bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of foot care knowledge and behaviour of older people with type 2 diabetes in Beijing community and analysis of influencing factors
Authors: Li Gaoqiang , Lu Qian , Wen Bing , Qi Xin , Guan Hui , Li Huijuan , Liu Jin , Ding Yanming 
Published: January 2025

AbstractGood foot care knowledge and behaviour are very important to prevent the occurrence of diabetic foot, but there are few reports on the foot care knowledge and behaviour of older people with diabetes in the community. The purpose of this study was to understand the foot care knowledge and behaviour of older people with type 2 diabetes in Beijing community, and analyse its influencing factors, so as to provide reference for further intervention. We investigated 254 older people with type 2 diabetes in Xinjiekou community, Beijing, including their general information, chronic complications, foot care knowledge and behaviour. The results showed that the average scores of foot care knowledge and behaviour were 73.38 ± 12.25 and 49.70 ± 8.70, respectively. Multiple stepwise regression analysis showed that the factors affecting the total score of foot nursing knowledge of older people with diabetes in community were gender, duration of diabetes and whether they had received foot nursing education (p < 0.05). The factors influencing the total score of foot nursing behaviour were gender, duration of disease, whether they had received foot nursing education and peripheral vascular disease (p < 0.05). In conclusion, the knowledge of foot care of older people with diabetes in community is in the middle level, and the foot care behaviour is not optimistic. Community healthcare workers can improve patients' knowledge of foot care and improve their compliance with foot care behaviour through foot care health education. At the same time, we should pay more attention to men, those with a shorter duration of diabetes and diabetic patients with peripheral vascular disease to reduce the occurrence of diabetic foot.
Visions of the Future of Craft Education
Authors: Mommo Sanna , Kouhia Anna , Rönkkö Marja‐Leena 
Published: January 2025

AbstractThis study examines the history of craft education, especially in the context of social and temporal changes, and envisions its future based on narratives collected by student teachers at two Finnish universities. The research material covers essay responses that discussed the future of craft education in the light of forecasted megatrends. The essay responses were analysed using narrative methods, and the results are presented as visions of the priorities and content of future craft education. While craft teaching today emphasises technology, well‐being and communal living, the growth of digitality, sustainable education and materiality is likely to continue in the future. We envision that future craft education will focus on pedagogical content rooted in craft traditions, with a strong emphasis on skills education.
Loss of FTH1 Induces Ferritinophagy‐Mediated Ferroptosis in Anaemia of Myelodysplastic Syndromes
Authors: Yang Liyan , Zhang Mengying , Liu Mengyuan , Yu Yating , Zhang Yue , Yang Jinyue , Xing Limin , Shao Zonghong , Wang Huaquan 
Published: January 2025

ABSTRACTSingle‐cell sequencing of lineage negative (Lin‐) cells from patients with myelodysplastic syndromes (MDS) revealed a reduction in ferritin heavy chain 1 (FTH1) levels, yet the significance of this decrease in FTH1 in the pathophysiology of MDS remains unclear. In this study, we evaluated the role of FTH1 in patients with MDS. The mRNA expression of FTH1 in GlycoA+ nucleated erythrocytes from MDS patients was significantly lower than that in control group. FTH1 was implicated in both ferritinophagy and ferroptosis in MDS patients, processes that are linked to the development of anaemia. To further validate our observations, we employed shRNA to knock down the FTH1 gene in K562 and SKM1 cells. This knockdown confirmed that the elevated ferroptosis levels observed after FTH1 depletion were indeed due to the induction of ferritinophagy. Hemin stimulation promoted the differentiation of K562 cells, while downregulation of FTH1 gene expression had an impact on erythroid differentiation and haemoglobin synthesis. Taken together, our results suggest that FTH1‐mediated ferritinophagy may represent a novel therapeutic target for MDS.
Enhancing Molecular Network‐Based Cancer Driver Gene Prediction Using Machine Learning Approaches: Current Challenges and Opportunities
Authors: Zhang Hao , Lin Chaohuan , Chen Ying'ao , Shen Xianrui , Wang Ruizhe , Chen Yiqi , Lyu Jie 
Published: January 2025

