bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chiropterans are a hotspot for horizontal transfer of DNA transposons in Mammalia
Authors: Paulat Nicole S , Storer Jessica M , Moreno-Santillán Diana D , Osmanski Austin B , Sullivan Kevin AM , Grimshaw Jenna R , Korstian Jennifer , Halsey Michaela , Garcia Carlos J , Crookshanks Claudia , Roberts Jaquelyn , Smit Arian FA , Hubley Robert , Rosen Jeb , Teeling Emma C , Vernes Sonja C , Myers Eugene , Pippel Martin , Brown Thomas , Hiller Michael ,   Zoonomia Consortium, Rojas Danny , Dávalos Liliana M , Lindblad-Toh Kerstin , Karlsson Elinor K , Ray David A 
Published: March 2023

AbstractHorizontal transfer of transposable elements is an important mechanism contributing to genetic diversity and innovation. Bats (order Chiroptera) have repeatedly been shown to experience horizontal transfer of transposable elements at what appears to be a high rate compared to other mammals. We investigated the occurrence of horizontally transferred DNA transposons involving bats. We found over 200 putative horizontally transferred elements within bats; sixteen transposons were shared across distantly related mammalian clades and two other elements were shared with a fish and two lizard species. Our results indicate that bats are a hotspot for horizontal transfer of DNA transposons. These events broadly coincide with the diversification of several bat clades, supporting the hypothesis that DNA transposon invasions have contributed to genetic diversification of bats.
Surfactant protein A promotes atherosclerosis through mediating macrophage foam cell formation
Authors: King Skylar D. , Cai Dunpeng , Fraunfelder Mikayla M. , Chen Shi-You 
Published: March 2023

ABSTRACTBACKGROUNDAtherosclerosis is a progressive inflammatory disease where macrophage foam cells play a central role in the pathogenesis. Surfactant protein A (SPA) is a lipid-associating protein involved with regulating macrophage function in various inflammatory diseases. However, the role of SPA in atherosclerosis and macrophage foam cell formation has not been investigated.METHODSPrimary resident peritoneal macrophages were extracted from wildtype (WT) and SPA deficient (SPA-/-) mice to determine the functional effects of SPA in macrophage foam cell formation. SPA expression was assessed in healthy vessels and atherosclerotic aortic tissue from the human coronary artery and WT or apolipoprotein e-deficient (ApoE-/-) mice brachiocephalic arteries fed high fat diets (HFD) for 4 weeks. Hypercholesteremic WT and SPA-/-mice fed a HFD for 6 weeks were investigated for atherosclerotic lesionsin vivo.RESULTSIn vitroexperiments revealed that global SPA deficiency reduced intracellular cholesterol accumulation and macrophage foam cell formation. Mechanistically, SPA-/-dramatically decreased CD36 cellular and mRNA expression. SPA expression was increased in atherosclerotic lesions in humans and ApoE-/-mice.In vivoSPA deficiency attenuated atherosclerosis and reduced the number of lesion-associated macrophage foam cells.CONCLUSIONSOur results elucidate that SPA is a novel factor for atherosclerosis development. SPA enhances macrophage foam cell formation and atherosclerosis through increasing scavenger receptor cluster of differentiation antigen 36 (CD36) expression.
Meningeal origins and dynamics of perivascular fibroblast development on the mouse cerebral vasculature
Authors: Jones Hannah E. , Coelho-Santos Vanessa , Bonney Stephanie K. , Abrams Kelsey A. , Shih Andy Y. , Siegenthaler Julie A. 
Published: March 2023

AbstractPerivascular fibroblasts (PVFs) are a fibroblast-like cell type that reside on large-diameter blood vessels in the adult meninges and central nervous system (CNS). PVFs drive fibrosis following injury but their homeostatic functions are not well detailed. In mice, PVFs were previously shown to be absent from most brain regions at birth and are only detected postnatally within the cerebral cortex. However, the origin, timing, and cellular mechanisms of PVF development are not known. We usedCol1a1-GFPandCol1a2-CreERTtransgenic mice to track PVF developmental timing and progression in postnatal mice. Using a combination of lineage tracing andin vivoimaging we show that brain PVFs originate from the meninges and are first seen on parenchymal cerebrovasculature at postnatal day (P)5. After P5, PVF coverage of the cerebrovasculature rapidly expands via mechanisms of local cell proliferation and migration from the meninges, reaching adult levels at P14. Finally, we show that PVFs and perivascular macrophages (PVMs) develop concurrently along postnatal cerebral blood vessels, where the location and depth of PVMs and PVFs highly correlate. These findings provide the first complete timeline for PVF development in the brain, enabling future work into how PVF development is coordinated with cell types and structures in and around the perivascular spaces to support normal CNS vascular function.SummaryBrain perivascular fibroblasts migrate from their origin in the meninges and proliferate locally to fully cover penetrating vessels during postnatal mouse development.
Coordination of Alternative Splicing and Alternative Polyadenylation revealed by Targeted Long-Read Sequencing
Authors: Zhang Zhiping , Bae Bongmin , Cuddleston Winston H. , Miura Pedro 
Published: March 2023

