bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet levels and age are determinants of survival after mild–moderate TBI: A prospective study in Spain
Authors: Yuguero Oriol , Vena Ana , Bernal Maria , Martínez-Alonso Montserrat , Farre Joan , Purroy Francisco 
Published: March 2023

IntroductionTraumatic brain injury (TBI) is a very important reason for consultation in emergency departments.MethodsA hospital cohort study with patients who attended a hospital emergency department between June 1, 2018 and December 31, 2020 due to TBI was studied. Clinical and sociodemographic variables were recorded. The levels of biomarkers and management variables were used. Qualitative variables were analyzed using Pearson's chi-square test, and quantitative variables using the Mann–Whitney U-test. Survival analyses were performed by fitting a multivariable Cox regression model for patient survival during the follow-up of the study in relation to the patient's characteristics upon admission to the emergency department.ResultsA total of 540 patients were included. The mean age was 83 years, and 53.9% of the patients were men. Overall, 112 patients (20.7%) died during the study follow-up. The mortality rate per 100 person-years was 14.33 (11.8–17.24), the most frequent mechanism being falls in the home, with none caused on public roads. The multivariable Cox proportional hazards model showed that survival after TBI was significantly associated with age, S100 levels, Charlson index, patient's institutionalized status, the place where the TBI occurred, and hemoglobin and platelet levels.DiscussionThe most common profile for a patient with a TBI was male and aged between 80 and 90 years. The combination of the variables age, Charlson index, place of TBI occurrence, and hemoglobin and platelet levels could offer early prediction of survival in our population independently of TBI severity. With the data obtained, a therapeutic algorithm could be established for patients suffering from mild TBI, allowing the patient to be supervised at home, avoiding futile referrals to emergency services.
Resource-use efficiency and environmental sustainability in the village tank cascade systems in the dry zone of Sri Lanka: An assessment using a bio-economic model
Authors: Dayananda Dasuni , Weerahewa Jeevika , Weerasooriya Senal A. 
Published: March 2023

IntroductionVillage tank cascade systems (VTCSs) were built in ancient Sri Lanka as autonomous and climate-resilient agro-ecological systems. This study examines crop choices, farming profitability, and environmental sustainability under alternative rainfall regimes and market interventions in the Mahakanumulla VTCS of the Anuradhapura district.MethodA bio-economic model was developed to represent farming activities in the VTCS for the 2018-19Mahaand 2019Yalacultivation seasons with data gathered from secondary sources and a key informant survey. The objective function of the model was the maximization of profits from farming. Resource limits were set for four types of land (highlands and lowlands in theMahaandYalaseasons), two types of labor (hired and family), and twelve-monthly water constraints. Six different models were developed for the six sub-divisions of the VTCS, considering the water-management hierarchy of the system. The models were simulated under alternative rainfall regimes and market interventions. The optimal crop mixes, farm profits, and shadow prices of resources associated with the baseline scenarios were compared with those of the counterfactual scenarios.Results and discussionThis analysis clearly illustrated that water and labor are the key determinants of the system. Also, when 922 ha of lowlands and 205 ha of uplands were allocated per annum for crop cultivation under normal environmental conditions, the annual profitability of the VTCS was LKR 111 million. During drought periods, a sharp reduction in profits was observed in theMahaseason. Year-round drought caused a 77% profit reduction compared to the baseline. The Maha drought alone caused a reduction of 47%. The introduction of a buy-back arrangement for chili and maize helped farmers to increase profits by 185 and 28%, respectively, under normal climate scenarios, turning to 954 and 5% during extreme drought scenarios, compared to the baseline. The least nitrate leaching and soil losses occurred in green chili cultivation. The introduction of market-based solutions is recommended to address extreme climate events experienced by the rural communities dependent on the VTCSs in Sri Lanka.
The learning curve of endoscopic endonasal transsphenoidal surgery for pituitary adenomas with different surgical complexity
Authors: Huang Jinxiang , Hong Xinjie , Cai Zheng , Lv Qian , Jiang Ying , Dai Wei , Hu Guohan , Yan Yong , Chen Juxiang , Ding Xuehua 
Published: March 2023

