bluuebunny/crossref_metadata_2025_split huggingface dataset:
SF3B1-dependent alternative splicing is critical for maintaining endometrial homeostasis and the establishment of pregnancy
Authors: Popli Pooja , Chadchan Sangappa B. , Dias Michelle , Deng Xinchao , Gunderson Stephanie J. , Jimenez Patricia , Yalamanchili Hari , Kommagani Ramakrishna 
Published: May 2023

AbstractThe remarkable potential of human endometrium to undergo spontaneous remodeling is shaped by controlled spatiotemporal gene expression patterns. Although hormone-driven transcription shown to govern these patterns, the post-transcriptional processing of these mRNA transcripts, including the mRNA splicing in the endometrium is not studied yet. Here, we report that the splicing factor, SF3B1 is central in driving alternative splicing (AS) events that are vital for physiological responses of the endometrium. We show that loss of SF3B1 splicing activity impairs stromal cell decidualization as well as embryo implantation. Transcriptomic analysis revealed that SF3B1 depletion decidualizing stromal cells led to differential mRNA splicing. Specifically, a significant upregulation in mutually exclusive AS events (MXEs) with SF3B1 loss resulted in the generation of aberrant transcripts. Further, we found that some of these candidate genes phenocopy SF3B1 function in decidualization. Importantly, we identify progesterone as a potential upstream regulator of SF3B1-mediated functions in endometrium possibly via maintaining its persistently high levels, in coordination with deubiquitinating enzymes. Collectively, our data suggest that SF3B1-driven alternative splicing plays a critical role in mediating the endometrial-specific transcriptional paradigms. Thus, the identification of novel mRNA variants associated with successful pregnancy establishment may help to develop new strategies to diagnose or prevent early pregnancy loss.
Neuronal Branching is Increasingly Asymmetric Near Synapses, Potentially Enabling Plasticity While Minimizing Energy Dissipation and Conduction Time
Authors: Desai-Chowdhry Paheli , Brummer Alexander B. , Mallavarapu Samhita , Savage Van M 
Published: May 2023

AbstractNeurons’ primary function is to encode and transmit information in the brain and body. The branching architecture of axons and dendrites must compute, respond, and make decisions while obeying the rules of the substrate in which they are enmeshed. Thus, it is important to delineate and understand the principles that govern these branching patterns. Here, we present evidence that asymmetric branching is a key factor in understanding the functional properties of neurons. First, we derive novel predictions for asymmetric scaling exponents that encapsulate branching architecture associated with crucial principles such as conduction time, power minimization, and material costs. We compare our predictions with extensive data extracted from images to associate specific principles with specific biophysical functions and cell types. Notably, we find that asymmetric branching models lead to predictions and empirical findings that correspond to different weightings of the importance of maximum, minimum, or total path lengths from the soma to the synapses. These different path lengths quantitatively and qualitatively affect energy, time, and materials. Moreover, we generally observe that higher degrees of asymmetric branching— potentially arising from extrinsic environmental cues and synaptic plasticity in response to activity— occur closer to the tips than the soma (cell body).
Focusing attention in human working memory and long-term memory: benefits through dissociable processes
Authors: Gong Dongyu , Draschkow Dejan , Nobre Anna C. 
Published: May 2023

AbstractWe developed a new experimental approach to compare how attentional orienting facilitates retrieval from working memory (WM) and long-term memory (LTM), and how selective attention within these two memory types impacts incoming sensory information processing. In three experiments with healthy young adults, retrospective attention cues prioritized an item represented in WM or LTM. Participants then retrieved a memory item or performed a perceptual task. The retrocue was informative for the retrieval task but not for the perceptual task. Attentional orienting benefited performance for both WM and LTM, with stronger effects for WM. Eye-tracking revealed significant gaze shifts and microsaccades correlated with attention in WM but not LTM. Visual discrimination of unrelated visual stimuli was consistently improved for items matching attended WM locations. Similar effects occurred at LTM locations but less consistently. The findings suggest potent and at least partly dissociable attention-orienting processes for different memory types.
Hijacking of internal calcium dynamics by intracellularly residing rhodopsins
Authors: Eria-Oliveira Ana-Sofia , Folacci Mathilde , Chassot Anne Amandine , Fedou Sandrine , Thézé Nadine , Zabelskii Dmitrii , Alekseev Alexey , Bamberg Ernst , Gordeliy Valentin , Sandoz Guillaume , Vivaudou Michel 
Published: May 2023

