bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low light stress promotes new tiller regeneration by changing source–sink relationship and activating expression of expansin genes in wheat
Authors: Yang Hong , Li Yongpeng , Qiao Yunzhou , Sun Hongyong , Liu Wenwen , Qiao Wenjun , Li Weiqiang , Liu Mengyu , Dong Baodi 
Published: January 2023

AbstractLow light stress seriously decreased wheat grain number through the formation of aborted spike during the reproductive period and induced new tiller regeneration to offset the loss of grain number. However, the mechanism by which plants coordinate spike aborted growth and the regeneration of new tillers remains unknown. To better understand this coordinated process, morphological, physiological and transcriptomic analyses were performed under low light stress at the young microspore stage. Our findings indicated that leaves exhausted most stored carbohydrates in 1 day of darkness. However, spike and uppermost internode (UI) were converted from sink to source, due to increased abscisic acid (ABA) content and decreased cytokinin content. During this process, genes encoding amylases, Sugars Will Eventually be Exported Transporters (SWEET) and sucrose transporters or sucrose carriers (SUT/SUC) were upregulated in spike and UI, which degraded starch into soluble sugars and loaded them into the phloem. Subsequently, soluble sugars were transported to tiller node (TN) where cytokinin and auxin content increased and ABA content decreased, followed by unloading into TN cells by upregulated cell wall invertase (CWINV) genes and highly expressed H+/hexose symporter genes. Finally, expansin genes integrated the sugar pathway and hormone pathway, and regulate the formation of new tillers directly.
Adipokines from adipose tissue and common variable immunodeficiency: Is there any association?
Authors: Hassun Luana Amorim , Ruggeri Maria Luiza Ricarte , de Souza Stefany Alvino , Rossato Alice Mory , Chmieleski Gabriela Souza , de Carvalho Larissa Scarpini , Riccetto Adriana Gut Lopes , Degasperi Giovanna Rosa 
Published: January 2023

AbstractAdiponectin and leptin are adipokines, secreted by white adipose tissue (WAT), which play an important role in energy homeostasis. Some evidence has shown that adipokine‐producing adipose cells present in the bone marrow (BM) appear to exert an influence on hematopoiesis and B cell development. Common variable immunodeficiency (CVID) is one of the most common inborn errors of immunity in humans. In CVID, numerical and/or functional defects of B cells and their precursors result in hypogammaglobulinemia, usually Immunoglobulin (Ig) A and IgG. Manifestations of CVID include immunodeficiency, autoimmunity, inflammation and lymphoproliferation, resulting in a wide range of phenotypes. How adipokines interact and influence the pathophysiology of CVID is still unclear. In this review, we seek to summarize the aspects known so far concerning the interface between adipokines, B cells and CVID. More research is needed to fully understand these interactions; this knowledge is a potential avenue for the discovery of useful biomarkers and may provide new therapeutic targets for the treatment of patients with CVID and related diseases.
Efficient photocatalytic degradation of bisphenol A by green synthesized CuO decorated nickel hexacyanoferrate nanocomposite
Authors: Meenu  , Rani Manviri , Shanker Uma 
Published: January 2023

AbstractBisphenol A (BPA), a chemical additive in polycarbonate plastics and epoxy resins, is suspected to be an endocrine‐disrupter. Extensive use and irregular treating methods have led to frequent detection of BPA in wastewater, raising demand for their removal by efficient nanomaterials‐based technique. Nanocomposite CuO@NiHCF was synthesized via green method using Citrus aurantium peel extract. Crystalline structure (particle size <50 nm) of CuO@NiHCF was analysed by microscopic and spectroscopic analysis. This nanocomposite showed 97% degradation of BPA (50 mg L−1) at neutral pH in sunlight. Moreover, improved particle stability (zeta potential: −56.2 mV; 2.0 eV) and high surface area, pore volume (81 m2g−1, 13.9 nm) resulted from synergism of NiHCF (−26.3 mV; 2.4 eV), and CuO (−11.5 mV; 1.9 eV) led to efficient photodegradation of BPA. Degradation of BPA was found photo‐adsorptive. Moreover, degradation was carried out by OH radicals based with ring‐opening mechanisms by GC–MS. High efficiency and sustainability of CuO@NiHCF were revealed by its multiple reusability (n = 10), leading to a promising and sustainable photocatalyst.
Measurement of Acoustoelastic Coefficients in Concrete Using Thermal Modulation of Ultrasonic Waves
Authors: Zhong Bibo , Zhu Jinying 
Published: January 2023

