bluuebunny/crossref_metadata_2025_split huggingface dataset:
The development of folate-functionalised palladium nanoparticles for folate receptor targeting in breast cancer cells
Authors: Gandidzanwa Sendibitiyosi , Beukes Natasha , Joseph Sinelizwi V , Janse Van Vuuren Arno , Mashazi Philani , Britton Jonathan , Kilian Gareth , Roux Saartjie , Nyokong Tebello , Lee Michael E , Frost Carminita L , Tshentu Zenixole R 
Published: August 2023

Abstract
Folate receptor-targeted therapy has excellent prospects for the treatment of breast cancer. A non-toxic concentration of folate-conjugated palladium-based nanoparticles was used to target the overexpressed folate receptor on breast cancer cells. The folate-conjugated nanoparticles were tailored to accumulate selectively in cancer cells relative to normal cells via the folate receptor. The MDA-MB-231, MDA-MB-468, MCF-7 breast cancer cell lines, and MCF-10A normal cell lines were used in the study. Qualitative and quantitative analysis of nanoparticle cellular uptake and accumulation was conducted using transmission electron microscopy and inductively coupled plasma-optical emission spectroscopy. The findings proved that folate-conjugated palladium nanoparticles successfully and preferentially accumulated in breast cancer cells. We conclude that folate-conjugated palladium nanoparticles can be potentially used to target breast cancer cells for radiopharmaceutical applications.
Data-driven reconstruction of stochastic dynamical equations based on statistical moments
Authors: Nikakhtar Farnik , Parkavousi Laya , Sahimi Muhammad , Reza Rahimi Tabar M , Feudel Ulrike , Lehnertz Klaus 
Published: August 2023

Abstract
Stochastic processes are encountered in many contexts, ranging from generation sizes of bacterial colonies and service times in a queueing system to displacements of Brownian particles and frequency fluctuations in an electrical power grid. If such processes are Markov, then their probability distribution is governed by the Kramers–Moyal (KM) equation, a partial differential equation that involves an infinite number of coefficients, which depend on the state variable. The KM coefficients must be evaluated based on measured time series for a data-driven reconstruction of the governing equations for the stochastic dynamics. We present an accurate method of computing the KM coefficients, which relies on computing the coefficients’ conditional moments based on the statistical moments of the time series. The method’s advantages over state-of-the-art approaches are demonstrated by investigating prototypical one-dimensional stochastic processes with well-known properties.
Parity violating gravitational waves at the end of inflation
Authors: Bastero-Gil Mar , Torres Manso António 
Published: August 2023

AbstractInflaton-vector interactions of the typeϕFF̃have provided interesting phenomenology to tackle some of current problems in cosmology, namely the vectors could constitute the dark matter component. It could also lead to possible signatures imprinted in a gravitational wave spectrum. Through this coupling, a rolling inflaton induces an exponential production of the transverse polarizations of the vector field, having a maximum at the end of inflation when the inflaton field velocity is at its maximum. These gauge particles, already parity asymmetric, will source the tensor components of the metric perturbations, leading to the production of parity violating gravitational waves. In this work we examine the vector particle production in the weak coupling regime, integrating the gauge mode amplitudes spectrum during the entirety of its production and amplification epochs, until the onset of radiation domination. Finally, we calculate the gravitational wave spectrum combining the vector mode analytical solution, the WKB expansion, valid only during the amplification until horizon crossing, and the numerical solution obtained at the beginning of radiation domination when the modes cease to grow.
120 mA operation of J-PARC cesiated RF-driven H- ion source
Authors: Ueno A. , Ohkoshi K. , Ikegami K. , Oguri H. 
Published: August 2023

