bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural valuation of rewards and punishments in posttraumatic stress disorder: a computational approach
Authors: Jia Ruonan , Ruderman Lital , Pietrzak Robert H. , Gordon Charles , Ehrlich Daniel , Horvath Mark , Mirchandani Serena , DeFontes Clara , Southwick Steven , Krystal John H. , Harpaz-Rotem Ilan , Levy Ifat 
Published: March 2023

AbstractPosttraumatic stress disorder (PTSD) is associated with changes in fear learning and decision-making, suggesting involvement of the brain’s valuation system. Here we investigate the neural mechanisms of subjective valuation of rewards and punishments in combat veterans. In a functional MRI study, male combat veterans with a wide range of posttrauma symptoms (N = 48, Clinician Administered PTSD Scale, CAPS-IV) made a series of choices between sure and uncertain monetary gains and losses. Activity in the ventromedial prefrontal cortex (vmPFC) during valuation of uncertain options was associated with PTSD symptoms, an effect which was consistent for gains and losses, and specifically driven by numbing symptoms. In an exploratory analysis, computational modeling of choice behavior was used to estimate the subjective value of each option. The neural encoding of subjective value varied as a function of symptoms. Most notably, veterans with PTSD exhibited enhanced representations of the saliency of gains and losses in the neural valuation system, especially in ventral striatum. These results suggest a link between the valuation system and the development and maintenance of PTSD, and demonstrate the significance of studying reward and punishment processing within subject.
Butyrate potentiates Enterococcus faecalis lipoteichoic acid-induced inflammasome activation via histone deacetylase inhibition
Authors: Park Ok-Jin , Ha Ye-Eun , Sim Ju-Ri , Lee Dongwook , Lee Eun-Hye , Kim Sun-Young , Yun Cheol-Heui , Han Seung Hyun 
Published: March 2023

AbstractEnterococcus faecalis, a Gram-positive opportunistic pathogen having lipoteichoic acid (LTA) as a major virulence factor, is closely associated with refractory apical periodontitis. Short-chain fatty acids (SCFAs) are found in the apical lesion and may affect inflammatory responses induced by E. faecalis. In the current study, we investigated inflammasome activation by E. faecalis LTA (Ef.LTA) and SCFAs in THP-1 cells. Among SCFAs, butyrate in combination with Ef.LTA markedly enhanced caspase-1 activation and IL-1β secretion whereas these were not induced by Ef.LTA or butyrate alone. Notably, LTAs from Streptococcus gordonii, Staphylococcus aureus, and Bacillus subtilis also showed these effects. Activation of TLR2/GPCR, K+ efflux, and NF-κB were necessary for the IL-1β secretion induced by Ef.LTA/butyrate. The inflammasome complex comprising NLRP3, ASC, and caspase-1 was activated by Ef.LTA/butyrate. In addition, caspase-4 inhibitor diminished IL-1β cleavage and release, indicating that non-canonical activation of the inflammasome is also involved. Ef.LTA/butyrate induced Gasdermin D cleavage, but not the release of the pyroptosis marker, lactate dehydrogenase. This indicated that Ef.LTA/butyrate induces IL-1β production without cell death. Trichostatin A, a histone deacetylase (HDAC) inhibitor, enhanced Ef.LTA/butyrate-induced IL-1β production, indicating that HDAC is engaged in the inflammasome activation. Furthermore, Ef.LTA and butyrate synergistically induced the pulp necrosis that accompanies IL-1β expression in the rat apical periodontitis model. Taken all these results together, Ef.LTA in the presence of butyrate is suggested to facilitate both canonical- and non-canonical inflammasome activation in macrophages via HDAC inhibition. This potentially contributes to dental inflammatory diseases such as apical periodontitis, particularly associated with Gram-positive bacterial infection.
A regulatory variant at 19p13.3 is associated with primary biliary cholangitis risk and ARID3A expression
Authors: Li You , Li Zhiqiang , Chen Ruiling , Lian Min , Wang Hanxiao , Wei Yiran , You Zhengrui , Zhang Jun , Li Bo , Li Yikang , Huang Bingyuan , Chen Yong , Liu Qiaoyan , Lyu Zhuwan , Liang Xueying , Miao Qi , Xiao Xiao , Wang Qixia , Fang Jingyuan , Shi YongYong , Liu Xiangdong , Seldin Michael F. , Gershwin M. Eric , Tang Ruqi , Ma Xiong 
Published: March 2023

