bluuebunny/crossref_metadata_2025_split huggingface dataset:
Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma
Authors: Guo Weitang , Wang Xin , Lu Bing , Yu Jiaming , Xu Mingxian , Huang Renxuan , Chen Mingzhe , Yang Meiling , Zhao Wei , Zou Changye 
Published: February 2023

Abstract
Osteosarcoma (OS) is a common type of bone tumor for which there has been limited therapeutic progress over the past three decades. The prevalence of transcriptional addiction in cancer cells emphasizes the biological significance and clinical relevance of super-enhancers. In this study, we found that Max-like protein X (MLX), a member of the Myc-MLX network, is driven by super-enhancers. Upregulation of MLX is predictive of poor prognosis in osteosarcoma. Knockdown of MLX impairs growth and metastasis of osteosarcoma in vivo and in vitro. Transcriptomic sequencing has revealed that MLX is involved in various metabolic pathways (e.g., lipid metabolism) and is capable of inducing metabolic reprogramming. More specifically, MLX regulates the glutamate/cystine antiporter SLC7A11 to promote extracellular cysteine uptake required for the biosynthesis of the essential antioxidant GSH, thereby detoxifying reactive oxygen species (ROS) and maintaining the redox balance of osteosarcoma cells. Importantly, sulfasalazine, an FDA-approved anti-inflammatory drug, can inhibit SLC7A11, disrupt redox balance, and induce massive ferroptosis, leading to impaired tumor growth in vivo. Taken together, this study reveals a novel mechanism whereby super-enhancer-driven MLX positively regulates SLC7A11 to meet the alleviated demand for cystine and maintain the redox balance, highlighting the feasibility and clinical promise of targeting SLC7A11 in osteosarcoma.
Impact of Topographic Localization of Corneal Ectasia on the Outcomes of Deep Anterior Lamellar Keratoplasty Employing Large (9 mm) Versus Conventional Diameter (8 mm) Grafts
Authors: Scorcia Vincenzo , Taloni Andrea , Rossi Costanza , Gioia Raffaella , Lucisano Andrea , Giannaccare Giuseppe 
Published: February 2023

Abstract
Objectives
Visual and topographic outcomes of large (9.0 mm) versus conventional (8.0 mm) deep anterior lamellar keratoplasty (DALK) for the treatment of keratoconus (KC) were compared in relation to the different localization of the corneal ectasia (within or beyond the central 8.0 mm).
Methods
This is a retrospective, comparative case series. Preoperatively, the topographic extension of the conus was calculated by measuring the distance from the geometric center of the cornea and the outermost point of the corneal ectasia (ectasia < 8.0 mm, group A; ectasia ≥ 8.0 mm, group B). DALK was performed using both small grafts (8.0 mm, group 1) and large grafts (9.0 mm, group 2). Best-corrected visual acuity and topographic astigmatism were evaluated preoperatively (T0) and postoperatively after complete suture removal (1 year, T1).
Results
Data from 224 eyes of 196 patients were evaluated. Topographic astigmatism improved in the entire series at T1 (4.94 ± 2.92 dioptres (D) [95% CI, 4.56–5.33] vs 4.19 ± 2.45 D [95% CI, 3.87–4.51], p = 0.001). There was no significant difference in postoperative topographic cylinder between group 1 and group 2 when considering eyes with corneal ectasia < 8.0 mm (group 1A, 4.15 ± 2.19 D [95% CI, 3.64–4.66] vs group 2A, 3.65 ± 2.13 D [95% CI, 2.92–4.38], p = 0.14); conversely, the difference was significant considering eyes with corneal ectasia ≥ 8.0 mm (group 1B, 4.74 ± 2.90 D [95% CI, 4.09–5.38] vs group 2B, 3.68 ± 1.94 D [95% CI, 3.10–4.26], p = 0.02).
Conclusions
Large 9.0-mm DALK provided better anatomical outcomes compared to conventional 8.0-mm DALK, particularly in eyes with corneal ectasia extending beyond the central 8.0 mm.
Effects of motor imagery based brain-computer interface on upper limb function and attention in stroke patients with hemiplegia: A randomized controlled trial
Authors: Liu Xiaolu , Zhang Wendong , Li Weibo , Zhang Shaohua , Lv Peiyuan , Yin Yu 
Published: February 2023

