bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efikasi Herbisida Parakuat Diklorida Terhadap Gulma di Perkebunan Kopi Tanaman Belum Menghasilkan
Authors: Sari Resti , Pujisiswanto Hidayat 
Published: January 2025

Testing of the control power of the paraquat dichloride herbicide in suppressing weed growth in immature coffee cultivation was carried out in Sumber Jaya, West Lampung in May-August 2023. The research aimed to obtain an effective dose of paraquat dichloride and determine its phytotoxicity to coffee plants. The treatments consisted of 5 levels of paraquat dichloride dosage, namely 276 g ha-1(B1); 414 g ha-1(B2); 552 g ha-1(B3); 690 g ha-1(B4); and 828 g ha-1(B5) and compared with manual weeding treatment (B6) and control (no treatment) (B7). The research used a Randomized Block Design (RBD) with 4 repetitions. Based on the research results, it can be seen that paraquat dichloride at a dose of 552 - 828 g ha-1 was effective in suppressing the total weed growth of the dominant weeds Asystasia gangetica, Ageratum conyzoides, Synedrella nodiflora, Ottochloa nodosa, Paspalum conjugatum, and Richardia brasiliensis until 8 days after application (DAA). Application of paraquat dichloride at a dose of 414 - 828 g ha-1 was able to control the growth of A. conyzoides and S. nodiflora weeds until 8 DAA. Application of paraquat dichloride at a dose of 276 - 828 g ha-1 on immature coffee plants did not cause symptoms of poisoning.
WITHDRAWN: Optimal Planning of Smart Parking Lot based Combined Hydrogen, Heat and Power (SPL-CHHP) to improve Eco-Techno-Emission Constraints
Authors: Mohammad Suleiman Ibrahim , Oraini Badrea Al , Alkhayyat Ahmad , Vasudevan Asokan , Vaghela Krunal , Dey Protyay , Aggarwal Ankita , Sri G. , Jaafar Mahdi Sh. , Al-KHAFAJI MUSTAFA 
Published: January 2025

Abstract

14 January, 2025. Research Square has withdrawn this preprint after confirming substantial overlap with a manuscript previously submitted by another author.
Exploration of Refined Humane Endpoints for Melioidosis in BALB/c Mice
Authors: Maishman Thomas , Harris Michael , Barnes Kay , Laws Thomas , May Emily , Nelson Michelle , Harding Sarah 
Published: January 2025

Abstract

The development of humane endpoints is critical for refining scientific studies involving animals. Body weight and clinical signs of disease data collected in four recent studies assessing medical countermeasures for utility against the disease melioidosis in mice, was further analyzed. This work used this information to ascertain if a suitable alternative humane endpoint could be identified. A total of 66 possible alternative humane endpoints were explored, which varied the threshold values of the ‘percentage body weight loss post-challenge’ and ‘the clinical signs over time’, following cessation of treatment. The findings indicated a suitable alternative endpoint of a percentage weight loss threshold of 25%, and/or using an average total clinical signs score of ≥ 5 over a 48 hour period. This resulted in a sizeable reduction in median ‘sign-days’ (total clinical score multiplied by the number of days remaining in study) saved per mouse of 21 days, whilst maintaining 100% sensitivity and 93% specificity. In addition, the risk in altering the scientific outcome of each study remained low when utilizing this new endpoint. In conclusion, current humane endpoints in this setting can be refined without negatively impacting the key study findings.
Extracellular Vesicles of Minimalistic Mollicutes as Mediators of Immune Modulation and Horizontal Gene Transfer
Authors: WAGNER THERESA , Torres-Puig Sergi , Yimthin Thatcha , Démoulins Thomas , Jores Jörg 
Published: January 2025

