bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fault diagnosis method of rotating machinery based on improved multiscale attention entropy and random forests
Authors: Chen Fei , Zhang Liyao , Liu Wenshen , Zhang Tingting , Zhao Zhigao , Wang Weiyu , Chen Diyi , Wang Bin 
Published: January 2023

Abstract
In order to precisely diagnose the fault type of rotating machinery, a fault diagnosis method for rotating machinery based on improved multiscale attention entropy and random forests is proposed in this study. Firstly, a nonlinear dynamics technique without hyperparameters namely multiscale attention entropy is proposed for measuring signal complexity by extending attention entropy to multiple time scales. Secondly, aiming at the insufficient coarse graining of multiscale attention entropy, composite multiscale attention entropy is exploited to extraction the features of rotating machinery faults. Then, t-distributed stochastic neighbor embedding is used to overcome the feature redundancy problem by reducing the dimension of the extracted features. Finally, the reduced-dimensional features are inputted into the random forests model to complete fault pattern recognition of rotating machinery. The results of the experiment indicate that the proposed method achieves 98.216%and 98.506% diagnosis rates on two different fault datasets respectively, showing an extremely competitive advantage in comparison with conventional diagnosis models. Meanwhile, the proposed method is adopted to the actual hydropower unit without misjudgment, which verifies its strong adaptability. The research proposes a novel method for detecting faults in rotating machinery such as hydropower units.
Tackling fungal diversity in lichen symbioses: molecular and morphological data recognize new lineages in Chaetothyriales (Eurotiomycetes, Ascomycota)
Authors: Cometto Agnese , Leavitt Steven , Grube Martin , de Hoog Sybren , MUGGIA LUCIA 
Published: January 2023

Abstract
Lichens have been reappraised as self-sustaining and long-living ecosystems in which a multiplicity of microorganisms are housed, in addition to the main symbiotic partners. Lichen-associated microfungi can frequently occur cryptically, and their species diversity has recently been more fully elucidated by DNA metabarcoding studies and culture isolations. These lichen-associated fungi represent a wide array of major lineages in Ascomycetes and Basidiomycetes, including both filamentous and yeast species. Thanks to culture isolations, the morphology of a subset of the lichen-associated microfungal diversity has been studied. Metabarcoding analyses have shown high diversity of ascomycetous lichen-associated fungi in the two cosmopolitan rock-inhabiting lichens – Rhizoplaca melanophthalma and Tephromela atra – and many of these taxa were successfully isolated in culture. Based on DNA sequence data and morphological analyses, two new lineages within Chaetothyriales are here recognized. Both occur in lichens from dry habitats and are described here as the new species Cladophialophora endolichena Cometto, de Hoog, Muggia and Paracaldophialophora lichenicola Cometto, de Hoog, Muggia. Other strains are placed in Pleostigmataceae, Trichomeriaceae, Pleosporales, Mycosphaerellales, Coniochaetales and Hypocreales, further filling gaps of knowledge of the high fungal diversity residing in lichen thalli.
Minichromosome maintenance 6 protects against renal fibrogenesis by regulating DUSP6-mediated ERK/GSK-3β/Snail1 signaling
Authors: Huang Jing , Xu Zhi-Feng , Liu Feng , Song An-Ni , Su Hua , Zhang Chun 
Published: January 2023

Abstract
Background

Renal fibrosis is a major determinant of chronic kidney disease (CKD) and an inevitable outcome in all types of progressive CKD. Minichromosome maintenance 6 (MCM6) promotes the migration and invasive ability of tumor cells by regulating the epithelial-mesenchymal transition (EMT) cascade, but its exact biological function in kidney diseases remains unclear. In this study, we aim to explore the role and potential mechanism of MCM6 in renal fibrosis.

