bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endocytosis-mediated healing: recombinant human collagen type III chain-induced wound healing for scar-free recovery
Authors: Jin Jian , Li Hai-Hang , Chen Zheng-Li , Liu Qing-Song , Chen Ji-Qiu , Tao Zi-Han , Hong Xu-Dong , Ding Yin-Jia , Zhou Yue , Chen Ai-Fen , Zhang Xu-Dong , Lv Kai-Yang , Zhu Liang-Liang , Zhu Shi-Hui 
Published: January 2025

Abstract
Scar formation can be effectively prevented when the proportion of collagen type I (Col I)/type III (Col II) is reduced. Unlike Col III, recombinant human collagen type III chain (RHC III chain) does not possess a triple helical structure. This study aimed to elucidate the capacity of fibroblasts to uptake RHC III chain, reduce the Col I/Col III ratio, and determine its effects on wound healing and scar. RHC III chain demonstrates qualified cell compatibility. In cell experiments, immunofluorescence and Western Blot (WB) analyses revealed an increase in the polyhistidine tag level, indicating that RHC III chain in internalized by these cells. Transmission electron microscopy (TEM) showed increased intracellular phagocytic activity, indicating that RHC III chain enters fibroblasts by endocytosis. The immunofluorescence and WB showed that Col III synthesis enhanced, and Col I/Col III ratio reduced. However, the polyhistidine tag disappeared with time, indicating that RHC III chain degraded within cells and then synthesized into Col III. The content of newly synthesized Col III increases, but Real-time fluorescence quantitative showed a decrease in Col III related gene content suggests the formation of negative feedback. However, due to the sufficient raw materials, the amount of Col III synthesis is still increasing, leading to the reduction of the ratio of type I collagen/type III collagen, which beneficial to wound healing and reduce scar hyperplasia. In animal experiments, the SD rat full-thickness skin defect model of Wound suggests that RHC III chain also takes effect through endocytosis and ultimately promotes wound healing. The rabbit ear scar model suggests that RHC III chain inhibits scar proliferation by reducing the ratio of Col I/Col III. In summary, RHC III chain was endocytosed by fibroblasts to promote native Col III synthesis, as well as promote wound healing and reduce scar hyperplasia.
Contemporary uses of “lethal” or “life limiting” terminology in perinatal research
Authors: Gatta Luke A. , McCarthy Allison M. , Osmundson Sarah S. 
Published: January 2025

Purpose of review
A life-limiting fetal diagnosis (LLD) refers to a medical condition identified during pregnancy that is expected to lead to stillbirth, preclude ex utero survival, or significantly reduce neonatal life expectancy. The terms ‘lethal’ or ‘life-limiting’ are used to prognosticate early death for various anatomic or physiologic causes, although the expected timeframe is nonspecific. The purpose of this manuscript is to review how the terms ‘lethal’ or ‘life limiting’ are used in contemporary perinatal research.

Recent findings
Depending on the study design, ‘life-limiting’ is defined either prior to data analysis (such as prospective cohort studies), or after outcomes are assessed (such as case series). When ‘life-limiting’ is defined prior to data analysis, study-specific specific definitions may include timeframes from birth to death, probability of neonatal mortality, or a list of diagnoses based off billing codes.

Summary
Professional societies have guidelines to standardize the reporting of vital statistics, including early death. While these fall short of defining LLDs comprehensively, they present an opportunity for more specific prognostication following prenatal diagnosis, which may improve research standardization to facilitate a clearer understanding of LLDs in clinical practice.
Comparing the cost of cirrhosis to other common chronic diseases: A longitudinal study in a large national insurance database
Authors: Obradović Filip , Vitello Dominic J. , Hasjim Bima J. , Obayemi Joy , Polineni Praneet , Gmeiner Michael , Koep Eleena , Jain Aditya , Crippa Federico , Duarte-Rojo Andrés , Rohan Vinayak S. , Kulik Laura , Doll Julianna M. , Banea Therese , McNatt Gwen E. , Zhao Lihui , VanWagner Lisa B. , Manski Charles F. , Ladner Daniela P. 
Published: January 2025

Background and Aims:
Cirrhosis prevalence is increasing, yet costs associated with its chronic, complex care are poorly understood. The aim was to characterize the costs of care for patients with cirrhosis and compare them to other chronic diseases such as heart failure (HF) and chronic obstructive pulmonary disease (COPD), for which the public health burden is better recognized.

