bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intramyocardial cell-based therapy with Lomecel-B during bidirectional cavopulmonary anastomosis for hypoplastic left heart syndrome: the ELPIS phase I trial
Authors: Kaushal Sunjay , Hare Joshua M , Hoffman Jessica R , Boyd Riley M , Ramdas Kevin N , Pietris Nicholas , Kutty Shelby , Tweddell James S , Husain S Adil , Menon Shaji C , Lambert Linda M , Danford David A , Kligerman Seth J , Hibino Narutoshi , Korutla Laxminarayana , Vallabhajosyula Prashanth , Campbell Michael J , Khan Aisha , Naioti Eric , Yousefi Keyvan , Mehranfard Danial , McClain-Moss Lisa , Oliva Anthony A , Davis Michael E 
Published: January 2023

AbstractAimsHypoplastic left heart syndrome (HLHS) survival relies on surgical reconstruction of the right ventricle (RV) to provide systemic circulation. This substantially increases the RV load, wall stress, maladaptive remodelling, and dysfunction, which in turn increases the risk of death or transplantation.Methods and resultsWe conducted a phase 1 open-label multicentre trial to assess the safety and feasibility of Lomecel-B as an adjunct to second-stage HLHS surgical palliation. Lomecel-B, an investigational cell therapy consisting of allogeneic medicinal signalling cells (MSCs), was delivered via intramyocardial injections. The primary endpoint was safety, and measures of RV function for potential efficacy were obtained. Ten patients were treated. None experienced major adverse cardiac events. All were alive and transplant-free at 1-year post-treatment, and experienced growth comparable to healthy historical data. Cardiac magnetic resonance imaging (CMR) suggested improved tricuspid regurgitant fraction (TR RF) via qualitative rater assessment, and via significant quantitative improvements from baseline at 6 and 12 months post-treatment (P &lt; 0.05). Global longitudinal strain (GLS) and RV ejection fraction (EF) showed no declines. To understand potential mechanisms of action, circulating exosomes from intramyocardially transplanted MSCs were examined. Computational modelling identified 54 MSC-specific exosome ribonucleic acids (RNAs) corresponding to changes in TR RF, including miR-215-3p, miR-374b-3p, and RNAs related to cell metabolism and MAPK signalling.ConclusionIntramyocardially delivered Lomecel-B appears safe in HLHS patients and may favourably affect RV performance. Circulating exosomes of transplanted MSC-specific provide novel insight into bioactivity. Conduct of a controlled phase trial is warranted and is underway.Trial registration number NCT03525418.
Comparison of meta-analytical estimates of outcomes after Alfieri or neochordal repair in isolated anterior mitral prolapse
Authors: Khairallah Sherif , Rahouma Mohamed , Dabsha Anas , Demetres Michelle , Gaudino Mario Fl , Mick Stephanie L 
Published: January 2023

Abstract

OBJECTIVES
Repair of the isolated degenerative anterior mitral leaflet has been considered more challenging and associated with compromised durability compared with isolated posterior leaflet in major series. Implantation of neochordae or Alfieri edge-to-edge is the most employed repair technique for isolated anterior repair currently, but little data exist comparing their relative durability. We sought to investigate this issue with this meta-analysis.

METHODS
A literature search was performed (Ovid MEDLINE, Ovid Embase and The Cochrane Library). The primary outcome was the incidence rate (IR) of reoperation, the secondary outcomes were recurrent moderately severe/severe mitral regurgitation (MR), in-hospital/30-day reoperation and mortality and follow-up mortality. A random-effect model was used. Leave-one-out, subgroup analysis (Alfieri versus neochordae) and meta-regression were done.

RESULTS
Seventeen studies (including 1358 patients) were included. At a weighted mean follow-up of 5.56 ± 3.31 years, the IR for reoperation was 14.45 event per 1000 person-year and significantly lower in Alfieri than neochordae repair (9.40 vs 18.61, P = 0.04) on subgroup analysis. The IR of follow-up moderately severe/severe MR was 19.89 event per 1000 person-year and significantly lower in Alfieri than neochordae repair (10.68 and 28.63, P = 0.01). In a sensitivity analysis comparing homogenous studies, a significant difference in the recurrence of regurgitation in favour of the Alfieri approach remained. There were no differences in operative outcomes or survival. There were significant associations between increased incidence of late reoperation and New York Heart Association class III/IV and associated coronary artery bypass graft procedure for whole cohort.

