bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pleomorphic adenoma of the tongue: A case report
Authors: Liang Yuanzi , Bai Dong , Liu Wenting , Wang Zhiqun 
Published: March 2023

Rationale:
Salivary gland tumors account for approximately 3% of all tumors, most of which are benign, with pleomorphic adenomas being the most common, occurring mostly in middle-aged women, mostly originating from the major salivary glands and, to a lesser extent, from the minor salivary glands, with the tongue being a very rare site of occurrence. To date, case reports of pleomorphic adenoma at the root of the tongue are also rare.

Patient concerns:
A 56-year-old male patient with no obvious cause of foreign body sensation in the pharynx, sputum, no pain, no blood in the sputum, no dysphagia, and no difficulty in swallowing and breathing, which was significantly aggravated in the past 2 weeks, with difficulty in swallowing, breath-holding on lying down.

Diagnoses:
computed tomography and magnetic resonance imaging revealed a soft tissue mass at the root of the left tongue, which involved the tongue body in the forward direction. Electronic laryngopharyngoscopy showed a left-sided tongue root mass with a poorly smooth mucosa, covered with a mucous white pseudomembrane and a localized brownish-black crust without active bleeding. The final pathological findings showed a pleomorphic adenoma.

Interventions:
Postoperative symptomatic treatment was given, and the patient recovered well. Eight days after surgery, the patient was discharged from the hospital, and the pharyngeal pain basically subsided at the time of discharge, with no fever and no pharyngeal discomfort. Postoperative laryngoscopy showed smooth mucosa of the pharyngeal cavity, good pseudomembrane formation in the operated area, no active bleeding, no purulent secretions, and normal blood routine on recheck. The medical advice after discharge was firstly, full rest for 1 week, secondly, continue the oral anti-inflammatory treatment, 1 week after the operation need to review the outpatient clinic, finally, if there are any uncomfortable symptoms, seek medical attention in time.

Outcomes:
At present, the patient has been followed up for half a year and has recovered well from the operation without any discomfort.

Lessons:
It is very rare to find a pleomorphic adenoma of the tongue, and it occurs mostly in middle-aged women. In clinical diagnosis, it is sometimes difficult to distinguish it from malignant tumor of the tongue.
Multiple traditional Chinese medicinal approaches in collaboration with conservative conventional medicine for lumbar herniated intervertebral discs: A systematic review and meta-analysis
Authors: Kim Taeoh , Kim Hyungsuk , Shin Woo-Chul , Cho Jae-Heung , Song Mi-Yeon , Chung Won-Seok 
Published: March 2023

Background:
This review aimed to verify the clinical effects of traditional Chinese medicine in collaboration with conservative conventional medicine for lumbar herniated intervertebral discs (LHIVD).

Methods:
The CENTRAL, EMBASE, MEDLINE/PubMed, JMAS, CNKI, and seven Korean databases were searched using pre-planned strategies. The risk of bias was assessed using the Cochrane Collaboration tool and a meta-analysis was conducted accordingly.

Results:
Twenty-eight randomized controlled trials (RCTs) were included in this systematic review and meta-analysis. All RCTs included 2746 participants. The experimental group showed more significant improvements in VAS (Mean differences (MD) −1.61, 95% Confidence Intervals (CI) [−2.44, −0.79]; I
2 = 98%), Oswestry Disability Index (ODI) (MD −8.52, 95% CI [−11.71, −5.34]; I
2 = 96%), and Japanese Orthopaedic Association (JOA) scores (MD 4.83, 95% CI [3.49, 6.17]; I
2 = 85%) than the control group. However, the difference in the incidence of adverse events between the experimental and control groups was not significant (Relative Risk (RR) 0.05, 95% CI [0.23, 1.10]; I
2 = 46%).

Conclusion:
Traditional Chinese medicine in collaboration with conservative conventional medicine can be used to relieve pain and facilitate better function of the lumbar spine in lumbar herniated intervertebral disc cases. However, this conclusion should be applied with caution in clinical practice owing to the low quality of the included studies.
Biofeedback Training to Increase PCO2 in Asthma with Elevated Anxiety: A One-Stop Treatment for Both Conditions?
Authors: Meuret Alicia E. , Rosenfield David , Millard Mark M. , Ritz Thomas 
Published: March 2023

Abstract

Objective
Anxiety is highly prevalent in individuals with asthma. Asthma symptoms and medication can exacerbate anxiety, and vice versa. Unfortunately, treatments for comorbid anxiety and asthma are largely lacking. A problematic feature common to both conditions is hyperventilation. It adversely affects lung function and symptoms in asthma and anxiety. We examined whether a treatment to reduce hyperventilation, shown to improve asthma symptoms, also improves anxiety in asthma patients with high anxiety.

