bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Forest malaria’ in Myanmar? Tracking transmission landscapes in a diversity of environments
Authors: Legendre Eva , Girond Florian , Herbreteau Vincent , Hoeun Sokeang , Rebaudet Stanislas , Thu Aung Myint , Rae Jade Dean , Lehot Laurent , Dieng Sokhna , Delmas Gilles , Nosten François , Gaudart Jean , Landier Jordi 
Published: September 2023

Abstract
Background
In the Greater Mekong Subregion, case–control studies and national-level analyses have shown an association between malaria transmission and forest activities. The term ‘forest malaria’ hides the diversity of ecosystems in the GMS, which likely do not share a uniform malaria risk. To reach malaria elimination goals, it is crucial to document accurately (both spatially and temporally) the influence of environmental factors on malaria to improve resource allocation and policy planning within given areas. The aim of this ecological study is to characterize the association between malaria dynamics and detailed ecological environments determined at village level over a period of several years in Kayin State, Myanmar.

Methods
We characterized malaria incidence profiles at village scale based on intra- and inter-annual variations in amplitude, seasonality, and trend over 4 years (2016–2020). Environment was described independently of village localization by overlaying a 2-km hexagonal grid over the region. Specifically, hierarchical classification on principal components, using remote sensing data of high spatial resolution, was used to assign a landscape and a climate type to each grid cell. We used conditional inference trees and random forests to study the association between the malaria incidence profile of each village, climate and landscape. Finally, we constructed eco-epidemiological zones to stratify and map malaria risk in the region by summarizing incidence and environment association information.

Results
We identified a high diversity of landscapes (n = 19) corresponding to a gradient from pristine to highly anthropogenically modified landscapes. Within this diversity of landscapes, only three were associated with malaria-affected profiles. These landscapes were composed of a mosaic of dense and sparse forest fragmented by small agricultural patches. A single climate with moderate rainfall and a temperature range suitable for mosquito presence was also associated with malaria-affected profiles. Based on these environmental associations, we identified three eco-epidemiological zones marked by later persistence of Plasmodium falciparum, high Plasmodium vivax incidence after 2018, or a seasonality pattern in the rainy season.

Conclusions
The term forest malaria covers a multitude of contexts of malaria persistence, dynamics and populations at risk. Intervention planning and surveillance could benefit from consideration of the diversity of landscapes to focus on those specifically associated with malaria transmission.

Graphical Abstract
Diversity and geographic distribution of haplotypes of Dirofilaria immitis across European endemic countries
Authors: Alsarraf Mustafa , Carretón Elena , Ciuca Lavinia , Diakou Anastasia , Dwużnik-Szarek Dorota , Fuehrer Hans-Peter , Genchi Marco , Ionică Angela Monica , Kloch Agnieszka , Kramer Laura Helen , Mihalca Andrei D. , Miterpáková Martina , Morchón Rodrigo , Papadopoulos Elias , Pękacz Mateusz , Rinaldi Laura , Alsarraf Mohammed , Topolnytska Mariia , Vismarra Alice , Zawistowska-Deniziak Anna , Bajer Anna 
Published: September 2023

Abstract
Background
Dirofilaria immitis, also known as heartworm, is one of the most important parasitic nematodes of domestic dogs, causing a potentially serious disease, cardiopulmonary dirofilariosis, which can be lethal. This species seems to be less 'expansive' than its sister species Dirofilaria repens, and it is believed that climate change facilitates the spread of this parasite to new non-endemic regions.

Methods
In total, 122 heartworm isolates were analysed from nine endemic countries in Europe (Portugal, Spain, Italy, Greece, Hungary, Romania, Slovakia, and Ukraine) and a single isolate from Bangladesh by amplification and sequencing of two mitochondrial (mt) DNA markers: cytochrome c oxidase subunit 1 (COI) and dehydrogenase subunit 1 (NADH). The main aim of the current study was to determine the genetic diversity of D. immitis and compare it with D. repens haplotype diversity and distribution. DNA was extracted from adult heartworms or microfilariae in blood. Most isolates originated from dogs (Canis lupus familiaris) while 10 isolates originated from wildlife species from Romania, including eight isolates from golden jackals (Canis aureus), one isolate from a Eurasian otter (Lutra lutra) and one isolate from a red fox (Vulpes vulpes).

