bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐cell transcriptome sequencing of B‐cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy
Authors: Wang Qing , Sun Ke , Liu Rui , Song Ying , Lv Yafeng , Bi Pingping , Yang Fuying , Li Sijia , Zhao Jiawen , Li Xiuqin , Chen Dong , Mei Jialin , Yang Rirong , Chen Kai , Liu Dequan , Tang Shicong 
Published: August 2023

AbstractBackgroundBreast cancer (BC) is a highly heterogeneous disease, and although immunotherapy has recently increased patient survival in a number of solid and hematologic malignancies, most BC subtypes respond poorly to immune checkpoint blockade therapy (ICB). B cells, particularly those that congregate in tertiary lymphoid structures (TLS), play a significant role in antitumour immunity. However, B‐cell heterogeneity at single‐cell resolution and its clinical significance with TLS in BC need to be explored further.MethodsPrimary tumour lesions and surrounding normal tissues were taken from 14 BC patients, totaling 124,587 cells, for single‐cell transcriptome sequencing and bioinformatics analysis.ResultsBased on the usual markers, the single‐cell transcriptome profiles were classified into various clusters. A thorough single‐cell study was conducted with a focus on tumour‐infiltrating B cells (TIL‐B) and tumour‐associated neutrophils (TAN). TIL‐B was divided into five clusters, and unusual cell types, such as follicular B cells, which are strongly related to immunotherapy efficacy, were identified. In BC, TAN and TIL‐B infiltration are positively correlated, and at the same time, compared with TLS‐high, TAN and TIL‐B in TLS‐low group are significantly positively correlated.ConclusionsIn conclusion, our study highlights the heterogeneity of B cells in BC, explains how B cells and TLS contribute significantly to antitumour immunity at both the single‐cell and clinical level, and offers a straightforward marker for TLS called CD23. These results will offer more pertinent information on the applicability and effectiveness of tumour immunotherapy for BC.
Sc3+ Chloro and Alkyl Complexes Coordinated by Pincer NHC‐Tethered Bis(phenolate) Ligands
Authors: Gurina Galina A. , Markin Alexey V. , Cherkasov Anton V. , Godovikov Ivan A. , Ob'edkov Anatoly M. , Trifonov Alexander A. 
Published: August 2023

AbstractBis(phenolate) ligands with benzimidazole‐2‐ylidene (L1) and tetrahydropyrimidine‐2‐ylidene (L2) linkers proved to be suitable coordination environments for the synthesis of isolable Sc3+ chloro and alkyl complexes. The treatment of Sc(CH2SiMe3)3(THF)2 with equimolar amounts of [L1,2H3]Cl afforded chloro complexes L1,2ScCl(solv)2 (solv=THF, Py) in 76–85 % yields. L1,2ScCl(THF)2 were also prepared by the salt metathesis reactions of ScCl3 with [L1,2]Na2 generated from [L1,2H3]Cl and 3 equiv. of NaN(SiMe3)2 (−40 °C, THF) and isolated in somewhat lower yields (68–73 %). L2ScCl(THF)2 was subjected to the alkylation reaction with LiCH2SiMe3 affording alkyl derivative [L2Sc(CH2SiMe3)]2. This compound can be alternatively prepared by the subsequent reactions of [L2H3]Cl with equimolar amount of NaN(SiMe3)2 and Sc(CH2SiMe3)3(THF)2. In the dimeric alkyl compound [L2Sc(CH2SiMe3)]2, one of the phenoxide groups of the dianionic ligand is coordinated to one scandium center, while the second one features μ‐bridging coordination with two metal centers.
Pre‐deacidification of hazelnut oil by short‐path distillation: Changes in 3‐chloropropane‐1,2‐diol esters, glycidyl esters, free fatty acids, tocopherols, and diglycerides
Authors: Kantekin‐Erdogan Meryem Nur , Emektar Kubra , Vardin Asli Yildirim , Ozdikicierler Onur , Tekin Aziz 
Published: August 2023

