bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction and validation of a prognostic risk model for cuproptosis-related lncrna in breast cancer
Authors: Chen Chenxi , Zhong Hangtian , Chen Lihua , Yu Weihua , Liu Li 
Published: February 2023

Abstract
Background
Long non-coding RNAs(LncRNAs) are vital in regulating programmed death in breast cancer. Cuproptosis is a newly type of regulatory cell death(RCD) in tumor pathogenesis, development, prognosis and potentially as a target for immunotherapy.
Methods
The expression profile data and clinical information data of breast cancer (BRCA) from the Cancer Genome Atlas (TCGA) were downloaded using the R package TCGA biolinks. The differential expression analysis was performed using R package ‘limma’. Univariate Cox analysis was performed on deregulated LncRNA related to cuproptosis to screen signature related to the prognosis of breast cancer. Using Cytoscape construct mRNA (cuproptosis-related factors) and co-expression of LncRNA networks. The network was verified in TCGA training set, global set and test set. Functional enrichment analysis was also performed for studied pathway.
Results
In this study, 1156 samples was screened from TCGA dataset for differential expression analysis, and finally a total of 284 dysregulated LncRNAs in breast cancer was identified. Then based on the expression of cuproptosis factors and dysregulated LncRNAs, a total of 140 cuproptosis-related dysregulated lncRNAs (CRDLs) were obtained by correlation analysis. Finally, eight model genes(LINC01235, MIR205HG, RP11-459E5.1, RP11-817J15.3, KLHDC7B-DT, LMNTD2-AS1, RP11-6O2.3, LINC00987) were obtained, one of which was a risk factor., we identified a prognostic risk model of dysregulated lncRNAs associated with cuproptosis by collecting RNA-seq data from TCGA. Subsequently, we verify this model, and the results showed that there were significant differences between the high and low-risk groups. Then, we took the test set and whole set, respectively, to perform model verification and prove that they have stable and favorable performance on survival prediction. And the Risk score we selected can be used as an independent prognostic factor. There were significant differences in the proportion of immune cell infiltration, genomic mutations, pathway enrichment scores, expression levels of immune checkpoints and chemoresistance between high and low-risk groups.
Conclusion
Ultimately, we conclude that the risk score can predict the benefit of immunotherapy in patients, and the model genes may be markers of immunotherapy response.
Metabolic programming determines the pathogenicity of　Campylobacter jejuni
Authors: Hosomi Koji , Hatanaka Noritoshi , Hinenoya Atsushi , Tojima Yoko , Furuta Mari , Nagatake Takahiro , Saika Azusa , Kawai Soichiro , Yoshii Ken , Kondo Saki , Yamasaki Shinji , Kunisawa Jun 
Published: February 2023

AbstractMany pathogens acquire pathogenicity through a specialized capacity for metabolic adaptation, but unique adaptations in the metabolism ofCampylobacter jejuniremain to be investigated. Here, we found that cultivation ofC. jejuniunder different conditions resulted in changes to the metabolic pathways associated with their altered pathogenicity in mice, such as highly frequent colonization and subsequent severe inflammation in the intestine. The metabolic changes involved the aspartate transferase–mediated pathway with serine as a substrate, which was required for aerobic energy generation. Our findings propose a novel pathway for controlling the pathogenicity ofC. jejuniwith promising preventive and therapeutic strategies for targeting bacterial metabolism.
Effect of scanning pathways on trueness and precision in full-arch optical impression
Authors: Kuroda Shota , Yotsuya Mamoru , Sato Toru , Hisanaga Ryuichi , Nomoto Syuntaro , Sekine Hideshi 
Published: February 2023

