bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotyping and metabolome analysis reveal the role of AdoMetDC and Di19 genes in determining acquired tolerance to drought in rice
Authors: Sankarapillai Lekshmy V. , Vijayaraghavareddy Preethi , Nanaiah Karthik , Arpitha Gajamaranahally D. , Chaitanya Purushothama M. , Sathishraj Rajendran , Shindhe Dhananjay , Vemanna Ramu S. , Yin Xinyou , Struik Paul C. , Sreeman Sheshshayee 
Published: August 2023

AbstractWater‐saving attempts for rice cultivation often reduce yields. Maintaining productivity under drought is possible when rice genotypes are bred with improved metabolism and spikelet fertility. Although attempts have been made to introgress water mining and water use efficiency traits, combining acquired tolerance traits (ATTs), that is, specific traits induced or upregulated to better tolerate severe stress, appears equally important. In our study, we screened 90 rice germplasm accessions that represented the molecular and phenotypic variations of 851 lines of the 3 K rice panel. Utilising phenomics, we identified markers linked to ATTs through association analysis of over 0.2 million SNPs derived from whole‐genome sequences. Propensity to respond to ‘induction’ stress varied significantly among genotypes, reflecting differences in cellular protection against oxidative stress. Among the ATTs, the hydroxyl radical and proline contents exhibited the highest variability. Furthermore, these significant variations in ATTs were strongly correlated with spikelet fertility. The 43 significant markers associated with ATTs were further validated using a different subset of contrasting genotypes. Gene expression studies and metabolomic profiling of two well‐known contrasting genotypes, APO (tolerant) and IR64 (sensitive), identified two ATT genes: AdoMetDC and Di19. Our study highlights the relevance of polyamine biosynthesis in modulating ATTs in rice. Genotypes with superior ATTs and the associated markers can be effectively employed in breeding rice varieties with sustained spikelet fertility and grain yield under drought.
Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis
Authors: Barco‐Antoñanzas Maria , Font‐Farre Maria , Eceiza Mikel V. , Gil‐Monreal Miriam , van der Hoorn Renier A. L. , Royuela Mercedes , Zabalza Ana 
Published: August 2023

AbstractThe herbicides glyphosate and pyrithiobac inhibit the enzyme 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) in the aromatic amino acid biosynthetic pathway and acetolactate synthase (ALS) in the branched‐chain amino acid biosynthetic pathway, respectively. Here we characterise the protease activity profiles of a sensitive (S), a glyphosate‐resistant (GR) and a multiple‐resistant (MR) population of Amaranthus palmeri in response to glyphosate and pyrithiobac. Amino acid accumulation and cysteine protease activities were induced with both herbicides in the S population and with pyrithiobac in the GR population, suggesting that the increase in cysteine proteases is responsible for the increased degradation of the available proteins and the observed increase in free amino acids. Herbicides did not induce any changes in the proteolytic activities in the populations with target‐site resistance, indicating that this effect was only induced in sensitive plants.
Presidential particularism, the Paycheck Protection Program (PPP), and the Biden administration
Authors: Ha Heonuk 
Published: August 2023

AbstractFederal spending has been a strong governing instrument for the president and governing party, who have pursued their political and electoral benefits by strategically allocating federal grants, procurements, and loans to subnational governments. The Paycheck Protection Program (PPP), in which the federal government issued almost $1 trillion in loans over three rounds between April 2020 and May 2021 to support small businesses' operations in response to the COVID‐19 crisis, was no different. I build on previous research in distributive politics by examining the third round of PPP loans, in which $277 billion was allocated in the first months of the Biden administration. I find significant evidence of presidential particularism, as the Biden administration issued a large amount of PPP lending to Pro‐Biden and core Democratic counties as well as to congressional districts with copartisan (Democratic) house members. Furthermore, the magnitude of these presidential particularism findings immediately following the presidential election year is considerably larger compared to previous studies.
Impact of the corpus luteum and follicular parameters on the levels of plasma progesterone and the prediction of pregnancy in Holstein dairy cows
Authors: Sharawy Heba A. , Hegab AbdelRaouf O. , Elmetwally Mohammed A. 
Published: August 2023

