bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of cognition in combination with an active cycle of breathing technique on dyspnea-related kinesiophobia in patients with moderate to severe chronic obstructive pulmonary disease
Authors: CAI Shasha , YAO Jinlan , HAN Maomao , LUO Xiaolin , YU Yudi , LU Xiaorong , XIANG Xinyue , HUANG Lihua 
Published: January 2023

Abstract
Background

The majority of patients with chronic obstructive pulmonary disease (COPD) have a catastrophic perception of dyspnea and avoid physical activity, with moderate to severe COPD patients exhibiting more pronounced kinesiophobia. The fear-avoidance model suggests that kinesiophobia can be alleviated by reducing dyspnea symptoms and altering the catastrophic perception of dyspnea as an illness. However, we discovered that single inspiratory muscle training and cognitive change have limitations. This study combined cognition and exercise to examine the efficacy of cognitive therapy based on social cognitive theory combined with an active cycle of breathing technique in reducing dyspnea-related kinesiophobia in patients with moderate to severe COPD.

Methods

This research involved 106 participants. The control group (N = 53) got standard nursing, whereas the intervention group (N = 53) received cognitive combined with active cycle of breathing technology intervention for 8 weeks. Lung function, the breathlessness beliefs questionnaire, and other secondary outcomes were measured before and after the intervention. The breathlessness beliefs questionnaire was administered one, two and three months following the intervention. This study's statistical analysis was conducted using SPSS 22.0 software.

Results

The between-group effects, within-group effects, and interaction effect of BBQ, BBQ-SF, and BBQ-AA scores in the intervention group were statistically significant (P<0.05) when compared to the control group. At the conclusion of the 8-week intervention, the intervention group's FVC, FEV1, and 6-minute walk distance were considerably greater than the control group's, while the intervention group's mMRC, BODE index, and CAT score were significantly lower (P<0.05). However, no significant change in FEV1% or FEV1/FVC was seen between the two groups (P > 0.05).

Conclusion

The misconception of dyspnea in COPD patients served as the starting point for this study, which integrated cognitive and exercise training. The combined intervention substantially improved dyspnea-related kinesiophobia in patients with moderate to severe COPD, and the effect remained significant when the intervention duration was extended. Furthermore, it may enhance dyspnea and quality of life, increase exercise capacity, and lower the BODE index.

Trial registration:

Code: ChiCTR2200058498, Date: 10/04/2022, http://www.chictr.org.cn/showproj.aspx?proj=164225
Psychometric Properties of the Environmental Factors’ Questionnaire for Undergraduate Medical Students Taking Online Learning during COVID 19 Pandemic: A cross-sectional study
Authors: Taleb Ola K. , Hamid Siti Azrin Ab , Abdullah Sarimah , Abusafia Ali H. , Baharuddin Kamarul Aryffin , Adnan Wan Nor Asyikeen Wan 
Published: January 2023

Abstract
Background
Environmental factors are important for students learning during online classes, especially during COVID-19. This study aimed to validate the environmental factors’ questionnaire during online learning.
Methods
A total of 218 undergraduate medical students at the Health Campus, Universiti Sains Malaysia participated in a cross-sectional study that involved an online survey. Environmental factors scales were assessed with the 9-items Lighting, Noise and Temperature (LNT) scale and the 6-items Technology scale. Analysis was performed using Confirmatory Factor Analysis (CFA).
Results
The English version of LNT with nine items and three factors showed a good fit to the data with no item deleted. For lighting, noise and temperature, the Composite Reliability (CR) was 0.81, 0.81 and 0.84, respectively while the Average Variance Extracted (AVE) was 0.61, 0.59 and 0.6, respectively. The English version of the Technology scale with six items and one factor also showed a good fit to the data with no item deleted. The CR was 0.84, and AVE was 0.51.
Conclusions
The results give psychometric evidence for environmental questionnaire scales in evaluatingthe factors associated with online learning among Malaysian university medical students. All items were retained and confirmed to be fit for sample data.
Node importance evaluation in multi-platform avionics architecture based on TOPSIS and PageRank
Authors: Chang Liu , JinYan Wang , Rui Xia 
Published: January 2023