ObjectiveTo investigate the learning curve under different surgical complexity in endoscopic transsphenoidal approach for pituitary adenoma.Methods273 patients undergoing endoscopic transsphenoidal surgery for pituitary adenoma were collected retrospectively and divided into three groups chronologically (early, middle, and late periods). Surgical complexity was differentiated based on Knosp classification (Knsop grade 0–2 vs. Knosp grade 3–4), tumor maximum diameter (MD) (macroadenomas vs. giant adenomas), and history of previous surgery for pituitary adenoma (first operation vs. reoperation). Then the temporal trends in operative time, surgical outcomes, and postoperative complications were evaluated from early to late.ResultsThe median operative time decrease from 169 to 147 min across the three periods (P = 0.001). A significant decrease in operative time was seen in the simple groups [Knosp grade 0–2 adenoma (169 to 137 min, P &lt; 0.001), macroadenoma (166 to 140 min, P &lt; 0.001), and first operation (170.5 to 134 min, P &lt; 0.001)] but not in their complex counterparts (P &gt; 0.05). The GTR rate increased from 51.6% to 69.2% (P = 0.04). The surgical period was an independent factor for GTR in the simple groups [Knosp grade 0–2 adenoma: OR 2.076 (95%CI 1.118–3.858, P = 0.021); macroadenoma: OR = 2.090 (95%CI 1.287–3.393, P = 0.003); first operation: OR = 1.809 (95%CI 1.104–2.966, P = 0.019)] but not in the complex groups. The biochemical cure rate increased over periods without statistical significance (from 37.5% to 56.3%, P = 0.181). Although intraoperative CSF leakage rose (from 20.9% to 35.2%) and postoperative CSF leakage reduced (from 12.1% to 5.5%), there was no statistically significant trend across the three time periods (P &gt; 0.05).ConclusionThis study showed that complex operations might have a prolonged learning curve. Differentiating surgical difficulty and using multivariate combined analysis may be more helpful in clinical practice.
Field epidemiology capacity of the national veterinary services of Lao PDR: An online survey
Authors: Subharat Supatsak , Meunsene Dethaloun , Putthana Vannaphone , Tiwari Harish , Firestone Simon M. 
Published: March 2023

IntroductionCapacity in veterinary epidemiology is critical to detect, respond and control infectious diseases. Laos veterinary service is limited by having only a small number of veterinarians who graduated from overseas. Animal science graduates support the majority of the Laos veterinary services. The veterinary program was established in 2009 at the National University of Laos. We aimed to understand the national veterinary epidemiology capacity and identify gaps and training needs.MethodA cross-sectional online study was conducted in 2021 targeting central (DLF), provincial (PAFO) and district (DAFO) government animal health officers and veterinary/animal science academics (n = 332). The questionnaire addressed skills, experiences and perceived training needs in outbreak investigation, disease surveillance, data management and analysis, epidemiological surveys, One Health, leadership and communication and biosecurity. A descriptive analysis was performed and associations between demographic factors and epidemiological skills were examined.Results and discussionIn total, 205 respondents completed the questionnaire (61.8% response rate). Respondents reported low or no skills and experience in data management and analysis, epidemiological surveys and One Health. In contrast, higher but limited skills and experiences were reported in outbreak investigation, disease surveillance and biosecurity. Previous epidemiology training was primarily associated with stronger experiences in veterinary epidemiology competencies, followed by respondents that had completed a veterinary degree, highlighting the value of the currently available epidemiology training and veterinary-trained personnel in Lao PDR. This study could help inform the Laos government in its policy planning for field veterinary epidemiology capacity and future training.
Genetic Diversity in Oilseed and Vegetable Mustard (Brassica juncea L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers
Authors: Zhang Dongsuo , Yu Haibo , Gao Lianliang , Wang Jing , Dong Hui , Guo Yuan , Hu Shengwu 
Published: March 2023

