bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transposable elements and gene expression variation in the intraspecific hybrids of Capsella rubella
Authors: Chen Jia‐Fu , Xu Yong‐Chao , Jiang Juan , Niu Xiao‐Min , Hou Xing‐Hui , Zhang Zhi‐Qin , Guo Ya‐Long 
Published: July 2023

AbstractTransposable elements (TEs) are prevalent components of diverse genomes, and play an important role on the genomic stability and expression regulation of their adjacent genes. It is interesting to know the variation of TE expression and the effects of the presence/absence of TEs on gene expression after hybridization. Here we assessed the expression variation of TEs and the impacts of TEs on expression of nearby genes after hybridization based on comparisons of three pairs of reciprocal F1 hybrids and four parents in Capsella rubella. Of the 480 TE families expressed in all the four parents and six F1 hybrids, 7–23 (1.5%–4.2%) TE families were significantly differentially expressed between in silico and real F1 hybrids, indicating the expression levels of these TE families were affected during hybridization. In particular, there was a Copia TE superfamily and a non‐long terminal repeat (non‐LTR) TE differentially expressed between the reciprocal F1 hybrids of 879 and 86IT1, indicating maternal effects may have impacts on expression of TEs in these F1 hybrids. Besides the impacts on the expression of TE families of the hybridization, genes adjacent to polymorphic TEs tended to show a higher proportion (24.83%) of allele‐specific expression (ASE) in F1 hybrids. Overall, our results highlight the impacts of hybridization on the expression level variation of TEs, and the effects of TEs on ASE after hybridization.
A membrane protein of the rice pathogen Burkholderia glumae required for oxalic acid secretion and quorum sensing
Authors: Iqbal Asif , Nwokocha George , Tiwari Vijay , Barphagha Inderjit K. , Grove Anne , Ham Jong Hyun , Doerrler William T. 
Published: July 2023

AbstractBacterial panicle blight is caused by Burkholderia glumae and results in damage to rice crops worldwide. Virulence of B. glumae requires quorum sensing (QS)‐dependent synthesis and export of toxoflavin, responsible for much of the damage to rice. The DedA family is a conserved membrane protein family found in all bacterial species. B. glumae possesses a member of the DedA family, named DbcA, which we previously showed is required for toxoflavin secretion and virulence in a rice model of infection. B. glumae secretes oxalic acid as a “common good” in a QS‐dependent manner to combat toxic alkalinization of the growth medium during the stationary phase. Here, we show that B. glumae ΔdbcA fails to secrete oxalic acid, leading to alkaline toxicity and sensitivity to divalent cations, suggesting a role for DbcA in oxalic acid secretion. B. glumae ΔdbcA accumulated less acyl‐homoserine lactone (AHL) QS signalling molecules as the bacteria entered the stationary phase, probably due to nonenzymatic inactivation of AHL at alkaline pH. Transcription of toxoflavin and oxalic acid operons was down‐regulated in ΔdbcA. Alteration of the proton motive force with sodium bicarbonate also reduced oxalic acid secretion and expression of QS‐dependent genes. Overall, the data show that DbcA is required for oxalic acid secretion in a proton motive force‐dependent manner, which is critical for QS of B. glumae. Moreover, this study supports the idea that sodium bicarbonate may serve as a chemical for treatment of bacterial panicle blight.
Left bundle branch area pacing (LBBAP) Auto Threshold algorithms Evaluation for Conduction System Pacing: The LATECS pilot Trial
Authors: Ghiglieno Chiara , Dell'Era Gabriele , Veroli Alessandro , De Vecchi Federica , Santagostino Matteo , Porcellini Stefano , Patti Giuseppe 
Published: July 2023

