bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spectral flow cytometry identifies distinct nonneoplastic plasma extracellular vesicle phenotype in glioblastoma patients
Authors: Aibaidula Abudumijiti (Zack) , Fain Cori E , Garcia Luz Cumba , Wier Annelise , Bouchal Samantha M , Bauman Megan M , Jung Mi-Yeon , Sarkaria Jann N , Johnson Aaron J , Parney Ian F 
Published: July 2023

Abstract

Background
Glioblastoma (GBM) is the most common malignant brain tumor and has a poor prognosis. Imaging findings at diagnosis and in response to treatment are nonspecific. Developing noninvasive assays to augment imaging would be helpful. Plasma extracellular vesicles (EVs) are a promising biomarker source for this. Here, we develop spectral flow cytometry techniques that demonstrate differences in bulk plasma EV phenotype between GBM patients and normal donors that could serve as the basis of a liquid biopsy.

Methods
Plasma EVs were stained for EV-associated tetraspanins (CD9/CD63/CD81), markers indicating cell of origin (CD11b/CD31/CD41a/CD45), and actin/phalloidin (to exclude cell debris). EVs were analyzed using spectral flow cytometry. Multiparametric analysis using t-distributed stochastic neighbor embedding (t-SNE) and self-organizing maps on flow cytometry data (FlowSOM) was performed comparing GBM and normal donor (ND) plasma EVs.

Results
Size exclusion chromatography plus spectral-based flow cytometer threshold settings enriched plasma EVs while minimizing background noise. GBM patients had increased CD9+, CD63+, CD81+, and myeloid-derived (CD11b+) EVs. Multiparametric analysis demonstrated distinct surface marker expression profiles in GBM plasma EVs compared to ND EVs. Fifteen plasma EV sub-populations differing in size and surface marker expression were identified, six enriched in GBM patients and two in normal donors.

Conclusions
Multiparametric analysis demonstrates that GBM patients have a distinct nonneoplastic plasma EV phenotype compared to ND. This simple rapid analysis can be performed without purifying tumor EVs and may serve as the basis of a liquid biopsy.
Estimating the Cost of Delivering Tobacco Cessation Intervention Package at Noncommunicable Disease Clinics in Two Districts of North India
Authors: Bhatt Garima , Goel Sonu , Kiran Tanvi , Grover Sandeep , Medhi Bikash , Singh Gurmandeep , Singh Gill Sandeep 
Published: July 2023

AbstractIntroductionIntegrated care is likely to improve outcomes in strained healthcare systems while limiting costs. NCD clinics were introduced under the “National Programme for Prevention and Control of Cancer, Diabetes, Cardiovascular Disease, and Stroke” (NPCDCS) in India; however, there is limited literature on the costs of delivering tobacco cessation interventions within NPCDCS. One of the study’s objectives was to estimate the cost of delivering a culturally specific patient-centric behavioral intervention package in two district-level NCD clinics in Punjab, India.MethodsCosting was undertaken using the health systems perspective. A top-down or financial costing approach and a bottom-up or activity-based approach were employed at each step of development and implementation. The opportunity cost was used to include the cost of human resources, infrastructure, and capital resources used. All infrastructure and capital costs were annualized using a 3% annual discount rate. Four additional scenarios were built up concerning three major components to reduce costs further when rolled out on a large scale.ResultsThe cost of intervention package development, human resource training, and unit cost of implementation were estimated to be INR 6,47,827 (USD 8,874); INR 134,002 (USD 1810); and INR 272 (USD 3.67), respectively. Based on our sensitivity analysis results, the service delivery cost varied from INR 184 (USD 2.48) to INR 326 (USD 4.40) per patient.ConclusionThe development costs of the intervention package accounted for the majority proportion of the total cost. Of the total unit cost of implementation, the telephonic follow-up, human resources, and capital resources were the major contributory components.ImplicationsThe current study aims to fill gaps by estimating the unit-level health systems cost of a culturally sensitive, disease-specific, and patient-centric tobacco cessation intervention package delivered at the outpatient settings of NCD clinics at the secondary level hospital, which represents a major link in the health care system of India. Findings from this study could be used to provide supportive evidence to policymakers and program managers for rolling out such interventions in established NCD clinics through the NPCDCS program of the Indian Government.
Acceptability, Feasibility, and Appropriateness of Implementation of Long-acting Injectable Antiretrovirals: A National Survey of Ryan White Clinics in the United States
Authors: Tarfa Adati , Sayles Harlan , Bares Sara H , Havens Joshua P , Fadul Nada 
Published: July 2023

Abstract

Background
The approval of long-acting injectable cabotegravir/rilpivirine (LAI CAB/RPV) heightened the urgency of ensuring effective implementation. Our study assesses readiness and barriers to implement LAI CAB/RPV across Ryan White–funded clinics in the United States.

