bluuebunny/crossref_metadata_2025_split huggingface dataset:
The efficacy and safety of asleep and awake subthalamic deep brain stimulation for Parkinson’s disease patients: A 1-year follow-up
Authors: Chen Wanru , Zhang Changming , Jiang Nan , Jiang Lulu , Guo Qiyu , Gu Jing , Xian Wenbiao , Ling Yuting , Liu Yanmei , Zheng Yifan , Wu Lei , Yang Chao , Xu Shaohua , Hu Yu , Yang Yang , Chen Jinhua , Xuan Ruoheng , Liu Yi , Liu Jinlong , Chen Ling 
Published: April 2023

IntroductionTraditional DBS is usually conducted under local anesthesia (LA) which is intolerable to some patients, DBS under general anesthesia (GA) was opted to extended surgical indication. This study aimed to compare the efficacy and safety of bilateral subthalamic deep brain stimulation (STN-DBS) for Parkinson’s disease (PD) under asleep and awake anesthesia state in 1-year postoperative follow-up.MethodsTwenty-one PD patients were assigned to asleep group and 25 patients to awake group. Patients received bilateral STN-DBS under different anesthesia state. The PD participants were interviewed and assessed preoperatively and at 1-year postoperative follow-up.ResultsAt 1-year follow-up, compared surgical coordinate in two groups, the left-side Y of asleep group showed more posterior than awake group (Y was-2.39 ± 0.23 in asleep group, −1.46 ± 0.22 in awake group, p = 0.007). Compared with preoperative OFF MED state, MDS-UPDRS III scores in OFF MED/OFF STIM state remained unchanged, while in OFF MED/ON STIM state were significantly improved in awake and asleep groups, yet without significant difference. Compared with preoperative ON MED state, MDS-UPDRS III scores in ON MED/OFF STIM, and ON MED/ON STIM state remained unchanged in both groups. In non-motor outcomes, PSQI, HAMD, and HAMA score significantly improved in asleep group compared to awake group at 1-year follow-up (PSQI, HAMD, and HAMA score in 1-year follow-up were 9.81 ± 4.43; 10.00 ± 5.80; 5.71 ± 4.75 in awake group, 6.64 ± 4.14; 5.32 ± 3.78; 3.76 ± 3.87 in asleep group, p = 0.009; 0.008; 0.015, respectively), while there was no significant difference in PDQ-39, NMSS, ESS, PDSS score, and cognitive function. Anesthesia methods was significantly associated with improvement of HAMA and HAMD score (p = 0.029; 0.002, respectively). No difference in LEDD, stimulation parameters and adverse events was observed between two groups.DiscussionAsleep STN-DBS may be considered a good alternative method for PD patients. It is largely consistent with awake STN-DBS in motor symptoms and safety. Yet, it showed higher improvement in terms of mood and sleep compared to awake group at 1-year follow-up.
Brain Functional Alteration at Different Stages of Neuropathic Pain With Allodynia and Emotional Disorders
Authors: Zhang Ya-Nan , Xing Xiang-Xin , Chen Liu , Dong Xin , Pan Hao-Tian , Hua Xu-Yun , Wang Ke 
Published: April 2023

Graphical AbstractBrain functional alteration at different stages of neuropathic pain.
Optimizing Processing Technology of Cornus officinalis: Based on Anti-Fibrotic Activity
Authors: Han Xin , Ding Chuan , Ning Yan , Shan QiYuan , Niu Minjie , Cai Hao , Xu Peng , Cao Gang 
Published: April 2023

Graphical AbstractThe processing technology of C. officinalis was optimized in terms of its anti-fibrotic activity. Differences in its chemical composition after the preparation were detected by UHPLC-Q-TOF-MS/MS. These results provide material basis for further exploring the role of C. officinalis in liver and kidney protection.
Recent Advances in Dual PI3K/mTOR Inhibitors for Tumour Treatment
Authors: Wu Xianbo , Xu Yihua , Liang Qi , Yang Xinwei , Huang Jianli , Wang Jie , Zhang Hong , Shi Jianyou 
Published: April 2023

