bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emission, sequestration, credit and trading of carbon under gamhar (Gmelina arborea Roxb.) based agroforestry system towards net zero carbon emission of India
Authors: Kumar Abhay , Shabnam Swati , Kumar Rakesh , Karmakar S. , Das Soumitra Sankar , Lakra Kerobim , Singh Indra , Kumar Rikesh , Sinha Asha Kumari , Barla Sheela , Kumari Nargis , Prasad Muneshwar , Hasan Wajid , Mahto Dinesh , Kumar Jitendra , Malik M.S. , Oraon P.R. 
Published: August 2023

Abstract
Currently 22% (13Gt CO2-eq) of the total global greenhouse gas emission is contributed through agriculture, forestry and other land use. In current global scenario of environmental goals, farming sector is under tremendous pressure of reducing its share of emissions. Agroforestry system is the best option to achieve net zero carbon emissions target of India. Keeping this view, to evaluate the intercrops and gamhar based agroforestry system for their biomass production along with carbon emission, carbon sequestration, carbon credit, and carbon trading potential. The experiment was laid out in randomized block design with seven different treatments and five replications Gamhar tree biomass accumulation was higher in gamhar based agroforestry system compared to sole gamhar. Among different tree components, stem contributed a maximum to total gamhar tree biomass followed by roots, leaves and branches. Average contribution of stem, roots, leaves and branches in total tree biomass was 50.00, 19.79, 19.18 and 10.73 percent in 2016-17, 59.94, 19.98, 12.74 and 7.27 percent in 2017-18, and 58.05, 20.00, 14.02 and 7.93 percent in pooled data, respectively. In case of crops, above ground, below ground and total biomass was significantly higher in sole intercrops than under gamhar based agroforestry system. Total (Tree + interrops + Soil) carbon stock, carbon sequestration, carbon credit and carbon price were significantly affected by treatments, and was maximum in Sole Greengram-Mustard. Net carbon emission was also recorded lowest in Sole Greengram-Mustard for which the values were 811.55% and 725.24% and 760.69% lower than Sole Gamhar in 2016-17, 2017-18 and in pooled data, respectively.
The Joint Effect of Body Mass Index and Serum Lipid Levels on Incident Dementia among Community-dwelling Older Adults
Authors: Lei Qiqi , Xiao Zhenxu , Wu Wanqing , Liang Xiaoniu , Zhao Qianhua , Ding Ding , Deng Wei 
Published: August 2023

Abstract
Objective: This study aimed to explore the joint effect of body mass index (BMI) and serum lipids levels on incident dementia.
Methods:We prospectively followed up with 1,627 dementia-free community residents aged ≥60 for 5.7 years on average. At baseline, weight,and height were measured, and total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were detected in serum. Demographic characteristics were collected through questionnaires. Dementia was based on consensus diagnosis of neurologists and neuropsychologists using DSM-IV criteria. A generalized additive model (GAM) was used to assess the exposure-response relationship between BMI and serum lipid levels and dementia risk. Interactions and further classifications of BMI and serum lipid levels were further presentedby bivariate surface models and decision-tree models.
Results: The joint effects of TC with BMI, TG with BMI, and LDL-C with BMI on the risk of incident dementia shared a similar pattern, which was different from their independent exposure-response curves. The joint effect of HDL-C with BMI showed an S-surface but without statistical significance. Participants withTC<5.4 andBMI<21 (Hazard Ratio(HR) 1.93, 95% Confidence Interval (CI) 1.05-3.53), TC<5.4 and BMI≥21 (HR 1.73, 95% CI 1.09-2.72), and TC≥5.4 and BMI<21 (HR 4.02, 95% CI 2.10-7.71) were identified to have the increased risk of incident dementia compared to those with TC≥5.4 andBMI≥21. Participants with TG<1.7 and BMI<21 had an increased risk of incident dementia compared to those with TG≥1.7 and BMI≥21 (HR 1.98, 95%CI 1.17-3.3). Participants with LDL-C≥3.3 andBMI<21 were identified to have an increased risk of incident dementia compared to those with LDL-C≥3.3 and BMI≥21 (HR 3.33, 95%CI 1.64-6.78).
Conclusions: Our study showed that low BMI combined with low or high levels of serum lipids may increase the risk of dementia among older adults. This finding suggests the potential impacts of these two metabolic indexes on the risk of dementia.
Weed Ecological Attributes as Indicators of Sustainability in Agroecosystems of the Southeastern Region of Mexico City
Authors: Rendón-Aguilar Beatriz , Velázquez-Cárdenas Xenia Mitzi Yetlanezi , Morales-Gutiérrez Karla , Rivera-Ramírez Ismael , Camero-Aguilar David , de Santiago-Gómez Jesús Ricardo 
Published: August 2023

