bluuebunny/crossref_metadata_2025_split huggingface dataset:
SRSF3/AMOTL1 Splicing Axis Promotes the Tumorigenesis of Nasopharyngeal Carcinoma through Regulating the Nucleus Translocation of YAP1
Authors: Xu Xiao-Chen , Jiang Jiaxin , Zhou Ya-Qing , He Shuai , Liu Yang , Wei Pan-Pan , Bei Jin-Xin , Sun Jian , Luo Chun-Ling 
Published: May 2023

Abstract
Dysregulation of serine/arginine splicing factors (SRSFs) and thereby the abnormal alternative splicing (AS) has been widely implicated in the development of multiple cancers, but scarcely investigated in nasopharyngeal carcinoma (NPC). Here we examine the expression of 12 classical SRSFs between 87 NPC and 10 control samples, revealing a significant upregulation of SRSF3 and its association with worse prognosis in NPC. Functional assays demonstrate that SRSF3 exerts oncogenic function in NPC progression. Transcriptome analysis reveals 1 934 SRSF3-regulated AS events in genes involved in cell cycle and mRNA metabolism, among which generation of a long isoform of AMOTL1 (AMOTL1-L) is further verified through a direct bond of SRSF3 RRM domain with exon 12 of AMOTL1 to promote exon inclusion. Functional studies reveal that AMOTL1-L promotes proliferation and migration of NPC cells, while AMOTL1-S does not. Moreover, overexpression of AMOTL1-L significantly rescues the abovementioned inhibitory effects of SRSF3 knockdown, but not AMOTL1-S. Furthermore, compared with AMOTL1-S, AMOTL1-L has a localization preference in the intracellular than cell membrane, leading to a stronger interaction with YAP1 to promote its translocation to nucleus. Taken together, our findings identify SRSF3/AMOTL1 as a novel alternative splicing axis with pivotal roles in NPC development, which could be served as promising prognostic biomarkers and therapeutic targets of NPC.
Validation of an improved reference freeze-dried direct agglutination test for the detection of leishmaniasis in the canine reservoir
Authors: Harith Abdallah el , Abass Elfadil , Martinkovic Franjo , Mansour Durria , Osman Hussam 
Published: May 2023

Abstract
Introduction: Proper identification and management of reservoir post kala-azar dermal leishmaniasis (PKDL) and canine visceral leishmaniasis (CVL) cases are prerequisites to the effective control of visceral leishmaniasis (VL) world-wide. Unlike PKDL, CVL still awaits effective improvement because of its cryptic nature, absence of Leishmania parasite in lesions or lymph-nodes and insensitivity of tools in current use. Because of the need for certain skill and equipment, both the liquid direct agglutination test (LQ-DAT) and freeze-dried direct agglutination test (FD-DAT) versions, present in comparison with the indirect immunofluorence (IFAT) or enzyme-linked immunosorbant assay (ELISA), practical and feasible diagnostic alternatives. Due to difficulties in local production or high application cost involved, routine application of direct agglutination test (DAT) was greatly hampered in major CVL endemic areas.
Aim: Validate performance of an improved FD-DAT to suit routine and large scale application in CVL endemic areas.
Methods: Introducing of citrate-saline formaldehyde (CSF) as anti-clumping agent to replace normal saline for antigen reconstitution and drastically however eligibly lowering concentration of promastigotes (1.4X107) in comparison with the original reference FD-DAT (> 5X107/ml), To ensure optimal safety, β-mercaptoethanol (β-ME) was replaced by urea or sodium dodecyle sulphate (SDS) as a serum reducing agent.
Results: Through improving the procedure for reconstitution of FD-DAT antigen with CSF a 150% reduction in the test application cost was achieved. Expired test batches (+ 4 years earlier), were successfully revitalized to full validity. As compared to 48-hour shelf-life time for the original, an FD-DAT batch re-constituted here with CSF maintained validity for + 12 months.
Conclusion: The highly concordant results with IFAT and ELISA (One Way ANOVA Test P = 0.142, Homogeneity of Variances P = 0.009) as routine CVL diagnostics further motivate application of the improved FD-DAT for detection of the disease in endemic areas.
An Evaluation of the Cost of Human Papilloma Virus (HPV) Vaccine Delivery In Zambia
Authors: Simuyemba Moses C , Chama-Chiliba Chitalu M , Chompola Abson , Sinyangwe Aaaron , Bchir Abdallah , Asiimwe Gilbert , Masiye Felix , Chibwesha Carla 
Published: May 2023

