bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reversed‐phase high‐performance liquid chromatography analytical method development and validation for cefepime and sulbactam injection formulation assay: A quality by design approach
Authors: Mondal Sitanshu , Asati Vivek , Verma Sant Kumar , Gupta Ghanshyam Das , Kurmi Balak Das , Patel Preeti 
Published: January 2023

AbstractA trustworthy high‐performance liquid chromatography approach for the simultaneous measurement of cefepime and sulbactam was created using quality by design. Finding reliable chromatographic settings for quality peaks and suitable component separation within a manageable run time was the key objective. Critical quality attributes for risk measurement were evaluated. To create an analytical design space and create a control strategy, the quantitative link between critical material attributes and critical quality attributes was built. Acetonitrile volume, trimethylamine volume, and flow rate were the three independent parameters considered in the mathematical models. Acetonitrile:high‐performance liquid chromatography water:triethylamine and pH 6.0, adjusted with orthophosphoric acid (50.0:50:0.15, v/v/v) as the mobile phase at 1.1 ml/min, were the ideal and anticipated conditions. We obtained appropriate baseline separation of both medications in about 10 min using these ideal conditions. The main determinant of the perturbation plot responses was found to be the quantity of acetonitrile. At all three levels, the percent recoveries for both medications ranged from 99% to 99.68%. Relative standard deviations were less than 2% for both drugs' intermediate accuracy and repeatability. A straightforward, precise, and repeatable high‐performance liquid chromatography method for regular quality control testing was created as a consequence.
Development and validation of a liquid chromatography‐tandem mass spectrometry method for the determination of temozolomide in mouse brain tissue
Authors: Kakarla Raghavi , Yacoub Kimberly , Bearden Rebecca L , Zhou Aimin , Mukherjee Sanjib , Shan Frank Y. , Guo Baochuan 
Published: January 2023

AbstractTemozolomide is a Food and Drug Administration‐approved anticancer drug that has poor drug delivery via oral or intravenous routes. A potential strategy to combat this problem is investigating alternative routes of administration, requiring quantitation of the drug in the brain tissues by liquid chromatography‐mass spectrometry. However, current methods used to extract the drug from brain tissues resulted in poor recovery and substantial matrix effects. Herein, we reported a new two‐step extraction method that involves the use of Proteinase K to lyse tumor tissues to efficiently release the drug, followed by ethanol protein precipitation. The extracts were then separated on a C18 column and analyzed in positive electrospray ionization, a multiple reaction monitoring mode of the triple quadrupole. We found this new method led to a recovery of 82% with negligible matrix effects. The method has been validated in accordance with Food and Drug Administration guidance for linearity, specificity, selectivity, accuracy, precision, carry‐over, stability, and lower limit of quantitation. In conclusion, we have developed and validated a liquid chromatography‐mass spectrometry method with a novel sample preparation method that was able to efficiently extract temozolomide from mouse brain tissue with high recovery.
Development of a thin layer chromatographic method for the determination of coenzyme Q10produced byAgrobacterium tumefaciens
Authors: Yadav Karuna , Jatain Indu , Dubey Kashyap Kumar , Nitharwal Ram Gopal , Kaur Inderjeet 
Published: January 2023

AbstractCoenzyme Q10is biosynthesized by living organisms and considered a medically necessary health constituent thereby drawing the attention of the market. Naturally‐producing microbes are commonly used for the commercial production of coenzyme Q10. The detection of extracted coenzyme Q10is usually done by high‐performance liquid chromatography and mass spectrometry methods which require sophisticated instrumentation and trained personnel. However, routine quality assessment is required rapidly at multiple steps during the large‐scale production process which necessitates the development of a rapid and relatively easy method for coenzyme Q10detection. In the present study, a thin‐layer chromatography method is developed for the rapid detection of coenzyme Q10extracted from the natural producerAgrobacterium tumefaciens. Various mobile phases were screened for the thin‐layer chromatography of coenzyme Q10and the optimum separation‐detection was observed with methanol and ethyl acetate (8:2, v/v). The same extracted coenzyme Q10was also analyzed by high‐performance liquid chromatography (the standard method of detection), showing a similar profile as with commercially available standard coenzyme Q10. Furthermore, extract from unstained thin‐layer chromatography spot showed the characteristic spectrum (ultraviolet‐visible) as with standard coenzyme Q10. Conclusively, a simple and inexpensive method for the rapid detection of coenzyme Q10was developed to aid in the commercial production of this medically important coenzyme.
Development of a thin‐layer chromatographic method for detection of lipstatin produced by Streptomyces toxytricini
Authors: Jatain Indu , Kumar Ravi , Dubey Kashyap Kumar , Yadav Karuna , Nitharwal Ram Gopal 
Published: January 2023

