bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential neural circuit vulnerability to β-amyloid and tau pathologies in novel Alzheimer’s disease mice
Authors: Capilla-López Maria Dolores , Deprada Angel , Andrade-Talavera Yuniesky , Martínez-Gallego Irene , Coatl-Cuaya Heriberto , Rodríguez-Alvarez José , Rodríguez-Moreno Antonio , Parra-Damas Arnaldo , Saura Carlos A. 
Published: April 2023

AbstractAlzheimer’s disease (AD) progresses with memory loss and neuropsychiatric symptoms associated with cell specific vulnerability in memory- and emotion-related neural circuits. Neuropathological and synaptic changes are key factors influencing the clinical progression to dementia, but how they cooperate to cause memory and emotional disturbances is largely unknown. Here, we employed pathological, behavioral, expansion microscopy, electrophysiology and transcriptomic approaches to evaluate the effects of amyloid-β (Aβ) and tau on neuropathological progression, synaptic function, and memory and emotional symptoms in amyloid precursor protein (APP), Tau and double novel APP/Tau transgenic mice expressing the mutant human amyloid precursor protein (APPSw,Ind) and/or microtubule-associated protein tau (MAPT) in excitatory neurons. APP/Tau mice of both sexes show spatial learning and memory deficits associated with synaptic tau accumulation and reduced synaptic proteins and neurotransmission in the hippocampus. By contrast, male and female APP/Tau mice exhibit innate anxious behavior and impaired fear memory extinction linked to Aβ pathology and with absence of synaptic tau in the basolateral amygdala (BLA). Intriguingly, APP/Tau mice show NMDA-dependent long-term potentiation (LTP) deficits in the hippocampus but not in the amygdala. Bulk RNA sequencing reveals region-specific but also common transcriptional changes in response to Aβ/tau pathology, including downregulation of synapse transmission and ion channel activity genes. Importantly, we detected 65 orthologs of human AD risk genes identified in GWAS (e.g.,APOE,BIN1,CD33,CLU,PICALM,PLCG2,PTK2B,TREM2,SORL1,USP6NL) differentially expressed in the hippocampus and/or BLA of APP/Tau mice, indicating that this APP/Tau model exhibits transcriptional alterations linked to known molecular determinants of AD development. In conclusion, simultaneous development of Aβ and tau neuropathologies in this double APP/Tau transgenic mouse model reproduces synaptic, behavioral, and molecular alterations associated with AD pathophysiology in a region-specific manner. Our findings highlight region-specific pathological effects of Aβ and tau in excitatory neuronal circuits mediating emotional and memory processing, providing evidence that both factors and their molecular cascades should be considered in future AD preventive and therapeutic strategies.Graphical abstractAge-dependent vulnerability of memory and emotional neural circuits in response to tau and Aβ pathologies.
To let go or not to let go: how ParA can impact the release of the chromosomal anchoring inCaulobacter crescentus
Authors: Puentes-Rodriguez Stephanie G. , Norcross J.D. , Mera Paola E. 
Published: April 2023

ABSTRACTChromosomal maintenance is vital for the survival of bacteria. InCaulobacter crescentus, chromosome replication initiates atoriand segregation is delayed until the nearby centromere-like regionparSis replicated. Our understanding of how this sequence of events is regulated remains limited. The segregation ofparShas been shown to involve multiple steps including polar release from anchoring protein PopZ, slow movement, and fast ParA-dependent movement to opposite cell pole. In this study, we demonstrate that ParA’s competing attractions from PopZ and from DNA are critical for segregation ofparS. Interfering with this balance of attractions – by expressing a variant ParA-R195E unable to bind DNA and thus favoring interactions exclusively between ParA-PopZ – results in cell death. Our data revealed that ParA-R195E’s sole interactions with PopZ obstruct PopZ’s ability to release the polar anchoring ofparSresulting in cells with multipleparSloci fixed at one cell pole. We show that the inability to separate and segregate multipleparSloci from the pole is specifically dependent on the interaction between ParA and PopZ. Interfering with interactions between PopZ and the partitioning protein ParB, which is the interaction that anchorsparSat the cell pole, does not rescue the ability of cells to separate the fixedparSloci when expressingparA-R195E. Thus, ParA and PopZ appear to have a distinct conversation from ParB yet can impact the release of ParB-parSfrom the anchoring at the cell pole. Collectively, our results reveal that the initial steps in chromosome segregation are highly regulated.
Similar enzymatic functions in distinct bioluminescence systems: Evolutionary recruitment of sulfotransferases in ostracod light organs
Authors: Lau Emily S , Goodheart Jessica A , Anderson Nolan T , Liu Vannie L , Mukherjee Arnab , Oakley Todd H 
Published: April 2023

