bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prenatal diagnosis of Treacher Collins syndrome: A case report and literature review
Authors: Wang Yin , Wang Yonghua , Yao Mengxia , Chen Le , Wu Suqing , Liu Yanying 
Published: May 2023

AbstractTreacher Collins syndrome (TCS) should be suspected if the triad of micrognathia, glossoptosis, and posterior cleft palate, and deformed external ears are observed during prenatal ultrasonography, excepting Pierre Robin sequence. Visualization of the fetal zygomatic bone and down‐slanting palpebral fissures are conducive to differentiation. Molecular genetics testing can establish a definite diagnosis. A 28‐year‐old pregnant Chinese woman was referred for systematic ultrasound examination at 24 weeks. Two‐dimensional and three‐dimensional ultrasound showed polyhydramnios, micrognathia, absence of nasal bone, microtia, secondary cleft palate, mandibular hypoplasia, glossoptosis, and normal limbs and vertebrae. Pierre Robin sequence was misdiagnosed with the triad of micrognathia, glossoptosis, and posterior cleft palate. Final diagnosis of TCS was confirmed by whole‐exome sequencing. Visualization of the fetal zygomatic bone and down‐slanting palpebral fissures can facilitate a differential diagnosis between Pierre Robin sequence and TCS, with the triad of micrognathia, glossoptosis, and posterior cleft palate.
Single‐cell rapid identification, in situ viability and vitality profiling, and genome‐based source‐tracking for probiotics products
Authors: Zhang Jia , Ren Lihui , Zhang Lei , Gong Yanhai , Xu Teng , Wang Xiaohang , Guo Cheng , Zhai Lei , Yu Xuejian , Li Ying , Zhu Pengfei , Chen Rongze , Jing Xiaoyan , Jing Gongchao , Zhou Shiqi , Xu Mingyue , Wang Chen , Niu Changkai , Ge Yuanyuan , Ma Bo , Shang Gaishuang , Cui Yunlong , Yao Su , Xu Jian 
Published: May 2023

AbstractRapid expansion of the probiotics industry demands fast, sensitive, comprehensive, and low‐cost strategies for quality assessment. Here, we introduce a culture‐free, one‐cell‐resolution, phenome‐genome‐combined strategy called Single‐Cell Identification, Viability and Vitality tests, and Source‐tracking (SCIVVS). For each cell directly extracted from the product, the fingerprint region of D2O‐probed single‐cell Raman spectrum (SCRS) enables species‐level identification with 93% accuracy, based on a reference SCRS database from 21 statutory probiotic species, whereas the C–D band accurately quantifies viability, metabolic vitality plus their intercellular heterogeneity. For source‐tracking, single‐cell Raman‐activated Cell Sorting and Sequencing can proceed, producing indexed, precisely one‐cell‐based genome assemblies that can reach ~99.40% genome‐wide coverage. Finally, we validated an integrated SCIVVS workflow with automated SCRS acquisition where the whole process except sequencing takes just 5 h. As it is >20‐fold faster, >10‐time cheaper, vitality‐revealing, heterogeneity‐resolving, and automation‐prone, SCIVVS is a new technological and data framework for quality assessment of live‐cell products.
Biofabrication of silver nanoparticles using Spirulina platensis: In vitro anti‐coagulant, thrombolytic and catalytic dye degradation activity
Authors: Gul Anum , Ahmed Dania , Fazil Madiha Muhammad , Aslam Tooba , Rashid Marium Abdul , Khan Hanzala , Ali Ayesha , Ali Shaukat 
Published: May 2023

