bluuebunny/crossref_metadata_2025_split huggingface dataset:
Putative nucleotide-based second messengers in archaea
Authors: van der Does Chris , Braun Frank , Ren Hongcheng , Albers Sonja-Verena 
Published: June 2023

Abstract
Second messengers transfer signals from changing intra- and extracellular conditions to a cellular response. Over the last few decades, several nucleotide-based second messengers have been identified and characterized in especially bacteria and eukaryotes. Also in archaea, several nucleotide-based second messengers have been identified. This review will summarize our understanding of nucleotide-based second messengers in archaea. For some of the nucleotide-based second messengers, like cyclic di-AMP and cyclic oligoadenylates, their roles in archaea have become clear. Cyclic di-AMP plays a similar role in osmoregulation in euryarchaea as in bacteria, and cyclic oligoadenylates are important in the Type III CRISPR–Cas response to activate CRISPR ancillary proteins involved in antiviral defense. Other putative nucleotide-based second messengers, like 3′,5′- and 2′,3′-cyclic mononucleotides and adenine dinucleotides, have been identified in archaea, but their synthesis and degradation pathways, as well as their functions as secondary messengers, still remain to be demonstrated. In contrast, 3′-3′-cGAMP has not yet been identified in archaea, but the enzymes required to synthesize 3′-3′-cGAMP have been found in several euryarchaeotes. Finally, the widely distributed bacterial second messengers, cyclic diguanosine monophosphate and guanosine (penta-)/tetraphosphate, do not appear to be present in archaea.
Flexible Vis/NIR wireless sensing system for banana monitoring
Authors: Wang Meng , Wang Bingbing , Zhang Ruihua , Wu Zihao , Xiao Xinqing 
Published: June 2023

Abstract

Objectives
The quality of the fruit seriously affects the economic value of the fruit. Fruit quality is related to many ripening parameters, such as soluble solid content (SSC), pH, and firmness (FM), and is a complex process. Traditional methods are inefficient, do not guarantee quality, and do not adapt to the current rhythm of the fruit market. In this paper, a was designed and implemented for quality prediction and maturity level classification of Philippine Cavendish bananas.

Materials and Methods
The quality changes of bananas in different stages were analyzed. Twelve light intensity reflectance values for each maturity stage were compared to conventionally measured SSC, FM, PH, and color space.

Results
Our device can be compared with traditional forms of quality measurement. The experimental results show that the established predictive model with specific preprocessing and modeling algorithms can effectively determine various banana quality parameters (SSC, pH, FM, L*, a*, and b*). The RPD values of SSC and a* were greater than 3.0, the RPD values of L* and b* were between 2.5 and 3.0, and the pH and FM were between 2.0 and 2.5. In addition, a new banana maturity level classification method (FSC) was proposed, and the results showed that the method could effectively classify the maturity level classes (i.e. four maturity levels) with an accuracy rate of up to 97.5%. Finally, the MLR and FSC models are imported into the MCU to realize the near-range and long-range real-time display of data.

Conclusions
These methods can also be applied more broadly to fruit quality detection, providing a basic framework for future research.
Marination ingredients on meat quality and safety—a review
Authors: Ehsanur Rahman Syed Md , Islam Sharmeen , Pan Junyu , Kong Dewei , Xi Qian , Du Qijing , Yang Yongxin , Wang Jun , Oh Deog-Hwan , Han Rongwei 
Published: June 2023

Abstract
The practice of utilizing various categories of ingredients for marination has been prevalent in both home cooking and the meat industry for an extended period. Meat and meat products treated with various marination ingredients either alone or in combination with multiple marination processes can enhance color, flavor, and tenderness, while also improving their shelf life by reducing the growth of pathogenic microorganisms and lipid oxidation. This narrative review aims to examine all recent scientific literature on various meat and meat products subjected to marination. Through Google Scholar, PubMed, and Web of Science, the review summarizes all recent marinated and marination research articles, including types of marination ingredients, marination method, marinade mechanism, effect on sensory and nutritional quality, safety, shelf life, and health implications, resulting in a comprehensive overview of all information under marinades and marination for all scientists and food sectors concerned. The highlighted information will indicate future directions for the development of marination ingredients in the meat industry.
An angiotensin converting enzyme homolog is required for volatile pheromone detection, odorant binding protein secretion and normal courtship behavior in Drosophila melanogaster
Authors: Ha Tal Soo , Sengupta Samarpita , Powell Jordan , Smith Dean P 
Published: June 2023

