bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiomics reveals the role of macrophage-vascular endothelial cell interactions in the hepatoblastoma tumor microenvironment
Authors: Zhu Qian , Xie Yuwei , Qiu Kang , Wu Tingting , Zhang Peng , Sun Xinxin , Wang Hongli , Ji Ming , Hao Xiwei , Zhu Chengzhan 
Published: July 2023

Abstract
Background
Hepatoblastoma is a common tumor in childhood, characterized by immature histology and diverse cell lineages. The purpose of this study is to identify the genes that are abnormally expressed in hepatoblastoma, and to explore and verify the targets of intercellular communication that affect the tumor immune microenvironment.
Methods
Through comprehensive analysis of gene expression from GSE133039 and GSE180664 data sets, the differentially expressed genes in cancer tissues and adjacent tissues were obtained. GO and KEGG enrichment analysis is used to predict the biological function and signal transduction pathway of differential expression gene enrichment. Use cytoscape to build PPI network to filter hubgene; Construct correlation analysis of immune cell infiltration to infer the correlation between immune cells. Combined with single-cell transcriptome data, further reveal the relationship between cells and signal targets of cell communication.
Results
58 differentially expressed genes with high expression and 94 differentially expressed genes with low expression were obtained from the two data sets of hepatoblastoma. They were mainly involved in the signal transduction related to metastasis. PPI network screened 50 hubgenes. The correlation analysis of immune cell infiltration of different genes showed that macrophages were significantly correlated with endothelial cells. Combined with the analysis of single-cell transcriptome data, hepatoblastoma was divided into 11 cell subpopulations, and 16 genes in hubgene were expressed in different cell subpopulations, in which LFNG was highly expressed in macrophages and monocytes, which served as the target of intercellular communication to promote the development of hepatoblastoma.
Conclusion
In this study, we identified the genes that were abnormally expressed in hepatoblastoma, and affected the cellular communication of hepatoblastoma through LFNG target, thus affecting the progress of tumor. Therefore, LFNG may become a therapeutic target for hepatoblastoma.
Home Range, Site Fidelity, and Movements of the Timber Rattlesnake (Crotalus horridus) in West-Central Illinois
Authors: Eckert Scott A. , Jesper Andrew C. 
Published: July 2023

Abstract
Understanding the home range of reptiles is important to their conservation and recovery efforts. Despite numerous home range studies for the imperiled timber rattlesnake (Crotalus horridus), only a single study has been undertaken in the central midwestern United States. We report on the home range size, site fidelity, and movements of this species in west-central Illinois. Using VHF telemetry, we located 29 C. horridus (13 female, 16 male) every 1.3 days over a 5-year period for 51 years ofrecords of the species' locations and movements. We calculated annual home ranges for each snake per year using 99%, 95%, and 50% isopleths derived from Brownian Bridge utilization distributions, and we also report 100% minimum convex polygons for comparison with other studies. We examined the effects of sex, mass, SVL, and year on home range sizes and reportedon movement metrics as well as home range fidelity using both UDOI and BA statistics. The homerange sizes for male and non-gravid C. horridus were 56.91 Ha (CI = 41.04-72.77) and 20.22 (CI = 3.81-36.62) for 95% BBMM; 7.56 Ha (CI = 5.47-9.65) and 2.36 Ha (CI = 0.19-4.53) for 50% BBMM; and 80.03 Ha (CI = 50.95-109.11) and 30.80 Ha (CI = 1.55-60.06) for MCP. The estimated daily distance traveled was significantly greater for males (mean = 57.25 m/day, CI=49.06-65.43) than females (mean = 27.55 m/day, CI = 18.99-36.12). Similarly, maximum displacement distances from hibernacula were significantly greater for males (mean = 2.03 km, CI = 1.57-2.48) than females (mean = 1.29 km, CI = 0.85-1.73], and on average, males were located further from their hibernacula throughout the entirety of their active season. We calculated habitat fidelity using 11 snakes that were tracked over multiple years. The mean BBMM overlap using Bhattacharyya's Affinity (BA) for all snakes at the 99%, 95%, and 50% isopleths was 0.46 (SE=0.03), 0.38 (SE=0.03), and 0.08 (SE=0.02), respectively. The mean BBMM overlap for all snakes using the Utilization Distribution Overlap Index (UDOI) at the 99%, 95%, and 50% isopleths was 0.78 (SE=0.13), 0.41 (SE=0.03), and 0.09 (0.02), respectively. Our results are largely consistent with those of other studies in terms of home range sizes and theinfluence of sex on those home ranges and distances traveled. The species also exhibits strong site fidelity with snakes generally using the same habitats each summer. Particularly interesting was the tendency for snakes to disperse from specific hibernacula in the same general direction to the same general areas. We propose some possible reasons for this dispersal pattern.
Effectiveness of Endoscopic Screening in Reducing Esophageal Cancer Mortality: A Nested Case-control Study
Authors: LU Youhua , Qi Yuantao , MA Siqi , YANG Jia , ZHAO Li , ZHAO Deli , MA Guifeng , ZHANG Nan , MA Anning , WANG Jialin 
Published: July 2023

