bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Minimally Invasive Fixation Method for Flail Chest Management in a Canine Model: An Animal Research
Authors: Li Shujun , Li Zhe , Zhu Weiwei , Zhang Bing , Zhang Yaxiao , Li Huixian , Lv Baolei , Zhen Qiang , Liu Lin , Liu Lijun , Wu Yanxin 
Published: January 2023

Abstract
Background: Multiple rib fractures can lead to flail chest with up to 35% mortality rate due to severe pulmonary complications. Current treatments of flail chest remain controversial. Studies have shown that surgical treatments can improve outcomes and reduce mortality, comparing to non-operative treatments. Current surgical fixation methods focus on stabilization of ribs on the outward facing side, and they require division of intercostal muscles. Damages to surrounding nerves and vessels may lead to chronic pain. This study tests a novel interior fixation method that minimizes neurovascular injuries.
Methods: Twelve healthy canines were divided in two surgical operation groups for exterior and interior fixation using titanium metal plates. Osteotomy with oblique fractures was prepared under general anesthesia. Exterior fixation was performed in open surgery. Interior fixation was minimally invasive using custom made tools including a flexible shaft extension screwdriver, solid plate stand, guiding wire loop and metal plates with threaded holes.
Results: Respiratory and cardiovascular functions (RR, PO2, PCO2, SpO2, and HR) together with body temperature were measured before anesthesia and within 48 hours after surgery. The difference in measurements was not statistically significant between the two groups before surgery with P values greater than 0.05. However, the interior group canines had better RR and PO2 values starting from the 24th hour, and better PCO2, SpO2, and HR values starting from the 48th hour. It took longer operation time to complete the minimally invasive interior fixation surgery (P value less than 0.001), but the total blood loss was less than the exterior fixation group (P value less than 0.001). Results also showed that interior group canines suffered less pain, and they had quicker recovery in gastrointestinal and physical mobility.
Conclusions: The investigative interior fixation method was safe and effective in rib stabilization on a canine rib fracture model, comparing to the exterior fixation method. The interior fixation was minimally invasive, with less damages to tissues and nerves surrounding the ribs, leading to better postoperative outcomes.
MAOA suppresses the growth of gastric cancer by interacting with NDRG1 and regulating the Warburg effect through the PI3K/AKT/mTOR pathway
Authors: Wang Yang-Yang , Zhou Yao-Qi , Xie Jia-Xuan , Wang Shu-Chang , Li Qing , Hu Li-Peng , Jiang Shu-Heng , Yi Shuang-Qin , Xu Jia , Cao Hui , Zhao Hao , Li Jun 
Published: January 2023

Abstract
Objective
Previous studies have indicated that neurotransmitters play important roles in the occurrence and development of gastric cancer. MAOA is an important catecholamine neurotransmitter-degrading enzyme involvedin the degradation of norepinephrine, epinephrine and serotonin. To find a potential therapeutic target for the treatment of gastric cancer, the biological functions of MAOA and the underlying mechanism in gastric cancer need to be explored.
Methods
The Cancer Genome Atlas(TCGA), Gene Expression Omnibus (GEO) datasets, Kaplan‒Meier (KM) plotter and Oncomine databases were used to identify the differentially expressed genes, which mainly involved the degradation and synthesis enzymes of neurotransmitters in gastric cancer. We also investigated the expression pattern of MAOA in human and mouse tissues and cell lines by immunohistochemistry and Western blottinganalysis. Western blotting, quantitative real-time PCR, enzyme-linked immunosorbent assay (ELISA) and a Seahorse experiment were used to identify the molecular mechanism ofcancer cell glycolysis. MAOA expression and patientsurvival were analysed in the Ren Ji cohort, and univariate and multivariate analyses were performed based on the clinicopathological characteristics of the above samples.
Results
MAOA expression was significantly downregulatedin gastric cancer tissue and associated with poor patient prognosis. Moreover, the expression level of MAOA in gastric cancer tissue had a close negative correlation with theSUXmax valueof PET-CT in patients. MAOA suppressed tumour growth and glycolysis and promoted cancer cell apoptosis. We also show that MAOA can interact with NDRG1 and regulate glycolysis through suppression of the PI3K/Akt/mTOR pathway. MAOA expression may serve as an independent prognostic factor in gastric cancer patients.
Conclusions
MAOA attenuated glycolysis and inhibitedthe progression of gastric cancer through the PI3K/Akt/mTOR pathway. Loss of function or downregulation of MAOA can facilitate gastric cancer progression. Overexpression of MAOA and inhibition of the PI3K/Akt/mTOR pathway may provide a potential method for gastric cancer treatment in clinicaltherapeutic regimens.
Effects of arsenic exposure on trace element levels in the hippocampus and cortex of rats and their gender differences
Authors: Yan Xi , Zhang Jun , Li Junyu , Zhang Xinglai , Wang Yi , Chen Xiaolu , Luo Peng , Hu Ting , Cao Xueshan , Zhuang Hongbin , Tang Xiaoxiao , Yao Fang , He Zhijun , Shen Liming 
Published: January 2023

