bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Artificial” gender diversity and public visibility: The case of corporate water disclosure in Indonesia
Authors: Salsabila Malisa , Adhariani Desi 
Published: December 2022

AbstractGlobal water crises need solutions from various parties including the business sector. This study examines the role of board gender diversity in supporting corporate water disclosure representing companies' efforts to use and manage water efficiently. This research also investigates whether the public visibility of a corporation, measured by the Google Search Volume (GSV) or Google Trends and which describes the monitoring function from stakeholder groups, can strengthen the association. The samples consist of 51 companies listed on the Indonesia Stock Exchange (IDX) from 2018–2021. The results showed that gender diversity as indicated by the percentage of women on the board of commissioners and directors has insignificant results, which means that the board's gender diversity has not played a role as an important determinant of corporate water accountability. In addition, the moderating role of GSV also showed insignificant results, meaning that the expected positive effect of board gender diversity on water disclosure does not intensify with high public visibility. Only two control variables, namely the Corporate Social Responsibility (CSR) committee and company size have significant results, implying that if the company has large total assets and a CSR committee in its structure, the company will tend to optimize its growth by disclosing water information as proof of its commitment towards sustainable development. The insignificant results of gender diversity and public visibility on corporate water disclosure may indicate the trivial role of diversity and the lack of stakeholders' scrutiny of the disclosure of the company's water management, which will worsen the water crises if left unattended.
Comprehensive optimization of urinary exfoliated tumor cells tests in bladder cancer with a promising microfluidic platform
Authors: Gao Fengbin , Wang Jie , Yu Yanlan , Yan Jing , Ding Guoqing 
Published: December 2022

AbstractBackgroundEnrichment of urinary exfoliated tumor cells (UETCs) is a noninvasive way of bladder cancer diagnosis, but the lack of specific capture and identification of tumor cells from the urine remains a limitation that impedes the development of liquid biopsy.MethodsThe CytoBot® 2000, a novel circulating cell isolation and enrichment platform, was used for UETCs isolation after comprehensive optimization. The commercial cell lines of bladder cancer were used in spiking assay for cell recovery test. The flow cytometry and immunofluorescent staining assays were performed for expression validation of capture target and identification markers. The performance of optimized platform was validated by 159 clinical samples and analyzed using receiver operator characteristic curve.ResultsThe chip that had a pore diameter of 15*20 μm could reduce the background residues while maintaining a higher cell recovery rate. We found that the cell capture ability of chip significantly improved after anti‐EpCam antibody encapsulation, but not with T4L6FM1. In identification system optimization, the spiking assay and validation of clinical sample showed that the performance of CK20 and DBC‐1 were better that pan‐CK in tumor cell identification, in addition, the staining quality is more legible with CK20.ConclusionThe optimized capture chip is more specific for UETCs isolation. CK20 and DBC‐1 are both sensitive biomarkers of UETCs in bladder cancer diagnosis. The performance of this optimized platform is excellent in clinical test that improves the accuracy of urine cell testing and provides a new alternative for the clinical application of BLCA liquid biopsy assessment.
Amber Light Control of Peptide Secondary Structure by a Perfluoroaromatic Azobenzene Photoswitch
Authors: Cataldi Eleonora , Raschig Martina , Gutmann Marcus , Geppert Patrick T. , Ruopp Matthias , Schock Marvin , Gerwe Hubert , Bertermann Rüdiger , Meinel Lorenz , Finze Maik , Nowak‐Król Agnieszka , Decker Michael , Lühmann Tessa 
Published: December 2022

AbstractThe incorporation of photoswitches into the molecular structure of peptides and proteins enables their dynamic photocontrol in complex biological systems. Here, a perfluorinated azobenzene derivative triggered by amber light was site‐specifically conjugated to cysteines in a helical peptide by perfluoroarylation chemistry. In response to the photoisomerization (trans→cis) of the conjugated azobenzene with amber light, the secondary structure of the peptide was modulated from a disorganized into an amphiphilic helical structure.
Divergent Functionalization of Styrenes via Radical/Polar Crossover with CO2 and Sodium Sulfinates
Authors: Benedetti Vega Kimberly , Campos Delgado José Antonio , Pugnal Lucas V. B. L. , König Burkhard , Menezes Correia José Tiago , Weber Paixão Márcio 
Published: December 2022

