bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bayesian modelling of response to therapy and drug-sensitivity in acute lymphoblastic leukemia
Authors: Cremaschi Andrea , Yang Wenjian , De Iorio Maria , Evans William , Yang Jun , Rosner Gary 
Published: February 2023

Abstract
Acute lymphoblastic leukemia (ALL) is a heterogeneous haematologic malignancy involving the abnormal proliferation of immature lymphocytes and accounts for most paediatric cancer cases. The management of ALL in children has seen great improvement in the last decades thanks to greater understanding of the disease leading to improved treatment strategies evidenced through clinical trials. Common therapy regimens involve a first course of chemotherapy (induction phase), followed by treatment with a combination of anti-leukemia drugs. A measure of the efficacy early in the course of therapy is the presence of minimal residual disease (MRD). MRD quantifies residual tumor cells and indicates the effectiveness of the treatment over the course of therapy. MRD positivity is defined for values of MRD greater than 0.01%, yielding left-censored MRD observations. We propose a Bayesian model to study the relationship between patient features (leukemia subtype, baseline characteristics, and drug sensitivity profile) and MRD observed at two time points during the induction phase. Specifically, we model the observed MRD values via an auto-regressive model, accounting for left-censoring of the data and for the fact that some patients are already in remission after the first stage of induction therapy. Patient characteristics are included in the model via linear regression terms. In particular, patient-specific drug sensitivity based on ex vivo assays of patient samples is exploited to identify groups of subjects with similar profiles. We include this information as a covariate in the model for MRD. We adopt horseshoe priors for the regression coefficients to perform variable selection to identify important covariates. We fit the proposed approach to data from three prospective paediatric ALL clinical trials carried out at the St. Jude Children’s Research Hospital. Our results highlight that drug sensitivity profiles and leukemic subtypes play an important role in the response to induction therapy as measured by serial MRD measures.
Is MCC950, an NLRP3 inflammasome inhibitor, a renopreventive: A chronic renal failure adenine model in rats
Authors: Sabra Mahmoud S. , Hemida Fahmy K. , Allam Essmat A.H. 
Published: February 2023

Abstract
Background: Chronic renal failure (CRF) is characterized by a great loss in renal function ending with reduced sodium filtration and suppression of tubular reabsorption that eventually leads to volume expansion. The contradictory information on the MCC950 renal effects brought this study to evaluate the possible renopreventive effects of NLRP3 inflammasome inhibitor MCC950 in adenine-induced CRF in rats.
Methods: Adding 0.75% of adenine powder to a rat’s ration daily for 4 weeks is measured as a broad approval to be a model of evaluation of kidney damage as it is similar to most of the kidney tubular alterations seen in chronic kidney-diseased patients. Blood pressure was monitored at baseline and weekly during the test. Also, oxidative stress factors, urine sample analysis, histopathological changes, and immunohistochemical alterations of caspase-3 and interleukin-1 beta (IL-1β) levels in renal tissues were done.
Results: Results showed that the NLRP3 inflammasome inhibitor MCC950 had a renopreventive effect that was established by a decrease in blood pressure measurements, and improvement of urinary, serum, and renal tissue markers as revealing organ damage. This was also revealed by the reduction of tubular expression of neutrophil gelatinase-associated lipocalin (NGAL).
Immunohistochemical studies revealed that the worsened renal cellular changes indicated by raised expression of caspase-3 and IL-1β were prominently improved by NLRP3 inflammasome inhibitor MCC950 administration.
Conclusion: The NLRP3 inflammasome inhibitor MCC950 administration in the CRF rat model had renopreventive effects which may be a potential therapeutic approach to prevent the progression of CRF.
Starch-dependent sodium accumulation in the leaves of Vigna riukiuensis.
Authors: Noda Yusaku , Hirose Atsushi , Wakazaki Mayumi , Sato Mayuko , Toyooka Kiminori , Kawachi Naoki , Furukawa Jun , Tanoi Keitaro , Naito Ken 
Published: February 2023

