bluuebunny/crossref_metadata_2025_split huggingface dataset:
MKRN1 promotes colorectal cancer metastasis by activating the TGF-β signalling pathway through SNIP1 protein degradation
Authors: Zhang Yi , Li Qin-shan , Liu Hong-lin , Tang Hong-ting , Yang Han-lin , Wu Dao-qiu , Huang Yu-ying , Li Li-cheng , Liu Li-hong , Li Meng-xing 
Published: August 2023

Abstract
Background
The Makorin ring finger protein 1 (MKRN1) gene, also called RNF61, is located on the long arm of chromosome 7 and is a member of the RING finger protein family. The E3 ubiquitin ligase MKRN1 is closely linked to tumour development, but the exact mechanism needs to be elucidated. In this study, we aimed to investigate the specific mechanism and role of MKRN1 in colorectal cancer (CRC) development.

Methods
MKRN1 expression in CRC was analysed using the Cancer Cell Line Encyclopaedia and the Cancer Genome Atlas (TCGA) databases. Rectal tumour tissues were frozen to explore the MKRN1 expression in CRC and its clinical significance. The impact of MKRN1 on CRC cell proliferation and migration was observed using CCK8, colony formation, wound healing, and transwell assays. A combination of MKRN1 quantitative proteomics, ubiquitination modification omics analysis, and a string of in vitro and in vivo experiments revealed the potential mechanisms by which MKRN1 regulates CRC metastasis.

Results
MKRN1 expression was significantly elevated in CRC tissues compared to paracancerous tissues and was positively linked with prognosis (P < 0.01). MKRN1 downregulation inhibits CRC cell proliferation, migration, and invasion. Conversely, MKRN1 overexpression promotes the proliferation, migration, and invasion of CRC cells. Mechanistically, MKRN1 induces epithelial-mesenchymal transition (EMT) in CRC cells via ubiquitination and degradation of Smad nuclear-interacting protein 1 (SNIP1). Furthermore, SNIP1 inhibits transforming growth factor-β (TGF-β) signalling, and MKRN1 promotes TGF-β signalling by degrading SNIP1 to induce EMT in CRC cells. Finally, using conditional knockout mice, intestinal lesions and metastatic liver microlesions were greatly reduced in the intestinal knockout MKRN1 group compared to that in the control group.

Conclusions
High MKRN1 levels promote TGF-β signalling through ubiquitination and degradation of SNIP1, thereby facilitating CRC metastasis, and supporting MKRN1 as a CRC pro-cancer factor. The MKRN1/SNIP1/TGF-β axis may be a potential therapeutic target in CRC.
Roles of follicle stimulating hormone and sphingosine 1-phosphate co-administered in the process in mouse ovarian vitrification and transplantation
Authors: Wang Fei , Tian Yuan , Huang Liwen , Qin Tian , Ma Wenye , Pei Chengbin , Xu Bo , Han Hang , Liu Xinrui , Pan Pengge , Yu Xiaoli , Chang Qin , Wang Yanrong , Zhang Shuya , Pei Xiuying 
Published: August 2023

AbstractSome major challenges of ovarian tissue vitrification and transplantation include follicle apoptosis induced by cryopreservation and ischemia-reperfusion injury, as well as ovarian follicle loss during post-transplantation. This research aimed to investigate the protective effects and underlying mechanisms of follicle-stimulating hormone (FSH) and Sphingosine-1-phosphate (S1P) on vitrified and post-transplantation ovaries. Ovaries from 21-day-old mice were cryopreservation by vitrification with 0.3 IU/mL FSH, 2 µM S1P, and 0.3 IU/mL FSH + 2 µM S1P, respectively, for follicle counting and detection of apoptosis-related indicators. The results demonstrated that FSH and S1P co-intervention during the vitrification process could preserve the primordial follicle pool and inhibit follicular atresia by suppressing cell apoptosis. The thawed ovaries were transplanted under the renal capsule of 6–8 week-old ovariectomized mice and removed 24 h or 7 days after transplantation. The results indicated that FSH and S1P co-intervention can inhibit apoptosis and autophagy in ovaries at 24 h after transplantation, and promote follicle survival by up-regulating Cx37 and Cx43 expression, enhanced angiogenesis in transplanted ovaries by promoting VEGF expression, as well as increased the E2 levels to restore ovarian endocrine function at 7 days after transplantation. The hypoxia and ischemia cell model was established by CoCl2 treatment for hypoxia in human granulosa-like tumor cell line (KGN), as well as serum-free culture system was used for ischemia. The results confirmed that ischemia-hypoxia-induced apoptosis in ovarian granulosa cells was reduced by FSH and S1P co-intervention, and granulosa cell autophagy was inhibited by up-regulating the AKT/mTOR signaling pathway. In summary, co-administration of FSH and S1P can maintain ovarian survival during ovarian vitrification and increase follicle survival and angiogenesis after transplantation.
Protocol of a multicenter, single-blind, randomized, parallel controlled trial evaluating the effect of microbiological rapid on-site evaluation (M-ROSE) guiding anti-infection treatment in patients with severe hospital-acquired pneumonia
Authors: Wang Xiuli , Wang Kaifei , Xie Fei , Han Zhihai , Liu Yuhong , Pan Lei , Zhu Guangfa , Cao Zhixin , Yan Peng , Xiao Li , Duan Zhimei , Hu Ye , Xiao Kun , Chen Xuxin , Fu Han , Shi Yinghan , Song Yuwei , Han Xiaobo , Xie Wuxiang , Xie Lixin 
Published: August 2023

