bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trait variations for seed physical characteristics in chickpea (Cicer arietinum L.) from the Western Himalayas
Authors: Fayaz Humara , Wani Aijaz A. , Mir Asma Hamid , Khan Raheel Shafeeq , Jan Farkhanda , Yasin Mohammad , Bhat Mohd. Ashraf , Mir Reyazul Rouf 
Published: February 2023

AbstractChickpea (Cicer arietinum L.) is one of the most important grain legumes after common bean. In the present study, a total of 35 chickpea genotypes including desi and Kabuli types of cultivated chickpea (C. arietinum) were evaluated for seed physical characteristics that are very important for seed storage, processing and determining seed quality traits. Therefore, it has become imperative to study the seed physical characteristics of chickpea during the present study. The analysis of data revealed that substantial variation is present among the genotypes under study with respect to seed physical characteristics such as seed size, seed volume, seed density, hydration capacity, hydration index, swelling capacity and swelling index. These traits are considered important to determine the seed rate at the time of sowing of the crop, cooking quality, storage and processing and thereby have a direct bearing on the economics of chickpea consumption. Our results indicated that the desi, Kabuli and pea-shaped genotypes differ significantly in seed physical characteristics with overall superior trait performance of the Kabuli type compared to other seed types, making them superior with respect to consumer/market demands.
RNO-G detection perspectives of binary neutron star mergers
Authors: Cataldo Maddalena ,   for the RNO-G Collaboration
Published: February 2023

AbstractThe Radio Neutrino Observatory Greenland (RNO-G) is currently being deployed and it is currently gathering data. As a precursor and complementary detector to the future radio array of IceCube-Gen2 in Antarctica, it will explore mainly the Northern sky via in-ice radio detection technique. The total array configuration includes 35 radio stations and will be fully completed within three years from now. The antennas will register the radio signals produced by the Askaryan effect in cascades generated in ice by neutrinos. RNO-G’s scientific purpose is to detect UHE neutrinos at energies above 10 PeV. Due to the attenuation length of radio waves in ice (order of 1 km) the radio detection allows to address neutrino energies above several PeV. The detector will reach unprecedented sensitivity in the scale from tens of PeV up to EeV. Models predict GRBs induced by binary neutron star mergers as likely transient sources of such highly energetic neutrinos. The current study of NS-NS mergers will therefore possibly be complemented by future RNO-G detections through multimessenger temporal and spatial coincidence, including an alert system. In this presentation, we will describe the instrument capabilities and explore the possibility of detection of such sources with RNO-G.
‘It’s been quite a poor show’ – exploring whether practitioners working for Improving Access to Psychological Therapies (IAPT) services are culturally competent to deal with the needs of Black, Asian and Minority Ethnic (BAME) communities
Authors: Faheem Afsana 
Published: February 2023

Abstract
Cultural competency is a core clinical skill. Yet, psychological therapists may be inadequately trained to deal with the needs of service users from Black, Asian and Minority Ethnic (BAME) backgrounds. This can lead to dissatisfaction with mental health services, disengagement from therapy, and poorer treatment outcomes when compared to the White British population. The aim of this study was to explore whether practitioners working for Improving Access to Psychological Therapies (IAPT) services are culturally competent to deal with the needs of diverse communities. Semi-structured interviews were carried out with a range of practitioners, from early career psychological wellbeing practitioners (PWPs) to senior cognitive behavioural therapists (n=16). Reflexive thematic analysis (RTA) was used to analyse the data, guided by a six-phase process to produce a robust pattern-based analysis. Overall, three themes were generated: (1) encountering cultural dissonance within therapy; (2) challenges in making cultural adaptations to therapy; and (3) identifying cultural competency needs. Out of sixteen participants, only nine therapists received one-day formal training throughout their therapeutic career, whilst seven reported receiving no cultural competence training at all. Overall, it appears that there is an urgent need and desire for therapists to be offered cultural competency training so that they can better serve BAME communities. Clinical implications and future recommendations are made.

Key learning aims

(1)
To briefly introduce cross-cultural theoretical models that may assist mental health professionals to think critically about Western notions of therapy and whether they are suited to the needs of ethnic minority communities.

