bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disulfidptosis classification of pancreatic carcinoma reveals correlation with clinical prognosis and immune profile
Authors: Shi Jiangmin , Zhao Liang , Wang Kai , Lin Jieqiong , Shen Jianwei 
Published: February 2025

Abstract

Background
Disulfidptosis, a novel form of metabolism-related regulated cell death, is a promising intervention for cancer therapeutic intervention. Although aberrant expression of long‐chain noncoding RNAs (lncRNAs) expression has been associated with pancreatic carcinoma (PC) development, the biological properties and prognostic potential of disulfidptosis-related lncRNAs (DRLs) remain unclear.

Methods
We obtained RNA-seq data, clinical data, and genomic mutations of PC from the TCGA database, and then determined DRLs. We developed a risk score model and analyzed the role of risk score in the predictive ability, immune cell infiltration, immunotherapy response, and drug sensitivity.

Results
We finally established a prognostic model including three DRLs (AP005233.2, FAM83A-AS1, and TRAF3IP2-AS1). According to Kaplan–Meier curve analysis, the survival time of patients in the low-risk group was significantly longer than that in the high-risk group. Based on enrichment analysis, significant associations between metabolic processes and differentially expressed genes were assessed in two risk groups. In addition, we observed significant differences in the tumor immune microenvironment landscape. Tumor Immune Dysfunction and Rejection (TIDE) analysis showed no statistically significant likelihood of immune evasion in both risk groups. Patients exhibiting both high risk and high tumor mutation burden (TMB) had the poorest survival times, while those falling into the low risk and low TMB categories showed the best prognosis. Moreover, the risk group identified by the 3-DRLs profile showed significant drug sensitivity.

Conclusions
Our proposed 3-DRLs-based feature could serve as a promising tool for predicting the prognosis, immune landscape, and treatment response of PC patients, thus facilitating optimal clinical decision-making.
Identification of subtypes and biomarkers associated with disulfidptosis-related ferroptosis in ulcerative colitis
Authors: Jiang Yinghao , Meng Hongyan , Zhang Xin , Yang Jinguang , Sun Chengxin , Wang Xiaoyan 
Published: February 2025

Abstract

Background
Disulfidptosis and ferroptosis are different programmed cell death modes, which are closely related to the development of a variety of diseases, but the relationship between them and ulcerative colitis (UC) is still unclear. Therefore, our study aimed to explore the molecular subtypes and biomarkers associated with disulfidptosis-related ferroptosis (DRF) in UC.

Methods
We used Pearson analysis to identify DRF genes. Then, we classified 140 UC samples into different subtypes based on the DRF genes and explored the biological and clinical characteristics between them. Next, the hub genes were identified by differential analysis and WGCNA algorithms, and three machine learning algorithms were used to screen biomarkers for UC from hub genes. In addition, we analyzed the relationship between biomarkers of immune cells and transcription factors and predicted natural compounds that might be used to treat UC. Finally, we further verified the reliability of the markers by RT-qPCR experiments.

Results
118 DRF genes were identified using Pearson analysis. Based on the expression level of the DRF genes, we classified UC patients into C1 and C2 subtypes, with significant differences in the abundance of immune infiltration and disease activity between the two subtypes. The machine learning algorithms identified three biomarkers, including XBP1, FH, and MAP3K5. Further analyses revealed that the three biomarkers were closely associated with a variety of immune cells and transcription factors. In addition, six natural compounds corresponding to the biomarkers were predicted, which may contribute to the effective treatment of UC. Finally, the expression trends of XBP1, FH, and MAP3K5 in animal experiments were consistent with the results of bioinformatics analysis.

