bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supplementary Data from <i>NAMPT</i> Is a Potent Oncogene in Colon Cancer Progression that Modulates Cancer Stem Cell Properties and Resistance to Therapy through Sirt1 and PARP
Authors: Lucena-Cacace Antonio , Otero-Albiol Daniel , Jiménez-García Manuel P. , Muñoz-Galvan Sandra , Carnero Amancio 
Published: April 2023

<p>Figure S1: Overall survival probability of the patients according to NAMPT levels segregated by colon cancer stage. Figure S2. Tumorigenic properties induces by NAMPT Figure S3. Effect of a second shRNA against NAMPT in the tumorigenic capability of tumor cells. Figure S4. CD133+ single cell derived tumorspheres of HCT116. Figure S5.Tumorigenic capabilities NAMPT-dependent in HCT 116 +/+ p53 wild type. Figure S6. (A) GSE34053 database, from human colon tumors, have transcription profiles of sorted CD133+ cells. We analyzed the correlation of CD133+ cells with NAMPT expression along with the following: HES1, OCT4 and SOX9. (B) GSE14774 database includes transcription profiles of a supplementary colon cancer cell line, HT-29, both in monolayer culture and tumorspheres. We analyzed the correlation of NAMPT on either monolayer or tumorspheres along with the following: HES1, OCT4 and SOX9. (C, left graph) Clonal heterogeneity analysis reveals that 1 mM NMN can rescue the number of holoclones up to vector levels in both cell lines. (C, right graph) Clonal heterogeneity analysis reveals that treatment with 60 ng/mL visfatin results in the same effect over holoclones. Figure S7. Effect of the addition of the NAMPT product NMN, or the extracellular NAMPT (visfatin) to HCT116 p53 WT cells depleted of NAMPT. Figure S8. (A) Clonal heterogeneity analysis reveals that 2.5 nM FK866 abruptly decreases the number of holoclones in both cell lines. (B) Clonal heterogeneity analysis reveals that 1 ÂµM sirtinol also abruptly decreases the number of holoclones. Figure S8C-I. Effect of the addition of the Sirt1 inhibitor Sirtinol to HCT116 p53 WT cells overexpressing NAMPT. Left half of the figure: we added 1 ÂµM sirtinol to cells overexpressing NAMPT (NAMPT+S, Â-�, orange bars) and compared different properties to parental expressing only vector (v, Â-�, grey bars), shRNA only (sh, O, white bars) or NAMPT overexpression only (NAMPT, Â- , black bars). (C) growth curve, (D) clonogenic assay, (E) CD133 percentage of cells, (F) number of tumorspheres, (G) Colony type, (H) stem cell pathway effectors and (I) EMT effectors [*, p<0.05; **, p<0.01; ***, p<0.001 compared to NAMPT overexpression only]. Figure S9. Effect of the addition of the NAMPT inhibitor (FK866) to HCT116 p53 WT cells overexpressing NAMPT. Table S1. Correlation analysis using GSE20916 of the prognosis predictive gene set driven by NAMPT. Here, we show correlations of each element with NAMPT. All data shown is highly significant (p<0.01).</p>
Manganese‐Based Antioxidase‐Inspired Biocatalysts with Axial Mn−N5Sites and 2D d‐π‐Conjugated Networks for Rescuing Stem Cell Fate
Authors: Wu Zihe , Sun Yimin , Mu Shengdong , Bai Mingru , Li Qian , Ma Tian , Ma Lang , Chen Fan , Luo Xianglin , Ye Ling , Cheng Chong 
Published: April 2023

