bluuebunny/crossref_metadata_2025_split huggingface dataset:
TINJAUAN PERENCANAAN PROYEK PEMBANGUNAN GEDUNG / RUANG BARU PUSKESMAS MANDIANGIN KOTA BUKITTINGGI
Authors: Irfan Muhammad , Ishak Ishak , Eka Priana Surya 
Published: June 2023

In general the upper structure includes beams, columns, floor plates and roofs that serve to support the loads that work on a building. The calculations that will be carried out on the construction of Building / New Room of Served Medicine Puskesmas District Mandiangin Bukittinggi in 2021 are floor plates, beams and columns. Structural expenses (dead loads, life loads) are guided by SNI 03-2847:2002 and earthquake loads are guided by SNI 03-1726:2012, where portals are calculated and modeled using the ETABS 2018 program. The purpose of this calculation is to get an idea of the basic principles of planning and reviewing the structure of the building, comparing the results of the calculation of the author with the results of the calculation of the planner consultant. From the calculation results obtained the largest moment on the B1 beam of 131.8420 kN-m and the largest moment in column K1 (50 x 30 cm) of 194,827 kN-m. For floor plates there is a repeating difference: 120 mm thick floor plate, ø 10- 160 mm for direction x and direction y author, floor plate 120 mm, ø 10- 250 mm for direction x and direction y planner, column K1 (50 x 30 cm) 16 D 19 and planner has 16 D 16, for column K2 there are differences in dimensions and bones i.e. : Column K2 (45 x 25 cm) 16 D 19 author, column K2(50 x 30cm) 16D 16 planner. For B1 and B2 blocks there are differences in dimensions and bones namely: beam B1 (45 x 25 cm) 6 D 19 authors, B1 beam (50 x 30 cm) 5 D 19 planner, B2 beam (40 x 25 cm) 6 D 19 author, B2 beam (45 x20 cm) 4 D 19 planner. Keywords : Column Structure, Beam, Floor Plate, Charging, ETABS2018, Reinforcement
PENGARUH PENAMBAHAN LIMBAH BOTOL KACA TERHADAP DAYA SERAP AIR DAN UJI KUAT TEKAN BATU BATA MERAH
Authors: Ari Winata Al Havis , Ishak Ishak , Susanti Yusman Ana 
Published: June 2023

The background of the author doing this research is to improve the construction of houses at this time. This study aims to determine the benefits and strength of ordinary red bricks without mixing with red bricks that have been mixed with glass bottle waste and also to determine the value of water absorption and the value of the compressive strength of red bricks that have been added to a mixture of glass bottle waste. The research that the author did here made samples of bricks and tested water absorption, compressive strength, and made samples in a brick factory located in Manggis Village, Mandiangin Koto Selayan District, Bukittinggi City, as well as testing samples in the laboratory of the Faculty of Engineering, Muhammadiyah University, West Sumatra. In collecting the data needed by the author is a direct survey of the spaciousness and testing the water absorption and compressive strength in the laboratory. In the mixing method, the percentages are 0%, 2%, 4%, 6% and 8%. From the results of research conducted adding glass bottle powder with mixed percentages of 2%, 4%, 6% and 8% can affect the value of water absorption and compressive strength of red bricks, a mixture of 4% has low compressive strength and sufficient water absorption. high so that it is not possible to use in the construction sector. However, the 8% mixture has a higher number than other mixtures and is close to a 0% mixture, a mixture of 8% and 6% red bricks can still be used in the construction sector because the results of water absorption and compressive strength are still close to SNI 15-2094-200 . Keywords: Red brick, Glass Powder, water absorption, compressive strength
Comparison of phasor analysis and biexponential decay curve fitting of autofluorescence lifetime imaging data for machine learning prediction of cellular phenotypes
Authors: Hu Linghao , Ter Hofstede Blanche , Sharma Dhavan , Zhao Feng , Walsh Alex J. 
Published: June 2023