Abstract
Introduction
The mortality rate of hospitalized patients with severe hospital-acquired pneumonia (SHAP) remains high. Empirical broad-spectrum antibiotic coverage and the misuse of high-grade antibiotics could lead to the emergence of multi-drug and even pandrug-resistant bacteria. In addition to metagenomic next-generation sequencing (mNGS), microbiological rapid on-site evaluation (M-ROSE) might be a useful technique to identify the pathogens in the early stage; however, the effect of M-ROSE guiding anti-infection treatment on prognostic outcomes of SHAP patients is still unclear.

Methods/design
This is a multicenter, single-blind, prospective, randomized controlled trial to evaluate the effect of M-ROSE guiding anti-infection treatment in SHAP patients, which will provide new strategies for the prevention and control of clinical multi-drug resistance bacteria. A total of 166 patients with SHAP, aged 18 years and over, will be recruited from seven centers in Beijing and randomly assigned to the intervention group (M-ROSE combined with mNGS) or the control group (mNGS only) in a 1:1 ratio using the central randomization system. Patients in the intervention group will accept M-ROSE and mNGS analysis, and the control group will accept mNGS analysis. Individualized anti-infective treatment and routine treatment will be selected according to the analysis results. The primary outcome is the ICU outcome (mortality). The safety of the intervention measures will be evaluated during the entire trial period. This trial will be the first randomized controlled trial to evaluate the effect of M-ROSE guiding treatment on mortality in patients with SHAP and may change the prevalence of multi-drug resistant bacteria.

Ethics and dissemination
This trial adheres to the Declaration of Helsinki and guidelines of Good Clinical Practice. Signed informed consent will be obtained from all participants. The trial has been approved by the Chinese PLA General Hospital (Approval Number: 20220322001).

Trial registration
ClinicalTrials.gov NCT05300776. Registered on 25 March 2022.
Study protocol: effects of treatment expectation toward repetitive transcranial magnetic stimulation (rTMS) in major depressive disorder—a randomized controlled clinical trial
Authors: Steiner Katharina M. , Timmann Dagmar , Bingel Ulrike , Kunkel Angelika , Spisak Tamas , Schedlowski Manfred , Benson Sven , Engler Harald , Scherbaum Norbert , Koelkebeck Katja 
Published: August 2023

Abstract
Background
Patients’ expectations toward any given treatment are highly important for the effectiveness of such treatment, as has been demonstrated for several disorders. In particular, in major depressive disorder (MDD), one of the most frequent and most serious mental disorders with severe consequences for the affected, the augmentation of available treatment options could mean a ground-breaking success. Repetitive transcranial magnetic stimulation (rTMS), a new, non-invasive, and well-tolerated intervention with proven effects in the treatment of MDD, appears particularly suitable in this context as it is assumed to exert its effect via structures implicated in networks relevant for both expectation and depression.

Methods
All patients will receive rTMS according to its approval. Half of the patients will be randomized to a psychological intervention, which is a comprehensive medical consultation aiming to improve positive treatment expectations; the control group will receive a conventional informed consent discussion (in the sense of a treatment-as-usual condition). As outcome parameters, instruments for both self-assessment and external assessment of depression symptoms will be applied. Furthermore, psycho-immunological parameters such as inflammation markers and the cortisol awakening response in saliva will be investigated. Resting-state functional magnetic resonance imaging (rs fMRI) will be performed to analyze functional connectivity, including the cerebellum, and to identify neuronal predictors of expectation effects. In addition, possible cerebellar involvement will be assessed based on a cerebellar-dependent motor learning paradigm (i.e., eyeblink conditioning).