AbstractConstructing highly effective biocatalysts with controllable coordination geometry for eliminating reactive oxygen species (ROS) to address the current bottlenecks in stem‐cell‐based therapeutics remains challenging. Herein, inspired by the coordination structure of manganese‐based antioxidase, we report a manganese‐coordinated polyphthalocyanine‐based biocatalyst (Mn−PcBC) with axial Mn−N5sites and 2D d‐π‐conjugated networks that serves as an artificial antioxidase to rescue stem cell fate. Owing to the unique chemical and electronic structures, Mn−PcBC displays efficient, multifaceted, and robust ROS‐scavenging activities, including elimination of H2O2and O2⋅−. Consequently, Mn−PcBC efficiently rescues the bioactivity and functionality of stem cells in high‐ROS‐level microenvironments by protecting the transcription of osteogenesis‐related genes. This study offers essential insight into the crucial functions of axially coordinated Mn−N5sites in ROS scavenging and suggests new strategies to create efficient artificial antioxidases for stem‐cell therapies.
2‐Phenylquinazolin‐4(3H)‐one scaffold as newly designed, synthesized VEGFR‐2 allosteric inhibitors with potent cytotoxicity through apoptosis
Authors: Elrayess Ranza , Elgawish Mohamed S. , Nafie Mohamed S. , Ghareb Nagat , Yassen Asmaa S. A. 
Published: April 2023

AbstractNew derivatives of 2‐phenylquinazolin‐4(3H)‐one were designed, synthesized, and biologically evaluated as potent allosteric kinase inhibitors with in situ cytotoxicity against MCF‐7 and HepG2 cells. Compounds 15 and 18 among the proposed compounds showed promising antiproliferative activity against MCF‐7 (IC50 = 1.35 µM) and HepG2 cells (IC50 = 3.24 µM), comparable to sorafenib, with IC50 values of 3.04, 2.93 µM, respectively, according to in situ cytotoxicity testing. Comparing compounds 15 and 18 to sorafenib, the in vitro VEGFR‐2 inhibitory activity displayed encouraging selective efficacy with IC50 values of 13, 67, and 30 nM, respectively. Results of VEGFR‐2 inhibition at various ATP concentrations proved that there was no statistically significant difference between the IC50 values, which improved the non‐ATP competitive binding. Compound 15 caused apoptotic breast cancer cell death with 55.11‐fold cell‐cycle arrest at the S‐phase, where it affected the apoptosis‐mediated genes through upregulating P53, Bax, caspases 3, 8, and 9 and downregulating the antiapoptotic gene Bcl‐2. A molecular docking study was conducted to confirm the binding of the designed compounds to the allosteric site of VEGFR‐2 in DFG‐out mode, leaving the ATP‐binding pocket unoccupied when superimposed to the pose of sorafenib. The designed molecules showed resealable binding affinity toward the DFG loop and the allosteric site. Hence, the 2‐phenylquinazolin‐4(3H)‐one derivative constitutes intriguing starting points for designing apoptotic‐inducing drugs.
Quantification of cefaclor in human plasma using SIL‐IS LC‐ESI‐MS/MS for pharmacokinetics study in healthy Chinese volunteers
Authors: Chen Xinyan , Liu Gege , Wang Changmao , Liu Ran , Wang Minhui , Huang Yunzhe , Shen Jie , Jia Yuanwei 
Published: April 2023

