bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biotechnological response curve of the cyanobacterium Spirulina subsalsa to light energy gradient
Authors: Pistelli Luigi , Del Mondo Angelo , Smerilli Arianna , Corato Federico , Sansone Clementina , Brunet Christophe 
Published: February 2023

AbstractBackgroundMicroalgae represent a suitable and eco-sustainable resource for human needs thanks to their fast growth ability, together with the great diversity in species and intracellular secondary bioactive metabolites. These high-added-value compounds are of great interest for human health or animal feed. The intracellular content of these valuable compound families is tightly associated with the microalgal biological state and responds to environmental cues, e.g., light. Our study develops aBiotechnological response curvestrategy exploring the bioactive metabolites synthesis in the marine cyanobacteriumSpirulina subsalsaover a light energy gradient. The Relative Light energy index generated in our study integrates the red, green and blue photon flux density with their relative photon energy. TheBiotechnological response curvecombined biochemical analysis of the macromolecular composition (total protein, lipid, and carbohydrate content), total sterols, polyphenols and flavonoids, carotenoids, phenolic compounds, vitamins (A, B1, B2, B6, B9, B12, C, D2, D3, E, H, and K1), phycobiliproteins, together with the antioxidant activity of the biomass as well as the growth ability and photosynthesis.ResultsResults demonstrated that light energy significantly modulate the biochemical status of the microalgaSpirulina subsalsarevealing the relevance of the light energy index to explain the light-induced biological variability. The sharp decrease of the photosynthetic rate at high light energy was accompanied with an increase of the antioxidant network response, such as carotenoids, total polyphenols, and the antioxidant capacity. Conversely, low light energy favorized the intracellular content of lipids and vitamins (B2, B6, B9, D3, K1, A, C, H, and B12) compared to high light energy.ConclusionsResults of theBiotechnological response curveswere discussed in their functional and physiological relevance as well as for the essence of their potential biotechnological applications. This study emphasized the light energy as a relevant tool to explain the biological responses of microalgae towards light climate variability, and, therefore, to design metabolic manipulation of microalgae.
SGLT2 inhibitor versus carbohydrate-restricted isocaloric diet: reprogramming substrate oxidation in type 2 diabetes
Authors: Igarashi Hiroyuki , Uchino Hiroshi , Kanaguchi Momoko , Hisanaga Kaori , Sato Genki , Yoshikawa Fukumi , Furuta Masashi , Washizawa Naohiro , Usui Shuki , Miyagi Masahiko , Hirose Takahisa 
Published: February 2023

Abstract
Objective
Based on the whole-body energy metabolism and insulin action, the difference between increased excretion of carbohydrate in urine by SGLT2i and reduced same amount of oral carbohydrate intake are scarce. This study aimed to compare the effect of carbohydrate availability with reduced oral intake (carbohydrate-restricted isocaloric diet: CRIC diet) or lost in urine, as urinary glucosuria on sodium/glucose cotransporter-2 inhibitor (SGLT2i) treatment, focus on the insulin requirement and the macronutrient oxidation within insulin treated type 2 diabetes.

Methods
This is randomized 3-arm open-label prospective study. Subjects treated with titrated basal-bolus insulin regimen subsequent to three diet regimens, control diet (CON), administration of canagliflozin 100 mg/day to CON (SGLT2i), or CRIC diet, with a week admission to the endocrinology ward followed by 12 weeks outpatients’ management. The main outcome measures including the total insulin dose (TID) required to achieve euglycemia, fasting and postprandial energy expenditure (EE) and respiratory quotient (RQ) at 1-week and 12-week.

Results
We enrolled 23 patients with type 2 diabetes (male/female: 14/9, age: 53.6 ± 14.2 years, body mass index: 26.9 ± 4.8 kg/m2, HbA1c: 12.5 ± 1.6%). The TID was similar with CON and SGLT2i at both 1 and 12-weeks. Although comparable net carbohydrate availability in SGLT2i and CRIC groups, the TID was significantly higher in the CRIC (p = 0.02) compare to the SGLT2i at both 1 and 12-weeks. Fasting EE was similar in all groups, postprandial EE was significantly elevated in the SGLT2i and CRIC groups compared to the CON group (p = 0.03 and 0.04). Compare to the CON, lower basal fasting RQ (p = 0.049) and decreased delta-RQ (postprandial RQ/fasting RQ) indicated continuous lipid substrate utilization in the SGLT2i (p = 0.04) and CRIC (p = 0.03) groups.

