bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge fields and emerging trends about extracellular matrix in carotid artery disease from 1990 to 2021: analysis of the scientific literature
Authors: Xu Ran , Li Tianhua , Li Zhiqing , Kong Wei , Wang Tao , Zhang Xiao , Luo Jichang , Li Wenjing , Jiao Liqun 
Published: August 2023

Abstract
Background
Stroke is a heavy burden in modern society, and carotid artery disease is a major cause. The role of the extracellular matrix (ECM) in the development and progression of carotid artery disease has become a popular research focus. However, there is no published bibliometric analysis to derive the main publication features and trends in this scientific area. We aim to conduct a bibliometric analysis to reveal current status of ECM in carotid artery disease and to predict future hot spots.

Methods
We searched and downloaded articles from the Web of Science Core Collection with “Carotid” and “Extracellular Matrix” as subject words from 1990 to 2021. The complete bibliographic data were analyzed by Bibliometrics, BICOMB, gCLUTO and CiteSpace softwares.

Results
Since 1990, the United States has been the leader in the number of publications in the field of ECM in carotid artery disease, followed by China, Japan and Germany. Among institutions, Institut National De La Sante Et De La Recherche Medicale Inserm, University of Washington Seattle and Harvard University are in the top 3. “Arteriosclerosis Thrombosis and Vascular Biology” is the most popular journal and “Circulation” is the most cited journal. “Clowes AW”, “Hedin Ulf” and “Nilsson Jan” are the top three authors of published articles. Finally, we investigated the frontiers through the strongest citation bursts, conducted keyword biclustering analysis, and discovered five clusters of research hotspots. Our research provided a comprehensive analysis of the fundamental data, knowledge organization, and dynamic evolution of research about ECM in carotid artery disease.

Conclusions
The field of ECM in carotid artery disease has received increasing attention. We summarized the history of the field and predicted five future hotspots through bibliometric analysis. This study provided a reference for researchers in this fields, and the methodology can be extended to other fields.
Modeling genotype × environment × management interactions for a sustainable intensification under rainfed wheat cropping system in Morocco
Authors: Mamassi Achraf , Balaghi Riad , Devkota Krishna Prasad , Bouras Hamza , El-Gharous Mohamed , Tychon Bernard 
Published: August 2023

AbstractUnder the conditions of Moroccan rainfed agricultural areas, wheat cropping systems—the population’s basic staple food—are subject to a set of limitations that seasonally impact crop production and farmers’ incomes, thus national food security. In the last decades, the major constraints were often related to the country’s Mediterranean-type climate, through the intense recurrence of drought events and high inter- and intra-annual rainfall fluctuations. Similarly, various forms of soil degradation inhibit the potential of this slowly renewable resource to support wheat crop intensification and ensure livelihoods. However, the limitations sometimes surpass the environmental factors to implicate the inappropriate crop management strategies applied by farmers. In Moroccan rainfed areas, production problems linked to crop management practices result principally from a shortage in the provision of knowledge to Moroccan small farmers, or their indigent economic situation that limits farmers’ capacity to adopt, qualitatively and quantitatively, efficient strategies. Advanced technologies (remote sensing or crop modeling) play key roles in assessing wheat cropping systems in Moroccan rainfed areas. Due to the difficulties of using conventional experience-based agronomic research to understand Genotype × Environment × Management (G × E × M) interactions, the substantial benefits of crop modeling approaches present a better alternative to provide insights. They allow the provision of simpler, rapid, less expensive, deep, and potentially more accurate predictive knowledge and understanding of the status of cropping systems. In the present study, we highlight the constraints that surround wheat cropping systems in Moroccan rainfed conditions. We emphasize the efficiency of applying crop modelling to analyze and improve wheat cropping systems through three main themes: (i) preserving food security, (ii) supporting general adaptation strategies to face climate change effects and extreme events, and (iii) recommending within-season and on-farm crop management advice. Under Moroccan context, crop modeling works have mainly contributed to increase understanding and address the climate change effects on wheat productivity. Likewise, these modeling efforts have played a crucial role in assessing crop management strategies and providing recommendations for general agricultural adaptations specific to Moroccan rainfed wheat.
Determining the utility of diagnostic genomics: a conceptual framework
Authors: Mallett Andrew , Stark Zornitza , Fehlberg Zoe , Best Stephanie , Goranitis Ilias 
Published: August 2023