AbstractThe objective of this study was to investigate the reliability of corpus luteum and dominant follicles, imaging parameters on the plasma progesterone, and prediction of pregnancy in cows. In total, 48 cows were used in this study and underwent Ovsynch program. Ultrasound imaging of the ovaries was done at the time of PG shots. Corpus luteum diameter (CL_d), area (CL_area), volume (CL_vol), and pixels (CL_PXL), as well as dominant follicle diameter (F_d) and area (F_area), were estimated using the ImageJ program. Blood samples were taken to assess progesterone (P4) concentrations. Pregnancy status was determined at 32 ± 3 days after insemination using an ultrasound “Sonoscape‐5 V.” Data were analysed using correlation analysis and ROC curves. Plasma P4 concentration showed positive correlation with CL_d (r = .68, p < .01), CL_area (r = .45, p < .01), CL_volume (r = .41, p < .01), and CL_pixels (r = .67, p < .01). The ROC curve indicated that P4 concentrations and CL parameters, especially the CL_pixels, were the best predictors of the pregnancy, among the others that were able to detect pregnancy at the time of PG with the best P4 and CL_pixels cut‐off value (4.1 ng/mL and 43.18) and AUC was (0.95 and 0.89); resp. (p < .001). Regarding the other parameters, it was possible to set AUC 0.79, 0.79, and 0.68 for CL_d, area, and volume with a sensitivity of 66.7%, 70.8%, and 66.7% and a specificity of 88.2%, 82.4%, and 70.4% (p < .01), respectively. The AUC for both the follicular diameter and F_area was (0.56) with a sensitivity of 58.33% and 70.8% and a specificity of 62.5% and 50% (p > .05), respectively. In conclusion, CL measurements and progesterone concentrations had the greatest pregnancy prediction in Holstein dairy cows.
Mammary gland inflammation in rabbits does (Oryctolagus cuniculus): A systematic review
Authors: Vasiu Iosif , Wochnik Marco , Dąbrowski Roman 
Published: August 2023

AbstractMastitis is a common condition in rabbit (Oryctolagus cuniculus) does, especially in rabbitries where mammae inflammations are the leading pathological expression of Staphylococcus aureus infections in reproductive females. It affects both the litter and the lactating female, including subsequent lactations. Non‐septic (i.e. galactostasis and cystic mastitis) and septic (i.e. Mastitis acuta; including Mastitis gangrenosa; and Mastitis chronica) mammary inflammations are acknowledged; however, the literature is scarce regarding non‐septic inflammations or subclinical mastitis. Thus, the present systematic review highlights and summarizes the existing data concerning mastitis in rabbit does, including diagnosis and treatment plans.
Risk factors associated with adolescent suicidality before and during the COVID‐19 pandemic
Authors: Zhu Allison Y. , Crawford Michael H. 
Published: August 2023

AbstractObjectiveTo evaluate the impact of the coronavirus disease 2019 pandemic on youth suicidal behaviors.MethodThis study examined two national surveys of high school students, the 2019 Youth Risk Behavior Survey (YRBS) and the 2021 Adolescent Behaviors and Experiences Survey (ABES).ResultsThe YRBS 2019 had 13,677 entries: 18.6% (17.5–19.8) (weighted percentage and 95% confidence intervals [CIs]) of youth had suicidal ideation (SI) and 8.9% (7.9–10.0) had at least one suicide attempt (SA). The ABES 2021 had 7705 entries: 19.9% (18.0–22.0) of youth had SI and 9.0% (7.7–10.5) had SA. In ABES 2021, both the percentage of youth with SI or SA was highest at age 14, at 21.8% (16.9–27.8) and 10.0% (6.6–14.8), respectively. The top factors associated with both SI and SA were parental abuse, sexual violence, illicit drug use, misuse of prescription pain medicine, and being bullied electronically. Screen time ≥3 h per day (not including schoolwork) was associated with a lower risk of SA (odds ratio [OR] 0.553, 95% CI: 0.382–0.799), but not SI (OR 1.011, 0.760–1.344).ConclusionsEarlier onset of adolescent suicidality, at age 14, was noted during the pandemic. The association of higher non‐school work‐related screen time with lower SA is unexpected and warrants validation.
Two Paleoproterozoic tectono‐thermal events in the Central Orogenic belt, North China craton, determined from EPMA Th–U–Pb monazite geochronology of the Zanhuang massif
Authors: Wang Junpeng , Jiao Yunzhe , Jiang Kang , Kusky Timothy , Wang Lu , Deng Hao 
Published: August 2023

