bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penggunaan Media Sosial untuk Keselamatan Berkendara dalam upaya Membangun Kesadaran Tertib Berlalu Lintas pada Desa Telaga Murni,Kec.Cikarang Barat, Kab.Bekasi
Authors: Rahmawati Roselina , Pangesti Rendy Dwi , Mahbub Jamal , Isnubroto Danang 
Published: January 2023

<p>Media sosial adalah adalah alat komunikasi yang digunakan oleh pengguna dalam proses sosial. Keselamatan menurut Tim Redaksi Kamus Bahasa Indonesia (2008) adalah merujuk pada suatu kondisi yang selamat dalam hal ini kondisi yang terbebas dari bahaya. Pengabdian kepada masyarakat ini mempunyai tujuan untuk menyelenggarakan Sosialisasi Keselamatan Berkendara Sebagai Upaya Membangun Kesadaran Tertib Berlalu Lintas Bagi Masyarakat Melalui Media Sosial. Kegiatan sosialisasi berupa ceramah ini dihadiri oleh 40 peserta. Diikuti oleh 27 orang ibu rumah tangga, 10 orang anak sekolah dan 3 orang balita. Dengan hal ini tampaklah sebanyak 68% kegiatan dihadiri oleh masyarakat usia produktif yang aktif dalam menggunakan internet untuk mengakses media sosial, yaitu ibu rumah tangga. Diharapkan peran aktif ibu rumah tangga yang telah mengikuti sosialisasi agar dapat mengedukasi keluarga selanjutnya untuk dapat menggunakan media sosial dengan baik. Adapun media sosial yang dapat diakses oleh masyarakat dalam hal keselamatan Berkendara dalam upaya Membangun Kesadaran Tertib Berlalu Lintas adalah Media sosial “rsaindonesia”, Media sosial “road_safety”, Media sosial “jddcroadsafety”, dan lain – lain.</p>
Construction of an efficient Claviceps paspali cell factory for lysergic acid production
Authors: Hu Mingzhe , Zhou Yu , Du Siyu , Zhang Xuan , Tang Shen , Yang Yong , Zhang Wei , Chen Shaoxin , Huang Xuenian , Lu Xuefeng 
Published: January 2023

Lysergic acid (LA) is the key precursor of ergot alkaloids, and its derivatives have been used extensively for the treatment of neurological disorders. However, the poor fermentation efficiency limited its industrial application. At the same time, the hardship of genetic manipulation has hindered the metabolic engineering of Claviceps strains to improve the LA titer further. In this study, an efficient genetic manipulation system based on the protoplast-mediated transformation was established in the industrial strain Claviceps paspali. On this basis, the gene lpsB located in the ergot alkaloids biosynthetic gene cluster was deleted to construct the LA-producing cell factory. Plackett-Burman and Box-Behnken designs were used in shaking flasks, achieving an optimal fermentation medium composition. The final titer of LA and iso-lysergic acid (ILA) reached 3.7 g·L−1, which was 4.6 times higher than that in the initial medium. Our work provides an efficient strategy for the biosynthesis of LA and ILA and lays the groundwork for its industrial production.
A novel method for in vivo measurement of dynamic ischiofemoral space based on MRI and motion capture
Authors: Wang Yining , Ma Dong , Feng Zhengkuan , Yu Wanqi , Chen Yanjun , Zhong Shizhen , Ouyang Jun , Qian Lei 
Published: January 2023