Abstract
This research provides insight into a unique salt tolerance mechanism of Vigna riukiuensis. V. riukiuensis is one of the great genetic resources of salt tolerance. We have previously reported that V. riukiuensis accumulates a higher amount of sodium in the leaves, whereas V. nakashimae, a close relative of V. riukiuensis, suppresses sodium allocation to the leaves. We first suspected that V. riukiuensis would have developed vacuoles for sodium sequestration, but there were no differences compared to a salt-sensitive accession. However, many starch granules were observed in the chloroplasts of V. riukiuensis. In addition, forced degradation of leaf starch by shading treatment resulted in no radio-Na (22Na) accumulation in the leaves. We performed SEM-EDX to locate Na in leaf sections and detected Na in chloroplasts of V. riukiuensis, especially around the starch granules but not in the middle of those. Our results could provide the second evidence of Na-trapping system by starch granules, following the case of common reed that accumulates starch granule at the shoot base for binding Na.
Familial dysalbuminemic hyperthyroxinemia combined with Graves’ disease: A case report and literature review
Authors: Li Yuanmeng , Chi Yue , Chai Xiaofeng , Liu He , Li Naishi , Lian Xiaolan 
Published: February 2023

Abstract
Background: Familial dysalbuminemic hyperthyroxinemia (FDH) is a dominantly inherited disease characterized by abnormally increased affinity of albumin for serum thyroxine. Assay interference and differential diagnosis remain challenges for FDH. The condition becomes more complicated when FDH combined with primary thyroid diseases. The co-occurrence of FDH and Graves’ disease is one of the rare cases.
Case presentation: We reported a 28-year-old woman with a complex condition of FDH coexisted with Graves’ disease. The existence of FDH was not recognized at first. And the Graves’ disease was relieved after treatment with antithyroid drugs and two times of radioactive iodine therapy. Then she developed to primary hypothyroidism and received levothyroxine replacement. But the thyroid function failed to reach normal despite of frequent adjustment of levothyroxine. Finally, syndromes of inappropriate secretion of thyroid stimulating hormone (IST) were considered and FDH was successfully distinguished from other causes of IST.
Conclusions: More attention should be paid to FDH when investigating the causes of IST. Early recognition of FDH is critical to rightly evaluate the thyroid function status and avoid inappropriate treatment, especially in complicated cases with concurrent FDH and primary thyroid diseases.
Prenatal Genetic Analysis of Kidney Abnormalities
Authors: Shen Yongmei , Li Yaqi , Zhao Xiaomin , Li Wen , Zhang Lei , Yao Liying , Cao Jiasong , Li Shanshan , Wei Zhuo , Chang Ying 
Published: February 2023

Abstract
Objective: To systematically analyze the genetic features of fetal renal abnormalities and the prenatal characteristics of 17q12 microdeletion syndrome.
Methods: We retrospective analysis of fetal diagnosed with renal abnormalities from January 2016 to August 2022. Chromosome test, fetal renal abnormalities and pregnancy outcomes were performed in a descriptive analysis.
Results: 141 patients (4.5%) showed abnormal renal development and 26 patients (26/141) with hyperechogenic kidneys (HCK), 14 (14/26) cases showed chromosomal abnormalities, 12 patients were 17q12 microdeletion syndrome. In addition, there were 14 17q12 microdeletion syndrome among the patients, and 12 fetal had hyperechogenic kidneys (HCK), 7 fetal were verified by couples, 5 fetal had new mutations, and 2 fetal inherited from parents. After excluding patients with HCK, the polycystic kidney has a higher rate of chromosomal abnormalities compared to polycystic renal dysplasia and renal dysplasia. The chromosomal of isolated horseshoe kidney, hydronephrosis, ectopic kidney and unilateral kidney often were normal, and the chromosomal abnormalities rate was increased when combined with other abnormalities.
Conclusion: Fatal HCK was closely related to 17q12 microdeletion syndrome. The chromosome test result of patients with isolated polycystic kidney dysplasia, kidney dysplasia, horseshoe kidney, hydronephrosis, kidney deficiency, and ectopic kidney were mostly normal. Prenatal diagnosis was recommended, specifically in the fetus had non-isolated renal abnormalities. Most patients with normal chromosomes or uncertain clinical significance choose to produce, but adverse pregnancy outcomes were not excluded. This study will provide more data support for fetal renal abnormalities.
Synergic quantum generative machine learning
Authors: Bartkiewicz Karol , Tulewicz Patrycja , Roik Jan , Lemr Karel 
Published: February 2023

