bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiomics analysis revealed miRNAs as potential regulators of the immune response in Carassius auratus gills to Aeromonas hydrophila infection
Authors: Huo Jiaxin , Hu Xiucai , Bai Jie , Lv Aijun 
Published: February 2023

The gill of fish is an important immune organ for pathogen defense, but its microRNA (miRNA) expression and regulatory mechanism remain unclear. In this study, we report on the histopathological and immunohistochemical features of the gills of the crucian carp Carassius auratus challenged with Aeromonas hydrophila. Small RNA libraries of the gills were constructed and sequenced on the Illumina HiSeq 2000 platform. A total of 1,165 differentially expressed miRNAs (DEMs) were identified in gills, of which 539 known and 7 unknown DEMs were significantly screened (p &lt; 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the potential target genes/proteins were primarily involved in 33 immune-related pathways, in which the inflammatory responses were focused on the Toll-like receptor (TLR), mitogen-activated protein kinase (MAPK), and nuclear factor kappa B (NF-κB) signaling pathways. Moreover, the expression levels of 14 key miRNAs (e.g., miR-10, miR-17, miR-26a, miR-144, miR-145, and miR-146a) and their target genes (e.g., TNFα, TLR4, NF-κB, TAB1, PI3K, and IRAK1) were verified. In addition, the protein levels based on isobaric tags for relative and absolute quantification (iTRAQ) were significantly associated with the results of the quantitative real-time PCR (qRT-PCR) analysis (p &lt; 0.01). miR-17/pre-miR-17 were identified in the regulation expression of the NF-κB target gene, and the phylogenetic tree analysis showed that the pre-miR-17 of C. auratus with the closest similarity to the zebrafish Danio rerio is highly conserved in teleosts. This is the first report of the multi-omics analysis of the miRNAs and proteins in the gills of C. auratus infected with A. hydrophila, thus enriching knowledge on the regulation mechanism of the local immune response in Cyprinidae fish.
Plano de intervenção: implantação de práticas educacionais para melhor adesão à campanha de vacinação contra HPV em uma unidade básica de saúde da família
Authors: Da Silva Jéssyca Rodrigues , Carvalho João Victor Sousa , Da Silva Joissilane Rodrigues , Lima Rayza Gregório , Lacerda Talita Oliveira do Vale , Ribeiro Natasha Cortez Silva 
Published: February 2023

O Papiloma Vírus Humano (HPV) é uma Infecção Sexualmente Transmissível (IST) causada por um vírus de DNA, da família papilomaviridade, que tem um notório potencial oncológico, em alguns de seus subtipos, principalmente nos de alto risco: 16, 18, 31, 33, 35, 39, 45, 51, 52,56, 58, 59, 68, 73 e 82. Dessa maneira, fica evidente que a prevenção da infecção por HPV é uma forma estratégica de controle dessa IST de grande potencial cancerígeno e considerável prevalência. Trata-se de um estudo de intervenção na comunidade (projeto de intervenção) que tem por finalidade; reduzir a evasão e aumentar a adesão dos pacientes jovens atendidos na Unidade Básica de Saúde Jotta a campanha de vacinação contra HPV, estabelecendo, para tanto, estratégias de educação, incentivo e esclarecimento. De acordo com o proposto, foram realizadas as ações planejadas na pesquisa de forma ordenada no período de outubro a dezembro, na Unidade Básica de Saúde Jotta, para alunos jovens, de ambos os sexos, na faixa etária entre 9 e 14 anos acompanhados, com a finalidade de mensurar e capacitar acerca da importância da aceitação da vacina anti-HPV. As intervenções foram feitas quinzenalmente, totalizando 5 palestras de 16 outubro à 11 dezembro (16 e 30 de outubro; 13 e 27 de novembro; 11 de dezembro). Ao que tange os dados levantados e tabulados, observa-se que sobre o domínio de conhecimentos, a questão que obteve o maior número de acertos antes da aplicação da intervenção foi a de número 3, que indagava sobre o HPV ser uma doença sexualmente transmissível; já a que apresentou o maior percentual de acertos após a intervenção continuou sendo a questão 3; acompanhando esta, a questão 1 também teve o mesmo quantidade de respostas corretas após a atividade da intervenção, na qual perguntava a respeito do que é o HPV. Em posse desses dados estatísticos, tornou-se viável a análise do impacto das palestras sobre o público-alvo, a fim de identificar se a aula expositiva com slides é uma prática educacional eficiente em vista de uma melhor adesão da vacina do HPV. Observou-se que o conhecimento geral entre os adolescentes que constituem o público-alvo, em respeito à origem viral do HPV, suas repercussões clínicas e a importância de sua profilaxia se demonstra aquém do esperado, o que representa uma significativa barreira contra a vacina. Elucidou-se resultados semelhantes a outras pesquisas realizadas com a mesma temática, evidenciando um nível de conhecimento aquém do esperado entre o público-alvo, tanto no que tange ao vírus quanto a sua vacina.
Onde estão os livros… a biblioteca nas nuvens?!
Authors: Rodrigues Maria Eduarda Pereira , Pulido Margarita Pérez , Guerrero António Pulgarin 
Published: February 2023

