bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expanding the reproductive organ phenotype of CHD7‐spectrum disorder
Authors: Nomakuchi Tomoki T. , Danowitz Melinda , Stewart Blythe , Leonard Jacqueline , Izumi Kosuke , Krantz Ian , Kolon Thomas F. , Langdon David , Skraban Cara , Van Batavia Jason , Zackai Elaine , Jiao Kai , Linn Rebecca , Alexander Caitlin , Zaontz Mark , Vogiatzi Maria G. , Pyle Louise C. 
Published: February 2023

AbstractCHD7 disorder is a multiple congenital anomaly syndrome with a highly variable phenotypic spectrum, and includes CHARGE syndrome. Internal and external genital phenotypes frequently seen in CHD7 disorder include cryptorchidism and micropenis in males, and vaginal hypoplasia in females, both thought to be secondary to hypogonadotropic hypogonadism. Here, we report 14 deeply phenotyped individuals with known CHD7 variants (9 pathogenic/likely pathogenic and 5 VOUS) and a range of reproductive and endocrine phenotypes. Reproductive organ anomalies were observed in 8 of 14 individuals and were more commonly noted in males (7/7), most of whom presented with micropenis and/or cryptorchidism. Kallmann syndrome was commonly observed among adolescents and adults with CHD7 variants. Remarkably, one 46,XY individual presented with ambiguous genitalia, cryptorchidism with Müllerian structures including uterus, vagina and fallopian tubes, and one 46,XX female patient presented with absent vagina, uterus and ovaries. These cases expand the genital and reproductive phenotype of CHD7 disorder to include two individuals with genital/gonadal atypia (ambiguous genitalia), and one with Müllerian aplasia.
Carbon‐Carbon Bond Formation from Carbon Monoxide and Hydride: The Role of Metal Formyl Intermediates**
Authors: Parr Joseph M. , Crimmin Mark R. 
Published: February 2023

AbstractCurrent examples of carbon chain production from metal formyl intermediates with homogeneous metal complexes are described in this Minireview. Mechanistic aspects of these reactions as well as the challenges and opportunities in using this understanding to develop new reactions of CO and H2 are also discussed.
Microstructuring process in oleogels formulated with vegetable oils and monoglycerides: A comparison of non‐isothermal nucleation kinetics by spectrophotometric and DSC analysis
Authors: Contreras‐Ramírez Javier Isaac , Acosta‐Gurrola Esperanza , Rosas‐Flores Walfred , Gallegos‐Infante José Alberto , Toro‐Vázquez Jorge Fernando 
Published: February 2023

AbstractThe initial oleogelation process (microstructuring) as well as the formulation are determinant to obtain the desired characteristics in oleogels with potential application in the industry. The microstructuring process in oleogels has been extensively studied by means of techniques highly sensitive to thermal variations, such as differential scanning calorimetry (DSC). However, there are other readily available techniques and equipment that can be employed to perform similar evaluations. Non‐isothermal nucleation kinetics by spectrophotometric methods can be used as alternatives to basic crystallization studies in oleogels. Therefore, in this research a comparison of both techniques is presented, highlighting their similarities, advantages and limitations, in the study of the microstructure of oleogels. Oleogels were obtained with a minimum concentration of gelator and another saturated one, using vegetable oils of different degrees of saturation. The crystallization profiles of the oleogels were obtained by DSC, a non‐isothermal nucleation kinetics was performed from the molten system and the final microstructure was evaluated by optical microscopy. The Fisher‐Turnbull and Avrami model was used to evaluate the behavior during microstructuring. A gap was observed during the crystallization process by DSC which can be evaluated by spectrophotometry. Differences in the microstructuring process were found in both methods due to the temperature ramp used and formulation variables. The results obtained by spectrophotometry indicate that it can be a good alternative, easily accessible in oleogel crystallization studies, when high sensitivity or very specific thermal parameters are not required.
Simulation and optimized design of cure cycle for three‐dimensional polymer‐matrix composite component
Authors: Yang Wenkai , Zhao Che , Jia Yunlong , Lu Shihong 
Published: February 2023

