bluuebunny/crossref_metadata_2025_split huggingface dataset:
The location and development of Replicon Cluster Domains in early replicating DNA
Authors: da Costa-Nunes José A. , Gierlinski Marek , Sasaki Takayo , Haagensen Emma J. , Gilbert David M. , Blow J. Julian 
Published: August 2023

Background: It has been known for many years that in metazoan cells, replication origins are organised into clusters where origins within each cluster fire near-synchronously. Despite clusters being a fundamental organising principle of metazoan DNA replication, the genomic location of origin clusters has not been documented. Methods: We synchronised human U2OS by thymidine block and release followed by L-mimosine block and release to create a population of cells progressing into S phase with a high degree of synchrony. At different times after release into S phase, cells were pulsed with EdU; the EdU-labelled DNA was then pulled down, sequenced and mapped onto the human genome. Results: The early replicating DNA showed features at a range of scales. Wavelet analysis showed that the major feature of the early replicating DNA was at a size of 500 kb, consistent with clusters of replication origins. Over the first two hours of S phase, these Replicon Cluster Domains broadened in width, consistent with their being enlarged by the progression of replication forks at their outer boundaries. The total replication signal associated with each Replicon Cluster Domain varied considerably, and this variation was reproducible and conserved over time. We provide evidence that this variability in replication signal was at least in part caused by Replicon Cluster Domains being activated at different times in different cells in the population. We also provide evidence that adjacent clusters had a statistical preference for being activated in sequence across a group, consistent with the ‘domino’ model of replication focus activation order observed by microscopy. Conclusions: We show that early replicating DNA is organised into Replicon Cluster Domains that behave as expected of replicon clusters observed by DNA fibre analysis. The coordinated activation of different Replicon Cluster Domains can generate the replication timing programme by which the genome is duplicated.
Periprosthetic joint infection after total hip arthroplasty induces histological degeneration of the gluteus medius tendon
Authors: Hedlundh Urban , Karlsson Johanna , Sernert Ninni , Haag Lars , Movin Tomas , Papadogiannakis Nikos , Kartus Jüri 
Published: August 2023

AimsA revision for periprosthetic joint infection (PJI) in total hip arthroplasty (THA) has a major effect on the patient’s quality of life, including walking capacity. The objective of this case control study was to investigate the histological and ultrastructural changes to the gluteus medius tendon (GMED) in patients revised due to a PJI, and to compare it with revision THAs without infection performed using the same lateral approach.MethodsA group of eight patients revised due to a PJI with a previous lateral approach was compared with a group of 21 revised THAs without infection, performed using the same approach. The primary variables of the study were the fibril diameter, as seen in transmission electron microscopy (TEM), and the total degeneration score (TDS), as seen under the light microscope. An analysis of bacteriology, classification of infection, and antibiotic treatment was also performed.ResultsBiopsy samples from the GMED from infected patients revealed a larger fibril diameter than control patients, as seen in the TEM (p < 0.001). Uninfected patients were slightly older and had their revisions performed significantly later than the infected patients. Histologically, samples from infected patients revealed significantly more vascularity (p < 0.001), the presence of glycosaminoglycans (p < 0.001), and a higher TDS (p = 0.003) than the control patients. The majority of patients had staphylococcal infections of various species.ConclusionMore histological degeneration in the GMED was found in patients undergoing THA revision surgery due to PJI than in patients undergoing THA revision surgery due to other reasons.Cite this article: Bone Jt Open 2023;4(8):628–635.
Künstliche Intelligenz zur Entscheidungsunterstützung in Leitstellen des Personennahverkehrs – Technische und sozio-technische Herausforderungen
Authors: Kopp Tobias , Weitemeyer Robin , Beyer Jens , Ziegler Dominic , Hess Roxana 
Published: August 2023

