bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer
Authors: Guo Yuanli , He Xinxin , Liu Junfeng , Tan Yanming , Zhang Chao , Chen Shan , Zhang Sheng 
Published: January 2025

IntroductionPrimary cilia play an important role in the development of cancer by regulating signaling pathways. Several studies have demonstrated that women with BRCA mutations have, on average, 50% fewer ciliated cells compared with general women. However, the role of tubal cilia loss in the development of epithelial ovarian cancer (EOC) remains unclear. Few specific studies have been found in linking HYDIN, a ciliary defect associated gene that encodes HYDIN axonemal central pair apparatus protein, which is involved in the transduction of Hedgehog (Hh) signal and is considered a cancer associated antigen, to ovarian cancer. Therefore, our study aimed to investigate the correlation between HYDIN gene mutations and tubal cilia loss in EOC.MethodsA whole exome sequencing (WES), immunohistochemistry (IHC), western blot, and reverse transcription quantitative (RT q) PCR were performed in 80 patients with EOC and 50 cases of non ovarian cancer to detect the mutations and expression of tubal ciliary marker, ciliary morphology, and abnormal rate.ResultsWe found that the incidence of tubal cilia loss was higher in EOC group with decreased expression of HYDIN compared with the control group (P&lt;0.05).DiscussionThis study suggests that tubal ciliary loss is evident in epithelial fallopian tube carcinoma, and ciliary cells may be involved in the occurrence and development of EOC, and cilia-related gene HYDIN is expected to be a tumor marker for epithelial ovarian cancer.
Adverse drug events (ADEs) risk signal mining related to eculizumab based on the FARES database
Authors: Wang Xi-Feng , Bao Lu-Ri , Hu Ta-La , Xu Rui-Feng , Gao Wu-Niri , Wang Jing-Yuan , Zhao Jian-Rong , Fu Zhen-Li , Wang Shu-Fang , Meng Yan 
Published: January 2025

IntroductionEculizumab is a C5 complement inhibitor approved by the FDA for the targeted treatment of four rare diseases, paroxysmal nocturnal hemoglobinuria (PNH), atypical hemolytic uremic syndrome (aHUS), generalized myasthenia gravis (gMG), and aquaporin-4 immunoglobulin G-positive optic neuromyelitis optica spectrum disorders (AQP4-IgG+NMOSD). The current study was conducted to assess real-world adverse events (AEs) associated with eculizumab through data mining of the FDA Adverse Event Reporting System (FAERS).MethodsDisproportionality analyses, including Reporting Ratio Ratio (ROR), Proportional Reporting Ratio (PRR), Bayesian Confidence Propagation Neural Network (BCPNN), and Multi-Item Gamma Poisson Shrinker (MGPS) algorithms were used to quantify the signals of eculizumab-associated AEs.ResultsA total of 46,316 eculizumab-related ADEs reports were identified by analyzing 19,418,776 reports in the U.S. Food and Drug Administration Adverse Event Reporting System (FAERS) database. A total of 461 PTs were identified as satisfying by all four algorithms. These PTs reported adverse reactions consistent with the specifications, such as fatigue, nasopharyngitis, meningococcal infection, fever, and anemia. Some PTs, such as aplastic anemia, gene mutation, mastication disorder, kidney fibrosis, BK virus infection, abnormal neutrophil count, C3 glomerulopathy, neuroblastoma, and glomerulonephritis membranoproliferative, were also detected outside the instructions. The median time to onset of eculizumab adverse events was 159 days (interquartile range [IQR] 11∼738 days). In addition, at the PT level, 51 PTs were determined to have an imbalance in the occurrence of ADEs between the sexes.ConclusionThese findings provide valuable insights into the occurrence of ADEs following the use of eculizumab and could support clinical monitoring and risk identification efforts.
Protective effects of berbamine against arginase-1 deficiency-induced injury in human brain microvascular endothelial cells
Authors: Wei Xiaolan , Li Weiwei , Chen Zixuan , Chen Jintu , Chen Yun , Cai Jiangping , Lin Huasong 
Published: January 2025

