bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison between percutaneous transthoracic co-axial needle CT-guided biopsy and transbronchial lung biopsy for the diagnosis of persistent pulmonary consolidation
Authors: Wang Juan , Zhang Tongyin , Xu Yanyan , Yang Meng , Huang Zhenguo , Lin Jie , Xie Sheng , Sun Hongliang 
Published: May 2023

Abstract
Background
Diagnosing persistent pulmonary consolidation still faces challenges. The purpose of this study is to compare the diagnostic yield and the complication rate between percutaneous transthoracic CT-guided coaxial needle biopsy (PTCNB) and transbronchial lung biopsy (TBLB) of persistent pulmonary consolidation.

Materials
From January 1, 2016, to December 31, 2020, we have retrospectively enrolled a total of 155 consecutive patients (95 males, 60 females) with persistent pulmonary consolidation who underwent both TBLB and PTCNB. According to the standard reference, the diagnostic yield, accuracy, sensitivity and specificity of PTCNB and TBLB were assessed and compared.

Results
According to the standard reference, the final biopsy diagnoses of 11 cases were confirmed true malignant based on the surgical resections, the remaining were confirmed by clinical and imaging follow-up for at least 12 months. The overall diagnostic accuracy, sensitivity and specificity of PTCNB for malignant diagnosis were 91.61%, 72.34% and 100%, whereas of TBLB were 87.74%, 59.57% and 100%. The diagnostic yield of PTCNB and TBLB were 50.32% and 25.16%, respectively. For the TBLB-based negative cases, PTCNB provided a definite diagnostic yield of 37.93%. There were 45 (29.03%), 22 (14.19%) and 13 (8.39%) patients who experienced pneumothorax, intrapulmonary hemorrhage and hemoptysis, respectively, in PTCNB, while there were only 5 (3.22%) cases of mild intraprocedural bleeding occurring in TBLB.

Conclusions
CT-guided co-axial needle biopsy is an effective and safe modality, associated with higher diagnostic yield and better diagnostic accuracy compared to transbronchial lung biopsy for malignancy presenting as persistent consolidation, especially as the complementary method for TBLB-based negative lung lesions.

Key Points

Both PTCNB and TBLB showed high diagnostic accuracy for malignancy.

PTCNB had a higher diagnostic yield than TBLB for persistent pulmonary consolidation.

PTCNB could provide a complementary diagnosis for TBLB-based negative lung consolidation.
Fermentation characteristics and radical scavenging capacities of ginseng berry kombucha fermented by Saccharomyces cerevisiae and Gluconobacter oxydans
Authors: Choi Eun Jung , Song Hee Ho , Ko Ka Young , Hong Ki-Bae , Suh Hyung Joo , Ahn Yejin 
Published: May 2023

AbstractKombucha is a healthy carbonated beverage made by fermenting tea extracts such as green tea and black tea through symbiotic culture of bacteria and yeast. In this study, fermentation characteristics and radical scavenging activity of ginseng berry kombucha (GBK) by Saccharomyces cerevisiae M-5 and Gluconobacter oxydans were measured. As fermentation time increased, pH decreased and titratable acidity increased. Reducing sugars decreased rapidly on day 3. Alcohol content increased dramatically during this period and then decreased. GBK showed increased radical scavenging activity and increased total flavonoid content on day 18 of fermentation compared to before fermentation. In particular, during GBK fermentation, the content of phenolic compounds such as gallic acid (2.09-fold) and chlorogenic acid (2.11-fold) increased, contributing to antioxidant activity. In addition, the major ginsenosides of GBK were identified as Rg2 (10.1 μg/mg) and Re (6.59 μg/mg), and the content of minor ginsenosides, which are easily absorbed forms, increased 2.19-fold by fermentation. GBK also extended survival in a Drosophila model treated with 15% hydrogen peroxide. GBK also reduced reactive oxygen species (p < 0.001) through upregulation of gene expression of antioxidant enzymes such as catalase (p < 0.001), superoxide dismutase (p < 0.05), and glutathione peroxidase (p < 0.001). Therefore, GBK can be presented as a functional food that inhibits oxidative stress by increasing radical scavenging activity during fermentation.
Gender representation in leadership & research: A 13-year review of the Annual Canadian Society of Otolaryngology Meetings
Authors: Yi Grace , Payandeh Jennifer , Mavedatnia Dorsa , Neocleous Penelope , Davidson Jacob , Siu Jennifer , Zirkle Molly , Strychowsky Julie E. , Graham M. Elise , Chan Yvonne 
Published: May 2023

