bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tuftelin1 Drives Experimental Pulmonary Fibrosis Progression by Facilitating Stress Fiber Assembly
Authors: Niu Caoyuan , Xu Kai , Hu Yanan , Jia Yanling , Pan Xiaoyue , Wan Ruyan , Lian Hui , Wang Qiwen , Wang Lan , Yang Juntang , Li Yajun , Rosas Ivan , Yu Guoying 
Published: September 2023

Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease (ILD) with unknown etiology, characterized by sustained damage repair of epithelial cells and abnormal activation of fibroblasts. However, the underlying mechanism of the disease remains elusive. This study was designed to evaluate the role of Tuftelin1 (TUFT1) in IPF and elucidate its molecular mechanism. We investigated the level of TUFT1 in the IPF and bleomycin-induced mouse models and explored the influence of TUFT1 deficiency on pulmonary fibrosis. Additionally, we explored the effect of TUFT1 on cytoskeleton and illustrated the relationship between stress fiber and pulmonary fibrosis. Our results demonstrated a significant upregulation of TUFT1 in IPF and the bleomycin-induced fibrosis model. Disruption of TUFT1 exerted inhibitory effects on pulmonary fibrosis in both in vivo and in vitro settings. Moreover, TUFT1 facilitated the assembly of microfilaments in A549 and MRC-5 cells, with a pronounced association between TUFT1 and N-WASP observed during microfilament formation. Meanwhile TUFT1 can promote the phosphorylation of tyrosine residue 256 (Y256) of the Neuronal Wiskott-Aldrich syndrome protein (pY256N-WASP). Furthermore, TUFT1 promoted TGF-β1 induced fibroblast activation by increasing nuclear translocation of pY256N-WASP in fibroblasts, while wiskostatin, a N-WASP inhibitor, suppressed these processes. Our findings suggested TUFT1 plays an important role in pulmonary fibrosis via its influence on stress fiber, and blockade of TUFT1 effectively reduces pro-fibrotic phenotypes. Pharmacological targeting of the TUFT1-N-WASP axis may represent a promising therapeutic approach for pulmonary fibrosis.
Analysis and validation of genes joint expression in Crohn's disease and cervical cancer based on GEO database.
Authors: ZHANG Le , Zou Jing , Wang Zhen , Shu Lindan , Jiang Bei , Liang Yi , ZHANG YuZhe 
Published: September 2023

Abstract
Background: In recent years, numerous studies have demonstrated an increased incidence of cervical cancer in women with Crohn's disease (CD). This paper aims to delve into the underlying mechanism of this association.
Methods: Gene expression profiles for Crohn's disease (GSE95095 and GSE186582) and cervical cancer (GSE63514 and GSE63678) were obtained from the GEO database. Heterozygotes (DEGs) were searched for in experimental and control groups for both diseases. Gene modules for Crohn's disease and cervical cancer were also analyzed using the WGCNA method. Machine learning (LASSO logistic regression algorithm & RF method) was applied to screen the characterized genes in the two diseases. And the transcription factors related to the characterized genes were predicted. Finally, it was validated by Western Blot (WB) and immunohistochemistry experiments.
Results: From the pool of differential genes in both disease groups, we identified a total of 60 co-expressed genes. Using the WGCNA method, we found 11 key modular genes that were common to both diseases. Machine learning screening allowed us to identify a shared biomarker for both diseases: CXCR4. Furthermore, we predicted MYC as its transcriptional regulator. Finally, to validate our findings, we conducted immunohistochemistry and protein immunoblotting experiments, which confirmed that CXCR4 exhibits a higher expression level in cervical cancer.
Conclusion: This study screened a gene co-expressed in Crohn's disease and cervical cancer based on machine learning: CXCR4, which is expected to be a potential biomarker for both diseases.
New Insights in the Distribution Profile of TRPC3 and TRPC5 in the Mouse and Human Kidney
Authors: Diebolt Coline M. , Schaudien Dirk , Junker Kerstin , Krasteva-Christ Gabriela , Tschernig Thomas , Englisch Colya N. 
Published: September 2023

