bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of concentrate levels on the abundance of some rumen organisms in feedlot Nellore cattle determined by qPCR
Authors: Costa Ana C. , Carvalho Laura B. , Vieira Karine P. N. , Fonseca Alessandra S. R. , Abreu Matheus L. C. , Cabral Thainá P. S. , Galati Rosemary L. , Tavares José R. , Ferreira Daniela C. , Cabral Luciano da Silva 
Published: May 2023

Abstract
This study aimed to evaluate the effects of concentrate levels (CL) (45 to 90% on a dry matter basis) on the abundance of some species of cultured-rumen bacterial, protozoa, and Archaea in four rumen-fitted Nellore cattle. Species-designated primers were used to quantify rumen bacteria, while group and Domain-specific primers were used to quantify protozoa and Archaea by qPCR. CL caused a tendency of quadratic effect (p = 0.067) in the abundance of Fibrobacter succinogenes, while the abundance of Ruminococcus albus (p = 0.003) and Selenomans ruminantium (p = 0.006) presented a linear increase according to CL. Prevotella ruminicola abundance was quadratically affected by CL (p = 0.005), and Streptococcus bovis demonstrated a tendency of linear decrease (p = 0.076). There was no effect of CL in the abundance of Archaea (p = 0.248), but there was a quadratic effect in the abundance of protozoa (p = 0.033). The increase in CL from 45 to 90% for feedlot cattle promoted significant changes in the abundance of some species of bacteria and protozoa. However, these changes were more abrupt than reported in the literature because the changes in the diets were made progressively. Thus, a progressive increase in CL seems to cause few disturbances in the abundance of cultured rumen bacteria.
Multiplex movie of concerted rotation of molecules on a 2D material
Authors: Scholz Markus , Baumgärtner Kiana , Nozaki Misa , Reuner Marvin , Wind Nils , Haniuda Masato , Metzger Christian , Heber Michael , Kutnyakhov Dmytro , Pressacco Federico , Wenthaus Lukas , Hara Keisuke , Min Chul-Hee , Beye Martin , Reinert Friedrich , Roth Friedrich , Mahatha Sanjoy , Madsen Anders , Wehling Tim , Niki Kaori , Popova-Gorelova Daria , Rossnagel Kai 
Published: May 2023

Abstract
Function is dynamic and originates at atomic interfaces. Combining the degrees of freedom of molecules (1,2) with the peculiar properties of 2D quantum materials (3,4,5) can create novel functionality (6,7). Here, we report the manipulation and ultrafast imaging of a unidirectional gearing motion in molecules on a 2D quantum material. To visualize and disentangle the intertwined structural and electronic dynamics of such a hybrid interface, we record a ‘full molecular movie’ (8) by imaging the atomic positions (9,10), the evolution of the molecular orbital wavefunctions (11,12) and the modification of electronic states of the substrate (13). In a multimodal investigation in a single setup (14), we disentangle dynamics in valence and core electrons of both the molecule and the surface with femtosecond and sub-ångström precision. The ultrafast rotational motion is fueled by the transfer of hot holes into the molecules that results in “supercharging” of the film. As hot carriers move through the interface, we track a transient modification of the frontier molecular orbitals and observe a chiral symmetry breaking associated with local structural rearrangements. Our calculations show that the “supercharging” changes the interfacial potential energy landscape and triggers the gearing motion. The experiment offers all-in-one imaging of the electronic, molecular orbital, chemical and structural dynamics during the flow of charge and energy across the hybrid interface. Our approach provides detailed dynamical information on the mechanism underlying surface-adsorbed molecular gears and enables tailoring novel functionalities in hybrid active matter.
Identification of the Best Sector for Multinatinal Companies Investment Using the Q-rung Double Hierarchy Linguistic Term Set Todim Approach
Authors: HONGBIN YING , Gulistan Muhammad , MAHMOOD MUBASHAR , Khurshid Adnan , Rafique Amir , Al-Shamiri Mohammed M. 
Published: May 2023