Abstract

In Mexico City, 16% of the total land area is devoted to agricultural activities with evidence of pre-Hispanic heritage, characterized by the prevalence of various agroecosystems. In the middle of the 20th century, urbanization produced changes in agricultural activities. We analyzed contemporary aspects related to technological, socioeconomic, and cultural components of three agroecosystems and their relationship with some ecological and cultural parameters of weeds, considering them as "agroecosystem sustainable indicators". We conducted 179 interviews with campesinos in SUBURBAN and PERIURBAN spaces of the municipalities of Milpa Alta, Tláhuac, and Xochimilco, and selected 30 plots for sampling weeds. We recorded the abundance of each species and estimated different ecological attributes. We applied non-parametric and multivariate analysis to compare weed attributes between agroecosystems and spaces. Most of the collaborators are native from Mexico City, they are not full time campesinos, and they have changed some agricultural practices. Displacement of traditional crops and agricultural practices, and incorporation of others, has modified many ecological attributes of weeds. Chinampa was impacted the most, whereas slope was least affected. We recorded 156 species throughout the 30 samples. The slope had the highest diversity, and ciénega the lowest. More than half are native, where slope shared the highest number. Ecological Importance Value indicates that there are not dominant species in slope. Species composition also differs between the three agroecosystems. Slope is the most sustainable agroecosystem and must be use as a model to protect and recover the agrobiodiversity of the other agroecosystems of CDMX.
High-dose cytarabine induction therapy and flow cytometric measurable residual disease monitoring for children with acute myeloid leukemia
Authors: Tomizawa Daisuke , Matsubayashi Jun , Iwamoto Shotaro , Hiramatsu Hidefumi , Hasegawa Daisuke , Moritake Hiroshi , Hasegawa Daiichiro , Terui Kiminori , Hama Asahito , Tsujimoto Shin-ichi , Kiyokawa Nobutaka , Miyachi Hayato , Deguchi Takao , Hashii Yoshiko , Iijima-Yamashita Yuka , Taki Tomohiko , Noguchi Yasushi , Koike Kazutoshi , Koh Katsuyoshi , Yuza Yuki , Moriya-Saito Akiko , Horibe Keizo , Taga Takashi , Tanaka Shiro , Adachi Souichi 
Published: August 2023

Abstract
Japanese Pediatric Leukemia/Lymphoma Study Group AML-12 is a seamless phase II/III cooperative study to evaluate a role of high-dose cytarabine (HDAC) in initial induction therapy and significance of measurable residual disease (MRD) at end of induction therapies for children with acute myeloid leukemia (AML). From 2014 to 2018, a total of 359 patients were enrolled; 324 patients were randomly assigned to receive standard-dose cytarabine induction or HDAC induction. Following the common second induction, patients achieving remission were stratified into either of the three risk groups and received consolidation chemotherapy with or without allogeneic hematopoietic stem cell transplantation. MRD was centrally monitored at end of Induction-1 (TP-1) and end of Induction-2. There were no significant statistical differences in the primary endpoints between the two arms; MRD-positive (≥ 0.1%) rate at TP-1 (21.5% vs. 25.2%, P = 0.517) and 3-year event-free survival rate (64.3% [95%CI, 56.5% – 71.0%] vs. 61.2% [53.1% – 68.4%], P = 0.589). MRD could be measured for 262 (94.9%) of the 276 patients for whom MRD monitoring was attempted and was demonstrated to be the strongest factor to predict the outcomes. Although, HDAC in initial induction did not improve the outcomes of children with AML, flow cytometric MRD monitoring was feasible and highly prognostic.
Inhibition of TRPM3 by primidone provides a potential therapeutic method for adenomyosis management
Authors: Jin Zhixing , Peng Yaoming , Zhang He , He Xiaoping , Zhang Yi , Pan Xin , Li Min , Yang Qianqian 
Published: August 2023

