bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robotically-controlled three-dimensional micro-ultrasound for prostate biopsy guidance
Authors: Vassallo Reid , Aleef Tajwar Abrar , Zeng Qi , Wodlinger Brian , Black Peter , Salcudean Septimiu E. 
Published: December 2022

AbstractPurposeProstate imaging to guide biopsy remains unsatisfactory, with current solutions suffering from high complexity and poor accuracy and reliability. One novel entrant into this field is microultrasound (microUS), which uses a high frequency imaging probe to achieve very high spatial resolution, and achieves prostate cancer detection rates equivalent to multiparametric magnetic resonance imaging (mpMRI). However, the ExactVu transrectal microUS probe has a unique geometry that makes it challenging to acquire controlled, repeatable three-dimensional (3D) transrectal ultrasound (TRUS) volumes. We describe the design, fabrication, and validation of a 3D acquisition system that allows for the accurate use of the ExactVu microUS device for volumetric prostate imaging.MethodsThe design uses a motorized, computer-controlled brachytherapy stepper to rotate the ExactVu transducer about its axis. We carry geometric validation using a phantom with known dimensions and we compare performance with magnetic resonance imaging (MRI) using a commercial quality assurance anthropomorphic prostate phantom.ResultsOur geometric validation shows accuracy of 1 mm or less in all three directions, and images of an anthropomorphic phantom qualitatively match those acquired using MRI and show good agreement quantitatively.ConclusionWe describe the first system to acquire robotically- controlled 3D microUS images using the ExactVu microUS system. The reconstructed 3D microUS images are accurate, which will allow for applications of the ExactVu microUS system in prostate specimen andin vivoimaging.
Asymmetric HIV-1 envelope trimers bound to one and two CD4 molecules are intermediates during membrane binding
Authors: Li Wenwei , Nand Elizabeth , Qin Zhuan , Grunst Michael W. , Grover Jonathan R. , Bess Julian W. , Lifson Jeffrey D. , Zwick Michael B. , Tagare Hemant D. , Uchil Pradeep D. , Mothes Walther 
Published: December 2022

AbstractHuman immunodeficiency virus 1 (HIV-1) infection is initiated by binding of the viral envelope glycoprotein (Env) to the cell-surface receptor CD4. Although high resolution structures of Env complexed with soluble domains of CD4 have been determined, the binding process is less understood on native membranes. Here, we apply cryo-electron tomography (cryo-ET) to monitor Env-CD4 interactions at membrane-membrane interfaces formed between HIV-1 and CD4-presenting virus-like particles. Env-CD4 complexes organized into clusters and rings, bringing opposing membranes closer together. Additionally, Env-CD4 clustering was dependent on capsid maturation. Subtomogram averaging and classification revealed that Env bound one, two, and finally three CD4 molecules, upon which Env adopted a partially open state. Our data indicate that asymmetric HIV-1 Env trimers bound to one and two CD4 molecules are detectable intermediates during virus binding to host cell membranes, which likely has consequences for antibody-mediated immune responses and vaccine immunogen design.
An Interplay of Phytoplankton Donor Species and Transformation of Released Compounds over Time Defines Bacterial Communities Following Phytoplankton DOMp Pulses
Authors: Eigemann Falk , Rahav Eyal , Grossart Hans-Peter , Aharonovich Dikla , Voss Maren , Sher Daniel 
Published: December 2022