Abstract

Background
Human papillomavirus (HPV) is a common sexually transmitted infection and the leading cause of cervical cancer. The HPV vaccine is a safe and effective way to prevent HPV infection. In Zambia, the vaccine is given during Child Health to girls aged 14 years who are in and out of school in two doses over two years. The focus of this evaluation was to establish the cost to administer a single dose of the vaccine well as for full immunisation of two doses.
Methods
For HPV costing, both top-down and micro-costing approaches were used, depending on the cost data source, and economic costs were gathered from Expanded Programme for Immunisation Costing and Financing Project (EPIC). Data was collected from eight districts in four provinces, mainly using a structured questionnaire, document reviews and key informant interviews with staff at national, district and provincial levels.
Results
Findings show that schools made up 53.3% of vaccination sites, community outreach sites 30.9% and finally health facilities 15.8%. In terms of coverage for 2020, for the eight districts sampled, schools had the highest coverage at 96.0%. Community outreach sites were at 6.0% of the coverage and health facilities accounted for only 1.0% of the coverage. School based delivery had the lowest cost economic cost at USD13.2 per dose and USD 26.4 per fully immunised child (FIC). Overall financial costs were US$6.0 per dose and US$11.9 per fully immunised child. Overall economic costs taking all delivery models into account were US$23.0 per dose and US$46.0 per FIC. The main cost drivers were human resources, building overhead and vehicles, microplanning, supplies and service delivery/outreach. were the top cost drivers. Nurses, environmental health technicians and community-based volunteers were the most involved in HPV vaccination.
Conclusions
Future planning in Zambia and other African countries conducting HPV vaccination needs to prioritise these cost drivers as well as possibly find strategies to minimise some costs. Although not a challenge now due to Gavi support, vaccine costs are a major threat to sustainability in the long run. Countries like Zambia must find strategies to mitigate against this.
One Pot Multicomponent Synthesis of Highly Substituted Imidazoles Using Tetrabutylammonium Peroxy disulfate as a Catalyst
Authors: Nukala Ramesh , Gullapelli Kumaraswamy , Konakanchi Ramaiah 
Published: May 2023

Abstract
Tetra butyl ammonium Peroxy disulfate has been found to be an efficient and rapid medium for thesynthesis of novel substituted imidazoles. The current investigation involving different mole ratio of catalyst and different solvents were used. The employed strategy/methodology is efficient, economic and sustainable owing to its distinctive characteristics such as eco-friendly low reaction times, excellent yields, cost-effective due to avoid the use of toxic solvents and reusability of the catalyst, simple workup procedure, and extensive applicability. Results reveal that the 20% mole ratio and acetonitrile gave excellent yield. Structures of newly synthesized compounds have been confirmed by Proton NMR, 13CNMR, IR and mass spectra.
Association between cardiovascular health metrics and restless legs syndrome: A population-based study
Authors: Wang Dongxiao , Lin Jianping , Li Qingxia , Ma Xiuxia , Zhang Xuetong , Luo Shan , Liao Renfeng 
Published: May 2023