Background The gender disparity in surgical disciplines, specifically in speakers across North American medical and surgical specialty conferences, has been highlighted in recent literature. Improving gender diversity at society meetings and panels may provide many benefits. Our aim was to determine the state of gender diversity amongst presenters and speakers at the annual Canadian Society of Otolaryngology-Head and Neck Surgery (CSO) meetings. Methods Scientific programs for the CSO annual meetings from 2008 to 2020 were obtained from the national society website. Participant name, role, gender, location, and subspecialty topic were recorded for all roles other than poster presenter. Gender (male or female) was determined using an online search. The total number of opportunity spots and proportion of women was then calculated. Gender differences were analyzed using chi-square test and logistic regression with odds ratios. Four categories were analyzed: Society Leadership, Invited Speaker Opportunities, Workshop Composition (male-only panels or “manels”, female-only panels, or with at least one female speaker), and Oral Paper Presenters (first authors). Results There were 1874 leadership opportunity spots from 2008 to 2020, of which 18.6% were filled by women. Among elected leadership positions in the society, only 92 unique women filled 738 leadership opportunity spots. 13.2% of workshop chairs, 20.8% of panelists and 22.7% of paper session chairs were female. There was an overall increase in the proportion of leadership positions held by women, from 13.9% of leadership spots in 2008 to 30.1% in 2020. Of the 368 workshops, 61.1% were led by men only, 36.4% by at least 1 female surgeon, and 2.5% by women only. “Manels” have comprised at least 37.5% of workshops each year. Conclusions The proportion of women in speaking roles at the annual CSO meetings has generally increased over time, particularly among panelists, leading to fewer male-only speaking panels. However, there has been a slower rate of growth in the proportion of unique women in speaker roles. There remains an opportunity to increase gender/sex diversity at the major Canadian otolaryngology meeting. Graphical Abstract
Could vertigo be a post-COVID-19 sequela or presenting symptom?
Authors: Daker Lamiaa I. , Elshafei Reham R. , Bahi Mohammad , Mohammed Asmaa , Erfan Randa , Gomaa Mohammed 
Published: May 2023

Abstract
Background
It has been postulated that COVID-19 has a substantial neuro-otological impact such as vertigo or dizziness that is rarely evaluated. The purpose of this research is to study the occurrence of vertigo (whether as presenting symptom or a sequela) and its etiological characteristics in patients with covid 19 infection and close contact. It is a cross-sectional study (convenient sample) conducted on patients that had a previous history of covid 19 infection and another group of contact individuals who presented with the sensation of vertigo. All the included participants underwent full neurological and otological examination, nasopharyngeal swab PCR to confirm COVID-19 infection and video nystgmograghy (VNG).

Results
it was included 44 participants, where 7 (15.9%) of the participants were post-COVID-19 patients and 37 (84.1%) were close contacts of COVID patients. It was found that 6(85.7%) of post-COVID-19 patients had vestibular neuritis (VN), and 1(14.3%) patient had Benign Paroxysmal Positional Vertigo (BPPV). 9(23%) of those in close contact had positive PCR for COVID infection, 6(66.7%) of them had VN, and the other 3 (33.3%) had BPPV.

Conclusion
Vertigo could be a possible complication or a presenting symptom in patients with COVID patients that is mainly attributed to peripheral vestibular dysfunction.
Interface design for residential energy feedback, in the Indian context
Authors: Garg Madhur , Garg Vishal , Srivastava Priyanka , Agarwal Rishika 
Published: May 2023

