bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dealing with ambivalence in the practice of advanced genetic healthcare: towards an ethical choreography
Authors: Kuiper Janneke , Borry Pascal , Vears Danya , Esch Hilde Van , Cornel Martina , Hoyweghen Ine Van 
Published: March 2023

Abstract
The implementation of next-generation sequencing (NGS) in diagnostic practice has stimulated ongoing debates on how to construct and perform ‘good’ genomic care. Our multi-sited qualitative fieldwork at two large European centers for human genetics revealed tangible ambivalence in beliefs, norms, and actions in the enactment of NGS practices across sites stemming from differing expectations, interests, demands and tensions. First, ambivalence was present around the boundaries of clinical diagnostic genetic care. The overlap between research and clinical work and diagnostics and screening led to ambivalence around ‘best’ practices and norms concerning whom to offer NGS testing and how far to take testing. Secondly, the clinical value of NGS results, especially VUS and unsolicited findings, were ambivalently valued, resulting in an inconsistent approach towards these types of findings. Thirdly, ambivalence was recognized in applying guidelines in the reality of clinical practice. In practice, such norms were often not a question of either /or but were found to be more ambivalent and context depended. The ambivalence we encountered was often not made explicit or acknowledged, failing to benefit from its possibility to encourage reflexivity and change. We propose to facilitate a more clearly outspoken ethical choreography (Thompson 2014.), where ethics and science are developed iteratively whilst welcoming different perspectives and different disciplines. Pulling experiences and practices of ambivalence into the light can help to understand the points of tensions in the values and internal logic of care practices within the clinics and facilitate a more informed, transparent, and conscious direction for genetic care.
A Multiple Regression Analysis on the Relationship Among Students’ Challenging Behaviors, Teacher Training, and the Use of Aversive Behavioral Management Techniques
Authors: Mastroianni Gina Louise , Shriner Michael 
Published: March 2023

Abstract

This study used a multiple regression analysis to determine if there was a significance between the existence and intensity of student maladaptive behaviors, number of hours of teacher trainings in proactive and preventative behavior management techniques, and the number of instances a student is exposed to aversive techniques. The general problem addressed by this study was the use of aversive behavioral management techniques when students displayed challenging behaviors in the special education classroom due to teachers’ reported feelings of being underprepared in classroom management. Aversive behavioral management techniques include restraints and seclusion practices and are to be used when a student is in imminent danger of hurting themselves or people around them. This study was inspired by the work of B. F. Skinner who theorized eliminating condition response through positive reinforcement. The purpose of this study was to determine if positive and proactive behavior management techniques can reduce maladaptive behaviors. An electronic questionnaire was sent to all special education teachers in the lower section of New York City who taught in District 75, the special education district. Teachers were asked to identify if a student with a current behavior intervention plan or behavioral management plan exhibited 38 different behaviors and the intensity of those behaviors, identify the number of proactive and preventative behavior management trainings they attended in the last two years and how often the indicated student was exposed to aversive behavioral management techniques. The results showed the number of hours of teacher training on proactive and preventative behavior interventions, and the intensity of a child’s challenging behaviors significantly predicted the number of instances aversive behavioral management techniques are used in the special education classroom. If positive and proactive teacher trainings helped to reduce the existence and intensity of maladaptive behaviors, it is believed the instances of aversive behavior management techniques would also reduce. The findings of this study will help lead a discussion on the need for teacher trainings on proactive and preventative behavior management techniques.
Application experience of left hemihepatectomy with left glissonian pedicle transverse method combined cholangiolithotomy via anterior bile duct wall to treat left Hepatolithiasis
Authors: Li Junjie , Liu Lingpeng , Huang Song , Huang Mingwen , Huang Yong , Wang Zichao , Liu Hongliang 
Published: March 2023

