bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of coronavirus disease 2019 vaccination on seizures in patients with epilepsy
Authors: Fang Xiqin , Qiao Shan , Zhang Ranran , Yang Tingting , Wang Zhihao , Kong Qingxia , Sun Meihua , Geng Jianhong , Fang Chunyan , Chen Yanxiu , Sun Yanping , Zhang Dongmei , Qu Lixing , Shang Wei , Wang Jianguo , Liu Xuewu 
Published: February 2023

Abstract

Background:
Given that seizures may be triggered by vaccination, this study aimed to evaluate the risk and correlative factors of seizures in patients with epilepsy (PWE) after being vaccinated against coronavirus disease 2019 (COVID-19).

Methods:
This study retrospectively enrolled PWE who were vaccinated against COVID-19 in the epilepsy centers of 11 hospitals in China. We divided the PWE into two groups as follows: (1) patients who developed seizures within 14 days of vaccination were assigned to the SAV (with seizures after vaccination) group; (2) patients who were seizure-free within 14 days of vaccination were assigned to the SFAV (seizure-free after vaccination) group. To identify potential risk factors for seizure reccurence, the binary logistic regression analysis was performed. Besides, 67 PWE who had not been vaccinated were also included for elucidating the effects of vaccination on seizures recurrence, and binary logistic regression analysis was performed to determine whether vaccination would affect the recurrence rate of PWE who had drug reduction or withdrawal.

Results:
The study included a total of 407 patients; of which, 48 (11.8%) developed seizures within 14 days after vaccination (SAV group), whereas 359 (88.2%) remained seizure-free (SFAV group). The binary logistic regression analysis revealed that duration of seizure freedom (P < 0.001) and withdrawal from anti-seizure medications (ASMs) or reduction in their dosage during the peri-vaccination period were significantly associated with the recurrence of seizures (odds ratio = 7.384, 95% confidence interval = 1.732–31.488, P = 0.007). In addition, 32 of 33 patients (97.0%) who were seizure-free three months before vaccination and had a normal electroencephalogram before vaccination did not have any seizures within 14 days of vaccination. A total of 92 (22.6%) patients experienced non-epileptic adverse reactions after vaccination. Binary logistic regression analysis results showed that vaccine did not significantly affect the recurrence rate of PWE who had the behavior of AMS dose reduction or withdrawal (P = 0.143).

Conclusions:
PWE urgently need protection from the COVID-19 vaccine. PWE who are seizure-free for >3 months before vaccination should be vaccinated. Whether the remaining PWE should be vaccinated depends on the local prevalence of COVID-19. Finally, PWE should be prohibited from discontinuing ASMs or reducing their dosage during the peri-vaccination period.
Surgical treatment of cardiac lipoma: 20 years’ experience in a single center
Authors: Li Linlin , Meng Jian , Zhou Xingtong , Liu Chang , Guo Hongwei , Fan Hongguang 
Published: February 2023

Abstract

Background:
Primary cardiac lipoma is very rare, and no consensus has been developed regarding its ideal treatment strategy. This study reviewed the surgical treatment of cardiac lipomas in 20 patients over 20 years.

Methods:
Twenty patients with cardiac lipomas were treated at Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College from January 1, 2002, to January 1, 2022. The patients’ clinical data and pathological reports were retrospectively analyzed, and the follow-up with a range of 1 to 20 years was conducted.

Results:
The cardiac lipomas were located in the right atrium (RA) or superior vena cava (SVC) in seven patients (35%) (atrium in six patiets and SVC in one patient), left ventricle in eight patients (40%) (left ventricular chamber in four patients and left ventricular subepicardium and myocardium in four patients), right ventricle in three patients (15%) (right ventricular chamber in one patient and right ventricular subepicardial layer and myocardium in two patients), subepicardial interventricular groove in one patient (5%), and pericardium in one patient (5%). Complete resection was achieved in 14 patients (70%), including seven patients with lipomas in the RA or SVC. Incomplete resection occurred in six patients (30%) with lipomas in the ventricles. No perioperative deaths occurred. Long-term follow-up was conducted for 19 patients (95%), including two (10%) who died. Both patients who died had lipomas incompletely resected due to ventricles involvement, and preoperative malignant arrhythmias persisted post-operatively.

