bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Tropical Cyclone Yaas on Coastal Regions of Odisha and West Bengal, India: An Assessment Using Sentinel Datasets
Authors: Das Bahadur , Dutta Dipanwita , Ray Ratnadeep 
Published: February 2025

ABSTRACTCoastal areas are increasingly susceptible to frequent cyclones driven by climate change. This leads to severe flooding, habitat loss, economic damage and community displacement, necessitating urgent adaptation measures. In this context, the present study aims to assess the impact of tropical cyclone Yaas on the coastal districts of Odisha and West Bengal combining SAR and optical satellite data. The Sentinel‐1 data was used for flood inundation analysis, allowing for the identification and mapping of areas affected by the cyclonic flooding. In addition, Sentinel‐2 data was employed for land use and land cover (LULC) analysis, enabling the evaluation of the cyclone's impact on various land cover classes. A fuzzy Analytic Hierarchy Process (AHP) was applied to analyse the changes in forest canopy cover. By integrating these diverse datasets and analyses, the study provides a holistic understanding of the cyclone's impact on the coastal region's environment and land cover. The findings reveal that the Yaas cyclonic flood affected an area of 2528.70 sq. km, accounting for 6.8% of the total region. In the coastal areas of West Bengal, more than 24% of cropland was affected particularly in the districts of Purba Medinipur, North 24 Parganas, Hoogly, Howrah and South 24 Parganas. In Odisha state, the most affected cropland areas were Bhadrak (945.11 sq. km) and Kendrapara (557.90 sq. km), while the districts of Bhadrak, Balasore, Jajpur, Khordha and Cuttack experienced the greatest impact on built‐up areas. The findings of this comprehensive study contribute to a deeper understanding of the magnitude and extent of tropical cyclone Yaas's impacts. This study can be useful for the development of effective mitigation and adaptation strategies that is, restoration of mangrove forests, introduction of salt‐tolerant crops and upgrading of existing embankments and levees to enhance the resilience of the coastal communities.
Adult brain cancer incidence patterns: A comparative study between Japan and Japanese Americans
Authors: Sigel Byron , Withrow Diana R. , Veiga Lene H. S. , Saito Eiko , Matsuda Tomohiro , Katanoda Kota 
Published: February 2025

AbstractAdult primary brain and central nervous system (CNS) cancers, though comprising only about 4% of new cancer diagnoses, significantly impact morbidity and mortality due to their low survival rates. Globally, brain and CNS tumor incidence varies considerably, with the United States exhibiting one of the highest rates and Japan among the lowest worldwide. In the United States, incidence rates differ by race, with higher rates in non‐Hispanic whites (NHW) and lower rates in Asian Americans and Pacific Islanders (AAPI). This study examines the incidence of malignant CNS tumors in Japan and Japanese Americans, comparing these groups to NHW and AAPI populations in the United States. We estimated age‐standardized incidence rates (ASR) of brain and CNS tumors among adults using data from the Monitoring of Cancer Incidence in Japan (MCIJ) and the U.S. Surveillance, Epidemiology, and End Results (SEER)‐9 registries from 2007 to 2014. Incidence rates were stratified by age, sex, and specific CNS tumor subtypes. Incidence rates of CNS tumors among Japanese (ASR: 3.66, 95% CI: 3.56–3.76) and Japanese Americans (ASR: 2.5, 95% CI: 2.13–3.05) were lower than among NHW (9.43, 95% CI, 9.31–9.56) and AAPI populations (ASR: 4.13, 95% CI: 3.94–4.33) in the United States. The same pattern was observed for CNS tumor subtypes and across age groups and sex. This study supports a genetic component in the risk of brain and CNS tumors, a cancer type with largely unknown etiology. By comparing incidence rates across populations, it contributes to understanding the balance of genetic and environmental risk factors in the development of these cancers.
Re‐evaluating prognostic indicators: The critical role of body composition and gene expression in endometrial cancer outcomes
Authors: Cuello Mauricio A. , Gómez‐Valenzuela Fernán , Ossandón Enrique , Wichmann Ignacio , Orlandini Elisa , Sáez Nicolás , Brañes Jorge , Kato Sumie , Ibañez Carolina 
Published: February 2025

