bluuebunny/crossref_metadata_2025_split huggingface dataset:
School-Based Mindfulness Enhances Psychological Well-Being in Adolescents after Parental Divorce: A Systematic Review
Authors: Slametiningsih  , Achir Yani S Hamid  , Imami Nur Rachmah  , Mustikasari  , Raden Irawati Ismail  
Published: January 2025

Background: Adolescents experience various psychosocial challenges, including low self-esteem, depression, substance abuse, and risky behaviors, which adversely affect their psychological well-being. School-based mindfulness interventions have emerged as effective strategies for promoting positive mental health among adolescents. Objective: This systematic review evaluates the effectiveness of school-based mindfulness programs in enhancing psychological well-being in adolescents after parental divorce. Methods: Following PRISMA guidelines, the review analyzed studies identified from nine major databases—Cochrane, Clinical Key, BMC, Medrix, ProQuest, PubMed, Wiley, ScienceDirect, and Taylor & Francis—between February and May 2024. Included studies consisted of randomized controlled trials (RCTs) and quasi-experimental studies focusing on school-based interventions targeting adolescent mental health. The JBI Critical Appraisal Checklist was employed to assess the risk of bias. Results: Out of 757 screened articles, 10 studies involving 27,680 participants from countries including Belgium, Spain, Italy, and Poland were selected. Findings suggest that school-based mindfulness interventions significantly improve psychological well-being and reduce mental health symptoms. Conclusion: Mindfulness-based interventions, when integrated into school environments with consistent support from families and educators, effectively promote psychological well-being among adolescents and reduce mental health risks.
The Effectiveness of Audiovisual Media and Snakes and Ladders Games on Increasing Knowledge and Skills of Brushing Teeth in Early Childhood Jambi City
Authors: Willia Novita Eka Rini  , Adelina Fitri  , Vinna Rahayu Ningsih  
Published: January 2025

Background: Dental health is the most important part of general oral health, especially during a child's growth and development. Children with dental caries experience loss of chewing ability and problems with digestion, resulting in disruption of growth and development. Dental caries in children is still a fairly high prevalence problem in Indonesia. Aims: This research aimed to see the effect of audiovisual media to Increasing Knowledge and Skills of Brushing Teeth in Early Childhood. Methods: This study used a pretest-posttest control group design. The subjects of this study were PAUD students in Jambi City with a 1:1 ratio of respondents, where the experimental group was 40 students and the control group was 40 students. Primary data can be collected through various methods, such as surveys, interviews, direct observation, or scientific experiments. Data analysis employed the paired t-test. Results: The measurable test shows a p-value of 0.000 < 0.05. It indicates that the understanding of the students in the intervention group and the control group differs significantly. The analysis's findings indicate a significant difference between the students' skills in the intervention group and the control group, with a p-value of 0.000 <0.05. Conclusion: Audiovisual media and snake and ladders games are effective in increasing knowledge and skill of brushing teeth in early childhood.
Characterization of resistant starch (type III) produced from banana (Musa acuminata x balbisiana CV Awak) pseudostem
Authors: Ho Lee Hoon , Segar Rashneve , Mathihalagan Fairus Shalini , Zakaria Siti Husna , Tuan Mazlam Sharifah Amirah Husna 
Published: January 2025

Banana (Musa acuminata x balbisiana cv. Awak) contains minerals, vitamins, fiber, proteins, carbohydrates, and other vital nutrients. Unfortunately, the banana pseudostem are left as a waste after harvesting. It is rationale to process banana pseudostem into useful product such as functional food ingredients for the application of food industry. The present study aimed to determine the functional properties of resistant starch (type III) produced from banana pseudostem as well as to observe the morphology of resistant starch produced. The native starch from banana pseudostem was isolated and processed into resistant starch (type III) by autoclaving process. The characterization of resistant starch of banana pseudostem (RSBP) was compared with native banana starch and commercial starch (i.e., corn and potato starches) in terms of functional group, crystalline structure, paste clarity, pH, freeze thaw stability, particle size distribution, and morphology. The results showed that the hydroxy, alkene, carbonyl, and alkane groups were found presence in all the evaluated starches. However, the sulphate group was only existed in RSBP. Moreover, RSBP obtained a B-type crystalline structure. Native starch of banana pseudostem exhibited a smooth surface devoid of dents with a mixture of irregular granule shapes of oval, spheroid, and elongated granule shape. All the evaluated starches were categorized as acidic food ingredient. Potato starch exhibited the highest value for paste clarity and particle size distribution. RSBP had significant higher freeze thaw stability than other starches. Banana pseudostem can be considered a feasible low-cost raw material for production of resistant starch (type III) then, a suitable functional food can be developed, depending on the desired end-product quality.
No-Test Telehealth Medication Abortion Services Provided by US-Based Clinicians in 21 States and the District of Columbia, 2020‒2022
Authors: Godfrey Emily M. , Fiastro Anna E. , Thayer Erin K. , Gomperts Rebecca , Orlando Sophia M. , Myers Caitlin K. 
Published: January 2025