Conclusions:
The complete resection rate was high, and the long-term prognosis was satisfactory in patients with cardiac lipomas that did not involve the ventricle. The complete resection rate was low in patients with cardiac lipomas in ventricles; and complications, including malignant arrhythmia, were common. Failure of complete resection and post-operative ventricular arrhythmia are correlated with post-operative mortality.
Safety and Efficacy of Extended Postdischarge Venous Thromboembolism Prophylaxis in Microsurgical Breast Reconstruction
Authors: Hansdorfer Marek A. , Sadowsky Rachel L. , Horen Sydney R. , Becerra Adan Z. , Shenaq Deana S. , Kokosis George 
Published: February 2023

Background:
Discharging patients on extended postoperative venous thromboembolism (VTE) prophylaxis is trending in microsurgical breast reconstruction (MBR). This study investigated contemporary bleeding and thromboembolic complications after MBR and reported postdischarge enoxaparin outcomes.

Methods:
The PearlDiver database was queried for MBR patients who did not receive postdischarge VTE prophylaxis (cohort 1) and MBR patients discharged with enoxaparin for at least 14 days (cohort 2), then queried for hematoma, deep venous thrombosis (DVT), and/or pulmonary embolism. Concurrently, a systematic review was undertaken to identify studies investigating VTE with postoperative chemoprophylaxis.

Results:
In total, 13,541 patients in cohort 1 and 786 patients in cohort 2 were identified. The incidence of hematoma, DVT, and pulmonary embolism were 3.51%, 1.01%, 0.55% in cohort 1, and 3.31%, 2.93%, and 1.78% in cohort 2, respectively. There was no significant difference in hematoma between these two cohorts (P = 0.767); however, a significantly lower rate of DVT (P < 0.001) and pulmonary embolism (P < 0.001) occurred in cohort 1. Ten studies met systematic review inclusion. Only three studies reported significantly lower VTE rates with postoperative chemoprophylaxis. Seven studies found no difference in bleeding risk.

Conclusions:
This is the first study utilizing a national database and a systematic review to investigate extended postoperative enoxaparin in MBR. Overall, rates of DVT/PE seem to be declining compared with previous literature. The results of this study suggest that there remains a lack of evidence supporting extended postoperative chemoprophylaxis, although the therapy appears safe in that it does not increase bleeding risk.
A Sublatissimus Dorsi Abscess Due to Pasteurella multocida Caused by a Cat Scratch
Authors: Ando Junji , Miyata Riyo , Harada Masayuki , Yurugi Satoshi , Imai Yuichiro , Kasahara Kei , Kuwahara Masamitsu 
Published: February 2023

Summary:
We encountered a 51-year-old male patient who was not immunocompromised. Thirteen days before his admission, his right forearm was scratched by his pet cat. Swelling, redness, and purulent discharge appeared at the site, but he did not seek medical attention. He developed a high fever and was hospitalized with a diagnosis of septic shock, respiratory failure, and cellulitis on plain computed tomography. After admission, the swelling on his forearm was relieved with empirical antibiotics, but the symptoms spread from his right axilla to his waist. We suspected necrotizing soft tissue infection and made a trial incision in the lateral chest up to the latissimus dorsi, but were unable to prove it. However, an abscess was later found under the muscle layer. Second incisions were made to allow the abscess to drain. The abscess was relatively serous, and no tissue necrosis was observed. The patient’s symptoms improved rapidly. In retrospect, the patient probably already had the axillary abscess on admission. It may have been detected at this point if contrast-enhanced computed tomography had been performed, and early axillary drainage may have accelerated the patient’s recovery, which could also have prevented the formation of the latissimus dorsi muscle abscess. In conclusion, the Pasteurella multocida infection on the patient’s forearm induced a very unusual presentation and caused an abscess to form under the muscle, unlike necrotizing soft tissue infections. Early contrast-enhanced computed tomography may aid earlier and more appropriate diagnosis and treatment in such cases.
Resolution of hepatic fibrosis after ZFN-mediated gene editing in the PiZ mouse model of human α1-antitrypsin deficiency
Authors: Li Yanfeng , Guha Chandan , Asp Patrik , Wang Xia , Tchaikovskya Tatyana L. , Kim Kenneth , Mendel Matthew , Cost Gregory J. , Perlmutter David H. , Roy-Chowdhury Namita , Fox Ira J. , Conway Anthony , Roy-Chowdhury Jayanta 
Published: February 2023

