bluuebunny/crossref_metadata_2025_split huggingface dataset:
Education Research in Orthopaedic Surgery
Authors: Martinez Victor H. , Zaheer Aroob , McCarrell Jerod , Checketts Jake X. , Hanson Chad D. 
Published: April 2023

Introduction:
With the ever-changing dynamics of surgical training, it is essential for the content of educational research to evolve simultaneously. This study aimed to assess the current state of scholarly work in orthopaedic training education and to identify particular educational topics that are trending in the literature.

Methods:
A PubMed search string was used to identify literature on orthopaedic surgery education from 2016 to 2021. Publications on orthopaedic surgery education were divided into 8 education-based categories: (1) operative training and simulation, (2) curriculum and career development, (3) diversity, (4) wellness and burnout, (5) program evaluation and match data, (6) trainee assessments, (7) social media, and (8) other. Univariate analysis and linear regression were used to determine trends.

Results:
A total of 383 orthopaedic surgery education publications met inclusion criteria. Orthopaedic surgery education publications accounted for 0.47% of the total publications from all 54 journals. Fifteen journals provided 85% of all orthopaedic education publications (N = 325). The Journal of Bone and Joint Surgery produced the most orthopaedic surgery education publications (76, 20%), followed by the Journal of Surgical Education (62, 16%) and the Journal of the American Academy of Orthopaedic Surgeons (58, 15%). Publications on curriculum and career development were the most common (21.9%). The following most frequent topics were program evaluation and match data (20.9%) and operative training and simulation (18%), respectively. Over the study period, the total amount of orthopaedic surgery education articles increased significantly (R2 = 0.86, p = 0.008). Articles included all levels of training, with resident education accounting for the majority, with 269 articles (70.2%).

Conclusion:
Recent trends in orthopaedic literature indicate an increased emphasis on education as the number of publications has increased, but are still sparse in orthopaedic literature. As the field of orthopaedics continues to advance, we recommend that academic institutions and organizations continue to evaluate and promote evidence-based educational research to ensure that orthopaedic education meets the ever-increasing demands that residents and practicing surgeons will face in the present and future.

Level of Evidence:
III
Augmented Reality Surgical Navigation in Minimally Invasive Spine Surgery: A Preclinical Study
Authors: Huang Xin , Liu Xiaoguang , Zhu Bin , Hou Xiangyu , Hai Bao , Li Shuiqing , Yu Dongfang , Zheng Wenhao , Li Ranyang , Pan Junjun , Yao Youjie , Dai Zailin , Zeng Haijun 
Published: April 2023

Abstract
Background: Augmented reality (AR) is a technology that combines virtual information with the real-world environment. In the medical field, AR technology can be used to show information inside the body.
Methods: We combined augmented reality, artificial intelligence and optical tracking to enhance the augmented reality minimally invasive spine surgery (AR-MISS) system. The system has three functions: augmented reality radiograph superimposition, AR real-time puncture needle tracking and AR intraoperative navigation. The three functions of the system were preliminarily evaluated through beagle animal experiments.
Results: The AR radiographs were successfully superimposed on the real intraoperative videos. The anteroposterior and lateral errors of superimposed AR radiographs were 0.74 ± 0.21 mm and 1.13 ± 0.40 mm, respectively. The puncture needles could be tracked by the AR-MISS system in real time. The anteroposterior and lateral errors of the real-time AR needle tracking were 1.26 ±0.20 mm and 1.22 ± 0.25 mm, respectively. With the help of AR radiographsand AR puncture needles, the puncture procedure could be guided visually by the system in real time. The anteroposterior and lateral errors of AR-guided puncture were 2.47 ±0.86 mm and 2.85± 1.17 mm, respectively.
Conclusion: Theresults indicate that the AR-MISS system is accurate and feasible in a beagle model. Additional clinical assessment is needed.
Huazhuojiedu Decoction Regulates Mitophagy to Alleviate Precancerous Lesions of Gastric Cancer via Sirt3/Foxo3a/Parkin Pathway
Authors: Yang Tianxiao , zhou pingping , Zhang ziwei , hao xinyu , liu shaowei , chen haoyu , Wang yuhua , wang yangang 
Published: April 2023

