bluuebunny/crossref_metadata_2025_split huggingface dataset:
Technical failure rates for biometry between swept-source and older-generation optical coherence methods: a review and meta-analysis
Authors: Kanclerz Piotr , Hecht Idan , Tuuminen Raimo 
Published: April 2023

Abstract
Purpose
Precise ocular measurements are fundamental for achieving excellent target refraction following both cataract surgery and refractive lens exchange. Biometry devices with swept-source optical coherence tomography (SS-OCT) employ longer wavelengths (1055–1300 nm) in order to have better penetration through opaque lenses than those with partial coherence interferometry (PCI) or low-coherence optical reflectometry (LCOR) methods. However, to date a pooled analysis showing the technical failure rate (TFR) between the methods has not been published. The aim of this study was to compare the TFR in SS-OCT and in PCI/LCOR biometry.

Methods
PubMed and Scopus were used to search the medical literature as of Feb 1, 2022. The following keywords were used in various combinations: optical biometry, partial coherence interferometry, low-coherence optical reflectometry, swept-source optical coherence tomography. Only clinical studies referring to patients undergoing routine cataract surgery, and employing at least two (PCI or LCOR vs. SS-OCT) optical methods for optical biometry in the same cohort of patients were included.

Results
Fourteen studies were included in the final analysis, which presented results of 2,459 eyes of at least 1,853 patients. The overall TFR of all included studies was 5.47% (95% confidence interval [CI]: 3.66–8.08%; overall I2 = 91.49%). The TFR was significantly different among the three methods (p < 0.001): 15.72% for PCI (95% CI: 10.73–22.46%; I2 = 99.62%), 6.88% for LCOR (95% CI: 3.26–13.92%; I2 = 86.44%), and 1.51% for SS-OCT (95% CI: 0.94–2.41%; I2 = 24.64%). The pooled TFR for infrared methods (PCI and LCOR) was 11.12% (95% CI: 8.45–14.52%; I2 = 78.28%), and was also significantly different to that of SS-OCT: 1.51% (95% CI: 0.94–2.41%; I2 = 24.64%; p < 0.001).

Conclusions
A meta-analysis of the TFR of different biometry methods highlighted that SS-OCT biometry resulted in significantly decreased TFR compared to PCI/LCOR devices.
Dry needling in a multimodal rehabilitation protocol following rotator cuff repair surgery: study protocol for a double-blinded randomized sham-controlled trial
Authors: Naseri Faeze , Dadgoo Mehdi , Pourahmadi Mohammadreza , Amroodi Morteza Nakhaei , Azizi Shirin , Tabrizian Pouria , Amiri Ali 
Published: April 2023

Abstract
Background
Rotator cuff tear (RCT) is one of the main causes of shoulder pain and dysfunction. Rotator cuff repair (RCR) is a common surgical procedure for the management of RCTs. Presence of myofascial trigger points (MTrP) as a result of surgical procedure can aggravate postoperative shoulder pain. The purpose of this protocol is to describe a randomized controlled trial design to evaluate the effect of implementing 4 sessions of myofascial trigger point dry needling (MTrP-DN) within a multimodal rehabilitation protocol following RCR surgery.

Methods
Forty-six participants aged 40–75 will be recruited having postoperative shoulder pain after RCR and meeting the inclusion criteria. Participants will be randomly divided into 2 groups: One group will undergo MTrP-DN, manual therapy, exercise therapy and electrotherapy and the other will receive sham dry needling (S-DN), manual therapy, exercise therapy and electrotherapy. This protocol will cover 4 weeks of intervention. The primary outcome measure will be the Numeric Pain Rating Scale (NPRS) for pain. Secondary outcome measures will be Shoulder Pain and Disability Index (SPDI), range of motion (ROM), strength and adverse events.

Discussion
This is the first study to investigate the use of 4 sessions of MTrP-DN in combination with a multimodal rehabilitation protocol for postoperative shoulder pain, restriction, weakness and dysfunction following RCR. The results of this study may help to determine the effect of MTrP-DN on various outcomes after RCR surgery.

Trial registration
This trial was registered at the (https://www.irct.ir), (IRCT20211005052677N1) on 19/2/2022.
TRPM4 regulates hilar mossy cell loss in temporal lobe epilepsy
Authors: Mundrucz Laura , Kecskés Angéla , Henn-Mike Nóra , Kóbor Péter , Buzás Péter , Vennekens Rudi , Kecskés Miklós 
Published: April 2023

Abstract
Background
Mossy cells comprise a large fraction of excitatory neurons in the hippocampal dentate gyrus, and their loss is one of the major hallmarks of temporal lobe epilepsy (TLE). The vulnerability of mossy cells in TLE is well known in animal models as well as in patients; however, the mechanisms leading to cellular death is unclear.

