bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enzyme-Instructed Peptide Self-Assembly as A Cell Membrane Lichen Activating Macrophage-Mediated Cancer Immunotherapy
Authors: Cai Yanbin 
Published: September 2023

Abstract
Targeted immunomodulation for reactivating innate cells, especially macrophages, holds great promise to complement current adaptive immunotherapy. Nevertheless, there is still a lack of high-performance inhibitors for blocking macrophage phagocytosis checkpoints in immune quiescent solid tumors so far. Herein, a peptide-antibody combo-supramolecular in situ assembled CD47 and CD24 bi-target inhibitor (PAC-SABI) is described, which undergoes biomimetic surface propagation like lichens on cancer cell membranes through ligand-receptor binding and enzyme-triggered reactions. Primarily, the PAC-SABIs demonstrate specific avidity for the overexpressed CD24 on the cancer cell surface with anti-CD24 monoclonal antibody (mAb). Subsequently, they exhibit alkaline phosphatase-catalyzed rapid dephosphorylation of phosphopeptides, constructing a three-dimensional nanofiber network and reinstating blockade of CD47 signaling. By concurrent inhibition of CD47 and CD24 signaling, PAC-SABIs stimulate macrophage phagocytosis and initiate T cell antitumor response. Remarkably, compared with anti-CD24 mAb, PAC-SABIs enhance the phagocytic ability of macrophages by 3–4 times in vitro and in vivo while facilitating infiltration of CD8+ T cells into 4T1 tumors. Moreover, combining PAC-SABIs with anti-PD-1 therapy effectively suppressed 4T1 tumor growth in murine models, surmounting other treatment groups with a 60-day survival rate of 57%. The in vivo construction of PAC-SABI-based nanoarchitectonics provides an efficient platform for bridging innate and adaptive immunity to maximize therapeutic potency.
Genetically divergent shallow and mesophotic Stephanocoenia intersepta coral populations across Florida Keys National Marine Sanctuary
Authors: Eckert Ryan , Sturm Alexis , Carreiro Ashley , Klein Allison , Voss Joshua 
Published: September 2023

Abstract

Population genetic analyses can provide useful data on species’ regional connectivity and diversity which can inform conservation and restoration efforts. In this study, we quantified the genetic connectivity and diversity of Stephanocoenia intersepta corals from shallow (<30 m) to mesophotic (30–45 m) depths across Florida Keys National Marine Sanctuary. We generated single nucleotide polymorphism (SNP) markers to identify genetic structuring of shallow and mesophotic S. intersepta populations. We uncovered four distinct genetic lineages with varying levels of depth-specificity. Shallow lineages exhibited lower heterozygosity and higher inbreeding relative to depth-generalist lineages found predominantly on mesophotic reefs. Estimation of recent genetic migration rates demonstrated that mesophotic populations are more prolific sources than shallow populations, particularly in the Lower Keys and Tortugas Bank. Additionally, we compared endosymbiotic Symbiodiniaceae communities among S. intersepta populations using the ITS2 region and SymPortal analysis framework, identifying symbionts from the genera Symbiodinium, Breviolum, and Cladocopium. Symbiodiniaceae communities varied significantly across depth and location and exhibited significant, but weak correlation with host population structure and genotype. Together, these data demonstrate that despite population genetic structuring across depth, mesophotic populations may provide refuge for shallow populations moving forward and remain important contributors to the overall genetic diversity of this species throughout the region. This study highlights the importance of including mesophotic as well as shallow coral populations in population genetic assessments and informs future science-based management, conservation, and restoration efforts within Florida Keys National Marine Sanctuary.
Mettl1-dependent m7G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster
Authors: Saito Kuniaki 
Published: September 2023

Abstract

N7-methylguanosine (m7G) in the variable loop region of tRNA is catalyzed by METTL1/WDR4 heterodimer and stabilizes target tRNA. Here, we reveal essential functions of Mettl1 in Drosophila fertility. Knockout of Mettl1 (Mettl1-KO) lost the elongated spermatids and mature sperm, which was fully rescued by a Mettl1-transgene expression, but not a catalytic-dead Mettl1 transgene. This demonstrates that Mettl1-dependent m7G is required for spermatogenesis. Mettl1-KO resulted in a loss of m7G modification on a subset of tRNAs and a decreased level of tRNA expression. Strikingly, overexpression of the translational elongation factor, EF1α1, which can compete with the rapid tRNA decay (RTD) pathway in S. cerevisiae, significantly counteracted the sterility of Mettl1-KO males, supporting a critical role of m7G modification of tRNAs in spermatogenesis. Ribosome profiling showed that Mettl1-KO led to the ribosome stalling at codons decoded by tRNAs that were reduced in expression. Mettl1-KO also significantly reduced the translation efficiency of genes involved in elongated spermatid formation and sperm stability. These findings reveal a developmental role for m7G tRNA modifications and indicate that m7G modification-dependent tRNA stability differs among tissues.
Prevalence and Predictors of Erectile Dysfunction among Filipino men: A cross-sectional study
Authors: De Guzman Sagie 
Published: September 2023

