bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of TAB182 promotes cancer stem-like cell properties and therapeutic resistance in triple-negative breast cancer cells
Authors: He Huan , Wang Shaozheng , Zhang Wen , Gao Shanshan , Guan Hua , Zhou Pingkun 
Published: April 2023

Abstract
Table 182 participates in DNA damage repair and radio-/chemo-sensitivity regulation in various tumors, but its role in tumorigenesis and therapeutic resistance of breast cancer remains unclear. In the current paper, we observed that Triple-Negative Breast Cancer (TNBC), a highly aggressive type of breast cancer, exhibits a lower expression of TAB182. TAB182 knockdown stimulates cell proliferation, migration, and invasion in TNBC cells. Our study first constructed the RNA-seq data to explore the cellular functions of TAB182 at the genome level in TNBC cells. Transcriptome analysis and in vitro experiments identify that TAB182 downregulation drives the enhanced properties of cancer stem-like cells (CSCs) in TNBC cells. Furthermore, TAB182 deletion contributes to the resistance of cells to Olaparib or Cisplatin, which could be rescued by silencing GLI2, a downstream gene of cancer stemness-related signaling pathways. Our results reveal a novel function of TAB182 as a significant negative regulator of cancer stem-like properties and drug sensitivity in TNBC cells, suggesting TAB182 may be a tumor suppressor gene and associated with more therapeutic benefits for TNBC.
Development of Automatic Orbital Pipe
MIG Welding System and Process
Parameters Optimization of AISI 1020 Mild
Steel Pipe Using Hybrid Artificial Neural
Network and Genetic Algorithm
Authors: Mengistie Amanuel Kassa , Bogale Teshome Mulatie 
Published: April 2023

Abstract
Currently, in pipe welding, it is nearly difficult for a human welder to weld
the whole circumference of a pipe in a single uninterrupted pass using
MIG welding causing inconsistencies in weld quality around the welded
pipe. The aim of this study was to develop an automated-orbital pipe
MIG welding system and to optimize welding parameters for enhancing
ultimate tensile strength and Rockwell hardness of mild steel 1020 grade
pipe. Three levels of variation were applied to the four input parameters
that were chosen. Nine experiments were carried out using orthogonal
array of L9. In this experimental investigation, the highest ultimate tensile
strength (UTS) of 411.2 MPa and Rockwell hardness (RH) of 95
HRB were achieved at 110 A of current and 24 V of voltage, welding
gun travel speed of 30 cm/min and 3 mm of arc length. For modeling
the orbital pipe MIG welding process experimental input parameters and
response results, a hybrid ANN-GA model was constructed. This model
was used to forecast and optimize ultimate tensile strength and Rockwell
hardness, as well as the process factors that go with it. The results
indicated that the ANN-GA model could predict the output responses
with a mean absolute error of 5.06e-05. During optimization, a 4-9-2
network trained with neural network of back propagation by Bayesian
regularization approach was determined to have the greatest prediction
capability, with maximum UTS and RH of 417.857 MPa and 96.5364
HRB, respectively. The predicted and confirmation test results were
both found within the acceptable errors, according to a confirmation
test conducted with the optimum parameters of the ANN-GA model.
Development of a spectrophotometric method for the quantification of c-phycocyanin in the cyanobacteria Aphanizomenon flos-aquae
Authors: Billy Julie , Pruvost Jeremy , Lépine Olivier , Drouin Delphine , Gonçalves Olivier 
Published: April 2023

Abstract
The current study presents the development of a reliable method for the quantification of c-phycocyanin. It was found that the spectrophotometric method commonly used for c-phycocyanin quantification tends to overestimate the actual amount of c-phycocyanin in AFA samples. Thus, the aim of this study was to account for c-phycocyanin variation between cyanobacteria species in order to reliably adapt the spectrophotometric quantification method of c-phycocyanin for Aphanizomenon flos-aquae (AFA). High performance liquid chromatography (HPLC) was used to avoid interference between molecules. The existing spectrophotometric equations for the quantification of AFA c-phycocyanin were adapted using a c-phycocyanin standard. The method was then used to obtain a new set of spectrophotometric quantification equations that were adapted to the strain of interest and ensured the accuracy of c-phycocyanin quantification while continuing to use a rapid, simple, and inexpensive method for pigment quantification. The method developed here could be adapted to improve the quantification methods for other types of phycocyanin, cyanobacteria, or even other compounds of interest that are currently quantified by spectrophotometry.
Spatial genetic structure of two conifers in a highly modified landscape of central Mexico
Authors: Cruz-Salazar Bárbara , Flores-Manzanero Alejandro 
Published: April 2023

