bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemical immunosensor of E. coli O157:H7 in food based on PPY-PANI and quantum dot microsphere conjugates
Authors: Qian JieKe , Lu Haixia , Zheng Shuangzhi , Lu Donghao , Zhu Junli 
Published: August 2023

Abstract
In this study, a sandwich immunosensor based on the conjugate of gold nanoparticles and quantum dot microspheres on polypyrrole/polyaniline (PPY-PANI) composite membrane was developed for the detection of Escherichia coli O157:H7 (E. coli O157:H7). Quantum dot microsphere conjugates (QBC), as immunoprobes throughout electrochemical detection, exhibited signal amplifications. Each stage of the immunosensor preparation was characterized by cyclic voltammetry, and electrochemical impedance spectroscopy. The results showed that the optimal reaction conditions of antigen and secondary antibody were 37 ℃ and 60 min respectively, and the concentration of secondary antibody was 0.01 mg·mL− 1. When E. coli O157:H7 was performed in the immunosensor of PPY-PANI-Ab2, E. coli O157:H7 in the concentration range of 4.73×102-4.73×109 CFU·mL− 1, exhibited a linear relationship between the change of current (ΔIpa) and the logarithm of bacterial concentration. Compared to 100 CFU·mL− 1 of the limit of detection (LOD) in normal saline solution, the lowest detection limit in 0.1% peptone water was 113 CFU·mL− 1, and the signal-to-noise ratio was 3. Moreover, the recovery rate of spiked milk and shrimp were 80.80-98.36% and 75.20-94.31%, and the relative standard deviation (RSD) was 3.03–6.18% and 1.61–6.94% respectively. Therefore, the immunosensor of with low cost and good stability is expected to be used for rapid screening of E. coli O157:H7 in food.
Clinical outcomes and prognostic factors in the surgical treatment of spinal dural arteriovenous fistulas: a retrospective study of 118 patients
Authors: Peng Youheng , Ren Yanming , Hou Jiguang , Zhang Changwei , He Min , Huang Bowen , Chen Tengyun , Li Jin 
Published: August 2023

Abstract
Background and Objective: Spinal dural arteriovenous fistulas (SDAVFs) are the most common type of spinal vascular malformations (AVMs), constituting approximately 70% of all spinal AVMs. The impact of various clinical and radiologic features on the outcomes in patients with SDAVFs is still controversial. The purpose of the study is to investigate the clinical outcomes and prognostic factors in patients with surgically treated SDAVFs in a single center.
Methods: A retrospective review was performed for all patients with SDAVFs from January 2013 to September 2021 who underwent surgery at our institution. Medical records and pre- and postoperative imaging data were analyzed. Neurological function status was evaluated by modified Aminoff-Logue Scale (mALS). Student’s t-test, Wilcoxon rank sum test, χ2 test and logistic regression were used to find significant prognostic factors. P values <0.05 were considered significant.
Results: One hundred and eighteen patients were ultimately included in the study. By comparing preoperative and postoperative mALS, 69 (58.5%) patients experienced improvement, and 49 (41.5%) patients showed no improvement (worse or unchanged) Wilcoxon rank sum test showed that there was a difference between the improvement group and the no improvement group in preoperative mALS Micturition score and preoperative mALS Defecation score. The logistic regression revealed that preoperative mALS Gait score was associated with clinical improvement after surgery in patients with SDAVFs.
Conclusions: Surgical treatment of SDAVFs is a safe and effective procedure and can lead to symptom improvement or stabilization in most patients. Preoperative neurological function status was the only factor associated with clinical prognosis.
The causal relationship between antisocial behavior and resting-state networks: a two- sample Mendelian randomization study
Authors: Zhao Yixin , Guo Weilong , Zhou Jiansong , Wang Xiaoping 
Published: August 2023

