bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEXEL is a proteolytic maturation site for both exported and non-exportedPlasmodiumproteins
Authors: Fierro Manuel A , Muheljic Ajla , Sha Jihui , Wohlschlegel James A , Beck Josh R 
Published: July 2023

AbstractObligate intracellular malaria parasites dramatically remodel their erythrocyte host through effector protein export to create a niche for survival. Most exported proteins contain a pentamericPlasmodiumexportelement (PEXEL)/Host Targeting Motif that is cleaved in the parasite ER by the aspartic protease Plasmepsin V (PMV). This processing event exposes a mature N-terminus required for translocation into the host cell and is not known to occur in non-exported proteins. Here we report that the non-exported parasitophorous vacuole protein UIS2 contains abona fidePEXEL motif that is processed in theP. falciparumblood-stage. While the N-termini of exported proteins containing the PEXEL and immediately downstream ∼10 residues is sufficient to mediate translocation into the RBC, the equivalent UIS2 N-terminus does not promote export of a reporter. Curiously, the UIS2 PEXEL contains an unusual aspartic acid at the fourth position which constitutes the extreme N-terminal residue following PEXEL cleavage (P1’, RILτDE). Using a series of chimeric reporter fusions, we show that Asp at P1’ is permissive for PMV processing but abrogates export. Moreover, mutation of this single UIS2 residue to alanine enables export, reinforcing that the mature N-terminus mediates export, not PEXEL processingper se. Prompted by this observation, we further show that PEXEL sequences in the N-termini of other non-exported rhoptry proteins are also processed, suggesting that PMV may be a more general secretory maturase than previously appreciated, similar to orthologs in related apicomplexans. Our findings provide new insight into the unique N-terminal constraints that mark proteins for export.ImportanceHost erythrocyte remodeling by malaria parasite exported effector proteins is critical to parasite survival and disease pathogenesis. In the deadliest malaria parasitePlasmodium falciparum, most exported proteins undergo proteolytic maturation via recognition of the pentamericPlasmodiumexportelement (PEXEL)/Host Targeting motif by the aspartic protease Plasmepsin V (PMV) which exposes a mature N-terminus that is conducive for export into the erythrocyte host cell. While PEXEL processing is considered a unique mark of exported proteins, we demonstrate PEXEL motifs are present and processed in non-exported proteins. Importantly, we show that specific residues at the variable fourth position of the PEXEL motif inhibit export despite being permissive for processing by PMV, reinforcing that features of the mature N-terminus, and not PEXEL cleavage, identify cargo for export cargo. This opens the door to further inquiry into the nature and evolution of the PEXEL motif.
Agrobacterium-mediated Genetic Transformation and Plant Regeneration from Cotyledons in Philippine Eggplant (Solanum melongenaL.) Acc. ‘PH 11424’
Authors: Sagarbarria Mark Gabriel S. , Caraan John Albert M. , Lipio Patrick G. , Oloc-oloc Ian Bien M. , Watanabe Kazuo N. , Hautea Desiree M. 
Published: July 2023