Approach and Results:
Patients enrolled in Medicare Advantage plans from a large national insurer between 2011 and 2020 with cirrhosis, HF, and COPD were identified by ICD-9/-10 codes. Costs (USD) of care were calculated per patient-month and included inpatient medical, emergency medical, pharmacy, and other costs. In all, 93,308 patients with cirrhosis, 355,520 patients with HF, and 318,949 patients with COPD were analyzed. Patients with cirrhosis, HF, and COPD had a mean (SD) age of 69.6 (9.5), 75.9 (9.7), and 72.9 (9.8) years, respectively. The most frequent etiologies were metabolic dysfunction–associated steatohepatitis (37.7%) and alcohol-associated cirrhosis (22.1%). The total monthly cost of care for patients with cirrhosis, HF, and COPD was $3032.00, $2491.60, and $1955.60 respectively. The cost for patients with cirrhosis exceeded that for HF by $540.40 (21.7% higher) and COPD by $1076.30 (55.0% higher). The monthly cost of care for decompensated cirrhosis was $3969.30, which was 59.3% ($1477.70) higher than for HF and 103.0% ($1,955.60) higher than for COPD.

Conclusions:
The cost of care for cirrhosis is high, significantly higher than HF and COPD. Interventions directed at optimizing care to prevent progression to cirrhosis and decompensation are likely to alleviate this public health burden.
Associations among tooth loss, denture use, and dementia in older Taiwanese adults
Authors: Chen Yi-Wen , Li Chung-Yi , Lee Shang-Chi , Ku Li-Jung Elizabeth , Chou Yu-Tsung , Hargono Arief , Chiu Chang‑Ta , Hou Wen-Hsuan 
Published: January 2025

Background:
The literature has mainly focused on the association between tooth loss and dementia, and evidence regarding the separate and joint effects of tooth loss and denture use on the risk of dementia is limited. This study investigated the links among tooth loss, denture use, and dementia risk in Taiwanese adults aged 65 years or older.

Methods:
This retrospective cohort study utilized data from the 2009 National Health Interview Survey and 2008–2017 National Health Insurance claim records, including the records of 2247 individuals free from dementia treatment in 2008–2009. Dementia diagnoses were tracked until 2017. The Cox proportional hazards model was used to calculate hazard ratios (HRs) for exploring the correlations among tooth loss, denture use, and dementia risk.

Results:
Although older adults who had 20 or more natural teeth and used dentures had the lowest dementia incidence (1.43 per 100 person-years) among participants, those with fewer than 20 natural teeth, with or without dentures, experienced high dementia incidence rates of 2.31 and 3.33 per 100 person-years, respectively. After adjusting for sociodemographic characteristics, health conditions/comorbidities, and lifestyles, having fewer than 20 natural teeth (vs. ≥20 remaining natural teeth) or not using dentures (vs. ≥20 remaining natural teeth) was found to be associated with increased but not statistically significant HRs of 1.299 (95% confidence interval [CI], 0.986–1.710) and 1.199 (95% CI, 0.945–1.521). Subgroup analysis revealed that compared with older adults with 20 or more natural teeth and dentures, those with fewer than 20 natural teeth and not wearing dentures had a significantly increased risk of dementia (adjusted HR, 1.569; 95% CI, 1.038–2.370).

Conclusion:
This study found that having fewer than 20 natural teeth and not using dentures was significantly associated with an increased risk of dementia in Taiwanese individuals aged 65 years or above.
Combined single-photon emission computed tomography-myocardial perfusion imaging with coronary calcium score for assessing coronary disease
Authors: Calandri Enrico , Verdoia Monica , Sirovich Roberta , Giraudo Maria Teresa , Pultrone Mirco , Frantellizzi Viviana , Viola Orazio , Crivelli Francesca , Gallina Sonya , Serralunga Monica , Rognoni Andrea , De Vincentis Giuseppe 
Published: January 2025

Purpose
Coronary artery disease (CAD) underestimation represents a major pitfall of single-photon emission computed tomography-myocardial perfusion imaging (SPECT-MPI). Coronary artery calcium score (CACS) has emerged as a sensitive tool for the assessment of suspect CAD; however, the integration of SPECT-MPI with CACS has been seldom evaluated, so far, and was therefore the aim of the present study.