Na atualidade, o acesso à informação parece ser quase tão valioso quanto a própria informação. Ao longo dos anos, o papel das bibliotecas era geralmente caracterizado como incluindo o desenvolvimento de coleções, a catalogação e indexação de livros, revistas e outros materiais, e os serviços ao utilizador, nomeadamente o serviço de empréstimo. No entanto, naturalmente com o tempo essas funções foram-se modificando sendo acrescidas de outras mais focadas na informação e menos no documento. Assim, para além dessas funções, as bibliotecas passaram a ser facilitadoras do acesso à informação. Os utilizadores das bibliotecas do ensino superior podem apresentar diferentes graus de proficiência na forma como lidam e acedem tanto à informação como aos documentos disponíveis nas bibliotecas das suas instituições de ensino superior. Muitas vezes, isso pode estar relacionado com a maneira como as bibliotecas são capazes de cumprir seu papel, designadamente de partilha de informação sobre os recursos existentes na biblioteca e sobre a sua utilização. Tendo em conta este contexto, o tema da apresentação desta conferência é biblioteca da ESACB e os diferentes recursos informacionais e serviços disponíveis para os seus utilizadores. Isso inclui: as características dos recursos da biblioteca; como esses recursos funcionam, ou seja, a base de dados de resumos e citações SCOPUS, que é o recurso mais recente disponível na biblioteca; a plataforma b-on (i.e. Biblioteca de conhecimento online); Recursos de acesso aberto e tutoriais disponíveis na página da biblioteca.
A atividade de inteligência de segurança publica na visão do Superior Tribunal de Justiça – análise do HC Nº 512.290
Authors: Bail Omar , Rocha João Pedro Passos 
Published: February 2023

No final dos anos de 1990 a Atividade de Inteligência no Brasil foi reestruturada. Uma série de instrumentos normativos estabeleceram sua feição formal. Houve a especialização da atividade, sendo formatada a Inteligência de Segurança Pública. A atividade consubstanciou-se em importância para os órgãos policiais no art. 144 da Constituição Federal, notadamente em sua natureza executiva, de efetivo combate ao crime, em consonância com a atribuição desses órgãos, fornecido pelo texto constitucional. O emprego do conhecimento adquirido pela atividade de inteligência passou a auxiliar o Ministério Público em subsídio a sua atuação no processo. Contestações surgiram, alegando a ilicitude das provas produzidas. Analisando um caso específico, o Habeas Corpus nº 512.290 – RJ, buscou-se conhecer a visão do Superior Tribunal de Justiça sobre o tema em face da relevância deste Tribunal na estrutura do Poder Judiciário. As conclusões extraídas possibilitam definir o rumo da Atividade de Inteligência de Segurança Pública para os órgãos policiais, em especial à Polícia Militar do Paraná, em face de sua bem delineada Estratégia de Inteligência.
Geographic Information System Mapping of Plantation
Authors: Pramuditha Shinta Dewi Puspitasari  , Agus Kurniasari Arvita , Trismayanti Dwi Puspitasari  
Published: February 2023

