bluuebunny/crossref_metadata_2025_split huggingface dataset:
Survival of Salmonella spp. and Pathogenic Escherichia coli in Food Matrixes and Its Relevance in the Development of Proficiency Testing Samples
Authors: Ashish Singh Kumud , Nair Santhini S , Rai Rohit 
Published: January 2023

Abstract
Monitoring of food products by government agencies for their compliance to regulatory limits is an essential step in controlling foodborne outbreaks. For monitoring purposes, an extensive setup of the surveillance system is used, which involves ISO 17025:2017 accredited laboratories for food testing. Participation in proficiency testing (PT) programs is a requirement of ISO 17025:2017, which ensures data accuracy and analyst competency. Participation in PT schemes is costly for laboratories in developing countries as most of the commercial suppliers are situated in the United States and Europe. The literature or data available on creation of microbiological proficiency testing is scanty as much of the data available with commercial suppliers are trade secrets, and there is only 0.06% of research articles available in the Scopus database on the topic. In this review article, an attempt is made to understand the factors impacting the survival of two important foodborne pathogens, i.e., Escherichia coli and Salmonella spp., by extracting information available from growth studies and root-cause analysis of various food safety incidents and recalls. Utilization of this information in the development of PT samples is discussed in this review article along with a focus on the availability of PT samples and associated ISO standards to formulate homogeneous and stable PT samples. This review article elaborates on the focus areas that can be considered by PT providers (PTP)—for example, initial inoculum level and preparation, strain type, microbial growth phase, the impact of different types of food matrixes including low-moisture food, antimicrobial components, pH, presence of competitor microbes, and environmental conditions involving storage temperature, time, and relative humidity. These focus areas can be used to successfully create PT samples by PTP in developing countries.
Undergraduate student attitudes to current poultry industry issues over four semesters: surveying an introductory poultry science course
Authors: Meyer Meaghan M , Bobeck Elizabeth A 
Published: January 2023

Abstract
Individual background and demographics affect student perceptions of animal production. Understanding how science-based education alters these opinions is a critical aspect of improving university instruction as well as increasing consumer engagement in the poultry industry. The study objectives were to quantify the effects of student background, career interests, and science-based instruction on opinions regarding current issues in the poultry industry. Undergraduate students enrolled in a one semester poultry science course at Iowa State University between 2018 and 2021 were anonymously surveyed at the start and end of the semester as part of a 4-yr study. Students who opted to take the survey answered three demographic questions indicating their 1) livestock experience, 2) sex, and 3) career goals. The body of the survey consisted of 16 “poultry issue statements” where students were directed to mark a vertical dash on a 130 mm horizontal line indicating their level of agreement with each statement. Post-survey collection, the line was separated into 5 sections for discussion: responses within 0%–20% indicated strongly disagree, 21%–40% disagree, 41%–60% neutral, 61%–80% agree, and 81%–100% indicated strongly agree. Responses were analyzed using Proc Mixed in SAS Version 9.4 with a Tukey–Kramer adjustment for all pairwise comparisons using main effects including demographic categories, education (pre- or post-instruction), and year the survey was taken. Responses to various issue statements were affected by students’ livestock experience (P &lt; 0.05; 6 out of 16 statements affected), sex (P &lt; 0.05; 5 out of 16 statements), and ultimate career goals (P &lt; 0.05; 4 out of 16 statements). Pre- vs. post-education responses differed significantly in 6 out of 16 statements (P &lt; 0.05), and in 2 out of 16 poultry issue statements, the year of instruction affected student response (P &lt; 0.05). These data indicate that individual student background, sex, and differing career interests impact opinions of current topics in the broiler and layer industries. Further, science-based education as well as the year the course was taken over consecutive semesters significantly altered student opinions.
Effects of replacing corn silage with alfalfa haylage in growing beef cattle diets on performance during the growing and finishing period
Authors: Tarnonsky Federico , Hochmuth Katherine , DiCostanzo Alfredo , DiLorenzo Nicolas 
Published: January 2023