Abstract
Background Cardiovascular diseases is increasingly identified to be related to the restless legs syndrome (RLS). However, the relationship between Cardiovascular Health Metric (CVH) and RLS need to be further confirmed. The present study aimed to assess the association of overall CVH metric and 7 Simple’s Life (LS7) with the RLS risk.
Methods In a cross-sectional population-based study, 3,772 adults (57.6 ± 5.11 years of age) were recruited and completed the structured questionnaire between January 2 and May 21, 2022. Blood sample and other body measurements were obtained by trained nurses. The definition and score of CVH metric was determined by the attendance of LS7, and the RLS was diagnosed by the International Restless Legs Syndrome Study Group (IRLSSG) criteria. Multivariate logistic regression models were applied to examine the associations of overall CVH metric and its SL7 profiles with prevalence of RLS.
Results Overall, 301 (7.98%) were diagnosed with RLS. Multivariable logistic regression analysis showed that, in comparison to inadequate scores, higher scores of overall CVH metric and body mass index (BMI), physical activity, blood pressure and total cholesterol (TC) metric were negatively associated with the prevalence of RLS (multi-adjusted odd ratios [ORs] ranged from 0.32 to 0.67, all P for trend < 0.05). Per 1-SD increase in over CVH metric and each SL7 profile yielded the similar results (ORs ranged from 0.65 to 0.85, all P for trend < 0.05). Significant differences in the association of RLS with smoke profile and overall CVH metric were detected with females (P for interaction = 0.005) and older participants (P for interaction = 0.013), respectively.
Conclusion To be at an ideal behavioural CVH may be benefit in RLS, especially for women and older people. Interventions concerning to promote and preserve favourable CVH should be regarded in the prevention and treatment of RLS.
The effect of ultrasonic rolling on the fatigue performance of laser-welded TC4 titanium alloy joints
Authors: Jiahui Cong , Jiayuan Gao , Song Zhou , Zhichao Zhang , Jiahao Wang , Naijing Wang 
Published: May 2023

Abstract

In order to improve the fatigue performance of TC4 titanium alloy laser welded joints, ultrasonic rolling treatment was adopted in this study, and different passes of rolling process were used. The research results show that the ultrasonic rolling treatment significantly improves the fatigue limit and fatigue life of the weldment. At room temperature, the fatigue strength of the weldment increases by 2.04–4.58%, and the corrosion fatigue life increases by 1.71 to 3.05 times. This study also deeply analyzed the significant effects of ultrasonic rolling treatment on surface morphology, microstructure, surface residual stress and microhardness to reveal its strengthening mechanism. It was found that the ultrasonic rolling treatment shifted the crack initiation point to the subsurface and formed a hard layer with high residual stress on the surface by applying significant static pressure input and multiple treatments. This change makes the fatigue stripes narrower and denser. Compared with the traditional weld surface treatment method, ultrasonic rolling treatment significantly improves the surface quality and fatigue performance of TC4 titanium alloy laser welded joints.
Evaluating knowledge, Attitudes, and Practices Regarding Interruption of Enteral Nutrition in ICU Medical staff:A descriptive cross-sectional survey
Authors: Yuanyuan Mi , fei Tian , Chao Zeng , Liu Jing , Haiyan Huang 
Published: May 2023

Abstract
Objectives: Investigate the status of knowledge, attitude, and practice(KAPs) on Interruption of enteral feeding among ICU medical staff, and explore influencing factors of KAPs score.
Design: questionnaires including general information as well as knowledge, attitude, and practice(KAPs) on Interruption of enteral feeding were distributed in November 2021 to 575 ICU medical staff. t-test and logistic and linear regressions were used to assess Status of interruption of enteral feeding and relevant influencing factors.
Setting: Hospitals in Hubei Province(n =14).
Participants: A total of 575 ICU medical staff including: nurses(n =401); doctors(n =153); all staff responded and 554 were included in the analysis.
Results: of the 554 participating ICU medical staff, the median score in knowledge, attitude and practice was 55(47, 60), 50(44, 50) ,73(67, 80),respectively. Having an advanced degree((bachelor's degree or above,p=0.007), the high grade hospital(Class III A, p =0.020), undertaking a more comprehensive training(Learned but not comprehensive,p=0.037;systematic learning, p <0.001), Main learning approaches(Books and Internet; p =0.029), the frequency in academic journals(often, p =0.005) was the contributing factor to score of total KAPs; undertaking a comprehensive the training(Systematic learning, p <0.001), High frequency in Academic journals(sometimes, p=0.008; often, p<0.001; always, p =0.032) was the contributing factor to score of knowledge dimension; Having an advanced degree(bachelor's degree or above, p =0.004) was the socre of attitude dimension; Having an Advanced degree(bachelor's degree or above, p =0.026) and undertaking a comprehensive the training(systematic learning, p =0.013) was the contributing factor to score of parctice dimension; There was a significant positive correlation between the total score and score of knowledge, attitude and practice (p <0.05).
Conclusions: Based on the findings, the hospital management department should formulate targeted training programs to further improve the level of enteral nutrition interruption among ICU medical staff.
Bacterial Isolates and Characteristics of Children With Febrile Neutropenia on Treatment for Cancer at Moi Teaching and Referral Hospital, Kenya.
Authors: Kipchumba Samuel , Njuguna Festus M. , Nyandiko Winstone M. 
Published: May 2023

