bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between health insurance status, neighborhood deprivation, and treatment delays in women with breast cancer living in Georgia
Authors: Awan Sofia , Saini Geetanjali , Gogineni Keerthi , Luningham Justin M. , Collin Lindsay J. , Bhattarai Shristi , Aneja Ritu , Williams Courtney P. 
Published: July 2023

AbstractBackgroundLittle is known regarding the association between insurance status and treatment delays in women with breast cancer and whether this association varies by neighborhood socioeconomic deprivation status.MethodsIn this cohort study, we used medical record data of women diagnosed with breast cancer between 2004 and 2022 at two Georgia‐based healthcare systems. Treatment delay was defined as >90 days to surgery or >120 days to systemic treatment. Insurance coverage was categorized as private, Medicaid, Medicare, other public, or uninsured. Area deprivation index (ADI) was used as a proxy for neighborhood‐level socioeconomic status. Associations between delayed treatment and insurance status were analyzed using logistic regression, with an interaction term assessing effect modification by ADI.ResultsOf the 14,195 women with breast cancer, 54% were non‐Hispanic Black and 52% were privately insured. Compared with privately insured patients, those who were uninsured, Medicaid enrollees, and Medicare enrollees had 79%, 75%, and 27% higher odds of delayed treatment, respectively (odds ratio [OR]: 1.79, 95% confidence interval [CI]: 1.32–2.43; OR: 1.75, 95% CI: 1.43–2.13; OR: 1.27, 95% CI: 1.06–1.51). Among patients living in low–deprivation areas, those who were uninsured, Medicaid enrollees, and Medicare enrollees had 100%, 84%, and 26% higher odds of delayed treatment than privately insured patients (OR: 2.00, 95% CI: 1.44–2.78; OR: 1.84, 95% CI: 1.48–2.30; OR: 1.26, 95% CI: 1.05–1.53). No differences in the odds of delayed treatment by insurance status were observed in patients living in high‐deprivation areas.Discussion/ConclusionInsurance status was associated with treatment delays for women living in low‐deprivation neighborhoods. However, for women living in neighborhoods with high deprivation, treatment delays were observed regardless of insurance status.
P2X7‐Receptor Pathway Involvement in the Anti‐Inflammatory Activity of Medicinal Plants
Authors: Ortiz Sergio , Šavikin Katarina , Massicot France , Olivier Elodie , Dutot Melody , Rat Patrice , Deguin Brigitte , Gođevac Dejan , Menković Nebojša , Živković Jelena , Zdunić Gordana , Boutefnouchet Sabrina 
Published: July 2023

AbstractMedicinal plants used in European folk medicine attached to Lamiales, Gentianales or Asterales orders are used to treat inflammatory disorders. Many targets have been identified but to date, implication of purinergic receptor P2X7 activation has not yet been investigated. We managed to evaluate the protective effect on P2X7 activation by plant extracts used as anti‐inflammatory in European folk medicine by the YO‐PRO‐1 uptake dye in vitro bioassay. Results revealed that among our selected plants, species from Scrophularia and Plantago genus were able to decrease significantly P2X7 activation (>50 % at 0.1 and 1 μg/mL). UPLC/MS, dereplication and metabolomic analysis of Scrophularia extracts, allowed us to identify the cinnamoyl‐iridoid harpagoside as putative inhibitor of P2X7 activation. These results open a new research field regarding the anti‐inflammatory mechanism of cinnamoyl‐iridoids bearing plants, which may involve the P2X7 receptor.
Facile Synthesis of Hybrid Nanoflowers Using Glycine and Phenylalanine and Investigation of Their Catalytic Activity
Authors: Demirbas Ayse , Karsli Baris , Ocsoy Ismail 
Published: July 2023

