bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early cessation of calcineurin inhibitors is feasible post–haploidentical blood stem cell transplant: the ANZHIT 1 study
Authors: Moore John , Hamad Nada , Gottlieb David , Bajel Ashish , Ritchie David , Yeung David , Greenwood Matthew , Purtill Duncan , Tran Steven , Solterbeck Annie , Aarons Donna , Kwan John 
Published: July 2023

Abstract
Haploidentical hematopoietic stem cell transplant (haplo-HSCT) using posttransplant cyclophosphamide (PTCy) is appropriate for those who lack matched donors. Most studies using PTCy have been retrospective making conclusions difficult. ANZHIT-1 was a phase 2 study conducted at 6 Australian allogeneic HSCT centers. The primary end points were disease-free and overall survival at 2 years after HSCT. The reduced-intensity conditioning (RIC) included fludarabine, cyclophosphamide, and 200 cGy total body irradiation, and the myeloablative conditioning (MAC) was IV fludarabine and busulfan. PTCy, MMF and a calcineurin inhibitor (CNI) were used for graft-versus-host disease (GVHD) prophylaxis. CNIs were weaned and ceased by day +120 in eligible patients on day 60. Patients (n = 78) with hematological malignancies were included in the study, with a median follow-up of 732 days (range, 28-1728). HSCT was RIC in 46 patients and MAC in 32 patients. Disease-free survival probability at 2 years was 67.5% (95% [CI], 53.2-85.6) for MAC recipients and 68.3% (95% CI, 56.3-83.01) for RIC recipients. Transplant-related mortality (TRM) on day 100 and year 1 was 4.9% (95% CI, 1.6-15.3) and 17.9% (95% CI, 8.8-36.5), respectively, in the MAC group compared with 3.1% (95% CI, 0.8.1-12) and 11.6% (95% CI, 6-22.4), respectively, in the RIC group. The median time for elective cessation of CNI was day 142.5 days, with no excess chronic GVHD (cGVHD) or mortality. Of the evaluable patients, 71.6% discontinued immunosuppression 12 months after transplant. This prospective haplo-HSCT trial using PTCY demonstrated encouraging survival rates, indicating that early CNI withdrawal is feasible and safe.
Ultrasensitive chimerism enhances measurable residual disease testing after allogeneic hematopoietic cell transplantation
Authors: Kanaan Sami B. , Urselli Francesca , Radich Jerald P. , Nelson J. Lee 
Published: July 2023

Abstract
Increasing mixed chimerism (reemerging recipient cells) after allogeneic hematopoietic cell transplant (allo-HCT) can indicate relapse, the leading factor determining mortality in blood malignancies. Most clinical chimerism tests have limited sensitivity and are primarily designed to monitor engraftment. We developed a panel of quantitative polymerase chain reaction assays using TaqMan chemistry capable of quantifying chimerism in the order of 1 in a million. At such analytic sensitivity, we hypothesized that it could inform on relapse risk. As a proof-of-concept, we applied our panel to a retrospective cohort of patients with acute leukemia who underwent allo-HCT with known outcomes. Recipient cells in bone marrow aspirates (BMAs) remained detectable in 97.8% of tested samples. Absolute recipient chimerism proportions and rates at which these proportions increased in BMAs in the first 540 days after allo-HCT were associated with relapse. Detectable measurable residual disease (MRD) via flow cytometry in BMAs after allo-HCT showed limited correlation with relapse. This correlation noticeably strengthened when combined with increased recipient chimerism in BMAs, demonstrating the ability of our ultrasensitive chimerism assay to augment MRD data. Our technology reveals an underappreciated usefulness of clinical chimerism. Used side by side with MRD assays, it promises to improve identification of patients with the highest risk of disease reoccurrence for a chance of early intervention.
Understanding a mass in the paraspinal region: an anatomical approach
Authors: Creze Maud , Ghaouche Jessica , Missenard Gilles , Lazure Thierry , Cluzel Guillaume , Devilder Matthieu , Briand Sylvain , Soubeyrand Marc , Meyrignac Olivier , Carlier Robert-Yves , Court Charles , Bouthors Charlie 
Published: July 2023