Results
Transient receptor potential melastatin 4 (TRPM4) is a Ca2+-activated non-selective cation channel regulating diverse physiological functions of excitable cells. Here, we identified that TRPM4 is present in hilar mossy cells and regulates their intrinsic electrophysiological properties including spontaneous activity and action potential dynamics. Furthermore, we showed that TRPM4 contributes to mossy cells death following status epilepticus and therefore modulates seizure susceptibility and epilepsy-related memory deficits.

Conclusions
Our results provide evidence for the role of TRPM4 in MC excitability both in physiological and pathological conditions.
Genetic evidence implicating natriuretic peptide receptor-3 in cardiovascular disease risk: a Mendelian randomization study
Authors: Cronjé Héléne T. , Karhunen Ville , Hovingh G. Kees , Coppieters Ken , Lagerstedt Jens O. , Nyberg Michael , Gill Dipender 
Published: April 2023

Abstract

Background
C-type natriuretic peptide (CNP) is a known target for promoting growth and has been implicated as a therapeutic opportunity for the prevention and treatment of cardiovascular disease (CVD). This study aimed to explore the effect of CNP on CVD risk using the Mendelian randomization (MR) framework.

Methods
Instrumental variables mimicking the effects of pharmacological intervention on CNP were identified as uncorrelated genetic variants located in the genes coding for its primary receptors, natriuretic peptide receptors-2 and 3 (NPR2 and NPR3), that associated with height. We performed MR and colocalization analyses to investigate the effects of NPR2 signalling and NPR3 function on CVD outcomes and risk factors. MR estimates were compared to those obtained when considering height variants from throughout the genome.

Results
Genetically-proxied reduced NPR3 function was associated with a lower risk of CVD, with odds ratio (OR) 0.74 per standard deviation (SD) higher NPR3-predicted height, and 95% confidence interval (95% CI) 0.64–0.86. This effect was greater in magnitude than observed when considering height variants from throughout the genome. For CVD subtypes, similar MR associations for NPR3-predicted height were observed when considering the outcomes of coronary artery disease (0.75, 95% CI 0.60–0.92), stroke (0.69, 95% CI 0.50–0.95) and heart failure (0.77, 95% CI 0.58–1.02). Consideration of CVD risk factors identified systolic blood pressure (SBP) as a potential mediator of the NPR3-related CVD risk lowering. For stroke, we found that the MR estimate for NPR3 was greater in magnitude than could be explained by a genetically predicted SBP effect alone. Colocalization results largely supported the MR findings, with no evidence of results being driven by effects due to variants in linkage disequilibrium. There was no MR evidence supporting effects of NPR2 on CVD risk, although this null finding could be attributable to fewer genetic variants being identified to instrument this target.

Conclusions
This genetic analysis supports the cardioprotective effects of pharmacologically inhibiting NPR3 receptor function, which is only partly mediated by an effect on blood pressure. There was unlikely sufficient statistical power to investigate the cardioprotective effects of NPR2 signalling.
Electrochemical aptasensor based on the engineered core-shell MOF nanostructures for the detection of tumor antigens
Authors: Khan Suliman , Cho William C. , Sepahvand Afrooz , Haji Hosseinali Sara , Hussain Arif , Nejadi Babadaei Mohammad Mahdi , Sharifi Majid , Falahati Mojtaba , Jaragh-Alhadad Laila Abdulmohsen , ten Hagen Timo L. M. , Li Xin 
Published: April 2023

