bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterologous prime-boost regimen elicits potent humoral and cell-mediated immune responses and provides complete protection against SFTSV
Authors: Kim Jae-Yong , Jeon Kyeongseok , Hong Jung Joo , Park Sang-In , Cho Hyeong-Gon , Park Hyo-Jung , Kwak Hye Won , Park Hyeong-Jun , Bang Yoo-Jin , Lee Yu-Sun , Bae Seo-Hyeon , Kim So-Hee , Hwang Kyung-Ah , Jung Dae-Im , Cho Seong Hoo , Seo Sang Hwan , Kim Green , Oh Hanseul , Lee Hwal-Yong , Kim Ki Hyun , Lim Hee-Young , Jeon Pyeonghwa , Lee Joo-Yeon , Chung Junho , Lee Sang-Myeong , Ko Hae Li , Song Manki , Cho Nam-Hyuk , Lee Young-suk , Hong So-Hee , Nam Jae-Hwan 
Published: January 2023

Abstract
Severe Fever with Thrombocytopenia Syndrome Virus(SFTSV) was first discovered in 2009 as the causative agent of severe fever with thrombocytopenia syndrome. Despite its potential threat to public health, no prophylactic vaccine is yet available. This study developed a heterologous prime-boost strategy comprising priming with recombinant replication-deficient human adenovirus type 5 (rAd5) expressing the surface glycoprotein, Gn, and boosting with Gn protein. This vaccination regimen induced balanced Th1/Th2 immune responses and resulted in potent humoral and T cell-mediated responses in mice. It elicited high neutralizing antibody titers in both mice and non-human primates. Transcriptome analysis revealed that rAd5 and Gn proteins induced adaptive and innate immune pathways, respectively. This study provides immunological and mechanistic insight into this heterologous regimen and paves the way for future strategies against emerging infectious diseases.
Side-to-side, end-to-end, embedded anastomosis versus end-to-side anastomosis in patients with esophageal carcinoma：A retrospective, controlled study
Authors: Zhou Yun , Li Tiantian , Yang Zhiliang , Tan Zhiming , Zheng Lunhui , Yang Chifen , Zhang Chunguang , Pan Run , Ding Yao , Guo Lei , Chen Tao , Wang Zheng 
Published: January 2023

Abstract
Background
Anastomotic leakage is one of the most serious postoperative complications of esophageal carcinoma. A new method of anastomosis has been adopted to reduce the incidence of anastomotic leakage. The aim of this study was to verify the safety and effectiveness of this new anastomosis method by comparing with the traditional anastomosis method.
Methods
A total of 121 patients with esophageal cancer from January 2020 to January 2022 in our department were included. They were divided into two groups according to the different methods of esophagogastrostomy: Side-to-side, end-to-end, embedded anastomosis group (SEE group, n=68) and traditional end-to-side anastomosis group (TE group, n=69). On the 7th day after operation, esophagography was performed to confirm the existence of anastomotic leakage, dysphagia, anastomotic stenosis, and gastroesophageal reflux.
Results
The incidence of anastomotic leakage in TE group and SEE group were 14.5% and 3.4%, respectively, with statistical significance(p＜0.05). The incidence of anastomotic stenosis in TE group was 21%, while in SEE group it was 3.4%. These differences were statistically significant(p＜0.01). The incidence of reflux in the TE group and the SEE group was 24.2% and 8.5%, respectively, with statistical significance(p＜0.05). The first esophagography after operation showed that the incidence of swallowing disorder was 27.4% in TE group and 57.6% in SEE group. These differences were statistically significant(p＜0.01). However, the incidence of swallowing disorder in TE group and SEE group was 9.7% and 15.3%, respectively, with no significant difference after 3 months (p＞0.05).
Conclusion
SEE anastomosis is a safe and effective surgical method, which is worthy of clinical application.
Effects of allyl isothiocyanate fumigation on medicinal plant root knot disease control, plant survival, and the soil bacterial community
Authors: Li Yingbin , Lu Daqing , Xia Yan , Xu Xinjing , Huang Huichuan , Mei Xinyue , Yang Min , Li Jianqiang , Zhu Shusheng , Liu Yixiang , Zhang Zhiping 
Published: January 2023

