bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations of food motives with red meat and legume consumption in the population-based DILGOM study
Authors: Hentilä Annukka , Männistö Satu , Kaartinen Niina E. , Jousilahti Pekka , Konttinen Hanna 
Published: August 2023

Abstract
Purpose
To improve human health and environmental sustainability, red meat consumption should decrease and legume consumption increase in diets. More information on food motives, however, is required when developing more tailored and effective interventions targeting legume and meat consumption. We aimed to examine the associations between food motives and red meat and legume consumption, and whether these associations differ between different subgroups (gender, age groups, marital status, education, BMI).

Methods
Ten food motives (health, mood, convenience, sensory appeal, natural content, price-cheap, price-value, weight control, familiarity and ethical concern measured with Food Choice Questionnaire) were studied in 3079 Finnish adults in the population-based DILGOM study. Food consumption was assessed with Food Frequency Questionnaire. The adjusted estimates from multivariable regression models are reported.

Results
Higher relative importance of natural content (β = − 0.275, 95% CI − 0.388; − 0.162) and ethical concern (β = − 0.462, 95% CI − 0.620; − 0.305) were associated with lower red meat consumption, and higher appreciation of sensory appeal (β = 0.482, 95% CI 0.347; 0.616) and price-cheap (β = 0.190, 95% CI 0.099; 0.281) with higher red meat consumption. Higher importance of health (β = 0.608, 95% CI 0.390; 0.825) was associated with higher legume consumption, and higher appreciation of convenience (β = − 0.401, 95% CI − 0.522; − 0.279), price-value (β = − 0.257, 95% CI − 0.380; − 0.133) and familiarity (β = − 0.278, 95% CI − 0.393; − 0.164) with lower legume consumption. The associations of particularly ethical concern, weight control, sensory appeal and mood varied according to gender, age, marital status or BMI.

Conclusion
The development and implementation of actions to decrease red meat and increase legume consumption should focus on several food motives across different subgroups.
Comparison of cementless twin-peg, cemented twin-peg and cemented single-peg femoral component migration after medial unicompartmental knee replacement: a 5-year randomized RSA study
Authors: Mosegaard Sebastian Breddam , Odgaard Anders , Madsen Frank , Rømer Lone , Kristensen Per Wagner , Vind Tobias Dahl , Søballe Kjeld , Stilling Maiken 
Published: August 2023

Abstract
Background
The component design and fixation method of joint arthroplasty may affect component migration and survival. The aim of this study was to compare fixation of cementless twin-peg (CLTP), cemented twin-peg (CTP) and cemented single-peg (CSP) femoral components of medial unicompartmental knee replacement (UKR).

Methods
Eighty patients (mean age = 63 years, 48 males) with medial knee osteoarthritis were randomized in three ways to CLTP (n = 25), CTP (n = 26) or CSP (n = 29) femoral UKR components. The patients were followed 5 years postoperatively with RSA, bone mineral density (BMD), PROMs and radiological evaluation of radiolucent lines (RLL), femoral component flexion angle and complications.

Results
At the 5-year follow-up, femoral component total translation was comparable between the three groups (p = 0.60). Femoral component internal rotation was 0.50° (95% CI 0.3; 0.69) for the CLTP group, 0.58° (95% CI 0.38; 0.77) for the CTP group and 0.25° (95% CI 0.07; 0.43) for the CSP group (p = 0.01). BMD decreased peri-prosthetically (range − 11.5%; − 14.0%) until 6-month follow-up and increased toward the 5-year follow-up (range − 3.6%; − 5.8%). BMD change did not correlate with component migration. Lower flexion angle was correlated with higher 5-year subsidence, total translation, varus rotation and maximum total point motion (p = 0.01). Two patients (1 CLTP, 1 CTP) had RLL in the posterior zone. There were two revisions.

Conclusion
At 5-year follow-up, fixation of UKA femoral components with twin-peg was not superior to the single-peg design. Cementless and cemented twin-peg femoral components had similar fixation. A lower flexion angle was correlated with higher component migration.
Evolution of macular atrophy in eyes with neovascular age-related macular degeneration compared to fellow non-neovascular eyes
Authors: Blazaki Styliani , Blavakis Emmanouil , Chlouverakis Gregory , Bontzos Georgios , Chatziralli Irini , Smoustopoulos Georgios , Dimitriou Eleni , Stavrakakis Anastasios , Kabanarou Stamatina , Xirou Tina , Vavvas Demetrios G. , Tsilimbaris Miltiadis K. 
Published: August 2023

Abstract
Purpose
Τo evaluate the evolution of macular atrophy (MA) in patients with neovascular AMD (nAMD), compared with their fellow eyes exhibiting dry AMD (dAMD).

