bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production, characterization and techno-economic evaluation of Aspergillus fusant l-asparaginase
Authors: Asitok Atim , Ekpenyong Maurice , Amenaghawon Andrew , Akwagiobe Ernest , Asuquo Marcus , Rao Anitha , Ubi David , Iheanacho Juliet , Etiosa Joyce , Antai Agnes , Essien Joseph , Antai Sylvester 
Published: January 2023

AbstractProtoplast fusion is one of the most reliable methods of introducing desirable traits into industrially-promising fungal strains. It harnesses the entire genomic repertoire of fusing microorganisms by routing the natural barrier and genetic incompatibility between them. In the present study, the axenic culture of a thermo-halotolerant strain of Aspergillus candidus (Asp-C) produced an anti-leukemic l-asparaginase (l-ASNase) while a xylan-degrading strain of Aspergillus sydowii (Asp-S) produced the acrylamide-reduction type. Protoplast fusion of the wild strains generated Fusant-06 with improved anti-leukemic and acrylamide reduction potentials. Submerged fed-batch fermentation was preferred to batch and continuous modes on the basis of impressive techno-economics. Fusant-06 l-ASNase was purified by PEG/Na+ citrate aqueous two-phase system (ATPS) to 146.21-fold and global sensitivity analysis report revealed polymer molecular weight and citrate concentration as major determinants of yield and purification factor, respectively. The enzyme was characterized by molecular weight, amino acid profile, activity and stability to chemical agents. Michaelis–Menten kinetics, evaluated under optimum conditions gave Km, Vmax, Kcat, and Kcat/Km as 6.67 × 10–5 M, 1666.67 µmolmin−1 mg−1 protein, 3.88 × 104 min−1 and 5.81 × 108 M−1.min−1 respectively. In-vitro cytotoxicity of HL-60 cell lines by Fusant-06 l-ASNase improved significantly from their respective wild strains. Stability of Fusant-06 l-ASNase over a wide range of pH, temperature and NaCl concentration, coupled with its micromolar Km value, confers commercial and therapeutic value on the product. Free-radical scavenging and acrylamide reduction activities were intermediate and the conferred thermo-halo-stability could be exploited for sustainable clinical and food industry applications.
History of the Development of Analytical Models of Cracking of Restrained Walls on a Given Edge Since 1968
Authors: Zych Mariusz 
Published: January 2023

AbstractThis research paper presents and comments on analytical models for calculating the widths of cracks formed as a result of imposed deformations generating tensile stresses in reinforced concrete base-restrained members. This issue regarding the mechanics of concrete structures has been presented on the basis of calculation models since 1968. In accordance with the current regulations of the European standard, the mechanics of the cracking of base-restrained members have been presented in a very simplified way, which was justified by a limited number of research studies performed on such members as well as in a few subject publications. The main purpose of this work was to present especially those models that had the greatest practical significance within a specific period of time or formed the basis for further studies of other authors. In addition, future trends in the development of computational tools are presented. The chronologically presented development of design ideas, which takes into account varying degrees of advancement of the mechanics of cracking due to the distinctly different design consequences, is a valuable source of information and an inspiration for subsequent researchers. In the second part of the paper, a few of the most important issues connected with the calculation of the crack width in base-restrained walls are presented. It is shown that currently, on the basis of the up-to-date knowledge, there are possibilities to create more complementary standard guidelines, which is already taking place in the case of European guidelines.
Bacteroides-derived isovaleric acid enhances mucosal immunity by facilitating intestinal IgA response in broilers
Authors: Wang Xinkai , Hu Yifan , Zhu Xiaoyan , Cai Liyuan , Farooq Muhammad Zahid , Yan Xianghua 
Published: January 2023

Abstract
Background
The interaction between nutrition and immunity plays a vital role in nutrient digestion, absorption, and metabolism during poultry production. Recent studies showed that the gut microbiota contributes to the development of intestinal mucosal immunity. However, the mechanisms by which gut microbes regulate this process remain unclear.

