bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemoautotrophic sulphur oxidizers dominate microbial necromass carbon formation in coastal blue carbon ecosystems
Authors: Yu Xiaoli , Qian Lu , Tu Qichao , Peng Yisheng , Wang Cheng , Wu Daoming , He Ziying , Shu Longfei , He Qiang , Tian Yun , Yin Kedong , Wang Shanquan , Yan Qingyun , Zhong Qiuping , He Zhili 
Published: August 2023

Abstract

Coastal blue carbon (C) ecosystems are recognized as efficient natural C sinks and play key roles in mitigating global climate change. Microbially driven C, nitrogen (N) and sulphur (S) cycles are crucial for ecosystem functioning, but how microorganisms drive C sink formation and C sequestration in coastal sediments remains unclear.

In this study, we conducted a comprehensive analysis of amino sugars, C, N and S cycling genes/pathways and their associated taxa in coastal sediments of native (Cyperus malaccensis and Kandelia obovata) and alien (Spartina alterniflora and Sonneratia apetala) vegetation.

Compared to the alien‐vegetated coastal sediment, the native‐vegetated coastal sediment had significantly (p < 0.05) higher microbial necromass C and higher functional potentials of chemoautotrophic C fixation, C degradation, methane cycling, N2 fixation, S oxidation and sulphate reduction. Also, our analysis of coastal sediment microbiomes showed that S oxidation could be coupled with C fixation and/or nitrate/nitrite reduction. S oxidation, C degradation and C fixation were found to be key functional pathways for predicting sediment microbial necromass C. Additionally, the sulphur‐oxidizing Burkholderiales metagenome‐assembled genomes (MAGs) were a key functional group that dominated chemoautotrophic C fixation in coastal sediments.

These results suggested that chemoautotrophic S oxidizers, in particular Burkholderiales with a novel lineage, might be the key microbial group that dominates microbial necromass C formation in coastal sediments through microbial anabolism (C fixation);the coupling of microbially driven C, N and S cycling processes; and the deposition of microbially derived C. This study provides novel insights into the importance of chemoautotrophic S oxidizers for microbial necromass formation and shed new light on the microbial mechanism of C sink formation in coastal ecosystems, which also has important implications for enhancing C sequestration in coastal wetlands.

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Pseudomonas fluorescensMFE01 delivers a putative type VI secretion amidase that confers biocontrol against the soft‐rot pathogen Pectobacterium atrosepticum
Authors: Bourigault Yvann , Dupont Charly A. , Desjardins Jonas B. , Doan Thierry , Bouteiller Mathilde , Le Guenno Hugo , Chevalier Sylvie , Barbey Corinne , Latour Xavier , Cascales Eric , Merieau Annabelle 
Published: August 2023

AbstractThe type VI secretion system (T6SS) is a contractile nanomachine widespread in Gram‐negative bacteria. The T6SS injects effectors into target cells including eukaryotic hosts and competitor microbial cells and thus participates in pathogenesis and intermicrobial competition. Pseudomonas fluorescens MFE01 possesses a single T6SS gene cluster that confers biocontrol properties by protecting potato tubers against the phytopathogen Pectobacterium atrosepticum (Pca). Here, we demonstrate that a functional T6SS is essential to protect potato tuber by reducing the pectobacteria population. Fluorescence microscopy experiments showed that MFE01 displays an aggressive behaviour with an offensive T6SS characterized by continuous and intense T6SS firing activity. Interestingly, we observed that T6SS firing is correlated with rounding of Pectobacterium cells, suggesting delivery of a potent cell wall targeting effector. Mutagenesis coupled with functional assays then revealed that a putative T6SS secreted amidase, Tae3Pf, is mainly responsible for MFE01 toxicity towards Pca. Further studies finally demonstrated that Tae3Pf is toxic when produced in the periplasm, and that its toxicity is counteracted by the Tai3Pf inner membrane immunity protein.
A systematic review of interventions to increase the use of smoking cessation services for women who smoke during pregnancy
Authors: Bailey Cheryl , Medeiros Poliana de Barros , Ellwood David A. , Middleton Philippa , Andrews Christine J. , Flenady Vicki J. 
Published: August 2023

