bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of krill oil on Wnt/β-catenin signaling pathway in acetaminophen-induced acute liver injury in mice
Authors: Şahin Yaşar , Devrim Alparslan Kadir , Alçığır Mehmet Eray , Şenol Ali , Ekici Hüsamettin , Devrim Tuba , Sudağıdan Mert , Yıldırım Ebru , Çınar Miyase , Türkmen Merve Bişkin , Peker Seydi Ali 
Published: May 2023

Abstract

Objectives
This study investigated the effect of krill oil (KO) on liver damage caused by acetaminophen (APAP).

Methods
In the present study, the control and APAP groups were given distilled water by gavage for 14 days. In addition, the KO and APAP+KO groups were given 500 mg/kg krill oil by gavage for 14 days. At the end of 14 days, 0.9 % sodium chloride solution (saline solution) administration was applied intraperitoneally to the control and KO groups. Meanwhile, 220 mg/kg acetaminophen was administered to the APAP and APAP+KO groups. While some biochemical parameters in plasma were examined, some oxidative stress parameters in plasma and liver tissue were evaluated. Apoptotic and inflammatory responses of some primer sequences determined by quantitative Real-Time PCR (qPCR) in liver tissue. After histopathological examination of liver tissue, immunohistochemical analysis was performed with Wnt inhibitory factor-1 (Wif-1), beta-catenin (β-Catenin), and 8-hydroxy-2′-deoxyguanosine (8-OHdG).

Results
The Wif-1 positivity in hepatocytes increased significantly in the APAP group (5.29 ± 0.71) compared to the control (1.14 ± 0.51), and KO (2.14 ± 0.55) groups (p<0.001). The 8-OHdG positivity in hepatocytes increased significantly in the APAP group (19.57 ± 0.58) compared to the control (0.43 ± 0.20), KO (3.57 ± 0.48), and APAP+KO (4.00 ± 2.53) groups (p<0.001).

Conclusions
As a result, krill oil could be used as a nutritional supplement to protect the liver against acetaminophen-induced liver injury.
Virtual Reality to Assess Resident Recognition of Impending Respiratory Failure During COVID-19
Authors: Odum James D. , Real Francis J. , Rice Joshua , Meisman Andrea , Sahay Rashmi , Zhang Bin , Zackoff Matthew W. 
Published: May 2023

OBJECTIVES
To assess the performance of pediatric residents in recognizing a decompensating patient with impending respiratory failure and appropriately escalating care using a virtual reality (VR) simulated case of an infant with bronchiolitis after an extended period of decreased clinical volumes during the coronavirus disease 2019 (COVID-19) pandemic.

METHODS
Sixty-two pediatric residents at a single academic pediatric referral center engaged in a 30-minute VR simulation on respiratory failure in a 3-month-old admitted to the pediatric hospital medicine service with bronchiolitis. This occurred in a socially distant manner across the Zoom platform during the COVID-19 pandemic (January–April 2021). Residents were assessed on their ability to (1) recognize altered mental status (AMS), (2) designate clinical status as “(impending) respiratory failure,” and (3) escalate care. Statistical differences between and across postgraduate year (PGY) levels were examined using χ2 or Fisher’s exact test, followed by pairwise comparison and posthoc multiple testing using the Hochberg test.

RESULTS
Among all residents, 53% successfully recognized AMS, 16% identified respiratory failure, and 23% escalated care. No significant differences were seen across PGY levels for recognizing AMS or identifying respiratory failure. PGY3+ residents were more likely to escalate care than PGY2 residents (P = .05).

CONCLUSIONS
In the setting of an extended period with decreased clinical volumes during the COVID-19 pandemic, pediatric residents across all PGY levels demonstrated challenges with identifying (impending) respiratory failure and appropriately escalating care during VR simulations. Though limited, VR simulation may serve as a safe adjunct for clinical training and assessment during times of decreased clinical exposure.
Suture and glue-free autologous graft for pterygium surgery
Authors: Puttaswamy Shashikala , Shetty Shruthi Das 
Published: May 2023

