bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting genotype-independent plant transformation by manipulating developmental regulatory genes and/or using nanoparticles
Authors: Yan Tingwei , Hou Quancan , Wei Xun , Qi Yuchen , Pu Aqing , Wu Suowei , An Xueli , Wan Xiangyuan 
Published: June 2023

AbstractKey messageThis review summarizes the molecular basis and emerging applications of developmental regulatory genes and nanoparticles in plant transformation and discusses strategies to overcome the obstacles of genotype dependency in plant transformation.AbstractPlant transformation is an important tool for plant research and biotechnology-based crop breeding. However, Plant transformation and regeneration are highly dependent on species and genotype. Plant regeneration is a process of generating a complete individual plant from a single somatic cell, which involves somatic embryogenesis, root and shoot organogeneses. Over the past 40 years, significant advances have been made in understanding molecular mechanisms of embryogenesis and organogenesis, revealing many developmental regulatory genes critical for plant regeneration. Recent studies showed that manipulating some developmental regulatory genes promotes the genotype-independent transformation of several plant species. Besides, nanoparticles penetrate plant cell wall without external forces and protect cargoes from degradation, making them promising materials for exogenous biomolecule delivery. In addition, manipulation of developmental regulatory genes or application of nanoparticles could also bypass the tissue culture process, paving the way for efficient plant transformation. Applications of developmental regulatory genes and nanoparticles are emerging in the genetic transformation of different plant species. In this article, we review the molecular basis and applications of developmental regulatory genes and nanoparticles in plant transformation and discuss how to further promote genotype-independent plant transformation.
The importance of wild plant resources in the Neolithic: a case study of the Late Neolithic lakeshore settlement of Grandson-Corcelettes, Les Pins (Switzerland)
Authors: Vandorpe Patricia , Akeret Örni , Kühn Marlu , Wick Lucia 
Published: June 2023

AbstractThe site of Grandson-Corcelettes, Les Pins is located at Lake Neuchâtel, Switzerland and was occupied during several phases of the Late Neolithic (between roughly 3000 and 2500 bc). Archaeobotanical analyses of the cultural layers revealed that, besides the cultivation of food plants, the inhabitants of Grandson used wild plant resources for many purposes. An estimation of the proportion of a selection of collected wild plants within the plant diet – based on calories – showed that they accounted for more than half of the calorific intake. This is in line with similar calculations performed for other Neolithic circum-Alpine settlements. The wild food plant spectrum indicates that most of them were gathered in the woodlands and semi-open habitats close to the village, whereas the riparian forest only seemed to play a marginal role. The analysis of macrofossils and pollen from dung pellets of goat and/or sheep showed that wild plants were also important as food for domestic animals, and that grazing took place in a variety of places, including forests, fallow fields and grassland-like habitats outside the village and throughout the year. Besides, there are indications that the nearby Jura mountains (reaching 1,600 m a.s.l. near the lakeshore villages) were exploited.
Depiction of an external classical field effects on a four-level W-configuration atom embedded in a coherent cavity field
Authors: Ali S. I. 
Published: June 2023

AbstractThis paper examines the dynamics of a W-configuration four-level atom in a quantized cavity field and the system driven by an external classical field. By applying some canonical transformations, we derive analytical solutions to the Schrödinger equation for the corresponding Hamiltonian. We have analyzed the impact of the external field and detuning parameters on the system’s relative entropy of coherence, Wigner function, and Pancharatnam phase. Our findings suggest that the external field parameter greatly affects the coherence of the system, whereas the detuning parameters may increase its maximum bounds. Furthermore, we have utilized the Wigner function as a tool to measure the quantumness and classicality of the system in its phase space. Our results indicate that the external field has a greater impact on the classicality of the system than the detuning parameters. Additionally, we have observed rapid oscillations in the dynamics of the Pancharatnam phase for large detuning values. It is worth noting that the external field reduces the number of phase jumps in the system.
Limb salvage in traumatic hemipelvectomy: case series with surgical management and review of the literature
Authors: Herold J. , Notov D. , Reeps C. , Schaser K. D. , Kamin K. , Mäder M. , Kleber C. 
Published: June 2023

Abstract
Background
Traumatic hemipelvectomies are rare and serious injuries. The surgical management was described in several case studies, with primary amputation often performed to save the patient's life.