Objectives. To evaluate the association between distance from closest abortion facility and number of fulfilled requests through no-test telehealth medication abortion (NTMA) asynchronous service. Methods. Using deidentified 2020–2022 electronic medical record data from Aid Access users in US states where NTMA is prescribed by US-based clinicians, we describe individual user demographics and their resident county characteristics. We conducted a county-level geospatial analysis of distance to abortion facility (Myers Abortion Facility Database) on fulfilled requests using Poisson regression. Results. US-based clinicians fulfilled NTMA requests to 8411 individuals in 21 states and the District of Columbia. Each 100-mile increase in distance to an abortion facility increased per-capita NTMA by 61% (95% confidence interval [CI] = 26%, 86%). Most individuals were aged 20 to 29 years (54%), had no living children (57%), were less than 6 weeks’ gestation (62%), and lived in urban areas (65%). Almost half (49%) lived in higher socially vulnerable counties compared with 17% in less socially vulnerable counties. Conclusions. In the United States, telehealth medication abortion is a critically important service for individuals who are young, socially vulnerable, and living in counties far from abortion care facilities. Public Health Implications. With abortion now banned or highly restricted in 22 US states, telehealth abortion services are necessary to maintain essential reproductive health services. ( Am J Public Health. 2025;115(2):221–231. https://doi.org/10.2105/AJPH.2024.307892 )
Cloning, Expression, Purification and Characterisation of Glutamate Decarboxylase (Rv3432c) from Mycobacterium tuberculosis
Authors: Rai Rupal , Paroha Ruchi , Rai Sandesh , Singh Anirudh K. , Chaurasia Rashmi , Agarwal Nisheeth , Pandey Megha Katare , Chaurasiya Shivendra K. 
Published: January 2025

Abstract

Glutamate decarboxylase (Gad), a pyridoxal 5'-phosphate (PLP)-dependent enzyme, catalyses the conversion of glutamate to γ-aminobutyric acid (GABA), consuming a proton in the process and contributing to intracellular pH homeostasis in bacteria. The presence and function of the Gad-dependent mechanism in mycobacteria remain largely unexplored. Our study aimed to characterize Gad activity in Mycobacterium tuberculosis (Mtb). We detected Gad enzymatic activity and protein expression in mycobacterial cells, observing activity in live cells of both Mtb and Mycobacterium smegmatis (MS). Gad activity and GABA production were also detected in cell lysates of Mtb and MS. The gadB gene from Mtb was cloned, expressed, and purified under native conditions using MS as an expression host. Initial attempts to express Gad in Escherichia coli (E. coli) resulted in the protein being insoluble and inactive when purified under denaturing conditions. Therefore, an acetamide-inducible expression system was used for overexpression in MS. Recombinant GadB was purified using immobilized metal affinity chromatography, and its molecular weight was determined to be 51.2 kDa via SDS-PAGE. The purified GadB enzyme was active at both neutral and acidic pH. Its activity was found to be PLP-dependent, with optimal activity at pH 7.2 and 50°C. These findings suggest that Gad is expressed in Mtb at both normal as well as acidic pH, indicating the possible existence of a Gad-dependent acid resistance mechanism in Mtb.
Structure-guided optimization of small molecules targeting the yeast casein kinase, Yck2, as a therapeutic strategy to combat Candida albicans
Authors: Cowen Leah , Puumala Emily , Nandakumar Meganathan , Yiu Bonnie , Stogios Peter , Strickland Benjamin , Zarnowski Robert , Wang Xiaoyu , Williams Noelle , Savchenko Alexei , Andes David , Robbins Nicole , Whitesell Luke , Willson Timothy 
Published: January 2025