Abstract
In this paper, a node importance evaluation method for multi-platform avionics architecture oriented to collaborative task capability is proposed, aiming at the evaluation problem of node contribution under the air combat collaborator, focusing on the influence brought by collaborative task information activities, considering the charged relationship between nodes, link networking relationship, collaborative task style relationship, data information processing, storage mode, and other factors. The evaluation results mainly reflect the contribution and importance of nodes to collaborative tasks. First, the TOPSIS method was used to evaluate the overall contribution of nodes under different indicator scales. 2) Secondly, a PageRank evaluation method is proposed to reflect the node-pointing relationship. The comprehensive weight of nodes considers the rationality of TOPSIS and PageRank algorithms. 3) Finally, the information entropy method is used to evaluate the overall combat capability.
FBXW7 inhibits the Progression of ESCC by directly inhibiting the Stemness of Tumor Cells
Authors: Yang Yanfang , Bi Yanghui , Cheng Caixia , Tang Peisen , Xiao Heng , Yuan Fajia , Wu Weiwei , Yang Bin 
Published: January 2023

Abstract
Background
F-box and WD repeat domain containing 7 (FBXW7), is an aboriginal and high frequency mutant gene associated with esophageal squamous cell carcinoma (ESCC). This study was designed to determine the clinical value and molecular mechanisms of FBXW7 in the development of ESCC.
Methods
The clinical significance of FBXW7 was analyzed in ESCC from TCGA data. The effects of FBXW7 on proliferation, colony formation, migration and invasion, angiogenesis and apoptosis were tested in ESCC cells. PCR-array, sphere formation assay, quantitative real-time polymerase chain reaction(qPCR) were used to explore the mechanism of FBXW7.
Results
FBXW7 was a significantly mutated gene in ESCC. It was an independent and potential predictor for survival in ESCC patients. In addition, FBXW7 overexpression significantly inhibited ESCC cell proliferation, migration, invasion, angiogenesis, and promoted cell apoptosis. PCR-array revealed that FBXW7 overexpression leads to a significant change of genes expression associated with angiogenesis, cell senescence and DNA damage and repair. Sphere formation assay and qPCR showed FBXW7 was associated with ESCC stem cell formation.
Conclusions
Our results suggest that FBXW7 may act as a tumor suppressor by repressing cancer stem cell formation and regulating tumor angiogenesis, cell senescence, DNA damage and repair in ESCC.
The Effect of Recombinant Fusion Peptide Capsular Biosynthesizing Enzymes A, C Streptococcus agalactiae and Anti-Cancer Peptide against Cell Line Hela by Real-time RT- PCR and Flow cytometry
Authors: Babakanrad Elmira , Mohammadian Taher , Esmaeili Davoud , Behzadi Payam 
Published: January 2023

Abstract
There have been numerous reports of the effect of the Streptococcus agalactiae peptide and its capsule products on cervical cancer. This study aimed to investigate the inhibitory effect of the recombinant anti-cancer protein CpsA-CpsC-L-ACAN on the HeLa cell. The CpsA-CpsC-L-ACAN sequence construction was obtained from NCBI. The Structure of CpsA-CpsC-L-ACAN was examined using various bioinformatics software programs. After creating and cloning into the expression vector pET-22b (+), CpsA-CpsC-L-ACAN was transferred to E. coli BL21(DE3). Nickel column chromatography and Western blotting were used to perform purification and confirmation of CpsA-CpsC-L-ACAN. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) approach was utilized for investigating the cell-killing effect of different concentrations of CpsA-CpsC-L-ACAN against the HeLa cell. Moreover, using Real-time reverse transcription polymerase chain reaction (Real-time RT-PCR), the expression of apoptotic genes, like Bcl-2, caspase-3, and Bax before and after exposure to CpsA-CpsC-L-ACAN was measured. Then, CpsA-CpsC-L-ACAN impact on HeLa cells was examined by flow cytometry. The antibacterial properties of the CpsA-CpsC-L-ACAN were evaluated by the minimum inhibitory concentration (MIC) test and the disk diffusion test. CpsA-CpsC-L-ACAN at a concentration of 64µg/ml killed 50% of cancer cells in 24 hours and after the treatment of Hela cells with CpsA-CpsC-L-ACAN protein, the expression of apoptosis genes, caspase-3 and Bax increased 16 and 6 times, respectively. Also, the expression of bcl-2 by 0.176 times decreased. According to the results of the flow cytometry test, after treatment with CpsA-CpsC-L-ACAN, the cancer cell population transitioned from the living phase to the apoptotic phase.
Optical coherence tomography angiography features in patients with idiopathic macular holes before and after vitrectomy
Authors: Qi Xuemin , Gao Fengjie , Gao Xiaoming , Liu Hongfei 
Published: January 2023

