bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set
Authors: Fujino Tomoshige , Sonoda Ryogo , Murakami Hiroshi 
Published: September 2023

Abstract
The Ser/Leu-swapped genetic code can act as a genetic firewall, mitigating the biohazard risks arising from horizontal gene transfer in genetically modified organisms. Our prior work demonstrated the orthogonality of this swapped code to the standard genetic code using a cell-free translation system comprised of 21 in vitro transcribed tRNAs. To advance this system for protein engineering, we introduced a natural/in vitro transcribed hybrid tRNA set. This set combined natural tRNAs from Escherichia coli (excluding Ser, Leu, and Tyr) and in vitro transcribed tRNAs, encompassing anticodon-swapped tRNASerGAG and tRNALeuGGA. This approach reduced the requisite in vitro transcribed tRNAs from 21 to a mere 4. In this optimized system, the production of a model protein, super-folder green fluorescent protein, increased to 3.5-fold. With this hybrid tRNA set, the Ser/Leu-swapped cell-free translation system will stand as a potent tool for protein production for reduced biohazard concerns in future biological endeavors.
Identity development, attraction, and behaviour of heterosexually-identified men who have sex with men: A scoping review
Authors: Eaton Andrew D. , Scheadler Travis R. , Kon Taylor , Pang Nelson , Kwan Sandra , McDonald Mel , Dillon Frank R. , McInroy Lauren B. , Beer Oliver W. J. , Beckwell Erin , Busch Adam , Vandervoort Daniel , Bradley Cara , Shuper Paul A. 
Published: September 2023

Abstract

Heterosexually identified men who have sex with men (H-MSM) experience discordance between their sexual identity and behaviours. Studies with large, mixed samples suggest that H-MSM may comprise 4–7% of the sexually active adult male population. Understanding and accepting H-MSM as they self-identify may be necessary to implement effective public health and psychosocial interventions. There is no known knowledge synthesis of H-MSM. This scoping review synthesizes primary studies about H-MSM identity development, attraction, and behaviour. Thirteen databases were searched, and two independent reviewers screened 3,617 titles and abstracts and 269 full-texts to arrive at 120 articles meeting inclusion criteria. Ten independent reviewers then conducted thematic content analysis. H-MSM either expressed sexual identity uncertainty, or justified maintaining heterosexual identity, due to fear of discrimination and little or no social support. H-MSM compartmentalized same-sex sexual behaviours as isolated events unrepresentative of their sexual identity. H-MSM further minimized these behaviours to infrequent, recreational/sport, or economic coincidences with little partner communication regarding HIV and sexual health. Many H-MSM also depersonalized male sex partners, denied same-sex attraction, and avoided gay-identified venues. Reviewed articles further reported H-MSM had negative emotional responses to sex with men (e.g., guilt, shame, disgust). Findings also suggest H-MSM are mislabeled in sexual health screening. H-MSM are unlike other heterosexual men and other MSM, and require unique considerations and approaches to sexual and mental health care.
ReportNeedles.ca: A real-time needle collection tool to foster community health partnerships
Authors: Eaton Andrew D. , Pang Nelson , Varghese Shiny Mary , Reddy Vidya Dhar , Ross Sarah , Novotna Gabriela , Beckwell Erin , Medeiros Priscilla , Shuper Paul A. , Ibàñez-Carrasco Francisco 
Published: September 2023

Abstract

Technology-mediated interactions between the public, healthcare agencies, and researchers can be a facilitator of community health partnerships. The use of such novel technologies can lead to innovations in public health research to address disparities and access issues. This article presents a web-based, real-time needle collection tool ReportNeedles.ca and describes its use in an ongoing community health partnership to deploy and evaluate pop-up interventions for blood-borne infection prevention and substance use harm reduction. Since April 2021, 34,350 needles have been collected from 466 public reports on the ReportNeedles.ca app in the city of Regina, Saskatchewan (population: ~215,000). This non-walkable city with pronounced needle prevalence may be representative of medium-sized cities in Canada, the United States, and elsewhere, where brick-and-mortar healthcare is predominantly accessible only to people of relative stability. This article discusses the tool’s development, implementation, and evaluation plan alongside its potential for blood-borne infection, substance use, and community-based participatory research.
The budgeted maximin share allocation problem
Authors: Li Weidong , Deng Bin 
Published: September 2023