Abstract
Background
Allyl isothiocyanate (AITC) is a natural product with high volatility that is used as a biofumigant to alleviate soil-borne plant diseases, and problems such as root knot nematodes (RKNs) that necessitate continuous cropping. However, little research has assessed the effects of AITC fumigation on medicinal plant.
Results
The current results indicated that AITC significantly reduced the population of RKNs in soil (p < 0.0001) and showed an excellent RKN disease control effect within 6 months after sowing Panax notoginseng (p < 0.0001). The seedling survival rate of 2-year-old P. notoginseng was approximately 1.7-fold higher after soil treatment with AITC (p = 0.1008). 16S rRNA sequencing indicated that the AITC treatment affected bacterial richness rather than diversity in the CC soil. Furthermore, biomarkers with statistical differences between AITC-treated and untreated CC soil showed that Pirellulales (o), Pirellulaceae (f), Pseudomonadaceae (f), and Pseudomonas (g) played important roles in the AITC-treated group. In addition, the microbiome functional phenotypes predicted using the BugBase tool suggested that AITC treatment is more conducive to improving CC soil through changes in the bacterial community structure. Crucially, our research confirmed that AITC soil treatment significantly increases soil organic matter (p = 0.0055), total nitrogen (p = 0.0054), and available potassium (p = 0.0373), which promotes the succeeding medicinal plant (Polygonatum kingianum) survival.
Conclusion
AITC as an ecologically friendly soil treatment method, which does not affect soil microbial diversity, but rather relative abundance. Also, it could be providing a basis for a useful agricultural soil management measure to alleviate soil sickness.
Alternative Chemicals as Source of Nitrogen to Enhance Ginger(zingiber officinal Rosc.) In vitro Micro-propagation in Ethiopia
Authors: Gezahegn Genene Konobo , Feyissa Tileye Feyissa , Rezene Yayis Tedla 
Published: January 2023

Abstract
Ginger is used for many purposes and become a major spice crop across the country in Ethiopia. Production was challenged primarily due to bacterial wilt disease eruption as of the 2012 production season. The use of disease-free tissue culture generated seed rhizome as part of integrated management was considered as the best option to reduce the disease impact. To enhance disease-free ginger in vitro propagation; an experiment was designed to select alternative sources of nitrogen as replacement of ammonium nitrate. Three alternative salts at different levels were tasted as potential replacements of ammonium nitrate in MS medium supplemented with 2.0 mg/l BAP and 1.0 mg/l Kinetin using Boziab variety. The highest mean shoot number (9.33) was achieved from 1.0 g/l NH4Cl followed by 3.8 g/l KNO3 and 3 g/l urea with a record of mean shoot numbers 7.33 and 7.00 respectively. Shoot growth, rooting and survival after acclimatization were affected negatively at elevated levels of NH4Cl. The highest number of roots (19) was observed on a medium of 1.0 g/l NH4Cl followed by normal MS media (16). Survival after acclimatization was found to be 98% for plants derived from medium containing 4.5 and 3.8 g/l urea and KNO3 respectively followed by 95% for plants from medium containing 1.0 g/l NH4Cl. The experiment finally revealed that the three nitrogen salts at specific levels can be used as potential nitrogen source alternative instead of ammonium nitrate.
Abdominal compartment syndrome as a complication of insertion of a self-expandable metal stent in a patient with malignant bowel obstruction: a case report
Authors: Tanabe Taro , Tsukuda Genki , Hobo Takahiro , Yokoyama Noboru , Inoue Haruhiro 
Published: January 2023

Abstract
Background
A self-expandable metal stent is often placed as a bridge to elective surgical treatment of left-sided malignant obstruction of the colon because it allows for primary anastomosis without the need for a temporary stoma, which has a positive impact on the patient’s quality of life. However, although a relatively safe procedure, colonic stenting can have complications that require emergency surgery. This case report describes a rare case of abdominal compartment syndrome that occurred as a complication during colonic stenting.
Case presentation:
The patient was a 72-year-old woman who presented complaining of several days of constipation and loss of appetite. Computed tomography of the abdomen revealed obstruction of the sigmoid colon by a tumor. There were no symptoms or computed tomography findings to suggest perforation. Therefore, an attempt was made to insert a self-expandable metal stent. Acute respiratory disturbance and a change in consciousness occurred during the stenting procedure, with marked abdominal distention. Abdominal compartment syndrome was diagnosed and treated by decompressive laparotomy.
Conclusions
To the best of our knowledge, this is the first reported case of abdominal compartment syndrome as a complication of colonic stenting. The possibility of abdominal compartment syndrome should be considered if acute respiratory disturbance or altered consciousness occurs during placement of a colonic stent.
Opposite ends of the spectrum: Is emotional eating present more barriers to applying mindful eating and intuitive eating strategies for females?
Authors: KAHRIMAN Meryem , KAYA Neşe 
Published: January 2023