ZusammenfassungIm Kontext der Industrie 5.0 gilt die gezielte Kombination von menschlicher und maschineller Intelligenz – z. B. in Form von Entscheidungsunterstützungssystemen – als besonders vielversprechend. Während eine künstliche Intelligenz (KI) Entscheidungsvorschläge unterbreitet, obliegt im Sinne einer menschenzentrierten Gestaltung den beteiligten Personen die Entscheidungshoheit. Die KI-Vorschläge basieren zumeist auf umfangreichen historischen Datenbeständen, wie sie typischerweise im öffentlichen Personennahverkehr (ÖPNV) anfallen. Diese können bspw. genutzt werden, um Mitarbeitenden in ÖPNV-Leitstellen im Falle unvorhergesehener Störungen geeignete dispositive Maßnahmen wie Umleitungen oder Fahrplanänderungen vorzuschlagen. Dieser anspruchsvolle und oftmals zeitkritische Prozess verursacht eine hohe kognitive Belastung und bietet sich damit für eine KI-basierte Unterstützung an. Praktische Erfahrungen bei der Konzeption einer solchen hybriden Intelligenzlösung und Ergebnisse aus Gesprächen mit betroffenen Mitarbeitenden verdeutlichen allerdings, dass neben technischen auch zahlreiche sozio-technische Herausforderungen an der Mensch-Maschine-Schnittstelle bestehen. Diese betreffen u. a. die Akzeptanz des Systems oder die mangelnde Formalisierbarkeit der intuitiven und individuell unterschiedlichen menschlichen Entscheidungsabläufe. Der Artikel analysiert diese Herausforderungen und trägt damit zu einer realistischeren Bewertung des praktischen Potenzials hybrider Intelligenzlösungen bei.
Nivel Corona Cohort: A description of the cohort and methodology used for combining general practice electronic records with patient reported outcomes to study impact of a COVID-19 infection
Authors: Veldkamp Renee , Hek Karin , van den Hoek Rinske , Schackmann Laura , van Puijenbroek Eugène , van Dijk Liset 
Published: August 2023

Aim
A population-based COVID-19 cohort was set up in the Netherlands to gain comprehensive insight in the short- and long-term effects of COVID-19 in the general population. The present study aims to describe the methodology and infrastructure used to recruit individuals with COVID-19, and the representativeness of the population-based cohort. The second aim was to characterize the population by description of their symptoms and health care usage during the acute COVID-19 phase.

Method
The starting point of the set-up of the cohort was to recruit participants in routinely recorded, general practice electronic health records (EHR) data, which are sent to the Netherlands Institute for Health Services Research Primary Care Database (Nivel-PCD) on a weekly basis. Patients registered with COVID-19 were flagged in the Nivel-PCD based on their COVID-19 diagnoses. Flagged patients were invited for participation by their general practitioner via a trusted third party. Participating patients received a series of four questionnaires over the duration of one year allowing for a combination of data from patient reported outcomes and EHRs.

Results
In this study, results from the first questionnaire are used. The Nivel Corona Cohort consists of 442 participants and is population-based, containing a complete image of severity of symptoms from patients with none or hardly any symptoms to those who were hospitalized due to the COVID-19. The five most prevalent symptoms during the acute COVID-19 phase were fatigue (90.5%), reduced condition (88.2%), coughing/sneezing/stuffy nose (79.3%), headache (75.4%), and myalgia (66.7%).

Conclusion
The population-based Nivel Corona Cohort provides ample opportunities for future studies to gain comprehensive insight in the short- and long-term effects of COVID-19 by combining patients’ perspectives and clinical parameters via the EHRs within a long-term follow-up of the cohort.
Changes in the degree of substitution of HES in vivo and their influence on methods for determining HES concentrations in plasma
Authors: Lukasewitz Peter , Rischer Philipp , Schedel Nina , Stay David , Wulf Hinnerk , Stief Thomas , Volberg Christian 
Published: August 2023

Objective
The degree of substitution (DS) of HES describes the average proportion of substituted glucose molecules in a starch molecule. Although no quantitative studies of the in vivo behavior of the DS have been conducted so far, most pharmacokinetic studies to date have measured HES concentrations using the enzymatic method. This method assumes that at any point in time after an infusion, the DS in a serum remains constant and is identical to the DS in the infused solution. In the present study, we examined the changes in the DS of HES 130/0.42 in vivo in an animal model and compared two methods of measuring HES concentrations in plasma (the enzymatic and the o-Toluidine method).

Methodology
We randomized 22 pigs into 2 groups. After induction of anesthesia, the pigs received 500 ml or 1000 ml of HES 130/0.42 (Tetraspan®). The DS was measured directly after the infusion, then after 30, 60, 120 and 240 minutes. In determining the DS, the hydroxyethyl starch was extracted from the plasma and hydrolyzed with hydrochloric acid to form non-substituted glucose and hydroxyethyl glucose. Subsequently, the concentration of free unsubstituted glucose was determined enzymatically and the total concentration of all (i.e., substituted and unsubstituted) glucose molecules was determined using the o-Toluidine method. From this, the concentration of the substituted glucose (hydroxyethyl glucose) and the DS could be calculated. In addition, the HES concentration was measured first in vitro and then in vivo at any point after the infusion by both the enzymatic method and the o-Toluidine method.