AbstractSulfones and carboxylic acids are prominent motifs widely present in the chemical structure of agrochemicals, pharmaceuticals and many other highly valuable compounds. Herein, we describe a conjunctive strategy for the precise installation of these functionalities onto styrenes using sodium sulfinates and CO2 as coupling partners. The protocol allowed the preparation of carboxy‐sulfonylated compounds in good yields and broad functional group tolerance. Additionally, taking advantage of the leaving group ability of the sulfone moiety, a one‐pot photocatalytic carboxy‐sulfonylation‐elimination strategy was developed for the synthesis of α‐aryl‐acrylates.
The risks of cancer in older women with BRCA pathogenic variants: How far have we come?
Authors: Metcalfe Kelly A. , Gronwald Jacek , Tung Nadine M. , McCuaig Jeanna M. , Eisen Andrea , Elser Christine , Foulkes William D. , Neuhausen Susan L. , Senter Leigha , Moller Pal , Bordeleau Louise , Fruscio Robert , Velsher Lea , Zakalik Dana , Olopade Olufunmilayo I. , Eng Charis , Pal Tuya , Cullinane Carey A. , Couch Fergus J. , Kotsopoulos Joanne , Sun Ping , Lubinski Jan , Narod Steven A. 
Published: December 2022

AbstractBackgroundThe purpose of this study was to estimate the cumulative risks of all cancers in women from 50 to 75 years of age with a BRCA1 or BRCA2 pathogenic variant.MethodsParticipants were women with BRCA1 or BRCA2 pathogenic variants from 85 centers in 16 countries. Women were eligible if they had no cancer before the age of 50 years. Participants completed a baseline questionnaire and follow‐up questionnaires every 2 years. Women were followed from age 50 until a diagnosis of cancer, death, age 75, or last follow‐up. The risk of all cancers combined from age 50 to 75 was estimated using the Kaplan–Meier method.ResultsThere were 2211 women included (1470 BRCA1 and 742 BRCA2). There were 379 cancers diagnosed in the cohort between 50 and 75 years. The actuarial risk of any cancer from age 50 to 75 was 49% for BRCA1 and 43% for BRCA2. Breast (n = 186) and ovarian (n = 45) were the most frequent cancers observed. For women who had both risk‐reducing mastectomy and bilateral salpingo‐oophorectomy before age 50, the risk of developing any cancer between age 50 and 75 was 9%.ConclusionWomen with a BRCA1 or BRCA2 pathogenic variant have a high risk of cancer between the ages of 50 and 75 years and should be counselled appropriately.
Microscopy‐Based Assessment of Fatty Acid Uptake and Lipid Accumulation in Cultured Cells
Authors: Khan Danish , Ara Tarannum , Munjal Aastha , Mishra Sneha , Sundaresan Nagalingam Ravi 
Published: December 2022

AbstractThe heart relies predominantly on the use of fatty acids to derive energy. Metabolic disorders such as obesity, insulin resistance, and diabetes pose a major risk factor for the development of heart failure. Dysregulation of lipid metabolism observed in these diseases manifests as cardiac lipotoxicity, and is associated with cardiac dysfunction. The alarming rise in the incidence of these metabolic disorders warrants the need for tools to investigate the underlying molecular mechanisms. In this article, we describe a confocal microscopy–based approach to monitor fatty acid uptake and lipid accumulation in vitro, in neonatal murine cardiomyocytes and H9c2 cells. The protocol for assessment of fatty acid uptake relies on the use of BODIPY FL C 12™ to study the kinetics of fatty acid uptake via real‐time imaging of fatty acid uptake in live cells. Importantly, it circumvents the need for radioactive labeling of fatty acids to evaluate their uptake. Similarly, the protocol for assessment of lipid accumulation relies on the use of BODIPY™ 493/503 to stain the cytosolic neutral lipid population in fixed cells. We couple these confocal microscopy–based approaches with fluorescence intensity analysis using FIJI to quantify fatty acid uptake and lipid accumulation in vitro. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Assessment of fatty acid uptakeBasic Protocol 2: Assessment of lipid accumulation
New Worm on the Block: Planarians in (Neuro)Toxicology
Authors: Ireland Danielle , Collins Eva‐Maria S. 
Published: December 2022