Abstract

Candida albicans is the most common cause of life-threatening fungal infection in the developed world but remains a therapeutic challenge. Protein kinases have been rewarding drug targets across diverse indications but remain untapped for antifungal development. Previously, screening kinase inhibitors against C. albicans revealed a 2,3-aryl-pyrazolopyridine, GW461484A (GW), which targets casein kinase 1 (CK1) family member Yck2. Here, we report optimization of GW via two complementary approaches, synthesis of bioisosteres possessing an imidazo[1,2-a]pyridine core, and R-group substitution of GW’s pyrazolo[1,5-a]pyridine core. Characterization of compounds synthesized revealed two 6-cyano derivatives with improved pharmacological properties that retained whole-cell bioactivity and selectivity for fungal Yck2 compared to human CK1α. Efficacy studies in mice indicated both analogs possess single-agent activity against C. albicans resistant to first-line echinocandin antifungals and potentiate non-curative echinocandin treatment. Results validate Yck2 as an antifungal target and encourage further development of inhibitors acting by this previously unexploited mode of action.
Does SARS-CoV-2 Infection Increase Risk of Neuropsychiatric and Related Conditions? Findings from Difference-in-Differences Analyses
Authors: Chen Yong , Lu Yiwen , Tong Jiayi , Zhang Dazheng , Chen Jiajie , Li Lu , Lei Yuqing , Zhou Ting , Aragon Leyna , Becich Michael , Blecker Saul , Blum Nathan , Christakis Dimitri , Hornig Mady , Hornig-Rohan Maxwell , Jhaveri Ravi , Jones William , Keebler Amber , Kelleher Kelly , Kim Susan , Mosa Abu , Pajer Kathleen , Platt Jonathan , Schwenk Hayden , Taylor Bradley , Utidjian Levon , Williams David , Prasad Raghuram , Elia Josephine , Forrest Christopher 
Published: January 2025

Abstract

The COVID-19 pandemic has been associated with increased neuropsychiatric conditions in children and youths, with evidence suggesting that SARS-CoV-2 infection may contribute additional risks beyond pandemic stressors. This study aimed to assess the full spectrum of neuropsychiatric conditions in COVID-19 positive children (ages 5–12) and youths (ages 12–20) compared to a matched COVID-19 negative cohort, accounting for factors influencing infection risk. Using EHR data from 25 institutions in the RECOVER program, we conducted a retrospective analysis of 326,074 COVID-19 positive and 887,314 negative participants matched for risk factors and stratified by age. Neuropsychiatric outcomes were examined 28 to 179 days post-infection or negative test between March 2020 and December 2022. SARS-CoV-2 positivity was confirmed via PCR, serology, or antigen tests, while negativity required negative test results and no related diagnoses. Risk differences revealed higher frequencies of neuropsychiatric conditions in the COVID-19 positive cohort. Children faced increased risks for anxiety, OCD, ADHD, autism, and other conditions, while youths exhibited elevated risks for anxiety, suicidality, depression, and related symptoms. These findings highlight SARS-CoV-2 infection as a potential contributor to neuropsychiatric risks, emphasizing the importance of research into tailored treatments and preventive strategies for affected individuals.
Lassa Fever Survivors: Long-Term Health Effects and Chronic Sequelae – A Scoping Review
Authors: Saka Sulymon A. , Lawal Qudus O. , Otaigbe Osahon , Blackie Faustina F. , Ighodaro Osazuwa , Odafen Perpetua I. , Okogbenin Sylvanus 
Published: January 2025

Abstract

This scoping review explores the long-term health effects and chronic sequelae among Lassa fever survivors, highlighting significant physical, psychological, and functional challenges. Hearing loss, a prominent sequela, often coexists with neurological deficits, such as balance difficulties and cognitive dysfunction, impacting survivors' quality of life. Persistent renal, ophthalmic, and psychiatric complications, including depression and delirium, further underscore the multifaceted consequences of the disease. Despite the critical nature of these findings, research gaps remain, particularly the lack of longitudinal studies and limited geographic scope beyond Sierra Leone and Nigeria. These gaps hinder a comprehensive understanding of the disease's long-term impact. Addressing these issues through targeted interventions and expanded research is essential for improving post-recovery care and outcomes for Lassa fever survivors.
Deep analysis of classroom teaching behavior from the perspective of artificial intelligence: Centered on effective questioning models and exploration of "Four How" questions
Authors: Ou Qizhong , Wu Songqiao , Chen Xinglin 
Published: January 2025