Introduction: Autofluorescence imaging of the coenzymes reduced nicotinamide (phosphate) dinucleotide (NAD(P)H) and oxidized flavin adenine dinucleotide (FAD) provides a label-free method to detect cellular metabolism and phenotypes. Time-domain fluorescence lifetime data can be analyzed by exponential decay fitting to extract fluorescence lifetimes or by a fit-free phasor transformation to compute phasor coordinates.Methods: Here, fluorescence lifetime data analysis by biexponential decay curve fitting is compared with phasor coordinate analysis as input data to machine learning models to predict cell phenotypes. Glycolysis and oxidative phosphorylation of MCF7 breast cancer cells were chemically inhibited with 2-deoxy-d-glucose and sodium cyanide, respectively; and fluorescence lifetime images of NAD(P)H and FAD were obtained using a multiphoton microscope.Results: Machine learning algorithms built from either the extracted lifetime values or phasor coordinates predict MCF7 metabolism with a high accuracy (∼88%). Similarly, fluorescence lifetime images of M0, M1, and M2 macrophages were acquired and analyzed by decay fitting and phasor analysis. Machine learning models trained with features from curve fitting discriminate different macrophage phenotypes with improved performance over models trained using only phasor coordinates.Discussion: Altogether, the results demonstrate that both curve fitting and phasor analysis of autofluorescence lifetime images can be used in machine learning models for classification of cell phenotype from the lifetime data.
RNA-binding proteins that are highly expressed and enriched in healthy cartilage but suppressed in osteoarthritis
Authors: Swahn Hannah , Olmer Merissa , Lotz Martin K. 
Published: June 2023

Objectives: RNA-binding proteins (RBPs) have diverse and essential biological functions, but their role in cartilage health and disease is largely unknown. The objectives of this study were (i) map the global landscape of RBPs expressed and enriched in healthy cartilage and dysregulated in osteoarthritis (OA); (ii) prioritize RBPs for their potential role in cartilage and in OA pathogenesis and as therapeutic targets.Methods: Our published bulk RNA-sequencing (RNA-seq) data of healthy and OA human cartilage, and a census of 1,542 RBPs were utilized to identify RBPs that are expressed in healthy cartilage and differentially expressed (DE) in OA. Next, our comparison of healthy cartilage RNA-seq data to 37 transcriptomes in the Genotype-Tissue Expression (GTEx) database was used to determine RBPs that are enriched in cartilage. Finally, expression of RBPs was analyzed in our single cell RNA-sequencing (scRNA-seq) data from healthy and OA human cartilage.Results: Expression of RBPs was higher than nonRBPs in healthy cartilage. In OA cartilage, 188 RBPs were differentially expressed, with a greater proportion downregulated. Ribosome biogenesis was enriched in the upregulated RBPs, while splicing and transport were enriched in the downregulated. To further prioritize RBPs, we selected the top 10% expressed RBPs in healthy cartilage and those that were cartilage-enriched according to GTEx. Intersecting these criteria, we identified Tetrachlorodibenzodioxin (TCDD) Inducible Poly (ADP-Ribose) Polymerase (TIPARP) as a candidate RBP. TIPARP was downregulated in OA. scRNA-seq data revealed TIPARP was most significantly downregulated in the “pathogenic cluster”.Conclusion: Our global analyses reveal expression patterns of RBPs in healthy and OA cartilage. We also identified TIPARP and other RBPs as novel mediators in OA pathogenesis and as potential therapeutic targets.
No causal genetic relationships between atrial fibrillation and vascular dementia: A bidirectional Mendelian randomization study
Authors: Gao Ya-fang , Jin Tian-yu , Chen Yan , Ding Ya-hui 
Published: June 2023

