bluuebunny/crossref_metadata_2025_split huggingface dataset:
A regulatory circuit involving the NADH dehydrogenase-like complex balances C4photosynthetic carbon flow and cellular redox in maize
Authors: Zhang Qiqi , Tian Shilong , Chen Genyun , Tang Qiming , Zhang Yijing , Fleming Andrew J. , Zhu Xin-Guang , Wang Peng 
Published: February 2023

SummaryC4plants typically operate a CO2concentration mechanism from mesophyll (M) cells into bundle sheath (BS) cells. NADH dehydrogenase-like (NDH) complex is enriched in the BS cells of many NADP-ME type C4plants, and is more abundant in C4than in C3plants, but to what extent it is involved in the CO2concentration mechanism remains to be experimentally investigated.We created maize and rice mutants deficient in NDH function, and used a combination of transcriptomic, proteomic, and metabolomic approaches for comparative analysis.Considerable decrease in growth, photosynthetic activities, and levels of key photosynthetic proteins were observed in maize but not rice mutants. However, gene expression for many cyclic electron transport and Calvin-Benson cycle components plus BS specific C4enzymes, was up-regulated in maize mutants. Metabolite analysis of the maizendhmutants revealed increased NADPH/NADP ratio, as well as malate, RuBP, FBP, and photorespiration components.We suggest that by optimizing NADPH and malate levels, adjusting NADP-ME activity, NDH functions to balance metabolic and redox states in the BS cells of maize, coordinating photosynthetic gene expression and protein content, thus directly regulating the carbon flow in the two-celled C4system of maize.
Dying cell-released exosomal CXCL1 promotes breast cancer metastasis by activating TAM/PD-L1 signaling
Authors: Wang Shengqi , Li Jing , Wang Neng , Yang Bowen , Zheng Yifeng , Wang Xuan , Zhang Juping , Pan Bo , Wang Zhiyu 
Published: February 2023

AbstractBackgroundEmerging evidence suggests that dying cell-released signals may induce cancer progression and metastasis by modulating the surrounding microenvironment. However, the underlying molecular mechanisms and targeting strategies are yet to be explored.MethodsApoptotic breast cancer cells induced by paclitaxel treatment were sorted and their released exosomes (exo-dead) were isolated from the cell supernatants. Chemokine array analysis was conducted to identify the crucial molecules in exo-dead. Zebrafish and mouse xenograft models were used to investigate the effect of exo-dead on breast cancer progressionin vivo. Multiple molecular biological experiments were conducted to determine the underlying mechanisms of exo-dead in promoting breast cancer, as well as its intervention values.ResultsIt was demonstrated that exo-dead were phagocytized by macrophages and induced breast cancer metastasis by promoting the infiltration of immunosuppressive PD-L1+TAMs. Chemokine array identified CXCL1 as a crucial component in exo-dead to activate TAM/PD-L1 signaling. Exosomal CXCL1 knockdown or macrophage depletion significantly inhibited exo-dead-induced breast cancer growth and metastasis. Mechanistic investigations revealed that CXCL1exo-deadenhanced TAM/PD-L1 signaling by transcriptionally activating EED-mediated PD-L1 promoter activity. More importantly, TPCA-1 (2-[(aminocarbonyl) amino]-5-(4-fluorophenyl)-3-thiophenecarboxamide) was screened as a promising inhibitor targeting exosomal CXCL1 signals to enhance paclitaxel chemosensitivity and limit breast cancer metastasis without noticeable toxicities.ConclusionsOur results highlight CXCL1exo-deadas a novel dying cell-released signal and provide TPCA-1 as a targeting candidate to improve breast cancer prognosis.
Effects of ploidy and genetic diversity on competitive outcomes
Authors: Pérez-Romero Jesús Alberto , Muñoz Ana García , Olivieri Enrica , Muñoz-Pajares A. Jesús , Abdelaziz Mohamed , Godoy Oscar 
Published: February 2023

