bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep ocean water has significant physiological effects on squid Todarodes pacificus
Authors: Hatano Kaito , Yoshida Masa-Aki , Kitani Yoichiro , Hirayama Jun , Hattori Atsuhiko , Ogiso Shouzo , Watabe Yukina , Sekiguchi Toshio , Tabuchi Yoshiaki , Matsubara Hajime , Urata Makoto , Matsumoto Kyoko , Sakatoku Akihiro , Sriva Ajai K. , Toyota Kenji , Suzuki Nobuo 
Published: January 2023

Abstract
This study is the first to demonstrate that deep ocean water (DOW) has physiological significant effects on squid. After 36 h of rearing squids, those reared with DOW had significantly higher total and free cholesterol levels and lower alanine transaminase activity in hemolymph as compared with those reared with surface water (SSW). SSW rearing also resulted in 6.95% weight loss, while DOW rearing caused only 2.5% weight loss, which might be due to liver metabolism suppression. Furthermore, both monovalent (sodium, chloride, and potassium ions) and divalent (calcium, inorganic phosphorus, and magnesium ions) ions in hemolymph were elevated when reared with DOW compared to those when reared with SSW. A study of genes expressed in the brain revealed that five genes were specifically remarked in DOW rearing. Most altered genes were neuropeptides, including those from vasopressin superfamily. These neuropeptides are involved in cholesterol and/or mineral metabolisms and physiological significant effects on squid. This study is the first report the effects of DOW on cholesterol and mineral metabolism of squid and will contribute to squid aquaculture using DOW.
Primitive Decomposition of Bott-Chern and Dolbeault Harmonic (k,k)-Forms on Compact almost Kähler Manifolds
Authors: Holt Tom , Piovani Riccardo 
Published: January 2023

Abstract
Note: Please see pdf for full abstract with equations.
We consider the primitive decomposition of ∂,∂, Bott-Chern and Aeppli-harmonic (k, k)-forms on compact almost Kähler manifolds (M, J,ω). For any D ∈ {∂, ∂,BC,A}, it is known that the LkP0,0 component of ψ ∈ HDk,k is a constant multiple of ωk up to real dimension 6. In this paper we generalise this result to every dimension. We also deduce information on the components Lk−1P1,1 and Lk−2P2,2 of the primitive decomposion. Focusing on dimension 8, we give a full description of the spaces HBC2,2 and HA2,2, from which follows HBC2,2 ⊆ H∂2,2 and HA2,2 ⊆ H∂2,2. We also provide an almost Kähler 8-dimensional example where the previous inclusions are strict and the primitive components of an harmonic form ψ ∈ HDk,k are not D-harmonic, showing that the primitive decomposition of (k, k)-forms in general does not descend to harmonic forms.
Climatology of near-surface wind speed from observations and high-resolution climate modelsover the Tibetan Plateau
Authors: Minola Lorenzo , Zhang Gangfeng , Ou Tinghai , Kukulies Julia , Curio Julia , Guijarro Jose A. , Deng Kaiqiang , Azorin-Molina Cesar , Shen Cheng , Pezzoli Alessandro , Chen Deliang 
Published: January 2023

Abstract
As near-surface wind speed plays a key role in regulating surface evaporation and thus the hydrological cycle, it is crucial to explore its spatio-temporal characteristics. However, in-situ measurements are scarce over the Tibetan Plateau, limiting the understanding of wind speed climate across this high-elevation region. This study explores the climatology of near-surface wind speed over the Tibetan Plateau by using for the first time homogenized observations together with reanalysis products and regional climate model simulations. Measuring stations across the center and the West of the plateau are at higher elevations and display higher mean and standard deviation, confirming that wind speed increases with increasing altitude. By exploring wind characteristics (e.g. seasonal cycle) through cluster analysis, three regions of different wind regimes can be identified: (1) the center of the Tibetan Plateau, characterized by high elevation; (2) the East and the edges of the plateau; and (3) the topographic depression of the Qaidam basin, strongly influenced by the blocking effect of the surrounding mountainous terrain. Owing to the improvements in model physics and data assimilation, the ERA5 reanalysis shows closer agreement to the measured wind conditions than its predecessor ERA-Interim, by being able to reproduce the same three wind regimes. However, the newest ERA5-Land product does not show improvements compared to ERA5 as they share most of the parametrizations. In addition, the two dynamical downscalings of ERA5 analyzed here are not able to capture the observed wind statistics, showing evident biases and discrepancies also when investigating the diurnal variations. Over a region of such complex terrain, the ability of dynamical downscaling in simulating near-surface wind speed may be enhanced by improving the planetary boundary layer parameterization scheme and by better taking into account unresolved topographic features.
Reversible lewisite adsorption/desorption on the transition metal doped graphene: First-principle calculations
Authors: Liu Zhenhua , Liu Huiling , Wang Haitao , Sun Hao 
Published: January 2023

