bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sarcomatoid carcinoma of the gallbladder: A rare and aggressive form of gallbladder cancer
Authors: Limaiem Faten , Bayar Rached , Omrani Sahir 
Published: January 2023

AbstractSarcomatoid carcinoma is a rare type of gallbladder cancer with less than 100 cases reported in the literature. Preoperative diagnosis is difficult due to nonspecific clinical and radiological findings. Its prognosis is poor due to rapid progression and a high short‐term recurrence rate. The definitive diagnosis relies on histopathological examination.
Laparoscopic resection of a uterine lipoleiomyoma
Authors: Limaiem Faten , Halouani Ahmed , Ben Aissia Nizar 
Published: January 2023

AbstractUterine lipoleiomyomas are rare variants of leiomyomas that usually occur in postmenopausal women. Their incidence ranges from 0.03% to 0.2%. Few cases of laparoscopically resected lipoleiomyomas have been reported in the literature. Further accumulation of lipoleiomyomas could help establish an accurate preoperative diagnosis of this uncommon and benign mesenchymal tumor.
Synthesis of Open‐Cage Fullerene Derivatives Containing Multiple Hydroxyl and Amino Groups on the Rim of the Orifice†
Authors: Liu Xueli , Gao Rui , Liu Zhen , Ming Jialin , Qiu Yi , Su Jie , Gan Liangbing 
Published: January 2023

Comprehensive SummaryMost of the known open‐cage fullerene derivatives contain carbonyl and other relatively inert groups on the rim of the orifice. It is difficult to rationally design further reactions and attach other functional groups on to these open‐cage derivatives. In the present work, two molecules of difunctional 1,4‐benzenediamine have been incorporated into the rim of an open‐cage C60 derivative through one amino group leaving the other amino group free for further functionalization. The difunctional 4‐aminophenol reacted analogously to form an open‐cage derivative with a free OH group each on the two phenyl rings. The amino and hydroxyl groups on the phenyl ring above the rim of the orifice showed similar reactivity as aniline and phenol. One of the carbonyl groups on the rim of the orifice could be selectively reduced by NaBH4 and P(OEt)3. The reduction reactions were reversible and the reduced products could be readily converted back to the carbonyl precursor. Thus, this redox process acts as a tool to fine tune the size of the orifice for host‐guest studies.
Testing for Facioscapulohumeral Muscular Dystrophy with Optical Genome Mapping
Authors: Koppikar Pratik , Shenoy Suresh , Guruju Naga , Hegde Madhuri 
Published: January 2023

AbstractThe introduction of optical genome mapping has improved time constraints and a lack of specificity from previous methodologies when performing genome‐wide analyses of samples. Optical genome mapping allows for the detection of structural variations, aberrations, and functionality traits from a single stained molecule of DNA. Though the preparation time is increased compared to previously utilized visualization techniques, optical genome mapping significantly reduces the time needed for analysis. Specifically, individual disease pipelines have been developed to rapidly analyze prepared samples. One of these diseases, Facioscapulohumeral Muscular Dystrophy (FSHD), is detected through quantification of the D4Z4 repeat array on chromosome 4q35. Optical genome mapping, with the ability to enumerate the repeats of the D4Z4 array, has demonstrated the capability to precisely diagnose FSHD. In this protocol, the preparation of samples and subsequent loading and analysis in an optical genome mapping system is discussed for the detection and analysis of FSHD. These methods should prove highly useful in FSHD analyses and beyond with the development of further disease analysis pipelines within the instrument. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Genomic DNA isolation, labeling, and stainingBasic Protocol 2: Mapping and analysis with the Bionano Saphyr® system
Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer
Authors: Sivakumar Suresh , Lieber Sonja , Librizzi Damiano , Keber Corinna , Sommerfeld Leah , Finkernagel Florian , Roth Katrin , Reinartz Silke , Bartsch Jörg W. , Graumann Johannes , Müller‐Brüsselbach Sabine , Müller Rolf 
Published: January 2023

