bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid system
Authors: Cui Sheng , Fang Xianying , Lee Hanbi , Shin Yoo Jin , Koh Eun-Sil , Chung Sungjin , Park Hoon Suk , Lim Sun Woo , Lee Kang In , Lee Jae Young , Yang Chul Woo , Chung Byung Ha 
Published: February 2023

Abstract
Objectives
To explore the possibility of kidney organoids generated using patient derived human induced pluripotent stem cells (hiPSC) for modeling of Fabry disease nephropathy (FDN).

Methods
First, we generated hiPSC line using peripheral blood mononuclear cells (PBMCs) from two male FD-patients with different types of GLA mutation: a classic type mutation (CMC-Fb-001) and a non-classic type (CMC-Fb-003) mutation. Second, we generated kidney organoids using wild-type (WT) hiPSC (WTC-11) and mutant hiPSCs (CMC-Fb-001 and CMC-Fb-003). We then compared alpha-galactosidase A (α-GalA) activity, deposition of globotriaosylceremide (Gb-3), and zebra body formation under electromicroscopy (EM).

Results
Both FD patients derived hiPSCs had the same mutations as those detected in PBMCs of patients, showing typical pluripotency markers, normal karyotyping, and successful tri-lineage differentiation. Kidney organoids generated using WT-hiPSC and both FD patients derived hiPSCs expressed typical nephron markers without structural deformity. Activity of α-GalA was decreased and deposition of Gb-3 was increased in FD patients derived hiPSCs and kidney organoids in comparison with WT, with such changes being far more significant in CMC-Fb-001 than in CMC-Fb-003. In EM finding, multi-lammelated inclusion body was detected in both CMC-Fb-001 and CMC-Fb-003 kidney organoids, but not in WT.

Conclusions
Kidney organoids generated using hiPSCs from male FD patients might recapitulate the disease phenotype and represent the severity of FD according to the GLA mutation type.
Delineation of recurrent glioblastoma by whole brain spectroscopic magnetic resonance imaging
Authors: Bell Jonathan B. , Jin William , Goryawala Mohammed Z. , Azzam Gregory A. , Abramowitz Matthew C. , Diwanji Tejan , Ivan Michael E. , del Pilar Guillermo Prieto Eibl Maria , de la Fuente Macarena I. , Mellon Eric A. 
Published: February 2023

Abstract
Background
Glioblastoma (GBM) cellularity correlates with whole brain spectroscopic MRI (sMRI) generated relative choline to N-Acetyl-Aspartate ratio (rChoNAA) mapping. In recurrent GBM (rGBM), tumor volume (TV) delineation is challenging and rChoNAA maps may assist with re-RT targeting.

Methods
Fourteen rGBM patients underwent sMRI in a prospective study. Whole brain sMRI was performed to generate rChoNAA maps. TVs were delineated by the union of rChoNAA ratio over 2 (rChoNAA > 2) on sMRI and T1PC. rChoNAA > 2 volumes were compared with multiparametric MRI sequences including T1PC, T2/FLAIR, diffusion-restriction on apparent diffusion coefficient (ADC) maps, and perfusion relative cerebral blood volume (rCBV).

Results
rChoNAA > 2 (mean 27.6 cc, range 6.6–79.1 cc) was different from other imaging modalities (P ≤ 0.05). Mean T1PC volumes were 10.7 cc (range 1.2–31.4 cc). The mean non-overlapping volume of rChoNAA > 2 and T1PC was 29.2 cm3. rChoNAA > 2 was 287% larger (range 23% smaller–873% larger) than T1PC. T2/FLAIR volumes (mean 111.7 cc, range 19.0–232.7 cc) were much larger than other modalities. rCBV volumes (mean 6.2 cc, range 0.2–19.1 cc) and ADC volumes were tiny (mean 0.8 cc, range 0–3.7 cc). Eight in-field failures were observed. Three patients failed outside T1PC but within rChoNAA > 2. No grade 3 toxicities attributable to re-RT were observed. Median progression-free and overall survival for re-RT patients were 6.5 and 7.1 months, respectively.

