bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel conformational filter allows unambiguous identification of specific conformational ensembles for the large NS3pro/NS2B Dengue-associated protease; Implications for the appropriate choice of protein conformations in drug discovery
Authors: Agback Tatiana , Lesovoy Dmitry , Han Xiao , Lomzov Alexander , Sun Renhua , Sandalova Tatyana , Orekhov Vladislav , Achour Adnane , Agback Peter 
Published: June 2023

Abstract
The dengue virus protease NS3pro/NS2B, a key antiviral target for drug development, has been previously reported to adopt either an “open” or a “closed” conformation in an ensemble of crystal structures with different NS2B C-terminus (NS2B) positioning. In this study, in order to unambiguous identify the specific conformational ensembles that dominates in solution we apply a novel strategy, referred to as conformational filter, based on Nuclei Magnetic Resonance (NMR) spectroscopy complemented by Molecular Dynamic (MD) calculations. This was achieved by comparison of the experimental values of the relaxation parameters in the fast dynamic time window of the back bone and methyl side chains with corresponding back calculated relaxation parameters of the different conformational ensembles obtained from free MD simulations. Our analyses of the relaxation data averaged over the ensembles populated indicate that “open” conformation of DENV-2 NS3pro/NS2B registered by X-ray but not in solution is absent or below the detection threshold. Based on this data we claim that the “open” conformation found for this part of the crystal structure of NS3pro/NS2B is probably due to crystal contacts. Importantly that conformational ensembles selected through NOE restrained MD as well as observed in crystallisation where position of co factor NS2B is located in so called “partly” open conformation was in full agreement with the conformational filter: experimental and free MD calculated relaxation parameters showed good agreement. Additionally we unambiguously showed a high probability for the conformational ensemble of the “closed” conformation.
Treatment outcomes of mechanically ventilated patients with COVID-19 and associated factors at the ICU of SPHMMC, Addis Ababa, Ethiopia
Authors: korbu Shimelis , Solomon Mariamawit , Aklog Etsegent , Zewdie Ayalew , Mamo Derejit , Keflegn Yonas , Mesfin Filagot , Deksisa Alem 
Published: June 2023

Abstract
Introduction: Coronavirus (CoV) is derived from the word ‘corona’ meaning ‘crown’ in Latin. Coronavirus causes a range of human respiratory tract infections varying from mild cold to severe respiratory distress syndrome. Nowadays, due to the limited number of ICU beds and ventilators and the increasing number of patients with COVID-19 infection requiring MV data on patient characteristics, and outcomes of critical illness from COVID-19 are needed to inform decision-making about resource allocation, critical care capacity, and treatment of patients.
Objectives: To assess outcomes and associatedfactors of patients with COVID-19 on Mechanical ventilation at SPHMMC COVID ICU, Addis Ababa, Ethiopia, 2020 G.C.
Methods: A single center cross-sectional study was used. All consecutive ICU admitted patients with RT-PCR confirmed COVID-19 and received mechanical ventilation support during the first 5 months were included. Data was collected from patient’s medical records using a structured questionnaireand the collected data was entered and analyzed using SPSS version 24.
Results:A total of 104 ICU patients with COVID-19 were included. The majority, 73.1% were male; the median age was 60 (IQR; 45-70) years, with 36.5% patients having at least one chronic medical condition. Out of 165, 104 (63%) patients needed Mechanical Ventilation. Invasive and noninvasive ventilation were used in 93(89.4%) and 11(10.6%) patients, respectively. The most common treatments used by the patients were steroid (100%), antibiotics (99%) and vaso-pressors (57.7%). Experimental antiviral therapy was used in only 1% (n=1) of patients. Overall The ICU mortality was 14.6% and 88.5% among mechanically ventilated patients. Being fatigue (AOR=7.599 ; 95% CI: 1.65–35.12), IMV ((AOR=5.28; 95% CI: 1.01–27.71), and septic shock (AOR=12.83; 95% CI: 1.46-13.11) were a significant risk factors for the ICU outcomes of the patients
Conclusion:The study showed high proportion of mortality among ICU patients who received mechanical ventilator support with low rate of discharge. Male patients and old age groups with co-morbidity were disproportionately affected by the disease condition.
Human umbilical cord/placenta-derived mesenchymal stem cell secretome attenuates intestinal fibrosis
Authors: Choi Yoon Jeong , Kim Woo Ram , Kim Duk Hwan , Kim Jee Hyun , Yoo Jun Hwan 
Published: June 2023

