations on these factors should be done to prevent the risk of CHIKV emergence and spread in Lao PDR and neighboring countries.
Phytochemical Investigation of Marker Compounds from Indigenous Korean Salix Species and Their Antimicrobial Effects
Authors: Jang Yoon Seo , Lee Da Eun , Hong Joo-Hyun , Kim Kyung Ah , Kim Bora , Cho Yeo Rang , Ra Moon-Jin , Jung Sang-Mi , Yu Jeong-Nam , An Seongpil , Kim Ki Hyun 
Published: December 2022

Salix species, including willow trees, are distributed in the temperate regions of Asian countries, including South Korea. Willow trees are used to treat pain and inflammatory diseases. Due to the medicinal properties of willow trees, pharmacological studies of other Salix spp. have gained attention; however, only a few studies have investigated the phytochemicals of these species. As part of our ongoing natural product research to identify bioactive phytochemicals and elucidate their chemical structures from natural resources, we investigated the marker compounds from indigenous Korean Salix species, namely, Salix triandra, S. chaenomeloides, S. gracilistyla, S. koriyanagi, S. koreensis, S. pseudolasiogyne, S. caprea, and S. rorida. The ethanolic extract of each Salix sp. was investigated using high-performance liquid chromatography combined with thin-layer chromatography and liquid chromatography–mass spectrometry-based analysis, and marker compounds of each Salix sp. were isolated. The chemical structures of the marker compounds (1–8), 3-(4-hydroxyphenyl)propyl β-D-glucopyranoside (1), 2-O-acetylsalicin (2), 1-O-p-coumaroyl glucoside (3), picein (4), isograndidentatin B (5), 2′-O-acetylsalicortin (6), dihydromyricetin (7), and salicin (8) were elucidated via nuclear magnetic resonance spectroscopy and high-resolution liquid chromatography–mass spectrometry using ultrahigh-performance liquid chromatography coupled with a G6545B Q-TOF MS system with a dual electrospray ionization source. The identified marker compounds 1–8 were examined for their antimicrobial effects against plant pathogenic fungi and bacteria. Dihydromyricetin (7) exhibited antibacterial activity against Staphylococcus aureus, inducing 32.4% inhibition at a final concentration of 125 μg/mL with an MIC50 value of 250 μg/mL. Overall, this study isolated the marker compounds of S. triandra, S. chaenomeloides, S. gracilistyla, S. koriyanagi, S. koreensis, S. pseudolasiogyne, S. caprea, and S. rorida and identified the anti-Staphylococcus aureus bacterial compound dihydromyricetin.
The Effect of Banana Rhizosphere Chemotaxis and Chemoattractants on Bacillus velezensis LG14-3 Root Colonization and Suppression of Banana Fusarium Wilt Disease
Authors: Yang Lihua , Zhou You , Guo Lijia , Yang Laying , Wang Jun , Liang Changcong , Huang Junsheng 
Published: December 2022

Fusarium oxysporum f. sp. cubense (Foc) causes banana Fusarium wilt disease, which is a destructive soil-borne disease. Many plants can recruit rhizosphere microorganisms using their root exudates, thereby shaping the rhizosphere microbiome to resist pathogen infection. Therefore, this study was conducted to explore the role of root exudates in the process of biocontrol strain colonization and resistance to pathogens. In this study, the banana root exudates used as chemoattractants were obtained by hydroponics. Bacillus velezensis strain LG14-3 was isolated from the infected area of the root system of banana and showed significant chemotaxis to banana root exudates and strong inhibition of Fusarium oxysporum f. sp. cubense. Further analysis found that LG14-3 showed chemotaxis toward the components of banana root exudates, such as citric acid, succinic acid, glycine, D-galactose and D-maltose, and glycine and citric acid, which resulted in more significant chemotaxis of LG14-3. Moreover, banana root exudates enhanced the swarming motility and biofilm formation of LG14-3. Pot experiments showed that glycine and citric acid enhanced the colonization ability of Bacillus velezensis LG14-3 in the banana rhizosphere and reduced the disease severity index of banana fusarium wilt. Glycine and citric acid enhanced the growth-promoting ability of LG14-3 under pathogen stress. Our results showed that the addition of chemotactic substances enhanced the biocontrol potential of Bacillus velezensis LG14-3 to prevent banana Fusarium wilt.
From Perfect Secrecy to Perfect Safety & Security: Cryptography-Based Analysis of Endogenous Security
Authors: Hu Xiaoyan , Jin Liang , Wu Jiangxing 
Published: December 2022