SummaryGenetic diversity affects evolutionary trajectories but their ultimate effects on ecological interactions and community dynamics remains poorly understood. It has been hypothesized that phenotypic novelties produced by ploidy and heterozygosity modify the ecological interactions between novel genotypes and more ancient locally adapted ones, and therefore, their opportunities to coexist.We performed a greenhouse competition experiment with three taxa of theErysimum incanumspecies complex differing in ploidy (2x, 4x and 6x) and heterozygosity (high and low). This experiment allows us to parameterize a population model to test the effect of genetic diversity on modulating the ecological forces that determine the outcome of competition, niche and fitness differences.Depending on whether ploidy variation and the level of heterozygosity made interspecific competition greater or smaller than intraspecific competition, we predicted either priority effects or coexistence. Such competitive outcome differences were explained by the phenotypic expression in the number of stalks (plant size surrogate) with genotypes under priority effects showing more stalks.Altogether, our results show that non-polyploid plants can coexist with polyploids contravening theoretical expectations of polyploidy dominance under stable conditions. However, historical contingency such as order of arrival promotes priority effects when adaptive phenotypic optimums strongly compete for space.
Polygenic dynamics underlying the response of quantitative traits to directional selection
Authors: Götsch Hannah , Bürger Reinhard 
Published: February 2023

AbstractWe study the response of a quantitative trait to exponential directional selection in a finite haploid population, both at the genetic and the phenotypic level. We assume an infinite sites model, in which the number of new mutations per generation in the population follows a Poisson distribution (with mean Θ) and each mutation occurs at a new, previously monomorphic site. Mutation effects are beneficial and drawn from a distribution. Sites are unlinked and contribute additively to the trait. Assuming that selection is stronger than random genetic drift, we model the initial phase of the dynamics by a supercritical Galton-Watson process. This enables us to obtain time-dependent results. We show that the copy-number distribution of the mutant in generationn, conditioned on non-extinction untiln, is described accurately by the deterministic increase from an initial distribution with mean 1. This distribution is related to the absolutely continuous partW+of the random variable, typically denotedW, that characterizes the stochasticity accumulating during the mutant’s sweep. A suitable transformation yields the approximate dynamics of the mutant frequency distribution in a Wright-Fisher population of sizeN. Our expression provides a very accurate approximation except when mutant frequencies are close to 1. On this basis, we derive explicitly the (approximate) time dependence of the expected mean and variance of the trait and of the expected number of segregating sites. Unexpectedly, we obtain highly accurate approximations for all times, even for the quasi-stationary phase when the expected per-generation response and the trait variance have equilibrated. The latter refine classical results. In addition, we find that Θ is the main determinant of the pattern of adaptation at the genetic level, i.e., whether the initial allele-frequency dynamics are best described by sweep-like patterns at few loci or small allele-frequency shifts at many. The number of segregating sites is an appropriate indicator for these patterns. The selection strength determines primarily the rate of adaptation. The accuracy of our results is tested by comprehensive simulations in a Wright-Fisher framework. We argue that our results apply to more complex forms of directional selection.
MicroRNA-195-3p as a potential regulator of hypoxic injury in HUVECs
Authors: Zhang Wenjing , Liu Bingshi , Wang Yanfang , Sun Lixian , Liu Chao , Zhang Haoran , Qin Wei , Liu Jingyi , Han Leng , Cui Zhoukai , Shan Weichao 
Published: February 2023

ABSTRACTCoronary heart disease is the most common heart disease and is the leading cause of cardiovascular death worldwide. Revascularization methods are considered effective against coronary heart disease However, the mechanism of molecular revascularization remains largely unknown. Endothelial cells are the primary cells that initiate angiogenesis and arteriogenesis and require a hypoxic environment for induction. In this study, we aimed to determine the expression and role of microRNA-195-3p in hypoxia-treated HUVEs(human umbilical vein endothelial cells). Herein, we induced hypoxia in human umbilical vein endothelial cells using the “Anaerobic tank method.” Hypoxia injured human umbilical vein endothelial cells showed upregulation of microRNA-195-3p; decreased cell proliferation, migration, and autophagy; and increased apoptosis. Furthermore, the microRNA-195-3p inhibitor partially reversed the effects of hypoxia-induced injury of human umbilical vein endothelial cells. Therapeutic intervention using microRNA-195-3p inhibitor could maintain endothelial cell function under hypoxic conditions, improve cell activity, and be considered a new treatment strategy for coronary heart diseases.
Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus valley and South Indian origin
Authors: Lokugalappatti LGS , Wickramasinghe Saumya , Alexander P.A.B.D , Abbas Kamran , Hussain Tanveer , Ramasamy Saravanan , Manomohan Vandana , Tapsoba Arnaud Stephane R. , Pichler Rudolf , Babar Masroor E. , Periasamy Kathiravan 
Published: February 2023

