bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogenetic relationships of Cuban and Caribbean Plumeria (Apocynaceae) based on the plastid genome
Authors: Tiernan Nichole , Nakamura Kyoko , Burns Christina , Jestrow Brett , Oviedo Prieto Ramona , Francisco-Ortega Javier 
Published: August 2023

Abstract
Plumeria L. (Apocynaceae) is a Neotropical genus mostly restricted to the Caribbean, which is cultivated in tropical gardens worldwide. Almost all ~27 species are endemic to the Caribbean Islands, except for four (Plumeria inodora Jacq., Plumeria obtusa L., Plumeria pudica Jacq., and Plumeria rubra L.) that are native to Central America and northern South America. Cuba harbours the largest diversity of the genus, with 13 endemic species. We present the first molecular phylogeny of the genus based on the nucleotide sequences of the complete plastid genome. Partial plastid genomes of 11 Plumeria species and one closely related species (Himatanthus sp.) were sequenced using long-PCR and next-generation sequencing. The recovered clades mostly grouped by single islands or closely neighboring islands. The phylogenetic relationships indicate patterns of adaptive radiation, with ecological shifts within these island-based clades and a west-to-east colonization route in the region. Plumeria rubra and Himatanthus sp. were resolved as sister to the rest of the genus. One unexpected finding was that P. pudica, a species restricted to the southern Caribbean Basin mainland, was recovered within the clade containing the Caribbean Island endemics, rendering the endemic group polyphyletic. Our study provides a phylogenetic framework for future studies that will need to include nuclear molecular markers and a more extensive sampling.
Asynchronous demographic dynamics of rodent community in low latitude Asia
Authors: Liu Gaoming , Shi Cheng-Min , Teng Huajing , Zhang Jian-Xu , Liu Quansheng 
Published: August 2023

Abstract
There is increasing evidence that demographic history and phylogeographic consequences of past climate changes unfolded locally and varied from region to region. Despite the high rodent species diversity and endemism in low latitude Asia, how they have responded to the past climatic fluctuations remains unexplored. In the present study, we trapped 253 murine individuals and sequenced their mitochondrial COI gene sequence. A total of ten species belonging to five genera were recognized through phylogenetic analyses. The results of divergence time estimation showed that the most common ancestors for all recognized species occurred in the Pleistocene. Signals of demographic expansion were detected in six species by at least one test and the events of sharp population size increase occurred asynchronously among species. The demographic expansion during the glaciation periods was corroborated by the expanded suitable distributional areas predicted by ecological niche modelling. The diversified demographic histories of rat communities in low-latitude Asia suggested that species might have responded to past regional environmental changes in an individualistic way.
Leaf morphology and anatomy of Schlechtendalia luzulifolia, a basal member of subfamily Barnadesioideae (Asteraceae)
Authors: Urtubey Estrella , Klusch María C , Stuessy Tod F 
Published: August 2023

