bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multivariate analysis and genetic dissection of staygreen and stem reserve mobilisation under combined drought and heat stress in wheat (Triticum aestivum L.)
Authors: Taria Sukumar , Arora Ajay , Krishna Hari , Manjunath Karthik Kumar , Meena Shashi , Kumar Sudhir , Singh Biswabiplab , Krishna Pavithra , Malakondaiah Animireddy China , Das Ritwika , Alam Badre , Kumar Sushil , Singh Pradeep Kumar 
Published: August 2023

Introduction: Abiotic stresses significantly reduce crop yield by adversely affecting many physio-biochemical processes. Several physiological traits have been targeted and improved for yield enhancement in limiting environmental conditions. Amongst them, staygreen and stem reserve mobilisation are two important mutually exclusive traits contributing to grain filling under drought and heat stress in wheat. Henceforth, the present study was carried out to identify the QTLs governing these traits and to identify the superiors’ lines through multi-trait genotype-ideotype distance index (MGIDI)Methods: A mapping population consisting of 166 recombinant inbred lines (RILs) developed from a cross between HD3086 and HI1500 was utilized in this study. The experiment was laid down in alpha lattice design in four environmental conditions viz. Control, drought, heat and combined stress (heat and drought). Genotyping of parents and RILs was carried out with 35 K Axiom® array (Wheat breeder array).Results and Discussion: Medium to high heritability with a moderate to high correlation between traits was observed. Principal component analysis (PCA) was performed to derive latent variables in the original set of traits and the relationship of these traits with latent variables.From this study, 14 QTLs were identified, out of which 11, 2, and 1 for soil plant analysis development (SPAD) value, leaf senescence rate (LSR), and stem reserve mobilisation efficiency (SRE) respectively. Quantitative trait loci (QTLs) for SPAD value harbored various genes like Dirigent protein 6-like, Protein FATTY ACID EXPORT 3, glucan synthase-3 and Ubiquitin carboxyl-terminal hydrolase, whereas QTLs for LSR were found to contain various genes like aspartyl protease family protein, potassium transporter, inositol-tetrakisphosphate 1-kinase, and DNA polymerase epsilon subunit D-like. Furthermore, the chromosomal region for SRE was found to be associated with serine-threonine protein kinase. Serine-threonine protein kinases are involved in many signaling networks such as ABA mediated ROS signaling and acclimation to environmental stimuli. After the validation of QTLs in multilocation trials, these QTLs can be used for marker-assisted selection (MAS) in breeding programs.
Dendritic cell-based vaccine prepared with recombinant Lactococcus lactis enhances antigen cross-presentation and antitumor efficacy through ROS production
Authors: Zhang Tingting , Wei Xianxian , Li Yijie , Huang Shuai , Wu Yulin , Cai Shanshan , Aipire Adila , Li Jinyao 
Published: August 2023

IntroductionLactococcus lactis (L.L) is safe and can be used as vehicle. In this study, the immunoregulatory effect of L.L on dendritic cell (DC) activation and mechanism were investigated. The immune responses and antigen cross-presentation mechanism of DC-based vaccine prepared with OVA recombinant L.L were explored.MethodsConfocal microscopy and flow cytometry were used to analyze the mechanism of L.L promoting DC maturation, phagosome membrane rupture and antigen presentation. The antitumor effect of DC vaccine prepared with L.L-OVA was assessed in the B16-OVA tumor mouse model.ResultsL.L significantly promoted DC maturation, which was partially dependent on TLR2 and downstream MAPK and NF-κB signaling pathways. L.L was internalized into DCs by endocytosis and did not co-localized with lysosome. OVA recombinant L.L enhanced antigen cross-presentation of DCs through the phagosome-to-cytosol pathway in a reactive oxygen species (ROS)- and proteasome-dependent manner. In mouse experiments, L.L increased the migration of DCs to draining lymph node and DC vaccine prepared with OVA recombinant L.L induced strong antigen-specific Th1 and cytotoxic T lymphocyte responses, which significantly inhibited B16-OVA tumor growth.ConclusionThis study demonstrated that recombinant L.L as an antigen delivery system prepared DC vaccine can enhance the antigen cross-presentation and antitumor efficacy.
A global unstructured, coupled, high-resolution hindcast of waves and storm surge
Authors: Mentaschi Lorenzo , Vousdoukas Michalis I. , García-Sánchez Guillermo , Fernández-Montblanc Tomás , Roland Aron , Voukouvalas Evangelos , Federico Ivan , Abdolali Ali , Zhang Yinglong J. , Feyen Luc 
Published: August 2023

