bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure of full-length cobalamin-dependent methionine synthase and cofactor loading captured in crystallo
Authors: Koutmos Markos , Mendoza Johnny , Purchal Meredith , Yamada Kazuhiro 
Published: June 2023

Abstract
Cobalamin-dependent methionine synthase (MS) is a key enzyme in methionine and folate one-carbon metabolism. MS is a large multi-domain protein capable of binding and activating three substrates: homocysteine, folate, S-adenosylmethionine for methylation. Achieving three chemically distinct methylations necessitates significant domain rearrangements to facilitate substrate access to the cobalamin cofactor at the right time. The distinct conformations required for each reaction have eluded structural characterization as its inherently dynamic nature renders structural studies difficult. Here, we use a thermophilic MS homolog (tMS) as a functional MS model. Its exceptional stability enabled characterization of MS in the absence of cobalamin, marking the first studies of a cobalamin-binding protein in its apoenzyme state. More importantly, we report the first high-resolution full-length MS structure, ending a multi-decade quest. We also captured cobalamin loading in crystallo, providing structural insights into holoenzyme formation. Our work paves the way for unraveling how MS orchestrates large-scale domain rearrangements crucial for achieving challenging chemistries.
Single-crystalline LiMn 2 O 4 nanoparticles by the gel-combustion method assisted by microwave for high-performance lithium ion battery
Authors: Qiu Zehai , Huang Tianfu , Hu Zhibiao , Zhang Zhusen 
Published: June 2023

Abstract
Low-cost and efficient electrode materials play a key role in improving the performance of Lithium Ion Battery. In this paper, the single-crystalline LiMn2O4 nanoparticles were synthesized through the gel-combustion method assisted by microwave followed by calcination treatment. High-quality single-crystallinity characteristics of the LiMn2O4 precursors powder could be well retained after a high-temperature (990℃) solid-state reaction. When valued as the working electrode materials for Lithium Ion Battery, they presented the exceptional electrochemical performances including the high specific capacity for initial discharge of 120.53mAh/g, the good rate capability with the retention of 65.52% at 5C of the capacity at 0.2C, and better cyclic performance with a capacity retention ratio of 90.01% after 300 cycles. The outstanding electrochemical performance of our prepared single-crystalline LiMn2O4 nanoparticles was perceived as the hopeful electrode materials for high-power Lithium Ion Battery.
Analytical performance evaluation of Hematology analyzers using Sigma metrics
Authors: Berta Dereje , Melku Mulugeta , Adane Tiruneh , Girma Mekonnen , Mulatie Zewudu , Chane Elias , Birke Bisrat 
Published: June 2023

Abstract
Introduction:
In clinical laboratory, the performance of the hematology analyzers should be checked routinely to ensure that the desired quality is achieved. Therefore, the aim of the study was to assess the performance of hematology analyzers using sigma metrics.
Method
The study included all daily internal quality control data of hematology analyzer prospectively from August to October 2022. Data was collected by trained laboratory professionals using record formats. The sigma values of each CBC parameter were calculated using the formula: Sigma = (TEa – Bias) / CV. The data of TEa were adopted from five different guidelines. The bias of all complete blood count parameters was calculated from the laboratory mean of the daily IQC data and the target value of the manufacturer in the insert kit. A coefficient of variation was also calculated using IQC data.
Results
The current study found an inconsistent sigma value, based on sources TEa. Except HCT out 5 parameters included based on CLIA guideline, except MCV, MCHC, RDW, MPV and Basophil out of 15 parameters included based on EFLM 2022 minimum guideline, except Hb and PLT out of 9 parameters included based on SOTA guideline other parameters meets minimum specification. On the other hand, all parameters included in Rilibak and Standards of Spanish guideline achieved minimum specification.
Conclusion
Sigma values of the CBC parameters have significantly varied depends on the TEa sources. So, the source of TEa needs harmonization. Additionally, suggested that the laboratory to optimize local Westgard rules for each parameter.
Correlation between serum complement and severity of lumbar degenerative disease: A cross-sectional study
Authors: Zhu Xiaoxuan , Cao Yiqiu , Jiang Qing , Gu Yifei , Chen Yu , Chen Thomas C. , Wu Zhourui , Cheng Liming 
Published: June 2023

