bluuebunny/crossref_metadata_2025_split huggingface dataset:
Curcumin intake during lactation suppresses oxidative stress through upregulation of nuclear factor erythroid 2‐related factor 2 in the kidneys of fructose‐loaded female rat offspring exposed to maternal protein restriction
Authors: Yamaoka Shin , Sasaki Kotomi , Sato Shin 
Published: February 2023

AbstractBackgroundA high‐fructose diet causes the progression of chronic kidney disease. Maternal malnutrition during pregnancy and lactation increases oxidative stress, leading to chronic renal diseases later in life. We investigated whether curcumin intake during lactation could suppress oxidative stress and regulate NF‐E2‐related factor 2 (Nrf2) expression in the kidneys of fructose‐loaded female rat offspring exposed to maternal protein restriction.MethodsPregnant Wistar rats received diets containing 20% (NP) or 8% (LP) casein and 0 or 2.5 g “highly absorptive curcumin” /kg diet containing‐LP diets (LP/LP or LP/Cur) during lactation. At weaning, female offspring received either distilled water (W) or 10% fructose solution (Fr) and were divided into four groups: NP/NP/W, LP/LP/W, LP/LP/Fr, and LP/Cur/Fr. At week 13, glucose (Glc), triacylglycerol (Tg), and malondialdehyde (MDA) levels in the plasma, macrophages number, fibrotic area, glutathione (GSH) levels, glutathione peroxidase (GPx) activity, protein expression levels of Nrf2, heme oxygenase‐1 (HO‐1), and superoxide dismutase 1 (SOD1) in the kidneys were examined.ResultsThe plasma levels of Glc, TG, and MDA, the number of macrophages, and the percentage of fibrotic area in the kidneys of the LP/Cur/Fr group were significantly lower than those of the LP/LP/Fr group. The expression of Nrf2 and its downstream molecules HO‐1 and SOD1, GSH levels, and GPx activity in the kidneys of the LP/Cur/Fr group were significantly higher than those of the LP/LP/Fr group.ConclusionsMaternal curcumin intake during lactation may suppress oxidative stress by upregulating Nrf2 expression in the kidneys of fructose‐loaded female offspring exposed to maternal protein restriction.
Clinical efficacy analysis of butylphthalide combined with urinary kallidinogenase in treating chronic cerebral circulatory insufficiency
Authors: Hu Bo , Hu Feifan , Qiao Yulin 
Published: February 2023

AbstractObjectiveTo investigate the clinical effect of butylphthalide combined with urinary kallidinogenase in the treatment of chronic cerebral circulatory insufficiency (CCCI).MethodsA total of 102 CCCI patients admitted to our hospital from October 2020 to December 2021 were retrospectively enrolled in this study. According to the different therapeutic strategy, the patients were divided into combined group (treated with butylphthalide combined with urinary kallidinogenase, n = 51) and butylphthalide group (treated with butylphthalide, n = 51). Blood flow velocity and cerebral blood flow perfusion before and after treatment between the two groups were compared. The clinical efficacy and adverse events of the two groups were analyzed.ResultsAfter treatment, the effective rate of the combined group was significantly higher than the butylphthalide group (p = .015). Before treatment, the blood flow velocity of middle cerebral artery (MCA), vertebral artery (VA), basilar artery (BA) were comparable (p > .05, respectively), while after treatment, the blood flow velocity of MCA, VA, and BA in combined group were faster than those in butylphthalide group (p < .001, respectively). Before treatment, the relative cerebral blood flow (rCBF), relative cerebral blood volume (rCBV), relative mean transmit time (rMTT) of the two groups were comparable (p > .05, respectively). After treatment, rCBF and rCBV in combined group were higher than those in butylphthalide group (p < .001, respectively), and rMTT in combined group was lower than that in butylphthalide group (p = .001). The rate of adverse events in the two groups were comparable (p = .558).ConclusionButylphthalide combined with urinary kallidinogenase can improve the clinical symptoms of CCCI patients, and the effect is promising, which is worthy of clinical application.
Novel treatment approach of localized juvenile spongiotic gingival hyperplasia with use of Nd:YAG laser
Authors: Goldberg Adam S. , Fatahzadeh Mahnaz , Bortnik Volha , Drew Howard 
Published: February 2023

