bluuebunny/crossref_metadata_2025_split huggingface dataset:
mps1andmadmutations reduceCryptococcus neoformanstitan cell viability
Authors: Aktar Koly , Davies Thomas , Leontiou Ioanna , Clark Ivan , Spanos Christos , Wallace Edward , Tuck Laura , Arockia Jeyaprakash A. , Hardwick Kevin G. 
Published: April 2023

AbstractCryptococcus neoformansis an opportunistic, human fungal pathogen which undergoes fascinating switches in cell cycle control and ploidy when it encounters stressful environments such as the human lung. Here we carry out a mechanistic analysis of the spindle assembly checkpoint (SAC) which regulates the metaphase to anaphase transition, focusing on Mps1 kinase and the downstream checkpoint components Mad1 and Mad2. We demonstrate thatCryptococcus mad1Δormad2Δstrains are unable to respond to microtubule perturbations, continuing to re-bud and divide, and die rapidly as a consequence. Fluorescent tagging of Chromosome 3, using a lacO array and mNeonGreen-lacI fusion protein, demonstrates thatmadmutants are unable to maintain sister-chromatid cohesion in the absence of microtubule polymers. Thus, the classic checkpoint functions of the SAC are conserved inCryptococcus. In interphase, GFP-Mad1 is enriched at the nuclear periphery, and it is recruited to unattached kinetochores in mitosis. Purification of GFP-Mad1 followed by mass spectrometric analysis of associated proteins show that that it forms a complex with Mad2 and that it interacts with other checkpoint signalling components (Bub1) and effectors (Cdc20 and APC/C sub-units) in mitosis. We also demonstrate that overexpression of Mps1 kinase is sufficient to arrestCryptococcuscells in mitosis, and show that this arrest is dependent on both Mad1 and Mad2. We find that a C-terminal fragment of Mad1 is an effectivein vitrosubstrate for Mps1 kinase and map several Mad1 phosphorylation sites. Some sites are highly conserved within the C-terminal Mad1 structure and we demonstrate that mutation of threonine 667 (T667A) leads to loss of checkpoint signalling and abrogation of theGAL-MPS1arrest. Thus Mps1-dependent phosphorylation of C-terminal Mad1 residues is a critical step inCryptococcusspindle checkpoint signalling. Finally, we analyse the phenotype ofmadandmps1mutants during titan cell generation: quantitating viability of titan cells and their daughters generated during the ensuing reductive division. Themad1Δ, mad2ΔandmpsΔmutants show significantly reduced viability: many titans are dead and others produce slow growing colonies. We propose that theseCryptococcus neoformanscheckpoint proteins have important roles in ensuring high fidelity chromosome segregation during stressful conditions, such that those heightened during its polyploid infection cycle.
Global Profiling of Differentiating Macrophages Identifies Novel Functional Long Non-coding RNAs Regulating Polarization and Innate Immune Responses
Authors: Valverde Araceli M. , Naqvi Raza A. , Naqvi Afsar R. 
Published: April 2023

AbstractMacrophages (Mφ) are functionally dynamic immune cells that bridge innate and adaptive immune responses. However, the underlying epigenetic mechanisms that control the macrophage plasticity and innate immune functions are not well-elucidated. Here we performed transcriptome profiling of differentiating M1Mφ and M2Mφ and identified thousands of previously known and novel lncRNAs. We characterized three Mφ-enriched lncRNAs (LRRC75A-As1, GAPLINC and AL139099.5) with novel functions in Mφ differentiation, polarization and innate immunity. Knockdown of LRRC75A-As1, and GAPLINC downregulated Mφ differentiation markers CDw93 and CD68, and skewed macrophage polarization by decreasing M1 markers but had no significant impact on M2 markers. LRRC75A-As1, and GAPLINC RNAi in Mφ attenuated bacterial phagocytosis, antigen processing and inflammatory cytokine secretion supporting their functional role in potentiating innate immune functions. Mechanistically, lncRNA knockdown perturbed the expression of multiple cytoskeleton signaling thereby impairing Mφ migration suggesting their critical role in regulating macrophage polarity and motility. Together, our results show that Mφ acquire a unique repertoire of lncRNAs to shape differentiation, polarization and innate immune functions.
Simultaneous representation of multiple time horizons by entorhinal grid cells and CA1 place cells
Authors: Chaudhuri-Vayalambrone Prannoy , Rule Michael , Bauza Marius , Krstulovic Marino , Kerekes Pauline , Burton Stephen , O’Leary Timothy , Krupic Julija 
Published: April 2023