Abstract
Objective. To investigate the clinical significance of left hemihepatectomy with left glissonian pedicle transverse method combined cholangiolithotomy via anterior bile duct wall to treat left hepatolithiasis.
Methods. A retrospective analysis was performed on the clinical data of 12 patients who underwent laparoscopic anatomic left hemihepatectomy with left glissonian pedicle transverse method combined cholangiolithotomy via anterior bile duct wall to treat left hepatolithiasis from October 2021 to October 2022. Including general information (age, sex, BMI, preoperative diagnosis), operation-related information (intraoperative blood loss, hepatic function index, operation time, postoperative complications, drainage tubes removal time, postoperative hospital stay) and postoperative pathological diagnosis.
Results. All patients, with an average age of (50±8.73) years and an average BMI of (23.61±2.47) kg/m^2, 1 male and 3 females, were successfully performed laparoscopic 0a0n6..atomic left hemihepatectomy by improved method. The median intraoperative blood loss was 150 (50, 200) mL, median operation time was 212.5 (175, 240) minutes. Hypoproteinemia occurred in 1 patient. The drainage tubes were removed on 6 (5, 9) days. The patients were discharged 8 (6, 10) days after surgery. All patients recovered well after operation.
Conclusion. Left hemihepatectomy with left glissonian transverse method combined cholangiolithotomy via anterior bile duct wall provides a new entry point to treat left hepatic stones, which simplifies the operation process and is safer and more effective. This method especially suitable for the cases with multiple biliary tract operations and difficult dissection of hilum. It can greatly improve the surgical success rate of those who are new to start this kind of operation and lack of skillful suture technique.
Analyze of factors and prognosis of eyes lost to follow-up in retinal vein occlusive disease patients receiving anti-vascular endothelial growth factor therapy
Authors: Huang Xiaoying , Zheng Minming , Lu Jiatao , Wang Xi , Zheng Zheng 
Published: March 2023

Abstract
Background:Patients with macular edema (ME) secondary to retinal vein occlusion (RVO) who received at least one intravitreal injection of anti-vascular endothelial growth factor therapy (VEGF) and lost to follow-up (LTFU) for more than six months were analyzed to investigate the factors contributing to the LTFU and the prognosis.
Method: This was a retrospective, single-center study to analyze the causes and prognosis of LTFU over six months in RVO-ME patients treated with intravitreal anti-VEGF injections at our institution from January 2019 to August 2022 and to collect patients' baseline characteristics along with the number of injections before LTFU, primary disease, best corrected visual acuity (BCVA) before LTFU and after return visit, central macular thickness (CMT), months before LTFU and after LTFU, reasons for LTFU, and complications, to analyze the factors affecting visual outcome at a return visit.
Results: This study included 125 patients with LTFU; 103 remained LTFU after six months, and 22 returned after LTFU. The common reason for LTFU was "no improvement in vision" (34.4%), followed by "transport inconvenience" (22.4%), 16 patients (12.8%) were unwilling to visit the clinic, 15 patients (12.0%) had already elected to seek treatment elsewhere, 12 patients (9.6%) were not seen in time due to the 2019-nCov epidemic, and 11 patients (8.8%) cannot do it due to financial reasons. The number of injections before LTFU was a risk factor for LTFU (P < 0.05). LogMAR at the initial visit (P< 0.001), CMT at the initial visit (P< 0.05), CMT before the LTFU (P< 0.001), and CMT after the return visit (P< 0.05) were influential factors for logMAR at the return visit.
Conclusion:Most RVO-ME patients were LTFU after anti-VEGF therapy. Long-term LTFU is greatly detrimental to the visual quality of patients; thus, the management of RVO-ME patients in follow-up should be considered.
Long-term ex-vivo normothermic perfusion of human split livers: a unique model to study new therapeutics and increase the number of available organs
Authors: Lau Ngee-Soon , Ly Mark , Dennis Claude , Jacques Andrew , Cabanes-Creus Marti , Toomath Shamus , Huang Joanna , Mestrovic Nicole , Yousif Paul , Chanda Sumon , Wang Chuanmin , Lisowski Leszek , Liu Ken , Kench James , McCaughan Geoffrey , Crawford Michael , Pulitano Carlo 
Published: March 2023

