bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the structure, function of thiamine pyrophosphate riboswitch, and designing small molecules for antibacterial activity
Authors: Wakchaure Padmaja D. , Ganguly Bishwajit 
Published: January 2023

AbstractDuring the last decade, riboswitches emerged as new small‐molecule sensing RNA in bacteria. Thiamine pyrophosphate (TPP) riboswitch is widely distributed and occurs in plants, bacteria, fungi, and archaea. Extensive biochemical, structural, and genetic studies have been carried out to elucidate the recognition mechanism of TPP riboswitches. However, a comprehensive report summarizing all information on recognition principles and newly designed ligands for TPP riboswitch is scarce in the literature. This review gives a comprehensive understanding of the TPP riboswitch's structure, mechanism, and methods applied to design ligands for the TPP riboswitch. The ligand‐bound TPP riboswitch was studied with various experimental and theoretical techniques to elucidate the conformational dynamics. The mutation studies shed light on the significance of pyrimidine sensing helix for the binding of ligands. Further, the structure–activity relationship study and fragment‐based approach lead to the development of ligands with Kd values at the sub‐micromolar level. However, there is a need to design more potent inhibitors for TPP riboswitch for therapeutic applications. The recent advancements in ligand design highlight the TPP riboswitch as a promising target for developing new antibiotics.This article is categorized under:
Regulatory RNAs/RNAi/Riboswitches > Riboswitches
Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs
Preservers of Triple Transition Pseudo-Probabilities in Connection with Orthogonality Preservers and Surjective Isometries
Authors: Peralta Antonio M. 
Published: January 2023

AbstractWe prove that every bijection preserving triple transition pseudo-probabilities between the sets of minimal tripotents of two atomic JBW$$^*$$∗-triples automatically preserves orthogonality in both directions. Consequently, each bijection preserving triple transition pseudo-probabilities between the sets of minimal tripotents of two atomic JBW$$^*$$∗-triples is precisely the restriction of a (complex-)linear triple isomorphism between the corresponding JBW$$^*$$∗-triples. This result can be regarded as triple version of the celebrated Wigner theorem for Wigner symmetries on the posets of minimal projections inB(H). We also present a Tingley type theorem by proving that every surjective isometry between the sets of minimal tripotents in two atomic JBW$$^*$$∗-triples admits an extension to a real linear surjective isometry between these two JBW$$^*$$∗-triples. We also show that the class of surjective isometries between the sets of minimal tripotents in two atomic JBW$$^*$$∗-triples is, in general, strictly wider than the set of bijections preserving triple transition pseudo-probabilities.
Das Shishaphänomen: Gefahren einer unterschätzten Droge
Authors: Rabenhorst D. , Mandilaras G. , Haas N. , Kuhlee I. 
Published: January 2023

ZusammenfassungDer Konsum von Wasserpfeifen hat in den letzten Jahren v. a. bei Jugendlichen deutlich zugenommen. Die Risiken und Gefahren werden nicht nur bei den Konsumenten unterschätzt. Neben einer besseren Aufklärung der potenziellen Nutzergruppe sind für die im Gesundheitswesen Tätigen eine Sensibilisierung für mögliche Komplikationen und eine genaue Kenntnis des Vorgehens bei möglicher Intoxikation durch Kohlenmonoxid nach Shishagebrauch unerlässlich.Eine unklare Bewusstseinsstörung ist ein häufiger Einlieferungsgrund in die Notaufnahme. Durch gezielte Anamnese und genauen Blick auf die Blutgasanalyse konnte bei einem jugendlichen Patienten eine Kohlenstoffmonoxidintoxikation nach Shishakonsum als Ursache gefunden werden. Unter intensivmedizinischer Überwachung und Sauerstoffgabe verlief dieser Fall komplikationslos.
Final efficacy and safety results and biomarker analysis of a phase 2 study of cabozantinib in Japanese patients with advanced renal cell carcinoma
Authors: Nakaigawa Noboru , Tomita Yoshihiko , Tamada Satoshi , Tatsugami Katsunori , Osawa Takahiro , Oya Mototsugu , Kanayama Hiroomi , Miura Yuji , Sassa Naoto , Nishimura Kazuo , Nozawa Masahiro , Masumori Naoya , Miyoshi Yasuhide , Kuroda Shingo , Kimura Akiko 
Published: January 2023

Abstract
Background
Cabozantinib was established as the standard of care for the treatment of patients with renal cell carcinoma (RCC) whose disease had progressed after vascular endothelial growth factor receptor tyrosine kinase inhibitor (VEGFR-TKI) therapy in the global randomized trial METEOR. A phase 2 study was conducted to bridge the findings in METEOR to Japanese patients. Here, we report a biomarker analysis and update the efficacy and safety results of cabozantinib treatment.