Abstract
Background: A significant unmet need in inflammatory bowel disease is the lack of anti-fibrotic agents targeting intestinal fibrosis. This study aimed to investigate the anti-fibrogenic properties and mechanisms of the secretome of human umbilical cord/placenta-derived mesenchymal stem cells (UC/PL-scrtm) in a murine intestinal fibrosis model and human primary intestinal myofibroblasts (HIMFs).
Methods:UC/PL-scrtm was concentrated by 15 times using a 3 kDa cut-off filter. C57BL/6 mice aged 7 weeks old were randomly assigned to one of four groups: 1) control, 2) dextran sulfate sodium (DSS), 3) DSS + secretome (late-phase treatment), and 4) DSS + secretome (early-phase treatment). Chronic DSS colitis and intestinal fibrosis was induced by three cycles of DSS administration. One DSS cycle consisted of 7 d oral DSS administration (1.75%, 2%, and 2.5% DSS), followed by 14 d of water drinking. UC/PL-scrtm was intraperitoneally administered in the late phase (from day 50, 10 times) or early phase (from day 29, 10 times) of DSS cycles. HIMFs were treated with TGF-β1 and co-treated with UC/PL-scrtm (10% of culture media) in the cellular model.
Results: In the animal study, UC/PL-scrtm reduced submucosa/muscularis propria thickness and collagen deposition, which improved intestinal fibrosis in chronic DSS colitis. The UC/PL-scrtm significantly reduced the expressions of procollagen1A1 and α-smooth muscle actin, which DSS significantly elevated. The anti-fibrogenic effect was more apparent in the UC-scrtm or early-phase treatment model. The UC/PL-scrtm reduced procollagen1A1, fibronectin, and α-smooth muscle actin expression in HIMFs in cellular model. The UC/PL-scrtm downregulated fibrogenesis by suppressing RhoA, MRTF-A, and SRF expression.
Conclusions: Human UC/PL-scrtm inhibits TGF-β1-induced fibrogenic activation in HIMFs by blocking the Rho/MRTF/SRF pathway and chronic DSS colitis-induced intestinal fibrosis. Thus, it may be regarded as a novel candidate for stem cell-based therapy of intestinal fibrosis.
Association of Metabolically Unhealthy but Normal Weight Individuals with Intracranial Atherosclerotic Stenosis
Authors: Wang Anxin , xia xue , Han Xinsheng , Xia Guangxin , Zhao Xinquan 
Published: June 2023

Abstract
Background
Obesity and metabolic syndrome (MetS) are often co-existed and associated with an increased risk of stroke. But different metabolic types may have varying risks for cardiovascular diseases. We designed this study to explore the correlation between various metabolic types and Intracranial Atherosclerotic Stenosis (ICAS).
Methods
A total of 3 620 participants from the Asymptomatic Polyvascular Abnormalities Community (APAC) study were included at baseline and follow-up. Participants were categorized into six groups according to their body mass index (BMI) and MetS status. Logistic regression was employed to evaluate the correlation between various MetS phenotypes and Intracranial Atherosclerotic Stenosis (ICAS).
Result
At 2 years of follow-up, 2 526 participants completed the measurement of ICAS, and 282 were diagnosed. Compared with the metabolic healthy normal weight group, the MUH-NW group exhibited a significant association with the prevalence of ICAS (adjusted odds ratio [OR], 1.68; 95% confidence interval [CI], 1.07–2.65); but not for metabolically unhealthy normal weight (MUH-NW) (OR, 1.34; 95% CI, 0.91–1.98) and metabolically unhealthy obesity (MUO) (OR, 1.35; 95% CI, 0.82–2.24) group.
Conclusion
The present study suggested that MUH-NW individuals had a significant association with increased risk of ICAS compared with MH-NW individuals.
Changing the Game: The Role of Women in Experiential Learning
Authors: Falk Thomas , Bartels Lara , Steimanis Ivo , Duche Vishwambhar , Vollan Björn 
Published: June 2023

Abstract

Using economic games as a learning intervention in 56 Indian communities showed that involving more women led to greater success in sustainable water management two years after the intervention. Participation of female leaders indirectly influence management improvements, as their presence increased women’s participation in the games. These findings underscore the significance of involving women in game-based learning for sustainable resource management.
A Cone-shape Mass in the Ascending Aorta: Primary Aortic Mural Thrombus
Authors: Sun Yangxue , Fang Fang , Guo Hongwei 
Published: June 2023