Abstract
Based on molecular phylogenetic studies, Barnadesioideae have been proposed to be the basal subfamily of Asteraceae. This is a complex of 10 genera and 87 species distributed primarily along the Andean mountains, Patagonia, and into southern Brazil and Uruguay. Phylogenetic analyses have recovered all genera as monophyletic groups and have provided insights to their inter-relationships. Four generic clades have been substantiated: (1) Chuquiraga, Doniophyton, and Duseniella; (2) Dasyphyllum; (3) Barnadesia and Huarpea; and (4) Archidasyphyllum, Arnaldoa, and Fulcaldea. The remaining genus, the monospecific Schlechtendalia, has been an outlier in the subfamily, with some previous analyses recovering it as basal for the entire subfamily, and others showing it as sister to Barnadesia and Huarpea (with weak support) as well as to other genera. Recent massive sampling of loci has confirmed Schlechtendalia as the sister genus for the subfamily. Schlechtendalia luzulifolia has morphology atypical for Asteraceae. The capitula are loose aggregations of florets, and the leaves are long and strap shaped, more reminiscent of monocots. Morphological and anatomical investigations of the leaves reveal long, laminar blades with parallelodromous vascularization. The vesture is often with ‘barnadesioid trichomes’, especially towards the base of the plant, plus additional uniseriate trichomes consisting of 3 to many cells, newly reported for the subfamily. Some glandular trichomes with 2-4 short cells also occur. The transverse anatomy of the leaves reveals a single epidermal layer on both surfaces, which also contain the stomata (the leaf being amphistomatic). The mesophyll is undifferentiated; the vascular traces are surrounded by sclerenchyma that not only encircles the traces but also extends towards the epidermis and connects with it. The morphology and anatomy of the leaves of Schlechtendalia are divergent in comparison with other genera of the subfamily. Chuquiraga, Doniophyton, and Huarpea have leaf adaptations for survival in xeric habitats, such as dense pubescence, grey surfaces, and revolute margins. Schlechtendalia, in contrast, is adapted to a more mesic environment, especially near the Atlantic Ocean and along the Uruguay and La Plata rivers. The leaves are oriented upright, which correlates with undifferentiated mesophyll and stomata on both epidermal layers. The stem is an underground rhizome, an adaptation that permits survival during seasonal drought in the austral summer in Uruguay and adjacent regions. It is hypothesized that Schlechtendalia may have become adapted to more mesic environments in the Miocene prior to the rise of the Andes and development of the modern arid environments, into which many of the other genera of the subfamily subsequently radiated.
“Do We Stay or Do We Go?” The Impact of Anti-LGBTQ+ Legislation on the HIV Workforce in the South
Authors: Person Anna K , Terndrup Christopher P , Jain Mamta K , Kelley Colleen F 
Published: August 2023

Abstract
The human immunodeficiency virus (HIV) workforce continues to face a crisis, particularly in the southern United States. Adding to known issues of administrative burden and less competitive compensation, recent anti- lesbian, gay, bisexual, transgender and queer (LGBTQ+) legislation threatens the already strained HIV workforce. HIV care providers advocate for all aspects of their patient's lives, including those needing gender-affirming care. The recent legislative targets against transgender patients, which involves many people with HIV, will clearly add to the burden on individual HIV care providers and therefore the HIV workforce. Recruitment and retention efforts in states impacted by these laws will become increasingly difficult without advocacy for the patients we serve. The HIV workforce must work together with LGBTQ+ populations to address these recent laws and promote the well-being of all our patients and colleagues.
Nirmatrelvir Resistance—de Novo E166V/L50V Mutations in an Immunocompromised Patient Treated With Prolonged Nirmatrelvir/Ritonavir Monotherapy Leading to Clinical and Virological Treatment Failure—a Case Report
Authors: Zuckerman Neta S , Bucris Efrat , Keidar-Friedman Danielle , Amsalem Muriel , Brosh-Nissimov Tal 
Published: August 2023

Abstract
Resistance of SARS-CoV-2 to antivirals was shown to develop in immunocompromised individuals receiving remdesivir. We describe an immunocompromised patient who was treated with repeated and prolonged courses of nirmatrelvir and developed de-novo E166V/L50F mutations in the Mpro region. These mutations were associated with clinical and virological treatment failure.
Dual mechanical valve thrombosis resolution through thrombolytics: a case series
Authors: McGuire Colin , Yang Michael , Papolos Alexander , Rogers Toby , Kenigsberg Benjamin 
Published: August 2023

Abstract

Background
Prosthetic valve thrombosis (PVT) is a severe complication of mechanical valve replacement. Simultaneous thrombosis of multiple prosthetic valves is rare and is associated with worse outcomes. Treatment options include anticoagulation, thrombolysis, and redo operative valve replacement, with rare reports of adjunctive balloon valvuloplasty. There is limited evidence to guide therapeutic selection, specifically dosing, timing, and duration of thrombolysis. The following case series highlights the importance of successful thrombolytic management in dual PVT with high bleeding risk defined as a coagulopathy with an elevated international normalized ratio greater than 3 and New York Heart Association (NYHA) Class III and IV heart failure.

Case summary
We describe two patients with concomitant aortic and mitral PVT. Both patients presented in NYHA Class III and IV heart failure with different challenges to surgical treatments including high bleeding risk from coagulopathy and history of multiple prior sternotomies. After multi-disciplinary discussions, both patients underwent a combination of low-dose, slow, or ultra-slow infusion of tissue plasminogen activator, with a resolution of their dual PVT seen on cine-fluoroscopy imaging as freely moving mechanical leaflets and improvement of heart failure symptoms back to baseline NYHA Class II or lower.

