bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-differences in prostaglandin signaling: a semi-systematic review and characterization of PTGDS expression in human sensory neurons
Authors: Shen Breanna Q. , Sankaranarayanan Ishwarya , Price Theodore J. , Tavares-Ferreira Diana 
Published: March 2023

AbstractThere is increasing evidence of sex differences in underlying mechanisms causing pain in preclinical models, and in clinical populations. There are also important disconnects between clinical pain populations and the way preclinical pain studies are conducted. For instance, osteoarthritis pain more frequently affects women, but most preclinical studies have been conducted using males in animal models. The most widely used painkillers, nonsteroidal anti-inflammatory drugs (NSAIDs), act on the prostaglandin pathway by inhibiting cyclooxygenase (COX) enzymes. The purpose of this study was to analyze the preclinical and clinical literature on the role of prostaglandins and COX in inflammation and pain. We aimed to specifically identify studies that used both sexes and investigate whether any sex-differences in the action of prostaglandins and COX inhibition had been reported, either in clinical or preclinical studies. We conducted a PubMed search and identified 369 preclinical studies and 100 clinical studies that matched our inclusion/exclusion criteria. Our analysis shows that only 17% of preclinical studies on prostaglandins used both sexes and, out of those, only 19% analyzed or reported data separated by sex. In contrast, 79% of the clinical studies analyzed used both sexes. However, only 6% of those reported data separated by sex. Interestingly, 14 out of 15 preclinical studies and 5 out of 6 clinical studies that analyzed data separated by sex have identified sex-differences. This builds on the increasing evidence of sex-differences in prostaglandin signaling and the importance of sex as a biological variable in data analysis. The preclinical literature identifies a sex difference in prostaglandin D2 synthase (PTGDS) expression where it is higher in female than in male rodents in the nervous system. We experimentally validated that PTGDS expression is higher in female human dorsal root ganglia (DRG) neurons recovered from organ donors. Our semi-systematic literature review reveals a need for continued inclusivity of both male and female animals in prostaglandins studies and data analysis separated by sex in preclinical and clinical studies. Our finding of sex-differences in neuronal PTGDS expression in humans exemplifies the need for a more comprehensive understanding of how the prostaglandin system functions in the DRG in rodents and humans.
Quartile coefficient of variation is more robust than CV for traits calculated as a ratio
Authors: Botta-Dukát Zoltán 
Published: March 2023

AbstractComparing within-species variations of traits can be used in testing ecological theories. In these comparisons, it is useful to remove the effect of the difference in mean trait values, therefore measures of relative variation, most often the coefficient of variation (CV), are used. The studied traits are often calculated as the ratio of the size or mass of two organs: e.g. specific leaf area (SLA) is the ratio of leaf size and leaf mass. Often the inverse of these ratios is also meaningful; for example, the inverse of SLA is often referred to as LMA (leaf mass per area). Relative variation of a trait and its inverse should not considerably differ. However, it is illustrated that using the coefficient of variation may result in differences that could influence the interpretation, especially if there are outlier trait values. The alternative way for estimating CV from the standard deviation of log-transformed data assuming log-normal distribution and Kirkwood’s geometric coefficient of variation free from this problem, but they proved to be sensitive to outlier values. Quartile coefficient of variation performed best in the tests: it gives the same value for a trait and its inverse and it is not sensitive to outliers.
NTSR1 glycosylation and MMP dependent cleavage generate three distinct forms of the protein
Authors: Libanje Fotine , Delille Raphael , Young Pamela A , Rolland Sylvie , Meyer-Losic Florence , Lewkowicz Elodie , Klinz Stephan 
Published: March 2023