Accurate information on waves and storm surges is essential to understand coastal hazards that are expected to increase in view of global warming and rising sea levels. Despite the recent advancement in development and application of large-scale coastal models, nearshore processes are still not sufficiently resolved due to coarse resolutions, transferring errors to coastal risk assessments and other large-scale applications. Here we developed a 73-year hindcast of waves and storm surges on an unstructured mesh of &gt;650,000 nodes with an unprecedented resolution of 2-4 km at the global coast. Our modelling system is based on the circulation model SCHISM that is fully coupled with the WWM-V (WindWaveModel) and is forced by surface winds, pressure, and ice coverage from the ERA5 reanalysis. Results are compared with observations from satellite altimeters, tidal gauges and buoys, and show good skill for both Sea Surface Height (SSH) and Significant Wave Height (Hs), and a much-improved ability to reproduce the nearshore dynamics compared with previous, lower-resolution studies. Besides SSH, the modelling system also produces a range of other wave-related fields at each node of the mesh with a time step of 3 hours, including the spectral parameters of the first three largest energy peaks. This dataset offers the potential for more accurate global-scale applications on coastal hazard and risk.
Association of C-type lectin-like receptor 2 and galectin-1 with portal vein system thrombosis in HBV-related liver cirrhosis
Authors: Zhang Yiyan , Zhang Xintong , Xu Xiangbo , Guo Xiaozhong , Xu Shixue , Ma Shaoze , Chen Jihong , Qi Xingshun 
Published: August 2023

Background and aimsHepatitis B virus (HBV) infection is the most common cause of liver cirrhosis. Portal venous system thrombosis (PVST) is a major complication of liver cirrhosis. Recently, it has been shown that C-type lectin-like receptor 2 (CLEC-2) and galectin-1 participate in the activation and aggregation of platelets, thereby promoting the development of thrombosis. This cross-sectional study aims to evaluate the association of serum CLEC-2 and galectin-1 levels with PVST in patients with HBV-related liver cirrhosis.MethodsOverall, 65 patients with HBV-related liver cirrhosis were included, of whom 23 had PVST and 42 did not have. Serum CLEC-2 and galectin-1 levels were measured using enzyme-linked immunosorbent assay kits. PVST was assessed by contrast-enhanced computed tomography and/or magnetic resonance imaging scans. Subgroup analyses were conducted according to the degree and location of PVST.ResultsPatients with PVST had significantly higher serum CLEC-2 (p = 0.006) and galectin-1 (p = 0.009) levels than those without. Patients with partial/complete PVST or fibrotic cord (p = 0.007; p = 0.002), but not those with mural PVST (p = 0.199; p = 0.797), had significantly higher serum CLEC-2 and galectin-1 levels than those without PVST. Patients with superior mesenteric vein thrombosis had significantly higher serum CLEC-2 (p = 0.013) and galectin-1 (p = 0.025) levels than those without PVST. Patients with main portal vein thrombosis had higher serum CLEC-2 (p = 0.020) and galectin-1 (p = 0.066) levels than those without PVST, but the difference in serum galectin-1 level was not significant between them.ConclusionSerum CLEC-2 and galectin-1 levels may be associated with the presence of PVST in HBV-related cirrhotic patients, but this association should be dependent upon the degree of PVST.
Associations between cognition, anxiety, depression, and residual dizziness in elderly people with BPPV
Authors: Sun Jin , Ma Xiaobao , Yang Ying , He Kuan , Wang Wei , Shen Jiali , Wang Lu , Chen Xiangping , Jin Yulian , Yang Jun , Chen Jianyong 
Published: August 2023