Discussion
Prosthetic valve thrombosis is a complex medical condition requiring a multi-disciplinary team to evaluate the best course of treatment. A trial of pharmacologic thrombolysis is often the first attempted treatment for obstructive PVT, although surgery is recommended for patients with NYHA IV symptoms, or with contraindications to thrombolysis, including high bleeding risk. However, in patients with high bleeding risk and NYHA Class III and IV heart failure, especially with surgical contraindications, low-dose thrombolytics, with slow or ultra-slow infusions, may still be safe and effective treatment strategies for multi-valve PVT. Further research is needed to guide thrombolysis in multi-valve PVT.
Digest: Unveiling the role of chemical communication in lizard diversification
Authors: Román-Palacios Cristian 
Published: August 2023

Abstract
Although many studies looking at the role that sexual selection plays in macroevolution have focused on the involvement of visual and acoustic signals, the potential influence of chemical communication remains unexplored. Instead of focusing on well-studied instances of sexually selected traits, Murali et al. analyze chemical communication to test whether sexual selection could help explain large-scale species diversity patterns in lizards. Their results suggest that, at least in lizards, chemical communication is uncoupled from differential diversification dynamics across clades.
Praemonitus praemunitus: can we forecast and prepare for future viral disease outbreaks?
Authors: Sessions Zoe , Bobrowski Tesia , Martin Holli-Joi , Beasley Jon-Michael T , Kothari Aneri , Phares Trevor , Li Michael , Alves Vinicius M , Scotti Marcus T , Moorman Nathaniel J , Baric Ralph , Tropsha Alexander , Muratov Eugene N 
Published: August 2023

Abstract
Understanding the origins of past and present viral epidemics is critical in preparing for future outbreaks. Many viruses, including SARS-CoV-2, have led to significant consequences not only due to their virulence, but also because we were unprepared for their emergence. We need to learn from large amounts of data accumulated from well-studied, past pandemics and employ modern informatics and therapeutic development technologies to forecast future pandemics and help minimize their potential impacts. While acknowledging the complexity and difficulties associated with establishing reliable outbreak predictions, herein we provide a perspective on the regions of the world that are most likely to be impacted by future outbreaks. We specifically focus on viruses with epidemic potential, namely SARS-CoV-2, MERS-CoV, DENV, ZIKV, MAYV, LASV, noroviruses, influenza, Nipah virus, hantaviruses, Oropouche virus, MARV, and Ebola virus, which all require attention from both the public and scientific community to avoid societal catastrophes like COVID-19. Based on our literature review, data analysis, and outbreak simulations, we posit that these future viral epidemics are unavoidable, but that their societal impacts can be minimized by strategic investment into basic virology research, epidemiological studies of neglected viral diseases, and antiviral drug discovery.
The Association Between Ankle–Brachial Index and Daily Patterns of Physical Activity: Results From the Hispanic Community Health Study/Study of Latinos
Authors: Chiu Venus , Urbanek Jacek K , Wanigatunga Amal A , Allison Matthew A , Ballew Shoshana H , Mossavar-Rahmani Yasmin , Sotres-Alvarez Daniela , Gallo Linda C , Xue Xiaonan , Talavera Gregory A , Evenson Kelly R , Kaplan Robert C , Matsushita Kunihiro , Schrack Jennifer A 
Published: August 2023

Abstract

Background
Peripheral artery disease (PAD) is associated with lower physical activity but less is known about its association with daily patterns of activity. We examined the cross-sectional association between ankle–brachial index (ABI) and objectively measured patterns of physical activity among Hispanic/Latino adults.

Methods
We analyzed data from 7 688 participants (aged 45–74 years) in the Hispanic Community Health Study/Study of Latinos. ABI was categorized as low (≤0.90, indicating PAD), borderline low (0.91–0.99), normal (1.00–1.40), and high (&gt;1.40, indicating incompressible ankle arteries). Daily physical activity metrics derived from accelerometer data included: log of total activity counts (LTAC), total log-transformed activity counts (TLAC), and active-to-sedentary transition probability (ASTP). Average differences between ABI categories in physical activity, overall and by 4-hour time-of-day intervals, were assessed using linear regression and mixed-effects models, respectively.

