bluuebunny/crossref_metadata_2025_split huggingface dataset:
Schizophrenia and cardiometabolic abnormalities: A Mendelian randomization study
Authors: Saadullah Khani Noushin , Cotic Marius , Wang Baihan , Abidoph Rosemary , Mills Georgina , Richards-Belle Alvin , Perry Benjamin I. , Khandaker Golam M. , Bramon Elvira 
Published: April 2023

Background: Individuals with a diagnosis of schizophrenia are known to be at high risk of premature mortality due to poor physical health, especially cardiovascular disease, diabetes, and obesity. The reasons for these physical health outcomes within this patient population are complex. Despite well-documented cardiometabolic adverse effects of certain antipsychotic drugs and lifestyle factors, schizophrenia may have an independent effect.Aims: To investigate if there is evidence that schizophrenia is causally related to cardiometabolic traits (blood lipids, anthropometric traits, glycaemic traits, blood pressure) and vice versa using bi-directional two-sample Mendelian randomization (MR) analysis.Methods: We used 185 genetic variants associated with schizophrenia from the latest Psychiatric Genomics Consortium GWAS (n = 130,644) in the forward analysis (schizophrenia to cardiometabolic traits) and genetic variants associated with the cardiometabolic traits from various consortia in the reverse analysis (cardiometabolic traits to schizophrenia), both at genome-wide significance (5 × 10−8). The primary method was inverse-variance weighted MR, supported by supplementary methods such as MR-Egger, as well as median and mode-based methods.Results: In the forward analysis, schizophrenia was associated with slightly higher low-density lipoprotein (LDL) cholesterol levels (0.013 SD change in LDL per log odds increase in schizophrenia risk, 95% CI, 0.001–0.024 SD; p = 0.027) and total cholesterol levels (0.013 SD change in total cholesterol per log odds increase in schizophrenia risk, 95% CI, 0.002–0.025 SD; p = 0.023). However, these associations did not survive multiple testing corrections. There was no evidence of a causal effect of cardiometabolic traits on schizophrenia in the reverse analysis.Discussion: Dyslipidemia and obesity in schizophrenia patients are unlikely to be driven primarily by schizophrenia itself. Therefore, lifestyle, diet, antipsychotic drugs side effects, as well as shared mechanisms for metabolic dysfunction and schizophrenia such as low-grade systemic inflammation could be possible reasons for the apparent increased risk of metabolic disease in people with schizophrenia. Further research is needed to examine the shared immune mechanism hypothesis.
Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury
Authors: Zhang Yifei , Chen Chun , Li Dawei , Chen Penghui , Hang Lei , Yang Jun , Xie Jin 
Published: April 2023

Specific biomarkers of ferroptosis after peripheral nerve injury (PNI) are still under debate. In this study, 52 differentially expressed ferroptosis-related genes (DE-FRGs) were retrieved from publicly accessible sequencing data of intact and injured samples of rats with sciatic nerve crush injury. Functional enrichment analyses revealed that adipogenesis, mitochondrial gene sets, and pathways of MAPK, p53, and CD28 family were predominantly engaged in ferroptosis after PNI. Next, Cdkn1a, Cdh1, Hif1a, Hmox1, Nfe2l2, and Tgfb1 were investigated as new ferroptosis-associated hub genes after PNI. Subsequently, clustering correlation heatmap shows six hub genes are linked to mitochondria. The immunofluorescence assay at 0, 1, 4, 7, and 14 days indicated the temporal expression patterns of Tgfb1, Hmox1, and Hif1a after PNI were consistent with ferroptosis validated by PI and ROS staining, while Cdh1, Cdkn1a, and Nfe2l2 were the opposite. In summary, this study identified six hub genes as possible ferroptosis-related biomarkers for PNI, which may offer therapeutic targets for peripheral nerve regeneration and provide a therapeutic window for ferroptosis.
Prognostic value of various immune cells and Immunoscore in triple-negative breast cancer
Authors: Ren Xinyu , Song Yu , Pang Junyi , Chen Longyun , Zhou Liangrui , Liang Zhiyong , Wu Huanwen 
Published: April 2023

