bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Cellular Ground Truth to Develop MRI Signatures in Glioma Models by Correlative Light Sheet Microscopy and Atlas-Based Coregistration
Authors: Schregel Katharina , Heinz Lennart , Hunger Jessica , Pan Chenchen , Bode Julia , Fischer Manuel , Sturm Volker , Venkataramani Varun , Karimian-Jazi Kianush , Agardy Dennis A. , Streibel Yannik , Zerelles Roland , Wick Wolfgang , Heiland Sabine , Bunse Theresa , Tews Björn , Platten Michael , Winkler Frank , Bendszus Martin , Breckwoldt Michael O. 
Published: July 2023

Glioblastoma is the most common malignant primary brain tumor with poor overall survival. Magnetic resonance imaging (MRI) is the main imaging modality for glioblastoma but has inherent shortcomings. The molecular and cellular basis of MR signals is incompletely understood. We established a ground truth-based image analysis platform to coregister MRI and light sheet microscopy (LSM) data to each other and to an anatomic reference atlas for quantification of 20 predefined anatomic subregions. Our pipeline also includes a segmentation and quantification approach for single myeloid cells in entire LSM datasets. This method was applied to three preclinical glioma models in male and female mice (GL261, U87MG, and S24), which exhibit different key features of the human glioma. Multiparametric MR data including T2-weighted sequences, diffusion tensor imaging, T2 and T2* relaxometry were acquired. Following tissue clearing, LSM focused on the analysis of tumor cell density, microvasculature, and innate immune cell infiltration. Correlated analysis revealed differences in quantitative MRI metrics between the tumor-bearing and the contralateral hemisphere. LSM identified tumor subregions that differed in their MRI characteristics, indicating tumor heterogeneity. Interestingly, MRI signatures, defined as unique combinations of different MRI parameters, differed greatly between the models. The direct correlation of MRI and LSM allows an in-depth characterization of preclinical glioma and can be used to decipher the structural, cellular, and, likely, molecular basis of tumoral MRI biomarkers. Our approach may be applied in other preclinical brain tumor or neurologic disease models, and the derived MRI signatures could ultimately inform image interpretation in a clinical setting.SIGNIFICANCE STATEMENTWe established a histologic ground truth-based approach for MR image analyses and tested this method in three preclinical glioma models exhibiting different features of glioblastoma. Coregistration of light sheet microscopy to MRI allowed for an evaluation of quantitative MRI data in histologically distinct tumor subregions. Coregistration to a mouse brain atlas enabled a regional comparison of MRI parameters with a histologically informed interpretation of the results. Our approach is transferable to other preclinical models of brain tumors and further neurologic disorders. The method can be used to decipher the structural, cellular, and molecular basis of MRI signal characteristics. Ultimately, information derived from such analyses could strengthen the neuroradiological evaluation of glioblastoma as they enhance the interpretation of MRI data.
PENDEKATAN GEREJA DALAM MENGHADAPI KONFLIK PERKAHWINAN SESAMA JANTINA: SATU KAJIAN KEPUSTAKAAN
Authors: Muhammad Azizan bin Sabjan  , Md Yasim Mohd Azwan 
Published: July 2023

The circulation of globalization in line with human change forms a family institution. Now, it is very shocking when the issue of same-sex marriage (PSJ) succeeds in threatening the sanctity of Christianity. This issue successfully pressured church institutions to take their own steps in dealing with homosexuals who want to hold PSJ. Thus, this library study aims to identify differences in church approaches in dealing with PSJ conflicts in Christianity. The results of the content analysis found that the church's approach to supporting PSJ was: love and affection between the sexes became bait for homophilic; declare acceptance of homosexual practitioners; the church is open to accepting homosexual practitioners to reduce anxiety; the church allowed PSJ at the urging of influential individuals; and the church relies on government funds to be pressured if it appoints pastors who oppose PSJ. Meanwhile, the church's approach against PSJ is: the central church forms believers following the teachings of the Bible; the church deals with homosexual practitioners by identifying the causes of homosexuality; the firmness of the church; church agreement; gain full authority to manage marriage; appointing church leaders from among men; and support heterosexual marriages. Therefore, it is suggested that the church take a long-term approach in order to continue to successfully maintain the sanctity of Christianity.