Methods
We conducted a cross-sectional survey between December 2020 and January 2021 using validated 4-item measures: acceptability of intervention measure (AIM), intervention appropriateness measure (IAM), and feasibility of intervention measure (FIM). Associations between measures and clinic characteristics were evaluated via Spearman rank correlations. A 5-point Likert scale ranked potential barriers of implementation responses. Open-ended questions were analyzed through a thematic approach.

Results
Of 270 clinics, 44 (16%) completed the survey: 38% federally qualified health centers, 36% academic, 20% community-based organizations, 14% hospital outpatient, and 9% nonprofit. Means (SD; range) were as follows: AIM, 17.6 (2.4; 12–20); IAM, 17.6 (2.4; 13–20); and FIM, 16.8 (2.9; 7–20). Twenty percent were not at all ready to implement LAI CAB/RPV, and 52% were slightly or somewhat ready. There was a significant association between AIM and the proportion of Medicaid patients (AIM, rho = 0.312, P = .050). Community-based organizations scored the highest readiness measures (mean [SD]: AIM, 19.50 [1.41]; IAM, 19.25 [1.49]; FIM, 19.13 [1.36]) as compared with other clinics. Implementation barriers were cost and patients’ nonadherence to visits.

Conclusions
There is variability of readiness yet high levels of perceived acceptability and appropriateness of implementing LAI CAB/RPV among Ryan White clinics, necessitating tailored interventions for successful implementation. A special focus on addressing the barriers of adherence and the cost of implementation is needed.
Responding to parental concern about children’s reading
Authors: Bryant Katelyn J , Joanisse Marc F , Archibald Lisa M D 
Published: July 2023

Abstract

Objectives
This study explored if parents are accurate in their reading concerns, with implications for paediatric practice.

Methods
Parents of school-aged children in 34 schools in Ontario, Canada responded to a questionnaire about their children’s academic development (27% response), and their children participated in standardized, norm-referenced tests of word reading and an oral sentence recall task. Parental concern status was retrospectively cross-referenced with reading difficulty status (&lt;25th percentile on the word reading tests) for grade 2 children with complete data (n = 294); diagnostic accuracy was evaluated. Demographic and reading-related characteristics of children whose parents’ concern did not match their reading difficulty status were explored.

Results
Approximately 22% of parents endorsed concern, while 18.5% of children had word reading difficulty. Parental concern status accurately corresponded with reading difficulty status in 81% of cases (95% CI: 76% to 85%). Specificity was 85% (95% CI: 80% to 90%), while sensitivity was 59% (95% CI: 44% to 73%). Children of unconcerned parents rarely had word reading problems (NPV 91%; 95% CI: 88% to 94%), while children with concerned parents had word reading difficulty in 45% of cases (PPV; 95% CI: 36% to 54%). Apparent inaccuracy (i.e., mismatch between parental concern and reading difficulty status) was related to children’s word reading, oral sentence recall skills, and English language learner (ELL) status, but not child age, sex, or parent education.

Conclusions
Many parents of grade 2 children endorse reading concerns. Parental concern is an overall accurate screener for word reading difficulty, although some children will be missed. Evidence-informed recommendations for responding to parental concern are provided.
The CALCINEURIN B-LIKE 4/CBL-INTERACTING PROTEIN 3 module degrades repressor JAZ5 during rose petal senescence
Authors: Chen Changxi , Ma Yanxing , Zuo Lanxin , Xiao Yue , Jiang Yunhe , Gao Junping 
Published: July 2023

