bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breast cancer patient-derived microtumors resemble tumor heterogeneity and enable protein-based stratification and functional validation of individualized drug treatment
Authors: Anderle Nicole , Schäfer-Ruoff Felix , Staebler Annette , Kersten Nicolas , Koch André , Önder Cansu , Keller Anna-Lena , Liebscher Simone , Hartkopf Andreas , Hahn Markus , Templin Markus , Brucker Sara Y. , Schenke-Layland Katja , Schmees Christian 
Published: August 2023

AbstractDespite tremendous progress in deciphering breast cancer at the genomic level, the pronounced intra- and intertumoral heterogeneity remains a major obstacle to the advancement of novel and more effective treatment approaches. Frequent treatment failure and the development of treatment resistance highlight the need for patient-derived tumor models that reflect the individual tumors of breast cancer patients and allow a comprehensive analyses and parallel functional validation of individualized and therapeutically targetable vulnerabilities in protein signal transduction pathways. Here, we introduce the generation and application of breast cancer patient-derived 3D microtumors (BC-PDMs). Residual fresh tumor tissue specimens were collected from n = 102 patients diagnosed with breast cancer and subjected to BC-PDM isolation. BC-PDMs retained histopathological characteristics, and extracellular matrix (ECM) components together with key protein signaling pathway signatures of the corresponding primary tumor tissue. Accordingly, BC-PDMs reflect the inter- and intratumoral heterogeneity of breast cancer and its key signal transduction properties. DigiWest®-based protein expression profiling of identified treatment responder and non-responder BC-PDMs enabled the identification of potential resistance and sensitivity markers of individual drug treatments, including markers previously associated with treatment response and yet undescribed proteins. The combination of individualized drug testing with comprehensive protein profiling analyses of BC-PDMs may provide a valuable complement for personalized treatment stratification and response prediction for breast cancer.
Association between acetaminophen metabolites and CYP2E1 DNA methylation level in neonate cord blood in the Boston Birth Cohort
Authors: Li Yijun , Hong Xiumei , Liang Liming , Wang Xiaobin , Ladd-Acosta Christine 
Published: August 2023

Abstract
Background
Acetaminophen is a commonly used medication by pregnant women and is known to cross the placenta. However, little is known about the biological mechanisms that regulate acetaminophen in the developing offspring. Cytochrome 2E1 (CYP2E1) is the primary enzyme responsible for the conversion of acetaminophen to its toxic metabolite. Ex vivo studies have shown that the CYP2E1 gene expression in human fetal liver and placenta is largely controlled by DNA methylation (DNAm) at CpG sites located in the gene body of CYP2E1 at the 5’ end. To date, no population studies have examined the association between acetaminophen metabolite and fetal DNAm of CYP2E1 at birth.

Methods
We utilized data from the Boston Birth Cohort (BBC) which represents an urban, low-income, racially and ethnically diverse population in Boston, Massachusetts. Acetaminophen metabolites were measured in the cord plasma of newborns enrolled in BBC between 2003 and 2013 using liquid chromatography-tandem mass spectrometry. DNAm at 28 CpG sites of CYP2E1 was measured by Illumina Infinium MethylationEPIC BeadChip. We used linear regression to identify differentially methylated CpG sites and the “DiffVar” method to identify differences in methylation variation associated with the detection of acetaminophen, adjusting for cell heterogeneity and batch effects. The false discovery rate (FDR) was calculated to account for multiple comparisons.

Results
Among the 570 newborns included in this study, 96 (17%) had detectable acetaminophen in cord plasma. We identified 7 differentially methylated CpGs (FDR < 0.05) associated with the detection of acetaminophen and additional 4 CpGs showing a difference in the variation of methylation (FDR < 0.05). These CpGs were all located in the gene body of CYP2E1 at the 5’ end and had a 3–6% lower average methylation level among participants with detectable acetaminophen compared to participants without. The CpG sites we identified overlap with previously identified DNase hypersensitivity and open chromatin regions in the ENCODE project, suggesting potential regulatory functions.