AbstractGenome-wide association studies have identified 19p13.3 locus associated with primary biliary cholangitis (PBC). Here we aim to identify causative variant(s) and initiate efforts to define the mechanism by which the 19p13.3 locus variant(s) contributes to the pathogenesis of PBC. A genome-wide meta-analysis of 1931 PBC subjects and 7852 controls in two Han Chinese cohorts confirms the strong association between 19p13.3 locus and PBC. By integrating functional annotations, luciferase reporter assay and allele-specific chromatin immunoprecipitation, we prioritize rs2238574, an AT-Rich Interaction Domain 3A (ARID3A) intronic variant, as a potential causal variant at 19p13.3 locus. The risk allele of rs2238574 shows higher binding affinity of transcription factors, leading to an increased enhancer activity in myeloid cells. Genome-editing demonstrates the regulatory effect of rs2238574 on ARID3A expression through allele-specific enhancer activity. Furthermore, knock-down of ARID3A inhibits myeloid differentiation and activation pathway, and overexpression of the gene has the opposite effect. Finally, we find ARID3A expression and rs2238574 genotypes linked to disease severity in PBC. Our work provides several lines of evidence that a non-coding variant regulates ARID3A expression, presenting a mechanistic basis for association of 19p13.3 locus with the susceptibility to PBC.
Thawing permafrost poses environmental threat to thousands of sites with legacy industrial contamination
Authors: Langer Moritz , von Deimling Thomas Schneider , Westermann Sebastian , Rolph Rebecca , Rutte Ralph , Antonova Sofia , Rachold Volker , Schultz Michael , Oehme Alexander , Grosse Guido 
Published: March 2023

AbstractIndustrial contaminants accumulated in Arctic permafrost regions have been largely neglected in existing climate impact analyses. Here we identify about 4500 industrial sites where potentially hazardous substances are actively handled or stored in the permafrost-dominated regions of the Arctic. Furthermore, we estimate that between 13,000 and 20,000 contaminated sites are related to these industrial sites. Ongoing climate warming will increase the risk of contamination and mobilization of toxic substances since about 1100 industrial sites and 3500 to 5200 contaminated sites located in regions of stable permafrost will start to thaw before the end of this century. This poses a serious environmental threat, which is exacerbated by climate change in the near future. To avoid future environmental hazards, reliable long-term planning strategies for industrial and contaminated sites are needed that take into account the impacts of cimate change.
Hyperloop-like diffusion of long-chain molecules under confinement
Authors: Yuan Jiamin , Gao Mingbin , Liu Zhiqiang , Tang Xiaomin , Tian Yu , Ma Gang , Ye Mao , Zheng Anmin 
Published: March 2023

AbstractThe ultrafast transport of adsorbates in confined spaces is a goal pursued by scientists. However, diffusion will be generally slower in nano-channels, as confined spaces inhibit motion. Here we show that the movement of long-chain molecules increase with a decrease in pore size, indicating that confined spaces promote transport. Inspired by a hyperloop running on a railway, we established a superfast pathway for molecules in zeolites with nano-channels. Rapid diffusion is achieved when the long-chain molecules keep moving linearly, as well as when they run along the center of the channel, while this phenomenon do not exist for short-chain molecules. This hyperloop-like diffusion is unique for long-chain molecules in a confined space and is further verified by diffusion experiments. These results offer special insights into molecule diffusion under confinement, providing a reference for the selection of efficient catalysts with rapid transport in the industrial field.
The influence of the BDNF Val66Met genotype on emotional recognition memory in post-traumatic stress disorder
Authors: Nicholson Emma Louise , Garry Michael I. , Ney Luke J. , Hsu Chia-Ming K. , Zuj Daniel V. , Felmingham Kim L. 
Published: March 2023

