bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety Profile of Intravitreal Injections in the Injection Cabinet: No Cases of Endophthalmitis Seen After 7,238 Injections
Authors: Ozgur Gokhan , Gokmen Onur 
Published: January 2023

Abstract
Aim: The present study evaluates the rate of endophthalmitis in intra vitreal injections (IVI) performed in a modular injection cabin that could be easily sited in a closet in an office environment, and the performance tests of the cabin in terms of heating, ventilation and air conditioning (HVAC).
Method: The injections given over approximately 18 months in the cabin and the demographics of the patients were screened retrospectively, and the cabin was subjected to HVAC tests.
Results: A total of 7,238 injections were performed in the defined period, and no cases of endophthalmitis were encountered (0%). The results of the air flow and flow rate, particle count, pressure difference, decontamination time, light, moisture and heat tests were all found to be above the standard defined by the International Organization for Standardization (ISO).
Conclusion: An injection cabin is a practical modular construction that can be easily sited in any space without the need for special climatization, and provides a sterile and safe environment for IVI.
A novel approach for the analysis of single-cell RNA sequencing identifies TMEM14B as a novel poor prognostic marker in hepatocellular carcinoma
Authors: Ma Ding , Liu Shuwen , He Qinyu , Kong Lingkai , Liu Kua , Xiao Lingjun , Xin Qilei , Bi Yanyu , Wu Junhua , Jiang chunping 
Published: January 2023

Abstract
Background
A fundamental goal in cancer-associated genome sequencing is to identify the key genes. Protein-protein interactions (PPIs) play a crucially important role in this goal. Here, human reference interactome (HuRI) map was generated and 64006 PPIs involving 9094 proteins were identified.
Methods
Here, we developed a physical link and co-expression combinatory network construction (PLACE) method for genes of interest, which provides a rapid way to analyze genome sequencingdatasets.Next, Kaplan‒Meier survival analysis, CCK8 assays,scratch wound assays and Transwell assays were applied to confirm the results.
Results
In this study, we selected single-cell sequencing data from patients with hepatocellular carcinoma (HCC) in GSE149614. The PLACE method constructs a protein connection network for genes of interest, and a large fraction (80%) of the genes (screened by the PLACE method) were associated with survival.Then,PLACE discovered that transmembrane protein 14B (TMEM14B) was the most significant prognostic key gene, and target genes of TMEM14B were predicted. The TMEM14B-target generegulatory network was constructed by PLACE. We also detected that TMEM14B-knockdown inhibited proliferation and migration.
Conclusions
The results demonstrate that we proposed a new effective method for identifying key genes. The PLACE method can be used widely and make outstanding contributions to the tumor research field.
Formal Analysis of Reconfigurable and Autonomous Robotic Arm]{Formal Analysis of Reconfigurable and Autonomous Robotic Arm
Authors: Rahim Muhammad Abdul Basit Ur , Raheem Muhammad Ahsan Ur , Sohail Muhammad Khalid , Farid Mohammad Atif , Mufti Muhammad Rafiq 
Published: January 2023

Abstract
Automation is one of the foremost technological trends in mining. Automation empowers mining companies to work around the clock and maximize productivity. Robotics brings a new degree of security to mines, from mechanical drills to self-driving ore trucks. The safety of these robots is also essential to ensure they should not damage themselves.
In this paper, we have formally modeled a robotic mining arm mechatronic system that could damage itself if a sensor were to fail. This report demonstrates how the damage caused by sensor failure can be prevented by formally modeling the system and verifiable properties to test that it would not damage itself during a sensor failure. The models and properties developed showed that the system would fail safely in such a scenario. Moreover, it is reconfigurable to deal with hazardous situations. We use the model-checking tool UPPAAL to model the robotic arm and verify it for safety, liveness, and deadlock-freeness properties.
Metagenomic analysis of oral plaques and aortic valve tissues reveals oral bacteria associated with aortic stenosis
Authors: Yoshiba Sayaka , Nakagawa Hirofumi , Kuwata Hirotaka , Nabuchi Akihiro , Yaso Atsutoshi , Shirota Tatsuo 
Published: January 2023