Conclusions
In a US birth cohort, we found detection of cord biomarkers of acetaminophen was associated with DNAm level of CYP2E1 in cord blood. Our findings suggest that DNA methylation of CYP2E1 may be an important regulator of acetaminophen levels in newborns.
A BPM-based approach for ensuring an agile and adaptive learning process
Authors: Bergaoui Nisseb , Ghannouchi Sonia Ayachi 
Published: August 2023

AbstractAgility is a contemporary approach to IT project management, which we can also use in education. Students learn through the gradual implementation of iterative projects with information exchange between team members. Agility is above all a mindset. Being agile is quite simply being able to adapt to an environment that changes. Furthermore, various research works focused on the assessment of innovative teaching methods to promote the acquisition of new professional skills (e.g. project-based learning, active and collaborative learning, smart learning, etc.). In addition, adaptive learning is a pedagogical method favoring tailor-made e-learning to respond to the acquisition of certain skills, through the adaptation of pedagogical resources according to the learners’ needs. Therefore, to establish a model based on these different methods to benefit from their advantages, we based our work on the Business Process Management approach, which constitutes the means of implementing thedesired agility in the learning process. Thanks to such a cyclical and continuous improvement approach, the learning process will evolve and take into account both the needs and the specificities of the involved actors (learners or teachers). We implemented our learning process and applied Process Mining techniques to foster the adoption of “Smart Education”. We also attempted to ensure learning process adaptability based on the scrutiny of the log files obtained throughprevious executions of our learning process.
Effects of High-Intensity Interval Training (HIIT) on Physical Performance in Female Team Sports: A Systematic Review
Authors: Stankovic Mima , Djordjevic Dusan , Trajkovic Nebojsa , Milanovic Zoran 
Published: August 2023

AbstractBackgroundThere is limited information regarding adaptation of HIIT in female athletes which is important since the adaptation to HIIT may be different compared to male athletes. Therefore, the aim of this systematic review was to summarize the effects of HIIT on physical performance in female team sports athletes.MethodsThe following databases Google Scholar, PubMed, Web of Science, Cochrane Library, ProQuest and Science Direct were searched prior to September 2nd, 2022. The inclusion criteria were longitudinal studies written in English, elite, sub-elite or college female team sports participants, and HIIT intensity had to be at 80–100% maximal heart rate. There were no exclusion criteria regarding the age of the participants or their training experience. The primary outcome measures were maximal oxygen uptake (VO2max), repeated sprint ability (RSA), change of direction speed, speed, explosive strength and body composition.ResultsA total of 13 studies met the inclusion criteria, with a total of 230 participants. HIIT improved VO2max in five studies (ES from 0.19 to 1.08), while three studies showed improvement in their RSA (ES from 0.32 to 0.64). In addition, change of direction speed was improved in five studies (ES from 0.34 to 0.88), while speed improved in four studies (ES from 0.12 to 0.88). Explosive strength results varied (ES from 0.39 to 1.05), while in terms of body composition, the results were inconsistent through observed team sports.ConclusionHIIT has significant effects on VO2max, RSA, change of direction speed, speed and explosive strength in female team sports, regardless of the competition level.
Large-scale solar system design, optimal sizing and techno-economic-environmental assessment
Authors: Thadani Hashwini Lalchand , Go Yun Ii 
Published: August 2023

AbstractMalaysia targets to achieve an energy mix that is inclusive of at least 20% of renewable energies by the year 2025. Large-scale solar photovoltaic system (LSS-PV) emerged as the most preferable choice in Malaysia. Energy Commission (EC) Malaysia has launched competitive bidding on LSS since 2016 with a capacity of 500 MW in Peninsular Malaysia and targets to add the solar capacity in Peninsula Malaysia to 500 MW by 2021. Solar energy is a very intermittent source which causes voltage variation. This project aims to overcome the shortcomings of the intermittency of solar energy by identifying an optimum PV panel sizing and configuration that reduces the intermittency of the supply. The project was carried out in three distinctive stages; first suitable sites were selected based on the EC proposed locations and further scrutinized to locations near the Main Transmission intake. The second stage was to size of PV panels and the inverters. The optimization was carried out at the PV module level. Suitable inverters for the said configuration was identified with a tool that evaluates the technical aspects of renewable energy power systems. The final stage was the economic analysis and environmental assessment. The optimization method has shown improvement in the system production from a range of 1.7–3.9% in the 30 MW plants. Countries with similar climates as Malaysia like Indonesia, Philippines and Thailand can adopt this optimizing method to size PV farms.
Interdependence of social-ecological-technological systems in Phoenix, Arizona: consequences of an extreme precipitation event
Authors: Helmrich Alysha , Kuhn Amanda , Roque Anaís , Santibanez Ameyalli , Kim Yeowon , Grimm Nancy B. , Chester Mikhail 
Published: August 2023