Purpose: To use a novel in vivo method to simulate a moving hip model. Then, measure the dynamic bone-to-bone distance, and analyze the ischiofemoral space (IFS) of patients diagnosed with ischiofemoral impingement syndrome (IFI) during dynamic activities.Methods: Nine healthy subjects and 9 patients with IFI were recruited to collect MRI images and motion capture data. The motion trail of the hip during motion capture was matched to a personalized 3D hip model reconstructed from MRI images to get a dynamic bone model. This personalized dynamic in vivo method was then used to simulate the bone motion in dynamic activities. Validation was conducted on a 3D-printed sphere by comparing the calculated data using this novel method with the actual measured moving data using motion capture. Moreover, the novel method was used to analyze the in vivo dynamic IFS between healthy subjects and IFI patients during normal and long stride walking.Results: The validation results show that the root mean square error (RMSE) of slide and rotation was 1.42 mm/1.84° and 1.58 mm/2.19°, respectively. During normal walking, the in vivo dynamic IFS was significantly larger in healthy hips (ranged between 15.09 and 50.24 mm) compared with affected hips (between 10.16 and 39.74 mm) in 40.27%–83.81% of the gait cycle (p = 0.027). During long stride walking, the in vivo dynamic IFS was also significantly larger in healthy hips (ranged between 13.02 and 51.99 mm) than affected hips (between 9.63 and 44.22 mm) in 0%–5.85% of the gait cycle (p = 0.049). Additionally, the IFS of normal walking was significantly smaller than long stride walking during 0%–14.05% and 85.07%–100% of the gait cycle (p = 0.033, 0.033) in healthy hips. However, there was no difference between the two methods of walking among the patients.Conclusions: This study established a novel in vivo method to measure the dynamic bone-to-bone distance and was well validated. This method was used to measure the IFS of patients diagnosed with IFI, and the results showed that the IFS of patients is smaller compared with healthy subjects, whether in normal or long stride walking. Meanwhile, IFI eliminated the difference between normal and long stride walking.
Resting coronary flow drives the daily pattern in coronary flow reserve in patients with chest pain without obstructive epicardial stenosis
Authors: Thosar Saurabh S. , Taqui Sahar , Davidson Brian , Belcik Todd , Hodovan James , Rice Sean P. M. , Lindner Jonathan R. 
Published: January 2023

ObjectivesIschemia with no obstructive coronary artery disease (INOCA) is a risk factor for major adverse cardiovascular events and is characterized by abnormal coronary microvascular tone. In patients with INOCA, adverse cardiovascular events most commonly occur in the morning compared to other times of the day and night.Materials and methodsWe tested whether coronary microvascular function varies diurnally with attenuation in the morning in patients with symptomatic coronary artery disease without significant (&gt;50%) epicardial stenosis. We evaluated data from 17 patients studied in the AM (700–1159 h) and 11 patients in the PM (1200–1800 h). Coronary microvascular function was measured using perfusion contrast imaging at rest and after infusion of intravenous regadenoson. We calculated microvascular flow reserve as the ratio of hyperemic to resting flow. Along with independent sample t-tests, we performed bootstrapping procedures to test mean differences between AM and PM groups, using the bias-corrected and accelerated method with 5,000 bootstrapped samples.Results and conclusionThe AM and PM groups were matched for demographic and existing risk factors. Coronary microvascular flow reserve was ∼33% higher in the AM compared to the PM (P = 0.025, BCa 95% CI [0.25, 1.64]; Hedge’s g = 0.89, 95% CI [0.11, 1.66]) as a result of significantly lower resting flow (∼50%) in the AM compared to the PM (P = 0.03, MDiff = −56.65, BCa 95% CI [−118.59, −2.12]; Hedge’s g = −0.86, 95% CI [−1.60, −0.06]). Our observations are of clinical value and can influence diagnosis and treatment in the clinic based on the time of day of measurements.
Liuzijue training improves hypertension and modulates gut microbiota profile
Authors: Wu Sha , Zheng Caiping , Liu Nannan , Deng Tingting , Wang Jie , Qi Luming , Xia Lina 
Published: January 2023

BackgroundLiuzijue training (LZJ) is a traditional exercise integrating breathing meditation and physical exercise, which could prevent and improve hypertension symptoms.PurposeWe aimed to evaluate the therapeutic effect of LZJ on hypertensive patients from the perspectives of blood pressure (BP), vascular endothelial function, immune homeostasis, and gut microbiota.MethodsWe conducted a randomized, controlled, single-blind experiment to assess the effect of 12 weeks LZJ in hypertensive patients. We measured the blood pressure level, vascular endothelial function, serum inflammatory factor concentration, and fecal microbial composition of hypertension patients.ResultsCompared with aerobic training, LZJ has a more significant effect on serum inflammatory factors (IL-6 and IL-10) and gut microbiota. PCoA analysis showed that LZJ tended to transform the gut microbiota structure of hypertensive subjects into that of healthy people. This process involves significant changes in Bacteroides, Clostridium_sensu_stricto_1, Escherichia-Shigella, Haemophilus, Megamonas, and Parabacteroides. In particular, Bacteroides and Escherichia-Shigella, these bacteria were closely related to the improvement of BP in hypertensive patients.ConclusionIn conclusion, our results confirm that LZJ could be used as an adjuvant treatment for hypertensive patients, which could effectively reduce BP, improve the immune homeostasis and gut microbiota structure in patients, and provide a theoretical reference for the use of LZJ in the clinic.Clinical trial registrationhttp://www.chictr.org.cn/listbycreater.aspx, identifier: ChiCTR2200066269.
Disrupting arrhythmia in a professional male wrestler athlete after rapid weight loss and high-intensity training—Case report
Authors: Milovančev Aleksandra , Miljković Tatjana , Ilić Aleksandra , Stojšić Milosavljević Anastazija , Petrović Milovan , Roklicer Roberto , Lakičević Nemanja , Trivić Tatjana , Drid Patrik 
Published: January 2023

