bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variability of McMaster and Mini-FLOTAC techniques for equine strongyle eggs count: Novel analysis by Bland-Altman method
Authors: Cañón-Franco William Alberto , García-Cardona Juan Pablo , López-Orozco Natalia 
Published: August 2023

Abstract
Fecal egg counting techniques in horses are useful in the determination of parasitic loads and anthelmintic efficacy; and have been validated in different geographical scenarios with diverse biological samples. However, until now, there has been no consensus among laboratories, due to the use of statistical analyses with methodological and interpretative limitations, with discrepant results of accuracy and precision for McMaster (MM) and mini-FLOTAC (mF) regarding the more accurate. The study of intra-variability between MM and mF at two different fecal dilutions (5:45 and 4:56), using feces from horses naturally infected with strongyle eggs (n= 130), by duplicate readings, showed high repeatability, minimal intra-laboratorial variability, high accuracy, with low coefficient of variation: mF45[2. 92% ± 0.05], mF56[3.43% ± 0.05], MM56[3.46% ± 0.05] and MM45[3.42% ± 0.05; and high concordance correlation coefficient: mF45 0.97 (0.97 - 0.99, 95%-CI), mF56 0.97 (0.96 - 0.98, 95%-CI, MM56 0.90 (0.87 - 0.93, 95%-CI) and MM45 0.93 (0.90 - 0.95, 95%-CI). Bland-Altman inter-variance analysis (n= 1030) with a single reading, between mF56-mF45 demonstrates a high concordance correlation coefficient of 0.90 (0.87 - 0.93, 95%-CI) and low coefficient of variation (5.9% ± 0.09). On the contrary, a low concordance was recorded for MM56-mF56 and MM56-mF45 concordance correlation coefficient 0. 51 (0.49 - 0.53, 95%-CI) and CCC 0.43 (0.41 - 0.45, 95%-CI), respectively; with negative performance related to egg count and a high coefficient of variation (MM56-mF56 13.2% ± 0.20) and MM56-mF45 (CV 15.3% ± 0.24). The Bland-Altman method allows for the graphical and statistical analysis of repeatability and concordance between diagnostic methods, intuitively, with the use of accuracy and precision indicators, being easy to interpret and compare.
Prevalence of nontuberculous mycobacteria in HIV-infected patients with mycobacterial infections in Beijing, China
Authors: Zhou An , Ma Zichun , Lv Zhibin , Guan Chunshuang , Du Yanni , Wang Wei , Xie Ruming 
Published: August 2023

Abstract
We performed a retrospectively study in a tertiary infectious diseases hospital in Beijing to explore the prevalence and risk factors of NTM among individuals with symptoms suggestive of pulmonary TB. This was a retrospective study of characteristics of patients with suggestive of active TB at Beijing Ditan Hospital. TB accounted for 93.3% of the burden of disease in Beijing cohort of HIV-infected patients with mycobacterial infections, whereas the other 6.7% were due to NTM infections. The receiver operating characteristic curve (ROC) of Albumin combined with CD4/CD8 value for diagnosing active TB from NTM cases was 0.638, and the optimal cut-off values for Albumin and CD4/CD8 were determined as 36.15 g/L and 0.17, respectively. Overall, the most prevalent NTM species associated with pulmonary infections in HIV-infected individuals was M. intracellulare. CD4/CD8 ratio and albumin level indicating their potential as surrogate marker to differentiate TB and NTM infection in HIV-infected population.
The climate-development nexus in coastal Bangladesh to 2050
Authors: Nicholls Robert , Lázár Attila N. , Hutton Craig , Payo Andres , Adams Helen , Haque Anisul , Clarke Derek , Salehin Mashfiqus , Hunt Alistair , Allan Andrew , Adger William , Rahman Munsur 
Published: August 2023