ABSTRACTPhytoplankton-bacteria interactions are stimulated by phytoplankton-released dissolved organic matter (DOMp). Two factors that shape the accompanying bacterial community are i) the “donor” phytoplankton species, defining the initial composition of released DOMp, and ii) the DOMp transformation over time. We added phytoplankton DOM from two globally abundant species - the diatomSkeletonema marinoiand the cyanobacteriumProchlorococcusMIT9312 - to natural bacterial communities in the Eastern Mediterranean and determined the bacterial responses over a time-course of 72 h in terms of cell numbers, bacterial production (BP), alkaline phosphatase activity (APA), and changes in active bacterial community compositions based on rRNA amplicon sequencing. Both DOMp types were demonstrated to serve the bacterial community as carbon and potentially phosphorus source. Diatom-derived DOM induced higher BP and lower APA compared to cyanobacterium DOM after 24 h, but not after 48 and 72 h of incubation, and also maintained higher Shannon diversities over the course of the experiment, indicating a better bacterial accessibility and broader disposability of diatom derived DOM. Bacterial communities significantly differed between DOMp types as well as different incubation times, pointing to a certain bacterial specificity for the DOMp donor as well as a successive utilization of phytoplankton DOM by different bacterial taxa. The highest differences in bacterial community composition with DOMp types occurred shortly after additions, suggesting a high specificity towards highly bioavailable DOMp compounds. We conclude that phytoplankton associated bacterial communities are strongly shaped by an interplay between phytoplankton donor and the transformation of its released DOMp over time.IMPORTANCEPhytoplankton-bacteria interactions maintain biogeochemical cycles of global importance. Phytoplankton photosynthetically fix carbon dioxide and subsequently release the synthesized compounds as dissolved organic matter (DOMp), which becomes processed and recycled by heterotrophic bacteria. Yet, the combined effect of the phytoplankton donor species and time-dependent transformation of DOMp compounds on the accessibility to the bacterial community has not been explored until now. The diatomSkeletonema marinoiand the cyanobacteriumProchlorococcusMIT9312 are globally important phytoplankton species, and our study revealed that DOMp of both species was selectively incorporated by the bacterial community. The donor species had the highest impact shortly after DOMp appropriation, and its effect diminished over time. Our results improve the understanding of biogeochemical cycles between phytoplankton and bacteria, and solve yet unresolved questions of phytoplankton-bacteria interactions.
The contribution of Mediterranean connectivity to morphological variability in Iron Age sheep of the Eastern Mediterranean
Authors: Harding Sierra A. , Hadjikoumis Angelos , Vermeersch Shyama , Shafir Roee , Marom Nimrod 
Published: December 2022

AbstractThe movement of livestock across the Mediterranean is well-documented in the Neolithic era, but its significance during subsequent periods has received less attention. Here we start addressing this lacuna by analyzing astragal bone morphology from four coastal and inland sites in Israel and Cyprus, seeking potential evidence for maritime connections between sheep populations in the Iron Age eastern Mediterranean. Employing an established geometric morphometric protocol, we investigated the hypothesis that intra-site morphological variability is higher in coastal sites, reflecting greater genetic diversity in the livestock populations. While our findings support this hypothesis, the conclusions are constrained by contextual and sample size limitations.
A semi-field system for quantifyingAnopheles gambiaeattraction to human scent
Authors: Giraldo Diego , Rankin-Turner Stephanie , Corver Abel , Tauxe Genevieve M. , Gao Anne L. , Jackson Dorian M. , Simubali Limonty , Book Christopher , Stevenson Jennifer C. , Thuma Philip E. , Gordus Andrew , Mburu Monicah M. , Simulundu Edgar , McMeniman Conor J. 
Published: December 2022

AbstractVariability in the chemical composition of human scent has the potential to modulate mosquito attraction to certain humans. We have engineered a large-scale, semi-field system in Zambia for quantifying mosquito olfactory preferences towards whole body odor sourced from different humans under naturalistic conditions. In a flight cage arena with infrared tracking, we document that the African malaria mosquitoAnopheles gambiaehierarchically prefers to land on heated targets mimicking human skin temperature when they are baited with carbon dioxide (CO2) over background air, human body odor over CO2, and the scent of one individual over another. In a six-choice assay configuration, we further identify humans at both ends of the attractiveness spectrum whose scent is differentially attractive toAn. gambiaerelative to other individuals. We demonstrate integrative use of this multi-choice olfactory assay with whole body volatilomics, establishing a powerful method for discovery of human odorants modulating heterogeneity in biting risk at enhanced throughput.
Discovery and characterization of cyclic peptides selective for theC-terminal bromodomains of BET family proteins
Authors: Franck Charlotte , Patel Karishma , Walport Louise J , Christie Mary , Norman Alexander , Passioura Toby , Suga Hiroaki , Payne Richard J , Mackay Joel P 
Published: December 2022

