bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single vs. multiple operators for chronic total occlusion percutaneous coronary interventions: From the PROGRESS‐CTO Registry
Authors: Karacsonyi Judit , Alaswad Khaldoon , Krestyaninov Oleg , Karmpaliotis Dimitri , Kirtane Ajay , Ali Ziad , McEntegart Margaret , Masoumi Amirali , Poomipanit Paul , Jaffer Farouc A. , Khatri Jaikirshan , Choi James , Patel Mitul , Koutouzis Michalis , Tsiafoutis Ioannis , Gorgulu Sevket , Sheikh Abdul M. , Elbarouni Basem , Jaber Wissam , ElGuindy Ahmed , Yeh Robert , Kostantinis Spyridon , Simsek Bahadir , Rangan Bavana , Mastrodemos Olga C. , Vemmou Evangelia , Nikolakopoulos Ilias , Ungi Imre , Rafeh Nidal A. , Goktekin Omer , Burke M. Nicholas , Brilakis Emmanouil S. , Sandoval Yader 
Published: January 2023

AbstractBackgroundThere is limited data on the impact of a second attending operator on chronic total occlusion (CTO) percutaneous coronary intervention (PCI) outcomes.MethodsWe analyzed the association between multiple operators (MOs) (>1 attending operator) and procedural outcomes of 9296 CTO PCIs performed between 2012 and 2021 at 37 centers.ResultsCTO PCI was performed by a single operator (SO) in 85% of the cases and by MOs in 15%. Mean patient age was 64.4 ± 10 years and 81% were men. SO cases were more complex with higher Japan‐CTO (2.38 ± 1.29 vs. 2.28 ± 1.20, p = 0.005) and Prospective Global Registry for the Study of Chronic Total Occlusion Intervention scores (1.13 ± 1.01 vs. 0.97 ± 0.93, p < 0.001) compared with MO cases. Procedural time (131 [87, 181] vs. 112 [72, 167] min, p < 0.001), fluoroscopy time (49 [31, 76] vs. 42 [25, 68] min, p < 0.001), air kerma radiation dose (2.32 vs. 2.10, p < 0.001), and contrast volume (230 vs. 210, p < 0.001) were higher in MO cases. Cases performed by MOs and SO had similar technical (86% vs. 86%, p = 0.9) and procedural success rates (84% vs. 85%, p = 0.7), as well as major adverse complication event rates (MACE 2.17% vs. 2.42%, p = 0.6). On multivariable analyses, MOs were not associated with higher technical success or lower MACE rates.ConclusionIn a contemporary, multicenter registry, 15% of CTO PCI cases were performed by multiple operators. Despite being more complex, SO cases had lower procedural and fluoroscopy times, and similar technical and procedural success and risk of complications compared with MO cases.
Extended Tripodal Hydrotris(indazol‐1‐yl)borate Ligands as Ruthenium‐Supported Cogwheels for On‐Surface Gearing Motions
Authors: Omoto Kenichiro , Shi Menghua , Yasuhara Kazuma , Kammerer Claire , Rapenne Gwénaël 
Published: January 2023

AbstractThis paper reports the synthesis of ruthenium‐based molecular gear prototypes composed of a brominated or non‐brominated pentaphenylcyclopentadienyl ligand as an anchoring unit and a tripodal ligand with aryl‐functionalized indazoles as a rotating cogwheel. Single crystal structures of the ruthenium complexes revealed that the appended aryl groups increase the apparent diameter of the cogwheel rendering them larger than the diameter of the anchoring units and consequently making them suitable for intermolecular gearing motions once the complexes will be adsorbed on a surface.
Synthesis and Characterization of a Masked Terminal Nickel‐Oxide Complex
Authors: Heim Philipp , Spedalotto Giuseppe , Lovisari Marta , Gericke Robert , O'Brien John , Farquhar Erik R. , McDonald Aidan R. 
Published: January 2023