Abstract

Extracellular vesicles (EVs) are central components of bacterial secretomes, including the small, cell wall-less Mollicutes. Although EV release in Mollicutes has been reported, EV proteomic composition and function have not been explored yet.
We developed a protocol for isolating EVs of the pathogens Mycoplasma mycoidessubsp. capri (Mmc) and Mycoplasma (Mycoplasmopsis)bovis and examined their functionality. Proteomic analysis demonstrated that EVs mirror the proteome of their homologous strain. EVs exhibited nuclease activity, effectively digesting both circular and linear DNA. Notably, EVs elicited immune responses in bovine primary blood cells, like those induced by live M. bovis.
Our findings reveal that EVs can carry plasmids and enable their horizontal transfer, known as vesiduction. Specifically, the natural plasmid pKMK1, with an unknown transmission route, was detected in EVs of Mmc 152/93 and the tetM-containing pIV08 plasmid was associated with EVs released by an Mmc GM12 strain carrying this plasmid. pIVB08 could be transferred via homo- and heterologous vesiduction to Mmc, M. capricolum subsp. capricolum and M. leachii. Vesiduction was impeded by membrane disruption but resisted DNase and Proteinase K treatment, suggesting that EVs protect their cargo.
These findings enhance our understanding of Mollicutes EVs, particularly in host interactions and horizontal gene transfer.
Polaritons in non-fullerene acceptors for high responsivity angle-independent organic narrowband infrared photodiodes
Authors: Daskalakis Konstantinos , Abdelmagid Ahmed Gaber , Qiao Zhuoran , Coenegracht Boudewijn , Yu Gaon , Qureshi Hassan Ali , Anthopoulos Thomas , Gasparini Nicola 
Published: January 2025

Abstract

Narrowband infrared organic photodetectors are in great demand for sensing, imaging, and spectroscopy applications. However, most existing strategies for narrowband detection depend on spectral filtering either through saturable absorption, which requires active layers exceeding 500 nm, restricting the choice of materials for producing high-quality films, or cavity effects, which inherently introduce strong angular dispersion. Microcavity exciton-polariton (polariton) modes, which emerge from strong exciton-photon coupling, have recently been explored as an angular dispersion suppression strategy for organic optoelectronics. In this work, we present the first narrowband infrared polariton organic photodiode that combines angle-independent response with a record-high responsivity of 0.24 A/W at 965 nm and -2 V. This device, featuring a 100-nm-thin active layer comprising a non-fullerene acceptor, exhibits a detection mode with a full-width at half-maximum of less than 30 nm and a marginal angular dispersion of under 15 nm across ±45◦. This study highlights the potential of polaritons as an innovative platform for developing next-generation optoelectronic devices that achieve simultaneous enhancements in optical and electronic performance.
Molecular epidemiology of Ascaris lumbricoides following multiple rounds of community-wide treatment: who infects whom?
Authors: Landeryou Toby , Maddren Rosie , Hearn Jack , Belachew Mahlet , -Gomez Santiago Rayment , Liyew Ewnetu Firdawek , Forbes Kathryn , Abtew Birhan , Lawton Scott , Eze Jude , Tasew Geremew , Angulo Ufaysa , Anderson Roy 
Published: January 2025

Abstract

Control and elimination of the parasite Ascaris lumbricoides relies on mass administration using a limited number of anti-helminthics. Whilst these programs have reduced the infection intensity and prevalence within many endemic regions transmission is poorly understood, with reinfection commonly occurring following cessation of treatment. Here, we utilise genomic data to understand parasite transmission within and between households in a community and the genomic impact of repeated MDA. We sequenced 54 whole-genomes from individuals in a longitudinal cohort epidemiological study of transmission and drug treatment extending over 6 years. We found that fine-scale population structure exists in spatially distinct clusters of infected individuals with reinfection occurring within or between geographically close households. This observation helps inform the policy of future control in low prevalence settings suggesting more targeted treatment of infection hotspots We found evidence of positive selection acting on members of gene families previously implicated in reduced drug efficacy but detect no high frequency of impactful variants. As efforts to eliminate A. lumbricoides intensify, our study provides a foundation for genomic surveillance to help identify both who infects whom and the impact of repeated drug treatment.
Molecular Engineering of Melanin for Enhanced Biological γ-ray Protection
Authors: Cao Wei , Deng Ruotong , Li Yuxi , Ou Yining , Wang Jian , Zhang Xuanying , Zhang Junbo , Ruan Qing , Xu Huaping , Chen Wei , Zhang Chunlei , Zhu Hanjie , Zhang Yongxian , Niu Yantao , Hu Zhubin 
Published: January 2025

