bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interseeding cover crop into an irrigated sandy loam for 6 years: Soil, crop, and economic response
Authors: Blanco‐Canqui Humberto , Ruis Sabrina J. , Mamo Mitiku , Shapiro Charles A. , Proctor Christopher , Parsons Jay , Thompson Laura 
Published: January 2025

AbstractInterseeding cover crops (CCs) may be a potential strategy to manage sandy soils, which are highly prone to degradation. However, how this practice affects CC biomass production and other ecosystem services in sandy soils over the traditional CC planting system (post‐harvest drilling) is still unclear. We studied how broadcast interseeded (32–67 days before crop harvest) winter rye (Secale cereale L.) CC affected CC biomass production, nitrate leaching potential, soil properties, crop yields, and farm income compared with post‐harvest drilled CC in an on‐farm irrigated no‐till corn (Zea mays L.)–soybean (Glycine max L.) experiment in a sandy loam in the western US Corn Belt for 6 years. Across the 6 years, interseeded CC produced 0.57 Mg ha−1 of biomass, while post‐harvest drilled CC produced 0.37 Mg ha−1. Nitrate leaching is a concern in sandy soils, but interseeded CC had mixed effects on soil nitrate concentration. Interseeded CC did not affect soil properties (particulate organic matter and organic C concentrations, and wet aggregate stability) and crop yields. Further, interseeded CC did not reduce farm income more than post‐harvest drilled CC. The limited effect of interseeded CCs is likely due to the relatively small increase in CC biomass production over the traditional CC planting system. Additional strategies including irrigation, drill interseeding, and planting green may boost interseeded CC biomass production and thus soil services in sandy soils. After 6 years, interseeded CC slightly boosted CC biomass production but minimally affected soils and crops, and both interseeded and post‐harvest‐drilled CCs reduced net income.
Unveiling the role of mixing in [Fe(CN)6]4− defects and Na content for preparing NaxMn[Fe(CN)6]y · nH2O via microreactor
Authors: Tong Hao‐Tian , Wei Shi‐Xiao , Liu Yan‐Jiang , Xie Ting‐Liang , Yin Shuang‐Feng 
Published: January 2025

AbstractTaking advantage of strong mixing performance in oscillating feedback microreactor (OFM), the manganese hexacyanoferrates (NaxMn[Fe(CN)6]y · nH2O, MnHCFs) with low defects and high sodium content were controllably prepared. First, fluid mixing performance in OFM was investigated via dye‐tracer and Villermaux‐Dushman experiments, and the fluid mixing mechanism in OFM was investigated through CFD simulations. Then, MnHCFs were prepared using OFM at different flow rates, and the role of mixing in [Fe(CN)6]4− vacancies and sodium content during co‐precipitation synthesis of MnHCFs was discussed. The results indicate that an increase in flow rates can increase fluid chaos strength, enhancing the mass transfer process to match the extremely fast reaction rate of MnHCFs, thus preparing MnHCFs with high sodium content and low defects. Relevant electrochemical tests indicate that the MnHCF prepared at the largest throughput (180 mL/min) has the highest initial specific capacity (132 mAh/g at 15 mA/g), minimum electrochemical impedance, and fastest sodium ion transport rate.
Genetic Correlations Among Dental, Mandibular, and Postcranial Dimensions in Rhesus Macaques (Macaca mulatta)
Authors: Hardin Anna M. 
Published: January 2025