Methods
This retrospective study included 124 patients from three centers treated with anti-VEGF in their nAMD eye and having dAMD in the fellow eye. Patients without MA at baseline were analyzed to study the time to first MA development. Synchronous and unsynchronous time course of MA was also studied. MA was evaluated using near-infrared images, while all available optical coherence tomography (OCT) images were used to confirm the criteria proposed by the Classification of Atrophy Meetings group for complete MA.

Results
MA first detection in nAMD eyes increased significantly from year 2 to 6 compared to dAMD eyes. Over the study’s follow-up, 45.1% of nAMD-E developed MA, compared to 16.5% of fellow eyes (p < 0.001). When MA in the two eyes was compared in a synchronous paired manner over 4 years, nAMD eyes had an average MA progression rate of 0.275 mm/year versus 0.110 mm/year in their fellow dAMD eyes. Multivariate ANOVA revealed significant time (p < 0.001), eye (p = 0.003), and time-eye interaction (p < 0.001) effects. However, when MA did develop in dAMD eyes and was compared in an asynchronous manner to MA of nAMD eyes, it was found to progress faster in dAMD eyes (dAMD: 0.295 mm/year vs. nAMD: 0.176 mm/year) with a significant time-eye interaction (p = 0.015).

Conclusions
In this study, a significant difference in MA incidence and progression was documented in eyes with nAMD under treatment, compared to fellow eye exhibiting dAMD. Eyes with nAMD tended to develop more MA compared to fellow dAMD eyes. However, when atrophy did develop in the fellow dAMD eyes, it progressed faster over time compared to MA in nAMD eyes.
The late-evolving salmon and trout join the GnRH1 club
Authors: von Schalburg Kristian R. , Gowen Brent E. , Christensen Kris A. , Ignatz Eric H. , Hall Jennifer R. , Rise Matthew L. 
Published: August 2023

AbstractAlthough it is known that the whitefish, an ancient salmonid, expresses three distinct gonadotropin-releasing hormone (GnRH) forms in the brain, it has been thought that the later-evolving salmonids (salmon and trout) had only two types of GnRH: GnRH2 and GnRH3. We now provide evidence for the expression of GnRH1 in the gonads of Atlantic salmon by rapid amplification of cDNA ends, real-time quantitative PCR and immunohistochemistry. We examined six different salmonid genomes and found that each assembly has one gene that likely encodes a viable GnRH1 prepropeptide. In contrast to both functional GnRH2 and GnRH3 paralogs, the GnRH1 homeolog can no longer express the hormone. Furthermore, the viable salmonid GnRH1 mRNA is composed of only three exons, rather than the four exons that build the GnRH2 and GnRH3 mRNAs. Transcribed gnrh1 is broadly expressed (in 17/18 tissues examined), with relative abundance highest in the ovaries. Expression of the gnrh2 and gnrh3 mRNAs is more restricted, primarily to the brain, and not in the gonads. The GnRH1 proximal promoter presents composite binding elements that predict interactions with complexes that contain diverse cell fate and differentiation transcription factors. We provide immunological evidence for GnRH1 peptide in the nucleus of 1-year-old type A spermatogonia and cortical alveoli oocytes. GnRH1 peptide was not detected during other germ cell or reproductive stages. GnRH1 activity in the salmonid gonad may occur only during early stages of development and play a key role in a regulatory network that controls mitotic and/or meiotic processes within the germ cell.
The context-dependent role of the Na+/Ca2+-exchanger (NCX) in pancreatic stellate cell migration
Authors: Loeck Thorsten , Rugi Micol , Todesca Luca Matteo , Kalinowska Paulina , Soret Benjamin , Neumann Ilka , Schimmelpfennig Sandra , Najder Karolina , Pethő Zoltán , Farfariello Valerio , Prevarskaya Natalia , Schwab Albrecht 
Published: August 2023