Abstract
Climate change and economic growth are having a profound influence on the integrity of socio-economics and ecology of coastal Bangladesh. In the extreme, there are widespread expectations of inundation and coastal abandonment. However, results from our integrated assessment model (IAM) show that over the next 30 years, development choices might have a stronger influence on livelihoods and economic wellbeing than climate driven environmental change. The IAM simulates the economic development of rural areas by coupling physical models (driven by expectations of climate change) with economic models (informed by a series of policy decisions). This is done using substantial primary, secondary and stakeholder-derived biophysical and socio-economic datasets, together with shocks such as cyclones. The study analyses the future socio-ecological sensitivity to climate change and policy decisions and finds that well managed development is as important as adaptation to mitigate risks, reduce poverty and raise aggregate well-being. This analysis enables decision makers to identify appropriate development pathways that address current social-ecological vulnerability and develop a more resilient future to 2050 and beyond. These policy actions are complementary to climate adaptation and mitigation. Our IAM framework provides a valuable evidence-based tool to support sustainable coastal development and is transferable to other vulnerable delta regions and other coastal lowlands around the world.
Prognostic Accuracy of Quick Sequential Organ Failure Assessment (qSOFA)-lactate for Mortality of Adult with Infection: a Systematic Review and Meta-Analysis
Authors: Lie Khie Chen , Pasaribu Adeline , Shakinah Sharifah , Sinto Robert , Harimurti Kuntjoro , Nainggolan Leonard 
Published: August 2023

Abstract

The high burden of infection mortality worldwide calls for the need for prompt risk assessment of its outcome. This article studied for accuracy of quick Sequential Organ Failure Assessment (qSOFA) combined with lactate measurement in predicting the mortality of adult patients with infection. Literature searching was performed in PubMed/Medline®, CINAHL®, Embase®, CENTRAL®, Proquest®, Scopus®, and hand searching of journals. All studies in searching keywords “infection”, “quick sequential organ failure assessment”, “qsofa”, “lactate” with Medical Subject Headings (MeSH) terms were included. Pooled sensitivity, specificity, and area under the curve (AUC) of qSOFA-lactate accuracy were analysed using the R program. Twenty-six studies comprised of 43,207 adult patients with infection were included in this review. Studies were performed in the emergency department, ward, and intensive care unit, with mortality rates ranging from 3.8% − 53.6%. The pooled sensitivity and specificity of 20 studies were 81.3% (95% CI 72.3%, 87.8%; I2 = 97%, p < 0.01) and 62.3% (95% CI 50.5%, 72.8%; I2 = 100%, p = 0). The AUC of qSOFA-lactate was 0.773 (95% CI 0.74, 0.80). There was no significant difference in accuracy results between septic and non-septic patients. The qSOFA-lactate measurement had good accuracy in predicting mortality in adult infectious patients.
Eversion of upper eyelid during dermolipoma excision: a useful maneuver to avoid postoperative blepharoptosis and dry eye
Authors: Awad Ahmad A. , Aly Momen A. M. 
Published: August 2023

Abstract
Purpose: To describe the benefit for upper eyelid eversion during surgical excision of dermolipoma to avoid postoperative ptosis and dry eye
Method: This retrospective non-comparative interventional clinical study included 32 dermolipoma cases presented to orbit clinic of Assiut university hospital who underwent surgical excision of the lesion. The surgery started with conjunctival incision at the junction between adherent and non-adherent parts followed by dissection between the lesion and tenon capsule. The lateral rectus was hooked and the lesion was separated from the muscle and its covering sheath. The upper lid was then everted using Desmarres retractor and the lesion was pulled downward allowing dissection between the lesion and upper bulbar conjunctiva sparing the fornical conjunctiva which harbor the lacrimal ductules orifices. Then, the deeper part of the lesion is dissected from the adjacent levator under vision. The deep part was dissected from normal orbital tissue using scissors. The bulbar conjunctiva is sutured to the lateral canthus.
Result: Thirty-two dermolipomas in 29 patients were excised. The surgery was successful in all patients without permanent complications. None of our patients suffered from post operative dry eye, blepharoptosis or permanent strabismus. Two patients had temporary limitation of adduction lasting for 1 months.
Conclusion: Everting the upper lid and dissecting the lesion from the underlying levator-muller complex sparing the fornix is an important modification during excision of dermolipoma to avoid postoperative dry eye and blepharoptosis. To the bast of the authors knowledge, this maneuver is first to be reported in the literature.
Vulgar Comments Classification: Comparison between CNN, XGBoost and SVM
Authors: Razzak Razia , Taki Sabiha Tul Omman , Mim Mahfuzur Rahman , Patwary Md Shawkat Hossain , Pavel Monirul Islam 
Published: August 2023