ABSTRACTObjectivesTooth dimensions typically scale with mandibular and postcranial size in primates, although the exact pattern of scaling varies. This study assesses whether correlations by tissue type, anatomical region, or function (mastication or intrasexual competition) are present and could therefore act as evolutionary constraints on tooth‐jaw‐body size relationships by estimating genetic and phenotypic correlations between dental, mandibular, and postcranial dimensions in rhesus macaques (Macaca mulatta).Materials and MethodsThe teeth, mandibles, and postcrania of 362 adults from the Cayo Santiago skeletal collection were measured. Humeral and femoral articular surfaces were selected to represent skeletal elements frequently used to reconstruct primate body size. Genetic correlations were estimated in SOLAR. Random skewers analyses were used to compare genetic and phenotypic correlation matrices to each other and to test matrices.ResultsThe genetic correlation matrix was most similar to the test matrix of integration by tissue type (tooth and bone) (r = 0.765, p < 0.001), and nearly as similar to the anatomical region test matrix (r = 0.714, p < 0.001). Hierarchical clustering of the genetic correlation matrix showed similar separation by tissue type. Phenotypic correlation matrices were significantly similar to the genetic correlation matrix (r = 0.813–0.846, p < 0.001) and to most of the test matrices.ConclusionsPostcanine dental traits were highly heritable but were not closely genetically correlated with mandibular or postcranial dimensions. These findings indicate that scaling relationships between tooth and bone may be maintained in some populations through nongenetic, environmental factors rather than genetic constraints.
A Regio‐ and Stereoselective Ring‐Opening Polymerization Approach to Isotactic Alternating Poly(lactic‐co‐glycolic acid) with Stereocomplexation
Authors: Huang Yu‐Ting , Huang Hao‐Yi , Cheng Jing‐Liang , Xie Min , Feng Liang‐Wen , Cai Zhongzheng , Zhu Jian‐Bo 
Published: January 2025

AbstractPoly(lactic‐co‐glycolic acid) (PLGA) has been widely employed for various biomedical applications owing to its biodegradability and biocompatibility. The discovery of the stereocomplex formation between enantiomeric alternating PLGA pairs underscored its potential as high‐performance biodegradable materials with diverse material properties and biodegradability. Herein, we have established a regio‐ and stereoselective ring‐opening polymerization approach for the synthesis of stereocomplexed isoenriched alternating PLGA from racemic methyl‐glycolide (rac‐MG). The high sequence and tacticity control was accomplished by an optimized enantiopure scandium catalyst bearing a spiro‐salen scaffold. Varying polymer stereoregularity Pm from 0.4 to 0.91 led to a transformation of the resulting alternating PLGA from amorphous to semicrystalline materials. Notably, the stereocomplexed alternating PLGA demonstrated enhanced melting transition temperature (Tm up to 191 °C) and crystallization rate. This regio‐ and stereocontrolled polymerization represented a versatile approach for the preparation of high‐performance biodegradable PLGA materials.
Total Syntheses of Bryostatins 1, 7, 9 and 9‐N3
Authors: Guo Qianyou , Yang Yufan , Zhang Hongyun , Wang Dongxu , Li Bing , Jiang Dongyang , Tu Xiaoyu , Gao Xin , Zhang Chao , Qin Yong , Gao Lu , Wang Wanshu , Song Zhenlei 
Published: January 2025

AbstractBryostatins are a family of marine natural products that have garnered significant interests, as evidenced by over 40 clinical trials. However, their extremely low natural abundance has severely limited further research. Despite significant efforts, which have led to the total synthesis of seven bryostatin members by eight independent research groups, these complex molecules present persistent challenges for stereocontrolled, large‐scale, and especially divergent synthesis. Here, we report the divergent total syntheses of bryostatins 1, 7, 9 and 9‐N3. Notably, 1.5 g of bryostatin 1 was obtained in a single sequence of the final three steps. Key transformations include Ni‐catalyzed reductive cross‐coupling to rapidly assemble the northern fragment, the flow chemistry‐assisted visible light‐induced alkyl radical conjugate addition to convergently construct the southern fragment, and an intramolecular geminal bis(silyl) Prins cyclization to establish the cis‐Z selectivity on the B‐ring. The syntheses consist of 20–22 steps in the longest linear sequence and 33–35 total steps, with overall yields ranging from 3.3 % to 4.5 %.
A Luminescent Lanthanide MOF for the Selective and Ultra‐High Sensitivity Detection of Caffeic Acid
Authors: Li Yufen , Wang Xiaomei , Tan Zhenqi , Liu Lanqing , Wang Qicheng , Yang Tao , Zhou Xinhui , Niu Zhigang 
Published: January 2025

