bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analisa Unjuk Kerja Mesin Vacuum Press dengan Sistem Elektro-Pneumatik terhadap Kapasitas Produksi pada Proses Vacuum Packaging
Authors: Indrawati Ragil Tri , Pramono Agus , Yanuar Padang , Safriana Eni , Fauzi Dewa Imron , Hadiprasetya Elroy Theody , Isbar Hatta Maulana , Sadewa Leonardo Dwiky 
Published: April 2023

<p><em>Vacuum packaging</em> dilakukan guna memberikan kualitas, keamanan dan mengurangi ukuran suatu kemasan produk. Metode <em>vacuum packaging</em> dapat dilakukan secara manual maupun otomatis dengan menggunakan mesin <em>vacuum press</em>. Namun, proses <em>vacuum packaging</em> dengan cara manual menghasilkan kapasitas produksi yang rendah. Sehingga, banyak usaha yang bergerak pada industri <em>vacuum packaging</em> tidak mampu memenuhi permintaan konsumen. Pada penelitian ini dikembangkan sebuah mesin <em>vacuum </em>press dengan sistem elektro-pneumatik yang mudah dioperasikan dengan harga yang murah. Tujuan penelitian ini untuk mengetahui unjuk kerja mesin <em>vacuum </em>press dengan sistem kontrol elektro-pneumatik terhadap kapasitas produksi pada proses <em>vacuum packaging</em>. Metode penelitian menggunakan riset <em>development</em> meliputi: (1) Analisa kebutuhan berkaitan dengan spesifikasi mesin yang akan dibuat, (2) Pembuatan desain mesin <em>vacuum press</em> dengan menggunakan software <em>Computer Aided Design (CAD) </em>dan simulasi kekuatan rangka mesin menggunakan software Solidwork serta pembuatan rangkaian elektro-pneumatik yang digunakan sebagai sistem kontrol langkah kerja mesin, (3) Proses pembuatan mesin <em>vacuum press</em>, (4) Pengujian mesin <em>vacuum press</em> dan (5) Evaluasi. Penelitian ini menggunakan variabel bebas dengan waktu vakum dan variabel terikat dengan tekanan kompresor minimal 4 bar dan waktu <em>sealer</em> selama 8 detik pada saat proses pengemasan. Hasil unjuk kerja menunjukkan bahwa penggunaan mesin <em>vacuum </em>press dengan sistem kontrol elektro-pneumatik bekerja secara efektif dan efisien dibandingkan dengan proses <em>vacuum </em>konvensional. Hasil menunjukkan efektifitas cycle time sebesar 100% dan peningkatan kapasitas produksi sebesar 98.9%.</p>
In vivo characterization of sAC null sperm
Authors: Ritagliati Carla , Ayoub Sylvia , Balbach Melanie , Buck Jochen , Levin Lonny R. 
Published: April 2023

Targeted disruption of the soluble adenylyl cyclase (ADCY10; sAC) gene results in male-specific sterility without affecting spermatogenesis, mating behavior, or spermatozoa morphology and count; however, it dramatically impairs sperm motility and prevents capacitation. These phenotypes were identified in sperm from sAC null mice surgically extracted from the epididymis and studied in vitro. Epididymal sperm are dormant, and never exposed to physiological activators in semen or the female reproductive tract. To study sAC null sperm under conditions which more closely resemble natural fertilization, we explored phenotypes of ejaculated sAC null sperm in vivo post-coitally as well as ex vivo, collected from the female reproductive tract. Ex vivo ejaculated sAC null sperm behaved similarly to epididymal sAC null sperm, except with respect to the physiologically induced acrosome reaction. These studies suggest there is a sAC-independent regulation of acrosome responsiveness induced upon ejaculation or exposure to factors in the female reproductive tract. We also studied the behavior of sAC null sperm in vivo post-coitally by taking advantage of transgenes with fluorescently labelled sperm. Transgenes expressing GFP in the acrosome and DsRed2 in the mitochondria located in the midpiece of sperm (DsRed2/Acr3-EGFP) allow visualization of sperm migration through the female reproductive tract after copulation. As previously reported, sperm from wild type (WT) double transgenic mice migrated from the uterus through the uterotubular junction (UTJ) into the oviduct within an hour post-copulation. In contrast, sperm from sAC null double transgenic mice were only found in the uterus. There were no sAC null sperm in the oviduct, even 8 h after copulation. These results demonstrate that sAC KO males are infertile because their sperm do not migrate to the fertilization site.
Metagenomic next-generation sequencing and proteomics analysis in pediatric viral encephalitis and meningitis
Authors: Wang Yi-Long , Guo Xiao-Tong , Zhu Meng-Ying , Mao Yu-Chen , Xu Xue-Bin , Hua Yi , Xu Lu , Jiang Li-Hua , Zhao Cong-Ying , Zhang Xin , Sheng Guo-Xia , Jiang Pei-Fang , Yuan Zhe-Feng , Gao Feng 
Published: April 2023