Abstract

The escalating utilization of ionizing radiation across medicine and industry, coupled with the relentless global nuclear rivalry, underscoring the paramount urgency of effective radioprotective materials. Conventional materials such as lead and concrete are widely used, and lead-free materials have also emerged to solve the problems of cumbersome and toxic lead, such as metal-containing micro/nano materials and polymers. Nevertheless, there is still a significant challenge in meeting the urgent need for lightweight and biocompatible alternatives. To tackle this challenge, this work utilizes molecular engineering of melanin to develop a panel of novel metal-free melanin materials with enhanced conjugation, heightened physical shielding against radiation and effective antioxidant properties. Remarkably, engineered melanin materials demonstrated unprecedented in vivo γ-ray protection, increasing mice survival from 0–100%.
Land Use Land Cover Change Detection in Asabla Watershed, Northern Highlands of Ethiopia
Authors: Amare Abebe , Alemayehu Zewde , Miju Cherinet 
Published: January 2025

Abstract

Purpose
Land use change is a fundamental variable that impacts and links many parts of the human and physical environments. The analysis and monitoring of changes in land use and resources using Landsat imagery is important to understand the extent and magnitude of the changes. Therefore, the study was conducted to analyze the land use land cover change in Asabla watershed Northwest Ethiopia during 2020/22.
Methods
This study examines land use/land cover changes from 1986 to 2020 using Landsat satellite images and field data from (DEM) from USGS using supervised methods in ERDAS Imagine 2010 and ArcGIS 10.3. LU/LC classes, including forestland, grazing land, cultivated land, and settlements, were analyzed. Accuracy assessments were performed using 150 ground control points, with measures like producer and user accuracy, overall accuracy, and Kappa coefficient to evaluate classification precision. The percent change and annual rate of change to assess the magnitude of LU/LC over time was calculated.
Result
The (LU/LC) changes in a watershed from 1986 to 2020, revealing significant transformations across four primary classes: cultivated land, settlements, grazing land, and forestland. Accuracy assessments for the classified images indicated high reliability in 2020 (88.65%) with Kappa values 0.80, signaling strong agreement with ground truth data. Cultivated land increased steadily from 1986 to 2020, primarily at the expense of grazing lands and forest cover. In contrast, forestland showed a consistent increase, largely due to the expansion of eucalyptus plantations.
Conclusion
The study highlights significant LU/LCC in the watershed from 1986 to 2020. These changes are primarily driven by population growth, agricultural expansion, and the establishment of eucalyptus plantations. Therefore, sustainable land management strategies and policy, balancing agricultural expansion with the preservation of grazing lands and agroforestry practices to mitigate the land use change on the environment and local communities could be implemented.
Music and massage for patients undergoing chemotherapy infusion
Authors: Correia Ana Carolina Barbosa dos Santos , Ferreia Alida Rosária Silva , Correia Maria Isabel Toulson Davisson 
Published: January 2025

Abstract

Purpose: It has been found that the combination of music and therapeutic massage has positive effects on cancer patients undergoing chemotherapy infusion, therefore, this study evaluates the effects of music and therapeutic massage on pain, quality of life, and well-being of patients undergoing chemotherapy infusion.
Method: A randomized, crossover study was conducted with patients over 18 years old with cancer, who were randomly allocated to either receive twenty minutes of therapeutic massage and music by a trained physical therapist in the first three chemotherapy sessions or in the last three. Patients were evaluated using the Visual Analog Scale (VAS) for pain, European Organization for Research and Treatment of Cancer Core Quality of Life Questionnaire-30 for quality of life and the Subjective Well-Being Scale for well-being (EBES).
Results: Participants (N=54) had better health-related quality of life (p=0.000* and p=0.002*) and improved well-being about life satisfaction (p=0.008* and p=0.000*) at the end of the study intervention. Patients who started the research receiving the intervention had a better index of positive affect and a decrease in fears and anxieties regarding the upcoming oncology treatment (p=0.001*). There were no differences in the reported pain between the groups. Conclusion: The association of music and therapeutic massage promoted an increase in the quality of life related to health and well-being in cancer patients undergoing chemotherapy treatment.
The Potential Role of BCL2A1⁺ Tissue-Resident Macrophages in the Prognosis of Wilms Tumor
Authors: Wang Wei , Tang Zhishen , Zhu Zhihui , Zhuo Yingquan , Du Jun , Zhu Yuxian , Luo Xi , Xiao Jingjing , Gu Huajian 
Published: January 2025

