bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of the Maternal Gut Microbiota in Immune Activation at the Maternal-Fetal Interface Impact on Preeclampsia
Authors: Rescigno Maria , Giugliano Silvia , Carloni Sara , Schorn Tilo 
Published: May 2023

Abstract
Preeclampsia is a leading cause of morbidity and mortality in pregnant women, affecting 5-8% of gestations worldwide. Several factors such as abnormalities in maternal immunity, metabolic disorders and gut dysbiosis are known to contribute to disease development.
Here, we show that dysbiosis in pregnant C57BL/6J dams increased fetal resorption, impaired placental development and altered vascularization. These changes positively correlated with increased hypoxia and reduced uterine NK cell number. Moreover, intestinal dysbiosis significantly perturbated placental carbohydrate metabolism impacting NK cell IFN-γ secretion. Of note, glucose supplementation restored placental NK cell function in vitro, suggesting that the impairment was reversible and dependent on a lower glycolytic rate.
In this work, maternal gut microbiota emerges as a key player in carbohydrate metabolism during pregnancy with a pivotal role in controlling placental immunity and pregnancy outcome. These results provide insights for the identification of metabolic biomarkers and propose the microbiota as a potentially amenable target for preventive intervention of preeclampsia.
CircDock6 promotes NAFLD by regulating IGF1R through targeting mmu-let-7 g-5p
Authors: Li Peifei , Yu Siyi , Ding Xiaoyun , Xu Lei 
Published: May 2023

Abstract
Circular RNAs (circRNAs) are a novel kind of noncoding RNA with a closed ring structure. An increasing number of studies have shown that it plays an important role in NAFLD. In this study, we used circDock6 to explore its role and molecular mechanism in the progression of NAFLD. qRT‒PCR was used to detect the circDock6 expression level in SD liver tissues and HFD tissues. The regulatory effects of circDock6 in NAFLD were evaluated by Oil Red “O” staining, triglyceride detection, and western blot (WB) analysis. CircDock6 was significantly upregulated in HFD liver tissues compared to SD tissues. Additionally, Oil Red “O” staining and triglyceride detection revealed that decreased expression levels of circDock6 inhibited lipid droplet formation and reduced triglyceride content. CircDock6 acts as a mmu-let-7 g-5p sponge to promote the progression of NAFLD by regulating IGF1R. Our data demonstrated that circDock6 was expressed at high levels in HFD tissues. Knockdown of circDock6 expression inhibited NAFLD progression by regulating IGF1R through targeting mmu-let-7 g-5p.
Arteriovenous fistula : Rare but serious complication of the temporomandibular joint arthroscopy
Authors: Santo Luciano Del , Nasim Omar , Leal João Victor , Ramos Thiago , Cavalcante Mateus , Villari Thiago , Sincos Igor 
Published: May 2023

Abstract
Background: Temporomandibular joint (TMJ) arthroscopy is a safe and minimally invasive procedure involving arthroscopic examination and operative surgery for joint arthrogenous disorders. Common complications of surgery include fluid extravasation, bleeding, and damage to the joint and associated structures. However, most of them are transient and easily controlled. Of the very rare complications is an arteriovenous fistula (AVF), which is an abnormal connection between an artery and a vein as a result of iatrogenic injury from TMJ arthroscopy.
Case presentation: We present a case of 52-year-old woman who presented with a whooshing sound, an audible bruit and a palpable thrill related left TMJ region after TMJ arthroscopy. The patient was suspected of vascular lesion and was diagnosed with an AVF involving a left transverse facial artery and superficial temporal vein by selective angiography. The patient underwent successful embolisation of the AVF under local anesthesia with intra-operative confirmation using angiography and clinical resolution of the symptoms.
Conclusion: Due to its rarity, clinicians need to be aware of its presentation and the need for a multidisciplinary approach in order to provide timely and efficient treatment. To the best of our knowledge, we report the first case of AVM secondary to TMJ arthroscopy involving transverse facial artery and superficial temporal vein.
Characterizations of a clinical Gallibacterium anatis strain from poultry in the respiratory tract：a case report
Authors: wu shihai , wei miaoyi , liu haoyang , yu chenyun , gong shimiao , si xiaohui , he zhiyuan , liu xiaoye , dong hong 
Published: May 2023