AbstractObjectiveTo investigate the impact of body mass index (BMI), body composition (BC), and the expression of genes linked to obesity or lipid metabolism on the prognosis of endometrial cancer.MethodsWe conducted a comprehensive review of patients with confirmed endometrial cancer treated at the Pontificia Universidad Católica de Chile (PUC) and analyzed publicly available data from the endometrial cancer TCGA‐UCEC cohort. BC was assessed using computed tomography (CT) scans, and gene expression analysis was performed using RNA‐seq data. We evaluated the associations between BMI, BC, gene expression, and patient outcomes, including overall survival (OS) and progression‐free survival (PFS).ResultsOur study included 127 patients (67 from PUC and 60 from TCGA‐UCEC). BMI was not significantly associated with OS or PFS. However, BC metrics such as visceral adiposity and muscle mass were critical determinants of prognosis. We identified a 30‐gene risk score significantly associated with poorer PFS and OS, independent of other factors. Analysis of the tumor microenvironment (TME) revealed significant differences in immune cell composition and functional states between high‐ and low‐risk groups.ConclusionBMI alone is not a significant prognostic factor in endometrial cancer. Comprehensive assessments of BC, gene expression profiles, and the TME provide more accurate prognostic information and highlight potential therapeutic targets. These findings advocate for a shift towards personalized medicine, incorporating detailed phenotyping and molecular profiling to improve patient outcomes.
Time‐restricted feeding mitigates Alzheimer's disease‐associated cognitive impairments via a B. pseudolongum‐propionic acid‐FFAR3 axis
Authors: Zhao Yihang , Jia Mengzhen , Ding Chen , Bao Bingkun , Li Hangqi , Ma Jiabin , Dong Weixuan , Gao Rui , Chen Xuhui , Chen Jiao , Dai Xiaoshuang , Zou Yuanqiang , Hu Jun , Shi Lin , Liu Xuebo , Liu Zhigang 
Published: February 2025

AbstractTime‐restricted feeding (TRF) holds promise for alleviating cognitive decline in aging, albeit the precise mechanism via the gut‐brain axis remains elusive. In a clinical trial, we observed, for the first time, that a 4‐month TRF ameliorated cognitive impairments among Alzheimer's disease (AD) patients. Experiments in 5xFAD mice corroborated the gut microbiota‐dependent effect of TRF on mitigating cognitive dysfunction, amyloid‐beta deposition, and neuroinflammation. Multi‐omics integration linked Bifidobacterium pseudolongum (B. pseudolongum) and propionic acid (PA) with key genes in AD pathogenesis. Oral supplementation of B. pseudolongum or PA mimicked TRF's protective effects. Positron emission tomography imaging confirmed PA's blood‐brain barrier penetration, while knockdown of the free fatty acid receptor 3 (FFAR3) diminished TRF's cognitive benefits. Notably, we observed a positive correlation between fecal PA and improved cognitive function in an AD cohort, further indicating that TRF enhanced PA production. These findings highlight the microbiota‐metabolites‐brain axis as pivotal in TRF's cognitive benefits, proposing B. pseudolongum or PA as potential AD therapies.
A left‐posterior approach to the superior mesenteric artery during robot‐assisted pancreaticoduodenectomy
Authors: Kiritani Sho , Inoue Yosuke , Sato Takafumi , Sawa Yui , Kobayashi Kosuke , Oba Atsushi , Ono Yoshihiro , Ito Hiromichi , Takahashi Yu 
Published: February 2025

AbstractBackgroundA complete dissection around the superior mesenteric artery (SMA) with artery‐first concept is crucial during pancreaticoduodenectomy for periampullary cancers. The left‐posterior (LP) approach to the SMA may be effective during robot‐assisted pancreaticoduodenectomy (RPD), but data on its technical feasibility and clinical outcomes are limited.MethodsWe retrospectively reviewed 83 RPD patients utilizing the LP approach, dividing them into early (n = 42) and late (n = 41) groups to assess procedural maturity. The LP approach was initiated at the first phase of resection to achieve circumferential SMA dissection from the left and posterior aspect and early vascular control through ligation of the inferior pancreaticoduodenal artery (IPDA). Postoperative short‐term outcomes were compared between the two groups.ResultsThe late group demonstrated significantly shorter operative times (518 vs. 626 min; p < .01) and higher rates of IPDA ligation (90% vs. 71%; p = .03). The median blood loss in the late group was 50 mL (65 mL in the early group; p = .39). Lymph node retrieval number was 17 in both (p = .81), and R0 resection was achieved in all late group cases (96% in the early group; p = .35).ConclusionsWith experience from approximately 80 cases, the LP approach for RPD has enabled precise SMA dissection and early vascular control. A stable and super‐magnified caudal view provided by robotic surgery is particularly advantageous for this approach.
Current State of Evidence for Use of MRI in LI‐RADS
Authors: Kulkarni Ameya Madhav , Kruse Danielle , Harper Kelly , Lam Eric , Osman Hoda , Ansari Danyaal H. , Sivanesan Umaseh , Bashir Mustafa R. , Costa Andreu F. , McInnes Matthew , van der Pol Christian B. 
Published: February 2025