AbstractArsenical compound lewisite was developed to be a potent chemical warfare agent in the blister agent class and was abandoned in the war areas. Exposure to lewisite can cause serious damage to human’s skin, eyes and respiratory tract. Therefore, it is essential for scientific researchers to design materials that can detect and remove the abandoned lewisite efficiently. In the present work, the potential of transition metals doped (TM = Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu and Zn) graphene (TM/G) to eliminate lewisite is investigated by the first-principles calculations based on density functional theory. The adsorption geometry, adsorption energy, charge transfer, density of states and UV spectra of adsorption system of lewisite on TM/G (L@TM/G)are calculated and analyzed. Computational results demonstrate that there is a strong chemical interaction between TM/G substrate and lewisite molecule. More importantly, the adsorption of lewisite on TM/G can be regulated by introducing an electric field with proper direction and intensity, and therefore the reversible adsorption/desorption can be achieved. In addition, the electronic and optical properties of TM/G change significantly after lewisite adsorption, making TM/G promising to detect lewisite agent. The work predicts that TM/G is a potential sensor and renewable adsorbent for lewisite.
Multiple ParA/MinD ATPases coordinate the positioning of disparate cargos in a bacterial cell
Authors: Pulianmackal Lisa , Limcaoco Jose Miguel , Ravi Keerthikka , Yang Sinyu , Zhang Jeffrey , Tran Mimi , O'Meara Matthew , Vecchiarelli Anthony 
Published: January 2023

Abstract
In eukaryotes, linear motor proteins govern intracellular transport and organization. In bacteria, where linear motors are absent, the ParA/MinD (A/D) family of ATPases spatially organize an array of genetic- and protein-based cellular cargos. ParA is well known to segregate plasmids and chromosomes, as is MinD for its role in divisome positioning. Less studied is the growing list of ParA/MinD-like ATPases found across prokaryotes and involved in the spatial organization of diverse protein-based organelles, such as Bacterial Microcompartments (BMCs), flagella, chemotaxis clusters, and conjugation machinery. Given the fundamental nature of these processes in both cell survival and pathogenesis, the positioning of these cargos has been independently investigated to varying degrees in several organisms. However, it remains unknown whether multiple A/D ATPases can coexist and coordinate the positioning of such a diverse set of fundamental cargos in the same cell. If so, what are the mechanistic commonalities, variation, and specificity determinants that govern the positioning reaction for each cargo? Here, we find that over a third of sequenced bacteria encode multiple A/D ATPases. Among these bacteria, we identified several human pathogens as well as the experimentally tractable organism, Halothiobacillus neapolitanus, which encodes seven A/D ATPases. We directly demonstrate that five of these A/D ATPases are each dedicated to the spatial regulation of a single cellular cargo: the chromosome, the divisome, the carboxysome BMC, the flagellum, and the chemotaxis cluster. We identify putative specificity determinants that allow each A/D ATPase to position its respective cargo. Finally, we show how the deletion of one A/D ATPase can have indirect effects on the inheritance of a cargo actively positioned by another A/D ATPase, stressing the importance of understanding how organelle trafficking, chromosome segregation, and cell division are coordinated in bacterial cells. Together, our data show how multiple A/D ATPases coexist and function to position a diverse set of fundamental cargos in the same bacterial cell. With this knowledge, we anticipate the design of minimal autonomous positioning systems for natural- and synthetic-cargos in bacteria for synthetic biology and biomedical applications.
Information theory methods for quantifying diagnostic heterogeneity in psychopathology
Authors: Buss John F. , Watts Ashley L. , Lorenzo-Luaces Lorenzo 
Published: January 2023

