bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measuring the Roller Skew Angle in the Loading Zone of a Cylindrical Roller Bearing With Strain Gauges for Long-Term Monitoring
Authors: Zhao Zhixiang , Wang Xi , Hou Yu 
Published: August 2023

Abstract
Roller skew in roller bearings can cause heat generation in roller–race and roller–rib contacts, thus reducing bearing life. It is significant to obtain the roller skew angle in situ for guiding the bearing design and adjusting the operating conditions. In this study, a method for measuring the roller skew angle in the loading zone of a cylindrical roller bearing with strain gauges is presented. The measurement principle is that the roller skew angle is related to the movement of the contact line between the roller and raceway. The strain gauge array on the outer surface of the outer ring shows temporally separated responses when roller skew occurs. An experimental system is developed to validate the effectiveness of the proposed method for cylindrical roller bearing measurements. A laser measurement system is incorporated into the experimental system to detect the horizontal displacement of the reflected spot using a high-speed camera, which is then converted into the roller skew angle. The calculated roller skew angle from the time shift of the strain response agrees well with the value of a specially modified roller independently measured with the laser detection system. Compared with other measurement methods, the proposed method provides a potential way to achieve the nondestructive measurement of the roller skew angle in actual service for long-term purposes.
Material Extrusion on an Ultrasonic Air Bed for 3D Printing
Authors: Keller Samuel , Stein Matthew , Ilic Ognjen 
Published: August 2023

Abstract
Additive manufacturing, such as 3D printing, offers unparalleled opportunities for rapid prototyping of objects, but typically requires simultaneous building of solid supports to minimize deformation and ensure contact with the printing surface. Here, we theoretically and experimentally investigate the concept of material extrusion on an “air bed”—an engineered ultrasonic acoustic field that stabilizes and supports the soft material by contactless radiation pressure force. We study the dynamics of polylactic acid filament—a commonly used material in 3D printing—as it interacts with the acoustic potential during extrusion. We develop a numerical radiation pressure model to determine optimal configurations of ultrasonic transducers to generate acoustic fields and conditions for linear printing. We build a concept prototype that integrates an acoustic levitation array with a 3D printer and use this device to demonstrate linear extrusion on an acoustic air bed. Our results indicate that controlled interactions between acoustic fields and soft materials could offer alternative support mechanisms in additive manufacturing with potential benefits such as less material waste, fewer surface defects, and reduced material processing time.
Blood Lactate Responses of Male and Female Players Across an International Rugby Sevens Tournament
Authors: James Carl , Rees James , Chong Henry , Taylor Lee , Beaven Christopher M. , Henderson Mitch , Baker Julien S. 
Published: August 2023

Purpose: This study investigated within- and between-matches blood lactate (La−) responses across an international Rugby Sevens tournament (5 matches over 2 d) in male and female players. Methods: Earlobe blood samples were taken from 25 professional players around matches: before warm-up (PRE), immediately upon finishing match participation (POST), and 30 minutes postmatch (30 min). Results: POST [La−] (mean [SD], range) for males was 10.3 (3.2; 2.9–20.2) mmol·L−1 and for females was 9.1 (2.3; 3.4–14.6) mmol·L−1. Linear mixed-effects models revealed a decrease in POST [La−] after match 5, compared to match 1. Increased PRE [La−] was found before match 2 (+0.8 [0.6–1.1] mmol·L−1), match 3 (+0.8 [0.5–1.1] mmol·L−1), and match 5 (+0.6 [0.4–0.9] mmol·L−1) compared to match 1 (all P < .001). The [La−] remained elevated at 30 min, compared to PRE (+1.7 [1.4–2.0] mmol·L−1, P < .001), with ∼20% of values persisting >4 mmol·L−1. Higher POST was observed in males compared to females (+1.6 [0.1–3.2] mmol·L−1, P = .042); however, no differences between sexes were found across 30 min or PRE [La−]. No [La−] differences between positions (backs and forwards) were identified. Conclusions: Lactate concentrations above 10 mmol·L−1 are required to effectively simulate the anaerobic demands of international Rugby Sevens matches. Practitioners are advised to individualize anaerobic training prescription due to the substantial variability observed within positional groups. Additionally, improving athletes’ metabolic recovery capacity through training, nutrition, and recovery interventions may enhance physical preparation for subsequent matches within a day, where incomplete lactate clearance was observed.
Apolipoprotein E genotype affects innate susceptibility to
Listeria monocytogenes
infection in aged male mice
Authors: Cho Jooyoung , Alexander Katie L. , Ferrell Jessica L. , Johnson Lance A. , Estus Steven , D’Orazio Sarah E. F. 
Published: August 2023

