bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of living alone with clinical outcomes in patients with heart failure: A systematic review and meta‐analysis
Authors: Chen Haibo , Liu Fuwei , Luo Jun , Tu Yating , Huang Shan , Zhu Wengen 
Published: September 2023

AbstractLiving alone is an objective sign of social isolation. It is uncertain whether living alone worsens clinical outcomes in heart failure (HF) patients. We aimed to assess how living alone affected clinical outcomes in individuals with HF. We searched the electronic databases of PubMed, Embase, and Cochrane from 1990 to April 2022 for studies comparing living alone with HF. A random‐effects model with inverse variance was used to pool adjusted hazard ratios (HRs) and 95% confidence intervals (CIs). Seven studies were deemed to meet the standards. In patients with HF, compared with living with others, living alone was associated with an elevated risk of any hospitalization at the 30‐day (HR: 1.78, 95% CI: 1.09–2.89), 90‐day (HR: 1.24, 95% CI: 1.02–1.51), or ≥1‐year (HR: 1.14, 95% CI: 1.04–1.26) follow‐up periods. HF patients living alone also had a greater risk of any hospitalization or death at the 30‐day (HR: 1.56, 95% CI: 1.15–2.11), 90‐day (HR: 1.26, 95% CI: 1.05–1.50), and ≥1‐year (HR: 1.18, 95% CI: 1.09–1.28) follow‐up periods. However, patients living alone had no increased risk of all‐cause death at the 30‐day (HR: 1.0, 95% CI: 0.19–5.36), 90‐day (HR: 0.46, 95% CI: 0.03–7.42), or ≥ 1‐year (HR: 1.10, 95% CI: 0.73–1.67) follow‐up periods. In comparison to living with others, living alone was associated with an increased risk of any hospitalization but not all‐cause death in HF patients.
Fluorescent‐guided surgery and the use of indocyanine green sentinel lymph node mapping in the pediatric and young adult oncology population
Authors: Johnston Michael E. , Farooqui Zishaan A. , Nagarajan Rajaram , Pressey Joseph G. , Turpin Brian , Dasgupta Roshni 
Published: September 2023

AbstractBackgroundTechnetium‐99 (99mTc) lymphoscintigraphy with blue dye injection is an accepted method for sentinel lymph node (SLN) mapping, but blue dye has known adverse effects, and injection of 99mTc may increase time under anesthesia for pediatric patients. Indocyanine green (ICG) may serve as an adjunct to assist with visibility and identification of SLNs. We hypothesized that sensitivity of ICG was similar to blue dye in SLN biopsies.MethodsThirty patients (36 procedures with 96 total specimens) underwent preoperative intradermal injection of 99mTc, followed by intradermal injection of isosulfan blue and ICG. Test characteristics of blue dye, ICG, and 99mTc included sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV).ResultsICG had a sensitivity of 87% and PPV of 83% for detection of 99mTc‐hot lymph nodes; blue dye had a sensitivity of 44% and PPV of 97%. For detection of pathologically confirmed lymph nodes, ICG had a sensitivity of 84% and a positive predictive value (PPV) of 91%. 99mTc had a sensitivity of 82% and a PPV of 94%. ICG had no significant difference in odds of being positive in pathology‐confirmed lymph nodes compared to 99mTc (odds ratio [OR], 0.818; 95% confidence interval [CI], 0.3–2.172; p = .823) and had higher odds than isosulfan blue (OR, 0.025, 95% CI, 0.001–0.148; p < .001).ConclusionThis study established the efficacy of ICG as an adjunct to SLNB in the pediatric and young adult population. ICG was safe, more efficacious than blue dye, and may obviate the need for lymphoscintigraphy in selected patients resulting in reduced time under anesthesia.
Glu‐derived Zwitterionic Micelles Conquering the Intestinal Mucosal Barrier toward Oral Delivery
Authors: Liu Kedong , Chen Yun , Yang Dutao , Cai Yanfei , Yang Zhaoqi , Jin Jian 
Published: September 2023