Abstract
Background
A growing body of evidence shows that antisocial behavior is associated with resting-state network alterations. However, the causality of this association is not clear. Therefore, we used Mendelian randomization (MR) to examine the causal relationship between antisocial behavior (ASB) and resting-state networks (RSNs).
Methods
Genome-wide association studies summary data of ASB in 85359 participants from the Complex Trait Genetics lab were used to identify their general risk preference. Data from 27744 subjects from the Complex Trait Genetics lab were used to identify the functional connectivity within resting-state networks (RSN-FC). Data from 27397 subjects from the Complex Trait Genetics lab were used to identify the structural connectivity within resting-state networks (RSN-SC). The weighted median, the inverse variance weighted method, and the Mendelian randomization-Egger methods were used for the MR analysis to estimate the causal effect and examine the directional pleiotropy.
Results
GWAS summary data were respectively from three combined samples, which contained 85359, 27744 and 27397 adult participants of European ancestry. MR evidence suggested no significant causal relationship between ASB and RSNs.
Conclusion
Numerous studies have found a close correlation between ASB and RSNs. However, the present MR analysis did not find a direct causal relationship between ASB and RSNs, suggesting that there might be some mediating influencing factors or complex indirect pathways or circuits between ASB and RSNs.
Assessment of Preoperative Malignancy Risk in Pancreatic Cystic Neoplasms through Basic Clinical and Laboratory Parameters
Authors: Martlı Hüseyin Fahri , Acehan Muhammed Fatih , Er Sadettin , Şimşek Ahmet , Şahingöz Eda , Sürel Aziz Ahmet , Tez Mesut 
Published: August 2023

Abstract
Background
The incidence of pancreatic cystic neoplasms (PCN) is increasing over time. However, differentiating between benign and malignant cysts remains challenging. However, identifying between benign and malignant cysts remains difficult. Despite suggestions from many organisations and research groups calling for endosonography and cytology, postoperative pathology is frequently used to make the final diagnosis. This study aimed to investigate the malignancy probability of PCNs using simple clinical and biochemical parameters.
Method
A retrospective analysis was conducted on 70 patients who underwent surgery for pancreatic cystic neoplasms. Based on postoperative pathology, patients were divided into two groups: Group A, which included those with malignancy or high-grade dysplasia, and Group B, which included those with benign or low-grade dysplasia. A comparison was made between pathology results and clinical and laboratory data.
Results
Out of the 70 patients, 42 (60%) were in Group A, and 28 (40%) were in Group B. The mean age was 59.59, and 60% of the patients were male. Univariant analysis (OR 95% CI) revealed that age was 1.095 (1.042–1.150) (p < 0.001), diabetes mellitus was 4.889 (1.710-13.977) (p = 0.003), red cell distribution width (RDW) was 2.240 (1.485–3.380) (p < 0.001), and albumin was 0.914 (0.844–0.989) (p = 0.026). However, in the multivariate analysis, age and RDW were identified as independent variables (OR 95% CI) with values of 1.067 (1.014–1.122) (p = 0.012) and 1.784 (1.172–2.715) (p = 0.007), respectively.
Conclusion
The differentiation between benign and malignant PCN can only be achieved through advanced and specialized investigations. Postoperative pathology is still the most precise means of diagnosis. Age and RDW were shown to be useful in identifying individuals who may benefit from endosonography and cytology, as well as those who needed surgical intervention.
Knockdown of Long Non-coding RNA TUG1 Suppresses pyroptosis in High Glucose-Stimulated BV-2 Cells
Authors: Pan Youzhuo , Lu Xun , Guo Wenhui , Lei Haoyue , Zhang Qi 
Published: August 2023

Abstract
Neuroinflammation is a mechanism by which obesity leads to cognitive impairment. LncRNA-TUG1 can increase neuroinflammation by regulating microglial cells activation, but its mechanism is unclear. The purpose of this study was to investigate the potential functional role of long non-coding RNA TUG1 in high glucose -stimulated BV-2 microglia cells. Mouse microglia cell line BV-2 cells were cultured in vitro and TUG1 shRNA was used to knock down its level. After microglial cells were exposed to high glucose 24 h, TUG1 level, as well as some pyroptosis-associated proteins and releasing inflammatory cytokines were detected by quantitative real-time PCR (qRT-PCR), western blot. The activation of gasdermin D (GSDMD) were detected by immunofluorescence staining, qRT-PCR, and western blot. Our results showed that compared with those in the 5.5 mmol/L glucose control (NC) group, expression of TUG1, NLRP3, caspase1, GSDMD, IL-18 and IL-1β in the 25mmol/L glucose (HG) group of cultured BV-2 cells were significantly increased. the expression of TUG1 was up-regulated in microgolial cells 24 h after high glucose treatment. However, the lncRNA-TUG1 knocked down could significantly reduce the expression of NLRP3, caspase-1 and GSDMD protein and mRNA level in BV-2 cells. Besides, immunofluorescence results showed the lncRNA-TUG1 knocked down stained positively for GSDMD. LncRNA-TUG1 knocked could improve microglial pyroptosis and production of inflammatory cytokines after high glucose insult.
Relationship between Topological efficiency of White Matter Structural Connectome and Plasma Biomarkers across Alzheimer's Disease Continuum
Authors: Zhang Mingkai , Chen Haojie , Huang Weijie , Guo Tengfei , Ma Guolin , Han Ying , Shu Ni 
Published: August 2023