Conclusion
In this study, we systematically elucidated the role of DRF genes in the development of UC, and identified three potential biomarkers, providing a new idea for the diagnosis and treatment of UC.
3D cinematic reconstructions of cardiovascular CT presented in augmented reality: subjective assessment of clinical feasibility and potential use cases
Authors: Böttcher Benjamin , van Assen Marly , Fari Roberto , von Knebel Doeberitz Philipp L. , Gershon Gabrielle , Meinel Felix G. , De Cecco Carlo N. 
Published: February 2025

Abstract
Augmented reality (AR) is a new technique enabling interaction with three-dimensional (3D) holograms of cinematic rendering (CR) reconstructions. Research in this field is in its very early steps, and data is scarce. We evaluated image quality, usability, and potential applications of AR in cardiovascular image datasets. Ten CR reconstructions of cardiovascular computed tomography (CT) datasets with complex anatomical abnormalities were presented to six radiologists and three cardiologists first on diagnostic screens and subsequently in AR. Subjective image quality and user experience were rated on 5-point Likert scales to assess usability and potential applications of AR. CR of CT datasets covering multiple images series of the same exam with differing kernels was performed in 143 ± 31 s (mean ± standard deviation); reconstruction of single CT image series took 84 ± 30 s. Mean subjective image quality was excellent, and observers showed high endorsement of the intuitive usability of the AR device and improvement of anatomical comprehensibility. AR devices were expected to have the greatest impact on patient and student education as well as multidisciplinary discussions, with less potential in clinical care. Clinical testing and preclinical implementation of AR seem feasible due to reasonable computation times and intuitive usability even for first-time users.

Relevance statement
The presentation of 3D cinematic rendering in augmented reality provides excellent image quality, facilitating the comprehension of anatomical structures in CT datasets. Concurrently, reasonable computation times and the intuitive usability of augmented reality devices make preclinical implementation and clinical testing feasible.

Key Points

3D cinematic reconstructions presented in augmented reality improve the anatomical comprehensibility of chest CT scans.

Augmented reality devices are expected to be highly beneficial in educational settings and multidisciplinary discussions.

Usability and computation times are feasible for initial preclinical use cases.

Graphical Abstract
Understanding the transfer and persistence of antimicrobial resistance in aquaculture using a model teleost gut system
Authors: Barcan Alexandru S. , Humble Joseph L. , Kasaragod Sandeep , Sajib Mohammad Saiful Islam , Barcan Rares A. , McGinnity Philip , Welch Timothy J. , Robertson Brendan , Vamanu Emanuel , Bacigalupo Antonella , Llewellyn Martin S. , Samsing Francisca 
Published: February 2025

Abstract

Background
The development, progression, and dissemination of antimicrobial resistance (AMR) are determined by interlinked human, animal, and environmental drivers, which pose severe risks to human and livestock health. Conjugative plasmid transfer drives the rapid dissemination of AMR among bacteria. In addition to the judicious use and implementation of stewardship programs, mitigating the spread of antibiotic resistance requires an understanding of the dynamics of AMR transfer among microbial communities, as well as the role of various microbial taxa as potential reservoirs that promote long-term AMR persistence. Here, we employed Hi-C, a high-throughput, culture-free technique, combined with qPCR, to monitor carriage and transfer of a multidrug-resistent (MDR) plasmid within an Atlantic salmon in vitro gut model during florfenicol treatment, a benzenesulfonyl antibiotic widely deployed in fin-fish aquaculture.

Results
Microbial communities from the pyloric ceaca of three healthy adult farmed salmon were inoculated into three bioreactors simulating the teleost gut, which were developed for the SalmoSim gut system. The model system was then inoculated with the Escherichia coli strain ATCC 25922 carrying the plasmid pM07-1 and treated with florfenicol at a concentration of 150 mg/L in fish feed media for 5 days prior to the washout/recovery phase. Hi-C and metagenomic sequencing identified numerous transfer events, including those involving gram-negative and gram-positive taxa, and, crucially, the transfer and persistence of the plasmid continued once florfenicol treatment was withdrawn.

Conclusions
Our findings highlight the role of the commensal teleost gut flora as a reservoir for AMR even once antimicrobial selective pressure has been withdrawn. Our system also provides a model to study how different treatment regimens and interventions may be deployed to mitigate AMR persistence.
Consistently low serum levels of MG-H1 is associated with a lower risk of diabetic kidney disease
Authors: Nakamura Tomoka , Tsujimoto Tetsuro , Yasuda Kazuki , Kajio Hiroshi , Ueki Kohjiro 
Published: February 2025

Abstract

Context
Diabetic kidney disease (DKD) is associated with an increased risk of cardiovascular events, end-stage renal disease, and mortality. Advanced glycation end products (AGEs) are related to DKD. However, data on the associations between long-term changes in AGEs and DKD are lacking.

