bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sunlight photolysis of SARS-CoV-2 N1 gene target in the water environment: considerations for the environmental surveillance of wastewater-impacted surface waters
Authors: Dunn Fiona B. , Silverman Andrea I. 
Published: August 2023

Abstract

Wastewater surveillance of SARS-CoV-2 has been used around the world to supplement clinical testing data for situational awareness of COVID-19 disease trends. Many regions of the world lack centralized wastewater collection and treatment infrastructure, which presents additional considerations for wastewater surveillance of SARS-CoV-2, including environmental decay of the RT-qPCR gene targets used for quantification of SARS-CoV-2 virions. Given the role of sunlight in the environmental decay of RNA, we evaluated sunlight photolysis kinetics of the N1 gene target in heat-inactivated SARS-CoV-2 with a solar simulator under laboratory conditions. Insignificant photolysis of the N1 target was observed in a photosensitizer-free matrix. Conversely, significant decay of the N1 target was observed in wastewater at a shallow depth (&lt;1 cm). Given that sunlight irradiance is affected by several environmental factors, first-order decay rate models were used to evaluate the effect of water column depth, time of the year, and latitude on decay kinetics. Decay rate constants were found to decrease significantly with greater depth of the well-mixed water column, at high latitudes, and in the winter. Therefore, sunlight-mediated decay of the N1 gene target is likely to be minimal, and is unlikely to confound results from wastewater-based epidemiology programs utilizing wastewater-impacted surface waters.
TRIM65 Suppresses oxLDL-induced Endothelial Inflammation by
Interaction with VCAM-1 in Atherogenesis
Authors: Ma Xiao-Feng , Zhou Yi-Ren , Zhou Zhi-Xiang , Liu Hui-Ting , Zhou Bo-Bin , Deng Nian-Hua , Zhou Kun , Tian Zhen , Wu Ze-Fan , Liu Xi-Yan , Fu Ming-Gui , Jiang Zhi-Sheng 
Published: August 2023

Background and Objective:
Endothelial cell activation, characterized by increased
levels of vascular cell adhesion molecule 1 (VCAM-1), plays a crucial role in the
development of atherosclerosis (AS). Therefore, inhibition of VCAM-1-mediated inflammatory
response is of great significance in the prevention and treatment of AS. The tripartite
motif (TRIM) protein-TRIM65 is involved in the regulation of cancer development,
antivirals and inflammation. We aimed to study the functions of TRIM65 in regulating
endothelial inflammation by interacting with VCAM-1 in atherogenesis.

Methods and Results:
In vitro, we report that human umbilical vein endothelial cells
(HUVECs) treated with oxidized low-density lipoprotein (oxLDL) significantly upregulate
the expression of TRIM65 in a time- and dose-dependent manner. Overexpression of
TRIM65 reduces oxLDL-triggered VCAM-1 protein expression, decreases monocyte adhesion
to HUVECs and inhibits the production of the inflammatory cytokines IL-1β,
IL-6, IL-8, and TNF-α as well as endothelial oxLDL transcytosis. In contrast, siRNA-mediated
knockdown of TRIM65 promotes the expression of VCAM-1, resulting in increased
adhesion of monocytes and the release of the inflammatory cytokines IL-1β,
IL-6, IL-8, and TNF-α and enhances endothelial oxLDL transcytosis. In vivo, we measured
the high expression of TRIM65 in ApoE-/- mouse aortic plaques compared to
C57BL/6J mouse aortic plaques. Then, we examined whether the blood levels of
VCAM-1 were higher in TRIM65 knockout ApoE-/- mice than in control mice induced by
a Western diet. Furthermore, Western blot results showed that the protein expression of
VCAM-1 was markedly enhanced in TRIM65 knockout ApoE-/- mouse aortic tissues compared
to that of the controls. Immunofluorescence staining revealed that the expression
of VCAM-1 was significantly increased in atherosclerotic plaques of TRIM65-/-/ApoE-/-
aortic vessels compared to ApoE-/- controls. Mechanistically, TRIM65 specifically interacts
with VCAM-1 and targets it for K48-linked ubiquitination.