AbstractBackgroundBasal cell adhesion molecule (BCAM) is a laminin α5 (LAMA5) binding membrane‐bound protein with a putative role in cancer. Besides full‐length BCAM1, an isoform lacking most of the cytoplasmic domain (BCAM2), and a soluble form (sBCAM) of unknown function are known. In ovarian carcinoma (OC), all BCAM forms are abundant and associated with poor survival, yet BCAM's contribution to peritoneal metastatic spread remains enigmatic.MethodsBiochemical, omics‐based and real‐time cell assays were employed to identify the source of sBCAM and metastasis‐related functions of different BCAM forms. OC cells, explanted omentum and a mouse model of peritoneal colonisation were used in loss‐ and gain‐of‐function experiments.ResultsWe identified ADAM10 as a major BCAM sheddase produced by OC cells and identified proteolytic cleavage sites proximal to the transmembrane domain. Recombinant soluble BCAM inhibited single‐cell adhesion and migration identically to membrane‐bound isoforms, confirming its biological activity in OC. Intriguingly, this seemingly anti‐tumorigenic potential of BCAM contrasts with a novel pro‐metastatic function discovered in the present study. Thus, all queried BCAM forms decreased the compactness of tumour cell spheroids by inhibiting LAMA5 – integrin β1 interactions, promoted spheroid dispersion in a three‐dimensional collagen matrix, induced clearance of mesothelial cells at spheroid attachment sites in vitro and enhanced invasion of spheroids into omental tissue both ex vivo and in vivo.ConclusionsMembrane‐bound BCAM as well as sBCAM shed by ADAM10 act as decoys rather than signalling receptors to modulate metastasis‐related functions. While BCAM appears to have tumour‐suppressive effects on single cells, it promotes the dispersion of OC cell spheroids by regulating LAMA5‐integrin‐β1‐dependent compaction and thereby facilitating invasion of metastatic target sites. As peritoneal dissemination is majorly mediated by spheroids, these findings offer an explanation for the association of BCAM with a poor clinical outcome of OC, suggesting novel therapeutic options.
Rapid screening of 2‐benzylbenzimidazole nitazene analogs in suspect counterfeit tablets using Raman, SERS, DART‐TD‐MS, and FT‐IR
Authors: Kimani Martin M. , Kern Sara , Lanzarotta Adam , Thatcher Michael , Lorenz Lisa M. , Smith Skyler W. , Collins Melissa , Howe Gregory W. , Wetherby Anthony E. 
Published: January 2023

AbstractDeveloping methods to rapidly screen for novel synthetic 2‐benzylbenzimidazole opioids, also known as nitazenes, has become increasingly important due to their high potency. These compounds have potency comparable or exceeding that of fentanyl by up to 10 times and have been implicated in approximately 5% of all drug overdose deaths in the United States in 2021. This paper details the authenticity determination of suspect tablets and the identification of three nitazene analogs (N‐pyrrolidino etonitazene, isotonitazene, and etodesnitazene) in suspect tablets seized at a mail facility using Raman and surface‐enhanced Raman scattering (SERS) with handheld devices, portable Fourier transform infrared spectrometer (FT‐IR), and a direct analysis in real‐time ambient ionization coupled to a thermal desorption unit and a mass spectrometer (DART‐TD‐MS). These methods are rapid and excellent for screening opioids in suspect tablets but could not fully determine the exact structure of some of the nitazene analogs present due to spectral similarities or similar fragmentation patterns. Liquid chromatography–mass spectrometry (LC–MS) confirmed the presence of these nitazene compounds in addition to other opioids/drugs that were in trace quantities. The quantitative high‐performance liquid chromatography coupled with ultraviolet (HPLC‐UV) detection experiments determined that the suspect tablets contained an average of 0.817 mg of N‐pyrrolidino etonitazene per tablet. The results obtained reveal that the simultaneous deployment of these complementary and orthogonal portable analytical techniques as part of a workflow allows suspect tablets to be screened and nitazene‐type drugs to be identified in suspect counterfeit tablets at remote sampling sites.
Identification of runs of homozygosity in Western honey bees (Apis mellifera) using whole‐genome sequencing data
Authors: Gmel Annik Imogen , Guichard Matthieu , Dainat Benjamin , Williams Geoffrey Rhys , Eynard Sonia , Vignal Alain , Servin Bertrand ,   the Beestrong Consortium, Neuditschko Markus 
Published: January 2023

