bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful use of HTF as a basal fertilization medium during SEcuRe mouse in vitro fertilization
Authors: Wigger Magdalena , Schneider Marco , Feldmann Anni , Assenmacher Sonja , Zevnik Branko , Tröder Simon E. 
Published: July 2023

Abstract
Objective: The ever-increasing number of genetically engineered mouse models highlights the need for efficient archiving and distribution of these lines. Sperm cryopreservation has become the preferred technique for the majority of these models due to its low requirement of costs, time and experimental animals. Yet, current in vitro fertilization (IVF) protocols either exhibit decreased fertilization efficiency for the most popular C57BL/6 strain, as recently demonstrated by us, or require costly and difficult-to-prepare media, respectively. As a result, we previously developed SEcuRe, a modified IVF protocol with low costs and high fertilization efficiency. The popular basal fertilization medium, Cook’s® proprietary “Research Vitro Fert” (RVF), used in this protocol has recently been discontinued. As a result, the application of the SEcuRe approach and other IVF protocols employing this medium has been severely limited.
Results: Here we show that human tubal fluid (HTF), a popular and widely available medium with a known composition, can be used as a basal fertilization medium instead of RVF. Comparison of RVF and HTF during 58 independent SEcuRe IVFs with cryopreserved C57BL/6 sperm revealed equal fertilization rates. In addition, we demonstrate that HTF has a substantially extended shelf-life by utilizing commercial HTF that was six months past its expiration date, yet did not affect fertilization during IVF or subsequent embryo development. This finding not only increases the economic value of our modified method, but also validates it once more. Our results demonstrate that common, shelf-life extended HTF can be used in SEcuRe IVF in place of now-discontinued RVF medium and ensure the applicability of the method, which we since termed SEcuRe 2.0. Our modified SEcuRe 2.0 strategy will assist researchers to efficiently archive and distribute genetically engineered mouse models in a cost-effective, easily adaptable, and 3R-compliant manner with minimal animal use.
Lens subluxation combined with Parry-Romberg Syndrome and a mini review
Authors: Tang Yating , Jing Qinghe , Jiang Yongxiang , Lu Yi 
Published: July 2023

Abstract
Introduction:
Parry-Romberg syndrome (PRS) is a rare progressive degenerative disorder of unknown etiology. Here we report a rare case of PRS combined with lens subluxation in Eye and ENT hospital of Fudan University, Shanghai. To our knowledge, it is the first reported case of PRS combined with lens subluxation that has been managed surgically with phacoemulsification and CTR placement and IOL implantation in Shanghai.
Patient and clinical findings:
A 60-year-old woman was referred for “right visual blur for 2 years” and had persistent right facial paralysis of unknown etiology since the age 12. She had right facial muscle atrophy and paralysis. Eye examination also showed the right eyelid pseudoptosis, enophthalmos, age-related cataract combined with lens subluxation existed in the right eye.
Diagnosis, intervention, and outcomes:
The patient was diagnosed as age-related cataract and lens subluxation in the right eye and progressive hemifacial atrophy (Parry-Romberg syndrome). We conducted a combined phacoemulsification, IOL and CTR implantation and pupilplasty surgery for the patient under general anesthesia and the postoperative UCVA was 20/30 and remained for 1 year’s follow up.
Conclusions
Here we reported a rare case of PHA combined with lens subluxation in China. After appropriate eye surgery, the patient achieved satisfying vision result in the right eye.
From colonial clusters to colonial sheaths: analysis of Microcystis morphospecies in mesocosm by imaging flow cytometry
Authors: Zhumakhanova Adina , Mirasbekov Yersultan , Malashenkov Dmitry V. , Davidson Thomas A. , Levi Eti Ester , Jeppesen Erik , Barteneva Natasha S. 
Published: July 2023

