bluuebunny/crossref_metadata_2025_split huggingface dataset:
CBD enhances the cognitive score of adolescent rats prenatally exposed to THC and fine-tunes relevant effectors of hippocampal plasticity
Authors: Castelli Valentina , Lavanco Gianluca , D’Amico Cesare , Feo Salvatore , Tringali Giuseppe , Kuchar Martin , Cannizzaro Carla , Brancato Anna 
Published: July 2023

Introduction: An altered neurodevelopmental trajectory associated with prenatal exposure to ∆-9-tetrahydrocannabinol (THC) leads to aberrant cognitive processing through a perturbation in the effectors of hippocampal plasticity in the juvenile offspring. As adolescence presents a unique window of opportunity for “brain reprogramming”, we aimed at assessing the role of the non-psychoactive phytocannabinoid cannabidiol (CBD) as a rescue strategy to temper prenatal THC-induced harm.Methods: To this aim, Wistar rats prenatally exposed to THC (2 mg/kg s.c.) or vehicle (gestational days 5–20) were tested for specific indexes of spatial and configural memory in the reinforcement-motivated Can test and in the aversion-driven Barnes maze test during adolescence. Markers of hippocampal excitatory plasticity and endocannabinoid signaling—NMDAR subunits NR1 and 2A-, mGluR5-, and their respective scaffold proteins PSD95- and Homer 1-; CB1R- and the neuromodulatory protein HINT1 mRNA levels were evaluated. CBD (40 mg/kg i.p.) was administered to the adolescent offspring before the cognitive tasks.Results: The present results show that prenatal THC impairs hippocampal memory functions and the underlying synaptic plasticity; CBD is able to mitigate cognitive impairment in both reinforcement- and aversion-related tasks and the neuroadaptation of hippocampal excitatory synapses and CB1R-related signaling.Discussion: While this research shows CBD potential in dampening prenatal THC-induced consequences, we point out the urgency to curb cannabis use during pregnancy in order to avoid detrimental bio-behavioral outcomes in the offspring.
A thematic analysis of bereaved adults' meaning-making experience of loss through playing video games
Authors: Eum Karam , Doh Young Yim 
Published: July 2023

IntroductionRecalling personal memories on the loss and deriving new meanings from them is deemed necessary for adapting to bereavement. Recent studies suggest that games can afford players meaningful experiences that can support players through stressful life events, but its potential on the meaning-making of loss has not been much explored. To address this gap, we investigated the bereaved players' experiences of playing commercial video games that elicited their personal memories of loss and what meanings they derived from those experiences.MethodTwelve adult players with bereavement experiences (six male, six female, age range: 20-31) played two video games (Bear's Restaurant and Spiritfarer). Their experiences during and after gameplay were tracked via play diaries and in-depth interviews. Data was analyzed using thematic analysis method.ResultsWe discovered seven themes on the meanings that players made from their gameplay experience: “Recalling memories”, “Avoiding engagement with the pain”, “Recognizing positive emotions”, “Acknowledging the deceased's perspective”, “Reviewing the meaning of loss”, “Planning a better future”, and “Fulfilling a wish”. Our findings indicate that bereaved players recalled and related their autobiographical memories to their in-game experiences. Furthermore, they derived new meanings on both the loss and their post-loss life after playing video games.DiscussionWe discuss how video games can provide a unique meaning-making experience to bereaved players by affording them an agency to actively reconstruct their narrative of loss and facilitating the sharing of grief.
Athletic performance, sports experience, and exercise addiction: an association study on ANKK1 gene polymorphism rs1800497
Authors: Bayraktar Isık , Cepicka Ladislav , Barasinska Magdalena , Kazan Hasan Huseyin , Zorba Erdal , Ergun Mehmet Ali , Eken Özgür , Ceylan Halil İbrahim , Bulgay Celal , Gabrys Tomasz 
Published: July 2023