Abstract
The Japan Proton Accelerator Research Complex (J-PARC) cesiated RF-driven H- ion source (IS) has been stably operated for about eight years by using an internal-RF-antenna developed at the Spallation Neutron Source (SNS). From Sep. 2018, the J-PARC 400 MeV LINAC has been steadily operated with the design H- ion beam intensity (IH-
) of 50 mA, when an about 60 mA beam is injected from the IS. At each end of about 3 months J-PARC operation, the IS supplies a 52.5 keV 72 mA beam for the LINAC 60 mA acceleration. The IS superior emittances predicted in a test-stand were proven by the high RFQ acceleration efficiency of 94.3% (IH-RFQ = 67.9 mA). The high intensity beam with transverse emittances suitable for the RFQ is produced with several unique measures, such as, slight water molecules addition into hydrogen plasma, the low temperature (about 70°C) operation of 45°-tapered 16-mm thick plasma electrode with the precise cesium (Cs) density control, the impurity elimination in the hydrogen plasma along with the filter-field optimization, the continuous-wave igniter plasma driven with a 42-W 30-MHz RF, and so on. The 120 mA operation results of the IS in the test-stand are presented in this paper. A 69.9 keV 120 mA H- ion beam, about 107.8 mA of which was inside the transverse emittances used for a common RFQ design, was stably operated with a duty factor of 4% (1 ms × 40 Hz). The IS is the benchmark one for the next generation high energy H- LINACs aiming for 100 mA class intensity.
Camelid-derived CD38 antibody successfully circumvents epitope blockade by the therapeutic anti-CD38 antibody daratumumab
Authors: ElMaraashly Aya Hassan , Tario Joseph , Hillengass Jens , Qian You-Wen 
Published: August 2023

Abstract

Objectives
To evaluate the efficacy of an anti-CD38 nanobody to detect plasma cells in a flow cytometry myeloma minimal residual disease (MRD) panel in patients treated with daratumumab and other immunotherapies.

Methods
Twenty-three bone marrow samples from as many patients were collected during or at the end of daratumumab treatment cycles. A 5-tube, 8-color flow cytometry MRD panel was performed. Dotplots were reviewed, and the median fluorescence intensity (MFI) was calculated.

Results
Patients’ ages ranged from 45 to 77 years, and the cohort was made up of 13 men and 10 women who had undergone 2 to 24 cycles of daratumumab therapy at the time of myeloma MRD testing. In all 23 cases, therapeutic use of daratumumab impaired pathologists’ ability to measure CD38 on plasma cells when using a conventional murine monoclonal antibody (anti-CD38 fluorescein isothiocyanate [FITC], clone T16; Beckman Coulter). In 21 of the 23 cases, the measurement of CD38 was restored when the anti-CD38 nanobody was employed. Compared with anti-CD38 FITC, the anti-CD38 Alexa Fluor 488 nanobody (Beckman Coulter) produced higher MFI and allowed measurement of a higher frequency of discernable plasma cells.

Conclusions
The camelid-derived CD38 antibody successfully circumvents the steric inhibition of CD38 that the therapeutic use of daratumumab imparts and facilitates myeloma MRD plasma cell detection.
Anthropometric Measurements and Analysis for Objective Assessment of Gynecomastia Surgery Results
Authors: Ramasamy Karthik , Shet Srivatsa M , Patil Pankaj , Tripathee Sanjib , Dhevi Nanthini , Alagarasan Abisshek Raj 
Published: August 2023

Abstract

Background
Gynecomastia surgery is one of the most common aesthetic procedures in males. There is a lack of objective analytical parameters to judge outcomes. In this study, the authors aim to introduce novel anthropometric measurements and analysis techniques for the objective assessment of surgical outcomes based on specific aesthetic targets.

Objectives
To introduce quantification of gynecomastia surgery outcomes and compare the results among the different grades of gynecomastia.

Methods
A total of 192 patients with gynecomastia were included. The patient cases were grouped according to grades and a set of anthropometric measurements were taken both before the operation and 6 months postoperatively. Liposuction and glandular excision were done through minimal incisions in all grades of gynecomastia, with the addition of ultrasound and nipple areola complex (NAC) lifting plaster in selected Grade 3 and all Grade 4 cases.

Results
A statistically significant improvement in the perimeter of the triangular relationship of sternal notch and nipples, the elevation of the NAC, the reduction of the area of the NAC, and the correction of asymmetry of the chest were seen in all grades of gynecomastia, with increased differences in higher grades.

Conclusions
A systematic objective analysis of the specific aesthetic targets helps to reliably compare results in a standard way and for carrying out improvisation of surgeons’ techniques. Meanwhile, this approach helps identifying the need for customization, eventually providing symmetric and aesthetically pleasing surgical results.