Abstract
The alarming increase in the frequency of blooms of Microcystis in freshwater lakes and reservoirs occurs worldwide, with major implications for their ecosystem functioning and water quality. We applied FlowCAM-based imaging flow cytometry together with PCR and sequencing to get a comprehensive picture of the seasonal development of Microcystis community in a long-term running lake mesocosm experiment. The IFC analysis with manual taxonomic classification confirmed early findings with a machine learning algorithm that some Microcystis morphospecies completely disappeared and re-appeared along the mesocosm experiment timeline. This observation supports the hypothesis of the main transition pathways of colonial Microcystis. For the first time, colonial mucilaginous envelopes or sheaths were reported as separate entities, and not as a part of Microcystis colonies. The colonial sheaths may contain a few single Microcystis cells and reach significant numbers (thousands) during a cyanobacterial bloom. We also found that non-identifiable colonial small clusters of Microcystis cells are an important stage in the complex mosaic of a Microcystis bloom and are associated with the development of colonial forms. Our findings were validated by the principal component analysis coupled with the constructed associative matrices. We hypothesize that colonial sheaths may be crucial at Microcystis spp. dispersal and represent one of the stages of colonies development.
Geographic distribution and impacts of climate change on the suitable habitats of Rhamnus utilis in China
Authors: guiquan Song , Jiali Feng , shuai Gong , wenya Hao , xiangkun Kong , Sheng Zhao , yueling Zhao , xuelian Jiang 
Published: July 2023

Abstract
Rhamnus utilis (Rhamnaceae) is an ecologically and economically important tree species. The growing market demands and recent anthropogenic impacts to R. utilis forests has negatively impacted its populations severely. However, little is known about the potential distribution of this species and environmental factors that affect habitat suitability for this species. By using 219 occurrence records along with 51 environmental factors, present and future suitable habitat were estimated for R. utilis using MaxEnt modelling; the important environmental factors affecting its distribution were analyzed. The results indicate that January water vapor pressure, the normalized difference vegetation index, mean diurnal range, and precipitation of the warmest quarter represented the critical factors explaining the environmental requirements of R. utilis. The potential habitat of R. utilis included most provinces from central to southeast China. Under climate change scenario SSP 245, MaxEnt predicted a cumulative loss of ca. 0.73 × 105 km2 for suitable habitat for R. utilis by the 2060s while an increase of ca. 0.65 × 105 km2 occurred in the 2010s. Furthermore, under this climate change scenario, the suitable habitat will geographically expand to higher elevations. The findings of our study provide a foundation for targeted conservation efforts and inform future research on R. utilis. By considering the identified environmental factors and anticipating the potential impacts of climate change, conservation strategies can be developed to preserve and restore suitable habitats for R. utilis. Protecting this species is not only crucial for maintaining biodiversity but also for sustaining the economic benefits associated with its ecological services.
Exploring challenges and best practice in Memory Assessment Services in England and Wales – a national survey and case study approach
Authors: Smith Sarah Jane , Surr Claire 
Published: July 2023

Abstract

Background: To explore the design, delivery models and identify good and innovative practices in Memory Assessment Services (MAS) in England and Wales.
Methods: A two-stage service evaluation comprising 1) on-line survey of MAS providers to identify features of the commissioning models, service design, delivery, and challenges alongside examples of good/innovative practice; 2) qualitative case studies using video/telephone interviews with key staff and people who had used the service.
Results: The 49 respondents to the survey reported a shift in delivery of MAS services post COVID and identified key areas for improvement, including need for specialist staff, support for MCI and rarer dementias, and capacity for post diagnostic support. The 15 case studies illustrated good practice and innovation focusing on post diagnostic support, equity of access, working with external services/service location, MCI and rarer dementia and involving specialist staff.
Conclusions: The evaluation speaks to the importance of (re)evaluation of services to identify local need and the importance of commissioning based on local need and innovative approaches that my sit outside of ‘typical’ MAS pathways.
2D Capsule Networks Detect Perceived Changes in Infant∼Environment Relationship Reflected in 3D Movement Dynamics
Authors: khodadadzadeh Massoud , Sloan Aliza T. , Jones Nancy Aaron , Coyle Damien , Kelso J. A. Scott 
Published: July 2023

