bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of bihemispheric transcranial direct current stimulation on distal upper limb function and corticospinal tract excitability in a patient with subacute stroke: a case study
Authors: Shiba Takahiro , Mizuta Naomichi , Hasui Naruhito , Kominami Yohei , Nakatani Tomoki , Taguchi Junji , Morioka Shu 
Published: September 2023

IntroductionActivation of the unaffected hemisphere contributes to motor function recovery post stroke in patients with severe upper limb motor paralysis. Transcranial direct current stimulation (tDCS) has been used in stroke rehabilitation to increase the excitability of motor-related areas. tDCS has been reported to improve upper limb motor function; nonetheless, its effects on corticospinal tract excitability and muscle activity patterns during upper limb exercise remain unclear. Additionally, it is unclear whether simultaneously applied bihemispheric tDCS is more effective than anodal tDCS, which stimulates only one hemisphere. This study examined the effects of bihemispheric tDCS training on corticospinal tract excitability and muscle activity patterns during upper limb movements in a patient with subacute stroke.MethodsIn this single-case retrospective study, the Fugl–Meyer Assessment, Box and Block Test, electromyography, and intermuscular coherence measurement were performed. Intermuscular coherence was calculated at 15–30 Hz, which reflects corticospinal tract excitability.ResultsThe results indicated that bihemispheric tDCS improved the Fugl–Meyer Assessment, Box and Block Test, co-contraction, and intermuscular coherence results, as compared with anodal tDCS. Discussion: These results reveal that upper limb training with bihemispheric tDCS improves corticospinal tract excitability and muscle activity patterns in patients with subacute stroke.
Soil carbon plays a role in the climate impact of diet and its mitigation: the Finnish case
Authors: Saarinen Merja , Heikkinen Jaakko , Ketoja Elise , Kyttä Venla , Hartikainen Hanna , Silvennoinen Kirsi , Valsta Liisa , Lång Kristiina 
Published: September 2023

IntroductionDiet has a significant impact on the consumer’s climate impact, and a radical global change in the food system is necessary. However, the change needs to be interpreted and adapted to local conditions.MethodsTo support national climate policy, we evaluated current Finnish diet and its four alternatives: “current diet”; “meat to half diet”; “meat to a third diet”; “a diet rich in fish and milk”; and “a vegan diet”. We created the FoodMin model to simultaneously address both climate impacts and nutrient uptake and to combine carbon dioxide (CO2) emissions from soil organic matter (SOM) degradation with dietary climate impact assessments. As a well-established assessment method remains lacking, product group-specific estimates for the CO2 emissions of SOM origin were produced in two different ways, based on long-term observational data or by modelling with the Yasso07. We also examined, using three scenarios, how much the achievement of soil carbon (C) balance in Finnish production could affect the climate impact of the diet.Results and DiscussionThe climate impact of the current diet was 6.0 kg CO2 per person per day, and for alternative diets, the change compared with the current diet was −14%, −20%, −31%, and −39% respectively, for “meat to half” (5.2 kg CO2 eq. per person per day), “meat to third” (4.8), “a diet rich in fish and milk” (4.2), and “the vegan diet” (3.7). SOM-derived CO2 emissions from domestic fields accounted for 18% of the climate impact of the current diet and up to 23% of alternative diets. In terms of the soil C-balance scenarios, all actions together could mean a 3–13% reduction in the climate impact of the diet: the more products of animal origin, the more reduction opportunities in the diet. With the change in diet, these measures could reduce the climate impact of diets by 13–41%. The nutritional value of a “diet rich in fish and milk” was the best. The study revealed that SOM-induced CO2 emissions and SOM-related activities play a very important role in the climate impact of the diet and its mitigation; they cannot be ignored in dietary assessments in addition to direct product choices.
Effects of nano-selenium on cecum microbial community and metabolomics in chickens challenged with Ochratoxin A
Authors: Fang Manxin , Hu Wei , Liu Ben 
Published: September 2023