BackgroundThis study aimed to evaluate the expression status and prognostic role of various immunoregulatory cells and test in triple-negative breast cancer (TNBC).MethodsThe expression of five markers (CD3/CD4/CD8/CD19/CD163) of tumor immune cells was evaluated retrospectively in tumor sections from 68 consecutive cases of TNBC by immunohistochemistry. Computational image analysis was used to quantify the density and distribution of each immune marker within the tumor region, tumor invasive margin, and expression hotspots. Immunoscores were calculated using an automated approach. Other clinical characteristics were also analyzed.ResultsFor all patients, Kaplan–Meier survival analysis showed that high CD3+ signals in the tumor region (disease-free survival (DFS), P=0.0014; overall survival (OS), P=0.0031) and total region (DFS, P=0.0014; OS, P=0.0031) were significantly associated with better survival. High CD4+ levels in the tumor region and total regions were significantly associated with better survival (P&lt;0.05). For Hotspot analysis, CD3+ was associated with significantly better survival for all Top1, Top2, and Top3 densities (DFS and OS, P&lt;0.05). High CD4+ levels were significantly associated with better prognosis for Top1 and Top3 densities (DFS and OS, P&lt;0.05). For stage IIB and IIIC patients, CD3+ in the tumor region and all Top hotspots was found to be significantly correlated with survival (DFS and OS, P&lt;0.05). CD4+ cells were significantly associated with survival in the tumor region, total region, and Top3 density (DFS, P=0.0213; OS, P=0.0728). CD8+ cells were significantly associated with survival in the invasive margin, Top2 density, and Top3 density. Spatial parameter analysis showed that high colocalization of tumor cells and immune cells (CD3+, CD4+, or CD8+) was significantly associated with patient survival.ConclusionComputational image analysis is a reliable tool for evaluating the density and distribution of immune regulatory cells and for calculating the Immunoscore in TNBC. The Immunoscore retains its prognostic significance in TNBC later than IIB stage breast cancer. Future studies are required to confirm its potential to predict tumor responses to chemotherapy and immune therapy.
Inflammation-related proteomics demonstrate landscape of fracture blister fluid in patients with acute compartment syndrome
Authors: Long Yubin , Li Yiran , Wang Tao , Ni Andrew , Guo Jialiang , Dong Qi , Yang Shuo , Guo Junfei , Wang Ling , Hou Zhiyong 
Published: April 2023

BackgroundBlisters are tense vesicles or bullae that arise on swollen skin and are found in a wide range of injuries. As a complication of fracture, fracture blisters are considered soft tissue injuries, which often lead to adverse effects such as prolonged preoperative waiting time and increased risk of surgical site infection. However, our previous study found that in patients with acute compartment syndrome, fracture blisters may be a form of compartment pressure release, but the specific mechanism has not been revealed. Here, we mapped out the proteomic landscape of fracture blister fluid for the first time and compared its expression profile to cupping and burn blisters.MethodsFirst, fluid samples were collected from 15 patients with fracture blisters, 7 patients with cupping blisters, and 9 patients with burn blisters. Then, the expression levels of 92 inflammatory proteins were measured using the Olink Target 96 Inflammation panel. Protein profiles were compared across the three groups using Differential Protein Expression Analysis and Principal Component Analysis (PCA).ResultsFracture blisters had significantly higher levels of 50 proteins in comparison to cupping and 26 proteins in comparison to burn blisters. Notably, PCA showed fracture blisters closely resembled the protein expression profile of burn blisters but were distinct from the protein expression profile of cupping blisters.ConclusionOur study provides the first characterization of fracture blister fluid using proteomics, which provides a valuable reference for further analysis of the difference between blisters caused by fractures and those caused by other pathogenic factors. This compendium of proteomic data provides valuable insights and a rich resource to better understand fracture blisters.
Single-cell immune profiling reveals immune responses in oral lichen planus
Authors: Li Qionghua , Wang Fei , Shi Yujie , Zhong Liang , Duan Shumin , Kuang Wenjing , Liu Na , Luo En , Zhou Yu , Jiang Lu , Dan Hongxia , Luo Xiaobo , Zhang Dunfang , Chen Qianming , Zeng Xin , Li Taiwen 
Published: April 2023