Background:
α1-antitrypsin deficiency is most commonly caused by a mutation in exon-7 of SERPINA1 (SA1-ATZ), resulting in hepatocellular accumulation of a misfolded variant (ATZ). Human SA1-ATZ-transgenic (PiZ) mice exhibit hepatocellular ATZ accumulation and liver fibrosis. We hypothesized that disrupting the SA1-ATZ transgene in PiZ mice by in vivo genome editing would confer a proliferative advantage to the genome-edited hepatocytes, enabling them to repopulate the liver.

Methods:
To create a targeted DNA break in exon-7 of the SA1-ATZ transgene, we generated 2 recombinant adeno-associated viruses (rAAV) expressing a zinc-finger nuclease pair (rAAV-ZFN), and another rAAV for gene correction by targeted insertion (rAAV-TI). PiZ mice were injected i.v. with rAAV-TI alone or the rAAV-ZFNs at a low (7.5×1010vg/mouse, LD) or a high dose (1.5×1011vg/mouse, HD), with or without rAAV-TI. Two weeks and 6 months after treatment, livers were harvested for molecular, histological, and biochemical analyses.

Results:
Two weeks after treatment, deep sequencing of the hepatic SA1-ATZ transgene pool showed 6%±3% or 15%±4% nonhomologous end joining in mice receiving LD or HD rAAV-ZFN, respectively, which increased to 36%±12% and 36%±12%, respectively, 6 months after treatment. Two weeks postinjection of rAAV-TI with LD or HD of rAAV-ZFN, repair by targeted insertion occurred in 0.10%±0.09% and 0.25%±0.14% of SA1-ATZ transgenes, respectively, which increased to 5.2%±5.0% and 33%±13%, respectively, 6 months after treatment. Six months after rAAV-ZFN administration, there was a marked clearance of ATZ globules from hepatocytes, and resolution of liver fibrosis, along with reduction of hepatic TAZ/WWTR1, hedgehog ligands, Gli2, a TIMP, and collagen content.

Conclusions:
ZFN-mediated SA1-ATZ transgene disruption provides a proliferative advantage to ATZ-depleted hepatocytes, enabling them to repopulate the liver and reverse hepatic fibrosis.
Validation of the PCL-5, PHQ-9, and GAD-7 in a Sample of First Responders
Authors: Ahmadi Aazi , Galusha Jeanine M. , Ponder Warren N. , Carbajal Jose , Schuman Donna L. , Whitworth James , Yockey R. Andrew 
Published: February 2023

Objective
First responders (FRs) commonly present for treatment with complex symptom constellations that frequently include posttraumatic stress disorder, depression, and generalized anxiety. To date, no FR study has validated these measures on a treatment-seeking sample.

Methods
Confirmatory factor analysis was utilized to validate measures that assess these constructs (Posttraumatic Stress Disorder Checklist for DSM-5, Patient Health Questionnaire-9, and Generalized Anxiety Disorder-7) on a treatment-seeking sample of FRs (N = 390).

Results
The seven-factor posttraumatic stress disorder hybrid configuration was the best fit. The best fitting factor model of the depression measure was a two-factor structure, cognitive-affective and somatic depression. Lastly, generalized anxiety was a unidimensional construct.

Limitations
Follow-up studies should validate these measures on each FR subtype.

Conclusion
We position these findings within the FR literature, review clinical implications for providers, and offer recommendations for future research.
Trauma After Cochlear Implantation: The Accuracy of Micro–Computed Tomography and Cone-Beam Fusion Computed Tomography Compared With Histology in Human Temporal Bones
Authors: Iso-Mustajärvi Matti , Silvast Tuomo , Heikka Tuomas , Tervaniemi Jyrki , Calixto Roger , Linder Pia H. , Dietz Aarno 
Published: February 2023

Hypothesis
Micro-computed tomography (micro-CT) and cone-beam computed tomography (CBCT), in conjunction with the image fusion technique, may provide similar results for trauma assessment after cochlear implantation, with respect to the trauma evaluation in preclinical cochlear implant (CI) studies, as the histology.