AbstractBackgroundAutomated threshold measurements (ATM) and output adaptation improved safety and follow‐up of cardiac implantable devices (CIED) in the last years. These algorithms were validated for conventional cardiac pacing; however, they were not suitable for permanent His Pacing. Left bundle branch area pacing (LBBAP) is an emerging technique to obtain physiologic cardiac stimulation; we tried to assess if ATM could be applied to this setting.MethodsConsecutive patients receiving ATM‐capable CIED and LBBAP in our hospital were enrolled in this prospective, observational trial; they were evaluated 3 months after implant, comparing pacing thresholds manually assessed and obtained via ATM. Subsequent remote follow‐up was carried on when available.ResultsForty‐five patients were enrolled. ATM for LBBAP lead provided consistent results in all the patients and was therefore activated; mean value of manually obtained LBBAP capture threshold was 0.66 ± 0.19 V versus ATM of 0.64 ± 0.19 V. TOST analysis showed equivalence of the two measures (p = .66). At subsequent follow‐up (mean follow up 7.7 ± 3.2 months), ATM was effective in assessing pacing thresholds and no clinical adverse event was observed.ConclusionsATM algorithms proved equivalent to manual testing in determining capture threshold and were reliably employed in patients receiving LBBAP CIED.
Management of treatment‐resistant nocturnal enuresis
Authors: Tsuji Shoji , Kaneko Kazunari 
Published: July 2023

AbstractNocturnal enuresis is defined as intermittent urinary incontinence during sleep in children 5 years of age and older, occurring at least once a month for at least 3 months. In Japan, pediatricians who do not specialize in nocturnal enuresis have become more proactive in treating the condition since 2016, when the guidelines for treating it were revised for the first time in 12 years. For monosymptomatic nocturnal enuresis, the first step is lifestyle guidance, with a focus on the restriction of fluid intake at night; however, if lifestyle guidance does not decrease the frequency of nocturnal enuresis, aggressive treatment should be added. The first choice of aggressive treatment is oral desmopressin, an antidiuretic hormone preparation, or alarm therapy. However, there remain patients whose wet nights do not decrease with oral desmopressin or alarm therapy. In such cases, it is necessary to reconfirm the method of desmopressin administration and check for factors that may decrease the efficacy of desmopressin. If alarm therapy does not increase the number of dry nights, it is possible that the patient is fundamentally unsuitable for alarm therapy. If dry nights do not increase with oral desmopressin or alarm therapy, the next treatment strategy should be considered immediately to keep the patient motivated for treatment.
Prevalence of smoking during pregnancy and associated social inequalities in developed countries over the 1995–2020 period: A systematic review
Authors: Bonello Kim , Figoni Hugo , Blanchard Estelle , Vignier Nicolas , Avenin Guillaume , Melchior Maria , Cadwallader Jean‐Sébastien , Chastang Julie , Ibanez Gladys 
Published: July 2023

AbstractBackgroundSmoking during pregnancy (SDP) is an important source of preventable morbidity and mortality for both mother and child.ObjectivesThe aim of this study was to describe changes in the prevalence of SDP over the last 25 years in developed countries (Human Development Index >0.8 in 2020) and associated social inequalities.Data sourcesA systematic review was conducted based on a search in PubMed, Embase and PsycInfo databases and government sources. Study selection and data extraction: Published studies between January 1995 and March 2020, for which the primary outcome was to assess the national prevalence of SDP and the secondary outcome was to describe related socio‐economic data were included in the analysis. The selected articles had to be written in English, Spanish, French or Italian.SynthesisThe articles were selected after successive reading of the titles, abstracts and full‐length text. An independent double reading with intervention of a third reader in case of disagreement allowed including 35 articles from 14 countries in the analysis.ResultsThe prevalence of SDP differed across the countries studied despite comparable levels of development. After 2015, the prevalence of SDP ranged between 4.2% in Sweden and 16.6% in France. It was associated with socio‐economic factors. The prevalence of SDP slowly decreased over time, but this overall trend masked inequalities within populations. In Canada, France and the United States, the prevalence decreased more rapidly in women of higher socio‐economic status, and inequalities in maternal smoking were more marked in these countries. In the other countries, inequalities tended to decrease but remained significant.ConclusionsDuring pregnancy, that is a period described as a window of opportunity, smoking and social vulnerability factors need to be detected to implement targeted prevention strategies aiming at reducing related social inequalities.
Toward an effective policy mix for domestic waste management in China: Interdepartmental cooperation as an enabler
Authors: Zhang Jingjing , Luo Guifang , Yan Yan 
Published: July 2023

