bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serine protease PRSS56, a novel cancer-testis antigen activated by DNA hypomethylation, promotes colorectal and gastric cancer progression via PI3K/AKT axis
Authors: Li Dandan , Xia Lingyun , Huang Pan , Wang Zidi , Guo Qiwei , Huang Congcong , Leng Weidong , Qin Shanshan 
Published: July 2023

Abstract
Background
Cancer/testis (CT) antigens/genes are usually overexpressed in cancers and exhibit high immunogenicity, making them promising targets for immunotherapy and cancer vaccines. The role of serine protease PRSS56 in cancers remains unknown to date.

Methods
RNA sequencing studies were performed to screen CT genes in gastric cancer (GC) and colorectal cancer (CRC) cells exposed to DNA methyltransferase inhibitor 5-aza-2’-deoxycytidine (5-AZA-CdR). Bioinformatics analysis was conducted to analyze the correlation between PRSS56 expression and DNA methylation. Functional experiments were performed to explore the biological function of PRSS56 in GC and CRC.

Results
In this study, we identified the testis-specific serine proteases PRSS56 as a novel CT antigen. PRSS56 was frequently overexpressed in various cancers, especially in gastrointestinal cancer. PRSS56 expression was negatively associated with promoter DNA methylation level, and positively associated with gene body methylation level. PRSS56 expression was significantly activated in colorectal and gastric cancer cells exposed to DNA methyltransferase inhibitors. Importantly, our finding highlights that the decreased methylation level of the CpG site cg10242318 in the PRSS56 promoter region resulted in its overexpression in GC and CRC. Additionally, functional assays verified that PRSS56 overexpression activated PI3K-AKT signaling in GC and CRC.

Conclusion
Serine protease PRSS56 is a novel CT antigen that is reactivated in cancers by promoter DNA hypomethylation. PRSS56 functions oncogenic roles in GC and CRC by activating of PI3K/AKT axis. Our results presented here represent the first data on the function of the serine protease PRSS56 in cancers.
ER Ca2+ overload activates the IRE1α signaling and promotes cell survival
Authors: Zhao Song , Feng Haiping , Jiang Dongfang , Yang Keyan , Wang Si-Tong , Zhang Yu-Xin , Wang Yun , Liu Hongmei , Guo Caixia , Tang Tie-Shan 
Published: July 2023

Abstract
Background
Maintaining homeostasis of Ca2+ stores in the endoplasmic reticulum (ER) is crucial for proper Ca2+ signaling and key cellular functions. Although Ca2+ depletion has been known to cause ER stress which in turn activates the unfolded protein response (UPR), how UPR sensors/transducers respond to excess Ca2+ when ER stores are overloaded remain largely unclear.

Results
Here, we report for the first time that overloading of ER Ca2+ can directly sensitize the IRE1α-XBP1 axis. The overloaded ER Ca2+ in TMCO1-deficient cells can cause BiP dissociation from IRE1α, promote the dimerization and stability of the IRE1α protein, and boost IRE1α activation. Intriguingly, attenuation of the over-activated IRE1α-XBP1s signaling by a IRE1α inhibitor can cause a significant cell death in TMCO1-deficient cells.

Conclusions
Our data establish a causal link between excess Ca2+ in ER stores and the selective activation of IRE1α-XBP1 axis, underscoring an unexpected role of overload of ER Ca2+ in IRE1α activation and in preventing cell death.
Stimulation strategies for electrical and magnetic modulation of cells and tissues
Authors: Omer Suleyman A. , McKnight Kaitlyn H. , Young Lucas I. , Song Shang 
Published: July 2023

AbstractElectrical phenomena play an important role in numerous biological processes including cellular signaling, early embryogenesis, tissue repair and remodeling, and growth of organisms. Electrical and magnetic effects have been studied on a variety of stimulation strategies and cell types regarding cellular functions and disease treatments. In this review, we discuss recent advances in using three different stimulation strategies, namely electrical stimulation via conductive and piezoelectric materials as well as magnetic stimulation via magnetic materials, to modulate cell and tissue properties. These three strategies offer distinct stimulation routes given specific material characteristics. This review will evaluate material properties and biological response for these stimulation strategies with respect to their potential applications in neural and musculoskeletal research.
Different expression and prognostic value of troponin in ischemic cardiomyopathy and non-ischemic dilated cardiomyopathy
Authors: Gao Wei , Zhang Meng , Song Yu , Han Xueting , Xu Yamei , Zhou Jingmin , Ge Junbo 
Published: July 2023

Abstract
Background
Early risk stratification of patients with ischemic cardiomyopathy (ICM) and non-ischemic dilated cardiomyopathy (NIDCM) may be beneficial for therapies.