AbstractThe present study reports the population structure, genetic admixture and phylogeography of island cattle breeds of Sri Lanka viz. Batu Harak, Thawalam and White cattle. Moderately high level of genetic diversity was observed. Estimates of inbreeding for Thawalam and White cattle breeds were relatively high with 6.1% and 7.2% respectively. Genetic differentiation of Sri Lankan Zebu (Batu Harak and White cattle) was lowest with Red Sindhi among Indus valley zebu while it was lowest with Hallikar among the South Indian cattle. Global F statistics showed 6.5% differences among all the investigated zebu cattle breeds and 1.9% differences among Sri Lankan zebu breeds. The Sri Lankan zebu cattle breeds showed strong genetic relationships with Hallikar cattle, an ancient breed considered to be ancestor for most Mysore type draught cattle breeds of South India. Genetic admixture analysis revealed high levels of breed purity in Lanka White cattle with >97% zebu ancestry while significant taurine admixture was observed in Batu Harak and Thawalam cattle. Two major zebu haplogroups, I1 and I2 were observed in Sri Lankan zebu with the former predominating the later in all the three breeds. A total of 112 haplotypes were observed in the studied breeds, of which 50 haplotypes were found in Sri Lankan zebu cattle. Mismatch analysis revealed unimodal distribution in all the three breeds indicating population expansion. The sum of squared deviations (SSD) and raggedness index were non-significant in both the lineages of all the three breeds except for I1 lineage of Thawalam cattle (P<0.01) and I2 lineage of Batu Harak cattle (P<0.05). The results of neutrality tests revealed negative Tajima’s D values for both the lineages of Batu Harak (P>0.05) and White cattle (P>0.05) indicating an excess of low frequency polymorphisms and demographic expansion. Genetic dilution of native zebu cattle germplasm is a cause for concern and it is imperative that national breeding organizations consider establishing conservation units for the three native cattle breeds to maintain breed purity and initiate genetic improvement programs.
Indoor and outdoor air pollutants as emerging public health threat in Latin America and the Caribbean: a systematic review
Authors: Husaini Danladi Chiroma , Manzur Kemberly , Medrano Jorge 
Published: February 2023

PurposeThis systematic review examined the emerging threat of indoor and outdoor pollutants to public health in Latin America and the Caribbean (LAC).Design/methodology/approachPollutants and pollution levels are becoming an increasing cause for concern within the LAC region, primarily because of the rapid increase in urbanization and the use of fossil fuels. The rise in indoor and outdoor air pollutants impacts public health, and there are limited regional studies on the impact of these pollutants and how they affect public health. A comprehensive literature search was conducted using Google Scholar, PubMed, Scopus, EBSCOhost, Web of Science and ScienceDirect databases. Significant search terms included “indoor air pollution,” “outdoor air pollution,” “pollution,” “Latin America,” “Central America,” “South America” and “Caribbean was used.” The systematic review utilized the Rayyan systematic software for uploading and sorting study references.FindingsDatabase searches produced 1,674 results, of which, after using the inclusion–exclusion criteria and assessing for bias, 16 studies were included and used for the systematic review. These studies covered both indoor and outdoor pollution. Various indoor and outdoor air pollutants linked to low birth weight, asthma, cancer and DNA impairment were reported in this review. Even though only some intervention programs are available within the region to mitigate the harmful effects of pollution, these programs need to be robust and appropriately implemented, causing possible threats to public health. Significant gaps in the research were identified, especially in the Caribbean.Research limitations/implicationsLimitations of the study include limited available research done within LAC, with most of the research quantifying pollutants rather than addressing their impacts. Additionally, most studies focus on air pollution but neglect water and land pollution’s effects on public health. For this reason, the 16 studies included limited robustness of the review.Originality/valueAlthough available studies quantifying pollution threats in LAC were identified in this review, research on the adverse impacts of pollution, especially concerning public health, is limited. LAC countries should explore making cities more energy-efficient, compact and green while improving the transportation sector by utilizing clean power generation. In order to properly lessen the effects of pollution on public health, more research needs to be done and implemented programs that are working need to be strengthened and expanded.
Multicultural conflict cultures: more similarities than differences in diverse societies
Authors: Desrayaud Nathalie , Hurley Carolyn M. 
Published: February 2023