Abstract
This study develops a new method to determine the acoustoelastic coefficient in concrete using thermally-induced ultrasonic bulk wave velocity changes. This paper presents the equations of wave velocity changes caused by homogeneous temperature variation and uniaxial strain in an isotropic medium. The acoustoelastic coefficient of a concrete sample was calculated using the third-order elastic constants (l, m and n) which were determined in the thermal modulation test. The acoustoelastic coefficient of the same sample was then experimentally verified in a four-point bending test. The results showed that relative wave velocity change predicted by the third-order elastic constants from the thermal modulation test agreed well with the experimental value from the bending test.
Compressive Sensing and Deep Learning Enhanced Imaging Algorithm for Sparse Guided Wave Array
Authors: Wang Xiaocen , Lin Min , Li Jian , Wang Dingpeng , Liu Yang 
Published: January 2023

Abstract
Aiming at the problem of image quality reduction caused by sparse array in guided wave detection, an enhanced algorithm based on improved compressive sensing and deep learning is proposed in this paper so as to realize high-quality imaging with a small number of sensors. The enhancement algorithm consists of two parts: the sparse guided wavefield is up-sampled by the improved compressed sensing, and then the up-sampled guided wavefield is input into U-net for further recovery. After compressive sensing and deep learning enhancement, the recovered wavefield is close to the dense wavefield. Simulation is carried out and the results verify the feasibility of the method. In training and validation, the losses evaluated by mean square error (MSE) are 1.62 × 10-4 and 2.18 × 10-5 for 32 sensors and 1.65 × 10-4 and 3.44 × 10-5 for 16 sensors. Imaging performance is also verified by Pearson’s coefficient. The Pearson’s coefficient is improved from 0.9218 to 0.9517 with 32 sensors, and improved from 0.8896 to 0.9487 with 16 sensors.
Using a generic quality of life measure to determine adherence thresholds: a cross-sectional study on older adults with neurological disorders in Germany
Authors: Mühlhammer Hannah M , Schönenberg Aline , Lehmann Thomas , Prell Tino 
Published: January 2023

ObjectivesMeasuring the degree of adherence to medication is essential in healthcare However, the cut-offs provided for adherence scales are often arbitrary and disease-specific, and need to be validated against a clinical outcome. Here, we used health-related quality of life (QoL) to determine cut-offs for a self-report adherence questionnaire in patients with neurological diagnoses.DesignCross-sectional study.Participants910 patients (age 70±8.6 years) with neurological disorders were recruited from the wards of neurology at a local university hospital. All patients received a comprehensive geriatric assessment, including assessments of adherence (Stendal Adherence to Medication Score, SAMS) and QoL (Short Form Survey SF-36).Outcome measuresThe main aim of the study was to define a cut-off for non-adherence at which QoL is significantly impaired. Thus, we used Spearman’s rank correlation, multivariate and univariate analyses of variance to test the impact of different adherence levels on QoL. Receiver operating characteristics and area under curve measures were then used to determine cut-off scores for adherence based on significant differences in QoL.ResultsCorrelations between SAMS and SF-36 domains were weak (ranging between r=−0.205 for emotional well-being and r=−0.094 for pain) and the effect of non-adherence on QoL disappeared in the multivariate analysis of variance (p=0.522) after adjusting for demographical and clinical factors. SAMS cut-offs in terms of SF-36 domains varied greatly, so that an overall SAMS cut-off for this cohort could not be defined.ConclusionsQoL as measured by the SF-36 is not suitable as a single outcome parameter to study the impact of non-adherence on QoL in a mixed neurological cohort. Since both QoL and adherence are heterogeneous, multifaceted constructs, it is unlikely to find an overarching cut-off applicable for all patients. Thus, it may be necessary to use disease or cohort-specific external outcome parameters to measure the indirect effect of interventions to enhance adherence.Trial registration numberDRKS00016774.
Fear of making a mistake: a prominent cause of stress for COVID-19 ICU staff—a mixed-methods study
Authors: Escher Cecilia , Nagy Elisabeth , Creutzfeldt Johan , Dahl Oili , Ruiz Mini , Ericson Mats , Osika Walter , Meurling Lisbet 
Published: January 2023

