bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supplementary Figure 1 from Follicle-Stimulating Hormone Receptor as a Target in the Redirected T-cell Therapy for Cancer
Authors: Urbanska Katarzyna , Stashwick Caitlin , Poussin Mathilde , Powell Daniel J. 
Published: April 2023

<p>The expression of 18S used as a internal control (housekeeper). cDNA was constructed from total RNA isolated from 10e5 cells (cultured at 60-70% confluence). PCR performed using intron-spanning primers. Validation criteria included water blanks, and NO RT samples. For human 18SrRNA 5â€™CAGCCACCCGAGATTGAGCA3â€™ and Rev: 5â€™TAGTAGCGACGGGCGGTGTG3â€™ (amplicon 253bp) (Genbank:: NR_003286.2). For mouse ID8 cell line Ribosomal RNA for mouse Eukaryotic small ribosomal subunit [Genbank: NR_003278] primers Fw: 5â€™AGGGGAGAGCGGGTAAGAGA-3â€™	and Rev: 5â€™GGACAGGACTAGGCGGAACA3â€™ were used (amplicon size of 249bp).</p>
Data from Amplified <i>EPOR</i>/<i>JAK2</i> Genes Define a Unique Subtype of Acute Erythroid Leukemia
Authors: Takeda June , Yoshida Kenichi , Nakagawa Masahiro M. , Nannya Yasuhito , Yoda Akinori , Saiki Ryunosuke , Ochi Yotaro , Zhao Lanying , Okuda Rurika , Qi Xingxing , Mori Takuto , Kon Ayana , Chiba Kenichi , Tanaka Hiroko , Shiraishi Yuichi , Kuo Ming-Chung , Kerr Cassandra M. , Nagata Yasunobu , Morishita Daisuke , Hiramoto Nobuhiro , Hangaishi Akira , Nakazawa Hideyuki , Ishiyama Ken , Miyano Satoru , Chiba Shigeru , Miyazaki Yasushi , Kitano Toshiyuki , Usuki Kensuke , Sezaki Nobuo , Tsurumi Hisashi , Miyawaki Shuichi , Maciejewski Jaroslaw P. , Ishikawa Takayuki , Ohyashiki Kazuma , Ganser Arnold , Heuser Michael , Thol Felicitas , Shih Lee-Yung , Takaori-Kondo Akifumi , Makishima Hideki , Ogawa Seishi 
Published: April 2023

<div>Abstract<p>Acute erythroid leukemia (AEL) is a unique subtype of acute myeloid leukemia characterized by prominent erythroid proliferation whose molecular basis is poorly understood. To elucidate the underlying mechanism of erythroid proliferation, we analyzed 121 AEL using whole-genome, whole-exome, and/or targeted-capture sequencing, together with transcriptome analysis of 21 AEL samples. Combining publicly available sequencing data, we found a high frequency of gains and amplifications involving <i>EPOR</i>/<i>JAK2</i> in <i>TP53</i>-mutated cases, particularly those having >80% erythroblasts designated as pure erythroid leukemia (10/13). These cases were frequently accompanied by gains and amplifications of <i>ERG</i>/<i>ETS2</i> and associated with a very poor prognosis, even compared with other <i>TP53</i>-mutated AEL. In addition to activation of the STAT5 pathway, a common feature across all AEL cases, these AEL cases exhibited enhanced cell proliferation and heme metabolism and often showed high sensitivity to ruxolitinib <i>in vitro</i> and in xenograft models, highlighting a potential role of JAK2 inhibition in therapeutics of AEL.</p>Significance:<p>This study reveals the major role of gains, amplifications, and mutations of <i>EPOR</i> and <i>JAK2</i> in the pathogenesis of pure erythroleukemia. Their frequent response to ruxolitinib in patient-derived xenograft and cell culture models highlights a possible therapeutic role of JAK2 inhibition for erythroleukemia with <i>EPOR/JAK2</i>-involving lesions.</p><p><i><a href="https://aacrjournals.org/bloodcancerdiscov/article/doi/10.1158/2643-3230.BCD-3-5-ITI" target="_blank">This article is highlighted in the In This Issue feature, p. 369</a></i></p></div>
Supplemental Table S1 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma
Authors: Maurer Lisa M. , Daley Jessica D. , Mukherjee Elina , Venier Rosemarie E. , Julian Claire M. , Bailey Nathanael G. , Jacobs Michelle F. , Kumar-Sinha Chandan , Raphael Haley , Periyapatna Nivitha , Weiss Kurt , Janeway Katherine A. , Mody Rajen , Lucas Peter C. , McAllister-Lucas Linda M. , Bailey Kelly M. 
Published: April 2023

