bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical utility of overviews on adverse events of pharmacological interventions
Authors: Sachse Thilo , Kanji Salmaan , Thabet Pierre , Schmiedl Sven , Thürmann Petra , Guirguis Fadi , Sajwani Shellyza , Gauthier Marie-France , Lunny Carole , Mathes Tim , Pieper Dawid 
Published: July 2023

Abstract
Background
Overviews (i.e., systematic reviews of systematic reviews, meta-reviews, umbrella reviews) are a relatively new type of evidence synthesis. Among others, one reason to conduct an overview is to investigate adverse events (AEs) associated with a healthcare intervention. Overviews aim to provide easily accessible information for healthcare decision-makers including clinicians. We aimed to evaluate the clinical utility of overviews investigating AEs.

Methods
We used a sample of 27 overviews exclusively investigating drug-related adverse events published until 2021 identified in a prior project. We defined clinical utility as the extent to which overviews are perceived to be useful in clinical practice. Each included overview was assigned to one of seven pharmacological experts with expertise on the topic of the overview. The clinical utility and value of these overviews were determined using a self-developed assessment tool. This included four open-ended questions and a ranking of three clinical utility statements completed by clinicians. We calculated frequencies for the ranked clinical utility statements and coded the answers to the open-ended questions using an inductive approach.

Results
The overall agreement with the provided statements was high. According to the assessments, 67% of the included overviews generated new knowledge. In 93% of the assessments, the overviews were found to add value to the existing literature. The overviews were rated as more useful than the individual included systematic reviews (SRs) in 85% of the assessments. The answers to the open-ended questions revealed two key aspects of clinical utility in the included overviews. Firstly, it was considered useful that they provide a summary of available evidence (e.g., along with additional assessments, or across different populations, or in different settings that have not been evaluated together in the included SRs). Secondly, it was found useful if overviews conducted a new meta-analysis to answer specific research questions that had not been answered previously.

Conclusions
Overviews on drug-related AEs are considered valuable for clinical practice by clinicians. They can make available evidence on AEs more accessible and provide a comprehensive view of available evidence. As the role of overviews evolves, investigations such as this can identify areas of value.
Can blockchain technology strengthen consumer preferences for credence attributes?
Authors: Contini Caterina , Boncinelli Fabio , Piracci Giovanna , Scozzafava Gabriele , Casini Leonardo 
Published: July 2023

AbstractConsumers’ interest in product information, such as nutrition, environment, and social aspects, is increasing in the food market. Blockchain technology can enhance credibility of quality signals on labels through transparency and accountability in the supply chain. This study examines the impact of blockchain technology on reducing consumer uncertainty about credence attributes and facilitating informed choices. It is particularly relevant for food policies, as greater consumer awareness promotes proactive involvement in sustainability and public health. We conducted a choice experiment with 300 Italian consumers, focusing on craft beer and analysing how blockchain technology in strengthens organic and DOP certification. The results provide valuable insights for producers and policymakers to develop voluntary approaches that engage society in objectives that concern the collective well-being. They highlight the potential of blockchain technology in communicating credence attributes and empowering consumers. However, our study reveals that the impact of blockchain technology on choices is influenced by a different level of trust, likely due to the complexity of understanding its functioning.
Sodium levels and grazing pressure shape natural communities of the intracellular pathogen Legionella
Authors: Bergman Oded , Be’eri-Shlevin Yaron , Ninio Shira 
Published: July 2023

Abstract
Background
Legionella are parasites of freshwater protozoa, responsible for Legionellosis. Legionella can be found in a variety of aquatic environments, including rivers, lakes, and springs, as well as in engineered water systems where they can potentially lead to human disease outbreaks. Legionella are considered to be predominantly freshwater organisms with a limited ability to proliferate in saline environments. Exposure of Legionella to high sodium concentrations inhibits growth and virulence of laboratory strains, particularly under elevated temperatures. Nonetheless, Legionella have been identified in some saline environments where they likely interact with various protozoan hosts. In this work, we examine how these selection pressures, sodium and grazing, help shape Legionella ecology within natural environments. Utilizing Legionella-specific primers targeting a variable region of the Legionella 16S rRNA gene, we characterized Legionella abundance, diversity, and community composition in natural spring clusters of varying sodium concentrations, focusing on high sodium concentrations and elevated temperatures.