IntroductionExercise addiction is a phenomenon being able to affecting the athletic performance. The gene, ANKK1 and the polymorphism NM_178510.2:c.2137G &gt; A (rs1800497) has been linked to the exercise addiction. However, further studies on diverse populations and sport branches are needed to totally explore the possible association of this polymorphism with the athletic performance. Thus, the present study aims to decipher any possible relations of the rs1800497 polymorphism with the athletic performance/personal best (PB) and sport experience of elite athletes.MethodsSixty volunteer elite athletes (31 sprint/power and 29 endurance) and 20 control/sedentary participated in the study. The polymorphism was genotyped using whole exome sequencing approach and PB were determined according to the International Association of Athletics Federations (IAAF) score.ResultsOur results underlined that there were not any significance differences for both allele and genotype frequencies between the groups in terms of athletic performance, although the frequency of allele G was higher (p &gt; 0.05). Nevertheless, sport experience significantly associated with the rs1800496 polymorphism (p &lt; 0.05).DiscussionIn conclusion, genotype G/G could be inferred to be linked to the higher sport experience and athletic performance. Still, further studies with higher number of participants are needed to conclude the association of this polymorphism with athletic parameters.
Physical activity and depression of Chinese college students: chain mediating role of rumination and anxiety
Authors: Liu Yanying , Feng Qingkun , Guo Kelei 
Published: July 2023

ObjectiveTo explore the relationship between physical activity and depression among college students, as well as the mediating role of rumination and anxiety.MethodsA total of 1,292 Chinese college students were investigated by physical activity questionnaire, rumination scale, self-rating anxiety scale (SAS), and depression scale.Results(1) There was a significant negative correlation between physical activity and depression (r = −0.399, p &lt; 0.01), and the direct path of physical activity on depression was significant (β = −0.399, t = −13.374, p &lt; 0.01). (2) Physical activity negatively predicted rumination (β = −0.322, t = −10.440, p &lt; 0.01) and anxiety (β = −0.222, t = −7.089, p &lt; 0.01). Rumination positively predicted anxiety (β = 0.283, t = 9.017, p &lt; 0.01) and depression (β = 0.267, t = 9.046, p &lt; 0.01). Anxiety positively predicted depression (β = 0.262, t = 8.902, p &lt; 0.01). (3) Rumination and anxiety play a significant mediating role between physical activity and depression. The mediating effect involves three paths: physical activity → rumination → depression (the mediating effect value: −0.076); physical activity → anxiety → depression (the mediating effect value: −0.052). Physical activity → rumination → anxiety → depression (the mediating effect value: −0.021).Conclusion(1) Physical activity can negatively predict the rumination, anxiety, and depression of college students, which means physical activity can reduce rumination, anxiety, and depression of college students. (2) Physical activity can not only directly affect the depression of college students, but also indirectly affect depression through the independent intermediary role of rumination and anxiety, and the chain mediation of rumination and anxiety.
Stigma and related factors among renal dialysis patients in China
Authors: Li Bing , Liu Di , Zhang Yue , Xue Pengshi 
Published: July 2023

BackgroundStigma is an important psychological concept that is being studied in many diseases. However, there have been few studies on stigma in renal dialysis patients in China. This study aimed to investigate the level of stigma and its potential influencing factors among Chinese renal dialysis patients.MethodsA cross-sectional study was conducted among renal dialysis patients in two Chinese dialysis centers between April 2022 and July 2022. Two hundred four renal kidney patients were interviewed with a questionnaire on demographic variables using the Social Impact Scale (SIS), Resilience Scale-14 (RS-14), Herth Hope Index(HHI), Multidimensional Scale of Perceived Social Support (MSPSS), Revised Life Orientation Test(LOT-R), Perceived Stress Scale (PSS-4) and Fear of Progression (FoP). T-test/univariate one-way ANOVA, Pearson’s R, and hierarchical linear regression analysis were used to investigate the factors that influence stigma.ResultsRenal dialysis patients in China experienced a moderate level of stigma (52.36 ± 8.16). Stigma was negatively correlated with resilience, hope, and perceived social support, whereas it was positively associated with perceived stress and fear of progression. However, it showed no significant relationship between optimism and stigma. Hierarchical linear regression analysis showed that hope (β = -0.318, P &lt; 0.001), social support (β = -0.193, P &lt; 0.01), perceived stress (β = 0.197, P &lt; 0.01), and fear of progression (β = 199, P &lt; 0.01) were found to be associated with stigma among the renal dialysis patients. All four variables in the model could explain 34.6% of the variance in stigma among renal dialysis patients in China.ConclusionAccording to this study, renal dialysis patients in China face a moderate level of stigma. Stigma was found to be negatively related to hope and social support but positively associated with perceived stress and fear of progression. Future research on the stigma of renal dialysis patients should include hope-based interventions, proper and specific social support strategies, stress management interventions, and more disease-related information.
Preoperative pelvic floor muscle exercise for continence after radical prostatectomy: a systematic review and meta-analysis
Authors: Zhou Luqiang , Chen Yu , Yuan Xiaojuan , Zeng Lijing , Zhu Jingzhen , Zheng Ji 
Published: July 2023

