bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome Sequence of the Cacao Pathogen Moniliophthora roreri Belonging to the Invasive A1B1 Mating Type: A Resource for Genomic Efforts in Cacao Pathology
Authors: Díaz-Valderrama Jorge R. , Rubio-Rojas Karol B. , Fernández Nadia B. , Kijpornyongpan Teeratas , Phillips-Mora Wilbert , Weil Clifford F. , Gribskov Michael , Aime M. Catherine 
Published: March 2023

We sequenced the genome of the cacao pathogen Moniliophthora roreri MCA2952, which harbors mating type A1B1 and is the first instance of the fungus identified in Mexico. This draft genome consists of 59.68 Mb on 254 scaffolds; it is 95.8% complete based on the analysis of Benchmarking Universal Single-Copy Orthologs (BUSCO v.2 with the Basidiomycota odb9 database), and it is by far the most contiguous genome generated for the genus Moniliophthora. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Co‐expression of diurnal and ultradian rhythms in the plasma metabolome of common voles (Microtus arvalis)
Authors: Psomas Andreas , Chowdhury Namrata R. , Middleton Benita , Winsky‐Sommerer Raphaelle , Skene Debra J. , Gerkema Menno P. , van der Veen Daan R. 
Published: March 2023

AbstractMetabolic rhythms include rapid, ultradian (hourly) dynamics, but it remains unclear what their relationship to circadian metabolic rhythms is, and what role meal timing plays in coordinating these ultradian rhythms in metabolism. Here, we characterized widespread ultradian rhythms under ad libitum feeding conditions in the plasma metabolome of the vole, the gold standard animal model for behavioral ultradian rhythms, naturally expressing ~2‐h foraging rhythms throughout the day and night. These ultradian metabolite rhythms co‐expressed with diurnal 24‐h rhythms in the same metabolites and did not align with food intake patterns. Specifically, under light–dark entrained conditions we showed twice daily entrainment of phase and period of ultradian behavioral rhythms associated with phase adjustment of the ultradian cycle around the light–dark and dark–light transitions. These ultradian activity patterns also drove an ultradian feeding pattern. We used a unique approach to map this behavioral activity/feeding status to high temporal resolution (every 90 min) measures of plasma metabolite profiles across the 24‐h light–dark cycle. A total of 148 known metabolites were detected in vole plasma. Supervised, discriminant analysis did not group metabolite concentration by feeding status, instead, unsupervised clustering of metabolite time courses revealed clusters of metabolites that exhibited significant ultradian rhythms with periods different from the feeding cycle. Two clusters with dissimilar ultradian dynamics, one lipid‐enriched (period = 3.4 h) and one amino acid‐enriched (period = 4.1 h), both showed co‐expression with diurnal cycles. A third cluster solely comprised of glycerophospholipids (specifically ether‐linked phosphatidylcholines) expressed an 11.9 h ultradian rhythm without co‐expressed diurnal rhythmicity. Our findings show coordinated co‐expression of diurnal metabolic rhythms with rapid dynamics in feeding and metabolism. These findings reveal that ultradian rhythms are integral to biological timing of metabolic regulation, and will be important in interpreting the impact of circadian desynchrony and meal timing on metabolic rhythms.
Preoperative Range of Motion in Extension May Influence Postoperative Cervical Kyphosis After Laminoplasty
Authors: Ren Hai-long , Shen Xing , Ding Ruo-ting , Cai Hai-Bo , Zhang Gong-liang 
Published: March 2023

Study Design.
Retrospective observational study.

Objective.
To investigate factors associated with cervical kyphosis after laminoplasty.

Summary of Background Data.
Many factors are reportedly associated with the deterioration of cervical curvature after laminoplasty, including cervical lordosis angle, cervical spine range of motion (ROM), T1 slope, and C2–7 sagittal vertical axis. Post-laminoplasty kyphosis or deterioration of cervical curvature is likely caused by multiple factors. There is currently no consensus on these issues.

