bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis, structural and electrochemical properties of V4O9 cathode for lithium batteries
Authors: Senguttuvan Premkumar , Lee Eungje , Key Baris , Johnson Christopher S. 
Published: April 2023

Single-phase three-dimensional vanadium oxide (V4O9) was synthesized by reduction of V2O5 using a gas stream of ammonia/argon (NH3/Ar). The as-synthesized oxide, prepared by this simple gas reduction method was subsequently electrochemically transformed into a disordered rock salt type-“Li3.7V4O9” phase while cycling over the voltage window 3.5 to 1.8 V versus Li. The Li-deficient phase delivers an initial reversible capacity of ∼260 mAhg−1 at an average voltage of 2.5 V vs. Li+/Li0. Further cycling to 50 cycles yields a steady 225 mAhg−1. Ex situ X-ray diffraction studies confirmed that (de) intercalation phenomena follows a solid-solution electrochemical reaction mechanism. As demonstrated, the reversibility and capacity utilization of this V4O9 is found to be superior to battery grade, micron-sized V2O5 cathodes in lithium cells.
Multimodal region-based behavioral modeling for suicide risk screening
Authors: Alghowinem Sharifa , Zhang Xiajie , Breazeal Cynthia , Park Hae Won 
Published: April 2023

IntroductionSuicide is a leading cause of death around the world, interpolating a huge suffering to the families and communities of the individuals. Such pain and suffering are preventable with early screening and monitoring. However, current suicide risk identification relies on self-disclosure and/or the clinician's judgment.Research question/statmentTherefore, we investigate acoustic and nonverbal behavioral markers that are associated with different levels of suicide risks through a multimodal approach for suicide risk detection.Given the differences in the behavioral dynamics between subregions of facial expressions and body gestures in terms of timespans, we propose a novel region-based multimodal fusion.MethodsWe used a newly collected video interview dataset of young Japanese who are at risk of suicide to extract engineered features and deep representations from the speech, regions of the face (i.e., eyes, nose, mouth), regions of the body (i.e., shoulders, arms, legs), as well as the overall combined regions of face and body.ResultsThe results confirmed that behavioral dynamics differs between regions, where some regions benefit from a shorter timespans, while other regions benefit from longer ones. Therefore, a region-based multimodal approach is more informative in terms of behavioral markers and accounts for both subtle and strong behaviors. Our region-based multimodal results outperformed the single modality, reaching a sample-level accuracy of 96% compared with the highest single modality that reached sample-level accuracy of 80%. Interpretation of the behavioral markers, showed the higher the suicide risk levels, the lower the expressivity, movement and energy observed from the subject. Moreover, the high-risk suicide group express more disgust and contact avoidance, while the low-risk suicide group express self-soothing and anxiety behaviors.DiscussionEven though multimodal analysis is a powerful tool to enhance the model performance and its reliability, it is important to ensure through a careful selection that a strong behavioral modality (e.g., body movement) does not dominate another subtle modality (e.g., eye blink). Despite the small sample size, our unique dataset and the current results adds a new cultural dimension to the research on nonverbal markers of suicidal risks. Given a larger dataset, future work on this method can be useful in helping psychiatrists with the assessment of suicide risk and could have several applications to identify those at risk.
Mendelian randomization analysis does not reveal a causal influence of mental diseases on osteoporosis
Authors: Tang Fen , Wang Sheng , Zhao Hongxia , Xia Demeng , Dong Xin 
Published: April 2023

IntroductionOsteoporosis (OP) is primarily diagnosed through bone mineral density (BMD) measurements, and it often leads to fracture. Observational studies suggest that several mental diseases (MDs) may be linked to OP, but the causal direction of these associations remain unclear. This study aims to explore the potential causal association between five MDs (Schizophrenia, Depression, Alzheimer's disease, Parkinson's disease, and Epilepsy) and the risk of OP.MethodsFirst, single-nucleotide polymorphisms (SNPs) were filtered from summary-level genome-wide association studies using quality control measures. Subsequently, we employed two-sample Mendelian randomization (MR) analysis to indirectly analyze the causal effect of MDs on the risk of OP through bone mineral density (in total body, femoral neck, lumbar spine, forearm, and heel) and fractures (in leg, arm, heel, spine, and osteoporotic fractures). Lastly, the causal effect of the MDs on the risk of OP was evaluated directly through OP. MR analysis was performed using several methods, including inverse variance weighting (IVW)-random effects, IVW-fixed effects, maximum likelihood, weighted median, MR-Egger regression, and penalized weighted median.ResultsThe results did not show any evidence of a causal relationship between MDs and the risk of OP (with almost all P values &gt; 0.05). The robustness of the above results was proved to be good.DiscussionIn conclusion, this study did not find evidence supporting the claim that MDs have a definitive impact on the risk of OP, which contradicts many existing observational reports. Further studies are needed to determine the potential mechanisms of the associations observed in observational studies.
Application of circulating tumour DNA in terms of prognosis prediction in Chinese follicular lymphoma patients
Authors: Zhao Mengjing , Li Qingjuan , Yang Jing , Zhang Min , Liu Xiaolan , Zhang Hongwei , Huang Yunpeng , Li Jing , Bao Jiangping , Wang Jingfang , Du Jun , Guan Tao , Su Liping 
Published: April 2023