Abstract
Objectives: Although specifiers for a major depressive disorder (MDE) are supposed to reduce diagnostic heterogeneity, recent literature challenges the idea that the atypical and melancholic features identify more homogenous or coherent subgroups. We attempt to replicate these findings and explore whether symptom heterogeneity is reduced in depression subgroups using novel data-analytic techniques.
Methods: Using data derived from the National Epidemiological Survey on Alcohol and Related Conditions (NESARC Wave I; N = 5,749) and Sequenced Treatment Alternatives to Relieve Depression (STAR*D; N = 2,498) we computed the Hamming and Manhattan distance ratios comparing within and between individuals for the melancholic and atypical specifier subgroups.
Results: In neither of the datasets was the heterogeneity between subgroups higher than the heterogeneity within subgroups, suggesting that the melancholic and atypical specifiers do not create more coherent (i.e., more homogeneous) subgroups.
Conclusion: Replicating prior work, melancholic and atypical depression subtypes appear to have limited utility in reducing heterogeneity. The current study does not support the claim that symptom and course specifiers create more coherent subgroups as operationalized by similarity in symptoms and their severity.
A Photochemical Study of Photo-induced Electron Transfer from DNAs to a Cationic Phthalocyanine Derivative
Authors: Homma Shiori , Momotake Atsuya , Ikeue Takahisa , Yamamoto Yasuhiko 
Published: January 2023

Abstract
Water-soluble phthalocyanines (Pcs) have emerged as promising photosensitizers for photodynamic therapy (PDT). Elucidation of their photochemical and photophysical properties, such as the photogeneration of reactive oxygen species (ROSs) and photocytotoxicity, is essential for the molecular design of PDT photosensitizing agents. Water-soluble cationic gallium(III)-Pc complex (GaPc) is capable of photogenerating ROSs in vitro and is well taken up by cells but does not exhibit photocytotoxicity in vivo. GaPc binds selectively, through a π-π stacking interaction, to the 5’-terminal G-quartet of a G-quadruplex DNA. The photo-excited state of GaPc of the complex is effectively quenched through electron transfer (ET) from the ground state of DNA guanine (G) bases to the photo-excited state of GaPc (ET(G−GaPc)). Hence the loss of the photocytotoxicity of GaPc in vivo is most likely to be due to the effective quenching of its photo-excited state through ET(G−GaPc). In this study, we investigated the photochemical properties of GaPc in the presence of duplex DNAs formed from a series of sequences to elucidate the nature of ET(G−GaPc). We found that ET(G−GaPc) is allowed in electrostatic complexes between GaPc and G-containing duplex DNAs and that the rate of ET(G−GaPc) (kET(G−GaPc)) can be reasonably interpreted in terms of the distance between Pc moiety of GaPc and DNA G base in the complex. These results indicated that photocytotoxicity of GaPc is crucially affected by ET(G−GaPc). Thus elucidation of interaction of a photosensitizer with biomolecules, i.e., an initial process in PDT, would be helpful to understand its subsequent photochemical processes.
Epiphytic and Endophytic Bacteria on Camellia oleifera Phyllosphere: Exploring Region and Cultivar Effect.
Authors: Chen Xiaolin , Li Lili , He Yuanhao 
Published: January 2023

Abstract
The epiphytic and endophytic bacteria play an important role in the healthy growth of plants. Both plant species and environmental characteristics influence the bacterial population diversity, yet it is inconclusive whether it is the former or the latter that has a greater impact. To explore the communities of the epiphytic and endophytic microbes in Camellia oleifera, this study assessed three representative C. oleifera cultivars from three areas in Hunan, China by Illumina high-throughput sequencing. The results showed that the diversity and species richness of endophytic microbial community in leaves were significantly higher than those of microbial community in the epiphytic. The diversity and species richness of epiphytic and endophytic microbes are complex when the same cultivar was grown in different areas. The C. oleifera cultivars grown in Youxian had the highest diversity of epiphytic microbial community, but the lowest abundance, while the cultivars grown in Changsha had the highest diversity and species richness of endophytic microbes in leaves. It was concluded that the dominant flora mainly included Proteobacteria, Actinobacteriota and Firmicutes through the analysis of the epiphytic and endophytic microbial communities of C. oleifera. The species and relative abundances of epiphytic and endophytic microbial community were extremely different at the genus level. The analysis of NMDS map and PERMANOVA shows that the species richness and diversity of microbial communities in epiphytes are greatly influenced by environmental characteristics. However, the community structure of endophytic microorganisms in leaves is influenced by region and cultivated varieties, but the influence of region is more significant. Molecular ecological network analysis showed that the symbiotic interaction of epiphytic microbial community was more complex.
Time-dependent effect of GLP-1 receptor agonists on cardiovascular benefits: a real-world study
Authors: Piccini Sara , Favacchio Giuseppe , Panico Cristina , Morenghi Emanuela , Folli Franco , Mazziotti Gherardo , Lania Andrea Gerardo , Mirani Marco 
Published: January 2023

