bluuebunny/crossref_metadata_2025_split huggingface dataset:
STUDENTS’ PERCEPTION OF LEARNING MANAGEMENT SYSTEM ON STUDENTS’ STUDY HABIT DURING COVID-19
Authors: Balogun Naeem Atanda , Isimikalu I Mary , Adedoyin Adeyinka 
Published: March 2023

The study investigated perception of students on Learning Management System and how it affects and benefits their study habit during COVID-19, as well as its effects on their study performance. The study adopted quantitative research approach and survey design where data was gathered using a questionnaire that was created to acquire pertinent information from respondents. The study participants are the 200 and 300 level students of Faculty of Education, University of Ilorin, while a total of 358 students administered the questionnaire only 303 responses are used for the study. The results demonstrated that students' study habits are significantly impacted by the use of Learning Management Systems (LMS) as majority of the respondents attest that the use of LMS improved their study habit which in turn affected their study result positively. These could also assist lecturers and university administration to know how well students are able to relate with Learning Management System, how it affects their academic performance and their preparedness to fully adopt the system.
Penerapan Metode Geolistrik Resistivitas Konfigurasi Wenner Untuk Mengetahui Dugaan Rembesan Polutan Sampah Di Sekitar Tempat Pembuangan Akhir (TPA) Desa Tanjung Belit Kabupaten Rokan Hulu
Authors: Daruwati Ika , Demulawa Meilan 
Published: March 2023

A resistivity geoelectric research has been carried out to identify the suspected seepage of garbage pollutants around the Tanjung Belit village garbage dump. The study was conducted in 2 tracks. On track 1 with a track length of 100 m and 35 measurement points, the resistivity is in the range of 0.795-50.1 Ωm. On the 2nd track, 75 m long and 32 measuring points. The method used is the geoelectric resistivity method of the Wenner configuration with data processing using the res2dinv application which has a large resistivity ranging from 1.22-44.9 Ωm. Based on this resistivity, it can be seen that there is waste pollutant seepage located at a depth of 0.825–7.41 m.

Keywords:Wenner, geolistric method, RES2DINV
Korelasi Perilaku Merokok Dengan Kesehatan Masyarakat Desa Tegal Mukti
Authors: Kamelia Marlina , Marmanik Tantri Subo , Satiyarti Rina Budi , Widiani Nurhaida , Novitasari Aulia 
Published: March 2023

Masyarakat Indonesia masih banyak yang memiliki pola hidup tidak sehat. Pola hidup tidak sehat yang kerap dilakukan salah satunya adalah kebiasaan merokok. Rokok memiliki kandungan gas dan partikel yang berbahaya bagi kesehatan apabila dikonsumsi secara terus menerus serta dalam jumlah banyak. Hal ini karena zat yang terkandung dalam rokok tersebut bersifat genotoksik sehingga bisa memicu penyakit degeneratif. Setidaknya ada dua jenis rokok yaitu filter dan kretek. Jenis rokok ini memiliki perbedaan pada kandungan nikotinnya serta kemampuannya menyaring zat-zat berbahaya. Tujuan penelitian ini adalah untuk mengetahui korelasi perilaku merokok dengan kesehatan masyarakat. Metode penelitian ini adalah observasional analitik dengan pendekatan cross sectional. Responden yang terlibat berjumlah 68 dengan mempertimbangkan kriteria inklusi. Parameter yang diukur meliputi usia, tekanan darah, frekuensi denyut nadi, frekuensi pernafasan, dan saturasi oksigen. Hasil penelitian menunjukkan jenis rokok berkorelasi nyata terhadap karakteristik responden berupa usia, tekanan darah, frekuensi pernapasan, serta saturasi oksigen namun tidak berpengaruh nyata pada frekuensi denyut nadi. Derajat merokok berkorelasi terhadap karakteristik responden pada tekanan darah, frekuensi denyut nadi, frekuensi pernapasan, dan saturasi oksigen namun tidak dengan usia. Berdasarkan data menunjukkan derajat merokok yang semakin tinggi berpotensi menurunkan kualitas kesehatan perokok tersebut.
PENGARUH UMUR, PARITAS DAN PENGGUNAAN ALAT KONTRASEPSI TERHADAP KEJADIAN DISFUNGSI SEKSUAL
Authors: Permatai Maryati , Sakung Jamaludin , Gunarmi Gunarmi , Pramana Cipta 
Published: March 2023