Methods
Patients undergoing SPECT-MPI with CACS and subsequent coronary angiography were included. ROC curves were used to identify the CACS values best predictive for CAD. In SPECT-MPI negative patients, the formula:

defined the optimal CACS cut-points. The Systematic Coronary Risk Evaluation 2 was applied for 10-year cardiovascular risk estimation. Significant CAD was defined for an epicardial coronary stenosis >70 or 50% for the left main.

Results
Among 124 patients, 61 (49.19%) displayed positive SPECT-MPI, whereas 69 (56%) had significant CAD at angiography. Sensitivity, specificity, and positive predictive value (PPV) for SPECT-MPI were, respectively, 74, 82, and 84%. Considering 63 SPECT-MPI negative cases, the index values for CACS at the optimal cutoff value of 1949 were: sensitivity 28%, specificity 89%, and PPV 50%, allowing to further detect five (8%) of the patients with significant CAD. The increased discriminative power of the combined SPECT-MPI with CACS was not conditioned by the pretest cardiovascular risk.

Conclusion
Among patients with suspect CAD undergoing SPECT-MPI, the addition of CACS in negative cases allows to detect a consistent further 8% of patients with significant CAD, thus limiting the risk of disease underestimation and offering potential prognostic benefits.
Molecular and Microscopy Evaluation of Lymphatic filariasis in Ilorin Metropolis, Kwara State Nigeria
Authors: Omah Prosper , Nmorsi Onyebiguwa Patrick Goddey , Egwunyenga Andy Ogochukwu 
Published: January 2025

ABSTRACTBackgroundLymphatic filariasis is widespread and a major public health problem in many developing countries with warm and humid climate and it is one of the most prevalent neglected tropical diseases. This study, evaluated the diagnostics performance of molecular and conventional microscopy method of Lymphatic filariasis in Ilorin Metropolis, Kwara State Nigeria.MethodsGenomic DNA was collected from 45 human blood samples obtained from Ilorin, Kwara State. They were then amplified by PCR and detected through gel electrophoresis. The PCR products were purified with QIAquick PCR purification kit (Qiagen, Valencia, CA) and then DNA quantified for Sanger sequencing, Bio Edit Software were used for sequence alignment and editing. 37 sequences were selected for analysis by MEGA X software to access the evolutionary connection of W. bancrofti isolates.Results37 (82.2%) PCR products were positive for W. bancrofti and 16 (35.6%) were Positive for Microscopy. Hence, the sensitivity of PCR (82.2%) while the specificity was (64.4%).With the microscopy, the chances of a negative samples turning out positive by PCR, also known as the positive predictive value (PPV) was (69.8%) while the chances of being picked as truly negative predictive value was (78.4%). In assessing the level of agreement show that microscopy has a weak agreement, Kappa index statistics (0.46) with molecular techniques 95% CI,0.2-0.4).ConclusionSince Polymerase Chain Reaction showed higher sensitivity (82.2%) as positive predictive value, it is therefore recommended to include PCR as complementary routine diagnosis for lymphatic filariasis especially subclinical cases due to W. bancrofti despite its relatively higher cost.
Five-Year Survival Outcomes for Breast Cancer Patients Across Continental Africa: A Contemporary Review of Literature with Meta Analysis
Authors: Badu-Peprah Augustina , Kontor Ernest Kissi , Benjamin Adu-Gyamfi , Kumah Jessica , Essuman Akosua Aya , Selorm Bossoh , Nurudeen Issahak , Owusu Bismark Osei , Jain Nityanand 
Published: January 2025