(2)
To consider challenges IAPT practitioners encounter during therapy and identify examples of good practice.

(3)
To explore to what extent IAPT practitioners feel culturally competent to deal with the needs of BAME communities.

(4)
To encourage IAPT services and decision makers (e.g. training bodies and commissioners) to enhance cultural competence training so that practitioners can better serve ethnic minority communities.
Tolerance of muscadine grape to 2,4-D choline postemergence-directed
Authors: Sims Kira C. , Mitchem Wayne E. , Jennings Katherine M. , Monks David W. , Jordan David L. , Hoffmann Mark 
Published: February 2023

AbstractField studies were conducted in commercial muscadine vineyards in western North Carolina in 2018 and eastern North Carolina in 2019, 2020, and 2021 to determine tolerance of younger (< 9 yr) and older (≥ 9 yr) bearing muscadine grapevines to 2,4-D directed beneath the crop postemergence (POST). Treatments included 2,4-D choline at 0, 0.53, 1.06, 1.60, and 2.13 kg ae ha−1 applied as a single treatment in May or June (spring) at immediate pre-bloom, and sequential treatments at 0.53 followed by (fb) 0.53, 1.06 fb 1.06, 1.6 fb 1.6, or 2.13 fb 2.13 kg ha−1. The first sequential treatment was applied in spring fb another application of the same amount in July (summer) at pre-veraison. No differences in injury on muscadine grapevines were observed from 2,4-D treatments. Differences among treatments were not observed for yield of younger vines. However, for older vines, a difference due to 2,4-D rate was observed in 2018, when yield was higher when 2,4-D was applied at 1.6 kg ha−1 compared with nontreated grapevines, and when 2,4-D was applied at 0.53 and 2.13 kg ha−1. A rate-by-timing interaction was observed in 2019 when yield was lower from 0.53 kg ha−1 2,4-D summer application compared with all other summer treatments but similar to the nontreated. However, no biological pattern was observed from either of these differences. No differences among treatments were observed for fruit pH, titratable acidity, or soluble solid content of either younger or older vines.
Transplant broccoli and collard response to the residual activity of glyphosate applied preplant
Authors: Wright-Smith Hannah E. , Culpepper A. Stanley , Randell-Singleton Taylor M. , Vance Jenna C. 
Published: February 2023

AbstractCole crops including broccoli and collard contribute more than $119 million to Georgia’s farm gate value yearly. To ensure maximum profitability, these crops must be planted into weed-free fields. Glyphosate is a tool often used to help achieve this goal because of its broad-spectrum activity on weeds coupled with the knowledge that it poses no threat to the succeeding crop when used as directed. However, recent research suggests that with certain soil textures and production systems, the residual soil activity of glyphosate may damage some crops. Therefore, field experiments were conducted in fall 2019 and 2020 to evaluate transplanted broccoli and collard response to glyphosate applied preplant onto bare soil and what practical mitigation measures could be implemented to reduce crop injury. Herbicide treatments consisted oGf 0, 2.5, or 5 kg ae ha−1 glyphosate applied preplant followed by 1) no mitigation measure, 2) tillage, 3) irrigation, or 4) tillage and irrigation prior to transplanting broccoli and collard by hand. When no mitigation was implemented, the residual activity of glyphosate at 2.5 and 5.0 kg ae ha−1 resulted in 43% to 71% and 79% to 93% injury to broccoli and collard transplants, respectively. This resulted in a 35% to 50% reduction in broccoli marketable head weights and 63% to 71% reduction in collard leaf weights. Irrigation reduced visible damage by 28% to 48%, whereas tillage reduced injury by 43% to 76%, for both crops. Irrigation alleviated yield losses for broccoli but only tillage eliminated yield loss for both crops. Care must be taken when transplanting broccoli and collard into a field recently treated with glyphosate at rates ≥2.5 kg ae ha−1. Its residual activity can damage transplants with injury levels influenced by glyphosate rate, and tillage or irrigation after application and prior to planting.
Multiproxy Reconstructions of Integral Energy Spectra for Extreme Solar Particle Events of 7176 BCE, 660 BCE, 775 CE, and 994 CE
Authors: Koldobskiy Sergey , Mekhaldi Florian , Kovaltsov Gennady , Usoskin Ilya 
Published: February 2023