ABSTRACT

Apolipoprotein E (ApoE) is a lipid transport protein that is hypothesized to suppress proinflammatory cytokine production, particularly after stimulation with Toll-like receptor (TLR) ligands such as lipopolysaccharide (LPS). Studies using transgenic ApoE human replacement mice (APOE) expressing one of three different allelic variants suggest that there is a hierarchy in terms of responsiveness to proinflammatory stimuli such as APOE4/E4 > APOE3/E3 > APOE2/E2. In this study, we test the hypothesis that
APOE
genotype can also predict susceptibility to infection with the facultative intracellular gram-positive bacterium
Listeria monocytogenes
. We found that bone-marrow-derived macrophages isolated from aged APOE4/E4 mice expressed elevated levels of nitric oxide synthase 2 and were highly resistant to
in vitro
infection with
L. monocytogenes
compared to APOE3/E3 and APOE2/E2 mice. However, we did not find statistically significant differences in cytokine or chemokine output from either macrophages or whole splenocytes isolated from APOE2/E2, APOE3/E3, or APOE4/E4 mice following
L. monocytogenes
infection.
In vivo
, overall susceptibility to foodborne listeriosis also did not differ by
APOE
genotype in either young (2 mo old) or aged (15 mo old) C57BL/6 mice. However, we observed a sex-dependent susceptibility to infection in aged APOE2/E2 male mice and a sex-dependent resistance to infection in aged APOE4/E4 male mice that was not present in female mice. Thus, these results suggest that
APOE
genotype does not play an important role in innate resistance to infection with
L. monocytogenes
but may be linked to sex-dependent changes that occur during immune senescence.
Coding-complete genome sequence of an isolate of papaya virus E in tomato
Authors: Abrahamian Peter , Mollov Dimitre , Hammond Rosemarie W. , Rivera Yazmin 
Published: August 2023

ABSTRACT
An isolate of papaya virus E was identified in tomato fruits from Mexico. The coding-complete genome sequence was determined using high-throughput sequencing. The coding-complete genome is 13,412 nucleotides and contains 8 open reading frames.
Rapid, efficient auxin-inducible protein degradation in
Candida
pathogens
Authors: Milholland Kedric L. , Gregor Justin B. , Hoda Smriti , Píriz-Antúnez Soledad , Dueñas-Santero Encarnación , Vu Bao Gia , Patel Krishna P. , Moye-Rowley W. Scott , Vázquez de Aldana Carlos R. , Correa-Bordes Jaime , Briggs Scott D. , Hall Mark C. 
Published: August 2023

ABSTRACT

A variety of inducible protein degradation (IPD) systems have been developed as powerful tools for protein functional characterization. IPD systems provide a convenient mechanism for rapid inactivation of almost any target protein of interest. Auxin-inducible degradation (AID) is one of the most common IPD systems and has been established in diverse eukaryotic research model organisms. Thus far, IPD tools have not been developed for use in pathogenic fungal species. Here, we demonstrate that the original AID and the second generation, AID2, systems work efficiently and rapidly in the human pathogenic yeasts,
Candida albicans
and
Candida glabrata
. We developed a collection of plasmids that support AID system use in laboratory strains of these pathogens. These systems can induce >95% degradation of target proteins within minutes. In the case of AID2, maximal degradation was achieved at low nanomolar concentrations of the synthetic auxin analog 5-adamantyl-indole-3-acetic acid. Auxin-induced target degradation successfully phenocopied gene deletions in both species. The system should be readily adaptable to other fungal species and to clinical pathogen strains. Our results define the AID system as a powerful and convenient functional genomics tool for protein characterization in fungal pathogens.