AbstractLipstatin or its saturated analog (orlistat), being the irreversible inhibitor of intestinal lipase, is widely used for the treatment of obesity. The bacterium Streptomyces toxytricini is the main source for the production of lipstatin/orlistat. There are continuous attempts to increase the production of lipstatin in S. toxytricini. For optimization of best conditions/strains for lipstatin production, there are requirements for a fast, simple, and reliable method to detect/estimate lipstatin. At present, highly sophisticated methods such as high‐performance liquid chromatography, mass spectrometry, and so forth are available for the detection and estimation of lipstatin/orlistat. These methods are very costly, time‐consuming, and require state of the art facility. Here we report a simple, fast, cost‐effective method based on thin‐layer chromatography for the detection of lipstatin in S. toxytricini. The optimized mobile phase was acetone: ethanol in a ratio of 3:7. Development of thin‐layer chromatography was done by ρ‐anisaldehyde staining that showed the pink color to the silica plate and greenish‐blue color to the lipstatin/orlistat drug. Conclusively, here we mention a simple and reproducible method of detection of orlistat and lipstatin samples extracted from S. toxytricini.
Non‐regenerative immune‐mediated anaemia in a dog managed following splenectomy and ovarian remnant removal
Authors: Warwick Harry , Harris Benjamin , Venier Francesca , Rosa Chantal 
Published: January 2023

AbstractA 3‐year‐old, female, neutered, crossbreed dog was diagnosed with non‐regenerative immune‐mediated anaemia. Bone marrow cytology was hypercellular, with signs of maturation arrest and rubriphagocytosis. During the initial investigations, a suspected ovarian remnant was identified. The patient was stabilised with a packed red blood cell transfusion and started on immunosuppressive medication. The anaemia remained non‐regenerative and gradually worsened over the next 2 months. Due to lack of response, splenectomy and ovarian remnant excision was performed. Splenic histopathology revealed evidence of erythrophagia. Two weeks following surgery, complete blood count revealed mild reticulocytosis, and the patient subsequently achieved clinical remission. Twenty‐one months following splenectomy, the patient continued to do well. Splenectomy may be considered as an additional immune‐modulating option in refractory cases of canine non‐regenerative immune‐mediated anaemia.
Psorergatic mange in two ring‐tailed lemurs (Lemur catta)
Authors: Sailler Anaïs , Laidebeure Sylvie , Prieto Morgane , Lemberger Karin , Lécu Alexis 
Published: January 2023

AbstractTwo 6‐year‐old ring‐tailed lemurs (Lemur catta), housed in two different zoological institutions, were evaluated for nonpruritic nodules located on the eyelids, lips and chest. The first lemur was anaesthetized for diagnostic investigations. Although pan‐herpes polymerase chain reaction was positive and bacterial culture yielded Staphylococcus warneri, histopathology of skin biopsies revealed a severe chronic dermatitis and folliculitis with intralesional arthropods, identified as Psorobia sp. on skin scrapings. The second lemur was also diagnosed a psorergatic mange. After several treatment failures, weekly topical applications of amitraz were performed and a minimum of five applications were necessary to notice the complete resolution of the lesions. Amitraz toxicity was not observed, despite the first lemur being pregnant at the time of treatment. This is the first report of psorergatic mange in a prosimian species and the first reported use of amitraz as a successful treatment for this disease.
Classification of racehorse limb radiographs using deep convolutional neural networks
Authors: Costa da Silva Raniere Gaia , Mishra Ambika Prasad , Riggs Christopher Michael , Doube Michael 
Published: January 2023