<p>Benign germline BARD1 variants.</p>
Figure S8 from Combination of a New Oral Demethylating Agent, OR2100, and Venetoclax for Treatment of Acute Myeloid Leukemia
Authors: Kamachi Kazuharu , Ureshino Hiroshi , Watanabe Tatsuro , Yoshida-Sakai Nao , Fukuda-Kurahashi Yuki , Kawasoe Kazunori , Hoshiko Toshimi , Yamamoto Yuta , Kurahashi Yuki , Kimura Shinya 
Published: April 2023

<p>Figure S8. Schedule of HL60 cell xenograft experiments using NOG mice. NOG mice were injected intravenously with HL60 cells. Mice were then treated with OR21 (5.4 mg/kg), Ven (25 mg/kg), and OR21 plus Ven (OR21+Ven) for 21 days since day 7 after transplantation (A). Average mouse body weights (error bars represent standard deviation) for the treatment groups were measured two times per weeks. No weight loss nor severe toxicity were observed in any group during the treatment period (B). Flow cytometric analysis showed tumor burden of human CD45+ (hCD45) leukemic cells in peripheral blood on day 28. The proportion of human CD45-positive cells in the peripheral blood of OR21 plus Ven-treated mice was not lower than that in OR21 or Ven-treated mice (C). Kaplan–Meier analysis showed OR21 plus Ven-treated mice did not exhibit prolonged survival compared to OR21 or Ven-treated mice (p = 0.308 vs. Ven; p = 1.0 vs. OR21; D).</p>
Data from Mavorixafor, an Orally Bioavailable CXCR4 Antagonist, Increases Immune Cell Infiltration and Inflammatory Status of Tumor Microenvironment in Patients with Melanoma
Authors: Andtbacka Robert H.I. , Wang Yan , Pierce Robert H. , Campbell Jean S. , Yushak Melinda , Milhem Mohammed , Ross Merrick , Niland Katie , Arbeit Robert D. , Parasuraman Sudha , Bickley Kris , Yeung Cecilia CS , Aicher Lauri D. , Smythe Kimberly S. , Gan Lu 
Published: April 2023

<div>Purpose:<p>Mavorixafor is an oral, selective inhibitor of the CXCR4 chemokine receptor that modulates immune cell trafficking. A biomarker-driven phase Ib study (NCT02823405) was conducted in 16 patients with melanoma to investigate the hypothesis that mavorixafor favorably modulates immune cell profiles in the tumor microenvironment (TME) and to evaluate the safety of mavorixafor alone and in combination with pembrolizumab.</p>Experimental Design:<p>Serial biopsies of melanoma lesions were assessed after 3 weeks of mavorixafor monotherapy and after 6 weeks of combination treatment for immune cell markers by NanoString analysis for gene expression and by multiplexed immunofluorescent staining for <i>in situ</i> protein expression. Serum samples taken at biopsy timepoints were evaluated for key chemokine and cytokine alterations using the Myriad Rules Based Medicine multiplex immunoassays.</p>Results:<p>Within the TME, mavorixafor alone increased CD8<sup>+</sup> T-cell infiltration, granzyme B signal, antigen presentation machinery, and both tumor inflammatory signature (TIS) and IFNγ gene expression signature scores. Increases in the key serum cytokines CXCL9 and CXCL10 were further enhanced when mavorixafor was combined with pembrolizumab. Adverse events (AE), as assessed by the investigator according to NCI Common Terminology Criteria for Adverse Events (v4.03), related to either mavorixafor or pembrolizumab (≥15%) were diarrhea, fatigue, maculopapular rash, and dry eye. Reported AEs were all ≤ grade 3.</p>Conclusion/Discussion:<p>Treatment with single-agent mavorixafor resulted in enhanced immune cell infiltration and activation in the TME, leading to increases in TIS and IFNγ gene signatures. Mavorixafor as a single agent, and in combination with pembrolizumab, has an acceptable safety profile. These data support further investigation of the use of mavorixafor for patients unresponsive to checkpoint inhibitors.</p>Significance:<p>Despite survival improvements in patients with melanoma treated with checkpoint inhibitor therapy, a significant unmet medical need exists for therapies that enhance effectiveness. We propose that mavorixafor sensitizes the melanoma tumor microenvironment and enhances the activity of checkpoint inhibitors, and thereby may translate to a promising treatment for broader patient populations.</p></div>
Revisiting Apuleius’ De re publica (Fulgentius, Expositio sermonum antiquorum 44)
Authors: Costantini Leonardo 
Published: April 2023