SummaryGrid cells and place cells constitute the basic building blocks of the medial entorhinal-hippocampal spatial cognitive map by representing the spatiotemporal continuum of an animal’s past, present and future locations. However, the spatiotemporal relationship between these different cell types is unclear. Here we co-recorded grid and place cells in freely foraging rats. We show that average time shifts in grid cells tend to be prospective and are proportional to their spatial scale, providing a nearly instantaneous readout of a spectrum of progressively increasing time horizons ranging hundreds of milliseconds. Average time shifts of place cells are generally larger compared to grid cells and also increase with place field sizes. Moreover, time shifts displayed nonlinear modulation by the animal’s trajectories in relation to the local boundaries and locomotion cues. Finally, long and short time shifts occurred at different parts of the theta cycle, which may facilitate their readout. Together, these findings suggest that progressively increasing time horizons of grid and place cells may provide a basis for calculating animal trajectories essential for goal-directed navigation and planning.
Tension between the safe flow of government data across organizational boundaries and fragmentations in secure collaboration: the Chinese e-government
Authors: Mao Zijun , Zhu Yuqian 
Published: April 2023

PurposeThe study focuses on influential factors of collaboration on government data security by the Chinese government.Design/methodology/approachThe article explores the case of e-government in the Chinese centralized unitary state system context, using a structured–pragmatic–situational (SPS) approach and the boundary theory as an analytical lens.FindingsThe findings indicate that e-government operates in highly interconnected environments where the safe flow of government data requires collaborative and cross-boundary strategies. Any organization is a potential “weakest link”. In addition, collaboration is fragmented by ambiguous accountability and organizational inertia across government departments, resources differences and limited visibility and measurability of security efforts across government levels and conflicts and uncertainties in principal–agent relationships. The solutions for those obstacles are also discussed from the multi-function, multi-level and multi-actor dimensions, respectively. A multi-dimensional overarching security model for the flow of government data is proposed.Originality/valueThe study advances the technology-oriented micro-analysis of previous studies on government data security to cross-organizational revealing at the macrolevel by connecting streams of research in information systems and public administration. These findings will contribute to making the safe flow of government data more resilient in the transformation of e-government.
Managing effective labor market entry: proactive coping and self-employment intention among Polish small business owners
Authors: Ślebarska Katarzyna , Stremska Aneta , Kowalski Grzegorz 
Published: April 2023

PurposeSelf-employment is considered one of the responses to precarious employment, particularly among those who lost jobs during an economic crisis. Although starting a new business is widely available, operating new ventures remains challenging. This article aims to explore the premises of self-employment success, namely self-employment intention and proactive coping as crucial predictors of further performance.Design/methodology/approachThe authors recruited participants among initially unemployed individuals who participated in the entrepreneurial program aimed at creating self-employment. Since entrepreneurs are expected to have specific personal characteristics important for performance, the authors assessed proactive coping as the key factor for self-employment intention.FindingsThe results depicted proactive coping as crucial in performing own ventures in the long run, which suggests that self-employment intention may change over time.Practical implicationsProactive coping is particularly appropriate for self-employed at any stage of the entrepreneurial process because it maintains the intention to perform own business. Thus, the findings underline the need for proactive coping training for entrepreneurs, particularly those previously unemployed.Originality/valueAs the self-employment intention may differ in time, the importance of being proactive in operating small businesses increases.
The impact of mutual funds’ ESG scores on their financial performance during the COVID-19 pandemic. A data envelopment analysis
Authors: Tampakoudis Ioannis , Kiosses Nikolaos , Petridis Konstantinos 
Published: April 2023

Purpose
The purpose of this study is to evaluate the performance of mutual funds during the COVID-19 pandemic with environmental, social and governance (ESG) criteria. The main research question is whether mutual fund performance differs with respect to the level of the mutual fund’s ESG score.

