bluuebunny/crossref_metadata_2025_split huggingface dataset:
Statistical modeling of Si-based refractory compounds of bamboo leaf and alumina reinforced Al–Si–Mg alloy hybrid composites
Authors: Adesina Olanrewaju S. , Adediran Adeolu A. , Edoziuno Francis O. , Sanyaolu Olufemi O. , Obadele Babatunde A. 
Published: April 2023

AbstractWear properties of Al–Mg–Si alloy matrix hybrid composites made with Si-based refractory compounds (SBRC) derived from bamboo leaf ash (BLA) as complimentary reinforcement with alumina have been studied. The experimental result indicate that optimum wear loss was obtained at higher sliding speed. The wear rate of the composites increased with an increase in BLA wt. %, with the composites having 4%SBRC from BLA + 6% alumina (B4) showing the least wear loss for the different sliding speeds and wear loads considered. With increasing BLA weight percent, the composites' wear mechanism was mostly abrasive wear. Numerical optimization results using central composite design (CCD) reveal that at a wear load of 587.014N, sliding speed of 310.053 rpm and B4 hybrid filler composition level respectively, minimum responses in wear rate (0.572mm2/min), specific wear rate (0.212cm2/g.cm3) and wear loss (0.120 g) would be obtained for the developed AA6063 based hybrid composite. Perturbation plots indicate that the sliding speed have more impact on wear loss, while wear load have significant impact on the wear rate and specific wear rate.
A set of SSR markers to characterize genetic diversity in all Viburnum species
Authors: Hamm Trinity P. , Nowicki Marcin , Boggess Sarah L. , Ranney Thomas G. , Trigiano Robert N. 
Published: April 2023

AbstractAbout 160 species are classified within the Viburnum genus and many of these are cultivated for horticultural purposes. The vast dispersal of Viburnum makes the genus a useful model for studying evolutionary history and inferring how species expanded into their current distributions. Simple sequence repeat (SSR) markers were previously developed for five Viburnum species that were classified within the four major clades (Laminotinus, Crenotinus, Valvatotinus, and Porphyrotinus). The ability of some of these markers to cross-amplify in Viburnum species has been scantly evaluated, but there has not been any genus-wide assessment for the markers. We evaluated a collection of 49 SSR markers for the ability to cross-amplify in 224 samples, including 46 Viburnum species, representing all 16 subclades, and five additional species in the Viburnaceae and Caprifoliaceae. A subset of 14 potentially comprehensive markers for Viburnum species was identified and evaluated for the ability to detect polymorphisms in species outside of their respective clades. The 49 markers had overall amplification success in 52% of the samples, including a 60% success rate within the Viburnum genus and 14% in other genera. The comprehensive marker set amplified alleles in 74% of all samples tested, including 85% of Viburnum samples and 19% of outgroup samples. To the best of our knowledge, this is the first comprehensive set of markers able to characterize species across an entire genus. This set of markers can be used to assess the genetic diversity and population structure of most Viburnum species and closely allied species.
Promising photocatalytic and antimicrobial activity of novel capsaicin coated cobalt ferrite nanocatalyst
Authors: El-Khawaga Ahmed M. , Elsayed Mohamed A. , Fahim Yosri A. , Shalaby Rasha E. 
Published: April 2023

AbstractIn this study, CoFe2O4 nanoparticles were prepared by the co-precipitation method then surface modified with Capsaicin (Capsicum annuum ssp.). The virgin CoFe2O4 NPs and Capsaicin-coated CoFe2O4 NPs (CPCF NPs) were characterized by XRD, FTIR, SEM, and TEM. The antimicrobial potential and photocatalytic degradation efficiencies of the prepared samples via Fuchsine basic (FB) were investigated. The results revealed that CoFe2O4 NPs have spherical shapes and their diameter varied from 18.0 to 30.0 nm with an average particle size of 25.0 nm. Antimicrobial activity was tested on Gram-positive (S. aureusATCC 52923) and Gram-negative (E. coli ATCC 52922) by disk diffusion and broth dilution methods to determine the zone of inhibition (ZOI) and minimum inhibitory concentration (MIC), respectively. UV-assisted photocatalytic degradation of FB was examined. Various parameters affecting the photocatalytic efficiency such as pH, initial concentration of FB, and dose of nanocatalyst were studied. The in-vitro ZOI and MIC results verified that CPCF NPs were more active upon Gram-Positive S. aureus ATCC 52923 (23.0 mm ZOI and 0.625 μg/ml MIC) than Gram-Negative E. coli ATCC 52922 (17.0 mm ZOI and 1.250 μg/ml MIC). Results obtained from the photocatalytic activity indicated that the maximum FB removal achieving 94.6% in equilibrium was observed using 20.0 mg of CPCF NPS at pH 9.0. The synthesized CPCF NPs were effective in the removal of FB and also as potent antimicrobial agent against both Gram-positive and Gram-negative bacteria with potential medical and environmental applications.
The structure of the hematopoietic system can explain chronic myeloid leukemia progression
Authors: Pérez-Jiménez Mario , Derényi Imre , Szöllősi Gergely J. 
Published: April 2023

