bluuebunny/crossref_metadata_2025_split huggingface dataset:
The first detection of two Aeromonas strains in mice of the genus Apodemus
Authors: Andriyanov Pavel A. , Kashina Daria D. , Liskova Elena A. , Zhurilov Pavel A. , Tutrina Anastasia I. , Ermolaeva Svetlana A. , Zakharova Olga I. , Blokhin Andrey A. 
Published: March 2023

AbstractAeromonas spp. are gram-negative facultatively anaerobic bacilli recovered mainly from aquatic environments. Aeromonas spp. were reported to be associated with infections primarily in aquatic and to a lesser extent in terrestrial animals as well as in humans. Up-to-date little is known about aeromonads associated with wild animals, especially with rodents. This study reported the first isolation and characterization of two Aeromonas spp. from internal organs of apparently healthy wild rodents Apodemus uralensis and Apodemus flavicollis captured in the wild environment in the European part of Russia. Isolates were identified as A. hydrophila M-30 and A. encheleia M-2 using the multilocus sequence analysis (MLSA) approach. The isolation of the A. encheleia from rodents is the first described case. Both strains demonstrated beta-hemolytic activity towards human erythrocytes. Antimicrobial susceptibility testing showed that both Aeromonas strains were resistant and intermediate to carbapenems and piperacillin-tazobactam, which was caused by the expression of the genus-specific CphA carbapenemases. A. hydrophila M-30 also demonstrated trimethoprim resistant phenotype. This is usually caused by the carriage of the dfrA or dfrB genes in aeromonads which are frequently associated with integron class I. The latter however was absent in both isolates. Our results expand our understanding of possible aeromonad reservoirs and demonstrate the likelihood of the formation of natural foci of Aeromonas infection and a new link in the chain of the spread of antimicrobial resistance as well.
Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
Authors: Shimizu Takahiro , Fujii Takuto , Hanita Keisuke , Shinozaki Ryo , Takamura Yusaku , Suzuki Yoshiro , Kageyama Teppei , Kato Mizuki , Nishijo Hisao , Tominaga Makoto , Sakai Hideki 
Published: March 2023

AbstractBitterness is an important physiological function in the defense responses to avoid toxic foods. The taste receptor 2 family is well known to mediate bitter taste perception in Type II taste cells. Here, we report that the polycystic kidney disease 2-like 1 (PKD2L1) channel is a novel sensor for the bitter aftertaste in Type III taste cells. The PKD2L1 channel showed rebound activation after the washout of quinine, a bitter tastant, in electrophysiological whole-cell recordings of the PKD2L1-expressing HEK293T cells and Ca2+-imaging analysis of Type III taste cells isolated from wild-type PKD2L1 mice. In the short-term two-bottle preference and lick tests in vivo, the wild-type mice avoided normal water while the PKD2L1-knockout mice preferred normal water after they ingested the quinine-containing water. These results may explain the new mechanism of the quinine-triggered bitter aftertaste perception in Type III taste cells.
Comprehensive prognostic effects of systemic inflammation and Insulin resistance in women with breast cancer with different BMI: a prospective multicenter cohort
Authors: Ruan Guo-Tian , Xie Hai-Lun , Hu Chun-Lei , Liu Chen-An , Zhang He-Yang , Zhang Qi , Wang Zi-Wen , Zhang Xi , Ge Yi-Zhong , Lin Shi-Qi , Tang Meng , Song Meng-Meng , Zhang Xiao-Wei , Liu Xiao-Yue , Zhang Kang-Ping , Yang Ming , Yu Kai-Ying , Wang Kun-Hua , Hu Wen , Deng Li , Cong Ming-Hua , Shi Han-Ping 
Published: March 2023

