bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purification and biochemical characterization of the DNA binding domain of the nitrogenase transcriptional activator NifA fromGluconacetobacter diazotrophicus
Authors: Standke Heidi G. , Kim Lois , Owens Cedric P. 
Published: May 2023

AbstractNifA is a σ54activator that turns on bacterial nitrogen fixation under reducing conditions and when fixed cellular nitrogen levels are low. The redox sensing mechanism in α-proteobacterial NifA is poorly understood. In this work, we examine if a Cys pair that is part of a C(X)5C motif and located immediately upstream of NifA’s DNA binding domain is involved in redox sensing in NifA from the α-proteobacteriumGluconacetobacter diazotrophicus(Gd). We hypothesize that the Cys residues’ redox state may directly influence the DNA binding domain’s DNA binding affinity and/or alter the protein’s oligomeric sate. Two DNA binding domain constructs were generated, a longer construct (2C-DBD), consisting of the DNA binding domain with the upstream Cys pair, and a shorter construct (NC-DBD) that lacks the Cys pair. TheKdof NC-DBD for its cognate DNA sequence (nifH-UAS) is equal to 20.0 μM. TheKdof 2C-DBD for nifH-UAS when the Cys pair is oxidized is 34.5 μM. Reduction of the disulfide bond does not change the DNA binding affinity. Additional experiments indicate that the redox state of the Cys residues does not influence the secondary structure or oligomerization state of the NifA DNA binding domain. Together, these results demonstrate that the Cys pair upstream of the DNA binding domain ofGd-NifA does not regulate DNA binding or domain dimerization in a redox dependent manner. This suggests that other Cys residues in NifA, such as those located in the central AAA+domain, are responsible for redox sensing.
Differentiation and quality control of smooth muscle cells from human pluripotent stem cells via the neural crest lineage
Authors: Holt Peter J. , Davaapil Hongorzul , Shetty Deeti K. , Jacob Aishwarya G. , Sinha Sanjay 
Published: May 2023

AbstractThe Sinha laboratory has developed protocols for differentiating human pluripotent stem cells (hPSCs) into vascular smooth muscle cells along developmental lineage-specific pathways. In development, paraxial mesoderm (PM), lateral plate mesoderm (LM) and neural crest (NC) linages each give rise to smooth muscle cells significant in a location-specific manner. Induced PSCs derived from patients enduring disease provide a platform from which disease-relevant cell models can be established in the laboratory. Here we describe a robust protocol for differentiating hPSCs into vascular smooth muscle cells via a neural crest lineage and the control steps required to ensure consistently high-quality differentiated cells.
Conceptual design of a new experimental setup to simulate aircraft tyre spin-up dynamics
Authors: Mahjouri Saeed , Shabani Rasoul , Skote Martin 
Published: May 2023

Purpose
The first touchdown moment of aircraft tyres on a runway is the critical phase where maximum of the vertical and horizontal ground loads is produced. Some valuable drop tests have been performed at Langley research centre to simulate the touchdown and the spin-up dynamics. However, a long impact basin and a huge power source to accelerate and decelerate the landing gear mechanism have been used. Based on a centrifugal mechanism, the purpose of this paper is to propose the conceptual design of a new experimental setup to simulate the spin-up dynamics.

Design/methodology/approach
A schematic view of the proposed mechanism is presented, and its components are introduced. Operating condition of the system and the test procedure are discussed in detail. Finally, tyre spin-up dynamics of Boeing 747 is considered as a case study, and operating condition of the system and the related test parameters are extracted.

Findings
It is shown that the aircraft tyre spin-up dynamics can be simulated in a limited laboratory space with low energy consumption. The proposed setup enables the approach velocity, sink rate and vertical ground load to be adjusted by low power actuators. Hence, the proposed mechanism can be used to simulate the tyre spin-up dynamics of different types of aircraft.

Research limitations/implications
It is important to note that more details of the setup, including the braking and actuating mechanisms together with their control procedures, should be clarified in practice. In addition, the curved path introduced as the runway will cause errors in the results. Hence, a compromise should be made between the tyre pressure, path curvature, the induced error and the cost of the experimental setup.

Practical implications
The proposed experimental setup could be constructed in a limited space and at a relatively low cost. Low power actuators are used in the proposed system. Hence, in addition to the performance tests, fatigue tests of the landing gear mechanism will also be possible.