AbstractIt is essential to develop ultrasensitive biosensors for cancer detection and treatment monitoring. In the development of sensing platforms, metal-organic frameworks (MOFs) have received considerable attention as potential porous crystalline nanostructures. Core-shell MOF nanoparticles (NPs) have shown different diversities, complexities, and biological functionalities, as well as significant electrochemical (EC) properties and potential bio-affinity to aptamers. As a result, the developed core-shell MOF-based aptasensors serve as highly sensitive platforms for sensing cancer biomarkers with an extremely low limit of detection (LOD). This paper aimed to provide an overview of different strategies for improving selectivity, sensitivity, and signal strength of MOF nanostructures. Then, aptamers and aptamers-modified core-shell MOFs were reviewed to address their functionalization and application in biosensing platforms. Additionally, the application of core-shell MOF-assisted EC aptasensors for detection of several tumor antigens such as prostate-specific antigen (PSA), carbohydrate antigen 15-3 (CA15-3), carcinoembryonic antigen (CEA), human epidermal growth factor receptor-2 (HER2), cancer antigen 125 (CA-125), cytokeratin 19 fragment (CYFRA21-1), and other tumor markers were discussed. In conclusion, the present article reviews the advancement of potential biosensing platforms toward the detection of specific cancer biomarkers through the development of core-shell MOFs-based EC aptasensors.
“The familiar taste of poison”: a qualitative study of multi-level motivations for stimulant use in sexual minority men living in South Florida
Authors: Davis-Ewart Leah , Lee Ji-Young , Viamonte Michael , Colon-Burgos Josè , Harkness Audrey , Kanamori Mariano , Duncan Dustin T. , Doblecki-Lewis Susanne , Carrico Adam W. , Grov Christian 
Published: April 2023

Abstract
Background
In the US, stimulant use is associated with a 3–6 times greater rate of HIV seroconversion in sexual minority men (SMM) than in those who do not use stimulants. Annually, 1 in 3 SMM who HIV seroconvert will be persistent methamphetamine (meth) users. The primary objective of this qualitative study was to explore experiences of stimulant use in SMM living in South Florida, a high priority region for the Ending the HIV Epidemic initiative.

Methods
The sample included 25 SMM who use stimulants, recruited via targeted ads on social networking apps. Participants completed one-on-one semi-structured qualitative interviews, conducted from July 2019 through February 2020. A general inductive approach was used to identify themes relating to experiences, motivations, and overall relationship with stimulant use.

Results
Mean age of participants was 38.8, ranging from 20 to 61 years old. Participants were 44% White, 36% Latino, 16% Black and 4% Asian. Most participants were born in the US, self-identified as gay, and preferred meth as their stimulant of choice. Themes included: (1) stimulants as cognitive enhancements for focus or task completion, including transitioning to meth after first using prescription psychostimulants; (2) unique South Florida environment where participants could be open regarding their sexual minority status while also being influential on their stimulant use; (3) stimulant use as both stigmatizing and a coping mechanism for stigma. Participants anticipated stigma by family and potential sexual partners due to their stimulant use. They also reported using stimulants to cope with feelings of stigma due to their minoritized identities.

Conclusion
This study is among the first to characterize motivations for stimulant use in SMM living in South Florida. Results highlight both the risk and protective factors of the South Florida environment, psychostimulant misuse as a risk for meth initiation, and the role of anticipated stigma on stimulant use in SMM. Understanding stimulant use motivations can help to shape intervention development. This includes developing interventions that address individual, interpersonal, and cultural factors that drive stimulant use and increase risk of HIV acquisition.
Trial registration NCT04205487.
Association of oily fish and nonoily fish intakes with all-cause mortality and cause-specific mortality: a large population-based prospective study
Authors: Zhou Jian , Tan Xiankui , Li Yihan , Wan Rongjun , Wu Ziyi , Lin Zhengjun , Wang Wanchun , Luo Yingquan , Liu Tang 
Published: April 2023

Abstract
Background
There are inconsistent results of cohort studies analyzing the association between fish intake and mortality.

Objective
This study was performed to explore the association of oily fish consumption and nonoily fish consumption with all-cause mortality and cause-specific mortality.

Methods
A total of 431,062 participants from the UK Biobank who were without cancer or cardiovascular disease (CVD) at baseline between 2006 and 2010 were included in this study, and they were followed up through 2021. We constructed Cox proportional hazard models to calculate the hazard ratio (HR) and 95% confidence interval (CI) to assess the correlation of oily fish and nonoily fish intakes with mortality. Then, we performed subgroup analyses, and sensitivity analyses were developed and performed to examine the robustness of this study.

Results
Among the participants, 383,248 (88.9%) and 410,499 (95.2%) consumed oily fish and nonoily fish, respectively. Compared with the participants who did not consume oily fish, the adjusted HRs for the association of oily fish consumption (1 serving/week) with all-cause mortality and CVD mortality were 0.93 (0.87 to 0.98; p < 0.05) and 0.85 (0.74 to 0.98; p < 0.05), respectively. The multivariable-adjusted HRs of all-cause mortality for those who reported consuming < 1 serving/week of oily fish were 0.92 (0.86 to 0.98; p < 0.05).

