bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resonance assignments of the PUB domain of the RNF31 protein
Authors: Song Lanlan , Zhong Fumei , Zhang Jiahai , Tu Xiaoming 
Published: February 2023

Abstract
RNF31 is identified in human proteins and is involved in activities of the linear ubiquitin chain assembly complex (LUBAC) and cell growth. RNF31 plays an important role in LUBAC-mediated ubiquitination. Here, we report the backbone and side-chain resonance assignments of the PUB domain of RNF31 and study the backbone relaxation of the domain. These studies will contribute to further understanding of the structure and function relationship of the RNF31 protein.
CircAkt3 participates in bone cancer pain progression in rats by modulating MAPK signalling pathway
Authors: Chen Lvlv , Zhang Xiaoxia , Su Yuqi , Tang Tianying , Chen Hanwen , Alam Azeem , Kamath Priyanka , Chen Qian , Zhao Hailin , Zhang Yiwen 
Published: February 2023

Abstract
Background: Cancer-induced bone paincaused by advanced tumor bone metastasis remains a clinical challenge, and the underlying mechanisms of BCP remain unknown. This study aimed to screen the expression profile of circular RNAs in a BCP rat model and provide a new theoretical basis for the role of circular RNA in the occurrence and development of BCP.
Methods: We established a BCP rat model. The top four differentially expressed circRNAs (DECs) in the model were validated by agarose gel electrophoresis and Sanger sequencing between the BCP group and sham group. A circRNA-miRNA-mRNA network was constructed based on the interactions among circRNAs, microRNA (miRNA), and mRNA, which were predicted by TargetScan. mRNA and circRNA expression levels were detected by quantitative RT-PCR. In addition, Western Blot was performed to identify the protein levels of p-ERK, ERK, and Col8a1.
Results: CircRNA parent genes were mainly enriched in MAPK and neurodevelopmental signalling pathways. CircAkt3 and circMap4k1 were significantly up-regulated in the BCP group. CircaAkt3 may increase p-ERK expression by upregulating Col8a1, which may further activate the MAPK pathway.
Conclusions: The circAkt3 pathway may influence the development of bone cancer pain by activating the MAPK signaling pathway. This study provided important targets for the development of therapeutic strategy against BCP.
Intraradicular diameter, remnant dentin thickness, and endodontic post-to-dentin distanc: An investigation using CBCT and microscopy
Authors: Souza Júlio C. M. , Soares Catarina , Fernandes Valter , Carvalho Oscar , Silva Filipe , Özcan Mutlu , Henriques Bruno 
Published: February 2023

Abstract
The objective of this work was to measure the tooth root canals’ diameter, remnant dentin thickness, and the endodontic post-to-dentin distance after three types of root canal preparation. Thirty extracted human premolars were endodontically treated and groups of specimens were divided according to the cementation with two different endodontic post as follow: A) Fibio Fiberglass Post™, Anthogyr, France; B) multi-filament GFRC (Rebilda GT™, VOCO, Germany). Cone beam computed tomography (CBCT) and conventional x-ray analyses were performed before and after the endodontic post cementation. After cementation, specimens were cross-sectioned and inspected by optical microscopy and scanning electron microscopy at magnification from x30 up to x2000. The shape variation of the root canal preparation caused a decrease in the thickness of the remnant tooth tissues. CBCT and microscopic analyses also revealed a clear variation of resin-matrix cement around all the endodontic GFRC posts. A multi-filament GFRC post provided a distribution of filaments although the resin-matrix cement revealed a high volume among the filaments. An increase in thickness and volume of resin-matrix cement was noticed at the coronal third since the fitting was compromised due to anatomic variations and root canal preparation. Defects such macro-scale pores, cracks, and voids were also detected by microscopic analyses. The root canal preparation can promote a decrease in the thickness of the remnant tooth tissues that can increase the risks of clinical failures by fracture. The thickness and volume of resin-matrix cement varied around both GFRC posts. Additionally, that increased from the apex up to the coronal third due to the lack of fitting. Clinical significance: The root canal preparation of teeth is the first step the fitting of glass fiber-reinforced composite (GFRC) post. However, the tooth tissues must be preserved to maintain the mechanical integrity of the restored tooth. An adequate fitting of the GFRC post along the intracanal regions can promote a well-distribution of the resin-matrix cement volume that decreases the risks of defects such as voids and cracks.
The effect of triage training using the WebQuest method on the knowledge level of nursing students in patient triage
Authors: Saeedi Maryam , Panahi Marzieh 
Published: February 2023