Conclusions
Treatment of rGBM may be optimized by sMRI, and failure patterns suggest benefit for dose-escalation within sMRI-delineated volumes. Dose-escalation and radiologic-pathologic studies are underway to confirm the utility of sMRI in rGBM.
Functional heterogeneity of MCT1 and MCT4 in metabolic reprogramming affects osteosarcoma growth and metastasis
Authors: Sheng Gaohong , Gao Yuan , Wu Hua , Liu Yang , Yang Yong 
Published: February 2023

Abstract
Background
Osteosarcoma is the most common primary malignant bone tumor in adolescents and children and prone to develop lung metastasis. Its prognosis has been virtually unimproved over the last few decades, especially in patients with metastases, who suffer from a dismal survival. Recently, increasing attention has been devoted to monocarboxylate transporters-related (MCTs) metabolic reprogramming. However, the role of MCT1 and MCT4 in osteosarcoma progression and the underlying mechanisms remain to be further elucidated.

Methods
In this study, we established MCT1 and/or MCT4 knockout cell lines by CRISPR/Cas9 genome editing technology. Then, we assessed glycolysis and oxidative phosphorylation capacities by measuring lactate flux and oxygen consumption. We also performed flowcytometry to test circulating tumor cells and PET/CT to evaluate glucose uptake.

Results
MCT1 was found to be involved in both glycolysis and oxidative respiration due to its ability to transport lactate in both directions. MCT1 inhibition significantly reduced circulating tumor cells and distant metastases partially by increasing oxidative stress. MCT4 was primarily related to glycolysis and responsible for lactate export when the concentration of extracellular lactate was high. MCT4 inhibition dramatically suppressed cell proliferation in vitro and impaired tumor growth with reduction of glucose uptake in vivo.

Conclusions
Our results demonstrate the functional heterogeneity and redundancy of MCT1 and MCT4 in glucose metabolism and tumor progression in osteosarcoma. Thus, combined inhibition of MCT1 and MCT4 may be a promising therapeutic strategy for treating tumors expressing both transporters.
What if the devil is my guardian angel: ChatGPT as a case study of using chatbots in education
Authors: Tlili Ahmed , Shehata Boulus , Adarkwah Michael Agyemang , Bozkurt Aras , Hickey Daniel T. , Huang Ronghuai , Agyemang Brighter 
Published: February 2023

AbstractArtificial Intelligence (AI) technologies have been progressing constantly and being more visible in different aspects of our lives. One recent phenomenon is ChatGPT, a chatbot with a conversational artificial intelligence interface that was developed by OpenAI. As one of the most advanced artificial intelligence applications, ChatGPT has drawn much public attention across the globe. In this regard, this study examines ChatGPT in education, among early adopters, through a qualitative instrumental case study. Conducted in three stages, the first stage of the study reveals that the public discourse in social media is generally positive and there is enthusiasm regarding its use in educational settings. However, there are also voices who are approaching cautiously using ChatGPT in educational settings. The second stage of the study examines the case of ChatGPT through lenses of educational transformation, response quality, usefulness, personality and emotion, and ethics. In the third and final stage of the study, the investigation of user experiences through ten educational scenarios revealed various issues, including cheating, honesty and truthfulness of ChatGPT, privacy misleading, and manipulation. The findings of this study provide several research directions that should be considered to ensure a safe and responsible adoption of chatbots, specifically ChatGPT, in education.
The Individual vs. the State
Authors: Juruś Dariusz 
Published: February 2023