AbstractThe paraspinal region encompasses all tissues around the spine. The regional anatomy is complex and includes the paraspinal muscles, spinal nerves, sympathetic chains, Batson’s venous plexus and a rich arterial network. A wide variety of pathologies can occur in the paraspinal region, originating either from paraspinal soft tissues or the vertebral column. The most common paraspinal benign neoplasms include lipomas, fibroblastic tumours and benign peripheral nerve sheath tumours. Tumour-like masses such as haematomas, extramedullary haematopoiesis or abscesses should be considered in patients with suggestive medical histories. Malignant neoplasms are less frequent than benign processes and include liposarcomas and undifferentiated sarcomas. Secondary and primary spinal tumours may present as midline expansile soft tissue masses invading the adjacent paraspinal region. Knowledge of the anatomy of the paraspinal region is of major importance since it allows understanding of the complex locoregional tumour spread that can occur via many adipose corridors, haematogenous pathways and direct contact. Paraspinal tumours can extend into other anatomical regions, such as the retroperitoneum, pleura, posterior mediastinum, intercostal space or extradural neural axis compartment. Imaging plays a crucial role in formulating a hypothesis regarding the aetiology of the mass and tumour staging, which informs preoperative planning. Understanding the complex relationship between the different elements and the imaging features of common paraspinal masses is fundamental to achieving a correct diagnosis and adequate patient management. This review gives an overview of the anatomy of the paraspinal region and describes imaging features of the main tumours and tumour-like lesions that occur in the region.
PINNED: identifying characteristics of druggable human proteins using an interpretable neural network
Authors: Cunningham Michael , Pins Danielle , Dezső Zoltán , Torrent Maricel , Vasanthakumar Aparna , Pandey Abhishek 
Published: July 2023

AbstractThe identification of human proteins that are amenable to pharmacologic modulation without significant off-target effects remains an important unsolved challenge. Computational methods have been devised to identify features which distinguish between “druggable” and “undruggable” proteins, finding that protein sequence, tissue and cellular localization, biological role, and position in the protein–protein interaction network are all important discriminant factors. However, many prior efforts to automate the assessment of protein druggability suffer from low performance or poor interpretability. We developed a neural network-based machine learning model capable of generating druggability sub-scores based on each of four distinct categories, combining them to form an overall druggability score. The model achieves an excellent performance in separating drugged and undrugged proteins in the human proteome, with an area under the receiver operating characteristic (AUC) of 0.95. Our use of multiple sub-scores allows the assessment of potential protein targets of interest based on distinct contributors to druggability, leading to a more interpretable and holistic model to identify novel targets.
May microbial ecological baseline exist in continental groundwater?
Authors: Zhong Sining , Zhou Shungui , Liu Shufeng , Wang Jiawen , Dang Chenyuan , Chen Qian , Hu Jinyun , Yang Shanqing , Deng Chunfang , Li Wenpeng , Liu Juan , Borthwick Alistair G. L. , Ni Jinren 
Published: July 2023

Abstract
Background
Microbes constitute almost the entire biological community in subsurface groundwater and play an important role in ecological evolution and global biogeochemical cycles. Ecological baseline as a fundamental reference with less human interference has been investigated in surface ecosystems such as soils, rivers, and ocean, but the existence of groundwater microbial ecological baseline (GMEB) is still an open question so far.

Results
Based on high-throughput sequencing information derived from national monitoring of 733 newly constructed wells, we find that bacterial communities in pristine groundwater exhibit a significant lateral diversity gradient and gradually approach the topsoil microbial latitudinal diversity gradient with decreasing burial depth of phreatic water. Among 74 phyla dominated by Proteobacteria in groundwater, Patescibacteria act as keystone taxa that harmonize microbes in shallower aquifers and accelerate decline in bacterial diversity with increasing well-depth. Decreasing habitat niche breadth with increasing well-depth suggests a general change in the relationship among key microbes from closer cooperation in shallow to stronger competition in deep groundwater. Unlike surface-water microbes, microbial communities in pristine groundwater are predominantly shaped by deterministic processes, potentially associated with nutrient sequestration under dark and anoxic environments in aquifers.