AbstractRhodopsins are ubiquitous light-driven membrane proteins with diverse functions, including ion transport. Widely distributed, they are also coded in the genomes of giant viruses infecting phytoplankton where their function is not settled. We examined the properties of three type 1 viral channelrhodopsins (VCR1s), and, unexpectedly, found that VCR1s accumulate exclusively intracellularly, and, upon illumination, induced calcium release from intracellular IP3-dependent stores. In vivo, this light-induced calcium release was sufficient to remote control muscle contraction and behavior in VCR1-expressing tadpoles. VCR1s are the first rhodopsins shown to natively confer light-induced Ca2+release, suggesting an original mechanism for reshaping the response to light of virus-infected algae. The ability of VCR1s to photorelease calcium without altering plasma membrane electrical properties marks them as precursors for novel optogenetics tools, with potential applications in basic research and medicine.
Eighteen‐year nitrogen addition does not increase plant phosphorus demand in a nitrogen‐saturated tropical forest
Authors: Yu Guangcan , Chen Jing , Yu Mengxiao , Li Andi , Wang Ying‐Ping , He Xinhua , Tang Xuli , Liu Hui , Jiang Jun , Mo Jiangming , Zhang Shuo , Yan Junhua , Zheng Mianhai 
Published: May 2023

Abstract

Nitrogen (N) deposition usually increases plant tissue N concentrations and thus phosphorus (P) demand in young and/or N‐limited forests, but the N deposition effect on plant P demand has rarely been assessed in N‐saturated forests.

Impacts of 18‐year external N additions (Control: 0, Low N: 50, Moderate N:100 and High N: 150 kg N ha−1 year−1) on leaf P of four plant life‐forms (tree, shrub, herb and liana), P fractions of bulk and rhizosphere soils were examined in a N‐saturated mature tropical forest in southern China.

Leaf N, P and N: P ratios of all plant life‐forms remained stable under three N additions. Among soil P fractions, moderate labile organic P increased by 25%–33% across three N additions; and soil total P was increased by 11.76% under Low N, and 8.87% under High N, compared with the control. The PLS‐PM results showed that path coefficient of microbial community to available P significantly increased and of inorganic P to available P significantly decreased under N additions than control. N additions improved soil P availability through microbe‐mediated P transformation: Low N significantly increased soil microbial taxonomic diversity, and a higher microbial diversity could enlarge the sources of nutrient acquisition and stimulate decomposition of recalcitrant organic matters; while High N significantly decreased soil microbial taxonomic diversity, the remaining microorganisms that were screened by N‐rich environments had the characteristics of resisting the N addition effects and maintained efficient P acquisition.

Synthesis. Our findings provide a novel line of evidence that long‐term N deposition did not increase plant P demand in a N‐saturated mature tropical forest. The underlying mechanism is that plants did not increase N uptakes therefore nor increase P uptakes (a stable leaf N: P stoichiometry) in an already N‐saturated ecosystem. Different N addition rates regulated soil P transformation via microbial community transition. These findings help improve the understanding of plant P acquisition and modelling of biogeochemical N–P cycling and vegetation productivity in N‐rich forest ecosystems, particularly considering the fact that chronic N deposition may likely lead to soil N richness and even saturation of many forests in the future.
Enoxaparin initiation after chronic subdural haematoma evacuation: a randomized clinical trial on timing and outcomes
Authors: Shafiei Mehdi , Sabouri Masih , Aminmansour Bahram , Mahmoodkhani Mehdi , Sourani Arman , Salehi Iman , Foroughi Mina 
Published: May 2023