Abstract
PURPOSE: To investigate the relationship between preoperative serum complement level and the severity of lumbar degenerative disease (LDD), as well as the severity of pain and the improvement rate of surgical symptoms.
METHODS: The serum complements (C3, C4 and C1q) levels were determined by enzyme-linked immunosorbent assay. MRI was used to determine the degree of spinal degeneration. Mann-Whitney non-parametric test and Pearson correlation analysis were performed to predict the distance of relationships.
RESULTS: Compared with the control group, the serum complements levels (C3, C4) of patients with LDD were significantly increased. We observed a significant correlation between the C3 level and the dural sac cross-sectional area (S1) (R = 0.6022, p = 0.0383) and S1/lumbar canal area (S0) (R = 0.6212, p = 0.0311). In addition, a correlation between preoperative serum C1q level and improvement rate of pain (R = -0.6607, p = 0.0140) was observed.
CONCLUSION: The findings showed that serum levels of C3 and C4 were elevated in patients with LDD. Pearson correlation analysis showed that the C3 level could predict the dural sac cross-sectional area and be adopted as a promising predictive biomarker for spinal cord compression. Moreover, preoperative serum C1q concentration could predict the improvement rate of surgical symptoms.
Enhancing Efficiency and Sustainability: A Predictive Modeling Approach Using Extra Tree Regressor for Cement Kiln Cooler Process
Authors: BENCHEKROUN Mohammed Toum , ZAKI Smail , ABOUSSALEH Mohamed , BEKKALI FATIMA ZAHRAE EL , CHERIF Meryem 
Published: June 2023

Abstract
The clinker cooler is an important part of the cement manufacturing process, as it plays a crucial role in controlling the temperature of the clinker leaving the kiln. To optimize the clinker cooler in terms of energy and throughput, this paper presents a methodology based on artificial intelligence and mainly on machine learning, which could revolutionize the cement industry by improving efficiency, reducing costs, and increasing production. The sensor data are used after preprocessing to implement the different models of the supervised regression problem. The models have four output variables which include pressure, temperature, and speed. This aims to identify the right model which is Extra Tree regressor after comparison according to two metrics that are the mean absolute error, and score R2 and to optimize the process parameters in real time.
Single-cell sequencing reveal Renin-Angiotensin-System regulator patterns guide intercellular communication of tumor microenvironment that contribute to gastric cancer progression and immunotherapy
Authors: Xu Qingyu , Xue Shuai , Wang Qiuqiu , Zhang Yaqiong , Li Jian , Zhang Yanyan , Feng Li 
Published: June 2023

Abstract
Background
The renin-angiotensin system (RAS), which is involved in this process, is well-known for its function in blood pressure regulation. In addition to a systemic RAS, the preponderance of target organs have a local RAS. Consequently, RAS hormones and receptors are expressed variably in various types of cancer, the heart, blood vessels, and kidneys. RASi therapy has recently demonstrated promise as a cancer treatment, despite a number of obvious adverse effects, such as hypotension. Consequently, it is essential to perceive how RAS functioned within the tumor microenvironment.
Methods
Single-cell RNA-seq data were acquired from gastric cancer (GC) tumor tissues, and nonnegative matrix factorization (NMF) was used to identify 16 RAS regulators. We evaluated the prognosis and immunological response of TME clusters using GC and Immunotherapy cohorts retrieved from a public repository.
Results
For each cell type (fibroblasts, myeloid cells, T cells, endothelial cells, and mast cells), two or three subclusters were identified based on similar biological processes and marker genes. A connection was discovered between RAS regulatory elements and the clinical and biological aspects of GC, and the pseudotime trajectory of the main TME cell types was also identified. The results of bulk sequencing indicate that these RAS-related TME cell subgroups have a significant immunological response in patients undergoing ICB therapy, especially in CAFs and Tregs, and have a high prognostic value for GC patients. Among the associations uncovered by CellChat's research was the fact that certain TME cell subgroups were associated with RAS. Further investigation revealed that MIF-(CD74 + CXCR4) and MIF-(CD74 + CD44) ligand receptors play a role in RAS-related subgroups' communication with TME cells.
Conclusion
Our research uncovered a previously unknown RAS pathway in the microenvironment of gastric cancer. This route has implications for both the progression of the disease and immunotherapy.
RBP1 promotes the progression of eyelid basal cell carcinoma via regulating OGN expression
Authors: Chen Jiajing , Wang Ting , Yu Luohan , Dai Changming , Liu Longfei , Li Chaopeng 
Published: June 2023

