bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Synthetic Modulator Enabling Long-Term Propagation and Neurogenesis of Human-Derived Neural Progenitor Cells
Authors: Liao Ceheng , Guan Ying , Zheng Jihui , Wang Xue , Wang Meixia , Zhu Zhouhai , Peng Qiyuan , Wang Hong-Hui , Li Meng 
Published: May 2023

AbstractNeural progenitor cells (NPCs) are important cells for in vitro drug screening and the cell-based therapy for brain-related disorders, which requires well-defined and reproducible culture systems. Current strategy the use of protein growth factors presents challenges in terms of reproducibility and cost. In this study, we have developed a novel DNA-based modulator to regulate FGFR signaling of NPCs, enabling maintenance of the stemness over 50 passages and neurogenesis towards neurons. The DNA-based FGFR-agonist effectively promoted FGFR1 phosphorylation and activated the downstream ERK signaling pathway in FGFR1-positive cells. Using human embryonic stem cell lines, we differentiated them into NPCs and replaced basic fibroblast growth factor (bFGF) in the regulator culture medium with DNA-based FGFR-agonist for artificially elicited FGFR signaling. The results demonstrated that the FGFR-agonist could promote NPCs proliferation and neurosphere formation, recapitulating the function of bFGF. Notably, transcriptomic analysis revealed that FGFR-agonist could customize the stemness-associated transcription program, while decouples the neuronal differentiation program, highly resembling that the native ligand, bFGF. Moreover, our culture condition facilitated the successful propagation of NPCs for over 50 passages, while retaining their ability to efficiently differentiate into neurons. Overall, our approach provides a highly effective method for expanding NPCs, offering new opportunities for disease-in-dish research and drug screening for neural degeneration.
UBL3 Interacts with Alpha-synuclein in Cells and the Interaction is Downregulated by the EGFR Pathway Inhibitor Osimertinib
Authors: Chen Bin , Hasan Md. Mahmudul , Zhang Hengsen , Zhai Qing , Waliullah A.S.M. , Ping Yashuang , Zhang Chi , Oyama Soho , Mimi Mst. Afsana , Tomochika Yuna , Nagashima Yu , Nakamura Tomohiko , Kahyo Tomoaki , Ogawa Kenji , Kaneda Daita , Yoshida Minoru , Setou Mitsutoshi 
Published: May 2023

AbstractUbiquitin-like 3 (UBL3) acts as a post-translational modification (PTM) factor and regulates protein sorting into small extracellular vesicles (sEVs). sEVs have been reported as vectors for the pathology propagation of neurodegenerative diseases, such as α-synucleinopathies. Alpha-synuclein (α-syn) has been widely studied for its involvement in α-synucleinopathies. However, it is still unknown whether UBL3 interacts with α-syn, and is influenced by drugs or compounds. In this study, we investigated the interaction between UBL3 and α-syn, and any ensuing possible functional and pathological implications. We found that UBL3 can interact with α-syn by theGaussia princepsbased split luciferase complementation assay in cells and immunoprecipitation, while cysteine residues at its C-terminal, which are considered important as PTM factors for UBL3, were not essential for the interaction. The interaction was upregulated by 1-methyl-4-phenylpyridinium exposure. In drug screen results, the interaction was significantly downregulated by the treatment of osimertinib. These results suggest that UBL3 interacts with α-syn in cells and be significantly downregulated by epidermal growth factor receptor (EGFR) pathway inhibitor osimertinib. Therefore, the UBL3 pathway may be a new therapeutic target for α-synucleinopathies in the future.
Spatial subdomains in the Optic Tectum for the encoding of visual information
Authors: Shallcross Thomas , Diana Giovanni , Burrone Juan , Meyer Martin 
Published: May 2023

