bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough
Authors: Pavlíková Zuzana Čapková , Miletínová Petra , Roithová Adriana , Pospíšilová Klára , Záhonová Kristína , Kachale Ambar , Becker Thomas , Durante Ignacio M. , Lukeš Julius , Paris Zdeněk , Beznosková Petra , Valášek Leoš Shivaya 
Published: June 2023

SUMMARYtRNAs serve as a dictionary for the ribosome translating the genetic message from mRNA into a polypeptide chain. Besides this canonical role, tRNAs are involved in other processes like programmed stop codon readthrough (SC-RT). There, tRNAs with near-cognate anticodons to stop codons must outcompete release factors and incorporate into the ribosomal decoding center to prevent termination and allow translation to continue. However, not all near-cognate tRNAs promote efficient SC-RT. Here, we demonstrate that those that do, establish critical contacts between their anticodon stem (AS) and ribosomal proteins Rps30/eS30 and Rps25/eS25 forming the decoding site. Unexpectedly, the length and well-defined nature of the AS determines the strength of these contacts, which is reflected in organisms with reassigned stop codons. These findings open a new direction in tRNA biology that should facilitate the design of artificial tRNAs with specifically altered decoding abilities.
intI1 primer selection for class 1 integron integrase gene and transcript quantification – validation and application for monitoringintl1 gene abundance within septic tanks in Thailand
Authors: Okonkwo Valentine , Cholet Fabien , Ijaz Umer Z. , Koottatep Thammarat , Pussayanavin Tatchai , Polpraset Chongrak , Sloan William T. , Connelly Stephanie , Smith Cindy J. 
Published: June 2023

AbstractAntimicrobial resistance (AMR) poses serious global public health threat and wastewater treatment (WWT), including septic tanks, are a significant source of AMR genes to the environment. Environmental monitoring of broad-range AMR genes remains a challenge. The class-1 integron-integrase (intI1) gene has been proposed as a proxy for overall AMR abundance, but there is no consensus on the qPCR primer set to use. A systematic review of the literature found 65 primer sets. The coverage and specificity of each, including newly designed MGB-TaqMan primer-probe, was evaluated against a database of clinical and environmentalintl1, intl1-like and non-intl1 sequences. Three primer sets were selected, laboratory validated for DNA and mRNA quantification and used to quantityintl1 gene abundance from household and healthcare conventional septic tanks (CST) and novel household Solar Septic Tanks (SST) in Thailand. Specificity of theintl1septic tank amplicons showed that no primer set could distinguish betweenintl1andintl-1like sequences. Each primer set showed the same trends across septic tanks, with highest gene abundance in influent>sludge>effluent. There was no statistical difference between the same sample quantified by the three primer sets. However, when comparing gene abundances from the same primer set across septic tanks, statistical differences between different sample types were observed for one primer set but not the others. This may lead to different interpretation of risk associated with each reactor in spreadingintl1 to the environment. Comparing reactor types, intI1 abundance in the effluent was lowest in the SST-household<CST-household<CST-healthcare. Depending on primer set used, 31 to 42% ofintI1 was removed from effluent of the CST-household tank with accessible influent. Our study provided insight into the importance ofintl1primer choice. We propose the use of the validated set (F3-R3) for optimalintI1 quantification and towards the goal of achieving standardisation across environmental studies.
Novel carbon nanoparticles derived fromBougainvilleamodulate vegetative growth inArabidopsis
Authors: Barot Raviraj B , Gawande Nilesh D , Omprabha Satya , Kaushal Charli , Saha Jhuma , Bhatia Dhiraj , Sankaranarayanan Subramanian 
Published: June 2023