Purpose
This study aims to understand how cultural variables – collectivism and face concerns – influence perceived and ideal organizational conflict cultures in two multicultural societies.

Design/methodology/approach
Individuals studying in Singapore (N = 162) and the USA (N = 216) completed an online survey. Hierarchical regression analyses tested the hypotheses.

Findings
Regardless of cultural background, individuals prefer agreeable conflict cultures, with over 95% scoring above the scale mid-point. Ideal passiveness varied; highly collective and mutual face concerned individuals were more likely to idealize active conflict cultures. Collectivism overall was associated with perceiving and idealizing active and agreeable conflict cultures, though some relationships were only significant for one sample. Self-face concerned individuals were more likely to perceive passive conflict cultures.

Research limitations/implications
Culture influences organizational conflict cultures, but not necessarily in stereotypical ways. Despite cultural and geographical differences, the data provide evidence for a universal preference for agreeable conflict cultures. This finding is encouraging, given the increase in workplace diversity and desire to be responsive to needs of equity and inclusion. Therefore, leaders and managers should strive to establish agreeable conflict norms, even in the most culturally diverse organizations. The young, college student sample may not represent all working people, although the authors only recruited students with jobs who worked 28 h per week on average.

Originality/value
This research demonstrates the value of considering multiple levels of influence on conflict, advances the theory and measurement of organizational conflict cultures and identifies powerful similarities among diverse employees.
Choice experiment to assess consumer attributes for a pharmaceutical product
Authors: Bica Vinicius Claudino , Martins Vera Lúcia Milani , Raymundo Belleza Mauricio , Lermen Fernando Henrique , Echeveste Márcia Elisa Soares 
Published: February 2023

Purpose
This study aims to identify the appropriate combination of attributes that must be present to deliver value in a new product for a pharmaceutical enterprise.

Design/methodology/approach
The application object was the Metronidazole analytical standard, using choice experiments to product attributes and price.

Findings
The practical results indicate that consumer value anticipation allows entry into the market with higher competitiveness.

Originality/value
Conceptually, it uses a value delivery approach, generally applied to large companies, in a case aimed at enterprises. In practice, for the enterprise studied, the value anticipation by the consumer allows entry into the market with higher competitiveness.
Exploring innovative strategies in problem based learning to contribute to sustainable development: a case study
Authors: Carrió Llach Mar , Llerena Bastida Maria 
Published: February 2023

Purpose
Higher education institutions (HEIs) have a great responsibility to put education for sustainable development at the centre of their work. Curricula should therefore start to incorporate the sustainable development goals (SDGs) and key competencies in sustainability, and research should be carried out to determine effective learning methods for this. This study aims to explore the usefulness of problem-based learning (PBL) approaches to train biomedical students in sustainability and to provide some recommendations for the design and implementation of new PBL-SDG scenarios.

Design/methodology/approach
Two PBL-SDG scenarios were designed, implemented and evaluated for 110 students of human biology degree. Learning outcomes and student perceptions of this approach were analysed through questionnaires, student productions, non-participant observation and focus groups.

Findings
The results show that the PBL-SDG scenarios effectively addressed several SDGs and sustainability competencies in a transversal, collaborative and innovative manner. According to student perceptions, the elements that contributed most to the development of these competencies were emotional involvement with the scenario, reflection on their own actions, freedom to approach the problem and tutors who empowered them with their proposals.