Perusahaan Daerah Perkebunan (PDP) Kahyangan is a Regional Owned Enterprise in Jember Regency which was formed to realize the prosperity of the community. In Regional Regulation Number 2 of 2012-chapter 3 clauses 2, the government can manage regional finances by exploring sources of Regional Original Income, one of which is from the PDP. In addition, the purpose of the establishment of the PDP is as a means of developing the economy in the context of regional development by increasing productivity from all aspects to increase regional income. The main commodities are coffee and rubber, cloves, Mahoni, and Sengon wood are supporting commodities. Based on interviews PDP's profit contribution to Jember's income has continued to decline in recent years due to the absence of good management of its potential. So, this research will manage a system that can assist in obtaining information about the distribution of plantation land commodities in Jember, as well as mapping existing commodities by Geographic Information System (GIS). The method for developing GIS uses a Design Sprint. The output of this method prototypes and will implement in the GIS application.
Antioxidant Capacity Test, Total Phenolic, Total Alkaloid, and Toxicity of Marigold Flower (Tagetes Erecta L.)
Authors: Tenggono Son Hadinata , Limanan David 
Published: February 2023

Antioxidants are a set of compounds that can fight the effects of reactive oxygen species (ROS) so as to eliminate their effects in the form of damage to macromolecules of carbohydrates, fats, proteins, and DNA chains. One source of antioxidants that are easily available and widely available is through herbal ingredients such as marigold flowers (Tagetes erecta L.). Marigold flowers are well known and used as a source of antioxidants, anti-inflammatory, antibacterial, diuretic and accelerate wound healing. This flower is often consumed as an edible flower and is also drunk in the form of tea. This study aims to look for the antioxidant capacity, total phenolic, total alkaloids, and toxicity of marigold flowers. Marigold flower extract is obtained by evaporating the results of methanol maceration from dried marigold flowers. Test antioxidant capacity with DPPH (2,2-diphenyl-1-picrilhydrazyl) using Blois method, total phenolic using Singleton and Rossi method, total alkaloid by method by Trivedi et al, and BSLT toxicity by Meyer method. The antioxidant capacity of marigold flowers is measured in IC50 as much as 74.5 μg/mL which includes active antioxidant levels. The total phenolic content of marigold flowers was obtained as much as 10,350.68 μg/mL. Total alkaloid levels were obtained as much as 13.05 μg/mL. The BSLT toxicity test in LC50 is 162.82 μg/mL which is categorized as moderate toxicity. From the results of this study, marigold flowers can be used as a source of antioxidants and have antimitotic properties.
Reverse The Name of The Certificate of Title To The Estate Unilaterally Without The Knowledge of The Other Heirs
Authors: Aprilian Herdiana Nabilla Rizki , Neltje Saly Jeane 
Published: February 2023

Property Rights are the descending rights, strongest and fullest that a person can have over land. Property rights can be transferred and transferred to other parties because a legal event occurs due to the death of a person so that the property left behind passes to the heirs and legal actions occur if there is a transfer of rights from the rights holder or his heirs to the new rights holder. If there is an abandoned land certificate, the land certificate is also transferred. A certificate is a proof of land rights to guarantee legal certainty. How is the legal settlement of the reverse action of the title certificate to the estate unilaterally without the knowledge of the other heirs. The research method used in writing tesis in the form of a normative juridical approach method. Specifications The research used in this study is analytical descriptive research. The types and sources of data used by the author are secondary data types, data obtained through data sources of civil law literature studies and primary legal materials, secondary legal materials, tertiary legal materials. Data collection techniques are obtained through literature studies and interviews. The method of analysis in thesis research is in the form of Qualitative Juridical. The results of the research on the reverse name of the title certificate to the heirs unilaterally without the knowledge of other heirs; The first provides legal protection for heirs who have full rights related to the act of unilaterally reversing the name of the title certificate to the estate without the knowledge of the other heirs is that each heir has the right to file a lawsuit through the Court to fight for his heir rights as per Article 834 of the Civil Code. Secondly, the legal settlement of the reverse action of the name of the title certificate to the estate unilaterally without the knowledge of the other heirs is to file a lawsuit through the court institution if the court decision has been issued that has obtained permanent legal force, the title certificate contains defects law in its issuance and declared invalid and unlawful
Safety Implementation Analysis and Occupational Health at Kretek 2 Bridge Work In Bantul Regency
Authors: Nashrullah Arifin Isyraq , Oetomo Wateno , Djoko Nugroho Laksono 
Published: February 2023

