bluuebunny/crossref_metadata_2025_split huggingface dataset:
Late embryo mortality in Holstein cows inseminated with Holstein or Limousine frozen semen
Authors: Melendez P. , Pinedo P. , Gibbons J. , Bartolome J. , Naikare H. 
Published: March 2023

AbstractThe objective of this study was to compare the late embryo mortality (LEM) rate (losses approximately between 32 and 53 days of gestation) and Pregnancy Specific Protein B (PSPB) and progesterone (P4) concentrations on day 32 post AI in Holstein cows bred with either Holstein or Limousine semen. A sample size of 1082 cows per group diagnosed pregnant between 28‐ and 35‐days post breeding was calculated. The study consisted of evaluating LEM (%) in a cohort of Holstein cows bred with Holstein semen (HO × HO) or Limousine semen (HO × LM), to compare pregnancy loss from 28 to 35 days post breeding to 50–57 days post breeding. A logistic regression model to compare embryo losses was developed considering as main explanatory variable the cohort (HO × HO embryo vs. HO × LM embryo), correcting by lactation number, breeding season, days to breeding and AI technician. HO × HO embryos had greater LEM (15.16%) than HO × LM embryos (9.79%). Cows bred in summertime had higher LEM (15.23%) than cows bred in no‐summertime (9.88%). There were no differences among AI technicians. Within summertime there was no difference in LEM (%) between groups within each lactation number; yet, within no‐summertime, LEM (%) was higher in HO × HO than HO × LM within each lactation number. Pregnancy SPB optical densities were significantly greater in the HO × HO than in the HO × LM (p = .023) group; yet, the concentration of P4 was not different between groups (p > .05).
Identifying the asthma research priorities of people with asthma, their carers and other stakeholders
Authors: Majellano Eleanor C. , Bell Rose L. , Flynn Anthony W. , Mckenzie Anne , Sivamalai Sundram , Goldman Michele , Vaughan Lauren , Gibson Peter G. 
Published: March 2023

AbstractBackground and ObjectivePeople living with asthma, their carers, clinicians and policymakers are the end‐users of research and need research that address their individual healthcare needs. We aimed to understand the research priorities of end‐users of asthma research.MethodsA national cross‐sectional mixed‐methods study was conducted. The study included an online survey that engaged patients, carers, healthcare professionals and policymakers to provide statements to free‐text questions about what they would like to see answered by research to improve living with asthma on a day‐to‐day basis. Responses where thematically analysed followed by three online priority setting consensus workshops.ResultsThere were 593 respondents who provided 1446 text comments. Participants prioritized 10 asthma research themes which were: (1) asthma in children, (2) COVID 19 and asthma, (3) asthma care and self‐management, (4) diagnosis and medication, (5) managing asthma attacks, (6) causes, prevention and features of asthma, (7) mental health, (8) asthma and ageing, (9) severe asthma, (10) asthma and other health conditions. Each theme comprises specific research questions.ConclusionThis project successfully established 10 priority research themes for asthma, reflecting the collective voice of the end‐users of this research. These novel data can be used to address the documented mismatch in research prioritization between the research community and the end‐users of research.
The VISA-A (sedentary) should be used for sedentary patients with Achilles tendinopathy: a modified version of the VISA-A developed and evaluated in accordance with the COSMIN checklist
Authors: Norris Richard , Cook Jill L , Gaida Jamie E , Maddox Thomas , Raju Jaya , O'Neill Seth 
Published: March 2023

ObjectiveTo develop and evaluate a modified version of the Victorian Institute of Sport Assessment-Achilles (VISA-A) questionnaire, for use in sedentary patients with Achilles tendinopathy, using the Consensus-based Standards for the selection of health Measurement Instruments recommendations.MethodsTwenty-two sedentary patients with Achilles tendinopathy completed the VISA-A and provided feedback regarding the relevance, comprehensiveness and comprehensibility of each item, response options and instructions. Patient and professional feedback was used to develop the VISA-A (sedentary) questionnaire. Reliability, validity and responsiveness of the VISA-A (sedentary) was evaluated in 51 sedentary patients with Achilles tendinopathy: 47.1% women, mean age 64.8 (SD 11.24).ResultsFactor analysis identified two dimensions (symptoms and activity) for the VISA-A (sedentary). Test–retest reliability was excellent for symptoms (intraclass correlation coefficient, ICC=0.991) and activity (ICC=0.999). Repeatability was 1.647 for symptoms and 0.549 for activity. There was a significant difference between the VISA-A and VISA-A (sedentary) scores both pretreatment and post-treatment. There was stronger correlation between the pretreatment to post-treatment change in the VISA-A (sedentary) scores (r=0.420 for symptoms, r=0.407 for activity) and the global rating of change than the VISA-A scores (r=0.253 for symptoms, r=0.186 for activity).ConclusionThe VISA-A (sedentary) demonstrates adequate reliability, validity and responsiveness in sedentary patients with Achilles tendinopathy. The VISA-A (sedentary) is a more appropriate measure than the VISA-A for this cohort and is recommended for clinical and research purposes.
Computerised clinical decision support system for the diagnosis of pulmonary thromboembolism: a preclinical pilot study
Authors: Altabbaa Ghazwan , Carpendale Sheelagh , Flemons Ward , Hemmelgarn Brenda , McLaughlin Kevin , Zuk Torre , Ghali William A 
Published: March 2023