Results
We observed the highest abundance of Legionella in spring clusters of high salinity, particularly in combination with elevated temperatures. Legionella abundance was strongly related to sodium concentrations. The Legionella community structure in saline environments was characterized by relatively low diversity, compared to spring clusters of lower salinity. The community composition in high salinity was characterized by few dominant Legionella genotypes, not related to previously described species. Protozoan microbial community structure and composition patterns resembled those of Legionella, suggesting a common response to similar selection pressures. We examined Legionella co-occurrence with potential protozoan hosts and found associations with Ciliophora and Amoebozoa representatives.

Conclusions
Our results indicate that selection forces in saline environments favor a small yet dominant group of Legionella species that are not closely related to known species. These novel environmental genotypes interact with various protozoan hosts, under environmental conditions of high salinity. Our findings suggest that alternative survival mechanisms are utilized by these species, representing mechanisms distinct from those of well-studied laboratory strains. Our study demonstrate how salinity can shape communities of opportunistic pathogens and their hosts, in natural environments, shedding light on evolutionary forces acting within these complex environments.
Using a drifting GPS-Argos satellite buoy as a method for detecting acoustic-tagged fish offshore in an ancient lake
Authors: Yoshida Makoto A. , Mabuchi Kohji 
Published: July 2023

Abstract
Background
Telemetry methods that allow low-cost, offshore tracking are required for the effective conservation and sustainable use of migratory fish in large lakes with vast and deep offshore areas. Surface drifting buoys, or drifters, are promising platforms for acoustic receiver attachment that have few operational constraints. However, the low recovery rate of drifters in the open ocean presents a challenge. In contrast, closed waters, such as lakes, are more suitable for operating drifters since drifting objects eventually strand on the shore. In this study, we examined the effectiveness of a single GPS-tracked drifter for telemetry surveys of the common carp Cyprinus carpio, which migrates throughout the large offshore area of a large lake, Lake Biwa, in Japan. Its performance was assessed regarding the success rate of drifter recovery, detection frequency of tagged individuals, search area of the drifter, and accuracy of detection locations.

Results
We successfully recovered the drifter during all eight deployments. In 6 deployments, tagged individuals were detected 777 times. The drifter successfully detected 48% (10 of 21) of the tagged individuals, exhibiting higher performance (10–33%) than fixed receivers used in other telemetry studies conducted on highly migratory fish species in the lake. The drifter scanned an area of 5.5–8.0 km2 per day. Based on the data obtained from the field trials, the horizontal location accuracy of detected individuals was estimated to be approximately 400 m in shallow coastal areas and 600 m in deep offshore areas. Furthermore, the horizontal distance of tagged individuals traveled was estimated to be between 2 and 9 km within a day.

Conclusion
Our newly developed method deploys and retrieves a single GPS drifter that allows low-cost, wide-ranging telemetry surveys in offshore areas of large lakes. The lack of boat noise, moderate moving speed, and continuity of observation range of the drifter seemingly enabled the efficient detection of tagged individuals. Thus, the drifter method offers a promising novel approach for acoustic telemetry in offshore waters that can operate in combination with large receiver arrays and other mobile platforms.
Mental illness stigma among indigenous communities in Bangladesh: a cross-sectional study
Authors: Faruk Md. Omar , Rosenbaum Simon 
Published: July 2023

Abstract
Background
Mental illnesses stigma is a universal and transcultural phenomenon. While mental illnesses stigma is pervasive in Bangladesh, very little research exists on stigma toward mental illnesses among indigenous communities. This study aimed to investigate the prevailing stigma and the risk factors among different indigenous communities in the Chattogram Hill Tracts (CHT) in Bangladesh.