AbstractTo investigate the prognostic value of systemic inflammation and insulin resistance in women with breast cancer with different body mass index (BMI). This multicenter, prospective study included 514 women with breast cancer. Multivariate survival analysis showed that patients with high C-reactive protein (CRP), high CRP to albumin ratio (CAR), high lymphocyte to CRP ratio (LCR), high low-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (LHR), and high triglyceride to high-density lipoprotein cholesterol ratio (TG/HDL-c) were significantly associated with worse prognosis. The mortality rate of patients with both high CAR and high LHR or both low LCR and high LHR were 3.91-fold or 3.89-fold higher than patients with both low CAR and low LHR or both high LCR and low LHR, respectively. Furthermore, the combination of LCR and LHR significantly predicted survival in patients within the high BMI group. The CRP, CAR, LCR, LHR, and TG/HDL-c were associated with poor survival in women with breast cancer. The combination of CAR and LHR or LCR and LHR could better predict the prognostic outcomes of women with breast cancer, while the combination of LCR and LHR could better predict the prognosis of those patients with overweight or obese patients.
Spermidine dietary supplementation and polyamines level in reference to survival and lifespan of honey bees
Authors: Đorđievski Srđana , Vukašinović Elvira L. , Čelić Tatjana V. , Pihler Ivan , Kebert Marko , Kojić Danijela , Purać Jelena 
Published: March 2023

AbstractHoney bee health has been an important and ongoing topic in recent years. Honey bee is also an important model organism for aging studies. Polyamines, putrescine, spermidine and spermine, are ubiquitous polycations, involved in a wide range of cellular processes such as cell growth, gene regulation, immunity, and regulation of lifespan. Spermidine, named longevity elixir, has been most analysed in the context of aging. One of the several proposed mechanisms behind spermidine actions is antioxidative activity. In present study we showed that dietary spermidine supplementation: (a) improved survival, (b) increased the average lifespan, (c) influenced the content of endogenous polyamines by increasing the level of putrescine and spermidine and decreasing the level of spermine, (d) reduced oxidative stress (MDA level), (e) increased the antioxidant capacity of the organism (FRAP), (f) increased relative gene expression of five genes involved in polyamine metabolism, and (g) upregulated vitellogenin gene in honey bees. To our knowledge, this is the first study on honey bee polyamine levels in reference to their longevity. These results provide important information on possible strategies for improving honey bee health by introducing spermidine into their diet. Here, we offer spermidine concentrations that could be considered for that purpose.
The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer
Authors: Zeng Xiangwei , Yao Bing , Liu Jianpeng , Gong Guan-Wen , Liu Ming , Li Jiahuang , Pan Hua-Feng , Li Qixiang , Yang Dongjun , Lu Peifen , Wu Dongliang , Xu Peipei , Chen Bing , Chen Panhai , Zhang Ming , Zen Ke , Jing Jian , Huang David C. S. , Chen Dijun , Jiang Zhi-Wei , Zhao Quan 
Published: March 2023

AbstractGenomic studies have demonstrated a high frequency of genetic alterations in components of the SWI/SNF complex including the core subunit SMARCA4. However, the mechanisms of tumorigenesis driven by SMARCA4 mutations, particularly in colorectal cancer (CRC), remain largely unknown. In this study, we identified a specific, hotspot mutation in SMARCA4 (c. 3721C>T) which results in a conversion from arginine to tryptophan at residue 1157 (R1157W) in human CRC tissues associated with higher-grade tumors and controls CRC progression. Mechanistically, we found that the SMARCA4R1157W mutation facilitated its recruitment to PRMT1-mediated H4R3me2a (asymmetric dimethylation of Arg 3 in histone H4) and enhanced the ATPase activity of SWI/SNF complex to remodel chromatin in CRC cells. We further showed that the SMARCA4R1157W mutant reinforced the transcriptional expression of EGFR and TNS4 to promote the proliferation of CRC cells and patient-derived tumor organoids. Importantly, we demonstrated that SMARCA4R1157W CRC cells and mutant cell-derived xenografts were more sensitive to the combined inhibition of PRMT1 and SMARCA4 which act synergistically to suppress cell proliferation. Together, our findings show that SMARCA4-R1157W is a critical activating mutation, which accelerates CRC progression through facilitating chromatin recruitment and remodeling. Our results suggest a potential precision therapeutic strategy for the treatment of CRC patients carrying the SMARCA4R1157W mutation.
Hydrophobic interaction enables rapid enrichment of volatile metabolites on Au/TiO2 based SERS substrates for ultrasensitive bacteria detection
Authors: Li Junhan , Xu Jingwen , Liu Yundian , Xu Jing , Yang Lingling , Gao Zhida , Song Yan-Yan 
Published: March 2023