AbstractThe approach of combining diverse policy instruments is increasingly being applied to address complex environmental and sustainability issues. For such an approach of policy mix to be effective, broader contextual and process factors must be taken into account. In this paper, we identify interdepartmental cooperation within the government as a condition for an effective policy mix, especially for China. Taking domestic waste governance as a case study, we collect policy document data from 32 cities across China in the last decade and verify the influential mechanism between the interdepartmental cooperation network and the policy mix through quantitative methods of social network analysis and regression. The empirical results show that the Chinese government has intensified interdepartmental cooperation over the past decade in the area of domestic waste governance, which has facilitated the increased adoption of policy mixes. In addition, a shift in the policy strategy and policy direction of the Chinese government for domestic waste governance can be traced from the changes in the specific departments involved in the cooperation network.
Understanding nursing personnel's health while working in end‐of‐life care—A hermeneutical study
Authors: Karlsson Margareta , Pennbrant Sandra , Kasén Anne 
Published: July 2023

AbstractAimThis study aimed to explore nursing personnel's health while working in end‐of‐life care.IntroductionEnd‐of‐life care is challenging both for nursing personnel and for the healthcare organisation, as retaining nursing staff is difficult. Although end‐of‐life care involves the risk of burnout, it also encompasses protective factors that can lead to personal and professional development and satisfaction, and that can enable personnel to encounter their own inner selves. In order to focus on the health of nursing personnel we chose the theory of caritative caring as our theoretical perspective.MethodA qualitative inductive research design with a hermeneutical approach was chosen to explore nursing personnel's health while working in end‐of‐life care. Two assistant nurses and six registered nurses with experience in end‐of‐life care at a palliative care unit participated. The study was approved by a Regional Ethical Review Board.ResultsThe results are presented on three levels: rational, structural and existential. In the rational level, fellowship and togetherness with colleagues, as well as being able to distinguish between private life and work were important for nursing personnel's strategies for maintaining their health. At the structural level, social togetherness, sharing emotions and being involved in each other's emotions were important for nursing personnel's health. The existential level showed that the nursing personnel's own existential situation was affected when their inner self was emotionally affected by the patients' suffering. The awareness of suffering, life and death made the nursing personnel feel inner security, both as nursing professionals and as human beings.ConclusionA common perspective based on a theory of caritative care may be helpful for retaining nursing personnel. While the study highlights nursing personnel's health while working in an end‐of‐life care context, the results may also be applicable to nursing professionals' health in other contexts.
Treatment with add-on IVIg in Myositis Early In the diSease course May be sUperior to Steroids alone for reaching CLinical improvEment (TIME IS MUSCLE): study protocol of a phase-2 double-blind placebo-controlled randomised trial
Authors: Kamperman Renske G , Bogaards Johannes A , Evers Sanne W , Walter Hannah A W , de Visser Marianne , de Borgie Corianne , Colen - de Koning Jantine C A , Verhamme Camiel , Maas Mario , Eftimov Filip , van Schaik Ivo N , van der Kooi Anneke J , Raaphorst Joost 
Published: July 2023