AbstractPurposeTo assess the capability of deep convolutional neural networks to classify anatomical location and projection from a series of 48 standard views of racehorse limbs.Materials and methodsRadiographs (N = 9504) of horse limbs from image sets made for veterinary inspections by 10 independent veterinary clinics were used to train, validate and test (116, 40 and 42 radiographs, respectively) six deep learning architectures available as part of the open source machine learning framework PyTorch. The deep learning architectures with the best top‐1 accuracy had the batch size further investigated.ResultsTop‐1 accuracy of six deep learning architectures ranged from 0.737 to 0.841. Top‐1 accuracy of the best deep learning architecture (ResNet‐34) ranged from 0.809 to 0.878, depending on batch size. ResNet‐34 (batch size = 8) achieved the highest top‐1 accuracy (0.878) and the majority (91.8%) of misclassification was due to laterality error. Class activation maps indicated that joint morphology, not side markers or other non‐anatomical image regions, drove the model decision.ConclusionsDeep convolutional neural networks can classify equine pre‐import radiographs into the 48 standard views including moderate discrimination of laterality, independent of side marker presence.
Significance of age and sex in botulinum neurotoxin dosing for adductor spasmodic dysphonia
Authors: Heyes Richard , Adler Charles H. , Zhang Nan , Lott David G. , Bansberg Stephen F. 
Published: January 2023

AbstractObjectivesThis study aims to analyze the impact of age and sex on botulinum neurotoxin (BoNT‐A) dosing and outcomes in adductor spasmodic dysphonia (AdSD).MethodsA database review of all spasmodic dysphonia patients treated with BoNT from 1989 to 2018 at the Mayo Clinic in Arizona was performed. Only patients who had received ≥4 injections of BoNT‐A for AdSD were included. Patients were divided into two cohorts to analyze age, with an age of first treatment cutoff of 60 years. Patients were divided into male and female cohorts to analyze sex.ResultsThe final analysis included 398 patients. The mean dose of BoNT‐A per treatment was significantly higher in the younger cohort (4.4 vs. 3.9 units, p = 0.048). The mean maximal benefit was similar (72% vs. 70%, p = 0.48); however, the mean length of benefit was significantly shorter in younger patients (3.0 vs. 3.6 months, p < 0.01). The mean BoNT‐A dose was significantly higher in the female cohort (4.2 vs. 3.6 units, p = 0.02). The mean maximal benefit was similar (69% vs. 75%, p = 0.58), as was the mean length of benefit (3.2 vs. 3.5 months, p = 0.11).ConclusionsThis study suggests that age and sex influence BoNT‐A dosing and outcomes in AdSD.
Facial nerve monitoring in parotid gland surgery: Design and feasibility assessment of a potential standardized technique
Authors: Duque Carlos S. , Londoño Andrés F. , Duque Ana M. , Zuleta Jhon J. , Marulanda Marcela , Otálvaro Lina M. , Agudelo Miguel , Dueñas Juan P. , Palacio María F. , Dionigi Gianlorenzo 
Published: January 2023

AbstractBackgroundEven though the use of nerve monitoring during parotid gland surgery is not the gold standard to prevent damage to the nerve, it surely offers some advantages over the traditional approach. Different from thyroid surgery, where a series of steps in intraoperative nerve monitoring have been described to confirm not only the integrity but—most importantly—the function of the recurrent laryngeal nerve, in parotid gland surgery, a formal guideline to follow while dissecting the facial nerve has yet to be described.MethodsA five‐year retrospective study was done reviewing the intraoperative records of patients who underwent parotid gland surgery under neural monitoring. The operative findings regarding the neuromonitoring process, particularly in regard to the amplitude of two main branches, were revised. A literature search was done to search for guidelines to follow when a facial nerve loss of signal is encountered.ResultsFifty‐five patients were operated on using the Nim 3 Nerve Monitoring System (Medtronic); 31 were female patients, and 47 patients had benign lesions. Minimum changes were observed in the amplitude records after a comparison was made between the first and the last stimulation. There were only three articles discussing the term loss of signal during parotid gland surgery.ConclusionToday, no sufficient attention has been given to the facial nerve monitoring process during parotidectomy. This study proposes a formal guideline to follow during this procedure as well as an instruction to consider when a loss of signal is observed to develop a uniform technique of facial nerve stimulation
Flow-oriented perturbation theory
Authors: Borinsky Michael , Capatti Zeno , Laenen Eric , Salas-Bernárdez Alexandre 
Published: February 2023

