bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do Students Learn More with an Additional Teacher in the Classroom? Evidence from a Field Experiment
Authors: Haaland Venke Furre , Rege Mari , Solheim Oddny Judith 
Published: September 2023

Abstract
We present a field experiment investigating treatment effects of an additional teacher in the classroom on student learning. The treatment targets literacy instruction during first and second grades. Nearly 6,000 students in 300 classrooms participated in the experiment. The treatment has on average no effects on student learning. However, boys seem to benefit, with treatment impacts of about 0.12 and 0.09 standard deviations on the national literacy assessment and the reading self-concept, respectively. The effects seem to be particularly large for boys with low skills at baseline, with treatment impacts of 0.23 and 0.22 standard deviations.
Implementation and first outcomes of a novel standard operating procedure for preprocedural transoesophageal echocardiography screening in course of atrial arrhythmia ablation
Authors: Dittrich Sebastian , Kece Fehmi , Scheurlen Cornelia , van den Bruck Jan-Hendrik , Filipovic Karlo , Wörmann Jonas , Erlhöfer Susanne , Pavel Friederike , Schipper Jan-Hendrik , Sultan Arian , Lüker Jakob , Steven Daniel 
Published: September 2023

Abstract

Aims
Preprocedural transoesophageal echocardiography (TEE) screening for left atrial (LA) thrombi is the standard of care in many centres performing atrial fibrillation (AF) ablation. However, TEE imposes procedural risks for patients and is often challenging to implement in daily practice, besides causing patient discomfort. At our centre, a novel standard operating procedure (SOP) was implemented, aiming to identify patients that can be exempt from TEE screening. We aimed to assess whether this screening approach may reduce preprocedural TEEs without imposing patients of higher risks for cerebrovascular events (CVEs).

Methods and results
Data of 1874 consecutive patients treated by catheter ablation of LA arrhythmias between 2018 and 2022 were retrospectively analysed. A cohort of 937 patients, where decision to perform TEE screening was based on a new SOP (considering rhythm at admission, CHA2DS2-VASc score, and sufficient anticoagulation), was compared to a matched cohort receiving TEE before every procedure. Number of performed TEEs and incidences of CVEs were compared. Implementation of the new SOP led to a 67% reduction in TEEs performed (old SOP: 933 vs. new SOP: 305). No significant differences between the groups were detected regarding transitory ischaemic attack (old SOP: 5 vs. new SOP: 3; P = 0.48) and stroke (no events). No solid thrombi were detected during TEE screening.

Conclusion
The number of preprocedural screening TEEs before AF ablation procedures can be safely reduced by applying risk stratification based on rhythm at admission and CHA2DS2-VASc score, if anticoagulation was performed properly.
How to ablate the septo-pulmonary bundle: a case-based review of percutaneous ablation strategies to achieve roof line block
Authors: Tonko Johanna Bérénice , Silberbauer John , Mann Ian 
Published: September 2023

Abstract
Electrical conduction through cardiac muscle fibres separated from the main myocardial wall by layers of interposed adipose tissue are notoriously difficult to target by endocardial ablation alone. They are a recognised important cause for procedural failure due to the difficulties of delivering sufficient energy via the endocardial radiofrequency catheter to reach the outer epicardial layer without risking adverse events of the otherwise thin walled atria. Left atrial ablations for atrial fibrillation (AF) and tachycardia are commonly affected by the presence of several epicardial structures, with the septo-pulmonary bundle (SPB), Bachmann’s bundle, and the ligament of Marshall all posing substantial challenges for endocardial procedures. Delivery of a transmural lesion set is essential for sustained pulmonary vein isolation and for conduction block across linear atrial lines which in turn has been described to translate into a reduced AF/atrial tachycardia recurrence rate. To overcome the limitations of endocardial-only approaches, surgical ablation techniques for epicardial or combined hybrid endo-epicardial ablations have been described to successfully target these connections. Yet, these techniques confer an increase in procedure complexity, duration, cost, and morbidity. Alternatively, coronary venous system ethanol ablation has been successfully employed by sub-selecting the vein of Marshall to facilitate mitral isthmus line block, although this approach is naturally limited to this area by the coronary venous anatomy. Increased awareness of the pathophysiological relevance of these epicardial structures and their intracardiac conduction patterns in the era of high-resolution 3D electro-anatomical mapping technology has allowed greater understanding of their contribution to the persistence of AF as well as failure to achieve transmural block by traditional ablation approaches. This might translate into novel catheter ablation strategies with procedural success rates comparable to surgical ‘cut-and-sew’ techniques. This review aims to give an overview of percutaneous catheter ablation strategies to target the SPB, an important cause of failed block across the roof line and isolation of the left atrial posterior wall and/or the pulmonary veins. Existing and investigational technologies will be discussed and an outlook of future approaches provided.
Predicting Covid-19 infection and death rates among E.U. minority populations in the absence of racially disaggregated data through the use of US data comparisons
Authors: Cecchi Dimeglio Paola , Fullilove Robert E , Cecchi Catherine , Cabon Yann , Rosenberg Jessica 
Published: September 2023