Abstract
Purpose
This study aimed at identifying the patient characteristics of children with febrile neutropenia, the associated bacterial organisms, and their sensitivity patterns.
Methods
A descriptive cross-sectional study was carried out at the MTRH, paediatric oncology ward, from June 2021 to April 2022. A total of 110 children who developed fever and neutropenia while on chemotherapy were enrolled. Blood samples for culture were drawn aseptically. Patient characteristics were presented in frequency tables. Antimicrobial sensitivity patterns were plotted in tables against the bacterial isolates cultured. Chi-square/Fischer’s exact test were used to determine any association between patient characteristics, bacterial growth and antimicrobial sensitivity.
Results
Majority 66 (60%) were males. The median age was 6.3 (SD 3.7) years. Majority of the patients 71 (64.5%) had haematological malignancies, the most common being Acute Myeloid Leukaemia (AML). There was a significant association between severity of neutropenia and haematological malignancies p = 0.028. In total, 31/110 (28.2%) blood cultures were positive for bacterial growth. Gram-positive bacteria were more frequent at 20 (58.1%). The most common organism was Escherichia coli 6 (18.2%), followed by Staphylococcus aureus at 5 (15.2%.) All the isolates were sensitive to linezolid and vancomycin and also showed good sensitivity towards meropenem at 10/11 (90.9%). High resistance to cephalosporins was noted with ceftriaxone at 5/6 (83.3%), cefepime at 4/7 (57.1%) and ceftazidime at 3/4 (75%).
Conclusion
The most common malignancy associated with febrile neutropenia was AML. Gram-positive bacteria were the most common isolates. There was high resistance towards cephalosporins but all were sensitive to linezolid and vancomycin.
Multi-directional neutron dark-field imaging with single absorption grating
Authors: Busi Matteo , Shen Jiazhou , Bacak Michael , Zdora Marie , Valsecchi Jacopo , Strobl Markus 
Published: May 2023

Abstract
Neutron dark-field imaging is a powerful technique for investigating the microstructural properties of materials through high-resolution full-field mapping of small-angle scattering. However, conventional neutron dark-field imaging utilizing Talbot-Lau interferometers is limited to probing only one scattering direction at a time. Here, we introduce a novel multi-directional neutron dark-field imaging approach that utilizes a single absorption grating with a two-dimensional pattern to simultaneously probe multiple scattering directions. The method is demonstrated to successfully resolve fiber orientations in a carbon compound material as well as the complex morphology of the transformed martensitic phase in additively manufactured stainless steel dogbone samples after mechanical deformation. The latter results reveal a preferential alignment of transformed domains parallel to the load direction, which is verified by EBSD. The measured real-space correlation functions are in good agreement with those extracted from the EBSD map. Our results demonstrate that multi-directional neutron dark-field imaging is overcoming significant limitations of conventional neutron dark-field imaging in assessing complex heterogeneous anisotropic microstructures and providing quantitative structural information on multiple length scales.
mm-Wave Radar-Based Multiple Patients’ Breathing and Heart Rates Measurement
Authors: Ahmed Sajid , Abdullah Muhammad , Alouini Mohamed-Slim 
Published: May 2023

Abstract
This paper demonstrates an mm-wave frequency-modulated continuous-wave (FMCW) radar to simultaneously measure multiple stationary patients’ breathing and heart rates. The proposed algorithm cancels the background interference and applies fast-Fourier-transform on fast-time samples to measure the location and on slow-time samples to measure the breathing and heart rates. The algorithm is implemented on a commercially available Infineon’s "Position2Go" module, which is one transmit and two receive antenna FMCW radar and operates at 24GHz. In contrast to the reported systems, our inexpensive system can reliably measure multiple patients’ vital signs and localize them in range. The proposed system can find applications in the healthcare system by offering a centralized inexpensive wireless vital sign monitoring of multiple patients without fixing any probe on the patient’s body.
Transparent, Mechanically Robust, Low-temperature- tolerance, and Stretchable Ionogels Enhanced by Konjac Glucomannan toward Wireless Strain Sensors
Authors: Ye Zhifan , Zheng Yijia , Jia Qihan , Wang Haibo , Xiong Junjie , Wang Shuang 
Published: May 2023