Of various techniques of pteryium surgeries to prevent recurrence, conjunctival limbal auto graft has been the most successful adjuvant since the studies have shown the role of limbal stem cell deficiency (LSCD) as pathology of pterygium. The most common method of auto-graft fixation post pterygium excision is suturing; with drawbacks of prolonged operating time and postoperative discomfort. Replacing sutures with tissue adhesives may shorten the operating time, improve postoperative comfort; however, the major concern of the commercial fibrin glue is the cost and the potential risk of transmission of infection.To study, 1. The efficacy and complications of suture free and glue free conjunctival-limbal auto-graft for the management of pterygia, 2. The outcome of surgeries by two different qualified surgeons Prospective, interventional case study of 108 consecutive cases of pterygia that had excision with conjunctival-limbal auto-grafting without using glue or sutures. The operated eye patched for a day and followed up on day 1, 3 and 7, 6 weeks and 6 months; watched for hemorrhage beneath the graft, graft retraction, chemosis, recurrence or any other complication. The mean age of the patients was 40.7 years (range, 25 – 68 years) with female preponderance (60.2%) and nasal pterygia in 65.7 %. Not a single graft was dislodged and none had recurrence in the follow up period of 9-20 months. Graft retraction occurred in 7 cases (6.5%) and one patient had sub-graft hemorrhage which lasted for nine days. 76.9% had no discomfort even in immediate post-operative period. The results were comparable among patients of both the surgeons.Suture- and glue-freelimbal conjunctival auto-grafting following pterygium excision is a safe, effective and economical option for the management of both primary and recurrent pterygium. Learning curve is short and consistently similar results possible in different hands.
IMPLEMENTAÇÃO DAS SALAS ESTUDIOTECA E BIBLIOTECA E O PROGRAMA JUSTIÇA RESTAURATIVA NA REDE MUNICIPAL DE ENSINO DE SANTOS, SP
Authors: Alonso Verginia Soares , Barrocas Renata 
Published: May 2023

O presente artigo tem como propósito apresentar o processo que envolveu a implementação das salas Estudioteca e Biblioteca que atendem o Ensino Fundamental II na rede de ensino de Santos, cidade localizada no litoral do estado de São Paulo. A metodologia utilizada foi a aplicação de questionário, com questões abertas e fechadas, a três grupos de sujeitos. O primeiro grupo, composto por gestores da Seduc, o segundo grupo, por coordenadores pedagógicos, e o terceiro grupo composto por catorze professores resultando em uma análise de dados quantitativos e qualitativos. A implementação das salas Estudioteca e Biblioteca, e dos recursos tecnológicos nelas existentes, aconteceu por intermédio do Programa Justiça Restaurativa em parceria com o Instituto Tellus e a empresa privada Valor da Logística Integrada. Conclui-se que o fazer pedagógico de coordenadores e professores das unidades contempladas se fará produtivo em virtude de formações continuadas e propostas didáticas que invistam no uso do equipamento e inovem as metodologias das aulas.
La discriminación racial, étnica y social en el Perú: balance crítico de la evidencia empírica reciente
Authors: Santos Martín 
Published: May 2023

La discriminación, en tanto fenómeno social, constituye un tema de creciente interés en las ciencias sociales peruanas. En este contexto, el presente artículo tiene como objetivo principal realizar una evaluación crítica de esta literatura, poniendo énfasis en la discriminación racial, étnica y social. En la primera parte de este trabajo se presenta la literatura relevante en los siguientes ámbitos: empleo e ingresos, empresa, salud, educación, relación con entidades públicas y vida cotidiana. En la segunda parte se realiza una evaluación crítica, teniendo en cuenta los siguientes ejes: las preguntas de investigación, las perspectivas teóricas y las categorías conceptuales, la forma de medir el concepto de «raza», las preguntas que se plantean para medir el concepto de discriminación, las formas de identificar la existencia de discriminación y las interpretaciones (discutibles) que se hacen de los resultados obtenidos. El artículo concluye sugiriendo vacíos en la literatura, los cuales deben ser atendidos.
Mamá, ¿ya estás viniendo? Varones y mujeres proveedores de recursos y cuidados
Authors: Valdivia Santa Cruz Segundo 
Published: May 2023