Abstract

Background
The E.U.’s lack of racially disaggregated data impedes the formulation of effective interventions, and crises such as Covid-19 may continue to impact minorities more severely. Our predictive model offers insight into the disparate ways in which Covid-19 has likely impacted E.U. minorities and allows for the inference of differences in Covid-19 infection and death rates between E.U. minority and non-minority populations.

Methods
Data covering Covid-19, social determinants of health and minority status were included from 1 March 2020 to 28 February 2021. A systematic comparison of US and E.U. states enabled the projection of Covid-19 infection and death rates for minorities and non-minorities in E.U. states.

Results
The model predicted Covid-19 infection rates with 95–100% accuracy for 23 out of 28 E.U. states. Projections for Covid-19 infection and mortality rates among E.U. minority groups illustrate parallel trends to US rates.

Conclusions
Disparities in Covid-19 infection and death rates by minority status likely exist in patterns similar to those observed in US data. Policy Implications: Collecting data by race/ethnicity in the E.U. would help document health disparities and craft more targeted health interventions and mitigation strategies.
Identification of CryAB as a target of NUAK kinase activity in Drosophila muscle tissue
Authors: Zhao Ziwei , Brooks David , Guo Yungui , Geisbrecht Erika R 
Published: September 2023

Abstract
Phosphorylation reactions performed by protein kinases are one of the most studied post-translational modifications within cells. Much is understood about conserved residues within protein kinase domains that perform catalysis of the phosphotransfer reaction, yet the identity of the target substrates and downstream biological effects vary widely among cells, tissues, and organisms. Here, we characterize key residues essential for NUAK kinase activity in Drosophila melanogaster myogenesis and homeostasis. Creation of a NUAK kinase-dead mutation using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 results in lethality at the embryo to larval transition, while loss of NUAK catalytic function later in development produces aggregation of the chaperone protein αB-crystallin/CryAB in muscle tissue. Yeast 2-hybrid assays demonstrate a physical interaction between NUAK and CryAB. We further show that a phospho-mimetic version of NUAK promotes the phosphorylation of CryAB and this post-translational modification occurs at 2 previously unidentified phosphosites that are conserved in the primary sequence of human CryAB. Mutation of these serine residues in D. melanogaster NUAK abolishes CryAB phosphorylation, thus, proving their necessity at the biochemical level. These studies together highlight the importance of kinase activity regulation and provide a platform to further explore muscle tissue proteostasis.
The Impact of Hearing Impairment on Health Indicators in a Multiethnic Population of Older Adults in Singapore
Authors: Fenwick Eva K , Gupta Preeti , Chan Aurora W D , Man Ryan E K , Aravindhan Amudha , Ng Jia Hui , Cheng Ching-Yu , Wong Tien Y , Chan Angelique , Teo Neville Wei Yang , Sabanayagam Charumathi , Lamoureux Ecosse L 
Published: September 2023

Abstract

Background and Objectives
To determine the impact of hearing impairment (HI) on health indicators in a multiethnic Singaporean population of older adults.

Research Design and Methods
In this cross-sectional, population-based study, pure-tone averages of air-conduction thresholds at 500 Hz, 1,000 Hz, 2,000 Hz, and 4,000 Hz were calculated for each ear. Eight categories of HI were defined ranging from: 1: No HI to 8: Bilateral severe HI. Health indicators included hearing-related quality of life (H-QoL), depressive symptoms, frailty, gait speed, instrumental activities of daily living, sarcopenia, and cognitive impairment. Multivariable regression models determined the independent associations between HI and outcomes.