Abstract
We are given a set of indivisible goods and a set of n agents where each good has a size and each agent has an additive valuation function and a budget. The budgeted maximin share allocation problem is to find a feasible allocation such that the size of the bundle allocated to each agent does not exceed its budget, and the minimum ratio of the valuation and the maximin share (MMS) value of any agent is as large as possible, where the MMS value of each agent is that he can achieve by dividing the goods into n bundles, and receiving his least desirable bundle.
In this paper, we prove that the existence of 1/3 -approximate MMS allocation and give an instance which does have a (3 /4 + ε)-approximate MMS allocation, for any ε ∈ (0, 1). Moreover, we provide a polynomial time algorithm to find an ( 1/3 − ε)-approximate MMS allocation, and prove that there is no polynomial time algorithm which can find a ( 2/3 + ε)-approximate MMS allocation for any instance unless P = N P.
To adopt or adapt an existing neonatal core outcome set in Kenya: A study protocol
Authors: Karumbi Jamlick , Gathara David , Young Bridget , Williamson Paula 
Published: September 2023

Abstract
Background
Development and use of core outcome set(s) (COS) in research can reduce research wastage by ensuring that a minimum set of outcomes are always reported on. Neonatal morbidity and mortality are a big burden in low- and middle-income countries (LMICs). Research is continuously being undertaken to reduce this burden. Currently there is no COS for neonatal research in LMICs but there exists one for neonatal research in high income countries (HICs).
Objectives
To determine outcomes that are useful for neonatal care in Kenya to inform whether an existing COS should be adopted or adapted. To assess feasibility of a routine data collection system to collect data of the agreed-on COS.
Methods
A review of existing literature on neonatal research in LMICs followed by qualitative study of key stakeholders in neonatal care. To explore whether to adapt or adopt, in two hospitals, two focus group discussions with 6–8 parents/caregivers will be undertaken (one each in two hospitals). Key informant interviews will also be conducted with 6 health care providers in each of the hospitals. At the policy making level, we will conduct 10 key informant interviews. Qualitative data will be analyzed thematically. A consensus meeting will be undertaken with key stakeholders, who will be presented with an overview of the COS developed for HICs, key findings from the literature and the qualitative study to determine context appropriate COS. The agreed-on outcomes will be counterchecked against the case records in the two hospitals. Feasibility of collecting the outcomes on a routine electronic research database, the Clinical Information Network that collects standardized data at admission and discharge will be explored. The congruence (or not) of the outcomes will be documented and be used to enrich the discussion and provide a snapshot of the feasibility of the health information system to collect routine data on the COS.
Conclusions
A COS for use in neonatal care in Kenya will help enhance outcome measurements and reporting not just in research but also in routine practice. This will enhance comparability of interventions in trials and routine setting leading to reduced research wastage and likely improved quality of care. Additionally, the methodology used for this work can be adopted in other settings as a means of adopting or adapting an existing COS.
О СУДЬБЕ ЗАУДАРНЫХ ДОЛГОТ В РЕЧИ БАСТАСА
Authors: Козомара Драгомир В. 
Published: September 2023