Abstract
The Multinational Companies started with the rise of colonialism in the world. As a major drive of colonialism, the early multinational corporations built "factories" in the port cities of colonized countries. Now, Multinational Companies (MNCs) are investing in different sectors of the economy. However, asset allocation decisions still require proper investigation of industries and countries where the MNCs must invest. Moreover, decision-making becomes more difficult due to incomplete data, uncertainty, psychological and cultural issues in different countries, and hesitancy of the decision-makers (DMs). To overcome these issues, sophisticated decision-making techniques must be employed, so we propose the TODIM approach under the environment of q-rung orthopair double hierarchy linguistic term set (q-RODHLTS), which is the hybrid of q-rung orthopair fuzzy set and double hierarchy linguistic term set.
Further, we define basic operations like addition, multiplication, scalar multiplication, exponential, and the comparison functions and distance of q-RODHLT sets. Finally, some applications related to the different sectors for investment by a multinational company (MNC), such as Tourism, energy, and education, are presented using the proposed approaches with comparative analysis. We conclude that energy is the best sector for investment by the MNCs.
1991 Mathematics Subject Classification. 03E72, 03B52, 90C70, 47S40, 15B15.
Polymyositis in a Patient with COVID-19 Infection with Colorectal Cancer: A Case Report
Authors: Risal Ujjwol Prasad , Pandey Suravi , Regmi Pradeep Raj , Shrestha Bigya 
Published: May 2023

Abstract

Background: Association of idiopathic inflammatory myopathies (IIM) with malignancy has been well- recognized for a long time. However, its association with the novel corona virus (COVID-19) infection is extremely rare. Here we report a case of polymyositis in a patient with COVID-19 infection with underlying colorectal cancer.
Case Presentation: Here we report a case of a 73-year-old male who was admitted for COVID-19 infection but developed persistent limb weakness which was finally diagnosed as polymyositis. He was given high dose steroid along with methotrexate to which he responded very well.
Conclusions: Inflammatory myopathy should be suspected in elderly patients who present with generalized weakness especially in the background of malignancy. The association of COVID-19 infection with myositis needs further studies.
Laboratory and in-situ Selenium Bioaccumulation Assessment in the Benthic Macroinvertebrates Hyallela azteca and Chironomus dilutus
M. P. Mendes1, B. Cupe-Flores1, &amp; K. Liber 1,2
(1) Toxicology Centre, University of Saskatchewan, Canada
(2) School of Environment and Sustainability, University of Saskatchewan, Canada
Authors: Mendes Maira Peixoto , Flores Beatriz Cupe , Liber Karsten 
Published: May 2023

Abstract
Selenium (Se) bioaccumulation and toxicity in aquatic vertebrates have been thoroughly investigated over the past two decades. Limited information is available on Se bioaccumulation at the base of aquatic food webs. In this study, we selected two laboratory-reared benthic macroinvertebrates (BMI), Hyalella azteca and Chironomus dilutus, to investigate Se bioaccumulation potential in a Canadian boreal lake (McClean Lake) receiving continuous Se input from a Saskatchewan uranium mill. Laboratory and in-situ experiments were conducted in summer 2019 to assess the potential effects of biological competition on Se uptake and transfer in co-exposed H. azteca and C. dilutus. In 2021, additional experiments were included to examine the use of lab-reared H. azteca as an indicator of on-site Se exposure (native population vs lab-reared) and the major source of Se (surface water, top 1 cm and top 2-3 cm sediment layers) contributing to Se bioaccumulation in H. azteca. Except for one site (9), comparable Se bioaccumulation and trophic transfer factors (TTFs) were observed in co-exposed H. azteca (whole-body Se 0.9 to 3.1 µg /g d.w; TTFs 0.6 to 6.3) and C. dilutus (whole-body Se at 0.7 to 3.2 µg Se/g d.w.; TTFs 0.7 to 3.4). Similar non-linear dietary Se uptake and bioaccumulation were observed between lab-reared and native H. azteca (NLR, p=0.003; 4.1 ± 0.8 µg Se/g d.w) until day 14 of the experiment. Results from this study corroborate the importance of the sediment-detrital pathway relative to waterborne Se exposure, with the former leading to greater Se bioaccumulation potential to higher trophic levels via BMI.
Disrupted Identities, Invisibility and Precarious Support: A Mixed Methods Study of LGBTQI Adolescents and Young Adults with Cancer
Authors: Ussher Jane M , Allison Kimberley , Power Rosalie , Ryan Samantha , Perz Janette , Team The Out with Cancer Study 
Published: May 2023