Results
A total of 2,503 older adults (mean age ± SD 73.4 ± 8.4; 55.2% female participants) were enrolled. Of these, 289 (11.6%), 259 (10.4%), 798 (31.9%), 303 (12.1%), 515 (20.6%), 52 (2.1%), 155 (6.2%), and 115 (4.6%) had hearing levels in Cats 1 to 8, respectively; and 20 (0.8%) used a hearing aid. Compared to those with no HI, participants with unilateral mild HI (Cat 2) had a 107% reduction in H-QoL (β: 0.63; CI: 0.18, 1.09, p = .006), increasing to a 2,816% reduction (β: 16.78; CI: 13.25, 20.31, p &lt; .001) in those with bilateral severe HI-Cat 8 (p-trend &lt; .001). Those with Cat 8 also had lower gait speed and we observed a nonsignificant increase in odds of frailty as HI worsened.

Discussion and Implications
H-QoL is affected across the spectrum of severity and laterality of HI. Interventions to alleviate the effects of HI and provision of QoL support are warranted. Other health indicators were only affected in late stages, suggesting that slowing disease progression is crucial in clinical management.
Tracing titanomagnetite alteration with magnetic measurements at cryogenic temperatures
Authors: Kosterov Andrei , Surovitskii Leonid , Maksimochkin Valerii , Yanson Svetlana , Smirnov Aleksey 
Published: September 2023

SUMMARY
Titanomagnetite containing up to 0.6–0.7 Ti atoms per formula unit is a primary magnetic mineral phase in submarine basalts and in some terrestrial volcanic rocks. On a geological timescale, it often undergoes alteration, forming new magnetic phases that may acquire (thermo)chemical remanent magnetization. The initial stage of this natural process can be modelled by prolonged laboratory annealing at moderately elevated temperatures. In this study, our goal is to characterize the alteration products resulting from annealing a submarine basalt containing homogeneous titanomagnetite Fe3−xTixO4 (x ≈ 0.46) at temperatures of 355, 500 and 550 °C for up to 375 hr, by examining their magnetic properties over a wide range of temperatures.
The effect of extended annealing is most apparent in the low-temperature magnetic properties. In the fresh sample, a magnetic transition is observed at 58 K. Below the transition temperature, the field-cooled (FC) and zero-field-cooled (ZFC) saturation isothermal remanent magnetization (SIRM) curves are separated by a tell-tale triangular-shaped area, characteristic for titanomagnetites of intermediate composition. The room-temperature SIRM (RT-SIRM) cycle to 1.8 K in zero field has a characteristic concave-up shape and is nearly reversible. For the annealed samples, the magnetic transition temperature shifts to lower temperatures, and the shape of the curves above the transition changes from concave-up to concave-down. The shape of the RT-SIRM cycles also progressively changes with increasing annealing time. The SIRM loss after the cycle increases up to ∼30 per cent for the samples annealed for 375 hr at 355 °C, and for 110 hr at 500 and 550 °C.
The Curie temperatures of the newly formed magnetic phases exceed the Curie temperature of the fresh sample (205 °C) by up to 350 °C. While this effect is most commonly attributed to extensive single-phase oxidation (maghemitization), the behaviour observed at cryogenic temperatures appears incompatible with the known properties of highly oxidized titanomaghemites. Therefore, we propose that, at least in the initial stage of the ‘dry’, that is, not involving hydrothermalism, alteration of titanomagnetite, temperature- and time-controlled cation reordering is the primary mechanism driving changes in both low- and high-temperature magnetic properties.
A common single nucleotide variant in the cytokine receptor-like factor-3 (CRLF3) gene causes neuronal deficits in human and mouse cells
Authors: Wilson Anna F , Barakat Rasha , Mu Rui , Karush Leah L , Gao Yunqing , Hartigan Kelly A , Chen Ji-Kang , Shu Hongjin , Turner Tychele N , Maloney Susan E , Mennerick Steven J , Gutmann David H , Anastasaki Corina 
Published: September 2023

