bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_circ_0000026 Sponges miR-23a-5p and Upregulates PTEN Expression to Suppress Gastric Cancer Cell Progression
Authors: Meng Qiuping , Xue Fenggui , Luo Zhifei , Chen Yan , Zhang Yongfang , Huang Yousheng , Xie Na 
Published: January 2023

Abstract
The role of circular RNAs (ciRNAs; whose dysregulation causes various cancer types) in gastric cancer (GC) remains largely unknown. Here, we investigated the expression of a ciRNA, hsa_circ_0000026 (ciR-0026), in GC tissues and deciphered the molecular mechanism by which ciR-0026 suppresses GC cell proliferation. ciR-0026 expression in GC cells was analyzed using quantitative PCR. The clinical significance of the changes in ciR-0026 expression in GC cells was analyzed using the SPSS.25 software. The effects of ciR-0026overexpression on GC cell phenotypes were determined using colony formation, cell counting kit-8, and transwell assays. Additionally, biotin-coupled probe RNA pull-down and dual-luciferase reporter assays were performed. ciR-0026 expression was downregulated in 90.5% (57/63) of the primary GC tissues compared with that in the adjacent gastric mucosal tissues (p< 0.05). The expression of ciR-0026 was affected by tumor differentiation, lymph node metastasis, and tumor node metastasis stage (p < 0.05). An increase in ciR-0026 expression suppressed GC cell proliferation and invasion in vitro. Mechanistically, ciR-0026 acted as a sponge for microRNA (miR)-23a-5p, which directly targeted the phosphate and tension homology deleted on chromosome ten (PTEN) gene. The proliferative, invasive, and migratory abilities of GC cells were inhibited by ciR-0026 upregulation. Moreover, high ciR-0026 levels inhibited epithelial-to-mesenchymal transition of GC cells via the modulation of the miR-23a-5p/PTEN axis; however, suppressing PTENexpression reversed these effects. In conclusion, ciR-0026 may be a potential prognostic biomarker of GC, serving as a suppressor of GC via the miR-23a-5p/PTEN axis.
Diazotrophic endophyte Klebsiella pneumoniae N5 from sorghum show cross-colonization and plant growth promotion in Rice
Authors: Gauswami Ashvin , Jha Sanjay , Patel Swati , Suthar Harish , Parekh Vipul , Jajda Harsur 
Published: January 2023

Abstract
Biological N2 fixation (BNF) by endophytic diazotrophic bacteria is a spontaneous course of action where soil N is limited. A Sorghum endophytic bacterium “N5” having highest potential of nitrogen fixation, was isolated and characterized. The strain N5 was screened out by PCR amplification of the nifH gene and acetylene reduction assay of nitrogenase activity. Morphological, biochemical and 16S rDNA sequencing analyses identified the strain N5 as Klebsiella pneumoniae. The strain N5 also has other plant growth-promoting (PGP) potentials, such as phosphate solubilization, production of indole acetic acid, siderophore and ammonia, and anti-fungal activity. PGP endophyte K. pneumoniae N5 was inoculated onto rice seedlings to examine its interaction under axenic conditions and to characterize its ability to colonize non-native host plant rice. Colonization of rice root was confirmed by electron microscopy. Increase in shoot length, root length, dry and fresh weight of root and shoot, chlorophyll contents and nitrogen content of the plant and soil were observed in the K. pneumoniae N5 treated plants as compared to the untreated plants which ultimately helps to improve growth and yield of rice. Our finding expends the horizon of endophytic Klebsiella pneumoniae from sorghum (C4) to rice (C3) plant.
A smart emergency system: Case of COVID-19
Authors: Frikha Hounaida , kamoun-Abid Ferdaous , Meddeb-Makhoulf Amel , Zarai Faouzi 
Published: January 2023

Abstract
In the case of an epidemic, the transfer of patients to emergency departments and their physiological parameters remains the major challenge in this critical situation. Rapid and effective diagnosis can save the lives of these patients. This goal is achieved by using deep learning in classifying information into emergencies to send it first. To do this, we propose in this article, an intelligent emergency system for the rapid transmission of information from emergency patients to the hospital that has the best health care for these patients using a deep learning approach. First, the fusion method combines data collected and filtered from patients' electronic medical records with data captured by the wireless medical sensor network during the transport phase. Next, the method of selecting the parameters is used as inputs to the learning model. The information collected and learning outputs, such as emergency alerts are sent via Wi-Fi and 5G equipment in our intelligent system. Accuracy of our proposed contribution reaches 98% with 1.53s run time. This finding shows that our system is effective and is used in cases of epidemics as COVID-19.
Combined exposure to high-cholesterol diet and PM 2.5 : Brain injury and regulatory mechanism of HIF-1α in female mice
Authors: Chen Wenqi , Chen Shanshan , Bai Lirong , Zhao Lifang , Zhang Mei , Dong Chuan , Yang Zhu , Song Yuanyuan , Yung Ken Kin Lam , Cai Zongwei , Li Ruijin 
Published: January 2023