Abstract
Objective: To investigate the effects of motor imagery-based brain-computer interface training (MI-BCI) on upper limb function and attention in stroke patients with hemiplegia.
Methods: Stroke patients with impairment of upper extremity function and decreased attention were randomly assigned to the conventional rehabilitation group or the BCI group (1:1 ratio). BCI group performed 20 minutes of MI-BCI training five times a week for 3 weeks (15 sessions) in addition to conventional rehabilitation. The primary outcome measures were the changes in Fugl-Meyer Motor Function Assessment for Upper Extremities (FMA-UE) and Attention Network Test (ANT) from baseline to 3 weeks.
Results: A total of 60 people participated in the study, and about 93% of the patients completed the allocated training. Compared with the conventional rehabilitation group, among those in the BCI group, FMA-UE was increased by 8.0 points (95%CI, 5.0 to 10.0; P<0.001). Alert network response time (32.4ms; 95%CI, 58.4 to 85.6; P<0.001), orienting network response (5.6ms; 95%CI, 29.8 to 55.8; P=0.010), and corrects number (8.0; 95%CI, 17.0 to 28.0; P<0.001) also increased in the BCI group compared with the conventional rehabilitation group. Additionally, the executive control network response time (-105.9ms; 95%CI, -68.3 to -23.6; P=0.002), the total average response time (-244.8ms; 95%CI, -155.8 to -66.2; P=0.002), and total time (-122.0ms; 95%CI, -80.0 to -35.0; P=0.001) were reduced in the BCI group compared with the conventional rehabilitation group.
Conclusion: MI-BCI combined with conventional rehabilitation training could better enhance upper limb motor function and attention in stroke patients. This training method may be feasible and suitable for individuals with stroke.
Trial registration: This study was registered in the Chinese Clinical Trial Registry with Portal Number ChiCTR2100050430(27/08/2021).
Treatment of the Insensitive Munitions Compound, 3-Nitro-1,2,4-Triazol-5-one (NTO), in Flow Through Columns Packed with Zero-Valent Iron
Authors: Yu Youngjae , Root Robert A. , Sierra-Alvarez Reyes , Chorover Jon , Field Jim A. 
Published: February 2023

Abstract
The need for effective technologies to remediate the insensitive munitions compound 3-nitro-1,2,4-triazol-5-one (NTO) is emerging due to the increasing use by the U.S. Army and environmental concerns about the toxicity and aqueous mobility of NTO. Reductive treatment is essential for the complete degradation of NTO to environmentally safe products. The objective of this study is to investigate the feasibility of applying zero-valent iron (ZVI) in a continuous-flow packed bed reactor as an effective NTO remediation technology. The ZVI-packed columns treated an acidic influent (pH 3.0) or a circumneutral influent (pH 6.0) for six months (ca. 11000 pore volumes, PVs). Both columns effectively reduced NTO to the amine product, 3-amino-1,2,4-triazol-5-one (ATO). The column treating the pH-3.0 influent exhibited prolonged longevity in reducing NTO, treating 11-fold more PVs than the column treating pH-6.0 influent until the breakthrough point (defined as when only 85% of NTO was removed). The exhausted columns (defined as when only 10% of NTO was removed) regained the NTO reducing capacity by reactivation using 1 M HCl, fully removing NTO. After the experiment, solid phase analysis of the packed-bed material showed that ZVI was oxidized to iron (oxyhydr)oxide minerals such as magnetite, lepidocrocite, and goethite during NTO treatment. This is the first report on the reduction of NTO and the concomitant oxidation of ZVI in continuous-flow column experiments. The evidence indicates that treatment in a ZVI-packed bed reactor is an effective approach for the removal of NTO.
Assessing the suitability of lakes and reservoirs for recreation using Landsat 8 Water Clarity
Authors: Keith Darryl J. , Salls Wilson , Schaeffer Blake A. , Werdell P. Jeremy 
Published: February 2023