AbstractA steady, high‐efficiency, and precise liquid chromatography‐electrospray ionization‐tandem mass spectrometry method was established and validated using cefaclor‐d5 as the stable isotope‐labeled internal standard for quantification of cefaclor in human plasma. One‐step protein precipitation was applied to extract human plasma samples using methanol as precipitant. An Ultimate XB C18 column (2.1 × 50.0 mm, 5.0 μm) was used to achieve chromatographic separation. Mobile phases of gradient elution were an aqueous solution containing 0.1% formic acid (mobile phase A) and an acetonitrile solution containing 0.1% formic acid (mobile phase B). Electrospray ionization in positive‐ion mode was applied to detect under multiple reaction monitoring mode. Target fragment ion pairs of cefaclor and stable isotope‐labeled internal standard, respectively, were m/z 368.2 → 191.1 and m/z 373.2 → 196.1. Linear range of this method was between 20.0 and 10,000.0 ng/ml, with coefficient of determination (R2) >0.9900. Seven concentrations of quality control samples were used: 20.0 ng/ml (lower limit of quantitation), 60.0 ng/ml (low quality control), 650 ng/ml (middle quality control), 5000 ng/ml (arithmetic average middle quality control [AMQC]), 7500 ng/ml (high quality control), 10,000 ng/ml (upper limit of quantification), and 40,000 ng/ml (dilution quality control [DQC]). The method was validated for selectivity, lower limit of quantitation, linearity, accuracy, precision, recovery, matrix effect, dilution reliability, stability, carryover, and incurred sample reanalysis. This stable isotope‐labeled internal standard liquid chromatography‐electrospray ionization‐tandem mass spectrometry approach has been successfully applied to study the pharmacokinetics of cefaclor dry suspension among healthy Chinese volunteers.
Randomized clinical trial on the effect of intermittent vibrational force application during orthodontic treatment with aligners on RANKL and OPG concentrations in crevicular fluid
Authors: Pérez Idarraga Alicia , Yeste Ojeda Fara , Virto Ruiz Leire , Lacasa Litner Miguel , Cacho Casado Alberto , Martin Conchita 
Published: April 2023

AbstractApplication of intermittent forces by vibration is proposed as an easy‐to‐use accelerator of dental movement. The purpose of this study was to determine the effect of intermittent vibrational force application during orthodontic aligner treatment on receptor activator of nuclear factor‐kappa B ligand (RANKL) and osteoprotegerin (OPG) concentrations in crevicular fluid as markers of bone remodeling. This three‐arm parallel randomized clinical trial included 45 candidates for malocclusion treatment with aligners, randomly assigned to: Group A (vibrational forces from onset of treatment); Group B (vibrational forces at 6 weeks after treatment onset); or Group C (no vibration). The frequency of aligner adjustment also differed among groups. At different time points, a paper tip was used to draw crevicular fluid samples from a moving lower incisor for RANKL and OPG analysis using ELISA kits. Mixed‐model ANOVA found no significant differences in RANKL (A: p = 0.31, B: p = 0.8, C: p = 0.49) or OPG (A: p = 0.24, B: p = 0.58, C: p = 0.59) over time in any group or as a function of the application/non‐application of vibration or the frequency of aligner adjustments. Application of this accelerator device did not significantly affect bone remodeling in patients undergoing orthodontic treatment with aligners. However, a nonsignificant improvement in biomarker concentrations was observed when aligners were changed every 7 days and vibration was also applied. Further research is warranted to establish protocols for the application of vibration and the timing of aligner adjustments.
Management of failed stenting of the unprotected left main coronary artery
Authors: Moroni Alice , Marin Federico , Venturi Gabriele , Scarsini Roberto , Ribichini Flavio , De Maria Giovanni Luigi , Banning Adrian P. 
Published: April 2023

AbstractPercutaneous coronary intervention (PCI) is increasingly accepted as treatment for unprotected left main coronary artery (ULMCA) disease especially in those patients who are unsuitable for cardiac surgery. Treatment of any stent failure is associated with increased complexity and worse clinical outcomes when compared with de novo lesion revascularization. Intracoronary imaging has provided new insight into mechanisms of stent failure and treatment options have developed considerably over the last decade. There is paucity of evidence on the management strategy for stent failure in the specific setting of ULMCA. Treating any left main with PCI requires careful consideration and consequently treatment of failed stents in ULMCA is complex and provides unique challenges. Consequently, we provide an overview of ULMCA stent failure, proposing a tailored algorithm to guide best management and decision in daily clinical practice, with a special focus on intracoronary imaging characterization of causal mechanisms and specific technical and procedural considerations.
Synthesis and Anti‐Mycobacterium tuberculosis Activity of Imidazo[2,1‐b][1,3]oxazine Derivatives against Multidrug‐Resistant Strains
Authors: Fernandes Guilherme F. S. , Manieri Karyn F. , Bonjorno Andressa F. , Campos Debora L. , Ribeiro Camila M. , Demarqui Fernanda M. , Ruiz Daniel A. G. , Nascimento‐Junior Nailton M. , Denny William A. , Thompson Andrew M. , Pavan Fernando R. , Dos Santos Jean L. 
Published: April 2023