IntroductionThe COVID-19 pandemic has had a profound effect on many domains of healthcare. Even in high-income countries such as Sweden, the number of patients has vastly outnumbered the resources in affected areas, in particular during the first wave. Staff caring for patients with COVID-19 in intensive care units (ICUs) faced a very challenging situation that continued for months. This study aimed to describe burnout, safety climate and causes of stress among staff working in COVID-19 ICUs.MethodA survey was distributed to all staff working in ICUs treating patients with COVID-19 in five Swedish hospitals during 2020 and 2021. The numbers of respondents were 104 and 603, respectively. Prepandemic data including 172 respondents from 2018 served as baseline.ResultsStaff exhaustion increased during the pandemic, but disengagement decreased compared with prepandemic levels (p<0.001). Background factors such as profession and work experience had no significant impact, but women scored higher in exhaustion. Total workload and working during both the first and second waves correlated positively to exhaustion, as did being regular ICU staff compared with temporary staff. Teamwork and safety climate remained unchanged compared with prepandemic levels.Respondents reported ‘making a mistake’ as the most stressful of the predefined stressors. Qualitative analysis of open-ended questions identified ‘lack of knowledge and large responsibility’, ‘workload and work environment’, ‘uncertainty’, ‘ethical stress’ and ‘organization and teamwork’ as major causes of stress.ConclusionDespite large workloads, disengagement at work was low in our sample, even compared with prepandemic levels. High levels of exhaustion were reported by the ICU staff who carried the largest workload. Multiple significant causes of stress were identified, with fear of making a mistake the most significant stressor.
Win or lose, nighttime transcystic laparoscopic common bile duct exploration is a win
Authors: Stettler Gregory R , Ganapathy Aravindh S , Bosley Maggie E , Spencer Audrey L , Neff Lucas P , Nunn Andrew M , Miller Preston R 
Published: January 2023

ObjectivesAlthough controversial, recent data suggest nighttime versus daytime laparoscopic cholecystectomy (LC) have comparable outcomes. Laparoscopic common bile duct exploration (LCBDE) for choledocholithiasis decreases length of stay (LOS) as compared with LC with endoscopic retrograde cholangiopancreatography (ERCP) but increases case complexity/time. The influence of time of day on LCBDE outcomes has not been evaluated. Our aim was to examine outcomes and LOS for nighttime (PM) compared with daytime LC+LCBDE (DAY).MethodsConsecutive patients who underwent LCBDE were reviewed. Demographics, operative duration, success of LCBDE, time to postoperative ERCP (if required), LOS, and complications were compared. PM procedures were defined as beginning 19:00–07:00 hours.ResultsBetween 2018 and 2022, sixty patients underwent LCBDE (PM 42%). Groups had equivalent age/sex and preoperative liver function tests (LFTs). LCBDE success was 69% PM versus 71% DAY (p=0.78). Operative duration did not differ (2.8 IQR: 2.2–3.3 hours vs. 2.8 IQR: 2.3–3.2 hours, p=0.9). LOS was compared, and PM LOS was shorter (p=0.03). Time to ERCP after a failed LCBDE at night was compared with daytime (13.8 IQR: 10.6–29.5 hours vs. 19.9 IQR: 18.7–54.4 hours, p=0.07). LOS forfailedPM LCBDE requiring ERCP was similar tosuccessfulDAY LCBDE (p=0.29). One complication (transient hyperbilirubinemia) was reported in the DAY group, none in PM.ConclusionPM LCBDE cases are equivalent in safety and success rate to DAY cases but have reduced LOS. Widespread adoption of acute care surgery-driven management of choledocholithiasis via LCBDE during cholecystectomy may decrease LOS, especially in PM cases.Level of evidenceLevel IV.
Stateful Protocol Composition in Isabelle/HOL
Authors: Hess Andreas V. , MÖdersheim Sebastian A. , Brucker Achim D. 
Published: January 2023

Communication networks like the Internet form a large distributed system where a huge number of components run in parallel, such as security protocols and distributed web applications. For what concerns security, it is obviously infeasible to verify them all at once as one monolithic entity; rather, one has to verify individual components in isolation.
While many typical components like TLS have been studied intensively, there exists much less research on analyzing and ensuring the security of the composition of security protocols. This is a problem since the composition of systems that are secure in isolation can easily be insecure. The main goal of compositionality is thus a theorem of the form: given a set of components that are already proved secure in isolation and that satisfy a number of easy-to-check conditions, then also their parallel composition is secure. Said conditions should of course also be realistic in practice, or better yet, already be satisfied for many existing components. Another benefit of compositionality is that when one would like to exchange a component with another one, all that is needed is the proof that the new component is secure in isolation and satisfies the composition conditions—without having to re-prove anything about the other components.

