bluuebunny/crossref_metadata_2025_split huggingface dataset:
The welfare impact of gavaging laboratory rats
Authors: Bonnichsen M , Dragsted N , Hansen AK 
Published: January 2023

AbstractGavaging (oral dosing) has previously been shown to have only a short-term effect on behavioural parameters in the laboratory rat. The aim of this study was to determine if the gavaging of laboratory rats influenced their heart rate, blood pressure and body temperature, and if so, whether the duration of this impact correlated with the volume gavaged. The three stress parameters were measured using telemetric transponders placed in the abdomen of eight female Sprague-Dawley (Mol:SPRD) rats. Using a Latin Square cross-over design, the rats were gavaged with three different doses of barium sulphate (4, 10 and 40 ml kg–1); in addition, there was a control of no dose, only insertion of the tube. The heart rate, blood pressure and body temperature of the rats were monitored continuously for 4 h after dosing and again for 1 h, 24 h after dosing. The gavaging of laboratory rats was shown to induce an acute reaction: after 30 min, blood pressure and heart rate were significantly higher than before gavaging, and body temperature was significantly higher 60 min after gavaging — indicators of stress levels comparable to those of other basic experimental procedures. A significant correlation between heart rate and dosage was observed until 10 min after gavaging. This indicates that the dosage gavaged is of only minor importance in causing stress, and only important for the most acute reaction. However, because of the resistance and discomfort observed when administering a 40 ml kg–1dose, this dose should be administered only with caution.
Housing environment alters delayed-type hypersensitivity and corticosterone concentrations of individually housed male C57BL/6 mice
Authors: Neigh GN , Bowers SL , Korman B , Nelson RJ 
Published: January 2023

AbstractHousing conditions can alter both the physiology and behaviour of laboratory animals. Forced-air-ventilated micro-isolation systems increase the efficient use of space, decrease the incidence of disease among laboratory rodents, and provide better working conditions for animal care staff; however, such systems can increase breeding variability and mortality. We examined the possibility that stressors associated with automated housing conditions evoke subtle changes among immune, endocrine, and behavioural parameters in mice housed in a static versus a forced-air-ventilated micro-isolation system. In addition, we assessed the effects of housing in the forced-air-ventilated micro-isolation system both with and without the use of an automatic watering system. Housing in the forced-air-ventilated micro-isolation system, using the automatic watering system, suppressed delayed-type hypersensitivity (DTH) responses, a measure of cell mediated immune function, compared with the responses of mice housed in static cages. Hypothalamic–pituitary–adrenal axis function was also altered by housing in the forced-air-ventilated micro-isolation system with the use of the automatic watering system, such that mice in this housing system had lower resting corticosterone concentrations and increased reactivity to restraint. Despite these changes in corticosterone, housing condition did not alter activity level or exploratory, anxiety-like, or depressive-like behaviours. These results suggest that investigators should carefully consider housing conditions in studies of immune and endocrine function.
Effective searching of the scientific literature for alternatives: search grids for appropriate databases
Authors: Hart LA , Wood MW , Weng H-Y 
Published: January 2023

AbstractResearchers searching for alternatives to painful procedures that involve animals may find that the dispersed relevant literature and the array of databases make the search challenging and even onerous. This paper addresses a significant gap that exists for researchers, in identifying appropriate databases to use when searching for specific types of information on alternatives. To facilitate the efficient and effective searching by users, and to ensure compliance with new requirements and improved science, we initiate an evolving guide comprising search grids of database resources organised by animal models and topics (http://www.vetmed.ucdavis.edu/Animal_Alternatives/databaseapproach.html). The search grids present organised lists of specific databases and other resources for each animal model and topic, with live links. The search grids also indicate resources that are freely available worldwide, and those that are proprietary and available only to subscribers. The search grids are divided into two categories: ‘animal models’ and ‘topics’. The category ‘animal models’ comprises: animal model selections; mice; rodents — rats/guinea pigs/hamsters; rabbits; dogs, cats; farm animals, sheep, swine; non-human primates; fish, frogs, aquatic; and exotics. The category ‘topics’ comprises: husbandry; behaviour; euthanasia; toxicity; monoclonal antibodies; teaching; endpoints; disease models; analgesia/anaesthesia; emerging technologies; strategies for specific intervention procedures; and standard operating procedures (for example, drawing blood, behavioural training, transportation, handling, restraint and identification). Users are provided with a selected list of linked resources relevant to their particular search. Starting with an appropriate database that covers the type of information that is being sought is the first step in conducting an effective search that can yield useful information to enhance animal welfare.
The application of Russell and Burch's Three Rs in commercial livestock experimentation
Authors: Colditz IG 
Published: January 2023