BackgroundAlthough many pregnant women accept referrals to stop‐smoking support, the uptake of appointments often remains low.AimThe aim was to review the success of interventions to increase the uptake of external stop‐smoking appointments following health professional referrals in pregnancy.Materials and MethodsEmbase, PubMed, Cochrane Central Register of Controlled Trials, Scopus and CINAHL were searched in February 2023 for studies with interventions to increase the uptake rates of external stop‐smoking appointments among pregnant women who smoke. Eligible studies included randomised, controlled, cluster‐randomised, quasi‐randomised, before‐and‐after, interrupted time series, case–control and cohort studies. Cochrane tools assessing for bias and Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines were followed.ResultsTwo before‐and‐after studies were included, including a combined total of 1996 women who smoked during pregnancy. Both studies had a serious risk of bias, and meta‐analysis was not possible due to heterogeneity. One study testing carbon monoxide monitors and opt‐out referrals showed increased uptake of external stop‐smoking appointments, health professional referrals and smoking cessation rates compared to self‐identified smoking status and opt‐in referrals. Results were limited in the second study, which used carbon monoxide monitors, urinary cotinine levels and self‐disclosed methods to identify the smoking status with opt‐out referrals. Only post‐intervention data were available on the uptake of appointments to external stop‐smoking services. The number of health professional referrals increased, but change in smoking cessation rates was less clear.ConclusionsThere is insufficient evidence to inform practice regarding strategies to increase the uptake of external stop‐smoking appointments by women during pregnancy.
Global proteomics reveals insulin abundance as a marker of human islet homeostasis alterations
Authors: Mathisen Andreas F. , Abadpour Shadab , Legøy Thomas Aga , Paulo Joao A. , Ghila Luiza , Scholz Hanne , Chera Simona 
Published: August 2023

AbstractAimThe variation in quality between the human islet samples represents a major problem for research, especially when used as control material. The assays assessing the quality of human islets used in research are non‐standardized and limited, with many important parameters not being consistently assessed. High‐throughput studies aimed at characterizing the diversity and segregation markers among apparently functionally healthy islet preps are thus a requirement. Here, we designed a pilot study to comprehensively identify the diversity of global proteome signatures and the deviation from normal homeostasis in randomly selected human‐isolated islet samples.MethodsBy using Tandem Mass Tag 16‐plex proteomics, we focused on the recurrently observed disparity in the detected insulin abundance between the samples, used it as a segregating parameter, and analyzed the correlated changes in the proteome signature and homeostasis by pathway analysis.ResultsIn this pilot study, we showed that insulin protein abundance is a predictor of human islet homeostasis and quality. This parameter is independent of other quality predictors within their acceptable range, thus being able to further stratify islets samples of apparent good quality. Human islets with low amounts of insulin displayed changes in their metabolic and signaling profile, especially in regard to energy homeostasis and cell identity maintenance. We further showed that xenotransplantation into diabetic hosts is not expected to improve the pre‐transplantation signature, as it has a negative effect on energy balance, antioxidant activity, and islet cell identity.ConclusionsInsulin protein abundance predicts significant changes in human islet homeostasis among random samples of apparently good quality.
Prognostic impact of FLT3‐ITD mutation on NPM1+ acute myeloid leukaemia patients and related molecular mechanisms
Authors: Pan Xin’an , Chang Yingjun , Ruan Guorui , Wei Fangfang , Jiang Hao , Jiang Qian , Huang Xiaojun , Zhao Xiaosu 
Published: August 2023