Abstract
In many arthropods, including insects responsible for transmission of human diseases, behaviors that include mating, aggregation, and aggression are triggered by detection of pheromones. Extracellular odorant binding proteins are critical for pheromone detection in many insects and are secreted into the fluid bathing the olfactory neuron dendrites. In Drosophila melanogaster, the odorant binding protein LUSH is essential for normal sensitivity to the volatile sex pheromone, 11-cis vaccenyl acetate (cVA). Using a genetic screen for cVA pheromone insensitivity, we identified ANCE-3, a homolog of human angiotensin converting enzyme that is required for detection of cVA pheromone. The mutants have normal dose–response curves for food odors, although olfactory neuron amplitudes are reduced in all olfactory neurons examined. ance-3 mutants have profound delays in mating, and the courtship defects are primarily but not exclusively due to loss of ance-3 function in males. We demonstrate that ANCE-3 is required in the sensillae support cells for normal reproductive behavior, and that localization of odorant binding proteins to the sensillum lymph is blocked in the mutants. Expression of an ance-3 cDNA in sensillae support cells completely rescues the cVA responses, LUSH localization, and courtship defects. We show the courtship latency defects are not due to effects on olfactory neurons in the antenna nor mediated through ORCO receptors, but instead stem from ANCE-3-dependent effects on chemosensory sensillae in other body parts. These findings reveal an unexpected factor critical for pheromone detection with profound influence on reproductive behaviors.
A de novo missense variant in EZH1 associated with developmental delay exhibits functional deficits in Drosophila melanogaster
Authors: Jangam Sharayu V , Briere Lauren C , Jay Kristy L , Andrews Jonathan C , Walker Melissa A , Rodan Lance H , High Frances A ,   Undiagnosed Diseases Network, Yamamoto Shinya , Sweetser David A , Wangler Michael F 
Published: June 2023

Abstract
EZH1, a polycomb repressive complex-2 component, is involved in a myriad of cellular processes. EZH1 represses transcription of downstream target genes through histone 3 lysine27 (H3K27) trimethylation (H3K27me3). Genetic variants in histone modifiers have been associated with developmental disorders, while EZH1 has not yet been linked to any human disease. However, the paralog EZH2 is associated with Weaver syndrome. Here we report a previously undiagnosed individual with a novel neurodevelopmental phenotype identified to have a de novo missense variant in EZH1 through exome sequencing. The individual presented in infancy with neurodevelopmental delay and hypotonia and was later noted to have proximal muscle weakness. The variant, p.A678G, is in the SET domain, known for its methyltransferase activity, and an analogous somatic or germline mutation in EZH2 has been reported in patients with B-cell lymphoma or Weaver syndrome, respectively. Human EZH1/2 are homologous to fly Enhancer of zeste (E(z)), an essential gene in Drosophila, and the affected residue (p.A678 in humans, p.A691 in flies) is conserved. To further study this variant, we obtained null alleles and generated transgenic flies expressing wildtype [E(z)WT] and the variant [E(z)A691G]. When expressed ubiquitously the variant rescues null-lethality similar to the wildtype. Overexpression of E(z)WT induces homeotic patterning defects but notably the E(z)A691G variant leads to dramatically stronger morphological phenotypes. We also note a dramatic loss of H3K27me2 and a corresponding increase in H3K27me3 in flies expressing E(z)A691G, suggesting this acts as a gain-of-function allele. In conclusion, here we present a novel EZH1 de novo variant associated with a neurodevelopmental disorder. Furthermore, we found that this variant has a functional impact in Drosophila.
Volunteering and Changes in Cardiovascular Biomarkers: Longitudinal Evidence From the Health and Retirement Study
Authors: Kim Seoyoun , Halvorsen Cal , Han Sae Hwang 
Published: June 2023

Abstract

Background and Objectives
Growing body of research shows that volunteering is beneficial for those served, the volunteers, and the larger communities. However, major challenges remain that hinder the practical implications for volunteer activity as a public health intervention, including potential selection effects, lack of longitudinal studies that adjust for baseline characteristics, and a paucity of studies that consider multiple physical health outcomes in a single model.