Genetic diversity analysis is a fundamental work for effective management and utilization of plant germplasm. Brassica juncea is an economically important crop, including both oilseed and vegetable types. In the present study, a total of 99 accessions of Brassicaceae family, including 84 mustard (50 oilseed and 34 vegetable types) in China and 15 other Brassicaceae accessions were evaluated for their genetic diversity using nuclear and mitochondrial molecular markers. All accessions were evaluated using 18 simple sequence repeats, 20 sequence related amplified polymorphisms, and 7 intron-exon splice junction primers, and in total, 232 polymorphic fragments were obtained. The unweighted pair-group method with arithmetic mean cluster analysis indicated that all accessions could be divided into three major clusters, with cluster I including all 50 oilseed mustard, cluster II including 34 vegetable mustard, and cluster III containing 15 other Brassicaceae accessions. The results of principal component analysis and population structure analysis were in accordance with the cluster result. Molecular variance analysis revealed that the genetic variation was 34.07% among populations and 65.93% within Brassica species, which indicates existence of considerable genetic variation among oilseed and vegetable B. juncea species. Based on an InDel and a SNP locus reported in B. juncea mitochondrial genome, all the 84 B. juncea mitochondrial genomes were divided into three mitotypes (MTs1-3), 22 accessions of MT1, 20 accessions of MT2, and 42 accessions of MT3. In addition, the results of the modified multiplex PCR, Indel and SNP could identify pol-, cam-, nap- (or MT4), Bol-, Bni-, Esa-, and In-cytoplasmic types in 15 other Brassicaceae accessions. Together, oilseed and vegetable B. juncea can be used for broadening the genetic background for each other.
Genome-Wide Identification and Expression Analysis of Rosa roxburghii Autophagy-Related Genes in Response to Top-Rot Disease
Authors: Luo Kaisha , Li Jiaohong , Lu Min , An Huaming , Wu Xiaomao 
Published: March 2023

Autophagy is a highly conserved process in eukaryotes that degrades and recycles damaged cells in plants and is involved in plant growth, development, senescence, and resistance to external stress. Top-rot disease (TRD) in Rosa roxburghii fruits caused by Colletotrichum fructicola often leads to huge yield losses. However, little information is available about the autophagy underlying the defense response to TRD. Here, we identified a total of 40 R. roxburghii autophagy-related genes (RrATGs), which were highly homologous to Arabidopsis thaliana ATGs. Transcriptomic data show that RrATGs were involved in the development and ripening processes of R. roxburghii fruits. Gene expression patterns in fruits with different degrees of TRD occurrence suggest that several members of the RrATGs family responded to TRD, of which RrATG18e was significantly up-regulated at the initial infection stage of C. fructicola. Furthermore, exogenous calcium (Ca2+) significantly promoted the mRNA accumulation of RrATG18e and fruit resistance to TRD, suggesting that this gene might be involved in the calcium-mediated TRD defense response. This study provided a better understanding of R. roxburghii autophagy-related genes and their potential roles in disease resistance.
Three-Dimensional Voxel-Wise Quantitative Assessment of Imaging Features in Hepatocellular Carcinoma
Authors: Huang Chongfei , Ying Shihong , Huang Meixiang , Qiu Chenhui , Lu Fang , Peng Zhiyi , Kong Dexing 
Published: March 2023

Voxel-wise quantitative assessment of typical characteristics in three-dimensional (3D) multiphase computed tomography (CT) imaging, especially arterial phase hyperenhancement (APHE) and subsequent washout (WO), is crucial for the diagnosis and therapy of hepatocellular carcinoma (HCC). However, this process is still missing in practice. Radiologists often visually estimate these features, which limit the diagnostic accuracy due to subjective interpretation and qualitative assessment. Quantitative assessment is one of the solutions to this problem. However, performing voxel-wise assessment in 3D is difficult due to the misalignments between images caused by respiratory and other physiological motions. In this paper, based on the Liver Imaging Reporting and Data System (v2018), we propose a registration-based quantitative model for the 3D voxel-wise assessment of image characteristics through multiple CT imaging phases. Specifically, we selected three phases from sequential CT imaging phases, i.e., pre-contrast phase (Pre), arterial phase (AP), delayed phase (DP), and then registered Pre and DP images to the AP image to extract and assess the major imaging characteristics. An iterative reweighted local cross-correlation was applied in the proposed registration model to construct the fidelity term for comparison of intensity features across different imaging phases, which is challenging due to their distinct intensity appearance. Experiments on clinical dataset showed that the means of dice similarity coefficient of liver were 98.6% and 98.1%, those of surface distance were 0.38 and 0.54 mm, and those of Hausdorff distance were 4.34 and 6.16 mm, indicating that quantitative estimation can be accomplished with high accuracy. For the classification of APHE, the result obtained by our method was consistent with those acquired by experts. For the WO, the effectiveness of the model was verified in terms of WO volume ratio.
Characterization and Application of the Sugar Transporter Zmo0293 from Zymomonas mobilis
Authors: Zhang Kun , Zhang Wenwen , Qin Mengxing , Li Yi , Wang Hailei 
Published: March 2023

