bluuebunny/crossref_metadata_2025_split huggingface dataset:
Keratin‐positive fibrotic extraskeletal myxoid chondrosarcoma: a close mimic of myoepithelial tumour
Authors: Sugino Hirokazu , Iwata Shintaro , Satomi Kaishi , Mori Taisuke , Nobusawa Sumihito , Nagashima Toshiteru , Matsushita Yuko , Yatabe Yasushi , Ichimura Koichi , Kawai Akira , Yoshida Akihiko 
Published: February 2023

AimsExtraskeletal myxoid chondrosarcoma (EMC) is a rare form of adult sarcoma with distinct histology and NR4A3 gene fusion. Immunohistochemically, EMCs are variably positive for S100 protein and neuroendocrine markers. Unlike histologically similar soft‐tissue myoepithelial tumours, keratin expression is rare. Prompted by two recent EMC cases with diffuse keratin expression, we investigated the expression of epithelial markers in a molecularly confirmed cohort of EMC and identified two additional similar cases.Methods and resultsFour keratin‐positive EMCs occurred in one man and three women aged 46–59 years. All tumours displayed nonclassic histology with prominent stromal fibrosis, and keratin AE1/AE3 was expressed either diffusely (N = 2) or focally (N = 2). In one tumour, keratin expression was limited to the sclerotic area. All tumours coexpressed epithelial membrane antigen and two additionally expressed S100 protein or glial fibrillary acidic protein. All tumours harboured NR4A3 fusions, including TAF15::NR4A3 (N = 1) and EWSR1::NR4A3 (N = 3). Two cases were initially considered as most consistent with myoepithelial tumours based on widespread stromal fibrosis and keratin expression. DNA methylation analysis classified two tumours tested as EMCs.ConclusionsWe identified a small subset of EMCs characterised by keratin expression and prominent stromal fibrosis. This histological pattern must be recognised in the differential diagnosis of myoepithelial tumours because misclassification may lead to the erroneous prediction of tumour behaviour and may alter patient management. NR4A3 genetic analysis should be considered even in the face of keratin expression and prominent stromal fibrosis.
Comparison of clinicopathological characteristics, gene expression profiles, mutational analysis, and clinical outcomes of pure and mixed small‐cell carcinoma of the bladder
Authors: Parimi Vamsi , Choi Woonyoung , Feng Mingxiao , Fong Megan , Hoffman‐Censits Jean , Kates Max , Lombardo Kara A. , Comperat Eva , McConkey David J. , Hahn Noah M. , Esteves Rodrigo Salgado , Matoso Andres 
Published: February 2023

AimsSmall cell bladder carcinoma (SCBC) is a rare, divergent form of urothelial carcinoma (UC). We aimed to determine whether pure (n = 16) and mixed (SCBC and UC; n = 30) tumours differed in pathology, gene expression characteristics, genetic alterations, and clinical outcomes.Methods and resultsForty (87%) patients received first‐line chemotherapy. Twenty‐nine patients had no metastatic disease at diagnosis and underwent radical cystectomy. There were no differences in age, sex, race distribution, tumour size, stage at presentation, therapy response with pathological downstaging to ≤ypT1N0, or overall or progression‐free survival (PFS) between pure and mixed tumours. There was a longer PFS among downstaged chemotherapy‐responding tumours ≤ypT2N0M0 than among unresponsive tumours ≥ypT2 ≥ yN1M1 (P = 0.001). Patients who achieved pathological downstaging with neoadjuvant chemotherapy (n = 10) were stage cT2N0M0 at the time of diagnosis and were alive at the last follow‐up (median 37 months), while 46% of patients who failed to achieve pathological downstaging were alive at the last follow‐up (median 38 months; P = 0.008). RNA sequencing showed that the UC of mixed SCBC had similar neural expression signatures to pure SCBC. DNA sequencing revealed alterations in TERT (83%), P53 (56%), ARID1A (28%), RB1 (22%), and BRCA2 (11%). Immunohistochemistry for RB1 showed loss of expression in 18/19 (95%) patients, suggesting frequent pathway downregulation despite a low prevalence of RB1 mutation.ConclusionPatients with pure and mixed SCBC have similar outcomes and these outcomes are determined by the pathological stage at RC and are best among patients who have pathological downstaging after NAC.
The effectiveness of self‐management programme based on multilevel social resources utilization in diabetes mellitus patients: A randomized controlled study
Authors: Zhang Feifei , Fu Liyan , Wang Lei , Xing Lu , Liu Kun , Jiang Xiaolian 
Published: February 2023

