bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid and simultaneous identification of Lonicera Japonicae Flos and Lonicerae Flos using MLPA‐HRM and rhPCR‐HRM
Authors: Mo Jing , Wang Wenbin , Wang Yongyi , Cheng Huachun , Li Xiaofang , Wang Bo 
Published: June 2023

AbstractBackgroundLonicera Japonicae Flos (LJF) and Lonicerae Flos (LF), derived from different species of Lonicera, are two different types of traditional Chinese medicine (TCM) according to their differences in composition and effectiveness. Because of the high price, good effectiveness of the medicinal, and functional values, LJF is often adulterated by LF in the market. Therefore, two single nucleotide polymorphism‐based genotyping methods, multiplex ligation‐dependent probe amplification high‐resolution melting (MLPA‐HRM) and RNase H2‐dependant PCR (rhPCR)‐HRM, were established for the identification of LF and LJF.ResultsHighly specific MLPA probes and rhPCR‐primers were designed based on the trnL‐trnF regions. The melting temperature (Tm) of amplicons was derived from the peak of the HRM curve, and the differences in Tm values were used to identify LJF and LF. The two methods have both shown good specificity for identifying LJF and LF without cross‐reaction, and have high sensitivity with the detection limits of 0.1 ng DNA template. For mixed samples, MLPA‐HRM could detect LJF mixed with 10% LF while rhPCR‐HRM could detect 5% LF in mixed samples. The MLPA‐HRM and rhPCR‐HRM were performed on DNA extracted from 54 samples of LJF and LF randomly selected from the medicinal material market, all samples were successfully identified and further verified with DNA barcoding.ConclusionMLPA‐HRM and rhPCR‐HRM methods developed in our study not only provided fast, simple, sensitive, and practical identification of LJF and LF, but also provided technical references for the adulteration identification of other herb‐medicines, they are of great significance to the quality control and safe use of plant medicinal materials.
Changes in Nasal Tip Aesthetics Over Time Following Asian Tip Plasty
Authors: Moon Hyun , Fung Chak Yuen , Kim Jeong Heon , Jang Yong Ju 
Published: June 2023

ObjectivesThis study aimed to evaluate the sequential postoperative changes in tip aesthetics, by evaluating the aesthetic performance of the septal extension graft with or without tip grafting.MethodsA total of 62 patients who underwent rhinoplasty with tip plasty were included. Using a three‐dimensional scanner, we measured anthropometric aesthetic features of the nasal tip, including tip height, tip width, nasolabial angle, and columellar lobular angle. Preoperative and 1‐month and 12‐month postoperative anthropometric parameters were compared. The patients were grouped according to surgical techniques (i.e., septal extension only and septal extension plus tip grafting groups) and subtype of tip graft.ResultsThe 1‐month postoperative values of all four aesthetic features were significantly increased compared with the preoperative values. The tip height, tip width, and nasolabial angle at 12 months were significantly decreased compared with 1 month post‐operation values, whereas the tip height and width were still greater than the preoperative values. No difference was found between 1 and 12 month values of columellar lobular angle. There were no differences in the degree of decrease in tip height, tip width, nasolabial angle, and columellar lobular angle between the septal extension graft only and septal extension graft plus tip graft groups. There were no differences in the tip graft by subtypes, single‐ and multi‐layer tip grafts.ConclusionsIncreased tip height, tip width, and widened nasolabial angle gained immediately after septal extension grafting surgery gradually decreased over the year regardless of addition of tip graft or tip grafting methods.Level of Evidence4 Laryngoscope, 134:678–683, 2024
Convening Expert Taxonomists to Build Image Libraries for Training Automated Classifiers
Authors: Kenitz Kasia M. , Orenstein Eric C. , Anderson Clarissa R. , Barth Alexander J. , Briseño‐Avena Christian , Caron David A. , Carter Melissa L. , Eggleston Emily , Franks Peter J. S. , Fumo James T. , Jaffe Jules S. , McBeain Kelsey A. , Odell Anthony , Seech Kristi , Shipe Rebecca , Smith Jayme , Taniguchi Darcy A. A. , Venrick Elizabeth L. , Barton Andrew D. 
Published: June 2023

