bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ferrostatin-1 facilitated neurological functional rehabilitation of spinal cord injury mice by inhibiting ferroptosis
Authors: Zhou Zhenhai , Luo Hao , Yu Honggui , Liu Zhiming , Zhong Junlong , Xiong Jiachao , Cao Kai 
Published: May 2023

Abstract
Background
To seek the potential therapy for spinal cord injury, Ferrostatin-1, the first ferroptosis inhibitor, was indicated in spinal cord injury mice, to identify the therapeutic effect. (2)Methods: spinal cord injury model was established by a modified Allen’s method. Then, Ferrostatin-1 was administrated by intraspinal injection. Cortical evoked motorpotential, BMS was indicated to assess the neurological function rehabilitation. H&E, Nissl's staining, NeuN, and GFAP immunofluorescence were used to identify the histological manifestation on the injured spinal cord. Spinosin a selective small molecule activator of the Nrf2/HO-1 pathway was administrated to verify the underlying mechanism of Ferrostatin-1. (3) Results: Ferrostatin-1 is conducive to the rehabilitation of cortical evoked motorpotential, BMS scores, synchronized with improvement in the histological manifestation of neuron survival and scar formation and synchronized with improvement in the histological manifestation of neuron survival and scar formation. Spinosin disturbed the benefits of ferrostatin-1 administrating on histological and neurobehavioral manifestation by deranging the Nrf2/HO-1 pathway. (4) Conclusions: ferrostain-1 improved rehabilitation of SCI mice by regulating ferroptosis through the Nrf2/HO-1 signaling pathway.
Contamination Acts as a Genotype-Dependent Barrier to Gene Flow, Causing Genetic Erosion and Fine-Grained Population Subdivision in Mussels from the Strait of Istanbul
Authors: Theodorakis Christopher W. , Meyer Mary-Ann , Okay Oya , Yakan Sevil Deniz , Schramm Karl-Werner 
Published: May 2023

Abstract
This study provides evidence of fine-grained genetic structuring in Mediterranean mussels (Mytilus galloprovencialis) from the Strait of Istanbul, caused by barriers to gene flow via contaminant-mediated selection. In this study, mitochondrial D-loop sequences were analyzed in mussels from 8 localities, all less than 30 kilometers apart, with differing contaminant loads. The results were: 1) Intra-population genetic differentiation (ΦST) between sites with high and low contaminant loads was high (up to 0.459), even at distances of only a few kilometers. 2) Genetic diversity was negatively correlated with the contaminant load (“genetic erosion”). 3) There was evidence of selection, based on haplotype frequencies and neutrality tests (Tajima’s D), with purifying selection at the most contaminated site and balancing selection at the least contaminated. 4) Genetic distance was not correlated with geographic distance (no isolation-by-distance), but was correlated with contaminant load at each site. 5) Population dendrograms and Bayesian estimators of migration indicated that gene flow between sites was affected by contamination. For the dendrograms of the sampling sites, the clades clustered according to contaminant load more than geographic distance. Overall, the conclusions are that 1) contamination serves as a genotype-dependent dispersal barrier (i.e., selection-mediated gene flow) for pelagic mussel larvae, leading strong population differentiation over short distances, and 2) this selection-mediated gene flow also lead to genetic erosion within contaminated sites. These effects may be more pronounced in the Strait of Istanbul than in other locations because of the riverine nature and strong, uni-directional current of the strait.
Interannual Teleconnections in the Sahara Temperatures Associated with the North Atlantic Oscillation (NAO) during Boreal Winter
Authors: Zhou Liming , Hua Wenjian , Nicholson Sharon E , Clark Joseph P 
Published: May 2023