Abstract
Background
Diagnostic efficacy is now well established for diagnostic genomic testing in rare disease. Assessment of overall utility is emerging as a key next step, however ambiguity in the conceptualisation and measurement of utility has impeded its assessment in a comprehensive manner. We propose a conceptual framework to approach determining the broader utility of diagnostic genomics encompassing patients, families, clinicians, health services and health systems to assist future evidence generation and funding decisions.

Body
Building upon previous work, our framework posits that utility of diagnostic genomics consists of three dimensions: the domain or type and extent of utility (what), the relationship and perspective of utility (who), and the time horizon of utility (when). Across the description, assessment, and summation of these three proposed dimensions of utility, one could potentially triangulate a singular point of utility axes of type, relationship, and time. Collectively, the multiple different points of individual utility might be inferred to relate to a concept of aggregate utility.

Conclusion
This ontological framework requires retrospective and prospective application to enable refinement and validation. Moving forward our framework, and others which have preceded it, promote a better characterisation and description of genomic utility to inform decision-making and optimise the benefits of genomic diagnostic testing.
Examining psychometric properties of the Iranian version of exclusive breastfeeding social support scale (EBFSS)
Authors: Mashayekh-Amiri Sepideh , Hosseinzadeh Mina , Jafarabadi Mohammad Asghari , Soltani Sepideh , Mirghafourvand Mojgan 
Published: August 2023

Abstract
Background
The exclusive breastfeeding (EBF) is undeniably proven significant in mothers’ health and infants’ growth and survival. Its persistence has many familial, social, and economical benefits. Social support is known to be an effective factor in EBF’s success and sustainability. However, Exclusive breastfeeding social support (EBFSS) scale validity and reliability is not evaluated in Iran. This study aimed to determine the psychometric properties of EBFSS during postpartum period in Tabriz city, Iran.

Methods
It is a cross-sectional study with descriptive survey method performed between March 2021 and August 2022. Psychometric properties were determined for the Persian version of EBFSS in six stages: translation process, evaluating content validity, face validity, construct validity, discriminant validity, and reliability. A group of experts (n = 10), followed by a group of women with EBF (n = 10), evaluated the instrument’s items based on content and face validities, respectively. A cross-sectional study using the multi-stage cluster random sampling method on 348 women with EBF in the first four months after delivery was conducted to determine the construct validity. The internal consistency and repeatability (test-retest on 30 women, 2 weeks apart) were used to find out the reliability.

Results
Content validity ratio (CVR), content validity index (CVI), and impact score were 0.98, 0.98, and 3.54 for EBFSS, respectively. This indicates a good content and face validity. Exploratory factor analysis (EFA) was performed on 16 items to examine the construct validity identified emotional, instrumental, and informational factors. These factors explained 59.26% of the cumulative variance. The fit indices (CFI = 0.98، TLI = 0.95، χ2/df = 4.20، RMSEA = 0.07 and SRMSEA = 0.05) confirmed the validity of the model in a confirmatory factor analysis (CFA). The internal consistency was examined through Cronbach’s alpha and McDonald’s omega coefficients that were 0.90 and 0.92, respectively. Finally, Repeatability and reproducibility were found 0.97 (95% CI: 0.92 to 0.99) using Intra-class correlation. This shows an appropriate reliability of the instrument.