Abstract
Purpose
To test the expression profile of transient receptor potential channels (TRPs) in adenomyosis patients and evaluate the effects of primidone on tamoxifen-induced adenomyosis mice.
Methods
Eutopic endometrium from adenomyosis patients (n = 20) was collected and subjected to mRNA analysis of TRP channels. TRPA1, TRPV1 and TRPM3 in adenomyosis patients (n = 50) and tamoxifen-induced adenomyosis mice (n = 6) were examined by immunohistochemistry. From 10 weeks after birth, primidone (2 mg/kg/d) and atosiban (1 mg/kg/d) were given separately to adenomyotic mice by intraperitoneal injection for 3 weeks. The hotplate test was conducted once a week beginning at 10 weeks, and then uterine samples were harvested for HE staining and RNA-seq at 13 weeks.
Results
The mRNA expression of 15 TRPs was significantly increased in the proliferative phase of the adenomyotic endometrium. TRPV1, TRPM3 or TRPA1 staining levels were positively correlated with dysmenorrhea severity, menses amount and uterine size. In tamoxifen-induced adenomyosis mice, primidone had a significant effect on both the depth of myometrial infiltration and analgesia. Forty-seven DEGs were identified after primidone treatment, and bioinformatics analysis predicted that they were enriched in the cell cycle and cell division.
Conclusion
The expression profile of TRP channels varies significantly in adenomyosis patients, and primidone may provide a potential therapeutic method for adenomyosis management.
Evaluation and genetic dissection of the powdery mildew resistance in 558 wheat accessions
Authors: Jin Yuli , Han Guohao , Zhang Wenjing , Gao Huiming , Bu Bin , Zhao Ya , Wang Jiaojiao , Liu Ruishan , Yang Hong , Ma Pengtao 
Published: August 2023

Abstract
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a devasting disease of wheat all over the world, leading to significant yield losses. Host resistance is the most cost-effective and eco-soundly strategy to control this disease. It is certainly significant to assess the resistance level of various wheat germplasms as well as identify their genetic basis for the resistance. In the present study, a total of 558 wheat accessions were evaluated their seedling resistance to powdery mildew and the presence of resistance genes Pm1, Pm2, Pm4, Pm5, Pm6, Pm8, Pm21, Pm24, Pm41, Pm60 and Pm69 using different Bgt isolates and gene specific molecular markers. The results indicated that only 25 out of 558 wheat accessions were resistant to the Bgt isolate E09, suggesting the overall level of powdery mildew resistance of these accessions is inadequate. Further, these 25 accessions showed remarkable different resistance spectrum to extra 25 Bgt isolates and ranged from single to 25 ones. Among them, seven accessions remained resistant to all the tested isolates, indicating these accessions possess genes with broad spectrum resistance. Subsequently, the molecular detection using closely linked/diagnostic marker results showed that 249 accessions could carry only one of the tested Pm genes, 75 carry combined Pm genes and 234 not carry any of tested genes. Pm8 was detected at the highest frequency of 61.45%, whereas Pm5, Pm24, Pm41 and Pm69 were not detected. Notably, for seven accessions resistant to all the 26 tested isolates, Pm21 was detected in four of these wheat accessions, including HengS29, Chang 4640, Xinong 556 and ML728; Pm2 and Pm60 were identified in Emai 18 and Xinmai 296, respectively; no any of tested genes were detected in Emai 16, indicating novel broad spectrum resistant gene(s) might be present in this accession. The information on the evaluation and genetic dissection of the powdery mildew resistance in these 558 wheat accessions can be valuable for the popularization of these accessions and also for the powdery mildew resistance breeding using these accessions.
Why is end-of-life inpatient cost high among cancer patients? A prospective cohort study
Authors: Balasubramanian Ishwarya , Malhotra Chetna , group COMPASS study 
Published: August 2023