Abstract
Background: In this study, we investigate the effect of differences in scanning pathway during optical impression on the trueness and precision of full-arch impressions.
Methods: Reference data were obtained using a lab scanner. All the optical impressions were measured across the dental arch using TRIOS® 3 in four different pathways. The reference data and optical impression data were superimposed using a best-fit method. The criteria for superimposition included the center of one starting side dental arch (partial-arch best-fit method : PB) and the center of full-arch (full-arch best fit method : FB). Data were compared between the left and right molars (starting and ending sides). The mean values (scan deviations) of trueness (n = 5) and precision (n = 10) were calculated after the difference in deviation between each group was absolutized. Visual observations using the superimposed color map images showed the variation in trueness.
Results: There was no significant difference in scanning time or the amount of scan data between the four scanning pathways studied. Trueness did not differ significantly among the four pathways with respect to starting and ending sides, regardless of superimposition criteria. Precision with PB was significantly different between scanning pathways A and B, between pathways B and C for the starting sides and between scanning pathways A and B, between pathways A and D for the ending sides. In contrast, there was no significant difference between starting sides and ending sides pathways for FB. For PB, color map images showed a large error range in the direction toward the molar radius for occlusal surface and cervical regions on the ending sides.
Conclusion: Differences in scanning pathways did not affect trueness, regardless of superimposition criteria. Scan pathway B on the starting side and scan pathway D on the ending side were the most accurate, and the difference in scanning pathways was observed to affect the precision of PB.
CRISPR-Cas9 RAG2 Correction via Coding Sequence Replacement to Preserve Endogenous Gene Regulation and Locus Structure
Authors: Allen Daniel , Knop Orli , Itkowitz Bryan , Iancu Ortal , Beider Katia , Lee Yu Nee , Nagler Arnon , Somech Raz , Hendel Ayal 
Published: February 2023

Abstract
RAG2-SCID is a primary immunodeficiency caused by mutations in Recombination-activating gene 2 (RAG2), a gene intimately involved in the process of lymphocyte maturation and function. ex-vivo manipulation of a patient’s own hematopoietic stem and progenitor cells (HSPCs) using CRISPR-Cas9/rAAV6 gene editing could provide a therapeutic alternative to the only current treatment, allogeneic hematopoietic stem cell transplantation (HSCT). Here we show a first-of-its-kind RAG2 correction strategy that replaces the entire endogenous coding sequence (CDS) to preserve the critical endogenous spatiotemporal gene regulation and locus architecture. Expression of the corrective transgene led to successful development into CD3+TCRαβ+ and CD3+TCRγδ+ T cells and promoted the establishment of highly diverse TRB and TRG repertoires in an in-vitro T-cell differentiation platform. We believe that a CDS replacement technique to correct tightly regulated genes, like RAG2, while maintaining critical regulatory elements and conserving the locus structure could bring safer gene therapy techniques closer to the clinic.
Gut microbes predominantly act as symbiotic partners rather than raw nutrients
Authors: Soares Nuno Felipe da Silva , Quagliariello Andrea , Yigitturk Seren , Martino Maria Elena 
Published: February 2023

Abstract
Animals and their gut microbes mutually benefit their health. In this frame, nutrition has a central role by directly affecting both host and microbes’ fitness and their effects. This makes nutritional symbioses a complex and dynamic tri-system of diet-microbiota-host. Despite recent discoveries on this field, full control over the interplay among these partners is challenging and hinders the resolution of fundamental questions, such as how to parse the gut microbes’ effect as raw nutrition or as symbiotic partners? To tackle this, we made use of the well-characterized Drosophila melanogaster/Lactiplantibacillus plantarum experimental model of nutritional symbiosis to generate a quantitative framework of gut microbes’ effect on the host. We show that the beneficial effect of L. plantarum strains primarily results from the active relationship as symbionts rather than raw nutrients, regardless of the bacterial strain. Metabolomic analysis of both active and inactive bacterial cells further demonstrated the crucial role of the production of beneficial bacterial metabolites, such as N-acetylated-amino-acids, as result of active bacterial growth and function. Altogether, our results provide a ranking and quantification of the main bacterial features contributing to sustain animal growth. We demonstrate that viability is the predominant and necessary variable involved in bacteria-mediated benefit, followed by strain-specific properties and the nutritional potential of the bacterial cells as direct energy source. This contributes to elucidate the role of beneficial bacteria and probiotics, creating a broad quantitative framework for host-gut microbiome that can be expanded to other model systems.
Enabling low-drift flexible perovskite photodetectors by electrical modulation for wearable health monitoring and weak light imaging
Authors: Tang Yingjie , Jin Peng , Wang Yan , Li Dingwei , Chen Yitong , Ran Peng , Fan Wei , Liang Kun , Ren Huihui , Xu Xuehui , Wang Rui , Yang Yang , Zhu Bowen 
Published: February 2023