AbstractIntroductionThis article describes the management of two patients who presented with localized juvenile spongiotic gingival hyperplasia. This disease entity is poorly understood, and literature regarding successful treatment is scant. However, there are common themes to management including proper diagnosis and treatment via removal of the affected tissue. The biopsy demonstrates intercellular edema and neutrophil infiltrate with epithelial and connective tissue disease components, so surgical deepithelialization may not be adequate to definitively treat the disease.Methods and ResultsThis article presents two cases of the disease and suggests the use of the Nd:YAG laser as an alternative management modality.ConclusionTo our knowledge, we present the first cases of localized juvenile spongiotic gingival hyperplasia treated with the Nd:YAG laser.Key pointsWhy are these cases new information?

To our knowledge, this case series illustrates the first use of an Nd:YAG laser to treat the rare pathology localized juvenile spongiotic gingival hyperplasia.
What are the keys to successful management of these cases?

To properly manage this rare presentation, proper diagnosis is paramount. Following diagnosis with microscopic evaluation, deepithelialization and treatment of underlying connective tissue infiltrate via the Nd:YAG laser offers an elegant option to treat the pathology and maintain esthetic outcomes.
What are the primary limitations to success in these cases?

The primary limitations of these cases include the small sample size, which is attributable to the rarity of the disease.
Cell Wall‐Denaturing Molecules for Plant Gene Modification
Authors: Naka Risa , Tsuchiya Kousuke , Numata Keiji 
Published: February 2023

AbstractZwitterionic molecules, such as zwitterionic liquids (ZILs) and polypeptides (ZIPs), are attracting attention for application in new methods that can be used to loosen tight cell wall networks in a biocompatible manner. These novel methods can enhance the cell wall permeability of nanocarriers and increase their transfection efficiency into targeted subcellular organelles in plants. Herein, we provide an overview of the recent progress and future perspectives of such molecules that function as boosters for cell wall‐penetrating nanocarriers.
Revealing the Impact of Different Iron‐Based Precursors on the ‘Catalytic’ Graphitization for Synthesis of Anode Materials for Lithium Ion Batteries
Authors: Frankenstein Lars , Glomb Pascal , Ramirez‐Rico Joaquin , Winter Martin , Placke Tobias , Gomez‐Martin Aurora 
Published: February 2023

AbstractInvited for this issue's Front Cover is the group of Prof. Dr. Martin Winter from the MEET Battery Research Center (University of Münster, Germany). The cover picture shows two battery‐shaped coffee cups filled with coffee. In addition, the scientists (represented by gloved hands) have added both pure iron and an iron solution to the cups. The left cup filled with iron is highly energetic and strikes sparks, indicating better electrochemical performance and crystallinity than the right cup filled with the iron solution. The impact of activator addition prior to ‘catalytic’ graphitization and its effect on material and electrochemical properties is systematically investigated. Read the full text of the Research Article at 10.1002/celc.202201073.
Patient‐centered outcomes in the POLO study of active maintenance olaparib for germline BRCA‐mutated metastatic pancreatic cancer
Authors: Kindler Hedy L. , Yoo Hyun Kyoo , Hettle Robert , Cui Karen Y. , Joo Seongjung , Locker Gershon Y. , Golan Talia 
Published: February 2023