AbstractNTSR1 abnormal expression by cancer cells makes it a strategic target for antitumoral therapies, such as compounds that use NTSR1 binding probes to deliver cytotoxic agents to tumor cells. Success of these therapies relies on NTSR1 protein availability and accessibility; therefore, understanding the protein’s biology is crucial. We studied NTSR1 protein in exogenously and endogenously expressing non-tumoral and tumoral cells. We found NTSR1 to be expressed as three distinct protein forms: the NTSR1-high form, a glycosylated protein; the NTSR1-low form, a N-terminally cleaved and de-glycosylated protein; and the NTSR1-LP protein with the MW size predicted by its NTSR1 amino acid sequence. We show that the NTSR1-high form is cleaved by MMPs to generate the NTSR1-low form, a process that is promoted by the Neurotensin (NTS) ligand. In addition, NTS induced the internalization of plasma membrane localized NTSR1 and degradation of NTSR1-low form via the proteasome. Importantly, we found NTSR1-low form to be the most abundant form in the tumoral cells and in PDAC Patient Derived Xenograft, demonstrating its physiopathological relevance. Altogether, our work provides important technical and experimental tools as well as new crucial insights into NTSR1 protein biology that are required to develop clinically relevant NTSR1 targeting anti-tumoral therapies.
Ring-opening metathesis polymerization of ester-functionalizedendo-tricyclo[4.2.2.02,5]deca-3,9-dienes and thermal properties of the resulting polymers
Authors: Asano Yuta , Aoi Hiromi , Ohtani Hajime , Matsuoka Shin-ichi , Suzuki Masato 
Published: March 2023

We investigated the ROMP of 1 and 2, and revealed the glass transition temperature and pyrolysis mechanisms of the resulting (co)polymers (with norbornene).
Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotonin
Authors: Fernando Shalinda J.A. , Wang Qian , Hay Debbie L. , Bathgate Ross A.D. , Shepherd Peter R. , Lee Kate L. 
Published: March 2023

Abstract
RXFP4 is a G protein–coupled receptor (GPCR) in the relaxin family. It has recently been recognised that this receptor and its cognate ligand INSL5 may have a role in the regulation of food intake, gut motility, and other functions relevant to metabolic health and disease. Recent data from reporter-mice showed co-location of Rxfp4 and serotonin (5-HT) in the lower gut. We used human single-cell RNA sequence data (scRNASeq) to show that RXFP4 is in a subset of gut enterochromaffin cells that produce 5-HT in humans. We also used RNAScope to show co-location of Rxfp4 mRNA and 5-HT in mouse colon, confirming prior findings. To understand whether RXFP4 might regulate serotonin production, we developed a cell model using Colo320, a human gut-derived immortalised cell line that produces and releases serotonin. Overexpression of RXFP4 in these cells resulted in a constitutive decrease in cAMP levels in both the basal state and in cells treated with forskolin. Treatment of cells with two RXFP4 agonists, INSL5 derived peptide INSL5-A13 and small molecule compound-4, further reduced cAMP levels. This was paralleled by a reduction in expression of mRNA for TPH1, the enzyme controlling the rate limiting step in the production of serotonin. Overexpression of RXFP4 also attenuated the cAMP-induced release of serotonin from Colo320 cells. Together this demonstrates that serotonin producing enterochromaffin cells are the major site of RXFP4 expression in the gut and that RXFP4 can have inhibitory functional impacts on cAMP production as well as TPH1 expression and serotonin release.
Asteroseismology of evolved stars to constrain the internal transport of angular momentum
Authors: Moyano F. D. , Eggenberger P. , Mosser B. , Spada F. 
Published: March 2023

Context. Asteroseismic measurements of the internal rotation rate in evolved stars pointed to a lack of angular momentum (AM) transport in stellar evolution models. Several physical processes in addition to hydrodynamical ones were proposed as candidates for the missing mechanism. Nonetheless, no current candidate can satisfy all the constraints provided by asteroseismology.
Aims. We revisit the role of a candidate process whose efficiency scales with the contrast between the rotation rate of the core and the surface. This process was proposed in previous works to be related to the azimuthal magneto-rotational instability.
Methods. We computed stellar evolution models of low- and intermediate-mass stars with the parametric formulation of AM transport proposed in previous works for this instability until the end of the core-helium burning for low- and intermediate-mass stars, and compare our results to the latest asteroseismic constraints available in the post-main sequence phase.
Results. Both hydrogen-shell-burning stars in the red-giant branch and core-helium-burning stars of low- and intermediate-mass in the mass range 1 M⊙ ≲ M ≲ 2.5 M⊙ can be simultaneously reproduced by this kind of parametrisation.
Conclusions. Given the current constraints from asteroseismology, the core rotation rate of post-main sequence stars seems to be well explained by a process whose efficiency is regulated by the internal degree of differential rotation in radiative zones.
A UNIONS view of the brightest central galaxies of candidate fossil groups
Authors: Chu A. , Durret F. , Ellien A. , Sarron F. , Adami C. , Márquez I. , Martinet N. , de Boer T. , Chambers K. C. , Cuillandre J.-C. , Gwyn S. , Magnier E. A. , McConnachie A. W. 
Published: March 2023

