bluuebunny/crossref_metadata_2025_split huggingface dataset:
OCUPACIÓN DE LA COMUNIDAD DE BÚHOS EN UN BOSQUE TEMPLADO EN MEXICO
Authors: Fernández-Martínez Eladio C. , Enríquez Paula L. 
Published: February 2023

Los bosques templados son de los ecosistemas más amenazados. Las alteraciones en los bosques modifican la estructura de la vegetación y provoca cambios en la distribución y abundancia de las especies. Los búhos son elementos importantes en los bosques y estudiar su ocupación permitirá entender su abundancia y las variables ambientales asociadas. Para estimar los índices de ocupación de cinco especies de búhos en el área, se recorrieron tres transectos lineales en 2012 y 2015 en el Área Natural Protegida Piedra Canteada, Tlaxcala México. Asimismo se establecieron parcelas para medir variables estructurales de la vegetación y fisiográficas. Tres especies presentaron mayores índices de ocupación en ambos años: Aegolius acadicus; Psiloscops flammeolus y Bubo virginianus. Mientras que Tyto alba y Megascops kennicottii presentaron menores índices de ocupación. Hubo variables asociadas a los índices de ocupación de B. virginianus como el número de troncos caídos y a la fuente de agua más cercana. Las variables de los modelos de ocupación se asociaron con el disturbio de la vegetación, las distancias a las fuentes de agua y a asentamientos humanos. Estos resultados proveen la primera aproximación a los patrones de ocupación y asociación a las variables de la vegetación de las especies de búhos en bosques templados en el noroeste de Tlaxcala, México.
UN RARO CASO DE ENCANECIMIENTO PROGRESIVO CON EXPRESIÓN DE XANTOCROISMO EN EL CACIQUE MEXICANO (CASSICULUS MELANICTERUS)
Authors: Ruiz Erick Ruben Rodriguez 
Published: February 2023

Resumen. Con el objeto de reportar un raro caso de encanecimiento progresivo con expresión de xantocroísmo en el cacique mexicano (Caciculus melanicterus) y su desarrollo en el tiempo, se tomaron fotografías anualmente entre 2018 y 2021 a un individuo de plumaje aberrante. En 2018 el ave mostraba simplemente una macha amarilla atípica en la cabeza que ocupaba un estimado del 5% de la superficie de su plumaje. Con el tiempo el color negro de sus plumas fue progresivamente sustituido por amarillo con ligera despigmentación en la cara y las alas. De manera que en 2019, la aberración se había extendido hasta abarcar aproximadamente el 50% de su plumaje y para 2020-2021 alcanzó el 75%. El presente reporte se trata del primer caso de este tipo conocido en el Neotrópico.Palabras clave: Aberración del plumaje, carotenismo, flavismo, pluma, xantocroismo.
Analyse d’une démarche d’accompagnement de jeunes pères marginalisés
Authors: Charlebois François-Xavier 
Published: February 2023

Cet article traite d’une étude de cas réalisée dans le cadre d’une recherche-action en milieu communautaire au Québec. Cette étude a pour objet une démarche autobiographique en petit groupe menée avec des jeunes hommes marginalisés sur les thèmes de la masculinité et de la paternité. La démarche est discutée à partir du concept d’accompagnement éducatif afin de dégager une analyse critique des pratiques d’intervention sociale dites de proximité. Trois limites et enjeux éthiques liés à la proximité sont identifiés et discutés : la séduction, la juste distance et les limites des activités informelles.
Contrer la violence domestique faite aux femmes au Brésil par l’intervention interdisciplinaire et socioéducative
Authors: Soares Bidarra Zelimar , Alves Camila Kelly , Rohde Luana , Veiss Villian , Lalande Célyne , Lessard Geneviève , Auger Louis-Philippe 
Published: February 2023

Cet article présente la démarche ayant donné lieu à la mise en oeuvre d´un programme interdisciplinaire et socioéducatif novateur au Brésil pour contrer la violence domestique : le Numape-Toledo, situé dans la province du Paraná. L’article décrit en premier lieu l’évolution historique au Brésil ayant mené à la reconnaissance de la problématique de la violence de genre. Puis, l’organisme Numape-Toledo est présenté en mettant en lumière l’originalité des services qui y sont offerts. Enfin, les bénéfices et les enjeux rencontrés par l’organisme sont discutés en vue d’alimenter plus largement les stratégies préventives et les interventions auprès des femmes violentées.
Phytochemical Screening and Antimicrobial Activity of Sarcocephalus latifolius Smith Roots Extracts
Authors: Chabi-Sika Kamirou , Sina Haziz , Boya Bawa , Mama-Sirou Ibrahima , Kpangon Lucas , Salami Hafiz A. , Kelomey Aude , Roko Gautier , Assogba Sylvestre A. , Adoko Marcel Y. , Baba-Moussa Lamine 
Published: February 2023

