bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children
Authors: Patil Sandip , Chen Xiaowen , Dong Shaowei , Mai Huirong , Lopes Bruno Silvester , Liu Sixi , Wen Feiqiu 
Published: May 2023

IntroductionThe emergence of multidrug-resistant Pseudomonas aeruginosa poses a global threat, but the distribution and resistance profiling are unclear, especially in young children. Infections due to P. aeruginosa are common, associated with high mortality, and increasingly β-lactam drug resistant.MethodsWe studied the molecular epidemiology and antibiotic resistance mechanisms in 294 clinicalisolates of P. aeruginosa from a pediatric hospital in China. Non-duplicate isolates were recovered from clinical cases and were identified using an API-20 kit followed by antimicrobial susceptibility testing using the VITEK®2 compact system (BioMerieux, France) and also by broth dilution method. In addition, a double-disc synergy test for the ESBL/E-test for MBL was performed. The presence of beta-lactamases, plasmid types, and sequence types was determined by PCR and sequencing.ResultsFifty-six percent (n = 164) of the isolates were resistant to piperacillin–tazobactam, followed by cefepime (40%; n = 117), ceftazidime (39%; n = 115), imipenem (36%; n = 106), meropenem (33%; n = 97), and ciprofloxacin (32%; n = 94). Forty-two percent (n = 126) of the isolates were positive for ESBL according to the double-disc synergy test. The blaCTX-M-15 cephalosporinase was observed in 32% (n = 40/126), while 26% (n = 33/126) werepositive for blaNDM-1 carbapenemase. Aminoglycoside resistance gene aac(3)IIIawas observed in 16% (n = 20/126), and glycylcyclines resistance gene tet(A) was observed in 12% (n = 15/126) of the isolates. A total of 23 sequence types were detected, including ST1963 (12%; n = 16), followed by ST381 (11%; n = 14), ST234 (10%; n = 13), ST145 (58%; n = 10), ST304 (57%; n = 9), ST663 (5%; n = 7), and a novel strain. In ESBL-producing P. aeruginosa, 12 different Incompatibility groups (Inc) were observed, the most common being IncFI, IncFIS, and IncA/C. The MOBP was the most common plasmid type, followed by MOBH, MOBF, and MOBQ.DiscussionOur data suggest that the spread of antibiotic resistance is likely due toclonal spread and dissemination of different clinical strains of P. aeruginosa harbouring different plasmids. This is a growing threat in hospitals particularly in young children which needs robust prevention strategies.
A comparative 18F-FDG and an anti-PD-L1 probe PET/CT imaging of implant-associated Staphylococcus aureus osteomyelitis
Authors: Ren Shu-Qi , Ma Yuan , Fu Li-Lan , Hu Kong-Zhen , Liang Hao-Ran , Yu Bin , Tang Gang-Hua 
Published: May 2023

BackgroundEarly and accurate diagnosis of infection-induced osteomyelitis, which often involves increased PD-L1 expression, is crucial for better treatment outcomes. Radiolabeled anti-PD-L1 nuclear imaging allows for sensitive and non-invasive whole-body assessments of PD-L1 expression. This study aimed to compare the efficacy of 18F-FDG and an 18F-labeled PD-L1-binding peptide probe (18F-PD-L1P) in PET imaging of implant-associated Staphylococcus aureus osteomyelitis (IAOM).MethodsIn this study, we synthesized an anti-PD-L1 probe and compared its efficacy with 18F-FDG and 18F-PD-L1P in PET imaging of implant-associated Staphylococcus aureus osteomyelitis (IAOM). The %ID/g ratios (i.e., radioactivity ratios between the infected and non-infected sides) of both probes were evaluated for sensitivity and accuracy in post-infected 7-day tibias and post-infected 21 days, and the intensity of 18F-PD-L1P uptake was compared with pathological changes measured by PD-L1 immunohistochemistry (IHC).ResultsCompared with 18F-FDG, 18F-PDL1P demonstrated higher %ID/g ratios for both post-infected 7-day tibias (P=0.001) and post-infected 21 days (P=0.028). The intensity of 18F-PD-L1P uptake reflected the pathological changes of osteomyelitic bones. In comparison to 18F-FDG, 18F-PDL1P provides earlier and more sensitive detection of osteomyelitis caused by S. aureus.ConclusionOur findings suggest that the 18F-PDL1P probe is a promising tool for the early and accurate detection of osteomyelitis caused by S. aureus.
Hierarchical clustering-based framework for a posteriori exploration of Pareto fronts: application on the bi-objective next release problem
Authors: Casanova Carlos , Schab Esteban , Prado Lucas , Rottoli Giovanni Daián 
Published: May 2023

