bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient Natural Plasmid Transformation ofVibrio natriegensEnables Zero-capital Molecular Biology
Authors: Specht David A. , Sheppard Timothy J. , Kennedy Finn , Li Sijin , Gadikota Greeshma , Barstow Buz 
Published: August 2023

AbstractThe fast-growing microbeVibrio natriegensis capable of natural transformation where it draws DNA in from media via an active process under physiological conditions. Using an engineered strain with a genomic copy of the master competence regulatortfoXfromVibrio cholerain combination with a new minimal competence media (MCM) that uses acetate as an energy source, we demonstrate naturally competent cells which are created, transformed, and recovered entirely in the same media, without exchange or addition of new media. Cells are naturally competent to plasmids, recombination with linear DNA, and co-transformation of both to select for scarless and markerless genomic edits. The entire process is simple and inexpensive, requiring no capital equipment for an entirely room temperature process (Zero Capital protocol, 104cfu/µg), or just an incubator (High Efficiency protocol, 105–6cfu/µg). These cells retain their naturally competent state when frozen and are transformable immediately upon thawing like a typical chemical or electrochemical competent cell. Since the optimized transformation protocol requires only 50 minutes of hands-on time, andV. natriegensgrows quickly even on plates, a transformation started at 9 AM yields abundant culturable single colonies by 5 PM. Further, because all stages of transformation occur in the same media, and the process can be arbitrarily scaled in volume, this natural competence strain and media could be ideal for automated directed evolution applications. As a result, naturally competentV. natriegenscould compete withE. colias an excellent chassis for low-cost and highly scalable synthetic biology.
A fluorescent sex-sorting technique for insects with the demonstration inDrosophila melanogaster
Authors: Liu Junru , Rayes Danny , Akbari Omar S. 
Published: August 2023

AbstractRecent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here we describe the development of a sex-sorting technique for female and male selection with a proof-of-concept inD. melanogastertermed SEPARATOR (SexingElementProduced byAlternativeRNA-splicing ofATransgenicObservableReporter). This approach utilizes dominant fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-sorting.
Mammalian olfactory cortex neurons retain molecular signatures of ancestral cell types
Authors: Zeppilli S. , Gurrola A. Ortega , Demetci P. , Brann D. H. , Attey R. , Zilkha N. , Kimchi T. , Datta S. R. , Singh R. , Tosches M. A. , Crombach A. , Fleischmann A. 
Published: August 2023

AbstractThe cerebral cortex diversified extensively during vertebrate evolution. Intriguingly, the three-layered mammalian olfactory cortex resembles the cortical cytoarchitecture of non-mammals yet evolved alongside the six-layered neocortex, enabling unique comparisons for investigating cortical neuron diversification. We performed single-nucleus multiome sequencing across mouse three- to six-layered cortices and compared neuron types across mice, reptiles and salamander. We identified neurons that are olfactory cortex-specific or conserved across mouse cortical areas. However, transcriptomically similar neurons exhibited area-specific epigenetic states. Additionally, the olfactory cortex showed transcriptomic divergence between lab and wild-derived mice, suggesting enhanced circuit plasticity through adult immature neurons. Finally, olfactory cortex neurons displayed marked transcriptomic similarities to reptile and salamander neurons. Together, these data indicate that the mammalian olfactory cortex retains molecular signatures representative of ancestral cortical traits.
Ascending vaginal infection in mice induces preterm birth and neonatal morbidity
Authors: Boyle Ashley K , Tetorou Konstantina , Suff Natalie , Beecroft Laura , Mazzaschi Margherita , Hristova Mariya , Waddington Simon N , Peebles Donald 
Published: August 2023