IntroductionPhysiological heart adaptations may lead to increased susceptibility to arrhythmia in athletes. Furthermore, vigorous training and method like rapid weight loss (RWL) in combat sports could pose additional risks. This case represents how routine cardiovascular screening during high-risk methods like RWL and high-intensity training (HIT) reveal abrupt ventricular arrhythmias in a young athlete.Case reportWe report a case of a 20-year-old male wrestler athlete who developed disrupting arrhythmia during RWL and HIT. The study included: a medical exam, 12 lead electrocardiograms (ECG), transthoracic echocardiogram (ECHO), and 24 h of continuous ECG monitoring in baseline, phase one (P1), (in which the athlete had to simulate RWL through vigorous training and dietary intervention and HIT) and phase two (P2), (with the same HIT protocol performed without the RWL procedure). Baseline laboratory analyses were without abnormalities, ECG showed sinus rhythm with one premature atrial contraction (PAC) and ECHO showed signs of concentric remodeling with preserved systolic, diastolic function, and global longitudinal strain. After P1 RWL simulation, he lost 5.15% of body weight in 3 days, which resulted in lower blood glucose levels, higher urea, creatinine, creatine kinase (CK), CK-MB levels, and slightly increased levels of NT pro-BNP, ECG revealed sinus rhythm with one ventricular premature beat (VPB), 24-h continuous electrocardiogram (ECG) revealed frequent ventricular premature beats (PVB) 2,150/ 24 h, with two couplets, and 8 PAC. After an advised 4-week period of de-training continuous 24 h, ECG monitoring was improved with only occasional PVB. The 24 h continuous ECG monitoring was repeated after HIT and revealed even more frequent PVB, 5% of all beats for 24 h, 4,205 in total, and almost all VPB were in bigeminy and trigeminy. The athlete was advised against RWL and extremely vigorous exercise and for regular, frequent checkups with occasional ECG monitoring during and after exercise.ConclusionThe short and long-term implication of abrupt ventricular arrhythmias provoked by intensive training and methods like RWL is unknown. We postulate that cardiovascular screening is necessitated, especially during high-risk methods like RWL and HIT, in helping us prevent adverse outcomes and come to individual-based clinical making decisions for each athlete.
Co-occurrence of mutations in NF1 and other susceptibility genes in pheochromocytoma and paraganglioma
Authors: Mellid Sara , Gil Eduardo , Letón Rocío , Caleiras Eduardo , Honrado Emiliano , Richter Susan , Palacios Nuria , Lahera Marcos , Galofré Juan C. , López-Fernández Adriá , Calatayud Maria , Herrera-Martínez Aura D. , Galvez María A. , Matias-Guiu Xavier , Balbín Milagros , Korpershoek Esther , Lim Eugénie S. , Maletta Francesca , Lider Sofia , Fliedner Stephanie M. J. , Bechmann Nicole , Eisenhofer Graeme , Canu Letizia , Rapizzi Elena , Bancos Irina , Robledo Mercedes , Cascón Alberto 
Published: January 2023

IntroductionThe percentage of patients diagnosed with pheochromocytoma and paraganglioma (altogether PPGL) carrying known germline mutations in one of the over fifteen susceptibility genes identified to date has dramatically increased during the last two decades, accounting for up to 35-40% of PPGL patients. Moreover, the application of NGS to the diagnosis of PPGL detects unexpected co-occurrences of pathogenic allelic variants in different susceptibility genes.MethodsHerein we uncover several cases with dual mutations in NF1 and other PPGL genes by targeted sequencing. We studied the molecular characteristics of the tumours with co-occurrent mutations, using omic tools to gain insight into the role of these events in tumour development.ResultsAmongst 23 patients carrying germline NF1 mutations, targeted sequencing revealed additional pathogenic germline variants in DLST (n=1) and MDH2 (n=2), and two somatic mutations in H3-3A and PRKAR1A. Three additional patients, with somatic mutations in NF1 were found carrying germline pathogenic mutations in SDHB or DLST, and a somatic truncating mutation in ATRX. Two of the cases with dual germline mutations showed multiple pheochromocytomas or extra-adrenal paragangliomas - an extremely rare clinical finding in NF1 patients. Transcriptional and methylation profiling and metabolite assessment showed an “intermediate signature” to suggest that both variants had a pathological role in tumour development.DiscussionIn conclusion, mutations affecting genes involved in different pathways (pseudohypoxic and receptor tyrosine kinase signalling) co-occurring in the same patient could provide a selective advantage for the development of PPGL, and explain the variable expressivity and incomplete penetrance observed in some patients.
Safety of intraocular anti-VEGF antibody treatment under in vitro HTLV-1 infection
Authors: Zong Yuan , Kamoi Koju , Kurozumi-Karube Hisako , Zhang Jing , Yang Mingming , Ohno-Matsui Kyoko 
Published: January 2023