Abstract
Background:
Inpatient cost for cancer patients is high during the last six months of life, but reasons for this are not understood. We aim to understand the type of hospital admissions and inpatient services associated with an increase in inpatient cost in last months of life.
Methods:
We used survey and billing records of 439 deceased patients with a solid metastatic cancer, enrolled in a prospective cohort study. Based on cost per day of inpatient admissions, we classified admissions as low- or high-intensity. We decomposed the inpatient cost into cost for different inpatient services. We assessed patient characteristics associated with higher inpatient cost.
Results:
Towards death, proportion of inpatient cost for ‘maintenance care’ increased while that for intensive care unit (ICU) and surgeries decreased. Low-intensity, compared to high-intensity admissions had a higher proportion of cost for ‘maintenance care’ and a lower proportion for surgeries and ICU. Number of low-intensity admissions increased more steeply towards death than high-intensity admissions. Both admission types contributed equally to the share of inpatient cost. Older patients were less likely to have a high-intensity admission (β: -0.01, CI: -0.02,-0.00). Greater preference for life extension (β: 0.06, CI: 0.01,0.12) and inaccurate prognostic belief were associated with higher cost of high-intensity admissions (β: 0.31, CI: -0.01,0.62).
Conclusions:
Findings suggest that inpatient costs in last six months of life may be reduced if maintenance care is availed in low-cost settings such as hospice/palliative care alongside steps to reduce non-beneficial surgeries and ICU admissions.
Trial registration
NCT02850640 (Prospectively registered on June 9, 2016)
Deep neural nets for Evaluation method of Table tennis athletes' psychological load intensity based on Artificial Intelligence
Authors: Qu Lian Zhu 
Published: August 2023

Abstract
Table tennis athletes are easily disturbed by the environment, psychology and other factors in the process of competition, and there is high uncertainty. Therefore, an artificial intelligence based evaluation method of Table tennis athletes' psychological load intensity is proposed. Based on the collection and monitoring of Table tennis athletes' psychological load data, this paper preprocesses them to complete the monitoring of Table tennis athletes' psychological load intensity. Based on this, from the factors that affect the psychological load of Table tennis athletes, this paper constructs an evaluation index system of Table tennis athletes' psychological load intensity including training pressure and environment. According to the index system, the psychological load intensity of Table tennis athletes is evaluated by fuzzy mathematical tools. The results show that the psychological load of the experimental Table tennis athletes is 6.45, which belongs to moderate load; Moreover, the evaluation method has a high accuracy in evaluating 8 items of Table tennis athletes' psychological load intensity, such as too long training time, too much training content and noise impact, which can effectively improve the training quality of Table tennis athletes.
Molecular characterization, phylogenetic analysis and viral load quantification of Lumpy Skin Disease Virus in Cattle
Authors: K Sanganagouda , Nagraja K , Sajjanar Basavaraj , Kounin Sabha , Gomes Amitha Rena , H Pavithra B , Lalasangi Sanjeevakumar , R Sumathi B , Murag Shivaraj , P Shankar B , R Anjan Kumar K , Kharate Arun , K Muniyelappa H , D Venkatesha M , Hegade Raveendra 
Published: August 2023

Abstract
Lumpy skin disease is an emerging, remerging and trans-boundary disease caused by lumpy skin disease virus (LSDV) belongs Capripoxvirus genus and Poxviridae family in cattle. The recent episodes of disease outbreak in last 4 years from 2019 to 2023 caused huge socio-economic loss. In the present study, clinical samples from LSD outbreaks were collected from different districts of Karnataka, India for virus isolation and molecular characterization. Preliminary confirmation of LSD virus was performed by targeting envelope protein p32 (192 bp) gene by conventional Polymerase chain reaction (PCR). Real-Time qPCR was developed for absolute quantification of viral copy numbers in the infected samples. The viral copy number Log10 (Mean ± SD) in majority of the representative samples ranged from 4.962 to 12.432. The partial genes of GPCR and P32 were sequenced and submitted to GenBank. Phylogenetic analysis was performed by comparing sequences with other LSDV, Sheep pox virus (SPPV) and Goat pox virus (GPPV). The results indicated that our isolates have similarity with the LSDV sequences from Nepal, Bangladesh, Botswana, Egypt, Kenya Turkey and Saudi Arabia. It was also found that one of our isolate (OQ383668) clustered with the GPPV with 100% similarity. Our results highlight the necessity of ongoing surveillance and molecular characterization of circulating LSDV strains required for perspective development of suitable LSD vaccine to control the disease.
Square beams for optimal tiling in TEM
Authors: de Marco Alex , Chua Eugene , Alink Lambertus , Kopylov Mykailo 
Published: August 2023

Abstract
Imaging large fields-of-view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large field-of-view is either imperfect or results in uneven exposures, which is a problem on dose-sensitive samples. Here we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application showing it can tile nearly perfectly and deliver cryo-EM imaging with a resolution comparable to conventional setups.
A feasibility study on the complementarity of quality and genetic tests on seed certification: lessons from the hybrid maize in Malawi
Authors: Hunga Henry Geoffrey , Soon-Sinclair Jan Mei 
Published: August 2023