Conclusion:
Our studies indicate that TRIM65 attenuates the endothelial inflammatory
response by targeting VCAM-1 for ubiquitination and provides a potential therapeutic target
for the inhibition of endothelial inflammation in AS.
Advances on the Role of Ferroptosis in Ionizing Radiation Response
Authors: Wang Fang , Dai QingHui , Xu Luhan , Gan Lu , Shi Yidi , Yang Mingjun , Yang Shuhong 
Published: August 2023

Abstract:
Ferroptosis is an iron-dependent programmed cell death mode that is distinct from other cell death modes, and radiation is able to stimulate cellular oxidative stress and induce the production of large amounts of reactive oxygen radicals, which in turn leads to the accumulation of lipid peroxide and the onset of ferroptosis. In this review, from the perspective of the role of ferroptosis in generating a radiation response following cellular irradiation, the relationship between ferroptosis induced by ionizing radiation stress and the response to ionizing radiation is reviewed, including the roles of MAPK and Nrf2 signaling pathways in ferroptosis, resulting from the oxidative stress response to ionizing radiation, the metabolic regulatory role of the p53 gene in ferroptosis, and regulatory modes of action of iron metabolism and iron metabolism-related regulatory proteins in promoting and inhibiting ferroptosis. It provides some ideas for the follow-up research to explore the specific mechanism and regulatory network of ferroptosis in response to ionizing radiation.
Lentiviral Transduction-based CRISPR/Cas9 Editing of Schistosoma
mansoni Acetylcholinesterase
Authors: Du Xiaofeng , McManus Donald P. , French Juliet D. , Sivakumaran Haran , Johnston Rebecca L. , Kondrashova Olga , Fogarty Conor E. , Jones Malcolm K. , You Hong 
Published: August 2023

Background:
Recent studies on CRISPR/Cas9-mediated gene editing in Schistosoma mansoni
have shed new light on the study and control of this parasitic helminth. However, the gene editing
efficiency in this parasite is modest.

Methods:
To improve the efficiency of CRISPR/Cas9 genome editing in schistosomes, we used lentivirus,
which has been effectively used for gene editing in mammalian cells, to deliver plasmid DNA
encoding Cas9 nuclease, a sgRNA targeting acetylcholinesterase (SmAChE) and a mCherry fluorescence
marker into schistosomes.

Results:
MCherry fluorescence was observed in transduced eggs, schistosomula, and adult worms, indicating
that the CRISPR components had been delivered into these parasite stages by lentivirus. In
addition, clearly changed phenotypes were observed in SmAChE-edited parasites, including decreased
SmAChE activity, reduced hatching ability of edited eggs, and altered behavior of miracidia hatched
from edited eggs. Next-generation sequencing analysis demonstrated that the lentiviral transductionbased
CRISPR/Cas9 gene modifications in SmAChE-edited schistosomes were homology-directed repair
predominant but with much lower efficiency than that obtained using electroporation (data previously
published by our laboratory) for the delivery of CRISPR components.

Conclusion:
Taken together, electroporation is more efficient than lentiviral transduction in the delivery
of CRISPR/Cas9 into schistosomes for programmed genome editing. The exploration of tactics for
enhancing CRISPR/Cas9 gene editing provides the basis for the future improvement of programmed
genome editing in S. mansoni.
Rational Design and Synthesis of Novel Benzimidazole Derivatives as Potential β-Glucosidase Inhibitors
Authors: Liu Xu , Sun Ge , Li Fengxing , Feng Xia , Jia Tongguan , Luo Cheng , Chen Shijie , Chen Hua 
Published: August 2023

Background:
β-Glucosidase has a variety of biological functions. A structural model for a potential β-glucosidase inhibitor has been proposed in the previous studies.

Objective:
A series f new diaryl derivatives linked through benzimidazole have been randomly and rationally designed, synthesized, and evaluated for their inhibitory activities against β-glucosidase (almond). The proposed structural model provides a new strategy for the design of potent β-glucosidase inhibitors.

Methods:
According to the model, a series of benzimidazole derivatives were designed and synthesized, and their inhibitory activity, Ki value, inhibitory type, and binding mode analysis (PDB ID: 2J7C) on β-glucosidase (almond) were evaluated.

Results:
Two compounds 7b and 7d were the best inhibitors with IC50 values of 7.86 M and 3.52 μM, respectively. Their Ki values were calculated to be 9.91 μM and 5.81 μM, respectively.