AbstractIn exploring terminal nickel‐oxo complexes, postulated to be the active oxidant in natural and non‐natural oxidation reactions, we report the synthesis of the pseudo‐trigonal bipyramidal NiII complexes (K)[NiII(LPh)(DMF)] (1[DMF]) and (NMe4)2[NiII(LPh)(OAc)] (1[OAc]) (LPh=2,2’,2’’‐nitrilo‐tris‐(N‐phenylacetamide); DMF=N,N‐dimethylformamide; −OAc=acetate). Both complexes were characterized using NMR, FTIR, ESI‐MS, and X‐ray crystallography, showing the LPh ligand to bind in a tetradentate fashion, together with an ancillary donor. The reaction of 1[OAc] with peroxyphenyl acetic acid (PPAA) resulted in the formation of [(LPh)NiIII−O−H⋅⋅⋅OAc]2−, 2, that displays many of the characteristics of a terminal Ni=O species. 2 was characterized by UV‐Vis, EPR, and XAS spectroscopies and ESI‐MS. 2 decayed to yield a NiII‐phenolate complex 3 (through aromatic electrophilic substitution) that was characterized by NMR, FTIR, ESI‐MS, and X‐ray crystallography. 2 was capable of hydroxylation of hydrocarbons and epoxidation of olefins, as well as oxygen atom transfer oxidation of phosphines at exceptional rates. While the oxo‐wall remains standing, this complex represents an excellent example of a masked metal‐oxide that displays all of the properties expected of the ever elusive terminal M=O beyond the oxo‐wall.
Zigzag‐Edged Polycyclic Aromatic Hydrocarbons from Benzo[m]tetraphene Precursors
Authors: Yang Bo , Gu Yanwei , Paternò Giuseppe M. , Teyssandier Joan , Maghsoumi Ali , Barker Alex J. , Mali Kunal S. , Scotognella Francesco , De Feyter Steven , Tommasini Matteo , Feng Xinliang , Narita Akimitsu , Müllen Klaus 
Published: January 2023

AbstractA series of zigzag‐edged polycyclic aromatic hydrocarbons (PAHs) (Z1‐Z3) were synthesized from 2,12‐dibromo‐7,14‐diphenyl‐benzo[m]tetraphene (9) as a versatile building block. Their structures were unambiguously confirmed by laser desorption/ionization time‐of‐flight mass spectrometry, 1H NMR, Raman, and Fourier‐transformed infrared (FTIR) spectroscopies as well as scanning tunneling microscopy. The fingerprint vibrational modes were elucidated with theoretical support. The edge‐ and size‐dependent optical properties were characterized by UV‐Vis absorption and fluorescence spectroscopy and DFT calculations. Moreover, ultrafast transient absorption spectroscopy revealed distinct modulation of the photophysical properties upon π‐extension from Z1 to Z2, the latter having a gulf edge.
A Case Study on the Use of Binding Free Energies to Screen Inhibitors of Human Carbonic Anhydrase II
Authors: Khatua Satyajit , De Bhowmik Tanusri , Taraphder Srabani 
Published: January 2023

AbstractWe present in this article a case study on the thermodynamics of binding to human carbonic anhydrase II (HCA II) by three well‐known inhibitors, viz. (a) acetazolamide (AZM) that directly binds to the catalytic Zn(II) ion at the active site, (b) non‐zinc binding 6‐hydroxy‐2‐thioxocoumarin (FC5) (c) 2‐[(S)‐benzylsulfinyl]benzoic acid (3G1). In each case, the crystal structure or its analogue of inhibitor‐bound HCA II has been used to perform classical molecular dynamics (MD) simulation in water till
. AZM and FC5 are found to undergo repeated binding and unbinding with markedly different dynamics from the partially buried, substrate‐binding hydrophobic pocket near the active site. 3G1, on the other hand, is found to remain mostly at its crystallographic binding site occluded from the active site of HCA II. The associated binding free energies (
) have been computed using the known MM/GBSA method and compared to the available experimental data. Our results show that
encounters several issues including limited sampling of multiple binding sites and incorrect prediction of the affinity of the chosen ligands. Possible use of the simulation results in further construction of Markov state models is also discussed.
Phylogenetic assessment of endangered and look‐alike Pigtoe species in a freshwater mussel diversity hotspot
Authors: Olivera‐Hyde Miluska , Jones Jess W. , Hallerman Eric M. 
Published: January 2023