Background: Follicular lymphoma (FL), an indolent non-Hodgkin lymphoma (NHL), is generally incurable. Favourable prognosis and durable remission are crucial for FL patients. The genetic mutation spectrum provides novel biomarkers for determining the prognosis of FL patients, but its detection is easily affected by the collection of tumour tissue biopsies. In this study, we aimed to describe the mutational landscape of FL using circulating tumour DNA (ctDNA) samples and to explore the relationship between mutations and prognostic indicators of clinical outcome in patients with newly diagnosed follicular lymphoma and the prognostic value of such mutations.Methods: A total of 28 patients with newly diagnosed FL were included in this study. A targeted NGS-based 59-gene panel was used to assess the ctDNA mutation profiles. Differences in clinical factors between patients carrying mutations and those without mutations were analysed. We also explored the relationship between gene mutation status, mean VAFs (variant allele frequencies) and clinical factors. The Kaplan‒Meier method was applied to analyse the overall survival (OS) and progression-free survival (PFS) of patients carrying mutations and those without mutations.Results: ctDNA mutations were detectable in 21 (75%) patients. The most commonly mutated genes were CREBBP (54%, 15/28), KMT2D (50%, 14/28), STAT6 (29%, 8/28), CARD11 (18%, 5/28), PCLO (14%, 4/28), EP300 (14%, 4/28), BCL2 (11%, 3/28), and TNFAIP3 (11%, 3/28), with a mutation frequency of &gt;10%. Patients with detectable ctDNA mutation tended to present with advanced Ann Arbor stage (III-IV) (p = 0.009), high FLIPI risk (3–5) (p = 0.023) and severe lymph node involvement (No. of involved areas ≥5) (p = 0.02). In addition, we found that the mean VAF was significantly higher in patients with advanced Ann Arbor stage, high-risk FLIPI, elevated lactate dehydrogenase (LDH: 0–248U/L), advanced pathology grade, bone marrow involvement (BMI) and lymph node involvement. Additionally, KMT2D, EP300, and STAT6 mutations were associated with inferior PFS (p &lt; 0.05).Conclusion: We described the ctDNA mutation landscapes in Chinese patients with newly diagnosed FL and found that ctDNA VAF means reflect tumour burden. Moreover, PFS was shorter in patients with KMT2D, EP300 and STAT6 mutations.
Combining globally search for a regular expression and print matching lines with bibliographic monitoring of genomic database improves diagnosis
Authors: Tran Mau-Them Frédéric , Overs Alexis , Bruel Ange-Line , Duquet Romain , Thareau Mylene , Denommé-Pichon Anne-Sophie , Vitobello Antonio , Sorlin Arthur , Safraou Hana , Nambot Sophie , Delanne Julian , Moutton Sebastien , Racine Caroline , Engel Camille , De Giraud d’Agay Melchior , Lehalle Daphne , Goldenberg Alice , Willems Marjolaine , Coubes Christine , Genevieve David , Verloes Alain , Capri Yline , Perrin Laurence , Jacquemont Marie-Line , Lambert Laetitia , Lacaze Elodie , Thevenon Julien , Hana Nadine , Van-Gils Julien , Dubucs Charlotte , Bizaoui Varoona , Gerard-Blanluet Marion , Lespinasse James , Mercier Sandra , Guerrot Anne-Marie , Maystadt Isabelle , Tisserant Emilie , Faivre Laurence , Philippe Christophe , Duffourd Yannis , Thauvin-Robinet Christel 
Published: April 2023