IMPORTANCE

Life-threatening fungal infections are an escalating human health problem, complicated by limited treatment options and the evolution of drug resistant pathogen strains. Identification of new targets for therapeutics to combat invasive fungal infections, including those caused by
Candida
species, is an urgent need. In this report, we establish and validate an inducible protein degradation methodology in
Candida albicans
and
Candida glabrata
that provides a new tool for protein functional characterization in these, and likely other, fungal pathogen species. We expect this tool will ultimately be useful for the identification and characterization of promising drug targets and factors involved in virulence and drug resistance.
Engineered phage with cell-penetrating peptides for intracellular bacterial infections
Authors: Zhao Min , Tan Xin , Liu Zi-qiang , Dou Lei , Liu Dong , Pan Yong-jun , Ma Ying-fei , Yu Jia-lin 
Published: August 2023

ABSTRACT

Salmonella
infection poses a critical challenge to global public health, and the situation is exacerbated by the increasing prevalence of antibiotic resistance. Bacteriophages (phages) are increasingly being used as antimicrobial agents due to their ability to kill specific bacteria. However, the low cellular uptake of phages has limited their use in treating intracellular bacterial infections. Here, we present a study using engineered phages with cell-penetrating peptides (CPPs) for enhancing the internalization efficiency of phages to inhibit bacterial intracellular infections. Through bioinformatic analysis, we identified a phage-encoded protein harboring an immunoglobulin-like (Ig-like) domain as the potential target for phage display. Using a CRISPR-Cas9-based method, we successfully displayed short peptides on GP94, an Ig-like domain-containing protein, of
Salmonella
phage selz. We improved phage intracellular uptake in multiple cell types by fusion of various CPPs to GP94. Notably, the phage selz
HA-TAT
showed promising results in enhancing the intracellular inhibition of
Salmonella
in different cells. Our research provides a straightforward strategy for displaying CPPs on non-model phages, offering a promising novel and effective therapeutic approach for treating intracellular and drug-resistant bacteria.

IMPORTANCE

Salmonella
infection is a significant threat to global public health, and the increasing prevalence of antibiotic resistance exacerbates the situation. Therefore, finding new and effective ways to combat this pathogen is essential. Phages are natural predators of bacteria and can be used as an alternative to antibiotics to kill specific bacteria, including drug-resistant strains. One significant limitation of using phages as antimicrobial agents is their low cellular uptake, which limits their effectiveness against intracellular bacterial infections. Therefore, finding ways to enhance phage uptake is crucial. Our study provides a straightforward strategy for displaying cell-penetrating peptides on non-model phages, offering a promising novel and effective therapeutic approach for treating intracellular and drug-resistant bacteria. This approach has the potential to address the global challenge of antibiotic resistance and improve public health outcomes.
La-related protein 4 is enriched in vaccinia virus factories and is required for efficient viral replication in primary human fibroblasts
Authors: Dhungel Pragyesh , Brahim Belhaouari Djamal , Yang Zhilong 
Published: August 2023

ABSTRACT
In addition to the 3′-poly(A) tail, vaccinia virus mRNAs synthesized after viral DNA replication (post-replicative mRNAs) possess a 5′-poly(A) leader that confers a translational advantage in virally infected cells. These mRNAs are synthesized in viral factories, the cytoplasmic compartment where vaccinia virus DNA replication, mRNA synthesis, and translation occur. However, a previous study indicates that the poly(A)-binding protein (PABPC1)—which has a well-established role in RNA stability and translation—is absent in the viral factories. This prompts the question of whether other poly(A)-binding proteins engage vaccinia virus post-replicative mRNA in viral factories. Here, in this study, we found that La-related protein 4 (LARP4), a poly(A) binding protein, was enriched in viral factories in multiple types of cells during vaccinia virus infection. Further studies showed that LARP4 enrichment in the viral factories required viral post-replicative gene expression and functional decapping enzymes encoded by vaccinia virus. We further showed that knockdown of LARP4 expression in human foreskin fibroblasts (HFFs) reduced vaccinia virus DNA replication, post-replicative protein levels, and viral production. Interestingly, the knockdown of LARP4 expression also reduced protein levels from transfected mRNA containing a 5′-poly(A) leader in vaccinia virus-infected and uninfected HFFs. Taken together, our results identified a poly(A)-binding protein, LARP4, being enriched in the vaccinia virus viral factories and facilitating viral replication in HFFs.

