bluuebunny/crossref_metadata_2025_split huggingface dataset:
More Than Identity: An Intersectional Approach to Understanding Mental-Emotional Well-Being of Emerging Adults by Centering Lived Experiences of Marginalization
Authors: Slaughter-Acey Jaime , Simone Melissa , Hazzard Vivienne M , Arlinghaus Katherine R , Neumark-Sztainer Dianne 
Published: July 2023

Abstract
Understanding social determinants that shape pertinent developmental shifts during emerging adulthood (i.e., ages 18–25 years) and their associations with psychological health requires a nuanced approach. In our exploratory study, we investigated how multiple social identities and lived experiences generated by systems of marginalization and power (e.g., racism, classism, sexism) intersect in connection to the mental-emotional well-being of emerging adults (EAs). Eating and Activity Over Time (EAT, 2010–2018) data were collected from 1,568 EAs (mean age = 22.2 (standard deviation, 2.0) years) recruited initially in 2010 from Minneapolis/St. Paul schools. Conditional inference tree analyses were employed to treat “social location” and systems of marginalization and power as interdependent social factors influencing EAs’ mental-emotional well-being outcomes: depressive symptoms, stress, self-esteem, and self-compassion. Conditional inference tree analyses identified EAs’ subgroups with differing mean levels of mental-emotional well-being outcomes, distinguished primarily by marginalized social experiences (e.g., discrimination, financial difficulties) rather than social identities themselves. The relative positioning of EAs’ experiences of social marginalization (e.g., discrimination) to their social identities (e.g., race/ethnicity) suggests that the social experiences generated by systems of privilege and oppression (e.g., racism) are more adjacent social determinants of mental-emotional well-being than the social identities used in public health research to proxy the oppressive systems that give them social meaning.
Detailed Analysis of Three Major Breast Reconstructions Using BREAST-Q Responses From 1001 Patients
Authors: Tomita Shoichi , Nagai Keita , Matsunaga Noriko , Kerckhove Maiko de , Fujii Miwako , Terao Yasunobu 
Published: July 2023

Abstract

Background
Patient-reported outcomes have become as important as mortality and morbidity in the postoperative evaluation of breast reconstruction surgery. The BREAST-Q is one of the most widely used patient-reported outcomes for breast reconstruction.

Objectives
A comparative analysis of the scores on each of the BREAST-Q modules could help analyze different reconstruction methods. However, few studies have utilized BREAST-Q for this purpose. The aim of this study was therefore to compare breast reconstruction methods in terms of BREAST-Q module ratings.

Methods
The authors retrospectively reviewed the data of 1001 patients who had been followed for more than 1 year after breast reconstruction. The 6 BREAST-Q modules were rated on a scale of 0 to 100 and statistically analyzed by multiple regression. In addition, Fisher's exact test was performed after dividing the responses to each question into high- and low-rating groups.

Results
Microvascular abdominal flap reconstruction scored significantly better than implant-based reconstruction on all modules, except psychosocial and sexual well-being. In terms of satisfaction with the breast, latissimus dorsi flap reconstruction was superior to implant-based reconstruction. However, in terms of the reconstruction method, there were no differences in patients' willingness to make the same choice again or whether they regretted having surgery.

Conclusions
The results highlight the superiority of autologous breast reconstruction. Reconstruction methods should only be performed after a thorough explanation of their characteristics to achieve results that meet patient expectations. The findings are useful for facilitating patient decision-making in breast reconstruction.

Level of Evidence: 4
Beyond winglets: evolutionary scaling of flight-related morphology in stick insects (Phasmatodea)
Authors: Zeng Yu , Park Sehoon , Gonzales Camille , Yom Stephanie , Rahim Faszly , Dudley Robert 
Published: July 2023