AbstractTraditional mammalian testing is too time‐ and cost‐intensive to keep up with the large number of environmental chemicals needing assessment. This has led to a dearth of information about the potential adverse effects of these chemicals, especially on the developing brain. Thus, there is an urgent need for rapid and cost‐effective neurotoxicity and developmental neurotoxicity testing. Because of the complexity of the brain, metabolically competent organismal models are necessary to understand the effects of chemicals on nervous system development and function on a systems level. In this overview, we showcase asexual freshwater planarians as an alternative invertebrate (“non‐animal”) organismal model for neurotoxicology research. Planarians have long been used to study the effects of chemicals on regeneration and behavior. But they have only recently moved back into the spotlight because modern molecular and computational approaches now enable quantitative high‐content and high‐throughput toxicity studies. Here, we present a short history of the use of planarians in toxicology research, highlight current techniques to measure toxicity qualitatively and quantitatively in planarians, and discuss how to further promote this non‐animal organismal system into mainstream toxicology research. The articles in this collection will help work towards this goal by providing detailed protocols that can be adopted by the community to standardize planarian toxicity testing. © 2022 Wiley Periodicals LLC.
TM2−B2 Quadruple Active Sites Supported on a Defective C3N Monolayer as Catalyst for the Electrochemical CO2 Reduction: A Theoretical Perspective
Authors: Luo Yao , Ma Zengying , Xia Xueqian , Zhong Junwen , Wu Peng , Huang Yucheng 
Published: December 2022

AbstractDeveloping high‐performance electrocatalysts for the CO2 reduction reaction (CO2RR) holds great potential to mitigate the depletion of fossil feedstocks and abate the emission of CO2. In this contribution, using density functional theory calculations, we systematically investigated the CO2RR performance catalyzed by TM2−B2 (TM=Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu) supported on a defective C3N monolayer (V‐C3N). Through the screening in terms of stability of catalyst, activity towards CO2 adsorption, and selectivity against hydrogen evolution reaction, Mn2−, Fe2−, Co2−, and Ni2−B2@V−C3N were demonstrated to be a highly promising CO2RR electrocatalyst. Due to quadruple active sites, these candidates can adsorb two or three CO2 molecules. Strikingly, different products, distributing from C1 to C2+, can be generated. The high activity originates from the synergistic effect of TM and B atoms, in which they serve as adsorption sites for the C‐ and O‐species, respectively. The high selectivity towards C2+ products at the Fe2−, and Ni2−B2 sites stems from moderate C adsorption strength but relatively weak O adsorption strength, in which a universal descriptor, that is, 0.6 ΔEC−0.4 ΔEO=−1.77 eV (ΔEC/ΔEO is the adsorption energy of C/O), was proposed. This work would offer a novel perspective for the design of high active electrocatalysts towards CO2RR and for the synthesis of C2+ compounds.
Cross‐platform immunophenotyping of human peripheral blood mononuclear cells with four high‐dimensional flow cytometry panels
Authors: Heubeck Alexander , Savage Adam , Henderson Katherine , Roll Charles , Hernandez Veronica , Torgerson Troy , Bumol Thomas , Reading Julian 
Published: December 2022

AbstractImmunophenotyping using high dimensional flow cytometry is a central component of human immune system multi‐omic studies. We present four high parameter flow cytometry panels for deep immunophenotyping of human peripheral blood mononuclear cells (PBMC). This set of four 25+ color panels include 64 cell surface markers to resolve broad immune compartment populations, as well as activation and memory of specific T, B, natural killer (NK), and myeloid lineages. Common lineage bridging markers are integrated into each panel to allow for inter‐panel quality control through major lineage frequency verification. These panels were developed using a five laser BD Symphony A5 conventional cytometer and successfully transferred to a five laser Cytek Aurora spectral cytometer capable of acquiring the panels. Nine representative PBMC samples were stained with the four phenotyping panels and acquired on both instruments to evaluate population frequency and visual staining patterns for gating between the systems. Both instruments produced comparable high quality flow cytometry data and supported our decision to acquire samples on the spectral cytometer moving forward. This modular set of panels and instrument performance metrics provide guidelines for designing flow cytometry experiments suitable for longitudinal or cross‐sectional immune profiling.
COVID‐19 pandemic experiences of older adults with dementia in community and residential care settings: Results from a US national survey
Authors: Schrack Jennifer A. , Corkum Abigail E. , Freedman Vicki A. 
Published: December 2022

