bluuebunny/crossref_metadata_2025_split huggingface dataset:
Substance use disorder treatment and technology access among people who use drugs in rural areas of the United States: A cross‐sectional survey
Authors: Button Dana , Levander Ximena A. , Cook Ryan R. , Miller William C. , Salisbury‐Afshar Elizabeth M. , Tsui Judith I. , Ibragimov Umedjon , Jenkins Wiley D. , Westergaard Ryan P. , Korthuis P. Todd 
Published: December 2022

AbstractPurposeTo evaluate how technology access affected substance use disorder (SUD) treatment prior to COVID‐19 for people who use drugs in rural areas.MethodsThe Rural Opioid Initiative (January 2018‐March 2020) was a cross‐sectional study of people with prior 30‐day injection drug or nonprescribed opioid use from rural areas of 10 states. Using multivariable mixed‐effect regression models, we examined associations between participant technology access and SUD treatment.FindingsOf 3,026 participants, 71% used heroin and 76% used methamphetamine. Thirty‐five percent had no cell phone and 10% had no prior 30‐day internet use. Having both a cell phone and the internet was associated with increased days of medication for opioid use disorder (MOUD) use (aIRR 1.29 [95% CI 1.11‐1.52]) and a higher likelihood of SUD counseling in the prior 30 days (aOR 1.28 [95% CI 1.05‐1.57]). Lack of cell phone was associated with decreased days of MOUD (aIRR 0.77 [95% CI 0.66‐0.91]) and a lower likelihood of prior 30‐day SUD counseling (aOR 0.77 [95% CI 0.62‐0.94]).ConclusionsExpanding US rural SUD treatment engagement via telemedicine may require increased cell phone and mobile network access.
Anxiety and depression in patients with narcolepsy
Authors: Abenza‐Abildua María José , Suárez‐Gisbert Eugenio , Lores‐Gutiérrez Vanesa , Algarra‐Lucas Carmen , Gómez‐Aceña Ángeles , Navacerrada‐Barrero Francisco José , González‐Martín Laura , Pérez‐Villena Ana , Pérez‐López Carlos 
Published: December 2022

SummaryWe analysed the co‐existence of psychopathology in patients with narcolepsy at our centre. We performed an observational retrospective descriptive analysis of patients with a diagnosis of narcolepsy, with and without psychopathology, who attended our sleep disorders unit from October 2012 to October 2021. A total of 51patients with narcolepsy (mean [SD] age 41.10 [14.71] years; 23 [45.1%] males and 28 [54.90%] females) were included. In all, 27 patients (52.94%) and 24 patients (47.06%) had narcolepsy with and without cataplexy, respectively. Of the total, 18 (33.33%) had a mood disorder: 18 with anxiety disorder (33.33%). Of these patients 14 (27.45%) had major depression, two (4%) had attempted suicide, one (2%) had manic outbreak, and one (2%) had substance abuse. Of the 18 patients with anxiety and depression, 10 (55.55%) and eight (44.44%) had narcolepsy with and without cataplexy, respectively. In the comparative analysis, a statistically significant relationship was found between younger age and the presence of anxiety. The prevalence of anxiety and depression in patients with narcolepsy was triple that of the general population, especially in younger patients. Psychopathology precedes the diagnosis of narcolepsy in most patients, not being reactive to diagnosis. This high prevalence suggests a possible biological relationship between both disorders, which should be assessed with larger studies.
Comparison of age estimates and population characteristics for green sunfish using two otolith methods
Authors: Griffin Austin D. , Porta Michael J. , Snow Richard A. 
Published: December 2022