AbstractGreen synthesis of nanoparticles is an emerging field due to it's biosafety and promising results. Biological systems due to their biodiversity are employed in different production processes. In this study Spirulina platensis mediated silver nanoparticles (S‐AgNPs) production was done. The Uv spectra, FTIR and SEM analysis was performed for the characterization of biosynthesized S‐AgNPs. The biocompatibility evaluation of S‐AgNPs was done through hemolysis analysis. S‐AgNPs were also evaluated for anticoagulant and thrombolytic potential. In addition to the medical applications of S‐AgNPs, silver nanoparticles have been known to show potential industrial applications among which one application is the utilization of silver nanoparticles in the degradation of toxic industrial dyes. Therefore, degradation assay of Eosin Y and Methylene Blue dyes was estimated. The SEM analysis of S‐AgNPs showed the particle size of 50–65 nm, whereas the biocompatibility analysis showed that these S‐AgNPs are biocompatible at ≤400 μM concentration. The S‐AgNPs showed good anticoagulant potential and thrombolytic potential and were able to degrade 44% of the thrombus. The S‐AgNPs significantly degraded 76% of Eosin Y within 30 min, whereas Methylene Blue was 80% degraded within 20 min (P‐value ≤ .001). To the best of our knowledge, the dye degradation of Eosin Y, thrombolytic activity and anticoagulant activity of S‐AgNPs produced from the biomass of Spirulina platensis has been reported for the first time. In the current study, we conclude that our biosynthesized S‐AgNPs showed promising medical and industrial applications and these nanoparticles can be further evaluated and upscaled for large scale applications.
Histone deacetylase MrHos3 negatively regulates the production of citrinin and pigments in Monascus ruber
Authors: Liu Qianrui , Zheng Yunfan , Liu Baixue , Tang Fufang , Shao Yanchun 
Published: May 2023

AbstractMonascus spp. can produce a variety of beneficial metabolites widely used in food and pharmaceutical industries. However, some Monascus species contain the complete gene cluster responsible for citrinin biosynthesis, which raises our concerns about the safety of their fermented products. In this study, the gene Mrhos3, encoding histone deacetylase (HDAC), was deleted to evaluate its effects on the production of mycotoxin (citrinin) and the edible pigments as well as the developmental process of Monascus ruber M7. The results showed that absence of Mrhos3 caused an enhancement of citrinin content by 105.1%, 82.4%, 111.9%, and 95.7% at the 5th, 7th, 9th, and 11th day, respectively. Furthermore, deletion of Mrhos3 increased the relative expression of citrinin biosynthetic pathway genes including pksCT, mrl1, mrl2, mrl4, mrl6, and mrl7. In addition, deletion of Mrhos3 led to an increase in total pigment content and six classic pigment components. Western blot results revealed that deletion of Mrhos3 could significantly elevate the acetylation level of H3K9, H4K12, H3K18, and total protein. This study provides an important insight into the effects of hos3 gene on the secondary metabolites production in filamentous fungi.
Impact of surgical site infection on unplanned hospital readmissions, initiation of adjuvant treatment following surgery, and disease‐free survival on patients with upper aerodigestive tract squamous cell carcinoma
Authors: Thiagarajan Shivakumar , Kantamani Teja , Sathe Pranav , Shetty Ratan , Deshmukh Anuja , Chaukar Devendra , Biswas Sanjay , Divatia Jigeeshu V. , Srivastav Sharad , Mathur Purva , Myatra Sheila Nainan 
Published: May 2023

