bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimal Frequency of Hepatitis C Virus (HCV) RNA Testing for Detection of Acute HCV Infection Among At-risk People With Human Immunodeficiency Virus: A Multicenter Study
Authors: Sun Hsin-Yun , Liou Bo-Huang , Chen Tun-Chieh , Yang Chia-Jui , Huang Sung-Hsi , Lu Po-Liang , Huang Chung-Hao , Tsai Mao-Song , Cheng Shu-Hsing , Lee Nan-Yao , Ko Wen-Chien , Chen Yen-Hsu , Liu Wang-Da , Lin Shang-Yi , Lin Shih-Ping , Chen Po-Lin , Syue Ling-Shan , Huang Yu-Shan , Chuang Yu-Chung , Chen Cheng-Bin , Chang Ya-Ting , Lee Yuan-Ti , Hsieh Szu-Min , Su Li-Hsin , Cheng Chien-Yu , Hung Chien-Ching 
Published: June 2023

Abstract
Using 3-stage pooled-plasma hepatitis C virus (HCV) RNA testing performed quarterly among at-risk people with human immunodeficiency virus (PWH), we found that if testing had been performed every 6 or 12 months, 58.6%–91.7% of PWH who recently acquired HCV would be delayed for diagnosis and might contribute to onward HCV transmission with longer durations.
A Survey of Practice Patterns for Clinical Nodal Staging Prior to Neoadjuvant Chemotherapy in Breast Cancer
Authors: Lee Han-Byoel , Lee Kyung-Hun , Song Seok Hyun , Kim Kyubo , Kim Hong Kyu , Moon Hyeong-Gon , Han Wonshik , Lee Dae-Won , Im Seock-Ah , Jang Bum-Sup , Kim Yong Bae , Yu Jonghan , Kim Jee Hyun , Park Yeon Hee , Shin Kyung Hwan , Chang Ji Hyun 
Published: June 2023

Abstract

Background
The importance of clinical staging in breast cancer has increased owing to the wide use of neoadjuvant systemic therapy (NST). This study aimed to investigate the current practice patterns regarding clinical nodal staging in breast cancer in real-world settings.

Materials and Methods
A web-based survey was administered to board-certified oncologists in Korea, including breast surgical, medical, and radiation oncologists, from January to April 2022. The survey included 19 general questions and 4 case-based questions.

Results
In total, 122 oncologists (45 radiation, 44 surgical, and 33 medical oncologists) completed the survey. Among them, 108 (88%) responded that clinical staging before NST was primarily performed by breast surgeons. All the respondents referred to imaging studies during nodal staging. Overall, 64 (52.5%) responders determined the stage strictly based on the radiology reports, whereas 58 (47.5%) made their own decision while noting radiology reports. Of those who made their own decisions, 88% referred to the number or size of the suspicious node. Of the 75 respondents involved in prescribing regimens for neoadjuvant chemotherapy, 58 (77.3%) responded that the reimbursement regulations in the selection of NST regimens affected nodal staging in clinical practice. In the case-based questions, high variability was observed among the clinicians in the same cases.

Conclusions
Diverse assessments by specialists owing to the lack of a clear, harmonized staging system for the clinical nodal staging of breast cancer can lead to diverse practice patterns. Thus, practical, harmonized, and objective methods for clinical nodal staging and for the outcomes of post-NST response are warranted for appropriate treatment decisions and accurate outcome evaluation.
La pharmacothérapie en fonction des gènes
Authors: Rassekh S Rod , Rieder Michael , ‘t Jong Geert 
Published: June 2023

Résumé
Depuis vingt ans, le savoir médical sur le rôle des facteurs génétiques de variabilité a énormément évolué, tant à l’égard des maladies humaines que de la réponse aux médicaments. Ce savoir se traduit de plus en plus par des directives qui influent sur la posologie, la surveillance de l’efficacité et de l’innocuité et la détermination de la pertinence d’agents particuliers pour traiter les patients. Santé Canada et la Food and Drug Administration des États-Unis recommandent d’utiliser l’information génétique pour orienter la posologie de plus de 20 médicaments. Il n’existe actuellement pas de directives pédiatriques complètes pour aider les professionnels de la santé à utiliser la génétique afin d’établir la posologie, l’innocuité et l’efficacité des médicaments chez les enfants, et ces directives s’imposent d’urgence. Le présent document de principes aide le clinicien à comprendre le rôle de la pharmacogénétique et à utiliser l’information qu’il en tire pour prescrire des médicaments en pédiatrie.
Histone Deacetylases Regulate MORE AXILLARY BRANCHED 2-Dependent Germination of Arabidopsis thaliana
Authors: Temmerman Arne , De Keyser Annick , Boyer François-Didier , Struk Sylwia , Goormachtig Sofie 
Published: June 2023

