bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Bridge Exercise Duration on Lateral Abdominal Muscle Thickness and Gluteus Maximus Activation
Authors: Kellis Eleftherios , Konstantopoulos Athanasios , Ellinoudis Athanasios 
Published: May 2023

Context: Bridge exercises are extensively used in trunk-strengthening programs. The aim of this study was to investigate the effect of bridging duration on lateral abdominal muscle thickness and gluteus maximus activation. Design: Cross-sectional. Methods: Twenty-five young males participated in this study. Transversus abdominal (TrA), external and internal oblique ultrasound thickness, gluteus maximus electromyographic activation, and sacral tilt angle were simultaneously measured for every second during 30-second bridging exercise. The contraction thickness ratio and root mean squared signal (normalized to maximum isometric contraction signal) during 6 exercise durations (from 0 to 5, 10, 15, 20, 25, and 30 s) were also calculated and compared using analysis of variance designs. Results: TrA and internal oblique contraction thickness ratio and gluteus maximus root mean squared increased during the first 8 to 10 seconds and remained elevated until the end of the 30-second exercise (P < .05). External oblique contraction thickness ratio declined during exercise (P < .05). Five-second bridging showed less TrA thickness and anteroposterior and mediolateral sacral tilt angle and a lower anteroposterior tilt variability compared with bridges, which lasted more than 10 seconds (P < .05). Conclusions: Bridge exercises longer than 10 seconds may be better for promoting TrA recruitment than bridges of shorter duration. Clinicians and exercise specialists could adjust the duration of bridge exercise based on the aims of the exercise program.
Inhibition of miR-200b-3p confers broad-spectrum resistance to viral infection by targeting TBK1
Authors: Fang An , Yuan Yueming , Sui Baokuen , Wang Zhihui , Zhang Yuan , Zhou Ming , Chen Huanchun , Fu Zhen F. , Zhao Ling 
Published: May 2023

ABSTRACT
The host innate immune system’s defense against viral infections depends heavily on type I interferon (IFN-I) production. Research into the mechanisms of virus-host interactions is essential for developing novel antiviral therapies. In this study, we compared the effect of the five members of the microRNA-200 (miR-200) family on IFN-I production during viral infection and found that miR-200b-3p displayed the most pronounced regulatory effect. During viral infection, we discovered that the transcriptional level of microRNA-200b-3p (miR-200b-3p) increased with the infection of influenza virus (IAV) and vesicular stomatitis virus (VSV), and miR-200b-3p production was modulated by the activation of the ERK and p38 pathways. We identified cAMP response element binding protein (CREB) as a novel transcription factor that binds to the miR-200b-3p promoter. MiR-200b-3p reduces NF-κB and IRF3-mediated IFN-I production by targeting the 3′ untranslated region (3′ UTR) of TBK1 mRNA. Applying miR-200b-3p inhibitor enhances IFN-I production in IAV and VSV-infected mouse models, thus inhibiting viral replication and improving mouse survival ratio. Importantly, in addition to IAV and VSV, miR-200b-3p inhibitors exhibited potent antiviral effects against multiple pathogenic viruses threatening human health worldwide. Overall, our study suggests that miR-200b-3p might be a potential therapeutic target for broad-spectrum antiviral therapy.

IMPORTANCE
The innate immune response mediated by type I interferon (IFN-I) is essential for controlling viral replication. MicroRNAs (miRNAs) have been found to regulate the IFN signaling pathway. In this study, we describe a novel function of miRNA-200b-3p in negatively regulating IFN-I production during viral infection. miRNA-200b-3p was upregulated by the MAPK pathway activated by IAV and VSV infection. The binding of miRNA-200b-3p to the 3′ UTR of TBK1 mRNA reduced IFN-I activation mediated by IRF3 and NF-κB. Application of miR-200b-3p inhibitors exhibited potent antiviral effects against multiple RNA and DNA viruses. These results provide fresh insight into understanding the impact of miRNAs on host-virus interactions and reveal a potential therapeutic target for common antiviral intervention.
Flotillin-associated rhodopsin (FArhodopsin), a widespread paralog of proteorhodopsin in aquatic bacteria with streamlined genomes
Authors: Haro-Moreno Jose M. , López-Pérez Mario , Alekseev Alexey , Podoliak Elizaveta , Kovalev Kirill , Gordeliy Valentin , Stepanauskas Ramunas , Rodriguez-Valera Francisco 
Published: May 2023