Method
One-hundred-twenty English- or Spanish-speaking adult patients with asthma were randomly assigned to either capnometry-assisted respiratory training (CART) to raise PCO2 or feedback to slow respiratory rate (SLOW). Although anxiety was not an inclusion criterion, 21.7% met clinically-relevant anxiety levels on the Hospital Anxiety and Depression scale. Anxiety (HADS-A) and depression (HADS-D) scales, anxiety sensitivity (ASI), and negative affect (PANAS-N) were assessed at baseline, posttreatment,1-month follow-up, and 6-month follow-up.

Results
In this secondary analysis, asthma patients with high baseline anxiety showed greater reductions in ASI and PANAS-N in CART than in SLOW (ps ≤ .005, Cohen's ds ≥ .58). Further, at 6-month follow-up, these patients also had lower ASI, PANAS-N, and HADS-D in CART than in SLOW (ps ≤ .012, Cohen's ds ≥ .54). Patients with low baseline anxiety did not have differential outcomes in CART than in SLOW.

Conclusions
For asthma patients with high anxiety, our brief training designed to raise PCO2 resulted in significant and sustained reductions in anxiety sensitivity and negative affect compared to slow-breathing training. The findings lend support for PCO2 as a potential physiological target for anxiety reduction in asthma.

Trial Registration:
clinicaltrials.gov Identifier: NCT00975273.
Trauma Across the Life Span and Multisystem Morbidity in Women With HIV
Authors: Rubin Leah H. , Maki Pauline M. , Dastgheyb Raha M. , Steigman Pamela J. , Burke-Miller Jane , Xu Yanxun , Jin Wei , Sosanya Oluwakemi , Gustafson Deborah , Merenstein Daniel , Milam Joel , Weber Kathleen M. , Springer Gayle , Cook Judith A. 
Published: March 2023

ABSTRACT

Objective
Sexual and physical abuse are highly prevalent among women living with HIV (WLWH) and are risk factors for the development of mental health and substance use disorders (MHDs, SUDs), and cognitive and medical comorbidities. We examined empirically derived patterns of trauma, MHD, and SUD, and associations with later cognitive and health outcomes.

Methods
A total of 1027 WLWH (average age = 48.6 years) in the Women’s Interagency HIV Study completed the World Mental Health Composite International Diagnostic Interview from 2010 to 2013 to identify MHDs, SUDs, and age at onset of sexual and physical abuse. Then, cognitive impairment, cardiovascular/metabolic conditions, and HIV disease outcomes were assessed for up to 8.8 years. Latent class analysis identified patterns of co-occurring trauma, MHDs, and/or SUDs. Generalized estimating equations determined associations between these patterns and midlife cognitive and medical outcomes.

Results
Six distinct profiles emerged: no/negligible sexual/physical trauma, MHD, or SUD (39%); preadolescent/adolescent sexual trauma with anxiety and SUD (22%); SUD only (16%); MHD + SUD only (12%); early childhood sexual/physical trauma only (6%); and early childhood sexual/physical trauma with later MHD + SUD (4%). Profiles including early childhood trauma had the largest number of midlife conditions (i.e., cognitive, cardiovascular, HIV-related). Preadolescent/adolescent sexual trauma with anxiety and SUD predicted both global and domain-specific cognitive declines. Only SUD without trauma predicted lower CD4, whereas childhood trauma with MHD + SUD predicted increased CD8.

Conclusions
WLWH have complex multisystem profiles of abuse, MHD, and/or SUD that predict midlife cognitive, metabolic/cardiovascular, and HIV outcomes. Understanding the interplay between these factors over time can identify risks and personalize preventative and treatment interventions.
Telehealth and Telemedicine in Latin American Rheumatology, a New Era After COVID-19
Authors: Graf Cesar , Fernández-Ávila Daniel G. , Plazzotta Fernando , Soriano Enrique R. 
Published: March 2023