SUMMARYDNA encoded cyclic peptide libraries offer unique opportunities to discover high-potency, high-specificity ligands directed against a target protein. We set out to explore the potential for such libraries to provide ligands that can distinguish between bromodomains from the closely related paralogues of the Bromodomain and ExtraTerminal domain (BET) family of epigenetic regulators. Analysis of peptides isolated from a screen against theC-terminal bromodomain of family member BRD2, together with new peptides discovered in previous screens against the corresponding domain from BRD3 and BRD4, reveals peptides with nanomolar and subnanomolar affinities. X-ray crystal structures of several of these bromodomain-peptide complexes reveal diverse structures and binding modes, which nevertheless display several conserved binding features. A subset of the peptides demonstrates significant paralogue-level specificity, though structural analysis does not reveal clear physicochemical explanations for this specificity. Our data demonstrate the power of cyclic peptides to discriminate between highly similar proteins with high potency and hint that differences in conformational dynamics between BET-family bromodomains might modulate binding affinities amongst family members for particular ligands.
SUMOylation of the Cardiac Sodium Channel NaV1.5 Modifies Inward Current and Cardiac Excitability
Authors: Yoon Jin-Young , Greiner Alexander M. , Jacobs Julia S. , Kim Young-Rae , Kutschke William , Matasic Daniel S. , Vikram Ajit , Gaddam Ravinder R , Mehdi Haider , Irani Kaikobad , London Barry 
Published: December 2022

AbstractBackgroundDecreased peak sodium current (INa) and increased late sodium current (INa,L), through the cardiac sodium channel NaV1.5 encoded bySCN5A, cause arrhythmias. Many NaV1.5 post-translational modifications have been reported by us and others. A recent report concluded that acute hypoxia increases INa,Lby increasing a Small Ubiquitin-like MOdifier (SUMOylation) at K442-NaV1.5.ObjectiveTo determine whether and by what mechanisms SUMOylation alters INa, INa,Land cardiac electrophysiology.MethodsSUMOylation of NaV1.5 was detected by immunoprecipitation and immunoblotting. INawas measured by patch clamp with/without SUMO1 overexpression in HEK293 cells expressing wild type (WT) or K442R-NaV1.5 and in neonatal rat cardiac myocytes (NRCMs). SUMOylation effects were studiedin vivoby electrocardiograms and ambulatory telemetry using Scn5a heterozygous knockout (SCN5A+/-) mice and the de-SUMOylating protein SENP2 (AAV9-SENP2) or the SUMOylation inhibitor anacardic acid. NaV1.5 trafficking was detected by immunofluorescence.ResultsNaV1.5 was SUMOylated in HEK293 cells, NRCMs and human heart tissue. HyperSUMOylation at NaV1.5-K442 increased INain NRCMs and in HEK cells overexpressing WT but not K442R-Nav1.5. SUMOylation did not alter other channel properties including INa,L. AAV9-SENP2 or anacardic acid treatment of SCN5A+/-mice decreased INa, prolonged QRS duration, and produced heart block and ventricular arrhythmias. SUMO1 overexpression enhanced membrane localization of NaV1.5.ConclusionSUMOylation of K442-Nav1.5 increases peak INawithout changing INa,L, at least in part by altering membrane abundance. Our findings do not support SUMOylation as a mechanism for changes in INa,L. Nav1.5 SUMOylation may modify arrhythmic risk in disease states and represents a potential target for pharmacological manipulation.
Residential satisfaction and quality of urban life: examining diverse housing environments
Authors: Koçak Güngör Merve , Terzi Fatih 
Published: December 2022

