bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic loci underlying important shoot morphological traits of wild emmer wheat revealed by GWAS
Authors: Rahman Shanjida , Islam Shahidul , Wang Penghao , Sharma Darshan , Dowla Mirza , Nevo Eviatar , Zhang Jingjuan , Ma Wujun 
Published: June 2023

Abstract

Wild emmer wheat (WEW) played a central role in wheat evolution. During the long process of evolution, rapid alteration and sporadic genomic changes occurred in wheat resulting gene modifications and loss to some extent. WEW contains numerous genes that are absent in modern wheat gene pool, which might be useful for improving wheat. But, there is a lack of investigation in exploring genotype to phenotype associations in WEW. This study conducted genome wide association study (GWAS) on 19 shoot morphological traits and identified the genetic loci associated with several phenotypes from a collection of 263 WEW accessions using 90K SNP (single nucleotide polymorphism). A total of 11,393 SNP markers which passed the data quality screening, were used to conduct the GWAS analysis using a mixed linear model in TASSEL (Trait Analysis by Association, Evolution, and Linkage) software. A total of 857 significant MTAs (marker-trait association) were identified harbouring on all fourteen chromosomes, among which 81 were highly significant. On average, each significant MTA explained approximately 7% of phenotypic variance. The most significant MTAs were for tiller number, biomass, and some of yield related traits such as yield/plant and seed size. Putative candidate genes were also predicted for highly significant MTAs using the bioinformatics platform. The majority of the selected MTAs showed significant differences between alternative alleles for the corresponding phenotypes indicating their potential to be used in the breeding program. The genetic loci, contributing significantly to phenotypic variation, identified from this study will be useful in improving wheat morphological traits.
Influence of life events of pregnant women on depression: The mediating effects of anxiety and the moderating effects of social support
Authors: he Li , Su Mei , Bie Li , Ha Yingchun , Yu Jing , Yang Yujing , Cai Qing , Yao Haiyan , Zhao Junxiang , Wu Fangyuan 
Published: June 2023

Abstract
Objective
Explore the influence of life events of pregnant women on depression, with anxiety as a mediator and social support as moderates.
Methods
From January 2020 to December 2021, 1,767 valid data were collected through field questionnaires using convenient sampling method. The basic situation questionnaire, Maternal Life Events Scale (LESPW), Patient Health Questionnaire 9-item scale (PHQ-9), Generalized Anxiety Disorder 7-item scale (GAD-7) and Social Support Scale (SRSS) were used as the investigation tools. The survey objects were pregnant women who had established maternity records in a maternal and child health hospital in a certain area of China. The bias of common methods was evaluated by SPSS Harman single factor test. Maternal life events, anxiety, depression and social support levels were described by frequency and percentage, and Pearson correlation tests were used to measure correlations between variables. SPSS PROCESS macros (Model 4 and Model 8) were used to test the mediating and moderating effects of the model.
Results
Life events of pregnant women have a positive effect on depression, anxiety plays an intermediary role between life events of pregnant women and depression, social support and its dimension negatively regulate the relationship between life events of pregnant women and anxiety, social support to support use dimension regulates the relationship between life events of pregnant women and depression.
Conclusion
Maternal life events and anxiety are risk factors for depression, and social support is a protective factor for depression. Reducing life events and anxiety in pregnant women and enhancing social support can help reduce depression levels in pregnant women.
Unravelling anosmia, ageusia and clinical presentations in hospitalized Taiwanese patients with COVID-19: Insights and Implications
Authors: HSU JUNG LUNG , Liu Mei-Chuen , Tsau Po-Wei , Chung Fu-Tsai , Lin Shu-Min , Chen Mei-Lan , Ro Long-Sun 
Published: June 2023