AbstractGlobal access to electricity has increased from 78.2% to 2000 to 90.5% in 2020, resulting in an increased electricity demand worldwide. Unlike commercial electricity consumption, which is managed by professionals, residential consumption is managed by the householders, who often lack insight into their energy usage. Quality feedback, including detailed energy consumption and tips, can lead to substantial household savings. There are several mediums for providing energy feedback, such as Short Message Service (SMS), postal letter, email, mobile app, and In-Home Display (IHD). Studies suggest that feedback through electronic media can save up to 20% of energy consumption. In this work, we aim to design mobile application interfaces that can maximize energy savings through effective feedback. The level of savings realized is dependent on the user’s preferences and understanding of the information presented. User preferences are subjective of their profile (e.g., age, occupation, income) and the cultural context (e.g., country). The possibility of energy reduction is high when the provided information matches the user preferred information for feedback. Smart homes have recently been included as an annexure in India’s building energy code (Eco Niwas Samhita 2021), indicating a growing demand for quality energy feedback in India. However, there is a lack of research that addresses what feedback information is suitable for Indian users. We conducted two questionnaire-based surveys, one to understand users’ preferences for feedback information and another to validate the designed mobile application interface screens. The surveys were conducted on two age groups, young and middle-aged adults. A Chi-Square Test of Independence was performed to assess the relationship between the user’s preference for feedback information and their age group. Participants identified total energy consumption, appliance level disaggregated information, energy-saving tips, goals, and historical consumption comparisons as the top five information types. In contrast, normative comparison was the least preferred information. The follow-up design validations suggest that the interface should be customizable to accommodate the varying preferences of users. The current findings will help customize the energy feedback display UI design as per the Indian population.
Graphical Abstract
Real-World First-Line Use of Pertuzumab With Different Taxanes for Human Epidermal Growth Factor Receptor 2–Positive Metastatic Breast Cancer: A Comparative Effectiveness Study Using US Electronic Health Records
Authors: Polito Letizia , Shim Jinjoo , Hurvitz Sara A. , Dang Chau T. , Knott Adam , Du Toit Yolande , Restuccia Eleonora , Sanglier Thibaut , Swain Sandra M. 
Published: May 2023

PURPOSE On the basis of the results from CLEOPATRA, pertuzumab plus trastuzumab and chemotherapy is the first-line standard of care for human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer (MBC). However, discrepancies have been reported between clinical trial and real-world outcomes. We report real-world outcomes for patients with HER2-positive MBC treated with first-line pertuzumab plus trastuzumab and a taxane in routine clinical practice in the United States. METHODS A retrospective analysis was conducted using electronic health record-derived deidentified data from the Flatiron Health database. Patients were grouped according to the first taxane received (paclitaxel/nab-paclitaxel or docetaxel). Median real-world progression-free survival (rwPFS) and overall survival (rwOS) was estimated using Kaplan-Meier methodology. Subgroup analyses were conducted in patients treated with docetaxel who met CLEOPATRA's key eligibility criteria. RESULTS We included 1,065 patients; 313 patients received paclitaxel/nab-paclitaxel and 752 received docetaxel. Patients who received paclitaxel/nab-paclitaxel were older, had a worse Eastern Cooperative Oncology Group Performance Status, and had more recurrent metastatic disease compared with the docetaxel group. After adjustment for potential confounders, similar median rwPFS (inverse probability of treatment weighted average treatment effect for the treated [IPTW-ATT] hazard ratio [HR], 1.09; 95% CI, 0.9 to 1.3; P = .365) and rwOS (IPTW-ATT HR, 1.23; 95% CI, 0.96 to 1.58; P = .101) was observed between treatment groups. In the subgroup of CLEOPATRA-eligible patients, median rwPFS and rwOS were 16.9 months and 57.8 months, respectively. CONCLUSION There was no statistically significant difference in real-world outcomes between patients treated with paclitaxel/nab-paclitaxel and those treated with docetaxel. Selecting patients using key CLEOPATRA eligibility criteria resulted in rwPFS and rwOS similar to those observed in CLEOPATRA, highlighting the importance of ensuring similar patient populations when comparing clinical trial and real-world data.
Genetic Prediction of Lifetime Risk of Fracture
Authors: Ho-Le Thao P , Tran Thach S , Nguyen Huy G , Center Jacqueline R , Eisman John A , Nguyen Tuan V 
Published: May 2023

Abstract

Context
Fragility fracture is a significant public health problem because it is associated with increased mortality. We want to find out whether the risk of fracture can be predicted from the time of birth.

Objective
To examine the association between a polygenic risk score (PRS) and lifetime fracture risk.

Methods
This population-based prospective study involved 3515 community-dwelling individuals aged 60+ years who have been followed for up to 20 years. Femoral neck bone mineral density (BMD) was measured by dual-energy x-ray absorptiometry. A PRS was created by summing the weighted number of risk alleles for each single nucleotide polymorphism using BMD-associated coefficients. Fragility fractures were radiologically ascertained, whereas mortality was ascertained through a state registry. Residual lifetime risk of fracture (RLRF) was estimated by survival analysis.