Abstract
Background Lesbian, gay, bisexual, transgender, queer and intersex (LGBTQI) adolescents and young adults (AYAs) report higher levels of distress than non-LGBTQI AYAs with cancer, and LGBTQI adults with cancer. This mixed methods study examined LGBTQI AYA’s experiences of cancer and cancer care, to understand this distress.Methods Online surveys were completed by 95 LGBTQI AYAs (age 16–39 years); 19 AYAs took part in a one-to-one semi structured interview. Reflexive thematic analysis of interviews and open-ended survey responses facilitated in-depth examination of subjective experiences; descriptive statistics performed on closed-ended survey items identified the percentage of AYAs reporting experiences identified in the qualitative analysis.Results 63% of AYAs reported high or very high distress. Three themes identified in the qualitative analysis included: 1) “Identities in flux”, included subthemes “Cancer disrupts developing identities, and involvement with LGBTQI communities”; “Internalized prejudice impacts identities”; and “Cancer facilitates identities and embodiment”. 2) “Invisibility in cancer care”, included subthemes “Navigating disclosure amongst cis-heteronormative assumptions”, “Discrimination and paternalistic cancer care” and “Cis-heteronormativity within cancer information”. 3) “Precarious social support for LGBTQI AYAs with cancer”, included subthemes “Social support during cancer is helpful for LGBTQI AYAs”, “LGBTQI AYAs navigate limited support”, and “Finding cancer peer support networks is difficult for LGBTQI AYAs”.Conclusions LGBTQI AYAs with cancer experience psychosocial vulnerabilities related to identity development, experiences of care, and social support networks. These factors likely contribute to their previously evidenced elevated risk of distress, relative to both non-LBGTQI AYAs and LGBTQI older adults. AYAs affected by cancer may require additional, tailored supportive care, including targeted information resources, LGBTQI AYA specific cancer support groups, or partnerships and referrals to LGBTQI community organisations. Additionally, it is evident that health care professionals and cancer services have much work to do in ensuring LGBTQI AYAs receive affirming and appropriate care across paediatric and adult clinical settings. They must move beyond assuming all patients are cisgender, heterosexual and do not have intersex variations unless otherwise stated; work to signal inclusivity and facilitate disclosure; and be able to respond appropriately with tailored information and care, which is inclusive of LGBTQI partners, chosen family, and support systems.
The relation between the surface composition anomaly and distribution of the exosphere of Mercury
Authors: Suzuki Yudai , Yoshioka Kazuo , Murakami Go , Yoshikawa Ichiro 
Published: May 2023

Abstract
In celestial bodies with tenuous collisionless atmospheres, such as Mercury, the spatial distribution of the exosphere is expected to reflect the surface composition. In this study, we discuss whether the distributions of Mg, Ca, and Na, the primary exospheric components of Mercury, have an exosphere-surface correlation by analyzing the observation data of the Mercury Atmospheric and Surface Composition Spectrometer (MASCS) and X-Ray Spectrometer (XRS) onboard the MErcury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) spacecraft. It was found that Mg has a strong exosphere-surface correlation and Ca has a weak correlation. The Monte Carlo simulations of trajectories in the exosphere show that the weak correlation of Ca is due to the relatively large solar radiation acceleration. In addition, Na production rate in the high-temperature regions is longitudinally dependent. This can be explained by considering that the weakly physisorbed Na layer on the surface is depleted under high temperature and that the distribution of strongly chemisorbed Na atoms is reflected in the exosphere. Based on these results, the conditions for components with a correlation in celestial bodies with thin atmospheres may include low volatility and low solar radiation acceleration.
Novel Long non-coding RNA MAGEA6-DT1 Promotes Expression of the Melanoma Antigen Family 6 by Demethylating its Enhancer
Authors: Hao Jun , Wang Yangjunqi , Liu Wei , Yuan Wenjun , Jia Jun , Li Shuying , Yang Haisheng , Peng Shiyuan , Xie Fei , Zhong Rugang , Hao Yanzhe , Li Jintao 
Published: May 2023