Abstract
Under specific conditions, the germination of Arabidopsis thaliana is dependent on the activation of the KARRIKIN INSENSITIVE 2 (KAI2) signaling pathway by the KAI2-dependent perception of karrikin or the artificial strigolactone analogue, rac-GR24. To regulate the induction of germination, the KAI2 signaling pathway relies on MORE AXILLARY BRANCHED 2- (MAX2-)dependent ubiquitination and proteasomal degradation of the repressor protein SUPPRESSOR OF MAX2 1 (SMAX1). It is not yet known how the degradation of SMAX1 proteins eventually results in the regulation of seed germination, but it has been hypothesized that SMAX1-LIKE generally functions as transcriptional repressors through the recruitment of co-repressors TOPLESS (TPL) and TPL-related, which in turn interact with histone deacetylases. In this article, we show the involvement of histone deacetylases HDA6, HDA9, HDA19 and HDT1 in MAX2-dependent germination of Arabidopsis, and more specifically, that HDA6 is required for the induction of DWARF14-LIKE2 expression in response to rac-GR24 treatment.
Asymmetric variation in DNA methylation during domestication and de-domestication of rice
Authors: Cao Shuai , Chen Kai , Lu Kening , Chen Shiting , Zhang Xiyu , Shen Congcong , Zhu Shuangbin , Niu Yanan , Fan Longjiang , Chen Z Jeffrey , Xu Jianlong , Song Qingxin 
Published: June 2023

AbstractHundreds of plant species have been domesticated to feed human civilization, while some crops have undergone de-domestication into agricultural weeds, threatening global food security. To understand the genetic and epigenetic basis of crop domestication and de-domestication, we generated DNA methylomes from 95 accessions of wild rice (Oryza rufipogon L.), cultivated rice (Oryza sativa L.) and weedy rice (O. sativa f. spontanea). We detected a significant decrease in DNA methylation over the course of rice domestication but observed an unexpected increase in DNA methylation through de-domestication. Notably, DNA methylation changes occurred in distinct genomic regions for these 2 opposite stages. Variation in DNA methylation altered the expression of nearby and distal genes through affecting chromatin accessibility, histone modifications, transcription factor binding, and the formation of chromatin loops, which may contribute to morphological changes during domestication and de-domestication of rice. These insights into population epigenomics underlying rice domestication and de-domestication provide resources and tools for epigenetic breeding and sustainable agriculture.
The African cancer burden: what is the potential role of modern oncology innovation in reducing the continent’s rapidly rising mortality?
Authors: Awuah Wireko Andrew , Ng Jyi Cheng , Mehta Aashna , Nansubuga Esther Patience , Abdul-Rahman Toufik , Kundu Mrinmoy , Ou Yong Brian M , Kalmanovich Jacob , Hussain Aysha , Jessica Otumara Urowoli , Kamanousa Karl , Akilimali Aymar , Sikora Kateryna , Isik Arda 
Published: June 2023

Abstract
With increasing prevalence and an expected rise in disease burden, cancer is a cause of concern for African healthcare. The cancer burden in Africa is expected to rise to 2.1 million new cases per year and 1.4 million deaths annually by the year 2040. Even though efforts are being made to improve the standard of oncology service delivery in Africa, the current state of cancer care is not yet on par with the rise in the cancer burden. Cutting-edge technologies and innovations are being developed across the globe to augment the battle against cancer; however, many of them are beyond the reach of African countries. Modern oncology innovations targeted to ward Africa would be promising to address the high cancer mortality rates. The innovations should be cost-effective and widely accessible to tackle the rapidly rising mortality rate on the African continent. Though it may seem promising, a multidisciplinary approach is required to overcome the challenges associated with the development and implementation of modern oncology innovations in Africa.
Biallelic variants in RBM42 cause a multisystem disorder with neurological, facial, cardiac, and musculoskeletal involvement
Authors: Chen Yiyao , Yang Bingxin , Zhang Xiaoyu Merlin , Chen Songchang , Wang Minhui , Hu Liya , Pan Nina , Li Shuyuan , Shi Weihui , Yang Zhenhua , Wang Li , Tan Yajing , Wang Jian , Wang Yanlin , Xing Qinghe , Ma Zhonghua , Li Jinsong , Huang He-Feng , Zhang Jinglan , Xu Chenming 
Published: June 2023