Aims: This work aims to evaluate the antimicrobial activity of Sarcocephalus latifolius extracts.
Methodology: Thus, phytochemical screening was qualitatively accessed using colorations or precipitations methods. Ethanolic and aqueous extracts were used to evaluate the antimicrobial activity. The antimicrobial activity, using the diffusion method, was evaluated on eight strains including two reference strains (Streptococcus pneumoniae ATCC 49619 and Pseudomonas aeruginosa ATCC 27853) and six clinically isolated S. pneumoniae and P. aeruginosa strains. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined by the microdilution method.
Results: The phytochemical screening showed the presence of flavonoids, anthocyanins, mucilages, saponosides, C-heterosides and O-heterosides. Antimicrobial activity showed that the ethanolic extract with the lowest MIC (1.25 mg/ml) inhibited reference strains (S. pneumoniae ATCC 49619 and P. aeruginosa ATCC 27853) and clinical isolated S. pneumoniae and P. aeruginosa strains. The largest inhibition diameter (19± 1.33) was obtained with the ethanolic extract against clinical isolated Pseudomonas aeruginosa and (15.5± 1) against the reference one. The aqueous extract inhibited only reference strains.
Conclusions: The data of this study indicate that the extracts of S. latifolius present antimicrobial properties. This may justify its traditional use in the treatment of microbial infections.
Synergistic Association of Hepatitis B Surface Antigen and Plasma Epstein-Barr Virus DNA Load on Distant Metastasis in Patients With Nasopharyngeal Carcinoma
Authors: Li Haojiang , Cao Di , Li Shuqi , Chen Binghong , Zhang Yun , Zhu Yuliang , Luo Chao , Lin Weiqun , Huang Wenjie , Ruan Guangying , Zhang Rong , Li Jiang , Liu Lizhi 
Published: February 2023

ImportanceHepatitis B surface antigen (HBsAg) reportedly increases the risk of distant metastasis among patients with nasopharyngeal carcinoma (NPC). However, the associated potential interaction and changes in hazard ratios (HRs) between HBsAg and different plasma Epstein-Barr (EBV) DNA levels are unknown. Moreover, the potential HBsAg-positive–associated NPC metastatic mechanism remains unclear.ObjectiveTo investigate the prognostic value and biological associations of HBsAg and plasma EBV DNA levels on distant metastasis in patients with NPC.Design, Setting, and ParticipantsRetrospective cohort study performed at Sun Yat-sen University Cancer Center between January 2010 and January 2013. A total of 792 patients with nonmetastatic NPC were enrolled. The median (range) follow-up time was 62.1 (1.4-83.4) months. Of these patients, 17.8% presented with HBsAg positivity. Cytological experiments were performed to evaluate the role of HBsAg in the invasion and migration of EBV-positive NPC cells. Data analysis was performed from July 2020 to April 2021.Main Outcomes and MeasuresThe primary end point was distant metastasis–free survival. Association rules were used to identify new rules related to distant metastasis. Interaction plots, univariate and multivariate Cox regression analyses, stratification analysis, and quantification using HRs were conducted. Additionally, cell migration and invasion assays, as well as Western blotting, were performed in the cytological validation.ResultsAmong the 792 patients, 576 (72.7%) were male, with a median (IQR) age of 45 (38-53) years. The HBsAg-positive group exhibited a significant interaction and increased risk of distant metastasis when plasma EBV DNA cutoff levels were 1.5 × 1000 copies/mL or greater. The HR was 9.16 (95% CI, 2.46-34.14) when the plasma EBV DNA load reached 6 × 1000 copies/mL, which was higher than that in patients with stage IV disease (HR, 2.01; 95% CI, 1.13-3.56; P = .02). In cytological experiments, HBsAg promoted epithelial-mesenchymal transition by upregulating vimentin and fibronectin in EBV-positive NPC cells in vitro, thereby promoting invasion and migration of EBV-positive NPC cells.Conclusions and RelevanceIn this cohort study, the observed synergistic association between HBsAg and plasma EBV DNA load represented a novel potential mechanism underlying the increased risk of distant metastasis in patients with NPC. Hence, attention should be paid to patients with NPC with HBsAg positivity, especially when the plasma EBV DNA level is 6 × 1000 copies/mL or greater. Consideration of this synergistic association will contribute to more accurate individualized management.
Association of Recent SARS-CoV-2 Infection With New-Onset Alcohol Use Disorder, January 2020 Through January 2022
Authors: Olaker Veronica R. , Kendall Ellen K. , Wang Christina X. , Parran Theodore V. , Terebuh Pauline , Kaelber David C. , Xu Rong , Davis Pamela B. 
Published: February 2023