Research Design and Methods
Data from 2006 to 2016 waves of the Health and Retirement Study (2006–2016) were used (N = 18,847). Outcome-wide analyses were utilized to evaluate if changes in volunteering between 2006/2008 (t0) and 2010/2012 (t1) were associated with 7 cardiovascular disease biomarkers 4 years later (2014/2016, t2). These models were adjusted for demographic factors, socioeconomic status, health behaviors, chronic conditions, baseline biomarkers, and volunteering. Additionally, selection into volunteering and attrition were taken into account.

Results
Compared with nonvolunteers, volunteering more than 200 hr a year was associated with a lower risk for clinically high diastolic blood pressure. In addition, increased volunteering effort (change from 1 to 99 hr at t0 to &gt;100 hr at t1) was associated with a lower likelihood of clinically high systolic and diastolic blood pressure levels. Sustained high volunteering (&gt;100 hr at both t0 and t1) was associated with lower diastolic blood pressure.

Discussion and Implications
The current study adds to the evidence on the health benefits of volunteering for adults 50 and older by inferring a potential causal link between high-intensity volunteering and reduced blood pressure.
Plate bending earthquakes and the strength distribution of the lithosphere
Authors: Sandiford Dan , Craig Timothy J 
Published: June 2023

SUMMARY
This study investigates the dynamics and constitutive behaviour of the oceanic lithosphere as it bends and yields during subduction. Two main observational constraints are considered: the maximum bending moment that can be supported by the lithosphere, and the inferred neutral plane depth in bending. We particularly focus on regions of old lithosphere where the ‘apparent’ neutral plane depth is about 30 km. We use subduction modelling approaches to investigate these flexural characteristics. We reassess bending moment estimates from a range of previous studies, and show a significant convergence towards what we call the ‘intermediate’ range of lithosphere strength: weaker than some classical models predict, but stronger than recent inferences at seamounts. We consider the non-uniqueness that arises due to the trade-offs in strength as well background (tectonic) stress state. We outline this problem with several end-member models, which differ in regard to relative strength in the brittle and ductile regimes. We evaluate the consistency of these models in terms of a range of constraints, primarily the seismic expression of the outer rise. We show that a 30 km neutral plane depth implies that net slab pull is not greater than about 2 TN m−1. In contrast, models with low brittle strength imply that regions with a 30 km neutral plane depth are under moderate net axial compression. Under these conditions, reverse faulting is predicted beneath the neutral plane at depths &gt;30 km. We show that moderate variations in background stress have a large impact on the predicted anelastic dissipation. We suggest brittle reverse faulting is a marginal phenomenon which may be inhibited by moderate changes in background stress.
Induced microseismicity and tremor signatures illuminate different slip behaviours in a natural shale fault reactivated by a fluid pressure stimulation (Mont Terri)
Authors: De Barros Louis , Guglielmi Yves , Cappa Frédéric , Nussbaum Christophe , Birkholzer Jens 
Published: June 2023