Conclusions
The research findings indicate that the Persian version of the EBFSS has appropriate psychometric properties for evaluating the social support in Iranian women with EBF. This means healthcare providers can use it for screening social support in EBF. Researchers also can use it as a valid instrument.
Effect of educational program on the psychological challenges of electronic learning among university nursing students: a quasi-experimental study
Authors: Mohammed Bakheeta AbdEl-Aziz , Mohammed Ikram Ibraheem , Ahmed Hossam Khalifa , Abd El-Naser Amera Azzet 
Published: August 2023

AbstractThe COVID-19 pandemic has precipitated a paradigm shift in educational methodologies worldwide, necessitating a swift transition to e-learning platforms. Consequently, nursing students pursuing university degrees have encountered unique obstacles as they adapt to the e-learning milieu. This study aimed to evaluate the impact of an educational program on e-learning challenges for enhancing the psychological well-being of university nursing students. A quasi-experimental approach utilizing pretest–posttest evaluations was employed to appraise the program’s effects on diverse psychological well-being variables. In the initial phase, an extensive literature review was conducted to identify pertinent challenges encountered by nursing students regarding e-learning. Based on this review, an educational program was formulated to address these challenges and fostering psychological well-being. The participants consisted of 341 students who were selected from the Faculty of Nursing at Assiut University. To evaluate the impact of the program, multiple psychological well-being variables, such as anxiety levels, motivation, and satisfaction with the e-learning experience, were measured both before and after the intervention. Psychological challenges towards electronic learning questionnaires were utilized as data collection tools. This study endeavors to provide valuable insights into the effectiveness of the educational program in enhancing the psychological well-being of university nursing students. Here we show that by addressing these challenges and promoting psychological well-being, the educational program has the potential to contribute to the overall success and satisfaction of nursing students in their academic pursuits. Subsequent investigations should delve into the enduring repercussions of such programs and assess their efficacy in diverse academic fields.
The impact of intraoperative intravenous lidocaine infusion on early postoperative pain after complex spine surgeries
Authors: Hassan Eslam Reda , Fawaz Ahmed Ali , Hefny Sameh Salem , Abdelrahman Tamer Nabil 
Published: August 2023

Abstract
Background
This study aimed to assess the effect of intravenous lidocaine infusion affected on early postoperative pain control after complex spin surgeries.
Ninety patients who were scheduled for complex spine surgery were included in this prospective double-blinded controlled trial. They were randomly assigned to one of two groups: L and P. Patients in group L received a loading dose of lidocaine 1 mg/kg then followed by 1.5 mg/kg/h infusion till the end of the surgery, while in group P, lidocaine was replaced with normal saline.

Results
The pain score assessed by VAS at rest showed statistically significant lower values in group L at 30 min, 8, 12, and 24 h postoperatively. VAS during movement was significantly higher in group P only after 24 h postoperatively. The entire dose of intraoperative fentanyl consumed was significantly lower in group L. The time elapsed to ask for the first dose of rescue analgesia was significantly longer in group L. The first rescue dose of nalbuphine was significantly lower in group L. In group P, the overall dose of narcotics consumed in the first postsurgical day was significantly higher.

Conclusions
When compared to placebo, lidocaine infusion significantly reduced the postoperative pain scores, as well as the entire dose of intraoperative and postoperative narcotics used.
Effect of Testosterone Replacement Therapy on Sexual Function and Hypogonadal Symptoms in Men with Hypogonadism
Authors: Pencina Karol M , Travison Thomas G , Cunningham Glenn R , Lincoff A Michael , Nissen Steven E , Khera Mohit , Miller Michael G , Flevaris Panagiotis , Li Xue , Wannemuehler Kathleen , Bhasin Shalender 
Published: August 2023

Abstract

Context
Few long-term randomized trials have evaluated the efficacy of testosterone replacement therapy (TRT) in improving sexual function and hypogonadal symptoms in men with hypogonadism and whether effects are sustained beyond 12 months.

Objective
The Testosterone Replacement therapy for Assessment of long-term Vascular Events and efficacy ResponSE in hypogonadal men (TRAVERSE) study evaluated the effect of TRT on major adverse cardiovascular events in middle-aged and older men with hypogonadism. The Sexual Function Study, nested within the parent trial, determined testosterone's efficacy in improving sexual activity, hypogonadal symptoms, libido, and erectile function among men reporting low libido.