AbstractPancreatic stellate cells (PSCs) that can co-metastasize with cancer cells shape the tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) by producing an excessive amount of extracellular matrix. This leads to a TME characterized by increased tissue pressure, hypoxia, and acidity. Moreover, cells within the tumor secrete growth factors. The stimuli of the TME trigger Ca2+ signaling and cellular Na+ loading. The Na+/Ca2+ exchanger (NCX) connects the cellular Ca2+ and Na+ homeostasis. The NCX is an electrogenic transporter, which shuffles 1 Ca2+ against 3 Na+ ions over the plasma membrane in a forward or reverse mode. Here, we studied how the impact of NCX activity on PSC migration is modulated by cues from the TME. NCX expression was revealed with qPCR and Western blot. [Ca2+]i, [Na+]i, and the cell membrane potential were determined with the fluorescent indicators Fura-2, Asante NaTRIUM Green-2, and DiBAC4(3), respectively. PSC migration was quantified with live-cell imaging. To mimic the TME, PSCs were exposed to hypoxia, pressure, acidic pH (pH 6.6), and PDGF. NCX-dependent signaling was determined with Western blot analyses. PSCs express NCX1.3 and NCX1.9. [Ca2+]i, [Na+]i, and the cell membrane potential are 94.4 nmol/l, 7.4 mmol/l, and − 39.8 mV, respectively. Thus, NCX1 usually operates in the forward (Ca2+ export) mode. NCX1 plays a differential role in translating cues from the TME into an altered migratory behavior. When NCX1 is operating in the forward mode, its inhibition accelerates PSC migration. Thus, NCX1-mediated extrusion of Ca2+ contributes to a slow mode of migration of PSCs.
A comprehensive analysis of Fanconi anemia genes in Chinese patients with high-risk hereditary breast cancer
Authors: Zhu Qiao-Yan , Li Pu-Chun , Zhu Yi-Fan , Pan Jia-Ni , Wang Rong , Li Xiao-Lin , Ye Wei-Wu , Ding Xiao-Wen , Wang Xiao-Jia , Cao Wen-Ming 
Published: August 2023

Abstract
Background
Four Fanconi anemia (FA) genes (BRCA1, BRCA2, PALB2 and RAD51C) are defined as breast cancer (BC) susceptibility genes. Other FA genes have been inconsistently associated with BC. Thus, the role of other FA genes in BC should be explored in specific populations.

Methods
Mutations in 16 FA genes were screened with a 98-gene panel sequencing assay in a cohort of 1481 Chinese patients with high-risk hereditary BC. The association between mutations and clinicopathological characteristics as well as prognosis was analyzed. The risk of BC in carriers of FA gene mutations was assessed in the Genome Aggregation Database and the Westlake Biobank for Chinese cohort.

Results
A total of 2.57% (38/1481) BC patients were identified who had 12 other FA gene germline mutations. Among them, the most frequently mutated gene was FANCA (8/1481, 0.54%). These 38 patients carried 35 distinct pathogenic/likely pathogenic variants, of which 21 were novel. We found one rare FANCB deleterious variant (c.1327-3dupT) in our cohort. There was a statistically significant difference in lymph node status between FA gene mutation carriers and non-carriers (p = 0.041). We observed a trend that mutation carriers had larger tumor sizes, lower estrogen receptor (ER) and progesterone receptor (PR) positivity rates, and lower 3.5-year invasive disease-free survival (iDFS) and distant recurrence-free survival (DRFS) rates than non-carriers (tumor size > 2 cm: 51.43% vs. 45.63%; ER positivity rates: 51.43% vs. 60.81%; PR positivity rates: 48.57% vs. 55.16%; 3.5-year iDFS rates: 58.8% vs. 66.7%; 3.5-year DRFS rates: 58.8% vs. 68.8%). The frequency of the mutations in FANCD2, FANCM and BRIP1 trended to be higher among BC cases than that in controls (p = 0.055, 0.08 and 0.08, respectively).

Conclusion
This study comprehensively estimated the prevalence, clinicopathological characteristics, prognosis and risk of BC associated with deleterious variants in FA genes in Chinese high-risk hereditary BC patients. It enriches our understanding of the role of FA genes with BC.
Wound reaction to trunk injection of oxytetracycline or water in huanglongbing-affected sweet orange (Citrus sinensis) trees
Authors: Archer Leigh , Albrecht Ute 
Published: August 2023

Abstract
Key message
Trunk injection causes injury to trees and best practices must be established for use of this technology to manage vascular diseases such as huanglongbing.