Results
The mortality-adjusted RLRF for women and men was 36% (95% CI, 34%-39%) and 21% (18%-24%), respectively. Individuals with PRS &gt; 4.24 (median) had a greater risk (1.2-fold in women and 1.1-fold in men) than the population average risk. For hip fracture, the average RLRF was 10% (95% CI, 8%-12%) for women and ∼5% (3%-7%) for men; however, the risk was significantly increased by 1.5-fold and 1.3-fold for women and men with high PRS, respectively.

Conclusion
A genetic profiling of BMD-associated genetic variants is associated with the residual lifetime risk of fracture, suggesting the potential for incorporating the polygenic risk score in personalized fracture risk assessment.
Unexpected Plasma Gonadal Steroid and Prolactin Levels Across the Mouse Estrous Cycle
Authors: Wall Ellen G , Desai Reena , Khant Aung Zin , Yeo Shel Hwa , Grattan David R , Handelsman David J , Herbison Allan E 
Published: May 2023

AbstractDespite the importance of the mouse in biomedical research, the levels of circulating gonadal steroids across the estrous cycle are not established with any temporal precision. Using liquid chromatography–mass spectrometry, now considered the gold standard for steroid hormone analysis, we aimed to generate a detailed profile of gonadal steroid levels across the estrous cycle of C57BL/6J mice. For reference, luteinizing hormone (LH) and prolactin concentrations were measured in the same samples by sandwich enzyme-linked immunosorbent assay. Terminal blood samples were collected at 8-hour intervals (10 Am, 6 Pm, 2 Am) throughout the 4 stages of the estrous cycle. As expected, the LH surge was detected at 6 Pm on proestrus with a mean (±SEM) concentration of 11 ± 3 ng/mL and occurred coincident with the peak in progesterone levels (22 ± 4 ng/mL). Surprisingly, estradiol concentrations peaked at 10 Am on diestrus (51 ± 8 pg/mL), with levels on proestrus 6 Pm reaching only two-thirds of this value (31 ± 5 pg/mL). We also observed a proestrus peak in prolactin concentrations (132.5 ± 17 ng/mL) that occurred earlier than expected at 2 Am. Estrone and androstenedione levels were often close to the limit of detection (LOD) and showed no consistent changes across the estrous cycle. Testosterone levels were rarely above the LOD (0.01 ng/mL). These observations provide the first detailed assessment of fluctuating gonadal steroid and reproductive hormone levels across the mouse estrous cycle and indicate that species differences exist between mice and other spontaneously ovulating species.
Implementation of Nephroprotective Measures to Prevent Acute Kidney Injury in Septic Patients: A Retrospective Cohort Study
Authors: Sadjadi Mahan , Porschen Christian , von Groote Thilo , Albert Felix , Kellum John A. , Gomez Hernando , Meersch Melanie , Zarbock Alexander 
Published: May 2023

BACKGROUND:
Sepsis remains the leading cause of mortality in critically ill patients, and mortality is increased when acute kidney injury (AKI) occurs. The Kidney Disease: Improving Global Outcomes (KDIGO) guideline recommends the implementation of supportive measures in patients at high risk for AKI. However, it remains unclear to what extent these nephroprotective measures are implemented in daily clinical practice in critically ill patients, especially those with high-risk exposures such as sepsis.

METHODS:
We analyzed the Medical Information Mart for Intensive Care IV (MIMIC-IV) database to identify septic patients with and without AKI. The primary outcome of interest was the adherence to the KDIGO bundle consisting of avoidance of nephrotoxic agents, implementation of a functional hemodynamic monitoring, optimization of perfusion pressure and volume status, close monitoring of renal function, avoidance of hyperglycemia, and avoidance of radiocontrast agents. Secondary outcomes included the development of AKI, progression of AKI, the use of renal replacement therapy (RRT), mortality, and a composite end point consisting of progression of AKI and mortality within 7 days.

RESULTS:
Our analysis included 34,679 patients with sepsis with 1.6% receiving the complete bundle (10% received 5, 42.3% 4, 35.4% 3, and 9.8% 2 bundle components). In 56.4%, nephrotoxic agents were avoided, and hemodynamic optimization was reached in 86.5%. Secondary end points were improved in patients with bundle adherence. Avoidance of nephrotoxic drugs and optimization of hemodynamics were significantly associated with lower rates of AKI and improved patient outcomes, including 30-day mortality.