AbstractExtreme solar particle events (ESPEs) are rare and the most potent known processes of solar eruptive activity. During ESPEs, a vast amount of cosmogenic isotopes (CIs) 10Be, 36Cl, and 14C can be produced in the Earth's atmosphere and deposited in natural stratified archives. Accordingly, CI measurements in these archives allow us to evaluate particle fluxes during ESPEs. In this work, we present a new method of ESPE fluence (integral flux) reconstruction based on state‐of‐the‐art modeling advances, allowing to fit together different CI data within one model. We represent the ESPE fluence as an ensemble of scaled fluence reconstructions for ground‐level enhancement (GLE) events registered by the neutron monitor network since 1956 coupled with satellite and ionospheric measurements data. Reconstructed ESPE fluences appear softer in its spectral shape than earlier estimates, leading to significantly higher estimates of the low‐energy (E < 100 MeV) fluence. This makes ESPEs even more dangerous for modern technological systems than previously believed. Reconstructed ESPE fluences are fitted with a modified Band function, which eases the use of obtained results in different applications.
Salmonella Typhimurium expressing chromosomally integrated Schistosoma mansoni Cathepsin B protects against schistosomiasis in mice
Authors: Hassan Adam S. , Houle Sébastien , Labrie Lydia , Perera Dilhan J. , Dozois Charles M. , Ward Brian J. , Ndao Momar 
Published: February 2023

AbstractSchistosomiasis threatens hundreds of millions of people worldwide. The larval stage of Schistosoma mansoni migrates through the lung and adult worms reside adjacent to the colonic mucosa. Several candidate vaccines are in preclinical development, but none is designed to elicit both systemic and mucosal responses. We have repurposed an attenuated Salmonella enterica Typhimurium strain (YS1646) to express Cathepsin B (CatB), a digestive enzyme important for the juvenile and adult stages of the S. mansoni life cycle. Previous studies have demonstrated the prophylactic and therapeutic efficacy of our plasmid-based vaccine. Here, we have generated chromosomally integrated (CI) YS1646 strains that express CatB to produce a viable candidate vaccine for eventual human use (stability, no antibiotic resistance). 6–8-week-old C57BL/6 mice were vaccinated in a multimodal oral (PO) and intramuscular (IM) regimen, and then sacrificed 3 weeks later. The PO + IM group had significantly higher anti-CatB IgG titers with greater avidity and mounted significant intestinal anti-CatB IgA responses compared to PBS control mice (all P < 0.0001). Multimodal vaccination generated balanced TH1/TH2 humoral and cellular immune responses. Production of IFNγ by both CD4+ and CD8+ T cells was confirmed by flow cytometry (P < 0.0001 & P < 0.01). Multimodal vaccination reduced worm burden by 80.4%, hepatic egg counts by 75.2%, and intestinal egg burden by 78.4% (all P < 0.0001). A stable and safe vaccine that has both prophylactic and therapeutic activity would be ideal for use in conjunction with praziquantel mass treatment campaigns.
A neurodevelopmental epigenetic programme mediated by SMARCD3–DAB1–Reelin signalling is hijacked to promote medulloblastoma metastasis
Authors: Zou Han , Poore Bradley , Brown Emily E. , Qian Jieqi , Xie Bin , Asimakidou Evridiki , Razskazovskiy Vladislav , Ayrapetian Deanna , Sharma Vaibhav , Xia Shunjin , Liu Fei , Chen Apeng , Guan Yongchang , Li Zhengwei , Wanggou Siyi , Saulnier Olivier , Ly Michelle , Fellows-Mayle Wendy , Xi Guifa , Tomita Tadanori , Resnick Adam C. , Mack Stephen C. , Raabe Eric H. , Eberhart Charles G. , Sun Dandan , Stronach Beth E. , Agnihotri Sameer , Kohanbash Gary , Lu Songjian , Herrup Karl , Rich Jeremy N. , Gittes George K. , Broniscer Alberto , Hu Zhongliang , Li Xuejun , Pollack Ian F. , Friedlander Robert M. , Hainer Sarah J. , Taylor Michael D. , Hu Baoli 
Published: February 2023

