bluuebunny/crossref_metadata_2025_split huggingface dataset:
Next-generation sequencing analysis of the molecular spectrum of thalassemia in Southern Jiangxi, China
Authors: Yang Tong , Luo Xuemei , Liu Yanqiu , Lin Min , Zhao Qinfei , Zhang Wenqian , Chen Zhigang , Dong Minghua , Wang Junli , Zhang Xiaokang , Zhong Tianyu 
Published: January 2023

AbstractBACKGROUND:Thalassemia is an extremely prevalent monogenic inherited blood disorder found in southern China. It is important to comprehensively understand the molecular spectrum of thalassemia in an area of such high prevalence of thalassemia before taking appropriate actions for the prevention and treatment of this disorder. Herein, we explored the clinical feasibility of using next-generation sequencing (NGS) for large-scale population screening to illustrate the prevalence and spectrum of thalassemia in Southern Jiangxi.METHODS:Blood samples collected from 136,312 residents of reproductive age in Southern Jiangxi were characterized for thalassemia by NGS. A retrospective analysis was then conducted on blood samples determined to be positive for thalassemia.RESULTS:In total, 19,827 (14.545%) subjects were diagnosed as thalassemia carriers, and the thalassemia prevalence rate significantly varied by geographical region (p < 0.001). A total of 40 α-thalassemia genotypes including 21 rare genotypes were identified, with --SEA/αα being the most prevalent genotype. 42 β-thalassemia genotypes including 27 rare genotypes were identified, with the most common mutation IVS-II-654 (C>T) accounting for 35.257% of these β-thalassemia genotypes. Furthermore, 74 genotypes were identified among 608 individuals with combined α- and β-thalassemia. Notably, most individuals with rare thalassemia mutations had mildly abnormal hematologic parameters including microcytic hypochromia.CONCLUSIONS:Our findings demonstrate the great heterogeneity and diverse spectrum of thalassemia in Southern Jiangxi, emphasizing the importance and necessity of persistent prevention and control of thalassemia in this region. Additionally, our findings further suggest that NGS can effectively identify rare mutations and reduce the misdiagnosis rate of thalassemia.
Online Fake Logo Detection System
Authors: Tanniru Vivek , Bhattacharya Tathagata 
Published: January 2023

Abstract
With the increasing prevalence of online fraud and the use of fake logos to deceive consumers, there is a need for effective methods to detect and prevent the use of fake logos on the internet. In this paper, we propose a method for detecting fake logos using machine learning techniques. Our approach involves extracting features from the logos and training a classifier to distinguish between real and fake logos. We evaluate the performance of our method on a dataset of real and fake logos and demonstrate its effectiveness in detecting fake logos with high accuracy. Every day, hundreds of domain names, websites and logos are being cloned by cyber criminals who want to gain your trust so they can steal your data. It is becoming a big issue in the online world and needs to be addressed. This article will discuss the initial project background of our new Online Fake Logo Detection System
Classification of Stable Solutions to a Fractional Elliptic Equations with Robin boundary value
Authors: Zaidi Cherif , Ghabi Khouloud 
Published: January 2023

Abstract
In this article, we study the nonexistence of solution with finite Morse index for the following nonlinear elliptic equation for fractional Laplacian : (−∆) s u + λu = |u| p−1 u in R n + with ∂u/∂ν + cu = 0 on ∂R n +. where n ≥ 2s, 0 < s < 1, λ ≥ 0, c ≥ 0 and p > 1. The main methods used are the integral estimates, the Pohozaev-type identity and the monotonicity formula.
PACS: Primary : 35J55, 35J65 ; Secondary : 35B65.
Probing the plant transcriptomes for novel waikaviral sequences broadens the host range and genetic diversity of waikaviruses
Authors: Sidharthan V Kavi , Rajeswari V , Baranwal V K 
Published: January 2023