Abstract
Water clarity has long been used as a visual indicator of the condition of water quality. The clarity of waters is generally valued for aesthetic and recreational purposes. Water clarity is often assessed using a Secchi disk, attached to a measured line and lowered to a depth where it can be no longer seen. We have applied an approach which uses atmospherically corrected Landsat 8 data to estimate water clarity in freshwater bodies by using the Quasi-Analytical Algorithm (QAA) and Contrast Theory to predict Secchi depths for more than 270 lakes and ponds across the continental US. We found that incorporating Landsat 8 spectral data into methodologies created to retrieve the inherent optical properties (IOP) of coastal waters was effective at predicting in-situ measures of the clarity of inland lakes. The predicted Secchi depths were used to evaluate the recreational suitability for swimming and recreation using an assessment framework developed from public perception of water clarity. Results showed approximately 90 percent of the waterbodies in our dataset were classified as “marginally suitable to suitable” with less than 10 percent classed as “eminently suitable” to “totally unsuitable – unsuitable”. The implications are that satellites engineered for terrestrial applications can be successfully used with traditional ocean color algorithms and methods to measure the water quality of freshwater environments. Further, operational land-based satellite sensors have the temporal repeat cycles, spectral resolution, wavebands, and signal-to-noise ratios to be repurposed to monitor water quality for public use and trophic status of complex inland waters.
Psycho-social and health predictors of loneliness in older primary care patients and mediating mechanisms linking comorbidities and loneliness
Authors: Ljiljana Majnarić Trtica , Mile Volarić , Tomislav Kurevija , Silvio Mihaljević , Zdravka Krivdić , Wittlinger Thomas 
Published: February 2023

Abstract
Population aging is the global demographic trend in EU countries in the last decades. Aging is associated with unfavorable sociodemographic factors such as widowhood, lower physical and mental functioning, and fewer possibilities for social participation. All these factors have been found to be associated with loneliness – a feeling of social isolation. Living with feeling of loneliness may, in turn, have negative implications on quality of life, physical and mental health, and mortality, of elderly individuals. The aim was to assess associations of feeling of loneliness with sociodemographic and psycho-social characteristics and comorbidities of older individuals from the general population. The sample consisted of 189 (58% F) older individuals (> 50 years) (mean ± SD, 78.47 ± 6.65), attenders in Primary Health Care. Apart from sociodemographic characteristics and comorbidities, participants were described by psychological characteristics, assessed by a set of standard questionnaires, including: UCLA loneliness scale, MSPSS (Multidimensional Scale of Perceived Social Support), GAS (Geriatric Anxiety Scale), GDS (Geriatric Depression Scale), ADL (Activities of Daily Living), IADL (Instrumental Activities of Daily Living), GSE (General Self-Efficacy) and ERQ (Emotional Regulation Questionnaire). The effect of particular blocks of factors on status loneliness was assessed by the hierarchical regression model. We also analyzed if any of psychological characteristics mediate associations between comorbidities and loneliness, when controlling for sociodemographic characteristics. The results indicated that older individuals who lived alone, had difficulties in relations with their family members, suffered from depression, and had lower level of education, felt much lonely. Individuals who were generally engaged in hobbies, were less lonely. The level of comorbidity was shown to be associated with loneliness, with some diagnoses contributing more than some others. Some psychological traits can lessen feeling of loneliness, while anxiety and depression make it worsen. Mediation analysis informed us on how to help patients with comorbidities to feel less lonely, including interventions such as those that can reduce anxiety and depression, improve cognitive abilities and emotional regulation, and enhance social support.
Neutral lipid storage disease with myopathy and myotonia associated to pathogenic variants on PNPLA2 and CLCN1 genes: case report.
Authors: Landim João Igor Dantas , Ribeiro Ian Silva , de Oliveira Eduardo Braga , Freitas Hermany Capistrano , de Brito Lara Albuquerque , Maia Isaac Holanda Mendes , Távora Daniel Gurgel Fernandes , Rodrigues Cleonisio Leite 
Published: February 2023

Abstract
Background:
Neutral lipid storage disease with myopathy (NLSD-M) is an autosomal recessive disease that manifests itself around the 3rd to 4th decade with chronic myopathy of proximal predominance in the shoulder girdle. Clinical myotonia is uncommon. We will report a rare case associated with pathogenic mutations on PNPLA2 and CLCN1 genes with a mixed phenotype of NLSD-M and a subclinical form of Thomsen’s congenital myotonia.
Case presentation:
We describe a patient with chronic proximal myopathy, very subtle clinical myotonia and electrical myotonia on electromyography (EMG). Serum laboratory analysis disclosure hyperCKemia (CK 1280 mg/dL). A blood smear analysis showed Jordan’s anomaly, a hallmark of NLSD-M. A genetic panel was collected using the NGS technique, which identified two pathogenic variants on genes supporting two different diagnosis: NLSD-M and Thomsen congenital myotonia, whose association has not been previously described.
Conclusions:
Although uncommon, it is important to remember the possibility of association of pathogenic mutations to explain a specific neuromuscular disease phenotype. The use of a range complementary methods, including myopathy genetic panels, may be essential to diagnostic definition in such cases.
Does quadriceps strength asymmetry is able to predict functional decline, gait abnormalities and falls in community-dwelling older adults? A cohort study
Authors: MARQUES NISE RIBEIRO , Brando Natalia Dias , Santos Giovanny Viegas dos 
Published: February 2023