Results
The DS increased significantly directly after the infusion from 0.42 to 0.53 (for 500ml) or to 0.50 (for 1000ml); 4 hours later this had further increased to 0.55 and 0.54, respectively (p <0.0001).
The HES concentration in vitro showed no significant difference (p = 0.17) when determined with the enzymatic and the o-Toluidine method.
In contrast, the serum concentrations in vivo displayed significant differences (p<0.0001) between the two measurement methods. Immediately after the infusion of 500ml HES, the concentration measured with the o-Toluidine method was 31% higher than the one measured with the enzymatic method; 4 hours later, this discrepancy was still at 25%. For 1000 ml HES, the differences amounted to 16% and 25%, respectively.

Conclusion
The DS of HES in vivo increases significantly over time. As a result, an HES concentration measured with the enzymatic method in vivo will be significantly lower than the same concentration determined with the o-Toluidine method.
In future pharmacokinetic studies, HES concentrations should be measured using a method that takes into account changes in the DS in vivo.
Endoscopic Vacuum Therapy for Upper Gastrointestinal Leaks and Perforations: Analysis From a Multicenter Spanish Registry
Authors: Momblan Dulce , Gimeno Garcia Antonio Z. , Busquets David , Juzgado Diego , García Lledó Javier , Ferrero Esther , Tejedor-Tejada Javier , Junquera Félix , Díaz-Tasende José , Moris Maria , Rodriguez de Santiago Enrique , Gornals Joan , Garrido Carmen , Gonzalez-Vazquez Santiago , Guarner-Argente Carlos , Repiso Alejandro , Esteban Jose Miguel , Loras Carme , Seoane Agustín , Fernández-Simon Alejandro , Guevara Henry Cordova , Ibarzabal Ainitze , Morales Xavier , Curell Anna , Cardenas Andrés , Ríos José , de Lacy Antonio Maria , Sendino Oriol 
Published: August 2023

INTRODUCTION:
Endoscopic vacuum therapy (EVT) is a novel technique for closing upper gastrointestinal (UGI) defects. Available literature includes single-center retrospective cohort studies with small sample sizes. Furthermore, evidence about factors associated with EVT failure is scarce. We aimed to assess the efficacy and safety of EVT for the resolution of UGI defects in a multicenter study and to investigate the factors associated with EVT failure and in-hospital mortality.

METHODS:
This is a prospective cohort study in which consecutive EVT procedures for the treatment of UGI defects from 19 Spanish hospitals were recorded in the national registry between November 2018 and March 2022.

RESULTS:
We included 102 patients: 89 with anastomotic leaks and 13 with perforations. Closure of the defect was achieved in 84 cases (82%). A total of 6 patients (5.9%) had adverse events related to the EVT. The in-hospital mortality rate was 12.7%. A total of 6 patients (5.9%) died because of EVT failure and 1 case (0.9%) due to a fatal adverse event. Time from diagnosis of the defect to initiation of EVT was the only independent predictor for EVT failure (odds ratio [OR] 1.03, 95% confidence interval [CI] 1.01–1.05, P = 0.005). EVT failure (OR 24.5, 95% CI 4.5–133, P = 0.001) and development of pneumonia after EVT (OR 246.97, 95% CI 11.15–5,472.58, P = 0.0001) were independent predictors of in-hospital mortality.

DISCUSSION:
EVT is safe and effective in cases of anastomotic leak and perforations of the upper digestive tract. The early use of EVT improves the efficacy of this technique.
Failing or Prevailing? Russian Educational Discourse in the Israeli Academic Classroom
Authors: Zbenovich Claudia 
Published: August 2023

Abstract
This article seeks to explore the ways in which Russian–Soviet educational discourse survives in Israeli universities by bringing its underlying cultural messages to Israeli students. It focuses on the interpretation of educational discursive perceptions and practices of Russian-speaking professors who teach at Israeli universities. The study suggests considering the manifestation of the Russian–Soviet educational scenario through its enforcement in the Russian linguistic repertoire of meta-class talk, in which the professors report on the educational modes of in-class interaction. The analysis reveals that immigrant university teachers adopt communicative strategies imbued with key Russian–Soviet educational messages making them a cultural resource in the Israeli academic context. Concomitantly, however, the professors’ voices seem to be opposed to local Israeli cultural perception and practice. The study therefore seeks to uncover how enacted patterns of educational style reflect the cross-cultural condition of the academic context. It adopts the communicative–pragmatic perspective and focuses on key cultural educational scripts, rhetorical argumentation strategies, and the realization of speech acts in the university interaction.
Constructing a Theoretical System for Chinese Films and Television: A Review of Academic Views of China’s Mainstream Film and Television Since 2017
Authors: Hu Zhifeng , Chen Yin 
Published: August 2023

