bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract 561: Development of competing and non-competing fully human internalizing anti-Her2 monoclonal antibodies
Authors: Serrero Ginette , Yue Binbin , Dong Jianping , Dong Chun , Hayashi Jun 
Published: April 2023

Abstract
The human epidermal growth factor receptor (HER) family of receptors has been implicated in several human cancers. Among them, HER2 is overexpressed in several cancers with or without gene amplification with prognostic and predictive implications. In 15-30% of breast cancers, HER2 overexpression and amplification are associated with shorter disease-free and overall survivals along with resistance to hormonal agents as well as increased risk of brain metastasis. Two types of HER2 targeting have been developed: anti-HER2 monoclonal antibodies and small molecule tyrosine kinase inhibitors. Trastuzumab is a humanized anti-HER2 monoclonal antibody that binds to domain IV of HER2 extracellular segment and blocks its signaling. It is the first therapeutic antibody targeting Her2 overexpression approved by the FDA. More recently, two antibody drug conjugates using trastuzumab have been approved: Ado-Trastuzumab-Emtansine (Kadcyla) consisting of trastuzumab conjugated to the drug mertansine DM1 and fam-trastuzumab-deruxtecan-nhki (Enhertu to deliver the topoisomerase inhibitor deruxtecan. We are reporting here the development of fully human internalizing anti-Her2 antibodies that compete or not with trastuzumab for binding to Her2. These antibodies have been developed by immunizing fully human (TC-Mab mouse) mice with recombinant Her2 protein. After production of hybridoma secreting fully human immunoglobulins, the screening process included inhibition of binding of trastuzumab to Her2 by enzyme linked immunoassay and by Octet epitope binning as well as internalization assay. Several internalizing antibodies able to compete or not with trastuzumab and with high affinity (Kd ranging from 10−9 M to 10−12 M) were selected. They were next investigated for their ability to deliver a cytotoxic payload in HER2 overexpressing cells breast cancer cells such as SKBR3, BT474 and AU565. Two antibodies: one competing with trastuzumab and the other one non-competing were further characterized. Data related to these antibodies’ biochemical characteristics as well as their ability to inhibit proliferation in vitro and in vivo in mouse xenografts studies will be presented here. In conclusion, the use of fully human mice in our laboratory provides a powerful and attractive approach to develop fully human monoclonal antibodies against cancer targets allowing to by-pass the need for humanization and affinity maturation of antibodies.
Citation Format: Ginette Serrero, Binbin Yue, Jianping Dong, Chun Dong, Jun Hayashi. Development of competing and non-competing fully human internalizing anti-Her2 monoclonal antibodies [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 561.
A Political Time Rewritten: Revisiting the Founding Year of the Khitan Empire
Authors: Miao Runbo 
Published: April 2023

Abstract
Materials dating from the Liao dynasty usually record the founding year of the Khitan Empire as 907; this was done deliberately, rather than it being caused by the negligence of historians. The official history records of the Khitan Empire contain two completely different narratives of the founding year: the account of “Junji Taiyi deity Repeatedly Appearing” from the “Biography of Taizu” in Liaoshi reflects the original appearance of the history of the empire foundation period in the first year of the Shence era (916). Other records were reworded by later historians, which caused the contents of these originally clear records to become completely blurred and concealed. This rewriting process may have been completed in the thirteenth year of the Zhongxi era (1044) during Emperor Xingzong’s reign; the records advance Emperor Taizu’s rise to the throne to the year that the Tang dynasty was destroyed. That year was essential in reconstructing the founding history of the Khitan Empire and a narrative showed a strong tendency to orthodoxy in the late Liao dynasty. This article offers further reflection on the portrayal of a political epoch in historical records from a dynamic and productive angle.
Modulation of the Oceanic Mesoscale Activity by the Mesoscale Thermal Feedback to the Atmosphere
Authors: Renault Lionel , Masson S. , Oerder V. , Colas F. , McWilliams J. C. 
Published: April 2023