AbstractRuns of homozygosity (ROH) are continuous homozygous segments that arise through the transmission of haplotypes that are identical by descent. The length and distribution of ROH segments provide insights into the genetic diversity of populations and can be associated with selection signatures. Here, we analyzed reconstructed whole‐genome queen genotypes, from a pool‐seq data experiment including 265 Western honeybee colonies fromApis mellifera melliferaandApis mellifera carnica. Integrating individual ROH patterns and admixture levels in a dynamic population network visualization allowed us to ascertain major differences between the two subspecies. WithinA. m. mellifera, we identified well‐defined substructures according to the genetic origin of the queens. Despite the current applied conservation efforts, we pinpointed 79 admixed queens. Genomic inbreeding (FROH) strongly varied within and between the identified subpopulations. ConservedA. m. melliferafrom Switzerland had the highest meanFROH(3.39%), while queens originating from a conservation area in France, which were also highly admixed, showed significantly lowerFROH(0.45%). The majority ofA. m. carnicaqueens were also highly admixed, except 12 purebred queens with a meanFROHof 2.33%. Within the breed‐specific ROH islands, we identified 14 coding genes forA. m. melliferaand five forA. m. carnica, respectively. Local adaption ofA. m. melliferacould be suggested by the identification of genes involved in the response to ultraviolet light (Crh‐BP,Uvop) and body size (Hex70a,Hex70b), while theA. m. carnicaspecific genesCpr3andCpr4are most likely associated with the lighter striping pattern, a morphological phenotype expected in this subspecies. We demonstrated that queen genotypes derived from pooled workers are useful tool to unravel the population dynamics inA. melliferaand provide fundamental information to conserve native honey bees.
Nested singletons in molecular trees: Utility of adding morphological and geographical data from digitized herbarium specimens to test taxon concepts at species level in the case of Casearia (Salicaceae)
Authors: de Mestier Astrid , Lücking Robert , Gutierrez Jorge , Brokamp Grischa , Celis Marcela , Borsch Thomas 
Published: January 2023

AbstractUsing the genus Casearia, we assessed the status of nested singletons: individual specimens corresponding to accepted species but in molecular trees appearing nested within clades of closely related species. Normally, such cases would be left undecided, while on the other hand, timely taxonomic decisions are required. We argue that morphological, chorological, and ecological data can be informative to illuminate patterns of speciation. Their use can provide a first step in testing taxon concepts at species level. We focused on five cases of nested singletons in trees of the genus Casearia. We employed PCA and cluster analysis to assess phenotypic differentiation. Using geocoordinates, we calculated niche space differentiation based on 19 bioclim variables, by means of PCA and niche equivalency and similarity tests and generated dot maps. We found that the singletons were morphologically distinctive in two of the five cases (Casearia selloana and C. manausensis), relatively distinctive in two other cases (C. zizyphoides and C. mariquitensis), and partially overlapping in the last case (C. grandiflora). For two cases (C. mariquitensis and C. selloana), ecological niche space was broadly overlapping, in two cases it was found broadly nested (C. grandiflora and C. zizyphoides), and in one case narrowly nested (C. manausensis), but in no case niche differentiation was observed. Niche overlap, similarity and equivalency showed corresponding patterns. Given these data, one would interpret C. selloana and C. manausensis as presumably well‐distinguished taxa, their narrow distribution ranges suggesting recently emerging lineages. The other three cases are not clearcut. Morphological data would suggest particularly C. grandiflora conspecific with C. arborea, but differences in the distribution are intriguing. Our approach would reject the notion of potential synonymy based on nested phylogenetic placement for at least two of the five cases. The other case also shows no complete lack of differentiation which would support synonymy.
Iron therapy and severe arrhythmias in HFrEF: rationale, study design, and baseline results of the RESAFE‐HF trial
Authors: Bakogiannis Constantinos , Mouselimis Dimitrios , Tsarouchas Anastasios , Papadopoulos Christodoulos E. , Theofillogiannakos Efstratios K. , Lechat Elmira , Antoniadis Antonios P. , Pagourelias Efstathios D. , Kelemanis Ioannis , Tzikas Stergios , Fragakis Nikolaos , Efthimiadis Georgios K. , Karamitsos Theodoros D. , Doumas Michael , Vassilikos Vassilios P. 
Published: January 2023