ABSTRACT

Microbial rhodopsins are found more than once in a single genome (paralogs) often have different functions. We screened a large dataset of open ocean single-amplified genomes (SAGs) for co-occurrences of multiple rhodopsin genes. Many such cases were found among Pelagibacterales (SAR11), HIMB59, and the Gammaproteobacteria
Pseudothioglobus
SAGs. These genomes always had a
bona fide
proteorhodopsin and a separate cluster of genes containing a second rhodopsin associated with a predicted flotillin coding gene and have thus been named flotillin-associated rhodopsins (FArhodopsins). Although they are members of the proteorhodopsin protein family, they form a separate clade within that family and are quite divergent from known proton-pumping proteorhodopsins. They contain either DTT, DTL, or DNI motifs in their key functional amino acids. FArhodopsins are mainly associated with the lower layers of the epipelagic zone. All marine FArhodopsins had the retinal binding lysine, but we found relatives in freshwater metagenomes lacking this key amino acid. AlphaFold predictions of marine FArhodopsins indicate that their retinal pocket might be very reduced or absent, hinting that they are retinal-less. Freshwater FArhodopsins were more diverse than marine ones, but we could not determine if there were other rhodopsins in the genome due to the lack of SAGs or isolates. Although the function of FArhodopsins could not be established, their conserved genomic context indicated involvement in the formation of membrane microdomains. The conservation of FArhodopsins in diverse and globally abundant microorganisms suggests that they may be important in the adaptation to the twilight zone of aquatic environments.

IMPORTANCE
Rhodopsins have been shown to play a key role in the ecology of aquatic microbes. Here, we describe a group of widespread rhodopsins in aquatic microbes associated with dim light conditions. Their characteristic genomic context found in both marine and freshwater environments indicates a novel potential involvement in membrane microstructure that could be important for the function of the coexisting proteorhodopsin proton pumps. The absence or reduction of the retinal binding pocket points to a drastically different physiological role.
Testing the applicability and additional value of a consultation round after the consensus meeting in the development of two core outcome sets
Authors: Beune Irene Maria , Jansen Larissa , Grooten Iris , Ganzevoort Wessel , Painter Rebecca C , Gordijn Sanne 
Published: May 2023

ObjectivesTest applicability and additional value of a consultation round after the consensus meeting in the development of core outcome sets (COSs).Study design and settingIn two COS procedures (Core Outcome Set for the prevention and treatment of fetal GROwth restriction: deVeloping Endpoints (COSGROVE) and Definition and Core Outcomes on Hyperemesis Gravida (DCOHG)) that followed the Core Outcome Measures in Effectiveness Trials methodology, the first round of convergence to consensus among stakeholder groups in an online Delphi procedure was followed by a face-to-face consensus meeting during which a COS was formulated. We subsequently presented the COS to the online panel in a consultation round to confirm that the online panel agreed with the choices made at the consensus meeting, defined as 80% agreement.ParticipantsIn the COSGROVE Study, there were eight stakeholder groups, and 83 out of 107 participants completed the consultation round. In the DCOHG Study, there were four stakeholder groups, and 96 out of 125 completed the consultation round.InterventionsAdding a consultation round after completing a modified Delphi method with a consensus meeting.ResultsThere was a level of agreement of 81% and 84%, respectively, in the consultation round of both procedures. This was above the preset level of agreement. The consultation round yielded additional suggestions to refine COS formulation in one of the studies.ConclusionOur study shows that in two procedures, the online expert panel agreed with the participants of the consensus meeting in these procedures, lending validity to existing COS methodology. Future studies could evaluate whether bringing back the COS for confirmation after the consensus meeting could potentially increase the uptake of the final COS.
Time trends in the incidence of cardiovascular disease, hypertension and diabetes by sex and socioeconomic status in Catalonia, Spain: a population-based cohort study
Authors: Bennett Matthew , Pistillo Andrea , Recalde Martina , Reyes Carlen , Freisling Heinz , Duarte-Salles Talita 
Published: May 2023

