bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of Kmt2c or Kmt2d primes urothelium for tumorigenesis and redistributes KMT2A–menin to bivalent promoters
Authors: Wang Naitao , Pachai Mohini R. , Li Dan , Lee Cindy J. , Warda Sarah , Khudoynazarova Makhzuna N. , Cho Woo Hyun , Xie Guojia , Shah Sagar R. , Yao Li , Qian Cheng , Wong Elissa W. P. , Yan Juan , Tomas Fanny V. , Hu Wenhuo , Kuo Fengshen , Gao Sizhi P. , Luo Jiaqian , Smith Alison E. , Han Ming , Gao Dong , Ge Kai , Yu Haiyuan , Chandarlapaty Sarat , Iyer Gopakumar V. , Rosenberg Jonathan E. , Solit David B. , Al-Ahmadie Hikmat A. , Chi Ping , Chen Yu 
Published: January 2025

Abstract
Members of the KMT2C/D–KDM6A complex are recurrently mutated in urothelial carcinoma and in histologically normal urothelium. Here, using genetically engineered mouse models, we demonstrate that Kmt2c/d knockout in the urothelium led to impaired differentiation, augmented responses to growth and inflammatory stimuli and sensitization to oncogenic transformation by carcinogen and oncogenes. Mechanistically, KMT2D localized to active enhancers and CpG-poor promoters that preferentially regulate the urothelial lineage program and Kmt2c/d knockout led to diminished H3K4me1, H3K27ac and nascent RNA transcription at these sites, which leads to impaired differentiation. Kmt2c/d knockout further led to KMT2A–menin redistribution from KMT2D localized enhancers to CpG-high and bivalent promoters, resulting in derepression of signal-induced immediate early genes. Therapeutically, Kmt2c
/
d knockout upregulated epidermal growth factor receptor signaling and conferred vulnerability to epidermal growth factor receptor inhibitors. Together, our data posit that functional loss of Kmt2c/d licenses a molecular ‘field effect’ priming histologically normal urothelium for oncogenic transformation and presents therapeutic vulnerabilities.
Evaluation of osseointegration of plasma treated polyaryletherketone maxillofacial implants
Authors: Al Maruf D S Abdullah , Ren Jiongyu , Cheng Kai , Xin Hai , Lewin Will , Pickering Edmund , Kruse Hedi Verena , Leinkram David , Parthasarathi Krishnan , Wise Innes , Filippi Benjamin , Beirne Stephen , Froggatt Cate , Wykes James , Howes Dale , Suchowerska Natalka , Woodruff Maria A. , Crook Jeremy M. , McKenzie David R. , Clark Jonathan R. 
Published: January 2025

Abstract
Osseointegration is a crucial property of biomaterials used for bone defect repair. While titanium is the gold standard in craniofacial surgeries, various polymeric biomaterials are being explored as alternatives. However, polymeric materials can be bioinert, hindering integration with surrounding tissues. In this investigation, plasma ion immersion implantation (PIII)-treated polyether ether ketone (PEEK) and polyether ketone (PEK) implants were assessed in a sheep maxilla and mandible model. Defects were filled with PIII-treated PEEK and PEK implants, produced through fused filament fabrication (FFF) and selective laser sintering (SLS), respectively. Positive controls were grade 23 titanium implants via selective laser melting, while untreated PEEK implants served as negative controls. Surface analyses using scanning electron microscopy and atomic force microscopy revealed favorable properties. Osseointegration was qualitatively and quantitatively assessed at 8-, 10-, and 12-weeks post-implantation, showing significantly improved outcomes for both PIII-treated PEEK and PEK implants compared to untreated controls. The study suggests PIII treatment enhances FFF-printed PEEK’s osseointegration, and PIII-treated SLS-printed PEK achieves comparable osseointegration to 3D printed titanium. These findings underscore surface modification strategies’ potential for polymeric biomaterials, offering insights into developing alternative implant materials for craniofacial surgeries, with enhanced biocompatibility and osseointegration capabilities for improved clinical outcomes.
Therapeutic potential of brentuximab vedotin in breast cancer and lymphoma via targeted apoptosis and gene regulation
Authors: Ezzat Abeer , Shafiek Mohga , Shawki Shimaa , Abdel-Ghany Shaimaa , Nazih Mahmoud , Sabit Hussein 
Published: January 2025