Abstract
Background Ascending aortic mural thrombus is a rare but potentially life-threatening disease. It can cause a variety of symptoms such as limb ischemia, visceral ischemia and stroke.
Case presentation we present a 53-year-old male patient who accidentally be found a hypodense lesion with the wall of the ascending aorta of ascending aortic space-occupying lesion on by computed tomographic (CT) scan. After excluding tumor metastasis and determining anticoagulation therapy invalid, ascending aortic replacement and coronary artery bypass grafting (CABG)were performed.
Conclusions Ascending aortic replacement is an effective treatment for patients with ascending aortic mural thrombosis.
Effects of postpartum urinary incontinence: systematic review and meta-analysis
Authors: Dai Sidi , Chen Huating , Luo Taizhen 
Published: June 2023

Abstract
Background
Postpartum urinary incontinence seriously affects the physical and mental health of women. The factors influencing postpartum urinary incontinence are still controversial in clinical research. By understanding the factors influencing postpartum urinary incontinence, we can better intervene with women in labor. Therefore, this review aimed to examine the effects of maternal postpartum urinary incontinence, so as to provide scientific references for the clinical development of prevention strategies.
Method
The research searched articles using bibliographical Databases: Embase, Pubmed, Web of Science, Cochrane Library, CBM, VIP, CNKI, and Wan Fang Data. All study designs were considered while investigating the effects of postpartum urinary incontinence. All articles prior to February 2023 were searched. Studies include laboring mothers with normal labor and delivery. A total of 28,303 women were included in the reviewed studies.
Results
We identified 5,915 potential citations, of which 32 articles assessed the effects of postpartum urinary incontinence. Meta-analysis showed that the incidence of Postpartum urinary incontinence was 26%[95%CI:(21%~30%)]. There are 12 influencing factors that affect postpartum urinary incontinence: caesarean delivery, normal delivery, age ≥ 35 years, number of deliveries ≥ 2, weight > 4 kg, perineal scoliosis, history of the urinary incontinence-related disease, maternal pre-pregnancy BMI ≥ 24 kg/m2, perineal tear, instrumental delivery, history of pelvic surgery, and prolonged second stage of labor.
Discussion
Based on the above influencing factors, we can reduce postpartum urinary incontinence by early intervention and timely prevention in patients with these risks. The following two main problems exist in this study: First, the study excluded literature with less than 2 studies on the same influencing factor and literature whose data could not be utilized or combined, which may have some selection bias. Second, this study was limited by the sample size, and some of the influencing factors were included in a small number of literatures, which did not allow for the detection of publication bias.
Prospero registration number: [CRD42023412096]
Unlocking the potential of probiotic administration in caries management: a systematic review
Authors: Lopes Pedro C. , Gomes Ana T. P. C. , Mendes Karina , Blanco Letícia , Correia Maria J. 
Published: June 2023

Abstract
Background
The use of prebiotics and/or probiotic bacteria with the potential to modulate the oral ecosystem may play an important role in the prevention and management of dental caries. To assess the evidence of the potential of pre/probiotics both in the prevention and treatment of dental caries, and therefore as adjuvant approaches in caries management, we focused on the PICO question “In individuals with caries, after probiotic administration, is there an improvement in outcomes directly related to caries risk and development?".
Methods
An extensive systematic search was conducted in electronic databases PubMed, Web of Science, Scopus and Cochrane, to identify articles with relevant data. This systematic review included trials complying the following inclusion criteria: studies performed in Humans; published in English; including the observation of patients with caries, with clear indication of the probiotic used and measurement of outcomes directly involved with the cariogenic process, including quantification of bacteria with cariogenic potential. To evaluate methodological quality of the studies, the critical assessment tool Joanna Briggs Institute was used.
Results
Eight hundred and fifty articles, potentially relevant, were identified. Following PRISMA guidelines 14 articles were included in this systematic review. Outcomes related to improvement of caries risk and development parameters were salivary counts of S. mutans and Lactobacillus, salivary pH and buffer capacity, new caries and caries activity, reduction of the cariogenic organisms content, assessed through sequencing approaches and salivary concentration of antimicrobial peptides. The probiotic most often referred with beneficial results in dental caries outcomes is Lacticaseibacillus rhamnosus being the most recommended to be included in clinical studies related to oral health. Regarding the most used administration vehicle, in studies with positive effects on the caries management, probiotic supplemented milk is considered the best administration vehicle.
Conclusions
Evidence suggests a beneficial effect of probiotic supplemented milk (Lacticaseibacillus rhamnosus) as an adjuvant for caries prevention and management. However, comparable evidence is scarce and better designed and comparable studies are needed.
Association Between ICU admission (Neutrophil + Monocyte)/Lymphocyte Ratio And 30-Day Mortality in Patients with Sepsis: A Retrospective Study from MIMIC-IV
Authors: Guo Manliang , He Wanmei , Mao Xueyan , Luo Yuling , Zeng Mian 
Published: June 2023