Endothelial cell dysfunction plays a crucial role in the early development of cerebral small vessel disease (CSVD). Arginase-1 (ARG1) is expressed in endothelial cells, and its deficiency may exacerbate cerebrovascular damage by increasing reactive oxygen species (ROS) production, thereby inducing endothelial cell apoptosis. Berbamine (BBM) has shown potential in neuroprotection and cardiovascular disease prevention. This study aimed to investigate the impact of ARG1 deficiency on human brain microvascular endothelial cells and the protective effects of BBM against ARG1 deficiency-induced damage. Human brain microvascular endothelial cells (HCMEC/D3) were cultured in vitro, and ARG1 knockdown or overexpression was achieved using plasmid transfection techniques. We examined the effects of ARG1 expression levels on HCMEC/D3 cell viability, migration, apoptosis, adhesion, and angiogenesis through cellular experiments. Additionally, we explored how ARG1 expression levels influenced arginine (Arg), nitric oxide (NO), and ROS levels in HCMEC/D3 cells. The results demonstrated that ARG1 deficiency inhibited HCMEC/D3 cell viability, migration, adhesion, and angiogenesis, while promoting apoptosis and elevating Arg, NO, and ROS levels in HCMEC/D3 cells. Next, the effect of different BBM concentrations on HCMEC/D3 cell viability was assessed using the CCK-8 assay, revealing that BBM at a concentration of 5 µM had no significant impact on cell viability. Subsequently, after successfully knocking down ARG1 in HCMEC/D3 cells, the cells were treated with BBM. The results showed that BBM effectively mitigated the negative effects of ARG1 deficiency on HCMEC/D3 cell viability, migration, apoptosis, adhesion, and angiogenesis, while also reducing Arg, NO, inducible nitric oxide synthase (iNOS), and ROS levels in HCMEC/D3 cells. In conclusion, this study suggests that ARG1 deficiency may damage HCMEC/D3 cells by increasing Arg levels, leading to elevated NO and ROS levels. BBM may provide protection to ARG1-deficient HCMEC/D3 cells by reducing Arg, NO, iNOS, and ROS levels. These findings deepen our understanding of the pathogenesis of CSVD and provide a theoretical basis for the clinical application of BBM.
UGT2B15 single nucleotide polymorphism reduces dabigatran acylglucuronide formation in humans
Authors: Park Jin-Woo , Kim Jong-Min , Bang Young Yoon , Kim Kyoung-Ah , Yu Sungwook , Park Ji-Young 
Published: January 2025

BackgroundDabigatran etexilate (DABE), a prodrug of dabigatran (DAB), is a direct thrombin inhibitor used to prevent ischemic stroke and thromboembolism during atrial fibrillation. The effect of genetic polymorphisms on its metabolism, particularly UGT2B15, has not been extensively explored in humans. This study aimed to investigate the effects of UGT2B15, ABCB1, and CES1 polymorphisms on the pharmacokinetics of DAB and its acylglucuronide metabolites in healthy subjects.MethodsA total of 124 healthy males were genotyped for UGT2B15, ABCB1, and CES1 polymorphisms. After a single 150 mg dose of DABE, plasma concentrations of total and free DAB, as well as dabigatran acylglucuronide (DABG) were measured using LC-MS/MS. Pharmacokinetic parameters were analyzed using non-compartmental methods, and statistical comparisons were conducted between the genotype groups.ResultsUGT2B15 c.253G&gt;T significantly affected free DAB pharmacokinetics, with a lower Tmax and oral clearance in TT genotype (n = 28, p &lt; 0.05). For DABG, Cmax was significantly higher in GG genotypes (n = 32, 42.3 ± 16.3 ng/mL) compared to that in GT (n = 64, 32.4 ± 20.5 ng/mL) and TT (29.7 ± 17.1 ng/mL) genotypes. Similarly, the AUCall of DABG was highest in GG genotypes (327 ± 148.3 ng h·mL-1), followed by GT (238.7 ± 166.5 ng h·mL-1) and TT (223.3 ± 165.4 ng h·mL-1) genotypes (p &lt; 0.05). The metabolite-to-parent ratios (m/p ratios) for Cmax and AUCall were significantly higher in GG and GT genotypes than that in TT genotype. ABCB1 and CES1 polymorphisms had no significant impact on the pharmacokinetics of DAB or DABG.ConclusionUGT2B15 polymorphisms were associated with difference in DAB glucuronidation and pharmacokinetics in healthy male participants.
Polydatin enhances oxaliplatin-induced cell death by activating NOX5-ROS-mediated DNA damage and ER stress in colon cancer cells
Authors: Zhao Qi , Zhang Yan , Liu Jieyu , Chen Peipei , Onga Annabeth , Cho Namki , Cui Ri , Zheng Chenguo 
Published: January 2025