Abstract
Background: In conventional educational programs, learners are passive during instruction and are rarely given the opportunity to think. One of the student-centered educational methods that causes deep learning in students is the WebQuest method. which can create an important transformation in learners. Considering the limited studies in this field, the present study was conducted with the aim of comparing the effectiveness of lecture and web quest methods on the knowledge of nursing students in patient triage at Saveh University of Medical Sciences.
Method: This research is an interventional and semi-experimental study. The present study was conducted in Saveh University of Medical Sciences between 2021 and 2022. The participants included Sixty-eight nursing students of the fifth semester who were randomly assigned to intervention and control groups. The control group was trained by the instructor and the intervention group was trained by the 6-step web quest method. The triage Knowledge questionnaire was taken as a pre-test and post-test. Then the scores were analyzed using the Wilcoxon, Mann-Whitney and independent t-tests.
Results: The average score of students' knowledge about triage in the control and intervention groups, before the intervention, did not have a statistically significant difference (P=0.89). But after the intervention, the average score of students' knowledge about triage in the control and intervention groups had a statistically significant difference (P<0.001).
Conclusion: The results of this research showed that teaching triage using the web quest method causes enables students to learn more than the lecture method. Therefore, it is recommended to use the WebQuest method as one of the effective methods in triage training.
Sleep loss diminishes hippocampal reactivation and replay
Authors: Giri Bapun , Kaya Utku , Maboudi Kourosh , Abel Ted , Diba Kamran 
Published: February 2023

Abstract

Memories benefit from sleep, and sleep loss immediately following learning has a negative impact on subsequent memory storage. Several prominent hypotheses ascribe a central role to hippocampal sharp-wave ripples (SWRs), and the concurrent reactivation and replay of neuronal patterns from waking experience, in the offline memory consolidation process that occurs during sleep. However, little is known about how SWRs, reactivation, and replay are affected when animals are subjected to sleep deprivation. We performed long duration (~12 h), high-density silicon probe recordings from rat hippocampal CA1 neurons, in animals that were either sleeping or sleep deprived following exposure to a novel maze environment. We found that SWRs showed a sustained rate of activity during sleep deprivation, similar to or higher than in natural sleep, but with decreased amplitudes for the sharp-waves combined with higher frequencies for the ripples. Furthermore, while hippocampal pyramidal cells showed a log-normal distribution of firing rates during sleep, these distributions were negatively skewed with a higher mean firing rate in both pyramidal cells and interneurons during sleep deprivation. During SWRs, however, firing rates were remarkably similar between both groups. Despite the abundant quantity of SWRs and the robust firing activity during these events in both groups, we found that reactivation of neurons was either completely abolished or significantly diminished during sleep deprivation compared to sleep. Interestingly, reactivation partially rebounded upon recovery sleep, but failed to reach the levels characteristic of natural sleep. Similarly, the number of replays were significantly lower during sleep deprivation and recovery sleep compared to natural sleep. These results provide a network-level account for the negative impact of sleep loss on hippocampal function and demonstrate that sleep loss impacts memory storage by causing a dissociation between the amount of SWRs and the replays and reactivations that take place during these events.
Incorporating copper into fluoride-containing bioglasses enhances the inhibition of Streptococcus mutans activity and biofilm formation
Authors: Chen Xiaojing , Zou Songyou , Yan Yingcui , He Hailun , Liu Ousheng , Wang Songlin 
Published: February 2023

AbstractLow fluoride-containing bioglasses (LFBGs) with the ability to controllably degrade and form acid-resistant fluorapatite are attractive for caries prevention. However, their antibacterial effect is not satisfactory. To address this problem, we first time incorporated copper (0-5 mol%) into LFBG (1 mol% CaF2) by a melt-quench method. Then, we investigated the effects of copper addition on the glass structure, bioactivity, cytocompatibility, inhibitory performance againstStreptococcus mutansand the potential antibacterial mechanism of glasses. The results revealed that copper was not bound directly to fluoride or orthophosphate and might be present as Si-O-Cu2+. Glasses with the proper copper content (≤ 2 mol%) were cytocompatible. All of the Cu-doped LFBGs (FCuBGs) exhibited high bioactivity and excellent antibacterial properties with increasing copper content. Moreover, we present the first detailed analysis of the potential antibacterial mechanism of FCuBGs, which are attractive for dental applications, including use as fissure sealers, varnishes, and additives for caries prevention.
High-throughput sequencing reveals the change of TCR α chain CDR3 with Takayasu arteritis
Authors: Zha Bowen , Pan Lili , Gao Na 
Published: February 2023