Methods
We compared the intestinal mucosal immunity and gut microbiota of Arbor Acre broilers (AA (lower mucosal immunity) and Chinese native Wuliang Mountain Black-bone chickens (WLMB) (higher mucosal immunity) using 16S rDNA sequencing, transcriptomic analysis, and immunoglobulin A (IgA) antibody repertoire sequencing. We then combined 16S rDNA sequencing with transcriptomics to identify the key microbes and found that they were positively correlated with IgA production. Next, we transplanted candidate microbes into 1-day-old broiler to explore their role in intestinal mucosal immunity. Finally, we verified the function of candidate microbial metabolites in regulating the immune function of macrophages and the intestinal-epithelial cells (IECs) using in vitro experiments.

Results
WLMB performs stronger mucosal immunity than AA, including higher IgA levels, more diverse IgA antibody repertoire, and higher bacterial affinity. Bacteroides was identified as the key microbes related to the intestinal IgA response. Bacteroides transplantation could increase IgA concentration in the duodenal contents by enhancing the expression of IgA, polymeric immunoglobin receptor (PIgR), B cell-activating factor of the TNF family (BAFF), and activation-induced cytidine deaminase (AID) in the duodenum. Additionally, Bacteroides-derived isovaleric acid promoted M2 macrophage polarization of macrophage via mTOR/PPAR-γ/STAT3 signaling pathways and regulated the immunologic function of IECs to produce cytokines, including interleukin (IL)-10, IL-4, BAFF, and transforming growth factor-beta (TGF-β), thus promoting IgA production in B cells by facilitating AID expression.

Conclusion
Our study revealed that Bacteroides modulate the intestinal IgA response and maintain gut health in broilers. Bacteroides may be a promising alternative as an immunomodulatory microbial agent for developing next-generation probiotics for broiler production.
Psychological challenges and related factors of ordinary residents after “7.20” heavy rainstorm disaster in Zhengzhou: a cross-sectional survey and study
Authors: Wang Zhifeng , Jiang Bing , Wang Xingtong , Wang Dongxu , Xue Haihong 
Published: January 2023

Abstract
Background
In 2021, a once-in-a-century heavy rainstorm suddenly attacked Zhengzhou, an important inland city in northern China. However, there have been no studies on the psychological health of disaster-stricken residents. This study is the first to comprehensively report on the mental health status and related factors of local ordinary residents after the heavy rainstorm.

Objective
The purpose of this study is to investigate the mental health status and related influencing factors of local ordinary residents after the flood disaster, and to provide reference for government departments to formulate disaster psychological intervention countermeasures based on evidence-driven strategies.

Methods
The snowball sampling technique was used in this study, and measurement tools of Rainstorm Exposure Questionnaire, Subjective Perception of Rainstorm, Post-Traumatic Stress Disorder Checklist-Civilian version (PCL-C), Depression, Anxiety and Stress Scale-21 (DAS-21) and Chinese version of Social Support Rating Scale (SSRS) were used to evaluate the rainstorm exposure, subjective perception of the rainstorm, psychological symptoms and social support of the disaster-stricken residents within a week after the rainstorm. Logistic regression analysis was used to examine the psychological status and related factors of local residents after the rainstorm disaster.

Results
A total of 469 valid samples were obtained in this study. All the subjects were in the disaster area and experienced the rainstorm personally, with normal intelligence. The statistical results showed that 25.37% people had experienced at least three rainstorm-related stresses, nearly 20.26% people had post-traumatic stress disorder (PTSD) symptoms, and 39.3%, 53.92% and 65.83% people had depression, anxiety and stress symptoms, respectively. Multivariable logistic regression analyses indicated that female (all p < 0.05), the divorced, agricultural workers/farmers (all p < 0.05), students (all p < 0.05), people experiencing at least three rainstorm-related stresses (p < 0.05 or p < 0.01), people with lower satisfaction at the social flood fighting measures (p < 0.05 or p < 0.01) and people with low social support (p < 0.05 or p < 0.01) were all independent risk factors for poor psychological health, and college education or above (p < 0.05 or p < 0.01), the lower degree of worrying about themselves (all p < 0.01), family members (all p < 0.01) and family property (all p < 0.01) were all related to higher psychological health among flood survivors after the disaster.