Abstract

Classroom questioning is a critical element of teaching, facilitating interaction between teachers and students.Effective questioning plays a pivotal role in enhancing students’ cognitive abilities, sparking interest in learning, and supporting knowledge retention. This study analyzes recorded exemplary classroom videos using an intelligent classroom data analysis cloud platform, integrating an effective questioning model and the "Four How" theory. The analysis is based on five dimensions: teacher questioning type, student response method, student response type, teacher response attitude, and the "Four How" questions. The findings reveal that while teachers often respond positively to students, their questions tend to focus on "what" inquiries.Furthermore,students frequently provide mechanical or memory-based responses. In light of these findings,the study recommends optimizing question design to foster higher-order thinking, improving questioning techniques to enhance teacher-student interaction, strengthening student guidance for deeper engagement, and refining feedback to cultivate problem awareness. This research provides valuable data-driven insights into teaching behaviors and offers strategies for enhancing classroom teaching quality in schools.
Risk Assessment and Source Analysis of Heavy Metals in Soil around Nansihu Lake Based on APCS-MLR and PMF Models
Authors: Yan Beibei , Lv Xiaofang , Wang Tao , Wang Min , Zhang Ruilin , song chengyuan 
Published: January 2025

Abstract

Heavy metal pollution in the Nansihu area was evaluated using the geo-accumulation index, pollution load index, and potential ecological risk assessment, with source analysis performed through the absolute principal component-multiple linear regression (APCS-MLR) model and positive definite matrix factorization (PMF) model. The average concentrations of As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn were below standardized background values; however, Hg exhibited significant enrichment, with an average concentration 1.37 times higher than the Jining City soil background, indicating localized point-source pollution driven by anthropogenic activities. Source analysis identified four major contributors: agricultural, natural, transportation, and industrial sources. The APCS-MLR model attributed 34.63%, 29.17%, 25.81%, and 10.39% of the pollution to agricultural, natural, transportation, and industrial sources, respectively, while the PMF model assigned 22.93%, 30.46%, 25.13%, and 21.48%. These results highlight the distinct roles of human activities in heavy metal contamination, particularly Hg, and demonstrate the value of integrating APCS-MLR and PMF models for comprehensive source apportionment and environmental management strategies.
A Case of Advanced Peripheral Artery Disease with Advanced Coronary Artery Disease- A case report
Authors: Aftab Unaiza , Duseja Nikhil 
Published: January 2025

Abstract

Objective: This case report describes a rare presentation of triple-vessel coronary artery disease (3 CAD) co-existing with advanced peripheral arterial disease (PAD) in a 65-year-old patient, focusing on the challenges in diagnosis and management.Methods: A 65-year-old patient presented with retrosternal chest pain. A thorough clinical examination, electrocardiography (ECG), and additional diagnostic tests revealed STEMI. Coronary angiography confirmed advanced PAD alongside triple-vessel coronary artery disease (CAD). The patient was advised to undergo coronary artery bypass grafting (CABG), and a full-body aortogram was conducted to further assess the extent of PAD.Results: Significant blockages were identified in major peripheral arteries, including the right brachial artery and both femoral arteries, along with three major coronary arteries: the right coronary artery (RCA), left anterior descending artery (LAD), and left circumflex artery (LCX).Conclusion: This case highlights the importance of a multidisciplinary approach in managing complex cases involving both advanced CAD and advanced PAD. Early and coordinated interventions can significantly improve patient outcomes in these high-risk situations.
Male Breast Cancer and BRCA2: A Study from West Bengal, India
Authors: Roy Abhishikta Ghosh , Singh Vanya , Sarkar Biswanath , BANDYOPADHYAY ARUP Ratan 
Published: January 2025