ABSTRACTA novel metal–organic framework (MOF) [Eu3L4(CH3COO) (H2O)7]·(CH3CN)5 (1) based on the ligand H2L (3′,5′‐di(1H‐1,2,4‐triazole‐1‐yl)‐[1,1′‐biphenyl]‐3,5‐dicarboxylic acid) has been synthesized by the hydrothermal method. A complete characterization of 1 was conducted, employing a range of techniques including crystallography, PXRD, SEM, FTIR spectroscopy, and TGA analysis. The fluorescence sensing behavior of 1 was examined in a solution of N,N‐dimethylformamide (DMF). The results demonstrated that 1 exhibited a pronounced “turn‐off” fluorescence phenomenon in the presence of caffeic acid (CA). Despite the interference of 11 potential substances, 1 was still capable of effectively recognizing CA. The luminescence intensity ratio of 1 showed a strong linear relationship with CA concentration in the range 0–5 μM. The Ksv value was 2.49 × 105 M−1. Additionally, the detection mechanism was studied using PXRD, SEM, UV–Vis, and fluorescence lifetime methods.
Effect of CoFe2O4 and NiFe2O4 Nanoparticles on Dielectric and Piezoelectric Properties of Poly(Vinylidene Fluoride) Nanocomposite Films
Authors: Parangusan Hemalatha , Karuppasamy K. , Saboor Abdul , Hamyasa Yousef Mohammad Nazarul , Alfantazi Akram , Bhadra Jolly 
Published: January 2025

ABSTRACTOur research ventures into the realm of flexible piezoelectric nanogenerators (PENGs), a field brimming with potential for harvesting sustainable energy from diverse sources. We have taken a novel approach, developing a highly flexible PENG by harnessing the power of polyvinylidene fluoride (PVDF) and its nanocomposites. The β‐phase content and crystallinity of the PVDF‐based composite films are meticulously evaluated using Fourier transformation infrared spectroscopy (FTIR) and X‐ray diffraction (XRD). The peak‐to‐peak output voltage of pure PVDF, PVDF/CoFe2O4, and PVDF/NiFe2O4 composite‐based PENGs are 101 mV, 2.47 V, and 1.9 V, respectively. The enhanced output efficiency of the flexible PENG is a direct result of the electroactive polar phases produced in the PVDF when the nanomaterials are incorporated. This pioneering work underscores the potential of a PVDF/CoFe2O4 and PVDF/NiFe2O4 composite film‐based PENG as a feasible energy harvester for wearable and portable devices.
Delayed Fluorescence in Dual‐Doped Perovskite Nanocrystals: Insight into the Role of Dopants
Authors: Mandal Prasenjit , Panja Soumya , Viswanatha Ranjani 
Published: January 2025

AbstractThis study investigates the photophysical behaviour of Mn/Fe and Mn/Sn co‐doped CsPbCl3 perovskite nanocrystals (NCs) to explore carrier dynamics and dopant interactions. Using gated photoluminescence (PL) and temperature‐dependent measurements, we elucidate the impact of dopant chemistry on exciton behaviour, focusing on vibrationally assisted delayed fluorescence (VADF) and energy transfer mechanisms. The efficiency of VADF is influenced by factors such as the bandgap, temperature, quantum confinement, and host composition. In Mn/Fe co‐doped NCs, Fe‐induced trap states introduce additional radiative pathways, resulting in a unique emission around 515 nm. In contrast, in Mn/Sn co‐doped NCs, Sn captures photoexcited carriers, preventing the VADF process and leading to non‐radiative decay. The findings provide key insights into dopant‐dopant interactions and the mechanisms governing carrier transfer, enhancing our understanding of these materials for future optoelectronic applications.
Emerging Role of Noncovalent Interactions and Disulfide Bond Formation in the Cellular Uptake of Small Molecules and Proteins
Authors: Jakka Surendar R. , Mugesh Govindasamy 
Published: January 2025

