bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cellular protection from H2O2toxicity by Fv-Hsp70. Protection via catalase and gamma-glutamyl cysteine synthase
Authors: Hino Chris , Chan Grace , Jordaan Gwen , Chang Sophia S , Saunders Jacquelyn T , Bashir Mohammad T , Hansen James E , Gera Joseph , Weisbart Richard H , Nishimura Robert N 
Published: February 2023

AbstractHeat shock proteins (HSPs), especially Hsp70 (HSPA1), have been associated with cellular protection from various cellular stresses including heat, hypoxia-ischemia, neurodegeneration, toxins, and trauma. Endogenous HSPs are often synthesized in direct response to these stresses but in many situations are inadequate in protecting cells. The present study addresses the transduction of Hsp70 into cells providing protection from acute oxidative stress by H2O2. The recombinant Fv-Hsp70 protein and two mutant Fv-Hsp70 proteins minus the ATPase domain, and minus the ATPase and terminal lid domains were tested at 0.5 and 1.0 uM concentrations after two different concentrations of H2O2treatment. All three recombinant proteins protected SH-SY5Y cells from acute H2O2toxicity. This data indicated that the protein binding domain was responsible for cellular protection. In addition, experiments pretreating cells with inhibitors of antioxidant proteins catalase and gamma-glutamylcysteine synthase (GGCS) before H2O2resulted in cell death despite treatment with Fv-Hsp70, implying that both enzymes were protected from acute oxidative stress after treatment with Fv-Hsp70. This study demonstrates that Fv-Hsp70 is protective in our experiments primarily by the protein-binding domain. The Hsp70 terminal lid domain was also not necessary for protection. Cellular protection was protective via the antioxidant proteins catalase and GGCS.
Identification of novel prognostic targets in coronary artery disease and related complications using bioinformatics and next generation sequencing data analysis
Authors: Vastrad Basavaraj , Vastrad Chanabasayya 
Published: February 2023

AbstractCoronary artery disease (CAD) is the most common cardiovascular disorder and the leading cause of heart-related deaths in world. Increasing molecular targets have been discovered for CAD and CAD - related complications prognosis and therapy. However, there is still an urgent need to identify novel biomarkers. Therefore, we evaluated biomarkers that might help the diagnosis and treatment of CAD and CAD - related complications. We searched next generation sequencing (NGS) dataset (GSE202625) and identified differentially expressed genes (DEGs) by comparing CAD and normal control samples using DESeq2. Gene ontology (GO) and pathway enrichment analyses of the DEGs were performed using the g:Profiler online database. The protein-protein interaction (PPI) network was plotted with IMEx interactome and visualized using Cytoscape. Module analysis of the PPI network was done using PEWCC1. MiRNA - hub gene regulatory network and TF - hub gene regulatory network analysis was performed to identify the hub genes, miRNAs and TFs. Receiver operating characteristic (ROC) curve analysis was used to predict the diagnostic effectiveness of the hub genes. A total of 118 DEGs (479 up regulated genes and 479 down regulated genes) were detected. The GO enrichment analysis indicated that the DEGs most significantly enriched in cellular response to stimulus and biosynthetic process. The REACTOME pathway enrichment analysis revealed that the DEGs were most significantly enriched in immune system and eukaryotic translation elongation. PPI network, modules, miRNA - hub gene regulatory network and TF - hub gene regulatory network analysis demonstrated that EGR1, SIRT1, STAT1, LRRK2, HIF1A, CSNK2B, RPS3, RPS2, RPS4X and HDAC11 were the hub genes. On the whole, the findings of this study enhance our understanding of the potential molecular mechanisms of CAD and CAD-related complications, and provide potential targets for further research.
Epigenomic perturbation of novelEGFRenhancers reduces the proliferative and invasive capacity of glioblastoma and increases sensitivity to temozolomide
Authors: Vincent Craig A. , Nissen Itzel , Hörnblad Andreas , Remeseiro Silvia 
Published: February 2023