Abstract
Background Gallibacterium anatis, is a gram-negative coccoba-cillus that belong to the family of Pasteurellaceae. It is the historically considered an opportunistic pathogen of intensively reared poultry and domestic birds. It is the main cause of salpingitis, ovaritis and peritonitis. G. anatis infection lead egg yield and egg quality of laying hens will be reduced after infection. G. anatis has seriously affected the overall productivity of the world poultry industry and caused economic losses in the poultry industry.Case presentation Herein, we reported that this pathogen can cause obvious respiratory diseases in hens and serious harm to the reproductive tract of laying hens. The clinical symptoms of sick chickens include tracheal hemorrhage, yellow inflammatory secretion, pulmonary hemorrhage, tubal edema, yolk rupture and yolk peritonitis. We isolated and cultured the pathogenic bacteria. The characteristics of pathogenic bacteria were investigated by hemolytic activity assay, biochemical assay, and drug sensitivity test.Conclusions Based on the isolation, identification and characteristic detections of this isolations showed that the pathogen was gram-negative bacterium Gallibacterium anatis, which could ferment glucose, mannitol, inositol, and sorbitol. Catalase and oxidase test were positive. Cephalosporins and tetracyclines have good inhibitory effect on the pathogen, β- Lactam antibiotics will have strong resistance.
Robotic versus laparoscopic versus open ileoanal pouches in a busy District General Hospital setting
Authors: Patel Maitreyi S. , Aguayo Xavier , Kaul Sandeep , Banerjee Saswata , Boulton Richard , Huang Joseph , Hanson Matthew , Rajendran Nirooshun 
Published: May 2023

Abstract
Purpose
Ileo-anal pouch anastomosis (IPAA) is an optimal procedure for patients with chronic ulcerative colitis and familial adenomatous polyposis (FAP), for whom restoration of continuity is a priority. Due concerns of long term outcomes, there have been discussions about centralisation of this procedure. We report the evolution of the service at our trust. The aim of this study was to present our series of consecutive patients who underwent IPAA (open, laparoscopic and robotic) with respect to the surgical and functional outcomes.
Methods
Data were prospectively collected from consecutive IPAA cases from January, 2014 to March, 2023. Patient demographics, operative details, postoperative complications, functional outcomes were collected. Patients were selected by IBD specialized surgeons and reviewed by a pouch nurse. Follow-up consisted of clinical examination and assessment of pouch function.
Results
Thirty-six patients underwent IPAA over the study period. 18 underwent laparoscopic surgery, of these, two were converted to open. 6 underwent robotic, one was converted to open, making 15 open cases. The median operative time was 395minutes. Major complications (Clavien-Dindo III-IV) were seen in three patients (8.34%). Median post operative length of stay was 8 days. The 30-day re-admission rate was 30.56%, the most common cause being high stoma output. There were no mortalities.
The median Oresland Score was 6, and SIBDQ score was 51.
Conclusion
Our data suggests that with good patient selection and guidance from an experienced team, is safe and feasible with good peri-operative outcomes and acceptable pouch function in a District General Hospital setting.
Landscape use and habitat configuration effects on amphibian diversity in southern Brazil wetlands
Authors: Oro Natália , Moser Camila Fernanda , Dalzochio Marina Schmidt , de Oliveira Marcelo Zagonel , Hadi Arel , Preuss Jackson Fábio , Tozetti Alexandro Marques 
Published: May 2023

Abstract
In this study, we evaluated the effect of changes in natural wetlands on the amphibian diversity in spatial and temporal scales. We sampled 10 wetland sites along floodplains in southern Brazil. We classify the sites as reference or altered ponds according to the preservation degree and presence of human impact. The amphibian monitoring was conducted by calling surveys performed between 2015/2016 using an automated recording system that identified the calling male species. We identified 23 species, mainly distributed in the families Hylidae (43%) and Leptodactylidae (34.8%). The altered ponds had lower diversity and higher species dominance. Even ponds with the greatest landscape change revealed an expressive resilience concerning the amphibian species composition. However, only Boana pulchella was dominant in altered ponds and B. pulchella and Pseudopaludicola falcipes were dominant in reference ponds. The landscape changes favored the reduction of amphibian richness which was driven by the expansion of the urban area and loss of flooding areas. From 1999 to 2016 all sampled sites had their wetlands reduced is area as the surrounding urban area increased, which is an unfavorable scenario for the maintenance of biodiversity. This diagnosis reveals the combined loss of habitat and reproductive sites of anurans in subtropical wetlands.
SLC35G2 as a Prognostic Biomarker in Hepatocellular carcinoma and Its Correlation with Immunity
Authors: Meng Yanqiu , Yuan Lebing , Huang Xianbin , Li Youhua , Fu Sansan , Peng Xiaodong 
Published: May 2023