Originality/value
Based on a centrifugal mechanism, the conceptual design of a new experimental setup is presented for simulating the tyre spin-up dynamics of aircraft. Considering that the drag load developed during tyre spin-up following initial touchdown is an important factor governing the design of the landing gear mechanism and aircraft structure, the authors hope this paper encourages engineers to continuously make efforts to increase the transparency of the touchdown process, enabling optimisation of landing gear design.
Economic effects of sustainable agri-food production in Taiwan: Does spatial agglomeration make a difference?
Authors: Chang Yun-Cih , Luh Yir-Hueih , Hsieh Ming-Feng 
Published: May 2023

PurposeThis study investigates the economic outcomes of organic farming controlling for the four major aspects of a cropping system, including climate, genotypes, management and soil. Considering possible variations in treatment responses, this study also presents empirical evidence of heterogeneous treatment effects associated with spatial agglomeration or farm covariates.Design/methodology/approachRice farm households data taken from the 2015 Agriculture Census is merged with township-level seasonal weather data, crop suitability index and average income per capita in Taiwan. To address the selection bias problem, the authors apply the Probit-2SLS instrumental variable (IV) method in the binary treatment model under homogeneous and heterogeneous assumptions.FindingsIt is found that organic farming leads to a significantly positive effect on rice farms' economic performances in terms of cost reduction and profit growth. This positive treatment effect is more sizable with spatial agglomeration. Furthermore, the treatment effect of organic farming is found to vary with the farm characteristics such as farmland area and the number of hired workers.Practical implicationsTwo important implications for the promotion of sustainable agri-food production are inferred: (1) establishing organic agriculture specialized zones may benefit rural development; (2) providing economic incentives to small farms to expand their scale may be a more effective policy means to promote sustainable agri-food production.Originality/valueThe findings in this study complement the body of knowledge by drawing insights from the agriculture census data and providing profound evidence of the heterogeneous outcomes of organic farming due to spatial clustering and farm covariates.
Women-only training programmes as tools for professional development: analysis and outcomes of a transformative learning process
Authors: Chasserio Stephanie , Bacha Eliane 
Published: May 2023

Purpose
Based on the transformative learning theory, this paper analyses a French women-only training programme (WOTP) that aims to develop women’s soft skills in their professional contexts. This paper aims to focus on the process of personal transformation, the collective dimensions and the unexpected effects of the transformation.

Design/methodology/approach
This paper used a mixed qualitative design that mainly combines a qualitative two-step study of 47 women to assess their personal changes in terms of self-confidence, self-efficacy and assertiveness. This paper used 13 semi-structured interviews to explore the perceived changes in-depth.

Findings
The analysis shows that beyond “fixing their lack of skills” – including self-limiting behaviours, low feelings of self-efficacy and difficulty claiming one’s place – a WOTP can trigger a transformational learning experience at the individual level and can modify the surveyed women’s attitudes and behaviours at work. The results also highlight the collective dimension of transformation and, to some extent, an avenue for a societal transformation.

Practical implications
One can state that these WOTPs may positively contribute to human resources development in organisations, and that they may be considered a relevant practice in the move to promote women and gender diversity in organisations.

Originality/value
The findings reveal that, at their individual levels, these women may become agents of change by influencing and acting in their professional lives. The results stress that training women may contribute to organisational changes in terms of gender diversity. These findings contribute to the enrichment of the transformative learning theory by developing the collective and societal dimensions.
Smart home cybersecurity awareness and behavioral incentives
Authors: Douha N’guessan Yves-Roland , Renaud Karen , Taenaka Yuzo , Kadobayashi Youki 
Published: May 2023

Purpose
Smart-home security involves multilayered security challenges related to smart-home devices, networks, mobile applications, cloud servers and users. However, very few studies focus on smart-home users. This paper aims to fill this gap by investigating the potential interests of adult smart-home users in cybersecurity awareness training and nonfinancial rewards that may encourage them to adopt sound cybersecurity practices.

Design/methodology/approach
A total of 423 smart-home users between the ages of 25 and 64 completed a survey questionnaire for this study, with 224 participants from Japan and 199 from the UK.

Findings
Cultural factors considerably influence adult smart-home users’ attitudes toward cybersecurity. Specifically, cultural differences impact their willingness to participate in cybersecurity awareness training, their views on the importance of cybersecurity training for children and senior citizens and their preference for nonfinancial rewards as an incentive for good cybersecurity behavior. These results highlight the need to consider cultural differences and their potential impact when developing and implementing cybersecurity programs that target smart-home users.

Practical implications
This research has two main implications. First, it provides insights for information security professionals on the importance of designing cost-effective and time-efficient cybersecurity awareness training programs for smart-home users. Second, the findings may assist governments in establishing nonfinancial incentives to encourage greater uptake of cybersecurity practices among smart-home users.