AbstractDysregulated consolidation of emotional memories is a core feature of posttraumatic stress disorder (PTSD). Brain Derived Neurotrophic Factor (BDNF) influences synaptic plasticity and emotional memory consolidation. The BDNF Val66Met polymorphism has been associated with PTSD risk and memory deficits respectively, although findings have been inconsistent, potentially due to a failure to control for important confounds such as sex, ethnicity, and the timing/extent of previous trauma experiences. Furthermore, very little research has examined the impact of BDNF genotypes on emotional memory in PTSD populations. This study investigated the interaction effects of Val66Met and PTSD symptomatology in an emotional recognition memory task in 234 participants divided into healthy control (n = 85), trauma exposed (TE: n = 105) and PTSD (n = 44) groups. Key findings revealed impaired negative recognition memory in PTSD compared to control and TE groups and in participants with the Val/Met compared to the Val/Val genotype. There was a group × genotype interaction showing no Met effect in the TE group despite significant effects in PTSD and controls. Results suggest that people previously exposed to trauma who do not develop PTSD may be protected from the BDNF Met effect, however more research is needed to replicate findings and to explore the epigenetic and neural processes involved.
Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins
Authors: Tomihari Ayaka , Kiyota Mako , Matsuura Akira , Itakura Eisuke 
Published: March 2023

AbstractProteostasis regulates protein folding and degradation; its maintenance is essential for resistance to stress and aging. The loss of proteostasis is associated with many age-related diseases. Within the cell, molecular chaperones facilitate the refolding of misfolded proteins into their bioactive forms, thus preventing undesirable interactions and aggregation. Although the mechanisms of intracellular protein degradation pathways for intracellular misfolded proteins have been extensively studied, the protein degradation pathway for extracellular proteins remain poorly understood. In this study, we identified several misfolded proteins that are substrates for alpha 2-macroglobulin (α2M), an extracellular chaperone. We also established a lysosomal internalization assay for α2M, which revealed that α2M mediates the lysosomal degradation of extracellular misfolded proteins. Comparative analyses of α2M and clusterin, another extracellular chaperone, indicated that α2M preferentially targets aggregation-prone proteins. Thus, we present the degradation pathway of α2M, which interacts with aggregation-prone proteins for lysosomal degradation via selective internalization.
UV–Visible–NIR camouflage textiles with natural plant based natural dyes on natural fibre against woodland combat background for defence protection
Authors: Hossain Md. Anowar 
Published: March 2023

AbstractWoodland combat background (CB) is a common source of natural plant based natural dyes (NPND). Swietenia Macrophylla, Mangifera Indica, Terminalia Arjuna, Corchorus Capsularis, Camellia Sinensis, Azadirachta Indica, Acacia Acuminata, Areca Catechu and Cinnamomum Tamala were dried-grinded-powdered-extracted-polyaziridine encapsulated-dyed-coated-printed with leafy design on cotton fabric and tested against woodland CB under the reflection engineering of ultraviolet (UV)–visible (Vis)–near infrared (NIR) spectrums and photographic versus chromatic techniques of Vis imaging. The reflection properties of NPND treated and untreated cotton fabric were experimented by UV–Vis–NIR spectrophotometer from 220 to 1400 nm. Six segments of field trialling for NPND treated woodland camouflage textiles were also investigated for concealment, detection, recognition and identification of target signature against forest plants/herbs species; common tree of woodland CB such as Shorea Robusta Gaertn, Bamboo Vulgaris, Musa Acuminata; and a wooden bridge made by Eucalyptus Citriodora & Bamboo Vulgaris. The imaging properties such as CIE L*, a*, b* and RGB (red, green, blue) of NPND treated cotton-garments were captured by digital camera from 400 to 700 nm against tree stem/bark, dry leaves, green leaves and dry wood of woodland CB. Therefore, a colorful matching for concealment, detection, recognition and identification of target signature against woodland CB was verified by Vis camera imaging and UV–Vis–NIR reflection mechanism. UV-protection property of Swietenia Macrophylla treated cotton fabric was also investigated by diffuse reflection for defence clothing. Simultaneous ‘camouflage textiles in UV–Vis–NIR’ and ‘UV-protective’ property of Swietenia Macrophylla treated fabric have been investigated for NPND materials-based textiles coloration (dyeing-coating-printing) which is a new concept for camouflage formulation of NPND dyed-NPND mordanted-NPND coated-NPND printed textiles in terms of ecofriendly source of woodland camouflage materials. Therefore, technical properties of NPND materials and methodologies of camouflage textile assessment have been advanced in addition to coloration philosophy of natural dyed-coated-printed textiles.
Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis
Authors: Alhamid Galyah , Tombuloglu Huseyin , Al-Suhaimi Ebtesam 
Published: March 2023