Abstract
Objectives
The objective of this study was to investigate the clinical characteristics and outcomes of hospitalized COVID-19 patients presenting with anosmia and/or ageusia symptoms.
Methods
We conducted a retrospective observational study among 231 hospitalized patients with COVID-19 in Taiwan from April 2021 to July 2021. Detailed initial clinical symptoms, dyspnea grading, laboratory investigations, and admission outcomes were analyzed to elucidate the significance of anosmia and/or ageusia.
Results
Cough, fever, and dyspnea were the most common symptoms, while anosmia and/or ageusia accounted for only 8% of symptoms in hospitalized patients. Patients presenting with anosmia and/or ageusia had more severe initial clinical symptoms and comorbidities. A higher proportion of patients with anosmia and/or ageusia underwent initial endotracheal intubation and received emergency monoclonal antibody treatment for COVID-19 than those without these symptoms. However, there were no significant differences in the levels of inflammatory markers between the two groups.
Conclusion
Our study highlights the distinct clinical presentations of anosmia and/or ageusia in hospitalized COVID-19 patients. Anosmia and/or ageusia could be an important predictor of disease severity and may warrant early intervention in COVID-19 patients. Further studies are needed to confirm these findings
Climate action and gender equality matter most for China's sustainable development
Authors: Wu Chaoyang , Xing Qiang , Chen Fang , Liu Jianguo , Pradhan Prajal , Bryan Brett , Schaubroeck Thomas , Carrasco Luis Roman , Gonsamo Alemu , Li Yunkai , Chen Xiuzhi , Deng Xiangzheng , Albanese Andrea , Li Yingjie , Xu Zhenci 
Published: June 2023

Abstract
Rescuing Sustainable Development Goals (SDGs) from failing requires understanding their interactions networks, i.e., synergies and trade-offs, at national and especially sub-national levels, where SDGs were delivered. This understanding will help itendifying the key hurdles and opportunities to prioritize the 17 SDGs in a indivisible manner for a country. However, current research on SDG priorities at sub-national levels remains limited mainly due to difficulty in data collection. Here, we collect a unified annual dataset of 102 indicators covering national and 31 provinces in China over 2000–2020. We analyze the importance of the 17 SDGs at national, provincial and regional levels through synergy and trade-off networks. The key SDGs in trade-off (provincial: 12/31, regional: 1/6) differ more than synergy (provincial: 7/31, regional: 0). Nevertheless, combating climate change (SDG13) and improving gender equality (SDG5) are overall key hurdles for China to achieving 2030 agenda. Focusing on poverty eradication (SDG1) and increasing clean water and sanitation (SDG6) have highly compound positive effect. Our findings provide essential knowledge and insight on adopting common but diffrentiaetd SDGs priorities and balance mattering China’s sustainable development.
Ketosis Prevents Abdominal Aortic Aneurysm Rupture Through C-C Chemokine Receptor Type 2 Downregulation and Enhanced MMP Balance
Authors: Zayed Mohamed , Sastriques-Dunlop Sergio , Elizondo-Benedetto Santiago , Arif Batool , Meade Rodrigo , Zaghloul Mohamed , Luehmann Hannah , Heo Gyu , English Sean , Liu Yongjian 
Published: June 2023

Abstract

Abdominal aortic aneurysms (AAAs) are prevelant with aging, and AAA rupture is associated with high mortality. There is currently no effective medical therapy for AAA rupture. Previous work demonstrated that the monocyte chemoattractant protein (MCP-1) / C-C chemokine receptor type 2 (CCR2) axis critically regulates AAA inflammation, matrix-metalloproteinase (MMP) production, and extracellular matrix (ECM) stability. Here we similarly observed that Ccr2-/- mice have significantly reduced AAA expansion and rupture. We therefore hypothesized that a dietary modulation of the CCR2 axis may therapeutically impact AAA risk of rupture. Since ketone bodies (KBs) can trigger repair mechanisms in response to inflammation, we specifically evaluated whether systemic ketosis in vivo can reduce CCR2 and AAA progression. Male Sprague-Dawley rats underwent surgical AAA formation using porcine pancreatic elastase (PPE), and received daily β-aminopropionitrile (BAPN) to promote AAA rupture. Animals with AAAs received either a standard diet (SD), ketogenic diet (KD), or exogenous KBs (EKB). Animals recieving KD and EKB reached a state of ketosis, and had significant reduction in AAA expansion and incidence of rupture. Ketosis also led to significantly reduced aortic CCR2 content, improved MMP balance, and reduced ECM degradation. In summary, this study demonstrates that ketosis plays a crucial role in AAA pathobiology, and provides the impetus for future clinical studies investigating the potential benefit of ketosis for prevention of AAA expansion and rupture.
Aromatase inhibitors and fracture prevention – do 2017 guidelines work in real world?
Authors: Mirza Anem , Naing Zeyar Win , Khonsari Parisa , Khan Haseeb , Abbas Ali K. , Nisar Muhammad K 
Published: June 2023

