bluuebunny/crossref_metadata_2025_split huggingface dataset:
Task shifting of medical office works: A preliminary questionnaire survey for generalists
Authors: Otsuka Yuki , Hagiya Hideharu , Sunada Naruhiko , Otsuka Fumio 
Published: July 2023

AbstractTo reduce physician burden, task shifting of clerical work from physicians to medical assistants is being promoted; however, it depends on hospitals. A questionnaire survey was conducted among 40 general physicians at Okayama University Hospital in December 2022 to investigate physicians' preferences toward task shifting. Compared to other tasks, most physicians thought that ordering examinations (14, 47%), replying to referral letters (19, 63%), and prescriptions (21, 70%) and medical record entries (22, 73%) should not be task shifted. Physicians' controversial opinions on task shifting maybe the reason behind the slow progress in task shifting.
Differential growth of the adductor muscles, eyeball, and brain in the chick Gallus gallus with comments on the fossil record of stem‐group birds
Authors: Cerio Donald G. , Llera Martín Catherine J. , Hogan Aneila V. C. , Balanoff Amy M. , Watanabe Akinobu , Bever Gabriel S. 
Published: July 2023

AbstractThe avian head is unique among living reptiles in its combination of relatively large brain and eyes, coupled with relatively small adductor jaw muscles. These derived proportions lend themselves to a trade‐off hypothesis, wherein adductor size was reduced over evolutionary time as a means (or as a consequence) of neurosensory expansion. In this study, we examine this evolutionary hypothesis through the lens of development by describing the jaw‐adductor anatomy of developing chickens, Gallus gallus, and comparing the volumetric expansion of these developing muscles with growth trajectories of the brain and eye. Under the trade‐off hypothesis, we predicted that the jaw muscles would grow with negative allometry relative to brain and eyes, and that osteological signatures of a relatively large adductor system, as found in most nonavian dinosaurs, would be differentially expressed in younger chicks. Results did not meet these expectations, at least not generally, with muscle growth exhibiting positive allometry relative to that of brain and eye. We propose three, nonmutually exclusive explanations: (1) these systems do not compete for space, (2) these systems competed for space in the evolutionary past, and growth of the jaw muscles was truncated early in development (paedomorphosis), and (3) trade‐offs in developmental investment in these systems are limited temporally to the perinatal period. These explanations are considered in light of the fossil record, and most notably the skull of the stem bird Ichthyornis, which exhibits an interesting combination of plesiomorphically large adductor chamber and apomorphically large brain.
Redirecting the JAK‐STAT signal blocks the SARS‐CoV‐2 replication
Authors: Augustine George , Sisila Valappil , Indhu Mohan , Gupta Divya , Tandel Dixit , Harshan Krishnan Harinivas , Shanmugam Ganesh , Padmapriya Padmanabhan , Sivasubramanian Srinivasan , Kaveri Krishnaswamy , Ramudu Kamini Numbi , Ayyadurai Niraikulam 
Published: July 2023

AbstractThe distinct disease progression patterns of severe acute respiratory syndrome coronavirus clade 2 (SARS‐CoV‐2) indicate diverse host immune responses. SARS‐CoV‐2 severely impairs type I interferon (IFN) cell signaling, resulting in uncontrolled late‐phase lung damage in patients. For better pharmacological properties, cytokine modifications may sometimes result in a loss of biological activity against the virus. Here, we employed the genetic code expansion and engineered IFN‐β, a phase II clinical cytokine with 3‐amino tyrosine (IFN‐β‐A) that reactivates STAT2 expression in virus‐infected human cells through JAK/STAT cell signaling without affecting signal activation and serum half‐life. This study identified that genetically encoded IFN‐β‐A might stabilize the protein‐receptor complex and trigger JAK‐STAT cell signaling, which is a promising modality for controlling SARS‐CoV‐2 infection.
Comparison of Si‐GA‐PLS and Si‐CARS‐PLS build algorithms for quantitation of total polyphenols in black tea using the spectral analytical system
Authors: Zareef Muhammad , Arslan Muhammad , Hassan Md Mehedi , Ahmad Waqas , Chen Quansheng 
Published: July 2023