Hemos investigado cómo los cambios por los que han pasado en los últimos tiempos el trabajo, la familia y la relación trabajo-familia han afectado a su vez a la relación y la división entre el trabajo remunerado y el trabajo familiar o doméstico de cuidados, sobre todo en familias donde tanto el varón como la mujer tienen trabajos remunerados. Nuestros principales hallazgos y reflexiones están relacio- nados con: (i) los roles del varón y la mujer: en la proveeduría de recursos, la maternidad y paternidad;(ii) la organización del tiempo en las familias y las condiciones de flexibilidad en los trabajos; (iii) las tensiones en la relación trabajo-familia; (iv) las prácticas en las tareas domésticas y de cuidados, y (v) las confrontaciones de poder en la interacción cotidiana para la realización de la división del trabajo.
Los circuitos políticos: cambios institucionales y nuevos movilizadores de la organización política estudiantil en el Perú
Authors: Chávez Angeles Noelia 
Published: May 2023

El Perú no cuenta con un movimiento estudiantil articulado y organizado que busque mejorar la calidad de las universidades públicas y privadas. No obstante, los estudiantes continúan agrupándose al interior de los claustros para postular a cargos de representación en el gobierno universitario. Este es el caso de la Universidad Nacional de la Amazonía Peruana (UNAP), donde aumentaron los grupos estudiantiles en los últimos años sin una agenda clara que busque el desarrollo de la universidad. El presente artículo explora esta paradoja y busca comprender los nuevos movilizadores de la organización estudiantil en la UNAP. Encuentra, en primer lugar, que la política universitaria funciona bajo una lógica clientelar entre estudiantes y docentes dentro de los órganos de gobierno universitario, pero además que hay un re-interés estudiantil por la política local y regional a raíz del proceso de descentralización.Las organizaciones estudiantiles estarían funcionando también como operadores políticos y sustitutos partidarios de los movimientos regionales. La política grande continúa interviniendo en la política chica —y viceversa—, convirtiendo a la universidad y los estudiantes en actores relevantes para comprender la política subnacional y el funcionamiento de las «democracias sin partidos».
Persistir en el intento: cambios y permanencias en la identidad y rol de las ONG fundacionales en el Perú 1990-2016
Authors: Bobadilla Díaz Percy 
Published: May 2023

Esta investigación pretende mostrar los cambios organizacionales y las formas de intervención social que han venido asumiendo las ONG fundadas en el Perú entre las décadas de 1960 y 1980. A partir de 1990, estas ONG han sufrido una serie de cambios, principalmente en los discursos de desarrollo que orientan sus proyectos, en el tipo de relaciones que establecen con actores claves para ejecutarlos y en la manera de financiar su funcionamiento. Se observará que en el ámbito del desarrollo internacional y local se modifican las reglas del juego para las ONG: el Estado central y el sector empresarial incrementan su presencia en la promoción de proyectos de desarrollo; hay una mayor orientación al mercado y a la venta de servicios basados en una gestión eficiente; en un contexto de reducción de fondos provenientes de agencias de cooperación internacional. Esto generará el surgimiento de roles estratégicos como una manera de responder a estos cambios en el entorno institucional. Se notará que, si bien las estrategias se han modificado y utilizan nuevos enfoques —ligados al desarrollo humano, el género, medio ambiente, gobernabilidad, entre otros—, el sentido y significado de la acción social fundacional de las ONG se mantienen vigentes: el trabajo con poblaciones vulnerables y excluidas.
ANALISIS BIBLIOMETRIK: TREN PENELITIAN BERPIKIR KREATIF
Authors: Huda Nurul , Misbah Misbah , Miriam Sarah , Harto Muhdi , Rusmansyah Rusmansyah , Hamid Abdul , Muhammad Nurlaela 
Published: May 2023