IntroductionFor idiopathic inflammatory myopathies (IIM) (‘myositis’) standard initial treatment is high-dosed glucocorticoids, which results in relatively slow improvement of muscle strength. Early immunosuppression or modulation by intensive treatment (‘hit-early, hit-hard’) may induce faster reduction of disease activity and prevent chronic disability due to disease-induced structural muscle damage. Intravenous immunoglobulin (IVIg) in addition to standard glucocorticoid treatment may be promising in this regard as was shown in various studies: add-on IVIg improved symptoms and muscle strength in refractory myositis patients and monotherapy IVIg improved outcomes after 9 weeks, in about half of treatment-naive patients.HypothesisWe hypothesise that early add-on IVIg leads to a greater clinical response after 12 weeks in patients with newly diagnosed myositis, in comparison to prednisone monotherapy. Second, we expect that early treatment with add-on IVIg leads to a faster time to improvement and sustained positive effects on multiple secondary outcomes.MethodsThe Time Is Muscle trial is a phase-2 double-blind placebo-controlled randomised trial. Forty-eight patients with IIM will be treated with IVIg or placebo at baseline (within 1 week after diagnosis) and after 4 and 8 weeks, in addition to standard therapy with prednisone. The primary outcome is the Total Improvement Score (TIS) of the myositis response criteria at 12 weeks. At baseline, and after 4, 8, 12, 26 and 52 weeks, relevant secondary outcomes will be assessed, including time to moderate improvement (TIS≥40), mean daily prednisone dosage, physical activity, health-related quality of life, fatigue and MRI muscle imaging parameters.Ethics and disseminationEthical approval was obtained from the medical ethics committee of the Academic Medical Centre, University of Amsterdam, the Netherlands (2020_180; including a first amendment approval at the 12 April 2023; A2020_180_0001). The results will be distributed through conference presentations and peer-reviewed publications.Trial registration numberEU Clinical trials register (2020-001710-37).
Determinants of early initiation of breast feeding during COVID-19 pandemic among urban-dwelling mothers from Tigray, Northern Ethiopia: a community-based cross-sectional study
Authors: Gebretsadik Gebretsadkan Gebremedhin , Berhe Kidanemaryam , Gebregziabher Hadush 
Published: July 2023

ObjectivesThe objective of this study was to assess the practice of early initiation of breast feeding (EIBF) and its determinant factors among urban-dwelling mothers from Tigray during the COVID-19 pandemic.DesignA community-based cross-sectional study was conducted from April to June 2021. Data were analysed using StataSE Version 16 software. To identify determinant factors of the dependent variable, multivariate logistic regression analyses was used at a statistical significance of p<0.05. The strength of the association was measured by OR and 95% CI.Setting and participantsThe study was conducted among 633 lactating mothers of infants under the age of 6 months living in Mekelle city, Tigray, Northern Ethiopia from April to June 2021. A three-stage cluster sampling was used to select study participants.Main outcome measureEIBF or no EIBF.ResultsThree hundred and sixty eight (59.6%) mothers/caregivers practised EIBF. Maternal education (adjusted OR, AOR 2.45, 95 % CI 1.01 to 5.88), parity (AOR 1.20, 95 % CI 1.03 to 2.20), caesarean section delivery (AOR 0.47, 95 % CI 0.32 to 0.69) and breastfeeding information and support postdelivery (AOR 1.59, 95% CI 1.10 to 2.31) were found to be significant determinants of EIBF.ConclusionsEIBF is defined as initiation of breastfeeding within 1 hour after delivery. EIBF practice was far from optimal. During the COVID-19 pandemic, maternal education, parity, type of delivery and the provision of up-to-date breastfeeding information and support right after delivery determined the time of breastfeeding initiation.
Home-based optimal newborn care practice and associated factors among mothers in Ethiopia: a community-based longitudinal panel survey
Authors: Mohammed Shikur , Worku Alemayehu , Girma Eshetu 
Published: July 2023