CONCLUSIONS
Alfieri repair may be associated with a lower incidence of recurrent MR compared with neochordae-based repair in the setting of isolated degenerative anterior mitral pathology. This is the first such meta-analysis and further inquiry into this area is needed.
Elevated sphingosine-1-phosphate lyase leads to increased metabolism and reduced survival in adrenocortical carcinoma
Authors: Williams Jack L , Smith Chris , Hall Charlotte , Khaled Zakaa , Maharaj Avinaash , Kwong Ruth , Pittaway James , Casas Josefina , Parvanta Laila , Abdel-Aziz Tarek Ezzat , Palazzo Fausto , Chung Teng-Teng , Guasti Leonardo , Metherell Lou , Prasad Rathi 
Published: January 2023

Abstract

Objective
Adrenocortical carcinomas (ACCs) are invasive tumours arising in the adrenal cortex, and steroidogenic tumours are associated with worse prognostic outcomes. Loss-of-function mutations in sphingosine-1-phosphate lyase (SGPL1) cause primary adrenal insufficiency and as a key degradative enzyme in the sphingolipid pathway, SGPL1 also influences the balance of pro-proliferative and pro-apoptotic sphingolipids. We, therefore, hypothesized increased SGPL1 may be linked to increased disease severity in ACC.

Design
Analyse SGPL1 expression impact on patient survival and adrenal cancer cell phenotype. We analysed two ACC cohorts with survival and corresponding transcriptomic data, focusing on SGPL1 and sphingolipid pathway genes. In vitro, we generated SGPL1-knockout and overexpressing H295R adrenocortical cells to investigate the role of SGPL1 in cell signalling in ACCs.

Results
We found increased expression of several sphingolipid pathway receptors and enzymes, most notably SGPL1 correlated with reduced patient survival in both cohorts. Overexpression of SGPL1 in the H295R cell line increased proliferation and migration while reducing apoptosis, while SGPL1 knockout had the opposite effect. RNA-seq revealed a global increase in the expression of genes in the electron transport chain in overexpressing cells, correlating with increased aerobic respiration and glycolysis. Furthermore, the opposite phenotype was seen in cells lacking SGPL1. We subsequently found the increased proliferation is linked to metabolic substrate availability and increased capacity to use different fuel sources, but particularly glucose, in overexpressing cells.

Conclusions
We, therefore, propose that SGPL1-overexpressing ACC tumours reduce patient survival by increasing fuel usage for anabolism and energy production to facilitate growth and invasion.
What is the impact of forced displacement on health? A scoping review
Authors: Cuadrado Cristóbal , Libuy Matías , Moreno-Serra Rodrigo 
Published: January 2023

Abstract
While there is a broad literature analysing the effects of migration on health, important knowledge gaps persist particularly on the causal effects of forced displacement on health outcomes. We undertake a scoping review of applied epidemiological, statistical and econometric studies examining causal health impacts of forced displacement, which initially identified 1454 studies from the health and social sciences disciplines published up to May 2021. Our study makes two key contributions. First, we offer a comprehensive overview of the evidence generated, methodologies adopted and analytical challenges faced by current research examining the causal relationship between forced displacement and health. Second, we present concrete examples of how key challenges around study design and estimation approaches influence the strength of the evidence-base on the topic, using as a case study the broad domain of reproductive health. We find that, beyond the increased mortality risk that can be attributed to forced displacement, most of the available empirical evidence for a wide range of health outcomes is prone to substantial bias, making it difficult to draw firm conclusions. Our synthesis of credible studies conducted in different settings indicates that current research practice in the field could be strengthened through selection of valid control groups and application of more appropriate causal inference methods. Our findings are useful to promote the generation of further evidence on the topic that can reliably inform the design of policies to protect the health of displaced populations.
Effect of the biosynthesis of the volatile compound phenylacetaldehyde on chloroplast modifications in tea (Camellia sinensis) plants
Authors: Zeng Lanting , Zhou Xiaochen , Fu Xiumin , Hu Yilong , Gu Dachuan , Hou Xingliang , Dong Fang , Yang Ziyin 
Published: January 2023