AbstractWe present a green synthesis method of producing blue fluorescence emitting carbon nanoparticles (CNPs) through a simple and cost-effective single-step hydrothermal reaction. The synthesis utilized bract extracts and pollen grains from threeBougainvilleaspecies:B. spectabilis, B. alba, andB. buttiana. The CNPs exhibited photoluminescence, with the highest emission observed in the ultraviolet region. Atomic force microscopy analysis revealed that the size of synthesized CNPs ranged from 23 nm to 83 nm. Fourier transform infrared analysis provided a comprehensive understanding of the CNP’s surface functional groups, with carbon being the predominant group. X-ray diffraction analysis confirmed the amorphous nature of the synthesized CNPs. Zeta potential measurements indicated that the particles carried a negative charge, suggesting their colloidal stability. In experiments conducted withArabidopsis thalianaseedlings, CNPs derived fromB. albapollen grains were found to promote leaf area expansion while simultaneously inhibiting primary root growth. Conversely, other CNPs demonstrated detrimental effects on vegetative growth. These findings underscore the potential application of these novel CNPs in agriculture.
Oligosaccharide production and signaling correlate with delayed flowering in anArabidopsisgenotype grown and selected in high [CO2]
Authors: Kinmonth-Schultz Hannah , Walker Stephen Michael , Bingol Kerem , Hoyt David W. , Kim Young-Mo , Markillie Lye Meng , Mitchell Hugh D. , Nicora Carrie D. , Taylor Ronald , Ward Joy K. 
Published: June 2023

AbstractSince industrialization began, atmospheric CO2([CO2]) has increased from 270 to 415 ppm and is projected to reach 800-1000 ppm this century. SomeArabidopsisecotypes delayed flowering in elevated [CO2] relative to current [CO2], while others showed no change or accelerations. To predict genotype-specific flowering behaviors, we must understand the mechanisms driving flowering response to rising [CO2]. [CO2] changes alter photosynthesis and carbohydrates in C3plants. Plants sense carbohydrate levels and exogenous carbohydrate application influences flowering time and flowering transcript levels. We asked how organismal changes in carbohydrates and transcription correlate with changes in flowering time under elevated [CO2]. We used a genotype (SG) ofArabidopsisthat was selected for high fitness at elevated [CO2] (700 ppm). SG delays flowering under elevated [CO2] (700 ppm) relative to current [CO2] (400 ppm). We compared SG to a closely related control genotype (CG) that shows no [CO2]- induced flowering change. We compared metabolomic and transcriptomic profiles in these genotypes at current and elevated [CO2] to assess correlations with flowering in these conditions. While both genotypes altered carbohydrates in response to elevated [CO2], SG had higher levels of sucrose than CG and showed a stronger increase in glucose and fructose in elevated [CO2]. Both genotypes demonstrated transcriptional changes, with CG increasing genes related to fructose 1,6-bisphosphate breakdown, amino acid synthesis, and secondary metabolites; and SG decreasing genes related to starch and sugar metabolism, but increasing genes involved in oligosaccharide production and sugar modifications. Genes associated with flowering regulation within the photoperiod, vernalization, and meristem identity pathways were altered in these genotypes. Elevated [CO2] may act through carbohydrate changes to influence transcription in both genotypes and delayed flowering in SG. Changes in the oligosaccharide pool may contribute to delayed flowering in SG. This work extends the literature exploring genotypic-specific flowering responses to elevated [CO2].
Research on the influence of online fitness user engagement on value co-creation behavior
Authors: Liu Gaofu , Yang Haonan , Nie Jing 
Published: June 2023