AbstractPreterm birth (PTB; delivery <37 weeks), the main cause of neonatal death worldwide, can lead to adverse neurodevelopmental outcomes, as well as lung and gut pathology. PTB is commonly associated with ascending vaginal infection. Previously, we have shown that ascendingE. coliinfection in pregnant mice induces PTB and reduces pup survival. Here, we demonstrate that this model recapitulates the pathology observed in human preterm neonates, namely neuroinflammation, lung injury and gut inflammation. In neonatal brains, there is widespread cell death, microglial activation, astrogliosis and reduced neuronal density. We also validate the utility of this model by assessing efficacy of maternal cervical gene therapy with an adeno-associated viral vector containing human beta defensin 3; this improves pup survival and reducesTnfαmRNA expression in perinatal pup brains exposed toE. coli. This model provides a unique opportunity to evaluate the therapeutic benefit of preterm labour interventions on perinatal pathology.
Sustainable consumer choices – critical reflection on hospitality and tourism
Authors: Sharma Amit , Saulais Laure , Huang Yidan 
Published: August 2023

Purpose
Strategies to promote more sustainable consumer choices have been gaining interest among tourism and hospitality scholars. In particular, behavioral economic theories of decision-making have gained popularity in the past decade, led by behavioral interventions (BIs) such as the nudge movement. This paper aims to present a critical reflection on this recent trend, with a specific focus on whether these BI approaches are an adequate tool to contribute to long-term behavioral changes, one crucial aim of the promotion of sustainable consumption.

Design/methodology/approach
Based on a critical review of recent significant academic works in the field, this paper reflects on how nudge principles are applied in the hospitality and tourism sectors, as well as the usual justifications given for their use. This paper then discusses the potential limitations, both theoretical and practical, of using these short-term focused approaches to decisions that intend to have long-term outcomes and aims.

Findings
BIs in hospitality and tourism have the potential to create long-term sustainable changes through a more comprehensive view of behavioral factors influencing decisions; however, such approaches would need to be strongly embedded in theoretical arguments that question “how” and “why” behavior change could be sustainable in the long term. This paper proposes a conceptual framework to address these concerns for future research.

Research limitations/implications
This critical reflection proposes a comprehensive framework that will help guide stronger theoretically motivated identification, design and empirical testing of BIs and nudges. Industry can eventually benefit from theoretically stronger interventions that provide a balance between the short-term and long-term influence of BIs to attain customer loyalty and eventually greater value for business stakeholders.

Originality/value
This reflection paper critically reviews the basis of BIs and recommends a framework to strengthen their theoretical arguments. This reflection focuses on the theoretical critique of BIs and nudges to ensure long-term behavior changes are sustainable. The paper also proposes a comprehensive framework that incorporates well-founded theoretical models to enhance BIs and nudge literature.
EMAS III-based analysis of European eco-management for energy efficiency investments
Authors: Dinçer Hasan , Yuksel Serhat , Bhatti Muhammad Ishaq M. , Mikhaylov Alexey 
Published: August 2023

PurposeThe aim is to analyze the European eco-management because the global warming has become a topical issue impacting the whole world. Individual countries are trying to minimize all the catalysts of global warming, such as carbon emissions. This paper addresses this issue and analyzes the performance of European eco-management for the purpose of future energy investments being environmentally.Design/methodology/approachThis paper develops a fuzzy decision-making model to study the performance indicators of selected countries based on EMAS III standard. It employs interval type-2 fuzzy DEMATEL to evaluate the performance factors and TOPSIS methodology to assess five selected European countries' performance in relation to eco-friendly, emission and renewable energy.FindingsEco-friendly energy plays the most critical role in this respect followed by emissions and renewable energy which constitute significant factors. The novelty of this study is identifying significant criteria regarding environmental and energy efficiency of investments and making performance assessments of European countries with a new fuzzy decision-making model. Both expert opinions and datasets are used for the analysis. This paper supports previous research about energy efficiency investments in Europe.Research limitations/implicationsThe innovative feature of this study is identifying significant criteria regarding environmental and energy efficiency of investments and assessing the performance of European countries with a new fuzzy decision-making model. The fact that the analysis only concerns the European region is an important limitation. In future analyses, other groups of countries can be examined. Innovations can be made regarding the method applied. In this context, analyses can be done utilizing different fuzzy numbers. Finally, the importance of the criteria can be calculated with other methods such as SWARA.Practical implicationsThe paper fills the gap in performance analysis of European eco-management for environmentally friendly and efficient energy investments is done in this manuscript.Originality/valueAnalysis of European eco-management performance was done for environmentally friendly and efficient energy investments. A fuzzy decision-making model is constructed. The paper fills the gap in performance analysis of European eco-management for environmentally friendly and efficient energy investments.
Using health belief model and social media analytics to develop insights from hospital-generated twitter messaging and community responses on the COVID-19 pandemic
Authors: Tian Xin , He Wu , He Yuming , Albert Steve , Howard Michael 
Published: August 2023

