bluuebunny/crossref_metadata_2025_split huggingface dataset:
The efficacy of hemiepiphysiodesis for idiopathic knee coronal angular deformity by reconstruction plate and screw: A pilot study
Authors: Maleki Arash , Qoreishi Mohamad , Bisadi Amir , Safdari Farshad , Daei Sorkhabi Amin , Fotouhi Ali , Tahririan Mohammad A. 
Published: June 2023

AbstractBackgroundAngular deformities of the lower extremities are among the most common findings in pediatric orthopedics. Alteration of the mechanical axis in the lower extremity affects the cosmetic appearance and may lead to gait disturbances, knee discomfort, patellar maltracking with or without pain, and early joint osteoarthritis. In the current study, we aimed to investigate the efficacy of 3‐hole 3.5 mm reconstruction plates in tension‐band temporary hemiepiphysiodesis for correcting idiopathic knee coronal angular deformities.MethodsThe surgical procedure was performed using an extraperiosteal tension band plate (a 3‐hole reconstruction plate) and two 3.5 mm cortical screws to treat idiopathic knee coronal angular deformity in children. The location of the hemiepiphysiodesis was determined based on the type of angular deformity present. Postoperative follow‐ups were conducted through x‐rays to measure the medial proximal tibial angle and lateral distal femoral angle of the limbs. Statistical analysis was then performed to evaluate the efficacy of the surgical treatment based on the rate of alignment change exhibited.ResultsThe study included 14 patients (25 limbs) with genu valgum deformity who underwent temporary hemiepiphysiodesis on both the distal femur and proximal tibia, with 16 proximal tibias and 15 distal femurs being corrected. The correction rate for genu valgum was found to be 0.59° per month for both proximal tibial and distal femoral hemiepiphysiodesis. Six patients (12 limbs) were also identified with genu varum deformity, and the correction rates for proximal tibial lateral hemiepiphysiodesis and distal femoral lateral hemiepiphysiodesis were 0.85° and 0.15° per month, respectively. During a mean follow‐up duration of 11 ± 5.7 months, only one case of physeal plate closure was observed, and there were no other significant complications.ConclusionTemporary hemiepiphysiodesis with a 3‐hole R‐plate and two cortical screws takes advantage of physiological physeal growth to successfully treat idiopathic angular deformities with low complication rates.
Investigating preferences of the covid‐19 vaccine among individuals in Iran: Discrete choice experiment analysis
Authors: Parvizi Sanaz , Mehrara Mohsen , Taiebnia Ali 
Published: June 2023

AbstractBackground and AimsThis study aimed to estimate the preferences for COVID‐19 vaccines among a sample of Iranian adults and to understand the sources of preference heterogeneity.MethodsA web‐based survey was conducted from April to July 2021; out of 1747 participants, 678 completed the survey. Seven key attributes were selected, namely effectiveness, risk of severe side effects, risk of mild side effects, number of doses, duration of protection, location of manufacture, and price. Additionally, conditional logit and mixed logit models were used to analyze the data.ResultsThe results of this study indicate that vaccine effectiveness, protective duration, the risk of side effects, and price are the most important factors that influence vaccine preferences. Furthermore, we identified heterogeneity in preferences, indicating that not all individuals respond in the same way to vaccine attributes.ConclusionThe majority of Iranians prefer to get the Covid‐19 vaccine. Policymakers should consider these findings when implementing successful programs. This study contributes to the literature by estimating Iranian respondents' preferences for the Covid‐19 vaccine and identifying the heterogeneity in their preferences for vaccine attributes. The findings may also inform future research and policies related to Covid‐19 vaccination programs in Iran.
Association between immune checkpoint inhibitor‐induced myocarditis and concomitant use of thiazide diuretics
Authors: Mitsuboshi Satoru , Hamano Hirofumi , Niimura Takahiro , Ozaki Aya F. , Patel Pranav M. , Lin Tsung‐Jen , Tanaka Yuta , Kimura Ikuya , Iwata Naohiro , Shiromizu Shoya , Chuma Masayuki , Koyama Toshihiro , Yamanishi Yoshihiro , Kanda Yasunari , Ishizawa Keisuke , Zamami Yoshito 
Published: June 2023