Abstract
Background: Sepsis is an important public health issue, and it is urgent to develop valuable indicators to predict the prognosis of sepsis. Our study aims to assess the predictive value of ICU admission (Neutrophil + Monocyte)/lymphocyte ratio (NMLR) on the 30-day mortality of sepsis patients.
Methods: A retrospective analysis was conducted in septic patients, and the data were collected from Medical Information Mart for Intensive Care IV (MIMIC-IV). Univariate and multivariate Cox regression analyses were conducted to investigate the relation between ICU admission NMLR and 30-day mortality. Restricted cubic spline (RCS) was performed to determine the optimum cut-off value of ICU admission NMLR. Survival outcomes of the two groups with different ICU admission NMLR levels were estimated using the Kaplan-Meier method and compared by the log-rank test.
Results: Finally, 7292 patients were recruited in the study, of which 1601 died within 30 days of discharge. The non-survival group had higher ICU admission NMLR values than patients in the survival group (12.24 [6.44–23.67] vs. 8.71 [4.81–16.26], P < 0.001). Univariate and multivariate Cox regression analysis demonstrated that ICU admission NMLR was an independent prognostic predictor on 30-day mortality (Univariate: P < 0.001; multivariate: P=0.011). The RCS model demonstrated the upturn and non-linear relationship between ICU admission NMLR and 30-day mortality (Nonlinearity: P=0.0124). According to the KM curve analysis,30-day survival was worse in the higher ICU admission NMLR group than that in the lower ICU admission NMLR group (Log rank test, P<0.0001).
Conclusion: The elevated ICU admission NMLR level is an independent risk factor for high 30-day mortality in patients with sepsis.
Genome-wide association study for reproduction traits in Colombian Creole Blanco Orejinegro cattle
Authors: Ríos Ana Cristina Herrera , Nasner Sindy Liliana Caivio , Londoño-Gil Marisol , Gonzalez-Herrera Luis Gabriel , Lopez-Herrera Albeiro , RINCON JUAN CARLOS 
Published: June 2023

Abstract
Reproductive traits are fundamental for beef-cattle production system profitability. However, traits such as age at first calving (AFC), calving interval (CI), and gestation length (GL) have use disadvantages in traditional breeding programs since they are measured in only one sex and may have low heritability. Another important aspect is the conservation of the genetic resources of animals adapted to the Colombian regions, which implies the preservation and rational use of the creole breeds present in the country market. Therefore, this study aimed to identify genomic regions in the creole cattle breed Blanco Orejinero (BON) that influences the reproductive traits in females and to understand their genetic architecture. The dataset used comprised 439 animals and 118,116 single nucleotide polymorphisms (SNPs) markers. The GS3 program was used to identify the SNP effects employing the BAYES Cπ methodology. The number of SNPs with effect for AFC was 25, 1,527 for CI, and 23 for GL. Some of the genes found (ECE1, EPH, EPHB2, SMARCAL1, IGFBP5, IGFBP2, FCGRT, EGFR, MUL1, PINK1, STPG1, CNGB1, TGFB1, OXTR, IL22RA1, MYOM3, OXTR, CNR2, HIVEP3, CTPS1, CXCL8, FCGRT, MREG, TMEM169, PECR, and MC1R) were previously associated with reproduction and growth traits, as well as with immune response and environmental adaptation in BON cattle. Our results evidenced a high contribution of the genetic architecture of the Colombian creole cattle breed Blanco Orejinegro that should be included in the implementation of genetic improvement and conservation programs.
Generalizable principles underly the evolution and shaping of population level SARS-CoV-2 immune markers
Authors: Nilles Eric , Roberts Kathryn , Aubin Michael de St. , Mayfield Helen , Restrepo Angela Cadavid , Dumas Devan , Garnier Salome , Etienne Marie , Duke William , Jarolim Petr , Oasan Timothy , Peña Farah , Abdalla Gabriela , Lopez Beatriz , Cruz Lucia de la , Sanchez Isaac , Murray Kristy , Baldwin Margaret , Skewes-Ramm Ronald , Paulino Cecilia Then , Lau Colleen , Kucharski Adam 
Published: June 2023