AbstractThe emergence of multidrug‐resistant strains of M. tuberculosis has raised concerns due to the greater difficulties in patient treatment and higher mortality rates. Herein, we revisited the 2‐nitro‐6,7‐dihydro‐5H‐imidazo[2,1‐b][1,3]oxazine scaffold and identified potent new carbamate derivatives having MIC90 values of 0.18–1.63 μM against Mtb H37Rv. Compounds 47–49, 51–53, and 55 exhibited remarkable activity against a panel of clinical isolates, displaying MIC90 values below 0.5 μM. In Mtb‐infected macrophages, several compounds demonstrated a 1‐log greater reduction in mycobacterial burden than rifampicin and pretomanid. The compounds tested did not exhibit significant cytotoxicity against three cell lines or any toxicity to Galleria mellonella. Furthermore, the imidazo[2,1‐b][1,3]oxazine derivatives did not show substantial activity against other bacteria or fungi. Finally, molecular docking studies revealed that the new compounds could interact with the deazaflavin‐dependent nitroreductase (Ddn) in a similar manner to pretomanid. Collectively, our findings highlight the chemical universe of imidazo[2,1‐b][1,3]oxazines and their promising potential against MDR‐TB.
Drop coating deposition Raman (DCDR) spectroscopy of biologically important molecules
Authors: Kuižová Alžbeta , Kočišová Eva 
Published: April 2023

AbstractDrop coating deposition Raman (DCDR) spectroscopy introduces a simple and easily accessible approach to studying biologically important molecules and their mixtures. The method is based on drying a small drop of solution or suspension of studied molecules deposited on a special hydrophobic surface. The drying process efficiently accumulates the molecules in the ‘coffee‐ring’ or any small pattern from which the Raman spectrum can be measured. In this way, a significant (several orders of magnitude) improvement of the Raman detection sensitivity compared with Raman measurement from solution can be reached. Therefore, a small sample volume (several microliters), as well as a low initial concentration of studied molecules in deposited droplets, are important advantages of the DCDR method over the normal Raman one. Recently, many relevant DCDR applications on biomolecules and related molecules have been reported. The mini‐review covers a brief overview of the DCDR method (principle, short history, suitable hydrophobic surfaces and state‐of‐the‐art). It will be followed by a summary of the studies over the last 15–20 years on different biologically important molecules, including proteins, lipids (in the form of liposomes) and small molecules (e.g., porphyrins, anthrax marker dipicolinic acid and food and environmental contaminants). Finally, the application potential and further perspectives of the DCDR method for biomolecular studies will be discussed.
Synthesis of Multifunctional Protein‐Polymer Conjugates via Oxygen‐tolerant, Aqueous Copper‐Mediated Polymerization, and Bioorthogonal Click Chemistry
Authors: Voutyritsa Errika , Gryparis Charis , Theodorou Alexis , Velonia Kelly 
Published: April 2023

AbstractOxygen‐tolerant, aqueous copper‐mediated polymerization approaches are combined with click chemistry in either a sequential or a simultaneous manner, to enable the synthesis of multifunctional protein‐polymer conjugates. Propargyl acrylate (PgA) and propargyl methacrylate (PgMA) grafting from a bovine serum albumin (BSA) macroinitiator is thoroughly optimized to synthesize chemically addressable BSA‐poly(propargyl acrylate) and BSA‐poly(propargyl methacrylate) respectively. The produced multifunctional bioconjugates bear pendant terminal 1‐alkynes which can be readily post‐functionalized via both [3+2] Huisgen cycloaddition and thiol‐yne click chemistry under mild reaction conditions. Simultaneous oxygen‐tolerant, aqueous copper‐catalyzed polymerization, and click chemistry mediate the in situ multiple chemical tailoring of biomacromolecules in excellent yields.
Renewing the resource‐based view: New contexts, new concepts, and new methods
Authors: Helfat Constance E. , Kaul Aseem , Ketchen David J. , Barney Jay B. , Chatain Olivier , Singh Harbir 
Published: April 2023