AbstractAimPatients with chronic subdural haematoma (CSDH) are at a significant risk for venous thromboembolism (VTE). Surgeons should weigh the advantages versus disadvantages of anticoagulants in the postoperative period.Patients and MethodsThis study was a randomized clinical trial conducted in Isfahan, Iran, from May 2016 to April 2021. Patients with CSDH eligible for bur‐hole craniostomy were primarily enrolled. All of them underwent bilateral lower limb Doppler ultrasonography (DUS) for deep venous thrombosis (DVT) screening. The patients were randomized into the case (n = 66, enoxaparin initiation 24 h after operation) and control (n = 70, enoxaparin initiation 72 h after operation) groups. Routine postoperative brain computed tomography scans were obtained 1 and 3 days after surgery. A second DUS was performed 96 h after operation to screen newly developed venous thrombosis; P value <.05 was defined significant.ResultsA total of 73 patients (59.8%) were female and 49 (40.2%) were male. The mean age was 65.1 ± 10.19 years; 9.9% of the patients had previously used antiplatelets. One patient had asymptomatic preoperative DVT. The mean values for enoxaparin dosage were 40.4918 ± 5.43 mg/day. Postoperative DVT or rebleeding prevalence was 0% in both groups. The mean follow‐up duration was 19.139 ± 2.2 months. Long‐term recurrence rate was 2.4% (n = 3). Postoperative pneumocephalus was associated with a higher recurrence rate (P = .031).ConclusionIn terms of VTE chemoprophylaxis, following bur‐hole craniostomy for CSDH, enoxaparin will effectively prevent VTE development without any clinically significant rebleeding.
Segment 3 cholangiojejunostomy for unresectable peri‐hilar cholangiocarcinoma: A case report and literature review
Authors: Tidjane Anisse , Boudjenan Nabil , Ikhlef Nacim , Bengueddach Aicha , Meharzi Sif‐Elislem , Larbi Hakim , Ourabah Anissa , Tabeti Benali 
Published: May 2023

AbstractSurgical management of unresectable perihilar cholangiocarcinoma is no longer relevant. Often these patients benefit from less invasive endoscopic or radiological management, but in the event of failure of these techniques, and in selected patients, cholangiojejunostomy in segment 3 can be an effective option. We describe the case of a 48‐year‐old patient presenting with an unresectable perihilar cholangiocarcinoma, in whom palliative endoscopic management failed two times and an open segment 3 intrahepatic cholangiojejunostomy was performed. The patient was discharged on the 7th day, has a complete resolution of jaundice at 2 months and is alive at 6 months without any readmission.
AMPK activation alleviated dextran sulfate sodium‐induced colitis by inhibiting ferroptosis
Authors: Sun Shao Peng , Lu Yi Fan , Li Heng , Weng Chun Yan , Chen Jia Jia , Lou Yi Jie , Lyu Dong , Lyu Bin 
Published: May 2023

ObjectivesFerroptosis is a newly discovered cell death mode that has been confirmed to occur in the intestinal epithelial cells in ulcerative colitis (UC). In this study we aimed to elucidate the mechanism of ferroptosis and its association with adenosine monophosphate‐activated protein kinase (AMPK) in UC.MethodsGene expression profiles of colonic mucosa (GSE87473) were downloaded. Both human colonic samples and dextran sodium sulfate (DSS)‐induced colitis murine model were used. The molecular markers of ferroptosis were detected using western blot and immunohistochemistry. Symptoms, iron abundance, and lipid peroxidation level of the mouse model were measured to evaluate the role of AMPK activation in ferroptosis.ResultsBoth gene and protein expressions of GPX4 and FTH1 were decreased in UC patients compared with the healthy controls. An increased iron abundance and lipid peroxidation level in colon tissues and damaged mitochondria were found in DSS‐induced colitis. AMPK expression was decreased in UC patients and correlated with FTH1 and GPX4. Activation of AMPK with metformin inhibited ferroptosis in the colon, improved symptoms, and prolonged the lifespan in DSS‐induced colitis mice.ConclusionsFerroptosis can be observed in colonic tissues in UC. AMPK activation inhibits ferroptosis in murine colitis model, which may act as a potential target for the treatment of colitis.
Efficacy of new hemostatic techniques in nonvariceal gastrointestinal bleeding: A systematic review and network meta‐analysis
Authors: Liu Kai , Gao Li , Bai Jia Wei , Wang Lu Lu , Zhu Shao Hua , Zhao Xin , Han Ying , Liu Zhi Guo 
Published: May 2023