Discussion
In this study, the effects of treatment expectations towards rTMS are investigated in patients with MDD. The aim of this study is to identify the mechanisms underlying the expectation effects and, beyond that, to expand the potential of non-invasive and well-tolerated treatments of MDD.

Trial registration
German Registry of Clinical Studies (DRKS DRKS00028017. Registered on 2022/03/07. URL: https://www.drks.de/drks_web/.
Intravesical urachal cyst masquerading as a bladder malignancy: a case report
Authors: Alyusuf Zahra , Hassan Ali , Maki Reem , Hasan Wafa , Alhamar Roaa 
Published: August 2023

Abstract
Background
Urinary bladder masses in children are extremely rare. Certain benign conditions (e.g., ureterocele) can mimic malignant bladder masses. In this report, we present a unique case of a urachal cyst masquerading as a bladder malignancy. Unlike the typical location of urachal cysts along the course of the urachal tract, the cyst in this case was unexpectedly situated within the urinary bladder, leading to diagnostic difficulties.

Case presentation
A 2-year-old Bahraini boy presented with hematuria and dysuria for 2 weeks. There was no history of fever, abdominal pain, or vomiting. Physical examination yielded normal findings. Urinalysis showed numerous red blood cells and revealed positive results for nitrites and leukocyte esterase. Abdominal ultrasound showed a well-defined soft tissue lesion with internal vascularity located at the apex of the urinary bladder. Subsequently, magnetic resonance imaging demonstrated a thick-walled cystic structure arising from the anterosuperior wall of the bladder and protruding into its lumen. The patient underwent complete excision of the bladder lesion for the presumed diagnosis of rhabdomyosarcoma. Histopathological examination showed a fluid-filled space lined by stratified squamous epithelium with areas of intestinal metaplasia, revealing an unexpected diagnosis of a urachal cyst. The patient was discharged with complete resolution of symptoms.

Conclusions
Intravesical urachal cysts are a rare type of congenital urachal anomaly that may simulate a bladder malignancy, particularly if associated with infection. This case emphasizes the importance of considering urachal cysts in the differential diagnosis of bladder masses, especially in children, and specifically when the lesion is midline in the anterosuperior wall of the bladder.
Cell-free fat extract restores hair loss: a novel therapeutic strategy for androgenetic alopecia
Authors: Cai Yizuo , Jia Zhuoxuan , Zhang Yichen , Kang Bijun , Chen Chingyu , Liu Wei , Li Wei , Zhang Wenjie 
Published: August 2023

AbstractBackgroundAndrogenetic alopecia (AGA) is one of the most common hair loss diseases worldwide. However, current treatments including medicine, surgery, and stem cells are limited for various reasons. Cell-free fat extract (CEFFE), contains various cell factors, may have potential abilities in treating AGA. This study aims to evaluate the safety, effectiveness and the underlying mechanism of CEFFE in treating AGA.MethodsSex hormone evaluation, immunogenicity assay and genotoxicity assay were conducted for CEFFE. In vivo study, male C57BL/6 mice were injected subcutaneously with dihydrotestosterone (DHT) and were treated with different concentration of CEFFE for 18 days (five groups andn = 12 in each group: Control, Model, CEFFELow, CEFFEMiddle, CEFFEHigh). Anagen entry rate and hair coverage percentage were analyzed through continuously taken gross photographs. The angiogenesis and proliferation of hair follicle cells were evaluated by hematoxylin–eosin, anti-CD31, and anti-Ki67 staining. In vitro study, dermal papilla cells (DPCs) were incubated with different concentrations of CEFFE, DHT, or CEFFE + DHT, followed by CCK-8 assay and flow cytometry to evaluate cell proliferation cycle and apoptosis. The intracellular DHT level were assessed by enzyme-linked immunosorbent assay. The expression of 5α-reductase type II, 3α-hydroxysteroid dehydrogenase and androgen receptor were assessed through reverse transcription-quantitative polymerase chain reaction (RT-qPCR) or/and western blot.ResultsIn CEFFE-treated mice, an increase in the anagen entry rate and hair coverage percentage was observed. The number of CD31-positive capillaries and Ki67-positive cells were increased, suggesting that CEFFE promoted the proliferation of DPCs, modulated the cell cycle arrest, inhibited apoptosis caused by DHT, reduced the intracellular concentration of DHT in DPCs, and downregulated the expression of AR.ConclusionsCEFFE is a novel and effective treatment option for AGA through producing an increased hair follicle density and hair growth rate. The proposed mechanisms are through the DHT/AR pathway regulation and regional angiogenesis ability.
Is it possible to control bovine tuberculosis without compensation? Reviewing ten years of the Chilean program and its progress
Authors: Valdivieso Nicolás , Retamal Patricio 
Published: August 2023