Abstract
Purpose To evaluate the effectiveness of endoscopic screening in reducing esophageal cancer mortality within a high-incidence area.
Methods A nested case-control study was conducted based on the Upper Gastrointestinal Cancer Screening Program of Feicheng City. Based on the screening population cohort, individuals who were newly diagnosed with esophageal cancer from September 2006 to December 2016 and died of esophageal cancer before December 2018 as case subjects. Each case matched 4 controls (Subjects who were alive on the date of death of the corresponding case) based on age, gender and screening village. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated via conditional logistic analysis.
Results This study included 345 cases (270 men and 75 women) and 1380 matched controls (1,080 men and 300 women). Compared with individuals who were never screened, the total ORs was 0.52(CI 0.39-0.70) for participants, the OR of screened subjects 40 to 49 years old was 0.34 (CI 0.17-0.67), and the OR for dying from esophageal cancer among individuals who were diagnosed 2 to 4 years was 0.30 (CI 0.17-0.53).
Conclusions Participating in endoscopic screening could reduce the risk of death from esophageal cancer by 48%. The screening effect was related to the age of screening and the period from the screening to the date of diagnosis.
Development and validation of a machine learning model for bone metastasis in prostate cancer: Based on inflammatory and nutritional indicators
Authors: Jin Tongtong , An Jingjing , Wu Wangjian , Wang Chao , Zhou Fenghai 
Published: July 2023

Abstract

Purpose
Application of machine learning in bone metastasis of prostate cancer based on inflammation and nutritional indicators.
Methods
Retrospective analysis the clinical data of patients with prostate cancer initially diagnosed in the Department of Urology of Gansu Provincial People's Hospital from June 2017 to June 2022. Logistic regression (LR) and least absolute shrinkage and selection operator (LASSO) are used to jointly screened the model features. The filtered features are incorporated into algorithms including LR, random forest (RF), extreme gradient boosting (XGBoost), naive nayes (NB), k-nearest neighbor (KNN), and decision tree (DT), to develop prostate cancer bone metastasis models.
Results
A total of 404 patients were finally screened. Gleason score, T stage, N stage, PSA and ALP were used as features for modeling. The average AUC of the 5-fold cross-validation for each machine learning model in the training set is: LR (AUC = 0.9054), RF (AUC = 0.9032), NB (AUC = 0.8961), KNN (AUC = 0.8704), DT (AUC = 0.8526), XGBoost (AUC = 0.8066). The AUC of each machine learning model in the test set is KNN (AUC = 0.9390, 95%CI: 0.8760 ~ 1), RF (AUC = 0.9290, 95%CI: 0.8718 ~ 0.9861), NB (AUC = 0.9268, 95%CI: 0.8615 ~ 0.9920), LR (AUC = 0.9212, 95%CI: 0.8506 ~ 0.9917), XGBoost (AUC = 0.8292, 95%CI: 0.7442 ~ 0.9141), DT (AUC = 0.8057, 95%CI: 0.7100 ~ 0.9014). A comprehensive evaluation of the DeLong test among different models and each evaluation metric shows that KNN is the best machine learning model in the study.
Conclusion
A bone metastasis model of prostate cancer was established, and it was observed that indicators such as inflammation and nutrition had a weak correlation with bone metastasis.
Investigating alcohol consumption in China via Wastewater-based Epidemiology
Authors: Yao Yan , Wang Jingya , Zhong Yuling , Chen Wenyu , Rao Yulan , Su Mengxiang , Di Bin 
Published: July 2023