Penelitian terkait Creative Thinking sudah banyak dilakukan beberapa tahun belakangan ini, maka agar mendapatkan pandangan yang menyeluruh terkait Creative Thinking Skills dan memahami trend penekanan penelitian pada masa sekarang. Penelitian ini digunakan untuk mengevaluasi tema yang paling relevan terkait dengan Creative Thinking Skills berbasis analisis bibliometrik, dengan input menggunakan kata kunci “Creative Thinking Skills” OR “Creative Thinking”. Data yang digunakan berbasis database Scopus karena perangkat lunak VOSviewer versi 1.6.18 yang diadopsi sebagai alat analisis bibliometrik untuk memvisualisasikan jaringan berupa penulis, negara, jurnal, dan kata kunci. Analisis yang dilakukan pada 1 Agustus 2022 menemukan total 96 dokumen dalam periode dari tahun 2013 hingga 2022. Hasil penelitian menunjukkan bahwa dalam sepuluh tahun terakhir jumlah publikasi tentang Creative Thinking mengalami naik turun secara periodik, tetapi mengalami publikasi tertinggi pada tahun 2017 sebanyak 17 dokumen. Indonesia adalah negara terproduktif di bidang publikasi Creative Thinking, menyumbang jumlah publikasi terbesar di dunia. Analisis kata kunci menunjukkan bahwa studi tentang Creative Thinking dalam dua dekade terakhir berfokus pada tema yang terkait 21st century skills, higher order thinking skills, thinking skills, pollution, sampling technique, experimental research, research instrumen, gender, dan literature reviews. Analisis bibliometrik yang disajikan memberikan informasi yang relevan tentang tema utama yang dipelajari terkait Creative Thinking.
INSPECTR assay protocol for sensitive SARS-CoV-2 enabled by rolling circle amplification
Authors: Phillips Elizabeth A. , Silverman Adam D. , Joneja Aric , Liu Michael , Brown Carl , Carlson Paul , Coticchia Christine , Shytle Kristen , Larsen Alex , Goyal Nadish , Cai Vincent , Huang Jason , Hickey Jennifer E. , Ryan Emily , Acheampong Joycelynn , Ramesh Pradeep , Collins James J. , Blake William J. 
Published: May 2023

Abstract
This protocol defines the process for executing an INSPECTR assay to sensitively detect SARS-CoV-2 gRNA in a fully ambient temperature workflow. INSPECTR (for internal splint-pairing expression-cassette translation reaction), leverages the target-specific splinted ligation of DNA probes to generate expression cassettes that can be flexibly designed for the cell-free synthesis of reporter proteins. One implementation of INSPECTR incorporates rolling circle amplification, which is described in this protocol. This protocol entails five ambient temperature steps including target-splinted probe ligation, exonuclease digestion, hyperbranched rolling circle amplification, in vitro transcription and translation, and lateral flow detection. This implementation of the INSPECTR assay demonstrates the utility of synthetic biology and cell-free systems wherever low-cost and decentralized nucleic acid sensing is of value.
PASSAGING HUMAN INTESTINAL ORGANOIDS AND MONOLAYERS SET UP
Authors: Carmona Noelia , Randazzo Walter 
Published: May 2023

Abstract
Human intestinal enteroids (HIE) have been found to support enteric virus replication, which is especially relevant for studying human rotavirus and human norovirus infections. Thus, the HIE system is being used as a biologically significant human system to assess the efficiency of preventive and control strategies to prevent infection, which is relevant in clinical, therapeutics, and food safety fields. This protocol is intended to be used for the maintenance of human intestinal enteroids (HIE) (Procedure A) and set up of single cell monolayers to be differentiated and infected for viral replication (Procedure B). The procedures described are based on the original publications on human rotavirus and human norovirus replication in HIE (Ettayebi et al., 2016; Saxena et al., 2015; Zou et al., 2019), with the modifications implemented elsewhere (Ettayebi et al., 2021) and at VISAFElab for making the routinely handling of the system more practical. The main novel implementation refers to the use of the gentle cell dissociation reagent (produced by Stem Cell Technologies) to dissociate HIE domes for routine passaging or for producing single-cell suspensions for monolayers set up. Those modifications have been proven to support the human norovirus and human rotavirus replication features originally described for the HIE system.
Breast cancer characterization in cadavers
Authors: Boligo Sofia , Cardoso Vasco , Sidiropoulou Zacharoula , Cardoso Fátima 
Published: May 2023

Abstract
This protocol describes a technique for data collection in autopsies corpses, and data analysis of breast cancer epidemiological patterns.
The benefit of forensic autopsies lies in the relatively uniform age distribution of the population under study, unlike hospital samples. Only five publications exist in the international literature based on medico-legal autopsies that were designed to define the ‘natural reservoir’ of the disease.
According to the standard procedure, once the eligibility criteria have been fulfilled and the sample collection authorisation cleared, bilateral subcutaneous modified radical mastectomy in each fresh cadaver is performed. Tissues are subsequently transported within an appropriate container, and submitted for inspection, palpation, ultrasound and mammography by breast radiologists. The imaging of the collected tissues is performed using the GE Healthcare digital mammography system, Senographe EssentialTM, using an X-ray beam of 27 kV and 10-15 decanewtons (daN) compression, depending on tissue density and size. Breast tissue, classified as Breast Imaging Reporting and Data System (BI-RADS) category 3 or higher, is submitted to wire-guided surgical biopsy by a breast surgeon. These samples are subsequently analysed in the pathology department.
Intravenous Cyclophosphamide Therapy for Patients with Severe Ocular Inflammatory Diseases Who Failed Other Immunomodulatory Therapies
Authors: Karaca Irmak , Tran Elaine , Park Sung Who , Bromeo Albert John , Khojasteh Hassan , Tran Anh , Yavari Negin , Akhavanrezayat Amir , Yasar Cigdem , Uludag Gunay , Hassan Muhammad , Or Chris , Ghoraba Hashem , Do Diana , Dong Quan 
Published: May 2023