Abstract
The first winged insects evolved from a wingless ancestor, but details of the transition to a fully-winged morphology remain unclear. Studying extant pterygotes with partial wings, such as the stick insects (order Phasmatodea), may help us to understand such a transition. Here, we address how a series of flight-related morphological parameters may correlate with flight evolution by studying different phasmids representing a volancy continuum ranging from miniaturized to full-sized wings. Variation in phasmid wing shape, venation, wing mass and the mass of flight muscle can be described by specific scaling laws referenced to wing length and wing loading. Also, the mass distribution of the body-leg system is conserved in spite of a wide range of variation in body shape. With reduced wing size and increased wing loading, the longitudinal position of the wing-bearing thoracic segments is shifted closer to the insects’ centre of body mass. These results demonstrate complex reconfiguration of the flight system during wing morphological transitions in phasmids, with various anatomical features potentially correlated with reduced flight performance attained with partial wings. Although these data represent phasmid-specific features of the flight apparatus and body plan, the associated scaling relationships can provide insight into the functionality of intermediate conditions between wingless and fully-winged insects more generally.
Repeated evolution of pollination syndromes in a highly diverse bromeliad lineage is correlated with shifts in life form and habitat
Authors: Neves Beatriz , Ferreira Paola de L , Prosdocimi Francisco , Kessous Igor M , Couto Dayvid R , Moura Ricardo L , Salgueiro Fabiano , Costa Andrea F , Bacon Christine D , Antonelli Alexandre 
Published: July 2023

AbstractMutualistic plant-pollinator interactions play a critical role in the diversification of flowering plants. The spatiotemporal correlates of such interactions can be understood in a phylogenetic context. Here, we generate ddRAD-seq data for the highly diverse Vriesea-Stigmatodon lineage to test for correlated trait evolution among pollination syndromes and life form, habitat type, and altitude. Our results show that pollination syndromes are correlated with changes in life form and habitat type. The ancestor of the Vriesea-Stigmatodon lineage was likely bat pollinated, rock dwelling and inhabited open, mid-elevation forests. Transitions from bat to hummingbird pollination are correlated with transitions to the epiphytic life form in shaded habitats, whereas bat pollination is correlated with the rock-dwelling life form and open habitats. Our dated phylogenetic tree reveals independent origins of hummingbird pollination, occurring twice in Vriesea at c. 5.8 and 5.4 Mya. The timing for the shifts in pollination syndrome coincides with geological and environmental transformations across the Serra do Mar Mountain Chain, which increased habitat heterogeneity where Vriesea and their mutualists diversified. The phylogenetic tree reinforces the non-monophyly of taxonomic sections within the genus Vriesea previously defined by flower morphology, indicating that some lineages should be treated as species complexes. This study identifies synergetic drivers of speciation in a tropical biodiversity hotspot.
RNF213 loss-of-function promotes pathological angiogenesis in moyamoya disease via the Hippo pathway
Authors: Ye Fei , Niu Xingyang , Liang Feng , Dai Yuanyuan , Liang Jie , Li Jiaoxing , Wu Xiaoxin , Zheng Hanyue , Qi Tiewei , Sheng Wenli 
Published: July 2023

Abstract
Moyamoya disease is an uncommon cerebrovascular disorder characterized by steno-occlusive changes in the circle of Willis and abnormal vascular network development. Ring finger protein 213 (RNF213) has been identified as an important susceptibility gene for Asian patients, but researchers have not completely elucidated whether RNF213 mutations affect the pathogenesis of moyamoya disease.
Using donor superficial temporal artery samples, whole-genome sequencing was performed to identify RNF213 mutation types in patients with moyamoya disease, and histopathology was performed to compare morphological differences between patients with moyamoya disease and intracranial aneurysm. The vascular phenotype of RNF213-deficient mice and zebrafish was explored in vivo, and RNF213 knockdown in human brain microvascular endothelial cells was employed to analyse cell proliferation, migration and tube formation abilities in vitro. After bioinformatics analysis of both cell and bulk RNA-seq data, potential signalling pathways were measured in RNF213-knockdown or RNF213-knockout endothelial cells.
We found that patients with moyamoya disease carried pathogenic mutations of RNF213 that were positively associated with moyamoya disease histopathology. RNF213 deletion exacerbated pathological angiogenesis in the cortex and retina. Reduced RNF213 expression led to increased endothelial cell proliferation, migration and tube formation. Endothelial knockdown of RNF213 activated the Hippo pathway effector Yes-associated protein (YAP)/tafazzin (TAZ) and promoted the overexpression of the downstream effector VEGFR2. Additionally, inhibition of YAP/TAZ resulted in altered cellular VEGFR2 distribution due to defects in trafficking from the Golgi apparatus to the plasma membrane and reversed RNF213 knockdown-induced angiogenesis. All these key molecules were validated in ECs isolated from RNF213-deficient animals.
Our findings may suggest that loss-of-function of RNF213 mediates the pathogenesis of moyamoya disease via the Hippo pathway.
Efficacy of anti-programmed cell death protein 1 monoclonal antibody combined with bevacizumab and/or Pseudomonas aeruginosa injection in transplanted tumor of mouse forestomach carcinoma cell gastric cancer in mice and its mechanism in regulating tumor immune microenvironment
Authors: Liu Xiangyong , Yan Chao , Yang Aijie , Yu Enhao , Yu Jie , Zhou Chunyang , Wang Yun , Wang Kai , Sun Ying , Cheng Yufeng 
Published: July 2023