Methods
A cross-sectional survey was carried out and participants were recruited purposively from Rangamati, a South-Eastern district of Bangladesh in the CHT. Participants from various indigenous communities including Chakma, Marma, Rakhine, Tripura, and Pangkhua were recruited. The 28- item Bangla translated version of the Mental Illnesses Stigma Scale was used. Independent-samples t-test, ANOVA, and multiple regression were performed.

Results
The results indicate evidence of a gender difference with females reporting more stigma than their male counterparts. Age, gender, socioeconomic status, and monthly income are associated with stigma among indigenous people. Further analyses of the subscales indicated significant differences among sociodemographic variables.

Conclusions
The results provide an insight into the prevailing stigma and associate risk factors among indigenous communities. The results may help inform anti-stigma interventions targeting indigenous communities in Bangladesh.
The marriage of immunomodulatory, angiogenic, and osteogenic capabilities in a piezoelectric hydrogel tissue engineering scaffold for military medicine
Authors: Wu Ping , Shen Lin , Liu Hui-Fan , Zou Xiang-Hui , Zhao Juan , Huang Yu , Zhu Yu-Fan , Li Zhao-Yu , Xu Chao , Luo Li-Hua , Luo Zhi-Qiang , Wu Min-Hao , Cai Lin , Li Xiao-Kun , Wang Zhou-Guang 
Published: July 2023

Abstract
Background
Most bone-related injuries to grassroots troops are caused by training or accidental injuries. To establish preventive measures to reduce all kinds of trauma and improve the combat effectiveness of grassroots troops, it is imperative to develop new strategies and scaffolds to promote bone regeneration.

Methods
In this study, a porous piezoelectric hydrogel bone scaffold was fabricated by incorporating polydopamine (PDA)-modified ceramic hydroxyapatite (PDA-hydroxyapatite, PHA) and PDA-modified barium titanate (PDA-BaTiO3, PBT) nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. The physical and chemical properties of the Cs/Gel/PHA scaffold with 0–10 wt% PBT were analyzed. Cell and animal experiments were performed to characterize the immunomodulatory, angiogenic, and osteogenic capabilities of the piezoelectric hydrogel scaffold in vitro and in vivo.

Results
The incorporation of BaTiO3 into the scaffold improved its mechanical properties and increased self-generated electricity. Due to their endogenous piezoelectric stimulation and bioactive constituents, the as-prepared Cs/Gel/PHA/PBT hydrogels exhibited cytocompatibility as well as immunomodulatory, angiogenic, and osteogenic capabilities; they not only effectively induced macrophage polarization to M2 phenotype but also promoted the migration, tube formation, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) and facilitated the migration, osteo-differentiation, and extracellular matrix (ECM) mineralization of MC3T3-E1 cells. The in vivo evaluations showed that these piezoelectric hydrogels with versatile capabilities significantly facilitated new bone formation in a rat large-sized cranial injury model. The underlying molecular mechanism can be partly attributed to the immunomodulation of the Cs/Gel/PHA/PBT hydrogels as shown via transcriptome sequencing analysis, and the PI3K/Akt signaling axis plays an important role in regulating macrophage M2 polarization.

Conclusion
The piezoelectric Cs/Gel/PHA/PBT hydrogels developed here with favorable immunomodulation, angiogenesis, and osteogenesis functions may be used as a substitute in periosteum injuries, thereby offering the novel strategy of applying piezoelectric stimulation in bone tissue engineering for the enhancement of combat effectiveness in grassroots troops.
A symbiotic footprint in the plant root microbiome
Authors: Hartman Kyle , Schmid Marc W. , Bodenhausen Natacha , Bender S. Franz , Valzano-Held Alain Y. , Schlaeppi Klaus , van der Heijden Marcel G.A. 
Published: July 2023

Abstract
Background
A major aim in plant microbiome research is determining the drivers of plant-associated microbial communities. While soil characteristics and host plant identity present key drivers of root microbiome composition, it is still unresolved whether the presence or absence of important plant root symbionts also determines overall microbiome composition. Arbuscular mycorrhizal fungi (AMF) and N-fixing rhizobia bacteria are widespread, beneficial root symbionts that significantly enhance plant nutrition, plant health, and root structure. Thus, we hypothesized that symbiont types define the root microbiome structure.