Abstract

Tumor-associated macrophages (TAMs) have a significant impact on the prognosis and treatment outcomes of Wilms tumor (WT) patients. To explore the key mechanisms underlying WT progression and immune therapy, this study used CIBERSORT to analyze the immune cell infiltration of 120 WT patients. Combined with single-cell RNA sequencing (scRNA-seq) data, the heterogeneity of macrophages in WT and adjacent tissues was revealed, identifying a subpopulation of tissue-resident macrophages with specific expression of BCL2A1. Further validation through immunohistochemistry (IHC) and immunofluorescence (IF) experiments confirmed the presence of BCL2A1⁺ tissue-resident macrophages and showed that their high expression is associated with advanced tumors and poor prognosis. Functional enrichment analysis suggests that BCL2A1⁺ tissue-resident macrophages may promote WT progression through immune regulation and apoptosis pathways. This study is the first to identify the presence of a BCL2A1⁺ tissue-resident macrophage subset in WT and reveal its critical role in tumor progression, potentially providing a novel target for personalized immunotherapy.
Fostering Research Engagement and Ideation: Insights from Medical Students in Thailand
Authors: Plangsiri Settanan , Jirapornsuwan Manisara , Kaewkamjornchai Phanuwich 
Published: January 2025

Abstract

Background
Research plays a crucial role in fostering critical thinking skills in students, which greatly contributes to their future careers. In addition, many life-saving drugs have been developed from discoveries made through medical student research. However, students often struggle to find captivating research topics and suitable advisors. This study aims to facilitate medical student engagement in research by identifying how experienced researchers generate ideas and connect with research advisors.
Methods
A sequential mixed-methods approach was employed, incorporating both quantitative and qualitative surveys. The study focused on the 2023 cohort of third-year medical students, who were required to complete a research proposal as part of their graduation requirements. Quantitative data were analyzed using t-tests and Chi-square tests to assess associations. Additionally, structured interviews were conducted to provide deeper qualitative insights.
Results
The most important factor in initiating research ideas was discussing with faculty staff specializing in the area of interest (91.5%), followed by self-literature review (53.2%), and attending research seminars (46.8%). For successive research projects, attending research seminars became less important (21.9%). Research advisors were primarily found through research seminars (38.1%) and by directly asking class lecturers (20.3%). First research advisors were often class professors (23.4%), while successive research advisors were obtained through other professors' recommendations (28.1%). The interview delved deeper into each participant’s motivations for pursuing research, uncovering their perspectives on how effectively the curriculum facilitated professor matching and their personal experiences with research advisors. Additionally, while many participants expressed a personal interest in research, the majority opposed the idea of making research a mandatory requirement in medical school.
Conclusion
Discussion with faculty staff, personal literature review, and research seminars are crucial in sparking research ideas among medical students. However, research seminars become less relevant for generating ideas after students complete their first research project. Research seminars still play a significant role in helping students find research advisors. Medical schools aiming to promote research among students should support these activities.
Investigation of the factors that cause respiratory tract infection during the COVID-19 pandemic in Afyonkarahisar, Turkey (2020-2021).
Authors: Köklu Ramazan , ŞEN Emek TÜRKEKUL , ESEN Berrin , KONYA Petek ŞARLAK , KORKMAZ Derya , BÜKÜLMEZ Ayşegül , Tahta Evrim Gürhan 
Published: January 2025