Abstract
Metal halide perovskites (MHPs) are promising for next-generation flexible photodetectors (FPDs) owing to their low-temperature solution processability, mechanical flexibility, and excellent photoelectric properties. However, the defects and notorious ion migration in polycrystalline MHPs often lead to high and unstable dark current, thus deteriorating their detection limit and long-term operations. Here, we propose an electrical field modulation strategy to significantly reduce the dark current of MHPs-based FPD more than 1000 times (from ~ 5 nA to ~ 5 pA). Meanwhile, ion migration in MHPs was effectively suppressed, and the MHPs-based FPD shows a long-term continuous operational stability (~ 8000 s) with low signal drift (~ 4.2 × 10− 4 pA per second) and ultralow dark current drift (~ 1.3 × 10− 5 pA per second). Benefitting from the electrical modulation strategy, a high signal-to-noise ratio (SNR) wearable photoplethysmography (PPG) sensor and an active-matrix photodetector array for weak light imaging are successfully demonstrated. This work offers a universal strategy to improve the performance of MHPs for wearable FPD and flexible image sensor applications.
Ucp4 deficiency of cerebellar Purkinje cells induces bradykinesia
Authors: Wang Ya-Yun , Liu Hui , Li Shu-Jiao , Feng Ban , Huang Yun-Qiang , Liu Shui-Bing , Yang Yan-Ling 
Published: February 2023

Abstract
Although uncoupling protein 4 (UCP4) is the most abundant protein reported in the brain, the biological function of UCP4 in cerebellum and pathological outcome of UCP4 deficiency in cerebellum remain obscure. To evaluate the role of Ucp4 in the cerebellar Purkinje cells (PCs), we generated conditional knock-out of Ucp4 in PCs (Pcp2cre;Ucp4fl/fl mice) by breeding Ucp4fl/fl mice with Pcp2cre mice. PCR, western blot, double immunofluorescent staining and triple RNAscope in situ hybridization confirmed the specific ablation of Ucp4 in PCs in Pcp2cre;Ucp4fl/fl mice, and the reservation of Ucp2, the analog of Ucp4. Open field test, CatWalk analysis, rotarod and elevated plus-maze tests showed that Pcp2cre;Ucp4fl/fl mice displayed a characteristic bradykinesia in the spontaneous movements, and such bradykinesia disorder was not deteriorated in harmaline-induced tremor application. The electromyogram recordings detection on gastrocnemius muscle excluded the possibility of hypotonia in Pcp2cre;Ucp4fl/fl mice. And the electrical patch clamp recordings showed the altered properties of both spontaneous and evoked firings in PCs of Pcp2cre;Ucp4fl/fl mice. Also, the knockdown of Ucp4 significantly increased reactive oxygen species generation in the cerebellum. The present study is the first to report a close relationship between UCP4 deletion with PCs impairment, and suggests the importance of UCP4 in the substantial support of mitochondrial function homeostasis in bradykinesia. UCP4 might be a therapeutic target for the cerebela-related movement disorder.
Relationship of Anxiety and Depression with Psychological Flexibility and Sleep Quality in Women with Breast and Gynecologic Cancer: A Structural Equation Modeling Analysis
Authors: Kiyak Sibel , Polat Hilal Türkben 
Published: February 2023

Abstract
Objective
Being diagnosed with cancer and receiving long-term cancer treatments can affect the mental health of the patients. The current study aimed to evaluate psychological flexibility and sleep quality as markers of anxiety and depression in patients with breast and gynecologic cancer.
Methods
A descriptive-relational study. This study was conducted with 212 patients with breast and gynecological cancers in the day treatment unit of an oncology department of a medical facility of a state university. Data were collected with participant information form, Hospital Anxiety and Depression Scale, Psychological Flexibility Scale, and Pittsburgh Sleep Quality Index.
Results
Of the studied patients, 36.3% and 55.3% were in the risk group for anxiety and depression, respectively. Furthermore, 75.3% of participants had poor sleep quality and medium–high psychological flexibility. While psychological flexibility has a positive effect on anxiety and depression, poor sleep quality and high body mass index have a negative effect. Psychological flexibility, sleep quality, and body mass index explained 27% variance in anxiety and depression levels.
Conclusions
Poor sleep quality, low psychological flexibility, and high body mass index negatively affected anxiety and depression levels in patients.
A Study on the Nostalgia effect of Dance for All Participants during the COVID-19 Pandemic
Authors: An Jung-Hun , Lim Sun-Young , Lee Ji-Seol 
Published: February 2023