Abstract
Backgroud：Huazhuojiedu decoction (HZJD) has been demonstrated to be effective in the treatment of precancerous lesions of gastric cancer (PLGC). We aimed to explore the potential mechanisms of HZJD for alleviating PLGC in vivo and in vitro.
Methods: The PLGC rat model was established by administrating 1-Methyl-3-nitro-1-nitrosoguanidine (MNNG) and sodium for 24 weeks, followed by 10 weeks of HZJD decoction or vitamin B12 therapy. The PLGC cell model (MC) was prepared by inducing human gastric mucosal epithelial cells (GES-1) with MNNG. HZJD decoction and vitamin B12 drug-containing serum were given to treat MC cells, meanwhile sirt3 siRNA was transfected into MC cells. The CCK-8 assay and the EdU assay were used to detect cell proliferation. The histopathological changes of gastric tissues were observed by H&E staining, HID/AB staining and AB/PAS staining. The mRNA and protein expressions of on mitophagy-related molecules were detected by RT-qPCR and Western blot. Immunohistochemistry was used to test the differential expressions of sirt3/foxo3a/parkin pathway. Immunofluorescence was used to evaluate mitophagic level. Transmission electron microscopy was used to monitor the degree of mitochondrial damage and the occurrence of mitophagy.
Results: The results indicated that HZJD could retard the pathological progress of gastric mucosa in PLGC rats and reduce the elevated cell proliferation in MC cells. The treatment of HZJD could significantly increase the gene and protein expressions of sirt3, foxo3a, parkin, LC3Ⅱ/Ⅰ, meanwhile decrease the mRNA and protein expressions of p62, tomm20. The colocalization of LC3 and COX Ⅳ was inhibited in PLGC rats, besides the fluorescent intensity of mitophagy was weakened in MC cells. This downtrend of mitophagic level in vivo and in vitro could be reversed by HZJD. More importantly, the improvement of mitophagy by HZJD was associated with sirt3/foxo3a/parkin pathway.
Conclusions: Our results suggested that HZJD decoction could ameliorate PLGC in vivo and in vitro, and its therapeutic effect might be related to regulating mitophagy via sirt3/foxo3a/parkin pathway.
Willingness to receive COVID-19 vaccine and associated factors among students of higher education institutions: the case of Rift Valley University, Adama, Central Ethiopia: a cross sectional study
Authors: Mandefro Endashaw 
Published: April 2023

Abstract
Introduction: The accelerated global spread of COVID-19 and its high death rate have prompted the World Health Organization to implement vaccination as an effective way to curb the spread of the disease. Accordingly, Ethiopia has launched a national vaccination campaign against the disease. However, the willingness of people to receive the vaccine was considered as an important pre-requisite for the success of the vaccination campaign. The aim of this study was to assess the magnitude of willingness towards COVID-19 vaccination and associated factors among students at Rift Valley University, Adama, Ethiopia, in 2022.
Methods: A cross-sectional study was conducted from January 10 to January 25, 2022 among 490 randomly selected students at Rift Valley University, Adama campus. Data was collected by using an interviewer administered questionnaire and was entered into Epi Info version 7 and exported to SPSS version 21 for cleaning and analysis.
Results: The proportion of willingness to receive the COVID-19 vaccine was 60.2% (95%CI: 55.9, 64.5). Being male (AOR: 2.72; 95% CI: 1.14, 2.76), having a family history of COVID-19 infection (AOR: 1.2, 95% CI: 1.07, 1.54), having ever taken any vaccine (AOR: 1.7, 95% CI: 1.14, 2.27), and respondents believing the COVID-19 vaccine has no severe side effects (AOR: 2.3, 95% CI: 1.27, 3.01) were all positive predictors of willingness.
Conclusions: The proportion of willingness to get the COVID-19 vaccine among students of higher education institution in Adama town was far lower than that of the nationwide expected result. Male sex, having a family history of COVID-19 infection, having ever taken any vaccine, and the thought of respondents that the COVID-19 vaccine has no severe side effects were independent predictors of willingness to receive the vaccine.
Recalibration of Framingham Risk for a local population of Sri Lanka
Authors: Ranasinghe Sameera Upashantha , Ekanayake E M D S , Ranasinghe Laksitha Iroshan , Tennakoon Sampath U.B 
Published: April 2023

