bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantification of Phenolic Component by LC‐HESI‐MS/MS and Evaluation of Antioxidant Activities of Crocus Ancyrensis (Ankara Çiğdemi) Extracts Obtained with Different Solvents
Authors: Kayir Ömer , Doğan Hacer , Alver Erol , Bilici İbrahim 
Published: February 2023

AbstractIn this study, Crocus ancyrensis was extracted from different parts of the plants with various solvents and their antioxidant activities and phenolic contents were investigated in detail for the first time. The highest amount of total phenolic substance in all parts of the plant was determined in the stigmaless flower. In the DPPH and FRAP methods, the highest antioxidant activity was obtained from the water extraction from the plant. Rutin is the highest detected by LC MS/MS. Stigmaless flower extract in all solvents is attributed to the component that contributes the most to antioxidant capacity. p‐hydroxy benzoic acid was detected as the highest phenolic component after rutin. When the antioxidant activity results were examined, it was determined that the highest activity was in the water extract. As a result, it is evaluated that rutin and p‐hydroxybenzoic acid in the plant contribute to the antioxidant capacity.
Rapid Biocatalytic Synthesis of Aromatic Acid CoA Thioesters by Using Microbial Aromatic Acid CoA Ligases
Authors: Chaudhury Debayan , Torkelson Ella R. , Meyers Kaya A. , Acheson Justin F. , Landucci Leta , Pu Yucen , Sun Zimou , Tonelli Marco , Bingman Craig A. , Smith Rebecca A. , Karlen Steven D. , Mansfield Shawn D. , Ralph John , Fox Brian G. 
Published: February 2023

AbstractChemically labile ester linkages can be introduced into lignin by incorporation of monolignol conjugates, which are synthesized in planta by acyltransferases that use a coenzyme A (CoA) thioester donor and a nucleophilic monolignol alcohol acceptor. The presence of these esters facilitates processing and aids in the valorization of renewable biomass feedstocks. However, the effectiveness of this strategy is potentially limited by the low steady‐state levels of aromatic acid thioester donors in plants. As part of an effort to overcome this, aromatic acid CoA ligases involved in microbial aromatic degradation were identified and screened against a broad panel of substituted cinnamic and benzoic acids involved in plant lignification. Functional fingerprinting of this ligase library identified four robust, highly active enzymes capable of facile, rapid, and high‐yield synthesis of aromatic acid CoA thioesters under mild aqueous reaction conditions mimicking in planta activity.
Lysosomes Targeting pH Activable Imaging‐Guided Photodynamic Agents
Authors: Figliola Carlotta , Anton Halina , Sutter Christophe , Chériaux Camille , Sutter Alexandra , Mazan Valérie , Elhabiri Mourad , Didier Pascal , Jacquemin Denis , Ulrich Gilles 
Published: February 2023

AbstractPhotodynamic therapy (PDT) is a photochemistry‐based medical treatment combining light at a specific wavelength and a photosensitizer (PS) in the presence of oxygen. Application of PDT as a conventional treatment is limited and clearly the approval in clinics of new PS is challenging. The selective accumulation of the PS in the targeted malignant cells is of paramount importance to reduce the side effects that are typical of the current worldwide approved PS. Here we report a new series of aniline‐ and iodine‐substituted BODIPY derivatives (1–3) as promising lysosome‐targeting and pH‐responsive theranostic PS, which displayed a significant in vitro light‐induced cytotoxicity, efficient imaging properties and low dark toxicity (for 2 and 3). These compounds were obtained in few reproducible synthetic steps and good yields. Spectroscopic and electrochemical measurements along with computational calculations confirmed the quenching of the emissive properties of the PS, while both fluorescence and 1O2 emission were obtained only under acidic conditions inducing amine protonation. The pKa values and pH‐dependent emissive properties of 1–3 being established, their cellular uptake and activation in the lysosomal vesicles (pH≈4‐5) were confirmed by their co‐localization with the commercial LysoTracker deep red and light‐induced cytotoxicity (IC50 between 0.16 and 0.06 μM) against HeLa cancer cells.
Impact of frailty on outcomes in acute ST‐elevated myocardial infarctions undergoing percutaneous coronary intervention
Authors: Heaton Joseph , Singh Sohrab , Nanavaty Dhairya , Okoh Alexis K. , Kesanakurthy Srinivas , Tayal Rajiv 
Published: February 2023