AbstractCurrent perfusion technology only allows livers to be preserved ex-vivo for short periods. Long-term normothermic perfusion of livers is an emerging field with tremendous potential for the assessment, recovery, and modification of organs. In this study, we aimed develop a long-term model of ex-vivo perfusion including a surgical split and simultaneous perfusion of both partial grafts. Our long-term perfusion system included long-term oxygenators, a gas-mixer and a dialysis filter. Human livers declined for transplantation were perfused using a red-cell based perfusate under normothermic conditions (36°C) and then split and simultaneously perfused on separate machines. Ten human livers were split resulting in 20 partial grafts. The median ex-vivo survival was 165 hours (7 days). Long-term graft survival was demonstrated by lactate clearance, bile production, Factor-V production, and storage of adenosine triphosphate. The grafts that survived > 7 days demonstrated significantly higher bile production, Factor-V production, and hepatic arterial flow and significantly lower microvesicular steatosis. We report reliable long-term ex-vivo perfusion of human livers and demonstrate the ability to split and perfuse these organs using a reproducible protocol. This provides the opportunity for improved assessment of organs and could act as a model for the testing of therapeutics with a matched control.
Effect of Traditional Chinese Medicine Combined Group Psychotherapy on Psychological Distress Management and Gut Micro-biome Regulation for Colorectal Cancer Survivors: A Single-arm Phase I Clinical Trial
Authors: Sun Lingyun , Pang Ying , Wang Zixu , Liu Jiaxi , Peng Rongyan , Yan Yunzi , Yang Yufei , Tang Lili 
Published: March 2023

Abstract
Objective: To evaluate the efficacy and feasibility of utilizing Traditional Chinese Medicine(TCM) combined group psychotherapy intervention on psychological distress management and gut micro-biome regulation for colorectal(CRC) survivors.
Methods: A single-arm phase I clinical trial was conducted between December 2020 and December 2021 in Xiyuan and Beijing Cancer Hospital in China. Inclusion criteria was stage I-III CRC survivors after radical surgery with age between 18-75. The intervention was a six-week online TCM combined group psychotherapy intervention including 90 minutes’ communication, TCM lifestyle coaching, self-acupressure guidance and mindfulness practice led by TCM oncologist and psychiatrist each week. Outcomes were measured by Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS), Fear of Cancer Recurrence Inventor (FCRI), and Quality of Life Questionnaire(QLQ-C30). Fetal samples before and after intervention were collected for 16Sr RNA analysis.
Results: We recruited 40 CRC survivors and 38 of them finally completed all interventions with average age of 58±13 years’ old. Paired t-test showed that SAS at week 2(35.4±5.8), week 4(37.9±10.5) and week 6(31.3±6.4) during the intervention was significantly lower than baseline (42.1±8.3, p<0.05 respectively). SDS score also declined substantially from baseline (38.8±10.7) to week 2(28.3±8.8, p<0.001) and week 6(25.4±7.7, p<0.001). FCRI decreased from 19.4±7.2 at baseline to 17.5±7.1 at week 4(p=0.038) and 16.3±5.8 at week 6(p=0.008). Although changes of QLQ-C30 were not statistically prominent, insomnia and fatigue significantly alleviated. The abundances of gut microbiota Intestinibacter, Terrisporobacter, Coprobacter and Gordonibacter were all significantly elevated after intervention.
Conclusions: TCM combined group psychotherapy intervention is feasible and effective to reduce CRC survivors’ psychological distress and modulate certain gut bacteria which might be associated with brain-gut axis effect. It is necessary to carry out with phase II randomized controlled clinical trial.
2D and 3D territories and territory overlap of Willow Warblers Phylloscopus trochilus in Arctic forests
Authors: Matantseva Maria V. , Simonov Sergey A. 
Published: March 2023