Abstract
Background
Cardiovascular Diseases (CVD) account for highest number of deaths and disability globally and within Sri Lanka. A CVD risk prediction tool is a simple mean of early identification of high-risk groups which is a cost-effective preventive strategy especially for resource poor countries. Distribution of risk factor levels vary in different regions even within the same country, thus a common risk estimation tool for the country may give false local predictions. Since there’s few published data related to Sri Lanka the aim of this study was to recalibrate the Framingham equation according to the local risk factor profile of a population in Kurunegala region in Sri Lanka.
Method
A cross sectional study was conducted with the participation of 1 102 persons from Kurunegala Regional Director of Health Services area and the data was collectedusing an interviewer administered questionnaire, anthropometric, blood pressure and biochemical measurements.
Results
Original and recalibrated Framingham CVD risk scores predicted 55.5% (N = 612) and 62.3% (N=687) to be having less than 10% CVD risk respectively. Further, the original and recalibrated CVD Risk Scores predicted 2.2% (N = 24) and 1.8% (N=20) to be having CVD risk more than 40% respectively.
Conclusion
These findings show an over prediction of the CVD risk with the original Framingham risk calculations which signifies the importance of development of a region-specific risk prediction tool using local risk factor data in Sri Lanka whichwill prevent unnecessary expenditure to manage people without risk of CVD.
The effect of ultrasound-guided lung recruitment maneuvers on perioperative atelectasis for adults undergoing general anesthesia:A meta-analysis
Authors: Li Shuji , Zhang Shuang , Yin Hong , Yi Mingliang 
Published: April 2023

Abstract
Background:
To systematically evaluate the effects of ultrasound-guided lung recruitment maneuvers(U-LRM) on he incidence of perioperative atelectasis and the Lung ultrasound scores(LUS) with adults undergoing general anesthesia.
Materials and methods
we searched relevant studies in CNKI,Wanfang,CBM,PubMed, EMBASE, and the Cochrane Library up to December ,2022. Based on the inclusion and exclusion criteria, the literatures were strictly screened, and meanwhile the Revman 5.4 software was used to evaluate the migration risk of the included research included research and complete data analysis.
Results
7 RCT studies were included with a total of 396 adult patients.Among the stuey compared U-LRM with Non-LRM,RCTs showed a significant benefit of U-LRM for reducing the incidence of atelectasis at T3(RR = 0.52;95%CI [0.39, 0.69];P < 0.001;I² = 0%) and the LUS at T3(MD=-3.50; 95%CI[-5.06, -1.94];P < 0.001;I² = 86%).Among the stuey compared U-LRM with LRM, RCTs showed a significant benefit of U-LRM for reducing the incidence of atelectasis at T2(RR = 0.49;95%CI[0.36, 0.68];P < 0.001 I = 0%) and the LUS at T2(MD=-1.50; 95%CI[-2.90, -0.09];P = 0.04;I = 87%)and T3(MD=-1.58;95%CI [-2.68, -0.47];P = 0.005;I = 78%)
Conclusion
According to the study,ultrasound-guided lung recruitment maneuvers(U-LRM) may have an association with decreasing in the incidence of perioperative atelectasis and lung ultrasound scores. However,the result must be explained cautiously as the total patients is insufficient.
Spinal Echinococcus multilocularis infection mimicking tuberculosis: a rare case of vertebral alveolar echinococcosis in China
Authors: Li Chun , Luo Lei , Jin Da-di , Li Chuan-jiang , Yang Bin , Yang Ju-hua 
Published: April 2023