Construction work includes the implementation of construction work and the community organizing the construction work itself. The implementation of this construction work must comply with the provisions regarding engineering, occupational safety and health, labor protection, and local environmental regulations to ensure the realization of an orderly implementation of construction work. The construction of the Kretek 2 Bridge in Bantul Regency, Special Region of Yogyakarta Province has its own challenges, because the location of the bridge is in an area prone to earthquakes and liquefaction or ground movement. With so many workers on this very high-risk project, there is a high probability of an accident occurring. The purpose of the study was to analyze (1) the risky work that could occur in bridge construction work, (2) the work that has the highest possible risk, (3) risk control, (4) the amount of investment costs required. The method used is the HIRADC method. The results of the study were (1) Risky work that can occur was a. High level of risk, including drowning, being dragged by the river current, being hit by heavy equipment maneuvers and falling from a height. b. Moderate risk level, including being crushed by formwork, falling material, scratched hands. c. Low level of risk, including landslides, heavy equipment maneuvers, iron puncture. (2) Work that has the highest possible risk is Pile Cap Excavation Work, Work Floor Work and Pier Head Ironing Work. (3) Risk control in bridge work consists of: Using a boat when measuring in rivers, Conducting briefings before starting activities, Using PPE life vests and placing life ring buoys on boats, Installing signs and placing officers in areas that intersect with roads, Installing guardrail of the project area, installing scaffolding as a working platform and inspecting its feasibility, communicating hazards through signs, conducting refresh training for workers. (4) The investment cost required for implementing K3 on the Kretek 2 Bridge work in Bantul Regency was Rp. 6,545,998,310 or 1.795% of the contract value.
PEMBIAYAAN PELAYANAN PERSALINAN BAYI LAHIR SEHAT BERDASARKAN PERATURAN DIREKTUR BPJS YANG DIBATALKAN OLEH PUTUSAN MAHKAMAH AGUNG
Authors: Arifin Samsul 
Published: February 2023

