bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential prognostic and immunotherapeutic value of calponin 1: A pan-cancer analysis
Authors: Zhou Hengli , Ke Junyu , Liu Changhua , Zhu Menglu , Xiao Bijuan , Wang Qi , Hou Rui , Zheng Yueer , Wu Yongqiang , Zhou Xingting , Chen Xinlin , Pan Huafeng 
Published: April 2023

Background: Emerging evidence has suggested a pro-oncogenic role of calponin 1 (CNN1) in the initiation of a variety of cancers. Despite this, CNN1 remains unknown in terms of its effects and mechanisms on angiogenesis, prognosis, and immunology in cancer.Materials and Methods: The expression of CNN1 was extracted and analyzed using the TIMER, UALCAN, and GEPIA databases. Meanwhile, we analyzed the diagnostic value of CNN1 by using PrognoScan and Kaplan–Meier plots. To elucidate the value of CNN1 in immunotherapy, we used the TIMER 2.0 database, TISIDB database, and Sangerbox database. Gene set enrichment analysis (GSEA) was used to analyze the expression pattern and bio-progression of CNN1 and the vascular endothelium growth factor (VEGF) in cancer. The expressions of CNN1 and VEGF in gastric cancer were confirmed using immunohistochemistry. We used Cox regression analysis to investigate the association between pathological characteristics, clinical prognosis, and CNN1 and VEGF expressions in patients with gastric cancer.Results: CNN1 expression was higher in normal tissues than it was in tumor tissues of most types of cancers. However, the expression level rebounds during the development of tumors. High levels of CNN1 indicate a poor prognosis for 11 tumors, which include stomach adenocarcinoma (STAD). There is a relationship between CNN1 and tumor-infiltrating lymphocytes (TILs), and the marker genes NRP1 and TNFRSF14 of TILs are significantly related to CNN1 expression in gastric cancers. The GSEA results confirmed the lower expression of CNN1 in tumors when compared to normal tissues. However, CNN1 again showed an increasing trend during tumor development. In addition, the results also suggest that CNN1 is involved in angiogenesis. The immunohistochemistry results validated the GSEA result (take gastric cancer as an example). Cox analysis suggested that high CNN1 expression and high VEGF expression are closely associated with poor clinical prognosis.Conclusion: Our study has shown that CNN1 expression is aberrantly elevated in various cancers and positively correlates with angiogenesis and the immune checkpoint, contributing to cancer progression and poor prognosis. These results suggest that CNN1 could serve as a promising candidate for pan-cancer immunotherapy.
Mass production of ultra-pure NaI powder for COSINE-200
Authors: Shin KeonAh , Choe JunSeok , Gileva Olga , Iltis Alain , Kim Yena , Kim Yeongduk , Lee Cheolho , Lee Eunkyung , Lee HyunSu , Lee Moo Hyun 
Published: April 2023

COSINE-200 is the next phase experiment of the ongoing COSINE-100 that aims to unambiguously verify the annual modulation signals observed by the DAMA experiment and to reach the world competitive sensitivity on the low-mass dark matter search. To achieve the physics goal of the COSINE-200, the successful production of the low-background NaI(Tl) detectors is crucial and it must begin from the mass production of the ultra-low background NaI powder. A clean facility for mass-producing the pure-NaI powder has been constructed at the Center for Underground Physics (CUP) in Korea. Two years of operation determined efficient parameters of the mass purification and provided a total of 480 kg of the ultra-pure NaI powder in hand. The potassium concentration in the produced powders varied from 5.4 to 11 ppb, and the maximum production capacity of 35 kg per 2 weeks was achieved. Here, we report our operational practice with the mass purification of the NaI powder, which includes raw powder purification, recycling of the mother solution, and recovery of NaI from the residual melt after crystal growth.
Real-world effects of once-daily inhaled steroid (fluticasone furoate) combined with long-acting beta-2 agonist (vilanterol) and long-acting muscarinic antagonist (umeclidinium) on lung function tests of asthma patients in Japan
Authors: Umeda Akira , Shimada Hisato , Yamane Tateki , Mochizuki Taichi , Inoue Yasushi , Tsushima Kenji , Miyagawa Kazuya , Mochida Atsumi , Takeda Hiroshi , Okada Yasumasa , Masaki Katsunori , Matsusaka Masako , Fukunaga Koichi 
Published: April 2023