AbstractAimsWe analyzed the impact of frailty on readmission rates for ST‐elevated myocardial infarctions (STEMIs) and the utilization of percutaneous coronary intervention (PCI) in STEMI admissions.Methods and ResultsThe 2016−2019 Nationwide Readmission Database was analyzed for patients admitted with an acute STEMI. Patients were categorized by frailty risk and analyzed for 30‐day readmission risk after acute STEMIs, PCI utilization and outcomes, and healthcare resource utilization.Qualifying index admissions were found in 584,918 visits. Low risk frailty was noted in 78.20%, intermediate risk in 20.67%, and high risk in 1.14% of admissions. Thirty‐day readmissions occurred in 7.74% of index admissions, increasing with frailty (p < 0.001). Readmission risk increased with frailty, 1.37 times with intermediate and 1.21 times with high‐risk frailty.PCI was performed in 86.40% of low‐risk, 66.03% of intermediate‐risk, and 58.90% of high‐risk patients (p < 0.001). Intermediate patients were 55.02% less likely and high‐risk patients were 61.26% less likely to undergo PCI (p < 0.001). Length of stay means for index admissions were 2.96, 7.83, and 16.32 days for low, intermediate, and high‐risk groups. Intermediate and high‐risk frailty had longer length of stay, higher total cost, and were more likely to be discharged to a skilled facility (p < 0.001).ConclusionAmong adult, all‐payer inpatient visits, frailty discerned by the hospital frailty risk score was associated with increased readmissions, increased healthcare resource utilization, and lower PCI administration.
Complex primary percutaneous coronary intervention with ultrathin‐strut biodegradable versus thin‐strut durable polymer drug‐eluting stents in patients with ST‐segment elevation myocardial infarction: A subgroup analysis from the BIOSTEMI randomized trial
Authors: Iglesias Juan F. , Muller Olivier , Losdat Sylvain , Roffi Marco , Kurz David J. , Weilenmann Daniel , Kaiser Christoph , Heg Dik , Windecker Stephan , Pilgrim Thomas 
Published: February 2023

AbstractBackgroundUltrathin‐strut biodegradable polymer sirolimus‐eluting stents (BP‐SES) are superior to thin‐strut durable polymer everolimus‐eluting stents (DP‐EES) with respect to target lesion failure (TLF) at 2 years among patients with ST‐segment elevation myocardial infarction (STEMI). We sought to determine the impact of primary percutaneous coronary intervention (pPCI) complexity on long‐term clinical outcomes with BP‐SES versus DP‐EES in STEMI patients.MethodsWe performed a post hoc subgroup analysis from the BIOSTEMI (NCT02579031) randomized trial, which included individual data from 407 STEMI patients enrolled in the BIOSCIENCE trial (NCT01443104). STEMI patients were randomly assigned to treatment with ultrathin‐strut BP‐SES or thin‐strut DP‐EES, and further categorized into those undergoing complex versus noncomplex pPCI. Complex pPCI was defined by the presence of ≥1 of the following criteria: 3 vessel treatment, ≥3 stents implanted, ≥3 lesions treated, bifurcation lesion with ≥2 stents implanted, total stent length ≥60 mm, and/or chronic total occlusion treatment. The primary endpoint was TLF, a composite of cardiac death, target‐vessel myocardial reinfarction, or clinically indicated target lesion revascularization, within 2 years.ResultsAmong a total of 1707 STEMI patients, 421 (24.7%) underwent complex pPCI. Baseline characteristics were similar between groups. At 2 years, TLF occurred in 14 patients (7.1%) treated with BP‐SES and 25 patients (11.6%) treated with DP‐EES (hazard ratio [HR]: 0.62; 95% confidence interval [CI]: 0.32–1.19; p = 0.15) in the complex pPCI group, and in 28 patients (4.4%) treated with BP‐SES and 49 patients (8.2%) treated with DP‐EES (HR: 0.54; 95% CI: 0.34–0.86; p = 0.008; p for interaction = 0.74) in the noncomplex pPCI group. Individual TLF components and stent thrombosis rates did not significantly differ between groups.ConclusionIn a post hoc subgroup analysis from the BIOSTEMI randomized trial, ultrathin‐strut BP‐SES were superior to thin‐strut DP‐EES with respect to TLF at 2 years among STEMI patients undergoing both complex and noncomplex pPCI.
Fulminant lupus pneumonitis complicating systemic lupus erythematosus in the elderly
Authors: Helali Wiem , El Euch Mounira , Sassi Cyrine , Kefi Asma , Ben Hamida Fethi , Ben Abdelghani Khaoula , Turki Sami , Abderrahim Ezzedine 
Published: February 2023