AbstractAlthough an association has been reported between diuretics and myocarditis, it is unclear whether the risk of immune checkpoint inhibitor (ICI)‐induced myocarditis is affected by concomitant diuretics. Thus, the aim of this work was to evaluate the impact of concomitant diuretics on ICI‐induced myocarditis. This cross‐sectional study used disproportionality analysis and a pharmacovigilance database to assess the risk of myocarditis with various diuretics in patients receiving ICIs via the analysis of data entered into the VigiBase database through December 2022. Multiple logistic regression analysis was performed to identify risk factors for myocarditis in patients who received ICIs. A total of 90 611 patients who received ICIs, including 975 cases of myocarditis, were included as the eligible dataset. A disproportionality in myocarditis was observed for loop diuretic use (reporting odds ratio 1.47, 95% confidence interval [CI] 1.02‐2.04, P = .03) and thiazide use (reporting odds ratio 1.76, 95% CI 1.20‐2.50, P < .01) in patients who received ICIs. The results of the multiple logistic regression analysis showed that the use of thiazides (odds ratio 1.67, 95% CI 1.15‐2.34, P < .01) was associated with an increased risk of myocarditis in patients who received ICIs. Our findings may help to predict the risk of myocarditis in patients receiving ICIs.
Decellularized bladder as scaffold to support proliferation and functionality of insulin‐secreting pancreatic cells
Authors: Dhandapani Vignesh , Vermette Patrick 
Published: June 2023

AbstractLoss in the number or function of insulin‐producing β‐cells in pancreatic islets has been associated with diabetes mellitus. Although islet transplantation can be an alternative treatment, complications such as apoptosis, ischaemia and loss of viability have been reported. The use of decellularized organs as scaffolds in tissue engineering is of interest owing to the unique ultrastructure and composition of the extracellular matrix (ECM) believed to act on tissue regeneration. In this study, a cell culture system has been designed to study the effect of decellularized porcine bladder pieces on INS‐1 cells, a cell line secreting insulin in response to glucose stimulation. Porcine bladders were decellularized using two techniques: a detergent‐containing and a detergent‐free methods. The resulting ECMs were characterized for the removal of both cells and dsDNA. INS‐1 cells were not viable on ECM produced using detergent (i.e., sodium dodecyl sulfate). INS‐1 cells were visualized following 7 days of culture on detergent‐free decellularized bladders using a cell viability and metabolism assay (MTT) and cell proliferation quantified (CyQUANT™ NF Cell Proliferation Assay). Further, glucose‐stimulated insulin secretion and immunostaining confirmed that cells were functional in response to glucose stimulation, as well as they expressed insulin and interacted with the detergent‐free produced ECM, respectively.
Biochemical Markers of Calcium and Bone Metabolism during and after Lactation in Ugandan Women with HIV on Universal Maternal Antiretroviral Therapy
Authors: Nabwire Florence , Hamill Matthew M. , Fowler Mary Glenn , Byamugisha Josaphat , Kekitiinwa Adeodata , Prentice Ann 
Published: June 2023