Conclusion:
The SAR analysis suggested that such benzimidazole derivatives might bind to the active site of β-glucosidase mainly through hydrogen bonds on o-trihydroxyphenol; the additional phenyl ring on the other side towards the solvent-exposed region played a very important role in improving their inhibitory activity against β-glucosidase
Gut-Gonad Perturbations in Type-1 Diabetes Mellitus: Role of Dysbiosis,
Oxidative Stress, Inflammation and Energy-Dysbalance
Authors: Panghal Archna , Jena Gopabandhu 
Published: August 2023

Abstract:
Type 1 diabetes mellitus is a major metabolic disorder that affects people of all age
groups throughout the world. It is responsible for the alterations in male gonadal physiology in experimental
models as well as in clinical cases. On the other side, diabetes mellitus has also been associated
with perturbations in the gut physiology and microbiota dysbiosis. The accumulating evidence
suggests a link between the gut and gonad as evident from the i) experimental data providing
insights into type 1 diabetes mellitus induced gut perturbations, ii) link of gut physiology with alterations
of testicular health, iii) role of gut microbiota in androgen metabolism in the intestine, and
iv) epidemiological evidence linking type 1 diabetes mellitus with inflammatory bowel disease and
male infertility. Considering all the pieces of evidence, it is summarized that gut dysbiosis, oxidative
stress, inflammation and energy dys-balance are the prime factors involved in the gonadal
damage under type 1 diabetes mellitus, in which the gut contributes significantly. Identification of
novel biomarkers and intervention of suitable agents targeting these prime factors may be a step
forward to restore the gonadal damage in diabetic conditions.
Maternal Dietary Intakes and Risk of Spontaneous Abortion: A Systematic Review
Authors: Amini Shirin , Sheykhon Ayla Alizadeh , Abiri Behnaz , Jafarirad Sima 
Published: August 2023

Background:
The loss of a fetus before the 20th week of pregnancy is known as
spontaneous abortion (SA).

Objective:
This systematic review aimed to evaluate the results of previous studies about the dietary
intake of mothers who suffered from SA.

Methods:
We conducted a comprehensive search in the Web of Science, Cochrane, PubMed,
ProQuest, Google Scholar, Science Direct, and Scopus databases to collect relevant published
studies between 1990 and November 2022. The data from case-control and cohort studies that had
assessed dietary intakes before and during pregnancy of women with SA were included.

Results:
High consumption of butter increases the risk of SA, but a very low intake of olive oil and
omega-3 fatty acids can reduce the risk of SA. In addition, an adequate intake of high-quality proteins
and calcium from dairy products and enough intake of fiber and phytochemicals from
chocolate, fresh fruits, and vegetables can reduce the risk of SA. A number of mechanisms, such as
providing essential nutrients for fertilization and implantation, promoting fetal growth, reduction of
inflammatory conditions, and insulin resistance, have been proposed to explain the protective
effects of these food groups against SA.

Conclusion:
An unhealthy diet and eating sufficient amounts of these food groups will be helpful
and recommended.
Antibacterial Evaluation of Gallic Acid and its Derivatives against a Panel
of Multi-drug Resistant Bacteria
Authors: Abdella Mohamed , Lahiri Chandrajit , Abdullah Iskandar , Anwar Ayaz 
Published: August 2023

Background:
Infectious diseases are the second leading cause of deaths worldwide. Pathogenic
bacteria have been developing tremendous resistance against antibiotics which has placed an
additional burden on healthcare systems. Gallic acid belongs to a naturally occurring phenolic class
of compounds and is known to possess a wide spectrum of antimicrobial activities.

Aims < Objectives:
In this study, we synthesized thirteen derivatives of gallic acid and evaluated
their antibacterial potential against seven multi-drug resistant bacteria, as well as cytotoxic effects
against human embryonic kidney cell line in vitro..

Methods:
13 compounds were successfully synthesized with moderate to good yield and evaluated.
Synthesized derivatives were characterized by using nuclear magnetic resonance spectroscopy, mass
spectrometry, and Fourier transformation infrared spectroscopy. Antibacterial activity was determined
using microdilution while cytotoxicyt was assessed using MTT assay.

Results:
The results of antibacterial assay showed that seven out of thirteen compounds exhibited
antibacterial effects with compound 6 and 13 being most potent against Staphylococcus aureus (MIC
56 μg/mL) and Salmonella enterica (MIC 475 μg/mL) respectively. On the other hand, most of these
compounds showed lower cytotoxicity against human embryonic kidney cells (HEK 293), with IC50
values ranging from over 700 μg/mL

Conclusion:
Notably, compound 13 was found to be non-toxic at concentrations as high as 5000
μg/mL. These findings suggest that the present synthetic derivatives of gallic acid hold potential for
further studies in the development of potent antibacterial agents.
Protective Effect of Heteroxenia fuscescens Extract against Sodium
Arsenite-induced Infertility in Male Rats
Authors: Omar Amel , Soliman Basma , Dakrory Ahmed , Al-Hammady Montaser , Mohamed Ayman 
Published: August 2023

Background:
Arsenic is a potent environmental toxin with dangerous effects on human
and animal populations. Heteroxenia fuscescens (H. fuscescens) extract exhibits potential health
effects. The purpose of this study was to assess the protective effect of H. fuscescensextract against
sodium arsenite-induced infertility and toxicity in rats.