The paper presents the profiles of three American thinkers associated with the tradition of individualist anarchism. These will be: Lysander Spooner (1808–1887), Albert Jay Nock (1870–1945) and Murray Newton Rothbard (1926–1995). These thinkers were involved not only in writing, but were also active participants in the political life of the time. In their opinion, the state, whose genesis is based on violence and conquest, and the individual are the greatest enemies. The state was perceived as the greatest threat to the freedom of the individual, as it violated the principles of natural law, which in turn was to constitute the moral foundation of society. The state was, therefore, in the opinion of these thinkers, something inherently unjust, because by force and against the will of its citizens it forced them to obey. They did not perceive history in terms of class struggle, but as a struggle between individuals and society against the state. Spooner compared the state to a gang of robbers and murderers (valuing them higher than the state, however) and argued that the constitution did not bind citizens in any way, as it had not been signed by them personally, and the government had no power over the one handed over to it by free units. Nock pointed to the gradual appropriation by the state (“professional criminals”) of competences belonging to society, and the opposing goals of both. He distinguished between a government that aims to protect individuals and justice, and a state that aims to plunder, based on a law it has created. Rothbard advocated the abolition of the state as a tax consumer and its replacement with an anarcho-capitalist order in which private property would be its foundation.
Theories of Spooner, Nock and Rothbard, although directed against the state, were not only negative. At their roots was the good of individuals. For Spooner, they were primarily workers, for Nock, citizens, and for Rothbard, entrepreneurs and owners. This fact is worth emphasizing, as the criticism of anarchism often boils down to allegations of radicalism and utopianism, without taking into account its protectionist nature. Criticism of the state is always a consequence of the human vision and should be considered from this perspective.
The concepts of Spooner, Nock and Rothbard, although directed against the state, were not only negative. At their root was the good of individuals. For Spooner, they were primarily workers, for Nock, citizens, and for Rothbard, entrepreneurs and owners. This fact is worth emphasizing, as the criticism of anarchism often boils down to allegations of radicalism and utopianism, without taking into account its protectionist nature. In the article, in addition to presenting the positions of American individualist anarchists, we will pay attention to the positive aspects of the criticism of the state and show that already in the nineteenth and twentieth century anarchists recognized certain mechanisms of power, which also occur with particular intensity today.
TWEET SENTIMENT ANALYSISON GREENSPACES
Authors: Denaro Lino Garda , Sujana Yudianto , Fatihul Ilmy Hafsah 
Published: February 2023

Twitter has become one of the most significant resources for text mining. Twitter can provide information about human activities, mobility, and emotional patterns along with location data. Many types of text research can be made with these data, one of which is sentiment analysis. This study evaluates the potential of deriving emotional responses of individuals from tweets while they experience and interact with urban green space. A machine learning model using Support Vector Machine (SVM) and corpus from over 2000 movie reviews has been made. This model is used to classify incoming tweets into positive and negative sentiments. Then the web-based recommender system has been built to provide suggestions for green spaces based on users' preferred activities.
Revisión sobre aspectos bioéticos en las decisiones de triaje en la Unidad de Cuidados Intensivos durante la pandemia por COVID-19
Authors: García León Grecia Alejandra , Lima Sanchez Dania Nimbe , Abascal Miguel Lucía 
Published: February 2023

Introducción: La pandemia por COVID-19 provocó un aumento en los pacientes canalizados a las Unidades de Cuidados Intensivos (UCI). La aplicación de algoritmos de triaje provocó dilemas éticos cuando se tenía que decidir sobre el manejo clínico y/o el tratamiento de los pacientes. Objetivos: Analizar los principales aspectos bioéticos involucrados en los algoritmos usados en la toma de decisiones de triaje en la UCI durante la pandemia por COVID-19 a partir de una revisión de la literatura publicada en el periodo comprendido desde julio del año 2020 hasta febrero del año 2021. Métodos: Se realizó una búsqueda en Pubmed, SciELO, Ovid y Cochrane con los términos de búsqueda en inglés y español: “triage” (triaje), “Covid”, “SARS Cov-2”, “unidad de cuidados intensivo”, “ética”. Se excluyeron los artículos que no mencionaron algoritmos de intervención en la UCI, que no analizan la toma de decisiones, que no implican el entorno clínico, que no estuvieran indexados o artículos repetidos. Resultados: Se obtuvieron 45 artículos: los resúmenes fueron revisados de forma independiente por dos autores para eliminar sesgos, seleccionando 12 artículos que cumplían con los criterios de selección. Se encontró que los principales aspectos éticos que se tomaron en cuenta fueron: priorización inevitable, justicia y apoyo al rol del juicio clínico tomando en cuenta los derechos personales. Conclusiones: Es necesario realizar ajustes bioéticos para estandarizar la universalización de la toma de decisiones en momentos donde la capacidad de los servicios de salud se ve rebasada.
Responsabilidad civil médica en telemedicina: una propuesta de principios para una lex artis telemédica
Authors: Lecaros Urzúa Juan Alberto , López Gaete Gonzalo Eugenio 
Published: February 2023

