bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral and nasal provocation test in chicken egg allergy—case report
Authors: Krzych-Fałta E. , Wojas O. , Samel-Kowalik P. , Samoliński B. , Sybilski A. , Białek S. 
Published: August 2023

Abstract
Introduction
Allergy to chicken egg protein is a common form of food allergy. The most common clinical presentation includes gastrointestinal, skin, and respiratory symptoms. Differential diagnosis, including provocative tests, is critical in diagnosis.

Case description
We present a case of a 21-year-old patient with egg allergy, who underwent a double-blind food provocation test with placebo (evaluating subjective complaints from the gastrointestinal tract) and a titrated nasal provocation test using dry chicken egg content. We assessed the response of the nasal mucosa in the provocation test using the visual analogue scale (VAS), acoustic and optical rhinometry, as well as measurements of nitric oxide concentration in the exhaled air. During the provocation test, we measured the changes in the transverse section of the nasal passages, which were accompanied by subjective complaints measured with the VAS scale, using objective techniques. In the nasal provocation test with a dose of 20 µg of chicken egg protein, we observed an increase in the reactivity of the nasal mucosa and a decrease in the level of nitric oxide in the exhaled air from the upper airways (920 ppb before the provocation test and up to 867 ppb during the early stage of the allergic reaction). During the provocation tests, we recorded typical symptoms associated with the early stage of the allergic reaction; including nasal obstruction (1.2 cm), leakage of watery discharge (0.8 cm) in the food test, and itchy nose (1.1 cm) in the food test vs. the nasal test: 4.6, 2.8, and 3.5 cm, respectively.

Conclusions
The nasal mucosa provides convenient conditions for evaluation of the severity of allergy to common food allergens, including chicken egg allergens.
Simulating the diameter growth responses of Larix gmelini Rupr. and Betula platyphylla Suk. to biotic and abiotic factors in secondary forests in Northeast China
Authors: Wang Tao , Xie Longfei , Miao Zheng , Dong Lihu , Hao Yuanshuo , Ma Aiyun , Li Fengri 
Published: August 2023

Abstract
Key message
The diameter growth of Dahurian larch (Larix gmelini Rupr.) and white birch (Betula platyphylla Suk.) species in secondary forest of Northeast China was not only influenced by biological factors such as tree size and stand characteristics, but also significantly affected by topographic and climatic factors such as temperature and precipitation. It is necessary to consider the abiotic factors in simulating the diameter growth.

Context
Climate change, such as global temperature rise, increased frequency of extreme weather events, and rising sea levels, has put forest ecosystems in an unstable state and has an impact on species composition, growth harvest, productivity and other functions of forests. And this impact varies in climate scenarios, regions and forest types.

Aims
To gain a comprehensive understanding of the adaptation for key species to their environment in secondary forests in Northeast China, the diameter growth responses of Dahurian larch and white birch to biotic and abiotic factors were simulated to assess the effects of climate on diameter growth.

Methods
China’s National Forest Continuous Inventory (NFCI) data from 2005 to 2015 were used to develop linear mixed-effects diameter growth models with plot-level random effects, and leave-one-out cross-validation was applied to evaluate the developed models. At the beginning of modeling, correlation analysis and best-subset regression were used to analyze the correlation between the diameter increment and the biotic and abiotic factors.

Results
(i) Sorting the categories of predictors in descending order based on the relative importance of the significant predictors, diameter growth of Dahurian larch was affected by competition, tree size, topographic conditions, stand attributes, diversity index, and climate factors, while the white birch species was affected by competition, tree size, stand attributes, climate factors, diversity index, and topographic conditions; (ii) the plot-level mixed-effects model, which achieved better fit and prediction performance than did basic linear models of individual-tree diameter growth in the cases of prediction calibration, was preferable for modeling individual-tree diameter growth; (iii) the prediction accuracy of the mixed-effects model increased gradually with increasing size of calibration sample, and the best sampling strategy was the use of nine random trees to calibrate and make predictions with the mixed-effects model for the larch and birch species; (iv) Dahurian larch was dominant in terms of interspecific competition, and the growth of this species was enhanced when it was grown with the birch.