PurposeValue co-creation is a new initiative for enterprises to form a competitive advantage, and user engagement is the basis for achieving value co-creation; nevertheless, few studies have discussed the influence mechanisms of user engagement on value co-creation behavior. In this study, the authors aim to reveal the influence mechanisms of online fitness user engagement on value co-creation behavior by considering emotional resonance and immersive experience as mediating variables.Design/methodology/approachThe authors proposed and empirically tested a research model based on a survey involving 461 Chinese respondents through partial least squares structural equation modeling (PLS-SEM).FindingsThe results of this study confirm that consumer engagement, contributing engagement and social engagement are important drives of value co-creation behavior among online fitness users. Furthermore, emotional resonance and immersive experience have been revealed as important mediating mechanisms to explain why user engagement drives value co-creation behavior.Practical implicationsThe results of this study suggest that practitioners need to focus on the social engagement and consumer engagement of users in online fitness communities and to provide the appropriate environment and conditions for online fitness user to achieve mutual value co-creation.Originality/valueThis study makes two main contributions. It examines user engagement in an online fitness community context and helps to understand its applicability in other contexts. It explains the influence mechanisms of online fitness user engagement on value co-creation behavior and enriches the studies related to the drivers of value co-creation behavior.
Assessing smallholders’ heterogeneity towards sustainable agrifood value chain in developing countries
Authors: Hidayati Dwi Ratna , Garnevska Elena , Ramilan Thiagarajah 
Published: June 2023

PurposeAgrifood value chains in developing countries are transforming into higher value markets which require sustainable practices, with smallholders playing a critical role. However, smallholders are a heterogeneous group which may have discrepancies in outcomes to meet sustainability standards. This paper aims to empirically investigate smallholders' heterogeneity towards sustainable value chain practice in developing countries.Design/methodology/approachEight key enabling factors of sustainable value chain transformation were used to explore smallholders' typology, then profiled, based on their socio-economic status and current practices. A quantitative method was applied in Indonesia's cashew sector with 159 respondents from the primary producer area on Madura Island. A combination of descriptive analysis, cluster analysis, cross-tab analysis and one-way ANOVA analysis was used in this study.FindingsFour types of groups were identified, each with distinct characteristics and arranged in priority order as follows: accelerator, progressor, inattentive and conservative groups. Interventions can be implemented on per clusters basis or based on potential similarities among clusters, depending on priority. It is noted that the pursuit of sustainable value chain practices by smallholders is not necessarily associated with high socio-economic status, as those with low socio-economic status may have a stronger inclination towards them.Practical implicationsThe paper enhances awareness of practitioners and policymakers regarding smallholders' heterogeneity in sustainable value chain practice. It enables more effective and focused interventions to support smallholders who require assistance in sustainable production and value-adding activities. Different smallholders' characteristics call for different assistance/intervention. Practitioners can recognise smallholders' characteristics that are more compatible with higher value markets and sustainability requirements to better integrate their practices. Policymakers must carefully develop short-term and long-term interventions based on the activities prioritised by particular traits to “hit the right button” for smallholders' practice development.Originality/valueThis study investigates the typology of smallholders towards sustainable value chain practices by using eight enabling factors and profiling them based on their socio-economic condition and current practices. Additionally, this study shifts the focus of typology exploration away from the traditional lens of farm sustainability to a larger perspective which encompasses sustainable value chain activities.
Theory of green consumption behavior (TGCB): a case of agro-tourism for sustainable communities and cities of future Bangladesh
Authors: Hasan Abdulla Al-Towfiq 
Published: June 2023

PurposeThe purpose of this study is to explore the antecedents and their impacts on behaviors toward agro-tourism by proposing a theory of green consumption behavior.Design/methodology/approachBased on a review of the literatures and collection of 471 usable responses, the study is conducted through partial least squares structural equation modeling method using SmartPLS 3.3.3.FindingsFindings of the study have revealed that carbon mitigation attitude, energy saving norms, perceived hygiene value and agro-tourist spot visit intentions significantly influence agro-tourist spot visit behavior. Furthermore, the study has suggested that agro-tourist spot visit intentions partially mediate the strength of the relationship between carbon mitigation attitude, energy saving norms, perceived hygiene value and agro-tourist spot visit behavior.Practical implicationsThe study findings may be useful to encouraging agro-tourism managers of understanding antecedents of customer’s behavior and formulating business strategies influencing behaviors toward agro-tourism, coping with competitive business environment and environmental sustainability.Originality/valueThe study has presented a unique case discovering what antecedents are influencing customers’ green consumption behavior (agro-tourist spot visit behaviors) and how that behavior contributes to sustainable communities and cities development. Furthermore, the study has provided important insights for industry professionals by integrating carbon mitigation attitude, energy saving norms, perceived hygiene value and agro-tourist spot visit intentions in examining agro-tourist spot visit behavior.
Between accessibility and adaptability of digital platform: investigating learners' perspectives on digital learning infrastructure
Authors: Huda Miftachul 
Published: June 2023