AbstractAimsThe Iron Intravenous Therapy in Reducing the burden of Severe Arrhythmias in HFrEF (RESAFE‐HF) registry study aims to provide real‐word evidence on the impact of intravenous ferric carboxymaltose (FCM) on the arrhythmic burden of patients with heart failure with reduced ejection fraction (HFrEF), iron deficiency (ID), and implanted cardiac implantable electronic devices (CIEDs).Methods and resultsThe RESAFE‐HF (NCT04974021) study was designed as a prospective, single‐centre, and open‐label registry study with baseline, 3, 6, and 12 month visits. Adult patients with HFrEF and CIEDs scheduled to receive IV FCM as treatment for ID as part of clinical practice were eligible to participate. The primary endpoint is the composite iron‐related endpoint of haemoglobin ≥ 12 g/dL, ferritin ≥ 50 ng/L, and transferrin saturation > 20%. Secondary endpoints include unplanned HF‐related hospitalizations, ventricular tachyarrhythmias detected by CIEDs and Holter monitors, echocardiographic markers, functional status (VO2 max and 6 min walk test), blood biomarkers, and quality of life. In total, 106 patients with a median age of 72 years (14.4) were included. The majority were male (84.9%), whereas 92.5% of patients were categorized to New York Heart Association II/III. Patients' arrhythmic burden prior to FCM administration was significant—19 patients (17.9%) received appropriate CIED therapy for termination of ventricular tachyarrhythmia in the preceding 12 months, and 75.5% of patients have frequent, repetitive multiform premature ventricular contractions.ConclusionsThe RESAFE‐HF trial is expected to provide evidence on the effect of treating ID with FCM in HFrEF based on real‐world data. Special focus will be given on the arrhythmic burden post‐FCM administration.
Recent Advances in Surface Coatings of Layered Cathode Materials for High‐Performance Sodium‐Ion Batteries
Authors: Zhou Yan , Li Laishi , Wu Yusheng , Xie Hongwei 
Published: January 2023

AbstractLayered transition metal oxides (layered materials) have the advantages of simple synthesis methods, high average operating voltages, and good specific capacity, and are therefore promising cathode materials for sodium‐ion batteries (SIBs). However, the capacity retention of these materials is poor due to the dissolution of transition metals caused by the detrimental reactions of the electrode with the electrolyte and the rupture of the electrode due to volume expansion during cycling. Studies have discovered that surface modification can effectively improve the aforementioned problems. This paper reviews the effects of different coating materials (e. g., carbon coatings, metal oxide coatings, phosphate coatings, etc.) on the performances of layered cathode materials and analyzes the reasons for the improved performance. In addition, the limitations of different coating materials and coating methods are presented, and future developments are proposed.
Cardiac structural and functional abnormalities in epilepsy: A systematic review and meta‐analysis
Authors: Liu Zining , Thergarajan Peravina , Antonic‐Baker Ana , Chen Zhibin , Sparks Paul B. , Lannin Natasha A. , Kwan Patrick , Jones Nigel C. , Casillas‐Espinosa Pablo M. , Perucca Piero , O'Brien Terence J. , Sivathamboo Shobi 
Published: January 2023

AbstractObjectiveEpilepsy is associated with an increased risk of cardiovascular disease and mortality. Whether cardiac structure and function are altered in epilepsy remains unclear. To address this, we conducted a systematic review and meta‐analysis of studies evaluating cardiac structure and function in patients with epilepsy.MethodsWe searched the electronic databases MEDLINE, PubMed, COCHRANE, and Web of Science from inception to 31 December 2021. Primary outcomes of interest included left ventricular ejection fraction (LVEF) for studies reporting echocardiogram findings and cardiac weight and fibrosis for postmortem investigations. Study quality was assessed using the National Heart, Lung, and Blood Institute (NHLBI) assessment tools.ResultsAmong the 10 case‐control studies with epilepsy patients (n = 515) and healthy controls (n = 445), LVEF was significantly decreased in epilepsy group compared with controls (MD: −1.80; 95% confidence interval [CI]: −3.56 to −0.04; P = 0.045), whereas A‐wave velocity (MD: 4.73; 95% CI: 1.87‐7.60; P = 0.001), E/e' ratio (MD: 0.39; 95% CI: 0.06‐0.71; P = 0.019), and isovolumic relaxation time (MD: 10.18; 95% CI: 2.05‐18.32; P = 0.014) were increased in epilepsy, compared with controls. A pooled analysis was performed in sudden unexpected death in epilepsy (SUDEP) cases with autopsy data (n = 714). Among SUDEP cases, the prevalence of cardiac hypertrophy was 16% (95% CI: 9%–23%); cardiac fibrosis was 20% (95% CI: 15%–26%). We found no marked differences in cardiac hypertrophy, heart weight, or cardiac fibrosis between SUDEP cases and epilepsy controls.SignificanceOur findings suggest that epilepsy is associated with altered diastolic and systolic echocardiogram parameters compared with healthy controls. Notably, SUDEP does not appear to be associated with a higher incidence of structural cardiac abnormalities, compared with non‐SUDEP epilepsy controls. Longitudinal studies are needed to understand the prognostic significance of such changes. Echocardiography may be a useful noninvasive diagnostic test in epilepsy population.
Optimal and sub‐optimal rate and power allocation for random networks
Authors: Nativ Zucker Sapir , Bergel Itsik 
Published: January 2023