Methods
Among 5204 men, 45-80 years, with 2 testosterone concentrations &lt;300 ng/dL, hypogonadal symptoms, and cardiovascular disease (CVD) or increased CVD risk enrolled in the TRAVERSE trial, 1161 with low libido were enrolled in the Sexual Function Study (587 randomized to receive 1.62% testosterone gel and 574 to placebo gel for the duration of their participation in the study). Primary outcome was change from baseline in sexual activity score. Secondary outcomes included hypogonadal symptoms, erectile function, and sexual desire.

Results
TRT was associated with significantly greater improvement in sexual activity than placebo (estimated mean [95% CI] between-group difference 0.49 [0.19,0.79] and 0.47 [0.11, 0.83] acts per day at 6 and 12 months, respectively; omnibus test P = .011); treatment effect was maintained at 24 months. TRT improved hypogonadal symptoms and sexual desire, but not erectile function, compared with placebo.

Conclusion
In middle-aged and older men with hypogonadism and low libido, TRT for 2 years improved sexual activity, hypogonadal symptoms, and sexual desire, but not erectile function.
Luteal Lipid Droplets: A Novel Platform for Steroid Synthesis
Authors: Plewes Michele R , Talbott Heather A , Saviola Anthony J , Woods Nicholas T , Schott Micah B , Davis John S 
Published: August 2023

Abstract
Progesterone is an essential steroid hormone that is required to initiate and maintain pregnancy in mammals and serves as a metabolic intermediate in the synthesis of endogenously produced steroids, including sex hormones and corticosteroids. Steroidogenic luteal cells of the corpus luteum have the tremendous capacity to synthesize progesterone. These specialized cells are highly enriched with lipid droplets that store lipid substrate, which can be used for the synthesis of steroids. We recently reported that hormone-stimulated progesterone synthesis by luteal cells requires protein kinase A–dependent mobilization of cholesterol substrate from lipid droplets to mitochondria. We hypothesize that luteal lipid droplets are enriched with steroidogenic enzymes and facilitate the synthesis of steroids in the corpus luteum. In the present study, we analyzed the lipid droplet proteome, conducted the first proteomic analysis of lipid droplets under acute cyclic adenosine monophosphate (cAMP)-stimulated conditions, and determined how specific lipid droplet proteins affect steroidogenesis. Steroidogenic enzymes, cytochrome P450 family 11 subfamily A member 1 and 3 beta-hydroxysteroid dehydrogenase (HSD3B), were highly abundant on lipid droplets of the bovine corpus luteum. High-resolution confocal microscopy confirmed the presence of active HSD3B on the surface of luteal lipid droplets. We report that luteal lipid droplets have the capacity to synthesize progesterone from pregnenolone. Lastly, we analyzed the lipid droplet proteome following acute stimulation with cAMP analog, 8-Br-cAMP, and report increased association of HSD3B with luteal lipid droplets following stimulation. These findings provide novel insights into the role of luteal lipid droplets in steroid synthesis.
Allostasis in Neuroendocrine Systems Controlling Reproduction
Authors: Carrasco Rodrigo A , Breen Kellie M 
Published: August 2023

Abstract
Allostasis provides a supporting role to the homeostatic control of biological variables in mammalian species. While the concept of homeostasis is related to the control of variables within a set point or range that are essential to life, allostasis refers to systems that facilitate adaptation to challenges that the organism faces and the new requirements for survival. Essential for such adaptation is the role played by the brain in eliciting neural and neuroendocrine responses. Reproductive function is fundamental for the survival of species but is costly in energetic terms and requires a synchrony with an ever-changing environment. Thus, in many species reproductive function is blocked or delayed over immediate challenges. This review will cover the physiological systems and neuroendocrine pathways that supply allostatic control over reproductive neuroendocrine systems. Light, hypoxia, temperature, nutrition, psychosocial, and immune mediators influence the neuroendocrine control of reproductive functions through pathways that are confluent at the paraventricular nucleus; however, understanding of the integrative responses to these stimuli has not been clarified. Likely, the ultimate consequence of these allostatic mechanisms is the modification of kisspeptin and gonadotropin-releasing hormone neuronal activity, thus compromising reproduction function in the short term, while preserving species survivability.
Human in vivo medial gastrocnemius gear during active and passive muscle lengthening: effect of inconsistent methods and nomenclature on data interpretation
Authors: Pinto Matheus Daros , Nosaka Kazunori , Wakeling James M. , Blazevich Anthony J. 
Published: August 2023

