bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nasopharyngeal tuberculosis suspected of malignancy: A case report
Authors: Chung Da Hyun , Lee Gil Joon 
Published: January 2025

Rationale:
Nasopharyngeal tuberculosis (TB), a rare form of tuberculosis outside the lungs, can affect any organ or tissue in the body. It is difficult to diagnose because of nonspecific symptoms, often leading to delayed confirmation after the initial patient visit. Clinical manifestations such as cervical lymphadenopathy and irregular mucosal surfaces can be challenging to distinguish from nasopharyngeal cancer or malignant lymphoma.

Patient concerns:
In this case report, we present a patient initially suspected of having a malignant disease based on abnormal nasopharyngeal imaging findings.

Diagnoses:
Further examination revealed chronic granulomatous inflammation, and subsequent tuberculosis polymerase chain reaction (TB-PCR) confirmed the diagnosis of tuberculosis.

Interventions:
The patient is currently receiving anti-TB treatment with a 4-drug regimen, which has shown a good response with continuous reduction in lesion size.

Outcomes:
After anti-TB treatment, the lesion size gradually decreased and continued to decrease, showing a significant response.

Lessons:
Awareness and precise evaluation are key to avoiding misdiagnosis, particularly when confronted with diverse clinical presentations. Extrapulmonary tuberculosis, although relatively rare, presents unique diagnostic challenges. Nasopharyngeal tuberculosis, in particular, lacks a definitive diagnostic method, often necessitating a combination of clinical suspicion, imaging studies, microbiological tests, and histopathological examination for confirmation. The absence of specific symptoms and the variability in presentation further compound the diagnostic dilemma. Given the potential consequences of misdiagnosis, further exploration and discussion on this issue are warranted. Enhanced awareness among healthcare providers, coupled with advancements in diagnostic modalities, are essential in ensuring timely and accurate differentiation between nasopharyngeal malignancies and tuberculosis, thereby facilitating appropriate management and improving patient outcomes.
Audio-Based Care for Managing Mental Health and Substance Use Disorders in Adults
Authors: Patel Sheila V. , Saavedra Lissette M. , Rodriguez Borja Ivette , Philbrick Sarah , Schwimmer Manny , Ruwala Richa , Viswanathan Meera 
Published: January 2025

Background:
Telehealth services can increase access to care by reducing barriers. Telephone-administered care, in particular, requires few resources and may be preferred by communities in areas that are systemically underserved. Understanding the effectiveness of audio-based care is important to combat the current mental health crisis and inform discussions related to reimbursement privileges.

Objectives:
We compared the effectiveness of audio-based care to usual care for managing mental health and substance use disorders (MHSUD).

Design:
We used systematic review methods to synthesize available evidence.

Studies:
We searched for English-language articles reporting randomized controlled trials (RCTs) of adults diagnosed with MHSUD published since 2012.

Outcomes:
We abstracted data on clinical outcomes, patient-reported health and quality of life, health care access and utilization, care quality and experience, and patient safety.

Results:
We included 31 RCTs of participants diagnosed with depression, post-traumatic stress disorder (PTSD), other serious mental illness (SMI), anxiety, insomnia, or substance use disorder (SUD). Most of the evidence was for interventions targeting depression, PTSD, and SUD. The evidence demonstrates promise for: (1) replacing in-person care with audio care for depression, other SMI, and SUD (very low to moderate certainty of comparable effectiveness); and (2) adding audio care to monitor or treat depression, PTSD, anxiety, insomnia, and SUD (low to moderate certainty of evidence favoring audio care for clinical outcomes).

Conclusions:
MHSUD can be managed with audio care in certain situations. However, more evidence is needed across conditions, and specifically for anxiety and other conditions for which no research was identified.
Epidemiology of epiretinal membranes using spectral-domain optical coherence tomography in an Iranian elderly population
Authors: Hashemi Hassan , Nabovati Payam , Aghamirsalim Mohammadreza , Hajizadeh Fedra , Hashemi Alireza , Khabazkhoob Mehdi 
Published: January 2025

SIGNIFICANCE
Epidemiological information about the epiretinal membrane is important for better clinical management and understanding of the nature and burden of this disease. There are some gaps in our understanding of the epidemiology of epiretinal membranes, particularly in Africa and the Middle East.