Penelitian berjudul “Pembiayaan Pelayanan Persalinan Bayi Lahir Sehat Berpedoman Peraturan Direktur BPJS Yang Dibatalkan Oleh Putusan Mahkamah Agung”, dengan membahas permasalahan: Apa ratio-legis diterbitkannya Perdirjampelkes No. 03 tahun 2018 tentang penjaminan pelayanan persalinan dengan bayi baru lahir sehat dalam program Jamkes dan apa akibat hukum dibatalkannya peraturan direktur BPJS No. 3 / 2018 oleh Putusan MA Nomor 58P/HUM/ 2018. Metode yang digunakan dalam penelitian ini yuridis normatif, hasil penelitian disimpulkan sebagai berikut: Ratio-legis terbitnya Perdirjampelkes No. 03 tahun 2018, untuk pengembangan sistem pembayaran pelayanan kesehatan, diimplementasikan pembayaran kepada Faskes secara efektif dan efisien, hanya memberikan manfaat pelayanan kesehatan yang bermutu dengan memperhatikan sustainabilitas program JKN KIS. BPJS Kesehatan menjamin pelayanan persalinan dan bayi baru lahir, dengan kondisi sehat yang mendapatkan pelayanan neonatal esensial dan tidak memerlukan perbuatan merawat dengan sumber daya khusus, baik dilahirkan melalui perbuatan bedah caesar maupun persalinan pervaginam, dengan penyulit atau tanpa penyulit, dibayar dalam satu paket persalinan. BPJS tidak menjamin untuk bayi lahir yang memerlukan perbuatan merawat dengan sumber daya khusus, dibayar terpisah dari paket persalinan. Akibat hukum dibatalkannya peraturan direktur BPJS No. 3/2018 oleh Putusan MA Nomor 58P/HUM/ 2018, maka Dirjampelkes tidak mempunyai wewenang menerbitkan Perdirjampelkes Nomor 3 Tahun 2018 oleh putusan MA Nomor 58 P/HUM/ 2018, karena itu tanpa dibatalkan oleh MA atas dasar uji materi, peraturan tersebut tidak mempunyai kekuatan mengikat untuk dilaksanakan dikarenakan melanggar aturan Undang – Undang Dasar dan aturan perundang – undangan, maka terkait dengan Penjaminan Pelayanan Persalinan tentang Bayi Baru Lahir Sehat dalam Program Jamkes dibiayai oleh negara dengan ketentuan menjadi beserta Jamkes.
Penanganan Anak Keterlambatan Berbicara (Speech Delay) Usia 5 - 6 Menggunakan Metode Bercerita Di Indonesia
Authors: Budiarti Erna , Kartini Rima Dewi , Putri H Saniyya , Indrawati Yulia , Daisiu Konny Fransiska 
Published: February 2023

Perkembangan bahasa pada setiap anak pastilah berbeda. Hal ini dipengaruhi oleh stimulus yang diberikan oleh orang terdekat yang ada disekelilingnya. Pada anak usia dini berusia 5-6 tahun, tahapan perkembangan bahasanya dapat kita amati dari kelancaran saat anak berbicara, apakah anak sudah bisa berbicara secara lancar atau belum, serta saat berkomunikasi secara lisan kata kata yang disampaikan juga sudah lebih kompleks, akan tetapi bagi anak dengan keterlambatan bericara dibutuhkan kekhususan dalam melakukan stimulasi, penanganan khusus tersebut dapat dilakukan dengan berbagai cara salah satunya adalah dengan melalui media bercerita sebagaimana yang dilakukan PAUD Aditya Karawang. Penelitian ini menggunakan jenis penelitian deskriptif kualitatif. Adapun sumber data penelitian adalah siswa speech delay, guru, orang tua siswa serta ahli terapis wicara. Penelitian menunjukkan bahwa di PAUD Aditya Karawang terdapat siswa yang memiliki gangguan keterlambatan bicara jenis Speech Delay. Siswa tersebut berusia 4-6 tahun. Faktor yang mempengaruhi keterlambatan berbicara antara lain kecerdasan, penggunaan bahasa kedua, gaya berbicara yang ditirukan oleh anak, kesehatan serta keharmonisan didalam keluarga. Semua faktor tersebut saling berkaitan dan berpengaruh kepada perkembangan bahasa anak. Kami berharap dengan memberikan atau menggunakan metode bercerita kepada anak, anak dapat terlatih untuk menggunakan bahasa yang benar saat berkomunikasi secara lisan.
Penelitian menunjukkan bahwa di PAUD Aditya Karawang terdapat siswa yang memiliki gangguan keterlambatan bicara jenis Speech Delay. Siswa tersebut berusia 4-6 tahun. Faktor yang mempengaruhi keterlambatan berbicara antara lain kecerdasan, penggunaan bahasa kedua, gaya berbicara yang ditirukan oleh anak, kesehatan serta keharmonisan didalam keluarga. Semua faktor tersebut saling berkaitan dan berpengaruh kepada perkembangan bahasa anak. Kami berharap dengan memberikan atau menggunakan metode bercerita kepada anak, anak dapat terlatih untuk menggunakan bahasa yang benar saat berkomunikasi secara lisan.
The Effect Of Mobile Banking Product Quality On Customer Satisfaction Of Indonesian Sharia Bank Jakarta Wolter Monginsidi Branch
Authors: Wiharso Gani , Hendri Prasetyo Johan , Prakoso Bobby Suryo , Fabrianto Luky 
Published: February 2023

