bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Bioprinted Lipases in Microwave-Assisted Esterification Reactions
Authors: Carvalho de Melo Jéssica Jéssi , Passos da Silva Gardenia Laís , Mota Danyelle Andrade , de Souza Brandão Luma Mirely , de Souza Ranyere Lucena , Pereira Matheus M. , Lima Álvaro Silva , Soares Cleide Mara Faria 
Published: January 2023

In this study, a comparative evaluation was performed in batch esterification reactions under conventional heating (CH) and assisted by microwave irradiation (MW) using bioprinted lipases. Microwave-irradiation-assisted reactions generally provide higher productivities and improve synthesis performance in terms of increased rate and reduced reaction times, resulting in higher interest yields in less time. Productivity was calculated with the enzymes: Burkholderia cepacia lipase (BCL), Candida rugosa lipase (CRL), and porcine pancreas lipase (PPL) using different fatty acids (lauric acid (12:0), myristic acid (14:0), palmitic acid (16:0), stearic acid (18:0), and oleic acid (18:1)) and alcohols at a molar ratio of 1:8. The microwave reactor was operated at a temperature of 45 °C, and power varied between 50 W and 200 W. Bioprinted BCL (bBCL) showed the highest productivity among the tested lipases. In the reaction with the best result, bBCL with lauric acid under MW, the reaction time decreased from 24 h (CH) to 25 min (MW) and the productivity increased 33 times compared with the reactions under CH. The increase in productivity demonstrates its activation that occurred as a result of conformational changes of the enzyme in the bioprinting process, confirmed by Fourier transform infrared (FTIR) spectrometric analysis, which reduces the content of bBCL α-helix with lauric acid. The biocatalyst showed high operational stability over eight cycles, while losing only 19% of its initial activity with half-life times of 12.8 batches. The storage time was five weeks, maintaining ≈80% activity. The results demonstrate the prospect of a new enzymatic route to obtain hyperactive catalysts, with the use of bioprinted lipases in esterification reactions under microwave irradiation, for the synthesis of esters with a view to large-scale industrial application.
Transformation of Strawberry Plants’ Phenolic Profile after Treatment with a Mechanocomposite Based on Silicon Chelates in the Course of Development under In Vitro, Ex Vitro, and In Vivo Conditions
Authors: Kotsupiy Olga , Karpova Evgeniya , Trofimova Elena , Novikova Tatyana , Ambros Elena 
Published: January 2023

The positive effect of silicon on plants is thought to be mediated by a modification of phenolic metabolism. The purpose of the study was to evaluate the effect of a silicon-based mechanocomposite (MC) on alterations of the phenolic profile of strawberry plants in the course of development under in vitro, ex vitro, and in vivo conditions. Aqueous ethanol extracts of aboveground parts of in vitro–derived plants (Fragaria × ananassa cv. ‘Solnechnaya polyanka’) were subjected to HPLC. Nineteen individual phenolic compounds (hydroxybenzoic and hydroxycinnamic acids, catechins, ellagic acid derivatives, and flavonol glycosides) were quantified. The results revealed phenolic profiles specific to each studied stage and significant transformations of the profiles by the MC. It induced strong upregulation of hydroxycinnamic acid during in vitro rooting and of catechins and hydroxybenzoic acids during ex vitro acclimation. At ex vitro and in vivo stages, the emergence of quercetin glycosides and ellagitannins was registered, and the MC elevated their levels during ex vitro acclimation and field growth. Principal component analysis confirmed the significant effect of the MC on the phenolic profile at all stages, and this effect was the strongest during ex vitro acclimation. The results are consistent with previous reports on the modification of phenolic profiles of plants by silicon-derived biostimulants.
Investigation of the Relationship between Right Coronary Artery–Aorta Angle and Coronary Artery Disease and Associated Risk Factors
Authors: Geerlings-Batt Jade , Gupta Ashu , Sun Zhonghua 
Published: January 2023