AbstractThe cure cycle plays a crucial role in manufacturing polymer‐matrix composite component. Unreasonable design of cure cycle can easily lead to thermal gradients during curing process, which will generate residual stress and seriously affect the quality of component. Meanwhile, the cure cycle of the three‐dimensional composite component is difficult to optimized, due to the complicated distribution of temperature and curing degree. In this paper, numerical simulation is applied to research the curing process of the polymer‐matrix composite. The change rules of temperature and curing degree are explored. An optimum design method for the cure cycle of three‐dimensional composite component is proposed. Meanwhile, numerical simulation is applied in this method to design the cure cycle by referring to the change rules of temperature and curing degree of the component, in which the internal and external thermal gradients can be balanced during the curing process. The results show that the optimized cure cycle can significantly reduce the thermal gradients for three‐dimensional composite component, which provide guidance for cure cycle design in the manufacturing of polymer‐matrix composite.
Enhanced mechanical, tribological, and acoustical behavior of polyphenylene sulfide composites reinforced with zero‐dimensional alumina
Authors: Natarajan Elango , Ramesh S. , Markandan Kalaimani , Saravanakumar N. , Dilip A. Anto , Batcha Abdul Rahim Sadiq 
Published: February 2023

AbstractPolyphenylene sulfide (PPS) is a semicrystalline thermoplastic which can pass UL94V‐0 standard without adding flame retardant. In the present study, effect of alumina (Al2O3) addition on the mechanical, tribological, and acoustical behavior of PPS polymer were investigated. Experimental findings indicate that elastic modulus, tensile strength, and flexural strength were enhanced by 21.4%, 7.7%, and 6.9%, respectively. The highest tensile strength was obtained when PPS was reinforced with 5 wt% Al2O3 while highest bending strength and toughness were obtained with 20 wt% Al2O3. Reinforced composites encountered <5% and <14% mass loss when heated to 420 and 500°C, respectively. Lower filler concentrations (<5 wt%) had insignificant effect on the glass transition temperature (Tg) of the composite. Experimental results agreed well to Hashin–Shtrikman and Suquet predictions at lower filler concentrations since filler particles could be easily dispersed in the interstitial sites of PPS matrix without significant agglomeration. Besides, significant acoustic emission (AE) characteristic such as higher absolute energy rate was witnessed in Al2O3 reinforced PPS composites. The insights derived from this study are expected to open a new avenue of producing composites with high stiffness, strength, and significant AE events by using zero‐dimensional fillers.
Wildfire damage and contamination to private drinking water wells
Authors: Jankowski Caroline , Isaacson Kristofer , Larsen Madeline , Ley Christian , Cook Myles , Whelton Andrew J. 
Published: February 2023

AbstractFollowing the 2021 Marshall Fire in Colorado, this study was conducted to better understand private well and plumbing damage and to develop public health guidance. More than 20 post‐fire drinking water well guidance documents with varied recommendations were found. Approximately 227 wells were located in the fire footprint. Seventeen properties were visited, and a subset of wells were sampled for organic and inorganic contaminants. Property debris was also collected. Benzene, toluene, and 19 semi‐volatile organic compounds (SVOCs) were detected in water extracts of property debris. No wells contained volatile organic compound contamination. Two shallow wells (12 and 15 ft) had debris contamination; one well contained notable SVOC contamination. One multi‐home unregulated well system was extensively damaged, lost pressure, and had not been repressurized 11 months after the fire due to financial and technical challenges. Study results highlight the need for follow‐up work to understand well system damage and household response.
microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease
Authors: Yao Yufang , Zhao Zhiyue , Zhang Fubo , Miao Na , Wang Nan , Xu Xin , Yang Chaoping 
Published: February 2023

AbstractBackgroundParkinson's disease (PD) is one of the most common systemic neurodegenerative diseases and is related to the loss of dopaminergic neurons in the substantia nigra. Several studies verified that microRNA (miRNAs) targeting the Bim/Bax/caspase‐3 signaling axis is involved in the apoptosis of dopaminergic neurons in substantia nigra. In this study, we aimed to explore the role of miR‐221 in PD.MethodsTo examine the function of miR‐221 in vivo, we used a well‐established 6‐OHDA‐induced PD mouse model. Then we conducted adenovirus‐mediated miR‐221 overexpression in the PD mice.ResultsOur results showed that miR‐221 overexpression improved motor behavior of the PD mice. We demonstrated that overexpression of miR‐221 reduced the loss of dopaminergic neurons in the substantia nigra striatum by promoting their antioxidative and antiapoptosis capacities. Mechanistically, miR‐221 targets Bim, thus inhibiting Bim and Bax caspase‐3 mediated apoptosis signaling pathways.ConclusionOur findings suggest miR‐221 participates in the pathological process of PD and might be a potential drug target and provide new insight into PD treatment.
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts
Authors: Barbieri Gilcelia J. L. S. , Simão Estéfani P. , Avelino Karen Y. P. S. , Oliveira Maria D. L. , Andrade Cesar A. S. 
Published: February 2023