AbstractCorn silage is the predominant mechanically harvested forage source for feedlot cattle production in the United States because of high yield. Alternatively, because of multiple cuttings per year and lower annual cost, the use of alfalfa or other forages, may increase opportunities for manure spreading, perennial soil cover, pollinator habitat, and greater carbon sequestration. The objective of this trial was to determine the feeding value of alfalfa haylage when replacing corn silage in growing cattle diets. One-hundred-sixty-five Angus crossbred steers [326 ± 51 kg of body weight (BW)] were blocked by initial BW and randomly assigned to one of 28 pens at the University of Minnesota feedlot. Pens were randomly assigned to dietary growing treatments. The control diet was comprised of (DM basis) 50% corn silage, 19.25% rolled corn grain, 19.25% high moisture corn, 7% dried distillers grains plus solubles, and 4.5% liquid supplement (corn silage control, CS Control). For alfalfa haylage (AH) diets, AH substituted corn silage at 33% (AH 33), 66% (AH 66), or 100% (AH 100). Growth performance measurements [dry matter intake (DMI), average daily gain (ADG) and gain to feed (G:F) ratio] were assessed for 42 to 70 d depending on BW block. Afterwards, steers were fed a common finishing diet until harvested. There was a linear increase in DMI (P &lt; 0.01) with increasing AH inclusion. Replacing CS with AH linearly decreased (P ≤ 0.05) ADG and G:F. No differences (P ≥ 0.10) were observed in finishing performance or carcass traits. Results from this study demonstrated that greater substitution of corn silage with alfalfa haylage in growing diets resulted in greater intake but reduced rate of gain and gain:feed. Despite slower rate of gain, cattle fed alfalfa haylage at increasing proportions during the growing period were able to compensate in BW gains during the finishing period and reached harvest weight and backfat thickness at similar days on feed than those fed corn silage. Based on these results the energy value of corn silage and alfalfa haylage were 3.05 and 2.39 Mcal ME/kg of DM, respectively, when included at 50% of the diet DM.
Taraxasterol alleviates fatty acid-induced lipid deposition in calf hepatocytes by decreasing ROS production and endoplasmic reticulum stress
Authors: Li Ming , He Yuxin , Zhang Wei , Yin Yufeng , Jiang Qianming , Loor Juan J , Wang Jingjing , Wen Jianan , Yang Wei , Xu Chuang , Zhang Bingbing 
Published: January 2023

AbstractIncreased concentrations of free fatty acids (FFAs) induce reactive oxygen species (ROSs) generation and endoplasmic reticulum (ER) stress, thus, increasing the risk of fatty liver in dairy cows during the periparturient period. In non-ruminants, Taraxasterol (Tara; a pentacyclic triterpenoid found in medicinal plants) plays an important role in anti-inflammatory and antioxidant reactions. Whether Tara can alleviate or prevent fatty liver in ruminants is unknown. We addressed whether Tara supply could dampen lipid accumulation, ROSs production, and ER stress caused by FFAs in calf hepatocytes. Primary calf hepatocytes were isolated from five healthy calves (1 d old, female, 30–40 kg, fasting, rectal temperature 38.7–39.7 °C). In the first experiment, hepatocytes were incubated with various concentrations of Tara (2.5, 5, and 10 μg/mL) for 12 h prior to the 1.2-mM FFAs challenge. Results indicated that the level of ROSs was lowest with 5 μg/mL Tara. Thus, to further characterize the molecular mechanisms whereby Tara protects from FFAs-induced lipid deposition in calf hepatocytes, we performed incubations with 5 μg/mL Tara for 12 h prior to a 1.2-mM FFAs challenge for an additional 12 h. Results indicated that 1.2-mM FFAs challenge increased mitochondrial membrane potential (MMP), enhanced expression of proteins and mRNA associated with ER stress (PERK, IRE1, GRP78, ATF6, and CHOP) and fatty acid synthesis (FASN, ACC1, and SREBP-1c), and ultimately led to increased lipid droplet synthesis. In contrast, Tara treatment alleviated these negative effects after 1.2-mM FFAs challenge. To determine whether Tara protects against FFAs-induced lipid droplet synthesis by alleviating oxidative stress, hepatocytes were treated with 5 μg/mL Tara for 22 h prior to H2O2 (440 μM) challenge for 2 h. Compared with H2O2 treatment alone, results revealed a marked decrease in ROSs, MMP, and protein abundance of ER stress (GRP78, ATF6, and CHOP) and lipid droplet synthesis in response to Tara prior to H2O2 challenge. Data suggested that the increase in mitochondrial ROSs production contributes to lipid accumulation in calf hepatocytes. Collectively, our in vitro data indicate that Tara alleviates fatty acid-induced lipid deposition. Further research is warranted to ascertain that Tara can be helpful in the therapeutic management of early lactating cows to control or alleviate excessive hepatic lipid deposition.
Integrating Palliative Care into Burn Care: A Retrospective Application of Criteria for Appropriate Referral
Authors: Grossoehme Daniel H , Bish Brianna M , Lou Richard , Khandelwal Anjay , Brown Miraides , Brown Carrie , Teo Esther , Friebert Sarah 
Published: January 2023