AbstractAimsThis study aimed to test the effects of a novel diabetes self‐management education (DSME) programme focused on multilevel social resources utilization on improving self‐management behaviour, social resources utilization and haemoglobin Alc (HbA1c) in patients with type 2 diabetes mellitus (T2DM).MethodsA randomized controlled design was used. A total of 118 participants with T2DM from two communities were recruited through randomized sampling, and randomly assigned to the novel DSME group or the traditional DSME group. DSME focusing on multilevel social resources utilization was implemented in the novel DSME group. Data were collected at baseline (T0), at the end of the intervention (T1) and 3 months after the end of intervention (T2) from 3 March to 16 November 2014.ResultsComparing these two groups, the participants in the novel DSME group reported significantly better improvement in self‐management behaviour and social resources utilization at T1 and T2 (all p < 0.01). A significant difference was found in the mean improvement of HbA1c level between the two groups at T2 (p < 0.05).ConclusionsThe novel DSME programme focused on multilevel social resources utilization can effectively enhance T2DM patients' self‐management behaviour and social resource utilization and lower HbA1c levels. More importantly, the effects could last longer.
Mothers' use of touch across infants' development and its implications for word learning: Evidence from Korean dyadic interactions
Authors: Ko Eon‐Suk , Abu‐Zhaya Rana , Kim Eun‐Sol , Kim Taehyeong , On Kyung‐Woon , Kim Hyunji , Zhang Byoung‐Tak , Seidl Amanda 
Published: February 2023

AbstractCaregivers' touches that occur alongside words and utterances could aid in the detection of word/utterance boundaries and the mapping of word forms to word meanings. We examined changes in caregivers' use of touches with their speech directed to infants using a multimodal cross‐sectional corpus of 35 Korean mother‐child dyads across three age groups of infants (8, 14, and 27 months). We tested the hypothesis that caregivers' frequency and use of touches with speech change with infants' development. Results revealed that the frequency of word/utterance‐touch alignment as well as word + touch co‐occurrence is highest in speech addressed to the youngest group of infants. Thus, this study provides support for the hypothesis that caregivers' use of touch during dyadic interactions is sensitive to infants' age in a way similar to caregivers' use of speech alone and could provide cues useful to infants' language learning at critical points in early development.
Improving sleep health management in primary care: A potential role for community nurses?
Authors: Basheti Mariam M. , Bawa Zeeta , Grunstein Ronald , Grivell Nicole , Saini Bandana , Gordon Christopher J. 
Published: February 2023

AbstractAimsTo explore community nurses sleep health practices and their perspectives on improving sleep health care provision.DesignAn exploratory study utilizing the qualitative description methodology.MethodsSemi‐structured interviews were conducted with community nurses from May 2019 – October 2021. Interviews were audio‐recorded, transcribed, and subjected to an inductive thematic analysis using a constructivist–interpretive paradigm.ResultsTwenty‐three Australian community nurses were interviewed. Participants frequently encountered sleep disturbances/disorders in their patients. Data analysis yielded three main themes: (1) Sleep health in the community serviced, (2) sleep health awareness and management, and (3) community nurses' A to Z of improving sleep health. The most common sleep disorder presentations were insomnia and sleep apnea. Although most community sleep apnea cases were appropriately managed, insomnia was often mismanaged. Participants described their sleep health knowledge as deficient, with the majority advocating for increased sleep‐related education tailored to their profession. Other important factors needed for improving sleep health provision were standardized patient treatment/referral pathways, increased interprofessional collaboration, and sufficient time for patient consults.ConclusionCommunity nurses service a patient population that requires increased sleep health care. However, they are currently underequipped to do so, leading to suboptimal treatment provision. Providing community nurses with the appropriate resources, such as increased sleep‐related education and standardized treatment frameworks, could enable them to better manage sleep disturbance/disorder presentations, such as insomnia.ImpactLittle is known about how community nurses care for patients with sleep disturbance/sleep disorders. This study found that contemporary sleep health care was lacking due to knowledge deficits, competing challenges, and a need for standardized care pathways. These findings can inform the development of targeted education/training and standardized guidelines for community nurses providing sleep health care to patients as well as the design of future practice models of care provision.Patient or Public ContributionPrevious research by authors has involved extensive engagement with patients and health professionals, such as community pharmacists, general practitioners, and naturopaths who play a role in sleep health in the primary health care sector. These previous research projects built a significant understanding of the patient and health practitioner experience and have provided the background to the concept and design of this study.
Blood pressure lowering effects of β‐blockers as add‐on or combination therapy: A meta‐analysis of randomized controlled trials
Authors: Guo Qian‐Hui , Zhu Zhi‐Ming , Feng Ying‐Qing , Lin Jin‐Xiu , Wang Ji‐Guang 
Published: February 2023