Abstract
Plant volatile compounds have important physiological and ecological functions. Phenylacetaldehyde (PAld), a volatile phenylpropanoid/benzenoid, accumulates in the leaves of tea (Camellia sinensis) plants grown under continuous shading. This study was conducted to determine whether PAld production is correlated with light and to elucidate the physiological functions of PAld in tea plants. Specifically, the upstream mechanism modulating PAld biosynthesis in tea plants under different light conditions as well as the effects of PAld on chloroplast/chlorophyll were investigated. The biosynthesis of PAld was inhibited under light, whereas it was induced in darkness. The structural gene encoding aromatic amino acid aminotransferase 1 (CsAAAT1) was expressed at a high level in darkness, consistent with its importance for PAld accumulation. Additionally, the results of a transcriptional activation assay and an electrophoretic mobility shift assay indicated CsAAAT1 expression was slightly activated by phytochrome-interacting factor 3-2 (CsPIF3-2), which is a light-responsive transcription factor. Furthermore, PAld might promote the excitation of chlorophyll in dark-treated chloroplasts and mediate electron energy transfer in cells. However, the accumulated PAld can degrade chloroplasts and chlorophyll, with potentially detrimental effects on photosynthesis. Moreover, PAld biosynthesis is inhibited in tea leaves by red and blue light, thereby decreasing the adverse effects of PAld on chloroplasts during daytime. In conclusion, the regulated biosynthesis of PAld in tea plants under light and in darkness leads to chloroplast modifications. The results of this study have expanded our understanding of the biosynthesis and functions of volatile phenylpropanoids/benzenoids in tea leaves.
A Critical Study on the Potential of Solid-Phase Fluorescence Spectroscopy (SPFS) in Quantitative Analysis: Effect of Solid Diluent and Sampling Method on Signal Quality
Authors: Öztürk Merve , Gezici Orhan 
Published: January 2023

Abstract

Background
Solid-phase fluorescence spectroscopy (SPFS) is a very useful non-destructive technique for directly analyzing samples in solid form without the use of solvents. However, due to the so-called inner-filter effect, it is sometimes necessary to dilute solid samples using non-fluorescent solids as diluents.

Objective
This study aimed to explore the potential of SPFS in the quantitative analysis of fluorescent species based on: (1) the type of solid diluent; and (2) the sampling method used in the SPFS analysis.

Methods
Four different solids were used as solid diluents in the preparation of standard mixtures having different concentrations of rhodamine b and fluorescein as model compounds. Standard mixtures of model compounds were sampled by two different methods called: (1) the powder-cell method; and (2) the adhesive tape method. LOQ and calibration sensitivity calculated from the calibration graphs were used to assess the measurement performance. The usability of SPFS in real-sample analyses was also evaluated in detail.

Results
Among the solid diluents studied, the best results were obtained with sodium carbonate. The powder-cell method yielded a significant advantage over the adhesive tape method. The lowest LOQs for rhodamine b and fluorescein were obtained by sodium carbonate and the powder-cell method as 0.06 and 0.11 mg/kg, respectively. The results of real-sample analyses were verified using conventional liquid-phase fluorescence spectroscopy (LPFS).

Conclusion
Solid-diluent type and sampling method were found to affect the performance of the SPFS technique. A combination of sodium carbonate and the powder-cell method gave the best results. According to the t-test, no difference was observed between the means obtained by SPFS and LPFS techniques in real-sample analyses.