BackgroundRecommendations for the diagnosis of pulmonary embolism are available for healthcare providers. Yet, real practice data show existing gaps in the translation of evidence-based recommendations. This is a study to assess the effect of a computerised decision support system (CDSS) with an enhanced design based on best practices in content and reasoning representation for the diagnosis of pulmonary embolism.DesignRandomised preclinical pilot study of paper-based clinical scenarios in the diagnosis of pulmonary embolism. Participants were clinicians (n=30) from three levels of experience: medical students, residents and physicians. Participants were randomised to two interventions for the diagnosis of pulmonary embolism: a didactic lecture versus a decision tree via a CDSS. The primary outcome of diagnostic pathway concordance (derived as a ratio of the number of correct diagnostic decision steps divided by the ideal number of diagnostic decision steps in diagnostic algorithms) was measured at baseline (five clinical scenarios) and after either intervention for a total of 10 clinical scenarios.ResultsThe mean of diagnostic pathway concordance improved in both study groups: baseline mean=0.73, post mean for the CDSS group=0.90 (p<0.001, 95% CI 0.10–0.24); baseline mean=0.71, post mean for didactic lecture group=0.85 (p<0.001, 95% CI 0.07–0.2). There was no statistically significant difference between the two study groups or between the three levels of participants.InterpretationA computerised decision support system designed for both content and reasoning visualisation can improve clinicians’ diagnostic decision-making.
Heterogeneity of tumor immune microenvironment of EGFR/ALK-positive tumors versus EGFR/ALK-negative tumors in resected brain metastases from lung adenocarcinoma
Authors: Xiao Gang , Li Lifeng , Tanzhu Guilong , Liu Zhiyuan , Gao Xuan , Wan Xin , Xiao Desheng , Chen Liu , Xia Xuefeng , Zhou Rongrong 
Published: March 2023

BackgroundPrevious studies found that lung adenocarcinomas (LUAD) with EGFR-positive and ALK-positive were less responsive to immunotherapy, which may be associated with a suppressive tumor immune microenvironment (TIME). Given the discordance in the TIME between primary lung cancer and brain metastasis, it is urgent to explore the TIME in patients with EGFR/ALK-positive LUAD with brain metastases (BMs).MethodsThe transcriptome feature of formalin-fixed and paraffin-embedded samples of BMs and paired primary LUAD from 70 patients with LUAD BMs was illustrated by RNA-sequencing. Six of them were available for paired sample analysis. Then, after excluding 3 co-occurring patients, we divided 67 BMs patients into 41 EGFR/ALK-positive and 26 EGFR/ALK-negative patients. The differences in immune profiling between the two groups were analyzed from three dimensions: TIME, T-cell receptor repertoire, and immunohistochemistry. Finally, the survival data of 55 patients were collected.ResultsCompared with primary LUAD, BMs present an immunosuppressed TIME, manifested as: inhibition of immune-related pathways; low expression of immune checkpoint; decreased infiltration of CD8+T cells and cytotoxic lymphocyte; increased proportion of suppressive M2 macrophages. In different subgroups based on EGFR/ALK gene variation status, both EGFR-positive and ALK-positive tumors present a relatively immunosuppressive microenvironment, but the heterogeneity of tumor microenvironment may undergo different mechanisms. EGFR-positive BMs showed decreased CD8+T cells and increased regulatory T cells (Treg) cells, while ALK-positive BMs showed decreased CD8+T cells and increased M2 macrophages. Moreover, in the TCGA-LUAD cohort, EGFR-positive tumors showed reduced CD8+T cell infiltrations (p<0.001) and borderline significantly higher Tregs than EGFR/ALK-negative (p=0.072). In parallel, ALK-positive tumors had higher median M2 macrophages infiltrations than EGFR/ALK-negative (p=0.175), although there was no statistical significance. Collectively, there was a similar immunosuppressive milieu between EGFR/ALK-positive primary LUAD and BMs. Moreover, survival analysis uncovered higher CD8A expression, cytotoxic lymphocyte infiltration, and immune scores were significantly associated with better prognosis in both EGFR/ALK-positive and EGFR/ALK-negative groups.ConclusionThis study found that LUAD-derived BMs exhibited an immunosuppressive TIME and revealed that EGFR-positive and ALK-positive BMs exhibited different immunosuppressive characteristics. Meanwhile, EGFR-negative BMs showed a potential benefit to immunotherapy. These findings boost molecular and clinical understanding of LUAD BMs.
Role of N-terminal pro-B type natriuretic peptide as a predictor of poor outcomes in patients with HFrEF receiving primary prevention implantable cardioverter-defibrillator therapy: a systematic review and dose–response meta-analysis
Authors: Prameswari Hawani Sasmaya , Putra Iwan Cahyo Santosa , Kamarullah William , Pranata Raymond , Iqbal Mohammad , Karwiky Giky , Pramudyo Miftah , Kusumawardhani Nuraini Yasmin , Achmad Chaerul , Martha Januar Wibawa , Akbar Mohammad Rizki 
Published: March 2023