ObjectivesThe objective of this study was to determine the level of home-based optimal newborn care practice and associated factors among mothers in Ethiopia.DesignA community-based longitudinal panel survey design.Setting, participants and outcomesWe used data from the Performance Monitoring for Action Ethiopia panel survey (2019–2021). A total of 860 mothers of neonates were included in the analysis. A generalised estimating equation logistic regression model was used to identify factors associated with home-based optimal newborn care practice and to account for the clustering nature of the data by enumeration area. An OR with 95% CI was used to measure the association between exposure and outcome variables.ResultsThe level of home-based optimal newborn care practice was 8.7% with 95% uncertainty interval ranging from 6% to 11%. After adjusting the effect of potential confounding factors, area of residence remained statistically significantly associated with mothers’ optimal newborn care practice. The chance of home-based optimal newborn care practice was 69% times lower among mothers from rural areas compared with those in urban areas (adjusted OR=0.31, 95% CI=0.15, 0.61).ConclusionThe findings of this study showed that the level of home-based optimal newborn care practice was very low in Ethiopia. Also, home-based optimal newborn care practice was lower among mothers from rural areas in the nation. Therefore, health planners and healthcare providers, including health extension workers, should give priority attention to mothers from rural areas to improve their optimal newborn care practice by considering their context-specific factors or barriers.
Cohort profile: Ngā Kawekawe o Mate Korona | Impacts of COVID-19 in Aotearoa – a prospective, national cohort study of people with COVID-19 in New Zealand
Authors: Russell Lynne , Jeffreys Mona , Churchward Marianna , Cumming Jackie , McKenzie Fiona , O'Loughlin Claire , Asiasiga Lanuola , Bell Rebecca , Hickey Huhana , Irurzun-Lopez Maite , Kamau Laura , Kokaua Jesse , McDonald Janet , McFarland-Tautau Myra , Smiler Kirsten , Uia Tali , Vaka Sione , Veukiso-Ulugia Analosa , Wong Conroy , Ellison Loschmann Lis 
Published: July 2023

PurposeThe COVID-19 pandemic has had significant health, social and economic impacts around the world. We established a national, population-based longitudinal cohort to investigate the immediate and longer-term physical, psychological and economic impacts of COVID-19 on affected people in Aotearoa New Zealand (Aotearoa), with the resulting evidence to assist in designing appropriate health and well-being services for people with COVID-19.ParticipantsAll people residing in Aotearoa aged 16 years or over, who had a confirmed or probable diagnosis of COVID-19 prior to December 2021, were invited to participate. Those living in dementia units were excluded. Participation involved taking part in one or more of four online surveys and/or in-depth interviews. The first wave of data collection took place from February to June 2022.Findings to dateBy 30 November 2021, of 8735 people in Aotearoa aged 16+ who had COVID-19, 8712 were eligible for the study and 8012 had valid addresses so were able to be contacted to take part. A total of 990 people, including 161 Tāngata Whenua (Māori, Indigenous peoples of Aotearoa) completed one or more surveys; in addition, 62 took part in in-depth interviews. Two hundred and seventeen people (20%) reported symptoms consistent with long COVID. Key areas of adverse impacts were experiences of stigma, mental distress, poor experiences of health services and barriers to healthcare, each being significantly more pronounced among disabled people and/or those with long COVID.Future plansFurther data collection is planned to follow-up cohort participants. This cohort will be supplemented by the inclusion of a cohort of people with long COVID following Omicron infection. Future follow-ups will assess longitudinal changes to health and well-being impacts, including mental health, social, workplace/education and economic impacts of COVID-19.
Kinesiophobia and its associated factors in patients with coronary heart disease: a cross-sectional study based on latent feature analysis
Authors: Wang Zijiao , Zhang Yifan , Wang Yanping , Liu Lamei , Zhang Junmei 
Published: July 2023

ObjectivesThe aim of this study was to explore the current situation of kinesiophobia in patients with coronary heart disease, classify it based on potential profile analysis and explore the relevant factors of kinesiophobia in different categories of patients with coronary heart disease.DesignCross-sectional study.SettingPatients with coronary heart disease in China.ParticipantsAdult (aged >18 years) patients with coronary heart disease in China; 252 participants in this study answered the questionnaire.Primary and secondary outcome measuresThe study investigated the scores of Tampa Scale for Kinesiophobia Heart, and collected information on the patient’s age, gender, monthly household income, education level, place of residence, marital status, occupational status, hypertension, diabetes, heart failure and body mass index (BMI).ResultsKinesiophobia in patients with coronary heart disease can be divided into low fear type (C1), intermediate fear type (C2) and high fear type (C3). Elderly patients were classified as type C3. Women and patients with a normal BMI were classified as type C1; patients with a normal BMI and patients with an overweight BMI were classified as type C2.ConclusionKinesiophobia of patients with coronary heart disease can be divided into three categories, and intervention measures are implemented according to their different demographic characteristics to reduce kinesiophobia of patients and promote the participation of patients in exercise rehabilitation.