AbstractDigital imaging technologies are increasingly used to study life in the ocean. To deal with the large volume of image data collected over space and time, scientists employ various machine learning and deep learning algorithms to perform automated image classification. Training of classifiers requires a large number of expertly curated sets of images, a time‐consuming process that requires taxonomic knowledge and understanding of the local ecosystem. The creation of these labeled training sets is the critical bottleneck for building skillful automated classifiers. Here, we discuss how we overcame this barrier by leveraging taxonomic knowledge from a group of specialists in a workshop setting and suggest best practices for effectively organizing image annotation efforts. In our experience, this 2 day workshop proved very insightful and facilitated classification of over 4 years of plankton images obtained at Scripps Pier (La Jolla, CA), focusing on diatoms and dinoflagellates. We highlight the importance of facilitating a dialog between taxonomists and engineers to better integrate ecological goals with computational constraints, and encourage continuous involvement of taxonomic experts for successful implementation of automated classifiers.
Effect of ZnAl‐LDH on corrosion resistance of Zn–55Al alloy in NaCl solution
Authors: Yang Yuancheng , Wang Youbin , Huang Qiuyu , Zhao Jinwei , Gao Feng , Wei Yuezhou 
Published: June 2023

AbstractZnAl layered double hydroxides (Zn6Al2(OH)16CO3, LDH) are the unique phase in the corrosion products of Zn–Al coating compared with the corrosion products of pure Zn coating. The effect of LDH on the corrosion resistance of Zn–55Al coating is investigated in this work. The LDH preferentially grows on the Zn‐rich region of Zn–55Al alloy, and then gradually grows on the Al‐rich region. The prepared LDH film can effectively inhibit the formation of simonkolleite (Zn5(OH)8Cl2, ZHC), hydrozincite (Zn5(CO3)2(OH)6, HZ), and ZnO in the corrosion product film of Zn–55Al alloy, making the corrosion product film more dense and less prone to cracking, which can effectively prevent the corrosion of Zn–55Al alloy. The LDH film can effectively reduce the corrosion current density and improve the electrochemical impedance of pure Zn. In the end, the corrosion protection mechanism of LDH on the surface of Zn–55Al alloy was discussed.
Insight into corrosion inhibition mechanism of carbon steel by an algal extract as an eco‐friendly corrosion inhibitor in 0.5 M H2SO4: Experimental and molecular dynamics study
Authors: Benabbouha Tariq , Siniti Mustapha , Byadi Said , Chefira Khalil , El Attari Hassan , Barhoumi Ali , Chafi Mohammed , Chibi Fatima , Rchid Halima , Rachid Nmila 
Published: June 2023

AbstractTo explore the potential of new ecological corrosion inhibitors, we investigated the mechanism of corrosion inhibition in carbon steel using Halopitys incurvus, an algal extract available in both crude and chromatographic fractions, in a 0.5 M H2SO4 medium. Various methods were employed, including gravimetry, electrochemical impedance spectroscopy, polarization curves, scanning electron microscopy (SEM) techniques, and molecular dynamics. The crude extract exhibited a high corrosion‐inhibiting potential, with a maximum inhibitory efficiency of 85.30% at 600 mg/L. The electrochemical results indicated mixed behavior of the crude extract. Furthermore, we found that the adsorption of the crude extract onto the metal surface followed the Langmuir isotherm pattern. The SEM study confirmed our proposition that extract molecules were adsorbed onto the carbon steel surface. Using the chromatographic fractionation protocol, we were able to distinguish four main fractions, and the most effective fractions reached an inhibitory efficiency value of 96.00% at 100 mg/L. The molecular dynamics simulation confirmed the experimental results.
Vanishing exponential integrability for Riesz potentials in Morrey–Orlicz spaces
Authors: Mizuta Yoshihiro , Shimomura Tetsu 
Published: June 2023

AbstractOur aim in this note is to establish exponential integrability and vanishing exponential integrability for Riesz potentials of functions in Morrey–Orlicz spaces. Our work generalizes results by Adams and Hurri‐Syrjänen (2003) and the authors (2007) from Orlicz case. We also discuss the size of the exceptional sets by using a capacity and Hausdorff measure.
Memory Phenotypes In Early, De Novo Parkinson's Disease Patients with Mild Cognitive Impairment
Authors: Siciliano Mattia , De Micco Rosa , Russo Andrea Gerardo , Esposito Fabrizio , Sant'Elia Valeria , Ricciardi Lucia , Morgante Francesca , Russo Antonio , Goldman Jennifer G. , Chiorri Carlo , Tedeschi Gioacchino , Trojano Luigi , Tessitore Alessandro 
Published: June 2023