Abstract
We introduce a new approach towards generative quantum machine learning significantly reducing the number of hyperparametersand report on a proof-of-principle experiment demonstrating our approach. Our proposal depends on collaboration betweenthe generators and discriminator, thus, we call it quantum synergic generative learning. We present numerical evidence thatthe synergic approach, in some cases, compares favorably to recently proposed quantum generative adversarial learning.In addition to the results obtained with quantum simulators, we also present experimental results obtained with an actualprogrammable quantum computer. We investigate how a quantum computer implementing generative learning algorithm couldlearn the concept of a maximally- entangled state. After completing the learning process, the network is able both to recognizeand to generate an entangled state. Our approach can be treated as one possible preliminary step to understanding how theconcept of quantum entanglement can be learned and demonstrated by a quantum computer.
Gastrointestinal parasites of zoonotic importance detected in porcine faeces in Chitwan National Park, Nepal
Authors: Subedi Janak Raj , Neupane Sanskar , Dhakal Pitambar 
Published: February 2023

Abstract
Wild boar (Sus scrofa) is considered to be a potential source of zoonotic parasites. Wild boars are found at considerable number in and around the Chitwan National Park (CNP). The information regarding their intestinal parasites is limited. A cross-sectional study was carried out to determine the prevalence of gastrointestinal parasites in wild boars in CNP. A total of 100 fresh fecal samples were subjected to microscopic examination using direct smear, floatation and sedimentation method. Overall, 95% fecal samples were found positive for at least one parasite. Prevalence of protozoan parasites was found comparatively higher (70%) followed by nematode (56%) and trematode (12%). Nine gastrointestinal parasites Eimeriasp. (70% without micropyle and 40% with micopyle), Fasciola sp. (12%), Strongyloides sp. (56%), Strongyle type (49%), Stephanurus sp. (44%), Globocephalus sp. (38%), Metastrongylus sp. (12%), Ascaris sp. (7%) and Trichuris sp. (6%).) were recorded. Eimeria sp. exhibited the highest prevalence while Trichuris had the least prevalence. This study has provided baseline information regarding the diversity of gastrointestinal parasite in wild boars. It requires continuous study at molecular level to explore other species of parasites and verify their zoonotic potential.
Informational active matter
Authors: VanSaders Bryan , Vitelli Vincenzo 
Published: February 2023

Abstract
Many biomolecular systems can be viewed as ratchets that rectify environmental noise through measurements and information processing. As miniaturized robots cross the scale of unicellular organisms, on-board sensing and feedback open new possibilities for propulsion strategies that exploit fluctuations rather than fight them. Here, we study extended media in which many constituents display a feedback control loop between measurement of their microstates and the capability to bias their noise-induced transitions. We dub such many body systems informational active matter and show how information theoretic arguments and kinetic theory derivations yield their macroscopic properties starting from microscopic agent strategies. These include the ability to self-propel without applying work and to print patterns whose resolution improves as noise increases. We support our analytical results with extensive simulations of a fluid of `thinker' type particles that can selectively change their diameters to bias scattering transitions. This minimal model can be regarded as a non-equilibrium analogue of entropic elasticity that exemplifies the key property of this class of systems: self-propulsive forces grow ever stronger as environmental noise increases thanks to measurements and control actions undertaken by the microscopic constituents. We envision applications of our ideas ranging from noise induced patterning performed by collections of microrobots to reinforcement learning aided identification of migration strategies for collections of organisms that exploit turbulent flows or fluctuating chemotactic fields.
Analysis and Assessment of Ground Motion Characteristics and Similarity Using Dynamic Time Warping Distance
Authors: He Haoxiang , Sun Haoding , Chen Yifei 
Published: February 2023

