bluuebunny/crossref_metadata_2025_split huggingface dataset:
Remarkable impacts of probiotics supplementation in enhancing of the antioxidant status: results of an umbrella meta-analysis
Authors: Musazadeh Vali , Faghfouri Amir Hossein , Zarezadeh Meysam , Pakmehr Azin , Moghaddam Pooria Taghavi , Hamedi-Kalajahi Fateme , Jahandideh Arian , Ghoreishi Zohreh 
Published: August 2023

IntroductionNumerous meta-analyses have demonstrated the beneficial effects of probiotics on oxidative stress biomarkers, although some studies have contradictory results. Therefore, the current research was conducted to obtain a precise and definite understanding on the impact of probiotics on oxidative stress biomarkers in adults.MethodsWe conducted a comprehensive systematic search of results on Scopus, PubMed, Embase, Web of Science, and Google Scholar dating up to March 2022. Fifteen meta-analyses were included in this umbrella meta-analysis. The random-effects model was employed to obtain the overall effect size. Subgroup analyses were carried out based on supplementation dosage and duration, mean age, and study population.ResultsOur results indicated that probiotics supplementation meaningfully decreased serum malondialdehyde (MDA) (ESWMD = −0.56, 95% CI: −0.72, −0.39; p &lt; 0.001, and ESSMD = −0.50, 95% CI: −0.66, −0.34; p &lt; 0.001). Moreover, the findings showed that probiotics resulted in a significant increase in total antioxidant capacity (TAC) (ESWMD = 29.18, 95% CI: 16.31, 42.04; p &lt; 0.001, and ESSMD = 0.25, 95% CI: 0.02, 0.47; p = 0.032), total glutathione (GSH) (ESWMD: 30.65; 95% CI: 16.94, 44.35, p &lt; 0.001), and nitric oxide (NO) (ESWMD: 1.48; 95% CI: 0.31, 2.65, p = 0.013; I2 = 51.7%, p = 0.043).DiscussionProbiotics could be considered a strong agent in the reinforcement of antioxidant status and preventing the incidence of chronic diseases.
Sucrose-induced hyperglycemia dysregulates intestinal zinc metabolism and integrity: risk factors for chronic diseases
Authors: Mitchell Samuel Blake , Hung Yu-Han , Thorn Trista Lee , Zou Jiaqi , Baser Filiz , Gulec Sukru , Cheung Celeste , Aydemir Tolunay Beker 
Published: August 2023

ObjectiveZinc is an essential micronutrient that is critical for many physiological processes, including glucose metabolism, regulation of inflammation, and intestinal barrier function. Further, zinc dysregulation is associated with an increased risk of chronic inflammatory diseases such as type II diabetes, obesity, and inflammatory bowel disease. However, whether altered zinc status is a symptom or cause of disease onset remains unclear. Common symptoms of these three chronic diseases include the onset of increased intestinal permeability and zinc dyshomeostasis. The specific focus of this work is to investigate how dietary sources of intestinal permeability, such as high sucrose consumption, impact transporter-mediated zinc homeostasis and subsequent zinc-dependent physiology contributing to disease development.MethodWe used in vivo subchronic sucrose treatment, ex vivo intestinal organoid culture, and in vitro cell systems. We analyze the alterations in zinc metabolism and intestinal permeability and metabolic outcomes.ResultsWe found that subchronic sucrose treatment resulted in systemic changes in steady-state zinc distribution and increased 65Zn transport (blood-to-intestine) along with greater ZIP14 expression at the basolateral membrane of the intestine. Further, sucrose treatment enhanced cell survival of intestinal epithelial cells, activation of the EGFR-AKT-STAT3 pathway, and intestinal permeability.ConclusionOur work suggests that subchronic high sucrose consumption alters systemic and intestinal zinc homeostasis linking diet-induced changes in zinc homeostasis to the intestinal permeability and onset of precursors for chronic disease.
Bilateral orbital plasmacytomas as first sign of extramedullary progression post CAR-T therapy: case report and literature review
Authors: Nogués-Castell Javier , Feu-Basilio Silvia , Felguera García Óscar , Fernández de Larrea Carlos , Oliver-Caldés Aina , Balagué Ponz Olga , Fassi Jessica Matas 
Published: August 2023