Results
In Hispanic/Latino adults, 5.3% and 2.6% had low and high ABIs, respectively. After adjustment, having a low compared to a normal ABI was associated with lower volume (LTAC = −0.13, p &lt; .01; TLAC = −74.4, p = .04) and more fragmented physical activity (ASTP = 1.22%, p &lt; .01). Having a low ABI was linked with more fragmented physical activity after 12 pm (p &lt; .01). Having a high ABI was associated with lower volumes of activity (TLAC = −132.0, p = .03).

Conclusions
Having a low or high ABI is associated with lower and more fragmented physical activity in Hispanic/Latino adults. In adults with low ABI, physical activity is more fragmented in the afternoon to evening. Longitudinal research is warranted to expand these findings to guide targeted interventions for PAD or incompressible ankle arteries.
Worsened Ability to Engage in Social and Physical Activity During the COVID-19 Pandemic and Older Adults’ Mental Health: Longitudinal Analysis From the Canadian Longitudinal Study on Aging
Authors: Cosco Theodore D , Wister Andrew , Best John R , Riadi Indira , Kervin Lucy , Hopper Shawna , Basta Nicole E , Wolfson Christina , Kirkland Susan A , Griffith Lauren E , McMillani Jacqueline M , Raina Parminder 
Published: August 2023

Abstract

Background and Objectives
Restrictions implemented to mitigate the transmission of coronavirus disease 2019 (COVID-19) affected older adults’ ability to engage in social and physical activities. We examined mental health outcomes of older adults reporting worsened ability to be socially and physically active during the pandemic.

Research Design and Methods
Using logistic regression, we examined the relationship between positive screen for depression (10-item Center for Epidemiological Studies—Depression Scale) or anxiety (7-item Generalized Anxiety Scale) at the end of 2020 and worsened ability to engage in social and physical activity during the first 6–9 months of the pandemic among older adults in Canada. Interactions between ability to participate in social and physical activity and social participation pre-COVID (2015–2018) and physical activity were also examined. We analyzed data collected before and during the COVID pandemic from the Canadian Longitudinal Study on Aging, a nationally representative longitudinal cohort: pre-pandemic (2015–2018), COVID-Baseline survey (April to May 2020), and COVID-Exit survey (September to December 2020).

Results
Of the 24,108 participants who completed the COVID-Exit survey, 21.96% (n = 5,219) screened positively for depression and 5.04% (n = 1,132) for anxiety. Worsened ability to participate in social and physical activity was associated with depression (odds ratio [OR] = 1.85 [95% confidence interval {CI} 1.67–2.04]; OR = 2.46 [95% CI 2.25–2.69]), respectively, and anxiety (OR = 1.66 [95% CI 1.37–2.02] and OR = 1.96 [95% CI 1.68–2.30]). Fully adjusted interaction models identified a buffering effect of social participation and the ability to participate in physical activity on depression (χ2 [1] = 8.86, p = .003 for interaction term).

Discussion and Implications
Older adults reporting worsened ability to participate in social and physical activities during the COVID-19 pandemic had poorer mental health outcomes than those whose ability remained the same or improved. These findings highlight the importance of fostering social and physical activity resources to mitigate the negative mental health impacts of future pandemics or other major life stressors that may affect the mental health of older adults.
Theoretical study of low-GWP refrigerants in high-temperature heat pump systems
Authors: Zhou Dong , Meng Zhaofeng , Liu Yin , Dong Suiju , Zhang Fan , Ding Chuangchuang , Huo Ziheng , Wang Mingming 
Published: August 2023

