bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stratigraphic evidence for culturally variable Indigenous fire regimes in ponderosa pine forests of the Mogollon Rim area, east-central Arizona
Authors: Roos Christopher I. , Laluk Nicholas C. , Reitze William , Davis Owen K. 
Published: December 2022

AbstractThe impact of Indigenous populations on historical fire regimes has been controversial and beset by mismatches in the geographic scale of paleofire reconstructions and the scale of land-use behaviors. It is often assumed that anthropogenic burning is linearly related to population density and not different cultural practices. Here we take an off-site geoarchaeology strategy to reconstruct variability in historical fire regimes (<1000 years ago) at geographic scales that match the archaeological, ethnohistorical, and oral tradition evidence for variability in the intensity of Indigenous land use by two different cultural groups (Ancestral Pueblo and Western Apache). We use multiple, independent proxies from three localities in ponderosa pine (Pinus ponderosa) forests in east-Central Arizona to reconstruct fire regime variability during four phases of cultural use of different intensities. Elevated charcoal with domesticate pollen (Zeaspp.) but otherwise unchanged forest pollen assemblages characterized intensive land use by Ancestral Pueblo people during an early phase, suggesting fire use to support agricultural activities. By contrast, a phase of intensive pre-reservation Western Apache land use corresponded to little change in charcoal, but had elevated ash-derived phosphorus and elevated grass and ruderal pollen suggestive of enhanced burning in fine fuels to promote economically important wild plants.
Commercial television and primate ethology: facial expressions between Granada and London Zoo
Authors: Kempton Miles 
Published: December 2022

AbstractThis article examines the significant relationship that existed between commercial British television and the study of animal behaviour. Ethological research provided important content for the new television channel, at the same time as that coverage played a substantial role in creating a new research specialism, the study of primate facial expressions, for this emergent scientific discipline. The key site in this was a television and film unit at London Zoo administered by the Zoological Society and Granada TV. The Granada unit produced ‘Animal expressions’, a twenty-five-minute television film based on research on monkeys and apes by the Dutch postgraduate student and soon-to-be-leading-authority Jan van Hooff. Recovering the production and multiple uses of ‘Animal expressions’, this paper offers the first sustained historical analysis of science on commercial television. I show how Granada patronage helped Van Hooff to support his argument that human expressions such as smiling and laughter shared common evolutionary origins with similar facial movements in nonhuman primates. Emphasizing the mutual shaping of science and ITV, I argue that ‘Animal expressions’ repurposed televisual conventions of framing talking heads, and blended serious science with the comedy of ‘funny faces’, thereby epitomizing Granada's public-service strategy at a time when commercial television was defending itself from criticism in the Pilkington report.
Does Pten have an impact on oogenesis of PCOS mouse models?
Authors: Onal T. , Tulay P. , Vatansever H.S. 
Published: December 2022

SummaryPolycystic ovary syndrome (PCOS) is a complex disorder in which the aetiology is still not explained very well. The PI3K/PTEN (phosphatidylinositol 3-kinase/phosphatase and tensin homolog deleted on chromosome 10) pathway is an important pathway that is involved in many mechanisms, including proliferation, growth and motility. PTEN plays a role in granulosa cell proliferation and regulates the differentiation process. The aim of this study was to investigate the expression levels of Pten and Pik3ca in PCOS mouse models with and without any treatment procedures. Three groups of mouse models, PCOS, a PCOS group with clomiphene citrate treatment, and a PCOS group with the combination of clomiphene citrate, metformin and pioglitazone treatment, were established. Ovarian tissues, which were obtained from these groups and a control group with no PCOS, were embedded in paraffin and RNA was extracted. cDNA was synthesized and real-time PCR was conducted to evaluate the expression levels of Pten and Pik3ca. The results of this study showed that both Pten and Pik3ca genes were expressed in the ovarian tissues from the mouse models. Although one-way analysis of variance results showed that Pten was expressed significantly differently in the samples, individual Student’s t-tests did not show any significantly different expression levels in each group. This study is important as it shows the expression patterns of two genes in PCOS mouse models with different treatment strategies, including clomiphene citrate, metformin and pioglitazone. The results of this study formed the basis of research studies and investigations into different genes within the PTEN pathway, as well as other pathways that are under investigation.
Late systemic desaturation after transcatheter device closure of atrial septal defect
Authors: Bagaria Vivek , Hiremath Channabasavaraj S. 
Published: December 2022