Abstract

Arsenic (As) exposure is a major public health challenge worldwide. Much evidence suggests that As exposure can cause disturbances in trace element balance in the body. However, there are few reports on the effect of As on trace element levels in the central nervous system (CNS). We conducted an experiment to detect 19 elements in the hippocampus and cortex of arsenic-exposed rats by inductively coupled plasma mass spectrometry. The results showed that As levels in the hippocampus and cortex of arsenic-exposed rats were significantly higher than those in the control group, and As levels in the cortex were significantly higher than that in the hippocampus. In the hippocampus of arsenic-exposed group, the levels of Cd, Ho and Rb increased, while the levels of Au, Ba, Ce, Cs, Pd, Se, Sr and Tl decreased; In cortex, the levels of Cd and Rb increased, and the levels of Se and Au decreased. There were significant sex differences in the effects of As on Cd, Ba, Rb and Sr in the hippocampus and on Cd and Mo in the cortex. It is suggested that elemental imbalance may be a risk factor for the development of As toxicity and is closely associated with arsenic-induced CNS damage.
Development of a gas chromatography-mass spectrometry method for determination of β-propiolactone (BPL) residues in inactivated covid-19 vaccines
Authors: Mirjalili Seyedeh Zohreh , Tamiji Zahra , Shirangi Mehrnoosh , Amini Mohsen 
Published: January 2023

Abstract
β-propiolactone (BPL) is a common inactivator agent used in vaccines. Due to BPL carcinogenicity, complete hydrolysis of it is necessary to prevent cytotoxicity in mammalian cells. As a result, more attention should be paid to it at the clinic and it is important to measure its trace amounts. BPL analysis is challenging due to its instability. A simple and fast gas chromatography-mass spectrometry (GC-MS) method was developed for quantitation of residual BPL in inactivated covid-19 vaccines. Caprolactone was used as internal standard in samples solutions, the analysis was performed after extraction of analyte from vaccine media by ethyl acetate. The validity of the method was studied with a linearity of r2 > 0.99 over the concentration range of 0.2–20 µg/mL with the limit of detection and the limit of quantification of 0.68 and 2.06 µg/mL, respectively. The target analyte BPL was not detected in the samples, demonstrating the test samples were qualified. The established method can be used for quality control of inactivated covid-19 vaccines.
Composition of mucus- and digesta-associated bacteria in growing pigs with and without diarrhea differed according to the presence of colonic inflammation
Authors: Panah Farhad M. , Lauridsen Charlotte , Højberg Ole , Elvang Henrik , Nielsen Tina Skau 
Published: January 2023