Abstract
In recent years, there has been significant growth in information technology and a disruptive transformation of social media. Popular websites like Facebook, Twitter, and Instagram have allowed users to express themselves through text, photos, and videos. However, these platforms have unfortunately also become breeding grounds for hate speech, abusive behavior, cyberbullying, and anonymous threats. While there have been numerous papers published in this field, they have not fully achieved satisfactory levels of accuracy. This paper aims to address this issue by utilizing natural language processing (NLP) techniques, including convolutional neural networks (CNN), extreme gradient boosting (XGBoost), and support vector machines (SVM), to first segment vulgar comments and then classify them into six different types. The analysis revealed that these algorithms performed closely, but the CNN model demonstrated superior performance with an impressive F1 score of 96.5%, precision score of 98%, and recall value of 95.1%.
Evaluation of Heavy Metal Levels in Chicken Using Indeterministic Analysis: A Study Conducted in Localities of Lahore, Pakistan
Authors: Ahmad Naveed , Afzal Usama , Zafar Ramna , Arslan Muhammad , Riaz Saira , Naseem Shahzad , Naeem Muhammad , Fatima Adeena , Aslam Muhammad 
Published: August 2023

Abstract
The poultry industry is a significant source of animal protein, vitamins, and minerals, particularly through the consumption of chicken meat. A study conducted in Lahore collected 100 samples each of chicken liver, chicken feed and water to investigate the presence of Arsenic and selected heavy metals. The analysis revealed contamination above safe limits, with Copper (Cu), Zinc (Zn), and Cadmium (Cd) being the most prominent metals detected. Neutrosophic statistics proved to be a more informative approach for data analysis. These findings emphasize the importance of monitoring and mitigating heavy metal contamination in the poultry industry to ensure the safety and quality of poultry products.
Doing research in non-specialist mental health services for children and young people: Lessons learnt from a process evaluation of the ICALM (Interpersonal Counselling for Adolescent Low Mood) feasibility Randomised Controlled Trial
Authors: Katangwe-Chigamba Thando , Murdoch Jamie , Wilkinson Paul , Cestaro Viktoria , Seeley Carys , Charami-Roupa Eirini , Clarke Tim , Dunne Aoife , Gee Brioney , Jarrett Sharon , Laphan Andrew , McIvor Susie , Meiser-Stedman Richard , Rhodes Thomas , Shepstone Lee , Turner David , Wilson Jonathan 
Published: August 2023

Abstract
Background
The rising prevalence of adolescent mild depression in the UK and the paucity of evidence-based interventions in non-specialist sectors where most cases present, creates an urgent need for suitable early psychological interventions. Randomised controlled trials (RCTs) are considered the gold standard method for obtaining unbiased estimates of interventions effectiveness. However, the complexity of mental health settings poses great challenges for evaluating interventions using RCTs. Here we report learnings from an embedded process evaluation of the ICALM (Interpersonal Counselling for Adolescent Low Mood) study which sought to feasibility test the delivery of an RCT of Interpersonal Counselling for Adolescents (IPC-A) in non-specialist sectors.
Methods
A mixed methods process evaluation using ethnographic methodology which provides an organising structure for investigating context, structured across macro (e.g., wider policy discourse), meso (e.g., service characteristics) and micro (e.g., on-site trial processes) contextual levels. Methods included questionnaires, semi-structured interviews, focus groups, observations, and documentary analysis. Analysis focused on how mapped contextual features shaped trial implementation.
Results
At a macro-level, delivery of the ICALM study was impacted by the COVID-19 pandemic, structural challenges at referral points and policy implementation challenges. An interaction between enormous demand, a lack of capacity, unclear service specifications and a lack of service coordination at a meso-service level led to low research prioritisation at a micro level thus severely limiting the feasibility of implementing the IPC-A intervention.
Conclusions
Effective implementation of evaluations in non-specialist sectors must consider the integral role of context. Recommendations for researchers include early stakeholder involvement in considerations of appropriate trial designs, effective recruitment strategies and site engagement. The development of a framework for identifying and delivering evidence-based interventions in this setting may facilitate the building of such a research culture and increase success of recruitment to and implementation of RCTs in this setting.
Trial registration
ISRCTN registry, ISRCTN82180413, Registered 31 December 2019
Detection of the antibiotic resistance gene content of normal microbiota Bacteroides, Parabacteroides and Phocaeicola isolates from European countries
Authors: Soki Jozsef , Wybo Ingrid , Baaity Zain , Stefán Gloria , Jeverica Samo , Ulger Nurver , Stingu Catalina-Suzana , Mahmood Bakhtiyar , Burián Katalin , Nagy Elisabeth 
Published: August 2023