Methods
We report of two survivors with complete traumatic hemipelvectomy resulting in ischemia and paralyzed lower extremity. Due to modern emergency medicine and reconstructive surgery, limb salvage could be attained. Long-term outcome with quality of life was assessed one year after the initial accident.

Results and conclusions
The patients were able to mobilize themselves and live an independent life. The extremities remained without function and sensation. Urinary continence and sexual function were present and the colostomy could be relocated in both patients. Both patients support limb salvage, even having difficulties and follow-up treatments. Concomitant cases are required to consolidate the findings.

Level of evidence
IV.
LAG3 in gastric cancer: it’s complicated
Authors: Ulase Dita , Behrens Hans-Michael , Krüger Sandra , Heckl Steffen M. , Ebert Ulrike , Becker Thomas , Röcken Christoph 
Published: June 2023

AbstractPurposeLymphocyte activation gene 3 (LAG3) is thought to contribute to T cell exhaustion within the tumor microenvironment of solid tumors. This study aimed to analyze the spatial distribution of LAG3 + cells in relation to clinicopathological and survival data in a large set of 580 primary resected and neoadjuvantly treated gastric cancers (GC).MethodsLAG3 expression was evaluated in tumor center and invasive margin using immunohistochemistry and whole-slide digital image analysis. Cases were divided into LAG3-low and LAG3-high expression groups based on (1) median LAG3 + cell density, (2) cut-off values adapted to cancer-specific survival usingCutoff Finderapplication.ResultsSignificant differences in spatial distribution of LAG3 + cells were observed in primarily resected GC, but not in neoadjuvantly treated GC. LAG3 + cell density showed evident prognostic value at following cut-offs: in primarily resected GC, 21.45 cells/mm2in tumor center (17.9 vs. 10.1 months,p = 0.008) and 208.50 cells/mm2in invasive margin (33.8 vs. 14.7 months,p = 0.006); and in neoadjuvantly treated GC, 12.62 cells/mm2(27.3 vs. 13.2 months,p = 0.003) and 123.00 cells/mm2(28.0 vs. 22.4 months,p = 0.136), respectively. Significant associations were found between LAG3 + cell distribution patterns and various clinicopathological factors in both cohorts. In neoadjuvantly treated GC, LAG3 + immune cell density was found to be an independent prognostic factor of survival (HR = 0.312, 95% CI 0.162–0.599,p < 0.001).ConclusionIn this study, a higher density of LAG3 + cells was associated with favorable prognosis. Current results support the need for extended analysis of LAG3. Differences in the distribution of LAG3 + cells should be considered, as they could influence clinical outcomes and treatment responses.Graphical abstract
Widespread distribution and altered pain processing in head and neck cancer survivors at long-term after treatment
Authors: Hernández Sofía Hernández , Guía Vanessa Gabriela Jerviz , Núñez Javier Martín , Ciuró Alejandro Heredia , Otero Alba Navas , Mohedo Esther Díaz , Valenza Marie Carmen 
Published: June 2023

Abstract
Purpose
Radiotherapy (RT) treatment in head and neck cancer (HNC) patients may induce long-term sequels as pain, which nowadays is not fully understand. Therefore, there is a need of characterization of pain features in HNC to enhance after oncology treatment management. Head and neck cancer survivors develop chronic pain after radiotherapy treatment. The purpose of the current study is to evaluate the presence of pain, pain distribution, and pain processing by means of patient reported outcomes and quantitative sensory testing.

Methods
Pain pressure threshold (PPT), temporal summation (TS), Brief Pain Inventory (BPI), Widespread Pain Index (WPI), The Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire, and EuroQol5D5L were assessed in 20 head and neck cancer survivors (sHNC) and 20 health-related sex and age-matched controls.