ABSTRACTThe American College of Radiology Liver Imaging Reporting and Data System (LI‐RADS) is the preeminent framework for classification and risk stratification of liver observations on imaging in patients at high risk for hepatocellular carcinoma. In this review, the pathogenesis of hepatocellular carcinoma and the use of MRI in LI‐RADS is discussed, including specifically the LI‐RADS diagnostic algorithm, its components, and its reproducibility with reference to the latest supporting evidence. The LI‐RADS treatment response algorithms are reviewed, including the more recent radiation treatment response algorithm. The application of artificial intelligence, points of controversy, LI‐RADS relative to other liver imaging systems, and possible future directions are explored. After reading this article, the reader will have an understanding of the foundation and application of LI‐RADS as well as possible future directions.
Isoliquiritigenin, an Extract from Licorice, Attenuates Dexamethasone‐Induced Muscle Atrophy via Akt/mTOR Pathway
Authors: Jiao Xin , Zhang Yuxin , Wang Zengguang , Chang Hanwen , Li Yongjin , Wang Binbin , Gan Yaokai , Gu Dongyun 
Published: February 2025

ABSTRACTMuscle atrophy is a pathological condition characterized by the excessive degradation of muscle proteins, leading to impaired physical performance. Isoliquiritigenin (ISL) is a promising extract known for its medical effects; however, its impact on muscle atrophy remains unclear. We investigated the effects of ISL on muscle atrophy both in vitro and in vivo. The results showed that 5‐µM ISL exhibited no significant cytotoxicity on C2C12 cells, as reflected by cell count kit‐8 and 5‐ethynyl‐2′‐deoxyuridine (EdU) tests. ISL increased the diameter of myotubes and downregulated forkhead box O proteins, muscle‐specific RING finger protein 1 (MuRF‐1), and Atrogin‐1 induced by Dexamethasone (Dex). ISL could also enhance the phosphorylation of Akt, the mammalian target of rapamycin (mTOR), eIF4E‐binding protein 1, and p70 S6 kinase in C2C12 myotubes. In animal experiments, ISL increased the muscle mass, improved the cross‐sectional area of muscles, and inhibited the expression of MuRF‐1 and Atrogin‐1 in muscle tissues. For physical performance, ISL enhanced grip strength and running endurance. ISL ameliorated Dex‐induced muscle atrophy both in vitro and in vivo, associated with increased diameter of myotubes and decreased Atrogin‐1 and MuRF‐1 expression via the Akt/mTOR signaling pathway. This suggested that ISL could be used as a natural drug for muscle atrophy.
Enhancing accuracy in proton therapy: The impact of geometric uncertainty models in head and neck cancer treatment
Authors: Zhang Ying , Chan Mark Ka Heng 
Published: February 2025

AbstractBackgroundAnatomical changes present a major source of uncertainty in head and neck (H&N) cancer treatment. Accurate modeling of these changes is important for enhancing treatment precision and supporting better outcomes.PurposeThe purpose of this study is to assess different anatomical uncertainty modeling methods in robust optimization for H&N cancer proton therapy.MethodsThis retrospective study involved five nasopharynx radiotherapy patients. We compared conventional robust optimization with anatomical robust optimization (aRO): (1) conventional robust optimization (cRO‐3 mm), which used 3 mm setup shift and 3% range uncertainty. (2) aRO_AM which used three predicted images from an AM capturing systematic anatomical changes, with a 1 mm setup shift and 3% range uncertainty. (3) aRO_PM, which used three predicted images from a probability model (PM) capturing the most probable deformations, also with a 1 mm setup shift and 3% range uncertainty. We assessed weekly dose coverage of the clinical target volumes (CTVs). Normal tissue complication probability (NTCP) for grade 2 xerostomia and grade 2 dysphagia were calculated using the accumulated nominal dose (without errors).ResultsaRO_PM outperformed cRO‐3 mm and aRO_AM, consistently achieving V94 95% for all cases across treatment weeks. Additionally, aRO_PM reduced the NTCP for grade 2 xerostomia by an average of 4.88 %, with a maximum reduction of 8.03%, and reduced the NTCP for grade 2 dysphagia by an average of 1.80%, with a maximum reduction of 4.23 %.ConclusionThe PM demonstrates potential for improving robust optimization by effectively managing anatomical uncertainties in H&N cancer proton therapy, thereby enhancing treatment effectiveness.
Effects of Myroxylon pereirae, Phenytoin, and Clinoptilolite After Pharyngocutaneous Fistula: An Experimental Animal Model
Authors: Pamuk Erim , Dogan Enes , Kurtulan Olcay , Güler Tezel Yeşim Gaye , Yiğit A. Arzu 
Published: February 2025