Abstract
Trunk injection is a technique for applying plant protection compounds that has demonstrated efficacy for management of huanglongbing (HLB), a bacterial disease which has devastated the Florida citrus industry. Recently, the antibacterial oxytetracycline has been approved for trunk injection in citrus in Florida. Despite its potential for restoring health of HLB-affected trees, little information is available on the trees’ ability to heal and compartmentalize the wounds caused by trunk injection and the interaction with season and other factors. In this study, 5-year-old HLB-affected sweet orange (Citrus sinensis) trees were injected bi-monthly to measure seasonal differences in the rate of uptake of injected water and to compare the external and internal wound reaction in both scion and rootstock. Also investigated was the wound reaction after trunk injection of an injectable formulation of oxytetracycline. Injection during spring and summer promoted more rapid wound closure compared to injection in fall or winter, and injection into the scion was less damaging than injection into the rootstock. Although citrus trees effectively compartmentalized wounds inflicted by injection of water, injection of oxytetracycline impeded wound healing and increased internal damage. Application of a fungicide or pruning sealant increased the size of the wound internally and externally. For trunk injection to be adopted for use in commercial citrus orchards, the benefits of injection must outweigh the risks of wounding associated with the application method. This study provides first insight on some of the best practices for effectively utilizing trunk injection in citrus as a crop protection strategy.
Efficacy of ceftazidime-avibactam, meropenem-vaborbactam, and imipenem-relebactam combinations against carbapenemase-producing Enterobacterales in Switzerland
Authors: Nordmann Patrice , Bouvier Maxime , Poirel Laurent 
Published: August 2023

AbstractCarbapenemase-producing in Enterobacterales (CPE) represent a critical health concern worldwide, including in Switzerland, leading to very limited therapeutic options. Therefore, our aim was to evaluate the susceptibility to the novel ß-lactam/ß-lactamase inhibitor combinations ceftazidime-avibactam, meropenem-vaborbactam, and imipenem-relebactam of CPE isolates recovered in Switzerland from 2018 to 2020. A total of 150 clinical CPE were studied including mainly Klebsiella pneumoniae (n = 61, 40.3%) and Escherichia coli (n = 53, 35.3%). The distribution of carbapenemases was as follows: KPC-like (32%), OXA-48-like (32%), NDM-like (24%), combinations of carbapenemases (10%), VIM-1 producers (n = 2), and a single IMI-1 producer. Overall, 77% of the strains were susceptible to meropenem-vaborbactam, 63% was susceptible to ceftazidime-avibactam, and 62% susceptible to imipenem-relebactam. Those data may contribute to optimize the choice of first line therapy for treating infections due to CPE.
A numerical study of the effect of thrombus breakdown on predicted thrombus formation and growth
Authors: Wang Kaihong , Armour Chlöe H. , Gibbs Richard G. J. , Xu Xiao Yun 
Published: August 2023

AbstractThrombosis is a complex biological process which involves many biochemical reactions and is influenced by blood flow. Various computational models have been developed to simulate natural thrombosis in diseases such as aortic dissection (AD), and device-induced thrombosis in blood-contacting biomedical devices. While most hemodynamics-based models consider the role of low shear stress in the initiation and growth of thrombus, they often ignore the effect of thrombus breakdown induced by elevated shear stress. In this study, a new shear stress-induced thrombus breakdown function is proposed and implemented in our previously published thrombosis model. The performance of the refined model is assessed by quantitative comparison with experimental data on thrombus formation in a backward-facing step geometry, and qualitative comparison with in vivo data obtained from an AD patient. Our results show that incorporating thrombus breakdown improves accuracy in predicted thrombus volume and captures the same pattern of thrombus evolution as measured experimentally and in vivo. In the backward-facing step geometry, thrombus breakdown impedes growth over the step and downstream, allowing a stable thrombus to be reached more quickly. Moreover, the predicted thrombus volume, height and length are in better agreement with the experimental measurements compared to the original model which does not consider thrombus breakdown. In the patient-specific AD, the refined model outperforms the original model in predicting the extent and location of thrombosis. In conclusion, the effect of thrombus breakdown is not negligible and should be included in computational models of thrombosis.
Diagnostic difficulties in pediatric annular dermatoses
Authors: Weiss Doris , Weber Philipp , Hampel Amélie , Tittes Julia , Weninger Wolfgang , Kinaciyan Tamar 
Published: August 2023