Abstract
Objective: This study aimed to estimate the prevalence and predictors of Erectile Dysfunction (ED) among Filipino men 18 years and older using a cross-sectional survey design.Methods: A sample of N = 1, 246 men was recruited from multiple regions across the Philippines using social media platform. The International Index of Erectile Function (IIEF) questionnaire was administered to assess the degree of ED. Additional demographic data were collected to evaluate the impact of variables such as age, educational level, civil status, and sexual orientation on the prevalence of ED.Results: The overall prevalence of ED among Filipino men was found to be 78.8%. Age is an independent significant predictor (ß= .289, t = 6.65, p < .001), with young adult men aged 18–39 showing a higher prevalence (45.3%) compared with middle-aged, 40–64 years (26.3%). Factors such as age, educational level, and civil status were also significantly associated with higher rates of ED. Interestingly, social and cultural variables, such as marital status and educational level, showed varying influences on the prevalence rates.Conclusions: This study is one of the first comprehensive investigations into the prevalence of ED among Filipino men. The findings not only fill a critical gap in the existing literature but also offer sociocultural insights that may be crucial for future interventions and public health planning. The primary care providers are at the best position to screen and assess young and middle-aged Filipino men for ED to prompt early management and timely referral. Further studies are needed to investigate the facilitators and barriers of health-seeking behaviors among Filipino men concerning ED.
The Impact of the Pre-gradual Course on Professional Communication, Prevention of Violence and Self-Protection on Early Paramedics Practice: A Qualitative Study
Authors: Pekara Jaroslav , Kočovská Eva , Sýkora Roman , Němcová Jitka , Vlk Radomír 
Published: September 2023

Abstract
Background Violence against paramedics has been described as a serious public health problem with the potential for significant physical and psychological harm. Education and training have been identified as key elements of any workplace violence prevention program. To compare the influence of the pregraduate course on 'Professional Communication and Prevention of Violence' and 'Self-Protection' on factors that impacted prevention, communication and understanding of violent patients among paramedics in their early practice.
Method This qualitative study was a form of thematic analysis and adopted an inductive, ‘bottom-up’ approach, in which coded categories were derived from rich, descriptive data. Twenty semistructured in-depth interviews were carried out with paramedics two years after their graduation. The outcomes from the intervention group (course graduates, n = 10) were compared with a control group (did not attend the course, n = 10) matched for gender, age, specialisation, level of education, and practice experience.
Results The course graduates were more likely to consider the causes and reasons leading to patients’ violence than the control group. Moreover, course graduates tried to prevent conflict more often and demonstrated greater respect for, compassion for and understanding violent patients.
Conclusion This study demonstrates that thorough, deep and focused pre-graduate education focused on communication is more likely to lead to consideration of the causes and reasons leading to patients’ violence in early paramedic practice.
Fully flexible implantable neural probes for electrophysiology recording and controlled neurochemical modulation
Authors: Chamanzar Maysamreza , Malekoshoaraie Mohammad , Wu Bingchen , Krahe Daniela , Ahmed Zabir , Pupa Stephen , Jain Vishal , Cui Xinyan 
Published: September 2023