AbstractTranscatheter device closure has emerged as the preferred treatment for ostium secundum atrial septal defects with adequate margins, occasionally requiring surgical intervention to manage complications. We report a case of a young female patient who presented with systemic desaturation and exertional dyspnoea 5 years after device closure. Evaluation demonstrated anterior malposition of the septal occluder, with the right atrial rim of the device diverting the blood from the inferior vena cava to the left atrium. She underwent surgical extraction of the device and pericardial patch closure of the atrial septal defect.
Fall-sown small grain cover crops for weed suppression and soil moisture management in an irrigated organic agroecosystem
Authors: Pratt Richard C. , Schutte Brian J. , Idowu O. John , Uchanski Mark , Grant Lois 
Published: December 2022

Abstract
Adoption of cover crops in arid agroecosystems has been slow due to concerns regarding limited water resources and possible soil moisture depletion. In irrigated organic systems, potential ecosystem services from cover crops also must be considered in light of the concerns for water conservation. A constructive balance could be achieved with fall-sown small grain cover crops; however, their impacts on irrigated organic systems are poorly understood. Our first objective was to determine the ability of fall-sown small grains [cereal rye (Secale cereale L), winter wheat (Triticum aestivum L.), barley (Hordeum vulgare L.) and oat (Avena sativa L.)] to suppress winter weeds in an irrigated, organic transition field in the southwestern USA. Small grains were planted following the legume sesbania (Sesbania exaltata (Raf.) Rydb. ex A.W. Hill) during Fall 2012 and Fall 2013. In Spring 2013 and 2014, weed densities and biomass were determined within each cover crop treatment and compared against unplanted controls. Results indicated that both barley and oat were effective in suppressing winter weeds. Our second objective was to compare weed suppression and soil moisture levels among seven barley varieties developed in the western United States. Barley varieties (‘Arivat’, ‘Hayes Beardless’, ‘P919’, ‘Robust’, ‘UC603’, ‘UC937’, ‘Washford Beardless’) were fall-sown in replicated strip plots in Fall 2016. Weed densities were measured in Spring 2017 and volumetric soil moisture near the soil surface (5.1 cm depth) was measured at time intervals beginning in December 2016 and ending in March 2017. With the exception of ‘UC937’, barley varieties caused marked reductions in weed density in comparison with the unplanted control. Soil moisture content for the unplanted control was consistently lower than soil moisture contents for barley plots. Barley variety did not influence volumetric soil moisture. During the 2017–2018 growing season, we re-examined three barley varieties considered most amenable to the cropping system requirements (‘Robust’, ‘UC603’, ‘P919’), and these varieties were again found to support few weeds (≤ 5.0 weeds m−2). We conclude that several organically certified barley varieties could fill the need for a ‘non-thirsty’ cover crop that suppresses winter weeds in irrigated organic systems in the southwestern United States.
Basking in Their Glory? Expressive Partisanship among People of Color Before and After the 2020 US Election
Authors: Maxwell Rahsaan , Pérez Efrén , Zonszein Stephanie 
Published: December 2022

AbstractAs the number of people of color (PoC) grows in the United States, a key question is how partisanship will develop among this important electoral group. Yet many open questions remain about PoC partisanship, due to limited availability of panel data, a lack of sensitive instrumentation, and small samples of PoC in most public opinion surveys. This brief report leverages a unique panel of African American (N = 650) and Latino (N = 650) eligible voters, before and after the 2020 Presidential Election between Democrat Joe Biden and Republican Donald Trump. Using measures that tap expressive partisan, racial, and national identity attachments, we find that Biden’s electoral victory significantly intensified partisan identity among his Democratic PoC supporters, relative to PoC who were not Democrats and supported Trump. We do not find significant changes in racial or national identities. Our results advance research on PoC’s partisanship.
MAVEN/NGIMS Dayside Exospheric Temperatures Over Solar Cycle and Seasons: Role of Dayside Thermal Balances in Regulating Temperatures
Authors: Bougher S. W. , Benna M. , Elrod M. , Roeten K. , Thiemann E. 
Published: December 2022