Abstract
Objective
Using RBP1 as the core gene to investigate the effects of RBP1 on the proliferation, migration and invasion of eyelid BCC cell line A431, and to explore its role in a mouse xenograft tumor model. To study in depth the specific molecular mechanisms by which RBP1 promotes the malignant progression of eyelid BCC, RNA-seq as well as bioinformatics analysis was performed to search for downstream genes with the aim of providing new potential targets for the treatment of eyelid BCC.
Methods
A sample of 30 patients with eyelid BCC who underwent surgery in the ophthalmology department of Huai'an First Hospital of Nanjing Medical University was selected. Protein mass spectrometry was used to examine three of the pairs of eyelid BCC cancer tissues and paracancerous tissues, and RBP1 was found to be highly expressed in eyelid BCC. The effect of RBP1 on eyelid BCC was investigated by clinical sample validation, in vitro cytology and mouse xenograft tumor model, and the downstream gene osteoglycin (OGN) was further screened by RNA-seq technology to verify that RBP1 can be involved in eyelid BCC progression by regulating OGN levels.
Results
The results showed that RBP1 was significantly more expressed in eyelid BCC tissues than in paracancerous tissues, and cellular experiments confirmed that RBP1 promoted proliferation, migration and invasion of eyelid BCC cells, and a mouse xenograft tumor model revealed that the gene could promote the growth of cancerous species. Meanwhile, salvage experiments showed that RBP1 accelerated the proliferation, migration, invasion and tumor growth of eyelid BCC cells in nude mice by suppressing OGN protein levels.
Conclusion
RBP1 was significantly upregulated in eyelid BCC. Interfering with RBP1 not only inhibited the proliferation, migration and invasion of A431 cells, but also inhibited the growth of transplanted tumors in nude mice. In addition, knockdown of OGN reversed the inhibitory effects of RBP1 interference on cell proliferation, migration and invasion. In summary, RBP1 can promote tumor progression in eyelid BCC by inhibiting OGN expression, which provides a new potential target for the treatment of eyelid BCC.
Acoustofluidic scanning fluorescence nanoscopy with large field of view
Authors: Huang Tony , Jin Geonsoo , Rich Joseph , Xia Jianping , Upreti Neil , Zhao Chenglong 
Published: June 2023

Abstract
Nanoscale fluorescence imaging with a large-field view is invaluable for many applications such as imaging of subcellular structures, visualizing protein interaction, and high-resolution tissue imaging. Unfortunately, conventional fluorescence microscopy has to make a trade-off between resolution and field of view due to the nature of the optics used to form an image. To overcome this barrier, we have developed an acoustofluidic scanning fluorescence nanoscope that can simultaneously achieve superior resolution, a large field of view, and enhanced fluorescent signal. The acoustofluidic scanning fluorescence nanoscope utilizes the super-resolution capability of microspheres that are controlled by a programable acoustofluidic device for rapid fluorescent enhancement and imaging. The acoustofluidic scanning fluorescence nanoscope can resolve structures that cannot be achieved with a conventional fluorescent microscope with the same objective lens and enhances the fluorescent signal by a factor of ~5 without altering the field of view of the image. The improved resolution with enhanced fluorescent signal and large field of view via the acoustofluidic scanning fluorescence nanoscope provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine, biology, biophysics, and biomedical engineering.
Fluorine labelling for in situ 19F NMR in oriented systems
Authors: Cockburn Kieran T. , Sykes Brian D. 
Published: June 2023

Abstract
The focus of this project is to take advantage of the large NMR chemical shift anisotropy of 19F to determine the orientation of fluorine labeled biomolecules in situ in oriented biological systems such as muscle. The difficulty with a single fluorine atom is that the orientation determined from a chemical shift is not single-valued in the case of a fully anisotropic chemical shift tensor. The utility of a labeling approach with two fluorine labels in a fixed molecular framework where one of the labels has an axially symmetric chemical shift anisotropy such as a CF3 group and the other has a fully asymmetric chemical shift anisotropy such as 5-fluorotryptophan is evaluated. The result is that the orientation of the label can be determined straightforwardly from a single one-dimensional 19F NMR spectrum. The potential applications are widespread and not limited to biological applications.
Foaming mechanism of aluminum-ash-based ceramic pellets under various sintering conditions
Authors: Zhu Xinghan , Yang Yufei , Yang Jinzhong , Huang Qifei , Liu Tao 
Published: June 2023