Abstract
Background
The Bacteroides fragilis group (BFG) species are the most significant anaerobic pathogens and they are also the most antibiotic resistant anaerobic species. Therefore, surveying their antimicrobial resistance levels and investigating their antibiotic resistance mechanisms is recommended. Since their infections are endogenous and they are important constituents of the intestinal microbiota, the properties of the intestinal strains are also important to follow. The aim of this study was to investigate the main antibiotic gene content of normal microbiota isolates and compare them with the gene carriage of strains isolated from infections.
Results
We detected 14 antibiotic resistance determinants of 184 intestinal BFG strains that were isolated in 5 European countries (Belgium, Germany, Hungary, Slovenia and Turkey) and compared these with values obtained earlier for European clinical strains. Differences were found for antibiotic resistance genes that are considered to be mobile, with higher degrees for cfxA, erm(F) and tet(Q) and with lower degrees for msrSA, erm(B) and erm(G). In addition, a different gene prevalence was found depending on the taxonomical groups, e.g. B. fragilis and NBFB. Some strains with both the cepA and cfiA β-lactamase genes were also detected which is thought to be exceptional since up till now, the B. fragilis genetic divisions were defined by the mutual exclusion of these two genes.
Conclusions
In our study we found that the prevalence of some antibiotic resistance genes detected in the clinical and intestinal BFG strains differ, which we accounted for the differential composition of the Bacteroides microbiota and/or the MGE mobilities at the luminal vs. mucosal sites of the intestine.
Binaural Hearing in Bilateral Cochlear Implant Users: Electrophysiological and Clinical Correlation
Authors: Takeuti Alice Andrade , Andrade Joanlise Marco de Leon , de Carvalho Hugo Amilton Santos , Elias Thaís Gomes Abrahão , Takeuti Letícia Akemi Taniguchi , Caldas Fernanda Ferreira , Bahmad Fayez 
Published: August 2023

Abstract
Background
The main indication for bilateral cochlear implants (CIs) is the range of binaurality, a skill that allows sound localization and better auditory perception and discrimination in noisy environments. The presence of binaural hearing can be demonstrated electrophysiologically by the binaural interaction component (BIC) and clinically by the Hearing in Noise Test (HINT).
Objective
To analyze the correlation between latencies and amplitudes of the BIC of the cortical auditory evoked potential (BIC-CAEP) and HINT performance in bilateral CI users.
Methods
Pilot study consisted of 27 individuals with bilateral Cis in cochlear implant group (CIG), and the control group (CG) consisted of 22 volunteers with normal hearing. The latencies and amplitudes of the P1 and N1 components of the BIC-CAEP, auditory effort by a visual analog scale (VAS) and performance in the fixed and adaptive HINT were investigated. The results were compared between groups.
Results
There was a statistically significant difference between the CIG and CG in terms of VAS score of fixed HINT, performance in adaptive HINT, N1 and P1 amplitude of the BIC-CAEP. There was a significant correlation between the fixed HINT performance and the P1 amplitude of the BIC-CAEP.
Conclusion
Results of the present study demonstrated that lower P1 amplitude of the BIC-CAEP was correlated with best clinical performance in the fixed HINT.
A novel urease gene structure of Sporosarcina pasteurii with double operons
Authors: Pei Di , Liu Zhiming , Hu Biru 
Published: August 2023

Abstract
Sporosarcina pasteurii(S. pasteurii) is a soil-derived Gram-positive bacterium that has been used to develop a variety of application scenarios using its efficient urease activity to induce calcium carbonate production. However, the problems of uncontrollable and unstable mineralization reactions still existed in the current mineralization application studies of S. pasteurii, which were limited by the lack of basic theoretical studies, especially lacking the research on the structure and expression regulation mechanism of urease genes which play a central role in mineralization. The further discovery of the urease gene information of S. pasteurii urease will not only help to analyze the regulation mechanism of urease expression, but also promote the application of S. pasteurii mineralization materials. Based on the above situation, this research first compared the growth and urease gene expression of S. pasteurii under three different culture conditions by transcriptome sequencing, and then predicted the urease gene structure of S. pasteurii by bioinformatics method, and then verified the prediction results by molecular biology method. Finally, it was found that the urease gene of S. pasteurii is a double operons structure. This paper reveals for the first time the double operons structure of S. pasteurii urease, which greatly advances the mechanistic study of S. pasteurii-induced mineralization and is a crucial step forward in the regulation of urease expression. The discovery of the double operons structure is also of great significance for genetic engineering studies of urease.
Improving the Quality properties of soybean oil by using rice bran oil
Authors: mohamed Ahmed Sabry , Hashem Hanafy Abdel aziz , Abdel-Maksoud Badr Saed 
Published: August 2023