Peredaran arus globalisasi seiring dengan perubahan manusia membentuk institusi keluarga. Kini, amat mengejutkan apabila isu perkahwinan sesama jantina (PSJ) berjaya menggugat kesucian agama Kristian. Isu ini berjaya menekan institusi-institusi gereja untuk mengambil langkah tersendiri dalam menghadapi penganut homoseksual yang ingin melangsungkan PSJ. Justeru, kajian kepustakaan ini bertujuan untuk mengenal pasti perbezaan pendekatan gereja dalam menghadapi konflik PSJ di dalam agama Kristian. Hasil analisis kandungan mendapati pendekatan gereja menyokong PSJ adalah: cinta dan sayang sesama jantina menjadi umpan kepada homofilia; gereja bersikap terbuka menerima pengamal homoseksual untuk kurangkan kebimbangan; mengisytiharkan penerimaan pengamal homoseksual; gereja membenarkan PSJ kerana desakan individu berpengaruh; dan gereja bergantung dana kerajaan diberi tekanan jika melantik paderi yang menentang PSJ. Sementara itu, pendekatan gereja menentang PSJ pula adalah: gereja pusat membentuk penganut dahulukan Yehuwa dalam segala hal; gereja tangani pengamal homoseksual dengan mengenal pasti punca manusia melakukan seks sesama jantina; ketegasan gereja; kesepakatan gereja; mendapat kuasa penuh menguruskan perkahwinan; melantik pemimpin gereja dari kalangan lelaki; dan sokong perkahwinan heteroseksual. Justeru, dicadangkan supaya gereja mengambil pendekatan jangka masa panjang agar terus berjaya kekalkan kesucian agama Kristian.
Remoción de semillas por la ardilla Notocitellus adocetus (Rodentia: Sciuridae) en el oeste de México
Authors: Flores-Alta Daniel , Rivera-Ortiz Francisco Alberto , Contreras González Ana María 
Published: July 2023

Introducción: La dispersión y depredación de semillas tienen efectos importantes en la diversidad de plantas y estructura de las comunidades. Los roedores participan en estos tipos de interacciones. Objetivos: El objetivo de este estudio fue evaluar la remoción de semillas de Crescentia alata, Randia capitata y Zea mays por la ardilla Notocitellus adocetus para determinar su efecto en estas especies de plantas, dispersando o depredando semillas. Métodos: observamos el forrajeo y usamos cámaras trampas para determinar la parte del fruto (semilla y/o pulpa) consumida y la intensidad de consumo por las ardillas. También colocamos trampas de arena fina (FST) para medir el porcentaje de remoción de semillas. Cuantificamos los frutos producidos por las especies estudiadas y el porcentaje de daño ocasionado por N. adocetus, mediante parcelas. Resultados: Notocitellus adocetus se alimenta de las semillas y pulpa de C. alata y Z. mays. La especie que tuvo mayor tasa de remoción y mayor porcentaje de daño fue C. alata. La especie con mayor porcentaje de remoción, mayor número de frutos y menor daño en las FST fue Z. mays. En las trampas de arena fina, R. capitata tuvo la menor remoción de semillas. Conclusiones: Notocitellus adocetus es considerada depredadora de semillas, no obstante, por su comportamiento y las características de los frutos de C. alata y R. capitata, este roedor podría dejar disponibles las semillas a dispersores secundarios de semillas.
Dispersion of seeds at night and day in sub-Andean forests, Cueva Los Guacharos Park, Colombia
Authors: Bautista Plazas Sebastián , Stevenson Diaz Pablo Roberto 
Published: July 2023