Zymomonas mobilis is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for the commercial production of desirable bioproducts. Sugar transporters are responsible for the import of substrate sugars and the conversion of ethanol and other products. Glucose-facilitated diffusion protein Glf is responsible for facilitating the diffusion of glucose uptake in Z. mobilis. However, another sugar transporter-encoded gene, ZMO0293, is poorly characterized. We employed gene deletion and heterologous expression mediated by the CRISPR/Cas method to investigate the role of ZMO0293. The results showed that deletion of the ZMO0293 gene slowed growth and reduced ethanol production and the activities of key enzymes involved in glucose metabolism in the presence of high concentrations of glucose. Moreover, ZMO0293 deletion caused different transcriptional changes in some genes of the Entner Doudoroff (ED) pathway in the ZM4-ΔZM0293 strain but not in ZM4 cells. The integrated expression of ZMO0293 restored the growth of the glucose uptake-defective strain Escherichia coli BL21(DE3)-ΔptsG. This study reveals the function of the ZMO0293 gene in Z. mobilis in response to high concentrations of glucose and provides a new biological part for synthetic biology.
Starvation Induces Extracellular Accumulation of Polyphosphate in Dictyostelium discoideum to Inhibit Macropinocytosis, Phagocytosis, and Exocytosis
Authors: Rijal Ramesh , Ismail Issam , Jing Shiyu , Gomer Richard H. 
Published: March 2023

Dictyostelium discoideum is a soil-dwelling unicellular eukaryote that accumulates extracellular polyphosphate (polyP). At high cell densities, when the cells are about to overgrow their food supply and starve, the corresponding high extracellular concentrations of polyP allow the cells to preemptively anticipate starvation, inhibit proliferation, and prime themselves to begin development. In this report, we show that starved D. discoideum cells accumulate cell surface and extracellular polyP. Starvation reduces macropinocytosis, exocytosis, and phagocytosis, and we find that these effects require the G protein-coupled polyP receptor (GrlD) and two enzymes, Polyphosphate kinase 1 (Ppk1), which is required for synthesizing intracellular polyP, cell surface polyP, and some of the extracellular polyP, and Inositol hexakisphosphate kinase (I6kA), which is required for cell surface polyP and polyP binding to cells, and some of the extracellular polyP. PolyP reduces membrane fluidity, and we find that starvation reduces membrane fluidity; this effect requires GrlD and Ppk1, but not I6kA. Together, these data suggest that in starved cells, extracellular polyP decreases membrane fluidity, possibly as a protective measure. In the starved cells, sensing polyP appears to decrease energy expenditure from ingestion, and decrease exocytosis, and to both decrease energy expenditures and retain nutrients.
Bioinspired Cyclic Dipeptide Functionalized Nanofibers for Thermal Sensing and Energy Harvesting
Authors: Santos Daniela , Baptista Rosa M. F. , Handa Adelino , Almeida Bernardo , Rodrigues Pedro V. , Torres Ana R. , Machado Ana , Belsley Michael , de Matos Gomes Etelvina 
Published: March 2023

Nanostructured dipeptide self-assemblies exhibiting quantum confinement are of great interest due to their potential applications in the field of materials science as optoelectronic materials for energy harvesting devices. Cyclic dipeptides are an emerging outstanding group of ring-shaped dipeptides, which, because of multiple interactions, self-assemble in supramolecular structures with different morphologies showing quantum confinement and photoluminescence. Chiral cyclic dipeptides may also display piezoelectricity and pyroelectricity properties with potential applications in new sources of nano energy. Among those, aromatic cyclo-dipeptides containing the amino acid tryptophan are wide-band gap semiconductors displaying the high mechanical rigidity, photoluminescence and piezoelectric properties to be used in power generation. In this work, we report the fabrication of hybrid systems based on chiral cyclo-dipeptide L-Tryptophan-L-Tryptophan incorporated into biopolymer electrospun fibers. The micro/nanofibers contain self-assembled nano-spheres embedded into the polymer matrix, are wide-band gap semiconductors with 4.0 eV band gap energy, and display blue photoluminescence as well as relevant piezoelectric and pyroelectric properties with coefficients as high as 57 CN−1 and 35×10−6 Cm−2K−1, respectively. Therefore, the fabricated hybrid mats are promising systems for future thermal sensing and energy harvesting applications.
Fiber Optic Temperature Sensor System Using Air-Filled Fabry–Pérot Cavity with Variable Pressure
Authors: Chowdhury Hasanur R. , Han Ming 
Published: March 2023

