bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mobility and Hip Function Among Geriatric Patients With Displaced Neck of Femur Fractures Treated With Arthroplasty
Authors: Ho Jade Pei Yuik , Wong Amy Yoke Foong , Ong Lik Han , Rutel Ankimtay , Abdullah Sabaruddin , Jaffar Mohd Shahrul Azuan , Bong Chun Haw , Tan Kean Tee , Rao Reuben Prashant , Kamaruddin Faris 
Published: March 2023

Background Neck of femur fractures result in impaired function for older people. Despite surgery, many patients experience a decrease in functional level and poorer health status after the injury. The objectives of this study were (1) to determine the short-term mobility and hip function of geriatric patients who underwent hip replacement surgery for a displaced neck of femur fracture in our local population and (2) to identify factors which affect the functional outcome of these patients. Methods Patients aged 60 years and above, who were admitted for neck of femur fracture from January 2017 to December 2020, and treated surgically with arthroplasty, were included. Information on patient demography, comorbidities, perioperative data, mobility, hip function and complications were retrospectively collected. Outcome measures used were independent ambulation and recovery of pre-fracture mobility at 1 year after surgery while hip function was assessed using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total, pain, stiffness, and physical function scores. Factors associated with these outcomes were analyzed. Results 168 patients with a mean age of 75.2 (SD 8.4) years met the inclusion criteria. 32.1% of patients regained their pre-fracture mobility and 59.6% remained independent 1 year after surgery. Logistic regression analysis identified age, gender, surgical procedure, and time to surgery as significant contributors to recovery of pre-fracture mobility. Older age and increasing requirement for postoperative ambulatory aid resulted in worse WOMAC total and physical function scores. No significant differences were observed in patient-reported hip function between those who had a total hip arthroplasty and those who had a hemiarthroplasty. Conclusion Most geriatric patients with displaced neck of femur fractures did not regain pre-fracture mobility despite surgical treatment with arthroplasty.
Investigation of the Laser Powder Bed Fusion Process of Ti-6.5Al-3.5Mo-1.5Zr-0.3Si Alloy
Authors: Zhang Changchun , Liu Tingting , Liao Wenhe , Wei Huiliang , Zhang Ling 
Published: March 2023

AbstractLaser powder bed fusion (LPBF) is an advanced manufacturing technology; however, inappropriate LPBF process parameters may cause printing defects in materials. In the present work, the LPBF process of Ti-6.5Al-3.5Mo-1.5Zr-0.3Si alloy was investigated by a two-step optimization approach. Subsequently, heat transfer and liquid flow behaviors during LPBF were simulated by a well-tested phenomenological model, and the defect formation mechanisms in the as-fabricated alloy were discussed. The optimized process parameters for LPBF were detected as laser power changed from 195 W to 210 W, with scanning speed of 1250 mm/s. The LPBF process was divided into a laser irradiation stage, a spreading flow stage, and a solidification stage. The morphologies and defects of deposited tracks were affected by liquid flow behavior caused by rapid cooling rates. The findings of this research can provide valuable support for printing defect-free metal components.
The potential role of migratory birds in the transmission of pathogenic Campylobacter species to broiler chickens in broiler poultry farms and live bird markets
Authors: Tawakol Maram M. , Nabil Nehal M. , Samir Abdelhafez , M. Hawash H. , Yonis Ahlam E. , Shahein Momtaz A. , Elsayed Mona M. 
Published: March 2023

Abstract
Background
Campylobacter species (spp.) are one of the most important zoonotic bacteria possessing potential hazards for animal and human health worldwide. Migratory birds are implicated as significant carriers for microbes and a play very important role in the dissemination of Campylobacter to broiler chickens and their environment. The purpose of this investigation was to detect the prevalence, antibiotic resistant patterns, virulence and diversity of pathogenic Campylobacter spp. in 7 migratory bird species (Northern shoveler, Common pochard, Common teal, Northern pintail, Eared Grebe, Great Crested Grebe and Garganey) and broiler chickens that were collected from broiler poultry farms and live bird markets.

