bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevention of intrusion of natural teeth surrounded by freestanding implants
Authors: Rostami Arash Mike , Feldman Sylvan , Zeiger Diana N. 
Published: January 2023

AbstractIntrusion is frequently observed as a complication associated with tooth‐implant‐supported prostheses: the natural tooth that serves as an abutment moves apically and out of occlusion. However, intrusion of teeth proximal to implants has yet to be reported in the literature. In this manuscript, three presentations of tooth intrusion proximal to implants are reported; potential causes of the phenomenon are discussed; and a potential treatment for patients experiencing this condition is presented. Intrusion of teeth surrounded by freestanding implants is a rare phenomenon, a cause of which may be excessively tight proximal contacts that are below the height of contour of adjacent implants. Alleviating these contacts enables the intruded tooth to erupt back and should be considered a viable treatment option.
Needleless inhaled anesthesia with sevoflurane: Advantages of a simplified approach for children with spinal muscular atrophy undergoing intrathecal administration of nusinersen
Authors: Salerno Annalisa , Picconi Enzo , Genovese Orazio , Piastra Marco , Pulitanò Silvia M. , Tosi Federica , Mancino Aldo , Pane Marika , De Sanctis Roberto , Carlini Debora , Mercuri Eugenio M. , Conti Giorgio 
Published: January 2023

AbstractBackgroundIntrathecal nusinersen administration, a fundamental step in the treatment of spinal muscular atrophy, is challenging in children.AimsThis retrospective monocentric analysis of prospectively collected data evaluated the feasibility of needleless general anesthesia exclusively with sevoflurane, without imaging guidance, for children undergoing nusinersen administration in a 24‐month period.MethodsClinical data included demographics, type of spinal muscular atrophy, presence and severity of scoliosis. Primary outcome was defined by the number of predefined sentinel adverse events related to anesthesia. Secondary outcomes were assessed by duration of the procedure, number of lumbar puncture attempts, and number of failures. Other measures included number and type of moderate, minor and minimal adverse events, as well as number and type of puncture‐related adverse events.Results116 patients (mean age: 8.7 (SD 6.9) years; with scoliosis: 49.1%) underwent 250 lumbar punctures; two cases of prolonged desaturation, considered as sentinel adverse events, (0.8%) were recorded during anesthesia (primary outcome). None of the patients underwent orotracheal intubation nor required an unplanned admission in the Pediatric Intensive Care Unit. No patient required an unplanned or prolonged hospitalization after the procedure. Mean number of puncture attempts was 1.6 (SD 1.3), and mean duration of the procedure was 14.1 (SD 8.3) minutes. No failure in the drug administration occurred (secondary outcomes).ConclusionIn this single‐center experience, needleless general anesthesia with inhaled sevoflurane without imaging guidance has been shown to be feasible for children with spinal muscular atrophy undergoing lumbar puncture for nusinersen administration.
Fusarium falciforme and Fusarium suttonianum cause root rot of melon in Brazil
Authors: Silva Shamyra G. A. , Costa Marileide M. , Cardoso Acleide M. S. , Nascimento Luan V. , Barroso Karol A. , Nunes Glauber H. S. , Pfenning Ludwig H. , Ambrósio Márcia M. Q. 
Published: January 2023

AbstractThe north‐east of Brazil is one of the most important melon‐producing regions in the world. Crop yield is often reduced by the occurrence of a root rot disease, caused by Fusarium species. The present study aimed to characterize a collection of 31 isolates with morphological markers of F. solani obtained from melon plants with symptoms of root rot by phylogenetic analysis of the barcode regions EF‐1α and RPB2, and to verify their pathogenicity to melon plantlets. Phylogenetic analysis showed that 29 isolates grouped with reference material of Fusarium falciforme (FSSC 3 + 4) and two with F. suttonianum (FSSC 20). The pathogenicity test showed that isolates of both species cause root rot in melon plants, with no significant difference of virulence between isolates and species with both methodologies used (infested toothpick and infested rice). Melon plants expressed first symptoms 15 days after inoculation, showing yellowing and wilting, with later tipping and rot. To our knowledge, this is the first report of the occurrence of these species causing root rot in muskmelon in Brazil. Our results support monitoring of causal agents of melon fusarioses and will be useful for breeding programmes in the search for plant material with resistance to Fusarium rot.
Synergistic interaction of two bHLH transcription factors positively regulates artemisinin biosynthetic pathway in Artemisia annua L.
Authors: Mohammad  , Hurrah Ishfaq Majid , Kumar Amit , Abbas Nazia 
Published: January 2023