SummaryThe prognosis of acute myeloid leukaemia (AML) patients carrying NPM1 mutations is significantly worse when accompanied by FLT3‐ITD mutations. However, accurate quantitative detection of FLT3‐ITD mutations remains challenging. To identify a novel biomarker in NPM1+FLT3‐ITD+ AML patients for more accurate stratification, we analysed the differential gene expression between the NPM1+FLT3‐ITD+ and NPM1+FLT3‐ITD− groups in five public AML datasets and identified a biomarker by taking the intersection of differentially expressed genes. We validated this biomarker in bone marrow samples from NPM1+ AML patients at the Peking University Institute of Haematology and analysed its prognostic significance. BCAT1 expression was higher in the NPM1+FLT3‐ITD+ group than in the NPM1+FLT3‐ITD− group in all seven cohorts. BCAT1 was able to predict the prognosis of NPM1+FLT3‐ITD+ AML patients, and its predictive ability was superior to that of the FLT3‐ITD allelic ratio (AR). FLT3‐targeted inhibitor quizartinib reduced BCAT1 expression. BCAT1 knockdown using lentiviral vectors led to the downregulation of MYC expression. Thus, we identified BCAT1 as a novel biomarker for NPM1+FLT3‐ITD+ AML patients. The FLT3‐ITD/BCAT1/MYC signalling pathway may play a biological role in promoting the occurrence and development of AML in FLT3‐ITD+ cell lines.
Using prostate‐specific membrane antigen positron‐emission tomography to guide prostate biopsies and stage men at high‐risk of prostate cancer
Authors: Bodar Yves J.L. , Boevé Liselotte M.S. , van Leeuwen Pim J. , Baars Phillippe C. , Nieuwenhuijzen Jakko A. , van Haarst Ernst P. , Oddens Jorg R. , Donswijk Maarten L. , van Riel Luigi A. M. J. G. , Scheltema Matthijs J. , Meijer Dennie , Hendrikse N. Harry , Oprea‐Lager Daniela E. , Vis André N. 
Published: August 2023

ObjectiveTo assess whether a diagnostic pathway in which prostate‐specific membrane antigen (PSMA) positron‐emission tomography (PET)/computed tomography (CT) is used as a single imaging modality is feasible to guide targeted biopsy and to detect clinically significant prostate cancer (csPCa) in biopsy‐naïve men at high‐risk of disease.Patients and MethodsA total of 60 men with a prostate‐specific antigen (PSA) level of 20–50 ng/mL underwent 18F‐PSMA(DCFPyL)‐PET/CT prior to prostate biopsies in this prospective, non‐randomised cohort study. Magnetic resonance imaging (MRI) was not performed. Using a 12‐segment mapping model of the prostate, PSMA‐guided targeted biopsy was performed along with systematic biopsies. The detection rate of PCa and csPCa was assessed for combined systematic and targeted biopsy, and for targeted biopsy only. csPCa was defined as a prostate biopsy with an International Society of Uropathology (ISUP) Grade Group ≥2.ResultsLesions suspicious for PCa in the prostate gland were observed on all PSMA‐PET/CTs. A total of 27/60 men (45%) already had metastatic disease on staging 18F‐PSMA(DCFPyL)‐PET/CT. Combined PSMA‐guided targeted and systematic biopsies detected PCa in 56/60 (93.3%) patients, with 52 of them (92.9%) having csPCa. PSMA‐guided targeted biopsy, if performed as a single biopsy modality, identified PCa in 52/60 men (86.7%) and in 27/27 men (100%) men with metastases.ConclusionsUsing the PSMA‐driven single imaging modality pathway in biopsy‐naïve men at high‐risk of PCa, a substantial number of diagnostic MRI scans could be avoided while at the same time obtaining adequate targeting, staging, and detection of csPCa.
Gut feelings in the islets: The role of the gut microbiome and the FFA2 and FFA3 receptors for short chain fatty acids on β‐cell function and metabolic regulation
Authors: Teyani Razan , Moniri Nader H. 
Published: August 2023

AbstractShort‐chain fatty acids (SCFAs) are biosynthesized via fermentation of polysaccharides by gastrointestinal microbiota and have been shown to have wide‐reaching effects on almost all tissues, including the pancreatic islets. Historically, the effects of SCFAs have been attributed to their intracellular metabolism and function as energy sources, but the discovery of free fatty acid G protein‐coupled receptors (GPCRs) in the 2000s suggested that many functional outcomes of SCFAs are receptor‐mediated. The SCFA receptors FFA2/GPR43 and FFA3/GPR41 are expressed on β‐cells, where they regulate glucose‐dependent insulin secretion, making them attractive targets for treatment of diabetes and other metabolic disorders. Here, we provide an update on the current evidence regarding regulation of FFA2/FFA3 receptors by specific probiotic bacterial species within the gut microbiome that synthesize SCFAs. We also review the body of research regarding the FFA2‐ and FFA3 receptor‐specific function of SCFAs on β‐cells and discuss the somewhat controversial and opposing findings within these studies.
Joint application of fluid inclusion and clumped isotope (∆47) thermometry unravels the complexity of thermal and fluid‐flow history reconstruction in sedimentary basins—Upper Triassic Chaunoy Formation reservoirs (Paris Basin)
Authors: Vergara Sassarini Natalia A. , Gasparrini Marta , Bernasconi Stefano M. , Corrado Sveva 
Published: August 2023