Disfungsi seksual termasuk gangguan keinginan, gairah seksual, lubrikasi, orgasme, dan rasa sakit. Tujuan penelitian untuk mengetahui pengaruh umur, paritas dan penggunaan alat kontrasepsi Terhadap Kejadian Disfungsi Seksual Di Puskesmas Sulewana Kabupaten Poso. Penelitian menggunakan desain Cross-Sectional Study. Responden dalam penelitian ini adalah Pasangan usia subur (PUS) berumur 20-46 tahun yang berada di Puskesmas Sulewana tahun 2020 Pengguna KB hormonal DMPA non hormonal analisis data untuk menentukan pengaruh variabel dependen dengan independen menggunakan uji regresi logistik. Hasil analisis menunjukkan dari 69 responden yang menggunakan kontrasepsi hormonal menunjukkan 67 diantaranya mengalami disfungsi seksual (97,1%), dan 2 diantaranya normal (2,9%). Sedangkan dari 26 responden yang menggunakan kontrasepsi non hormonal menunjukkan 18 diantaranya mengalami disfungsi seksual (69,2%) dan 8 diantaranya normal (30,8%). Berdasarkan hasil uji regresi logistik diperoleh nilai nilai pvalue = 0,001 (p-value < 0,05), hal ini menunjukkan ada pengaruh yang bermakna penggunaan alat kontrasepsi terhadap kejadian disfungsi seksual di puskesmas Sulewana kabupaten Poso, dengan koefisien determinan = 0,282. Berdasarkan uji statistik diperoleh umur dan paritas tidak mempengaruhi terhadap disfungsi seksual, sedangkan penggunaan alat kontrasepsi berpengaruh terhadap kejadian disfungsi seksual di puskesmas Sulewana Kabupaten Poso.
Financial Literacy Ability in Solving Mathematical Problems (Case Study of Bugisneese Gender Differences)
Authors: Wangsa Arma , Retnawati Heri , Wijaya Ariyadi , Iman Asrul Nur , Umasugi Sitti Mutia 
Published: March 2023

This study aims to analyze students' financial literacy abilities in terms of Bugisneese gender differences (oroane, makkunrai, calabai, and calalai) in solving math problems using a survey method of 309 XI graders of public senior high schools in Soppeng Regency, South Sulawesi Province. The data collection techniques were carried out using a financial literacy test and gender diversity questionnaires. The collected data were analyzed using ANOVA with the help of IBM SPSS Statistics 25 (trial version). The research results showed that there were differences in students' financial literacy abilities, especially between students with an oroane and makkunrai gender tendency. The financial literacy abilities of students who tend to gender with feminine roles (makkunrai and calabai) are better than students who tend to gender with masculine roles (oroane and calalai). These results construct the knowledge that differences in students' financial literacy abilities in terms of solving mathematics problems do not only occur in sex differences (binary gender roles) cases but also involve differences in non-binary gender roles. Therefore, gender role differences need to be considered in integrating learning mathematics with financial literacy.
Antimicrobial, anticancer, and biofilm inhibition studies of highly reduced graphene oxide (HRG): In vitro and in silico analysis
Authors: Alangari Abdulaziz , Mateen Ayesha , Alqahtani Mohammed S. , Shahid Mudassar , Syed Rabbani , Shaik Mohammed Rafi , Khan Mujeeb , Adil Syed Farooq , Kuniyil Mufsir 
Published: March 2023