AbstractBackgroundThe phase 3 POLO study demonstrated a significant progression‐free survival (PFS) benefit and preserved health‐related quality of life (HRQOL) for active maintenance treatment with olaparib vs placebo in patients with metastatic pancreatic cancer and a germline BRCA mutation. Here, we present a post hoc analysis of the patient‐centered outcomes: time without significant symptoms of disease progression or toxicity (TWiST) and quality‐adjusted TWiST (Q‐TWiST).MethodsPatients were randomized 3:2 to maintenance olaparib (300 mg tablets twice daily) or placebo. Overall survival time was divided into TWiST, toxicity (TOX; time before disease progression with significant symptoms of toxicity), and relapse (REL; time after disease progression until death or censoring). Q‐TWiST was the sum of TWiST, TOX, and REL, each weighted by HRQOL utility scores during the relevant health‐state period. A base‐case and three sensitivity analyses were performed using differing definitions of TOX.ResultsIn total, 154 patients were randomized (olaparib, n = 92; placebo, n = 62). TWiST was significantly longer for olaparib than placebo in the base‐case analysis (14.6 vs 7.1 months; 95% CI, 2.9–12.0; p = .001) and all sensitivity analyses. No statistically significant benefit for Q‐TWiST was observed in the base‐case analysis (18.4 vs 15.9 months; 95% CI, −1.1 to 6.1; p = .171) or the sensitivity analyses.ConclusionThese results support the previous findings that maintenance olaparib significantly improves PFS relative to placebo without compromising HRQOL and demonstrate that the clinically meaningful benefits of olaparib persist even when symptoms of toxicity are considered.
Genetic diversity of colonial bentgrass Agrostis capillaris based on simple sequence repeat markers and high‐resolution melt analysis with haplotype scoring
Authors: Warnke Scott E. , Barnaby Jinyoung Y. 
Published: February 2023

AbstractColonial bentgrass (Agrostis capillaris) is a tetraploid rhizomatous bentgrass that generally exhibits improved resistance to the important fungal disease dollar spot and has more tolerance to water‐deficit stress than the more widely grown creeping bentgrass (Agrostis stolonifera). Interspecific hybridization between these two species is possible and efforts to understand the genetic mechanisms of enhanced biotic and abiotic stress resistance in colonial bentgrass are proceeding. To gain a better understanding of the level of genetic diversity in cultivated colonial bentgrasses and to determine if genotypes with improved interspecific hybridization potential can be identified, nine colonial bentgrass cultivars and one creeping bentgrass cultivar were screened with 48 colonial bentgrass‐derived simple sequence repeat markers. Twenty‐one primer pairs were found to produce reliable amplification, and these markers were scored using high‐resolution melt analysis to generate haplotypes that were used to evaluate cultivar relationships. The nine colonial bentgrass cultivars could be placed into two groups and overall showed limited levels of diversity. The creeping bentgrass cultivar was distinct and individual genotypes within each species exhibited haplotypes more common in the alternate species, suggesting that these markers may be useful for selecting genotypes with enhanced interspecific hybridization potential.
Genomic insights into the NPGS intermediate wheatgrass germplasm collection
Authors: Crain Jared , Larson Steve , Sthapit Sajal , Jensen Kevin , Poland Jesse , Dorn Kevin , Thomas Aaron , DeHaan Lee 
Published: February 2023

AbstractThe National Plant Germplasm System (NPGS) is a vital resource for genetic diversity, yet utilization of this resource requires a thorough understanding of the germplasm and genetic diversity. Intermediate wheatgrass (IWG, Thinopyrum intermedium) is a perennial grass species that has been improved for forage production through breeding utilizing the NPGS collection and has also been targeted for domestication as a perennial grain crop. To better characterize the IWG collection, we combined previously published forage data with new agronomic and genomic data. A total of 331 NPGS accessions were genomically profiled with genotyping‐by‐sequencing (GBS) and a genome‐wide association study (GWAS) was used to evaluate trait architecture. Along with the GWAS, in silico bulk samples were profiled by recoding GBS data to conduct association mapping through allele counting with extreme‐phenotype (XP)‐GWAS. Genomic analysis revealed two subpopulations, which were defined as European and Asian groups, and are differentiated around the Black Sea region. Phenotypic observations for forage and agronomic traits differed between the two groups (p < 0.05), even though greater than 70% of the genetic variance was partitioned within individual accessions. Finally, XP‐GWAS revealed 303 marker–trait associations for five agronomic and four forage traits. These results suggest that genetic diversity within the NPGS collection should lead to genetic gains for both forage and grain breeding as well as opportunities for breeding programs to enhance genetic diversity. More broadly, the methods we applied could be applicable to low‐resourced species, leveraging existing, and new data, to strengthen genetic characterization and breeding efficiency.
Seasonal plasticity in morphology and metabolism differs between migratory North American and resident Costa Rican monarch butterflies
Authors: Tenger‐Trolander Ayşe , Julick Cole R. , Lu Wei , Green Delbert André , Montooth Kristi L. , Kronforst Marcus R. 
Published: February 2023