Abstract
Hepatocellular carcinoma (HCC) is the major cause of the worldwide cancer burden, especially in China. Solute Carrier Family 35 Member G2 (SLC35G2), a methylation-related gene, plays an essential role during tumorigenesis. However, its roles in key biological functions, the tumor microenvironment, mutations, and single-cell sequencing analysis remain unclear in HCC. This study aimed to identify the correlation between SLC35G2 and prognosis, biological roles, and immune features in HCC. The abnormal expression of SLC35G2 was associated with multiple tumor types, and there was a significant upregulation in HCC samples compared to normal tissues, which was an independent prognostic factor for predicting poor overall survival (OS) and disease-specific survival (DSS) in HCC. A nomogram based on SLC35G2, age, gender, histologic grade, and T-, N-, and M-stages was constructed, and the prognostic model performed well as shown by calibration curves for the 1-, 3-, and 5-year OS. Gene set enrichment analysis showed that SLC35G2 was closely related to tumorigenesis and immune response pathways, including Hippo-merlin, PI3K-AKT, IL-8, and IL-10 signaling pathways. In addition, SLC35G2 expression was inversely correlated with eosinophils and Th17 cells, and increased SLC35G2 expression was significantly associated with immune checkpoint molecules (GI24, CTLA4, PD-L1, B7-H3, TIM-3, and TGF-β). Furthermore, single-cell sequencing analysis showed that SLC35G2 expression was primarily localized in NK/T cells. In conclusion, SLC35G2 was identified as a new prognostic marker and had important potential implications for immunotherapy in HCC.
Epidemiology of Thoracolumbar Fractures: A Cross-Sectional Study
Authors: Junior Deoclides Lima B , Sá Brenno Franklyin A , POKORNY GABRIEL , Almeida Nivaldo S. , Silva Douglas Richard C da , Silva João Pedro , Nascimento José William Araújo do , Carneiro Geraldo de S. 
Published: May 2023

Abstract
Purpose: This study aims to analyze the epidemiological profile of operated patients victims of thoracolumbar fractures.
Methods: This was a retrospective cross-sectional descriptive study. The sample was composed of a total of 223 cases of thoracolumbar fractures between 2015 and 2019. Data (sex, age, site of origin, etiology of injury, Frankel scale of neurological deficit, and affected vertebral segment) were extracted from records of patients who were victims of trauma to the thoracic and lumbar spine and surgically treated in the institution.
Results: 223 patients were analyzed, of which 164 (73.5%) were male. The mean age was 35.9 ± 14.3 years. Most traumas were caused by motorcycle accidents (36.7%), followed by falls from heights (31.8%) and car accidents (17.0%). The main cause of trauma in men was motorcycle accidents (39.0%), while in women it fell from height (35.5%). The segment most affected was the thoracolumbar spine in 118 (52.9%) of the cases, followed by the thoracic segment in 78 (34.9%). 46.1% of patients admitted with Frankel E. Traffic accidents were the main causes of complete motor deficit at hospital admission (FRANKEL A).
Conclusions: Among surgically treated patients with trauma to the thoracic and/or lumbar spine, there was a predominance of motorcycle accidents followed by car accidents and men who were significantly prone to fractures. Furthermore, the thoracolumbar transition was the region most affected region; however, the injuries in the thoracic region were the ones that most exhibited the worst Frankel status.
Diabetes Mellitus Type 2 as a Risk Factor and Outcome Modifier for Cryptococcosis in HIV negative, non-transplant Patients, a Propensity Score Match Analysis
Authors: Kung Vanessa M. , Ferraz Carolina , Kennis Matthew , Franco-Paredes Carlos , Barahona Lilian Vargas , Shapiro Leland , III George R Thompson , Chastain Daniel B. , Henao-Martínez Andrés 
Published: May 2023

