bluuebunny/crossref_metadata_2025_split huggingface dataset:
Moderating effects of uric acid and sex on cognition and psychiatric symptoms in asymmetric Parkinson's disease
Authors: Constantin Ioana Medeleine , Voruz Philippe , Péron Julie Anne 
Published: April 2023

Abstract

Background. Non-motor symptoms are an important early feature of Parkinson’s disease (PD), encompassing a variety of cognitive and psychiatric symptoms that seem to manifest differently depending on motor symptom asymmetry. Different factors, such as uric acid (UA) and sex, seem to influence cognitive and psychiatric expression in PD, however their interplay remains to be better understood.
Methods. Participants taking part in the Parkinson’s Progression Marker Initiative were studied based on the side of motor-symptom asymmetry and sex. Three-way interaction modeling was used to examine the moderating effects of sex and UA on cognitive functions and psychiatric symptoms.
Results. Significant three-way interactions were highlighted at one-year follow-upbetween motor symptom asymmetry, UA and sex for immediate and long-term memory in female patients exhibiting predominantly left-sided motor symptoms, and for processing speed and sleepiness in female patients exhibiting predominantly right-sided motor symptoms. No significant interactions were observed for male patients. Moreover, female patients exhibiting predominantly right-sided motor symptoms demonstrated lower serum UA concentrations and had overall better outcomes, while male patients with predominantly right-sided motor symptoms demonstrated particularly poor outcomes.
Conclusions. These findings suggest that in the earliest stages of the disease, UA and sex moderate cognitive functions and psychiatric symptomsdifferently depending on motor asymmetry, holding important clinical implications for symptom management in patients.
Differentiations of neonatal mice spermatogonial stem cells in presence of different concentration of alpha-Ketoglutarate
Authors: Jahanbakhsh Mahdi , Mirzapour Tooba , Asgari Fatemeh , Fadakar Hediyeh , Koruji Morteza 
Published: April 2023

Abstract
For the first time, alpha-ketoglutarate (AKG) was used to differentiate Spermatogonial stem cells (SSCs) in the presence of BMP-4 and Retinoic acid. So, SSCs were isolated from testis of 3-6 day-old mice by enzymatic digestion by collagenase and trypsin. The Cell suspension was cultured for one week in DMEM/F12 and 20% FBS in presence of GDNF growth factor. The proliferated cells were divided between control and treatment groups. In the control group, the cells were cultured for three weeks in DMEM/F12 containing 10% FBS in presence of 10-6 M retinoic acid and 40 ng/ml of BMP-4. A dose of 0.1 M AKG was added to the treatment group. The presence of Sertoli cells in culture system was confirmed by positive reaction of vimentin immunocytochemistry. The colonies that appeared on Sertoli cells also showed positive alkaline phosphatase activity and Oct4 immunocytochemistry reaction. qRT-PCR studies showed that the expression of Acrosin and Sycp3 genes was low in two groups after 7 days of culture. 21 days after culture, in the treatment group, the expression of Acrosin and Sycp3 genes was significantly increased rather than control group (p≤0.05). Large number of early spermatids were observed in the treatment group based on TEM studies.
Don’t shoot! How poaching may affect richness, biomass, and occupancy of non-volant mammals in an Atlantic Forest protected area, Brazil
Authors: Ferreguetti Atilla , Allemand Marina M. , Pereira-Ribeiro Juliane , Tomas Walfrido M. , Rocha Carlos F. D. , Bergallo Helena G. , Miller David A. W. 
Published: April 2023

Abstract

Hunting in tropical forests provides nutrition and financial income for local communities and is deeply rooted in social and cultural traditions. On the other hand, this activity, by locally reducing or extirpating populations of game species, may be responsible for the degradation and loss of key forest maintenance services. In Brazil, hunting is illegal (hereafter referred to as poaching). We evaluated, by accounting for imperfect detectability, the effects caused by poaching on mammals in the Vale Natural Reserve. We collected data over 5-years (between 2013 to 2018) using line-transect and camera-trapping to evaluate poaching effects on the density, biomass, richness, and site occupancy of mammalian species. Our complete data set included a total survey effort of 32,175 camera days across five camera trap surveys (825 working days), with a total of 25,254 records of 27 non-volant mammals. We found a negative effect of poaching index on overall-species richness based on our multi-species occupancy model. The greatest reduction in biomass occurred for ungulates and armadillos. Our results suggest that poaching can have large consequences for biodiversity in one of Brazil's largest Atlantic Forest remnants. Ours is the first study in the Atlantic Forest estimating the medium-term poaching impact on mammalian populations, demonstrating a potentially negative effect on density, biomass, and occupancy of species which are of importance for local ecosystem functioning. We demonstrate the need to start monitoring other populations of vertebrates that are a target to poaching, not only in the Atlantic Forest but in other Neotropical ecosystems.
Enlarged Perivascular Spaces and Swallow Dysfunction after Acute Ischemic Stroke
Authors: Wang Liu-Yuan , Hu Jing-Jie , He Jin-Cai , Huang Gui-Qian 
Published: April 2023

