bluuebunny/crossref_metadata_2025_split huggingface dataset:
An analytical insight of discussions and sentiments of Indians on Omicron-driven third wave of COVID-19 using twitter data
Authors: Vatsa Deepika Vatsa Deepika , Yadav Ashima Yadav Ashima 
Published: February 2023

Abstract
Microblogging has become one of the most crucial tool for expressing and sharing the opinions and views of everyday life events. Digital channels are being used to monitor public health issues on the Internet. Twitter is a very popular source that provides tweets related to the sentiment of the public during the COVID-19 pandemic. Many researchers have used tweets to monitor the opinion of the people towards the coronavirus vaccine, mental health problems, treatment received by the doctors, impact of lockdown, etc. However, these works were mostly limited to the first and second waves of the pandemic. In this work, we aim to study the impact of the third wave of the pandemic, which started in December 2021 in India. We accomplished this by collecting tweet data set of two months, i.e., December 2021 and January 2022, discussing COVID-19 and having country code as "IN". We employed the Latent Dirichlet Allocation (LDA) technique for topic modeling and labeled each tweet message with the topic words that best describe it. We also utilized sentiment labels for each tweet and analyzed the distribution of different topics across different sentiment labels. This helped us to analyze the perspectives and sentiments of the people with respect to different topic discussions. Our analysis discovered that the two most discussed topics were ``precautionary measures" like get well soon, stay safe, wear mask, etc., and ``vaccine" where people have discussed about its effectiveness and vaccination drive in India. We found that people mostly had neutral sentiments for the former topic while for the latter, overall sentiment polarity was negative, reflecting peoples' mistrust in the COVID-19 vaccine.
Xeno-free generation of human induced pluripotent stem cells from donor-matched fibroblasts isolated from dermal and oral tissues
Authors: Ali Hassan R.W. , Suliman Salwa , Osman Tarig Al-Hadi , Carrasco Manuel , Bruland Ove , Costea Daniela-Elena , Ræder Helge , Mustafa Kamal 
Published: February 2023

Abstract
Background
Induced pluripotent stem cells (iPS) can be generated from various somatic cells and can subsequently be differentiated to multiple cell types of the body. This makes them highly promising for cellular therapy in regenerative medicine. However, to facilitate their clinical use and to ensure safety, iPS culturing protocols must be compliant with good manufacturing practice guidelines, and devoid of xenogenic products. Therefore, we aimed to compare the efficiency of using humanized culture conditions, specifically human platelet lysate to fetal bovine serum, for iPS generation from different sources, and to evaluate their stemness.
Methods
iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal, and gingival human fibroblasts, isolated from healthy donors (n=2) after informed consent, via episomal plasmid transfection. Pluripotency, genotype, and phenotype of iPS, generated by both protocols, was then assessed by various methods.
Results
More attempts were generally required to successfully reprogram xeno-free fibroblasts to iPS, as compared to xenogenic cultured fibroblasts. Furthermore, oral fibroblasts generally required more attempts for successful iPS generation as opposed to dermal fibroblasts. Morphologically, all iPS generated from fibroblasts formed tight colonies surrounded by a reflective "whitish" outer rim, typical for iPS. They also expressed pluripotency markers at both gene (SOX2, OCT4, NANOG) and protein level (SOX2, OCT4). Upon stimulation, all iPS showed ability to differentiate into the three primary germ layers via expression of lineage specific markers for mesoderm (MESP1, OSR1, HOPX), endoderm (GATA4), and ectoderm (PAX6, RAX). Genome analysis revealed several amplifications and deletions within the chromosomes of each iPS cell line.
Conclusions
The xeno-free protocol had a lower reprogramming efficiency compared to the standard xenogenic protocol. The oral fibroblasts generally proved to be more difficult to reprogram than dermal fibroblasts. Xeno-free dermal, buccal, and gingival fibroblasts can successfully generate iPS with a comparable geno/phenotype to their xenogenic counterparts.
Clinical and microbiological outcomes of subgingival instrumentation supplemented with high-dose omega-3 polyunsaturated fatty acids in periodontal treatment – a randomized clinical trial
Authors: Stańdo-Retecka Mirella , Piatek Paweł , Namiecinska Magdalena , Bonikowski Radosław , Lewkowicz Przemyslaw , Lewkowicz Natalia 
Published: February 2023

Abstract
Purpose

This study aimed to evaluate the impact of dietary supplementation with omega-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) combined with scaling and root planning (SRP) in untreated periodontitis stage III and IV.