Bank is a financial institution that collects funds from the public in the form of deposits, both demand deposits, savings and time deposits. This study aims to determine the effect of mobile banking product quality on customer satisfaction of Bank Syariah Indonesia Jakarta branch Wolter Monginsidi. The method use is a survey methode using descriptive and verification analysis technique. The sampling technique of this study used a purposive sampling technique of 75 respondents. The data collection technique use quisionnaire with 20 question items assessed on a Likert scale 1-5 and tested for validity and reliability. Hyphotesis testing using statistical analysis included classical assumption test (Normality test and Heteroscedasticity test), Autocorrelation test (Dubin Watson), Simple Linier Regression Analysis (R square, t-test, and F test). Based on partial test, it can be stated that Product Quality has a positive effect on visitors to the Jakarta branch of Bank Syariah Indonesia Wolter Monginsidi, so the hypothesis can be accepted.
Penerapan Model Pembelajaran Problem-Based Learning untuk Meningkatkan Hasil Belajar Siswa Pada Materi Himpunan di Kelas VII F MTs Negeri Jepon
Authors: Hayati Yani 
Published: February 2023

Penelitian ini bertujuan untuk mengetahui gambaran penerapan model problem-based learning terhadap peningkatan aktifitas dan hasil belajar siswa pada materi himpunan di kelas VII F MTs Negeri Jepon semester 2 tahun pelajaran 2015/2016. Pelaksanaan penelitian ini menggunakan prosedur penelitian tindakan kelas, yang dilaksanakan melalui dua siklus. Waktu pelaksanaan selama 3 bulan yakni Januari sampai dengan Maret 2016. Teknik pengumpulan data menggunakan dua cara yakni tes digunakan untuk memperoleh data hasil belajar siswa dan non tes untuk mendapatkan data aktifitas siswa selama proses pembelajaran. Hasil penelitian menunjukkan bahwa penerapan model problem-based learning pada materi himpunan dapat meningkatkan aktifitas dan hasil belajar siswa. Besar peningkatan aktifitas siswa 25,77% dari rata-rata 51,31% pada kondisi pra siklus menjadi 77,08% pada kondisi siklus II. Sedangkan besar peningkatan hasil belajar siswa adalah 19,75 dari kondisi pra siklus nilai rata-rata 58,59 ke kondisi siklus II dengan nilai rata-rata 78,34. Perubahan ketuntasan sebesar 50,46% dari kondisi pra siklus tuntas 32,40% ke kondisi siklus II tuntas 82,86%.
ANALISA BUDIDAYA TANAMAN LAHAN SEMPIT: URBAN FARMING DI KECAMATAN MOJOLABAN, KABUPATEN SUKOHARJO
Authors: Prasetyo Agung , Suswadi  , Mahananto  , Prasetyowati Kusriani , Irawan Norbertus Citra , Supriyadi Teguh , Aulia Nadian Nur , Mustaqim  
Published: February 2023

Program pengabdian dilakukan dengan mengadakan kegiatan untuk membantu mengembangkan dan meningkatkan kegiatan budidaya hortikultura dilahan sempit Desa Triyagan, Kecamatan Mojolaban, Kabupaten Sukoharjo. Metode yang dipakai guna mencapai tujuan yang dimaksud adalah penyuluhan, pelatihan dan analisa ekonomi budidaya pertanian lahan sempit. Hasil menunjukan pelatihan budidaya pertanian dilahan sempit didaerah perkotaan dapat dilakukan untuk memenuhi kebutuhan pangan rumah tangga dan menghemat pengeluran pangan sehingga menigkatkan ketahanan pangan rumah tangga. Mitra dapat langsung mempraktekan budidaya tanaman dirumah masing-masing. Besar harapan penulis bahwa dengan menggalakan budidaya disekitar pekarangan rumah diharapkan mampu meningkatkan ketahanan pangan keluarga.