bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cascade-amplification of melanoma-targeted radio-immunotherapy via fusogenic liposomes functionalized with multivariate-gated aptamer assemblies
Authors: Luo Zhong , Xue Rui , Ren Xijiao , Wang Shuang , Ge Xinyue , Yao Xuemei , Min Junxia , Li Menghuan , Wang Fudi , Li Liqi 
Published: July 2023

Abstract

Radio-immunotherapy exploits the immunostimulatory features of ionizing radiation (IR) to enhance antitumor effects and offers emerging opportunities for treating invasive tumor indications such as melanoma. However, insufficient dose deposition and immunosuppressive microenvironment (TME) of solid tumors limit its efficacy. To address these challenges, a cascade-amplification strategy based on multifunctional fusogenic liposomes (Lip@AUR-ACP-aptPD-L1) was reported. The liposomes were loaded with gold-containing Auranofin (AUR) and inserted with multivariate-gated aptamer assemblies (ACP) and PD-L1 aptamers in the lipid membrane, potentiating melanoma-targeted AUR delivery while transferring ACP onto cell surface through selective membrane fusion. AUR amplified IR-induced immunogenic death of melanoma cells to release antigens and damage-associated molecular patterns such as ATP for triggering adaptive antitumor immunity. AUR-sensitized radiotherapy also upregulated MMP-2 expression that combined with released ATP to cause AND-gate activation of ACP, thus triggering the in-situ release of CpG-based immunoadjuvants for stimulating dendritic cell-mediated T cell priming. Furthermore, AUR inhibited tumor-intrinsic ERK1/2-HIF-1α-VEGF signaling to suppress infiltration of immunosuppressive cells for fostering an anti-tumorigenic TME. This study offers an approach for solid tumor treatment in the clinics.
Green synthesis of pyrazolines as anti-inflammatory, antimicrobial and antioxidant agents
Authors: Pawar Kiran Prakash , Bhosale Sachin , Dhake Avinash , Shinde Nirmala 
Published: July 2023

Abstract
Green methods such as microwave technique and grinding technique have been found superior over the conventional method in view of reaction time and energy requirement. Pyrazoline derivatives , 2a-i and 3a-i, were synthesized both conventional heating and by microwave irradiation, the synthesized compounds were characterized by 1H NMR, 13C NMR, IR and mass spectral analyses. They were screened for their in vitro anti-inflammatory, antimicrobial and antioxidant activities. Antimicrobial activity was performed by Agar plate method and 2d & 2e were active against Gram negative bacteria E. coli and S. aureus, 3c and 3g showed antioxidant activity (IC50=48.13 and 46.03μg/ml),3c shows respectively anti-inflammatory activity at (EC50= 11.00±1.26 µg/mL) 3c ,3d and 3e showed good anti-inflammatory activity at concentration (EC50= 11.70±0.98 µg/mL) & 11.70±0.98 µg/mL respectively.
Simulation of Triple-bands LSPR based on Exposed-core MOF with Multilayered Nanoshell: Application in Multiplex RI Sensing
Authors: Yang Guan , Hu Chengyuan , Chen Meize , Yang Xianchao , Liu Yuhuai , Sun Xiaohong , Yao Jianquan 
Published: July 2023

Abstract
A localized surface plasmon resonance (LSPR) refractive index (RI) sensor based on exposed-core micro-structured optical fiber (EC-MOF) with a multilayered nanoshell (MNS) is designed. The triple-bands LSPR provided by the MNS, which consists of a gold core and a silver shell that separated by a dielectric layer, can realize the detection of three different targets simultaneously for the first time to our best knowledge. The novel characteristics of intense and distinct resonance peak at shorter wavelength is different from the higher order SPR modes, and the physical mechanism of MNS is illustrated by plasmon hybridization theory. The RI sensing ability is investigated at 1.33-1.42 in both wavelength and amplitude interrogations with sensitivities of 2082 nm/RIU and 202 RIU-1. Peak 1 exhibits ultrasharp LSPR with full width half maximum (FWHM) only 6 nm, leading to figure of merit (FOM) of 347/RIU, about 6-356 times higher than other similar works. The influences of MNS structure, number, position and length in EC-MOF are all analyzed in detail. Results indicate that the sensing characteristics can be optimized by adjusting the geometrical dimensions of MNS and the sensor exhibits relative strong tolerance, which show great potential for multiplex biosensing used in harsh environment.
Genetic differences among populations of Gynura procumbens (Lour.) Merr.
Authors: Yu Jiangfan , Tan Xinjian , Huang Dunyuan , Zhong Qiuping , Gu Ping , Zhou Youcheng , Cao Linqing , Wang Liyun , Wan Xirui , Chen Chuansong , Yuan Yaqi , Guo Hongyan , Yan Chao , Wang Jinfeng , Ge Xiaoning 
Published: July 2023