Abstract
Background Post-stroke dysphagia (PSD) is a severe complication of stroke. Cerebral small-vessel disease (CSVD) is a major risk factor for stroke and is associated with swallowing difficulties. Accumulating evidence suggests that enlarged perivascular spaces (ePVSs) are a feature of CSVD. We explored whether ePVSs affect swallowing function in patients with acute ischemic stroke.Methods This was a retrospective analysis of 395 patients with acute ischemic stroke who underwent swallowing examination after stroke who had a cerebral MRI at baseline. Swallowing function was ranked based on Functional Oral Intake Scale (FOIS) score, and ePVSs were rated using a 4-level severity score according to the number of ePVSs in the basal ganglia (BG-ePVS).Results Post-stroke dysphagia was detected in 57.4% of the patients and was related to the presence of BG-ePVS. The degree of ePVS was associated with a strong increase in the risk for incident dysphagia, independent of other standard risk factors for dysphagia. A Spearman correlation analysis showed that an increased FOIS score correlated positively with an increase in the BGPS grade.Conclusions: BG-ePVS are associated with swallowing dysfunction in patients with acute ischemic stroke. The larger the number of BG-ePVS, the more severe the post-stroke dysfunction in swallowing. Accordingly, ePVS should be considered a factor in the prediction of PSD.
Evolutionary and immune microenvironment dynamics during neoadjuvant treatment of oesophagael adenocarcinoma
Authors: Barroux Melissa , Househam Jacob , Lakatos Eszter , Ronel Tahel , Baker Ann-Marie , Salié Henrike , Mossner Max , Smith Kane , Kimberley Chris , Nowinski Salpie , Berner Alison , Gunasri Vinaya , Jansen Marnix , Caravagna Giulio , Steiger Katja , Slotta-Huspenina Julia , Weichert Wilko , Alberstmeier Markus , Chain Benny , Friess Helmut , Bengsch Bertram , Schmid Roland , Siveke Jens , Quante Michael , Graham Trevor 
Published: April 2023

Abstract
Locally advanced oesophageal adenocarcinoma (EAC) remains difficult to treat because of common resistance to neoadjuvant therapy and high recurrence rates. The ecological and evolutionary dynamics responsible for treatment failure are incompletely understood. Here, we performed a comprehensive multi-omic analysis of samples collected from EAC patients in the MEMORI clinical trial, revealing major changes in gene expression profiles and immune microenvironment composition that did not appear to be driven by changes in clonal composition. Multi-region multi-timepoint whole exome (300x depth) and paired transcriptome sequencing was performed on 27 patients pre-, during and after neoadjuvant treatment. EAC showed major transcriptomic changes during treatment with upregulation of immune and stromal pathways and oncogenic pathways such as KRAS, Hedgehog and WNT. However, genetic data revealed that clonal sweeps were rare, suggesting that gene expression changes were not clonally driven. Additional longitudinal image mass cytometry was performed in a subset of 15 patients and T-cell receptor sequencing in 10 patients, revealing remodelling of the T-cell compartment during treatment and other shifts in microenvironment composition. The presence of immune escape mechanisms and a lack of clonal T-cell expansions were linked to poor clinical treatment response. This study identifies profound transcriptional changes during treatment with limited evidence that clonal replacement is the cause, suggesting phenotypic plasticity and immune dynamics as mechanisms for therapy resistance with pharmacological relevance.
Investigation on the possibility for production of immune molecules by rat brain and pancreatic cells in their role as participants in regulatory pathways
Authors: Sainova Iskra Ventseslavova , Kolyovska Vera , Ilieva Iliana , Drenska Desislava , Maslarov Dimitar , Dimitrova-Dikanarova Dimitrina , Hadjiolova Radka , Markova Tzvetanka 
Published: April 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Assessing ICD Data Quality and Its Impact on DRG Payments: Evidence from a Chinese Hospital
Authors: Zhang Ying , Han Dong , Lyu Chen , Jiang Xian-han , Wei Lingyun 
Published: April 2023

