bluuebunny/crossref_metadata_2025_split huggingface dataset:
PKL is stabilized by MMS21 to negatively regulate Arabidopsis drought tolerance through directly repressing AFL1 transcription
Authors: Jing Yexing , Yang Ziyi , Yang Ruizhen , Zhang Yunwei , Qiao Weihua , Zhou Yun , Sun Jiaqiang 
Published: May 2023

Summary

Drought stress causes substantial losses in crop production per year worldwide, threatening global food security. Identification of the genetic components underlying drought tolerance in plants is of great importance. In this study, we report that loss‐of‐function of the chromatin‐remodeling factor PICKLE (PKL), which is involved in repression of transcription, enhances drought tolerance of Arabidopsis.

At first, we find that PKL interacts with ABI5 to regulate seed germination, but PKL regulates drought tolerance independently of ABI5. Then, we find that PKL is necessary for repressing the drought‐tolerant gene AFL1, which is responsible for the drought‐tolerant phenotype of pkl mutant. Genetic complementation tests demonstrate that the Chromo domain and ATPase domain but not the PHD domain are required for the function of PKL in regulating drought tolerance. Interestingly, we find that the DNA‐binding domain (DBD) is essential for the protein stability of PKL.

Furthermore, we demonstrate that the SUMO E3 ligase MMS21 interacts with and enhances the protein stability of PKL. Genetic interaction analysis shows that MMS21 and PKL additively regulate plant drought tolerance.

Collectively, our findings uncover a MMS21‐PKL‐AFL1 module in regulating plant drought tolerance and offer insights into a novel strategy to improve crop drought tolerance.
Agency in evolution of biomolecular communication
Authors: Caetano‐Anollés Gustavo 
Published: May 2023

AbstractBiomolecular communication demands that interactions between parts of a molecular system act as scaffolds for message transmission. It also requires an organized system of signs—a communicative agency—for creating and transmitting meaning. The emergence of agency, the capacity to act in a given context and generate end‐directed behaviors, has baffled evolutionary biologists for centuries. Here, I explore its emergence with knowledge grounded in over two decades of evolutionary genomic and bioinformatic exploration. Biphasic processes of growth and diversification exist that generate hierarchy and modularity in biological systems at widely ranging time scales. Similarly, a biphasic process exists in communication that constructs a message before it can be transmitted for interpretation. Transmission dissipates matter‐energy and information and involves computation. Agency emerges when molecular machinery generates hierarchical layers of vocabularies in an entangled communication network clustered around the universal Turing machine of the ribosome. Computations canalize biological systems to perform biological functions in a dissipative quest to structure long‐lived occurrents. This occurs within the confines of a “triangle of persistence” that maximizes invariance with trade‐offs between economy, flexibility, and robustness. Thus, learning from previous historical and circumstantial experiences unifies modules in a hierarchy that expands the agency of systems.
Measuring gerontological nursing competencies among aged care nurses: Cultural adaptation and psychometric validation
Authors: Ho Mu‐Hsing , Lee Jung Jae , Joo Jee Young , Bail Kasia , Liu Megan F. , Traynor Victoria 
Published: May 2023