Methods

Forty patients were randomly assigned to test group receiving SRP plus omega-3 PUFAs (n = 20) and control group receiving SRP alone (n = 20). Clinical changes of pocket probing depths (PD), clinical attachment level (CAL), bleeding on probing (BOP) and proportions of closed pockets (PPD ≤ 4 mm without BOP) were evaluated at baseline and after 3 and 6 months. Subgingival biofilm Phorphyromonas gingivalis, Tanarella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans counts were analysed at baseline and at 6 months. Serum was subjected to lipid gas chromatography/mass spectrometry analysis at baseline and at 6 months.

Results

Significant improvement of all clinical parameters over 3 and 6 months was observed in both groups. For the primary outcome “change of mean PD,” no significant differences were detected between the groups. Patients treated with omega-3 PUFAs demonstrated significantly lower rates of BOP, higher gain of CAL and higher number of closed pockets at 3 months in comparison to the control group. After 6 months, no clinical differences between the groups were found, with exception of lower BOP rates. Moreover, in the test group, the number of key periodontal bacteria was significantly lower than in the control group at 6 months. Increased proportions of serum n-3 PUFAs and decreased proportions of n-6 PUFAs in the patients form the test group were detected at 6 months.

Conclusion

High-dose omega-3 PUFA intake during non-surgical treatment of periodontitis results in short-term clinical and microbiological benefits.
The study protocol was approved by the ethical committee of Medical University of Lodz (reference number RNN/251/17/KE) and registered at clinicaltrials.gov (NCT04477395) on 22/07/2020.
Resculpting Carbon dots via electrochemical etching
Authors: Yang Qingsong , Gavalas Spyridon , Ejsmont Aleksander , Krysmann Marta J. , Guo Jiangtao , Li Li , Guo Xuhong , Kelarakis Antonios 
Published: February 2023

Abstract
Substantial efforts are directed into exploring the structure-properties relationships of photoluminescent Carbon dots (C-dots). This study unravels a resculpting mechanism in C-dots that is triggered by electrochemical etching and proceeds via extensive surface oxidation and carbon-carbon breakage. The process results in the gradual shrinkage of the nanoparticles and can enhance the quantum yield by more than half order of magnitude compared to the untreated analogues.
Typical Metabolic Pattern of 18F-FDG PET in Anti-NMDAR Encephalitis in the Acute and Subacute Phases and its correlation with T2 FLAIR-MRI features
Authors: Yuan Leilei , Mao Guangjuan , Zhang Yudi , Xu Yang , Chen Qian , Shan Baoci , Cui Tao , Ai Lin 
Published: February 2023