AbstractNervous system development is associated with extensive regulation of alternative splicing (AS) and alternative polyadenylation (APA). AS and APA have been extensively studied in isolation, but little is known about how these processes are coordinated. Here, the coordination of cassette exon (CE) splicing and APA inDrosophilawas investigated using a targeted long-read sequencing approach we call Pull-a-Long-Seq (PL-Seq). This cost-effective method uses cDNA pulldown and Nanopore sequencing combined with an analysis pipeline to resolve the connectivity of alternative exons to alternative 3’ ends. Using PL-Seq, we identified genes that exhibit significant differences in CE splicing depending on connectivity to short versus long 3’UTRs. Genomic long 3’UTR deletion was found to alter upstream CE splicing in short 3’UTR isoforms and ELAV loss differentially affected CE splicing depending on connectivity to alternative 3’UTRs. This work highlights the importance of considering connectivity to alternative 3’UTRs when monitoring AS events.
Inoviridaeprophage and bacterial host dynamics during diversification, succession and Atlantic invasion of Pacific-nativeVibrio parahaemolyticus
Authors: Foxall Randi L. , Means Jillian , Marcinkiewicz Ashley L. , Schillaci Christopher , DeRosia-Banick Kristin , Xu Feng , Hall Jeffrey A. , Jones Stephen H. , Cooper Vaughn S. , Whistler Cheryl A. 
Published: March 2023

AbstractThe epidemiology ofVibrio parahaemolyticus,the leading cause of seafood-borne bacterial gastroenteritis of humans world-wide, dramatically changed in the United States following the establishment of a Pacific native lineage called sequence type (ST) 36 in the Atlantic. In this study we used phylogeography based on traceback to environmental source locations and comparative genomics to identify features that promoted evolution, dispersal, and competitive dominance of ST36. The major genomic differentiation and competitive success of ST36 was associated with a striking succession of filamentous prophage in the familyInoviridae(inoviruses), including loss of an inovirus prophage that had been maintained for decades in the endemic north Pacific population. Subsequently, at least five distinct progenitors arising from this diversification translocated from the Pacific into the Atlantic and established four geographically defined clonal subpopulations with remarkably low migration or mixing. Founders of two prevailing Atlantic subpopulations each acquired new stable and diagnostic inoviruses while other subpopulations that apparently declined did not. Broader surveys indicate inoviruses are common and active among the global population ofV. parahaemolyticusand though inovirus replacements, such as in ST36, appear to be infrequent, they are notable in pathogenic lineages that dispersed.ImportanceAn understanding of the processes that contribute to emergence of pathogens from environmental reservoirs is critical as changing climate precipitates pathogen evolution and population expansion. Phylogeographic analysis ofVibrio parahaemolyticushosts combined with analysis of theirInoviridaephage resolved ambiguities of diversification dynamics which preceded successful Atlantic invasion by the epidemiologically predominant ST36 lineage. It has been established experimentally that filamentous phage can limit host recombination, but here we show that phage loss is linked to rapid bacterial host diversification during epidemic spread in natural ecosystems alluding to a potential role for ubiquitous inoviruses in the adaptability of pathogens. This work paves the way for functional analyses to define the contribution of inoviruses in the evolutionary dynamics of environmentally transmitted pathogens.
Kinetochore dynein is sufficient to biorient chromosomes and remodel the outer kinetochore
Authors: Prevo Bram , Cheerambathur Dhanya K. , Earnshaw William C. , Desai Arshad 
Published: March 2023

ABSTRACTMultiple microtubule-directed activities concentrate on chromosomes during mitosis to ensure their accurate distribution to daughter cells. These activities include couplers and dynamics regulators localized at the kinetochore, the specialized microtubule interface built on centromeric chromatin, as well as motor proteins recruited to kinetochores and to mitotic chromatin. Here, we describe anin vivoreconstruction approach in which the effect of removing the major microtubule-directed activities on mitotic chromosomes is compared to the selective presence of individual activities. This approach revealed that the kinetochore dynein module, comprised of the minus end-directed motor cytoplasmic dynein and its kinetochore-specific adapters, is sufficient to biorient chromosomes and to remodel outer kinetochore composition following microtubule attachment; by contrast, the kinetochore dynein module is unable to support chromosome congression. The chromosome-autonomous action of kinetochore dynein, in the absence of the other major microtubule-directed factors on chromosomes, rotates and orients a substantial proportion of chromosomes such that their sister chromatids attach to opposite spindle poles. In tight coupling with orientation, the kinetochore dynein module drives removal of outermost kinetochore components, including the dynein motor itself and spindle checkpoint activators. The removal is independent of the other major microtubule-directed activities and kinetochore-localized protein phosphatase 1, suggesting that it is intrinsic to the kinetochore dynein module. These observations indicate that the kinetochore dynein module has the ability coordinate chromosome biorientation with attachment state-sensitive remodeling of the outer kinetochore that facilitates cell cycle progression.
Synthetic cell-cycle regulation identifies Mif2CENP-Cas a CDK phospho-target at the kinetochore
Authors: Klemm Cinzia , Ólafsson Guðjón , Thorpe Peter H. 
Published: March 2023