AbstractAmino acid transporters with different categories are highly expressed in intestinal epithelial cells for maintaining the essential metabolism of body. The zwitterionic property of amino acid with α‐amino and α‐carboxyl also has tremendous potential to conquer the mucus barrier. Therefore, Glu‐derived amphiphilic block polymers [i. e., poly(lactic acid)‐b‐poly(glutamine)25 (PPQ) and poly(lactic acid)‐b‐poly(glutamate)25 (PPD)] were rational designed and Glu‐derived zwitterionic micelles (PPQ‐M and PPD‐M) were further prepared toward oral delivery. In comparison with PEGylated micelles (PEG‐M), PPD‐M and PPQ‐M possessed superior mucus permeability. In addition, enhanced cellular internalization capacity was also detected in both zwitterionic micelles, which was mainly mediated by multiple amino acid transporters. The cellular uptake efficiency of PPQ‐M was obviously higher than that of PPD‐M due to the glutamine constitutional unit of PPQ‐M with higher affinity to amino acid transporters. Furthermore, the retrograde pathway played an important role in the intracellular transport (including transcytosis) of both zwitterionic micelles. Excellent villi absorption of zwitterionic micelles was observed in situ, which gave a convincing evidence for oral delivery application prospect. The results of this research demonstrated that Glu‐derived zwitterionic micelles with distinguished capacity of conquering intestinal mucosal barrier, have promising potential toward oral delivery.
Targeted demethylation of the CDO1 promoter based on CRISPR system inhibits the malignant potential of breast cancer cells
Authors: Yang Jiaojiao , Sun Liyue , Liu Xiao‐Yun , Huang Chan , Peng Junling , Zeng Xinxin , Zheng Hailin , Cen Wenjian , Xu Yu‐Xia , Zhu Weijie , Wu Xiao‐Yan , Ling Dongyi , Zhang Lu‐Lu , Wei Mingbiao , Liu Ye , Wang Deshen , Wang Feng‐Hua , Li Yu‐Hong , Li Qin , Du Ziming 
Published: September 2023

AbstractBackgroundCysteine dioxygenase 1 (CDO1) is frequently methylated, and its expression is decreased in many human cancers including breast cancer (BC). However, the functional and mechanistic aspects of CDO1 inactivation in BC are poorly understood, and the diagnostic significance of serum CDO1 methylation remains unclear.MethodsWe performed bioinformatics analysis of publicly available databases and employed MassARRAY EpiTYPER methylation sequencing technology to identify differentially methylated sites in the CDO1 promoter of BC tissues compared to normal adjacent tissues (NATs). Subsequently, we developed a MethyLight assay using specific primers and probes for these CpG sites to detect the percentage of methylated reference (PMR) of the CDO1 promoter. Furthermore, both LentiCRISPR/dCas9‐Tet1CD‐based CDO1‐targeted demethylation system and CDO1 overexpression strategy were utilized to detect the function and underlying mechanism of CDO1 in BC. Finally, the early diagnostic value of CDO1 as a methylation biomarker in BC serum was evaluated.ResultsCDO1 promoter was hypermethylated in BC tissues, which was related to poor prognosis (p < .05). The CRISPR/dCas9‐based targeted demethylation system significantly reduced the PMR of CDO1 promotor and increased CDO1 expression in BC cells. Consequently, this leads to suppression of cell proliferation, migration and invasion. Additionally, we found that CDO1 exerted a tumour suppressor effect by inhibiting the cell cycle, promoting cell apoptosis and ferroptosis. Furthermore, we employed the MethyLight to detect CDO1 PMR in BC serum, and we discovered that serum CDO1 methylation was an effective non‐invasive biomarker for early diagnosis of BC.ConclusionsCDO1 is hypermethylated and acts as a tumour suppressor gene in BC. Epigenetic editing of abnormal CDO1 methylation could have a crucial role in the clinical treatment and prognosis of BC. Additionally, serum CDO1 methylation holds promise as a valuable biomarker for the early diagnosis and management of BC.
Minimal residual disease assessment in B‐cell precursor acute lymphoblastic leukemia by semi‐automated identification of normal hematopoietic cells: A EuroFlow study
Authors: Verbeek Martijn W. C. , Rodríguez Beatriz Soriano , Sedek Lukasz , Laqua Anna , Buracchi Chiara , Buysse Malicorne , Reiterová Michaela , Oliveira Elen , Morf Daniela , Oude Alink Sjoerd R. , Barrena Susana , Kohlscheen Saskia , Nierkens Stefan , Hofmans Mattias , Fernandez Paula , de Costa Elaine Sobral , Mejstrikova Ester , Szczepanski Tomasz , Slota Lukasz , Brüggemann Monika , Gaipa Giuseppe , Grigore Georgiana , van Dongen Jacques J. M. , Orfao Alberto , van der Velden Vincent H. J. 
Published: September 2023