Design/methodology/approach
The data set contains global fund data, and mutual fund performance is analyzed using two types of data envelopment analysis (DEA) models: the DEA portfolio index (DPEI) and the range direction measure (RDM) DEA. Propensity score matching and logistic regression are also applied.

Findings
The results reveal that: nonequity mutual funds present significantly higher performance compared to the performance of equity mutual funds; mutual funds with high ESG scores are associated with significantly higher performance compared to those with low to medium ESG scores; funds with high ESG scores experience higher performance irrespective of their type; and efficiency scores derived from the RDM DEA are significantly higher than those derived from the DPEI model.

Research limitations/implications
Investors, fund managers and market participants can benefit from the findings of this study and improve their investment decision-making process, including more sustainable funds in their portfolios. Regulators and policymakers should further promote or even require the inclusion of more sustainable investments in the financial products offered by institutional investors. The main limitation of the study is related to data availability regarding the ESG score of mutual funds.

Originality/value
To the best of the authors’ knowledge, this is the first study that provides robust evidence in support of a positive association between ESG scores and mutual fund performance during the pandemic-induced crisis applying a DEA methodology.
Enterprise digitalization, employee digital literacy and R&D cooperation: the moderating role of organizational inertia
Authors: Lei Hui , Tang Shiyi , Zhao Yuxin , Chen Shou 
Published: April 2023

Purpose
This study aims to explore the effect of digitalization on the promotion of enterprise R&D cooperation, and it analyzes the microimpact mechanism and boundary conditions of enterprise digitalization on enterprise R&D cooperation.

Design/methodology/approach
Based on survey data sourced from the World Bank Enterprise Surveys of the business environment of Chinese enterprises in 2012, this study applies multiple regression methods to test theoretical hypotheses.

Findings
Enterprise digitalization positively affects the breadth and intensity of enterprise R&D cooperation. Employees’ digital literacy plays an intermediary role between enterprise digitalization and enterprise R&D cooperation. The subordinate attributes of enterprises weaken the positive relationship between enterprise digitalization and the breadth and intensity of enterprise R&D cooperation. The shareholding of state-owned enterprises reinforces the positive relationship between digitalization and the intensity of enterprise R&D cooperation. However, such shareholding shows no significant regulatory effect on digitalization and the breadth of enterprise R&D cooperation.

Originality/value
Focusing on the digital transformation of the enterprise, this study discusses its impact mechanism on enterprise R&D cooperation, including the impact on the intensity and breadth of R&D cooperation. The study further examines the regulatory effect of organizational inertia on enterprise digital and R&D cooperation from two aspects: resource rigidity and routine rigidity. It emphasizes the significance of the digital literacy of employees in enterprise digitalization and discusses the micromechanism of enterprise digitalization and enterprise R&D cooperation.
The role of crowdfunding in endorsing responsible open innovation for shared value co-creation: a systematic literature review
Authors: Cillo Valentina , Borin Elena , Thomas Asha , Chaturvedi Anurag , Faggioni Francesca 
Published: April 2023

PurposeThis paper aims to investigate the intersection between crowdfunding (CF), open innovation (OI) and responsible innovation (RI) and identify the emerging trends and gaps in research and new paths for CF research in the future. In addition, this paper proposes a conceptual framework and propositions.Design/methodology/approachThis paper is structured in line with the systematic literature review protocol. After reading all the titles, keywords and abstracts, 172 papers focused on OI and RI were selected for this research. Finally, 27 papers that are based on dimensions related to responsible OI were selected for the study.FindingsDue to CF's multidisciplinary nature, the scientific literature on the role of CF in endorsing responsible OI for shared value co-creation appears fragmented and redundant. Several emerging trends and gaps of research and new paths for CF research in the future arise regarding research methodology and theoretical perspective.Originality/valueTo the best of the authors' knowledge, this is the first study investigating the intersection between CF OI and RI.
Hedging potentials of green investments against climate and oil market risks
Authors: Adediran Idris A. , Swaray Raymond , Orekoya Aminat O. , Kabir Balikis A. 
Published: April 2023