Abstract
Here, we report a previously unrecognized syndromic neurodevelopmental disorder associated with biallelic loss-of-function variants in the RBM42 gene. The patient is a 2-year-old female with severe central nervous system (CNS) abnormalities, hypotonia, hearing loss, congenital heart defects, and dysmorphic facial features. Familial whole-exome sequencing (WES) reveals that the patient has two compound heterozygous variants, c.304C&gt;T (p.R102*) and c.1312G&gt;A (p.A438T), in the RBM42 gene which encodes an integral component of splicing complex in the RNA-binding motif protein family. The p.A438T variant is in the RRM domain which impairs RBM42 protein stability in vivo. Additionally, p.A438T disrupts the interaction of RBM42 with hnRNP K, which is the causative gene for Au-Kline syndrome with overlapping disease characteristics seen in the index patient. The human R102* or A438T mutant protein failed to fully rescue the growth defects of RBM42 ortholog knockout ΔFgRbp1 in Fusarium while it was rescued by the wild-type (WT) human RBM42. A mouse model carrying Rbm42 compound heterozygous variants, c.280C&gt;T (p.Q94*) and c.1306_1308delinsACA (p.A436T), demonstrated gross fetal developmental defects and most of the double mutant animals died by E13.5. RNA-seq data confirmed that Rbm42 was involved in neurological and myocardial functions with an essential role in alternative splicing (AS). Overall, we present clinical, genetic, and functional data to demonstrate that defects in RBM42 constitute the underlying etiology of a new neurodevelopmental disease which links the dysregulation of global AS to abnormal embryonic development.
Yeast biopanning against site-specific phosphorylations in tau
Authors: Arbaciauskaite Monika , Pirhanov Azady , Ammermann Erik , Lei Yu , Cho Yong Ku 
Published: June 2023

Abstract
The detection of site-specific phosphorylation in the microtubule-associated protein tau is emerging as a means to diagnose and monitor the progression of Alzheimer’s Disease and other neurodegenerative diseases. However, there is a lack of phospho-specific monoclonal antibodies and limited validation of their binding specificity. Here, we report a novel approach using yeast biopanning against synthetic peptides containing site-specific phosphorylations. Using yeast cells displaying a previously validated phospho-tau (p-tau) single-chain variable region fragment (scFv), we show selective yeast cell binding based on single amino acid phosphorylation on the antigen. We identify conditions that allow phospho-specific biopanning using scFvs with a wide range of affinities (KD = 0.2 to 60 nM). Finally, we demonstrate the capability of screening large libraries by performing biopanning in 6-well plates. These results show that biopanning can effectively select yeast cells based on phospho-site specific antibody binding, opening doors for the facile identification of high-quality monoclonal antibodies.
Why States Preempt City Ordinances: The Case of Workers’ Rights Laws
Authors: Goodman Christopher B , Hatch Megan E 
Published: June 2023

Abstract
Despite being popular with the public and preventing racial and economic inequality, states often preempt their local governments’ ability to adopt workers’ rights laws. We test several competing theories of preemption (ideology, political institutions, interest group involvement, demographics, and policy diffusion) using a time-series, cross-sectional approach. Using data on state legislative activity from 1993 to 2018, we find that increasing legislative conservatism, and more unified political control of the state government, regardless of party, are associated with a higher risk of preempting local workers’ rights laws, all else equal. Our focus on legislative ideology, a more precise measure than party control at the subnational level, as the nexus of preemption activity helps clarify prior contradictory results in the literature. For those looking to prevent or overturn workers’ rights preemptions, the most direct approach appears to be to change the ideology of state legislatures.
Protection of SARS-CoV-2 trial vaccines in human is a function of the viral genomes
Authors: Alhaji Saganuwan Saganuwan 
Published: June 2023

Abstract

Objectives
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has devastated mankind with complaints that many vaccines developed against the disease could not offer protection and guarantee safety, hence many vaccinated individuals either came down with the infection and died or transmitted the infection. Therefore permanent immunization may become difficult due to interplay between the viral and human genomes.