Abstract
High-cholesterol diet (HCD) and fine particulate matter (PM2.5) are related to stroke. The comprehensive influence of two factors on stroke is not well known, especially for females. In this study, we investigated brain injury and behavioral changes in female mice exposed to HCD plus PM2.5 for three months and six months, respectively. We measured gene expression of some factors related to stroke and the blood-brain barrier (BBB) in different groups of mice and further explored molecular mechanisms of epigenetic regulation of hypoxia-inducible factor-1α (HIF-1α). The results showed that HCD and PM2.5 co-exposure altered brain-body weight ratio, induced brain pathology damage and behavioral abnormality and elevated inflammatory markers (IL-6 and TNF-α) in mice relative to exposure to PM2.5 or HCD alone. Six-month exposure caused more severe brain damage than that three-month exposure. Co-exposure significantly changed the expressions of HIF-1α and the key genes (VEGF/COX-2/MMP-9/ZO-1/Occludin) in the HIF-1α signaling pathway in the brains of mice compared to the single exposure. Interestingly, HIF-1α was epigenetic regulated by histone deacetylase 4 (HDAC4) and histone 3 lysine 9 acetylation (H3K9ac) by chromatin immunoprecipitation (ChIP) assay. It suggests that the HIF-1α pathway exerts a crucial regulatory role in brain injury and behavioral abnormality in female mice after six-month exposure to HCD plus PM2.5, which are potential mechanisms for HCD and PM2.5-triggering stroke risk in female individuals.
Monitoring and optimization of machining process when turning of AISI316L based on response surface methodology, artificial neural network and desirability function.
Authors: benkhelifa oussama , Cherfia Abdelhakim , Nouioua Mourad 
Published: January 2023

Abstract

The object of this research is to investigate the effect of cutting parameters such as cutting speed (Vc), feed rate (f) and depth of cut (ap) on machining parameters including cutting temperature (TC) and tool flank wear (VB) during dry turning of AISI 316L using coated carbide tool. The experiments were conducted according to Taguchi L27 orthogonal array, RSM and ANN have been used. Results revealed that (ap) found to be the dominant factor for TC. VB mainly influenced by Vc, f and ap, respectively. The prediction results obtained by ANN and RSM models showed a good agreement with experimental data. However, ANN models proved their capability to provide more accurate results compared to RSM models. According to the optimization analysis, Desirability function showed good accuracy in optimization.
The association of isocarbophos and isofenphos with different types of glucose metabolism: the role of inflammatory indicators Running Title: Isocarbophos, isofenphos, inflammatory indicators, and glucose metabolism
Authors: Geng Jintian , Wei Dandan , Wang Lulu , Xu Qingqing , Wang Juan , Shi Jiayu , Ma Cuicui , Zhao Mengzhen , Huo Wenqian , Jing Tao , Wang Chongjian , Mao Zhenxing 
Published: January 2023

AbstractAims Our investigation focused on the associations between isocarbophos and isofenphos with impaired fasting glucose (IFG) and type 2 diabetes mellitus (T2DM), as well as how much of these associations might be accounted for by markers of inflammation. Methods There were 2701 participants in a case-control study. Plasma isocarbophos and isofenphos concentrations were measured using gas chromatography and triple quadrupole tandem mass spectrometry. Generalized linear models were used to calculate the relationships between plasma isofenphos and isocarbophos levels with inflammatory factor levels and T2DM. Inflammatory indicators were used as mediators to estimate the mediating effects on the above associations. Results Isocarbophos and isofenphos were positively related with T2DM after adjusting for other factors. The odds ratio (OR) (95% confidence interval (CI)) for T2DM was 4.1% (OR (95% CI): 1.041 (1.015, 1.068)) and the odds ratio (95% CI) for IFG was 6.6% (OR (95% CI): 1.066 (1.009, 1.127)) per unit rise in ln-isocarbophos. The incidence of T2DM increased by 6.4% for every 1 unit more of ln-isofenphos (OR (95% CI): 1.064 (1.041, 1.087)). Additionally, a 100% rise in ln-isocarbophos was linked to 3.3% higher ln-HOMA2IR and a 0.029 mmol/L higher glycosylated hemoglobin A1c (HbA1c) (95% CI: 0.007, 0.051). While a 100% rise in ln-isofenphos was linked to increases in ln-HOMA2 (95% CI: 1.6%, 5.2%) and ln-HOMA2IR (95% CI: 3.6%, 8.1%) of 5.8% and 3.4%, respectively. Furthermore, white blood cell (WBC) and neutrophilic (NE) were found to be mediators in the relationship between isocarbophos and T2DM, and the corresponding proportions were 17.12% and 17.67%, respectively. Conclusion Isofenphos and isocarbophos are associated with IFG and T2DM in the rural Chinese population, and the inflammatory indicators (WBC and NE) have a significant role in this relationship.
Intriguing physicochemical properties of N-doped Graphene oxide based ZnS nanowires for photocatalytic and biomedical applications
Authors: Dake D. V. , Raskar N. D. , Mane V. A. , Sonpir R. B. , Stathatos E. , Mutta V. , Meena R. , Asokan K. , Dole B. N. 
Published: January 2023