PurposeAs an important indicator of the quality of life of individuals, residential environments are continuing to evolve, due to the rapidly changing production–consumption relations. However, in this evolving process, the effect of the differentiated residential environments on the individuals' residential satisfaction remains unclear. This paper aims to measure the effects of the varying residential environments on the overall quality of urban life (QoUL) in Kayseri, one of the most developed cities in Central Anatolia.Design/methodology/approachIt is based on empirical data on the quality of life in the different residential environments of Kayseri. The research method used stratified purposeful sampling, and the household survey data were analyzed using factor analysis, multiple regression and ANOVA statistical methods.FindingsThe most influential factors on the overall QoUL of individuals living in different Kayseri residential neighborhoods were satisfaction with neighborhood and city-level urban services, neighborhood relations and belonging factor groups. The critical finding obtained in this study is that residential satisfaction in low-rise and compact form housing areas in Kayseri is higher compared to residential satisfaction in high-rise neighborhoods. This result reveals that the high-rise building typology that is dominant in Turkey's big cities should be seriously questioned, and urban development policies should be re-evaluated.Research limitations/implicationsThe study was designed to produce baseline data so that future changes in residential conditions as perceived by the residents of Kayseri could be monitored to support decisions for residential areas.Originality/valueComparative case studies, particularly on low-rise versus high-rise environments, are scarce. As a result, this research contributes to the field of comparative studies on residential environments.
Acceptance of in vitro meat and the role of food technology neophobia, dietary patterns and information – Empirical evidence for Germany
Authors: Heidmeier Anna Katharina , Teuber Ramona 
Published: December 2022

PurposeThe present study addresses acceptance of in vitro meat (IVM) among a predominantly student sample in Germany. It is investigated to which extent food technology neophobia, the currently followed diet and information treatments impact acceptance of IVM measured via the construct willingness to buy (WTB).Design/methodology/approachA quantitative online-survey was conducted in August 2020 using a between-subject design with three different information treatments and one control group. Moreover, the Food Technology Neophobia (FTN) scale was employed, For the statistical analysis, the χ2 and Kruskal–Wallis test were used. Additionally, a binary logit model was specified and estimated in order to investigate the determinants of willingness to buy IVM accounting for the effects of gender, age, vegetarianism/veganism, FTN, prior knowledge, information treatments and potential interaction effects.FindingsParticipants following a vegan or vegetarian diet exhibit a lower likelihood of IVM acceptance in comparison to participants following an omnivore diet. However, a considerable share of vegan and vegetarian participants expressed a positive WTB. Moreover, an increasing FTN score (i.e. an increase in food technology neophobia) goes along with a reduced likelihood of acceptance, while all three information treatments increase acceptance in comparison to the control group. The largest effect on acceptance could be found for the environmental benefit treatment.Practical implicationsThe findings show that especially among a young and highly educated sample the stressing of environmental benefits of IVM has a substantial impact on acceptance. This might be taken up in information and marketing campaigns once the product becomes available on the European market.Originality/valueSo far the empirical evidence on German consumers' acceptance of IVM is scarce. The present study addressed this research gap by focusing on a young sample with a high percentage of vegetarians and vegans and analyzing the role of food technology neophobia and different information treatments in a between-subject design.
Strength of HR signals and intent to stay: mediating role of psychological contract fulfillment
Authors: Bandyopadhyay Chiradip , Srivastava Kailash B.L. 
Published: December 2022

PurposeThe study examined the relationship between human resource (HR) signals and intent to stay (ITS) in an attributional framework. It also investigated the mediating role of psychological contract fulfillment in the relationship between the strength of HR signal and ITS.Design/methodology/approachThe data were collected from 460 managers in the manufacturing sector from eastern India using a structured questionnaire. The study adopted a mediated structural equation model to test the model fit and hypotheses.FindingsThe study confirmed the relationship between the strength of the H R signal and the ITS, and the mediating role of psychological contract fulfillment between the strengths of HR signals and ITS. The study indicates a positive role of the psychological contract fulfillment in strengthening the effect of HR signals on ITS.Originality/valueThe study adds to the body of knowledge by confirming a signaling role of HR practices within the firm having the attributional ability. It also indicates the role of psychological contract fulfillment in employee retention.
Gender diversity and SDG disclosure: the mediating role of the sustainability committee
Authors: Zampone Giovanni , Nicolò Giuseppe , Sannino Giuseppe , De Iorio Serena 
Published: December 2022