AbstractIntracellular delivery of proteins and small molecules is an important barrier in the development of strategies to deliver functional proteins and therapeutics into the cells to realize their full potential in biotechnology, biomedicine, cell‐based therapies, and gene editing protein systems. Most of the intracellular protein delivery strategies involve the conjugation of cell penetrating peptides to enable the permeability of plasma membrane of mammalian cells to allow proteins to enter cytosol. The conjugations of small molecules such as (p‐methylphenyl) glycine, pyrenebutyrate and cysteines are used for the same purpose. Molecular level interactions are governed mostly by ionic (cationic/anionic), covalent and noncovalent interactions with various molecular entities of glycocalyx matrix on plasma membrane lipid bilayer. Although the role of noncovalent interactions in cellular uptake is not fully understood, several recent advances have focused on the noncovalent interaction‐based strategies of intracellular delivery of small molecules and proteins into mammalian cells. These are achieved by simple modification of protein surfaces with chemical moieties which can form noncovalent interactions other than hydrogen bonding. In this review, we describe the recent advances and the mechanistic aspects of intracellular delivery and role of noncovalent interactions in the cellular uptake of proteins and small molecules.
Discrete‐time high‐order fully actuated robust stabilization control for a type of combined spacecraft subject to uncertainties
Authors: Cui Kai‐Xin , Duan Guang‐Ren , Cui Yang 
Published: January 2025

AbstractThis paper investigates discrete‐time high‐order fully actuated (DT‐HOFA) robust stabilization control for a type of combined spacecraft simulator (CSS) subject to nonlinear uncertainties. First, a discrete‐time second‐order fully actuated system (FAS) model is developed for the CSS system and generalized to a general DT‐HOFA system (HOFAS) with nonlinear uncertainty, after which the robust control objective is proposed. Then, based on a linear matrix inequality condition, a robust stabilizing controller for the uncertain DT‐HOFAS is designed under certain nonlinear uncertainties, which produces a closed‐loop system with arbitrarily assignable eigenstructure. The assignable nature of the closed‐loop system is further utilized to improve the system performance by solving a multi‐objective optimization problem. Finally, the proposed approach is applied successfully to solve the robust stabilization control task of CSS with nonlinear uncertainties, and simulation and experimental results verify its convenience and effectiveness for practical use.
Charakterisierung Digitaler Brückenzwillinge zur Integration messwertgestützter Tragsicherheitsnachweise
Authors: Esser Pauline , Walker Maria , Lazoglu Alex , Ulbrich Lisa , Mosig Oliver , Marx Steffen 
Published: January 2025

AbstractMit dem Zukunftsbild Digitaler Zwilling visioniert der Masterplan BIM Bundesfernstraßen des BMDV die digitale Repräsentation und Vernetzung aller bauwerksrelevanten Daten der realen Infrastruktur. Insbesondere für Brücken als neuralgische Bestandteile der Verkehrsinfrastruktur ermöglichen Digitale Zwillinge eine optimierte Zustandsbewertung. Die erfolgreiche Einbindung Digitaler Brückenzwillinge in das Erhaltungsmanagement erfordert standardisierte Konzepte für deren Entwicklung. Hier knüpft das vom BMDV geförderte Forschungsprojekt ANYTWIN mit dem Ziel an, messwertgestützte Tragsicherheitsnachweise in Digitale Brückenzwillinge einzubinden. Die Konzeptentwicklung umfasst eine umfangreiche, branchenübergreifende Recherche vorhandener Definitionen und Charakteristika. Es wurden reifegradabhängige Merkmale Digitaler Brückenzwillinge herausgearbeitet und deren Einfluss auf die messwertgestützte Nachweisführung untersucht. Abschließend wurden BPMN‐Prozessdiagramme als anschauliches Werkzeug verwendet, um den Einfluss jeweiliger Reifegrade auf Prozessebene zu beschreiben.
Mechanisms of Lipid‐Associated Macrophage Accrual in Metabolically Stressed Adipose Tissue
Authors: Reinisch Isabel , Enzenhofer Sarah , Prokesch Andreas 
Published: January 2025