AbstractThis article developed a novel electrochemical immunosensor for the specific detection of aflatoxin B1 (AFB1). Amino‐functionalized iron oxide nanoparticles (Fe3O4‐NH2) were synthesized. Fe3O4‐NH2 were chemically bound on self‐assembly monolayers (SAMs) of mercaptobenzoic acid (MBA). Finally, polyclonal antibodies (pAb) were immobilized on Fe3O4‐NH2‐MBA. The sensor system was evaluated through atomic force microscopy (AFM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). A reduction in the anodic and cathodic peak currents was observed after the assembly of the sensor platform. The charge transfer resistance (Rct) was increased due to the electrically insulating bioconjugates. Then, the specific interaction between the sensor platform and AFB1 blocks the electron transfer of the [Fe(CN)6]3−/4− redox pair. The nanoimmunosensor showed a linear response range estimated from 0.5 to 30 μg/mL with a limit of detection (LOD) of 9.47 μg/mL and a limit of quantification (LOQ) of 28.72 μg/mL for AFB1 identification in a purified sample. In addition, a LOD of 3.79 μg/mL, a LOQ of 11.48 μg/mL, and a regression coefficient of 0.9891 were estimated for biodetection tests on peanut samples. The proposed immunosensor represents a simple alternative, successfully applied in detecting AFB1 in peanuts, and therefore, represents a valuable tool for ensuring food safety.
Monocyte chemoattractant protein 1 as a potential biomarker for immune checkpoint inhibitor‐associated neurotoxicity
Authors: Möhn Nora , Mahjoub Susann , Duzzi Laura , Narten Emily , Grote‐Levi Lea , Körner Gudrun , Seeliger Tabea , Beutel Gernot , Bollmann Benjamin‐Alexander , Wirth Thomas , Huss André , Tumani Hayrettin , Grimmelmann Imke , Gutzmer Ralf , Ivanyi Philipp , Skripuletz Thomas ,   ICOG‐CCCH (Immune Cooperative Oncology Group; Comprehensive Cancer Center Hannover)
Published: February 2023

AbstractBackgroundOncological patients can benefit substantially from treatment with immune checkpoint inhibitors (ICI). However, there is a growing awareness of immune‐related adverse events (irAE). Especially ICI‐mediated neurological adverse events (nAE(+)), are tough to diagnose and biomarkers to identify patients at risk are missing.MethodsA prospective register with prespecified examinations was established for ICI treated patients in December 2019. At the time of data cut‐off, 110 patients were enrolled and completed the clinical protocol. Herein, cytokines and serum neurofilament light chain (sNFL) from 21 patients were analyzed.ResultsnAE of any grade were observed in 31% of the patients (n = 34/110). In nAE(+) patients a significant increase in sNFL concentrations over time was observed. Patients with higher‐grade nAE had significantly elevated serum‐concentrations of monocyte chemoattractant protein 1 (MCP‐1) and brain‐derived neurotrophic factor (BDNF) at baseline compared to individuals without any nAE (p < 0.01 and p < 0.05).ConclusionHere, we identified nAE to occur more frequently than previously reported. Increase of sNFL during nAE confirms the clinical diagnosis of neurotoxicity and might be a suitable marker for neuronal damage associated with ICI therapy. Furthermore, MCP‐1 and BDNF are potentially the first clinical‐class nAE predictors for patients under ICI therapy.
Facile Synthesis of Palladium‐Silver Dilute Alloy Catalyst for Acetylene Hydrogenation
Authors: Chai Shanshan , Gao Denglei , Xia Jing , Yang Yanpeng , Wang Xi 
Published: February 2023