ImportanceThe COVID-19 pandemic affects many diseases, including alcohol use disorders (AUDs). As the pandemic evolves, understanding the association of a new diagnosis of AUD with COVID-19 over time is required to mitigate negative consequences.ObjectiveTo examine the association of COVID-19 infection with new diagnosis of AUD over time from January 2020 through January 2022.Design, Setting, and ParticipantsIn this retrospective cohort study of electronic health records of US patients 12 years of age or older, new diagnoses of AUD were compared between patients with COVID-19 and patients with other respiratory infections who had never had COVID-19 by 3-month intervals from January 20, 2020, through January 27, 2022.ExposuresSARS-CoV-2 infection or non–SARS-CoV-2 respiratory infection.Main Outcomes and MeasuresNew diagnoses of AUD were compared in COVID-19 and propensity score–matched control cohorts by hazard ratios (HRs) and 95% CIs from either 14 days to 3 months or 3 to 6 months after the index event.ResultsThis study comprised 1 201 082 patients with COVID-19 (56.9% female patients; 65.7% White; mean [SD] age at index, 46.2 [18.9] years) and 1 620 100 patients with other respiratory infections who had never had COVID-19 (60.4% female patients; 71.1% White; mean [SD] age at index, 44.5 [20.6] years). There was a significantly increased risk of a new diagnosis of AUD in the 3 months after COVID-19 was contracted during the first 3 months of the pandemic (block 1) compared with control cohorts (HR, 2.53 [95% CI, 1.82-3.51]), but the risk decreased to nonsignificance in the next 3 time blocks (April 2020 to January 2021). The risk for AUD diagnosis increased after infection in January to April 2021 (HR, 1.30 [95% CI, 1.08-1.56]) and April to July 2021 (HR, 1.80 [95% CI, 1.47-2.21]). The result became nonsignificant again in blocks 7 and 8 (COVID-19 diagnosis between July 2021 and January 2022). A similar temporal pattern was seen for new diagnosis of AUD 3 to 6 months after infection with COVID-19 vs control index events.Conclusions and RelevanceElevated risk for AUD after COVID-19 infection compared with non–COVID-19 respiratory infections during some time frames may suggest an association of SARS-CoV-2 infection with the pandemic-associated increase in AUD. However, the lack of excess hazard in most time blocks makes it likely that the circumstances surrounding the pandemic and the fear and anxiety they created also were important factors associated with new diagnoses of AUD.
Cryopreservation: A Comprehensive Overview, Challenges, and Future Perspectives
Authors: Parihar Arpana , Kumar Avinash , Panda Udwesh , Khan Rukhsar , Parihar Dipesh Singh , Khan Raju 
Published: February 2023

AbstractCryopreservation is the most prevalent method of long‐term cell preservation. Effective cell cryopreservation depends on freezing, adequate storage, and correct thawing techniques. Recent advances in cryopreservation techniques minimize the cellular damage which occurs while processing samples. This article focuses on the fundamentals of cryopreservation techniques and how they can be implemented in a variety of clinical settings. The article presents a brief description of each of the standard cryopreservation procedures, such as slow freezing and vitrification. Alongside that, the membrane permeating and nonpermeating cryoprotectants are briefly discussed, along with current advancements in the field of cryopreservation and variables influencing the cryopreservation process. The diminution of cryoinjury incurred by the cell via the resuscitation process will also be highlighted. In the end application of cryopreservation techniques in many fields, with a special emphasis on stem cell preservation techniques and current advancements presented. Furthermore, the challenges while implementing cryopreservation and the futuristic scope of the fields are illustrated herein. The content of this review sheds light on various ways to enhance the output of the cell preservation process and minimize cryoinjury while improving cell revival.
Sintered Polycrystalline BiVO4 Pellet for Stable X‐Ray Detector with Low Detection Limit
Authors: Jia Shanshan , Xiao Yingrui , Bu Nuo , Li Nan , Li Deng , Yang Zhou , Liu Shengzhong (Frank) 
Published: February 2023