Abstract
Ocean mesoscale thermal feedback (TFB) is the influence of mesoscale sea surface temperature (SST) anomalies on the overlying atmosphere and its feedback to the ocean. Over the past few decades, TFB has been shown to affect the atmosphere by inducing low-level wind and surface stress anomalies and modulating ocean–atmosphere heat fluxes ubiquitously over the global oceans. These anomalies can alter the climate variability. However, it is not clear yet to what extent heat and momentum flux anomalies modulate the mesoscale ocean activity. Here, using coupled ocean–atmosphere mesoscale simulations over a realistic subtropical channel centered on the equator in which the TFB can be turned off by spatially smoothing the SST as seen by the atmosphere, we show that TFB can damp the mesoscale activity, with a more pronounced effect near the surface. This damping appears to be sensitive to the cutoff filter used: on average, the surface mesoscale activity is attenuated by 9% when smoothing the SST using an ∼1000-km cutoff but by only 2% when using an ∼350-km cutoff. We demonstrate that the mesoscale activity damping is primarily caused by a sink of available eddy potential energy that is controlled by the induced-anomalous heat fluxes, the surface stress anomalies having a negligible role. When TFB is neglected, the absence of sink of potential energy is partly compensated by a more negative eddy wind work. We illustrate that TFB filtering in a coupled model must be done carefully because it can also impact the large-scale meridional SST gradients and subsequently the mean large-scale wind stress curl and ocean dynamics.
Photothermal therapy of papillary thyroid cancer tumor xenografts with targeted thyroid stimulating hormone receptor antibody functionalized multiwalled carbon nanotubes
Authors: Lee Seung Soo , Oudjedi Fatma , Kirk Andrew G. , Paliouras Miltiadis , Trifiro Mark A. 
Published: April 2023

AbstractMultiwalled carbon nanotubes (MWCNTs) are being widely investigated in multiple biomedical applications including, and not limited to, drug delivery, gene therapy, imaging, biosensing, and tissue engineering. Their large surface area and aspect ratio in addition to their unique structural, optical properties, and thermal conductivity also make them potent candidates for novel hyperthermia therapy. Here we introduce thyroid hormone stimulating receptor (TSHR) antibody–conjugate–MWCNT formulation as an enhanced tumor targeting and light-absorbing device for the photoablation of xenografted BCPAP papillary thyroid cancer tumors. To ensure successful photothermal tumor ablation, we determined three key criteria that needed to be addressed: (1) predictive pre-operational modeling; (2) real-time monitoring of the tumor ablation process; and (3) post-operational follow-up to assess the efficacy and ensure complete response with minimal side effects. A COMSOL-based model of spatial temperature distributions of MWCNTs upon selected laser irradiation of the tumor was prepared to accurately predict the internal tumor temperature. This modeling ensured that 4.5W of total laser power delivered over 2 min, would cause an increase of tumor temperature above 45 ℃, and be needed to completely ablate the tumor while minimizing the damage to neighboring tissues. Experimentally, our temperature monitoring results were in line with our predictive modeling, with effective tumor photoablation leading to a significantly reduced post 5-week tumor recurrence using the TSHR-targeted MWCNTs. Ultimately, the results from this study support a utility for photosensitive biologically modified MWCNTs as a cancer therapeutic modality. Further studies will assist with the transition of photothermal therapy from preclinical studies to clinical evaluations.
De novo full-length transcriptome analysis of two ecotypes of Phragmites australis (swamp reed and dune reed) provides new insights into the transcriptomic complexity of dune reed and its long-term adaptation to desert environments
Authors: Cui Jipeng , Qiu Tianhang , Li Li , Cui Suxia 
Published: April 2023

Abstract
Background
The extremely harsh environment of the desert is changing dramatically every moment, and the rapid adaptive stress response in the short term requires enormous energy expenditure to mobilize widespread regulatory networks, which is all the more detrimental to the survival of the desert plants themselves. The dune reed, which has adapted to desert environments with complex and variable ecological factors, is an ideal type of plant for studying the molecular mechanisms by which Gramineae plants respond to combinatorial stress of the desert in their natural state. But so far, the data on the genetic resources of reeds is still scarce, therefore most of their research has focused on ecological and physiological studies.

Results
In this study, we obtained the first De novo non-redundant Full-Length Non-Chimeric (FLNC) transcriptome databases for swamp reeds (SR), dune reeds (DR) and the All of Phragmites australis (merged of iso-seq data from SR and DR), using PacBio Iso-Seq technology and combining tools such as Iso-Seq3 and Cogent. We then identified and described long non-coding RNAs (LncRNA), transcription factor (TF) and alternative splicing (AS) events in reeds based on a transcriptome database. Meanwhile, we have identified and developed for the first time a large number of candidates expressed sequence tag-SSR (EST-SSRs) markers in reeds based on UniTransModels. In addition, through differential gene expression analysis of wild-type and homogenous cultures, we found a large number of transcription factors that may be associated with desert stress tolerance in the dune reed, and revealed that members of the Lhc family have an important role in the long-term adaptation of dune reeds to desert environments.