Abstract
Flower senescence is genetically regulated and developmentally controlled. The phytohormone ethylene induces flower senescence in rose (Rosa hybrida), but the underlying signaling network is not well understood. Given that calcium regulates senescence in animals and plants, we explored the role of calcium in petal senescence. Here, we report that the expression of calcineurin B-like protein 4 (RhCBL4), which encodes a calcium receptor, is induced by senescence and ethylene signaling in rose petals. RhCBL4 interacts with CBL-interacting protein kinase 3 (RhCIPK3), and both positively regulate petal senescence. Furthermore, we determined that RhCIPK3 interacts with the jasmonic acid response repressor jasmonate ZIM-domain 5 (RhJAZ5). RhCIPK3 phosphorylates RhJAZ5 and promotes its degradation in the presence of ethylene. Our results reveal that the RhCBL4-RhCIPK3-RhJAZ5 module mediates ethylene-regulated petal senescence. These findings provide insights into flower senescence, which may facilitate innovations in postharvest technology for extending rose flower longevity.
Evolution of the spinach sex-linked region within a rarely recombining pericentromeric region
Authors: She Hongbing , Liu Zhiyuan , Li Shufen , Xu Zhaosheng , Zhang Helong , Cheng Feng , Wu Jian , Wang Xiaowu , Deng Chuanliang , Charlesworth Deborah , Gao Wujun , Qian Wei 
Published: July 2023

Abstract
Sex chromosomes have evolved independently in many different plant lineages. Here, we describe reference genomes for spinach (Spinacia oleracea) X and Y haplotypes by sequencing homozygous XX females and YY males. The long arm of 185-Mb chromosome 4 carries a 13-Mb X-linked region (XLR) and 24.1-Mb Y-linked region (YLR), of which 10 Mb is Y specific. We describe evidence that this reflects insertions of autosomal sequences creating a “Y duplication region” or “YDR” whose presence probably directly reduces genetic recombination in the immediately flanking regions, although both the X and Y sex-linked regions are within a large pericentromeric region of chromosome 4 that recombines rarely in meiosis of both sexes. Sequence divergence estimates using synonymous sites indicate that YDR genes started diverging from their likely autosomal progenitors about 3 MYA, around the time when the flanking YLR stopped recombining with the XLR. These flanking regions have a higher density of repetitive sequences in the YY than the XX assembly and include slightly more pseudogenes compared with the XLR, and the YLR has lost about 11% of the ancestral genes, suggesting some degeneration. Insertion of a male-determining factor would have caused Y linkage across the entire pericentromeric region, creating physically small, highly recombining, terminal pseudoautosomal regions. These findings provide a broader understanding of the origin of sex chromosomes in spinach.
LncRNA ZNNT1 induces p53 degradation by interfering with the interaction between p53 and the SART3-USP15 complex
Authors: Taniue Kenzui , Oda Takeaki , Hayashi Tomoatsu , Kamoshida Yuki , Takeda Yasuko , Sugawara Anzu , Shimoura Yuki , Negishi Lumi , Nagashima Takeshi , Okada-Hatakeyama Mariko , Kawamura Yoshifumi , Goshima Naoki , Akimitsu Nobuyoshi , Akiyama Tetsu 
Published: July 2023

Abstract
Mammalian genomes encode large number of long noncoding RNAs (lncRNAs) that play key roles in various biological processes, including proliferation, differentiation, and stem cell pluripotency. Recent studies have addressed that some lncRNAs are dysregulated in human cancers and may play crucial roles in tumor development and progression. Here, we show that the lncRNA ZNNT1 is required for the proliferation and tumorigenicity of colon cancer cells with wild-type p53. ZNNT1 knockdown leads to decreased ubiquitination and stabilization of p53 protein. Moreover, we demonstrate that ZNNT1 needs to interact with SART3 to destabilize p53 and to promote the proliferation and tumorigenicity of colon cancer cells. We further show that SART3 is associated with the ubiquitin-specific peptidase USP15 and that ZNNT1 may induce p53 destabilization by inhibiting this interaction. These results suggest that ZNNT1 interferes with the SART3-USP15 complex-mediated stabilization of p53 protein and thereby plays important roles in the proliferation and tumorigenicity of colon cancer cells. Our findings suggest that ZNNT1 may be a promising molecular target for the therapy of colon cancer.
Electron wave functions in beta-decay formulas revisited (II): Completion including recoil-order and induced currents
Authors: Horiuchi Wataru , Sato Toru , Uesaka Yuichi , Yoshida Kenichi 
Published: July 2023