ObjectiveWe aimed to estimate how longitudinal trends in cardiovascular disease, hypertension and type 2 diabetes mellitus incidence in Catalonia, Spain from 2009 to 2018 may differ by age, sex and socioeconomic deprivation.DesignCohort study using prospectively collected data.SettingElectronic health records from primary healthcare centres in Catalonia, Spain.Participants3 247 244 adults (≥40 years).Outcome measuresWe calculated the annual incidence (per 1000 persons-year) and incidence rate ratios (IRR) between three time periods of cardiovascular disease, hypertension and type 2 diabetes mellitus to measure trends and changes in incidence during the study period.ResultsIn 2016–2018 compared with 2009–2012, cardiovascular disease incidence increased in the 40–54 (eg, IRR=1.61, 95% CI: 1.52 to 1.69 in women) and 55–69 age groups. There was no change in cardiovascular disease incidence in women aged 70+ years, and a slight decrease in men aged 70+ years (0.93, 0.90 to 0.95). Hypertension incidence decreased in all age groups for both sexes. Type 2 diabetes mellitus incidence decreased in all age groups for both sexes (eg, 0.72, 0.70 to 0.73 in women aged 55–69 years), except for the 40–54 year age group (eg, 1.09, 1.06 to 1.13 in women). Higher incidence levels were found in the most deprived areas, especially in the 40–54 and 55–69 groups.ConclusionsOverall cardiovascular disease incidence has increased while hypertension and type 2 diabetes mellitus incidence have decreased in the last years in Catalonia, Spain, with differences in trends by age group and socioeconomic deprivation.
Fostering practice-based evidence through routine outcome monitoring in a university psychotherapy service for common mental health problems: a protocol for a naturalistic, observational study
Authors: Valdiviezo-Oña Jorge , Montesano Adrián , Evans Chris , Paz Clara 
Published: May 2023

IntroductionData-informed psychotherapy and routine outcome monitoring are growing as referents in psychotherapy research and practice. In Ecuador, standardised web-based routine outcome monitoring systems have not been used yet, precluding data-driven clinical decisions and service management. Hence, this project aims at fostering and disseminating practice-based evidence in psychotherapy in Ecuador by implementing a web-based routine outcome monitoring system in a university psychotherapy service.Methods and analysesThis is a protocol for an observational naturalistic longitudinal study. Progress and outcomes of treatment in the Centro de Psicología Aplicada of the Universidad de Las Américas in Quito, Ecuador will be examined. Participants will be adolescents and adults (≥11 years) seeking treatment, as well as therapists and trainees working at the centre between October 2022 and September 2025. Clients’ progress will be monitored by a range of key variables: psychological distress, ambivalence to change, family functioning, therapeutic alliance and life satisfaction. Sociodemographic information and satisfaction with treatment data will be collected before and at the end of treatment, respectively. Also, semi-structured interviews to explore therapists’ and trainees’ perceptions, expectations and experiences will be conducted. We will analyse first contact data, psychometrics of the measures, reliable and clinically significant change, outcome predictors as well as trajectories of changes. Moreover, we will conduct a framework analysis for the interviews.Ethics and disseminationThe protocol for this study was approved by the Human Research Ethics Committee of the Pontificia Universidad Católica del Ecuador (#PV-10–2022). The results will be disseminated in peer-reviewed scientific articles, at conferences and in workshops.Trial registration numberNCT05343741.
Green Cross method in a postanaesthesia care unit: a qualitative study of the healthcare professionals’ experiences after 3 years, including the COVID-19 pandemic period
Authors: Birkeli Gørill Helen , Ballangrud Randi , Jacobsen Hilde Kristin , Tveter Deilkas Ellen Catharina , Lindahl Anne Karin 
Published: May 2023