ObjectiveTo investigate the associations between cognition, anxiety, depression, and residual dizziness after successful repositioning maneuvers in the elderly with benign paroxysmal positional vertigo (BPPV).MethodsWe enrolled 40 elderly patients with BPPV in our outpatient department. We used the Dizziness Handicap Inventory (DHI), Visual Analog Scale (VAS), Patient Health Questionnaire-9 (PHQ-9), and Generalized Anxiety Disorder Questionnaire-7 (GAD-7) to assess the degree of dizziness, anxiety, and depression of participants before repositioning therapy, respectively. At the 1-week follow-up after BPPV treatment, each participant will be reassessed and divided into a group with residual dizziness (RD) and a group without residual dizziness (NRD) based on the follow-up DHI score. The Mini-Mental State Examination (MMSE) evaluated the cognitive function of the participants.ResultsThe age, gender, duration of BPPV, and involved semicircular canals in the two groups did not show a significant difference. The RD group scored significantly higher on the DHI (p = 0.006), GAD-7 (p &lt; 0.001), and PHQ-9 (p = 0.002) before the repositioning treatment than the NRD group. The two groups had no significant difference in MMSE score (p = 0.381). Anxiety and depression scores before repositioning treatment significantly and positively correlated with follow-up DHI scores (r = 0.678 and 0.522, respectively), but the MMSE score did not significantly relate to it. The univariate linear regression showed that the DHI (p &lt; 0.001), GAD-7 (p &lt; 0.001), and PHQ-9 (p = 0.002) scores before treatment could predict residual dizziness. The multivariate linear regression showed that GAD-7 before treatment was the only significant predictor of residual dizziness (p &lt; 0.001).ConclusionThe level of dizziness, anxiety, and depression before treatment can predict residual dizziness after successful repositioning maneuvers in the elderly with BPPV. Anxiety may be the strongest predictor of residual dizziness after successful repositioning treatment in elderly BPPV patients.
Hearing, seeing, and feeling speech: the neurophysiological correlates of trimodal speech perception
Authors: Hansmann Doreen , Derrick Donald , Theys Catherine 
Published: August 2023

IntroductionTo perceive speech, our brains process information from different sensory modalities. Previous electroencephalography (EEG) research has established that audio-visual information provides an advantage compared to auditory-only information during early auditory processing. In addition, behavioral research showed that auditory speech perception is not only enhanced by visual information but also by tactile information, transmitted by puffs of air arriving at the skin and aligned with speech. The current EEG study aimed to investigate whether the behavioral benefits of bimodal audio-aerotactile and trimodal audio-visual-aerotactile speech presentation are reflected in cortical auditory event-related neurophysiological responses.MethodsTo examine the influence of multimodal information on speech perception, 20 listeners conducted a two-alternative forced-choice syllable identification task at three different signal-to-noise levels.ResultsBehavioral results showed increased syllable identification accuracy when auditory information was complemented with visual information, but did not show the same effect for the addition of tactile information. Similarly, EEG results showed an amplitude suppression for the auditory N1 and P2 event-related potentials for the audio-visual and audio-visual-aerotactile modalities compared to auditory and audio-aerotactile presentations of the syllable/pa/. No statistically significant difference was present between audio-aerotactile and auditory-only modalities.DiscussionCurrent findings are consistent with past EEG research showing a visually induced amplitude suppression during early auditory processing. In addition, the significant neurophysiological effect of audio-visual but not audio-aerotactile presentation is in line with the large benefit of visual information but comparatively much smaller effect of aerotactile information on auditory speech perception previously identified in behavioral research.
Time to consider animal data governance: perspectives from neuroscience
Authors: Eke Damian , Ogoh George , Knight William , Stahl Bernd 
Published: August 2023

IntroductionScientific research relies mainly on multimodal, multidimensional big data generated from both animal and human organisms as well as technical data. However, unlike human data that is increasingly regulated at national, regional and international levels, regulatory frameworks that can govern the sharing and reuse of non-human animal data are yet to be established. Whereas the legal and ethical principles that shape animal data generation in many countries and regions differ, the generated data are shared beyond boundaries without any governance mechanism. This paper, through perspectives from neuroscience, shows conceptually and empirically that there is a need for animal data governance that is informed by ethical concerns. There is a plurality of ethical views on the use of animals in scientific research that data governance mechanisms need to consider.MethodsSemi-structured interviews were used for data collection. Overall, 13 interviews with 12 participants (10 males and 2 females) were conducted. The interviews were transcribed and stored in NviVo 12 where they were thematically analyzed.ResultsThe participants shared the view that it is time to consider animal data governance due to factors such as differences in regulations, differences in ethical principles, values and beliefs and data quality concerns. They also provided insights on possible approaches to governance.DiscussionWe therefore conclude that a procedural approach to data governance is needed: an approach that does not prescribe a particular ethical position but allows for a quick understanding of ethical concerns and debate about how different positions differ to facilitate cross-cultural and international collaboration.
Deviant spontaneous neural activity as a potential early-response predictor for therapeutic interventions in patients with schizophrenia
Authors: Jing Huan , Zhang Chunguo , Yan Haohao , Li Xiaoling , Liang Jiaquan , Liang Wenting , Ou Yangpan , Wu Weibin , Guo Huagui , Deng Wen , Xie Guojun , Guo Wenbin 
Published: August 2023