Methods.
Data of patients treated with laminoplasty for degenerative cervical myelopathy at our institution during 2008–2018 were reviewed. The following variables were collected for each patient: age and sex; follow-up time; surgery involving C3 (yes or no); surgery involving C7 (yes or no); distribution of segments operated on; number of laminae operated on; flexion, extension, and total ROM; cervical lordotic angle; longitudinal distance index; curvature index; C2–7 sagittal vertical axis; and T1 slope. Logistic regression analysis was used to assess possible risk factors for postoperative kyphosis. Receiver operating characteristic curves were constructed to determine the cut-off values of risk factors.

Results.
The study cohort comprised 151 patients. Logistic regression analysis indicated that sex, number of laminae operated on, and preoperative extension ROM were significantly associated with postoperative cervical kyphosis (P<0.05). There was significantly greater postoperative kyphosis in women than in men; the more segments operated on, the greater the risk of postoperative kyphosis, and the larger the preoperative extension ROM, the lower the risk of post-laminoplasty kyphosis. Receiver operating curve analysis showed that the cut-off value for preoperative extension ROM is 22.1°.

Conclusion.
Preoperative extension ROM may be associated with the development of postoperative kyphosis. The cut-off value of preoperative extension ROM that suggested the prospect of postoperative kyphosis in our sample was 22.1°.
Are We Considering the Whole Patient? The Impact of Physical and Mental Health on the Outcomes of Spine Care
Authors: Turcotte Justin J. , Baxter Samantha , Pipkin Karen , Patton Chad M. 
Published: March 2023

Study Design.
Retrospective, observational.

Objective.
To evaluate the influence of baseline health status on the physical and mental health (MH) outcomes of spine patients.

Summary of Background Data.
Spine conditions can have a significant burden on both the physical and MH of patients. To date, few studies have evaluated the outcomes of both dimensions of health, particularly in nonoperative populations.

Materials and Methods.
At their first visit to a multidisciplinary spine clinic, 2668 nonoperative patients completed the Patient-reported Outcomes Measurement Information System-Global Health (PROMIS-GH) instrument and a questionnaire evaluating symptoms and goals of care. Patients were stratified by their baseline percentile score of the MH and physical health (PH) components of the PROMIS-GH. Four groups of patients were compared based on the presence or absence of bottom quartile PH or MH scores. The primary end point was the achievement of a minimal clinically important difference (MCID) on the MH or PH components at follow-up. Multivariate regression assessed the predictors of MCID achievement.

Results.
After controlling for demographics, symptoms, and goals, each 1-point increase in baseline PROMIS-GH mental score reduced the odds of achieving MH MCID by 9.0% (P<0.001). Conversely, each 1-point increase in baseline GH-physical score increased the odds of achieving MCID by 4.5% (P=0.005). Each 1-point increase in baseline GH-physical score reduced the odds of achieving PH MCID by 12.5% (P<0.001), whereas each 1-point increase in baseline GH-mental score increased the odds of achieving MCID by 5.0% (P<0.001).

Conclusions.
Spine patients presenting with the lowest levels of physical or MH were most likely to experience clinically significant improvement in those domains. However, lower levels of physical or mental health made it less likely that patients would experience significant improvement in the alternative domain. Physicians should evaluate and address the complex spine population holistically to maximize improvement in both physical and mental health status.
One-year Postoperative Radiographic and Patient-reported Outcomes Following Cervical Deformity Correction Are Not Affected by a Short-term Unplanned Return to the OR
Authors: Fourman Mitchell S. , Lafage Renaud , Ames Christopher , Smith Justin S. , Passias Peter G. , Shaffrey Christopher I. , Mundis Gregory , Protopsaltis Themistocles , Gupta Munish , Klineberg Eric O. , Bess Shay , Lafage Virginie , Kim Han Jo ,   on behalf of the International Spine Study Group
Published: March 2023

Study Design.
Retrospective analysis of a prospectively collected multicenter database.

Objective.
The objective of this study was to assess the radiographic and health-related quality of life (HRQoL) impact of a short-term (<1 y) return to the operating room (OR) after adult cervical spine deformity (ACSD) surgery.

Summary of Background Data.
Returns to the OR within a year of ACSD correction can be particularly devastating to these vulnerable hosts as they often involve compromise of the soft tissue envelope, neurological deficits, or hardware failure. This work sought to assess the impact of a short-term reoperation on 1-year radiographic and HRQoL outcomes.