Abstract
Introduction.
Aging and articular degenerative inflammatory diseases, such as osteoarthritis, decline quadriceps strength. The reduction on quadriceps strength usually is asymmetric, and it may leads to an inefficient pattern to absolve ground reaction forces on the weaker limb during walking.
Objective.
The present study aimed to identify the ability quadriceps strength asymmetry to predict gait abnormalities, functional decline and falls prevalence in community-dwelling older adults.
Methods.
Participated of this study 52 older adults who live on a community-dwelling setting. The participants were separated into two groups according having or not knee extensor muscles strength asymmetry. Strength asymmetry was considered when the difference of strength between right and left maximal quadriceps maximal strength is higher than 20%. Twenty-four participants composed the strength asymmetry group and twenty-eight participants composed the control group. For the data collection: participants answered a characterization form; performed Short Physical Performance Battery and 400-m walking tests; three maximal isometric voluntary knee extensors contraction of right and left legs were recorded; and gait kinematics data was collected. The follow-up of the study was conducted after six months. A multivariate analysis of variance was used to compare the parameters, stepwise test was used to identify the predictive parameters and a ROC curve was used to determine the sensitive and specificity. The significant level was set at p < 0.05.
Results.
The quadriceps strength asymmetry was 28.9% in the group with strength asymmetry and the control group have 15% of quadriceps asymmetry. A strength asymmetry of 25% was able to predict functional a decline on the 400-m walking test (sensitivity = 78% and specificity = 82%); falls prevalence (sensitivity = 76,9% and specificity = 96,1%); and gait abnormalities, such as increased stance and stride time variability (sensitivity = 68% and specificity = 80%; and sensitivity = 71% and specificity = 84%, respectively).
Conclusion.
Quadriceps strength asymmetry higher than 25% may predict falls prevalence in community-dwelling older adults. Also, the quadriceps strength asymmetry predicts a functional decline, and temporal and spatial gait abnormalities those could reduce gait performance, promote early fatigue, and increase the risk of falls in older adults.
Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics
Authors: Zhang Yu , Shi Chuanbing , Yang Yiqiong , Huang Jing , Hu Xiuxiu , Ni Haifeng , Li Li , Cheng Zhengyuan , Chen Pingsheng 
Published: February 2023

Abstract
Renal interstitial fibrosis (RIF) is the prominent pathological characteristics of deteriorative chronic kidney disease (CKD), leading to peritubular capillary (PTC) rarefaction accompanied by obvious hypoxia. However, the underlying mechanisms remain elusive. To deal with this, we constructed a comparative transcriptome analysis of hypoxia and normoxia induced HK-2 cells model to discover total 572 differentially expressed genes (DEGs), which were involved in extensive biological processes. Moreover, these DEGs were uncovered to regulate RIF mainly through HIF-1 signaling pathway from KEGG enrichment. Hence, chip sequencing of HK-2 hypoxia was used to identify 2915 favorable genes modulated by HIF-1α through peak annotation. To translate bioinformatic data into credible clinical application, 43 intersections were then found via a combination of RNA sequencing and chip sequencing. In addition to angiogenesis of GO analysis, 2 candidates including VEGFA and BTG1 were finally recommended as novel targets by annotating their binding sites, which significantly interacted with HIF-1α. Our study provided ascendant insights into the molecular mechanism’s alterations of RIF, therefore paving the intervention therapeutics.
Digital advances in health education with fun health games for children
Authors: Efe Hacer , Topsakal Ünsal Umdu 
Published: February 2023

Abstract
Health education is a life-long process for every individual and improves the health and quality of life of the society and it is important to benefit from interactive digital learning environments in providing health education for the digital children of the new age. The aim of the study is to develop educational digital health games and to investigate the role of primary school students in health awareness. Accordingly, in this study, various educational digital health games were designed, developed and evaluated in many health areas, healthy daily life, dental health, hygiene/cleaning, diseases and coronavirus disease. The study is a design-based research and the ASSURE model, one of the design-based research types, was used in the study. The sample of the study consists of 12 primary school 4th grade students. In the study, semi-structured interview was used as data collection tool and content analysis was applied in the analysis. As a result of the study, it was found that educational digital health games improved the health awareness of primary school students and the students gave more and various correct answers after the implementation than before the implementation. Accordingly, educational digital health games can be used in health education for children.
Inhalable CAR-T Cell-Derived Exosomes as Paclitaxel Carriers for Treating Lung Cancer
Authors: Zheng Wei , Zhu Tianchuan , Tang Lantian , Li Zhijian , Jiang Guanmin , Huang Xi 
Published: February 2023