IntroductionWhen solving multi-objective combinatorial optimization problems using a search algorithm without a priori information, the result is a Pareto front. Selecting a solution from it is a laborious task if the number of solutions to be analyzed is large. This task would benefit from a systematic approach that facilitates the analysis, comparison and selection of a solution or a group of solutions based on the preferences of the decision makers. In the last decade, the research and development of algorithms for solving multi-objective combinatorial optimization problems has been growing steadily. In contrast, efforts in the a posteriori exploration of non-dominated solutions are still scarce.MethodsThis paper proposes an abstract framework based on hierarchical clustering in order to facilitate decision makers to explore such a Pareto front in search of a solution or a group of solutions according to their preferences. An extension of that abstract framework aimed at addressing the bi-objective Next Release Problem is presented, together with a Dashboard that implements that extension. Based on this implementation, two studies are conducted. The first is a usability study performed with a small group of experts. The second is a performance analysis based on computation time consumed by the clustering algorithm.ResultsThe results of the initial empirical usability study are promising and indicate directions for future improvements. The experts were able to correctly use the dashboard and properly interpret the visualizations in a very short time. In the same direction, the results of the performance comparison highlight the advantage of the hierarchical clustering-based approach in terms of response time.DiscussionBased on these excellent results, the extension of the framework to new problems is planned, as well as the implementation of new validity tests with expert decision makers using real-world data.
A novel therapeutic approach for endometriosis using adipose-derived stem cell-derived conditioned medium- A new hope for endometriotic patients in improving fertility
Authors: Huang S. Joseph , Huang Chun-Yen , Huang Yu-Hao , Cheng Jai-Hong , Yu Ya-Chun , Lai Jui-Chi , Hung Yi-Pei , Chang Chi-Chang , Shiu Li-Yen 
Published: May 2023

IntroductionEndometriosis is defined as the growth of endometrial glands and stromal cells in a heterotopic location with immune dysregulation. It usually leads to chronic pelvic pain and subfertility. Although various treatments are available, the recurrence rate remains high. Adipose tissue is an abundant source of multipotent mesenchymal adipose-derived stem cells (ADSCs). ADSCs display effects on not only tissue regeneration, but also immune regulation. Thus, the current study aims to test the effects of ADSCs on the growth of endometriosis.MethodsADSCs isolated from lipoaspiration-generated adipose tissue and their conditioned medium (ADSC-CM) were subjected to quality validation, including karyotyping as well as growth promotion and sterility tests for microbial contamination under Good Tissue Practice and Good Manufacturing Practice regulations. An autologous endometriosis mouse model was established by suturing endometrial tissue to peritoneal wall followed by treating with DMEM/F12 medium, ADSC-CM, ADSCs or ADSC-CM+ADSCs for 28 days. The area of endometriotic cysts and the degree of pelvic adhesion were measured. ICAM-1, VEGF and caspase 3 expression was assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry. Moreover, the mice were allowed to mate and deliver. The pregnancy outcomes were recorded. The ADSC-CM was subjected to proteomics analysis with further data mining with Ingenuity Pathway Analysis (IPA).ResultsBoth ADSC-CM and ADSCs passed quality validation. ADSC-CM reduced the area of endometriotic cysts. The inhibition by ADSC-CM was obliterated by adding ADSCs. The presence of ADSCs with or without ADSC-CM increased the peritoneal adhesion. ADSC-CM inhibited ICAM-1 and VEGF mRNA and protein expression, whereas the addition of ADSCs not only did not inhibit by itself, but also blocked the inhibition by ADSC-CM. The resorption rate was reduced by ADSC-CM. The number of live birth/dam and the survival rate of pup at 1 week-old were both increased by ADSC-CM in mice with endometriosis. IPA demonstrated that PTX3 was potentially critical for the inhibition of endometriosis by ADSC-CM due to its anti-inflammatory and antiangiogenic properties as well as its importance in implantation.ConclusionADSC-CM inhibited endometriosis development and improved pregnancy outcomes in mice. Potential translation to clinical treatment for human endometriosis is expected.
Cyclocarya paliurus leaves extracts alleviate metabolic phenotypes in Chinese T2DM patients by modulating gut microbiota and metabolites: a clinical randomized controlled trial
Authors: Peng Xiaojuan , Chen Sisi , Zhong Lu , Li Yuting , Wu Chutian , Zhong Lixian , Chen Weiwei , Yang Jinying , Zeng Jiahua , Tang Shaohui 
Published: May 2023