AbstractBACKGROUNDThe objective of the current study was to compare two machine learning approaches for the quantification of total polyphenols by choosing the optimal spectral intervals utilizing the synergy interval partial least squares (Si‐PLS) model. To increase the resilience of built models, the genetic algorithm (GA) and competitive adaptive reweighted sampling (CARS) were applied to a subset of variables.RESULTSThe collected spectral data were divided into 19 sub‐interval selections totaling 246 variables, yielding the lowest root mean square error of cross‐validation (RMSECV). The performance of the model was evaluated using the correlation coefficient for calibration (RC), prediction (RP), RMSECV, root mean square error of prediction (RMSEP) and residual predictive deviation (RPD) value. The Si‐GA‐PLS model produced the following results: PCs = 9; RC = 0.915; RMSECV = 1.39; RP = 0.8878; RMSEP = 1.62; and RPD = 2.32. The performance of the Si‐CARS‐PLS model was noted to be best at PCs = 10, while RC = 0.9723, RMSECV = 0.81, RP = 0.9114, RMSEP = 1.45 and RPD = 2.59.CONCLUSIONThe build model's prediction ability was amended in the order PLS < Si‐PLS < CARS‐PLS when full spectroscopic data were used and Si‐PLS < Si‐GA‐PLS < Si‐CARS‐PLS when interval selection was performed with the Si‐PLS model. Finally, the developed method was successfully used to quantify total polyphenols in tea. © 2023 Society of Chemical Industry.
Behavioral state‐specific resource selection of northern bobwhite chicks
Authors: Hill Justin N. , Terhune Theron M. , Martin James A. 
Published: July 2023

AbstractDetermining resource selection rates across life stages of a species is important to enable a comprehensive management approach that focuses on bolstering populations. Moreover, tying these selection rates to specific life‐history needs (e.g., foraging, roosting, loafing) can provide information for management practices. We studied northern bobwhite (Colinus virginianus) chick resource selection on a private property in Brunswick County, North Carolina, USA, to better understand foraging and roosting habitat selection. We tested 2 hypotheses (resource selection is influenced by predator avoidance, resource selection is influenced by food availability) to understand what influences specific resource use. The site was managed intensively for bobwhites using prescribed fire, seasonal mowing and disking, meso‐mammal control, and supplemental feeding. We radio‐tagged 156 chicks between 11–15 days of age from 29 unique brood captures and collected location data of roost sites and daily movement tracks for individual chicks from 2018–2019. We modeled roosting and foraging habitat selection using mixed‐effects conditional logistic regression models under a Bayesian framework. Burned upland pine (Pinus spp.) stands were selected by chicks for foraging and roosting sites. Fallow fields, unburned uplands (1 yr post‐burn), and areas closer to supplemental feed were selected by chicks as foraging habitat. We observed a negative interaction between age and fallow field selection as roosting habitat and age and areas closer to supplemental feed as foraging habitat. Our study results shed light on how circadian habitat use can vary and illustrate that one specific land cover type may not provide ideal diurnal and nocturnal habitat for bobwhite chicks.
Branding with music: How can music contour and tonality enhance perceived brand innovativeness and brand evaluations?
Authors: Zoghaib Alice , Luffarelli Jonathan , Feiereisen Stéphanie 
Published: July 2023

AbstractA large and growing number of brands use music in their marketing communications to influence consumer perceptions. This article offers an answer to how brands can leverage the characteristics of music pieces to convey an innovative image and boost brand evaluations among consumers. The authors bridge prior research in psychology, marketing, and musicology to demonstrate that using music pieces with an irregular contour or unstable tonality in communications can make brands appear more innovative. The difficulty that consumers experience in cognitively processing music pieces with these characteristics (disfluency) mediates these effects. Additionally, this research shows that branding with music that has an irregular contour or unstable tonality can either positively or negatively impact brand evaluations. This is contingent on whether brand communication includes information about brand innovativeness or brand liking. This article identifies the characteristics of music that influence perceived brand innovativeness and brand evaluations and offers a theoretical lens for understanding these effects. It also reveals which brands can benefit from using music pieces with an irregular contour or unstable tonality, as well as when they can benefit from it. Additionally, it provides practical recommendations for music selection.
Multidisciplinary molecular consultation increases the diagnosis of pediatric epileptic encephalopathy and neurodevelopmental disorders
Authors: Zhang Liping , Li Xu‐Ying , Xu Fanxi , Gao Lehong , Wang Zhanjun , Wang Xianling , Li Xian , Liu Mengyu , Zhu Junge , Yao Tingyan , Ye Jing , Qi Xiao‐Hong , Wang Yaqing , Zhao Guoguang , Wang Chaodong ,   The Xuanwu Molecular Counselling Group for Neurogenetic Diseases
Published: July 2023