Methods

Two unrelated in vivo fibrotic models including unilateral ureteral obstruction (UUO) and unilateral ischemia-reperfusion injury (UIRI), and two in vitro tubular epithelial cells (TECs) injury models including TGF-β1-induced injury and hypoxia/reoxygenation-induced injury, were established to detect the expression of MCM6 in fibrotic models. And two adeno-associated viruses harboring MCM6 were delivered into the mice kidney via intraparenchymal injection to knockdown or overexpress the expression of MCM6 in renal tubules prior to the establishment of the UUO or UIRI model in order to further explore the specific role of MCM6 in renal fibrosis. Hematoxylin and eosin, Masson’s trichrome, immunohistochemical and immunofluorescence staining, western blotting assay, and qRT-PCR were performed to identify the effect of MCM6 on tubular injury, partial EMT, and interstitial fibrosis.

Results

MCM6 was significantly upregulated in TECs during progressive renal fibrosis including in vivo fibrotic models and in vitro injury stimulations. Conditional gene silencing of MCM6 aggravated partial EMT, extracellular matrix accumulation, and myofibroblast activation in UUO- or UIRI-induced renal fibrosis. And overexpression of MCM6 promoted the recovery of E-cadherin and suppressed the deposition of fibrotic markers, thereby retarding UUO- or UIRI-induced renal fibrosis. Mechanistically, activation of ERK/GSK-3β/Snail1 signaling was associated with MCM6-induced partial EMT. Additionally, DUSP6 expression substantially decreased in fibrotic kidneys and that it could be involved in MCM6-induced renal fibrosis by regulating ERK phosphorylation.

Conclusion

Our results are the first to identify the upregulation of MCM6 in fibrotic kidneys and further provide direct evidence that MCM6 play an important role in maintaining the tubular epithelial phenotype and protecting against renal fibrosis. MCM6 may be a useful biomarker for renal fibrosis and a potential anti-fibrotic therapeutic target for patients with CKD.
An Improved Biodegradable Microwave Absorber Based on Peanut Shell Mixed with Metal Free Carbon Nanotube for Ku-Band
Authors: Gupta Hitender , Khanna Rajesh , Rai Mayank Kumar 
Published: January 2023

Abstract
Agriculture waste has been used to develop environment friendly microwave and pyramidal absorbers, based on their dielectric constant value, for anechoic chambers applications. This paper conferred the dielectric parameters and microwave absorption profile based on two samples, one of peanut shell (PS) and second of PS mixed with 1 wt% metal free multi-walled carbon nanotubes (MF-MWCNT) composite (PSCNT)for frequency range 12 GHz to 18 GHz (Ku-band).The measured reflectivity for sample 2 of PSCNT is found to be -16.92 dB and − 28.36 dB as compared to sample1 of PS as -8.35dB and − 12.46 dB at13.3 GHz and 15.76 GHz, respectively. The finding shows the PSCNT composite has improved microwave absorption and good absorptivity in the Ku-band. The results indicate that it can replace the existing agriculture based microwave absorber.
Safety analysis of new medications in clinical trials: A simulation study to assess the differences between cause-specific and subdistribution frameworks in the presence of competing events
Authors: Genet Astrid , Bogner Kathrin , Goertz Ralf , Böhme Sarah , Leverkus Friedhelm 
Published: January 2023

Abstract

Safety is an essential part of the evaluation of new medications and competing risks that occur in most clinical trials are a well identified challenge in the analysis of adverse events. Two statistical frameworks exist to consider competing risks: the cause-specific and the subdistribution framework. To date, the application of the cause-specific framework is the standard practice in safety analyses. Here we analyze how the safety analysis results of new medications would be affected if instead of the cause-specific the subdistribution framework was chosen. We conducted a simulation study with 600 participants, equally allocated to verum and control groups and a 30 months follow-up period. Simulated trials were analyzed for safety in a competing risk (death) setting using both the cause-specific and subdistribution frameworks. Results show that comparing safety profiles in a subdistribution setting is always more pessimistic than in a cause-specific setting. For the group with the longest survival and a safety advantage in a cause-specific setting, the advantage either disappeared or a disadvantage was found in the subdistribution analysis setting. These observations are not contradictory but show different perspectives. To evaluate the safety of a new medication over its comparator, one needs to understand the origin of both the risks and the benefits associated with each therapy. These requirements are best met with a cause-specific framework. The subdistribution framework seems better suited for clinical prediction, and therefore more relevant for providers or payers, for example.
Mitochondrial metabolism as a potential novel therapeutic target for lung adenocarcinoma
Authors: Fujiwara Makoto , Mimae Takahiro , Kushitani Kei , Tsubokawa Norifumi , Miyata Yoshihiro , Takeshima Yukio , Okada Morihito 
Published: January 2023