Abstract

At $1.5 billion the African seed sector accounts for 3.5 per cent of the global seed market. The growth is attributed to economic reforms and African countries adopting market-oriented policies promoting the private sector role and regional integration. However, smallholder farmers have reported poor quality certified seeds on the market. Therefore, this feasibility study sought to prove the existence of counterfeit hybrid maize seeds on the market in Malawi. Using the mystery shopper approach 37 hybrid maize seed samples were bought from agro-dealers and eight reference seed samples from the parent seed companies in three districts of Mchinji, Dowa, and Lilongwe. The agro-dealers were categorised whether licenced or not using the Seed Trade Association database. This was followed by quality and genetic purity using simple sequence repeat (SSR) tests. Results show that only 34% of the agro-dealers where the seed samples were procured were licensed by the government. Quality tests showed that the seeds were within the acceptable range for germination, moisture content, and purity percentage. However, genetic variation results showed that only one of the 37 samples matched the reference seed sample and the rest of the samples exhibited heterozygosity traits not matching the reference samples or similar lines.
rhIFN-α14 changes skin microbiota in naturally occurring canine atopic dermatitis
Authors: Beirão Breno C. B. , Taraciuk Aline C. , Gadotti Ana Carolina , Klassen Elisa , Trentin Carolina , Ingberman Max , McKenzie Chris , Stimson William H. 
Published: August 2023

Abstract
Atopic dermatitis (AD) manifests as recurrent and progressive pruritic dermatitis in dogs. Treatment is lifelong and non-responders are common to most drugs, with the exception to glucocorticoids, which have many undesirable effects. Our group has previously shown clinical efficacy of recombinant human interferon-alpha14 (rhIFNα−14) in the treatment of naturally occurring canine atopic dermatitis. Here, we sought to verify the role of rhIFNα−14 in the dysbiosis of the skin during AD. Six dogs with chronic non-seasonal atopic dermatitis were selected and treated with the experimental compound (10,000 IU/kg of rhIFNα-14 orally for 8 weeks). Each dog was evaluated by a veterinarian once a week. Efficacy outcomes were based on a veterinary-conducted simplified version of the CADESI (canine atopic dermatitis extent and severity index) score. Owner assessment of the status of the dog was also collected during the clinical consultation. Blood samples were collected every fifteen days for haematological analysis. Skin microbiota was assessed by next-generation sequencing. Pruritus score was negatively correlated to bacterial family homogeneity, as were vet scores of excoriations and erythema. There were no associations between clinical scores with individual bacterial genera, including Staphylococcus spp. In conclusion, the administration of rhIFNα−14 positively changes the skin microbiota by increasing the bacterial family homogeneity and ameliorates the disease severity, indicating one possible mode of action of this cytokine in atopic dermatitis.
Ecological Niche Modeling for the Prediction of Suitable Habitat for Chrysodeixis chalcites (Noctuidae) in the Contiguous United States
Authors: Galle Nicholas A. , Hankins Kayla R. , Bucheli Sibyl R. , Williams Justin K. , Randle Christopher Patrick 
Published: August 2023

Abstract
The golden twin-spot moth, Chrysodeixis chalcites Esper (Lepidoptera: Noctuidae), is a polyphagous, polyvoltine crop pest occurring natively from northern Europe to Mediterranean Africa and the Canary Islands. Larvae feed on a wide variety of naturally occurring plants as well as soybean and other legume crops, short staple cotton, tomato, potato, peppers, tobacco, and banana. Chrysodeixis chalcites has been recorded in agricultural lands in the Ontario peninsula in eastern Canada and in northern counties of Indiana, USA. Given the strong potential for C. chalcites to invade USA crop lands, it is important to identify habitats most likely to sustain growing populations of this pest. Using occurrence data from its homerange, and environmental predictors including bioclimatic conditions, elevation, and human disturbance, we trained three ecological niche models, and used these models to estimate an ensemble prediction of habitat suitability in the contiguous US. Because human impact is potentially a confounding predictor, models were trained both with and without it. High habitat suitability was projected for the Atlantic coast from New England to Florida, the Gulf coast, the lower Midwest, and the Pacific coast and Central Valley of California. Though model predictions were robust, we recommend caution in their interpretation. First, agricultural lands are bioclimatically altered landscapes, and these alterations not reflected in bioclimatic data gathered from weather stations. Second, though the inclusion of human impact did not alter predictions on a large scale, it produced predictions favoring major metropolitan areas as suitable habitat, which we interpret as an artifact.