AbstractThe authors performed a meta‐analysis to assess the efficacy of non‐atenolol β‐blockers as add‐on to monotherapy or as a component of combination antihypertensive therapy in patients with hypertension. The authors searched and identified relevant randomized controlled trials from PubMed until November 2021. Studies comparing blood pressure lowering effects of β‐blockers with diuretics, calcium channel blockers (CCBs), angiotensin‐converting enzyme inhibitors (ACEIs), or angiotensin receptor blockers (ARBs) were included. The analysis included 20 studies with 5544 participants. β‐blockers add‐on to monotherapy significantly reduced systolic and diastolic blood pressure as compared with non‐β‐blocker monotherapy (weighted mean difference in mm Hg [95% confidence interval]: −4.1 [−6.0, −2.2] and −3.7 [−4.6, −2.8], respectively). These results were consistent across the comparisons with diuretics (systolic pressure, −10.2 [−14.2, −6.2]; diastolic pressure, −5.4 [−8.2, −2.6]), CCBs (systolic pressure, −4.1 [−7.1, −1.0]; diastolic pressure, −2.8 [−4.1, −1.5]), and ACEIs/ARBs (systolic pressure, −2.9 [−4.3, −1.5]; diastolic pressure, −4.2 [−5.0, −3.4]). There was no significant difference in blood pressure lowering effects between combinations with and without a β‐blocker (systolic pressure, −1.3 mm Hg [−5.8, 3.2]; diastolic pressure, −.3 mm Hg [−2.7, 2.1]). Metoprolol add‐on or combination therapy had a significantly greater blood pressure reduction than non‐β‐blocker therapy (systolic pressure, −3.6 mm Hg [−5.9, −1.3]; diastolic pressure, −2.1 mm Hg [−3.5, −.7]). In conclusion, non‐atenolol β‐blockers are effective in lowering blood pressure as add‐on to monotherapy or as a component of combination antihypertensive therapy. In line with the current hypertension guideline recommendations, β‐blockers can and should be used in combination with other antihypertensive drugs.
Hsa‐miR‐409‐3p regulates endothelial progenitor senescence via PP2A‐P38 and is a potential ageing marker in humans
Authors: Lee Yi‐Nan , Wu Yih‐Jer , Lee Hsin‐I , Wang Hsueh‐Hsiao , Hung Chung‐Lieh , Chang Chiung‐Yin , Chou Yen‐Hung , Tien Ting‐Yi , Lee Chun‐Wei , Lin Chao‐Feng , Su Cheng‐Huang , Yeh Hung‐I 
Published: February 2023

AbstractWe explored the roles of hsa‐microRNA (miR)‐409‐3p in senescence and signalling mechanism of human endothelial progenitor cells (EPCs). Hsa‐miR‐409‐3p was found upregulated in senescent EPCs. Overexpression of miRNA mimics in young EPCs inhibited angiogenesis. In senescent EPCs, compared to young EPCs, protein phosphatase 2A (PP2A) was downregulated, with activation of p38/JNK by phosphorylation. Young EPCs treated with siPP2A caused inhibited angiogenesis with activation of p38/JNK, similar to findings in senescent EPCs. Time series analysis showed, in young EPCs treated with hsa‐miR‐409‐3p mimics, PP2A was steadily downregulated for 72 h, while p38/JNK was activated with a peak at 48 hours. The inhibited angiogenesis of young EPCs after miRNA‐409‐3p mimics treatment was reversed by the p38 inhibitor. The effect of hsa‐miR‐409‐3p on PP2A signalling was attenuated by exogenous VEGF. Analysis of human peripheral blood mononuclear cells (PBMCs) obtained from healthy people revealed hsa‐miR‐409‐3p expression was higher in those older than 65 years, compared to those younger than 30 years, regardless of gender. In summary, hsa‐miR‐409‐3p was upregulated in senescent EPCs and acted as a negative modulator of angiogenesis via targeting protein phosphatase 2 catalytic subunit alpha (PPP2CA) gene and regulating PP2A/p38 signalling. Data from human PBMCs suggested hsa‐miR‐409‐3p a potential biomarker for human ageing.
Estimating the risk reduction of isolation on COVID‐19 nonhousehold transmission and severe/critical illness in nonimmune individuals: September to November 2021
Authors: Prosser Aaron , Helfer Bartosz , Streiner David L. 
Published: February 2023