Results
The sHNC present lower PPT values in both the affected and non-affected side than did the healthy controls, especially in the widespread pain in the body, an altered TS in both affected and non-affected side and lower scores in quality of life and arm dysfunction.

Conclusions
Following radiotherapy treatment after 1 year, sHNC present widespread pain, hypersensitivity in the radiated area, altered pain processing, upper limb affection, and a QoL diminution. These data provide evidence that a peripheral and central sensitization is happening in sHNC. Future efforts should focus on preventing pain after oncologic treatment. The comprehension about pain and its features in sHNC enhance health professional understanding and allows to tailor an optimal patient-targeted pain treatment.
Histone modification as a drug resistance driver in brain tumors ⁎
Authors: Xi Guifa , Mania-Farnell Barbara , Lei Ting , Tomita Tadanori 
Published: June 2023

Abstract
Patients with brain tumors, specifically, malignant forms such as glioblastoma, medulloblastoma and ependymoma, exhibit dismal survival rates despite advances in treatment strategies. Chemotherapeutics, the primary adjuvant treatment for human brain tumors following surgery, commonly lack efficacy due to either intrinsic or acquired drug resistance. New treatments targeting epigenetic factors are being explored. Post-translational histone modification provides a critical regulatory platform for processes such as chromosome condensation and segregation, apoptosis, gene transcription, and DNA replication and repair. This work reviews how aberrant histone modifications and alterations in histone-modifying enzymes can drive the acquisition of drug resistance in brain tumors. Elucidating these mechanisms should lead to new treatments for overcoming drug resistance.
Penguin Huddling: A Continuum Model
Authors: Harris Samuel J. , McDonald N. R. 
Published: June 2023

AbstractPenguins huddling in a cold wind are represented by a two-dimensional, continuum model. The huddle boundary evolves due to heat loss to the huddle exterior and through the reorganisation of penguins as they seek to regulate their heat production within the huddle. These two heat transfer mechanisms, along with area, or penguin number, conservation, gives a free boundary problem whose dynamics depend on both the dynamics interior and exterior to the huddle. Assuming the huddle shape evolves slowly compared to the advective timescale of the exterior wind, the interior temperature is governed by a Poisson equation and the exterior temperature by the steady advection-diffusion equation. The exterior, advective wind velocity is the gradient of a harmonic, scalar field. The conformal invariance of the exterior governing equations is used to convert the system to a Polubarinova-Galin type equation, with forcing depending on both the interior and exterior temperature gradients at the huddle boundary. The interior Poisson equation is not conformally invariant, so the interior temperature gradient is found numerically using a combined adaptive Antoulas-Anderson and least squares algorithm. The results show that, irrespective of the starting shape, penguin huddles evolve into an egg-like steady shape. This shape is dependent on the wind strength, parameterised by the Péclet number Pe, and a parameter $\beta $
β
which effectively measures the strength of the interior self-generation of heat by the penguins. The numerical method developed is applicable to a further five free boundary problems.
Processing societal expectations: entrepreneurship initiative decision-making at a research university
Authors: Tuunainen Juha , Kantasalmi Kari 
Published: June 2023

AbstractDeploying systems-theoretical conceptuality, this paper improves understanding of the organisational consequences of the intensified societal engagement of a research university. Aligning its work with Luhmannian organisational analysis, it addresses the dynamic interplay between two modes of administrative decision-making communication, namely, the traditional professional administration and the New-Public-Management-oriented (NPM) managerial techniques. Our research observes how the politico-economic conditions of the society translate into the university’s decisions concerning an initiative to engage in start-up entrepreneurship. The article contributes to higher education literature by showing that the university’s professional administration is a discrete organisational function internally differentiated into specialised administrative branches, each of which operates according to a sense-making regime associated with its primary societal system reference, such as education, science and the economy. The article also demonstrates the structurally conditioned differences in branch-specific temporalisations of the entrepreneurial initiative during decision-making. Inspired by the Luhmannian view on temporality, we demonstrate how administrative decisions synchronise the varied structural time horizons within the university’s professional administration. Focus on temporality in decision-making thus allows us to see how the NPM-inspired managerial techniques are operationalised in administrative communication at universities. Consequently, the paper argues that university administration is a complex dynamic entity, which varyingly aligns itself to national policy scripts, and only selectively enacts features of a global trend known as NPM.
Combinatorics and preservation of conically stable polynomials
Authors: Codenotti Giulia , Gardoll Stephan , Theobald Thorsten 
Published: June 2023

