bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zona pellucida removal modifies the expression and release of specific microRNAs in domestic cat blastocysts
Authors: Veraguas-Dávila Daniel , Caamaño Diego , Saéz-Ruiz Darling , Vásquez Yazmín , Saravia Fernando , Castro Fidel Ovidio , Rodríguez-Alvarez Lleretny 
Published: September 2023

SummaryThe in vitro culture of domestic cat embryos without the zona pellucida affects their implantation capacity. MicroRNAs (miRNAs) have an important role in embryo–maternal communication and implantation. The objective of this study was to evaluate the expression of specific miRNAs in domestic cat blastocysts cultured without the zona pellucida. Two experimental groups were done: (1) domestic cat embryos cultured with the zona pellucida (zona intact control group, ZI); and (2) cultured without the zona pellucida (zona free group, ZF). The cleavage, morula and blastocyst rates were evaluated. The blastocysts and their spent medium were used for miRNA expression analysis using RT-qPCR (miR-21, miR-24, mi25, miR-29, miR-96, miR-98, miR-103, miR-191, miR-196, miR-199, miR-130, miR-155 and miR-302). The pre-mature microRNAs (pre-miRNAs) and miRNAs were evaluated in the blastocysts and only miRNAs were evaluated in the spent medium. No differences were observed in the cleavage, morula and blastocyst rates between the ZF and ZI groups (P > 0.05). For miRNAs analysis, miR-103 and miR-191 had the most stable expression and were selected as internal controls. ZF blastocysts had a higher expression of miR-21, miR-25, miR-29 and miR-199 and a lower expression of miR-96 than their ZI counterparts (P < 0.05). Furthermore, higher levels of miR-21, miR-25 and miR-98 were detected in the spent medium of ZF blastocysts (P < 0.05). In conclusion, in vitro culture of domestic cat embryos without the zona pellucida modifies the expression of miR-21, miR-25, miR-29, miR-199 and miR-96 at the blastocyst stage and the release of miR-21, miR-25 and miR-98.
Objective and subjective evaluation of a passive low-back exoskeleton during simulated logistics tasks
Authors: Mitterlehner Lukas , Li Yasmin Xinyue , Wolf Matthias 
Published: September 2023

AbstractMusculoskeletal disorders remain the most common work-related health problem in the European Union. The most common work-related musculoskeletal disorder reported by workers is backache, especially in the logistics sector. Thus, this article aims to evaluate the effects of a commercial passive low-back exoskeleton during simulated logistics tasks. Thirty participants were recruited for this study. Typical logistics tasks were simulated in a laboratory environment. Cross-over research design was utilized to assess the effects of the exoskeleton on heart rate, trunk inclination, trunk acceleration, throughput, and perceived exertion. Also, usability and acceptance were obtained using a custom questionnaire. We found mostly non-significant differences. Effects on throughput varied widely between workplaces. Usability ratings were poor and acceptance moderate. The study suggests that a holistic evaluation and implementation approach for industrial exoskeletons is necessary. Further, prior to exoskeleton implementation, workplace adaptation might be required.
Verschwiegenes Wissen
Authors: Beisbart Claus 
Published: September 2023

Was ist zu diesem Thema bereits bekannt? In der Pflege ist viel Wissen implizit: Es wird nicht versprachlicht oder kann gar nicht versprachlicht werden. Wie wird eine neue Perspektive eingebracht? Philosophische Analysen zeigen, dass unterschiedliche Wissensformen nur bedingt sprachlich erfassbar sind. Einige Erkenntnisleistungen des Menschen werden heute mit neuronalen Netzen modelliert, die für uns intransparent sind. Was sind die Auswirkungen für die Praxis? Die gute pflegerische Praxis lässt sich nicht erschöpfend in Leitlinien formulieren. Die wissenschaftliche Ausbildung von Pflegenden sollte auch die verschwiegenen Erkenntnisfähigkeiten trainieren.
Origin of Fe‐Ca‐Metasomatism in Exhumed Mantle Rocks at the MARK Area (23°N, ODP Leg 153) and Implications on the Formation of Ultramafic‐Hosted Seafloor Massive Sulfide Deposits
Authors: Coltat R. , Andreani M. , Patten C. G. C. , Godard M. , Debret B. , Escartin J. 
Published: September 2023