At the level of the left coronary artery tree, there is evidence showing an association between bifurcation angle and coronary artery disease (CAD), and this motivated us to explore similar associations at the level of the right coronary artery (RCA). The purpose of this study was to determine whether there is a relationship between RCA–aorta angle and CAD and age, sex, body mass index, smoking status, hypertension, and high blood cholesterol. The coronary computed tomography angiography datasets and CAD risk factor checklists of 250 patients were retrospectively reviewed, with RCA–aorta angles measured via multiplanar reformation images. Independent t-tests were used to compare mean RCA–aorta angle measurements between groups, correlations between continuous variables were assessed using Pearson and Spearman correlations, and a general linear model was used to adjust for potentially confounding variables. Coronary angle measurements were conducted by two independent assessors with very strong intraclass correlation (r=0.999, p<0.001). A significantly smaller mean RCA–aorta angle was observed in the CAD group (79.07 ± 24.88°) compared to the normal group (92.08 ± 19.51°, p=0.001), in smokers (76.63 ± 22.94°) compared to non-smokers (85.25 ± 23.84°, p=0.016), and a narrow RCA–aorta angle was negatively correlated with BMI (r=−0.174, p=0.010). This study suggests a relationship between narrow RCA–aorta angles and CAD, smoking, and increasing BMI.
Trichoderma pubescens Elicit Induced Systemic Resistance in Tomato Challenged by Rhizoctonia solani
Authors: Behiry Said , Soliman Seham A. , Massoud Magdy A. , Abdelbary Moawad , Kordy Ahmed M. , Abdelkhalek Ahmed , Heflish Ahmed 
Published: January 2023

Rhizoctonia solani causes severe diseases in many plant species, particularly root rot in tomato plants. For the first time, Trichoderma pubescens effectively controls R. solani in vitro and in vivo. R. solani strain R11 was identified using the ITS region (OP456527); meanwhile, T. pubescens strain Tp21 was characterized by the ITS region (OP456528) and two genes (tef-1 and rpb2). The antagonistic dual culture method revealed that T. pubescens had a high activity of 76.93% in vitro. A substantial increase in root length, plant height, shoot fresh and dry, and root fresh and dry weight was indicated after applying T. pubescens to tomato plants in vivo. Additionally, it significantly increased the chlorophyll content and total phenolic compounds. The treatment with T. pubescens exhibited a low disease index (DI, 16.00%) without significant differences with Uniform® fungicide at a concentration of 1 ppm (14.67%), while the R. solani-infected plants showed a DI of 78.67%. At 15 days after inoculation, promising increases in the relative expression levels of three defense-related genes (PAL, CHS, and HQT) were observed in all T. pubescens treated plants compared with the non-treated plants. Plants treated with T. pubescens alone showed the highest expression value, with relative transcriptional levels of PAL, CHS, and HQT that were 2.72-, 4.44-, and 3.72-fold higher in comparison with control plants, respectively. The two treatments of T. pubescens exhibited increasing antioxidant enzyme production (POX, SOD, PPO, and CAT), while high MDA and H2O2 levels were observed in the infected plants. The HPLC results of the leaf extract showed a fluctuation in polyphenolic compound content. T. pubescens application alone or for treating plant pathogen infection showed elevated phenolic acids such as chlorogenic and coumaric acids. Therefore, the ability of T. pubescens to inhibit the growth of R. solani, enhance the development of tomato plants, and induce systemic resistance supports the application of T. pubescens as a potential bioagent for managing root rot disease and productivity increase of crops.
Large Prostate Volume Does Not Negatively Impact Health-Related Quality of Life in Patients with Prostate Cancer Treated with Ultrahypofractionated Stereotactic Body Radiotherapy
Authors: Milecki Piotr , Adamska Anna , Rucinska Anna , Pałucki Grzegorz , Szumiło Agnieszka , Skrobała Agnieszka , Jodda Agata , Michalak Michał 
Published: January 2023