AbstractComplex adaptive systems – such as critical infrastructures (CI) – are defined by their vast, multi-level interactions and emergent behaviors, but this elaborate web of interactions often conceals relationships. For instance, CI is often reduced to technological components, ignoring that social and ecological components are also embedded, leading to unintentional consequences from disturbance events. Analysis of CI as social-ecological-technological systems (SETS) can support integrated decision-making and increase infrastructure’s capacity for resilience to climate change. We assess the impacts of an extreme precipitation event in Phoenix, AZ to identify pathways of disruption and feedback loops across SETS as presented in an illustrative causal loop diagram, developed through semi-structured interviews with researchers and practitioners and cross-validated with a literature review. The causal loop diagram consists of 19 components resulting in hundreds of feedback loops and cascading failures, with surface runoff, infiltration, and water bodies as well as power, water, and transportation infrastructures appearing to have critical roles in maintaining system services. We found that pathways of disruptions highlight potential weak spots within the system that could benefit from climate adaptation, and feedback loops may serve as potential tools to divert failure at the root cause. This method of convergence research shows potential as a useful tool to illustrate a broader perspective of urban systems and address the increasing complexity and uncertainty of the Anthropocene.
Agranulocytosis: a rare complication of the thionamides
Authors: El-Shareif Hawa Juma 
Published: August 2023

AbstractAgranulocytosis is a rare and life-threatening side effect of antithyroid drugs (ATD); it has been reported in < 0.2% of patients, usually within the first 3 months after starting ATD. We present a 62-year-old Libyan female patient who developed agranulocytosis 3 months after starting treatment with methimazole for hyperthyroidism. After 1 month of doubling her ATD dose, she developed a fever, sore throat, and generalized weakness for several days. Laboratory examinations revealed agranulocytosis (total leukocyte count was 1.14 × 103/µL, with granulocytes at 2.99% and lymphocytes at 93.21%). She was hospitalized, ATD was discontinued, and empirical antibiotic treatment was given. She was started on granulocyte colony-stimulating factor. Her cell counts improved, and she was discharged. The present case report aims to increase awareness of this potentially lethal adverse effect of ATD treatment. Patient education and close monitoring of high-risk patients are the key to reduce its morbidity and mortality.
Vascular graft infection with duodenal fistulization 10 years after hybrid endovascular aortic repair with renovisceral debranching: a case report
Authors: Osada Hiroaki , Yamazaki Kazuhiro , Takeda Takahide , Minatoya Kenji 
Published: August 2023

Abstract
Background
A secondary aortoenteric fistula after an aortic graft replacement or endovascular aortic repair is a rare but devastating complication that leads to extremely high morbidity and mortality. Because reports of vascular graft infection with enteric fistulization complicating in a hybrid aortic repair with renovisceral debranching are limited, the management method such as the extent of removal of the infected graft, reconstruction procedures, and the ideal type of graft are still debatable.

Case presentation
We report a successful case of a 73-year-old man presenting a vascular graft infection with duodenal fistulization, 10 years after hybrid endovascular aortic repair with renovisceral debranching for a 60-mm diameter of supra-renal abdominal aortic aneurysm. The patient had a history of polymyalgia rheumatica on oral prednisolone, perigraft seroma, deep vein thrombosis, and an allergy to rifampicin. The patient eventually recovered after partial removal of the grafts, in situ reconstruction using Fusion Bioline vascular prosthesis, primary duodenal repair, application of omental flap, and antibiotics without any evidence of re-infection after 1.5 years.