Results
Median spanning network analysis was based on the combined sequence (1721 bp) obtained from two mt markers and successfully delineated nine haplotypes (Di1-Di9). Haplotype Di1 was the dominant haplotype encompassing 91 out of the 122 sequences (75%) from all nine countries and four host species. Haplotype Di2 was the second most common haplotype, formed solely by 13 isolates from Italy. The remaining sequences were assigned to Di3-Di9 haplotypes, differing by 1–4 SNPs from the dominant Di1 haplotype. There was evidence for geographical segregation of haplotypes, with three unique haplotypes associated with Italy and four others associated with certain countries (Di4 and Di7 with Slovakia; Di8 with Greece; Di6 with Hungary).

Conclusion
Diversity in D. immitis mt haplotypes was lower by half than in D. repens (9 vs. 18 haplotypes in D. immitis and D. repens, respectively), which may be associated with the slower expansion of heartworm in Central and NE Europe. NADH gene appears to be conserved in Dirofilaria sp. by showing lower genetic diversity than the analysed COI gene.

Graphical Abstract
Diaporthe cotoneastri and Ilyonectria cyclaminicola endophytes promote the growth, development, and accumulation of active components of Epimedium koreanum Nakai host plants in field experiments
Authors: Chen Jiawen , Hu Xing , Bai Yang , Liu Hanning , Zhuang Xin , Guo Junjie , Xiao Jinglei 
Published: September 2023

Abstract
Purpose
This study aims to explore the effects of two endophytes of Epimedium koreanum Nakai on host plant growth and development and medicinal material quality and to promote the growth and development of ingredients derived from Epimedium koreanum Nakai.

Methods
The effects on the biomass, photosynthesis parameters, chlorophyll fluorescence parameters, activity of key enzymes, and active components in the process of growth and development in Korea were examined.

Results
From the analysis of biomass and photosynthesis data, both the S3 (Diaporthe cotoneastri) and S10 (Ilyonectria cyclaminicola) strains promoted the growth of Epimedium koreanum Nakai. Redundancy analysis (RDA) showed that the activity of S3 and the content of epimedin A were negatively correlated and that the peroxidase (POD) activity was positively correlated with the total flavonoid content.

Conclusion
The application of the S3 (Diaporthe cotoneastri) and S10 (Ilyonectria cyclaminicola) strains can significantly increase the biomass and contents of total flavonoids, icariin, epimedin B, and epimedin C, but not epimedin A, in Epimedium koreanum Nakai host plants grown in field conditions. The S10 strain promotes faster growth of Epimedium koreanum Nakai than the S3 strain, and flavonoid 3-hydroxylase (F3H) catalyzes the synthesis of epimedin A and icariin by different pathways using the same substrate. POD plays a key role in total flavonoid synthesis in Epimedium koreanum Nakai.
The quality of care index for low back pain: a systematic analysis of the global burden of disease study 1990–2017
Authors: Ghafouri Mohammad , Ghasemi Erfan , Rostami Mohsen , Rouhifard Mahtab , Rezaei Negar , Nasserinejad Maryam , Danandeh Khashayar , Nakhostin-Ansari Amin , Ghanbari Ali , Borghei Alireza , Ahmadzadeh Amiri Ali , Teymourzadeh Azin , Taylor Jeffrey B. , Moghadam Navid , Kordi Ramin 
Published: September 2023

Abstract
Background
. Low back pain is one of the major causes of morbidity worldwide. Studies on low back pain quality of care are limited. This study aimed to evaluate the quality of care of low back pain worldwide and compare gender, age, and socioeconomic groups.

Methods
. This study used GBD data from 1990 to 2017 from the Institute for Health Metrics and Evaluation (IHME) website. Extracted data included low back pain incidence, prevalence, disability-adjusted life years (DALYs), and years lived with disability (YLDs). DALYs to prevalence ratio and prevalence to incidence ratio were calculated and used in the principal component analysis (PCA) to make a proxy of the quality-of-care index (QCI). Age groups, genders, and countries with different socioeconomic statuses regarding low back pain care quality from 1990 to 2017 were compared.

Results
The proxy of QCI showed a slight decrease from 36.44 in 1990 to 35.20 in 2017. High- and upper-middle-income countries showed a decrease in the quality of care from 43.17 to 41.57 and from 36.37 to 36.00, respectively, from 1990 to 2017. On the other hand, low and low-middle-income countries improved, from a proxy of QCI of 20.99 to 27.89 and 27.74 to 29.36, respectively.