Context. The formation process of fossil groups (FGs) is still under debate, and, because of their relative rarity, large samples of such objects are still missing.
Aims. The aim of the present paper is to increase the sample of known FGs, to analyse the properties of their brightest group galaxies (BGGs), and to compare them with a control sample of non-FG BGGs.
Methods. We extracted a sample of 87 FG and 100 non-FG candidates from a large spectroscopic catalogue of haloes and galaxies. For all the objects with data available in UNIONS (initially the Canada France Imaging Survey, CFIS) in the u and r bands, and/or in an extra r-band processed to preserve all low-surface-brightness features (rLSB), we performed a 2D photometric fit of the BGG with GALFIT with one or two Sérsic components. We also analysed how the subtraction of the intracluster light (ICL) contribution modifies the BGG properties. From the SDSS spectra available for the BGGs of 65 FGs and 82 non-FGs, we extracted the properties of their stellar populations with Firefly. To complement our study, and in order to provide a detailed illustration of the possible origin of emission lines in the FG BGGs, involving the presence or absence of an AGN, we investigated the origin of the emission lines in a nearby FG that is dominated by the NGC 4104 galaxy.
Results. Morphologically, a single Sérsic profile can fit most objects in the u band, while two Sérsics are needed in the r and rLSB bands, both for FGs and non-FGs. Non-FG BGGs cover a larger range of Sérsic index n. FG BGGs follow the Kormendy relation (mean surface brightness versus effective radius) previously derived for almost 1000 brightest cluster galaxies (BCGs), while the majority of non-FGs BGGs are located below this relation, with fainter mean surface brightnesses. This suggests that FG BGGs have evolved similarly to BCGs, and non-FG BGGs have evolved differently from both FG BGGs and BCGs. All the above properties can be strongly modified by the subtraction of the ICL contribution. Based on spectral fitting, the stellar populations of FG and non-FG BGGs do not differ significantly.
Conclusions. FG and non-FG BGGs differ from one another in terms of their morphological properties and Kormendy relation, suggesting they have had different formation histories. However, it is not possible to trace differences in their stellar populations or in their large-scale distributions.
Das digitale Unbewusste und die
Balint-Arbeit
Authors: Castigliego Guiliano 
Published: March 2023

ZusammenfassungUnter dem Aspekt des Sehens und Gesehenwerdens werden die im Bereich der neuen
Medien entstehende psychischen Prozesse bei den Nutzern intrasubjektiv und
interaktionell thematisiert. Dies erfolgt unter Hinweis auf Freud und den
Begriffen des Unbewussten und des Traumverständnisses. Auch für
das Digitale gilt: Es gibt in diesem System keine Negation, keinen Zweifel,
keine Grade von Sicherheit. Der Begriff eines psychodynamischen digitalen
Unbewussten wird eingeführt und erläutert. Die Konsequenzen
für die Balintgruppe werden beschrieben mit der Möglichkeit
diese auch als sozialen Übergangsraum zu verstehen.
Grundwerte: Zusammenhalt und Flexibilität: von Kleingruppen
bis zur IBF
Authors: Parée Michèle 
Published: March 2023