Highlights
In SPFS, toxic organic solvents and difficult sample preparation steps are not required. This makes the method advantageous over conventional fluorescence analyses performed in the liquid phase.
Financial centre primacy around the world: international analysis based on mergers and acquisitions data
Authors: Wójcik Dariusz , Keenan Liam , Pažitka Vladimír , Urban Michael , Wu Wei 
Published: January 2023

AbstractWe analyse mergers and acquisitions in the financial sector between 2000 and 2017 to explore the domestic hierarchies of financial centres. Across a sample of 16 countries, we reveal different levels of financial centre primacy and explain how these levels change over time. These findings are analysed through a theoretical framework which integrates the literatures on urban primacy, global and world cities and comparative political economy. Situating our findings at the intersection of these literatures allows us to remain sensitive to geography, history and institutions in our analysis of financial centre primacy. Overall, our results show uneven levels of financial centre primacy around the world and that primate financial centres do not universally increase their dominance over time.
Cultures as networks of cultural traits: a unifying framework for measuring culture and cultural distances
Authors: De Benedictis Luca , Rondinelli Roberto , Vinciotti Veronica 
Published: January 2023

AbstractUsing data from the sixth wave of the World Value Survey and operationalising a definition of national culture that emphasises both specific cultural traits and the inter-dependence among them, this paper proposes a methodology to reveal the latent network structure of every national culture and to measure the cultural distance associated with every pair of countries as a Jeffreys’ divergence between copula graphical models. The two components of this new measure of cultural distance show different correlations with measures of geographical, historical, economic, and political distance among countries and with the similarity in the topologies of the countries cultural networks.
Responses of key root traits in the genusOryzato soil flooding mimicked by stagnant, deoxygenated nutrient solution
Authors: Tong Shuai , Kjær Johan Emil , Peralta Ogorek Lucas León , Pellegrini Elisa , Song Zhiwei , Pedersen Ole , Herzog Max 
Published: January 2023

AbstractExcess water can induce flooding stress resulting in yield loss, even in wetland crops such as rice (Oryza). However, traits from species of wild Oryza have already been used to improve tolerance to abiotic stress in cultivated rice. This study aimed to establish root responses to sudden soil flooding among eight wild relatives of rice with different habitat preferences benchmarked against three genotypes of O. sativa. Plants were raised hydroponically, mimicking drained or flooded soils, to assess the plasticity of adventitious roots. Traits included were apparent permeance (PA) to O2 of the outer part of the roots, radial water loss, tissue porosity, apoplastic barriers in the exodermis, and root anatomical traits. These were analysed using a plasticity index and hierarchical clustering based on principal component analysis. For example, O. brachyantha, a wetland species, possessed very low tissue porosity compared with other wetland species, whereas dryland species O. latifolia and O. granulata exhibited significantly lower plasticity compared with wetland species and clustered in their own group. Most species clustered according to growing conditions based on PA, radial water loss, root porosity, and key anatomical traits, indicating strong anatomical and physiological responses to sudden soil flooding.
Slow mode oscillations in curved arcade loops
Authors: Lopin I , Nagorny I 
Published: January 2023

ABSTRACT
In this work we theoretically investigate the effect of curvature on the slow-mode oscillations in coronal loop arcade. A simple model of an arc circle magnetic slab is used to simulate curved coronal magnetic structures. Solving the set of magnetohydrodynamic (MHD) equations for a compressible plasma, we obtain the dispersion relation that governs the compressible MHD modes in the model. A band of slow body modes with phase speeds close to the internal tube speed and a single hybrid slow mode with phase speed close to the external tube speed are found to exist under typical coronal circumstances. The principal slow body and hybrid modes both produce radial kink-like displacements of the slab axis and distort its cross-section. These motions are accompanied with the dominating longitudinal oscillations. Such mode properties may result in Doppler shift and intensity oscillations as well as oscillating spatial displacements, observed in coronal loops. A number of observations of long-period oscillations in arcade loops are interpreted on the basis of the developed theory.
Clinical and Public Health Considerations for HPV Vaccination in Midadulthood: A Narrative Review
Authors: King Laura M , Lewnard Joseph A , Niccolai Linda M 
Published: January 2023