AbstractAlmost all cancer types share the hallmarks of cancer and a similar tumor formation: fueled by stochastic mutations in somatic cells. In case of chronic myeloid leukemia (CML), this evolutionary process can be tracked from an asymptomatic long-lasting chronic phase to a final rapidly evolving blast phase. Somatic evolution in CML occurs in the context of healthy blood production, a hierarchical process of cell division; initiated by stem cells that self-renew and differentiate to produce mature blood cells. Here we introduce a general model of hierarchical cell division explaining the particular progression of CML as resulting from the structure of the hematopoietic system. Driver mutations confer a growth advantage to the cells carrying them, for instance, the BCR::ABL1 gene, which also acts as a marker for CML. We investigated the relation of the BCR::ABL1 mutation strength to the hematopoietic stem cell division rate by employing computer simulations and fitting the model parameters to the reported median duration for the chronic and accelerated phases. Our results demonstrate that driver mutations (additional to the BCR::ABL1 mutation) are necessary to explain CML progression if stem cells divide sufficiently slowly. We observed that the number of mutations accumulated by cells at the more differentiated levels of the hierarchy is not affected by driver mutations present in the stem cells. Our results shed light on somatic evolution in a hierarchical tissue and show that the clinical hallmarks of CML progression result from the structural characteristics of blood production.
The thiolation of uridine 34 in tRNA, which controls protein translation, depends on a [4Fe-4S] cluster in the archaeum Methanococcus maripaludis
Authors: Bimai Ornella , Legrand Pierre , Ravanat Jean-Luc , Touati Nadia , Zhou Jingjing , He Nisha , Lénon Marine , Barras Frédéric , Fontecave Marc , Golinelli-Pimpaneau Béatrice 
Published: April 2023

AbstractThiolation of uridine 34 in the anticodon loop of several tRNAs is conserved in the three domains of life and guarantees fidelity of protein translation. U34-tRNA thiolation is catalyzed by a complex of two proteins in the eukaryotic cytosol (named Ctu1/Ctu2 in humans), but by a single NcsA enzyme in archaea. We report here spectroscopic and biochemical experiments showing that NcsA from Methanococcus maripaludis (MmNcsA) is a dimer that binds a [4Fe-4S] cluster, which is required for catalysis. Moreover, the crystal structure of MmNcsA at 2.8 Å resolution shows that the [4Fe-4S] cluster is coordinated by three conserved cysteines only, in each monomer. Extra electron density on the fourth nonprotein-bonded iron most likely locates the binding site for a hydrogenosulfide ligand, in agreement with the [4Fe-4S] cluster being used to bind and activate the sulfur atom of the sulfur donor. Comparison of the crystal structure of MmNcsA with the AlphaFold model of the human Ctu1/Ctu2 complex shows a very close superposition of the catalytic site residues, including the cysteines that coordinate the [4Fe-4S] cluster in MmNcsA. We thus propose that the same mechanism for U34-tRNA thiolation, mediated by a [4Fe-4S]-dependent enzyme, operates in archaea and eukaryotes.
Exploring the components and mechanisms of Shen-qi-wang-mo granule in the treatment of retinal vein occlusion by UPLC-Triple TOF MS/MS and network pharmacology
Authors: Zhao Yi , Ma Cui , Qiu Qinghua , Huang Xucong , Qiaolongbatu Xijier , Qu Han , Wu Jiaqi , Fan Guorong , Wu Zhenghua 
Published: April 2023