ZusammenfassungGibt es noch einen Zusammenhang zwischen der Balint-Arbeit in den
fünfziger Jahren und dem, was wir heute tun? Die Teilnahme an einer
Balintgruppe braucht Zeit, hilft es den Betreuern wirklich? Lohnen sich
Balintgruppen noch? Die Medizin hat sich verändert, es gibt mehr
wissenschaftliche Erkenntnisse und die Technologie hat sich weiterentwickelt,
die Hausärzte sind der Allgemeinmedizin gewichen, ist es nicht besser,
einen akademischen Kurs zu besuchen, um ihr Wissen zu erweitern? Und was ist mit
einer so „großen Gruppe“ wie der IBF? Wie können
wir eine effektive Zusammenarbeit fortsetzen? Wir haben Mindestkriterien, reicht
das? Teilen wir die gleichen Grundwerte? Was sind sie? Ist die IBF-Einheit ein
Mythos? Welchen Stellenwert hat Klatsch in unserem Bereich? Die Pandemie und die
steigenden Mitgliederzahlen könnten uns in eine Krise führen.
Wie können wir das durchstehen, ohne uns
„persönlich“ zu treffen?
Effect of Tinnitus on Sleep Quality and Insomnia
Authors: Gallo Katherine Eloise Bornancin , Corrêa Camila de Castro , Gonçalves Claudia Giglio de Oliveira , Correia Baran Jordana Batista , Marques Jair Mendes , Zeigelboim Bianca Simone , José Maria Renata 
Published: March 2023

Abstract
Introduction Tinnitus is a conscious perception of a sound resulting from abnormal activity within the nervous system. A relevant percentage of tinnitus patients report symptoms severe enough to significantly affect quality of life, including sleep disorders.
Objective To analyze the sleep quality, insomnia, daytime sleepiness, and risk of obstructive sleep apnea (OSA) in participants with tinnitus.
Methods The sample comprised 18 adults and older adults aged between 18 and 85 years old (mean age = 58.7 ± 17.5 years old), females and males, with complaint of continuous tinnitus for > 1 month. The instruments used were the Tinnitus Handicap Inventory (THI) questionnaire, the Insomnia Severity Index, the Pittsburgh Sleep Quality Index, the Epworth Sleepiness Scale, and the STOP-Bang questionnaire.
Results By means of the THI questionnaire, the tinnitus severity degree reported by most participants was mild (27.8%) and moderate (27.8%), having a positive (r = 0.582) and significant (0.011) correlation to sleep quality, measured by means of the Pittsburgh questionnaire. There was a positive correlation between the Insomnia Severity Index and tinnitus handicap (r = 0.499; p = 0.035). A total of 72.2% of the participants self-assessed their sleep quality as poor, in addition to moderate insomnia (27.8%), although there is low risk of OSA (66.7%), without complaints of excessive daytime sleepiness (72.2%).
Conclusion Subjects with tinnitus complaint self-rated their sleep quality as poor. Moreover, the higher the reported tinnitus handicap, the greater the symptoms of insomnia. There was no influence of tinnitus in relation to daytime sleepiness and no relationship between the severity of tinnitus and the risk of OSA.
IScore, a useful prognostic tool for patients with acute ischemic stroke treated with intravenous thrombolysis: a validation study
Authors: Scavasine Valéria Cristina , Costa Rebeca Teixeira , Zétola Viviane de Hiroki Flumignan , Lange Marcos Christiano 
Published: March 2023

Abstract
Background Stroke is one of the major causes of disability and mortality worldwide. Up to 30% of individuals who experience stroke die within 30 days, and more than 50% of those who survive will have some degree of disability. There are some predetermining factors based on admission data that could be used to objectively assess the odds of poor outcomes, including the Ischemic Stroke Predictive Risk Score (IScore).
Objective To analyze and validate the IScore in patients undergoing intravenous thrombolysis for stroke and compare the results of this predictor with actual death and disability outcomes.
Methods In a retrospective study, data were collected from a database housed at the Stroke Unit of the Teaching Hospital of Universidade Federal do Paraná, Southern Brazil. The IScore was applied to admission data from 239 patients, and the results were compared with actual outcomes (death and disability) within 30 days and 1 year after the stroke event. Data analysis was performed using an analysis of the receiver operating characteristic (ROC) curve to determine the sensitivity and specificity of the IScore in the study population.
Results The IScore demonstrated moderate sensitivity and high specificity in patients with stroke who underwent thrombolysis when evaluated after 30 days and 1 year of the event.
Conclusions The IScore can be applied to in stroke patients undergoing thrombolysis; therefore, it may be used as an objective prognostic tool to guide clinical decision-making. Understanding the prognosis of patients in the acute phase can assist clinicians in making the best therapeutic decisions and enable better end-of-life care.
Neurological symptoms and comorbidity profile of hospitalized patients with COVID-19
Authors: Cremaschi Renata Carvalho , Bahi Carla Alessandra Scorza , Paola Angelo Amato Vincenzo de , Arakaki Jaquelina Sonoe Ota , Ferreira Paulo Roberto Abrão , Bellei Nancy Cristina Junqueira , Borges Vanderci , Coelho Fernando Morgadinho Santos 
Published: March 2023