AbstractThe inclusion of Russell and Burch's Three Rs (replacement, reduction and refinement) in guidelines, codes of practice and law reflects their current position as the guiding principles of ethical assessment of research involving animals. This article explores some activities within the contemporary livestock industry that constitute the experimental use of animals on a local and global scale. The elucidation of correlated responses during trait selection in genetic improvement programs provides one example of experiments occurring within the commercial livestock industry. This experimentation is largely conducted without scrutiny of its conformity to the Three Rs. Experimentation to improve the management of the livestock industry is consistent with the principle of refinement, and experimentation to increase productivity per unit of livestock is consistent with the principle of reduction; however, experimentation to increase total livestock production conflicts with the principle of replacement. Some approaches regarding the appraisal of the ethics of research involving animals, which could avoid arbitrary boundaries associated with the location or purpose of experimentation, are considered together with the relationship between experimentation and other anthropogenic impacts on animals.
Motivation for group housing in gestating sows
Authors: Kirkden RD , Pajor A 
Published: January 2023

AbstractIt has been argued that the welfare of gestating sows is higher in groups than singly in stalls, in part because group housing offers them more space and social contact. This study set out to ascertain how important access to a group pen was to dominant sows housed in stalls, using a measure of motivation. Subjects were trained to perform a panel-pressing task, then housed in a stall and permitted each day to work for a day's access to a fully slatted group pen containing two familiar, subordinate sows at a stocking density of 2.7m2per pig. Social ranking was determined by observations at mixing and from feed competition tests. The fixed-ratio schedule was increased daily and the highest schedule reached (the reservation price) was used as a measure of motivational strength. To interpret this measure, it was compared with the highest schedule that subjects reached when working for access to the last 1/16thof their estimatedad libitumdaily food intake after having consumed the first 15/16thsfree. Sixteen subjects were tested, eight working for access to the group pen first and eight for access to the food first. Seven subjects yielded useable data: four reached a higher schedule working for food and three reached a higher schedule working for the group pen. Overall, subjects attached no more importance to a day's access to the group pen than to the last 1/16thof their estimatedad libitumfood intake. It is likely that the subjects were close to satiation when working for food because consumption frequently fell substantially short of the ‘ad libitum‘ allowance. These results suggest that dominant, stall-housed sows are only weakly motivated to gain access to a fully slatted group pen, although motivation might be higher when deprived of access to the group pen for longer than one day, if tested at a different time of day or if the quality of the group space was improved; these three possibilities still need to be tested.
Can non-invasive glucocorticoid measures be used as reliable indicators of stress in animals?
Authors: Lane J 
Published: January 2023

AbstractRefinement techniques are being increasingly employed in all fields of animal research to try to ensure that the highest standards of welfare are upheld. This review concerns one of the main emerging techniques for the assessment of welfare itself, namely the non-invasive measurement of glucocorticoids (GCs) as indicators of stress. The paper is divided into three sections. The first discusses the relationship between GCs and stress. The second section considers whether factors other than stress are linked to rises in GCs, eg exercise, oestrus cycle and diet. The final part examines the reliability of the non-invasive techniques that measure GCs from samples of saliva and faeces. Although it is important to take into account some caveats associated with the methodologies employed, it is concluded, nevertheless, that these techniques can give accurate and reliable information regarding the welfare status of an individual or group of animals without the procedures themselves causing any kind of distress to the subjects.
Song development in birds: the role of early experience and its potential effect on rehabilitation success
Authors: Spencer KA , Harris S , Baker PJ , Cuthill IC 
Published: January 2023

AbstractEnvironmental conditions during the early life stages of birds can have significant effects on the quality of sexual signals in adulthood, especially song, and these ultimately have consequences for breeding success and fitness. This has wide-ranging implications for the rehabilitation protocols undertaken in wildlife hospitals which aim to return captive-reared animals to their natural habitat. Here we review the current literature on bird song development and learning in order to determine the potential impact that the rearing of juvenile songbirds in captivity can have on rehabilitation success. We quantify the effects of reduced learning on song structure and relate this to the possible effects on an individual's ability to defend a territory or attract a mate. We show the importance of providing a conspecific auditory model for birds to learn from in the early stages post-fledging, either via live- or tape-tutoring and provide suggestions for tutoring regimes. We also highlight the historical focus on learning in a few model species that has left an information gap in our knowledge for most species reared at wildlife hospitals.
Validation of broiler chicken gait scoring training in Thailand, Brazil and New Zealand
Authors: Butterworth A , Knowles TG , Whittington P , Matthews L , Rogers A , Bagshaw CS 
Published: January 2023