SummaryThe polymorphic presentation of annular dermatoses in the pediatric population renders them a diagnostic challenge to the clinician. They include various distinct disease entities that can be vaguely categorized according to the age of onset. Herein, we report on a young girl with clinical characteristics of Wells’ syndrome, while histological findings favored the diagnosis of annular erythema of infancy (AEI). Although morphological and histological similarities do exist, AEI and eosinophilic annular erythema (EAE) of childhood are considered as distinct entities in the literature. Wells’ syndrome (WS) is an eosinophilic dermatosis and histologically characterized by eosinophilic dermal infiltration with the hallmark feature of “flame figures.” Based on this case, we discuss and review the differential diagnoses of annular dermatoses in children.
Deep neural network-based clustering of deformation curves reveals novel disease features in PLN pathogenic variant carriers
Authors: Taha Karim , van de Leur Rutger R. , Vessies Melle , Mast Thomas P. , Cramer Maarten J. , Cauwenberghs Nicholas , Verstraelen Tom E. , de Brouwer Remco , Doevendans Pieter A. , Wilde Arthur , Asselbergs Folkert W. , van den Berg Maarten P. , D’hooge Jan , Kuznetsova Tatiana , Teske Arco J. , van Es René 
Published: August 2023

AbstractEchocardiographic deformation curves provide detailed information on myocardial function. Deep neural networks (DNNs) may enable automated detection of disease features in deformation curves, and improve the clinical assessment of these curves. We aimed to investigate whether an explainable DNN-based pipeline can be used to detect and visualize disease features in echocardiographic deformation curves of phospholamban (PLN) p.Arg14del variant carriers. A DNN was trained to discriminate PLN variant carriers (n = 278) from control subjects (n = 621) using raw deformation curves obtained by 2D-speckle tracking in the longitudinal axis. A visualization technique was used to identify the parts of these curves that were used by the DNN for classification. The PLN variant carriers were clustered according to the output of the visualization technique. The DNN showed excellent discriminatory performance (C-statistic 0.93 [95% CI 0.87–0.97]). We identified four clusters with PLN-associated disease features in the deformation curves. Two clusters showed previously described features: apical post-systolic shortening and reduced systolic strain. The two other clusters revealed novel features, both reflecting delayed relaxation. Additionally, a fifth cluster was identified containing variant carriers without disease features in the deformation curves, who were classified as controls by the DNN. This latter cluster had a very benign disease course regarding development of ventricular arrhythmias. Applying an explainable DNN-based pipeline to myocardial deformation curves enables automated detection and visualization of disease features. In PLN variant carriers, we discovered novel disease features which may improve individual risk stratification. Applying this approach to other diseases will further expand our knowledge on disease-specific deformation patterns.
Graphical abstract

Overview of the deep neural network-based pipeline for feature detection in myocardial deformation curves. Firstly, phospholamban (PLN) p.Arg14del variant carriers and controls were selected and a deep neural network (DNN) was trained to detect the PLN variant carriers. Subsequently, a clustering-based approach was performed on the attention maps of the DNN, which revealed 4 distinct phenotypes of PLN variant carriers with different prognoses. Moreover, a cluster without features and a benign prognosis was detected.
Dissolution of viscose rayon multifilament yarn in the ionic liquid 1-ethyl-3-methylimidazolium acetate studied using time–temperature superposition
Authors: Alanazi Maer , Ries Michael E. , Hine Peter J. 
Published: August 2023

AbstractWide-angle X-ray diffraction (WAXS) and mechanical testing techniques are used to track the dissolution of a viscose rayon multifilament yarn in the ionic liquid 1-ethyl-3-methyl-imidazolium acetate [C2mim]+ [OAc]− for different times and temperatures. In the dissolution process, the oriented cellulose II crystals in the regenerated cellulose fibres dissolve and then reform into randomly oriented crystals to form a matrix phase, and this change in orientation enables us to follow the dissolution process using WAXS, and hence determine the dissolved matrix volume fraction $${v}_{m}$$

v
m

. The change in the average molecular orientation $${P}_{2}$$

P
2

determined from an azimuthal $$(\alpha$$

(
α

) X-ray scan, allows the growth of the matrix volume fraction $${v}_{m}$$

v
m

to be calculated with time and temperature. The growth of $${v}_{m}$$

v
m

was found to follow time temperature superposition, with an Arrhenius behaviour, giving a value for the activation energy of Ea = 149 ± 4 kJ/mol. Young’s modulus was measured on all the resulting processed composites. The fall of Young’s modulus with dissolution time and temperature was also found to follow time–temperature superposition, with an Arrhenius behaviour giving a value for Ea = 198 ± 29 kJ/mol. The Young’s Modulus results plotted against $${v}_{m}$$

v
m

determined from the WAXS measurements fitted well to the Voigt upper bound parallel Rule of Mixtures $$.$$
.