We report a high-resolution fiber optic temperature sensor system based on an air-filled Fabry–Pérot (FP) cavity, whose spectral fringes shift due to a precise pressure variation in the cavity. The absolute temperature can be deduced from the spectral shift and the pressure variation. For fabrication, a fused-silica tube is spliced with a single-mode fiber at one end and a side-hole fiber at the other to form the FP cavity. The pressure in the cavity can be changed by passing air through the side-hole fiber, causing the spectral shift. We analyzed the effect of sensor wavelength resolution and pressure fluctuation on the temperature measurement resolution. A computer-controlled pressure system and sensor interrogation system were developed with miniaturized instruments for the system operation. Experimental results show that the sensor had a high wavelength resolution (<0.2 pm) with minimal pressure fluctuation (~0.015 kPa), resulting in high-resolution (±0.32 ℃) temperature measurement. It shows good stability from the thermal cycle testing with the maximum testing temperature reaching 800 ℃.
Partial Replacement of Cationic Starch by Cationic Hardwood Kraft Lignin Does Not Compromise Key Paper Properties
Authors: Pinto Patrícia I. F. , Pinto Paula C. R. , Evtuguin Dmitry V. , Liebner Falk 
Published: March 2023

Competition issues with food industry have recently boosted the exploration of alternative solutions capable of replacing starch in papermaking to a certain extent. Covalent grafting of quaternary ammonium groups onto LignoBoost® Eucalyptus kraft lignin has recently shown promise in this regard as the cationic products feature excellent water solubility across the entire pH scale. Considering these suitable properties, cationic kraft lignin was applied and evaluated, for the first time, in the perspective of partial substitution of cationic starch in papermaking, using bleached Eucalyptus kraft pulp. Based on an assessment of key paper properties, such as mechanical (tensile, bursting, and tearing indexes), structural (roughness, capillarity rise, air resistance, internal strength, and water contact angle), and optical ones (brightness, opacity, and relative color change), it is safe to conclude that the partial replacement of conventional cationic starch by cationic lignin does not compromise these features. The results also show that properties can be fine-tuned by varying the degree of lignin derivatization, providing paper-specific solutions for replacing starch by lignin potentially available at large-scale at the pulp and paper industry, closing the loop within the circular economy concept.
Use of Soil Infiltration Capacity and Stream Flow Velocity to Estimate Physical Flood Vulnerability under Land-Use Change Scenarios
Authors: Hernández-Atencia Yelena , Peña Luis E. , Muñoz-Ramos Jader , Rojas Isabel , Álvarez Alexander 
Published: March 2023

Land-use changes produce variations in upper soil hydraulic properties and alter the hydrological response and hydraulic behavior of streams. Thus, the combined effect of variations in soil properties and current hydraulics interacts with the exposure of structures exposed and their degree of physical vulnerability. This study aims to evaluate the effect of land-use evolution from 1976 to 2017 on the physical vulnerability of structures exposed to floods in the Combeima cathment, Colombia, proposing two novel approaches: (i) based on soil infiltration capacity variation (CN) in the basin and changes in stream flow velocity (v), (ii) through soil water storage variation in the root zone (Hu). Hydrological and hydraulic modeling and the implementation of four physical vulnerability assessment methods were performed using GIS analysis. Findings indicate that simplifying physical vulnerability estimations through CN, Hu, and v variations in catchments and at cross-section resolutions is possible, allowing a detailed analysis of the land-use change effect on the vulnerability of structures. The scaling behavior of the physical vulnerability of structures was identified when Hu is defined as a scale variable and, similarly, concerning flow velocity in the stream. Therefore, applying the power law could be useful in planning processes with limited information.