AbstractFulminant lupus pneumonitis is a rare complication of SLE. We report a case of 75 years‐old male patient with SLE who developed pneumonia and severe respiratory failure requiring mechanical ventilation. Refractory respiratory distress complicating noninfectious fulminant lupus pneumonitis did not respond to methylprednisolone and intravenous immunoglobulin treatment.
Structurally Ordered PtNi Intermetallic Nanoparticles as Efficient and Stable Cathode Catalysts for Proton Exchange Membrane Fuel Cells
Authors: Li Lubing , Zhang Lei , Zhai Tianyu , Yang Sheng , Wang Weiding , Zhou Di , Su Jinzhan , Guo Liejin 
Published: February 2023

AbstractExploring oxygen reduction reaction (ORR) catalysts with superior electrochemical performance and long‐term stability is crucial to the development of proton exchange membrane fuel cells (PEMFCs). In this work, graphited carbon with high specific surface area was obtained under relatively low temperature using Ni catalyst, then ordered nanoparticles (NPs) PtNi catalysts attaching to graphited carbon were synthesized via polyol reduction and thermal treatment. Benefiting from graphitized carbon support and appropriate order degree, PtNi/GC‐700 NPs catalyst exhibits excellent electrocatalytic ORR performance with specific and mass activities as high as 2.8‐fold and 3.7‐fold of the commercial Pt/C catalyst, respectively. Besides, the as‐prepared PtNi/GC‐700 catalyst exhibits superior stability with negligible degradation after 10000 potential cycles, due to its ordered chemical structure. The work described herein highlights the potential of structurally ordered electrocatalysts for efficient and durable fuel cell cathodic catalysts.
Detecting RNA–Protein Interactions With EGFP‐Cy3 FRET by Acceptor Photobleaching
Authors: Alam Shagufta Rehman , Mahadevan Mani S. , Periasamy Ammasi 
Published: February 2023

AbstractFörster Resonance Energy Transfer (FRET) is a great tool for cell biologists to investigate molecular interactions in live specimens. FRET is a distance‐dependent phenomenon which can detect molecular interactions at distances between 1‐10 nm. Several FRET approaches are reported in the literature for live and fixed cells to study protein‐protein interactions; this protocol provides details of acceptor photobleaching as a FRET method to study RNA‐Protein interactions. Cy3‐labeled RNA foci (FRET acceptors) are photobleached at the intra‐cellular site of interest (the nuclei) and the intensity of the EGFP‐tagged proteins (FRET donors) at that same site are measured pre‐ and post‐ photobleaching. In principle, FRET is detected if the intensity of EGFP increases after photobleaching of Cy3. This protocol describes necessary steps and appropriate controls to conduct FRET measurements by the acceptor photobleaching method. Successful applications of this protocol will provide data to support the conclusion that EGFP‐labeled proteins directly interact with Cy3‐labeled RNA at the site of photobleaching. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: FRET in fixed cellsAlternate Protocol: FRET in live cells
Substituent Effect on Ligand‐Centered Electrocatalytic Hydrogen Evolution of Phosphorus Corroles
Authors: Yang Gang , Ullah Zakir , Yang Wu , Wook Kwon Hyung , Liang Zhen‐Xing , Zhan Xuan , Yuan Gao‐Qing , Liu Hai‐Yang 
Published: February 2023

AbstractThere have been few reports on the substituent effect of main‐group‐element corrole complexes as ligand‐centered homogeneous electrocatalysts for the hydrogen evolution reaction (HER). The key to comprehend the catalytic mechanism and develop efficient catalysts is the elucidation of the effects of electronic structure on the performance of energy‐related small molecules. In this work, the “push‐pull” electronic effect of the substituents on electrocatalytic HER of phosphorus corroles was investigated by using 5,10,15‐tris(phenyl) corrole phosphorus (1P), 10‐pentafluorophenyl‐5,15‐bis(phenyl) corrole phosphorus (2P), 10‐phenyl‐5,15‐bis(pentafluorophenyl) corrole phosphorus (3P), 5,10,15‐tris(pentafluorophenyl) corrole phosphorus (4P) complexes bearing hydroxyl axial ligands and different numbers of fluorine atoms on themeso‐aryl substituents. The results revealed that the catalytic HER activity of phosphorus corroles decreased with the increasing of fluorine atom numbers, it follows in the order 1P>2P>3P>4P. Density functional theory (DFT) calculations show that the corrole 1P has the lowest free energy barrier in catalytic HER.
PX‐12 synergistically enhances the therapeutic efficacy of vorinostat under hypoxic tumor microenvironment in oral squamous cell carcinoma in vitro
Authors: Akhlaq Rafia , Khan Tajwali , Ahmed Tehmina , Musharraf Syed G. , Ali Arslan 
Published: February 2023