AbstractPerforming pre‐deacidification using short‐path distillation at high temperatures and vacuum levels is a general application for fats and oils with high free fatty acids (FFA) to reduce their FFA contents to reasonable levels before steam distillation or neutralization. This study aims to determine the changes in 3‐chloropropane‐1,2‐diol esters (3‐MCPDE), glycidyl esters (GE), and diglyceride (DG) as well as the losses in tocopherols during this process. A bleached hazelnut oil having 12.10% FFA content was distilled using a molecular distillation unit at 200 °C, 220 °C, and 240°C temperatures and 0.25, 0.5, 1, and 2 mbar absolute pressures. The target was to reduce FFA content to less than 2% with minimum tocopherol loss. The results showed that considerable removals in FFA and GE contents were achieved by increasing the temperature and vacuum, while fewer decreases were detected in 3‐MCPDE and DG concentrations. Tocopherols were dramatically decreased by temperature rising, therefore, the lowest temperature (200°C) and absolute pressures (0.25–0.5 mbar) might be suitable for pre‐deacidification of hazelnut oil, where the distilled oil contained 1.78%–1.90% FFA, 243.11–286.76 mg kg–1 tocopherol, 0.50–0.55 mg kg–1 3‐MCPDE, 0.03–0.05 mg kg–1 GE and 4.00–4.22 mg kg–1 DG.Practical Application: Vegetable oils with high free fatty acid (FFA) contents are generally subjected to physical refining to avoid considerable refining loss. However, high temperatures applied to vegetable oils during physical refining result in the formation of 3‐MCPDE&GE, and the loss of some nutrients, such as tocopherols. These adverse effects can be reduced through application of lower temperatures and shorter time during physical refining. This can be applied after the FFA contents of the oils are decreased to reasonable levels (less than 2%) by distillation (pre‐deacidification) before the deodorization. Although this technique has been implemented in many plants, there is almost no information on the effects of applied conditions on 3‐MCPDE and GE and some other minor constituents. This manuscript explains the changes in these compounds during the pre‐deacidification of hazelnut oil by molecular distillation. Suitable distillation conditions were also suggested, which would be very useful for industrial refining plants.
Spatial optimization of integrated energy system including hydrogen electrolysis center based on mixed integer second‐order cone programming
Authors: Xiaoxin Zhang , Qigong Chen , Yuan Ge 
Published: August 2023

AbstractHydrogen energy is playing an increasingly important role in integrated energy systems (IES) due to its multiple uses. Green hydrogen can be produced from electricity generated from renewable energy, reducing carbon emissions and environmental pollution. Previous work usually only considers electric vehicles (EVs) as flexible loads, and there are few studies on the production and application process of green hydrogen. In this paper, hydrogen electrolysis centers (HECs) and EVs are simultaneously schedulable loads, integrated into IES, and optimized. The distribution network model is established, and the optimization goal is to minimize network loss while ensuring the safe operation of the power grid. Through the power flow optimization method of mixed‐integer second‐order cone programming, the space optimization scheduling of EVs in the IES is carried out, and the optimal location of the HEC is reasonably planned and set. A simulation instance of IEEE 33 nodes is used to verify the proposed method. The final results show that this method can minimize network loss in the range of safe operation of the system for the spatial scheduling of electrolytic hydrogen centers and EVs. At the same time, it can also provide a reference for HEC addressing.
Renewable energy sources‐based hybrid microgrid system for off‐grid electricity solution for rural communities
Authors: Ali Majid , Riaz Mohsin , Koondhar Mohsin Ali , Akram Muhammad Sarfraz , Guerrero Josep M. , Vasquez Juan C. , Khan Baseem 
Published: August 2023