Abstract
Space use by animals is usually considered in two horizontal dimensions, less often with a separate analysis of the vertical stratification. Only recently, studies on three-dimensional (3D) space use appeared with modelling of individual movements simultaneously in three dimensions. In ornithology, such works were done when studying home ranges of large birds tagged with radio transmitters and GPS locators or during visual observations of colour-banded passerine wintering birds and residents in tropics. To our knowledge, such methods have not been used for studying 3D territories advertised by singing in passerines during the breeding period in the Palearctic Region. We studied the 3D territoriality of Willow Warblers Phylloscopus trochilus during the breeding period in the Kola Peninsula, Arctic Region, NW Russia. Using the method of Cooper et al. (2014), we located points with 3D coordinates at which individually marked males sang and analysed 2D and 3D territories and their overlap in R. We found that 2D methods, compared to 3D methods, overestimated individual spatial overlap by 8.5% and total spatial overlap by 16.7%. Thus, Willow Warblers in Arctic forests can partition their territorial space in 3 dimensions. With the overlap indices adopted for use with 3D data, we also proved that they could avoid areas of overlap. Furthermore, it was demonstrated for the first time that the overestimation of the territory overlaps by 2D versus 3D methods grew higher as the number of neighbour territories increased. In dense populations, 3D territories are arranged like layers of roof tiles, avoiding substantial overlaps.
Assessing receptive verb knowledge in late talkers and autistic children: Advances and cautionary tales
Authors: Horvath Sabrina , Arunachalam Sudha 
Published: March 2023

Abstract
Purpose
Using eye-tracking, we assessed the receptive verb vocabularies of late talkers and typically developing children (Experiment 1) and autistic preschoolers (Experiment 2). We evaluated how many verbs participants knew and how quickly they processed the linguistic prompt.
Method
Participants previewed two dynamic scenes side-by-side (e.g., “stretching” and “clapping”) and were then prompted to find the target verb. Children’s eye gaze behaviors were operationalized using established approaches in the field with modifications in consideration for the type of stimuli (dynamic scenes versus static images) and the populations included. Accuracy was calculated as a proportion of time spent looking to the target, and linguistic processing was operationalized as latency of children’s first look to the target.
Results
In Experiment 1, there were no group differences in the proportion of verbs known, but late talkers required longer to demonstrate their knowledge than typically developing children. Latency was predicted by age but not language abilities. In Experiment 2, autistic children’s accuracy and latency were both predicted by receptive language abilities.
Conclusion
Eye gaze can be used to assess receptive verb vocabulary in a variety of populations, but in operationalizing gaze behavior, we must account for between- and within-group differences. Bootstrapped cluster-permutation analysis is one way to create individualized measures of children’s gaze behavior, but more research is warranted using an individual differences approach with this type of analysis. Finally, latency may not be a valid measure for dynamic scene stimuli for children under three years old.
V-I penoscrotal reconfiguration: a simple new technique in the treatment of congenital webbed penis.
Authors: Bagnara Vincenzo , Berrettini Alfredo , Castagnetti Marco , Messina Mario , Angotti Rossella , Paraboschi Irene , Donà Anna Diletta 
Published: March 2023

Abstract
Introduction: Congenital webbed penis (CWP) describes a condition in which the ventral skin of the penis is fused with the median raphe of the scrotum, thereby obscuring the penoscrotal angle.
Materials and methods: We retrospectively reviewed the clinical notes of 21 patients undergoing treatment of CWP with “V-I penoscrotal reconfiguration technique”. CWP was assessed according to the classification of El-Koutby which includes Grade 1: the webbed skin reaches the proximal 1/3 of the penile shaft; Grade 2: the webbed skin extends to the mid 1/3 of the penis; Grade 3: the web extends to the distal 1/3 of the shaft. If phimosis was associated, this was graded according to Meuli’s classification into 4 degrees. The degree of parental satisfaction was quantified in 5 degrees according to the Likert Scale: “very unsatisfied”: score 0; “unsatisfied”: score 1; “neither satisfied nor unsatisfied”: score 2; “satisfied”: score 3; “very satisfied”: score 4.
Results: CWP was grade 3 in 11 patients (52%), grade 2 in 5 patients (24%), and grade 1 in 5 patients (24%). Furthermore, it was present as isolated malformation (5 cases, 24%), associated with phimosis (11 cases, 52%), associated with hypospadias (3 cases, 14%), associated with hypospadias and phimosis (2 cases, 10%). Post-operative follow-up included clinical assessments at 2 weeks and then at 1, 6, and 12 months. No postoperative complications were noted during the follow-up. Even the one-year follow-up did not show any blemishes or scar retractions with penile curvature. In no case was necessary a reoperation. The aesthetic result was excellent in all cases. Regarding the parental satisfaction score in 17 cases parents reported a score of 4 and the remaining 4 a score of 3, especially for the aspect of the circumcised penis.
Conclusions: The webbed penis is an infrequent congenital malformation, which causes psychological distress, especially in parents. Additionally, possible functional problems may arise during sexual activity in adulthood. Its correction in pediatric age is therefore desirable, to prevent possible psychological and sexual. In our opinion, the described “V-I reconfiguration technique” is simple to perform, and easy to reproduce with excellent aesthetic and functional results.
Image fusion guidance for left subclavian artery in situ fenestration during thoracic endovascular repair
Authors: Zhao Liang , Liu Jidong , Cai Xiaoshu , Yang Wengang , Wang Ji 
Published: March 2023