Abstract
Background The International Statistical Classification of Diseases and Related Health Problems (ICD) codes play a critical role as fundamental data for hospital management and can significantly impact Diagnosis-Related Groups (DRGs). This study investigated the quality issues associated with ICD data and their impact on improper DRG payments.Methods Our study analyzed data from a Chinese hospital between 2016 and 2017 to evaluate the impact of ICD data quality on CN-DRG evaluation variables and payments. We assessed different stages of the ICD generation process and established a standardized process for evaluating ICD data quality and relevant indicators. The validation of the Data Quality Assessment (DQA) was confirmed through sampling data.Results This study of 85,522 inpatient charts found that gynecology had the highest and obstetrics had the lowest diagnosis agreement rates. Pediatrics had the highest agreement rates for MDC and DRG, while neonatal pediatrics had the lowest. The CMI of Coder- showed to be more reasonable than physician-, with increased DRG payments in obstetrics and gynecology. The DQA model revealed coding errors ranging from 40.32–65.18% for physician and 12.29–23.65% for coder. Payment discrepancies were observed, with physicians resulting in underpayment and coders displaying overpayment in some cases.Conclusion ICD data is crucial for effective healthcare management, and implementing standardized and automated processes to assess ICD data quality can improve data accuracy. This enhances the ability to make reasonable DRG payments and accurately reflects the quality of healthcare management.
Comprehensive rehabilitation and job reintegration of people with severe mental illness in a Latin American country: REINTEGRA pilot study
Authors: Carmona-Huerta Jaime , Durand-Arias Sol , Cárdenas-García Elsy , Arámbula-Román J. Carlos , Guzmán-Ramírez Marianne , Estrada-Ramírez Isabel , Amezcua-Ramírez María Trinidad , Lastra-González Verónica , Castiello-de-Obeso Santiago , Aldana-López Alejandro 
Published: April 2023

Abstract

Background
Despite the increasing use of comprehensive rehabilitation models for people with severe mental illness (SMI), there are still limitations to their implementation and replicability in a consensual way, particularly in Latin American countries. The REINTEGRA program aims to be a standardized model of comprehensive rehabilitation focused on psychosocial and cognitive improvement through a set of interventions on different areas of people’s functionality, with the goal of reintegrating people with SMI into the labour market. In this paper we summarize the protocol for its subsequent implementation in a mental health institution in Mexico.
Method
The protocol is based on a quasi-experimental, prospective longitudinal study, with a pragmatic or naturalistic control group. It will be carried out in three phases. Phase 1 consists of a series of interventions focused on psychosocial improvement; Phase 2 focuses on cognitive and behavioral improvement treatments; and Phase 3 targets psychosocial recovery through rehabilitation and reintegration into the labour market. The overall procedure will be monitored with standarized evaluations at different stages of the program.
Discussion
This study presents a model of integral rehabilitation of people with SMI. At the moment, one of the obstacles to overcome is the organization and procedural control of the different actors needed for its implementation (nurses, psychologists, doctors, companies, institutions, etc.). REINTEGRA will be the first comprehensive rehabilitation model that includes systematized procedures for job reinsertion for people with SMI in Mexico, which aims to be a standardized tool of easy adaptation and the replicability for other mental health centers and institutions.
Research Ethics Committee Approval: 205-2021.
Cerebrospinal fluid viral etiology of patients with suspected central nervous system infections during the COVID-19 outbreak
Authors: Aliabadi Nasrin , Marandi Nahid Heydari , Hamzavi Seyedeh Sedigheh , Pouladfar Gholamreza , Hosseini Marzieh , Bahrololoom Rosemina , Jamalidoust marzieh , Ziyaeyan Mazyar 
Published: April 2023