Abstract
Can infant exploration and causal discovery be detected using Artificial Intelligence (AI)? A recent experiment probed how purposeful action emerges in early life by manipulating infants’ functional connection to an object in the environment (i.e., tethering one foot to a colorful mobile). Vicon motion capture data from multiple infant joints were used here to create Histograms of Joint Displacements (HJDs) to generate pose-based descriptors for 3D infant spatial trajectories. Using HJDs as inputs, machine and deep learning systems were tasked with classifying the experimental state from which snippets of movement data were sampled. The architectures tested included k-Nearest Neighbour (kNN), Linear Discriminant Analysis (LDA), Fully connected network (FCNet), 1D-Convolutional Neural Network (1D-Conv), 1D-Capsule Network (1D-CapsNet), 2D-Conv and 2D-CapsNet. Sliding window scenarios were used for temporal analysis to search for topological changes in infant movement related to functional context. kNN and LDA achieved higher classification accuracy with single joint features, while deep learning approaches, particularly 2D-CapsNet, achieved higher accuracy on full-body features. For each AI architecture tested, measures of foot activity displayed the most distinct and coherent pattern alterations across different experimental stages (reflected in the highest classification accuracy rate), indicating that interaction with the world impacts the infant behaviour most at the site of organism∼world connection. Pairing theory-driven experimentation with AI tools thus opens a path to developing functionally-relevant assessments of infant behaviour that are likely to be useful in clinical settings.
Employing electrochemically derived pH gradients for Lab-on-PCB protein preconcentration implementations
Authors: Maxted Grace , Estrela Pedro , Moschou Despina 
Published: July 2023

Abstract
Protein preconcentration is an essential sample preparation step when analysing samples where the targeted proteins are in low concentrations, such as bodily fluids as well as water or wastewater. Nonetheless, very few practical implementations of miniaturized protein pre-concentration devices have been demonstrated in practice and even fewer in integration with other microanalytical steps. In this paper we propose for the first time a miniaturized isoelectric focusing-based protein-preconcentration device based on electrochemically derived pH gradients, rather than existing chemical reagent approaches. That way we are reducing the need for additional chemical reagents to zero, whilst enabling the device incorporation in a seamlessly integrated full protein analysis microsystem via Lab-on-PCB technology. We apply our previously presented Lab-on-PCB approach to quantitatively control the pH of a solution at the vicinity of planar electrodes using the electrochemical generation of acid through redox-active self-assembled monolayers. The presented device was comprised of a printed circuit board with an array of gold electrodes which was functionalised with 4-Aminothiophenol; this formed a self-assembled monolayer which was electropolymerised to improve its electrochemical reversibility. Protein preconcentration was performed in two configurations, one of which was open and required the use of a holder to suspend a well of fluid above the electrodes, and another which used microfluidic channels to enclose small volumes of fluid. Reported here is the data for protein preconcentration in both these forms with a quantitative concentration factor shown for the open form and qualitative proof shown for the microfluidic.
RPTOR blockade suppresses brain metastases of NSCLC by interfering the ceramide metabolism via hijacking YY1 binding.
Authors: Lin Ying , Wu Yun , Zhang Qiangzu , Tu Xunwei , Chen Sufang , Pan Junfan , Xu Nengluan , Lin Ming , She Peiwei , Niu Gang , Chen Yusheng , Li Hongru 
Published: July 2023

Abstract
Background: Ceramide metabolism is crucial in the progress of brain metastasis (BM). However, it remains unexplored whether targeting ceramide metabolism may arrest BM.
Methods: RNA sequencing was applied to screen different genes in primary and metastatic foci and whole-exome sequencing (WES) to seek crucial abnormal pathway in BM+ and BM-patients. Cellular arrays were applied to analyze the permeability of blood-brain barrier (BBB) and the activation or inhibition of pathway. Database and Co-Immunoprecipitation (Co-IP) assay were adopted to verify the protein-protein interaction. Xenograft and zebrafish model were further employed to verify the cellular results.
Results: RNA sequencing and WES reported the involvement of RPTOR and ceramide metabolism in BM progress. RPTORwas significantly upregulated in BM foci and increased the permeability of BBB, while RPTOR deficiency attenuated the cell invasiveness and protected extracellular matrix. Exogenous RPTOR boosted the SPHK2/S1P/STAT3 cascades by binding YY1, in which YY1 bound to the regions of SPHK2 promoter (at -353 ~ -365 nt), further promoting the expression of SPHK2. The latter was rescued by YY1 RNAi. Xenograft and zebrafish model showed that RPTOR blockade suppressed BM of non-small cell lung cancer (NSCLC) and impaired the SPHK2/S1P/STAT3 pathway.
Conclusion: RPTOR is a key driver gene in the brain metastasis of lung cancer, which signifies that RPTORblockade may serve as a promising therapeutic candidate for clinical application.
Genome-Wide Analysis of the Lateral Organ Boundaries Domain (LBD) Gene Family in Sweet Potato
Authors: Shi lei , Lin Xiongjian , Tang Binquan , Zhao Rong , Wang Yichi , Lin Yingyi , Wu Liangliang , Zheng Chao , Zhu Hongbo 
Published: July 2023