Abstract
This study focuses on a fragment of Apuleius’ lost De re publica preserved in Fulgentius, Expositio sermonum antiquorum 44. After reviewing earlier scholarship on this passage, it is argued that its Plautine vocabulary is consistent with Apuleius’ archaising style, far from being evidence against Apuleius’ paternity. An echo of Aristophanes, Knights 542 is discussed (a tag that later became a political adage), as well as of Plato, Republic 488a-489a and Cicero, Republic 1.11. It is suggested that the fragment might derive from a dialogue somewhat akin to the eponymous ones by Plato and Cicero, or from a speech on moral philosophy.
New evidence on cryptic speciation in soil-inhabiting nematodes, with morphological and molecular characterisation of Xiphinema sangesarense n. sp. (Dorylaimida: Longidoridae) from north-central mountains of Iran
Authors: Poureskandarian Mohammad Ali , Pourjam Ebrahim , Pedram Majid 
Published: April 2023

Summary
A new species of the dagger nematode, genus Xiphinema, was recovered from the rhizospheric soil samples collected from rangelands in Semnan province, north-central Iran, and described based upon both morphological and molecular data. Xiphinema sangesarense n. sp. is characterised by 3.7-4.8 mm long females having 136-154 μm long odontostyle, 74-87 μm long odontophore, two equally developed genital tracts with crystalloid bodies in the tubular part of the uterus, short dorsally convex, ventrally slightly convex tail with a mucron at the end, four juvenile developmental stages and males present, functional. The new species most closely resembles X. azarbaijanense and is regarded as its tentative cryptic form, but was separated from it by reproductive mode, and morphological and molecular differences. Compared to X. afratakhtehense, the second similar species, it could be separated based on morphological differences, especially characteristics of juveniles and molecular data. Furthermore, the new species has close morphology with five know species, namely: X. aequum, X. horvatovicae, X. illyricum, X. macedonicum and X. vuittenezi, in terms of morphological characteristics (mainly structure of uterus and similar female tail), and their differences are discussed. In phylogenetic analyses of the new species using several sequences of two partial large subunit (LSU) D2-D3, and internal transcribed spacer (ITS) sequences, the new species formed a clade with Xiphinema sp. in the LSU tree, and in the ITS tree it formed a clade with X. afratakhtehense. In the cytochrome c oxidase I (COI) gene tree, sequences of the new species occupied a placement inside the clade of several sequences of X. afratakhtehense.
Spatial and Seasonal Variations of Sea Surface Temperature Threshold for Tropical Convection
Authors: Hu Shineng , Xie Shang-Ping , Seager Richard , Cane Mark A. 
Published: April 2023

Abstract
Tropical rainfall variations are of direct societal relevance and drive climate variations worldwide via teleconnections. The convective rainfall tends to occur when sea surface temperature (SST) exceeds a threshold, SSTthr, usually taken to be constant in time and space. We analyze 40-yr monthly observations and find that SSTthr varies by up to 4°C in space and with season. Based on local convective instability, we develop a quantitative theory that largely explains the SSTthr variations using the climatological state of the tropical atmosphere. Although it is often assumed that spatial variations of tropical upper-tropospheric temperature are small and can be neglected, it is shown that lower climatological values favor a lower SSTthr. Similarly, a small increase in climatological surface relative humidity also leads to a decrease in SSTthr, as does a lower climatological air–sea temperature difference. Consequently, efforts to understand and predict natural or forced variations in tropical rainfall must account for, in addition to SST, the temperatures aloft and the near-surface humidity and temperature and requires improved understanding of what controls their distribution in space and time.
Impact of Design Factors for ESA CCI Satellite Soil Moisture Data Assimilation over Europe
Authors: Heyvaert Zdenko , Scherrer Samuel , Bechtold Michel , Gruber Alexander , Dorigo Wouter , Kumar Sujay , De Lannoy Gabriëlle 
Published: April 2023