Abstract
Due to the complex nonstationarity of ground motion in time-frequency domain, the traditional methods of comparing and evaluating earthquake waveforms have not enough ability and accuracy to distinguish the details and changing features of the similar waves, which makes the similarity evaluation of waveform is difficult to be quantified accurately. The similarity degree of different signals can be calculated precisely according to Dynamic time warping (DTW) algorithm, so it can be used for waveform comparison and similarity evaluation. In order to improve the traditional method, a method based on DTW distance is proposed to identify the earthquake waveform and analyze the ground motion characteristics. Based on the statistical analysis of a great quantity of earthquake waves, the changes law of DTW distance considering amplitude, time lag, noise signal ratio, site type and the comprehensive effect is obtained. DTW distance is proved to be used as a compatible evaluation standard for waveform refinement. It is verified that DTW distance and vector norm are essentially equivalent. In the analysis of ground motion, DTW distance is implicated in the equivalent amplitude and energy of earthquake waves. The physical connotation of DTW distance is demonstrated by analyzing the data of the station array, and the results show that the distribution of DTW distance can accurately imply the time-space variation effect of the earthquake in the region. The reasonable reference range of DTW distance is defined by statistical method, and the corresponding evaluation standard of synthetic multi-point ground motion with real characteristics is proposed. In the synthetic accuracy evaluation of artificial ground motion with spatial variation effect, the combination of ground motions with more real characteristics can be obtained by evaluating and optimizing the waveforms according to the variation rule and range of DTW distance.
Fear of cancer Recurrence Despite a good prognosis: A Qualitative study with Thyroid Cancer Patients
Authors: Lu Geling , Luo Weixiang , Lin Junqing , Xu Nan , Zheng Chunrao 
Published: February 2023

Abstract
Objective: Thyroid cancer’s emotional and mental toll is often beyond the disease’s duration. The fear of cancer recurrence (FCR) was prominent among cancer patients and survivors, but there are few studies on this population. This study aims to explore the experience of FCR and expand understanding of FCR in patients with thyroid cancer.
Methods: In this qualitative study, 18 patients with thyroid cancer were included via semi-structured interview. The average age of the respondents is 41-year-old, and the average time since diagnosis is 2 years.
Results: In the process of exploring the experience of FCR in thyroid cancer patients, the following three themes have emerged: First, "uncertainty of disease" refers to the uncertainty in face of cancer. Most of the participants are committed to trying to return to the state before diagnosis. The second is the "triggers for FCR", which involves internal and external factors. The third is the diversification of FCR management, which included fatalism and active response to seek help.
Conclusions: The fear of cancer recurrence in thyroid cancer patients needs further understanding. Medical staff can reduce or buffer FCR in thyroid cancer patients by treating triggers and strengthening positive changes.
Sodium hydrogen sulfide alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via the inhibition of the ubiquitination and degradation of IKB-α
Authors: Fei Wenchao , Tong Wenqin , Xu Ke , Huo Ziqi , Hong Yang , Li Yinghua 
Published: February 2023

Abstract
Background: Although several effective therapies are available for the treatment of postmenopausal osteoporosis (PMO), the most common type of primary osteoporosis (OP). More effective and acceptable drugs to cure postmenopausal osteoporosis were needed. NaHS, the donor of H2S, may be one of the drugs to treat PMO, but its role and mechanism are still unclear.
Methods：Ovariectomized mice and Sham operation mice, BMMs and RAW264.7 cell lines were used to illustrate the in vivo and in vitro effects of NaHS on the osteoclast differentiation. On the other hand, molecular and histological methods were applied to evaluate the osteoclast differentiation and investigate the in vivo and in vitro mechanism.
Results: Phenotypically, NaHS treatment can increase the bone mineral density and bone quality of osteoporosis models induced by ovariectomy (OVX) in mice. Mechanistically, NaHS inhibited the nuclear translocation of p65 by inhibiting the ubiquitination and proteasome degradation of IkB-α.
Conclusions: NaHS protects against OVX-induced bone loss by inhibiting osteoclastic bone resorption. It plays an important role in inhibiting osteoclast differentiation and protecting against bone loss in PMO and it is potential for preventing and treating PMO.
Synergistic effects of miR-143 with miR-99a inhibited cell proliferation and induced apoptosis in breast cancer
Authors: Doosti Zahra , Ebrahimi Syed Omar , Ghahfarokhi Mahshid Samie , Reiisi Somayeh 
Published: February 2023