IntroductionSeveral studies have demonstrated that combining left ventricular ejection fraction and New York Heart Association functional class is insufficient for predicting risk of appropriate implantable cardioverter-defibrillator (ICD) shock in primary prevention candidates. Hence, our aim was to assess the relationship between N-terminal pro-B type natriuretic peptide (NT-pro BNP) along with appropriate ICD shock and all-cause mortality in order to improve the stratification process of patients with heart failure with reduced ejection fraction (HFrEF) being considered for primary preventive ICD therapy.MethodsA systematic literature search from several databases was conducted up until 9 June 2022. Studies were eligible if they investigated the relationship of NT-pro BNP with all-cause mortality and appropriate ICD shock.ResultsThis meta-analysis comprised nine studies with a total of 5117 participants. Our study revealed that high levels of NT-pro BNP were associated with all-cause mortality (HR=2.12 (95% CI=1.53 to 2.93); p<0.001, I2=78.1%, p<0.001 for heterogeneity) and appropriate ICD shock (HR=1.71 (95% CI=1.18 to 2.49); p<0.001, I2=43.4%, p=0.102 for heterogeneity). The adjusted HR for all-cause mortality and appropriate ICD shock increased by approximately 3% and 5%, respectively per 100 pg/mL increment pursuant to concentration–response model (Pnon-linearity<0.001). The curves became steeper after NT-pro BNP reached its inflection point (3000 pg/mL).ConclusionA positive concentration-dependent association between elevated NT-pro BNP levels along with the risk of all-cause mortality and appropriate ICD shock was found in patients with HFrEF with ICD.PROSPERO registration numberCRD42022339285.
Type I interferon pathway assays in studies of rheumatic and musculoskeletal diseases: a systematic literature review informing EULAR points to consider
Authors: Burska Agata , Rodríguez-Carrio Javier , Biesen Robert , Dik Willem A , Eloranta Maija-Leena , Cavalli Giulio , Visser Marianne , Boumpas Dimitrios T , Bertsias George , Wahren-Herlenius Marie , Rehwinkel Jan , Frémond Marie-Louise , Crow Mary K , Ronnblom Lars , Conaghan PG , Versnel Marjan , Vital Ed 
Published: March 2023