Abstract
This study was designed to assess the effect of brentuximab vedotin on several breast cancer cell lines in terms of promoting apoptosis and managing cancer progression. Additionally, the study investigated the potential of repurposing this drug for new therapeutic reasons, beyond its original indications. The study evaluates the cytotoxic effects of Brentuximab vedotin across five cell lines: normal human skin fibroblasts (HSF), three breast cancer cell lines (MCF-7, MDA-MB-231, and T-47D), and histiocytic lymphoma (U-937). Brentuximab treatment was administered at four time points (0, 24, 48, and 72 h), with cell viability assessed at each interval. HSF cells, serving as controls, exhibited minimal viability loss (above 70%), indicating limited toxicity in normal fibroblasts. In contrast, MCF-7 and MDA-MB-231 cells demonstrated time-dependent reductions in viability, with a pronounced decline by 72 h, suggesting Brentuximab’s efficacy in both ER-positive and triple-negative breast cancer. T-47D cells also showed decreased viability, though at a slower rate. U-937 cells exhibited the most substantial reduction, highlighting Brentuximab’s potent activity against hematologic malignancies. Wound healing assays further revealed that Brentuximab significantly impaired the migration and healing capacity of cancer cells compared to untreated controls. Additionally, cell cycle analysis indicated G2/M phase arrest in cancer cells, particularly in MCF-7 and MDA-MB-231, while HSF cells remained largely unaffected. Apoptosis detection confirmed Brentuximab-induced cell death, with significant increases in late apoptosis in cancer lines, especially by 72 h. Gene expression analysis revealed upregulation of pro-apoptotic genes (BAX, Caspase 3, and Caspase 9) in cancer cells, alongside a decrease in anti-apoptotic BCL-2 expression. These findings suggest Brentuximab’s selective cytotoxicity against cancer cells and its potential as an effective therapeutic agent, particularly in breast cancer and histiocytic lymphoma.
The global, regional, and national patterns of change in the burden of bacterial pyoderma from 1990 to 2019 and the forecast for the next decade
Authors: Wang Hao , Bai Zihao , Shen Chong , Kou Jiaxi , Zhu Yanqing , Xie Huaxia , Chen Chen , Mo Ran 
Published: January 2025

Abstract
Pyoderma, commonly known as impetigo, is a bacterial skin infection causing pus formation, prevalent globally, especially in resource-poor areas. It affects both children and adults, including those with conditions like diabetes. Despite its significant impact and economic burden, research on its global epidemiology is limited. This study aims to address this gap by analyzing pyoderma trends from 1990 to 2019 using GBD data. The study aims to analyze global trends in pyoderma epidemiology from 1990 to 2019 using GBD data. Specifically, it investigates Age-Standardized Incidence Rate (ASIR), Mortality Rate (ASMR), and Disability-Adjusted Life Years Rate (ASDR) across 204 countries. Additionally, it provides insights into demographic and socioeconomic factors influencing pyoderma prevalence. Furthermore, it forecasts pyoderma’s development trends for the next decade to inform public health strategies. Data were sourced from the GBD 2019 database, comprising various sources such as censuses, surveys, and registries. Estimates for pyoderma incidence, mortality, and DALYs, along with their 95% uncertainty intervals (UI), were retrieved. The Sociodemographic Index (SDI) was used to assess socioeconomic status, and statistical calculations were performed using the WHO Health Equity Assessment Toolkit and R software (v4.3.2). From 1990 to 2019, the Age-Standardized Incidence Rate (ASIR) and Age-Standardized Mortality Rate (ASMR) of impetigo increased, while the Age-Standardized Disability-Adjusted Life Years Rate (ASDR) declined. Significant global geographical heterogeneity persists, closely associated with the Sociodemographic Index (SDI). Children under 5 and the elderly are particularly at risk, with lower SDI nations bearing higher burdens. Population growth and aging contribute to this rise, with disparities in impetigo trends persisting among countries and regions with varying SDI levels, expected to continue until 2030. Pyoderma exhibits significant heterogeneity across age, gender, and geography, with pronounced disparities evident in underdeveloped regions or countries. Therefore, prioritizing policy formulation and implementing tailored prevention and treatment strategies for high-risk populations are imperative to alleviate the disease burden effectively. Such targeted approaches are crucial in addressing the global impact of pyoderma.
Sinomenine attenuates uremia vascular calcification by miR-143-5p
Authors: Yu Fengyi , Peng Zhong , Gao Ning , Tang Zixu , Liao Zihao , Zhao Song , Zhong Shuzhu , Umwiza Gloria , Huang Hong , Long Wei , He Zhangxiu 
Published: January 2025