Conclusion
In addition to biotic factors such as tree size and stand characteristics, the impact of climate on the growth of Dahurian larch and white birch should be considered in future management policies.
The cyclooxygenase-expressing mesenchyme resists intestinal epithelial injury by paracrine signaling
Authors: Wei Siting , Li Meng , Song Wanlu , Liu Jiaye , Yu Shicheng , Wang Yalong , Zhang Mengxian , Du Huijun , Liu Yuan , Liu Huidong , Fu Wei , Li Baojie , Chen Ye-Guang 
Published: August 2023

AbstractParacrine signals play pivotal roles in organ homeostasis. Mesenchymal stromal cells (MSCs) play a key role in regulating epithelium homeostasis in the intestine while their paracrine effects are poorly characterized. Here, we identified prostaglandin E2 (PGE2) secreted by cyclooxygenase (COX)-expressing MSCs as a vital factor to maintain the intestinal mucosal barrier. We found that MSCs-induced organoid swelling through paracrine effect in vitro, a process due to enhanced water adsorption and is mediated by the COX-PGE2-EP4 axis. To further explore the regulatory effect of this axis on the intestinal epithelial barrier in vivo, we established the conditional knockout mouse model to specifically delete COX in MSCs and found that PGE2 reduction downregulated the gene Muc2 and induced a gastric metaplasia-like phenotype. Moreover, PGE2 defects increased the susceptibility of intestinal epithelium to colitis. Our study uncovers the paracrine signaling of COX-expressing MSCs in intestinal mucosal barrier maintenance, providing a basis for understanding the role of mesenchymal cells in the pathophysiological function of the intestine.
Prefiltering-based reduced rank multiuser detection in uplink generalized spatial modulation massive MIMO system
Authors: Wu Wei-Chiang 
Published: August 2023

AbstractExploiting generalized spatial modulation in massive MIMO system, we develop an efficient uplink multiuser detection scheme. Different from the maximum likelihood detection algorithm, the spatial and temporal data in the proposed detection scheme are decoded in a sequential manner: At the front end of base station receiver, a bank of spatial filters is first employed to mitigate the multiuser interference using the benefits of large-scale antenna array. There then, a reduced rank generalized Eigen-value decomposition-based algorithm is proposed to identify the antennas activated at each user terminal. In the second stage, a least-squares-based multiuser detector is employed to decode the multiplexed temporal symbols. Both theoretical analysis and computer simulations are conducted to evaluate system performance in terms of correct identification probability and overall symbol error rate. Simulation results demonstrate that the proposed algorithm outperforms most of the existing algorithms yet the complexity is extensively reduced.
Channel and temporal-frequency attention UNet for monaural speech enhancement
Authors: Xu Shiyun , Zhang Zehua , Wang Mingjiang 
Published: August 2023

AbstractThe presence of noise and reverberation significantly impedes speech clarity and intelligibility. To mitigate these effects, numerous deep learning-based network models have been proposed for speech enhancement tasks aimed at improving speech quality. In this study, we propose a monaural speech enhancement model called the channel and temporal-frequency attention UNet (CTFUNet). CTFUNet takes the noisy spectrum as input and produces a complex ideal ratio mask (cIRM) as output. To improve the speech enhancement performance of CTFUNet, we employ multi-scale temporal-frequency processing to extract input speech spectrum features. We also utilize multi-conv head channel attention and residual channel attention to capture temporal-frequency and channel features. Moreover, we introduce the channel temporal-frequency skip connection to alleviate information loss between down-sampling and up-sampling. On the blind test set of the first deep noise suppression challenge, our proposed CTFUNet has better denoising performance than the champion models and the latest models. Furthermore, our model outperforms recent models such as Uformar and MTFAA in both denoising and dereverberation performance.
Causes and effects of the German energy transition in the context of environmental, societal, political, technological, and economic developments
Authors: Kappner Kai , Letmathe Peter , Weidinger Philipp 
Published: August 2023

Abstract
Background
As lignite mining protests and #FridaysForFuture demonstrations gained momentum in Germany and further protests have been developing over time, this paper investigates the various causes and effects of the country’s energy transition. Society and politics alongside economic, environmental, and technological developments have led to a profound and continuous transformation of the energy system, a transformation which is remarkable in terms of reach and speed for an economy of the size of Germany’s. Pressure to transform the country’s entire energy system even faster has recently been levelled due to the Russian invasion of Ukraine.

