bluuebunny/crossref_metadata_2025_split huggingface dataset:
A participatory urban governance framework: the protection of urban ecosystems’ functions as a barometer for transitioning towards sustainability.
Authors: SIMON Sandrine 
Published: May 2023

Abstract
Research into ways of helping decision-makers to better integrate the notion of sustainability when designing strategies have focused on reforming the main economic indicators (such as GDP) since at least the 1990s. From greening the accounts, efforts progressively turned into creating ‘ecosystems accounts’. The dominant approach, developed by the United Nations, led to the creation of the System of Environmental and Economic Account - Ecosystem Experimental Accounts (SEEA EEA), ‘satellites’ to the main economic accounts. Although mainly used in industrialised countries, these are starting to be more widely used. This article focuses on the urgent need to make our cities (sometimes described as a microcosm of our economic systems) more sustainable and explores to what extent ecosystems accounts could help in this endeavour. However, it approaches the issue differently from what has been done so far in that it advocates considering the city itself as a socio-ecological-economic ecosystem, with its own environmental functions, whose protection will help to identify whether urban transitions are moving towards sustainability. In addition, it explores taking social considerations into account by making the use of the urban ecosystems framework presented here participatory. It thus shows how such a decision-making tool can be used iteratively to contribute to adaptive governance for urban transitions towards sustainability and highlights how such a framework can facilitate potential partnerships and make the economy more cooperative and circular. It concludes by suggesting a practical application focused on food security and demonstrates how this is strongly linked to urban resilience and sustainability.
Bacterial Species as Causative Agents Involved in Pistachios Dieback in Iran
Authors: Tavasoli Elham , Moradi Mohammad , Hasanzadeh Nader , Khodaygan Pejman , Probst Claudia 
Published: May 2023

Abstract
Die-back of pistachio (DBP) is a major disease affecting pistachio trees in Iran and has become a serious problem in Kerman province in recent years. In the current study, the role of bacteria as causal agents of DBP was investigated under laboratory and field conditions. Samples were collected from infected pistachio orchards in Kerman province from 2015 to 2016. The ability of bacterial isolates to produce disease and vascular colonization was studied through different inoculation methods. Identification of isolates was carried out using biochemical and physiological assays, amplification of 16SrDNA region combined with partial analyses of gyrA gene. A total of 281 bacterial isolates were obtained from infected trees from which 148 induced the hypersensitivity reaction on tobacco leaves. From those, 128 isolates were able to colonize vascular tissues in sub-bark inoculations of pistachio branches under laboratory conditions. In field experiments, 24 selected isolates were able to extend in vascular tissues of pistachio branches and twigs using sub-bark and apical inoculation methods, although disease severity varied. Staphylococcus pasteuri, Bacillus pumilus, Bacillus sp., Acinetobacter radioresistens, Xanthomonas sp., Curtobacterium flaccumfaciens, Arthrobacter oxydans and Pseudomonas koreensis were identified to be involved in die-back of pistachio trees. The work presented here shows that a range of bacterial genera and species may be involved with the DBP, which may be used to improve the urgently needed management of the disease.
Growth performance and hematological parameters of broiler chickens fed gradually increasing levels of supplemental safflower seed
Authors: Rathaur Aman , Rai Dinesh Chandra , Prakash Ved 
Published: May 2023

Abstract
Fats and oils are used to enhance the palatability of feed, absorb fat-soluble vitamins, and pass digesta through the gastrointestinal tract of poultry. The goal of current study was to see how dietary supplementation of Safflower seed (SS) affected on the production and hematological parameters of broiler chickens. A total of 200 male chickens (7-day-old) were randomly distributed into 5 treatments with each 5 replicate pens of 8 chicks in a 42-day trial. Each group was randomly allocated to one of 5 dietary treatments: (0, 2.5, 5, 7.5, and 10%) SS, respectively. The experimental diets were formulated for starter (7 to 21 days) and finisher (22 to 42 days) phase. Result indicated that inclusion of SS in diet improved body weight and feed conversion ratio during 7 to 42 days (P < 0.05). The highest and lowest body weight were observed in 5% SS and 0% SS levels, respectively. The obtained results demonstrated a significantly (P < 0.01) increased content of RBC, HB, PCV, Lymphocytes, MCV, MCH, MCHC, and Platelets in the blood of broiler chickens fed with various levels of SS for five weeks. Findings suggest that, SS may use as natural energy source to increase growth performance and improve hematological parameters.
Study on the correlation between controlling nutritional status score and clinical biochemical indicators in patients with colorectal cancer
Authors: Wang Zhi , Yuan Jiayan , Zhao Sunyan , Huang Shijia , Wu Rong , Fei Fei 
Published: May 2023