Background: Survival outcomes after primary radiotherapy for localized prostate cancer (PCa) are excellent, regardless of the specific treatment modality. For this reason, health-related quality of life (HRQOL) has come to play an ever more important role in treatment selection. Stereotactic body radiation therapy (SBRT) is increasingly used to treat patients with PCa. However, the impact of prostate volume on HRQOL is not clear. In this study, we aimed to determine whether a large prostate volume negatively influences HRQOL outcomes in patients undergoing ultrahypofractionated SBRT. Material and Methods: We conducted a prospective study of 530 men with low- and intermediate-risk localized PCa. All patients were treated from 2013 to 2017 with SBRT (Cyberknife system). HRQOL data were collected at baseline (pre-treatment), immediately after treatment, and at 12 and 24 months. QOL variables were assessed with the European Organization for Research and Treatment of Cancer QLQ-C30 and PR-25 module. Differences in the QLQ-C30 scales were considered clinically relevant when the change was >10 points. For the analysis, patients were classified into two groups according to prostate volume (≤60 vs. >60 cm3). Results: The prostate volume was ≤60 cm3 in 415 patients (78.3%) and >60 cm3 in 115 (21.7%). No between-group differences were observed at baseline for any of the following variables: clinical stage; hormonal therapy; marital status; educational level; or employment status. No clinically-significant deterioration (functional and symptom scales) was observed in either group between the baseline and 24-month assessment. There were no clinically-relevant differences between the groups on any of the HRQOL variables, regardless of the prostate volume. Conclusions: This study shows that a large prostate volume (>60 cm3) does not appear to negatively impact HRQOL outcomes at two years in patients with localized prostate cancer treated with ultrahypofractionated SBRT administered with the CyberKnife system.
In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species
Authors: Randag Anna C. , de Kroon Lieke , Otten Henny , Arias Claro-Handgraaf Cindy , Schimmer Barbara , Kortbeek Titia , van Rooij Jeroen , Stelma Foekje F. 
Published: January 2023

Acanthamoeba keratitis is almost universally associated with contact lens (CL) use. Until today, however, CL solution manufacturing protocols lack testing of anti-amoebic activity. This study investigates the effectiveness of CL solutions available on the Dutch market against trophozoites and cysts of Acanthamoeba castellanii and Acanthamoeba polyphaga. Sixteen CL solutions were tested: 13 multiple purpose solutions (MPS), 2 hydrogen peroxidase solutions (HPS) and 1 povidone-iodine-based solution (PIS). The Spearman–Karber (SK) log reduction method and an XTT colorimetric assay were used to evaluate the effectiveness at the manufacturer’s minimum recommended disinfection time (MMRDT) and after eight hours. At the MMRDT, one MPS showed an SK mean log reduction (MLR) of >3.0 against A. castellanii trophozoites. Two additional MPS and both HPS reached this threshold after eight hours. The SK MLR values for A. polyphaga trophozoites were between 1 and 3 at all time points. Using the XTT colorimetric assay, only HPS 1 showed >99.9% reduction (equivalent to 3 log reduction) in metabolic activity of A. castellanii trophozoites after eight hours. For A. polyphaga, both HPS and PIS showed a metabolic reduction of >99.9% after eight hours. Cysts were resistant against all solutions. We conclude that following the manufacturer’s guidelines, few solutions provide sufficient effectiveness against Acanthamoeba trophozoites and none against cysts. The results underline the importance of adequate hygiene when handling CLs.
Functional Mapping of Genes Modulating Plant Shade Avoidance Using Leaf Traits
Authors: Zhang Han , Cao Yige , Wang Zijian , Ye Meixia , Wu Rongling 
Published: January 2023

Shade avoidance syndrome (SAS) refers to a set of plant responses that increases light capture in dense stands. This process is crucial for plants in natural and agricultural environments as they compete for resources and avoid suboptimal conditions. Although the molecular, biochemical, and physiological mechanisms underlying the SAS response have been extensively studied, the genetic basis of developmental variation in leaves in regard to leaf area, petiole length, and leaf length (i.e., their allometric relationships) remains unresolved. In this study, with the recombinant inbred line (RIL) population, the developmental traits of leaves of Arabidopsis were investigated under two growth density conditions (high- and low-density plantings). The observed changes were then reconstructed digitally, and their allometric relationships were modelled. Taking the genome-wide association analysis, the SNP genotype and the dynamic phenotype of the leaf from both densities were combined to explore the allometry QTLs. Under different densities, leaf change phenotype was analyzed from two core ecological scenarios: (i) the allometric change of the leaf area with leaf length, and (ii) the change of the leaf length with petiole length. QTLs modulating these two scenarios were characterized as ‘leaf shape QTLs’ and ‘leaf position QTLs’. With functional mapping, results showed a total of 30 and 24 significant SNPs for shapeQTLs and positionQTLs, respectively. By annotation, immune pathway genes, photosensory receptor genes, and phytohormone genes were identified to be involved in the SAS response. Interestingly, genes modulating the immune pathway and salt tolerance, i.e., systemic acquired resistance (SAR) regulatory proteins (MININ-1-related) and salt tolerance homologs (STH), were reported to mediate the SAS response. By dissecting and comparing QTL effects from low- and high-density conditions, our results elucidate the genetic control of leaf formation in the context of the SAS response. The mechanism with leaf development × density interaction can further aid the development of density-tolerant crop varieties for agricultural practices.
Constraining Co-Varying Coupling Constants from Globular Cluster Age
Authors: Gupta Rajendra P. 
Published: January 2023