AbstractThere is growing scientific interest in immunity mandates/passports (IMP) for viral diseases in light of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic. IMP isolate those who remain nonimmune from various settings to reduce nonhousehold transmissions from the nonimmune and reduce severe/critical illness among the nonimmune. A major limitation in the scientific literature is that there are currently no methods to quantify how many nonimmune individuals need to be isolated to achieve these purported benefits. This paper develops a procedure for estimating the benefits of IMP using a novel variant of the number needed to treat which we call the number needed to isolate (NNI). We use data from the SARS‐CoV‐2 pandemic to demonstrate the properties and utility of the NNI and to inform the debate about IMP. We focus on data from the European Union, United Kingdom, United States, Canada, Australia, and Israel during the fall 2021 when the Delta (B.1.617.2) variant predominated.
Controlled atmosphere maintains the quality of certain fresh produce in mixed cargo shipments
Authors: Ikegaya Atsushi , Yamazaki Shigehiro , Yamaga Ittetsu , Kosugi Toru , Toyoizumi Tomoyasu , Nagafuji Akihiko , Saika Takehiro , Arai Eiko 
Published: February 2023

AbstractShipping containers are used globally for the domestic distribution and export of several products. In this study, we compared the efficacy of controlled atmosphere (CA) containers to reefer containers based on the quality of 41 fresh fruits and vegetables exported from Japan to Singapore. We used instrumental analysis and sensory evaluation to compare the quality of fruits and vegetables transported in these two container types. Thirteen types of produce showed higher quality in the CA container than in the reefer container. Two types of fruits deteriorated in flavor due to abnormal maturation under CA conditions. Six types of produce showed higher quality in the reefer containers than in the CA containers. The CA containers were particularly effective in improving the quality of leafy vegetables, root vegetables, and fresh legumes; thus, CA containers are excellent for exporting these types of produce.Practical applicationsThe export of fresh fruits and vegetables by CA containers is expanding year after year, but their operation is difficult because the optimal storage environment for fruits and vegetables varies from item to item. This study showed the effectiveness of CA containers in improving the quality of leafy vegetables, root vegetables, and fresh legumes during their export from Japan to Singapore. These findings would contribute to the expansion of exports using CA containers in the future.
Prediction models for the impact of the COVID‐19 pandemic on research activities of Japanese nursing researchers using deep learning
Authors: Lee Kumsun , Takahashi Fusako , Kawasaki Yuki , Yoshinaga Naoki , Sakai Hiroko 
Published: February 2023

AbstractAimThis study aimed to construct and evaluate prediction models using deep learning to explore the impact of attributes and lifestyle factors on research activities of nursing researchers during the COVID‐19 pandemic.MethodsA secondary data analysis was conducted from a cross‐sectional online survey by the Japanese Society of Nursing Science at the inception of the COVID‐19 pandemic. A total of 1089 respondents from nursing faculties were divided into a training dataset and a test dataset. We constructed two prediction models with the training dataset using artificial intelligence (AI) predictive analysis tools; motivation and time were used as predictor items for negative impact on research activities. Predictive factors were attributes, lifestyle, and predictor items for each other. The models' accuracy and internal validity were evaluated using an ordinal logistic regression analysis to assess goodness‐of‐fit; the test dataset was used to assess external validity. Predicted contributions by each factor were also calculated.ResultsThe models' accuracy and goodness‐of‐fit were good. The prediction contribution analysis showed that no increase in research motivation and lack of increase in research time strongly influenced each other. Other factors that negatively influenced research motivation and research time were residing outside the special alert area and lecturer position and living with partner/spouse and associate professor position, respectively.ConclusionsDeep learning is a research method enabling early prediction of unexpected events, suggesting new applicability in nursing science. To continue research activities during the COVID‐19 pandemic and future contingencies, the research environment needs to be improved, workload corrected by position, and considered in terms of work‐life balance.
Hematological parameters in patients with acnes
Authors: Chen Tingqiao , Chen Yangmei , Shao Xinyi , Chen Jiayi , Liu Lin , Li Yuxin , Pu Yihuan , Chen Jin 
Published: February 2023

