bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive cell surface protein profiling of human mesenchymal stromal cells from peritoneal dialysis effluent and comparison with those from human bone marrow and adipose tissue
Authors: Shi Ganggang , Yang Chong , Zhou Lan , Zong Ming , Guan Qiunong , Roza Gerald da , Wang Hao , Qi Hualin , Du Caigan 
Published: June 2023

Abstract
Peritoneal mesenchymal stromal cells (pMSCs) are isolated from peritoneal dialysis (PD) effluent, and treatment with the pMSCs reduces peritoneal membrane injury in rat model of PD. This study was designed to verify the identity of the pMSCs. pMSCs were grown in plastic dishes for 4 to 7 passages, and their cell surface phenotype was examined by staining with a panel of 242 antibodies. The positive stain of each target protein was determined by an increase in fluorescence intensity as compared with isotype controls in flow cytometrical analysis. Here, we showed that pMSCs predominantly expressed CD9, CD26, CD29, CD42a, CD44, CD46, CD47, CD49b, CD49c, CD49e, CD54, CD55, CD57, CD59, CD63, CD71, CD73, CD81, CD90, CD98, CD147, CD151, CD200, CD201, β2-micoglobulin, epithelial growth factor receptor, human leukocyte antigen (HLA) class 1, and, to a lesser extent, CD31, CD45RO, CD49a, CD49f, CD50, CD58, CD61, CD105, CD164, and CD166. These cells lacked expression of most hematopoietic markers such as CD11b, CD14, CD19, CD34, CD40, CD80, CD79, CD86, and HLA-DR. There was 38.55% difference in the expression of 83 surface proteins between bone marrow (BM)-derived MSCs and pMSCs, and 14.1% in the expression of 242 proteins between adipose tissue (AT)-derived MSCs and pMSCs. The BM-MSCs but not both AT-MSCs and pMSCs express cytokine receptors (IFNγR, TNFI/IIR, IL-1R, IL-4R, IL-6R, and IL-7R). In conclusion, pMSCs exhibited a typical cell surface phenotype of MSCs, which was not the same as on BM-MSCs or AT-MSCs, suggesting that the pMSCs may represent a different MSC lineage from peritoneal cavity.
The impact of the COVID-19 pandemic on treatment for domestic violence injuries: evidence from medical claims
Authors: Gilbert Lauren , Parker Susan , Schechter Lauren 
Published: June 2023

Abstract
Previous studies have observed heterogeneous changes in domestic violence-related 911 calls, police incident reports, and arrests at the onset of the COVID-19 pandemic. In this study, we use a large-scale medical claims database with coverage of over 100 million patients to examine the impact on domestic violence victims’ use of emergency department care for their injuries in the early weeks of the pandemic compared to the corresponding weeks in previous years. We find a 35% decrease in utilization of emergency medical services by domestic violence victims between March and June of 2020. Based on this finding, it is essential to use caution when using medical claims to measure domestic violence in future research covering this time period. Decreases in care utilization also have important implications for the detection, screening, and treatment of domestic violence injuries during future public health crises.
Digital motor biomarkers of cerebellar ataxia using an RGB-depth camera-based motion analysis system
Authors: Suzuki Masahide , Hirano Shigeki , Otte Karen , Schmitz-Hübsch Tanja , Izumi Michiko , Tamura Mitsuyoshi , Kuroiwa Ryota , Sugiyama Atsuhiko , Mori Masahiro , Röhling Hanna M. , Brandt Alexander U. , Murata Atsushi , Paul Friedemann , Kuwabara Satoshi 
Published: June 2023

Abstract
This study aimed to identify quantitative biomarkers of motor function for cerebellar ataxia by evaluating gait and postural control using an RGB-depth camera-based motion analysis system. In 28 patients with degenerative cerebellar ataxia and 33 age- and sex-matched healthy controls, motor tasks (short-distance walk, closed feet stance, and stepping in place) were selected from a previously reported protocol, and scanned using Kinect V2 and customized software. The Clinical Assessment Scale for the Assessment and Rating of Ataxia (SARA) was also evaluated. Compared with the normal control group, the cerebellar ataxia group had slower gait speed and shorter step lengths, increased step width and mediolateral trunk sway in the walk test (all P < 0.001). Lateral sway increased in the stance test in the ataxia group (P < 0.001). When stepping in place, the ataxia group showed higher arrhythmicity of stepping and increased stance time (P < 0.001). In the correlation analyses, the ataxia group showed a positive correlation between the total SARA score and arrhythmicity of stepping in place (r = 0.587, P = 0.001). SARA total score (r = 0.561, P = 0.002) and gait subscore (ρ = 0.556, P = 0.002) correlated with mediolateral truncal sway during walking. These results suggest that the RGB-depth camera-based motion analyses on mediolateral truncal sway during walking and arrhythmicity of stepping in place are useful digital motor biomarkers for the assessment of cerebellar ataxia, and could be utilized in future clinical trials.
Digital Healing: Public Sentiments towards Electronic Health Services in Bangladesh
Authors: Islam Md Robiul , Islam M. Rezaul , Rahman Abdur 
Published: June 2023