AbstractIntroductionUnplanned hospital readmission (UHR) is an important indicator of the quality of the healthcare system in place. It has various implications for the patients and the healthcare system at large. In this article, we have attempted to understand the various factors influencing UHR and the start of adjuvant treatment following cancer surgery.Patients & MethodsIn this study adult patients above 18 years of age with upper aerodigestive tract squamous cell carcinoma who underwent surgery at our center between July 2019 to December 2019 were included in the study. Various factors influencing UHR and delay in receiving adjuvant treatment were analyzed.ResultsA total of 245 patients satisfied the inclusion criteria. Surgical site infection (SSI) was the factor that had the maximum influence on the UHR (p < 0.002, OR: 5.6, 95% CI: [1.911−16.4]) and delaying the start of adjuvant treatment (p = 0.008, OR: 3.786, 95% CI: [1.421−10.086]) on multivariate analysis. Surgery lasting for >4 h and patients who had received prior treatment tended to develop SSI postoperatively. The presence of SSI also seemed to have had a negative influence on disease‐free survival (DFS) as well.ConclusionsSSI is an important postoperative complication having major implications in terms of increased UHR and delays in starting adjuvant treatment which in turn is reflected as a poorer DFS among patients who develop SSI postoperatively.
Covalent immobilization of tyrosinase on magnetic multi‐walled carbon nanotubes for inhibitor screening
Authors: Li Yue , Jiang Xin‐Xin , Liu Hong‐Mei , Li Xiao‐wei , Luo Xiao‐Jun , Liao Xun , Zhao Yan 
Published: May 2023

The inhibition of tyrosinase is considered to be a common therapeutic strategy for some hyperpigmentation disorders. Screening of tyrosinase inhibitors is of great significance to the treatment of pigmentation diseases. In this study, tyrosinase was covalently immobilized on magnetic multi‐walled carbon nanotubes for the first time, and the immobilized tyrosinase was applied for ligand fishing of tyrosinase inhibitors from complex medicinal plants. The immobilized tyrosinase was characterized by transmission electron microscopy, atomic force microscopy, Fourier‐transform infrared spectroscopy, vibrating sample magnetometry, and thermo‐gravimetric analyzer, which indicated that tyrosinase was immobilized onto magnetic multi‐walled carbon nanotubes. The immobilized tyrosinase showed better thermal stability and reusability than the free one. The ligand was fished out from Radix Paeoniae Alba and identified as 1,2,3,4,6‐pentagalloylglucose by ultra‐performance liquid chromatography‐quadrupole time‐of‐flight high‐resolution mass spectrometry. 1,2,3,4,6‐pentagalloylglucose was found to be a tyrosinase inhibitor with similar half maximal inhibitory concentration values of 57.13 ± 0.91 μM compared to kojic acid (41.96 ± 0.78 μM). This work not only established a new method for screening tyrosinase inhibitors but also holds considerable potential for exploring the new medicinal value of medicinal plants.
Inhibition of Wnt/β‐catenin pathway overcomes therapeutic resistance to abiraterone in castration‐resistant prostate cancer
Authors: Atawia Ibrahim M. , Kushwaha Prem P. , Verma Shiv , Lin Spencer , Shankar Eswar , Abdel‐Gawad Osama , Gupta Sanjay 
Published: May 2023

AbstractAbiraterone acetate has been clinically approved for the treatment of patients with advanced‐stage prostate cancer. It reduces testosterone production by blocking the enzyme cytochrome P450 17 alpha‐hydroxylase. Despite improved survival outcomes with abiraterone, almost all patients develop therapeutic resistance and disease recurrence, progressing to a more aggressive and lethal phenotype. Bioinformatics analyses predicted activation of canonical Wnt/β‐catenin and involvement of stem cell plasticity in abiraterone‐resistant prostate cancer. Increased expression of androgen receptor (AR) and β‐catenin and their crosstalk causes activation of AR target genes and regulatory networks for which overcoming acquired resistance remains a major challenge. Here we show that co‐treatment with abiraterone and ICG001, a β‐catenin inhibitor, overcomes therapeutic resistance and significantly inhibited markers of stem cell and cellular proliferation in abiraterone‐resistant prostate cancer cells. Importantly, this combined treatment abrogated the association between AR and β‐catenin; diminished SOX9 expression from the complex more prominently in abiraterone‐resistant cells. In addition, combined treatment inhibited tumor growth in an in vivo abiraterone‐resistant xenograft model, blocked stemness, migration, invasion, and colony formation ability of cancer cells. This study opens new therapeutic opportunity for advanced‐stage castration‐resistant prostate cancer patients.
Localization degree of 4f electrons in Ce3Pb intermetallic compound: A DFT + DMFT study
Authors: Li Ru‐Song , He Yu‐Song , Dang Hong‐Tao , Cao Ze‐Lin , Zheng Xiao‐Hai 
Published: May 2023