El presente artículo analiza la responsabilidad civil médica atribuible a los profesionales de la salud que intervienen en el proceso de atención a distancia. Con este objeto, se propone un conjunto de principios de los cuales se derivan deberes de cuidado en la telemedicina exigibles a los profesionales de salud y a los prestadores. A partir de los principios de analogía con y complementariedad de la atención presencial, se desarrollan tres categorías de principios especiales para la atención a distancia: principios habilitantes, principios operativos y principios de protección al paciente. Bajo este esquema de principios se especifican deberes de cuidado, distinguiendo aquellos que responden a los riesgos causados por la distancia física de aquellos que surgen de los cambios organizacionales necesarios para este tipo de atención. La aplicación de estos deberes se analiza en tres escenarios de telemedicina: la teleconsulta, la teleinterconsulta y la teleconsultoría. Esta estructura de principios y deberes en la prestación de salud a distancia permite delimitar los alcances de la lex artis para cada escenario específico y, al mismo tiempo, elaborar estrategias regulatorias considerando criterios de necesidad, racionalidad y suficiencia, según los contenidos específicos que caracterizan cada escenario de atención de salud a distancia.
Patients présentant une pathologie chronique et soins palliatifs en oncologie: Une mise en relation trop tardive. Télémédecine pour les soins palliatifs en oncologie
Authors: Couderc Bettina , Mauries Saffon Valerie , Faya-Robles Alfonsina 
Published: February 2023

La population française comprendra 23,4 % de personnes de plus de 65 ans en 2030. La plupart d’entre elles présentera des pathologies chroniques (cancers) nécessitant une prise en charge globale par les équipes de soins palliatifs. Toutefois le nombre restreint de lits d’hospitalisation et de médecins suggère que l’on aura rapidement besoin de developper la télémédecine pour le suivi des patients en soins palliatifs Nous avons proposé à 30 patients un suivi mensuel par téléconsultation après une première évaluation en Hospitalisation De Jour de soins palliatifs afin d’évaluer la faisabilité matérielle des consultations par télémédecine (patients altérés dans le cadre de leur parcours avancé dans la maladie cancéreuse, souvent âgés) et l’adhésion des patients dans cette pratique. Nous avons recueilli le vécu et ressenti des patients par rapport à ces téléconsultations 3 mois post inclusion. Nous avons montré la faisabilité de la téléconsultation pour les patients suivis en soins palliatifs. Les patients sont favorables à ce type de suivi. Ils n’ont aucune difficulté à se connecter indépendamment de leur âge. Tous en tirent bénéfice. Une relation de confiance s’établit facilement avec leur médecin, malgré leur méconnaissance antérieure à la téléconsultation. En revanche, notre étude met en évidence que 65% des patients à qui nous avons proposé un suivi par télémédecine sont décédés avant les 3 mois post inclusion traduisant un accès beaucoup trop tardif des patients aux soins palliatifs. Il est donc particulièrement important de promouvoir non seulement la télémédecine mais également un accès précoce aux soins palliatifs des patients.
Finite Element Modelling of Laser-Induced Hole Formation in Optical Storage Media
Authors: Evans K.E. , Burgess A.N. , Abbott S.J. 
Published: February 2023

Video discs and compact discs are examples of modern optical storage techniques that use lasers to read from permanently recorded storage media, capable of storing large quantities (gigabytes) of digitised information. Current research is involved in the development of archival media ('Write-Once-Read-Many' times systems) and erasable optical media. Here locallised laser heating is used to create small pits (for WORM systems) or a locallised reversible change in the medium (in erasable systems) to form the basic 'bit' of information. The aim is to determine the optimum combination of materials for this process to produce the highest data density, minimum error rate, and maximum read and write rates. This will be achieved by accurately controlling pit size, increasing the sensitivity of the film to pit formation and providing a sharp threshold response to the laser.
Due to the extreme difficulties in observing the process of pit formation, which involves a micron-sized laser beam creating temperatures of over 1000°C in a time of less than 100 nsecs, finite element methods are required to model the process (a) to try and detect the process of pit formation (b) to optimise the combination of materials used. Currently, practical systems use either a tellurium alloy or an organic dye on a polycarbonate or PMMA substrate. Various complex layer structures have been tried to maximise energy absorption in the storage medium.
A finite element model will be described which has been used to solve the transient thermal conduction equation, with a time dependent heat source, to accurately model the temperature variation in a number of different storage media. The resultant temperatures are then used to determine stress distributions in the storage medium. The variation of temperature and stress is required to determine the mechanism of pit formation in the medium. It will be shown how a better understanding of the processes involved can lead to a significant change in the materials used by a close examination of the most important physical parameters.
Demonstration - Cleaning Optics
Authors: Gourley Helen , Aiello Theresa , Brown Norman , Chupp Vern , Giove Joseph , Gourley Darrell , Handy Tom 
Published: February 2023

