bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of osteoarthritis-characteristic genes and immunological micro-environment features by bioinformatics and machine learning
Authors: Da Zheng , Guo Rui , Sun Jianjian , Wang Ai 
Published: May 2023

Abstract
Background
Osteoarthritis (OA) is a mechanistically complex chronic joint disease which will reduce the life quality of middle-aged and elderly people as well as increase the socioeconomic burden. Currently, the pathophysiology of OA is not entirely clear. The purpose of this study was to investigate the genes, functional pathways, as well as characteristics of immune infiltration, that are involved in the genesis and progression of osteoarthritis.
Methods
The GEO database was used to obtain gene expression profiles. R software was used for the screening of differentially expressed genes (DEGs) and enrichment analysis of these genes. OA characteristic genes were screened by WGCNA and the Lasso algorithm. Using ssGSEA, we evaluated the infiltration levels of immune cells in cartilage, followed by correlation analysis between immune cells and OA characteristic genes.
Results
We identified 80 DEGs in total. Results of the functional enrichment indicated that these DEGs were associated with chondrocyte metabolism, apoptosis, and inflammation. Three OA characteristic genes were identified by WGCNA analysis and the lasso algorithm, and then their expression levels were verified by the verification set. Finally, immune cells infiltration analysis revealed that T cells and B cells were mainly associated with OA. In addition, Tspan2, HtrA1 showed correlation with some of the infiltrating immune cells.
Conclusions
The results of a comprehensive bioinformatic analysis showed OA is associated with a number of characteristic genes, functional pathways, immune cell infiltration processes. Characteristic genes and functional pathways identified in this study can be used as biomarkers to guide drug treatment and molecular-level research on OA.
The effect of arterial spin labeling MR angiography (ASL-MRA) in visualizing the branches of external carotid artery
Authors: Yogi Akira , Ito Junji , Ishikawa Kazuki , Heianna Joichi , Sakugawa Satoshi , Aguni Narihisa , Obara Makoto , Maeda Hiroyuki , Nishie Akihiro 
Published: May 2023

Abstract
This study aimed to assess the performance of arterial-spin labeling MRA (ASL-MRA) for visualizing the external carotid artery (ECA) branches in comparison with time-of-flight MRA (TOF-MRA) and CT angiography (CTA). We retrospectively selected 31 consecutive patients, who underwent both MRAs and CTA, prior to the intra-arterial chemoradiotherapy (IACRT) for head and neck cancer. Four patients underwent IACRT bilaterally, so we analyzed 35 ECAs. Pseudo-continuous, three-dimensional ASL using a turbo field echo sequence was acquired. For the TOF-MRA and CTA, clinically used parameters were applied. Two observers evaluated each ECA branch with reference to the angiogram at the IACRT, using five-point scale, in consensus. Friedman test for multiple comparisons was applied. ASL-MRA and CTA better visualized the superior thyroid, lingual, facial, submental, transverse facial, and internal maxillary arteries (IMAs) better than TOF-MRA (p < 0.05). In addition, CTA was superior to ASL-MRA in visualizing only submental artery among these arteries (p = 0.0005). Alternatively, the ASL-MRA was superior for visualizing the middle meningeal artery (MMA) and IMA, compared to the CTA (p = 0.0001 and 0.0007, respectively). ASL-MRA was superior to the TOF-MRA and similar to the CTA in visualizing most of ECA branches. Furthermore, ASL-MRA can better visualize the periphery of MMA and IMA than CTA.
Development of a potency assay for CD34+ cell-based therapy for post-acute myocardial infarction
Authors: Aries Anne , Vignon Christine , Zanetti Céline , Goubaud Aurélien , Cormier Arthur , Diederichs Anne , Lahlil Rachid , Hénon Philippe , Garitaonandia Ibon 
Published: May 2023