Conclusion
. Despite improvements in the quality of care for low back pain in low and lower-middle-income countries between 1990 and 2017, there is still a large gap between these countries and higher-income countries. Continued steps must be taken to reduce healthcare barriers in these countries.
Influence of age on voice quality after transoral CO2 laser microsurgery
Authors: Lechien Jerome R. , Hans Stephane , Crevier-Buchman Lise 
Published: September 2023

Objective To study the post-operative evolution of voice quality of patients treated by transoral CO2 laser microsurgery (TLM) according to the age. Methods Patients treated by type I to VI TLM and post-operative speech therapy were prospectively recruited from our hospital. The voice quality was assessed pre-, 1-, 3- 6- and 12-month posttreatment with voice handicap index (VHI), dysphonia, roughness, breathiness, asthenia, strain (GRBAS), maximal phonation time (MPT), F0, F0 standard deviation (STD), percent jitter, percent shimmer, noise-to-harmonic ratio (NHR), vocal fold vibration assessment and speech fluency. Evolution of voice outcomes was analyzed considering age of patients (< 60 vs. ≥ 60 years). Results Seventy-five patients completed the evaluations. Thirty-four and forty-one patients were < 60 or ≥ 60 yo, respectively. Subjective and objective voice parameters (VHI, G, R, B), jitter and fluency significantly improved from 1- to 6-month post-TLM in < 60 yo individuals. The voice parameters improved 12-month post-TLM in the ≥ 60 yo group at the exception of VHI that improved 3-month post-TLM. There were positive associations between age and 12-month NHR, G and A parameters. Conclusion The post-operative evolution of voice quality parameters may vary between patients according to the age. Preoperative VHI is predictive of 12-month subjective and objective voice outcomes. Graphical Abstract
High-throughput sequencing in plant disease management: a comprehensive review of benefits, challenges, and future perspectives
Authors: Nizamani Mir Muhammad , Zhang Qian , Muhae-Ud-Din Ghulam , Wang Yong 
Published: September 2023

AbstractHigh-throughput sequencing (HTS) has instigated a paradigm shift in plant pathology, showcasing its transformative role in the management of plant diseases. As a powerful tool, HTS aids in identifying pathogens and enhances disease management strategies by detecting novel and emerging pathogens, tracking disease outbreaks, and contributing to developing disease-resistant cultivars. Despite these benefits, the implementation of HTS faces obstacles due to the complexity of data interpretation and economic factors that affect its widespread adoption. This comprehensive review summarizes the strengths, limitations, and opportunities associated with using HTS in managing plant diseases. The article also delves into the prospects of HTS, incorporating technological advancements, synergy with complementary methodologies, capacity-building initiatives, and the development of best practices and guidelines. By acknowledging and addressing these obstacles while harnessing the full capabilities of HTS, we advocate for a refined approach to managing plant diseases. This approach is critical for ensuring global food security, especially in the context of a growing global population and climate change.
Efficient degradation of various recalcitrant azo dyes in aqueous medium by immobilized Origanum vulgare peroxidase
Authors: Golbabaie Mahsa , Gharahchei Behzad , Mirazizi Fatemeh , Baharanchi Alireza Abbasi , Khosronejad Ali , Karkhanie Ali Asghar , Haghbeen Kamahldin 
Published: September 2023

AbstractHybrid xerogels, constructed from organic and inorganic silanes, have emerged as materials with versatile applications. They have shown promising potential for immobilization as their chemical structures encompass the mechanical, thermal, and structural stability of inorganic compounds in synergy with the functionality and flexibility of organic compounds. Incorporating a magnetic core and preparation at the nanoscale multiply their attraction for enzyme immobilization. To evaluate how efficiently this technology works for the immobilization of a plant peroxidase, a novel peroxidase (POXov) from a famous medicinal herb, Origanum vulgare, was immobilized on magnetic nanoparticles of a hybrid xerogel obtained from tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane. Immobilization boosted the physicochemical properties of the enzyme so that the immobilized POXov (I.POXov) could tolerate lower pHs and higher temperatures. It oxidized all types of the examined phenolic, catecholic, guaiacolic, and aniline diazo dyes, while the free POXov (F.POXov) failed to oxidize the last group. These improvements also made I.POXov capable of oxidizing the recalcitrant azo dyes carrying electron-withdrawing groups much faster than F.POXov. I.POXov was employed in 7 successive cycles of oxidation of both phenolic and guaiacolic dyes with an average efficiency of 69%. The efficacy of the immobilization method and I.POXov competency for the enzymatic remediation of polluted water resources have been quantitatively demonstrated.
“Cheerios in the lung” as first metastases from endometrial endometrioid adenocarcinoma with adequate response to immunotherapy
Authors: De Nardo Chiara , Landini Nicholas , Massaro Michele , Pernazza Angelina , Arienzo Francesca , Bicchetti Marco , Panebianco Valeria , Catalano Carlo 
Published: September 2023