AbstractSelective hydrogenation of acetylene (C2H2) to ethylene (C2H4) has been recognized as an important strategic reaction for the removal of trace acetylene from ethylene. Palladium‐based alloys are one of the most commonly used catalysts for this reaction but bear a high price and unsatisfactory catalytic performance. Here, we develop a feasible strategy toward the synthesis of a palladium‐silver dilute alloy catalyst (PdxAg/Al2O3), which contains the Pd1Ag single‐atom‐alloy (SAA) and Pd2Ag dimer‐alloy (DA) species. This catalyst exhibits a high ethylene (C2=) yield of 90.1 % even after 100 hours at 60 °C, which is 11.9 times higher than that of the PdAg/Al2O3 alloy catalyst. Based on in‐situ spectroscopic investigations and theoretical calculations, both Pd1Ag SAA and Pd2Ag DA species in PdxAg/Al2O3 are easier to desorb C2H4 compared with PdAg/Al2O3, and they experience the possible hydrogenation paths with the low energy barriers to yield C2=. Furthermore, PdxAg/Al2O3 is beneficial to restraining coking due to the endothermic C−H bond cleavage. Thus, these combined merits contribute to the superior catalytic performance for PdxAg/Al2O3.
Development of Enterovirus Antiviral Agents That Target the Viral 2C Protein
Authors: Kejriwal Rishabh , Evans Tristan , Calabrese Joshua , Swistak Lea , Alexandrescu Lauren , Cohen Michelle , Rahman Nahian , Henriksen Niel , Charan Dash Radha , Hadden M. Kyle , Stonehouse Nicola J. , Rowlands David J. , Kingston Natalie J. , Hartnoll Madeline , Dobson Samuel J. , White Simon J. 
Published: February 2023

AbstractThe Enterovirus (EV) genus includes several important human and animal pathogens. EV‐A71, EV‐D68, poliovirus (PV), and coxsackievirus (CV) outbreaks have affected millions worldwide, causing a range of upper respiratory, skin, and neuromuscular diseases, including acute flaccid myelitis, and hand‐foot‐and‐mouth disease. There are no FDA‐approved antiviral therapeutics for these enteroviruses. This study describes novel antiviral compounds targeting the conserved non‐structural viral protein 2C with low micromolar to nanomolar IC50 values. The selection of resistant mutants resulted in amino acid substitutions in the viral capsid protein, implying these compounds may play a role in inhibiting the interaction of 2C and the capsid protein. The assembly and encapsidation stages of the viral life cycle still need to be fully understood, and the inhibitors reported here could be useful probes in understanding these processes.
Bimetallic Metal‐Organic Frameworks (BMOF) and BMOF‐ Incorporated Membranes for Energy and Environmental Applications
Authors: Chithra K. R. , Rao Shashank M. , Varsha M. V. , Nageswaran Gomathi 
Published: February 2023

AbstractBimetallic metal organic frameworks (BMOFs) are a class of crystalline solids and their structure comprises two metal ions in the lattice. BMOFs show a synergistic effect of two metal centres and enhanced properties compared to MOFs. By controlling the composition and relative distribution of two metal ions in the lattice the structure, morphology, and topology of BMOFs could be regulated resulting in an improvement in the tunability of pore structure, activity, and selectivity. Thus, developing BMOFs and BMOF incorporated membranes for applications such as adsorption, separation, catalysis, and sensing is a promising strategy to mitigate environmental pollution and address the looming energy crisis. Herein we present an overview of recent advancements in the area of BMOFs and a comprehensive review of BMOF incorporated membranes reported to date. The scope, challenges as well as future perspectives for BMOFs and BMOF incorporated membranes are presented.
High Entropy Oxide (CrMnFeNiMg)3O4 with Large Compositional Space Shows Long‐Term Stability as Cathode in Lithium‐Sulfur Batteries
Authors: Chung Sheng‐Heng , Wu Yi‐Hsuan , Tseng Yu‐Hsun , Nguyen Thi Xuyen , Ting Jyh‐Ming 
Published: February 2023