PURPOSE
This study aimed to determine the prevalence and risk factors of epiretinal membrane using spectral-domain optical coherence tomography (OCT) in an Iranian elderly population.

METHODS
This report is a part of a population-based study conducted on the elderly population 60 years and older from Tehran, the capital of Iran, using multistage stratified random cluster sampling. Study participants underwent measurement of visual acuity, autorefraction, subjective refraction, and slit-lamp biomicroscopy. The epiretinal membranes were diagnosed by a retinal specialist from OCT images (Spectralis OCT).

RESULTS
A total of 1298 individuals were analyzed for this report. Of these, 58.9% were female, and the mean age of the participants was 67.4 ± 6.4 years. The prevalence rates of all epiretinal membranes, primary epiretinal membrane, and secondary epiretinal membrane were 6.0% (95% confidence interval [CI], 4.6 to 7.5%), 4.7% (95% CI, 3.0 to 6.3%), and 10.1% (95% CI, 6.6 to 13.6%), respectively. According to the multiple logistic regression model, the interaction between age and diabetes (odds ratio [OR], 1.09; 95% CI, 1.01 to 1.18, p=0.048) and the presence of posterior vitreous detachment (OR, 2.89; 95% CI, 1.45 to 5.78, p=0.003) were significantly linked to a higher prevalence of primary epiretinal membrane. Additionally, a significant inverse relationship was found between the prevalence of primary epiretinal membrane and the number of years of education (OR, 0.91; 95% CI, 0.85 to 0.97; p=0.002).

CONCLUSIONS
The prevalence of primary epiretinal membrane in Tehran, Iran, was found to be lower than that reported in the majority of earlier studies. Posterior vitreous detachment, interaction between age and diabetes, and lower education level were risk factors of primary epiretinal membrane in this study.
Persistent Mortality Risk From Device-related Healthcare-associated Infection in Kidney Transplant Recipients Despite Multifaceted Interventions Action Calls for a Zero-tolerance Policy
Authors: Peruzzo Maria Bethânia , Oliveira Calegari Luana , Demarchi Foresto Renato , Tedesco-Silva Helio , Medina Pestana José , Requião-Moura Lúcio 
Published: January 2025

Background.
Although multifaceted control intervention actions (bundles) are highly effective in reducing the risk of device-related healthcare-associated infections (d-HAIs), no studies have explored their impact on the outcomes of kidney transplant recipients (KTRs) or the extent of risk reduction achievable through the bundle implementation.

Methods.
Seven hundred ninety-eight prevalent KTRs admitted to the intensive care unit (ICU) requiring invasive devices were included: 449 patients from the bundle preimplementation period and 349 from the postimplementation period. The primary outcome was mortality within 90 d of ICU admission. Using Poisson regression models, the magnitude of risk reduction for d-HAIs after the bundle implementation and the impact of d-HAIs on the risk of death was estimated.

Results.
The 90-d survival rate was significantly lower in patients with d-HAIs (37.7% versus 71.7%; P < 0.001). The bundle implementation reduced the risk of d-HAIs by 58% (relative risk, 0.42; P = 0.005). Despite the significant reduction in d-HAIs after the bundle implementation, d-HAIs were associated with a 2.6-fold higher risk of death (hazard ratio [HR], 2.63; P < 0.001) regardless of the study period. Additional variables associated with increased risk of death included age (HR, 1.03; P < 0.001), baseline immunosuppression (HR based on mycophenolate versus others 0.74; P = 0.02), time since transplantation (HR, 1.003; P < 0.001), platelet count at ICU admission (HR, 0.998; P < 0.001), and sepsis as the reason for ICU admission (HR, 1.67; P < 0.001).