ABSTRACTWe reported accentuated lactational decreases in areal bone mineral density and only partial skeletal recovery after lactation in Ugandan women with HIV (WWH) initiated on tenofovir disoproxil fumarate‐based antiretroviral therapy (TDF‐based ART) during pregnancy compared to women without HIV (REF). WWH also had higher breast milk calcium in the first months of lactation. To investigate the mechanisms, we measured bone turnover markers (bone resorption: C‐terminal telopeptide [CTX]; bone formation: procollagen type 1 N‐terminal propeptide [P1NP], bone‐specific and total alkaline phosphatase [BALP, TALP]), hormones (parathyroid hormone [PTH], intact fibroblast growth factor 23 [FGF23], 1,25‐dihydroxyvitamin D [1,25(OH)2D]), vitamin D status (25‐hydroxyvitamin D [25OHD]), and indices of mineral metabolism and renal function. Blood and urine samples collected at 36 weeks of gestation, 14 and 26 weeks of lactation, and 3–6 months after lactation were analyzed. Mean 25OHD was >50 nmol/L throughout. Both groups experienced similar biochemical changes during pregnancy and lactation to women in other settings, but within these patterns, the two groups differed significantly. Notably, WWH had higher PTH (+31%) and lower 1,25(OH)2D (−9%) and TmP/GFR (−9%) throughout, lower P1NP (−27%) and plasma phosphate (−10%) in pregnancy, higher CTX (+15%) and BALP (+19%), and lower eGFR (−4%) during and after lactation. P1NP/CTX ratio was lower in WWH than REF in pregnancy (−21%), less so in lactation (−15%), and similar after lactation. Additionally, WWH had lower plasma calcium (−5%), lower FGF23 (−16%) and fasting urinary calcium (−34%) at one or both lactation timepoints, and higher fasting urinary phosphate (+22%) at 26 weeks of lactation and after lactation. These differences resemble reported TDF effects, especially raised PTH, increased bone resorption, decreased bone formation, and decreased renal function, and may explain the observed differences in bone mineral density and breast milk calcium. Further studies are needed to determine whether HIV and TDF‐based ART have long‐term consequences for maternal bone health and offspring growth. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
An approach based on genetic algorithms and machine learning coupled for studying alloy and molecular clusters by optimizing quantum energy surfaces
Authors: Rezende Umar Lucio , De Souza Leonardo A. , Belchior Jadson C. 
Published: June 2023

AbstractA new genetic algorithm has been proposed focusing on direct ab initio potential energy surface (PES) global minima search. Besides the commonly used operators, this new approach uses an operator to: improve the initial cluster generation, classify and compare all generated clusters, and use machine learning to model the quantum PES used in parallel optimization. Part of the validation process for this methodology was done with ( for ) and (, and 75). The results are in fair agreement with the literature and led to a new global minimum for . A search has been done for the lowest energies of nanoclusters with 2–8 atoms using the DFT approach and for , using DLPNO‐CCSD(T) approach. NQGA successfully performed the MP2 optimizations for cluster. In all cases, the proposed genetic algorithm located the previously reported global minima with very efficient performance. The new proposed methodology makes it possible to optimize cluster geometries directly using high‐level ab initio methods relinquishing any bias introduced by a classical approach. Our results show that this proposed method has great potential applications due to its flexibility and efficiency in identifying global minima in the tested atomic systems.
PARP12 regulates mouse oocyte meiotic maturation
Authors: Cao Guangyi , Guo Ruirui , Chen Manqi , Sun Chuanbo , Wang Jichang 
Published: June 2023