AbstractBackgroundExisting gerontological nursing competencies include the requisite knowledge and skills required for aged care nursing. What were not previously considered were legal and ethical aspects and access to technology, e‐health and social media.ObjectivesThis study aimed to validate an Australian gerontological nursing competencies scale and assess the factors associated with for aged care nurses in Taiwan.MethodsA methodological study design was used to validate the scale with a sample of 369 aged care nurses from aged care settings including nursing homes, long‐term care facilities and aged care wards in Taiwan. The cultural adaptation and psychometric validation were evaluated. The content validity, construct validity through exploratory factor analysis and the internal consistency of the scale were assessed.ResultsThe exploratory factor analysis extracted two levels of gerontological nursing practice, ‘essential’ and ‘enhanced’, which accounted for 80.8% of the total variance. The internal consistency, split‐half reliability and test–retest reliability were excellent. Aged care nurses with higher levels of education, aged care education as their highest degree and continued education within 6 months, and certified long‐term care education had higher gerontological nursing competencies scores than those who did not.ConclusionsThis validated gerontological nursing competencies scale is a reliable and valid tool that can be implemented in future workforce planning, research and postgraduate and undergraduate curriculum and education in Taiwan and other Mandarin‐speaking locales.Implications for practiceUsing validated gerontological nursing competencies scales to explain different levels of specialist practice is crucial for addressing negative views about aged nursing and explicitly demonstrate the possibilities for career pathways in gerontological nursing.
Percutaneous extraction of a Micra AV transcatheter pacing system due to a rare sudden battery failure after 19 months from implantation: A first experience worldwide
Authors: Santobuono Vincenzo Ezio , Basile Paolo , Carella Maria Cristina , Pomarico Francesca , Favale Stefano , Ciccone Marco Matteo , Guaricci Andrea Igoren 
Published: May 2023

AbstractIntroductionMicra AV Transcatheter Pacing System (TPS) represents an innovative second‐generation leadless pacemaker which represents an effective alternative to conventional devices in selected cases. Intrinsic malfunctions of these devices are rare, requiring sometimes their retrieval. When performed in experienced centers, this procedure is safe.Case PresentationWe describe a case of sudden battery malfunction of a Micra AV TPS, which required the extraction and the placement of a new pacing system in the right ventricle.DiscussionThis case, which has never been reported, highlights the need to a careful fluoroscopic evaluation and the usefulness of remote monitoring.
A histogram‐based sampling method for point cloud registration
Authors: Ervan Osman , Temeltas Hakan 
Published: May 2023

AbstractAccurate and efficient point cloud registration is essential in various fields, such as robotics, autonomous driving and medical imaging. The size of point clouds presents a significant challenge for existing registration methods. In this paper, a novel point cloud sampling method to improve the performance of the point cloud registration process is proposed. Instead of geometric feature preservation, which is preferred in most existing sampling methods, our approach scales every point and groups the scaled points into clusters to generate a histogram for the point cloud. The histogram is then used to identify the most significant regions of the point cloud to create the downsampled output data. Experimental results demonstrate that the proposed method improves accuracy and is robust against noise. Registration errors are reduced by up to 7% in rotation and 116% in translation. Additionally, the proposed method filtered out up to 98% of noise from the point cloud that was uniformly distributed at a rate of 25%.
Orthodox Christianity in the United States: A challenge for the study of American religion
Authors: Riccardi‐Swartz Sarah 
Published: May 2023

AbstractArguably one of the oldest forms of Christianity, with a global population of more than 260 million adherents, Orthodox Christianity is a major religious system, with networks of believers on almost every continent. However, within the study of American religion, as well as most of the social sciences and humanities (not including theology), Orthodoxy has received minimal research and interest. The broad omission of Orthodoxy from the history of American religions pushes a question to the fore: Why are some forms of Christianity at the very edge of our academic topography? This article explores existing literature on Orthodox Christianity in the United States, looking at issues between emic and etic studies, notions of Eastern Christian alterity, and the rise in new research at the intersection of contemporary social issues, Orthodox theology, and religious practice. In doing so, this article draws out how Orthodoxy provides rich American religious histories tied to global politics, immigration, and nationalism, while also prompting us to reconfigure how we study religion in the United States.
Incidence rate of osteonecrosis of jaw after cancer treated with bisphosphonates and denosumab: A systematic review and meta‑analysis
Authors: Zhang Chengrong , Shen Guoshuang , Li Huihui , Xin Yuanfang , Shi Mingqiang , Zheng Yonghui , Wang Miaozhou , Liu Zhen , Zhao Yi , Zhao Fuxing , Ren Dengfeng , Zhao Jiuda 
Published: May 2023