Methods
Japanese patients with RCC who received at least one prior VEGFR-TKI were enrolled and received cabozantinib 60 mg orally once daily. The primary endpoint was objective response rate. Secondary endpoints included progression-free survival, overall survival, and safety. Exploratory analyses included the relationship between plasma protein hepatocyte growth factor (HGF) levels and treatment responses.

Results
In total, 35 patients were enrolled. The median treatment duration was 58.3 (range 5.1–131.4) weeks. The objective response rate was 25.7% (90% confidence interval [CI] 14.1–40.6). Kaplan–Meier estimate of median progression-free survival was 11.1 months (95% CI 7.4–18.4). The estimated progression-free survival proportion was 73.1% (95% CI 54.6–85.0) at 6 months. Median overall survival was not reached. Adverse events were consistent with those in METEOR and the safety profile was acceptable. Nonresponders to cabozantinib showed relatively higher HGF levels than responders at baseline.

Conclusions
Updated analyses demonstrate the long-term efficacy and safety of cabozantinib in Japanese patients with advanced RCC after at least one VEGFR-TKI therapy. Responders tended to show lower baseline HGF levels ClinicalTrials.gov Identifier: NCT03339219.
Spotted cucumber beetle (Diabrotica undecimpunctata howardi) is attracted to vittatalactone, the pheromone of striped cucumber beetle (Acalymma vittatum)
Authors: Haber Ariela I. , Pasteur Kayla , Guzman Filadelfo , Boyle Sean M. , Kuhar Thomas P. , Weber Donald C. 
Published: January 2023

AbstractSpotted cucumber beetle (Diabrotica undecimpunctata howardi; also known as southern corn rootworm) is a pest feeding on many crops, including cucurbits, corn (maize), peanuts, beans, and other crops and wild plants in North America. On cucurbit crops, where it co-occurs with striped cucumber beetle (Acalymma vittatum), both are key pests that vector Erwinia tracheiphila, a destructive pathogen that causes bacterial wilt. Here we deployed synthetic vittatalactone, the male-produced aggregation pheromone of A. vittatum, as lures in the field, to measure attraction of the target and non-target insects. Both sexes of D. u. howardi were strongly attracted to the vittatalactone lures (5–7.5-fold compared to blank trap) throughout the growing season in Maryland and Virginia, USA. Moreover, D. u. howardi was more attracted to live A. vittatum males feeding on squash plants than to plants with females or without any beetles, confirming the genuine organismal cross-attraction between these two key pests. The kairomonal attraction to vittatalactone suggests that it functions as a “keystone semiochemical,” mediating the colonization of cucurbits by multiple herbivore species. Such cross attraction could allow for use of vittatalactone in management of multiple pest species, with potential reduction of pesticide use in cucurbit crops.
Landslide monitoring techniques in the Geological Surveys of Europe
Authors: Auflič Mateja Jemec , Herrera Gerardo , Mateos Rosa María , Poyiadji Eleftheria , Quental Lídia , Severine Bernardie , Peternel Tina , Podolszki Laszlo , Calcaterra Stefano , Kociu Arben , Warmuz Bartłomiej , Jelének Jan , Hadjicharalambous Kleopas , Becher Gustaf Peterson , Dashwood Claire , Ondrus Peter , Minkevičius Vytautas , Todorović Saša , Møller Jens Jørgen , Marturia Jordi 
Published: January 2023

