bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Divergence and gene flow history at two large chromosomal inversions involved in long-snouted seahorse ecotype formation”
Authors: Meyer Laura , Barry Pierre , Riquet Florentine , Foote Andrew , Der Sarkissian Clio , Cunha Regina , Arbiol Christine , Cerqueira Frédérique , Desmarais Erick , Bordes Anaïs , Bierne Nicolas , Guinand Bruno , Gagnaire Pierre-Alexandre 
Published: July 2023

AbstractChromosomal inversions can play an important role in divergence and reproductive isolation by building and maintaining distinct allelic combinations between evolutionary lineages. Alternatively, they can take the form of balanced polymorphisms that segregate within populations over time until one arrangement becomes fixed. Many questions remain about how these different inversion polymorphisms arise, how the mechanisms responsible for their long-term maintenance interact, and ultimately how they contribute to speciation. The long-snouted seahorse (Hippocampus guttulatus) is known to be subdivided into partially isolated lineages and marine-lagoon ecotypes differentiated by structural variation. Here, we aim to characterise these differences along the entire genome, and to reconstruct their history and role in ecotype formation. We generated a near chromosome-level reference genome assembly and described genome-wide patterns of diversity and divergence through the analysis of 112 whole-genome sequences from Atlantic, Mediterranean, and Black Sea populations. Combined with linked-read sequencing data, we found evidence for two megabase-scale chromosomal inversions showing contrasted allele frequency patterns across the species range. We reveal that these inversions represent ancient intraspecific polymorphisms, one being likely maintained by divergent selection, and the other by associative overdominance. Haplotype combinations characterising Mediterranean ecotypes also suggest the existence of potential interactions between the two inversions, possibly driven by environment-dependent fitness effects. Lastly, we detected gene flux eroding divergence between inverted alleles at varying levels between the two inversions, with a likely impact on their long-term dynamics.
Human vagus nerve fascicular anatomy: a microCT segmentation and histological study
Authors: Thompson Nicole , Mastitskaya Svetlana , Iacoviello Francesco , Shearing Paul R. , Aristovich Kirill , Holder David 
Published: July 2023

AbstractBackgroundPrevious research has revealed the logical mapping of fascicles in both human somatic and pig vagus nerves, but the organization of fascicles within the human vagus nerve remains largely unknown. Understanding its fascicular arrangement would significantly advance our knowledge of the autonomic nervous system and facilitate studies and application of selective vagus nerve stimulation to avoid off-target effects. The purpose of this study was to trace the thoracic branches of human vagus nerves, investigate their fascicular organization, and analyze the nerves histologically and morphologically.MethodsBoth left and right vagus nerves were dissected from human cadavers, preserving the cardiac, recurrent laryngeal, and pulmonary branches. The nerves were prepared, scanned using microCT, and the fascicles segmented and traced from their branching points. Histology and immunohistochemistry were performed for morphological analysis and validation of the microCT segmentation. The data was then analyzed and compared between nerves.ResultsThe organization of the cardiac, pulmonary, and recurrent laryngeal fascicles was observed for a short distance from their entry point into the nerves. Initially, left vagus nerves showed merging of cardiac and pulmonary fascicles, while the recurrent laryngeal fascicles remained separate. In right vagus nerves, the cardiac fascicles merged with both pulmonary and recurrent laryngeal fascicles. MicroCT imaging limitations prevented visualization and tracing of fiber organization within merged fascicles. Immunohistochemistry and morphological analysis revealed that right vagus nerves were larger and had more fascicles than the left and fascicle counts varied along the nerve, indicating anastomoses. The superior cardiac branch was separate from other fascicles near the VNS cuff placement.ConclusionsIt is possible that organ-specific fibers may still retain some spatial organization despite most fascicles being merged at cervical level. However, fiber tracing andin vivostudies could provide valuable information beyond microCT to resolve this further. The separate superior cardiac fascicles offer potential for targeted neuromodulation of the heart, benefiting conditions like myocardial infarction, heart failure, and atrial fibrillation. Overall, the study provides insights into the morphology and anatomy of human vagus nerves. Our findings thereby contribute to the development of selective vagus nerve stimulation strategies for more precise autonomic regulation.
Synaptogyrin regulates neuronal activity dependent autophagy to degrade synaptic vesicle components and pathological Tau
Authors: Hernandez-Diaz Sergio , Martinez-Olondo Pilar , Sanchez-Mirasierra Irene , Montecinos Carla , Ghimire Saurav , Soukup Sandra-Fausia 
Published: July 2023