Abstract
Twenty-four populations of Gynura procumbensfrom different geographical regions were studied to determine the genetic differences using ITS and trnL-trnF sequences. The ITS sequence had an aligned length of 718 bp and a G+C content of 49.8%, with 5 variable sites and 3 parsimonious informative sites. The trnL-trnF sequence had an aligned length of 835 bp and a G+C content of 35.0%, with 6 variable sites and 3 parsimonious informative sites. The phylogenetic tree based on trnL-trnFsequences showed that all populations of Gynura procumbens clustered into one branch, indicating that this sequence has strong identifying abilities in genetic difference studies. Furthermore, the phylogenetic analysis using both ITS and trnL-trnF sequences showed similar results. Populations collected from Taiwan and Indonesia were grouped into one clade, while those collected from Guizhou, Guangxi, Fujian, and Guangdong shared a close relationship, corresponding to their geographical distribution pattern.
Industrial solid waste based layered double hydroxide composite with enhanced adsorption affinity to Congo red, Acid red, and Reactive red
Authors: Zhang Siqian , Lee Jaewon , Li Bin , Zhang Tianyang , Baeck Sung-Hyeon , Shim Sang Eun 
Published: July 2023

Abstract
The synthesis of hierarchically porous materials from industrial solid waste as catalysts or adsorbents has attracted much attention in environmental pollution control. The present work highlights the synthesis of a series of MgFeAl layered double hydroxide (LDH) with different ratios of Mg/(Fe + Al) from red mud and ferronickel slag under different pH to decolorize three red dye solutions, namely, Congo red (CR), acid red (AR), and reactive red (RR). The maximum adsorption capacity (386.1 mg.g–1) for CR was obtained by the MgFeAl-LDH sample with Mg/(Fe + Al) mole ratio of 2 synthesized under pH 10. The optimized synthesis parameters led to a high surface area and large pore volume of MgFeAl-LDH, thereby enhancing the mass transfer of adsorbate to the active sites of the adsorbent. Further, the MgFeAl-LDH sample was also efficient in removing AR (120.9 mg.g–1) and RR (114 mg.g–1). Moreover, the adsorption kinetics and isotherms of MgFeAl-LDH fitted well with the pseudo-second-order and Langmuir models, respectively. The MgFeAl-LDH system developed a valuable approach to utilize industrial waste for dye removal.
Heat stress and feeding effects on the mucosa-associated and digesta microbiome and their relationship to plasma and digesta fluid metabolites in the jejunum of dairy cows
Authors: Koch Franziska , Reyer Henry , Görs Solvig , Hansen Christiane , Wimmers Klaus , Björn Kuhla 
Published: July 2023

Abstract
The intestinal microbiota plays a pivotal role in digestive processes and maintains gut health and intestinal homeostasis. These functions may be compromised by increased environmental heat which in turn reduces feed intake and gut integrity, while activating the intestinal immune system. However, it is not known whether high ambient temperatures, causing heat stress (HS) to dairy cows, disturb the eubiosis of the microbial community and if so, to which extent the reduction in feed intake and the impairment of circulating and intestinal metabolites induced by HS account for the alterations of the jejunal microbiota. To address these questions, jejunal digesta, mucosa, and plasma samples from cows exposed to heat stress (HS: 28°C, temperature-humidity-index (THI) = 76, n = 10), or control conditions (CON: 16°C, THI = 60, n = 10), or pair-feeding (PF: 16°C, THI = 60, n = 10) for 7 days were collected. Digesta fluids were examined for pH, acetate, non-esterified fatty acids (NEFA), glucose, and lactate, whilst plasma metabolites were analyzed for glucose, lactate, beta-hydroxybutyrate (BHB), triglycerides, NEFA, creatinine and urea. The microbiota composition of digesta and mucosa samples were analyzed by 16S rRNA sequencing. The alpha diversity was higher in mucosa than digesta, but not affected by high ambient temperatures. However, the mucosa-associated microbiota appears more prone to ambient heat than the digesta microbiome. Furthermore, HS cows showed a greater mucosal abundance of Bifidobacteriaceae, Succinivibrionaceae UCG-001, Clostridia and a trend for Lactobacillus compared to CON cows. In the digesta, lower abundance of Colidextribacter and a trend for lower Lachnospiraceae UCG-008 were found in HS than CON cows. Several correlations between plasma or intestinal metabolites and microbiota were elucidated, including Methanobacteriaceae correlating positively with plasma BHB and digesta glucose concentrations. The shifts in the microbial profile of the small intestine give some implications for the adaptive responses under HS conditions. Moreover, the reduction in feed intake during HS had non-negligible effects on microbial diversity and the abundance of certain taxa, underpinning the importance of nutrient supply on maintaining intestinal homeostasis.
Assessment of Genetic Relation for Different Ploidy Levels of Aegilops and Triticum Possessing Different Genome-Bearing Species using Start Codon Target Marker
Authors: Ahmad Nariman Salih 
Published: July 2023