IntroductionOchratoxin A (OTA) is a widely distributed mycotoxin. Nano-selenium (Nano-Se) is an emerging form of selenium known for its superior bioavailability, remarkable catalytic efficiency, and robust adsorbing capacity. Despite these characteristics, its impact on the microbial community and metabolomics in the cecum of chickens exposed to OTA has been infrequently investigated. This research examined the microbiota and metabolomic alterations linked to OTA in chickens, with or without Nano-Se present.MethodsA cohort of 80 healthy chickens at the age of 1 day was randomly distributed into four groups of equal numbers, namely the Se cohort (1 mg/kg Nano-Se), the OTA cohort (50 μg/kg OTA), the OTA-Se cohort (50 μg/kg OTA + 1 mg/kg Nano-Se), and the control group. Each chicken group’s caecal microbiome and metabolome were characterized using 16S rRNA sequencing and Liquid chromatography coupled with mass spectrometry (LC–MS) analyses.Results and discussionOur results showed that the on day 21, the final body weight was significantly reduced in response to OTA treatments (p &lt; 0.05), the average daily gain in the OTA group was found to be inferior to the other groups (p &lt; 0.01). In addition, Nano-Se supplementation could reduce the jejunum and liver pathological injuries caused by OTA exposure. The 16S rRNA sequencing suggest that Nano-Se supplementation in OTA-exposed chickens mitigated gut microbiota imbalances by promoting beneficial microbiota and suppressing detrimental bacteria. Moreover, untargeted metabolomics revealed a significant difference in caecal metabolites by Nano-Se pretreatment. Collectively, the dataset outcomes highlighted that Nano-Se augmentation regulates intestinal microbiota and associated metabolite profiles, thus influencing critical metabolic pathways, and points to a possible food-additive product.
Yeast Lsm Pro-Apoptotic Mutants Show Defects in Autophagy
Authors: Caraba Benedetta , Stirpe Mariarita , Palermo Vanessa , Vaccher Ugo , Bianchi Michele Maria , Falcone Claudio , Mazzoni Cristina 
Published: September 2023

LSM4 is an essential yeast gene encoding a component of different LSM complexes involved in the regulation of mRNA splicing, stability, and translation. In previous papers, we reported that the expression in S. cerevisiae of the K. lactis LSM4 gene lacking the C-terminal Q/N-rich domain in an Lsm4 null strain S. cerevisiae (Sclsm4Δ1) restored cell viability. Nevertheless, in this transformed strain, we observed some phenotypes that are typical markers of regulated cell death, reactive oxygen species (ROS), and oxidated RNA accumulation. In this paper, we report that a similar truncation operated in the S. cerevisiae LSM4 gene confers on cells the same phenotypes observed with the K. lactis lsm4Δ1 gene. Up until now, there was no evidence of the direct involvement of LSM4 in autophagy. Here we found that the Sclsm4Δ1 mutant showed a block in the autophagic process and was very sensitive to nitrogen starvation or treatment with low doses of rapamycin, an inducer of autophagy. Moreover, both during nitrogen starvation and aging, the Sclsm4Δ1 mutant accumulated cytoplasmic autophagy-related structures, suggesting a role of Lsm4 in a later step of the autophagy process.
Risk Factors Associated with Failure and Technical Complications of Implant-Supported Single Crowns: A Retrospective Study
Authors: Larsson Adam , Manuh Justice , Chrcanovic Bruno Ramos 
Published: September 2023

Background and Objectives: Implant-supported single crowns have become a routine approach for the replacement of missing single teeth, being considered as one of the most common ways of rehabilitation when adjacent teeth are healthy. The present retrospective study aimed to investigate the risk factors possibly associated with failure and technical complications of implant-supported single crowns and their supporting implants. Materials and Methods: Patients treated at one faculty (2009–2019) were considered for inclusion. Complications investigated included ceramic fracture/chipping, crown loss of retention/mobility, crown failure/fracture, loosening/loss/fracture of prosthetic screw, and implant failure/fracture. Any condition/situation that led to the removal/replacement of crowns (implant failure not included) was considered prosthesis failure. Univariate/multivariate Cox regression models were used to evaluate the associations between clinical covariates and failure. Results: 278 patients (358 crowns) were included. Mean ± SD follow-up was 56.5 ± 29.7 months. Seven implants (after a mean of 76.5 ± 43.7 months) and twenty crowns (21.3 ± 23.5 months) failed. The cumulative survival rate (CSR) for crowns was 93.5% after 5, remaining at 92.2% between 6 and 11 years. The most common reasons for crown failure were porcelain large fracture (n = 6), crown repeatedly loose (n = 6), and porcelain chipping (n = 5). Men and probable bruxism were identified in the Cox regression model as being associated with crown failure. The most common observed technical complications were mobility of the crown and chipping of the ceramic material, with the latter being observed even in crowns manufactured of monolithic zirconia. Cases with at least one technical complication (not considering loss of screw hole sealing) were more common among probable bruxers than in non-bruxers (p = 0.002). Cases of ceramic chipping were more common among bruxers than in non-bruxers (p = 0.014, log-rank test). Conclusions: Probable bruxism and patient’s sex (men) were factors associated with a higher risk of failure of implant-supported single crowns.
Development and Validation of a Panel of One-Step Four-Plex qPCR/RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Pathogens Associated with Canine Infectious Respiratory Disease Complex
Authors: Thieulent Côme J. , Carossino Mariano , Peak Laura , Strother Keith , Wolfson Wendy , Balasuriya Udeni B. R. 
Published: September 2023

Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory disease in the canine population and is caused by a wide array of viruses and bacterial pathogens with coinfections being common. Since its recognition in late 2019, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been reported to cause respiratory disease in dogs. Therefore, the rapid detection and differentiation of SARS-CoV-2 from other common viral and bacterial agents is critical from a public health standpoint. Here, we developed and validated a panel of four one-step multiplex qPCR/RT-qPCR assays for the detection and identification of twelve pathogens associated with CIRDC (canine adenovirus-2, canine distemper virus, canine herpesvirus-1, canine influenza A virus, canine parainfluenza virus, canine pneumovirus, canine respiratory coronavirus, SARS-CoV-2, Bordetella bronchiseptica, Streptococcus equi subsp. zooepidemicus, Mycoplasma cynos, and M. canis), as well as the identification of three main CIV subtypes (i.e., H3N2, H3N8, and H1N1). All developed assays demonstrated high specificity and analytical sensitivity. This panel was used to test clinical specimens (n = 76) from CIRDC-suspected dogs. M. canis, M. cynos, and CRCoV were the most frequently identified pathogens (30.3%, 25.0%, and 19.7% of samples, respectively). The newly emerging pathogens CPnV and SARS-CoV-2 were detected in 5.3% of samples and coinfections were identified in 30.3%. This new multiplex qPCR/RT-qPCR panel is the most comprehensive panel developed thus far for identifying CIRDC pathogens, along with SARS-CoV-2.
Genotoxic Effects on Daphnia magna Fed with Aquatic Green Algae Exposed to Silver Nanoclusters
Authors: Zhang Li , Tan Haoqiang 
Published: September 2023

Ag nanoclusters (AgNCs) have gained widespread applications in recent years due to their excellent antimicrobial efficacy and distinctive molecule-like characteristics. However, concerns about their potential effects on environmental and human health have been raised. Despite the fact that abundant research has been carried out to examine the possible ecotoxicology of AgNCs in a variety of living organisms, these studies have mostly concentrated on the toxicology of individual organisms and only a few have attempted to look into the impact of AgNCs across the aquatic food chain. This work evaluated the transcriptome level genotoxicity of AgNCs and their degraded Ag ions in two model species food chains: the aquatic green algae Scenedesmus obliquus and the invertebrate Daphnia magna. Daphnia magna’s digestive system and glycerophospholipid metabolism were hindered after feeding on Ag-containing algae as a result of down-regulation of the crucial gene PLA2G(SPLA2) that codes for secretory phospholipase A2. Our research also showed that the genotoxicity of AgNCs to Daphnia magna was mediated by a synergic interaction between the particulate form of AgNCs and their degraded Ag ions. The current work offers a fresh viewpoint on the mechanisms underlying AgNCs’ harmful effects and the possible ecological concern that metal-based nanoparticles provide to aquatic life.
Market Waste Management Towards a Healthy Market
Authors: Fahri Sukmal , Krisdyanta Krisdyanta , Kartika Mbulan Dewi 
Published: September 2023

Garbage is a serious problem that must be considered. The amount of waste is increasing along with the population. One of the most common waste problems is market waste. Increased activity in the market dramatically affects the number of market waste piles. To overcome this, serious waste management is needed. Waste management starts with waste collection, transportation, temporary waste disposal, and the role of cleaners and traders. This study aims to find out how the Waste Management System at the Kersik Tuo Market works.
This type of research is descriptive, using observation and interviews. The samples used were 85 traders and five janitors. The results of this study are that the Kersik Tuo Market is categorized as unhealthy where waste collection is 21 (24.7%), waste transportation is 21 (24%), availability of waste storage facilities is 21 (24%), and the role of traders is 37 (43.5%) not following provisions Permenkes No 17 of 2020 concerning Organizing Healthy Markets and the Role of Poor Traders in Waste Management. In conclusion, it is necessary to implement Government Regulation No. 17 of 2020 concerning Organizing Healthy Markets so that Kersik Tuo Market waste management can improve and make the Kersik Tuo market become a healthy market. To realize this, the participation of traders in market waste management.
Effect of Prazosin Hydrochloride in Reproductive performance of Albino Male Rats
Authors: Salih  Jaffat Haider 
Published: September 2023