ABSTRACTCancer is a complex disease driven by mutations in the genes that play critical roles in cellular processes. The identification of cancer driver genes is crucial for understanding tumorigenesis, developing targeted therapies and identifying rational drug targets. Experimental identification and validation of cancer driver genes are time‐consuming and costly. Studies have demonstrated that interactions among genes are associated with similar phenotypes. Therefore, identifying cancer driver genes using molecular network‐based approaches is necessary. Molecular network‐based random walk‐based approaches, which integrate mutation data with protein–protein interaction networks, have been widely employed in predicting cancer driver genes and demonstrated robust predictive potential. However, recent advancements in deep learning, particularly graph‐based models, have provided novel opportunities for enhancing the prediction of cancer driver genes. This review aimed to comprehensively explore how machine learning methodologies, particularly network propagation, graph neural networks, autoencoders, graph embeddings, and attention mechanisms, improve the scalability and interpretability of molecular network‐based cancer gene prediction.
Determinants of Problem‐Solving Skills of Nursing Students: Solution‐Focused Thinking Skills and Emotional Intelligence
Authors: Selçuk Tosun Alime , Akgül Gündoğdu Nurcan , Avci Dilek , Gündüz Emine Selda 
Published: January 2025

ABSTRACTBackgroundProblem‐solving skills are some of the leading strategies for dynamism in the content and quality of nursing care.AimThe present study is aimed at determining nursing students' problem‐solving, solution‐focused thinking, and emotional intelligence levels and investigating the relationship between their problem‐solving skills and these variables.MethodsThe study comprised 305 nursing students in Turkey. Data were collected between May and June 2021, using the personal information form, problem‐solving inventory, solution‐focused inventory and Schutte emotional intelligence scale. One‐way ANOVA, t‐test and multiple regression were used for data analysis.ResultsThe mean age of the participants was 21.81 ± 5.93 years, 75.7% of them were women, 35.1% were senior students and 58.0% perceived their academic achievement as moderate. The mean scores from the overall problem‐solving inventory, solution‐focused inventory, and emotional intelligence scale were 92.93 ± 19.30, 50.26 ± 8.43 and 128.22 ± 14.11, respectively. Variables such as sex, perceived academic achievement, emotional intelligence and solution‐focused thinking levels affected problem‐solving skills (p < 0.01). The effective predictive factors accounted for 50.2% of the change in participants' problem‐solving skill levels.ConclusionFor this reason, revising the national nursing curriculum with programmes that develop emotional intelligence and solution‐focused thinking skills will enable nursing students to better manage the nursing process in clinical and field practices.
Prenatal Valproic Acid Induces Autistic‐Like Behaviors in Rats via Dopaminergic Modulation in Nigrostriatal and Mesocorticolimbic Pathways
Authors: Cezar Luana C. , da Fonseca Caio Cesar N. , Klein Marianne O. , Kirsten Thiago B. , Felicio Luciano F. 
Published: January 2025

ABSTRACTAutism spectrum disorder (ASD) is a complex developmental disorder characterized by several behavioral impairments, especially in socialization, communication, and the occurrence of stereotyped behaviors. In rats, prenatal exposure to valproic acid (VPA) induces autistic‐like behaviors. Previous studies by our group have suggested that the autistic‐like phenotype is possibly related to dopaminergic system modulation because tyrosine hydroxylase (TH) expression was affected. The objective of the present study was to understand the dopaminergic role in autism. Wistar rats on gestational day 12.5 received VPA (400 mg/kg) and behaviors related to rat models of ASD were evaluated in juvenile offspring. Neurochemical and genetic dopaminergic components were studied in different brain areas of both juvenile and adult rats. Prenatal VPA‐induced autistic‐like behaviors in comparison to a control group: decreased maternal solicitations by ultrasonic vocalizations, cognitive inflexibility and stereotyped behavior in the T‐maze test, decreased social interaction and play behavior, as well as motor hyperactivity. Prenatal VPA also decreased dopamine synthesis and activity in the striatum and prefrontal cortex, as well as dopamine transporter, D1 and D2 receptors, and TH expressions. Moreover, prenatal VPA increased TH+ immunoreactive neurons of the ventral tegmental area–substantia nigra complex. In conclusion, the dopaminergic hypoactivity associated with the behavioral impairments exhibited by the rats that received prenatal VPA suggests the important role of this system in the establishment of the characteristic symptoms of ASD in juvenile and adult males. Dopamine was demonstrated to be an important biomarker and a potential pharmacological target for ASD.image
Poststroke Translocator Protein Expression Dynamics and Correlations to Chronic Infarction: A [123I]‐CLINDE‐SPECT Study
Authors: Jensen Per , Ozenne Brice , Meden Per , Feng Ling , Thomsen Gerda , Knudsen Lars , Steglich‐Arnholm Henrik , Møller Kirsten , Thomsen Carsten , Svarer Claus , Beliveau Vincent , Mikkelsen Jens , Knudsen Gitte , H Pinborg Lars 
Published: January 2025