Abstract
In this paper, the thermal performance of three low-global warming potential refrigerants R1224yd(Z), R1223zd(E) and R1336mzz(Z) were studied to replace R245fa in the high-temperature heat pump system. The results showed that the heating capacity of R1224yd(Z), R1223zd(E) and R1336mzz(Z) was 6, 12 and 88% less than that of R245fa, respectively. The coefficient of performance (COP) of R1224yd(Z) and R1223zd(E) was 2 and 1% higher than that of R245fa, respectively. However, the COP of R1336mzz(Z) was 5% less than that of R245fa. The difference in compressor discharge temperature between R1224yd(Z), R1223zd (E), R1336mzz(Z) and R245fa was about ±5°C. R1224yd(Z) is the best refrigerant to replace R245fa. R1223zd(E) is the second. R1336mzz(Z) cannot be directly replaced unless the original system is modified.
Perspectives on Advancing Countermeasures for Filovirus Disease: Report From a Multisector Meeting
Authors: Sprecher Armand , Cross Robert , Marzi Andrea , Martins Karen A , Wolfe Daniel , Montgomery Joel M , Spiropoulou Christina F , Cihlar Tomas , Ahuka-Mundeke Steve , Nyhuis Tara , Teicher Carrie , Crozier Ian , Strong Jim , Kobinger Gary , Woolsey Courtney , Geisbert Thomas W , Feldmann Heinz , Muyembe Jean-Jacques 
Published: August 2023

AbstractAlthough there are now approved treatments and vaccines for Ebola virus disease, the case fatality rate remains unacceptably high even when patients are treated with the newly approved therapeutics. Furthermore, these countermeasures are not expected to be effective against disease caused by other filoviruses. A meeting of subject-matter experts was held during the 10th International Filovirus Symposium to discuss strategies to address these gaps. Several investigational therapeutics, vaccine candidates, and combination strategies were presented. The greatest challenge was identified to be the implementation of well-designed clinical trials of safety and efficacy during filovirus disease outbreaks. Preparing for this will require agreed-upon common protocols for trials intended to bridge multiple outbreaks across all at-risk countries. A multinational research consortium including at-risk countries would be an ideal mechanism to negotiate agreement on protocol design and coordinate preparation. Discussion participants recommended a follow-up meeting be held in Africa to establish such a consortium.
Modeling Poliovirus Transmission and Responses in New York State
Authors: Thompson Kimberly M , Kalkowska Dominika A , Routh Janell A , Brenner I Ravi , Rosenberg Eli S , Zucker Jane R , Langdon-Embry Marisa , Sugerman David E , Burns Cara C , Badizadegan Kamran 
Published: August 2023

Abstract

Background
In July 2022, New York State (NYS) reported a case of paralytic polio in an unvaccinated young adult, and subsequent wastewater surveillance confirmed sustained local transmission of type 2 vaccine-derived poliovirus (VDPV2) in NYS with genetic linkage to the paralyzed patient.

Methods
We adapted an established poliovirus transmission and oral poliovirus vaccine evolution model to characterize dynamics of poliovirus transmission in NYS, including consideration of the immunization activities performed as part of the declared state of emergency.

Results
Despite sustained transmission of imported VDPV2 in NYS involving potentially thousands of individuals (depending on seasonality, population structure, and mixing assumptions) in 2022, the expected number of additional paralytic cases in years 2023 and beyond is small (less than 0.5). However, continued transmission and/or reintroduction of poliovirus into NYS and other populations remains a possible risk in communities that do not achieve and maintain high immunization coverage.

Conclusions
In countries such as the United States that use only inactivated poliovirus vaccine, even with high average immunization coverage, imported polioviruses may circulate and pose a small but nonzero risk of causing paralysis in nonimmune individuals.
Reporting England’s progress towards the ambitions in the UK action plan for antimicrobial resistance: the English surveillance programme for antimicrobial utilisation and resistance (ESPAUR)
Authors: Ashiru-Oredope Diane , Cunningham Neil , Casale Ella , Muller-Pebody Berit , Hope Russell , Brown Colin S , Hopkins Susan 
Published: August 2023

Abstract
The plans for a new antimicrobial utilization and resistance national surveillance programme, alongside the development of quality measures and methods to monitor unintended outcomes of antimicrobial stewardship and both public and professional behaviour interventions were published in 2013. Since then, England has published an annual surveillance report including outlining progress against the ambitions of the UK national action plans on antimicrobial resistance (2013 to 2018 and 2019 to 2024). A decade later we provide a brief update on progress so far, with a focus on key highlights from the latest report published in November 2022. We also provide our recommendations for areas of focus as we move into the next decade. From an initial focus on antibiotic consumption and resistance, the report now includes surveillance data for antifungals, antivirals (including novel agents, such as those targeting SARS-CoV-2) and antimalarials. Evaluation of key stewardship interventions including professional and public engagement initiatives are also reported, as well as progress against NHS England’s (NHSE’s) improvement measures.