AbstractGiven a closed, convex cone $$K\subseteq \mathbb {R}^n$$

K
⊆

R

n

, a multivariate polynomial $$f\in \mathbb {C}[\textbf{z}]$$

f
∈
C
[
z
]

is called K-stable if the imaginary parts of its roots are not contained in the relative interior of K. If K is the nonnegative orthant, K-stability specializes to the usual notion of stability of polynomials. We develop generalizations of preservation operations and of combinatorial criteria from usual stability toward conic stability. A particular focus is on the cone of positive semidefinite matrices (psd-stability). In particular, we prove the preservation of psd-stability under a natural generalization of the inversion operator. Moreover, we give conditions on the support of psd-stable polynomials and characterize the support of special families of psd-stable polynomials.
Sales prices, social rigidity and the second home property market
Authors: Hjalager Anne-Mette , Sørensen Michael Tophøj , Steffansen Rasmus Nedergård , Staunstrup Jan Kloster 
Published: June 2023

AbstractSecond homes are much valued as recreational resources and also as important commodities on the property market. This study examines the trading patterns and regional price development of Danish second homes from 1992 to 2020. Second home sales volumes and prices reflect the general economic booms and busts and also the possibilities to rent out the property on sharing platforms. However, across regional clusters and over time, property price developments suggest a significant social rigidity in preferences and prospects. The investment and financialization logics and the underlying guiding conspicuous consumption behavior has not changed as an effect of the increased demand during the early phases of the COVID-19 pandemic. When controlling for factors such as house and land plot size, building year, location attractiveness the strong social class and spatial rigidity is reproduced in the data. The shifting of wealth accumulated in the second homes between generations supports the same tendency, and taxation does not rebalance regional effects. Accordingly, only to a limited extent does owning a second home contribute to social equality, even if some second-home owners and policy makers tend to think otherwise. Economic measures in planning and governance portfolios are found to be negligible.
Parity Violation Energy Difference Calculation of Atropisomers
Authors: Vadivel Dhanalakshmi , Dondi Daniele 
Published: June 2023

AbstractEnantiomers have a different energy due to the parity violation effects. Up to now, these effects are difficult to calculate and their final effect on the choice of one enantiomer in the homochirality issue is still a matter of debate. Nevertheless, many scientists support the role of this tiny energy difference in the triggering of homochirality. In this work, we studied the energy difference in atropisomers, a class of stereoisomers in which the chirality is given by the block of rotation around one bond. Atropisomers might have a low energy barrier for the interconversion and this is interesting for the equilibration of the two enantiomers and the choice of the most stable enantiomer. Moreover, structures might be extended like in the case of polymers or crystals having helical framework and thus giving an additive effect on the parity violation energy of the whole structure. The parity violation energy difference here is discussed with the correlation on the general structure of the final molecule giving a qualitative model to predict the sign of local contributions of atoms.
Fineness of grain and the hylomorphism of experience
Authors: Settegast Sascha 
Published: June 2023