AbstractObjectiveThe objective of this study was to conduct a comparative evaluation of the effects of Myroxylon pereirae (MP), phenytoin, and clinoptilolite on wound healing in an experimental animal model for the treatment of pharyngocutaneous fistula (PCF).Study DesignProspective controlled animal study.SettingAnimal laboratory.MethodsForty‐four male Sprague–Dawley rats were randomly assigned to one of four groups: sham control group, MP group, phenytoin group, and clinoptilolite group. A PCF was created in each rat via surgical intervention, followed by a course of topical treatment administered twice daily for a period of 7 days. The healing of the fistula was evaluated both macroscopically and histopathologically.ResultsMacroscopic fistulae developed in 90% of the control group, 18% of the MP group, and 27% of the phenytoin group (P = .005). The phenytoin group had the lowest inflammation scores, which were significantly lower than the clinoptilolite and control groups (P = .006 and P = .001). The MP group had the highest levels of fibroblast proliferation and collagen accumulation (P < .001 and P = .001, respectively). The level of inflammation and amount of fibroblast proliferation, angiogenesis, and collagen accumulation in the clinoptilolite group was lower than in the control group, but none of these differences were significant statistically.ConclusionMP and phenytoin improved the healing of PCF, particularly by reducing the inflammation and promoting the of fibroblast proliferation and collagen accumulation. Clinoptilolite did not demonstrate a notable advantage in any of these parameters. These findings suggest that MP and phenytoin may serve as potential agents in the management of PCF.
A Little Known but Very Common Phenotype in Patients with Severe Congenital Neutropenia due to HAX1 Deficiency: Premature Ovarian Insufficiency
Authors: Kızılay Deniz Özalp , Karapınar Deniz Yılmaz , Karadaş Nihal , Karaoğlan Murat , Akbayram Sinan , Gökşen Damla , Gadashova Ayşe , Albayrak Serpil , Şahinoğlu Esra Pekpak , Orbak Zerrin , Bıçakçı Zafer , Akın Leyla , Albayrak Canan , Koç Cansu , Ünüvar Ayşegül , Anık Ahmet , Aral Yusuf Ziya , Cimbek Emine Ayça , Bahadır Ayşenur , Mete Cem , Özen Samim 
Published: February 2025

ABSTRACTBackgroundAutosomal recessive severe congenital neutropenia (SCN) has been associated with homozygous variants in the HAX1 gene. The aim of this cross‐sectional study was to evaluate the gonadal function and pubertal development in pediatric patients with SCN due to HAX1 gene variant (HAX1‐SCN).MethodsForty‐five patients, including 24 females (median age 11.3 [1.5–31] years, 13 pubertal, 11 prepubertal), and 21 males (median age 9.5 (3–18.8) years, 7 pubertal, 14 prepubertal), followed in seven centers, were included. POI is defined as a menstrual disturbance with increased follicle‐stimulating hormone (FSH) and low anti‐Mullerian hormone (AMH). We classified prepubertal female patients as impending POI when they had low AMH and high FSH values, indicating impaired ovarian function.ResultsA homozygous single nucleotide insertion (position 130–131insA) leading to a premature stop codon; p.Trp44*(c.132G>A) variant in HAX1 gene was detected in 42 (93.3%) affected individuals. Other homozygous variants were p.Arg86*(c.256C>T) and p.Glu60Aspfs*25(c.180delA). We detected elevated serum FSH levels in 10/11 (90.9%) of prepubertal female patients, supporting the diagnosis of impending POI, and in 12/13 (92.3%) of pubertal female patients, classifying them as POI. All female patients had low AMH levels. Male patients did not exhibit gonadal insufficiency.ConclusionsThis is the first and largest case series covering early childhood to evaluate patients with HAX1‐SCN for gonadal function. It has been observed that pubertal females develop POI, prepubertal females are at increased risk for gonadal failure, and male patients are not affected. Our results suggest that HAX1 has an important role in ovarian maturation and/or function.
Development of an antimicrobial material made of graphene oxide, polylactic acid/polyhydroxybutyrate, and CuO nanoparticles and its reprocessing effect on its properties
Authors: Quilaqueo Sergio , Bruna Julio E. , Galotto María J. , Guarda Abel , Rodríguez‐Mercado Francisco J. 
Published: February 2025