<p>In this paper, we propose a conjecture that endogenous security without any prior knowledge is similar to perfect secrecy without any prior knowledge. To prove the conjecture, we first establish a cryptography model of instinct function security to transform the security problem in the network domain into an encryption problem in the cryptographic domain. Then, we inherit and apply the established ideas and means of the Perfect Secrecy, and propose the concept, definition and corollaries of the perfect instinct function security (PIFS) corresponding to the Perfect Secrecy. Furthermore, we take the DHR system as a concrete implementation of PIFS and propose the DHR Perfect Security Theorem corresponding to Shannon’s Perfect Secrecy Theorem. Finally, we prove that the DHR satisfying the "One-Time Reconstruction" constraint is the sufficient and necessary condition to achieve perfect security. This means that the existence of PIFS is also proven.</p>
<p>The analysis shows that any reconfigurable system can be encrypted by its construct and that the PIFS converts the one-way transparent superiority of the attacker into a double-blind problem for both the attacker and the defender, which leads to that the attacker is impossible to obtain useful construction information from the attacks and unable to find a better way than blind trial-and-error or brute-force attacks. </p>
<p>Since the attackers are required to have the new powerful ability to crack the structure cryptogram, the threshold of cyber security is raised to at least the same level as cryptogram deciphering, thereafter the ubiquitous cyber threats are destined to be significantly reduced.</p>
A Novel Melspectrogram Snippet Representation Learning Framework For Severity Detection of Chronic Obstructive Pulmonary Diseases
Authors: Roy Arka , Satija Udit 
Published: December 2022

<p> Chronic obstructive pulmonary disease (COPD) is a major public health concern across the world. Since it is an incurable disease, early detection and accurate diagnosis are very crucial for preventing the progression of the disease. Lung sounds provide reliable and accurate prognoses for identifying respiratory diseases. Recently, Altan et al. recorded 12-channel real-time lung sound dataset, namely RespiratoryDatabase@TR, for five different severity levels of COPD at Antakya State Hospital Turkey, and proposed deep learning frameworks for two-class COPD classification and five-class classification using a deep belief network (DBN) classifier and extreme learning machine (ELM) classifier respectively. A classification accuracy of 95.84% and 94.31% were achieved for two-class and five-class respectively. In this paper, we have proposed a melspectrogram snippet representation learning framework for both two-class and five-class COPD classification. The proposed framework consists of the following stages: preprocessing, melspectrogram snippet representation generation from lung sound and fine tuning of a pretrained YAMNet. Experimental analysis on the RespiratoryDatabase@TR dataset demonstrates that the proposed framework achieves accuracies of 99.25% and 96.14% for binary and multi-class COPD severity classification respectively, which is superior to the only existing methods proposed by Altan et al. for severity analysis of COPD using lung sounds. </p>
Chimeric Antigen Receptor T-Cell Therapy for Solid Tumors: The Past and the Future
Authors: Srour Samer A. , Akin Serkan 
Published: December 2022

ABSTRACT
Chimeric antigen receptor (CAR) T-cell therapy is the new standard treatment for various indications in patients with advanced hematologic malignancies. Despite the several preclinical and early phase clinical trials, the overall clinical experience has been disappointing when applying this innovative therapy in solid tumors. The failure of CAR T-cell therapy and its limited antitumor activity in solid tumors have been attributed to several mechanisms, including tumor antigen heterogeneity, the hostile tumor microenvironment and poor trafficking of CAR T cells into tumor sites, and the unacceptable toxicities in some settings, among others. However, remarkable improvements have been made in understanding many of these failure mechanisms for which several emerging novel approaches are being applied to overcome these challenges. In this review, after a brief historic background for immunotherapy in solid tumors, we highlight the recent developments achieved in CAR T-cell designs, summarize completed clinical trials, and discuss current challenges facing CAR T-cell therapy and the suggested strategies to overcome these barriers.
The Theme of the Quest of Fatherhood in Charles Dickens's Novel Great Expectations
Authors: Asst. Prof. Dr. Ali Mohammed Segar  
Published: December 2022

