bluuebunny/crossref_metadata_2025_split huggingface dataset:
Marjolin’s ulcer in an ischial pressure sore presented with necrotizing soft tissue infection: A case report
Authors: Huang Ching-Yen , Hsieh Zhoa-Yu , Chang Ke-Chung , Chang Dun-Hao 
Published: March 2023

Rationale:
Marjolin’s ulcer (MU) is a rare skin malignancy derived from a chronic wound. Pressure ulcer related MU possesses poor prognosis and high metastatic rate, and it is difficult to be differentiated, especially when superimposed infection.

Patient concerns:
Here we report a case with pressure ulcer related MU which presented as necrotizing soft tissue infection (NSTI) to demonstrate the manifestation, treatment, and prognosis of this rare disease.

Diagnoses:
A 45-year-old male patient had spinal cord injury at age 2 years. He presented ischial pressure sore complicated with NSTI initially. After serial debridements and antibiotic treatment, the infection subsided. For the persistent verruca-like skin lesion, he underwent wide excision which revealed well-differentiated squamous cell carcinoma. Further image studies showed localized residual tumor without distant metastasis.

Interventions:
He then underwent hip disarticulation and anterior thigh fillet flap reconstruction. Local recurrence developed 3 months later, and re-wide excision and inguinal lymph node dissection were performed. No lymph node metastasis was noted and adjuvant radiotherapy was given.

Outcomes:
He was followed for 34 months and no recurrence or metastasis was found. The patient can move with a wheelchair or a hip prosthesis, and is partially dependent for daily activities.

Lessons:
MU can masquerade as NSTI and one should be alert to its malignant potential. Due to its aggressive nature, limb sacrifice can be considered in circumstances of profound involvement. As for the reconstruction method, pedicled fillet flap provided good wound coverage.
Somatic chromosome pairing has a determinant impact on 3D chromatin organization
Authors: Puerto Marta , Shukla Mamta , Bujosa Paula , Perez-Roldan Juan , Tamirisa Srividya , Solé Carme , Nadal Eulàlia de , Posas Francesc , Azorin Fernando , Rowley M. Jordan 
Published: March 2023

ABSTRACTIn the nucleus, chromatin is intricately structured into multiple layers of 3D organization important for genome activity. How distinct layers influence each other is not well understood. In particular, the contribution of chromosome pairing to 3D chromatin organization has been largely neglected. Here, we address this question inDrosophila,an organism that shows robust chromosome pairing in interphasic somatic cells. The extent of chromosome pairing depends on the balance between pairing and anti-pairing factors, with the anti-pairing activity of the CAP-H2 condensin II subunit being the best documented. Here, we identify the zinc-finger protein Z4 as a strong anti-pairer that interacts with and mediates the chromatin binding of CAP-H2. We also report that hyperosmotic cellular stress induces fast and reversible chromosome unpairing that depends on Z4/CAP-H2. And, most important, by combining Z4 depletion and osmostress, we show that chromosome pairing reinforces intrachromosomal 3D interactions. On the one hand, pairing facilitates RNAPII occupancy that correlates with enhanced intragenic gene-loop interactions. In addition, acting at a distance, pairing reinforces chromatin-loop interactions mediated by Polycomb (Pc). In contrast, chromosome pairing does not affect which genomic intervals segregate to active (A) and inactive (B) compartments, with only minimal effects on the strength of A-A compartmental interactions. Altogether, our results unveil the intimate interplay between inter-chromosomal and intra-chromosomal 3D interactions, unraveling the interwoven relationship between different layers of chromatin organization and the essential contribution of chromosome pairing.
AutophagyNet: High-resolution data source for the analysis of autophagy and its regulation
Authors: Csabai Luca , Bohár Balázs , Türei Dénes , Prabhu Sowmya , Földvári-Nagy László , Madgwick Matthew , Fazekas Dávid , Módos Dezső , Ölbei Márton , Halka Themis , Poletti Martina , Kornilova Polina , Kadlecsik Tamás , Demeter Amanda , Szalay-Bekő Máté , Kapuy Orsolya , Lenti Katalin , Vellai Tibor , Gul Lejla , Korcsmáros Tamás 
Published: March 2023