AbstractPresence of minimal residual disease (MRD), detected by flow cytometry, is an important prognostic biomarker in the management of B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL). However, data‐analysis remains mainly expert‐dependent. In this study, we designed and validated an Automated Gating & Identification (AGI) tool for MRD analysis in BCP‐ALL patients using the two tubes of the EuroFlow 8‐color MRD panel. The accuracy, repeatability, and reproducibility of the AGI tool was validated in a multicenter study using bone marrow follow‐up samples from 174 BCP‐ALL patients, stained with the EuroFlow BCP‐ALL MRD panel. In these patients, MRD was assessed both by manual analysis and by AGI tool supported analysis. Comparison of MRD levels obtained between both approaches showed a concordance rate of 83%, with comparable concordances between MRD tubes (tube 1, 2 or both), treatment received (chemotherapy versus targeted therapy) and flow cytometers (FACSCanto versus FACSLyric). After review of discordant cases by additional experts, the concordance increased to 97%. Furthermore, the AGI tool showed excellent intra‐expert concordance (100%) and good inter‐expert concordance (90%). In addition to MRD levels, also percentages of normal cell populations showed excellent concordance between manual and AGI tool analysis. We conclude that the AGI tool may facilitate MRD analysis using the EuroFlow BCP‐ALL MRD protocol and will contribute to a more standardized and objective MRD assessment. However, appropriate training is required for the correct analysis of MRD data.
Immunoreactivity of TTF‐1, GATA‐3, CEA, and p16/Ki67 cocktail in Cellprep®‐processed control samples: Comparison of long‐term storage in vials and slides
Authors: Ryu Ayumi , Honma Keiichiro , Umeno Yoshimi , Uefuji Fusayo , Muramatsu Takako , Nishimura Sanako , Tanada Satoshi , Yamamoto Takashi , Nagata Shigenori , Yamasaki Tomoyuki , Ohue Masayuki 
Published: September 2023

AbstractIntroductionCellprep® (CP) is a novel liquid‐based cytology (LBC) system. This study aimed to assess the immunoreactivity of control samples stored long‐term under two storage conditions using CP.MethodsThe immunoreactivity in control samples was evaluated under two sample storage conditions: CP vial storage at room temperature (RT: 20–25°C) and CP slide storage in a refrigerator (2–6°C). Clinical samples as controls (total: positive/negative) were immunostained using thyroid transcription factor‐1 (TTF‐1) (20: 14/6), GATA binding protein 3 (GATA‐3) (13: 10/3), carcinoembryonic antigen (CEA) (23: 15/8), and the p16/Ki67 cocktail (20: 12/8) markers. The first immunocytochemistry (ICC) was performed within 1 month using CP vials stored at RT. Samples stored in CP vials and on CP slides were used for the second (within 3–6 months) and third (within 6–11 months) ICCs. Compared with the first ICC, the concordance of immunoreactivity for the second and third ICCs was evaluated using the weighted kappa coefficient.ResultsFor TTF‐1, CEA, and the p16/Ki67 cocktail markers, ICC controls had stable immunoreactivity for a minimum of 6 months when samples were stored in CP vials (kappa coefficients >0.8), whereas for GATA‐3, they were for 3 months. On CP slides, only for the p16/Ki67 cocktail, ICC controls had stable immunoreactivity for at least 3 months (kappa coefficient >0.8).ConclusionClinical samples as ICC controls revealed consistently more stable immunoreactivity in CP vials than on CP slides for TTF‐1, GATA‐3, CEA, and the p16/Ki67 cocktail markers.
PRecision Event Monitoring for PatienTs with Heart Failure using HeartLogic (PREEMPT‐HF) study design and enrolment
Authors: Boehmer John , Sauer Andrew J. , Gardner Roy , Stolen Craig M. , Kwan Brian , Wariar Ramesh , Ruble Stephen 
Published: September 2023

