bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
Authors: Fernández-Veiga Leire , Fuertes Miguel , Geijo María V. , Pérez de Val Bernat , Vidal Enric , Michelet Lorraine , Boschiroli María Laura , Gómez-Buendía Alberto , Bezos Javier , Jones Gareth J. , Vordermeier Martin , Juste Ramón A. , Garrido Joseba M. , Sevilla Iker A. 
Published: February 2023

AbstractThe single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity.
Asymmetric response of South Asian summer monsoon rainfall in a carbon dioxide removal scenario
Authors: Zhang Suqin , Qu Xia , Huang Gang , Hu Peng 
Published: February 2023

AbstractThe reversibility of South Asian summer monsoon (SASM) precipitation under the CO2 removal scenario is critical for climate mitigation and adaptation. In the idealized CO2 ramp-up (from 284.7 to 1138.8 ppm) and symmetric ramp-down experiments, SASM precipitation is largely reversible while exhibiting strong asymmetry: it may overshoot the unperturbed level when CO2 recovers. Such asymmetric response is mainly due to the enhanced El Niño-like and Indian Ocean dipole-like warming during the ramp-down period. The uneven sea surface warming weakens Walker circulation, with anomalous sinking over the SASM region. Meanwhile, the warming also affects the rainfall over the Maritime Continent and tropical western Indian Ocean. The suppressed rainfall over the Maritime Continent triggers the equatorial Rossby wave, which weakens the ascent over the SASM region; the increased rainfall over the tropical western Indian Ocean excites the equatorial Kelvin wave, which reduces moisture transport. Additionally, tropic-wide warming reduces the land-sea thermal contrast and weakens monsoonal circulation. Consequently, the combined effects of the weakened ascent and moisture transport lead to the overshooting of SASM rainfall. Our results suggest that symmetric CO2 removal, although unlikely in the foreseeable future, may result in a risk of local drought over the SASM region.
Pan-cancer clinical impact of latent drivers from double mutations
Authors: Yavuz Bengi Ruken , Tsai Chung-Jung , Nussinov Ruth , Tuncbag Nurcan 
Published: February 2023

AbstractHere, we discover potential ‘latent driver’ mutations in cancer genomes. Latent drivers have low frequencies and minor observable translational potential. As such, to date they have escaped identification. Their discovery is important, since when paired in cis, latent driver mutations can drive cancer. Our comprehensive statistical analysis of the pan-cancer mutation profiles of ~60,000 tumor sequences from the TCGA and AACR-GENIE cohorts identifies significantly co-occurring potential latent drivers. We observe 155 same gene double mutations of which 140 individual components are cataloged as latent drivers. Evaluation of cell lines and patient-derived xenograft response data to drug treatment indicate that in certain genes double mutations may have a prominent role in increasing oncogenic activity, hence obtaining a better drug response, as in PIK3CA. Taken together, our comprehensive analyses indicate that same-gene double mutations are exceedingly rare phenomena but are a signature for some cancer types, e.g., breast, and lung cancers. The relative rarity of doublets can be explained by the likelihood of strong signals resulting in oncogene-induced senescence, and by doublets consisting of non-identical single residue components populating the background mutational load, thus not identified.
Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration
Authors: Yu Hongping , Wang Yanyi , Gao Junjie , Gao Youshui , Zhong Chao , Chen Yixuan 
Published: February 2023