Abstract
We introduce a new diagrammatic approach to perturbative quantum field theory, which we call flow-oriented perturbation theory (FOPT). Within it, Feynman graphs are replaced by strongly connected directed graphs (digraphs). FOPT is a coordinate space analogue of time-ordered perturbation theory and loop-tree duality, but it has the advantage of having combinatorial and canonical Feynman rules, combined with a simplified iε dependence of the resulting integrals. Moreover, we introduce a novel digraph-based representation for the S-matrix. The associated integrals involve the Fourier transform of the flow polytope. Due to this polytope’s properties, our S-matrix representation exhibits manifest infrared singularity factorization on a per-diagram level. Our findings reveal an interesting interplay between spurious singularities and Fourier transforms of polytopes.
Zeros Under Unitary Weighted Composition Operators in the Hardy and Bergman Spaces
Authors: Montes-Rodríguez Alfonso 
Published: January 2023

AbstractLet $$\mathbb {D}$$
D
denote the unit disk of the complex plane and let $$\mathcal {A}^2(\mathbb {D})$$

A

2

(
D
)

be the Bergman space that consists of those analytic functions on $$\mathbb {D}$$
D
that are of integrable square modulus with respect to the normalized area measure. Let $$\varphi : \mathbb {D} \rightarrow \mathbb {D}$$

φ
:
D
→
D

be an automorphism of the disk and consider $$C_\varphi f=f \circ \varphi $$

C
φ

f
=
f
∘
φ

the operator defined from $$\mathcal {A}^2(\mathbb {D})$$

A

2

(
D
)

onto itself. Consider the unitary operator $$U_\varphi f = \varphi ^\prime f \circ \varphi $$

U
φ

f
=

φ
′

f
∘
φ

. Then if $$f \in \mathcal {A}^2(\mathbb {D})$$

f
∈

A

2

(
D
)

is even and $$U_\varphi f$$

U
φ

f

is odd, then f is the zero function. The same is true if $$f \in \mathcal {A}^2(\mathbb {D})$$

f
∈

A

2

(
D
)

is odd and $$U_\varphi f$$

U
φ

f

is even. Similar results can be proved for the Hardy space of the unit disk, that is, the space of analytic functions on $$\mathbb {D}$$
D
, whose Taylor coefficients are of summable square modulus. The result remains true for the Dirichlet space, that is, the space of analytic functions on $$\mathbb {D}$$
D
, whose derivatives are in $$\mathcal {A}^2(\mathbb {D})$$

A

2

(
D
)

.
Deformations of Hyperelliptic and Generalized Hyperelliptic Polarized Varieties
Authors: Bangere Purnaprajna , Gallego Francisco Javier , González Miguel 
Published: January 2023

AbstractThe purpose of this article is twofold. Firstly, we address and completely solve the following question: Let (X, L) be a smooth, hyperelliptic polarized variety and let $$\varphi : X \longrightarrow Y \subset \textbf{P}^N$$

φ
:
X
⟶
Y
⊂

P
N

be the morphism induced by |L|; when does $$\varphi $$
φ
deform to a birational map? Secondly, we introduce the notion of “generalized hyperelliptic varieties” and carry out a study of their deformations. Regarding the first topic, we settle the non trivial, open cases of (X, L) being Fano-K3 and of (X, L) having dimension $$m \ge 2$$

m
≥
2

, sectional genus g and $$L^m=2g$$

L
m

=
2
g

. This was not addressed by Fujita in his study of hyperelliptic polarized varieties and requires the introduction of new methods and techniques to handle it. In the Fano-K3 case, all deformations of (X, L) are again hyperelliptic except if Y is a hyperquadric. By contrast, in the $$L^m=2g$$