BackgroundPlasma cell leukemia (PCL) is an aggressive and rare form of plasma cell dyscrasia characterized by peripheral blood expression, poor prognosis, and high relapse rates. Extramedullary plasmacytomas are common in this entity and can affect various organs and soft tissues. Chimeric antigen receptor–T-cell (CAR-T) therapy is a novel immunotherapy for hematological malignancies with promising results. However, it is not indicated for PCL, and experience in this condition is limited. This case is a rare presentation of bilateral orbital plasmacytomas after CAR-T therapy in a patient with PCL history.Case presentationWe present the case of a 51-year-old female patient with a history of previous primary PCL treated with CAR-T therapy achieving complete response and without evidence of systemic progression. Six months after the treatment, she developed subacute proptosis and ptosis on the left eye.An orbital CT scan was performed and showed an orbital tumor in both eyes. A surgical biopsy with histological examination revealed plasma cells, consistent with a plasmacytoma. PET-CT and MRI confirmed the presence of tumors in both orbits. The patient was treated with dexamethasone and chemotherapy along with palliative radiation therapy to the left orbit which had a good response.ConclusionOrbital involvement in multiple myeloma and PCL is rare, with plasmacytomas being more common in other parts of the body. In this report, we present a case of a patient with PCL history, treated with multiple therapeutic lines including CAR-T therapy, who presented bilateral orbital plasmacytomas as the first sign of extramedullary progression after the treatment. This case should be considered by specialist to be aware that the orbits are a possible location of extramedullary progression.
An updated systemic review and meta-analysis on human papillomavirus in breast carcinogenesis
Authors: Awan Usman Ayub , Khattak Aamer Ali , Ahmed Noman , Guo Xingyi , Akhtar Sohail , Kamran Shehrish , Yongjing Zhao , Liu Jianbo , Khan Suliman 
Published: August 2023

BackgroundBreast Cancer (BC) stands out as the widely prevalent malignancy among all the types of cancer affecting women worldwide. There is significant evidence that the pathogenicity of BC may be altered by Human Papillomavirus (HPV) infection; however, conclusive data are not yet available.MethodsBy searching five databases, including EMBASE, IBECS, PubMed, Scopus, Science Direct, Google Scholar, and Web of Science, a thorough systematic analysis was conducted on the prevalence of HPV in BC patients from 1990 to June 30, 2022. After applying extensive eligibility criteria, we selected 74 publications for further analysis based on the prevalence of HPV infections in breast tissues. All of the data were analyzed using a random-effects meta-analysis, Cochran Q test and I2 statistic were used to calculate the heterogeneity of the prevalence among these studies using subgroup analysis. Variations in the HPV prevalence estimates in different subgroups were evaluated by subgroup meta-analysis.ResultsIn total, 3156 studies were initially screened, resulting in 93 full-text studies reviewed, with 74 meeting inclusion criteria. Among a total of 7156 BC biopsies, the pool prevalence of HPV was 25.6% (95% CI= 0.24-0.33, τ2 = 0.0369 with significant heterogeneity between estimates (I2 = 97% and p&lt; 0.01). Consequently, 45 studies with available controls were further studied, and the prevalence of HPV in case-control studies was 26.2% with overall odds 5.55 (95% CI= 3.67-8.41, I2 = 38%, τ2 = 1.4878, p&lt; 0.01). Further subgroup analysis of HPV revealed HPV-16 had a maximum prevalence of 9.6% (95% CI= 3.06-11.86, I2 = 0%, τ2 = 0.6111, p&lt; 0.01). Among different geographical regions, Europe reported the maximum prevalence of HPV, i.e., 39.2% (95% CI=1.29-7.91, I2 = 18%, τ2 = 1.2911, p&lt; 0.01). Overall distribution showed HPV-18 was a frequent HPV subtype reported in Australia.ConclusionCurrent study provides a global estimate of HPV prevalence in BC patients and demonstrates a significant association between this virus and BC etiology. Nevertheless, we recommend further investigation into the underlying mechanism is essential to validate this hypothesis.
The effectiveness of hyperbaric oxygen therapy for managing radiation-induced proctitis – results of a 10-year retrospective cohort study
Authors: Moreira Monteiro António , Alpuim Costa Diogo , Mareco Virgínia , Espiney Amaro Carla 
Published: August 2023