AbstractIn the context of the proposed work, two different amino acids (Glycine, Phenylalanine) have interacted with copper ions in a phosphate buffer (PBS) in place of enzymes. This interaction resulted in the nucleation of copper phosphate crystals and the formation of flower‐shaped amino acid‐copper hybrid nanostructures (AA‐hNFs), which grew through self‐assembly. While Cu (II) ions in the structure of AA‐hNFs were used as Fenton's agent for the catalytic activity. SEM, energy dispersive X‐ray spectroscopy, X‐ray diffraction, and Fourier transform infrared spectroscopy measurements were used to define the AA‐hNFs′ characterisation. The peroxidase‐like activities of AA‐hNFs were investigated by UV/VIS spectrophotometer. Metal nanoparticles have peroxidase‐like activity. A class of enzymes known as peroxidases is able to catalyze the conversion of hydrogen peroxide into hydroxyl radicals. These radicals also take part in electron transfers with substrates, which results in color during oxidation. When cupric oxide nanoparticles are added to the peroxidase substrate while H2O2is present, a blue color product with a maximum absorbance at=652 nm can result, demonstrating the catalytic activity of a peroxidase. The morphology and composition of AA‐hNFs were carefully characterized and the synthesized parameters were optimized systematically. Results showed that the nanoparticles were dispersed with an average diameter of 7–9 μm and indicated a uniform flower shape. The results of the investigation are anticipated to significantly advance a number of technical and scientific sectors.
Should CINAHL be used as one of the main databases for evidence synthesis of health services intervention?
Authors: Dhippayom Teerapon , Rattanachaisit Natnicha , Wateemongkollert Apinya , Napim Rawiwan , Chaiyakunapruk Nathorn 
Published: July 2023

AbstractIntroductionCINAHL is not listed as one of the minimum databases for systematic review (SR) of interventions in the Methodological Expectations of the Cochrane Intervention Review.ObjectiveTo determine additional studies uniquely identified from the CINAHL search in SR of health services interventions (HSI).MethodsWe searched PubMed from inception to October 1, 2022 to identify SRs of HSI that determined clinical or humanistic outcomes of HSI and used CINAHL. Out of 5655 Systematic reviews identified, we randomly selected 374 SRs and extracted all primary studies included. We then explored the bibliographic databases in which the journals of those studies were indexed. The outcome of interest was the number of studies uniquely available in CINHAL. We also performed a subgroup analysis based on the type of HSI. We performed descriptive statistics to report the study outcomes using Excel (Microsoft 365).ResultsA total of 7550 primary studies were identified from the 374 Systematic reviews that met the inclusion criteria. Of these studies, 7380 were journal publications that have been indexed in MEDLINE/PubMed (75.1%), Scopus (74.5%), Sciences Citation Index, SCI (54.7%), Embase (48.1%), and CINAHL (34.9%). Only 83 out of 7380 (1.1%) studies were published in journals that were uniquely indexed in CINAHL. The percentage of studies that were only available in other databases was 9.7% (Scopus), 4.3% (MEDLINE/PubMed), 1.6% (SCI), and 0.3% (Embase). The number of studies that were unique to CINAHL in specific types of HSI were: 24/1570 (1.5%) for community health services, 20/1520 (1.3%) for preventive health services, 45/3624 (1.2%) for patient care, 8/1173 (0.7%) for mental health services, and 18/2804 (0.6%) for rehabilitation.ConclusionThe gain of CINAHL to identify unique primary studies for SR of HSI appears minimal. The impact of missing studies uniquely available in CINAHL on SR summary or magnitude of effect estimates from meta‐analysis requires further investigation.
Current Status of Weekly Insulin Analogs and Their Pharmacokinetic/Pharmacodynamic Evaluation by the Euglycemic Clamp Technique
Authors: An Na , Wang Xuhong , He Anshun , Chen Wei 
Published: July 2023

AbstractDiabetes mellitus represents a significant global health threat characterized by hyperglycemia caused by inadequate insulin secretion and/or insulin resistance. Exogenous insulin supplements had been recognized as a crucial treatment for achieving successful glycemic control in patients with Type 1 and most patients with Type 2 diabetes. Over the past century, substantial progress has been made in the development of novel insulin formulations, including the super‐fast‐acting and long‐acting basal insulin analogs, of which the latter is indispensable for the management of nocturnal fasting and intraprandial blood glucose within the normal physiological range. Recently, combining chemical and genetic engineering with drug optimization have resulted in a formidable evolution in ultra‐long‐acting weekly insulin. Here, the current state of once‐weekly insulin analogs and the euglycemic clamp technique used in the early clinical development to elucidate the pharmacokinetics and pharmacodynamics of this type of novel weekly insulin analogs were systematically overviewed.
Isolation of Adult Mouse Cardiac Fibroblasts
Authors: Almazloum Abir , Khalil Hadi 
Published: July 2023