ABSTRACTEggplant (Solanum melongenaL.) is one of the most important vegetables grown and consumed in the Philippines; hence, continuous breeding programs are vital to maintain the supply of this economically important crop. This study demonstrates the first successfulAgrobacterium-mediated genetic transformation and plant regeneration of a Philippine eggplant cultivar, ‘PH 11424’ also known as ‘Mistisa’. Cotyledons from two-week old seedlings were used as explants, which were transformed with disarmedAgrobacterium tumefaciensstrain LBA4404 harboring a binary vector for CRISPR/Cas9 expression and hygromycin phosphotransferase (HPT), an antibiotic selection marker. Growth of shoot primordia from the agro-infected explants was observed during selective culture with 7.5 ppm hygromycin, which indicated an initial transformation success. The putatively transformed shoot primordia were then transferred to an elongation medium. The elongated and hygromycin-resistant shoots were allowed to develop roots in hormone-free medium supplemented with hygromycin, and subsequently acclimatized under greenhouse conditions. The entire process took at least 5 – 6 months. Of the total 585 agro-infected explants, the regeneration efficiency of rooted shoots was 5.1%. Successful transformation was confirmed by polymerase chain reaction (PCR) amplification ofCas9. Acclimatized plants tested positive for the transgene. The transgenic eggplants successfully reached maturity, flowered, and set seed. These results demonstrate a workingAgrobacterium-mediated transformation and plant regeneration protocols using cotyledons as explants in a Philippine eggplant genotype. These biotechnology tools are critical for the successful application of genetic engineering (GE) and new breeding techniques (NBTs) in eggplant crop improvement.
Whole genome mutational analysis for tumor-informed ctDNA based MRD surveillance, treatment monitoring and biological characterization of urothelial carcinoma
Authors: Nordentoft Iver , Lindskrog Sia Viborg , Birkenkamp-Demtröder Karin , Gonzalez Santiago , Kuzman Maja , Levatic Jurica , Glavas Dunja , Ptashkin Ryan , Smadbeck James , Afterman Danielle , Lauterman Tomer , Cohen Yarin , Donenhirsh Zohar , Tavassoly Iman , Alon Ury , Frydendahl Boll Johansen Amanda , Rasmussen Mads Heilskov , Andersen Claus Lindbjerg , Polak Paz , Zviran Asaf , Oklander Boris , Agerbæk Mads , Jensen Jørgen Bjerggaard , Dyrskjøt Lars 
Published: July 2023

AbstractCirculating tumor DNA (ctDNA) can be used for sensitive detection of minimal residual disease (MRD). However, the probability of detecting ctDNA at low tumor burden is limited by the number of mutations analyzed and available plasma volume. Here we applied a tumor-informed whole genome sequencing (WGS) approach for ctDNA-based MRD detection (91% sensitivity, 92% specificity) and treatment response evaluation in 916 longitudinally collected plasma samples from 112 patients with localized muscle-invasive bladder cancer. We show that WGS-based ctDNA detection is prognostic of patient outcomes with a median lead time of 131 days over radiographic imaging. We performed genomic characterization of post-treatment plasma samples with a high ctDNA level and observed acquisition of the platinum therapy-associated mutational signatures and copy number variations not present in the primary tumors. Our results support the use of WGS for ultra-sensitive ctDNA detection and highlight the additional possibility for plasma-based tracking of tumor evolution.Statement of significanceOur study supports the clinical potential of using a WGS-based strategy for sensitive ctDNA detection in patients with MIBC. Thus, WGS-based ctDNA detection constitutes a promising option for clinical use due to low requirements for plasma input and the ease of performing WGS, eliminating the need for personalized assay design.
Inflammation and Oxidative Stress Markers in Type 2 Diabetes Patients with Advanced Carotid Atherosclerosis
Authors: Ménégaut Louise , Laubriet Aline , Crespy Valentin , Leuleu Damien , Pilot Thomas , Dongen Kevin Van , Barros Jean-Paul Pais de , Gautier Thomas , Petit Jean-Michel , Thomas Charles , Nguyen Maxime , Steinmetz Eric , Masson David 
Published: July 2023