PurposeThe study examines the association between board gender diversity and Sustainable Development Goal (SDG) disclosure from an international and longitudinal perspective. It also investigates the role of the Sustainability Committee (SC) as a possible factor that can mediate the relationship between board gender diversity and SDG disclosure.Design/methodology/approachThe authors focused on the annual Communication on Progress (CoP) prepared annually by a sample of 526 companies from 39 countries and ten industry sectors along the 2017–2020 period to evaluate the SDG disclosure. Baron and Kenny's (1986) three-step model is estimated to test the impact of the presence of an SC on the SDG disclosure level and the mediating effect exerted by the SC on the relationship between board gender diversity and SDG disclosure.FindingsFindings shed light on the usefulness of the CoP as an alternative reporting tool to communicate progress against SDGs achievement, especially regarding SDGs 13 and 8. This study evidences that board gender diversity positively influences SDG disclosure. The relationship between board gender diversity and SDG disclosure is not only direct but also mediated by the presence of an SC.Research limitations/implicationsCompanies need to consider the role of women in enhancing the effectiveness of their governance mechanisms and their ability to meet stakeholder information needs. Establishing a specific SC represents a valid mechanism that ensures greater transparency about corporate actions tackled to contribute toward SDGs and enhances the relationship between board gender diversity and SDG disclosure among International companies.Practical implicationsThe study's findings offer stimuli for policy-makers and regulators to reflect on the relevance of the CoP as a possible alternative communication tool to provide SDGs information and overcome the limitations of the Sustainability Reports.Originality/valueThis is the first study that examines companies' SDG disclosure practices focusing on CoPs. Further, to the best of the authors' knowledge, this is the first study that tests the relationship between gender diversity and SDG disclosure, considering the mediating effect of an SC committee.
Local infrastructure, rural households' resilience capacity and poverty: evidence from panel data for Southeast Asia
Authors: Hartwig Tim , Nguyen Trung Thanh 
Published: December 2022

PurposeThe authors examine the association between infrastructure and a household's resilience capacity against shocks and the impacts of a household's resilience capacity on household consumption and poverty.Design/methodology/approachThe authors use panel data (collected in 2010, 2013 and 2016) from 1,698 households in Thailand and 1,701 households in Vietnam and employ an instrumental variable approach.FindingsThe authors find that transportation and information and communication technology (ICT) infrastructure help improve households' absorptive capacity in coping with shocks. Furthermore, this capacity can prevent households from reducing consumption and falling into poverty.Practical implicationsRural development policies should attend to transportation and ICT infrastructure.Originality/valueThe authors establish empirical evidence on the association between infrastructure and a household's resilience capacity and the impact of resilience capacity on poverty.
The link between workplace incivility, emotional exhaustion, and job embeddedness: examining the moderating role of power distance
Authors: Gustiawan Deni , Noermijati Noermijati , Aisjah Siti , Indrawati Nur Khusniyah , Hendryadi Hendryadi 
Published: December 2022

PurposeIntegrating the conservation of resources theory, Hofstede's national culture theory and the cognitive-motivational-relational theory of emotions, the authors propose that power distance (as a moderator) and emotional exhaustion (as a mediator) play a role in the relationship between workplace incivility, emotional exhaustion and job embeddedness.Design/methodology/approachThe data were collected in two stages using an online survey of 404 employees from three sectors, including hospitality, banking and manufacturing, in Indonesia. The authors tested a moderated mediation model using Hayes' macro PROCESS version 3.5.FindingsWorkplace incivility contributes to emotional exhaustion, which predicts job embeddedness. Emotional exhaustion also contributes to job embeddedness. In the moderation model, the authors found that the effect of workplace incivility on emotional exhaustion was more substantial for employees with high perceived power distance. Furthermore, power distance also played a moderating role in the relationship between emotional exhaustion and job embeddedness.Practical implicationsSince workplace incivility and job embeddedness differ across cultures, the results of this study contain practical management implications for Indonesian settings, especially the hospitality, manufacturing and banking sectors. The authors provide practical management implications for redesigning organizational culture to help employees avoid uncivil interactions in the workplace. The authors also provide implications concerning strategic managerial directions to improve communication and supervisors' skills at all levels of management.Originality/valueThis study is the first to introduce power distance as a complementary explanation for the relationship between workplace incivility, emotional exhaustion and job embeddedness while focusing on an Asian developing country.