Abstract
To research the immunological characteristics of antigen complementarity determining the region of T cell receptor α chain in peripheral blood of patients with Takayasu's arteritis, and to provide the new theoretical basis for the diagnosis and treatment of Takayasu's arteritis. Five untreated patients with Takayasu arteritis in Capital medical university affiliated Anzhen Hospital, China, were collected from June 2019 to December 2019. Four healthy peripheral blood samples were matched as the control group. Separate peripheral blood mononuclear cells and extract RNA for reverse transcription. Specific primers obtained nucleotides in the CDR3 region of the TCR α chain, high-throughput sequencing was performed, and the quality of samples was evaluated by principal component analysis. R software was used for statistical analysis and drawing, and a nonparametric test was used to analyze the differences between the two groups. The results of the principal component analysis showed that the TAK group was in the sample set. D50 analysis showed that the diversity of TCR in the TAK group was significantly higher than in the control group. The expression of 3 genes in the V region was significantly higher in patients than in the control group. 196 VJ rearrangement genes are significantly different between the two groups, of which 47 rearrangement genes in the control group are lower than those in the TAK group, and 149 rearrangement genes in the control group are higher than those in the TAK group. Patients with Takayasu's arteritis have a unique CDR3 library, and there are obvious disease-related T cell clones. These characteristic genes may be a marker for early diagnosis and provide a new theoretical basis for treating Takayasu arteritis.
Analysis of related factors influencing the sleep quality in the patients with rotator cuff tear after arthroscopic surgery
Authors: Zhang Qian , Li Yanjun , Li Yongwang , Wang Chunguang , Yao Yichao , Li Qiuping 
Published: February 2023

Abstract
Background: Recent studies have found a prevalence of sleep disturbance in patients with arthroscopic rotator cuff repair. The purpose of our study was to determine which patient-reported factors correlate with sleep disturbance in patients with arthroscopic rotator cuff repair.
Methods: We prospectively evaluated 133 patients who had had arthroscopic rotator cuff repaired for 6 months. We obtained the Pittsburgh Sleep Quality Index (PSQI) scores, the visual analog scale (VAS) pain score, the University of California-Los Angeles Shoulder Rating Scale score (UCLA score), the Hospital Anxiety and Depression Scale (HADS), and patient demographic. According to PSQI scores, participants were divided into a sleep disorder group ( A group, PSQI ≥ 5 ) and a normal sleep group (B group, PSQI < 5). Statistical analysis included the Student t-tests, Mann-Whitney U-test，chi-square tests, and binary logistic regression analysis to determine which patient-reported factors were associated with sleep disturbance.
Results: The mean scores on the VAS, UCLA Score, UCLA Flexion, HADS-Anxiety (HADS-A), and HADS-Depression(HADS-D) in group A were 3.54, 26.36, 3.25, 5.43, and 5.93, respectively; while in group B were 1.49, 30.72, 4.50, 2.11, and 1.79, respectively. There was a statistically significant difference for scores on the VAS, UCLA, UCLA Flexion, HADS-A, HADS-D between the 2 groups (P < 0.05). In the categories of gender, age, BMI, and tear size, there was no statistically significant difference between the two groups. (P > 0.05). HADS-D and UCLA Flexion were independent factors affecting sleep disturbance after arthroscopic rotator cuff repair 6 months (P < 0.05).
Conclusions: Our study demonstrated patients with sleep disturbances after arthroscopic shoulder surgery had severe pain, more obvious dysfunction, and more pronounced psychologic abnormalities, and the close relationships of depression, UCLA Flexion scores, and sleep disturbance may indicate that more emphasis on psychotherapy and rehabilitation is required.
Empirical Mode Decomposition and Wavelet Decomposition in Respiratory Sounds Processing
Authors: Han Chengwei , Wang Yixuan , Sun Zhibo , Fei Xie 
Published: February 2023