AbstractSemiconductors based on Bi element show large attenuation coefficients to X‐ray photons and have been recognized as candidates for X‐ray detectors. However, the application of stable Bi‐based oxide materials to X‐ray detectors has been rarely investigated. In this research, the X‐ray response of a BiVO4 pellet has been studied. It has been found that the BiVO4 pellet has a large resistivity of 1.3 × 1012 Ω cm, negligible current drift of 6.18 × 10−8 nA cm−1 s−1 V−1 under electrical bias and mobility lifetime product, µτ, of 1.75 × 10−4 cm2 V−1, which renders the pellet with an X‐ray sensitivity of 241.3 µC Gyair−1 cm−2 and a detection limit of 62 nGyair s−1 under 40 KVp X‐ray illumination and 40 V bias voltage. The BiVO4 pellet also shows operational stability under steady X‐ray illumination with total dose of 2.01 Gyair, equal to the dose of 20 000 medical chest X‐ray inspections. This research reveals the potential application of BiVO4 in X‐ray detection devices and inspires further research in this area.
Customized Optical Fiber Birefringent Sensors to Multipoint and Simultaneous Temperature and Radial Strain Tracking of Lithium‐Ion Batteries
Authors: Matuck Lucca C. , Cabrita Pedro D. , Pinto João L. , Marques Carlos A. , Nascimento Micael S. 
Published: February 2023

AbstractBatteries are seen as a vital piece for actual carbon‐neutral world needs. However, as they basically operate through extremely exothermic electrochemical events, special attention must be paid to their thermal and volumetric constraints, where continuous and accurate monitoring is of great importance. Considering it, customized optical sensors, based on fiber Bragg gratings recorded in birefringent PANDA fibers (FBG‐PANDA), are developed and instrumented in a coiled configuration into a cylindrical Li‐ion battery (LiB) to simultaneously track temperature and radial strain during galvanostatic cycles, in three different battery locations, for the first time. The maximum temperature and radial strain variations are achieved in the middle and at the end of the discharge steps. This thermal behavior is correlated with the internal heat generation and transfer of thermal energy processes, with a tendency to accumulate in the LiB central zone.Good feasibility and reproducibility are observed in the optical sensor's performance and demonstrated that by operating as a multiparameter decouple system, they can decrease the complexity and intrusiveness in batteries, by using only one optical fiber line. The reported results are in accordance with the literature, indicating that such sensors could be consistently used for future applications regarding battery safety parameters sensing.
Numerical Investigation of Flow Patterns and Mixing Characteristics in a 3D Micromixer with Helical Elements over Wide Reynolds Numbers
Authors: Liu Bo , Chen Chaozhan , Ran Bin , Shi Liuyong , Wei Jiashen , Jin Jing , Zhu Yonggang 
Published: February 2023

AbstractMicromixers play an important role in the micro total analysis systems (µTAS) that require rapid and effective mixing. However, current micromixers are usually designed to meet the need for mixing at limited Reynolds numbers. Herein, this paper presents a high‐performance 3D micromixer with helical elements over wide Reynolds numbers to achieve efficient mixing and has numerically investigated flow patterns and mixing characteristics accordingly. A coupled numerical model is built to analyze the flow pattern, mixing behavior, residence time distribution (RTD), and mixing performance of the 3D micromixer. Helical elements inside could greatly enhance a secondary flow and induce chaotic advection around. Dean vortices are observed in the micromixer, enormously shortening the RTD and promoting the related mixing effect. Furthermore, the effects of various geometric parameters are systematically investigated to optimize the performance of this 3D micromixer. The optimized micromixer shows excellent mixing ability over wide Reynolds numbers ranging from 0.01 to 2333.3, with an efficiency of over 94%. In addition, the numerical results are proved well consistent with analytical and experimental data correspondingly. Therefore, this work would potentially expand the use scope of 3D micromixers and provide a constructive strategy to develop essential parts involving the mixing or reacting process in µTAS.
Nanomodified Switch Induced Precise and Moderate Activation of CAR‐T Cells for Solid Tumors
Authors: Wang Xinyue , Meng Fanyan , Li Xiang , Xue Luxin , Chen Anni , Qiu Yuling , Zhang Zhifan , Li Lin , Liu Fengcen , Li Yishan , Sun Zhichen , Chu Yanhong , Xu Ruihan , Yu Lixia , Shao Jie , Tian Manman , Qian Xiaoping , Liu Qin , Liu Baorui , Li Rutian 
Published: February 2023