Abstract
Electronic skins (E-skins) are capable of detecting human health and movement, which has promising potential in the fields of human-machine interactions and artificial intelligence. However, traditional hydrogels-based E-skins suffer from poor mechanical strength, low conductivity, and instability owing to the evaporation of water. Herein, to advance the unsatisfaction of E-skins above, a semi-interpenetrating network developed by polysaccharide biomass konjac glucomannan (KGM) was introduced into covalent-crosslinked network (PAA-co-PAM) to synthesize a transparent, tough, nonvolatile, and highly stretchable ionogel with an ionic liquid (EMIM:DCA) as conductive media. This ionogel exhibited extraordinary mechanical strength (tensile strength 2.77 MPa), outstanding mechanically durability (100 stretching cycles of 250%) and elongation (elongation at break 997%). More importantly, the ionogel demonstrated remarkable anti-freezing performance (high flexibility at -20℃) and high conductivity (3.94 mS/cm) in the absence of water. Besides, after assembly from konjac glucomannan-enhanced ionogel, the sensor exhibited comprehensive strain sensing performance, which could effectively and accurately monitor the human motion via Bluetooth transmission. This strategy paves the way for a viable new generation of multifunctional biomimetic super-sensitive sensors, which have great promise for applications such as intelligent devices, health detection, and biomedical monitoring in harsh conditions.
WITHDRAWN: Assessment of statistical and machine learning methods for landslide susceptibility of Pithoragarh, Uttarakhand (India)
Authors: Bhardwaj Vanshika , Singh Kanwarpreet 
Published: May 2023

Abstract
Landslides are the most prevalent natural hazard in hilly regions of India. These can have a significant impact on the economy of a nation. This study examines the landslide susceptibility of the Pithoragarh, Uttarakhand, India, utilising various susceptibility methods, such as Frequency Ratio (FR), Information Value (IV), Weight of Evidence (WOE), and Certainty Factor (CF). The LSZ modelling was performed using fourteen landslide causative factors. Based on past landslide data, landslide locations were identified, which were further divided into a 70/30 ratio, with 70 representing training and 30 representing validation. Validation of the findings of the predicted maps of landslide susceptibility using Area under Curve (AUC) indicates that the predicted map using the FR approach has the highest prediction rate compared to other methods used for landslide susceptibility prediction. Also to check the feasibility of the machine learning method, we have considered logistic regression (LR) analysis using five out of fourteen factors. AUC revealed that LR has higher accuracy than Analytical Hierarchy Process (AHP) and Shannon Entropy (SE). Also, validation of all the models was done using Landslide Density Index (LDI) which shows the validity of all models. Thus, the results of all models can be used to predict landslide susceptibility in Pithoragarh.
Lump, periodic solution in separable form, and periodic-soliton solutions for the (2+1)-D Calogero–Bogoyavlenskii–Schiff equation
Authors: Khan Mahbub Hassan , Islam Shariful , ALi Ahmed Refaie 
Published: May 2023

Abstract
Under examination in this manuscript is a (2+1)-D generalized Calogero–Bogoyavlenskii–Schiff equation is considered through a criterion variable transition in which a dominating variable involved. Based on the Hirota bilinear method, we build novel structures entirely innovative lump solutions, periodic solutions in separable form, and periodic-soliton solutions and also perforated appearance of two-solitary wave are obtained. Furthermore, we demonstrate that the constraints that lump solutions meet are through to satisfy a number of significant features, such as navigation, polarity and nonlinear analysis. With the aid of Maple, the 3-D plot and contour plot, the physical properties of these vibrations are very effectively explained. The obtained results can improve the dynamics of higher-dimensional nonlinear water wave’s scenarios in fluids and plasma phenomena.