Materials and Methods.
Patients operated on from January 1, 2013, to January 1, 2019, with at least 1 year of follow-up were included. The primary outcome was a short-term return to the OR. Variables of interest included patient demographics, Charlson Comorbidity Index, HRQoL measured with the modified Japanese Orthopaedic Association), Neck Disability Index, and EuroQuol-5D Visual Analog Scale (EQ-5D VAS) and radiographic outcomes, including T1 slope, C2–C7 sagittal Cobb angle, T1 slope–Cobb angle, and cervical sagittal vertical axis. Comparisons between those who did versus did not require a 1-year reoperation were performed using paired t tests. A Kaplan-Meier survival curve was used to estimate reoperation-free survival up to 2 years postoperatively.

Results.
A total of 121 patients were included in this work (age: 61.9±10.1 yr, body mass index: 28.4±6.9, Charlson Comorbidity Index: 1.0±1.4, 62.8% female). A 1-year unplanned return to the OR was required for 28 (23.1%) patients, of whom 19 followed up for at least 1 year. Indications for a return to the OR were most commonly for neurological complications (5%), infectious/wound complications (5.8%), and junctional failure (6.6%) No differences in demographics, comorbidities, preoperative or 1-year postoperative HRQoL, or radiographic outcomes were seen between operative groups.

Conclusion.
Reoperation <1 year after ACSD surgery did not influence 1-year radiographic outcomes or HRQoL.
Changes in Corneal Clouding Over Time in Patients With Mucopolysaccharidosis
Authors: McGrath Orlaith , Sornalingam Krishanthy , Aslam Tariq , Ashworth Jane 
Published: March 2023

Purpose:
Mucopolysaccharidoses (MPSs) are a rare group of lysosomal storage disorders characterized by the accumulation of incompletely degraded glycosaminoglycans (GAGs) in multiple organ systems, including the eye. Visual loss occurs in MPS predominantly due to corneal clouding. Despite the success of enzyme replacement therapy (ERT) and hematopoietic stem cell transplantation (HSCT) in improving many systemic manifestations of MPS, less is known about their effect on corneal clouding. This study prospectively analyses the effect of both ERT and HSCT on corneal clouding using objective measures over time.

Methods:
This is a prospective longitudinal observational study. Corneal clouding was assessed in each participant using slitlamp, digital slit-lamp photographs, and an iris camera (Corneal Opacification Measure [COM] and the Pentacam system).

Results:
Data were collected for 65 participants: 39 MPS I (Hurler), 5 MPS II (Hunter), 12 MPS IV (Morquio), and 9 MPS VI (Maroteaux–Lamy). Follow-up data are available for 45 participants (29 MPS I, 3 MPS II, 6 MPS IV, and 7 MPS VI).

Conclusions:
This study found corneal clouding to be stable in most participants with MPS I, II, IV, and VI over a follow-up period of 5 to 75 months (median of 30 months) when measured with clinical corneal grading systems, graded digital slit-lamp images, and iris camera COMs. For those with Pentacam densitometry measures, there was a progression of corneal clouding, on average, in those with MPS I and MPS VI. There was no apparent difference in progression of corneal clouding between patients who were on ERT, HSCT, or no treatment.
A Literature Review: Violence and Aggression in Neuroscience Nursing
Authors: Woon Caroline 
Published: March 2023