Abstract
Background: The purpose of this study is to examine the effect of participants' nostalgia for the before COVID-19 DFA experience on their future participation continuation intention. To achieve this purpose, nostalgia, cognitive emotion regulation (CER), coping flexibility (CF), resilience, and participation continuance intention (PCI) were set as major variables and the structural influence relationship between them was explored.
Methods: A survey was con-ducted on 557 “dance for all” participants (DFAP’s) who joined public sports facilities, academies, and clubs in Seoul and Gyeonggi Province in South Korea. Data analysis was performed on Windows PC/SPSS 26.0 and AMOS 24.0 ver. frequency analysis, correlation analysis, confirmatory factor analysis and structural equation modeling were used to analyze the survey results.
Results: First, all sub-factors of the nostalgia of DFAP’s has a statistically significant effect on CER. Second, CER of DFAP's has a statistically significant effect on CF. Third, CER of DFAP's does not have a statistically significant effect on resilience. Fourth, CF and resilience of DFAP's has a statistically significant effect on PCI.
Conclusions: Therefore, this study’s novelty is that it provides practical implications for the application of nostalgia as an alternative to satisfying the desire to participate in physical activities such as DFA during the “post COVID-19”era.
Palliative Care Needs Among Jordanian Women Living with Stroke: A Descriptive Phenomenological Study
Authors: Alhalabi Marwa Nayef , Khalaf Inaam Abdulla , Zeilani Ruqayya Sayed , Bawadi Hala Ahmad , Musa Ahmad S. , Nashwan Abdulqadir J. 
Published: February 2023

Abstract
Background: Stroke is a common neurological disease. Women with stroke suffer many physical, psychosocial, and spiritual needs. Consequently, women living with stroke may have many marginalized palliative care needs as perceived by health care providers. However, the experience of women living with stroke and palliative care needs is understudied in Jordan.
Aim: This study aimed to explore the palliative care needs among women living with stroke in Jordan.
Methods: A qualitative descriptive phenomenological approach was employed with a purposive sample recruited from twelve women living with stroke Data were collected using face-to-face semi-structured interviews. The Colaizzi (1978) method was used for data analysis.
Results: The study revealed three main emerging themes that reflected the palliative care needs of women living with stroke, which are: living with bothersome symptoms, receiving bad news, and spiritual practices, beliefs, and needs.
Discussion: This study sheds light on the aspects of Jordanian women with a comprehensive view of all aspects of life after suffering a stroke. The findings suggest that the burden of stroke has far-reaching consequences for women's lives. They have many unmet palliative needs that must be considered in the treatment plan.
Conclusion: This study provides insight into the palliative care needs of women. Women's health care needs included physical, psychosocial, and spiritual aspects. In line with these needs, integrating the palliative care approach with the rehabilitation program should be considered to improve the quality of life and alleviate the pain and further complications after stroke.
Trust of patients and families in mental healthcare providers and institutions: A cross-cultural study in Chennai, India and Montreal, Canada
Authors: Xavier Salome M. , Malla Ashok , Mohan Greeshma , Mustafa Sally , Padmavati Ramachandran , Rangaswamy Thara , Joober Ridha , Schmitz Norbert , Margolese Howard C. , Iyer Srividya N. 
Published: February 2023