Background: Bacterial infections and cancers may cause various acute or chronic diseases, which have become serious global health issues. This requires suitable alternatives involving novel and efficient materials to replace ineffective existing therapies. In this regard, graphene composites are being continuously explored for a variety of purposes, including biomedical applications, due to their remarkable properties.Methods: Herein, we explore, in-vitro, the different biological properties of highly reduced graphene oxide (HRG), including anti-cancer, anti-bacterial, and anti-biofilm properties. Furthermore, to analyze the interactions of graphene with proteins of microbes, in silico docking analysis was also carried out. To do this, HRG was prepared using graphene oxide as a precursor, which was further chemically reduced to obtain the final product. The as-prepared HRG was characterized using different types of microscopic and spectroscopic techniques.Results: The HRG revealed significant cytotoxic ability, using a dose-dependent anti-cell proliferation approach, which substantially killed human breast cancer cells (MCF-7) with IC50 of 29.51 ± 2.68 μg/mL. The HRG demonstrated efficient biological properties, i.e., even at low concentrations, HRG exhibited efficient anti-microbial properties against a variety of microorganisms. Among the different strains, Gram-positive bacteria, such as B. subtilis, MRSA, and S. aureus are more sensitive to HRG compared to Gram-negative bacteria. The bactericidal properties of HRG are almost similar to a commercially available effective antibiotic (ampicillin). To evaluate the efficacy of HRG against bacterial biofilms, Pseudomonas aeruginosa and MRSA were applied, and the results were compared with gentamycin and ampicillin, which are commonly applied standard antibiotics. Notably, HRG demonstrated high inhibition (94.23%) against P.aeruginosa, with lower MIC (50 μg/mL) and IC50 (26.53 μg/mL) values, whereas ampicillin and gentamicin showed similar inhibition (90.45% and 91.31% respectively) but much higher MIC and IC50 values.Conclusion: Therefore, these results reveal the excellent biopotential of HRG in different biomedical applications, including cancer therapy; antimicrobial activity, especially anti-biofilm activity; and other biomedicine-based therapies. Based on the molecular docking results of Binding energy, it is predicted that pelB protein and HRG would form the best stable docking complex, and high hydrogen and hydrophobic interactions between the pelB protein and HRG have been revealed. Therefore, we conclude that HRG could be used as an antibiofilm agent against P. aeruginosa infections.
Mesenchymal stem cell-derived secretome enhances nucleus pulposus cell metabolism and modulates extracellular matrix gene expression in vitro
Authors: Tilotta Veronica , Vadalà Gianluca , Ambrosio Luca , Cicione Claudia , Di Giacomo Giuseppina , Russo Fabrizio , Papalia Rocco , Denaro Vincenzo 
Published: March 2023

Introduction: Intradiscal mesenchymal stromal cell (MSC) therapies for intervertebral disc degeneration (IDD) have been gaining increasing interest due to their capacity to ameliorate intervertebral disc metabolism and relieve low back pain (LBP). Recently, novel investigations have demonstrated that most of MSC anabolic effects are exerted by secreted growth factors, cytokines, and extracellular vesicles, collectively defined as their secretome. In this study, we aimed to evaluate the effect of bone-marrow-MSCs (BM-MSCs) and adipose-derived stromal cells (ADSCs) secretomes on human nucleus pulposus cells (hNPCs) in vitro.Methods: BM-MSCs and ADSCs were characterized according to surface marker expression by flow cytometry and multilineage differentiation by Alizarin red, Red Oil O and Alcian blue staining. After isolation, hNPCs were treated with either BM-MSC secretome, ADSC secretome, interleukin (IL)-1β followed by BM-MSC secretome or IL-1β followed by ADSC secretome. Cell metabolic activity (MTT assay), cell viability (LIVE/DEAD assay), cell content, glycosaminoglycan production (1,9-dimethylmethylene blue assay), extracellular matrix and catabolic marker gene expression (qPCR) were assessed.Results: 20% BM-MSC and ADSC secretomes (diluted to normal media) showed to exert the highest effect towards cell metabolism and were then used in further experiments. Both BM-MSC and ADSC secretomes improved hNPC viability, increased cell content and enhanced glycosaminoglycan production in basal conditions as well as after IL-1β pretreatment. BM-MSC secretome significantly increased ACAN and SOX9 gene expression, while reducing the levels of IL6, MMP13 and ADAMTS5 both in basal conditions and after in vitro inflammation with IL-1β. Interestingly, under IL-1β stimulation, ADSC secretome showed a catabolic effect with decreased extracellular matrix markers and increased levels of pro-inflammatory mediators.Discussion: Collectively, our results provide new insights on the biological effect of MSC-derived secretomes on hNPCs, with intriguing implications on the development of cell-free approaches to treat IDD.
Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis
Authors: Chen Jianfu , Yang Yuan , Li Yunyan , Xu Lirong , Zhao Chun , Chen Qi , Lu Yongping 
Published: March 2023