This study aims to evaluate side effects of prazosin hydrochloride in fertility of male rats. Study groups were received orally graded dose of the prazosin hydrochloride (20,40, 80) mg/kg for 8-weeks, after end experimental, the treated group show significant decrement in testosterone and luteinizing hormone levels and decrement in sperm count. Also it causes decreased in follicle stimulating hormone level and testicular morphology, Sperm viability and motility. the body weight was decreased in the treated groups compare with control group, and decrease in weight of the tests, seminal vesicles and prostate gland compare with control group. The present study concluded that the prazosin hydrochloride possessed as anti-androgenic and anti spermatogenic affects.
Inferring shape transformations in a drawing task
Authors: Schmidt Filipp , Tiedemann Henning , Fleming Roland W. , Morgenstern Yaniv 
Published: September 2023

Abstract
Many objects and materials in our environment are subject to transformations that alter their shape. For example, branches bend in the wind, ice melts, and paper crumples. Still, we recognize objects and materials across these changes, suggesting we can distinguish an object’s original features from those caused by the transformations (“shape scission”). Yet, if we truly understand transformations, we should not only be able to identify their signatures but also actively apply the transformations to new objects (i.e., through imagination or mental simulation). Here, we investigated this ability using a drawing task. On a tablet computer, participants viewed a sample contour and its transformed version, and were asked to apply the same transformation to a test contour by drawing what the transformed test shape should look like. Thus, they had to (i) infer the transformation from the shape differences, (ii) envisage its application to the test shape, and (iii) draw the result. Our findings show that drawings were more similar to the ground truth transformed test shape than to the original test shape—demonstrating the inference and reproduction of transformations from observation. However, this was only observed for relatively simple shapes. The ability was also modulated by transformation type and magnitude but not by the similarity between sample and test shapes. Together, our findings suggest that we can distinguish between representations of original object shapes and their transformations, and can use visual imagery to mentally apply nonrigid transformations to observed objects, showing how we not only perceive but also ‘understand’ shape.
PENERAPAN KESEHATAN DAN KESELAMATAN KERJA RUMAH SAKIT (K3RS) DI RSUD KOTA KENDARI TAHUN 2023
Authors: Nurhanifa Wa Ode , Sabilu Yusuf , Asfian Pitrah 
Published: September 2023

Abstrak
Rumah sakit ialah komponen penting dari institusi sosial dan kesehatan yang bertujuan untuk menawarkan layanan komprehensif, kuratif, dan preventif kepada masyarakat. Kesehatan dan keselamatan staf rumah sakit, pasien, pendamping pasien, pengunjung, dan lingkungan rumah sakit harus dilindungi dari segala potensi bahaya di rumah sakit, menurut administrator rumah sakit. Tujuan penelitian adalah untuk mengetahui penerapan kesehatan dan keselamatan kerja rumah sakit (K3RS) di Rumah Sakit Umum Daerah Kota Kendari Tahun 2023” sehingga dapat memenuhi standar K3RS dan demi terciptanya derajat kesehatan yang optimal. Metode penelitian ini ialah penelitian kualitatif yang teknik pengumpulan datanya dengan pengamatan, wawancara juga pemanfaatan dokumen. Informan dalam penelitian yaitu ketua komite K3RS, koordinator bidang K3, koordinator bidang pencegahan dan pengendalian kebakaran dan staf administrasi. Hasil penelitian menunjukkan bahwa penerapan kebijakan K3RS pada tahap perencanaan K3RS 100%, pelaksananaan rencana K3RS 90% sesuai, pemantauan dan evaluasi kinerja K3RS 75% sesuai, dan Penerapan Peninjauan dan peningkatan kinerja K3RS 50% sesuai dengan Permenkes RI No. 66 tahun 2016. Kesimpulan penelitian ini yaitu penerapan K3RS belum optimal dari segi pelaksanaan rencana karena belum didukung oleh SDM di bidang K3RS yang memadai, tidak menjalin kerja sama dengan pihak lain dalam pengamatan dan penilaian kinerja K3RS, juga tidak adanya indikator kinerja yang akan dicapai khusus K3RS.