Abstract
Objectives
To evaluate the feasibility and clinical benefit of utilizing image fusion for thoracic endovascular repair (TEVAR) with in situ fenestration (ISF-TEVAR).
Materials and methods
Between January 2020 and December 2020, we prospectively collected 18 consecutive cases with complex thoracic aortic lesions who underwent image fusion guided ISF-TEVAR. As a control group, 18 patients were collected from historical medical records from June 2019 to December 2019. The fusion group involved the use of 3D fusion of CTA and fluoroscopic images for real-time 3D guidance, and the control group involved the use of only regular fluoroscopic images for guidance. The total contrast medium volume, hand-injected contrast medium volume, overall operative time, radiation dose and fluoroscopy time were compared between the two groups. Accuracy was measured based on preoperative CTA and intraoperative digital subtraction angiography.
Results
3D fusion imaging guidance was successfully implemented in all patients in the fusion group. Hand-injected contrast medium volume and overall operative time were significantly lower in the fusion group than in the control group (p =.028 and p =.011). Compared with the control group, the fusion group showed a significant reduction in time and radiation dose-area product (DAP) for fluoroscopy (p=.004 and p =.010). No significant differences in total radiation dose (DAP) or total contrast medium volume were observed (p =.079 and p=.443). Full accuracy was achieved in 8 cases (44%), with a mean deviation of 2.61 mm ± 3.1 (range 0.0-8.4 mm).
Conclusions
3D image fusion for ISF-TEVAR was associated with a significant reduction in hand-injected contrast medium, time and radiation exposure for fluoroscopy and overall operative time. The image fusion guidance showed potential clinical benefits towards improved treatment safety and accuracy for complex thoracic endovascular interventions.
Isolation and functional characterization of three TERMINAL FLOWER 1-like genes from Platanus acerifolia
Authors: Zhang Sisi , Zhou Qin , Yang Xingyu , Wang Jianqiang , Jiang Jie , Sun Miaomiao , Liu Yanjun , Nie Chaoren , Bao Manzhu , Liu Guofeng 
Published: March 2023

Abstract

Three TERMINAL FLOWER 1 (TFL1)-like genes were isolated and characterized from London plane tree (Platanus acerifolia). All genes have conserved genomic organization and characteristic of the phosphatidylethanolamine-binding protein (PEBP) family. Sequence alignment and phylogenetic analysis indicated that two genes belong to the TFL1 clade, designated as PlacTFL1a and PlacTFL1b, while another one was grouped in the BFT clade, named as PlacBFT. qRT-PCR analysis showed that all three genes primarily expressed in vegetative phase, but the expression of PlacTFL1a was much higher and wider than that of PlacTFL1b, with the latter only detected at relatively low expression levels in apical and lateral buds in April. PlacBFT was mainly expressed in young stems of adult trees followed by juvenile tissues. Ectopic expression of any TFL1-like gene in Arabidopsis showed phenotypes of delayed or repressed flowering. Furthermore, overexpression of PlacTFL1a gene in petunia also resulted in extremely delayed flowering. In non-flowering 35:PlacTFL1a transgenic petunia plants, the FT-like gene (PhFT) gene was significantly up-regulated and AP1 homologues PFG, FBP26 and FBP29 were significantly down-regulated. Yeast two-hybrid analysis indicated that only weak interactions were detected between PlacTFL1a and PlacFDL, and PlacTFL1a showed no interaction with PhFDL1/2. These results indicated that the TFL1-like genes of Platanus have conserved roles in repressing flowering, but probably via a distinct regulatory mechanism.