Abstract
Tumor immunotherapy represented by programmed cell death protein 1 (PD-1) inhibitors is considered as the most promising cancer treatment method and has been widely used in the treatment of advanced gastric cancer (GC). However, the effective rate of PD-1 inhibitor monotherapy is low. In this study, we constructed a transplanted tumor model in GC mice by inoculating mouse forestomach carcinoma cell (MFC) GC cells into 615 mice. Interventions were conducted with normal saline, anti-PD-1 monoclonal antibody (mAb), bevacizumab, Pseudomonas aeruginosa-mannose-sensitive hemagglutinin (PA-MSHA), anti-PD-1 mAb combined with bevacizumab, anti-PD-1 mAb combined with PA-MSHA, bevacizumab combined with PA-MSHA, anti-PD-1 mAb combined with bevacizumab and PA-MSHA, respectively. The tumor growth curves were drawn. TUNEL assay, western blotting, and immunohistochemistry were used to detect tumor proliferation and apoptosis. Flow cytometry and ELISA were used to detect the expression of tumor infiltrating lymphocytes and cytokines. This study found that anti-PD-1 mAb alone could not significantly inhibit the growth of transplanted tumors in mice. Anti-PD-1 mAb combined with bevacizumab, anti-PD-1 mAb combined with PA-MSHA, anti-PD-1 mAb combined with bevacizumab and PA-MSHA could all significantly inhibit tumor growth in mice, and the combination of three drugs presented the highest tumor inhibition rate. Anti-PD-1 mAb combined with bevacizumab and PA-MSHA could significantly upregulate the number of Th1-type cells, CD8 + T cells, and Type I tumor-associated macrophages (TAMs), while downregulate the number of Th2-type cells, myeloid-derived suppressor cells, regulatory T cells, and Type II TAMs. Therefore, we conclude that anti-PD-1 mAb combined with bevacizumab and/or PA-MSHA has a synergistic effect. Bevacizumab and PA-MSHA can transform the tumor immunosuppressive microenvironment into a supportive immune microenvironment, thus maximizing the antitumor effect of anti-PD-1 mAb.
ChatGPT, GPT-4, and Other Large Language Models: The Next Revolution for Clinical Microbiology?
Authors: Egli Adrian 
Published: July 2023

Abstract
ChatGPT, GPT-4, and Bard are highly advanced natural language process–based computer programs (chatbots) that simulate and process human conversation in written or spoken form. Recently released by the company OpenAI, ChatGPT was trained on billions of unknown text elements (tokens) and rapidly gained wide attention for its ability to respond to questions in an articulate manner across a wide range of knowledge domains. These potentially disruptive large language model (LLM) technologies have a broad range of conceivable applications in medicine and medical microbiology. In this opinion article, I describe how chatbot technologies work and discuss the strengths and weaknesses of ChatGPT, GPT-4, and other LLMs for applications in the routine diagnostic laboratory, focusing on various use cases for the pre- to post-analytical process.
Premature deindustrialisation: the international evidence
Authors: Özçelik Emre , Özmen Erdal 
Published: July 2023

Abstract
We examine patterns and globalisation-related causes of premature deindustrialisation (PD) in recent decades, using a large panel of advanced, emerging and developing economies (AE, EME and DE). The results verify the existence of PD in EME and DE, except East Asian countries. African countries have been worst affected by PD. Globalisation-related determinants of PD vary across country groups. While trade openness leads to deindustrialisation in DE; it enhances ‘dependent’ industrialisation in Latin American countries and the ‘factory economies’ of East Asia, which have stronger linkages to global value chains. Financial openness fosters industrialisation in severely finance-constrained economies, whereas it brings about deindustrialisation in financially stronger ones. It is our contention that development possibilities can be expanded by aiming at more intense linkages to global value chains, but proactive industrial policies at the levels of EME and DE are required to achieve such expansion.
Disease Recurrence and Long-term Outcomes Following the Development of Intestinal Failure in Crohn’s Disease: Over 20 Years of Experience from a National Reference Centre
Authors: Kopczynska Maja , Crooks Benjamin , Deutsch Liat , Conley Thomas , Stansfield Catherine , Bond Ashley , Soop Mattias , Carlson Gordon , Lal Simon 
Published: July 2023

Abstract

Background and Aims
Intestinal failure [IF] is a recognised complication of Crohn’s disease [CD]. The aim of this study was to identify factors predicting the development and recurrence of CD in patients with IF [CD-IF], and their long-term outcomes.