AbstractGenes from ancient families are sometimes involved in the convergent evolutionary origins of similar traits, even across vast phylogenetic distances. Sulfotransferases are an ancient family of enzymes that transfer sulfate from a donor to a wide variety of substrates, including probable roles in some bioluminescence systems. Here we demonstrate multiple sulfotransferases, highly expressed in light organs of the bioluminescent ostracodVargula tsujii, transfer sulfate in vivo to the luciferin substrate, vargulin. We find luciferin sulfotransferases of ostracods are not orthologous to known luciferin sulfotransferases of fireflies or sea pansies; animals with distinct and convergently evolved bioluminescence systems compared to ostracods. Therefore, distantly related sulfotransferases were independently recruited at least three times, leading to parallel evolution of luciferin metabolism in three highly diverged organisms. Re-use of homologous genes is surprising in these bioluminescence systems because the other components, including luciferins and luciferases, are completely distinct. Whether convergently evolved traits incorporate ancient genes with similar functions or instead use distinct, often newer, genes may be constrained by how many genetic solutions exist for a particular function. When fewer solutions exist, as in genetic sulfation of small molecules, evolution may be more constrained to use the same genes time and again.
The bi-directional extracellular electron transfer process aids iron cycling byGeoalkalibacter halelectricusin a highly saline-alkaline condition
Authors: Yadav Sukrampal , Sadhotra Chetan , Patil Sunil A. 
Published: April 2023

AbstractBi-directional extracellular electron transfer (EET) is crucial to upholding microbial metabolism with insoluble electron acceptors or donors in anoxic environments. Investigating bi-directional EET-capable microorganisms is desired to understand the cell-cell and microbe-mineral interactions and their role in mineral cycling besides leveraging their energy generation and conversion, biosensing, and bio-battery applications. Here, we report on iron cycling by haloalkaliphilicGeoalkalibacter halelectricusvia bi-directional EET under haloalkaline conditions. It efficiently reduces Fe3+-oxide (Fe2O3) to Fe0at a 2.29±0.07 mM/day rate linked to acetate oxidation via outward EET and oxidizes Fe0to Fe3+with a 0.038±0.002 mM/day rate via inward EET to reduce fumarate. Bioelectrochemical cultivation confirmed its outward and inward EET capabilities. It produced 895±23 μA/cm2current by linking acetate oxidation to anode reduction via outward EET and reduced fumarate by drawing electrons from the cathode (−2.5±0.3 μA/cm2) via inward EET. The cyclic voltammograms ofG. halelectricus biofilms revealed redox moieties with different formal potentials, suggesting the involvement of different membrane components in bi-directional EET. The cyclic voltammetry and GC-MS analysis of the cell-free spent medium revealed the lack of soluble redox mediators, suggesting direct electron transfer byG. halelecctricus in achieving bi-directional EET. By reporting on the first haloalkaliphilic bacterium capable of oxidizing and reducing insoluble Fe0and Fe3+-oxide, respectively, this study advances the limited understanding of the metabolic capabilities of extremophiles to respire on insoluble electron acceptors or donors via bi-directional EET and invokes the possible role ofG. halelectricus in iron cycling in barely studied haloalkaline environments.Graphical abstract
SECRET AGENT O-GlcNAc Modifies GIGANTEA: Methods for Mapping of O-Linked β-N-Acetylglucosamine Modification Sites Using Mass Spectrometry
Authors: Kim Young-Cheon , Hartweck Lynn M. , Olszewski Neil E. 
Published: April 2023

AbstractArabidopsis thalianahas two glycosyl transferases, SPINDLY (SPY) and SECRET AGENT (SEC), that modify nuclear and cytosolic protein with O-linked fucose and N-acetylglucosamine (GlcNAc), respectively. SPY interacts physically and genetically with GIGANTEA (GI). Previously, we reported that SEC substrates are O-GlcNAc modified when they are co-expressed inE. coli. By analyzing overlapping sub-fragments of GI, we found a region that was modified by SEC. Mutational mapping of the modified region was then performed. Modification was undetectable when threonine 829 was mutated to alanine (T829A) while the T834A and T837A mutations reduced modification suggesting that T829 was the primary or only modification site. Mapping using several enrichment and mass spectrometry methods all detected only modification of T829.
Digital transformation strategies of project-based firms: case study of a large-scale construction company in China
Authors: Cao Dongping , Teng Xuejiao , Chen Yanyu , Tan Dan , Wang Guangbin 
Published: April 2023

Purpose
This study aims to explore how project-based firms, which generally organize most of their work around temporary projects in discontinuous and fragmented types of business contexts, proactively formulate and implement digital transformation strategies under institutional pressures in a predigital era.