Results
We grew 17 plant species from five families differing in their symbiotic associations (no symbioses, AMF only, rhizobia only, or AMF and rhizobia) in a greenhouse and used bacterial and fungal amplicon sequencing to characterize their root microbiomes. Although plant phylogeny and species identity were the most important factors determining root microbiome composition, we discovered that the type of symbioses also presented a significant driver of diversity and community composition. We found consistent responses of bacterial phyla, including members of the Acidobacteria, Chlamydiae, Firmicutes, and Verrucomicrobia, to the presence or absence of AMF and rhizobia and identified communities of OTUs specifically enriched in the different symbiotic groups. A total of 80, 75 and 57 bacterial OTUs were specific for plant species without symbiosis, plant species forming associations with AMF or plant species associating with both AMF and rhizobia, respectively. Similarly, 9, 14 and 4 fungal OTUs were specific for these plant symbiont groups. Importantly, these generic symbiosis footprints in microbial community composition were also apparent in absence of the primary symbionts.

Conclusion
Our results reveal that symbiotic associations of the host plant leaves an imprint on the wider root microbiome – which we term the symbiotype. These findings suggest the existence of a fundamental assembly principle of root microbiomes, dependent on the symbiotic associations of the host plant.
Relationship between disability and psychiatric outcome in multiple sclerosis patients and its determinants
Authors: Hassan Shady Safwat , Darwish Esam S. , Ahmed Gellan K. , Azmy Samah R. , Haridy Nourelhoda A. 
Published: July 2023

Abstract
Background
Multiple sclerosis (MS) is an inflammatory demyelinating central nervous system disease with diverse clinical manifestations. The present study aimed to compare the psychiatric outcomes of MS patients with full ambulatory versus impaired ambulatory function and identify the potential risk factors for disability in MS. Seventy MS patients were classified into two groups based on their Expanded Disability Status Scale (EDSS) scores, Group A: full ambulatory (EDSS ≤ 4.5) (N = 48), Group B: impaired ambulatory (EDSS ≥ 5) (N = 22). All participants were evaluated by the Socioeconomic Scale, Hamilton Anxiety Scale, Hamilton Depression Scale, Brief Psychiatric Rating Scale, and The Pittsburgh Sleep Quality Index.

Results
In the total cohort (N = 70), females represented (77.1%). The mean age was 31.16 ± 6.46, the mean age of onset was 26 ± 6.083, and the mean disease duration was 5.33 ± 3.653 years which was less in Group A than in Group B. Relapsing–remitting multiple sclerosis (RRMS) was the most common presentation (80%), representing 93.6% of Group A. Group A reported more severe depression and anxiety, while Group B had more poor sleep quality. Correlation analysis showed increased relapses, progressive-relapsing multiple sclerosis (PRMS), cervical or dorsal plaques, sensory or motor manifestations, and precipitancy increased disability, while RRMS type decreased disability.

Conclusions
Full ambulatory MS patients had high anxiety and depression, while impaired ambulatory MS patients had poor sleep quality. Associated factors for disability were frequent relapses, plaque location, MS subtype, sphincter, and sensory symptoms.
Trial registration clinicaltrials.gov, NCT05029830. Registered: September 01, 2021, https://clinicaltrials.gov/ct2/show/NCT05029830
Willingness to receive COVID-19 vaccine in relation to psychological wellbeing among a sample of university students in Vietnam
Authors: Bui Nhat-Le , Thi Hue Vu , Suong Mai Vu Ngoc , Thi Yen Vy Nguyen , Le Thuy-Tien , Thi Lan-Anh Nguyen , Tang Thuy-Linh , Sharma Rajesh , Chu Dinh-Toi 
Published: July 2023

Abstract
Background and aims
Nowadays, there are contradicting opinions about the benefits and risks of COVID-19 vaccine, which lead to the hesitance among recipients to receive the vaccine. This study aims to evaluate the university student’s attitude toward COVID-19 vaccine, preventative measure, and status of their psychological wellbeing.