Background: The Japanese drug use system allowed the once-daily use of inhaled corticosteroid fluticasone furoate (FF) combined with a long-acting beta-2 agonist vilanterol (VI) and a long-acting muscarinic antagonist umeclidinium (UMEC) against asthma on 18 February 2021. We investigated the real-world effects of these drugs (FF/UMEC/VI) mainly on lung function tests.Methods: This was an open-label, uncontrolled, within-group time-series (before-after) study. Prior asthma treatment (inhaled corticosteroid with/without a long-acting beta-2 agonist with/without a long-acting muscarinic antagonist) was switched to FF/UMEC/VI 200/62.5/25 μg. Subjects were evaluated by lung function tests prior to, and 1–2 months after, initiation of FF/UMEC/VI 200/62.5/25 μg. Patients were asked questions regarding the asthma control test and preference for drugs.Results: Overall, 114 asthma outpatients (97% Japanese) were enrolled from February 2021 to April 2022: 104 subjects completed the study. Forced expiratory volume in 1 s, peak flow, and asthma control test score of FF/UMEC/VI 200/62.5/25 μg-treated subjects were significantly increased (p &lt; 0.001, p &lt; 0.001, and p &lt; 0.01, respectively). In contrast with FF/VI 200/25 μg, instantaneous flow at 25% of the forced vital capacity and expiratory reserve volume were significantly increased by FF/UMEC/VI 200/62.5/25 μg (p &lt; 0.01, p &lt; 0.05, respectively). Sixty-six percent of subjects declared they wanted to continue FF/UMEC/VI 200/62.5/25 μg in the future. Adverse effects, mainly local, were seen in 30% of patients, but no serious adverse effects were seen.Conclusion: Once-daily FF/UMEC/VI 200/62.5/25 μg was effective against asthma without serious adverse events. This is the first report that demonstrated FF/UMEC/VI dilated peripheral airways using lung function tests. This evidence on drug effects may improve our understanding of pulmonary physiology and the pathophysiology of asthma.
Foliar application of putrescine alleviates terminal drought stress by modulating water status, membrane stability, and yield- related traits in wheat (Triticum aestivum L.)
Authors: Wasaya Allah , Rehman Iqra , Mohi Ud Din Atta , Hayder Bin Khalid Muhammad , Ahmad Yasir Tauqeer , Mansoor Javaid Muhammad , El-Hefnawy Mohamed , Brestic Marian , Rahman Md Atikur , El Sabagh Ayman 
Published: April 2023

Drought stress is one of the major limitations to the growth and yield productivity of cereal crops. It severely impairs the early growing and grain -filling stages of wheat. Therefore, cost- effective and eco-friendly approaches for alleviating drought stress in cereal crops are in high demand. Polyamines, such as putrescine, have a significant effect on improving crop yield under drought- stress conditions. Therefore, the current study was executed with the aim of exploring the significance of putrescine in alleviating drought stress and improving yield- related traits in wheat. Two distinct wheat cultivars (Fakhar-e-Bhakkar and Anaj-2017) were treated with the foliar application of different concentrations (control, 0.5, 1.0, and 1.5 PPM) of putrescine (put) under two moisture conditions (well- watered and terminal drought stress). The results demonstrate that the imposition of terminal drought stress significantly reduces different physiological and yield- related traits of both wheat cultivars. The reduction of relative water content (RWC%), membrane stability index (MSI), leaf area, tillers per plant, biomass yield, number of spikelets per spike, 100-grain weight, grain yield per plant, and straw yield was greater in Anaj-2017 than in Fakhar-e-Bhakkar cultivar. The results further explain that the foliar application of increased concentrations of putrescine from 0.0 to 1.0 PPM gradually improved physiological and yield traits, whereas these traits declined with the application of putrescine at the highest dose (1.5 PPM). The exogenous application of 1.0 PPM putrescine improved the relative water content (19.76%), specific leaf area (41.47%), and leaf area ratio (35.84%) compared with the controlled treatment. A higher grain yield (28.0 g plant-1) and 100-grain weight (3.8 g) were obtained with the foliar application of 1.0 PPM putrescine compared with controlled treatments. The findings of this study confirm the protective role of putrescine against terminal drought stress. It is therefore recommended to use putrescine at a concentration of 1.0 PPM, which could help alleviate terminal drought stress and attain better wheat yield.
Re-shaping pruning improves the dynamic response of centuries-old olive trees to branch-shaker vibrations application
Authors: Camposeo Salvatore , Vicino Francesco , Vivaldi Gaetano Alessandro , Pascuzzi Simone 
Published: April 2023