ObjectiveWe aimed to investigate the effect of Cyclocarya paliurus leaves extracts (CP) on glucose and blood lipid metabolism and its relationship with intestinal flora in type 2 diabetes mellitus (T2DM) patients.MethodsIn this open-label, 84-day randomized controlled trial, a total of 38 T2DM patients were randomly assigned to the CP group or the Glipizide group (G group) in a 2:1 ratio. T2DM-associated metabolic phenotypes, gut microbiota and metabolites including short-chain fatty acids (SCFAs) and bile acids (BAs) were detected.ResultsAt the end of intervention, CP, like Glipizide, significantly improved HbA1c level and other glucose metabolism parameters (fasting plasma glucose (FBG), 2-hour post-meal blood glucose (2hPBG), the area under curve of oral glucose tolerance test glucose (OGTT glucose AUC)). Moreover, CP also resulted in the significant improvement in the levels of blood lipid and blood pressure. Notably, the improvement in blood lipid(triglycerides (TG) and high-density lipoprotein cholesterol (HDL-c)) and blood pressure (diastolic blood pressure (DBP)) was significantly greater in the CP group compared with the G group. Furthermore, the liver and kidney function parameters did not significantly change in both CP group and the G group over the 84-day period. Additionally, the enrichment of potentially beneficial bacteria (Faecalibacterium and Akkermansia), SCFAs and unconjugated BAs and the depletion of potential pathogenic bacteria (Prevotella_9) and conjugated BAs were observed in the CP group, while the abundances of the gut microbial were kept stable in the G group after intervention.ConclusionCP displays a more beneficial effect in the alleviation of T2DM-associated metabolic phenotypes than glipizide by regulating gut microbiota and metabolites in T2DM patients, with no significant effects on liver and kidney function.
Identification of cavitation state of centrifugal pump based on current signal
Authors: Liang Chen , Hao Yan , Tengzhou Xie , Zhiguo Li 
Published: May 2023

Centrifugal pump, which is widely used in water conservancy, electric power, petrochemical, ship, aerospace, and other technical fields, is the core equipment used to ensure all kinds of energy transfer. Cavitation not only affects the service life of the centrifugal pump but also seriously impacts the reliability of the process flow or device system. Due to the influence of the life, position and number of vibration sensors, the existing cavitation fault feature identification accuracy is not enough. The state analysis and characteristic recognition of the current signal under the cavitation state of the centrifugal pump are conducted in this paper based on soft sensing technology, and the signal component and judgment threshold representing the cavitation state are obtained. The following results are presented. Under different critical cavitation numbers, a small number of bubbles appeared near the suction surface of the inlet edge of the impeller, which verified the reliability of criterion for the critical cavitation number when the head coefficient decreased by 3%. The overall accuracy of binary classification cavitation recognition based on the current signal is 12.9% higher than that of three classification cavitation recognition. The recognition rate of VMD decomposition under different working conditions is higher than that of EMD, in design conditions, for example, overall accuracy improved by 7.3%, which also indicates that the obtained cavitation information of each component by VMD decomposition is richer than that obtained by EMD decomposition. Comparing different working conditions, a large flow rate easily leads to cavitation and high recognition current rate, compared with the flow of 0.75 Q and 1.25 Q, the overall accuracy is improved by 9.6%.
IL11 activates the placental inflammasome to drive preeclampsia
Authors: Menkhorst Ellen , Santos Leilani L. , Zhou Wei , Yang Guannan , Winship Amy L. , Rainczuk Katarzyna E. , Nguyen Philana , Zhang Jian-Guo , Moore Paddy , Williams Michelle , Lê Cao Kim-Anh , Mansell Ashley , Dimitriadis Evdokia 
Published: May 2023