Abstract

While individual level immune responses to SARS-CoV-2 are well characterized, population immunity and the factors that drive population immune markers are largely undescribed. In this study, we examined spike antibody responses that track with infection risk amongst a household cohort in the northwest and southeast of the Dominican Republic. Our sampling period was from Aug 2021 to Nov 2022, capturing sequential waves of Delta, BA.1, BA.2, and BA.4/5 transmission. We show that population antibody levels normalized from a highly irregular to a Gaussian distribution, driven by accrued infections and antibody boosting among individuals with lower baseline immunity and waning among those with higher immunity, irrespective of interval vaccination. Using a limited number of predictor variables and out-of-sample validation methods we were able to predict S-antibody changes at the Nov 2022 timepoint with a high degree of accuracy (Pearson’s correlation coefficient 0.95 for predicted vs observed change). S-antibody level at the baseline sampling timepoint was by far the most influential predictor, demonstrated by a strong association when used as the only predictor variable (Pearson’s correlation coefficient 0.92). Findings were stable across geographically distinct study regions, suggesting drivers of immune dynamics apply equally across the Dominican Republic, and likely other countries with comparable transmission profiles. Our results suggest that given sufficient transmission, generalizable and discernable principles underly population immune dynamics. We propose that these findings can be used to delineate immune dynamics in other settings, inform transmission modeling, and guide public health priorities for SARS-CoV-2, and potentially other non-immune sterilizing emerging pathogens.
Circulation dampened heat extremes intensification over the Midwest US and amplified over Western Europe
Authors: Singh Jitendra , Sippel Sebastian , Fischer Erich 
Published: June 2023

Abstract
Heat extremes have intensified globally in recent decades, but the Midwest United States (MUS) experiences a weak cooling trend while Western Europe (WEU) shows remarkable warming trends. We show that atmospheric circulation greatly amplify warming in WEU and weaken it in the MUS. Circulation-induced trends lead to ~1°C cooling in MUS since 1979, reversing the weak thermodynamic warming effects. While in WEU, they cause ~1°C warming, contributing to one third of observed trends. The observed circulation- and thermodynamic-induced trends are outside the multi-model ensemble range for the MUS and at the edge of the range for WEU. This explains why the total observed trends in these regions are at the very low and high end of the model range. To what extent the circulation-induced trends are forced or unforced remains a key open question to constrain future projections. If circulation-induced trends are forced, WEU may experience further intensified heat extremes, while if they result from unforced internal variability, WEU warming would slow and MUS may experience rapidly intensifying heat extremes in the coming decades.
Electrochemical synthesis of poly(methylene blue) on PLA-CB conductive 3D printer filament and its electrochemical activity towards the sensitivity of uric acid
Authors: fazdoune M. El , Bahend K. , Oubella M. , Jadi S. Ben , Guerraf A. El , Bazzaoui E. A. , Garcia-Garcia F. J. , Inácio. M. José , Bazzaoui M. 
Published: June 2023

Abstract
The following study describes the electropolymerization of phenothiazine methylene blue (MB) monomer on conductive 3D printer filaments made of polylactic acid doped carbon black (PLA-CB). Poly(methylene blue) (PMB) thin layers were successfully synthesized on PLA-CB electrodes using various electrochemical techniques, including cyclic voltammetry (CV), chronopotentiometry (CP), and chronoamperometry (CA). The threshold values required to initiate the polymerization process were found to be 470 mV and 10 µA.cm˗2 for the potentiostatic and galvanostatic modes respectively. Afterward, microscopic and spectroscopic characterization by SEM, Raman, as well as, XPS confirmed the successful modification of the PLA-CB surface with a thin layer of saccharin counter ions-doped PMB. The modified electrode PMB/PLA-CB was then tested for its ability as an electrochemical sensor for uric acid. The analysis conducted by cyclic voltammetry and differential pulse voltammetry (DPV) demonstrated a faster rate for electron transfer, consequently, uric acid can be electrochemically oxidized at a high number of active sites. The modified conductive 3D printer filament tested in the present study can be considered a promising sensor, not only for uric acid but also for other analytes contained in pharmaceuticals and biological samples.