Abstract
Background The occurrence and progression of various solid tumors are associated with the melanoma-associated antigen A (MAGE-A) family. Although it was demonstrated that demethylation at the promoter region usually causes the over-expression of the MAGE-A family, there has been very few research about the detailed mechanisms of how the genetic modification of promoter region promotes MAGE-A expression.Methods A new non-coding RNA (ncRNA) with the ability of binding with melanoma-associated antigen-A6 (MAGE-A6) promoter region was discovered. The expression consistency between MAGE-A6 and this novel ncRNA in different MAGE-A6 highly expressed malignant cell lines was analyzed by RT-qPCR. The full length of this ncRNA was acquired through RACE and were subsequently named as MAGEA6-DT1. Then up- and down-regulation of MAGEA6-DT1 in human malignant melanoma cells were achieved by lentivirus transduction and siRNA transfection respectively and the transcription and expression of MAGE-A6 was detected by RT-qPCR and Western Blot for verifying MAGE-A6 expression regulating function of MAGEA6-DT1. The exact binding site of MAGEA6-DT1 in MAGE-A6 promoter region was analyzed by dual-luciferase reporter system assay after MAGEA6-DT1 transfection in 293T cells. Moreover, by DNA methylation analysis, we tested whether MAGEA6-DT1 has the ability of MAGE-A6 expression regulation by manipulating its promoter region’s methylation. Finally, RNA pull-down assay was performed to identify the functional binding partner of MAGEA6-DT1.Results MAGEA6-DT1 was identified as a long non-coding RNA (lncRNA) with the length of 771 nucleotides and was abnormally expressed in consistency with MAGE-A6 among various cancer cell lines. Manipulation of MAGEA6-DT1 expression level would positively regulates MAGE-A6 expression. Specific binding site of MAGEA6-DT1 located near the enhancer of MAGE-A6, and its function was revealed to demethylate DNA near its binding site, probably with the assistance of relevant binding partners.Conclusion MAGEA6-DT1, as a lncRNA abnormally expressed in different malignant cell lines, could positively regulate MAGE-A6 expression via specifically combining with and subsequently demethylating MAGE-A6 enhancer. This function may be assisted by some of its binding protein such as DNA (cytosine-5)-methyltransferase 1 (DNMT1).
A novel Corchorus olitorius-derived biochar/Bi 12 O 17 Cl 2 photocatalyst for decontamination of antibiotic wastewater containing tetracycline under natural visible light
Authors: Samy Mahmoud , Alalm Mohamed Gar , abodlal Ribh S. , El-Dissouky Ali , Khalil Mohamed N. , El-Helow Ehab R , Khalil Tarek , Tawfik Ahmed 
Published: May 2023

Abstract
Herein, a novel composite of Corchorus olitorius-derived biochar and Bi12O17Cl2 was fabricated and utilized for the degradation of tetracycline (TC) in a solar photo-oxidation reactor. The morphology, chemical composition, and interaction between the composite components were studied using various analyses. The biochar showed a TC removal of 52.7% and COD mineralization of 59.6% using 150 mg/L of the biochar at a pH of 4.7±0.5, initial TC concentration of 163 mg/L, and initial COD of 1244 mg/L. The degradation efficiency of TC increased to 63% and the mineralization ratio to 64.7% using 150 mg/L of bare Bi12O17Cl2 at a pH of 4.7±0.5, initial TC concentration of 178 mg/L, and COD of 1034 mg/L. In the case of biochar/Bi12O17Cl2 composite, the degradation efficiency of TC and COD mineralization ratio improved to 85.8% and 77.7% due to the potential of biochar to accept electrons which retarded the recombination of electrons and holes. The synthesized composite exhibited high stability over four succeeding cycles. According to the generated intermediates, TC could be degraded to caprylic acid and pentanedioic acid via the frequent attack by the reactive species. The prepared composite is a promising photocatalyst and can be applied in large-scale systems due to its high degradation and mineralization performance in a short time besides its low cost and stability.
Morpho-phytochemical Screening and Molecular Diversity of Pomegranate Accessions Grown in Halabja Governorate, Kurdistan Region-Iraq
Authors: Faraj Jamal Mahmood 
Published: May 2023