Conclusions
Our results provide a positive and usable genetic resource for Phragmites australis with a widespread adaptability and resistance, and provide a genetic database for subsequent reeds genome annotation and functional genomic studies.
Patterns and predictors of public dental service utilisation among refugees in Victoria, Australia: a latent profile and multilevel analysis
Authors: Veginadu Prabhakar , Masood Mohd , Gussy Mark , Calache Hanny 
Published: April 2023

Abstract
Background
The purpose of the study was to explore, analyse, and describe the patterns of public dental service utilisation among the refugee populations in Victoria, Australia, and determine their predictors at the individual and contextual levels.

Methods
Data on the refugees who attended Victorian public dental services between July 2016 to June 2020 was gathered from the Dental Health Program dataset. Latent profile analysis was used to identify discrete groups among the refugee clientele with similar mean utilisation patterns across six indicator variables describing the attributes of dental services received and the site of care provision, over the study period. Multilevel multinomial logistic regression analysis was performed to examine the individual and contextual level correlates of the identified utilisation patterns.

Results
Six distinct profiles of public dental service utilisation were identified among the study population (n = 25,542). The largest group comprised refugees predominantly using restorative services under general course of care (38.10%), followed by extraction services under emergency course of care (23.50%). Only a small proportion were estimated as having a higher mean utilisation of preventive services under general course of care (9.10%). Multilevel analysis revealed that the following variables had a significant association with refugee utilisation pattern: at the individual-level – demographic and ethnic attributes including age, gender, region of birth, preferred language for communication, use of language interpreter services, and type of eligibility card; at the contextual-level – characteristics of refugees’ neighbourhood of residence including urbanicity, socioeconomic disadvantage, delivery of Refugee Health Program at the community health centres, and spatial accessibility to public dental services via driving and public transit modes of travel.

Conclusions
The study represents a significant step towards the development of an evidence-based knowledge around public dental service utilisation among Victorian refugees. Overall, the study findings reiterate the critical need for targeted strategies to promote the importance of routine dental visits, oral disease prevention, and timely intervention among refugee groups.
Factors associated with the closure of obstetric units in German hospitals and its effects on accessibility
Authors: Hoffmann Jan , Dresbach Till , Hagenbeck Carsten , Scholten Nadine 
Published: April 2023

Abstract
Background
An increase in regionalization of obstetric services is being observed worldwide. This study investigated factors associated with the closure of obstetric units in hospitals in Germany and aimed to examine the effect of obstetric unit closure on accessibility of obstetric care.

Methods
Secondary data of all German hospital sites with an obstetrics department were analyzed for 2014 and 2019. Backward stepwise regression was performed to identify factors associated with obstetrics department closure. Subsequently, the driving times to a hospital site with an obstetrics department were mapped, and different scenarios resulting from further regionalization were modelled.

Results
Of 747 hospital sites with an obstetrics department in 2014, 85 obstetrics departments closed down by 2019. The annual number of live births in a hospital site (OR = 0.995; 95% CI = 0.993–0.996), the minimal travel time between two hospital sites with an obstetrics department (OR = 0.95; 95% CI = 0.915–0.985), the availability of a pediatrics department (OR = 0.357; 95% CI = 0.126–0.863), and population density (low vs. medium OR = 0.24; 95% CI = 0.09–0.648, low vs. high OR = 0.251; 95% CI = 0.077–0.822) were observed to be factors significantly associated with the closure of obstetrics departments. Areas in which driving times to the next hospital site with an obstetrics department exceeded the 30 and 40 min threshold slightly increased from 2014 to 2019. Scenarios in which only hospital sites with a pediatrics department or hospital sites with an annual birth volume of ≥ 600 were considered resulted in large areas in which the driving times would exceed the 30 and 40 min threshold.