Abstract
This study aims to study effect of replacing soybean oil (SO) by rice bran oil (RBO) at levels (25,50 and75%) on physical, chemical properties, fatty acid composition and oxidative stability of SO and study the effect of storage at ambient temperature for 12 month on Free fatty acids content (% FFA), Peroxide value (PV) and Thiobarbituric acid (TBA) of SO, RBO and their blends oils. The RBO was of a good quality, as evidenced by its low initial values at % FFA, PV and TBA and has been recognized as an excellent source of γ oryzanol, it had the γ oryzanol meanwhile the other oils don’t contain γ oryzanol, Thus, the SO containing a higher amount of RBO leads to the least degradation, while pure SO has the highest, also had more inhibitory effect against oxidation due to a prolongation of the storage period without use of industrial antioxidants. FFA% and PV of all tested blends oils samples during the whole storage periods were found within the limits recommended for human consumption. TBA went in a parallel trend with those of PV. On the other hand, a gradual rise in TBA values was observed with extending the storage period of oils. In SO, the TBA increased from 0.533 mg malonaldehyde /kg oil at zero time to 1.446 mg alonaldehyde kg− 1 after 12th month of storage. While it increased from 0.336 mg malonaldehyde kg− 1 oil at zero time to 0.882 mg malonaldehyde kg− 1 oil after 12th month of storage in RBO.
Identification of metabolites reproducibly associated with Parkinson’s Disease via meta-analysis and computational modelling
Authors: Fleming Ronan , Luo Xi , Liu Yanjun , Balck Alexander , Klein Christine 
Published: August 2023

Abstract
Many studies have reported metabolomic analysis of different bio-specimens from Parkinson’s disease patients. However, inconsistencies in reported metabolite concentration changes make it difficult to draw conclusions as to the role of metabolism in the occurrence or development of Parkinson’s disease. We reviewed the literature on metabolomic analysis of Parkinson’s disease patients. From 74 studies that passed quality control metrics, perturbations to 928 metabolites were reported to be associated with PD diagnosis, but only 190 were replicated with the same changing trends in more than one study. Of these metabolites, 60 exclusively increased, such as 3-methoxytyrosine and glycine, 54 exclusively decreased, such as pantothenic acid and caffeine, and 76 inconsistently changed in concentration in PD versus control subjects, such as ornithine and tyrosine. A novel genome-scale metabolic model of PD and corresponding metabolic map linking most of the replicated metabolites enabled a better understanding of the dysfunctional pathways of PD and prediction of additional potential metabolic markers from pathways with consistent metabolite changes to target in future studies.
Analyzing the Structural Behaviour of Conducting Polymer Actuators and its Interdependence with the Electrochemical Phenomenon
Authors: Kumar Sukesh , Yu Aimin , Khandelwal Mudrika 
Published: August 2023

Abstract
This work reports the deformation behaviour of a conducting polymer poly(3,4-ethylene dioxythiophene):polystyrene sulfonate (PEDOT:PSS)/bacterial cellulose (BC) bi-layered actuator. It was found that the type and magnitude of the movement of actuator was strongly influenced by its width and length. Increasing the actuator’s cross-sectional area (width) would result in an increase in torsional deformation. Further, the maximum tip displacement trivially increased with length, but the degree of bending (curvature) decreased. Because the dimensions of actuator affect its deformation in a non-trivial manner, this can be advantageous from the design perspective in realizing different types of motions without incorporating additional materials.
Structural theory and electrochemical impedance Spectroscopy were further used to understand the mechanism of deformations depended on the dimension of a conducting polymer actuator. From the electrochemical impedance spectroscopy results it was understood that the higher bending in actuators of smaller lengths is likely caused by the higher extent of ion penetration within the PEDOT:PSS layer. The electric circuit modeling of the electrochemical impedance data revels the interdependence of the structural behaviour and the electrochemical phenomenon in a conducting polymer actuator.