IMPORTANCE
Vaccinia virus, the prototype poxvirus, encodes over 200 open reading frames (ORFs). Over 90 of vaccinia virus ORFs are transcribed post-viral DNA replication. All these mRNAs contain a 5′-poly(A) leader, as well as a 3′-poly(A) tail. They are synthesized in viral factories, where vaccinia virus DNA replication, mRNA synthesis, and translation occur. However, surprisingly, the poly(A) binding protein, PABPC1, that is important for mRNA metabolism and translation is not present in the viral factories, suggesting other poly(A) binding protein(s) may be present in viral factories. Here, we found another poly(A)-binding protein, La-related protein 4 (LARP4), enriched in viral factories during vaccinia virus infection. We also showed that LARP4 enrichment in the viral factories depends on viral post-replicative gene expression and functional viral decapping enzymes. The knockdown of LARP4 expression in human foreskin fibroblasts reduced vaccinia virus DNA replication, post-replicative gene expression, and viral production.
In vitro
and intracellular activities of novel thiopeptide derivatives against macrolide-susceptible and macrolide-resistant
Mycobacterium avium
complex
Authors: Park Jiyun , Kim Lee-Han , Lee Ju Mi , Choi Sangwon , Son Young-Jin , Hwang Hee-Jong , Shin Sung Jae 
Published: August 2023

ABSTRACT

Unsatisfactory outcomes following long-term multidrug treatment in patients with
Mycobacterium avium
complex (MAC) pulmonary disease have urged us to develop novel antibiotics. Thiopeptides, a class of peptide antibiotics derived from natural products, have potential as drug candidates that target bacterial ribosomes, but drug development has been hampered due to their extremely poor solubility. Here, we evaluated three new compounds (AJ-037, AJ-039, and AJ-206) derived from the thiopeptide micrococcin P2 with enhanced aqueous solubility; the derivatives were generated based on structure-activity relationship analysis. We conducted
in vitro
drug susceptibility and intracellular antimycobacterial activity testing of the three thiopeptide derivatives against various MAC strains, including macrolide-resistant MAC clinical isolates. These compounds showed low MICs against MAC, similar to that of clarithromycin (CLR). In particular, two compounds, AJ-037 and AJ-206, had intracellular antimycobacterial activities, along with synergistic effects with CLR, and inhibited the growth of MAC inside macrophages. Moreover, these two compounds showed
in vitro
and intracellular anti-MAC activities against macrolide-resistant MAC strains without showing cross-resistance with CLR. Taken together, the results of the current study suggest that AJ-037 and AJ-206 can be promising anti-MAC agents for the treatment of MAC infection, including for macrolide-resistant MAC strains.

IMPORTANCE

Novel antibiotics for the treatment of MAC infection are urgently needed because the treatment outcomes using the standard regimen for
Mycobacterium avium
complex (MAC) pulmonary disease remain unsatisfactory. Here, we evaluated three novel thiopeptide derivatives (AJ-037, AJ-039, and AJ-206) derived from the thiopeptide micrococcin P2, and they were confirmed to be effective against macrolide-susceptible and macrolide-resistant MAC. Our thiopeptide derivatives have enhanced aqueous solubility through structural modifications of poorly soluble thiopeptides. The thiopeptide derivatives showed minimal inhibitory concentrations against MAC that were comparable to clarithromycin (CLR). Notably, two compounds, AJ-037 and AJ-206, exhibited intracellular antimycobacterial activities and acted synergistically with CLR to hinder the growth of MAC within macrophages. Additionally, these compounds demonstrated
in vitro
and intracellular anti-MAC activities against macrolide-resistant MAC strains without showing any cross-resistance with CLR. We believe that AJ-037 and AJ-206 can be promising anti-MAC agents for the treatment of MAC infections, including macrolide-resistant MAC strains.
Detrital zircon and apatite U-Pb provenance and drainage evolution of the Newark Basin during progressive rifting and continental breakup along the Eastern North American Margin, USA
Authors: Foster-Baril Zachary S. , Stockli Daniel F. 
Published: August 2023