AbstractBroiler chickens are the most numerous terrestrial farmed animals on earth. These birds can suffer a range of pathologies which result in lameness, and one technique for assessing the impact of lameness on the individual bird, and on the flock has been gait scoring. This paper describes a study where the results of the same training programme for validated gait scoring are compared for groups of assessors in Thailand, Brazil and New Zealand. A uniform course of training carried out in the classroom, through video assessment and through on-farm training was carried out in the countries named; a total of 95 persons being trained overall. A measure of agreement with the reference scale for the assessors' numerical responses was carried out using a Cohen's Kappa test, and the results indicated significant differences in the ‘origin position’ ie the view taken by the assessors of the impact of lameness on the bird before training had taken place, but a high degree of consistency in the response to training. The study demonstrated that gait scoring can be learned by assessors from different geographical and social backgrounds, and that the ‘rate of learning’ – the improvement in performance in relation to a standard, was similar between the countries studied. The ‘origin’ and ‘final’ position of the assessors pre- and post-training, from the different countries in relation to the reference standard is discussed.
Testing different methods to evaluate pig welfare on farm
Authors: Courboulay V , Foubert C 
Published: January 2023

AbstractFeasibility and validity of protocols used to assess welfare were evaluated in two fattening rooms in twenty farms, chosen according to the group size, pen shape, floor and space per animal. The two rooms contained animals at the beginning or at the end of the fattening period, respectively. Behaviour was recorded either through direct observations of the pens (3 times per minute, at 5 min intervals), 15 minutes after entering the room (B1) or after the removal of an object given at the end of the visit (B3). The third protocol consisted of a 2 minute observation inside the pen (B2). To evaluate the human-animal interaction, the time to adapt to the presence of the operator in the corridor (HA1) was measured, as well as the reaction of the group when the operator entered the pen (HA2) or walked slowly through the pen (HA3), the time taken by the first five pigs to approach the operator (HA4) and the reaction of the animals when the operator tried to catch an ear (HA5). Lesion scoring was carried out inside the pen on a sample of 60 individual fatteners or at pen level on most of the pigs from the corridor. The occurrence of the main active behaviours is assessed similarly over time by B1and B3. Behavioural observations are then possible in an on-farm welfare assessment. Lesion scoring from the corridor tends to under estimate the number of lesions (scratches, tail wounds). The HA3test appears to be the best one to evaluate the relationship towards humans but has to be validated.
Results of the Animal Needs Index (ANI-35L) compared to animal-based parameters in free-range and organic laying hen flocks in Austria
Authors: Zaludik K , Lugmair A , Baumung R , Troxler J , Niebuhr K 
Published: January 2023

AbstractThe aim of this study was a comparison of Animal Needs Index (ANI) data, derived from annual inspections by a control agency, with data collected from 164 selected on-farm flocks concerning feather damage, injuries, egg production, mortality, bodyweight, foot pad dermatitis, keel bone deviations and reactions towards humans (eg flock showed marked avoidance when the observer walked through the hen-house [yes/no]).Analysis of data showed a low number of significant correlations with total ANI scores and category scores: 1) Locomotion, 2) Social interaction, 3) Flooring, 4) Light, air and noise and 5) Stockmanship. Correlations found were low and total ANI score showed only a positive correlation with egg production at week 52. Category 3 scores correlated positively with egg production at week 70 and negatively with the percentage of hens with featherless areas and total pecking injuries. Category 5 scores showed positive correlations with egg production at week 52 as well as week 70 and negative correlations with mortality at week 52, the percentage of hens with featherless areas, pecking injuries < 0.5 cm and total pecking injuries.Flocks showing marked avoidance had a lower total ANI score and lower category 3 and category 5 scores.In conclusion, welfare-related animal-based parameters are poorly reflected by the ANI-35-L/2001. To assess animal welfare more adequately, animal-based parameters have to be considered additionally in a welfare assessment scheme.
Formal animal-based welfare assessment in UK certification schemes
Authors: Main DCJ , Whay HR , Leeb C , Webster AJF 
Published: January 2023

AbstractProviding assurance of improvement in welfare outcomes is the desire of some UK certification schemes. However, this is only likely to be achieved if such schemes monitor welfare outcomes rather than rely on defining welfare resources that should be provided. The University of Bristol has developed protocols for the on-farm evaluation of animal-based welfare parameters for incorporation into certification schemes. The assessment system is designed to provide credible (repeatable, valid and feasible) evidence for assessment of compliance with welfare standards in organic and conventional farming systems. For issues identified as causing potential concern the assessor is encouraged to conduct further investigations of the relevant resource standards and, where appropriate, management requirements. Furthermore the assessment tool enables certification bodies and relevant third parties to monitor the ability of schemes to deliver good welfare outcomes. Finally, it provides a mechanism for farmers to review their own health and welfare performance. The practical application of these systems is now being pursued by some UK certification bodies.
Self-evaluation of animal welfare by the farmer: a report of application on Austrian cattle farms
Authors: Ofner E , Schmid E , Schröck E , Troxler J , Hausleitner A 
Published: January 2023