Abstract

Targeted delivery of neurochemicals and biomolecules for neuromodulation of brain activity is a powerful technique that, in addition to electrical recording and stimulation, enables a more thorough investigation of neural circuit dynamics. We have designed a novel flexible neural implant capable of controlled, localized chemical stimulation and electrophysiology recording. To minimize tissue damage and response, the neural probe was implemented with a small cross-sectional dimension using planar micromachining processes on Parylene C, a mechanically flexible, biocompatible substrate. The probe shank features two large microelectrodes (chemical sites) for drug loading and sixteen small microelectrodes for electrophysiology recording to monitor neuronal response to drug release. To reduce the impedance while keeping the size of the microelectrodes small, poly(3,4-ethylenedioxythiophene) (PEDOT) was electrochemically coated on recording microelectrodes. In addition, PEDOT doped with mesoporous sulfonated silica nanoparticles (SNP) was used on chemical sites to achieve controlled, electrically-actuated drug loading and releasing. Different neurotransmitters, including glutamate (Glu), and gamma-aminobutyric acid (GABA), were incorporated into the SNPs and electrically triggered to release repeatedly. An in vitro experiment was conducted to quantify the stimulated release profile by applying a sinusoidal voltage (0.5 V, 2 Hz). The flexible neural probe was implanted in the barrel cortex of the wild-type Sprague Dawley rats. As expected due to their excitatory and inhibitory effects, Glu and GABA release caused a significant increase and decrease in neural activity, respectively, which was recorded by the recording microelectrodes. This novel flexible neural probe technology, combining on-demand chemical release and high-resolution electrophysiology recording, is an important addition to the neuroscience toolset used to dissect neural circuitry and investigate neural network connectivity.
Cyclic-di-AMP confers an invasive phenotype on Escherichia coli through elongation of flagellin filaments
Authors: Tanaka Rika , Imai Jin , Sugiyama Eiji , Tsubaki Shogo , Hozumi Katsuto , Tsugawa Hitoshi 
Published: September 2023

Abstract
Adherent-invasive Escherichia coli (AIEC) is isolated from patients with Crohn’s disease (CD). AIEC can invade the intestinal epithelium, suggesting that it is involved in the development and pathogenesis of CD. However, themechanism by which AIEC acquired the invasive phenotype remains unknown. This study was designed to examine the mechanisms of AIEC invasiveness. We found that the flagellin (fliC) expression in AIEC was two-fold higher than that in non-AIEC strains, and this overexpression induced the formation of long-filament flagellin. Deletion of fliC in the AIEC LF82 strain resulted in the disappearance of flagellar filamentsand attenuated the motility and invasive ability of the bacterium, suggesting that the formation of long filament flagellin induced by increased fliC expression is required by AIEC to invade the intestinal epithelium. In AIEC and non-AIEC K12 strains cultured in the presence of cyclic-di-AMP (c-di-AMP), the expression of fliC was enhanced, and flagellar filaments were elongated. Stimulation with c-di-AMP enhanced the bacterial motility and ability to invade epithelial cells, even in the non-AIEC K12 strain. Our findings show that c-di-AMP confers an AIEC-like phenotype on non-AIEC strains by enhancing the expression of fliC. The results should be useful for understanding the pathogenesis of CD.
CD146-assisted isolation and multi-omics characterisation of human pluripotent stem cell derived cranial Schwann cell precursor-like cells.
Authors: Wolvetang Ernst , Balachandran Anushree , Dave Keyur , Tiwari Kanupriya , Gomez-Inclan Cecilia , Leeson Hannah C. , Su Shian , Pietrogrande Giovanni , Ovchinnikov Dmitry , Hawksworth Owen , Amann-Zalcenstein Daniela , Voss Carola , Wells Christine , Headlam Madeleine. , Cooper-White Justin , Glass Nick , Stefani Ilaria , Tull Dedreia , Schittenhelm Ralf , Marchant Ceilidh , Schwarz Quenten , De Souza David , McConville Malcolm , Nielsen Lars , Gorman Jeff , Naik Shalin 
Published: September 2023

Abstract
Schwann Cell Precursors (SCPs) are multipotent precursor cells and express SOX10, but relatively little is known about the specification and molecular make-up of human SCPs. To address this, we subjected a human SOX10 knock-in reporter iPSC line to a one step cranial Schwann cell precursor differentiation protocol. We show that SOX10-expressing cells acquire the morphology, motility and gene expression of SCP-like cells, and we exemplify that these cells migrate to multiple developing craniofacial structures following injection into early mouse embryos. We next defined the bulk and single-cell transcriptomes, metabolomes, and proteomes of SOX10 expressing human SCP-like cells, revealing gene expression heterogeneity, shifts in splicing events, a reduced dependence on glycolysis, and changes in the expression of cell adhesion proteins, miRNAs and LncRNAs that accompany SCP specification. Discovery proteomics identifies MCAM (CD146) as a cell surface marker that permits the isolation of pure SOX10 expressing human cranial SCP-like cells from human pluripotent stem cell lines subjected to SCP differentiation. We further show that this permits isolation of Down syndrome cranial SCP-like cells that display defects in proliferation. Collectively these data provide a detailed map of the molecular make-up of in vitro generated human cranial Schwann cell precursor-like cells and exemplify the utility of MCAM-sorted cranial SCP-like cells for modelling human neurocristopathies.
Timing of physical therapy to optimize shoulder range of motion among patients receiving breast radiotherapy
Authors: Baek Carolyn , Saraf Anurag , Hu Bonnie , Specht Michelle C. , Liao Eric C , Jimenez Rachel B. 
Published: September 2023