Abstract
Background
Sweet potato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. The lateral organ boundary (LOB) structural domain (LBD) genes are a family of genes encoding plant-specific transcription factors that play important roles in regulating plant growth and development, nutrient metabolism, and environmental stresses. However, the function of IbLBD genes in sweet potato remains unclear.
Results
In this study, We identified a total of 53 IbLBD genes in sweet potato. Genetic structure showed that most of the IbLBD genes contained only two exons. Based on the phylogenetic analysis, the IbLBD gene family was classified into class I (45, with the largest number of Ia genes (20)) and class II (8), both classes of proteins contained relatively conservative Motif1 and Motif2 domains. The sweet potato LBD gene was also analyzed regarding its chromosomal distributions, gene duplications, promoters and PPI network. In addition, gene expression profiling and real-time quantitative PCR analysis showed that the expression of 12 IbLBD genes differed in six different tissues and different abiotic stresses. The IbLBD genes of Class I were mainly expressed in the primary root, pencil root, and leaves, while Class II genes were mainly expressed in the different roots of sweet potatoes. Besides the expression pattern analysis of salt and drought treatment plants showed that abiotic stress treatment could significantly induce the expression of IbLBD genes. Specific expression patterns of IbLBD genes under salt and drought stresses suggest their important role in resisting adverse external environments.
Conclusions
Taken together, the molecular basis of LBD genes in sweet potato was analyzed from multiple perspectives, which provides a theoretical basis to further investigate the functions of sweet potato LBD genes.
Predicting Fracture Risk for Chinese Elderly Osteoporosis Patients by Hybrid Model
Authors: Liu Menghan , Wei Xin , Xing Xiaodong , Ma Zicheng , Ren Jiwu , Gao Xiaofeng , Xu Ajing 
Published: July 2023

Abstract
Background: In China, there are many elderly people who suffer from osteoporosis. Fractures, the most serious complication of osteoporosis, can greatly affect people’s quality of life and add pressure to the social pension system. Thus, predicting fracture risk in elderly patients with osteoporosis is imperative, which allows for timely treatment and ameliorates the strain on healthcare resources. Machine learning (ML) models based on Electronic Health Records (EHR) can be contracted to predict fracture risk.
Methods: Data of patients with osteoporosis were extracted from the Electronic Health Records (EHR) in Xinhua Hospital. Demographic and clinical features that are readily available from EHR were used to develop prediction models based on 12 independent ML algorithms (Naïve Bayes classifier [BP], logistic regression [LR], decision tree [DT], k-nearest neighbors [KNN], support vector machine [SVM], random forests [RF], extremely randomized trees [ERT], gradient-boosted decision trees [GBDT], adaptive boosting [AdaBoost], categorical boosting [CatBoost], extreme gradient boosting [XGBoost], multilayer perceptron [MLP]) and 3 hybrid ML models (XGBoost + MLP, XGBoost + LR, XGBoost + SVM). A comprehensive importance score was designed to interpret features from several aspects.
Results: A total of 8530 patients with osteoporosis were included for analysis, of which 1090 cases (12.8%) were fracture patients. The hybrid XGBoost and SVM model had the best predictive performance in terms of accuracy and precision (above 90%) among all models. We obtained 20 important features according to the comprehensive importance score, which represents high-risk factors for fractures and was interpreted from a clinical point of view.
Conclusions: The hybrid XGBoost and SVM model can be reliable tools for predicting the risk of fracture in patients with osteoporosis. And the hybrid model had the best predictive performance, which can be used to assist clinicians in identifying high-risk fracture patients and implementing early interventions.
Cannabis-fruit/vegetable syndrome: an unusual case without pollen co-sensitization
Authors: Wang Joanne , Lam Godfrey , Mak Raymond 
Published: July 2023