Level of Evidence: 3
Repeated mild traumatic brain injury triggers pathology in asymptomatic C9ORF72 transgenic mice
Authors: Kahriman Aydan , Bouley James , Tuncali Idil , Dogan Elif O , Pereira Mariana , Luu Thuyvan , Bosco Daryl A , Jaber Samer , Peters Owen M , Brown Robert H , Henninger Nils 
Published: August 2023

Abstract
Frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) are fatal neurodegenerative diseases that represent ends of the spectrum of a single disease. The most common genetic cause of FTD and ALS is a hexanucleotide repeat expansion in the C9orf72 gene. Although epidemiological data suggest that traumatic brain injury (TBI) represents a risk factor for FTD and ALS, its role in exacerbating disease onset and course remains unclear.
To explore the interplay between traumatic brain injury and genetic risk in the induction of FTD/ALS pathology we combined a mild repetitive traumatic brain injury paradigm with an established bacterial artificial chromosome transgenic C9orf72 (C9BAC) mouse model without an overt motor phenotype or neurodegeneration. We assessed 8–10 week-old littermate C9BACtg/tg (n = 21), C9BACtg/− (n = 20) and non-transgenic (n = 21) mice of both sexes for the presence of behavioural deficits and cerebral histopathology at 12 months after repetitive TBI.
Repetitive TBI did not affect body weight gain, general neurological deficit severity, nor survival over the 12-month observation period and there was no difference in rotarod performance, object recognition, social interaction and acoustic characteristics of ultrasonic vocalizations of C9BAC mice subjected to repetitive TBI versus sham injury. However, we found that repetitive TBI increased the time to the return of the righting reflex, reduced grip force, altered sociability behaviours and attenuated ultrasonic call emissions during social interactions in C9BAC mice. Strikingly, we found that repetitive TBI caused widespread microglial activation and reduced neuronal density that was associated with loss of histological markers of axonal and synaptic integrity as well as profound neuronal transactive response DNA binding protein 43 kDa mislocalization in the cerebral cortex of C9BAC mice at 12 months; this was not observed in non-transgenic repetitive TBI and C9BAC sham mice.
Our data indicate that repetitive TBI can be an environmental risk factor that is sufficient to trigger FTD/ALS-associated neuropathology and behavioural deficits, but not paralysis, in mice carrying a C9orf72 hexanucleotide repeat expansion.
Effects of zinc deficiency on the regeneration of olfactory epithelium in mice
Authors: Ikushima Hiroyuki , Suzuki Jun , Hemmi Tomotaka , Ikeda Ryoukichi , Kobayashi Yuta , Ohta Nobuo , Katori Yukio 
Published: August 2023

Abstract
The olfactory epithelium can regenerate after damage; however, the regeneration process is affected by various factors, such as viral infections, head trauma, and medications. Zinc is an essential trace element that has important roles in organ development, growth, and maturation. Zinc also helps regulate neurotransmission in the brain; nevertheless, its relationship with olfactory epithelium regeneration remains unclear. Therefore, we used a severe zinc deficiency mouse model to investigate the effects of zinc deficiency on olfactory epithelium regeneration. Male wild-type C57BL/6 mice were divided into zinc-deficient and control diet groups at the age of 4 weeks, and methimazole was administered at the age of 8 weeks to induce severe olfactory epithelium damage. We evaluated the olfactory epithelium before and 7, 14, and 28 days after methimazole administration by histologically analyzing paraffin sections. RNA sequencing was also performed at the age of 8 weeks before methimazole administration to examine changes in gene expression caused by zinc deficiency. In the zinc-deficient group, the regenerated olfactory epithelium thickness was decreased at all time points, and the numbers of Ki-67-positive, GAP43-positive, and olfactory marker protein-positive cells (i.e. proliferating cells, immature olfactory neurons, and mature olfactory neurons, respectively) failed to increase at some time points. Additionally, RNA sequencing revealed several changes in gene expression, such as a decrease in the expression of extracellular matrix-related genes and an increase in that of inflammatory response-related genes, in the zinc-deficient group. Therefore, zinc deficiency delays olfactory epithelium regeneration after damage in mice.
Impact of SARS-CoV-2 Prevention Measures on Non-SARS-CoV-2 Hospital-Onset Respiratory Viral Infections: An Incidence Trend Analysis From 2015–2023
Authors: Ehrenzeller Selina , Chen Tom , Vaidya Vineeta , Rhee Chanu , Baker Meghan A , Klompas Michael 
Published: August 2023

Abstract
We reviewed hospital-onset respiratory viral infections, 2015–2023, in one hospital to determine whether Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) transmission prevention measures prevented non-SARS-CoV-2 respiratory viral infections. Masking, employee symptom attestations, and screening patients and visitors for symptoms were associated with a 44%–53% reduction in hospital-onset influenza and respiratory syncytial virus (RSV), accounting for changes in community incidence.
Conservation of thermal physiology in tropical intertidal snails following an evolutionary transition to a cooler ecosystem: climate change implications
Authors: Marshall David J , Mustapha Nurshahida , Monaco Cristián J 
Published: August 2023

