bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft genome sequence of
Acetobacter persici
DS1, a strain for high-acidity vinegar fermentation
Authors: Tripathi Neha Mani , Singh Puneet Kumar , Yadav Asha , Sharma Krishna Kant , Jain Amit Kumar , Aseri Gajender Kumar , Sharma Deepansh , Singh Deepti 
Published: January 2025

ABSTRACT

The draft genome sequence of
Acetobacter persici
DS1 was isolated for high-acidity vinegar fermentation. The
A. persici
DS1 draft genome has a length of 8,793,442 bp, with 44.27% GC content, 3,053 proteins, 3,169 genes, and 15 genes that play an important role in acetic acid production.
Complete genome sequences of two actinomycetes containing abyssomicin-like gene clusters:
Kutzneria buriramensis
DSM 45791 and
Streptomyces
sp. NL15-2K
Authors: Williams Sam E. , Faurdal David , Jørgensen Tue S. , Back Catherine R. , Rooms Lynden D. , Hayes Martin A. , Willis Christine L. , Stach James E. M. , Curnow Paul , Race Paul R. 
Published: January 2025

ABSTRACT

Here, we report the resequencing, assembly, and annotation of two actinomycete genomes containing abyssomicin gene clusters.
Kutzneria buriramensis
DSM 45791 with a circular chromosome of 11,681,598 bp and 4 circular plasmids (14,175–207,548 bp) and
Streptomyces
sp. NL15-2K with a 12,368,159 bp linear genome and circular plasmid (11,584 bp).
Biofloc technology significantly reshapes water microbiome and improves survival rates in Japanese eel (
Anguilla japonica
)
Authors: Yang Jiho , Choi Hyunjun , Park Jun Seong , Cha Yehyeon , Hwang Ju-Ae , Oh Seung-Yoon 
Published: January 2025

ABSTRACT

Global aquaculture production faces the challenge of biologically cycling nitrogenous waste. Biofloc technology (BFT) systems offer the potential to reduce water consumption and eliminate waste products by using beneficial microorganisms to convert waste into usable nutrients or non-toxic molecules. Unlike flow-through systems (FTS), which depend on continuous water exchange and result in higher operational costs as well as limited microbiome stability, BFT operates without the need for constant water exchange. Instead, it leverages its inherent microbiome for nutrient recycling, which can help reduce associated costs. The Japanese eel (
Anguilla japonica
), consumed as a luxury food item in China, Japan, and South Korea, has seen little research related to BFT. In this study, we observed the survival and growth rates of eels treated with BFT over 8 weeks and monitored changes in the gut and water microbiome using 16S rRNA amplicon sequencing (metabarcoding) and quantitative PCR. Our research demonstrated that BFT treatment was more advantageous for eel growth compared with FTS in terms of eel survival rate, feed intake amount, and growth rate. Both treatments did not result in water quality conditions detrimental to the growth of eels. Alpha diversity was higher in BFT water microbiome than in FTS, but no differences were observed in the guts of eels between the two treatments. While community structures of water microbiome (taxonomic composition) differed between BFT and FTS, gut microbiome did not. To identify the operational taxonomic units (OTUs) that directly influence the feed consumption rate of eels, closely linked to their growth rate, we first determined the differentially abundant residents and then highlighted the most informative OTUs. In summary, our analysis identified microbial residents potentially associated with the higher growth rates observed in BFT-treated eels, highlighting the need for further functional studies.

IMPORTANCE

This study is significant as it addresses a critical gap in the application of Biofloc Technology (BFT) to Japanese eel (
Anguilla japonica
) aquaculture, a high-value species in East Asia. BFT’s potential to reduce water consumption and enhance growth through the use of beneficial microorganisms presents a sustainable solution to the challenges of nitrogenous waste management in global aquaculture. Our research provides the first comprehensive analysis of how BFT influences both the growth and microbiome composition of Japanese eels compared with traditional flow-through systems. By identifying specific microbial residents potentially linked to improved feed consumption and growth, this study opens new avenues for optimizing BFT in eel farming. The findings contribute to the broader understanding of microbial roles in aquaculture, highlighting the potential for BFT to support more sustainable and productive aquaculture practices.
Identifying specific subgroups of older patients at risk of repeated hospital readmissions and death after discharge in a prospective multicentre cohort in France
Authors: Visade Fabien , Babykina Genia , Carretero-Bravo Jesus , Deschasse Guillaume , Vandewalle Vincent , Beuscart Jean-Baptiste 
Published: January 2025