Abstract
Background/aims: Early diagnosis of Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis with non-invasive imaging modalities benefiting is crucial to guarantee prompt treatments decision-making and good prognosis for patients. The present study aimed to explore the correlation of MRI features with brain metabolism characteristics of 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET) and to describe the metabolic patterns in Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis at acute or subacute phase. Twenty-four patients with anti-NMDAR encephalitis confirmed by serum and/or CSF tests at acute or subacute phase, 9 females and 15 males, with an age range of 6-80 years, were enrolled in this retrospective study as encephalitis group. 18F-FDG PET and MRI findings of all patients were investigated and interpreted with visual analysis. Chi-square test was performed to compare the diagnostic sensitivity between MRI and PET. Independent sample t-test was used to compare the standardized uptake value ratio (SUVR) of each ROI between the encephalitis group and control group, which consisted of 24 age- and gender-matched healthy volunteers.
Results: The diagnostic sensitivity of FDG PET (23/24, 95.83%) was higher than that of MRI (18/24, 75.00%) in acute or subacute anti-NMDAR encephalitis patients with statistically significant difference (P＜0.05). Three categories of abnormalities shown on T2 FLAIR, including shallow of sulci and swelling of brain tissue, increased signal in the sulci, increased signal on brain gray matter or adjacent white matter presented hypermetabolism on PET, excepting increased signal in brain linear structure with hypometabolism of the basal ganglia on PET.
Conclusion: Anteroposterior glucose metabolism gradient (frontal-temporal/parietal-occipital) is proved to be a typical pattern of anti-NMDAR encephalitis at the acute and subacute phases in both visual and statistical testing. Interestingly, the pattern is also commonly found in the anterior and posterior portions of the parietal lobe and cingular cortex, which may be a potential indicator for the diagnosis of this disorder. In addition, MRI is an important and reliable neuroimaging modality to assist in the correct evaluation of activity changes on 18F-FDG PET.
A spatiotemporal dissolved oxygen prediction model based on graph attention networks suitable for missing data
Authors: Fang Yamin , Liu Hui 
Published: February 2023

Abstract
The evaluation and forecasting of water quality are crucial for the prevention and management of water pollution. The majority of the current water quality prediction techniques only take into account a single site. Moreover, there are a huge number of missing values in the monitored water quality data, which prevents the model from functioning correctly. To address these problems, a spatiotemporal prediction framework based on graph attention network (GAN) appropriate for missing data is developed. First, a module based on Neural Controlled Differential Equations (NCDE) is employed to deal with missing data. Then, an improved GAN is utilized to establish the spatiotemporal link of the data on water quality. A new iterative optimization method based on a k-nearest neighbor graph is proposed to enhance the performance of the graph model. To enhance the model's ability to handle multiple features, Shapley additive explanations (SHAP) is applied to evaluate the importance of features, and then multiple features is screened, and they are fused through GRU. Moreover, a fused graph attention mechanism is proposed to improve the robustness of GAN against noise. According to the experimental results, the proposed framework outperforms other models, and the model maintains stable performance for missing data. The spatiotemporal prediction method established in this paper can be a potential strategy to expand the water quality prediction model library.
A Box-Behnken design-based chemometric approach to optimize the degradation of hydroxychloroquine in water media using the Fe(0)/S2O2-8/UV system
Authors: BENDJEFFAL Hacene , Mamine Hadjer , Boukachabia Mourad , Aloui Amel , Metidji Toufek , Djebli Abdelkrim , Bouhedja Yacine 
Published: February 2023

Abstract
The huge utilization of hydroxychloroquine in autoimmune infections led to an abnormal increment in its concentration in wastewater, which can pose a real risk to the environment, necessitating the development of a pretreatment technique. In order to do this, we are interested in researching how hydroxychloroquine degrades in contaminated water. The main goal of this investigation is to optimize the operating conditions for the hydroxychloroquine degradation in water using Fe(0)/S2O2-8/UV system. To get adequate removal of HCQ, a chemometric method based on the Box–Behnken design was applied to optimize the influence of the empirical parameters selected including Fe(0) dose, S2O2-8 concentration, pH, and initial HCQ concentration. The quadratic regression model representing the HCQ removal rate (η%) was evolved and validated by ANOVA. The optimal conditions as a result of the above-mentioned trade-off between the four input variables, with η(%) as the dependent output variable, were captured using RSM methodology and the composite desirability function approach. For HCQ full decomposition, the optimal values of the operating factors are as follows: S2O2-8 dose: 194.309 mgL-1; Fe(0) Quantity: 198.83mgL-1 ; pH=2.017 and HCQ initial dose of 296.406mgL-1. Under these conditions, the HCQ removal rate, achieved after 60 minutes of reaction, attained 98.95%.
Strategy with machine learning models for precise assembly using programming by demonstration
Authors: Bai Ye , Hsieh Sheng-Jen 
Published: February 2023