Introduction: Exome sequencing has a diagnostic yield ranging from 25% to 70% in rare diseases and regularly implicates genes in novel disorders. Retrospective data reanalysis has demonstrated strong efficacy in improving diagnosis, but poses organizational difficulties for clinical laboratories.Patients and methods: We applied a reanalysis strategy based on intensive prospective bibliographic monitoring along with direct application of the GREP command-line tool (to “globally search for a regular expression and print matching lines”) in a large ES database. For 18 months, we submitted the same five keywords of interest [(intellectual disability, (neuro)developmental delay, and (neuro)developmental disorder)] to PubMed on a daily basis to identify recently published novel disease–gene associations or new phenotypes in genes already implicated in human pathology. We used the Linux GREP tool and an in-house script to collect all variants of these genes from our 5,459 exome database.Results: After GREP queries and variant filtration, we identified 128 genes of interest and collected 56 candidate variants from 53 individuals. We confirmed causal diagnosis for 19/128 genes (15%) in 21 individuals and identified variants of unknown significance for 19/128 genes (15%) in 23 individuals. Altogether, GREP queries for only 128 genes over a period of 18 months permitted a causal diagnosis to be established in 21/2875 undiagnosed affected probands (0.7%).Conclusion: The GREP query strategy is efficient and less tedious than complete periodic reanalysis. It is an interesting reanalysis strategy to improve diagnosis.
Identification of non-coding RNA related prognosis biomarkers based on ceRNA network in thyroid cancer
Authors: Fang Xin , Chen Xiliang , Gao Jingquan , Tong Liquan 
Published: April 2023

Introduction: Thyroid cancer (THCA) has become a serious malignant tumor worldwide. Identification of non-coding RNA related regulators is very necessary to improve the knowledge of THCA treatment. The aim of this study was to identify novel therapeutic targets and prognosis biomarkers for predicting pathological characteristics and subsequently treating THCA.Methods: We investigated the alterations of miRNAs, mRNAs and lncRNAs in THCA. Functional enrichment and clustering analysis were conducted for these aberrantly expressed RNAs. Multiple interaction networks among miRNAs, mRNAs and lncRNAs were constructed and the functional modules associated with THCA patients’ prognosis were identified. Furthermore, we evaluated the prognostic roles of the important miRNAs, mRNAs and lncRNAs in THCA and investigated the regulatory potential of non-coding RNAs on immune cell infiltration.Results: We firstly identified that miR-4709-3p and miR-146b-3p could significantly classify patients into high/low risk groups, which may be potential prognosis biomarkers of THCA. Secondly, we constructed a THCA-related miRNA-mRNA network, which displayed small world network topological characters. Two THCA-related functional modules were identified from the miRNA-mRNA network by MCODE. Results showed that two modules could implicate in known cancer pathways, such as apoptosis and focal adhesion. Thirdly, a THCA-related miRNA-lncRNA network was constructed. A subnetwork of miRNA-lncRNA network showed strong prognosis effect in THCA. Fourthly, we constructed a THCA-related mRNA-lncRNA network and detected several typical lncRNA-miRNA-mRNA crosstalk, such as AC068138, BCL2, miR-21 and miR-146b, which had good prognosis effect in THCA. Immune infiltration results showed that lncRNAs LA16c−329F2, RP11−395N3, RP11−423H2, RP11−399B17 and RP11–1036E20 were high related to neutrophil and dendritic cell infiltration.Discussion: Non-coding RNA-mediated gene regulatory network has the strong regulatory potential in pathological processes of THCA. All these results could help us uncover the non-coding RNA-mediated regulatory mechanism in THCA.
Copper and cuproptosis-related genes in hepatocellular carcinoma: therapeutic biomarkers targeting tumor immune microenvironment and immune checkpoints
Authors: Wang Xiaoqiang , Chen Dongfang , Shi Yumiao , Luo Jiamei , Zhang Yiqi , Yuan Xiaohong , Zhang Chaojin , Shu Huigang , Yu Weifeng , Tian Jie 
Published: April 2023