Methods
In view of this, literatures were searched on the trial vaccines against SARS-CoV-2. Concentration log10 (copies/ml) of the virus, log10 viral copies, populations of human vaccinated, protection indices of the vaccines, population of Tcells, lethal concentration 1 of the virus, doses of the trial vaccines and vaccine regimens of the SARS-CoV-2 obtained from membrane ribonucleic acid, replication-defective viral vector, inactivated pathogen vaccine, protein subunit vaccine, virus-like particle and deoxyribonucleic acid vaccine (DNAV) were used with an intent to assessing the pathogenicity and virulence of the vaccines.

Key findings
Findings have shown that virion of 3.3 × 108–9 could kill humans over a period of ≥20 days, and 109–11 virions have killed 3 in every 100 humans. However, viral load of detection (3.22 × 103), positivity threshold (3.3 × 103) and index patient value (6.6 × 106) respectively have been established. The protection index is between 11% and 99%.

Conclusions
The viral load of coronavirus found in the affected patients was relatively high and could be fatal. However, DNAV-based vaccine (2 mg) administered twice 4 weeks apart provided the best protection index that lasted for 40–60 days.
Borders and Boundaries in Daily Urban Mobility Practices of Refugees in Cape Town, South Africa
Authors: Chekero Tamuka 
Published: June 2023

Abstract
This ethnographic study investigates the ways in which the label of refugee creates and reinforces particular kinds of boundaries and borders in mobility and survival efforts. Specifically, it looks at refugees’ movement in Bellville, Cape Town, to see how state and local borders affect intra-city refugee movement and how refugees deal with these obstacles in order to find localised protection and long-term survival strategies. This article builds on Nyamnjoh’s work on “nimble-footedness” and “conviviality” to show how refugees use nimbleness to form convivial social networks, which they convert into social capital. Despite their accommodation in the National Constitution, this article shows how refugees in Bellville are continually excluded and marginalised due to state and local level boundaries and borders. This article examines police blockages and state-based COVID-19 lockdowns and curfews and their effects on refugees and their movement. The findings show that, while having significant impact on their daily lives, refugees still find ways to negotiate through, around or avoid these barriers, as they navigate the city in search of livelihoods, services, and forms of protection. By utilising nimble-footedness and conviviality, this article depicts refugees as rational individuals who rely on numerous interconnections, inseparable entanglements, and creative interdependencies to survive.
Science diplomacy from a nation-state’s perspective: a general framing and its application to Global South countries
Authors: Ruffini Pierre-Bruno , Krasnyak Olga 
Published: June 2023

Abstract
Based on International Relations (IRs) grounding theories, this article outlines a realist-constructivist perspective in science diplomacy when assessing a nation-state’s foreign policy decision-making and behaviour. The proposed theoretical framing helps us evaluate existing practices of science diplomacy within the larger context of IRs and allows us to better understand the increasing role of science diplomacy and its potency in the foreign policy of emerging and developing countries. The proposed exploratory research methodology outlines the contours of a science diplomacy reading grid breaking it down into the categories of objectives, strategic drivers, and tools. The data collection and semi-structured interviews with high-ranking practitioners and experts allowed us to assess the meaning of science diplomacy as understood and implemented by Global South countries and to distinguish science diplomacy practices as oriented towards the satisfaction of domestic needs and international positioning.
Scientific collaborations between Latin America and Europe: an approach from science diplomacy towards international engagement
Authors: Echeverría-King Luisa F , Fossati Aura , Raja Nussaïbah B , Bonilla Kleinsy , Urbani Bernardo , Whiffen Radenka Krsmanović , Vizinová Tereza 
Published: June 2023

Abstract
International collaborations show asymmetries and imbalances that influence how countries take part in international relations. In this context, science diplomacy (SD) could offer possibilities towards building partnerships and meaningful engagement between Europe and Latin America. The purpose of this article is to analyse how scientific collaborations between Latin American and European researchers are carried out, observing trends, behaviours, and perceptions. Qualitative methodologies and the analysis of empirical data collected through a survey allow the extraction of relevant experiences from real cases of international joint projects. Findings indicate that addressing the asymmetries in the collaboration between partnering researchers from Europe and Latin America is essential, and SD approaches may facilitate such endeavour. SD may not represent a panacea; however, it seems to facilitate the internationalisation of research in terms of mobility, international scientific collaborations, and knowledge exchange with under-represented actors in traditional international scientific schemes, such as indigenous communities.
Does providing feedback and guidance on sleep perceptions using sleep wearables improve insomnia? Findings from “Novel Insomnia Treatment Experiment”: a randomized controlled trial
Authors: Spina Marie-Antoinette , Andrillon Thomas , Quin Nina , Wiley Joshua F , Rajaratnam Shantha M W , Bei Bei 
Published: June 2023