Conclusion
Close distances between hospital sites and the absence of a pediatrics department at the hospital site associate with the closure of obstetrics departments. Despite the closures, good accessibility is maintained for most areas in Germany. Although regionalization may ensure high-quality care and efficiency, further regionalization in obstetrics will have an impact on accessibility.
The value of partnership in patient-driven as well as in researcher-driven projects
Authors: Søndergaard Henning , Deele Malene , Agerskov Hanne , Lomborg Kirsten , Finderup Jeanette 
Published: April 2023

AbstractPatient involvement in health research is rarely driven solely by patients, who could be considered to have the highest degree of investment in such research. In the Kidney Connect project, the patients have been the driving force. This commentary considers the following questions: How did we, as patients, lead the work as the driving force in the project? What went well and what did not go so well from our perspective? How did the project compare with work driven by researchers? We argue that projects driven solely by either patients or researchers each have their own limitations. Projects driven solely by patients have some limitations in their robustness, rigour, and likelihood of publication. Nevertheless, a project driven solely by patients has been able to produce findings that are broadly comparable to a project driven solely by researchers that employed methods ensuring robustness and rigour. We suggest collaboration between patients and researchers also for projects driven by patients.
Automated whole-volume measurement of CT fat fraction of the pancreas: correlation with Dixon MR imaging
Authors: Tanabe Masahiro , Higashi Mayumi , Tanabe Masaya , Kawano Yosuke , Inoue Atsuo , Narikiyo Koji , Kobayashi Taiga , Ueda Takaaki , Ito Katsuyoshi 
Published: April 2023

Objectives: This study aimed to assess the feasibility of pancreatic steatosis quantification by automated whole-volume measurement of the fat fraction of the pancreas on CT in comparison to MRI using proton-density fat fraction (PDFF) techniques. Methods: Fifty-nine patients who underwent both CT and MRI were analyzed. Automated whole-volume measurement of pancreatic fat on unenhanced CT was performed by a histogram analysis with local thresholding. Three sets of CT fat volume fraction (FVF) (%) values with thresholds of −30 Hounsfield unit (HU), −20 HU and −10 HU were compared to MR-FVF (%) values measured on a PDFF map. Results: The median −30 HU CT-FVF, −20 HU CT-FVF, −10 HU CT-FVF and MR-FVF values of the pancreas were 8.6% (interquartile range (IQR), 11.3), 10.5% (IQR, 13.2), 13.4% (IQR, 16.1) and 10.9% (IQR, 9.7), respectively. The −30 HU CT-FVF (%), −20 HU CT-FVF (%) and −10 HU CT-FVF (%) of the pancreas showed a significant positive correlation with the MR-FVF (%) of the pancreas (ρ = 0.898, p < 0.001, ρ = 0.905, p < 0.001, ρ = 0.909, p < 0.001, respectively). The −20 HU CT-FVF (%) displayed reasonable agreement with the MR-FVF (%) with a low absolute fixed bias (mean difference, 0.32%; limit of agreement from −10.1 to 10.7%). Conclusion: The automated whole-volume measurement of the CT fat fraction of the pancreas using the threshold CT attenuation value of −20 HU may be a feasible, non-invasive, and convenient technique for quantifying pancreatic steatosis. Advances in knowledge: CT-FVF value of the pancreas had a positive correlation with the MR-FVF value. The −20 HU CT-FVF may be a convenient technique for quantifying pancreatic steatosis.
Migration background and COVID-19 related intensive care unit admission and mortality in the Netherlands: A cohort study
Authors: Ocak Gurbey , Khairoun Meriem , van Stigt Thans Martine , Meeder Danielle , Moeniralam Hazra , Dekker Friedo W. , Verhaar Marianne C. , Bos Willem Jan W. , Kaasjager Karin A. H. 
Published: April 2023

Background
Since the beginning of the SARS-CoV-2 pandemic, studies have been reporting inconsistently on migration background as a risk factor for COVID-19 outcomes. The aim of this study was to evaluate the association between migration background and clinical outcomes with COVID-19 in the Netherlands.

Methods
This cohort study included 2,229 adult COVID-19 patients admitted in two Dutch hospitals between February 27, 2020 and March 31, 2021. Odds ratios (ORs) for hospital admission, intensive care unit (ICU) admission and mortality with 95% confidence intervals (CIs) were calculated for non-Western (Moroccan, Turkish, Surinamese or other) persons as compared with Western persons in the general population of the province of Utrecht (the Netherlands) as source population. Furthermore, among hospitalized patients, Hazard ratios (HRs) with 95% CIs for in-hospital mortality and intensive care unit (ICU) admission were calculated using Cox proportional hazard analyses. Hazard ratios were adjusted for age, sex, body mass index, hypertension, Charlson Comorbidity Index, chronic corticosteroid use before admission, income, education and population density to investigate explanatory variables.