AbstractIntroductionInvestigations into consequences of the US COVID‐19 pandemic for older adults with dementia have been limited.MethodsWe used the National Health and Aging Trends Study to examine self‐reported COVID‐19 infection; measures taken to limit its spread; social, behavioral, and emotional responses to the pandemic; and changes in health‐care use and provider communication. We compared adults aged ≥70 with and without dementia in community and residential care settings.ResultsIn residential care settings, infection was substantially higher and social contact less common for those with dementia. In community and residential care settings, those with dementia had 2 to 3 times the odds of reporting sleeping more often. In residential care settings, those with dementia were less likely to put off care and more likely to start telehealth.DiscussionFindings highlight the disproportionate social and behavioral consequences of the COVID‐19 pandemic for those living with dementia, particularly in residential care settings.HIGHLIGHTS
Data are from the nationally representative National Health and Aging Trends Study.
COVID‐19 infection was higher in residential care settings for those with dementia.
Social contact was less common for those with dementia in residential care.
Pandemic‐related coping behaviors differed by dementia and residential status.
In residential care, those with dementia were less likely to delay health care.
Treatment strategies and pharmacist‐led medication management for Helicobacter pylori infection
Authors: Wang Yu‐Zhen , Chen Jie , Pei Shi‐Qin , Wang Chen , Han Gang , Kan Lian‐Di , Li Liu‐Cheng 
Published: December 2022

AbstractHalf of the world's population is Helicobacter pylori carrier. Updated guidelines and consensus have been issued across regions with the main aim of reducing social transmission and increasing H. pylori eradication rate. Although alternative therapies including traditional Chinese medicine and probiotics have also been used to improve H. pylori eradication rate in clinical practice, current mainstream treatment is still dependent on triple and quadruple therapies that includes antibacterial agents (e.g., amoxicillin and furazolidone) and proton pump inhibitor. Researches also assessed the eradication rate of optimized high‐dose dual therapy in treating H. pylori infection. With the increase of antibiotic resistance rate, the treatment strategies for H. pylori infection are constantly adjusted and improved. Besides, low medication compliance is another key influencing factor for H. pylori treatment failure. Emerging studies indicate that pharmacists' intervention and new pharmaceutical care methods can enhance patient medication compliance, reduce adverse drug reactions, and improve H. pylori eradication rate. The purpose of this review is to summarize the advances in treating H. pylori infection and highlight the necessity of developing novel strategies to cope with the increasing challenges and to achieve personalized medication. Also, this review attaches great importance to pharmacists in optimizing H. pylori treatment outcomes as a routine part of therapy.
Development and field validation of RPA‐CRISPR‐Cas environmental DNA assays for the detection of brown trout (Salmo trutta) and Arctic char (Salvelinus alpinus)
Authors: Williams Molly Ann , de Eyto Elvira , Caestecker Silke , Regan Fiona , Parle‐McDermott Anne 
Published: December 2022

AbstractMolecular methods are rapidly evolving to enable nucleic acid diagnostics outside a laboratory setting. Such techniques are primarily utilizing isothermal amplification such as Recombinase Polymerase Amplification (RPA) and Loop‐Mediated Isothermal Amplification (LAMP) but are yet to be fully explored for monitoring using environmental DNA (eDNA). We previously presented an RPA‐CRISPR‐Cas approach for detection of Atlantic salmon in Ireland and Canada and in this manuscript we present a further application of this technique for monitoring of brown trout and Arctic char in the Burrishoole Catchment, Co. Mayo, Ireland. In developing these assays, we offer an alternative approach to the PCR‐based assays previously published and have evolved a streamlined approach to single‐species monitoring using RPA‐CRISPR‐Cas, reducing the fluorescence acquisition time from 2 h to 30 min. This demonstrates the applicability of using RPA‐CRISPR‐Cas assays for eDNA‐based detection beyond Atlantic salmon with the added benefit of a faster assay time without compromising detection sensitivity.
Environmental RNA applications and their associated gene targets for management and conservation
Authors: Stevens Jessica D. , Parsley Meghan B. 
Published: December 2022