AbstractLandslide monitoring is a mandatory step in landslide risk assessment. It requires collecting data on landslide conditions (e.g., areal extent, landslide kinematics, surface topography, hydrogeometeorological parameters, and failure surfaces) from different time periods and at different scales, from site-specific to local, regional, and national, to assess landslide activity. In this analysis, we collected information on landslide monitoring techniques from 17 members of the Earth Observation and Geohazards Expert Group (from EuroGeoSurveys) deployed between 2005 and 2021. We examined the types of the 75 recorded landslides, the landslide techniques, spatial resolution, temporal resolution, status of the technique (operational, non-operational), time of using (before the event, during the event, after the event), and the applicability of the technique in early warning systems. The research does not indicate the accuracy of each technique but, rather, the extent to which Geological Surveys conduct landslide monitoring and the predominant techniques used. Among the types of landslides, earth slides predominate and are mostly monitored by geological and engineering geological mapping. The results showed that Geological Surveys mostly utilized more traditional monitoring techniques since they have a broad mandate to collect geological data. In addition, this paper provides new insights into the role of the Geological Surveys on landslide monitoring in Europe and contributes to landslide risk reduction initiatives and commitments (e.g., the Kyoto Landslide Commitment 2020).
The Swedish medical birth register during five decades: documentation of the content and quality of the register
Authors: Cnattingius Sven , Källén Karin , Sandström Anna , Rydberg Henny , Månsson Helena , Stephansson Olof , Frisell Thomas , Ludvigsson Jonas F. 
Published: January 2023

AbstractPregnancy-related factors are important for short- and long-term health in mothers and offspring. The nationwide population-based Swedish Medical Birth Register (MBR) was established in 1973. The present study describes the content and quality of the MBR, using original MBR data, Swedish-language and international publications based on the MBR.The MBR includes around 98% of all births in Sweden. From 1982 onwards, the MBR is based on prospectively recorded information in standardized antenatal, obstetric, and neonatal records. When the mother and infant are discharged from hospital, this information is forwarded to the MBR, which is updated annually. Maternal data include information from first antenatal visit on self-reported obstetric history, infertility, diseases, medication use, cohabitation status, smoking and snuff use, self-reported height and measured weight, allowing calculation of body mass index. Birth and neonatal data include date and time of birth, mode of delivery, singleton or multiple birth, gestational age, stillbirth, birth weight, birth length, head circumference, infant sex, Apgar scores, and maternal and infant diagnoses/procedures, including neonatal care. The overall quality of the MBR is very high, owing to the semi-automated data extraction from the standardized regional electronic health records, Sweden’s universal access to antenatal care, and the possibility to compare mothers and offspring to the Total Population Register in order to identify missing records. Through the unique personal identity numbers of mothers and live-born offspring, the MBR can be linked to other health registers. The Swedish MBR contains high-quality pregnancy-related information on more than 5 million births during five decades.
Celsr2 Knockout Alleviates Inhibitory Synaptic Stripping and Benefits Motoneuron Survival and Axon Regeneration After Branchial Plexus Avulsion
Authors: Yu Lingtai , Liu Mengfan , Li Fuxiang , Wang Qianghua , Wang Meizhi , So Kwok-Fai , Qu Yibo , Zhou Libing 
Published: January 2023

Abstract Axotomy-induced synaptic stripping modulates survival and axon regeneration of injured motoneurons. Celsr2 is supposed to mediate homophilic interactions of neighboring cells during development, and its role in synaptic stripping remains unknow. In a model of brachial plexus avulsion, Celsr2 knockout improved functional recovery, motoneuron survival, and axon regeneration. Celsr2 was indicated to express in spinal motoneurons, excitatory and inhibitory interneurons, astrocytes, and a subset of oligodendrocytes using Celsr2LacZ mice. Double immunostaining showed that the coverage of inhibitory and excitatory vesicles on injured motoneurons were remarkably reduced after injury, whereas more inhibitory vesicles were maintained in Celsr2−/− mutants than control mice. In the ultrastructure, the density of inhibitory F-boutons on injured motoneurons was higher in Celsr2−/− mutants than controls. Conditional knockout of Celsr2 in astrocytes or oligodendrocytes showed the similar axotomy-induced synaptic withdrawal to the control. RNAseq of injured spinal samples identified 12 MHC I molecules with significant changes between Celsr2−/− and control mice. After injury, expression of MHC I surrounding injured motoneurons was increased, particularly high in Celsr2−/− mutants. In conclusion, Celsr2 knockout enhances MHC I signaling, alleviates inhibitory synaptic stripping cell-autonomously, and contributes to motoneuron survival and regeneration, and Celsr2 is a potential target for neural repair.
Operational planning for public holidays in grocery retailing - managing the grocery retail rush
Authors: Obermair Elisabeth , Holzapfel Andreas , Kuhn Heinrich 
Published: January 2023