IntroductionOral lichen planus (OLP) is a common chronic inflammatory disorder of the oral mucosa with an unclear etiology. Several types of immune cells are involved in the pathogenesis of OLP.MethodsWe used single-cell RNA sequencing and immune repertoire sequencing to characterize the mucosal immune microenvironment of OLP. The presence of tissue-resident memory CD8+ T cells are validated by multiplex immunofluorescence.ResultsWe generated a transcriptome atlas from four OLP biopsy samples and their paired peripheral blood mononuclear cells (PBMCs), and compared them with two healthy tissues and three healthy PBMCs samples. Our analysis revealed activated tissue-resident memory CD8+ T cells in OLP tissues. T cell receptor repertoires displayed apperant clonal expansion and preferrential gene pairing in OLP patients. Additionally, obvious BCR clonal expansion was observed in OLP lesions. Plasmacytoid dendritic cells, a subtype that can promote dendritic cell maturation and enhance lymphocyte cytotoxicity, were identified in OLP. Conventional dendritic cells and macrophages are also found to exhibit pro-inflammatory activity in OLP. Cell-cell communication analysis reveals that fibroblasts might promote the recruitment and extravasation of immune cells into connective tissue.DiscussionOur study provides insights into the immune ecosystem of OLP, serving as a valuable resource for precision diagnosis and therapy of OLP.
Construction of BaTiO3/CeO2 heterojunction and comparative study on photocatalytic activity of degradation of different drugs
Authors: Ye Chao , Li Chengui 
Published: April 2023

A type II band aligned BaTiO3/CeO2 photocatalysts was constructed by a simple one-step solution synthesis method. The BaTiO3/CeO2 photocatalysts contain only cubic phase BaTiO3 and CeO2, and the particles are approximately spherical with a small amount of adhesion and agglomeration, and the average particle size is about 50 nm. Transmission electron microscopy (TEM) observation and element Mapping characterization confirmed the formation of special heterojunction between BaTiO3 and CeO2 and the presence of a large number of oxygen vacancies, which resulted in high visible optical absorption coefficient. The BaTiO3/CeO2 photocatalysts demonstrated high photocatalytic activity for the degradation of oxytetracycin hydrochloride, and the optimal drug concentration, catalyst content and pH value are 50 mg/L, 1 g/L and 7, respectively. Simultaneously, the BaTiO3/CeO2 photocatalysts were selective for degradation of aureomycin hydrochloride, doxycycline hydrochloride and tetracycline hydrochloride. Other photocatalytic experiments show that the BaTiO3/CeO2 photocatalysts have high chemical and cyclic stability, and the synergistic effect of holes, hydroxyl radicals and superoxide radicals plays an important role in the whole photocatalytic process. This simple synthesis route provides a technical reference for constructing other wide-band gap semiconductors to respond to simulated sunlight.
A retrospective analysis of normal saline and lactated ringers as resuscitation fluid in sepsis
Authors: Isha Shahin , Satashia Parthkumar H. , Yarrarapu Siva Naga S. , Govero Austin B. , Harrison Michael F. , Baig Hassan Z. , Guru Pramod , Bhattacharyya Anirban , Ball Colleen T. , Caples Sean M. , Grek Ami A. , Vizzini Michael R. , Khan Syed Anjum , Heise Katherine J. , Sekiguchi Hiroshi , Cantrell Warren L. , Smith Jeffrey D. , Chaudhary Sanjay , Gnanapandithan Karthik , Thompson Kristine M. , Graham Charles G. , Cowdell Jed C. , Murawska Baptista Aleksandra , Libertin Claudia R. , Moreno Franco Pablo , Sanghavi Devang K. 
Published: April 2023