Abstract
In order to process respiratory sounds to achieve acquiring available information of sound signals Empirical Mode Decomposition (EMD) and Wavelet Decomposition (WD) are applied separately to analyze lung sound signals. The de-noise of original signals is processed with spectral subtraction method. EMD divides the signal into independent Intrinsic Mode Functions (IMFs). Moreover, WD method decomposes the signal with wavelet transform. After receiving the decomposition signals of EMD and WD, a comparation is demonstrated. According to the results, WD has 85% signal information concentrate on layer 7 and Hilbert diagram shows the EMD owns more efficiency in decomposing signals with information keeping.
Anti-microbial activity and biofilm inhibition of Streptococcus mutans by Coriandrum sativum essential oil and isothiocyanates
Authors: Ngalah Bidii S. , Hansen Angela , Uwe Frank , Alexandru Movila , Gregory Richard L. 
Published: February 2023

AbstractAims This study evaluated the antimicrobial effect of coriander oil (CO) and isothiocyanates (ITC; Allyl, Benzyl and Phenyl-A-, B-, P-ITC) on clinical oral isolateStreptococcus mutans. We evaluated inhibition of biofilm, metabolic activity and exopolysaccharide formation. Methods The composition of CO was determined by gas chromatography- mass spectrometry (GC-MS). CO and ITC were dissolved in 0.1% Tween 80. Different concentrations (0.0039-1%) of CO, ITC and CO-ITC were made in Tryptic soy broth (TSB) and with sucrose (TSBS). Broth dilution method was used to determine the minimum inhibitory concentration (MIC). Minimum biofilm inhibitory concentrations (MBIC) were established by staining biofilm with crystal violet. Checkerboard assay was used to evaluate combination effects. An XTT assay was used to determine the metabolic activity and a sulphuric acid-phenol assay for inhibition ofS. mutansexopolysaccharide production. Results CO was dominated by Linalool (65.5%) followed by 2-bornanone (6.16%) and Gamma-terpinene (4.31%). The least was Terpinen-4-ol (0.13%). The MIC of CO was 0.00195% and < 0.00195% for ITCs. MBIC for B-ITC was at < 0.0039%, while with CO-ITC at 0.00195% (p < 0.05). The MBC of CO was 0.125% and for CO-B-ITC MBIC was < 0.00195%. The B-ITC MBC was > 0.25%. StrongS. mutansexopolysaccharide inhibition was observed with ITC and CO at 0.0039%. CO reducedS. mutansmetabolic activity at 0.125% and ITC at 0.0078%. Conclusion and clinical relevance: CO and ITC are promising agents in prevention of both periodontal disease and dental caries. Further data is required to warrant development of products to help mitigate dental caries and periodontal disease.
EvidenceCap: Towards trustworthy medical image segmentation via evidential identity cap
Authors: Zou Ke , Yuan Xuedong , Shen Xiaojing , Chen Yidi , Wang Meng , Goh Rick Siow Mong , Liu Yong , Fu Huazhu 
Published: February 2023

Abstract
Medical image segmentation (MIS) is essential for supporting disease diagnosis and treatment effect assessment. Despite considerable advances in artificial intelligence (AI) for MIS, clinicians remain skeptical of its utility, maintaining low confidence in such black box systems, with this problem being exacerbated by low generalization for out-of-distribution (OOD) data. To move towards effective clinical utilization, we propose a foundation model named EvidenceCap, which makes the box transparent in a quantifiable way by uncertainty estimation. EvidenceCap not only makes AI visible in regions of uncertainty and OOD data, but also enhances the reliability, robustness, and computational efficiency of MIS. Uncertainty is modeled explicitly through subjective logic theory to gather strong evidence from features. We show the effectiveness of EvidenceCap in three segmentation datasets and apply it to the clinic. Our work sheds light on clinical safe applications and explainable AI, and can contribute towards trustworthiness in the medical domain.
m6A demethylase FTO stabilizes LINK-A to exert oncogenic roles via MCM3-mediated cell cycle progression and HIF-1α activation
Authors: Liu Zhihua , Nan Yabing , Liu Shi , Luo Qingyu , Wu Xiaowei , Zhao Pengfei , Chang Wan , Zhang Ruixiang , Li Yin 
Published: February 2023