AbstractThe brain-bone regulatory system regulates skeletal homeostasis via bioactive neuropeptides, yet the underlying mechanism remains elusive. Here, we report the role of the neuropeptide VF (NPVF, VPNLPQRF-NH2) in enhancing both angiogenesis and osteogenesis in a rat skeletal system and the potential pathways involved. An in vitro study revealed that NPVF not only promotes migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) by activating NPFFR1, which leads to upregulation of miR-181c-3p and downregulation of Argonaute1 (AGO1), but also mediates osteogenic differentiation of bone mesenchymal stem cells (BMSCs) via the Wnt/β-catenin signaling pathway. To improve the stability and bioavailability and thus efficacy of NPVF as a promoter of in vivo bone regeneration, we genetically engineered amyloid-NPVF-fusion proteins and utilized them as self-assembling nanofiber coatings to treat bone defects in a rat calvarial defect model. We found that a porous hydroxyapatite scaffold loaded with the NPVF peptide-fused amyloid coating substantially enhanced angiogenesis and site-specific fresh bone in-growth when implanted in calvarial defects. Taken together, our work uncovered a previously undefined crosstalk between the brain and bone by unveiling the role of NPVF in bone tissue and demonstrated a viable method for promoting bone tissue repairs based upon self-assembling NPVF-containing protein coatings.
Measuring health and human development in cities and neighborhoods in the United States
Authors: Sheth Suraj K. , Bettencourt Luís M. A. 
Published: February 2023

AbstractHuman development is a complex process involving interactions between individuals and their socioeconomic, biological, and physical environments. It has been studied using two frameworks: the “Capabilities Approach,” implemented at the national scale, and the “Neighborhood Effects Approach,” implemented at the community scale. However, no existing framework conceptualizes and measures human development across geographic scales. Here, we unite the two approaches by localizing the Human Development Index (HDI), and demonstrate a methodology for scalable implementation of this index for comparative analysis. We analyzed patterns of development in the United States, characterizing over 70,000 communities. We found that, on average, larger cities have higher HDI (higher standard of living) but exhibit greater disparities between communities, and that increases in community HDI are associated with the simultaneous reduction of a diverse set of negative neighborhood effects. Our framework produces an interdisciplinary synthesis of theory and practice for sustainable, equitable urban health and development.
Higher concentrations of microplastics in runoff from biosolid-amended croplands than manure-amended croplands
Authors: Naderi Beni Nasrin , Karimifard Shahab , Gilley John , Messer Tiffany , Schmidt Amy , Bartelt-Hunt Shannon 
Published: February 2023

AbstractLand-applied municipal biosolids, produced from municipal wastewater treatment sludge, contributes to microplastics contamination in agroecosystems. The impacts of biosolids on microplastic concentrations in agricultural soil have been previously investigated, however, the potential for microplastics transport from biosolid-amended croplands has not been previously quantified. In this study, manure and biosolids were applied to field plots, runoff was collected following natural precipitation events and the potential of bacterial biofilm to grow on different microplastic morphologies was investigated. Higher concentrations of microplastics were detected in runoff from plots with land-applied biosolid in comparison with manure-amended and control plots. Fibers and fragments were the most frequently detected plastic morphologies in runoff, correlated with their decreased surface roughness. The potential of biosolids to contribute to microplastic contamination to U.S. surface waters was quantified which is among the first to quantify the potential for nonpoint source microplastic contamination of surface waters adjacent to agricultural production areas.
Neuronal APOE4 removal protects against tau-mediated gliosis, neurodegeneration and myelin deficits
Authors: Koutsodendris Nicole , Blumenfeld Jessica , Agrawal Ayushi , Traglia Michela , Grone Brian , Zilberter Misha , Yip Oscar , Rao Antara , Nelson Maxine R. , Hao Yanxia , Thomas Reuben , Yoon Seo Yeon , Arriola Patrick , Huang Yadong 
Published: February 2023

