bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human airway epithelial cell pH regulatory proteins in cystic fibrosis: Differential expression in nasal and bronchial airway cells
Authors: Davis Michael D , Gaston Benjamin , Smith Laura , Rahman Rania Abdul , Khalid Faiza , Cotton Calvin , Chmiel James , Boyne Katie , Ramsey I. Scott , Marozkina Nadzeya 
Published: August 2023

Abstract
Background. Decreased apical bicarbonate transport into the airway surface liquid (ASL) has been associated with decreased ASL pH in some studies. Low ASL pH can have adverse respiratory effects. However, the human CF epithelium can also normalize ASL pH. We hypothesized that pH regulatory proteins other than the CF transmembrane regulator (CFTR) would be upregulated in the CF epithelium.
Methods. We grew primary human nasal and bronchial epithelial cells from healthy controls and CF subjects at air-liquid interface; each culture was grown until fully mature and ciliated (~ six weeks). We used immunoblotting to measure expression of proteins that can affect pH known in the airway, renal tubule and/or gut: carbonic anhydrases (CA) 1, 2 and 12; voltage-gated proton channel (Hv1); lactate dehydrogenases (LDH) A, B, and D; dual oxidases (DUOX) 1 and 2; Na+/H+ exchange regulatory factor; potassium-transporting ATPase alpha chain 2 (ATP12A), S-nitrosoglutathione reductase (GSNOR); glutaminase; and vacuolar-type ATPase (VTP-ase). Proteins that were differentially expressed were localized using cell fractionation and by immunofluorescence.
Results. There were no differences in expression of any of the proteins studied between healthy control and CF nasal epithelial cells. On the other hand, Hv1, CA1, CA2, CA12, LDHa, LDHd, ATP12a and GSNOR expression were each increased in the CF bronchial epithelium relative to the healthy control. Hv1 and CA2 were expressed in both cell membrane and cytoplasm, whereas CA1 and CA12 were cytoplasmic and nuclear. RNASeq data suggested that the differences in expression were post-transcriptional.
Conclusions. In the renal tubule and gut, mechanisms regulating luminal pH are well understood. In the airway, absence of CFTR can in some cases lead to ASL acidification; but other pH regulatory proteins are not well-studied. Here, we show increased expression of CA’s, LDH’s, ATP12a, GSNOR and Hv1 in bronchial epithelium from patients with CF. We speculate that these proteins could serve to normalize the CF epithelial pH. Nasal cells did not have these changes, arguing against the hypothesis that nasal epithelial gene expression results can uniformly be extrapolated to understand the biology of bronchial epithelium. These data also allow us to begin to create a map of pH regulatory systems in the human airway epithelium.
Dynamic and Static Soft Computation of Effluent Quality Parameters of Dissolved Air Flotation of an Industrial Wastewater Treatment Plant – A case study
Authors: Abouzari Milad , Pahlavani Parham , Ahmadi Hossein 
Published: August 2023

Abstract
In the present study, several static and dynamic intelligent computing methods, including Gene Expression Programming (GEP), newly approach of Robusted/ Least Squares Support Vector Machines (RO/LS-SVM) with Grid search and radial basic functions, robust LS-SVM with grid search, Focused Time Delay Neural Network (FTDNN) with Levenberg-Marquardt Learning Algorithm, FTDNN with Bayesian regularization Learning Algorithm, and FTDNN with Broyden-Fletcher-Goldfarb-Shanno (BFGS) Learning Algorithm have been used to estimate Acidity and Total dissolved solids (TDS) from the dissolved air flotation (DAF) unit. Furthermore, static and dynamic models perform differently under the conditions of shock loads accompanied by the temporal impact of past data on the system. Moreover, four related variables, such as COD, pH, Turbidity, and TDS from the effluent of the equalization tank (E. tank), were selected as inputs of the models, collected in an 8-hour monitoring during a year. The results demonstrated that GEP and FTDNN (with Bayesian regularization) with static and dynamic conditions modeled TDS and
pH parameters as the best results, respectively. The statistical quality of these models was found to be significant due to their high correlation between the experimental and observed chemical values, although some points in modeling, such as the implication of time lags, showed greater potential in model prediction along with simplicity and efficiency in a variety of complex physical, chemical, and biological processes.
Whole-family programmes for families living with parental mental illness: a systematic review and meta-analysis
Authors: Moltrecht Bettina , Lange Aurelie M.C. , Merrick Hannah , Radley Jessica 
Published: August 2023