Abstract
Burn injuries often require complex interdisciplinary care. Despite potential benefits, consultation with specialist palliative care typically occurs in 2% of burn admissions, due largely to lack of availability and misconceptions concerning palliative care. Criteria to guide consultation with specialist palliative care have been developed at this institution. The purpose of this study was to quantify the sensitivity and specificity of these criteria, and then optimize them. Retrospective examination of 388 admissions over 15 months at this adult and pediatric Burn Center were conducted. Chi-square or Fisher’s exact test for categorical variables and Wilcoxon Rank Sum test for continuous variables were used to test for between-group differences in demographic and clinical characteristics. Logistic regression model was built to determine modified Baux cut-off score using Youden index (J) value. Criteria were sensitive (0.96) and specific (0.90), though with a low positive predictive value (0.43). Persons with lengths of stay greater than one week, advanced directives, Allow Natural Death orders, and/or deterioration events were significantly more likely to have had palliative care consulted. There were significant between-group differences (with/without palliative care consultation) based on burn surface area, modified Baux score, and mortality risk. Potential criteria revisions are proposed. Prospective adherence to revised criteria could provide useful guidance to providers and benefit to patients and families.
Alginate and Nanocellulose Dressings With Extract From Salmon Roe Reduce Inflammation and Accelerate Healing of Porcine Burn Wounds
Authors: Gilljam Karin M , Stenlund Patrik , Standoft Simon , Andersen Sisse Bindslev , Kaaber Kari , Lund Henrik , Bryn Karl R K 
Published: January 2023

Abstract
Partial-thickness thermal burn wounds are characterized by a prolonged inflammatory response, oxidative stress, tissue damage, and secondary necrosis. An optimal dressing for burn wounds would reduce inflammation and oxidative stress while providing a moist, absorbent, and protective cover. We have developed an extract from unfertilized salmon roe containing components with potential anti-inflammatory and antioxidative properties, called HTX. HTX has been combined with alginate from brown algae and nanocellulose from tunicates, and 3D printed into a solid hydrogel wound dressing called Collex. Here, Collex was tested on partial thickness burn wounds in Göttingen minipigs compared to Jelonet, and a variant of Collex without HTX. We found that dermal treatment of burn wounds with Collex resulted in accelerated healing at a majority of measured points over 23 days, compared to treatment with Jelonet. In comparison to Collex without HTX, Collex enhanced healing in the first week after trauma where wound progression was pronounced. Notably, Collex reduced the inflammatory response in the early post-injury phase. The anti-inflammatory response of Collex was investigated in more detail on activated M1 macrophages. We found that Collex, as well as HTX alone, significantly reduced the secretion of pro-inflammatory interleukin-1β as well as intracellular levels of oxidative stress. The results from this study indicate that Collex is a potent dressing for the treatment of burn wounds, with the anti-inflammatory effect of HTX beneficial in the initial phase, and the moist qualities of the hydrogel favorable both in the initial and the proceeding proliferative phase of wound healing.
How Do Consumers Read and Encode a Price?
Authors: Laurent Gilles , Vanhuele Marc 
Published: January 2023