На основании собранного диалектного материала о речи Бастаса, деревни на юго-западе Боснии, автор статьи отмечает, что по распределению заударных долгот эта речь очень похожа на стандартный сербский язык и другие речи восточно-герцеговинского (герцеговинско-краинского) диалекта, к группе которого она принадлежит по своей структуре. Основные отклонения от просодической нормы сербского языка в речи Бастаса сводятся на пример к следующему:
1) Заударные долготы утрачиваются на гласной е в основе настоящего времени у многочисленных глаголов первой группы Стевановича, если глагольные формы, построенные из такой основы, имеют долгое восходящее ударение: извéземо гоблéнā, сȁмо ỳ кāртама крάде.
2) Они также утрачиваются в форме настоящего времени у глаголов с основой на -je: нѐ вјерујем ником, засије свѐгā пὸмало.
3) Вторичные долготы регулярно встречаются в притяжательных формах, оканчивающихся на на -ов и -ин: на Бȍгдāнов пóзив, Нȅнāд Бȍшков; Нéђо Ȁнūн, Àњђēлкūн ỳнук.
4) Кроме того, вторичные долготы регулярно встречаются на последнем слоге слова в третьем лице множественного числа аориста: зὰвадͧшē се, јутрōс закὰснͧшē...
В работе также описывается отношение речи Бастаса к соседним икавским говорам ливанско-дуванского типа.
ENGLISH INDEFINITE PRONOUN ANY AND ITS SERBIAN TRANSLATION EQUIVALENTS
Authors: Pantić Irena M. 
Published: September 2023

This study aims to analyse a contrastive relation between the English indefinite pronoun any and its Serbian equivalents. Beside the simple pronoun any, the study also focuses on how its compounds with other words – nouns (-body, -thing), numerals (-one) and interrogatives (-where) are performed and translated into Serbian. In this sense, the study is result-oriented. In order to reveal the equivalence relevance and equivalence distribution in practice, the analysis was based on a parallel corpus. The source text ʻThe Sense of an Endingʼ by J. Barnes was confronted with the corresponding target text in Serbian ʻOvo liči na krajʼ, to investigate the appropriate forms.
Although belonging to different language families, English and Serbian have shown a significant degree of similarities and differences among the elements in scope. Indefinite pronouns are a linguistic universal. Thus they occur systematically in both English and Serbian. Nevertheless, the Serbian pronominal equivalents are not categorised only as indefinites, but they also have a negative (niko, ništa, nikakav), free-choice (neko, nešto, neki) and universal functions26 (svako, bilo koji, bilo kakav). There is rarely any reciprocity in a direct Serbian implication for each English pronoun – an English indefinite pronoun usually has numerous Serbian equivalents. The proportion of the pronominal and non-pronominal (adverbs, particles, prepositional phrases, etc) Serbian equivalents is 2:1. Zero equivalence has appeared to be unexpectedly frequent – predominantly in negative and interrogative contexts. But even when the indefinites are not explicitly translated, due to stylistic and pragmatic differences between the two languages, there is equivalence at a syntactic level. The analysis revealed various equivalents, but they can be roughly brought down to the following: any is mostly omitted in Serbian negatives, and interrogatives, and translated as svaki in affirmatives; anything is translated as ništa in negatives, išta in interroatives and nešto in affirmatives; anyone is translated as niko in negatives, and iko in affirmatives; anybody is translated as bilo ko in affirmatives; anyway is translated as bilo kako bilo in affirmatives; anywhere is translated as ikud in negatives. Such an asymmetry of equivalence shows that there is not obvious and apparent compatibility between English and Serbian indefinite pronouns.
The aim of this paper has therefore been to offer a better understanding of the various forms among the corresponding English and Serbian indefinite pronominals, in terms of their practical relevance.
REVIEW OF (CRITICAL) LITERATURE ABOUT BILINGUALISM
Authors: Dragaš Mina Z. 
Published: September 2023