AbstractFibroblasts are dynamic cells of mesenchymal origin that regulate tissue homeostasis, extracellular matrix production, and acute wound healing. Fibroblasts respond to tissue injury and inflammation by differentiating into myofibroblasts and secreting extracellular matrix proteins. Fibroblasts are the principal mediators of the fibrotic response in all tissues and organs. Adult primary fibroblasts represent an essential tool for in vitro studies. Although they lack surface markers, fibroblasts are relatively easy to obtain and culture; primary fibroblasts are sensitive heterogeneous cell subpopulations with limited expansion potential and increased differentiation capacity. Adult primary fibroblasts fail to maintain an undifferentiated state ex vivo for long periods and quickly differentiate into myofibroblasts in culture, which necessitates the utilization of these cells either directly after isolation or after a few passages. Herein, we describe a detailed protocol for enzymatic isolation of primary cardiac fibroblasts from adult mouse hearts and their culture and expansion in a serum‐containing culture medium. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Preparation of primary adult cardiac fibroblasts for culture and single‐cell analysis
Psychophysiological disorders and the skin
Authors: Carniciu Simona , Jafferany Mohammad 
Published: July 2023

AbstractThe interaction between the mind, skin, and body is well studied lately and many studies focus on the multiple mechanisms between them that affects skin health. From psychology, psychoneuroimmunology, and immunology, to psychophysiological and inflammation, there are many issues to consider for the brain–skin cross‐interaction. Altered balance or activation of the neuroendocrine‐immune system triggers a cascade of events that allow the so‐called fight–flight reaction that influences several skin diseases, as a risk factor for developing a dermatological disease or disease flares. Psychosocial stressors change the responses of neuroactive substances that regulates the immune system. Research focuses on study of several influencers like neuroendocrine mediators (corticotrophin‐releasing factor, substance P, adrenocorticotrophic hormone, glucocorticoids, and catecholamines), pro‐inflammatory cytokines (IFN‐g, IL‐2, IL‐4, IL‐5, IL‐10, IL‐13), the activity of mast cells, NK cells or dendritic cells in the skin. The conditions exacerbated by psychological factors and stress are seborrheic dermatitis, psoriasis, acne, rosacea, atopic dermatitis, urticaria, zona zoster, herpes simplex, neurodermatitis, hyperhidrosis, allodynia.
Urban habitat fragmentation and floral resources shape the occurrence of gut parasites in two bumblebee species
Authors: Tommasi Nicola , Colombo Beatrice , Pioltelli Emiliano , Biella Paolo , Casiraghi Maurizio , Galimberti Andrea 
Published: July 2023

AbstractUrbanization and the expansion of human activities foster radical ecosystem changes with cascading effects also involving host‐pathogen interactions. Urban pollinator insects face several stressors related to landscape and local scale features such as green habitat loss, fragmentation and availability reduction of floral resources with unpredictable effects on parasite transmission. Furthermore, beekeeping may contribute to the spread of parasites to wild pollinators by increasing the number of parasite hosts. Here we used DNA‐based diagnostics tools to evaluate how the occurrence of parasites, namely microsporidians (Nosema spp.), trypanosomatids (Crithidia spp.) and neogregarines (Apicystis bombi), is shaped by the above‐mentioned stressors in two bumblebee species (i.e. Bombus terrestris and Bombus pascuorum). Infection rates of the two species were different and generally higher in B. terrestris. Moreover, they showed different responses towards the same ecological variables, possibly due to differences in body size and foraging habits supposed to affect their susceptibility to parasite infection. The probability of infection was found to be reduced in B. pascuorum by green habitat fragmentation, while increased along with floral resource availability. Unexpectedly, B. terrestris had a lower parasite richness nearby apiaries maybe due to the fact that parasites are prone to be transmitted among the most abundant species. Our finding supports the need to design proper conservation measures based on species‐specific knowledge, as suggested by the variation in the parasite occurrence of the two species. Moreover, conservation policies aiming at safeguarding pollinators through flower planting should consider the indirect effects of these measures for parasite transmission together with pollinator biodiversity issues.
Tend‐and‐befriend and rally around the flag effects during the COVID‐19 pandemic: Differential longitudinal change patterns in multiple aspects of social cohesion
Authors: Silveira Sarita , Hecht Martin , Voelkle Manuel C. , Singer Tania 
Published: July 2023