AbstractObjectiveTo compare complete blood count (CBC) parameters and inflammatory factors in the patients with different grade of acne vulgaris and healthy controls.MethodsA total of 20 patients were enrolled in this study. Patients were divided into mild group and moderate‐to‐severe group based on the acne severity, and compared to controls. Inflammatory factors (TNF‐α, IL‐6, IL‐8, and IL1‐α) detected by ELISA and complete blood count parameters (MPV, NLR, dNLR, PLR, LMR, and SII) obtained by routine blood tests were compared among the three group.ResultsAll CBC parameters were not significantly elevated in patients with acne compared to healthy controls. However, the present studies have found that the inflammatory factors in acne patients were significantly elevated relative to healthy controls, and increase with the acne grade.ConclusionsInflammatory factors are convenient parameters to show inflammatory response to acne vulgaris, and may be a new clinical method for judging the acne grades of objectively. Considering the use of antibiotic, we believe that this metric worth further study.
Winchcombe: An example of rapid terrestrial alteration of a CM chondrite
Authors: Jenkins Laura E. , Lee Martin R. , Daly Luke , King Ashley J. , Floyd Cameron J. , Martin Pierre‐Etienne , Almeida Natasha V. , Genge Matthew J. 
Published: February 2023

AbstractWinchcombe is a CM chondrite that fell in England on February 28, 2021. Its rapid retrieval was well characterized. Within two polished sections of Winchcombe, terrestrial phases were observed. Calcite and calcium sulfates were found in a sample recovered from a field on March 6, 2021, and halite was observed on a sample months after its recovery from a driveway on March 2, 2021. These terrestrial phases were characterized by scanning electron microscopy, Raman spectroscopy, and transmission electron microscopy. Calcite veins crosscut the fusion crust and therefore postdate it. The calcite likely precipitated in the damp environment (sheep field) where the meteorite lay for six days prior to its retrieval. The sulfates occur on the edges of the sample and were identified as three minerals: gypsum, bassanite, and anhydrite. Given that the sulfates occur only on the sample's edges, including on top of the fusion crust, they formed after Winchcombe fell. Sulfate precipitation is attributed to the damp fall environment, likely resulted from sulfide‐derived H2S reacting with calcite within the meteorite. Halite occurs as euhedral crystals only on the surface of a polished section and exclusively in areas relatively enriched in sodium. It was likely produced by the interaction of the polished rock slice with the humid laboratory air over a period of months. The sulfates, fusion crust calcite, and halite all post‐date Winchcombe's entry into the Earth's atmosphere and showcase how rapidly meteorite falls can be terrestrially altered.
Excess copper catalyzes protein disulfide bond formation in the bacterial periplasm but not in the cytoplasm
Authors: Eben Stefanie S. , Imlay James A. 
Published: February 2023

AbstractCopper avidly binds thiols and is redox active, and it follows that one element of copper toxicity may be the generation of undesirable disulfide bonds in proteins. In the present study, copper oxidized the model thiol N‐acetylcysteine in vitro. Alkaline phosphatase (AP) requires disulfide bonds for activity, and copper activated reduced AP both in vitro and when it was expressed in the periplasm of mutants lacking their native disulfide‐generating system. However, AP was not activated when it was expressed in the cytoplasm of copper‐overloaded cells. Similarly, this copper stress failed to activate OxyR, a transcription factor that responds to the creation of a disulfide bond. The elimination of cellular disulfide‐reducing systems did not change these results. Nevertheless, in these cells, the cytoplasmic copper concentration was high enough to impair growth and completely inactivate enzymes with solvent‐exposed [4Fe‐4S] clusters. Experiments with N‐acetylcysteine determined that the efficiency of thiol oxidation is limited by the sluggish pace at which oxygen regenerates copper(II) through oxidation of the thiyl radical–Cu(I) complex. We conclude that this slow step makes copper too inefficient a catalyst to create disulfide stress in the thiol‐rich cytoplasm, but it can still impact the few thiol‐containing proteins in the periplasm. It also ensures that copper accumulates intracellularly in the Cu(I) valence.