Abstract
Echinococcus multilocularis (E. multilocularis) infection can cause alveolar echinococcosis (AE), a zoonosis that rarely affects humans. Although the incidence of AE has increased substantially, its diagnosis remains challenging due to a lack of clinical manifestations and imaging findings specific to this disease. Here, we described a case of vertebral AE in an elderly male from south China, who was initially misdiagnosed as tuberculosis. After being diagnosed of E. multilocularis complicated with Serratia marcesceninfections by metagenomic next-generation sequencing, the patient received anti-infective treatment, and his condition improved and was finally discharged. Hence, clinicians should increase their awareness of AE as a cause for vertebral lesions, except malignancy and tuberculosis.
Exploring the role of healthcare personnel in designing Tuberculosis Infection Prevention and Control measures in healthcare settings: A scoping review
Authors: Masuku Sikhethiwe , Mooa Ramadimentja Shirley , Peu Mamphekho Dorrica , Ndwandwe Duduzile , Moloto Tebogo Brenda Sole , Walt Martie van der 
Published: April 2023

Abstract

Background
Healthcare personnel (HCP) in high TB burdened countries continue to be at high risk of occupational TB due to inadequate implementation of Tuberculosis Infection Prevention and Control (TB-IPC) measures, lack of understanding of the context and relevance to local settings. Such transmission in the healthcare workplace has prompted the development and dissemination of numerous guidelines for strengthening TB-IPC for use in settings globally. However, a growing body of literature points to lack of involvement of the HCP in the conceptualization and development of guidelines and programmes seeking to improve TB-IPC in high burden countries generally.
Objectives
The aim of this study is to identify factors affecting and influencing the adoption of TB-IPC measures in heath settings and the recognised research field exploring the inclusion of the HCP in decision making when designing these guidelines, in relation to appropriateness of the guidelines to the local context.
Methods
A scoping review methodology was selected for this study to gain insight into the relevant research evidence identifying and mapping key elements in the TB-IPC measures in relation to HCP as implementors.
Results
Studies in this review refer to factors related to HCP’s knowledge of TB-IPC, perception regarding occupational risks, behaviours, and their role against a background of structural resource constraints, and guidelines adherence. They report several challenges in the TB-IPC implementation and adherence particularly eliciting recommendations from HCP for improved TB-IPC practices.
Conclusion
Research on the enablers and barriers to TB-IPC implementation needs to go beyond mere documenting factors affecting and influencing adoption of TB-IPC measures in heath settings. There is an urgent need for research on participation of the implementers in the decision making when developing TB-IPC guidelines. Finally, when designing the TB-IPC guidelines, factors to be considered should be the appropriateness of the guidelines to the local context.
Construction and validation of prognostic risk model based on radiosensitivity-related immune genes in rectal cancer
Authors: Yang Hui , Liu Yin , Mu Xiaofeng , Wang Kun , Hao Mengdi , Li Huimin , Liang Xiaoqing , Yuan Dajin , Ding Lei 
Published: April 2023

Abstract
Background
Radiotherapy is closely related to the tumor immune microenvironment, but the role of immune genes in radiosensitivity and prognosis of rectal cancer (RC) is still unclear. This study aims to construct a prognostic risk model based on radiosensitivity-related immune genes (RRIGs), which can be used for predicting prognosis of RC.
Methods
GSE133057 dataset of RC was downloaded from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were identified between different radiosensitivity groups. RRIGs were obtained by intersecting DEGs and immune genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis were performed to study the biological functions of RRIGs. Transcriptomic and clinical data of RC were downloaded from The Cancer Genome Atlas (TCGA) database, and the entire cohort was randomly divided into training and testing set at a ratio of 7:3. Prognostic genes were selected by Cox analysis, and a risk model and nomogram were subsequently built. The relationship between the model and immune cell infiltration was analyzed by single-sample gene set enrichment analysis (ssGSEA).
Results
A total of 76 RRIGs were identified, and they were mainly involved in immune-related biological processes and pathways. BMP2, COLEC10, MASP2, and GCGR were screened as prognostic genes after Cox regression analysis. Subsequently, these prognostic genes were used to construct a risk score model, which demonstrated good performance in predicting prognosis, as proven by the receiver operating characteristic (ROC) curves. Cox regression analysis showed that the risk score was an independent prognostic factor for RC. Moreover, we found that the immune microenvironment was different between the low- and high-risk groups.
Conclusions
We developed and validated a prognostic risk model based on RRIGs, which could serve as a tool for predicting prognosis of RC. These findings enhanced the understanding of the relationship among radiosensitivity, immune genes and prognosis in RC.
Integrated muti-omics analyses and experimental validation reveal the clinical and biological Significances of APBB1IP in Colon Cancer
Authors: Ni Zhizhan , Chen Xin , Huang Qi , Zhou Jinzhe , Xie Huahao , Wang Song , Jiang Jiebang , Liu Liming , Ge Bujun 
Published: April 2023