Introduction: Much has been argued about the importance of the role that different types of frugivores play as seed dispersers in plant communities, but few studies have been actually carried out. Objective: We evaluated and compared diurnal and nocturnal seed dispersal in a primary and secondary sub-Andean forest in Southern Colombia. Methods: We estimated number and biomass (kg/ha) of seeds dispersed over one year (2017) using 60 fruit traps that were checked before sunrise and before sunset for 10 continuous days every two months. Results: We found that from 1 874 dispersed seeds, 55.8 % (10.9 kg/ha) were collected during the day and 44.2 % (6.39 kg/ha) at night. There were no significant differences between diurnal and nocturnal seed dispersal in number of seeds or biomass. We also found no difference in between seed dispersal that in the primary forest and secondary forest over time. Within diurnal organisms, primates and fruit-eating birds appear to be primarily responsible for this ecosystem service during the day; while bats, birds (e.g. oilbirds), and probably primates did so during the night. Conclusions: Diurnal and nocturnal dispersal seem to be equal in terms of number and weight of seeds, but not in the similarity of dispersed plant species. It remains a challenge to recognize the relative importance of different fruit-eating organisms as seed dispersers using fruit trap methods.
Severe Hypocalcemia in the Adolescent as the Only Manifestation of <i>22q11</i> Microdeletion Syndrome: Clinical Case
Authors: Vechkasova Anastasia O. , Buchinskaya Natalia V. , Kostik Mikhail M. 
Published: July 2023

Background. In this article, we would like to describe the atypical clinical picture and course of 22q11 microdeletion syndrome in a patient without specific phenotypic signs and symptoms typical for this disease.Clinical case description. Male patient, 13 years old, was hospitalized for the first time with seizure and multiple spinal fractures caused by hypocalcemia. He was referred to rheumatologist and clinical geneticist after hospital stay. Differential diagnosis included not only various bones metabolic diseases, but also 22q11 deletion syndrome. Later it was confirmed via FISH test.Conclusion. This clinical case proves once again the uniqueness of every single case, as well as the importance of comprehensive approach to the diagnosis and management of such patients.
Post-COVID Syndrome in Children: One-Time Survey Study of Parents’ Opinion
Authors: Shagieva Dilara R. , Kutlubaev Mansur A. , Rakhmatullin Airat R. 
Published: July 2023

Background. COVID-19 symptoms often persist for a long time, it indicates the post-COVID syndrome development. Its frequency in children population is generally studied by interviewing the children themselves. This approach limits the risk evaluation of post-COVID syndrome development in young children who are unable to describe the persistent symptoms due to their age.Objective. The aim of the study is to evaluate the prevalence of post-COVID syndrome in children and its effect on their daily activities by interviewing parents.Methods. The survey covered parents of children (aged from 3 months to 18 years) who suffered laboratory-confirmed COVID-19 in 2021–2022 but not earlier than 12 weeks before study initiation. The survey was conducted on-line. The presence of the symptoms (persisted or occurred 12 weeks after COVID-19), its impact on children’s daily life, the need for medical treatment or doctor's advice (due to these post-covid symptoms) and vaccination against novel coronavirus infection were evaluated. Incidence of post-COVID syndrome was analyzed in subgroups based on sex, age (< 3 years, 3–6 and 7–17 years), and disease severity.Results. Invitations to participate in the study were sent to 2292 parents of all children registered at the clinic and suffered from COVID-19 via WhatsApp and Telegram messengers. 1533 (66.9%) of them agreed to take part in the survey, and 1258 (54.8%) filled out the questionnaire in full. The survey has revealed that at least one symptom that persisted or occurred 12 weeks after COVID-19 was noted by parents in 764 out of 1258 (60.6%) children (more often in the older age group and in severe cases). Significant negative impact of symptoms on children’s daily life was noted by 251 out of 764 (32.9%) respondents. Parents of 734 out of 764 (96.1%) children have visited a doctor due to post-COVID syndrome symptoms.Conclusion. Post-COVID syndrome develops in more than 60% of children after laboratory-confirmed COVID-19, according to parents. However, we have reasons to believe that parental estimations could overestimate the prevalence of post-COVID syndrome.
Viral Encephalitis (Human Herpes Virus Type 6) after COVID-19 in a Child: Clinical Case
Authors: Zhetishev Rashid A. , Arkhestova Diana R. , Pacheva Oksana A. , Dinaeva Lidiya R. , Kamyshova Elena A. , Pazova Zhanna Yu. 
Published: July 2023