ABSTRACTGlioblastoma (GB) is the most aggressive of all primary brain tumours. Patients typically rely on radiotherapy with concurrent temozolomide (TMZ) treatment and face a median survival of ∼14 months. Alterations in the Epidermal Growth Factor Receptor gene (EGFR) are common in GB tumours, but therapies targeting EGFR have not shown significant clinical efficacy. Here, we investigated the influence of theEGFRregulatory genome on GB cells, and identified novelEGFRenhancers located in an intronic region nearby the GB-associated SNP rs723527. Epigenomic perturbation of this regulatory region using CRISPR-based methods decreasesEGFRexpression and reduces the proliferative and invasive capacity of glioblastoma cells, while increasing their sensitivity to TMZ. The enhancer-perturbed GB cells also undergo a metabolic reprogramming in favour of mitochondrial respiration and present increased apoptosis. Our findings demonstrate how epigenomic perturbation ofEGFRenhancers can ameliorate the aggressiveness of glioblastoma cells and enhance the efficacy of TMZ treatment.SIGNIFICANCEOur study demonstrates how CRISPR/Cas9-based perturbation of enhancers can be used to modulate the expression of key cancer genes, which can help improve the effectiveness of existing cancer treatments and potentially the prognosis of difficult-to-treat cancers such as glioblastoma.
Thermal and alkaline pre-treatments of inoculum halt methanogenesis and enables cheese whey valorization by batch acidogenic fermentation
Authors: Giulianetti de Almeida Maria Paula , Mondini Camille , Bruant Guillaume , Tremblay Julien , Weissbrodt David G. , Mockaitis Gustavo 
Published: February 2023

AbstractCarboxylates like volatile fatty acids (VFAs) can be produced by acidogenic fermentation (AF) of dairy wastes like cheese whey, a massive residue produced at 160.67 million m3of which 42% are not valorized and impact the environment. In mixed-culture fermentations, selection pressures are needed to favor AF and halt methanogenesis. Inoculum pre-treatment was studied here as selective pressure for AF demineralized cheese whey in batch processes. Alkaline (NaOH, pH 8.0, 6 h) and thermal (90°C for 5 min, ice-bath until 23°C) pre-treatments, were tested together with batch operations run at initial pH 7.0 and 9.0, food-to-microorganism (F/M) ratios of 0.5 to 4.0 g COD g-1VS, and under pressurized and non-pressurized headspace, in experiments duplicated in two institutes. Acetic acid was highly produced (1.36 and 1.40 g CODAcOHL-1) at the expense of methanogenesis by combining a thermal pre-treatment of inoculum with a non-pressurized batch operation started at pH 9.0. Microbial communities comprised of VFAs and alcohol producers, such asClostridium,Fonticella, andIntestinimonas, and fermenters such asLongilineaandLeptolinea. Communities also presented the lipid-accumulating and bulk and foamingCandidatus Microthrixand the metanogenicMethanosaetaregardless of no methane production. An F/M ratio of 0.5 g COD g-1VS led to the best VFA production of 1,769.38 mg L-1. Overall, inoculum thermal pre-treatment, initial pH 9.0, and non-pressurized headspace acted as a selective pressure for halting methanogen and producing VFAs, valorizing cheese whey via batch acidogenic fermentation.
Early threat perception is independent of later cognitive and behavioral control. A virtual reality-EEG-ECG study
Authors: Lu Juanzhi , Kemmerer Selma K. , Riecke Lars , de Gelder Beatrice 
Published: February 2023

AbstractResearch on social threat has shown influences of various factors, such as agent characteristics, proximity and social interaction on social threat perception. An important, yet understudied aspect of threat experience concerns the ability to exert control over the thread. In this study, we used a Virtual Reality (VR) environment showing an approaching avatar that was either angry (threatening body expression) or neutral (neutral body expression) and informed participants to stop avatars from coming closer under five levels of control success (0, 25, 50, 75, or 100%) when they felt uncomfortable. Behavioral results revealed that social threat triggered faster reactions at a greater virtual distance from the participant than the neutral avatar. Event-related potentials (ERPs) revealed that the angry avatar elicited a larger N170/vertex positive potential (VPP) and a smaller N3 than the neutral avatar. The 100% control condition elicited a larger late positive potential (LPP) than the 75% control condition. In addition, we observed enhanced theta power and accelerated heart rate for the angry avatar vs. neutral avatar, suggesting that these measures index threat perception. Our results indicate that perception of social threat takes place in early to middle cortical processing stages, and control ability is associated with cognitive evaluation in middle to late stages.
CXCR2 expression during melanoma tumorigenesis controls transcriptional programs that facilitate tumor growth
Authors: Yang J , Bergdorf K , Yan C , Luo W , Chen SC , Ayers D , Liu Q , Liu X , Boothby M , Groves SM , Oleskie AN , Zhang X , Maeda DY , Zebala JA , Quaranta V , Richmond A 
Published: February 2023