AbstractTectono‐thermal records older than ca. 1.8 Ga in the North China craton have been poorly resolved given that they have been strongly overprinted by younger events, despite being key to understanding the tectonic evolution of the craton. We report results of monazite Th–U–Pb isochron geochronology via electron probe microanalysis on the Zanhuang massif in the North China craton, to determine its detailed Precambrian tectono‐thermal history. Results suggest that the ca. 2.1 Ga tectono‐thermal event can be divided into distinct ca. 2.17 Ga and ca. 2.10 Ga stages, reflecting metamorphism related to retroarc regional extension of the North China craton. Similarly, the previously established ca. 1.8 Ga metamorphic event can be divided into distinct ca. 1.88 Ga and ca. 1.79 Ma stages. Through a detailed summary and comparison, we interpret that the ca. 1.88 Ga monazite age reflects the timing of peak metamorphism, which was followed by ca. 1.79 Ga retrogression.
Social media insights on public perception and sentiment during and after disasters: The European floods in 2021 as a case study
Authors: Li Weilian , Haunert Jan‐Henrik , Knechtel Julius , Zhu Jun , Zhu Qing , Dehbi Youness 
Published: August 2023

AbstractDetecting and collecting public opinion via social media can provide near real‐time information to decision‐makers, which plays a vital role in urban disaster management and sustainable development. However, there has been little work focusing on identifying the perception and the sentiment polarity expressed by users during and after disasters, particularly regional flood events. In this article, we comprehensively analyze tweets data related to the “European floods in 2021” over time, topic, and sentiment, forming a complete workflow from data processing, topic modeling, sentiment analysis, and topic and sentiment prediction. The aim is to address the following research questions: (1) What are the public perception and main concerns during and after floods? (2) How does the public sentiment change during and after floods? Results indicate that there is a significant correlation between a flood's trend and the heat of corresponding tweets. The three topics that receive the most public concern are: (1) climate change and global warming; (2) praying for the victims: and (3) disaster situations and information. Negative sentiments are predominant during the floods and will continue for some time. We tested five different classifiers, of which TextCNN‐attention turned out to deliver the best predictions in topic and sentiment prediction, and performed well for sparse flood tweets, it can be used to predict the topic and sentiment polarity of a single tweet in real‐time during the flood events. Our findings can help disaster agencies to better understand the dynamics of social networks and develop stronger situational awareness towards a disaster, which can contribute to scientifically justified decision‐making in urban risk management and also meet the challenges associated with the global sustainable development goal 11 (SDGs) on Sustainable Cities and Communities.
Clinical findings, surgical techniques, prognostic factors for short‐term survival and long‐term outcome in horses with acquired inguinal hernias: Ninety‐eight cases (2005–2020)
Authors: François Isé , Lepage Olivier M. , Schramme Michael C. , Salciccia Alexandra , Detilleux Johann , Grulke Sigrid 
Published: August 2023