ABSTRACTAdipose tissue (AT) inflammation, a hallmark of the metabolic syndrome, is triggered by overburdened adipocytes sending out immune cell recruitment signals during obesity development. An AT immune landscape persistent throughout weight loss and regain constitutes an immune‐obesogenic memory that hinders long‐term weight loss management. Lipid‐associated macrophages (LAMs) are emerging as major players in diseased, inflamed metabolic tissues and may be key contributors to an obesogenic memory in AT. Our previous study found that LAM abundance increases with weight loss via intermittent fasting (IF) in obese mice, which is driven by adipocyte p53 signalling. However, the specific signals causing LAM accumulation in AT under IF remain unknown. In this piece, we hypothesise on a range of adipocyte‐secreted signals that can harbor immune‐attractive features upon fasting/refeeding cycles. We highlight possible mechanisms including cell death signalling, matrikines, and other damage‐associated molecular patterns (DAMPs), as well as adipo(‐cyto)kines, lipid mediators, metabolites, extracellular vesicles, and epigenetic rewiring. Finally, we consider how advances in mechanisms of AT LAM recruitment gleaned from preclinical models might be translatable to long‐term weight management in humans. Thus, we provide vantage points to study signals driving monocyte recruitment, polarisation towards LAMs, and LAM retention, to harness the therapeutic potential of modulating AT LAM levels by impacting the immune‐obesogenic memory in metabolic disease.
Safety and Efficacy of Nusinersen Focusing on Renal and Hematological Parameters in Spinal Muscular Atrophy
Authors: Bahadır Şenol Hüseyin , Yıldız Gizem , Polat Ayşe İpek , Aydın Adem , Hız Ayşe Semra , Soylu Alper , Yiş Uluç 
Published: January 2025

ABSTRACTBackgroundSpinal muscular atrophy (SMA) is a motor neuron disease caused by mutations in the SMN1 gene. Nusinersen, an antisense oligonucleotide, has been shown to improve motor function in SMA patients. However, concerns regarding its renal safety remain as previous studies have linked similar treatments to renal toxicity.ObjectiveThe aim of this study was to evaluate the effects of the nusinersen treatment on platelet counts and renal functions, specifically urine protein excretion, in SMA patients and to estimate safe urinary protein levels before administration of each intrathecal injection.MethodsThis retrospective study examined data from 33 patients with SMA to assess the effects of nusinersen on motor functions and laboratory parameters including platelet count, serum creatinine, urine protein, and urine creatinine. Measurements were taken at baseline andprior to each maintenance dose, after the completion of four initial loading doses. The baseline values were compared between SMA Type 1 and Type 2 patients, while the changes in these values over time were analyzed within each group.ResultsNo significant adverse effects on platelet counts or renal functions were observed. Urine creatinine and protein levels were significantly higher in SMA Type 2 patients compared to SMA Type 1 at baseline; these parameters remained stable in SMA Type 2 but increased significantly after the loading doses in SMA Type 1. Motor function improvements were observed in both groups, with the most significant gains in SMA Type 1 after the loading doses. Thus, improvement in motor functions was associated with increase in urine creatinine.ConclusionNusinersen treatment did not cause significant renal toxicity or affect platelet counts. Urine creatinine levels may serve as a potential biomarker for assessing treatment response in SMA Type 1.
Effects of Quetiapine on Novelty‐Related Object Recognition Memory and Hippocampal BDNF Level in Sleep‐Deprived Rats
Authors: Özcan Öznur Özge , Çevreli Burcu , Kaşıkçı Emel Serdaroğlu , Karahan Mesut , Konuk Muhsin 
Published: January 2025