ABSTRACTBackground and PurposeThis study aims to investigate the longitudinal changes in translocator protein (TSPO) following stroke in different brain regions and potential associations with chronic brain infarction.MethodsTwelve patients underwent SPECT using the TSPO tracer 6‐Chloro‐2‐(4ʹ‐123I‐Iodophenyl)‐3‐(N,N‐Diethyl)‐Imidazo[1,2‐a]Pyridine‐3‐Acetamide, as well as structural MRI, at 10, 41, and 128 days (median) after ischemic infarction in the middle cerebral artery. TSPO expression was measured in lesional (MRI lesion and SPECT lesion), connected (pons and ipsilesional thalamus), and nonconnected (ipsilesional cerebellum and contralesional occipital cortex) regions. Correlations were explored between the volume of chronic infarction and TSPO expression in nonconnected regions of interest (ROIs) at 128 daysResultsThroughout the study period, TSPO levels decreased by 24%–33% in lesional ROIs, while levels increased in connected ROIs by 35%–69% and in nonconnected ROIs by 53%–77%. At 128 days poststroke, TSPO expression in ipsilesional cerebellum positively correlated with chronic infarction volume (p = 0.002, r2 = 0.72).ConclusionsThis study expands the current knowledge of spatial and temporal TSPO expression in humans by quantifying TSPO changes in lesional, connected, and nonconnected brain regions at three time points after cerebral infarction as well as correlating late‐stage TSPO upregulation and chronic infarction volume.
Utilizing Quantitative Analysis of CSF Volume from Clinical T1‐Weighted MRI to Predict Thrombectomy Outcomes
Authors: Kawas Mohammad I. , Shamulzai Ahmad , Atcheson Kyle M. , Horn Alex C. , Ma Renate , Kittel Carol , Curry Brian , Lipford Megan , Kim Jeongchul , Solingapuram Sai Kiran K. , Wolfe Stacey Q. , Whitlow Christopher T. 
Published: January 2025

ABSTRACTBackground and PurposeEndovascular thrombectomy (EVT) is the standard for acute ischemic stroke from large vessel occlusion, but post‐EVT functional independence varies. Brain atrophy, linked to higher cerebrospinal fluid volume (CSFV), may affect outcomes. Baseline CSFV could predict EVT benefit by assessing brain health. We aimed to quantify total CSFV from clinical T1‐weighted (w) magnetic resonance imaging (MRI) to assess global brain atrophy and its association with functional outcomes following successful EVT.MethodsWe performed a retrospective analysis of patients achieving thrombolysis‐in‐cerebral‐infarction ≥2b revascularization via prospectively maintained single‐institution stroke thrombectomy registry (n = 432) between 2015 and 2021. We included 214 patients (mean age 67.5 ± 14.6, 49% female) with acceptable quality MRI within 14 days of EVT and available modified Rankin‐scale (mRS) at 90 days post EVT. Clinical T1w images were transformed into high‐resolution images using the convolutional neural‐network SynthSR. FreeSurfer software was then used to estimate total cranial CSFV. To correct for head size, percentage of CSFV to intracranial volume was used.ResultsBaseline CSFV% significantly predicted 90‐day mRS in an ordinal regression model adjusted for baseline mRS (p < 0.001). Further modeling was performed to account for age, sex, 24‐h National‐Institutes‐Health‐Stroke‐Scale (NIHSS), smoking history, prior stroke, hypertension, congestive heart failure, hemoglobin‐A1c, atrial fibrillation, and Alberta‐Stroke‐Program‐Early‐CT‐Score (ASPECTS). Total CSFV% remained an independent predictor of 90‐day mRS (p = 0.012). CSFV% did not significantly predict the occurrence of any type of hemorrhagic transformation in a logistic regression model.ConclusionsIncreased CSFV% correlates with poorer functional outcomes post EVT. Total CSFV% may serve as a useful imaging biomarker for clinicians determining patient prognostication prior to EVT.
Identification, cellular localization, and function analysis of a novel toll‐like receptor (PtToll4) in Portunus trituberculatus
Authors: Liu Hao , Shu Feng‐Ling , Zhang Dan‐Feng , Yin Fei , Zhou Su‐Ming 
Published: January 2025