Conclusions
By unveiling the biogeographic patterns and mechanisms controlling the community assembly of microbes in pristine groundwater throughout China, we firstly confirm the existence of GMEB in shallower aquifers and propose Groundwater Microbial Community Index (GMCI) to evaluate anthropogenic impact, which highlights the importance of GMEB in groundwater water security and health diagnosis.
Neodymium magnets migrated into an internal supravesical hernia: a rare case of foreign body ingestion in children
Authors: Ikegami Michiaki , Miyaki Yuichiro , Hamano Takashi , Taira Yurina , Takahashi Toshiaki 
Published: July 2023

Abstract
Background
Foreign body (FB) ingestion is a common event in children. The management of the ingested FB depends on the location, type, number, size of the FBs, patient age, and symptoms. Although most FBs pass spontaneously through the gastrointestinal tract without causing serious injuries, the ingestion of multiple high-powered magnetic pieces, especially neodymium magnets (NMs) increases the risks of morbidity and mortality. Supravesical hernia (SH) is rarely occurs in children, and few studies have reported SH in pediatric patients. We report an extremely rare case of ingested NMs that migrated into an internal SH in a pediatric patient.

Case presentation
A 3-year-old boy who had accidentally swallowed two NMs 3 days ago presented with vomiting and lower abdominal pain. X-ray imaging and computed tomography (CT) suspected the presence of a 1.0-cm radiopaque FB located in the terminal ileum dorsal side of the bladder. Although his abdominal pain was gradually getting better after oral feeding, repeat abdominal X-ray imaging showed that the FB was in a stagnant in position. Therefore, surgical intervention was planned to remove the FB 1 week after his admission. Under general anesthesia, laparoscopic and fluoroscopic examinations were performed and the cecum was found adhered to the retroperitoneum between the right medial umbilical fold and the right wall of the urinary bladder. The FB was presumed to be located at the tip of the incarcerated cecum in the retroperitoneal space. Peritoneum incision was started near the medial inguinal fossa, and the Retzius space was opened in a manner similar to the transabdominal pre-peritoneal approach for inguinal hernia repair. Consequently, the patient was diagnosed with internal SH with FB migration. The incarcerated cecum was pulled out, which revealed intestinal wall perforation. The FB remained in the retroperitoneal space in the pelvic cavity. The FB was easily removed using intestinal forceps and identified as combined two NMs. The postoperative course was good, and the patient was discharged on postoperative day 5.

Conclusions
We experienced an extremely rare case of a pediatric patient who swallowed multiple NMs that migrated into an internal SH, and the laparoscopic minimally invasive removal was successful.
Tropical climate change and its impact on horticultural plants in Enrekang District, South Sulawesi, Indonesia
Authors: YASSI AMIR , DEMMALLINO EYMAL B. , SULTANI HAIRUL RACHMAN 
Published: July 2023

Abstract.Yassi A, Demmallino EB, Sultani HR. 2023. Tropical climate change and its impact on horticultural plants in Enrekang District, South Sulawesi, Indonesia. Biodiversitas 24: 3073-3079. Global, regional, and local changes in climate are phenomenologically different and have distinct impacts on the horticultural and agricultural sectors. Therefore, this research aims to examine tropical climate change in South Sulawesi, Indonesia and analyze its impact on horticultural crops. Apost-positivistic interpretation framework of the case study tradition was used, while the location was determined by purposive sampling followed by comprehensive data collection. Data were analyzed using integrated techniques, and validity tests were carried outto assess thecredibility and reliability. The results indicated a local climate change marked by a differencein the pattern of distribution and intensity of extreme rainfall between 2015 and 2018. This was due toan increase in air temperature, which on average reached ±0.03731°C from the lowest of 22.50°C in 2011 to the highest of 23.12°C in 2020. In other words, it can be stated that the air temperature had increased by ±0.63°C in the last decade. The impact on various types of horticultural crops was marked by a decrease in the production of several kinds of Chinese cabbage,carrots, and green beans in 2015 as well asa very drastic reductionin potato crop production in 2018, while cabbage plants experienced a peak in production in 2018. Some types of horticultural crops such as shallots, cabbage and red chili began to stabilize and even increased their production in the aftermath of climate changeduring said period.
Solute carrier family 35 member A2 (SLC35A2) is a prognostic biomarker and correlated with immune infiltration in stomach adenocarcinoma
Authors: Huang Zigao , Yang Hong , Lao Jingmao , Deng Wei 
Published: July 2023

