bluuebunny/crossref_metadata_2025_split huggingface dataset:
A real-world analysis of 1,823 hospitalized osteoporotic fractures in Northeast China
Authors: Meng Qi , Wang Xinwei , Gao Yuzhong , Leslie William D. , Lix Lisa M. , Shi Xianbao , Kan Bo , Yang Shuman 
Published: January 2025

ContextThere are limited real-world data evidence assessing the clinical characteristics of hospitalized osteoporotic fractures in China.ObjectiveTo investigate the clinical characteristics of hospitalized major osteoporotic fractures in Northeast China.MethodsWe identified hospitalized fracture patients aged 50 and over from the First Affiliated Hospital of Jinzhou Medical University between January 1, 2018, and December 31, 2022. Major osteoporotic fractures including hip, vertebral, forearm and wrist, and humerus were diagnosed based on x-ray reports extracted from the electronic medical records (EMR). The cause of fracture, comorbidities, surgical methods, and anti-osteoporotic medications (AM) use were extracted from EMR.ResultsThe study population included 1823 fracture patients, 510 males and 1313 females. Over half of fracture patients were aged over 70 years. Hip fractures accounted for 68.4% of all fractures in males and 57.9% in females. For those with hip fractures, the most common sites were the femoral neck (62.9%) and intertrochanteric (35.3%). Most fractures were due to falls (81.0% in males and 80.2% in females). The two most common comorbidities for males and females were hyperlipemia (45.9% vs. 47.1%) and hypertension (38.2% vs. 41.6%). Only 4.7% males and 8.6% females were treated with AM.ConclusionsHip fractures, especially femoral neck fractures, accounted for the majority of osteoporotic fractures in a tertiary public hospital in Northeast China. Common comorbidities in these fracture patients were hyperlipemia and hypertension. There was a very low rate of AM use among these patients.
Potential ecological effects of wind farm location on the example of the Małopolska region in Poland
Authors: Blazy Rafał , Łabuz Rita 
Published: January 2025

Environmental challenges, such as climate change and resource depletion, are driving the search for alternative energy sources like wind energy. This study explores the ecological effects of installing wind turbines in Małopolska, Poland. The goals are to find suitable wind power locations, analyse the impact of distance-to-building criteria, and assess carbon emission reduction. The study was carried out in two stages: identification of possible sites for wind farms, taking into account the two criteria of distance from residential buildings of 700 m and 500 m, and estimation of potential carbon emission reductions. Results show optimal locations in north and south of Małopolska. Reducing the distance criteria doubles suitable areas, potentially decreasing Poland’s annual carbon emissions by 0.44%–1.03% and generating up to 1.49 TWh of wind energy, comparable to combined heat and power (CHP) plants in the region.
Facioscapulohumeral muscular dystrophy type 1 combined with becker muscular dystrophy: a family case report
Authors: Tan Menglin , Huo Huiyi , Feng Jieming , Wang Chandi , Jiang Suhua 
Published: January 2025

Facioscapulohumeral muscular dystrophy type 1 (FSHD1) and Becker muscular dystrophy (BMD) are distinct disorders caused by different genetic variations and exhibiting different inheritance patterns. The co-occurrence of both conditions within the same family is rare. In this case report, the proband was a 10 year-old boy who presented with eye and mouth orbicular muscles, shoulder and proximal upper and lower limbs weakness. Genetic testing showed that the number of D4Z4 repeat units in the sub-terminal region 4qA of chromosome 4q35 in the proband was only 4 (normal value ≥ 11) and, at the same time, a heterozygous deletion was found in exons 13–29 of DMD gene in the proband, thus the diagnosis was clinically and genetically compatible with both FSHD1 and BMD. Pedigree investigation revealed that his maternal grandmother, mother, aunt and cousin also had muscle weakness in the face, shoulders and limbs. Genetic testing confirmed that each of the four relatives had four D4Z4 repeats in the 4qA region, and all of them carried a heterozygous deletion in exons 13–29 of DMD. Based on the X-linked features of DMD/BMD, the maternal grandmother, mother, and aunt were diagnosed with FSHD1 combined with DMD deletion carriers, and the male cousin was diagnosed with FSHD1 combined with BMD. This study identifies a family with a co-occurrence of clinically overt FSHD1 and BMD, which has important reference value for the diagnosis and treatment of hereditary myopathies.
Desire for labor companionship and its associated factors among pregnant women attending antenatal care at public health facilities in Debre Berhan City: a cross-sectional study
Authors: Silesh Mulualem , Demisse Tesfanesh Lemma , Abebe Kidist Ayalew , Taye Birhan Tsegaw , Moltot Tebabere , Chekole Moges Sisay , Kasahun Fetene , Mekuriyaw Abebayehu Melesew , Kebede Tirusew Nigussie , Hailemeskel Kibret 
Published: January 2025