The PI3K-Akt-mTOR pathway is a viable target for cancer treatment and can be used to treat various malignant tumours, including follicular lymphoma and breast cancer. Both enzymes, PI3K and mTOR, are critical in this pathway. Hence, in recent years, an array of inhibitors targeting these two targets have been studied, showing dual PI3K/mTOR inhibition compared with single targeting small molecule inhibitors. Inhibitors not only inhibit cell proliferation but also promote cell apoptosis. These inhibitors show high potency and little drug resistance even at low doses, suggesting that PI3K/mTOR inhibitors are promising cancer drugs. Herein, we summarised the recent research of PI3K/mTOR dual inhibitors—for example, structure-activity relationship, pharmacokinetics, and clinical practice, and briefly commented on them.Clinical Trial Registration:https://clinicaltrials.gov.
Cerebroprotein hydrolysate attenuates neurodegenerative changes in Alzheimer’s mice model via ferroptosis pathway
Authors: Chen Moxi , Song Wei , Chen Zhengju , Shi Xiaodong , Wang Xue , Li Rongrong , Hao Honglin , Chen Wei 
Published: April 2023

Introduction:Cerebroprotein hydrolysate has been proven to improve cognitive function in patients with Alzheimer’s disease (AD). We explored the safety and effectiveness of the clinical administration of oral cerebroprotein hydrolysate in AD, and possible mechanisms related to the neuronal ferroptosis pathway.Methods:Three-month-old male APP/PS1 double-transgenic mice were randomly divided into AD model (n = 8) and intervention (n = 8) groups. Eight non-transgenic wild-type (WT) C57 mice were used as age-matched controls. The experiments were started at the age of 6 months. The intervention group was then administered cerebroprotein hydrolysate nutrient solution (11.9 mg/kg/day)viachronic gavage, the other groups received an identical volume of distilled water. Behavioural experiments were performed after 90 days of continuous administration. Serum and hippocampal tissues were then collected for histomorphological observation, tau and p-tau expression, and ferroptosis markers analysis.Results:Cerebroprotein hydrolysate simplified movement trajectories and shortened escape latencies of APP/PS1 mice in the Morris water maze test. Neuronal morphologies were restored in hippocampal tissues on haematoxylin-eosin staining. In the AD-model group, Aβ protein and p-tau/tau expression levels were elevated, plasma Fe2+and malondialdehyde levels were elevated, GXP4 protein expression and plasma glutathione levels declined than controls. All indices improved after cerebroprotein hydrolysate intervention.Conclusion:Cerebroprotein hydrolysate improves learning and memory function, alleviates neuronal damage, and reduces the deposition of pathological AD markers in AD mice, which may be related to the inhibition of neuronal ferroptosis.
High-resolution characterization of the coagulation and drying processes of whole blood based on optical coherence tomography
Authors: Zhuo Shufeng , Li Meifang , Lu Zenghai , Chen Kaihong 
Published: April 2023

Introduction: Escaping whole blood exhibits biochemical and physical coupled mechanisms such as coagulation and drying. However, there is no method for simultaneously monitoring the coagulation and drying procedure.Methods: In this study, a new method based on optical coherence tomography (OCT) combined with speckle variance and thickness is presented for simultaneously capturing spatially high-resolved characteristics of coagulation and drying of whole blood during the procedure. Deep learning based on a convolutional neural network (CNN) is employed for collecting OCT images with a resolution of micron order and quantitatively obtaining pixel-wise information of whole blood.Results and discussion: Then, the pixel-wise thickness map provides high-resolved temporal–spatial dynamics of whole blood during the drying procedure, and the corresponding speckle variance can uncover information of whole blood coagulation. The results demonstrate that coagulation and drying of whole blood have spatially inhomogeneous features. This method could provide the potential for revealing the coupling mechanism between coagulation and drying.
De novo transcriptome sequencing and gene co-expression reveal a genomic basis for drought sensitivity and evidence of a rapid local adaptation on Atlas cedar (Cedrus atlantica)
Authors: Cobo-Simón Irene , Gómez-Garrido Jèssica , Esteve-Codina Anna , Dabad Marc , Alioto Tyler , Maloof Julin N. , Méndez-Cea Belén , Seco José Ignacio , Linares Juan Carlos , Gallego Francisco Javier 
Published: April 2023