ABSTRACTBackgroundThe underlying mechanism of quetiapine (QET) in treating cognitive impairment in sleep deprivation is unclear. The present study aimed to evaluate the effects of treatment with QET on novel object recognition and hippocampal (hippo) brain‐derived neurotrophic factor (BDNF) levels in rats submitted to 72 h sleep deprivation (SD).Materials and MethodsA total of 42 adult male Wistar albino rats were assigned into six experimental groups: non‐sleep‐deprived (NSD) control, short‐term control group (n = 7) received a single intraperitoneal (i.p.) injection 10 mg/kg QET of 1 mL saline (4 days) (NSD‐STQET), long‐term control group (n = 7) received single i.p. injection 10 mg/kg QET of 1 mL saline (30 days) (NSD‐LTQET); 72 h sleep‐deprived (SD) group, 72 h SD short‐term group received short‐term i.p. injection 10 mg/kg QET of either (n = 7) (SD‐STQET), and 72 h SD long‐term group received long‐term i.p. injection 10 mg/kg QET of either (n = 7) QET (SD‐LTQET). SD was performed using the modified multiple‐platform technique in a water tank for 72 h. Additionally, we aim to reveal the consequences of 72 h SD and QET effects on memory processes with hippo BDNF levels by testing rats in the novel object recognition (NOR) test and ELISA method.ResultsLong‐term QET administration in healthy rats decreased NOR and BDNF protein expression in the hippocampus, as did 72 h SD. Long‐ and short‐term QET administration reversed SD effects, but only short‐term QET administration increased hippo BDNF.ConclusionThese results suggest that the beneficial effects of QET on SD may be partly related to the upregulation of recognition memory and neuroprotective proteins such as BDNF. However, long‐term QET treatment in the absence of a disease model may have the potential to negatively impact recognition memory and BDNF levels, which support synaptic plasticity and cognitive function.
Identification of Critical Signature in Post‐Traumatic Stress Disorder Using Bioinformatics Analysis and in Vitro Analyses
Authors: Liu Lifen , Liu Yang , Li Rui , Teng Yue , Zhao Shuyang , Chen Jinhong , Li Changjiang , Hu Xinyu , Sun Lin 
Published: January 2025

ABSTRACTBackgroundPost‐traumatic stress disorder (PTSD) is a complex psychiatric condition that emerges following exposure to trauma and significantly affects daily functioning. Current research is focused on identifying effective treatments for PTSD. Advances in bioinformatics provide opportunities to elucidate the underlying mechanisms of PTSD.MethodsRNA sequencing (RNA‐seq) datasets were retrieved from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using GEO2R. Weighted gene co‐expression network analysis (WGCNA) was employed to examine gene correlation patterns. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed for functional annotation and enrichment analysis, respectively. The MCODE plugin in Cytoscape software was utilized to analyze the protein–protein interaction (PPI) network. Anxiety and depression in a mice stress model were assessed using the open‐field test (OFT), elevated plus maze test (EPMT), and forced swimming test (FST). Real‐time quantitative PCR (qRT‐PCR) was conducted to validate key genes in stress‐exposed models.ResultsA total of 157 common upregulated DEGs and 53 common downregulated DEGs were identified in the amygdala (AMY) and the hippocampus (HIP). Notably enriched pathways included neuroactive ligand‐receptor interaction, mechanistic target of rapamycin (mTOR) signaling pathway, nicotine addiction, and dopaminergic synapse. The PPI network identified four hub genes, with key pathways associated with nicotine addiction and dopaminergic synapse. qRT‐PCR validation confirmed that the expression trends of these four genes were consistent with microarray data. Behavioral tests (OFT, EMPT, and FST) revealed significant changes.ConclusionThis study utilized bioinformatics and in vitro experiments to identify genes and pathways potentially crucial for PTSD development. Key genes were validated in a mouse model, providing insights into potential target genes for PTSD treatment.