Abstract
Several reports previously investigated Transient Receptor Potential Canonical subfamily channels 3 and 5 (TRPC3/TRPC5) in the kidney. However, most of the conclusions are based on animal samples or cell cultures leaving the door open for human tissue investigations. Moreover, results often disagreed among investigators. Histological description is lacking since most of these studies focused on functional aspects. Nevertheless, the same reports highlighted the potential differing key-roles of TRPC3 or TRPC5 in various renal disorders. Hence, our interest to investigate the localization of TRPC3 and TRPC5 in human kidneys. For this purpose, both healthy mouse and human kidney samples that were originated from tumor nephrectomies have been prepared for immunohistochemical staining using knockout-tested antibodies. Blocking peptides confirmed antibody specificity. A normalized weighted diaminobenzidine (DAB) area score between 0–3 comparable to a pixelwise H-score was established and employed for semiquantitative analysis. Altogether, our results suggest that glomeruli only express little TRPC3 and TRPC5 compared to several segments of the tubular system. Cortical and medullary proximal tubules are strongly stained. Intermediate tubules, however, are only weakly stained. The distal tubule was studied in three different localizations and depending on species and primary antibody, the staining was marked although slightly varying throughout the different localizations. Finally, the collecting duct was independently of primary antibody more stained in human compared to mouse tissue. We provide evidence that TRPC3 and TRPC5 are differently expressed in various localizations in both mouse and human samples. Especially, the TRPC5 distribution profile, we present here is completely new to our knowledge and raises questions, for instance its physiological relevance in the tubular system. We less verify results of previous studies than propose until now undescribed localizations of TRPC3 and TRPC5 in the mouse but especially and of greater interest in the human kidney. We thereby not only support the translational concept of TRPC channels as key players in physiology and pathophysiology of the human kidney but also present new potential targets to functional analysis.
Application of fractional exhaled nitric oxide and nasal nitric oxide in the evaluation of asthma control
Authors: Ma Yucong , Lin Wenying , Zheng Haoqi , Wang Yang , Cui Jingjing , Liu Li 
Published: September 2023

Abstract
Background
Asthma is the most common chronic respiratory disease in childhood. Persistent chronic airway inflammation is a pathological feature of asthma, so the control of airway inflammation is particularly important in asthma treatment. In recent years, fractional exhaled nitric oxide (FeNO) and fractional nasally exhaled nitric oxide (FnNO) has been used in the evaluation of airway inflammation.
Methods
This was a retrospective study. This study retrospectively analyzed the clinical data of 88 patients with asthma who received treatment and follow-up at The First Hospital of Jilin University from 2019 to 2022. The inclusion criteria were as follows: a. all patients met the criteria for diagnosis of bronchial asthma14; b. chronic persistent asthma was treated with standard protocols for more than 3 months; and c. the patient was able to cooperate with lung ventilation function tests and FeNO and FnNO examination.All subjects were divided into a control group, a partial control group and a poor control group according to Childhood Asthma Control Test (C-ACT) scores. FeNO, FnNO, and pulmonary function were compared among the three groups. We also analyzed whether FeNO and FnNO were correlated with various indices of pulmonary function in patients with asthma.
Results
The level of asthma control was not affected by rhinitis attacks. The FeNO and FnNO values of the poor control group were significantly higher than those of the partial control group and the control group. As asthma control improved, forced vital capacity (FVC) showed a statistically significant increase. The FEV1, maximal expiratory flow at 50% of the FVC (MEF50%) and maximal midexpiratory flow (MMEF) levels in the poor control group were significantly lower than those in the partial control group and the control group, and the PEF level of the poor control group was significantly lower than that of the control group. FeNO and FnNO had no significant correlation with pulmonary function parameters.
Conclusions
FeNO and FnNO should be used to assess the level of control in patients with asthma. There is no significant correlation among FeNO, FnNO and various parameters of pulmonary function.
Density Functional Theory calculations for Graphene Oxide, Zinc Oxide and Graphene oxide/zinc oxide composite structure
Authors: Hattab Ahmed Hamdi , Tapabashi Nashwan Omar , Khalil Najla Jalil 
Published: September 2023