Hydrophobic AuNPs coated TiO2 nanotubes are developed as an interference-free SERSsubstrate. The hydrophobic interaction of volatile metabolites with the hydrocarbon moieties on the SERS substrate lead to a rapid and sensitive bacteria sensing.
Improved LED arrangement through outage probability minimization in LiFi communication systems
Authors: Mohammadi Mahmoud , Sadough Seyed Mohammad Sajad 
Published: March 2023

AbstractIn this paper, an improved method to arrange the light emitting diodes (LED) on the ceiling for an indoor visible light communication (VLC) system is proposed. More precisely, a LiFi‐based connection is considered where several LEDs are placed on the ceiling of an office and communicate with several receiver (user) located randomly within the room area. It is proposed to find the optimal location of the LEDs by minimizing the outage probability at the user's location. Both cases of static and mobile users are addressed. First, the closed‐form expression for the outage probability at the user's location is derived. Then, by minimizing the average outage probability, the optimal location of the LEDs is found. The numerical results show that the proposed LED arrangement outperforms the classically used uniform LED arrangement in terms of average outage probability at the receiver and reduces the necessary signal‐to‐noise ratio (SNR) to achieve a target average outage probability by about 3 and 2.2 dB for mobile and static users, respectively. They also show that the performance superiority of the proposed method over classical arrangement remains valid even under large estimation errors.
Interdependencies in HVDC grid protection: Impact of converter parameters and controls on DC fault‐ride‐through capabilities and protection system design
Authors: Düllmann Patrick , Brantl Christina , Klein Christopher , Moser Albert 
Published: March 2023

AbstractFor future multi‐terminal HVDC networks, protection systems based on DC circuit breakers (DCCBs) are envisioned to limit the loss of power transmission towards AC grid(s). Yet, HVDC protection system design is considered a major challenge—especially in a multi‐vendor setup. In particular, protection systems shall enable HVDC converters to ride through DC faults without protective blocking. Several studies have performed sensitivity analyses to determine the DCCB properties and current‐limiting inductor properties that are required to enable converter fault‐ride‐through (FRT), but the HVDC converters themselves have been simplified and kept identical. However, in a multi‐vendor setup, the exact converter design is not known at the time of DC protection planning. Therefore, this paper investigates the impact of different converter properties on the DC‐FRT behaviour, and reveals implications on HVDC protection design. Firstly, analytical approaches are proposed to mathematically derive potential impact factors on the converters’ FRT characteristics, also considering different converter control architectures. Secondly, a comprehensive EMT study demonstrates a significant impact of the converter parameters on the FRT behaviour. Analytical approaches and EMT results are compared with regard to limitations and applicability, such that the paper's findings can support the development of functional requirements for multi‐vendor multi‐terminal HVDC networks.
Demystifying inertial specifications; supporting the inclusion of grid‐followers
Authors: Harrison Sam , Henderson Callum , Papadopoulos Panagiotis N. , Egea‐Alvarez Agusti 
Published: March 2023

AbstractInertial response from grid‐followers (GFLs) is deemed to be “synthetic” due to a slow response. In contrast, grid‐forming (GFM) inertial response is deemed to be faster and therefore “true” and more useful for frequency stability. This paper explores the differences and similarities between an established example of a GFM and a GFL inertial controller by carrying out parametric sweeps at different operating conditions. The analysis aims to assist the ongoing efforts to quantify grid stabilising phenomena, particularly the recent adaptation of the British grid code to incorporate GFM converters. The optimal tuning configurations are identified, showing that some configurations of the GFL can achieve fast inertial provision on strong grids. These configurations are shown to contain the grid frequency as effectively as the GFM, despite the opposing consensus in the literature. The results also highlight the importance of voltage‐source behaviours in determining the initial evolution of grid frequency. Although a blanket inclusion of all GFL inertial configurations is not appropriate, equally, the existing blanket disqualification could limit the assets available to support GFMs (who will certainly be required to stabilise the grid in a fundamental sense) and could inhibit the rate that the net zero transition can occur.
Influence de l’interprofessionnalité sur l’apprentissage du leadership dans le cadre de dispositifs de formation paramédicale recourant à la simulation. Étude LEADERSHIP
Authors: Molle Jérôme , Hidoux Marie-Annick , Bellet Bruno 
Published: March 2023