Abstract
Purpose
Optical coherence tomography angiography (OCTA) is a novel noninvasive imaging tool for retinal and optic nerve head (ONH) perfusion. The study aimed to explore OCTA features in patients with idiopathic macular holes (IMHs) before and after vitrectomy.
Methods
The retrospective case series study included patients with IMHs diagnosed by OCT between June 2019 and June 2021 in our hospital. The best corrected visual acuity (BCVA), superficial and deep foveal avascular zone (FAZ) area, blood flow density within 300µm width of FAZ (FD300) and the state of external limiting membrane (ELM) were examined by OCTA.
Results
Totally 26 patients (9 men and 17 women) aged 65.08 ± 5.09 years were included, with an average disease course of 154.15 ± 206.26 days. Visual acuity showed no improvement at 1 month but was improved at 6 months (P < 0.05). FAZ, superficial FAZ, deep FAZ, and FD300 showed a decreasing tendency at 1 month, indicating an amelioration following surgery. Though data within 6 months showed decreased efficacy over time, there were significant improvements compared with preoperative parameters (P < 0.05). The 1-month FAZ in the deep layer (P < 0.01) and FD300 (P < 0.01) were significantly increased in the ELM (+) group. The differences were more pronounced at 6 months (FAZ in the deep layer: P < 0.01; FD300: P < 0.01).
Conclusion
OCTA may dynamically monitor the neurovascular repair process of IMH and predict the prognosis of visual function after IMH operation.
Translation into Spanish and cross-cultural adaptation and validation of the Problem Areas in Diabetes – Pediatric version (PAID-Peds) survey: study protocol
Authors: Casanovas-Marsal Josep-Oriol , Monzón Elisa Civitani , Duce Maria-Pilar Ferrer , Cuesta Delia González de la , Valverde Rosa Yelmo , Repiso Victoria Pérez , Manterola Irune Goicoechea , Muñoz Antonio de Arriba 
Published: January 2023

Abstract
Background
The metabolic and psychological management of pediatric type 1 diabetes mellitus (T1DM) can be challenging over time given that T1DM may cause a negative emotional burden and, consequently, result in poor metabolic control of the disease. The objectives of this study are to translate the Problem Area in Diabetes Survey–Pediatric version (PAID-Peds) into Spanish, adapt it culturally and validate it.
Methods
A multicenter, observational, prospective and analytical study based on a sample of 636 patients aged 8–17 years, diagnosed with T1DM, under treatment with insulin and follow-up at the Miguel Servet University Hospital in Zaragoza (Aragón, Spain), the Ramón y Cajal University Clinical Hospital in Madrid (Spain) and at the Sant Joan de Déu Hospital in Barcelona (Catalonia, Spain) between 1 January 2023 and 31 December 2024.
The study will consist of two phases: 1) Translation and cultural adaptation of the original PAID-Peds® survey into Spanish following eight steps; 2) Validation of the Spanish version of the PAIS-Peds® survey. The statistical analysis will be performed using Jamovi® 2.1.23, the reliability or internal consistency will be calculated using Cronbach’s alpha index (considering an index higher than 0.8 to be good) and the test-retest will be evaluated using the intraclass correlation coefficient. This study has been approved by the ethics and research committees at each center.
Discussion
Detecting the burden of having diabetes could favor early interventions to reduce anxiety and prevent the worsening of distress, exhaustion, and depressive symptoms, and subsequent poor diabetes control. Therapeutic education in diabetes—recommended by the WHO and the Diabetes Education Study Group—has shown encouraging results in glycemia and psychosocial and behavioral factors in T1DM.
Evaluation of colonic anastomosis healing using hybrid nanosheets containing Molybdenum disulfide (MOS2) scaffold of human placental amniotic membrane and Polycaprolactone (PCL) In rat animal model
Authors: Soltani Zahra Ebrahim , Elahi Mohammad , Tashak-Golroudbari Hasti , Nazari Hojjatollah , Heirani Asieh , Asbagh Reza Akbari , Ohadi Mohammad Amin Dabbagh , shabani Moojan , Sarzaeim Mojdeh , Kazemeini Alireza , Keramati Mohammad Reza , Behboudi Behnam , Tafti Seyed Mohsen Ahmadi 
Published: January 2023