ObjectiveWe performed a systematic review and meta-analysis to evaluate the effect of preoperative pelvic floor muscle exercise on urinary incontinence after radical prostatectomy.MethodsWe searched the literature for randomized controlled trials evaluating the diagnostic analysis of preoperative pelvic floor muscle exercise (PFME) and postprostatectomy incontinence in the MEDLINE, EMBASE, PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), PsycINFO, China Biomedical Literature Database, China Journal Full-text Database, Wanfang Database and Weipu Database. The retrieval time limit is from the establishment of the database to January 2023. We used a risk ratio with accompanying 95% confidence interval (CI) to express estimates. Reviewer Manager (RevMan) 5.1.0 was used to complete all statistical analyses.ResultsTwelve studies were included based on the selection criteria. The total number of patients included in the final analysis was 1,365. At 1th month, there was no difference in continence rates between the groups [odds ratio (OR): 0.47; 95% confidence interval (CI), 0.22–1.02, p = 0.06]. At 3th month, there was statistically significant difference in PFME group before operation (OR: 0.61; 95% CI, 0.37–0.98, p = 0.04). At 6th and 12th months, there was no difference between groups (OR: 0.57; 95% CI, 0.28–1.17, p = 0.13), (OR: 0.56; 95% CI, 0.27–1.15, p = 0.12).ConclusionPreoperative pelvic floor muscle exercise can improve postoperative urinary incontinence at 3rd months after radical prostatectomy, but it cannot improve urinary incontinence at 6th months or longer after surgery, which indicates that preoperative PFME can improve early continence rate, but cannot improve long-term urinary incontinence continence rate.
Immunisation status of children receiving care and support in Wales: a national data linkage study
Authors: Bailey Grace A. , Lee Alexandra , Bedford Helen , Perry Malorie , Holland Sally , Walton Suzanne , Griffiths Lucy J. 
Published: July 2023

BackgroundIn the UK, a robust childhood immunisation programme ensures children are offered protection against serious infections; identifying inequalities in vaccination coverage is essential. This is one of the first data linkage studies to examine coverage of primary, as well as pre-school booster and second dose of MMR vaccines, in children receiving support from social care services across Wales.MethodsBy accessing records held within the Secure Anonymised Information Linkage (SAIL) Databank, vaccination status of children receiving social care and support between April 2016 and March 2021 (n = 24,540) was ascertained. This was achieved through linkage of the Children Receiving Care and Support (CRCS) Census and National Community Child Health Database which holds vaccination records for all children in Wales registered for NHS care. This sample was split into three groups – those children who had never been recorded on the Child Protection Register (CPR) or as ‘Looked After’ but in CRCS (n = 12,480), children ever on the CPR (n = 6,225) and those ever recorded as ‘Looked After’ but who were never on the CPR (n = 5,840). The comparison group of children and young people (CYP) never receiving welfare support consisted of 624,905 children.ResultsChildren receiving care or support were more likely to be up-to-date with all six vaccines (no recorded vaccines: 0.6–6.3%) compared to children in the comparison group (no recorded vaccines: 3–10.3%). However, of those who were vaccinated, they were less likely to be vaccinated in a timely manner; both early (5.2% vs. 22.2%; margin of error [ME] = 0.52, 95% CI [confidence interval] = −0.18 – −0.17, p &lt; 0.001) and delayed vaccinations were more common (62.7% vs. 71.3%; ME = 0.58, 95% CI = 0.08–0.09, p &lt; 0.001). Validation of the CRCS immunisation flag showed moderate levels of accuracy. Around 70% of immunisation flags were correct across all three groups.DiscussionFindings suggest a positive association between receiving services under a care and support plan and being up-to-date with immunisations; children receiving support under a care and support plan were more likely to have experienced early or late vaccinations, demonstrating that there is still more inter-disciplinary co-ordination and planning needed to improve these outcomes. Thus, identifying inequalities in vaccination coverage is essential to target interventions and to prioritise geographic areas for catch-up.
Simulation Study on Axial Location Identification of Damage in Layered Pipeline Structures Based on Damage Index
Authors: Li Ying , Qu Lingzhi , Qi Baoxin 
Published: July 2023