Background. Immune system disorders early after COVID-19 are associated with high risk of other infections development, including viral ones. Their diagnosis is complicated especially in the absence of clinical symptoms of primary infection and alongside with serious disease course. There are no reports on encephalitis development due to reactivation of latent viral infection.Clinical case description. Girl, 4 years-old, had psycho-neurological symptoms followed by tonic seizures on the next day. The child was hospitalized on the 3rd day after disease onset with preliminary diagnosis of viral encephalitis. The child has contacted with patients with laboratory-confirmed new coronavirus infection 3-4 weeks before the disease onset, later she had signs of mild respiratory infection (no examination of SARS-CoV-2 was carried out). Autoimmune nature of central nervous system injury was suspected after excluding a wide range of infections (negative PCR results for SARS-CoV-2, cytomegalovirus, herpes simplex virus, Epstein-Barr virus, toxoplasma, enterovirus), however it was not confirmed later on. Human herpes virus type 6 was revealed via blood tests and oropharyngeal swаb on the 15th day of disease. High concentration of IgG antibodies to SARS-CoV-2 was found was revealed as well. Treatment (antibacterial, anticonvulsant, anticoagulant, antiviral, immunosupportive, and sedative therapy, glucocorticosteroids) did not achieve significant improvement. Brain MRI (on the 23rd day of the disease) has shown leukoencephalopathy zones in subcortical white matter of convexital surfaces of parietal and occipital lobes, subatrophic changes in white matter of cerebral hemispheres with moderate vicarious enlargement of the subarachnoid spaces. Disease progression led to patient’s death on the 32nd day after first signs appearance.Conclusion. The development of severe viral encephalitis (as a result of primary herpes virus infection or its reactivation) in the early period after mild COVID-19 is shown. Its cause-effect relations require further examination.
The relationship between ghrelin and ghrelin leu72met polymorphism in coronary artery disease
Authors: YILDIRIM YAROĞLU Hatice , ÖZKAN Buğra , BALCI Senay , ÜNAL Zeynep Nil , ERDEN ERTÜRK Sema , DİLEK	ÇİÇEK Dilek	çiçek , TAMER Lülüfer 
Published: July 2023

Purpose: Ghrelin represents a hormone, which is defined as an endogenous ligand bound to the growth hormone-releasing hormone receptor (GHS-R1a). However, the mechanisms that underlie ghrelin’s impacts on cardiovascular diseases have not been completely detected. For this reason, we aimed to research the relationship between serum ghrelin and ghrelin gene polymorphism in coronary artery disease (CAD).
Materials and Methods: The study group consisted of 88 patients diagnosed with a minimum of one coronary artery stenosis over 70%, and the control group comprised 81 individuals without coronary artery lesions. An autoanalyzer was used to analyze fasting blood glucose (FBG) and lipid parameter levels. Ghrelin levels were examined with an enzyme-linked immunosorbent assay (ELISA) kit.
Results: Ghrelin levels were found to be 2.2 ng/ml in the control group and 2.1 ng/ml in the CAD group. No statistical relation in ghrelin Leu72Met genotypes were detected between the control and patient groups.
Conclusion: Serum ghrelin levels were higher in the control group than in the CAD group. Whether ghrelin levels and Leu72Met polymorphism have protective effects in CAD must be revealed in an extensive study group with other polymorphisms and ghrelin expression in the ghrelin gene.
Gene expressions and mutation analysis in schizophrenia
Authors: KALEMCİ Serdar , BOZDAĞ BAŞKAYA Huriye Gülistan , ZEYBEK Arife 
Published: July 2023

Dear Editor;
We read with interest the article titled “CYB mtDNA mutations and the expression status of genes in the PI3K/AKT/mTOR signaling pathway in patients with schizophrenia” published in Cukurova Medical Journal by Dirican et al.
Association of sleep quality with nutritional status and body mass index in adults
Authors: İÇER Mehmet Arif , GEZMEN KARADAĞ Makbule 
Published: July 2023