Abstract
Background
Lung adenocarcinoma (LUAD) is the most common histological type of lung cancer and one of the leading causes of cancer-related deaths worldwide. The prognosis for LUAD patients remains unsatisfactory. To improve the prognosis of LUAD patients, identifying novel therapeutic targets is necessary. Oxidative phosphorylation (OXPHOS) is involved in the progression and metastasis of several cancers. This study evaluated the role of OXPHOS in LUAD and determined the potential for LUAD cell growth suppression by inhibiting OXPHOS metabolism.
Methods
Gene expression profiles, clinicopathological characteristics, and prognosis of lung cancer patients were evaluated using the OXPHOS or glycolysis-related RNA-seq data extracted from The Cancer Genome Atlas (TCGA) dataset. PPARγ expression, a representative OXPHOS molecule, was investigated using TCGA dataset and immunohistochemistry of surgically resected LUAD specimens. Expression of glycolysis-related molecules HIF1a, and LDH and OXPHOS-related molecules UCP2 and PPARγ and the effects of OXPHOS inhibitors oligomycin and metformin on cell growth were examined in human LUAD cell lines.
Results
High expression of OXPHOS-related genes was associated with worse prognosis and lymph node metastasis than that observed with low expression of OXPHOS-related genes (p = 0.07 and p < 0.01, respectively). In LUAD patients (n = 500), high PPARγ expression (n = 109) was associated with significantly worse prognosis than that seen with low PPARγ expression (n = 391) (5-year OS, high 34% vs. low 42%) (p = 0.01). PPARγ, detected in the invasive component of LUAD, was expressed only in the peripheral area. A549, HTB181, and H322 cells were classified as OXPHOS-high type, and H596 cells were classified as OXPHOS-low type, based on OXPHOS and glycolysis-related gene expression. Oligomycin treatment inhibited the proliferation of these OXPHOS-high-type cell lines (ratio of oligomycin 1.0 µM to control; A549:0.72, HTB181:0.69, H322:0.77, p < 0.01, respectively) but not of the OXPHOS-low expression type cell lines.
Conclusions
LUADs can be classified as high and low OXPHOS types, with heterogeneity in individual tumors. Inhibition of OXPHOS metabolism may represent a novel therapeutic strategy for LUAD patients with a high expression of OXPHOS-related genes.
Identification, Isolation, and Structural Characterization of Novel Forced Degradation Products of Ertugliflozin using Hyphenated Analytical Techniques HRMS, 2D-NMR, UPLC-MS, PREP-HPLC, and FT-IR
Authors: Salakolusu Suresh , Ganapavarapu Veera Raghava Sharma , Katari Naresh Kumar , Kaliyaperumal Muralidharan , Puppala Umamaheshwar , Ranga Mahesh , Jonnalagadda Sreekantha Babu 
Published: January 2023

Abstract
The research elucidates the stress degradation behavior of Ertugliflozin, which is used for the treatment of type-2 diabetics. The degradation was conducted as per ICH guidelines and Ertugliflozin is relatively stable in thermal, photolytic, and alkaline hydrolysis conditions; however, considerable degradation was detected in acid hydrolysis and oxidative hydrolysis. Degradation products were identified by ultra-high-performance liquid chromatography-mass spectrometry, isolated by semi-preparative high-performance liquid chromatography, and structural characterization using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Total four degradation products were identified and isolated in acid degradation, which are degradation products 1, 2, 3, and 4. Whereas in oxidative conditions, degradation product 5 was identified. All the five degradation products formed are novel, which was not reported earlier. This is the first time documented complete structural characterization of all five degradation products by using a hyphenated analytical technique. High-resolution mass, and nuclear magnetic resonance spectroscopy were used in the present study to get concrete confirmation of degradation products structures. The current method is also used to identify degradation products with shorter runtime in the future.
A Retrospective Cohort Review of the Health Disparities in the Older Adult Population with the Recent Surge in Telemedicine Use
Authors: Szerszen Anita , Kogan Yulia , Valsechi-Diaz Jonathan , Demissie Seleshi , Chacko Jincy , Alviar Kevin , Vig Raman , Sahni Puneet , Romm Yuval , Mishra Simita , Oppenheim Michael , Burns Edith 
Published: January 2023