Abstract
Background: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) have shown cardiovascular benefits in cardiovascular outcome trials in type 2 diabetes mellitus. However, the most convincing evidence was obtained in subjects with established cardiovascular (CV) disease. We analyzed the determinants of GLP-1 RA-mediated CV protection in a real-world population of persons with type 2 diabetes with and without a history of CV events with long-term follow-up.
Methods: Retrospective cohort study of 550 individuals with type 2 diabetes (395 in primary CV prevention, 155 in secondary CV prevention), followed at a single center after the first prescription of a GLP-1 RA between 2009 and 2019. CV and metabolic outcomes were assessed.
Results: Median duration of follow-up was 5.0 years (0.25-10.8) in primary prevention and 3.6 years (0-10.3) in secondary prevention. Median duration of treatment was 3.0 years (0-10.8). In the multivariate model, in primary prevention, duration of GLP-1 RA treatment >3 years (HR 0.19, 95% CI 0.04-0.96, P=0.044) and use of pioglitazone (HR 1.01x10-6, 95% CI 2.74x10-12-0.37, P=0.035) were associated to the risk reduction of composite outcome (MACE) which included non-fatal myocardial infarction or unstable angina, non-fatal stroke and all-cause death, while discontinuation of GLP-1 RA treatment (HR 5.58, 95% CI 1.84-16.87, P=0.034) and age (HR 1.06, 95% CI 1.01-1.11, P=0.022) were associated to higher risk. Similarly, in secondary prevention, duration of GLP-1 RA treatment >3 years (HR 0.07, 95% CI 0.01-0.30, P<0.001) turned out to be protective and conversely GLP-1 RA withdrawal (HR 4.93, 95% CI 1.37-17.69, P=0.014), was significantly associated to an increased risk of MACE. When adding hospitalizations for heart failure to the composite outcome, duration of GLP-1 RA treatment >3 years remained significant in the multivariate model in both groups.
With respect to those who withdrew treatment, subjects who continued the GLP-1 RA had significantly greater weight loss and lower glycated hemoglobin levels during follow-up.
Conclusions: In this real-world type 2 diabetes population, longer duration of GLP-1 RA treatment was associated to a reduced risk of major cardiovascular events, while medication withdrawal increased MACE risk in both subjects with and without a history of CV events.
Unveiling the paths of COVID-19 in a large city based on public transportation data
Authors: Araújo Jorge L. B. , Oliveira Erneson A. , Neto Antonio S. Lima , de Andrade José Soares , Furtado Vasco 
Published: January 2023

Abstract
Human mobility plays a key role in the dissemination of infectious diseases around the world. However, the complexity introduced by commuting patterns in the daily life of cities makes such a role unclear, especially at the intracity scale. Here, we propose a multiplex network fed with 9 months of mobility data with more than 107 million public bus validations in order to understand the relation between urban mobility and the spreading of COVID-19 within a large city, namely, Fortaleza in the northeast of Brazil. Our results suggest that the shortest bus rides in Fortaleza changed ≈ 25% more than the longest ones after an epidemic wave. Such a result is the opposite of what has been observed at the intercity scale. We also find that mobility changes among the neighborhoods are synchronous and geographically homogeneous. Furthermore, we find that the most central neighborhoods in mobility are the primary targets for infectious disease outbreaks, which is quantified here in terms of the linear relation between the disease arrival time and the average of the closeness centrality ranking. These central neighborhoods are also the top neighborhoods in the number of reported cases at the end of an epidemic wave as indicated by the exponential decay behavior of the disease arrival time in relation to the number of accumulated reported cases with decay constant λ ≈ 33 days. We believe that these results can help in the development of new strategies to impose restriction measures in the cities guiding decision-makers with smart actions in public health policies, as well as supporting future research on urban mobility and epidemiology.
Analysis of Risk Factors for the Development of Lower Extremity Deep Vein Thrombosis after Vertebral Body Fusion:A Retrospective Analysis
Authors: Ji Xinghua , Wei Jinzheng , Duan Yuchen , Liu Qingqing , Xing Zejun , Han Shufeng 
Published: January 2023