AbstractAt Mid‐Ocean Ridges, hot, reduced, acidic, and metal‐rich fluids are responsible for the formation of ultramafic‐hosted seafloor massive sulfide deposits (UM‐SMSs), where mantle exhumation efficiently operates. As UM‐SMSs display great structural, mineralogical, and geochemical variabilities from site to site, a simple genetic model cannot be applied. Notably, fluid circulation and Fe‐Ca metasomatism are reported in ultramafic‐hosted hydrothermal deposits exposed in ophiolites, suggesting it might have genetic implications on the formation of mineralized systems. Similar Fe‐Ca metasomatism was reported in drilled mantle rocks at the Mid Atlantic Ridge Kane (MARK) area, offering access to the vertical dimension beneath an exhumed oceanic core complex to provide an integrative study of the nature and geometry of deep magmatic and hydrothermal processes. At MARK, mantle rocks underwent complex processes of melt‐rock and fluid‐rock interactions. Magma channeling and interactions with surrounding rocks enriched mantle silicates in Fe, Co, and Zn. There, subsequent hydrothermal alteration allowed to stabilize Fe‐rich silicates. Mineralogy and geochemistry of hydrothermal phases at MARK suggest mineral crystallization under temperatures from ∼830° down to 350°C during early mantle exhumation at a depth <6.5 km below seafloor, followed by serpentinization of the massif during progressive mantle denudation. Considering the lithological heterogeneity at (ultra)slow‐spreading ridges, metal enrichment in deep mantle rocks during melt‐rock interactions may be a widespread process. In ultramafic‐dominated environments where extensional tectonics allow fluid flows to these deep zones, fluids may leach and transport metals to the surface, accounting for metal entrapment in UM‐SMSs.
Geomagnetic Effect of the Atmospheric Acoustic Resonance Excited by Earthquakes and Volcano Eruptions
Authors: Surkov V. V. , Pilipenko V. A. , Shiokawa K. 
Published: September 2023

AbstractAn analytical model of the vertical acoustic resonance between the thermosphere and the earth's surface and its geomagnetic effect has been constructed. Resonance with a frequency of several mHz occurs when an atmospheric acoustic wave (AW) is excited by vibrations of the earth or ocean surface. The derived analytical relationships give a possibility to examine the dependence of the fundamental resonant frequency on the height of the reflecting atmospheric layer and horizontal AW number. Geomagnetic disturbances caused by the impact of AW on the ionosphere are calculated within the framework of a multi‐layered model of the ground‐atmosphere‐ionosphere system. The E‐layer of the ionosphere is treated in the approximation of a thin layer with an inclined geomagnetic field, while the topside ionosphere is assumed to consist of a cold collisionless plasma. The dependence of the spectral power of magnetic perturbations on the direction of the horizontal AW propagation is examined. The magnetic perturbation spectra are shown to have maxima at frequencies close to the acoustic resonance frequency. The spectral powers of magnetic perturbations and barometric variations measured during the Iwate‐Miyagi Nairik earthquake are in accordance with the model predictions for realistic media and AW parameters. Field‐aligned currents that arise when AWs enter the ionospheric E‐layer is shown to carry measurable electromagnetic disturbances into the geomagnetically conjugated region.
Survival with chronic myeloid leukaemia after failing milestones
Authors: Lauseker Michael , Hehlmann Rüdiger , Hochhaus Andreas , Saußele Susanne 
Published: September 2023