Methods:
Forty male rats were separated into four groups as follows: control group (2% DMSO,
orally), sodium arsenite (10 mg/kg, orally), sodium arsenite + H. fuscescens extract (60 mg/kg in
2% DMSO), and H. fuscescens extract (60 mg/kg in 2% DMSO).

Results:
GC/MS analysis of H. fuscescens indicates the presence of 10 compounds at retention
times of 6.00, 12.53, 19.04, 24.60, 28.56, 35.66, 38.99, 45.29, 48.02, and 52.14. fuscescens extract
improves levels of sperm count (53.33 ± 1.52), motility (43.00 ± 1.10), FSH ( 2.17 ± 0.04), LH
(2.43 ± 0.06), testosterone (1.87 ± 0.02), and testis weight (0.49 ± 0.01). The antioxidant activity of
H. fuscescens extract was reported by a significant decrease in MDA (1.02 ± 0.01) and NO (520.39
± 14.99) levels, while it significantly increased levels of GSH (1.77 ± 0.07), and CAT (71.38 ±
3.45). Histopathological alterations of the testes, liver, and kidney observed with sodium arsenite
have been improved in the treatment group.

Conclusion:
Heteroxenia fuscescens is beneficial in restoring male sex hormone levels, maintaining
a healthy sperm profile, and reducing oxidative stress, all of which lead to an improvement in
male rat fertility.
Banxia Xiexin Decoction Prevents HT22 Cells from High Glucose-induced Neurotoxicity via JNK/SIRT1/Foxo3a Signaling Pathway
Authors: Shi Yinli , Sheng Pei , Guo Ming , Chen Kai , Zhao Yun , Wang Xu , Wu Mianhua , Li Bo 
Published: August 2023

Background:
Type 2 diabetes-associated cognitive dysfunction (DCD) is a chronic
complication of diabetes that has gained international attention. The medicinal compound Banxia
Xiexin Decoction (BXXXD) from traditional Chinese medicine (TCM) has shown potential in
improving insulin resistance, regulating endoplasmic reticulum stress (ERS), and inhibiting cell
apoptosis through various pathways. However, the specific mechanism of action and medical value
of BXXXD remain unclear.

Methods:
We utilized TCMSP databases to screen the chemical constituents of BXXXD and
identified DCD disease targets through relevant databases. By using Stitch and String databases,
we imported the data into Cytoscape 3.8.0 software to construct a protein-protein interaction (PPI)
network and subsequently identified core targets through network topology analysis. The core
targets were subjected to Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of
Genes and Genomes (KEGG) pathway analyses. The results were further validated through in
vitro experiments.

Results:
Network pharmacology analysis revealed the screening of 1490 DCD-related targets and
190 agents present in BXXXD. The topological analysis and enrichment analysis conducted using
Cytoscape software identified 34 core targets. Additionally, GO and KEGG pathway analyses
yielded 104 biological targets and 97 pathways, respectively. BXXXD exhibited its potential in
treating DCD by controlling synaptic plasticity and conduction, suppressing apoptosis, reducing
inflammation, and acting as an antioxidant. In a high glucose (HG) environment, the expression of
JNK, Foxo3a, SIRT1, ATG7, Lamp2, and LC3 was downregulated. BXXXD intervention on
HT22 cells potentially involved inhibiting excessive oxidative stress, promoting neuronal autophagy,
and increasing the expression levels of JNK, SIRT1, Foxo3a, ATG7, Lamp2, and LC3. Furthermore,
the neuroprotective effect of BXXXD was partially blocked by SP600125, while quercetin
enhanced the favorable role of BXXXD in the HG environment.

Conclusion:
BXXXD exerts its effects on DCD through multiple components, targets, levels, and
pathways. It modulates the JNK/SIRT1/Foxo3a signaling pathway to mitigate autophagy inhibition
and apoptotic damage in HT22 cells induced by HG. These findings provide valuable perspectives
and concepts for future clinical trials and fundamental research.
Development and Validation of HPLC-UV Method for the Determination
of Levothyroxine in Orodispersible Minitablets for Pediatric Application
Authors: Belen Olivera Camila , Cecilia Henestrosa , Silvina Favier , Cecilia Dobrecky , Sabrina Flor , Silvia Lucangioli 
Published: August 2023

Background:
Thyroid hormones play an important role in cognition and brain development.
The determination of the content of levothyroxine, as well as related substances and dissolution test analysis,
should be carried out by methods that are selective and highly sensitive due to the low concentration
used in low dose orodispersible minitablets

Objectives:
This study aims to develop and validate an analytical method by HPLC-UV for the quantification
of levothyroxine and its related substances in pharmaceutical formulations; looking forward to
being this method suitable for a future dissolution test analysis using tandem mass spectrometry detector.