Abstract
Background
Plasma biomarkers and brain network topology have shown potential in early diagnosing Alzheimer's disease (AD). We sought to explore relationships among plasma biomarkers, white matter structural network efficiency, and cognitive performance along the AD continuum.
Materials and Methods
This retrospective study evaluated participants from the Sino Longitudinal Study of Cognitive Decline (SILCODE) cohort between September 2009 and October 2022, with available blood samples or 3.0-T MRI brain scans. Plasma biomarker levels were measured using the Single Molecule Array platform, including β-amyloid (Aβ), phosphorylated tau181 (p-tau181), glial fibrillary acidic protein (GFAP), and Neurofilament light chain (NfL). The topological structure of brain white matter was assessed using network efficiency. Trend analyses and mediation analyses were conducted to investigate the relationships among plasma markers, network efficiency, and cognitive performance across the AD continuum.
Results
498 participants along the AD continuum (mean age, 67.7 years ± 6.7 [SD]; 172 women) were included: 192 with subjective cognitive decline (SCD), 58 with mild cognitive impairment (MCI), 25 with AD, and 223 controls. The study involved 287 blood samples and 395 brain scans. GFAP was the most sensitive marker (t = 11.164, p = 3.59×10− 24), followed by NfL (t = 6.542, p = 2.9×10− 10), p-tau181 (t = 8.452, p = 1.61×10− 15) and Aβ42/Aβ40 (t=-3.257, p = 1.27×10− 3). Local efficiency decreased in the frontal, temporal cortex, and striatum. The principal component of local efficiency within these regions correlated with GFAP (r = − 0.61, p = 6.3×10− 7), NfL (r = − 0.57, p = 6.4×10− 6), and p-tau181 (r = − 0.48, p = 2.0×10− 4). Network efficiency mediated the relationship between cognition and GFAP (β=-0.224, p = .0196) or NfL (β=-0.346, p = .0188).
Conclusions
Network efficiency mediates the association between plasma biomarkers (especially GFAP and NfL) and cognitive performance along the AD continuum. These findings contribute to understanding AD pathogenesis and may have implications for early diagnosis and intervention.
The Magnitude of Trachoma Cases and Associated Factors Among Ophthalmic Clients Attending Menelik II Comprehensive Specialized Hospital, Addis Ababa, Ethiopia
Authors: Legass Getacher , Mideksa Samson , Oumer Abdu , Abegaz Kedir 
Published: August 2023

Abstract
Back ground: Trachoma is the commonest infectious cause of blindness in our globe. It sustained to be endemic in many developing countries like Ethiopia. However, there is no study done in Menelik II comprehensive specialized hospital about the magnitude and associated factors of trachoma. Hence, the current study was to assess the magnitude and risk factors of trachoma infection in Central Ethiopia.
Methods:A hospital based cross-sectional study was employed on 607 ophthalmic clients were selected by consecutive sampling technique. A pretested and structured questionnaire was applied to collect a range of a data on socio demographic, facility and service related and environmental factors. The outcome variable was measured by using frequency, cross-tabulation and percent. Multivariable binary logistic regression model was fitted using stepwise backward regression. We reported adjusted odd ratios with 95% confidence interval and statistical significance was declared at p-value below 0.05.
Result: A total of 607 ophthalmic clients were included in this study. This result revealed that 8.4 % of ophthalmic clients have Trachomatous Trichiasis (95% CI: 6.1%-10.6%). Additionally, TrachomatousScar (2.5%), Trachomatous Follicle (1.8%), and Corneal Opacity (6.1%) were also found in Menelik II comprehensive specialized hospital. Those who were absent of kitchen (AOR= 0.147, 95% CI 0.05–0.40); a present of trash with in the compound (AOR =1.86, 95% CI 1.2-2.8) flies on child face (AOR =3.44, 95% CI 1.3–8.54) and clients without latrine utilization (AOR= 1.78, 95% CI 1.1-2.8) and daily laborers (AOR= 0.47, 95% CI 0.3-0.7) were more likely develop trachoma.
Conclusions:The magnitude of trachoma in ophthalmic clients in the study area was found to be low according to the WHO threshold yet, it is public health threat. Absent of kitchen; poor environmental and personal sanitary conditions were relevant risk factors which could be further targeted in the fight against trachoma.
N-Redundant UDP - a fault tolerance scheme for real-time notification
Authors: Syam Anupom 
Published: August 2023