AbstractTherapy after failing response milestones in CML is controversial. Risks associated with comorbidities, drug toxicities or transplantation may preclude switching to another tyrosine kinase inhibitor (TKI) or other treatments. No information on long-term survival of failing patients is available. To systematically analyse survival after reaching, or not reaching, response milestones, 1342 patients from CML-study IV with newly diagnosed CML in chronic phase and regular molecular tests were studied. Landmark survival analyses were done by <0.1%, 0.1–1%, >1–10% and >10% BCR::ABL1IS at 3, 6, 12 and 24 months up to 14 years. 10- to 12-year survival of patients who failed the failure milestones (>10% BCR::ABL1IS at 6 months, >1% BCR::ABL1IS at 12 months) ranged around 80%, 10% less than in responding patients. These results suggest revision of milestones. Age (more or less than 60 years) had no major impact on survival differences, but on hazard ratios and CML-specific survival. Switching to alternative therapies, which was observed in 26.9% of the patients, did not change the main results. The data show that TKI-treated patients not reaching failure milestones still may derive benefit from continuing TKI-treatment and provide a basis for individualised decisions, if failing patients are confronted with risks of alternative treatments.
Adaptive physics-informed neural operator for coarse-grained non-equilibrium flows
Authors: Zanardi Ivan , Venturi Simone , Panesi Marco 
Published: September 2023

AbstractThis work proposes a new machine learning (ML)-based paradigm aiming to enhance the computational efficiency of non-equilibrium reacting flow simulations while ensuring compliance with the underlying physics. The framework combines dimensionality reduction and neural operators through a hierarchical and adaptive deep learning strategy to learn the solution of multi-scale coarse-grained governing equations for chemical kinetics. The proposed surrogate’s architecture is structured as a tree, with leaf nodes representing separate neural operator blocks where physics is embedded in the form of multiple soft and hard constraints. The hierarchical attribute has two advantages: (i) It allows the simplification of the training phase via transfer learning, starting from the slowest temporal scales; (ii) It accelerates the prediction step by enabling adaptivity as the surrogate’s evaluation is limited to the necessary leaf nodes based on the local degree of non-equilibrium of the gas. The model is applied to the study of chemical kinetics relevant for application to hypersonic flight, and it is tested here on pure oxygen gas mixtures. In 0-$$\textrm{D}$$
D
scenarios, the proposed ML framework can adaptively predict the dynamics of almost thirty species with a maximum relative error of 4.5% for a wide range of initial conditions. Furthermore, when employed in 1-$$\textrm{D}$$
D
shock simulations, the approach shows accuracy ranging from 1% to 4.5% and a speedup of one order of magnitude compared to conventional implicit schemes employed in an operator-splitting integration framework. Given the results presented in the paper, this work lays the foundation for constructing an efficient ML-based surrogate coupled with reactive Navier-Stokes solvers for accurately characterizing non-equilibrium phenomena in multi-dimensional computational fluid dynamics simulations.
Coixendide efficacy in combination with temozolomide in glioblastoma and transcriptome analysis of the mechanism
Authors: Zhao Zhenran , Zhang Lei , Zhang Xiaohan , Yue Yong , Liu Shengchen , Li Yanan , Ban Xiang , Zhao Cuizhu , Jin Peng 
Published: September 2023

AbstractThe purpose of this study was to explore the role of coixendide (Coix) combine with temozolomide (TMZ) in the treatment of Glioblastoma (GBM) and explore its possible mechanism. CCK-8 was used to determine the inhibitory rate of Coix group, TMZ group and drug combination group on GBM cells, and the combination index (CI) was calculated to determine whether they had synergistic effect. Then RNA was extracted from each group, transcriptome sequencing was performed, and differentially expressed genes (DEGs) were identified. The possible mechanism was analyzed by GO enrichment analysis and KEGG enrichment analysis. The CI of Coix and TMZ indicating a synergistic effect when TMZ concentration is 0.1 mg/ml and Coix concentration is 2 mg/ml. Transcriptome sequencing analysis showed that interferon (IFN) related genes were down-regulated by Coix and up-regulated by TMZ and combined drugs, however, the up-regulation induced by combined drugs was less than that of TMZ. Besides IFN related genes, cholesterol metabolism pathway were also been regulated. Coix and TMZ have synergistic effects in the treatment of GBM at certain doses. RNA-Seq results suggested that the abnormal on genetic materials caused by DNA damage induced by TMZ treatment can be sensed by IFN related genes and activates antiviral IFN signaling, causing the activation of repairing mechanism and drug resistance. Coix inhibits IFN related genes, thereby inhibits drug resistance of TMZ. In addition, the activation of ferroptosis and the regulation of DEGs in cholesterol metabolism pathway were also contributed to the synergistic effects of Coix and TMZ.
Metabolomic profiling implicates mitochondrial and immune dysfunction in disease syndromes of the critically endangered black rhinoceros (Diceros bicornis)
Authors: Corder Molly L. , Petricoin Emanuel F. , Li Yue , Cleland Timothy P. , DeCandia Alexandra L. , Alonso Aguirre A. , Pukazhenthi Budhan S. 
Published: September 2023