AbstractPoly(ADP‐ribosyl)ation (PARylation) is an important post‐translational modification of proteins that involves the transfer of ADP‐ribose moieties, and plays important roles in many biological processes including DNA repair, gene expression, RNA processing, ribosome biogenesis, and protein translation. Though it is accepted that PARylation is crucial for oocyte maturation, little is known about how Mono(ADP‐ribosyl)ation (MARylation) regulates this process. Here, we report that Parp12, a mon(ADP‐ribosyl) transferase of poly(ADP‐ribosyl) polymerase (PARP) family, was highly expressed at all stages of oocytes during meiotic maturation. At germinal vesicle (GV) stage, PARP12 was mainly distributed in cytoplasm. Interestingly, PARP12 formed granular aggregation near to spindle poles during metaphase I (MI) and metaphase II (MII). PARP12 depletion results in abnormal spindle organization and chromosome misalignment in mouse oocytes. Chromosome aneuploidy frequency in PARP12 knockdown oocytes was significantly increased. Importantly, PARP12 knockdown triggers activation of spindle assembly checkpoint as shown by active BUBR1 in PARP12‐KD MI oocytes. Besides, F actin was significantly attenuated in PARP12‐KD MI oocytes which may affect the asymmetric division process. Transcriptomic analysis demonstrated that PARP12 depletion disrupts transcriptome homeostasis. Collectively, our results showed that the maternally expressed mono(ADPribosyl) transferases PARP12 was essential for oocyte meiotic maturation in mouse.
Adapter dimer contamination in sRNA‐sequencing datasets predicts sequencing failure and batch effects and hampers extracellular vesicle‐sRNA analysis
Authors: Maqueda Joaquín J. , Giovanazzi Alberta , Rocha Ana Mafalda , Rocha Sara , Silva Isabel , Saraiva Nadine , Bonito Nuno , Carvalho Joana , Maia Luis , Wauben Marca H. M. , Oliveira Carla 
Published: June 2023

AbstractSmall RNA (sRNA) profiling of Extracellular Vesicles (EVs) by Next‐Generation Sequencing (NGS) often delivers poor outcomes, independently of reagents, platforms or pipelines used, which contributes to poor reproducibility of studies. Here we analysed pre/post‐sequencing quality controls (QC) to predict issues potentially biasing biological sRNA‐sequencing results from purified human milk EVs, human and mouse EV‐enriched plasma and human paraffin‐embedded tissues. Although different RNA isolation protocols and NGS platforms were used in these experiments, all datasets had samples characterized by a marked removal of reads after pre‐processing. The extent of read loss between individual samples within a dataset did not correlate with isolated RNA quantity or sequenced base quality. Rather, cDNA electropherograms revealed the presence of a constant peak whose intensity correlated with the degree of read loss and, remarkably, with the percentage of adapter dimers, which were found to be overrepresented sequences in high read‐loss samples. The analysis through a QC pipeline, which allowed us to monitor quality parameters in a step‐by‐step manner, provided compelling evidence that adapter dimer contamination was the main factor causing batch effects. We concluded this study by summarising peer‐reviewed published workflows that perform consistently well in avoiding adapter dimer contamination towards a greater likelihood of sequencing success.
Diagnostic value of the serum lactate level as a predictor of bacteremia in patients with acute cholangitis
Authors: Oura Hirotaka , Nishino Takayoshi , Murakami Daisuke , Shima Yukiko , Shirato Miho , Sugiyama Harutoshi , Arai Makoto 
Published: June 2023

AbstractBackgroundPatients with acute cholangitis (AC) have increased mortality when associated with bacteremia. This study aimed to evaluate the predictive ability of serum lactate (Lac) for positive bacteremia in patients with acute cholangitis.MethodsIn this single‐center, retrospective study, 138 consecutive patients with AC were analyzed. Their blood samples were collected and Lac was measured.ResultsA total of 50 patients showed grade I, 50 showed grade II, and 38 showed grade III severity according to the Tokyo Guidelines 2018. Positive bacteremia was observed in 71 patients, of which 15 showed grade I, 25 showed grade II, and 31 showed grade III severity. Logistic regression analysis showed that Lac was a significant predictor of bacteremia. The area under the curve of Lac and procalcitonin (PCT) for bacteremia were 0.737 and 0.780, respectively. The optimal cutoff values for bacteremia were 17 mg/dL and 2.8 ng/mL, with sensitivity of 69.0% and 68.3%, respectively. Sensitivity of Lac and PCT for bacteremia in grade I was 58.3% and 25.0%, respectively. Three patients died from AC, all of whom were positive for bacteremia and hyperlactatemia.ConclusionLac is useful for predicting bacteremia in patients with AC.
The role of genetic testing in the prevention, diagnosis, and prognosis of sudden cardiac arrest in children
Authors: Blich Miry , Oron Hodaya , Darawsha Wisam , Suleiman Mahmoud , Gepstein Lior , Boulos Monther , Lorber Avraham , Kchoury Asaad 
Published: June 2023