AbstractA many‐body calculation is implemented on a strongly correlated metal Ce3Pb by density functional theory plus dynamical mean‐field theory, including 4f−4f electron correlation, 4f‐conduction electron hybridization and relativistic effects. Calculation results suggest that j = 5/2 and j = 7/2 manifolds are in partially filled and empty states, respectively. Negligible contributions of 4 f°, 4f2, and 4f3 configurations give the occupation number of 4f electrons n4f about 1.0, indicating the high 4f localization in Ce3Pb above T = 300 K, in accord with the Bader charge analysis, electron localization function and charge density difference. The detailed analysis of the spectra features yields the quasi‐particle peak, Kondo resonance peak as well as Hubbard bands close to Fermi level. Finally, the quasi‐particle band structure is estimated to compare with the potential angle‐resolved photoemission spectrum.
Long‐term safety and efficacy of sublingual asenapine for the treatment of schizophrenia: A phase III extension study with follow‐up for 52 weeks (P06125)—Secondary publication
Authors: Kinoshita Toshihiko , Takekita Yoshiteru , Hiraoka Shuichi , Tamura Fumihiro , Iwama Yasuhiro 
Published: May 2023

AbstractAfter completion of a 6‐week double‐blind trial of asenapine sublingual tablets (10 or 20 mg/day) versus placebo in Asian patients with acute exacerbation of schizophrenia, including Japanese patients, this open‐label study evaluated the safety and efficacy of a 52‐week treatment with asenapine at flexible doses. In 201 subjects, including 44 who had received placebo (P/A group) and 157 who had received asenapine (A/A group) in the feeder trial, adverse events occurred at rates of 90.9% and 85.4% and serious adverse events at rates of 11.4% and 20.4%, respectively. One patient in the P/A group died. No clinically significant abnormal measurements of body weight, body mass index, or glycated hemoglobin, fasting plasma glucose, insulin, and prolactin levels were observed. The sustained efficacy rate, as evaluated by the Positive and Negative Syndrome Scale total score and other measures, remained at approximately 50% between 6 and 12 months of treatment. These results suggest that long‐term treatment with asenapine is well tolerated and provides sustained efficacy.
A systematic review of the economic value proposition for commercially available nonsurgical weight‐loss interventions
Authors: Finkelstein Eric A. , Chodavadia Parth A. , Strombotne Kiersten 
Published: May 2023

AbstractObjectiveThe study aim was to review the economic evaluation literature of commercially available and effective nonsurgical weight‐loss interventions to investigate whether there is evidence to support claims of cost‐effectiveness (i.e., good value for money) or cost savings (i.e., a positive return on investment).MethodsRelevant databases were systematically reviewed to identify economic evaluations of commercially available weight‐loss products and services shown to result in clinically significant weight loss. Five weight‐loss medications (orlistat, liraglutide, naltrexone‐bupropion, semaglutide, and phentermine‐topiramate), two meal replacement programs (Jenny Craig, Optifast), and one behavioral intervention (Weight Watchers [WW]) that met inclusion criteria were identified. After screening, 32 relevant comparisons of cost‐effectiveness or cost savings across 20 studies were identified.ResultsTen of twenty pharmaceutical comparisons showed evidence of cost‐effectiveness based on established thresholds. Four of twelve nonpharmaceutical comparisons provided evidence of cost‐effectiveness, and five made claims of cost savings. However, methodological concerns cast doubt on the robustness of these claims.ConclusionsEvidence of cost‐effectiveness for commercially available, evidence‐based, nonsurgical weight‐loss interventions is mixed. There is no evidence for cost‐saving weight‐loss medications and only weak evidence for behavioral and weight‐loss interventions. Results provide a call to action to generate more robust evidence of the economic value proposition for these interventions.
Cochlear Implantation in Very Young Children With Single‐Sided Deafness
Authors: Patro Ankita , Holder Jourdan T. , Brown Christine L. , DeFreese Andrea , Virgin Frank , Perkins Elizabeth L. 
Published: May 2023