1AbstractNeurons across the visual system provide estimates of the visual features they encode. However, the reliability of those estimates can vary across the neuronal population. Here, we use information theory to provide a spatial map of how well neurons can distinguish ethologically-relevant visual stimuli across the entire larval zebrafish optic tectum, a brain region responsible for driving visually guided behaviour. We find that the ability of neurons to discriminate between stimuli is non-uniformly distributed across the tectum. Specifically, we show that information about local motion is preferentially encoded in the posterior tectum, whilst information about whole-field motion is preferentially encoded in the anterior tectum. This is achieved through two systematic changes along the anterior-posterior axis of the tectum: (i) a change in the number of neurons that discriminate between stimuli and (ii) a change in how well each neuron can discriminate between stimuli. By classifying neurons into distinct subtypes based on their response properties we uncovered a small group of neurons that are spatially localised to specific regions of the tectum and are able to discriminate between visual stimuli in a highly reliable manner. Our results highlight the importance of implementing information theoretic approaches to assess visual responses and provide a novel description of regional specialisation in the zebrafish optic tectum.
Cellular Bioenergetics Effects of Sirolimus on the Gonads of BALB/c Mice
Authors: Narchi Hassib , Al Bawardi Alia , Thachillath Pramathan , Souid Abdul-Kader 
Published: May 2023

AbstractBackgroundSirolimus, an immunosuppressive drug widely used in organ transplantation, inhibits the mechanistic target of rapamycin (mTOR), decreases cellular respiration, stops cell cycle progression and causes apoptosis. It has been linked to cytotoxicity in the gonads, but the cause is unknown.AimsTo understand the effects of sirolimus on the gonads by measuring cellular respiration and ATP as indicators of cellular damage, and examining histological changes.Material and MethodsWe used 6-8 week old male and female BALB/c mice. They received 5 µg/g sirolimus via intraperitoneal injections for 5 consecutive days per week for up to 3 weeks (treated group) or DMSO (control group). Upon euthanasia, the gonads were promptly removed while the heart was still beating, weighed, and processed for cellular respiration, ATP measurement, and histological studies. Cellular respiration was measured using a phosphorescence oxygen analyzer and ATP using a bioluminescent assay system. Histology was performed using processed tissue fragments stained with hematoxylin and eosin (H&E).ResultsCellular ATP levels in testicular tissue were higher than ovarian tissue in both groups, but this was not significant (p=0.4 and 0.2). Although the sirolimus group had higher cellular ATP levels, no significant difference was observed in either testicular (p=0.6) or ovarian (p=0.9) tissue. Both DMSO (p=0.01) and sirolimus groups (p=0.008) showed lower cellular respiration in ovarian than testicular tissue. Cellular respiration was lower in sirolimus group than DMSO group in both testicular (p=0.6) and ovarian (p=0.2) tissue. Testicular cellular respiration remained unchanged up to day 11 before declining, while ovarian respiration reduced up to day 11 and remained unchanged. Testicular histopathology showed normal sperm production and normal follicular development in ovarian tissue.ConclusionThe results suggest that the 5 µg/g dose of sirolimus does not cause significant short-term changes in the cellular bioenergetics of the gonads or produce noticeable histopathological changes.
Dysfunctional Mitochondria in Cardiac Fibers of a Williams-Beuren Syndrome Mouse Model
Authors: Abdalla Noura , Tobías-Baraja Ester , Gonzalez Alejandro , Garrabou Gloria , Egea Gustavo , Campuzano Victoria 
Published: May 2023

SummaryWilliams-Beuren Syndrome (WBS) is a rare neurodevelopmental disorder that, together with a rather characteristic neurocognitive profile, presents a strong cardiovascular phenotype. The cardiovascular features of WBS are mainly related to a gene dosage effect due to hemizygosity of the elastin (ELN) gene; however, the phenotypic variability between WBS patients indicates the presence of important modulators of the clinical impact of elastin deficiency. Recently, two genes within the WBS region have been linked to mitochondrial dysfunction. Numerous cardiovascular diseases are related to mitochondrial dysfunction; therefore, it could be a modulator of the phenotype present in WBS. Here, we analyze mitochondrial function and dynamics in cardiac tissue from a WBS complete deletion (CD) model. Our research reveals that cardiac fiber mitochondria from CD animals have altered mitochondrial dynamics, accompanied by respiratory chain dysfunction with decreased ATP production, reproducing alterations observed in fibroblasts from WBS patients. Our results highlight two major factors; on the one hand, that mitochondrial dysfunction is probably a relevant mechanism underlying several risk factors associated with WBS disease; on the other, the CD murine model mimics the mitochondrial phenotype of WBS and could be a great model for carrying out preclinical tests on drugs targeting the mitochondria.
Optogenetic spatial patterning of cooperation in yeast populations
Authors: Le Bec Matthias , Pouzet Sylvain , Cordier Céline , Barral Simon , Scolari Vittore , Sorre Benoit , Banderas Alvaro , Hersen Pascal 
Published: May 2023