Abstract
Several interventions have been developed to support families living with parental mental illness (PMI). Recent evidence suggests that programmes with whole-family components may have greater positive effects for families, thereby also reducing costs to health and social care systems. This review aimed to identify whole-family interventions, their common characteristics and investigate their effectiveness and acceptability. A systematic review was conducted according to PRISMA 2020 guidelines. A literature search was conducted in ASSIA, CINAHL, Embase, Medline and PsycINFO in January 2021 and updated in August 2022. We double screened 3914 abstracts and 212 papers according to pre-set inclusion and exclusion criteria. The Mixed Methods Appraisal Tool was used for quality assessment. Quantitative and qualitative data was extracted and synthesised. Randomised-control trial data on child and parent mental health outcomes was analysed separately in random-effects meta-analyses. The protocol, extracted data and meta-data are accessible via the Open Science Framework (https://osf.io/9uxgp/) Data from 67 articles, 42 trials and 31 interventions were included. Findings indicated small intervention effects for all outcomes including children’s and parents’ mental health (dc=-.017, -027; dp=-0.14, -0.16) and family outcomes. Qualitative evidence suggested that most families experienced whole-family interventions as positive, highlighting specific components as helpful, including whole-family components, speaking about mental illness, and the benefits of group settings. Our findings highlight the lack of high-quality studies. The present review fills an important gap in the literature by summarising the evidence for whole-family interventions. There is a lack of robust evidence coupled with a great need in families affected by PMI which could be addressed by whole-family interventions. We recommend the involvement of families in the further development of these interventions and in their evaluation.
Everything has changed: The impacts of the COVID-19 pandemic on the transit market in Montréal, Canada
Authors: Carvalho Thiago , Ahmed El-Geneidy 
Published: August 2023

Abstract

The COVID-19 pandemic has significantly impacted the transit market leading to ridership loss and service cuts. Most of the post-pandemic transit market literature has focused on how to attract those who stopped using transit services, however little attention has been given to how rider profiles have changed. To address this gap, we examine 2019 and 2022 data regarding transit commuters from Montréal, Canada. We apply factor and k-means cluster analyses to derive market segments at both points in time considering satisfaction levels, telecommuting rates, and frequency of transit use. We build upon these analyses to report on overall and mode group-level changes in the transit market. Our market segmentation reveals that captive, captive-by-choice, and choice riders still exist in the current public transit market. However, the share of these groups in the market has changed. The proportion of captive and choice riders has increased while captive-by-choice riders have shrunk in size. Moreover, the post-pandemic market has become mostly composed of infrequent riders and higher rates of telecommuting. We further explore these trends by commute mode (i.e., bus only, metro only, and bus and metro users). The findings from this research can be of interest to practitioners and policymakers as they shed light on the evolution of the perceptions and behaviours of segments of transit riders from before to after pandemic.
A Smartphone-based Crowd-sourced Real-time Surveillance Platform (Apple Snail Inspector) for the Invasive Snail Pomacea canaliculata: Design and Development Study
Authors: Dai Yang , Zhang Qiang , Ding Xin , Zhang Yingshu , Yang Yougui , Mao Fanzhen , Ni Bixian , Liu Yaobao , Culleton Richard , Cao Jun 
Published: August 2023