Abstract
Background: There are few studies in the literature examining the role of gender in the relationships between intuitive eating, mindful eating, dietary restraint, and emotional eating. It is important to reveal the role of gender in these relationships. Therefore, in this study, we aimed to evaluate the relationship between intuitive eating, mindful eating, emotional eating and restraint eating behaviors and the role of gender in these relationships.
Methods:This study was conducted with 522 participants aged 18-65. Questionnaire about sociodemographic characteristics and general eating habits, Intuitive Eating Scale-2 (IES-2), Mindful Eating Questionnaire-30 (MEQ-30), and Emotional Eating and Restraint Eating Subscale of Dutch Eating Behavior Questionnaire (DEBQ) were applied to the participants.
Results: DEBQ Restraint Eating, and Emotional Eating subscale scores were found to be higher in females. The total scores of IES-2 and MEQ-30 were found to be higher in the group with Body Mass Index (BMI)<25, and the DEBQ Emotional Eating subscale score was found to be higher in the group with BMI≥25. The negative correlation between DEBQ Emotional Eating subscale score and IES-2 total score was weak in males and strong in females. The negative relationship between MEQ-30 total score and DEBQ Emotional Eating subscale score was moderate in males and strong in females. It was determined that gender explained 27.7% of the relationship between the total score of IES-2 and the total score of MEQ-30, and 37.9% of the relationship between the total score of MEQ-30 and DEBQ Emotional Eating score.
Conclusions: Overweight and obese participants had lower levels of intuitive eating and mindful eating, while emotional eating levels were higher. In addition, it has been revealed that mindful eating and intuitive eating strategies are concepts that represent two opposite ends of a spectrum with emotional eating, and emotional eating can be an obstacle when these strategies are applied, especially in females.
In silico analysis of differentially expressed-aberrantly methylated genes in breast cancer for prognostic and therapeutic targets
Authors: Gadwal Ashita , Purohit Purvi , Khokhar Manoj , Vishnoi Jeewan Ram , Pareek Puneet , Choudhary Ramkaran , Elhence Poonam , Banerjee Mithu , Sharma Praveen 
Published: January 2023

Abstract
Introduction:
Breast cancer (BC) is the leading cause of death among women across the globe. Abnormal gene expression plays a crucial role in tumour progression, carcinogenesis and metastasis of BC. The alteration of gene expression may be through aberrant gene methylation. In the present study, differentially expressed genes which may be regulated by DNA methylation and their pathways associated with BC have been identified.
Methods:
Expression microarray datasets GSE10780, GSE10797, GSE21422, GSE42568, GSE61304, GSE61724 and one DNA methylation profile dataset GSE20713 were downloaded from Gene Expression Omnibus database (GEO). Differentially expressed-aberrantly methylated genes were identified using online Venn diagram tool. Based on fold change expression of differentially expressed-aberrantly methylated genes were chosen through heat map. Protein-protein interaction (PPI) network of the hub genes was constructed by Search Tool for the Retrieval of Interacting Genes (STRING). Gene expression and DNA methylation level of the hub genes were validated through UALCAN. Overall survival analysis of the hub genes was analysed through Kaplan Meier-plotter database for BC.
Results:
A total of 72 upregulated-hypomethylated genes and 92 downregulated-hypermethylated genes were obtained from GSE10780, GSE10797, GSE21422, GSE42568, GSE61304, GSE61724 and GSE20713 datasets by GEO2R and Venn diagram tool. PPI network of the upregulated-hypomethylated hub genes (MRGBP, MANF, ARF3, HIST1H3D, GSK3B, HJURP, GPSM2, MATN3, KDELR2, CEP55, GSPT1, COL11A1, and COL1A1) and downregulated-hypermethylated hub genes were constructed (APOD, DMD, RBPMS, NR3C2, HOXA9, AMKY2, KCTD9, and EDN1). All the differentially expressed hub genes expression was validated in UALCAN database. 4 in 13 upregulated-hypomethylated and 5 in 8 downregulated-hypermethylated hub genes to be significantly hypomethylated or hypermethylated in BC were confirmed using UALCAN database (p < 0.05). MANF, HIST1H3D, HJURP, GSK3B, GPSM2, MATN3, KDELR2, CEP55, COL1A1, APOD, RBPMS, NR3C2, HOXA9, ANKMY2, EDN1 were significantly (p < 0.05) associated with poor overall survival (OS).
Conclusion:
The identified aberrantly methylated-differentially expressed genes and their related pathways and function in BC can serve as novel diagnostic and prognostic biomarkers and therapeutic targets.
Analysis of salivary cortisol levels and listening effort in school-age children with unilateral hearing loss: investigating the impact of hearing aid use
Authors: de Matos Izabella Lima , Ferreira Maria Carolina , Dionisio Thiago José , De Araujo Cristiano Miranda , Santos Carlos Ferreira Dos , Mondelli Maria Fenanda Capoani Garcia 
Published: January 2023