Conclusions
Although hybrid endovascular aortic repair is considered advantageous, especially for the elderly and high-risk patients, due to the avoidance of extracorporeal circulation and thoracotomy; once a devastating complication happens, an optimal treatment method should be considered for patients with several comorbidities. Although our procedures provided favorable results, careful monitoring to avoid re-infection is mandatory.
Seismic differential semblance-oriented migration velocity analysis — Status and the way forward
Authors: Chauris Hervé , Farshad Milad 
Published: August 2023

Differential semblance optimization (DSO) is a seismic waveform inversion approach. It has been developed to remove the limitation of conventional full-waveform inversion as a velocity model building tool when only reflected waves are available. It has connections with migration velocity analysis (MVA) techniques defined in the image domain to build macrovelocity models, with a split between the macromodel and the small-scale heterogeneities. Among the various DSO approaches, DSO-MVA has unique characteristics for velocity model building of complex structures, while ensuring the convergence to the global minimum in a local optimization approach: the wavefields are computed with the two-way wave equation operators, focusing panels are built in a survey-sinking mode, and the quality of the macrovelocity model is evaluated on common-image gathers in the subsurface-offset domain through a differential annihilator. We review the significant aspects that have been better understood over the past 15 years and discuss the way forward. The first identified issue has been the imprint of the small-scale heterogeneities on the macromodel update that is revealed with the use of wave equation-based Green’s functions and an ad hoc solution is subsequently developed by slightly modifying the definition of the objective function. A second important aspect has been the development of efficient approaches to compute the required quantitative migration based on approximate inverses to replace the previously used migrations. The issue of correctly interpreting the amplitudes has also moved into one of an improved model parameterization, i.e., from constant-density acoustics media to full acoustic media as a path toward fully viscoelastic media. Finally, we discuss the way forward with the challenges of extending DSO-MVA beyond the single scattering approximation, for processing diving waves and multiples, and also the challenge of numerical implementation, which remains critical even in two dimensions, for applicability to large-scale real data.
Observation of a Pressure Effect on an Al-substituted λ-Ti3O5 Heat-storage Material
Authors: Jia Fangda , Yoshikiyo Marie , Makuta Ryo , Kawakami Koutarou , Tokoro Hiroko , Ohkoshi Shin-ichi 
Published: August 2023

Abstract
We report a pressure effect on a heat-storage material, Al-substituted lambda-trititanium-pentoxide (λ-AlxTi3−xO5, 0 &lt; x ≤ 0.29), showing a phase transition to β-AlxTi3−xO5 by pressure application. The transition pressure is 23 MPa and 43 MPa for x = 0.01 and 0.04, respectively, which are available pressures for practical applications. First-principles electronic structure calculations of λ-Al0.25Ti2.75O5 were conducted to investigate the substitution site selectivity of the Al3+ ion and the substitution effect on the pressure-induced phase transition.
Drying out fish ponds, for an entire growth season, as an agroecological practice: maintaining primary producers for fish production and biodiversity conservation
Authors: GIRARD Léo , WEZEL Alexander , ROBIN Joël 
Published: August 2023

Agroecology largely focusses on terrestrial agroecosystems, but it can also be applied to fish farming. Indeed, ponds are typically used for fish production in Europe, but are also important reservoirs of biodiversity. Numerous studies demonstrate that both fish production and biodiversity are strongly determined by human management. One key practice in extensive fish farming, although more rare in Europe, is to dry out ponds. They are left dry for a complete year after several years of fish production. However, the extent to which this practice affects the functioning of the ecosystem, its biodiversity and fish production remain unclear. We investigated data from 85 fish ponds in the Dombes region, France, sampled between 2007 and 2014. We related variation in key abiotic characteristics to the time since last dry out. The dataset included organic matter content in pond sediments and concentrations of inorganic nitrogen and phosphorus in the water column, and biotic components such as macrophytes cover and richness, phytoplankton concentration and richness, macroinvertebrates density, and fish yield. Our results show that drying out facilitates the mineralization of organic matter in sediments and results in higher concentrations of inorganic nitrogen in the water column. Macrophytes cover is highest during the first year after drying out, and gradually declines after at the expense of increasing phytoplankton concentration. The diversity of both is highest in the first year after drying out and declines rapidly, especially for macrophytes. Fish yield is at its maximum in the second year. Drying out fish ponds appears to be an important agroecological practice in extensive fish farming with an application every three to four years. By nutrient recycling, this practice has a positive impact on the balance between primary producers and indirectly on the whole food web during two years. It optimizes fish production and allows biodiversity conservation.
Can multiple sclerosis nurses help reduce non-elective admissions? Revisiting the question 2 years on from an analysis of a service delivery audit
Authors: Metcalfe Gale , Owen Audrey 
Published: August 2023