AbstractRural electrification is a crucial component of meeting sustainable development goals. In Pakistan, load shedding and power outages are frequent. According to the current data, Pakistan is experiencing a shortfall of power between 6000 and 6500 MW. Microgrid technology has the potential to provide a solution to this problem in an efficient and low‐cost manner. This paper proposes the development of a hybrid microgrid system (HMGS) for rural communities. For that purpose, a technological analysis of the HMGS system for rural electrification is performed. Solar photovoltaic (PV) and wind resource‐based renewable energy systems are considered in this work for the electrification of rural areas of Pakistan. A hybrid PV/wind system is designed using MATLAB software. Simulation results show that a 230‐V sinusoidal output voltage has been produced by the proposed model. The advantage of this model is that it minimizes the impact of transients and provides a sinusoidal output waveform.
A parametric numerical study on the performance of polymer electrolyte membrane fuel cell with intermediate‐blocked interdigitated flow field designs
Authors: Bagherighajari Fatemeh , Moradi Bilondi Abbas , Abdollahzadehsangroudi Mohammadmahdi , Hamrang Ali , Páscoa José Carlos 
Published: August 2023

AbstractFlow field design is crucial for achieving higher performance in polymer electrolyte membrane fuel cells (PEMFCs). This study uses a two‐phase, multi‐component, and three‐dimensional model to simulate the performance of PEMFCs that use interdigitated flow field design with intermediate blocks on the cathode side. A detailed parametric study is presented to investigate the effects of various geometric and operational parameters. Of the parameters studied, inlet mass flow rate, relative humidity, and rib width had the greatest impact on cell performance. The results show that increasing the cathode stoichiometric ratio resulted in higher fuel cell performance for blocked interdigitated designs compared to parallel designs. In addition, using cathode channels with higher height values resulted in lower PEMFC performance for all flow fields. Higher values of rib/channel width ratio resulted in lower cell performance due to liquid water accumulation in the rib regions. However, at higher rib/channel width ratios, the positive effect of using interdigitated flow designs was more pronounced. Moreover, at a low relative humidity of RH = 25%, a 10.4% higher performance was obtained for the interdigitated type II compared to cases with RH = 100%, due to more effective over‐rib convection and higher water removal.
Does repetitive transcranial magnetic stimulation improve cognitive function in age‐related neurodegenerative diseases? A systematic review and meta‐analysis
Authors: Miller Amy , Allen Richard J. , Juma Alisha A. , Chowdhury Rumana , Burke Melanie Rose 
Published: August 2023

AbstractObjectiveHigh‐frequency, repetitive transcranial magnetic stimulation (rTMS) targeted over the dorsolateral prefrontal cortex (DLPFC) is widely used in research to promote neuroplasticity and cognitive enhancement. RTMS is a promising intervention to tackle cognitive decline in people with age‐related neurodegenerative diseases. However, there is currently no systematic evidence examining the effects of DLPFC‐targeted, high‐frequency rTMS on cognitive function in this population. The aim of this systematic review was to evaluate the efficacy and moderators of this treatment intervention.MethodsA comprehensive literature search of five electronic databases was performed to identify articles published before October, 2022. Following PRISMA guidelines, the identified articles were screened, data was extracted, and the methodological quality was assessed using the Cochrane tool, Risk of Bias 2. Meta‐analyses were performed using R Studio (v.4.1.2).ResultsSixteen studies involving 474 participants met the inclusion criteria, of which 8 studies measured global cognitive function. The results from the random‐effects meta‐analysis showed rTMS significantly improved global cognitive function relative to control groups shown by a large, significant effect size (g = 1.39, 95% CI, 0.34–2.43; p = 0.017). No significant effects were found between subgroups or for individual cognitive domains.ConclusionsHigh‐frequency rTMS, targeted over the DLPFC, appears to improve global cognitive function in people with age‐related neurodegenerative diseases. However, these results should be interpreted with caution due to the small number of studies included, and high between‐study heterogeneity.
Examination of recent hydroelectric dam projects in Canada for alignment of baseline studies, predictive modeling, and postdevelopment monitoring phases of aquatic environmental impact assessments
Authors: Brown Carolyn J. M. , Noble Bram F. , Munkittrick Kelly R. 
Published: August 2023