AbstractDependent on the specific fishery, green sunfish (Lepomis cyanellus) can be viewed as a benefit or a detriment. Regardless, effective management of this species relies on precise age estimation, which is critical to detail age structure, growth and recruitment rates of their populations. To determine the most precise method for age estimation, estimated ages of whole and cracked otoliths of 422 green sunfish were compared from three small impoundments (0.6–16.2 ha) in Oklahoma. Green sunfish ages ranged from 0 to 7 for cracked otoliths and 0 to 6 for whole‐viewed otoliths, depending on the population. High precision between readers was observed for whole (per cent agreement = 85%–96%; mean CV = 3.4%–4.8%; and average per cent error = 2.4%–3.4%) and cracked otoliths (per cent agreement = 92%–96%; mean CV = 0.3%–6.3%; and average per cent error = 0.3%–4.4%). Limited bias was observed for young fish (≤age 3) for evaluating between‐reader precision using whole otoliths. When bias was present, ages generally exceeded the 1:1 line of equivalency. Final consensus ages for older fish (≥age 5) were sometimes underestimated using whole otoliths, compared to cracked otoliths. Fair agreement for final consensus ages (73%–87%) between whole and cracked otoliths was observed, compared to other otolith ageing studies in the literature. However, the results of the present study indicated no significant differences in growth or age‐frequency estimates using ages derived from whole and cracked otoliths, suggesting the moderate level of consensus agreement was not sufficiently low to significantly impact population characteristics and subsequent management decisions. Green sunfish growth rates in Oklahoma appear sufficiently high to allow the use of whole otoliths for age estimation to age seven, unless slow growth is expected. To ensure precise age estimation, however, it is suggested that otoliths from green sunfish ≥age 5 be cracked, regardless of growth rates.
Exploring the clinical features and risk factors for children tinea capitis complicated with allergic diseases
Authors: Zhou Xin , Liu Wentao , Yang Sulian , Wu Angela , Chen Peiran , Yin Songchao , de Hoog Sybren , Li Meirong , Feng Peiying 
Published: December 2022

AbstractBackgroundTinea capitis, atopic dermatitis and allergic rhinitis are the most common disorders endured by prepubescent children. Dermatophyte infections have been linked to allergic disorders, such as increased sensitivity to dermatophytes in patients with atopic dermatitis.ObjectivesTo explore the correlation between tinea capitis and allergic diseases in children and to analyse their risk factors.MethodsThis study monitored epidemiological changes in childhood tinea capitis and risk factors for whom with allergic disease in a single centre in three consecutive five‐year intervals by reviewing clinical data and multivariate logistic data analysis.ResultsBetween 2007 and 2022, there were 127 children patients with tinea capitis, the mean age was 4.83 years, and the male‐to‐female ratio was 1.76:1. Zoophilic Microsporum canis and Trichophyton mentagrophytes were the most prevalent pathogens, and the proportions remained relatively constant every 5 years. There were 34 (26.8%) children with tinea capitis complicated with allergic disease, among them 14 children with atopic dermatitis/eczema, 13 with allergic rhinitis, 8 urticaria, 6 food allergies and 1 allergic asthma. Male, kerion, zoophilic species infections and animal contact history were prevalent features in allergic disease combined with tinea capitis. Patients with tinea capitis plus allergic disease mostly had a family history with similar complications.ConclusionM. canis and T. mentagrophytes were the most prevalent pathogens of tinea capitis in the last 15 years; atopic dermatitis/eczema and allergic rhinitis were the most frequently associated allergic diseases. Male, kerion, zoophilic pathogen and animal contact history are risk factors.
Updated estimation of the burden of fungal disease in Vietnam
Authors: Duong Tra‐My N. , Le Minh‐Hang , Beardsley Justin , Denning David W. , Le Ngoc‐Huy , Nguyen Bich‐Ngoc T. 
Published: December 2022

AbstractBackgroundAnecdotally, the burden of fungal diseases in Vietnam is rapidly rising, but there has been no updated estimate on this issue since a previous report in 2015.ObjectivesIn this study, we aimed at estimating the incidence and prevalence of serious fungal infections for the year 2020.MethodsWe made estimates with a previously described methodology, using reports on the incidence and prevalence of various established risk factors for fungal infections from local, regional or global sources.ResultsWe estimated 2,389,661 cases of serious fungal infection occurred in Vietnam in 2020. The most common condition was recurrent vaginal candidiasis (4047/100,000 women annually). Among people living with HIV, we estimated 451 cases of cryptococcal meningitis, 1030 of pneumocystis pneumonia, 166 of histoplasmosis and 1612 of talaromycosis annually. Candidaemia incidence was estimated at 12/100,000 population each year. Owing to its high burden of tuberculosis and respiratory diseases, Vietnam had high rates of severe infections caused by Aspergillus species. Incidence of invasive aspergillosis is 24/100,000 population, allergic bronchopulmonary aspergillosis 78/100,000 and severe asthma with fungal sensitisation 102/100,000. Five‐year period prevalence of chronic pulmonary aspergillosis is 120/100,000 population /5‐year period. Mucormycosis, fungal keratitis and tinea capitis were estimated at 192, 14,431 and 201 episodes each year, respectively.ConclusionsThe number of patients with mycoses in Vietnam is likely underestimated due to a lack of local data and limited diagnostic capacity, but at least 2.5% of the population might have some form of serious fungal disease.
Dermatophytoses Severity Score – A novel point‐of‐care scoring tool to assess the severity of dermatophytosis
Authors: Bhat Ramesh M. , Madhumita Monisha , Jayaraman Jyothi 
Published: December 2022