Abstract
Purpose To assess the safety and efficacy of cyclophosphamide (CP) therapy for patients with severe ocular inflammatory diseases who failed other immunomodulatory therapies (IMTs).Methods Medical records of 1295 patients who presented to the Uveitis Clinic at the Byers Eye Institute at Stanford between 2017 and 2022 were reviewed. Seven patients (10 eyes) who received CP therapy for ocular inflammatory diseases with at least one year of follow-up were included.Results The mean age of the patients (4 males, 3 females) was 61.6 ± 14.9 (43.0–89.0) years. Clinical diagnoses included scleromalacia perforans (4 eyes), anterior necrotizing scleritis (1 eye), peripheral ulcerative keratitis (2 eyes), orbital pseudotumor (1 eye), HLA-B27 associated panuveitis and retinal vasculitis (2 eyes). Ocular disease was idiopathic in 3 patients, and was associated with rheumatoid arthritis, IgG-4 sclerosing disease, dermatomyositis, and ankylosing spondylitis in 1 patient each. All the patients had history of previous IMT use including methotrexate (5), mycophenolate mofetil (3), azathioprine (1), tacrolimus (1), adalimumab (2), infliximab (4), and rituximab (1). The mean follow-up time was 34.4 ± 11.0 (13–45) months, and mean duration of CP therapy was 11.9 ± 8.8 (5–28) months. Remission was achieved in 5 patients (71.4%). Four patients (57.1%) experienced transient leukopenia (white blood cell count < 4000/mL).Conclusion CP therapy can be considered a potentially effective and relatively safe therapeutic option for patients with severe ocular inflammatory diseases who failed other IMTs including biologics (TNFa and CD20 inhibitors).
Can urban-level strategies foster nascent hydrogen industries?
Authors: Peng Yuan , White Lee , Cheng Wenting , Bai Xuemei 
Published: May 2023

Abstract
Many cities are developing strategies to foster rapid growth of the hydrogen industry for both environmental and economic benefits. Yet, the effectiveness of these strategies remains unclear. This paper addresses this gap. We extract the motivators and enabling factors of 21 frontrunner cities in China in implementing local hydrogen strategies. To examine whether frontrunner cities’ strategies lead to increased hydrogen industry development, a two-way fixed effects regression analysis is performed to the panel data of 19 frontrunner cities and 64 matching control cities during 2012-2021. We find that cities implementing hydrogen strategies saw increasing the number of firms, and, more tentatively, the investment scale. Our findings suggest cities can enable nascent hydrogen industry development, and have the potential to support broader energy system transition.
Successful Treatment of Post-COVID-19 Severe ANCA-Associated Vasculitis: Case Report
Authors: Ural Zeynep , Yıldırım Saliha , Şahin Osman Tamer , Tomar Veysel Baran , Zelyurt Nail , Koç Handenur , Türkoğlu Melda , Aygencel Gülbin , Erten Yasemin 
Published: May 2023

Abstract
Background
The SARS-CoV-2 pandemic has become the most serious health problem of today globally. Kidney involvement in patients with coronavirus disease 2019 (COVID-19) is common and associated with high mortality. Although acute tubular necrosis due to hemodynamic instability is the most common cause, other complex and destructive processes related to cytokine storm and activation of innate and adaptive immunity have also been reported.
Case presentation:
Herein, we present successful treatment of proteinase-3 anti-neutrophil cytoplasmic antibodies (PR3-ANCA)-associated vasculitis case presenting with severe pulmonary-renal syndrome as a rare and fatal complication of COVID-19 infection.
Conclusions
AAV is a serious disease and prompt treatment is one of the most important factors in patient survival.