Results
The prevalence of Campylobacter was 12.5% (25/200), of which 15% (15/100) was recovered from 5 migratory bird species only and 10% (10/100) from broiler chickens. At the level of migratory birds, eight isolates (53.3%) were Campylobacter jejuni (C. jejuni) and 7 isolates (46.7%) were Campylobacter coli (C. coli) meanwhile, in broiler chickens C. jejuni and C. coli were 50% (5/10) for each. All isolated strains had phenotypic resistance to doxycycline, while all of the isolates were susceptible to amikacin. The multidrug resistance to three, four or five antimicrobial classes was found in 72% (18/25) of the isolated strains. The multiantibiotic resistance index between the examined isolates was 0.22–0.77, with 10 antibiotic resistance patterns. The virulence of isolated Campylobacter strains (from both migratory birds and broiler chicken birds) was detected by targeting the VirB11, ciaB and iam genes which were recorded at 16%, 52% and 100%, respectively. Additionally, 100% and 84% of the antibiotic resistance genes were identified as tetA and BlaOXA-61, respectively.

Conclusions
The results of this study revealed the diversity between all the isolated strains from migratory birds and their similarity to broiler chicken isolates. The findings of the present study highlight the impact of migratory birds visiting Egypt and other countries on pathogenic Campylobacter spp. carrying pathogenic virulence and resistance genes, necessitating the application of biosecurity measures to prevent migratory birds from entering farms during their migration period.
Foliar application of putrescine, salicylic acid, and ascorbic acid mitigates frost stress damage in Vitis vinifera cv. ‘Giziluzum’
Authors: Jalili Ilnaz , Ebadi Ali , Askari Mohammad Ali , KalatehJari Sepideh , Aazami Mohammad Ali 
Published: March 2023

Abstract
Background
Cold stress is an effective factor in reducing production and injuring fruit trees. Various materials, such as salicylic acid, ascorbic acid, and putrescine, are used to alleviate the damage of abiotic stress.

Results
The effect of different treatments of putrescine, salicylic acid, and ascorbic acid on alleviating the damage of frost stress (− 3 °C) to grapes ‘Giziluzum’ was investigated. Frost stress increased the amount of H2O2, MDA, proline, and MSI. On the other hand, it decreased the concentration of chlorophyll and carotenoids in the leaves. Putrescine, salicylic acid and ascorbic acid significantly increased the activities of catalase, guaiacol peroxidase, ascorbate peroxidase, and superoxide dismutase under frost stress. Following frost stress, the grapes treated with putrescine, salicylic acid, and ascorbic acid showed higher levels of DHA, AsA, and AsA/DHA than the untreated grapes. Our results showed that the treatment with ascorbic acid outperformed the other treatments in adjusting frost stress damages.

Conclusion
The use of compounds, such as ascorbic ac id, salicylic acid, and putrescine, modulates the effects of frost stress, thereby increasing the antioxidant defense system of cells, reducing its damage, and stabilizing stable cell conditions, so it can be used to reduce frost damage to different grape cultivars.
ABC-GOALScl score predicts admission to the intensive care unit and mortality of COVID-19 patients over 60 years of age
Authors: Camacho-Moll María Elena , Ramírez-Daher Zayra , Escobedo-Guajardo Brenda Leticia , Dávila-Valero Julio César , Rodríguez-de la Garza Brenda Ludmila , Bermúdez de León Mario 
Published: March 2023

Abstract
Background
One of the risk factors for getting seriously ill from COVID-19 and reaching high mortality rates is older age. Older age is also associated with comorbidities, which are risk factors for severe COVID-19 infection. Among the tools that have been evaluated to predict intensive care unit (ICU) admission and mortality is ABC-GOALScl.

Aim
In the present study we validated the utility of ABC-GOALScl to predict in-hospital mortality in subjects over 60 years of age who were positive for SARS-CoV-2 virus at the moment of admission with the purpose of optimizing sanitary resources and offering personalized treatment for these patients.

Methods
This was an observational, descriptive, transversal, non-interventional and retrospective study of subjects (≥ 60 years of age), hospitalized due to COVID-19 infection at a general hospital in northeastern Mexico. A logistical regression model was used for data analysis.

Results
Two hundred forty-three subjects were included in the study, whom 145 (59.7%) passed away, while 98 (40.3%) were discharged. Average age was 71, and 57.6% were male. The prediction model ABC-GOALScl included sex, body mass index, Charlson comorbidity index, dyspnea, arterial pressure, respiratory frequency, SpFi coefficient (Saturation of oxygen/Fraction of inspired oxygen ratio), serum levels of glucose, albumin, and lactate dehydrogenase; all were measured at the moment of admission. The area under the curve for the scale with respect to the variable of discharge due to death was 0.73 (IC 95% = 0.662—0.792).