AbstractThe COVID‐19 pandemic and its lockdowns have uniquely challenged our social lives. The current study seeks to explore changes in social cohesion on various psychological dimensions (trust, belonging, social interaction, social engagement) and social system levels (family, friends, neighbours, institutions, nations), assessed in 3522 Berlin residents before, during, and after the first lockdown, and four times during the second lockdown. The first lockdown had a consistently negative impact on psychological dimensions of social cohesion. However, the picture was more nuanced regarding social systems: micro‐level cohesion with family members and neighbours, and macro‐level processes of institutional trust and national and international belonging increased, particularly in women. This suggests tend‐and‐befriend tendencies of affiliation, and affirmation of political and national identities. However, social cohesion collapsed during the second lockdown, indicating pandemic fatigue effects. Findings suggest a multidimensional approach to social cohesion and highlight the relevance of stressor duration in times of collective crisis.
Templated insertions—DNA repair gets acrobatic
Authors: Stroik Susanna , Luthman Adam J. , Ramsden Dale A. 
Published: July 2023

AbstractDeletions associated with the repair of DNA double‐strand breaks is a source of genetic alternation and a recognized source of disease‐causing mutagenesis. Theta‐mediated end joining is a DNA repair mechanism, which guarantees deletions by its employment of microhomology (MH) alignment to facilitate end joining. A lesser‐characterized templated insertion ability of this pathway, on the other hand, is associated with both deletion and insertion. This mechanism is characterized by at least one round of polymerase θ‐mediated synthesis, which does not result in successful repair, followed by a subsequent round of polymerase engagement and synthesis that does lead to repair. Here we focus on the mechanisms by which polymerase θ introduces these insertions—direct, inverse, and a new class which we have termed strand switching. We observe this new class of templated insertions at multiple loci and across multiple species, often at a comparable frequency to those previously characterized. Templated insertion mutations are often enriched in cancer genomes and repeat expansion disorders. This repair mechanism thus contributes to disease‐associated mutagenesis, and may plausibly even promote disease. Characterization of the types of polymerase θ‐dependent insertions can provide new insight into these diseases and clinical promise for treatment.
Modeling the Partitioning of Anionic Carboxylic and Perfluoroalkyl Carboxylic and Sulfonic Acids to Octanol and Membrane Lipid
Authors: Torralba-Sanchez Tifany L. , Di Toro Dominic M. , Dmitrenko Olga , Murillo-Gelvez Jimmy , Tratnyek Paul G. 
Published: July 2023

Abstract
Perfluoroalkyl carboxylic and sulfonic acids (PFCAs and PFSAs, respectively) have low acid dissociation constant values and are, therefore, deprotonated under most experimental and environmental conditions. Hence, the anionic species dominate their partitioning between water and organic phases, including octanol and phospholipid bilayers which are often used as model systems for environmental and biological matrices. However, data for solvent–water (SW) and membrane–water partition coefficients of the anion species are only available for a few per- and polyfluoroalkyl substances (PFAS). In the present study, an equation is derived using a Born-Haber cycle that relates the partition coefficients of the anions to those of the corresponding neutral species. It is shown via a thermodynamic analysis that for carboxylic acids (CAs), PFCAs, and PFSAs, the log of the solvent–water partition coefficient of the anion, log KSW(A−), is linearly related to the log of the solvent–water partition coefficient of the neutral acid, log KSW(HA), with a unity slope and a solvent-dependent but solute-independent intercept within a PFAS (or CA) family. This finding provides a method for estimating the partition coefficients of PFCAs and PFSAs anions using the partition coefficients of the neutral species, which can be reliably predicted using quantum chemical methods. In addition, we have found that the neutral octanol–water partition coefficient, log KOW, is linearly correlated to the neutral membrane–water partition coefficient, log KMW; therefore, log KOW, being a much easier property to estimate and/or measure, can be used to predict the neutral log KMW. Application of this approach to KOW and KMW for PFCAs and PFSAs demonstrates the utility of this methodology for evaluating reported experimental data and extending anion property data for chain lengths that are unavailable. Environ Toxicol Chem 2023;42:2317–2328. © 2023 SETAC
Bioinspired gradient scaffolds for osteochondral tissue engineering
Authors: Peng Yachen , Zhuang Yaling , Liu Yang , Le Hanxiang , Li Di , Zhang Mingran , Liu Kai , Zhang Yanbo , Zuo Jianlin , Ding Jianxun 
Published: July 2023