Abstract
Objectives
Endotoxins derived from bacteria in the oral cavity enter the bloodstream, leading to the onset of various systemic diseases, including heart valve disease. However, information on the oral bacteria involved in aortic stenosis is limited.
Materials and Methods
We comprehensively analyzed the microbiota in aortic valve tissues collected from patients with aortic stenosis using metagenomic sequencing and investigated the relationships between the valve microbiota and the oral microbiota and oral cavity conditions.
Results
Metagenomic analysis revealed the presence of 629 bacterial species in five oral plaque and 15 aortic valve clinical specimens. Patients were classified into two groups according to their aortic valve microbiota composition using principal coordinate analysis. Examination of the oral conditions of the patients showed no difference in the decayed/missing/filled teeth index; however, patients were divided into two groups (A and B) based on the aortic valve microbiota composition. Bacteria in group B tended to be associated with severe disease, and the number of bacteria on the dorsum of the tongue and the positive rate of bleeding during probing were significantly higher in this group than in group A. The pathophysiology of aortic stenosis may be related to the presence of oral bacteria such as Streptococcus oralis and Streptococcus sanguinis following bacteremia.
Conclusions
Systemic inflammation in severe periodontitis may be driven by the oral microbiota, supporting the indirect (inflammatory) association between oral bacteria and aortic stenosis.
Clinical Relevance:
Appropriate oral hygiene management may contribute to the prevention and treatment of aortic stenosis.
A functional electrochemical sensing platform for the detection of lead ions
Authors: Khaleel Mustafa , Soylu Pervin Deveci 
Published: January 2023

Abstract
A novel electrochemical sensor for the detection of lead ions was constructed by using electrodeposition of gold nanoparticles (GNPs) and glutathione (GSH) onto reduced graphene oxide (rGO) to form a GSH@GNP@rGO nanocomposite on a glassy carbon electrode (GSH@GNP@rGO@GCE). The sensing properties of the obtained sensor were tested by Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). Under optimized experimental conditions, the voltammetry response gradually raises by increasing the concentration (2-20 μΜ). The result showed that the GSH@GNP@rGO@GCE sensor exhibited high sensitivity towards Pb(II) with a low detection limit of 0.43 μMby DPV. Our studies suggest that the GSH@GNP@rGO nanocomposite could potentially be used for detecting Pb(II).
Preoperative magnetic resonance imaging of anal fistulas with scrotal extension
Authors: Vo Duc , Nguyen Nam , Nguyen Linh , Nguyen Thien , Phan Chien , Nguyen Truc 
Published: January 2023

Abstract
The aim of this study was to elucidate the magnetic resonance (MR) characteristics of anal fistulas extending to the scrotum, the rules applicable to them, and to correlate MR features with surgical findings. We conducted a retrospective study in 150 consecutive patients with anal fistulas extending into the scrotum, who were diagnosed and underwent surgery at University Medical Center Ho Chi Minh City between January 2017 and April 2022. MR findings were evaluated and compared with surgical findings using the Cohen’s kappa coefficient (k) with a 95% confidence interval. 150 patients (mean age 37.6 ± 10.9 years) with 166 fistulas, including 150 anal fistulas with scrotal extension. Most fistulas were low transsphincteric (80.0%, 120/150 patients). There was strong agreement for primary tract classification and detecting the location of internal openings between MRI and surgical findings with k = 0.83 (0.78–0.87) and k = 0.89 (0.85 – 0.93) (p<0.001), respectively. There is a significant correlation between the location of internal openings and the type of fistula (p<0.05). Low transsphincteric fistulas were predominant in the anterior group (103/122 patients vs. 10/19 patients), while in the posterior group it was more common in the high transsphincteric fistulas (7/19 patients vs. 14/122 patients), and the intersphincteric fistulas (1/19 patients vs. 5/122 patients); and the suprasphincteric fistulas were only seen in posterior group (1 patient). Anal fistulas with scrotal extension are exceptions to Goodsall’s rule. Albeit being long-tract fistulas, most of them are low transsphincteric and have anterior internal openings.
Evolvable Design of Network-oriented Services based on Core/Periphery Structure
Authors: Takagi Shiori , Arakawa Shin'ichi , Murata Masayuki 
Published: January 2023