Conclusion
The CRIC diet resulted in a similar fasting and postprandial EE and substrate oxidation compared to the SGLT2i. The increased insulin requirement in the CRIC diet indicates that a relatively highly lipid and protein consumption, compared to the SGLT2i and CON, may influence insulin requirement.
Effect of disturbance stimulation using a split-belt treadmill on a patient with cerebellar ataxia: a case report
Authors: Myojin Saho , Yasumura Hiroyuki , Takashiba Jun , Morioka Shu 
Published: February 2023

Abstract
Purpose
We present the case of a patient with cerebellar ataxia who was treated with walking practice using a split-belt treadmill with disturbance stimulation. The treatment effects were evaluated for improvements in standing postural balance and walking ability.

Case presentation
The patient was a 60-year-old Japanese male who developed ataxia after cerebellar hemorrhage. Assessment was performed using the Scale for the Assessment and Rating of Ataxia, Berg Balance Scale, and Timed Up-and-Go tests. A 10 m walking speed and walking rate were also assessed longitudinally. The obtained values were fit into a linear equation (y = ax + b), and the slope was calculated. This slope was then used as the predicted value for each period relative to the pre-intervention value. After removing the trend of the value for each period relative to the pre-intervention value, the amount of pre- to post-intervention change for each period was calculated to verify the intervention effect. Furthermore, to verify the changes in gait over time, a three-dimensional motion analyzer was used to analyze the pre- and post-intervention gait five times, and the results were kinematically compared.

Results
No significant pre- to post-intervention changes were observed in the Scale for the Assessment and Rating of Ataxia scores. Conversely, the Berg Balance Scale score, walking rate, and 10 m walking speed increased, and the Timed Up-and-Go score decreased in the B1 period, indicating a marked improvement from the predicted results based on the linear equation. For changes in gait determined using three-dimensional motion analysis, an increase in stride length was observed in each period.

Conclusion
The present case findings suggest that walking practice with disturbance stimulation using a split-belt treadmill does not improve inter-limb coordination, but contributes to improving standing posture balance, 10 m walking speed, and walking rate.
Dose-specific efficacy of adipose-derived mesenchymal stem cells in septic mice
Authors: Li Kui , Wang Tao , Li Rui , Xue Fulai , Zeng Guodan , Zhang Jingyao , Ma Yuan , Feng Li , Kang Y. James 
Published: February 2023

Abstract
Background
Mesenchymal stem cells (MSCs) therapy for sepsis has been extensively studied in the past decade; however, the treatment regimen and mechanism of action of MSCs remain elusive. Here, we attempted to understand the efficacy and mechanism of action of MSCs on rescuing mice with sepsis.

Methods
A mouse model of sepsis was produced by cecal ligation and puncture (CLP). Allogeneic adipose-derived MSCs (ADSCs) were administered by intravenous infusion at 6 h after CLP, and dose-related effects of ADSCs on these mice were determined by survival rate, histopathological changes, biochemical and coagulation parameters, bacterial load, and plasma levels of endotoxin and inflammatory cytokines. The tissue distribution of intravenously infused ADSCs in septic mice was investigated by pre-labeling ADSCs with the lipophilic membrane dye PKH26. RNA sequencing analysis was performed to assess the transcriptional changes in peripheral blood mononuclear cells (PBMCs) and the liver.

Results
A significant therapeutic effect of ADSCs at a dose of 2 × 107 cells/kg in septic mice was evidenced by a remarkable reduction in mortality (35.89% vs. 8.89% survival rate), blood bacterial burden, systemic inflammation, and multiple organ damage. In contrast, ADSCs at a lower dose (1 × 107 cells/kg) failed to achieve any beneficial outcomes, while ADSCs at a higher dose (4 × 107 cells/kg) caused more early death within 24 h after CLP, retaining a steady survival rate of 21.42% thereafter. PKH26-labeled ADSCs were predominantly localized in the lungs of septic mice after intravenous infusion, with only a smaller proportion of PKH26-positive signals appearing in the liver and spleen. RNA sequencing analysis identified that insufficient phagocytic activity of PBMCs in addition to a hyperactivation of the hepatic immune response was responsible for the ineffectiveness of low-dose ADSCs therapy, and acute death caused by high-dose ADSCs infusion was associated with impaired coagulation signaling in PBMCs and exacerbated hepatic hypoxic injury.