Abstract
Environmental impact assessment (EIA) has been widely criticized by the aquatic science community for poorly aligned approaches when selecting endpoints and collecting data during the baseline, predictive modeling, and postdevelopment monitoring phases. If these critical phases of the EIA process are not aligned properly, it can be difficult to evaluate the presence of postdevelopment effects. Examples of the misalignment of these phases include baseline studies failing to measure indicators that are monitored postdevelopment; predictive assessments that do not quantitatively predict conditions or potential impacts postdevelopment; and the failure to identify relevant indicators that may detect effects postdevelopment. For aquatic assessments, understanding how to protect critical ecosystem attributes to satisfy regulatory concerns could help to better align aquatic science monitoring activities across EIA phases. In this article we investigate recent Canadian hydroelectric dam EIAs to evaluate how well recent assessment approaches are meeting these necessary conditions of good aquatic EIA practice through the lens of ecosystem services from a fish's perspective. We found that larger facilities generally had baseline studies and modeling that better supported postdevelopment monitoring, but improvements in structure, linkages, and expectations would better align EIA phases in a manner that would improve assessments and environmental protection. Integr Environ Assess Manag 2024;20:616–644. © 2023 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
New Frontiers in Gas Phase Chemistry and Catalysis. Celebrating the 80th Birthday of Helmut Schwarz
Authors: Apeloig Yitzhak , Shaik Sason 
Published: August 2023

AbstractHelmut Schwarz is a great scientist and a statesman who has made important fundamental contributions to chemistry as a science, and to the globalization of chemistry when he served as the Vice President of the German Research Foundation and subsequently as the President of the Alexander von Humboldt (AvH) Foundation.
Research on equipment safety fault diagnosis method based on multi‐sensor fusion deep network in mechatronics equipment environment
Authors: Wu Dongyan , Wang Mingge 
Published: August 2023

AbstractThe safety performance and stability of mechatronics equipment play an important role in modern industrial production. However, using a single‐sensor signal cannot effectively ensure robustness in complex scenarios. Moreover, the efficient collection and real‐time transmission of information can improve the real‐time performance of fault detection. To this end, this article proposes a novel deep network based on multi‐sensor information fusion for mechatronics equipment fault detection. Firstly, three sensors are used to collect status information. We use the CC2420 model to transmit the collected signals to the server for storage and analysis. Secondly, we designed a multi‐sensor information fusion deep network. To better model local and global features, we introduced convolutional operations and the multi‐head attention mechanism to form the backbone of the network. The results on the self‐built dataset indicate that the proposed model fully utilizes the advantages of multimodal information and deep networks to achieve the optimal fault detection results.
Use of subgroup‐specific hematopoietic stem cell collection efficiencies to improve truncation calculations for large‐volume leukapheresis procedures
Authors: Rogers Kai J. , Mott Sarah L. , Parsons Meredith G. , Schlueter Annette J. 
Published: August 2023

AbstractPurposeA critical component of optimizing peripheral blood (PB) hematopoietic stem cell (HSC) collections is accurately determining the processed blood volume required to collect the targeted number of HSCs. Fundamental to most truncation equations employed to determine this volume is the procedure's estimated collection efficiency (CE), which is typically applied uniformly across all HSC collections. Few studies have explored the utility of using different CEs in subpopulations of donors that have substantially different CEs than the institutional average.MethodsInitial procedures from 343 autologous and 179 allogeneic HSC collections performed from 2018 to 2021 were retrospectively analyzed. Predictive equations were developed to determine theoretical truncation rates in various donor subgroups.ResultsQuantitative variables (pre‐procedure cell counts) and qualitative variables (relatedness to recipient, gender, method of venous access, and mobilization strategy) were found to significantly impact CE. However, much of the variability in CE between donors could not be explained by the variables assessed. Analyses of procedures with high pre‐collection PB cell counts identified lower CE values for these donors' truncation equations which still allow truncation but minimize risk of collecting less CD34+ cells than requested.ConclusionsIndividualized CE does not substantially improve truncation volume calculations over use of a fixed CE and adds complexity to these calculations. The optimal fixed CE varies between autologous and allogeneic donors, and donors with high pre‐collection PB cell counts in either of these groups. This model will be clinically validated and continuously refined through analysis of future HSC collections.
Veno–venous extracorporeal membrane oxygenation（VV–ECMO）導入により救命できた，換気障害による呼吸不全を併発した重症気道損傷の1症例(A case of inhalation injury that caused severe respiratory failure due to ventilation disorder, which was saved by using veno–venous extracorporeal membrane oxygenation (VV–ECMO))
Authors: 薫 堂本 , 均 池側 , 佳裕 中村 , 健志 前島 , 麻里 中嶋 , 和人 櫻本 , 安行 鍬方 
Published: August 2023