IntroductionDespite modern radiotherapy (RT) techniques, radiation-induced proctitis (RIP) remains a significant complication of RT for pelvic organ malignancies. Over the last decades, an enormous therapeutic armamentarium has been considered in RIP, including hyperbaric oxygen therapy (HBOT). However, the evidence regarding the impact of HBOT on RIP is conflicting. This study aims to evaluate the effectiveness and safety of HBOT in the treatment of RIP.MethodsTen-year (2013-2023) retrospective analysis of all consecutive patients with RIP treated with HBOT at Centro de Medicina Subaquática e Hiperbárica (CMSH) (Armed Forces Hospital – Lisbon, Portugal). Patients were exposed to 100% oxygen at 2.5 ATA, in a multiplace first-class hyperbaric chamber, for 70-min periods, once daily, five times per week. Fisher’s exact test was performed using SPSS (version 23.0); p&lt;0.05 was accepted as statistically significant.ResultsOf a total of 151 patients with RIP, 88 were included in the final analysis, of whom 38.6% evidenced other concurrent radiation-induced soft tissue lesions. The most reported primary pelvic tumor treated with RT was prostate cancer (77.3%), followed by cervical cancer (10.2%). Hematochezia was the most observed clinical manifestation (86.4%). After a median of 60 HBOT sessions (interquartile range [IQR]: 40-87.5), 62.5% and 31.8% of patients achieved a clinical complete and partial response, respectively, with a hematochezia resolution rate of 93.7% (complete or partial). While partial and complete responses require fewer than 70 sessions of HBOT in terms of overall RIP symptoms (p=0.069), isolated hematochezia tends to require at least 70 sessions (p=0.075). Individuals with at least two concurrent late radiation tissue injuries were associated with a complete response to HBOT (p=0.029). Only about 5.7% of patients did not respond to the treatment. Eighteen patients (20.5%) developed reversible ear barotrauma. The number of HBOT sessions was a predictor of HBOT side effects (odds ratio: 1.010; 95% confidence interval, 1.000-1.020; p=0.047).ConclusionThe HBOT proved to be an effective and safe treatment for RIP refractory to medical and/or endoscopic treatments. This real-world evidence study adds value to published data on the management of RIP with HBOT.
Survival of 48866 cancer patients: results from Nantong area, China
Authors: Wang Gao-Ren , Xu Hong , Chen Hai-Zhen , Chen Yong-Sheng , Ni Zhuo-Jian , Fan Li-Yun , Zhang Ai-Hong , Xu Pei-Pei , Qian Yun , Cai Bo , Chen Jian-Guo 
Published: August 2023

ObjectiveThis study aimed to provide a realistic observation of survival by major site for 48,866 cancer patients treated at a tertiary cancer hospital in a rural area of China.MethodsPatients with cancer registered between 2007 and 2017 in the Nantong rural area were followed up. The starting date for survival calculation was the date of the first diagnosis of cancer at the Nantong Tumor Hospital, and the closing date was December 31, 2020. Observed survival (OS) was analyzed according to ICD-10 site, sex, age, region, and hospitalization period using the life table method and compared using the Wilcoxon (Gehan) statistic.ResultsThe overall 5-year OS rate was 40.48% for all 48,866 patients, 30.19% for males, and 51.90% for females. The top five cancer sites, accounting for 60.51% of the total cases, were the esophagus, lung, stomach, liver, and cervix, with 5-year OS rates of 33.72%, 18.64%, 32.10%, 19.04%, and 71.51%, respectively. The highest 5-year OS was observed in the thyroid (87.52%) and the lowest was in the pancreas (6.37%). Survival was significantly higher in younger patients than in older patients, with 5-year OSs of 69.26% and 19.84% in those aged 20-29 and 90-99 years, respectively. Five-year OSs improved significantly from 39.35% in 2007-2011 to 41.26% in 2012-2017.ConclusionOverall survival improved over the years, although the improvement at some sites was not significant. The observed survival varies from region to region, reflecting differences in the patterns of major sites, disparities in proportions of hospitalization, and demographic characteristics.
Mouse maternal odontogenic infection with Porphyromonas gingivalis induces cognitive decline in offspring
Authors: Ishida Eri , Furusho Hisako , Renn Ting-Yi , Shiba Fumie , Chang Hung-Ming , Oue Hiroshi , Terayama Ryuji , Ago Yukio , Tsuga Kazuhiro , Miyauchi Mutsumi 
Published: August 2023