BackgroundThe Surviving Sepsis Campaign suggested preferential resuscitation with balanced crystalloids, such as Lactated Ringer’s (LR), although the level of recommendation was weak, and the quality of evidence was low. Past studies reported an association of unbalanced solutions, such as normal saline (NS), with increased AKI risks, metabolic acidosis, and prolonged ICU stay, although some of the findings are conflicting. We have compared the outcomes with the preferential use of normal saline vs. ringer’s lactate in a cohort of sepsis patients.MethodWe performed a retrospective cohort analysis of patients visiting the ED of 19 different Mayo Clinic sites between August 2018 to November 2020 with sepsis and receiving at least 30 mL/kg fluid in the first 6 h. Patients were divided into two cohorts based on the type of resuscitation fluid (LR vs. NS) and propensity-matching was done based on clinical characteristics as well as fluid amount (with 5 ml/kg). Single variable logistic regression (categorical outcomes) and Cox proportional hazards regression models were used to compare the primary and secondary outcomes between the 2 groups.ResultsOut of 2022 patients meeting our inclusion criteria; 1,428 (70.6%) received NS, and 594 (29.4%) received LR as the predominant fluid (&gt;30 mL/kg). Patients receiving predominantly NS were more likely to be male and older in age. The LR cohort had a higher BMI, lactate level and incidence of septic shock. Propensity-matched analysis did not show a difference in 30-day and in-hospital mortality rate, mechanical ventilation, oxygen therapy, or CRRT requirement. We did observe longer hospital LOS in the LR group (median 5 vs. 4 days, p = 0.047 and higher requirement for ICU post-admission (OR: 0.70; 95% CI: 0.51–0.96; p = 0.026) in the NS group. However, these did not remain statistically significant after adjustment for multiple testing.ConclusionIn our matched cohort, we did not show any statistically significant difference in mortality rates, hospital LOS, ICU admission after diagnosis, mechanical ventilation, oxygen therapy and RRT between sepsis patients receiving lactated ringers and normal saline as predominant resuscitation fluid. Further large-scale prospective studies are needed to solidify the current guidelines on the use of balanced crystalloids.
Incidence of cutaneous melanoma and Merkel cell carcinoma in patients with primary cutaneous B-cell lymphomas: A population study of the SEER registry
Authors: Banner Lauren , Joffe Daniel , Lee Emily , Porcu Pierluigi , Nikbakht Neda 
Published: April 2023

IntroductionThe increased incidence of cutaneous melanoma (CM) and Merkel cell carcinoma (MCC) in patients with hematologic malignancies (HM) is well established. While the risk of CM has been assessed in some subtypes of HM including cutaneous T-cell lymphoma, the incidence in patients with primary cutaneous B-cell lymphoma (PCBCL) has not been interrogated.MethodsHere we evaluated the standardized incidence ratio (SIR) of CM and MCC in 5,179 PCBCL patients compared to approximately 1.5 billion individuals in the general population using the Surveillance, Epidemiology and End Results (SEER) database. Among patients with PCBCL, we identified subgroups that were at increased risk for CM or MCC as a second primary cancer.ResultsWe found 36 cases of CM in the PCBCL cohort (SIR, 1.35; 95% CI, 0.94–1.86), among which SIR was significantly elevated for non-Hispanic White patients compared to the general population (SIR, 1.48; 95% CI, 1.03–2.06). Males had a significantly increased risk of developing CM after a diagnosis of PCBCL (SIR, 1.60; 95% CI, 1.10–2.26). We found that males in the age group of 50–59 were at increased risk for CM development (SIR, 3.02; 95% CI, 1.11–6.58). Males were at increased risk of CM 1–5 years after PCBCL diagnosis (SIR, 2.06; 95% CI, 1.18–3.34). Patients were at greater risk of developing MCC within 1 year of diagnosis of PCBCL (SIR, 23.60; 95% CI, 2.86–85.27), particularly in patients who were over the age of 80 (SIR, 46.50; 95% CI, 5.63–167.96). Males aged 60–69 with PCBCL, subtype marginal zone, were also at increased risk for MCC (SIR, 42.71; 95% CI, 1.08–237.99).ConclusionThere is an increased incidence of CM in White, middle-aged males within 5 years of diagnosis of PCBCL and an increased risk of MCC in elderly patients within 1 year of PCBCL diagnosis. These data suggest that certain subgroups of patients with PCBCL may require more rigid surveillance for CM and MCC.
Spatio-temporal pattern of short birth interval and associated factors on women in Ethiopia: Using Ethiopian demographic and health surveys 2000–2016
Authors: Arega Gezachew G. , Mitku Aweke A. , Fenta Haile M. 
Published: April 2023