AbstractBackgroundMemory deficits in mild cognitive impairment related to Parkinson's disease (PD‐MCI) are quite heterogeneous, and there is no general agreement on their genesis.ObjectivesTo define memory phenotypes in de novo PD‐MCI and their associations with motor and non‐motor features and patients’ quality of life.MethodsFrom a sample of 183 early de novo patients with PD, cluster analysis was applied to neuropsychological measures of memory function of 82 patients with PD‐MCI (44.8%). The remaining patients free of cognitive impairment were considered as a comparison group (n = 101). Cognitive measures and structural magnetic resonance imaging‐based neural correlates of memory function were used to substantiate the results.ResultsA three‐cluster model produced the best solution. Cluster A (65.85%) included memory unimpaired patients; Cluster B (23.17%) included patients with mild episodic memory disorder related to a “prefrontal executive‐dependent phenotype”; Cluster C (10.97%) included patients with severe episodic memory disorder related to a “hybrid phenotype,” where hippocampal‐dependent deficits co‐occurred with prefrontal executive‐dependent memory dysfunctions. Cognitive and brain structural imaging correlates substantiated the findings. The three phenotypes did not differ in terms of motor and non‐motor features, but the attention/executive deficits progressively increased from Cluster A, through Cluster B, to Cluster C. This last cluster had worse quality of life compared to others.ConclusionsOur results demonstrated the memory heterogeneity of de novo PD‐MCI, suggesting existence of three distinct memory‐related phenotypes. Identification of such phenotypes can be fruitful in understanding the pathophysiological mechanisms underlying PD‐MCI and its subtypes and in guiding appropriate treatments. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils
Authors: Li Hongzhe , Ding Jiazhi , Zhu Longji , Xu Fei , Li Wenjing , Yao Yanpo , Cui Li 
Published: June 2023

AbstractApplication of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate‐solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial P solubilizing function in soils remains a challenge. Here, by applying both phenotype‐based single‐cell Raman with D2O labeling (Raman‐D2O) and genotype‐based high‐throughput chips targeting carbon, nitrogen and P (CNP) functional genes, the effect of RM application on microbial P solubilization in three typical farmland soils was investigated. The abundances of PSMs increased in two alkaline soils after RM application identified by single‐cell Raman D2O. RM application reduced the diversity of bacterial communities and increased the abundance of a few bacteria with reported P solubilization function. Genotypic analysis indicated that RM addition generally increased the relative abundance of CNP functional genes. A correlation analysis of the abundance of active PSMs with the abundance of soil microbes or functional genes was carried out to decipher the linkage between the phenotype and genotype of PSMs. Myxococcota and C degradation genes were found to potentially contribute to the enhanced microbial P release following RM application. This work provides important new insights into the in situ function of soil PSMs. It will lead to better harnessing of agricultural waste to mobilize soil legacy P and mitigate the P crisis.
Spatiotemporal fractionation schemes for stereotactic radiosurgery of multiple brain metastases
Authors: Torelli Nathan , Papp Dávid , Unkelbach Jan 
Published: June 2023