AbstractToll‐like receptor (TLR) is one of the most important pattern recognition receptors in both invertebrate and vertebrate animals. In previous study, three different kinds of TLR cDNAs (PtToll1‐3) have been cloned from Portunus trituberculatus. In this study, a novel PtToll4 cDNA sequence was identified in this crab. The PtToll4 ORF is predicted to encode 894 peptides with an initiating signal peptide, an extracellular LRRs domain, a transmembrane region, and an intracellular toll‐interleukin‐1 receptor domain. Based on the sequence and phylogenetic analysis, PtToll4 distinctly clustered with almost all crustacean tolls, which mostly clustered with some shrimp TLRs and Eriocheir sinensis Toll 2 (EsToll 2). The real‐time quantitative polymerase chain reaction analysis showed that the transcript of PtToll4 was constitutively expressed in tissues. In hemocytes, the transcript of PtToll4 in large granulocytes is approximately threefold higher than in small granulocytes and hyalocytes. Moreover, the expression of PtToll4 could be moderately and shortly upregulated by Vibrio alginolyticus or lipopolysaccharide challenge in the primary cultured hemocytes. In HEK 293T cell model, over‐expressed PtToll4 was not able to activate the mammal NF‐κB, compared with a positive plasmid. In the Drosophila S2 cell model, over‐expressed PtToll4 distributed mainly in cell membrane and could affect the activation of some Drosophila antimicrobial peptides promoters. The RNA interference experiment showed that the expressions of PtToll4 was significantly inhibited by its specific double‐stranded RNA in the crab hemocytes and accompanied by the suppressed expressions of the anti‐lipopolysaccharide factor 4 (ALF4), ALF7, hyastatin3, crustin1, and crustin3.
Morphology and Reproductive Biology of Blue Perch Badis assamensis Ahl 1937 Recorded From a Hill Stream in Assam, India
Authors: Hussain Jafrin Farha , Bordoloi Sabitry 
Published: January 2025

ABSTRACTA badid, Badis assamensis Ahl, 1937, was recorded for the first time from the intermediate zone of a semi‐torrential stream called Basistha in Assam, which is a small tributary of the river Brahmaputra. The fish species Badis assamensis is currently listed in the data deficient (DD) category of IUCN. The species has previously been reported in swampy wetlands in the upper reaches of the Brahmaputra valley in Assam; with no detail description on its biology however. The current work redescribes morphology and presents detail reproductive biology of this species based on fresh collection of 377 individuals from the Basistha. length‐weight relationships (LWR) analysed in this species indicated an isometric growth; and condition factor (K) indicated a suitable habitat for this species. Badis assamensis exhibits a non‐seasonal breeding behaviour twice a year, as analysed from the Gonado‐somatic and ovarian indices. During certain period of the year, local people use unscientific ways of fish collection thereby resulting in a steep decline in fish population. The study is believed to lay an insight into this species' geographical range from a swampy wetland habitat in upper reaches of the Brahmaputra basin; to a semi‐torrential hill stream habitat in the lower Brahmaputra sub‐basin; thereby providing baseline information on this DD species. Analysis of morphometric and reproductive parameters of this species not reported earlier is the major highlight of the present study.
Predicting Elevated Postvoid Residual Urine Volume Following OnabotulinumtoxinA Treatment for Overactive Bladder: A Pilot Study
Authors: Franco Israel , Schwartz Marc , Cline Kevin , Glazier David , Patel Anand 
Published: January 2025

ABSTRACTObjectivesTo evaluate possible predictors of elevated postvoid residual volume (PVR) following onabotulinumtoxinA administration in patients with idiopathic overactive bladder (OAB), a condition that may include urinary urgency, frequency, and nocturia, without any identifiable cause or underlying neurological or metabolic condition.MethodsAdults who had been treated with 100–200 U onabotulinumtoxinA for OAB and had previous failure of other OAB treatments were identified by retrospective review of medical chart data from three urology clinics in the United States treating patients with a variety of urological conditions. A total of 211 patients were allocated to cohorts based on posttreatment PVR < 200 mL (n = 173) and ≥ 200 mL (n = 38). Logistic regression analyses were performed to evaluate potential predictors of posttreatment PVR ≥ 200 mL, including pretreatment peak urine flow rate (Qmax), average urine flow rate (Qavg), and Modified Liverpool Qmax and Qavg flow index (FI), and to determine whether patient age and baseline PVR were associated with the likelihood of PVR ≥ 200 mL. Patients were excluded if symptoms of OAB were secondary to a neurological condition, they had a PVR > 200 mL within 2 weeks prior to the index therapy or had been treated with other botulinum toxin formulations for a urinary condition.ResultsIn the predictor analyses, neither Qmax nor Qavg alone was a likely predictor. Odds ratios of PVR ≥ 200 mL for Modified Liverpool Qmax FI and Qavg were 0.30 (95% CI: 0.08–0.91; p = 0.0488) and 0.07 (95% CI 0.01–0.40; p = 0.0045), respectively. When patient age and baseline PVR were incorporated into the analyses, results suggested that Qmax, Qavg, Qmax FI, and Qavg FI, as well as increased age and baseline PVR, were likely predictors of elevated posttreatment PVR.ConclusionsPatients who are older, have high pretreatment PVR values, and have lower pretreatment urine flow indexes and flows may be at increased risk of developing elevated PVR after receiving onabotulinumtoxinA treatment for OAB.
From division to ‘divergence’: to understand wood growth across timescales, we need to (learn to) manipulate it
Authors: Buttò Valentina , Peltier Drew M. P. , Rademacher Tim 
Published: January 2025