Abstract
Alcohol abuse and addiction remain priority public health issues worldwide. Accurate estimation of alcohol prevalence is increasingly challenging due to the time-consuming and labor-intensive nature of traditional survey methods. This study examined alcohol consumption in major cities of China via Wastewater-based epidemiology(WBE) and compared WBE estimates with other data sources. A simple and valid ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of two alcohol metabolites, ethyl glucuronide (EtG) and ethyl sulfate (EtS) in wastewater. The optimized method was applied to 62 sewage samples collected from wastewater treatment plants (WWTPs) in 31 provincial capital cities across China in the fourth quarter of 2020. The methodology established in this study was validated with the lower limit of quantification (LLOQ) up to 0.1 µg/L, good linearity in the range of 0.1–50 µg/L, intra-day and inter-day precision less than 5.58% and 5.55%, respectively, and the recoveries of the extracts were higher than 97.14%. The consumption range of alcohol estimated via WBE was 1.23 ~ 18.18 mL ethanol/person /day (EPD) in the capital cities of China. There was a significant difference in alcohol consumption among cities and WBE estimates of alcohol consumption were lower than WHO survey results and higher than foreign countries. The investigation of alcohol consumption based on WBE is very potential, which can complement objective information for estimating the consumption of alcohol.
The Ethanol-Producing Enterocloster bolteae and Limosilactobacillus fermentum are enriched in Nonalcoholic steatohepatitis
Authors: MBAYE Babacar , WASFY Reham MAGDY , BORENTAIN Patrick , TIDJANI Maryam , MOTTOLA Giovanna , BOSSI Vincent , CAPUTO Aurelia , RAOULT Didier , GEROLAMI Rene , MILLION Matthieu 
Published: July 2023

Abstract
Background: Non-alcoholic steatohepatitis (NASH) has become a major public health issue. In fact, it is one of the leading causes of disturbed liver function and liver transplantation worldwide. Its link with the intestinal microbiota is still topical, and the production of endogenous ethanol following dysbiosis of the microbiota - one of the mechanisms linked to the disease - has been shown in several studies. In this study, we analyzed stool samples from 41 NASH patients and 24 healthy controls using culturomics and 16S rRNA targeted metagenomics to identify the microbial profile associated with each group. Fecal ethanol concentration was also determined for all samples.
Results: We identified 358 different bacterial species using the culturomics approach distributed into 11 phyla and 143 genera. With a significant difference, 12 appeared significantly more frequently in the NASH group and 4 more frequently in the control group. Finegoldia magna was the most detected in NASH patients (10/14 (71%)), and 2 ethanol producing strains: Limosilactobacillus fermentum and Enterocloster bolteae.
Metagenomics shows an enrichment of Lactobacillus and Streptococcus and a microbial profile identical to that of the culture. With regard to the species, there was an elevated frequency of ethanol-producing bacteria in NASH patients, and the concentration of ethanol was also higher in the stools of NASH patients compared to healthy controls (bilateral Mann-Whitney test, p=value 0.0145). However, compared with metagenomics, only the culture showed enrichment of Enterocloster bolteae in NASH patients.
Conclusions: Culturomics and metagenomics are complementary methods for exploring the microbiota. Our results suggest that enrichment of ethanol-producing bacteria in NASH patients may play an instrumental role in the pathogenesis of the disease.
Patient-derived ex vivo cell culture growth dynamics predict recurrence probability in patients with atypical and anaplastic meningiomas
Authors: Bissolo Marco , Masalha Waseem , Scheffler Pierre , Steiert Christine , Sankowski Roman , Beck Jürgen , Schnell Oliver , Heiland Dieter Henrik , Grauvogel Jürgen 
Published: July 2023

