bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multisensory integration by polymodal sensory neurons dictates larval settlement in a brainless cnidarian larva
Authors: Birch Sydney , Plachetzki David 
Published: May 2023

AbstractMultisensory integration (MSI) combines information from more than one sensory modality to elicit behaviours distinct from unisensory behaviours. MSI is best understood in animals with complex brains and specialized centres for parsing different modes of sensory information, but dispersive larvae of sessile marine invertebrates utilize multimodal environmental sensory stimuli to base irreversible settlement decisions on, and most lack complex brains. Here, we examined the sensory determinants of settlement in actinula larvae of the hydrozoan Ectopleura crocea (Cnidaria), which possess a diffuse nerve net. A factorial settlement study revealed that photo‐, chemo‐ and mechanosensory cues each influenced the settlement response in a complex and hierarchical manner that was dependent on specific combinations of cues, an indication of MSI. Additionally, sensory gene expression over development peaked with developmental competence to settle, which in actinulae, requires cnidocyte discharge. Transcriptome analyses also highlighted several deep homological links between cnidarian and bilaterian mechano‐, chemo‐, and photosensory pathways. Fluorescent in situ hybridization studies of candidate transcripts suggested cellular partitioning of sensory function among the few cell types that comprise the actinula nervous system, where ubiquitous polymodal sensory neurons expressing putative chemo‐ and photosensitivity interface with mechanoreceptive cnidocytes. We propose a simple multisensory processing circuit, involving polymodal chemo/photosensory neurons and mechanoreceptive cnidocytes, that is sufficient to explain MSI in actinulae settlement. Our study demonstrates that MSI is not exclusive to complex brains, but likely predated and contextualized their evolution.
Genetic basis of ecologically relevant body shape variation among four genera of cichlid fishes
Authors: DeLorenzo Leah , Mathews Destiny , Brandon A. Allyson , Joglekar Mansi , Carmona Baez Aldo , Moore Emily C. , Ciccotto Patrick J. , Roberts Natalie B. , Roberts Reade B. , Powder Kara E. 
Published: May 2023

AbstractDivergence in body shape is one of the most widespread and repeated patterns of morphological variation in fishes and is associated with habitat specification and swimming mechanics. Such ecological diversification is the first stage of the explosive adaptive radiation of cichlid fishes in the East African Rift Lakes. We use two hybrid crosses of cichlids (Metriaclimasp.×Aulonocarasp. andLabidochromissp.×Labeotropheussp., >975 animals total) to determine the genetic basis of body shape diversification that is similar to benthic‐pelagic divergence across fishes. Using a series of both linear and geometric shape measurements, we identified 34 quantitative trait loci (QTL) that underlie various aspects of body shape variation. These QTL are spread throughout the genome, each explaining 3.2–8.6% of phenotypic variation, and are largely modular. Further, QTL are distinct both between these two crosses of Lake Malawi cichlids and compared to previously identified QTL for body shape in fishes such as sticklebacks. We find that body shape is controlled by many genes of small effect. In all, we find that convergent body shape phenotypes commonly observed across fish clades are most likely due to distinct genetic and molecular mechanisms.
The Iran‐Israel Conflict: An Ultra‐Ideological Explanation
Authors: Roomi Farshad 
Published: May 2023

AbstractIran's enmity with Israel is ideological in the first place and strategic in the second. Iran intends through anti‐Israel actions and messaging to internally mobilize its populace and, externally, to claim the leadership of the Muslim world and strike a balance against a regional nuclear power. This article uses a critique based on constructivism and realism to reveal how Iran's confrontation with Israel has evolved historically from “identity‐ideological” to “politico‐strategic.” I argue that Iran's adoption of this approach without taking its internal and external capacities into consideration has ironically bolstered the Israeli far right, increased global sympathies for Israel, escalated Iranophobia, aligned conservative Arab states with Israel, and marginalized the issue of Palestine. To preserve its national interests and regional security, Iran needs to overcome this politico‐historical stage and replace conflict with competition.
Evidential transformer for pavement distress segmentation
Authors: Tong Zheng , Ma Tao , Zhang Weiguang , Huyan Ju 
Published: May 2023