Abstract
In the recent years, many new network-oriented services have emerged, and such services will need to be virtualized in the multi-access edge computing (MEC) environment, which is currently being standardized along with fifth generation (5G). The service design should be adaptable to user requirements and environmental changes for accommodating a large number of services at low cost. In addition, it is required not only to assume environmental changes when initially designing the service functions network, but also to enable the network to continue to change its structure to adapt to new environmental changes in the future. We have been investigated a core/periphery structure that allows service to effectively adapt to each user request and environmental variation. The advantage of the core/periphery structure is that it helps reduce the costs for maintaining or changing services by distinguishing the service functions into core and periphery functions. In this paper, we propose a method to evolve service functions network based on a core/periphery structure. Adapting to environmental changes requires the addition of interfaces between service functions or the development of new service functions. Our method evolves the structure of the service function network at low cost by keeping the core and peripheral functions at the appropriate scale. Our simulation results reveal that the structure of the service function networks can continue to evolve for a low cost and can maintain a high service accommodation ratio.
Social Determinants of Health: A Need for Evidence-Based Guidelines on How to Capture Data on Underserved Patients
Authors: Tran Nguyen H. , Almodallal Yahya , Batheja Mashal , Martin Nicole , Le-Rademacher Jennifer , Ridgeway Jennifer , Sia Irene G. , Jatoi Aminah 
Published: January 2023

Abstract
Background. Social determinants of health lead to better cancer care. This multi-site, single-institution study sought to capture data on social determinants of health data in Asian Americans with hepatocellular carcinoma; this group constitutes 60% of patients with this malignancy and are often undertreated or not treated at all.
Methods. This study took advantage of an institutional initiative designed to capture and integrate social determinants of health data into the electronic medical record for all patients. Medical records of Asian Americans with hepatocellular cancer were reviewed to acquire data on housing instability, lack of transportation, financial concerns, and social isolation; a score of 1 indicated poor social determinants of health.
Results. Of 112 adult Asian American patients with hepatocellular cancer, 22 (20%) were Southeast Asian, and 74 (67%) described English proficiency. A score of 1 (highest risk) was observed in 1 patient (0.9%) for housing instability; 1 (0.9%) lack of transportation; no patient for financial hardship; and 1 (0.9%) for social isolation. However – and importantly -- total noncompletion per domain (no question answered within that domain) was observed in 90 patients (80%) for housing instability; 90 (80%) for lack of transportation; 92 (82%) for financial hardship; and 90 (80%) for social isolation. Of note, institution-wide benchmark total noncompletion rates were 0.3%, 0.3%, 47%, and 39% for these respective domains.
Conclusion. High total noncompletion rates make social determinants of health data challenging to interpret and underscore the need for evidence-based guidelines on how best to capture such data in underserved patients.
Open water dreissenid mussel control projects: lessons learned from a retrospective analysis
Authors: Dahlberg Angelique D , Waller Diane L , Hammond David , Lund Keegan , Phelps Nicholas BD 
Published: January 2023

Abstract
Dreissenid mussels are one of the most problematic aquatic invasive species (AIS) in North America, causing significant ecological and economic impacts. To date, dreissenid mussel control efforts in open water have included physical, biological, and chemical methods. The feasibility of successful dreissenid mussel management or eradication in lakes is relatively undocumented in the freshwater management literature. This review presents information on 33 open water dreissenid mussel control projects in 23 North America lakes. We reviewed data from past dreissenid mussel control projects and identified patterns and knowledge gaps to help inform adaptive management strategies. The three key lessons learned include 1) pre- and post-treatment survey methods should be designed to meet management objectives, e.g., by sampling for all life stages and taking into account that no survey method is completely comprehensive; 2) defining the treatment area – particularly ensuring it is sufficiently large to capture all life stages present – is critical to meeting management objectives; and 3) control projects provide an opportunity to collect mortality, depth, water chemistry, eDNA, effects on non-target organisms, and other efficacy-related data that can inform safe and effective adaptive management.
Reactivity properties of the HOSO and HSO2 isomers in liquid medium: A sequential Monte-Carlo/Quantum-Mechanics Study
Authors: Junqueira Geórgia M.A. , Ballester Maikel Y. , Nascimento Marcos A. C. 
Published: January 2023