Abstract
Background
The large amphibious freshwater snail, Pomacea canaliculata (P. canaliculata) is an important invasive species in China, but there is currently no method available for its surveillance. The development and popularization of smartphones provides a new platform for research on surveillance technologies for the early detection and effective control of invasive species.
Objective
We describe the design and development of the “Apple Snail Inspector” (ASI), a mobile health (mHealth) surveillance system that features real-time reporting and mapping of P. canaliculata.
Methods
The ASI surveillance system was developed based on the infrastructure of the WeChat platform and Amap. The user can directly enter the game interface through the WeChat port on their mobile phone and the system automatically obtains their location. The user can then report the location of P. canaliculata. The administrator can audit the reported information, and all information can be exported to Microsoft Excel version 2016 for analysis. The map was generated by ArcGIS 10.2 and was used to characterize the spatial and temporal distribution of P. canaliculata in Jiangsu Province.
Results
The architecture of ASI consists of three parts: a mobile terminal, a server terminal, and a desktop terminal. We published more than 10 tweets on the official WeChat account of the system to announce it to the public and a total of 207 users in 2020 and 2021 correctly reported sightings of P. canaliculata. We identified 550 P. canaliculata breeding sites in 2020 and 2021, featuring ponds (81%), parks (17%), and farmland (2%). In addition, most of the locations contained snail eggs, and the reporting times mainly occurred between May and September.
Conclusions
The ASI is an effective surveillance system that can be used to identify the breeding locations of P. canaliculata, and provides the basis for its prevention and control. Its successful development and operation provides new potential avenues for surveillance of other public health issues.
Use of Graphene (GP)/Nylon 6 (PA6) nanocomposite for remediation of Cadmium (II) ions from aqueous solutions
Authors: Moja T. N. , Tsai T.-Y. , Mishra S. B , Hwang S. S. , Mishra AK 
Published: August 2023

Abstract

Graphene (GP) is reinforced with Nylon 6 (PA6) composites were synthesized by melt mix extrusion and moulded through Mucell® injection technique. PA6/GP nanocomposites were fabricated for adsorption application for removal of Cd(II) metal ion. The structural morphologies of all samples evaluated on XRD, SEM, and TGA illustrated the thermally stable, and crystallized materials with pore-like cells. SEM analysis at low magnifications showed neat PA6 experienced porous-like fracture and had rough surface after introduction of foaming agent, whilst nanocomposite (PA6/GP) samples had rough, grainy surface indicating extensive deformation within the matrix complex. The storage modulus can exhibit the stiffness of the material and tan δ indicates the glass transition temperature of the composites. The storage modulus is 2399, 2622, 2775, 3221 MPa at 60℃for the neat PA6, 0.5, 1, 3 wt% GP,
respectively. Furthermore, the removal of Cd(II) with PA6/GP 3.0 wt% composite was found to follow the Langmuir isotherm model with a 99.9 % removal efficiency. The results indicated that PA6 1.0 wt% composite can be efficiently used as a superabsorbent for the removal of Pb
(II) from aqueous solution.
Kidney Stone Disease and Osteoporosis: A Mendelian Randomization Study
Authors: He Qiushi , Huang Qingfeng , Hao Zongyao 
Published: August 2023

Abstract
Purpose
We analysed whether kidney stone disease is a risk factor for osteoporosis by conducting a two-sample Mendelian randomization study.
Methods
The SNPs associated with kidney stones were mainly derived from a large genome-wide association study that included 395,044 cases. We identified 46 SNPs that were considered to be strongly associated with the occurrence of kidney stones at a genome-wide significance level, independently inherited and without any linkage disequilibrium, and the above SNPs were selected as instrumental variables for this study. We selected several datasets on bone mineral density grouped by age and common measurement areas. A random-effects model using primarily IVW analysis was used to predict whether BMD levels were considered to change.
Results
The MR analysis shows that kidney stones were considered a possible cause of decreased total-body BMD (Beta=-3.5006, p-value = 0.0003) and in a subgroup analysis of the total-body BMD sample based on age grouping, the results showed that in the subgroup excluding the age grouping of 30 to 45 years, almost In all subgroups, kidney stones could be considered to be the cause of the decrease in total-body BMD. Among the specific sites of decreased BMD caused by kidney stones, the results showed that the occurrence of kidney stones caused a decrease in forearm BMD (p-value < 0.0001), heel BMD (p = 0.0088) and lumbar spine BMD (p = 0.0184).
Conclusion
This study supports renal stone disease as a risk factor for osteoporosis.
Application of PET imaging delta radiomics for predicting progression-free survival in rare high-grade glioma
Authors: Ahrari Shamimeh , Zaragori Timothée , Zinsz Adeline , Oster Julien , Imbert Laetitia , Verger Antoine 
Published: August 2023