AbstractThe wonder drug artemisinin, a sesquiterpene lactone endoperoxide from Artemisia annua is the million‐dollar molecule required to curb the deadliest disease, Malaria. One of the major challenges even today is to increase the concentration of artemisinin within plants. The transcription factors are important regulators of plant secondary metabolites and have the potential to regulate key steps or the whole biosynthetic pathway. In this study, we have identified and characterised two bHLH transcription factors (Aa6119 and Aa7162) from A. annua. Both the transcription factors turned out to be transcriptionally active and nuclear‐localised typical bHLH proteins. In our study, we found that Aa6119 specifically binds to the E‐box element present on the promoter of artemisinin biosynthetic gene, AMORPHA‐4,11‐DIENE SYNTHASE (ADS). The protein‐DNA interaction confirmed by Yeast one‐hybrid assay was specific as Aa6119 was unable to bind to the mutated E‐boxes of ADS. Further, Aa6119 interacted physically with Aa7162, which was confirmed in vitro by Yeast two‐hybrid assay and in vivo by Bimolecular Fluorescent complementation assay. Our quantitative expression studies have confirmed that Aa6119 and Aa7162 act synergistically in the regulation of artemisinin biosynthetic and trichome developmental genes. The higher accumulation of artemisinin content in the transient co‐transformed transgenic plants than in the individual over‐expression transgenic plants has further validated that Aa6119 and Aa7162 act positively and synergistically to regulate artemisinin accumulation.
Evaluation of the safety and efficacy of a fractional picosecond 1064 nm laser for post‐acne erythema in adult Chinese patients
Authors: Jia Ximeng , Zheng Lingyu , Fang Lin , Zhang Ling , Wu Ying , Yang Yang , Feng Yongqiang , Huang Lvping 
Published: January 2023

AbstractObjectiveTo evaluate the efficacy and safety of fractional 1064 nm picosecond Nd:YAG laser (FPNYL) in the treatment of post‐acne erythema (PAE) of adult Chinese.Materials and methodsA total of 22 patients received 1 session of treatment and were followed up at the eighth week. Primary outcomes were measured by the Clinician erythema assessment scale (CEAS). Secondary outcomes included a global aesthetic improvement scale (GAIS) and patients’ assessment of satisfaction on a five‐point scale. Pain scores and adverse effects were also evaluated.ResultsTwenty‐two patients with Fitzpatrick skin types III and IV were enrolled in the study and completed all treatments and follow‐up visits. The mean CEAS scores fell from 2.74 ± 0.80 to 1.95 ± 0.75 (p < 0.05). The mean GAIS of PAE improvement was 2.46 ± 0.68. Erythema percentile scores by VISIA increased from 32.63 ± 7.0 to 45.75 ± 11.45 (t = 5.442, p = 0). The patient satisfaction score was 1.86 ± 1.17. The pain scores were 3.27 ± 1.17 for the FPNYL treatment (varied from 2 to 6). There were moderate erythema and oedema, which last for 3.84 ± 0.78 days. There were overall 68.18% (15/22) patients who felt pruritus in different degrees and 27.27% patients who encountered acne eruptions (white head type). No scar, hyperpigmentation or hypopigmentation was found.ConclusionTreatment with fractional 1064 nm picosecond Nd:YAG laser is effective and safe for PAE of Chinese patients.
Social bonds are related to health behaviors and positive well-being globally
Authors: Tunçgenç Bahar , van Mulukom Valerie , Newson Martha 
Published: January 2023

At times of turmoil, such as during disasters, social crises, or pandemics, our social bonds can be key to receiving support and gaining certainty about the right course of action. In an analysis combining two global datasets (
N
= 13,264) collected during the first wave of the COVID-19 pandemic, this study examined how social bonds with close social circles (i.e., family and friends) and extended groups (i.e., country, government, and humanity) relate to engagement in health behaviors and psychological well-being. Results revealed that only family bonding was associated with self-reported engagement in health behaviors. Being strongly bonded with both close circles and extended groups predicted less anxiety and depression and better well-being, particularly for those who were bonded with more groups. These findings highlight that close and extended social bonds offer different sources of support and direction during the most challenging of circumstances and that continuous investment is needed to forge and maintain both.
Does Nrf2 Play a Role of a Master Regulator of Mammalian Aging?
Authors: Zinovkin Roman A. , Kondratenko Natalia D. , Zinovkina Ludmila A. 
Published: January 2023