Conclusions
Our findings demonstrate a dose-specific effect of ADSCs on the treatment of sepsis due to dose-related interactions between exogenous stem cells and the host’s microenvironment. Therefore, a precise dosing regimen is a prerequisite for ADSCs therapy for sepsis.
Notes on molecular fragmentation and parameter settings for a dissipative particle dynamics study of a C10E4/water mixture with lamellar bilayer formation
Authors: Bänsch Felix , Steinbeck Christoph , Zielesny Achim 
Published: February 2023

AbstractThe influence of molecular fragmentation and parameter settings on a mesoscopic dissipative particle dynamics (DPD) simulation of lamellar bilayer formation for a C10E4/water mixture is studied. A “bottom-up” decomposition of C10E4 into the smallest fragment molecules (particles) that satisfy chemical intuition leads to convincing simulation results which agree with experimental findings for bilayer formation and thickness. For integration of the equations of motion Shardlow’s S1 scheme proves to be a favorable choice with best overall performance. Increasing the integration time steps above the common setting of 0.04 DPD units leads to increasingly unphysical temperature drifts, but also to increasingly rapid formation of bilayer superstructures without significantly distorted particle distributions up to an integration time step of 0.12. A scaling of the mutual particle–particle repulsions that guide the dynamics has negligible influence within a considerable range of values but exhibits apparent lower thresholds beyond which a simulation fails. Repulsion parameter scaling and molecular particle decomposition show a mutual dependence. For mapping of concentrations to molecule numbers in the simulation box particle volume scaling should be taken into account. A repulsion parameter morphing investigation suggests to not overstretch repulsion parameter accuracy considerations.
Big five model personality traits and job burnout: a systematic literature review
Authors: Angelini Giacomo 
Published: February 2023

Abstract
Background
Job burnout negatively contributes to individual well-being, enhancing public health costs due to turnover, absenteeism, and reduced job performance. Personality traits mainly explain why workers differ in experiencing burnout under the same stressful work conditions. The current systematic review was conducted with the PRISMA method and focused on the five-factor model to explain workers' burnout risk.

Methods
The databases used were Scopus, PubMed, ScienceDirect, and PsycINFO. Keywords used were: “Burnout,” “Job burnout,” “Work burnout,” “Personality,” and “Personality traits”.

Results
The initial search identified 3320 papers, from which double and non-focused studies were excluded. From the 207 full texts reviewed, the studies included in this review were 83 papers. The findings show that higher levels of neuroticism (r from 0.10** to 0.642***; β from 0.16** to 0.587***) and lower agreeableness (r from − 0.12* to − 0.353***; β from − 0.08*** to − 0.523*), conscientiousness (r from -0.12* to -0.355***; β from − 0.09*** to − 0.300*), extraversion (r from − 0.034** to − 0.33***; β from − 0.06*** to − 0.31***), and openness (r from − 0.18*** to − 0.237**; β from − 0.092* to − 0.45*) are associated with higher levels of burnout.

Conclusions
The present review highlighted the relationship between personality traits and job burnout. Results showed that personality traits were closely related to workers’ burnout risk. There is still much to explore and how future research on job burnout should account for the personality factors.
An empirical comparison of the performances of single structure columnar in-memory and disk-resident data storage techniques using healthcare big data
Authors: Famutimi R. F. , Oyelami M. O. , Ibitoye A. O. , Awoniran O. M. 
Published: February 2023