Abstract

Introduction: Breast cancer (BC) is the most common cancer type and the fifth leading cause of cancer-related death. The primary goals of BC treatment are to remove the tumor and prevent metastasis. Despite advances in BC treatment, more effective therapies are required. miRNAs can regulate many targets involved in biological processes and tumor progression; these molecules have emerged as a promising cancer treatment strategy. In the present study, we investigated the effects of miR-99a and miR-143 in based single expression plasmids for BC inhibition.
Methods: In this study, the precursor structure of miRNAs in the expression vector pEGFP-N1 entered single and double states, and MCF7 and T47D cells were transfected. The miRNAs expression level after transfection was then measured using qPCR. The MultiMiR package was used to obtain predicted and validated miRNA targets. MTT assay, qRT-PCR, migration test, and flow cytometry were used to assess the effect of miRNA and gene modulation.
Results: The qPCR results revealed that miRNA constructs were significantly expressed after the transfection of both cell lines. The biological function of miRNAs showed that upregulation of miR-99a and miR-143 in any of the two selected BC cells inhibited their proliferation and migration rate, significantly inducing apoptosis (p<0.01). Also, miR-99a/-143 co-treatment has a synergistic anticancer effect in cancer cells via Akt1 and CDK6 targeting.
Conclusion: These findings suggest that miR-99a/-143 plays synergistic regulatory roles in BC, possibly via a shared signaling pathway, providing a therapeutic strategy for BC treatment.
Efflux pump in genomic island GI-M202a mediates transfer of Polymyxin B resistance in Pandoraea pnomenusa M202
Authors: Gao Wenhui , Li Congcong , Wang Fengtian , Wang Zhongxue , Chen Xi , Tan Meixia , Fu Jiafang , Zhang Peipei , Cao Guangxiang , Zong Gongli 
Published: February 2023

Abstract
Background
Polymyxin B was thought to be last-line therapeutic options against multidrug-resistant Gram-negative bacteria, especially in COVID-19 co-infections or other serious infections. The risk of antimicrobial resistance and its spread to the environment should be brought to the forefront.
Methods
P. pnomenusa M202 was isolated under selection with 8 mg/L polymyxin B from hospital sewage. The genome of M202 was sequenced by PacBio RS II and Illumina HiSeq 4000 platforms. MFS transporter recombinant E. coli strain Mrc-3 was constructed by transferring encoding gene FKQ53_RS21695, a gene in Genomic islands (GIs) of M202, to E. coli 25DN. Mating experiments were performed to evaluate transfer of MFS transporter to Escherichia coli 25DN. And influences of efflux pump inhibitors on MICs were determined. The mechanism of polymyxin B excretion mediated by FKQ53_RS21695 was investigated by Discovery Studio 2.0 based on its homologous model.
Results
MICs of multidrug-resistant bacterial strain P. pnomenusa M202, isolated from hospital sewage, for polymyxin B is 96 mg/L. Genomic Island GI-M202a with major facilitator superfamily (MFS) transporter encoding gene and conjugative transfer proteins encoding genes of type IV secretion system was identified in strain M202. The mating experiment between M202 and Escherichia coli 25DN reflected the transferability of polymyxin B resistant GI-M202a. Efflux pump inhibitor and heterogeneous expression assays also suggested that MFS transporter gene FKQ53_RS21695 in GI-M202a was responsible to polymyxin B resistance. Molecular docking revealed that polymyxin B fatty acyl group insert into hydrophobic region of transmembrane core with Pi-alkyl and unfavorable bump interactions, and then polymyxin B turns over around Tyr43 to left the peptide group to outside during efflux process, accompanies with conformation change of MFS transporter from inward to outward. Additionally, verapamil and CCCP demonstrated significant inhibition by competing binding sites.
Conclusions
These findings demonstrated that GI-M202a along with MFS transporter FKQ53_RS21695 in P. pnomenusa M202 could mediate the transmission of polymyxin B resistances.