AbstractWaikaviruses (family:Secoviridae) are monopartite, positive sense, single-stranded RNA viruses that cause economically important diseases in plants. Despite their importance, waikaviruses are poorly understood and only ten members are currently recognized. In the present study on Sequence Read Archive (SRA)-based data-driven virus discovery (DDVD), we identified twenty-two putative novel waikaviruses, nearly doubling the number of known waikaviruses, in SRA libraries of diverse plant species, starting from ferns to trees. Besides, a highly divergent secoviral sequence with distinct genome features, yet related to waikaviruses, was identified in a wheat transcriptome. Other significant findings of the study include identification of a novel waikavirus in a library derived from diseased water chestnut sample wherein a novel caulimovirus was reported, prediction of coiled-coils in the hypothetical protein region of waikaviral polyprotein alignment and phylogenetic clustering of tree-infecting waikaviruses in a single sub-group. The findings of the study not only reiterates the importance of DDVD in unveiling the hitherto hidden viral sequences in plant SRA libraries but also deepens our understanding of waikaviral diversity.
Micropropagation of plants of the genus Actinidia Lindl.
Authors: Kyienko Z. B. , Kimeichuk I. V. , Matskevych V. V. 
Published: January 2023

Purpose. Analysis of plant micropropagation technologies for the creation of viable interspecific hybrids and varieties of Actinidia Lindl.
Methods. General scientific – hypothesis, experiment, observation, analysis, synthesis method for drawing conclusions.
Results. The introduction of in vitro technologies is now becoming the dominant commercial method of large-scale and rapid production of seedlings with stable inheritance of variety traits, high multiplication rate, preservation of economically valuable traits in the absence of production seasonality and time constraints. In addition to reproduction, the breeding process is also accelerated, including mutagenesis and hybridization. It is important to obtain not only a sterile explant, but also a morphogenically active one, that is, a plant that takes roots and subsequently regenerates in vitro. The best in terms of decontamination efficiency is the method of treatment with hypochlorite and the addition of PPM biocide to the nutrient medium, but under these conditions, the lowest survival of explants in all samples was noted. The efficiency of introduction into aseptic culture at the first stage of micropropagation is also affected by the biological characteristics of the primary explants. In studies with nutrient media for A. arguta, it was found that of the elements of mineral nutrition, only 11 ions are necessary for life: five macro- (N, K, P, Mg, S) and six microelements (Cl, Fe, B, Mo, Na, I). Plants in vitro have a lower dry matter content and a greater amount of moisture, including free moisture, which is quickly lost when the water balance is disturbed.
Conclusions. The abi­lity to regenerate is more pronounced in the species A. chinensis and A. deliciosa, and to a lesser extent in A. arguta. For A. chinensis, the use of hydroponic technology for the adaptation of regenerants at the ex vitro stage is effective.
A charged nanocomposite membrane via co-deposition of gallic acid and polyethyleneimine-silver for improving separation and antibacterial properties
Authors: Zhu Yihang , Huang Zhonghua , Tang Mengdi , Li Qunxia , Liu Yulong , Bai Xinhui 
Published: January 2023

Abstract
Pharmaceuticals have been continuously detected from surface water and groundwater. In order to improve the rejection performance of pharmaceuticals by a nanofiltration membrane (NF), a positively charged membrane was prepared by co-deposition of natural gallic acid and polyethyleneimine on the polyacrylonitrile hydrolysis membrane. Effects of gallic acid concentration, polyethylene imine concentration, reaction time, and the molecular weight of polyethylene imine were documented. The physical and chemical properties of the membrane were also investigated by surface morphology, hydrophilicity, surface charge, and molecular weight cut-off. The optimized membrane had a molecular weight cut-off of about 958 Da and possessed a pure water permeability of 74.21 L·m−2·h−1·MPa−1. The results exhibited salt rejection in the following order: MgCl2 &gt; CaCl2 &gt; MgSO4 &gt; Na2CO3 &gt; NaCl &gt; Na2SO4, while the rejection ability of pharmaceuticals is as follows: amlodipine &gt; atenolol &gt; carbamazepine &gt; ibuprofen, suggesting that the positively charged membrane has enhanced retention to both divalent cations and charged pharmaceuticals. In addition, the antibacterial membrane was obtained by loading silver nanoparticles onto the positively charged membrane, which greatly improved the antibacterial ability of the membrane.
Targeting Ferroptosis as a New Approach for Radiation Protection and
Mitigation
Authors: Shaghaghi Zahra , Salari Arsalan , Jalali Fatemeh , Alvandi Maryam , Farzipour Soghra , Zarei Polgardani Nasim 
Published: January 2023