Background: The results of a 2019/20 audit exploring the reasons for emergency hospital admissions among people with MS, identified urinary tract infections (UTIs) as a major reason. Consequently, the authors implemented several interventions with the aim of reducing non-elective admissions coded to UTI. This article disseminates whether the interventions implemented by the authors resulted in a reduction in non-elective UTI associated hospital admissions. Methods: The authors conducted a 12-month audit of non-elective admissions across two NHS Trusts, comparing and contrasting the data from the 2019/20 audit and national and local Hospital Episode Statistics. Findings: A reduction in non-elective admissions coded to UTI has significantly reduced across both sites, which is thought to be a consequence of the authors' proposed recommendations from the 2019/20 audit. Local data now identifies the main reasons for MS non-elective hospital admissions as respiratory issues and falls. Conclusions: Having raised awareness of the need to focus on improved bladder management, data now reveals further reduction in MS bladder and UTI related admissions. Further recommendations have been made, as there needs to be an increased focus on addressing improvements in both respiratory management and falls prevention.
Mechanical ventilation in acute myocardial infarction: Outcomes from a prospective audit at a cardiovascular hospital in Pakistan
Authors: Ansari Muhammad Imran , Umair Madiha , Taimoor Lalarukh , Memon Aziz-ur Rehman , Abubaker Zohaib , Arif Muhammad Sohaib , Qamar Nadeem , Karim Musa , Abubaker Jawed 
Published: August 2023

Background
This clinical audit aims to evaluate the clinical data regarding the management and outcomes of acute myocardial infarction (AMI) patients requiring mechanical ventilator (MV) support, along with identifying factors associated with prolonged MV support and 180-day mortality.

Materials and methods
In this study, we audited clinical data regarding management, in-hospital and short-term outcomes of adult patients with AMI required MV support. Patients with prolonged MV duration (>24h) and/or 180-day mortality were compared with their counterparts, and associated factors were identified. The binary logistic and Cox regression analyses were performed to determine the predictors of prolonged MV duration and 180-day mortality.

Results
In a sample of 312 patients, 72.8% were male, and the mean age was 60.3±11.5 years. The median MV duration was 24 [24–48] hours, with 48.7% prolonged MV duration. The admission albumin level was found to be the independent predictor of prolonged MV duration with an adjusted OR of 0.42 [0.22–0.82]. Overall 7.4% were re-intubated, 6.7% needed renal replacement therapy, 17.6% required intra-aortic balloon pump (IABP) placement, and 16.7% required temporary pacemaker placement. The survival rate was 80.4% at the time of hospital discharge, 74.7% at 30-day, 71.2% at 90-day, and 68.6% at 180-day follow-up. Age, prolonged MV duration, and ejection fraction were found to be the independent predictors of cumulative 180-day mortality with adjusted HR of 1.04 [1.02–1.07], 1.02 [1.01–1.03], and 0.95 [0.92–0.98], respectively.

Conclusions
Prolonged ventilator duration has significant prognostic implications; hence, tailored early recognition of high-risk patients needing more aggressive care can improve the outcomes.
Short complete diagnostic tests for circuits with two additional inputs in some basis
Authors: Popkov Kirill A. 
Published: August 2023

Abstract
We prove that any Boolean function in n variables can be modeled by a testable Boolean circuit with two additional inputs in the basis “conjunction, oblique conjunction, disjunction, negation” so that the circuit admits a complete diagnostic test of the length at most 2n + 3 with respect to stuck-at faults of the type 1 at gate outputs.