AbstractSeveral important processes of the Mars upper atmosphere are regulated by exospheric temperature (Texo) variations, including atmospheric escape rates. From the MAVEN mission, significant and largely periodic variability of Mars dayside Texo is now revealed by Neutral Gas and Ion Mass Spectrometer (NGIMS) data sets collected throughout solar cycle 24. Two complementary methods are utilized to extract temperatures from NGIMS data sets. These NGIMS dayside mean exospheric temperatures are shown to vary by ∼80 K (∼180–260 K) over solar cycle 24. This corresponds to a ΔTexo/ΔEUV sensitivity of ∼38 K m2 mW−1, where Lyman‐α is the solar index. Previous Mars Global Surveyor derived Texo values for solar cycle 23 yielded a sensitivity of ∼45 in the same units. This close correspondence suggests that the underlying dayside energy balances are similar yet slightly different over these two solar cycles. Corresponding Mars Global Ionosphere‐Thermosphere Model simulations show that molecular thermal conduction largely balances EUV heating for the Mars dayside thermosphere, while CO2 15‐µm cooling is secondary in importance, along with global winds. It is valuable to compare this Mars sensitivity to that computed for the dayside thermosphere of Venus. Pioneer Venus dayside data sets imply a sensitivity of ∼17.5 K m2 mW−1 units for solar cycle 21, a factor of ∼2.2 smaller than computed for Mars. This is because Venus CO2 cooling strongly balances EUV heating near its peak, thereby serving as a more efficient thermostat regulating dayside temperatures. CO2 cooling is much weaker for Mars.
The Spatiotemporal Variability of Snowpack and Snowmelt Water 18O and 2H Isotopes in a Subarctic Catchment
Authors: Noor Kashif , Marttila Hannu , Klöve Björn , Welker Jeffrey M. , Ala‐aho Pertti 
Published: December 2022

AbstractThis study provides a detailed characterization of spatiotemporal variations of stable water 18O and 2H isotopes in both snowpack and meltwater in a subarctic catchment. We performed extensive sampling and analysis of snowpack and meltwater isotopic compositions at 11 locations in 2019 and 2020 across three different landscape features: (a) forest hillslope, (b) mixed forest, and (c) open mires. The vertical isotope profiles in the snowpack's layered stratigraphy presented a consistent pattern in all locations before snowmelt, and isotope profiles homogenized during the peak melt period; represented by a 1–2‰ higher 18O value than prior to melting. Our data indicated that the liquid‐ice fractionation was the prime reason that caused the depletion of heavy isotopes in initial meltwater samples prior to the peak melt period. The liquid‐ice fractionation was influenced by snowmelt rate, with higher fractionation during slow melt. The kinetic liquid‐ice fractionation was evident only in close examination of meltwater lc‐excess values, not 18O values alone. Meltwater was isotopically heavier and more variable than the depth‐integrated snowpack; the weighted mean of meltwater isotope values was higher by 0.62–1.33‰ 18O than the weighted mean of snowpack isotope values in forest hillslope and mixed forest areas, and 1.51–6.37‰ 18O in open mires. Our results reveal close to 3.1‰ 18O disparity between the meltwater and depth‐integrated snowpack isotope values prior to the peak melt period, suggesting that proper characterization of meltwater 18O and 2H values is vital for tracer‐based ecohydrological studies and models.
Endothelial cells regulate astrocyte to neural progenitor cell trans-differentiation in a mouse model of stroke
Authors: Li Wenlu , Mandeville Emiri T. , Durán-Laforet Violeta , Fukuda Norito , Yu Zhanyang , Zheng Yi , Held Aaron , Park Ji-Hyun , Nakano Takafumi , Tanaka Masayoshi , Shi Jingfei , Esposito Elga , Niu Wanting , Xing Changhong , Hayakawa Kazuhide , Lizasoain Ignacio , van Leyen Klaus , Ji Xunming , Wainger Brian J. , Moro Maria A. , Lo Eng H. 
Published: December 2022