Originality/value
The paper investigates whether adult smart-home users are willing to spend time and money to engage in cybersecurity awareness training and to encourage their children and elderly parents to participate in training, as well. In addition, the paper examines incentives, especially nonfinancial rewards, that may motivate adult smart-home users to adopt cybersecurity behaviors at home. Furthermore, the paper analyses demographic differences among smart-home users in Japan and the UK.
Substation technology selection for environment efficient power distribution system in India: an integrated AHP-TOPSIS-based approach
Authors: Trivedi Ashish , Tyagi Amit , Chichi Ouissal , Kumar Sanjeev , Trivedi Vibha 
Published: May 2023

Purpose
This study aims to provide a scientific framework for the selection of suitable substation technology in an electrical power distribution network.

Design/methodology/approach
The present paper focuses on adopting an integrated multi-criteria decision-making approach using the Delphi method, analytic hierarchy process (AHP) and technique for order preference by similarity to ideal solution (TOPSIS). The AHP is used to ascertain the criteria weights, and the TOPSIS is used for choosing the most fitting technology among choices of air-insulated substation, gas-insulated substation (GIS) and hybrid substation, to guarantee educated and supported choice.

Findings
The results reveal that the GIS is the most preferred technology by area experts, considering all the criteria and their relative preferences.

Practical implications
The current research has implications for public and private organizations responsible for the management of electricity in India, particularly the distribution system as the choice of substations is an essential component that has a strong impact on the smooth functioning and performance of the energy distribution in the country. The implementation of the chosen technology not only reduces economic losses but also contributes to the reduction of power outages, minimization of energy losses and improvement of the reliability, security, stability and quality of supply of the electrical networks.

Social implications
The study explores the impact of substation technology installation in terms of its economic and environmental challenges. It emphasizes the need for proper installation checks to avoid long-term environmental hazards. Further, it reports that the economic benefits should not come at the cost of ecological degradation.

Originality/value
The present study is the first to provide a decision support framework for the selection of substation technologies using the hybrid AHP-TOPSIS approach. It also provides a cost–benefit analysis with short-term and long-term horizons. It further pinpoints the environmental issues with the installation of substation technology.
Banking system stability in the MENA region: the impact of market power and capital requirements on banks’ risk-taking behavior
Authors: Mateev Miroslav , Nasr Tarek 
Published: May 2023

Purpose
This paper aims to investigate the impact of capital requirements and bank competition on banks' risk-taking behavior in the Middle East and North Africa (MENA) region.

Design/methodology/approach
The study combines both descriptive and analytical approaches. It considers panel data sets and adopts panel data econometric techniques like fixed effects/random effects and generalized method of moments estimator.

Findings
Regulatory capital and market competition have different effects according to the bank’s type (Islamic or conventional). The results show that the capital adequacy ratio has a significant impact on the credit risk of conventional banks (CBs) while this effect is irrelevant for Islamic banks (IBs). However, market competition plays a significant role in shaping risk-taking behavior of Islamic banking institutions. Our results indicate that banks with strong market power may pursue risky strategies in the face of increased regulatory pressure (e.g. increased minimum capital requirements). The results were robust to alternative profitability measures and endogeneity checks.

Research limitations/implications
The most important limitation is the lack of data for some banks and years, and this paper had to exclude some variables because of missing observations. The second limitation concerns the number of IBs in the sample. However, this can be overcome by including more countries from MENA and other regions where Islamic banking is a growing phenomenon.

Practical implications
Our findings call for a change in Islamic banking’s traditional business model based on the prohibition of interest. The analysis indicates that market concentration moderates the association between capital requirements and the insolvency risk of IBs but not CBs. Therefore, regulatory authorities concerned with improving financial stability in the MENA region should set up their policies differently depending on the level of banking market concentration. Finally, bank managers are requested to apply a more disciplined approach to their lending decisions and build sufficient capital conservation buffers to limit the impact of downside risk from the depletion of capital buffers during the pandemic.