ABSTRACT

BACKGROUND: Violence and aggression is commonly encountered in nursing worldwide and is an increasing concern, although it is largely underreported by staff. Violence and aggression can take many forms, from verbal and physical abuse to sexual assault. This study aims to define agitation, violence, and aggression and to explore the prevalence of violence and aggression among neuroscience patients. This review also examines why violence and aggression occurs for neuroscience patients and to determine the effects on the patients, the environment, and the nursing staff. METHODS: A review of articles was conducted using CINAHL, PubMed, the Cochrane Database, and Google Scholar between 2012 and 2022. DISCUSSION: Agitation can escalate to violence and aggression. The reasons a neuroscience patient may become agitated are multifactorial. An injury to the brain may not cause agitation; however, the effect on the frontal lobe, hypothalamus, and hippocampus may cause a lack of self-control, impulsivity, an inability to control emotions, and an uncontrolled release of hormones, leading to a heightened sympathetic response. The effects of violence and aggression can be detrimental to the patient and include isolation, increased sedation, reduced observations, and even death. The effects on the nurse are profound including a decline in productivity at work, an increased risk of drug errors, and posttraumatic stress disorder or burnout as longer-term consequences. CONCLUSION: Violence and aggression is commonly experienced within neuroscience nursing, and the contributing factors are multifactorial. The effects for the patients and staff can be profound, and this is why prevention of agitation is fundamental to ensure the safety and retention of nursing staff.
Contemporary adult gerontology acute care nurse practitioner (AGACNP) practice: Findings from the 2020 American Association of Critical Care Nurses AGACNP practice analysis survey
Authors: Jackson Kristopher , McCauley Paula , Konick-McMahan Joanne , Mirza Cyrus 
Published: March 2023

ABSTRACT

Background:
National standards for nurse practitioner licensure require certification programs to conduct practice analyses to ensure that certified nurse practitioners possess the necessary knowledge for entry-level practice. The practice analysis for the American Association of Critical Care Nurses Certification Corporation (AACN Cert Corp) adult-gerontology acute care nurse practitioner (AGACNP) credential is performed every five years by the AACN Certification Corporation.

Purpose:
The AACN Cert Corp conducted a practice analysis to confirm that current AGACNP practice is reflected in the ACNPC-AG test plan, and the examination is congruent with 2008 consensus model guidelines. This work describes findings from the 2020 AACN Cert Corp practice analysis and changes in AGACNP practice and academic preparation based on the survey data.

Method: :
In 2020, AACN Cert Corp volunteer subject matter experts (SMEs) developed a survey of practice activities and competencies relevant to AGACNP practice. Patient care activities and competencies were rated by AGACNP respondents for criticality and frequency. AACN SMEs reviewed criticality and frequency ratings to determine the patient care problems, skills/procedures, and competencies to include in the updated AACN Cert Corp ACNPC-AG test plan.

Results:
The 2020 AGACNP practice analysis survey and subsequent review resulted in the retention of 33 skills and procedures, 165 patient care problems, and all national competencies in the final ACNPC-AG test plan.

Conclusions and Implications:
The 2020 AACN Cert Corp AGACNP practice analysis survey describes possible changes in AGACNP practice and academic preparation that have occurred since the 2016 survey, findings that may be associated with the ongoing COVID-19 pandemic.
Local Infiltration of Tranexamic Acid in Breast Reduction for Symptomatic Macromastia
Authors: Nichols Georgina , Andrade Erika , Gregoski Mathew , Herrera Fernando A. , Armstrong Milton B. 
Published: March 2023

Background
With a surge of tranexamic acid (TXA) use in the plastic surgery community and a constant demand for breast reduction for symptomatic macromastia, questions about the benefits and risks emerge. The aims of this study are to evaluate and compare outcomes of patients undergoing breast reduction while receiving local TXA as opposed to standard procedure without TXA and to assess intraoperative bleeding and operative time.

Methods
A retrospective review of breast reductions at a single institution from June 2020 to December 2021 was performed. The breast was infiltrated with tumescent solution at the time of surgery, with or without TXA. The population was divided into 2 groups: the TXA receiving group and tumescent only group. Demographics, intraoperative bleeding, operative time, complications, and drain duration were compared between groups. T test and χ2 test analyses were performed on IBM SPSS.TM

Results
A total of 81 patients and 162 breasts were included. Mean age among patients was 30 ± 13.44 years. Mean SN-N distance was 32.80 ± 3.62 cm. Average resected breast specimen weight was 903.21 ± 336.50 g. Mean operating room time was 159 minutes. Intraoperative blood loss and operative time were not statistically different between groups (P = 0.583 and P = 0.549, respectively). T-junction dehiscence was lower in the TXA group (P = 0.016). Incidence of suture granulomas was lower in the TXA group (P = 0.05). Drain duration was statistically significantly higher in the TXA group (P = 0.033).