Conclusions.
The persistent risk associated with d-HAIs, despite the implementation of multifaceted control intervention actions in an ICU specialized in KTR care, underscores the need for a zero-tolerance policy toward d-HAIs.
Spatiotemporal changes in soil properties predict plant health at continental scale
Authors: Wang Ningqi , Jiang Gaofei , Hou Yugang , Li Changqin , Meng Yuanduo , Chen Peijie , Zhang Yuling , Yang Tianjie , Wang Xiaofang , Mei Xinlan , Pommier Thomas , Banerjee Samiran , Rillig Matthias C. , Dini-Andreote Francisco , Friman Ville-Petri , Xu Yangchun , Jousset Alexandre , Shen Qirong , Wei Zhong 
Published: January 2025

AbstractAbiotic and biotic soil properties are strong predictors of plant yield globally1–5, but they become unreliable over large areas when plant health is threatened by pathogen6–8. Here we present a novel approach to predict plant health based on spatiotemporal changes in soil chemical and biological properties. We first demonstrate that plant health and soil properties consistently respond to environmental change (organic fertilization) regardless of the soil type or geographical origin. Second, we experimentally show that trackable shifts in soil properties reliably explain soil suppressiveness to theRalstonia solanacearumbacterial pathogen and that a scalable spatiotemporal model predicts plant health with 84% accuracy across multiple climatic zones and cropping systems. Our results suggest that this tight coupling between soil properties and plant health could facilitate the development of agricultural practices aimed at sustainably improving crop yields while safeguarding crop health.
An integrated single-cell and spatial transcriptomic atlas of thyroid cancer progression identifies prognostic fibroblast subpopulations
Authors: Loberg Matthew A. , Xu George J. , Chen Sheau-Chiann , Chen Hua-Chang , Wahoski Claudia C. , Caroland Kailey P. , Tigue Megan L. , Hartmann Heather A. , Gallant Jean-Nicolas , Phifer Courtney J. , Ocampo Andres , Wang Dayle K. , Fankhauser Reilly G. , Karunakaran Kirti A. , Wu Chia-Chin , Tarabichi Maxime , Shaddy Sophia M. , Netterville James L. , Rohde Sarah L. , Solorzano Carmen C. , Bischoff Lindsay A. , Baregamian Naira , Murphy Barbara A. , Choe Jennifer H. , Wang Jennifer R. , Huang Eric C. , Sheng Quanhu , Kagohara Luciane T. , Jaffee Elizabeth M. , Belcher Ryan H. , Lau Ken S. , Ye Fei , Lee Ethan , Weiss Vivian L. 
Published: January 2025

AbstractThyroid cancer progression from curable well-differentiated thyroid carcinoma to highly lethal anaplastic thyroid carcinoma is distinguished by tumor cell de-differentiation and recruitment of a robust stromal infiltrate. Combining an integrated thyroid cancer single-cell sequencing atlas with spatial transcriptomics and bulk RNA-sequencing, we define stromal cell subpopulations and tumor-stromal cross-talk occurring across the histologic and mutational spectrum of thyroid cancer. We identify distinct inflammatory and myofibroblastic cancer-associated fibroblast (iCAF and myCAF) populations and perivascular-like populations. The myCAF population is only found in malignant samples and is associated with tumor cell invasion,BRAFV600Emutation, lymph node metastasis, and disease progression. Tumor-adjacent myCAFs abut invasive tumor cells with a partial epithelial-to-mesenchymal phenotype. Tumor-distant iCAFs infiltrate inflammatory autoimmune thyroid lesions and anaplastic tumors. In summary, our study provides an integrated atlas of thyroid cancer fibroblast subtypes and spatial characterization at sites of tumor invasion and de-differentiation, defining the stromal reorganization central to disease progression.
Knockdown of PR-DUB subunitcalypsoin the developingDrosophilaeye and wing results in mis-patterned tissues with altered size and shape
Authors: Luf Max , Begani Priya , Bowcock Anne M. , Pfleger Cathie M. 
Published: January 2025