IntroductionThe Mediterranean basin is home to centuries-old large olive trees; high-vigor cultivars are widespread, with training forms poorly adapted to mechanical harvesting by trunk/branch shakers. The significant quantity of leaves, the considerable tree height, and the presence of multiple dichotomous hanging branches reduce the transmission of vibrations applied by the branch-shaker machines. Thus, re-shaping pruning may improve the performance of this modern mechanical harvesting method by focusing on removing both the hanging branches and those forming dichotomies. The goal of this study was to evaluate the dynamic responses of large-sized olive trees to pruning (or not) through various field tests under different excitation forces. We hypothesized that more rational pruning could significantly increase vibration transmissions.MethodsTo assess the transmission of vibrations, tests were conducted before and after the pruning on representative trees. Tri-axial accelerometers packed in a small titanium housing were used. Trees were assessed before and after the re-shaping pruning. This study reports the first data about the dynamic behavior of centuries-old tree skeletons, in the context of very large-sized olive trees, while taking into account the effects of two different vibrations application modes: a realistic one represented by the system vibration head-tree, originated when the gripper of a shaking machine wrapped and fastened the main branch of the olive trees, and a more speculative one, represented by a single impulse of a short-duration force originated by a hammer.ResultsAfter pruning, spectral density increased 10 fold in the tertiary branches of pruned trees (ranging 1.0–10 m s−2) compared to that of not-pruned ones (ranging 0.1–1.0 m s−2) at frequency &gt;50 Hz under vibration excitation. Moreover, vibrational decay times (120–150 ms) and amplitude (&gt;10−1 m s−2) were higher under single-impulse excitation.DiscussionA more rational pruning applied to ancient large-sized olive trees significantly increased the vibration transmission under both impulse and vibratory excitation forces, without affected their typical “look”. Moreover, these insights are helpful in turn in achieving maximum fruit-removal efficiency. These insights could be applied to various horticultural conditions which would improve the economic sustainability of monumental olive trees, a key portion of the Mediterranean landscape and cultural heritage.
Trans-cinnamaldehyde-related overproduction of benzoic acid and oxidative stress on Arabidopsis thaliana
Authors: López-González David , Ferradás Yolanda , Araniti Fabrizio , Graña Elisa , Hermida-Ramón José M. , González María Victoria , Teijeira Marta , Rey Manuel , Reigosa Manuel J. , Sánchez-Moreiras Adela M. 
Published: April 2023

IntroductionTrans-cinnamaldehyde is a specialised metabolite that naturally occurs in plants of the Lauraceae family. This study focused on the phytotoxic effects of this compound on the morphology and metabolism of Arabidopsis thaliana seedlings.Material and methodsTo evaluate the phytotoxicity of trans-cinnamaldehyde, a dose-response curve was first performed for the root growth process in order to calculate the reference inhibitory concentrations IC50 and IC80 (trans-cinnamaldehyde concentrations inducing a 50% and 80% inhibition, respectively). Subsequently, the structure and ultrastructure of the roots treated with the compound were analysed by light and electron microscopy. Based on these results, the following assays were carried out to in depth study the possible mode of action of the compound: antiauxinic PCIB reversion bioassay, determination of mitochondrial membrane potential, ROS detection, lipid peroxidation content, hormone quantification, in silico studies and gene expression of ALDH enzymes.ResultsTrans-cinnamaldehyde IC50 and IC80 values were as low as 46 and 87 μM, reducing the root growth and inducing the occurrence of adventitious roots. At the ultrastructural level, the compound caused alterations to the mitochondria, which were confirmed by detection of the mitochondrial membrane potential. The morphology observed after the treatment (i.e., appearance of adventitious roots) suggested a possible hormonal mismatch at the auxin level, which was confirmed after PCIB bioassay and hormone quantification by GC-MS. The addition of the compound caused an increase in benzoic, salicylic and indoleacetic acid content, which was related to the increased gene expression of the aldehyde dehydrogenase enzymes that can drive the conversion of trans-cinnamaldehyde to cinnamic acid. Also, an increase of ROS was also observed in treated roots. The enzyme-compound interaction was shown to be stable over time by docking and molecular dynamics assays.DiscussionThe aldehyde dehydrogenases could drive the conversion of trans-cinnamaldehyde to cinnamic acid, increasing the levels of benzoic, salicylic and indoleacetic acids and causing the oxidative stress symptoms observed in the treated seedlings. This would result into growth and development inhibition of the trans-cinnamaldehyde-treated seedlings and ultimately in their programmed-cell-death.
Strength Back – A qualitative study on the co-creation of a positive psychology digital health intervention for spinal surgery patients
Authors: Van Der Horst Annemieke Y. , Bohlmeijer Ernst T. , Schreurs Karlein M. G. , Kelders Saskia M. 
Published: April 2023