AbstractBackgroundStereotactic radiosurgery (SRS) is an established treatment for patients with brain metastases (BMs). However, damage to the healthy brain may limit the tumor dose for patients with multiple lesions.PurposeIn this study, we investigate the potential of spatiotemporal fractionation schemes to reduce the biological dose received by the healthy brain in SRS of multiple BMs, and also demonstrate a novel concept of spatiotemporal fractionation for polymetastatic cancer patients that faces less hurdles for clinical implementation.MethodsSpatiotemporal fractionation (STF) schemes aim at partial hypofractionation in the metastases along with more uniform fractionation in the healthy brain. This is achieved by delivering distinct dose distributions in different fractions, which are designed based on their cumulative biologically effective dose () such that each fraction contributes with high doses to complementary parts of the target volume, while similar dose baths are delivered to the normal tissue. For patients with multiple brain metastases, a novel constrained approach to spatiotemporal fractionation (cSTF) is proposed, which is more robust against setup and biological uncertainties. The approach aims at irradiating entire metastases with possibly different doses, but spatially similar dose distributions in every fraction, where the optimal dose contribution of every fraction to each metastasis is determined using a new planning objective to be added to the BED‐based treatment plan optimization problem. The benefits of spatiotemporal fractionation schemes are evaluated for three patients, each with >25 BMs.ResultsFor the same tumor BED10 and the same brain volume exposed to high doses in all plans, the mean brain BED2 can be reduced compared to uniformly fractionated plans by 9%–12% with the cSTF plans and by 13%–19% with the STF plans. In contrast to the STF plans, the cSTF plans avoid partial irradiation of the individual metastases and are less sensitive to misalignments of the fractional dose distributions when setup errors occur.ConclusionSpatiotemporal fractionation schemes represent an approach to lower the biological dose to the healthy brain in SRS‐based treatments of multiple BMs. Although cSTF cannot achieve the full BED reduction of STF, it improves on uniform fractionation and is more robust against both setup errors and biological uncertainties related to partial tumor irradiation.
Improving microstructural integrity, interstitial fluid, and blood microcirculation images from multi‐b‐value diffusion MRI using physics‐informed neural networks in cerebrovascular disease
Authors: Voorter Paulien H. M. , Backes Walter H. , Gurney‐Champion Oliver J. , Wong Sau‐May , Staals Julie , van Oostenbrugge Robert J. , van der Thiel Merel M. , Jansen Jacobus F. A. , Drenthen Gerhard S. 
Published: June 2023

PurposeTo obtain better microstructural integrity, interstitial fluid, and microvascular images from multi‐b‐value diffusion MRI data by using a physics‐informed neural network (PINN) fitting approach.MethodsTest–retest whole‐brain inversion recovery diffusion‐weighted images with multiple b‐values (IVIM: intravoxel incoherent motion) were acquired on separate days for 16 patients with cerebrovascular disease on a 3.0T MRI system. The performance of the PINN three‐component IVIM (3C‐IVIM) model fitting approach was compared with conventional fitting approaches (i.e., non‐negative least squares and two‐step least squares) in terms of (1) parameter map quality, (2) test–retest repeatability, and (3) voxel‐wise accuracy. Using the in vivo data, the parameter map quality was assessed by the parameter contrast‐to‐noise ratio (PCNR) between normal‐appearing white matter and white matter hyperintensities, and test–retest repeatability was expressed by the coefficient of variation (CV) and intraclass correlation coefficient (ICC). The voxel‐wise accuracy of the 3C‐IVIM parameters was determined by 10,000 computer simulations mimicking our in vivo data. Differences in PCNR and CV values obtained with the PINN approach versus conventional fitting approaches were assessed using paired Wilcoxon signed‐rank tests.ResultsThe PINN‐derived 3C‐IVIM parameter maps were of higher quality and more repeatable than those of conventional fitting approaches, while also achieving higher voxel‐wise accuracy.ConclusionPhysics‐informed neural networks enable robust voxel‐wise estimation of three diffusion components from the diffusion‐weighted signal. The repeatable and high‐quality biological parameter maps generated with PINNs allow for visual evaluation of pathophysiological processes in cerebrovascular disease.
Child health care nurses' experience of language screening for 2.5‐year‐old children: A qualitative study
Authors: Lindgren Fändriks Anna , Almqvist Kjerstin , Hjärthag Fredrik , Pernebo Karin 
Published: June 2023

AbstractAimTo investigate the experience of Child Health Care Nurses (CHCNs) using language screening for 2.5‐year‐old children.DesignAn exploratory qualitative design with an inductive approach.MethodData were collected through semi‐structured, interviews with Swedish CHCNs who regularly performed language screening for children. The interviews were analysed by thematic analysis.ResultsFour themes were identified: ‘The difficult visit’, ‘Explanations for language delay’, ‘Language screening across cultures’ and ‘Language screening with children exposed to adverse life events’.Patient or Public ContributionOur findings suggest that in routine care a modified procedure is used for the language screening of children aged 2.5 to secure the child's cooperation and to preserve an alliance with the parents. Consequently, the validity of the screening is called into question, particularly when it comes to children from families with origins outside the dominant culture and children exposed to adverse life events.
Risk factors for obesity in adulthood among survivors of childhood cancer
Authors: Delacourt Laurène , Allodji Rodrigue , Chappat Juliette , Haddy Nadia , El‐Fayech Chiraz , Demoor‐Goldschmidt Charlotte , Journy Neige , Bolle Stéphanie , Thomas‐Teinturier Cécile , Zidane Monia , Rubino Carole , Veres Cristina , Vu‐Bezin Giao , Berchery Delphine , Pluchart Claire , Bondiau Pierre‐Yves , Dumas Agnès , Bougas Nicolas , Grill Jacques , Dufour Christelle , Fresneau Brice , Pacquement Hélène , Diallo Ibrahima , Doz François , de Vathaire Florent 
Published: June 2023