Abstract
Superparamagnetic N-doped graphene oxide (GO)- with ZnS nanowires was synthesized by a one-step hydrothermal method by doping dilute amounts of Ga, Cr, In, and Al ions for water treatment and biomedical applications. In these experiments, to enhance their properties, 2% of Ga3+, In3+, and or Al3+ were codoped along with 2% Cr ions in these ZnS nanowires. The nanocomposite with the composition, In0.02Cr0.02Zn0.96S, has better photocatalytic efficiency than other co-doped nanocomposites. The In (metalloids) and Cr (transition metal ion) are the best combinations to increase the magnetic properties which are beneficial for photocatalytic activity. Synthesized nanocomposite materials were characterized by several techniques such as X-ray diffraction, Field emission-scanning electron microscope (FESEM) with EDAX, vibrating sample magnetometer (VSM), UV-Vis, X-ray photoelectron spectroscopy (XPS), and fluorescence spectroscopy. The correlation of intriguing magnetic properties with their photocatalytic properties is also discussed. XPS was employed for the detection of surface defects, phase transformation, and the nature of chemical components present in the nanocomposites. The Frankel and substitutional defects have a direct impact on photocatalytic activity that was determined from the fluorescence (FL) spectroscopy. FL and XPS reveal that the Cr and In codoped composite has a higher percentage of defects hence its photocatalytic efficiency reaches 94.21%.
Imaging Quality Improvement for Wavefront Colding System With Radially Symmetrical Phase Mask
Authors: Le Vannhu 
Published: January 2023

Abstract
In this paper, we introduce the novel method to improve the image quality of the optical imaging system with the radially symmetrical phase masks. By introduced a 0-π phase mask into the radially symmetrical phase mask, the new phase mask is generated, which produces the dark-shape point spread function. Next, by rotating this phase mask with the amplitude mask for different angles, the point spread function is rotated by the same angle. After, the digital processing is used to restore the high-quality image. The simulation results demonstrated the effectiveness of the proposed method in comparison to the previously radially symmetrical phase mask.
Impact of the Affordable Care Act on participation in the Supplemental Nutrition Assistance Program among low-income older Medicare beneficiaries
Authors: Kim Hyunmin , Mahmood Asos , Chang Cyril F. , Hammarlund Noah E. , Dobalian Aram 
Published: January 2023

Abstract
Background: The Affordable Care Act (ACA) provisions, especially Medicaid expansion, in the United States (US) are believed to have spillover effects, such as boosting the Supplemental Nutrition Assistance Program (SNAP) participation among eligible individuals. However, little is known about the impact of the ACA, with its focus on the dual eligible population, on SNAP participation. The current study investigates whether the ACA, under an explicit policy aim of enhancing the interface between Medicare and Medicaid, has improved participation in the SNAP among low-income older Medicare beneficiaries.
Methods: We extracted data from 2009 through 2018 cycles of the US Medical Expenditure Panel Survey (MEPS) for low-income (≤%138 Federal Poverty Level [FPL]) older Medicare beneficiaries (n=50,466; aged ≥65), and low-income (≤%138 FPL) younger adults (aged 20 to <65 years, n=190,443). MEPS respondents of >%138 FPL incomes, younger Medicare and Medicaid beneficiaries, and older adults without Medicare were excluded from this study. Using a quasi-experimental comparative interrupted time-series design, we examined (1) whether ACA’s support for the Medicare-Medicaid dual-eligible program, through facilitating the online Medicaid application process, was associated with an increase in SNAP uptake among low-income older Medicare beneficiaries, and (2) in the instance of an association, to assess the magnitude of SNAP uptake that can be explicitly attributed to the policy implementation. The outcome, SNAP participation, was measured annually from 2009 through 2018. The year 2014 was set as the intervention point when the Duals Office started facilitating Medicaid applications online for eligible Medicare beneficiaries.
Results: Overall, a change in the probability of SNAP enrollment from the pre- to post-intervention period was 17.4 percentage points higher in low-income older Medicare enrollees, compared to the similarly low-income, SNAP-eligible, younger adults (β=0.174, P<.001). This boost in SNAP uptake was significant and more apparent among older White (β=0.137, P=.049) and Asian (β=0.408, P=.047) adults.
Conclusions: The ACA had a positive, measurable effect on SNAP participation among older Medicare beneficiaries. Policymakers should consider additional approaches that link enrollment to multiple programs to increase SNAP participation. Further, there may be a need for additional, targeted efforts to address structural barriers to uptake among African Americans.
Effect of waiting time on patients’ satisfaction in outpatient: An empirical investigation
Authors: Zhang Hui , Ma Weimin , Zhou Shufen , Zhu Jingjing , wang li , Zhang Man 
Published: January 2023