Introduction: The side effects of conventional therapy for acute deep vein thrombosis (DVT) are severe, with inflammatory reactions playing a pivotal role. It is particularly important to explore new ways of treatment thrombosis by targeting inflammatory factors.Methods: A targeted microbubble contrast agent was prepared using the biotin-avidin method. The 40 DVT model rabbits were established and divided into four groups according to different treatment regimens. The four coagulation indexes, TNF-α, and D-dimer content of experimental animals were measured before modeling and before and after treatment, and the thrombolysis was assessed by ultrasound imaging. Finally, the results were verified by pathology.Results and Discussion: Fluorescence microscopy verified the successful preparation of targeted microbubbles. Among the groups, PT, APTT, and TT in Group II-IV were longer than those in Group I (all p &lt; 0.05). FIB and D-dimer content were lower than those in Group I (all p &lt; 0.05), and TNF-α content in Group IV was lower than that in Group I-III (all p &lt; 0.05). Pairwise comparison before modeling and before treatment and after treatment showed that, after treatment, the PT, APTT, and TT in Group II-IV were longer than those before modeling (all p &lt; 0.05). The contents of FIB and D-dimer were lower than those before modeling and before treatment (all p &lt; 0.05). The content of TNF-α decreased significantly only in Group IV, but increased in the other three groups. Targeted microbubbles combined with Low-power focused ultrasound can reduce inflammation, significantly promote thrombolysis, and provide new ideas and methods for the diagnosis and treatment of acute DVT.
Mutagenic analysis of actin reveals the mechanism of His161 flipping that triggers ATP hydrolysis
Authors: Iwasa Mitsusada , Takeda Shuichi , Narita Akihiro , Maéda Yuichiro , Oda Toshiro 
Published: March 2023

The dynamic assembly of actin is controlled by the hydrolysis of ATP, bound to the center of the molecule. Upon polymerization, actin undergoes a conformational change from the monomeric G-form to the fibrous F-form, which is associated with the flipping of the side chain of His161 toward ATP. His161 flipping from the gauche-minus to gauche-plus conformation leads to a rearrangement of the active site water molecules, including ATP attacking water (W1), into an orientation capable of hydrolysis. We previously showed that by using a human cardiac muscle α-actin expression system, mutations in the Pro-rich loop residues (A108G and P109A) and in a residue that was hydrogen-bonded to W1 (Q137A) affect the rate of polymerization and ATP hydrolysis. Here, we report the crystal structures of the three mutant actins bound to AMPPNP or ADP-Pi determined at a resolution of 1.35–1.55 Å, which are stabilized in the F-form conformation with the aid of the fragmin F1 domain. In A108G, His161 remained non-flipped despite the global actin conformation adopting the F-form, demonstrating that the side chain of His161 is flipped to avoid a steric clash with the methyl group of A108. Because of the non-flipped His161, W1 was located away from ATP, similar to G-actin, which was accompanied by incomplete hydrolysis. In P109A, the absence of the bulky proline ring allowed His161 to be positioned near the Pro-rich loop, with a minor influence on ATPase activity. In Q137A, two water molecules replaced the side-chain oxygen and nitrogen of Gln137 almost exactly at their positions; consequently, the active site structure, including the W1 position, is essentially conserved. This seemingly contradictory observation to the reported low ATPase activity of the Q137A filament could be attributed to a high fluctuation of the active site water. Together, our results suggest that the elaborate structural design of the active site residues ensures the precise control of the ATPase activity of actin.
Maternal vaginal fluids play a major role in the colonization of the neonatal intestinal microbiota
Authors: Xie Jingxian , Tang Chen , Hong Shouqiang , Xin Yuntian , Zhang Jie , Lin Yi , Mao Lindong , Xiao Yunshan , Wu Quanfeng , Zhang Xueqin , Shen Heqing 
Published: March 2023

BackgroundCaesarean section (CS) is associated with newborns’ health risks due to the blocking of microbiome transfer. The gut microbiota of CS-born babies was different from those born vaginally, which may be attributed to reduced exposure to maternal vaginal microbes during labour. To understand the microbial transfer and reduce CS disadvantages, the effect of vaginal microbiota exposure on infant gut microbiota composition was evaluated using 16s rDNA sequencing-based techniques.ResultsPregnant women were recruited in the Women and Children’s Hospital, School of Medicine, Xiamen University from June 1st to August 15th, 2017. Maternal faeces (n = 26), maternal vaginal fluids (n = 26), and neonatal transitional stools (n = 26) were collected, while the participants underwent natural delivery (ND) (n = 6), CS (n = 4) and CS with the intervention of vaginal seedings (I) (n = 16). 26 mothers with the median age 26.50 (25.00-27.25) years showed no substantial clinical differences. The newborns’ gut microbiota altered among ND, CS and I, and clustered into two groups (PERMANOVA P = 0.001). Microbial composition of ND babies shared more features with maternal vaginal samples (PERMANOVA P = 0.065), while the microbiota structure of ND babies was obviously different from that of sample of maternal faeces. The genus Bacteroides in CS-born babies with intervention approached to vaginal-born neonates, compared with CS-born neonates without intervention.ConclusionsNeonatal gut microbiota was dependent on the delivery mode. And the gut microbiota CS newborns with vaginal seeding shared more features with those of ND babies, which hinted the aberrant gut microbiota composition initiated by CS might be partly mitigated by maternal vaginal microbiota exposure.
Molecular mechanisms of Huanglian Jiedu decoction in treating Alzheimer’s disease by regulating microbiome via network pharmacology and molecular docking analysis
Authors: Zheng Renyuan , Shi Shenggan , Zhang Qin , Yuan Shuqin , Guo Tong , Guo Jinlin , Jiang Peidu 
Published: March 2023