BackgroundNumerous observational studies have suggested that atrial fibrillation (AF) was associated with an increased risk of vascular dementia (VaD). However, the causal genetic relationships between AF and VaD remains unclear. To evaluate the effect of AF on VaD, we performed the Mendelian randomization (MR) analysis to investigate the causal genetic relationships between AF and VaD.MethodsThe bidirectional MR analysis was conducted to explore the causal relationships between exposure and disease. We applied a series of quality assessments to select significantly and independently single nucleotide polymorphisms (SNPs) from publicly available large-scale genome-wide association studies (GWAS) databases. Three methods [Inverse variance weighted method (IVW), MR-Egger method, and weighted median (WM)method] were used to derive MR estimates. In order to ensure reliable MR results, sensitivity analyses were performed to evaluate the horizontal pleiotropy and heterogeneity.ResultsOur MR analyses revealed no significant genetic relationships between AF and the risk of VaD (IVW: OR =  1.10, 95%CI = 0.95–1.28, P = 0.20). In the reverse direction analysis, there was no evidence to support a significant genetic relationship of VaD with AF risk (IVW: OR =  1.00, 95% CI = 0.99–1.01, P = 0.52). Consistent results were obtained using different MR methods. Sensitivity analyses suggested no significant horizontal pleiotropy and heterogeneity in the study.ConclusionThis MR analysis did not provide evidence to support the causal genetic relationships between AF on VaD risk and the causal effect of VaD on AF risk.
Influence of alpine meadow deterioration on soil microbial communities in the Yangtze River source region
Authors: Zhang Wenjuan 
Published: June 2023

Introduction: Although soil microbial populations are a good predictor of soil texture, little is known about how they react to alpine meadow deterioration.Methods: This study utilized Illumina HiSeq sequencing to investigate the effects of alpine meadow degradation on soil microbial communities in the Yangtze River source basin at five different degradation levels [i.e., non–degraded (ND), slightly degraded (LD), moderately degraded (MD), severely degraded (SD), and very severely degraded (VD)].Results: The results indicated that bacterial and fungal α-diversity were not substantially different (p &gt; 0.05) across the damaged alpine meadows, while β-diversity significantly differed (p &lt; 0.01), indicating a higher variation in the microbial community due to alpine meadow degradation. Proteobacteria reduced considerably (p &lt; 0.05) by 8.75%, 22.99%, and 24.65%, while Acidobacteria increased significantly (p &lt; 0.05) by 41.67%, 85.20%, and 108.67%, in MD, SD, and VD compared with ND, respectively. Ascomycota declined significantly (p &lt; 0.05) in the MD, whereas unclassified phyla rose significantly (p &gt; 0.05) in the VD compared to the ND. The heatmaps of bacterial and fungal communities revealed two clusters: a ND, LD, and MD group and a SD and VD group, suggesting significant changes in soil microorganisms of alpine meadow in the SD and VD. Redundancy analysis (RDA) revealed that soil moisture, soil bulk density, soil organic carbon, total nitrogen, and plant biomass could explain 73.8% and 39.4% of the variance in bacterial and fungal community structure, respectively.Discussion: These findings imply that degradation of the alpine meadow impacts both plant and soil qualities, ultimately leading to changes in soil microbial populations in the Yangtze River’s source region.
Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda
Authors: Bugeza James , Roesel Kristina , Moriyon Ignacio , Mugizi Denis , Alinaitwe Lordrick , Kivali Velma , Kankya Clovice , Cook Elizabeth Anne Jessie 
Published: June 2023

IntroductionBrucellosis is endemic in Uganda and is a major cause of production losses in livestock. Early detection and quantification of the disease is vital for its control and eradication. The aim of this study was to assess the sero-prevalence and factors associated with anti-Brucella antibodies in slaughtered livestock.Materials and methodsSera from 886 cattle, 925 small ruminants, and 900 pigs were collected from regional abattoirs in Northern, Eastern and Central Uganda. To estimate sero-prevalence, sera were serially tested using a combination of the Rose Bengal Test (RBT) and Native Hapten (NH) immunoprecipitation test. True sero-prevalence was estimated using the Rogan-Gladden estimator considering the sensitivity and specificity of the NH immunoprecipitation test. Multivariable logistic regression was used to assess factors associated with seropositivity for anti-Brucella antibodies.Results and discussionSmall ruminants showed the highest seroprevalence (6.7%, 95% CI = 4.2-7.1) followed by cattle (3.8%, 95% CI = 2.4-4.9) and pigs (2.8%, 95% CI = 1.1-2.9). Seropositivity for anti-Brucella antibodies was associated with region of origin (OR = 4.6,95%CI=1.49-17.75, p = 0.013) for cattle; sex (OR = 2.90, 95% C = 1.5-6.34, p = 0.004), age (OR=4.04, 95% CI = 1.07-8.52, p = 0.006) and species (OR = 2.53, 95% CI = 1.08-6.98, p = 0.048) for small ruminants; and finally sex for pigs (OR = 2.88, 95% CI = 1.07-8.52, p = 0.041). Progressive control interventions must include both cattle and small ruminants since they play a bigger role in the maintenance and dissemination of Brucella. The interventions should adopt a risk-based approach with regions at higher risk being given top priority. Bacteriological and molecular studies should be undertaken to clarify the role of pigs and the goat-cattle cross infections in the epidemiological cycle of brucellosis in Uganda.
PARP2 downregulation in T cells ameliorates lipopolysaccharide-induced inflammation of the large intestine
Authors: Bencsics Máté , Bányai Bálint , Ke Haoran , Csépányi-Kömi Roland , Sasvári Péter , Dantzer Françoise , Hanini Najat , Benkő Rita , Horváth Eszter M. 
Published: June 2023