Abstract

The study aimed to assess the public perception about the prevalence, satisfaction, and challenges of electronic health (e-health) services in Bangladesh. An online survey with a self-administered structured interview was conducted with 150 respondents to collect general perceptions from both rural and urban people. The study revealed that the prevalence of e-health services was moderate in Bangladesh. During the Covid-19 pandemic, people accessed online vaccine registration (58%), telemedicine (23%), and downloaded Covid test reports (17%). The study did not find association between e-health services and the participants' age, gender, living condition, and marital status based on the results of the Chi-square test and the Mann-Whitney U test (p > 0.05). The Spearman correlation analysis confirmed that individuals were moderately satisfied with their access to e-health services (r = .536), the availability of updated Covid-related information on websites (r = .585), and government advertisements (r = .547). Participants reported major challenges in the form of high internet costs, lack of awareness, insufficient internet speed, and an information gap regarding e-health services in Bangladesh. Findings would be an important guideline to the mass people, health experts and practitioners, policymakers, and government health authorities.
The normalized inelastic displacement spectra for seismic response estimation of a SDOF system with a generalized flag-shaped hysteretic model
Authors: Wu Yifeng , Wang Zijian , Li Aiqun , Zhang Guodong , Fu Jiangdi , Wang Hao 
Published: June 2023

Abstract
In order to develop the inelastic displacement spectra suitable for self-centering structures with flag-shaped hysteretic behavior when subjected to near-fault pulse-like (NFPL) ground motions, the nonlinear time history analysis for a single degree of freedom (SDOF) system with a generalized flag-shaped (FS) hysteresis model is first realized by using Matlab software, a total of 85 NFPL records are selected as the seismic input, the variation ranges of all considered parameters, including the ductility, the vibration period, the energy dissipation coefficient and the post-yield stiffness ratio of the hysteresis model are specified, then the constant-ductility inelastic displacement spectra for different seismic records and different parameters are calculated and statistically processed. It is found the normalization of the natural period and the displacement by the pulse period, namely T/Tp and xm/Tp2, can significantly reduce the variation of displacement spectra. The influence of the post-yield stiffness ratio ranging from 0 to 0.20 on the normalized spectra is slight and can be neglected. Besides, as T/Tp increases, the normalized displacement first increases and then slowly decreases, the normalized period corresponding to the peak displacement is approximately 1. When T/Tp is about less than 0.7, the displacement spectra are positively correlated with the ductility, µ, followed by a negative correlation between them as T/Tp grows larger. Moreover, increasing the energy dissipation coefficient will reduce the displacement response, but when the coefficient is greater than 1, its impact on the displacement is rather small. Finally, the equation of the normalized displacement spectra as a function of T/Tp and µ is proposed, and an adjustment factor is added to account for the influence of the energy dissipation coefficient on the displacement spectra.
Recurrent Neonatal Acute Kidney Injury: Incidence, Predictors, and
Outcomes in the Neonatal Intensive Care Unit
Authors: Vincent Katherine , Rutledge Austin , Laney Zegilor , Newman Jill , Selewski David , Steflik Heidi 
Published: June 2023