AbstractAimsThe HeartLogic multisensor index has been found to be a sensitive predictor of worsening heart failure (HF). However, there is limited data on this index's association and its constituent sensors with HF readmissions.Methods and resultsThe PREEMPT‐HF study is a global, multicentre, prospective, observational, single‐arm, post‐market study. HF patients with an implantable defibrillator device or cardiac resynchronization therapy with defibrillator with HeartLogic capabilities were eligible if sensor data collection was turned on and the HeartLogic feature was not enabled. Thus, the HeartLogic Index/alert and heart sounds sensor trends were unavailable via the LATITUDE remote monitoring system to clinicians (blinded). Evaluation of subject medical records at 6 months and a final in‐clinic visit at 12 months was required for collection of all‐cause hospitalizations and HF outpatient visits. The purpose of this study is exploratory, no formal hypothesis tests are planned, and no adjustment for multiple testing will be performed. A total of 2183 patients were enrolled at 103 sites between June 2018 and June 2020. A significant proportion of the patients were implanted with implantable defibrillator devices (39%) versus cardiac resynchronization therapy with defibrillator (61%); were female (27%); over 65 (61%); New York Heart Association class I (13%), II (53%), and III (33%); ejection fraction < 25% (21%); ischaemic (50%); and with a history of renal dysfunction (23%).ConclusionsThe PREEMPT study will provide clinical data and blinded sensor trends for the characterization of sensor changes with HF readmission, tachyarrhythmias, and event subgroups. These data may help to refine the clinical use of HeartLogic and to improve patient outcomes.
From simple to complex: Protein‐based biomarker discovery in tuberculosis
Authors: Mousavian Zaynab , Källenius Gunilla , Sundling Christopher 
Published: September 2023

AbstractTuberculosis (TB) is a deadly infectious disease that affects millions of people globally. TB proteomics signature discovery has been a rapidly growing area of research that aims to identify protein biomarkers for the early detection, diagnosis, and treatment monitoring of TB. In this review, we have highlighted recent advances in this field and how it is moving from the study of single proteins to high‐throughput profiling and from only using proteomics to include additional types of data in multi‐omics studies. We have further covered the different sample types and experimental technologies used in TB proteomics signature discovery, focusing on studies of HIV‐negative adults. The published signatures were defined as either coming from hypothesis‐based protein targeting or from unbiased discovery approaches. The methodological approaches influenced the type of proteins identified and were associated with the circulating protein abundance. However, both approaches largely identified proteins involved in similar biological pathways, including acute‐phase responses and T‐helper type 1 and type 17 responses. By analysing the frequency of proteins in the different signatures, we could also highlight potential robust biomarker candidates. Finally, we discuss the potential value of integration of multi‐omics data and the importance of control cohorts and signature validation.
A Cobalt Complex from Terpyridine‐Based Peptoid as an Efficient Catalyst for Visible Light Driven Water Oxidation
Authors: Pahar Suraj , Majee Karunamay , Maayan Galia 
Published: September 2023

AbstractWe report on the first peptoid‐based catalyst for visible light driven water oxidation, the complex TPT2Co. Peptoids are N‐substituted glycine oligomers, and TPT is a peptoid trimer incorporating terpyridine ligand, an ethanolic group as a proton acceptor and a non‐catalytic benzyl group. We explored the ability of TPT2Co to perform as a catalyst for water oxidation in phosphate buffer at pH 9.5, in the presence of the photosensitizer [Ru(bpy)3]2+ and the sacrificial electron acceptor Na2S2O8. We demonstrated that TPT2Co can catalyze water oxidation by a blue LED with an intensity of only 1.5 mW/cm2 towards oxygen production at pH 9.5 with a maximal TON of 28.1 in 45 min. The stability of TPT2Co during the light‐driven water oxidation as well as its ability to stabilize the photosensitizer were confirmed by a combination of UV‐Vis, IR and ESI‐MS analysis.
Synthetic Approaches to Ribosyl Adenosine 5′,5′′‐Diphosphate Fragment of Poly(ADP‐ribose)
Authors: Hagino Rui , Mozaki Keita , Komura Naoko , Imamura Akihiro , Ishida Hideharu , Ando Hiromune , Tanaka Hide‐Nori 
Published: September 2023

AbstractRibosyl adenosine 5′,5′′‐diphosphate is a poly(ADP‐ribose) fragment covalently bonded to a protein during post‐translational modification. Poly(ADP‐ribose) is involved in several biological processes such as DNA repair and cancerization. Herein, we report the development of two synthetic approaches to the poly(ADP‐ribose) fragment via a common precursor. The major difficulty in the synthesis of the fragment is the α‐(1′′→2′)‐glycosidic bond formation between the ribose and adenosine because of the lack of neighboring group participation and low reactivity of the 2′‐hydroxyl group. The first approach employed an indirect method that involved stepwise assembly of the ribosyl adenosine framework by O‐ribofuranosylation of a commercially available ribose acceptor and subsequent N‐glycosylation of N6‐benzoyl adenine (14 linear longest sequence (LLS) steps, 11 pots, 6.8 % overall yield). In the second approach, the direct O‐ribofuranosylation of a known 6‐chloropurine riboside acceptor was adopted (12 LLS steps, 10 pots, 4.8 % overall yield). Thus, two practical synthetic approaches to the target fragment were successfully established in terms of the number of LLS steps, pots, and overall yield. Furthermore, the precursor was converted into a conjugation‐ready building block, primed for application in ADP‐ribose oligomer synthesis.
Risk‐based optimization of concentrically braced tall timber buildings: Derivative free optimization algorithm
Authors: Laguardia Raffaele , Franchin Paolo , Tesfamariam Solomon 
Published: September 2023