Originality/value
The PBL-SDG approach presented in this study is an example of a pedagogical strategy that can help HEIs educate their learners as key change agents. The findings of this study provide evidence for this important aspect and give guidelines and strategies to successfully designing and implementing such methodologies in biomedical education.
Members' perspectives of good governance practice of Thailand's credit union cooperatives
Authors: Kumkit Tidarat , Anh Dao Le Trang , Gan Christopher , Hu Baiding 
Published: February 2023

PurposeThis study explores the awareness (AWN) levels of good governance amongst Thai credit union cooperatives' (CUCs) members and the factors hindering good governance practice in Thai CUCs.Design/methodology/approachThis study used a survey questionnaire from 629 members of 36 selected CUCs in Thailand. This study analysed the determinants of governance AWN levels of Thai CUCs' members using the ordered probit model. The study also employs OLS estimation to investigate the factors hindering good governance practices.FindingsThe study shows that members of different CUC types and sizes have different levels of governance AWN. Members' characteristics, experiences, and perceptions significantly influence CUC members' AWN of governance issues. The findings also suggest that a lack of morality, transparency, participation, responsibility and accountability are key obstacles that hinder good governance practices of Thai CUCs.Originality/valueThis is the first study that attempts to assess the level of AWN amongst Thai CUCs' members in different CUC sizes and types. This is also the first research that identifies the factors that hinder good governance practice in Thai CUCs based on members' evaluations. The study's findings provide important reference and implications for Thai policy makers and CUCs' board of managers to enhance members' AWN and CUCs' governance performance, and thus increase income and living standard of CUCs' members in the long term.
Sustainability reporting, board gender diversity and earnings management: evidence from East Africa community
Authors: Githaiga Peter Nderitu 
Published: February 2023

PurposeThe purpose of this study is to investigate the moderating effect of board gender diversity on the relationship between sustainability reporting (SR) and earnings management (EM) in the East Africa Community (EAC).Design/methodology/approachThe study analyzed a sample of 71 publicly traded companies from 2011 to 2021.FindingsThe study finds that both SR and board gender diversity have a negative and significant effect on EM and that board gender diversity moderates the relationship between SR and EM.Practical implicationsThe findings suggest that boards should support the adoption of SR and increase female representation as a practical way to reduce EM. Policymakers should also implement appropriate measures, such as imposing mandatory SR and gender quotas on corporate boards, to address EM.Originality/valueThis research adds to the limited knowledge of SR and EM in the EAC and also fills a gap in the existing literature by investigating the influence of board gender diversity on the link between SR and EM.
Nestlé India Limited: comeback after the Maggi fiasco
Authors: Akhter P. Sohana , Prusty Sanjana , Jena Lalatendu Kesari 
Published: February 2023

Research methodology
We have used data mostly from published sources like The Economic Times, Forbes, The Times of India and the annual reports of Nestlé India Ltd. Because we classify it as a Teaching Case Study as per the guidelines of Emerald Publishing, we have ensured that any data presented in the case has been acquired only from published sources and is not internal company data. Citations have also been provided wherever necessary.

Case overview/synopsis
On 6 June 2015, Nestlé India’s top product Maggi instant noodles was banned nationwide for an unspecified period. The ban was imposed due to allegations of Maggi containing high amounts of lead and message, and consequently violating the food safety standards. What followed was the destruction of massive stocks of Maggi which had been taken off from shelves of stores countrywide. Furthermore, the company faced a huge blow financially as its sales plummeted. This case delves into how Nestlé India adopted relevant strategies to successfully avert the Maggi crisis. Some remedial measures included appointing a Managing Director who understood the market, improving the communication channel and boosting the churn out of new products along with greater emphasis on marketing and advertising.

Complexity academic level
This case is aimed mainly at undergraduate level students in the field of management studies and public relations management. This case is also relevant for students pursuing a specialization in Crisis Communication, Public Relations, Marketing and Organizational Change.