bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cyclosporine A enhanced the cytotoxicity of immunotoxin to the HER-2-overexpressing SK-OV-3 cells
Authors: Xie Huilong , fu yunrong , Lin Zhisheng , Wang Jiayi , Wu Jingyu , You Xiuhua , Xie Jieming 
Published: July 2023

Abstract
Background
Cyclosporine A (CsA) is a calcium antagonist mainly used as an immune-suppressive agent, which can enhance the cytotoxicity of immunotoxins through an unknown mechanism. In this study, T-CUS245C was an immunotoxin conjugated with trastuzumab(T) and recombinant cucurmosin 245C (CUS245C), commonly used to induce apoptosis and differentiation in caner cells.
Objectives
To explore the synergistic effect of T-CUS245C combined with CsA, and to reveal it's initial mechanism.
Methods
We investigated the synergistic effect of CsA combined with T-CUS245C on cell proliferation inhibition and apoptosis in SK-OV-3 cells with HER-2-overexpressing by SRB assay and apoptosis assays. The effect of CsA on the intracellular distribution of T-CUS245C was observed by confocal microscopy and quantified by flow cytometry.
Results
The results showed that the combination therapy index (CI) of CsA combined with T-CUS245C was less than 0.7, indicating that CsA could significantly enhance the proliferation inhibition and apoptosis induced by T-CUS245C in SK-OV-3 cells. The result of confocal microscopy and flow cytometry showed that CsA could effectively increase the dispersion of FITC-T-CUS245C in the cytoplasm, suggesting that CsA might enhance the cytotoxicity of T-CUS245C by promoting the endolysosomal escape.
Conclusion
CsA might improve the cytotoxicity of T-CUS245C by facilitating the release of immunotoxin in the intracellular space, which could be a potencial strategy to transport antibody-based drugs from endolysosomes into the cytoplasm.
A comparative analysis of core needle biopsy and repeat fine needle aspiration in patients with inconclusive initial cytology of thyroid Nodules
Authors: Su Xuejiao , Yue Can , Yang Wanting , Ma BuYun 
Published: July 2023

Abstract
Purpose To assess and compare the effectiveness of ultrasound-guided core needle biopsy (CNB) in comparison to repeat fine-needle aspiration(rFNA) for thyroid nodules that yield inconclusive results following the initial fine-needle aspiration (FNA).Methods A cohort of 471 patients who received an inconclusive cytological diagnosis following the initial FNA were included in this study. These patients subsequently underwent either CNB (n = 242) or rFNA (n = 229). The inconclusive FNA results encompassed categories I, III, and IV of the TBSRTC, as well as the ultrasound images indicating malignancy despite FNA results falling under TBSRTC category II. This study assessed the sampling satisfaction rate, diagnostic efficacy, and complications associated with CNB compared to rFNA. Additionally, the impact of repeat puncture time and nodule size on diagnostic efficacy was analyzed.Results Following repeat punctures, the satisfaction rate of the CNB sampling was found to be significantly higher than that of rFNA (83.9% vs 66.8%). The diagnostic rate in the CNB group was significantly greater compared to that of the rFNA group (71.1% vs 35.8%). In patients with nodule maximum diameters ranging from 5 mm to less than 8 mm, 8 mm to less than 10 mm, and 10 mm to less than 20 mm, the diagnostic accuracy was significantly higher in the CNB group compared to that in the rFNA group. In patients with intervals less than 90 days, between 90 and 180 days, and between 180 days and one year, the diagnostic rate in the CNB group was found to be higher compared to that in the rFNA group. In CNB ,not immediately adjacent to the capsule was a risk factor for nodular puncture bleeding (37.0% vs 22.7%.)Conclusion CNB demonstrated higher rates of satisfaction and diagnosis compared to the rFNA. The diagnostic effectiveness of CNB was not influenced by the time interval or the size of the thyroid nodule. Therefore, in cases where the initial FNA diagnosis of thyroid nodules is inconclusive, CNB should be considered as a viable option for re-puncture.
The energy consumption and efficiency of crop production in China
Authors: Mu Muxiaoya , Ge Xiaodong , Cao Congge , Zeng Yanyu , Xu Tianle , Huang Jiajia , Rong Weikang 
Published: July 2023