Abstract
In this study, soil moisture retrievals of the combined active–passive ESA Climate Change Initiative (CCI) soil moisture product are assimilated into the Noah-MP land surface model over Europe using a one-dimensional ensemble Kalman filter and an 18-yr study period. The performance of the data assimilation (DA) system is evaluated by comparing it with a model-only experiment (at in situ sites) and by assessing statistics of innovations and increments as DA diagnostics (over the entire domain). For both assessments, we explore the impact of three design choices, resulting in the following insights. 1) The magnitude of the assumed observation errors strongly affects the skill improvements evaluated against in situ stations and internal diagnostics. 2) Choosing between climatological or monthly cumulative distribution function matching as the observation bias correction method only has a marginal effect on the in situ skill of the DA system. However, the internal diagnostics suggest a more robust system parameterization if the observations are rescaled monthly. 3) The choice of atmospheric reanalysis dataset to force the land surface model affects the model-only skill and the DA skill improvements. The model-only skill is higher with input from the MERRA-2 than with input from the ERA5 reanalysis, resulting in larger DA skill improvements for the latter. Additionally, we show that the added value of the DA strongly depends on the quality of the satellite retrievals and land cover, with the most substantial soil moisture skill improvements occurring over croplands and skill degradation occurring over densely forested areas.
Persistent Disparities in Pediatric Health Care Engagement During the COVID-19 Pandemic
Authors: Phan Thao-Ly T. , Enlow Paul T. , Lewis Amanda M. , Arasteh Kamyar , Hildenbrand Aimee K. , Price Julia , Schultz Corinna L. , Reynolds Victoria , Kazak Anne E. , Alderfer Melissa A. 
Published: April 2023

Objective: The COVID-19 pandemic has disrupted traditional health care, including pediatric health care. We described the impact of the pandemic on disparities in pediatric health care engagement. Methods: Using a population-based cross-sectional time-series design, we compared monthly ambulatory care visit volume and completion rates (completed vs no-show and cancelled visits) among pediatric patients aged 0-21 years in 4 states in the mid-Atlantic United States during the first year of the COVID-19 pandemic (March 2020–February 2021) with the same period before the pandemic (March 2019–February 2020). We used unadjusted odds ratios, stratified by visit type (telehealth or in-person) and sociodemographic characteristics (child race and ethnicity, caregiver primary language, geocoded Child Opportunity Index, and rurality). Results: We examined 1 556 548 scheduled ambulatory care visits for a diverse pediatric patient population. Visit volume and completion rates (mean, 70.1%) decreased during the first months of the pandemic but returned to prepandemic levels by June 2020. Disparities in in-person visit completion rates among non-Hispanic Black versus non-Hispanic White patients (64.9% vs 74.3%), patients from socioeconomically disadvantaged versus advantaged communities as measured by Child Opportunity Index (65.8% vs 76.4%), and patients in rural versus urban neighborhoods (66.0% vs 70.8%) were the same during the remainder of the first year of the pandemic as compared with the previous year. Concurrent with large increases in telehealth (0.5% prepandemic, 19.0% during the pandemic), telehealth completion rates increased. Conclusions: Disparities in pediatric visit completion rates that existed before the pandemic persisted during the pandemic. These findings underscore the need for culturally tailored practices to reduce disparities in pediatric health care engagement.
Translation and adaptation to Spanish of Aberdeen varicose veins questionnaire
Authors: Ibarra Sanchez Gabriela , Ansuategui Vicente Marina , Revuelta Suero Sergio , Cases Perez Cristina , Comanges Yeboles Alejandra , Gonzalez Fajardo Jose Antonio 
Published: April 2023

