bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of Episode of Hemodynamic Instability Using an Electrocardiogram Based Analytic: A Retrospective Cohort Study
Authors: Benson Bryce , Belle Ashwin , Lee Sooin , Bassin Benjamin S. , Medlin Richard P. , Sjoding Michael W. , Ward Kevin R. 
Published: June 2023

Abstract
Background
Predicting the onset of hemodynamic instability before it occurs remains a sought-after goal in acute and critical care medicine. Technologies that allow for this may assist clinicians in preventing episodes of hemodynamic instability (EHI). We tested a novel noninvasive technology, the Analytic for Hemodynamic Instability-Predictive Indicator (AHI-PI), which analyzes a single lead of electrocardiogram (ECG) and extracts heart rate variability and morphologic waveform features to predict an EHI prior to its occurrence.
Methods
Retrospective cohort study at a quaternary care academic health system using data from hospitalized adult patients between August 2019 and April 2020 undergoing continuous ECG monitoring with intermittent noninvasive blood pressure (NIBP) or with continuous intra-arterial pressure (IAP) monitoring.
Results
AHI-PI’s low and high-risk indications were compared with the presence of EHI in the future as indicated by vital signs (heart rate > 100 beats/min with a systolic blood pressure < 90 mmHg or a mean arterial blood pressure of < 70 mmHg). 4,633 patients were analyzed (3,961 undergoing NIBP monitoring, 672 with continuous IAP monitoring). 692 patients had an EHI (380 undergoing NIBP, 312 undergoing IAP). For IAP patients, the sensitivity and specificity of AHI-PI to predict EHI was 89.7% and 78.3% with a positive and negative predictive value of 33.7% and 98.4% respectively. For NIBP patients, AHI-PI had a sensitivity and specificity of 86.3% and 80.5% with a positive and negative predictive value of 11.7% and 99.5% respectively. Both groups performed with an AUC of 0.87. AHI-PI predicted EHI in both groups with a median lead time of 1.1 hours (average lead time of 3.7 hours for IAP group, 2.9 hours for NIBP group).
Conclusions
AHI-PI predicted EHIs with high sensitivity and specificity and within clinically significant time windows that may allow for intervention. Performance was similar in patients undergoing NIBP and IAP monitoring.
TGFβ signaling links early-life endocrine-disrupting chemicals exposure to suppression of nucleotide excision repair in rat myometrial stem cells
Authors: Bariani Maria Victoria , Cui Yan-Hong , Ali Mohamed , Bai Tao , Grimm Sandra L. , Coarfa Cristian , Walker Cheryl L. , He Yu-Ying , Yang Qiwei , Al-Hendy Ayman 
Published: June 2023

Abstract
Environmental exposure to endocrine-disrupting chemicals (EDCs) is linked to the development of uterine fibroids (UFs) in women. UFs, non-cancerous tumors, are thought to originate from abnormal myometrial stem cells (MMSCs). Defective DNA repair capacity may contribute to the emergence of mutations that promote tumor growth. The multifunctional cytokine TGFβ1 is associated with UF progression and DNA damage repair pathways. To investigate the impact of EDC exposure on TGFβ1 and nucleotide excision repair (NER) pathways, we isolated MMSCs from 5-months old Eker rats exposed neonatally to Diethylstilbestrol (DES), an EDC, or to vehicle (VEH). EDC-MMSCs exhibited overactivated TGFβ1 signaling and reduced mRNA and protein levels of NER pathway components compared to VEH-MMSCs. EDC-MMSCs also demonstrated impaired NER capacity. Exposing VEH-MMSCs to TGFβ1 decreased NER capacity while inhibiting TGFβ signaling in EDC-MMSCs restored it. RNA-seq analysis and further validation revealed decreased expression of Uvrag, a tumor suppressor gene involved in DNA damage recognition, in VEH-MMSCs treated with TGFβ1, but increased expression in EDC-MMSCs after TGFβ signaling inhibition. Overall, we demonstrated that the overactivation of the TGFβ pathway links early-life exposure to EDCs with impaired NER capacity, which would lead to increased genetic instability, arise of mutations, and fibroid tumorigenesis. We demonstrated that the overactivation of the TGFβ pathway links early-life exposure to EDCs with impaired NER capacity, which would lead to increased fibroid incidence.
A multi-functional hypoxia/esterase dual stimulus responsive and hyaluronic acid-based nanomicelle for targeting delivery of chloroethylnitrosouea
Authors: Li Duo , Ren Ting , Ge Yunxuan , Wang Xiaoli , Sun Guohui , Zhang Na , Zhao Lijiao , Zhong Rugang 
Published: June 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
“Wrinkles will only go where the smiles have been”: A path analysis of the associations between happiness and health, sleep, physical activities for older people in Abu Dhabi
Authors: Badri Masood , Alkhaili Mugheer , Aldhaheri Hamad , Yang Guang , Yaaqeib Saad , Albahar Muna , Alrashdi Asma 
Published: June 2023