Abstract
Telemedicine is the delivery of health care services by health care professionals using information and communication technologies to exchange valid information for the diagnosis, treatment, and prevention of diseases. Telemedicine was further developed in Latin America during the COVID-19 (coronavirus disease 2019) pandemic, becoming the first line of defense for health professionals to stop the spread of infections and allow them to continue the care of their patients. During the pandemic, 79% of rheumatologists in Latin America reported the use of remote communication, the most frequent being the use of phone calls and WhatsApp voice messages. In contrast, 84% of the patients reported that telemedicine was appropriate for them during the pandemic, but only 54% considered telemedicine to be a valid option for rheumatic health care after the pandemic. Telemedicine and telehealth have advantages such as lower costs, improved access in rural areas, shortage of care providers, and reduction in waiting time for appointments. However, it also has some challenges, such as legal, technological, and organizational barriers. In this review, we explore the current state of telemedicine in Latin America and discuss its future.
Stochastic model for cell population dynamics quantifies homeostasis in colonic crypts and its disruption in early tumorigenesis
Authors: Mamis Konstantinos , Zhang Ruibo , Bozic Ivana 
Published: March 2023

AbstractThe questions of how healthy colonic crypts maintain their size, and how homeostasis is disrupted by driver mutations, are central to understanding colorectal tumorigenesis. We propose a three-type stochastic branching process, which accounts for stem, transit-amplifying (TA) and fully differentiated (FD) cells, to model the dynamics of cell populations residing in colonic crypts. Our model is simple in its formulation, allowing us to estimate all but one of the model parameters from the literature. Fitting the single remaining parameter, we find that model results agree well with data from healthy human colonic crypts, capturing the considerable variance in population sizes observed experimentally. Importantly, our model predicts a steady state population in healthy colonic crypts for relevant parameter values. We show thatAPCandKRASmutations, the most significant early alterations leading to colorectal cancer, result in increased steady-state populations in mutated crypts, in agreement with experimental results. Finally, our model predicts a simple condition for unbounded growth of cells in a crypt, corresponding to colorectal malignancy. This is predicted to occur when the division rate of TA cells exceeds their differentiation rate, with implications for therapeutic cancer prevention strategies.
Pannexin 3deletion in mice results in knee osteoarthritis and intervertebral disc degeneration after forced treadmill running
Authors: Wakefield Brent , Hutchinson Jeffrey Lawrence , Tang Justin , Kanji Rehanna , Brooks Courtney , Séguin Cheryle A , Penuela Silvia , Beier Frank 
Published: March 2023

ABSTRACTPannexin 3 (Panx3) is a glycoprotein that forms mechanosensitive channels expressed in chondrocytes and annulus fibrosus cells of the intervertebral disc (IVD). Evidence suggestsPanx3plays contrasting roles in traumatic versus aging osteoarthritis (OA) and intervertebral disc degeneration (IDD). However, whether its deletion influences the response of joint tissue to mechanical stress is unknown. The purpose of this study was to determine ifPanx3deletion in mice causes increased knee joint OA and IDD after forced treadmill running. Male and female wildtype (WT) andPanx3knockout (KO) mice were randomized to either a no exercise group (sedentary; SED) or daily forced treadmill running (forced exercise; FEX) from 24 to 30 weeks of age. Knee cartilage, tibial secondary ossification center and IVD histopathology were evaluated by histology. Both male and femalePanx3KO mice developed larger superficial defects of the tibial cartilage after forced treadmill running compared to SED WT mice. Additionally, both male and femalePanx3KO mice developed greater bone area of the tibial secondary ossification center with running. In the lower lumbar spine, both male and femalePanx3KO mice developed histopathological features of IDD after running compared to SED WT mice. These findings suggest that the combination of deletingPanx3and forced treadmill running induces OA and causes histopathological changes associated with degeneration of the IVDs in mice.
Fabrication and Characterization of Ceramic-Polymer composite 3D scaffolds and Demonstration of Osteoinductive propensity with gingival Mesenchymal Stem Cells
Authors: Bahir Manjushree M , Rajendran Archana , Pattanayak Deepak , Lenka Nibedita 
Published: March 2023