IntroductionUnderstanding the adaptive capacity to current climate change of drought-sensitive tree species is mandatory, given their limited prospect of migration and adaptation as long-lived, sessile organisms. Knowledge about the molecular and eco-physiological mechanisms that control drought resilience is thus key, since water shortage appears as one of the main abiotic factors threatening forests ecosystems. However, our current background is scarce, especially in conifers, due to their huge and complex genomes.MethodsHere we investigated the eco-physiological and transcriptomic basis of drought response of the climate change-threatened conifer Cedrus atlantica. We studied C. atlantica seedlings from two locations with contrasting drought conditions to investigate a local adaptation. Seedlings were subjected to experimental drought conditions, and were monitored at immediate (24 hours) and extended (20 days) times. In addition, post-drought recovery was investigated, depicting two contrasting responses in both locations (drought resilient and non-resilient). Single nucleotide polymorphisms (SNPs) were also studied to characterize the genomic basis of drought resilience and investigate a rapid local adaptation of C. atlantica.ResultsDe novo transcriptome assembly was performed for the first time in this species, providing differences in gene expression between the immediate and extended treatments, as well as among the post-drought recovery phenotypes. Weighted gene co-expression network analysis showed a regulation of stomatal closing and photosynthetic activity during the immediate drought, consistent with an isohydric dynamic. During the extended drought, growth and flavonoid biosynthesis inhibition mechanisms prevailed, probably to increase root-to-shoot ratio and to limit the energy-intensive biosynthesis of secondary metabolites. Drought sensitive individuals failed in metabolism and photosynthesis regulation under drought stress, and in limiting secondary metabolite production. Moreover, genomic differences (SNPs) were found between drought resilient and sensitive seedlings, and between the two studied locations, which were mostly related to transposable elements.DiscussionThis work provides novel insights into the transcriptomic basis of drought response of C. atlantica, a set of candidate genes mechanistically involved in its drought sensitivity and evidence of a rapid local adaptation. Our results may help guide conservation programs for this threatened conifer, contribute to advance drought-resilience research and shed light on trees’ adaptive potential to current climate change.
“PrEP protects us”: Behavioural, normative, and control beliefs influencing pre-exposure prophylaxis uptake among pregnant and breastfeeding women in Zambia
Authors: Hamoonga Twaambo Euphemia , Mutale Wilbroad , Hill Lauren M. , Igumbor Jude , Chi Benjamin H. 
Published: April 2023

BackgroundAlthough pre-exposure prophylaxis (PrEP) is recommended for pregnant and breastfeeding women at elevated HIV risk, uptake has been low in Zambia.MethodsIn in-depth interviews, we explored beliefs about PrEP among 24 HIV-negative pregnant and breastfeeding Zambian women. Thematic analysis was used to identify behavioural, normative and control beliefs likely to influence PrEP uptake.ResultsMost women viewed PrEP as a good method of protecting themselves and their babies from HIV infection. Partners were cited as key referents in decision making about PrEP use. Many women felt that PrEP use was not entirely in their control. Most reported that they would not use PrEP if their partners did not approve. Health care providers with negative attitudes, long distance to clinics, and extended waiting times were cited as barriers to PrEP uptake.ConclusionHIV-negative pregnant and breastfeeding women had a positive attitude towards PrEP but barriers to uptake are multifaceted.
Systematic hybrid laparoscopic and endovascular treatment of median arcuate ligament syndrome: A single-center experience
Authors: Schneider Michael , Longchamp Justine , Uldry Emilie , Corpataux Jean-Marc , Kefleyesus Amaniel , Halkic Nermin 
Published: April 2023

BackgroundMedian arcuate ligament syndrome (MALS) is caused by celiac trunk (CT) compression by the median arcuate ligament. Clinically, this pathology varies from postprandial pain (Dunbar syndrome) to a life-threatening hemorrhage because of a rupture of a gastroduodenal artery aneurysm (GAA). Due to the low prevalence of this disease, there is no standard management for MALS.Material and methodThis was a single-center, retrospective study of 13 patients. Two groups were identified on the basis of the initial clinical presentation: those operated for a GAA rupture (bleeding group—BG) and those operated electively for Dunbar syndrome (Dunbar syndrome group—DG). The primary endpoint was 30-day postoperative complications of a systematic laparoscopic release of the median arcuate ligament and stenting during the same procedure.ResultsSeven patients (54%) underwent elective surgery. Six patients (46%) underwent semiurgent repair under elective conditions post-embolization for GAA bleeding. The total operative time was longer in the BG (p = 0.06). Two patients in the BG suffered early major complications and needed reintervention, and those in the DG had a lower comprehensive complication index. No mortality was reported at 30 days. Overall median length of stay was 5 days (IQR: 3.5–15.3). Patients in the DG had a significantly shorter length of stay (p = 0.02). At 6 months, the primary and secondary CT stent patencies were 82% and 100%, respectively. There were no high-flow GAA recurrences.ConclusionsA combined approach of laparoscopic release of the median arcuate ligament and stenting during the same procedure is feasible and safe, and this approach must be systematically discussed in symptomatic patients.
Association of Circadian Clock Gene Expression with Pediatric/Adolescent Asthma and Its Comorbidities
Authors: Tran Nguyen Quoc Vuong , Le Minh-Khang , Nguyen Thuy-An , Kondo Tetsuo , Nakao Atsuhito 
Published: April 2023