AbstractWe consider the power and rate allocation for a transmission in a wireless network where the transmitter knows the channel state of the desired receiver but only knows the statistics of the interferes. While this scenario is often encountered in practice, and extremely important in dense networks, its optimal solution was not yet known. The analysis considers nodes that are distributed according to a homogeneous Poisson point process, and hence applies both to cellular and to ad‐hoc networks. We derive (for the first time) the optimal power allocation at a node, based only on the channel gain to its desired receiver. We also derive the optimal rate allocation in networks that are characterized by the outage rate model. Considering sub‐optimal allocations, we advocate the use of the simple threshold scheduling scheme for power allocation and linear rate adaptation for rate allocation. We prove that these suboptimal schemes are close to optimal, for any channel fading. We also present simulation results over Rayleigh fading channels that show a maximum loss of 1.5% between the advocated schemes and the optimal schemes.
From financial wealth shocks to ill‐health: Allostatic load and overload
Authors: French Declan 
Published: January 2023

AbstractA number of studies have associated financial wealth changes with health‐related outcomes arguing that the effect is due to psychological distress and is immediate. In this paper, I examine this relationship for cumulative shocks to the financial wealth of American retirees using the allostatic load model of pathways from stress to poor health. Wealth shocks are identified from Health and Retirement Study reports of stock ownership along with significant negative discontinuities in high‐frequency S&P500 index data. I find that a one standard deviation increase in cumulative shocks over two years increases the probability of elevated blood pressure by 9.5%, increases waist circumference by 1.2% and the cholesterol ratio by 6.1% for those whose wealth is all in shares. My findings suggest that the combined effect of random shocks to financial wealth over time is salient for health outcomes. This is consistent with the allostatic load model in which repeated activation of stress responses leads to cumulative wear and tear on the body.
N‐Propargylic β‐enaminones in breast cancer cells: Cytotoxicity, apoptosis, and cell cycle analyses
Authors: Ilhan Suleyman , Atmaca Harika , Yilmaz Elif Serel , Korkmaz Esra , Zora Metin 
Published: January 2023

AbstractBreast cancer is one of the most common cancers worldwide and the discovery of new cytotoxic agents is needed. Enaminones are regarded to be a significant structural motif that is found in a variety of pharmacologically active compounds however the number of studies investigating the anticancer activities of N‐propargylic β‐enaminones (NPEs) is limited. Herein we investigated the potential cytotoxic and apoptotic effects of 23 different NPEs (1‐23) on human breast cancer cells. Cytotoxicity was evaluated via MTT assay. Apoptotic cell death and cell cycle distributions were investigated by flow cytometry. CM‐H2DCFDA dye was used to evaluate cellular ROS levels. Expression levels of Bcl‐2, Bax, p21, and Cyclin D1 were measured by quantitative real‐time PCR. ADME properties were calculated using the ADMET 2.0 tool. NPEs 4, 9, 16, and 21 showed selective cytotoxic activity against breast cancer cells with SI values >2. NPEs induced apoptosis and caused significant changes in Bcl‐2 and Bax mRNA levels. The cell cycle was arrested at the G0/G1 phase and levels of p21 and Cyclin D1 were upregulated in both breast cancer cells. ROS levels were significantly increased by NPEs, suggesting that the cytotoxic and apoptotic effects of NPEs were mediated by ROS. ADME analysis revealed that NPEs showed favorable distributions in both breast cancer cell lines, meaning good lipophilicity values, low unfractionated values, and high bioavailability. Therefore, these potential anticancer compounds should be further validated by in vivo studies for their appropriate function in human health with a safety profile, and a comprehensive drug interaction study should be performed.