Conclusions
No decreases in intraoperative blood loss, operative time, or hematoma were seen after local administration of TXA during breast reduction. The rate of overall complications was not increased by using TXA, and incidence of T-junction dehiscence was lower in the TXA group lending to TXA's relatively safe profile. More research is necessary to further elucidate the TXA-related benefits in standard breast reductions.
Using an Expanded Cervical Flap With an Overlapping Tissue Expansion Technique to Resurface Middle and Lower Facial Defects
Authors: Luo Si-Si , Li Yang-Qun 
Published: March 2023

Background:
Extensive facial burn scars are a tragedy for patients and often pose a great challenge to surgeons because of the high esthetic and functional demands. For patients with healthy skin in the neck region, a cervical flap is highly recommended for facial resurfacing; however, the skin on the midline of the neck often needs more expansion than that on either side, especially for the treatment of large facial defects. The sufficient longitudinal soft tissue in the anterior neck ensures a normal neck shape as well as a normal range of cervical extension, rotation, and lateral flexion. To overcome this, we developed an expanded cervical flap with an overlapping tissue expansion technique to gain more length centrally.

Methods:
First, 2 tissue expanders were embedded in the anterior neck region overlapping each other at the midline of the neck. After adequate inflation of the expander, the expanded flap was dissected and rotated to repair defects in the middle and lower face. The anchor position of the flap was placed on the horizontal line of the thyroid cartilage to restore the cervicomental angle.

Results:
Sixteen patients were treated with this method in this single-center study. All defects affected the middle and lower face, with an area ranging from 135 to 185 cm2, and were caused by a massive facial burn. Among them, 12 patients suffered ectropion of the lower lip, 3 suffered limited mouth opening due to scar contraction, and one patient had a cervicomental adhesion. The area of the expanded flap was approximately 163 to 266 cm2. The average period of expansion was 89.5 days. Patients were followed up after the operation, with the follow-up period ranging from 6 to 12 months. In all cases, good defect coverage was achieved, with primary closure of the donor sites and a good postoperative cervical configuration.

Conclusion:
We conclude that the expanded cervical flap with the overlapping tissue expansion technique proved to be a reliable method for facial skin reconstruction with functional and aesthetic improvement.
New Features Surrounding the Cdc42-Ste20 Module that Regulates MAP Kinase Signaling in Yeast
Authors: González Beatriz , Mirzaei Mahnoosh , Basu Sukanya , Prabhakar Aditi , Cullen Paul J. 
Published: March 2023

ABSTRACTMitogen-activated protein kinase (MAPK) pathways regulate multiple cellular responses, including the response to stress and cell differentiation, and are highly conserved across eukaryotes from yeast to humans. In yeast, the canonical activation of several MAPK pathways includes the interaction of the small GTPase Cdc42p with the p21-activated kinase (PAK) Ste20p. We recently found that the active conformation of Cdc42p is regulated by turnover, which impacts the activity of the pathway that regulates filamentous growth (fMAPK). Here, we show that Ste20p is turned over by the 26S proteasome. Ste20p was stabilized when bound to Cdc42p, presumably to sustain MAPK pathway signaling. Ste20p is a major conduit by which signals flow through the fMAPK pathway; however, by genetic approaches we also identified a Ste20p-independent branch of the fMAPK pathway. Ste20p-dependent signaling required the 14-3-3 proteins, Bmh1p and Bmh2p, while Ste20p-independent signaling required the fMAPK pathway adaptor and Cdc42p-interacting protein, Bem4p. Ste20p-independent signaling was inhibited by one of the GTPase-activating proteins for Cdc42p in the fMAPK pathway, Rga1p, which also dampened basal but not active fMAPK pathway activity. Finally, the polarity adaptor and Cdc42p-interacting protein, Bem1p, which also regulates the fMAPK pathway, interacts with the tetra-span protein Sho1p, connecting a sensor at the plasma membrane to a protein that regulates the GTPase module. Collectively, these data reveal new regulatory features surrounding a Rho-PAK module that may extend to other pathways that control cell differentiation.