Results
Of the 2,229 subjects, 1,707 were of Western origin and 522 were of non-Western origin. There were 313 in-hospital deaths and 503 ICU admissions. As compared with persons with a Western origin in the general population of the province of Utrecht, the ORs for non-Western persons was 1.8 (95% CI 1.7–2.0) for hospitalization, 2.1 (95% CI 1.7–2.5) for ICU admission and 1.3 (95% CI 1.0–1.7) for mortality. Among hospitalized patients, HR for ICU admission was 1.1 (95% CI 0.9–1.4) and 0.9 (95% CI 0.7–1.3) for mortality for non-Western hospitalized persons as compared with hospitalized patients of Western origin after adjustment.

Conclusion
Non-Western persons, including Moroccan, Turkish and Surinamese subjects, had increased risks of hospital admission, ICU admission and COVID-19 related death on a population level. Among hospitalized COVID-19 patients, no association was found between migration background and ICU admission or mortality.
Hemispheric Quechua: language education and reclamation within diasporic communities in the United States
Authors: Mendoza-Mori Américo , Sprouse Rachel 
Published: April 2023

Abstract
Over the last two decades, the United States has increasingly become a site of Quechua language use and reclamation. Reclamation programs have emerged, both promoting the language and fostering community empowerment, particularly among Latinx youth. In this essay, we draw on our experiences as U.S.-based Quechua-language educators and organizers to explore the participation of diasporic Quechua reclamation movements in the global advance of the language. We frame these U.S.-based projects not as discrete entities, but as initiatives in constant connection with their counterparts in the Andes. This reflection piece provides a timeline of academic and community organizations in New York City, a global urban center with one of the largest bilingual Quechua-Spanish communities outside of the Andes. We conclude that these diasporic bottom-up language policy and planning (LPP) efforts are natural agents of dialogue on Quechua-language education and an integral part of the international Quechua reclamation movement.
МЕДІАТЕХНОЛОГІЇ ЯК ЕФЕКТИВНИЙ ЗАСІБ ПІДВИЩЕННЯ ЯКОСТІ ОСВІТНЬОГО ПРОЦЕСУ В ЗАКЛАДАХ ВИЩОЇ ОСВІТИ
Authors: ЧЕРВІНСЬКА ІННА , СТРУК АННА , ПРИТУЛЯК ОКСАНА , НИКОРАК ЯРОСЛАВ 
Published: April 2023

Автори розглядають питання розвитку медіаосвітніх технологій в умовах підготовки здобувачів освіти. Актуальність досліджуваної проблеми зумовлена великим освітньо-пізнавальним потенціалом сучасних медіатехнологій, які в педагогічній діяльності та освітній практиці виконують подвійну функцію: підвищення якості освітнього процесу під час вивчення різних дисциплін освітньої професійної програми шляхом високої ефективності якості навчального і розвивального потенціалу медіатехнологій – формування медійної грамотності студентів. Стаття присвячена проблематиці організації освітнього процесу у ЗВО за умов інтенсивного розвитку медіатехнологій, збільшення впливу медіаресурсів та впровадження ефективних медіапрактик у педагогічну діяльність. Обґрунтовано, що рівень медіаграмотності здобувача освіти виступає основним чинником формування успішного випускника-професіонала. Для вирішення окреслених завдань застосовано комплекс наукових методів, які забезпечують достовірність результатів і висновків. Наголошується, що медіатехнології можуть ефективно інтегруватися до освітнього контенту майже всіх навчальних дисциплін освітньої професійної програми певного рівня за умови дотримання дидактично-організаційних вимог щодо доцільності досягнення цілей і реалізації окреслених завдань медіаосвіти. Авторами вказується, що сучасне суспільство, де інформація набуває статусу соціокультурного й економічного ресурсів, вимагає підготовки компетентних спеціалістів, які вільно володіли б медіаосвітніми технологіями та ефективно їх застосовували в майбутній професійній діяльності. Дослідниками наголошується, що рівень медіаосвіти студентів повинен відповідати розвитку загального інтелектуального і професійного рівнів майбутнього фахівця певної галузі.