PurposeThis study aims to examine how different hospitals utilize social media to communicate risk information about COVID-19 with the communities they serve, and how hospitals' social media messaging (firm-generated content and their local community's responses (user-generated content) evolved with the COVID-19 outbreak progression.Design/methodology/approachThis research proposes a healthcare-specific social media analytics framework and studied 68,136 tweets posted from November 2019 to November 2020 from a geographically diverse set of ten leading hospitals' social media messaging on COVID-19 and the public responses by using social media analytics techniques and the health belief model (HBM).FindingsThe study found correlations between some of the HBM variables and COVID-19 outbreak progression. The findings provide actionable insight for hospitals regarding risk communication, decision making, pandemic awareness and education campaigns and social media messaging strategy during a pandemic and help the public to be more prepared for information seeking in the case of future pandemics.Practical implicationsFor hospitals, the results provide valuable insights for risk communication practitioners and inform the way hospitals or health agencies manage crisis communication during the pandemic For patients and local community members, they are recommended to check out local hospital's social media sites for updates and advice.Originality/valueThe study demonstrates the role of social media analytics and health behavior models, such as the HBM, in identifying important and useful data and knowledge for public health risk communication, emergency responses and planning during a pandemic.
Media presentation of hospital discharge to care homes during the COVID-19 pandemic
Authors: Abe Eniola , Dawson Pamela , Scott Jason 
Published: August 2023

PurposeAt the outset of the COVID-19 pandemic the United Kingdom Government implemented a policy to rapid discharge hospital patients into care homes. This study aimed to examine how the media in the United Kingdom portrayed hospital discharge to care homes during the COVID-19 pandemic.Design/methodology/approachThis study was a qualitative document analysis. Four sources (Daily Mail,The Independent,The GuardianandBBC News) were selected to represent political orientations encompassing right-wing, centrist and left-wing perspectives, and were searched for mention of hospital discharge, care homes and Covid-19 pandemic between 1st January 2020 and 24th February 2022. Article text was copied verbatim into Microsoft Word documents prior to analysis. Data were thematically analysed, followed by coding the sentiment in the included articles as well as coding the sentiment of themes and sub-themes.FindingsOf 722 identified articles, 133 were eligible for inclusion as the final corpus. Data represented a moralistic narrative consisting of four themes: (1) Government as villain, (2) care homes as antiheroes, (3) patients as ideal victims and (4) moral outcomes. Most of the corpus had a negative sentiment (78.1%). One theme, moral outcomes, had considerably more positive sentiment (32.4%) than others (range 15.1%–21.9%).Originality/valueA moralistic argument for improving cross-boundary interactions between health and social care services is provided, and the media can play a role pushing cross-boundary working higher up the policy agenda. Future work should examine how direct stakeholders, including those working in healthcare and care home settings, perceived the discharge policy.
Inventory management and TQM practices for better firm performance: a systematic and bibliometric review
Authors: Mahajan Pramod Sanjay , Raut Rakesh D. , Kumar Prasanth R. , Singh Vikas 
Published: August 2023