Abstract
Purpose
Anastomosis is a standard technique following different conditions, such as obstruction, tumor, and trauma. Obstruction, adhesion, or anastomosis leakage can be some of its complications. To improve healing and prevent postoperative complications, we design a hybrid scaffold containing acellular human Amniotic membranes and Polycaprolactone-Molybdenum disulfide nanosheets for colon anastomosis.
Methods
The animal model of colocolonic anastomosis was performed on two groups of rats; control and scaffold. The hybrid scaffold was warped around the anastomosis site in the scaffold group. Samples from the anastomosis site were resected on the third and seventh postoperative days for histopathological and molecular assessment.
Results
Histopathologic score and burst pressure had shown significant improvement in the scaffold group. No mortality and anastomosis leakage was reported in the scaffold group. In addition, inflammatory markers were significantly decreased, while anti-inflammatory cytokines were increased in the scaffold group.
Conclusion
The result indicates that our hybrid scaffold is a proper choice for colorectal anastomosis repair by declining postoperative complications and accelerating healing.
Folic Acid Deficiency in severe hypothyroidism: a Case Report and a Review of Literature
Authors: Adan Abdisamad Mohamed , Siyad Mohamed Osman , Jeele Mohamed Osman Omar 
Published: January 2023

Abstract
Folate deficiency can be caused by an insufficient diet, conditions that impede folate absorption, certain medications, and congenital problems. It may also be resulted from alcoholism, pregnancy, breastfeeding, hemolytic anemia, and dialysis. According to the European prevalence estimates, hypothyroidism affects up to 5% of the population, while up to 5% of the community may very well have unrecognized thyroid insufficiency. Although, it’s widely accepted that the most common type of anemia in hypothyroidism patients is normocytic normochromic anemia as reported by various studies, macrocytic anemia in case of folic acid deficiency can also occur. Folic acid deficiency can present with a newly diagnosed hypothyroidism as in our case. Despite the relationship between hypothyroidism and folic acid deficiency, it has not been studied deservedly and the case reports with this combination are scarce in the literature. In this case report, we present a case of 22-year- old woman who presented with folic acid deficiency anemia and hypothyroidism. The patient symptoms improved after treatment with folic acid and thyroid replacement therapy.
Selection signatures and population dynamics of transposable elements in Lima bean
Authors: Lozano-Arce Daniela , Garcia Tatiana , Gonzalez-Garcia Laura , Guyot Romain , Sanchez Maria , Duitama Jorge 
Published: January 2023

Abstract
The domestication process in Lima bean (Phaseolus lunatus L.) involves at least two independent events, within the Mesoamerican and Andean gene pools. Both processes produced similar phenotypic changes in landraces, making Lima bean an excellent model to understand convergent evolution. Despite recent research efforts, the mechanisms of adaptation followed by Mesoamerican and Andean landraces are largely unknown. The genes related to these adaptations can be selected by identification of selective sweeps within gene pools. Most of the previous genetic analyses in Lima bean have relied on Single Nucleotide Polymorphism (SNP) loci and have ignored transposable elements (TEs) which are a major source of variation in plant genomes. The current availability of high-throughput sequencing technologies enables the collection of whole-genome resequencing (WGS) data to approach intraspecies population dynamics of TEs. The present research collected WGS data from 60 wild and domesticated Lima bean accessions to generate the most complete characterization developed to date of transposable elements and SNP loci in the Lima bean genome. We generated an updated annotation of 223,780 transposable elements in the Lima bean genome. Furthermore, we identified genes and variable TEs affected by selective sweeps. Combining three different approaches, selective sweeps were predicted to generate a set of domestication candidate genes. A small percentage of genes under selection (1.6%) were shared among gene pools, suggesting that domestication followed different genetic avenues in both gene pools. Up to 25% of the genes with previously reported selective sweeps in common bean were also detected in Lima bean. We also built a catalog of 39,459 TEs with presence-absence variation (PAV). The fact that 75% of these TEs were located close to genes shows their potential to affect gene functions in Lima bean. The genetic structure inferred from variable TEs was consistent with that obtained from SNP markers, suggesting that TE dynamics can be related to the demographic history of wild and domesticated Lima bean and its adaptive processes, in particular selection processes during domestication.
Predicting Radiation Esophagitis using 18F-FAPI-04 PET/CT in Patients with LA-ESCC Treated with Concurrent Chemoradiotherapy
Authors: Hu Xinying , Han Xiao , Zhang Mingquan , Mu Zhengshuai , Fu Zheng , Ren Jiazhong , Qiao Kailin , Jia Jing , Yu Jinming , Yuan Shuanghu , Wei Yuchun 
Published: January 2023