IntroductionSpinal surgery patients often experience pain as well as stress, anxiety or even depression before surgery, highlighting the need for better mental preparation before undergoing surgery. Acceptance and Commitment Therapy and positive psychology have proven effective in coping with chronic pain and providing long-term skills that enhance psychological flexibility and mental well-being.The aim of this study is to develop a digital intervention (app) based on Acceptance and Commitment Therapy and positive psychology in co-creation with all stakeholders, including patients and professionals. The aim of the intervention is to increase psychological flexibility and positive skills of spinal surgery patients to promote long-term resilience.Materials and methodsIn this qualitative study, individual, semi-structured interviews were held with healthcare professionals (N = 9) and spinal surgery patients (N = 12) to identify contextual factors and needs for the app. Subsequently, three focus-group sessions were held with healthcare professionals and newly recruited patients to specify relevant values. Also, a first version of the app, named Strength Back, was developed using a participatory design.ResultsThe interviews confirmed the need for information and digital support to cope with insecurity, anxiety and pain, both before and after surgery. Based on iterative steps in the focus-group sessions, thirteen modules were developed focusing on procedural information, pain education, psychological flexibility and mental well-being.DiscussionThe intervention Strength Back, containing information as well as Acceptance and Commitment Therapy and positive psychology exercises, has the potential to increase psychological flexibility, enhance well-being and improve postoperative recovery after spinal surgery.
Transcultural skills for early childhood professionals
Authors: Radjack Rahmeth , Bossuroy Muriel , Camara Hawa , Touhami Fatima , Ogrizek Anaïs , Rodriguez Juliette , Robin Marion , Moro Marie Rose 
Published: April 2023

ContextTranscultural skills are especially useful for those involved in the perinatal period, when parents and babies must adapt to one another in a setting of migration a long a focus of transcultural clinical practice.ObjectiveThe aim of this article is to provide useful transcultural skills for any health care worker (e.g., psychologists, child psychiatrists, midwives, family doctors, pediatricians, specialized child-care attendants, and social workers) who provide care or support to families during the perinatal period. It highlights the cultural aspects requiring attention in relation to representations of pregnancy, children’s needs, obstetric complications, and postnatal problems. Taking into account the impact of culture on clinical evaluation and treatment can enable professionals to distinguish what involves cultural representations of pregnancy, babies, and sometimes of disease from what is associated with interaction disorders or maternal psychopathology.MethodsAfter explaining the relevance of transcultural clinical practices to provide migrant mothers with better support, we describe 9 themes useful to explore from a transcultural perspective. This choice is based on the transcultural clinical practice in our specialized department.ResultsThe description of these 9 themes is intended to aid in their pragmatic application and is illustrated with short clinical vignettes for specific concepts. We describe situations that are extreme but often encountered in liaison transcultural clinical practice for maternity wards: perinatal mourning with cultural coding, mediation in refusal of care, cultural misunderstandings, situations of complex trauma and of multiple contextual vulnerabilities, and difficulties associated with acculturation.DiscussionThe transcultural levers described here make it possible to limit cultural misunderstandings and to promote the therapeutic alliance. It presupposes the professionals will concomitantly analyze their cultural countertransference and acquire both the knowledge and know-how needed to understand the elements of cultural, political, and social issues needed to develop clinical finesse.ConclusionThis combined theoretical-clinical article is intended to be pedagogical. It provides guidelines for conducting transcultural child psychiatry/psychological interviews in the perinatal period aimed at both assessment and therapy.
The experiences of East Asian dementia caregivers in filial culture: a systematic review and meta-analysis
Authors: Wang Qian , Xiao Xiaohua , Zhang Jin , Jiang Dongdong , Wilson Amanda , Qian Beiran , Song Peige , Yang Qian 
Published: April 2023