Abstract
The North Atlantic Oscillation (NAO) is the most recognized and leading mode of atmospheric variability observed over the Atlantic sector of the Northern Hemisphere, and its impacts on weather and climate over the North Atlantic and Eurasia via large-scale teleconnections have been extensively studied. Here we use a multidata synthesis approach to analyze surface and tropospheric variables from multiple long-term observational and reanalysis datasets to identify the NAO’s footprint on interannual temperature variability over the vast but least-studied Sahara Desert during December–January–February–March for the satellite era (1979-2022) and much longer periods. Our results present evidence for a solid teleconnection pattern in surface and tropospheric temperatures associated with the NAO over the Sahara and document some major spatial–temporal and vertical characteristics of this pattern. It is found that the Saharan temperature anomalies are negatively correlated with the NAO index and this correlation is very strong, consistent, and statistically significant between different periods and across different datasets. The teleconnection is closely linked to large-scale circulation anomalies throughout the troposphere over the North Atlantic–Sahara sector, where the anomalous horizontal wind components and geopotential height exhibit opposite changes in sign with altitude from the lower to upper troposphere. During the negative NAO− (positive NAO+) phase, above-normal (below-normal) temperatures over the Sahara could be mainly explained by three major processes: (1) advection of climatological warm and moist (cold and dry) air over the North Atlantic (northern higher latitudes) by the anomalous southwesterly (northeasterly) flow in the lower troposphere; (2) advection of anomalous North Atlantic warm (cold) air by the climatological strong westerlies in the middle and upper troposphere; and (3) strengthened (weakened) vertical mixing in the atmospheric boundary layer. These results suggest that the NAO plays an important role in modulating the interannual temperature variability over the Sahara, and that this NAO footprint is mostly realized through horizontal temperature advection and vertical heat transfer by turbulent mixing.
A multi-scale approach to study palm-weevils in a tropical agroecosystem
Authors: Vásquez-Ordóñez Aymer Andrés , Torres-López Wilmar , Monmany-Garzia A. Carolina 
Published: May 2023

Abstract
Context
The recent phytosanitary crisis caused by the stem borer weevils Dynamis borassi and Rhynchophorus palmarum in peach palms (Bactris gasipaes) seriously affected the economy of local families in Colombia. To understand the possible ecological mechanisms favoring the pest, the weevil-palm system needs to be studied from a multi-scale perspective.
Objectives
We evaluated the combined effects of agricultural management, landscape, topography, soil, and climate variables on infestation levels of peach palm caused by the weevils D. borassi and R. palmarumin the Colombian southwest to understand underlying ecological drivers of the current phytosanitary crisis.
Methods
The palm infestation levels (response variable) and local agronomic practices were recorded in 32 sites across Colombia's Pacific, Andes, and Amazon natural regions. For each peach palm crop, landscape variables were estimated from classified Sentinel-2 images, soil variables were extracted from the Soil Grids 250 m database, and climate variables were extracted from the CHIRPS and CHIRTS databases. Explanatory variables were analyzed using Generalized Linear Models and Partial Least Squares-Generalized Linear Models.
Results
Landscape variables, specifically the number and shape of non-forest patches, had a greater influence on peach palm infestation levels than other environmental variables, possibly related to the decrease in the density of Oenocarpus batatua, the natural palm host for D. borassi, leading it to switch to the peach palm.
Conclusions
A multi-scale approach of the weevil-palm interaction enabled us to identify an ecological mechanism that has not been proposed before. Our recommendation for families depending on the palm is to design palm management with a landscape perspective that considers the conservation of alternative hosts for the weevils in agroforestal plots.
Spatiotemporal patterns of rising annual plant abundance in grasslands of the western Pacific Northwest, USA
Authors: Reed Paul B. , Hallett Lauren M. 
Published: May 2023

Abstract
Context:
Plant communities are undergoing compositional changes that affect ecosystem function. Invasions are not always uniform across the landscape due to heterogenous topographic and edaphic conditions. To predict areas most at risk of invasion, it is necessary to identify the landscape drivers affecting plant abundance.
Objectives
Non-native annuals have been increasing across the western USA. Here, we quantified the change in annual plant abundance and identified landscape sensitivities contributing to that change over the past 35 years.
Methods
We focused on Willamette Valley (Oregon) grasslands because they represent a new horizon in this phenomenon. To understand the spatiotemporal patterns of annual plant abundances between 1986–2020, we combined a remote-sensing vegetation cover dataset from the Rangeland Analysis Platform with gridded soils data and topographic variables. We determined the rate of change in percent cover for each pixel and regressed cover against heat load, soil depth, and sand content to determine landscape sensitivity through time for > 5,975 hectares.
Results
We found a tendency toward increasing annual plant cover, with a median gain of + 15% cover among pixels experiencing a significant increase. However, change was uneven across the landscape, with annual plant cover increasing markedly in areas with high heat load and shallower soils.
Conclusions
We identified steep, south-facing slopes as being particularly sensitive to rising annual plant cover. Non-native annual invasions may be lagging in this region compared to what has already occurred in California and the Great Basin, but trends here suggest it may just be a matter of time.
Changes in the South Atlantic Dipole, relations with the Interdecadal Pacific Oscillation and impacts on South America
Authors: Bier Anderson Augusto , Ambrizzi Tércio , Drumond Anita 
Published: May 2023