Abstract
Single nucleotide variants in the general population are common genomic alterations, where the majority are presumed to be silent polymorphisms without known clinical significance. Using human induced pluripotent stem cell (hiPSC) cerebral organoid modeling of the 1.4 megabase Neurofibromatosis type 1 (NF1) deletion syndrome, we previously discovered that the cytokine receptor-like factor-3 (CRLF3) gene, which is co-deleted with the NF1 gene, functions as a major regulator of neuronal maturation. Moreover, children with NF1 and the CRLF3L389P variant have greater autism burden, suggesting that this gene might be important for neurologic function. To explore the functional consequences of this variant, we generated CRLF3L389P-mutant hiPSC lines and Crlf3L389P-mutant genetically engineered mice. While this variant does not impair protein expression, brain structure, or mouse behavior, CRLF3L389P-mutant human cerebral organoids and mouse brains exhibit impaired neuronal maturation and dendrite formation. In addition, Crlf3L389P-mutant mouse neurons have reduced dendrite lengths and branching, without any axonal deficits. Moreover, Crlf3L389P-mutant mouse hippocampal neurons have decreased firing rates and synaptic current amplitudes relative to wild type controls. Taken together, these findings establish the CRLF3L389P variant as functionally deleterious and suggest that it may be a neurodevelopmental disease modifier.
Cardiopulmonary bypass management and risk of new-onset atrial fibrillation after cardiac surgery
Authors: Taha Amar , Hjärpe Anders , Martinsson Andreas , Nielsen Susanne J , Barbu Mikael , Pivodic Aldina , Lannemyr Lukas , Bergfeldt Lennart , Jeppsson Anders 
Published: September 2023

Abstract

OBJECTIVES
Cardiopulmonary bypass (CPB) management may potentially play a role in the development of new-onset atrial fibrillation (AF) after cardiac surgery. The aim of this study was to explore this potential association.

METHODS
Patients who underwent coronary artery bypass grafting and/or valvular surgery during 2016–2020 were included in an observational single-centre study. Data collected from the Swedish Web System for Enhancement and Development of Evidence-Based Care in Heart Disease Evaluated According to Recommended Therapies registry and a local CPB database were merged. Associations between individual CPB variables (CPB and aortic clamp times, arterial and central venous pressure, mixed venous oxygen saturation, blood flow index, bladder temperature and haematocrit) and new-onset AF were analysed using multivariable logistic regression models adjusted for patient characteristics, comorbidities and surgical procedure.

RESULTS
Out of 1999 patients, 758 (37.9%) developed new-onset AF. Patients with new-onset postoperative AF were older, had a higher incidence of previous stroke, worse renal function and higher EuroSCORE II and CHA2DS2-VASc scores and more often underwent valve surgery. Longer CPB time [adjusted odds ratio 1.05 per 10 min (95% confidence interval 1.01–1.08); P = 0.008] and higher flow index [adjusted odds ratio 1.21 per 0.2 l/m2 (95% confidence interval 1.02–1.42); P = 0.026] were associated with an increased risk for new-onset AF, while the other variables were not. A sensitivity analysis only including patients with isolated coronary artery bypass grafting supported the primary analyses.

CONCLUSIONS
CPB management following current guideline recommendations appears to have minor or no influence on the risk of developing new-onset AF after cardiac surgery.
Group sparse optimization for inpainting of random fields on the sphere
Authors: Li Chao , Chen Xiaojun 
Published: September 2023

Abstract
We propose a group sparse optimization model for inpainting of a square-integrable isotropic random field on the unit sphere, where the field is represented by spherical harmonics with random complex coefficients. In the proposed optimization model, the variable is an infinite-dimensional complex vector and the objective function is a real-valued function defined by a hybrid of the $\ell _2$ norm and non-Lipschitz $\ell _p (0&lt;p&lt;1)$ norm that preserves rotational invariance property and group structure of the random complex coefficients. We show that the infinite-dimensional optimization problem is equivalent to a convexly-constrained finite-dimensional optimization problem. Moreover, we propose a smoothing penalty algorithm to solve the finite-dimensional problem via unconstrained optimization problems. We provide an approximation error bound of the inpainted random field defined by a scaled Karush–Kuhn–Tucker (KKT) point of the constrained optimization problem in the square-integrable space on the sphere with probability measure. Finally, we conduct numerical experiments on band-limited random fields on the sphere and images from Cosmic Microwave Background (CMB) data to show the promising performance of the smoothing penalty algorithm for inpainting of random fields on the sphere.
Post-report antibiotic initiation following community non-sterile-site microbiology results: an opportunity for labs to lead stewardship?
Authors: Bloomfield Max , Todd Sue , van der Werff Koen , Blackmore Tim , Balm Michelle 
Published: September 2023

Abstract

Objectives
Positive culture results from non-sterile sites (NSSs) are poorly predictive of clinical infection. Despite this, these results are often interpreted as an indication for antibiotics, even in patients with limited signs of infection. We sought to quantify the influence of NSS culture results on post-report antibiotic initiation (PRAI) in patients who had not been started on antibiotics pre-report.