Abstract
Arm: Listening effort is one of the consequences of Unilateral Hearing Loss (UHL). The aim of this clinical study was to assess the listening effort of school-aged children with UHL with salivary cortisol collection and a behavioral dual task assessment, with and without the use of hearing aid (HA) technology.
Results: There was no difference between speech recognition in silence and noise. Both conditions did not present statistically significant results with and without hearing aids. Greater statistically significant results (p= 0.013) were found before the HA fitting with the secondary set of dual-task. After the hearing aid fitting, a decreased response time was noted, and this result was statistically significant (p= 0.019) when compared to the response time before the HA fitting.
Conclusion: Results suggested that children fit with a HA in their affected ear performed better in the dual-task condition than without use of a hearing aid. Most participants showed a better response rate in the secondary task, mainly with regards to the number of correct answers and a better response time. Overall, a decrease of listening effort in the dual-task condition was found. Salivary cortisol measures of listening effort with our participants did not show any statistically significant findings after the use of a HA.
Fixed-Time Leader-Follower Consensus for Heterogeneous Multi-Agent Systems
Authors: Hua Xingxing , Dai Xin , Sun Shaoxin , Sun Yue 
Published: January 2023

Abstract
This article investigates the consensus problem for heterogeneous multi-agent systems (MASs) subject to nonlinear disturbance. A distributed fixed-time observer is considered for each follower. The proposed observer does not need any follower to become a neighbor of the leader. An improved distributed fixed-time observer is further proposed to avoid the situation in which the leader system information is unknown. This improved distributed fixed-time observer considers the output estimation errors of itself and its neighbors concurrently. Then, the consensus tracking protocols are designed to compensate for the error between leader and follower. Finally, the effectiveness of the proposed dynamic control strategy is validated by a numerical example and further application to wireless power transfer gird.
Protective Strategies of Nurses encountering Covid 19: A Qualitative Study
Authors: Mehboodi farkhondeh , Zamanzadeh vahid , Rahmani Azad , Dianat Iman , Shabanloi Reza 
Published: January 2023

Abstract
Background: Since nurses have a critical role in managing infectious diseases outbreaks, it is necessary to conduct more research to find the factors and risks leading to occupational injuries, as well as their individual action on protective measures in the event of epidemics. The aim of this study was to explain the protective strategies adopted by nurses during the Covid-19 pandemic.
Methods: This qualitative study was performed with the participation of 13 nurses caring for patients infected with Covid-19 in five medical centre in north-western Iran. Data were collected using non-structured qualitative and face-to-face interviews, and analysed by Graneheim and Lundman's content analysis method.
Results: Three main categories emerged: Normalization; Avoidance; and logical encounter.
Conclusion: Participants were using a wide range of strategies to protect themselves that each one leads to various individual and organizational consequences. By knowing these strategies, nursing managers can maintain the workforce and contribute to the productivity of the organization.
Systematic Patriarchy: A Social Determinant of Gender-Based Discrimination Interacting with Child Development and Mental Health
Authors: GUPTA MAYANK 
Published: January 2023