PurposeThis study aims to examine the ability of clean energy stocks to provide cover for investors against market risks related to climate change and disturbances in the oil market.Design/methodology/approachThe study adopts the feasible quasi generalized least squares technique to estimate a predictive model based on Westerlund and Narayan’s (2015) approach to evaluating the hedging effectiveness of clean energy stocks. The out-of-sample forecast evaluations of the oil risk-based and climate risk-based clean energy predictive models are explored using Clark and West’s model (2007) and a modified Diebold & Mariano forecast evaluation test for nested and non-nested models, respectively.FindingsThe study finds ample evidence that clean energy stocks may hedge against oil market risks. This result is robust to alternative measures of oil risk and holds when applied to data from the COVID-19 pandemic. In contrast, the hedging effectiveness of clean energy against climate risks is limited to 4 of the 6 clean energy indices and restricted to climate risk measured with climate policy uncertainty.Originality/valueThe study contributes to the literature by providing extensive analysis of hedging effectiveness of several clean energy indices (global, the United States (US), Europe and Asia) and sectoral clean energy indices (solar and wind) against oil market and climate risks using various measures of oil risk (WTI (West Texas intermediate) and Brent volatility) and climate risk (climate policy uncertainty and energy and environmental regulation) as predictors. It also conducts forecast evaluations of the clean energy predictive models for nested and non-nested models.
A comparison of public perceptions of cisgender male and transgender male stalking perpetrators
Authors: Cook Lydia Caroline , Duff Simon C. 
Published: April 2023

Purpose
The purpose of this study is to compare perceptions of male cisgender and male transgender stalking perpetrators. There present study compared participants’ perceptions of whether behaviour constituted stalking, posed a threat, had a risk of violence and required police intervention when the perpetrator was transgender or cisgender. The present study also sought to replicate the prior-relationship misconception in stalking literature and to investigate whether perceptions of transgender perpetrators changed based on the age and gender of the perceiver.

Design/methodology/approach
Participants read vignettes outlining the relationship between victim and perpetrator as well as a description of the stalking behaviours. Participants then reported their perceptions of the four dependent variables on Likert-type scales.

Findings
The prior-relationship misconception was replicated. There were no significant differences in perceptions of transgender and cisgender perpetrators across the four dependent variables. There were also no significant differences in perceptions based on the gender of the perceiver. Contrary to expectations, older participants perceived transgender perpetrators as less threatening than younger participants.

Research limitations/implications
The prior-relationship misconception is robust to gender identity of the perpetrator. The participants in the present study seemed to make judgements based on stalking behaviour and not the gender identity of the perpetrator. Future research should replicate this study with more severe stalking behaviours and with greater variation in gender identity.

Originality/value
To the best of the authors’ knowledge, this study is the first to compare perceptions of cisgender and transgender males in the context of stalking perpetration. There is also consideration of how the demographics of the perceiver could impact these perceptions. This study also contributes to research on the prior-relationship misconception by demonstrating that the misconception is robust to gender identification of the perpetrator.
The abuse of the beneficial ownership of trusts to conceal assets in insolvency and divorce proceedings: a South African study
Authors: Dubber Andrea , Van Graan Constant , Groenewald Andre 
Published: April 2023

Purpose
Previous research has indicated that trusts are used to commit various economic crimes, but limited studies examine the exact method of how trusts are abused. This paper aims to determine how trusts are abused to conceal assets in insolvency and divorce proceedings. Apart from discussing how fraudulent trusts are evaluated by South African courts, two court cases will also be analysed to determine how trusts have been abused in the past to conceal assets in insolvency and divorce proceedings.