AbstractHow abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly available datasets with our newly generated data reveal that SMARCD3 (also known as BAF60C) regulates Disabled 1 (DAB1)-mediated Reelin signalling in Purkinje cell migration and MB metastasis by orchestrating cis-regulatory elements at the DAB1 locus. We further identify that a core set of transcription factors, enhancer of zeste homologue 2 (EZH2) and nuclear factor I X (NFIX), coordinates with the cis-regulatory elements at the SMARCD3 locus to form a chromatin hub to control SMARCD3 expression in the developing cerebellum and in metastatic MB. Increased SMARCD3 expression activates Reelin–DAB1-mediated Src kinase signalling, which results in a MB response to Src inhibition. These data deepen our understanding of how neurodevelopmental programming influences disease progression and provide a potential therapeutic option for patients with MB.
Russian nuclear energy diplomacy and its implications for energy security in the context of the war in Ukraine
Authors: Szulecki Kacper , Overland Indra 
Published: February 2023

AbstractSince Russia invaded Ukraine in February 2022, the possibility of reducing Europe’s energy dependence on Russian resources has been hotly debated. The fossil fuel industries received most attention as European Union leaders first introduced gradual sanctions on Russian coal and later on oil and gas, while Russia responded with supply cuts. However, Russia’s role as a major player in the global nuclear power sector has remained largely below the sanctions radar, despite dependencies on Russian nuclear technology, uranium supplies and handling of spent nuclear fuel. Here we analyse the state nuclear company Rosatom and its subsidiaries as tools of Russian energy statecraft. We map the company’s global portfolio, then categorize countries where Russia is active according to the degree and intensity of dependence. We offer a taxonomy of long-term energy dependencies, highlighting specific security risks associated with each of them. We conclude that the war and Russia’s actions in the energy sector will undermine Rosatom’s position in Europe and damage its reputation as a reliable supplier, but its global standing may remain strong.
Development of controlled nanosphere lithography technology
Authors: Osipov Artem A. , Gagaeva Alina E. , Speshilova Anastasiya B. , Endiiarova Ekaterina V. , Bespalova Polina G. , Osipov Armenak A. , Belyanov Ilya A. , Tyurikov Kirill S. , Tyurikova Irina A. , Alexandrov Sergey E. 
Published: February 2023

AbstractThis work is devoted to the development of nanosphere lithography (NSL) technology, which is a low-cost and efficient method to form nanostructures for nanoelectronics, as well as optoelectronic, plasmonic and photovoltaic applications. Creating a nanosphere mask by spin-coating is a promising, but not sufficiently studied method, requiring a large experimental base for different sizes of nanospheres. So, in this work, we investigated the influence of the technological parameters of NSL by spin-coating on the substrate coverage area by a monolayer of nanospheres with a diameter of 300 nm. It was found that the coverage area increases with decreasing spin speed and time, isopropyl and propylene glycol content, and with increasing the content of nanospheres in solution. Moreover, the process of controllably reducing the size of nanospheres in inductively coupled oxygen plasma was studied in detail. It was determined that increasing the oxygen flow rate from 9 to 15 sccm does not change the polystyrene etching rate, whereas changing the high-frequency power from 250 to 500 W increases the etching rate and allows us to control the decreasing diameter with high accuracy. Based on the experimental data, the optimal technological parameters of NSL were selected and the nanosphere mask on Si substrate was created with coverage area of 97.8% and process reproducibility of 98.6%. Subsequently reducing the nanosphere diameter lets us obtain nanoneedles of various sizes, which can be used in field emission cathodes. In this work, the reduction of nanosphere size, silicon etching, and removal of polystyrene residues occurred in unified continuous process of plasma etching without sample unloading to atmosphere.
New evidence on the rural poverty and energy choice relationship
Authors: Di Falco Salvatore , Lynam Gary 
Published: February 2023