AbstractBone tissue engineering involves the usage of metals, polymers, and ceramics as the base constituents in the fabrication of various biomaterial 3D scaffolds. Of late, the composite materials facilitating enhanced osteogenic differentiation/regeneration have been endorsed as the ideally suited bone grafts for addressing critical-sized bone defects. Here, we report the successful fabrication of 3D composite scaffolds with collagen type I (Col-I) in conjunction with three different crystalline phases of calcium-phosphate (CP) nanomaterials [hydroxyapatite (HAp), beta-tricalcium phosphate (βTCP), biphasic hydroxyapatite (βTCP-HAp or BCP)], obtained by altering the pH as the major variable. The fabricated 3D scaffolds consisting of ∼70 wt % CP nanomaterials and ∼ 30 Wt % of Col-I did mimic the ECM of bone tissue. The different Ca/P ratio and the orientation of CP nanomaterials in CP/Col-I composite scaffolds altered the microstructure, surface area, porosity, and mechanical strength of the scaffolds and also influenced the bioactivity, biocompatibility, and osteogenic differentiation of gingival-derived mesenchymal stem cells (gMSCs). The microstructure of CP/Col-I 3D scaffolds assessed by Micro-CT analysis revealed randomly oriented interconnected pores with pore sizes ranging from 80-250, 125-380, and 100-450µm respectively for βTCP/Col-I, BCP/Col-I, and HAp/Col-I scaffolds. Among these, the BCP/Col-I achieved the highest surface area (∼ 42.6 m2/g) and porosity (∼85%), demonstrated improved bioactivity and biocompatibility, and promoted osteogenic differentiation of gMSCs. Interestingly, the Ca2+ions (3 mM) released from scaffolds could also facilitate the osteocyte differentiation of gMSCssansosteoinduction. Collectively, our study has demonstrated the ECM mimicking biphasic CP/Col-I 3D scaffold as an ideally suited tissue-engineered bone graft.
Assessing genotype adaptability and stability in perennial forage breeding trials using random regression models for longitudinal dry matter yield data
Authors: Fernandes Filho Claudio Carlos , Lima Barrios Sanzio Carvalho , Santos Mateus Figueiredo , Rodrigues Nunes Jose Airton , do Valle Cacilda Borges , Jank Liana , Rios Esteban Fernando 
Published: March 2023

AbstractGenotype selection for dry matter yield (DMY) in perennial forage species is based on repeated measures over time. Repeated measurements in forage breeding trials generate longitudinal datasets that must be properly analyzed giving a useful interpretation in the genotype selection process. In this study, we have presented a random regression (RRM) approach for selecting genotypes based on longitudinal DMY data generated from ten breeding trials and three perennial species, alfalfa (Medicago sativaL.), guineagrass (Megathyrsus maximus), andbrachiaria (Urochloa spp.). We also proposed the estimation of adaptability based on the area under the curve and stability based on the curve coefficient of variation. Our results showed that RRM always approximated the (co)variance structure into an autoregressive pattern. Furthermore, RRM can offer useful information about longitudinal data in forage breeding trials, where the breeder can select genotypes based on their seasonality by interpreting reaction norms. Therefore, we recommend using RRM for longitudinal traits in breeding trials for perennial species.
OverexpressedMalat1Drives Metastasis through Inflammatory Reprogramming of Lung Adenocarcinoma Microenvironment
Authors: Martínez-Terroba Elena , de Miguel Fernando J. , Li Vincent , Robles-Oteiza Camila , Politi Katerina , Zamudio Jesse R. , Dimitrova Nadya 
Published: March 2023

AbstractMetastasis is the main cause of cancer deaths but the molecular events leading to metastatic dissemination remain incompletely understood. Despite reports linking aberrant expression of long noncoding RNAs (lncRNAs) with increased metastatic incidence, in vivoevidence establishing driver roles for lncRNAs in metastatic progression is lacking. Here, we report that overexpression of the metastasis-associated lncRNAMalat1(metastasis-associated lung adenocarcinoma transcript 1) in the autochthonous K-ras/p53 mouse model of lung adenocarcinoma (LUAD) is sufficient to drive cancer progression and metastatic dissemination. We show that increased expression of endogenousMalat1RNA cooperates with p53 loss to promote widespread LUAD progression to a poorly differentiated, invasive, and metastatic disease. Mechanistically, we observe thatMalat1overexpression leads to the inappropriate transcription and paracrine secretion of the inflammatory cytokine, Ccl2, to augment the mobility of tumor and stromal cellsin vitroand to trigger inflammatory responses in the tumor microenvironmentin vivo. Notably, Ccl2 blockade fully reverses cellular and organismal phenotypes ofMalat1overexpression. We propose thatMalat1overexpression in advanced tumors activates Ccl2 signaling to reprogram the tumor microenvironment to an inflammatory and pro-metastatic state.
A comprehensive map of hotspots of de novo telomere addition inSaccharomyces cerevisiae
Authors: Ngo Katrina , Gittens Tristen H. , Gonzalez David I. , Hatmaker E. Anne , Plotkin Simcha , Engle Mason , Friedman Geofrey A. , Goldin Melissa , Hoerr Remington E. , Eichman Brandt F. , Rokas Antonis , Benton Mary Lauren , Friedman Katherine L. 
Published: March 2023