IntroductionT cell-dependent inflammatory response with the upregulation of helper 17 T cells (Th17) and the downregulation of regulatory T cells (Treg) accompanied by the increased production of tumor necrosis alpha (TNFa) is characteristic of inflammatory bowel diseases (IBD). Modulation of T cell response may alleviate the inflammation thus reduce intestinal damage. Poly(ADP-ribose) polymerase-2 (PARP2) plays role in the development, differentiation and reactivity of T cell subpopulations. Our aim was to investigate the potential beneficial effect of T cell-specific PARP2 downregulation in the lipopolysaccharide (LPS) induced inflammatory response of the cecum and the colon.MethodsLow-dose LPS was injected intraperitoneally to induce local inflammatory response, characterized by increased TNFa production, in control (CD4Cre; PARP2+/+) and T cell-specific conditional PARP2 knockout (CD4Cre; PARP2f/f) mice. TNFa, IL-1b, IL-17 levels were measured by ELISA, oxidative–nitrative stress was estimated by immunohistochemistry, while PARP1 activity, p38 MAPK and ERK phosphorylation, and NF-kB expression in large intestine tissue samples were examined by Western-blot. Systemic &amp; local T cell subpopulation; Th17 and Treg alterations were also investigated using flowcytometry and immunohistochemistry.ResultsIn control animals, LPS induced intestinal inflammation with increased TNFa production, while no significant elevation of TNFa production was observed in T cell-specific PARP2 knockout animals. The absence of LPS-induced elevation in TNFa levels was accompanied by the absence of IL-1b elevation and the suppression of IL-17 production, showing markedly reduced inflammatory response. The increase in oxidative-nitrative stress and PARP1-activation was also absent in these tissues together with altered ERK and NF-kB activation. An increase in the number of the anti-inflammatory Treg cells in the intestinal mucosa was observed in these animals, together with the reduction of Treg count in the peripheral circulation.DiscussionOur results confirmed that T cell-specific PARP2 downregulation ameliorated LPS-induced colitis. The dampened TNFa production, decreased IL-17 production and the increased intestinal regulatory T cell number after LPS treatment may be also beneficial during inflammatory processes seen in IBD. By reducing oxidative-nitrative stress and PARP1 activation, T cell-specific PARP2 downregulation may also alleviate intestinal tissue damage.
Analysis of a series of Italian APECED patients with autoimmune hepatitis and gastro-enteropathies
Authors: Paldino Giorgia , Faienza Maria Felicia , Cappa Marco , Pietrobattista Andrea , Capalbo Donatella , Valenzise Mariella , Lampasona Vito , Cudini Annamaria , Carbone Elena , Pagliarosi Olivia , Maggiore Giuseppe , Salerno Mariacarolina , Betterle Corrado , Fierabracci Alessandra 
Published: June 2023

IntroductionAutoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) syndrome is a rare monogenic disease determined by biallelic mutations in AIRE gene, which encodes a transcription factor essential for central immune tolerance. Classic diagnosis is determined by the presence of two of the main APECED clinical diseases: chronic mucocutaneous candidiasis, chronic hypoparathyroidism, and Addison’s disease. Non-endocrine autoimmunity, involving the liver, intestine, eyes, and kidneys, is generally reported in a minority of European patients, while American APECED patients have a higher tendency of developing organ-specific non-endocrine manifestations early in life. This observation led to the revision of the diagnostic criteria to permit earlier diagnosis based on the appearance of one classic triad symptom or one non-classical manifestation at a young age in the presence of IFNωAbs or AIRE mutations (Ferre-Lionakis criteria).Patients and methodsWe analyzed the clinical, genetic, and autoantibody (Ab) profiles in a series of 14 pediatric Italian APECED patients with gastrointestinal manifestations (seven male and seven female patients). Ten patients presented hepatitis (APECED-associated hepatitis (APAH)), while seven were affected by constipation, diarrhea, and malabsorption. Four patients had developed APAH before classic triad symptoms.ResultsBased on the age of appearance of non-endocrine manifestations including APAH and gastro-enteropathy, the Ferre-Lionakis criteria would have allowed an expedited diagnosis in 11/14 patients. Abs to tryptophan hydroxylase (TPHAb) and hepatic aromatic l-amino acid decarboxylase (AADC) were significantly associated with APECED patients of the present series. Abs to cP4501A2 were detectable in the serum of 4/8 patients with APAH, and Abs to cP4502A6 were detectable in 3/8 patients. AADC Abs tested positive in 5/7 patients, which is indicative of gastrointestinal dysfunction in APECED and TPHAb in 5/7 patients with gastrointestinal dysfunction. IFNAb was significantly associated with the syndrome.ConclusionAlthough Ferre-Lionakis expanded criteria applied to the American cohorts of APECED patients would require validation in independent large cohorts of European patients, the results of this study emphasize the importance to evaluate the presence and the age of appearance of APAH and autoimmune enteropathy even in European cohorts for an earlier APECED diagnosis. An earlier APECED diagnosis would also allow the prevention of episodes of life-threatening hypocalcemic seizures and adrenal crisis, which are the main manifestations of undiagnosed APECED.
Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins
Authors: Xu Yaozheng , Huo Jiawen , Nie Ruili , Ge Lili , Xie Chonghong , Meng Yuan , Liu Jianhua , Wu Lina , Qin Xiaosong 
Published: June 2023

ObjectivesHashimoto’s thyroiditis (HT) is one of the most common autoimmune disorders; however, its underlying pathological mechanisms remain unclear. Although aberrant glycosylation has been implicated in the N-glycome of immunoglobulin G (IgG), changes in serum proteins have not been comprehensively characterized. This study aimed to investigate glycosylation profiles in serum samples depleted of highly abundant proteins from patients with HT and propose the potential functions of glycoproteins for further studies on the pathological mechanisms of HT.MethodsA lectin microarray containing 70 lectins was used to detect and analyze glycosylation of serum proteins using serum samples (N=27 HT; N=26 healthy control [HC]) depleted of abundant proteins. Significant differences in glycosylation status between HT patients and the HC group were verified using lectin blot analysis. A lectin-based pull-down assay combined with mass spectrometry was used to investigate potential glycoproteins combined with differentially present lectins, and an enzyme-linked immunosorbent assay (ELISA) was used to identify the expression of targeted glycoproteins in 131 patients with papillary thyroid carcinoma (PTC), 131 patients with benign thyroid nodules (BTN) patients, 130 patients with HT, and 128 HCs.ResultsCompared with the HC group, the majority of the lectin binding signals in HT group were weakened, while the Vicia villosa agglutinin (VVA) binding signal was increased. The difference in VVA binding signals verified by lectin blotting was consistent with the results of the lectin microarray. A total of 113 potential VVA-binding glycoproteins were identified by mass spectrometry and classified by gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analyses. Using ELISA, we confirmed that lactoferrin (LTF) and mannan-binding lectin-associated serine protease 1 (MASP-1) levels were elevated in the serum of patients with HT and PTC.ConclusionFollowing depletion of abundant proteins, remaining serum proteins in HT patients exhibited lower glycosylation levels than those observed in HCs. An increased level of potential VVA-binding glycoproteins may play an important role in HT development. LTF and MASP-1 expression was significantly higher in the serum of HT and PTC patients, providing novel insight into HT and PTC.