Abstract
Cryptococcosis is an opportunistic fungal infection of worldwide distribution with significant associated morbidity and mortality. HIV, organ transplantation, malignancy, cirrhosis, sarcoidosis, and immunosuppressive medications, are established risk factors for cryptococcosis. Type 2 diabetes mellitus (DM2) has been hypothesized as a risk factor and an outcome modifier for cryptococcosis. We aimed to compare outcomes among HIV-negative, non-transplant (NHNT) patients with and without DM2. We also characterized a cohort of patients with cryptococcosis and DM2 as the only identifiable risk factor. We queried a global research network to identify NHNT patients (n = 3,280). We performed a propensity score-matched (PSM) analysis comparing clinical outcomes among cryptococcosis patients with DM2 (n = 995) versus those without DM2 (n = 2,285). We also characterize adults with cryptococcosis and DM2 as the only risk factor. After PSM, NHNT patients with DM2 were more likely to develop cognitive dysfunction [9% vs. 6%, OR = 1.6; 95% CI (1.1–2.3); p = 0.01] but had similar mortality, hospitalization, ICU, and stroke risk after acquiring cryptococcosis when compared to NHNT patients without DM2. We identified 44 patients with cryptococcosis who had DM2 as the only identifiable risk factor for infection. Patients with cryptococcosis and isolated DM2 commonly had chronic kidney and end-stage renal disease. The most common anatomic site of cryptococcal infection was pulmonary. The annual incidence of cryptococcosis in patients with DM2 as their only identifiable risk factor was only 0.001%, and the prevalence was 0.002%. DM2 is associated with increased cognitive dysfunction risk in NHNT patients with cryptococcosis. It is rare for DM2 to be the only identified risk factor for developing cryptococcosis. Kidney disease, hyperglycemia, and immune dysfunction can increase risk in this setting.
Validity of BiliDx as a point-of-care bilirubin measurement device to diagnose neonatal jaundice and monitoring phototherapy at Muhimbili National Hospital, an observational study
Authors: Clemence Pascal , Moshiro Robert , Manji Karim 
Published: May 2023

Abstract
Background Neonatal jaundice is a condition caused by elevated levels of bilirubin in the bloodstream. Laboratory determination of serum bilirubin concentration by total serum bilirubin (TSB) test is still considered as gold standard for clinical guidance and practice. In developed countries diagnosis of neonatal jaundice is shifting towards point-of-care medical devices. So, this study aimed to determine the accuracy of the BiliDx device relative to a laboratory total serum bilirubin to diagnose and monitor jaundice among neonates admitted at Muhimbili National Hospital (MNH).Material and Methodology: This was a prospective hospital-based observational study conducted at the Neonatal Ward – MNH, Dar-es-Salaam, Tanzania from November 2022 to January 2023. A total of 181 neonates admitted at the neonatal ward with Jaundice and whose parents consented were enrolled in the study. Blood samples were collected; 2 mls of venous blood into the vacutainer bottle for routine laboratory measurement of total serum bilirubin (TSB) and 25µL blood collected into a transfer pipette tube and applied to BiliDx. STATA version 15.1 was used for data analysis.Results Out of 181 neonates, 39.2% (71/181) had birth weight between 1500g − 2499.9g, approximately 2/3rd (121/181) were preterm, 92/181 (50.8%) were males and 100/181 (55.2%) were undergoing phototherapy treatment the moment sample taken. The median bilirubin concentration was 76.9 [IQR: 54.7-129.9] mmol/l for BiliDx and 122.4 [IQR: 88.7–182.0] mmol/l for TSB. The minimum and maximum values obtained with BiliDx were, 3.4 and 427.5 mmol/l respectively, compared with 10.7 and 533.1 mmol/l using TSB. A linear relationship and correlation coefficient of 0.8408 (p = 0.000) between BiliDx and TSB was found. The regression analysis showed the presence of constant error [coefficient of BiliDx/slope = 0.91, 95% CI (0.82–0.99), p = 0.000] and random error exclusively [coefficient of constant/y-intercept = 48.52, 95%CI (37.70-59.34), p = 0.000]. The Bland–Altman plot showed an acceptable mean difference of 39.1mmol/l, limits of agreement of -48.3mmol/l to 126.4mmol/l and 179 points (179/181 = 98.9%) lying inside the limits of agreement.Conclusion The results support the use of BiliDx for rapid and accurate testing of elevated levels of bilirubin in the bloodstream among neonates since 98.9% of the differences between BiliDx and standard laboratory TSB lie between the lines of agreements.
Climate change impacts on ocean light in Arctic ecosystems beyond sea ice
Authors: Kristiansen Trond , Varpe Øystein , Selig Elizabeth , Laurel Benjamin , Sydeman William , Hegglin Michaela , Wallhead Phil 
Published: May 2023