BackgroundHepatocellular carcinoma (HCC), one of the most common cancers worldwide, exhibits high immune heterogeneity and mortality. Emerging studies suggest that copper (Cu) plays a key role in cell survival. However, the relationship between Cu and tumor development remains unclear.MethodsWe investigated the effects of Cu and cuproptosis-related genes (CRGs) in patients with HCC in the TCGA-LIHC (The Cancer Genome Atlas-Liver cancer, n = 347) and ICGC-LIRI-JP (International Cancer Genome Consortium-Liver Cancer-Riken-Japan, n = 203) datasets. Prognostic genes were identified by survival analysis, and a least absolute shrinkage and selection operator (Lasso) regression model was constructed using the prognostic genes in the two datasets. Additionally, we analyzed differentially expressed genes and signal pathway enrichment. We also evaluated the effects of CRGs on tumor immune cell infiltration and their co-expression with immune checkpoint genes (ICGs) and performed validation in different tumor immune microenvironments (TIMs). Finally, we performed validation using clinical samples and predicted the prognosis of patients with HCC using a nomogram.ResultsA total of 59 CRGs were included for analysis, and 15 genes that significantly influenced the survival of patients in the two datasets were identified. Patients were grouped by risk scores, and pathway enrichment analysis suggested that immune-related pathways were substantially enriched in both datasets. Tumor immune cell infiltration analysis and clinical validation revealed that PRNP (Prion protein), SNCA (Synuclein alpha), and COX17 (Cytochrome c oxidase copper chaperone COX17) may be closely correlated with immune cell infiltration and ICG expression. A nomogram was constructed to predict the prognosis of patients with HCC using patients’ characteristics and risk scores.ConclusionCRGs may regulate the development of HCC by targeting the TIM and ICGs. CRGs such as PRNP, SNCA, and COX17 could be promising targets for HCC immune therapy in the future.
Effect of NETs/COX-2 pathway on immune microenvironment and metastasis in gastric cancer
Authors: Zhang Ange , Zou Xiaoming , Yang Shifeng , Yang Hao , Ma Zhen , Li Jiacheng 
Published: April 2023

BackgroundNeutrophil extracellular traps (NETs) are crucial in the progression of several cancers. The formation of NETs is closely related to reactive oxygen species (ROS), and the granule proteins involved in nucleosome depolymerization under the action of ROS together with the loosened DNA compose the basic structure of NETs. This study aims to investigate the specific mechanisms of NETs promoting gastric cancer metastasis in order to perfect the existing immunotherapy strategies.MethodsIn this study, the cells and tumor tissues of gastric cancer were detected by immunological experiments, real-time polymerase chain reaction and cytology experiments. Besides, bioinformatics analysis was used to analyze the correlation between cyclooxygenase-2 (COX-2) and the immune microenvironment of gastric cancer, as well as its effect on immunotherapy.ResultsExamination of clinical specimens showed that NETs were deposited in tumor tissues of patients with gastric cancer and their expression was significantly correlated with tumor staging. Bioinformatics analysis showed that COX-2 was involved in gastric cancer progression and was associated with immune cell infiltration as well as immunotherapy. In vitro experiments, we demonstrated that NETs could activate COX-2 through Toll-like receptor 2 (TLR2) and thus enhance the metastatic ability of gastric cancer cells. In addition, in a liver metastasis model of nude mice we also demonstrated the critical role of NETs and COX-2 in the distant metastasis of gastric cancer.ConclusionNETs can promote gastric cancer metastasis by initiating COX-2 through TLR2, and COX-2 may become a target for gastric cancer immunotherapy.
Clinical and histopathological responses to bee venom phonophoresis in treating venous and diabetic ulcers: a single-blind randomized controlled trial
Authors: Othman Eman M. , Hamada Hamada Ahmed , Mohamed Ghada I. , Abdallah Ghada A. , Ahmed Zeinab S. , Al-Shenqiti Abdullah M. , Kadry Ahmed Mahmoud 
Published: April 2023

IntroductionChronic venous and diabetic ulcers are hard to treat that cause patients long time of suffering as well as significant healthcare and financial costs.PurposeThe conducted study was to evaluate the efficacy of bee venom (BV) phonophoresis on the healing of chronic unhealed venous and/or diabetic foot ulcers Also, to compare the healing rate of diabetic and venous ulcers.MethodologyThe study included 100 patients (71 males and 29 females) with an age range of 40-60 years' old who had chronic unhealed venous leg ulcers of grade I, grade II, or diabetic foot ulcers with type II diabetes mellitus. They randomly assigned into four equal groups of 25: Group A (diabetic foot ulcer study group) and group C (venous ulcer study group) who both received conservative treatment of medical ulcer care and phonophoresis with BV gel, in addition to group B (diabetic foot ulcer control group) and group D (venous ulcer control group) who both received conservative treatment of medical ulcer care and received ultrasound sessions only without BV gel. Wound surface area (WSA) and ulcer volume measurement (UVM) were used to assess the ulcer healing pre-application (P0), post-6 weeks of treatment (P1), and after 12 weeks of treatment (P2). In addition to Ki-67 immunohistochemistry was used to evaluate the cell proliferative in the granulation tissue of ulcers pre-application (P0) and after 12 weeks of treatment (P2) for all groups.ResultsThis research revealed a statistical significance improvement (p ≤ 0.0) in the WSA, and UVM with no significant difference between study groups after treatment. Regarding Ki-67 immunohistochemistry showed higher post treatment values in the venous ulcer group in comparison to the diabetic foot ulcer group.ConclusionBee venom (BV) provided by phonophoresis is effective adjuvant treatment in accelerating venous and diabetic foot ulcer healing with higher proliferative effect on venous ulcer.Clinical trial registrationwww.ClinicalTrials.gov, identifier: NCT05285930.
25(OH)D-but not 1,25(OH)2D–Is an independent risk factor predicting graft loss in stable kidney transplant recipients
Authors: Zeng Shufei , Yang Yide , Li Shuping , Hocher Carl-Friedrich , Chu Chang , Wang Ziqiang , Zheng Zhihua , Krämer Bernhard K. , Hocher Berthold 
Published: April 2023