Abstract
Background
In the pig production, diarrhea can occur during different growth stages including the period 4–16 weeks post weaning, during which diarrhea a diarrheal outbreak also termed as colitis-complex diarrhea (CCD) can occur and it is distinct from post-weaning diarrhea (1–2 weeks post weaning). We hypothesized that CCD in growing pigs is associated with changes in colonic microbiota composition and fermentation patterns, and the aim of the present observational study was to identify changes in digesta-associated bacteria (DAB) and mucus-associated bacteria (MAB) in the colon of growing pigs with and without diarrhea. A total of 30 pigs (8–12 weeks of age) were selected; 20 showed clinical signs of diarrhea and 10 appeared healthy. Based on histological examination of colonic tissue, 21 pigs were selected for further studies and classified as follows: without diarrhea, no colon inflammation (NoDiar; n = 5), with diarrhea, without inflammation (DiarNoInfl; n = 4), and with diarrhea, with inflammation (DiarInfl; n = 12). Composition (16S rRNA gene amplicon sequencing) and fermentation pattern (SCFA profile) of the DAB and MAB communities were characterized.
Results
The DAB showed higher alpha diversity compared to MAB in all pigs, and both DAB and MAB showed lowest alpha diversity in the DiarNoInfl group. Beta diversity was significantly different between DAB and MAB as well as between diarrheal groups in both DAB and MAB. Compared to NoDiar, DiarInfl showed increased abundance of various taxa, incl. certain pathogens, in both digesta and mucus, as well as decreased digesta butyrate concentration. However, DiarNoInfl showed reduced number of genera compared to NoDiar, but still lower butyrate concentration.
Conclusion
Diversity and composition of MAB and DAB changed in diarrheal groups dpending on presence/absence of colonic inflammation. We also suggestion that DiarNoInfl group was at the earlier stage of diarrhea compared with DiarInfl and may as well highlight the overall importance of butyrate in relation to gut health. In conclusion, we confirmed that changes in DAB and MAB were associated with CCD and reduced butyrate concentration in digesta. Moreover, DAB might suffice for future community-based studies of CCD.
Promising conilon coffee trees clones for agroforestry and intercropping systems
Authors: Senra João Felipe de Brites , Silva Josimar Aleixo da , Esposti Marlon Dutra Degli , Dan Maurício Lima , Zacarias Alex Justino , Conceição Amanda Oliveira , Milheiros Idalina Sturião , Silva Uliana Ribeiro 
Published: January 2023

Abstract

This research aimed to select conilon coffee tree clones (Coffea canephora Pierre ex A.Froehner) for agroforestry and/or intercropping systems. The experiment was carried out at Bananal do Norte Experimental Farm (20º45' S and 41º17' W), Espírito Santo, Brazil. The clones were evaluated in an agroforestry system with “urograndis” hybrid eucalyptus trees (Eucalyptus grandis W.Hill × E. urophylla S.T.Blake) plus prata-type banana herbs (Musa spp.) and an intercropping with ‘Bahia’ sweet orange trees (Citrus sinensis (L.) Osbeck). An augmented block experiment design, with two plants per plot, four controls, in six blocks in the agroforestry system and four in the intercropping, was adopted. Thirteen morphophysiological characteristics were evaluated. Data analysis was performed using the restricted maximum likelihood method and best linear unbiased prediction and the significance of genetic effects by the likelihood ratio test. To select superior clones the Mulamba-Rank index was performed. Twenty clones were selected for the agroforestry and 20 for the intercropped system. The clones 16, 17, 35, 43, 48, 61, 64, 68 and T3 were the most promising for both systems. There is genetic variability to be explored among the clones, being possible the development of varieties, in the future, for agroforestry and intercropping systems.
Thoracoscopic Treatment of Mediastinal Ectopic Parathyroid Adenomas: a Case Series and Literature Review
Authors: Chavez Karla Veronica , Chavez-Tostado Mariana , Bolaños-Morales Francina Valezka , Lopez-Alamillo Susana 
Published: January 2023