Abstract
Purpose
The prediction of recurrence in meningiomas is limited by the lack of ex vivo meningioma models. By introducing a novel patient-derived ex vivo cell culture model, we aimed to utilize cell growth dynamics to predict recurrence patterns.
Methods
Tissue samples were collected from patients who underwent meningioma resection at our department between April 2021 and November 2021 immediately after tissue harvesting. After establishing a novel cell culture procedure in a modified DMEM medium, histopathological analysis, and immunohistochemistry were performed. Population doubling time and cell growth rate were measured during the 38-day observational period. The mean clinical follow-up was 18 months. Clinical and radiological examinations were conducted before surgery and three and 15 months after tumor resection.
Results
A patient-derived ex vivo cell culture model was successfully established for 21 of 29 samples of central nervous system-World Health Organization (WHO) grade 1–3 meningiomas. The population doubling time and two-week ex vivo model survival differed significantly among malignancy grades (p = 0.010). Viability at the second week of culture correlated with WHO grade (p = 0.024). A positive population doubling time at the second week and a population doubling time of < 200 hours were correlated with relapsing disease within 15 months (p = 0.013).
Conclusion
As early as the end of the second week of cell culture, growth dynamics may provide prognosis data for atypical and anaplastic meningiomas. Further studies of cell growth patterns may support therapeutic decisions in patients with meningiomas when molecular analysis cannot be performed.
Contribution of wastewater irrigated vegetables to the prevalence of soil- transmitted helminth infection among female farmers in Addis Ababa, Ethiopia
Authors: Gurmassa Bethelhem kinfu , Gari Sirak Robele , Solomon Ephrem Tefera , Goodson Michaela L. , Walsh Claire L. , Dessie Bitwe K. , Alemu Bezatu Mengistie 
Published: July 2023

Abstract
Background
Many farmers in and around Addis Ababa city rely on untreated wastewater for irrigation due to a growing population and limited access to reliable agricultural water sources. However, data on STH contamination of irrigated vegetables and farmer risk are scanty. This study aimed to determine the prevalence of STH in vegetables and female farmer stool samples in Ethiopia, as well as the association between helminths in vegetables and female farmers' stool.
Methods
A cross-sectional study was conducted between November 2021 and February 2022 to investigate the prevalence of helminths in vegetable samples and stool samples of female wastewater irrigated vegetable growers. A stratified random sampling technique was employed to select farming households. The sample size for each district was set by a proportionate allocation to the total number of homes. Two hundred fifty-two composite samples and 101 stool samples from the wastewater-irrigated farm were collected and analyzed. A questionnaire was utilized to collect qualitative data, while vegetable samples were used to obtain quantitative data. Stata version 14.0 was used to process the data. Using Poisson regression, the relationship between helminths in vegetable samples and farmer health was studied. Statistical significance was set at p-value < 0.05.
Result
STH were found in 67.5% of the vegetables and 20.8% of the female farmers' stools. Significant concentrations of Ascaris lumbricoides (vegetable 48.4% and stool 9.9%) were identified in all analyzed samples, followed by hookworm (vegetable 13.1% and stool 8.9%) and Trichuris trichiura (vegetable 5.9% and stool 2%). The presence of helminths in wastewater-irrigated vegetables and the level of infection in exposed farmers had a statistically significant positive association (p < 0.05).
Conclusion
Vegetables grown with wastewater irrigation are contaminated with STH, which could be a source of infections for farmers. Therefore, in addition to reducing occupational exposure, risk reduction programs should target households that have no knowledge how to prevent the spread of helminths.
Impact of Climate Variability and Environmental Policies on Vegetation Dynamics in the Semi-Arid Tigray
Authors: Tefera Amare Sisay , Siyum Zenebe Girmay , Berhe Daniel Hagos , Gebru Belay Manjur 
Published: July 2023

Abstract
Anthropogenic and climate-related phenomena are among the main factors responsible for variations in vegetation structure and composition worldwide. However, studies that integrate the effects of human activities and climate variability are still lacking in the fragile tropical ecosystems, including the semi-arid Tigray region. This study examined the relationship between vegetation cover, climate variability, and environmental policies in the semi-arid Tigray region of Ethiopia. Using satellite-based vegetation index and high-resolution rainfall data, the study found that human factors, particularly environmental policy implementations, played a more significant role in changing vegetation cover than climate variables. With r2 = 0.19, the regression analysis showed an insignificant causal relationship between vegetation dynamics and precipitation over the 41-year study period. However, the residual analysis result (r = -0.55, z-stat = -11.58, p < 0.01) indicated a strong relationship between vegetation change and environmental policies implemented within the specified study period. The research also highlighted the negative impact of the global rise in temperature on vegetation cover due to water stress caused by evapotranspiration. These findings suggest the need for future research to focus on assessing variations in correlation among different vegetation formations and policy changes in detail.
Nodular lymphoid hyperplasia, a rare case report
Authors: Liu Fangxiao , zhang cheng , liu lin , Zhao Jiaqi , Jiao zheng 
Published: July 2023