Abstract

Objectives: Aromatase inhibitor induced bone loss (AIBL) is a recognised adverse event with resultant increase in fracture risk. We aimed to determine the real-world impact of the 2017 consensus guidelines on AIBL and it is effective in fracture prevention.
Methods: Over a 7-year study period, 1001 women prescribed AI were split in two groups. First group were offered bone active treatment based on NOS 2009 guidelines whereas the second group followed the 2017 consensus guidelines.
Results: 1001 women were included.
First group: 361 women had a baseline DEXA with 143 (40%) women had a normal DEXA, 174 (48%) had osteopenia and 44 (12%) had osteoporosis. Of the women with osteopenia, 44 (25%) women were offered treatment and 22 (13%) women had a fracture.
Second group: 640 women had a baseline DEXA with 216 (33%) women were normal, 322(50%) had osteopenia and 107 (17%) had osteoporosis. Of the women with osteopenia, 127 (39%) women were offered treatment and 8 (2.5%) women had a fracture.
Conclusions: Our study provides real world evidence of the success of 2017 consensus statement in lowering fracture risk. A significant reduction in fractures pre (13%) and post guidelines change (2.5%) was demonstrated (absolute risk reduction of 10.5%) which has implications for healthcare systems worldwide as we have demonstrated this approach can reduce morbidity.
KDM4B down-regulates ERα signaling independent on its demethylase activity in vascular calcification
Authors: Zhao Yue , Liu Fei , LV Yang , Lin Yanxia , Wang Chunyu , Wang Shengli , Zeng Kai , Zhou Baosheng , Lin Lin , Feng Jianwei , Sun Ge , Chang Xiaocen , Cao Mengsu , Hu Xihong , Kato Shigeaki , Tian Wen 
Published: June 2023

Abstract
Aims: Vascular Calcification (VC) is recognized as an independent predictor of cardiovascular events. Estrogen replacement was reported as protective treatment against vascular calcification in postmenopausal women, while it is controversial because of its potential carcinogenicity. ERα co-regulators have been putatively considered as potential therapeutic targets for ERα-related cancers. However, the modulation of ERα action and biological function of ERα co-regulators in vascular calcification are still elusive.
Methods and Results: KDM4B (Histone lysine demethylases 4B) was identified to be highly expressed in β-phosphoglycerol treated human and mouse aortic smooth muscle cells (ASMCs) and VitD3-overloaded mice during calcification by Western blot and immunofluorescence staining. Co-immunoprecipitation (Co-IP) and confocal immunofluorescence imaging were performed to show the association between KDM4B and ERa. Luciferase reporter assay demonstrated that KDM4B downregulated ERa-induced transactivation, and qPCR results showed that KDM4B depletion increased mRNA expression of endogenous ERa target gene; the results by co-IP showed that KDM4B associated with PRC2 complex and ERa. ChIP assay (Chromatin immunoprecipitation) demonstrated that KDM4B depletion decreased the recruitment of PRC2 (Polycomb repressive complex 2) complex to estrogen response element (ERE) regions, thereby down-regulating the level of H3K27me3. Finally, KDM4B-mediated enhancement of ASMCs calcification was attenuated by the estrogen treatment.
Conclusion: KDM4B inhibits ERα-induced transactivation independent of its JmjC enzyme active region. KDM4B is involved in vascular calcification via down-regulation of ERα action. KDM4B associates with PRC2 complex to be recruited to ERE element of ERα downstream target gene, thereby modulating histone H3K27me3 modification on the ERE region, suggesting KDM4B acts as a new potential therapeutic target for VC.
Electrospun Gelatin Nanofibers with Lumbrokinase for Accelerated Wound Healing: In Vitro and In Vivo Evaluations
Authors: Wang Wen-Ling , Lai Yi-Hui , Huang Chiung-Hua , Yao Chun-Hsu 
Published: June 2023