BackgroundAllowing women to have a companion of their choice during labor and delivery is a cost-effective strategy to enhance the quality of maternal care and promote a positive birth experience. Due to the limited studies on women's preferences for labour companionship, this study aimed to assess the desire for labour companionship and its associated factors among pregnant women attending ante-natal care at public health facilities in Debre Berhan City.MethodA facility-based cross-sectional study was conducted from August 1–30, 2022. A face-to-face questionnaire administered was used to collect data. Then, entered into Epi-Data version 4.6 and exported to SPSS version 25 for data analysis. In multivariable logistic regression analysis, variables with P &lt; 0.05 with AOR and 95% CI were considered statistically significant.ResultOf 408 participants, 68.6% [95% CI: 63.8, 73.9] of pregnant women desired to have companionship in labour. Women who were living in urban [AOR: 2.32; 95% CI: 1.336, 4.022], had secondary level of education [AOR: 0.39; 95% CI: 0.207, 0.726], being pregnant for the first time [AOR: 1.88; 95% CI: 1.197, 2.945], women who had a good knowledge towards labour companion [AOR: 2.4; 95% CI: 1.522, 3.797] were statistically significant with desire on labour companionship.ConclusionThe magnitude of desire towards labour companionship in this study area was found high. Place of residence, educational attainment, number of pregnancies (gravidity), and level of knowledge about labour companions significantly contribute to women's desire for labour companionship. Therefore, to increase the desire for labor companions; antenatal education about its benefits should emphasized, particularly in rural areas and among less-educated communities. Providing tailored support for primigravida women and underserved populations can also help to integrate labor companionship into maternal care.
Obesity, dietary interventions and microbiome alterations in the development and progression of prostate cancer
Authors: Trecarten Shaun , Liss Michael A. , Hamilton-Reeves Jill , DiGiovanni John 
Published: January 2025

Purpose of reviewThe role of the microbiome in prostate cancer is an emerging subject of research interest. Certain lifestyle factors, such as obesity and diet, can also impact the microbiome, which has been implicated in many diseases, such as heart disease and diabetes. However, this link has yet to be explored in detail in the context of prostate cancer. The purpose of this review is to explore the cross-talk between obesity, dietary interventions, and microbiome alterations in the development and progression of prostate cancer.Recent findingsMany possible mechanisms exist linking obesity and dietary interventions to microbiome alterations and prostate cancer. The gut microbiome produces metabolites that could play a role in prostate cancer oncogenesis, including short-chain fatty acids, cholesterol derivatives, and folic acid. The microbiome also plays a pivotal role in the prostate tumor microenvironment (TME), contributing to inflammation, local tissue hypoxia, and epithelial-mesenchymal transition. A bidirectional relationship exists between obesity and the microbiome, and certain diets can enact changes to the microbiome, its associated metabolites, and prostate cancer outcomes.SummaryCross-talk exists between obesity, dietary interventions, and the role of the microbiome in the development and progression of prostate cancer. To further our understanding, future human studies in prostate cancer should investigate microbiome changes and incorporate an assessment of microbiome-derived metabolites and cellular/immune changes in the TME.
Single-cell transcriptomics reveals the heterogeneity and function of mast cells in human ccRCC
Authors: Song Xiyu , Jiao Jianhua , Qin Jiayang , Zhang Wei , Qin Weijun , Ma Shuaijun 
Published: January 2025