AbstractThe Green River in Kentucky in the eastern United States is a freshwater mussel biodiversity hotspot, with 71 known species. Among them, the endangered Pleurobema plenum coexists with other morphologically similar species in the genera Fusconaia and Pleurobema, known colloquially as “pigtoes.” Identification of species in these genera is challenging even for mussel experts familiar with them. In our study, the correct identification of these species by experts ranged from 57% to 83%. We delineated taxonomic boundaries among seven species and tested for cryptic biodiversity among these look‐alike mussels utilizing mitochondrial and nuclear DNA sequence variation. Phylogenetic analysis of combined (1215 bp) mitochondrial DNA cytochrome oxidase I (COI) and NADH dehydrogenase 1 (ND1) genes showed five well‐diverged groups that included F. flava, F. subrotunda, P. cordatum, and P. plenum as distinct clades, with P. sintoxia and P. rubrum grouped into a single clade. While our mitochondrial DNA analyses did not distinguish P. sintoxia and P. rubrum as phylogenetically distinct species, the typical shell forms of these two nominal taxa are very distinct. Further phylogenetic analysis using nuclear ribosomal transcribed spacer region subunit I (ITS1) DNA sequences also showed that P. sintoxia and P. rubrum were not distinct lineages. No cryptic species were detected in the Fusconaia and Pleurobema samples analyzed from the Green River. The highest haplotype diversity (h), average number of nucleotide differences (k), and nucleotide diversity (π) were observed for F. subrotunda at both the COI (h = 0.896, k = 3.805, π = 0.00808) and ND1 (h = 0.984, k = 6.595, π = 0.00886) markers, with similarly high genetic diversity in the other taxa. Our results give managers confidence that cryptic taxa do not occur within or among these morphologically similar species in the Green River, and populations appear genetically diverse, indicative of large and healthy populations.
Positive impact of postfire environment on bumble bees not explained by habitat variables in a remote forested ecosystem
Authors: Johnson Sarah A. , Jackson Hanna M. , Noth Hutton , M'Gonigle Leithen K. 
Published: January 2023

AbstractBumble bees are important pollinators in temperate forested regions where fire is a driving force for habitat change, and thus understanding how these insects respond to fire is critical. Previous work has shown bees are often positively affected by the postfire environment, with burned sites supporting greater bee abundance and diversity, and increased floral resources. The extent to which fire impacts variation in bumblebee site occupancy is not well‐understood, especially in higher latitude regions with dense, primarily coniferous forests. Occupancy models are powerful tools for biodiversity analyses, as they separately estimate occupancy probability (likelihood that a species is present at a particular location) and detection probability (likelihood of observing a species when it is present). Using these models, we tested whether bumblebee site occupancy is higher in burned locations as a result of the increase in canopy openness, floral species richness, and floral abundance. We quantified the impact of fire, and associated habitat changes, on bumblebee species' occupancy in an area with high wildfire frequency in British Columbia, Canada. The burn status of a site was the only significant predictor for determining bumblebee occurrence (with burned sites having higher occupancy); floral resource availability and canopy openness only impacted detection probability (roughly, sample bias). These findings highlight the importance of controlling for the influence of habitat on species detection in pollinator studies and suggest that fire in this system changes the habitat for bumble bees in positive ways that extend beyond our measurements of differences in floral resources and canopy cover.
Non‐native hosts of an invasive seaweed holobiont have more stable microbial communities compared to native hosts in response to thermal stress
Authors: Bonthond Guido , Neu Anna‐Katrin , Bayer Till , Krueger‐Hadfield Stacy A. , Künzel Sven , Weinberger Florian 
Published: January 2023

AbstractSeaweeds are colonized by a microbial community, which can be directly linked to their performance. This community is shaped by an interplay of stochastic and deterministic processes, including mechanisms which the holobiont host deploys to manipulate its associated microbiota. The Anna Karenina principle predicts that when a holobiont is exposed to suboptimal or stressful conditions, these host mechanisms may be compromised. This leads to a relative increase of stochastic processes that may potentially result in the succession of a microbial community harmful to the host. Based on this principle, we used the variability in microbial communities (i.e., beta diversity) as a proxy for stability within the invasive holobiont Gracilaria vermiculophylla during a simulated invasion in a common garden experiment. Independent of host range, host performance declined at elevated temperature (22°C) and disease incidence and beta diversity increased. Under thermally stressful conditions, beta diversity increased more in epibiota from native populations, suggesting that epibiota from non‐native holobionts are thermally more stable. This pattern reflects an increase in deterministic processes acting on epibiota associated with non‐native hosts, which in the setting of a common garden can be assumed to originate from the host itself. Therefore, these experimental data suggest that the invasion process may have selected for hosts better able to maintain stable microbiota during stress. Future studies are needed to identify the underlying host mechanisms.
Whole‐genome resequencing reveals recent divergence of geographic populations of the dung beetle Phelotrupes auratus with color variation
Authors: Araki Yoshifumi , Sota Teiji 
Published: January 2023