Kata Kunci : perencanaan K3RS, pemantauan dan evaluasi kinerja K3RS, pelaksanaan rencana K3RS, penerapan peninjauan dan peningkatan kinerja K3RS
PENGARUH PENYULUHAN MENGGUNAKAN MEDIA LEAFLET TERHADAP PENINGKATAN PENGETAHUAN DAN SIKAP DALAM PEMILIHAN MAKANAN JAJANAN PADA SISWA SDN 1 BIWINAPADA KABUPATEN BUTON SELATAN TAHUN 2022
Authors: Firdayatun Firdayatun , Asfian Pitrah , Erawan Putu Eka Meiyana 
Published: September 2023

Latar Belakang: Makanan jajanan merupakan makanan dan minuman yang dipersiapkan dan dijual oleh pedagang kaki lima di jalanan dan di tempat-tempat keramaian umum lain yang langsung dimakan atau dikonsumsi tanpa pengolahan atau persiapan lebih lanjut. Pada tahun 2018 terdapat sekitar 600 juta orang atau hampir 1 dari 10 orang di dunia jatuh sakit setelah mengonsumsi makanan yang terkontaminasi dan 420.000 orang dilaporkan meninggal setiap tahun akibat mengonsumsi makanan yang tidak aman.
Tujuan: Tujuan untuk mengetahui pengaruh penyuluhan menggunakan media leaflet terhadap peningkatan pengetahuan dan sikap dalam pemilihan makanan jajanan pada siswa SDN 1 Biwinapada Kabupaten Buton Selatan Tahun 2022.
Metode: Jenis penelitian yang digunakan adalah penelitian kuantitatif dengan desain penelitian pre experimental design bentuk one group pretest-posttest design. Teknik pengambilan sampel dalam penelitian ini adalah menggunakan total sampling, dengan jumlah sampel sebanyak 52 responden sesuai dengan jumlah populasi. Hasil: Hasil penelitian ini menunjukkan bahwa terdapat pengaruh penyuluhan menggunakan media leaflet terhadap peningkatan pengetahuan (p-value = 0,000) dan sikap (p-value = 0,000) dalam pemilihan makanan jajanan pada siswa SDN 1 Biwinapada Kabupaten Buton Selatan Tahun 2022. Kesimpulan: Oleh karena itu, ada pengaruh penyuluhan menggunakan media leaflet terhadap peningkatan pengetahuan dan sikap dalam pemilihan makanan jajanan pada siswa SDN 1 Biwinapada Kabupaten Buton Selatan tahun 2022.
EFEKTIVITAS PENGGUNAAN MEDIA LEAFLET TERHADAP PENINGKATAN PENGETAHUAN, SIKAP DAN TINDAKAN IBU HAMIL DALAM MENCEGAH STUNTING DI WILAYAH KERJA PUSKESMAS ABELI TAHUN 2022
Authors: Mardan Nurulfitriana , Zainuddin Asnia , Hikmawati Zainab 
Published: September 2023

Latar Belakang: Stunting merupakan keadaan pertumbuhan anak yang melambat, tinggi badannya tidak sesuai dengan umurnya dampak dari gizi kronis, yakni malnutrisi jangka panjang. Memberikan pendidikan kesehatan kepada ibu hamil untuk meningkatkan pengetahuan, sikap dan tindakan merupakan salah satu cara pencegahan stunting dengan menggunakan media promosi kesehatan seperti leaflet.
Tujuan: Penelitian ini bertujuan untuk mengetahui efektivitas penggunaan media leaflet terhadap peningkatan pengetahuan, sikap serta tindakan ibu hamil dalam mencegah stunting di wilayah kerja Puskesmas Abeli tahun 2022.
Metode: Metode penelitian yang digunakan yaitu Pre-eksperimental dan pre-test and post-test one group design. Sampel penelitian sebanyak 35 orang melalui teknik pengambilan total sampling. Uji statistik yang digunakan adalah Wilcoxon test.
Hasil: Hasil penelitian ini menunjukkan bahwa media leaflet efektif untuk meningkatkan pengetahuan, sikap serta tindakan ibu hamil dalam mencegah stunting yang diketahui melalui skor p-value pengetahuan yaitu 0,000, skor p-value sikap 0,000 serta skor p-value tindakan 0,000.
Kesimpulan: Media leaflet efektif dalam meningkatkan pengetahuan, sikap, dan tindakan ibu hamil dalam mencegah stunting sebab skor p-value < 0,05.