AbstractBackgroundType 2 diabetes mellitus (T2DM) is a major global health issue and a significant risk factor for atherosclerosis. Atherosclerosis in T2DM patients has been associated with inflammation, insulin resistance, hyperglycemia, dyslipidemia, and oxidative stress. Identifying molecular features of atherosclerotic plaques in T2DM patients could provide valuable insights into the pathogenesis of the diseaseAimThe MASCADI (Arachidonic Acid Metabolism in Carotid Stenosis Plaque in Diabetic Patients) study aimed to investigate the increase of 2-arachidonoyl-lysophatidylcholine (2-AA– LPC) in carotid plaques from T2DM and control patients and to explore its association with plaque vulnerability as well as with blood and intra-plaque biomarkers altered during diabetes.ResultsIn a population of elderly, polymedicated patients with advanced stage of atherosclerosis, we found that T2DM patients had higher systemic inflammation markers, such as high-sensitivity C-reactive protein (hsCRP) and IL-1β, higher levels of oxysterols, increased triglyceride levels, and decreased HDL levels as compared to control patients. Furthermore, 2-AA-LPC was significantly enriched in plaques from diabetic patients, suggesting its potential role in diabetic atherosclerosis. Interestingly, 2-AA-LPC was not associated with systemic markers related to diabetes, such as hsCRP, triglycerides, or HDL cholesterol. However, it was significantly correlated with the levels of inflammatory markers within the plaques such as lysophospholipids and 25-hydroxycholesterol, strengthening the link between local inflammation, arachidonic acid metabolism and diabetes.ConclusionOur study is in line with a key role for inflammation in the pathogenesis of diabetic atherosclerosis and highlights the involvement of 2-AA-LPC. Further research is needed to better understand the local processes involved in the alteration of plaque composition in T2DM and to identify potential therapeutic targets.
Understanding golf tourists' memorable tourism experiences emphasizing the double mediating effects and moderating effects: the case of East Asia
Authors: Jeong Yunduk 
Published: July 2023

PurposeAlthough much of the research has examined the positive relationship between memorable tourism experiences (MTEs) and tourist satisfaction, little research has attempted to analyze the double mediating effects of cognitive and affective responses and the moderating effects of tourism motivation on the relationship. To address these gaps, this study developed a theoretical framework including MTEs, cognitive response, affective response, tourism motivation and tourist satisfaction with golf tourism using a stimulus-organism-response (SOR) theory.Design/methodology/approachThe author collected data from domestic and international tourists that attended amateur golf tournaments for non-commercial purposes as amateur athletes in Jeju and Gunsan, South Korea, in 2022. Construct validity of the measurement scale was verified by confirmatory factor analysis, factor loadings, average variance extracted and construct reliability. The reliability of the measurement scale was verified by Cronbach's analysis. The current study utilizes structural equation modeling with maximum likelihood estimation to analyze the positive relationships and double mediating effects. Jamovi statistical software was also used to conduct a moderation analysis.FindingsThe findings displayed the significant impacts of MTEs on cognitive response, affective response, and tourist satisfaction, and the positive impacts of cognitive response and affective response on tourist satisfaction. Moreover, cognitive and affective responses were found to partially mediate the aforementioned relationships and golf tourism motivation moderated the aforementioned paths.Originality/valueThe current study shows that there is a double mediating role of the cognitive and affective responses and moderating role of tourism motivation on the relationship between MTEs and tourist satisfaction and explores golf tourists who participate in an amateur golf tournament for non-commercial purposes as amateur athletes, which has largely been ignored in golf tourism research.
Entrepreneurship education reveals antecedents of intention: what really matters?
Authors: Tsaknis Panagiotis A. , Sahinidis Alexandros G. , Kavagia Chrysa A. 
Published: July 2023

Purpose
The purpose of this study is to determine if the changes that occur in entrepreneurial intention, following entrepreneurship education are related to changes in the factors of the Theory of Planned Behavior (TPB).

Design/methodology/approach
This research was conducted based on a questionnaire and used a pre-test-post-test group design. A questionnaire was filled out at the start of a university-level entrepreneurship course, and a follow-up was obtained at the conclusion of a semester-long course. Both questionnaires were fully completed by a total of 191 business students from a central Business School based in Athens with a class population of 400.

Findings
The results of this study indicated that the positive change in entrepreneurial intention after the entrepreneurship course, is caused by the positive change in perceived behavioral control.