AbstractThe resource‐based view is an enduring and impactful mainstay of research within strategic management and beyond. This editors' introduction to the special issue on “new directions for the resource‐based view” accomplishes two main tasks. First, we describe the contributions offered by the seven articles contained in the special issue. Second, we explain the potential value to research of incorporating into resource‐based inquiry new contexts (artificial intelligence and digitization, distributed organizations, and stakeholders and sustainability); new concepts (resource redeployment, market shaping through resources and capabilities); and new methods (text analysis and machine learning, formal models, policy capturing). The overall aim of this introduction is to help invigorate the resource‐based view by spotlighting a series of promising new directions.
Effect of flowing water on the pharmacokinetic properties of norfloxacin in channel catfish (Ictalurus punctatus) after single‐dose oral administration
Authors: Yu Yali , Dong Lixue , Zhang Lang , Gan Jinhua , Peng Jie , Liu Ting , Chen Jianwu , Lu Xiaorong , He Li , Cheng Bo 
Published: April 2023

AbstractBackgroundThe recirculating aquaculture system (RAS) is a widely used, water‐saving and efficient aquaculture model. However, bacterial diseases are common in farmed fish reared at high densities. Although antibiotics effectively treat these diseases, developing efficient methods to increase drug clearance in fish and decrease the concentrations of antibiotic residues in aquatic products is essential.ObjectivesThis study evaluates the effect of flowing water in the RAS on norfloxacin (NOR) pharmacokinetics in channel catfish (Ictalurus punctatus).MethodsChannel catfish were randomly divided into the control group (RAS group) and the experimental group (flow‐through aquaculture system group) (120 individuals/group). A NOR dose of 20 mg/kg was then orally administered to the fish. Plasma, muscle, liver and kidney samples were collected up to 168 h after treatment. NOR concentrations were measured using liquid chromatography–mass spectrometry, and pharmacokinetic parameters were calculated using a non‐compartmental method.ResultsFlowing water had a significant effect on the plasma pharmacokinetics and tissue distribution of NOR, increasing NOR clearance in the kidney, muscle and plasma. The time to maximum concentration of NOR was shorter in the plasma and longer in the kidney and liver. Moreover, flowing water increased the maximum concentration of NOR in the kidney, muscle and plasma and decreased the area under the concentration–time curve from time 0 to the last measurable concentration in the liver and plasma. Flowing water decreased the withdrawal period in muscle from 10 to 6 days.ConclusionsThese results indicate that flowing water can potentially increase NOR clearance in channel catfish.
Expression of SATB1 and SATB2 in the brain of bony fishes: what fish reveal about evolution
Authors: Lozano Daniel , López Jesús M. , Jiménez Sara , Morona Ruth , Ruíz Víctor , Martínez Ana , Moreno Nerea 
Published: April 2023