AbstractThe rising prevalence of dermatophytosis in tropical countries coupled with drug resistance necessitates an objective scoring system to define the severity, monitor therapeutic response and predict prognoses. We attempted to establish and validate a new scoring system – Dermatophytoses Severity Score (DSS), for dermatophytoses affecting non‐glabrous skin. A consensus group was convened to develop an objective and reproducible scoring system to describe the extent and severity of dermatophytosis of 200 consecutive patients with dermatophytosis. A second assessment entailed independent DSS scoring of the same patients by dermatologists and residents who were not part of the consensus group. The main outcome measured was index reliability, assessed in two steps, between the observers. A two‐step assessment and DSS grading of 200 consecutive patients with clinically diagnosed dermatophytoses showed high reliability (Cronbach's α test and intraclass correlation coefficient). The DSS has demonstrated high reliability, and it could serve as a novel, reproducible and objective scoring tool for dermatophytosis.
Arteriolar neuropathology in cerebral microvascular disease
Authors: Fang Chuo , Magaki Shino D. , Kim Ronald C. , Kalaria Raj N. , Vinters Harry V. , Fisher Mark 
Published: December 2022

AbstractCerebral microvascular disease (MVD) is an important cause of vascular cognitive impairment. MVD is heterogeneous in aetiology, ranging from universal ageing to the sporadic (hypertension, sporadic cerebral amyloid angiopathy [CAA] and chronic kidney disease) and the genetic (e.g., familial CAA, cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy [CADASIL] and cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy [CARASIL]). The brain parenchymal consequences of MVD predominantly consist of lacunar infarcts (lacunes), microinfarcts, white matter disease of ageing and microhaemorrhages. MVD is characterised by substantial arteriolar neuropathology involving ubiquitous vascular smooth muscle cell (SMC) abnormalities. Cerebral MVD is characterised by a wide variety of arteriolar injuries but only a limited number of parenchymal manifestations. We reason that the cerebral arteriole plays a dominant role in the pathogenesis of each type of MVD. Perturbations in signalling and function (i.e., changes in proliferation, apoptosis, phenotypic switch and migration of SMC) are prominent in the pathogenesis of cerebral MVD, making ‘cerebral angiomyopathy’ an appropriate term to describe the spectrum of pathologic abnormalities. The evidence suggests that the cerebral arteriole acts as both source and mediator of parenchymal injury in MVD.
Mysterium Esse Christi: Thomas Aquinas & the Supernatural Being of Jesus Christ
Authors: Mabry Eric A. 
Published: December 2022

AbstractFor over 700 years scholastic theologians of varying degrees of allegiance to the text(s) of Thomas Aquinas have discoursed on the mystery of Christ's being (esse): Did Christ have one or two acts of existence? Yet despite this frequent and recurring quaestio, nevertheless only a handful of scholastic commentators pause to note that this is not simply a debate between rival scholastic ‘schools’ in regard to a theological mystery, but that in fact there is an inconsistency within the Angelic doctor's own texts. And while in more recent scholarship this discrepancy has not only been noticed but explicated in various ways, nevertheless it is the contention of this paper that a satisfactory exposition of the meaning of esse secundarium has not yet been achieved. Consequently, I propose in this paper that esse secundarium is the created, substantial, but absolutely supernatural participation of the human nature of Jesus in the uncreated communication of the divine esse of the Word and provide a robust textual defense of this interpretation.
Engineered Agrobacterium improves transformation by mitigating plant immunity detection
Authors: Yang Fan , Li Guangyong , Felix Georg , Albert Markus , Guo Ming 
Published: December 2022

Summary

Agrobacterium tumefaciens microbe‐associated molecular pattern elongation factor Tu (EF‐Tu) is perceived by orthologs of the Arabidopsis immune receptor EFR activating pattern‐triggered immunity (PTI) that causes reduced T‐DNA‐mediated transient expression. We altered EF‐Tu in A. tumefaciens to reduce PTI and improved transformation efficiency.