This research depends on the critical analysis method for the main events and characters in the novel. It assumes that the novelist Charles Dickens, in Great Expectations, like in his other novels, keeps on his attack on some specific abuses and injustices in the social system in England during the 19th century. From the early beginning to the end of the novel, there is a parallel and simultaneous quest. The central character, Pip is an orphan boy who needs and unconsciously searches for a father, and the convict Ebel Magwitch who has lost his daughter, is looking for fatherhood. The fate of the orphan child is very strangely linked to the chased fugitive criminal. The paper consists of a general introduction about the novelist and his remarkable career followed by three sections: the first section briefly reviews some of the previous studies about the novel, the second explains the main characters and the plot of the novel, the third discusses the main hypothesis of the research and a conclusion which summarizes, briefly, the findings of the research.
Employees’ Views on the Use of Social Media Platforms at Work
Authors: Paul Uchhas 
Published: December 2022

Nowadays, no public or private organization in Bangladesh can function without the widespread adoption of social networking platforms. It has recently replaced in-person social interactions and communication. Most modern organizations provide their employees with affordable Internet connections by PC, laptop, and some other mobile devices, allowing them quick access to personal and professional social networking sites from both home and work. There are many advantages to using social networking sites at work, but there are also some negatives. An organizational policy on the use of social networking sites while on the job is a powerful management tool. This research aims to learn more about how employees are utilizing social media at work as well as the effects of this behaviour. The research hopes to help organizations implement policies that are mutually agreeable to employees and management. A total of 60 people from a variety of occupations participated in the survey. Some of the primary ideas given in this paper that can work for the organization without restricting the freedom of the employees are: providing proper direction; teaching and training employees about the security risks for the organization; enabling employees with time-based access; etc.

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KINERJA DITINJAU DARI MOTIVASI INTRINSIK PADA TENAGA KEPENDIDIKAN
Authors: Dian Wishnu Brata  
Published: December 2022

Penelitian ini bertujuan untuk membuktikan hipotesis yang diajukan, yaitu: ada hubungan antara motivasi intrinsik dengan kinerja. Penelitian ini merupakan penelitian korelasional. Subjek penelitian sebanyak 62 Tenaga Kependidikan Fakultas Ilmu Pendidikan Universitas Negeri Semarang serta metode analisis data menggunakan teknik korelasi sederhana. Hasil penelitian menunjukkan nilai korelasi antar kedua variabel tersebut, yaitu 0,425 (P < 0,01) sehingga dapat disimpukan motivasi intrinsik dengan kinerja berkorelasi secara signifikan.
This study aims to prove the proposed hypothesis, namely: there is a relationship between intrinsic motivation and performance. This research is a correlational study. The research subjects were 62 Education Personnel of the Faculty of Education, State University of Semarang and the data analysis method used a simple correlation technique. The results showed that the correlation value between the two variables was 0.425 (P < 0.01) so that it could be concluded that intrinsic motivation and performance correlated significantly.
PENGARUH GAYA KELEKATAN TERHADAP TOXIC RELATIONSHIP PADA MAHASISWA TEKNIK ELEKTRO UNIVERSITAS SINGAPERBANGSA KARAWANG
Authors: Venia Nabila  , Wina Lova Riza  , Puspa Rahayu Utami Rahman  
Published: December 2022