Abstract
Purpose The controlling nutritional status (CONUT) score is an important tool for predicting the prognosis of colorectal cancer (CRC), but its effectiveness is relatively insufficient. This study aims to screen more effective clinical indicators as supplements to the CONUT scoring system and improve the predictive value of CRC prognosis.
Methods Retrospective collection of clinical information from all CRC patients in our unit from 2014 to 2020, and calculation of CONUT scores based on serum albumin, lymphocyte, and total cholesterol levels. Divide the included patients into three groups: the normal nutrition group (0-1), the mild malnutrition group (2-4), and the moderate to severe malnutrition group (5-12). Evaluate the correlation between CONUT score and baseline characteristics and clinical indicators.
Results This study ultimately included 5014 CRC patients. The higher the values of neutrophil (NE), monocyte (MC), eosinophil (EOS), alkaline phase, lactate dehydrogenase, carboxylic antigen in patients, as well as the lower the values of white blood cell (WBC), basil (BAS), hemoglobin, total protein, triglycerides, low density lipoprotein (LDL), aspartate transaminase, blood urea nitrogen in patients, all indicate poorer nutritional status, with statistical significance (P<0.05). The indicators significantly correlated with the CONUT score all reflect immune nutritional status, including WBC (OR=0.036, P=000), NE (OR=30.815, P=0.000), MC (OR=41.388, P=0.000), EOS (OR=27.577, P=0.000), BAS (OR=0.006, P=0.046), and LDL (OR=0.319, P=0.000).
Conclusion WBC, NE, MC, EOS, BAS, and LDL may be used as supplementary indicators in the CONUT scoring system to more effectively predict the clinical prognosis of CRC patients.
Adverse Childhood Experiences and living in the socially deprived areas in adulthood: A cross-sectional study of the nationwide data in Japan
Authors: Sasaki Natsu , Tabuchi Takahiro , Fujiwara Takeo , Nishi Daisuke 
Published: May 2023

Abstract
Objectives: This study aimed to examine whether having adverse childhood experiences (ACEs) was associated with living in a deprived area in adulthood.
Methods: The cross-sectional study was conducted by using nationwide data in 2022 of the Japan COVID-19 and Society Internet Survey (JACSIS). Participants were community dwelling people 18 years or older. ACEs were assessed by Japanese version of 15-items ACE measurement tool (ACE-J). Living condition was measured by Area Deprived Index (ADI) and Densely Inhabited District (DID) based on zip code. Multivariable logistic regression to analyze the associations between ADI and ACE 4+ was conducted, controlling for individual-level factors, such as age, sex, marital status, and education, as an additional analysis.
Results: The total of 27,967 participants were included in the analysis. The prevalence of emotional neglect, childhood poverty, and school bullying were 34.5%, 21.9%, 20.0%, respectively. More than 70.1% of the population had one or more ACE(s). The number of ACEs was associated with significantly higher risk of living in deprived area in the adulthood (p<0.001). ACEs were not associated with living in density area. The association between ADI and ACEs 4+ was non-significant after controlling the individual-level factors.
Conclusion: People with higher number of ACEs tend to live in deprived areas in adulthood. Policy makers in highly deprived areas can apply the trauma-informed approach for the community care and support, which is critical to mitigating deficit perspectives and facilitating comprehensive support for those who experience ACEs.
Anatomy of thermal unrest at a hydrothermal system: Case study of the 2021-2022 crisis at Vulcano
Authors: Bonnétat Sophie Pailot - , Rafflin Victoria , Harris Andrew , Diliberto Iole Serena , Ganci Gaetana , Cappello Annalisa , Boudoire Guillaume , Bilotta Guiseppe , Grassa Fausto , Gattuso Alessandro , Ramsey Michael 
Published: May 2023