Results
From the perspective of the different pillars of sustainability and various stakeholder groups, this paper discusses the influences and their interdependencies towards the status quo of the German energy sector. We have used the cause-and-effect analysis method to answer the question of why major energy generators in Germany are still struggling with the energy transition, as well as the question of why a strategy towards more sustainability is needed to maintain Germany’s industrial strength in the long run. We found that energy transition in Germany is substantially driven by society, which pushes political decisions that lead to an economic transition, while environmental incidents are only triggers for further societal and political doings. Furthermore, technological developments fulfil only needs and do not necessarily hurry ahead of time.

Conclusions
Overall, the article creates a profound understanding of the factors influencing the German energy transition which is deeply embedded in the European energy system.
The progress of novel strategies on immune-based therapy in relapsed or refractory diffuse large B-cell lymphoma
Authors: Lu Tingxun , Zhang Jie , Xu-Monette Zijun Y. , Young Ken H. 
Published: August 2023

AbstractDiffuse large B-cell lymphoma (DLBCL) can be cured with standard front-line immunochemotherapy, whereas nearly 30–40% of patients experience refractory or relapse. For several decades, the standard treatment strategy for fit relapsed/refractory (R/R) DLBCL patients has been high-dose chemotherapy followed by autologous hematopoietic stem cell transplant (auto-SCT). However, the patients who failed in salvage treatment or those ineligible for subsequent auto-SCT have dismal outcomes. Several immune-based therapies have been developed, including monoclonal antibodies, antibody–drug conjugates, bispecific T-cell engaging antibodies, chimeric antigen receptor T-cells, immune checkpoint inhibitors, and novel small molecules. Meanwhile, allogeneic SCT and radiotherapy are still necessary for disease control for fit patients with certain conditions. In this review, to expand clinical treatment options, we summarize the recent progress of immune-related therapies and prospect the future indirections in patients with R/R DLBCL.
The unique gut microbiome of giant pandas involved in protein metabolism contributes to the host’s dietary adaption to bamboo
Authors: Deng Feilong , Wang Chengdong , Li Desheng , Peng Yunjuan , Deng Linhua , Zhao Yunxiang , Zhang Zhihao , Wei Ming , Wu Kai , Zhao Jiangchao , Li Ying 
Published: August 2023

Abstract
Background
The gut microbiota of the giant panda (Ailuropoda melanoleuca), a global symbol of conservation, are believed to be involved in the host’s dietary switch to a fibrous bamboo diet. However, their exact roles are still largely unknown.

Results
In this study, we first comprehensively analyzed a large number of gut metagenomes giant pandas (n = 322), including 98 pandas sequenced in this study with deep sequencing (Illumina) and third-generation sequencing (nanopore). We reconstructed 408 metagenome-assembled genomes (MAGs), and 148 of which (36.27%) were near complete. The most abundant MAG was classified as Streptococcus alactolyticus. A pairwise comparison of the metagenomes and meta-transcriptomes in 14 feces revealed genes involved in carbohydrate metabolism were lower, but those involved in protein metabolism were greater in abundance and expression in giant pandas compared to those in herbivores and omnivores. Of note, S. alactolyticus was positively correlated to the KEGG modules of essential amino-acid biosynthesis. After being isolated from pandas and gavaged to mice, S. alactolyticus significantly increased the relative abundance of essential amino acids in mice jejunum.

Conclusions
The study highlights the unique protein metabolic profiles in the giant panda’s gut microbiome. The findings suggest that S. alactolyticus is an important player in the gut microbiota that contributes to the giant panda’s dietary adaptation by more involvement in protein rather than carbohydrate metabolism.
Dissection of transcriptomic and epigenetic heterogeneity of grade 4 gliomas: implications for prognosis
Authors: Zeng Chang , Song Xiao , Zhang Zhou , Cai Qinyun , Cai Jiajun , Horbinski Craig , Hu Bo , Cheng Shi-Yuan , Zhang Wei 
Published: August 2023

Abstract
Background
Grade 4 glioma is the most aggressive and currently incurable brain tumor with a median survival of one year in adult patients. Elucidating novel transcriptomic and epigenetic contributors to the molecular heterogeneity underlying its aggressiveness may lead to improved clinical outcomes.