要旨受傷早期に気管粘膜の脱落による換気障害から重篤な呼吸不全に陥り，veno–venous extracorporeal membrane oxygenation（VV–ECMO）を使用することで救命することができた気道損傷症例を経験した。症例は57歳の女性。自宅火災で煙を吸い込み受傷した。来院時，意識清明で，顔面・口腔内に煤が付着しており，体幹は4%のII度熱傷のみであった。SpO2は99%（リザーバー付きマスク10L/分酸素投与下），気道狭窄音を聴取した。喉頭鏡で，咽頭・喉頭・気管内に煤が存在し，声門浮腫が確認されたため直ちに気管挿管を実施した。来院20時間後に，急激な気道内圧上昇を伴う換気障害による高度な呼吸性アシデミアを呈したため，VV–ECMOを導入した。気道損傷に対して，ヘパリン，アセチルシステインの吸入を実施し，経日的に気管粘膜の発赤は軽減，分泌物も減少した。呼吸機能も改善し，第5病日にVV–ECMOを離脱し，第10病日には抜管でき，第20病日に転院となった。重症気道損傷による呼吸不全の原因には，受傷早期の浮腫による上気道閉塞だけでなく，遅発性の気管粘膜脱落による気道狭窄・閉塞で生じる換気障害も挙げられる。これらの換気障害への対応は容易ではないが，気管粘膜の脱落は１週間程度で改善することが十分期待できるので，今回のVV–ECMO導入は理にかなっていると考えられた。
胸骨骨折および多発肋骨骨折に対してロッキングスクリューによるプレート固定術を行った前方フレイルチェストの1例(Locking screw plate stabilization of anterior frail chest caused by sternal fracture and multiple rib fractures: Case report)
Authors: 剛 眞喜志 , 薫 原田 , 晋太郎 竹内 , 夏実子 須田 , 敏明 矢野 , 一博 志賀 , 達也 早川 
Published: August 2023

要旨　フレイルチェストは多発肋骨骨折や胸骨骨折によって生じたフレイルセグメントによって胸郭が不安定になり，奇異呼吸を呈して呼吸不全を生じる病態である。陽圧呼吸によるinternal pneumatic stabilizationが標準的治療で，外科的固定術を要する症例は稀である。症例は55歳の男性。交通事故によって多発外傷を受傷し，胸骨骨折と多発肋骨骨折による前方フレイルチェストを合併していた。Internal pneumatic stabilizationだけでは呼吸状態が安定しなかったため，胸骨に対してワイヤー固定，および肋骨に対してプレート固定を行った。それでも胸郭運動は不安定で呼吸不全が残存したため，胸骨および肋骨に対してロッキングスクリューによるプレート固定を行い，呼吸状態が安定した。文献による外科的固定術の適応や有効性の検討とともに，2度の外科的治療を要した前方フレイルチェストとして本症例を報告する。
鈍的腹部外傷を契機に肝膿瘍破裂を来した侵襲性肝膿瘍症候群の1例(Invasive liver abscess syndrome associated with ruptured liver abscess secondary to blunt abdominal trauma: a case report)
Authors: 秀将 工藤 , 翔太 前澤 , 亮太 瀬尾 , 岳史 寺田 , 崇 入野田 
Published: August 2023