The topic of bilingualism has always been an object of interest in linguistic circles, especially today in the time of globalisation or the “third epoch”, when borders of states haven’t always implied the boundary of the use of a particular language. In addition to the fact that research on this phenomenon covers a wide range of hypotheses and perspectives from which bilingualism is analysed, it increasingly involves developing countries, multicultural and multilingual environments. Numerous scientists have investigated the process of first and second language acquisition, some have observed in which environments and under what conditions bilingualism occurs, and this group includes scientists who have done research on bilingual children in the school system whose attitudes will be stated in this paper.
This work provides an overview of the basic conclusions from theoretical and empirical studies on the topic of bilingualism, through a review of research in which attention is drawn to the traditionally neglected or insufficiently explored extralinguistic factors and their influence on the development or obstruction of bilingualism. The aim is to come to a conclusion about the different competences of bilingual children and the conditions that affect the development of bilingualism in early childhood and in children of school age. Some of the definitions of bilingualism are primarily listed, then the functions of language as well as the relationship between the first and second languages, the influence of parents and culture on the early development of bilingualism are explained. In the final part of the paper, practical advice for helping bilingual children is given, based on a literature review on this topic. Therefore, we conclude that the article discusses current and scientifically relevant issues: types of bilingualism, as well as its socio-pedagogical implications.
Incorporating economy and water demand rate uncertainty into decision-making for agricultural water allocation during droughts
Authors: Liu Hu , Liu Ning , Zhang Danli , Zhu Jianzi , Zhu Yonghua 
Published: September 2023

Abstract
Determining sources of uncertainty in the agricultural water supply helps to increase irrigation scheduling accuracy. Evaporation and transpiration, sensitivity coefficient of water tension, cultivation costs, product price and water allocated to plants are among the main sources of uncertainty in the simulation process. This study was conducted to estimate the fluctuations of the response due to the uncertainty of four parameters of soil moisture, water provided for cultivation, cost of cultivation during the growing season, and coefficient of sensitivity to drought. In the concept of water and food nexus, two basic factors, including increasing economic productivity and achieving maximum product production, are known as decision criteria. Therefore, mathematical modeling of net income and the ratio of actual and potential yield production has been prepared according to the four uncertain input variables of the problem. The proposed model has used a multi-objective particle swarm optimization algorithm to estimate the best values of non-deterministic variables. The results of the uncertainty analysis showed that the range of changes in performance and net profit compared to the coefficient of sensitivity to water stress has different ranges and mechanisms and knowing the nature of each helps to improve the responses.
Pilot-scale study of UVC-based AOPs towards implementation at the outlet of domestic WWTPs
Authors: Gabet Anaëlle , Monot Thomas , Mailhot Gilles , Brigante Marcello , de Brauer Christine , Métivier Hélène 
Published: September 2023

Abstract

The degradation of a mixture of ibuprofen, naproxen, and diclofenac in various effluents by UVC/H2O2 or UVC/S2O82− was studied to assess the impact of the matrix composition and of the oxidant precursor on process efficiency. Experiments were carried out in a 20-L laboratory pilot (a scaled-down version of a full-scale pilot). In effluents collected during dry weather, the rural constructed wetland effluent allowed faster degradation than the urban conventional WWTP effluent, regardless of the nature of the targets or of the oxidant precursor. This was mainly attributed to a three-times higher chemical oxygen demand in the urban effluent, likely to quench the oxidative species. UV fluences to reach 90% degradation of the three compounds were 3,800 and 5,500 mJ cm−2 in the rural effluent, whereas they were 6,600 and 6,100 mJ cm−2 in the urban effluent with H2O2 and S2O82−, respectively. After a rainfall event, the rural effluent composition was not significantly affected compared to that of the urban effluent that underwent the dilution effect. Therefore, the stability of the rural effluent composition allowed comparable degradation efficiency, whereas the dilution effect led to a significant increase in the degradation rate constants in the urban effluent (up to four times higher).
The Application of MD Simulation to Lead Identification, Vaccine Design,
and Structural Studies in Combat against Leishmaniasis - A Review
Authors: Vijayakumar Saravanan , Kumar Lukkani Laxman , Borkotoky Subhomoi , Murali Ayaluru 
Published: September 2023

Abstract:
Drug discovery, vaccine design, and protein interaction studies are rapidly moving toward
the routine use of molecular dynamics simulations (MDS) and related methods. As a result of MDS, it
is possible to gain insights into the dynamics and function of identified drug targets, antibody-antigen
interactions, potential vaccine candidates, intrinsically disordered proteins, and essential proteins. The
MDS appears to be used in all possible ways in combating diseases such as cancer, however, it has not
been well documented as to how effectively it is applied to infectious diseases such as Leishmaniasis.
As a result, this review aims to survey the application of MDS in combating leishmaniasis. We have
systematically collected articles that illustrate the implementation of MDS in drug discovery, vaccine
development, and structural studies related to Leishmaniasis.