ObjectiveTo identify specific subgroups of older patients at risk of repeated hospital readmissions and death.DesignProspective, multicentre, DAMAGE (Patient Outcomes After Hospitalization in Acute Geriatric Unit) cohort of adults aged 75 and over, discharged from an acute geriatric unit (AGU) and followed up for 12 months.SettingSix recruiting hospital centres in the Hauts-de-France and Normandie regions of France.Main outcome measuresWe performed a latent class analysis to identify subgroups at risk of repeated hospital readmissions and death, followed by a logistic regression analysis to determine the characteristics associated with the identified subgroups.Results3081 patients were included (mean (SD) age: 86.4 (5.5)) and two subgroups were identified. In subgroup 1 (n=2169, 70.4%), only 619 (28.5%) patients were readmitted to hospital once during the follow-up, and 495 (22.5%) died. In subgroup 2 (n=912, 29.6%), all patients were readmitted to hospital at least twice, and 523 (57.8%) died. Subgroup 2 accounted for 29.6% of patients but 74.4% of hospital readmissions, with longer lengths of stay, and 51.6% of deaths. A multivariate logistic regression analysis identified only four characteristics weakly associated with the risk of being in subgroup 2 (at least one hospital admission in the 6 months preceding the index hospital admission, cancer, polymedication and weight changes (gain or loss) during the index hospital admission). The area under the receiver operating characteristic curve was 63%.ConclusionA latent class analysis showed that a population of older adults hospitalised in an AGU is divided into two subgroups with regard to the postdischarge outcomes: one subgroup (70% of the individuals) will have a low rate of hospital readmission and a moderate death rate, whereas the other will have a high rate of hospital readmission and a very high death rate. There is a need for predictive scores for both events, with a view to better targeting at-risk patients.Trial registration numberTrial registration number was approved by the local independent ethics committee (CPP Nord-Ouest IV, Lille, France) on 13 February 2015, with an amendment approved on 21 January 2016 (reference: IDRCB 2014 A01670 47, CNIL bxA15352514).
Exploring surgeon behavioural factors impacting the quality of care: protocol for a scoping review
Authors: Dhaliwal Bhavan , Goubran Doris , Fylyma Orest , Siwach Ina , Askin Nicole , Darbandi Maziar Fazel , Delisle Megan 
Published: January 2025

IntroductionModern surgery incorporates many aspects of care, including preoperative workup, surgical management and multidisciplinary collaboration to achieve favourable outcomes and high patient satisfaction. Current literature identifies variability in surgical practice and quality of care. The objective of this study is to fill the gap in the literature by identifying modifiable surgeon behavioural factors influencing the quality of care and to identify interventions and policies that modify these factors.Methods and analysisThis scoping review will be reported using Preferred Reporting Items for Systematic Reviews and Meta-analyses extension for scoping review guidelines. The protocol was drafted according to JBI Best Practice Guidance and Reporting Items for the Development of Scoping Review Protocols. A comprehensive search encompassing five databases (OVID Medline, OVID EMBASE, Cochrane Library (Central) and SCOPUS) was conducted. Search terms included ‘surgeons’, ‘surgeon characteristics’, ‘quality of care’ and ‘outcomes’ using AND, OR and ADJ2 Boolean operators. Studies describing interventions aimed at modifying behavioural surgeon factors influencing the quality of healthcare will be included. Studies describing institutional or system factors will be excluded. Searches were limited from 1 January 2000 to 1 January 2024 to capture modern surgery practices. Searches were peer reviewed as per Peer Review of Electronic Search Strategies 2015. Two independent reviewers will perform a title and abstract screening using DistillerSR and extract data on the participants, study methods, modifiable surgeon factors and interventions that modify these factors. The data will be qualitatively analysed using the COM-B Framework which describes how capability, motivation and opportunity constitute behaviour. We expect to compile a list of existing interventions aimed at modifying surgeon behaviours, analysing the success of existing interventions to improve patient outcomes and identifying modifiable surgeon factors that do not have interventions.Ethics and disseminationEthics approval and patient consent are not required. The results will be submitted to a peer-reviewed journal for publication.
Occupational noise exposure and maternal pregnancy complications: register-based cohort from urban areas in four Nordic countries
Authors: Pasanen Tytti P , Tiittanen Pekka , Roswall Nina , Persson Waye Kerstin , Selander Jenny , Sanchez Martinez Nestor , Sjöström Mattias , Vincens Natalia , Ögren Mikael , Aasvang Gunn Marit , Evandt Jorunn , Krog Norun Hjertager , Weyde Kjell Vegard , Khan Jibran , Gissler Mika , Lindstrøm Jonas Christoffer , Poulsen Aslak Harbo , Pershagen Göran , Sorensen Mette , Lanki Timo 
Published: January 2025

Objective
To assess the role of occupational noise exposure on pregnancy complications in urban Nordic populations.