PurposeThis paper aims to quantify the trend of variables used for building a theoretical model with the help of analysing bibliometrics data of inventory management (IM) and Total Quality Management (TQM) practices and their effects on firm performance.Design/methodology/approachScopus' research database and the Web of Science (WoS) (including Elsevier, Emerald Group Publishing, Taylor and Francis, Wiley, IEEE, Informs and SAGE) were used to find relevant articles. The articles, review papers and conference proceedings were screened from 1993 to 2021. The articles were analysed to explain the different types of IM practices, TQM practices and their effect on the firm's performance. Thematic analysis was done using a bibliometric package of “R” (Biblioshiny) and VOSviewer to identify the key trends, approaches and research agenda.FindingsThe research covered 28 years of publications and summarised 497 articles, review papers and conference papers. Researchers concluded that IM highly correlates with the inventory turnover ratio and has no relation to firm performance. Further, TQM positively affects firm performance, but integrating IM and TQM will be a research scope for future study as none of the researchers previously covered this.Research limitations/implicationsDue to the systematic literature review (SLR) and Bibliometric review, the study is limited to the Scopus and WoS (including Elsevier, Emerald Group Publishing, Taylor and Francis, Wiley, IEEE, Informs and SAGE) databases.Originality/valueBeing the research on a bibliometric and systematic review, relating IM practices with TQM practices would be the novelty of this paper.
A multilocus sequence typing method of Staphylococcus aureus DNAs in a sample from human skin
Authors: Furuya Hiroka , Ogura Kohei , Takemoto Norihiko , Watanabe Shinya , Yamazaki Ayaka , Ogai Kazuhiro , Sugama Junko , Okamoto Shigefumi 
Published: August 2023

AbstractThe skin and mucous membranes are the primary sites of Staphylococcus aureus colonization, particularly those of health care personnel and patients in long‐term care centers. We found that S. aureus colonized with a higher abundance ratio on skins which had recovered from pressure injury (PI) than on normal skins in our earlier research on the skin microbiota of bedridden patients. Multilocus sequence typing (MLST) is a useful tool for typing S. aureus isolated from clinical specimens. However, the MLST approach cannot be used in microbiota DNA owing to the contamination from other bacteria species. In this study, we developed a multiplex‐nested PCR method to determine S. aureus MLST in samples collected from human skins. The seven pairs of forward and reverse primers were designed in the upstream and downstream regions, which were conserved specifically in S. aureus. The first amplifications of the seven pairs were conducted in a multiplex assay. The samples were diluted and applied to conventional PCR for MLST. We confirmed that the method amplified the seven allele sequences of S. aureus specifically in the presence of untargeted DNAs from human and other skin commensal bacteria. Using this assay, we succeeded in typing sequence types (STs) of S. aureus in the DNA samples derived from the skins healed from PI. Peaks obtained by Sanger sequencing showed that each sample contained one ST, which were mainly categorized into clonal complex 1 (CC1) or CC5. We propose that this culture‐free approach may be used in detecting S. aureus in clinical specimens without isolation.
Increased clonal growth in heavily harvested ecosystems failed to rescue ayahuasca lianas from decline in the Peruvian Amazon rainforest
Authors: Coe Michael A. , Gaoue Orou G. 
Published: August 2023

Abstract

Increasing harvest and overexploitation of wild plants for non‐timber forest products can significantly affect population dynamics of harvested populations. While the most common approach to assess the effect of harvest and perturbation of vital rates is focused on the long‐term population growth rate, most management strategies are planned and implemented over the short‐term.

We developed an integral projection model to investigate the effects of harvest on the demography and the short‐ and long‐term population dynamics of Banisteriopsis caapi in the Peruvian Amazon rainforest.