Abstract
Background
Evaluate telemedicine uptake (audio-video and audio-only communications) and document sociodemographic differences amongst older adults during 2020.
Methods
Retrospective review of electronic medical records of patients 65 + years seen in three geriatric practices the New York metropolitan area January-November 2020. Multivariable regression evaluated association of demographic, socioeconomic factors and visit type.
Results
About a third of patients engaged in telemedicine visits. Telemedicine visits represented 80% of all visits in April dropping to an average of 11.8% by November. Use was similar across age groups, gender, race and insurance payer status between telemedicine versus in-person encounters. Patients with more comorbidities were more likely to use telemedicine. 47.5% of patients who engaged in video visits had another person/family member present during an encounter.
Conclusions
Telemedicine augmented access to health care across sociodemographic characteristics in this geriatric population. It may serve as a tool to reduce enduring health care disparities beyond the pandemic.
Delineation of shallow volcanic structures from audio-frequency magnetotelluirc data beneath Ulleung Island, East Sea (Sea of Japan)
Authors: Hong Jinpyo , Kim Kiyeon , Oh Seokhoon , Kwon Hyoung-Seok , Choi Sung Hi , Park Sun-Cheon 
Published: January 2023

Abstract
As volcanoes are closely related to the living environment of humans, in particular via natural hazards and geothermal energy, scientific studies on volcanic edifice structures are required. Ulleung Island is a quaternary volcanic island located in the mid-western East Sea (Sea of Japan). In this study, we conducted an audio-frequency magnetotelluric (AMT) survey to image the substructure of the Ulleung volcanic edifice. In addition, the electrical structure was also interpreted from the geothermal system perspective, the geochemical compositions of volcanic rocks, and the possibility of the presence of a magma chamber. AMT data were obtained from 25 stations and processed using remote reference techniques. Then, the three-dimensional (3-D) approach was reasonably adopted according to dimensionality analysis. Before conducting the 3-D inversion, the effects on topography and ocean were analyzed using a simplified 3-D synthetic model, because Ulleung Island is surrounded by the sea and the topography is undulating. Most AMT stations on Ulleung Island are distorted by topographical and oceanic effects; in particular, oceanic effects are significant at frequencies lower than 10 Hz. The 3-D inversion was conducted with full impedance components and vertical magnetic transfer functions at a frequency range of 10,000–1 Hz. The results showed that the Ulleung volcanic edifice is characterized by three layers of electrical structures as follows: the near-surface, a conductive layer, and a layer with relatively constant resistivity. In addition, the eruption pathway of volcanic materials that formed the Nari caldera and Albong was estimated. Considering the high geothermal gradient on Ulleung Island, we suggest that the conductive layer of the volcanic edifice is due to hydrothermal alteration of basaltic rocks, with a potential heat source underneath. Finally, based on the geochemical characteristics of the Ulleung volcanic rocks, the possibility of heat transfer from a trachytic magma reservoir within the shallow crustal depths is suggested. In summary, this study presents a geological interpretation of the substructure of Ulleung Island and the possibility of an active magma reservoir as a potential heat source for the volcanic edifice.
A TRPV4 mutation caused Charcot-Marie-Tooth disease type 2C with scapuloperoneal muscular atrophy overlap syndrome and scapuloperoneal spinal muscular atrophy in one family: a case report and literature review
Authors: Chen Haofeng , Sun Chong , Zheng Yongsheng , Yin Junxiong , Gao Mingshi , Zhao Chongbo , Lin Jie 
Published: January 2023