Abstract:
Radiation-induced normal cell toxicity (RINCT) is a major factor to consider while
treating any ailment with radiotherapy. Clinical irradiation of tumors necessitates an understanding
of the potential efficacy of radiation protective agents in reducing radiation damage to healthy
tissues and their effects on tumor tissue radiosensitivity. Ferroptosis is a relatively new form of
iron-dependent cell death that has been linked to a variety of disease pathologies. The key mediators
of ferroptosis have been identified as lipid peroxidation and iron metabolism. Lipid peroxidation
is the result of a reaction between reactive oxygen (ROS) and reactive nitrogen species (RNS)
with phosphatidylethanolamine-containing polyunsaturated fatty acids (PUFAs). Ferroptosis inhibitors
have been demonstrated to have anti-inflammatory effects in animal models of disease. It
was recently shown that ionizing radiation (IR) generates severe ferroptosis, a critical component
of RT-mediated normal cell toxicity. These findings support the use of ferroptosis inhibitor treatments
for the treatment of radiation normal cell toxicity. Targeting lipid metabolic substrates and
controlling ferroptosis by radiation could reduce toxicity and improve clinical outcomes. In this
study, we address the relationships between radiotherapy and various types of radiation-induced
cell death, and we discuss the interactions between ferroptosis and other kinds of controlled cell
death generated by radiotherapy, and we investigate combination treatment options targeting ferroptosis
in radiotherapy. This review will be a foundation for future research on ferroptosis in radiotherapy.
Additionally, the relevant patents on ferroptosis inhibitors with various therapeutic
potentials have been discussed.
Resignificación de las prácticas pedagógicas: la reflexión docente y su implicación con la primera infancia
Authors: Impatá Álvarez Diana Marcela 
Published: January 2023

El trabajo de investigación asume el análisis, resignificación y reflexión generada desde la práctica pedagógica docente al proceso de enseñanza y aprendizaje de niños y niñas de primera infancia según las políticas de educación inicial, busca determinar las habilidades pedagógicas que le dan sentido a la práctica y el impacto en la población objeto, respondiendo al cuarto objetivo de desarrollo sostenible, educación de calidad.
Parte de un enfoque cualitativo e interpretativo, basado en el modelo socio-constructivista permeado por los espacios con sentido pedagógico estableciendo categorías de interpretación y resultados de datos a través del diseño y ejecución de un proyecto de aula permitiendo pensar las actuaciones docentes desde un proceso cíclico de reflexión-acción-reflexión.
De acuerdo con los resultados obtenidos, se establece que el docente hace una reflexión profunda y pertinente de su quehacer profesional, describirlo, explicarlo y argumentarlo de manera efectiva y analítica para la resignificación de la educación inicial.
VEGF-A: A Novel Mechanistic Link Between CYP2C-Derived EETs and Nox4 in Diabetic Kidney Disease
Authors: Njeim Rachel , Braych Kawthar , Ghadieh Hilda E. , Azar Nadim S. , Azar William S. , Dia Batoul , Leone Angelo , Cappello Francesco , Kfoury Hala , Harb Frederic , Jurjus Abdo R. , Eid Assaad A. , Ziyadeh Fuad N. 
Published: January 2023