AbstractApolipoprotein E4 (APOE4) is the strongest known genetic risk factor for late-onset Alzheimer’s disease (AD). Conditions of stress or injury induce APOE expression within neurons, but the role of neuronal APOE4 in AD pathogenesis is still unclear. Here we report the characterization of neuronal APOE4 effects on AD-related pathologies in an APOE4-expressing tauopathy mouse model. The selective genetic removal of APOE4 from neurons led to a significant reduction in tau pathology, gliosis, neurodegeneration, neuronal hyperexcitability and myelin deficits. Single-nucleus RNA-sequencing revealed that the removal of neuronal APOE4 greatly diminished neurodegenerative disease-associated subpopulations of neurons, oligodendrocytes, astrocytes and microglia whose accumulation correlated to the severity of tau pathology, neurodegeneration and myelin deficits. Thus, neuronal APOE4 plays a central role in promoting the development of major AD pathologies and its removal can mitigate the progressive cellular and tissue alterations occurring in this model of APOE4-driven tauopathy.
Chronic in vivo imaging defines age-dependent alterations of neurogenesis in the mouse hippocampus
Authors: Wu Yicheng , Bottes Sara , Fisch Roberto , Zehnder Cinzia , Cole John Darby , Pilz Gregor-Alexander , Helmchen Fritjof , Simons Benjamin D. , Jessberger Sebastian 
Published: February 2023

AbstractNeural stem cells (NSCs) generate new neurons throughout life in the mammalian hippocampus1. Advancing age leads to a decline in neurogenesis, which is associated with impaired cognition2,3. The cellular mechanisms causing reduced neurogenesis with advancing age remain largely unknown. We genetically labeled NSCs through conditional recombination driven by the regulatory elements of the stem-cell-expressed gene GLI family zinc finger 1 (Gli1) and used chronic intravital imaging to follow individual NSCs and their daughter cells over months within their hippocampal niche4,5. We show that aging affects multiple steps, from cell cycle entry of quiescent NSCs to determination of the number of surviving cells, ultimately causing reduced clonal output of individual NSCs. Thus, we here define the developmental stages that may be targeted to enhance neurogenesis with the aim of maintaining hippocampal plasticity with advancing age.
Structural elucidation and hypoglycemic effect of an inulin-type fructan extracted from Stevia rebaudiana roots
Authors: Tang Zhiyan , Shao Taili , Gao Lan , Yuan Pingchuan , Ren Zhengrui , Tian Lei , Liu Wei , Liu Chunyan , Xu Xiuxian , Zhou Xuan , Han Jun , Wang Guodong 
Published: February 2023

A novel fructan (SRRP) from S. rebaudiana roots ameliorates glucose and lipid metabolism disorders in type 2 diabetic mice via gut microbiota regulation.
Acoustic Measures of Dysphonia in Amyotrophic Lateral Sclerosis
Authors: Maffei Marc F. , Green Jordan R. , Murton Olivia , Yunusova Yana , Rowe Hannah P. , Wehbe Farah , Diana Kathleen , Nicholson Katharine , Berry James D. , Connaghan Kathryn P. 
Published: February 2023

Purpose:
Identifying efficacious measures to characterize dysphonia in complex neurodegenerative diseases is key to optimal assessment and intervention. This study evaluates the validity and sensitivity of acoustic features of phonatory disruption in amyotrophic lateral sclerosis (ALS).

Method:
Forty-nine individuals with ALS (40–79 years old) were audio-recorded while producing a sustained vowel and continuous speech. Perturbation/noise-based (jitter, shimmer, and harmonics-to-noise ratio) and cepstral/spectral (cepstral peak prominence, low–high spectral ratio, and related features) acoustic measures were extracted. The criterion validity of each measure was assessed using correlations with perceptual voice ratings provided by three speech-language pathologists. Diagnostic accuracy of the acoustic features was evaluated using area-under-the-curve analysis.

Results:
Perturbation/noise-based and cepstral/spectral features extracted from /a/ were significantly correlated with listener ratings of roughness, breathiness, strain, and overall dysphonia. Fewer and smaller correlations between cepstral/spectral measures and perceptual ratings were observed for the continuous speech task, although post hoc analyses revealed stronger correlations in speakers with less perceptually impaired speech. Area-under-the-curve analyses revealed that multiple acoustic features, particularly from the sustained vowel task, adequately differentiated between individuals with ALS with and without perceptually dysphonic voices.