AbstractTelomere healing occurs when telomerase, normally restricted to chromosome ends, acts upon a double-strand break to create a new, functional telomere. De novo telomere addition on the centromere-proximal side of a break truncates the chromosome but, by blocking resection, may allow the cell to survive an otherwise lethal event. We previously identified several sequences in the baker’s yeast,Saccharomyces cerevisiae, that act as hotspots of de novo telomere addition (termed Sites of Repair-associated Telomere Addition or SiRTAs), but the distribution and functional relevance of SiRTAs is unclear. Here, we describe a high-throughput sequencing method to measure the frequency and location of telomere addition within sequences of interest. Combining this methodology with a computational algorithm that identifies SiRTA sequence motifs, we generate the first comprehensive map of telomere-addition hotspots in yeast. Putative SiRTAs are strongly enriched in subtelomeric regions where they may facilitate formation of a new telomere following catastrophic telomere loss. In contrast, outside of subtelomeres, the distribution and orientation of SiRTAs appears random. Since truncating the chromosome at most SiRTAs would be lethal, this observation argues against selection for these sequences as sites of telomere addition per se. We find, however, that sequences predicted to function as SiRTAs are significantly more prevalent across the genome than expected by chance. Sequences identified by the algorithm bind the telomeric protein Cdc13, raising the possibility that association of Cdc13 with single-stranded regions generated during the response to DNA damage may facilitate DNA repair more generally.
Multi-layered genetic approaches to identify approved drug targets
Authors: Sadler Marie C. , Auwerx Chiara , Deelen Patrick , Kutalik Zoltán 
Published: March 2023

AbstractDrugs targeting genes that harbor natural variations associated with the disease the drug is in-dicated for have increased odds to be approved. Various approaches have been proposed to iden-tify likely causal genes for complex diseases, including gene-based genome-wide association stud-ies (GWAS), rare variant burden tests in whole exome sequencing studies (Exome) or integration of GWAS with expression/protein quantitative trait loci (eQTL-GWAS/pQTL-GWAS). Here, we compare gene-prioritization approaches on 30 common clinical traits and benchmarked their ability to recover drug target genes defined using a combination of five drug databases. Across all traits, the top pri-oritized genes were enriched for drug targets with odds ratios (ORs) of 2.17, 2.04, 1.81 and 1.31 for the GWAS, eQTL-GWAS, Exome and pQTL-GWAS methods, respectively. We quantified the perfor-mance of these methods using the area under the receiver operating characteristic curve as metric, and adjusted for differences in testable genes and data origins. GWAS performed significantly better (54.3%) than eQTL (52.8%) and pQTL-GWAS (51.3%), but not significantly so against the Exome ap-proach (51.7%vs52.8% for GWAS restricted to UK Biobank data). Furthermore, our analysis showed increased performance when diffusing gene scores on gene networks. However, substantial improve-ments in the protein-protein interaction network may be due to circularity in the data generation process, leading to the node (gene) degree being the best predictor for drug target genes (OR = 8.7, 95% CI = 7.3-10.4) and warranting caution when applying this strategy. In conclusion, we systematically as-sessed strategies to prioritize drug target genes highlighting promises and potential pitfalls of current approaches.
The SMART Text2FHIR Pipeline
Authors: Miller Timothy A. , McMurry Andrew J. , Jones James , Gottlieb Daniel , Mandl Kenneth D. 
Published: March 2023

AbstractObjectiveTo implement an open source, free, and easily deployable high throughput natural language processing module to extract concepts from clinician notes and map them to Fast Healthcare Interoperability Resources (FHIR).Materials and MethodsUsing a popular open-source NLP tool (Apache cTAKES), we create FHIR resources that use modifier extensions to represent negation and NLP sourcing, and another extension to represent provenance of extracted concepts.ResultsThe SMART Text2FHIR Pipeline is an open-source tool, released through standard package managers, and publicly available container images that implement the mappings, enabling ready conversion of clinical text to FHIR.DiscussionWith the increased data liquidity because of new interoperability regulations, NLP processes that can output FHIR can enable a common language for transporting structured and unstructured data. This framework can be valuable for critical public health or clinical research use cases.ConclusionFuture work should include mapping more categories of NLP-extracted information into FHIR resources and mappings from additional open-source NLP tools.