IntroductionHTLV-1 (human T-cell lymphotropic virus type 1) is a retrovirus that infects approximately 20 million people worldwide. Many diseases are caused by this virus, including HTLV-1–associated myelopathy, adult T-cell leukemia, and HTLV-1 uveitis. Intraocular anti–vascular endothelial growth factor (VEGF) antibody injection has been widely used in ophthalmology, and it is reportedly effective against age-related macular degeneration, complications of diabetic retinopathy, and retinal vein occlusions. HTLV-1 mimics VEGF165, the predominant isoform of VEGF, to recruit neuropilin-1 and heparan sulfate proteoglycans. VEGF165 is also a selective competitor of HTLV-1 entry. Here, we investigated the effects of an anti-VEGF antibody on ocular status under conditions of HTLV-1 infection in vitro.MethodsWe used MT2 and TL-Om1 cells as HTLV-1–infected cells and Jurkat cells as controls. Primary human retinal pigment epithelial cells (HRPEpiCs) and ARPE19 HRPEpiCs were used as ocular cells; MT2/TL-Om1/Jurkat cells and HRPEpiCs/ARPE19 cells were co-cultured to simulate the intraocular environment of HTLV-1–infected patients. Aflibercept was administered as an anti-VEGF antibody. To avoid possible T-cell adhesion, we lethally irradiated MT2/TL-Om1/Jurkat cells prior to the experiments.ResultsAnti-VEGF antibody treatment had no effect on activated NF-κB production, inflammatory cytokines, chemokines, HTLV-1 proviral load (PVL), or cell counts in the retinal pigment epithelium (RPE) under MT2 co-culture conditions. Under TL-Om1 co-culture conditions, anti-VEGF antibody treatment did not affect the production of activated NF-κB, chemokines, PVL, or cell counts, but production of the inflammatory cytokine IL-6 was increased. In addition, anti-VEGF treatment did not affect PVL in HTLV-1–infected T cells.ConclusionThis preliminary in vitro assessment indicates that intraocular anti-VEGF antibody treatment for HTLV-1 infection does not exacerbate HTLV-1–related inflammation and thus may be safe for use.
The regulation and function of acetylated high-mobility group box 1 during implantation and decidualization
Authors: Li Yue , Chen Si-Ting , He Yu-Ying , Li Bo , Yang Chen , Yang Zhen-Shan , Yang Zeng-Ming 
Published: January 2023

IntroductionHigh-mobility group box 1 (HMGB1) is a non-histone nuclear protein and can be extracellularly secreted to induce sterile inflammation. Although uterine deletion of HMGB1 causes implantation and decidualization defects, how secreted HMGB1 is involved in mouse early pregnancy is still unknown.MethodsMouse models, mouse primary endometrial cells and human endometrial cell lines were used in this study. Both immunofluorescence and Western blot were performed to show the localization and relative level of HMGB1 and acetylated HMGB1, respectively. Relative mRNA levels were analyzed by real time RT-PCR.ResultsThe secreted HMGB1 was detected in uterine lumen fluid in mouse periimplantation uterus. There is an obvious difference for secreted HMGB1 levels in uterine fluid between day 4 of pregnancy and day 4 of pseudopregnancy, suggesting the involvement of blastocysts during HMGB1 secretion. Trypsin is clearly detected in mouse blastocyst cavity and in the supernatant of cultured blastocysts. Trypsin significantly stimulates HB-EGF production through activating PAR2 and ADAM17. Uterine injection of PAR2 inhibitor into day 4 pregnant mice significantly reduces the number of implantation sites. HB-EGF released from luminal epithelium can induce mouse in vitro decidualization. The conditioned medium collected from trypsin-treated luminal epithelium is able to induce in vitro decidualization, which is suppressed by EGFR inhibitor. Intrauterine injection of glycyrrhizin (HMGB1 inhibitor) can significantly inhibit mouse embryo implantation. We also showed that exogenous HMGB1 released from human epithelial cells are able to induce human in vitro decidualization.ConclusionTrypsin can induce decidualization of stromal cells via PAR2-HMGB1-ADAM17-HB-EGF from luminal epithelium.
Altered early immune response after fracture and traumatic brain injury
Authors: Haffner-Luntzer Melanie , Weber Birte , Morioka Kazuhito , Lackner Ina , Fischer Verena , Bahney Chelsea , Ignatius Anita , Kalbitz Miriam , Marcucio Ralph , Miclau Theodore 
Published: January 2023