AbstractThe critically endangered black rhinoceros (Diceros bicornis; black rhino) experiences extinction threats from poaching in-situ. The ex-situ population, which serves as a genetic reservoir against impending extinction threats, experiences its own threats to survival related to several disease syndromes not typically observed among their wild counterparts. We performed an untargeted metabolomic analysis of serum from 30 ex-situ housed black rhinos (Eastern black rhino, EBR, n = 14 animals; Southern black rhino, SBR, n = 16 animals) and analyzed differences in metabolite profiles between subspecies, sex, and health status (healthy n = 13 vs. diseased n = 14). Of the 636 metabolites detected, several were differentially (fold change > 1.5; p < 0.05) expressed between EBR vs. SBR (40 metabolites), female vs. male (36 metabolites), and healthy vs. diseased (22 metabolites). Results suggest dysregulation of propanoate, amino acid metabolism, and bile acid biosynthesis in the subspecies and sex comparisons. Assessment of healthy versus diseased rhinos indicates involvement of arachidonic acid metabolism, bile acid biosynthesis, and the pentose phosphate pathway in animals exhibiting inflammatory disease syndromes. This study represents the first systematic characterization of the circulating serum metabolome in the black rhinoceros. Findings further implicate mitochondrial and immune dysfunction as key contributors for the diverse disease syndromes reported in ex-situ managed black rhinos.
Age-related reductions in arousal-enhanced memory are moderated by trait emotion regulation
Authors: Lee Kyoungeun , Sayre Brialisse , James Taylor A. , Duarte Audrey 
Published: September 2023

AbstractEmotional arousal is known to enhance episodic memory in young adults. However, compared to valence, little is known about how healthy aging impacts arousal-enhanced memory effects. Furthermore, while emotion regulation is believed to improve with age, it is unclear how individual differences in emotion regulation influence arousal-enhanced memory. In this large-scale online study, we investigated the impact of age and individual differences in emotion regulation on arousal-enhanced memory. During encoding, participants made arousal ratings about negative, neutral, and positive images, and we compared their subsequent memory of high and low-arousal images. We found the impact of emotional arousal on memory was reduced with age, especially for older adults who habitually suppress their emotions. Our findings show that arousal-related memory benefits are reduced with advancing age, and that individual differences in habitual usage of emotion regulation impact these age-related alterations.
A probabilistic view of protein stability, conformational specificity, and design
Authors: Stern Jacob A. , Free Tyler J. , Stern Kimberlee L. , Gardiner Spencer , Dalley Nicholas A. , Bundy Bradley C. , Price Joshua L. , Wingate David , Della Corte Dennis 
Published: September 2023

AbstractVarious approaches have used neural networks as probabilistic models for the design of protein sequences. These "inverse folding" models employ different objective functions, which come with trade-offs that have not been assessed in detail before. This study introduces probabilistic definitions of protein stability and conformational specificity and demonstrates the relationship between these chemical properties and the $$p(\text {structure}|\text {seq})$$

p
(
structure
|
seq
)

Boltzmann probability objective. This links the Boltzmann probability objective function to experimentally verifiable outcomes. We propose a novel sequence decoding algorithm, referred to as “BayesDesign”, that leverages Bayes’ Rule to maximize the $$p(\text {structure}|\text {seq})$$

p
(
structure
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seq
)

objective instead of the $$p(\text {seq}|\text {structure})$$

p
(
seq
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structure
)

objective common in inverse folding models. The efficacy of BayesDesign is evaluated in the context of two protein model systems, the NanoLuc enzyme and the WW structural motif. Both BayesDesign and the baseline ProteinMPNN algorithm increase the thermostability of NanoLuc and increase the conformational specificity of WW. The possible sources of error in the model are analyzed.
Depression and anxiety mediate the relationship between illness representations and perceived distress in patients with chronic pain
Authors: Rometsch Caroline , Teufel Martin , Skoda Eva-Maria , Schweda Adam , Cosci Fiammetta , Zipfel Stephan , Stengel Andreas , Salewski Christel 
Published: September 2023