AbstractThe repeated formation and irreversible diffusion of liquid‐state lithium polysulfides (LiPSs) are the primary challenges in the development of high‐energy‐density lithium‐sulfur battery (LSB). An effective strategy to alleviate the resulting polysulfide loss is critical for the stability of LSBs. In this regard, high entropy oxides (HEOs) appear as a promising additive for the adsorption and conversion of LiPSs owing to the diverse active sites, offering unparalleled synergistic effects. Herein, we have developed a (CrMnFeNiMg)3O4 HEO as a functional polysulfide trapper in LSB cathode. The adsorption of LiPSs by the metal species (i. e., Cr, Mn, Fe, Ni, and Mg) in the HEO takes place through two different paths and leads to enhanced electrochemical stability. We demonstrate that the optimal sulfur cathode with the (CrMnFeNiMg)3O4 HEO attains a high peak and reversible discharge capacities of 857 mAh g−1 and 552 mAh g−1, respectively, at a cycling rate of C/10, a long cycle life of 300 cycles, and a high rate performance at the cycling rates from C/10 to C/2.
pMOS‐only pW‐power voltage reference with sub‐10 ppm/°C trimmed temperature coefficient and sub‐100 ppm/V line sensitivity
Authors: Azimi Mohammad , Habibi Mehdi , Crovetti Paolo 
Published: February 2023

SummaryIn this paper, a new ultra‐low‐power voltage reference based on a two‐stage, all‐pMOS topology operating in the subthreshold region is proposed to uniquely meet the pW‐power range power consumption requirements of emerging Internet‐of‐Things applications without significantly compromising the temperature coefficient (TC) and the line sensitivity (LS) performance. The proposed circuit consists of the LS regulator, TC corrector, and TC trimming sections. Based on post‐layout Monte Carlo simulations in 180‐nm CMOS, the proposed circuit operates with 0.8‐ to 2.4‐V supply potential and generates a reference voltage of 206 mV with a process spread of 7.8%, achieving an average calibrated TC of 4.4 ppm/°C in the temperature range of −20°C to 80°C, and an average LS of 51.5 ppm/V with a power consumption of 25.9 pW at 25°C (469.1 pW at 80°C).
ALKBH5‐mediated m6A demethylation of TIRAP mRNA promotes radiation‐induced liver fibrosis and decreases radiosensitivity of hepatocellular carcinoma
Authors: Chen Yuhan , Zhou Peitao , Deng Yixun , Cai Xinni , Sun Mingrui , Sun Yining , Wu Dehua 
Published: February 2023

AbstractBackgroundRadiation‐induced hepatic stellate cell (HSC) activation promotes radiation‐induced liver fibrosis (RILF), a complication for hepatocellular carcinoma (HCC) radiotherapy. The demethylase alpha‐ketoglutarate‐dependent dioxygenase alkB homolog 5 (ALKBH5) decreases N6‐methyladenylate methylation (m6A) modification of RNA, while its role in regulating RILF pathogenesis and HCC radiosensitivity remains unknown.MethodsMethylated RNA immunoprecipitation sequencing (MeRIP‐seq) and RNA‐sequencing (RNA‐seq) were used to screen target genes regulated by ALKBH5. HSC with altered ALKBH5 expression was used to assess irradiation‐induced HSC activation and the effect of HSC on recruitment and polarisation of monocytes. Key cytokines in medium from irradiated HSC‐educated monocytes were identified by cytokine array detection. The effects of blocking ALKBH5 and key cytokines on RILF and HCC radiosensitivity were also evaluated.ResultsRadiation‐induced ALKBH5 expression in HSC mediated m6A demethylation of toll‐interleukin 1 receptor domain containing adaptor protein (TIRAP) mRNA and activated its downstream NF‐κB and JNK/Smad2 pathways to promote HSC activation. Additionally, ALKBH5 regulated CCL5 secretion by irradiated HSC to promote monocyte recruitment and M2 macrophage polarisation. Notably, polarised monocytes secreted CCL20 to up‐regulate ALKBH5 expression in HSC, and reduce HCC radiosensitivity by activating ALKBH5/TIRAP axis in HCC cells. ALKBH5 knockdown‐combined CCR6 (CCL20 receptor) inhibitor significantly alleviated RILF and improved HCC radiosensitivity in mice. HCC patients with high ALKBH5 and TIRAP expression were prone to radiation‐induced liver injury and poor tumour response to radiotherapy.ConclusionsCollectively, irradiation up‐regulates ALKBH5 in HSC to mediate monocyte recruitment and M2 polarisation and form positive feedback to promote RILF and reduce HCC radiosensitivity. The dual roles of ALKBH5 as a microenvironmental regulator and radiosensitisation target provide new ideas for RILF prevention and radiosensitisation of HCC.