This article has three contributions over previous work in parallel compositionality. First, we extend the compositionality paradigm to
stateful systems
: while previous approaches work only for simple protocols that only have a local session state, our result supports participants who maintain long-term
databases
that can be
shared
among several protocols. This includes a paradigm for
declassification of shared secrets
. This result is in fact so general that it also covers many forms of
sequential composition
as a special case of stateful parallel composition. Second, our compositionality result is formalized and proved in Isabelle/HOL, providing a strong correctness guarantee of our proofs. This also means that one can prove, without gaps, the security of an entire system in Isabelle/HOL, namely the security of components in isolation and the composition conditions, and thus derive the security of the entire system as an Isabelle theorem. For the components one can also make use of our tool PSPSP that can perform automatic proofs for many stateful protocols. Third, for the compositionality conditions we have also implemented an automated check procedure in Isabelle.
PGC1α Degradation Suppresses Mitochondrial Biogenesis to Confer Radiation Resistance in Glioma
Authors: Zhao Mengjie , Li Yanhui , Lu Chenfei , Ding Fangshu , Xu Miao , Ge Xin , Li Mengdie , Wang Zhen , Yin Jianxing , Zhang Junxia , Wang Xiefeng , Ge Zehe , Xiao Hong , Xiao Yong , Liu Hongyi , Liu Wentao , Cao Yuandong , Wang Qianghu , You Yongping , Wang Xiuxing , Yang Kun , Shi Zhumei , Qian Xu 
Published: January 2023

AbstractRadiotherapy is a major component of standard-of-care treatment for gliomas, the most prevalent type of brain tumor. However, resistance to radiotherapy remains a major concern. Identification of mechanisms governing radioresistance in gliomas could reveal improved therapeutic strategies for treating patients. Here, we report that mitochondrial metabolic pathways are suppressed in radioresistant gliomas through integrated analyses of transcriptomic data from glioma specimens and cell lines. Decreased expression of peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC1α), the key regulator of mitochondrial biogenesis and metabolism, correlated with glioma recurrence and predicted poor prognosis and response to radiotherapy of patients with glioma. The subpopulation of glioma cells with low-mitochondrial-mass exhibited reduced expression of PGC1α and enhanced resistance to radiotherapy treatment. Mechanistically, PGC1α was phosphorylated at serine (S) 636 by DNA-dependent protein kinase in response to irradiation. Phosphorylation at S636 promoted the degradation of PGC1α by facilitating its binding to the E3 ligase RNF34. Restoring PGC1α activity with expression of PGC1α S636A, a phosphorylation-resistant mutant, or a small-molecule PGC1α activator ZLN005 increased radiosensitivity of resistant glioma cells by reactivating mitochondria-related reactive oxygen species production and inducing apoptotic effects both in vitro and in vivo. In summary, this study identified a self-protective mechanism in glioma cells in which radiotherapy-induced degradation of PGC1α and suppression of mitochondrial biogenesis play a central role. Targeted activation of PGC1α could help improve response to radiotherapy in patients with glioma.Significance:Glioma cells reduce mitochondrial biogenesis by promoting PGC1α degradation to promote resistance to radiotherapy, indicating potential therapeutic strategies to enhance radiosensitivity.
TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A. 
Published: January 2023