IntroductionPorphyromonas gingivalis (P. gingivalis), a major periodontal pathogen, causes intrauterine infection/inflammation. Offspring exposed to intrauterine infection/inflammation have an increased risk of neurological disorders, regardless of gestational age. However, the relationship between maternal periodontitis and offspring functional/histological changes in the brain has not yet been elucidated.MethodsIn this study, we used a gestational mouse model to investigate the effects of maternal odontogenic infection of P. gingivalis on offspring behavior and brain tissue.ResultsThe step-through passive avoidance test showed that the latency of the acquisition trial was significantly shorter in the P. gingivalis group (p &lt; 0.05), but no difference in spontaneous motor/exploratory parameters by open-field test. P. gingivalis was diffusely distributed throughout the brain, especially in the hippocampus. In the hippocampus and amygdala, the numbers of neuron cells and cyclic adenosine monophosphate response element binding protein-positive cells were significantly reduced (p &lt; 0.05), whereas the number of ionized calcium binding adapter protein 1-positive microglia was significantly increased (p &lt; 0.05). In the hippocampus, the number of glial fibrillary acidic protein-positive astrocytes was also significantly increased (p &lt; 0.05).DiscussionThe offspring of P. gingivalis-infected mothers have reduced cognitive function. Neurodegeneration/neuroinflammation in the hippocampus and amygdala may be caused by P. gingivalis infection, which is maternally transmitted. The importance of eliminating maternal P. gingivalis-odontogenic infection before or during gestation in maintenance healthy brain function in offspring should be addressed in near future.
Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
Authors: Zhang Meiling , Cheng Jinjun , Luo Juan , Li Changxiang , Hou Tingting , Zhao Yan , Wang Yaoxian , Qu Huihua , Kong Hui 
Published: August 2023

As the charcoal processing product of Scutellariae Radix (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon dots (SRC-CDs) were extracted and purified from the aqueous solution of SRC, followed by physicochemical property assessment by series of technologies. The cooling blood and hemostatic effects of SRC-CDs were further evaluated via a blood-heat and hemorrhage (BHH) rat model. Results showed that the diameters of obtained fluorescent SRC-CDs ranged from 5.0 nm to 10.0 nm and possessed functional group-rich surfaces. Additionally, the as-prepared SRC-CDs showed remarkable cooling blood and hemostasis effects in BHH model, mainly manifested by significant improvement of elevated rectal temperature, inflammatory cytokines (TNF-α, IL-6, and IL-1β) levels, as well as protein expressions of myD88 and NF-κB p65, abnormal coagulation parameters (elevated APTT and FIB), hemogram parameters (RBC, HGB, and HCT), and histopathological changes in lung and gastric tissues. This study, for the first time, demonstrated that SRC-CDs were the cooling blood and hemostatic active components of SRC, which could inhibit the release of inflammatory cytokines by regulating myD88/NF-κB signaling pathway, and activating the fibrin system and endogenous coagulation pathway. These results not only provide a new perspective for the study of active ingredients of carbonized herbs represented by SRC, but also lay an experimental foundation for the development of next-generation nanomedicines.
Iron uptake of etioplasts is independent from photosynthesis but applies the reduction-based strategy
Authors: Sági-Kazár Máté , Sárvári Éva , Cseh Barnabás , Illés Levente , May Zoltán , Hegedűs Csaba , Barócsi Attila , Lenk Sándor , Solymosi Katalin , Solti Ádám 
Published: August 2023

IntroductionIron (Fe) is one of themost important cofactors in the photosynthetic apparatus, and its uptake by chloroplasts has also been associated with the operation of the photosynthetic electron transport chain during reduction-based plastidial Fe uptake. Therefore, plastidial Fe uptake was considered not to be operational in the absence of the photosynthetic activity. Nevertheless, Fe is also required for enzymatic functions unrelated to photosynthesis, highlighting the importance of Fe acquisition by non-photosynthetic plastids. Yet, it remains unclear how these plastids acquire Fe in the absence of photosynthetic function. Furthermore, plastids of etiolated tissues should already possess the ability to acquire Fe, since the biosynthesis of thylakoid membrane complexes requires a massive amount of readily available Fe. Thus, we aimed to investigate whether the reduction-based plastidial Fe uptake solely relies on the functioning photosynthetic apparatus.MethodsIn our combined structure, iron content and transcript amount analysis studies, we used Savoy cabbage plant as a model, which develops natural etiolation in the inner leaves of the heads due to the shading of the outer leaf layers.ResultsFoliar and plastidial Fe content of Savoy cabbage leaves decreased towards the inner leaf layers. The leaves of the innermost leaf layers proved to be etiolated, containing etioplasts that lacked the photosynthetic machinery and thus were photosynthetically inactive. However, we discovered that these etioplasts contained, and were able to take up, Fe. Although the relative transcript abundance of genes associated with plastidial Fe uptake and homeostasis decreased towards the inner leaf layers, both ferric chelate reductase FRO7 transcripts and activity were detected in the innermost leaf layer. Additionally, a significant NADP(H) pool and NAD(P)H dehydrogenase activity was detected in the etioplasts of the innermost leaf layer, indicating the presence of the reducing capacity that likely supports the reduction-based Fe uptake of etioplasts.DiscussionBased on these findings, the reduction-based plastidial Fe acquisition should not be considered exclusively dependent on the photosynthetic functions.
Developing a tactical irrigation and nitrogen fertilizer management strategy for winter wheat through drip irrigation
Authors: Zain Muhammad , Si Zhuanyun , Ma Haijiao , Cheng Minghan , Khan Adam , Mehmood Faisal , Duan Aiwang , Sun Chengming 
Published: August 2023