BackgroundWith the aging population in East Asia, the prevalence of dementia and the need for patient care is increasing. Family caregivers of people with dementia are at risk of physical and mental health problems. Filial piety culture regulates relationships within East Asian families and effects the well-being and behavior of dementia family caregivers (CGs).ObjectiveTo systematically assess the experience of East Asian dementia caregivers in filial culture. Methods: Electronic databases, including MEDLINE, APA PsycINFO, CINAHL (via EBSCOhost), Web of Science, and Cochrane Library, were searched for relevant studies up to July 2021. Only original articles were included.ResultsThirteen eligible studies were included, of which eight were qualitative and five were quantitative. Meta-analysis showed a negative association (r = −0.18, 95%CI [−0.28, −0.08]) between filial culture and caregiver burden. The quantitative studies identified four themes related to dementia caregivers’ experiences: (1) Recognition and understanding of filial piety as part of cultural identity, (2) Role transitions- from child to CG, (3) Filial piety’s constraints on CGs; (4) CGs’ self-compassion through changing cultural norms of filial practice.ConclusionFilial culture influences the whole process behind caregiving for East Asian dementia caregivers. At the same time, cultural transition has also brought about new connotations and practices to filial culture.
Sociodemographic and behavioral factors associated with controlled hypertension after 9 years of observation of a PURE Poland cohort study
Authors: Zatońska Katarzyna , Basiak-Rasała Alicja , Połtyn-Zaradna Katarzyna , Gaweł-Dąbrowska Dagmara , Wołyniec Maria , Karczewski Maciej , Szuba Andrzej 
Published: April 2023

IntroductionDespite some improvement in awareness and treatment of hypertension, blood pressure control is still below expectations in Poland. The aim of the study was to analyze the secular trend of hypertension prevalence in the PURE Poland cohort study over 9 years of observation and to analyze factors associated with controlled HT.MethodsThe study group consisted of 1,598 participants enrolled in a Prospective Urban and Rural Epidemiological Study (PURE), who participated both in baseline (2007–2010) and 9-year follow-up (2016–2019). Hypertension was ascertained on the basis of (1) self-reported hypertension previously diagnosed by the physician, (2) self-reported anti-hypertensive medication, and/or (3) an average of two blood pressure measurements ≥140 mmHg systolic BP and/or ≥90 mmHg diastolic BP.ResultsThe prevalence of hypertension increased from 69.4% at baseline to 85.9% at 9-year follow-up. The chance of HT was 8.6-fold higher in the oldest vs. the youngest age group [OR 8.55; CI 4.47–16.1]. Male sex increased the chance for hypertension over 3-fold [OR 3.23; CI 2.26–4.73]. Obesity, according to BMI, increased the chance of HT 8-fold [OR 8.01; CI 5.20–12.8] in comparison with normal body weight. Male sex decreased the chance of controlled HT after 9 years [OR 0.68; CI 0.50–0.92]. There was no statistically significant association between controlled HT and age or place of residence. Higher and secondary education increased the chance of controlled HT over 2-fold in comparison with primary education [OR 2.35; CI 1.27–4.34, OR 2.34; CI 1.33–4.11]. Obesity significantly decreased the chance of controlled HT after 9 years in comparison with normal body weight [OR 0.54; CI 0.35–0.83].ConclusionFactors significantly increasing the chance for controlled hypertension after 9 years were female sex, secondary and tertiary education, normal body weight, and avoiding alcohol drinking. Changes in lifestyle, with special emphasis on maintaining normal body weight, should be the basis of prevention and control of HT.