Diabetes is associated with decreased epoxyeicosatrienoic acid (EET) bioavailability and increased levels of glomerular vascular endothelial growth factor A (VEGF-A) expression. We examined whether a soluble epoxide hydrolase inhibitor protects against pathologic changes in diabetic kidney disease and whether the inhibition of the VEGF-A signaling pathway attenuates diabetes-induced glomerular injury. We also aimed to delineate the cross talk between cytochrome P450 2C (CYP2C)–derived EETs and VEGF-A. Streptozotocin-induced type 1 diabetic (T1D) rats were treated with 25 mg/L of 12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA) in drinking water for 6 weeks. In parallel experiments, T1D rats were treated with either SU5416 or humanized monoclonal anti–VEGF-A neutralizing antibody for 8 weeks. Following treatment, the rats were euthanized, and kidney cortices were isolated for further analysis. Treatment with AUDA attenuated the diabetes-induced decline in kidney function. Furthermore, treatment with AUDA decreased diabetes-associated oxidative stress and NADPH oxidase activity. Interestingly, the downregulation of CYP2C11-derived EET formation is found to be correlated with the activation of the VEGF-A signaling pathway. In fact, inhibiting VEGF-A using anti-VEGF or SU5416 markedly attenuated diabetes-induced glomerular injury through the inhibition of Nox4-induced reactive oxygen species production. These findings were replicated in vitro in rat and human podocytes cultured in a diabetic milieu. Taken together, our results indicate that hyperglycemia-induced glomerular injury is mediated by the downregulation of CYP2C11-derived EET formation, followed by the activation of VEGF-A signaling and upregulation of Nox4. To our knowledge, this is the first study to highlight VEGF-A as a mechanistic link between CYP2C11-derived EET production and Nox4.

Article Highlights
Diabetes is associated with an alteration in cytochrome P450 2C11 (CYP2C11)–derived epoxyeicosatrienoic acid (EET) bioavailability. Decreased CYP2C11-derived EET bioavailability mediates hyperglycemia-induced glomerular injury. Decreased CYP2C11-derived EET bioavailability is associated with increased reactive oxygen species production, NADPH oxidase activity, and Nox4 expression in type 1 diabetes. Decreased CYP2C11-derived EET formation mediates hyperglycemia-induced glomerular injury through the activation of the vascular endothelial growth factor A (VEGF-A) signaling pathway. Inhibiting VEGF signaling using anti-VEGF or SU5416 attenuates type 1 diabetes–induced glomerular injury by decreasing NADPH oxidase activity and NOX4 expression.
The Effect of Beneficial Autochthonous Microorganisms (Bams) on the Chemical Quality of Agricultural Soils and Olive Production in the Wilaya of Tlemcen (Western of Algeria)
Authors: Dahmani Rachid , Borsali Amine Habib , Merzouk Abdessamad , Zouidi Mohamed , Aouadj Sid Ahmed , Khatir Hadj 
Published: January 2023

Abstract
Beneficial Autochthonous Microorganisms (BAMs) are a multi-purpose technology that has already been used in Latin America and Southeast Asia but is still unknown in Africa and particularly Algeria. The aim is to examine the effect of BAMs on agricultural soils and olive fields. The study was carried out in two degraded olive fields located in the Oued Tafna basin west of Tlemcen (The Semi-arid region in Western Algeria). The objective is to evaluate the effectiveness of BAMs on the chemical characteristics of the soils, particularly in terms of organic matter (MO), pH, electrical conductivity (EC), total limestone (CaCO3), moisture, organic carbon (C) and olive production and its weight. Statistical analysis (Independent Test) between all groups (treated and controls) showed a significant difference, in conductivity (EC) where we recorded “0.214±0.03μs /cm “ in treated soils vs. “0.198±0.029μs/cm “ in control soils a different statistical significance was recorded for the other parameters, however, it was observed that there is a relative increase in these parameters in the soils of the treated groups such as carbon (C): “2.85±1.06%” organic matter (MO): “4.91± 1.83%”, “pH=7.81±0.225”, “CaCO3 27.76±4.99% and a decrease in humidity “15.11±3.77%” compared to control soils. The results of statistical analyses (Mann-Whitney Test and Suite Test) of olive production showed a clear alternation (every other year) in fruit production in the control group and a positive effect of BAMs on olive production in the treated group, with a slight increase of production (19 kg in 2019 vs. 30 kg in 2020), but without any significant difference regarding the weight of the fruit between the two sessions. However, a highly significant difference “p=0.00<0.001” in fruit weight was noted between the treated groups “3.908g” and “5.70g” and the control ones “4.40g “ and “5.00g” in 2019 and 2020 respectively. The use of MAB is an interesting technique to restore the chemical properties of degraded soils, and increase olive tree production. To achieve more reproducible results (in terms of quantity and quality), sufficient doses of these BAMs and periodicals must be added to provide a good nutritional supplement and reduce farmers’ use of pesticides and mineral fertilizers.
On unique and non-unique fixed point in parametric N

b

−metric spaces with application
Authors: Petwal Sudheer , Tomar Anita , Joshi Meena 
Published: January 2023