This study investigates the feasibility of identifying the axial position of circumferential defects in laminated pipeline structures based on damage indices. Wavelet packet decomposition is combined with damage indices, and the effects of dual defects with the same circumferential position but different axial positions, as well as dual defects with different circumferential and axial positions, on damage indices are separately studied. Our aim was to determine the potential to use damage indices to identify the axial position of circumferential defects in laminated pipeline structures. ABAQUS finite element analysis software was used to establish models of laminated pipeline structures with single defects and dual defects (with the same circumferential position but different axial positions, and with different circumferential and axial positions). The laminated pipeline structure was composed of a steel pipe (structural layer), a rigid polyurethane foam (insulation layer), and a high-density polyethylene (anticorrosion layer). The received sensing signals were averaged, and subjected to 5-level wavelet packet decomposition, to calculate the damage index values, which were then organized into a damage index matrix. Based on the trend of changes in the damage index matrix, the effects of variations in the number and circumferential position of the defects on the identification of the axial position of the damage were analyzed. The results indicate that the trend in damage index changes is influenced by the number of defects, and the increase in the circumferential distance between the second and the piezoelectric element sensor. This study found that when 1.7λ≤PD≤3.4λ, Idouble defect 90°<Isingle defect<Idouble defect 0°; when 3.7λ≤PD≤4λ, Idouble defect 90°<0.3<Idouble defect 0°<Isingle defect. This article demonstrates that the identification of the axial position of damage in laminated pipeline structures can be achieved using the damage index values in the damage index matrix. Additionally, this damage identification method overcomes the limitation of the wavelet packet’s inability to identify dual defects with relatively small relative axial distances. This provides new ideas and methods for finite element analysis in identifying the axial position of damage in laminated pipeline structures.
Mass Spectrometry Imaging of In Vitro Cryptosporidium parvum-Infected Cells and Host Tissue
Authors: Anschütz Nils H. , Gerbig Stefanie , Ghezellou Parviz , Silva Liliana M. R. , Vélez Juan Diego , Hermosilla Carlos R. , Taubert Anja , Spengler Bernhard 
Published: July 2023

Cryptosporidium parvum is a zoonotic-relevant parasite belonging to the phylum Alveolata (subphylum Apicomplexa). One of the most zoonotic-relevant etiologies of cryptosporidiosis is the species C. parvum, infecting humans, cattle and wildlife. C. parvum-infected intestinal mucosa as well as host cells infected in vitro have not yet been the subject of extensive biochemical investigation. Efficient treatment options or vaccines against cryptosporidiosis are currently not available. Human cryptosporidiosis is currently known as a neglected poverty-related disease (PRD), being potentially fatal in young children or immunocompromised patients. In this study, we used a combination of atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) mass spectrometry imaging (MSI) and liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to determine and locate molecular biomarkers in in vitro C. parvum-infected host cells as well as parasitized neonatal calf intestines. Sections of C. parvum-infected and non-infected host cell pellets and infected intestines were examined to determine potential biomarkers. Human ileocecal adenocarcinoma cells (HCT-8) were used as a suitable in vitro host cell system. More than a thousand different molecular signals were found in both positive- and negative-ion mode, which were significantly increased in C. parvum-infected material. A database search in combination with HPLC-MS/MS experiments was employed for the structural verification of markers. Our results demonstrate some overlap between the identified markers and data obtained from earlier studies on other apicomplexan parasites. Statistically relevant biomarkers were imaged in cell layers of C. parvum-infected and non-infected host cells with 5 µm pixel size and in bovine intestinal tissue with 10 µm pixel size. This allowed us to substantiate their relevance once again. Taken together, the present approach delivers novel metabolic insights on neglected cryptosporidiosis affecting mainly children in developing countries.
Coordination Polymers with a Pyrazine-2,5-diyldimethanol Linker: Supramolecular Networks through Hydrogen and Halogen Bonds
Authors: Armaghan Mahsa , Stürzer Tobias , Janiak Christoph 
Published: July 2023