AbstractMesozoic rift basins of the Eastern North American Margin (ENAM) span from Florida in the United States to the Grand Banks of Canada and formed during progressive extension prior to continental breakup and the opening of the north-central Atlantic. The syn-rift strata from all the individual basins, lumped along the entire margin into the Newark Supergroup, are dominated by fluvial conglomerate and sandstone, lacustrine siltstone, mudstone, and abundant alluvial conglomerate and sandstone lithofacies. Deposition of these syn-rift sedimentary rocks was accommodated in a series of half grabens and subsidiary full grabens situated within the Permo-Carboniferous Appalachian orogen. The Mesozoic ENAM is commonly depicted as a magma-rich continental rift margin, with magmatism (Central Atlantic magmatic province [CAMP]) driving continental breakup. However, the southern portion of the ENAM shows evidence of magmatic breakup (e.g., seaward-dipping reflectors), and rifting and crustal thinning appeared to start ~30 m.y. prior to CAMP emplacement in the Jurassic. This study provides extensive new detrital zircon and apatite U-Pb provenance data to determine the provenance and reconstruct the paleodrainages of the Newark Basin during progressive rifting and magmatic breakup and the implications for the overall rift configuration and asymmetry during progressive rifting along the ENAM rift margin. Detailed new detrital zircon (N = 21; n = 3093) and apatite (N = 4; n = 559) U-Pb results from sandstone outcrop and core samples from the Newark Basin indicate a distinct provenance shift, with relatively older Carnian syn-rift strata predominately sourced from the hanging wall of the basin bounding fault in the east while relatively younger Norian strata were regionally sourced from both the hanging wall and footwall. The syn-rift strata at the Triassic-Jurassic boundary were sourced from the hanging wall before a transition to local footwall terranes. These results suggest two major provenance changes during progressive rifting—the first occurring during Carnian crustal necking and rift flank uplift as predicted by recent numerical models and the second occurring at the onset of the Jurassic due to regional and local thermal uplift during CAMP magmatism as seen along other magma-rich margins, such as the North Atlantic and the southern portion of the South Atlantic margin.
Pathophysiology, diagnosis, and management of neuroinflammation in covid-19
Authors: Brown Rachel L , Benjamin Laura , Lunn Michael P , Bharucha Tehmina , Zandi Michael S , Hoskote Chandrashekar , McNamara Patricia , Manji Hadi 
Published: August 2023

Abstract
Although neurological complications of SARS-CoV-2 infection are relatively rare, their potential long term morbidity and mortality have a significant impact, given the large numbers of infected patients. Covid-19 is now in the differential diagnosis of a number of common neurological syndromes including encephalopathy, encephalitis, acute demyelinating encephalomyelitis, stroke, and Guillain-Barré syndrome. Physicians should be aware of the pathophysiology underlying these presentations to diagnose and treat patients rapidly and appropriately. Although good evidence has been found for neurovirulence, the neuroinvasive and neurotropic potential of SARS-CoV-2 is limited. The pathophysiology of most complications is immune mediated and vascular, or both. A significant proportion of patients have developed long covid, which can include neuropsychiatric presentations. The mechanisms of long covid remain unclear. The longer term consequences of infection with covid-19 on the brain, particularly in terms of neurodegeneration, will only become apparent with time and long term follow-up.
Digitisation of patient preferences in palliative care: mobile app prototype
Authors: Ferreira João , Ferreira Marta , Fernandes Carla Sílvia , Castro João , Campos Maria Joana 
Published: August 2023

BackgroundEngaging in advance care planning can be emotionally challenging, but gamification and technology are suggested as a potential solution.ObjectivePresent the development stages of a mobile app prototype to improve quality of life for patients in palliative care.DesignThe study started with a comprehensive literature review to establish a foundation. Subsequently, interviews were conducted to validate the proposed features of the mobile application. Following the development phase, usability tests were conducted to evaluate the overall usability of the mobile application. Furthermore, an oral questionnaire was administered to understand user satisfaction about the implemented features.ResultsA three-phase testing approach was employed based on the chosen user-centred design methodology to obtain the results. Three iterations were conducted, with improvements being made based on feedback and tested in subsequent phases. Despite the added complexity arising from the health status of patients in palliative care, the usability tests and implemented features received positive feedback from both patients and healthcare providers.ConclusionThe research findings have demonstrated the potential of digitisation in enhancing the quality of life for patients in palliative care. This was achieved through the implementation of patient-centred design, personalised care, the inclusion of social chatrooms and facilitating end-of-life discussions.