ObjectivePrevious studies have established significant differences in the neuroimaging characteristics between healthy controls (HCs) and patients with schizophrenia (SCZ). However, the relationship between homotopic connectivity and clinical features in patients with SCZ is not yet fully understood. Furthermore, there are currently no established neuroimaging biomarkers available for the diagnosis of SCZ or for predicting early treatment response. The aim of this study is to investigate the association between regional homogeneity and specific clinical features in SCZ patients.MethodsWe conducted a longitudinal investigation involving 56 patients with SCZ and 51 HCs. The SCZ patients underwent a 3-month antipsychotic treatment. Resting-state functional magnetic resonance imaging (fMRI), regional homogeneity (ReHo), support vector machine (SVM), and support vector regression (SVR) were used for data acquisition and analysis.ResultsIn comparison to HCs, individuals with SCZ demonstrated reduced ReHo values in the right postcentral/precentral gyrus, left postcentral/inferior parietal gyrus, left middle/inferior occipital gyrus, and right middle temporal/inferior occipital gyrus, and increased ReHo values in the right putamen. It is noteworthy that there was decreased ReHo values in the right inferior parietal gyrus after treatment compared to baseline data.ConclusionThe observed decrease in ReHo values in the sensorimotor network and increase in ReHo values in the right putamen may represent distinctive neurobiological characteristics of patients with SCZ, as well as a potential neuroimaging biomarker for distinguishing between patients with SCZ and HCs. Furthermore, ReHo values in the sensorimotor network and right putamen may serve as predictive indicators for early treatment response in patients with SCZ.
Deep-learning and conventional radiomics to predict IDH genotyping status based on magnetic resonance imaging data in adult diffuse glioma
Authors: Zhang Hongjian , Fan Xiao , Zhang Junxia , Wei Zhiyuan , Feng Wei , Hu Yifang , Ni Jiaying , Yao Fushen , Zhou Gaoxin , Wan Cheng , Zhang Xin , Wang Junjie , Liu Yun , You Yongping , Yu Yun 
Published: August 2023

ObjectivesIn adult diffuse glioma, preoperative detection of isocitrate dehydrogenase (IDH) status helps clinicians develop surgical strategies and evaluate patient prognosis. Here, we aim to identify an optimal machine-learning model for prediction of IDH genotyping by combining deep-learning (DL) signatures and conventional radiomics (CR) features as model predictors.MethodsIn this study, a total of 486 patients with adult diffuse gliomas were retrospectively collected from our medical center (n=268) and the public database (TCGA, n=218). All included patients were randomly divided into the training and validation sets by using nested 10-fold cross-validation. A total of 6,736 CR features were extracted from four MRI modalities in each patient, namely T1WI, T1CE, T2WI, and FLAIR. The LASSO algorithm was performed for CR feature selection. In each MRI modality, we applied a CNN+LSTM–based neural network to extract DL features and integrate these features into a DL signature after the fully connected layer with sigmoid activation. Eight classic machine-learning models were analyzed and compared in terms of their prediction performance and stability in IDH genotyping by combining the LASSO–selected CR features and integrated DL signatures as model predictors. In the validation sets, the prediction performance was evaluated by using accuracy and the area under the curve (AUC) of the receiver operating characteristics, while the model stability was analyzed by using the relative standard deviation of the AUC (RSDAUC). Subgroup analyses of DL signatures and CR features were also individually conducted to explore their independent prediction values.ResultsLogistic regression (LR) achieved favorable prediction performance (AUC: 0.920 ± 0.043, accuracy: 0.843 ± 0.044), whereas support vector machine with the linear kernel (l-SVM) displayed low prediction performance (AUC: 0.812 ± 0.052, accuracy: 0.821 ± 0.050). With regard to stability, LR also showed high robustness against data perturbation (RSDAUC: 4.7%). Subgroup analyses showed that DL signatures outperformed CR features (DL, AUC: 0.915 ± 0.054, accuracy: 0.835 ± 0.061, RSDAUC: 5.9%; CR, AUC: 0.830 ± 0.066, accuracy: 0.771 ± 0.051, RSDAUC: 8.0%), while DL and DL+CR achieved similar prediction results.ConclusionIn IDH genotyping, LR is a promising machine-learning classification model. Compared with CR features, DL signatures exhibit markedly superior prediction values and discriminative capability.
Phase Ib trial of inhaled iloprost for the prevention of lung cancer with predictive and response biomarker assessment
Authors: Miller York E. , Ghosh Moumita , Merrick Daniel T. , Kubala Brandi , Szabo Eva , Bengtson Lisa , Kocherginsky Masha , Helenowski Irene B. , Benante Kelly , Schering Tia , Kim Jihye , Kim Hyunmin , Ha Duc , Bergan Raymond C. , Khan Seema A. , Keith Robert L. 
Published: August 2023