AbstractSynapses are specialized neuronal compartments essential for brain communication. Neuronal communication mostly relies on the adequate supply and renovation of synaptic vesicles that fuse with the plasma membrane and release neurotransmitters in response to action potentials. Autophagy is an evolutionary conserved cellular mechanism essential for homeostasis that can be locally regulated in the neuronal synapse. However, the precise mechanisms controlling synaptic autophagy, especially during neuronal communication and pathological scenarios, remain elusive. Here, we report that neuronal activity and amino-acid deprivation regulate synaptic autophagy via distinct molecular mechanisms. We show that Synaptogyrin, a highly abundant presynaptic protein found in synaptic vesicles, is a novel negative regulator of synaptic autophagy in response to neuronal activity without affecting autophagy induction via amino-acid deprivation. We demonstrate that loss of Synaptogyrin modifies the localization of the autophagy protein Atg9 and boosts autophagosome formation at the synapse. Furthermore, activation of synaptic autophagy by loss of Synaptogyrin, but not by amino acid deprivation, leads to the degradation of synaptic vesicle components via autophagy. Reducing the levels of Synaptogyrin results in the degradation of synaptic TAU via autophagy and restores autophagy dysfunction observed in aDrosophilaTau model of Frontotemporal Dementia (FTD). Our data provide novel and valuable information to understand how autophagy is regulated at the synapse in response to neuronal activity and how this process participates in neuronal (dys)function.
Cancer-specific association between Tau (MAPT) and cellular pathways, clinical outcome, and drug response
Authors: Callari Maurizio , Sola Martina , Magrin Claudia , Rinaldi Andrea , Bolis Marco , Paganetti Paolo , Colnaghi Luca , Papin Stéphanie 
Published: July 2023

AbstractTau (MAPT) is a microtubule-associated protein causing common neurodegenerative diseases or inherited frontotemporal lobar degenerations. Emerging evidence for non-canonical functions of Tau in DNA repair and P53 regulation suggests its involvement in cancer. Indeed, preliminary studies have correlated Tau expression with cancer survival or response to therapies. To bring new evidence for a relevant role of Tau in cancer, we carried out anin silicopan-cancer analysis ofMAPTtranscriptomic profile in over 10000 clinical samples from 32 cancer types and over 1300 pre-clinical samples from 28 cancer types provided by the TCGA and the DEPMAP datasets respectively.MAPTexpression associated with key cancer hallmarks including inflammation, proliferation, and epithelial to mesenchymal transition, showing cancer-specific patterns. In some cancer types,MAPTfunctional networks were affected by P53 mutational status. We identified new associations ofMAPTwith clinical outcomes and drug response in a context-specific manner. Overall, our findings indicate that theMAPTgene is a potential major player in multiple types of cancer. Importantly, the impact of Tau on cancer seems to be heavily influenced by the specific cellular environment.
The lncRNA TRG-AS1 promotes the growth of colorectal cancer cells through the regulation of P2RY10/GNA13
Authors: Zhuang Lin , Luo Baoyang , Deng Linghui , Zhang Qi , Li Yuanjiu , Sun Donglin , Zhang Hua , Zhang Qiutao 
Published: July 2023