Abstract
Understanding the atmospheric sulfur cycle is fundamental in developing actions to mitigate its effects on acid rain formation. Solvation of HOSO and HSO2 species has been indicated as a possible pathway in sulfur acid formation. To observe differences in the behavior of these isomers in the water medium, electrical and reactivity properties - electronic chemical potential (μ), chemical hardness (η), and electrophilicity (ϵ)- have been obtained. Once the respective first solvation shells (15H2O for HSO2 and 23 H2O for HOSO) are considered, an increase of 25% in the dipole moment of HSO2 was observed, while the dipole moment of HOSO decreases in 11%. Both solvated isomers become softer (η decreases) compared to gas phase.
Methods: HOSO and HSO2 radicals in the presence of water are investigated through a sequential Monte Carlo/ Quantum Mechanics methodology. There, the intermolecular interactions are described by the Lennard-Jones plus the Coulomb potentials. For this, the DICE program was employed. The quantum calculations were performed at CASPT2/aug−cc− pV(T+d)Z level of theory through MOLPRO computational package.
Identification of key genes related to immune cells in patients with COVID-19 via Integrated Bioinformatics-Based Analysis
Authors: Chen Zhao-jun , Chen Hai-hua 
Published: January 2023

Abstract
Background
COVID-19 has spread all over the world which poses a serious threat to social economic development and public health. Despite enormous progress has been made in the prevention and treatment of COVID-19, the specific mechanism and biomarker related to disease severity or prognosis have not been clarified yet. Our study intended to further explore the diagnostic markers of COVID-19 and their relationship with serum immunology by bioinformatics analysis.
Methods
The datasets about COVID-19 were downloaded from the Gene Expression Omnibus (GEO) dataset. The differentially expressed genes (DEGs) were selected via the limma package. Then, weighted gene co-expression network analysis (WGCNA) was conducted to identify the critical module associated with the clinic status. The intersection DEGs were processed for further enrichment analysis. The final diagnostic genes for COVID-19 were selected and verified through special bioinformatics algorithms.
Results
There were significant DEGs between the normal and COVID-19 patients. These genes were mainly enriched in cell cycle, complement and coagulation cascade, extracellular matrix (ECM) receptor interaction, and the P53 signalling pathway. As much as 358 common intersected DEGs were selected in the end. These DEGs were enriched in organelle fission, mitotic cell cycle phase transition, DNA helicase activity, cell cycle, cellular senescence, and P53 signalling pathway. Our study also identified CDC25A, PDCD6, and YWAHE were potential diagnostic markers of COVID-19 with the AUC (area under curve), 0.958 (95% CI: 0.920–0.988), 0.941(95% CI: 0.892 − 0.980), and 0.929(95% CI: 0.880 − 0.971). Moreover, CDC25A, PDCD6, and YWAHE were correlated with plasma cells, macrophages M0, T cells CD4 memory resting, T cells CD8, dendritic cells, and NK cells.
Conclusions
Our study discovered that CDC25A, PDCD6 and YWAHE can be used as diagnostic markers for COVID-19. Moreover, these biomarkers were also closely associated with immune cell infiltration, which plays a pivotal role in the diagnosis and progression of COVID-19.
Rare gene mutations of wild-type gastrointestinal stromal tumors
Authors: Liu Qiuyu , Li Chunxiao , Li Zhen , Fu Fangfang , Han Qian , Zhu Haohui , Bai Xiaoyun , Gao Xuejuan , Kong Lingfei 
Published: January 2023

Abstract
Background
Wild-type (WT) gastrointestinal stromal tumors (GISTs) are a subgroup of GISTs lacking KIT and PDGFRA mutations. Further gene and clinical characterization of WT GISTs will help determining patient prognosis and developing targeted therapy.
Methods
A total of 105 patients with GISTs were enrolled, and 15 were WT GISTs. Immunohistochemistry (IHC) staining, Sanger sequencing (SS) and next generation sequencing (NGS) approach were used to analyze WT GISTs.
Results
The 15 WT GISTs (median age, 50 [range, 9–82] years; 66.6% female; 73.3% stomach) showed the following gene mutations. SDH-deficient WT GISTs (n = 7) had mutations in SDHA, CCND1, RB1, FLT3, TP53 and SDHD. Patient with SDHA (c.G1690A), CCND1, RB1, and FLT3 (c.C2917T) mutations was a middle-aged women, stomach tumor location and high proliferative activity. Patients with TP53 ( c.300_308delGAAAACCTA; c.G841T) mutation or coexisting mutation of SDHD (c.336de1T) were showed high p53 expression, middle-aged women, stomach tumor location. Non-SDH-deficient WT GISTs (n = 8) had mutations in BRAF, TERT, and CDH1. Two patients harbored BRAF (V600E) mutation: one was a micro-GISTs, elderly female, stomach location, and low risk, the other was accompany TERT mutation, elderly male, small intestine location, and high risk. Patient with CDH1 (c.G2356A) mutation was a middle-aged male, duodenum tumor location and high risk.
Conclusions
We identified rare gene mutations of CCND1, RB1, FLT3, TERT, and CDH1 in WT GISTs. what's more, mutations of CCND1, RB1, TP53, BRAF, and CDH1 were likely to show high risk and malignant behavior which will provided new insights into the biology and mutations in WT GISTs.
Knowledge of, and factors associated with current use of modern contraceptives among young people 10 - 24years in central and western Uganda: A cross-sectional study
Authors: Bunani Nelson , Tweheyo Raymond , Beavaline Evelyne Nyachwo , Kabagenyi Allen , Neema Stella , Rutebemberwa Elizeus 
Published: January 2023