AbstractPublic holiday weeks cause specific challenges in grocery retailing as sales are raising and working days for logistics processes are reduced. The paper analyzes the operational planning challenges and solutions for demand planning and disposition as well as for warehouse and transportation management of grocery retailers in public holiday seasons. A total of 22 top managers representing 20 sales lines of 17 of the top 30 grocery retailers in Germany participated in the study. Semi-structured, face-to-face interviews with logistics managers were conducted and analyzed. Uncertainties and missing resources can be identified as the two main challenges of public holiday seasons in grocery retailing. Retailers implement numerous measures that can be summarized in three categories, i.e., the adjustment of workload profiles, the adaptation of resources and modifying processes. Literature has so far considered public holidays only to a limited extent, e.g., as a parameter in forecasting models or for the application of marketing instruments. This study is the first developing a framework and providing insights into operational planning in grocery retailing.
High GLI-1 Expression is a Reliable Indicator of Bad Prognosis in Newly Diagnosed Acute Leukemia Patients
Authors: El Zaiat Reham S. , Nabil Reem , Khalifa Khaled A. , El Feshawy Aliaa A. 
Published: January 2023

AbstractPurpose: To explore the expression and prognostic significance of Hedgehog signaling transcription factor GLI-1 in newly diagnosed acute myeloid leukemia (AML) patients. Methods: Clinical specimens were obtained from 46 recently diagnosed AML patients. Real-time qPCR was used to measure the GLI-1 mRNA expression in bone marrow mononuclear cells.Also, the relationship between GLI-1 mRNA levels and clinical variables and prognostic variables was assessed. Results: GLI-1 was overexpressed in the bone marrow samples of our patients. GLI-1mRNA expression did not differ significantly across different age groups, between both sexes, or between different FAB subtypes (P = 0.882, P = 0.246, and P = 0.890, respectively). GLI-1 expression varied significantly in different risk categories, with the greatest levels observed in 11 patients with poor risk (24.6 versus 22.7) compared to intermediate risk (5.2 versus 3.9; P = 0.006) and favorable risk (4.2 versus 3; P = 0.001). Comparing patients with the wild FLT3 allele to those with the mutant one, GLI-1 gene levels were considerably greater in those with the mutant allele of FLT3.Following induction chemotherapy, the levels of GLI-1 mRNA were significantly higher in 22 patients who did not experience complete remission (CR) diagnosed with de novo non-acute promyelocytic leukemia (APL) compared to 17 patients who did (P = 0.017). Significantly greater levels of expression were observed in each category of the patients with favorable risk; wild FLT3 allele (P = 0.033) and CR failure P = 0.005). Conclusion: GLI-1 overexpression is a risk factor for poor prognosis and could be a novel therapeutic target for AML.
A numerical study on the effects of DP steel microstructure on the yield locus and the stress–strain response under strain path change
Authors: Saai Afaf , Hopperstad Odd Sture , Fritz Jenny , Larsson Joachim 
Published: January 2023

Abstract
The mechanical response of dual phase (DP) steel exhibits a complex dependence on the microstructure. The chemical composition and microstructure characteristics of the phases have significant effects on the contrast between the response of the phases, which affects, not only the strength and ductility, but also the anisotropic response of DP steel under strain path changes. In this work, extended dislocation-based models of the ferrite and martensite phases of DP steel are proposed and used in a finite element based representative volume element approach to account for the contrast between the local response of the phases. The flow stress of each phase is computed as a function of the amount of substitutional and interstitial solute elements and the microstructural characteristics of the phase. Particular attention is paid to the phase model of the martensite phase. The model parameter controlling the storage of dislocations is related to the carbon content, which appears to be the most important parameter affecting the strength of martensite and its contrast with the local response of the ferrite phase. The model predicts a significant effect of the contrast between the local responses of the phases and the microstructure characteristics of each phase on the yield locus after prestraining and on the stress–strain behaviour after strain path change, i.e., forward-reverse shear loading and cyclic uniaxial tension–compression loading.
Patient Understanding of Chemotherapy and Goals of Care as Provided by Different Care Team Members
Authors: Suhail Shanzay , Basu Sandeep , Batoo Sameer A. , Al-Hattab Eyad S. , Kanj Ahmed Ola , Eidahl Barbara J. , Okuno Scott H. 
Published: January 2023