AbstractProtein phosphorylation regulates multiple cellular processes including cell-cycle progression, which is driven by highly conserved cyclin-dependent kinases (CDKs). CDKs are controlled by the oscillating levels of activating cyclins and the activity peaks during mitosis to promote chromosome segregation. However, with some exceptions, we do not understand how the multitude of CDK-phosphorylated residues within the proteome drive cell-cycle progression nor which CDK phosphorylation events are necessary. To identify yeast proteins whose phospho-regulation is most critical for cell-cycle progression, we created a synthetic CDK complex and systematically recruited this to proteins involved in chromosome segregation using the Synthetic Physical Interactions (SPI) method. We found that targeted recruitment of synthetic CDK to the centromeric protein Mif2CENP-Cleads to enrichment of Mif2CENP-Cat centromeres and arrested cells in late mitosis. We then identified putative CDK consensus sites on Mif2CENP-Cwhich aid Mif2CENP-Clocalisation at centromeres and showed that CDK- dependent Mif2CENP-Cphosphorylation is important for its stable kinetochore localisation.SummaryTo identify cellular sites of functional cell cycle phospho-regulation we generated a synthetic cyclin-dependent kinase which can be recruited to any given GFP-tagged protein. Using this system with a set of proteins involved in chromosome segregation, we identified Mif2CENP-Cas a kinetochore target of CDK and show that CDK stabilises Mif2’s kinetochore localisation.
Qualitative assessment of resilience in construction projects
Authors: Hilu Khalid A. , Hiyassat Mohammed A. 
Published: March 2023

Purpose
Due to the recent increase in unexpected events that negatively affect projects, the concept of resilience has grasped the attention of researchers, as risk management alone fails to address unpredicted events. This study aims to identify resilience dimensions and their designated factors and then construct a comprehensive definition for resilience in construction projects.

Design/methodology/approach
This paper adopted a qualitative research design through content analysis of semi-structured interviews with project engineers on behalf of their construction project; 26 interviews were analyzed via ATLAS.ti.

Findings
The results demonstrate that resilience in construction projects consists of four main dimensions: preparation, absorption, recovery and adaptation; each dimension comprises separate factors. The evidence from this study demonstrates that the resilience of the organization, project team and project manager supports the construction project when facing unpredicted or abnormal conditions.

Practical implications
The results of this paper will help construction project managers to enhance the resilience of their projects by providing different resilience factors.

Originality/value
The resilience concept in projects is novel and has limited knowledge available, especially in construction projects therefore, it requires additional comprehensive discussion and validation. The findings of this paper will serve as a cornerstone for the development of the resilience field and enrich the indigent literature on resilience in the construction industry.
Leveraging socio-technical processes to support researchers
Authors: Smith II Plato L. 
Published: March 2023

Purpose
The purpose of this project was to develop research support services that address local and external research data management (RDM) support drivers within the existing organizational culture at the University of Florida. The goal was to prompt organization change to support a campus-wide electronic lab notebook.

Design/methodology/approach
This project used a mixed-methods research approach to cultivate an organizational change program that support technological infrastructure to benefit researchers. The mixed-methods research involved participation action research integrated with a stakeholder approach.

Findings
The development of the grant proposal which was unfunded led to development of continued project goals. This project confirmed the development for support for an institution-wide electronic research notebook (ERN) solution requires adherence to the summary of five key actions for developing RDM services. Failure to complete all of the key actions engenders fragmentation culture.

Research limitations/implications
This project includes implications for institutions to develop grant proposals with integrated budgets for research support services of funded projects; and to use the summary of key actions for developing RDM services articulated by Jones et al. (2013) in “How to Develop RDM Services – a guide for HEIs.” Both are need to support findable, accessible, interoperable and reusable data for researchers.

Practical implications
This project has practical implications for higher education institutions interested in leveraging socio-technical processes to advance the role of libraries as collaborator, partner and stakeholder in developing institution-wide adoption, support and training for ERN as a research support service to RDM.

Social implications
This paper contributes to the body of developing literature on ERN as support services to RDM lead by academic research libraries.