Abstract
Background
Wound healing is a highly complex and intricate biological process involving cellular and molecular events. This study aims to investigate the potential of gelatin nanofibers containing lumbrokinase (LK), a fibrinolytic enzyme derived from earthworms, fabricated through electrospinning as a novel therapeutic strategy for promoting wound healing.
Methods
This study determined the therapeutical concentration of lumbrokinase (LK) through in vitro cell proliferation assay, angiogenesis, and anti-inflammation assay. In addition, the co-culture experiment confirmed that the GLK membrane fabricated for one hour obtained good biocompatibility and could release effective drug concentrations for collagen production, angiogenesis, and anti-inflammatory effect. Finally, a rat model was utilized to evaluate the efficacy of GLK in skin wound healing.
Results
The results indicated that the GLK membrane has a noticeable wound-healing effect on the local wounds of rats. Moreover, it shortens wound healing time, reduces damage caused by inflammation, and increases collagen production, angiogenesis, and fibroblast proliferation and epithelialization.
Conclusion
The GLK membrane incorporating lumbrokinase exhibited promising potential as a wound dressing for enhancing wound healing, reducing inflammation, and promoting angiogenesis. The findings have the potential for developing advanced wound dressings with improved therapeutic outcomes. Furthermore, they may pave the way for clinical application in the future.
A Novel method of routing in multi-channel multi-radio wireless mesh networks
Authors: Tabatabaei Shayesteh , Mehbodniya Abolfazl 
Published: June 2023

Abstract
In mesh networks, wireless mesh nodes include routers and mesh clients. In a wireless mesh network, the use of single-radio nodes operating on a single channel will reduce the capacity of the mesh network, and equipping the nodes with multiple radios, this operation on a non-overlapping channel will greatly increase the capacity and available bandwidth of the network. If the number of radios used increases the number of channels that can be used for the nodes, it will cause the links to use the same channels and lead to interference between the links. In this paper, two algorithms are proposed for multi- channel mesh network for routing selection. The first proposed algorithm is to allocate channels in multi-radio and multi-channel wireless mesh networks with tree structure, colonial competition and clustering algorithms, using the available bandwidth criteria, distance to destination. The load density of each node tries to form high-rate multi-channel paths. In the second algorithm, each router determines its priority in selecting the channel by considering the amount of potential interference in its interference range. The performance of the proposed protocols was compared with CAMF protocol and FB_MR routing protocol. Network productivity rate, end-to-end latency, number of lost packets are among the criteria used to compare the performance of the proposed method. The simulation was performed on OPNET pseudo-emulator version 10.5. The simulation results show better performance of the proposed ICA_MR colonial competition protocol than the RLFB_MR reinforcement learning method and the CAMF protocol and the FB_MR routing protocol.
The anti-loosening characteristics of cortical bone trajectory screws and conventional pedicle screws under different bone quality conditions: a comparative study
Authors: Fengliang WU , Hui LI , Chunang PAN , Xiaogang MA , Jiu WANG , LI Ping , Chao PENG 
Published: June 2023

Abstract
Purpose: We aimed to compare the characteristics of anti-loosening and the pullout force between cortical bone trajectory (CBT) and conventional trajectory (CT) screws under different bone qualities.
Methods: Finite element models were established for CBT screws and CT screws in a single-segment L4 vertebra. Compression loads were applied to the models, followed by cyclic loading to simulate the screw loosening process resulting from nail channel expansion. The pullout force was then applied along the screw axis to simulate screw pullout, and pull-out force under different bone quality was calculated and compared.
Results: In the loosening test, screw channel expansion was significantly smaller in the CBT group. The CT screw channel volume expanded by 20.34% and 20.60% in healthy and osteoporotic bone, whereas the CBT screw channel volume only expanded by 0.20% and 0.19%. The pullout force of CBT screws was higher than that of CT screws in both healthy and osteoporotic bone (CBT, 1457.51 N and 1040.65 N; CT, 761.68 N and 461.36 N).
Conclusions: The loosening model combined with pullout testing can better simulate in vivo screw holding capacity. In different bone qualities, especially in osteoporotic bone, CBT screws have better anti-loosening and higher pullout strength than CT screws.
Design, Development and Analysis of Aperture monopole patch Antenna for UWB Communication with multiple notched capabilities
Authors: suraj priyadarshi 
Published: June 2023