IntroductionThe role of mast cells (MCs) in clear cell renal carcinoma (ccRCC) is unclear, and comprehensive single-cell studies of ccRCC MCs have not yet been performed.MethodsTo investigate the heterogeneity and effects of MCs in ccRCC, we studied single-cell transcriptomes from four ccRCC patients, integrating both single-cell sequencing and bulk tissue sequencing data from online sequencing databases, followed by validation via spatial transcriptomics and multiplex immunohistochemistry (mIHC).ResultsWe identified four MC signature genes (TPSB2, TPSAB1, CPA3, and HPGDS). MC density was significantly greater in ccRCC tissues than in normal tissues, but MC activation characteristics were not significantly different between ccRCC and normal tissues. Activated and resting MCs were defined as having high and low expression of MC receptors and mediators, respectively, whereas proliferating MCs had high expression of proliferation-related genes. The overall percentage of activated MCs in ccRCC tissues did not change significantly but shifted toward a more activated subpopulation (VEGFA+ MCs), with a concomitant decrease in proliferative MCs (TNF+ MCs) and resting MCs. An analysis of the ratio of TNF+/VEGFA+ MCs in tumors revealed that MCs exerted antitumor effects on ccRCC. However, VEGFA+MC was produced in large quantities in ccRCC tissues and promoted tumor angiogenesis compared with adjacent normal tissues, which aroused our concern. In addition, MC signature genes were associated with a better prognosis in the KIRC patient cohort in the TCGA database, which is consistent with our findings. Furthermore, the highest level of IL1B expression was observed in macrophages in ccRCC samples, and spatial transcriptome analysis revealed the colocalization of VEGFA+ MCs with IL1B+ macrophages at the tumor–normal interface.DiscussionIn conclusion, this study revealed increased MC density in ccRCC. Although the proportion of activated MCs was not significantly altered in ccRCC tissues compared with normal tissues, this finding highlights a shift in the MC phenotype from CTSGhighMCs to more activated VEGFA+MCs, providing a potential therapeutic target for inhibiting ccRCC progression.
Prognostic value of immunosuppression scores in patients with esophageal squamous cell carcinoma: a multicenter study
Authors: Xu Shao-jun , Luo Yun-fan , Huang Jin , Tu Jia-hua , Chen Chao , Shen Yan-ming , Sun Zhao-min , Chen Shu-chen 
Published: January 2025

IntroductionThe prognostic impact of human leukocyte antigen-E (HLA-E) expression and the proportion of natural killer (NK) cells in esophageal squamous cell carcinoma (ESCC) was investigated.MethodsThis study retrospectively evaluated 397 ESCC patients across two centers. The cumulative incidence of recurrence (CIR) and the incidence of tumor-related death (CID) were analyzed in various groups. An immunosuppression score (ISS) was developed based on HLA-E expression and NK cell proportion. Differences between groups were adjusted using inverse probability treatment weighting (IPTW). The factors influencing cancer-specific survival (CSS) and recurrence-free survival (RFS) were also examined.ResultsPatients with low HLA-E expression had significantly higher five-year CIR and CID compared to those with high expression (CIR: 20.7% vs. 45.1%, CID: 19.3% vs. 40.1%; p&lt; 0.001). Similarly, NK cell-positive patients had significantly better five-year CIR and CID than NK cell-negative patients (CIR: 16.3% vs. 59.6%, CID: 13.9% vs. 53.7%; p &lt; 0.001). The Sankey diagram indicated that the low ISS group had a lower recurrence and tumor-related mortality rate (p &lt; 0.05). After IPTW adjustment, the low ISS group showed improved five-year RFS (80.1% vs. 35.4%, p &lt; 0.001) and five-year CSS (82.3% vs. 42.5%, p &lt; 0.001) compared to the high ISS group.ConclusionsESCC with different ISS statuses represents two distinct biological subtypes, underscoring the need for personalized treatment strategies tailored to varying tumor behaviors.
Current status and perspectives of hematopoietic cell transplantation in patients with paroxysmal nocturnal hemoglobinuria
Authors: Ussowicz Marek , Przystupski Dawid , Mensah-Glanowska Patrycja , Piekarska Agnieszka 
Published: January 2025