Abstract
We propose 𝒮𝒜, η−𝒮𝒜, η−𝒮 𝒜min, and 𝒮𝒜η,δ,ζ−contractions and notions of η−admissibility type b and η
b
−regularity in parametric N

b
-metric spaces to determine a unique fixed point, a unique fixed circle, and a greatest fixed disc. Further, we investigate the geometry of non-unique fixed points of a self mapping and demonstrate by illustrative examples that a circle or a disc in parametric N

b
−metric space is not necessarily the same as a circle or a disc in a Euclidean space. Obtained outcomes are extensions, unifications, improvements, and generalizations of some of the well-known previous results. We provide non-trivial illustrations to exhibit the importance of our explorations. Towards the end, we resolve the system of linear equations to demonstrate the significance of our contractions in parametric N

b
−metric space.
Some results on Caristi type coupled fixed point theorems
Authors: Şahin İlker , Telci Mustafa 
Published: January 2023

Abstract
In this work we define the concepts of the coupled orbit and coupled orbitally completeness. After then, using the method of Bollenbacher and Hicks [8], we prove some Caristi type coupled fixed point theorems in coupled orbitally complete metric spaces for a function P : E × E → E. We also give two examples that support our results.
On Chern classes of the tensor product of vector bundles
Authors: Szilágyi Zsolt 
Published: January 2023

AbstractWe present two formulas for Chern classes (polynomial) of the tensor product of two vector bundles. In the first formula the Chern polynomial of the product is expressed as determinant of a polynomial in a matrix variable involving the Chern classes of the first bundle with Chern classes of the second bundle as coefficients. In the second formula the total Chern class of the tensor product is expressed as resultant of two explicit polynomials. Finally, formulas for the total Chern class of the second symmetric and the second alternating products are deduced.
Employee scheduling and maintenance planning for safety systems at the remotely located oil and gas industrial facilities
Authors: Redutskiy Yury , Balycheva Marina , Dybdahl Hendrik 
Published: January 2023

Abstract
The safety of operations is vital in any process in the oil and gas sector, especially given that increasingly more hydrocarbon reserves are discovered in non-conventional remote and Arctic locations. Safety systems are designed as a part of a complex IT system for process control. The design of these systems is conducted in the form of an engineering project. This research presents a decision-making framework to facilitate formulating clear and comprehensive recommendations for the requirements specification developed for the safety systems. The contribution of this research to the strategic planning area of IT solutions for hazardous industrial facilities is integrating the problems of designing a safety system, planning its maintenance, and scheduling the employees to conduct the required maintenance. With this joint decision-making, it is possible to explore trade-offs between investments into the systems’ complexity and workforce-related expenditures throughout the solution’s lifecycle. The reliability modelling is conducted with the help of Markov analysis. The multi-objective decision-making framework is employed to deduce straightforward requirements to the safety system design, maintenance strategy, and workforce organisation. This research is relevant to managing the petroleum sector engineering projects with regard to the design of technological solutions.
Study on the improvement of medical service quality in Beijing’s Tianqiao Community Health Service Centre
Authors: He Qiong , Li Qixiao , Chen Jindong 
Published: January 2023

Abstract
Within the context of the COVID-19 pandemic, community-level medical institutions as health service centres have been gaining importance in the medical reform expansion. As prior research has not fully addressed how to index and evaluate the quality of medical service, this article proposes a framework based on the service quality gap theory and the three-faceted “structure–process–outcome” quality evaluation theory. The study took the medical services at Beijing’s Tianqiao Community Health Service Centre as an example to construct an index system for medical service quality evaluations. Data was collected from 211 people, and SPSS software was used for data processing and analysis. Due to the COVID-19 pandemic, patients without serious diseases tend to choose community hospitals to reduce their infection risk. As a result, they have growing requirements for clinics to have more departments and specialists. The studied community health service centre has encountered difficulties connected to low patient expectations, a poor medical environment, outdated hardware and equipment, and a low level of medical services. Some suggestions have been made to add specialised departments and consider the convenience of medical treatment for the elderly.