IntroductionAgricultural activities in the North China Plain are often challenged by inadequate irrigation and nutrient supply. Inadequate and improper resource utilization may impose negative impacts on agricultural sustainability. To counteract the negative impacts, a deeper understanding of the different resource management strategies is an essential prerequisite to assess the resource saving potential of crops.MethodsWe explored plausible adaptation strategies including drip irrigation lateral spacings of 40 and 80 cm (hereafter referred to as LS40 and LS80, respectively), irrigating winter wheat after soil water consumption of 20 and 35 mm (hereafter represented as IS20 and IS35, respectively), and nitrogen fertilization scheme of a) applying 50% nitrogen as a basal dose and 50% as a top-dressing dose (NS50:50), b) 25% nitrogen as a basal dose and 75% as a topdressing dose (NS25:75), and c) no nitrogen application as a basal dose and 100% application as a top-dressing dose (NS0:100).Results and discussionThe consecutive 2 years (2017–2018 and 2018–2019) of field study results show that growing winter wheat under LS40 enhanced the water use efficiency (WUE), grain yield, 1,000-grain weight, and number of grains per spike by 15.04%, 6.95%, 5.67%, and 21.59% during the 2017–2018 season, respectively. Additionally, the corresponding values during the 2018–2019 season were 12.70%, 7.17%, 2.66%, and 19.25%, respectively. Irrigation scheduling of IS35 treatment improved all the growth-related and yield parameters of winter wheat. Further, treating 25% nitrogen as a basal dose and application of 75% as a top-dressing dose positively influenced the winter wheat yield. While NS0:100 increased the plant height, leaf area index (LAI), and aboveground biomass as compared to the other application strategies, but high nitrogen was observed in deeper soil layers. Regarding soil environment, the lowest soil moisture and nitrate nitrogen contents were observed in LS80 during both growing seasons. Overall, coupling the IS35 with NS25:75 under 40-cm lateral spacing is a suitable choice for sustainable winter wheat production in theNorth China Plain. The results of our study may be helpful in advancing the knowledge of the farmer community for winter wheat production. The findings can also aid in advancing new insights among scientists working on soil water and nitrogen distribution in drip irrigation for better productivity.
Virtual reality rehabilitation program on executive functions of children with specific learning disorders: a pilot study
Authors: Di Giusto Valentina , Purpura Giulia , Zorzi Carla Fulvia , Blonda Rosanna , Brazzoli Elena , Meriggi Paolo , Reina Tarjn , Rezzonico Silvia , Sala Roberta , Olivieri Ivana , Cavallini Anna 
Published: August 2023

BackgroundThe application of Virtual Reality (VR) in the field of rehabilitation has been widely studied, because it has already proven to be an effective intervention for a variety of physical and cognitive conditions. Nevertheless, its application in pediatric rehabilitation is more recent. This pilot study aims to examine whether a VR-rehabilitation program may have positive effects on the Executive Functions (EFs) of children with Specific Learning Disorders (SLD).Materials and methodsTwenty-four children with diagnosis of SLD participated to the study (range 7–11 years) and performed the VR-training across 6 weeks in the CARE Lab, that was designed with appropriate structural measures and ad hoc fittings, to hide the sophisticated technology necessary to allow the child to experience a rehabilitative setting with recreational and semi-immersive features. Children were evaluated across three main time-points: T0, assessment of cognitive level and EFs immediately before the start of the intervention; T1, assessment of EFs immediately after the end of VR intervention; T2, follow-up of EFs after 6 months from the end of the VR intervention. The rehabilitation programs were customized according to clinical needs and/or single patient’s characteristics, proposing different games with variable complexity levels.ResultsResults showed that scores for visual attention, inhibition, flexibility, and planning abilities were significantly higher than before the intervention, and the most part of these ameliorations were maintained after 6 months.ConclusionThese findings provide important inputs for the development of new innovative rehabilitation interventions for children with SLD that must be founded in ecological and evidence-based approaches.