IntroductionEarly and accurate identification of pathogens is essential for improved outcomes in patients with viral encephalitis (VE) and/or viral meningitis (VM).MethodsIn our research, Metagenomic next-generation sequencing (mNGS) which can identify viral pathogens unbiasedly was performed on RNA and DNA to identify potential pathogens in cerebrospinal fluid (CSF) samples from 50 pediatric patients with suspected VEs and/or VMs. Then we performed proteomics analysis on the 14 HEV-positive CSF samples and another 12 CSF samples from health controls (HCs). A supervised partial least squaresdiscriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) model was performed using proteomics data.ResultsTen viruses in 48% patients were identified and the most common pathogen was human enterovirus (HEV) Echo18. 11 proteins overlapping between the top 20 DEPs in terms of P value and FC and the top 20 proteins in PLS-DA VIP lists were acquired.DiscussionOur result showed mNGS has certain advantages on pathogens identification in VE and VM and our research established a foundation to identify diagnosis biomarker candidates of HEV-positive meningitis based on MS-based proteomics analysis, which could also contribute toward investigating the HEV-specific host response patterns.
Understanding post-surgical decline in left ventricular function in primary mitral regurgitation using regression and machine learning models
Authors: Zheng Jingyi , Li Yuexin , Billor Nedret , Ahmed Mustafa I. , Fang Yu-Hua Dean , Pat Betty , Denney Thomas S. , Dell’Italia Louis J. 
Published: April 2023

BackgroundClass I echocardiographic guidelines in primary mitral regurgitation (PMR) risks left ventricular ejection fraction (LVEF) &lt; 50% after mitral valve surgery even with pre-surgical LVEF &gt; 60%. There are no models predicting LVEF &lt; 50% after surgery in the complex interplay of increased preload and facilitated ejection in PMR using cardiac magnetic resonance (CMR).ObjectiveUse regression and machine learning models to identify a combination of CMR LV remodeling and function parameters that predict LVEF &lt; 50% after mitral valve surgery.MethodsCMR with tissue tagging was performed in 51 pre-surgery PMR patients (median CMR LVEF 64%), 49 asymptomatic (median CMR LVEF 63%), and age-matched controls (median CMR LVEF 64%). To predict post-surgery LVEF &lt; 50%, least absolute shrinkage and selection operator (LASSO), random forest (RF), extreme gradient boosting (XGBoost), and support vector machine (SVM) were developed and validated in pre-surgery PMR patients. Recursive feature elimination and LASSO reduced the number of features and model complexity. Data was split and tested 100 times and models were evaluated via stratified cross validation to avoid overfitting. The final RF model was tested in asymptomatic PMR patients to predict post-surgical LVEF &lt; 50% if they had gone to mitral valve surgery.ResultsThirteen pre-surgery PMR had LVEF &lt; 50% after mitral valve surgery. In addition to LVEF (P = 0.005) and LVESD (P = 0.13), LV sphericity index (P = 0.047) and LV mid systolic circumferential strain rate (P = 0.024) were predictors of post-surgery LVEF &lt; 50%. Using these four parameters, logistic regression achieved 77.92% classification accuracy while RF improved the accuracy to 86.17%. This final RF model was applied to asymptomatic PMR and predicted 14 (28.57%) out of 49 would have post-surgery LVEF &lt; 50% if they had mitral valve surgery.ConclusionsThese preliminary findings call for a longitudinal study to determine whether LV sphericity index and circumferential strain rate, or other combination of parameters, accurately predict post-surgical LVEF in PMR.
Experimental study of triaxial shear features of concentrated full tailings considering the effect of curing age
Authors: Wang Sha , Mei Guodong , Wang Weixiang , Wang Yali , Cui Yiyuan , Guo Lijie , Chu Yifan 
Published: April 2023