Methods
We retrospectively enrolled all patients admitted for acute heart failure (HF) between January 2019 and December 2021 in Zhongshan Hospital Fudan University, dividing them according to etiology (ICM or NIDCM). Cardiac troponin T (TNT) concentration was compared between two groups. Risk factors for positive TNT and in-hospital all-cause mortality were investigated with regression analysis.

Results
A total of 1525 HF patients were enrolled, including 571 ICM and 954 NIDCM. The TNT positive patients were not different between the two groups (41.3% in ICM group vs. 37.8% in NIDCM group, P = 0.215). However, the TNT value in ICM group were significantly higher than that in NIDCM group (0.025 (0.015–0.053) vs. 0.020 (0.014–0.041), P = 0.001). NT-proBNP was independently associated with TNT in both ICM and NIDCM group. Although the in-hospital all-cause mortality did not show much difference between the two groups (1.1% vs. 1.9%, P = 0.204), the NIDCM diagnosis was associated with reduced risk of mortality after multiple adjustments (OR 0.169, 95% CI 0.040–0.718, P = 0.016). Other independent risk factors included the level of NT-proBNP (OR 8.260, 95% CI 3.168–21.533, P < 0.001), TNT (OR 8.118, 95% CI 3.205–20.562, P < 0.001), and anemia (OR 0.954, 95% CI 0.931–0.978, P < 0.001). The predictive value of TNT and NT-proBNP for all-cause mortality was similar. However, the best cutoff values of TNT for mortality were different between ICM and NIDCM groups, which were 0.113 ng/mL and 0.048 ng/mL, respectively.

Conclusion
The TNT level was higher in ICM patient than that in NIDCM patients. TNT was an independent risk factor for in-hospital all-cause mortality for both ICM and NIDCM patients, although the best cutoff value was higher in ICM patients.
Adiposity and lipid metabolism indicators mediate the adverse effect of glucose metabolism indicators on oogenesis and embryogenesis in PCOS women undergoing IVF/ICSI cycles
Authors: Jiang Huahua , Si Manfei , Tian Tian , Shi Huifeng , Huang Ning , Chi Hongbin , Yang Rui , Long Xiaoyu , Qiao Jie 
Published: July 2023

Abstract
Background
Polycystic ovary syndrome (PCOS) women have high incidences of dyslipidemia, obesity, impaired glucose tolerance (IGT), diabetes, and insulin resistance (IR) and are fragile to female infertility. Obesity and dyslipidemia may be the intermediate biological mechanism for the associations between glucose metabolism dysfunction and abnormal oogenesis and embryogenesis.

Methods
This retrospective cohort study was performed at a university-affiliated reproductive center. A total of 917 PCOS women aged between 20 and 45 undergoing their first IVF/ICSI embryo transfer cycles from January 2018 to December 2020 were involved. Associations between glucose metabolism indicators, adiposity and lipid metabolism indicators, and IVF/ICSI outcomes were explored using multivariable generalized linear models. Mediation analyses were further performed to examine the potential mediation role of adiposity and lipid metabolism indicators.

Results
Significant dose-dependent relationships were found between glucose metabolism indicators and IVF/ICSI early reproductive outcomes and between glucose metabolism indicators and adiposity and lipid metabolism indicators (all P < 0.05). Also, we found significant dose-dependent relationships between adiposity and lipid metabolism indicators and IVF/ICSI early reproductive outcomes (all P < 0.05). The mediation analysis indicated that elevated FPG, 2hPG, FPI, 2hPI, HbA1c, and HOMA2-IR were significantly associated with decreased retrieved oocyte count, MII oocyte count, normally fertilized zygote count, normally cleaved embryo count, high-quality embryo count, or blastocyst formation count after controlling for adiposity and lipid metabolism indicators. Serum TG mediated 6.0–31.0% of the associations; serum TC mediated 6.1–10.8% of the associations; serum HDL-C mediated 9.4–43.6% of the associations; serum LDL-C mediated 4.2–18.2% of the associations; and BMI mediated 26.7–97.7% of the associations.