Abstract
For a long time Nrf2 transcription factor has been attracting attention of researchers investigating phenomenon of aging. Numerous studies have investigated effects of Nrf2 on aging and cell senescence. Nrf2 is often considered as a key player in aging processes, however this needs to be proven. It should be noted that most studies were carried out on invertebrate model organisms, such as nematodes and fruit flies, but not on mammals. This paper briefly presents main mechanisms of mammalian aging and role of inflammation and oxidative stress in this process. The mechanisms of Nrf2 activity regulation, its involvement in aging and development of the senescence-associated secretory phenotype (SASP) are also discussed. Main part of this review is devoted to critical analysis of available experimental data on the role of Nrf2 in mammalian aging.
Multifaceted Nothobranchius
Authors: Bulavkina Elizaveta V. , Kudryavtsev Alexander A. , Goncharova Margarita A. , Lantsova Margarita S. , Shuvalova Anastasija I. , Kovalev Maxim A. , Kudryavtseva Anna V. 
Published: January 2023

Abstract
Annual killifish of the genus Nothobranchius are seeing a rapid increase in scientific interest over the years. A variety of aspects surrounding the egg-laying Cyprinodontiformes is being extensively studied, including their aging. Inhabiting drying water bodies of Africa rarely allows survival through more than one rainy season for the Nothobranchius populations. Therefore, there is no lifespan-related bias in natural selection, which has ultimately led to the decreased efficiency of DNA repair system. Aging of the Nothobranchius species is studied both under normal conditions and under the influence of potential geroprotectors, as well as genetic modifications. Most biogerontological studies are conducted using the species Nothobranchiusfurzeri (GRZ isolate), which has a lifespan of 3 to 7 months. However, the list of model species of Nothobranchius is considerably wider, and the range of advanced research areas with their participation extends far beyond gerontology. This review summarizes the most interesting and promising topics developing in the studies of the fish of Nothobranchius genus. Both classical studies related to lifespan control and rather new ones are discussed, including mechanisms of diapause, challenges of systematics and phylogeny, evolution of sex determination mechanisms, changes in chromosome count, occurrence of multiple repeated DNA sequences in the genome, cognitive and behavioral features and social stratification, as well as methodological difficulties in working with Nothobranchius.
Innate Immunity and Phenoptosis
Authors: Chernyak Boris V. , Lyamzaev Konstantin G. 
Published: January 2023

Abstract
The hypothesis is proposed that activation of innate immunity is the primary mechanism of phenoptosis (programmed death of an organism). In support of the hypothesis, we discuss (i) the data on active release of signaling molecules from the cell producing excessive inflammation; (ii) the data on contribution of mitochondrial production of reactive oxygen species to immune response.
Fluorescence Polarization Immunoassay of Human Lactoferrin in Milk Using Small Single-Domain Antibodies
Authors: Mukhametova Lilia I. , Eremin Sergei A. , Arutyunyan Dmitrii A. , Goryainova Oksana S. , Ivanova Tatiana I. , Tillib Sergei V. 
Published: January 2023