Abstract
Graphene oxide is a complex substance that possesses significant implications in both theoretical and practical domains. In order to examine the potential of graphene oxide (GO) in reducing the high band gap of conducting materials, the electronic properties, including topography and band gap, the materials were assessed utilising density functional theory (DFT). calculations. The “B3LYP” technique was employed, along with the “6-31G” (d, p) and “LanL2DZ” basis sets. The quantum chemical parameters that have been calculated and found to be connected with reduced efficiency include total energy (E), highest occupied molecular orbital energy (EHOMO), lowest unoccupied molecular orbital energy (ELUMO), energy gap (EH−L), hardness (η), softness (S), and global electrophilicity index (ω). Applying the abbreviated Fukui function and abbreviated softness indicators facilitated the evaluation of potential regions for local reactivity. The results show that the total energy E is the highest at GO/ZnO composite which mean that it the most stable compound. While the EH−L for the composite was about 1.62 and this can prove the evidence that the composite is more relabel for the photo degradation than the ZnO in visible light.
Tuberculosis Reactivation Demonstrated By Choroiditis And Inflammatory Choroidal Neovascular Membrane In A Patient Treated With Immune Checkpoint Inhibitors For Malignant Mucosal Melanoma
Authors: Murphy Melissa Louise , Rogers Duncan 
Published: September 2023

Abstract
Purpose
To describe a complex case of ocular tuberculosis reactivation with anterior uveitis, choroiditis and inflammatory choroidal neovascular membrane (CNVM) following immune checkpoint inhibitor (ICPI) treatment of malignant mucosal melanoma.
Methods
A retrospective collection of medical history, clinical findings and multimodal imaging with literature review of the topic was conducted.
Results
A 52-year-old Romanian female developed reduced vision and photophobia after three cycles of ICPI therapy comprised of ipilimumab and nivolumab. Bilateral anterior uveitis, multiple left eye choroidal lesions and a CNVM were confirmed using slit-lamp examination with appropriate multimodal imaging. Retinal changes in the right eye as well as a history of previously treated posterior uveitis and high-risk ethnicity increased clinical suspicion for tuberculosis (TB) ocular reactivation. The diagnosis was confirmed by TB positivity on polymerase chain reaction (PCR) analysis of lung aspirate followed by significant clinical improvement on systemic anti-tubercular therapy (ATT), systemic steroids and anti-vascular endothelial growth factor (VEGF) therapy.
Conclusions
ICPIs can cause a myriad of ocular issues, both by primary immunomodulatory effects as well as secondary reactivation of latent disease.
Quality Indicators for Hospital Burn Care: A Scoping Review
Authors: Suzuki Denise R. Rabelo , Santana Levy Aniceto , Ávila Juliana Elvira H. Guerra , Amorim Fábio Ferreira , Modesto Guilherme Pacheco , Gotems Leila Bernarda Donato , Maldaner Vinicius 
Published: September 2023

Abstract

Background
The treatment of severe burns requires the management of a specialized multidisciplinary team and the coordination of several hospital services. Evaluating the quality of care provided to acute burn patients through quality indicators makes it possible to develop and implement measures aiming at better results. There is a lack of information on which indicators evaluate care in burn patients. The purpose of this scoping review was to identify a list of quality indicators used to evaluate the quality of hospital care provided to acute burn patients and indicate the gaps in current knowledge.
Method
A comprehensive scoping review (PRISMA-ScR) was conducted in four databases (PubMed, Cochrane Library, Embase, and Lilacs/VHL). Potentially relevant articles were evaluated for eligibility. General data and the identified quality indicators were collected for each included article. Each indicator was classified as a structure, process, or outcome indicator.
Results
A total of 1548 studies were identified, 82 were included, and their reference list was tracked, adding 19 more. Thus, data were collected from 101 studies. This review identified eight structure quality indicators, 72 process indicators, and 19 outcome indicators listed and subdivided according to their objectives.
Conclusion
This study obtained a list of quality indicators already used to monitor and evaluate the hospital care of acute burn patients, which may be useful for other research or be used in quality improvement programs.
Study Registration:
Protocol was registered on the Open Science Framework platform on June 27, 2022 (https://doi.org/10.17605/OSF.IO/NAW85).
Urinary Catheter-Directed Breast-Conserving Surgery for Incidentally Discovered Breast Cancer After Vacuum-assisted Breast Biopsy (VABB) Therapeutic Excision
Authors: Lu Yi , Qin Chuan , Zhou Chenghao , Cao Feilin , Li Zhanwen , Xie Xiaohong , Hu Guinv , Shen Jun , Zhou Yulu , Chen Cong , Li Zhaoqing , Sohail Ayesha , Singh Shreya , Zhou Jichun , Wang Linbo 
Published: September 2023