AbstractKnowledge of population divergence history is key to understanding organism diversification mechanisms. The geotrupid dung beetle Phelotrupes auratus, which inhabits montane forests and exhibits three color forms (red, green, and indigo), diverged into five local populations (west/red, south/green, south/indigo, south/red, and east/red) in the Kinki District of Honshu, Japan, based on the combined interpretation of genetic cluster and color‐form data. Here, we estimated the demographic histories of these local populations using the newly assembled draft genome sequence of P. auratus and whole‐genome resequencing data obtained from each local population. Using coalescent simulation analysis, we estimated P. auratus population divergences at ca. 3800, 2100, 600, and 200 years ago, with no substantial gene flow between diverged populations, implying the existence of persistent barriers to gene flow. Notably, the last two divergence events led to three local populations with different color forms. The initial divergence may have been affected by climatic cooling around that time, and the last three divergence events may have been associated with the increasing impact of human activities. Both climatic cooling and increasing human activity may have caused habitat fragmentation and a reduction in the numbers of large mammals supplying food (dung) for P. auratus, thereby promoting the decline, segregation, and divergence of local populations. Our research demonstrates that geographic population divergence in an insect with conspicuous differences in traits such as body color may have occurred rapidly under the influence of human activity.
Implications for conservation assessment from flux in the botanical record over 20 years in southwest Ghana
Authors: Marshall Cicely A. M. , Dabo Jonathan , Mensah Markfred , Ekpe Patrick , Hawthorne William D. 
Published: January 2023

AbstractAt best, conservation decisions can only be made using the data available at the time. For plants and especially in the tropics, natural history collections remain the best available baseline information upon which to base conservation assessments, in spite of well‐documented limitations in their taxonomic, geographic, and temporal coverage. We explore the extent to which changes to the plant biological record over 20 years have changed our conception of the conservation importance of 931 plant taxa, and 114 vegetation samples, recorded in forest reserves of the southwest Ghana biodiversity hotspot. 36% of species‐level assessments changed as a result of new distribution data. 12% of species accepted in 2016 had no assessment in 1996: of those, 20% are new species publications, 60% are new records for SW Ghana, and 20% are taxonomic resolutions. Apparent species ranges have increased over time as new records are made, but new species publications are overwhelmingly of globally rare species, keeping the balance of perceived rarity in the flora constant over 20 years. Thus, in spite of considerable flux at the species record level, range size rarity scores calculated for 114 vegetation samples of the reserves in 1996 and 2016 are highly correlated with each other: r(112) = 0.84, p < .0005, and showed no difference in mean score over 20 years: paired t(113) = −0.482, p = .631. This consistency in results at the area level allows for worthwhile conservation priority setting over time, and we argue is the better course of action than taking no action at all.
Cardiovascular health metrics and risk of heart failure in a Finnish population: a prospective cohort study
Authors: Isiozor Nzechukwu M. , Kunutsor Setor K. , Voutilainen Ari , Isiozor Ifeanyichukwu , Gaye Bamba , Kauhanen Jussi , Laukkanen Jari A. 
Published: January 2023

AbstractAimsThis study aimed to examine the association between cardiovascular health (CVH) metrics and the risk of heart failure (HF) in a Finnish population.Methods and resultsWe used the prospective population‐based Kuopio Ischaemic Heart Disease cohort study comprising men aged 42–60 years at baseline (1984–89) and women aged 53–73 years at baseline (1998–2001). The CVH scores were computed from American Heart Association's CVH metrics for 2385 men and 825 women without a history of HF at baseline. The CVH scores, ranging from 0 to 14, were categorized into three: inadequate, average, and optimal groups. Multivariable Cox regression models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) for HF. During a median follow‐up period of 27 years for men, and 18 years for women, 465 and 124 HF events were recorded, respectively. The mean ages for men and women were 53 and 63 years, respectively. One hundred and thirty‐three men (5.6%) and 73 women (8.8%) had five or more ideal CVH metrics. Participants with optimal CVH scores had a 64% and 48% lower risk of HF when compared with those with inadequate CVH scores among men and women, respectively (HR, 95% CI: men = 0.36, 0.26–0.49, P < 0.01; women = 0.52, 0.31–0.89, P = 0.02).ConclusionsOptimal CVH metrics is associated with a lower risk of HF among an ageing Finnish population. Optimal CVH score should be targeted among the general population to reduce the risk of HF.
Does pain medication influence outcomes in elderly people seeking care for back pain? BACE cohort study
Authors: Zhu Z. , Chiarotto A. , Enthoven W. T. M. , Bierma‐Zeinstra S. M. A. , Koes B. W. 
Published: January 2023