Abstract
Vascular calcification is considered to be a killer of the cardiovascular system, involved inflammation and immunity. There is no approved therapeutic strategy for the prevention of vascular calcification. Sinomenine exhibited anti-inflammatory and immunosuppressive effects. Objective of this study was to investigate the effect of sinomenine in vascular calcification and its potential molecular mechanism. Adenine-induced uremic rats were constructed and administrated with sinomenine. Optical clearing of aortas, alizarin red staining, von Kossa staining, calcification quantification, micro-CT analyses of vascular calcification were performed to analyze calcification in aortas. Administration of 40 mg/kg/d sinomenine effectively alleviated vascular calcification in uremic rats. The miRNA sequencing revealed differentially expressed miRNAs in aortas and bioinformatic analysis assisted with miRNA screening. We screened 9 differential expressed miRNAs and their predicted target genes. By qRT-PCR, we validated that the expression of rno-miR-143-5p was corresponding to our prediction. Sinomenine inhibited vascular smooth muscle cells (VSMCs) calcification, accompanied with miR-143-5p upregulation. MiR-143-5p mimic decreased VSMCs calcification in high phosphate condition. On the contrary, miR-143-5p inhibitor increased VSMCs calcification in high phosphate condition, which was inhibited by sinomenine. In chronic kidney disease patients with vascular calcification, the expression level of circulating miR-143-5p was lower than those without vascular calcification. Sinomenine significantly inhibited vascular calcification in VSMCs and uremic rat. MiR-143-5p was one of the collection of miRNAs modified by sinomenine in vascular calcification. Reduction of miR-143-5p in VSMCs was not only a concomitant phenomenon in pro-calcification condition but also contribute to VSMCs calcification. Circulating miR-143-5p was supposed to be a potential biomarker for vascular calcification in chronic kidney disease patients. In conclusion, sinomenine effectively alleviated vascular calcification, which was attributed to miR-143-5p regulation partly.
Confined and mined: anthropogenic river modification as a driver of flood risk change
Authors: Quick Laura , Williams R. D. , Boothroyd R. J. , Hoey T. B. , Tolentino P. L. M. , MacDonell C. , Guardian E. , Reyes J. , Sabillo C. , Perez J. , David C. P. C. 
Published: January 2025

Abstract
Rivers are often confined by structures and subjected to aggregate mining. In dynamic rivers, these interventions cause changes to riverbed and bank topography that potentially cause changes in hydraulics and flood risk. Repeat, system-scale, high-resolution topographic surveys of the gravel-bed Bislak River, the Philippines, are used to quantify annual morphological change and, using two-dimensional hydraulic modelling, to simulate changes to flood risk. Aggregate mining exports sediment and creates pitted topography, and embankments cause both deeper channels and disconnect the river from its floodplain. The consequently increased channel capacity reduces flood risk, with up to a 5% decrease in inundated areas for 10- to 100-year return periods. Sediment deprivation also increases bed shear stress that can induce scour, infrastructure damage and increased flood impacts. Rising global floodplain populations and increasing demand for aggregate ensure that sustainably managing geomorphologically dynamic rivers to support floodplain development and mitigate flood impacts remains a pertinent challenge.
Specialized pericyte subtypes in the pulmonary capillaries
Authors: Klouda Timothy , Kim Yunhye , Baek Seung-Han , Bhaumik Mantu , Li Yan , Liu Yu , Wu Joseph C , Raby Benjamin A , Perez Vinicio de Jesus , Yuan Ke 
Published: January 2025