Methods
All community wound/skin swab and sputum cultures were matched to antibiotic dispensing records from February 2017 to July 2022. Prescribing behaviour was assessed pre- and post-report. Sampling without treatment pre-report was termed ‘test-and-wait’ (TaW). Following TaW, PRAI was identified if antibiotics were started within 5 days post-report.

Results
There were 65 480 wound/skin swabs and 8126 sputum samples, with TaW occurring in 21 740 (35.1%) and 4185 (54.4%), respectively. Following a TaW approach PRAI occurred in 43.3% when an organism was reported, versus 10.8% (P &lt; 0.01) for a ‘no growth’ report for wound/skin swabs. For the same comparison with sputum, PRAI occurred in 47.9% versus 10.8% (P &lt; 0.01). On multivariate analysis reporting an organism remained strongly associated with PRAI.

Conclusions
Reporting an organism in those not already on antibiotics was strongly associated with PRAI. We hypothesize that for many patients TaW suggests limited evidence of infection (i.e. insufficient to justify antibiotic treatment at time of sampling), meaning positive NSS results may be driving a considerable volume of potentially unnecessary antibiotic use. Further study on this topic is required, but strategies to reduce PRAI may offer laboratories an opportunity to meaningfully impact antimicrobial stewardship efforts.
Scale of analysis drives the observed ratio of spatial to non-spatial variance in microbial water quality: insights from two decades of citizen science data
Authors: Weller Daniel L , Love Tanzy M T , Weller Donald E , Murphy Claire M , Strawn Laura K 
Published: September 2023

Abstract

Aims
While fecal indicator bacteria (FIB) testing is used to monitor surface water for potential health hazards, observed variation in FIB levels may depend on the scale of analysis (SOA). Two decades of citizen science data, coupled with random effects models, were used to quantify the variance in FIB levels attributable to spatial versus temporal factors.

Methods and results
Separately, Bayesian models were used to quantify the ratio of spatial to non-spatial variance in FIB levels and identify associations between environmental factors and FIB levels. Separate analyses were performed for three SOA: waterway, watershed, and statewide. As SOA increased (from waterway to watershed to statewide models), variance attributable to spatial sources generally increased and variance attributable to temporal sources generally decreased. While relationships between FIB levels and environmental factors, such as flow conditions (base versus stormflow), were constant across SOA, the effect of land cover was highly dependent on SOA and consistently smaller than the effect of stormwater infrastructure (e.g. outfalls).

Conclusions
This study demonstrates the importance of SOA when developing water quality monitoring programs or designing future studies to inform water management.
G protein-coupled bile acid receptor 1 reduced hepatic immune response and inhibited NFκB, PI3K/AKT, and PKC/P38 MAPK signaling pathway in hybrid grouper
Authors: Xu Jia , Cao Junming , Tan Beiping , Xie Shiwei 
Published: September 2023

Abstract
The mammalian G protein-coupled bile acid receptor 1 (TGR5) is involved in the inflammatory response. However, the functions of TGR5 in the immune response of fish remain unclear. In this study, the full-length sequence of tgr5 from hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) was cloned, and the function of TGR5 in the immune response was explored. The results showed that the ORF of tgr5 gene in hybrid grouper was 1029 bp and encoded 342 amino acids. Activation of TGR5 by INT-777 significantly decreased the activities and mRNA expression of TNFα and IL1β, whereas inhibition of TGR5 by SBI-115 showed the opposite effect. SBI-115 treatment significantly increased the expression of phosphorylated inhibitor κB α (p-IKBα) protein. After the INT-777 treatment, the concentration of protein kinase C (PKC) and expression of the p38 mitogen-activated protein kinases (p38a), p38b and p38c, were significantly decreased in vivo. INT-777 agonist significantly decreased the expression of phosphorylated phosphoinositide 3-kinase (p-PI3K) protein and the ratio of phosphorylated and nonphosphorylated serine/threonine-protein kinase (p-AKT/AKT). In conclusion, activation of hepatic TGR5 inhibited the PKC/P38 MAPK, PI3K/AKT, NFκB signaling pathway and improved hepatic immune responses of hybrid grouper in vivo and in vitro.