Abstract
Programming by Demonstration(PBD) applies in the industry as a method of human-robot collaboration for assembly, such as placing, inserting, and screwing; however, PBD has not received much attention in commercial electronic product assembly. There is little work on integrating a strategy to optimize trajectory planning, which is an initial step of PBD. Most recent related works improving PBD performance focus on taking care of numerous demonstration samples from motion-sensing devices under time consumption, distance, and other related criteria. However, it is necessary to integrate a strategy to optimize the performance of precise assembly tasks. We proposed a framework with two custom algorithms to pre-process and classify contactless demonstration performance in this research work. It verified that these algorithms could more effectively generate optimal motion paths in criteria of distance, smoothness, and trajectory variance than canonical methods. Machine learning methods, including the Convolutional neural network(CNN), Artificial Neural Network(ANN), and Support Vector Machine(SVM), are feasible to predict the further performance that the best motion path with accuracy ranging from 80% to 85% accuracy. Also, CNN performs better than ANN and SVM. Among CNN methods, the DarkNet yields the highest accuracy rate of 85%. Future work includes the hybrid CNN/ANN algorithm, which may yield higher accuracy in prediction. Also, the proposed algorithms may apply to robots with dual assembly arms which mimic human arms.
Highly Efficient Biosynthesis of Salidroside by a UDP-glucosyltransferase-catalyzed Cascade Reaction
Authors: Li Guosi , Xu Qilin , Hu Nan , Liu Xinyang , Jiang Yiqi , Xue Hailong , Zang Yongjun , Zhu Fucheng 
Published: February 2023

Abstract
Objective Salidroside is an important plant-derived aromatic compound with diverse biological properties. The main objective of this study was to synthesize salidroside from tyrosol using UDP-glucosyltransferase (UGT) with in situ regeneration of UDP-glucose (UDPG).
Results The UDP-glucosyltransferase 85A1 (UGT85A1) from Arabidopsis thaliana,which showed high activity and regioselectivity towards tyrosol, was selected for the production of salidroside. Then, an in vitro cascade reaction for in situ regeneration of UDPG was constructed by coupling UGT85A1 to sucrose synthase from Glycine max (GmSuSy). The optimal UGT85A1-GmSuSy activity ratio of 1:2 was determined to balance the efficiency of salidroside production and UDP-glucose regeneration. Different cascade reaction conditions for salidroside production were also determined. Under the optimized condition, salidroside was produced at a titer of 6.0 g/L with a corresponding molar conversion of 99.6% and a specific productivity of 199.1 mg/L/h in a continuous feeding reactor.
Conclusion This is the highest salidroside titer ever reported so far using biocatalytic approach.
Attenuated Dengue Virus PV001-DV Induces Oncolytic Tumor Cell Death and Potent Immune Responses
Authors: Goldufsky Josef W. , Daniels Preston , Williams Michael D. , Gupta Kajal , Lyday Bruce , Chen Tony , Singh Geeta , Kaufman Howard L. , Zloza Andrew , Marzo Amanda Lee 
Published: February 2023

Abstract
Background
Viral therapies developed for cancer treatment have classically prioritized direct oncolytic effects over their immune activating properties. However, recent clinical insights have challenged this longstanding prioritization and have shifted the focus to more immune-based mechanisms. Through the potential utilization of novel, inherently immune-stimulating, oncotropic viruses there is a therapeutic opportunity to improve anti-tumor outcomes through virus-mediated immune activation. PV001-DV is an attenuated strain of Dengue virus (DEN-1 #45AZ5) with a favorable clinical safety profile that also maintains the potent immune stimulatory properties characterstic of Dengue virus infection.
Methods
In this study, we utilized tumor killing and immune multiplex assays to examine the anti-tumor effects of PV001-DV as a potential novel cancer immunotherapy.
Results
In vitro assays demonstrated that PV001-DV possesses the ability to directly kill human melanoma cells lines as well as patient melanoma tissue ex vivo. Importantly, further work demonstrated that, when patient peripheral blood mononuclear cells (PBMCs) were exposed to PV001-DV, a substantial induction in the production of apoptotic factors and immunostimulatory cytokines was detected. When tumor cells were cultured with the resulting soluble mediators from these PBMCs, rapid cell death of melanoma and breast cancer cell lines was observed. These soluble mediators also increased dengue virus binding ligands and immune checkpoint receptor, PD-L1 expression.
Conclusions
The direct tumor-killing and immune-mediated tumor cytotoxicity facilitated by PV001-DV contributes support of its upcoming clinical evaluation in patients with advanced melanoma who have failed prior therapy.
Empiric transarterial embolization in angiographically negative lower gastrointestinal bleeding using vessel tracking and 3D navigation tools: report of 2 patients
Authors: Hermie Laurens , Defreyne Luc 
Published: February 2023