SummaryWood formation is the Rosetta stone of tree physiology: a traceable, integrated record of physiological and morphological status. It also produces a large and persistent annual sink for terrestrial carbon, motivating predictive understanding. Xylogenesis studies have greatly expanded our knowledge of the intra‐annual controls on wood formation, while dendroecology has quantified the environmental drivers of multi‐annual variability. But these fields operate on different timescales, making it challenging to predict how short (e.g. turgor) and long timescale processes (e.g. disturbance) interactively influence wood formation. Toward this challenge, wood growth responses to natural climate events provide useful but incomplete explanations of tree growth variability. By contrast, direct manipulations of the tree vascular system have yielded unexpected insights, particularly outside of model species like boreal conifers, but they remain underutilized. To improve prediction of global wood formation, we argue for a new generation of experimental manipulations of wood growth across seasons, species, and ecosystems. Such manipulations should expand inference to diverse forests and capture inter‐ and intra‐specific differences in wood growth. We summarize the endogenous and exogenous factors influencing wood formation to guide future experimental design and hypotheses. We highlight key opportunities for manipulative studies integrating measurements from xylogenesis, dendroanatomy, dendroecology, and ecophysiology.
The Reliability and Validity of Intraoral Photographs in Assessing Orthodontic Treatment Need
Authors: Göranson Emma , Sonesson Mikael , Gullbrand Malin , Isberg Per‐Erik , Dimberg Lillemor 
Published: January 2025

ABSTRACTBackgroundOrthodontic treatment need has commonly been assessed using treatment need indices during clinical examinations or using photographs in combination with plaster casts. Recently, the use of intraoral photographs alone to screen malocclusions has increased.ObjectiveThis study aimed to validate intraoral photographs for the assessment of orthodontic treatment need.Materials and MethodsThe study sample consisted of case files from 30 pre‐orthodontic patients aged 12–19 years. Each case file included intraoral photographs and casts. The cases were consecutively recruited from two orthodontic treatment waiting lists: 15 from the Department of Orthodontics at Folktandvården Eastmaninstitutet, Stockholm, Sweden and 15 from the Center for Orthodontics and Pediatric Dentistry, Norrköping, Public Dental Service Östergötland. Their orthodontic treatment need was assessed by four raters (calibrated orthodontists) using the indices IOTN‐AC, IOTN‐DHC, ICON, and DAI. The four raters individually assessed the 30 cases on three occasions: (1) photos only, (2) photos and casts, and (3) photos only. Finally, the four raters jointly made a consensus assessment using both photos and casts. For IOTN‐AC and IOTN‐DHC, interrater agreement was assessed with Fleiss' kappa, and validity with the Wilcoxon signed‐rank test. For ICON and DAI, interrater agreement was assessed with Intra Class Correlation (ICC) (1, 2) and validity with a paired t‐test.ResultsInterrater agreement for IOTN‐AC was slight (0.14–0.18) and moderate for IOTN‐DHC (0.51–0.57), regardless of whether photographs were used alone or combined with casts. ICON demonstrated moderate interrater agreement (0.57–0.72), while DAI showed moderate to excellent (0.70–0.91), similarly unaffected by the use of photographs alone or in combination with casts. The validity analysis, which compared the individual assessments to the consensus one, revealed equivalent differences whether the assessment involved intraoral photographs alone or in combination with casts.ConclusionIntraoral photographs are sufficient for assessing orthodontic treatment need because interrater agreement and validity are similar whether photos are used alone or with casts. However, interrater variability was substantial for both assessment methods.Trial RegistrationClinicalTrial.gov. identifier: NCT05038865