AbstractMicrobial communities are a siege of complex metabolic interactions such as cooperation and competition for resources. Methods to control such interactions could lead to major advances in our ability to engineer microbial consortia for bioproduction and synthetic biology applications. Here, we used optogenetics to control invertase production in yeast, thereby creating landscapes of cooperator and cheater cells. Yeast cells behave as cooperators (i.e.,transform sucrose into glucose, a public “good”) upon blue light illumination or cheaters (i.e.,consume glucose produced by cooperators to grow) in the dark. We show that cooperators benefit best from the hexoses they produce when their domain size is constrained between two cut-off length-scales. From an engineering point of view, the system behaves as a band pass filter. The lower limit is the trace of cheaters’ competition for hexoses, while the upper limit is defined by cooperators’ competition for sucrose. Hence, cooperation mostly occurs at the frontiers with cheater cells, which not only compete for hexoses but also cooperate passively by letting sucrose reach cooperators. We anticipate that this optogenetic method could be applied to shape metabolic interactions in a variety of microbial ecosystems.
Dual control of host actin polymerization by aLegionellaeffector pair
Authors: Pillon M. , Michard C. , Baïlo N. , Bougnon J. , Picq K. , Dubois O. , Andrea C. , Attaiech L. , Daubin V. , Jarraud S. , Kay E. , Doublet P. 
Published: May 2023

ABSTRACTHost actin cytoskeleton is often targeted by pathogenic bacteria through the secretion of effectors.Legionella pneumophilavirulence relies on the injection of the largest known arsenal of bacterial proteins, over 300 Dot/Icm Type 4 Secretion System effectors, into the host cytosol. Here we define the functional interactions between VipA and LegK2, two effectors with antagonistic activities towards actin polymerization that have been proposed to interfere with the endosomal pathway. We confirmed the prominent role of LegK2 effector inLegionellainfection, as the deletion oflegK2results in defects in the inhibition of actin polymerization at theLegionellaContaining Vacuole, as well as in endosomal escape of bacteria and subsequent intracellular replication. More importantly, we observed the restoration of theΔlegK2mutant defects, upon deletion ofvipAgene, making LegK2/VipA the first example of effector-effector suppression pair that targets the actin cytoskeleton and whose functional interaction impactsL. pneumophilavirulence. We demonstrated that LegK2 and VipA do not modulate each other’s activity in a ‘metaeffector’ relationship. Instead, the antagonistic activities of the LegK2/VipA effector pair would target different substrates, Arp2/3 for LegK2 and G-actin for VipA, to temporally control actin polymerization at the LCV and interfere with phagosome maturation and endosome recycling, thus contributing to the intracellular life cycle of the bacterium. Strikingly, the functional interaction between LegK2 and VipA is consolidated by an evolutionary history that has refined the best effector repertoire for the benefit ofL. pneumophilavirulence.
Paradoxical relationship between esports toxicity and toxicity tolerance: moderated mediation by gender and positive reappraisal coping
Authors: Chang Kun , Uhm Jun-Phil , Kim Sanghoon , Lee Hyun-Woo 
Published: May 2023