ObjectivesNonvariceal gastrointestinal bleeding (NVGIB) is a common medical condition with significant mortality and morbidity. Several types of hemostatic modalities are currently available in clinical setting. This systematic review and network meta‐analysis aimed to assess the efficacy of these modalities in treating NVGIB.MethodsPubMed, EMBASE, and the Cochrane Library databases were searched for studies that compared the efficacy of different hemostatic techniques (over‐the‐scope clip [OTSC], hemostatic powder [HP] and conventional endoscopic treatment [CET]) for NVGIB published up to June 2022. The 30‐day rebleeding rate was regarded as the primary outcome. We performed pairwise and network meta‐analyses for all treatments. The heterogeneity and transitivity were evaluated.ResultsTwenty‐two studies were included. Regarding the 30‐day rebleeding rate, OTSC and HP plus CET showed superior efficacy in treating NVGIB compared with CET (OTSC vs CET: relative risk [RR] 0.42, 95% confidence interval [CI] 0.28–0.60; HP plus CET vs CET: RR 0.40, 95% CI 0.17–0.87), while OTSC and HP plus CET had comparable efficacy (RR 0.95, 95% CI 0.38–2.31). HP plus CET ranked the highest in the network ranking estimate. The sensitivity analysis showed that it was not robust that OTSC was superior to CET regarding the short‐term rebleeding rate and the initial hemostasis rate. While all‐cause mortality, bleeding‐related mortality and necessity of surgical or angiographic salvage therapy showed no statistically significant difference.ConclusionOTSC and HP plus CET significantly reduced 30‐day rebleeding rate compared to CET and had comparable efficacy in the treatment of NVGIB.
A comprehensive analysis of partial peristalsis recovery after peroral endoscopic myotomy in patients with achalasia
Authors: Shi Liang Liang , Yang Tian , Jiang Ni , Lyu Ying , Guo Hui Min , Yang Hua , Yin Qin , Zou Xiao Ping , Zhang Ni Na 
Published: May 2023

ObjectiveTo determine whether peroral endoscopic myotomy (POEM) improves esophageal peristalsis and to investigate the association between recovery of esophageal peristalsis after POEM and clinical features of the patients.MethodsIn this single‐center retrospective study, data were collected from medical records of the patients with achalasia who underwent POEM between January 2014 and May 2016. Demographics data, high‐resolution esophageal manometry parameters, Eckardt score, and gastroesophageal reflux disease questionnaire (GERD‐Q) score were collected. Weak and fragmented contraction was defined as partial recovery of esophageal peristalsis based on the Chicago classification version 3.0. Logistic regression analysis was used to identify variables associated with the partial recovery of peristalsis after POEM.ResultsA total of 103 patients were enrolled. Esophageal contractile activity was observed in the distal two‐thirds of the esophagus in 24 patients. The Eckardt score, integrated relaxation pressure, and lower esophageal sphincter (LES) resting pressure were significantly decreased after POEM. Multivariate analysis revealed that preprocedural LES resting pressure (P = 0.013) and preprocedural Eckardt score (P = 0.002) were related to the partial recovery of peristalsis after POEM. Symptoms of gastroesophageal reflux and reflux esophagitis after POEM were less frequent in those with partial recovery of peristalsis (both P < 0.05).ConclusionsNormalization of esophagogastric junction relaxation pressure achieved by POEM is associated with the partial recovery of esophageal peristalsis in patients with achalasia. Preprocedural LES resting pressure and the Eckardt score are predictive of the recovery of esophageal peristalsis.
Peer effects in subjective performance evaluation
Authors: Cassar Gavin , Ko Taeho 
Published: May 2023

AbstractWe investigate the influence of peer quality on subjective performance evaluation using 75,413 ratings of 130 employees from 6,908 raters in a business school setting. We find that subjective performance ratings are lower for employees with higher quality peer groups in both randomized and nonrandomized settings. Using a novel long‐window setting, we observe peer effects persisting, but slowly decaying, for several months even when priming raters with the employees' previous performance information. We find that the strength of the peer effects is greater for focal employees with weaker performance, for the peers with higher attribute similarity, and when the performance of peers is more extreme. Overall, we find strong and persistent peer effects in subjective performance evaluation.
Corticolous lichen communities in Algerian climax forests
Authors: Boutabia Lamia , Menaa Mohcen , Telailia Salah 
Published: May 2023