AbstractA central objection to McDowell’s conceptualism about empirical content concerns the fine-grained phenomenology of experience, which supposedly entails that the actual content of experience cannot be matched in its particularity by our concepts. While McDowell himself has answered this objection in recourse to the possibility of demonstrative concepts, his reply has engendered a plethora of further objections and is widely considered inadequate. I believe that McDowell’s critics underestimate the true force of his reply because they tend to read unrecognized empiricist presuppositions into his account of experience. To show this, I introduce a new hylomorphic reading of McDowell’s account of experience and argue that the objections to his reply all rest on a specific empiricist assumption, which is untenable because it conflates the form of experience with its content. Consequently, conceptualism so understood can resist all of these objections, as I attempt to show by systematizing and answering them.
Construction of prediction model for KRAS mutation status of colorectal cancer based on CT radiomics
Authors: Cao Yuntai , Zhang Jing , Huang Lele , Zhao Zhiyong , Zhang Guojin , Ren Jialiang , Li Hailong , Zhang Hongqian , Guo Bin , Wang Zhan , Xing Yue , Zhou Junlin 
Published: June 2023

Abstract
Background
In this study, we used computed tomography (CT)-based radiomics signatures to predict the mutation status of KRAS in patients with colorectal cancer (CRC) and to identify the phase of radiomics signature with the most robust and high performance from triphasic enhanced CT.

Methods
This study involved 447 patients who underwent KRAS mutation testing and preoperative triphasic enhanced CT. They were categorized into training (n = 313) and validation cohorts (n = 134) in a 7:3 ratio. Radiomics features were extracted using triphasic enhanced CT imaging. The Boruta algorithm was used to retain the features closely associated with KRAS mutations. The Random Forest (RF) algorithm was used to develop radiomics, clinical, and combined clinical–radiomics models for KRAS mutations. The receiver operating characteristic curve, calibration curve, and decision curve were used to evaluate the predictive performance and clinical usefulness of each model.

Results
Age, CEA level, and clinical T stage were independent predictors of KRAS mutation status. After rigorous feature screening, four arterial phase (AP), three venous phase (VP), and seven delayed phase (DP) radiomics features were retained as the final signatures for predicting KRAS mutations. The DP models showed superior predictive performance compared to AP or VP models. The clinical–radiomics fusion model showed excellent performance, with an AUC, sensitivity, and specificity of 0.772, 0.792, and 0.646 in the training cohort, and 0.755, 0.724, and 0.684 in the validation cohort, respectively. The decision curve showed that the clinical–radiomics fusion model had more clinical practicality than the single clinical or radiomics model in predicting KRAS mutation status.

Conclusion
The clinical–radiomics fusion model, which combines the clinical and DP radiomics model, has the best predictive performance for predicting the mutation status of KRAS in CRC, and the constructed model has been effectively verified by an internal validation cohort.
Impact-Dissipating Capacity of Fiber-Reinforced Polymer Samples, Fabricated by Fused Filament Fabrication
Authors: Tsamos Dimitris , Zyganitidis Ioannis , Fasnakis Dimitris , Ganatsios Stavros , Ntinas Nikolaos , Maropoulos Stergios , Tsouknidas Alexander 
Published: June 2023

AbstractThe use of additive manufacturing for the fabrication of sacrificial cladding is becoming increasingly popular as it facilitates the production of complex yet space-saving protective structures. Despite this, the effect of several structural parameters on their capacity to mitigate high-velocity impacts remains elusive. Toward this end, the shock-mitigating capacity of various short fiber-reinforced polymer samples was evaluated regarding impact velocity and mass (raging from 1 to 8.3 m/s and 5.5 to 7.5 Kg, respectively). Among the assessed parameters were peak force (measured to vary by up to 46.6%), max. and mean deceleration values (with max. differences documented at 29.5% and 48.2%, respectively) and cushion factor. As expected, the progressive crushing modes differed significantly across the spectrum of the tested samples. Structural failure involved the growth of inter- and intra-laminar cracks, fiber-matrix de-bonding and de-lamination, which was dependent on equivalent pore volume fraction and compressive strength. Increasing infill density led in most cases to higher peak forces during impact, as did the deposition of more solid peripheral layers, with the latter producing a superior deceleration plateau. Evaluated collectively, the results indicate that an infill density of 37% with 4 solid external (protective) layers exhibited the superior impact response among the tested samples.