ObjectivesUnsafe medical care causes morbidity and mortality among the hospital patients. In a postanaesthesia care unit (PACU), increasing patient safety is a joint effort between different professions. The Green Cross (GC) method is a user-friendly incident reporting method that incorporates daily safety briefings to support healthcare professionals in their daily patient safety work. Thus, this study aimed to describe healthcare professionals’ experiences with the GC method in a PACU setting 3 years after its implementation, including the period of the coronavirus disease 2019 pandemic’s three waves.DesignAn inductive, descriptive qualitative study was conducted. The data were analysed using qualitative content analysis.SettingThe study was conducted at a PACU of a university hospital in South-Eastern Norway.ParticipantsFive semistructured focus group interviews were conducted in March and April 2022. The informants (n=23) were PACU nurses (n=18) and collaborative healthcare professionals (n=5) including physicians, nurses and a pharmacist.ResultsThe theme ‘still active, but in need of revitalisation’ was created, describing the healthcare professionals’ experiences with the GC method, 3 years post implementation. The following five categories were found: ‘continuing to facilitate open communication’, ‘expressing a desire for more interprofessional collaboration regarding improvements’, ‘increasing reluctance to report’, ‘downscaling due to the pandemic’ and ‘expressing a desire to share more of what went well’.ConclusionsThis study offers information regarding the healthcare professionals’ experiences with the GC method in a PACU setting; further, it deepens the understanding of the daily patient safety work using this incident reporting method.
Investigation of the self-sealing of Opalinus Clay from the lower sandy facies of Mont Terri site by mock-up tests with highly saline and alkaline solutions
Authors: Barakat Yara , Mokni Nadia , Cui Yu-Jun , Delage Pierre , Bernier Frederic 
Published: May 2023

Three small-scale mock-up tests were carried out on artificially fractured Opalinus Clay samples from the lower sandy facies of Mont Terri site: the first with the synthetic water of neutral pH and at an osmotic suction of 1 MPa, the second with a highly NaNO3 saline solution with a neutral pH and at osmotic suction of 34 MPa, and the third with a hyperalkaline solution at pH = 13.5 and at osmotic suction of 1.16 MPa. The radial swelling pressure was monitored using a total pressure sensor along with the fracture opening using a displacement transducer. The tests ran for over 290 days. After that, the three tests were dismantled. A detailed petrophysical characterization of 45 subsamples was conducted, and the pore size distribution was determined at different positions. X-ray diffractometry measurements were also conducted to identify any mineralogical changes. Results show that, in the case of the synthetic water and the alkaline solution, the friction forces exerted by the lid and the base on the sample were significant and probably hampered the self-sealing of the fracture. Saline solutions affect the self-sealing process of claystones. Indeed, even though increasing the salinity caused the contraction of the diffuse double layer and generated additional fractures within the sample, which increased the permeability and thus accelerated the hydration process, a complete self-sealing of the fracture opening was not observed.
“Anteaters” under the airport: a slender new species of blindsnake, genus Indotyphlops, from Timor-Leste (Scolecophidia: Typhlopidae: Asiatyphlopinae)
Authors: O'Shea Mark , Wallach Van , Hsiao Emma , Kaiser Hinrich 
Published: May 2023

We describe a slender immature female blindsnake from the main airport in Dili, Timor-Leste, as a new species of Indotyphlops, adding a third species to the country's known blindsnake fauna of Sundatyphlops polygrammicus (Schlegel, 1839) and Virgotyphlops braminus (Daudin, 1803). The new species has the following combination of characteristics: small size (snout–vent length = 119 mm), slender body (relative body thickness 71), T-V supralabial imbrication pattern, relative rostral width 0.36, 434 middorsal scales, relative tail length 1.7%, absence of enlarged occipital scales, and apical spine absent. The snake was found in an ant nest under several flat rocks near the fuel depot of Nicolau Lobato International Airport, and this habitat and the discovery in a busy location with heavy human impacts indicate that the species is likely a primarily subterranean myrmeco- or termitophage.
The GENIE BPC NSCLC Cohort: A Real-World Repository Integrating Standardized Clinical and Genomic Data for 1,846 Patients with Non–Small Cell Lung Cancer
Authors: Choudhury Noura J. , Lavery Jessica A. , Brown Samantha , de Bruijn Ino , Jee Justin , Tran Thinh Ngoc , Rizvi Hira , Arbour Kathryn C. , Whiting Karissa , Shen Ronglai , Hellmann Matthew , Bedard Philippe L. , Yu Celeste , Leighl Natasha , LeNoue-Newton Michele , Micheel Christine , Warner Jeremy L. , Ginsberg Michelle S. , Plodkowski Andrew , Girshman Jeffrey , Sawan Peter , Pillai Shirin , Sweeney Shawn M. , Kehl Kenneth L. , Panageas Katherine S. , Schultz Nikolaus , Schrag Deborah , Riely Gregory J. ,   on behalf of the AACR GENIE BPC Core Team
Published: May 2023