AbstractThe great extension of forests in the Djebel El Ghorra massif of El Kala National Park (north‐eastern Algeria) allowed us to recognise three distinct forest formations: cork oak stands (Quercus suber), zeen oak stands (Quercus canariensis) and mixed oak (Quercus suber and Quercus canariensis) forest. Their peculiarities showed ideal ecological characteristics for the colonisation of corticolous lichens the two phorophytes. The three forest formations were sampled between 2017 and 2022. The identification of the taxa is based on stereo‐microscopic examinations and usual chemical tests. Systematic surveys were conductedand allowed us to establish a list of 141 lichen taxa divided into 30 families.
Microdissection testicular sperm extraction outcomes in azoospermic patients with bilateral orchidopexy
Authors: Arasteh Hamid , Gilani Mohammad Ali Sadighi , Ramezani‐binabaj Mahdi , Babaei Mehdi 
Published: May 2023

AbstractBackgroundCryptorchidism is considered to be one of the most common causes of non‐obstructive azoospermia. There are several surgical techniques to retrieve sperm in these patients. Microdissection testicular sperm extraction (m‐TESE) is a recent sperm retrieval technique which is considered to be a safe, non‐blind, and feasible method.ObjectivesThis study aimed to investigate sperm retrieval rate (SRR) by the mTESE method in patients who have undergone orchidopexy due to bilateral cryptorchidism.Materials and methodsIn this retrospective study, 56 ex‐cryptorchid patients, who underwent mTESE due to post orchidopexy azoospermia, were included. Patients with hypogonadotropic hypogonadism, Klinefelter syndrome, azoospermia factors (AZF) microdeletion, or chromosomal translocation were excluded from the study. Data were obtained from medical files.ResultsSRR in this study was 46%. Patients were divided into two groups of negative (n = 30) and positive (n = 26) based on the sperm extraction outcomes. There was no statistically significant difference between two groups regarding the mean age at mTESE, mean age at orchidopexy, testicular size, and serum testosterone concentration. However, testicular location, histological patterns, FSH, and LH level showed to have statistically significant relation with sperm retrieval results. But, according to our logistic regression, none of the included variable in the model including FSH, LH, histopathology, and testis location have a significant effect on the presence of the sperm.DiscussionIn the present study, SRR was significantly higher in patients with scrotal testis and low level of FSH and LH.ConclusionsPerforming mTESE could be recommended in ex‐cryptorchid patients with post orchidopexy NOA. Preoperative testicular biopsy seems to be unnecessary while clinical criteria can perfectly define NOA.
How is companionship related to romantic partners' affect, relationship satisfaction, and health behavior? Using a longitudinal dyadic score model to understand daily and couple‐level effects of a dyadic predictor
Authors: Stadler Gertraud , Scholz Urte , Bolger Niall , Shrout Patrick E. , Knoll Nina , Lüscher Janina 
Published: May 2023

AbstractCompanionship is related to better affect and relationship satisfaction, but few studies have examined both partners' perspectives over time and the link between companionship and health. In three intensive longitudinal studies (Study 1: 57 community couples; Study 2: 99 smoker–nonsmoker couples; Study 3: 83 dual‐smoker couples), both partners reported daily companionship, affect, relationship satisfaction, and a health behavior (smoking in Studies 2 and 3). We proposed a dyadic score model that focuses on the couple level for companionship as a dyadic predictor with considerable shared variance. On days with higher companionship, couples reported better affect and relationship satisfaction. When partners differed in companionship, they also differed in affect and relationship satisfaction. For smoking, a different picture emerged: Whereas smokers with nonsmoking partners smoked less on average with higher companionship, smokers with smoking partners smoked more on days with higher companionship. Findings show companionship as a consequential relationship construct deserving further study. Using the dyadic score model acknowledged both partners' perspectives on companionship. It demonstrated higher precision for detecting effects of partner averages in a dyadic predictor compared with traditional approaches, tests for effects of partner differences in a dyadic predictor and in outcome while maintaining the focus on the dyad.