Abstract
Purpose To investigate the risk factors for lower extremity deep vein thrombosis after vertebral fusion for spinal diseases (degenerative spinal diseases, spinal trauma fractures, spinal cord injuries, excluding spinal infections and spinal tumors).
Methods The clinical data of patients aged 35 years or older who underwent vertebral fusion for spinal diseases (degenerative spinal diseases, spinal trauma fractures, spinal cord injuries, excluding spinal infections and spinal tumors) from January 2017 to August 2022 in the Department of Orthopedics, Shanxi Bethune Hospital were retrospectively analyzed. Forty-nine patients who developed DVT after surgery were included in the DVT group, and 120 patients who did not develop lower limb DVT during the same period were randomly selected in a 1:3 ratio to be included in the non-DVT group. The general data, surgery-related data, and perioperative laboratory indexes of the patients were analyzed unilaterally between groups, and the statistically significant indexes that were closely related to the occurrence of lower limb DVT after surgery were screened and included in the multifactorial logistic regression analysis.
Results The differences in BMI, surgical site, whether spinal cord injury, diabetes mellitus, trauma, preoperative muscle strength assessment, and whether preoperative D-dimer was normal were statistically significant between patients in the DVT and non-DVT groups (all P < 0.05), and multifactorial logistic regression analysis showed that spinal cord injury [OR = 3.165, 95% CI (1.187-8.439), P = 0.021], diabetes mellitus [OR = 5.191, 95% CI (1.723-15.640), P = 0.003], preoperative muscle strength grade 4 [OR = 0.072, 95% CI (0.009-0.601), P = 0.015], preoperative muscle strength grade 5 [OR = 0.036, 95% CI (0.004-0.331), P = 0.003] and preoperative D-dimer abnormalities [OR = 2.770, 95% CI (1.059-7.245), P = 0.038] were risk factors for the development of lower extremity deep vein thrombosis after vertebral fusion for degenerative spinal disease (P < 0.05).
Conclusion The occurrence of lower extremity deep vein thrombosis after vertebral fusion in elderly patients is associated with spinal cord injury, diabetes mellitus, preoperative muscle strength (≥ grade 4), and preoperative D-dimer abnormalities.
A report of radiotherapy setup errors in pelvic and mediastinal tumors
Authors: Hasoomi Nafise , Azizi Azam , Ranjbar Somayeh Amin 
Published: January 2023

Abstract
Introduction:
Radiation therapy as an important step in treatment of cancer which demands accuracy. Patient setup is a challenging job in the radiation therapy process. The Variation in random setup error for specific sites is different among clinics, even from one radiation therapist technologist to other in a same clinic. The purpose of this study is to investigate and report about the setup margins in the pelvic and mediastinal sites.
Method &Material:
34 patients (20 males and 14 females) in the supine and prone positions (24 supine positions and 10 prone positions) were selected. Internal protocol and custom-made positioning devices were utilized. The variation of Anterior-Posterior movements (AP) in daily setup is recorded based on the vertical information which is shown on the arian 2100C/D Linear Accelerators monitor. The correlation between body type, position and treatment cases compares with the mean errors.
Conclusion:
The highest random error for setup is attributed to mediastinal tumor (= 0.3 cm), and the highest systematic error is dedicated to cervix (= 1.4 cm). The population systematic error (= 1.25) is defined as the standard deviation of means of patients(Σ). In centers with lack of image-guided facilities or centers with high loaded patients the setup process should be accurate enough to limit setup error probabilities.