AbstractThe concept of the neurovascular unit emphasizes the importance of cell-cell signaling between neural, glial, and vascular compartments. In neurogenesis, for example, brain endothelial cells play a key role by supplying trophic support to neural progenitors. Here, we describe a surprising phenomenon where brain endothelial cells may release trans-differentiation signals that convert astrocytes into neural progenitor cells in male mice after stroke. After oxygen-glucose deprivation, brain endothelial cells release microvesicles containing pro-neural factor Ascl1 that enter into astrocytes to induce their trans-differentiation into neural progenitors. In mouse models of focal cerebral ischemia, Ascl1 is upregulated in endothelium prior to astrocytic conversion into neural progenitor cells. Injecting brain endothelial-derived microvesicles amplifies the process of astrocyte trans-differentiation. Endothelial-specific overexpression of Ascl1 increases the local conversion of astrocytes into neural progenitors and improves behavioral recovery. Our findings describe an unexpected vascular-regulated mechanism of neuroplasticity that may open up therapeutic opportunities for improving outcomes after stroke.
The tongue biofilm metatranscriptome identifies metabolic pathways associated with the presence or absence of halitosis
Authors: Carda-Diéguez M. , Rosier B.T. , Lloret S. , Llena C. , Mira A. 
Published: December 2022

AbstractIntra-oral halitosis usually results from the production of volatile sulfur compounds, such as methyl mercaptan and hydrogen sulfide, by the tongue microbiota. There are currently no reports on the microbial gene-expression profiles of the tongue microbiota in halitosis. In this study, we performed RNAseq of tongue coating samples from individuals with and without halitosis. The activity of Streptococcus (including S. parasanguinis), Veillonella (including V. dispar) and Rothia (including R. mucilaginosa) was associated with halitosis-free individuals while Prevotella (including P. shahi), Fusobacterium (including F. nucleatum) and Leptotrichia were associated with halitosis. Interestingly, the metatranscriptome of patients that only had halitosis levels of methyl mercaptan was similar to that of halitosis-free individuals. Finally, gene expression profiles showed a significant over-expression of genes involved in L-cysteine and L-homocysteine synthesis, as well as nitrate reduction genes, in halitosis-free individuals and an over-expression of genes responsible for cysteine degradation into hydrogen sulfide in halitosis patients.
A phase I clinical study to assess safety and immunogenicity of yellow fever vaccine
Authors: Desai Sajjad , Anil K. , Potey Anirudha Vyankatesh , Sindhu Y. , Grappi Silvia , Lapini Giulia , Manney Satyaprasad , Tyagi Parikshit , Montomoli Emanuele , Poonawalla Cyrus S. , Kulkarni Prasad S. 
Published: December 2022

AbstractYellow fever, a mosquito-borne flavivirus infection, is an important public health problem in Africa and Latin America. A Yellow Fever vaccine (YFV) was developed and tested in a study in India. This was a Phase I, open-label, randomized, controlled study where healthy adults received SII YFV intramuscularly (SII YFV IM), SII YFV subcutaneously (SII YFV SC) or STAMARIL® (Sanofi-Pasteur) in 1:1:1 ratio. They were followed for solicited reactions for 10 days and unsolicited events for 28 days and serious adverse events for 3 months. YF-neutralizing antibodies were measured at baseline and on Days 10, 14, 28. A total of 60 adults were enrolled in the study. The proportion of participants with solicited reactions was 10%, 40%, and 25% in SII YFV SC, SII YFV IM, and STAMARIL® arms, respectively. No causally related unsolicited events or any serious adverse event was reported. After vaccination, the seroconversion was 94.44%, 100%, and 100%, in the three arms respectively. The post-vaccination geometric mean titers were similar in the study arms. The new YFV was found safe and immunogenic by IM as well as SC routes. The vaccine can be tested in further phases of clinical studies.
Challenges and developments in universal vaccine design against SARS-CoV-2 variants
Authors: Zhao Fangxin , Zai Xiaodong , Zhang Zhiling , Xu Junjie , Chen Wei 
Published: December 2022

AbstractThe emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) had become a global concern because of its unexpectedly high pathogenicity and transmissibility. SARS-CoV-2 variants that reduce the immune protection elicited from previous vaccination or natural infection raise challenges in controlling the spread of the pandemic. The development of universal vaccines against these variants seems to be a practical solution to alleviate the physical and economic effects caused by this disease, but it is hard to achieve. In this review, we describe the high mutation rate of RNA viruses and dynamic molecular structures of SARS-CoV-2 variants in several major neutralizing epitopes, trying to answer the question of why universal vaccines are difficult to design. Understanding the biological basis of immune evasion is crucial for combating these obstacles. We then summarize several advancements worthy of further study, including heterologous prime-boost regimens, construction of chimeric immunogens, design of protein nanoparticle antigens, and utilization of conserved neutralizing epitopes. The fact that some immunogens can induce cross-reactive immune responses against heterologous coronaviruses provides hints for universal vaccine development. We hope this review can provide inspiration to current universal vaccine studies.
Epipelagic nitrous oxide production offsets carbon sequestration by the biological pump
Authors: Wan Xianhui S. , Sheng Hua-Xia , Dai Minhan , Casciotti Karen L. , Church Matthew J. , Zou Wenbin , Liu Li , Shen Hui , Zhou Kuanbo , Ward Bess B. , Kao Shuh-Ji 
Published: December 2022