AbstractThis study aimed to explore the substance basis and mechanisms of Shen-qi-wang-mo Granule (SQWMG), a traditional Chinese medicine prescription that had been clinically utilized to treat retinal vein occlusion (RVO) for 38 years. Components in SQWMG were analyzed by UPLC-Triple-TOF/MS and a total of 63 components were identified with ganoderic acids (GA) being the largest proportion. Potential targets of active components were retrieved from SwissTargetPrediction. RVO-related targets were acquired from related disease databases. Core targets of SQWMG against RVO were acquired by overlapping the above targets. The 66 components (including 5 isomers) and 169 targets were obtained and concluded into a component-target network. Together with biological enrichment analysis of targets, it revealed the crucial role of the “PI3K-Akt signaling pathway”, “MAPK signaling pathway” and their downstream factor iNOS and TNF-α. The 20 key targets of SQWMG in treating RVO were acquired from the network and pathway analysis. The effects of SQWMG on targets and pathways were validated by molecular docking based on AutoDock Vina and qPCR experiment. The molecular docking showed great affinity for these components and targets, especially on ganoderic acids (GA) and alisols (AS), which were both triterpenoids and qPCR exhibited remarkably reduced inflammatory factor gene expression through regulation of these two pathways. Finally, the key components were also identified from rat serum after treatment of SQWMG.
Total fat and omega-3 fatty acids intake in relation to serum brain-derived neurotrophic factor (BDNF) levels and psychological disorders in Iranian adults
Authors: Bahadorpour Sedigheh , Hajhashemy Zahra , Mohammadi Sobhan , Mokhtari Elahe , Heidari Zahra , Saneei Parvane 
Published: April 2023

AbstractConsidering contradictory findings of previous investigations and growing prevalence of psychological disorders, we investigated association between dietary total fat and omega-3 fatty acids intake with serum brain-derived neurotrophic factor (BDNF) levels, depression, anxiety and psychological distress in Iranian adults. Using a multistage cluster random sampling method, 533 middle-aged adults were included in this cross-sectional study. A validated semi-quantitative 168-item food frequency questionnaire was used to examine dietary intakes. A 12-h fasting blood sample was drawn to measure serum BDNF. Serum BDNF values in the first decile were considered low level. Hospital Anxiety and Depression Scale (HADS) and General Health Questionnaire (GHQ) were used to assess depression, anxiety and psychological distress. A U-shaped relationship between fat intake and prevalence of anxiety and distress was found. The third quartile of fat intake compared to the first quartile was significantly related to 80% decreased odds of depression (OR = 0.20, 95% CI 0.05–0.80), in fully-adjusted model. Participants in the third quartile of fat intake compared to those in the first quartile had significantly 45% lower odds for distress, in the crude model (OR = 0.55, 95% CI 0.33–0.92); however, this association disappeared after considering confounders. There was no significant association between omega-3 fatty acids intake and odds of depression, anxiety or distress. Prevalence of low-BDNF values was higher in participants with depression, as compared to non-depressed subjects (14.9 vs. 9%; P = 0.06). This cross-sectional study illustrated a U-shaped relationship between fat intake and prevalence of anxiety and distress. Moderate intake of fat was related to lower odds of depression. Prevalence of low-BDNF values was slightly higher in subjects with depression compared to non-depressed individuals.
Intensification of strontium (II) ion biosorption on Sargassum sp via response surface methodology
Authors: Soleymani F. , Khani M. H. , Pahlevanzadeh H. , Amini Younes 
Published: April 2023

AbstractA batch system was employed to investigate the biosorption of strontium (II) on Sargassum sp. The biosorption of strontium on Sargassum sp was studied with response surface methodology to determine the combined effect of temperature, initial metal ion concentration, biomass treatment, biosorbent dosage and pH. Under optimal conditions, the algae's biosorption capacity for strontium (initial pH 7.2, initial strontium concentration 300 mg/l for Mg-treated biomass and biosorbent dosage 0.1 g in 100 mL metal solution) was measured at 103.95 mg/g. In our analysis, equilibrium data were fitted to Langmuir and Freundlich isotherms. Results show that the best fit is provided by the Freundlich model. Biosorption dynamics analysis of the experimental data indicated that strontium (II) was absorbed into algal biomass in accordance with the pseudo-second-order kinetics model well.
HX009, a novel BsAb dual targeting PD1 x CD47, demonstrates potent anti-lymphoma activity in preclinical models
Authors: Ke Hang , Zhang Faming , Wang Jingjing , Xiong Lingxin , An Xiaoyu , Tu Xiaolong , Chen Cen , Wang Yueying , Mao Binchen , Guo Sheng , Ju Cunxiang , He Xiangfei , Sun Ruilin , Zhang Lei , O’Connor Owen A. , Li Qi-Xiang 
Published: April 2023