Abstract

This study assesses the feasibility of using radiomics changes over time to predict progression-free survival in rare diseases. High-grade glioma patients (n = 53) underwent L-3,4-dihydroxy-6-[18F]-fluoro-phenylalanine (18F-FDOPA) positron emission tomography (PET) scans at the time of temozolomide chemotherapy discontinuation. Among these patients, 18 had previously undergone an18F-FDOPA PET during treatment. Radiomics features from static/dynamic parametric images, and conventional features, were extracted. After excluding highly correlated features, various feature selection methods and time-to-event survival algorithms were employed to generate 16 model combinations. Delta radiomics features, as relative and absolute changes, were either computed using informative features derived from the entire cohort or directly selected from the subset of 18 patients, and performances evaluated with the cross-validation technique. Delta-absolute radiomics achieved the highest performance when the pipeline was applied to the 18-patient subset (combination of support vector machine (SVM) and recursive feature elimination (RFE): C-index = 0.783 [0.744–0.818]). This result was comparable to using top-rank features from all 53 patients (SVM + RFE: C-index = 0.730 [0.700–0.760], p = 0.0034) and significantly outperformed delta-absolute conventional features (C-index = 0.584 [0.548–0.620], p < 0.001) and single-time-point radiomics features (C-index = 0.546 [0.512–0.580], p < 0.001). This highlights the considerable potential of delta radiomics to outperform delta conventional features and single-time-point radiomics features, in rare cancer cohorts.
Local-environment regulation engineering to boost acidic H2O2 electrosynthesis on cobalt porphyrin molecular electrocatalyst
Authors: Yang Hui , Chen Yihe , Zhen Cheng , Zhao Hao , Wang Yuda , Yue Zhouying , Wang Qiansen , Li Jun , Gu Meng , Cheng Qingqing 
Published: August 2023

Abstract
Electrosynthesis of hydrogen peroxide (H2O2) based on proton exchange membrane (PEM) reactor represents a promising route to industrial-level H2O2 production, while it is hampered by the lack of high-efficiency electrocatalysts in acidic medium. Herein, we present a local-environment regulation engineering to promote the H2O2 selectivity up to 92% on the structurally-defined cobalt porphyrin molecular catalyst through precisely-controlling specific oxygen functional group (OFG) on the reduced graphene oxide carrier. X-ray adsorption spectroscopy and Kelvin probe unravel that carboxyl and epoxy indirectly cause the charge-accumulation of Co center via delocalization of π-electrons on carbon plane, while hydroxyl directly induces the charge-depletion via axial coordination with Co center. Density functional theory illuminates the positive correlation between the electron density of Co center and H2O2 selectivity. At an industrial current density of 200 mA cm− 2, the optimized catalyst unprecedentedly achieves a steady H2O2 productivity of ~ 20 mol h− 1 gcat−1 in a flow cell and the continuous production of high-concentration (ca. 11 wt%) pure H2O2 in a PEM electrolyzer, suggesting a practical application potential in H2O2 industry.
Comparative Life Cycle Assessment for End-Of-Life Treatment of Expanded Polystyrene Produced in Street Markets of the Municipality of Thessaloniki, Greece
Authors: Christos Charalampidis , George Perkoulidis , Nicolas Moussiopoulos 
Published: August 2023