PurposeDespite the negative effects of toxicity on various aspects of esports communities, gamers continue to enthusiastically show their pride and engage with the game. Based on the stress and coping theory, the current study aims to shed light on how esports gamers cope with toxicity to develop toxicity tolerance by the mediation effect of positive reappraisal coping strategy.Design/methodology/approachA total of 456 gamers were included in the analysis. Structural equation modeling was performed to evaluate the hypothesized model. Gender differences in the toxicity-coping process were investigated using multi-group analysis.FindingsThe findings revealed the full mediation effect of positive reappraisal on the relationship between toxicity and toxicity tolerance, especially for male gamers. The empirical evidence of this study contributes to theorizing the transformative role of positive reappraisal in developing positive consumption outcomes when esports gamers experience toxicity in the game. The multi-group analysis provided further insights into differentiating the applicability and effectiveness of positive reappraisal based on gender.Originality/valueThe findings contributed to sport management and communication literature by allowing researchers and practitioners to move beyond a preventive coping mindset by facilitating a positive coping environment that encourages gamers to interpret the conversation and messages in a more positive manner.
The nonlinear nexus between renewable energy consumption and economic growth in OECD countries: evidence from PTR and PSTR estimates
Authors: Ferhi Afifa , Kamel Helali 
Published: May 2023

PurposeToday, the increasing use of fossil fuels, energy security, concerns and the great importance of achieving sustainable economic growth underscore the urgent need to transition to a green energy system as soon as possible. To shed light on the relationship between the economy and renewable energy, this study assesses the nonlinear relationship between renewable energy consumption and economic growth for 24 OECD countries between 1990 and 2015.Design/methodology/approachThe authors apply two nonlinear models: panel threshold regression (PTR) and panel smooth transition regression (PSTR).FindingsThe results show that the positive effect of renewable energy consumption on economic growth is conditional. On the one hand, the results of the nonlinear PTR model yielded a threshold value for renewable energy consumption of about 251.17. Below this threshold, the authors find a negative impact of renewable energy consumption on economic growth. However, above this threshold, renewable energy consumption becomes a favorable source of economic growth. Using the nonlinear PSTR model based on the gamma transition parameter of 2.014, the transition from low renewable energy consumption regime to higher is abrupt.Originality/valueReferring to previous studies analyzing linear causality between renewable energy and economic growth, most of the results show various mixed and non-stable effects over the study period. The contributions of this study consist in conduct a series of empirical tests of the nonlinear effects of renewable energy use on economic growth using two nonlinear approaches such as the PTR and PSTR models. If the authors show that such a relationship is nonlinear, it is essential to check whether the transition from one weak regime to another strong regime is abrupt or smooth, using the PSTR approach.
Domestic intercultural experiential learning: a case study working with the Afghan refugee population
Authors: Hawes Frances , Jones Christopher 
Published: May 2023

PurposeCivic engagement involves working to make a difference in one’s community. Experiential learning enhances civic knowledge and promotes the process of reflection that students can take beyond their educational experiences. The purpose of this paper is to identify whether civic engagement may be increased through domestic experiential learning experiences and to inform future educators about how a domestic immersion experience can be used to increase civic engagement among students.Design/methodology/approachTwenty-five students who participated in a domestic immersion experience in January 2022 were sampled. Reflection and changes in civic engagement were measured with student responses to questions before, during and after the immersion.FindingsDuring the preimmersion, students showed a desire to learn about the Afghan refugee population and considered preimmersion anxiety mitigation strategies. Using the Association of American Colleges and Universities’ Civic engagement VALUE rubric, the four main themes were diversity of communities and cultures, civic identity and commitment, civic communication and civic action and reflection. The main themes yielded post immersion were increased civic engagement and aptitude to apply knowledge and skills. The subcategories of continued civic engagement were career planning, volunteering and educating others.Originality/valueThe findings provide guidelines to future work in civic engagement. This case study provides insights into the impacts of domestic immersion experiences and how it allows the opportunity for experiential learning.
The impact of dynamic capabilities on circular economy: the mediating effect of the industrial Internet of things
Authors: AL-Khatib Ayman wael 
Published: May 2023