The strength evolution law of concentrated full tailings is closely related to the storage construction method, especially the growth law of shear strength with curing age. This study aims to investigate the influence of curing materials on the shear strength of full tailings and reveal the change law of the shear strength of concentrated full tailings with the curing age. Therefore, we carried out a comparative triaxial shear test between pure full tailings and concentrated full tailings with different curing ages. The test results show that concentrated full tailings had a 70.7% higher cohesion and a 10% larger internal friction angle (IFA) than that of pure full tailings at the age of 0 days. Moreover, the concentrated full tailings cured for 3 days had a 170.2% higher cohesion and a 12.1% larger IFA than that cured for 0 days. In addition, the concentrated full tailings cured for 7 days had a 149.4% higher cohesion and a 12.1% larger IFA than that cured for 0 days. The stress-strain curve exhibits that the shear strength of full tailings is significantly influenced by the curing material and curing age. The stress-strain curve gradually moved from strain hardening to strain softening as the curing age increased, and the failure curve had two steps. This indicates that the specimen still has a certain shear capacity despite having a shear zone. The entire specimen instantly fails when the strain exceeds that capacity. The results of this study can be used as scientific bases for the design and construction of the surface storage structures of concentrated full tailings.
Development of a cambrian back-arc basin in the North Qilian orogenic belt: New constraints from gabbros in Yushigou ophiolite
Authors: Tian Hao , Liu Xijun , Wu Hao , Li Dechao , Liu Xiao , Song Qi , Li Zhenglin , Liu Pengde , Hu Rongguo , Yang Qijun 
Published: April 2023

Introduction: The North Qilian orogenic belt, as the Northern branch of the original Tethys tectonic domain, is important for reconstructing the tectonic evolution of the ancient Tethys. However, the tectonic history of the North Qilian orogenic belt remains controversial. This study addresses this issue from a geochemical perspective.Methods: In this study, a comprehensive analysis of the geochronology, whole-rock geochemistry, clinopyroxene mineral geochemistry, zircon Ti crystallization temperature, and gabbromagma temperature and pressure in the Yushigou ophiolite of the North Qilian orogenic belt was conducted to provide constraints on its tectonic evolution.Results and Discussion: Laser ablation inductively coupled plasma mass spectrometry zircon U-Pb dating results reveal that the gabbros have ages of 519 ± 3 Ma and 495 ± 4 Ma, belonging to the Cambrian period. Most of the studied gabbros exhibited geochemical characteristics of tholeiitic basaltic rocks with normal mid-ocean ridge basalt and island arc tholeiite dual geochemical affinities. The gabbros are interpreted to have formed by a high degree of partial melting of the depleted mantle spinel lherzolite. These results suggest that the back-arc basin of the North Qilian tectonic belt may have evolved to a relatively mature stage from 519 to 495 Ma. Overall, this study contributes to our understanding of the tectonic evolution of the North Qilian orogenic belt through geochemical analyses.
Tutors’ chronological age and characteristics as predictors of creative nurturing behavior in the 21st-century classroom
Authors: Mahama Inuusah , Dampson Dandy George , Eshun Peter 
Published: April 2023

IntroductionThe study examined tutors’ characteristics and chronological age as they influenced their creativity nurturing behaviors.MethodsThe descriptive cross-sectional design was used to survey 340 tutors (male = 220, female = 120) from 16 colleges of education. The data for the study were gathered using adapted versions of teachers’ characteristics and creativity nurturing behaviors scales. The data were analyzed inferentially.ResultsThe study revealed that tutors’ characteristics influenced their creativity nurturing behaviors and teachers with 30 years and above of teaching could influence their creativity nurturing behaviors. Differences were established in tutors’ creativity nurturing behaviors based on their experience but such were not found in tutors’ characteristics.DiscussionIt was concluded that tutors’ characteristics and age are two key drivers of their creative abilities in the 21st century classroom. Therefore, management of teacher training institutions must cultivate an appreciable and appropriate tutor characteristics and dispositions so that they can use them to nurture trainee-teachers.
Genetic characteristics and clinical-specific survival prediction in elderly patients with gallbladder cancer: a genetic and population-based study
Authors: Zhou Hao , Chen Junhong , Jin Hengwei , Liu Kai 
Published: April 2023