Abstract
Hydrothermal systems can generate phreatic and/or phreatomagmatic explosions with little warning. Understanding the temporal and spatial evolution of geophysical and geochemical signals at hydrothermal systems is crucial for detecting precursors to unrest and to inform on hazard. Thermal signatures of such systems are poorly defined because data records are often too short or punctual compared to activity timescales, which can be decadal. La Fossa system of Vulcano has been monitored since the 1980s and entered a period of unrest in 2021. We assessed the thermal signature using ground- and satellite-based data with temporal and spatial scales ranging from minutes to days. While continuously-recording stations provided continuous but point-based measurements, fumarole field vent surveys and ASTER and VIIRS images allowed lower temporal resolution but synoptic records to be built. By integrating this multi-resolution data set, precursory signals to the unrest could retrospectively be placed ranging from February to June 2021. Intensity of unrest increased during summer 2021, with an onset over a few days in September 2021. By September, seismic, CO2, SO2 and geochemical metrics also indicated unrest, leading Civil Protection to raise the alert level to yellow on October 1. Heat flux, having been 4 MW in May 2019, peaked at 90 MW in September, and increased to 120 MW by March 2022. This ranked Vulcano as one of the highest intensity hydrothermal systems like Reykjanes, well ahead of Yellowstone and Nysiros We thus convolved our thermal data sets with all other monitoring data to validate a Vulcano Unrest Index (VUI) that can be potentially applied to any hydrothermal system. The VUI highlighted four stages of unrest, none of which were clear in any single data set: baseline, precursory, onset and unrest. Onset was characterized by sudden release of fluids, likely caused by failure of sealed zones that had become pressurized during the precursory phase that began possibly as early as February 2021. Unrest has been ongoing for more than 18 months, and may continue for several more years. Our understanding of this system behavior has been due to hindsight, but demonstrates how multiparametric surveys can track and forecast unrest.
Effect of zinc ions on the proliferation and differentiation of keratinocytes
Authors: Miguel Willian Moreira , Freitas-Marchi Bruna Letícia , Reigado Gustavo Roncoli , Santos Jeniffer Farias dos , Chambergo Felipe Santiago , Nunes Viviane 
Published: May 2023

Abstract
Background
Psoriasis is a chronic inflammatory disease in which there is hyperproliferation and abnormal differentiation of keratinocytes. Since high levels of KLK7, an enzyme inhibited by zinc (Zn2+) ions, are present in psoriatic lesions, we have studied the effect of zinc ions in the viability of keratinocytes, as well as in the activity of KLK5 and KLK7 and in the expression of epidermal markers.
Methods and Results
The cells were cultured in the absence or presence of Zn2+ ions (5.0, 10 and 25 µM). Cell viability was evaluated by the MTT method after during 14 days. Cell death was evaluated by flow cytometry using propidium iodide. The activity of the KLK was evaluated on the hydrolysis of synthetic substrates. Expression of involucrin, filaggrin, cytokeratins (CK) 5, 10 and 14 was evaluated by quantitative PCR. Cell incubation with Zn2+ ions did not result in significant changes in cell viability. By MTT assay, it was observed that the cultures incubated with 10 and 25 µM Zn2+ ions showed a decrease in the number of viable cells in comparison to the control. Cells cultured for 1 day in the presence of 25 µM Zn2+ ions displayed a decrease in KLK7 activity. In the presence of Zn2+ ions, it was shown an increase in the expression of CK5, 10 and 14, involucrin and filaggrin.
Conclusions
These results have shown that zinc ions can affect the differentiation of HaCat cells, contributing for future therapeutic trials related to psoriasis based on the modulation of KLK activity.
Prevalence and correlates of post-traumatic stress disorder, depression, anxiety, and insomnia symptoms among tornado-affected construction workers : a cross-sectional study.
Authors: Huang Yujie , Wu Taimin , Liu Lianzhong 
Published: May 2023