Originality/value
This project contributed to the change in organization culture resulting in the successful collaboration between the Research Office and College of Medicine to support an institution-wide ERN technological infrastructure for one year as a pilot at a large academic research institution in the southeast USA.
The effect of co-patenting as a form of knowledge meta-integration on technological differentiation at Siemens
Authors: Ervits Irina 
Published: March 2023

PurposeThe paper proposes an answer to one of the most important questions in corporate innovation management: what mechanisms of technological diversification exist within multinational companies? It is ascertained that research and development (R&D) intra-firm co-invention or co-patenting is one of those mechanisms. Co-invention implies knowledge-sharing, which should lead to unique combinations of knowledge and expertise and hence technological diversification of patent applications.Design/methodology/approachThis paper offers a novel conceptual framework exploring the relationship between patents’ technological diversification and a detailed classification of different forms of international co-invention. Based on the case of Siemens’ Patent Cooperation Treaty (PCT) applications, the revealed technological advantage (RTA) index is utilized to measure the extent of the technological diversification of patent output.FindingsThe results show that patent applications generated by subsidiaries in advanced economies in cooperation with other subsidiaries feature unique technological areas that deviate from the company's overall technological specializations. These results provide a strong argument in favor of inter-subsidiary or horizontal co-patenting as a mechanism of new knowledge creation.Research limitations/implicationsOn the conceptual level, the results accentuate inter-subsidiary patenting being an important mechanism of knowledge meta-integration boosting technological diversification. The obvious limitation of this paper lies in exploring a single company case, which restricts the generalizability of our findings. Due to the dynamic nature of technological change, the author’s dataset also suffers from a lack of temporal external validity. Future research can expand the scope in both regards in applying our co-invention mode typology.Practical implicationsBased on the results, to diversify knowledge portfolio, companies should strengthen the co-patenting effort and reinforce horizontal (inter-subsidiary) R&D collaborations.Originality/valueTo the author’s knowledge, this is the first time when such a nuanced typology of co-invention modes is being utilized to understand the effect of different co-invention categories on knowledge diversification.
Adoption of service robots: exploring the emerging trends through the lens of bibliometric analysis
Authors: Madhan Kumar , Shagirbasha Shameem , Kumar Mishra Tanmaya , Iqbal Juman 
Published: March 2023

PurposeThe aim of this study is to examine the existing literature on service robots in order to identify prominent themes, assess the present state of service robotics research and highlight the contributions of seminal publications in the business, management and hospitality domain.Design/methodology/approachThis study analysed 332 Scopus papers from 1985 to 2022 using bibliometric techniques like citation and co-citation analysis.FindingsThe study findings highlighted that there has been a consistent rise in publications related to service robots. The paper identifies three significant themes in the service robot literature: adoption of service robots in the context of customer service, anthropomorphism and integration of artificial intelligence in robotic service. Furthermore, this study highlights prominent authors, journals, institutions and countries associated with research on service robots and discusses the future research opportunities in this domain.Originality/valueThis study contributes to the service robots’ literature in the hospitality context by compilation of various reference materials using a comprehensive bibliometric analysis. Previous studies do not point out crucial themes in this area, nor do they provide an overview of prominent journals, institutions, authors and trends in this field. Therefore, this study attempts to fill the lacunae.
Quiet quitting: relationship with other concepts and implications for tourism and hospitality
Authors: Hamouche Salima , Koritos Christos , Papastathopoulos Avraam 
Published: March 2023

Purpose
While quiet quitting is not an entirely new phenomenon, no published research has examined its relationship to existing concepts from a human resource management and organizational behavior perspective. Therefore, this study is a critical reflection that aims to demonstrate the relationship of quiet quitting with concepts researchers in tourism and hospitality have extensively used to study related phenomena.

Design/methodology/approach
Gray literature was mobilized to capture the momentum of this new phenomenon, whereas scholarly research was reviewed to identify existing concepts associated with quiet quitting and suggest directions for theory-building and empirical research.

Findings
In its contemporary form, quiet quitting mostly resonates with younger employees, due to the drastic changes in workplaces following the COVID-19 pandemic. While quiet quitting closely resembles collective industrial action such as “work to rule” and “acting one’s wage,” it also has a psychological dimension, and can be understood through concepts such as work withdrawal, employee cynicism, and silence. Multiple theories and concepts are proposed to facilitate the conceptualization and operationalization of quiet quitting (e.g. organizational citizenship behavior, social exchange, psychological contract, organizational justice, conflict theory, equity theory, two-factor theory, job demands-resources and conservation of resources theories).

Practical implications
This research provides practical suggestions to managers in tourism and hospitality to prevent the occurrence of quiet quitting in the first place, as well as effectively handling it once it occurs.

Originality/value
Studies addressing quiet quitting are rare. This paper attempts to synthesize diverse concepts and theories associated with quiet quitting to understand its meaning, potential causes and to suggest avenues for future research.