A robust computational pipeline was established to detect EF‐Tu protein variation in a large set of plant bacterial species and identified EF‐Tu variants from bacterial pathogen Pseudomonas syringae pv. tomato DC3000 that allow the pathogen to escape EFR perception. Agrobacterium tumefaciens strains were engineered to substitute EF‐Tu with DC3000 variants and examined their transformation efficiency in plants.

Elongation factor Tu variants with rarely occurred amino acid residues were identified within DC3000 EF‐Tu that mitigates recognition by EFR. Agrobacterium tumefaciens strains were engineered by expressing DC3000 EF‐Tu instead of native agrobacterial EF‐Tu and resulted in decreased plant immunity detection.

These engineered A. tumefaciens strains displayed an increased efficiency in transient expression in both Arabidopsis thaliana and Camelina sativa. The results support the potential application of these strains as improved vehicles to introduce transgenic alleles into members of the Brassicaceae family.
Coupling temperature‐dependent spatial turnover of microbes and plants using the metabolic theory of ecology
Authors: Xiao Xian , Ma Zhiyuan , Zhang Jiabao , Sun Bo , Zhou Jizhong , Liang Yuting 
Published: December 2022

Summary

There is an urgent need to understand the coupled relationship between belowground microbes and aboveground plants in response to temperature under climate change. The metabolic theory of ecology (MTE) provides a way to predict the metabolic rate and species diversity, but the spatial scale dependence and connections between plants and microorganisms are still unclear.

Here, we used two independent datasets to address this question. One is from comprehensive sampling of paddy fields targeting bacteria and microbial functional genes, and the other is a global metadata of spatial turnover for microorganisms (bacteria, fungi and archaea, n = 139) and plants (n = 206).

Results showed that spatial turnover of bacterial communities and microbial functional genes increased with temperature and fitted MTE. Through meta‐analysis, the temperature‐dependent spatial scale pattern was further extended to the global scale, with the spatial turnover of microorganisms and plants being consistent with MTE. Belowground microorganisms and aboveground plants were closely linked with each other even when controlling for temperature, suggesting that factors other than shared relationships with temperature also contribute to their linkages.

These results implied a broad application of MTE in biology and have important implications for predicting the ecological consequences of future climate warming.
AaMYB108 is the core factor integrating light and jasmonic acid signaling to regulate artemisinin biosynthesis in Artemisia annua
Authors: Liu Hang , Li Ling , Fu Xueqing , Li Yongpeng , Chen Tiantian , Qin Wei , Yan Xin , Wu Zhangkuanyu , Xie Lihui , Kayani Sadaf‐llyas , Hassani Danial , Sun Xiaofen , Tang Kexuan 
Published: December 2022

Summary

Artemisinin, a sesquiterpene compound synthesized and stored in the glandular trichome of Artemisia annua leaves, has been used to treat malaria. Previous studies have shown that both light and jasmonic acid (JA) can promote the biosynthesis of artemisinin, and the promotion of artemisinin by JA is dependent on light. However, the specific molecular mechanism remains unclear.

Here, we report a MYB transcription factor, AaMYB108, identified from transcriptome analysis of light and JA treatment, as a positive regulator of artemisinin biosynthesis in A. annua. AaMYB108 promotes artemisinin biosynthesis by interacting with a previously characterized positive regulator of artemisinin, AaGSW1.

Then, we found that AaMYB108 interacted with AaCOP1 and AaJAZ8, respectively. The function of AaMYB108 was influenced by AaCOP1 and AaJAZ8. Through the treatment of AaMYB108 transgenic plants with light and JA, it was found that the promotion of artemisinin by light and JA depends on the presence of AaMYB108.