Contexte : Dans le domaine de la santé, l’importance du facteur humain pour la sécurité des soins est de plus en plus prégnante. Les recherches ont ainsi conduit les équipes soignantes à se questionner sur leur gestion des compétences non techniques. Un leadership efficace est ainsi déterminant pour gérer une situation critique. Dans ces situations, ce leadership est traditionnellement assumé par le médecin. Pourtant, les situations hors présence médicale sont fréquentes. Les infirmières doivent alors assumer ce rôle et ce, dès leur sortie de formation. Lorsque ses caractéristiques sont similaires, le leadership infirmier possède une égale efficacité en situation critique. Les recommandations suggèrent d’utiliser la simulation pour développer et évaluer les compétences non techniques, notamment le leadership, en formation initiale. L’approche interprofessionnelle semble potentialiser le processus d’apprentissage. But : L’objectif de cette étude est de comparer le leadership d’équipes interprofessionnelles et monoprofessionnelles lors de situations de gestion de crise simulées (arrêt cardiaque adulte). Méthodes : Il s’agit d’une étude monocentrique (n = 102), exploratoire et observationnelle, non randomisée contrôlée avec comparaison à groupe historique. Résultats : Les scores de leadership, évalués par l’échelle f-TEAM (french-Team Emergency Assessment Measure), étaient significativement plus élevés dans le groupe interprofessionnel (Δ moyen = −1,21 ; p < 0,05). La théorie de l’apprentissage et l’enseignement contextualisé authentique suggère que l’authenticité de l’environnement interprofessionnel peut influer positivement sur le développement du leadership en situation de gestion de crise simulée. Conclusion : Cette étude semble montrer un impact positif de la simulation interprofessionnelle sur le développement du leadership chez les étudiants en soins infirmiers et les élèves aides-soignants.
The Role of 12/14F Ureteral Access Sheath in Flexible Ureteroscopy for Moderate Nephrolithiasis
Authors: Ozimek Tomasz , Dellas Pauline , Becker Benedikt , Miernik Arkadiusz , Roesch Marie Christine , Merseburger Axel Stuart , Kramer Mario Wolfgang , Wießmeyer Judith Riccarda 
Published: March 2023

Abstract
Introduction: The aim was a retrospective analysis of 12/14F ureteral access sheath (UAS) usage on perioperative outcomes in patients with moderate nephrolithiasis (MN). MN was defined as a maximum of two unilateral kidney stones with a maximum stone diameter of 6–10 mm.
Material and Methods: We conducted a monocentric retrospective univariate and multivariate analysis of flexible ureteroscopies (fURS) performed for MN between 01/2014 and 12/2018.
Results: A total of 402 fURS were performed in patients with urolithiasis; 112 MN cases underwent further analysis. UAS was successfully applied in 33 MN cases [33/112 (29.46%)]. UAS was inserted regardless of the maximum kidney stone diameter and the presence of multiple kidney stones (p > 0.05). Univariate analysis revealed a prolonged median operation time (UAS: 94 min, non-UAS: 74 min, p = 0.04) and median fluoroscopy time (UAS: 75 s, non-UAS: 57.5 s, p = 0.04) in the UAS cohort. These differences were not confirmed on multivariate logistic regression.UAS was not associated with better stone-free rates in either the univariate or multivariate analysis (UAS: 26/33, non-UAS: 61/79, p = 1.0) nor with the occurrence of Clavien-Dindo ≥2 complications (UAS: 3/33, non-UAS: 9/79, p = 0.98) or median length of hospital stay (UAS: 2 days, non-UAS: 2 days, p = 0.169).
Conclusion: We identified no statistical benefits from the usage of 12/14F UAS for MN. As no relevant UAS-associated complications were documented, both strategies (with and without UAS) are feasible.
Comparison of Full Endoscopic Lumbar Diskectomy Using the Transforaminal Approach versus Interlaminar Approach for L5–S1 Lumbar Disk Herniation Treatment: A Meta-Analysis
Authors: Zhu Ming-Tao , Hu Bao-Shan , Chen Chien-Min , Liu Hong-Qi , Lin Guang-Xun 
Published: March 2023