This demonstration session brought together a group of optical cleaning problems. They were:
1. Cleaning a Brewster's angle polarizer before and after coating.
2. Cleaning a Master Optic before replication.
3. Cleaning a laser amplifier disc immediately before assembly.
4. Cleaning a lens in its metal holder.
Participants demonstrated their techniques, special tools and materials.
Optimisation Procedures Using the "Eye" Merit Function
Authors: Piegari Angela , Emiliani Gabriele 
Published: February 2023

In the design of optical coatings the desired performance is continuously compared with the calculated values.
When the comparison between two curves is made over a wide wavelength range an immediate evaluation of how much the calculated curve approaches the desired one can be obtained simply overlappping the two graphs and looking at them. That means the eye makes its own total evaluation that in most cases represents the best judgement. In general the similarity of two curves is evaluated by a merit function that compares them in a number of chosen points (wavelengths) where the difference between the two values is calculated. The choice of these points and of their number is often very critical and requires a great experience.
In-Cylinder LDA Measurements Using a Correlator
Authors: Max E. , Denbratt I. 
Published: February 2023

An LDA system using photon correlation technique and capable of handling non photon resolved signals is reported. Comparisons with the power spectrum of actual detector signals show that the correlation technique is very efficient.
Turbulence production by means of swirl has been tested by making cycle resolved LDA measurements in a motored petrol engine for three different swirl levels. Firing tests of the engine show that the ignition delay (0 - 1% burned) correlates well with the high frequency components (> 1 kHz) of the turbulence at the spark plug location.
Prevalence of human infection with respiratory adenovirus in China: A systematic review and meta-analysis
Authors: Liu Mei-Chen , Xu Qiang , Li Ting-Ting , Wang Tao , Jiang Bao-Gui , Lv Chen-Long , Zhang Xiao-Ai , Liu Wei , Fang Li-Qun 
Published: February 2023

Background
Human adenovirus (HAdV) is a major pathogen that causes acute respiratory tract infections (ARTI) and is frequently associated with outbreaks. The HAdV prevalence and the predominant types responsible for ARTI outbreaks remains obscure in China.

Methods
A systematic review was performed to retrieve literature that reported outbreaks or etiological surveillance of HAdV among ARTI patients in China from 2009 to 2020. Patient information was extracted from the literature to explore the epidemiological characteristics and clinical manifestations of the infection of various HAdV types. The study is registered with PROSPERO, CRD42022303015.

Results
A total of 950 articles (91 about outbreaks and 859 about etiological surveillance) meeting the selection criteria were included. Predominant HAdV types from etiological surveillance studies differed from those in outbreak events. Among 859 hospital-based etiological surveillance studies, positive detection rates of HAdV-3 (32.73%) and HAdV-7 (27.48%) were significantly higher than other virus types. While nearly half (45.71%) of outbreaks were caused by HAdV-7 with an overall attack rate of 22.32% among the 70 outbreaks for which the HAdVs were typed by the meta-analysis. Military camp and school were main outbreak settings with significantly different seasonal pattern and attack rate, where HAdV-55 and HAdV-7 were identified as the leading type, respectively. Clinical manifestations mainly depended on the HAdV types and patient’s age. HAdV-55 infection tends to develop into pneumonia with poorer prognosis, especially in children <5 years old.

Conclusions
This study improves the understanding of epidemiological and clinical features of HAdV infections and outbreaks with different virus types, and helps to inform future surveillance and control efforts in different settings.