Abstract
UDP is a very efficient connectionless transmission model. It’s a well-suited protocol to use in real-time applications. As we know the problem with UDP is unreliability and lack of ordering. Many schemes and protocols were suggested or developed on top of UDP to make it reliable and ordered. But in all those cases we sacrifice latency to guarantee reliability. There are practical use cases where UDP is used for inter-system event notification (as an example, real-time event notification within a distributed system) where each event is transferred as a tiny datagram. In such cases increasing reliability without hampering the latency is desired. In this paper, we propose a scheme named N-redundant UDP that is suitable for transmitting small messages with increased Quality of Service than plain UDP but without adding any latency burden.
Efficacy and safety comparison of infrared laser moxibustion and traditional moxibustion in knee osteoarthritis: Study protocol for a Zelen- design randomized controlled non-inferiority clinical trial
Authors: Wang Zhong-yu , Chen Fang-fang , Guo Shi-qi , Xue Ying , Zhang Jia-chen , He Jing , Shi Yu-qing , OuYang Xia-li , Liu Ping , Zhang Yang , Shuang Shuang , Chen Yi-xiang , Li Jiang-tao , Zhao Bai-xiao , Han Li 
Published: August 2023

Abstract
Background
Knee osteoarthritis (KOA), which is the most common chronic degenerative joint disease, places substantial burden on public health resources in China. The purpose of this study is to preliminary evaluate whether infrared laser moxibustion (ILM) is non-inferior to traditional moxibustion (TM) in the treatment of KOA.
Methods
In the designed Zelen-design randomized controlled non-inferiority clinical trial, a total of 74 patients with KOA will be randomly allocated to one of two interventions: ILM treatment or TM treatment. All participants will receive a 6-week treatment and be followed-up for 4 weeks. The primary outcome will be the mean change in pain on the numeric rating scale (NRS). The secondary outcomes will be the pain on the NRS from weeks 1 to 5 after the start of treatment and the changes from baseline to endpoints (weeks 6 and 10) in the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), SF-36, knee circumference, and 6-min walking test. In addition, safety assessment will be performed throughout the trial.
Discussion
The results of our study will help determine whether a 6-week treatment with ILM is non-inferior to TM in patients with KOA, therefore providing evidence for verifying if ILM can become a safer alternative for TM in clinical applications in the future.
Trial registration:
Clinical Trial Registration Platform (ChiCTR2200065264); Pre-results. Registered on 1 November 2022.
Identification of prognostic biomarkers among VGCCs family in human ovarian cancer
Authors: Li Tingting , Gao Wen , Shi Peipei , Yang Lina , Yin Sheng , Ren Aimin , Zhang Jiarong 
Published: August 2023

Abstract
Background
The disease burden of suffering epithelial ovarian cancer (EOC) for women is increasing evidently with high risk of recurrence and poor prognosis. There is critical need to explore novel approaches for efficient treatment. VGCCs, a group of genes that encode a family of voltage-gated calcium channel, have been identified as being involved in the tumor progression of various cancer types. We performed a bioinformatics analysis to identify the expression and prognostic values of different VGCC family in ovarian cancer.
Methods
In the first place, we analyzed the transcript expression of VGCCs in clinical cancer tissue vs normal samples by accessing ONCOMINE and GEPIA microarray database. Subsequently, we analyzed the correlation between the gene expression, genetic alteration, and patient’s prognosis by utilizing the cBioPortal and Kaplan-Meier plotter. Furthermore, we performed immunohistochemical (IHC) in tissue from ovarian cancer patient to validate the results.
Results
Notably, the majority of VGCC family members exhibited low expression in various cancer subtypes, including ovarian cancer. However, Cav2.1 (CACNA1A), encoding the P/Q type calcium channel, exhibited up-regulated expression in ovarian cancer tissue compared to normal tissue. Moreover, Cav2.1 achieved a relative high mutation in ovarian cancer patients, of which the majority was mRNA amplification. Interestingly, both overexpression of Cav2.1 and genetic variation were correlated with poorer PFS and OS. Moreover, our immunohistochemistry results from EOC patients verified that Cav2.1 was statistically related to poorer prognosis.
Conclusion
Collectively, our results hypothesized CACNA1A amplification may contribute to the upregulation of Cav2.1 expression, that were correlated with poor prognosis of EOC. Cav2.1 may serve as therapeutic targets and potential oncogene of ovarian cancer.
Seismic hazard indicators in Japan based on seismic noise properties
Authors: Lyubushin Alexey 
Published: August 2023