AbstractHealthcare data in images, texts and other unstructured formats have continued to grow exponentially while generating storage concerns. Even though there are other complexities, volume complexity is a major challenge for Disk-Resident technique in storage optimization. Hence, this research aimed to empirically compare the efficiency of Disk-Resident and In-Memory single structure database technique (as opposed to multiple structure In-Memory database), using descriptive and inferential big data analytical approaches. The essence was to discover a more cost-effective storage option for healthcare big data. Data from Nigerian Health Insurance Scheme (NHIS) alongside sample patients’ history from Made-in-Nigeria Primary Healthcare Information System (MINPHIS) which included patients’ investigation, patients’ bio-data and patients’ diagnoses were the primary data for this research. An implementation of both Disk-Resident and single structure In-Memory resident data storage was carried out on these big data sources. After storage, each quantity of data items stored for different data items in Disk-Resident was then compared with that of single structure In-Memory resident system using size of items as comparison criteria and different analyses made.The results obtained showed that single structure In-Memory technique conserved up to 90.57% of memory spaces with respect to the traditional (common) Disk-Resident technique for text data items. This shows that with this In-Memory technique, an improved performance in terms of storage was obtained.
Is one-stage lateral sinus lift and implantation safe in severely atrophic maxillae? Results of a comparative pilot study
Authors: Virnik Sascha , Cueni Laura , Kloss-Brandstätter Anita 
Published: February 2023

AbstractPurposeThe aim of this retrospective comparative study was to evaluate the survival of dental implants placed in the posterior maxilla with a residual bone height less than 3 mm using a one-stage lateral sinus lifting approach. The research question was whether in very severely atrophied maxillary bones (residual height < 3 mm), a sinus lift with simultaneous implant placement would be associated with a higher complication rate compared to single-stage sinus lifts at average residual alveolar process heights.MethodsComplications of 63 implants, where the residual bone height was below 3 mm, were compared to a reference group of 40 implants, which were inserted using a one-stage lateral sinus lift in maxillae with at least 3 mm residual bone height. Implant survival, bleeding-on-probing, the presence of peri-implant mucositis and the occurrence of peri-implantitis were documented.ResultsThe mean follow-up time for implant survival was 80.3 ± 25.9 months. One implant out of 63 was lost in the severely atrophic maxilla group and two implants out of 40 were lost in the reference group. There were no differences in the occurrence of implant loss (p = 0.558), bleeding-on-probing (p = 0.087), peri-implantitis (p = 0.999) and peri-implant mucositis (p = 0.797) between the severely atrophic alveolar ridge group and the reference group.ConclusionsEven in severely atrophic maxillae with < 3 mm residual bone height, a one-stage maxillary sinus lift and immediate implant placement can be carried out safely.Graphical Abstract
Microbiome response in an urban river system is dominated by seasonality over wastewater treatment upgrades
Authors: Kodera Sho M. , Sharma Anukriti , Martino Cameron , Dsouza Melissa , Grippo Mark , Lutz Holly L. , Knight Rob , Gilbert Jack A. , Negri Cristina , Allard Sarah M. 
Published: February 2023

Abstract
Background
Microorganisms such as coliform-forming bacteria are commonly used to assess freshwater quality for drinking and recreational use. However, such organisms do not exist in isolation; they exist within the context of dynamic, interactive microbial communities which vary through space and time. Elucidating spatiotemporal microbial dynamics is imperative for discriminating robust community changes from ephemeral ecological trends, and for improving our overall understanding of the relationship between microbial communities and ecosystem health. We conducted a seven-year (2013–2019) microbial time-series investigation in the Chicago Area Waterways (CAWS): an urban river system which, in 2016, experienced substantial upgrades to disinfection processes at two wastewater reclamation plants (WRPs) that discharge into the CAWS and improved stormwater capture, to improve river water quality and reduce flooding. Using culture-independent and culture-dependent approaches, we compared CAWS microbial ecology before and after the intervention.

Results
Examinations of time-resolved beta distances between WRP-adjacent sites showed that community similarity measures were often consistent with the spatial orientation of site locations to one another and to the WRP outfalls. Fecal coliform results suggested that upgrades reduced coliform-associated bacteria in the effluent and the downstream river community. However, examinations of whole community changes through time suggest that the upgrades did little to affect overall riverine community dynamics, which instead were overwhelmingly driven by yearly patterns consistent with seasonality.