AbstractAutophagy is a highly-conserved catabolic process eliminating dysfunctional cellular components and invading pathogens. Autophagy malfunction contributes to disorders such as cancer, neurodegenerative and inflammatory diseases. Understanding autophagy regulation in health and disease has been the focus of the last decades. We previously provided an integrated database for autophagy research, the Autophagy Regulatory Network (ARN). For the last seven years, this resource has been used by thousands of users. Here, we present a new and upgraded resource, AutophagyNet. It builds on the previous database but contains major improvements to address user feedback and novel needs due to the advancement in omics data availability. AutophagyNet contains updated interaction curation and integration of over 280,000 experimentally verified interactions between core autophagy proteins and their protein, transcriptional and post-transcriptional regulators as well as their potential upstream pathway connections. AutophagyNet provides annotations for each core protein about their role: 1) in different types of autophagy (mitophagy, xenophagy, etc.); 2) in distinct stages of autophagy (initiation, elongation, termination, etc); 3) with subcellular and tissue-specific localization. These annotations can be used to filter the dataset, providing customizable download options tailored to the user’s needs. The resource is available in various file formats (e.g., CSV, BioPAX and PSI-MI), and data can be analyzed and visualized directly in Cytoscape. The multi-layered regulation of autophagy can be analyzed by combining AutophagyNet with tissue- or cell type-specific using (multi-)omics datasets (e.g. transcriptomic or proteomic data). The resource is publicly accessible athttp://autophagynet.org.
Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia
Authors: Castaneda Diana Cadena , Jangra Sonia , Yurieva Marina , Martinek Jan , Callender Megan , Coxe Matthew , Choi Angela , Diego Juan García-Bernalt , Lin Jianan , Wu Te-Chia , Marches Florentina , Chaussabel Damien , Yu Peter , Salner Andrew , Aucello Gabrielle , Koff Jonathan , Hudson Briana , Church Sarah E. , Gorman Kara , Anguiano Esperanza , García-Sastre Adolfo , Williams Adam , Schotsaert Michael , Palucka Karolina 
Published: March 2023

AbstractThe COVID-19 pandemic continues to be a health crisis with major unmet medical needs. The early responses from airway epithelial cells, the first target of the virus regulating the progression towards severe disease, are not fully understood. Primary human air-liquid interface cultures representing the broncho-alveolar epithelia were used to study the kinetics and dynamics of SARS-CoV-2 variants infection. The infection measured by nucleoprotein expression, was a late event appearing between day 4-6 post infection for Wuhan-like virus. Other variants demonstrated increasingly accelerated timelines of infection. All variants triggered similar transcriptional signatures, an “early” inflammatory/immune signature preceding a “late” type I/III IFN, but differences in the quality and kinetics were found, consistent with the timing of nucleoprotein expression. Response to virus was spatially organized: CSF3 expression in basal cells and CCL20 in apical cells. Thus, SARS-CoV-2 virus triggers specific responses modulated over time to engage different arms of immune response.
“Understanding the journey from A to Z”: centering peer support perspectives to unveil the mechanisms and power of peer support
Authors: Rao Sandy , Dimitropoulos Gina 
Published: March 2023

Purpose
This study aims to gain insights from peer support workers (PSWs) on the unseen mechanisms of peer support work that may be applied to implementing a child and youth mental health peer support program in a children’s hospital emergency department (ED).

Design/methodology/approach
This study used a critical realist perspective with a qualitative research design. Eight participants, all PSWs in health, community and social services working with adults, adolescents and children, were recruited from Alberta, Canada. Data were collected through in-person semi-structured interviews and analyzed using Braun and Clarke’s reflexive thematic analysis.

Findings
The results generated interrelated patterns of meaning among PSWs that were grouped into three main themes: inside out and outside in, no one leaves empty-handed and sculpting health system softness.

Research limitations/implications
All participants were trained by the same organization and may have similar understandings and approaches to the peer support role. Participants were predominantly of a similar demographic; thus, participants with minoritized identities may have challenges not represented.

Practical implications
Using nontraditional staffing models in an acute center may facilitate a shift to meet the chronic and complex issues of the 21st century.

Social implications
Youth peer support roles may reduce stigma by challenging stereotypes and myths, reduce social isolation and improve meaning, connection and belonging for individuals with mental illness who are often dislocated and disconnected from society.

Originality/value
This study exemplified the positive impacts peer support could make by improving child, youth and family experiences; reducing stigma; providing hope and help; and embedding the recovery model directly into the ED.
Driving the sustainability agenda: a wakeup call for hospitality education
Authors: Munjal Sandeep , Sharma Sanjay 
Published: March 2023

PurposeThis concluding article identifies the varying perspectives and research findings that emanate from the collection of articles in this theme issue. It also revisits the theme issue question and provides some directions for further research in the form of a call for action based on the key findings of this issue. It is apparent that there is an urgent need for hospitality education in the higher education sector to give space and focus to sustainability practices in their curriculum and ensure that the content is embedded and delivered in a contemporary way. Further, the faculty should be up-to-date and understand their role as drivers of the sustainability footprint for tourism and hospitality. The authors argue that hospitality education not only needs to catch up but also make a determined effort to lead applied research that can inform the industry in terms of what and how more can be done to mitigate the negative impacts of tourism activity.Design/methodology/approachThe paper draws on a content analysis of the article collection in this theme issue: How is hospitality education embedding sustainable practices in their curriculum and programme delivery? An Asian perspective is considered here in order to summarize, conclude and suggest some next steps.FindingsThere is an urgent need to support and advance the cause of sustainability in tourism and hospitality, especially in the Asian context. The paper identifies key steps that need to be taken by the hospitality higher education sector in order to contribute to the larger goal of sustainable practices that can be easily understood and adopted by those managing hospitality businesses.Originality/valueThe paper documents the theme issue findings and provides a collaborative research agenda based on the findings of the theme issue writing team.
UNRAVELLED HOMES: Forced Evictions and Home Remaking in Jakarta
Authors: Siagian Clara , Utomo Ariane , Kamil Muhammad Insan , Cook Brian 
Published: March 2023