In this paper, the synthesis and crystal structure of pyrazine-2,5-diyldimethanol (pyzdmH2, C6H8N2O2), a new symmetric water-soluble N,O-chelating tetra-dentate organic ligand, is reported and an environmentally friendly method is used to synthesize coordination compounds in water under ambient conditions, from the reaction of pyzdmH2 with the halide salts of Cu(II), Zn(II), Hg(II) and Cd(II): {[Cu(pyzdmH2)0.5(µ-Br)(Br)(H2O)]·H2O}n 1, {[Zn2(pyzdmH2)(µ-Cl)(Cl)3(H2O)]·H2O}n 2, [Hg2(pyzdmH2)0.5(µ-Cl)2(Cl)2]n 3, {[Cd2(pyzdmH2)(µ-Cl)4]·H2O}n 4, and {[Cd2(pyzdmH2)(µ-Br)4]·H2O}n 5. Single-crystal X-ray diffraction analysis reveals that 1–3 are 1D coordination polymers and 4 and 5 are 3D coordination networks, all constructed by bridging pyrazine-2,5-diyldimethanol and halogen ions. The hydroxyl groups in the organic linker extend the 1D chains to non-covalent 3D networks. In all non-covalent and covalent 3D networks, water molecules are trapped by strong hydrogen bond interactions. Supramolecular analysis reveals strong O-H···O, O-H···N, O-H···X, and weak C-H···O, C-H···X (X = Cl, Br) hydrogen bonds, as well as π-π(pyrazine ring), metal-halogen···π(pyrazine ring), and O-H···ring(5-membered chelate ring) interactions. In addition, X···O weak halogen bonds are present in 1–5 (X = Cl and Br).
Associations between β-Lactamase Types of Acinetobacter baumannii and Antimicrobial Resistance
Authors: Černiauskienė Kristina , Dambrauskienė Asta , Vitkauskienė Astra 
Published: July 2023

Background and objective: Acinetobacter baumannii (A. baumannii) is an important nosocomial pathogen that not only possesses intrinsic resistance to many classes of antibiotics, but is also capable of rapidly developing antimicrobial resistance during treatment. The aim of this study was to determine the characteristics of resistance of A. baumannii strains to β-lactams and other tested antibiotics, to evaluate the associations between the phenotypes of resistance to β-lactams and other tested antibiotics, and to evaluate the changes in antibiotic resistance of A. baumannii strains over 5 years by comparing the periods of 2016–2017 and 2020–2021. Materials and methods: A total of 233 A. baumannii strains were isolated from different clinical specimens of patients treated at the Hospital of Lithuanian University of Health Sciences in 2016–2017 (n = 130) and 2021–2022 (n = 103). All clinical cultures positive for A. baumannii were analyzed. The type of β-lactamase was detected by phenotypic methods using ESBL plus AmpC screen disk tests and the combination meropenem disk test. Results: In both periods, all A. baumannii strains were resistant to ciprofloxacin; resistance to carbapenems, piperacillin/tazobactam, gentamicin, and tobramycin was noted in more than 80% of strains. A comparison of two periods showed that the percentages of A. baumannii strains producing two or three types of β-lactamases were significantly greater in 2021–2022 than in 2016–2017 (94.2% and 5.8% vs. 17.7% and 2.3%, respectively, p < 0.001). Isolates producing two or three types of β-lactamases were more often resistant to tigecycline, tetracycline, and doxycycline than strains producing one type of β-lactamase (p < 0.001). Conclusions: The frequency of isolation of A. baumannii strains producing two different types of β-lactamases (AmpC plus KPC, AmpC plus ESBL, or ESBL plus KPC) or three types of β-lactamases (AmpC, KPC, and ESBL) and the resistance rates to ampicillin/sulbactam, tigecycline, tetracycline, and doxycycline were significantly greater in 2020–2021 as compared with 2016–2017. The production of two or three types of β-lactamases by A. baumannii strains was associated with higher resistance rates to tetracyclines.