BackgroundParoxysmal nocturnal hemoglobinuria (PNH) is a rare complement-driven acquired hemolytic anemia with specific presentations of hemoglobinuria, abdominal pain, fatigue, and thrombosis.ObjectiveTo review the current therapeutic strategies for PNH, including anti-complement therapy and allogeneic hematopoietic cell transplantation (alloHCT), focusing on the tailoring of the approach to the disease subtype.ResultsThe outcome of alloHCT varies depending on disease severity, thrombotic history, and response to prior therapies. Non-transplant PNH therapies include anti-C5 monoclonal antibodies that reduce terminal complement activation (eculizumab, ravulizumab, and crovalimab) and proximal complement pathway inhibitors such as pegcetacoplan (C3 inhibitor), iptacopan (complement factor B inhibitor), and danicopan (complement factor D inhibitor). Although complement inhibitors have revolutionized treatment, alloHCT remains the only curative therapy, particularly for patients who are refractory to medical management or have severe cytopenia. This review outlines the conditioning regimens used in alloHCT and summarizes recent studies showing that overall survival rates improve with less toxic conditioning protocols.ConclusionsAlloHCT can be used to manage PNH, particularly in patients who are resistant to or without access to complement-targeted therapies. Any potential cure offered by alloHCT must be counterbalanced by the significant procedure risks, including graft-versus-host disease and transplant-related mortality, particularly in patients with comorbidities. In the case of severe aplastic anemia with an associated PNH clone, immunoablative protocols based on anti-thymocyte globulin serotherapy with fludarabine and cyclophosphamide are recommended. The use of reduced toxicity protocols with fludarabine has been well-documented in patients with classic PNH. A treosulfan/fludarabine-based regimen is recommended; however, there is no consensus on optimal drug selection.
Absence of infection by Trichinella spp. (Nematoda: Trichinellidae) in free-living wild carnivores in Brazil
Authors: Andrade Lívia de Oliveira , Perin Patricia Parreira , Arias-Pacheco Carmen Andrea , Amorim Camilla de Souza , Lefort Fernanda , Pereira Fernanda Mara Aragão Macedo , Soares-Neto Lauro Leite , Bordignon Fernandes Antonio de Pádua , Oliveira Wilson Junior , Ichikawa Ricardo Shoiti , da Costa André Luiz Mota , Ruffino Paulo Henrique Peira , Werther Karin , Lux Hoppe Estevam Guilherme 
Published: January 2025

BackgroundNematodes of the genus Trichinella are foodborne zoonotic pathogens that are widespread globally. These parasites have two epidemiological cycles, domestic and sylvatic, with the latter having wild carnivores as the main reservoirs of the parasite. Trichinella spp. have been increasingly detected in wild carnivores in Argentina and Chile. Although the disease is absent in domestic animals in Brazil, there is serological evidence that the agent is circulating in wild boars in some areas. This study aimed to diagnose Trichinella spp. infection through artificial tissue digestion and histopathology of selected tissues of wild carnivores from São Paulo state, southeastern Brazil.MethodsTissue samples (forearm muscles, diaphragm, and tongue) from 53 wild carnivores (21 Canidae, 25 Felidae, 04 Mustelidae, 03 Procyonidae) were used, along with a retrospective study of the slide bank, considering samples from the period 2010 to 2021, totaling 89 free-living carnivores (42 Canidae, 42 Felidae, 03 Mustelidae, 02 Procyonidae).ResultsEither artificial digestion or histopathological analyses did not reveal any larvae suggestive of Trichinella spp., indicating that the nematode was not circulating within the target population.ConclusionTo date, there is no direct evidence of nematode circulation in wild carnivores in the study area.
From ocean to cage: evaluating the culture feasibility of Black-spotted croaker (Protonibea diacanthus)
Authors: Ghosh Shubhadeep , Dash Gyanaranjan , Dash Biswajit , Pradhan Rajesh Kumar , Megarajan Sekar , Behera Pralaya Ranjan , Ranjan Ritesh , Sen Swatipriyanka , Das Madhumita , Suresh Vettath Raghavan , Gopalakrishnan Achamveetil , Jena Joykrushna 
Published: January 2025

IntroductionThis study evaluates the feasibility of culturing the high-value marine fish Protonibea diacanthus in a polyculture system with Indian pompano (Trachinotus mookalee) using a marine cage culture setup. The study aims to determine growth performance, feed efficiency, and the potential of P. diacanthus as a candidate species for polyculture.MethodsWild-caught P. diacanthus were reared in six high-density polyethylene sea cages (Inner Diameter: 6 m; Outer Diameter: 7 m; Depth: 4.5 m; Area: 28.29 m²; Volume: 127.29 m³). Two experimental groups were established: Group-1 with 90 individuals (average weight: 130.15 ± 6.39 g) and Group-2 with 90 individuals (average weight: 287.80 ± 16.90 g). Each group was divided into three cages (30 fish/cage) and reared in triplicate with T. mookalee (2,500 fish/cage, average weight: 31.12 ± 1.17 g). The fish were cultured for 148 days and fed a commercial diet (40% crude protein, 10% crude lipid). Growth indices and feed efficiency indices were analyzed, and growth of the fish was modeled using von Bertalanffy Growth Function (VBGF).ResultsGrowth performance indices, including total length gain, body weight gain, and specific growth rate, were significantly higher in Group-1 (P ≤ 0.05). Feed efficiency indices (feed conversion ratio, feed efficiency ratio, and protein efficiency ratio) did not differ significantly between the groups (P &gt; 0.05). VBGF modeling estimated P. diacanthus asymptotic length (L∞) at 164.21 ± 3.58 cm, asymptotic weight (W∞) at 44,070.19 ± 2811 g, growth coefficient (K) at 0.30 ± 0.01 yr−1 and t₀ at -0.005 ± 0.02 yr. The growth performance index (ϕ’) was calculated at 1.91, surpassing values reported for wild populations. Polyculture with P. diacanthus did not significantly affect the growth or feed utilization of T. mookalee (P &gt; 0.05).Discussion and conclusionThe results indicate that P. diacanthus exhibits superior growth under marine cage culture conditions, with a high growth performance and compatibility in polyculture systems. These findings support the potential of P. diacanthus as a viable candidate for integration into commercial polyculture systems.
The risk control of rollover safety for new energy vehicles based on wire controlled chassis system
Authors: Wang Guosheng 
Published: January 2025