AbstractHypoxia is a characteristic feature of solid tumors, including oral squamous cell carcinoma (OSCC), which causes therapeutic resistance. The hypoxia‐inducible factor 1‐alpha (HIF‐1α) is a key regulator of hypoxic tumor microenvironment (TME) and a promising therapeutic target against solid tumors. Among other HIF‐1α inhibitors, vorinostat (suberoylanilide hydroxamic acid, SAHA) is a histone deacetylase inhibitor (HDACi) targeting the stability of HIF‐1α, and PX‐12 (1‐methylpropyl 2‐imidazolyl disulfide) is a thioredoxin‐1 (Trx‐1) inhibitor preventing accumulation of HIF‐1α. HDACis are effective against cancers; however, they are accompanied by several side effects along with an emerging resistance against it. This can be overcome by using HDACi in a combination regimen with Trx‐1 inhibitor, as their inhibitory mechanisms are interconnected. HDACis inhibit Trx‐1, leading to an increase in the production of reactive oxygen species (ROS) and inducing apoptosis in cancer cells; thus, the efficacy of HDACi can be elevated by using a Trx‐1 inhibitor. In this study, we have tested the EC50 (half maximal effective concentration) doses of vorinostat and PX‐12 on CAL‐27 (an OSCC cell line) under both normoxic and hypoxic conditions. The combined EC50 dose of vorinostat and PX‐12 is significantly reduced under hypoxia, and the interaction of PX‐12 with vorinostat was evaluated by combination index (CI). An additive interaction between vorinostat and PX‐12 was observed in normoxia, while a synergistic interaction was observed under hypoxia. This study provides the first evidence for vorinostat and PX‐12 synergism under hypoxic TME, at the same time highlighting the therapeutically effective combination of vorinostat and PX‐12 against OSCC in vitro.
Identification of bleeding points after bile duct biopsy using red dichromatic imaging during peroral cholangioscopy: A case report
Authors: Kimura Yusuke , Okano Naoki , Hoshi Kensuke , Iwata Shuntaro , Ujita Wataru , Yamada Yuto , Iwasaki Susumu , Takuma Kensuke , Ito Ken , Igarashi Yoshinori , Matsuda Takahisa 
Published: February 2023

AbstractRed dichromatic imaging is a new image‐enhancement technology that clarifies the differences in blood concentrations and improves the visibility of the bleeding point. A 71‐year‐old man was presented with a common bile duct stone, which was completely removed using electrohydraulic shock wave lithotripsy with peroral cholangioscopy. During peroral cholangioscopy, a nodular lesion was found at the confluence of the cystic duct, and a forceps biopsy was performed. It was difficult to confirm the bleeding point using white‐light imaging because of the pooling of blood. After switching to red dichromatic imaging mode 2 and washing the bile duct with saline solution, the bleeding point was observed in darker yellow than the surrounding blood, allowing the identification of the bleeding point. Red dichromatic imaging can be used in the future to maintain hemostasis during peroral cholangioscopy.
Evidence of greater severity of diabetic foot ulcers during COVID‐19 pandemic: A real‐life single‐centre cohort study
Authors: Radellini Stefano , Vigneri Enrica , Smeraldi Lucia , Dinoto Ettore , Guercio Giovanni , Richiusa Pierina , Almasio Piero Luigi , Guarnotta Valentina , Salzillo Riccardo , Giordano Carla 
Published: February 2023

AbstractAimsIn the Sars‐Cov‐2 pandemic era, patients with diabetes mellitus (DM) manifested more severe forms of Sars‐Cov‐2 with greater mortality than non‐diabetic patients. Several studies documented more aggressive forms of diabetic foot ulcers (DFU) during the pandemic period even though the results were not unanimously confirmed. The aim of this study was to evaluate the clinical‐demographic differences between a cohort of Sicilian diabetic patients hospitalised for DFU in the pre‐pandemic 3 years and a cohort of patients hospitalised in the pandemic 2 years.Materials and MethodsOne hundred and eleven patients from the pre‐pandemic period 2017–2019 (Group A) and 86 patients from the pandemic period 2020–2021 (Group B) with DFU, admitted to the division of Endocrinology and Metabolism of the University Hospital of Palermo, were retrospectively evaluated. The clinical assessment of the type, staging and grading of the lesion, and the infective complication from DFU was performed.ResultsNo differences in HbA1c values were observed between the two groups. Group B showed a significantly higher prevalence of male subjects (p = 0.010), neuro‐ischaemic ulcers (p < 0.001), deep ulcers with involvement of bones (p < 0.001), white blood count levels (p < 0.001), and reactive C protein (p = 0.001) compared to group A.ConclusionsOur data show that in the COVID‐19 pandemic, a greater severity of ulcers requiring a significantly greater number of revascularisations and more expensive therapy, but without an increase in the amputation rate, was observed. These data provide novel information on the impact of the pandemic on diabetic foot ulcer risk and progression.
Optimum estimation of series capacitors for enhancing distribution system performance via an improved hybrid optimization algorithm
Authors: Mohamed Mohamed Abd‐El‐Hakeem , Abdelaziz Almoataz Y. , Darwish Mohamed M. F. , Lehtonen Matti , Mahmoud Karar 
Published: February 2023