Abstract
Purpose: Although viral etiology of central nervous system (CNS) infections such as meningitis and encephalitis are investigated widely worldwide, it remains to be declared under the COVID-19 pandemic. In this study, we aimed to investigate the etiology of viral CNS infections in patients hospitalized in a single referral hospital during the outbreak in Iran.
Methods: We retrospectively collected the CSF samples and reviewed the medical records of patients hospitalized with suspected viral CNS infection in Shiraz, a large city in southern Iran, from April 2021 to May 2022. One hundred sixty-six cerebrospinal fluid (CSF) samples were tested primarily for detection of HSV 1 and 2 and were retrospectively analyzed by primers PCR targeted to coronavirus 2 (SARS-COV-2), human herpesviruses 1 to 6 (HHV-1 to -6), polyomaviruses JC, and enteroviruses (EV).
Results: Of the total CSF samples analyzed by PCR, 22 (13.3%) were positive for only one viral pathogen, and just one (0.6%) sample was detected with multiple viruses (HSV-1 and CMV). The SARS-COV-2 (n=6) was the second most common viral etiology just after Herpes simplex virus (HSV-1) 6.6% (11 cases). Other detected viruses were CMV for 1.8% (3), VZV for 1.2 % (2), and JC for 0.6% (1) of the cases.
Conclusion: According to the above, the spread of SARS-COV-2 during the COVID-19 pandemic, alongside HSV-1, CMV, and VZV in Iran, was significant. As a result, it can play an essential role in development of CNS-related diseases, which required urgent diagnosis and treatment.
Numerical simulation of progressive failure law of thick-hard rock strata in the upper coal seam
Authors: Xu Chao , Zhao Chunyu , Wang Kai , Sun Haoshi , Wang Yifu 
Published: April 2023

Abstract

The thick-hard rock strata (THRS) are widely distributed among coal-bearing strata. In the process of coal mining, the strong dynamic load will be produced after the THRS are broken, which will lead to dynamic disasters such as mine earthquake, rock burst and so on. Therefore, revealing the breaking law of THRS is helpful to prevent and control the dynamic disasters. This paper constructed the mechanical model of THRS based on thick plate theory and obtained the breaking distance of THRS, which is applied to Yangliu Coal Mine. And we constructed the numerical stratigraphic model and analyzed the caving characteristics, the changing rules of displacement and stress of THRS under different advancing distances. Then the above results are verified by the change of hydraulic support resistance. The research shows that the critical instability distance of THRS overlying the coal seam #10 in Yangliu Coal Mine was 296.4 m. Based on the numerical simulation, the displacement and stress of the main THRS increased greatly at the position of 250-300 m, and occurred a large-scale collapse. The hydraulic support resistance of the working face increased sharply and microseismic energy is released rapidly when the mining reached about 330 m, the reliability of the theoretical analysis and numerical simulation were verified by the engineering tests.
Expression profile, prognostic values, and immune infiltration of IRX family members in lung adenocarcinoma
Authors: Wang Feng , Zhang Xinglu , Li Wan , Zhou Lefei , Li Dongbing , Wang Dongliang 
Published: April 2023

Abstract
Background
The iroquois homologous homeobox (IRX) gene family may be involved in the development of a variety of tumors. However, comprehensive analysis of IRX family members in lung adenocarcinoma (LUAD) has rarely been reported.
Methods
From the Cancer Genome Atlas (TCGA), LUAD samples were extracted. The roles of IRXs were comprehensively analyzed using Kaplan–Meier Plotter, cBioPortal, and R software (version 3.6.3).
Results
The expression of IRX1/2/3/6 was significantly lower in LUAD compared to normal lung tissue, while the expression of IRX4 was significantly higher in LUAD compared to normal lung tissue. The expression of IRX was associated with T stage, number_pack_years_smoked, N stage, gender, primary treatment outcome, and smokers. In LUAD, IRX2 downregulation was an independent factor that contributes to poor prognosis. Expression of multiple IRX genes showed some diagnostic biomarker values for LUAD. IRX genes were key players mediating the development and progression of LUAD through multiple pathways, including ras signaling pathway, glycosphingolipid biosynthesis-ganglio series, inositol phosphate metabolism, metabolic pathways, and pertussis. There was a significant association between immune infiltration and IRX genes.
Conclusions
The IRX family may represent novel prognostic biomarkers, as well as immunotherapeutic targets for LUAD.
Comparison of dance and gymnastic intervention on cognition and neuropsychiatric symptoms in sedentary elderly with cognitive impairment
Authors: AYARI Sawsen , Abellard Alexandre , SAKRANI Sihem , Krinitskaia Anastasia , Grzelak Marta , Bounader Raymond , Gavarry Olivier 
Published: April 2023