L
m

=
2
g

case, with one exception, a general deformation of $$\varphi $$
φ
is a finite birational morphism. This is especially interesting and unexpected because, in the light of earlier results, $$\varphi $$
φ
rarely deforms to a birational morphism when Y is a rational variety, as is our case. The Fano-K3 case contrasts with canonical morphisms of hyperelliptic curves and with hyperelliptic K3 surfaces of genus $$g \ge 3$$

g
≥
3

. Regarding the second topic, we completely answer the question for generalized hyperelliptic polarized Fano and Calabi–Yau varieties. For generalized hyperelliptic varieties of general type we do this in even greater generality, since our result holds for Y toric. Standard methods in deformation theory do not work in the present setting. Thus, to settle these long standing open questions, we bring in new ideas and techniques building on those introduced by the authors concerning deformations of finite morphisms and the existence and smoothings of certain multiple structures. We also prove a new general result on unobstructedness of morphisms that factor through a double cover and apply it to the case of generalized hyperelliptic varieties.
Estimates for L-functions in the Critical Strip Under GRH with Effective Applications
Authors: Simonič Aleksander 
Published: January 2023

AbstractAssuming the generalized Riemann hypothesis, we provide explicit upper bounds for moduli of $$\log {\mathcal {L}(s)}$$

log

L
(
s
)

and $$\mathcal {L}'(s)/\mathcal {L}(s)$$

L

′

(
s
)

/
L

(
s
)

in the neighbourhood of the 1-line when $$\mathcal {L}(s)$$

L
(
s
)

are the Riemann, Dirichlet and Dedekind zeta-functions. To do this, we generalize Littlewood’s well-known conditional result to functions in the Selberg class with a polynomial Euler product, for which we also establish a suitable convexity estimate. As an application, we provide conditional and effective estimates for the Mertens function.
Duality for normal lattice expansions and sorted residuated frames with relations
Authors: Hartonas Chrysafis 
Published: January 2023

AbstractWe revisit the problem of Stone duality for lattices with quasioperators, presenting a fresh duality result. The new result is an improvement over that of our previous work in two important respects. First, the axiomatization of frames is now simplified, partly by incorporating Gehrke’s proposal of section stability for relations. Second, morphisms are redefined so as to preserve Galois stable (and co-stable) sets and we rely for this, partly again, on Goldblatt’s recently proposed definition of bounded morphisms for polarities. In studying the dual algebraic structures associated to polarities with relations we demonstrate that stable/co-stable set operators result as the Galois closure of the restriction of classical (though sorted) image operators generated by the frame relations to Galois stable/co-stable sets. This provides a proof, at the representation level, that non-distributive logics can be regarded as fragments of sorted residuated (poly)modal logics, a research direction recently initiated by this author.
What makes a Stone topological algebra Profinite
Authors: Almeida Jorge , Goulet-Ouellet Herman , Klíma Ondřej 
Published: January 2023

AbstractThis paper is a contribution to understanding what properties should a topological algebra on a Stone space satisfy to be profinite. We reformulate and simplify proofs for some known properties using syntactic congruences. We also clarify the role of various alternative ways of describing syntactic congruences, namely by finite sets of terms and by compact sets of continuous self mappings of the algebra.
Coloring Bipartite Graphs with Semi-small List Size
Authors: Zhu Daniel G. 
Published: January 2023

AbstractRecently, Alon, Cambie, and Kang introduced asymmetric list coloring of bipartite graphs, where the size of each vertex’s list depends on its part. For complete bipartite graphs, we fix the list sizes of one part and consider the resulting asymptotics, revealing an invariant quantity instrumental in determining choosability across most of the parameter space. By connecting this quantity to a simple question on independent sets of hypergraphs, we strengthen bounds when a part has list size 2. Finally, we state via our framework a conjecture on general bipartite graphs, unifying three conjectures of Alon–Cambie–Kang.
Hotelling tubes, confidence bands and conformal inference
Authors: Koenker Roger 
Published: January 2023

AbstractStochastic frontier models and methods as pioneered by Peter Schmidt in Aigner et al. (J Econom 6:21–37, 1977), Horrace and Schmidt (J Product Anal 7:257–282, 1996), Amsler et al. (J Econom 190:280–288, 2016) constitute a rare departure from the usual econometric obsession with models for conditional means. They also provided an early stimulus for the development of quantile regression methods. After a brief tutorial on Hotelling tube methods for constructing confidence bands for nonparametric quantile regression, strengthened performance guarantees for such bands are described based on recent developments in conformal inference. These methods may be considered to be a rather idiosyncratic new approach to nonparametric inference for stochastic frontier models.