AbstractBackgroundBreast cancer associated mortality in Africa remains high due to poor survival rates, varying widely across countries. Despite medical advancements, barriers like limited access to early detection and treatment persist. This meta-analysis offers a crucial update on 5-year survival trends and influencing factors across continental Africa.MethodsA systematic search of four biomedical databases and citation searching identified 79 articles from 22 African countries, analyzing 27,559 patients (97% female). A random-effects model was used to estimate the 5-year survival rate with subgroup analyses. Publication bias was assessed using Egger’s test and funnel plots.ResultsPooled overall 5-year breast cancer survival in Africa averaged 48% (95% CI: 43-53%) with high statistical heterogeneity (I² = 98%). Survival was highest in Northern Africa (64%; 95% CI: 59-69%) and lowest in Western Africa (32%; 95% CI: 23-42%). Males (51%; 95% CI: 36-65%) had marginally higher survival than females (48%; 95% CI: 42-54%). Socioeconomic indices were positively associated with better outcomes. Publication bias, adjusted by the trim-and-fill method, raised survival to 62% (95% CI: 55-67%). A country-wise comparison with 2018 estimates suggests a declining survival tendency, with WHO AFRO countries reporting the poorest estimates among other WHO regions. Despite regional differences, survival trends seem to have plateaued near 48-49% level continent-wide since the early 2010s.ConclusionsOur findings reveal marked regional disparities in survival rates across Africa, underscoring the urgent need for targeted healthcare interventions. Strengthening healthcare systems, ensuring universal access, and driving socioeconomic progress are vital to improving survival outcomes.
Determination of the mechanisms of MCPA resistance inAmaranthus powellii
Authors: Aicklen Isabelle , Jugulam Mithila , Gaines Todd , Kramer William , Laforest Martin , Robinson Darren , Sikkema Peter , Tardif François 
Published: January 2025

AbstractResistance to 2-methyl-4-chloro-phenoxyacetic acid (MCPA) was recently confirmed in a population of green pigweed (Amaranthus powellii) from Dresden, Ontario, Canada, with a resistance factor of 4.4. Resistance to synthetic auxin herbicides inAmaranthusspecies has previously been linked to non-target site resistance mechanisms with low-level resistance factors (<10). Based on this information an investigation into the mechanism of resistance to MCPA was conducted in this population of green pigweed. No significant differences in absorption, translocation, and metabolism of14C-MCPA existed between the resistant and a susceptible population of green pigweed. An RNA-Sequencing experiment to identify differentially expressed genes also confirmed this result. Genes that were differentially expressed in the resistant population were linked to target site modifications. A single nucleotide polymorphism (SNP) conferring a leucine to phenylalanine substitution was identified in auxin response factor (ARF) 9. This mutation may be in the Phox and Bem1p (PB1) domain in ARF9 which facilitates the interaction between ARFs and Aux/IAA repressor proteins. The results demonstrate that the mechanism of resistance to MCPA is not a non-target site mechanism and may be linked to a target site modification. Specifically, a SNP in ARF9 could disrupt the interaction between ARF9 and other Aux/IAAs which could prevent ubiquitination of Aux/IAAs and subsequent lethal action of MCPA.
Strategic Conservation Prioritization Through Creating and Updating the Regional Species of Greatest Conservation Need (RSGCN) List in the Northeastern U.S.
Authors: Starking Melissa D. , Rice Tracy Monegan , Terwilliger Karen , Drummey Donovan , Frantz Mack , Haffner Catherine D. , Hess Brian , Holst Lisa , Marchand Michael , Burgio Kevin R. 
Published: January 2025

AbstractThrough the end of the century, biodiversity is expected to markedly decline around the world due to climate change, habitat loss, and other factors. Because of this, there is an increasing need for more efficient, effective, and collaborative conservation efforts worldwide. While many global and national threat assessment databases exist, they may only have limited utility at regional and local scales where on-the-ground conservation is best implemented. To better identify species risk at a more regional scale, the 14 state fish and wildlife agencies in the northeastern United States worked with taxonomic experts across the Northeast to create the most recent (2023) list of Regional Species of Greatest Conservation Need (RSGCN). We compared a preliminary list compiled from global and national datasets to the finalized list after the process documented here. We found that the global and national datasets only identified ∼55% of the 806 species statuses accurately for the region, demonstrating the importance of current local and regional expertise and data. The process of identifying RSGCN for the northeastern U.S. has resulted in specific actionable information leading to beneficial outcomes, such as dedicated funding for regional initiatives, data-sharing, and coordination among regional, state, and local conservation organizations - enabling beneficial conservation outcomes across the region for many of the species and habitats of the region. The methods described may serve as a framework for other regions to help identify conservation targets using the best available and more localized landscape- and watershed-scale information.
Developing a model system to sustain ex vivo chloroplast function
Authors: Mohagheghi Mariam , Navid Ali , Mossington Thomas , Ye Congwang , Coleman Matthew , Honag-Phou Steven 
Published: January 2025