Abstract

Expanded polystyrene (EPS) is a useful material, often used as food packaging, mainly in the form of fish boxes. Though, after its useful life, EPS waste should be properly disposed of to avoid impacts on the environment or human health. In this study, three EPS end-of-life options are considered and compared for EPS waste produced in street markets of the Municipality of Thessaloniki, Greece: (a) collection and landfill disposal, (b) collection and recycling at a local facility and (c) collection, compacting and long-distance transport for recycling in Athens. Field research was carried out to obtain the total quantity of EPS waste produced per week. In general, recycling has favorable results, but certain trade-offs have been found. Transport has no significant effect for the scenarios in question. This is not the case with electricity consumption, which has a significant effect in shaping environmental impacts. Improvements regarding the country’s energy mix, which are already taking place, as well as minimization of water usage during or before recycling are important steps in achieving the maximum benefits of recycling.
The difference in clinical characteristics and outcomes between hospital acquired pleural infection and community acquired pleural infection
Authors: Zhu Qiang , Yu Mingzi , Zhang Xu , Du Mingmei , Cui Jiewei , Liang Zhixin 
Published: August 2023

Abstract
Background: This study intends to describe the clinical characteristics and microbiological features, drug resistance situations and outcomes associated with hospital acquired and community acquired pleural infection (PI) in a large Chinese tertiary-care hospital in Beijing, China.
Methods: The patients were divided into hospital acquired and community acquired pleural infection groups. The basic clinical data of the two groups were collected and
statistically analyzed, and the outcomes and prognostic factors of PI were summarized.
Results: There were 832 patients with PI over a history of 10 years in the hospital. Among them, 84.86% were hospital-acquired pleural infection (HAPI) and 15.14% were community-acquired pleural infection (CAPI). The most common cause of CAPI was pneumonia and tuberculous pleurisy, but malignant tumor, thoracic surgery and trauma were the most common cause of HAPI. The thoracic catheterization ≥10 days, abdominal catheterization, broad-spectrum antibiotics were used more than 2 days before PI, serum albumin and adenosine deaminase levels of HAPI and CAPI were statistically different. Gram-negative bacteria showed varying degrees of resistance to almost antibiotic, while there were hardly any Gram-positive bacteria resistant to linezolid and vancomycin except Enterococcus faecium, which has shown resistance to vancomycin(9.88%). The 30-day mortality after the onset of PI was 13.1%, and there was no significant statistical difference in prognosis between the patients with CAPI and HAPI. The multivariate logistics analysissuggested that ICU admission, solid tumor, chronic renal failure and decreasedserum albumin level were independent risk factors for PI.
Conclusion: There are differences in clinical characteristics between HAPI and CAPI, which should be paid more attention and got individualized treatment in clinical.
Genetic Preservation of SLC22A3 in Local South African Populations
Authors: Pearce Brendon , Jacobs Clifford , Benjeddou Mongi 
Published: August 2023

Abstract
Organic cation transporters of the amphiphilic solute facilitator family of membrane proteins are involved in the translocation of a diverse range of endogenous and exogenous organic cations. These organic cations include clinically important substrates such as the anti-diabetic drug metformin, the anti-neoplastic drug oxaliplatin, and the anti-retroviral drug lamivudine. Inter-individual variation in drug disposition and efficacy is a major clinical problem and the role of these transporters is increasingly being recognized as a possible mechanism explaining this variation. Moreover, it is estimated that between 15 and 30% of interindividual variation in drug disposition and response is a result of genetic factors The aim of this study was to determine the baseline minor allele frequency distribution of 18 known coding SNPs in the SLC22A3 gene of ~ 140 Cape Admixed and Xhosa individuals residing in Cape Town, South Africa. This study found no genetic polymorphisms in the coding region of the SLC22A3 gene of the Xhosa individuals investigated. This study has shown that SLC22A3 coding SNPs observed in other populations are absent in the sample of both Cape Admixed and Xhosa individuals studied. The lack of protein sequence variation was consistent with other studies and may reflect the significant physiological role of hOCT3 in maintaining cellular and organismal homeostasis.