要旨　鈍的腹部外傷を契機に肝膿瘍破裂を来した侵襲性肝膿瘍症候群の症例を経験した。症例は70歳代の男性で運転中の一過性意識障害により，事故を起こした。CT検査で肝S5深部からS6に造影剤血管外漏出像を伴う日本外傷学会肝損傷分類IIIb型の肝損傷と診断した。緊急動脈塞栓術を施行し止血を得たが，血液検査でCRP 20.68mg/dLと高値であった。第2病日に体温38.5℃の発熱を認め，感染症の合併を疑い，各種培養を採取し抗菌薬を開始した。第4病日に過粘稠性Klebsiella pneumoniaeが検出され，第5病日にCT検査を再検したところ肝S6に肝膿瘍を認めた。受傷後から右季肋部痛が出現し，後方視的に検討すると来院時のCT画像で肝膿瘍破裂の所見を認めたことから，肝膿瘍による敗血症が先行し鈍的腹部外傷を契機に肝膿瘍破裂を来したと判断した。第9病日に経皮的ドレナージ，第14病日に腹腔鏡下洗浄ドレナージを施行した。第23病日にCRP 17.0mg/dLと再上昇を認め，CT検査を再検したところ，胸腰椎移行部に傍椎体膿瘍を認め，侵襲性肝膿瘍症候群による転移性病変と考えた。肝膿瘍破裂は早期ドレナージが重要であり，鈍的肝損傷の診療において，炎症反応高値と非典型的な画像所見を認めた際には肝膿瘍破裂を疑い治療を進める必要がある。
Untargeted metabolomics liquid chromatography‐high resolution mass spectrometry approach for the geographical origin assessment of Italian dehydrated apples
Authors: Sammarco Giuseppe , Dall'Asta Chiara , Suman Michele 
Published: August 2023

AbstractGeographical provenience is nowadays a relevant aspect of the authenticity and the quality of many food commodities. Dehydrated apple cubes/slices represent an ingredient commonly used by food companies for bakery products. However, this apple‐based matrix is not so known and studied from an analytical point of view. In the present work, seven compounds were identified as key molecules to distinguish between Italian and non‐Italian samples, through an untargeted ultrahigh‐pressure liquid chromatography‐high‐resolution mass spectrometry (UHPLC‐HRMS) approach. This methodology was merged with multivariate statistical analysis, and the principal features were studied and identified considering several identification steps. Samples from 2020 and 2021 harvesting campaigns, with partial and total dehydration rates, with or without peel, and from different apple varieties were considered for the study, for a total of 91 samples. Afterward, the same analysis protocol was applied to an external set (n = 12 samples), included in the statistical models, searching for the key compounds identified in the training set. Interesting and significant results underlined the potentiality of the UHPLC‐HRMS technology as a confirmatory strategy for the geographical origin assessment of dehydrated apple commodities.
Cardiac macrophages undergo dynamic changes after coxsackievirus B3 infection and promote the progression of myocarditis
Authors: Dong Jingwei , Lu Jing , Cen Zhihong , Tang Quan , Li Yong , Qin Lin , Yan Yuluan , Lu Feiyu , Wu Weifeng 
Published: August 2023

AbstractAlthough most patients with acute viral myocarditis recover spontaneously, some patients progress to heart failure. Perturbations in innate immunity may partially explain the heterogeneity of clinical outcomes. As the most abundant immune cells in the heart, cardiac macrophages have heterogeneous origins, including embryonic‐derived resident macrophages (ResMϕs) and monocyte‐derived macrophages (MoMFs). However, the time course change and role of cardiac macrophage subsets has not been fully explored. In the present study, we found that BALB/c mice had prolonged MoMF accumulation and low proportions of ResMϕs that could not be restored to normal levels. MoMFs of BALB/c mice generally exhibit an M1‐dominant functional phenotype. Moreover, the preferential depletion of MoMF by a C‐C chemokine receptor type 2 (CCR2) inhibitor resulted in improved acute myocarditis and chronic fibrosis, as well as the recovery of ResMϕs number and reduced CD4+ T cell expansion. Hence, immunomodulatory therapy that targets the balance among cardiac macrophages and modulates their function is expected to prevent the progression of cardiac injury to overt heart failure and improve adverse outcomes.