AbstractObjectiveTo report findings, outcome and determine variables associated with survival in horses with acquired inguinal hernia (AIH).Study designRetrospective study.AnimalsA total of 98 cases in 97 horses.MethodsThe medical records (2005–2020) of horses diagnosed with AIH were reviewed. Retrieved data included signalment, history, clinical variables, surgical aspects, postoperative complications, and short‐ and long‐term outcomes. Logistic regression analyses were used to determine factors associated with short‐term survival (p < .05).ResultsManual reduction was attempted in a third of the cases (32/98, 33%) and emergency surgery to reduce the hernia was performed in 64 of 98 (65%) cases. Concurrent small intestinal (SI) volvulus was identified in 26 (26/98, 27%) cases. Castration was the most common technique used to prevent recurrence (64/94, 68%). Overall AIH recurrence rate was 11% (11/98). A total of 59 (59/98, 60%) cases survived to hospital discharge and 49 of 52 (94%) cases were still alive after 12 months. Cases admitted within 10 h of colic signs had increased odds of survival (72%) compared to those admitted after 10 h (26%; p < .001). Draft breeds (p = .021), high heart rate on admission (p = .001) and concurrent SI volvulus (p = .048) were associated with reduced survival to hospital discharge.ConclusionsHorses with AIH had a higher risk of concurrent SI volvulus and lower survival than reported. Draft breeds, high heart rate on admission and concurrent SI volvulus were associated with reduced short‐term survival.Clinical significanceThe results of this study should help in prognostication for horses with AIH.
Electrochemical Performance of Co1-xMnxFe2O4 Decorated Nanofiber Polyaniline Composites
Authors: Mohammed Sura R. , Ismail Mukhlis M. , Ibrahim Isam M. 
Published: August 2023

Abstract
The current work is concerned with preparing cobalt manganese ferrite (Co1-xMnxFe2O4) with different concentrations of cobalt and manganese (x = 0.2, 0.4, and 0.6) and decorating it with polyaniline (PAni) for use in supercapacitive applications. The results of the X-ray diffraction (XRD) manifested a broad peak of PAni and a cubic structure of cobalt manganese ferrite having crystal size between 60 nm and 138 nm, which decreases with increasing concentration of Mn. The field emission scanning electron microscopy (FE-SEM) images evidenced that the PAni has nanofiber (NF) structures, according to the method of preparation, where the hydrothermal method was used. The magnetic properties of the prepared ferrite, as well as the prepared PAni/Co1-xMnxFe2O4 composites, were studied through the vibrating sample magnetometer (VSM) analysis, where the magnetic hysteresis loops of ferrite elucidated a significant influence on the manganese content and the decorated PAni, through the decrease of both saturation magnetism (Ms) and remnant magnetism (Mr) in addition to the corrosive field (Hc). Increasing the content of manganese in the composites led to an improvement in the energy storage performance of the capacitors, which were tested in 1 M of H2SO4 by using the cyclic voltammetry analysis, galvanostatic charge–discharge (GCD), and electrochemical impedance spectroscopy (EIS). Increasing the manganese content caused an increase in the specific capacity and a significant increase in the charging and discharging time; the highest capacitance is 556 F/g.
Characterization of Carbon Dioxide Hydrate Growth Kinetics in Carbon Micron Tube Oil–Water System
Authors: Lv Xiaofang , Ni XingYa , Zhao Yi , Liu Tianhui , Jing Shu , Bai Boyu , Liang Shangbin , Liu Yang , Ma Qianli , Wang Chuanshuo , Zhou Shidong 
Published: August 2023