Methods

A study population covering five metropolitan areas in Denmark, Finland, Norway and Sweden was generated using national birth registries linked with occupational and residential environmental exposures and sociodemographic variables. The data covered all pregnancies during 5–11 year periods in 2004‒2016, resulting in 373 184 pregnancies. Occupational noise exposure was based on a Swedish-developed job-exposure-matrix, containing measured A-weighted annual 8 hour noise levels (L
Aeq8h
), and linked with person-specific job-history. Outcomes included diagnosed gestational diabetes, gestational hypertension, mild pre-eclampsia and severe pre-eclampsia. The data were analysed with logistic regression models separately in each country, adjusting for maternal (age, parity, birth year, education and marital status) and residential environmental factors (low neighbourhood income, NO
2
and green and blue space). The results were combined by meta-analysis.

Results
Occupational noise exceeding 80 dB, compared with less than 70 dB, was associated with an increased odds of gestational diabetes in all countries, with a combined OR of 1.26 (95% CI 1.04 to 1.51), and mild pre-eclampsia in all countries except Finland, resulting in a combined OR of 1.22 (95% CI 0.99 to 1.51). Further adjustment by maternal body-mass index attenuated these associations. No association with gestational hypertension or severe pre-eclampsia was found.

Conclusions

Register data from four nationalities show that gestational diabetes and, tentatively, mild pre-eclampsia was increased among pregnant workers working in occupations where noise levels exceed 80 dB L
Aeq8h
but not in occupations with lower noise levels.
Mapping Trends Regarding the Cardiopulmonary Resuscitation: A Bibliometric Analysis of the Top 100 Cited Articles
Authors: Turan Ömer Faruk , Yardımcı Ali Sami , Yazla Merve , Işık Nurullah İshak , Koçak Abdullah Osman , Katipoğlu Burak 
Published: January 2025

Introduction: Despite significant medical and technological advancements, cardiac arrest remains a critical public health concern due to its persistently high mortality and morbidity rates. Consequently, research on cardiopulmonary resuscitation (CPR) is of significant importance.Materials and Methods: This study presents a bibliometric analysis of the 100 most‐cited articles in the field of CPR identified using the Scopus database without time restrictions. Analyses were conducted using VOSviewer and Bibliometrix software. Original research articles published in English were included.Results: The study revealed contributions from 798 distinct authors across 18 journals, with citation counts ranging from 218 least‐cited articles to 1194 most‐cited articles. The most recent article was published in 2020, and the most frequently used keywords were “CPR” and “heart arrest.” In addition, 20% of the analyzed articles were funded by military organizations.Conclusion: This analysis highlights the significant contributions of emergency medicine researchers and the notable development of CPR literature, particularly between the late 1990s and the early 2000s. While the most‐cited studies originated from journals with high H‐indices, the citation frequency of these articles showed a consistent decline over time. Furthermore, impactful articles in this field were predominantly published in general medical journals rather than in specialized emergency medicine journals. This study provides a foundational resource for researchers, especially early career academicians, seeking to engage in CPR‐related research.
Exploring Goal Attainment Scaling Among Occupational Therapy Students: A Retrospective Descriptive Analysis
Authors: Alduraidi Wafaa , Zhang E. , Foster Lauren , Mische-Lawson Lisa 
Published: January 2025