Wawancara dilakukan kepada 21 mahasiswa Teknik Elektro Angkatan tahun 2019 Universitas Singaperbangsa Karawang. Hasil wawancara ditemukan bahwa mereka menjalani toxic relationship. Oleh karenanya, tujuan dari penelitian ini adalah untuk meneliti ada tidaknya pengaruh gaya kelekatan terhadap toxic relationship pada mahasiswa Teknik Elektro Universitas Singaperbangsa Karawang. Penelitian ini berbentuk kausal memakai metode penelitian kuantitatif dengan skala toxic relationship (Mayorita, 2021) dan skala ECR (Experience in Close Relationship) (Brennan, Clark & Shaver dalam Levine & Heller, 2019). Sampel dalam penelitian ini sebanyak 118 orang dari semua responden yang didapatkan. Data penelitian kemudian dianalisis dengan regresi linier sederhana dengan hasil sig 0,034 < 0,05 (H଴ ditolak dan Hୟditerima). Sehingga dapat disimpulan bahwa ada pengaruh gaya kelekatan terhadap toxic relationship pada mahasiswa Teknik Elektro Universitas Singaperbangsa Karawang yang artinya apabila seseorang yang memiliki gaya kelekatan tidak aman, maka akan mengalami gangguan pada relasi dengan orang lain maupun pasangan.
Interviews were conducted with 21 students of electrical engineering class of 2019 at the Singaperbangsa Karawang University. The results of the interview found that they were in a toxic relationship. Therefore, the purpose of this study is to examine if there is an influence of attachment style on toxic relationships in Electrical Engineering students at Singaperbangsa Karawang University. This research is causal form using quantitative research methods with a toxic relationship scale (Mayorita, 2021) and an attachment style scale ECR (Experience in Close Relationship) (Brennan, Clark & Shaver in Levine & Heller, 2019). The sample in this study was 118 people from all respondents who were obtained. The data in this study was analyzed by simple linear regression. Results showed sig 0,034 < 0,05 (H଴ is rejected and Hୟis accepted). So it can be concluded that there is an influence of attactment style on toxic relationships in Electrical Engineering students at Singaperbangsa Karawang University, which means that if someone has an insecure attachment style, they will experience disturbances in relationship with other people or partners.
CAUSES OF INDUSTRIAL INJURIES IN AIC
Authors: Лариса Николаевна Бердникова, 
Published: December 2022

В статье показана целесообразность предупреждения производственного травматизма, которое основывается на изучении и анализе причин аварий и несчастных случаев, участии в их расследовании и контроле за выполнением работниками техники безопасности. В статье произведен анализ производственного травматизма, определены причины производственного травматизма.
The article shows the expediency of preventing industrial injuries, which is based on the study and analysis of the causes of accidents and accidents, participation in their investigation and control over the implementation of safety measures by employees. The article analyzes industrial injuries, identifies the causes of industrial injuries.
MENTORING IN MEDICINE AND HEALTHCARE: RESIDENTS' OPINIONS
Authors: Лариса Николаевна Грибина, , Тамара Сергеевна Дьяченко, 
Published: December 2022

В статье представлены результаты социологического исследования отношения ординаторов первого года обучения к наставничеству в медицине. Большинство ординаторов (62,3%) осведомлены о существовании наставничества, только у 36,3% имеется наставник с первого дня на работе. По ответам респондентов составлен «портрет» наставника.
The article presents the results of a sociological study of the attitude of first-year residents to mentoring in medicine. The majority of residents (62.3%) are aware of the existence of mentoring, but only 36.3% of respondents indicated that they have a mentor from the first day on the job. Based on the responses of the respondents, a “portrait” of a mentor was compiled.
THE PHILOSOPHY OF METROLOGY
Authors: Арсен Николаевич Халатов, 
Published: December 2022

Предлагаемая концепция преподавания общеобразовательного предмета «Метрология, стандартизация и сертификация», раскрывает его философию, в процессе изучения которой, у молодёжи воспитывается критическое мышление, основанное на формировании духовных эталонов, что, наряду с освоением профессиональных компетенций, учит отличать наши базовые ценности от активно навязываемых эрзац- ценностей и суррогатов.
The proposed concept of teaching a general education subject "Methodology, standardization and certification" reveals its philosophy, in the process of studying which, critical thinking is brought up among young people based on the formation of spiritual standards, which, along with the development of professional competencies, teaches us to distinguish our basic values from actively imposed ersatz ones. values and surrogates.