AbstractObjectiveCochlear implants (CIs) for single‐sided deafness (SSD) have only been approved for patients 5 years and older despite data supporting that younger children can also benefit from implantation. This study describes our institution's experience with CI for SSD in children 5 years and younger.Study DesignCase series with chart review.SettingTertiary referral center.MethodsA case series with chart review identified 19 patients up to age 5 years who underwent CI for SSD between 2014 and 2022. Baseline characteristics, perioperative complications, device usage, and speech outcomes were collected.ResultsThe median age at CI was 2.8 (range, 1.0‐5.4) years, with 15 (79%) patients being below age 5 at implantation. Etiologies of hearing loss were idiopathic (n = 8), cytomegalovirus (n = 4), enlarged vestibular aqueduct (n = 3), hypoplastic cochlear nerve (n = 3), and meningitis (n = 1). The median preoperative pure‐tone average was 90 (range, 75‐120) and 20 (range, 5‐35) dB eHL in the poor and better hearing ears, respectively. No patients had postoperative complications. Twelve patients achieved consistent device use (average, 9 h/d). Three of the seven who were not consistent users had hypoplastic cochlear nerves and/or developmental delays. The three patients with available preoperative and postoperative speech testing showed significant benefits, and five patients with available postoperative testing demonstrated speech recognition in the implanted ear when isolated from the better ear.ConclusionCI can safely be performed in younger children with SSD. Patients and families accept early implantation, as evidenced by consistent device use, and derive notable benefits in speech recognition. Candidacy can be broadened to include SSD patients under age five years, particularly individuals without hypoplastic cochlear nerves or developmental delay.
Flexible microwave absorbing composites based on polydimethylsiloxane filled with hybrid carbonaceous materials: Statistical analysis of the synergistic effect
Authors: Gabino Alessandra A. P. , Soares Bluma G. 
Published: May 2023

AbstractThe effect of the combination of carbon nanotubes (CNT) and graphene nanoplatelets (GNP) on the electromagnetic absorbing properties of polydimethylsiloxane (PDMS) composites was investigated. Samples were prepared by a low‐cost and scalable simple one‐step blending approach. The number of experiments and sample compositions were optimized by design of experiments (DOE) technique, namely, Axial Simplex Centroid. The samples were analyzed concerning rheology, electric conductivity and electromagnetic properties, such as absorptivity (reflection loss – RL). CNT was revealed to be more efficient than GNP in structuring the conductive network of filler particles. On the other hand, the hybrid filler composite with a 1:1 ratio (CNT/GNP = 0.67%:0.67%) presented the highest electric conductivity (3.53 × 10−3 S m−1) and the best overall electromagnetic results, with measured RL reaching −25 dB and effective absorption bandwidth frequency of 4.6 GHz, with sample 2 mm thick. The best calculated RL value for this sample was observed with 2.5 mm with RL value corresponding to −46.8 dB. The special cubic polynomial model was successfully adjusted to the experimental data and statistically proved the synergistic effect of the hybrid on the electromagnetic properties of PDMS composites. Finally, PDMS/CNT/GNP composites were successfully prepared by a simple, fast and inexpensive method.
Roll‐Pressed Silicon Anodes with High Reversible Volumetric Capacity Achieved by Interfacial Stabilization and Mechanical Strengthening of a Silicon/Graphene Hybrid Assembly
Authors: Kim Young Hwan , Choi Song‐Gue , Chung Kyung Yoon , Lee Geon‐Woo , Choi Yong Gil , Kim Kwang‐Bum 
Published: May 2023