AbstractIn 2011, the Chilean bovine tuberculosis (bTB) program was launched by the Livestock and Agriculture Service (SAG) as a compulsory countrywide program based on testing and culling of bTB reactors at herd-owners expense. This review outlines the rationale and key components of the bTB program, and the dynamic changes that have occurred since 2011. The paper also examines the problems identified by stakeholders and the initiatives put in place to address the constraints to achieving progress.To date, the program has shown progress in controlling bTB. However, in order to achieve bTB eradication it will be essential to improve the commitment of stakeholders, and to develop a framework of strong and workable regulations that will help to manage bTB outbreaks, particularly where clusters of bTB infection are recorded.
Meta-analysis of food supply chain: pre, during and post COVID-19 pandemic
Authors: Kafi Abdul , Zainuddin Nizamuddin , Saifudin Adam Mohd , Shahron Syairah Aimi , Razalli Mohd Rizal , Musa Suria , Ahmi Aidi 
Published: August 2023

Abstract
Background
Despite the unprecedented impact of COVID-19 on the food supply chain since 2020. Understanding the current trends of research and scenarios in the food supply chain is critical for developing effective strategies to address the present issue. This study aims to provide comprehensive insights into the pre, during, and post COVID-19 pandemic in the food supply chain.

Methodology
This study used the Scopus database from 1995 to November 6, 2022, to analyse the food supply chain. Bibliometric analysis was conducted using VOSviewer software to create knowledge maps and visualizations for co-occurrence, co-authorship, and country collaboration. Biblioshiny, a shiny app for the Bibliometrix R package, was then used to explore theme evaluation path maps in the research domain.

Results
The bibliometric analysis of 2523 documents provides important insights into present and future publication trends. Top author keywords included blockchain, traceability, food safety, sustainability, and supply chain management. The Sustainability (Switzerland) journal ranked first in productivity, and the International Journal of Production Economics received the highest citations. The United Kingdom was the most productive country, collaborating with partners in Europe, Asia, and North America. The Netherlands had the highest percentage of documents with international authors, while India and China had the lowest. The thematic evaluation maps revealed that articles focused on important research topics including food processing industry, information sharing, risk assessment, decision-making, biodiversity, food safety, and food waste.

Conclusion
This study contribute to the growing body of literature on the food supply chain by providing a comprehensive analysis of research trends during different phases of the pandemic. The findings can be used to inform policymakers and industry leaders about the measures required to build a more resilient and sustainable food supply chain infrastructure for the future. This study considered only Scopus online database for bibliometric analysis, which may have limited the search strategy. Future studies are encouraged to consider related published articles by linking multiple databases.
Effect of Contents, Tensile Strengths and Aspect Ratios of Hooked-End Steel Fibers (SFs) on Compressive and Flexural Performance of Normal Strength Concrete
Authors: Jang Seok-Joon , Park Wan-Shin , Kim Sun-Woo , Kim Dong-Hui , Wang Qi , Jeong Woo-Jin , Jin Ai-Hua , Yun Hyun-Do 
Published: August 2023