Abstract
Background: To compare the effect of dance and gymnastic intervention on cognition and neuropsychiatric symptoms in elderly with cognitive impairment.
Methods Twenty-three older adults (64-90 years-old), attending the day care center and diagnosed with cognitive impairment, were randomized to a 16-week dance intervention or a gymnastic intervention (60 min/week). Cognitive function (MMSE), neuropsychiatric symptoms (GDS-15, NPI), and physical function (TUG, ADL) were evaluated at the baseline and at the end of the intervention. After estimation by the Borg scale, these two physical activities were performed at similar intensities (60-70% HRR).
Results MMSE score was significantly increased after the intervention in dance group (+3.3/+14%, p=0.03), especially memory recall (+1/+220 %, p=0.03), but not for the gymnastic group. GDS-15 and NPI decreased significantly (p<0.001) after the intervention in both groups However, no significant effect was noted for TUG and ADL.
Conclusion The study findings support the potential utility of dance as a multimodal intervention for improving cognition. A low frequency ecological dance intervention (1time/week; 60 min) is sufficient to enhance cognition. In further practise, dance exercise could be more practised in community-dwelling.
Effect of cellulose-based fillers on vulcanized natural rubber
Authors: Freitas Flavia Leticia Silva , Correia Carla Almêda , de Oliveira Letícia Mota , Ribeiro Hélio , Valera Ticiane Sanches 
Published: April 2023

Abstract
Cellulose nanocrystal (CNC) fillers have gained attention in research and industrial applications owing to their excellent properties and environmental bias. They can be added to natural rubber (NR) compounds to enhance properties such as the modulus of elasticity. CNCs can be extracted by different acid methods that promote singular features in interfacial adhesion, according to the type of acid used. This study addresses the feasibility of using cellulose nanoparticles in vulcanized NR composites. For this purpose, two different hydrolysis procedures using sulfuric acid and the less aggressive phosphoric acid were performed. These nanoparticles were then added to different amounts of NR compound. The effects of the CNCs on the vulcanization of the NR compound were evidenced by the formation of a zinc-cellulose-rubber complex, which reduced the optimal vulcanization time and increased the NR compound cure rate, particularly for the samples treated with phosphoric acid. In addition, the formation of this complex structure affected the morphology and mechanical properties of the composites. In particular, the tensile strength, elongation at break, and modulus at 300% of the composites with nanocellulose treated with phosphoric acid increased by 90%, 16%, and 51%, respectively, compared to the NR compound. Furthermore, the higher aspect ratio of the nanocellulose particles, mainly treated with phosphoric acid, favored the filler-matrix adhesion, making them a promising alternative to enhance the mechanical properties of NR compounds.
Ground ice-magma interaction resurfaced the landing region of the Zhurong rover during the Amazonian
Authors: Michalski Joseph , Liu Jiacheng , Wu Bo , Wu Xing , Liu Yang , Gao Wenyuan , Qian Yuqi , Ling Zongcheng , Wu Zhongchen , Liu Changqing , Williams-Jones Anthony , Zhao Jiannan , Zhou Mei-Fu , Hu Sen , Sun Yu , Lin Honglei , Tan Wei 
Published: April 2023

AbstractDespite a consensus view that Mars has been cold and icy throughout the Amazonian (3 billion years ago until present), the Zhurong rover has provided striking evidence for aqueous resurfacing processes in and around the landing site during this time period. Here, we report a close association of magmatism (i.e.dikes) and several distinctive landforms (e.g.mud volcanoes and troughs) indicating that ground ice in the Vastitas Borealis Formation (VBF) was thawed by the magma heating causing the collapse of VBF to form distinct landforms and alteration mineralogy. This collapse released pressurized meltwater and mud, producing mud volcanoes and muddy water. Upon freezing, the slurry materials were further buried by younger porous sediments. Freezing of the upwelled chemical-rich groundwater potentially circulated by cryosuction produced large crystals of sulphate (probably selentite) unlike sulphates previously identified on Mars, evidencing relatively young aqueous alteration on Mars driven by magma-ice interactions.