Objective
We aimed to ascertain whether a long-term shift in serum AGE levels is associated with DKD development and progression in patients with poorly controlled diabetes.

Methods
The serum levels of the AGE, methylglyoxal-hydroimidazolone (MG-H1) were measured twice in 160 patients with diabetes. We categorized patients whose serum MG-H1 levels were &lt;2.5 µg/mL at both measurements as the consistently low MG-H1 group. The primary endpoints were new or worsening DKD, which was defined as the occurrence of either a 30% decline in estimated glomerular filtration rate (eGFR), doubling of serum creatinine, development of macroalbuminuria, need for renal replacement therapy, or death due to renal disease. Hazard ratios (HRs) for new or worsening DKD, with 95% confidence intervals (CIs), were calculated using Cox proportional hazard models to compare the outcomes between the consistently low MG-H1 group and the other group.

Results
Compared to the other group, the consistently low MG-H1 group had a significantly lower risk of new or worsening DKD, after adjusting for possible confounders (HR: 0.48; 95% CI, 0.29–0.81; P = 0.01). Furthermore, the same relationship was observed in patients without eGFR &lt;30 mL/min/1.73 m2, advanced DKD, or cardiovascular events.

Conclusions
Consistently low serum MG-H1 levels are associated with a lower frequency of DKD.
Azoospermia/Oligozoospermia and Prostate Cancer are Increased in Families of Women with Primary Ovarian Insufficiency
Authors: Allen-Brady Kristina , Kodama Samantha , Verrilli Lauren E , Ramsay Joemy M , Johnstone Erica B , Horns Joshua J , Emery Benjamin R , Cannon-Albright Lisa , Aston Kenneth I , Hotaling James M , Welt Corrine K 
Published: February 2025

Abstract

Background
Nonobstructive azoospermia (NOA) and primary ovarian insufficiency (POI) have common genetics that may also predispose to cancer risk.

Objectives
We hypothesized that NOA or severe oligozoospermia and risk of male cancers would be higher in families of women with POI.

Methods
Women with POI were identified using International Classification of Disease codes in electronic medical records (1995-2021) from two major healthcare systems in Utah and reviewed for accuracy. Using genealogy information in the Utah Population Database, women with POI (n=392) and their relatives were included if there were at least three generations of ancestors available. Men with NOA or severe oligozoospermia (≤5 million/mL) from the Subfertility Health and Assisted Reproduction and the Environment Study were identified in these families and risk was calculated in relatives compared to population rates. The relative risk of prostate and testicular cancer was examined using the Utah Cancer Registry.

Results
There was an increased risk of NOA/severe oligozoospermia in relatives of women with POI among first- (RR 2.8 [95% CI 1.1, 6.7]; p=0.03), second- (3.1 [1.1, 6.7]; p=0.02), and third-degree relatives (1.8 [1.1, 3.1]; p=0.03). In these families with POI and NOA/oligozoospermia (n=21), prostate cancer risk was higher in first- (3.5 [1.1, 8.1]; p=0.016) and second-degree relatives (3.1 [1.9, 4.8]; p=0.000008).

Conclusions
The data demonstrate excess familial clustering of severe spermatogenic impairment compared to matched population rates, along with higher prostate cancer risk in relatives of women with POI. These findings support a common genetic contribution to POI, spermatogenic impairment and prostate cancer.
EFEKTIVITAS VIDEO EDUKASI TERHADAP PENGETAHUAN DAN SIKAP REMAJA DALAM MENCEGAH PERKAWINAN USIA ANAK
Authors: Adhiyanti Iis Putri , Mediastuti Fitriani , Hindriyawati Wiwin , Setyorini Retno Heru 
Published: February 2025