Abstract
Background
The 2021 Wuhan tornado outbreak caused significant physical and mental trauma to tornado-exposed construction workers. Our study aimed to investigate the prevalence of post-traumatic stress disorder (PTSD), depression, anxiety, and insomnia symptoms among tornado-exposed workers and to analyse the associated factors.
Methods
A total of 228 tornado-affected construction workers completed a cross-sectional survey. We used the basic information questionnaire, Essen Trauma Inventory, Generalised Anxiety Disorder-7, Patient Health Questionnaire-9, and Insomnia Severity Index to screen affected workers and analyse their correlates for possible PTSD, depression, anxiety, and insomnia symptoms using logistic regression.
Results
The prevalence rates of PTSD, depression, anxiety, and insomnia symptoms were 20.2, 30.7, 23.2, and 41.6%, respectively. Willingness to seek psychological help from workers was rarely reported (18.0%). Multiple logistic regression showed that PTSD symptoms were more likely to occur with physical injury (OR = 3.05, P = 0.006), severe damage to property (OR = 2.77, P = 0.005), and witnessing death (OR = 2.34, P = 0.046). Depressive symptoms were more likely to occur with physical injury (OR = 3.292, P = 0.000) and severe damage to property (OR = 1.911, P = 0.032). Anxiety symptoms were significantly associated with physical injury (OR = 4.116, P = 0.009). Insomnia symptoms were significantly associated with severe damage to property [OR = 2.077, P = 0.030) and hospital admission (OR = 3.307, P = 0.005).
Conclusions
After the tornado, several construction workers developed psychological problems. To mitigate the psychological trauma caused by disasters, more intensive psychological monitoring and essential mental intervention should be provided to victims who have experienced physical injury, property damage, hospitalisation, and witnessed deaths.
The Significance of Position in Percutaneous Nephrolithotomy
Authors: KÜÇÜKYANGÖZ MUSTAFA , Gücük Adnan 
Published: May 2023

Abstract
Purpose
Percutaneous nephrolithotomy (PNL) can be performed in a number of different positions. Our aim was to evaluate the convenience and advantages of the supine position following the transition from the prone to the supine position.
Materials and Method
94 patients undergoing supine PNL (group 1) and 93 patients undergoing prone PNL (group 2) were retrospectively reviewed and included in the study. Patients who underwent mini-PNL (mPNL) were selected from group 1 and group 2 and divided into Group-1A supine mPNL patients (77) and Group-2A prone mPNL patients (53). Demographic characteristics, operative data and post-operative parameters were compared between these groups.
Results
The operation time was 74.4 ± 21.9 minutes (min) in group-1A and 79.2 ± 19.8 min in group-2A (p: 0.076). The median value of fluoroscopy time was 30 seconds (sec) in group-1A and 40 sec in group-2A (p: 0.003). In group 1A, 59 patients had no intraoperative double-J stent (DJS) insertion and 18 patients had DJS insertion, while in group 2A, 24 patients had no DJS insertion and 29 patients had DJS insertion (p: <0.001). mPNL was performed in 81.9% of patients in the supine position and 57% in the prone position. This difference was statistically significant (p:<0.001). No significant difference was found with regard to the success rate and the complications of mPNL.
Conclusions
Fluoroscopy time is shorter in the supine position. Moreover, the insertion rates of DJS are low. With increasing experience, we have found that our standard PNL (sPNL) rate is gradually decreasing and more mPNL is being performed.
Wheat leaf rust resistance conferred by the terminal region of Thinopyrum intermedium chromosome 7JsS
Authors: Yang Guotang , Zhang Na , Boshoff Willem H.P. , Li Hongwei , Li Bin , Li Zhensheng , Zheng Qi 
Published: May 2023

Abstract
Partial amphiploid Xiaoyan 78829 contains 14 Thinopyrum intermedium chromosomes and exhibits excellent resistance to fungal disease. To transfer this resistance to common wheat, a translocation line WTT26 was developed by pollen irradiation. Disease evaluation showed that WTT26 was nearly immune to 14 common races of Puccinia triticina, highly resistant to P. graminis f. sp. tritici Ug99 race PTKST at the seedling stage as well as to Blumeria graminis f. sp. tritici under field conditions at the adult plant stage. Cytogenetic identification, single nucleotide polymorphism array analysis and Th. intermedium-specific marker amplification, revealed that WTT26 carries the T4BS·7JsS translocation. Novel translocation lines with desirable yield characters and excellent leaf rust resistance was developed using irradiation. A total of 124 specific markers were developed using specific-locus amplified fragment sequencing to further enrich the marker information of chromosome 7JsS. Furthermore, a refined physical map of chromosome 7JsS that contains 74 specific markers was constructed with its dissection into six physical regions. Combining data from specific marker amplification and resistance evaluation resulted in a new leaf rust resistance locus mapped in the 0–69.29 Mb region on chromosome 7JsS. These new developed translocation lines and markers will contribute to wheat breeding for disease resistance.
Finding spatially variable ligand-receptor interactions from spatial transcriptomes
Authors: LI Shiying , WANG Ruohan , LIU Sitong , Li Shuai Cheng 
Published: May 2023