Conclusion
Compared with participants who reported never consuming oily fish, the consumption of oily fish with 1 serving/week was more beneficial for all-cause and CVD mortality.
STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer
Authors: Li Jingxian , Qi Yuanjiong , Li Bo , Liu Yan , Yang Kuo , Zhang Zhihong , Zhu Jianqiang , Du E. 
Published: April 2023

Abstract
Background
The primary cilia (PC) is a microtubule-based and nonmotile organelle which protrudes from the surface of almost all mammalian cells. At present, PC has been found to be a deficiency or loss in multiple cancers. Restoring PC could be a novel targeting therapy strategy. Our research showed that PC was reduced in human bladder cancer (BLCA) cells, and PC deficiency promotes cell proliferation. However, the concrete mechanisms remain unknown. SCL/TAL1 interrupting locus (STIL), a PC-related protein, was screened in our previous study and could influence the cell cycle by regulating PC in tumor cells. In this study, we aimed to elucidate the function of STIL for PC to explore the underlying mechanism of PC in BLCA.

Methods
Public database analysis, western blot, and enzyme-linked immunosorbent assay (ELISA) were used to screen genes and explore gene expression alteration. Immunofluorescence and western blot were utilized to investigate PC. Wound healing assay, clone formation assay, and CCK-8 assay were used to explore cell migration, growth, and proliferation. The co-immunoprecipitation and western blot were employed to reveal the interaction of STIL and AURKA.

Results
We found that high STIL expression is correlated with poor outcomes of BLCA patients. Further analysis revealed that STIL overexpression could inhibit PC formation, activate SHH signaling pathways, and promote cell proliferation. In contrast, STIL-knockdown could promote PC formation, inactivate SHH signaling, and inhibit cell proliferation. Furthermore, we found that the regulatory functions of STIL for PC depend on AURKA. STIL could influence proteasome activity and maintain AURKA stabilization. AURKA-knockdown could reverse PC deficiency caused by STIL overexpression for PC in BLCA cells. We observed that co-knockdown in STIL and AURKA significantly enhanced PC assembly.

Conclusion
In summary, our result provides a potential therapy target for BLCA based on the restoration of PC.
A tumor focused approach to resolving the etiology of DNA mismatch repair deficient tumors classified as suspected Lynch syndrome
Authors: Walker Romy , Mahmood Khalid , Joo Jihoon E. , Clendenning Mark , Georgeson Peter , Como Julia , Joseland Sharelle , Preston Susan G. , Antill Yoland , Austin Rachel , Boussioutas Alex , Bowman Michelle , Burke Jo , Campbell Ainsley , Daneshvar Simin , Edwards Emma , Gleeson Margaret , Goodwin Annabel , Harris Marion T. , Henderson Alex , Higgins Megan , Hopper John L. , Hutchinson Ryan A. , Ip Emilia , Isbister Joanne , Kasem Kais , Marfan Helen , Milnes Di , Ng Annabelle , Nichols Cassandra , O’Connell Shona , Pachter Nicholas , Pope Bernard J. , Poplawski Nicola , Ragunathan Abiramy , Smyth Courtney , Spigelman Allan , Storey Kirsty , Susman Rachel , Taylor Jessica A. , Warwick Linda , Wilding Mathilda , Williams Rachel , Win Aung K. , Walsh Michael D. , Macrae Finlay A. , Jenkins Mark A. , Rosty Christophe , Winship Ingrid M. , Buchanan Daniel D. ,   for the Family Cancer Clinics of Australia
Published: April 2023