AbstractEnvironmental heterogeneity in temperate latitudes is expected to maintain seasonally plastic life‐history strategies that include the tuning of morphologies and metabolism that support overwintering. For species that have expanded their ranges into tropical latitudes, it is unclear the extent to which the capacity for plasticity will be maintained or will erode with disuse. The migratory generations of the North American (NA) monarch butterfly Danaus plexippus lead distinctly different lives from their summer generation NA parents and their tropical descendants living in Costa Rica (CR). NA migratory monarchs postpone reproduction, travel thousands of kilometers south to overwinter in Mexico, and subsist on little food for months. Whether recently dispersed populations of monarchs such as those in Costa Rica, which are no longer subject to selection imposed by migration, retain ancestral seasonal plasticity is unclear. To investigate the differences in seasonal plasticity, we reared the NA and CR monarchs in summer and autumn in Illinois, USA, and measured the seasonal reaction norms for aspects of morphology and metabolism related to flight. NA monarchs were seasonally plastic in forewing and thorax size, increasing wing area and thorax to body mass ratio in autumn. While CR monarchs increased thorax mass in autumn, they did not increase the area of the forewing. NA monarchs maintained similar resting and maximal flight metabolic rates across seasons. However, CR monarchs had elevated metabolic rates in autumn. Our findings suggest that the recent expansion of monarchs into habitats that support year‐round breeding may be accompanied by (1) the loss of some aspects of morphological plasticity as well as (2) the underlying physiological mechanisms that maintain metabolic homeostasis in the face of temperature heterogeneity.
Comparison of mosquito and fly derived DNA as a tool for sampling vertebrate biodiversity in suburban forests in Berlin, Germany
Authors: Danabalan Renita , Planillo Aimara , Butschkau Susanne , Deeg Sita , Pierre Gras , Thion Cincia , Calvignac‐Spencer Sébastien , Kramer‐Schadt Stephanie , Mazzoni Camila 
Published: February 2023

AbstractThe use of invertebrate‐derived DNA (iDNA) is a promising non‐invasive tool to monitor wildlife. While most studies have been carried out in dense tropical and sub‐tropical forests and have focused on the use of a single category of invertebrates, this study compares the use of flies and mosquitoes‐derived DNA to assess vertebrate diversity in semi‐urban environments. We conducted our sampling in four different forest plots in Berlin, Germany. Pools of flies and non‐bloodfed mosquitoes were metabarcoded using 108‐bp vertebrate‐specific 12 S rRNA (12 S‐V5) and 94‐bp mammal‐specific 16 S rRNA (16Smam) mitochondrial markers, and individual bloodfed mosquitoes were sequenced using the 340‐bp vertebrate‐specific 12 S rRNA fragment (Mam‐12 S‐340). Most sequencing was only successful for mammal species. From the fly pools, we detected 10 mammal species using 16Smam, and six species using 12 S‐V5. From the non‐bloodfed mosquito pools, we only amplified putative contaminant DNA, indicating that mosquito females without visual signs of a blood meal carry no traces of vertebrate DNA. Finally, in the bloodfed mosquitoes, we identified four mammal species. We did not find significant differences in the proportion of mammal species detected regarding the total available number of species between sampling localities. Fly samples were easier to obtain and more abundant over the sampled localities compared to mosquito samples. We conclude that, while there are a few advantages in using mosquito blood meals, the use of flies in the detection of wildlife in a suburban environment is more effective in terms of collection of samples and detection of vertebrates, although this technique is limited to few mammal species in the urban environment.
Study on the impact of carbon emission pollution on urban tourism environment
Authors: Tian Ying 
Published: February 2023