Conclusions
Rainstorm could cause local residents to have various degrees of psychological symptoms. This study identified factors associated with the psychological health of disaster-stricken residents, which could be used to develop psychological interventions in improving psychological health of local residents.
From pilot knowledge via integrated reservoir characterization to utilization perspectives of deep geothermal reservoirs: the 3D model of Groß Schönebeck (North German Basin)
Authors: Norden Ben , Bauer Klaus , Krawczyk Charlotte M. 
Published: January 2023

AbstractThe Groß Schönebeck site in the North German Basin serves as research platform to study the geothermal potential of deeply buried Permian reservoir rocks and the technical feasibility of heat extraction. The structural setting of the site was investigated in more detail by a newly acquired 3D-seismic survey to improve the former conceptual model that was based on several old 2D seismic lines. The new data allow a revision of the geological interpretation, enabling the setup of a new reservoir model and providing base information for a possible further site development of Permo-Carboniferous targets. The 3D seismic allows for the first time a consistent geological interpretation and model parameterization of the well-studied geothermal site. Main reflector horizons and the corresponding stratigraphic units were mapped and the structural pattern of the subsurface presented in the 8 km × 8 km × 4 km large seismic volume. Attribute analysis revealed some fracture and fault patterns in the upper Zechstein and post-Permian units, while formerly hypothesized large offset faults are not present in the Rotliegend reservoir. However, a well-established graben-like structure at the top of the Zechstein succession is most likely related to broken anhydritic brittle intra-salt layers of some meter of thickness. Most reflectors above the salt show a rather undisturbed pattern. The main reservoir sandstone of the Dethlingen Formation (Rotliegend) was mapped and characterized. The base of the underlying Permo-Carboniferous volcanic rock sequence and hence its thickness could not be depicted reliably from the geophysical data. Based on the seismic data and the available reconnaissance drilling, logging, and laboratory data of the Groß Schönebeck research site, the thickness and distribution of the sedimentary Rotliegend (with emphasis of the sandy reservoir section) and of the volcanic rock sequence was modelled and stochastically parameterized with petrophysical properties guided by seismic facies pattern correlation, providing a more realistic reservoir description. Properties include total and effective porosity, permeability, bulk density, thermal conductivity, thermal diffusivity, and specific heat capacity. The data and interpretation constitute the basis for a better understanding of the thermo and hydromechanical processes at the site and for future measures. Further site development could include a deepening of one well to provide evidence on the volcanic rock sequence and consider deviated wells into favourable zones and the design of a fracture-dominated utilization approach.
Inclusion in practice: a systematic review of diversity-focused STEM programming in the United States
Authors: Palid Olivia , Cashdollar Sarah , Deangelo Sarah , Chu Chu , Bates Meg 
Published: January 2023

AbstractColleges across the United States have shown a commitment to advancing diversity in the STEM fields by creating programs aimed at improving outcomes of women and/or racially and ethnically minoritized students. However, most existing literature focuses on the successes of singular college programs rather than comparing these STEM interventions across the higher education landscape. This systematic review investigates the literature on diversity-focused “STEM intervention programs” (SIPs) at the postsecondary level. We categorize key features of these programs and their outcomes, and we look at which program components have the most empirical support. We examine 82 articles that reported on SIPs with disaggregated outcomes, coding each initiative’s features and outcomes. Across these articles, we found six common program components, with most programs including more than one component, and five common program outcomes. Just 53 articles tested differences in outcomes of participants relative to a comparison group. This subset of research found support for the effectiveness of all coded components for improving student outcomes, though studies of multi-component programs did not parse the relative contributions of each component. Based on these findings, we conclude multi-component interventions that create a welcoming environment and focus on the successes of minoritized students help redress existing institutional shortcomings and are a promising step towards diversity, equity, and inclusion in STEM. However, more rigorous quantitative studies are needed to empirically assess the effectiveness of individual SIP program components.
Ventilation during continuous compressions or at 30:2 compression-to-ventilation ratio results in similar arterial oxygen and carbon dioxide levels in an experimental model of prolonged cardiac arrest
Authors: Kopra Jukka , Litonius Erik , Pekkarinen Pirkka T. , Laitinen Merja , Heinonen Juho A. , Fontanelli Luca , Mäkiaho Tomi P. , Skrifvars Markus B. 
Published: January 2023