Abstract

One of the known risk factors for male breast cancer is germline mutations in the BRCA2 gene, which are linked to genomic alterations. Men carrying a germline BRCA2 mutation have a higher risk of developing breast carcinoma than the general population. Breast Cancer is the fifth leading cause of cancer-related deaths worldwide and the most common cancer in developed and developing countries. Male breast cancer accounts for approximately 1% of all breast cancer cases. The incidence of male breast cancer has increased over the past few decades, yet its etiology is still poorly understood, which may be due to the rarity of the disease and the lack of large-scale genetic epidemiologic studies. The present study aimed to evaluate the risk of developing Breast Carcinoma among Bengalee Hindu males. For the study, 36 male patients and 70 matched controls were included in this study. DNA sequencing and gene expression analysis revealed a significant association (p < 0.0001) between male breast cancer patients and BRCA2 mutations (rs1799943). There were many intronic variations whose associations were not determined. A 2.4-fold downregulation of tumour proteins was associated with patient risk, whereas controls showed a 1.28-fold upregulation. The present study, being the first attempt in Eastern India, envisaged a significant association (p < 0.001) of the disease for mutations in the exonic and flanking regions of the gene (OR-9.92; 95% CI = 4.40-22.37; p < 0.0001).
High-Accuracy De Novo Prediction for N- and O-linked Glycopeptides Across Multiple Fragmentation Techniques
Authors: Zhang Qianqiu , Mao Zeping , Chen Yuling , Shan Baozhen , Deng Haiteng , Li Ming 
Published: January 2025

Abstract

N- and O-glycosylation, the most intricate post-translational modifications of proteins, is essential for regulating biological functions. Glycoproteomics faces substantial challenges, particularly in the analysis of O-glycans due to its diversity compared to N-glycans. Additionally, tandem mass spectrometry data patterns exhibit variability among different fragmentation methods, such as stepped collision energy higher-energy collisional dissociation (sceHCD) and electron transfer higher-energy collisional dissociation (EThcD). Existing algorithms often lack sensitivity and are limited to sceHCD fragmentation, restricting their practical application. To address these limitations, we introduce GlycopepECHO, the first deep-learning-based de novo glycopeptide algorithm that captures correlations between spectrum and glycopeptide fragmentation ions among N- and O-glycans. GlycopepECHO achieves over 92% glycan recall and around 95% glycan precision on N-glycan fragmented by both sceHCD and EThcD. It additionally allows O-glycan de novo sequencing benefits from zero-shot learning. GlycopepECHO expands the analytical capabilities of glycoproteomics, shedding light on the diverse roles of glycosylation.
Effect of Fatty Acyl Composition for Lysophosphatidylinositol on Neuroinflammatory Responses in Primary Neuronal Cultures
Authors: Brenneman Douglas E. , Petkanas Dean , Ippolito Michael , Ward Sara Jane 
Published: January 2025

Abstract

Lysophosphatidylinositol (LPI) is an endogenous signaling molecule for the GPR55 receptor. Previous studies have shown that arachidonoyl-lysophosphatidylinositol (LPI-20:4) produced an increase in the inflammatory mediators NLPR3 (inflammasome − 3 marker) and IL-1b in neurons from both rat dorsal root ganglion (DRG) and hippocampal cultures. Because LPI is comprised of a family of lipid structures that vary in fatty acyl composition, the current work examined neuroinflammatory responses to various LPI structures in DRG and hippocampal cultures as assessed by high content fluorescent imaging. Major endogenous LPI fatty acyl structures consisting of 16:0, 18:0, 18:1 or 20:4 were compared for their effects on IL-1b, NLRP3 and GPR55 immunoreactive areas of neurites and cell bodies after a 6-hour treatment. Among these four LPI structures, only LPI-20:4 treatment produced increases in immunoreactive areas for GPR55, NLRP3 and IL-1b in DRG and hippocampal neurites. In contrast, all other LPI structures tested produced a decrease in all of these inflammatory immunoreactive areas in both neurites and cell bodies. Additional studies with LPI-20:4 treatment indicated that IL-6, IL-18 and TNF-a were significantly increased in neurites of DRG and hippocampal cultures. However, oleoyl-lysophosphatidylinositol (LPI-18:1) treatment produced decreases in these three cytokines. Using the viability dye alamar blue, LPI-20:4 was shown to produce concentration-dependent decreases, whereas all other endogenous LPI structures produced increases with this assay. These studies indicate that fatty acyl structure is the major determinant of LPI for neuroinflammatory responses in DRG and hippocampal cultures, with LPI-20:4 showing pro-inflammatory effects and all other endogenous LPIs tested exhibited anti-inflammatory responses.