BackgroundPolydatin (3,4′,5-trihydroxy-3-β-d-glucopyranoside, PD) is known for its antioxidant and anti-inflammatory properties. Oxaliplatin (OXA)-based chemotherapy is the first-line treatment for metastatic and recurrent colorectal cancer (CRC). However, the lack of selectivity for normal cells often results in side effects. Consequently, the search for anti-cancer components with high efficacy and low cytotoxicity has become a significant focus in recent years.MethodsThe anti-tumor effects of PD, OXA or their combination were assessed by cell viability, colony formation, and wound-healing assays. Reactive oxygen species (ROS) generation was measured by flow cytometry and DNA damage was assessed by immunofluorescence assay. The relative gene and protein expressions were analyzed by quantitative real time-PCR (qRT-PCR) and Western blot assays. Molecular docking analysis predicted the interaction between PD and potential targets.ResultsWe found that PD exerted anti-CRC activity by promoting Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase 5 (NOX5)-mediated ROS production, activating the endoplasmic reticulum (ER) stress, and inducing DNA damage. Knocking down NOX5 attenuated the inhibition of proliferation and colony forming ability induced by PD in colon cancer cells and reversed the expression of C/EBP-homologous protein (CHOP) and activating transcription factor 4 (ATF4) proteins. In addition, combination of PD and OXA synergistically exerted anti-CRC activities by promoting DNA damage and activating ER stress signaling pathway.ConclusionThe combination of PD and OXA could be an effective treatment strategy for certain patients with CRC.
The effects of eccentric phase tempo in squats on hypertrophy, strength, and contractile properties of the quadriceps femoris muscle
Authors: Kojic Filip , Mandic Danimir , Duric Sasa 
Published: January 2025

IntroductionThe aim of the present study was to investigate the effects of eccentric phase tempo in squats on hypertrophy, strength, and contractile properties of the quadriceps femoris (QF) muscle.MethodsEighteen participants (10 males and 8 females, age 24.0 ± 1.7 years) with no resistance training (RT) experience in the last 8 months were randomized into two groups, each following a 7 week squat resistance training (RT) protocol with either a fast eccentric (FE, 1 s eccentric/0 s isometric/1 s concentric/0 s isometric) or slow eccentric (SE, 4 s eccentric/0 s isometric/1 s concentric/0 s isometric) tempo. The training intensity (60%–70% RM), the number of sets (3–4), and the rest intervals (120 s) were consistent in both groups. The study measured changes in quadriceps cross-sectional area (CSA), one-repetition maximum (1RM) strength, and muscle contractile properties such as contraction time (Tc) and radial displacement (Dm), using tensiomyography (TMG). An ANCOVA model with baseline values as covariates was used to examine between-group differences.ResultsResults showed significant strength gains in both groups, with the SE group achieving greater 1RM increases (effect size [ES] = 1.60 vs 0.99, p &lt; 0.05). CSA increased for all QF muscles; however, the SE group exhibited significantly higher hypertrophy in the vastus lateralis (ES = 1.74 vs. 1.37, p &lt; 0.05). TMG analysis revealed decreased Dm in the rectus femoris for both groups (p &lt; 0.05), while Tc significantly (ES = 1.33, p &lt; 0.01) increased in the SE group.DiscussionThese findings suggest that slower eccentric tempo in RT may optimize vastus lateralis hypertrophy and enhance strength while promoting muscle fiber-type specificity, contributing to the understanding of eccentric training’s role in muscle adaptation.
A study on the relationship between physical exercise and feelings of inferiority among college students: the chain mediating effect of social support and emotional regulation ability
Authors: Peng Bo , Chen Weisong , Wang Hongshen , Yu Ting , Kong Mingmin 
Published: January 2025