Abstract
Background
In refractory out-of-hospital cardiac arrest, transportation to hospital with continuous chest compressions (CCC) from a chest compression device and ventilation with 100% oxygen through an advanced airway is common practice. Despite this, many patients are hypoxic and hypercapnic on arrival, possibly related to suboptimal ventilation due to the counterpressure caused by the CCC. We hypothesized that a compression/ventilation ratio of 30:2 would provide better ventilation and gas exchange compared to asynchronous CCC during prolonged experimental cardiopulmonary resuscitation (CPR).

Methods
We randomized 30 anaesthetized domestic swine (weight approximately 50 kg) with electrically induced ventricular fibrillation to the CCC or 30:2 group and bag-valve ventilation with a fraction of inspired oxygen (FiO2) of 100%. We started CPR after a 5-min no-flow period and continued until 40 min from the induction of ventricular fibrillation. Chest compressions were performed with a Stryker Medical LUCAS® 2 mechanical chest compression device. We collected arterial blood gas samples every 5 min during the CPR, measured ventilation distribution during the CPR using electrical impedance tomography (EIT) and analysed post-mortem computed tomography (CT) scans for differences in lung aeration status.

Results
The median (interquartile range [IQR]) partial pressure of oxygen (PaO2) at 30 min was 110 (52–117) mmHg for the 30:2 group and 70 (40–171) mmHg for the CCC group. The median (IQR) partial pressure of carbon dioxide (PaCO2) at 30 min was 70 (45–85) mmHg for the 30:2 group and 68 (42–84) mmHg for the CCC group. No statistically significant differences between the groups in PaO2 (p = 0.40), PaCO2 (p = 0.79), lactate (p = 0.37), mean arterial pressure (MAP) (p = 0.47) or EtCO2 (p = 0.19) analysed with a linear mixed model were found. We found a deteriorating trend in PaO2, EtCO2 and MAP and rising PaCO2 and lactate levels through the intervention. There were no differences between the groups in the distribution of ventilation in the EIT data or the post-mortem CT findings.

Conclusions
The 30:2 and CCC protocols resulted in similar gas exchange and lung pathology in an experimental prolonged mechanical CPR model.
COVID-19 and tourism sector stock price in Spain: medium-term relationship through dynamic regression models
Authors: Carrillo-Hidalgo Isabel , Pulido-Fernández Juan Ignacio , Durán-Román José Luis , Casado-Montilla Jairo 
Published: January 2023

AbstractThe global pandemic, coronavirus disease 2019 (COVID-19), has significantly affected tourism, especially in Spain, as it was among the first countries to be affected by the pandemic and is among the world’s biggest tourist destinations. Stock market values are responding to the evolution of the pandemic, especially in the case of tourist companies. Therefore, being able to quantify this relationship allows us to predict the effect of the pandemic on shares in the tourism sector, thereby improving the response to the crisis by policymakers and investors. Accordingly, a dynamic regression model was developed to predict the behavior of shares in the Spanish tourism sector according to the evolution of the COVID-19 pandemic in the medium term. It has been confirmed that both the number of deaths and cases are good predictors of abnormal stock prices in the tourism sector.
Isolated surgical vs. transcatheter aortic valve replacement: a propensity score analysis
Authors: Almedimigh Abdulmalik A. , Albabtain Monirah A. , Alfayez Latifa A. , Alsubaie Faisal F. , Almoghairi Abdulrahman , Alotaiby Mohammad , Alkhushail Abdullah , Ismail Huda , Pragliola Claudio , Adam Adam I. , Arafat Amr A. 
Published: January 2023