CONCLUSIONS:
Implementation of the KDIGO bundle is poor in patients with sepsis but may be associated with improved outcomes.
Distinct hyperactive RAS/MAPK alleles converge on common GABAergic interneuron core programs
Authors: Knowles Sara J. , Stafford April M. , Zaman Tariq , Angara Kartik , Williams Michael R. , Newbern Jason M. , Vogt Daniel 
Published: May 2023

ABSTRACT
RAS/MAPK gene dysfunction underlies various cancers and neurocognitive disorders. Although the roles of RAS/MAPK genes have been well studied in cancer, less is known about their function during neurodevelopment. There are many genes that work in concert to regulate RAS/MAPK signaling, suggesting that if common brain phenotypes could be discovered they could have a broad impact on the many other disorders caused by distinct RAS/MAPK genes. We assessed the cellular and molecular consequences of hyperactivating the RAS/MAPK pathway using two distinct genes in a cell type previously implicated in RAS/MAPK-mediated cognitive changes, cortical GABAergic interneurons. We uncovered some GABAergic core programs that are commonly altered in each of the mutants. Notably, hyperactive RAS/MAPK mutants bias developing cortical interneurons towards those that are somatostatin positive. The increase in somatostatin-positive interneurons could also be prevented by pharmacological inhibition of the core RAS/MAPK signaling pathway. Overall, these findings present new insights into how different RAS/MAPK mutations can converge on GABAergic interneurons, which may be important for other RAS/MAPK genes and related disorders.
Insights into the pathophysiology of DFNA44 hearing loss associated with CCDC50 frameshift variants
Authors: Lachgar-Ruiz María , Morín Matías , Martelletti Elisa , Ingham Neil J. , Preite Lorenzo , Lewis Morag A. , Serrão de Castro Luciana Santos , Steel Karen P. , Moreno-Pelayo Miguel Ángel 
Published: May 2023

ABSTRACT
Non-syndromic sensorineural hearing loss (SNHL) is the most common sensory disorder, and it presents a high genetic heterogeneity. As part of our clinical genetic studies, we ascertained a previously unreported mutation in CCDC50 [c.828_858del, p.(Asp276Glufs*40)] segregating with hearing impairment in a Spanish family with SNHL associated with the autosomal dominant deafness locus DFNA44, which is predicted to disrupt protein function. To gain insight into the mechanism behind DFNA44 mutations, we analysed two Ccdc50 presumed loss-of-function mouse mutants, which showed normal hearing thresholds up to 6 months of age, indicating that haploinsufficiency is unlikely to be the pathogenic mechanism. We then carried out in vitro studies on a set of artificial mutants and on the p.(Asp276Glufs*40) and p.(Phe292Hisfs*37) human mutations, and determined that only the mutants containing the six-amino-acid sequence CLENGL as part of their aberrant protein tail showed an abnormal distribution consisting of perinuclear aggregates of the CCDC50 protein (also known as Ymer). Therefore, we conclude that the CLENGL sequence is necessary to form these aggregates. Taken together, the in vivo and in vitro results obtained in this study suggest that the two identified mutations in CCDC50 exert their effect through a dominant-negative or gain-of-function mechanism rather than by haploinsufficiency.
MODERN EDUCATIONAL TECHNOLOGIES IN TEACHING A FOREIGN LANGUAGE FOR LAWYERS
Authors: Mikhalyova Svetlana N. 
Published: May 2023

In accordance with the Federal State Educational Standard of Higher Education, adopted by the order of the Ministry of Education and Science of the Russian Federation in February 2020, the implementation of various educational programs provides for the use of educational technologies. The article discusses the relevance of the search for modern technologies for teaching English to law students in the context of the implementation of the Federal State Educational Standard (3++). The author analyzes the possibilities of new pedagogical technologies and reveals their advantages. Based on the analysis of tasks for students, the author concluded that modern educational technologies contribute to the formation of foreign language competence in the process of teaching a professionally oriented foreign language course.
The article discusses modern educational technologies that are convenient to use when teaching a foreign language. Educational technologies include several convenient technological tools, an affordable, free, convenient service for work that can be used when preparing presentations, creating documents, various tables, as it offers a huge number of visual design options. The main results of the study are the scientific substantiation of the effectiveness of educational material.