IntroductionClinical and preclinical data suggest accelerated bone fracture healing in subjects with an additional traumatic brain injury (TBI). Mechanistically, altered metabolism and neuro-endocrine regulations have been shown to influence bone formation after combined fracture and TBI, thereby increasing the bone content in the fracture callus. However, the early inflammatory response towards fracture and TBI has not been investigated in detail so far. This is of great importance, since the early inflammatory phase of fracture healing is known to be essential for the initiation of downstream regenerative processes for adequate fracture repair.MethodsTherefore, we analyzed systemic and local inflammatory mediators and immune cells in mice which were exposed to fracture only or fracture + TBI 6h and 24h after injury.ResultsWe found a dysregulated systemic immune response and significantly fewer neutrophils and mast cells locally in the fracture hematoma. Further, local CXCL10 expression was significantly decreased in the animals with combined trauma, which correlated significantly with the reduced mast cell numbers.DiscussionSince mast cells and mast cell-derived CXCL10 have been shown to increase osteoclastogenesis, the reduced mast cell numbers might contribute to higher bone content in the fracture callus of fracture + TBI mice due to decreased callus remodeling.
Safety of SARS-CoV2 vaccination and COVID-19 short-term outcome in pediatric acquired demyelinating disorders of central nervous system: A single center experience
Authors: Monte Gabriele , Papetti Laura , Ferilli Michela Ada Noris , Ursitti Fabiana , Moavero Romina , Sforza Giorgia , Panella Elena , Tarantino Samuela , Checchi Martina Proietti , Vigevano Federico , Palma Paolo , Valeriani Massimiliano 
Published: January 2023

IntroductionConcern of a correlation between disease relapse in patients with acquired demyelinating disorders of central nervous system (CNS) and SARS-CoV2 vaccines has been raised. In this single center study, we retrospectively evaluated safety of SARS-CoV2 vaccination and COVID-19 short-term outcome in pediatric acquired demyelinating disorders of CNS.Materials and methodsPatients with multiple sclerosis (MS), myelin oligodendrocyte glycoprotein antibody associated disease (MOGAD) and neuromyelitis optica spectrum disorder (NMOSD) with disease onset before 18 years of age were included. Demographic and clinical data, and information regarding previous SARS-CoV-2 infection and vaccination were collected.ResultsWe included nine patients with MOGAD. Six patients received SARS-CoV2 vaccination and complained pain at injection site while only one had fever and fatigue. Median follow-up was 28 weeks (range 20-48). Seven patients had COVID-19 occurring with mild flu-like symptoms and median follow-up was 28 weeks (range 24-34). Nobody had disease relapse. Five patients with NMOSD were included. All patients received SARS-CoV2 vaccination (BNT162b2-Pfizer-BioNTech). The median follow-up was 20 weeks (range 14-24) and only two patients complained pain at injection site, fever and fatigue. Three patients had also COVID-19 with mild flu-like symptoms, despite two of them being under immunosuppressive treatment. Lastly, forty-three patients with MS were included. 35 out of 43 received SARS-CoV2 vaccination with a median follow-up of 24 weeks (range 8-36). Fourteen patients had no side effects, while 21 complained mild side effects (mainly pain at injection site) and one experienced a disease relapse with complete recovery after steroid therapy. At vaccination, all but one were under treatment. Sixteen patients had COVID-19 occurring with mild symptoms.DiscussionCOVID-19 outcome was good although many patients were under immunosuppressive treatment. Vaccine-related side effects were frequent but were mild and self-limited. Only one MS patient had a post-vaccination relapse with complete recovery after steroid therapy. In conclusion, our data support the safety of SARS-CoV-2 vaccines in pediatric MS, MOGAD and NMOSD.