Abstract

Energy efficiency improvement is essential to the adaptiveness of agriculture to climate change in China, especially to meet the demands for both carbon neutrality and food security. This study examines the roles of agricultural inputs in energy consumption, crop production growth, and energy efficiency improvement from a long-term perspective (1949 to 2020). The results are as follows. (1) The total energy consumption of crop production increased at various increasing rates in different phases. (2) Energy efficiency presented a downward trend, from 0.468 tons of biomass per GJ energy consumption in 1949 to 0.178 tons per GJ in 2020. The decrease in energy efficiency slowed down when the agricultural biomass output increased. (3) From 1949 to 1978, mechanization and irrigation dominated the change in energy efficiency in crop production, accounting for about 47% of total energy consumption and 30.1% of crop production growth. From 1978 to 2012, fertilizer, pesticides, and film contributed 36% of total energy consumption and 6.0% of crop biomass growth. From 2012 to 2020, as fertilizer and pesticides were limited in crop production. Mechanization dominated the change in energy efficiency, accounting for about 18.7% of total energy consumption and 18.2% of crop production growth. (4) There were still challenges and uncertainties in agricultural energy efficiency improvement. The most important influencing factors include integrating energy efficiency and food security goals, rational allocation of the agricultural inputs, and further research and development of technologies for green agriculture innovation.
Research on the Z.S. technique for 8-0 polypropylene suture in scleral fixation of one-piece intraocular lens
Authors: Si Shancheng , Zhang Liu , Yang Rong , Li Yanhong , Zhang Jianhui 
Published: July 2023

Abstract
Purpose
To introduce a new scleral flapless knotless interlaminar anchoring technique (which we called Z.S. scleral fixation technique) for 8 − 0 polypropylene suture in fixation of intraocular lens (IOL) and evaluate its efficacy and safety.
SETTING:
Department of Glaucoma, Fuzhou Eye Hospital, 350007, Fuzhou, China
DESIGN:
Retrospective case series.
Methods
All surgeries were performed according to two strategies. At first, the Z.S. scleral fixation technique for 8 − 0 polypropylene suture in fixation of one-piece IOL was used to fix suture to sclera. And then, the one-piece IOL previously implanted in the anterior chamber was relocated to the posterior chamber. Finally, the suture ends outside the sclera were also ironed into the shape of a ball and buried in the sclera. All effectiveness and safety data related to the Z.S. technique was recorded and compared.
Results
The Z.S. scleral fixation technique was employed in 16 eyes of 13 patients, with the follow-up duration of at least 6 months. Postoperative uncorrected distance visual acuity (UDVA) was greatly improved in most of the operated eyes (11/16). The mean log MAR UDVA after surgery was significantly improved compared with that before surgery (0.93 ± 0.72 vs 1.53 ± 0.73, P = 0.002). IOLs in the vast majority of operated eyes (15/16) remained stable during half-year follow-up. No severe intra-/post- operative complications were observed in any of the operated eyes.
Conclusion
The Z.S. scleral fixation technique for 8 − 0 polypropylene suture in fixation of one-piece IOL reduced the surgical injuries and had ideal surgical efficacy and safety.
Central Attention with Multi-graphs for Image Annotation
Authors: Liu Baodi , Shao Qianqian , Liu Yan , Liu Weifeng 
Published: July 2023

Abstract
Automatic image annotation has exerted a tremendous fascination on researchers in recent decades due to the development of multimedia and computer vision. Much of the research only exercise the single graph to annotate the images in semi-supervised learning. And a multitude of methods examine how to use multi-view or image segmentation to compose multiple graph structures. However, the single graph is arduous to express the complete manifold structure information. Further, it is structurally computationally intensive and consumes a lot of time that building multiple graph structures depending on multiview or image segmentation. To this end, we propose a method, called Central Attention with Multi-graphs for Image Annotation. We firstly propose that the center part of an image is fatal for the image. And the promising performance can be accomplished relying solely on the two graph structures composed by central and overall features in semi-supervised learning. A number of experiments have been conducted on benchmark datasets, such as Corel5K, ESPGame, and IAPRTC12 to prove the effectiveness of the proposed method.
ACT001 inhibits tumor progression and reduces the expression of PD-L1 in non-small cell lung cancer
Authors: Li Yiman , Song Yan , Guo Hui , He Jinling , Hou Huijie , Shi Minmin , Shi Zhijing , Pan Fei , Ji Jinlu , Hu JunQi , Sun Zhijia , Chen Zhe 
Published: July 2023