Purpose: Identifying the factors that affect sleep quality will help minimize potential health risks. This study aimed to evaluate the association of sleep quality with dietary habits, nutritional status and body mass index (BMI) in adults.
Materials and Methods: This study was conducted on 576 healthy individuals, 293 males and 283 females, aged between 18-65 years. The study did not include individuals with chronic diseases, pregnant and lactating women. The participants' descriptive information, dietary habits, and some anthropometric measurements were questioned. Additionally, Pittsburgh Sleep Quality Index (PSQI) was applied to the participants and 24-hour dietary recalls were recorded.
Results: In both male and total participants, the rate of good sleep quality of those with a BMI between 18.50-24.99 kg/m2 was lower than those with a BMI ≥25 kg/m2. The number of cigarette smoked in a day and the total PSQI score of the male and total participants were positively correlated. Additionally, there was a positive correlation between the percentage of saturated fatty acids in the diet and the total PSQI scores in female and total participants, while a negative relationship was found between dietary niacin (mg) and copper (mg) intakes and total PSQI scores in female.
Conclusion: The results of the study indicate that there is a relationship between sleep quality and nutritional status, and BMI. It can be concluded that some lifestyle modifications such as smoking cessation/reduction and the acquisition of healthy eating habits can be used to improve sleep quality.
Association of Kruppel-like factor 2 polymorphisms with the risk of coronary artery disease
Authors: DEĞİRMENCİ Ulaş , YILDIRIM Metin , AKKAPULU Merih , YALIN Ali , DİLEK	ÇİÇEK Dilek	çiçek , ÖZTORNACI Ragıp Onur , TAŞDELEN Bahar , YALIN Serap 
Published: July 2023

Purpose: Coronary artery disease is defined as complications that develop in proportion to the prevalence of ischemia due to occlusion of the coronary arteries and the resulting cell death. The development of atherosclerosis is significantly influenced by endothelial cell dysfunction. Kruppel-like factor 2, a transcription factor, has been shown to regulate critical biological events in endothelial biology, such as vascular tone, migration, proliferation, vasoreactivity, and angiogenesis. In our study, it was aimed to clarify the relationship between coronary artery disease and Kruppel-like factor 2 protein levels and C1239A polymorphisms.
Materials and Methods: 191 individuals who underwent coronary angiography at Mersin University Faculty of Medicine, Department of Cardiology, Health, Research and Application Center were included in the study. Measurements of serum fasting blood glucose, HDL cholesterol, total cholesterol, and triglyceride levels were performed on the AU5800 (Beckman Coulter, United States) autoanalyzer. Serum LDL levels were calculated using the Friedwald equation. Serum Kruppel-like factor 2 protein levels were measured by sandwich enzyme-linked immunoassay method on the Multiskan GO (Thermo Scientific, Finland) device. Kruppel-like factor 2 C1239A variations were detected on the Applied Biosystem VIIA™ 7 Real-Time PCR (Life Technologies Co., United States) device by TaqMan® single nucleotide polymorphism (SNP) genotyping method.
Results: Men had a 3.8-fold higher risk of CAD than women. (Odd’s ratio 3.83, 95% Confidence interval 1.98-7.39; p
QUALITY OF LIFE DURING PREGNANCY: A COMPARATIVE STUDY BETWEEN URBAN AND RURAL AREAS IN INDONESIA
Authors: Puspitasari Nunik 
Published: July 2023

Assessing quality of life during pregnancy is vital to find timely preventive measures against pregnancy complications. The quality of life during pregnancy determines pregnant women’s health. This study aims to identify the differences in the quality of life during pregnancy who participated in antenatal care (ANC) visits in primary healthcare centers in rural and urban areas. This study used a cross-sectional design and collected data through survey. The research population was 800 pregnant women who participated in ANC in three primary healthcare centers in urban areas and three others in rural areas. The quality of life during pregnancy questionnaire was used for data collection. The analysis was done using the Chi-square test and ordinal regression. Results showed no difference in the quality of life during pregnancy between women in urban areas and those in rural areas. Some factors that affected the quality of life during pregnancy included education, fetal age, number of pregnancies, number of live children, and pregnancy plans in women living in urban areas. Meanwhile, in women living in rural areas, the quality of life during pregnancy was affected by education level, occupation, fetal age, number of pregnancies, number of live children, and pregnancy plans. There are influences of age, education level, occupation, gestational age, number of pregnancies, number of living children, and pregnancy plans on the quality of life of pregnant women in rural areas.