Originality/value
This study addresses banks’ risk-taking behavior and stability in the MENA region, which includes banks of different types (Islamic and conventional). This paper also contributes to the literature on bank stability by identifying the most critical factors that affect bank risk and stability in the MENA region, which can be relevant in the context of the new global (COVID-19) crisis.
Happy 20-year anniversary non-GAAP earnings: a systematic review of the literature
Authors: Brosnan Mark , Duncan Keith , Hasso Tim , Hollindale Janice 
Published: May 2023

PurposeIt has been two decades since the first academic paper shone a spotlight on non-GAAP earnings. The past 20 years of research investigates concerns over the misuse of these disclosures and resulted in some significant changes to accounting and reporting standards across the globe. This paper aims to document the history of non-GAAP reporting and outline the emerging themes of the now matured practice of non-GAAP reporting.Design/methodology/approachThis systematic literature review searches two popular databases to identify the academic publications relating to non-GAAP reporting between 2002 and 2022. The paper uses bibliographic mapping to present the key statistics of the non-GAAP reporting field of research.FindingsThe non-GAAP reporting environment started out as the “wild West’ but, through regulation and public awareness, emerged as an important supplement to the traditional outputs of financial reporting. Current consensus is recent non-GAAP earnings are informative to users but there is lack of research into qualitative non-GAAP disclosures and the vast body of archival research needs triangulating with more experimental studies.Originality/valueThis paper contributes to the literature by documenting the past 20 years of non-GAAP reporting and identifying the important existing and emerging research areas concerning non-GAAP earnings disclosures.
Australian initial public offerings and US venture capital: different structures for similar early-stage funding?
Authors: Marsh Terry , Gilbey Kylie Jennifer 
Published: May 2023

PurposeAustralian Securities Exchange (ASX) initial public offerings (IPOs) are an important source of early-stage capital and have also driven a substantial increase in main-board listed companies post-millennium. By contrast, Australian venture capital (VC) funding has remained largely dormant. The opposite has occurred in the US: IPOs have fallen by half, and VC funding has surged. The authors examine the reason for this divergence between ASX IPO and US VC systems that, with their supporting ecosystems, have many features in common and function similarly. The authors explore the potential factors that could explain the US VC surge vis-à-vis Australia's VC stagnation.Design/methodology/approachThe authors’ analysis is predominantly qualitative. The authors describe the Australian listing process and its similar features and functions as for the prototypical VC. The authors also describe the developments in US VC driving its recent exceptional surge and highlight that such developments have not yet materialised on the Australian scene, where early-stage IPOs have served as a substitute.FindingsThe ASX's structure and ecosystem have been critical to its success in fostering early-stage main-board listings. While the US has succeeded in alternatively growing VC, there is an increasing concern that the latter has occurred partially because valuations are stretched, tax concessions for carried-interest capital gains are too high and corporate control benefits are becoming increasingly diluted. These developments could have important implications for Australia, where VC structures are currently being reviewed.Originality/valueTo the best of the authors’ knowledge, no prior study has attempted to bridge the broad differences in IPO and VC funding trends for early-stage companies in Australia and the USA.
Do cultural differences affect the quality of financial reporting in the EU? An analysis of Western EU countries vis a vis Eastern EU countries
Authors: Mamatzakis Emmanuel C. , Neri Lorenzo , Russo Antonella 
Published: May 2023

Purpose
This study aims to examine the impact of national culture on classification shifting in Eastern European Member States of EU Eastern European countries (EEU) vis-à-vis the Western Member States of EU (WEU). The EEU provides a unique sample to study the quality of financial reporting that the authors measure with classification shifting given that for more than five decades they were following the model of a centrally planned economy, where market-based financial reporting was absent. Yet, the EEU transitioned to a market-based economy and completed its accession to the EU.

Design/methodology/approach
This study uses a panel data set of firm year observations from 1996 and 2020 that covers the full transition of EEU. This empirical analysis is based on fixed effects panel regression analysis where the authors report a plethora of identifications.

Findings
This study finds classification shifting in the EEU countries since their transition to the market-based economy, though they have no long record of market-based financial reporting. This study also notices that cultural factors are associated with classification shifting across all Member States of the EU. This study further examines the impact of interactions between cultural characteristics and special items and reveal variability between WEU and EEU. As part of the robustness analysis, this study also tests the impact of culture on real earnings management measures for both WEU vs EEU, confirming the variability of the impact of culture on earnings management.

Research limitations/implications
Future research could explore the role of religion differences in WEU vis-à-vis EEU states, as they are also subject to cultural differences.

Practical implications
The findings are important for regulators, external monitors and investors, as they show that cultural factors affect earnings management with some variability across countries in the EU, and they should be acknowledged in policymaking.

Social implications
The findings show that cultural differences between EEU and the “old” Member States of the EU could explain classification shifting.

Originality/value
To the best of the authors’ knowledge, this is the first study that sheds light on the impact of national culture on classification shifting in EEU of EU vis-à-vis the “old” WEU of EU.