Methods
This was a cohort study of adults with CD-IF admitted to a national UK IF reference centre between 2000 and 2021. Patients were followed from discharge with home parenteral nutrition [HPN] until death or February 28, 2021.

Results
In all, 124 patients were included; 47 [37.9%] changed disease location and 55 [44.4%] changed disease behaviour between CD and CD-IF diagnosis, with increased upper gastrointestinal involvement [4.0% vs 22.6% patients], p &lt;0.001. Following IF diagnosis, 29/124 [23.4%] patients commenced CD prophylactic medical therapy; 18 [62.1%] had a history of stricturing or penetrating small bowel disease; and nine [31.0%] had ileocolonic phenotype brought back into continuity. The cumulative incidence of disease recurrence was 2.4% at 1 year, 16.3% at 5 years and 27.2% at 10 years; colon-in-continuity and prophylactic treatment were associated with an increased likelihood of disease recurrence. Catheter-related bloodstream infection [CRBSI] rate was 0.32 episodes/1000 catheter days, with no association between medical therapy and CRBSI rate.

Conclusions
This is the largest series reporting disease behaviour and long-term outcomes in CD-IF and the first describing prophylactic therapy use. The incidence of disease recurrence was low. Immunosuppressive therapy appears to be safe in HPN-dependent patients with no increased risk of CRBSI. The management of CD-IF needs to be tailored to the patient’s surgical disease history alongside disease phenotype.
Multi-modality imaging in aortic stenosis: an EACVI clinical consensus document
Authors: Dweck Marc R , Loganath Krithika , Bing Rong , Treibel Thomas A , McCann Gerry P , Newby David E , Leipsic Jonathon , Fraccaro Chiara , Paolisso Pasquale , Cosyns Bernard , Habib Gilbert , Cavalcante João , Donal Erwan , Lancellotti Patrizio , Clavel Marie-Annick , Otto Catherine M , Pibarot Phillipe 
Published: July 2023

Abstract
In this EACVI clinical scientific update, we will explore the current use of multi-modality imaging in the diagnosis, risk stratification, and follow-up of patients with aortic stenosis, with a particular focus on recent developments and future directions. Echocardiography is and will likely remain the key method of diagnosis and surveillance of aortic stenosis providing detailed assessments of valve haemodynamics and the cardiac remodelling response. Computed tomography (CT) is already widely used in the planning of transcutaneous aortic valve implantation. We anticipate its increased use as an anatomical adjudicator to clarify disease severity in patients with discordant echocardiographic measurements. CT calcium scoring is currently used for this purpose; however, contrast CT techniques are emerging that allow identification of both calcific and fibrotic valve thickening. Additionally, improved assessments of myocardial decompensation with echocardiography, cardiac magnetic resonance, and CT will become more commonplace in our routine assessment of aortic stenosis. Underpinning all of this will be widespread application of artificial intelligence. In combination, we believe this new era of multi-modality imaging in aortic stenosis will improve the diagnosis, follow-up, and timing of intervention in aortic stenosis as well as potentially accelerate the development of the novel pharmacological treatments required for this disease.
Long-term outcome of patients with combined post- and pre-capillary pulmonary hypertension
Authors: Titz Anna , Mayer Laura , Appenzeller Paula , Müller Julian , Schneider Simon R , Tamm Michael , Darie Andrei M , Guler Sabina A , Aubert John-David , Lador Frédéric , Stricker Hans , Fellrath Jean-Marc , Pohle Susanne , Lichtblau Mona , Ulrich Silvia 
Published: July 2023

Abstract

Aims
Pulmonary hypertension (PH) is a complex clinical condition, and left heart disease is the leading cause. Little is known about the epidemiology and prognosis of combined post- and pre-capillary PH (CpcPH).