AbstractDistress segmentation assigns each pixel of a pavement image to one distress class or background, which provides a simplified representation for distress detection and measurement. Even though remarkably benefiting from deep learning, distress segmentation still faces the problems of poor calibration and multimodel fusion. This study has proposed a deep neural network by combining the Dempster–Shafer theory (DST) and a transformer network for pavement distress segmentation. The network, called the evidential segmentation transformer, uses its transformer backbone to obtain pixel‐wise features from input images. The features are then converted into pixel‐wise mass functions by a DST‐based evidence layer. The pixel‐wise masses are utilized for performing distress segmentation based on the pignistic criterion. The proposed network is iteratively trained by a new learning strategy, which represents uncertain information of ambiguous pixels by mass functions. In addition, an evidential fusion strategy is proposed to fuse heterogeneous transformers with different distress classes. Experiments using three public data sets (Pavementscape, Crack500, and CrackDataset) show that the proposed networks achieve state‐of‐the‐art accuracy and calibration on distress segmentation, which allows for measuring the distress shapes more accurately and stably. The proposed fusion strategy combines heterogeneous transformers while remaining a performance not less than those of the individual networks on their respective data sets. Thus, the fusion strategy makes it possible to use the existing networks to build a more general and accurate one for distress segmentation.
The mycorrhizal root‐shoot axis elicits Coffea arabica growth under low phosphate conditions
Authors: Chialva Matteo , Patono Davide Lucien , de Souza Leonardo Perez , Novero Mara , Vercellino Sara , Maghrebi Moez , Morgante Michele , Lovisolo Claudio , Vigani Gianpiero , Fernie Alisdair , Fiorilli Valentina , Lanfranco Luisa , Bonfante Paola 
Published: May 2023

Summary

Coffee is one of the most traded commodities world‐wide. As with 70% of land plants, coffee is associated with arbuscular mycorrhizal (AM) fungi, but the molecular bases of this interaction are unknown.

We studied the mycorrhizal phenotype of two commercially important Coffea arabica cultivars (‘Typica National’ and ‘Catimor Amarillo’), upon Funnelliformis mosseae colonisation grown under phosphorus limitation, using an integrated functional approach based on multi‐omics, physiology and biochemistry.

The two cultivars revealed a strong biomass increase upon mycorrhization, even at low level of fungal colonisation, improving photosynthetic efficiency and plant nutrition. The more important iconic markers of AM symbiosis were activated: We detected two gene copies of AM‐inducible phosphate (Pt4), ammonium (AM2) and nitrate (NPF4.5) transporters, which were identified as belonging to the C. arabica parental species (C. canephora and C. eugenioides) with both copies being upregulated. Transcriptomics data were confirmed by ions and metabolomics analyses, which highlighted an increased amount of glucose, fructose and flavonoid glycosides.

In conclusion, both coffee cultivars revealed a high responsiveness to the AM fungus along their root‐shoot axis, showing a clear‐cut re‐organisation of the major metabolic pathways, which involve nutrient acquisition, carbon fixation, and primary and secondary metabolism.
FC2 stabilizes POR and suppresses ALA formation in the tetrapyrrole biosynthesis pathway
Authors: Fan Tingting , Roling Lena , Hedtke Boris , Grimm Bernhard 
Published: May 2023

Summary
During photoperiodic growth, the light‐dependent nature of chlorophyll synthesis in angiosperms necessitates robust control of the production of 5‐aminolevulinic acid (ALA), the rate‐limiting step in the initial stage of tetrapyrrole biosynthesis (TBS). We are interested in dissecting the post‐translational control of this process, which suppresses ALA synthesis for chlorophyll synthesis in dark‐grown plants.
Using biochemical approaches for analysis of Arabidopsis wild‐type (WT) and mutant lines as well as complementation lines, we show that the heme‐synthesizing ferrochelatase 2 (FC2) interacts with protochlorophyllide oxidoreductase and the regulator FLU which both promote the feedback‐controlled suppression of ALA synthesis by inactivation of glutamyl‐tRNA reductase, thus preventing excessive accumulation of potentially deleterious tetrapyrrole intermediates.
Thereby, FC2 stabilizes POR by physical interaction. When the interaction between FC2 and POR is perturbed, suppression of ALA synthesis is attenuated and photoreactive protochlorophyllide accumulates. FC2 is anchored in the thylakoid membrane via its membrane‐spanning CAB (chlorophyll‐a‐binding) domain.
FC2 is one of the two isoforms of ferrochelatase catalyzing the last step of heme synthesis. Although FC2 belongs to the heme‐synthesizing branch of TBS, its interaction with POR potentiates the effects of the GluTR‐inactivation complex on the chlorophyll‐synthesizing branch and ensures reciprocal control of chlorophyll and heme synthesis.
Growth, physiology, and stomatal parameters of plant polyploids grown under ice age, present‐day, and futureCO2concentrations
Authors: Šmarda Petr , Klem Karel , Knápek Ondřej , Veselá Barbora , Veselá Kristýna , Holub Petr , Kuchař Vít , Šilerová Alexandra , Horová Lucie , Bureš Petr 
Published: May 2023