Abstract
Purpose In recent years, remarkable advances in the therapeutic strategies for Non–small cell lung cancer (NSCLC) have revolutionized the treatment of NSCLC. However, in view of the low immune response, secondary drug resistance, autoimmune side effects, and financial burden of immunotherapy, developing novel immunotherapy or small-molecule compound is urged. Thus, this study investigates the effects of ACT001, a novel small-molecule inhibitor, on the antitumoral and immunomodulatory effects of ACT001 in NSCLC and explores the underlying mechanism.Methods The effects of ACT001 on NSCLC cells proliferation, clone formation, apoptosis, migration and invasion were measured using CCK8, clone formation, EDU stains, flow cytometry, transwell, and invasion assays, respectively. Flow cytometry was used to detect apoptosis of tumor cells and expression of granzyme B in CD3+ T cells in the co-culture system. Pull-down and mass spectrometry analysis were used to screen the interacting proteins of ACT001. Transcription factor binding sites were predicted by JASPAR database. Western blotting, rt-PCR, and flow cytometry were used to detect PD-L1, p-STAT1, and p-STAT3 expression in NSCLC cells exposed to ACT001.Results ACT001 inhibited the proliferation, migration, invasion and cell cycle progression of NSCLC cells, while promoted NSCLC cell apoptosis. ACT001 could decrease the expression of PD-L1 in NSCLC cells by inhibiting the phosphorylation of STAT1 and STAT3, which directly bind to the promoter of PD-L. Furthermore, ACT001 enhanced the release of granzyme B in CD3+ T cells, and promoted the apoptosis of NSCLC cells in the co-culture system.Conclusion We discovered that ACT001 exhibits a dual effect in NSCLC. On one hand, ACT001 has direct cytotoxicity on tumor cells in terms of inhibiting the proliferation, migration and invasion, and promoting NSCLC cell apoptosis. On the other hand, it also leads downregulation of PD-L1 in NSCLC cells to restore the anti-tumor ability of T cells. Our findings provide novel insights into the antitumoral and immunomodulatory effects of ACT001 which may shed new light on treatment of NSCLC.
Uncovering the mechanism of selective stabilization of high-energy stereoisomers via inclusion
Authors: Oakley Meagan , Oakes Madaline , Wagner Brian , Pearson Jason Kenneth 
Published: July 2023

Abstract
Supramolecular systems may be used to stabilize otherwise unstable isomers to find alternative synthetic pathways. It has been reported that cucurbit[8]uril can stabilize trans-I and trans-II CuIII cyclam whereas trans-III is the only non-substituted trans CuII cyclam stereoisomer found outside of the host molecule experimentally. Quantum chemical calculations can provide valuable insight into the intermolecular interactions involved in these inclusion complexes. All five possible trans stereoisomers of CuII cyclam were studied within the host to compute the interaction energy and free energy of association for each complex. The relative free energies of the five free cyclams confirm that trans-I and trans-II are the most energetically accessible stereoisomers from the initial trans-III starting point. Energy decomposition analysis was used to identify the attractive and repulsive interactions between cyclam and cucurbit[8]uril and showed that trans-II encounters repulsive forces almost three times greater than trans-I, which may explain the 7:3 ratio of trans-I to trans-II that occurs experimentally. Optimized complex geometries with trans-III, IV, and V show that the cyclams protrude out of cucurbit[8]uril, whereas trans-I and trans-II become more encapsulated and elongate the host, suggesting that the position of the cyclam is extremely important when forming non-covalent interactions. Our results agree with the experimental findings and provide greater insight into why the most stable isolated cyclam stereoisomer, trans-III, does not form a complex.
YY1-regulated AEBP1 drives the progression and inhibits ferroptosis of lung adenocarcinoma
Authors: Gao Qiang , Li Jie 
Published: July 2023