AbstractObjectivesThis study aimed to assess the overall incidence of osteonecrosis of the jaw (ONJ) caused by bisphosphonates and denosumab when used for controlling bone cancer metastasis or as adjuvant therapy.Subjects and MethodsA systematic search of the PubMed, Embase, and Cochrane Library databases and major meetings’ proceedings as of July 30, 2022, identified randomized controlled trials (RCTs) and observational trials that evaluated ONJ caused by denosumab or bisphosphonates. The total incidence and risk ratio (RR) for ONJ were calculated using a random‐effects model.ResultsA total of 42 003 patients with various solid tumors reported in 23 RCTs were included. The overall ONJ incidence in cancer patients receiving denosumab or bisphosphonates was 2.08% (95% CI 1.37–2.91; p < .01; I2 = 94.99%). Patients receiving denosumab had a higher ONJ incidence than those receiving bisphosphonates (RR 1.64, 95% CI 1.10–2.44; p < .05; I2 = 65.4%). Subgroup analyses showed that prostate cancer patients receiving denosumab and receiving zoledronic acid had the highest ONJ incidences, 5.0% and 3.0%, respectively. The incidence of ONJ induced by different doses was also different.ConclusionsThe incidence of ONJ caused by denosumab and bisphosphonates is low, the dose of the drug and the type of cancer have certain influence on ONJ. Therefore, clinicians should use the drug reasonably to improve the quality of life of patients.
Respiratory sinus arrhythmia, negative social interactions, and fluctuations in unmet interpersonal needs: A daily diary study
Authors: MacNeil Sasha , Renaud Johanne , Gouin Jean‐Philippe 
Published: May 2023

AbstractIntroductionThis study examined daily fluctuations in the unmet interpersonal needs of thwarted belongingness and perceived burdensomeness in response to daily negative social interactions, as well as the moderating role of respiratory sinus arrhythmia (RSA) across adolescents at lower and higher risk for suicidal ideation.MethodsFifty five adolescents with major depressive disorder (MDD, i.e., higher‐risk group) and without MDD (i.e., lower‐risk group) completed measures of resting RSA, and daily measures of negative social interactions, perceived burdensomeness, and loneliness, as a proxy for thwarted belongingness, for 10 consecutive days. Within‐person analyses examined the association between daily negative social interactions and unmet interpersonal needs, and the moderating roles of RSA and higher‐risk group status. Between‐person analyses also examined the association between RSA and unmet interpersonal needs across groups.ResultsAt the within‐person level, participants reported more unmet interpersonal needs on days when they reported more negative social interactions. At the between‐person level, higher RSA was associated with decreased loneliness in both groups, and decreased burdensomeness among the higher‐risk group.ConclusionsNegative social interactions are associated with daily unmet interpersonal needs. Higher RSA may serve as a protective factor mitigating risk for unmet interpersonal needs, particularly burdensomeness, among adolescents at higher risk for suicidal ideation.
Age‐related differences in the neuromuscular performance of fatigue‐provoking exercise under severe whole‐body hyperthermia conditions
Authors: Baranauskiene Neringa , Wang Junli , Eimantas Nerijus , Solianik Rima , Brazaitis Marius 
Published: May 2023