Background
Before clinical use, novel cochlear implant (CI) designs are tested in temporal bone (TB) studies for usability and risk evaluation. The criterion standard for evaluating intracochlear insertion trauma and electrode location has historically been with histological samples. Progress of modern imaging technology has created alternatives to classic histology. This study compares the micro-CT and CBCT fusion images between histological samples in a preclinical CI study.

Methods
Fourteen freshly frozen TBs were inserted with a lateral wall research CI electrode. All TBs were scanned with CBCT preoperatively and postoperatively. After insertion, the TBs were prepared for micro-CT and histology. Twelve TBs underwent first a micro-CT and then the histological process. The CBCTs were used for image fusion, and all three different methods were used for intracochlear trauma evaluation. The results were compared between methods.

Results
There were 4 of 14 translocations detected with the fusion image method and 3 of 12 with the micro-CT and histology. When compared, the trauma grades converged and were not statistically significant.

Conclusion
The trauma grading based on micro-CT is comparable to the histology. The image fusion technique based on CBCT is less accurate because it relies on an empirical assumption of the basal membrane localization, but it is clinically applicable.
Techniques in Facial Fat Grafting: Optimal Results Based on the Science of Facial Aging
Authors: Rodriguez-Unda Nelson A. , Novak Matthew D. , Rohrich Rod J. 
Published: February 2023

SUMMARY:
A thorough understanding of the science of facial aging is imperative to the precise and natural restoration of a youthful appearance and a hallmark of the aging process is fat atrophy. For this reason, fat grafting has become a keystone of the modern facelift. As a result, fat grafting techniques have been refined to achieve optimal results. This is done through the differential use of fractionated and unfractionated fat throughout the face. The following article reviews a single surgeon’s technique for achieving optimal results in facial fat grafting.
The Application of Double-Layer-Vacuum-Assisted Closure Therapy With Flap Repair of Soft Tissue Defects Near the Eyes or EarDL-VAC Therapy
Authors: Tao Zihan , Xu Jianguo , Dai Haiying , Wu Minliang , Liu Lei , Sun Jie , Xue Chunyu , Wang Yuchong 
Published: February 2023

Objective:
We aimed to introduce and evaluate the safety of double-layer-vacuum-assisted closure (DL-VAC) therapy with flap repair of the wound near the eyes or ears.

Methods:
This study is case reports of 2 patients who underwent DL-VAC therapy for tissue defects near the eyes or ears. The detailed process of the DL-VAC therapy is carefully described in this study. The postoperative wound healing process was closely observed. The biggest concern was the treatment outcome of DL-VAC therapy on the eyes and ears.

Results:
Two patients underwent DL-VAC therapy due to their soft tissue defects close to the eyes or ears. Both patients achieved primary wound healing and the flaps survived during the follow‐up period, which ranged from 1 to 24 months. Patients did not receive any dressing change until the VAC device was removed on the 5th to 7th postoperative day. No severe complications appeared and the patients were satisfied with both appearance and function.

Conclusions:
Double-layer-vacuum-assisted closure therapy is an effective and safe option for the treatment of wounds near the eyes and ears.
Circulating Tumor DNA as a Prognostic Factor in Patients With Resectable Hepatic Metastases of Uveal Melanoma
Authors: Mariani Pascale , Bidard François-Clément , Rampanou Aurore , Houy Alexandre , Servois Vincent , Ramtohul Toulsie , Pierron Gaelle , Chevrier Marion , Renouf Benjamin , Lantz Olivier , Gardrat Sophie , Vincent-Salomon Anne , Roman-Roman Sergio , Rodrigues Manuel , Piperno-Neumann Sophie , Cassoux Nathalie , Stern Marc-Henri , Renault Shufang 
Published: February 2023

Objective:
We report here the results of a prospective study of circulating tumor DNA (ctDNA) detection in patients undergoing uveal melanoma (UM) liver metastases resection (NCT02849145).