Abstract
Background
Although modern contraceptives are the most effective way for averting unintended pregnancies and related adverse reproductive events for the mother and baby, their use in Uganda remains low compared to the national target for the year 2020. Adolescents and young people start using modern contraceptives at 23.8 years, yet most of them have had sexual intercourse by age 16. The objective of the study was to determine the knowledge of, and factors associated with the current use of modern contraceptives among young people 10–24 years in central and western Uganda.
Methods
This was a cross cross-sectional study. Data was collected from 289 in- and out-of-school young people aged 10–24 years in five districts of western and central Uganda between July and August 2020. Digital questionnaires designed in Census and Survey Processing System (CSPro) version 7.5.1 were used. Data was transferred to Stata 15.1 for analysis. Modified Poisson regression at bivariable and multivariable analysis was used to determine the factors associated with modern contraceptive use.
Results
About 62.6% of the respondents had high knowledge of modern contraceptives. Most respondents were highly knowledgeable on injectable contraceptives 278 (96.2%) and male condoms (96.5%). Emergency contraceptives were known by only 138 (47.8%) of the respondents. In addition, the current use of modern contraceptives was 58.5% (169). Modern contraceptive use was significantly associated with being aged 20–24 years (Adj. PRR = 0.69, 95%CI; 0.52–0.90), p = 0.007; not residing with someone as a sexual partner (Adj. PRR = 0.71, 95%CI; 0.57–0.88), p = 0.002; and being a student (Adj. PRR = 1.37, 95% CI; 1.04–1.79), p = 0.023.
Conclusion
Young people 10–24 years in central and western Uganda were highly knowledgeable about modern contraceptives, particularly injectables, and condoms, but least knowledgeable about emergency contraceptives. Respondents who were students compared to non-students were more likely to be using modern contraceptives, while those aged 20–24 years and those not staying with their sexual partners were less likely to use modern contraceptives. Awareness campaigns among young people aged 20–24 years and those co-residing with a sexual partner will be necessary to improve modern contraceptive use among young people aged 10–24 years.
Growth and adaptability of provenances and progenies of Pinus maximinoi h. E. Moore in northern Mozambique
Authors: Dias Cremildo Riba Gouveia , Guacha Laurina Adriano , Mbanze Aires Afonso 
Published: January 2023

AbstractThe aim of this study was to evaluate the growth and adaptability of Pinus maximinoi provenances and progenies and to estimate the genetic variation that could be exploited in future breeding work. The company Floresta de Niassa Lda established a trial with four provenances and nineteen progenies in the Chimbonila district of northern Mozambique. The field trial was set up in a randomized block design with four repetitions and six-plant linear plots. At two, four and eight years of age, performance in total height, diameter at breast height (DBH) and survival were analysed. The results at eight years of age showed no significant variation between provenances for all variables analysed, and any of these provenances can be used for reforestation in northern Mozambique. The first group was composed of 9 (45%) of the best performing progenies, mostly from Yuscaran and San Jeronimo, and the second (last) group was composed of 11 (55%) of the worst performing progenies, mostly from Tatumbla and Coban. Most of the nine best classified progenies based on the predicted additive genetic effect and genetic gain belonged to Yuscaran and Tatumbla. On the other hand, most of the progenies at the level of the seventeen best classified individuals belonged to San Jeronimo and can be used for future breeding projects in northern Mozambique.