SUMMARYFault slip induced by fluid perturbation in shale formations may only lead to a sparse seismicity. However, fault slip may strongly impact the integrity of shale formations that serve as caprocks for geological reservoirs holding buoyant fluids such as CO2, natural gas or hydrogen. A better understanding of the fluid reactivation processes of fault and the seismic triggering process is therefore critical for reservoir monitoring and fault stability. Here we analyse the seismic responses of a shale fault exposed to fluid pressurization during an in situ field-scale injection experiment at ∼340 m depth in the Mont Terri underground research laboratory (Switzerland). Two main types of seismic signals are observed as the fault was activated and started to slowly slip. After an aseismic phase, we observed tremor signatures and an increase in noise amplitude, which were directly associated with the slowly propagating fault slip in response to fluid injection. These signatures were later followed by micro-earthquakes that seem to occur further away from the fluid-pressurized area. We interpret these micro-earthquakes to be triggered by stress perturbations from the main slip growth. These two classes of seismic responses therefore highlight two different processes. Tremors seem to be a more direct observation for the fluid-induced slip propagation than micro-earthquakes. Even hidden in the noise, they precede earthquake failures, thus providing a useful tool for monitoring fluid leakage activated by slow deformation on low permeable shale faults, with applications for sealing integrity of caprocks.
Simulating horizontal crustal motions of glacial isostatic adjustment using compressible Cartesian models
Authors: Reusen J M , Steffen R , Steffen H , Root B C , van der Wal W 
Published: June 2023

SUMMARY
Significant land uplift and horizontal motions have been recorded with Global Navigation Satellite Systems (GNSS) in areas such as Alaska, Iceland and the Northern Antarctic Peninsula (NAP) as a result of Glacial Isostatic Adjustment (GIA) due to ice melt after the Little Ice Age. Here, analysis of horizontal displacement rates can be of extra importance, as they are more sensitive to Earth properties in shallower layers than vertical displacement rates. Proper modelling of horizontal displacement rates with dedicated GIA models requires a spherical Earth with compressible rheology. However, in these small areas, the used GIA models are often incompressible using a Cartesian geometry to ease computation and in some cases allow for lateral viscosity changes or more complex rheology. We investigate the validity of modelled horizontal displacement rates using different approximations, that is using spherical or Cartesian Earth structures, and incompressible, material compressible or compressible rheology. Although the lack of self-gravity and sphericity compensate each other in the vertical, this is less the case for the horizontal. For a disc ice sheet with a radius just over 200 km and a thickness of 1000 m, differences due to sphericity are minimal and the modelled horizontal displacement rates of compressible Cartesian models differ from those simulated by a compressible spherical model by 0.63 mm a−1. Thus, compressible Cartesian GIA models can be applied for modelling horizontal displacement rates of small ice sheets like those in Alaska, Iceland and NAP. Unfortunately, the implementation of compressibility in Abaqus that we use here cannot be extended to spherical models as gravity can not be specified for a spherical body. Other modelling approaches are recommended in such cases.
Localized dissection of the sinus of Valsalva mimicking spontaneous coronary artery dissection
Authors: Yuge Norihisa , Manabe Susumu , Sugimura Koichiro , Shimokawa Hiroaki 
Published: June 2023

Abstract
A 56-year-old man, suspected of having ST-segment elevation myocardial infarction due to spontaneous coronary artery dissection, underwent emergency percutaneous coronary intervention. Although he had moderate aortic regurgitation with aortic root dilation and mild heart failure, it was controlled with medications. Two weeks after discharge, he was readmitted with severe heart failure due to severe aortic regurgitation and underwent an aortic root replacement. Intraoperative findings revealed that localized dissection of the sinus of Valsalva involved the right coronary artery, resulting in coronary artery dissection. In cases of spontaneous coronary artery dissection, attention should be paid to coronary artery dissection caused by localized aortic root dissection.
Urgent lung volume reduction surgery is effective for secondary pneumothorax in emphysema patients
Authors: Barbarossa Annalisa , Van Raemdonck Dirk , Weder Walter , Ceulemans Laurens J 
Published: June 2023

Abstract
Secondary pneumothorax due to emphysema can be life-threatening and requires surgery in most situations. Here, we extended lung resection to close the fistula using lung volume reduction surgery (LVRS). We present a patient with chronic obstructive pulmonary disease and secondary spontaneous pneumothorax referred after ineffective treatment by chemical pleurodesis. Urgent LVRS followed by elective LVRS was performed obtaining air-leak resolution and significantly improving pulmonary function and quality of life. We discuss the surgical technique and effectiveness of LVRS as a treatment for pneumothorax.
Minimax optimal clustering of bipartite graphs with a generalized power method
Authors: Braun Guillaume , Tyagi Hemant 
Published: June 2023