AbstractIn the present study nanocomposites made of polylactic acid (PLA), polyhydroxybutyrate (PHB), graphene oxide (GO), and copper (II) oxide (CuO) nanoparticles on three compositions (1, 3 and 5 wt%), obtained through melt extrusion were developed. Properties of the nanocomposites obtained in their first and second processing were evaluated, and characterized through colorimetry L*a*b*, Fourier‐transform infrared spectroscopy (FTIR), x‐ray diffraction (XRD), Differential scanning calorimetry (DSC), Thermogravimetric analysis (TGA), mechanical properties (ASTM D638 standard), scanning electron microscopy–energy‐dispersive x‐ray spectroscopy (SEM‐EDX) and antimicrobial activity under dynamic contact with ATCC25922 Escherichia coli. The obtained results of the first processing presented variations in color and crystallinity between different nanocomposites. Additionally, a higher antimicrobial activity was observed in the nanocomposites containing CuO, achieving up to 100% bacterial reduction at 3% nanoparticle content. When evaluating the influence of the second nanocomposites thermic processing, it could be seen an acceleration of the degradation process of the polymeric chains in addition to an improvement in the dispersion of the nanoparticles, which reduced their agglomeration and generated variations on the crystalline state of the nanocomposites.Highlights
The nanocomposites exhibit high antimicrobial activity.
GO and CuO nanoparticles show a tendency to agglomerate.
Reprocessing induces chain scission.
Reprocessing also enhances matrix homogenization.
The dispersion of the nanofiller reduced antimicrobial activity.
Exoproteomic Evidence for BcTSPO‐Mediated Regulation of Virulence Factors in Bacillus cereus
Authors: Duport Catherine , Armengaud Jean 
Published: February 2025

ABSTRACTBacillus cereus translocator protein (BcTSPO) is a transmembrane protein that plays a regulatory role in various Bacillus cereus phenotypes, potentially including its virulence. Given that the exoproteome is a major determinant of B. cereus virulence, particularly during the late stages of active growth, its analysis is crucial for understanding the regulatory functions of BcTSPO. In this study, we conducted a comparative analysis of the exoproteomes and cellular proteomes of a Δtspo mutant and the wild‐type ATCC 14579 strain during the late exponential and early stationary growth phases. Shotgun proteomics revealed that the absence of BcTSPO significantly depleted the exoproteome of secreted virulence factors, including the HBL, NHE, and CytK enterotoxins. Interestingly, the cholesterol‐dependent cereolysin O (CLO) was more abundant in the Δtspo exoproteome, indicating a distinct regulatory mechanism. The cellular proteome analysis further confirmed that BcTSPO positively regulates, likely indirectly, the synthesis of enterotoxins and negatively regulates the production of CLO. Additionally, the Δtspo exoproteome was enriched in moonlighting proteins, reflecting the impact of BcTSPO absence on cellular metabolism and stress responses. Overall, these findings highlight the critical role of BcTSPO in shaping the composition of both the exoproteome and cellular proteome, with significant implications for the virulence of B. cereus.
Properties and Cultivation of Fusarium spp. to Produce Mycoprotein as an Alternative Protein Source
Authors: Cheriaparambil Ramona , Grossmann Lutz 
Published: February 2025

ABSTRACTFusarium species offer a promising solution for cultivating mycoprotein, a sustainable alternative protein source with a variety of food applications. This review comprehensively examines the properties and cultivation methods of mycoprotein production using Fusarium biomass. Fusarium strains' capacity to produce biomass with high protein content, and the ability to utilize diverse substrates, make them ideal candidates for large‐scale cultivation and mycoprotein production. For maximum mycoprotein yields, the parameters that can be optimized for fermentation like temperature, pH, inoculum size, fermentation time, cultivation method, stirring speed, and substrate composition are explored in various studies enlisted here. The different methods of cultivation along with their advantages and disadvantages are also discussed. Furthermore, the nutritional composition and rheological attributes of Fusarium‐derived mycoprotein are reviewed, highlighting its potential as a viable substitute for animal‐based proteins in products like meat and dairy analogues. This review also sheds light on the Fusarium mycoprotein products already in the market and their sustainability values. Overall, mycoprotein derived from Fusarium species is a promising sustainable food ingredient, with opportunities for further improvement in developing more sustainable growth media and enhancing consumer acceptability.