AbstractThis article explores the contours of modernization in the unmaking and remaking of homes among evicted and resettled families in highrise housing. We examine the trajectories of forced eviction by drawing upon interviews with 17 individuals from nine evicted families who have transitioned from living in informal settlements to highrise social housing (rusunawa) in Jakarta. Drawing on two strands of literature—‘developmental idealism and the family’ from population studies and the critical geographies of ‘homemaking’—we argue that the demolition of houses is but an initial event in a long, quiet and subtle, yet profoundly defining, process of ‘upgrading’ families as part of ‘improving’ society, according to developmental logic. The disciplining of the urban poor does not end with the demolition of their houses, but rather continues as part of the fulfilment of shelter. This article attends to the slow unravelling of home hidden and embedded in post‐eviction everyday lives, which are often overlooked because of the overt and violent brutality of forced eviction. While eviction can be seen as the violent visual expression of developmentalism, we argue that the relocation in rusunawa is where this ideal permeates into daily domestic life, making mundane activities a battleground for different ideals of ‘home’.
How the COVID‐19 crisis affected the well‐being of nurses working in paediatric critical care: A qualitative study
Authors: Pountney Jackson , Butcher Isabelle , Donnelly Peter , Morrison Rachael , Shaw Rachel L. 
Published: March 2023

AbstractObjectivesEvidence shows paediatric critical care (PCC) nurses display high rates of burnout, moral distress, symptoms associated with post‐traumatic stress disorder (PTSD) and poor well‐being. The COVID‐19 pandemic magnified these pressures producing extremely challenging working conditions. The objective was to understand PCC nurses' lived experience of working during COVID‐19 to determine the impact it had on their well‐being.DesignA qualitative design was used with individual, semi‐structured online interviews analysed using thematic analysis.ResultsTen nurses from six PCC units in England participated. Five themes were generated: (i) Challenges of working in Personal Protective Equipment (PPE), (ii) Adapting to redeployment to adult intensive care, (iii) Changes to staff working relationships, (iv) Being unable to attain work‐life balance and (v) Unprocessed traumatic experiences of working in COVID‐19. It was clear COVID‐19 presented novel challenges to PCC nurses' well‐being. With those came enforced changes in practice; some were temporary, for example use of PPE and redeployment, but others provided insight into the prerequisites for good staff well‐being, for example strong professional relationships, work–life balance and managing one's psychological health.ConclusionsFindings show authentic connections between peers, verbal and non‐verbal communication and a sense of belonging were crucial to nurses' well‐being. A dent in PCC nurses' perceived competence significantly affected their well‐being. Finally, staff need a psychologically safe space to process distress and trauma experienced during COVID‐19. Future research needs to test evidence‐based, theoretically‐informed well‐being interventions to improve and maintain PCC nurses' well‐being.
Changes in biomarkers of endothelial function, oxidative stress, inflammation and lipids after smoking cessation: A cohort study
Authors: Colsoul Marie‐Lise , Goderniaux Nicolas , Onorati Sabrina , Dupuis Stéphanie , Jamart Jacques , Vanpee Dominique , Berlin Ivan , Galanti Laurence 
Published: March 2023

AbstractBackgroundTobacco use is known to be involved in the development of cardiovascular diseases, which leads to premature mortality. Endothelial dysfunction, the first step in this process, was shown induced by smoking. It is reported that quitting smoking could reduce the risk of diseases, but the implied mechanisms are still unclear. This study aimed to evaluate the biological markers of endothelial function in smokers when actively smoking and after cessation.MethodsQuantification of several biomarkers reflecting inflammation, endothelium activation, oxidative stress, and lipids was performed in 65 smokers when actively smoking and after cessation (median abstinence duration of 70 days).ResultsA possible decrease of inflammation was observed through the concentration reduction of a proinflammatory cytokine (interleukine‐6) on quitting. A decrease of endothelium activation was visible by the reduced level of the soluble intercellular adhesion molecule. Two antioxidants, uric acid and vitamin C, were found at higher concentration than before the cessation, potentially reflecting the decrease of oxidative stress on quitting. Lipid profile was improved post‐quit since HDL level was increased and LDL level was decreased. All these effects were visible at short term with abstinence duration less than 70 days. No sex‐specific difference was observed and no additional changes were observed for longer abstinence duration.ConclusionThese observations suggest that some adverse effects of smoking on endothelial function could be reversible on quitting smoking. It could encourage smokers to enter a cessation program to reduce the risk for cardiovascular diseases development.
Acupuncture for treatment of knee osteoarthritis: A clinical practice guideline
Authors: Luo Xiaochao , Liu Jiali , Li Qianrui , Zhao Jiping , Hao Qiukui , Zhao Ling , Chen Yemeng , Yin Pengbin , Li Ling , Liang Fanrong , Sun Xin 
Published: March 2023