Abstract

Background
Respiratory tract infections are a significant cause of morbidity and mortality worldwide. In particular, closely monitoring pathogens with pandemic potential is essential for early diagnosis and control of possible pandemics. In this study, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and viruses that most commonly cause respiratory tract infections in patients with similar clinical symptoms during the COVID-19 pandemic were examined; Influenza A/Influenza B; influenza A H1/H3/H5/H7; Human MetapneumoVirus (HMPV), Human respiratory syncytial virus (HRSV) A/B, Human rhinovirus (HRV); human bocavirus (HBoV); The simultaneous presence of Human enterovirus (HEV), Human parechvirus (HPeV), Human adenovirus (AV), Human coronavirus (HCoV) HKU/NL63/OC43/229E and Parainfluenza (PIV) 1/2/3/4 viruses were investigated.
Methods
Nasopharyngeal swab samples taken from 100 patients were analyzed using the multiplex single-step reverse transcription polymerase chain reaction (Multiplex RT qPCR) method.
Results
SARS-CoV-2 was detected in two (1%) of 50 samples taken from pediatric patients, and Human coronavirus (HCoV) OC43 was positive in one. While 14 samples (28%) were found to be positive for SARS-CoV-2 in the adult patient group, other respiratory tract viruses were not detected.
Conclusions
Although the study was carried out during the high season of influenza and other respiratory viral pathogens, they were found to be at low numbers and ratios. The reason for the low detection rate of SARS-CoV-2 virus and other respiratory tract viruses was interpreted as taking personal precautions and quarantine measures during the COVID-19 pandemic. It was thought that SARS-CoV-2 activity and its effect on seasonal influenza and other respiratory tract virus activities should be followed closely in the coming years.
The Effects of Balance Training on Physical Fitness and Skill- related Performance in Basketball Players: A Systematic Review
Authors: Cao Shudian , Gao Lei , Geok Soh Kim , Liu Jia , Wang Zhaoran 
Published: January 2025

Abstract

Background
Existing evidence suggests that balance training (BT) has a positive impact on physical fitness and sports performance. However, its specific effects on basketball players have not been extensively studied. Therefore, this systematic review aims to analyze the influence of BT on the physical fitness and skill-related performance of basketball players.
Method
A thorough search was conducted across four databases (Web of Science, Scopus, PubMed, and EBSCOhost) for studies published until August 24, 2024, using keywords related to BT and basketball. The quality of the included studies was assessed using the "Qualsyst" tool.
Results
Thirteen studies involving 373 participants were included, focusing on BT interventions lasting a minimum of four weeks. These studies demonstrated that BT led to significant enhancements in balance, power, agility, stability, and basketball skill-related performance. Nevertheless, certain assessments such as the balance error scoring system, triple hop distance, 30-second maximal performance jump, single-leg triple hop, Y reactive agility, and maneuver running tests did not show significant improvements.
Conclusion
BT proves to be a valuable intervention for improving physical fitness and skill-related performance among basketball players. Coaches and trainers should customize training protocols based on player experience and emphasize adequate training volume (totalling 8–24 hours) to maximize outcomes. Future research should delve into gender-specific adaptations and explore the long-term impacts of BT.
Structural Modifications and Prospects of Histone Deacetylase (HDAC) Inhibitors in Cancer
Authors: Chen Yu , Su Jiahong , Li Sha , Chen Feier , Zhang Yan , Wang Xingyue , Zhang Yinping , Wang Xiang , Yuan Zijun , Ren Siqi , He Xinyu , Zhao Yueshui , Wu Xu , Li Mingxing , Du Fukuan , Deng Shuai , Shen Jing , xiao Zhangang 
Published: January 2025

Abstract:
Histone deacetylases (HDACs) play a crucial role in the regulation of cancer
progression and have emerged as key targets for antitumor therapy. Histone Deacetylase
Inhibitors (HDACis) effectively suppress tumor cell proliferation, induce apoptosis, and
cause cell cycle arrest, demonstrating broad-spectrum antitumor activity. This article primarily
focuses on enhancing the selectivity of HDACis through structural modification
using natural compounds. It provides detailed insights into the structure modification of
histone deacetylase 8 (HDAC8) and histone deacetylase 10 (HDAC10), as well as dual--
target inhibitors and their pharmacological effects. Furthermore, conventional HDAC inhibitors
are susceptible to off-target effects and the development of drug resistance. Our
research focuses on augmenting the targeting specificity of HDAC inhibitors through
their combination with proteolysis targeting chimera (PROTAC). Lastly, the latest advancements
in clinical research on HDAC inhibitors were summarized, revealing that these
inhibitors possess limitations in their clinical applications due to intrinsic or acquired
resistance. Consequently, this article primarily focuses on summarizing the current
status and prospects of structural modifications for HDAC inhibitors, with the aim
of inspiring researchers to develop novel HDAC inhibitors exhibiting enhanced activity
for improved application in clinical research.