Objective The purpose of the study is to translate the Aberdeen Varicose Vein Questionnaire (AVVQ) into Spanish and evaluate the feasibility and reliability of the Spanish-translated AVVQ in patients with chronic venous disease. Methods Reliability was assessed by test and retest of the Spanish translated AVVQ in a sample of 77 patients. The questionnaires were answered within a 2-week interval. Results There was a 100% test and retest response. The AVVQ showed 6.5% of missing responses. Cronbach’s alpha was 0.71 indicating an adequate level of internal consistency. Spearman’s rho showed a significant strong association between test and retest scores (rho = 0.84, p < 0.0001). Conclusions The Spanish-translated AVVQ is a reliable tool in our daily clinical practice in order to measure the impact of venous disease in the quality of life.
Risk factors for decreased walking ability in hospitalized patients with aspiration pneumonia
Authors: Yanagita Yorihide , Arizono Shinichi , Tawara Yuichi , Oomagari Masaki , Machiguchi Hikaru , Yokomura Koshi , Katagiri Norimasa , Iida Yuki 
Published: April 2023

Objective To evaluate the factors that influence walking ability in patients hospitalized due to aspiration pneumonia. Methods This retrospective observational study evaluated patients hospitalized with aspiration pneumonia. The primary endpoint was preservation of walking ability. Univariate and multivariate logistic regression analyses were performed with the preservation of walking ability as the dependent variable. Results A total of 143 patients were enrolled in this study. The patients were divided into two groups: those whose walking ability decreased after hospitalization ( n = 61) and those whose walking ability was maintained after hospitalization ( n = 82). Multivariate logistic regression analyses showed that A-DROP (odds ratio [OR] 3.006; 95% confidence interval [CI] 1.452, 6.541; P < 0.01), the Geriatric Nutritional Risk Index (OR 0.919; 95% CI 0.875, 0.960; P < 0.001) and days to initial mobilization (OR 1.221; 95% CI 1.036, 1.531; P < 0.05) were the independent early predictors for preservation of walking ability. Conclusion Nutritional status and early mobilization were important risk factors affecting the maintenance of walking ability in patients hospitalized due to aspiration pneumonia. Thus, a combination of nutrition and early rehabilitation is needed for these patients. Registry of Research Studies involving Human Subjects This study was registered with the University Hospital Medical Information Network Clinical Trial Registry (UMIN 000046923).
Effect of Bone Morphogenetic Protein–2 Combined With Microfracture for Osteochondral Defect of the Talus in a Rabbit Model
Authors: Dang Le Hoang Nam , Tran Nhat Tien , Oh Jong-Sung , Kwon Tae-Young , Lee Kwang-Bok 
Published: April 2023

Background: Osteochondral defects of the talus can be effectively treated using microfracture, which is technically safe, accessible, and affordable. However, fibrous tissue and fibrocartilage comprise the majority of tissue repairs resulting from these procedures. These tissue types lack the mechanical characteristics of native hyaline cartilage and might significantly contribute to the decline in long-term outcomes. Recombinant human-bone morphogenetic protein–2 (rhBMP-2) has been shown to promote matrix synthesis and increase cartilage formation, thus enhancing chondrogenesis in vitro. Purpose: This study aimed to evaluate the treatment ability of combining rhBMP-2 with microfracture in rabbit talus osteochondral defect. Study Design: Controlled laboratory study. Methods: A full-thickness chondral defect (3 × 3 × 2 mm) was constructed in the center talar dome of 24 New Zealand White male rabbits, which were then divided into 4 groups of 6. Each group received the appropriate treatment: group 1 (control; no treatment of defect), group 2 (microfracture treatment), group 3 (rhBMP-2/hydroxyapatite treatment), and group 4 (microfracture combined with rhBMP-2/hydroxyapatite treatment). Animals were sacrificed at 2, 4, and 6 weeks postoperatively. The International Cartilage Regeneration & Joint Preservation Society macroscopic score, which considers the degree of defect repair, the integration to the border zone, and the macroscopic appearance, was used to assess the repaired tissue’s macroscopic appearance. Subchondral bone regeneration in defects was analyzed using micro–computed tomography, and the histological findings were graded using a modified version of the Wakitani scoring system for osteochondral repair. Results: At 2, 4, and 6 weeks, micro-computed tomography analysis revealed that groups 3 and 4 exhibited subchondral bone healing that was more significantly improved compared with groups 1. No sample showed excessive bone growth from the subchondral bone area. According to macroscopic and histological results, group 4 showed higher-quality cartilage and more accelerated cartilage regeneration than the other groups over time. Conclusion: These findings show that osteochondral defect repair in a rabbit talus model could be effectively accelerated and improved by combining rhBMP-2 with microfracture. Clinical Relevance: Using rhBMP-2 in combination with microfracture may enhance the repair of talar osteochondral lesions.