AbstractAs the load on distribution networks grows, system operators and planners are constantly challenged with the issue of voltage regulation or enhancing the quality of supply to customers at the load end of lengthy distribution lines. This paper presents the optimum determination of series capacitor units in a distribution system to maximize energy‐saving and enhance voltage levels. Interestingly, series capacitors can enhance the capability of transmission lines, reduce line losses, enhance the performance of buses with large induction motor loads and reduce voltage flicker. At the same time, the limitations of series compensation are taken into consideration while calculating its optimum values. To achieve the planning objective and optimal load flow objective, two strategies: The Improved Grey Wolf Optimization method (I‐GWO) and Tabu Search (TS), are hybridized to get the benefit of their advantages. The I‐GWO has a movement strategy called dimension learning‐based hunting for enhancing the balance between global and local search and maintaining diversity. The proposed (I‐GWO‐TS) algorithm can solve mixed‐integer programming to achieve the planning and the optimal load flow objectives. The proposed method can be applied to a real Egyptian distribution system that is heavily loaded, with poor voltage regulation, and also has high‐power losses. The obtained results demonstrate the capability of the proposed approach to determine optimal series capacitors’ location and sizing for maximization of energy saving. Further, the proposed method improves the network performance regarding the voltage profile and power losses, although the limitations of including series compensation were considered in the distribution system.
Does self‐face awareness influence green building project performance?—An empirical evidence from China
Authors: Xie Xiaojun , Chen Xinyi , Wang Chaofan , Shuai Chuanmin , Zhu Min , Chen Yongxia 
Published: February 2023

AbstractIn the circumstances of “Carbon Peak,” it is imminent to implement green construction technology in the construction industry as it is a major source of CO2 emissions. Nevertheless, little study has been done on the impact of self‐face awareness (SA) on green building project performance (GP). On the basis of a large‐scale questionnaire survey in China and an in‐depth analysis of the four categories (cognition, behavior, satisfaction, and performance), this paper has added an SA dimension, and proposed a new impacting path. Then, based on the improved conceptual model for measuring the impact of green construction behavior on GP, this paper has conducted empirical tests by using a large sample of questionnaire data and partial least square‐structural equation modeling method. The results show that: (1) green construction behavior has a positive impact on GP, and the impact of perceived behavioral control of green construction technology is most significant for promoting green construction in the industry, and SA has a significant positive effect on green construction in the industry; (2) environmental perception and perceived behavioral control for green construction technology play a mediating role; and (3) significant differences indeed exist in the multigroup analysis, and the biggest difference is working experiences in the engineering field. Finally, this paper has proposed corresponding management implications based on the above findings. The main contribution of this study is that this study has developed a new dimension (SA) to the model considering Chinese national and cultural differences, in an attempt to reveal the impact of green construction technology on GP in the Chinese context. In addition, the promotion of green construction (PGC) in the construction industry was also added based on the sustainability perspective, which is the first attempt to explore the role of the PGC.
Fast multicriteria network selection scheme using hybrid of cat swarm optimization and TOPSIS algorithm for optimal handover in VANET
Authors: Jahangeer Naskath 
Published: February 2023

AbstractFuture generation vehicular networks will grant the technique for efficient data dissemination over dense heterogeneous radio technologies by considering existing communication technology to attain higher spectral competence for successful data rates. Due to higher mobility schemes, a vehicle frequently switches among different heterogeneous networks, which leads to unnecessary handovers. In this scenario, an amateurish handover algorithm delivers data with heavy packet loss, caused by frequent and ping‐pong handover. Hence, this article presents the model for. A network simulator is used to prove the performance of the algorithm. The performance of the algorithm is analyzed in terms of vehicle density, velocity, throughput, packet loss, and jitter.