Abstract
Objective: Determine recurrent neonatal acute kidney injury (rAKI) incidence, risk factors, and associated outcomes.
Study Design: Single-center retrospective cohort of neonates admitted to the NICU 1/1/20-6/30/21. Comparisons were made between those with no AKI, single episode AKI (sAKI), and rAKI. Multivariable linear and logistic regression models were used to assess associations between rAKI and length of mechanical ventilation (LMV), length of hospitalization stay (LOS), mortality, and hypertension (HTN) at discharge.
Results: The incidence of AKI in the cohort of 869 infants was 19%: 705 (81%) no AKI, 100 (12%) sAKI, 64 (7%) rAKI. Both sAKI and rAKI were independently associated with longer LMV and LOS. sAKI was independently associated with almost 4x higher odds of mortality than rAKI.
Conclusion: In this single center cohort of neonates, sAKI independently predicts mortality, however rAKI is independently associated with increased LMV and LOS suggesting rAKI is clinically important and warrants further study.
Ultrasound-Targeted Microbubble Destruction Promotes PDGF-Primed Bone Mesenchymal Stem Cells Transplantation for Myocardial Protection in Acute Myocardial Infarction of rats
Authors: Sun Zhenxing , Cai Yu , Chen Yihan , Jin Qiaofeng , Zhang Ziming , Zhang Li , Li Yuman , Huang Lei , Wang Jing , Yang Yali , Lv Qing , Han Zhengyang , Xie Mingxing , Zhu Xiangming 
Published: June 2023

Abstract
Background
Ultrasound-targeted microbubble destruction (UTMD) has emerged as a promising strategy for the targeted delivery of bone marrow mesenchymal stem cells (MSCs) to the ischemic myocardium. However, the limited migration capacity and poor survival of MSCs remains a major therapeutic barrier. The present study was performed to investigate the synergistic effect of UTMD with PDGF-BB on the homing of MSCs for acute myocardial infarction (AMI).
Methods
MSCs from male donor rats were treated by PDGF-BB, and a novel microbubble formulation were prepared by a thin-film hydration method. In vivo, MSCs with or without PDGF-BB pretreatment were transplanted by UTMD after inducing AMI in experimental rats. The therapeutic efficacy of PDGF-BB-Primed MSCs on myocardial apoptosis, angiogenesis, cardiac function and scar repair was estimated. In vitro, the effect and molecular mechanism of PDGF-BB on MSCs migration and survival were explored.
Results
The results showed that the biological effects of UTMD increased local levels of SDF-1, which promoted the migration of transplanted MSCs to the ischemic region. Compared with UTMD alone, UTMD combined with PDGF-BB pretreatment significantly increased the cardiac homing of MSCs, which subsequently reduced myocardial apoptosis, promoted neovascularization and tissue repair, and increased cardiac function 30 days after MI. In vitro results showed that PDGF-BB enhanced MSC migration, and protected these cells from H2O2-induced apoptosis. Mechanistically, PDGF-BB pretreatment promoted MSCs migration and inhibited H2O2-induced MSC apoptosis via activation of the PI3K/Akt pathway. Further, crosstalk between PDGF-BB and SDF-1/CXCR4 is involved in the PI3K/AKT signaling pathway.
Conclusion
The present study demonstrated that UTMD combined with PDGF-BB treatment could enhance the homing ability of MSCs, thus alleviating AMI in rats. Therefore, UTMD combined with PDGF-BB pretreatment may offer exciting therapeutic opportunities for strengthening MSC therapy in ischemic diseases.
Awareness of the Public Charge, confidence in knowledge, and the use of public healthcare programs among Mexican-origin Oregon Latino/as
Authors: Lopez Edlyn , Boniface Emily , Díaz-Anaya Sara , Cornejo-Torres Yareli , Darney Blair G 
Published: June 2023

Abstract
Objective.
We describe awareness about the modified “public charge” rule among Oregon’s Mexican-origin Latino/a population and whether concerns about the rule influenced disenrollment from state-funded programs, which do not fall under the public charge.
Methods.
We conducted a cross-sectional survey of adults (ages 18–59) recruited at the Mexican consulate and living in the state of Oregon. Our outcomes were awareness (of the public charge, source of knowledge, and confidence in knowledge of the public charge) and disenrolling self or family members from state-funded public healthcare programs due to concerns about the rule. We describe outcomes and used logistic regression and calculated adjusted probabilities to identify factors associated with awareness of the public charge.
Results.
Of 498 Latino/a respondents, 48% reported awareness of the public charge. Among those who knew about the public charge, 14.6% had disenrolled themselves or family members from public healthcare programs and 12.1% were hesitant to seek care due to concerns about the public charge. Younger respondents had a lower adjusted probability of awareness of the public charge (18–24 years: 15.6% (95% CI 3.1–28.2); 30–39 years 54.9% (95% CI 47.7–62.0). Higher education was associated with a higher adjusted probability of awareness of the public charge; ability to speak English was not associated with awareness of the public charge.
Conclusion.
Our study reveals limited awareness about the public charge among Mexican-origin Oregon Latino/as. Outreach and advocacy are essential to ensure Latino/as know their rights to access available state-funded healthcare programs.
Fertility concerns and outcomes in females with anorectal malformations
Authors: Wehrli Lea A. , Reppucci Marina L. , Woodfield Kelly , Ketzer Jill , Rieck Jared M. , Cooper Emily H. , Torre Luis De La , Peña Alberto , Bischoff Andrea , Alaniz Veronica I. 
Published: June 2023