AbstractThe tourism industry will continue to produce carbon emissions in the process of development, which will ultimately have an impact on the tourism environment. This article aims to study the effect of carbon emission pollution on the urban tourism environment. Taking Hebei Province from 2016 to 2020 as an example, the effect of carbon emission pollution on the local tourism environment was analyzed using the carbon emission model and tourism eco‐efficiency model. The local carbon emission increased in the process of developing the tourism industry; however, carbon emissions per unit in the accommodation, catering and shopping related to the tourism industry generated more tourism economic benefits. The authors suggest to strengthen the development of tourism transportation, increase local tourism promotion, enhance the diversity of tourism specialties, and improve the level of local catering and hotel services.
Metal impregnation on steel converter slag as an oxygen carrier
Authors: Hildor Fredrik , Mattisson Tobias , Linderholm Carl , Leion Henrik 
Published: February 2023

AbstractOxygen carriers used in chemical looping processes operated with biofuel are affected by the inorganic matter of the fuel. It is therefore expected that the lifetime of the oxygen carrier is limited, and preferably low‐cost oxygen carriers should be used. Oxygen carriers based on iron ore or steel manufacturing waste products are available in significant quantities at low cost. However, it is common for these types of materials that their reactivity is low. This study investigates the effect of adding small amounts of more reactive elements into steel converter slag, also called LD slag.Slag particles were wet impregnated with 2 or 5 wt.% of Ni, Cu, Mn, or Ce. The new material's morphology was evaluated using X‐Ray Diffraction and SEM‐EDS. Changes in reactivity towards CO, CH4 and the model tar molecule benzene were evaluated using a bench‐scale laboratory fluidized bed reactor.It was observed that even small amounts of either Ni, Cu, or Mn could increase reactivity toward CO. Both Cu and Mn formed phases with LD slag that released oxygen via CLOU (chemical looping with oxygen uncoupling) and increased the conversion of methane and benzene. Ni and Ce doping also increased methane conversion but had only a minor effect on the benzene conversion. © 2023 The Authors. Greenhouse Gases: Science and Technology published by Society of Chemical Industry and John Wiley & Sons Ltd.
The impact of cold waves and heat waves on mortality: Evidence from a lower middle‐income country
Authors: Nguyen Cuong Viet , Nguyen Manh‐Hung , Nguyen Toan Truong 
Published: February 2023

AbstractWe estimate the impact of temperature extremes on mortality in Vietnam, using daily data on temperatures and monthly data on mortality during the 2000–2018 period. We find that both cold and heat waves cause higher mortality, particularly among older people and those living in the hot regions in Southern Vietnam. This effect on mortality tends to be smaller in provinces with higher rates of air‐conditioning and emigration, and provinces with higher public spending on health. Finally, we estimate economic cost of cold and heat waves using a framework of willingness to pay to avoid deaths, then project the cost to the year 2100 under different Representative Concentration Pathway scenarios.
Prognostic value and clinicopathological status of PD‐L1 expression and CD8+ TILs in oral squamous cell cancer patients with or without traditional risk factors
Authors: Soopanit Thanion , Laokulrath Natthawadee , Chayopasakul Veeruth , Pongsapich Warut 
Published: February 2023

AbstractBackgroundNon‐smokers, non‐drinkers, and non‐betel quid chewers (NSNDNBs) with oral squamous cell carcinoma (OSCC) have poor survival outcomes. Tumor microenvironment based on PD‐L1/CD8+ T cell infiltrated lymphocyte (TIL) proportion is proposed as a prognostic indicator.MethodsImmunohistochemistry staining was performed on OSCC samples from 64 patients. The PD‐L1/CD8+ TILs were scored and stratified into four groups. Disease‐free survival (DFS) was analyzed using a Cox regression model.ResultsOSCC in NSNDNB patients was associated with female sex, T1‐2 classification, and PD‐L1 positivity. Low CD8+ TILs correlated with perineural invasion. High CD8+ TILs correlated with improved DFS. PD‐L1 positivity was not correlated with DFS. Type IV tumor microenvironment yielded the highest DFS (85%).ConclusionNSNDNB status relates to PD‐L1 expression regardless of CD8+ TILs infiltration. Type IV tumor microenvironment was associated with the best DFS. High CD8+ TILs resulted in better survival, while PD‐L1 positivity alone was not correlated to DFS.