Abstract
Due to its unique structure and properties, human breast milk lactoferrin (hLF) has many nutritional and health-promoting functions in infants, including protection against inflammation and bacterial infections. The lack of LF in breastmilk or formula can result in the weakening of the infant’s immune system. Noncompetitive polarization fluorescence immunoassay (FPIA) is a promising method for hLF quantification in milk and dairy products, which does not require the separation of the bound and free protein and allows to avoid time-consuming sample preparation. The use of fluorescently labeled single-domain camelid antibodies (nanobodies) for protein recognition in FPIA makes it possible to quantify relatively large antigens, in particular, hLF. In this work, we used previously obtained fluorescein isothiocyanate (FITC)-conjugated anti-hLF5 and anti-hLF16 nanobodies, which selectively recognized two different human lactoferrin epitopes, but did not bind to goat lactoferrin. The kinetics of hLF interaction with the FITC-labeled nanobodies was studied. The dissociation constant (KD) for the anti-LF5 and anti-LF16 nanobodies was 3.2 ± 0.3 and 4.9 ± 0.4 nM, respectively, indicating the high-affinity binding of these nanobodies to hLF. We developed the FPIA protocol and determined the concentration of FITC-labeled anti-hLF5 and anti-hLF16 nanobodies that provided the optimal fluorescence signal and stable fluorescence polarization value. We also studied the dependence of fluorescence polarization on the hLF concentration in the noncompetitive FPIA with FITC-anti-hLF5 nanobody. The detection limit for hLF was 2.1 ± 0.2 µg/ml and the linear range for determining the hLF concentration was 3-10 µg/ml. FPIA is commonly used to assay low-molecular-weight substances; however, the use of fluorescently labeled nanobodies allows quantification of high-molecular-weight proteins. Here, we demonstrated that FPIA with fluorescently labeled nanobodies can be used for hLF quantification in milk.
Spatiotemporal dynamics of malaria in Zanzibar, 2015–2020
Authors: Bisanzio Donal , Lalji Shabbir , Abbas Faiza B , Ali Mohamed H , Hassan Wahida , Mkali Humphrey R , Al-Mafazy Abdul-wahid , Joseph Joseph J , Nyinondi Ssanyu , Kitojo Chonge , Serbantez Naomi , Reaves Erik , Eckert Erin , Ngondi Jeremiah M , Reithinger Richard 
Published: January 2023

BackgroundDespite high coverage of malaria interventions, malaria elimination in Zanzibar remains elusive, with the annual number of cases increasing gradually over the last 3 years.ObjectiveThe aims of the study were to (1) assess the spatiotemporal dynamics of malaria in Zanzibar between 2015 and 2020 and (2) identify malaria hotspots that would allow Zanzibar to develop an epidemiological stratification for more effective and granular intervention targeting.MethodsIn this study, we analysed data routinely collected by Zanzibar’s Malaria Case Notification (MCN) system. The system collects sociodemographic and epidemiological data from all malaria cases. Cases are passively detected at health facilities (ie, primary index cases) and through case follow-up and reactive case detection (ie, secondary cases). Analyses were performed to identify the spatial heterogeneity of case reporting at shehia (ward) level during transmission seasons.ResultsFrom 1 January 2015 to 30 April 2020, the MCN system reported 22 686 index cases. Number of cases reported showed a declining trends from 2015 to 2016, followed by an increase from 2017 to 2020. More than 40% of cases had a travel history outside Zanzibar in the month prior to testing positive for malaria. The proportion of followed up index cases was approximately 70% for all years. Out of 387 shehias, 79 (20.4%) were identified as malaria hotspots in any given year; these hotspots reported 52% of all index cases during the study period. Of the 79 hotspot shehias, 12 were hotspots in more than 4 years, that is, considered temporally stable, reporting 14.5% of all index cases.ConclusionsOur findings confirm that the scale-up of malaria interventions has greatly reduced malaria transmission in Zanzibar since 2006. Analyses identified hotspots, some of which were stable across multiple years. Malaria efforts should progress from a universal intervention coverage approach to an approach that is more tailored to a select number of hotspot shehias.
EasyTBSA as a method for calculating total body surface area burned: a validation study
Authors: Colson Cindy D , Alberto Emily C , Milestone Zachary P , Batra Nikita , Salvador Tyler , Fooladi Hadi , Cleary Kevin , Izem Rima , Burd Randall S 
Published: January 2023

BackgroundCurrent methods of burn estimation can lead to incorrect estimates of the total body surface area (TBSA) burned, especially among injured children. Inaccurate estimation of burn size can impact initial management, including unnecessary transfer to burn centres and fluid overload during resuscitation. To address these challenges, we developed a smartphone application (EasyTBSA) that calculates the TBSA of a burn using a body-part by body-part approach. The aims of this study were to assess the accuracy of the EasyTBSA application and compare its performance to three established methods of burn size estimation (Lund-Browder Chart, Rule of Nines and Rule of Palms).MethodsTwenty-four healthcare providers used each method to estimate burn sizes on moulaged manikins. The manikins represented different ages (infant, child and adult) with different TBSA burns (small <20%, medium 20%–49% and large >49%). We calculated the accuracy of each method as the difference between the user-estimated and actual TBSA. The true value of the complete body surface area of the manikins was obtained by three-dimensional scans. We used multivariable modelling to control for manikin size and method.ResultsAmong all age groups and burn sizes, the EasyTBSA application had the greatest accuracy for burn size estimation (−0.01%, SD 3.59%) followed by the Rule of Palms (3.92%, SD 10.71%), the Lund-Browder Chart (4.42%, SD 5.52%) and the Rule of Nines (5.05%, SD 6.87%).ConclusionsThe EasyTBSA application may improve the estimation of TBSA compared with existing methods.
Reconstructive endovascular treatment for basilar artery trunk aneurysms: complications and clinical and angiography outcomes
Authors: Zhong Weiying , Zhang Tongfu , Su Chenran , Zhou Donglin , Zhuang Jianfeng , Li Maogui , Xu Yangyang , Liu Ming , Zhang Mingxiang , Wang Yunyan , Wang Donghai , Su Wandong 
Published: January 2023