AbstractBoth PD1/PD-L1 and CD47 blockades have demonstrated limited activity in most subtypes of NHL save NK/T-cell lymphoma. The hemotoxicity with anti-CD47 agents in the clinic has been speculated to account for their limitations. Herein we describe a first-in-class and rationally designed bispecific antibody (BsAb), HX009, targeting PD1 and CD47 but with weakened CD47 binding, which selectively hones the BsAb for tumor microenvironment through PD1 interaction, potentially reducing toxicity. In vitro characterization confirmed: (1) Both receptor binding/ligand blockade, with lowered CD47 affinity; (2) functional PD1/CD47 blockades by reporter assays; (3) T-cell activation in Staphylococcal-enterotoxin-B-pretreated PBMC and mixed-lymphocyte-reaction. In vivo modeling demonstrated antitumor activity in Raji-B and Karpass-229-T xenograft lymphomas. In the humanized mouse syngeneic A20 B-lymphoma (huCD47-A20) HuGEMM model, which has quadruple knocked-in hPD1xhPD-L1xhCD47xhSIRPα genes and an intact autologous immune-system, a contribution of effect is demonstrated for each targeted biologic (HX008 targeting PD1 and SIRPα-Fc targeting CD47), which is clearly augmented by the dual targeting with HX009. Lastly, the expression of the immune-checkpoints PD-L1/L2 and CD47 seemed co-regulated among a panel of lymphoma-derived-xenografts, where HX009 maybe more effective in those with upregulated CD47. Our data warrants HX009’s further clinical development for treating NHLs.
Mortality was predicted by depression and functional dependence in a cohort of elderly adults of Italian descent from southern Brazil
Authors: Pessin Emeline , Fuchs Sandra C. , Bruscato Neide M. , Fuchs Felipe C. , Moriguchi Emilio H. 
Published: April 2023

AbstractThe older population has an increasing burden of non-communicable disease, which can potentially be associated with physical and mental disabilities and shorten life spam. To investigate whether depression, loss of functionality for activities of daily living, and lower social support are associated with all-cause mortality in the older population of Italian descent. This population-based cohort study was conducted in Veranópolis, a country city from southern Brazil, among individuals aged 60 years or older. Interviews were performed in a systematic random sampling regarding demographic, socioeconomic, and psychosocial variables, in addition to depression (Geriatric Depression Scale), activities of daily living (Barthel Index), and social support (Medical Outcomes Study scale). In the follow-up, participants were reinterviewed or, in case of death, the next of kin, and hospital records were revised. Hierarchical analysis was used to determine characteristics independently associated with all-cause mortality, using Poisson regression with robust variance, expressed as relative risk with 95% confidence intervals (RR; 95%CI). A total of 997 participants were enrolled and 882 participants completed the study, after 7.24 ± 2.41 years; with 581 remaining alive. The mean age was 73.12 ± 8.03 years, 4% were nonagenarians or centennials, and 62% were women. Symptoms of depression (RR: 1.04; 1.01–1.06) and functional dependence for ADL (RR: 1.00; 0.99–1.00) were associated with all-cause mortality, even after controlling for confounding factors. Lower social support was not associated with mortality (RR: 1.00; 0.99–1.01). Depression and functional dependence are independent predictors of all-cause mortality in the older population from Italian descent.
Impact of trauma teams on high grade liver injury care: a two-decade propensity score approach study in Taiwan
Authors: Tang Wen-Ruei , Wu Chun-Hsien , Yang Tsung-Han , Yen Yi-Ting , Hung Kuo-Shu , Wang Chih-Jung , Shan Yan-Shen 
Published: April 2023