Abstract

Purpose
Establishing optimal timing of physical therapy (PT) during multimodal breast cancer treatment can be challenging. We hypothesized patients initiating PT post-surgery and pre-radiation therapy (RT) would experience greater gains in functionality compared to PT initiated during/after RT.
Methods
A retrospective review was performed for patients receiving multimodality breast cancer treatment and PT between 1/2015-8/2021. Three cohorts were established: patients who received no RT, PT initiated before RT (pre-RT), and PT during/after RT (d/a-RT). The primary endpoint was percent change in ipsilateral shoulder ROM between first and last PT visits. Associations between range of motion (ROM) across groups and baseline characteristics were evaluated with analysis of variance testing.
Results
Thirty-seven patients were identified, median age of 47 years. The no-RT cohort exhibited the greatest mean percent improvement in ROM with PT (74%) versus pre-RT (59%) and d/a-RT (20%). The no RT and pre-RT groups demonstrated greater improvements in ROM compared to patients receiving PT d/a-RT (no RT 74% vs d/a-RT 20%, p = 0.006; pre-RT 59% vs d/a-RT 20%, p = 0.045). There was no difference in ROM gains between the no-RT and pre-RT groups (p = 0.528). Lower baseline ROM was associated with smaller improvements in ROM after PT (p = 0.008). When adjusting for baseline ROM, the no-RT cohort remained associated with ROM improvement compared to the d/a-RT group (p = 0.024). There was no difference in ROM change between no-RT and pre-RT cohorts.
Conclusion
PT improves shoulder ROM in patients undergoing multimodality breast cancer treatment; however, early initiation of PT before starting RT may maximize ROM gains.
Alpha-mangostin promotes diabetic wound healing: An in vitro study with mechanistic elucidation
Authors: Patrick Melonney , Zohdi Wan Najwa Wan Mohd , Muid Suhaila Abd. , Omar Effat 
Published: September 2023

Abstract
Poor wound healing is a common manifestation of diabetes mellitus, culminating in chronic, non-healing ulcer. Alpha(α)-mangostin, one of the most active xanthones found in mangosteen pericarp, has been reported to promote wound healing. However, its effectiveness and mechanism in expediting diabetic wound healing is unknown. The aim of this study was to investigate the effect of alpha-mangostin on wound cell migration and growth factor expressions in a diabetic wound healing model. Human coronary artery endothelial cells (HCAEC) and human dermal fibroblast (HDF) cells were used in this laboratory study. Alpha-mangostin of different concentrations and carboxymethyl cellulose (used as positive control) were introduced to the cell culture plates. Scratch assay was performed for each plate and the rate of cell migration was calculated. Growth factors released by the cells were measured using the ELISA method. Treatment with alpha-mangostin at 0.15 ug/ml concentration showed the fastest rate of endothelial and fibroblast cell migration compared to negative controls. Alpha-mangostin treatment increased PDGF, TGF-β, FGF, TIMP, and reduced MMP-9 levels compared to glucose controls. The findings indicate that in an in vitro diabetic wound healing model, alpha-mangostin stimulates endothelial and fibroblast cell migration, increased the release of growth factors, and lowered the MMP-9 secretion.
Efficacy and safety of Huachansu combined with adjuvant chemotherapy in resected colorectal cancer patients: A prospective, open label, randomized phase II study
Authors: Li Shichao , Shen Dongxiao , Zuo Qingsong , Wang Shengzi , Meng Lingjia , Yu Jiaojiao , Liu Yang , Li Wusheng , Chen Chao , Yin Peihao , Chen Teng , Wang Jie 
Published: September 2023