Conclusions
Adiposity and lipid metabolism indicators (i.e., serum TG, serum TC, serum HDL-C, serum LDL-C, and BMI) are significant mediators of the effect of glucose metabolism indicators on IVF/ICSI early reproductive outcomes in PCOS women, indicating the importance of preconception glucose and lipid management and the dynamic equilibrium of glucose and lipid metabolism in PCOS women.
Schisandrin A regulates the Nrf2 signaling pathway and inhibits NLRP3 inflammasome activation to interfere with pyroptosis in a mouse model of COPD
Authors: Zeng Jiamin , Liao Sida , Liang Zhu , Li Caiping , Luo Yuewen , Wang Kexin , Zhang Dapeng , Lan Lan , Hu Suzhen , Li Wanyan , Lin Ran , Jie Zichen , Hu Yuanlong , Dai Shiting , Zhang Zhimin 
Published: July 2023

AbstractChronic obstructive pulmonary disease (COPD) is a serious chronic lung disease. Schisandrin A (SchA) is one of the most important active ingredients inSchisandra chinensisand has been used to treat various lung diseases in several countries. Here, we studied the pharmacological effect of SchA on airway inflammation induced by cigarette smoke (CS) and explored the therapeutic mechanism of SchA in COPD model mice. Our results showed that SchA treatment significantly improved the lung function of CS-induced COPD model mice and reduced the recruitment of leukocytes and hypersecretion of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) in bronchoalveolar lavage fluid (BALF). H&E staining showed that SchA treatment could effectively reduce emphysema, immune cell infiltration and airway wall destruction. In addition, we found that SchA treatment can stimulate the expression of heme oxygenase-1 (HO-1) through the nuclear factor-erythroid 2-related factor (Nrf2) pathway, significantly reduce oxidative stress, increase catalase (CAT) and superoxide dismutase (SOD) levels, and suppress the level of malondialdehyde (MDA) in COPD model mice. Moreover, SchA treatment suppressed the generation of the NLRP3/ASC/Caspase1 inflammasome complex to inhibit the inflammatory response caused by IL-1β and IL-18 and pyroptosis caused by GSDMD. In conclusion, our study shows that SchA treatment can inhibit the production of ROS and the activation of the NLRP3 inflammasome by upregulating Nrf-2, thereby producing anti-inflammatory effects and reducing lung injury in COPD model mice. More importantly, SchA exhibited similar anti-inflammatory effects to dexamethasone in COPD model mice, and we did not observe substantial side effects of SchA treatment. The high safety of SchA makes it a potential candidate drug for the treatment of COPD.
TiNbSn stems with gradient changes of Young’s modulus and stiffness reduce stress shielding compared to the standard fit-and-fill stems
Authors: Baba Kazuyoshi , Mori Yu , Chiba Daisuke , Kuwahara Yoshiyuki , Kurishima Hiroaki , Tanaka Hidetatsu , Kogure Atsushi , Kamimura Masayuki , Yamada Norikazu , Ohtsu Susumu , Oyama Masamizu , Masahashi Naoya , Hanada Shuji , Itoi Eiji , Aizawa Toshimi 
Published: July 2023

Abstract
Background
The difference between Young’s moduli of the femur and the stem causes stress shielding (SS). TiNbSn (TNS) stem has a low Young’s modulus and strength with gradient functional properties during the change in elastic modulus with heat treatment. The aim of this study was to investigate the inhibitory effect of TNS stems on SS and their clinical outcomes compared to conventional stems.

Methods
This study was a clinical trial. Primary THA was performed using a TNS stem from April 2016 to September 2017 for patients in the TNS group. Unilateral THA was performed using a Ti6Al4V alloy stem from January 2007 to February 2011 for patients in the control group. The TNS and Ti6Al4V stems were matched in shape. Radiographs were obtained at the 1- and 3-year follow-ups. Two surgeons independently checked the SS grade and appearance of cortical hypertrophy (CH). The Japanese Orthopaedic Association (JOA) scores before and 1 year after surgery were assessed as clinical scores.

Results
None of the patients in the TNS group had grade 3 or 4 SS. In contrast, in the control group, 24% and 40% of patients had grade 3 and 4 SS at the 1- and 3-year follow-ups, respectively. The SS grade was lower in the TNS group than in the control group at the 1- and 3-year follow-ups (p < 0.001). The frequencies of CH in both groups were no significant difference at the 1- and 3-year follow-ups. The JOA scores of the TNS group significantly improved at 1 year after surgery and were comparable to control group.