Abstract
Background
Non-small cell lung cancer (NSCLC) is a worldwide health threat with high annual morbidity and mortality. Chemotherapeutic drugs such as paclitaxel (PTX) have been widely applied clinically. However, systemic toxicity due to the non-specific circulation of PTX often leads to multi-organ damage, including to the liver and kidney. Thus, it is necessary to develop a novel strategy to enhance the targeted antitumor effects of PTX.
Methods
Here, we engineered exosomes derived from T cells expressing the chimeric antigen receptor (CAR-Exos), which targeted mesothelin (MSLN)-expressing Lewis lung cancer (MSLN-LLC) through the anti-MSLN single-chain variable fragment (scFv) of CAR-Exos. PTX was encapsulated into CAR-Exos (PTX@CAR-Exos) and administered via inhalation to an orthotopic lung cancer mouse model.
Results
Inhaled PTX@CAR-Exos accumulated within the tumor area, reduced tumor size, and prolonged survival with little toxicity. In addition, PTX@CAR-Exos reprogrammed the tumor microenvironment and reversed the immunosuppression, which was attributed to infiltrating CD8+ T cells and elevated IFN-γ and TNF-α levels.
Conclusions
Our study provides a nanovesicle-based delivery platform to promote the efficacy of chemotherapeutic drugs with fewer side effects. This novel strategy may ameliorate the present obstacles to the clinical treatment of lung cancer.
Fetal Testicular Teratoma: Report of a Prenatally Undiagnosed Case and Literature Review
Authors: Yue Qingxiong , Sha Yujia , Liu Yan 
Published: February 2023

Abstract
Background: Neonatal testicular teratoma is very rare. In addition, testicular teratoma diagnoses are usually missed during the relatively short neonatal period. We report the case of an infant with an undiagnosed testicular teratoma, who presented clinical signs during the prenatal and neonatal periods. We also reviewed the literature for ultrasonographic findings of prenatal and infancy testicular teratomas in an attempt to promote timely diagnoses and treatments for testicular teratomas.
Case presentation: A 29-year-old pregnant woman underwent a routine obstetric examination at 30w+2d of gestation. Doppler ultrasound examination revealed a cystic mass (30 × 26 × 26 mm) in the left lower abdomen of the fetus, next to the bladder. The inner wall of the cyst enveloped a heterogeneous hyperechoic solid mass of approximately 7 × 7 mm in size, without a blood flow signal. Regular ultrasound monitoring was continued during the pregnancy and neonatal periods. The infant underwent left testicular tumor resection at 10 months and 11 days after birth. The postoperative pathological result revealed a mature teratoma; after the treatment, the child presented no recurrences at the six-month follow-up visit.
Conclusions: Testicular teratomas should be suspected in fetuses with abdominal mixed cyst-solid or solid masses (especially in the presence of solid component calcifications) and an empty scrotum.
Contribution of boundary non-stoichiometry to the lower-temperature plasticity in high-pressure sintered boron carbide
Authors: Xu Haiyue , Ji Wei , Jiang Jiawei , Liu Junliang , Yu Ruohan , Tu Bingtian , Zhang Jinyong , Zou Ji , Wang Weimin , Wu Jinsong , Fu Zhengyi 
Published: February 2023

Abstract
Non-oxide ceramics exhibit significant advantages in high-tech industry, but their applications have been limited by the brittle nature especially at low to moderate temperatures. It is a great challenge to improve the ceramic plasticity while maintaining the high-temperature strength through the classical strategy, which generally includes decreasing grain size to several nanometers or adding ductile binder phase. Herein, the plasticity of fully dense boron carbide (B4C) has been greatly enhanced due to the boundary non-stoichiometry induced by high-pressure sintering technology. The effect decreased the plastic deformation temperature of B4C by 200°C compared to that of conventionally-sintered specimens. Promoted grain boundary diffusion is found to enhance grain boundary sliding, which dominate the lower-temperature plasticity. In addition, the as-produced specimen maintained extraordinary strength before the occurrence of plasticity. The study provides a new and efficient strategy by boundary chemical change to facilitate the plasticity of ceramic materials.