Background:
In UM patients, the liver is the most common and often only site of metastases. Local treatments of liver metastases, such as surgical resection, have a likely benefit in selected patients.

Methods:
Upon enrollment, metastatic UM patients eligible for curative liver surgery had plasma samples collected before and after surgery. GNAQ/GNA11 mutations were identified in archived tumor tissue and used to quantify ctDNA by droplet digital polymerase chain reaction which was then associated with the patient’s surgical outcomes.

Results:
Forty-seven patients were included. Liver surgery was associated with a major increase of cell-free circulating DNA levels, with a peak 2 days after surgery (∼20-fold). Among 40 evaluable patients, 14 (35%) had detectable ctDNA before surgery, with a median allelic frequency of 1.1%. These patients experienced statistically shorter relapse-free survival (RFS) versus patients with no detectable ctDNA before surgery (median RFS: 5.5 vs 12.2 months; hazard ratio=2.23, 95% CI: 1.06–4.69, P=0.04), and had a numerically shorter overall survival (OS) (median OS: 27.0 vs 42.3 months). ctDNA positivity at postsurgery time points was also associated with RFS and OS.

Conclusions:
This study is the first to report ctDNA detection rate and prognostic impact in UM patients eligible for surgical resection of their liver metastases. If confirmed by further studies in this setting, this noninvasive biomarker could inform treatment decisions in UM patients with liver metastases.
Non-reciprocity induces resonances in a two-field Cahn–Hilliard model
Authors: Frohoff-Hülsmann Tobias , Thiele Uwe , Pismen Len M. 
Published: February 2023

We consider a non-reciprocally coupled two-field Cahn–Hilliard system that has been shown to allow for oscillatory behaviour and suppression of coarsening. After introducing the model, we first review the linear stability of steady uniform states and show that all instability thresholds are identical to the ones for a corresponding two-species reaction–diffusion system. Next, we consider a specific interaction of linear modes—a ‘Hopf–Turing’ resonance—and derive the corresponding amplitude equations using a weakly nonlinear approach. We discuss the weakly nonlinear results and finally compare them with fully nonlinear simulations for a specific conserved amended FitzHugh–Nagumo system. We conclude with a discussion of the limitations of the employed weakly nonlinear approach.This article is part of the theme issue ‘New trends in pattern formation and nonlinear dynamics of extended systems’.
Antimicrobial Resistance Potential of Microbes Isolated from Fish Ponds in Ikorodu, Lagos State, Nigeria.
Authors: Aina Oladunni Rita , Olaleye Oluremi Nurudee 
Published: February 2023

ABSTRACT
Aquacultures are practised in different water confinements which include plastic ponds, concrete and earthen and are known to harbour pathogens. In aquaculture (fish rearing) the use of antibiotics is on the increase where they are used as disease eradicators and as growth promoters. This study sought to investigate the antibiotic resistance potential of bacteria isolated from fish ponds in Ikorodu, Lagos State. A total number of ten water samples were collected from five different sampling points at depth 1.5m within the ponds, close to the outlets and at the outlets from the cat-fish and tilapia-fish ponds. These were serially diluted and inoculated and the pure isolates were subjected to antibiotics sensitivity testing using Kirby- Bauer’s disc diffusion and minimum inhibitory concentration methods. Pure isolates which were found to show multiple resistances to antibiotics were characterized based on their morphology and biochemical characteristics. The isolates were further identified by molecular analysis using 16SrRNA gene detection and sequencing. The Antibiotic susceptibility test showed that the isolates were resistant to ceftazidime, cefuroxime, nitrofurantoin, ampicillin, amoxycillin, clavulanate. gentamicin, ciprofloxacin and all the isolates were susceptible to Ofloxacin. The molecular analysis revealed that the organisms which showed multiple resistances to antibiotics were Azotobacter chroococcum, Escherichia Coli, Proteus mirabilis and Pseudomonas aeruginosa. In conclusion this study has revealed the need for good management of aquaculture facilities in order to avoid zoonotic diseases; also monitoring of antibiotics usage in fish ponds should be given high priority to avoid resistant genes from being transferred to other bacteria of human clinical significance.