Background
Solute carrier family 35 member A2 (SLC35A2) located on the X chromosome is considered involved in the UDP-galactose transport from cytosol to Golgi apparatus and endoplasmic reticulum. It has been reported that the SLC35A2 expression is associated with carcinogenesis in recent studies, however, its specific roles in cancer progression have not been exhaustively elucidated. Herein, a system analysis was conducted to evaluate the role of SLC35A2 in prognostic, and immunology in stomach adenocarcinoma (STAD).

Methods
The TIMER, GEPIA, UALCAN, Kaplan–Meier Plotter were employed to explore the SLC35A2 expression pattern and prognostic value in STAD. Genomic alterations were searched through the MEXPRESS and cBioPortal platforms. The LinkedOmics, GEPIA and Metascape databases were employed to explore the biological processes. The TIMER and TISIDB websites were utilized to investigate the relationships between SLC35A2 expression and immune cell infiltration. The associations between SLC35A2 expression and tumor mutational burden (TMB), microsatellite instability (MSI) in pan-cancer were explored using the SangerBox database.

Results
Compared to the normal gastric mucosa, SLC35A2 expression was significantly increased in STAD tissues, accompanied by the robust relationships with tumor grade, histological subtypes, TP53 mutation status, TMB and prognosis. SLC35A2 and its co-expression genes played the primarily roles in purine metabolism and purinosome, including the asparagine N-linked glycosylation, protein processing in endoplasmic reticulum, regulation of transcription involved in G1/S transition of mitotic cell cycle, with the potential to participate in the regulation of VEGFA-VEGFR2 signaling pathway. Concurrently, SLC35A2 expression was correlated with macrophages and CD4+T lymphocytes infiltration in STAD.

Conclusions
Our study has proposed that SLC35A2 correlated with immune cell infiltration could serve as a prognostic biomarker in STAD.
An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of topiramate in human serum and plasma
Authors: Salzmann Linda , Spescha Tino , Singh Neeraj , Kobel Anja , Fischer Vanessa , Schierscher Tobias , Bauland Friederike , Geistanger Andrea , Risch Lorenz , Geletneky Christian , Seger Christoph , Taibon Judith 
Published: July 2023

Abstract

Objectives
Topiramate is an antiepileptic drug (AED) used for the monotherapy or adjunctive treatment of epilepsy and for the prophylaxis of migraine. It has several pharmacodynamic properties that contribute to both its clinically useful properties and observed adverse effects. Accurate measurement of its concentration is therefore essential for dose adjustment/optimisation of AED therapy. Our aim was to develop and validate a novel reference measurement procedure (RMP) for the quantification of topiramate in human serum and plasma.

Methods
An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) method in combination with a protein-precipitation-based sample preparation allows for quantification of topiramate in human serum and plasma. To assure traceability to SI units, quantitative nuclear magnetic resonance (qNMR) was applied to characterize the reference material used as primary calibrator for this RMP. Matrix effects were determined by performing a post-column infusion experiment and comparing standard line slopes. Accuracy and precision was evaluated performing an extensive five day precision experiment and measurement uncertainty was evaluated according Guide to the Expression of Uncertainty in Measurement (GUM).

Results
The method enabled topiramate quantification within the range of 1.20–36.0 μg/mL without interference from structurally related compounds and no evidence of a matrix effect. Intermediate precision was ≤3.2 % and repeatability was 1.4–2.5 % across all concentration levels. The relative mean bias was −0.3 to 3.5 %. Expanded measurement uncertainties for target value assignment (n=6) were found to be ≤2.9 % (k=2) independent of the concentration level and the nature of the sample.