AbstractChloroplasts are critical organelles in plants and algae responsible for accumulating biomass through photosynthetic carbon fixation and cellular maintenance through metabolism in the cell. Chloroplasts are increasingly appreciated for their role in biomanufacturing, as they can produce many useful molecules, and a deeper understanding of chloroplast regulation and function would provide more insight for the biotechnological applications of these organelles. However, traditional genetic approaches to manipulate chloroplasts are slow, and generation of transgenic organisms to study their function can take weeks to months, significantly delaying the pace of research. To develop chloroplasts themselves as a quicker and more defined platform, we isolated chloroplasts from the green algae,Chlamydomonas reinhardtii, and examined their photosynthetic function after extraction. Combined with a metabolic modeling approach using flux-balance analysis, we identified key metabolic reactions essential to chloroplast function and leveraged this information into reagents that can be used in a “chloroplast media” capable of maintaining chloroplast photosynthetic function over timeex vivocompared to buffer alone. We envision this could serve as a model platform to enable more rapid design-build-test-learn cycles to study and improve chloroplast function and potentially as a foundation for the bottom-up design of a synthetic organelle-containing cell.
Highly divergent mesnidoviruses found in TSA database, especially in spiders
Authors: Xie Jiazheng , Zhang Yu 
Published: January 2025

SUMMARYThe mesnidoviruses are positive single-stranded viruses belonging to the orderNidovirales. They were mostly found in mosquitoes, and represent a link from small to large nidoviruses. In this study, we report 18 newly discovered nidoviruses in TSA database, most of them are from spiders (OrderArachnida). After reassembly, we got 9 nearly complete genomes with sizes ranged from ∼19,500 to 33,500 nt. Further phylogenetic analyses assigned these nidoviruses toMesnidovirineae. Among them, the spider viruses represent a new clade. Pairwise evolutionary distance (PED) analysis showed these mesnidoviruses are highly divergent, with PED values from 2.2 to 4.9 with existing ones. Even among themselves the PED values are notable: with most exceeding the threshold of 0.32 that defines a new virus genus, suggesting these viruses are new species or belong to new higher-ranking taxa. In summary, we reported 18 mesnidoviruses in spiders, insects, plant andAnthozoa, the highly divergence suggested that spiders may harbor diverse mesnidoviruses. As the first nidovirus reported inArachnidaandAnthozoa, this discovery will contribute to the evolution study ofNidovirales.
Next-Generation Mapping of the ACINUS-Mediated Alternative Splicing Machinery and Its Regulation by O-glycosylation inArabidopsis
Authors: Shrestha Ruben , Reyes Andres V , Carey Shane , Karunadasa Sumudu S , Zhai Wenxuan , Byun Danbi , Lin Wen-Dar , Li Jie , Alerte Kathrine , Cui Hongchang , Wang Zhi-Yong , Xu Shou-Ling 
Published: January 2025

AbstractAlternative splicing (AS) is a key mechanism of gene regulation, but the full repertoire of proteins involved and the regulatory mechanisms governing this process remain poorly understood. Using TurboID-based proximity labeling coupled with mass spectrometry (PL-MS), we comprehensively mapped the Arabidopsis AS machinery, focusing on the evolutionarily conserved splicing factor ACINUS, its paralog PININ, and the stable interactor SR45. We identified 298 high-confidence components, including both established and novel interactors, providing strong evidence that alternative splicing is coupled to transcription and that multiple RNA processing steps occur simultaneously in plants. Selected interactors were validated through AS readouts and phenotypic analysis. Furthermore, a systematic evaluation of O-glycosylation double mutants revealed that SECRET AGENT (O-GlcNAc transferase) and SPINDLY (O-fucose transferase) modulate AS through both ACINUS-dependent and -independent pathways. Our results unravel the plant AS network and highlight the global role of sugar modification in RNA processing.