Abstract
Background: Acute myocardial infarction (AMI) results from obstruction of a coronary artery and affects around 126 million individuals globally. We have shown in a small pilot study that intracardiac delivery of autologous CD34+ cells after myocardial infarction is safe and leads to long term improvement. The improvement was such that three patients initially recommended for early heart transplantation in this study, no longer required it years after the cell injection. After the successful results of this pilot study, we started a multicenter, randomized, controlled Phase I/IIb study in post-AMI to investigate the safety and efficacy of intramyocardial injection of expanded autologous CD34+ cells (ProtheraCytes®) (NCT02669810). While patient recruitment is ongoing, we have developed a potency assay for the batch release of ProtheraCytes®.
Methods: The proposed mechanisms of action by which ProtheraCytes® promote cardiac regeneration and revascularisation of damaged myocardial tissue is via the secretion of angiogenic factors and endothelial differentiation. We conducted a series of in vitro studies characterizing the growth factor secretion, exosome secretion, gene expression, cell surface markers, differentiation potential, and angiogenic potential of ProtheraCytes® clinical batches to develop a potency assay.
Results: Here we show that ProtheraCytes® secrete vascular endothelial growth factor (VEGF) and its concentration is significantly correlated with the number of CD34+ cells obtained after expansion (Pearson r = 0.7484; p-value = 0.0009). ProtheraCytes® also secrete exosomes containing proangiogenic miRNAs (126, 130a, 378, 26a), antiapoptotic miRNAs (21 and 146a), antifibrotic miRNAs (133a, 24, 29b, 132), and miRNAs promoting myocardial regeneration (199a and 590). We also show that ProtheraCytes® have in vitro angiogenic activity, express surface markers of endothelial and cardiomyocyte progenitor cells, and can differentiate in vitro into endothelial cells.
Conclusions: The potency assay should represent the product's mechanism of action, quantitatively measure the relevant biological product attribute, and have lot to lot consistency. Developing a potency assay is a required step before commencing the pivotal Phase 3 clinical studies. After the in vitro characterization of multiple ProtheraCytes® clinical batches, we established that measuring the concentration of VEGF provided the most practical, reliable, and consistent potency assay.
Carbon System State Determines Warming Potential of Emissions
Authors: Winkler Alexander J. , Myneni Ranga B. , Reimers Christian , Reichstein Markus , Brovkin Victor 
Published: May 2023

Abstract
Current strategies to hold surface warming below a certain level, e.g., 1.5 or 2 °C, advocate limiting total anthropogenic carbon emissions to ∼0.9 or ∼1.25 Eg C (1018 grams carbon), respectively1,2. These allowable emission budgets are based on a near-linear relationship between cumulative emissions and warming identified in various modeling efforts3–7. The IPCC assesses this near-linear relationship with high confidence in its Summary for Policymakers8 (§D1.1 and Figure SPM.10). Here we test this proportionality in specially designed simulations with a latest-generation Earth system model that includes an interactive carbon cycle with updated terrestrial ecosystem processes, and a suite of CMIP simulations. We find that atmospheric CO2 concentrations differ by ∼100 ppmv and surface warming by ∼0.3 °C (0.46 °C over land) for the same cumulated emissions (∼1.2 Eg C, 2 °C approximate carbon budget1). CO2 concentration and warming per 1 Eg of emitted carbon (Transient Climate Response to Cumulative Carbon Emissions6,9,10; TCRE) depend not just on total emissions, but also on the duration and time sequence of emissions, which heretofore have been ignored. The time dependency clearly arises due to lagged carbon sequestration processes on land, viz., ecological succession, land-cover, and demographic changes, etc., which are still poorly represented in most ESMs. This implies a temporally evolving state of the carbon system, but one which surprisingly apportions carbon into land and ocean sinks in a manner that is independent of the emission pathway. Therefore, even though TCRE differs for different pathways with the same total emissions, it is roughly constant when related to the state of the carbon system, i.e., the amount of carbon stored in surface sinks. Efforts to better understand the state of the carbon system with observations and refined models are needed to accurately project the impact of future emissions.
Remote monitoring of cancer patients with suspected Covid-19 using wearable sensors and regular mobile data networks; results of the RECAP feasibility study (NCT04397705)
Authors: Tivey Ann , Shotton Rohan , Taylor Sally , Oakley Rachel , Summerton Sara , Huddar Prerana , King Michael , Gibson Jane , Eastwood Charlotte , Cooksley Tim , Wong David , Radford John 
Published: May 2023