AbstractBackgroundEpilepsy (EP) is a common neurological disease in which 70–80% are thought to have a genetic cause. In patients with epilepsy, neurodevelopmental delay (NDD) was prevalent. Next generation of sequencing has been widely used in diagnosing EP/NDD. However, the diagnostic yield remains to be 40%–50%. Many reanalysis pipelines and software have been developed for automated reanalysis and decision making for the diseases. Nevertheless, it is a highly challenging task for smaller genetic centers or a routine pediatric practice. To address the clinical and genetic “diagnostic odyssey,” we organized a Multidisciplinary Molecular Consultation (MMC) team for molecular consultation for 202 children with EP/NDD patients referred by lower level hospitals.MethodsAll the patients had undergone an aligned and sequential consultations and discussions by a “triple reanalysis” procedure by clinical, genetic specialists, and researchers.ResultsAmong the 202 cases for MMC, we totally identified 47 cases (23%) harboring causative variants in 24 genes and 15 chromosomal regions after the MMC. In the 15 cases with positive CNVs, 3 cases harbor the deletions or duplications in 16p11.2, and 2 cases for 1p36. The bioinformatical reanalysis revealed 47 positive cases, in which 12 (26%) were reported to be negative, VUS or incorrectly positive in pre‐MMC reports. Additionally, among 87 cases with negative cases, 4 (5%) were reported to be positive in pre‐MMC reports.ConclusionWe established a workflow allowing for a “one‐stop” collaborative assessments by experts of multiple fields and helps for correct the diagnosis of cases with falsenegative and −positive and VUS genetic reports and may have significant influences for intervention, prevention and genetic counseling of pediatric epilepsy and neurodevelopmental disorders.
Compound heterozygous variants in CFTR with potentially reducing ATP‐binding ability identified in Chinese infertile brothers with isolated congenital bilateral absence of vas deferens
Authors: Shengjia Shi , Lei Wang , Tianwei Wang , Hongmei Wang , Juanzi Shi , Sen Qiao 
Published: July 2023

AbstractBackgroundIsolated congenital bilateral absence of vas deferens (iCBAVD) in men results in obstructive azoospermia and is mainly caused by pathogenic variants in cystic fibrosis transmembrane conductance regulator (CFTR) or adhesion G protein‐coupled receptor G2 (ADGRG2).MethodsThe next‐generation sequencing (NGS) was used to screen the mutations in the proband, and Sanger sequencings were performed to validate the compound heterozygous variant of CFTR in his family members. Protein structure simulation was performed to discover the potential pathological mechanism.ResultsThis study reported novel compound heterozygous CFTR mutations (NM:000492.4, Intron: 5T; c.3965_3969dupTTGGG: p.R1325Gfs*5) in two brothers with obstructive azoospermia. The compound heterozygous CFTR mutations were first screened out by NGS in an infertile male patient who exhibited iCBAVD from a nonconsanguineous Chinese family. Histological analysis of the testicular biopsy from this patient revealed normal spermatogenesis and mature spermatozoa were observed in the seminiferous tubules. Surprisingly, the same compound heterozygous CFTR mutations were also observed in his brothers who also exhibited iCBAVD, with their parents being a heterozygous carrier for the mutations, as verified by Sanger sequencing. Protein structure simulation revealed that these mutations potentially led to impaired ATP‐binding ability of CFTR.ConclusionWe identified novel compound heterozygous CFTR mutations in two brothers and summarized the literature regarding CFTR mutation and male infertility. Our study may contribute to the genetic diagnosis of iCBAVD and future genetic counseling.
Microstructured optical beams with azimuthal polarization in stratified absorbent media
Authors: Lourenço‐Vittorino Grazielle de A. , Zamboni‐Rached Michel 
Published: July 2023

AbstractWe present an analytical method to achieve highly nonparaxial, azimuthally polarized structured beams that, when propagating through an absorbing stratified media, can assume in the last semi‐infinite layer approximately any desired longitudinal intensity pattern within spatial regions few times larger than the wavelength. The possibility of managing the properties of a highly nonparaxial beam under adverse conditions, such as multiple reflections in stratified structures and energy loss to the material media, may be of great importance in many different optical applications, like, trapping and micromanipulation, remote sensing, thin films, medical devices, medical therapies, and so on.
Patient perspective on barriers in type 2 diabetes self‐management: A qualitative study
Authors: Tuobenyiere Justin , Mensah Gwendolyn Patience , Korsah Kwadwo Ameyaw 
Published: July 2023