Research limitations/implications
One limitation of this study is that it assesses entrepreneurial intention and entrepreneurial behavior which may or may not materialize. Also, the findings of this study should be evaluated across different ethnic groups, before making more conclusive generalizations. Further research on the sample’s characteristics, including gender, age, past career history, and parent’s line of work, would be adding value in similar studies. Furthermore, we indicated the antecedents of the changes in entrepreneurial intention that occurred from entrepreneurship education examining only the changes of the factors of TPB, without taking into consideration additional factors.

Practical implications
The results of this study can help employees, organizations, students, and managers to develop abilities that will aid them in navigating the uncertain future. Also, our findings can help corporate management, policymakers, governments, professors, and institutions to design entrepreneurship programs that can promote entrepreneurship in more effective ways.

Originality/value
MEMORE macro reveals a new approach between the combinations of entrepreneurship education, entrepreneurial intention, and the factors of the TPB. With these combinations we can examine if the impact of entrepreneurship education on entrepreneurial intention is caused by the impact of entrepreneurship education on the factors of the TPB.
Why common job demands are challenging for individuals with mental illness: the interaction of personal vulnerability factors and ableist norms
Authors: Hennekam Sophie , Descubes Irena 
Published: July 2023

PurposeDrawing on the job demands-resources (JD-R) model, this study aims to examine which job demands individuals with diagnosed mental illness perceive to be most challenging as they navigate the workplace, why this is the case and which resources individuals tend to mobilize to meet these demands.Design/methodology/approachThe authors draw on 257 qualitative surveys filled out by individuals with mental illness in various parts of the world.FindingsThe findings show that job demands that are common in today's workplace such as a high workload and a stressful environment are considered challenging by individuals with mental illness. Further, the authors show that this is the result of the ideal worker norm consisting of the need to be a steady performer that is confident, resilient and social with which the performer cannot comply on the one hand and the particularities of this population, such as performers' self-perceived low self-esteem, sensitivity to stress, fluctuating symptoms and difficulties with the social aspects of organizational life on the other hand.Originality/valueThe study points to the unique challenges of individuals with mental illness in the workplace and highlights the role human resource management (HRM) can play in providing support to allow this population to meet the demands of one's job more easily and thrive at work.
Rushing for security: a document analysis on the sources and effects of time pressure on organizational cybersecurity
Authors: Chowdhury Noman H. , Adam Marc T.P. , Teubner Timm 
Published: July 2023

Purpose
A growing body of research has identified time pressure as a key driver of cybersecurity (CS) risks and vulnerabilities. To strengthen CS, organizations use CS documents (e.g. best practices, guidelines and policies) intended to strengthen CS. The purpose of this paper is to provide an overview of how specifically time pressure is addressed by CS documents.

Design/methodology/approach
The authors conducted a systematic search for CS documents followed by a content analysis of the identified documents. First, the authors carried out a systematic Web search and identified 92 formal and informal CS documents (e.g. security policies, procedures, guidelines, manuals and best practices). Second, they systematically analyzed the resulting documents (n = 92), using a structured approach of data familiarization and low-/high-level coding for the identification and interpretation of themes. Based on this analysis, the authors formulated a conceptual framework that captures the sources and effects of time pressure along the themes of industry, operations and users.

Findings
The authors developed a conceptual framework that outlines the role of time pressure for the CS industry, threats and operations. This provides a shared frame of reference for researchers and practitioners to understand the antecedents and consequences of time pressure in the organizational CS context.

Research limitations/implications
While the analyzed documents acknowledge time pressure as an important factor for CS, the documents provide limited information on how to respond to these concerns. Future research could, hence, consult with CS experts and policymakers to inform the development of effective guidelines and policies on how to address time pressure in the identified areas. A dedicated analysis within each area will allow to investigate the corresponding aspects of time pressure in-depth along with a consideration for targeted guidelines and policies. Last, note that a differentiation between CS document types (e.g. formal vs informal and global vs regional) was beyond the scope of this paper and may be investigated by future work.