ObjectiveThis study aims to explore the impact of physical exercise on feelings of inferiority among college students, focusing on the mediating roles of social support and emotional regulation ability. The research investigates both direct and indirect pathways to understand how physical exercise enhances psychological resilience and mitigates negative self-perceptions.MethodsA sample of 2,036 college students from 15 provinces in China was surveyed using validated scales for physical exercise, feelings of inferiority, social support, and emotional regulation ability. Structural equation modeling (SEM) was employed to test hypothesized pathways, including direct effects and chain mediation effects. Gender invariance was assessed through multi-group analysis to ensure consistency across male and female participants.ResultsPhysical exercise significantly reduces feelings of inferiority, both directly and indirectly. Social support and emotional regulation ability independently mediate this relationship, with social support further enhancing emotional regulation in a sequential chain mediation effect. Gender invariance testing confirmed that these relationships are consistent for male and female students. Key findings indicate that physical exercise fosters social connections and strengthens emotional regulation, collectively promoting psychological resilience and reducing feelings of inferiority.ConclusionThe findings emphasize the critical role of physical exercise in enhancing mental health among college students through social and emotional pathways. Holistic intervention programs incorporating physical activities with social interaction and emotional skill-building can effectively alleviate feelings of inferiority. These insights provide actionable recommendations for educational institutions to promote mental health and foster inclusive, supportive campus environments.
Impact of crop commercialization on multidimensional poverty in rural Ethiopia: propensity score approach
Authors: Eyasu Anteneh Mulugeta , Zewotir Temesgen , Dessie Zelalem G. 
Published: January 2025

IntroductionReducing poverty through crop commercialization is one of the antipoverty efforts that helps promote health. This study explored the prevalence and the causal relationship between crop commercialization and rural Ethiopian households’ multidimensional poverty using multilevel data.MethodsThe study uses data from the most recent nationally representative Ethiopian socioeconomic survey 2018/19 to calculate the rural multidimensional poverty index using the Alkire and Foster technique. The data show 2,714 rural households nested in 59 administrative zones of Ethiopia. Based on several parameters (nutrition and health, education, living standards, rural livelihoods and resources, and risk), the investigation looks into the multidimensional poverty levels of Ethiopian rural households and how they differ across Ethiopian administrative zones.ResultsThe results indicate that 47.8% of the rural households of Ethiopians were multidimensionally poor in several dimensions; nutrition and health, education, living standards, rural livelihoods and resources, and risk. The living standard dimension is most deprivation-prone for the rural, multidimensional poor households. In addition, multidimensional poverty is more prevalent in Somali and Afar region rural areas. The best linear unbiased prediction estimates of multidimensional poverty vary substantially across Ethiopia’s administrative zones. Specifically, the top poorest performing administrative zones concerning the likelihood of being multidimensional poor among rural households were Shebelle, Zone 2, Zone 3, Zone 4, and Konso special woreda.ConclusionThe results of the generalized linear mixed-effects model show that crop-commercialized households have reduced the odds of being multidimensionally poorer than those who did not. This study recommends policymakers focus on rural mumyltidimensional poverty reduction strategies.
Relationship between emotional labor strategies and the quality of work life of operating room nurses
Authors: Li Li , Cao Yingjuan , Sun Yinuo , Li Yue , Zhai Yonghua , Yan Yan 
Published: January 2025