AbstractSatb1 and Satb2 belong to a family of homeodomain proteins with highly conserved functional and regulatory mechanisms and posttranslational modifications in evolution. However, although their distribution in the mouse brain has been analyzed, few data exist in other non-mammalian vertebrates. In the present study, we have analyzed in detail the sequence of SATB1 and SATB2 proteins and the immunolocalization of both, in combination with additional neuronal markers of highly conserved populations, in the brain of adult specimens of different bony fish models at key evolutionary points of vertebrate diversification, in particular including representative species of sarcopterygian and actinopterygian fishes. We observed a striking absence of both proteins in the pallial region of actinopterygians, only detected in lungfish, the only sarcopterygian fish. In the subpallium, including the amygdaloid complex, or comparable structures, we identified that the detected expressions of SATB1 and SATB2 have similar topologies in the studied models. In the caudal telencephalon, all models showed significant expression of SATB1 and SATB2 in the preoptic area, including the acroterminal domain of this region, where the cells were also dopaminergic. In the alar hypothalamus, all models showed SATB2 but not SATB1 in the subparaventricular area, whereas in the basal hypothalamus the cladistian species and the lungfish presented a SATB1 immunoreactive population in the tuberal hypothalamus, also labeled with SATB2 in the latter and colocalizing with the gen Orthopedia. In the diencephalon, all models, except the teleost fish, showed SATB1 in the prethalamus, thalamus and pretectum, whereas only lungfish showed also SATB2 in prethalamus and thalamus. At the midbrain level of actinopterygian fish, the optic tectum, the torus semicircularis and the tegmentum harbored populations of SATB1 cells, whereas lungfish housed SATB2 only in the torus and tegmentum. Similarly, the SATB1 expression in the rhombencephalic central gray and reticular formation was a common feature. The presence of SATB1 in the solitary tract nucleus is a peculiar feature only observed in non-teleost actinopterygian fishes. At these levels, none of the detected populations were catecholaminergic or serotonergic. In conclusion, the protein sequence analysis revealed a high degree of conservation of both proteins, especially in the functional domains, whereas the neuroanatomical pattern of SATB1 and SATB2 revealed significant differences between sarcopterygians and actinopterygians, and these divergences may be related to the different functional involvement of both in the acquisition of various neural phenotypes.
Renal cryoablation combined with prior transcatheter arterial embolization in non-dialysis patients with stage 4 or 5 chronic kidney disease: a retrospective study
Authors: Umakoshi Noriyuki , Iguchi Toshihiro , Matsui Yusuke , Tomita Koji , Uka Mayu , Kawabata Takahiro , Munetomo Kazuaki , Nagata Shoma , Gobara Hideo , Araki Motoo , Hiraki Takao 
Published: April 2023

Abstract
Purpose
To retrospectively evaluate cryoablation combined with prior transcatheter arterial embolization (TAE) for renal cell carcinoma (RCC) in non-dialysis patients with stage 4 or 5 chronic kidney disease (CKD).

Materials and methods
Patients with stage 4 or 5 CKD undergoing TAE and cryoablation for RCC between May 2012 and October 2021 were included. TAE was selectively performed using iodized oil with absolute ethanol or gelatin sponge 1–14 days before cryoablation. Local efficacy, safety, and changes in renal function were evaluated.

Results
Nine patients (seven men and two women; median age, 64 years; range 52–88 years) with nine RCCs (mean diameter, 3.0 ± 1.0 cm; range 1.7–4.7 cm) were included. The mean pre-treatment estimated glomerular filtration rate (eGFR) was 24.2 ± 5.6 ml/min/1.73 m2 (range 10.4–29.2 ml/min/1.73 m2). The mean amount of contrast medium used in TAE was 58 ± 29 ml (range 40–128 ml). Except in one patient (grade 3 pyelonephritis), no grade ≥ 3 complications occurred. During the follow-up period (median, 18 months; range 7–54 months), no local tumor progression occurred. In two patients with pre-treatment eGFR of < 20 ml/min/1.73 m2, hemodialysis was initiated at 3 and 19 months after cryoablation. At their last follow-up, the remaining seven patients showed a decrease of 6.2 ± 5.3 ml/min/1.73 m2 (range 0.7–17.2 ml/min/1.73 m2) in their eGFR.

Conclusion
Cryoablation combined with TAE for RCC in non-dialysis patients with stage 4 or 5 CKD was effective and safe, with an acceptable impact on renal function.