AbstractHigh-grade liver laceration is a common injury with bleeding as the main cause of death. Timely resuscitation and hemostasis are keys to the successful management. The impact of in-hospital trauma system on the quality of resuscitation and management in patients with traumatic high-grade liver laceration, however, was rarely reported. We retrospectively reviewed the impact of team-based approach on the quality and outcomes of high-grade traumatic liver laceration in our hospital. Patients with traumatic liver laceration between 2002 and 2020 were enrolled in this retrospective study. Inverse probability of treatment weighting (IPTW)-adjusted analysis using the propensity score were performed. Outcomes before the trauma team establishment (PTTE) and after the trauma team establishment (TTE) were compared. A total of 270 patients with liver trauma were included. After IPTW adjustment, interval between emergency department arrival and managements was shortened in the TTE group with a median of 11 min (p < 0.001) and 28 min (p < 0.001) in blood test reports and duration to CT scan, respectively. Duration to hemostatic treatments in the TTE group was also shorter by a median of 94 min in patients receiving embolization (p = 0.012) and 50 min in those undergoing surgery (p = 0.021). The TTE group had longer ICU-free days to day 28 (0.0 vs. 19.0 days, p = 0.010). In our study, trauma team approach had a survival benefit for traumatic high-grade liver injury patients with 65% reduction of risk of death within 72 h (Odds ratio (OR) = 0.35, 95% CI = 0.14–0.86) and 55% reduction of risk of in-hospital mortality (OR = 0.45, 95% CI = 0.23–0.87). A team-based approach might contribute to the survival benefit in patients with traumatic high-grade liver laceration by facilitating patient transfer from outside the hospital, through the diagnostic examination, and to the definitive hemostatic procedures.
PIEZO1 and PECAM1 interact at cell-cell junctions and partner in endothelial force sensing
Authors: Chuntharpursat-Bon Eulashini , Povstyan Oleksandr V. , Ludlow Melanie J. , Carrier David J. , Debant Marjolaine , Shi Jian , Gaunt Hannah J. , Bauer Claudia C. , Curd Alistair , Simon Futers T. , Baxter Paul D. , Peckham Michelle , Muench Stephen P. , Adamson Antony , Humphreys Neil , Tumova Sarka , Bon Robin S. , Cubbon Richard , Lichtenstein Laeticia , Beech David J. 
Published: April 2023

AbstractTwo prominent concepts for the sensing of shear stress by endothelium are the PIEZO1 channel as a mediator of mechanically activated calcium ion entry and the PECAM1 cell adhesion molecule as the apex of a triad with CDH5 and VGFR2. Here, we investigated if there is a relationship. By inserting a non-disruptive tag in native PIEZO1 of mice, we reveal in situ overlap of PIEZO1 with PECAM1. Through reconstitution and high resolution microscopy studies we show that PECAM1 interacts with PIEZO1 and directs it to cell-cell junctions. PECAM1 extracellular N-terminus is critical in this, but a C-terminal intracellular domain linked to shear stress also contributes. CDH5 similarly drives PIEZO1 to junctions but unlike PECAM1 its interaction with PIEZO1 is dynamic, increasing with shear stress. PIEZO1 does not interact with VGFR2. PIEZO1 is required in Ca2+-dependent formation of adherens junctions and associated cytoskeleton, consistent with it conferring force-dependent Ca2+ entry for junctional remodelling. The data suggest a pool of PIEZO1 at cell junctions, the coming together of PIEZO1 and PECAM1 mechanisms and intimate cooperation of PIEZO1 and adhesion molecules in tailoring junctional structure to mechanical requirement.
Long-term depression in neurons involves temporal and ultra-structural dynamics of phosphatidylinositol-4,5-bisphosphate relying on PIP5K, PTEN and PLC
Authors: Hofbrucker-MacKenzie Sarah A. , Seemann Eric , Westermann Martin , Qualmann Britta , Kessels Michael M. 
Published: April 2023

AbstractSynaptic plasticity involves proper establishment and rearrangement of structural and functional microdomains. Yet, visualization of the underlying lipid cues proved challenging. Applying a combination of rapid cryofixation, membrane freeze-fracturing, immunogold labeling and electron microscopy, we visualize and quantitatively determine the changes and the distribution of phosphatidylinositol-4,5-bisphosphate (PIP2) in the plasma membrane of dendritic spines and subareas thereof at ultra-high resolution. These efforts unravel distinct phases of PIP2 signals during induction of long-term depression (LTD). During the first minutes PIP2 rapidly increases in a PIP5K-dependent manner forming nanoclusters. PTEN contributes to a second phase of PIP2 accumulation. The transiently increased PIP2 signals are restricted to upper and middle spine heads. Finally, PLC-dependent PIP2 degradation provides timely termination of PIP2 cues during LTD induction. Together, this work unravels the spatial and temporal cues set by PIP2 during different phases after LTD induction and dissects the molecular mechanisms underlying the observed PIP2 dynamics.