IntroductionPreeclampsia is a life-threatening disorder of pregnancy unique to humans. Interleukin (IL)11 is elevated in serum from pregnancies that subsequently develop early-onset preeclampsia and pharmacological elevation of IL11 in pregnant mice causes the development of early-onset preeclampsia-like features (hypertension, proteinuria, and fetal growth restriction). However, the mechanism by which IL11 drives preeclampsia is unknown.MethodPregnant mice were administered PEGylated (PEG)IL11 or control (PEG) from embryonic day (E)10-16 and the effect on inflammasome activation, systolic blood pressure (during gestation and at 50/90 days post-natal), placental development, and fetal/post-natal pup growth measured. RNAseq analysis was performed on E13 placenta. Human 1st trimester placental villi were treated with IL11 and the effect on inflammasome activation and pyroptosis identified by immunohistochemistry and ELISA.ResultPEGIL11 activated the placental inflammasome causing inflammation, fibrosis, and acute and chronic hypertension in wild-type mice. Global and placental-specific loss of the inflammasome adaptor protein Asc and global loss of the Nlrp3 sensor protein prevented PEGIL11-induced fibrosis and hypertension in mice but did not prevent PEGIL11-induced fetal growth restriction or stillbirths. RNA-sequencing and histology identified that PEGIL11 inhibited trophoblast differentiation towards spongiotrophoblast and syncytiotrophoblast lineages in mice and extravillous trophoblast lineages in human placental villi. DiscussionInhibition of ASC/NLRP3 inflammasome activity could prevent IL11-induced inflammation and fibrosis in various disease states including preeclampsia.
Effect and mechanism of chlorogenic acid on cognitive dysfunction in mice by lipopolysaccharide-induced neuroinflammation
Authors: Xiong Siyuan , Su Xuyang , Kang Yingjie , Si Junqiang , Wang Lu , Li Xinzhi , Ma Ketao 
Published: May 2023

BackgroundNeuroinflammation is an important factor causing numerous neurodegenerative pathologies. Inflammation can lead to abnormal neuronal structure and function and even death, followed by cognitive dysfunction. There is growing evidence that chlorogenic acid has anti-inflammatory effects and immunomodulatory activity.PurposeThe aim of this study was to elucidate the potential targets and molecular mechanisms of chlorogenic acid in the treatment of neuroinflammation.MethodsWe used the lipopolysaccharide-induced neuroinflammation mouse model and the lipopolysaccharide-stimulated BV-2 cells in vitro model. Behavioral scores and experiments were used to assess cognitive dysfunction in mice. HE staining and immunohistochemistry were used to assess neuronal damage in the mouse brain. Immunofluorescence detected microglia polarization in mouse brain. Western blot and flow cytometry detected the polarization of BV-2 cells. The migration of BV-2 cells was detected by wound healing assay and transwell assay. Potential targets for chlorogenic acid to exert protective effects were predicted by network pharmacology. These targets were then validated using molecular docking and experiments.ResultsThe results of in vivo experiments showed that chlorogenic acid had an obvious ameliorating effect on neuroinflammation-induced cognitive dysfunction. We found that chlorogenic acid was able to inhibit BV-2 cells M1 polarization and promote BV-2 cells M2 polarization in vitro while also inhibiting the abnormal migration of BV-2 cells. Based on the network pharmacology results, we identified the TNF signaling pathway as a key signaling pathway in which chlorogenic acid exerts anti-neuroinflammatory effects. Among them, Akt1, TNF, MMP9, PTGS2, MAPK1, MAPK14, and RELA are the core targets for chlorogenic acid to function.ConclusionChlorogenic acid can inhibit microglial polarization toward the M1 phenotype and improve neuroinflammation-induced cognitive dysfunction in mice by modulating these key targets in the TNF signaling pathway.
Efficacy and safety of chimeric antigen receptor T cell therapy in relapsed/refractory diffuse large B-cell lymphoma with different HBV status: a retrospective study from a single center
Authors: Kong Danqing , Ping Nana , Gao Xin , Zou Rui , Wang Peng , Wu Depei , Jin Zhengming , Qu Changju 
Published: May 2023