AbstractClinical questionIs acupuncture effective in treating knee osteoarthritis (KOA)?Current practiceAlthough increasingly used in the clinical setting, acupuncture is not mentioned or weakly recommended in guidelines for the treatment of KOA.RecommendationsWe suggest acupuncture rather than no treatment in adult KOA (weak recommendation, moderate certainty evidence), and acupuncture combined with nonsteroidal anti‐inflammatory drugs (NSAIDs) rather than acupuncture alone when KOA symptoms are severe (weak recommendation, moderate certainty evidence), with duration of acupuncture for 4–8 weeks depending on KOA severity and treatment response (weak recommendation, moderate certainty evidence), and discussing with patients in shared decision‐making.How this guideline was createdThis rapid recommendation was developed following the Making GRADE the Irresistible Choice (MAGIC) methodological framework. First, the clinical specialist identified the topic of recommendation and demand for evidence. Then the independent evidence synthesis group performed a systematic review to summarize available evidence and evaluate the evidence using the GRADE approach. Finally, the clinical specialist group produced recommendations for practice through a consensus procedure.The evidenceThe linked systematic review and meta‐analysis included 9422 KOA patients, 61.1% of whom were women. The median mean age was 61.8 years. Compared with no treatment, acupuncture had beneficial effect on KOA in improving the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score (moderate certainty evidence), and WOMAC pain (very low certainty evidence), WOMAC stiffness (low certainty evidence), and WOMAC function (low certainty evidence) subscale scores. Compared with usual care, acupuncture improved WOMAC stiffness subscale score (moderate certainty evidence). Subgroup analyses showed different effects in the improvement of WOMAC total scores by different durations of acupuncture and whether acupuncture combined with NSAIDs, but no difference between manual acupuncture and electroacupuncture was found.Understanding the recommendationsCompared with no treatment, acupuncture is suggested to reduce pain, stiffness, and disfunction in KOA patients, ultimately improving the patient's health status. Acupuncture can be used as an alternative therapy when usual care is ineffective or there are adverse reactions so that patients can no longer continue the treatment. Manual acupuncture or electroacupuncture is suggested for 4–8 weeks to improve the health status of KOA. The patient's values and preferences should be considered when selecting acupuncture for KOA treatment.
Isotopic turnover in polar cod (Boreogadus saida) muscle determined through a controlled feeding experiment
Authors: Ziegler Amanda Fern , Bluhm Bodil A. , Renaud Paul E. , Jørgensen Lis L. 
Published: March 2023

AbstractPolar cod (Boreogadus saida) is an important trophic link within Arctic marine food webs and is likely to experience diet shifts in response to climate change. One important tool for assessing organism diet is bulk stable isotope analysis. However, key parameters necessary for interpreting the temporal context of stable isotope values are lacking, especially for Arctic species. This study provides the first experimental determination of isotopic turnover (as half‐life) and trophic discrimination factors (TDFs) of both δ13C and δ15N in adult polar cod muscle. Using a diet enriched in both 13C and 15N, we measured isotopic turnover times of 61 and 49 days for δ13C and δ15N, respectively, with metabolism accounting for >94% of the total turnover. These half‐life estimates are valid for adult polar cod (>3 years) experiencing little somatic growth. We measured TDFs in our control of 2.6‰ and 3.9‰ for δ13C and δ15N, respectively, and we conclude that applying the commonly used TDF of ~1‰ for δ13C for adult polar cod may lead to misrepresentation of dietary carbon source, while the use of 3.8‰ for δ15N is appropriate. Based on these results, we recommend that studies investigating seasonal shifts in the diet of adult polar cod sample at temporal intervals of at least 60 days to account for isotopic turnover in polar cod muscle. Although isotopic equilibrium was reached by the fish in this study, it was at substantially lower isotope values than the diet. Additionally, the use of highly enriched algae in the experimental feed caused very high variability in diet isotope values which precluded accurate calculation of TDFs from the enriched fish. As a result of the challenges faced in this study, we discourage the use of highly enriched diets for similar experiments and provide recommendations to guide the design of future isotopic turnover experiments.