AbstractRoutine screening of tumors for DNA mismatch repair (MMR) deficiency (dMMR) in colorectal (CRC), endometrial (EC) and sebaceous skin (SST) tumors leads to a significant proportion of unresolved cases classified as suspected Lynch syndrome (SLS). SLS cases (n = 135) were recruited from Family Cancer Clinics across Australia and New Zealand. Targeted panel sequencing was performed on tumor (n = 137; 80×CRCs, 33×ECs and 24xSSTs) and matched blood-derived DNA to assess for microsatellite instability status, tumor mutation burden, COSMIC tumor mutational signatures and to identify germline and somatic MMR gene variants. MMR immunohistochemistry (IHC) and MLH1 promoter methylation were repeated. In total, 86.9% of the 137 SLS tumors could be resolved into established subtypes. For 22.6% of these resolved SLS cases, primary MLH1 epimutations (2.2%) as well as previously undetected germline MMR pathogenic variants (1.5%), tumor MLH1 methylation (13.1%) or false positive dMMR IHC (5.8%) results were identified. Double somatic MMR gene mutations were the major cause of dMMR identified across each tumor type (73.9% of resolved cases, 64.2% overall, 70% of CRC, 45.5% of ECs and 70.8% of SSTs). The unresolved SLS tumors (13.1%) comprised tumors with only a single somatic (7.3%) or no somatic (5.8%) MMR gene mutations. A tumor-focused testing approach reclassified 86.9% of SLS into Lynch syndrome, sporadic dMMR or MMR-proficient cases. These findings support the incorporation of tumor sequencing and alternate MLH1 methylation assays into clinical diagnostics to reduce the number of SLS patients and provide more appropriate surveillance and screening recommendations.
Cross‐cultural adaptation and validation of the Chinese version of the ankle joint functional assessment tool (AJFAT) questionnaire
Authors: Li Jinfeng , Qiu Fanji , Legerlotz Kirsten 
Published: April 2023

AbstractBackgroundAnkle joint functional assessment tool (AJFAT) is gradually becoming a popular tool for diagnosing functional ankle instability (FAI). However, due to the lack of standard Chinese versions of AJFAT and reliability and validity tests, the use of AJFAT in the Chinese population is limited. This study aimed to translate and cross‐culturally adapt the AJFAT from English into Chinese, and evaluate the reliability and validity of the Chinese version of AJFAT and to investigate its psychometric properties.MethodsThe translation and cross‐cultural adaptation of AJFAT was performed according to guidelines for cross‐cultural adaptation of self‐report measures. 126 participants with a history of ankle sprain completed the AJFAT‐C twice within 14 days and completed the Cumberland ankle instability tool (CAIT‐C) once. Test–retest reliability, internal consistency, ceiling and floor effects, convergent and structure validity and discriminative ability were investigated.ResultsThe test–retest reliability (ICC = 0.91, 95%CI = 0.87–0.94) and internal consistency (Cronbach's alpha = 0.87) of the AJFAT‐C were excellent. No ceiling or floor effects were detected. A moderate correlation between the AJFAT‐C and the CAIT‐C suggested a moderate convergent validity. The AJFAT‐C had a two‐factor structure: 1. function of the unstable side of the ankle joint (9 items) and 2. symptoms of the unstable side of the ankle (2 items). The ideal cut‐off point of the AJFAT‐C was calculated as 26 points.ConclusionThe Chinese version of AJFAT can be considered as a valid and reliable ankle joint function evaluation tool that can be applied in clinical and research work.
The impact of sewage effluents on water quality of Lake Hawassa, Ethiopia
Authors: Tibebe Dessie , Tesfaye Yemane , Kassa Yezbie 
Published: April 2023

AbstractThe physicochemical characteristics of water samples from Lake Hawassa was determined with the aim of pointing out possible impacts of industrial effluents, agricultural chemicals and domestic sewage on the water quality of the lake. For this, a total of 15 physicochemical parameters were measured in 72 water samples collected from four different locations on the lake that are adjacent to areas involved in various human activities including agriculture (Tikur Wuha), resort hotel (Haile Resort), public recreation (Gudumale) and referral hospital (Hitita). Samples were collected over a period of six months covering the dry and wet seasons in 2018/19. One-way analysis of variance revealed the presence of significant difference in the physicochemical quality of the lake’s water across the four study areas and the two seasons. Principal component analysis identified the most discriminating characteristics that differentiate the studied areas according to the nature and level of pollution status. Tikur Wuha area was found to be characterized by high levels of EC and TDS, the values of these parameters were about twice or more than that measured in the other areas. This was ascribed to contamination of the lake by runoff water from the surrounding farmlands. On the other hand, the water around the other three areas was characterized by high nitrate, sulfate and phosphate. Hierarchical cluster analysis classified the sampling areas in to two groups, where Tikur Wuha constituted one group and the other three locations the second group. Linear discriminant analysis provided 100% correct classification of the samples into the two cluster groups. The measured values of turbidity, fluoride and nitrate were found to be significantly higher than the standard limits set by national and international guidelines. These results show that the lake has been facing serious pollution problems from various anthropogenic activities.