Conclusions:
Our findings support using both perturbation/noise-based and cepstral/spectral measures of sustained /a/ to assess phonatory quality in ALS. Results from the continuous speech task suggest that multisubsystem involvement impacts cepstral/spectral analyses in complex motor speech disorders such as ALS. Further investigation of the validity and sensitivity of cepstral/spectral measures during continuous speech in ALS is warranted.
Reporting of Classroom-Based Morphological Awareness Instruction and Intervention for Kindergarten to Grade 3 Students in the Literature: A Scoping Review
Authors: Passaretti Basiliki , Turkstra Lyn S. , Gallagher Tiffany , Jiang Annie , Cahill Peter , Campbell Wenonah 
Published: February 2023

Purpose:
The purpose of this scoping review was to document how the literature reports morphological awareness instruction and interventions delivered by speech-language pathologists (SLPs) and/or educators in classroom settings for kindergarten to Grade 3 students.

Method:
We followed the Joanna Briggs Institute's methodology for scoping reviews and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews reporting guidelines. Six relevant databases were searched systematically with article screening and selection completed by two reviewers calibrated for reliability. For data charting, one reviewer extracted content and a second reviewer verified it was pertinent to the review question. Charting for the reported elements of morphological awareness instruction and interventions was guided by the Rehabilitation Treatment Specification System.

Results:

The database search yielded 4,492 records. After removal of duplicates and screening, 47 articles were selected for inclusion. Interrater reliability for source selection exceeded the pre-established criterion of
k
= .61. Our analysis generated a comprehensive description of the elements of morphological awareness instruction as reported in the included articles.

Conclusions:
Our findings provide school-based SLPs and educators a systematic means of reviewing the literature to identify key elements of morphological awareness instruction in published articles for application of evidence-based practices with fidelity, thus helping to close the research-to-practice gap. Our manifest content analysis revealed reporting of the elements for classroom-based morphological awareness instruction was varied, and in some cases, underspecified in the articles included in our study. Implications for clinical practice and future research to advance knowledge and promote implementation of evidence-based practices by SLPs and educators in today's classrooms are discussed.

Supplemental Material:

https://doi.org/10.23641/asha.22105142
Robust state estimation for uncertain linear discrete systems with d‐step measurement delay and deterministic input signals
Authors: Tian Yu , Meng Fanli , Mao Yao , Gao Junwei , Liu Huabo 
Published: February 2023

AbstractIn this study, the state estimation problems for linear discrete systems with uncertain parameters, deterministic input signals and d‐step measurement delay are investigated. A robust state estimator with a similar iterative form and comparable computational complexity to the Kalman filter is derived based on the state augmentation method and the sensitivity penalisation of the innovation process. It is discussed that the steady‐state properties such as boundedness and convergence of the robust state estimator under the assumptions that the system parameters are time invariant. Numerical simulation results show that compared with the Kalman filter, the obtained state estimator is more robust to modelling errors and has nice estimation accuracy.
A rapid prediction model of photovoltaic power generation for autonomous long‐duration aerostat
Authors: Liu Qianshi , Xu Guoning , Li Zhaojie , Gao Yang , Yang YanChu , Li Yongxiang , Du Hao 
Published: February 2023

AbstractAutonomous long‐duration aerostats (LDA) are one of the most popular research directions of high‐altitude platforms (HAPS) in recent years. Solar photovoltaic (PV) array is the energy source of autonomous long‐duration aerostat, whose power generation predicting accuracy and speed affect the subsequent flight control strategy. Limited by incompleteness cognition of near space, current predicting results cannot meet the requirements of autonomous LDA. In this paper, a novel rapid prediction model of the PV array is proposed. Based on spatial position relation of PV cells, this model can predict the power of single PV cell in any state. The four influence factors including time difference, angle, efficiency loss and temperature are analyzed and optimized comprehensively and innovatively. The new model can be applied in both static state aerostat and dynamic state aerostat. Compared with the traditional model, the efficiency can be improved by 50% and the prediction accuracy can be improved by 10%.
Day‐ahead continuous double auction‐based peer‐to‐peer energy trading platform incorporating trading losses and network utilisation fee
Authors: Liaquat Sheroze , Hussain Tanveer , Celik Berk , Fourney Robert , Hansen Timothy M. 
Published: February 2023