AbstractAimTo explore the perceived barriers in Type 2 Diabetes care among patients with diabetes. Design The study adopted a qualitative exploratory‐descriptive design.MethodsA semi‐structured interview guide was used to collect data from fifteen (15) purposively sampled patients with Type 2 Diabetes at a primary level health facility in the Bono East region. Participants' ages ranged between 42–72 years. The interviews were audio‐recorded, transcribed verbatim and analysed using thematic content analysis.ResultsPatients with Type 2 Diabetes encountered a range of barriers in diabetes care. These barriers included lack of knowledge of diabetes dietary management strategies, financial constraints, non‐compliance to treatment, lack of glucometers, lack of social support, and increased waiting time at health care facilities. The findings indicate that more education on diabetes and dietary management is required as well as social support from peers, family, and non‐governmental organizations.Patient or public contributionDiabetes self‐management barriers as revealed by the patients who were participants of this study requires nurses and midwives to ensure that self‐management education is well understood by patients and their relatives. This would empower the patients and bring clarity to their confusion about self‐care practices.
Children's Oncology Group blueprint for research: Acute lymphoblastic leukemia
Authors: Raetz Elizabeth A. , Bhojwani Deepa , Devidas Meenakshi , Gore Lia , Rabin Karen R. , Tasian Sarah K. , Teachey David T. , Loh Mignon L. 
Published: July 2023

AbstractCure rates for acute lymphoblastic leukemia (ALL), the most common childhood cancer have steadily improved over the past five decades. This is due to intensifying systemic therapy, recognizing and treating the central nervous system as a sanctuary site, and implementing modern risk stratification to deliver varying intensities of therapy based on age, presenting white blood count, sentinel somatic genetics, and therapy response. Recently, numerous Children's Oncology Group trials have demonstrated the lack of benefit of intensifying traditional chemotherapy, providing evidence that new approaches are needed to cure the patients for whom cure has been elusive. Distinguishing those who require intensive or novel therapeutic approaches from others who will be cured with minimal therapy is key for future trials. Incorporating new genomic biomarkers and more sensitive measures of minimal/measurable residual disease provide opportunities to achieve these goals.
Application of effect‐directed analysis using TLC–bioautography for rapid isolation and identification of antidiabetic compounds from the leaves of Annona cherimola Mill.
Authors: Galarce‐Bustos Oscar , Obregón Camilo , Vallejos‐Almirall Alejandro , Folch Christian , Acevedo Francisca 
Published: July 2023

AbstractIntroductionType 2 diabetes mellitus is a globally prevalent chronic disease characterised by hyperglycaemia and oxidative stress. The search for new natural bioactive compounds that contribute to controlling this condition and the application of analytical methodologies that facilitate rapid detection and identification are important challenges for science. Annona cherimola Mill. is an important source of aporphine alkaloids with many bioactivities.ObjectiveThe aim of this study is to isolate and identify antidiabetic compounds from alkaloid extracts with α‐glucosidase and α‐amylase inhibitory activity from A. cherimola Mill. leaves using an effect‐directed analysis by thin‐layer chromatography (TLC)–bioautography.MethodologyGuided fractionation for α‐glucosidase and α‐amylase inhibitors in leaf extracts was done using TLC–bioassays. The micro‐preparative TLC was used to isolate the active compounds, and the identification was performed by mass spectrometry associated with web‐based molecular networks. Additionally, in vitro estimation of the inhibitory activity and antioxidant capacity was performed in the isolated compounds.ResultsFive alkaloids (liriodenine, dicentrinone, N‐methylnuciferine, anonaine, and moupinamide) and two non‐alkaloid compounds (3‐methoxybenzenepropanoic acid and methylferulate) with inhibitory activity were isolated and identified using a combination of simple methodologies. Anonaine, moupinamide, and methylferulate showed promising results with an outstanding inhibitory activity against both enzymes and antioxidant capacity that could contribute to controlling redox imbalance.ConclusionsThese high‐throughput methodologies enabled a rapid isolation and identification of seven compounds with potential antidiabetic activity. To our knowledge, the estimated inhibitory activity of dicentrinone, N‐methylnuciferine, and anonaine against α‐glucosidase and α‐amylase is reported here for the first time.
Prenatal detection of congenital heart disease: Recent experience across the state of Arizona
Authors: Mattia Donald , Matney Chelsea , Loeb Sophie , Neale Morgan , Lindblade Christopher , Scheller McLaughlin Ericka , Rao Rashmi 
Published: July 2023