BackgroundBasilar artery trunk aneurysms (BTAs) are rare intracranial aneurysms. We aim to investigate the procedural complications and clinical and angiographic outcomes of BTAs treated with reconstructive endovascular treatment (EVT).MethodsWe retrospectively reviewed the data of 111 patients with BTAs who underwent reconstructive EVT during 2013–2022. The factors associated with procedural complications and clinical and angiographic outcomes were analyzed.ResultsThe study included 81 men and 30 women (median age 60 years). Overall, 26 (23.4%) cases presented with subarachnoid hemorrhage and 85 (76.6%) presented with unruptured aneurysms. Periprocedural ischemic and hemorrhagic complications occurred in 29 (26.1%) and 4 (3.6%) cases, respectively. The rate of favorable clinical outcomes was 83.8% (92/111) and the mortality rate was 14.4% (16/111). Angiographic follow-up data were available for 77/95 (81.1%) survivors; 57 (74.0%) and 20 (26%) aneurysms exhibited complete and incomplete obliteration, respectively. Old age, high Hunt and Hess grades (IV–V), hemorrhagic complications, and increased aneurysm size were independent risk factors for unfavorable clinical outcomes (p<0.05). Increased aneurysm size and incomplete aneurysm occlusion on immediate angiography were independent risk factors for incomplete occlusion during follow-up (p<0.05).ConclusionReconstructive EVTs are a feasible and effective treatment for BTAs but are associated with a high risk of ischemic and hemorrhagic complications and a high mortality rate. Larger aneurysms may predict unfavorable clinical outcomes and aneurysm recurrence during follow-up. Hemorrhagic complications may predict unfavorable clinical outcomes, whereas immediate complete aneurysm occlusion may predict total occlusion during follow-up.
Selberg Zeta function and hyperbolic Eisenstein series
Authors: Falliero Thérèse 
Published: January 2023

Let [Formula: see text] be a Fuchsian group acting on the hyperbolic upper half-plane [Formula: see text], such that [Formula: see text] is a geometrically finite Riemann surface with respect to the natural hyperbolic metric induced from [Formula: see text]. If [Formula: see text] is hyperbolic then following [J. Kudla and S. Millson, Harmonic differentials and closed geodesics on a Riemann surface, Invent. Math. 54 (1979) 193–211; J. D. Fay, Fourier coefficients of the resolvent for a Fuchsian group, J. Reine Angew. Math. 293 (1977) 143–203], there is a corresponding hyperbolic Eisenstein series. In this paper, we study the limiting behavior of hyperbolic Eisenstein series on a degenerating family of geometrically finite hyperbolic surfaces. In particular, we give a partial lightening to a question of Ji, concerning the approximation of Eisenstein series during degeneration (see Proposition 5.2 and Theorem 5.2).
Collagens in Cancer: Structural Regulators and Guardians of Cancer Progression
Authors: De Martino Daniela , Bravo-Cordero Jose Javier 
Published: January 2023

Abstract
Collagen is one of the most abundant proteins in animals and a major component of the extracellular matrix (ECM) in tissues. Besides playing a role as a structural building block of tissues, collagens can modulate the behavior of cells, and their deregulation can promote diseases such as cancer. In tumors, collagens and many other ECM molecules are mainly produced by fibroblasts, and recent evidence points toward a role of tumor-derived collagens in tumor progression and metastasis. In this review, we focus on the newly discovered functions of collagens in cancer. Novel findings have revealed the role of collagens in tumor dormancy and immune evasion, as well as their interplay with cancer cell metabolism. Collagens could serve as prognostic markers for patients with cancer, and therapeutic strategies targeting the collagen ECM have the potential to prevent tumor progression and metastasis.