Abstract
Climate change will drive sea ice loss, resulting in increased light availability across polar marine ecosystems, but the consequences of these changes are largely unknown. We quantify how future conditions for sea ice and snow, storm-driven waves, clouds, ozone, and chlorophyll will affect the seasonal absorption and reflection of light in Arctic seas. Using CMIP6 inputs and a spectral radiative transfer model, we predict a 20-50% increase in visible light by 2100 in the Northern Bering/Chukchi and Barents Seas ecosystems. For cold-water fish species, warmer summers and reduced phytoplankton will decrease survival in summer, while asynchrony in prey and light availability will decrease survival in fall. Warmer-water species will be less impacted. Coupled with ocean warming, increased light availability will accelerate changes in Arctic ecosystems, compromising the growth and survival of Arctic species in transitional zones and facilitating the northward expansion of boreal species.
Microbial iron reduction under oxic conditions: when microfluidics meets geochemistry
Authors: Ceriotti Giulia , Berg Jasmine , Borisov Sergey , Santos Alice Bosco 
Published: May 2023

AbstractIron (Fe) reduction is one of Earth's most ancient microbial metabolisms, but after atmosphere-ocean oxygenation, this anaerobic process was relegated to niche anoxic environments below the water and soil surface. However, new technologies to monitor redox processes at micrometer scales relevant to microbial cells have the potential to reveal how oxygen (O2) concentrations control the distribution of aerobic and anaerobic metabolisms. To explore the impact of varying O2levels on microbial Fe reduction, we cultivated a facultative Fe-reducing bacterium in a novel microfluidic reactor integrated with transparent planar O2sensors. Contrary to expectations, microbial growth induced Fe(III)-oxide (ferrihydrite) reduction under fully oxygenated conditions, without the formation of O2-depleted micro-sites. Our observations fundamentally change our understanding of Fe cycling in ancient and contemporary environments, from our interpretation of Fe mineralogy in the rock record to heavy metal and nutrient mobility in the modern subsurface.
A novel approach to predicting early pregnancy outcomes dynamically in a prospective cohort using repeated ultrasound and serum biomarkers
Authors: Petersen Jesper Friis , Friis-Hansen Lennart Jan , Bryndorf Thue , Jensen Andreas Kryger , Andersen Anders Nyboe , Løkkegaard Ellen Christine Leth 
Published: May 2023

Abstract

This study aimed to develop a dynamic model for predicting outcome during the first trimester of pregnancy using baseline demographic data and serially collected blood samples and transvaginal sonographies. A prospective cohort of 203 unselected women with an assumed healthy pregnancy of <8 weeks’ gestation was followed fortnightly from 4–14 weeks’ gestation until either miscarriage or confirmed first trimester viability. The main outcome was development of a model to predict viability from gestational age-dependent fetal viability hazard ratios using both baseline and updated serial data from each visit. Secondary outcomes were risk factors for miscarriage. The results showed that 18% of the women experienced miscarriages. A fetal heart rate detected before 8 weeks’ gestation indicated a 90% (95%CI 85–95%) chance of subsequent delivery. Maternal age (≥35 years), insufficient crown-rump-length (CRL) and mean gestational sac diameter (MSD) development, and presence of bleeding increased the risk of miscarriage. Serum biomarkers, including hCG, progesterone, and estradiol, were found to impact the risk of miscarriage with estradiol as the most important. The best model to predict miscarriage was a combination of maternal age, vaginal bleeding, CRL, and hCG. The second-best model was the sonography-absent model of maternal age, bleeding, hCG, and estradiol. This study suggests that combining maternal age, and evolving data from hCG, estradiol, CRL, and bleeding could be used to predict fetal viability during the first trimester of pregnancy.
Trial registration:
ClinicalTrials.gov identifier: NCT02761772