Abstract
Present work illustrate CPW fed apertured monopole patch antenna having multiple interfering bands filtering capabilities. The proposed antenna contains three complementary improved inverted U-shaped slots on the radiating patch to filter out interfering signals namely WiMAX (3.3 to 3.6 GHz), C-band now extended for 5G application (3.7 GHz to 4.2 GHz) and WLAN (5.15 to 5.85 GHz) (combined lower 5.15–5.35 GHz and upper 5.725–5.825 GHz, WLAN). The ground plane is surrounded over the radiator with few modifications on the top corner to enhance impedance matching and hence to obtain optimized overall bandwidth. The projected antenna is made up on substrate of FR-4 (er=4.4) with height of 1.6mm and all the characteristics has been studied with VNA in anechoic chamber environment. The simulated and physically verified responses are in very close treaty to each other. Filtering capability is cross verified with ADS systematic software and lumped-equivalent circuit model also proposed with respective values of all the elements used. Time domain and current distribution analysis has also been undertaken to strengthen the functioning of antenna.
Argania spinosa (L.) essential oil: Phytochemical Characterization, Molecular Docking, Anti-staphylococcal, and Anti-pseudomonal Activity
Authors: Almuhayawi Mohammed S. , Alruhaili Mohammed H. , Gattan Hattan S. , Alharbi Mohanned Talal , Nagshabandi Mohammed K. , Hagagy Nashwa , Almuhayawi Saad M. , Jaouni Soad K. Al , Selim Samy , Alanazi Awadh , Alruwaili Yasir , Mostafa Ehab M. , Abdelgawad Mohamed A. , Elnosary Mohamed E. 
Published: June 2023

Abstract
Medicinal plants exemplify an infinite source of effective remedies and traditional thanks to the various active components they contain. Secondary metabolites current in plant extracts, for instance, essential oils, have significant pharmacological properties, including antimicrobic effects. This experimental investigation aims to determine Argan oil’s volatile compounds and exanimate their in vitro antimicrobial properties. In silico simulations, molecular docking and pharmacokinetics (absorption, distribution, metabolism, excretion, and toxicity (ADMET) and drug-likeness prediction revealed the processes underlying the in vitro biological possessions. Gas chromatography–mass spectrophotometry (GC-MS) was used for chemical screening of the main components of Argan oil. In silico molecular docking studies were applied as an attempt to investigate the ability of selected bioactive constituents of extra argan oil to act as effective against Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) isolated from ear Infections, aiming to study their ability to interact with protease protein of S. aureus (PDB id: 5MM8) and P. aeruginosa (PDB id: 7M1M) essential therapeutic target protein. GC/MS analysis showed that Argan oil components were 21 compounds such as Oleic Acid, Ethyl Oleate, 10-Octadecenoic acid, and methyl ester. All compounds docked with protease protein of S. aureus and P. aeruginosa, the docking result values ranged from − 5 to -9.4 kcal/mol for protease protein of S. aureus and from − 5.7 to -9.7 kcal/mol for protease protein of P. aeruginosa compared with reference ligands. On the other hand, the studied Gram-positive bacteria were more influenced by the oil extracts under study than the Gram-negative bacteria. MIC illustrated that Argan seed oil inhibits S. aureus and P. aeruginosa. MBC ranged from 0.4 to 0.7 mg/ml. These outcomes afford scientific authentication of the traditional usages of this plant and recommend its impending application as natural medications.
Determining new disulfidptosis-associated lncRNA signatures pertinent to breast cancer prognosis and immunological microenvironment
Authors: Zheng Yifan , Lin Yufeng , Zhang Yongcheng , Liu Shangjie , Yang Yongxia , Huang Wenbin 
Published: June 2023

Abstract
Purpose: Disulfidptosis, a novel form of cell death triggered by disulfide stress, could have significant implications in breast cancer (BC) pathogenesis. Despite this, the identification of disulfidptosis-related lncRNAs in BC remains has not been reported. Therefore, this study aimed to examine the prognostic potential of disulfidptosis-associated lncRNAs in BC.
Methods: RNA-seq data and clinical information of BC patients were obtained from the TCGA database. Co-expression analysis was performed to identify disulfidptosis-associated lncRNAs. Subsequently, a risk signature was developed through univariate Cox and LASSO analyses, and its predictive ability was validated. Additionally, the association between the risk signature and immune cell infiltration, immune function, tumor mutational burden (TMB）, and immune checkpoints was investigated. Finally, potential anticancer drugs associated with the risk signatures were predicted.
Results: A 10-lncRNA signature was established to stratify BC patients into high-risk and low-risk groups, where the high-risk group showed an unfavorable prognosis. This signature served as an independent prognostic factor in BC patients. Notably, the two subgroups displayed distinct mutation gene profiles, and the risk score exhibited a significant correlation with TMB. Furthermore, ssGSEA and immune checkpoint analysis revealed a significant association between the predictive signature and the immune status of BC patients. Finally, 55 potential anticancer drugs associated with the signature were identified.
CONCLUSIONS: We successfully established an independent prognostic signature of disulfidptosis-related lncRNAs in BC patients. This signature provides a solid basis for future investigations into the functional significance of disulfidptosis-associated lncRNAs in breast cancer.