Abstract
Pericytes are essential for capillary stability and homeostasis, with impaired pericyte function linked to diseases like pulmonary arterial hypertension. Investigating pericyte biology has been challenging due to the lack of specific markers, making it difficult to distinguish pericytes from other stromal cells. Using bioinformatic analysis and RNAscope, we identified Higd1b as a unique gene marker for pericytes and subsequently generated a knock-in mouse line, Higd1b-CreERT2, that accurately labels pericytes in the lung and heart. Single-cell RNA sequencing revealed two distinct Higd1b+ pericyte subtypes: while Type 1 pericytes support capillary homeostasis, Type 2 pericytes accumulate in arterioles, and co-express smooth muscle markers and higher levels of vimentin under hypoxic conditions. Lastly, healthy human lung pericytes with upregulation of vimentin exhibited increased adhesion, migration, and higher expression levels of the smooth muscle marker SM22 in vitro. These findings highlight the specialization of pulmonary pericytes and their contribution to vascular remodeling during hypoxia-induced pulmonary hypertension.
Breaking binary in cardiovascular disease risk prediction
Authors: Zhang Yichi , Fahed Akl C. 
Published: January 2025

Abstract
Atherosclerotic cardiovascular disease (ASCVD) remains the leading cause of death in the world. However, advances in genetics, omics research, machine learning (ML), and precision medicine have inspired revolutionary new tools in ASCVD risk stratification. Together, polygenic risk scores (PRS) and composite ML-based algorithms help shift the paradigm away from binary predictions towards more comprehensive continuum models. Continued efforts are needed to address socioeconomic and racial disparities in the PRS space.
Application of Artificial Intelligence In Drug-target Interactions Prediction: A Review
Authors: Liao Qian , Zhang Yu , Chu Ying , Ding Yi , Liu Zhen , Zhao Xianyi , Wang Yizheng , Wan Jie , Ding Yijie , Tiwari Prayag , Zou Quan , Han Ke 
Published: January 2025

Abstract
Predicting drug-target interactions (DTI) is a complex task. With the introduction of artificial intelligence (AI) methods such as machine learning and deep learning, AI-based DTI prediction can significantly enhance speed, reduce costs, and screen potential drug design options before conducting actual experiments. However, the application of AI methods also faces several challenges that need to be addressed. This article reviews various AI-based approaches and suggests possible future directions.
A deep learning‐based method for detecting and identifying surface defects in polyimide foam
Authors: Song Xianhui , Hu Guangzhong , Lu Jing , Tuo Xianguo , Li Yuedong 
Published: January 2025

AbstractCurrently, the detection and identification of surface defects in polyimide foam products mainly rely on on‐site work experience, which has issues such as low detection accuracy, strong subjectivity, and low efficiency. Existing research on foam product defect detection primarily targets internal defects, lacking studies on the detection, identification, and classification of surface defects. Therefore, this article proposes a method for identifying and classifying surface defects in polyimide foam based on an improved GoogLeNet, aiming to quickly and accurately detect and identify surface defects in foam products. By optimizing the Inception blocks, introducing the ECA attention mechanism, and adding an LSTM network module, the model's recognition accuracy and generalization ability are effectively improved. In experiments, the model proposed in this article performed excellently on the foam surface defect dataset, showing a significant advantage in detection accuracy compared to other convolutional neural network models. The detection accuracy for pits and cracks reached 98.24% and 98.25%, respectively, providing a reliable reference for the detection of surface defects in industrial foam production.
Design prognostics for 4400 TEU container vessel by multi‐variate Gaidai reliability approach
Authors: Zhu Yan , Gaidai Oleg , Sheng Jinlu , Ashraf Alia , Cao Yu , Liu Zirui 
Published: January 2025

AbstractThis case study introduces an innovative multivariate methodology for assessing the lifetime of marine engineering systems, specifically in cargo vessel transportation. The analysis focused on stress data collected onboard a 4400 TEU container vessel during multiple trans‐Atlantic voyages. One of the major challenges in marine cargo transport lies in mitigating the risk of container loss due to excessive whipping loads. Accurate prediction of extreme stress levels on vessel deck panels remains difficult, primarily because of the nonlinear and non‐stationary nature of wave and ship motion interactions. Higher‐order dynamic effects, such as second‐ and third‐order responses, often become significant when ships operate under adverse environmental conditions, amplifying nonlinear influences. Laboratory simulations, constrained by wave characteristics and scale similarity issues, may not always provide reliable results. Consequently, data collected from vessels navigating extreme weather conditions serves as a critical resource for comprehensive container ship risk assessment. The primary goal of this study was to validate and demonstrate the effectiveness of a novel multivariate risk evaluation approach, leveraging onboard measurements of dynamic areal pressure on cargo ship deck panels as the core dataset. The Gaidai methodology for multivariate risk evaluation proved to be a robust tool for assessing failure, hazard, and damage risks in complex, nonlinear vessel deck panel and ship hull stress systems.
Discovery and characterization of ZTF J0112+5827: An 80.9-minute polar with strong cyclotron features
Authors: Lin Jiamao , Ren Liangliang , Li Chengyuan , Nancy Elias-Rosa , Cang Tianqi , Ge Hongwei , Thomas Tam Pak-Hin , Huang Wenjun , Li Yilong , Wang Xiaofeng , Huang Yang , Ma Bo 
Published: January 2025