AbstractWe investigated calcites and dolomites precipitated during burial diagenesis of the Upper Triassic (Norian) continental siliciclastics from sub‐surface reservoirs of the northern Paris Basin (Chaunoy Formation) that experienced a thermal maximum >100°C during Late Cretaceous times. Relative carbonate precipitation timing was established via petrographic analyses. The diagenetic carbonates were further investigated by fluid inclusion and clumped isotope (Δ47) thermometry. The two thermometric datasets were interpreted by evaluating the possible occurrence of inclusion thermal reequilibration and Δ47 solid‐state reordering, based on the known basin thermal history and the three existing Δ47 reordering models. By considering the fluid inclusion and Δ47 datasets obtained and the various Δ47 reordering models, different carbonate precipitation scenarios, in terms of timing and parent fluid composition (δ18Ofluid), were inferred. These results underline that in samples having experienced thermal maximum >100°C, accuracy and interpretation of fluid inclusion and Δ47 thermometry data (especially on calcite) may be biased by thermal reequilibration and solid‐state reordering. The results converge towards the need of jointly applying fluid inclusion and Δ47 thermometry on the same carbonate phases to evaluate all the possible precipitation scenarios. The most likely carbonate precipitation scenarios, based on Δ47 thermometry data, point at the precipitation of two calcite phases during Early to Late Jurassic times and of one dolomite phase during the Late Cretaceous. The parent fluids possibly were original formation waters of the Chaunoy Fm. that mixed with brines migrating from the East, where time equivalent evaporitic deposits occur. The proposed precipitation model for calcites and dolomites, involving different pulses of brine migration, and the dominance of calcite phases were not recorded by previous studies on the Upper Triassic units. These latter results may be of interest to evaluate the reservoir potential of the Chaunoy Fm. in this underexplored portion of the Paris Basin.
An integrative approach to assess the impact of disturbance on native fish in lakes
Authors: Thomson‐Laing Georgia , Schallenberg Lena , Kelly David , Howarth Jamie D. , Wood Susanna A. 
Published: August 2023

ABSTRACTFreshwater fish are in a perilous state with more than 30% of species considered critically endangered. Yet significant ecological and methodological complexities constrain our ability to determine how disturbances are impacting native fish communities. We review current methods used to assess the responses of fish communities, especially native fish, to disturbances, with a focus on lakes. These methods include contemporary population surveys, manipulative experimental approaches, paleolimnological approaches and Indigenous Knowledge and social histories. We identify knowledge gaps, such as a lack of baseline data for native fish, an inability to assess the impact of historical disturbances, stressor response dynamics in contemporary multi‐stressor environments, and natural disturbance regimes. Our assessment of the current methods highlights challenges to filling these knowledge gaps using the reviewed methods. We advocate strongly for the implementation of an integrative approach that combines emerging technologies (i.e. molecular‐based techniques in contemporary surveys and paleolimnology) and underutilised knowledge streams (i.e. Indigenous Knowledge and social histories) which should be used in concert with conventional methods. This integrative approach will allow researchers to determine the key drivers of decline and the degree of change, which will enable more informed and successful management actions.
The value of communal and intergenerational settings for studying social and emotional learning
Authors: Mesinas Melissa , Casanova Saskias 
Published: August 2023