Methods
To identify grade 4 glioma -associated 5-hydroxymethylcytosine (5hmC) and transcriptomic features as well as their cross-talks, genome-wide 5hmC and transcriptomic profiles of tissue samples from 61 patients with grade 4 gliomas and 9 normal controls were obtained for differential and co-regulation/co-modification analyses. Prognostic models on overall survival based on transcriptomic features and the 5hmC modifications summarized over genic regions (promoters, gene bodies) and brain-derived histone marks were developed using machine learning algorithms.

Results
Despite global reduction, the majority of differential 5hmC features showed higher modification levels in grade 4 gliomas as compared to normal controls. In addition, the bi-directional correlations between 5hmC modifications over promoter regions or gene bodies and gene expression were greatly disturbed in grade 4 gliomas regardless of IDH1 mutation status. Phenotype-associated co-regulated 5hmC–5hmC modules and 5hmC–mRNA modules not only are enriched with different molecular pathways that are indicative of the pathogenesis of grade 4 gliomas, but also are of prognostic significance comparable to IDH1 mutation status. Lastly, the best-performing 5hmC model can predict patient survival at a much higher accuracy (c-index = 74%) when compared to conventional prognostic factor IDH1 (c-index = 57%), capturing the molecular characteristics of tumors that are independent of IDH1 mutation status and gene expression-based molecular subtypes.

Conclusions
The 5hmC-based prognostic model could offer a robust tool to predict survival in patients with grade 4 gliomas, potentially outperforming existing prognostic factors such as IDH1 mutations. The crosstalk between 5hmC and gene expression revealed another layer of complexity underlying the molecular heterogeneity in grade 4 gliomas, offering opportunities for identifying novel therapeutic targets.
Aging changes in maxillary anterior teeth in untreated individuals: an observational longitudinal study
Authors: Natsumeda Gabriela , Miranda Felicia , Massaro Camila , Lauris José Roberto Pereira , Garib Daniela 
Published: August 2023

Abstract
Objective
The aging of the occlusion and tooth wears influence the smile design This study aimed at evaluating the aging changes of maxillary anterior teeth in nontreated subjects.

Methods
The sample comprised dental models of 23 subjects (13 male, 10 female) with normal occlusions, taken at 13 (T1), 17 (T2) and 61 (T3) years of age. The following variables were measured in the maxillary anterior teeth using digital dental models: crown width/height proportion, anterior view width, crown angulation, gingival and incisal steps between central/lateral incisors and central incisors/canines. Interphase comparisons were evaluated using repeated measures analysis of variance followed by Tukey tests or Friedman tests. Sexual differences were evaluated using t tests (P < 0.05).

Results
From 13 to 61 years of age, a decrease of crown width/height proportion (P = 0.008 and P =  < 0.001, for the lateral incisor and canines, respectively) and mesiodistal angulation (P =  < 0.001, P = 0.001 and P = 0.025 for the central incisor, lateral incisor and canines, respectively) of the maxillary anterior teeth were observed. The steps of the gingival margin and the incisal steps decreased with aging.

Conclusions
From adolescence to late adulthood, untreated individuals with normal occlusions demonstrated changes in the maxillary anterior teeth that may impair the smile esthetics and attractiveness.
K-feldspar enrichment in the Pacific pelagic sediments before Miocene
Authors: Usui Yoichi , Yamazaki Toshitsugu 
Published: August 2023