Conclusions
This study presents a systematic effort to combine 16S rRNA gene amplicon sequencing with traditional culture-based methods to evaluate the influence of treatment innovations and systems upgrades on the microbiome of the Chicago Area Waterway System, representing the longest and most comprehensive characterization of the microbiome of an urban waterway yet attempted. We found that the systems upgrades were successful in improving specific water quality measures immediately downstream of wastewater outflows. Additionally, we found that the implementation of the water quality improvement measures to the river system did not disrupt the overall dynamics of the downstream microbial community, which remained heavily influenced by seasonal trends. Such results emphasize the dynamic nature of microbiomes in open environmental systems such as the CAWS, but also suggest that the seasonal oscillations remain consistent even when perturbed.
Development of antagonistic yeasts for controlling black mold disease of onion
Authors: Bagy Hadeel M. M. Kalil , Abo-Elyousr Kamal A. M. , Hesham Abd El-Latif , Sallam Nashwa M. A. 
Published: February 2023

Abstract
Background
The present study aimed to examine the biocontrol efficacy of 28 yeast isolates against Aspergillus niger, the common pathogen of onion black mold disease. The antagonistic potential of yeast isolates against A. niger growth was investigated using a dual culture technique.

Results
Five yeast isolates, including 8 and 11, showed the highest inhibition effect on the mycelial growth of A. niger. Molecular analysis using 16S RNA identified strains 8 (AUN-AH14) and 11 (AUN-AH23) as Galactomyces geotrichum (JQ713185.1) and G. geotrichum (DQ849321.1), respectively. The culture filtrates of AUN-AH14 and AUN-AH23 at percentage concentrations of 20, 40, 60, and 80 were tested against the growth of A. niger. As a result, the lowest dry weight of the pathogen was obtained with culture filtrates of the two strains at concentrations of 60 and 80%.. Treatment of onion bulbs with formulation of antagonistic yeast isolates AUN-AH14 and AUN-AH23 and their corresponding cultural filtrates at 80% concentration significantly reduced the severity of black mold disease relative to the controls.

Conclusions
The most significant reduction in black mold severity was proved in yeast formulations of AUN-AH14 and AUN-AH23, followed by their cultural filtrates (CF-AUN-AH14 and CF-AUN-AH23). The shelf life of formulated yeast isolates began to gradually deteriorate after 5 months of storage at 4 °C.
Effects of feeding Saccharomyces cerevisiae fermentation postbiotic on the fecal microbial community of Holstein dairy calves
Authors: Centeno-Martinez Ruth Eunice , Dong Wenxuan , Klopp Rebecca N. , Yoon Ilkyu , Boerman Jacquelyn P. , Johnson Timothy A. 
Published: February 2023

Abstract
Background
The livestock industry is striving to identify antibiotic alternatives to reduce the need to use antibiotics. Postbiotics, such as Saccharomyces cerevisiae fermentation product (SCFP), have been studied and proposed as potential non-antibiotic growth promoters due to their effects on animal growth and the rumen microbiome; however, little is known of their effects on the hind-gut microbiome during the early life of calves. The objective of this study was to measure the effect of in-feed SCFP on the fecal microbiome of Holstein bull calves through 4 months of age. Calves (n = 60) were separated into two treatments: CON (no SCFP added) or SCFP (SmartCare®, Diamond V, Cedar Rapids, IA, in milk replacer and NutriTek®, Diamond V, Cedar Rapids, IA, incorporated into feed), and were blocked by body weight and serum total protein. Fecal samples were collected on d 0, 28, 56, 84, and 112 of the study to characterize the fecal microbiome community. Data were analyzed as a completely randomized block design with repeated measures when applicable. A random-forest regression method was implemented to more fully understand community succession in the calf fecal microbiome of the two treatment groups.

Results
Richness and evenness of the fecal microbiota increased over time (P < 0.001), and SCFP calves tended to increase the evenness of the community (P = 0.06). Based on random-forest regression, calf age as predicted by microbiome composition was significantly correlated with the calf physiological age (R2 = 0.927, P < 1 × 10−15). Twenty-two “age-discriminatory” ASVs (amplicon sequence variants) were identified in the fecal microbiome that were shared between the two treatment groups. Of these, 6 ASVs (Dorea-ASV308, Lachnospiraceae-ASV288, Oscillospira-ASV311, Roseburia-ASV228, Ruminococcaceae-ASV89 and Ruminoccocaceae-ASV13) in the SCFP group reached their highest abundance in the third month, but they reached their highest abundance in the fourth month in the CON group. All other shared ASVs reached their highest abundance at the same timepoint in both treatment groups.

Conclusions
Supplementation of SCFP altered the abundance dynamics of age discriminatory ASVs, suggesting a faster maturation of some members of the fecal microbiota in SCFP calves compared to CON calves. These results demonstrate the value of analyzing microbial community succession as a continuous variable to identify the effects of a dietary treatment.