Abstract
Background： Tumor-associated macrophages(TAMs), especially M2 macrophages, plays a critical role in Colorectal cancer initiation, promotion, and metastasis. However, the underlying mechanisms still remain unresolved.
Methods：The profile of ATAC-seq was employed to detect genes with open chromatin. The RNA-seq was used to identify differentially expressed genes (DEGs) and those DEGs with open chromatin in promoter regions were identified as hub gene. Then, CIBERSORT, quanTIseq and XCELL algorithm were employed to quantified the expression of M2 macrophages and Pearson correlation analysis was used to identified the relationship with M2 macrophages and hub gene. After that, the macrophages profile and scRNA-seq profile were used to identify the gene expression in different phenotype macrophage. GO/KEGG analysis, GSEA, GSVA were used for gene function analysis. Finally, the target gene which promote M2 macrophages polarization were further explored by experiment.
Results：In this study, we acquired 15,650 genes with open chromatin in promoter regions and 3,241 genes were identified as differential expression genes with open chromatin. Integrated CIBERSORT, quanTIseq and XCELL algorithm, we identified 72 genes were correlated with M2 macrophages (r>0.2, p<0.05). According to the macrophages profile from GEO database, 2 genes were identified as differential expressed genes which high expressed in M2 macrophages. By applying scRNA-seq, APBB1IP was the only gene expressed in macrophage and high expressed in M2 phenotype. M2 macrophage polarization were inhibited after knockdown of APBB1IP in vitro experiments.
Conclusion：APBB1IP, characterized by chromatin accessibility, downregulated in patients with COAD and induced M2 macrophage polarization.
The effectiveness of World Café-based flipped classroom to optimal interprofessional education among interprofessional healthcare workers: A quasi-experimental study
Authors: Li Hong-Ying , Hsu Tzu-Chuan , Huang Chu-Yu , Kuo Chien-Lin , Cheng Su-Fen 
Published: April 2023

Abstract
Background
Quality of healthcare outcomes rely heavily on effective interprofessional collaboration. However, inadequate communication and team collaboration may contribute to ineffective interprofessional collaborative practice (IPCP) and negatively impact patient safety. Traditionally, IPCP training sessions are conducted primarily through lectures with limited interactions across disciplines, which may create obstacles in cultivating effective interprofessional communication. Teaching strategies that combine the World Café approach with flipped classroom (WCFC) approach may improve team communication and collaboration. The purpose of this study was to evaluate the effectiveness of World Café-based flipped classroom (WCFC) approach in promoting interprofessional collaborative practice behaviors, learning engagement and learning satisfaction.
Methods
This quasi-experimental study used two-group repeated measure longitudinal design with a convenience sample. Seventy-six healthcare professionals participated in this study. The experimental group (n=38) completed a case studydiscussion course through the WCFC approach, whereas the control group (n=38) received the same course through a traditional teaching approach. Data collection took place before the intervention, one week and four weeks after the intervention. The instruments utilized were demographic information sheet, Lee’s (2016) Interprofessional Collaborative Practice Behavior Inventory (IPCPBI), Ciou’s (2020) Learning Engagement Scale, and Huang’s (2013) Learning Satisfaction Scale.
Results
There were no significant differences in the IPCPBI scores between the experimental and control groups one week after the intervention (p >.05). Analyses of the delay effects four weeks after the intervention foundsignificantly higher IPCPBI scores in the experimental group than the control group (p < .05). The learning engagement scores and learning satisfaction scores were higher in the experimental group but did not reach statistical significance (p> .05).
Conclusions
Case studydiscussions conducted using the WCFC approach effectively improved IPCPbehaviors, learning engagement and learning satisfaction in healthcare professionals. The World Café approach created a relaxing atmosphere for learning, and encouraged sharing among participants. Each participant experienced freedom and equal opportunities to contribute in interprofessional collaborative practice for the optimized care outcomes.