P0525 Genome-wild gene expression analysis and identification of Crohn’s disease from intestinal tuberculosis and primary intestinal lymphoma
Authors: Lin L , Wang Y , Zhao X 
Published: January 2025

Abstract

Background
Crohn’s disease(CD) is similar to intestinal tuberculosis(ITB) and primary intestinal lymphoma(PIL) in many aspects, and it is still difficult to distinguish CD from ITB and PIL by existing clinical methods. Genomics has been widely used in clinical and basic research, and DASL (cDNA-mediated annealing, selection, extension, and ligation) analysis is used to analyze transcripts extracted from formalin-fixed paraffin-embedded tissues.Therefore, the purpose of this study is to conduct genome-wide gene expression analysis of paraffin tissues by DASL technology, and screen out potential diagnostic genes, so as to assist the differential diagnosis between CD and ITB, PIL in clinical practice.

Methods
Paraffin samples from three diseases and healthy controls were collected, and gene expression analysis was performed by DASL technology.

Results
1. There are significant differences in gene expression in CD, ITB, PIL and healthy control tissues. A total of 72 paraffin samples from three diseases and healthy controls were collected.The expression of 19869 genes was detected by DASL technology, and the differentially expressed genes between diseases and diseases between healthy controls were obtained.
2. six potential diagnostic genes. Ephb3, CEL, SIGLEC10, CD70, CXCL13 and CHST10 were screened.
3. The expression of Ephb3 in intestinal mucosal tissues of CD was significantly upregulated and its function was explored. Another batch of paraffin samples from three diseases totaling 30 cases was collected, and immunohistochemistry showed that the expression of Ephb3 in the intestinal mucosal tissues of CD was significantly upregulated. According to GO enrichment analysis and existing research results, it is speculated that Ephb3 may be involved in the occurrence and development of CD by affecting the growth and development of the digestive system, cell adhesion, epithelial-mesenchymal transformation, and inflammation regulation.

Conclusion
1. There are significant differences in gene expression between CD and ITB, PIL. The differentially expressed genes have an impact on metabolic function, immune response, cell and biological adhesion, and discover pathways that may be related to the development of CD.
2. Ephb3 is significantly upregulated in the intestinal mucosa of patients with CD compared with the patients with ITB and PIL, and is expected to become an indicator of clinical differential diagnosis.
3. The data of this study show that Ephb3 is related to the development of the digestive system and nervous system, cell adhesion regulation, immune system regulation as well as other biological functions. This is highly consistent with existing findings, and we suspect that Ephb3 has the potential to influence the development of Crohn’s disease.

References
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