Abstract
Purpose:
This prospective study examined whether 18F-FAPI-04 PET/CT can predict the development and severity of radiation esophagitis (RE) in patients with locally advanced esophageal squamous cell carcinoma (LA-ESCC) treated with concurrent chemoradiotherapy.
Methods:
From June 2021 to March 2022, images were prospectively collected from LA-ESCC patients who underwent 18F-FAPI-04 PET/CT examinations before and during radiotherapy. The development of RE was evaluated weekly according to Radiation Therapy Oncology Group criterion. The target-to-background ratio in blood (TBRblood) was analyzed at each time point and correlated with the onset and severity of RE. Factors that predicted RE were identified by multivariate logistic analyses.
Results:
Thirty patients (median age, 66.5 years [interquartile range: 56–71 years]; 22 men) were evaluated. Significantly higher TBRblood (during radiotherapy, mean: 3.06 vs 7.11, P = 0.003) and change in TBRblood compared with pre-RT (ΔTBRblood, mean: 0.67 vs 4.81, P = 0.002) were observed in patients with RE than patients without RE. Those with grade 3 RE had a significantly higher TBRblood (during radiotherapy, mean: 4.55 vs 9.66, P = 0.003) and ΔTBRblood (mean: 2.16 vs 7.50, P = 0.003) compared with those with RE < grade 3. Receiver operating characteristic curve analysis identified TBRblood (during radiotherapy, AUC = 0.902; AUC = 0.912) and ΔTBRblood (AUC = 0.911; AUC = 0.922) as significant predictors of the development of RE and specifically grade 3 RE. On multivariate analysis, ΔTBRblood was identified as a significant predictor of both RE (P = 0.021) and grade 3 RE (P = 0.038).
Conclusion:
The ΔTBRblood on 18F-FAPI-04 PET/CT may be effective at identifying patients at risk for the development of RE, especially grade 3 RE.
Interrater agreement between student and teacher assessments of endotracheal intubation skills in a self-directed simulation learning environment
Authors: Mankute Aida , Juozapaviciene Laima , Stucinskas Justinas , Dambrauskas Zilvinas , Dobozinskas Paulius , Sinz Elizabeth , Rodgers David L. , Pukenyte Evelina , Kumpaitiene Birute , Vaitkaitis Dinas 
Published: January 2023

Abstract
Background: Knowledge assessment is an important part of the learning process to confirm competencies in acquired medical knowledge.
Objective: This study aimed to compare the assessments of endotracheal intubation skills using the HybridLab® methodology between students and teachers in terms of interobserver reliability.
Methods: Reliability analysis was performed with observational data (data are reported according to STROBE guidelines). The study was conducted in two countries, the Lithuanian University of Health Science (LUHS) and Pennsylvania State University (PSU) in the US, between 1 January and 30 June 2020. A total of 92 students (60 from LUHS and 32 from PSU) were trained in endotracheal intubation using an algorithm-driven hybrid learning method. At the end of the training session, the participants had to complete the evaluation scenario, which was assessed by one of the students and evaluated remotely by a single teacher. The student assessment of the endotracheal intubation procedure was compared with the teacher’s assessment using correlation and estimation of the intraclass correlation coefficient.
Results: Overall, the medians of the student and teacher assessments were both 100% (0%). Spearman’s correlation coefficient between the student and teacher assessments was 0.879 (p=0.001). The intraclass correlation coefficient used for interobserver variations between the students and teachers was 0.883 (95% confidence interval from 0.824 to 0.923).
Conclusions: The algorithm-driven hybrid learning method allows students to reliably assess endotracheal intubation skills to a level comparable with that of the teacher’s evaluation.
Synthesis of Silver nanoparticles using bioactive seaweeds against poultry pathogens
Authors: Sivakumar S. R. 
Published: January 2023

Abstract
There is an increasing demand to create nanomaterials that are eco-friendly, non-toxic, and less expensive in relation to human health. In order to investigate the effectiveness of the marine macro algae silver nanoparticles against two specific poultry bacterial infections, methanol extracts of Halimeda macroloba (green), Turbinaria conoides (brown), and Spyridia filamentosa (red) were used. The zone of inhibition against the two bacterial pathogens as well as AgNO3 as a control increases with an increase in the concentration of marine macro algae synthesized nanoparticles. The characterization by UV, FTIR, XRD, SEM and EDAX reveals the formation of silver nanoparticle through seaweeds and the LCMS data reveals the presence of bioactive compounds which exhibit antibacterial activity. Thus the chemical compounds such as phyllophenone and phorbasterone present in the marine macro algae may be responsible for the antibacterial action of poultry pathogens such as Staphylococcus aureus and Salmonella typhi according to LC-MS characterizations.