Abstract
Forests have been globally reduced due to unprecedented rates of deforestation and habitat transformation, which in turn affects the species’ genetic structure. Despite harboring a high endemism, temperate forests are the second most degraded terrestrial ecosystem in Mexico. Therefore, understanding the spatial genetic patterns is fundamental to implement management and conservation efforts that contribute with their long-term persistence. By using Single Nucleotide Polymorphisms, this study evaluated the fine-scale spatial genetic structure and the effect of landscape features on gene flow of two conifers, Abies religiosa and Pinus montezumae, each with different successional affinity, in a temperate forest of central Mexico exhibiting high landscape heterogeneity due to topography and land-use changes. Based on successional affinity of species we expected isolation by resistance in Abies religiosa and a panmictic population in Pinus montezumae. We analyzed the genetic structure and the effects of altitude, Aspect and land-use changes on the genetic connectivity patterns. A weak isolation by distance pattern was detected in A. religiosa, while the spatial component explained only the 3% of its genetic variation. In P. montezumae, a very low structure signal was detected between slopes. Overall, the analyses revealed an absence of genetic structure in both species, i.e., no landscape predictor had an effect on gene flow between the populations. We recommend increasing the spatial extent and the assessment of more environmental predictors to further support the study of genetic structure patterns in the species studied. Our study contributes with information for the conservation of Mexican temperate forests.
Valorisation of buttermilk in mixotrophic and heterotrophic conditions by using the acidophilic strain Galdieria sulphuraria
Authors: Occhipinti Paride Salvatore , Signore Flavia Del , Canziani Stefano , Caggia Cinzia , Mezzanotte Valeria , Ferrer-Ledo Narcís 
Published: April 2023

Abstract
The growth of the polyextremophile red microalga Galdieria sulphuararia ACUF064 under mixotrophy and heterotrophy was evaluated in a lab-scale stirred photobioreactor, using buttermilk as carbon source. Beforehand, G. sulphuraria growth on glucose, galactose and lactose, was evaluated. The final biomass concentration of G. sulphuraria was about 2 and 1.5 times lower in mixotrophy and heterotrophy, respectively, when G. sulphuraria ACUF064 was grown on lactose rather than in glucose or galactose. No significant difference was observed in mixotrophy between growth in glucose and galactose. C-phycocyanin content was about 1.6 and 1.37 times higher in mixotrophy on lactose than on galactose and on glucose. In heterotrophy, C-phycocyanin content, on lactose was 1.57 and 1.56 times higher than values detected in glucose and galactose, respectively. The preliminary mixotrophic flask experiment with different buttermilk dilutions showed that the highest biomass production was obtained at 1:2.5 dilution rate, corresponding to 40% v/v of butter milk and to 2.5 gC L− 1. On buttermilk, the highest biomass production was obtained in mixotrophy, at 1:2.5 dilution rate, in a 13 L photobioreactor where the algal productivity was 0.55 g L− 1 d− 1, and no contamination occurred at pH 1.6.
Dynamic performance of newly developed environmentally friendly greases containing polysaccharide gums in rolling bearing
Authors: Saxena Ankit , Kumar Deepak , Tandon Naresh 
Published: April 2023

Abstract

The present study explores the performance of novel vegetable oil-based greases containing polysaccharide gums as additives. Two series of greases with varying concentrations of gum acacia (GA) and guar gum (GG) are evaluated in a rolling bearing at various speeds and loads. The vibrations and shock pulse measurements (SPM) quantified the tribo-dynamics of the developed greases against two commercial greases used as the benchmark. The vibration and SPM assessment suggest that the newly developed eco-friendly greases are at par with the commercial ones; the performance varies with the concentration and type of gum. Where a cumulative effect of rheology and gum size seems to influence the vibrations, the entrainment of gums in the contact zone governs the SPM carpet values. The developed greases show great prospects as a sustainable, potential, and commercial alternative to existing environmentally unfriendly greases.
Serum metabolic signatures for Alzheimer’s Disease reveal alterations in amino acid composition and energy metabolism – A validation study
Authors: Nielsen Jonas Ellegaard , Andreassen Trygve , Gotfredsen Charlotte Held , Olsen Dorte Aalund , Vestergaard Karsten , Madsen Jonna Skov , Kristensen Søren Risom , Pedersen Shona 
Published: April 2023