BackgroundHuanglian Jiedu decoction (HLJDD) is a famous traditional Chinese medicine prescription, which is widely used in the treatment of Alzheimer’s disease (AD). However, the interaction between bioactive substances in HLJDD and AD-related targets has not been well elucidated.AimA network pharmacology-based approach combined with molecular docking was performed to determine the bioactives, key targets, and potential pharmacological mechanism of HLJDD against AD, through the regulation of microbial flora.Materials and methodsBioactives and potential targets of HLJDD, as well as AD-related targets, were retrieved from Traditional Chinese Medicine Systems Pharmacology Analysis Database (TCMSP). Key bioactive components, potential targets, and signaling pathways were obtained through bioinformatics analysis, including protein-protein interaction (PPI), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Subsequently, molecular docking was performed to predict the binding of active compounds with core targets.Results102 bioactive ingredients of HLJDD and 76 HLJDD-AD-related targets were screened. Bioinformatics analysis revealed that kaempferol, wogonin, beta-sitosterol, baicalein, acacetin, isocorypalmine, (S)-canadine, (R)-canadine may be potential candidate agents. AKT1, TNF, TP53, VEGFA, FOS, PTGS2, MMP9 and CASP3 could become potential therapeutic targets. 15 important signaling pathways including the cancer pathway, VEGF signaling pathway, and NF-κB signaling pathway might play an important role in HLJDD against AD. Moreover, molecular docking analysis suggested that kaempferol, wogonin, beta-sitosterol, baicalein, acacetin, isocorypalmine, (S)-canadine, and (R)-canadine combined well with AKT1, TNF, TP53, VEGFA, FOS, PTGS2, MMP9, CASP3, respectively.ConclusionOur results comprehensively illustrated the bioactives, potential targets, and possible molecular mechanisms of HLJDD against AD. HLJDD may regulate the microbiota flora homeostasis to treat AD through multiple targets and multiple pathways. It also provided a promising strategy for the use of traditional Chinese medicine in treating human diseases.
Association between human papillomavirus infection and common sexually transmitted infections, and the clinical significance of different Mycoplasma subtypes
Authors: A Disi , Bi Hui , Zhang Dai , Xiao Bingbing 
Published: March 2023

IntroductionHuman papillomavirus (HPV) infection, especially persistent high-risk HPV, is associated with cervical cancer. Female reproductive tract microecological disorders and lower genital tract infections have been increasingly correlated with HPV infection and cervical lesions. Due to their common risk factors and transmission routes, coinfection with other sexually transmitted infections (STIs) has become a concern. Additionally, the clinical significance of Mycoplasma subtypes appear to vary. This study aimed to assess the correlations between common STIs and HPV infection, and to investigate the clinical significance of Mycoplasma subtypes.MethodsWe recruited 1,175 patients undergoing cervical cancer screening at the Peking University First Hospital gynecological clinic from March 2021 to February 2022 for vaginitis and cervicitis tests. They all received HPV genotyping and detection of STIs, and 749 of them underwent colposcopy and cervical biopsy.ResultsAerobic vaginitis/desquamative inflammatory vaginitis and STIs (mainly single STIs) were found significantly more often in the HPV-positive group than in the HPV-negative group. Among patients with a single STI, rates of infection with herpes simplex virus type 2 or UP6 in the HPV-positive group were significantly higher than in the HPV-negative group (ORadj: 1.810, 95%CI: 1.211–2.705, P=0.004; ORadj: 11.032, 95%CI: 1.465–83.056, P=0.020, respectively).DiscussionThrough detailed Mycoplasma typing, a correlation was found between different Mycoplasma subtypes and HPV infection. These findings suggest that greater attention should be paid to detecting vaginal microecological disorders in those who are HPV-positive. Further, lower genital tract infections, including both vaginal infections and cervical STIs, are significantly more common among women who are HPV-positive and who thus require more thorough testing. Detailed typing and targeted treatment of Mycoplasma should become more routine in clinical practice.