Context. A new X-ray Cataclysmic variable (CV) candidate exhibits distinct light-curve characteristics in the ZTF’s g, r, and i bands. The paper includes the optical identification and multiwavelength analysis of this CV candidate.
Aims. This work aims to determine if a previously identified CV candidate, ZTF J0112+5827, is a polar system by examining its X-ray and cyclotron radiation characteristics.
Methods. We characterized the X-ray emission of ZTF J0112+5827 using the ROSAT observations. The gri-band optical light curves were obtained from the Zwicky Transient Facility. After two nights of time-domain spectroscopic observations with the Palomar 200-inch telescope, we mapped the accretion structures using Doppler tomography.
Results. ZTF J0112+5827 exhibits an orbital period of 80.9 minutes, determined from the ZTF light curves, and an average X-ray flux of (68.4 ± 15.7)×10−14 erg s−1 cm−2 in the 0.1–2.4 keV range. It shows an ellipsoidal-like variability curve in the g band, with two prominent humps around phases of ∼0.0 and ∼0.7 in the i and r bands. In the spectra corresponding to these phases, a redward-increasing power-law continuum appears, which is accompanied by prominent features of cyclotron emission humps. Emission lines of He II and Balmer series were observed. The magnetic field strength of ZTF J0112+5827 was determined from the cyclotron harmonics. Its tomography map revealed the presence of accretion streams, but there was no evidence of an accretion disk structure. The line-of-sight velocity of the Balmer emission was measured at about 500 km s−1, the majority of which was contributed by accretion streams and accretion spots. Our result confirms that ZTF J0112+5827 is a polar system. It contains a magnetic white dwarf with a magnetic field strength of 38.7−1.1+1.3 MG.
Functional alignment is a feasible alignment strategy in robotic assisted total knee arthroplasty for knee osteoarthritis with extra-articular deformity – A case series
Authors: Eu Wei Cheong , Yuik Ho Jade Pei , Kunalan G. 
Published: January 2025

Introduction: Extraarticular deformity (EAD) with knee arthritis is a complex condition involving tri-planar bone deformity with pathological malalignment and chronic soft tissue contracture or laxity in the knee joint. Intraarticular correction by TKA, which was previously performed with conventional manual jig by mechanical alignment technique, had its limits and difficulties especially extensive soft tissue release and risk of jeopardizing the collateral ligaments. Robotic technology allows for reproducible and precise execution of surgical plan and allows adjustment to various new personalised alignment philosophy including functional alignment (FA). FA technique involves the adjustment of components positioning that least compromise the soft tissue envelope while restoring the limb alignment and joint obliquity to create a balanced knee. The aim is to study the outcome of intra-articular correction by robotic assisted TKA using Functional Alignment (FA) technique. Methodology: This is a single surgeon series of 8 patients with extraarticular deformity who underwent robotic assisted total knee arthroplasty (TKA) with FA technique. Soft tissue release was gradually released and followed by adjustments of implant positioning in order to achieve a balanced medio-lateral gap. Results: Postoperatively, the lower limb alignment of all patients were restored within 6° (mean 4.54°) based on functional alignment boundaries. Knee phenotype and joint line obliquity (JLO) were restored in comparison to contralateral lower limb. There were 6 varus and 2 valgus malalignment. 7 patients were implanted with posterior stabilized implants while 1 was implanted with cruciate retaining implant. Arc of knee flexion and extension improved (P = 0.002). There was a large postoperative improvement in the Knee Society Score (KSS) (P < 0.001). Discussion: Intraarticular correction by TKA for EAD with knee arthritis is technically reliable with robotic technology. It allows intraoperative adjustment following functional alignment philosophy, thereby, restoring pre-arthritic alignment, knee phenotype and joint line obliquity.