AbstractBackgroundThough the CXCR2 chemokine receptor is known to play a key role in cancer growth and response to therapy, a direct link between expression of CXCR2 in tumor progenitor cells during induction of tumorigenesis has not been established.MethodsTo characterize the role of CXCR2 during melanoma tumorigenesis, we generated tamoxifen-inducible tyrosinase-promoter drivenBrafV600E/Pten-/-/Cxcr2-/-andNRasQ61R/INK4a-/-/Cxcr2-/-melanoma models. In addition, the effects of a CXCR1/CXCR2 antagonist, SX-682, on melanoma tumorigenesis were evaluated inBrafV600E/Pten-/-andNRasQ61R/INK4a-/-mice and in melanoma cell lines. Potential mechanisms by whichCxcr2affects melanoma tumorigenesis in these murine models were explored using RNAseq, mMCP-counter, ChIPseq, and qRT-PCR; flow cytometry, and reverse phosphoprotein analysis (RPPA).ResultsGenetic loss ofCxcr2or pharmacological inhibition of CXCR1/CXCR2 during melanoma tumor induction resulted in key changes in gene expression that reduced tumor incidence/growth and increased anti-tumor immunity. Interestingly, afterCxcr2ablation,Tfcp2l1, a key tumor suppressive transcription factor, was the only gene significantly induced with a log2fold-change greater than 2 in these three different melanoma models.ConclusionsHere, we provide novel mechanistic insight revealing how loss ofCxcr2expression/activity in melanoma tumor progenitor cells results in reduced tumor burden and creation of an anti-tumor immune microenvironment. This mechanism entails an increase in expression of the tumor suppressive transcription factor,Tfcp2l1,along with alteration in the expression of genes involved in growth regulation, tumor suppression, stemness, differentiation, and immune modulation. These gene expression changes are coincident with reduction in the activation of key growth regulatory pathways, including AKT and mTOR.
Enforced dimerization of CD45 by the adenovirus E3/49K protein inhibits T cell receptor signaling
Authors: Windheim Mark , Reubold Thomas F. , Gaestel Matthias , Burgert Hans-Gerhard 
Published: February 2023

AbstractHuman adenoviruses (HAdVs) are widespread pathogens that generally cause mild infections in immunocompetent individuals, but severe or even fatal diseases in immunocompromised patients. In order to counteract the host immune defenses HAdVs encode various immunomodulatory proteins in the early transcription unit 3 (E3). The E3/49K protein is a highly glycosylated type I transmembrane protein uniquely expressed by species D adenoviruses. Its N-terminal ectodomain sec49K is released by metalloprotease-mediated shedding at the cell surface and binds to the receptor-like protein tyrosine phosphatase CD45, a critical regulator of leukocyte activation and functions. It remained elusive which domains of CD45 and E3/49K are involved in the interaction and whether such an interaction can also occur on the cell surface with membrane-anchored full-length E3/49K. Here, we show that the two extracellular domains R1 and R2 of E3/49K bind to the same site in the domain d3 of CD45. This interaction enforces the dimerization of CD45 causing the inhibition of T cell receptor signaling. Intriguingly, the membrane-anchored E3/49K appears to be designed like a “molecular fishing rod” using an extended disordered region of E3/49K as a “fishing line” to bridge the distance between the plasma membrane of infected cells and the CD45 binding site on T cells to effectively position the domains R1 and R2 as baits for CD45 binding. This design strongly suggests that both the secreted sec49K and the membrane-anchored full-length E3/49K have immunomodulatory functions. The forced dimerization of CD45 may be applied as a therapeutic strategy in chronic inflammatory disorders and cancer.
Intracellular interaction of FNDC1/FN1/AR/cMYC in prostate cancer cells
Authors: Ghosh Priyanka , Ogunwobi Olorunseun O. 
Published: February 2023