Abstract

Study Objectives
Insomnia is a disorder diagnosed based on self-reported sleep complaints. Differences between self-reported and sensor-based sleep parameters (sleep–wake state discrepancy) are common but not well-understood in individuals with insomnia. This two-arm, parallel-group, single-blind, superiority randomized-controlled trial examined whether monitoring sleep using wearable devices and providing support for interpretation of sensor-based sleep data improved insomnia symptoms or impacted sleep–wake state discrepancy.

Methods
A total of 113 (age M = 47.53; SD = 14.37, 64.9% female) individuals with significant insomnia symptoms (Insomnia Severity Index(ISI) ≥10) from the community were randomized 1:1 (permuted block randomization) to receive 5 weeks (1) Intervention (n = 57): feedback about sensor-based sleep (Fitbit and EEG headband) with guidance for data interpretation and ongoing monitoring, and (2) Control (n = 56): sleep education and hygiene. Both groups received one individual session and two check-in calls. The ISI (primary outcome), sleep disturbance (SDis), sleep-related impairment (SRI), depression, and anxiety were assessed at baseline and post-intervention.

Results
In total, 103 (91.2%) participants completed the study. Intention-to-treat multiple regression with multiple imputations showed that after controlling for baseline values, compared to the Control group (n = 51), the Intervention group (n = 52) had lower ISI (p = .011, d = 0.51) and SDis (p = .036, d = 0.42) post-intervention, but differences in SRI, depression, anxiety, and sleep–wake state discrepancy parameters (total sleep time, sleep onset latency, and wake after sleep onset) were not meaningful (P-values &gt;.40).

Conclusions
Providing feedback and guidance about sensor-based sleep parameters reduced insomnia severity and sleep disturbance but did not alter sleep–wake state discrepancy in individuals with insomnia more than sleep hygiene and education. The role of sleep wearable devices among individuals with insomnia requires further research.

Clinical Trial Registration
The Novel Insomnia Treatment Experiment (NITE): the effectiveness of incorporating appropriate guidance for sleep wearables in users with insomnia. https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=378452, Australia New Zealand Clinical Trials Registry: ACTRN12619001636145.
Fisetin Attenuates Cellular Senescence Accumulation During Culture Expansion of Human Adipose-Derived Stem Cells
Authors: Mullen Michael , Nelson Anna Laura , Goff Alexander , Billings Jake , Kloser Heidi , Huard Charles , Mitchell John , Hambright William Sealy , Ravuri Sudheer , Huard Johnny 
Published: June 2023

Abstract
Mesenchymal stem cells (MSCs) have long been viewed as a promising therapeutic for musculoskeletal repair. However, regulatory concerns including tumorgenicity, inconsistencies in preparation techniques, donor-to-donor variability, and the accumulation of senescence during culture expansion have hindered the clinical application of MSCs. Senescence is a driving mechanism for MSC dysfunction with advancing age. Often characterized by increased reactive oxygen species, senescence-associated heterochromatin foci, inflammatory cytokine secretion, and reduced proliferative capacity, senescence directly inhibits MSCs efficacy as a therapeutic for musculoskeletal regeneration. Furthermore, autologous delivery of senescent MSCs can further induce disease and aging progression through the secretion of the senescence-associated secretory phenotype (SASP) and mitigate the regenerative potential of MSCs. To alleviate these issues, the use of senolytic agents to selectively clear senescent cell populations has become popular. However, their benefits to attenuating senescence accumulation in human MSCs during the culture expansion process have not yet been elucidated. To address this, we analyzed markers of senescence during the expansion of human primary adipose-derived stem cells (ADSCs), a population of fat-resident MSCs commonly used in regenerative medicine applications. Next, we used the senolytic agent fisetin to determine if we can reduce these markers of senescence within our culture-expanded ADSC populations. Our results indicate that ADSCs acquire common markers of cellular senescence including increased reactive oxygen species, senescence-associated β-galactosidase, and senescence-associated heterochromatin foci. Furthermore, we found that the senolytic agent fisetin works in a dose-dependent manner and selectively attenuates these markers of senescence while maintaining the differentiation potential of the expanded ADSCs.