Conclusion
The ABC-GOALScl scale to predict ICU admission in COVID-19 patients is also useful to predict in-hospital death in COVID-19 patients  ≥ 60 years old.
Bone marrow mesenchymal stem cell-derived exosomal microRNA-382 promotes osteogenesis in osteoblast via regulation of SLIT2
Authors: Su Hairong , Yang Yulan , Lv Wanchun , Li Xiaoli , Zhao Binxiu 
Published: March 2023

Abstract
Background
Osteoporosis (OP) is a systemic skeletal disorder with increased bone fragility. Human bone marrow mesenchymal stem cells (hBMSCs) have multi-lineage differentiation ability, which may play important roles in osteoporosis. In this study, we aim to investigate the role of hBMSC-derived miR-382 in osteogenic differentiation.

Methods
The miRNA and mRNA expressions in peripheral blood monocytes between persons with high or low bone mineral density (BMD) were compared. Then we collected the hBMSC-secreted sEV and examined the dominant components. The over-expression of the miR-382 in MG63 cell and its progression of osteogenic differentiation were investigated by qRT-PCR, western blot and alizarin red staining. The interaction between miR-382 and SLIT2 was confirmed by dual-luciferase assay. The role of SLIT2 was also confirmed through up-regulation in MG63 cell, and the osteogenic differentiation-associated gene and protein were tested.

Results
According to bioinformatic analysis, a series of differential expressed genes between persons with high or low BMD were compared. After internalization of hBMSC-sEV in MG63 cells, we observed that the ability of osteogenic differentiation was significantly enhanced. Similarly, after up-regulation of miR-382 in MG63 cells, osteogenic differentiation was also promoted. According to the dual-luciferase assay, the targeting function of miR-382 in SLIT2 was demonstrated. Moreover, the benefits of hBMSC-sEV in osteogenesis were abrogated through up-regulation of SLIT2.

Conclusion
Our study provided evidence that miR-382-contained hBMSC-sEV held great promise in osteogenic differentiation in MG63 cells after internalization by targeting SLIT2, which can be served as molecular targets to develop effective therapy.
Specificity protein 1-mediated ACSL4 transcription promoted the osteoarthritis progression through suppressing the ferroptosis of chondrocytes
Authors: He Wen , Lin Xuchao , Chen Kangyao 
Published: March 2023

Abstract
Background
Chondrocytes are the main cell damage type involved in the occurrence and development of osteoarthritis (OA). Ferroptosis has been confirmed to be related to many degenerative diseases. This research aimed to explore the role of Sp1 and ACSL4 in ferroptosis in the IL-1β-treated human chondrocyte cells line (HCCs).

Methods
The cell viability was detected with CCK8 assay. The ROS, MDA, GSH, and Fe2+ levels were assessed with corresponding detecting kits. The Col2a1, Acan, Mmp13, Gpx4 and Tfr1 levels were determined by RT-qPCR assay. Western blot was conducted to evaluate the Acsl4 and Sp1 levels. PI staining was carried out to analyze the cell death. The double luciferase report was conducted to verify the interaction between Acsl4 and Sp1.

Results
The results showed that IL-1β stimulation elevated the LDH release, cell viability, ROS, MDA and Fe2+ levels and declined the GSH levels in the HCCs. Additionally, the mRNA levels of Col2a1, Acan, and Gpx4 were prominently decreased, while Mmp13 and Tfr1 were prominently elevated in the IL-1β stimulated HCCs. Furthermore, Acsl4 protein levels were upregulated in the IL-1β-stimulated HCCs. Both Acsl4 knockdown and ferrostatin-1 treatment neutralized the role of IL-1β in the HCCs. What’s more, Acsl4 was transcriptionally regulated by Specificity protein 1 (Sp1). Sp1 overexpression enhanced the Acsl4 levels and Sp1 knockdown declined it.