Abstract
Changes in physical features of the South Atlantic Ocean (SAO) have been observed over the last few decades as a result of anthropogenic climate change. As an example, the increase in Agulhas Leakage, the continental melting of Greenland and changes in wind-forced ocean circulation are factors associated with changes in the Atlantic Meridional Overturning Circulation (AMOC). Within this context, important means of ocean-atmosphere interaction on a climate scale, such as the main mode of coupled variability of the SAO, known as the South Atlantic Dipole (SAD), may also be subject to variations. This work aimed to investigate changes in the SAD over different phases of the Interdecadal Pacific Oscillation (IPO) since the 1950s and assess their impacts on the climate of South America during the austral summer. The main finding is a southward shift of the entire SAD pattern over the analyzed period, consistent with other observed oceanic and atmospheric changes attributed to global warming. Associated with that, a change from a tripole to a dipole pattern of precipitation anomalies was observed over the South American continent during summer. Besides, the SAD displayed an alternate relationship with IPO phases, with a positive (negative) correlation with Pacific Niño SST anomalies during the IPO negative (positive) phase.
The efficacy of Percutaneous transluminal angioplasty and Arteriovenous fistula reconstruction for immature arteriovenous fistula
Authors: Han Yu , Yanqing Chi , Baoxing Wang 
Published: May 2023

Abstract
Background
To access the efficacy of percutaneous transluminal angioplasty and arteriovenous fistula reconstruction for immature arteriovenous fistula, compare the long-term patency and post-operative complications between them.
Materials and Methods
The medical records and Hemodialysis record sheets from 44 patients between May 2020 and January 2022 who underwent percutaneous transluminal angioplasty or arteriovenous fistula reconstruction treatment for immature autogenous arteriovenous fistula (AVF) were retrospectively reviewed. The patients were divided into two groups according to the type of surgery they received, including 25 patients in the PTA group and 19 patients in the AVF reconstruction group. Clinical outcomes were included, such as the primary and secondary patency rates following the procedure, maturation time, peak systolic velocity (PSV) of brachial artery, maximum pump-controlled blood flow at initial dialysis, and post-operative complications rates in the two groups.
Results
Technical and clinical success was achieved in 100% of the 44 cases. For patients who underwent percutaneous transluminal angioplasty, the primary patency rate at 3, 6, and 9 months was 84.0%, 68.0%, 60.0%, and the secondary patency rate was 92.0%, 84.0%, 80.0%, respectively. And for patients who underwent arteriovenous fistula reconstruction, the primary patency rate at 3, 6, and 9 months was 89.5%, 73.7%, 68.4%, and the secondary patency rate was 100.0%, 94.7%, 94.7%, respectively. There were no significant differences between the two groups in terms of patency rates (p > .050). In patients whose maturation was successful, the average maturation time of fistula after the PTA procedure was 19.36 ± 13.94 days, and 58.63 ± 18.95 days for the reconstruction procedure (p < .010). The PSV of brachial artery before and after the procedure was 87.64 ± 23.87 cm/s and 153.20 ± 21.69 cm/s in PTA group, for reconstruction group, the number was 86.26 ± 20.59 cm/s and 151.26 ± 29.94 cm/s, respectively. No statistically significant differences (p > .050). The maximum pump-controlled blood flow at initial dialysis was 232.60 ± 16.72 ml/min in PTA group, which was significantly higher than 197.11 ± 10.45 ml/min in reconstruction group (p < .010). Subcutaneous hematoma, restenosis, thrombus formation, and pseudoaneurysm were major complications in PTA group. Restenosis, thrombus formation, and pseudoaneurysm were major complications in reconstruction groups, with no statistically significant differences between the two groups (p > .050).
Conclusion
The efficacy of PTA is similar to that of arteriovenous fistula reconstruction for immature arteriovenous fistula. While, PTA can dramatically reduce the maturation time, and increase the maximum pump-controlled blood flow at initial dialysis after operation.
Developing a whole exome sequencing-based homologous recombination deficiency test of epithelial ovarian cancer
Authors: Lin Po-Han , Kuo Kuan-Ting , Hwu Wuh-Liang , Huang Hsien-Neng , Lin Tzu-Ying , Chen Chieh-Min , Cheng Wen-Fang , Chiang Ying-Cheng 
Published: May 2023