AbstractPurposeThe lncRNA TRG-AS1 and its co-expressed gene P2RY10 are important for colorectal cancer (CRC) occurrence and development. The purpose of our research was to explore the roles of TRG-AS1 and P2RY10 in CRC progression.MethodsThe abundance of TRG-AS1 and P2RY10 was determined in CRC cell lines. LoVo cells were transfected with si-TRG-AS1 and si-P2RY10 constructs. Subsequently, the viability, colony formation, and migration of the transfected cells were analyzed using cell counting kit-8, clonogenicity, and scratch-wound/Transwell® assays, respectively. Cells overexpressing GNA13 were used to further explore the relationship between TRG-AS1 and P2RY10 along with their downstream functions. Finally, nude mice were injected with different transfected cell types to observe tumor formationin vivo.ResultsTRG-AS1 and P2RY10 were significantly upregulated in HT-29 and LoVo compared to FHC cells. TRG-AS1 knockdown and P2RY10 silencing suppressed the viability, colony formation, and migration of LoVo cells. TRG-AS1 knockdown downregulated the expression of P2RY10, GNA12, and GNA13, while P2RY10 silencing downregulated the expression of TRG-AS1, GNA12, and GNA13. Additionally, GNA13 overexpression reversed the cell growth and gene expression changes in LoVo cells induced by TRG-AS1 knockdown or P2RY10 silencing.In vivoexperiments revealed that CRC tumor growth was suppressed by TRG-AS1 knockdown and P2RY10 silencing.ConclusionsTRG-AS1 knockdown repressed the growth of CRC cells by regulating P2RY10 and GNA13 expression, thereby controlling CRC occurrence and development.
The hippocampus assumes a special role in supporting abstract concept representation
Authors: Kafkas Alex , Mayes Andrew R. , Montaldi Daniela 
Published: July 2023

AbstractUnlike images, words are representational symbols. The associative details inherent within a word’s meaning and the visual imagery it generates, are inextricably connected to the way words are processed and represented. It is well recognised that the hippocampus associatively binds components of a memory to form a lasting representation, and here we show that the hippocampus is especially sensitive to abstract word processing. Using fMRI during recognition we found that the increased abstractness of words produced increased hippocampal activation and that critically this was independent of memory. Interestingly, word recollection produced hippocampal activation independent of word abstractness, while the parahippocampal cortex was sensitive to concrete word representation, independent of memory. We reason that the hippocampus has assumed a critical role in the representation of uncontextualized abstract word meaning, as its information-binding ability allows the retrieval of the semantic and visual associates that, when bound together, generate the abstract concept represented by word symbols. These novel findings not only offer insights for research drawing on word representation, memory, and hippocampal integrity, but critically, they throw important light on how the human brain may have adapted to encode and represent abstract words and concepts as they emerged in our language.
On minimising tumoural growth under treatment resistance
Authors: Fischer Matthias M. , Blüthgen Nils 
Published: July 2023

AbstractDrug resistance is a major challenge for curative cancer treatment, representing the main reason of death in patients. Evolutionary biology suggests pauses between treatment rounds as a way to delay or even avoid resistance emergence. Indeed, this approach has already shown promising preclinical and early clinical results, and stimulated the development of mathematical models for finding optimal treatment protocols. Due to their complexity, however, these models do not lend themself to a rigorous mathematical analysis, hence so far clinical recommendations generally relied on numerical simulations and ad-hoc heuristics. Here, we derive two mathematical models describing tumour growth under genetic and epigenetic treatment resistance, respectively, which are simple enough for a complete analytical investigation. First, we find key differences in response to treatment protocols between the two modes of resistance. Second, we identify the optimal treatment protocol which leads to the largest possible tumour shrinkage rate. Third, we fit the ”epigenetic model” to previously published xenograft experiment data, finding excellent agreement, underscoring the biological validity of our approach. Finally, we use the fitted model to calculate the optimal treatment protocol for this specific experiment, which we demonstrate to cause curative treatment, making it superior to previous approaches which generally aimed at stabilising tumour burden. Overall, our approach underscores the usefulness of simple mathematical models and their analytical examination, and we anticipate our findings to guide future preclinical and, ultimately, clinical research in optimising treatment regimes.
Magicians, unicorns or data cleaners? Exploring the identity narratives and work experiences of data scientists
Authors: Goretzki Lukas , Messner Martin , Wurm Maria 
Published: July 2023