Abstract
Background: Recently, an empiric Cone-beam Computed Tomography (CBCT)-guided transarterial embolization (TAE) technique has been investigated for lower gastrointestinal bleeding (LGIB). Although this empirical strategy reduced the rate of rebleeding in hemodynamically unstable patients compared to a ‘wait and see’ strategy, the specified technique is challenging and time-consuming.
Case presentation: We present two methods to perform a prompt empiric TAE in LGIB when catheter angiography is negative. Based on the pre-procedural Computed Tomography Angiography bleeding site and using vessel detection and navigation software tools that are integrated in contemporary angiosuites, the culprit bleeding artery could be targeted with only one selective intraprocedural CBCT acquisition.
Conclusion: The proposed techniques is promising to reduce procedure time and facilitate the implementation of empiric CBCT-guided TAE in clinical practice when angiography is negative.
Variability of Land Tenure, Land Rights and Land Acquisition Methods Among Male and Female Farmers in Irrigation Sector of Gombe and Bornu States, Nigeria
Authors: Ayoola J. B. , Sani R. M. , Ayoola G. B. , Dzever D. , Zaman E. Y. , Sani M. , Adoka T. , Moses A. U. , Yunusa M.Y. , Abu G. A. , Abu O. 
Published: February 2023

Abstract
This study determined the variability of socio-economic characteristics, land rights, land tenure and method of land acquisition among land users in the Dadin kowa irrigation scheme area of Gombe and Borno States, Nigeria. The study adopted a multi-stage sampling technique; 2 LGAs, 6 villages and 300 households were systematically selected in stages. Households specific information was collected using structured questionnaires. A combination of analytical tools employed in the analyses includes descriptive statistics (means, frequencies and percentages), and inferential statistics (t/z tests). The results showed that 29.0% of the respondents were aged 31–40 years; while those within the ages of 20–30, 41–50, 51–60 and above 60 years age brackets constituted 17.0%, 16.3%, 16.0% and 19.3% respectively. Similarly, the sex segregation of households shows that majority of the respondents (95.3%) were male while 4.7% of the respondents were of the female category. Similarly, 24.7% of the respondents had household size of 7–10 persons while 21.0% and 20.0% had a household size of 3–6 and 11–14 persons respectively. Also, 61.3% of the respondents belong to one form of cooperative body or the other. The results indicated that, the rights include the rights to sell, rent, graze, bequeath, and use as religious center. The land tenure systems include family inheritance (92.3%), individual ownership by purchase (79%), renting (78%); while individual ownership through gift, leasing, and government allocation ranked 4th ,5th and 6th respectively. Land acquisition by inheritance was most common among males than females (69.3 and 27.7% respectively); the chi-square value indicated significant variability at 1% probability level. Similarly, land acquisition through buying, renting and through government allocation was more common among the male users (59.5, 58.8, and 22.3%) correspondingly. Results also indicated an average farm size of 0.9 ha and 0.3ha for male and female respectively, and a significant difference with T-value of -4.231 at 1% probability. The study concluded that women are more involved in agriculture in the considered gender categories. The most common land ownership structure among both females and males is inheritance. Land holding in number of plots and farm size are higher among males than females in the study area. Recommendations include the need for gender mainstreaming in transforming the irrigation systems, enlightenment of land users to embrace membership of farmers associations and more women friendly agro practices should be the focus of extension services.