Abstract
Background:
Cannabis use has become increasingly popular since its legalization. In 2022, 19% of Canadians over 16 years of age report using cannabis within the past 30 days1. Cannabis is associated with an extensive spectrum of cross-reactivity with fruits and vegetables through a phenomenon known as cannabis-fruit/vegetable syndrome2. While most patients are co-sensitized with cross-reactive pollen, we present a unique case of cannabis-fruit/vegetable syndrome without birch pollen co-sensitization.
Case Presentation:
Since 2021, a 26-year-old female with intermittent cannabis smoking began noticing IgE mediated symptoms when eating previously tolerated fruits within the birch pollen family. Her first instance was with fresh cherries where she instantly experienced ocular/throat pruritus and generalized urticaria. In 2022, she had similar reactions to fresh peaches and raspberries. Concurrently, she began experiencing immediate ocular/throat pruritus with Cannabis sativa but not with Cannabis indica. Her fresh fruit skin test was positive to nectarine (10mm), plum (6mm), raspberry (12mm), blackberry (6mm), and both Cannabis sativa (7mm) and indica (11mm). Her environmental panel was negative to common grass, tree and weed pollens. She was prescribed an epinephrine autoinjector given her systemic symptoms.
Conclusion:
Multiple potential allergens including non-specific lipid transfer proteins (nsLTP), thaumatin-like protein, ribulose-1,5-bisphosphate carboxylase oxygenase, and oxygen evolving enhancer protein are thought to be contributors to cannabis allergies3. Of these, nsLTP is a pan-allergen found ubiquitously throughout the plant kingdom, potentially explaining cross activities between cannabis, fruits, and vegetables. Our case of cannabis-fruit/vegetable syndrome in an otherwise non-atopic individual is interesting as her skin testing showed no reaction against common pollens, specifically birch, a well-known aeroallergen to cross-react with cherries, peaches, and plums. These findings suggest the patient became sensitized to fruits through cannabis use. With increasing cannabis accessibility, more awareness in the medical community is necessary on allergic implications of cannabis use.
Outcomes of allogeneic hematopoietic stem cell transplantation for relapsed or refractory diffuse large B-cell lymphoma
Authors: Kato Koji , Sugio Takeshi , Ikeda Takashi , Yoshitsugu Kanako , Miyzaki Kana , Suzumiya Junji , Yamamoto Go , Kim Sung-Won , Ikegame Kazuhiro , Uehara Yasufumi , Mori Yasuo , Ishikawa Jun , Hiramoto Nobuhiro , Eto Tetsuya , Nakazawa Hideyuki , Kobayashi Hikaru , Serizawa Kentaro , Onizuka Makoto , Fukuda Takahiro , Atsuta Yoshiko , Suzuki Ritsuro 
Published: July 2023

Abstract
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) are curative treatment modalities for diffuse large B-cell lymphoma (DLBCL) because of the intrinsic graft-versus-lymphoma effect. However, limited information is available regarding which patients with relapsed or refractory DLBCL are likely to benefit from allo-HSCT. We retrospectively analyzed data from 1,268 DLBCL patients who received allo-HSCT. The overall survival and progression-free survival (PFS) rates were 30.3% and 21.6% at 3 years, respectively. Multivariate analysis revealed that stable or progressive disease at transplantation, male patient, poorer performance status at transplantation and shorter interval from previous transplantation were associated independently with a lower PFS. Four prognostic factors were used to construct a prognostic index for PFS, predicting 3-year PFS of 55.4%, 43.7%, 20.4% and 6.6%, respectively. The prognostic model predicted relapse rates following allo-HSCT accordingly (P < 0.0001), whereas did not predict transplantation-related mortality (P = 0.249). The prognostic index can identify a subgroup of DLBCL patients whobenefit from allo-HSCT and it is worthwhile to evaluate whether this model is also applicable to patients undergoing allo-HSCT in cases of relapse after chimeric antigen receptor engineered T-cell therapy, although the application of allo-HSCT has been declining with the increase of novel immunotherapies.
Separablilty of metric measure spaces and choice axioms
Authors: Howard Paul 
Published: July 2023

Abstract
We prove that the assertion “For every metric space (X, d ) with a Borel measure μ such that the measure of every open ball is positive and finite, (X, d ) is separable.” lies strictly between the axiom of choice for countable collections of finite sets and the axiom of choice for countable collections of sets in deductive strength in set theory without the axiom of choice. This gives an answer to a question of Dybowski and Gorka [1].