Abstract
Background
Although some studies in China have suggested Huachansu (HCS) combined with chemotherapy is effective in the treatment of various cancers, there are few studies on colorectal cancer (CRC), especially in postoperative adjuvant chemotherapy. The aim of this study was to test the hypothesis that HCS combined with adjuvant chemotherapy would improve survival probability in resected CRC patients.
Methods
This was a prospective, open label, randomized phase II study. Patients with stage III or high-risk stage II resected CRC were randomly assigned to the chemotherapy and HCS + chemotherapy groups. The Chemotherapy group was treated with the FOLFOX regimen for ≥ 6 cycles or the CAPEOX regimen for ≥ 4 cycles. The HCS + chemotherapy group was treated with HCS on the basis of the chemotherapy group. The primary endpoint was 3-year disease-free survival (DFS), and the secondary endpoints were 3-year overall survival (OS) and toxicity.
Results
A total of 250 patients were included in this study (126 chemotherapy, 124 HCS + chemotherapy). There were significant differences in 3-year DFS between the two groups (median 28.7 versus 31.6 months respectively; P = 0.027), but no significant differences in 3-year OS between the two groups (median 32.7 versus 34 months, respectively; P = 0.146). No patients experienced grade 4 adverse events, and the rates of leukopenia, neutropenia and diarrhea in the HCS + chemotherapy group were lower than that those in the chemotherapy group.
Conclusion
HCS combined with adjuvant chemotherapy after radical resection for patients with stage III or high-risk stage II CRC was demonstrated to be an effective and feasible treatment.
Complement Factor H attenuates TNF-α-induced inflammation by upregulating EIF3C in rheumatoid arthritis
Authors: Jia Yimeng , Feng Bin , Ji Xin , Tian Xinping , Zhao Lidan , Zhou Jiaxin , Zhang Wen , Li Mengtao , Fei Yunyun , Wu Xunyao 
Published: September 2023

Abstract
Objective
To explore the role and underlying mechanism of Complement Factor H (CFH) in the peripheral and joint inflammation of RA patients.
Methods
The levels of CFH in the serum and synovial fluid were determined by ELISA. The pyroptosis of monocytes was determined by western blotting. The inflammation cytokine release was tested by ELISA. The cell migration and invasion ability of fibroblast-like synoviocyte (FLS) were tested by Wound healing Assay and transwell assay, respectively. The potential target of CFH was identified by RNA sequencing.
Results
CFH levels were significantly elevated in the serum and synovial fluid from RA and associated with high sensitivity C-reactive protein (hs-CRP), erythrocyte sedimentation rate (ESR), and disease activity score 28 (DAS28). TNF-α could inhibit CFH expression, and CFH combined with TNF-α significantly decreased cleaved-caspase 3, gasdermin E N-terminal (GSDME-N), and inflammatory cytokines release (IL-1β and IL-6) of RA-derived monocytes. Stimulated with TNF-α increased CFH levels in RA FLS and CFH inhibits the migration, invasion and TNF-α–induced production of inflammatory mediators, including proinflammatory cytokines (IL-6, IL-8) as well as matrix metalloproteinases (MMPs, MMP1 and MMP3) of RA FLSs. The RNA-seq results showed that CFH treatment induced upregulation of eukaryotic translation initiation factor 3 (EIF3C) and the migration of RA FLSs was promoted and the expressions of IL-6, IL-8, and MMP-3 were enhanced upon EIF3C knockdown under the stimulation of CFH combined with TNF-α.
Conclusion
In conclusion, we have unfolded the anti-inflammatory roles of CFH in the peripheral and joints of RA, which might provide a potential therapeutic target for RA patients.
Relationship between degenerative changes in the lumbar spine and sacroiliac joint in patients with total hip arthroplasty: Possibility of Hip-Sacroiliac Joint-Spine syndrome
Authors: Kaneuji Ayumi , Fukui Makoto , Takahashi Eiji , Sanji Yusuke , Hirata Hiroaki , Kawahara Norio 
Published: September 2023

Abstract
This study is designed to compare the extent of sacroiliac joint (SIJ) degeneration at total hip arthroplasty (THA) for two pathologies: osteoarthritis of the hip (OA) and osteonecrosis of the femoral head (ON). We also assessed the prevalence of SIJ degeneration in patients with lumbar spondylolisthesis or degenerative scoliosis. A total of 138 hips from 138 patients (69 OA and 69 ON) were assessed in this study, including 66 hips affected by OA secondary to developmental dysplasia of the hip. The degenerative changes in the SIJ and lumbar spine were evaluated prior to THA using radiographs and computed tomography (CT) scans, showing 9 instances of spondylolisthesis and 38 of degenerative scoliosis. The OA group exhibited longer duration from onset to surgery than the ON group. The OA group also included more cases with significant pelvic obliquity (3 degrees or more) and with significant increases in SIJ sclerosis and irregularities. Patients with lumbar spondylolisthesis or degenerative scoliosis were significantly more likely to have SIJ irregularities. The prevalence of SIJ degeneration was higher in cases of THA for OA than for ON. This study also suggests the possibility of Hip-SIJ-Spine syndrome in THA patients with OA.