PurposeData science promises new opportunities for organizational decision-making. Data scientists arguably play an important role in this regard and one can even observe a certain “buzz” around this nascent occupation. This paper enquires into how data scientists construct their occupational identity and the challenges they experience when enacting it.Design/methodology/approachBased on semi-structured interviews with data scientists working in different industries, the authors explore how these actors draw on their educational background, work experiences and perception of the contemporary digitalization discourse to craft their occupational identities.FindingsThe authors identify three main components of data scientists’ occupational identity: a scientific mindset, an interest in sophisticated forms of data work and a problem-solving attitude. The authors demonstrate how enacting this identity is sometimes challenged through what data scientists perceive as either too low or too high expectations that managers form towards them. To address those expectations, they engage in outward-facing identity work by carrying out educational work within the organization and (paradoxically) stressing both prestigious and non-prestigious parts of their work to “tame” the ambiguity and hype they perceive in managers’ expectations. In addition, they act upon themselves to better appreciate managers’ perspectives and expectations.Originality/valueThis study contributes to research on data scientists as well as the accounting literature that often refers to data scientists as new competitors for accountants. It cautions scholars and practitioners alike to be careful when discussing the possibilities and limitations of data science concerning advancements in accounting and control.
A decade of integrated reporting studies: state of the art and future research implications
Authors: Songini Lucrezia , Pistoni Anna , Comerio Niccolò , Tettamanzi Patrizia 
Published: July 2023

PurposeOver the past decade, researchers have witnessed an exponential growth in the number of publications on IR. This paper aims to understand the state of the art of the research field and to highlight the areas where further academic research is needed, guiding developments in theory, research, policy and practices.Design/methodology/approachThe authors apply the dynamic literature review method called “Systematic Literature Network Analysis”, which combines systematic literature review and bibliographic network analysis. Furthermore, to overcome some of the limitations connected to the methodology, the authors integrate the literature with a manual content analysis of papers.FindingsIR adoption and practices and their determinants represent the most analyzed aspects of literature. Over time, attention has been paid to more specific issues, such as the relationship between IR and other disclosure mechanisms, IR quality and its assurance, the critical analysis of the IR framework and principles and difficulties in IR adoption. Although the literature on IR can be considered to be in its mature stage, many aspects are still under-researched, so there is plenty of space for future research.Originality/valueThe authors propose the following main issues as subjects to be investigated in future studies: IR is not simply an evolution of sustainability reporting, but an innovative communication tool; the debate on who the recipients of value are (shareholders or stakeholders) and on what the definition of value adopted by IR is still remains an open issue; more attention should be given to the role of IR as a managerial tool, which could support strategy formation and communication, and influence internal processes of performance measurement and evaluation; what the future of IR will be in light of recent EU Corporate Sustainability Reporting Directive and new ISSB's standards is still an open question. From a methodological perspective, little is known about structured approaches in accounting studies. The authors confirm how methodologies, such as that of this paper, may be exploited as a tool to support dynamic analysis for setting the agendas for future studies in the accounting field.
Integrating ISS and SOR models to investigate the determinants of continuance intention toward using m-payment for donations (Sadaqah): the moderating role of Islamic religiosity
Authors: Elsotouhy Mohamed M. , Mobarak Abdelkader M. A. , Dakrory Mona I. , Ghonim Mohamed A. , Khashan Mohamed A. 
Published: July 2023