AbstractRNAN6-methyladenosine (m6A) modification, balanced by methyltransferases and demethylases, has recently been shown to play critical roles in multiple cancers. However, the mechanism by which m6A modification regulates long noncoding RNA (lncRNA) stability and function during cancer progression remains unclear. Here, we show that m6A demethylase fat mass and obesity-associated protein (FTO) removes the m6A modification on long intergenic noncoding RNA for kinase activation (LINK-A) and stabilizes it to promote cell proliferation and cytotoxic chemotherapy resistance in esophageal squamous cell carcinoma (ESCC). Mechanistically, LINK-A enhances the interaction between minichromosome maintenance complex component 3 (MCM3) and cyclin-dependent kinase 1 (CDK1) to promote MCM3 phosphorylation by CDK1. MCM3 is a subunit of the hexameric protein complex and its phosphorylation facilitates loading of the MCM complex onto chromatin, which promotes cell cycle progression and subsequent cell proliferation. Meanwhile, LINK-A prevents the interaction of MCM3 and hypoxia-inducible factor 1α (HIF-1α), abrogates MCM3-mediated transcriptional repression of HIF-1α, and promotes glycolysis and chemoresistance of cancer cells. These results elucidate a mechanism whereby FTO-stabilized LINK-A plays oncogenic roles and present the FTO/LINK-A/MCM3/HIF-1α axis as a promising therapeutic target for ESCC.
Modified carboxymethyl cellulose nanocrystalline imprinted aerogels with three-dimensional interoperable pore structure for selective adsorption of gadolinium
Authors: Li Ang , Zheng Xudong , Ji Biao , Xu Tongtong , Zhang Yuzhe , Rong Jian , Mei Jinfeng , Zhou Man , Li Zhongyu 
Published: February 2023

Abstract
Rare earth elements are widely exploited with the deepening of the dual carbon strategy, and it becomes especially important to recover residual rare earth elements as a strategic resource. In the present experiments, we have found that silk gliadin has a variety of strongly polar side chains that allow easy cross-linking, copolymerization and blending with other polymers, and in addition we have found that sodium alginate can form honeycomb-like porous structures at certain ratios. Therefore, it is proposed to use silk glue protein and sodium alginate as precursors to functionalize modified cellulose nanocrystals, combined with ion imprinting technique to construct porous imprinted aerogels and apply them to the selective adsorption of gadolinium ions. The successful synthesis of the material was demonstrated by relevant characterization, and the results of static adsorption experiments showed that the maximum adsorption capacity was 93.41 mg g− 1 at pH = 7.0. Sodium alginate provides a stable honeycomb 3D structure and silk gum provides a large number of adsorption sites, providing a viable direction for green porous sustainable adsorbent materials.
Spatial-temporal Evolution and Influencing Factors of Carbon Emissions in China's Manufacturing Industry
Authors: Zeng Qian , Cao Shuya , He Jiayi 
Published: February 2023

Abstract
Manufacturing carbon emissions account for a huge proportion of China 's total carbon emissions, and promoting manufacturing carbon emission reduction will effectively help achieve the ' double carbon ' goal. This paper explores the spatial and temporal evolution characteristics and changing trends of carbon emissions in China 's manufacturing industry by industry and province through exploratory spatial-temporal data analysis method and spatial-temporal transition measurement method, and summarizes the absolute β convergence and conditional β convergence trend of China 's manufacturing industry at the national level and in the eastern, central and western regions. On this basis, the spatial Dubin model is used to analyze the influence degree and spatial effect of economic growth, energy intensity and energy structure on carbon emissions in China 's manufacturing industry. The results show that the emission reduction effect of sub-sectors is not the same. Compared with technology-intensive industries, the carbon reduction effect of resource-intensive industries is poor. From the provincial perspective, the spatial distribution of manufacturing carbon emissions among provinces and cities in China shows the spatial and temporal evolution characteristics of agglomeration and differentiation. There are absolute β convergence and conditional β convergence in the whole country and the eastern, central and western regions From the perspective of convergence speed, the convergence speed of the central region is faster than that of the western region, and the western region is faster than the eastern region ; economic growth, energy intensity, urbanization level, energy structure and openness have a significant positive impact on the level of manufacturing carbon emissions in the province and neighboring provinces and cities, and the degree of influence decreases in turn. Industrial structure has a significant negative impact on the level of manufacturing carbon emissions in the province and neighboring provinces and cities.