AbstractWe combine a global micro level dataset that includes 17 different rural Sub-Saharan countries with satellite information about precipitation during the growing season to estimate the impact of economic conditions on energy choice. Differently from the existing literature, we aim to causally estimate the impact of household welfare variation on the likelihood of choosing a specific energy source. It is found, consistent with theory, that increases in income do determine an increase in the likelihood of using relatively cleaner and more efficient sources of fuel. We find, however, that this impact is quantitatively very small. Results hold conditional on assets, wealth and a large battery of controls and fixed effects. Policy implications are developed.
Nine patients with KCNQ2-related neonatal seizures and functional studies of two missense variants
Authors: Chokvithaya Suphalak , Caengprasath Natarin , Buasong Aayalida , Jantasuwan Supavadee , Santawong Kanokwan , Leela-adisorn Netchanok , Tongkobpetch Siraprapa , Ittiwut Chupong , Saengow Vitchayaporn Emarach , Kamolvisit Wuttichart , Boonsimma Ponghatai , Bongsebandhu-phubhakdi Saknan , Shotelersuk Vorasuk 
Published: February 2023

AbstractMutations in KCNQ2 encoding for voltage-gated K channel subunits underlying the neuronal M-current have been associated with infantile-onset epileptic disorders. The clinical spectrum ranges from self-limited neonatal seizures to epileptic encephalopathy and delayed development. Mutations in KCNQ2 could be either gain- or loss-of-function which require different therapeutic approaches. To better understand genotype–phenotype correlation, more reports of patients and their mutations with elucidated molecular mechanism are needed. We studied 104 patients with infantile-onset pharmacoresistant epilepsy who underwent exome or genome sequencing. Nine patients with neonatal-onset seizures from unrelated families were found to harbor pathogenic or likely pathogenic variants in the KCNQ2 gene. The p.(N258K) was recently reported, and p. (G279D) has never been previously reported. Functional effect of p.(N258K) and p.(G279D) has never been previously studied. The cellular localization study demonstrated that the surface membrane expression of Kv7.2 carrying either variant was decreased. Whole-cell patch-clamp analyses revealed that both variants significantly impaired Kv7.2 M-current amplitude and density, conductance depolarizing shift in voltage dependence of activation, membrane resistance, and membrane time constant (Tau), indicating a loss-of-function in both the homotetrameric and heterotetrameric with Kv7.3 channels. In addition, both variants exerted dominant-negative effects in heterotetrameric with Kv7.3 channels. This study expands the mutational spectrum of KCNQ2- related epilepsy and their functional consequences provide insights into their pathomechanism.
Sodium benzoate attenuates 2,8-dihydroxyadenine nephropathy by inhibiting monocyte/macrophage TNF-α expression
Authors: Oshima Yoichi , Wakino Shu , Kanda Takeshi , Tajima Takaya , Itoh Tomoaki , Uchiyama Kiyotaka , Yoshimoto Keiko , Sasabe Jumpei , Yasui Masato , Itoh Hiroshi 
Published: February 2023

AbstractSodium benzoate (SB), a known D-amino acid oxidase (DAO) enzyme inhibitor, has an anti-inflammatory effect, although its role in renal damage has not been explored. 2,8-dihydroxyadenine crystal induced chronic kidney disease, in which TNF-α is involved in the pathogenesis, was established by oral adenine administration in C57BL/6JJcl mice (AdCKD) with or without SB to investigate its renal protective effects. SB significantly attenuated AdCKD by decreasing serum creatinine and urea nitrogen levels, and kidney interstitial fibrosis and tubular atrophy scores. The survival of AdCKD mice improved 2.6-fold by SB administration. SB significantly decreased the number of infiltrating macrophages observed in the positive F4/80 immunohistochemistry area and reduced the expression of macrophage markers and inflammatory genes, including TNF-α, in the kidneys of AdCKD. Human THP-1 cells stimulated with either lipopolysaccharide or TNF-α showed increased expression of inflammatory genes, although this was significantly reduced by SB, confirming the anti-inflammatory effects of SB. SB exhibited renal protective effects in AdCKD in DAO enzyme deficient mice, suggesting that anti-inflammatory effect of SB was independent of DAO enzyme activity. Moreover, binding to motif DNA sequence, protein level, and mRNA level of NF-κB RelB were significantly inhibited by SB in AdCKD kidneys and lipopolysaccharide treated THP-1 cells, respectively. We report that anti-inflammatory property of SB is independent of DAO enzymatic activity and is associated with down regulated NF-κB RelB as well as its downstream inflammatory genes such as TNF-α in AdCKD.