Abstract
Background
The debate about the optimal approach for aortic valve replacement continues. We compared the hospital and long-term outcomes (survival, aortic valve reintervention, heart failure readmissions, and stroke) between transcatheter vs. surgical (TAVR vs. SAVR) aortic valve replacement. The study included 789 patients; 293 had isolated SAVR, and 496 had isolated TAVR. Patients with concomitant procedures were excluded. Propensity score matching identified 53 matched pairs.

Results
Patients who had TAVR were significantly older (P ˂ 0.001) and had significantly higher EuroSCORE II (P ˂ 0.001), NYHA class (P ˂ 0.001), and more prevalence of diabetes mellitus (P ˂ 0.001), hypertension (P ˂ 0.001), chronic lung disease (P = 0.001), recent myocardial infarction (P = 0.002), and heart failure (P ˂ 0.001), stroke (P = 0.02), atrial fibrillation (P = 0.004), and previous percutaneous coronary interventions (P ˂ 0.001) than SAVR patients. In the matched cohort, atrial fibrillation occurred more frequently after SAVR (P = 0.01), and hospital stay was significantly longer in SAVR patients (P ˂ 0.001). There were no differences in hospital mortality between groups (P ˃ 0.99). Survival at 1, 3, and 5 years was 97%, 95%, and 94% for SAVR and 91%, 79%, and 58% for TAVR patients. Survival was lower in TAVR patients before matching (P ˂ 0.001) and after matching (P = 0.045). Freedom from the composite endpoint of stroke, aortic valve reintervention, and heart failure readmission at 1, 3, and 5 years was 98.9%, 96%, and 94% for SAVR and 94%, 86%, and 75% for TAVR. The composite endpoint was significantly higher in the TAVR group than in SVR before matching (P ˂ 0.001), while there was no difference after matching (P = 0.07). There was no significant difference in the change in ejection fraction between groups (β: −0.88 (95% CI: −2.20–0.43), P = 0.19), and the reduction of the aortic valve peak gradient was significantly higher with TAVR (β: −7.80 (95% CI: −10.70 to −4.91); P ˂ 0.001).

Conclusions
TAVR could reduce postoperative atrial fibrillation and hospital stay. SAVR could have long-term survival benefits over TAVR with comparable long-term stroke, heart failure readmission, and aortic valve reinterventions between SAVR and TAVR.
Association between pelvic inclination and balance in children with spastic diplegia
Authors: Allah Naglaa Ezzat Abd , Kamal Hebatallah Mohamed , El-Nabie Walaa Abd El-Hakiem Abd 
Published: January 2023

Abstract
Background
Children with spastic diplegia experience gait abnormalities and problems caused by deficits in balance, motor control, and spasticity. Abnormal pelvic inclination is common in patients with diplegia which may result in poor pelvic balance.

Purpose
This study was conducted to investigate the relation between pelvic inclination and standing balance in children with spastic diplegia.

Subjects and methods
Thirty children with spastic diplegic cerebral palsy from both sexes, aged from 5 to 14 years participated in this study. Their degree of spasticity ranged from 1 to 1+ according to Modified Ashworth Scale and they were on level I or II on Gross Motor Function Classification System. Pelvic inclination angle was measured by using the formetric instrumentation system during standing position while standing balance was assessed by the Biodex Balance System.

Results
There was a statistically significant relation between pelvic inclination and the overall, anteroposterior, and mediolateral stability indices of standing balance (P < 0.05).

Conclusion
The obtained results suggested that there was significant correlation between balance and pelvic inclination in children with diplegic cerebral palsy.