AbstractIntegration of distributed energy resources, such as photovoltaic solar (PV), introduces new opportunities to establish local energy market frameworks to improve renewable energy utilisation in residential sectors. Such peer‐to‐peer (P2P) energy trading refers to a local market structure where customers (and prosumers) interact to share excess PV generation to enhance the individual and community social welfare. In this work, a day‐ahead continuous double auction (CDA)‐based P2P market structure considering network losses and network utilisation fees was designed. Day‐ahead PV energy is modelled using fractional integral polynomials and the output is forecasted using an autoregressive integrated moving average model for each market interval. Based on the customer load and excess PV energy, the CDA market is cleared using a bid/ask matching mechanism. The performance of the P2P market was evaluated by computing different welfare metrics while analysing the effect of network constraints. The results show that the designed CDA‐based P2P market structure increases the social welfare of all participants by an average of 17.75% compared to the baseline for the presented cases. Moreover, the impact of the forecasting error between the day‐ahead and real‐time market was also quantified.
The IACOB project
Authors: Britavskiy N. , Simón-Díaz S. , Holgado G. , Burssens S. , Maíz Apellániz J. , Eldridge J. J. , Nazé Y. , Pantaleoni González M. , Herrero A. 
Published: February 2023

Context. The empirical distribution of projected rotational velocities (v sin i) in massive O-type stars is characterised by a dominant slow velocity component and a tail of fast rotators. It has been proposed that binary interaction plays a dominant role in the formation of this tail.
Aims. We perform a complete and homogeneous search for empirical signatures of binarity in a sample of 54 fast-rotating stars with the aim of evaluating this hypothesis. This working sample has been extracted from a larger sample of 415 Galactic O-type stars that covers the full range of v sin i values.
Methods. We used new and archival multi-epoch spectra in order to detect spectroscopic binary systems. We complemented this information with Gaia proper motions and TESS photometric data to aid in the identification of runaway stars and eclipsing binaries, respectively. We also benefitted from additional published information to provide a more complete overview of the empirical properties of our working sample of fast-rotating O-type stars.
Results. The identified fraction of single-lined spectroscopic binary (SB1) systems and apparently single stars among the fast-rotating sample is ∼18% and ∼70%, respectively. The remaining 12% correspond to four secure double-line spectroscopic binaries (SB2) with at least one of the components having a v sin i > 200 km s−1 (∼8%), along with a small sample of 2 stars (∼4%) for which the SB2 classification is doubtful: these could actually be single stars with a remarkable line-profile variability. When comparing these percentages with those corresponding to the slow-rotating sample, we find that our sample of fast rotators is characterised by a slightly larger percentage of SB1 systems (∼18% vs. ∼13%) and a considerably smaller fraction of clearly detected SB2 systems (8% vs. 33%). Overall, there seems to be a clear deficit of spectroscopic binaries (SB1+SB2) among fast-rotating O-type stars (∼26% vs. ∼46%). On the contrary, the fraction of runaway stars is significantly higher in the fast-rotating domain (∼33–50%) than among those stars with v sin i < 200 km s−1. Lastly, almost 65% of the apparently single fast-rotating stars are runaways. As a by-product, we discovered a new over-contact SB2 system (HD 165921) and two fast-rotating SB1 systems (HD 46485 and HD 152200) Also, we propose HD 94024 and HD 12323 (both SB1 systems with a v sin i < 200 km s−1) as candidates for hosting a quiescent stellar-mass black hole.
Conclusions. Our empirical results seem to be in good agreement with the assumption that the tail of fast-rotating O-type stars (with v sin i > 200 km s−1) is mostly populated by post-interaction binary products. In particular, we find that the final statistics of identified spectroscopic binaries and apparent single stars are in good agreement with newly computed predictions obtained with the binary population synthesis code BPASS and earlier estimations obtained in previous studies.