Equations governing the evolution of a star involve multiple coupling constants. Thus, the time it spends as a main-sequence star can be expected to depend on whether or not such constants vary over the time scale of stellar evolution. When the star belongs to a globular cluster, the star’s age cannot exceed that of the globular cluster, and the latter cannot exceed the age of the Universe. This fact can be used to constrain or verify the variation of the coupling constants, i.e., the speed of light c, the gravitational constant G, the Planck constant h, and the Boltzmann constant k. We have estimated the age of the main-sequence star analytically from the time it takes to synthesize all its hydrogen into helium under fixed and varying coupling constants scenarios. When we permitted the interrelated variation of the four constants (G~c3~h3~k3/2) and differentiated between the cosmological energy and local energy conservation laws, we could show that the variation of the constants established in our earlier studies, i.e., G˙/G=3c˙/c=3h˙/h=1.5k˙/k=3.90±0.04×10−10 yr−1 at the current cosmic time is consistent with the present work. Nevertheless, the challenge remains to come up with an experiment, astrometric or terrestrial, that can unequivocally prove or falsify the predicted variation.
RESISTANCE OF SULFUR ASPHALT CONCRETE WITH RUBBER TO RUTS FORMATION
Authors: Ле Х.Т. , Ву Н.Т. , Герман В.В. 
Published: January 2023

При написании данной статьи был проведен ряд экспериментальных исследований по изучению стойкости аппретированной сероасфальтобетоновой смеси к колеобразованию. В результате проведенных исследований было выявлено влияние числа прохода колеса и типа модификатора серы на глубину колеи, а также на угол наклона кривой колееобразования. По итогам исследования был сделан вывод, что сероасфальтобетон лучше сопротивляется воздействию регулярной нагрузки от колес, чем другие виды материалов для производства асфальтобетона. Так, например, если в составе асфальтобетона есть 20% модификатора серы, то восприятие нагрузки увеличивается в 1,2 раза, если в смеси содержится 30% модификатора серы, то эта цифра увеличивается до 1,6, при 40% модификатора серы - до 2,3, а если смесь состоит из 50% модификатора серы, то увеличение происходит в 3,2 раза. Это говорит о том, что чем больше модификатора серы содержится в смеси, тем выше стойкость асфальта к колееобразованию.
When writing this article, a number of experimental studies were carried out to study the resistance of the finished sulfur-asphalt concrete mixture to rutting. As a result of the studies, the influence of the number of wheel passages and the type of sulfur modifier on the rut depth, as well as on the slope of the rutting curve, was revealed. According to the results of the study, it was concluded that sulfur asphalt concrete resists the effects of regular wheel loads better than other types of materials for the production of asphalt concrete. So, for example, if the asphalt concrete contains 20% sulfur modifier, then the load perception increases by 1.2 times, if the mixture contains 30% sulfur modifier, then this figure increases to 1.6, with 40% sulfur modifier - up to 2,3, and if the mixture consists of 50% sulfur modifier, then the increase is3.2 times. This suggests that the more sulfur modifier is contained in the mixture, the higher the rutting resistance of the asphalt.
Analisa Obat Dead Moving
Authors: Hariyani Hariyani , Rahmawati Anita 
Published: January 2023

Salah satu permasalahan instalasi farmasi dalam melakukan manajemen obat adalah terjadinya stok mati obat. Tujuan penelitian ini untuk menganalisa persentase obat dead moving apotek Rumah Sakit Widodo Ngawi. Desain penelitian ini adalah non ekperimental dengan pendekatan deskriptif. Populasi pada penelitian ini adalah seluruh jenis obat yang terdapat pada Apotek Rumah Sakit Widodo Ngawi tahun 2021 sebanyak 726 jenis obat. Teknik sampling dalam penelitian ini menggunakan total sampling. Hasil penelitian menunjukkan bahwa persentase obat dead moving Apotek Rumah Sakit Widodo Ngawi pada tahun 2021 adalah 1,10%. Hal ini menunjukkan bahwa pengelolaan obat masih belum efisien.
Analisis Kesesuaian Ekowisata Pantai Dan Bahari Di Pesisir Perairan Tanjung Kelapa Desa Poopoh Taman Nasional Bunaken
Authors: Sahri Muhammad Hasan , Pangemanan Paulus A. , Saroinsong Fabiola B. 
Published: January 2023