Abstract
Study objective

This study mainly aimed to identify the effect of waiting times on patient satisfaction.

Methods

A clever questionnaire was designed, and it contained two parts. The first part includes a series of short, standardized questions about expected waiting time (EWT), reasonable waiting time (RWT), limited waiting time (LWT), and basic personal information. This part of the questionnaire was filled in when the patient entered the waiting area. The second part includes perceived waiting time (PWT) and satisfaction evaluation, and this part was filled before patients entered the doctor’s office. The two questionnaires were summarized by the same number. The actual waiting time (AWT) was calculated based on the time difference between the registration time recorded by the experimenter and the time between entering the clinic. Linear regression was used to analyze the influence of waiting times on satisfaction. Before starting data collection, this study was approved by the hospital health ethics committee.

Results

A total of 323 questionnaires were collected, in which 292 (90.4%) were valid. EWT, LWT, RWT, and PWT had a significant effect on patient’s satisfaction (p = 0.006, p = 0.043, p = 0.009, p = 0.000), whereas AWT had no significant effect on satisfaction (p = 0.365). The difference between EWT and AWT had a significant effect on satisfaction (p = 0.000), while the difference between PWT and AWT had a significant effect on satisfaction (p = 0.000). Age, education background, gender, appointment, and hospital visit experience had no significant effect on patient satisfaction (p = 0.105, p = 0.443, p = 0.260, p = 0.352, p = 0.461).

Conclusions

The patient’s satisfaction of waiting time was not directly affected by the AWT, but by the subjective waiting times. And the difference between AWT and EWT has a significant impact on patient’s satisfaction. Meanwhile, the difference between AWT and PWT also has a significant impact on patient’s satisfaction. Therefore, service quality can be improved by hospital managers by focusing on the adjustment of patients’ subjective waiting time while reducing the objective waiting time.
Monitoring the effectiveness of a bio-fertilizer restoration technique using multi-resolution satellite and meteo-data: the case of Xylella fastidiosa subsp. Pauca
Authors: Blonda Palma , Tarantino Cristina , Scortichini Marco , Tarantino Maria , Adamo Maria 
Published: January 2023

Abstract
Xylella fastidiosa, subsp. pauca (Xfp), has attacked the olive trees in Southern Italy causing severe impacts on the agro-ecosystem services, such as provisioning, regulating and cultural. To reduce both the Xfp cell concentration and the symptom disease, a bio-fertilizer restoration technique has been used. Our study applied multi-resolution satellite data to evaluate the effectiveness of such restoration action at both field and tree scale. For field scale, a time series of High Resolution (HR) Sentinel-2 images, acquired in the months of July and August from 2015 to 2020, was employed. First, four spectral indices from treated and untreated fields were compared. Then, their trends were correlated to meteo-events. For tree-scale, Very High Resolution (VHR) Pléiades images were selected at the closest dates of the Sentinel-2 data to investigate the response to treatments of each different cultivar. All indices from HR and VHR images resulted higher in treated fields than in those untreated. The analysis of VHR indices revealed different cultivar response to treatments. The findings from HR data could be used to evaluate plant conditions at field level after treatments, while VHR imagery could be used to optimize treatment doses per cultivar. The findings in agreement with in-field PCR results.
Doubling the equatorial for the prescribed scalar curvatureproblem on ${\mathbb{S}}^N$
Authors: Duan Lipeng , Musso Monica , Wei Suting 
Published: January 2023