BackgroundBiliary system cancers are most commonly gallbladder cancers (GBC). Elderly patients (≥ 65) were reported to suffer from an unfavorable prognosis. In this study, we analyzed the RNA-seq and clinical data of elderly GBC patients to derive the genetic characteristics and the survival-related nomograms.MethodsRNA-seq data from 14 GBC cases were collected from the Gene Expression Omnibus (GEO) database, grouped by age, and subjected to gene differential and enrichment analysis. In addition, a Weighted Gene Co-expression Network Analysis (WGCNA) was performed to determine the gene sets associated with age grouping further to characterize the gene profile of elderly GBC patients. The database of Surveillance, Epidemiology, and End Results (SEER) was searched for clinicopathological information regarding elderly GBC patients. Nomograms were constructed to predict the overall survival (OS) and cancer-specific survival (CSS) of elderly GBC patients. The predictive accuracy and capability of nomograms were evaluated through the concordance index (C-index), calibration curves, time-dependent operating characteristic curves (ROC), as well as area under the curve (AUC). Decision curve analysis (DCA) was performed to check out the clinical application value of nomograms.ResultsAmong the 14 patients with GBC, four were elderly, while the remaining ten were young. Analysis of gene differential and enrichment indicated that elderly GBC patients exhibited higher expression levels of cell cycle-related genes and lower expression levels of energy metabolism-related genes. Furthermore, the WGCNA analysis indicated that elderly GBC patients demonstrated a decrease in the expression of genes related to mitochondrial respiratory enzymes and an increase in the expression of cell cycle-related genes. 2131 elderly GBC patients were randomly allocated into the training cohort (70%) and validation cohort (30%). Our nomograms showed robust discriminative ability with a C-index of 0.717/0.747 for OS/CSS in the training cohort and 0.708/0.740 in the validation cohort. Additionally, calibration curves, AUCs, and DCA results suggested moderate predictive accuracy and superior clinical application value of our nomograms.ConclusionDiscrepancies in cell cycle signaling and metabolic disorders, especially energy metabolism, were obviously observed between elderly and young GBC patients. In addition to being predictively accurate, the nomograms of elderly GBC patients also contributed to managing and strategizing clinical care.
Identification of hub genes and biological mechanisms underlying the pathogenesis of asthenozoospermia and chronic epididymitis
Authors: Chen Yinwei , Sun Taotao , Gu Longjie , Ouyang Song , Liu Kang , Yuan Penghui , Liu Chang 
Published: April 2023

Objective: Asthenozoospermia (AZS) is one of the most common causes of male fertility, affecting family wellbeing and population growth. Chronic epididymitis (CE) is a common and lingering inflammatory disease in the scrotum. Inflammation in the epididymis has a severe impact on sperm motility. This study aimed to explore the genetic profile and critical pathways involved in the pathological mechanisms of AZS and CE, and discover potential biomarkers.Methods: Genomic datasets of AZS and CE were obtained from the Gene Expression Omnibus (GEO) database, and relevant differentially expressed genes (DEGs) were identified. GO and pathway enrichment analyses, construction of a protein-protein interaction network, and receiver operator characteristic curve analysis were conducted. The expression profile of hub genes was validated in immunohistochemical data and testicular cell data. Immune infiltration, miRNA-hub gene interactions, and gene-disease interactions were explored. The mRNA levels of hub genes were further measured by qRT-PCR.Results: A total of 109 DEGs were identified between the AZS/CE and healthy control groups. Pathways of the immune system, neutrophil degranulation, and interleukin-4 and interleukin-13 signaling were enriched in AZS and CE. Five hub genes (CD300LB, CMKLR1, CCR4, B3GALT5, and CTSK) were selected, and their diagnostic values were validated in AZS, CE, and independent validation sets (area under the curve &gt;0.7). Furthermore, the five-hub gene signature was well characterized in testicular immunohistochemical staining and testicular cells from healthy controls. Immune infiltration analysis showed that infiltration of CD8+ cells and T helper cells was significantly related to the expression level of five hub genes. In addition, a miRNA-hub gene network and interaction of other diseases were displayed. The mRNA levels of hub genes (CD300LB, CMKLR1, CCR4, and B3GALT5) were significantly elevated in the patient group. The mRNA level of CTSK also showed a similar trend.Conclusion: Our study uncovered the genetic profile involved in AZS and CE, and elucidated enriched pathways and molecular associations between hub genes and immune infiltration. This finding provides novel insight into the common pathogenesis of both diseases as well as the potential biomarkers for CE-associated AZS.
Cholera toxin B scaffolded, focused SIV V2 epitope elicits antibodies that influence the risk of SIVmac251 acquisition in macaques
Authors: Rahman Mohammad Arif , Becerra-Flores Manuel , Patskovsky Yury , Silva de Castro Isabela , Bissa Massimiliano , Basu Shraddha , Shen Xiaoying , Williams LaTonya D. , Sarkis Sarkis , N’guessan Kombo F. , LaBranche Celia , Tomaras Georgia D. , Aye Pyone Pyone , Veazey Ronald , Paquin-Proulx Dominic , Rao Mangala , Franchini Genoveffa , Cardozo Timothy 
Published: April 2023