This research aims to analyze the suitability of coastal and marine ecotourism in the waters of Tanjung (Tj) Kelapa, Poopoh Village, Bunaken National Park (TN). The research was conducted from January to March 2022 in the coastal waters of Poopoh Village, Tombariri District, Minahasa Regency, North Sulawesi Province. This research uses the tourism suitability matrix analysis method. The data is taken from literature studies and direct field data measurements, displayed in the form of tables, graphs and images and processed by mathematical calculations for tourism suitability assessment. The research was conducted at 8 research stations, 2 research stations for beach recreation category, 2 research stations for snorkeling tourism category and 4 research stations for diving tourism category. The results showed that the suitability of tourism at the beach recreation research station was in the suitable category and very suitable, the snorkeling tour station was in the suitable category and at the diving tourism station 2 stations were in the suitable category and 2 stations were in the unsuitable category.
EDUKASI MASYARAKAT MENGENAI PELAPORAN EFEK SAMPING KOSMETIKA
Authors: Taurhesia Shelly , Farida Yunahara , Chaidir  , Umrah Siti , Djamil Ratna 
Published: January 2023

Kesadaran masyarakat untuk melaporkan efek samping kosmetik masih sangat rendah, padahal kosmetika digunakan setiap hari oleh berbagai lapisan masyarakat mulai dari bayi hingga lanjut usia, baik laki-laki dan perempuan. Ditambah inovasi kosmetik termasuk penggunaan bahan-bahan baru, relatif lebih cepat dibanding sediaan farmasi lainnya. BPOM sudah mengeluarkan Peraturan BPOM No. 26/2019 tentang Mekanisme Monitoring Efek samping Kosmetik (MESKOS), bahkan membentuk agent of change Pelaporan Efek Samping Kosmetik untuk meningkatkan efektivitas pelaporan.
Tujuan: Pengabdian kepada Masyarakat yang terintegrasi ini bertujuan untuk memberikan informasi kepada konsumen mengenai : 1) 3) mengenalkan tools serta cara melaporkan efek samping ke Badan Pengawas Obat dan Makanan (BPOM).
Metode: diawali dengan pemetaan calon peserta baik komunitas dan individu, penyesuaian materi agar mudah diikuti peserta yang beragam, dan memperhatikan susunan acara agar peserta mengikuti acara hingga selesai, dengan membuat Post Test menjadi bentuk permainan yang dilombakan. Kegiatan PKM dilaksanakan tanggal 18 Juni 2022, diikuti oleh lebih dari 400 peserta baik melalui zoom serta live streaming melalui youtube https://youtu.be/sVmhgSPvZ4I.
Kesimpulan: hasil evaluasi dari 222 peserta individu atas manfaat, efektivitas nara sumber dalam menyampaikan materi serta nilai keseluruhan mendapat rata-rata diatas 4,6 skala 5, sedangkan hasil pengukuran kepuasan mitra kerjasama dari 3 komunitas diperoleh nilai 5 skala 5. Acara ini diunggah dibeberapa media social seperti IG, FB dan mendapat respons positif
Collaborative Governance on Natural Disaster Management A Study on Forest and Land Fires in Central Kalimantan
Authors: Ruswandi Dody 
Published: January 2023

<em>Forest and land fires repeatedly occur each year in Indonesia, with a variety of sources that bring them about. There are 6 (six) provinces in Indonesia that have always experienced forest and land fires, because these six provinces have the largest peatland in comparison to other provinces. The Central Kalimantan Province experienced the largest forest and land fires in 2015 and 2019. Forest and land fires management involves multi-stakeholders both from the national and regional level. The research was conducted in Pulang Pisau Regency, to examine the collaborative collaboration process between stakeholders that have not functioned optimally. This is why forest and land fires keep on reoccurring annually. The authors conducted an analysis of several existing collaborative governance theories and based on the results of the subsequent analysis the authors recommended a collaboration governance model that is suitable to be applied to fire management on land specifically in Central Kalimantan and Indonesia in general. Here, the author reconstructs the collaborative governance theory of Ansell &amp; Gash (2007) by adding a wisdom variable to become a new variable in implementing collaborative governance in forest and land fire disaste management in Central Kalimantan.</em>