AbstractIllness representations explain the individual’s perception and processing of health-related information. In a chronic condition such as persistent pain, illness representations might influence treatment adherence and outcome. This study aims to exploratively identify illness representations of patients with chronic pain and their association to mental disorders and subjective distress. 95 participants admitted to an inpatient university clinic were included. Validated instruments were used to assess illness representations (IPQ-R), mental health disorders (PHQ-D), and subjective distress (PSQ). Sociodemographic data and scores for the instruments were first inspected descriptively. Correlation, regression, and mediator analyses were conducted. Analyses indicated that the distributions of the IPQ-R range toward higher values. In regard to mental disorders (PHQ-D) and subjective distress (PSQ), we found several significant correlations with subscales of the IPQ-R. A regression analysis showed the IPQ-R subscales personal control, emotional representation and sex (males) to be significant predictors of subjective distress measured with the PSQ (F(11,86) = 11.55, p < .001, adjusted R2 = 0.545). Depression, anxiety, and stress syndromes (PHQ-D) significantly mediated the positive association between emotional representations (IPQ-R, predictor) and subjective distress (PSQ, outcome) with a total effect of c = .005, 95% CI [.005; .129]. Illness representations play a significant role in evaluating patients’ subjective distress and mental health. It is advised to incorporate illness representations into standard protocols for psychological interventions to comprehend their influence on targeted therapeutic strategies, particularly those tailored for pain management.
Enhancement of persistent currents and magnetic fields in a two dimensional quantum ring
Authors: Prasad Vinod , Arora Monika , Varsha  
Published: September 2023

AbstractWe present the study of the SiGe quantum ring (QR) modeled by an anharmonic axially symmetric potential with a centrifugal core in the effective mass approximation. We show how the femtosecond laser pulses (FLPs) can be used efficiently for controlling the induced current and magnetic field. We have compared the strength of induced currents and magnetic fields with and without pulsed laser which shows a substantial change. The spin-orbit interaction (SOI) and Zeeman energy show a massive impact on the generation and enhancement of these induced current and magnetic fields. These induced currents and magnetic fields have many applications in interdisciplinary areas. We have shown that the SOI presence with the FLP fields while competing with the confinement strength lowers the strength of the induced current and field.
Breastmilk from COVID-19 negative lactating mothers shows neutralizing activity against SARS-COV-2
Authors: Morniroli Daniela , Signorini Lucia , Dolci Maria , Vizzari Giulia , Ronchi Andrea , Pietrasanta Carlo , Pugni Lorenza , Mosca Fabio , Delbue Serena , Gianni Maria Lorella 
Published: September 2023

AbstractBreastmilk protects newborns from infections through specific and nonspecific compounds. This study investigated the neutralizing activity against SARS-CoV-2 of breastmilk from SARS-CoV-2 negative, unvaccinated mothers, and compared it to that from infected nursing mothers. We enrolled women after COVID-19 swab testing results upon maternity admission, and divided them into two groups: group A, COVID-19-positive mothers, and group B, negative mothers. Breastmilk was randomly sampled at 2, 7, and 20 days postpartum. We collected 19 samples for Group A and 41 for Group B. A microneutralization assay was used to determine the 50% neutralization (NT50) titre. The presence of neutralizing antibodies was also determined. Group A had 100% neutralizing samples at 2 days postpartum (T0), declining 7 days postpartum (T1) and 20 days postpartum (T2). Group B samples exhibited neutralizing activity mostly at 7 days postpartum (T1) (90%). Negative mothers' samples showed no correlation between NT50 titres and antibodies' presence, suggesting that non-specific breastmilk components may exert antiviral action against SARS-CoV-2.