AbstractMicroRNAs play an important role in modulating normal cellular functions through protein-protein interaction in addition to regulating gene expression via mRNA degradation or translational repression.PVT1is a non-protein coding gene that encodes six annotated microRNAs (miRNAs), including miR-1207-3p, a demonstrated modulator in PCa. MiR-1207-3p is significantly underexpressed in PCa cells when compared to normal prostatic cells and directly targets fibronectin type III domain containing 1 (FNDC1), which was found to be overexpressed in PCa cells along with concomitant overexpression of fibronectin (FN1)/androgen receptor (AR)/c-MYC. To better understand the role of FNDC1/FN1/AR/c-MYC in PCa progression we examined the interaction between FNDC1/FN1/AR/c-MYC. Coimmunoprecipitation study showed direct physical interaction between FNDC1/FN1/AR/c-MYC in PCa cells and FN1/AR/cMYC in normal prostatic cell. Knockdown of FN1, AR and cMYC not only confirmed FN1/FNDC1/AR/cMYC interaction but also gave rise to the possibility of a complex. We also examined the spatial localization of the FNDC1/FN1/AR/c-MYC pathway by performing immunofluorescence staining in PCa cells. Single staining analysis revealed that FNDC1, AR and c-MYC localize to the nucleus and cytoplasm while FN1 localizes only to the cytoplasm in PCa cells but not in the non-tumorigenic prostate cells. However, our findings show that miR-1207-3p has no direct effect on the FNDC1/FN1/AR/cMYC interaction. Understanding this molecular interaction can reveal additional insights into the role of FNDC1/FN1/AR/cMYC interaction in PCa progression.
Examining the effect of different knowledge aspects on information security awareness
Authors: Chua Hui Na , Khor Vi Vien , Wong Siew Fan 
Published: February 2023

Purpose
The purpose of this paper is to identify the different aspects of knowledge and how they associate with information security awareness (ISA). The paper also explores how ISA differs based on demographic characteristics.

Design/methodology/approach
Survey data was collected from 609 respondents in Malaysia.

Findings
The results show that increasing access to informal, multimedia learning mediums, declarative, schematic and strategic knowledge positively impacts an individual's ISA, whereas textual learning medium decreases the ISA. Respondents with different education levels significantly prefer different types of knowledge. Males learn better for ISA with schematic and strategic knowledge compared to females.

Practical implications
The research provides implications for governments and organizations in designing effective ISA campaigns.

Originality/value
Studies show that ISA is crucial in improving information systems policy compliance behavior. The literature has examined various topics ranging from the factors influencing the ISA to how ISA impacts information security behavior. However, there is a lack of study on how different aspects of knowledge impact ISA. This study identified various knowledge aspects from the literature and grouped them into the source, type of knowledge, emotion toward knowledge and learning medium.
A hybrid approach to enhancing the performance of manufacturing organizations by optimal sequencing of value stream mapping tools
Authors: Kumar Sameer , Marawar Yogesh , Soni Gunjan , Jain Vipul , Gurumurthy Anand , Kodali Rambabu 
Published: February 2023

Purpose
Lean manufacturing (LM) is prevalent in the manufacturing industry; thus, focusing on fast and accurate lean tool implementation is the new paradigm in manufacturing. Value stream mapping (VSM) is one of the many LM tools. It is understood that combining LM implementation with VSM tools can generate better outcomes. This paper aims to develop an expert system for optimal sequencing of VSM tools for lean implementation.

Design/methodology/approach
A proposed artificial neural network (ANN) model is based on the analytic network process (ANP) devised for this study. It will facilitate the selection of VSM tools in an optimal sequence.

Findings
Considering different types of wastes and their level of occurrence, organizations need a set of specific tools that will be effective in the elimination of these wastes. The developed ANP model computes a level of interrelation between wastes and VSM tools. The ANN is designed and trained by data obtained from numerous case studies, so it can predict the accurate sequence of VSM tools for any new case data set.

Originality/value
The design and use of the ANN model provide an integrated result of both empirical and practical cases, which is more accurate because all viable aspects are then considered. The proposed modeling approach is validated through implementation in an automobile manufacturing company. It has resulted in benefits, namely, reduction in bias, time required, effort required and complexity of the decision process. More importantly, according to all performance criteria and subcriteria, the main goal of this research was satisfied by increasing the accuracy of selecting the appropriate VSM tools and their optimal sequence for lean implementation.
The effect of supplier sustainability risk management strategies on supply chain performance
Authors: Silva C.W. Chathurani , Rathnayaka Dilini Dineshika , Fernando M.A.C.S. Sampath 
Published: February 2023

Purpose
This study aims to evaluate the adoption of four types of supplier sustainability risk management (SSRM) strategies, namely, risk avoidance (RA), risk acceptance (RAC), collaboration-based risk mitigation (CBM) and monitoring-based risk mitigation (MBM) in Sri Lankan apparel and retail industries, and to investigate their effect on supply chain performance (SCP).