AbstractMass timber materials are attractive alternatives for tall‐timber buildings (TBs), where the need for sustainability is apparent. Innovative structural systems and design methodologies are needed to fulfil performance requirements according to modern performance based approaches. This paper deals with the design and optimization of buckling restrained braces as earthquake protection system for tall‐TBs through risk‐based design procedure. This procedure controls the mean annual frequency of exceedance of several limit states evaluated through a SAC‐FEMA approach and using response spectrum linear analyses on linearized models for demand assessment. The features of the optimization procedure and the linearized models are shown through an application on a 20‐story mass‐TB located in a high seismic zone. The optimization is executed through a derivative‐free algorithm, the generalized pattern Search, adopting several solution strategies whose efficiency and effectiveness for this kind of applications are shown and discussed. Finally, the results are compared and validated through the execution of non‐linear analyses within a multiple stripe framework.
Experimental evaluation of the role of slenderness in seismic behaviour of bridges with rockable footing
Authors: Yang Ziqi , Lyu Yang , Li Ning , Li Zhongxian , Xue Ruisong , Chouw Nawawi 
Published: September 2023

AbstractObservations in major earthquakes have shown that rockable structures suffered less to no damage. During rocking, that is, partial and temporary footing separations, the influx of seismic energy is interrupted and thus the impact of the base excitation is reduced. Rocking causes the structure to deform more rigid like. Consequently, the structure experiences less deformation along the height and thus a lower damage potential. Although many researchers have studied the influence of rockable footings, most of these are either analytical or numerical, and only a very few structures have been built with rockable footings worldwide, for example, the chimney at Christchurch Airport and the South Rangitikei Viaduct in New Zealand. Despite these studies, a thorough and understanding is not yet available, especially with respect to experimental validations. This work is the first to investigate the rocking behaviour of bridges with different slenderness using large‐scale shake table experiments. To limit the number of influence factors, a stiff footing support and the same fixed‐base fundamental frequency of the bridges were assumed. The result shows that the girder displacement and the footing rotation of the tall bridge do not always move in phase, which cannot be observed in the short bridge. The results demonstrate the important role of slenderness in the overall responses of rockable bridges. This behaviour cannot be observed in bridges with a commonly assumed fixed base since the slenderness effect cannot be activated.
Crop models and their use in assessing crop production and food security: A review
Authors: Gavasso‐Rita Yohanne Larissa , Papalexiou Simon Michael , Li Yanping , Elshorbagy Amin , Li Zhenhua , Schuster‐Wallace Corinne 
Published: September 2023

AbstractAgriculture is directly related to food security as it determines the global food supply. Research in agriculture to predict crop productivity and losses helps avoid high food demand with little supply and price spikes. Here, we review ten crop models and one intercomparison project used for simulating crop growth and productivity under various impacts from soil–crop–atmosphere interactions. The review outlines food security and production assessments using numerical models for maize, wheat, and rice production. A summary of reviewed studies shows the following: (1) model ensembles provide smaller modeling errors compared to single models, (2) single models show better results when coupled with other types of models, (3) the ten reviewed crop models had improvements over the years and can accurately predict crop growth and yield for most of the locations, management conditions, and genotypes tested, (4) APSIM and DSSAT are fast and reliable in assessing broader output variables, (5) AquaCrop is indicated to investigate water footprint, quality and use efficiency in rainfed and irrigated systems, (6) all models assess nitrogen dynamics and use efficiency efficiently, excluding AquaCrop and WOFOST, (7) JULES specifies in evaluating food security vulnerability, (8) ORYZA is the main crop model used to evaluate paddy rice production, (9) grain filling is usually assessed with APSIM, DAISY, and DSSAT, and (10) the ten crop models can be used as tools to evaluate food production, availability, and security.