BackgroundChimeric antigen receptor T cell (CAR-T) therapy is an effective salvage treatment in relapsed or refractory(r/r) diffuse large B-cell lymphoma (DLBCL), but the impact of hepatitis B virus (HBV) infection has not been studied.Methods and resultsHere, 51 patients with r/r DLBCL receiving CAR-T therapy were enrolled and analyzed at the First Affiliated Hospital of Soochow University. The overall response rate and the complete remission rate (CR) of CAR-T therapy were 74.5% and 39.2%, respectively. With a median follow-up of 21.1 months after CAR-T, the probabilities of overall survival (OS) and progression-free survival (PFS) at 36 months were 43.4% and 28.7%, respectively. These patients were divided into three cohorts including chronic HBV infection group (n=6), resolved HBV infection group (n=25) and non-HBV infection group (n=20). Bone marrow involvement was significantly higher in the HBV infection group(P&lt;0.001), other basic characteristics before CAR-T therapy were comparable. Subgroup analysis showed that HBV infection status did not affect the efficacy of CAR-T therapy in CR rate, OS or PFS, and there was no significant difference in CAR-T related toxicities between three cohorts. Only one cirrhosis patient with chronic HBV infection experienced HBV reactivation.ConclusionsCAR-T therapy was effective and can be used safely in r/r DLBCL with HBV infection under proper monitoring and antiviral prophylaxis.
Gut epithelial barrier dysfunction in lupus triggers a differential humoral response against gut commensals
Authors: Botía-Sánchez María , Galicia Georgina , Albaladejo-Marico Lorena , Toro-Domínguez Daniel , Morell Maria , Marcos-Fernández Raquel , Margolles Abelardo , Alarcón-Riquelme Marta E. 
Published: May 2023

IntroductionSystemic lupus erythematosus is an autoimmune disease with multisystemic involvement including intestinal inflammation. Lupus-associated intestinal inflammation may alter the mucosal barrier where millions of commensals have a dynamic and selective interaction with the host immune system. Here, we investigated the consequences of the intestinal inflammation in a TLR7-mediated lupus model.MethodsIgA humoral and cellular response in the gut was measured. The barrier function of the gut epithelial layer was characterised. Also, microbiota composition in the fecal matter was analysed as well as the systemic humoral response to differential commensals.ResultsThe lupus-associated intestinal inflammation modifies the IgA+ B cell response in the gut-associated lymphoid tissue in association with dysbiosis. Intestinal inflammation alters the tight junction protein distribution in the epithelial barrier, which correlated with increased permeability of the intestinal barrier and changes in the microbiota composition. This permeability resulted in a differential humoral response against intestinal commensals.DiscussionLupus development can cause alterations in microbiota composition, allowing specific species to colonize only the lupus gut. Eventually, these alterations and the changes in gut permeability induced by intestinal inflammation could lead to bacterial translocation.
The distribution and emission of CO2, CH4 and light hydrocarbons in an anticyclonic eddy of the Kuroshio extension
Authors: Li Xiao-Jun , Wang Jian , Qiao Hao , Zhu Rui-Chen , Zhang Hong-Hai , Chen Zhao-Hui , Montgomery Andrew , Zheng Shan , Zhuang Guang-Chao 
Published: May 2023

Mesoscale eddies are energetic and swirling circulations that frequently occur in the open ocean. The effects of mesoscale eddies on the biogeochemical cycling of climate-relevant gases remain poorly constrained. We investigated the distribution and air-sea fluxes of CO2, methane, and five non–methane hydrocarbons (NMHCs) in an anticyclone eddy of Kuroshio Extension during September 2019. Within eddy core, intense stratification hindered the replenishment of nutrients and favored the growth of small-size phytoplankton, such as Prochlorococcus. Seawater pCO2 decreased from 406.1 μatm at the eddy outside to 377.5 μatm at the eddy core, accompanied by a decrease in surface seawater temperature from 26.7 °C to 25.2 °C. The vertical distribution of methane (0.3-9.9 nmol L-1) was influenced by the eddy process, with a maximum at 80 m in the eddy core, which might be attributed to the degradation of phosphonates sustained by Prochlorococcus. The concentrations of five NMHCs (ethane, ethylene, propane, propylene, and isoprene) ranged from 17.2-126.2, 36.7-168.1, 7.5-29.2, 22.6-64.1, 54.5-172.1, 3.5-27.9 pmol L-1, respectively. Isoprene correlated well with Chl-a concentrations at the eddy core, while no significant correlation was observed at the eddy outside. Air-sea fluxes of CO2 and isoprene associated with the eddy core were higher than those of the eddy outside, while the maximum ventilation of methane and other NMHCs (ethane, ethylene, propane, and propylene) was found at the eddy outside. Collectively, physical processes such as eddies impact the production and distribution of light hydrocarbons in seawater and further influence their regional emissions to the atmosphere.