Abstract
Predictions for animal responses to climate warming usually assume that thermal physiology is adapted to present-day environments, and seldom consider the influence of evolutionary background. Little is known about the conservation of warm-adapted physiology following an evolutionary transition to a cooler environment. We used cardiac thermal performance curves (cTPCs) of six neritid gastropod species to study physiological thermal trait variation associated with a lineage transition from warmer rocky shores to cooler mangroves. We distinguished between functional thermal performance traits, related to energy homeostasis (slope gradient, slope curvature, HRmax, maximum cardiac activity and Topt, the temperature that maximizes cardiac activity) and a trait that limits performance (ULT, the upper lethal temperature). Considering the theory of optimal thermal performance, we predicted that the functional traits should be under greater selective pressure to change directionally and in magnitude than the thermal limit, which is redundant in the cooler environment. We found little variation in all traits across species, habitats and ecosystems, despite a ~20°C reduction in maximum habitat temperature in the mangrove species over 50 million years. While slope gradient was significantly lowered in the mangrove species, the effect difference was negated by greater thermal plasticity in the rocky shore species. ULT showed the least variation and suggested thermal specialization in the warmest habitat studied. The observed muted variation of the functional traits among the species may be explained by their limited role in energy acquisition and rather their association with heat tolerance adaptation, which is redundant in the mangrove species. These findings have implications for the conservation of habitat of intertidal gastropods that transition to cooler environments. Furthermore, they highlight the significance of evolutionary history and physiological conservation when predicting species responses to climate change.
Peripheral Blood DNA Methylation Signatures and Response to Tofacitinib in Moderate-to-severe Ulcerative Colitis
Authors: Joustra Vincent , Li Yim Andrew Y F , van Gennep Sara , Hageman Ishtu , de Waard Tristan , Levin Evgeni , Lauffer Peter , de Jonge Wouter , Henneman Peter , Löwenberg Mark , D’Haens Geert 
Published: August 2023

Abstract

Introduction
Predictive biomarkers for treatment efficacy of ulcerative colitis [UC] treatments are lacking. Here, we performed a longitudinal study investigating the association and potential predictive power of genome-wide peripheral blood [PB] DNA methylation signatures and response to tofacitinib treatment in UC.

Methods
We recruited moderate-to-severe UC patients starting tofacitinib treatment, and measured PB DNA methylation profiles at baseline [T1], after 8 weeks [T2], and in a subset [n = 8] after a median of 20 weeks [T3] using the Illumina Infinium HumanMethylation EPIC BeadChip. After 8 weeks, we distinguished responders [R] from non-responders [NR] based on a centrally read endoscopic response [decrease in endoscopic Mayo score ≥1 or Ulcerative Colitis Endoscopic Index of Severity ≥2] combined with corticosteroid-free clinical and/or biochemical response. T1 PB samples were used for biomarker identification, and T2 and publicly available intraclass correlation [ICC] data were used for stability analyses. RNA-sequencing was performed to understand the downstream effects of the predictor CpG loci.

Results
In total, 16 R and 15 NR patients, with a median disease duration of 7 [4–12] years and overall comparable patient characteristics at baseline, were analysed. We identified a panel of 53 differentially methylated positions [DMPs] associated with response to tofacitinib [AUROC 0.74]. Most DMPs [77%] demonstrated both short- and long-term hyperstability [ICC ≥0.90], irrespective of inflammatory status. Gene expression analysis showed lower FGFR2 [pBH = 0.011] and LRPAP1 [pBH = 0.020], and higher OR2L13 [pBH = 0.016] expression at T1 in R compared with NR.

Conclusion
Our observations demonstrate the utility of genome-wide PB DNA methylation signatures to predict response to tofacitinib.
Artificial Intelligence-assisted Analysis of Pan-enteric Capsule Endoscopy in Patients with Suspected Crohn’s Disease: A Study on Diagnostic Performance
Authors: Brodersen Jacob Broder , Jensen Michael Dam , Leenhardt Romain , Kjeldsen Jens , Histace Aymeric , Knudsen Torben , Dray Xavier 
Published: August 2023

Abstract

Background and Aim
Pan-enteric capsule endoscopy [PCE] is a highly sensitive but time-consuming tool for detecting pathology. Artificial intelligence [AI] algorithms might offer a possibility to assist in the review and reduce the analysis time of PCE. This study examines the agreement between PCE assessments aided by AI technology and standard evaluations, in patients suspected of Crohn’s disease [CD].