Abstract
Background: Alzheimer’s Disease (AD) is complex and novel approaches are urgently needed to characterise disease pathology and to aid in diagnosis. Metabolites are the end-products of upstream molecular alterations, whereby small changes at the genetic or protein level result in substantial changes at the metabolite level. Blood is frequently used as a source for biomarkers; however, its complexity prevents proper detection. The analytical power of metabolomics, coupled with statistical tools, can assist in reducing this complexity. Furthermore, the current bottleneck in biomarker research is reproducibility and appropriate validation. Thus, we sought to validate a previously proposed panel of metabolic blood-based biomarkers for AD and expand our understanding of the pathological mechanisms involved in AD that are reflected in the blood.
Methods: In the validation cohort serum and plasma were collected from 25 AD patients and 25 healthy controls. Serum was analysed for metabolites using nuclear magnetic resonance (NMR) spectroscopy, while plasma was tested for markers of neuronal damage and AD hallmark proteins using single molecule array (SIMOA). A combination of multivariate and univariate statistics were utilized to validate established biomarkers and uncover new disease-related evidence.
Results: The diagnostic performance of the proposed metabolite biomarker panel was confirmed using sparse-partial least squares discriminant analysis (sPLS-DA) with an area under the curve (AUC) of 0.89 (95 % confidence interval: 0.79 – 0.98). Five metabolites (pyruvic acid, valine, leucine, histidine, and isoleucine) were consistently reduced in both the discovery and validation cohorts. Pathway analysis of significantly altered metabolites in the validation set revealed that they are involved in branched-chain amino acids (BCAAs) and energy metabolism (glycolysis and gluconeogenesis). Additionally, a moderate correlation was observed between valine and the proteins neurofilament light and glialfibrillary acidic protein. By combining the significant protein expression levels measured by SIMOA with the sPLS-DA model, the AUC increased to 0.97 (95 % CI: 0.93 – 1.00).
Conclusions: Our proposed panel of metabolites was successfully validated using a combined approach of NMR and sPLS-DA. It was discovered that cognitive-impairment-related metabolites belong to BCAAs and are involved in energy metabolism.
Lautropia mirabilis-induced peritoneal dialysis-associated peritonitis: a case report
Authors: Liu Shuhui , Yan Yufen , Hu Changcan , Huang Xiuling , Liu Qing , Liu Yanfei , Jia Nan , Han Chunhua 
Published: April 2023

Abstract
Background
Lautropia mirabilis is a Gram-negative bacterium mainly isolated from the mouth and upper respiratory tract. To date, only two cases of peritoneal dialysis-associated peritonitis induced by Lautropia mirabilis have been globally reported: one in Australia and one in Portugal, but Lautropia mirabilis has not been cultured. Here, we report the third case of peritoneal dialysis-associated peritonitis caused by Lautropia mirabilis in the world. Furthermore, we cultivated this bacterium to improve the existing knowledge on the etiology of this condition and provide clinical guidance for its treatment.
Case presentation:
A 49-year-old female with renal insufficiency for more than five years was admitted to our hospital. She had been on peritoneal dialysis for six months. The patient had diarrhea and turbid peritoneal dialysis fluid for three days. With indication of infection by routine results of peritoneal dialysis fluid, mass spectrometer identification and gene sequencing of peritoneal dialysis fluid culture were subjected and revealed infection with Lautropia mirabilis. After anti-infection treatment with gentamicin and ceftazidime, the patient recovered.
Conclusions
Our findings suggest that Lautropia mirabilis plays an important role in the pathogenesis of peritoneal dialysis-associated peritonitis. Early diagnosis and timely antibiotic treatment may be essential for the treatment of this infection.
Vacuum Assisted Closure Therapy in Children with Complicated Abdominal Wounds, with or without Viscero-Cutaneous Fistulae
Authors: ghandour mostafa el , naggar osama el , radwan ahmed , abdel-latif mohammed 
Published: April 2023