Abstract

Background: Cancer patients with Covid-19 causing less severe illness have been safely managed in an outpatient setting but they remain at risk of clinical deterioration. In this pilot study we assessed the feasibility of using wearable biosensors to monitor key physiological data in ambulatory, home based, cancer patients with Covid-19 infection. Two key components of feasibility were assessed: patient acceptability of the devices, and the reliability of transmitting the data in real-time to the treating hospital.
Methods: Ambulatory cancer patients with Covid-19 consented to wearing sensors monitoring heart rate, respiratory rate, and temperature, along with twice daily pulse oximetry measurements for up to three weeks. All physiologic data were transmitted from home to the treating hospital via a secure cellular data pathway. Data other than oxygen saturations was analysed retrospectively. Questionnaires and semi-structured interviews were completed by patients and thematic qualitative analysis performed.
Results: 8 patients (6 female, 2 male) with a median age of 62 years (range 40-73) were recruited. 2 patients completed 21 days on study. 2 patients were admitted to hospital due to decreased oxygen levels. 4 patients discontinued monitoring during the study period. Most participants liked the concept of active monitoring and felt confident about connecting the device and changing the sensors. Others found using the system more challenging. Data transmission was considered good or satisfactory in 5 participants.
Conclusions: This is the first study demonstrating the use of wearable sensors to monitor cancer patients at home with real-time transmission of data to the treating hospital. Participants were generally supportive of remote monitoring and most found the technology easy to use but some issues with usability were highlighted.
Registration:, The study was registered with Clinical Trials.gov on 21/05/2020, registration number: NCT04397705, https://clinicaltrials.gov/ct2/show/NCT04397705
Rhizosphere processes and nickel mobilization by the nickel hyperaccumulator Odontarrhena chalcidica
Authors: Risse Sören B.L. , Puschenreiter Markus , Tognacchini Alice 
Published: May 2023

Abstract
Background and aims Plant Ni uptake in aboveground biomass exceeding concentrations of 1000 µg g− 1 in dry weight is defined as Ni hyperaccumulation. Whether hyperaccumulators are capable of mobilizing larger Ni pools than non-accumulators is still debated and rhizosphere processes are still largely unknown. Aim of this study was to investigate rhizosphere processes and possible Ni mobilization by the Ni hyperaccumulator Odontarrhena chalcidica and to test Ni uptake in relation to a soil Ni gradient.Methods The Ni hyperaccumulator O. chalcidica was grown in a pot experiment on six soils showing a pseudo-total Ni and labile (DTPA-extractable) Ni gradient and on an additional soil showing high pseudo-total but low labile Ni. Soil pore water was sampled to monitor changes in soil solution ionome, pH, and dissolved organic carbon (DOC) along the experiment.Results Results showed that Ni and Fe concentrations, pH as well as DOC concentrations in pore water were significantly increased by O. chalcidica compared to unplanted soils. A positive correlation between Ni in shoots and pseudo-total concentrations and pH in soil was observed, although plant Ni concentrations did not clearly show the same linear pattern with soil available Ni.Conclusions This study shows a clear root-induced Ni and Fe mobilization in the rhizosphere of O. chalcidica and suggests a rhizosphere mechanism based on soil alkalinization and exudation of organic ligands. Furthermore, it was demonstrated that soil pH and pseudo-total Ni are better predictors of Ni plant uptake in O. chalcidica than labile soil Ni.
Reduced-order model of geometrically nonlinear flexible structures for fluid-structure interaction applications
Authors: Flament Theo , Placzek Antoine , Balmaseda Mikel , Tran Duc-Minh , Deü Jean-François 
Published: May 2023

Abstract
This paper deals with the numerical computation, via a reduced order models (ROM), of the vibrations of geometrically nonlinear structures triggered by the aeroelastic coupling with a fluid flow. The formulation of the ROM proposed in this paper is based on the projection on a basis of reduced dimension enhanced with dual modes. An explicit expression of the projected nonlinear forces is computed in a non-intrusive way based on the Implicit Condensation method. The resulting ROM is an improvement of the classical ICE method since the effects of membrane stretching are taken into account in the resolution of the dynamic equation of motion. Such a ROM aims to be adapted to follower aerodynamic unsteady loads. In this paper, the construction of the ROM is first detailed and validated under several load cases on a Euler-Bernoulli beam with von Kármán hypothesis. Then a fluid-structure partitioned coupling on a two-dimensional example involving vortex-induced vibrations is considered to demonstrate the capability of such ROM to replace a nonlinear FE solver. In this paper, the limitations of the ICE method are highlighted in the examples treated, while the ROM proposed overcomes such limitations and captures accurately the dynamics.
Comparison of Cradle Breastfeeding Position With Cross Cradle Breastfeeding Position in Term Newborns on Their Weight Pattern
Authors: Patel Shaily , Gaur Ajay 
Published: May 2023