AbstractChimeric antigen receptor (CAR)‐T cell therapy is a transformative treatment against advanced malignancies. Unfortunately, once administrated in vivo, CAR‐T cells become out of artificial control, and fierce response to CAR‐T therapy may cause severe adverse events, represented by cytokine‐release syndrome and on‐target/off‐tumor effects. Here, a nanomodified switch strategy is developed, leading to sustained and precise “on‐tumor only” activation of CAR‐T cells. Here, original gelatinase‐responsive nanoparticles (NPs) are used to selectively deliver the heterodimerizing switch, which is the key component of switchable CAR with separated activation modules. The “NanoSwitch” is tumor‐specific, thus inactivated switchable CAR‐T cells do little harm to normal cells, even if the normal cells express the target of CAR‐T. Owing to the sustained‐release effect of NPs, the CAR‐T cells are activated smoothly, avoiding sudden release of cytokine. These data introduce NanoSwitch as a universal and applicable solution to safety problems of CAR‐T therapy regardless of the target.
LYRM7‐associated mitochondrial complex III deficiency with non‐cavitating leukoencephalopathy and stroke‐like episodes
Authors: Alfattal Rita , Alfarhan Maryam , Algaith Adeeb M. , Albash Buthaina , Elshafie Reem M. , Alshammari Asma , Alahmad Ahmad , Dashti Fatima , Alsafi Rasha , Alsharhan Hind 
Published: February 2023

AbstractDefects of respiratory chain complex III (CIII) result in characteristic but rare mitochondrial disorders associated with distinct neuroradiological findings. The underlying molecular defects affecting mitochondrial CIII assembly factors are few and yet to be identified. LYRM7 assembly factor is required for proper CIII assembly where it acts as a chaperone for the Rieske iron–sulfur (UQCRFS1) protein in the mitochondrial matrix and stabilizing it. We present here the seventeenth individual with LYRM7‐associated mitochondrial leukoencephalopathy harboring a previously reported rare pathogenic homozygous LYRM 7 variant, c.2T>C, (p.Met1?). Like previously reported individuals, our 5‐year‐old male proband presented with recurrent metabolic and lactic acidosis, encephalopathy, and fatigue. Further, he has additional, previously unreported features, including an acute stroke like episode with bilateral central blindness and optic neuropathy, recurrent hyperglycemia and hypertension associated with metabolic crisis. However, he has no signs of psychomotor regression. He has been stable clinically with residual left‐sided reduced visual acuity and amblyopia, and no more metabolic crises for 2‐year‐period while on the mitochondrial cocktail. Although the reported brain MRI findings in other affected individuals are homogenous, it is slightly different in our index, revealing evidence of bilateral almost symmetric multifocal periventricular T2 hyperintensities with hyperintensities of the optic nerves, optic chiasm, and corona radiata but with no cavitation or cystic changes. This report describes new clinical and radiological findings of LYRM7‐associated disease. The report also summarizes the clinical and molecular data of previously reported individuals describing the full phenotypic spectrum.
Microwave‐assisted synthesis of blue fluorescent molybdenum nanoclusters with maltose‐cysteine Schiff base for detection of myoglobin and γ‐aminobutyric acid in biofluids
Authors: Harshita  , Park Tae‐Jung , Kailasa Suresh Kumar 
Published: February 2023

AbstractThe fabrication of stable fluorescent MoNCs (molybdenum nanoclusters) in aqueous media is quite challenging as it is not much explored yet. Herein, we report a facile and efficient strategy for fabricating MoNCs using 2,3 dialdehyde maltose‐cysteine Schiff base (DAM‐cysteine) as a ligand for detecting myoglobin and γ‐aminobutyric acid (GABA) in biofluids with high selectivity and sensitivity. The DAM‐cysteine‐MoNCs displayed fluorescence of bright blue color under a UV light at 365 nm with an emission peak at 444 nm after excitation at 370 nm. The synthesized DAM‐cysteine‐MoNCs were homogeneously distributed with a mean size of 2.01 ± 0.98 nm as confirmed by the high‐resolution transmission electron microscopy (HR‐TEM). Further, X‐ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FT‐IR) techniques were utilized to confirm the elemental oxidation states and surface functional groups of the DAM‐cysteine‐MoNCs. After the addition of myoglobin and GABA, the emission peak of DAM‐cysteine‐MoNCs at 444 nm was significantly quenched. This resulted in the development of a quantitative assay for the detection of myoglobin (0.1–0.5 μM) and GABA (0.125–2.5 μM) with the lower limit of detection as 56.48 and 112.75 nM for myoglobin and GABA, respectively.