Method
PCEs from a prospective, blinded, multicentre study, including patients suspected of CD, were processed by the deep learning solution AXARO® [Augmented Endoscopy, Paris, France]. Based on the image output, two observers classified the patient’s PCE as normal or suggestive of CD, ulcerative colitis, or cancer. The primary outcome was per-patient sensitivities and specificities for detecting CD and inflammatory bowel disease [IBD]. Complete reading of PCE served as the reference standard.

Results
A total of 131 patients’ PCEs were analysed, with a median recording time of 303 min. The AXARO® framework reduced output to a median of 470 images [2.1%] per patient, and the pooled median review time was 3.2 min per patient. For detecting CD, the observers had a sensitivity of 96% and 92% and a specificity of 93% and 90%, respectively. For the detection of IBD, both observers had a sensitivity of 97% and had a specificity of 91% and 90%, respectively. The negative predictive value was 95% for CD and 97% for IBD.

Conclusions
Using the AXARO® framework reduced the initial review time substantially while maintaining high diagnostic accuracy—suggesting its use as a rapid tool to rule out IBD in PCEs of patients suspected of Crohn’s disease.
Role of integrated imaging in the diagnosis of an atypical and unresectable cardiac paraganglioma: a case report
Authors: Cutaia Aldo , Gaetani Clara , Fonio Paolo , Faletti Riccardo 
Published: August 2023

Abstract

Background
Paragangliomas (PGLs) are rare neuroendocrine tumours that originate from extra-adrenal location. Cardiac PGLs can cause severe hypertension, palpitations, and lethal tachyarrhythmias. Diagnosis is based on measurement of plasma or urine metanephrines combined with conventional and nuclear imaging. Effective treatment is represented by surgical resection. We report a case of a 19-year-old patient with recurrent acute pericarditis; integrated imaging detected a large cardiac mass suggestive for PGL.

Case summary
A 19-year-old male suffered pleuritic chest pain and fever for 4 days; electrocardiogram showed inferior ST elevation and transthoracic echocardiography a 2.2 cm pericardial effusion; these findings led to diagnose acute pericarditis. After a relapse of pericarditis, cardiac magnetic resonance and cardiac computed tomography (CCT) were performed, revealing a cardiac mass with radiological features of PGL. Blood and urine tests detected elevated levels of 3-methoxytyramine and chromogranin A. Gallium-68 positron emission tomography confirmed high metabolic activity of the mass. A negative 123-I-MIBG scintigraphy ruled out the possibility of radiometabolic treatment. A second CCT excluded the chance of surgical resection, due to intra-lesional course of the left anterior descending coronary artery. The young patient was referred to a different centre to achieve reduction of the mass, in order to potentially resect it afterwards.

Discussion
Cardiac PGLs are rare tumours with significant morbidity related to norepinephrine secretion. In this case, without typical clinical manifestations and with no chance of surgical resection, integrated imaging played a central role in the differential diagnosis between PGL and other cardiac masses, providing both static and dynamic characterization.
Unexpected high troponin T and I values in a child with hypertrophic cardiomyopathy and acute chest pain: a case report
Authors: van Avezaath Lisanne K , Nijenhuis Hessel P , Muller Kobold Anneke C 
Published: August 2023

AbstractBackgroundElevated troponin T (cTnT) and/or troponin I (cTnI) can be ascribed to multiple causes, mostly resulting from cardiac tissue damage and in lesser numbers resulting from non-cardiac related causes. The presence of macrotroponins is easily overlooked, with potentially negative consequences.Case summaryThis case report presents a case study of a 12-year-old child known to have MYH7 gene–associated hypertrophic cardiomyopathy with acute chest pain combined with an unexpected high cTnT and cTnI. A cardiac cause was deemed unlikely after additional investigation, as these showed no abnormalities. After consulting a laboratory specialist, it could be concluded that the high cTnT and cTnI were a result of macrotroponin complexes, a protein complex consisting of circulating protein and endogenous autoantibodies against that protein, resulting in elevated values with misguiding and uncertain clinical significance.DiscussionAwareness of the existence of macrotroponins could have prevented costly diagnostics and prolonged hospital admission with grave psychological impact, especially in children.