AbstractEnvironmental DNA (eDNA) analysis is now a widely‐used tool to non‐invasively monitor a variety of organisms. However, eDNA applications are limited with respect to gaining information on population demography and the status of individuals within a population. Although in its infancy, environmental RNA (eRNA) analysis has the potential to reveal information about populations that may be important for conservation or management decisions. Using gene databases and current literature, we synthesized information about gene products that may be useful eRNA targets for environmental samples focusing on the model organisms Danio rerio (zebrafish), Xenopus laevis (African clawed frog), and X. tropicalis (Western clawed frog). We suggested that appropriate gene products must be expressed in tissues thought to contribute to environmental samples, must be exclusively expressed or upregulated by at least 2‐fold in portions of populations (e.g., a single age class), and must be useful to a relevant application for population monitoring. We categorized 127 total eRNA targets based on their potential applications: distinguishing and quantifying living communities, determining population age structure, determining sex ratios, and assessing population health. There are many uncertainties and current limitations that need to be addressed before eRNA can be an established method in population management including understanding the ecology of eRNA, determining methods for determining assay specificity, and applying what we know from model organisms to other species. However, we see immense potential in the utility of eRNA in gathering population‐level information and serving as an important tool for managers in the near future.
Multivariate receptor modeling with widely dispersed Lichens as bioindicators of air quality
Authors: Heiner Matthew , Grimm Taylor , Smith Hayden , Leavitt Steven D. , Christensen William F. , Carling Gregory T. , St. Clair Larry L. 
Published: December 2022

AbstractBiomonitoring studies evaluating air quality via airborne element accumulation patterns in lichens typically control variability by focusing on narrow geographic regions and short time windows. Using samples of the widespread “rock‐posy” lichen sampled across the Intermountain Region of the United States, we investigate whether accumulation patterns of generic pollution sources are detectable on broad geographic and temporal scales. We develop a novel Bayesian multivariate receptor modeling (BMRM) approach that sharpens detection and discrimination of candidate pollution sources through (i) regularization of source contributions to each sample and (ii) incorporating estimated lichen secondary chemistry as a factor. Through a simulation study, we demonstrate a distinct advantage in shrinking contributions when they are truly sparse, as would be expected with heterogeneous samples from dispersed collection sites. We contrast analyses employing both standard and sparse BMRMs, and positive matrix factorization (PMF). The sparse model better maintains source identity, as specified though informative prior distributions on elemental profiles. We advocate quantitative profile matching, which reveals that PMF primarily captures variations of the baseline profile for lichen secondary chemistry. Both PMF and BMRM results suggest that the most detectable signatures relate to aeolian dust deposition, while spatial patterns hint at sporadic anthropogenic influence.
Unlocking the resource potential of livestock waste in Bangladesh
Authors: Das Priyom , Sifatul Islam Kazi , Mijan Uddin S. M. 
Published: December 2022

AbstractCurrently, the livestock sectors of Bangladesh are blooming at an incredible rate. In addition to the higher economic gain from this sector, the burden of managing a vast amount of waste has attracted the attention of environmentalists. Producing biogas and resource recovery can be a valuable solution to manage these wastes and produce clean energy. The present analysis aims to determine the bioresource potential of livestock waste in all divisions of Bangladesh. Total biogas, bioelectricity, biofertilizer, methane, and heat energy generation potential are estimated for 2020–21. Prospective manure production from livestock waste was highest in Dhaka (50.53 Mtyr−1) and lowest in Sylhet (14.51 Mtyr−1). It is estimated that 17652.2 million m3 of biogas could have been produced from only livestock manure. The total biogas potential from livestock waste was 25520.9 million m3 in 2020–21. Prospective annual methane production was 15277.3 million m3, while 467.4 petajoules of heat energy could have been produced from this sector. The estimated bioelectricity potential was 45.4 terawatts. hours (TWh), while annual biofertilizer production could be 29.81 million tons. Using biomethane in place of diesel could avoid yearly greenhouse gas (GHG) emissions of 42,500 kilotons of CO2 equivalent and reduce 28,800 kilotons of CO2 in Bangladesh. The outcome of this study can serve as a benchmark guideline for potential investors and policymakers to ensure optimal investment to produce bioenergy from livestock waste in different regions of Bangladesh.