Conclusion
Upregulation of Sp1 activates Ascl4 transcription and thus mediates the occurrence of ferroptosis. Hence, Acsl4 may be a therapeutic target for intervention of OA.
Decline in health‐related quality of life and foot and ankle patient reported outcomes measures in patients with haemophilia and ankle haemarthropathy
Authors: Wilkins Richard A. , Siddle Heidi J. , Chapman Graham J. , Horn Elizabeth , Walwyn Rebecca , Redmond Anthony C. 
Published: March 2023

AbstractBackgroundHaemophilia is an X‐linked recessive genetic disorder characterised by bleeding within soft tissue and joints. The ankle is disproportionally affected by haemarthropathy when compared to the elbows and knees; reported as the most affected joints in patients with haemophilia. Despite advances in treatment, patients still report ongoing pain and disability, however, the impact has not been evaluated, nor has the effect on health‐related quality of life (HRQoL) or foot and ankle patient‐reported outcome measures (PROMs). The primary aim of this study was to establish the impact of ankle haemarthropathy in patients with severe and moderate haemophilia A and B. Secondly to identify the clinical outcomes associated with a decline in HRQoL and foot and ankle PROMs.MethodsA cross‐sectional multi‐centre questionnaire study was conducted across 18 haemophilia centres in England, Scotland and Wales with a recruitment target of 245 participants. The HAEMO‐QoL‐A and Manchester‐Oxford Foot Questionnaire (MOXFQ) (foot and ankle) with total and domain scores measured impact on HRQOL and foot and ankle outcomes. Demographics, clinical characteristics, ankle haemophilia joint health scores, multi‐joint haemarthropathy and Numerical Pain Rating Scales (NPRS) of “ankle pain over the past six months” were collected as a measure of chronic ankle pain.ResultsA total of 243 of 250 participants provided complete data. HAEMO‐QoL‐A and MOXFQ (foot and ankle) total and index scores indicated worse HRQoL with total scores ranging from a mean of 35.3 to 35.8 (100 best‐health) and 50.5 to 45.8 (0 best‐health) respectively. NPRS (mean (SD)) ranged from 5.0 (2.6) to 5.5 (2.5), with median (IQR) ankle haemophilia joint health score of 4.5 (1 to 12.5) to 6.0 (3.0 to 10.0) indicating moderate to severe levels of ankle haemarthropathy. Ankle NPRS over six months and inhibitor status were associated with decline in outcome.ConclusionsHRQoL and foot and ankle PROMs were poor in participants with moderate to severe levels of ankle haemarthropathy. Pain was a major driver for decline in HRQoL and foot and ankle PROMs and use of NPRS has the potential to predict worsening HRQoL and PROMs at the ankle and other affected joints.
Contrasting plant growth performance of invasive polyploid and native diploid Prosopis is mediated by the soil bacterial community
Authors: Kaushik Rishabh , Sharma Meesha , Ramana Ch. V. , Sasikala Ch. , Pandit Maharaj K. 
Published: March 2023

AbstractBackgroundSoil microbial communities affect above-ground plant diversity and community composition by influencing plant growth performance. Several studies have tested the effect of soil bacterial microbiome on growth performance of native and invasive plants, but the influence of specific bacterial isolates has not been investigated. Here, we investigated the effects of soil bacterial exclusion by soil sterilization and by inoculation ofStreptomycesrhizobacterial isolates on the growth performance of native and invasiveProsopiscongeners.ResultsPlant growth performance of invasiveP. juliflorawas significantly reduced when grown in sterilized soils, whereas nativeP. cinerariashowed enhanced growth performance in the sterilized soils. When grown in the soil inoculated with the specificStreptomycesisolate fromP. juliflora(PJ1), the growth performance of invasiveP. juliflorawas significantly enhanced while that of nativeP. cinerariaseedlings was significantly reduced. However, inoculation ofP. cinerariaandP. julifloraseedlings withStreptomycesisolate from the rhizosphere of nativeP. cineraria(PC1) had no significant effect on the growth performances either ofP. julifloraorP. cineraria.ConclusionOur study reveals that invasive P. juliflora experiences positive feedback from the non-native soil bacterial community, while the native P. cineraria experiences negative feedback from its soil bacterial community. Our results provide fresh experimental evidence for the enemy release hypothesis, and further our understanding of the contrasting growth-promoting effects of differentially recruited microbial species belonging to the same genus (Streptomyces) in the rhizospheres of alien invasive and native plants.
A study of the oxidation mechanism of the organic pigment indigo in grottoes murals by ozone under dark conditions
Authors: Li Jiaxian , Yu Zongren , Shui Biwen , Yu Yinshui , Tian Yuan , Li Xin , Wang Runquan , Chen Wanping , Zhang Yuerong , Song Kai , Shi Gaofeng , Wang Guoying 
Published: March 2023