Abstract
The Fertile Crescent of Southwest Asia holds high genetic diversity of crop species of the grass tribe Triticeae, which provide staple food for the majority human population. Genetic diversity and their relationships were studied among 51 accessions from Aegilops and Triticum species from Iraqi Kurdistan. Eighteen SCoT markers were used to generate a total of 139 alleles. The discriminating power of the markers was assessed with polymorphism information content (PIC) and marker index (MI), that valued at 0.56 and 3.7, respectively. These results indicate the informativeness of the markers used for studying genetic diversity among the Aegilops and Triticum species. A high level of diversity was identified among the accession, to separate three distinct groups based on the SCoT data. All the accessions from different species with an ‘A’ genome were clustered together in a group, including Aegilops tauschii (possessing D genome). While the other Aegilops (U genome) were clustered in the other two groups. Analysis of molecular variance (AMOVA) partitioned 62% of variability among populations, while 38% of variation was considered between accessions within populations. Principal coordinate analysis (PCoA) for SCoT data showed that the first two components clarified 54.73% of the total variation, indicating consistent patterns of genetic relationships between the accessions. Genetic relationships inferred from UPGMA dendrogram analysis were matched with the PCoA, indicating that the grouping patterns of the accessions were in agreement with their botanical classifications. These results obtained could allow for future insight into wheat breeding programs.
Experiences of Nursing Students Who Are Victims of Dating Violence: a Qualitative Study
Authors: BARROSO-CORROTO Esperanza , LAREDO-AGUILERA José Alberto , COBO-CUENCA Ana Isabel , Carmona-Torres Juan Manuel 
Published: July 2023

Abstract
Background: Dating Violence (DV) is a type of Intimate Partner Violence that occurs between young people, and they are those behaviours that cause physical, sexual or psychological harm.
Objective/Aim: to know the experience of university students around dating violence.
Design and Methods: Qualitative study with a phenomenological approach was conducted through semi-structured individual interviews with nursing students’ victims of dating violence with the same starting categories. The public involve in this study were nursing students who freely agreed to participate in the interviews and gave their informed consent.
Reporting Method: the present study has adhered to relevant EQUATOR guidelines COREQ.
Results: Eleven nursing students participated, the sample was heterogeneous for gender and sexual diversity. Obtaining results about their experience with dating violence, manifestations of dating violence and cyber violence in their relationships, consequences, formal and informal help seeking and proposals for help as nursing students, among others.
Conclusion: Dating violence is a serious problem that seriously affects the victims and requires the creation of prevention programs. The experiences of university students about DV are mainly painful experiences, with serious consequences for those involved, needing help from their close environment and professional help to overcome the problems generated by their partners.
Implications: It is important due to the high prevalence of this phenomenon, also among nursing students, to provide key points to future health professionals and victims of dating violence on the correct way to act against violence due to lack of knowledge on the subject. This study clarifies the experiences of dating violence and how to offer help to victims from the informal and professional sphere.
Trial Registration: This study was approved by the Ethics Committee of Clinical Research of the Health Area of Talavera de la Reina (Toledo) with code 01/2021.
Primary Liver Rhabdomyosarcoma in a Burnt-out disease: An unusual presentation
Authors: Vadisetti Satya Niharika , Patkar Shraddha , Pai Trupti , Qureshi Sajid 
Published: July 2023

Abstract
Rhabdomyosarcoma is the most common soft tissue sarcoma in children. However, Primary hepatic RMS is rare and less than 20 cases are reported in literature. We present the case of a neonate who presented to us with a lumbar mass and lost follow-up during COVID pandemic. She came back to us with a Primary liver Rhabdomyosarcoma in the setting of a burnt out disease. In the setting of a diagnostic dilemma, we found histopathology as the cornerstone to diagnosis. Multimodality therapy based on the experience of similar case reports is the answer is such rare cases where specific guidelines are not yet defined.
Serum tau protein elevation in migraine: a cross-sectional case- control study
Authors: Overeem Lucas Hendrik , Raffaelli Bianca , Fleischmann Robert , Süße Marie , Vogelgesang Antje , Maceski Aleksandra Maleska , Papadopoulou Athina , Ruprecht Klemens , Su Wendy , Koch Mirja , Siebert Anke , Arkuszewski Michal , Tenenbaum Nadia , Kuhle Jens , Reuter Uwe 
Published: July 2023