Purpose: This study explored the administration of goal attainment scaling (GAS) by entry‐level occupational therapy (OT) students, examining their competency following minimal training. While GAS is valuable for measuring progress toward individualized client goals, its implementation requires specific skills and training that may challenge entry‐level practitioners.Method: Using retrospective data from a study of sensory garments’ effects on children with autism spectrum disorder (ASD), we analyzed GAS administration by five entry‐level OT students. Students received both indirect training through their curriculum and 3 h of direct research‐specific preparation. Using a modified version of the GAS checklist, we evaluated students’ competency through video recordings of GAS administration sessions and written GAS.Results: Analysis revealed variable competency levels among students. While overall performance met 82.4% of checklist criteria, individual student competency varied considerably (54.5%–95.4%). Three of five students achieved the established 75% competency threshold. Students consistently met criteria for conceptual goal construction and timeframe specification but struggled with maintaining single dimensions of change and specifying observable behaviors.Conclusions: Entry‐level OT students demonstrated varying levels of competency in GAS administration following minimal training. Our findings suggest the need for more comprehensive training in specific aspects of GAS, particularly in scale construction and behavioral specification. These results have important implications for how OT programs prepare students to implement GAS in clinical practice.
Exosome Loaded in Microneedle Patch Ameliorates Renal Ischemia–Reperfusion Injury in a Mouse Model
Authors: Taghavi Samin , Keshtkar Somayeh , Abedanzadeh Mozhgan , Hashemi Mehrdad , Heidari Reza , Abolmaali Samira Sadat , Dara Mahintaj , Aghdaei Mahdokht Hossein , Sabegh Alireza , Azarpira Negar 
Published: January 2025

Introduction: Renal dysfunction due to ischemia–reperfusion injury (IRI) is a common problem after kidney transplantation. In recent years, studies on animal models have shown that exosomes derived from mesenchymal stem cells (MSC‐Exo) play an important role in treating acute kidney injury (AKI) and promoting tissue repair. The microneedle patch provides a noninvasive and targeted delivery system for exosomes. The purpose of this innovative approach is to combine MSC‐Exo with microneedle patches.Method: Exosomes were isolated from MSCs, characterized, and placed in the prepared microneedle patch. Then this construct was applied to the IRI mice model. After 7 days, the gene expression of miR‐34a and its targets B‐cell lymphoma‐2 (BCL‐2) and BCL‐2‐associated X (BAX), along with reactive oxygen species (ROS) and lipid peroxidation (LPO) production, was investigated. Additionally, renoprotection was evaluated for measuring blood urea nitrogen (BUN) and creatinine (Cr) and histopathology detection.Results: After using microneedle patches containing exosomes, the reduction of miR‐34a and BAX and enhancement of BCL‐2 were observed. Moreover, treatment by this construct decreased the production of ROS, LPO, BUN, and Cr and improved tissue damage.Conclusion: The use of a microneedle patch containing exosomes is a noninvasive method that enables the release of exosomes in a slow manner. In comparison to exosome injection alone, microneedle patch‐exosome treatment offers a longer and more targeted effect that improves renal IRI dysfunction and reduces tissue damage, potentially facilitating the clinical application of exosomes and improving graft survival.
Broad-Spectrum Efficacy of CEACAM6-Targeted Antibody–Drug Conjugate with BET Protein Degrader in Colorectal, Lung, and Breast Cancer Mouse Models
Authors: Kogai Hiroyuki , Tsukamoto Shuntaro , Koga Minaho , Miyano Masayuki , Akagi Tsuyoshi , Yamaguchi Atsumi , Mori Kiyoshi , Gotoh Kunihito , Nakazawa Youya 
Published: January 2025

Abstract
Despite remarkable advances in cancer treatment, most solid cancers remain difficult to cure. We recently developed an antibody–drug conjugate (ADC; 84-EBET) for pancreatic cancer by using the carcinoembryonic antigen–related cell adhesion molecule 6 (CEACAM6) antibody #84.7 and the bromodomain and extra-terminal (BET) protein degrader EBET. In this study, we showed the overexpression of CEACAM6 in colorectal, lung, and breast cancers and the broad-spectrum efficacy of 84-EBET in mouse models of these cancers. In vitro assays using cancer organoids and cell lines of colorectal, lung, and breast cancers revealed that 84-EBET was more potent than ADCs with known approved payloads—DXd, SN38, and monomethyl auristatin E—or standard chemotherapies. In mouse studies, a single injection of 84-EBET induced marked regression of colorectal-, lung-, and breast cancer patient–derived xenograft tumors and cell line–derived xenograft tumors. Moreover, in mouse syngeneic colorectal cancer, lung cancer, and breast cancer models resistant to PD-1 antibody, the combination of 84-EBET and PD-1 antibody induced complete regression of most tumors. Mechanistically, 84-EBET degraded bromodomain-containing protein 4 in both cancer and stromal cells via bystander efficacy. It decreased stromal inflammatory phenotypes and increased activated T-cell numbers in tumors. These results demonstrate that delivering BET protein degraders to tumors and their microenvironments via a CEACAM6-targeted ADC may be effective against a wide range of solid cancers.
Antropología de la Música en América Latina y el Caribe
Authors: Noleto Rafael da Silva , Pérez Guarnieri Augusto 
Published: January 2025