Abstract
Purpose: hyperparathyroidism (HPT) is a disease caused by hypersecretion of one or more parathyroid glands, it can be associated with ectopic mediastinal parathyroid glands (MEPA) in 2% of cases. The use of video-assisted thoracoscopic surgery (VATS) for the surgical resection of these glands is a safe, cost-effective, and low morbidity option for patients with MEPA. We report a case series of patients with this disease managed with VATS.
Methods: From 2008 to 2022, a retrospective study involving patients with MEPA and treated by VATS approach was performed in a tertiary hospital in Mexico city. Relevant biochemical and clinical variables such as imaging studies, pre and postoperative lab results, surgical strategy and outcomes and pathological analysis were analyzed.
Results: Four cases of mediastinal parathyroid adenomas causing HPT were included. All patients were female with a median age of 52.5 years-old (range 46-59 years), half of the patients had primary HPT and the others tertiary HPT after kidney transplant. 75% of cases had a MEPA in the medium mediastinum, and all had a preoperative positive SPECT-CT 99mTc Sestamibi scan. Mean preoperative PTH was 621.3pg/mL (182-1382pg/mL). All patients successfully underwent parathyroidectomy with a VATS approach, no deaths were reported.
Conclusions: VATS is a minimally invasive surgery that provides adequate access to mediastinal located glands, optimal visualization of mediastinal structures and has a high resection success rate with less complications and morbidity than open approaches.
Amine-decorated zirconium-based metal-organic framework for ultrafast detection of 2, 4, 6-trinitrophenol in aqueous medium
Authors: Verma Rajpal , Dhingra Gaurav , Kaur Manpreet , Garg Deepika , Mohiuddin Irshad , Malik Ashok 
Published: January 2023

Abstract
An amine-decorated zirconium-based metal–organic framework (MOF, coded as UiO-66-NH2) with rod shaped morphology has been solvothermally synthesized using low-cost and easily available 2-aminoterephthalic acid as an organic linker. The crystallinity of the synthesized MOF material has been confirmed by sharp PXRD diffraction pattern. Photoluminescent studies showed that the UiO-66-NH2 features a strong fluorescent behaviour when dispersed in water, which could be used as a highly selective and sensitive sensor for detection of trace amount of 2,4,6-trinitrophenol (TNP), even in coexistence with other potentially competitive nitroaromatic analytes. High value of Stern-Volmer quenching constant Ksv (1.106 x 105 M-1), photoluminescent quenching efficiency (97.8%) and lower detection limit (0.95 μM/217ng mL-1) ascertained extraordinary sensitivity of developed UiO-66-NH2 for TNP. Density functional theory (DFT) calculations and electrostatic interactions (i.e. ionic interaction, H-bonding and π-π interaction) indicated that electron and energy transfer processes play a key role in the turn-off quenching response of UiO-66-NH2 sensor towards TNP.
What is expected for China’s SARS-CoV-2 epidemic?
Authors: HERNANDEZ-SUAREZ CARLOS , Murillo-Zamora Efrén 
Published: January 2023

Abstract
Recently, China announced that its “zero-covid’ policy would end, which will bring serious challenges to the country’s health system. In here we provide simple calculations that allows us to provide an estimate of what is expected as an outcome in terms of fatalities, using the fact that it is a highly contagious disease that will expose most of a highly vaccinated population to the virus. We use recent findings regarding the amount of reduction in the risk of severe outcome achieved by vaccination and arrive to an estimate of 1.1 m deaths, 60% of these are males. In our model, 84% percent of deaths occur in individuals with age 55 years or older. In a scenario in which this protection is completely lost due to waning and the infection fatality rate of the prevalent strain reaches similar levels to the observed in the beginning of the epidemic, the death toll could reach 2.4 m, 93% in 55 years or older.
Effect of Plasma Liquid on Colloidal (CoNPs) and (PtNPs) Nanostructures Produced by Laser Ablation Technique for Antibacterial Applications
Authors: Khamees Entidhar Jasim , Azeez Halah Mohammed , Hussein Ahmed Shaker , Ahmed Rafea T. , Gençyılmaz Olcay 
Published: January 2023