ABSTRACTThe deubiquitinating enzyme BAP1, the catalytic subunit of the PR-DUB complex, is implicated in several cancers, in the familial cancer syndrome BAP1 Tumor Predisposition Syndrome, and in the neurodevelopmental disorder Küry -Isidor syndrome. InDrosophila,there are numerous reports in the literature describing developmental patterning phenotypes for several chromatin regulators including the discovery of Polycomb itself, but corresponding adult morphological phenotypes caused by developmental dysregulation ofDrosophila BAP1orthologcalypso(caly) are less well-described. We report here that knockdown ofcalyin the eye and wing produce concomitant chromatin dysregulation phenotypes. RNAi tocalyin the early eye reduces survival and leads to changes in eye size and shape including eye outgrowths, some of which resemble homeotic transformations whereas others resemble tumor-like outgrowths seen in other fly cancer models. Mosaic eyes containingcalyloss-of-function tissue phenocopycalyRNAi. Knocking downcalyacross the wing disrupts wing shape and patterning including effects on wing vein pattern. This phenotypic characterization reinforces the growing body of literature detailing developmental mis-patterning driven by chromatin dysregulation and serves as a baseline for future mechanistic studies to understand the role of BAP1 in development and disease.ARTICLE SUMMARYPR-DUB catalytic subunit deubiquitinating enzyme BAP1 plays an important role in tumor suppression and chromatin regulation. Whereas many chromatin regulators are well-characterized for their roles in patterning, the mis-patterning phenotypes in adult structure for dysregulating BAP1 orthologcalypso(caly) in development are less well described. We report mis-patterned adult eye and wing phenotypes caused bycalyRNAi in the developing eye and wing respectively.
Modulation of cellular ageing regulates Integrated Stress Response signalling to control blood cell homeostasis
Authors: Ghosh Kishalay , Iyer Rohit Krishnan , Sood Saloni , Islam Mohamed Sabeelil , Labad Jyotsana G. , Khadilkar Rohan Jayant 
Published: January 2025

AbstractOrganismal ageing is associated with a decline in cellular and molecular functions. One of the hallmarks of ageing is stem cell exhaustion which occurs due to dynamic changes in the stem cell – niche microenvironment. Ageing affects stem cell potency impacting self-renewal and differentiation trajectories. Blood cell homeostasis in the hematopoietic system is tightly regulated by a fine balance between stemness and differentiation. Here, we employ theDrosophilahematopoietic organ, the larval lymph gland (LG) to investigate the impact of modulating cellular ageing on organ homeostasis. LG consists of a Posterior Signalling Center (PSC) that acts as a stem cell niche and maintains the prohemocytes housed in the Medullary Zone (MZ). Cortical Zone (CZ) consists of differentiated hemocytes namely plasmatocytes, crystal cells and lamellocytes. Intricate signalling networks active in the PSC and MZ orchestrate and regulate homeostasis. In this study, we characterize the effect of genetic perturbation of the molecular circuitry of ageing locally and systemically to investigate its effect on LG blood cell homeostasis. Genetic modulation of cellular ageing displays traditional hallmarks of ageing validating our model in the LG. Our results indicate that induction of accelerated ageing both locally and systemically leads to a reduction in stem cell niche size, DNA damage accumulation and increased progenitor differentiation whereas decelerated ageing shows an opposite trend. We show that the Integrated Stress Response (ISR) pathway is activated upon inducing accelerated ageing in the LG hemocytes possibly to recoup back to homeostatic conditions. Furthermore, LG’s of ISR pathway mutants or upon perturbation of ISR pathway components in prohemocytes show increased blood cell differentiation indicating disruption of homeostasis. Genetic epistasis analysis shows that ectopic over-expression of ISR pathway genes in an accelerated ageing scenario over and above the existing levels can rescue the defects in blood cell homeostasis. Overall, our study explores how modulation of cellular aging locally or systemically can impact tissue homeostasis. Our research paves way to understand the cellular mechanisms underlying an aged versus young stem cell-niche microenvironment and how its abrogation may lead to onset of disease.
microRNA-210 enhances cell survival and paracrine potential for cardiac cell therapy while targeting mitophagy
Authors: Alonaizan Rita , Purnama Ujang , Malandraki-Miller Sophia , Gunadasa-Rohling Mala , Lewis Andrew , Smart Nicola , Carr Carolyn 
Published: January 2025

AbstractThe therapeutic potential of presumed cardiac progenitor cells (CPCs) in heart regeneration has garnered significant interest, yet clinical trials have revealed limited efficacy due to challenges in cell survival, retention, and expansion. Priming CPCs to survive the hostile hypoxic environment may be key to enhancing their regenerative capacity. We demonstrate that microRNA-210 (miR-210), known for its role in hypoxic adaptation, significantly improves CPC survival by inhibiting apoptosis through the downregulation ofCasp8ap2, reduction of caspase activity, and decreased DNA fragmentation. Contrary to the expected induction of Bnip3-dependent mitophagy by hypoxia, miR-210 did not upregulateBnip3, indicating a distinct anti-apoptotic mechanism. Instead, miR-210 reduced markers of mitophagy and increased mitochondrial biogenesis and oxidative metabolism, suggesting a role in metabolic reprogramming. Furthermore, miR-210 enhanced the secretion of paracrine growth factors from CPCs, which promotedin vitroendothelial cell proliferation and cardiomyocyte survival. These findings elucidate the multifaceted role of miR-210 in CPC biology and its potential to enhance cell-based therapies for myocardial repair by promoting cell survival, metabolic adaptation, and paracrine signalling.
Development and integration of urban natural sciences for sustainable development and education
Authors: Gladkov Evgeny A. , Gladkova Olga Victorovna 
Published: January 2025