Abstract
In this paper, the organic pigments indigo and isatin were detected by high performance liquid chromatography (HPLC) in The Mural of Four Buddhas which is in Cave 3 from the ancient Chinese Tiantishan grottoes (Ming Dynasty, East Slope). By analysing the preservation conditions of the mural and the environmental conditions of the place where the Tiantishan grottoes are located, we speculated that the isatin detected in this mural was mainly produced by the oxidative decomposition of indigo by ozone (O3), rather than by photodegradation of indigo. We have used theoretical calculation software Gaussian09 (G09) and Amsterdam Density Functional (ADF) module in the Amsterdam Modeling Suite (AMS) software to simulate the reaction mechanism of O3 oxidation of indigo, and the end products of the oxidation of the natural plant dye indigo by O3 were identified as isatin, C16H10N2O3 and C8H6NO3 using HPLC, fluorescence spectroscopy and high performance liquid chromatography-mass spectrometry(HPLC–MS). This finding confirmed the accuracy of the mechanism of indigo fading by O3 oxidation. These findings provided a theoretical basis for subsequent research into the derivation of natural organic dyes in the face of increasing O3 pollution and for better protection of valuable historical and cultural heritage such as ancient Chinese grottoes murals.
“Dear future woman of STEM”: letters of advice from women in STEM
Authors: Freedman Gili , Green Melanie C. , Kussman Mia , Drusano Mason , Moore Melissa M. 
Published: March 2023

Abstract
Background
Although a large body of research has identified challenges faced by women in STEM fields and strategies to improve the experience for women in STEM, little of this research has examined which strategies undergraduate women would recommend to their peers. In the current study, undergraduate women in STEM fields (N = 89) wrote letters to younger women in STEM about their experiences. The participants were recruited from a small public liberal arts college and a large public research institution in the United States. Participants were juniors and seniors majoring in engineering, mathematics, computer science, physics, biological or biomedical sciences, and chemistry.

Results
Using thematic analysis, we identified seven types of advice. The participants shared advice about improving academically, forming communities, finding family support, and seeking out women role models. They also provided general words of encouragement and reassured women that everyone struggles, and failure is not indicative of their potential. In some cases, the letters were consistent with themes from prior research; however, other influences that have been studied by quantitative research were not prominent in women’s own advice to their fellow students. For example, although the letters focused on communal themes such as building community, they did not focus on the communal goal of helping others through their careers. Additionally, they highlighted the role of family, which has been relatively neglected in prior work.

Conclusions
The present research highlights which empirically supported theories about retention and success in STEM are reflected in students’ advice to others. These letters also provide insight into which obstacles and solutions were most salient for women students looking back on their undergraduate STEM careers. The women’s letters provide a rich understanding of how women navigate STEM fields and what they would tell future students about persisting in those fields.
Bioengineered extracellular vesicles: future of precision medicine for sepsis
Authors: Areny-Balagueró Aina , Solé-Porta Anna , Camprubí-Rimblas Marta , Campaña-Duel Elena , Ceccato Adrián , Roig Anna , Closa Daniel , Artigas Antonio 
Published: March 2023

AbstractSepsis is a syndromic response to infection and is frequently a final common pathway to death from many infectious diseases worldwide. The complexity and high heterogeneity of sepsis hinder the possibility to treat all patients with the same protocol, requiring personalized management. The versatility of extracellular vesicles (EVs) and their contribution to sepsis progression bring along promises for one-to-one tailoring sepsis treatment and diagnosis. In this article, we critically review the endogenous role of EVs in sepsis progression and how current advancements have improved EVs-based therapies toward their translational future clinical application, with innovative strategies to enhance EVs effect. More complex approaches, including hybrid and fully synthetic nanocarriers that mimic EVs, are also discussed. Several pre-clinical and clinical studies are examined through the review to offer a general outlook of the current and future perspectives of EV-based sepsis diagnosis and treatment.
Genetic mechanisms in generalized epilepsies
Authors: Wang Xiaoqian , Rao Xueyi , Zhang Jia , Gan Jing 
Published: March 2023

AbstractThe genetic generalized epilepsies (GGEs) have been proved to generate from genetic impact by twin studies and family studies. The genetic mechanisms of generalized epilepsies are always updating over time. Although the genetics of GGE is complex, there are always new susceptibility genes coming up as well as copy number variations which can lead to important breakthroughs in exploring the problem. At the same time, the development of ClinGen fades out some of the candidate genes. This means we have to figure out what accounts for a reliable gene for GGE, in another word, which gene has sufficient evidence for GGE. This will improve our understanding of the genetic mechanisms of GGE. In this review, important up-to-date genetic mechanisms of GGE were discussed.