Abstract
We present complete formulas of the allowed and first-forbidden transitions of nuclear beta decay taking into account the recoil-order and induced currents up to the next-to-leading order (NLO). The longitudinal part of the vector current is cleared away by the use of the conservation of the vector current for the multipole operators of the natural-parity transitions, which makes the effect of the meson exchange current for the vector current as small as possible. The formula is transparent enough to be applied to various beta-decay processes. As a numerical demonstration, we apply our formulas to the beta decay of a neutron-rich nucleus 160Sn. We find that the NLO corrections amount to 10–20 percent of the total decay rate, whereas the induced currents alter the rate at most by 5 percent.
Derivation of transition density from the observed 4He(e, e′)4He(02+) form factor raising the α-particle monopole puzzle
Authors: Kamimura Masayasu 
Published: July 2023

Abstract
Recently, the monopole transition form factor of the electron-scattering excitation of the $0^+_2$ state (Ex = 20.21 MeV) of the 4He nucleus was observed over a broad momentum transfer range ($0.5 \le q^2 \le 5.0 \, {\rm fm}^{-2}$) with dramatically improved precision compared with older sets of data; modern nuclear forces, including those derived from the chiral effective field theory, failed to reproduce the form factor, which is called the α-particle monopole puzzle. To resolve this puzzle by improving the study of the spatial structure of the $0^+_2$ state, we derive in this letter a possible $0^+_1\!\rightarrow \! 0^+_2$ transition density ρtr(r) for r ≳ 1 fm from the observed form factor. The shape of the transition density is significantly different from that obtained theoretically in the literature.
New insights into kidney disease after COVID-19 infection and vaccination: histopathological and clinical findings
Authors: Li Yebei , Gong Yan , Xu Gaosi 
Published: July 2023

Summary
In addition to its pulmonary effects, coronavirus disease 2019 (COVID-19) has also been found to cause acute kidney injury (AKI), which has been linked to high mortality rates. In this review, we collected data from 20 clinical studies on post-COVID-19-related AKI and 97 cases of AKI associated with COVID-19 vaccination. Acute tubular injury was by far the most common finding in the kidneys of patients with COVID-19-related AKI. Among patients hospitalized for COVID-19, 34.0% developed AKI, of which 59.0%, 19.1% and 21.9% were Stages 1, 2 and 3, respectively. Though kidney disease and other adverse effects after COVID-19 vaccination overall appear rare, case reports have accumulated suggesting that COVID-19 vaccination may be associated with a risk of subsequent kidney disease. Among the patients with post-vaccination AKI, the most common pathologic findings include crescentic glomerulonephritis (29.9%), acute tubular injury (23.7%), IgA nephropathy (18.6%), antineutrophil cytoplasmic autoantibody-associated vasculitis (17.5%), minimal change disease (17.5%) and thrombotic microangiopathy (10.3%). It is important to note that crescentic glomerulonephritis appears to be more prevalent in patients who have newly diagnosed renal involvement. The proportions of patients with AKI Stages 1, 2 and 3 after COVID-19 vaccination in case reports were 30.9%, 22.7% and 46.4%, respectively. In general, clinical cases of new-onset and recurrent nephropathy with AKI after COVID-19 vaccination have a positive prognosis. In this article, we also explore the underlying pathophysiological mechanisms of AKI associated with COVID-19 infection and its vaccination by describing key renal morphological and clinical features and prognostic findings.
Diagnostic Reasoning Competency and Accuracy by Nurse Practitioner Students Following the Use of Structured Reflection in Simulation
Authors: Griffith Patricia B. , Mariani Bette , Kelly Michelle M. 
Published: July 2023

Abstract

AIM
The aim of this study was to examine the effect of structured reflection used during a simulated patient’s diagnostic workup on diagnostic reasoning competency and accuracy and explore participants’ cognitive bias experience and perceived utility of structured reflection.

BACKGROUND
Reasoning flaws may lead to diagnostic errors. Medical learners who used structured reflection demonstrated improved diagnosis accuracy.