Abstract
Background:Management of complicated abdominal wounds is challenging in children. Vacuum-assisted closure proved to be beneficial. We aimed to report the outcome of VAC over 5 years’ practice in complicated abdominal wounds with or without viscero-cutaneous fistulae.
Methods:Retrospective review of children managed by VAC over 5 years, from January 2017 to December 2022. Included patients had complicated abdominal wounds. Customized VAC devices with continuous negative pressure were used for all patients. The applied pressure was tailored according to the patient’s age and the wound condition.
Results:Nineteen patients were included, their ages ranged from 3 months to 15 years. Peritonitis was the main pathology in 12 patients, abdominal wall infection in 4, and a combination of both in 3. VAC was applied over closed wounds in 10 patients and over open wounds in 9; 6 of them had cutaneous fistulae. The median duration of VAC application over closed wounds was 7.5 days ,while it was 10 days in open wounds. VAC promoted healing in closed and narrow-gapped wounds, while it was successfully used as a bridging therapy in 4 patients with wide-gapped wounds. Patients with viscero-cutaneous fistulae were successfully managed without further surgical intervention. VAC was complicated by fascial dehiscence with evisceration and incisional hernia in two patients.
Conclusion:VAC could be beneficial to complicated abdominal wounds in pediatric age. It was helpful in managing small viscero-cutaneous fistulae.
Interleukin-17 regulates keratinocyte proliferation in psoriasis through the MALAT1/miR-125b/ BRD4 axis
Authors: Pan Min , Zhu Xiao-fang , Chen Yi-he , Lin Xiang-fei , Wang Xiaokang 
Published: April 2023

Abstract
Background: Psoriasis is a common chronic inflammatory dermatosis characterized by scaly erythema and plaques. This study explored the specific mechanism of LncRNA MALAT1 in the occurrence and development of psoriasis.
Methods: We collected serum from psoriasis patients and healthy subjects and measured the expression of IL-17 in serum by ELISA. HaCaT cells were stimulated with 80 ng/mL IL-17 and simultaneously transfected with shMALAT1 or were treated with Secukinumab (anti-IL-17A). The CCK-8 and EdU experiments were used to detect cell viability and proliferation, flow cytometry showed the level of cell cyle, and corresponding cyclins were measured by WB and RT-QPCR, and changes in a series of LncRNAs were detected. A luciferase activity analysis and ChIP assay verified the targeting relationship between MALAT1 and p65.
Results: IL-17 treatment of HaCaT cells promoted cell proliferation and increased MALAT1 expression. The binding of p65 to the promoter of MALAT1 promoted keratinocyte proliferation. The proliferation of HaCaT cells induced by IL-17 was repressed by Secukinumab. The expression of miR-125b was notably reduced in cells treated with IL-17, whereas Secukinumab rescued the expression of miR-125b. IL-17 treatment resulted in elevated BRD4 expression, which was attenuated by Secukinumab.
Conclusions: IL-17 stimulates HaCaT cell proliferation by inducing high expression of MALAT1, and p65 binds to the promoter of MALAT1 to promote its expression, which may provide a novel direction for the treatment of psoriasis.
Research on the relationship between asset structure and enterprise growth ability of listed automobile companies: Evidence from China
Authors: Jiang Allen 
Published: April 2023

Abstract
In China's national economy, the auto industry has played a crucial role in stimulating the economy and promoting employment. Assets are the foundation of the production and operation activities of automobile manufacturing enterprises. Different asset structures (AS) will bring different levels of income and risk, and there will be differences in the impact on the growth of enterprises. This paper constructs a comprehensive index evaluation system of enterprise growth ability (GA) with 17 evaluation indicators in six dimensions: profitability, operating ability, risk response-ability, market growth ability, innovation ability and human capital. The variables of AS are determined from the two dimensions of total AS and current AS, and the relationship between AS and enterprise GA is tested by multiple regression analysis. This study found that the proportion of current assets is significantly positively correlated with the enterprise GA in listed companies in China's auto industry in the total AS. The proportion of fixed assets is negatively correlated with the enterprises GA. The proportion of intangible assets is slightly negatively correlated with the GA of current enterprises, and significantly positively correlated with the future GA. In the current AS, the proportion of monetary capital is significantly positively correlated with the GA of enterprises, while the proportion of inventory is significantly negatively correlated with the GA of enterprises.
PointCMC: Cross-Modal Multi-Scale Correspondences Learning for Point Cloud Understanding
Authors: Zhou Honggu , Peng Xiaogang , Luo Yikai , Wu Zizhao 
Published: April 2023