PurposeThis study aims to discuss the development of different fields of urban natural science as separate academic disciplines.Design/methodology/approachNotwithstanding the priority role of cities in our lives, many urban natural and technical sciences are not separate disciplines. There are many studies on urban biology, urban biotechnology, urban geology, the fields of urban engineering and medical sciences, but these directions have not yet emerged as separate disciplines. Perhaps the separation of many urban natural sciences into separate academic disciplines is premature. However, in order to improve the interconnection between the different interdisciplinary fields related to urban sciences, it is more appropriate to consider the different urban natural sciences as separate academic disciplines.FindingsThe integration of different urban natural sciences for sustainable urban development and environmental protection is of greatest interest. It is challenging to mitigate the adverse effects of ecological problems on the environment without integrating insights from diverse fields of natural science. Perhaps the allocation of urban natural sciences as a separate field of science is debatable. However, the designation of urban natural sciences as a separate field in educational programmes is relevant. The separate disciplines of “urban botany”, “urban biotechnology”, “urban physical geography” and other urban natural sciences in university curricula will contribute to the development of urban sciences.Originality/valueAt this time, there is not a single article where the various urban natural environmental sciences have been considered together. The design of educational programmes in the fields of urban natural sciences is important for education.
Enhanced melting dynamics of phase change material (PCM) based energy storage system combining modified fin and nanoparticles under solar irradiation
Authors: Nandi Anjan , Biswas Nirmalendu 
Published: January 2025

Purpose
This study aims to investigate the thermal performance enhancements of phase change materials (PCMs) through the integration of extended fins and CuO nanoparticles under the impact of solar irradiation. The research focuses on improving the melting behavior and thermal efficiency of PCM-based energy storage systems to facilitate the design of more efficient energy storage solutions.

Design/methodology/approach
The analysis is conducted on a top-heated rectangular thermal system filled with pure PCM and nanoparticle-enhanced PCM (NePCM) mixed with 0.01% Wt. CuO nanoparticles, with varying fin configurations considering PCM volume and surface area of fins constraint. The shape of the fin is modified from single to multiple numbers, maintaining the same surface area. The analysis is carried out both experimentally and numerically for the without fin case, and the study is extended numerically (utilizing the finite volume method) considering different sizes and positions of the fins. The study evaluates the impact of nanoparticle inclusion, fin geometry variations and the thermal performance of three different types of PCM (lauric acid, RT-35HC and P-58). Numerical results are validated against the in-house experimental results.

Findings
The study successfully validates the numerical simulations with experimental data, enhancing the credibility of the findings for real-world applications. The addition of 0.01% Wt. CuO nanoparticles to PCM resulted in a 16.36% enhancement in energy storage, as observed experimentally, whereas the numerical simulation showed an 8.55% increase. The inclusion of CuO nanoparticles accelerated the melting process across all fin configurations, with a notable enhancement parameter of 16.51% for the single fin arrangement. The introduction of a single fin structure increased the energy storage rate, but further additions of fins led to diminishing returns, with a maximum energy storage rate of 35.19 J/min achieved with CuO-enhanced PCM in the presence of single fin. The study also highlights RT-35HC as the most effective PCM, offering the highest energy storage and fastest melting speed, making it ideal for rapid thermal response applications.

Research limitations/implications
Future research could explore different types and concentrations of nanoparticles as well as a broader range of fin geometries and materials to further enhance the performance of PCM-based energy storage systems. Long-term experimental validation under real-world conditions would also enhance the applicability and reliability of the findings.

Originality/value
This study provides valuable insights into optimizing thermal energy storage systems by combining nanoparticle enhancement and fin geometry optimization. The results offer practical guidance for improving the efficiency and effectiveness of PCM-based energy storage units in various applications.