AbstractClustering bipartite graphs is a fundamental task in network analysis. In the high-dimensional regime where the number of rows $n_{1}$ and the number of columns $n_{2}$ of the associated adjacency matrix are of different order, the existing methods derived from the ones used for symmetric graphs can come with sub-optimal guarantees. Due to increasing number of applications for bipartite graphs in the high-dimensional regime, it is of fundamental importance to design optimal algorithms for this setting. The recent work of Ndaoud et al. (2022, IEEE Trans. Inf. Theory, 68, 1960–1975) improves the existing upper-bound for the misclustering rate in the special case where the columns (resp. rows) can be partitioned into $L = 2$ (resp. $K = 2$) communities. Unfortunately, their algorithm cannot be extended to the more general setting where $K \neq L \geq 2$. We overcome this limitation by introducing a new algorithm based on the power method. We derive conditions for exact recovery in the general setting where $K \neq L \geq 2$, and show that it recovers the result in Ndaoud et al. (2022, IEEE Trans. Inf. Theory, 68, 1960–1975). We also derive a minimax lower bound on the misclustering error when $K=L$ under a symmetric version of our model, which matches the corresponding upper bound up to a factor depending on $K$.
Regularity of Minimizing p-Harmonic Maps Into Spheres and Sharp Kato Inequality
Authors: Mazowiecka Katarzyna , Miśkiewicz Michał 
Published: June 2023

Abstract
We study regularity of minimizing $p$-harmonic maps $u \colon B^{3} \to \mathbb{S}^{3}$ for $p$ in the interval $[2,3]$. For a long time, regularity was known only for $p = 3$ (essentially due to Morrey [ 24]) and $p = 2$ (Schoen–Uhlenbeck [ 29]), but recently Gastel [ 7] extended the latter result to $p \in [2,2+\frac{2}{15}]$ using a version of Kato inequality. Here, we establish regularity for a small interval $p\in [2.961,3]$ by combining Morrey’s methods with Hardt and Lin’s Extension Theorem [ 11]. We also improve on the other result by obtaining regularity for $p \in [2,p_{0}]$ with $p_{0} = \frac{3+\sqrt{3}}{2} \approx 2.366$. In relation to this, we address a question posed by Gastel and prove a sharp Kato inequality for $p$-harmonic maps in two-dimensional domains, which is of independent interest.
Preexisting Immunity Does Not Prevent Efficacy of Vesicular Stomatitis Virus–Based Filovirus Vaccines in Nonhuman Primates
Authors: Marzi Andrea , Feldmann Friederike , O’Donnell Kyle L , Hanley Patrick W , Messaoudi Ilhem , Feldmann Heinz 
Published: June 2023

Abstract
Ebola virus (EBOV) and Marburg virus (MARV) made headlines in the past decade, causing outbreaks of human disease in previously nonendemic yet overlapping areas. While EBOV outbreaks can be mitigated with licensed vaccines and treatments, there is not yet a licensed countermeasure for MARV. Here, we used nonhuman primates (NHPs) previously vaccinated with vesicular stomatitis virus (VSV)–MARV and protected against lethal MARV challenge. After a resting period of 9 months, these NHPs were revaccinated with VSV-EBOV and challenged with EBOV, resulting in 75% survival. Surviving NHPs developed EBOV glycoprotein (GP)-specific antibody titers and no viremia or clinical signs of disease. The single vaccinated NHP succumbing to challenge showed the lowest EBOV GP-specific antibody response after challenge, supporting previous findings with VSV-EBOV that antigen-specific antibodies are critical in mediating protection. This study again demonstrates that VSVΔG-based filovirus vaccine can be successfully used in individuals with preexisting VSV vector immunity, highlighting the platform's applicability for consecutive outbreak response.