Abstract
Purpose
Vacuum-assisted breast biopsy (VABB) offers high accuracy in the diagnosis of breast tumors but makes the location of the original lesion difficult to identify when the subsequent breast-conserving surgery (BCS) is to be carried out. The aim of this study was to describe an effective technique to identify the exact location of the VABB cavity and to make breast-conserving resection more precise.
Methods
In this study, a balloon urinary catheter was inserted through the biopsy track; thus, the inflated balloon and catheter perfectly reconstructed the VABB cavity and biopsy track. Then breast-conserving resection with the guidance of the catheter was performed. Six patients were enrolled and assessed for their surgical findings, pathological results, surgical margins, and postoperative complications.
Results
Residual tumors were confirmed in 4 out of 6 patients after VABB. All the patients gained negative margins and underwent successful breast-conserving surgery. None of them required re-excisions. Satisfactory cosmetic results were observed after the catheter-directed BCS.
Conclusions
The results of this study show the effectiveness of urinary catheter-guided breast-conserving surgery after VABB. The procedure is simple and inexpensive. This new technique enabled precise breast-conserving resection of the targeted lesion and potentially improve successful rate of breast-conserving surgery.
A new definition of generalization of the factorial function with new results about the gamma of negative integers
Authors: Hussein Mohammed 
Published: September 2023

Abstract
The gamma function is a mathematical function that generalizes the concept of factorial to real and complex numbers. While the gamma function is a powerful tool in mathematics, it does have certain limitations and potential issues for example,
• Non-integer values: The gamma function is not defined for negative integers, this limitation can be problematic in certain contexts where negative integer values are involved.
• Pole at zero: The gamma function has a pole at zero, which means it is undefined at this point. This can pose challenges when working with functions or equations that involve the gamma function near or at zero.
• Computational complexity: Computing the gamma function numerically can be
computationally expensive and time-consuming, especially for large or complex arguments.
In this study, we have addressed the aforementioned issues by proposing a new definition for generalizing the factorial function, which serves as an alternative definition of the gamma function. This new definition is formulated based on the utilization of the differential operator.
The resulting definition stands out for its simplicity and effectiveness in computing real numbers, including non-positive integers. Moreover, our research has yielded fresh insights into the gamma function's behavior with respect to non-positive integers, potentially leading to a transformative approach in employing fractional differential and integral equations to describe a wide range of cosmic phenomena.
Misjudging drinking water quality risk: Adopting a barrier approach for meaningful risk assessment to address latent risks
Authors: Walker Richard 
Published: September 2023

Abstract
The water quality risk assessment is the foundation for every drinking Water Safety Plan. The need to conform with a ‘Corporate’ risk assessment model commonly dominated by asset management frequency data can cause misjudgement of microbial risk. Well-performed risk assessments should identify potential risks. Risk assessments which place too much emphasis on historical evidence to demonstrate ‘likelihood’ of microbial contamination are unsuitable for drinking water quality because they fail to recognise ‘latent’ risks associated with absent or underperforming barriers to contamination. Most outbreaks occur when these ‘latent’ factors align to create a failure. Good risk assessments need to provide foresight. This is achieved if drinking water quality risk assessments are based on a ‘barrier’ approach. Where adequate and reliable multiple barriers to contamination are present the likelihood of a hazardous event should then be categorised as rare. Where barriers are absent, inadequate, or unreliable, then a higher likelihood is appropriate, depending on the nature and extent of the barrier shortfall. Practical examples show how the ‘barrier’ approach can be applied. The barrier risk assessment directly informs the operational monitoring programme, enabling regular confirmation that the challenge and barrier performance are consistent with the predictions of the risk assessment.
Analysis on influence of consolidation and settlement of deep silt soil on existing pipeline – take the project of the fourth water supply pipeline to Macao as an example
Authors: Song Jian , Zhao Xuguang , Xu Jin , Shang Zheng , Liu Dian , Pan Haijie 
Published: September 2023