Abstract
Halabja governorate in the Kurdistan region-Iraq is famous for having high-quality pomegranate accessions. The current study was conducted to evaluate the morpho-phytochemical amount of pomegranate functional food and the genetic diversity as an important tool for the characterization of the genetic resources for germplasm management and the identification of the best genotypes for genetic improvement. In this study, phenotypical parameter, highly significant morphometric differences (P ≤ 0.05) among 24 genotypes, with the mean values of fruit weight, peel thickness, and weight of 100 Arils, total flavonoid content (TFC) and total phenolic content (TPC), antioxidant activity, total soluble solid content (TSS) 299.21 g, 3.47 mm and 38.59 g, 54.50,47.97 µg/ml, 21.08 µg/ml and 14.59 Brix respectively. In addition, inter-simple sequence repeat (ISSR) was utilized to assess the genetic diversity of the collected pomegranate genotypes. Twelve random primers of produced products plus a number of the amplified primer bands ranging from 3 to 12 and total number of 83 amplified bands were produced, among which 78 bands were polymorphic and 5 bands were monomorphic. The highest, lowest, and mean values of polymorphic bands were (11, 3, and 6.5), respectively. The PIC values ranged from 0.58 to 0.90. The dendrogram clusters for all the selections showed dissimilarity coefficients ranging between 0.22 to 0.23 (G4 vs. G5) to 0.63 (G13 vs. G14), and five groups (A, B, C, D, and F) with a mean dissimilarity (0.49). According to the results, morphometric and biochemical properties are significant aspects of development, discernment, and classification. Alongside with the ISSR marker, which is a useful technique for allowing the identification of different selections and assessing the genetic similarity among studied pomegranate accessions, this would facilitate their use as identified genetic stock in future breeding programs.
Evaluating the effect of the COVID-19 virus on the wind energy sectors
Authors: Jamali Riaz Hussain , Bughio Amanullah , Tunio Raza Ali , Das Ghansham 
Published: May 2023

Abstract
The purpose of this study is to determine during the COVID-19 epidemic effects on wind and green energy and control the raising the cost of utilizing wind energy to power for country energy plants using the Levelized Cost of Energy methods. Objective 1) The COVID-19 pandemic can be provided through green financial policies such as coal pricing, transferable green certificates, and loans for wind energy markets. Objective 2) examined the cost of wind energy in china before and after the COVID-19 outbreak, using data from 100 wind energy projects constructed between 2014 and 2020. Based on results, wind energy's fixed average cost of electricity fell from 0.98 Chinese yuan in 2014 to 0.79 Chinese Yuan in March 2019, and subsequently to 0.75 Chinese Yuan in 2020, a 13.99 percent increase. Other results average electricity generation price down to 0.79 Yuan, 0.99 Yuan, and 0.79 Yuan and average carbon oxide emissions was 50 Yuan/ton increase. The green fiscal policies will be required during the COVID-19 epidemic to promote wind energy generation investment.
Deep learning model based on CNN-former in the diagnosis and detection of liver fibrosis
Authors: Han Jing-Jing , Zhang Xia , Cai Xiao , Pan Rui , Xiao Li , Nan Yuchen , Zhang Peng-Cheng , Yang Yong-Lin 
Published: May 2023

Abstract
Background: Hepatic fibrosis is a common pathological process in the process of various liver injury and repair. It can develop into advanced cirrhosis or even liver cancer under the stimulation of various factors. Early diagnosis and intervention can significantly improve liver function and even reverse the histopathological process. Accurate non-invasive identification and staging of early liver fibrosis has become the research goal of many scholars.
Objective: This study aimed to propose a model training method and system for liver fibrosis lesion detection based on CNN-Former.
Study design: We first collect the clinical patient data and introduce the setup and data preprocessing of the experimental data, then introduce the experimental process of the feature engineering , the ablation experiment conducted and the evaluation index . Finally, compared this method with traditional method .
Results: By modeling the physiological and biochemical characteristics of patients with liver fibrosis, the pre-detection of liver fibrosis is carried out with high efficiency, high quality and high standard, providing a solution for existing research to rely on image information. In addition, on the basis of the existing CNN-form model, multi-CNN-form adds a multi-task mechanism to enable the model to predict liver fibrosis and related complications at the same time, providing a solution to the problem that traditional models focus on specific diseases and poor transplantation. Also, the accuracy, recall, AUC value, F1 value and other evaluation indexes of the model were calculated based on the test set and confusion matrix to compare the effects of various models in predicting liver fibrosis pre-detection. Finally, the effectiveness of this method compared with the traditional method is demonstrated from many angles. This makes it more suitable in practical application scenarios.
Conclusions: This paper mainly introduces a liver fibrosis pre-detection method based on deep learning, that is, the liver fibrosis pre-detection method based on CNN-former, which provides a solution for the existing research to rely on imaging information. In addition, the pre-detection method of liver fibrosis based on multi-CNN-former provides a solution to the problem of focusing on specific diseases and poor transplantation of traditional models.