SummaryPolyploidy plays an important role in plant evolution, but knowledge of its eco‐physiological consequences, such as of the putatively enlarged stomata of polyploid plants, remains limited. Enlarged stomata should disadvantage polyploids at low CO2concentrations (namely during the Quaternary glacial periods) because larger stomata are viewed as less effective at CO2uptake.We observed the growth, physiology, and epidermal cell features of 15 diploids and their polyploid relatives cultivated under glacial, present‐day, and potential future atmospheric CO2concentrations (200, 400, and 800 ppm respectively).We demonstrated some well‐known polyploidy effects, such as faster growth and larger leaves, seeds, stomata, and other epidermal cells. The stomata of polyploids, however, tended to be more elongated than those of diploids, and contrary to common belief, they had no negative effect on the CO2uptake capacity of polyploids. Moreover, polyploids grew comparatively better than diploids even at low, glacial CO2concentrations. Higher polyploids with large genomes also showed increased operational stomatal conductance and consequently, a lower water‐use efficiency.Our results point to a possible decrease in growth superiority of polyploids over diploids in a current and future high CO2climatic scenarios, as well as the possible water and/or nutrient dependency of higher polyploids.
Satellite Rod Fixation around Rod‐fracture Area in Revision Surgery after Three‐column Osteotomy for Severe Kyphoscoliosis
Authors: Liu Dun , Shi Benlong , Liu Zhen , Sun Xu , Zhu Zezhang , Qiu Yong 
Published: May 2023

ObjectiveThree‐column osteotomy (3CO) is considered valuable and increasingly utilized in the surgical treatment of severe spine deformity while associated with high implant‐related complications and revision risks. This study aims to evaluate the feasibility and clinical outcomes of satellite rod fixation used around the rod‐fracture area in revision surgery due to rod fracture after 3CO.MethodsTwenty‐five patients applying satellite rod fixation in revision surgery from August 2012 to May 2016 were retrospectively reviewed as the SR group. Patients undergoing revision surgery with traditional strategy after 3CO due to rod‐fracture were selected as the TR group. Cobb angle, distance between C7 plumb line and center sacral vertical line (C7PL‐CSVL), global kyphosis (GK) and sagittal vertical axis (SVA) were assessed. Patients were required to fulfill the Scoliosis Research Society‐22 questionnaire (SRS‐22) at pre‐revision and the last follow‐up. The paired t test was used to analyze the difference among pre‐revision, post‐revision and last follow‐up.ResultsThere was no statistical difference in terms of age, gender, body mass index (BMI), fusion level at 1st surgery, and follow‐up period between SR and TR group (all P > 0.05). The operation time (1.5 ± 0.7 h vs 3.2 ± 0.9 h, P < 0.001) and intraoperative blood loss (178 ± 51 mL vs 324 ± 96 mL, P < 0.001) were significantly higher in the TR group. Patients in both groups obtained obvious deformity correction after revision surgery. For patients in SR group, the coronal Cobb angle significantly improved from 27.9 ± 21.5° at pre‐revision to 21.8 ± 16.6° at post‐revision (P = 0.034). The C7PL‐CSVL decreased from 22.6 ± 14.3 mm to 21.3 ± 10.9 mm (P = 0.719). Similarly, improvement was attained in post‐revision GK (25.8 ± 17.0° vs 20.2 ± 15.1°, P = 0.061). SVA was corrected from 35.6 ± 33.9 mm to 30.8 ± 24.3 mm after revision (P = 0.182). At the last follow‐up, no significant correction loss was observed in both coronal and sagittal parameters (all P > 0.05). All patients responded to the SRS‐22 questionnaire and all the domains showed improvements in different levels. As compared to the TR group, the SR group had significantly better pain and management satisfaction scores (all P < 0.05). Additionally, there was no reoccurrence of implant failure during follow‐up and all patients achieved solid bony fusion in SR group.ConclusionSatellite rod fixation around rod‐fracture area is indicated for patients in the requirement of revision surgeries due to rod fracture after 3CO. Compared with traditional revision strategies, revision surgery with satellite rods, if patients are selected adequately, is a simpler procedure with less intraoperative blood loss and shorter operating time.
Imaging fibrosis in pediatric kidney transplantation: A pilot study
Authors: Elsingergy Mohamed M. , Viteri Bernarda , Otero Hansel J. , Bhatti Tricia , Morales Tatiana , Roberts Timothy P. L. , Amaral Sandra , Hartung Erum , Serai Suraj D. 
Published: May 2023