Abstract

Purpose:
We describe the clinical and genomic landscape of the non–small cell lung cancer (NSCLC) cohort of the American Association for Cancer Research (AACR) Project Genomics Evidence Neoplasia Information Exchange (GENIE) Biopharma Collaborative (BPC).

Experimental Design:
A total of 1,846 patients with NSCLC whose tumors were sequenced from 2014 to 2018 at four institutions participating in AACR GENIE were randomly chosen for curation using the PRISSMM data model. Progression-free survival (PFS) and overall survival (OS) were estimated for patients treated with standard therapies.

Results:
In this cohort, 44% of tumors harbored a targetable oncogenic alteration, with EGFR (20%), KRAS G12C (13%), and oncogenic fusions (ALK, RET, and ROS1; 5%) as the most frequent. Median OS (mOS) on first-line platinum-based therapy without immunotherapy was 17.4 months [95% confidence interval (CI), 14.9–19.5 months]. For second-line therapies, mOS was 9.2 months (95% CI, 7.5–11.3 months) for immune checkpoint inhibitors (ICI) and 6.4 months (95% CI, 5.1–8.1 months) for docetaxel ± ramucirumab. In a subset of patients treated with ICI in the second-line or later setting, median RECIST PFS (2.5 months; 95% CI, 2.2–2.8) and median real-world PFS based on imaging reports (2.2 months; 95% CI, 1.7–2.6) were similar. In exploratory analysis of the impact of tumor mutational burden (TMB) on survival on ICI treatment in the second-line or higher setting, TMB z-score harmonized across gene panels was associated with improved OS (univariable HR, 0.85; P = 0.03; n = 247 patients).

Conclusions:
The GENIE BPC cohort provides comprehensive clinicogenomic data for patients with NSCLC, which can improve understanding of real-world patient outcomes.
FIGURE 4 from A Very Long-acting Exatecan and Its Synergism with DNA Damage Response Inhibitors
Authors: Fontaine Shaun D. , Carreras Christopher W. , Reid Ralph R. , Ashley Gary W. , Santi Daniel V. 
Published: May 2023

<p><u>PEG-Exa combinations with TLZ or VX970.</u> <b>A,</b> Median relative tumor volume ± interquartile range versus time for PEG-Exa, TLZ, or a combination. The starting tumor volume for all groups was 156 ± 57 mm<sup>3</sup>. Mice (<i>N</i> = 6/group) received a single intraperitoneal dose of vehicle (●), a single intraperitoneal dose of PEG-Exa at 2.5 μmol/kg (▲), QD PO TLZ at 0.4 μmol/kg/day (▲), or a combination of both agents at the same doses (■). <i>P</i> values at 21 days from one-way ANOVA analysis versus vehicle were: 0.002 (PEG-Exa), 0.02 (TLZ) <0.0001 (PEG-Exa + TLZ). <b>B,</b> Median relative tumor volume ± interquartile range versus time for PEG-Exa, VX970 or a combination. The starting tumor volume for all groups was 130 ± 30 mm<sup>3</sup>. Mice (<i>N</i> = 6/group) received a single intraperitoneal dose of vehicle (●), a single intraperitoneal dose of PEG-Exa at 2.5 μmol/kg (▲), orally VX-970 at 64 μmol/kg for 4 consecutive days per week (▲), or a combination of both agents at the same doses (■). The <i>P</i> value at 21 days from one-way ANOVA analysis versus vehicle was 0.006 for the PEG-Exa + VX970 group.</p>