AbstractBreast cancer subtypes and their phenotypes parallel different stages of the mammary epithelial cell developmental hierarchy. Discovering mechanisms that control lineage identity could provide novel avenues for mitigating disease progression. Here we report that the transcriptional corepressor TLE3 is a guardian of luminal cell fate in breast cancer and operates independently of the estrogen receptor. In luminal breast cancer, TLE3 actively repressed the gene-expression signature associated with highly aggressive basal-like breast cancers (BLBC). Moreover, maintenance of the luminal lineage depended on the appropriate localization of TLE3 to its transcriptional targets, a process mediated by interactions with FOXA1. By repressing genes that drive BLBC phenotypes, including SOX9 and TGFβ2, TLE3 prevented the acquisition of a hybrid epithelial–mesenchymal state and reduced metastatic capacity and aggressive cellular behaviors. These results establish TLE3 as an essential transcriptional repressor that sustains the more differentiated and less metastatic nature of luminal breast cancers. Approaches to induce TLE3 expression could promote the acquisition of less aggressive, more treatable disease states to extend patient survival.Significance:Transcriptional corepressor TLE3 actively suppresses SOX9 and TGFβ transcriptional programs to sustain the luminal lineage identity of breast cancer cells and to inhibit metastatic progression.
Dwarf Open Reading Frame (DWORF) Gene Therapy Ameliorated Duchenne Muscular Dystrophy Cardiomyopathy in Aged mdx Mice
Authors: Morales Emily D. , Yue Yongping , Watkins Thais B. , Han Jin , Pan Xiufang , Gibson Aaron M. , Hu Bryan , Brito‐Estrada Omar , Yao Gang , Makarewich Catherine A. , Babu Gopal J. , Duan Dongsheng 
Published: January 2023

Background
Cardiomyopathy is a leading health threat in Duchenne muscular dystrophy (DMD). Cytosolic calcium upregulation is implicated in DMD cardiomyopathy. Calcium is primarily removed from the cytosol by the sarcoendoplasmic reticulum calcium ATPase (SERCA). SERCA activity is reduced in DMD. Improving SERCA function may treat DMD cardiomyopathy. Dwarf open reading frame (DWORF) is a recently discovered positive regulator for SERCA, hence, a potential therapeutic target.

Methods and Results

To study DWORF's involvement in DMD cardiomyopathy, we quantified DWORF expression in the heart of wild‐type mice and the mdx model of DMD. To test DWORF gene therapy, we engineered and characterized an adeno‐associated virus serotype 9–DWORF vector. To determine if this vector can mitigate DMD cardiomyopathy, we delivered it to 6‐week‐old mdx mice (6×10
12
vector genome particles/mouse) via the tail vein. Exercise capacity, heart histology, and cardiac function were examined at 18 months of age. We found DWORF expression was significantly reduced at the transcript and protein levels in mdx mice. Adeno‐associated virus serotype 9–DWORF vector significantly enhanced SERCA activity. Systemic adeno‐associated virus serotype 9‐DWORF therapy reduced myocardial fibrosis and improved treadmill running, electrocardiography, and heart hemodynamics.

Conclusions
Our data suggest that DWORF deficiency contributes to SERCA dysfunction in mdx mice and that DWORF gene therapy holds promise to treat DMD cardiomyopathy.
Covid-19: Exploring medico-legal perceptions amongst orthopaedic surgeons and lawyers
Authors: Seraj Shaikh S , Khadabadi Nikhil A , Jawaid Anam , Logan Peter C , Moores Thomas S , Hossain Fahad S 
Published: January 2023

Introduction British national guidelines and laws published by the British Orthopaedic Association and the new Coronovirus Act 2020 favoured treatment of trauma and orthopaedic conditions with non-operative alternatives. A survey was developed for both lawyers and trauma and orthopaedic clinicians to gauge their perceptions on guidelines related to protection of trauma and orthopaedic staff, and on prosecution with respect to future claims. Material and methods Sixteen questions were designed for surgeons and 11 questions for lawyers. The level of experience and career stages were explored in other questions. A Likert scale (0–5) was used to capture these perceptions. Results Clinicians envisaged themselves being less protected (mean = 2.6), forecasted a rise in negligence claims (mean = 3.4) and perceived little additional beneficial indemnity influence from the NHS (mean = 1.8). Lawyers felt that public perception would have more influence in negligence claim rates (mean = 2.6) and disapproved of complete immunity for clinicians (mean = 0.5). Disparities between different trauma and orthopaedic grades demonstrated sentiments of comfort with redeployment, preparedness in non-orthopaedic training and protection from litigation. Discussion The results reflected the overall anxiety over litigation reprisal shared amongst trauma and orthopaedic staff. Issues with providing sub-optimal care can worsen this overall fear. Feeling unprotected from litigation reprisal can leave clinicians with an additional sense of emotional and professional burden. Redeployment into unfamiliar environments can leave senior clinicians in limbo in contrast to their juniors. Conclusion Non-surgical options to treat orthopaedic conditions affect both patients and trauma and orthopaedic staff. Feedback from lawyers reassures trauma and orthopaedic clinicians that negligence claims should not rise due to the updated national guidelines.