Abstract
Lung adenocarcinoma (LUAD) is the most common and severe type of lung cancer and it is characterized by high morbidity and mortality rates. Adipocyte enhancer binding protein 1 (AEBP1), a cancer-promoting factor in several types of cancer, plays a significant regulatory role in cancer. However, the regulatory mechanism underlying the effect of AEBP1 on LUAD has not been previously investigated. Therefore, in the present study the mRNA and protein expression levels of AEBP1 were detected in LUAD cells by reverse transcription-quantitative PCR and western blot analysis, respectively. Furthermore, AEBP1 was silenced in LUAD cells using the cell transfection technology and Cell Counting Kit-8, EdU, clonogenesis, wound healing, Transwell and tube formation assays were performed to assess cell proliferation, invasion, migration and angiogenesis. Subsequently, thiobarbituric acid-reactive substances production rate and Fe2+ levels were measured using the corresponding kits. Reactive oxygen species levels were detected by BODIPY (581/591) C11 staining, while the effect of AEBP1 silencing on ferroptosis was evaluated via detecting the expression levels of ferroptosis-related proteins by western blot analysis. The association between AEBP1 and Yin Yang 1 (YY1) was verified by luciferase reporter gene and chromatin immunoprecipitation assays. To further uncover the regulatory mechanism of AEBP1, cells were simultaneously transfected with AEBP1 knockdown and YY1overexpression plasmids and the relevant indicators of cell proliferation, invasion, migration, angiogenesis and ferroptosis were assessed. The results showed that AEBP1 silencing inhibited the proliferation, metastasis and angiogenesis of LUAD cells. Additionally, AEBP1 knockdown induced ferroptosis in LUAD cells. The above regulatory effects of AEBP1 were triggered via regulating YY1 transcription factor. Overall, the current study suggested that YY1-regulated AEBP1 could promote the progression of LUAD and inhibit ferroptosis.
Seismic Performance of 3Dimensional Interior Reinforced Concrete Beam-Column Joints Strengthened with CFRP sheets
Authors: Farahbod Farhang , Aalam Hamid , Jahanmohammadi Atefeh 
Published: July 2023

Abstract
Recent studies on flexural/shear strengthening of reinforced concrete beam-column joints with FRP composites have mainly focused on rehabilitation of 2Dimensional connections regardless of the effects of perpendicular beams. Present experimental study investigates the behavior of 3Dimensional interior RC joint under simultaneous lateral reversed cyclic loading in one direction and gravity loads in the orthogonal orientation. Three full-scale specimens were tested including one un-strengthened and two strengthened ones. Strengthening was performed using 1 and 3 L-shape layers of CFRP composite sheets at top and bottom faces of the joints, 1- and 4-strip wrapping layers at beams,1 and 2 full wrapping layers at column and conducting the method of externally bonded reinforcement on grooves (EBROG) to prohibit the premature FRP debonding. Results for failure mode, flexural capacity, ductility factor, energy dissipation and stiffness degradation indicate improvements in seismic behavior of flexural strengthened joints, where specially the increase in strength was reported 9 and 23 percents for two strengthened specimens. To evaluate the reported information, “sectional analysis theory” has been adopted for numerical prediction of maximum flexural capacity for 3D interior RC joints. Results are reliably compatible with the test results and observations as well. Comparing the reported values and numerical ones, the EBROG method appears successfully efficient in preventing FRP debonding and providing the maximum expected contribution of FRP in improving the seismic behavior.
Biodegradability and thermostability of renewable waterborne polyurethane synthesized from trihydroxyl poly(L-malic acid)/glycol based polyols and polycaprolactone diol
Authors: Yu Mingxuan , Song Xin , Niu Haibin , Zhou Chao , Liu Li , Wu Guangfeng 
Published: July 2023

Abstract
A biodegradable composite polyester-based waterborne polyurethane (L-AWPU) was synthesized by coupling L-malic acid and polyethylene glycol to obtain poly(L-malic acid ethylene glycol ester, PL-A) and polycaprolactone (PCL) as raw materials. The content of PL-A in polymer system was controlled to prepare L-AWPU with excellent biodegradable and thermostability properties. Compared to traditional PCL-WPU, the introduction of PL-A not only taking naturally degradable bio-based molecular chains into WPU, but also improving the branching degree of polyurethane molecular chains and formed intramolecular crosslinking structures due to the trifunctional hydroxyl group in malic acid. When the content of PL-A reached 20 wt%, the temperature of thermal weight loss (T5%) and maximum weight loss rate (Tmax) of polyurethane coating increased from 237.93°C and 259.91°C to 333.86°C and 343.13°C, respectively. The establishment of intramolecular crosslinking structures and increase in molecular weight improved the thermal stability of polyurethane. The tensile strength and elongation at break of L-AWPU showed no significant decrease compared to PCL-WPU at PL-A content of 20 wt%. The influence of PL-A content in polymer system on biodegradability of L-AWPU was specifically analyzed. The degradation rates of L-AWPU in 0.6% lipase PBS buffer solution and soil for 28 days reached 45.95% and 55.55% at PL-A content of 40 wt%, which were 1.71-fold and 1.52-fold increased compared to PCL-WPU. Containing natural bio-based molecular chains and trihydroxy crosslinked structures, L-AWPU showed excellent thermal stability and biodegradability, making it suitable for environmentally friendly and recyclable coatings.
Nano1D: An accurate Computer Vision model for segmentation and analysis of low-dimensional objects
Authors: Moradpur-Tari Ehsan , Vlassov Sergei , Oras Sven , Ernits Mart , Damerchi Elyad , Kyritsakis Andreas , Zadin Veronika 
Published: July 2023