Methods:
A Hypersil GOLD C18 (100 x 2.1 mm, 3 μm) column was used with 25°C column temperature,
5 μL injection volume, 0.3 mL/min flow rate and detection at 225 nm. The mobile phase consisted
of methanol: 0.05% formic acid (55:45). The developed method was validated for specificity, linearity,
precision, accuracy and robustness.

Results:
The method is linear within the range of 2-20 μg mL-1 (R2=0.9982), which makes the method
suitable for the evaluation of levothyroxine in pharmaceuticals formulations. LOQ was 0.17 μg/mL (0.85
ng on column) and LOD 0.05 μg/mL (0.25 ng on column) of LT4. Therefore, in terms of efficiency
(1671), retention factor, k (6.79), Tailing factor, T (1.09) and resolution, Rs (5.11) the chromatographic
method was found to be suitable according to USP 43.

Conclusion:
The HPLC UV method was found to be linear, specific, precise, accurate and robust, therefore
it is suitable for the quality control of levothyroxine in pharmaceutical ODMTs..
Determination of Sufentanil in Human Plasma Using Ultra-high
Performance Liquid Chromatography Coupled with Tandem Mass
Spectrometry (UPLC–MS/MS)
Authors: Lee Sang-Min , Kim Suin , Shin Dongseong , Shin Kwang-Hee 
Published: August 2023

Background:
Sufentanil, an opioid analgesic, is used as an induction agent for general anesthesia
during surgery. Sufentanil is more active than other anesthetics and has a narrow therapeutic range.
Therefore, a precise dosage regimen is necessary when administering sufentanil.

Objective:
This study aimed to develop a bioanalytical method for the determination of sufentanil within
the sensitive concentration range more than that in a previous study utilizing protein precipitation (PP)
and determine the plasma concentration of sufentanil.

Methods:
A method for quantitating sufentanil was developed using ultra–high performance liquid chromatography
coupled with mass spectrometry (UPLC–MS/MS) and detection by electrospray ionization
(ESI). The internal standard was sufentanil-d5. Chromatographic separation was performed using an
Acquity UPLC HSS T3 column (50 × 2.1 mm, 1.8 μm) from Waters (Milford, MA, USA). Protein precipitation
(PP) was used for sample preparation, and gradient elution was conducted using a mobile phase
consisting of 1 mL of 2M ammonium acetate in 99% formic acid in 1 L of water or in 1 L of acetonitrile.
The total run time for the analysis was 5 min, and the flow rate was 0.4 mL/min.

Results:
Standard curves were linear over ranges of 0.025–30 ng/mL for sufentanil with a correlation
coefficient (r2) greater than 0.9998. The lower limit of quantification (LLOQ) was 0.025 ng/mL. The
intra– and inter–day accuracies were 97.66%–108.8% and 101.25%–103.17%, respectively, and the precision
did not exceed 15% for sufentanil.

Conclusion:
This study developed a validated, simple, sensitive, and convenient UPLC–MS/MS method
for quantifying sufentanil in human plasma. This method was applied to determine sufentanil concentration
in the plasma of patients undergoing surgery. The bioanalytical method using PP showed a sufficiently
sensitive concentration range. This method could be applied to various pharmacokinetic studies of
sufentanil.
Research Progress on Cardiac Tissue Construction of Mesenchymal Stem
Cells for Myocardial Infarction
Authors: Yang Guo-Dong , Ma Da-Shi , Ma Chun-Ye , Bai Yang 
Published: August 2023

Abstract:
Heart failure is still the main complication affecting the prognosis of acute myocardial infarction
(AMI), and mesenchymal stem cells (MSCs) are an effective treatment to replace necrotic myocardium
and improve cardiac functioning. However, the transplant survival rate of MSCs still presents challenges.
In this review, the biological characteristics of MSCs, the progress of mechanism research in the
treatment of myocardial infarction, and the advances in improving the transplant survival rate of MSCs in
the replacement of necrotic myocardial infarction are systematically described. From a basic to advanced
clinical research, MSC transplants have evolved from a pure injection, an exosome injection, the genetic
modification of MSCs prior to injection to the cardiac tissue engineering of MSC patch grafting. This
study shows that MSCs have wide clinical applications in the treatment of AMI, suggesting improved
myocardial tissue creation. A broader clinical application prospect will be explored and developed to
improve the survival rate of MSC transplants and myocardial vascularization.