Abstract
Background:
Homologous recombination deficiency (HRD) test is an important tool to stratify epithelial ovarian cancer (EOC) patients for maintenance therapy. Using whole exome sequencing (WES)-based platform can provide information of gene mutations and HRD score, however, the clinical value of WES-based HRD test was less validated in EOC.
Methods:
We evaluated the performance of WES-based HRD test by using scarHRD software (https://github.com/sztup/scarHRD) in 44 EOC patients. Samples were concordantly examined using Myriad myChoice® and ACT Genomics. The correlation between HRD status and clinical outcomes was analyzed among the three tests.
Results:
A high correlation of HRD scores was observed between our WES-based scarHRD test and Myriad (coefficient 0.82, p < 0.001). Compared with positive HRD status in Myriad test, our WES-based scarHRD test had sensitivity, specificity, positive predictive value, and negative predictive value of 93.5%, 76.9%, 90.6%, and 83.3%, respectively. Positive HRD status by our WES-based scarHRD test and Myriad test both were highly associated with advanced FIGO stage and sensitive platinum-response. In multivariate Cox regression analysis, optimal debulking surgery (hazards ratio [HR] 0.39, 95% confidence interval [CI] 0.18–0.84, p = 0.017) and positive HRD status of our test (HR 0.42, 95% CI 0.20–0.90, p = 0.026) were independent factors for the lower risk of disease recurrence. However, the positive HRD status either by Myriad or ACT genomics was not significantly associated with an inferior trend of recurrence.
Conclusions
Our WES-based scarHRD test provides comprehensive information about gene mutations and HRD scores. It is a new feasible option to determine the HRD status in EOC patients.
Genetically determined watercress intake and risk for bone mineral density
Authors: dai zhicheng , Lin Longshuai , Liu Yuan , He Enjun , guo weihong , Chen Chen , Guouo Zengya , Wang Hongjie , zhao qinghua 
Published: May 2023

Abstract
Purpose: It has been previously shown that watercress intake increases the expression of type I collagen and promotes human osteoblast-like MG-63 cells of osteoblastic proliferation and differentiation. However, there have been no randomized controlled clinical trials of watercress intake in bone mineral density (BMD). It remains to be clarified if watercress intake leads to an increase in BMD. In this study, we used two-sample Mendelian randomization (MR) to estimate the causal effect of watercress intake on susceptibility to BMD.
Methods: Single nucleotide polymorphisms (SNP) associated with watercress intake phenotypes, used as instrumental variables, were extracted from public genome-wide association study (GWAS) summary statistics. We take the intake of watercress as the exposure and BMD as the outcome. By using MR, it is possible to investigate whether there is a causal association between the exposure and the outcome.
Results: Using the IVW-fixed(Inverse variance weighted, fixed effects) and IVW-random methods(Inverse variance weighted, multiplicative random effects), we found the evidence to report the causal associations between the watercress intake and femoral neck BMD , but the intake of watercress has nothing to do with lumbar spine BMD and forearm BMD（Table 1）.
Conclusion: The present study suggested a harmful role of watercress intake on the risk for femoral neck BMD. Further studies elucidating the underlying mechanism are warranted.
Relationship between autonomic nervous function and brain functions such as memory and attention
Authors: Tatsumi Sakura , Kuratsune Daisuke , Kuratsune Hirohiko 
Published: May 2023