ObjectivesTo systematically review the literature for assay methods that aim to evaluate type I interferon (IFN-I) pathway activation and to harmonise-related terminology.MethodsThree databases were searched for reports of IFN-I and rheumatic musculoskeletal diseases. Information about the performance metrics of assays measuring IFN-I and measures of truth were extracted and summarised. A EULAR task force panel assessed feasibility and developed consensus terminology.ResultsOf 10 037 abstracts, 276 fulfilled eligibility criteria for data extraction. Some reported more than one technique to measure IFN-I pathway activation. Hence, 276 papers generated data on 412 methods. IFN-I pathway activation was measured using: qPCR (n=121), immunoassays (n=101), microarray (n=69), reporter cell assay (n=38), DNA methylation (n=14), flow cytometry (n=14), cytopathic effect assay (n=11), RNA sequencing (n=9), plaque reduction assay (n=8), Nanostring (n=5), bisulphite sequencing (n=3). Principles of each assay are summarised for content validity. Concurrent validity (correlation with other IFN assays) was presented for n=150/412 assays. Reliability data were variable and provided for 13 assays. Gene expression and immunoassays were considered most feasible. Consensus terminology to define different aspects of IFN-I research and practice was produced.ConclusionsDiverse methods have been reported as IFN-I assays and these differ in what elements or aspects of IFN-I pathway activation they measure and how. No ‘gold standard’ represents the entirety of the IFN pathway, some may not be specific for IFN-I. Data on reliability or comparing assays were limited, and feasibility is a challenge for many assays. Consensus terminology should improve consistency of reporting.
Patterns of clinical joint inflammation in juvenile idiopathic arthritis
Authors: Heckert Sascha L , Hissink-Muller Petra C E , van den Berg J Merlijn , Schonenberg-Meinema Dieneke , van Suijlekom-Smit Lisette W A , van Rossum Marion A J , Koopman Yvonne , ten Cate Rebecca , Brinkman Danielle M C , Huizinga Tom W J , Allaart Cornelia F , Bergstra Sytske Anne 
Published: March 2023

ObjectivesWe studied patterns of joint inflammation in juvenile idiopathic arthritis (JIA) to assess whether joint activity recurs locally in the same joints.MethodsJoints of 91 patients of the BeSt for Kids study, a treat-to-target trial for children with recent-onset oligoarticular, rheumatoid factor-negative polyarticular and psoriatic JIA, were clinically assessed during 2 years (10 study visits). The association between joint inflammation at baseline and later inflammation in the same joint was assessed using a multilevel mixed-effects logistic regression model at joint level. With a Poisson model, the association between baseline joint inflammation and the number of study visits at which the same joint was recurrently inflamed was tested.ResultsOf the 6097 joints studied, 15% (897) was clinically inflamed at baseline. In 42% (377/897) of those joints, inflammation recurred during follow-up. Joint inflammation at baseline was statistically significantly associated with joint inflammation during follow-up in the same joint (OR 3.9, 95% CI 3.5 to 4.4) and specifically with the number of episodes of recurrent joint inflammation (IRR 1.6, 95% CI 1.2 to 2.1).ConclusionIn JIA, joint inflammation has the tendency to recur multiple times in joints that are clinically inflamed at disease onset. This indicates that local factors might play a role in the processes contributing to the occurrence of JIA flares.
Probing the non-standard neutrino interactions using quantum statistics
Authors: Kim C. S. , Rosiek Janusz , Sahoo Dibyakrupa 
Published: March 2023

AbstractUsing the well established principles of Lorentz invariance, CP and CPT symmetry, and quantum statistics we do a model-independent study of effects of possible non-standard couplings of (Dirac and Majorana) neutrinos. The study is sensitive to the different quantum statistical properties of the Dirac and Majorana neutrinos which, contrary to neutrino-mediated processes of lepton number violation, could lead to observable effects not suppressed by the small ratios of neutrino and heavier particle masses. For processes with a neutrino-antineutrino pair of the same flavor in the final state, we formulate the “Dirac Majorana confusion theorem (DMCT)” showing why it is normally very difficult to observe the different behaviour of both kinds of neutrinos in experiments if they have only the standard model (SM)-like left-handed vector couplings to gauge bosons. We discuss deviations from the confusion theorem in the presence of non-standard neutrino interactions, allowing to discover or constrain such novel couplings. We illustrate the general results with two chosen examples of neutral current processes, $$Z \rightarrow \nu \, {\overline{\nu }}$$

Z
→
ν

ν
¯

and $${\mathcal {P}}_i \rightarrow {\mathcal {P}}_f \, \nu \, {\overline{\nu }}$$

P
i

→

P
f

ν

ν
¯

(with $${\mathcal {P}}_{i,f}$$

P

i
,
f

denoting pseudoscalar mesons, such as $$B,K,\pi $$

B
,
K
,
π

). Our analysis shows that using 3-body decays the presence of non-standard interactions can not only be constrained but one can also distinguish between Dirac and Majorana neutrino possibilities.
Endothelial DLL4 Is an Adipose Depot–Specific Fasting Sensor Regulating Fatty Acid Fluxes
Authors: Aupetit Alex , Decaunes Pauline , Belles Chloé , Riant Elodie , Galitzky Jean , Chapouly Candice , Laisné Margaux , Flores-Flores Rémy , Chaput Benoit , Vié Katell , Arnal Jean-François , Bouloumié Anne , Briot Anaïs 
Published: March 2023

Background:
Adaptation of fat depots to change in fuel availability is critical for metabolic flexibility and cardiometabolic health. The mechanisms responsible for fat depot-specific lipid sensing and shuttling remain elusive. Adipose tissue microvascular endothelial cells (AT-EC) regulates bidirectional fatty acid fluxes depending on fed or fasted state. How AT-EC sense and adapt to metabolic changes according to AT location remains to be established.