AbstractRepairing articular osteochondral defects present considerable challenges in self‐repair due to the complex tissue structure and low proliferation of chondrocytes. Conventional clinical therapies have not shown significant efficacy, including microfracture, autologous/allograft osteochondral transplantation, and cell‐based techniques. Therefore, tissue engineering has been widely explored in repairing osteochondral defects by leveraging the natural regenerative potential of biomaterials to control cell functions. However, osteochondral tissue is a gradient structure with a smooth transition from the cartilage to subchondral bone, involving changes in chondrocyte morphologies and phenotypes, extracellular matrix components, collagen type and orientation, and cytokines. Bioinspired scaffolds have been developed by simulating gradient characteristics in heterogeneous tissues, such as the pores, components, and osteochondrogenesis‐inducing factors, to satisfy the anisotropic features of osteochondral matrices. Bioinspired gradient scaffolds repair osteochondral defects by altering the microenvironments of cell growth to induce osteochondrogenesis and promote the formation of osteochondral interfaces compared with homogeneous scaffolds. This review outlines the meaningful strategies for repairing osteochondral defects by tissue engineering based on gradient scaffolds and predicts the pros and cons of prospective translation into clinical practice.
Karyotype diversity in four Terminalia L. species and its taxonomic implications
Authors: Akter Suma , Dash Chandan Kumar , Sultana Syeda Sharmeen 
Published: July 2023

AbstractFour Terminalia L. species namely, T. arjuna (Roxb.) Wight and Arn., T. bellirica (Gaertn.) Roxb., T. chebula Retz. and T. catappa L. were studied to evaluate the chromosomal relationship corresponding with their taxonomic characters. Analysis of mitotic metaphases showed that the somatic chromosome numbers and the centromeric formulas of these species were 2n = 24 (16m + 8sm) for T. arjuna, 2n = 48 (36m + 12sm) for T. bellirica, 2n = 48 (46m + 2sm) for T. chebula and 2n = 24 (24m) for T. catappa. Two pairs of satellites were found in T. bellirica and T. catappa. Based on different karyological data, T. chebula was positioned in 1A, T. arjuna and T. catappa in 1B and T. bellirica in 2B. Based on comparative karyomorphological data, T. bellirica and T. arjuna presented more advanced features in comparison with T. chebula and T. catappa, which also correlated with the taxonomic characters in respect of the evolutionary point of view. Therefore, the present investigation effectively evaluated chromosomal relationships with morphological implications that conferred upon evolutionary changes among these four Terminalia species.
Surgery‐ineligible elderly patients with cutaneous squamous cell carcinoma of the head and neck region gain clinical benefit from definitive weekly hypofractionated radiotherapy
Authors: De Felice Francesca , Serpone Maria , Cattaneo Carlo Guglielmo , Di Giammarco Federico , Fallico Alberto , Delle Donne Alessia , Lanzilao Maura , Vitti Elisa , Marampon Francesco , Musio Daniela , Tombolini Vincenzo , Minniti Giuseppe 
Published: July 2023

AbstractBackgroundTo evaluate the role of definitive weekly hypofractionated radiotherapy (RT) for the treatment of surgery‐ineligible elderly patients with cutaneous squamous cell carcinoma of the head and neck region (cHNSCC).MethodsEligible elderly patients (aged ≥75 years) with cHNSCC were included. Patients received definitive weekly hypofractionated RT, using megavoltage electrons, to a total dose of 56–64 Gy (8 Gy per fraction). Primary endpoint was objective response rate (ORR), defined as the percentage of patients with a complete (CR) or partial response (PR). Secondary endpoints included duration of response (DOR), progression‐free survival (PFS), overall survival (OS), pain response, tolerability, and safety.ResultsA total of 19 patients with 27 lesions were included and treated with definitive weekly hypofractionated RT. All patients received the prescribed total dose. ORR was 92.6%, including 70.4% of lesions with a CR and 22.2% with a PR. Median DOR was 12 months. No severe toxicity occurred.ConclusionsOur study confirmed the satisfying efficacy and acceptable toxicity of definitive weekly hypofractionated RT for cHNSCC in elderly patients. Our results establish weekly hypofractionated scheduleas a promising treatment option for elderly patients with cHNSCC.