IntroductionEmotional labor involves regulating one’s emotional state at work to align with organizational expectations. For operating room (OR) nurses, emotional labor is an inherent part of their roles, with different strategies potentially impacting their work-related quality of life (WRQoL) in distinct ways. This study aimed to examine the relationship between emotional labor strategies and WRQoL among OR nurses.MethodsA cross-sectional study was conducted using convenience sampling, recruiting 395 OR nurses from 11 secondary and tertiary hospitals in Shandong Province. Data were collected using the General Information Questionnaire, Emotional Labor Scale, and Work-Related Quality of Life scale. Statistical analyses, including descriptive statistics, Pearson correlation analysis, and multiple stratified regression, were applied to explore the relationships between variables.ResultsA total of 372 valid responses were obtained, with an effective response rate of 94.18%. The mean WRQoL score was 114.17 (SD = 16.54). Among emotional labor strategies, expressing naturally felt emotions was the most frequently used. Surface acting showed a significant negative correlation with WRQoL (r = −0.437, p &lt; 0.05), while deep acting and expressing naturally felt emotions were positively correlated with WRQoL (r = 0.291 and r = 0.457, respectively, p &lt; 0.05). Multiple stratified regression analysis confirmed that emotional labor strategies significantly influenced WRQoL.ConclusionEmotional labor strategies play a crucial role in shaping the WRQoL of OR nurses. Nursing managers should prioritize assessing nurses’ emotional labor status and provide targeted guidance to promote positive strategies, such as deep acting and expressing naturally felt emotions. Such measures can enhance nurses’ physical and mental well-being, ultimately improving their quality of work life.
Validation of a tri-axial accelerometer for measuring physical activity in patients with subacute stroke
Authors: Kimura Yosuke , Suzuki Yoshiki , Kubo Hiroki , Yoshida Keishi , Ota Tomohiro , Shimizu Natsuki , Kanai Masashi 
Published: January 2025

PurposeThis study aimed to validate the accuracy of the Active Style Pro HJA-750C (ASP) in measuring metabolic equivalents (METs) during walking and reaching tasks in individuals with subacute stroke using a respiratory gas analyzer as a reference.MethodsTwenty-three hospitalized patients with subacute stroke participated in this study. They performed sitting and standing reaching tasks, as well as walking while wearing a VO2 Master respiratory gas analyzer and ASP devices on both the paretic and non-paretic sides. The METs values recorded by the ASP were compared with those obtained using a VO2 Master respiratory gas analyzer. Pearson's correlation coefficients were calculated for each task, and Bland–Altman plots were used to assess the agreement between the two methods.ResultsThe ASP demonstrated good concurrent validity, with correlation coefficients of 0.71 and 0.74 for the sitting reaching task, 0.75 and 0.79 for the standing reaching task, and 0.83 and 0.85 for walking when the ASP was placed on the paretic and non-paretic sides, respectively. Bland–Altman analysis indicated no significant fixed or proportional errors. The ASP accurately measures METs whether worn on the affected or unaffected side of the waist.ConclusionThe ASP provides valid measurements of physical activity during walking and reaching tasks in patients with subacute stroke. These findings suggest that ASP is a valuable tool for monitoring physical activity in clinical rehabilitation settings.
Sustainability-driven fertilizer recommender system for coffee crops using case-based reasoning approach
Authors: León Chilito Edinson David , Casanova Olaya Juan Fernando , Corrales Juan Carlos , Figueroa Cristhian 
Published: January 2025

IntroductionIn recent years, the increased demand for food has prompted farmers to increase production to support economic expansion. However, the excessive use of mineral fertilizers poses a significant threat to the sustainability of food systems. In Colombia, coffee cultivation plays a fundamental role in the economy, thus creating a recognized demand to elevate its production while minimizing its environmental impact sustainably.MethodologyThe study follows the CRISP-DM methodology (Cross-Industry Standard Process for Data Mining) developing of a fertilizer recommender system (FRS) for coffee crops. This process includes business understanding, where the key factors influencing coffee production were identified; data understanding and preparation, where agroclimatic data and expert knowledge were collected and processed; modeling, which involved building a case-based reasoning (CBR) system to recommend fertilizer doses and frequencies, and evaluation, where expert feedback was gathered to assess the system's performance. The CBR system integrates soil, crop, and climate variables to provide tailored recommendations for nitrogen, phosphorus, and potassium applications.ResultsThe results revealed that the FRS was deemed acceptable for application in the region, with expert evaluations rating the recommendations based on their experience and knowledge. Additionally, valuable feedback was provided to facilitate future enhancements to the system.DiscussionBased on expert feedback and system performance, the proposed FRS meets the minimum requirements for deployment in real crops, serving as a valuable tool for small-scale farmers. Future work will expand the case base and refine recommender algorithms to improve accuracy and usability.