Methods:
We combined transcriptional analysis of native human AT-EC together with in vitro approaches in primary human AT-EC and in vivo and ex vivo studies of mice under fed and fasted conditions.

Results:
Transcriptional large-scale analysis of human AT-EC isolated from gluteofemoral and abdominal subcutaneous AT revealed that the endothelium exhibits a fat depot–specific signature associated with lipid handling and Notch signaling enrichment. We uncovered a functional link between metabolic status and endothelial DLL4 (delta-like canonical notch ligand 4), which decreases with fasting. DLL4 regulates fatty acid uptake through nontranscriptional modulation of macropinocytosis-dependent long chain fatty acid uptake. Importantly, the changes in DLL4 expression, in response to energy transition state, is impaired under obesogenic conditions, an early alteration coinciding with a defect in systemic fatty acid fluxes adaptation and a resistance to weight loss.

Conclusions:
DLL4 is a major actor in the adaptive mechanisms of AT-EC to regulate lipid fluxes. It likely contributes to fat depot-dependent metabolism in response to energy transition states. AT-EC alteration with obesity may favor metabolic inflexibility and the development of cardiometabolic disorders.
FeelingBlue: A Corpus for Understanding the Emotional Connotation of Color in Context
Authors: Ananthram Amith , Winn Olivia , Muresan Smaranda 
Published: March 2023

Abstract
While the link between color and emotion has been widely studied, how context-based changes in color impact the intensity of perceived emotions is not well understood. In this work, we present a new multimodal dataset for exploring the emotional connotation of color as mediated by line, stroke, texture, shape, and language. Our dataset, FeelingBlue, is a collection of 19,788 4-tuples of abstract art ranked by annotators according to their evoked emotions and paired with rationales for those annotations. Using this corpus, we present a baseline for a new task: Justified Affect Transformation. Given an image I, the task is to 1) recolor I to enhance a specified emotion e and 2) provide a textual justification for the change in e. Our model is an ensemble of deep neural networks which takes I, generates an emotionally transformed color palette p conditioned on I, applies p to I, and then justifies the color transformation in text via a visual-linguistic model. Experimental results shed light on the emotional connotation of color in context, demonstrating both the promise of our approach on this challenging task and the considerable potential for future investigations enabled by our corpus.1
Hybrid Regionalism in Africa
Authors: Krösche Niklas 
Published: March 2023

Abstract
Since its establishment, the African Union (AU) takes on an active role in regional security matters through different types of interventions. These interventions, however, remain undertheorized. This paper argues that African hybrid regionalism, which combines problem-solving and regime-serving logics of cooperation, shapes the AU’s intervention practice in specific ways. To this end, I first theorize how the parallel presence of these logics shapes AU interventions before probing the empirical validity by studying coercive interventions undertaken by the AU Peace and Security Council (PSC) between 2005 and 2021. For this purpose, I employ methods of content analysis to systematically code all publicly available meeting documents issued by the PSC. The results demonstrate that the AU strives to prevent and manage crises through interventions but does so in ways that protect or promote incumbent regimes, either by producing direct benefits for them or, when their actions contribute to the crisis, by avoiding head-on confrontations. This suggests careful balancing of the two main impetuses in African security regionalism, namely solving transnational problems and serving the interests of incumbents.
Huns in the East: The Polychrome Style
Authors: Kazakov Aleksandr , Kazakova Olga 
Published: March 2023

Abstract
The Great Migration period is closely connected with the culture of ancient multi- ethnic society known as the Huns. Although the Hunnic archaeology is well developed, there are still many issues to be solved. One of them is where was the eastern border of the Hunnic culture marking the areas of their influence. The article tries to answer the question and presents materials, which at present are the most eastern finds of polychrome style objects that became markers for the Huns’ burials. They were found on the Eras’ka River burial in the Kalmanskii District of the Altai Territory, Russia. The authors consider technological methods used by craftsmen in the Great Migration period for making polychrome style jewelry.