AbstractThe reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. However, a high false-positive rate due to misamplification is one of the major limitations. To overcome misamplifications, we developed colorimetric and fluorometric RT-LAMP assays using five LAMP primers, instead of six. The gold-standard RT-PCR technique verified the assays' performance. Compared to other primer sets with six primers (N, S, and RdRp), the E-ID1 primer set, including five primers, performed superbly on both colorimetric and fluorometric assays. The sensitivity of colorimetric and fluorometric assays was 89.5% and 92.2%, respectively, with a limit of detection of 20 copies/µL. The colorimetric RT-LAMP had a specificity of 97.2% and an accuracy of 94.5%, while the fluorometric RT-LAMP obtained 99% and 96.7%, respectively. No misamplification was evident even after 120 min, which is crucial for the success of this technique. These findings are important to support the use of RT-LAMP in the healthcare systems in fighting COVID-19.
GABAergic synapses onto SST and PV interneurons in the CA1 hippocampal region show cell-specific and integrin-dependent plasticity
Authors: Brzdąk Patrycja , Lebida Katarzyna , Wyroślak Marcin , Mozrzymas Jerzy W. 
Published: March 2023

AbstractIt is known that GABAergic transmission onto pyramidal neurons shows different forms of plasticity. However, GABAergic cells innervate also other inhibitory interneurons and plasticity phenomena at these projections remain largely unknown. Several mechanisms underlying plastic changes, both at inhibitory and excitatory synapses, show dependence on integrins, key proteins mediating interaction between intra- and extracellular environment. We thus used hippocampal slices to address the impact of integrins on long-term plasticity of GABAergic synapses on specific inhibitory interneurons (containing parvalbumin, PV + or somatostatin, SST +) known to innervate distinct parts of principal cells. Administration of RGD sequence-containing peptide induced inhibitory long-term potentiation (iLTP) at fast-spiking (FS) PV + as well as on SST + interneurons. Interestingly, treatment with a more specific peptide GA(C)RRETAWA(C)GA (RRETAWA), affecting α5β1 integrins, resulted in iLTP in SST + and iLTD in FS PV + interneurons. Brief exposure to NMDA is known to induce iLTP at GABAergic synapses on pyramidal cells. Intriguingly, application of this protocol for considered interneurons evoked iLTP in SST + and iLTD in PV + interneurons. Moreover, we showed that in SST + cells, NMDA-evoked iLTP depends on the incorporation of GABAA receptors containing α5 subunit to the synapses, and this iLTP is occluded by RRETAWA peptide, indicating a key role of α5β1 integrins. Altogether, our results revealed that plasticity of inhibitory synapses at GABAergic cells shows interneuron-specificity and show differences in the underlying integrin-dependent mechanisms. This is the first evidence that neuronal disinhibition may be a highly plastic process depending on interneuron type and integrins’ activity.
Individual differences in selective attention and engagement shape students’ learning from visual cues and instructor presence during online lessons
Authors: King Jill , Marcus Taylor , Markant Julie 
Published: March 2023

AbstractAlthough some researchers recommend minimizing extraneous visual information in multimedia lessons, others have demonstrated that features such as visual cues and instructor videos can enhance learning. However, variability in selective attention skills may influence students’ ability to benefit from these additional features. This study investigated links between college students’ selective attention skills and their learning from video lessons that varied in the use of visual cues and the instructor video. Learning outcomes depended on both the visual features available and students’ effort and selective attention skills. Among students who reported increased effort during the lessons, those with more efficient selective attention benefited most when a single additional feature (i.e., either visual cues or the instructor video) was used. All students, regardless of attention skills, benefited when both visual cues and the instructor were combined. These findings suggest that learning during multimedia lessons may depend on the visual features of the lessons and the student’s effort and attention skills.
Identification of illegally dumped plastic waste in a highly polluted river in Indonesia using Sentinel-2 satellite imagery
Authors: Sakti Anjar Dimara , Sembiring Emenda , Rohayani Pitri , Fauzan Kamal Nur , Anggraini Tania Septi , Santoso Cokro , Patricia Vinka Aprilla , Ihsan Kalingga Titon Nur , Ramadan Attar Hikmahtiar , Arjasakusuma Sanjiwana , Candra Danang Surya 
Published: March 2023