Penelitian ini bertujuan untuk mengevaluasi dampak video edukasi dalam meningkatkan pengetahuan dan sikap remaja dalam mencegah perkawinan usia anak. Perkawinan usia anak merupakan salah satu permasalahan remaja yang mengancam terwujudnya hak-hak dasar anak. Tingginya kasus perkawinan anak dapat disebabkan oleh kurangnya pengetahuan remaja, yang menyebabkan remaja bersikap negatif. Pengetahuan dan sikap remaja tentang perkawinan anak dapat ditingkatkan melalui penyuluhan menggunakan video edukasi.
Desain penelitian ini adalah quasi eksperimen dengan one group pretest and posttest design. Sampel penelitian berjumlah 55 siswa dengan teknik pengambilan sampel menggunakan simple random sampling. Analisis data dalam penelitian ini menggunakan uji Wilcoxon. Hasil penelitian menunjukkan bahwa terdapat pengaruh yang signifikan dari intervensi video edukasi yang diberikan, dimana terjadi peningkatan pengetahuan dan sikap tentang perkawinan usia anak (p-value < 0,001).
Intervensi melalui video edukasi dapat meningkatkan pengetahuan dan sikap remaja tentang perkawinan usia anak..
Design of a vertically stacked double Luneburg lens-based beam-scanning antenna at 60 GHz
Authors: Alhumaidan Ahmed , Vettikalladi Hamsakutty , Bahammam Meshal , Alrashied Tariq , Alkanhal Majeed 
Published: February 2025

Abstract
This article presents the design of a 60 GHz beam-scanning antenna system with a coverage range of 50–100 m for high-speed indoor communication systems. The design incorporates beam-scanning techniques to meet the coverage requirements while minimizing system cost and size. The system comprises a substrate-integrated waveguide for stable operation, a printed log periodic dipole array operating at 60 GHz with a 10 GHz bandwidth (54–67 GHz), which provides a 6.99 dB directivity. Adding a vertically stacked double Luneburg lens improves pattern coverage performance and increases the system directivity to 17 dBi (14.8 dB gain) with a 4.6° beam width. With 37 source antennas, it can provide a total beam coverage area of 170°, which is highly suitable for future high-speed indoor communication.
Synergistic effect of nano-silica, steel slag, and waste glass on the microstructure, electrical resistivity, and strength of ultra-high-performance concrete
Authors: Mohammed Abbas J. , Hassan Maan S. , Al-Quraishi Hussein 
Published: February 2025

Abstract
This study examined the microstructure characteristics, electrical resistivity (ER), and mechanical properties of ultra-high-performance concrete (UHPC) made with nano-silica (NS), electric arc furnace slag (EAFS), and waste glass powder (WGP). Eight concrete mixtures of binary and ternary blends were assessed to investigate the possible relationships between the observed physical and microstructural features with the obtained compressive and flexural strengths. The individual and combined effects of NS, EAFS (up to 50% replacement of silica sand), and WGP (up to 30% cement replacement) on the examined properties were evaluated. Scanning electron microscopy (SEM), thermogravimetric (TG) analysis, X-ray diffraction (XRD), ER, and pH values, and measurements were employed. SEM examination indicated that using NS along with EAFS and WGP reduced gap space around the fiber, and the enormous single cracks turned into numerous small cracks, thereby improving bonding and flexural strength. TG and XRD studies supported this finding. The ER for the EAFS specimens was enhanced without compromising the alkalinity range of concrete, as proved by pH tests, which address the possible corrosion problem of reinforcement. Thus, sustainable UHPC is achievable.
Graphene-based tunable dual-band polarization sensitive absorber for applications in the terahertz regime
Authors: Mazaheri Muhammad Hani , Maab Husnul , Rahim Arbab Abdur 
Published: February 2025

Abstract
This paper proposes a tunable dual-band polarization-sensitive absorber that displays two distinct absorptance peaks for two different polarized incident lights. The proposed absorber uses graphene as the material for the metasurface to utilize its tunable surface conductivity for achieving a tunable resonant frequency. The tunable absorber displayed two distinct absorption peaks for two separately polarized incident waves. For x-polarized incident light, the proposed absorber displayed perfect absorptance for a tunable bandwidth of 3 THz between 2.85 THz and 5.85 THz, and for y-polarized incident light, the proposed absorber displayed perfect absorptance for a tunable bandwidth of 1.5 THz between 11 THz and 12.5 THz. Numerical computations were performed with extremely fine mesh quality to study the absorptance spectrum of the proposed absorber for different Fermi energy levels, incident angles, and azimuthal angle of incidence. The proposed absorber displays appreciative characteristics which makes it a suitable candidate for energy harvesting, polarization detection, and polarization conversion of unpolarized light.
k-tuple total dominator chromatic number and Mycielskian graphs
Authors: Marweni Walid 
Published: February 2025