PurposeThe massive expansion of digital platform has been responsible for the widespread progressive engagement created amongst learners and educators. The practice of requiring student feedback on online learning services ensures that teacher education continues to advance its strategic approach to online learning. This paper aims to examine the level of accessibility and adaptability of digital technology with particular focus on Malaysia, by elaborating the value of superior learning service and practical adaptability of online learning during the pandemic era.Design/methodology/approachThis study was conducted using qualitative approach of data collection, namely via structured interview. The listed respondents included 30 higher learners who participated in the study by providing feedback on the issues encountered during the research process.FindingsThe findings revealed that the strategic enhancement of digital accessibility continued with digital adaptability to sources of learning services would contribute to advancing achievement of digital learning pathway.Practical implicationsIncreasing accessibility to digital platforms in digital learning system can help to shape the digital environment. Digital expansion can create unlimited boundaries for online knowledge acquisition.Social implicationsThe social implication refers to acquiring the abilities developed through online engagement with peers by actualising and exploring information together with continuous inter-connectedness of sharing pathway in online platform. The instructor would need to give a proportional gateway to make learners experience the digital environment for future education.Originality/valueThis study aims to assess the value of developing accessibility of digital technology for students' online learning services during the pandemic and beyond. A well-structured plan would enable digital learning capabilities and mutual accessibility amongst learners. This can allow digital abilities to be transformed into collaborative teamwork amongst learners.
Geopolitical risk and corporate tax behavior: international evidence
Authors: Ramesh Vishnu K. , Athira A. 
Published: June 2023

PurposeThis study examines the association between geopolitical risk (GPR) and corporate tax, which is a major source of revenue for the government and a significant explicit cost for firms. The authors use a comprehensive measure of GPR to study its effects on corporate taxes by using an international sample.Design/methodology/approachThe authors adopt the geopolitical measure constructed by Caldara and Iacoviello (2022) as a proxy for GPR and cash-effective tax rate benchmarked with statutory tax rate to measure corporate tax avoidance. The authors employ panel regression with fixed effects (FEs) to investigate the impact of GPR on corporate tax avoidance. The authors also conduct a battery of robustness tests to ensure the strength of the study’s results.FindingsThis study’s empirical results indicate that sample firms increase their tax avoidance amid increasing GPR. Further analyses show that financial constraints incentivize firms to avoid taxes during rising geopolitical tensions. The authors also provide evidence on the role of firm-level and country-level governance in weakening the association between GPR and tax avoidance.Practical implicationsPolicymakers and governments may strengthen the enforcement rule to limit aggressive tax practices of corporates during GPR to balance fiscal deficit. In addition, this study sheds light on the debate among administrators and politicians over the efficacy of current tax laws and governance structures in the presence of heightened GPR.Originality/valueThe authors extend the literature on GPR by analyzing its effect on corporate tax avoidance. Unlike existing single-country studies, the authors use a cross-country setup to investigate the impact of GPR on tax avoidance, making this study’s results more generalizable as the authors control for a host of country, industry, and time factors. Apart from political uncertainty, terrorism, and climatic issues, the authors document GPR as a strong macroeconomic driver of corporate tax avoidance. The authors make a new contribution to the literature on the moderating role of governance and institutional factors on the association between tax avoidance and GPR in an international context. The authors also contribute to the literature on macroeconomic determinants of tax avoidance.
The mediating effect of knowledge management in the relationship between the HPO framework and performance
Authors: Bagorogoza Janet Kyogabiirwe , Herik Jaap van den , Waal Andrea de , Walle Bartel van de 
Published: June 2023