ABSTRACT
‘Muscle gear’ is calculated as the ratio of fascicle-to-muscle length change, strain, or velocity. Inconsistencies in nomenclature and definitions of gear exist across disciplines partly due to differences in fascicle [curved (Lf) versus linear (Lf,straight)] and muscle [whole-muscle belly (Lb) versus belly segment (Lb,segment)] length calculation methods. We tested whether these differences affect gear magnitude during passive and active muscle lengthening of human medial gastrocnemius of young men (n=13, 26.3±5.0 years) using an isokinetic dynamometer. Lb, Lb,segment, Lf and Lf,straight were measured from motion analysis and ultrasound imaging data. Downshifts in belly gear but not belly segment gear occurred with muscle lengthening only during active lengthening. Muscle gear was unaffected by fascicle length measurement method (P=0.18) but differed when calculated as changes in Lb or Lb,segment (P&lt;0.01) in a length-dependent manner. Caution is therefore advised for the use and interpretation of different muscle gear calculation methods and nomenclatures in animal and human comparative physiology.
CRISPR-based knockout and base editing confirm the role of MYRF in heart development and congenital heart disease
Authors: Doering Lino , Cornean Alex , Thumberger Thomas , Benjaminsen Joergen , Wittbrodt Beate , Kellner Tanja , Hammouda Omar T. , Gorenflo Matthias , Wittbrodt Joachim , Gierten Jakob 
Published: August 2023

ABSTRACT
High-throughput DNA sequencing studies increasingly associate DNA variants with congenital heart disease (CHD). However, functional modeling is a crucial prerequisite for translating genomic data into clinical care. We used CRISPR-Cas9-mediated targeting of 12 candidate genes in the vertebrate model medaka (Oryzias latipes), five of which displayed a novel cardiovascular phenotype spectrum in F0 (crispants): mapre2, smg7, cdc42bpab, ankrd11 and myrf, encoding a transcription factor recently linked to cardiac-urogenital syndrome. Our myrf mutant line showed particularly prominent embryonic cardiac defects recapitulating phenotypes of pediatric patients, including hypoplastic ventricle. Mimicking human mutations, we edited three sites to generate specific myrf single-nucleotide variants via cytosine and adenine base editors. The Glu749Lys missense mutation in the conserved intramolecular chaperon autocleavage domain fully recapitulated the characteristic myrf mutant phenotype with high penetrance, underlining the crucial function of this protein domain. The efficiency and scalability of base editing to model specific point mutations accelerate gene validation studies and the generation of human-relevant disease models.
An examination of the Petroleum Industry Act 2021: prospects, challenges, and the way forward
Authors: Borha David Oladeji Ehijie , Olujobi Olusola Joshua 
Published: August 2023

Background: The study examines the gaps in the provisions of the Petroleum Industry Act (PIA) that could hinder the effective application of the Act in attaining its objectives. The repealed Petroleum Act of 1969 became obsolete and largely incapable of meeting the emerging global best practices in the industry due to inadequate sanctions, failure to address the aspirations of the people of the oil-bearing states, among others. Hence, the need for the PIA of 2021 to overhaul the industry to meet global standards though some controversial provisions that pose challenges to its proper implementation. Methods: The study examines the PIA to identify its prospects, challenges, and the way forward. The methodology the study utilises is doctrinal research with reports from existing literature and tertiary data sources such as newspapers, the Internet, and websites. Pertinent data collected from these sources were theoretically analysed and argued with current literature on the subject. Results: The finding is that the PIA does not make adequate provisions for the energy transition in line with Nigeria’s Nationally Determined Contributions (NDC) under the Paris agreement; the PIA was provided for weak institutions, which translates to weak implementation and enforcement of the law which further widening the gap between the law and reality. Conclusions: The study concluded that, although the Act delivered the much-needed stability in the petroleum industry in Nigeria, there is a need for an overhaul of the Act to further protect the interest of host communities and allow for co-ownership of petroleum resources by the state government.