Abstract

Chemotherapy can be challenging and overwhelming for patients, but when patients are knowledgeable about chemotherapy, their comfort level, overall satisfaction, and coping improve. It is currently unknown whether patients prefer information about chemotherapy to be provided by specific care team members and whether demographic characteristics affect learning preferences. We developed a 31-question questionnaire that asked patients when chemotherapy information was discussed and who they wanted that information to come from. The questionnaire was given to 50 patients who had completed 1 cycle of chemotherapy. Patients were evenly distributed among age categories of 45 to 64 years, 65 to 74 years, and 75 years or older. Thirty participants (60%) were women, 33 (66%) had high school degrees, and 23 (46%) were receiving their first chemotherapy regimen. Sixty percent of patients best understood goals of care from oncologists, 70% wanted goals of care to come from oncologists, and 61% best understood and wanted to understand logistics of chemotherapy from oncologists. Sixty-six percent of patients understood adverse effects when they were explained by nursing staff, and 56% wanted explanations of adverse effects to come from nursing staff. Patients did not prefer a specific care team member or information source when receiving financial cost information. Patients often preferred to receive chemotherapy information from their oncologist; however, other members of the care team also provided information to patients in a way that was understood.
Protamine 1 as a secreted colorectal cancer-specific antigen facilitating G1/S phase transition under nutrient stress conditions
Authors: Ren Shengnan , Yang Dingquan , Dong Yongli , Ni Weidong , Wang Meiqi , Xing Lei , Liu Tong , Hou Wenjia , Sun Weixuan , Zhang Haolong , Yu Zhentao , Liu Yi , Cao Jingrui , Yan Hongbo , Feng Ye , Fang Xuedong , Wang Quan , Chen Fangfang 
Published: January 2023

Abstract
Purpose
Cancer testis antigens (CTAs) are optimal tumor diagnostic markers and involved in carcinogenesis. However, colorectal cancer (CRC) related CTAs are less reported with impressive diagnostic capability or relevance with tumor metabolism rewiring. Herein, we demonstrated CRC-related CTA, Protamine 1 (PRM1), as a promising diagnostic marker and involved in regulation of cellular growth under nutrient deficiency.

Methods
Transcriptomics of five paired CRC tissues was used to screen CRC-related CTAs. Capability of PRM1 to distinguish CRC was studied by detection of clinical samples through enzyme linked immunosorbent assay (ELISA). Cellular functions were investigated in CRC cell lines through in vivo and in vitro assays.

Results
By RNA-seq and detection in 824 clinical samples from two centers, PRM1 expression were upregulated in CRC tissues and patients` serum. Serum PRM1 showed impressive accuracy to diagnose CRC from healthy controls and benign gastrointestinal disease patients, particularly more sensitive for early-staged CRC. Furthermore, we reported that when cells were cultured in serum-reduced medium, PRM1 secretion was upregulated, and secreted PRM1 promoted CRC growth in culture and in mice. Additionally, G1/S phase transition of CRC cells was facilitated by PRM1 protein supplementation and overexpression via activation of PI3K/AKT/mTOR pathway in serum deficient medium.

Conclusions
In general, our research presented PRM1 as a specific CRC antigen and illustrated the importance of PRM1 in CRC metabolism rewiring. The new vulnerability of CRC cells was also provided with the potential to be targeted in future.

Graphical abstract
Diagnostic value and grow factor-like biofunction of PRM1 A represents the secretion process of PRM1 regulated by nutrient deficiency. B represents activation of PI3K/AKT/mTOR pathway of secreted PRM1.