Abstract
In this work, the biological and physical properties of colloidal platinum (PtNPs) and cobalt (CoNPs) nanoparticles were examined for antibacterial applications. The colladial PtNPs and CoNPs nanoparticles were produced using two different liquids (double-distilled water (DDDW) and Dulbecco’s Modified Eagle’s Medium (DMEM)) by laser ablation technique (PLAL). Characterization techniques such as transmission electron microscopy (TEM), UV-vis spectroscopy and Fourier transform infrared (FTIR) were used to determine some physical properties of PtNPs and CoNPs. The antibacterial effects ofcolladial PtNPs and CoNPs at different doses on various bacteria were investigated and their antibacterial activity resistances were calculated by measuring the inhibition zone widths. The effect of the liquid used in the production, the type of bacteria and dose amounts on the antibacterial resistance of these nanoparticles were determined. In this study, it has been shown that the liquid used in the production of PtNPs and CoNPs can create significant changes in the physical and antibacterial properties of nanoparticles, especially by changing the size and concentration of collodial PtNPs.
Gattung Alchemilla im Französischen und Schweizer Jura
Authors: Hügin Gerold , Fröhner Sigurd Erich , Purschke Christoph 
Published: January 2023

In Karten wird die Verbreitung von 49 Alchemilla-Arten im Französischen und Schweizer Jura dargestellt. A. psilopodia wird als Art neu beschrieben. Mehrere Erstnachweise werden erbracht: Neu sind A. oscensis für Mitteleuropa, A. hybrida für die Schweiz, A. jaquetiana für Frankreich, A. firma, A. propinqua, A. speciosa, A. squarrosula für den Jura. Die aus Südwesteuropa einstrahlenden Arten (A. hybrida, A. oscensis, A. vetteri) werden ausführlich beschrieben und in Abbildungen, Bestimmungs- und Merkmalstabellen mit ähnlichen Arten verglichen (A. colorata, A. exigua, A. filicaulis, A. glaucescens, A. monticola, A. plicata, A. propinqua, A. psilopodia, A. strigosula, A. subglobosa).
Ranunculus chrysoleptos Brodtb. & Dunkel (Ranunculaceae) – eine neue Art aus dem Ranunculus-auricomus-Komplex
Authors: Dunkel Franz Georg 
Published: January 2023

Ranunculus chrysoleptos BRODTB. & DUNKEL wird beschrieben und abgebildet. Die Hauptverbreitung der Art liegt in der Oberrheinischen Tiefebene um Karlsruhe.
Arten aus dem Ranunculus-auricomus-Komplex (Ranunculaceae) in Deutschland
Authors: Dunkel Franz Georg 
Published: January 2023

Fünf neue Arten aus dem Ranunculus-auricomus-Komplex in Deutschland werden beschrieben und abgebildet. R. pleiophyllus ist weiter verbreitet und bislang aus Rheinland-Pfalz, Baden-Württemberg, Bayern, Saarland und Mecklenburg-Vorpommern nachgewiesen. Die übrigen neuen Arten besitzen – soweit bis jetzt bekannt – kleine Areale: R. excisus in Rheinland-Pfalz, R. vertumnaliformis in Mecklenburg-Vorpommern, R. biclaterae in Nordbayern und R. ferocior in Mecklenburg-Vorpommern und Nordbayern.
Applicability of the HBV model to a human-influenced catchment in northern China
Authors: Wang Yueyang , Wang Yanjun , Wang Yan , Li Chenxi , Ju Qin , Jin Junliang , Deng Xiyuan , Sun Gaoxia , Bao Zhenxin 
Published: January 2023