Abstract
Background
Metastases from endometrial carcinoma to the lungs are uncommon and cavitating metastases are even rarer. In some cases, lung cavitating metastases may resemble diffuse “Cheerios in the lung.”

Case presentation
A 58-year-old woman, smoker, with a history of hysteroannessiectomy and pelvic lymphadenectomy for a low stage endometrial endometrioid adenocarcinoma, came under our attention for dry cough without fever that persisted for over three months, and computed tomography (CT) revealed diffuse cavitating lung metastases resembling "Cheerios in the lung".
After standard chemotherapy proved ineffective, Lenvatinib plus Pembrolizumab (immunotherapy) was administrated, leading to the disappearance of all lung nodules, leaving behind a diffuse emphysematous-like pattern.

Conclusions
This case is a rare example of metastatic endometrioid adenocarcinoma, manifested with chronic dry cough, with “Cheerios in the lung” appearence. The metastases responded well to immunotherapy, resulting in the regression of nodules into areas of diffuse emphysematous-like changes.
Splinting in carpal tunnel syndrome—should we use it during the daytime?
Authors: Kirac Unal Zeynep , Umay Ebru , Unlu Akyuz Ece 
Published: September 2023

Abstract
Background
Splinting is frequently used in the treatment of CTS (carpal tunnel syndrome) and is mostly preferred at night. On the other hand, there are some studies suggesting the use of splint throughout the day. However, there is still no consensus. The aim of this randomized controlled study was to evaluate the effects of day and night splinting in terms of pain, symptom severity, functional status, and quality of life in patients with CTS.

Results
Seventy wrists of 46 patients with CTS were evaluated. In the day-night splint group, according to without splint group and night-only splint group, a statistically significant improvement was found in terms of pain level assessed by Visual Analogue Scale (p = 0.001, p = 0.015, respectively), Boston Carpal Tunnel Questionnaire-Functional Severity Scale (p = 0.004, p = 0.020, respectively), The Nottingham Health Profile-pain (p = 0.001, p = 0.003, respectively), The Nottingham Health Profile-social isolation (p = 0.001, p = 0.001, respectively), and The Nottingham Health Profile-total score (p = 0.001, p = 0.012, respectively). In addition, it was found that even the use of splint only at night resulted in a significant reduction in pain compared to the control group (p = 0.018).

Conclusions
Continuation of splinting as much as possible during the daytime in addition to night splinting is beneficial in terms of pain, nocturnal symptoms, functionality, and quality of life.
Reconstruction and rehabilitation of the Grand Al-Nouri Mosque dated to the twelfth century: a case of study
Authors: Aboelhassan Mohamed Gamal , Tarabia Ahmed Mokhtar , Hassan Sherif Farag 
Published: September 2023

AbstractThis paper aims to present the structural design and the rehabilitation process as well as the site assessment of the reconstruction and rehabilitation of the twelfth-century Grand Al-Nouri Mosque in Al-Nouri Complex, which is a part of the project Revive the Spirit of Mosul in Iraq. UNESCO launched the project Revive the Spirit of Mosul in February 2018 in order to reconstruct the old city after the destruction and looting from the wars with ISIS (Daesh) in 2017. Al-Nouri Complex includes the Great Al-Nouri Mosque and the 45-m-high Al-Hadba Minaret, which are the iconic historic landmarks of the old city of Mosul on the western side of the Tigris River, and it was built by Nur Al-Din Zengi during the twelfth century. Al-Nouri Mosque was reconstructed in 1942 using the original 40 octagonal Moslawi marble columns, and alabaster arches, the mihrab brought from the Ummayad Mosque in Mosul dates back to 956 AD, masonry stones, and a reinforced concrete roof. In this paper, the structural performance of Al-Nouri Mosque has been investigated through site observations, experimental tests, and structural analyses. Due to the several destructive earthquakes recorded around Mosul, a 3D finite element model of the whole mosque using a commercial finite element program has been developed considering the actual material properties and the effect of wind and seismic actions. Generally, the output results of the structural analysis models showed that the obtained compressive stress values did not exceed the compressive strength values defined for the original footing stones and Moslawi marble. Also, the recommended rehabilitation methodology was proposed to enhance the structural characteristics of the dome, the original Moslawi marble columns, and the old stone footings under the marble columns.
Impact of symptomatic menopausal transition on the occurrence of depression, anxiety, and sleep disorders: A real-world multi-site study
Authors: Lee Dong Yun , Andreescu Carmen , Aizenstein Howard , Karim Helmet , Mizuno Akiko , Kolobaric Antonija , Yoon Seokyoung , Kim Yerim , Lim Jaegyun , Hwang Ein Jeong , Ouh Yung-Taek , Kim Hyung Hoi , Son Sang Joon , Park Rae Woong 
Published: September 2023