AbstractIndigenous Latinx youth living in the United States are culturally diverse individuals whose experiences are often unrecognized. These diasporic youth identify, learn, and develop cultural strengths amid their upbringing outside their Native communities by engaging in integrated communal endeavors informed by Indigenous values. In this article, we present research on how interconnected aspects of diasporic Indigenous practices contribute to the social and emotional development of youth in middle childhood and adolescence. We focus on Indigenous familial and communal experiences that contribute to developmental processes. We provide nuanced perspectives to studying social and emotional learning (SEL) outside the traditional school setting by expanding to communal contexts. We examinecomunalidad(communality) and intergenerational practices to consider how transformative SEL can be more inclusive of Indigenous ways of knowing and living. Our focus is the cultural strengths these communities bring to the SEL competencies of relationships and social awareness, which are applicable to the development of all children.
PGAM5 knockout causes depressive‐like behaviors in mice via ATP deficiency in the prefrontal cortex
Authors: Cui Weiwei , Chen Chunhui , Gong Liya , Wen Junyan , Yang Shanshan , Zheng Min , Gao Baogui , You Junxiong , Lin Xuecong , Hao Yanyu , Chen Zhimin , Wu Ziqi , Gao Liaoming , Tang Jiayu , Yuan Zhen , Sun Xuegang , Jing Linlin , Wen Ge 
Published: August 2023

AbstractIntroductionMajor depressive disorder (MDD) affects about 17% population in the world. Although abnormal energy metabolism plays an important role in the pathophysiology of MDD, however, how deficiency of adenosine triphosphate (ATP) products affects emotional circuit and what regulates ATP synthesis are still need to be elaborated.AimsOur study aimed to investigate how deficiency of PGAM5‐mediated depressive behavior.ResultsWe firstly discovered that PGAM5 knockout (PGAM5−/−) mice generated depressive‐like behaviors. The phenotype was reinforced by the observation that chronic unexpected mild stress (CUMS)‐induced depressive mice exhibited lowered expression of PGAM5 in prefrontal cortex (PFC), hippocampus (HIP), and striatum. Next, we found, with the using of functional magnetic resonance imaging (fMRI), that the functional connectivity between PFC reward system and the PFC volume were reduced in PGAM5−/− mice. PGAM5 ablation resulted in the loss of dendritic spines and lowered density of PSD95 in PFC, but not in HIP. Finally, we found that PGAM5 ablation led to lowered ATP concentration in PFC, but not in HIP. Coimmunoprecipitation study showed that PGAM5 directly interacted with the ATP F1F0 synthase without influencing the interaction between ATP F1F0 synthase and Bcl‐xl. We then conducted ATP administration to PGAM5−/− mice and found that ATP could rescue the behavioral and neuronal phenotypes of PGAM5−/− mice.ConclusionsOur findings provide convincing evidence that PGAM5 ablation generates depressive‐like behaviors via restricting neuronal ATP production so as to impair the number of neuronal spines in PFC.
Efficacy of treatment with N‐acetylcysteine inhalation for AECOPD: A propensity‐score‐matched cohort study
Authors: Chen Hengyi , Zhou Hui , Luo Chen , Zong Kaican , Fu Yingya , Li Wen , Luo Chunyan , Xue Guojuan , Jiang E. , Duan Yang , Luo Tinglan , Jiang Yangzhi 
Published: August 2023

AbstractIntroductionN‐acetylcysteine (NAC) prevents acute exacerbations of chronic obstructive pulmonary disease (AECOPD). However, the value of NAC inhalation in the treatment of patients with AECOPD is still poorly understood. The study was conducted to evaluate the efficacy of NAC inhalation in AECOPD patients requiring hospitalization.MethodsIn this single institutional, retrospective cohort study, all patients with AECOPD requiring hospitalization between January 2021 and January 2022 were included. Patients were divided into NAC group and Non‐NAC group according to whether being treated with NAC inhalation and were matched using the propensity score. The primary outcome was a composite of progression to ventilation requirement, in‐hospital mortality and readmission for AECOPD within 30 days. The effect on the mean hospitalized days, blood gas indexes and the incidence rate of adverse drug events were compared between the two groups.ResultsNinety‐six patients in the NAC group were matched with 96 patients in the Non‐NAC group. The differences in the primary composite end point (NAC group vs Non‐NAC group, 5.2% vs 16.7%; P = 0.011) were significant. The median time to discharge was shorter in the NAC group (8.3 vs. 9.1 days, P = 0.030). The NAC group presented a larger increase in partial pressure of arterial oxygen (PaO2) and a higher ratio of self‐reported symptomatic improvement from admission to day 5. There was no definite difference between the two groups in the frequency of adverse event.ConclusionNAC inhalation is associated with an improved clinical outcome. A further study should be conducted to confirm the clinical usefulness of NAC inhalation in AECOPD patients.