Abstract
The systematic oppression of women and gender-based discrimination has deep roots in human civilization. As evident in both written texts and widespread practices, conscious and unconscious biases associated with patriarchy have been—and continue to be—interlaced with power struggles, control, and conformity enforced by the male-dominant cultures of the time. Brought into bold relief in this pandemic, recent dramatic events (the tragic murder of George Floyd and the overturning of Roe v. Wade, for example) have heightened social outrage against bias, racism, and bigotry and have also brought us to an inflection point demanding our better understanding of the pernicious and long-term mental health effects of patriarchy. There are compelling grounds to further expand their construct, but efforts to do so in psychiatric phenomenology have, until now, failed to gain momentum and substantive attention. The resistance may in part lie in misconceptions that patriarchy is supported by archetypal endowments of the collective unconscious constitutive of shared societal beliefs. While many continue to live with the adverse experiences associated with patriarchy within the current times, critics have argued that our concepts about patriarchy are not empirical enough. Empirically supported deconstruction is indeed necessary to debunk misinformed notions that undermine women’s equality.
Direct structure-determination of nodavirus in solution by x-ray ensemble diffraction microscopy
Authors: Chen Ning-Jung , Yeh Chia-Hui , Cao Huai-Yu , Chen Nai-Chi , Chen Chun-Jung , Tsai Yi-Wei , Chen Chun-Yu , Lin Jhih-Min , Huang Yu-Shan , Chen Chien-Chun , Yang Jhih-Heng 
Published: January 2023

Abstract
The structures of biomacromolecules are conventionally characterized by crystallography and cryogenic electron microscopy . The requirements of sample preparation limit the understanding of the specimens in their native states. Small-angle x-ray scattering (SAXS) has the capability of obtaining structural information from biological specimens in solution. However, resolving the structure from the acquired one-dimensional (1D) diffraction data requires the prior knowledge of the sample, and no unique solution can be guaranteed. Coherent diffraction imaging (CDI) provides excellent uniqueness in 2D/3D phase retrieval while the resolution is restricted by the poor signal-to-noise ratio at high-angle scattering. Here we combine CDI and SAXS to directly image a 19-nm-sized nodavirus particle in solution and determine the core-shell density distribution at a 1.3 nm pixel resolution. With 77,170 diffraction patterns summarized from randomly distributed nodavirus particles, the structural information can be obtained from the diffraction intensity alone without preknowledge. The hollow density distribution of a nodavirus particle revealed by our reconstruction is consistent with the structural determinations from crystallography and cryogenic electron microscopy. We believe this work represents a new protocol for characterizing the structures of macromolecules in solution from accumulated x-ray scattering data.
A retrospective analysis of laparoendoscopic single-site myomectomy in giant uterine myoma
Authors: Yu Hongxia , Zhang Wendi , Zhang Shoufeng , Tang Huimin , Chen Mengyue , Zhao Mengru , Yang Yun , Tang Bin , Zheng Hong , Wei Weiwei , Shi Ruxia , Xia Bairong , Chen Jiming 
Published: January 2023

Abstract
Objectives
To investigate the safety and feasibility of laparoendoscopic single-site surgery (LESS) for the treatment of giant uterine myoma (diameter ≥ 8.0cm).
Methods
A retrospective analysis was made on 60 cases of uterine myoma (diameter ≥ 8.0 cm) who underwent laparoscopic myomectomy in Changzhou Second People's Hospital of Nanjing Medical University between April 2020 and April 2022. The patients were divided into a single-site laparoscopic group (LESS group) and a traditional multi-port laparoscopic surgery (MPLS group), with 20 patients in the study group (LESS group) and 40 patients in the control group (MPLS group). Observation and comparison were made between the two groups on demographic, perioperative, and follow-up data.
Results
All 60 surgeries were successfully completed without conversion. There was no statistically significant difference in estimated blood loss, and the first exhaust time after surgery between the two groups (P > 0.05); the operative time was longer in the LESS group than in the control group (P < 0.05); the length of hospital stay was shorter in the LESS group than in the control group (P < 0.05); The 24-hour visual analogue score (VAS) and body image score (BIS) were lower in the LESS group than in the control group (P < 0.05); the cosmetic score (CS) of the LESS was higher than that of the control group (P < 0.05).
Conclusions
Laparoendoscopic single-site myomectomy may be safe, feasible, and effective for the treatment of giant myoma (diameter ≥ 8.0cm). Compared with conventional multi-port laparoscopy, Laparoendoscopic single-site takes relatively longer to perform, but the hospital stay is relatively shorter, the postoperative pain is less, and the advantages of the aesthetic incision are outstanding, which meet the aesthetic needs of women and deserve a further clinical promotion.