Conclusion
The TNS stem reduced SS at 1 and 3 years after THA compared to the proximal-engaging cementless stem, although the shapes of the stems matched. The TNS stem could reduce SS, stem loosening, and periprosthetic fractures.
Trial registration: Current Controlled Trials. ISRCTN21241251. https://www.isrctn.com/search?q=21241251. The date of registration was October 26, 2021. Retrospectively registered.
Biocultural mapping: unpacking the myth of an unsuitable Country in the arid zone, Willandra Lakes Region World Heritage Area, Australia
Authors: Thomas Katherine 
Published: July 2023

AbstractDefining spaces and places in retrojective Geographical Information Systems (GIS) of land use and occupancy is not an easy task. Research into the areas described as suitable for land use and occupancy need to incorporate multiple perspectives of what makes a land use patch useful or salient ecologically. The effect of the concept of 'Terra Nullius' and European colonisation is deeply apparent in the current GIS models of historical land use and occupancy of Aboriginal communities within arid zones in Australia. Biocultural zones of land use and occupancy zones omit spaces and places of habitation due to European bias of what a suitable ecological or hydrological land use zone should look like. This article employs Exploratory GIS methods to interrogate the data layers within the Willandra Lakes Region World Heritage Area, NSW, Australia. This work conclusively demonstrates that there are ranges of areas and land suitability zones prior to colonisation in the nineteenth century. In turn, these Exploratory GIS models of an active Country comprehensively address the question of why visually salient areas of hydrological and ecological Indigenous land use and occupancy continue to be ignored, destroyed, and damaged by settlements in semi-arid regions. Biocultural GIS mapping unpacks the myth that areas were empty or uninhabited by Aboriginal communities and underlines the need for biocultural GIS mapping tools to understand the habitable spaces and places of the arid zone.
Affordability of healthy diets is associated with increased food systems performance in Nigeria: state-level analysis
Authors: Mekonnen Daniel A. , Adeyemi Olutayo , Gilbert Rachel , Akerele Dare , Achterbosch Thom , Herforth Anna 
Published: July 2023

AbstractThis study examines the relationships between healthy diets’ affordability and food systems performance across Nigerian states. On a composite index (FSI) constructed from key food system components, states in the southern zones perform relatively better than those in the north, whereas a higher share of households who cannot afford a healthy diet was from northern states. Findings suggest the need for more focused attention on resilience building for improved food systems especially in northern Nigeria. Results also indicate cases where unaffordability of a healthy diet remains high in the face of comparatively lower cost and vice versa, highlighting the need for more efficient and equitable food supply systems. Findings of this study further suggest that achieving affordable healthy diets would require stepping up actions to improve, among others, access to basic services (such as good roads and electricity), increased presence of financial services (such as rural banks), greater access to information and communications services (mobile phone subscription rates and increased radio/television station coverage), facilitating more favorable and predictable business environment, and promoting women’s empowerment.
Rhizobial nitrogen fixation efficiency shapes endosphere bacterial communities and Medicago truncatula host growth
Authors: Lagunas Beatriz , Richards Luke , Sergaki Chrysi , Burgess Jamie , Pardal Alonso Javier , Hussain Rana M. F. , Richmond Bethany L. , Baxter Laura , Roy Proyash , Pakidi Anastasia , Stovold Gina , Vázquez Saúl , Ott Sascha , Schäfer Patrick , Gifford Miriam L. 
Published: July 2023

Abstract
Background
Despite the knowledge that the soil–plant–microbiome nexus is shaped by interactions amongst its members, very little is known about how individual symbioses regulate this shaping. Even less is known about how the agriculturally important symbiosis of nitrogen-fixing rhizobia with legumes is impacted according to soil type, yet this knowledge is crucial if we are to harness or improve it. We asked how the plant, soil and microbiome are modulated by symbiosis between the model legume Medicago truncatula and different strains of Sinorhizobium meliloti or Sinorhizobium medicae whose nitrogen-fixing efficiency varies, in three distinct soil types that differ in nutrient fertility, to examine the role of the soil environment upon the plant–microbe interaction during nodulation.

Results
The outcome of symbiosis results in installment of a potentially beneficial microbiome that leads to increased nutrient uptake that is not simply proportional to soil nutrient abundance. A number of soil edaphic factors including Zn and Mo, and not just the classical N/P/K nutrients, group with microbial community changes, and alterations in the microbiome can be seen across different soil fertility types. Root endosphere emerged as the plant microhabitat more affected by this rhizobial efficiency-driven community reshaping, manifested by the accumulation of members of the phylum Actinobacteria. The plant in turn plays an active role in regulating its root community, including sanctioning low nitrogen efficiency rhizobial strains, leading to nodule senescence in particular plant–soil–rhizobia strain combinations.