Abstract
Since 2017, new achievements have been made in the field of mainstream Chinese films and television: specifically, in the interpretation of strategic policies, research on creation and production, and foundational theoretical research. These achievements have chiefly been embodied in the following areas: reaching historical achievements and changes in comprehensive, systematic and in-depth interpretation and analysis of China’s mainstream films and television; focusing on the creative practices, innovations and trends in China’s mainstream films and television; elucidating the remarkable characteristics of development; distilling the Chinese school and Chinese model; proposing a series of original academic discourse; forming a series of important theoretical achievements; and completing the first stage of constructing a system for Chinese films and television in the new era.
Inverse vector problem of diffraction by inhomogeneous body with a piecewise smooth permittivity
Authors: Medvedik Mikhail Y. , Smirnov Yury G. , Tsupak Aleksei A. 
Published: August 2023

Abstract
The vector problem of reconstruction of an unknown permittivity of an inhomogeneous body is considered.
The original problem for Maxwell’s equations with an unknown permittivity and a given permeability is reduced to the system of integro-differential equations.
The solution to the inverse problem is obtained in two steps.
First, a solution to the vector integro-differential equation of the first kind is obtained from the given near-field data.
The uniqueness of the solution to the integro-differential equation of the first kind is proved in the classes of piecewise constant functions.
Second, the sought-for permittivity is straightforwardly calculated from the found solution and the total electric field.
A series of test problems was solved using the two-step method.
Procedures of approximate solutions’ refining were implemented.
Comparison between the given permittivities and the found approximate solutions shows efficiency of the proposed method.
On the convergence, stability and data dependence results of the JK iteration process in Banach spaces
Authors: Ullah Kifayat , Saleem Naeem , Bilal Hazrat , Ahmad Junaid , Ibrar Muhammad , Jarad Fahd 
Published: August 2023

Abstract
This article analyzes the JK iteration process with the class of mappings that are essentially endowed with a condition called condition (E). The convergence of the iteration toward a fixed point of a specific mapping satisfying the condition (E) is obtained under some possible mild assumptions. It is worth mentioning that the iteration process JK converges better toward a fixed point compared to some prominent iteration processes in the literature. This fact is confirmed by a numerical example. Furthermore, it has been shown that the iterative scheme JK is stable in the setting of generalized contraction. The data dependence result is also established. Our results are new in the iteration theory and extend some recently announced results of the literature.
L’agentivité collective déployée lors de l’implication d’étudiantes au comité de programme du doctorat réseau en éducation
Authors: Nadeau-Tremblay Sophie , Ouellet Cassandre , St-Jacques Caroline 
Published: August 2023

Dans le cadre de la révision du programme du doctorat réseau en éducation, les besoins et les moyens de soutien aux étudiants doctorants ont été identifiés comme centraux dans la poursuite du cheminement scolaire. Appuyées des recommandations du rapport d’autoévaluation et de documents externes, des personnes professeures et trois étudiantes ont proposé une enquête à l’ensemble des corps étudiant et professoral. L’article vise à décrire le processus méthodologique utilisé ainsi que la portée de la démarche dans la formation doctorale des étudiantes y ayant contribué en prenant appui sur le concept d’agentivité collective (Bandura, 2001 ; Brennan, 2012). Une carte conceptuelle des manifestations d’agentivité collective dans le cadre d’une implication étudiante est proposée ainsi que les questionnaires utilisés pour la démarche de consultation qui pourront soutenir la réflexion d’équipes de personnes professeures et d’étudiantes dans le soutien à offrir à ces derniers.ères. La persistance aux études pourrait s’en trouver bonifiée.
Démarche et outils d’accompagnement à l’intention des personnes conseillères pédagogiques pour soutenir l’élaboration collaborative d’un programme d’insertion professionnelle au sein d’une équipe-école
Authors: Lussier Mireille , Guay Marie-Hélène 
Published: August 2023