IntroductionIloprost, a prostacyclin analog, has lung cancerpreventive activity in preclinical models and improved dysplasia in former smokers in a phase IIb trial. Oral iloprost is currently unavailable. We performed a phase Ib trial of inhaled iloprost in former smokers to assess tolerance and compliance.MethodsParticipants self-administered nebulized iloprost (5ug) or placebo four (QID) or two (BID) times daily. As QID dose was well tolerated and due to expiration of the placebo, the BID dosing and placebo were eliminated early on in the trial. Bronchoscopy with biopsyat six standard sites was performed at treatment initiation and two months post-iloprost, with exploratory histological analysis. Bulk RNA sequencing, single cell RNA sequencing and an in vitro assay of epithelial progenitor cell iloprost response were performed on a subset of biopsies in an exploratory investigation of response mechanisms and predictive biomarkers.Results and discussionThirty-four of a planned 48 participants were recruited to the trial.Inhaled iloprost was well tolerated with no adverse events &gt; grade 2. Compliance was 67% in the QID group. The trial was not powered to detect histologic response and none was found. Bulk RNA sequencing of biopsies pre/post iloprost suggest that iloprost is immunomodulatory and downregulates cell proliferation pathways. Single cell RNA sequencing showed an increase in CD8-positive T cells with upregulation of genes in interferon γ signaling. In vitro iloprost response by epithelial progenitor cells correlated with histologic response with kappa coefficient of 0.81 (95% CI 0.47, 1.0). Inhaled iloprost was well tolerated with suboptimal compliance. Molecular analysis suggested that iloprosthas immunomodulatory and antiproliferative effects.The progenitor cell iloprost response assay may be a promising avenue to develop predictive biomarkers.Clinical trial registrationhttps://clinicaltrials.gov/study/NCT02237183, identifier NCT02237183.
Artesunate, as an Hsp90 inhibitor, inhibits the proliferation of Burkitt’s lymphoma cells by inhibiting AKT and ERK
Authors: Yuan-Ce Li , Qi Zhang , Hong-Yang Zhang , Yan-Wen Wang , Yu-Mei Sun , Bi-Juan Yang , Jun-Lin Yin 
Published: August 2023

Introduction: Artesunate, a derivative of artemisinin, has anti-malarial effects, and in recent years has also been reported to have anti-tumor activity. However, its anti-tumor mechanisms are not well understood.Methods: In this study, we focused on the targeting of Hsp90 by artesunate to inhibit tumor cell proliferation, which we examined using immunoprecipitation, a proliferation assay, flow cytometry, western blotting, a tumor xenograft animal model, and immunohistochemistry. Furthermore, to examine the tumor-suppressive effects of artesunatein nude mice, we used artesunate-loaded PLGA-PEG nanoparticles.Results: The binding of artesunate to Hsp90 was found to reduce the expression of its client proteins AKT, ERK, p-AKT, p-ERK, and EGFR, thereby blocking the cell cycle at the G0/G1 → S stage in lymphoma cells and inducing apoptosis. In addition, the results of tumor xenograft experiments revealed that artesunate reduced the expression of AKT and ERK proteins in tumor tissues, inhibited tumor proliferation, and reduced tumor size and weight. Furthermore, nanoparticle encapsulation was demonstrated to enhance the anti-cancer activity of artesunate.Discussion: We thus established that artesunate inhibits the proliferation of lymphoma cells by targeting the Hsp90 protein, and we accordingly believe that this compound has potential for development as a novelanti-tumor drug.