Conclusions
In human serum and plasma, the RMP demonstrated high analytical performance for topiramate quantification and fulfilled the requirements on measurement uncertainty. Traceability to SI units was established by qNMR content determination of the topiramate, which was used for direct calibration of the RMP. This RMP is, therefore, fit for purpose for routine assay standardization and clinical sample evaluation.
S. S. Zimnitsky — the creator of the functional direction in internal medicine. The outset — research in gastroenterology
Authors: Оslopov V. N. , Khazova E. V. , Khasanov N. R. , Oslopova Yu. V. , Mishanina Yu. S. , Oslopova D. V. 
Published: July 2023

Semen Semenovich Zimnitsky is an outstanding Russian internist of the early XX century. He created a functional direction and made a significant contribution to internal medicine, infectiology, immunology and microbiology, but his studies of gastroenterology are fundamental. The Zimnitsky’s works on hepatology, pancreatic diseases, and balneology are interesting. S. S. Zimnitsky discovered 5 types of gastric secretion, which turned out to be reversible, and proposed a repeated breakfast. The pinnacle of his work is Zimnitsky’s test, which makes it possible to determine the renal function without resorting to any invasive procedures and in no way burdening the patient. The onset of test creation was laid by S. S. Zimnitsky in his doctoral dissertation devoted to gastroenterology.
The aims of humanities in the training of medical students on the example of history subjects
Authors: Chumakov V. I. 
Published: July 2023

The article is devoted to the study of the place of history subjects in the formation of general cultural competencies of medical students. A theoretical analysis of the topic in domestic and foreign history and pedagogical science has been carried out. The necessity of history subjects in the system of higher professional medical education is substantiated. Based on many years of experience, pedagogical technologies developed by the authors are presented that most fully form general cultural and professional competencies within historical subjects. The data of pedagogical research on the topic are given, the opinions of students on the content of history subjects, on the process of forming knowledge, skills and abilities of medical students are explicated. The practical significance of the study for improving the pedagogical process in a medical university is reflected.
Valores de referencia de estudios de electromiografía de los músculos masetero y temporal
Authors: Rozo Albarracín Andersson Lufandt 
Published: July 2023

Los músculos masetero y temporal tienen mucha relevancia en el proceso de masticación; además, suelen verse afectados con enfermedades mioarticulares, neurológicas, entre otras. El objetivo de este estudio es presentar los resultados de electromiografía con electrodo de aguja de los músculos masetero y temporal al momento de la masticación, evaluando los parámetros en amplitud y duración de los potenciales obtenidos. Se tomaron veintiséis individuos con previa valoración de odontología que descartaba alteraciones congénitas, defectos de la masticación y con dinamometría normal en momento de mayor oclusión, a los cuales se les realizó electromiografía de aguja en los músculos masetero y temporal en la máxima oclusión; los resultados fueron analizados bajo el estudio de los valores de amplitud máxima y mínima, así como duración ubicando en percentiles y cuadriles, buscando determinar valores que puedan considerarse normales en esta muestra. Al estudiar el músculo temporal se encontró que la duración normal se encuentra entre 4,75 y 6,487 mseg, mientras que la amplitud se encontraría entre 1572,05 uV y 1038,03 uV; en el caso del músculo masetero, se evidenció que la duración normal se encuentra entre 4,03 y 6,767 mseg, mientras que la amplitud se encontraría entre 2838,43 uV y 1864,635 uV. Este estudio da a conocer valores propios para nuestra población en lo que respecta a duración y amplitud de los potenciales de acción de unidad motora de los músculos temporal y masetero, los cuales concuerdan con los establecidos previamente como normales. En estudios previos realizados en otras partes del mundo, se encontró que la duración es menor que en las extremidades, pero la amplitud es similar, aunque con tendencia a valores inferiores al promedio.
The Innovative use of modified vacuum dressing in post vulvectomy wound
Authors: Adam MA , Lebese RP , Mbodi L 
Published: July 2023

Abstract:
We present a case of a patient who had a vulvectomy for large genital warts that could not be primarily closed but was successfully managed with a modified approach of the vacuum dressing until it was closed, allowed to heal, with a cosmetically acceptable outcome to the satisfaction of both the team and the patient.