AbstractBackgroundNoninvasive alternatives to biopsy for assessment of interstitial fibrosis and tubular atrophy (IFTA), the major determinant of kidney transplant failure, remain profoundly limited. Elastography is a noninvasive technique that propagates shear waves across tissues to measure their stiffness. We aimed to test utility of elastography for early detection of IFTA in pediatric kidney allografts.MethodsWe compared ultrasound (USE) and MR elastography (MRE) stiffness measurements, performed on pediatric transplant recipients referred for clinically indicated biopsies, and healthy controls.ResultsTen transplant recipients (median age 16 years) and eight controls (median age 16.5 years) were enrolled. Three transplant recipients had “stable” allografts and seven had Banff Grade 1 IFTA. Median time from transplantation to biopsy was 12 months. Mean estimated glomerular filtration rate was 61.5 mL/min/1.73m2 by creatinine‐cystatin‐C CKiD equation at time of biopsy. Mean stiffness, calculated through one‐way ANOVA, was higher for IFTA allografts (23.4 kPa USE/5.6 kPa MRE) than stable allografts (13.7 kPa USE/4.4 kPa MRE) and controls (9.1 kPa USE/3.6 kPa MRE). Pearson's coefficient between USE and MRE stiffness values was strong (r = .97). AUC for fibrosis prediction in transplanted kidneys was high for both modalities (0.91 USE and 0.89 MRE), although statistically nonsignificant (p > .05). Stiffness cut‐off values for USE and MRE were 13.8 kPa and 4.6 kPa, respectively. Both values yielded a sensitivity of 100% but USE specificity (72%) was slightly higher than MRE (67%).ConclusionElastography shows potential for detection of low‐grade IFTA in allografts although a larger sample is imperative for clinical validation.
About time: Temporal control and illegality in Nashville, Tennessee
Authors: Flores Andrea 
Published: May 2023

AbstractThis article examines how time creates immigrant il/legality. It centers on a young, undocumented immigrant who was stopped by police following a traffic violation and held in custody pending potential deportation. However, he was ultimately released due to previously filed legal claims. Through the case, I demonstrate how he, his lawyer, the police, and his everyday contacts advance or attempt to thwart his claims to legality through advancing different, and often moralized, notions of time. Specifically, I show how these actors tie legality to specific points in time, or periods of time, including age. A temporal focus reveals the flexibility of il/legality and how non‐state actors—including undocumented immigrants themselves—wrest control over their lives and participate in defining the legal. The limits of temporal control, evidenced in the arbitrary nature of timing itself, also demonstrate the ultimate serendipity of legality.
Budding of ascospores in Colletotrichum lindemuthianum
Authors: Martins Ana Carolina Faria , Dias Mariana Andrade , Pereira Fernanda Aparecida Castro , Mendes‐Costa Maria Cristina , de Souza Elaine Aparecida 
Published: May 2023

AbstractCommon bean anthracnose is one of the main fungal diseases that affect the crop. The disease is caused by the fungus Colletotrichum lindemuthianum, anamorph, which has wide variability as well as its teleomorph. Sexual reproduction is the main mechanism of increasing genetic variability in fungi, therefore the study of morphological characteristics and their sexual structures are important. Homothallic strains of the pathogen have shown the production of new ascospores via mitosis after the sexual cycle. As a result of each mitotic division there are two ascospores with amygdaliform and/or allantoid morphology. In this work the characterization of this phenomenon is described as budding. This is the first report on the occurrence of ascospore budding in the genus Colletotrichum. The homothallic strain UFLA84‐1 was used. Analyses of light microscopy, fluorescence and high‐resolution scanning electron microscopy were used for the description of the budding process. Cytological analyses were carried out to estimate the dimension of ascospores, budding percentage and colony type. The budding cycle is described in six stages, and the complete process develops after the release of all ascospores from an ascus. The ascospores from this process can present both morphologies and bud growth can be apical or lateral. Sexual reproduction is a mechanism of genetic recombination and the budding process increases the number of ascospores generated by meiosis and may therefore represent an adaptive advantage of the pathogen.
Rules as policy data? Measuring and linking policy substance and legislative context
Authors: Hurka Steffen , Knill Christoph , Steinebach Yves 
Published: May 2023