AbstractBackgroundDetermining the pathogenesis of sudden cardiac arrest (SCA) in children is crucial for its management and prognosis. Our aim is to analyze the role of broad genetic testing in the prevention, diagnosis, and prognosis of SCA in Children.MethodsECG, 12‐lead holter, exercise testing, cardiac imaging, familial study, and genetic testing were used to study 29 families, in whom a child experienced SCA.ResultsAfter a thorough clinical and genetic evaluation a positive diagnosis was reached in 24/29 (83%) families. Inherited channelopathies (long QT syndrome and catecholaminergic polymorphic ventricular tachycardia) were the most prevalent 20/29 (69%) diagnosis, followed by cardiomyopathy 3/29 (10%). Broad genetic testing was positive in 17/24 (71%) cases. Using the Mann–Whitney test, we found that genetic testing (effect size = 0.625, p = 0.003), ECG (effect size = 0.61, p = 0.009), and exercise test (effect size = 0.63, p = 0.047) had the highest yield in reaching the final diagnosis. Genetic testing was the only positive test available for five (17%) families. Among 155 family members evaluated through cascade screening, 73 (47%) had a positive clinical evaluation and 64 (41%) carried a pathologic mutation. During 6 ± 4.8 years of follow‐up, 58% of the survived children experienced an arrhythmic event. Of nine family members who had an ICD implant for primary prevention, four experienced appropriate ICD shock.ConclusionsThe major causes of SCA among children are genetic etiology, and genetic testing has a high yield. Family screening has an additional role in both the diagnosis and preventing of SCA.
microRNA‐1‐3p and T‐synthase mRNA have high diagnostic efficacy on intestinal mucosal barrier dysfunction in patients with severe acute pancreatitis
Authors: Wu Wen‐Bo , Jiang Xiao‐Fei , Chen Ming‐Quan 
Published: June 2023

AbstractAcute pancreatitis (AP) is an inflammatory disorder of the pancreas that can be complicated by intestinal mucosal barrier dysfunction (SAP&IBD). The current study sought to examine the diagnostic efficacy of miR‐1‐3p and T‐synthase mRNA in SAP&IBD patients. First, SAP patients were assigned to SAP&IBD and SAP groups. Serum miR‐1‐3p expression and T‐synthase mRNA expression patterns in peripheral blood B lymphocytes were measured using RT‐qPCR. Pearson tests, ROC curve analysis, and multivariate logistic regression were used to analyze the correlation between miR‐1‐3p/T‐synthase mRNA and clinical data, their diagnostic efficiency, and independent risk factors for SAP&IBD patients, respectively. The results showed that serum miR‐1‐3p in the SAP&IBD group was elevated, and T‐synthase mRNA expression in peripheral blood B lymphocytes was diminished. Additionally, serum miR‐1‐3p expression in SAP&IBD patients was negatively correlated with T‐synthase mRNA expression, and positively correlated with their Ranson score, CRP, IL‐6, DAO, and D‐Lactate levels. Meanwhile, T‐synthase mRNA level was negatively correlated with IL‐6, DAO, and D‐Lactate levels. Both, serum miR‐1‐3p, T‐synthase mRNA, and their combination were found to exhibit diagnostic efficiency for SAP&IBD patients, and were independently associated with IBD in SAP patients. Collectively, our findings suggest that miR‐1‐3p and T‐synthase serve as independent risk factors for SAP&IBD patients and can aid the diagnosis of IBD in SAP patients.
Targeting KAT2A inhibits inflammatory macrophage activation and rheumatoid arthritis through epigenetic and metabolic reprogramming
Authors: Zhang Yunkai , Gao Ying , Ding Yingying , Jiang Yuyu , Chen Huiying , Zhan Zhenzhen , Liu Xingguang 
Published: June 2023