Abstract
Note: Please see pdf for full abstract with equations.
We consider the prescribed scalar curvature problem on SN
ΔSN v −N(N − 2)/2 v + ​​K̃ (y)v N+2/N−2 = 0 on SN, v > 0 in SN,
under the assumptions that the scalar curvature ​K̃ is rotationally symmetric, and has a positive local maximum point between the poles. We prove the existence of infinitely many non-radial positive solutions, whose energy can be made arbitrarily large. These solutions are invariant under some non-trivial sub-group of O(3) obtained doubling the equatorial. We use the finite dimensional Lyapunov-Schmidt reduction method.
Heavy metal(loid)s contamination, pollution quantification, and health risk assessment along with coal mines in the Hangu and Kurram Districts, Northwest Pakistan
Authors: Din Imran Ud , Muhammad Said , Faisal Shah , Rehman Inayat ur , Ali Wajid 
Published: January 2023

Abstract

Heavy metal(loid)s (HM) was considered hazardous contaminants owing to their toxicity, persistency, and bio-accumulative nature in the environment. The current study aimed to examine HM concentrations in coal, coal dust, and surrounding soil along with coal mines in the Hangu and Kurram Districts. Using an Atomic Absorption Spectrophotometer, samples of coal, coal dust, and soil were analyzed for HM concentrations. Coal, coal dust, and soil showed maximum mean concentrations of 62100, 52610, and 45300 mg/kg for iron (Fe) and a minimum of 1.14, 0.9, and 2.0 mg/kg for molybdenum (Mo), respectively. HM concentrations in coal dust and soil were evaluated for contamination factor (CF), ecological risk index (ERi), potential ecological risk index (PERI), and human risk assessment. CF indicated very high contamination (CF > 6) in coal dust 6.05 and soil 5.2 of the Hangu District for cadmium (Cd). The ERi data showed that most HM in coal dust and soil of studied districts exhibited low ecological risk (ERi < 40), except for Cd. Moreover, the coal dust and soil of Hangu District showed considerable ecological risk (190 < PERI < 380), while the coal dust of Kurram District exhibited moderate ecological risk (95 < PERI < 190). Among human exposure pathways, ingestion was the primary exposure route compared to other routes (inhalation and dermal). The non-carcinogenic risk, i.e., hazard quotient (HQ), was observed in the order of; ingestion > dermal > inhalation in humans. The hazard index (HI) values were found to be higher in children (HI > 1) than in adults via coal dust and soil and could cause chronic health problems. Cancer risk (CR) values were noted within the US EPA threshold. Therefore, the current study recommends regular monitoring of HM in the area to control the health hazard to residents in the future.
The peanut root exudate increases the transport and metabolism of nutrients and enhances the plant growth-promoting effects of Burkholderia pyrrocinia strain P10
Authors: Han Lizhen , Zhang Hong , Bai Xue , Jiang Biao 
Published: January 2023

Abstract
Background: Burkholderia pyrrocinia strain P10 is a plant growth-promoting rhizobacterium (PGPR) that can substantially increase peanut growth. However, the mechanisms and pathways involved in the interaction between B. pyrrocinia P10 and peanut remain unclear. To clarify complex plant–PGPR interactions and the growth-promoting effects of PGPR strains, the B. pyrrocinia P10 transcriptome changes in response to the peanut root exudate (RE) were elucidated and the effects of RE components on biofilm formation and IAA secretion were analyzed.
Results: During the early interaction phase, the peanut RE enhanced the transport and metabolism of nutrients, including carbohydrates, amino acids, nitrogen, and sulfur. Although the expression of flagellar assembly-related genes was down-regulated, the expression levels of other genes involved in biofilm formation, quorum sensing, and Type II, III, and VI secretion systems were up-regulated, thereby enabling strain P10 to outcompete other microbes to colonize the peanut rhizosphere. The peanut RE also improved the plant growth-promoting effects of strain P10 by activating the expression of genes associated with siderophore biosynthesis, IAA production, and phosphorus solubilization. Additionally, organic acids and amino acids were identified as the dominant components in the peanut RE. Furthermore, strain P10 biofilm formation was induced by malic acid, oxalic acid, and citric acid, whereas IAA secretion was promoted by the alanine, glycine, and proline in the peanut RE.
Conclusions: The peanut RE positively affects B. pyrrocinia P10 growth, while also enhancing colonization and growth-promoting effects during the early interaction period. These findings may help to elucidate the mechanisms underlying complex plant–PGPR interactions, with potential implications for improving the applicability of PGPR strains.