AbstractThis study is a part of the study to simplify the reinforcing details of reinforced concrete (RC) structural members by substituting the conventional reinforcement with hooked-end steel fibers (SFs). This paper investigates the effects of SF strength, dosage and aspect (l/d) ratio on the compressive and flexural behaviors of normal strength concrete with specified compressive strength of 30 MPa. In this study, hooked-end SFs of high strength (2000–2400 MPa) and normal strength (1100–1200 MPa) were used with three l/d ratios of 64, 67 and 80. Hooked-end SFs were incorporated with three dosages of 20 kg/m3 (0.25 vol.%), 40 kg/m3 (0.50 vol.%) and 60 kg/m3 (0.75 vol.%). Eighteen steel fiber reinforced concrete (SFRC) mixes were mixed. To evaluate the compressive and flexural performance of each SFRC mixture, three SFRC cylindrical and prismatic specimens for each mixture were manufactured and tested, respectively. The test results that the inclusion of hooked-end SFs had little effect on the compressive strength, while it improved the toughness of concrete. Hooked-end SFs were also found to be effective in enhancing the flexural performance of concrete. The dosage and properties (strength and l/d ratio) of SFs significantly affect the residual flexural tensile strength (fR1 and fR3) at serviceability (SLS) and ultimate limit state (ULS) defined in fib Model Code 2010 (MC2010).
Gill-associated bacteria are homogeneously selected in amphibious mangrove crabs to sustain host intertidal adaptation
Authors: Fusi Marco , Ngugi David K. , Marasco Ramona , Booth Jenny Marie , Cardinale Massimiliano , Sacchi Luciano , Clementi Emanuela , Yang Xinyuan , Garuglieri Elisa , Fodelianakis Stilianos , Michoud Grégoire , Daffonchio Daniele 
Published: August 2023

Abstract
Background
The transition from water to air is a key event in the evolution of many marine organisms to access new food sources, escape water hypoxia, and exploit the higher and temperature-independent oxygen concentration of air. Despite the importance of microorganisms in host adaptation, their contribution to overcoming the challenges posed by the lifestyle changes from water to land is not well understood. To address this, we examined how microbial association with a key multifunctional organ, the gill, is involved in the intertidal adaptation of fiddler crabs, a dual-breathing organism.

Results
Electron microscopy revealed a rod-shaped bacterial layer tightly connected to the gill lamellae of the five crab species sampled across a latitudinal gradient from the central Red Sea to the southern Indian Ocean. The gill bacterial community diversity assessed with 16S rRNA gene amplicon sequencing was consistently low across crab species, and the same actinobacterial group, namely Ilumatobacter, was dominant regardless of the geographic location of the host. Using metagenomics and metatranscriptomics, we detected that these members of actinobacteria are potentially able to convert ammonia to amino acids and may help eliminate toxic sulphur compounds and carbon monoxide to which crabs are constantly exposed.

Conclusions
These results indicate that bacteria selected on gills can play a role in the adaptation of animals in dynamic intertidal ecosystems. Hence, this relationship is likely to be important in the ecological and evolutionary processes of the transition from water to air and deserves further attention, including the ontogenetic onset of this association.
Cross-cultural adaptation and psychometric properties of the Italian version of the Orthorexia Nervosa Inventory (ONI)
Authors: Zagaria Andrea , Barbaranelli Claudio , Mocini Edoardo , Lombardo Caterina 
Published: August 2023

Abstract
Background
Orthorexia Nervosa (ON) is an emerging clinical condition characterized by a pathological fixation with healthy eating. Recently, the Orthorexia Nervosa Inventory (ONI) has emerged as a promising tool for assessing orthorexic tendencies and behaviours, aiming to overcome the well-established limitations of existing measures for ON. The present study aimed to examine the psychometric properties of the Italian version of the ONI.

Methods
A total of 879 participants (Mage = 33.22 years, SD = 9.19; 56.9% females) completed the ONI along with the Düsseldorf Orthorexia Scale (DOS), measures of disordered eating, obsessive-compulsive symptoms (OCD), and psychosocial impairment. To establish the factorial validity of the ONI, a competing measurement modeling approach was employed by comparing standard confirmatory factor analytic (CFA) with exploratory structural equation modeling (ESEM) solutions. Model-based omega coefficients were computed to examine the internal consistency of the scale. Factorial invariance tests across gender were conducted within a multi-group framework.

Results
A three-factor first-order ESEM solution provided the best and most parsimonious representation of the data: χ2(207) = 558.641, p < .001, RMSEA = 0.044 (90% CI 0.040–0.048), CFI = 0.976, TLI = 0.968, SRMR = 0.036. The three latent dimensions, labelled behaviors, emotions, and impairments, showed excellent internal consistency (ω > 0.88). Furthermore, ONI scores were found to be positively correlated with DOS scores, disordered eating, OCD symptoms, and psychosocial impairment, supporting its convergent and criterion validity. Eventually, the ONI was factorially invariant across gender.

Conclusions
Overall, the present study provides evidence for the satisfactory psychometric properties of the ONI in the Italian context, endorsing its use in both clinical and research settings.