AbstractPlastic waste monitoring technology based on Earth observation satellites is one approach that is currently under development in various studies. The complexity of land cover and the high human activity around rivers necessitate the development of studies that can improve the accuracy of monitoring plastic waste in river areas. This study aims to identify illegal dumping in a river area using the adjusted plastic index (API) and Sentinel-2 satellite imagery data. Rancamanyar River has been selected as the research area; it is one of the tributaries of Citarum Indonesia and is an open lotic-simple form, oxbow lake type river. Our study is the first attempt to construct an API and random forest machine learning using Sentinel-2 to identify the illegal dumping of plastic waste. The algorithm development integrated the plastic index algorithm with the normalized difference vegetation index (NDVI) and normalized buildup indices. For the validation process, the results of plastic waste image classification based on Pleiades satellite imagery and Unmanned Aerial Vehicle (UAV) photogrammetry was used. The validation results show that the API succeeded in improving the accuracy of identifying plastic waste, which gave a better correlation in the r-value and p-value by + 0.287014 and + 3.76 × 10−26 with Pleiades, and + 0.143131 and + 3.17 × 10−10 with UAV.
The predictive value of the nomogram model of clinical risk factors for ischemia–reperfusion injury after primary percutaneous coronary intervention
Authors: Wang Zuoyan , Peng Jianjun 
Published: March 2023

AbstractIschemia–reperfusion injury is a risk factor for poor clinical prognosis in patients with ST-segment elevation myocardial infarction (STEMI). However, due to the inability to predict the risk of its occurrence early, the effect of intervention measures is still being determined. This study intends to construct a nomogram prediction model and evaluate its value in predicting the risk of ischemia–reperfusion injury (IRI) after primary percutaneous coronary intervention (PCI). The clinical admission data of 386 STEMI patients who underwent primary PCI were retrospectively analyzed. According to the degree of ST-segment resolution (STR), the patients were divided into the STR < 70% group (n = 197) and the STR > 70 group (n = 187). The least absolute shrinkage and selection operator (LASSO) regression method was used to screen out IRI's admission-related clinical risk factors. The R language software was used to construct and verify the IRI nomogram prediction model based on the above indicators. The peak troponin level and the incidence of in-hospital death in the STR < 70% group were significantly higher than those in the STR > 70% group (p < 0.01), and the left ventricular ejection fraction was significantly lower than that in the STR > 70% group (p < 0.01). Combined with the results of LASSO regression and receiver operating characteristic curve comparison analysis, we constructed a six-dimensional nomogram predictive model: hypertension, anterior myocardial infarction, culprit vessel, proximal occlusion, C-reactive protein (CRP) > 3.85 mg/L, white blood cell count, neutrophil cell count, and lymphocyte count. The area under the nomogram's receiver operating characteristic (ROC) curve was 0.779. The clinical decision curve found that the nomogram had good clinical applicability when the occurrence probability of IRI was between 0.23 and 0.95. The nomogram prediction model constructed based on six clinical factors at admission has good prediction efficiency and clinical applicability regarding the risk of IRI after primary PCI in patients with acute myocardial infarction.
Global motion processing in infants’ visual cortex and the emergence of autism
Authors: Hardiansyah Irzam , Nyström Pär , Taylor Mark J. , Bölte Sven , Ronald Angelica , Falck-Ytter Terje 
Published: March 2023

AbstractAutism is a heritable and common neurodevelopmental condition, with behavioural symptoms typically emerging around age 2 to 3 years. Differences in basic perceptual processes have been documented in autistic children and adults. Specifically, data from many experiments suggest links between autism and alterations in global visual motion processing (i.e., when individual motion information is integrated to perceive an overall coherent pattern). Yet, no study has investigated whether a distinctive organization of global motion processing precede the emergence of autistic symptoms in early childhood. Here, using a validated infant electroencephalography (EEG) experimental paradigm, we first establish the normative activation profiles for global form, global motion, local form, and local motion in the visual cortex based on data from two samples of 5-month-old infants (totaln = 473). Further, in a sample of 5-month-olds at elevated likelihood of autism (n = 52), we show that a different topographical organization of global motion processing is associated with autistic symptoms in toddlerhood. These findings advance the understanding of neural organization of infants’ basic visual processing, and its role in the development of autism.