PurposeThe purpose of this study was to determine if aging would lead to greater decline in neuromuscular function during a fatiguing task under severe whole‐body hyperthermia conditions.MethodsTwelve young (aged 19–21 years) and 11 older (aged 65–80 years) males were enrolled in the study, which comprised a randomized control trial under a thermoneutral condition at an ambient temperature of 23°C (CON) and an experimental trial with passive lower body heating in 43°C water (HWI‐43°C). Changes in neuromuscular function and fatigability, and physical performance‐influencing factors such as psychological, thermoregulatory, neuroendocrine, and immune responses to whole‐body hyperthermia were measured.ResultsA slower increase in rectal temperature, and a lower heart rate, thermal sensation, and sweating rate were observed in older males than young males in response to HWI‐43°C trial (p < 0.05). Nevertheless, prolactin increased more in response to hyperthermia in young males, while interleukin‐6 and cortisol levels increased more in older males (p < 0.05). Peripheral dopamine levels decreased in older males and increased in young males in response to hyperthermia (p < 0.05). Surprisingly, older males demonstrated greater neuromuscular fatigability resistance and faster maximal voluntary contraction (MVC) torque recovery after a 2‐min sustained isometric MVC task under thermoneutral and severe hyperthermic conditions (p < 0.05).ConclusionNeuromuscular performance during fatigue‐provoking sustained isometric exercise under severe whole‐body hyperthermia conditions appears to decline in both age groups, but a lower relative decline in torque production for older males may relate to lower psychological and thermophysiological strain along with a diminished dopamine response and prolactin release.
Fine‐scale simulation of spatial population distribution by coupling GA–ABM and big data: A case study of Dongguan, China
Authors: Lai Zhipeng , Li Shaoying , Lin Jinyao , Li Haibo 
Published: May 2023

AbstractCapturing spatial population distribution can offer useful information for urban planning to promote reasonable population distribution and allocate urban resource. Agent‐based model (ABM) based on the modeling idea of “bottom‐up” can offer the ability to simulate the complex individual behaviors that generate spatial population distribution. Previous ABMs were unable to be extended for simulation of spatial population distribution at a fine scale due to the shortage of fine characterization of the urban environment and the calibration of agents' behavior. This study filled these gaps by proposing a genetic algorithm‐ABM (GA–ABM) for fine‐scale simulation of spatial population distribution in a manufacturing metropolis. In this model, the employment and residential choice behaviors of agents were defined by the labor economic theory and discrete selection model. Multisource geospatial big data such as enterprise points‐of‐interest big data and building footprints data were used to finely characterize the labor market and urban environment to reflect the impact of agents' employment choices on their residential decision. Furthermore, the grid‐scale population investigation big data were combined with the GA to calibrate the agents' residential decision behaviors. The proposed model was used in Dongguan, the typical manufacturing metropolis in China. As a comparison, the expert‐experience‐based method‐ABM (EEBM–ABM) was also conducted by using the same data set. Through the comparison of the results produced by these two models, it was demonstrated that the model coefficient calibrated by GA could effectively reflect the agents' residential decisions. The calibrated GA–ABM is more capable than EEBM–ABM in simulating spatial population distribution in a manufacturing metropolis. Hence, the proposed model can be used to simulate spatial population distribution in a manufacturing metropolis which helps the urban planner to conduct scientific urban planning.
Using GIS to improve public health emergency response in rural areas during the COVID‐19 crisis: A case study of South Carolina, US
Authors: Carbajales‐Dale Patricia , Annan‐Coultas Dusti , Joseph Anjali , Thompson Martie , Jafarifiroozabadi Roxana , Limber Susan P. , Holaday Bonnie , Mihandoust Sahar 
Published: May 2023

AbstractGeographic information systems (GIS) have become essential tools in the public health domain, especially when it comes to monitoring and surveillance of disease. The purpose of this article is to describe and explore the benefits of using GIS to improve public health emergency response during a global pandemic and, in particular, how to effectively optimize the allocation of public health resources in a rural setting using a data‐driven approach that considers the multifactorial demand for new COVID‐19 testing sites. Herein, the authors present their interprofessional project as an example of such efforts to inform applications for practice. The team developed a GIS‐based multicriteria decision analysis model for use by decision‐makers and public health experts in similar future planning and response scenarios. Focus is placed on rural characteristics (e.g., accessibility), vulnerable populations, and daily changing conditions (e.g., COVID‐19 daily case fluctuations) that create additional challenges for public health agencies and policymakers.
Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems
Authors: Yu Chunna , Hou Kailin , Zhang Hongshan , Liang Xueshuang , Chen Cheng , Wang Zhijing , Wu Qicong , Chen Ganlin , He Jiaxu , Bai Enhui , Li Xinfen , Du Tingrui , Wang Yifan , Wang Mingshuang , Feng Shangguo , Wang Huizhong , Shen Chenjia 
Published: May 2023

SUMMARYTaxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little is known about the precise distribution of taxoids and the transcriptional regulation of taxoid biosynthesis across Taxus stems. Here, we used MALDI‐IMS analysis to visualize the taxoid distribution across Taxus mairei stems and single‐cell RNA sequencing to generate expression profiles. A single‐cell T. mairei stem atlas was created, providing a spatial distribution pattern of Taxus stem cells. Cells were reordered using a main developmental pseudotime trajectory which provided temporal distribution patterns in Taxus stem cells. Most known taxol biosynthesis‐related genes were primarily expressed in epidermal, endodermal, and xylem parenchyma cells, which caused an uneven taxoid distribution across T. mairei stems. We developed a single‐cell strategy to screen novel transcription factors (TFs) involved in taxol biosynthesis regulation. Several TF genes, such as endodermal cell‐specific MYB47 and xylem parenchyma cell‐specific NAC2 and bHLH68, were implicated as potential regulators of taxol biosynthesis. Furthermore, an ATP‐binding cassette family transporter gene, ABCG2, was proposed as a potential taxoid transporter candidate. In summary, we generated a single‐cell Taxus stem metabolic atlas and identified molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway.
Navigating imperfect policies to donate plasma: Survey on plasma donation and a pilot plasma donation program among men who have sex with men in Canada
Authors: Vesnaver Elisabeth , Gibson Emily , Goldman Mindy , Butler‐Foster Terrie , Hill Nolan E. , Lapierre Don , MacDonagh Richard , Rubini Kyle A. , Miguel Glenndl , Rosser Andrew , MacPherson Paul , Palumbo Amelia , Randall Taylor , Osbourne‐Sorrell William , O'Brien Sheila F. , Otis Joanne , Greaves Mark , Al‐Bakri Taim Bilal , Reid Marco , Labrecque Maximilian , Germain Marc , Orvis Shane , Clapperton Andrew T. , Devine Dana , Presseau Justin 
Published: May 2023

AbstractBackgroundIn 2021, Canada implemented a pilot plasma program allowing some sexually active men who have sex with men (including but not limited to gay and bisexual men; gbMSM) to donate plasma. Changes to plasma donation policy could help address inequities in access to plasma donation and increase Canada's domestically collected plasma supply if more gbMSM donate as a result. We aimed to (1) examine views regarding plasma donation and the pilot program prior to implementation and (2) identify modifiable theory‐informed predictors of gbMSM's intention to donate plasma.MethodsWe developed, piloted, and disseminated a questionnaire informed by the Theoretical Domains Framework (TDF). We recruited gbMSM in London (ON) and Calgary (AB) to an anonymous, online cross‐sectional survey.ResultsA total of 246 gbMSM completed the survey. On scales from 1 (strongly disagree) to 5 (strongly agree), general intention to donate was high (mean = 4.24; SD = 0.94). The pilot program itself was mostly acceptable (mean = 3.71, SD = 1.16), but the intention to donate under the unique requirements of the pilot program was lower than general intention (mean = 3.58; SD = 1.26). Two domains from the theoretical domains framework (TDF) (beliefs about consequences of donating plasma and social influences) were independently associated with general intention to donate.DiscussionThe pilot plasma program as an incremental step toward more inclusive policies was mostly viewed as acceptable by the impacted communities. Historical and ongoing exclusions create unique barriers to donation. There are clear opportunities for developing theory‐informed interventions to support gbMSM to donate plasma as policies continue to become more inclusive and more become eligible to donate.