Abstract
Do consumers really read a price from left to right, as assumed in past research? Or does price reading operate like word reading, with a single fixation toward the middle? Three eye-tracking lab studies reject both theories, revealing instead a distinct reading pattern: multiple fixations, with the first located on average between the first third and middle of the price; the first eye movement is usually to the left; and subsequent eye movements are as often to the left as to the right. Overall, consumers pay as much attention to cents as euros, with the cents part influencing how prices are encoded in memory, as evidenced by an in-store price-recall survey. The reading process identifies whether to encode a price verbally as is or replace it with a shorter substitute that is easier to memorize and turns out to be well correlated with the actual price (r = 0.952). When consumers compare two prices, eye movements and the subsequent subjective estimation of the price difference depend on whether or not the prices have identical integer parts. The combined findings of four studies suggest that consumers have developed a reliable, efficient ability to read and encode prices, despite limitations of their visual span and working memory.
Allocation of Institutional Responsibility for Climate Change Mitigation: Judicial Application of Constitutional Environmental Provisions in the European Climate Cases Arctic Oil, Neubauer, and l’Affaire du siècle
Authors: Hellner Agnes , Epstein Yaffa 
Published: January 2023

Abstract
This article examines three constitutional environmental provisions and how they have been applied by courts in Europe in three climate cases from Norway, Germany and France. In each of these cases, directive principles, that is, constitutionally entrenched state obligations to protect social values, generally by enacting legislation, played a key role in judicial decisions regarding climate change mitigation. We engage with Lael K. Weis’s analytical framework on directive principles to clarify the allocation of institutional responsibility for climate change mitigation as applied in these three cases, and argue that clarifying these roles alleviates some of the criticism regarding the democratic legitimacy of judicial decision making on climate change. Importantly, while courts do not directly enforce these types of constitutional directive principles, they must adjudicate them. When courts interpret constitutionally mandated legislation in light of directive principles, they develop new constitutional environmental norms. While most scholarly analysis of environmental constitutionalism has focused on environmental rights, our examination confirms Weis’s thesis that directive principles aimed at legislatures are also important forms of environmental constitutionalism, and deserving of further attention.
Predictors of genomic diversity within North American squamates
Authors: Larkin Ivy E , Myers Edward A , Carstens Bryan C , Barrow Lisa N 
Published: January 2023

AbstractComparisons of intraspecific genetic diversity across species can reveal the roles of geography, ecology, and life history in shaping biodiversity. The wide availability of mitochondrial DNA (mtDNA) sequences in open-access databases makes this marker practical for conducting analyses across several species in a common framework, but patterns may not be representative of overall species diversity. Here, we gather new and existing mtDNA sequences and genome-wide nuclear data (genotyping-by-sequencing; GBS) for 30 North American squamate species sampled in the Southeastern and Southwestern United States. We estimated mtDNA nucleotide diversity for 2 mtDNA genes, COI (22 species alignments; average 16 sequences) and cytb (22 species; average 58 sequences), as well as nuclear heterozygosity and nucleotide diversity from GBS data for 118 individuals (30 species; 4 individuals and 6,820 to 44,309 loci per species). We showed that nuclear genomic diversity estimates were highly consistent across individuals for some species, while other species showed large differences depending on the locality sampled. Range size was positively correlated with both cytb diversity (phylogenetically independent contrasts: R2 = 0.31, P = 0.007) and GBS diversity (R2 = 0.21; P = 0.006), while other predictors differed across the top models for each dataset. Mitochondrial and nuclear diversity estimates were not correlated within species, although sampling differences in the data available made these datasets difficult to compare. Further study of mtDNA and nuclear diversity sampled across species’ ranges is needed to evaluate the roles of geography and life history in structuring diversity across a variety of taxonomic groups.
CRISPR/Cas9-induced Mutation ofSex Peptide ReceptorGeneBdsprAffects Ovary, Egg Laying, and Female Fecundity inBactrocera dorsalis(Hendel) (Diptera: Tephritidae)
Authors: Chen Hao , Sun Hao , Xie Junfei , Yao Zhichao , Zheng Wenping , Li Ziniu , Deng Zhurong , Li Xiaoxue , Zhang Hongyu 
Published: January 2023