Abstract
Purpose: Cross-cultural psychosis research has typically focused on a limited number of outcomes (generally symptom-related). It is unknown if the purported superior outcomes for psychosis in some low- and middle-income countries extend to fundamental treatment processes like trust. Addressing this gap, we studied two similar first-episode psychosis programs in Montreal, Canada and Chennai, India. We hypothesized higher trust in healthcare institutions and providers among patients and families in Chennai at baseline and over follow-up.
Methods: Upon treatment entry and at months 3, 12 and 24, trust in healthcare providers was measured using the Wake Forest Trust scale and trust in the healthcare and mental healthcare systems using two single items. Non-parametric tests were performed to compare trust levels across sites and mixed-effects linear regression models to investigate predictors of trust in healthcare providers.
Results: The study included 333 patients (Montreal=165, Chennai=168) and 324 family members (Montreal=128, Chennai=168). Across all timepoints, Chennai patients and families had higher trust in healthcare providers and the healthcare and mental healthcare systems. The effect of site on trust in healthcare providers was significant after controlling for sociodemographic characteristics known to impact trust. Patients' trust in doctors increased over follow-up.
Conclusion: This study uniquely focuses on trust as an outcome in psychosis, via a comparative longitudinal analysis of different trust dimensions and predictors, across two geographical settings. The consistent differences in trust levels between sites may be attributable to local cultural values and institutional structures and processes and underpin cross-cultural variations in treatment engagement and outcomes.
Complex Kraenkel-Manna-Merle system in a ferrite: N-fold Darboux transformation, generalized Darboux transformations and solitons
Authors: Shen Yuan , Tian Bo , Zhou Tian-Yu , Cheng Chong-Dong 
Published: February 2023

Abstract
Ferromagnetic materials such as the ferrites are used in the electronic and energy industries. Here, we concentrate on a complex Kraenkel-Manna-Merle system for the ultra-short waves in a ferrite. An N-fold Darboux transformation of that system is presented via an existing Lax pair, where N is a positive integer. A generalized (1, N − 1)- fold Darboux transformation, which admits one spectral parameter, and a generalized (m, N − m)-fold Darboux transformation, which admits m spectral parameters, are proposed based on that N-fold Darboux transformation, where m is a positive integer. One-, two- and three-soliton solutions of that system are determined via that N-fold Darboux transformation. Double-pole and triple-pole soliton solutions of that system are derived via that (1, N − 1)-fold Darboux transformation. Those solitons are graph- ically represented for the magnetization and external magnetic field related to a ferrite.
Potential impact of obesity on the aggressiveness of low-to intermediate-risk papillary thyroid carcinoma: results from a MASTER cohort study
Authors: Kim Mijin , Kang Yae Eun , Park Young Joo , Koo Bon Seok , Ku Eu Jeong , Choi June Young , Lee Eun Kyung , Kim Bo Hyun 
Published: February 2023

Abstract
Purpose: Obesity is associated with an increased risk of papillary thyroid carcinoma (PTC). Evidence of the impact of obesity on PTC aggressiveness is scarce. We aimed to evaluate the association between the body mass index (BMI) and the presence of aggressive features of low- to intermediate-risk PTC in a prospective cohort.
Methods: A total of 1,088 patients with low- to intermediate-risk PTC who underwent lobectomy at 22 hospitals in Korea were prospectively enrolled and divided into three groups according to BMI, as follows: normal/underweight (<23 kg/m2), overweight (23–24.9 kg/m2), and obese (≥25 kg/m2). Clinicopathological features of PTC at diagnosis were evaluated.
Results: Obese patients had a larger primary tumor size, higher rate of macro-PTC (>1 cm), and greater incidence of extra-thyroidal extension (ETE) and vascular invasion than those who were not classified as obese. Increased BMI was positively associated with the incidence of macro-PTC (p for trend = 0.019), ETE (p for trend = 0.013), and vascular invasion (p for trend = 0.021). After adjusting for age, sex, pathological features, metabolic syndrome, and thyroid function test, obesity was a risk factor for ETE (odds ratio = 1.6, 95% confidence interval: 1.1–2.3, p = 0.007) in women. This association was significant regardless of whether or not the women had metabolic syndrome. There was no significant association between obesity and aggressive PTC features in men.
Conclusion: These results suggest that BMI at the time of thyroid cancer diagnosis may affect the aggressiveness of low- to intermediate-risk PTC, especially in women.