Abstract
Background: A rare immune-deficient condition is known as nodular lymphoid hyperplasia (NLH). It is not known how common it is, but gene-molecular testing in conjunction with a medical history may definitively identify it.
Case summary: A 28-year-old man who had been seeking medical care for sporadic stomach pain throughout the previous years was described in the case. Colonoscopy showed 2 to 4 mm-diameter diffuse nodular hyperplasia of the rectum and ileum mucosa. The Lamina propria had a significant infiltration of lymphocytic inflammatory cells, according to ileal and rectal biopsies.To 0.01g/L, the immunoglobulin A level had drastically dropped.Finally, supplementary testing in conjunction with a whole gene exon test was used to determine the diagnosis of nodular lymphoid histiocytosis. Regular follow-up testing is necessary since this case runs the risk of developing into intestinal lymphoma.
Conclusion: This example highlights the fact that abdominal discomfort is a frequent clinical symptom that can be caused by a variety of different conditions, and while NLH is an uncommon disease, doctors are advised not to dismiss it when it is present.
Hormigas (Hymenoptera: Formicidae) asociadas a Lecythis minor j. (Ericales: Lecythidaceae) en un relicto de bosque seco tropical, Santa Marta, Colombia
Authors: Sierra-Chamorro Hubert Andrés , Soto-Avendaño Michelle Vanesa , Castrillo Miranda Aldair de Jesús 
Published: July 2023

El bosque seco es uno de los ecosistemas estratégicos en Colombia por su alta biodiversidad y los múltiples servicios ecosistémicos que brinda. En estos ambientes se destacan especies de plantas como Lecythis minor, una lecitidácea que se distingue por su fruto en forma de olla. Estas plantas pueden establecer interacciones complejas con organismos como las hormigas, grupo de insectos que se caracteriza por sus diversidades taxonómica y funcional y por su éxito ecológico. Asimismo, estas asociaciones permiten entender procesos de coevolución complementarios a niveles ecológico y funcional. Por lo tanto, el objetivo de este trabajo fue determinar la comunidad de hormigas asociadas a la olla de mono, sus grupos funcionales y las posibles interacciones en un relicto de bosque seco tropical en la Universidad del Magdalena. Para ello, se muestrearon ocho individuos en el relicto de bosque seco tropical en los que se registraron 11 especies de hormigas y cinco de sus grupos funcionales. La comunidad estuvo representada en su mayoría por formicinas generalistas y myrmicinas arbóreas; las cuales se registraron aprovechando recursos como néctar, mucilago y sitios para nidificar. Los grupos funcionales de las depredadoras epigeas y arbóreas solo estuvieron representadas por una especie. Algunas, hormigas como Crematogaster y Pseudomyrmex presentan la capacidad de brindar protección a la planta; otras, como Cephalotes, se asocian a procesos de polinización, mientras que la planta ofrece alimento y sitios para nidificar. Por su parte, las generalistas como Camponotus y Brachymyrmex aprovechan recursos de la planta sin brindar un beneficio aparente a esta. Este primer acercamiento a la comunidad de hormigas asociadas a L. minor y sus interacciones sugiere la posible mirmecofilia de la planta. Sin embargo, es necesario llevar estudios en zonas más conservadas que permitan ampliar el conocimiento de las especies de hormigas asociadas a la olla de mono.
Novel Pyrazino[2,3-b] Pyrazines as mTOR Kinase Inhibitors for
Treating Cancer and other Diseases
Authors: De Surya K. 
Published: July 2023

:
This paper describes the synthesis of some heteroaryl compounds and compositions
comprising an effective amount of one or more such compounds and methods for
treating or preventing cancer, inflammatory conditions, immunological conditions, metabolic
conditions and conditions treatable or preventable by inhibition of a kinase pathway,
comprising administering an adequate amount of a heteroaryl compound to a patient in
need thereof. These compounds are mTOR/PI3K/Akt pathway inhibitors.