Design/methodology/approach
This study uses the dynamic capability view (DCV) to develop its hypotheses. Data collected from 89 firms were analysed using partial least square (PLS) structural equation modelling and PLS-based multiple group analysis.

Findings
Sri Lankan apparel and retail firms adopt RA and MBM strategies relatively more than CBM and RAC strategies, whereas there is no significant difference between the two industries in terms of the use of SSRM strategies. The path analysis revealed significant effects of RA and RAC strategies on SCP of both industries. The effect of CBM strategy on SCP is moderated by industry, while MBM has no significant impact.

Research limitations/implications
While managing supplier sustainability risks effectively, RA and RAC strategies provide more opportunities for managers to improve SCP. In achieving SCP, CBM strategies are proven to be more effective for retail industry compared with the apparel sector. Although MBM strategies offer sustainability advantages to firms, their contribution to improving the performance of apparel and retail supply chains is not significant. This research is limited to only two industries (apparel and retail) in Sri Lanka, where the evidence for the effects of SSRM strategies is not available for other contexts.

Originality/value
Either the effects of the four types of SSRM strategies on SCP or the moderating effect of industry on these effects have not been empirically confirmed in the literature. Evaluating the extent to which different strategies are implemented in Sri Lankan apparel and retail industries is another significant contribution of this research. Furthermore, this study contributes by using DCV to a sustainability-based supply chain risk management research.
A case of complete Melkersson‐Rosenthal syndrome: Possibly associated with Mycolicibacterium fortuitum from odontogenic origin
Authors: Zhang Wenqing , Yan Zhimin 
Published: February 2023

AbstractMelkersson‐Rosenthal syndrome (MRS) is a neuromucocutaneous disease of unknown pathogenesis. With this communication, we describe a case of a 26‐year‐old woman with complete MRS in whom Mycolicibacterium fortuitum was detected in the swelling lip biopsy by next‐ generation sequencing. The patient's symptoms were slightly improved after intralesional corticosteroid injection combined with broad‐spectrum antibiotics, while they were significantly improved after further treatment of dental caries and removal of the residual root. This case provides insight into the possible microbial infection pathogenesis of MRS, and M. fortuitum was speculated to be related to granulomatous and neuronal disorders, most probably from odontogenic origin.
Spatial assembly of grassland communities and interrelationships with productivity
Authors: Cheng Changjin , He Nianpeng , Li Mingxu , Xu Li , Sun Osbert Jianxin 
Published: February 2023

Abstract

Knowledge on the fine‐scale spatial aggregation is pertinent for deciphering plant community dynamics, as interactions among sessile plants mainly occur in the neighbourhood. However, the spatial assemblage mechanism and interrelationships with productivity remain poorly understood.

Here, species segregation index (Mscom) was used to quantify the interspecific segregation (or intraspecific aggregation) of the neighbourhood in plant communities from three contrasting plateaus. We employed the coordinates for 125,726 individual plants (within 1 m2 plots) sampled in natural grassland communities across two temperate grasslands (Loess Plateau and Mongolian Plateau) and one alpine grassland (Tibetan Plateau) to elucidate the assembly mechanisms of Mscom and interrelationships with above‐ground net primary productivity (ANPP).

We found that these grassland communities tended to assemble into intraspecific aggregations; the phenomenon was more apparent in regions with low annual precipitation. In grassland communities on Loess Plateau, the strong intraspecific aggregation was mainly due to low sexual reproduction allocation; on Mongolian Plateau, reproductive allocation was also the dominant factor for Mscom variation, followed by the interactive effect of annual precipitation and species richness, whereas on Tibetan Plateau, low species richness was the main reason for increased intraspecific aggregation, followed by the interactive effect of annual precipitation and species richness, and reproductive allocation. More importantly, on all three plateaus, the independent influence of Mscom on ANPP is stronger than that of species and phylogenetic diversity.

We elucidated the potential mechanisms shaping spatial aggregation in grassland communities at large scale, and emphasize the critical role of spatial aggregation in predicting the variation of ANPP.

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