AbstractThe seismic noise recorded at the network of stations in Japan for more than 26 years (1997 - March 2023) is being investigated. The article is a continuation of the number of works in which the analysis was performed for 1997 - March 2021. New data for 2 years of follow-up observations, combined with a new approach to their analysis, provided additional opportunities to test a number of hypotheses regarding the presence of features in the properties of seismic noise that precede the release of seismic energy. The analysis is based on the use of the wavelet-based Donoho-Johnstone index, as a property of noise waveforms, the use of an auxiliary network of reference points, the estimation of spatial correlations of noise properties, and the relationship of the seismic noise response to the irregular rotation of the Earth with the flow of seismic events in the vicinity of the Japanese Islands by using a parametric model of interacting point processes.
Comparison of contamination and infection of CoNS bacteremia in hospitalized children’s blood cultures
Authors: Okay Songul , Gülhan Belgin , Yüksek Saliha Kanık , Parlakay Aslınur Özkaya , Bayhan Gülsüm İclal 
Published: August 2023

Abstract
Purpose: We have aimed to evaluate the clinical significance of infections caused by coagulase- negative staphylococci in pediatric patients.

Methods: Study included 511 patients aged 1 month-18 years with growth of coagulase-negative staphylococci (CoNS) in their blood cultures obtained between October 2020-October 2021. The presence of blood stream infection (BSI), catheter- related bloodstream infection (CRBSI) or contamination was investigated.

Results: The median age of the patients was 2.2 years (min:1 month, max:18 years). The study population consisted of 57.9% male, and 42.1% female patients. Contamination was detected in 82.2%, bloodstream infection (BSI) in 10.8% and catheter-related bloodstream infection (CRBSI) in 7% of patients, while S. epidermidis was identified in 58.5%, S. hominis in 40.3% and S. haemolyticus in 13.9% of them. The rate of methicillin-resistant CoNS was found to be 36.8%. Fever, indwelling catheter use, total parenteral nutrition were significantly more common in the CRBSI/BSI group than in the contamination group (p=0.017; p<0.001; p=0.001, respectively). The growth of S. haemolyticus was found to be mostly associated with CRBSI/BSI, and S. epidermidis was considered more often as an contaminant agent (p<0.001; p=0,022, respectively) The duration of hospital stay was longer in the CRBSI/BSI group, and antibiotherapy changed more frequently in the CRBSI/BSI group (p<0.001, p<0.005, respectively).
Conclusions: Accompanying fever, using an intravascular device, and TPN may be warning signs for CRBSI or BSI. The most effective way to reduce unnecessary use of antibiotics, laboratory workload and medical expenditures is to prevent contamination by performing correct blood collection procedures.
5-HT_FAsTR: A versatile, label-free, microplate assay to quantify serotonin transport and release using the genetically encoded fluorescent biosensor iSeroSnFR
Authors: Bukowski Lina , Strøm Markus , Andersen Jens , Maesen Jannick , Tian Lin , Sinning Steffen 
Published: August 2023

Abstract
The neurotransmitter serotonin is important in mood and depression, acts as a vasoconstrictor in blood vessels and is the main neurotransmitter of the gut. In neurotransmission, the released serotonin is taken up by serotonin transporters, which are principal targets of antidepressants and the psychostimulant, ecstasy. Studies of serotonin transporters have relied almost exclusively on the use of radiolabeled serotonin in heterogenous end-point assays. Here we present the establishment and validation of the Serotonin (5-HT) Fluorescence Assay for Transport and Release (5-HT_FAsTR) for functional and pharmacological studies of serotonin transport and release. We demonstrate the applicability of the method for the study of a neuronal, high-affinity, low-capacity serotonin transporter (SERT) as well as an extraneuronal low-affinity, high-capacity organic cation transporter (OCT) and mutants thereof. 5HT_FAsTR is an accessible, versatile and reliable semi-homogenous assay format that only relies on a fluorescence plate reader for repeated, real-time measurements of serotonin influx and efflux.