AbstractObjectiveThe aim of this study was to identify risk factors for obesity in childhood cancer survivors (CCSs).MethodsThe study included 3199 patients of the French Childhood Cancer Survivor Study cohort, with 303 patients with obesity who had returned the self‐questionnaire. Analyses were adjusted for social deprivation index and sex.ResultsCCSs were less likely to have obesity (9.5%; 95% CI: 8.5%–10.5%) than expected from the general French population rates (12.5%; p = 0.0001). Nevertheless, brain tumor survivors were significantly more likely to develop obesity than the general French population (p = 0.0001). Compared with patients who did not receive radiotherapy to the pituitary gland, those who received a dose >5 Gy had an increased risk of obesity: relative risk 1.9 (95% CI: 1.2–3.1), 2.5 (95% CI: 1.7–3.7), and 2.6 (95% CI: 1.6–4.3), respectively, for participants with 6 to 20 Gy, 20 to 40 Gy, and ≥40 Gy of radiation. Etoposide administration significantly increased the risk of obesity (relative risk 1.7; 95% CI: 1.1–2.6). High social deprivation index was also a risk factor, just like BMI at diagnosis.ConclusionsLong‐term follow‐up of CCSs should include weight follow‐up during adulthood.
Comparing apples and oranges: Creating a comprehensive view of facility siting risks
Authors: Markham Nathan 
Published: June 2023

AbstractEnsuring that building occupants are safe from the myriad potential hazards in process facilities involves many challenges. Often, a facility siting plan is the result of several disparate studies, conducted years apart, and stitched together to try to form an overall plan. This makes it difficult to find a complete representation of potential impacts on a building, or gauge how changes to operations may impact nearby buildings. Understanding all potential impacts is critical when making investment decisions regarding mitigations of facility siting risks. Many of these challenges can be addressed by creating a single map of the site using a high‐resolution satellite image embedded into a CAD file. By utilizing layering capabilities, contours from individual scenarios can be drawn on individual layers and viewed individually or used together to create an overall map to enable siting of occupied facilities. By having each potential hazard type drawn on a single map, one can quickly evaluate the potential hazards that impact a particular area and see whether a particular potential hazard is dominant. This can help in deciding where to site buildings, whether or not a building occupancy can be increased, evaluation of efficacy of risk mitigation measures, and emergency response efforts.
HrTPS12 gene dramatically enhanced insect resistance of sea buckthorn to infection by fruit fly (Rhagoletis batava obseuriosa Kol.)
Authors: Zhao Lin , Cao Dandan , Su Zhi , Fu Xiaohong , Li Yanyan , Wei Jianrong , Liu Jianfeng 
Published: June 2023

AbstractBackgroundTerpenoids emitted from plants are important for regulating plant‐insect interaction. However, it is still unclear how terpenoids affect the host defense system. There are few reports of terpenoids' involvement in the mechanisms that regulate woody plants' insect resistance.ResultsThe (E)‐β‐ocimene of terpenes was only found in RBO‐resist leaves, and its content was higher than that of other type terpenes. Further, we also found (E)‐β‐ocimene had a significant avoidance effect on RBO and reached 87.5% of the highest avoidance rate. Meanwhile, overexpression of HrTPS12 in Arabidopsis increased the HrTPS12 expression level, (E)‐β‐ocimene content, and enhanced the defense against RBO. However, silencing HrTPS12 in sea buckthorn revealed that the expression levels of HrTPS12 and (E)‐β‐ocimene significantly decreased, causing the attraction effect on RBO.ConclusionHrTPS12 was an up‐regulator, which improves sea buckthorn resistance to RBO by regulating the synthesis of volatile (E)‐β‐ocimene. These results provide in‐depth information about the interaction between RBO and sea buckthorn and provide a theoretical basis for developing plant‐based insect repellents that can be used to manage RBO. © 2023 Society of Chemical Industry.