Comment accompagner le développement d’un programme d’insertion professionnelle au sein d’une équipe-école ? Cet article s’adresse aux personnes conseillères pédagogiques qui se posent cette question. Nous présentons une démarche et des outils d’accompagnement, appuyés théoriquement, pour soutenir l’élaboration et la mise en œuvre collaboratives d’un programme d’insertion professionnelle avec et pour les membres d’une équipe-école. Les personnes conseillères pédagogiques pourront ainsi planifier un soutien collaboratif, différencié et rigoureux du personnel novice accueilli dans les établissements scolaires du Québec.
1835 Yılında Karaferye Kazasının Nüfus ve Demografik Yapısı
Authors: MALKADI ÇİÇEK Burcu 
Published: August 2023

Osmanlı döneminde Selânik Vilâyetine bağlı olan ve etnik, dini ve kültürel çeşitliliği bakımından Rumeli’nin en renkli kazalarından biri olan Karaferye, Balkan Savaşlarına kadar Türk egemenliğinde kalmıştır. 1826’da Yeniçeri Ocağı kaldırılmış ve yerine Asâkir-i Mansûre-i Muhammediye ordusu teşkil edilmiştir. Bu ordunun nefer ihtiyacının karşılanması için Anadolu ve Rumeli’de 1830-1831 yılları arasında sadece erkekleri kapsayan nüfus sayımları yapılmıştır. Reayanın kalabalık olduğu yerlerde nüfus, etnik bakımdan bölümlere ayrılarak kayda geçirilmiştir. Karaferye kazasının erkek nüfusu yerli-yabancı Müslim- Gayrimüslim, Kıptî ve Ulah nüfus olarak ayrı ayrı kaydedilmiştir. Buna göre Karaferye’nin erkek nüfusu 12.732 olarak tespit edilmiştir.
Biz bu çalışmamızda hem ilk sayımdan sonra şehirdeki nüfus hareketliliğini görebilmek hem de nüfusun etnik-dinsel bileşenlerine ilişkin daha ayrıntılı veriler içermesi bakımından 1835 yılına ait 4934, 4935, 4936 ve 4937 numaralı defterinden hareketle Karaferye kazasının nüfus ve demografik yapısını ortaya koymaya çalışacağız. Bu defterlerde Karaferye’de yaşayan Türk ve Rum nüfus herhangi etnik bir vurgu yapılmaksızın Müslim ve Gayrimüslim nüfus şeklinde kaydedilirken Yahudi, Arnavut, Bulgar, Ulah ve Kıptî nüfus ayrı ayrı belirtilmiştir. 1835 yılında şehirde 29 mahalle ve 115 köyde 10.984 erkek nüfus kaydedilmiştir. Bu rakamlara yabancı reaya dâhil değildir. Karaferye kazasında nüfusun %67,70’i kırsalda yaşamaktadır. Veriler Karaferye kazasında şehirli nüfus oranının diğer Osmanlı şehirlerinden oldukça yüksek olduğunu göstermektedir.
El viaje de la filosofía por los caminos de la traducción
Authors: Tardáguila Esperanza 
Published: August 2023

Este artículo presenta a la Filosofía como viajera constante a través de toda su historia junto con su fiel compañera, la traducción, la cual ha permitido al conocimiento filosófico en particular y al conocimiento en general; partir de algún lugar para llegar a otro donde siempre es recibido, asimilado y transformado en nuevo conocimiento.
Traducción y tradición: ¿ruptura?
Authors: Sánchez Segura Luz Adriana 
Published: August 2023

El objetivo central es proponer un concepto histórico-filosófico-cultural denominado el sabotaje, en tanto instrumento de resistencia cultural y perspectiva crítica de interpretación filosófica respecto de los fenómenos culturales y de la situación histórica de América colonial (siglos XVI - XVIII). El sabotaje desarrolla un aparato teórico capaz de otorgar la operatividad a una perspectiva de interpretación filosófica que implica una lectura crítica y propositiva en el ámbito de la traducción y la mediación cultural presente en las obras de Hernando de Alvarado Tezozómoc: Crónica Mexicana (1598), Crónica Mexicáyotl (1609) y de Felipe Guamán Poma de Ayala: Primer Nueva Corónica y Buen Gobierno (1615/1616). Estas obras funcionan en tanto textos culturales como ejemplos de la configuración político-filosófica e histórico-cultural que inaugura una reflexión hermenéutica americana. Esto significa que nuestra investigación busca demostrar la ejecución de la perspectiva de la resistencia cultural en América colonial y, a su vez, consolida la resignificación del sabotaje como un vehículo conceptual original y explicativo de las posibilidades de la reflexión filosófica desde América colonial.