Methods
This was a cross-sectional study among Vietnamese students aged ≥ 18 years to evaluate their willingness to receive COVID-19 vaccine, psychological wellbeing status, and preventive measure compliance. The participants were asked to fill in a questionnaire via Google Form. Descriptive and analytical statistics were performed with p < 0.05 being considered as statistically significant.

Results
A total of 2998 randomly selected participants in Vietnam completed the questionnaire with participants’ mean age of 20.26 $$\pm$$
±
1.84. The mean score for the willingness to receive COVID-19 vaccination was 46.13 $$\pm$$
±
19.03 (the highest point was 54). Regarding preventative measures, the mean score of compliance was 7.15 $$\pm$$
±
1.98 over a 10 points scale and “being vaccinated or not” did not affect their compliance. 56.94% participants were having normal psychological wellbeing at the time of survey and rest 43.06% reported to have experienced stress, varying from slight to severe level. There might be a trend that those studying medicine or those with higher educational level had higher rate of stress than others.

Conclusion
The study showed a positive result of Vietnamese students’ willingness to receive COVID-19 vaccine and compliance towards preventive measures. Besides, we also reported that almost half participants had experienced stress during the pandemic. Developing proper interventions to deal with factors contributing to psychological wellbeing and related problems are essential to deal with and overcome the pandemic or post-pandemic struggles.
Propionic acidaemia crisis: a specific DWI finding—case report
Authors: Abdella Khairy 
Published: July 2023

Abstract
Background
Propionic acidaemia is among the rarest metabolic disorders described in the literatures. It has resulted from an inborn error in the catabolism of amino acids with an accumulation of organic acids as a consequence of this error. This illness was especially manifested and discovered in early postnatal life with other forms seen later in the childhood period.
It has different clinical presentations with poor feeding, hypotonia, and lethargy described in the early neonatal period, while mental retardation, global developmental delay, and seizures are seen in childhood.

Case presentation
We present a case of a 4-month-old child already diagnosed in his early neonatal period with propionic acidaemia currently presented with propionic acidaemic crisis in the form of frequent attack of seizures and severe hyperammonemia with unique DWI features which shows bilateral symmetrical restricted diffusion in the subcortical and subinsular white matter that are specific to propionic acidaemia crisis and does not relate to any vascular territory.

Conclusion
Propionic acidaemia is one of the rarest metabolic disorders reported in both early neonatal life and the childhood period. Understanding the clinical presentation and MRI findings with specific DWI changes reported in this case assists the health care provider in making the appropriate clinical management and metabolic screening decisions. This case with these rare and specific DWI changes seems to be unique to the propionic acidaemia crisis which reinforces MRI findings and changes mentioned previously in the literatures.
A practical primer for image-based particle measurements in microplastic research
Authors: Schnepf Uwe , von Moers-Meßmer Maria Anna Lioba , Brümmer Franz 
Published: July 2023

AbstractMicroplastics have been detected in large numbers around the world. Not only their sheer number threatens ecosystems, their biodiversity, and human health, but risks are also posed by particle characteristics such as size and shape. However, at the moment their measurement is neither comprehensive nor harmonized, making the data ineligible for risk assessment. To change this, we propose an image-based workflow, whose six steps are oriented to international guidelines and lessons learned from more developed research fields. Best practices for sample preparation, image acquisition, and digital image processing are reviewed to assure accurate and unbiased particle measurements. On behalf of this, we selected metrics to quantitatively characterize both size and shape. The size of microplastics should be estimated via the maximum Feret’s diameter. Particle shape can be measured via shape descriptors, for which we derive harmonized formulas and interpretation.Roundness,solidity, andelongationwere selected by applying hierarchical agglomerative clustering and correlation analysis. With these three shape descriptors, all currently charaterizable dimensions of particle shape can be measured. Finally, we present actions for quality control as well as quality assurance and give recommendations for method documentation and data reporting. By applying our practical primer, microplastic researchers should be capable of providing informative and comparable data on particle characteristics. From this improved data, we expect to see great progress in risk assessment, meta-analyses, theory testing, and fate modeling of microplastics.