IntroductionTechnological innovation is the key to ensuring the safety performance of new energy vehicles, which is crucial for ensuring the safety performance of new energy vehicles.MethodsIn order to ensure the safety and dynamism of new energy vehicles, a fuzzy dynamic Markov control strategy was adopted to design a vehicle’s anti-rollover wire controlled chassis system, analyze its fuzzy failure efficiency, and combine fault tree analysis to assess the safety integrity level of the vehicle.ResultsThe results showed that the proposed control strategy achieved a wheel turning angle error of within 0.002° for new energy vehicles, indicating good vehicle performance. In the case of sharp turns, the proposed control strategy had a body roll angle of basically within 3.0°. The designed new energy vehicle reached the automotive safety integrity level D, which means that the designed system can maintain functional safety in the event of potential faults, significantly reducing the risk of vehicle accidents and ensuring passenger safety and vehicle dynamic stability.DiscussionBy reducing the risk of vehicle rollover under extreme driving conditions, vehicle handling and reliability could be improved, ensuring that new energy vehicles can operate more safely in a wider range of real-world scenarios. This provides a certain technical theory for the safety control of car rollover, which is helpful for the safety risk management of vehicles.
Epidemiology of endometriosis in Kazakhstan: a national population-based cohort analysis (2014–2019) using data from the national electronic healthcare system
Authors: Aimagambetova Gulzhanat , Sakko Yesbolat , Ukybassova Talshyn , Terzic Milan , Marat Aizada , Kamzayeva Nazira , Gusmanov Arnur , Zhakhina Gulnur , Yerdessov Sauran , Mussina Kamilla , Syssoyev Dmitriy , Gaipov Abduzhappar 
Published: January 2025

BackgroundEndometriosis is a condition affecting reproductive-age women and associated with dysmenorrhea, pelvic organs dysfunction, pelvic pain, and infertility. The real epidemiology of endometriosis remains underestimated. No data are available on prevalence of endometriosis in Kazakhstan. Therefore, the aim of this was to investigate the epidemiology, complications, surgical management approach, and outcomes of endometriosis in Kazakhstan by analyzing large-scale Kazakhstani healthcare data from the Unified Nationwide Electronic Health System (UNEHS).MethodsA population-based study among women with endometriosis treated in any healthcare setting of the Republic of Kazakhstan during the period of 2014–2019 was performed. The International Classification of Diseases (ICD) 10th edition was used to retrieve data on endometriosis (“N80” and “N97”). ICD 9th edition’s procedural codes were utilized to retrieve information on surgical procedures performed to manage patients with endometriosis.ResultsIn total, 7,682 records of women diagnosed with endometriosis were analyzed from all Kazakhstani regions. The overall prevalence of endometriosis among Kazakhstani female population was 0.12%, with 50.1% of them suffering from endometriosis of the uterus, 34.5% with ovarian endometriosis, and 9.5% with endometriosis of pelvic peritoneum. The most affected group was reproductive-age women (25–44 years old). Endometriosis rates were higher among women of 35–39, 40–44, and 45–49 years old age groups – 0.4 per 1000 women of corresponding age. The most common procedures performed for surgical management were laparoscopic cystectomy and closed biopsy of the uterus, 16.4 and 13.5%, respectively.ConclusionAmong all registered cases of endometriosis, ovarian endometriosis is the most prevalent condition. However, the analysis of the UNEHS records on endometriosis reveals incomplete and inconsistent registration of the disease, which results in the underestimation of the disease’s real burden. Clinical specialist and health authorities in Kazakhstan must work to ensure the endometriosis proper diagnosis end registration to improve the disease management and outcomes.