PurposeThe study examines the mediating effect of knowledge management (KM) in the relationship between the high-performance organisation (HPO) framework and high performance in financial institutions (FIs) in Uganda. The paper aims to develop a framework that promotes high performance in the FIs.Design/methodology/approachThe conceptual model was tested on a sample of 28 financial instituitions using structural equation model.FindingsThe findings revealed that the high-performance framework is significantly related to high performance and KM is related high performance. KM mediates the relationship between the high-performance framework and high performance.Research limitations/implicationsThe findings revealed that the high-performance framework is significantly related to high performance and KM is related high performance. KM mediates the relationship between the high-performance framework and high performance.Practical implicationsThe findings revealed that the high-performance framework is significantly related to high performance and KM is related high performance. KM mediates the relationship between the high-performance framework and high performance.Originality/valueThis study makes several empirical and theoretical contributions, addressing the gap in the literature about the role of the HPO framework in strategic management. This study tests the relationship between the HPO and the firm's performance by taking the mediating effects of KM. The designed model highlights a significant organisational performance approach that can influence the finance sector positively.
How should B2B firms create image content for high social media engagement? A multimodal analysis
Authors: Singh Shikha , Gandhi Mohina , Kar Arpan Kumar , Tikkiwal Vinay Anand 
Published: June 2023

PurposeThis study evaluates the effect of the media image content of business to business (B2B) organizations to accelerate social media engagement. It highlights the importance of strategically designing image content for business marketing strategies.Design/methodology/approachThis study designed a computation extensive research model based upon the stimulus-organism-response (SOR) theory using 39,139 Facebook posts of 125 organizations selected from Fortune 500 firms. Attributes from images and text were estimated using deep learning models. Subsequently, inferential analysis was established with ordinary least squares regression. Further machine learning algorithms, like support vector regression, k-nearest neighbour, decision tree and random forest, are used to analyze the significance and robustness of the proposed model for predicting engagement metrics.FindingsThe results indicate that the social media (SM) image content of B2B firms significantly impacts their social media engagement. The visual and linguistic attributes are extracted from the image using deep learning. The distinctive effect of each feature on social media engagement (SME) is empirically verified in this study.Originality/valueThis research presents practical insights formulated by embedding marketing, advertising, image processing and statistical knowledge of SM analytics. The findings of this study provide evidence for the stimulating effect of image content concerning SME. Based on the theoretical implications of this study, marketing and media content practitioners can enhance the efficacy of SM posts in engaging users.
The political budget cycles in emerging and developing countries
Authors: Nguyen Thanh Cong , Tran Thi Linh 
Published: June 2023

PurposeThis paper examines the political budget cycles in emerging and developing countries using a sample of 91 countries from 1992 to 2019.Design/methodology/approachThis paper employs a pooled ordinary least squares (OLS) model with clustered standard errors at the country level. To address endogeneity issues, the authors also employ a two-step system generalized methods of moments model.FindingsThe authors find clear evidence of political budget cycles in emerging and developing countries. The authors consistently find that incumbents increase total government spending, particularly in economic affairs, public services and social welfare, in the year before an election and the election year. In contrast, they contract spending in the year after an election.Research limitations/implicationsPolicymakers should be aware of the political budget cycles during election years. Promoting control of corruption and democracy helps to alleviate the effects of the political budget cycles in emerging and developing countries.Originality/valueThe authors are among the first to explore the political budget cycles in emerging and developing countries by focusing on the total government spending and its main compositions, including expenditures on economic affairs, public services and social welfare. Besides, the authors also explore the conditioning effects of control of corruption, political ideology and democracy.