METHOD
Embedded mixed-methods experiment examined diagnostic reasoning competency and accuracy of nurse practitioner students who did and did not use structured reflection. Cognitive bias experience and perceptions of structured reflection’s utility were explored.

RESULTS
Diagnostic Reasoning Assessment mean competency scores and categories were not changed. Accuracy trended toward improvement with structured reflection. The theme, diagnostic verification, prompted diagnosis change by both structured reflection users and control participants.

CONCLUSION
Despite no changes in quantitative outcomes, explicit users of structured reflection believed that this strategy is helpful to their reasoning, and control participants used the strategy’s components with the same noted benefits.
Implicit Bias in Nursing: Student Reflections
Authors: Hanks Jacquie , Van De Graaff Whitney , Hamzhie Chelsea , Koziol Dana , Williams Jacque 
Published: July 2023

Abstract
Implicit bias can lead to health disparities related to a patient’s race, religion, sexual identity, and mental illness. Students responded to the Implicit Association Test for race followed by a structured reflection. Student reflections were evaluated qualitatively. Results hold a key to future educational interventions/training to help nursing students become consciously aware of implicit biases and choose behaviors that are non-biased.
Myocardial Protection by Desflurane: From Basic Mechanisms to Clinical Applications
Authors: Qin Han , Zhou Jing 
Published: July 2023

Abstract:
Coronary heart disease is an affliction that is common and has an adverse effect on patients' quality of life and survival while also raising the risk of intraoperative anesthesia. Mitochondria are the organelles most closely associated with the pathogenesis, development, and prognosis of coronary heart disease. Ion abnormalities, an acidic environment, the production of reactive oxygen species, and other changes during abnormal myocardial metabolism cause the opening of mitochondrial permeability transition pores, which disrupts electron transport, impairs mitochondrial function, and even causes cell death. Differences in reliability and cost-effectiveness between desflurane and other volatile anesthetics are minor, but desflurane has shown better myocardial protective benefits in the surgical management of patients with coronary artery disease. The results of myocardial protection by desflurane are briefly summarized in this review, and biological functions of the mitochondrial permeability transition pore, mitochondrial electron transport chain, reactive oxygen species, adenosine triphosphate-dependent potassium channels, G protein–coupled receptors, and protein kinase C are discussed in relation to the protective mechanism of desflurane. This article also discusses the effects of desflurane on patient hemodynamics, myocardial function, and postoperative parameters during coronary artery bypass grafting. Although there are limited and insufficient clinical investigations, they do highlight the possible advantages of desflurane and offer additional suggestions for patients.
Imported Malaria in Children: A Study Over an 11-Year Period in Brussels
Authors: Selimaj Kontoni Valbona , Goetghebuer Tessa , Hainaut Marc , Vanderfaeillie Anna , Nguyen Vo Thanh Phuong , Jourdain Sarah , Pace David 
Published: July 2023

Background:
Malaria is a major global public health concern in endemic countries and imported childhood malaria is increasing in malaria non-endemic countries.

Methods:
This was a retrospective case review of all laboratory-confirmed malaria cases in children 0–16 years admitted between 2009 and 2019 in 2 large university teaching Hospitals in Brussels.

Results:
A total of 160 children with a median age of 6.8 years (range 5–191 months) were included. We identified 109 (68%) children living in Belgium who had acquired malaria during their visit to malaria-endemic countries to visiting friends and relatives (VFRs), 49 children (31%) visitors or newly installed migrants, and 2 Belgian tourists. Peak seasonal incidence occurred between August and September. Plasmodium falciparum was responsible for 89% of all malaria cases. Almost 80% of children living in Belgium visited a travel clinic for advice, but only one-third reported having taken the prophylaxis schedule according to the recommendations. Based on WHO criteria, 31 children (19.3%) developed severe malaria; most of the patients with severe malaria were VFR travelers and were significantly younger, had higher leukocytosis, had more thrombocytopenia, higher CRP, and lower natremia compared with patients with an uncomplicated course. All children recovered fully.

Conclusions:
Malaria is a significant cause of morbidity among returning travelers and newly arrived immigrants to Belgium. Most of the children had an uncomplicated disease course. Physicians should educate families about traveling to malaria-endemic areas to correct malaria preventive measures and prophylaxis.