AbstractThe mineralogy of atmospheric silicate dust controls its interaction with clouds. K-feldspar has a remarkably high ice-nucleating activity, and its distribution may have influenced the global climate throughout Earth's history. However, long-term archives of past atmospheric feldspar are not known. Here, we investigate feldspar mineralogy, content, and grain size in pelagic clay cores. Sediments around Minamitorishima Island contain > 10 wt% of K-feldspar before ~ 35 Ma, which is five times more than the younger sediments. This distribution does not resemble other volcanic minerals or geochemically estimated volcanic input, suggesting that the K-feldspars are not associated with volcanic ash. The K-feldspars are present as isolated grains as well as pseudorhombohedral microcrystals indicative of authigenic overgrowth. On the other hand, they contain some Na, arguing against a purely authigenic origin. Grain size distributions of chemically separated quartz and feldspars show stratigraphic variation analogous to other North Pacific sites, further suggesting a link to eolian materials. Sediments from a South Pacific site also show K-feldspar enrichment over plagioclase before ~ 44 Ma, although the content relative to bulk sediment does not change much. We propose that the K-feldspar may be enriched in the wide area of the Pacific before ~ 30 to 40 Ma.
Holobiont responses of mesophotic precious red coral Corallium rubrum to thermal anomalies
Authors: Tignat-Perrier Romie , van de Water Jeroen A. J. M. , Allemand Denis , Ferrier-Pagès Christine 
Published: August 2023

AbstractMarine heat waves (MHWs) have increased in frequency and intensity worldwide, causing mass mortality of benthic organisms and loss of biodiversity in shallow waters. The Mediterranean Sea is no exception, with shallow populations of habitat-forming octocorals facing the threat of local extinction. The mesophotic zone, which is less affected by MHWs, may be of ecological importance in conservation strategies for these species. However, our understanding of the response of mesophotic octocoral holobionts to changes in seawater temperature remains limited. To address this knowledge gap, we conducted a study on an iconic Mediterranean octocoral, the red coralCorallium rubrumsampled at 60 m depth and 15 °C. We exposed the colonies to temperatures they occasionally experience (18 °C) and temperatures that could occur at the end of the century if global warming continues (21 °C). We also tested their response to extremely cold and warm temperatures (12 °C and 24 °C). Our results show a high tolerance ofC. rubrumto a two-month long exposure to temperatures ranging from 12 to 21 °C as no colony showed signs of tissue loss, reduced feeding ability, stress-induced gene expression, or disruption of host-bacterial symbioses. At 24 °C, however, we measured a sharp decrease in the relative abundance of Spirochaetaceae, which are the predominant bacterial symbionts under healthy conditions, along with a relative increase in Vibrionaceae. Tissue loss and overexpression of the tumor necrosis factor receptor 1 gene were also observed after two weeks of exposure. In light of ongoing global warming, our study helps predict the consequences of MHWs on mesophotic coralligenous reefs and the biodiversity that depends on them.
The occurrence and extent of anxiety and distress among Dutch travellers after encountering an animal associated injury
Authors: Warmerdam Anouk M. T. , Luppino Floriana S. , Visser Leo G. 
Published: August 2023

Abstract
Background
Prompt administration of post-exposure prophylaxis (PEP) is crucial to prevent a fatal rabies infection after an animal associated injury (AAI), preferably within 24 h. PEP, especially in case of a type III injury for which rabies immune globulin (RIG) is needed, is difficult to obtain abroad. This, along with the fear of potentially having contracted a lethal disease, might be an important source for anxiety and distress. We investigated the occurrence and extent of self-reported anxiety and distress at different timepoints among Dutch travellers after encountering an AAI, and the involved factors.

Methods
A retrospective quantitative observational study was conducted including insured Dutch travellers who actively contacted Eurocross Assistance after encountering an AAI abroad. An online questionnaire was designed to measure anxiety and distress levels, using the HADS (Hospital Anxiety and Depression Scale) and distress thermometer at three time points: departure from home (T1), post-AAI (T2), and treatment administration (T3). Statistical analyses included T-tests, Chi-square tests, and ANCOVA analyses.

Results
We showed a significant increase in mean anxiety and distress scores at T2, and a significant decrease at T3. Women were more often anxious and distressed. Between T1 and T2, PrEP, and being aware of the risks were positively associated with anxiety levels, and PrEP and WHO region Africa with distress levels. Between T2 and T3, anxiety levels remained higher for monkey-induced injury, thoracic injuries, and WHO region Southeast Asia. PEP-delay between 24–48 h resulted in decreased distress levels at this time period, while type II injury elevated distress levels.

Conclusions
This study showed significant anxiety and distress levels after an AAI among the vast majority of travellers, which is detrimental to their health-related quality of life (HR-QOL). This highlights the importance of proper pre-travel information. In the context of rabies prevention, these results suggest that pre-travel advice and policy makers should also take aspects of HR-QOL into consideration.