Abstract
Background
Charcot-Marie-Tooth disease 2C (CMT2C) and scapuloperoneal spinal muscular atrophy (SPSMA) are different clinical phenotypes of TRPV4 gene mutation. The mutation of p.Arg316Cys has been previously found to cause CMT2C and SPSMA separately.
Case presentation
Here, we reported a Chinese family harboring the same p.Arg316Cys variant but with overlap syndrome and different clinical manifestations. A 58-year-old man presented with severe scapula muscle atrophy as hanger-like shoulders. Distinct muscle atrophy also presented in his four limbs especially in the lower limbs. Sural nerve biopsy of the father indicated severe loss of myelinated nerve fibers with scattered regenerating clusters and pseudo-onion bulbs. Nerve conduction study indicated axon damage in both motor and sensory nerves. Sensory nerve action potentials could not be evocated in bilateral sural or superficial peroneal nerves. He was diagnosed with Charcot-Marie-Tooth disease type 2C and scapuloperoneal muscular atrophy overlap syndrome. While his 27-year-old son was born with club foot and clinodactyly. Electromyogram examination indicated chronic neurogenic changes and anterior horn cells involvement. He complained of no obvious weakness or sensory symptoms currently. Early SPSMA could be considered for him.
Conclusion
A literature review of the clinical characteristics in CMT2C and SPSMA patients with TRPV4 gene mutation suggested that our case was distinct for his overlap syndrome. Altogether, this case broadened the phenotype spectrum and supplied the nerve biopsy pathological characters of TRPV4 gene mutation related neuropathy.
Personalising genetic counselling (POETIC) trial: A hybrid type II effectiveness-implementation randomised clinical trial of a patient screening tool to improve patient empowerment after cancer genetic counselling
Authors: Forrest Laura Elenor , Tutty Erin , De Silva Anurika P , Petelin Lara , Ruscigno Amy , Purvis Rebecca , Monohan Katrina , Kentwell Maira , Sexton Adrienne , Stafford Lesley , James Paul Andrew 
Published: January 2023

Abstract
Background: Genetic counselling aims to identify, and address, patient needs while facilitating informed decision-making about genetic testing and promoting empowerment and adaptation to genetic information. Increasing demand for cancer genetic testing and genetic counsellor workforce capacity limitations may impact the quality of genetic counselling provided. The use of a validated genetic-specific screening tool, the Genetic Psychosocial Risk Instrument (GPRI), may facilitate patient-centred genetic counselling. The aim of this study is to assess the effectiveness and implementation of using the GPRI in improving patient outcomes after genetic counselling and testing for an inherited cancer predisposition.
Methods: The PersOnalising gEneTIc Counselling (POETIC) trial is a hybrid type 2 effectiveness-implementation trial using a randomised control trial to assess the effectiveness of the GPRI in improving patient empowerment (primary outcome), while also assessing implementation from the perspective of clinicians and the healthcare service. Patients referred for a cancer risk assessment to the conjoint clinical genetics service of two metropolitan hospitals in Victoria, Australia, who meet the eligibility criteria and consent to POETIC will be randomised to the usual care or intervention group. Those in the intervention group will complete the GPRI prior to their appointment with the screening results available for the clinicians’ use during the appointment. Appointment audio recordings, clinician reported information about the appointment, patient-reported outcome measures, and clinical data will be used to examine the effectiveness of using the GPRI. Appointment audio recordings, health economic information, and structured interviews will be used to examine the implementation of the GPRI.
Discussion: The POETIC trial takes a pragmatic approach by deploying the GPRI as an intervention in the routine clinical practice of a cancer-specific clinical genetics service that is staffed by a multidisciplinary team of genetics and oncology clinicians. Therefore, the effectiveness and implementation evidence generated from this real-world health service setting aims to optimise the relevance of the outcomes of this trial to the practice of genetic counselling while enhancing the operationalisation of the screening tool in routine practice.
Trial registration: Australian New Zealand Clinical Trials Registry registration number 12621001582842p. Date of registration: 19th November 2021.