The pathology of asthma is characterized by marked day–night variation, which is likely controlled by circadian clock activity. This study aimed to clarify the association of core circadian clock gene expression with clinical features of asthma. For this purpose, we accessed the National Center for Biotechnology Information database and analyzed transcriptomes of peripheral blood mononuclear cells and clinical characteristics of 134 pediatric/adolescent patients with asthma. Based on the expression patterns of seven core circadian clock genes (CLOCK, BMAL1, PER1-3, CRY1-2), we identified three circadian clusters (CCs) with distinct comorbidities and transcriptomic expressions. In the three CC subtypes, allergic rhinitis, and atopic dermatitis, both asthma comorbidities occurred in different proportions: CC1 had a high proportion of allergic rhinitis and atopic dermatitis; CC2 had a high proportion of atopic dermatitis but a low proportion of allergic rhinitis; and CC3 had a high proportion of allergic rhinitis but a low proportion of atopic dermatitis. This might be associated with the low activity of the FcεRI signaling pathway in CC2 and the cytokine–cytokine receptor interaction pathways in CC3. This is the first report to consider circadian clock gene expression in subcategories of patients with asthma and to explore their contribution to pathophysiology and comorbidity.
Dose–Response Efficacy and Mechanisms of Orally Administered Bifidobacterium breve CCFM683 on IMQ-Induced Psoriasis in Mice
Authors: Chen Xinqi , Chen Yang , Stanton Catherine , Ross Reynolds Paul , Zhao Jianxin , Chen Wei , Yang Bo 
Published: April 2023

This study aimed to investigate the dose–response effect of Bifidobacterium breve CCFM683 on relieving psoriasis and its underlying patterns. Specifically, the expression of keratin 16, keratin 17, and involucrin were substantially decreased by administration of 109 CFU and 1010 CFU per day. Moreover, interleukin (IL)-17 and TNF-α levels were substantially decreased by 109 and 1010 CFU/day. Furthermore, the gut microbiota in mice treated with 109 or 1010 CFU/day was rebalanced by improving the diversity, regulating microbe interactions, increasing Lachnoclostridium, and decreasing Oscillibacter. Moreover, the concentrations of colonic bile acids were positively correlated with the effectiveness of the strain in relieving psoriasis. The gavage dose should be more than 108.42 CFU/day to improve psoriasis according to the dose–effect curve. In conclusion, CCFM683 supplementation alleviated psoriasis in a dose-dependent manner by recovering microbiota, promoting bile acid production, regulating the FXR/NF-κB pathway, diminishing proinflammatory cytokines, regulating keratinocytes, and maintaining the epidermal barrier function. These results may help guide probiotic product development and clinical trials in psoriasis.
Identification and Evaluation of Novel Antigen Candidates against Salmonella Pullorum Infection Using Reverse Vaccinology
Authors: Jiang Zhijie , Kang Xiamei , Song Yan , Zhou Xiao , Yue Min 
Published: April 2023

Pullorum disease, caused by the Salmonella enterica serovar Gallinarum biovar Pullorum, is a highly contagious disease in the poultry industry, leading to significant economic losses in many developing countries. Due to the emergence of multidrug-resistant (MDR) strains, immediate attention is required to prevent their endemics and global spreading. To mitigate the prevalence of MDR S. Pullorum infections in poultry farms, it is urgent to develop effective vaccines. Reverse vaccinology (RV) is a promising approach using expressed genomic sequences to find new vaccine targets. The present study used the RV approach to identify new antigen candidates against Pullorum disease. Initial epidemiological investigation and virulent assays were conducted to select strain R51 for presentative and general importance. An additional complete genome sequence (4.7 Mb) for R51 was resolved using the Pacbio RS II platform. The proteome of S. Pullorum was analyzed to predict outer membrane and extracellular proteins, and was further selected for evaluating transmembrane domains, protein prevalence, antigenicity, and solubility. Twenty-two high-scored proteins were identified among 4713 proteins, with 18 recombinant proteins successfully expressed and purified. The chick embryo model was used to assess protection efficacy, in which vaccine candidates were injected into 18-day-old chick embryos for in vivo immunogenicity and protective effects. The results showed that the PstS, SinH, LpfB, and SthB vaccine candidates were able to elicit a significant immune response. Particularly, PstS confers a significant protective effect, with a 75% survival rate compared to 31.25% for the PBS control group, confirming that identified antigens can be promising targets against S. Pullorum infection. Thus, we offer RV to discover novel effective antigens in an important veterinary infectious agent with high priority.
Meniere’s Disease: The Current State of the Problem
Authors: Марьенко И.П. , Поддубный А.А. 
Published: April 2023