BackgroundA short birth interval is a critical factor that contributes to a large number of maternal and infant mortality in low- and middle-income countries. It is the major cause of maternal and child mortality in Ethiopia. This study aimed to explore the spatiotemporal distribution of short birth intervals in Ethiopia using data from four (2000, 2005, 2011, and 2016) consecutive demographic and health surveys.MethodsA total of 34,930 women were included in four consecutive Ethiopian Demographic and Health Surveys (EDHS). Thus, spatial autocorrelation, hotspot analysis, cluster analysis, and spatial interpolation were carried out for each survey separately to show the geographical and temporal pattern of at-risk areas for short birth intervals in Ethiopia. Finally, the highest proportion of short birth interval risk areas in each survey period was mapped. Geospatial analysis was conducted by using ArcGIS V.10.8 and R version 4.2.ResultsThe results of the study indicated that the overall proportion of short birth intervals of women in Ethiopia was highest in 2000 (47.5%), 2005 (46.4%), 2011 (44.7%), and the lowest in 2016 (44.0%). The values for Global Moran’s I (MI = 0.177665 p = 0.0016, MI = 0.2024, p = 0.001, MI = 0.10023, p = 0.002, and MI = 0.764, p = 0.008) showed that the presence of significant short birth interval clustering in Ethiopian administrative zones in 2000, 2005, 2011, and 2016, respectively. The hotspot areas for short birth intervals were consistently observed in the zones in the Somali Region and the zones in the Harari Region for all the EDHS years. In addition, the survival status of the index child, residence, breastfeeding practice, religion, and the spatial variable (Si) were significantly associated with the short birth interval of women in all the EDHS years.ConclusionSpatial distribution of short birth intervals differs across Ethiopian administrative zones. Survival status of the index child being dead, rural residential, and no breastfeeding practice are the risk factors for short birth intervals of women that increase the risk of a short birth interval among women in all the EDHS years. Therefore, the hotspot areas and indicators need interventions to decrease the short birth interval of women.
Does bimanual coordination training benefit inhibitory function in older adults?
Authors: Torre Marta Maria , Langeard Antoine , Alliou Louis , Temprado Jean-Jacques 
Published: April 2023

IntroductionWhether complex movement training benefits inhibitory functions and transfers the effects to non-practiced motor and cognitive tasks is still unknown. The present experiment addressed this issue using a bimanual coordination paradigm. The main hypothesis was that bimanual coordination training allows for improving the involved cognitive (i.e., inhibition) mechanisms and then, transferring to non-practiced cognitive and motor tasks, that share common processes.Methods17 older participants (72.1 ± 4.0 years) underwent 2 training and 3 test sessions (pre, post, and retention one week after) over three weeks. Training included maintaining bimanual coordination anti-phase pattern (AP) at high frequency while inhibiting the in-phase pattern (IP). During the test sessions, participants performed two bimanual coordination tasks and two cognitive tasks involving inhibition mechanisms. Transfer benefits of training on reaction time (RT), and total switching time (TST) were measured. In the cognitive tasks (i.e., the Colour Word Stroop Task (CWST) and the Motor and Perceptual Inhibition Test (MAPIT)), transfer effects were measured on response times and error rates. Repeated one-way measures ANOVAs and mediation analyses were conducted.ResultsResults confirmed that training was effective on the trained task and delayed the spontaneous transition frequency. Moreover, it transferred the benefits to untrained bimanual coordination and cognitive tasks that also involve inhibition functions. Mediation analyses confirmed that the improvement of inhibitory functions mediated the transfer of training in both the motor and cognitive tasks.DiscussionThis study confirmed that bimanual coordination practice can transfer training benefits to non-practiced cognitive and motor tasks since presumably they all share the same cognitive processes.
Corticosterone enhances formation of non-fear but not fear memory during infectious illness
Authors: Hill Alice , Johnston Colin , Agranoff Isaac , Gavade Swapnil , Spencer-Segal Joanna 
Published: April 2023

IntroductionSurvivors of critical illness are at high risk of developing post-traumatic stress disorder (PTSD) but administration of glucocorticoids during the illness can lower that risk. The mechanism is not known but may involve glucocorticoid modulation of hippocampal- and amygdala-dependent memory formation. In this study, we sought to determine whether glucocorticoids given during an acute illness influence the formation and persistence of fear and non-fear memories from the time of the illness.MethodsWe performed cecal ligation and puncture in male and female mice to induce an acute infectious illness. During the illness, mice were introduced to a neutral object in their home cage and separately underwent contextual fear conditioning. We then tested the persistence of object and fear memories after recovery.ResultsGlucocorticoid treatment enhanced object discrimination but did not alter the expression of contextual fear memory. During context re-exposure, neural activity was elevated in the dentate gyrus irrespective of fear conditioning.ConclusionsOur results suggest that glucocorticoids given during illness enhance hippocampal-dependent non-fear memory processes. This indicates that PTSD outcomes in critically ill patients may be improved by enhancing non-fear memories from the time of their illness.