BackgroundVitamin D deficiency (VDD) or vitamin D insufficiency is common in kidney transplant recipients (KTRs). The impact of VDD on clinical outcomes in KTRs remain poorly defined and the most suitable marker for assessing vitamin D nutritional status in KTRs is unknown so far.MethodsWe conducted a prospective study including 600 stable KTRs (367 men, 233 women) and a meta-analysis to pool existing evidence to determine whether 25(OH)D or 1,25(OH)2D predicted graft failure and all-cause mortality in stable KTRs.ResultsCompared with a higher 25(OH)D concentration, a low concentration of 25(OH)D was a risk factor for graft failure (HR 0.946, 95% CI 0.912−0.981, p = 0.003), whereas 1,25 (OH)2D was not associated with the study end-point graft loss (HR 0.993, 95% CI 0.977−1.009, p = 0.402). No association was found between either 25(OH)D or 1,25 (OH)2D and all-cause mortality. We furthermore conducted a meta-analysis including 8 studies regarding the association between 25(OH)D or 1,25(OH)2D and graft failure or mortality, including our study. The meta-analysis results were consistent with our study in finding that lower 25(OH)D levels were significantly associated with the risk of graft failure (OR = 1.04, 95% CI: 1.01−1.07), but not associated with mortality (OR = 1.00, 95% CI: 0.98−1.03). Lower 1,25(OH)2D levels were not associated with the risk of graft failure (OR = 1.01, 95% CI: 0.99−1.02) and mortality (OR = 1.01, 95% CI: 0.99−1.02).ConclusionBaseline 25(OH)D concentrations but not 1,25(OH)2D concentrations were independently and inversely associated with graft loss in adult KTRs.
Determination of the effective dose of dexmedetomidine to achieve loss of consciousness during anesthesia induction
Authors: Mu Bing , Xu Wenjie , Li Hongyi , Suo Zizheng , Wang Xiaoxiao , Zheng Yuxiang , Tian Yi , Zhang Bowen , Yu Jie , Tian Naiyuan , Lin Nan , Zhao Dan , Zheng Zhaoxu , Zheng Hui , Ni Cheng 
Published: April 2023

BackgroundDexmedetomidine (DEX) is a sedative with greater preservation of cognitive function, reduced respiratory depression, and improved patient arousability. This study was designed to investigate the performance of DEX during anesthesia induction and to establish an effective DEX induction strategy, which could be valuable for multiple clinical conditions.MethodsPatients undergoing abdominal surgery were involved in this dose-finding trial. Dixon's up-and-down sequential method was employed to determine the effective dose of DEX to achieve the state of “loss of consciousness”, and an effective induction strategy was established with continuous infusion of DEX and remifentanil. The effects of DEX on hemodynamics, respiratory state, EEG, and anesthetic depth were monitored and analyzed.ResultsThrough the strategy mentioned, the depth of surgical anesthesia was successfully achieved by DEX-led anesthesia induction. The ED50 and ED95 of the initial infusion rate of DEX were 0.115 and 0.200 μg/kg/min, respectively, and the mean induction time was 18.3 min. The ED50 and ED95 of DEX to achieve the state of “loss of consciousness” were 2.899 (95% CI: 2.703–3.115) and 5.001 (95% CI: 4.544–5.700) μg/kg, respectively. The mean PSI on the loss of consciousness was 42.8 among the patients. During anesthesia induction, the hemodynamics including BP and HR were stable, and the EEG monitor showed decreased α and β powers and increased θ and δ in the frontal and pre-frontal cortices of the brain.ConclusionThis study indicated that continuous infusion of combined DEX and remifentanil could be an effective strategy for anesthesia induction. The EEG during the induction was similar to the physiological sleep process.