Design/methodology/approach
The methodology used is a literature study, predominantly using court cases and relevant statutes as the primary sources of information. The difference between a sham and alter ego trust is discussed, whereafter two court cases are dissected to identify how trusts have been abused to conceal assets.

Findings
The study found that trusts can be abused in different ways to conceal assets in insolvency and divorce proceedings. This can vary from the way the trust is established to the way the trust is used. But trusts are particularly susceptible to abuse when there is no separation between the ownership and enjoyment of trust assets, and the trust lacks independent trustees.

Originality/value
The research finding can be used to better understand how trusts are abused in divorce and insolvency proceedings.
How does knowledge hiding play a role in the relationship between leader–member exchange differentiation and employee creativity? A cross-level model
Authors: Liao Ganli , Li Mengyao , Li Yi , Yin Jielin 
Published: April 2023

Purpose
Employees’ knowledge management, which influences creativity, is a pivotal resource in organizational innovation activities, as it helps activate the knowledge resource pool and improves knowledge flow. Using social information processing theory, this study aims to construct a cross-level model to examine how knowledge hiding plays a role in the relationship between leader–member exchange differentiation (LMXD) and employee creativity.

Design/methodology/approach
This study surveyed 754 leader–employee matching samples from 127 teams in China innovation enterprises at two time points. Confirmatory factor analysis, convergent analysis, hierarchical regression analysis and bootstrapping method by SPSS and AMOS were used to test the hypotheses.

Findings
The empirical results demonstrate the cross-level model’s efficiency and reveal the following findings: Team-level LMXD is negatively related to employee creativity, whereas it is positively related to knowledge hiding; knowledge hiding is negatively associated with employee creativity; thus, knowledge hiding plays a mediating role in the relationships between them.

Originality/value
Based on the knowledge-hiding perspective, this study analyzed an underlying mechanism between LMXD and employee creativity, thereby further enriching the literature on the influence of knowledge management. This proposed connection has not been established previously. Moreover, the findings respond to the reasons for the inconsistent conclusions of previous literature on the cross-level relationship between LMXD and employee creativity based on the social information processing theory. It thus clarifies the cross-level influence path, as well as provides a theoretical basis for further research on the relationship between the two.
Peer stigma, consequences, and anti-stigma strategies in adolescents with mental disorders: a qualitative investigation
Authors: Aiyub Aiyub , Jannah Syarifah Rauzatul , Marthoenis Marthoenis , Abdullah Asnawi , Sofyan Hizir 
Published: April 2023

Purpose
This paper aims to investigate the local perspectives, based on culture and beliefs about peer stigma, consequences and strategies to reduce mental health stigma among adolescents in Indonesia.

Design/methodology/approach
This study included 30 participants, including students, teachers, health professionals and lecturers. Two sessions of focus group discussions and 14 sessions of in-depth interviews were performed to collect the data. A qualitative content analysis was carried out, and some related themes and sub-themes were explored.

Findings
This study found that peer stigma was prevalent and negatively impacted the psychological, social and intellectual development of adolescents with mental disorders (MD) in Indonesian society. Five strategies to tackle peer stigma were proposed, including increasing mental health literacy (MHL), reducing the harmful effects of stigma, fostering a supportive social environment, expanding access to and improving the quality of mental health care and advocating for public health policies.

Research limitations/implications
More qualitative research on the experiences and perspectives of victims, perpetrators and society concerning mental health stigma in adolescents is required to fully understand why stigma arises, what causative factors and repercussions it has, as well as how to deal with it.

Practical implications
Peer stigma still exists among adolescents. It has negative impacts on their mental and psychological well-being. MHL, social environment, the harmful effects of stigma, as well as mental health access and policy were concerns of this study. A school is a place where adolescents spend most of their social time. Schools play a vital part in the stigma-reduction program.

Originality/value
Mental health stigma among adolescents with MD is an issue that has potentially hindered mental health recovery. Anti-stigma intervention based on local perspectives is valuable in increasing the effectiveness and acceptance of interventions.