Abstract
Background The neurological manifestations in COVID-19 adversely impact acute illness and post-disease quality of life. Limited data exist regarding the association of neurological symptoms and comorbid individuals.
Objective To assess neurological symptoms in hospitalized patients with acute COVID-19 and multicomorbidities.
Methods Between June 2020 and July 2020, inpatients aged 18 or older, with laboratory-confirmed COVID-19, admitted to the Hospital São Paulo (Federal University of São Paulo), a tertiary referral center for high complexity cases, were questioned about neurological symptoms. The Composite Autonomic Symptom Score 31 (COMPASS-31) questionnaire was used. The data were analyzed as a whole and whether subjective olfactory dysfunction was present or not.
Results The mean age of the sample was 55 ± 15.12 years, and 58 patients were male. The neurological symptoms were mostly xerostomia (71%), ageusia/hypogeusia (50%), orthostatic intolerance (49%), anosmia/hyposmia (44%), myalgia (31%), dizziness (24%), xerophthalmia (20%), impaired consciousness (18%), and headache (16%). Furthermore, 91% of the patients had a premorbidity. The 44 patients with subjective olfactory dysfunction were more likely to have hypertension, diabetes, weakness, shortness of breath, ageusia/hypogeusia, dizziness, orthostatic intolerance, and xerophthalmia. The COMPASS-31 score was higher than that of previously published controls (14.85 ± 12.06 vs. 8.9 ± 8.7). The frequency of orthostatic intolerance was 49% in sample and 63.6% in those with subjective olfactory dysfunction (2.9-fold higher risk compared to those without).
Conclusion A total of 80% of inpatients with multimorbidity and acute COVID-19 had neurological symptoms. Chemical sense and autonomic symptoms stood out. Orthostatic intolerance occurred in around two-thirds of the patients with anosmia/hyposmia. Hypertension and diabetes were common, mainly in those with anosmia/hyposmia.
Blood flow restricted walking alters gait kinematics
Authors: Walden Thomas P. , Fairchild Timothy , Girard Olivier , Peiffer Jeremiah J. , Jonson Andrew M. , Dempsey Alasdair R. 
Published: March 2023

ABSTRACTThis study investigated the impact of blood flow restriction (BFR) during treadmill walking on gait kinematics. Twenty‐one participants completed one familiarisation and four experimental sessions, including two walking speeds (moderate [5.0 ± 0.3km·h−1] and fast [6.4 ± 0.4km·h−1]) and two occlusion conditions (BFR [60% of arterial occlusion pressure] and unrestricted). For each exercise intensity, the BFR session was performed first. Participants were instructed to walk as long as possible, with sessions capped at 20 min. Unrestricted sessions were time‐matched, and the order of exercise intensity was randomised. Kinematics were collected over 10s every minute using retro‐reflective markers affixed to specific body landmarks. Ratings of perceived exertion and discomfort were collected every two minutes. Blood samples were collected from the fingertip pre‐exercise and the finger and toe post‐exercise, and were analysed for lactate, electrolytes, and markers of cell‐membrane damage. During the BFR sessions the cuffs remained inflated while the blood samples were collected. Fast‐walk BFR sessions exhibited higher anterior trunk flexion (p = 0.001) and knee flexion during stance (p = 0.001) compared to all other sessions. Step width was increased during BFR sessions (p = 0.001), but no difference in step length (p = 0.300) or cadence (p = 0.922) were observed. The time required to elicit change in anterior trunk flexion and plantar‐flexion angle at toe‐off was shorter during BFR sessions (p = 0.024). The BFR sessions elicited the highest ratings of perceived exertion and discomfort, as well as blood lactate concentration (p ≤ 0.001). Application of BFR during moderate and fast treadmill walking modifies gait kinematics and exacerbates exercise‐related sensations as well as blood lactate concentration.