Abstract
Spatial transcriptomics has emerged as a groundbreaking tool for studying ligand-receptor interactions between cells, where such interactions can exhibit spatial variability. To identify spatially variable ligand-receptor interactions (SVIs), we present SPIDER, which constructs cell-cell interaction interfaces with minimized Dirichlet energy, models interface profiles with knowledge-graph-informed interaction signals, and identifies spatially variable signals with multiple probabilistic models. We applied SPIDER to twelve datasets from five platforms of various tissues and obtained intriguing insights. First, applying SPIDER to mouse-developing embryo data identifies distinct SVIs that potentially drive the early dorsal-ventral separation of esophageal tracheal progenitors, and applying SPIDER to lung samples suggests the proximal-distal differentiation axis of lung development. Then, from the pancreatic ductal adenocarcinomas dataset, SPIDER identified two SVI-defined clusters at the tumor boundary and an immune niche of mixed immune cells. Furthermore, on a curated breast cancer database of seventeen samples from three cancer subtypes, SPIDER identified transient SVIs along the tumor-microenvironment trajectory that are invariant or specific to cancer subtypes. Finally, in mouse and human cortex samples with manual annotations, SPIDER identified SVIs that improved spot clustering, trajectory inference, and single-cell reconstruction.
Shikonin enhances the efficacy of PD-1 blockade in colorectal cancer by inducing immunogenic cell death
Authors: Chen Jinghua , Liu Jie , Liu Xiaolin , Wang Jun , Wang Xiumei , Ye Xin , Xie Qi , Liang Jing , Li Yan 
Published: May 2023

Abstract
Background: PD-1 blockade has shown impressive clinical outcomes in colorectal cancers patients with high microsatellite instability (MSI-H). However, the majority of patients with colorectal cancer who present low microsatellite instability (MSI-L) or stable microsatellites (MSS) show little response to PD-1 blockade therapy. Here, we have demonstrated that Shikonin (SK) could induce cell death of CT26 cells via classically programmed and immunogenic pathways.
Methods and Results: SK promoted the membrane exposure of calreticulin and upregulated the expression of heat shock protein 70 (Hsp70). The upregulation of Hsp70 was dependent on ROS induced by SK and silencing of PKM2 in CT26 cells reverts ROS upregulation. Besides, SK synergizes with PD-1 blockade in CT26 xenograft mice model, with the increase of intramural DC cells and CD8+T cells. The expression of Hsp70 in tumor tissue was also increased in combinational SK plus αPD-1 therapy group.
Conclusions: Our study elucidated the potential role of ‘Shikonin-PKM2-ROS-Hsp70-ICD’ axis in the promotion of efficacy of PD-1 blockade in CRC treatments, providing a potential strategy and targets for improving the efficacy of PD-1 blockade in colorectal cancer.
Ontogeny shapes individual specialization
Authors: Hertel Anne , Albrecht Jörg , Selva Nuria , Sergiel Agnieszka , Hobson Keith , Janz David , Mulch Andreas , Kindberg Jonas , Hansen Jennifer , Frank Shane , Zedrosser Andreas , Mueller Thomas 
Published: May 2023

Abstract

Individual dietary specialization, where individuals occupy a subset of a population’s wider dietary niche, is of key importance for species’ resilience against environmental change. However, the ontogeny of individual specialization, as well as associated underlying social learning, genetic, and environmental drivers remain poorly understood. Using a multigenerational dataset of female European brown bears (Ursus arctos) followed since birth, we discerned the relative contributions of social learning, genetic predisposition, environmental forcings, and maternal effects to individual dietary specialization. Individual specialization varied from omnivorous to carnivorous diets spanning half a trophic position. The main determinants of this dietary specialization were maternal learning during rearing (13%), environmental similarity (12%), maternal effects (11%), and permanent individual effects (8%), whereas the contribution of genetic heritability was negligible. Importantly, the offspring’s trophic position closely resembled the trophic position of their mothers during the first 3-4 years after separation from the mother, but this relationship ceased with increasing time since separation. Our study reveals that social learning and maternal effects are as important for individual dietary specialization as environmental forcings. We propose a tighter integration of social effects into future studies of range expansion and habitat selection under global change that, to date, are mostly explained by environmental drivers.