PurposeThe current study aimed to identify the impact of the dynamic capabilities on the circular economy in Jordan's small- and medium-sized manufacturing enterprises sector as and the mediating role of industrial Internet of things.Design/methodology/approachThis study surveyed small and medium manufacturing enterprises operating in Jordan and collected 459 responses through an online self-administered questionnaire. The effects of dynamic capabilities and the industrial Internet of things on the circular economy were analyzed using covariance-based structural equation modeling (CB-SEM).FindingsEmpirical results showed that all hypotheses are accepted. The results also revealed that the relationship between dynamic capabilities (sensing, seizing and reconfiguring) on a circular economy is mediated by the industrial Internet of things.Originality/valueThis work expanded the application of the dynamic capabilities view to investigate the antecedent effects of the industrial Internet of things and the circular economy and deepened understanding of the dynamic capabilities view theory in assessing the dynamic activities of these firms based on the three dimensions of sensing, seizing and reconfiguring. In general, this study made a theoretical and managerial contribution to supply chain and circular economy literature.
Performance of steel beam with welded top-seat angle connections at elevated temperatures
Authors: Rezaeian Abbas , Mansoori Mona , Khajehdezfuly Amin 
Published: May 2023

PurposeTop-seat angle connection is known as one of the usual uncomplicated beam-to-column joints used in steel structures. This article investigates the fire performance of welded top-seat angle connections.Design/methodology/approachA finite element (FE) model, including nonlinear contact interactions, high-temperature properties of steel, and material and geometric nonlinearities was created for accomplishing the fire performance analysis. The FE model was verified by comparing its simulation results with test data. Using the verified model, 24 steel-framed top-seat angle connection assemblies are modeled. Parametric studies were performed employing the verified FE model to study the influence of critical factors on the performance of steel beams and their welded angle joints.FindingsThe results obtained from the parametric studies illustrate that decreasing the gap size and the top angle size and increasing the top angles thickness affect fire behavior of top-seat angle joints and decrease the beam deflection by about 16% at temperatures beyond 570 °C. Also, the fire-resistance rating of the beam with seat angle stiffener increases about 15%, compared to those with and without the web stiffener. The failure of the beam happens when the deflections become more than span/30 at temperatures beyond 576 °C. Results also show that load type, load ratio and axial stiffness levels significantly control the fire performance of the beam with top-seat angle connections in semi-rigid steel frames.Originality/valueDevelopment of design methodologies for these joints and connected beam in fire conditions is delayed by current building codes due to the lack of adequate understanding of fire behavior of steel beams with welded top-seat angle connections.
Research on ERS point registration of large-size measurement field based on neural network non-uniform temperature field
Authors: Huang Lulu , Huang Xiang , Li Shuanggao 
Published: May 2023

Purpose
Large size of aircraft assembly tooling structure and complex measurement environment exist. The laid enhanced reference points (ERS) are subject to a combination of nonuniform temperature fields and measurement errors, resulting in increased measurement registration errors. In view of the nonuniform temperature field and measurement errors affecting the ERS point registration problem, the purpose of this paper is to propose a neural network-based ERS point registration compensation method for large-size measurement fields under a nonuniform temperature field.

Design/methodology/approach
The approach is to collect ERS point information and temperature data, normalize the collected data to complete the data structure design and complete the construction of the neural network prediction model by data training. The data learning is performed to complete the prediction model construction, and the prediction model is used to complete the compensation analysis of ERS points. Finally, the algorithm is verified through experiments and engineering practice.

Findings
Experimental results show that the proposed neural network-based ERS point prediction and compensation method for nonuniform temperature fields effectively predicts ERS point deformation under nonuniform temperature fields compared with the conventional method. After the compensation analysis, the registration error is effectively reduced to improve registration accuracy. Reducing the combined effect of environmental nonuniform temperature field and measurement error has apparent advantages.

Originality/value
The method reduces the registration error caused by combining a nonuniform temperature field and measurement error. It can be used for aircraft assembly site prediction and registration error compensation analysis, which is essential to improve measurement accuracy further.