Abstract
Background: Migraine is a disorder associated with neuropeptide release, pain and inflammation. Tau protein has recently been linked to inflammatory diseases and can be influenced by neuropeptides such as CGRP, a key neurotransmitter in migraine. Here, we report serum concentrations of total-tau protein in migraine patients and healthy controls.
Methods: In this cross-sectional study, interictal blood samples from n=92 patients with episodic migraine (EM), n=93 patients with chronic migraine (CM), and n=42 healthy matched controls (HC) were studied. We assessed serum total-tau protein (t-tau), and for comparison neurofilament light chain protein (NfL), glial fibrillary acidic protein (GFAP), and ubiquitin carboxy-terminal hydrolase L (UCH-L1) concentrations using the Neurology 4-plex kit, on a single molecule array HD-X Analyzer (Quanterix Corp Lexington, MA). Matched serum/cerebrospinal fluid (CSF) samples were used for post-hoc evaluations of a central nervous source of relevant findings. We applied non-parametric tests to compare groups and assess correlations.
Results: Serum t-tau concentrations were elevated in EM [0.320 (0.204 to 0.466) pg/mL] and CM [0.304 (0.158 to 0.406) pg/mL] patients compared to HC [0.200 (0.114 to 0.288) pg/mL] (p=0.002 vs. EM; p=0.025 vs. CM). EM with aura [0.291 (0.184 to 0.486 pg/mL); p= 0.013] and EM without aura [0.332 (0.234 to 0.449) pg/mL; p=0.008] patients had higher t-tau levels than HC but did not differ between each other. Subgroup analysis of CM with/without preventive treatment revealed elevated t-tau levels versus HC only in the non-prevention group [0.322 (0.181 to 0.463) pg/mL; p=0.009]. T-tau was elevated in serum (p=0.028) but not cerebrospinal fluid (p=0.76). In contrast to t-tau, all proteins associated with cell damage (NfL, GFAP, and UCH-L1), did not differ between groups.
Discussion: Migraine is associated with t-tau elevation in serum but not in the CSF. Our clinical study identifies t-tau as a new target for migraine research.
The association of dietary glycemic index and glycemic load with risk of irritable bowel syndrome
Authors: Reisian Mahnaz , Milajerdi Alireza , Keshteli Ammar Hassanzadeh , Abbasi Fatemeh , Haghighatdoost Fahimeh , Azadbakht Leila , Esmaillzadeh Ahmad , Adibi Peyman 
Published: July 2023

Abstract
Purpose
To date, no existing research has explored the potential relationship between dietary Glycemic Index (GI) and Glycemic Load (GL) and the risk of developing Irritable Bowel Syndrome (IBS). Our objective was to examine this association within a substantial sample of Iranian adults.
Method
This study was conducted on 3363 general public adults in Isfahan, Iran. A validated dish-based 106-item semi-quantitative food frequency questionnaire was used to examine dietary GI and GL. The presence of IBS was explored using a modified Persian version of the ROME III questionnaire. Totally, 22.2% (n = 748) of study participants had IBS.
Result
Participants with the highest dietary GI, had higher odds for IBS than those in the lowest category (OR: 1.34; 95% CI: 1.03–1.75). However, the association became non-significant after controlling for potential confounders (OR: 1.14; 95% CI: 0.81–1.61). A positive significant association was found between dietary GI and odds of IBS-constipation predominant (IBS-C) (OR: 1.96; 95% CI: 1.09–3.52), but not with IBS-diarrhea predominant (IBS-D) (OR: 0.78; 95% CI: 0.43–1.40).
Conclusions
No substantial link was observed between dietary glycemic load (GL) and the risk of overall irritable bowel syndrome (IBS) or its subtypes. However, a positive correlation was discovered between dietary GI and IBS with constipation (IBS-C).
Endophthalmitis caused by Burkholderia cepacia complex (BCC): Clinical characteristics, antibiotic susceptibilities, and treatment outcomes
Authors: Beca Flavius A , Sengillo Jesse D , Robles-Holmes Hailey K , Iyer Prashanth G , Miller Darlene , Yannuzzi Nicolas A , Flynn Harry W 
Published: July 2023