Abstract
Carbon nanotubes have a significant impact on hydrate formation. However, the effect and mechanism of carbon micrometer tubes, which have a similar structure to carbon nanotubes, on the promotion of hydrate growth is not yet clear. Therefore, in this paper, experiments on the growth kinetics of CO2 hydrate in oil–water systems under the effect of multiwalled carbon microtubes (MWCMTs) were carried out. The effects of pressure, temperature, and oil–water ratio on the induction period and gas consumption of CO2 hydrate were investigated. It also revealed the hydrate growth promotion mechanism of MWCMTs. The conclusions were as follows: (1) MWCMTs could significantly improve the hydrate gas storage capacity in an oil–water system by up to 80.3% over the pure water system. (2) Pressure and temperature had a large effect on the storage capacity and induction time of CO2 hydrate, and the results showed that the induction time decreased significantly with increasing pressure and decreasing temperature. At the same time, the hydrate growth time was significantly shortened, but the gas storage capacity first increased and then decreased. One reason for this was that the hydrate film hindered gas–water mass transfer, and the other was that the gas dissolved by the oil droplets rapidly generated hydrates and could not continue to transfer gas molecules. (3) In the oil–water system, lipophilic MWCMTs carried adsorbed CO2 to contact water, at the same time providing a large number of hydrate nucleation sites to promote hydrate formation.
Biological hydrogen cyanide emission globally impacts the physiology of both HCN-emitting and HCN-perceiving
Pseudomonas
Authors: Anand Abhishek , Falquet Laurent , Abou-Mansour Eliane , L'Haridon Floriane , Keel Christoph , Weisskopf Laure 
Published: August 2023

ABSTRACT

Bacterial volatile compounds have emerged as important chemical messengers between bacteria themselves as well as in their interactions with other organisms. One of the earliest examples of bioactive volatiles emitted by bacteria is hydrogen cyanide (HCN), which was long considered a mere respiratory toxin conferring competitive advantage to cyanide-producing strains. Using cyanide-deficient mutants in two
Pseudomonas
strains and global transcriptome analysis, we demonstrate that the impact of HCN is much more global than previously thought. We first observed that the lack of cyanogenesis (i.e., the ability to produce HCN) in emitting strains led to massive transcriptome reprogramming affecting diverse traits such as motility and biofilm formation (respectively inhibited vs promoted by HCN), or the production of siderophores, phenazines, and other antimicrobial compounds (repressed by HCN). We then exposed non-cyanogenic strains to biogenically emitted HCN from neighboring cells and observed similar transcriptome modulations and phenotypic changes, suggesting that HCN not only acts endogenously but also exogenously, remotely manipulating important traits involved in competition and virulence, e.g., siderophore production, in other organisms. Cyanogenesis in
Pseudomonas
has long been known to play a role in both the virulence of opportunistic pathogens and the efficient biocontrol activity of plant-beneficial strains; however, this impact was so far thought to occur solely through the inhibition of respiration. We demonstrate here new ecological roles for a small and fast-diffusing volatile compound, which opens novel avenues in our understanding of and ability to interfere with important processes taking place in pathogenic and beneficial
Pseudomonas
strains.

IMPORTANCE

Bacteria communicate by exchanging chemical signals, some of which are volatile and can remotely reach other organisms. HCN was one of the first volatiles discovered to severely impact exposed organisms by inhibiting their respiration. Using HCN-deficient mutants in two
Pseudomonas
strains, we demonstrate that HCN’s impact goes beyond the sole inhibition of respiration and affects both emitting and receiving bacteria in a global way, modulating their motility, biofilm formation, and production of antimicrobial compounds. Our data suggest that bacteria could use HCN not only to control their own cellular functions, but also to remotely influence the behavior of other bacteria sharing the same environment. Since HCN emission occurs in both clinically and environmentally relevant
Pseudomonas
, these findings are important to better understand or even modulate the expression of bacterial traits involved in both virulence of opportunistic pathogens and in biocontrol efficacy of plant-beneficial strains.
Complete genome sequence of a human bacteremia isolate of
Kalamiella piersonii
Authors: Howard Mondraya , Maki Joel J. , Connelly Sara , Hardy Dwight J. , Cameron Andrew 
Published: August 2023

ABSTRACT

The complete genome of
Kalamiella piersonii
clinical isolate URMC-2103A041 from human bacteremia was determined using the hybrid assembly of short- and long-read sequencing chemistry. The genome contains a 3.93 Mb chromosome, three circular plasmids, and one linear plasmid.