Abstract
The k-tuple total dominator chromatic number is a graph parameter that measures the minimum number of colors required for a proper coloring, where each vertex must be adjacent to every vertex of k distinct color classes.
In this paper, we investigate the k-tuple total dominator chromatic number in the context of Mycielskian graphs.
Mycielskian graphs are obtained through a graph transformation introduced by Mycielski in 1955, and they have been widely studied for their intriguing properties and applications in graph theory. The generalized Mycielskians are natural generalizations of Mycielskian graphs, denoted by

μ
m

⁢

(
G
)

{\mu_{m}(G)}

, where

m
≥
1

{m\geq 1}

. Specifically, we determine both the upper and lower bounds for the k-tuple total dominator chromatic number of Mycielskian graphs, providing insights into the minimum number of colors needed to achieve a k-tuple total dominator coloring in these graphs. We also discuss the exact values of the k-tuple total dominator chromatic number for specific instances of Mycielskian graphs. Moreover, we discuss the

χ

k
,
d

t

{\chi_{k,d}^{t}}

of the generalized Mycielskian graph

μ
m

⁢

(
G
)

{\mu_{m}(G)}

, where

m
≥
2

{m\geq 2}

, by giving some bounds in terms of

k
,
m

{k,m}

and that of G.
Chinese perceptions and refutations of face-threatening impoliteness regarding diplomatic press conferences
Authors: Zhang Cun , Liu Guiling , Zhang Shuang 
Published: February 2025

Abstract
Academic interest in impoliteness within political interactions has grown, but there remains a significant gap in the exploration of Chinese political discourse, let alone understanding how and why multiple participants perceive and respond to this phenomenon. Moreover, metaphor, as a crucial off-record strategy in impoliteness research, has not been given in-depth scrutiny by pragmaticists. To address the gaps, we collected 228 Chinese diplomatic press conference texts, and interviewed 27 Chinese citizens to quantitatively and qualitatively analyze external journalists’ face-threatening impoliteness and public understandings and retorts, in comparison with Chinese spokespersons’ measures of counter-impoliteness. It is found that first, the journalists’ impoliteness primarily consists of explicit negative association, seeking disagreement, and threatening/frightening. Second, the interviewees’ evaluation of impoliteness is influenced by their background knowledge, collective identity, political stances, and attention to the journalists’ querying manners. Third, the spokespersons use 11 types of deliberate metaphors along with other negative evaluations such as denials and rhetorical questions. Apart from these devices, the interviewees’ responses abound with distinct outpourings of other-condemning sentiment. The multiple recipients’ judgments and rebuttals may interrelate with their discoursal and social roles, which are arguably affected by China’s contemporary diplomacy and nationals’ mentalities.
Rearing in an envy-like environment increases anxiety-like behaviour in mice
Authors: Ueno Hiroshi , Kitano Eriko , Takahashi Yu , Mori Sachiko , Murakami Shinji , Wani Kenta , Matsumoto Yosuke , Okamoto Motoi , Ishihara Takeshi 
Published: February 2025

Abstract
Interest in the societal and psychological harm caused by widespread envy and social comparison is increasing. Envy is associated with anxiety and depression, though the mechanism by which envy affects neuropsychiatric disorders, such as depression, remains unclear. Clarifying the neurobiological basis of envy’s effects on behaviour and emotion regulation in experimental mice is essential for developing disease-prevention and treatment strategies. As mice recognize other mice in neighbouring cages, this study investigated whether they recognize neighbouring cages housed in environmentally enriched cages and suffer psychological stress due to envy. After being raised in an envy-like environment for 3 weeks, we revealed changes in the behaviour of the mice through a series of behavioural experiments. Mice raised in an envious environment showed increased body weight and anxiety-like behaviour but decreased social behaviour and serum corticosterone levels compared to control mice. Thus, mice recognize their neighbouring cages and experience psychological stress due to envy. This study revealed a part of the scientific basis for why envy increased anxiety. Using this novel experimental breeding environment, it may be possible to create an experimental animal model of anxiety disorders.