Abstract
Microscopy images are usually analyzed qualitatively or manually and there is a need for autonomous quantitative analysis of objects. In this paper, we present a physics-based computational model for accurate segmentation and geometrical analysis of one-dimensional irregular and deformable objects from microscopy images. This model, named Nano1D, has four steps of preprocessing, segmentation, separating overlapped objects and geometrical measurements. The model is tested on Ag nanowires, and successfully segments and analyzes their geometrical characteristics including length, thickness, perimeter and distributions. The function of the algorithm is not undermined by the size, number, density, orientation and overlapping of objects in images. The main strength of the model is shown to be its ability to segment and analyze overlapping objects successfully with more than 99% accuracy, while current machine learning and computational models suffer from inaccuracy and inability to segment overlapping objects. Nano1D can analyze 1D nanoparticles including nanowires, nanotubes, nanorods in addition to other 1D features of microstructures like microcracks, dislocations etc.
The Cauchy Problem of the Three Dimensional Viscoelastic Flows with Initial Data Near Equilibrium
Authors: Fu Shengbin , Huang Wenting , Tian Fangfang 
Published: July 2023

Abstract
This paper explores the Cauchy problem of the three dimensional (3D) compressible viscoelastic fluids system of Oldroyd–B type. More precisely, by utilizing the temporal weighted energy method, we establish the global existence of strong solutions near equilibrium in the H3 (R3) space to this system, which is different from [Hu–Wu, SIAM J. Math. Anal., 45, 2013, 2815– 2833]. Besides, the main result in this paper provides the temporal decay rates of the solution.
Cannulated screw fixation assisted by a self-designed guide for the treatment of Garden stage I-III femoral neck fracture: A retrospective case-series study Running title: Self-designed guide for K-wire insertion
Authors: Li Jianwen , Wang Cheng , Wang Xiao , Chen Cheng , Jiang Jiantao 
Published: July 2023

Abstract
Purpose: To explore the effects of a self-designed Kirschner wire guiding and adjustment apparatus for internal fixation with cannulated screws for the treatment of femoral neck fracture.
Methods: We retrospectively reviewed the patients with femoral neck fracture (Garden I-III) treated at our hospital between 01/2018 and 3/2022. The cases were included in the present study according to the inclusion and exclusion criteria. The operation time, The times of provisional Kirschner wires(K-wires) insertion, times of fluoroscopy , estimated blood loss in operation, Harris score, and VAS score of the hip joint were recorded and analyzed.
Results: Forty patients were included (13 females and 17 males, (median, 58 years of age), with 20 patients in the unguided and 20 in the guided groups, respectively. Their body mass index (BMI) was 23.03±1.86 kg/m2. Compared with the unguided group, the guided group showed shorter operation time (median 0.20 vs. 0.43 h, P<0.001), less blood loss (median, 2 vs. 20 mL, P<0.001), less Insertion times of Kirschner wire (median, 7 vs. 15, P<0.001) and fluoroscopy times (median 12 vs. 20, P<0.001). The VAS score (median, 1vs. 1, P=0.359)and Harris score (92.75±4.83 vs. 89.75±5.20, P=0.066).shows no significant difference between groups at the final follow-up.
Conclusions: Using theself-designed Kirschner wire guiding and adjustment apparatus during fixation of femoral neck fracture with cannulated screws reduces the operation time, blood loss, insertion times of Kirschner wire and fluoroscopy. This might promote the recovery of patients. Those results should be confirmed in further studies.
Levels of Evidence: Level IV