Abstract
Secondary aluminum dross (SAD) is hazardous waste. Because generation N2 during oxidation reaction of aluminum Nitride (AlN) from SAD, it can be used as forming agent in preparing ceramic pellets. The foaming mechanism of ceramic pellet using SAD was studied. Higher temperature can reduce total amount of pores in sample. When the particle size increased from 0.5 to 2.0 cm, the porosity first decreased and subsequently increased. The porosity was the highest (Si-Al ratio was 1 and the sintering time reached 30 min). The glass network polymerization degree in ceramic pellets decreased at 1250℃ and increased at 1260℃. The polymerization degree increased at a particle size of 1.0 and decreased at 1.5 cm. The change in the degree of polymerization of the glass network affected the liquid-phase region of the melt process. The crystalline products after sintering changed the structural denseness and strength of the pellets considerably.
Prehabilitative Versus Rehabilitative Exercise in Prostate Cancer Patients Undergoing Prostatectomy
Authors: Singh Favil , Newton Robert U , Taaffe Dennis R , Lopez Pedro , Thavaseelan Jeff , Brown Matthew , Ooi Elayne , Nosaka Kazunori , Hayne Dickon , Galvão Daniel A 
Published: June 2023

Abstract
PURPOSE: The study compared the efficacy of commencing supervised exercise in men with prostate cancer before (Prehab) and after (Rehab) prostatectomy on objective and patient-reported outcomes, hospital length of stay (LOS), and urinary incontinence (UI).
METHODS: Forty-one men were randomised to a 6-week Prehab or Rehab exercise programme. Prehabinvolved resistance and aerobic exercise thrice weekly pre-surgery, while Rehabcomprised the same commencing 6-weeks post-surgery. Assessments included strength (1RM), function (chair rise, stair climb, 400-m, 6-m usual, fast, and backwards walk), body composition (DXA), fatigue and quality of life, undertaken at pre-surgery, early post-surgery and late post-surgery phase, with UI (24-hr pad test) assessed at 2, 6, and 12-weeks post-surgery. Intention-to-treat and sensitivity analyses were undertaken.
RESULTS: Of thirty-eight men (48-73 years), 29 completed all assessments with most undergoing robotic-assisted laparoscopic prostatectomy (92.1%). In the pre-surgery phase, Prehab improved muscle strength (leg press: 17.2kg; chest press: 2.9kg; p≤0.001), 400-m, chair rise, 6-m fast and backward walk tests (p<0.001–0.028). Strength and function declines in the early post-surgery phase were maintained late post-surgery. Rehab showed declines of these outcomes after surgery with improvement late post-surgery (leg press: 14.6kg, p<0.001; chest press: 6.8kg, p<0.001; 400-m walk: -12.0sec, p=0.005), resulting in no difference between groups at 12 weeks. There were no significant differences between groups for patient-reported outcomes, hospital LOS or UI.
CONCLUSION: Pre-surgical exercise enhanced strength and function, protecting against post-surgery declines. Although exercise post-surgery is beneficial for recouping strength and function, where possible men undergoing prostatectomy are encouraged to exercise pre-surgery.
Trial registration: ACTRN12617001115325. Registered 31 July 2017.
Alterations in rat brain modular organization during unconsciousness are dependent on communication efficiency and metabolic cost
Authors: Li Siyang , Chen Yali , Ren Peng , Li Zhipeng , Zhang Jun , Liang Xia 
Published: June 2023