Abstract
Purpose To report the clinical characteristics, antibiotic susceptibilities, and review the literature of Burkholderia cepacia complex (BCC) associated endophthalmitis.Study design: Retrospective, observational case seriesMethods Clinical and microbiology records were reviewed for patients evaluated at the Bascom Palmer Eye Institute and diagnosed with culture-confirmed endophthalmitis due to BCC. Antibiotic susceptibility profiles were generated using standard microbiologic protocols via an automated VITEK system.Results Endophthalmitis associated with BCC was diagnosed in three patients. Infection occurred in the setting of post-penetrating keratoplasty (PKP), glaucoma filtering surgery, and suspected trauma. All isolates demonstrated in vitro susceptibility to ceftazidime and meropenem. Presenting visual acuity (VA) ranged from hand motion to light perception. Initial treatment strategies included intravitreal ceftazidime (2.25 mg/0.1 mL) and vancomycin (1.0 mg/0.1mL) injections with fortified topical antibiotics in 2 patients, and surgical debridement of a corneoscleral melt with patch graft along with both topical fortified antibiotics oral antibiotics in the third patient. In all 3 patients, there was no VA improvement at last follow-up, as 2 eyes ultimately underwent enucleation and 1 eye exhibited phthisis bulbi at last follow-up. BCC related endophthalmitis was reviewed among 13 reports. Treatment outcomes were generally poor and antibiotic resistance was common. These BCC isolates cases demonstrated broad resistance patterns, with susceptibilities to ceftazidime (58%), ciprofloxacin (53%), and gentamicin (33%).Conclusions Endophthalmitis caused by B. cepacia is a rare clinical entity with generally poor visual outcomes despite prompt treatment with appropriate antibiotics.
Relaxation of mitochondrial hyperfusion in the diabetic retina via N6-furfuryladenine confers neuroprotection regardless of glycaemic status.
Authors: Anderson Aidan , Alfahad Nada , Wimalachandra Dulani , Bouzinab Kaouthar , Rudzinska Paula , Wood Heather , Fazey Isabel , Xu Heping , Lyons Timothy J. , Barnes Nicholas M. , Narendran Parth , Lord Janet M. , Rauz Saaeha , Ganley Ian G. , Curtis Tim M. , Wallace Graham R. , Hombrebueno Jose R. 
Published: July 2023

Abstract
The recovery of mitochondrial quality control (MQC) may bring innovative solutions for neuroprotection, while also imposing an extraordinary challenge given the need of holistic approaches to restore mitochondrial dynamics (fusion/fission) and turnover (mitophagy and biogenesis). In Diabetic Retinopathy (DR) this therapeutic approach is further compounded by our lack of understanding of human retinal neurodegeneration, but also of how MQC processes interplay during disease progression. Here, we show for the first time that mitochondria remodel towards hyperfusion during retinal neurodegeneration in human and murine diabetes. Using Mito-QC reporter, we demonstrate that mitochondrial hyperfusion blunts the homeostatic turnover of mitochondria in diabetes, causing metabolic, neuronal, and neuro-inflammatory (glial) stress. By mimicking this mitochondrial remodelling in vitro, we also generated a drug discovery platform whereby glycosylated N6-furfuryladenine(s) (but not other PINK1-activators) restored mitochondrial turnover and cellular bioenergetics by relaxing hyperfusion in a controlled fashion. Oral administration of glycosylated N6-furfuryladenine restored mitochondrial turnover in the murine (Ins2Akita) diabetic retina, improving clinical correlates (electroretinogram and SD-OCT) and conferring human-relevant neuroprotection regardless of glycaemic status. Collectively, our study provides new translational insights for neuroprotection in the diabetic retina through the holistic recovery of MQC.
Comparitive Study Between Different Frequency Analysis Methods Over Tidal Data
Authors: Kumar Tanmay 
Published: July 2023

Abstract
Decomposition of tidal frequencies of port 8443970 Boston, Massachusetts1, USA, into sinusoidal components due to the lunar phase and rotation of the earth. Effect of the rotation of earth was apparent through a RFFT2,3 frequency decomposition and the highest contributing frequency was 2.0 times per day, following the sublunar / antipodal nature of tides; A lowpass Butterworth filter4,5 was applied to the mean normalized tidal data in order to gauge effect of lunar phase on magnitude of tides. The output data was passed through RFFT, the highest contributing frequency was found to be 0.06896 times per day, which corresponds roughly to twice a month, this matches with the 2 sets of neap and spring tide during a lunar cycle. The above process was repeated with Welch’s Method6 and the Lomb-Scargle method. All three of them, independently lead to the same result, adding further veracity to the analysis. A comparison between the efficiency of the 3 methods was done using a profiler.