AbstractObjectiveTo determine the prenatal detection rate (PDR) of congenital heart disease (CHD) in Arizona as well as describe various factors that may influence detection rates.MethodsThis was a retrospective chart review using the Society of Thoracic Surgeons and Phoenix Children's Fetal Cardiology databases. We included all cases of CHD requiring surgery <1 year of age between 2013 and 2018. A total of 1137 patients met the criteria, and various demographic, socioeconomic, and patient outcome data were collected.ResultsThe overall PDR was 58% with an improving detection rate over the course of our study, with the final year having a PDR of 67%. Over time, PDR improved in urban communities, but this was not seen in rural communities. Rural address, public insurance, and Native American ethnicity were associated with lower PDR. Postnatal outcomes, including Apgars, initial pH, and lactate, did not differ with the presence of a prenatal diagnosis. Diagnoses typically identified with the outflow tract and 3‐vessel views on the fetal echocardiogram were less likely to be detected prenatally.ConclusionsThe PDR of CHD continues to improve with evolving technologies and guidelines. We highlight a discrepancy between urban, rural, and Native American populations. Additionally, by supplying descriptors of missed diagnosis and associated echocardiography views, we hope to provide data for future interventions.
Amphiphilic star copolymers composed of curcumin core for bacterial inactivation
Authors: Santos Madson RE , Fidelis Clara LB , Zandonadi Flávia S , Giacomelli Fernando C , Felisberti Maria Isabel 
Published: July 2023

AbstractPolymeric materials with antimicrobial properties hold the potential to be the next generation of therapeutics. This work reports the preparation of amphiphilic and star‐like copolymers through arm‐first and reversible addition–fragmentation chain transfer polymerization. The copolymers composed of poly(dimethylamine ethyl methacrylate) (PDMAEMA) as the arms and poly(curcumin dimethacrylate) (PCDMA) as the crosslinked core presented a well‐controlled amphiphilic structure with low dispersity values (Ð = 1.15). Varying the [CDMA]:[PDMAEMA] molar ratios (4, 6 and 8) led to a significant increase in the molar mass (153, 688 and 1550 kDa), particle size (33, 90 and 105 nm) and the average number of PDMAEMA arms anchored to the curcumin‐based core (12, 52 and 125 arms), as determined by dynamic and static light scattering. The antimicrobial activity of cationic PDMAEMA40‐co‐PCDMAcore was tested against Escherichia coli in the absence of light and under white light to investigate the photodynamic activity of the copolymers. The results demonstrated a concentration dependence but no additional effect under light irradiation. SEM imaging and live/dead assays revealed extensive membrane damage, evidencing a membrane‐disruption mechanism. HeLa viability assays demonstrated that at 1 mg mL−1, 80 ± 10% of the cells demonstrated metabolic activity after 24 h. © 2023 Society of Industrial Chemistry.
Towards deciphering glioblastoma intra‐tumoral heterogeneity: The importance of integrating multidimensional models
Authors: Sajid Rifat Shahriar , van Winden Lennart J. , Diamandis Phedias 
Published: July 2023

AbstractGlioblastoma (GBM) is the most common and severe form of brain cancer among adults. Its aggressiveness is largely attributed to its complex and heterogeneous biology that despite maximal surgery and multimodal chemoradiation treatment, inevitably recurs. Traditional large‐scale profiling approaches have contributed substantially to the understanding of patient‐to‐patient inter‐tumoral differences in GBM. However, it is now clear that biological differences within an individual (intra‐tumoral heterogeneity) are also a prominent factor in treatment resistance and recurrence of GBM and will likely require integration of data from multiple recently developed omics platforms to fully unravel. Here we dissect the growing geospatial model of GBM, which layers intra‐tumoral heterogeneity on a GBM stem cell (GSC) precursor, single cell, and spatial level. We discuss potential unique and inter‐dependant aspects of the model including potential discordances between observed genotypes and phenotypes in GBM.