Abstract

Background
The menopause transition is a vulnerable period that can be associated with changes in mood and cognition. The present study aimed to investigate whether a symptomatic menopausal transition increases the risks of depression, anxiety, and sleep disorders.

Methods
This population-based, retrospective cohort study analysed data from five electronic health record databases in South Korea. Women aged 45–64 years with and without symptomatic menopausal transition were matched 1:1 using propensity-score matching. Subgroup analyses were conducted according to age and use of hormone replacement therapy (HRT). A primary analysis of 5-year follow-up data was conducted, and an intention-to-treat analysis was performed to identify different risk windows over 5 or 10 years. The primary outcome was first-time diagnosis of depression, anxiety, and sleep disorder. We used Cox proportional hazard models and a meta-analysis to calculate the summary hazard ratio (HR) estimates across the databases.

Results
Propensity-score matching resulted in a sample of 17,098 women. Summary HRs for depression (2.10; 95% confidence interval [CI] 1.63–2.71), anxiety (1.64; 95% CI 1.01–2.66), and sleep disorders (1.47; 95% CI 1.16–1.88) were higher in the symptomatic menopausal transition group. In the subgroup analysis, the use of HRT was associated with an increased risk of depression (2.21; 95% CI 1.07–4.55) and sleep disorders (2.51; 95% CI 1.25–5.04) when compared with non-use of HRT.

Conclusions
Our findings suggest that women with symptomatic menopausal transition exhibit an increased risk of developing depression, anxiety, and sleep disorders. Therefore, women experiencing a symptomatic menopausal transition should be monitored closely so that interventions can be applied early.
Differentiation of Idiopathic Central Precocious Puberty From Premature Thelarche Using Principal Component Analysis
Authors: Cleemann Wang Amanda , Hagen Casper P , Johannsen Trine Holm , Madsen Andre Greger , Cleemann Line Hartvig , Christiansen Peter , Main Katharina M , Juul Anders , Jensen Rikke Beck 
Published: September 2023

Abstract

Context
Nonprogressive premature thelarche (PT) is a self-limiting variant of early puberty, while idiopathic central precocious puberty (ICPP) is a disorder that causes progressive development of secondary sexual characteristics and often requires treatment. The diagnostic differentiation between these conditions is important but can be challenging since they often both initially present clinically with isolated breast development.

Objective
To describe relevant clinical variables in a large cohort of girls referred for early puberty, and to evaluate clinical and biochemical parameters to distinguish between girls with ICPP and PT.

Methods
This retrospective study included 1361 girls referred with signs of early puberty to a single, tertiary center from 2009 to 2019. We evaluated clinical presentation, medical history, growth velocity, bone age, hormonal serum concentrations, and gonadotropin-releasing hormone (GnRH) test results.

Results
Central precocious puberty was diagnosed in 11% (ICPP: n = 143, organic CPP: n = 11) girls, whereas 8% (n = 91 girls) presented with PT. Receiver operating characteristic (ROC) analysis showed several biochemical and anthropometric markers as potential parameters to differentiate between ICPP and PT; however, none were individually adequate. Principal component analysis (PCA)–derived clinical and hormone profiles could predict girls with ICPP from girls with PT with a specificity of 90% and sensitivity of 84%, outperforming any single marker.

Conclusion
Differentiation of girls with ICPP and PT can be supported by individual clinical and biochemical parameters. However, dimension reduction of clinical and hormonal profiles by PCA improved the diagnostic value, which in the future may support the diagnostic process as a supplement to the GnRH test in evaluation of pubertal disorders.