AbstractIt is not sufficient to merely publish regulations and strictly control them to improve the living conditions of farm animals. The farmer must receive precise information, understand the individual measures and be able to act with personal responsibility. A new animal welfare law has been in force in Austria since the 1st of January 2005. A system of self-evaluation is being developed for putting these new legal requirements into practice. The aim of this self-evaluation system is to enable every farmer to personally check his animal housing system against the given standards. This independent work motivates the farmer and makes him aware of animal welfare issues. This new strategy for the implementation of animal welfare law was originally developed, tested and found to be successful by the Federal Administration of the Austrian Province of Vorarlberg. Self-evaluation is to be carried out by means of specific checklists and manuals. Checklists and manuals are currently being drawn up for cattle, pigs, poultry, sheep and goats. The checklists and manuals will include all of the requirements of Austrian animal welfare legislation and will be formulated in easily understandable questions with ‘yes’ or ‘no’ answers. The manuals will describe the methods for assessing legal requirements on the farm as well as the conditions for compliance with legislation, and give advice on how to improve the state of animal welfare on the farm. This paper gives an example of the self-evaluation system for cattle farming.
Bridging consumer perception and on-farm assessment of animal welfare
Authors: Napolitano F , De Rosa G , Caporale G , Carlucci A , Grasso F , Monteleone E 
Published: January 2023

AbstractThe present study aims to assess the relationship between consumer perception and on-farm assessment of animal welfare performed using the Animal Needs Index 35L (ANI). Two tie-stall, 2 straw yard and 2 cubicle farms were scored by trained assessors using the ANI and filmed to produce six 3.5 min videos. Each of them contained 4 clips: barn overall view, feeding, milking and individual animals. Ten untrained observers were asked to elicit terms describing how they perceived the observed farming systems to affect animal welfare using Free Choice Profiling (FCP). Data from FCP were subjected to Generalised Procrustes Analysis (GPA). GPA showed a significant consensus among observers. Observers characterised the first dimension with terms ranging from constrictive to comfortable conditions and the second one in terms of cleanliness/dirtiness. Principal Component Analysis (PCA) was conducted using the scores of the farms on the first two dimensions of GPA and the data gathered through the ANI. The first dimension of GPA (Comfortable), ANI's sheet 1 (Locomotion), sheet 2 (Social interaction) and sheet 6 (Total score) showed high loadings on the first component of PCA, whereas the second dimension of GPA (Clean), sheet 3 (Flooring) and sheet 4 (Stockmanship) were correlated with the second component of PCA. We concluded that FCP may be used to elicit lay person perception of welfare-related characteristics of dairy cattle farming systems, thus providing a tool to study the relationships between consumer perception and on-farm assessment of animal welfare.
Using the Piglet Scream Test to enhance piglet survival on farms: data from outdoor sows
Authors: Held S , Mason G , Mendl M 
Published: January 2023

AbstractThe point of the Piglet Scream Test is to identify those sows that increase the survival of their piglets through good maternal behaviour. For this to work, sows must be shown to differ in their test responsiveness, for differences to be consistent across parities and for responsiveness to be associated with piglet survival. Our data provide some first evidence that outdoor sows, kept under commercial production conditions, differ consistently from each other in their test responsiveness across parities. However, there was no evidence that this was associated with the survival of their litters. This, together with the lack of consistent evidence from other studies for a relationship between Piglet Scream Test responsiveness and piglet survival and concerns about its possible causes, cautions against the use of the test to enhance piglet survival on farms without further study. The paper highlights lack of standardisation of the test and pseudoreplication as concerns.
Anthropomorphism and anthropocentrism as influences in the quality of life of companion animals
Authors: Bradshaw JWS , Casey RA 
Published: January 2023

AbstractSince animal minds are private, so their perception of their own quality of life (QoL) must be also. Anthropocentrism, the interpretation of reality exclusively in terms of human values and experience, has to be guarded against in any assessment of animal welfare; for domestic pets, misapprehensions about their olfactory and cognitive abilities appear to present the greatest challenge to their welfare. Anthropomorphism, the attribution of human qualities to animals, presents a particular problem when considering companion animals, since most bonds between owners and their pets appear to be based upon a perception of the pet as almost human. Many owners report that their dogs, cats and horses are capable of feeling complex emotions, such as pride and guilt, that require a level of self-awareness that has been difficult to demonstrate even in chimpanzees. Such beliefs appear to contribute to the development of behavioural disorders in pets; for example, clinical experience suggests that the application of punishment by owners who attribute ‘guilt’ to their animals may unwittingly lead to compromised welfare. Anthropomorphic owners are also likely to be poor proxies for reporting their pets' QoL.