Болезнь Меньера (БМ) – многофакторное заболевание внутреннего уха, характеризующееся головокружением, шумом в ушах и нейросенсорной потерей слуха. Заболевание не имеет определенной этиологической причины. Развитие БМ зависит от целого ряда факторов, которые способны приводить к эндолимфатической водянке. Основным морфологическим признаком БМ является эндолимфатический гидропс – увеличение объема эндолимфы, заполняющей перепончатый лабиринт внутреннего уха, и растяжение Рейснеровой мембраны. Различают 3 основных варианта течения заболевания. Рост количества случаев БМ у лиц молодого возраста, а также отсутствие скрининга у лиц с повышенным риском (люди в возрастной категории от 20 до 50 лет со сниженным слухом на 1 или оба уха, с отягощенным семейным анамнезом по данному заболеванию, с травмами головы и/или уха, аутоиммунными заболеваниями) делает актуальной проблему разработки методов ранней диагностики и комплексного лечения БМ в Республике Беларусь.
Meniere’s disease (MD) is a multifactorial disease of the inner ear characterized by dizziness, tinnitus and sensorineural hearing loss. The disease has no definite etiological cause. The development of MD depends on a number of factors that can lead to the development of endolymphatic dropsy. The main morphological feature of MD is endolymphatic hydrops: an increase in the volume of the endolymph filling the membranous labyrinth of the inner ear, and stretching of the Reissner’s membrane. There are three main variants of the disease course. The increasing number of MD cases in young people, as well as the lack of screening in high-risk populations (individuals aged 20 to 50 years with impaired hearing in one or both ears, having family history aggravated with this disease, with head and/ or ear injuries, or with autoimmune diseases) make the issue of developing methods for early diagnosis and comprehensive treatment of MD in the Republic of Belarus relevant.
Secondary Musculoskeletal Disorders in Spastic Forms of Cerebral Palsy: A Literature Review
Authors: Венегас К.Ф. , Талабаев М.В. , Збанок И.Ю. , Забродец Г.В. , Соловьева А.Ю. 
Published: April 2023

В статье рассмотрены современные представления о фундаментальных предпосылках нарушения опорно-двигательного аппарата при детском церебральном параличе. Первичные двигательные нарушения возникают вследствие спастического синдрома, а затем в результате последующих патологических структурных изменений в мышцах, сухожилиях, капсульно-связочном аппарате суставов происходит формирование вторичных ортопедических осложнений со стороны нижних конечностей, позвоночника, нарушение позы и походки. Уровень спастичности напрямую определяет взаимосвязь между неврологическим и ортопедическим статусом детей с детским церебральном параличом. Все вышеперечисленные патофизиологические особенности, обусловленные спастичностью, приводят к прогрессирующему снижению физической активности пациентов, бытовой и социальной дезадаптации и ухудшению качества жизни не только пациентов, но и их семей в целом. Все это является предпосылкой для разработки и применения видов лечения, направленных на раннюю коррекцию спастического синдрома, с целью своевременного предупреждения развития вторичных ортопедических нарушений.
We discuss in this article modern ideas on the fundamental pathologies of the musculoskeletal system in cerebral palsy. Primary motor disorders occur as a result of spastic syndrome, followed by subsequent pathological structural changes in the muscles, tendons, capsular ligamentous apparatus of the joints resulting in secondary orthopedic complications in the lower extremities, spine, and posture and gait disorders. The level of spasticity directly determines the relationship between the neurological and orthopedic status of children with cerebral palsy. These pathophysiological features caused by spasticity lead to a progressive decrease in physical activity of patients, resulting in social maladaptation and deterioration of the quality of life for both patients and their families. All this is a prerequisite for developing and applying treatment methods aimed at early correction of spastic syndrome in order to timely prevent the development of secondary orthopedic disorders.