Conclusions
The microbiome–soil–rhizobial dynamic strongly influences plant nutrient uptake and growth, with the endosphere and rhizosphere shaped differentially according to plant–rhizobial interactions with strains that vary in nitrogen-fixing efficiency levels. These results open up the possibility to select inoculation partners best suited for plant, soil type and microbial community.
Reduction of oxytocin-containing neurons and enhanced glymphatic activity in the hypothalamic paraventricular nucleus of patients with type 2 diabetes mellitus
Authors: Correa-da-Silva Felipe , Kalsbeek Martin J. , Gadella Femke S. , Oppersma Jorn , Jiang Wei , Wolff Samantha E. C. , Korpel Nikita L. , Swaab Dick F. , Fliers Eric , Kalsbeek Andries , Yi Chun-Xia 
Published: July 2023

AbstractEvidence from animal experiments has shown that the hypothalamic paraventricular nucleus (PVN) plays a key role in regulating body weight and blood glucose levels. However, it is unclear whether neuron populations in the human PVN are involved in the development of type 2 diabetes mellitus (T2DM). To address this, we investigated the neuronal and glial populations in the PVN of 26 T2DM patients and 20 matched controls. Our findings revealed a significant reduction in oxytocin (Oxt) neuron density in the PVN of T2DM patients compared to controls, while other neuronal populations remained unchanged. This suggests that Oxt neurons may play a specific role in the pathophysiology of T2DM. Interestingly, the reduction in Oxt neurons was accompanied by a decreased melanocortinergic input in to the PVN as reflected by a reduction in alpha-MSH immunoreactivity. We also analysed two glial cell populations, as they are important for maintaining a healthy neural microenvironment. We found that microglial density, phagocytic capacity, and their proximity to neurons were not altered in T2DM patients, indicating that the loss of Oxt neurons is independent of changes in microglial immunity. However, we did observe a reduction in the number of astrocytes, which are crucial for providing trophic support to local neurons. Moreover, a specific subpopulation of astrocytes characterized by aquaporin 4 expression was overrepresented in T2DM patients. Since this subset of astrocytes is linked to the glymphatic system, their overrepresentation might point to alterations in the hypothalamic waste clearance system in T2DM. Our study shows selective loss of Oxt neurons in the PVN of T2DM individuals in association with astrocytic reduction and gliovascular remodelling. Therefore, hypothalamic Oxt neurons may represent a potential target for T2DM treatment modalities.
Multi combination pattern labeling by using deep learning for chameleon rotary machine environment
Authors: Kang JiEun , Kim SuBi , Yoon YongIk 
Published: July 2023

AbstractRotary machines are constructed and operated in diverse industrial environments and operate according to various specifications and characteristics. When rotary machinery constructed under dynamic real world environments is in operation, various types of vibrations are generated depending on the normal or defective state of the machinery. In this way, Numerous studies have been conducted on vibration analysis for diagnosing the state of rotary machinery. However, Without performing robust data cleansing and comprehensive labeling of the internal and external state of complex machinery, the analysis process of the condition monitoring system faces difficulties in accurately identifying the various and complex states of rotary machines and making decisions in the dynamic real world. To overcome these limitations, this paper proposes Multi Combination Pattern Labeling (MCPL) method. By simultaneously considering the complex internal and external states of rotary machines, MCPL generates detailed vibration frequency pattern criteria and labels for each state. Based on these complex pattern classifications, it is able to classify various types of abnormal states. The MCPL generates FFT patterns and spectrogram patterns by considering the simultaneous internal and external states of the rotary machine. Extracting internal and external patterns, each pattern is combined for identifying convergence patterns, named MCP. Each MCP proceeds labeling process, named MCPL, then MCPL dataset is structured. MCPL dataset is verified based on Deep Neural Network (DNN) and Convolutional Neural Network (CNN). By utilizing the DNN and CNN techniques to derive MCPL from MCP, it becomes possible to perform unbiased state diagnosis across a variety of patterns, based on the complex patterns of the internal and external states of the rotating machinery. Presenting high accuracy and stable results, MCPL are able to classify rotary machine states and detect anomalies under the convergence environment. Our source code and utilized data are available onhttps://github.com/JEJESBSB/Journal-of-Big-Data.