AbstractEpigenetic regulation of inflammatory macrophages governs inflammation initiation and resolution in the pathogenesis of rheumatoid arthritis (RA). Nevertheless, the mechanisms underlying macrophage‐mediated arthritis injuries remain largely obscure. Here, we found that increased expression of lysine acetyltransferase 2A (KAT2A) in synovial tissues was closely correlated with inflammatory joint immunopathology in both RA patients and experimental arthritis mice. Administration of MB‐3, the KAT2A‐specific chemical inhibitor, significantly ameliorated the synovitis and bone destruction in collagen‐induced arthritis model. Both pharmacological inhibition and siRNA silencing of KAT2A, not only suppressed innate stimuli‐triggered proinflammatory gene (such as Il1b and Nlrp3) transcription but also impaired NLR family pyrin domain containing 3 (NLRP3) inflammasome activation in vivo and in vitro. Mechanistically, KAT2A facilitated macrophage glycolysis reprogramming through suppressing nuclear factor‐erythroid 2‐related factor 2 (NRF2) activity as well as downstream antioxidant molecules, which supported histone 3 lysine 9 acetylation (H3K9ac) and limited NRF2‐mediated transcriptional repression of proinflammatory genes. Our study proves that acetyltransferase KAT2A licenses metabolic and epigenetic reprogramming for NLRP3 inflammasome activation in inflammatory macrophages, thereby targeting KAT2A represents a potential therapeutic approach for patients suffering from RA and relevant inflammatory diseases.
Family centered nursing practices towards women and their families in the birthing context: A qualitative systematic review
Authors: Landry Isabelle , René Caroline , Demontigny Francine 
Published: June 2023

AbstractAimSynthesize qualitative evidence examining how nurses' attitudes, beliefs, and sense of efficacy and the context surrounding birth facilitate or hinder family‐centered nursing practice.DesignThematic synthesis of qualitative studies.MethodsA literature search was conducted in CINAHL, MEDLINE, PsycINFO, SCOPUS, SCIENCE DIRECT, REPÈRES, CAIRN, and ÉRUDIT from October 2020 to June 2021. The PRISMA guidelines were followed, and studies were critically appraised using the Critical Appraisal Skills Programme checklist. Data were extracted by two independent reviewers, and Thomas and Harden's qualitative thematic synthesis method was performed for data analysis.ResultsThirteen studies were included. Three analytical themes were generated: (1) sharing power: opposing beliefs, (2) feeling a sense of efficacy in fulfilling one's role, and (3) managing a challenging work environment.Patient or Public ContributionSynthesizing nurses' experience is essential to promote implementation of favourable changes for care that is more focused on the needs of families.
The association of soluble cluster of differentiation 36 with metabolic diseases: A potential biomarker and therapeutic target
Authors: Li Yun , Chen Yaxi , Ruan Xiong Z. 
Published: June 2023

AbstractA cluster of differentiation 36 (CD36), also known as fatty acid translocase, plays an important role in developing and progressing metabolic diseases. Soluble CD36 (sCD36), a circulating form of CD36, is identified in human plasma. Current studies have demonstrated that sCD36 is an early biomarker of type 2 diabetes (T2DM) and atherosclerosis risk and may act as a key molecule in organ cross‐talks directly or indirectly. This review summarizes the cell sources, molecular structure, potential production mechanism, functions, and regulators of sCD36. We highlight the association of sCD36 with hyperlipidemia, metabolic inflammation, T2DM, cardiovascular disease, non‐alcoholic fatty liver disease, diabetic kidney disease, and obesity. These studies suggest that sCD36 could be a useful biomarker for metabolic diseases in children and a potential therapeutic target in preventing metabolic diseases.