Abstract
As time goes by in deep silt, the water in the shallow layers of the soil and the water pressure will both dissipate, causing greater deformation of the soil structure. Based on the analysis of the new pipeline in Zhuhai, the post-settlement of the existing pipeline in the silt is calculated by theoretical analysis and simulation. It is concluded that the displacement deformation of the water supply pipeline after 400 days of sediment consolidation is still in safety control and puts forward some optimization methods to ensure the safety and function of the pipeline and provide the guidance basis for the follow-up maintenance and construction. It has a certain practical value.
Three Decades of Targeting Falcipains to Develop Antiplasmodial
Agents: What have we Learned and What can be Done Next?
Authors: González Jorge Enrique Hernández , Salas-Sarduy Emir , Alvarez Lilian Hernández , Valiente Pedro Alberto , Arni Raghuvir Krishnaswamy , Pascutti Pedro Geraldo 
Published: September 2023

Abstract:
Malaria is a devastating infectious disease that affects large swathes of human
populations across the planet’s tropical regions. It is caused by parasites of the genus
Plasmodium, with Plasmodium falciparum being responsible for the most lethal form of
the disease. During the intraerythrocytic stage in the human hosts, malaria parasites multiply
and degrade hemoglobin (Hb) using a battery of proteases, which include two cysteine
proteases, falcipains 2 and 3 (FP-2 and FP-3). Due to their role as major hemoglobinases,
FP-2 and FP-3 have been targeted in studies aiming to discover new antimalarials
and numerous inhibitors with activity against these enzymes, and parasites in culture
have been identified. Nonetheless, cross-inhibition of human cysteine cathepsins remains
a serious hurdle to overcome for these compounds to be used clinically. In this article,
we have reviewed key functional and structural properties of FP-2/3 and described different
compound series reported as inhibitors of these proteases during decades of active research
in the field. Special attention is also paid to the wide range of computer-aided
drug design (CADD) techniques successfully applied to discover new active compounds.
Finally, we provide guidelines that, in our understanding, will help advance the rational
discovery of new FP-2/3 inhibitors.
Morphine-like Novel Compounds for the Treatment of Depressive Symptoms
Authors: Choudhary Diksha , Kaur Rajwinder , Kumar Bhupinder 
Published: September 2023

:
Depression is a severe mental disorder characterized by a major imbalance between the levels of neurotransmitters. US11534436 B2 discloses an efficient amount of µ-opioid receptor agonists or their pharmaceutically acceptable salt for efficient treatment of depression. The patent discloses a few new compounds among which compounds 1, 2, 3, 4, and 5 as well as derivatives of basic nucleus A have good µ-opioid receptor agonist activity without risks of opioid addiction or development of addiction withdrawal symptoms. The compounds displayed Emax of 5% to 45% in a GTPγS binding assay while one of the derivatives displayed Emax of 15% to 35% in a GTPγS binding assay. The patent further discloses different embodiments with different potentials. The administration of an effective dose in rats resulted in 125% to 300% efflux of dopamine of baseline. At a dosage of 1-10 mg/kg, the embodiments do not diminish the thermal pain in rodents. Although the patent explored new µ-opioid receptor agonists for the treatment of depressive symptoms, the exhaustive evaluation of toxicity and further mechanisms need to be explored via in vivo models.
Diverse Pharmacological Potential of Pyridazine Analogs against Various Diseases
Authors: Alsaiari Ahad Amer , Almehmadi Mazen M. , Asif Mohammad 
Published: September 2023

Abstract:Pyridazinone analogs possess diverse types of pharmacological activities, such as anticancer, antimicrobial, anticonvulsant, analgesic, anti-inflammatory, antioxidant, antihypertensive, antisecretory, antiulcer, and other useful pharmacological activities. They also possess cyclooxygenase (COX) inhibitors, dipeptidyl peptidase inhibitors, phosphodiesterase inhibitors, glutamate transporter activators, adenosine receptor antagonists, serotonin receptors antagonists, lipooxygenase, cholinesterase, vasodilator, and anesthetics. Pyridazine rings are the essential structure for some marketed drugs, such as pimobendan, levosimendan as a cardiotonic drug, and emorfozan as an analgesic and anti-inflammatory (Non-steroidal anti-inflammatory drug) agent. So, researchers all over the world have paid attention to synthesizing various pyridazinone compounds mainly due to the ease of design and synthesis of different analogs and variables in the pharmacological responses. This review article focuses on the pharmacological activities of different pyridazine analogs.