Abstract
Objectives- Common breastfeeding (BF) positions are side-lying, cradle hold, cross cradle hold, football, laid-back. The literature to compare different BF positions in terms of maternal comfort is scarce. This study was conducted to see the effect of two BF positions, cradle and cross cradle, on weight pattern and urine frequency along with maternal comfort and latch score.Study Design- Randomised controlled trial is performed in 200 mother newborn dyads of gestational age between 36–40 weeks and weight loss pattern, maternal comfort and latch score compared between the two groups.Results- Study observed that the mean distribution of urinary flow was statistically highly significant (p value < 0.001) in cross cradle group. Cross cradle breastfeeding position showed higher maternal comfort score and latch score than cradle breastfeeding position.Conclusion- This study explores that cross cradle breastfeeding position is better in terms of maternal comfort and latch score than cradle breastfeeding position.
Artificial Intelligence-Assisted Ultrasound-Guided Focused Ultrasound Therapy
Authors: Sadeghi-Goughari Moslem , Rajabzadeh Hossein , Han Jeong-woo , Kwon Hyock-Ju 
Published: May 2023

Abstract
Focused ultrasound (FUS) therapy has emerged as a promising non-invasive solution for tumor ablation. Accurate monitoring and guidance of ultrasound energy is crucial for effective FUS treatment. Although ultrasound (US) imaging is a well-suited modality for FUS monitoring, US-guided FUS (USgFUS) faces challenges in achieving precise monitoring, leading to unpredictable ablation shapes and a lack of quantitative measurement. To address these challenges, we propose an artificial intelligence (AI)-assisted USgFUS framework that integrates an AI segmentation framework with ultrasound B-mode imaging for quantitative and real-time monitoring of FUS treatment. The AI framework can accurately identify and label ablated areas in the B-mode images captured during and after each FUS sonication procedure in real-time. To assess the feasibility of our proposed method, we developed an AI segmentation framework based on the Swin-Unet architecture and conducted an in vitro experimental study using a USgFUS setup and chicken breast tissue. The results indicated that the developed AI segmentation framework could immediately label the ablated tissue areas with \(93\%\) accuracy. These findings suggest that AI-assisted ultrasound monitoring can significantly improve the precision and accuracy of FUS treatments, suggesting a crucial advancement towards the development of more effective FUS treatment strategies.
Advancements in data extraction from natural history collections: automatic label extraction from specimen images using OCR and NER
Authors: Takano Atsuko , Cole Theodor C. H. , Konagai Hajime 
Published: May 2023

Abstract
Digital extraction of label data from natural history specimens along with more efficient procedures of data entry will become essential for documentation and global information availability in the near future. Herbarium collections have made great advances in this direction lately. In this study, using optical character recognition (OCR) and named entity recognition (NER) techniques, we have been able to almost automatically extract label data from herbarium specimen images. This system can be developed and run on a PC with standard specifications, and can also be applied to extracting label data from diverse kinds of natural history specimens, such as those in entomological collections. This system can facilitate the digitization and publication of natural history museum specimens around the world.
Effects of acute normovolemic hemodilution and allogeneic blood transfusion on postoperative complications of oral and maxillofacial flap reconstruction：a retrospective study
Authors: Li Wenhao , Li Xueer , Chen Yanhong , Li Yanling , Huang Zhiquan , Zhao Yili 
Published: May 2023