IntroductionAn efficacious HIV vaccine will need to elicit a complex package of innate, humoral, and cellular immune responses. This complex package of responses to vaccine candidates has been studied and yielded important results, yet it has been a recurring challenge to determine the magnitude and protective effect of specific in vivo immune responses in isolation. We therefore designed a single, viral-spike-apical, epitope-focused V2 loop immunogen to reveal individual vaccine-elicited immune factors that contribute to protection against HIV/SIV.MethodWe generated a novel vaccine by incorporating the V2 loop B-cell epitope in the cholera toxin B (CTB) scaffold and compared two new immunization regimens to a historically protective ‘standard’ vaccine regimen (SVR) consisting of 2xDNA prime boosted with 2xALVAC-SIV and 1xΔV1gp120. We immunized a cohort of macaques with 5xCTB-V2c vaccine+alum intramuscularly simultaneously with topical intrarectal vaccination of CTB-V2c vaccine without alum (5xCTB-V2/alum). In a second group, we tested a modified version of the SVR consisting of 2xDNA prime and boosted with 1xALVAC-SIV and 2xALVAC-SIV+CTB-V2/alum, (DA/CTB-V2c/alum).ResultsIn the absence of any other anti-viral antibodies, V2c epitope was highly immunogenic when incorporated in the CTB scaffold and generated highly functional anti-V2c antibodies in the vaccinated animals. 5xCTB-V2c/alum vaccination mediated non-neutralizing ADCC activity and efferocytosis, but produced low avidity, trogocytosis, and no neutralization of tier 1 virus. Furthermore, DA/CTB-V2c/alum vaccination also generated lower total ADCC activity, avidity, and neutralization compared to the SVR. These data suggest that the ΔV1gp120 boost in the SVR yielded more favorable immune responses than its CTB-V2c counterpart. Vaccination with the SVR generates CCR5- α4β7+CD4+ Th1, Th2, and Th17 cells, which are less likely to be infected by SIV/HIV and likely contributed to the protection afforded in this regimen. The 5xCTB-V2c/alum regimen likewise elicited higher circulating CCR5- α4β7+ CD4+ T cells and mucosal α4β7+ CD4+ T cells compared to the DA/CTB-V2c/alum regimen, whereas the first cell type was associated with reduced risk of viral acquisition.ConclusionTaken together, these data suggest that individual viral spike B-cell epitopes can be highly immunogenic and functional as isolated immunogens, although they might not be sufficient on their own to provide full protection against HIV/SIV infection.
Effect of dietary bile acids supplementation on growth performance, feed utilization, intestinal digestive enzyme activity and fatty acid transporters gene expression in juvenile leopard coral grouper (Plectropomus leopardus)
Authors: Gao Yujie , Yao Yifan , Huang Jie , Sun Yuejia , Wu Qingjun , Guo Dingqian , Wang Shengpeng 
Published: April 2023

This study aimed to evaluate the effects of dietary bile acids on growth performance, feed utilization, body composition, digestive enzyme activity, and related gene expression in juvenile leopard coral grouper (Plectropomus leopardus). Five diets with varying levels of dietary bile acids (0, 0.15, 0.3, 0.45 and 0.6%) were formulated and each diet was fed to triplicate groups of 15 fish (13.14 ± 0.14 g) for ten weeks. Results showed that the significant enhancement in weight gain (WG) and specific growth rate (SGR) was observed in fish fed 0.3% bile acids (P &lt; 0.05). Additionally, feed conversion ratio (FCR) was significantly improved with bile acids inclusion (P &lt; 0.05). Higher feed intake was also recorded in bile acids inclusion groups, and significantly up-regulated gene expression of hypothalamus neuropeptide Y (NPY) was found in 0.3% group compared with the control group (P &lt; 0.05). Dietary bile acids (0.3%) significantly decreased lipid deposition in the whole-body (P &lt; 0.05), and lower hepatosomatic index (HSI) and viscerosomatic index (VSI) were also found in this group. Moreover, intestinal trypsin and lipase activities were significantly increased in 0.3% group (P &lt; 0.05) to promote feed digestion. In addition, the relative expression levels of intestinal fatty acid binding protein 2 (FABP2) significantly up-regulated with inclusion of dietary bile acids, suggesting an enhancement of fatty acid transport (P &lt; 0.05). In contrast, cholesterol transport related genes NPC1 like intracellular cholesterol transporter 1 (NPC1L1) and CD36 were notably down-regulated as bile acids inclusion (P &lt; 0.05). This study provides valuable information for the formulation of diets for juvenile coral trout and indicates that dietary bile acids could potentially be used to improve growth performance and feed utilization.