AbstractThere is growing scholarly interest in analyzing changes in policies, laws, and regulations. Some concepts depart from the goal of identifying changes in policy substance. Other contributions have concentrated on the structural characteristics of laws and regulations containing these substantive changes. Extracting measures of policy substance from legislative texts is a challenging and time‐consuming endeavor as it requires the manual assessment and coding of legal acts. The assessment of the structural characteristics of laws and regulations, by contrast, can be done applying automated natural language processing. An important critical question is, thus, whether we can combine these approaches and simplify the information extraction by inferring changes in the policy substance from the legislative context in which these changes are embedded. Examining more than 100 legal acts in the area of EU environmental and climate policy, we find that the measures capturing policy substance and the structural characteristics of legal acts context are not systematically linked. In other words: changes in the structural features of legal acts cannot be used as an approximation for changes in policy substance. We conclude by sketching out a research agenda when (and when not) to use the different concepts and related measurements.
Linguistic Relativity in Cross‐Cultural Context: Converging Evidence From Neuroanthropology
Authors: Downey Greg , Gillett Alexander J. 
Published: May 2023

AbstractKemmerer's discussion of links between semantic typology and embodied cognition is welcome, especially his survey of available evidence. Focusing on mechanisms of embodied enculturation, however, we must understand that language is just one part of developmental assemblies that shape cognition, alongside other cultural elements such as sensory learning, behavior patterns, social interactions, and emotional experience. We believe that a source of this problem is an obsolete definition of “culture” as shared mental information that is inconsistent with models of embodied cognition and yet pervasive in human and cognitive sciences. We point to microethnographies of cognitive ecologies as a tractable remedy.
Different photorespiratory mechanisms in conifer leaves, where peroxisomes have intrinsically low catalase activity
Authors: Miyazawa Shin‐Ichi , Ujino‐Ihara Tokuko , Miyama Takafumi , Tahara Ko , Tobita Hiroyuki , Suzuki Yuji , Nishiguchi Mitsuru 
Published: May 2023

SUMMARYPhotorespiration is an essential metabolic mechanism associated with photosynthesis; however, little is known about the photorespiratory pathway of conifer gymnosperms. Metabolite analyses of the leaves of 27 tree species showed that the mean glycerate content in conifer leaves was lower than that in angiosperm leaves. We performed experiments where [13C]‐serine was fed to detached shoots of a conifer (Cryptomeria japonica), via the transpiration stream, and compared the labeling patterns of photorespiratory metabolites with those of an angiosperm tree (Populus nigra), because glycerate is produced from serine via hydroxypyruvate in peroxisomes. In P. nigra, hydroxypyruvate, glycerate and glycine were labeled with 13C, whereas in C. japonica, glycolate and a non‐canonical photorespiratory metabolite, formate, were also labeled, suggesting that an H2O2‐mediated non‐enzymatic decarboxylation (NED) reaction occurs in C. japonica. We analyzed changes in the metabolite contents of leaves kept in the dark and leaves exposed to illuminated photorespiration‐promoting conditions: a positive relationship between formate and serine levels in C. japonica implied that the active C1‐metabolism pathway synthesizes serine from formate. Leaf gas exchange analyses revealed that CO2 produced through NED was recaptured by chloroplasts. Database analysis of the peroxisomal targeting signal motifs of an H2O2‐scavenging enzyme, catalase, derived from various species, including nine coniferous species, as well as analyses of peroxisomal fractions isolated from C. japonica and P. nigra leaves indicated that conifer peroxisomes had less catalase activity. These results suggest that NED and the subsequent C1 metabolism are involved in the photorespiratory pathway of conifer leaves, where peroxisomes have intrinsically low catalase activity.