PurposeDespite the significance of donations (Sadaqah) via mobile payment in Islamic countries, little is known about the variables influencing continuance intention toward using m-payment for donations (Sadaqah). Based on the stimulus-organism-response (S-O-R) model, this research explores the influence of perceived quality (i.e. system, information, service) as a stimulus on customer satisfaction, engagement and delight as organisms, which then affects continuance intention toward using m-payment for donations (Sadaqah) as a response. Moreover, the study investigates the moderating role of Islamic religiosity.Design/methodology/approachUsing partial least squares structural equation modeling (PLS-SEM), a representative data sample of 419 Egyptian Muslims was analyzed to test hypotheses.FindingsThe findings revealed that all perceived quality constructs significantly positively affect customers' satisfaction. Customer satisfaction, in turn, positively affects customer engagement and delight. Moreover, customer engagement, delight and Islamic religiosity significantly positively affect continuance intention toward using m-payment for donations (Sadaqah). The findings also revealed that Islamic religiosity moderates the influence of customer engagement and customer delight on continuance intention toward using m-payment for donations (Sadaqah).Originality/valueThis is the first study to examine using m-payment for donations (Sadaqah) in an Islamic environment based on the S-O-R model.
Negative electricity prices as a signal for lacking flexibility? On the effects of demand flexibility on electricity prices
Authors: Halbrügge Stephanie , Heess Paula , Schott Paul , Weibelzahl Martin 
Published: July 2023

Purpose
The purpose of this paper is to examine how active consumers, i.e. consumers that can inter-temporally shift their load, can influence electricity prices. As demonstrated in this paper, inter-temporal load shifting can induce negative electricity prices, a recurring phenomenon on power exchanges.

Design/methodology/approach
The paper presents a novel electricity-market model assuming a nodal-pricing, energy-only spot market with active consumers. This study formulates an economic equilibrium problem as a linear program and uses an established six-node case study to compare equilibrium prices of a model with inflexible demand to a model with flexible demand of active consumers.

Findings
This study illustrates that temporal coupling of hourly market clearing through load shifting of active consumers can cause negative electricity prices that are not observed in a model with ceteris paribus inflexible demand. In such situations, where compared to the case of inflexible demand more flexibility is available in the system, negative electricity prices signal lower total system costs. These negative prices result from the use of demand flexibility, which, however, cannot be fully exploited due to limited transmission capacities, respectively, loop-flow restrictions.

Originality/value
Literature indicates that negative electricity prices result from lacking flexibility. The results illustrate that active consumers and their additional flexibility can lead to negative electricity prices in temporally coupled markets, which in general contributes to increased system efficiency as well as increased use of renewable energy sources. These findings extend existing research in both the area of energy flexibility and causes for negative electricity prices. Therefore, policymakers should be aware of such (temporal coupling) effects and, e.g. continue to allow negative electricity prices in the future that can serve as investment signals for active consumers.
Safeguarding the rights and welfare of the health-care workers amid the pandemic: a social protection approach
Authors: Abdul Wahab Harlida , Abdul Rahim Asmar , Abdullah Nor Anita 
Published: July 2023

Purpose
This paper aims to study the elements of social protection, namely, the labour market policy (working conditions), social insurance and social assistance from the law and policy standpoints to safeguard the rights and welfare of the frontline health-care workers (HCWs).

Design/methodology/approach
This study applies both doctrinal and non-doctrinal research methods with the legal and authoritative approaches by integrating the three elements of social protections, which are working conditions, insurance protection and social assistance for the protection of HCWs.

Findings
A pragmatic approach to the social protection system by integrating these elements can safeguard the rights and welfare of the frontline HCWs amid the pandemic. This approach should be made effective for the sustainability of the HCW and health industry in Malaysia.

Practical implications
This paper highlights the significance of initiating and empowering ad hoc approaches through the social protection system for the practical and effective protection of frontline HCWs who are the backbone of the nation, in the event of pandemic COVID-19. These practical needs and approaches are pivotal in response to HCWs demands in workplace.

Originality/value
While social protection commonly aims to address disadvantaged groups and to combat poverty, this research adopts the social protection approach with the aims to safeguard the rights and welfare of frontline HCWs amid the COVID-19 pandemic.