Abstract
The Hydrologiska Byrans VattenbalansavdeIning (HBV) model is a catchment hydrological model that has been widely applied to hundreds of catchments worldwide. Taking the Nanjuma River Basin which is located in a semi-arid climate zone and has been highly regulated by human activities, as a case, the applicability of the HBV model to the basin was investigated. Results show that (1) due to environmental change, recorded stream flow of the Nanjuma River presented a significant decreasing trend, and the relationship between runoff and precipitation was changed as well, with the correlation coefficient decreasing from 0.58 in the natural period of 1961–1979 to 0.01 in a highly regulated period of 2000–2018. (2) The HBV model performs well on daily and monthly discharge simulation for the natural period with the Nash–Sutcliffe efficiency (NSE) coefficients in calibration and validation periods of 0.63 and 0.81 for daily discharge simulation. (3) The HBV model's applicability would like to decrease when the Nanjuma River Basin was moderately and intensively regulated by human activities. The daily-scale NSEs in moderate-disturbance and intensive-disturbance phases are 0.42 and −0.3, which means the HBV model almost lost its capacity in capturing hydrological features for a highly regulated catchment.
Rise of Bacterial Small Proteins and Peptides in Therapeutic Applications
Authors: Meenakshi Shanmugaraja , Shelin Ruby 
Published: January 2023

Background:
Polypeptides that comprise less than 100 amino acids (50 amino acids in some
cases) are referred to as small proteins (SPs), however, as of date, there is no strict definition. In
contrast to the small polypeptides that arise due to proteolytic activity or abrupt protein synthesis, SPs
are coded by small open reading frames (sORFs) and are conventionally synthesized by ribosomes.

Purpose of the Review:
Although proteins that contain more than 100 amino acids have been studied
exquisitely, studies on small proteins have been largely ignored, basically due to the unsuccessful
detection of these SPs by traditional methodologies/techniques. Serendipitous observation of several
small proteins and elucidation of their vital functions in cellular processes opened the floodgate of a
new area of research on the new family of proteins called "Small proteins". Having known the
significance of such SPs, several advanced techniques are being developed to precisely identify and
characterize them.

Conclusion:
Bacterial small proteins (BSPs) are being intensely investigated in recent days and that
has brought the versatile role of BSPs into the limelight. In particular, identification of the fact that
BSPs exhibit antimicrobial activity has further expanded its scope in the area of therapeutics. Since the
microbiome plays an inevitable role in determining the outcome of personalized medicine, studies on
the secretory small proteins of the microbiome are gaining momentum. This review discusses the
importance of bacterial small proteins and peptides in terms of their therapeutic applications.
Remodeling of the Gut Microbiota in Colorectal Cancer and its Association
with Obesity
Authors: Damavandi Sedigheh , Avan Amir , Zafari Nima , Velayati Mahla , Mehrabadi Shima , Khazaei Majid , Hassanian Seyed Mahdi , Ferns Gordon A. 
Published: January 2023

Abstract:
The considerable burden of colorectal cancer and the increasing prevalence in young adults emphasizes
the necessity of understanding its underlying mechanisms and risk factors as well as providing more effective
treatments. There is growing evidence of a positive relationship between obesity and colorectal cancer. Furthermore,
the prominent role of gut microbiota dysbiosis in colorectal carcinogenesis is becoming more evident.
Sequencing studies demonstrate an altered composition and ecology of intestinal microorganisms in both
colorectal cancer and obese patients and have pinpointed some specific bacteria as the key role players. The purpose
of this review is to provide a general outlook of how gut microbiota may impact the initiation and promotion
of colorectal cancer and describes probable links between gut microbiota and obesity. We also provide evidence
about targeting the microbiota as an intervention strategy for both ameliorating the risk of cancer and augmenting
the therapy efficacy.