Abstract

Existing cross-modal frameworks have achieved impressive performance in point cloud representation learning, where a 2D image encoder is employed to transfer knowledge to a 3D point cloud encoder. However, the local structures between point clouds and corresponding images are unaligned, which results in a challenge for the 3D point cloud encoder to learn fine-grained image-point cloud interactions. In this paper, we introduce a novel multi-scale training strategy (PointCMC) to enhance fine-grained cross-modal knowledge transfer in the cross-modal framework. Specifically, we design a Local-to-Local (L2L) module that implicitly learns the correspondence of local features by aligning and fusing extracted local feature sets. Moreover, we introduce the Cross-Modal Local-Global Contrastive (CLGC) loss, which enables the encoder to capture discriminative features by reasoning local structures to their corresponding cross-modal global shape. The extensive experimental results demonstrate that our approach outperforms the previous unsupervised learning methods in various downstream tasks such as 3D object classification and semantic segmentation.
Brain metastases in adult patients with melanoma of unknown primary in the Netherlands (2011–2020)
Authors: Padilla C. S. , Ho V. K.Y. , Mooijenkind T. W.A.N. , Louwman M. W.J. , Vos F. Y. F. L , Bekkenk M. W. , Minnaard W. F . , Loef C. , Zanten S.E.M Veldhuijzen van 
Published: April 2023

Abstract
Background
Although patients with melanoma of unknown primary (MUP) have a better prognosis than similar-staged melanoma patients with known primary, the occurrence of brain metastases (BM) entails a serious complication. This study provides an overview of the incidence, treatment patterns, and overall survival (OS) of adult patients with BM-MUP in the Netherlands.
Methods
BM-MUP cases were retrieved from the Netherlands Cancer Registry. Patient, disease and treatment-related characteristics were summarised using descriptive statistics. Overall survival (OS) was calculated by the Kaplan-Meier method, and the impact of prognostic factors on OS was assessed using Cox proportional hazard regression analyses.
Results
Among 1,779 MUP patients, 450 were identified as BM-MUP (25.3%). Of these patients, 381 (84.7%) presented with BM along with other metastases, while 69 (15.3%) had BM only. BM-MUP patients were predominantly male (68.2%), and had a median age of 64 years at diagnosis (interquartile range 54–71 years). Over time, the proportion of BM along other metastatic sites increased, and the occurrence of BM decreased (p = 0.01). 1-Year OS improved for the total population, from 30.0% (95% confidence interval (CI): 19.8–40.9%) in 2011–2012 to 43.6% (95%CI: 34.5–52.3%) in 2019–2020, and median OS more than doubled from 4.2 months (95%CI: 3.3–6.2 months) to 9.8 months (95%CI: 7.0–13.2 months). Patient’s age, localisation of BM, presence of synchronous liver metastasis and treatment were identified as independent predictors of OS.
Conclusion
Notwithstanding the progress made in OS for patients with BM-MUP in the past decade, their overall prognosis remains poor, and further efforts are needed to improve outcomes.
Leukemia relapse via genetic immune escape after allogeneic hematopoietic cell transplantation
Authors: Pagliuca Simona , Gurnari Carmelo , Hercus Colin , Hergalant Sébastien , Hong Sanghee , Dhuyser Adele , D’Aveni Maud , Aarnink Alice , Rubio Marie Thérèse , Feugier Pierre , Ferraro Francesca , Carraway Hetty E. , Sobecks Ronald , Hamilton Betty K. , Majhail Navneet S. , Visconte Valeria , Maciejewski Jaroslaw P. 
Published: April 2023

Abstract
Graft-versus-leukemia (GvL) reactions are responsible for the effectiveness of allogeneic hematopoietic cell transplantation as a treatment modality for myeloid neoplasia, whereby donor T- effector cells recognize leukemia neoantigens. However, a substantial fraction of patients experience relapses because of the failure of the immunological responses to control leukemic outgrowth. Here, through a broad immunogenetic study, we demonstrate that germline and somatic reduction of human leucocyte antigen (HLA) heterogeneity enhances the risk of leukemic recurrence. We show that preexistent germline-encoded low evolutionary divergence of class II HLA genotypes constitutes an independent factor associated with disease relapse and that acquisition of clonal somatic defects in HLA alleles may lead to escape from GvL control. Both class I and II HLA genes are targeted by somatic mutations as clonal selection factors potentially impairing cellular immune reactions and response to immunomodulatory strategies. These findings define key molecular modes of post-transplant leukemia escape contributing to relapse.