AbstractThe removal of carbon dioxide from the atmosphere by the marine biological pump is a key regulator of Earth’s climate; however, the ocean also serves as a large source of nitrous oxide, a potent greenhouse gas and ozone-depleting substance. Although biological carbon sequestration and nitrous oxide production have been individually studied in the ocean, their combined impacts on net greenhouse forcing remain uncertain. Here we show that the magnitude of nitrous oxide production in the epipelagic zone of the subtropical ocean covaries with remineralization processes and thus acts antagonistically to weaken the radiative benefit of carbon removal by the marine biological pump. Carbon and nitrogen isotope tracer incubation experiments and nitrogen isotope natural abundance data indicate enhanced biological activity promotes nitrogen recycling, leading to substantial nitrous oxide production via both oxidative and reductive pathways. These shallow-water nitrous oxide sources account for nearly half of the air–sea flux and counteract 6–27% (median 9%) of the greenhouse warming mitigation achieved by carbon export via the biological pump.
Ultrafast serrodyne optical frequency translator
Authors: Balla Prannay , Tünnermann Henrik , Salman Sarper H. , Fan Mingqi , Alisauskas Skirmantas , Hartl Ingmar , Heyl Christoph M. 
Published: December 2022

AbstractThe serrodyne principle enables an electromagnetic signal to be frequency shifted by applying a linear phase ramp in the time domain. This phenomenon has been exploited to frequency shift signals in the radiofrequency, microwave and optical regions of the electromagnetic spectrum over ranges of up to a few gigahertz, for example, to analyse the Doppler shift of radiofrequency signals for noise suppression and frequency stabilization. Here we employ this principle to shift the centre frequency of high-power femtosecond laser pulses over a range of several terahertz with the help of a nonlinear multi-pass cell. We demonstrate our method experimentally by shifting the central wavelength of a state-of-the-art 75 W frequency comb laser from 1,030 nm to 1,060 nm and to 1,000 nm. Furthermore, we experimentally show that this wavelength-shifting technique supports coherence characteristics at the few hertz-level while improving the temporal pulse quality. The technique is generally applicable to wide parameter ranges and different laser systems, enabling efficient wavelength conversion of high-power lasers to spectral regions beyond the gain bandwidth of available laser platforms.
Profound phenotypic and epigenetic heterogeneity of the HIV-1-infected CD4+ T cell reservoir
Authors: Wu Vincent H. , Nordin Jayme M. L. , Nguyen Son , Joy Jaimy , Mampe Felicity , del Rio Estrada Perla M. , Torres-Ruiz Fernanda , González-Navarro Mauricio , Luna-Villalobos Yara Andrea , Ávila-Ríos Santiago , Reyes-Terán Gustavo , Tebas Pablo , Montaner Luis J. , Bar Katharine J. , Vella Laura A. , Betts Michael R. 
Published: December 2022

AbstractUnderstanding the complexity of the long-lived HIV reservoir during antiretroviral therapy (ART) remains a considerable impediment in research towards a cure for HIV. To address this, we developed a single-cell strategy to precisely define the unperturbed peripheral blood HIV-infected memory CD4+ T cell reservoir from ART-treated people living with HIV (ART-PLWH) via the presence of integrated accessible proviral DNA in concert with epigenetic and cell surface protein profiling. We identified profound reservoir heterogeneity within and between ART-PLWH, characterized by new and known surface markers within total and individual memory CD4+ T cell subsets. We further uncovered new epigenetic profiles and transcription factor motifs enriched in HIV-infected cells that suggest infected cells with accessible provirus, irrespective of reservoir distribution, are poised for reactivation during ART treatment. Together, our findings reveal the extensive inter- and intrapersonal cellular heterogeneity of the HIV reservoir, and establish an initial multiomic atlas to develop targeted reservoir elimination strategies.