AbstractThe oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), is an invasive and polyphagous pest of horticultural crops, and it can cause huge economic losses in agricultural production. The rapid development of CRISPR/Cas9 gene editing technology has provided new opportunities for the scientific control of agricultural pests. Here, we explore the applicability of the B. dorsalis sex peptide receptor (Bdspr) as a target gene for the CRISPR/Cas9-based sterile insect technique (SIT) in B. dorsalis. We screened two high-efficient single guide RNAs (sgRNAs) for gene editing. The results showed that both mutation efficiency and germline transmission rate were 100% in the surviving G0 females (8/8) from injected embryos, and that 75% of mosaically mutated G0 females (6/8) were sterile. The 50% of heterozygous G1 females (4/8) could not lay eggs; 100% of eggs laid by them could not survive; and 62.5% of individual females (5/8) had abnormal ovaries. These results indicate that Bdspr plays an important role in regulating fertility, egg viability, and ovary development in female B. dorsalis, suggesting that the spr gene can be used for CRISPR/Cas9-based SIT in B. dorsalis.
Gastrointestinal tract metastasis with subsequent intussusception and obstruction from an invasive lobular breast cancer: a case report
Authors: Katuwal Binit , Morin Donald , Kolachalam Ramachandra 
Published: January 2023

Abstract
Breast cancer is the commonest cancer in female population with lobular subtype comprising about 10% of all breast cancers. Breast cancer metastasis occurs in 0.3–18% of patients, with lobular cancer again being the most common subtype. We present an 85-year-old female with previous history of right breast lobular carcinoma in situ (LCIS), who was diagnosed to have lobular carcinoma of breast metastasising to stomach after 10 years of initial diagnosis. After 2 years, the patient was found to have metastasis to the terminal ileum and caecum causing intussusception, which led to obstruction. The patient’s primary LCIS was estrogen receptor (ER) positive, progesterone receptor (PR) negative and Her2neu negative, which correlated with both the gastric and ileocecal lesions. The gastric and ileocecal masses both were positive for CK7 and GATA 3 and negative for E-cadherin and CD20. Detailed morphological and immunohistochemical analysis can differentiate primary lobular cancer of the gastrointestinal tract from metastatic cancer.
An Aramaic Verb Form in a Neo-Babylonian Letter
Authors: Berzon Ekaterina , Kalinin Maksim , Koval Sergey , Loesov Sergey 
Published: January 2023

Abstract
This paper offers an interpretation of an insufficiently understood verb form in the Neo-Babylonian letter OIP 114, 17:8, 29, thereby clarifying its contents. The word in question is shown to be an Aramaic verb form. This interpretation is supported by observations on the orthography and phonology of early Neo-Babylonian. It follows from the study that in eighth-century Neo-Babylonian two glottalized (‘emphatic’) consonants in the same word still underwent dissimilation, as in the second millennium bce (Geers’ law). The paper also demonstrates that, against communis opinio, erstwhile Aramaic fricative interdentals had shifted to stops by the eighth century bce, while the orthography of contemporaneous Aramaic alphabetic texts reflected an earlier stage of language evolution. This conclusion has been reached by means of comparing cuneiform renderings of proto-Semitic interdentals in West Semitic personal names in eighteenth-century bce Old Babylonian texts and in Neo-Babylonian texts from the ninth and eighth centuries bce.
Belowground carbon allocation, root trait plasticity, and productivity during drought and warming in a pasture grass
Authors: Chandregowda Manjunatha H , Tjoelker Mark G , Pendall Elise , Zhang Haiyang , Churchill Amber C , Power Sally A 
Published: January 2023

Abstract
Sustaining grassland production in a changing climate requires an understanding of plant adaptation strategies, including trait plasticity under warmer and drier conditions. However, our knowledge to date disproportionately relies on aboveground responses, despite the importance of belowground traits in maintaining aboveground growth, especially in grazed systems. We subjected a perennial pasture grass, Festuca arundinacea, to year-round warming (+3 °C) and cool-season drought (60% rainfall reduction) in a factorial field experiment to test the hypotheses that: (i) drought and warming increase carbon allocation belowground and shift root traits towards greater resource acquisition and (ii) increased belowground carbon reserves support post-drought aboveground recovery. Drought and warming reduced plant production and biomass allocation belowground. Drought increased specific root length and reduced root diameter in warmed plots but increased root starch concentrations under ambient temperature. Higher diameter and soluble sugar concentrations of roots and starch storage in crowns explained aboveground production under climate extremes. However, the lack of association between post-drought aboveground biomass and belowground carbon and nitrogen reserves contrasted with our predictions. These findings demonstrate that root trait plasticity and belowground carbon reserves play a key role in aboveground production during climate stress, helping predict pasture responses and inform management decisions under future climates.