Originality/value
This study makes three main contributions to the CS literature. First, this study broadens the understanding of the role of time pressure in CS to consider the organizational perspective along the themes of industry, threats and operations. Second, this study provides the first comprehensive assessment of how organizations address time pressure through CS documents, and how this compares to existing research in academic literature. Third, by developing a conceptual framework, this study provides a shared frame of reference for researchers and practitioners to further develop CS documents that consider time pressure’s role in secure behavior.
Entrepreneurial ecosystem, gig economy practices and Women's entrepreneurship: the case of Lebanon
Authors: Mouazen Ali Mohamad , Hernández-Lara Ana Beatriz 
Published: July 2023

PurposeThe negative consequences of the COVID-19 pandemic and the current economic situation, especially in certain countries, have compelled organizations to shrink their hierarchies, reduce working hours, freeze hiring, and rely on gig workers to perform tasks. While these circumstances may be seen as a threat, certain vulnerable labor groups, such as women, seized the opportunity to develop entrepreneurial skills and launch their own firms. Others addressed smart platforms to engage in gig economy activities. This research investigates the aspects that drive women to be entrepreneurs, exploring the relationships between the entrepreneurial ecosystem, the gig economy, and women's entrepreneurship in a developing country.Design/methodology/approachData were collected from 300 female entrepreneurs in Lebanon through questionnaires that measured the indicators and variables of the proposed model, which was tested applying partial least square.FindingsThe results show a positive influence of the entrepreneurial ecosystem and gig economy on women's entrepreneurship, stronger in the case of entrepreneurial ecosystem elements and almost similar for opportunity and necessity entrepreneurship.Originality/valueThis research achieves empirical evidence on the relationship between the entrepreneurial ecosystem, the gig economy, and women's entrepreneurship in the case of a developing country. The originality of this paper lies in its empirical and gendered approach, considering together the effects of entrepreneurial ecosystem factors and gig economy practices on women's entrepreneurship, especially relevant in a regional context like Lebanon, where digital economy may constitute an opportunity for economically vulnerable groups.
An analysis of controlled foreign company (CFC) rules of Mauritius; a comparative study with the UK and US
Authors: Beebeejaun Ambareen 
Published: July 2023

Purpose
Numerous policies are established in Mauritius to attract foreign direct investment, but at the same time, severe concerns were raised concerning the erosion of Mauritian tax base, which is witnessed by the decrease in the percentage of tax revenue to gross domestic product in recent years. To avoid these issues, in 2019, the Mauritian legislator has domesticated the Organisation for Economic Co-operation and Development (OECD) BEPS 2013 Action 3 on controlled foreign company (CFC) in its income tax legislation. As such, the purpose of this study is to critically assess the implications of CFC rules of Mauritius to reduce tax avoidance in the light of international tax competition.

Design/methodology/approach
To achieve the research objective, this study will adopt a black letter approach by analysing the rules and regulations of various jurisdiction as well as international standards on CFCs and other tax avoidance legal provisions. A comparative analysis will be conducted between Mauritian laws on CFCs and the corresponding legislation of the UK and the USA, which are selected to assess the developed world’s position on strict CFC rules.

Findings
A hasty implementation of CFC rules leads to various complexities like interpretation issues and diminishing the competitiveness of the country to multinationals. In this respect, there is the risk of a trade-off between tax collected and foreign direct investment in the country. Consequently, the research recommends that Mauritius reforms its CFC legislation by extending the scope of tax exemptions for intra-group financing income, for the first year of CFC’s operation with the possibility of offsetting foreign taxes and for the Mauritius Revenue Authority to establish detailed guidelines on the determination of CFC income and its attribution for tax purposes in Mauritius.

Originality/value
Existing literature has to a great extent focused on the role of CFC rules as a tax avoidance measure and on the divergence or convergence between domestic CFC legislation against the OECD recommendations (Dourado, 2015; Xu, 2018; Beebeejaun et al., 2023). However, limited literature is available on the evaluation of the purpose of CFC rules enacted by a developing country being Mauritius in the context of the global competitive market, to which this research aims at filling the gap.