Taken together, our results reveal the molecular mechanism of JA regulating artemisinin biosynthesis depending on light in A. annua. This study provides new insights into the integration of light and phytohormone signaling to regulate terpene biosynthesis in plants.
Pepper SnRK2.6‐activated MEKK protein CaMEKK23 is directly and indirectly modulated by clade A PP2Cs in response to drought stress
Authors: Jeong Soongon , Lim Chae Woo , Lee Sung Chul 
Published: December 2022

Summary

The phytohormone abscisic acid (ABA) is important for the plant growth and development, in which it plays a key role in the responses to drought stress. Among the core components of ABA signaling, SnRK2s interact with a range of proteins, including Raf‐like MAP3Ks.

In this study, we isolated the pepper MEKK subfamily member CaMEKK23 that interacts with CaSnRK2.6. CaMEKK23 has kinase activity and is specifically trans‐phosphorylated by CaSnRK2.6. Compared with control plants, CaMEKK23‐silenced pepper were found to be sensitive to drought stress and insensitive to ABA, whereas overexpression of CaMEKK23 in both pepper and Arabidopsis plants induced the opposite phenotypes. These altered phenotypes were established to be dependent on the kinase activity of CaMEKK23, which was also shown to interact with CaPP2Cs, functioning upstream of CaSnRK2.6.

In addition to inhibiting the kinase activity of CaMEKK23, these CaPP2Cs were found to have inhibitory effects on CaSnRK2.6. Using CaMEKK23‐, CaAITP1/CaMEKK23‐, CaSnRK2.6‐, and CaAITP1/CaSnRK2.6‐silenced pepper, we revealed that CaMEKK23 and CaSnRK2.6 function downstream of CaAITP1.

Collectively, our findings indicate that CaMEKK23 plays a positive regulatory role in the ABA‐mediated drought stress responses in pepper plants, and that its phosphorylation status is modulated by CaSnRK2.6 and CaPP2Cs, functioning as core components of ABA signaling.
Are Girls' and Boys' Cognitive Test Performance in Adolescence Differently Affected by Deprivation at Earlier Ages?*
Authors: Duc Le Thuc , Behrman Jere R. 
Published: December 2022

AbstractUsing data on the Millennium Children from the Young Lives Survey in Ethiopia, India, Peru, and Vietnam, we find that earlier nutritional growth and household wealth are important predictors of adolescent outcomes in math, reading, and receptive vocabulary for all children. Gender differences in the effect of wealth are significant mostly for non‐poor regions. The cognitive outcomes at age 8 are more strongly associated with growth between ages 1 and 5 for girls than boys. The gender differences reverse after age 8 mostly due to strong associations between growth in preadolescence ages and cognitive outcomes at age 15 for boys. Under the conditional mean independence assumption, the estimators for growth of the children are unbiased and consistent.
Focal melanocytic lesions of the oral mucosa: An epidemiological and morphological study
Authors: Santana Thalita , Queiroz Aline , Gonçales Luara Maria Cerioni , Andrade Natália Silva , Trierveiler Marília 
Published: December 2022

AbstractObjectiveThis study aimed to analyse the clinical and histopathological characteristics of focal oral melanocytic lesions in a Brazilian reference service in Oral and Maxillofacial Pathology.Materials and MethodsA cross‐sectional study was conducted over an 18‐year period. Demographic data and clinical features were collected from the archives, and all biopsy specimens diagnosed as oral melanocytic lesions were retrieved and reviewed.ResultsWe identified 339 melanocytic lesions. Of these, 191 were melanotic macules, 112 melanocytic nevi, 14 mucosal lentigo simplex, 12 melanomas, 9 solar lentigos, and 1 melanoacanthoma. Lesions occurred mostly in white‐skinned (74.2%) women (65.2%). The main reported clinical aspect was the macule (67.4%), and the most affected site was the lip vermilion (25.4%), followed by the palate (22.9%). Melanomas were larger in size and were observed in older patients with an overall shorter time of onset. The most frequent subtypes of melanocytic nevi were intramucosal (44.6%), compound (24.1%), and blue nevus (20.5%). They showed a heterogeneous architectural pattern with the presence of the three cell types.ConclusionThe most frequent lesions are melanotic macule and nevus, especially the intramucosal subtype. Patients are usually white‐skinned women presenting a small, long‐lasting, macular lesion on the lip vermilion or palate.