AbstractApplication of Si anodes is hindered by severe capacity fading due to pulverization of Si particles during the large volume changes of Si during charge/discharge and repeated formation of the solid‐electrolyte interphase. To address these issues, considerable efforts have been devoted to the development of Si composites with conductive carbons (Si/C composites). However, Si/C composites with high C content inevitably show low volumetric capacity because of low electrode density. For practical applications, the volumetric capacity of a Si/C composite electrode is more important than gravimetric capacity, but volumetric capacity in pressed electrodes is rarely reported. Herein, a novel synthesis strategy is demonstrate for a compact Si nanoparticle/graphene microspherical assembly with interfacial stability and mechanical strength achieved by consecutively formed chemical bonds using 3‐aminopropyltriethoxysilane and sucrose. The unpressed electrode (density: 0.71 g cm−3) shows a reversible specific capacity of 1470 mAh g−1 with a high initial coulombic efficiency of 83.7% at a current density of 1 C‐rate. The corresponding pressed electrode (density: 1.32 g cm−3) exhibits high reversible volumetric capacity of 1405 mAh cm−3 and gravimetric capacity of 1520 mAh g−1 with a high initial coulombic efficiency of 80.4% and excellent cycling stability of 83% over 100 cycles at 1 C‐rate.
“Dual‐ Engineering” Strategy to Regulate NH4V4O10 as Cathodes for High‐Performance Aqueous Zinc Ion Batteries
Authors: Liu Chenfan , Zhang Yu , Cheng Huanhuan , Cai Xuanxuan , Jia Dianzeng , Lin He 
Published: May 2023

AbstractAqueous zinc ion batteries (AZIBs) have attracted attention as a promising candidate for secondary battery energy storage due to their safety and environmental benefits. However, the vanadium‐based cathode material NH4V4O10 has the problem of structural instability. In this paper, it is found by density functional theory calculation that excessive NH4+ located in the interlayer will repel the Zn2+ during the process of Zn2+ insertion. This results in the distortion of the layered structure, further affects the diffusion of Zn2+ and reduces the reaction kinetics. Therefore, part of the NH4+ is removed by heat treatment. In addition, the introduction of Al3+ into the material by hydrothermal method is able to further enhance its zinc storage properties. This dual‐engineering strategy shows excellent electrochemical performance (578.2 mAh g−1 at 0.2 A g−1). This study provides valuable insights for the development of high performance AZIBs cathode materials.
Molecular Insertion: A Master Key to Unlock Smart Photoelectric Responses of Covalent Organic Frameworks
Authors: Jiang Qiao‐Qiao , Li Ya‐Jie , Wu Qiong , Liang Ru‐Ping , Wang Xun , Zhang Rui , Wang Ying‐Ao , Liu Xin , Qiu Jian‐Ding 
Published: May 2023

AbstractCovalent organic frameworks (COFs) show potentials in prominent photoelectric responses by judicious structural design. However, from the selections of monomers and condensation reactions to the synthesis procedures, the acquisition of photoelectric COFs has to meet overmuch high conditions, limiting the breakthrough and modulation in photoelectric responses. Herein, the study reports a creative “lock‐key model” based on molecular insertion strategy. A COF with suitable cavity size, TP‐TBDA, is used as the host to load guests. Merely through the volatilization of mixed solution, TP‐TBDA and guests can be spontaneously assembled via non‐covalent interactions (NCIs) to produce molecular‐inserted COFs (MI‐COFs). The NCIs between TP‐TBDA and guests acted as a bridge to facilitate charge transfer in MI‐COFs, unlocking the photoelectric responses of TP‐TBDA. By exploiting the controllability of NCIs, the MI‐COFs can realize the smart modulation of photoelectric responses by simply changing the guest molecule, thus avoiding the arduous selection of monomers and condensation reactions required by conventional COFs. The construction of molecular‐inserted COFs circumvents complicated procedures for achieving performance improvement and modulation, providing a promising direction to construct late‐model photoelectric responsive materials.