Harvest had no significant effect on the size‐dependent growth of lianas, but survival rates increased with size. Harvest had a significant negative effect on size‐dependent survival where larger lianas experienced greater mortality rates under high harvest pressure than smaller lianas. In the populations under high harvest pressure, survival of smaller lianas was greater than that of populations with low harvest pressure. Harvest had no significant effect on clonal or sexual reproduction, but fertility was size‐dependent.

The long‐term population growth rates of B. caapi populations under high harvest pressure were projected to decline at a rate of 1.3% whereas populations with low harvest pressure are expected to increase at 3.2%. However, before reaching equilibrium, over the short‐term, all B. caapi populations were in decline by 26% (high harvested population) and (low harvested population) 20.4% per year.

Elasticity patterns were dominated by survival of larger lianas irrespective of harvest treatments. Life table response experiment analyses indicated that high harvest caused the 6% reduction in population growth rates by significantly reducing the survival of large lianas and increasing the survival‐growth of smaller lianas including vegetative reproductive individuals.

Synthesis and applications. This study emphasizes how important it is for management strategies for B. caapi lianas experiencing anthropogenic harvest to prioritize the survival of larger size lianas and vegetative reproducing individuals, particularly in increased harvested systems often prone to multiple stressors. From an applied conservation perspective, our findings illustrate the importance of both prospective and retrospective perturbation analyses in population growth rates in understanding the population dynamics of lianas in general in response to human‐induced disturbance.
Ligustrazine and liguzinediol protect against doxorubicin‐induced cardiomyocytes injury by inhibiting mitochondrial apoptosis and autophagy
Authors: Lian Naqi , Tong Jing , Zhu Weijie , Meng Qinghai , Jiang Miao , Bian Mianli , Li Yu 
Published: August 2023

AbstractPreventing or treating heart failure (HF) by blocking cardiomyocyte apoptosis is an effective strategy that improves survival and reduces ventricular remodelling and dysfunction in the chronic stage. Autophagy is a mechanism that degrades intracellular components and compensates for energy deficiency, which is commonly observed in cardiomyocytes of failed hearts. Cardiomyocytes activated by doxorubicin (DOX) exhibit strong autophagy. This study aims to investigate the potential protective effect of ligustrazine and its derivative liguzinediol on regulating DOX‐induced cardiomyocyte apoptosis and explore the use of the embryonic rat heart‐derived myoblast cell line H9C2 for identifying novel treatments for HF. The results indicated that it has been demonstrated to reverse myocardial infarction remodelling in failed hearts by promoting autophagy in salvaged cardiomyocytes and anti‐apoptosis of cardiomyocytes in granulation tissue. Our study suggests that ligustrazine and liguzinediol can be a promising agents and autophagy is potential pathway in the management of HF.
Spillover effects of FDI inflows on output growth: An analysis of aggregate and disaggregated FDI inflows of 13 MENA economies
Authors: Bergougui Brahim , Murshed Syed Mansoob 
Published: August 2023

AbstractThis paper examines the effect of foreign direct investment (FDI) inflows on overall growth, as well as its sector‐specific spillovers in the Middle Eastern and North African region during the period from 2000 to 2020. Our major innovation is our ability to disaggregate FDI into primary, secondary and tertiary and examine their individual impact on growth, as well as their sector‐specific spillovers by using dynamic panel GMM methodology. We find prima facie evidence that total FDI significantly stimulates growth. However, when we turn to the disaggregated FDI data, primary sector FDI adversely affects the gross domestic product (GDP) growth in the service sector and overall GDP growth. On the other hand, secondary FDI has a ‘double‐edged’ effect, benefiting its own sector (the service sector's GDP growth), but not other sectors. In contrast, service sector FDI stimulates GDP growth in mining, manufacturing and service sectors, thereby enhancing overall economic growth. Our findings have important policy implications regarding the incentives provided by governments to encourage FDI, which need to be fine‐tuned to attract certain types of FDI (tertiary), with less focus on the primary sector