:
Of all the articles reviewed, we identified that only a limited number of studies focused on the development
of vaccines against Leishmaniasis through MDS. Also, the PCA and FEL studies were not
carried out in most of the studies. These two were globally accepted utilities to understand the conformational
changes and hence it is recommended that this analysis should be taken up in similar approaches
in the future.
QSAR Studies and Scaffold Optimization of Predicted Novel ACC 2
Inhibitors to Treat Metabolic Syndrome
Authors: Madan Kirtika , Paliwal Sarvesh , Sharma Swapnil , Kesar Seema , Chauhan Neha , Madan Mansi 
Published: September 2023

Background:
Metabolic syndrome is one of the major non-communicable global health
hazards of the modern world owing to its amplifying prevalence. Acetyl coenzyme-A carboxylase 2
(ACC 2) is one of the most crucial enzymes involved in the manifestation of this disease because of its
regulatory role in fatty acid metabolism.

Objective:
To find novel potent ACC 2 inhibitors as therapeutic potential leads for combating metabolic
syndrome.

Methods:
In the present study, a two-dimensional quantitative structure-activity relationship (2D
QSAR) approach was executed on biologically relevant thiazolyl phenyl ether derivatives as ACC 2
inhibitors for structural optimization. The physiochemical descriptors were calculated and thus a correlation
was derived between the observed and predicted activity by the regression equation. The significant
descriptors i.e. log P (Whole Molecule) and Number of H-bond Donors (Substituent 1) obtained
under study were considered for the design of new compounds and their predicted biological
activity was calculated from the regression equation of the developed model. The compounds were
further validated by docking studies with the prepared ACC 2 receptor.

Results:
The most promising predicted leads with the absence of an H-bond donor group at the substituted
phenyl ether moiety yet increased overall lipophilicity exhibited excellent amino acid binding
affinity with the receptor and showed predicted inhibitory activity of 0.0025 μM and 0.0027 μM. The
newly designed compounds were checked for their novelty. Lipinski's rule of five was applied to
check their druggability and no violation of this rule was observed.

Conclusion:
The compounds designed in the present study have tremendous potential to yield orally
active ACC 2 inhibitors to treat metabolic syndrome.
Comprehensive Review on Drug-target Interaction Prediction - Latest
Developments and Overview
Authors: Abdul Raheem Ali K. , Dhannoon Ban N. 
Published: September 2023

Abstract:
Drug-target interactions (DTIs) are an important part of the drug development process.
When the drug (a chemical molecule) binds to a target (proteins or nucleic acids), it modulates the biological
behavior/function of the target, returning it to its normal state. Predicting DTIs plays a vital
role in the drug discovery (DD) process as it has the potential to enhance efficiency and reduce costs.
However, DTI prediction poses significant challenges and expenses due to the time-consuming and
costly nature of experimental assays. As a result, researchers have increased their efforts to identify
the association between medications and targets in the hopes of speeding up drug development and
shortening the time to market. This paper provides a detailed discussion of the initial stage in drug discovery,
namely drug–target interactions. It focuses on exploring the application of machine learning
methods within this step. Additionally, we aim to conduct a comprehensive review of relevant papers
and databases utilized in this field. Drug target interaction prediction covers a wide range of applications:
drug discovery, prediction of adverse effects and drug repositioning. The prediction of drugtarget
interactions can be categorized into three main computational methods: docking simulation approaches,
ligand-based methods, and machine-learning techniques.