Os estudos que abordam os vínculos entre Antropologia, música, sons e silêncios vêm se aprofundando na medida em que a etnografia se voltou para populações ou grupos sociais para quem o aspecto sonoro é culturalmente determinante. A Antropologia contemporânea tem cada vez mais se voltado aos elementos sensoriais implicados tanto no fazer antropológico quanto nas diversas formas através das quais as pessoas vivenciam, compreendem, formulam e expressam suas práticas culturais. Neste sentido, a teoria antropológica e os textos etnográficos têm avançado no aprofundamento do debate sobre práticas encorporadas (embodied practices) que, consequentemente, geram conhecimentos encorporados, isto é, modos de compreensão do mundo que passam, necessariamente, pelo envolvimento do corpo em experiências performáticas e/ou sensoriais (sejam elas musicais, teatrais, de dança ou outras modalidades expressivas). Destacamos que o uso proposital do conceito “encorporado" (ao invés de “incorporado”) é uma referência explícita às contribuições teóricas de Diana Taylor (2013) e Richard Schechner (2013) para denotar uma imersão e utilização consciente do corpo na produção do conhecimento antropológico pelas vias do fazer artístico. Naquilo que particularmente nos interessa, consideramos que este (re)conhecimento encorporado do mundo advém dos fazeres e saberes musicais, assim como da relação que os sujeitos estabelecem, cotidianamente, com a dimensão sonora do mundo através da percepção e/ou produção do som no e pelo corpo.
Intra-voxel angular dispersion of fibers in corpus callosum decreases with healthy aging
Authors: Moss Hunter G. , Chen Andrew A. , Jensen Jens H. , Benitez Andreana 
Published: January 2025

Abstract
The goal of this study was to investigate how the angular dispersion of axonal fibers changes during the course of healthy aging. The angular dispersion was derived from the fiber orientation density function (fODF), which was estimated in vivo with a diffusion MRI technique called fiber ball imaging. Intra-voxel angular dispersion of axonal fibers within the corpus callosum at the midline up to the anterior tip of the frontal horn of the lateral ventricles was quantified for a cohort of 63 healthy older adults (ages 45 to 85 years). The splenium, body, and genu of the corpus callosum were examined separately, and fODFs within each of these regions were averaged across voxels to obtain three mean fODFs for each study participant. For all three regions, we found that the angular dispersion, as quantified by the full width of the mean fODF at half its maximum, decreases significantly with age. However, these decreases were not significantly different across the regions. In addition, the heights of the mean fODF peaks increase with age. This reduction in angular dispersion and increase in height imply axons with orientations deviating further from the fODF peak are more likely to be lost in the course of healthy aging. We propose that this is related to the known preferential loss of thinner myelinated axons with increasing age.
Interdependence patterns of multifrequency oscillations predict visuomotor behavior
Authors: Bahuguna Jyotika , Schwey Antoine , Battaglia Demian , Malfait Nicole 
Published: January 2025

Abstract
We show that sensorimotor behavior can be reliably predicted from single-trial EEG oscillations fluctuating in a coordinated manner across brain regions, frequency bands, and movement time epochs. We define high-dimensional oscillatory portraits to capture the interdependence between basic oscillatory elements, quantifying oscillations occurring in single trials at specific frequencies, locations, and time epochs. We find that the general structure of the element interdependence networks (effective connectivity) remains stable across task conditions, reflecting an intrinsic coordination architecture and responds to changes in task constraints by subtle but consistently distinct topological reorganizations. Trial categories are reliably and significantly better separated using oscillatory portraits than from the information contained in individual oscillatory elements, suggesting an interelement coordination-based encoding. Furthermore, single-trial oscillatory portrait fluctuations are predictive of fine trial-to-trial variations in movement kinematics. Remarkably, movement accuracy appears to be reflected in the capacity of the oscillatory coordination architecture to flexibly update as an effect of movement-error integration.