AbstractBackgroundBack pain is common among older adults resulting in the high societal and economic burden of persistent pain and disability. Pain medications are frequently prescribed for back pain, especially among older patients, but the efficacy of analgesics on back pain in this patient population remains under debate. In the present study, we investigated the outcomes (i.e. pain intensity and disability) of pain medication use in older people with back pain participating in a prospective cohort study.MethodsA total of 669 patients aged >55 years consulting a general practitioner for a new episode of back complaints were included in this study. The association between pain medication use and outcomes (pain intensity and disability) was assessed at 3‐month follow‐up.ResultsPain medication users were observed to experience more pain and disability at baseline compared with non‐users. At 3‐month follow‐up, patients from all subgroups (e.g. pain medication users versus non‐users) improved over time. Yet medication users had higher pain intensity and poorer disability scores compared with non‐users. Patients who de‐escalated or stopped pain medication had lower pain and disability than non‐users. When comparing different types of medications (i.e. paracetamol, non‐steroidal anti‐inflammatory drugs, opioids), none of the groups showed better scores regarding pain and disability in users compared with non‐users. Opioid users displayed the highest levels of pain and disability.ConclusionsIn the present study, although patients who de‐escalated or stopped analgesics had less pain and disability, pain medication overall does not result in better outcomes in older people with back pain.SignificanceOlder patients taking pain medication did not report better outcomes than the ones who did not, except for those who de‐escalated or stopped pain medication. Caution should be taken in prescribing pain medication for older patients with back pain in primary care.
Real‐world data on cannabidiol treatment of various epilepsy subtypes: A retrospective, multicenter study
Authors: Kühne Fabienne , Becker Lena‐Luise , Bast Thomas , Bertsche Astrid , Borggraefe Ingo , Boßelmann Christian Malte , Fahrbach Jörg , Hertzberg Christoph , Herz Nina A. , Hirsch Martin , Holtkamp Martin , Janello Christine , Kluger Gerhard Josef , Kurlemann Gerhard , Lerche Holger , Makridis Konstantin L. , von Podewils Felix , Pringsheim Milka , Schubert‐Bast Susanne , Schulz Juliane , Schulze‐Bonhage Andreas , Steinbart David , Steinhoff Bernhard J. , Strzelczyk Adam , Syrbe Steffen , De Vries Heike , Wagner Christiane , Wagner Johanna , Wilken Bernd , Prager Christine , Klotz Kerstin A. , Kaindl Angela M. 
Published: January 2023

AbstractObjectiveCannabidiol (CBD) is approved for treatment of Dravet syndrome (DS), Lennox‐Gastaut syndrome (LGS), and tuberous sclerosis complex (TSC). Several studies suggest antiseizure effects also beyond these three epilepsy syndromes.MethodsIn a retrospective multicenter study, we analyzed the efficacy and tolerability of CBD in patients with epilepsy at 16 epilepsy centers.ResultsThe study cohort comprised 311 patients with epilepsy with a median age of 11.3 (0‐72) years (235 children and adolescents, 76 adults). Therapy with CBD was off‐label in 91.3% of cases due to age, epilepsy subtype, lack of adjunct therapy with clobazam, and/or higher dose applied. CBD titration regimens were slower than recommended, with good tolerability of higher doses particularly in children. Of all patients, 36.9% experienced a reduction in seizure frequency of >50%, independent of their epilepsy subtype or clobazam co‐medication. The median observation period was 15.8 months. About one third of all patients discontinued therapy within the observation period due to adverse effects or lack of efficacy. Adverse effects were reported frequently (46.9%).SignificanceOur study highlights that CBD has an antiseizure effect comparable to other antiseizure medications with a positive safety profile independent of the epilepsy subtype. Comedication with clobazam was not associated with a better outcome. Higher doses to achieve seizure frequency reduction were safe, particularly in children. These findings call for further trials for an extended approval of CBD for other epilepsy subtypes and for children <2 years of age.