Abstract
Purpose
To assess fertility concerns and to describe pregnancy outcomes in patients with anorectal malformations (ARM).
Methods
This is an IRB approved, cross-sectional study of patients in the Adult Colorectal Research Registry who completed reproductive health surveys between November 2021 and August 2022. Patients assigned female at birth with age 18 or older and ARM were included.
Results
Sixty-four patients with ARM, age 18 or older, were included. Fertility concerns were reported in 26 (40.6%) patients, 11 of which had seen a fertility specialist, including four who had not yet tried to conceive. Fertility concerns were highest amongst cloaca patients who had not yet tried to conceive (37.5%). 26 (40.6%) patients had tried to conceive, of which 16 (25%) reported fertility problems, most frequently uterine abnormalities and damaged or blocked fallopian tubes. 22 (34.4%) participants were able to conceive and 18 (28.1%) had at least one live birth. Patients with ARM who had concerns of fertility, had better FertiQoL when compared to published reference scores for patients experiencing fertility issues.
Conclusion
Providers should be aware of fertility concerns in patients with ARM. Proactive counseling with referrals to a fertility specialist should be considered in patients who desire future fertility.
Genome-wide transcriptome analysis unravels genetic variants associated with root and biomass-related traits under low phosphorus conditions in Rapeseed (Brassica napus L.)
Authors: Ahmad Nazir , Ibrahim Sani , Kuang Lieqiong , Ze Tian , Wang Xinfa , Wang Hanzhong , Dun Xiaoling 
Published: June 2023

Abstract

Background
Oilseed rape (Brassica napus L.) is a crucial source of edible oil and livestock feeding, and is a promising biofuel crop. The increasing demand for oilseed rape requires strategies to increase yield while retaining quality. Breeding crops with an ideal root system architecture (RSA) for high phosphorus use efficiency (PUE) is an effective way to reduce the use of phosphate fertilizers. However, the genetic mechanisms that underpin PUE in rapeseed remain elusive. To address that, we performed a genome-wide association study (GWAS) in 327 rapeseed accessions to unravel the genetic variations in 13 root and biomass traits under low phosphorus (LP; 0.01mM P+).
Results
All measured traits exhibited significant variations with heritabilities ranging from 0.47 to 0.72, and most traits revealed substantial correlations. GWAS identified 39 significant and 31 suggestive trait-SNP associations that integrated into 11 valid quantitative trait loci (QTL) clusters and explained 4.24–24.43% of the phenotypic variance. Furthermore, RNA-sequencing among high and low PUE lines evaluated the expression levels of candidate genes in root at two-time points. In total, 692, 1076, 648, and 934 differentially expressed genes (DEGs) specific to HP1/LP1, HP1CK/LP1CK, HP2/LP2, and HP2CK/LP2CK, respectively, while 761 and 860 DEGs were found common for HP1/LP1/HP1CK/LP1CK and HP2/LP2/HP2CK/LP2CK, respectively. Twelve genes have been identified as associated with root growth and development under LP stress using an integrated approach of GWAS, weighted co-expression network, and differential expression analysis. In previous studies, six genes (BnaA04g23490D, BnaA09g08440D, BnaA09g04320D, BnaA09g04350D, BnaA09g04930D, BnaA09g09290D) that showed differential expression were identified as promising candidate genes for the target traits.
Conclusion
11 QTL clusters and 12 candidate genes associated with root and development under LP stress were identified in this study. Our study's phenotypic and genetic information may be exploited for genetic improvement of root traits to increase PUE in rapeseed.
An Ensemble Learning Interpretation of Geometric Semantic Genetic Programming
Authors: Dick Grant 
Published: June 2023