Design/methodology/approach
An exploratory case study was conducted in a large-scale construction company in China using multiple data collection methods, including semistructured interviews, documentation collection and observation.

Findings
An integrated framework is developed to conceptualize three key dimensions of digital transformation strategies of project-based firms: strategic adaptation for organization-environment fit through balancing the internal efficiency needs with the external legitimacy pressures; proactive business transformation through comprehensively managing the roles of digital technologies in optimizing defined business processes and fostering new business models; and delicate organizational transformation to integrate temporary project-level operation processes with ongoing firm-level business processes.

Originality/value
This study represents an exploratory effort to empirically investigate how project-based firms strategically organize complex digital transformation imperatives in their discontinuous and fragmented business contexts. The findings contribute to deepened understandings of how complex organizational and environmental contexts can be comprehensively managed for systemic business and organizational transformations to leverage the value of emerging digital technologies for project-based organizations.
Does fintech lead to better accounting practices? Empirical evidence
Authors: Osei-Assibey Bonsu Mandella , Wang Ying , Guo Yongsheng 
Published: April 2023

Purpose
Innovation in fintech presents great opportunities and huge challenges for accounting practices around the world. This paper aims to examine the impact of Fintech on accounting practices including financial reporting, performance management, budgeting, auditing, risk and fraud management. Fintech is proxied by the adoption of AI and big data analysis in accounting practices.

Design/methodology/approach
We chose African countries as our focus countries and surveyed chartered and qualified accountants in both Ghana and Nigeria. With 201 questionnaires qualified for our final analyses, we adopted the structural equation modelling to analyse the impact of Fintech on accounting practices.

Findings
The empirical results show that the impact of AI and big data on accounting practices is positive and significant, indicating that fintech could potentially mitigate the agency problem in accounting practices and lead to better accounting practices. Interestingly, we find that, in general, the impact of AI is larger than that of big data.

Originality/value
Our results provide significant insights to regulators, policymakers and managers about the future development of adopting fintech in the regulation and governance framework at both macro and micro levels for accounting practice.
The determinants of internal audit quality
Authors: Samagaio António , Felício Teresa 
Published: April 2023

PurposeThis study aims to understand the behavior of internal auditors towards quality and analyze if some organizational and individual factors influence internal audit quality.Design/methodology/approachThe sample is constituted by Portuguese internal auditors, and the methodology includes the use of partial least squares – structuring equation model (PLS-SEM) to test the hypothesis under study.FindingsThe results show that there is a negative relationship between reduced audit quality practices (RAQP) and organizational commitment and independence. The results found that time pressure positively affect RAQP. There is no evidence that perceived organizational support (POS) and risk profile are determinants of RAQP.Originality/valueThis work contributes by extending the literature about the determinants of internal audit quality, but also to the practice by understanding the factors that influence the behavior of internal auditors and by making recommendations that allow an improvement of the quality of internal auditing.
The impact of justice on information sharing and innovation performance: a social exchange theory perspective
Authors: Huo Baofeng , Liu Xu , Li Siyu 
Published: April 2023

PurposeWith more demand-driven innovation activities, manufacturers must proactively engage in information sharing activities with their customers for better innovation performance. This study aims to inquire into the impacts of information sharing activities between manufacturers and customers (including information system usage and information content sharing) on manufacturers’ innovation performance and considers interfirm justice (including distributive justice, procedural justice and interactional justice) as information sharing antecedents.Design/methodology/approachThe social exchange theory is applied to develop the conceptual model. The authors examine the conceptual model with the structural equation modeling approach using data collected from 213 Chinese manufacturers.FindingsInteractional justice promotes information system usage. Both interactional justice and procedural justice increase information content sharing, while distributive justice decreases it. Information content sharing directly improves innovation performance and fully mediates the relationship between information system usage and innovation performance.Originality/valueThis research enriches empirical studies on justice-information sharing relationships by systematically investigating the impacts of three types of justice on different information sharing activities. It also adds to the application of social exchange theory in the practices of interfirm justice and information sharing. Besides, it probes into influencing mechanisms of different information sharing activities, information system usage and information content sharing, on innovation performance. The findings can guide firms to implement interfirm justice and information sharing practices for superior innovation performance.
Development and validation of a real-time testbed for renewable energy integration studies: South African grid code case study
Authors: Joubert Gideon Daniel , Raji Atanda Kamoru 
Published: April 2023

Purpose
Despite South Africa’s ailing electrical grid, substantial renewable energy (RE) integration is planned for the country. As grid-integrated RE affects all grids differently, this study aims to develop an adaptable grid code-guided renewable power plant (RPP) control real-time simulation testbed, tailored to South African grid code requirements to study grid-integrated RE’s behaviour concerning South Africa’s unique conditions.