Methods and results
This retrospective analysis of the Swiss PH Registry included incident patients with CpcPH registered from January 2001 to June 2019 at 13 Swiss hospitals. Patient baseline characteristics [age, sex, mean pulmonary artery pressure (mPAP), pulmonary artery wedge pressure (PAWP), pulmonary vascular resistance (PVR), and risk factors, including World Health Organization (WHO)-functional class (FC), 6 min walk distance (6MWD), and N-terminal pro-brain natriuretic peptide (NT-proBNP), treatment, days of follow-up, and events (death or loss to follow-up) at last visit] were analysed by Kaplan–Meier and Cox regression analyses. Two hundred and thirty-one patients (59.3% women, age 65 ± 12 years, mPAP 48 ± 11 mmHg, PAWP 21 ± 5 mmHg, PVR 7.2 ± 4.8 WU) were included. Survival analyses showed a significantly longer survival for women [hazard ratio (HR) 0.58 (0.38–0.89); P = 0.01] and a higher mortality risk for mPAP &gt; 46 mmHg [HR 1.58 (1.03–2.43); P = 0.04] but no association with age or PVR. Patients stratified to high risk according to four-strata risk assessment had an increased mortality risk compared with patients stratified to low-intermediate risk [HR 2.44 (1.23–4.84); P = 0.01]. A total of 46.8% of CpcPH patients received PH-targeted pharmacotherapy; however, PH-targeted medication was not associated with longer survival.

Conclusion
Among patients with CpcPH, women and patients with an mPAP ≤46 mmHg survived longer. Furthermore, risk stratification by using non-invasively assessed risk factors, such as WHO-FC, 6MWD, and NT-proBNP, as proposed for pulmonary arterial hypertension, stratified survival in CpcPH, and might be helpful in the management of these patients.
Identification of grapevine clones via high-throughput amplicon sequencing: a proof-of-concept study
Authors: Urra Claudio , Sanhueza Dayan , Pavez Catalina , Tapia Patricio , Núñez-Lillo Gerardo , Minio Andrea , Miossec Matthieu , Blanco-Herrera Francisca , Gainza Felipe , Castro Alvaro , Cantu Dario , Meneses Claudio 
Published: July 2023

Abstract
Wine cultivars are available to growers in multiple clonal selections with agronomic and enological differences. Phenotypic differences between clones originated from somatic mutations that accrued over thousands of asexual propagation cycles. Genetic diversity between grape cultivars remains unexplored, and tools to discriminate unequivocally clones have been lacking. This study aimed to uncover genetic variations among a group of clonal selections of 4 important Vitis vinifera cultivars: Cabernet sauvignon, Sauvignon blanc, Chardonnay, and Merlot, and use this information to develop genetic markers to discriminate the clones of these cultivars. We sequenced with short-read sequencing technology the genomes of 18 clones, including biological replicates for a total of 46 genomes. Sequences were aligned to their respective cultivar's reference genome for variant calling. We used reference genomes of Cabernet sauvignon, Chardonnay, and Merlot and developed a de novo genome assembly of Sauvignon blanc using long-read sequencing. On average, 4 million variants were detected for each clone, with 74.2% being single nucleotide variants and 25.8% being small insertions or deletions (InDel). The frequency of these variants was consistent across all clones. From these variants, we validated 46 clonal markers using high-throughput amplicon sequencing for 77.7% of the evaluated clones, most of them small InDel. These results represent an advance in grapevine genotyping strategies and will benefit the viticulture industry for the characterization and identification of the plant material.
Social Trust, Human Values, and Anti-immigrant Sentiment: Does Trust Work With or Against Values?
Authors: Ponce Aaron 
Published: July 2023

Abstract
Two separate lines of literature have analyzed the role social trust and human values, such as universalism and conservatism, have on anti-immigrant sentiment. While both show strong effects, the question of whether social trust weakens or strengthens already embodied values has not been addressed. The possibility that trust works against or with conservatism, for example, predicts two opposing forces on anti-immigrant sentiment. This study analyzes the potential moderating effect of trust on human values, and how this varies cross-nationally. It develops a conceptual framework that hypothesizes different models of trust and tests these using eight rounds of the European Social Survey (2002–2016). Findings point to trust consistently working with values, which suggests a magnification of conservative values’ anti-immigrant effects. While there is little evidence for national cultures of trust and values, country-specific results show variation in how trust moderates values, with greater evidence for trust’s moderation of conservatism and some unpredicted patterns in countries like Cyprus. Findings are discussed within the context of anti-immigrant politics and entrenched values, scholarly debates surrounding bridging and bonding, and the resulting extent of social trust’s radius.