Abstract
The present study aimed to evaluate the relationship between autonomic nervous function and brain functions, such as memory and attention, during the kana picking test (KPT). Eleven female college students (21 years old) participated in the study. Each participant performed the KPT 10 times while their autonomic nervous function was analyzed using heart rate variability (HRV) analysis. Autonomic function was assessed at four time intervals with participants' eyes open. In the KPT, participants were asked to circle Japanese vowels in a 400-character story written in Hiragana, and attention was evaluated using the "kana picking score" (KPS). In the memory test, comprehension and memory were evaluated based on 10 descriptive questions about the content of the story. The results showed that the higher the KPS, the higher the memory scores (r = 0.309, p < 0.01). The log low-frequency component power (LF), log high-frequency component power (HF), log (LF + HF), and log LF/HF scores significantly decreased during the interval B. Log LF, log HF, and log (LF + HF) recovered during the interval C, and log LF/HF recovered during the interval D. The KPS had a positive correlation with log LF and log (LF + HF) at intervals A, C, and D. Additionally, the KPS was positively correlated with log HF at all intervals. However, at interval B, the KPS was negatively correlated with log LF/HF. Memory scores showed a positive correlation with log LF, log HF and log (LF + HF) at all intervals and a negative correlation with log LF/HF at all intervals. Furthermore, memory scores were negatively correlated with the amounts of changes of Δlog LF, Δlog HF and Δlog (LF + HF) between intervals B and C. These results indicate that autonomic nervous function is related to learning situations, such as attention, comprehension, memory, and memory recall. Therefore, the assessment of autonomic nervous function could be useful for evaluating learning and work efficiency.
Based on Aptamer-carbon quantum dots and silver nanoparticles FRET sensor for sensitive detection of E. coli
Authors: Bai Xiaolian , Ga Lu , Ai Jun 
Published: May 2023

Abstract
In this work, carbon quantum dots were prepared from grapefruit peel as carbon source by microwave heating method. The prepared carbon quantum dots CQDs were analyzed by fluorescence spectroscopy, TEM analysis, XPS analysis, etc., as well as their optical properties were also investigated. The prepared CQDs have high green fluorescence with excitation wavelength of 320 nm and maximum emission wavelength of 415 nm. the average particle size is about 7.4 nm, uniform dispersion and good stability. And a FRET (fluorescence resonance energy transfer) based fluorescence method was constructed by combining carbon quantum dots with silver nanoparticles (Ag NPs) for the rapid detection of E. coli. Its fluorescence transduction is based on the spectral overlap between the donor (CQDs) emission and the acceptor (nanoparticles) absorbance. The fluorescence of the aptamer-attached CQDs is burst in the presence of and silver nanoparticles. Upon addition of the specific E. coli solution, an aptamer-target complex is formed and the preferential interaction of the aptamer with the specific bacteria leads to the release of CQDs and Ag NPs. After incubation time, the bacterial cells are centrifuged, leading to the precipitation of E. coli aptamer couples and Ag NPs, resulting in the recovery of CQDs fluorescence. This method allows specific detection of E. coli in a wide range of pathogenic bacteria. The final results showed that the linear range of the sensor was 2×103 ~ 2×108 CFU·mL− 1 and the detection limit for E. coli was as low as 77 CFU·mL− 1.
Optimization method of college students' entrepreneurial path based on improved multi-objective gray wolf algorithm
Authors: Qiu Baorong 
Published: May 2023

Abstract
Fromperspectives, and they can be classified into three categories according to different viewpoints: "platform theory", that is, the entrepreneurial environment is regarded as the government and society for entrepreneurs to create new A public platform built by enterprises; "factor theory", that is, the entrepreneurial environment is understood as a combination of various factors affecting entrepreneurial behavior; "system theory", that is, the entrepreneurial environment is regarded as a complex integrated into various entrepreneurial environment elements. The system believes that the entrepreneurial environment refers to the situation around the entrepreneur, which is of course, because the connotation and extension of the entrepreneurial environment are very rich, it is difficult to accurately define its concept. Drawing on the viewpoints of "factor theory" and "system theory", the author believes development of college students and their innovative careers.With the advent of the Internet era, more and more college students use the Internet to start businesses. If college students want to realize their own value through entrepreneurship, they need to choose their own development path. The optimization of it, namely, the optimization of entrepreneurial opportunities, the optimization of entrepreneurial resources, and the optimization of entrepreneurial teams. In order proposes two improvement strategies based on the analysis of the individual movement of the gray wolf when the algorithm is optimized. The second is to improve the control parameter adjustment strategy, and use the power function instead of the linear function. This paper analyzes choice by optimizing the entrepreneurial path to further improve the entrepreneurialone, it provides a favorable environment.