Abstract
Geometric semantic genetic programming (GSGP) is a variant of genetic programming (GP) that directly searches the semantic space of programs to produce candidate solutions. GSGP has shown considerable success in improving the performance of GP in terms of program correctness, however this comes at the expense of exponential program growth. Subsequent attempts to address this growth have not fully-exploited the fact that GSGP searches by producing linear combinations of existing solutions. This paper examines this property of GSGP and frames the method as an ensemble learning defining mutation and crossover as examples of boosting and stacking, respectively. The ensemble interpretation allows for simple integration of regularisation techniques that significantly reduce the size of the resultant programs. Additionally, this paper examines the quality of parse tree base learners within this ensemble interpretation of GSGP and suggests that future research could substantially improve the quality of GSGP by examining more effective initialisation techniques. The resulting ensemble learning interpretation leads to variants of GSGP that substantially improve upon the performance of traditional GSGP in regression contexts, and produce a method that frequently outperforms gradient boosting.
Nullomer peptide increases immune cell infiltration and reduces tumor metabolism in triple negative breast cancer mouse model
Authors: Ali Nilufar , Wolf Cody , Kanchan Swarna , Veerabhadraiah Shivakumar R. , Bond Laura , Turner Matthew W. , Jorcyk Cheryl L. , Hampikian Greg 
Published: June 2023

Abstract
Background
Nullomers are the shortest strings of absent amino acid (aa) sequences in a species or group of species. Primes are those nullomers that have not been detected in the genome of any species. 9S1R is a 5-aa peptide derived from a prime sequence that is tagged with 5 arginine aa, used to treat triple negative breast cancer (TNBC) in an in vivo TNBC mouse model. 9S1R is administered in trehalose (9S1R-NulloPT), which enhances solubility and exhibits some independent effects against tumor growth and is thus an important component in the drug preparation.
Method
We examined the effect of 9S1R-NulloPT on tumor growth, metabolism, metastatic burden, necrosis, tumor immune microenvironment, and the transcriptome of aggressive mouse TNBC tumors.
Results
The peptide-treated mice had smaller tumors in the initial phase of the treatment, as compared to the untreated control, and reduced in vivo bioluminescence at later stages, which is indicative of metabolically inactive tumors. A decrease in ex vivo bioluminescence was also observed in the excised tumors of treated mice, but not in the secondary metastasis in the lungs. The treatment also caused changes in tumor immune microenvironment with increased infiltration of immune cells and margin inflammation. The treatment upregulated 365 genes and downregulated 710 genes in tumors compared to the untreated group. Consistent with in vitro findings in breast cancer cell lines, downregulated genes in the treated TNBC tumors include Cellular Metabolic Process Related genes (179), specifically mitochondrial genes associated with TCA cycle/oxidative phosphorylation (44), and translation machinery/ribosome biogenesis genes (45). Among upregulated genes, the Developmental Pathway (13), ECM Organization (12) and Focal Adhesion Related Pathways (7) were noteworthy. We also present data from a pilot study using a bilateral BC mouse model, which supports our findings.
Conclusion
In conclusion, although 9S1R-NulloPT was moderate at reducing the tumor volume, it altered the tumor immune microenvironment as well as the tumor transcriptome, rendering tumors metabolically less active by downregulating the mitochondrial function and ribosome biogenesis. This corroborates previously published in vitro findings.
Efficient Solution of Portfolio Optimization Problems via Dimension Reduction and Sparsification
Authors: Buhler Cassidy K. , Benson Hande Y. 
Published: June 2023

Abstract
The Markowitz mean-variance portfolio optimization model aims to balance expected return and risk when investing. However, there is a significant limitation when solving large portfolio optimization problems efficiently: the large and dense covariance matrix. Since portfolio performance can be potentially improved by considering a wider range of investments, it is imperative to be able to solve large portfolio optimization problems efficiently, typically in microseconds. We propose dimension reduction and increased sparsity as remedies for the covariance matrix. The size reduction is based on predictions from machine learning techniques and the solution to a linear programming problem. We find that using the efficient frontier from the linear formulation is much better at predicting the assets on the Markowitz efficient frontier, compared to the predictions from neural networks. Reducing the covariance matrix based on these predictions decreases both runtime and total iterations. We also present a technique to sparsify the covariance matrix such that it preserves positive semi-definiteness, which improves runtime per iteration. The methods we discuss all achieved similar portfolio expected risk and return as we would obtain from a full dense covariance matrix but with improved optimizer performance.