Design/methodology/approach
The testbed is designed using MATLAB’s Simulink and live script environments, to create an adaptable model where grid, RPP and RPP guiding grid codes are tailorable. This model is integrated with OPAL-RT’s RT-LAB and brought to real-time simulation using OPAL-RT’s OP4510 simulator. Voltage, frequency and short-circuit event case studies are performed through which the testbed’s abilities and performance are assessed.

Findings
Case study results show the following. The testbed accurately represents grid code voltage and frequency requirements. RPP point of connection (POC) conditions are consistently recognized and tracked, according to which the testbed then operates simulated RPPs, validating its design. Short-circuit event simulations show the simulated wind farm supports POC conditions relative to short-circuit intensity by curtailing active power in favour of reactive power, in line with local grid code requirements.

Originality/value
To the best of the authors’ knowledge, this is the first design of an adaptable grid code-guided RPP control testbed, tailored to South African grid code requirements in line with which RPP behavioural and grid integration studies can be performed.
The shift in research trends related to artificial intelligence in library repositories during the coronavirus pandemic
Authors: Nugroho Prasetyo Adi , Anna Nove E. Variant , Ismail Noraini 
Published: April 2023

PurposeThis study sought to analyze the correlation between artificial intelligence (AI) and libraries and examine whether there were any shifts in research trends related to these two topics during the coronavirus pandemic.Design/methodology/approachThe study gathered secondary data from the Scopus website using the keywords “AI,” “library” and “repository,” from 1993 to 2022. Data were re-analyzed using the bibliometric software VOSviewer to examine the trending country's keyword relations and appearance and Biblioshiny to study the publication metadata.FindingsIndex keywords, such as “human,” “deep learning,” “machine learning,” “surveys” and “open-source software,” became popular during 2020, being closely related to digital libraries. Additionally, the annual scientific production of papers increased significantly in 2021. Words related to data mining also had the most significant growth from 2019 to 2022 because of the importance of data mining for library services during the pandemic.Practical implicationsThis study provides insight for librarians for the implementation of AI to support repositories during the pandemic. Librarians can learn how to maximize the AI-based repository services in academic libraries during the pandemic. Furthermore, academic libraries can create policies for repository services using AI.Social implicationsThis study can lead researchers, academicians and practitioners in conducting research on AI in library repositories.Originality/valueAs research on AI and digital repositories remains limited, the study identifies themes and highlights the knowledge gap existing in the field.
Collectively crafting the sales job: linking charismatic leadership to sales teams’ performance
Authors: Luu Tuan Trong 
Published: April 2023

PurposeIrrespective of the importance of collective job crafting for team performance, its antecedents have not been fully comprehended. Drawing upon social cognitive theory, this study proposes that sales managers’ charismatic leadership interacts with collective proactive personality in predicting collective job crafting, which in turn influences sales teams’ customer relationship performance and financial performance.Design/methodology/approachData were collected from 481 sales employees and 64 sales managers from 64 sales departments of tour companies. These multi-source data were analyzed through structural equation modeling.FindingsThe findings revealed that charismatic leadership was positively associated with sales teams’ collective job crafting, which was in turn positively related to sales teams’ customer relationship performance and financial performance. Collective proactive personality negatively moderated the impact of charismatic leadership on collective job crafting.Originality/valueThis study advances the extant knowledge by identifying the role of collective job crafting in translating charismatic leadership into sales teams’ performance.
Using ANTi-microhistory to theorize a typology of organizational crisis
Authors: Horner Carley , Holland Joseph , Novicevic Milorad 
Published: April 2023

PurposeThe purpose of this article is to present qualitative research of the past organizational crisis at Mississippi Chemical Corporation that the authors use to theorize a typology of organizational crisis from the leadership perspective.Design/methodology/approachThe authors combined ANTi-microhistory approach and staff ride research design to re-enact the sensemaking of the company’s former crisis leaders and elicit their retrospective oral accounts. A long interview format was used to interview the crisis leaders.FindingsThe authors found that the former crisis leaders not only conceptualized organizational crisis conventionally as an event or as a process but also that they engaged in the denial of the crisis to guard the company’s reputation and competed with their claims against the stakeholder claims about the ways how the crisis was to be managed.Originality/valueBased on the paper’s findings, the authors proposed an original typology of organizational crisis.