Abstract
Spontaneous brain activity exhibits a highly structured modular organization that varies across individuals and reconfigures over time. Although it has been proposed that brain organization is shaped by an economic trade-off between minimizing costs and facilitating efficient information transfer, it remains untested whether modular variability and its changes during unconscious conditions might be constrained by the economy of brain organization. We acquired functional MRI and FDG-PET in rats under three different levels of consciousness induced by propofol administration. We examined alterations in brain modular variability during loss of consciousness from mild sedation to deep anesthesia. We also investigated the relationships between modular variability with glucose metabolism and functional connectivity strength as well as their alterations during unconsciousness. We observed that modular variability increased during loss of consciousness. Critically, across-individual modular variability is oppositely associated with functional connectivity strength and cerebral metabolism, and with deepening dosage of anesthesia, becoming increasingly dependent on basal metabolism over functional connectivity. These results suggested that, propofol-induced unconsciousness may lead to brain modular reorganization, which are putatively shaped by re-negotiations between energetic resources and communication efficiency.
Amelioration of Drought Stress in Wheat by Using Plant Growth Promoting Rhizobacteria (PGPR) and Biogas Slurry
Authors: Raza Muhammad Aown Sammar , Saleem Ahsan , Khan Imran Haider , Tahir Muhammad Ahtisham , Iqbal Rashid , Aslam Muhammad Usman , Alamoudi Soha A. , Ali Baber , Javed Muhammad Ammar , Syaad Khalid M. Al , AlShaqhaa Manal Abdullah 
Published: June 2023

Abstract
Drought has a significant impact on cereal-based staple food production, particularly in developing countries like Pakistan. To ensure a sustainable and reliable food supply, it is essential to develop comprehensive production plans that incorporate various approaches to mitigate the effects of drought. In a study conducted using a randomized complete block design, we investigated the potential of plant growth-promoting rhizobacteria (PGPR) and Biogas Slurry (BGs) either individually or in combination to alleviate drought stress at different stages of wheat growth. The two year field research demonstrated that the application of Azosprillium lipoferumwith Biogas slurry resulted in improved water relations, chlorophyll content, grain quality, yield, and related characteristics in wheat plants compared to the stressed treatments. Particularly, the combined treatment of PGPR and BGs exhibited the most favorable outcomes. Notably, the combined treatment effectively mitigated drought stress by significantly increasing antioxidant levels (17% APX, 29% POD, 34% CAT, and 41% SOD) during the grain-filling stage (GFS) compared to the controls. The combined treatment resulted in a remarkable 40% improvement in the respective controls at the GFS stage. Overall, the combined use of PGPR and BGs was identified as an effective strategy to enhance the resilience of wheat plants to drought, particularly in arid and semi-arid regions.
Effect of fines percentage on Ultrasonic dewatering of Cellulose Nanofibrils
Authors: Ringania Udita , Moon Robert J. , Bhamla M. Saad 
Published: June 2023

Abstract
In this study, we perform a comprehensive examination of ultrasonic dewatering in Cellulose Nanofibril (CNF) suspensions, with particular emphasis on the role of fines content. The production of cellulose nanofibrils (CNF) involves mechanical fibrillation which leads to the presence of different percentages of fines (fibrils under 200 µm) in the final product. Although fines have demonstrated mechanical advantages in composite materials, they also increase water retention by the fibrils, leading to increased dewatering time and energy. We selected two distinct CNF samples with 60% and 90% fines, respectively, and subjected them to ultrasonic drying until 100 wt. % CNF is reached. We found that the 90% fines samples displayed 20% longer drying times, indicating a higher water retention capacity than the 60% fines samples due to increased hydrogen bonding sites. Both fines types exhibit a biphasic pattern in water removal, with the second phase, commencing upon the elimination of half the water, displaying similar rates regardless of the fines content. As dewatering and drying processes often induce agglomeration in CNF, we systematically dewatered both the suspensions until reaching concentrations of 15, 25 and 35 wt.% and then redispersed to 0.01 wt.% CNF. To evaluate the stability of redispersed samples, we monitored their settling behavior and conducted UV-vis transmittance analyses. Results showed that while 60% fines samples could be redispersed in 1 min, the 90% fines samples required up to 5 min to reach a similar level of stability to their original suspensions. Notably, UV-vis transmittance values remained consistent across both the 60% and 90% fines samples and their initial suspensions, indicating a lack of significant agglomeration following redispersion. These findings provide critical insights regarding the impact of CNF fines percentages on dewatering duration and suspension stability during ultrasonic dewatering, contributing to improved processing strategies in industrial cellulose applications.