Abstract
Human papillomavirus (HPV) is an important cause of anogenital and oropharyngeal cancers, anogenital warts, and recurrent respiratory papillomatosis. Beginning in 2019, US guidelines recommended shared clinical decision-making (SCDM) for HPV vaccination among midadults (27–45 years). We conducted a narrative review of existing literature on HPV vaccination in midadults. The available evidence demonstrates that HPV vaccination in midadults is safe, efficacious, and likely to benefit both HPV-naïve midadults and those with previous infections. However, gaps in knowledge related to HPV vaccination have been identified among clinicians and midadult patients. Universal midadult HPV vaccination in the United States could avert 20 934–37 856 cancer cases over 100 years, costing $141 000–$1 471 000 per quality-adjusted life-year gained. Wide variation in these estimates reflects uncertainties in sexual behavior, HPV natural history, and naturally acquired immunity. Greater awareness among clinicians and midadult patients and broad implementation of SCDM may accelerate progress toward eliminating HPV-associated cancers and other diseases.
Love and Human Rights
Authors: Douglas Benedict 
Published: January 2023

Abstract
This article explains and critiques the protection of love within judgments concerning relationships under the Human Rights Act 1998. Using theory of emotion to conduct doctrinal analysis of the protection of love within international human rights laws and under the Human Rights Act 1998, it reveals a shift in the conception of love underlying the domestic judicial application of huamn rights. Whereas previously the law was underpinned by values of duty and property, judgments concerning relationships now protect the capacity of individuals to choose how to live. However, the protection of this modern conception of love is limited by judicial deference, allowing the values underpinning the historical conception of love to continue to influence the law.
Planning for the majorities: are the charging needs and preferences of electric vehicle early adopters similar to those of mainstream consumers?
Authors: Lavieri Patricia Sauri , de Oliveira Gabriel Jurado Martins 
Published: January 2023

Abstract
The mass deployment of electric vehicles (EVs) may bring significant challenges to the electricity sector. However, many of these challenges can be converted into opportunities depending on how and when consumers decide to charge their vehicles. While there are currently multiple efforts worldwide investigating EV charging behaviour, these efforts measure the behaviour of EV early adopters and may not represent the actual behaviour of the mainstream consumer. The current study uses data from a survey with near a thousand Australian consumers to shed light on the potential similarities and differences between the charging needs and preferences of EV early adopters and mainstream consumers. We find that consumer groups vary in terms of charging needs, perceived access to residential charging, and acceptance of direct charging control and management by suppliers. Our conclusions point to (i) the need for campaigns that increase the awareness and understanding of residential EV charging by mainstream consumers; (ii) the significant interest across all consumer groups in free workplace charging, which could together with residential demand management strategies leverage the use of solar energy for charging; and (iii) the need for utility plans and management strategies that enhance the mainstream consumer sense of control over charging together with their perceived monetary savings.
Exploring the perspective of patients with immune-mediated inflammatory diseases and care providers on the use of immunomodulatory drugs in infections: an interview study
Authors: Opdam Merel A A , Vriezekolk Johanna E , Broen J , den Broeder Alfons A , Verhoef Lise M 
Published: January 2023

Abstract

Objectives
Immunomodulatory agents are safe and effective as treatment for various immune-mediated inflammatory diseases (IMIDs), but are associated with a slightly increased infection risk. It is uncertain whether, in the event of an infection, continuation or temporary interruption of immunomodulatory agents leads to better outcomes. Owing to this uncertainty, it is of importance to explore the perspectives of health-care providers (HCPs) and patients on this topic. In this study, we set out to identify and provide an overview of reasons for both treatment strategies.

Methods
Semi-structured interviews were conducted with HCPs involved in the pharmacological treatment of IMIDs and with IMID patients using one or more immunomodulatory agent. Purposive sampling was used to enrich data variation. Interviews were conducted until data saturation was reached and subsequently analysed using qualitative content analysis.

Results
In total, 13 HCPs and 19 IMID patients were interviewed. A wide range of reasons for both treatment strategies were identified, categorized into 10 overarching themes, including IMID characteristics, infection characteristics and the patient–HCP relationship.

Conclusion
In this interview study, we identified various reasons for continuation or temporary interruption of immunomodulatory agents during infections for both IMID patients and HCPs. We found overlapping themes, such as IMID characteristics; however, the content and interpretation of these themes might differ between HCPs and patients. Both HCPs and patients mentioned that the decision for a treatment strategy is often about weighing benefits against risks (e.g. infection severity vs disease flare).