Abstract
Background Numerous studies have examined the clinical effectiveness of transforaminal full endoscopic lumbar diskectomy (T-FELD) and interlaminar full endoscopic lumbar diskectomy (I-FELD) for L5–S1 lumbar disk herniation (LDH), with mixed findings. The goal of this systematic review and meta-analysis was to evaluate the perioperative outcomes, clinical results, and complications of T-FELD and I-FELD to determine their efficacy and safety for treating L5–S1 LDH and to examine the features of complications in depth.
Methods Several databases were searched for articles that matched all of the inclusion criteria. The visual analog scale (VAS) and Oswestry Disability Index (ODI) were used to assess the clinical results. Information on perioperative outcomes and complications was gathered and analyzed.
Results Eight studies with 756 participants were included. There were no significant differences in postoperative bed time (p = 0.44) and hospitalization time (p = 0.49) between T-FELD and I-FELD. When compared with I-FELD, T-FELD was associated with substantially longer fluoroscopy time (p < 0.0001) and operating time (p < 0.0001). There were no significant differences in the preoperative and postoperative VAS and ODI scores between T-FELD and I-FELD. The rates for overall complications, postoperative dysesthesia, postoperative lower extremity pain, incomplete decompression, recurrence, and conversion to open surgery were comparable for T-FLED and I-FELD.
Conclusion T-FELD and I-FELD had equal clinical results and safety for treatment of L5–S1 LDH. Fluoroscopy and operative times were shorter for I-FELD than for T-FELD.
Inside Out Cranioplasty: A Novel Technique for One-Stage Reconstruction of Persistent Scalp Necrosis
Authors: Yokoya Shigeomi , Oka Hideki , Hino Akihiko 
Published: March 2023

Abstract
Background Cranioplasty can be a challenging procedure in certain patients, such as those with scalp necrosis, or when performed after multiple surgical procedures. Herein, we present a patient in whom a part of the skull was placed inside out in a setting where the scalp wound could not be simply sutured because of multiple previous operations.
Methods A 66-year-old patient with a history of multiple craniotomies to resect an intracranial tumor suffered significant skin flap necrosis after harvesting the superficial temporal artery for a bypass intending to ligate the internal carotid artery. He subsequently underwent a one-stage reconstruction surgery, in which the central part of the bone was excised, turned over, and fixed at the inner plate of the bone flap on the outside, and the outer plate on the inside.
Results This technique reduced the skin tension and reduced the epidural dead space, allowing skin flap healing with acceptable cosmetic results.
Conclusion “Inside out cranioplasty” is a valid option for one-stage reconstruction in the cases with scalp necrosis.
Anterior Inter-hemispheric Transcallosal Approach for Resection of Colloid Cyst: A Video Abstract
Authors: Basindwah Sarah , Hawsawi Aysha , Alduhaish Amjad , Ajlan Abdulrazag 
Published: March 2023

Abstract
Background Although histologically benign, colloid cysts have been an entity of interest to neurosurgeons due to the wide array of possible presentations ranging from asymptomatic to high ICP symptoms to sudden death. It is estimated that colloid cysts represent 0.3 to 2% of all brain tumors.1
As they are typically located in the third ventricle, multiple approaches have been adapted and developed for the maximum resection with the least complications given the sensitive anatomy in the area.1
2 The interhemispheric transcallosal approach can be safely performed to fully resect a third ventricle colloid cyst with close to zero recurrence rate and minimal to none permanent deficits.3 This video is an educational illustration of the surgical technique and the related anatomy for the interhemispheric transcallosal approach and how to provide best chances of a benign postoperative course.
Case Description In this surgical video, we present a case of a 20-year-old male, a known case of hypertension and un-controlled diabetes type-1, presented to our hospital with on and off headache, dizziness, and diplopia that gradually progressed. His neurological exam was unremarkable including memory function. CT and MRI scans demonstrated a rounded sharply demarcated lesion at the at the roof of the third ventricle, measuring 1 × 1 cm in size. The patient underwent an anterior inter-hemispheric transcallosal approach, with gross total resection of the cyst. In the follow-up office visit, the patient headache has resolved, and he had no seizures with preserved memory functions.
Conclusion When removal of the colloid cyst is indicated, the interhemispheric transcallosal approach can be performed safely with gross total resection and minimal neurological deficit. This is a surgical demonstration of the transcallosal approach showcasing the surgical corridors and related anatomy.