Abstract
Objective: Patients with oral and maxillofacial flap reconstruction often need clinical blood transfusion treatment due to massive blood loss. With the increasing limitations of allogeneic blood transfusion (ABT), acute normovolemic hemodilution (ANH) is being considered by doctors because of its advantages. By comparing the differences in blood index changes and postoperative complications of patients receiving ABT or ANH during reconstruction and repair of oral and maxillofacial tumor flaps, the purpose is to provide a reference for the clinical application of ANH.
Methods: The clinical data of 276 patients who received oral and maxillofacial flap reconstruction from September 25, 2017, to October 11, 2021, in the Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, were retrospectively analyzed. According to the mode of intraoperative blood transfusion, the patients were divided into the ABT group and the ANH group. The differences in blood indexes change and the incidence of postoperative complications between two groups were statistically analyzed.
Results: Of the 276 patients with ANH (124/276) and ABT (152/276), there were no differences in Δ Hb, Δ PT, Δ FIB (P>0.05), while Δ WBC, Δ PLT, Δ APTT and Δ D-dimer were significantly different (P<0.05). The data analysis showed that blood transfusion method wasn’t an independent factor of flap crisis (P>0.05), post-PT (OR=1.953, 95% CI: 1.232~3.095, P=0.004) was an independent factor of wound infection, BMI (OR=0.089, 95% CI: 0.017~0.462) and ASA grade (OR=6.373, 95% CI: 1.681~24.163) were independent factors of lung infection (P<0.05), and blood transfusion mode (B=0.310, β=0.360, P<0.001, ANH: ln hospital stay=2.20±0.37, ABT: ln hospital stay=2.54±0.42) was an independent factor affecting the length of hospital stay.
Conclusion: Both preoperative and postoperative blood transfusion Δ Hb, Δ PT, and Δ FIB were not different, and Δ WBC, Δ PLT, Δ APTT, and Δ D-dimer were different. There was no difference in the effects of the two blood transfusion methods on flap crisis, incision infection and lung infection after flap reconstruction, but ANH resulted in shortening by an average of 3.65 days of hospital stay compared to ABT.
Mediating Effect of Emotional and Behaviors Symptoms on the Relationship Between Parenting Behaviors and Sleep problems in Preschool Children
Authors: Ding Peng , Geng Menglong , Wu Xiaoyan , Tao Shuman , Tao Fangbiao 
Published: May 2023

Abstract
Background Sleep plays an important role in the growth and development of children, and are affected by many factors. There are few studies on parenting behaviors on preschool children's sleep problems. Understanding the influence of parents on children's sleep and potential factors are helpful to correct rearing strategies in time and better promote children's sleep health.Methods A population-based survey was conducted in 109 kindergartens in 11 cities in China. The social security consumption of children was evaluated through a questionnaire filled out by caregivers. Children's sleep problems were assessed by Children's Sleep Habits Questionnaire (CSHQ), the Parent Behavior Inventory (PBI) to evaluate the parenting behavior of preschool children, emotional and behaviors symptoms were assessed by Strengths and Difficulties Questionnaire (SDQ).Result Approximately 15.3% of the preschoolers reported sleep problems. The adjusted odds ratios (95% confidence interval) of having elevated total difficulties for sleep problems across the maternal hostile/coercive were 1.00, 1.38 (1.23, 1.54) and 1.86 (1.64, 2.10), respectively (P for trend < 0.001); The paternal hostile/coercive were 1.00, 1.17 (1.05, 1.30) and 1.59 (1.42, 1.79), respectively (P for trend < 0.001). The mediation analysis further revealed partial mediation effects of emotional and behavioral symptoms on the association between parenting behaviors and children sleep.Conclusions Parents' hostile/coercive parenting styles increase the risk of children's sleep problems, in which emotional and behavioral problems play a mediating role.
Instruments on symptom clusters in adult patients with hematological malignancies: A scoping review
Authors: Cheng Yin , Yan Jiali , Cheng Qiong , Jin Aiyun , Zhou Xiaoyu , Ding Shuyi 
Published: May 2023

Abstract
Background Patients diagnosed with hematological malignancies usually experience multiple symptoms. Assessment is the key point of symptom management. Therefore, an applicable and feasible instrument is vital.Methods A search was performed in these databases: PubMed, CINAHL complete, Web of Science, China HowNet Periodical Databank, WanFang Data. All identified citations were uploaded into EndNote X9 software and duplicates removed. Data extracted from the included studies were mapped. Results were reported in consistent with PRISMA-ScR guidelines.Results Of the 410 articles searched through, 58 met the inclusion criteria. A total of 13 instruments of symptom clusters-related hematological malignancy were identified, including seven generic instruments and six specific-disease instruments. Majority instruments did not have theoretical framework. Besides, emotional and cognitive components were absent from several instruments. There was a lack of the large-scale psychometric tests on most instruments.Conclusions It is vital to select an available instrument to measure symptom clusters. However, a paucity of the large-scale psychometric tests and theoretical framework create the challenges in choosing amongst the tools available.