Abstract
Background
The study aimed to identify the associations of happiness and factors related to physical and mental health, leisure, and sports activities amongst older adults in Abu Dhabi. The sample comprised 1,004 participants in the third Abu Dhabi Quality of Life survey administered in 2019-2020.
Methods
The analysis used path analysis to develop a model incorporating the specified variables. The path model highlighted all direct and indirect associations between the variables. We also used variance analysis to test the differences in gender, marital status, and education attainment with happiness.
Results
Results show that sleep quality is most associated with happiness and subjective health. In addition, sleeping hours did not show any association with subjective health; but were associated with happiness. The result also confirms that mental health is negatively associated with happiness and subjective health. How often an elderly gets involved in sport and activities for at least 30 minutes significantly affects subjective health and happiness.
Conclusions
Happiness of older adults is best understood when we look at both direct and indirect effects using a path model. Their happiness is significantly associated with their subjective health, mental health, participation in sport and activities and sleep quality, Implications of the study were highlighted, along with future research directions.
Metabolic engineering Escherichia coli for high-level production of pseudouridine
Authors: Zhou Min , Tang Ruyu , Wei Liyuan , Wang Jidong , Qi Huan 
Published: June 2023

Abstract
Background
Pseudouridine-incorporated mRNA vaccines can enhance protein expression and reduce immunogenicity, leading to a large demand for pseudouridine to be used in the production of mRNA drugs. The production of pseudouridine by chemical or semienzymatic synthesis is limited by low yields and high costs, and the production of pseudouridine via the fermentation of genetically engineered strains is an attractive prospect.
Results
In the present study, a cell factory for the efficient synthesis of pseudouridine was established. First, the pyrimidine precursor biosynthesis pathway in Escherichia coli was systematically modified to increase metabolite flux to pseudouridine biosynthesis. Next, two crucial genes (pseudouridine 5’-phosphate glycosidase gene (psuG) and phosphatase gene) were screened. An artificial pseudouridine biosynthesis pathway with high efficiency was constructed. The accumulation of pseudouridine was further increased by the deletion of pseudouridine catabolism-related genes. Ultimately, the pseudouridine titre in a 5-L bioreactor reached 7.5 g/L.
Conclusion
In this study, a cell factory producing a high yield of pseudouridine was successfully constructed and showed the potential for use in industrial production.
Analysis of death characteristics of 1266 cancer patients
Authors: Wei Xing , Tan HongXia , Tu YueHe , Wang ShaoWen , Qu LiXin , Li Xiang 
Published: June 2023

Abstract
Purpose
To explore the influence of different treatment schemes on the survival time of dying cancer patients and the characteristics of patients' death time.
Method
Group A: patients went to the Intensive care unit (ICU) for further treatment and were given mechanical assistance and other rescue measures; Group B: patients accepted drug rescue treatment in the general ward, and CPR was given in case of cardiac arrest. Group C: patients accepted only drug rescue treatment; Group D: No rescue treatment. We calculated the survival time of patients in different groups. We used circular statistics to analyze whether there was a significant trend in the month of death.
Results
The total survival time of group A was 138.0h. (95% confidence interval [CI], 109.1 to 166.8) versus group B 54.5h (95% [CI], 42.8 to 66.3) versus group C 60.0h (95% [CI], 51.7 to 68.3) versus group D 60.4 (95% [CI], 53.9 to 66.8). There was significant statistical significance between groups A vs. groups B, C, and D (P < .05), and there was no statistical difference between groups B, C, and D (P > .05). The deaths occurred in October of each year.
Conclusions
Medicated rescue therapy and CPR are ineffective for patients with endangered cancer, ICU treatment can prolong the survival time of dying patients. More cancer patients die in October.
Electrical analogue of one-dimensional and quasi-one-dimensional Aubry-André-Harper lattices
Authors: Maiti Santanu , Ganguly Sudin 
Published: June 2023

Abstract
The present work discusses the possibility to realize correlated disorder in electrical circuits and studies the localization phenomena in terms of two-port impedance. The correlated disorder is incorporated using the Aubry-André-Harper (AAH) model. One-dimensional and quasi-one-dimensional AAH structures are explored and directly mapped with their tight-binding analogues. Transitions from the high-conducting phase to the low-conducting one are observed for the circuits.
Study of the reverse transition in pipe flow
Authors: Kanda Takeshi , Ykoo Hikaru , Yamamoto Mizuki , Matsumoto Takumi , Yamada Takahiro 
Published: June 2023

Abstract
In the reverse transition in pipe flow, turbulent flow changes to less disturbed laminar flow. The entropy of the flow appears to decrease. This study examined the reverse transition experimentally and theoretically using entropy change and momentum balance models, not in terms of disturbance in the flow. The reverse transition was accomplished by decreasing the Reynolds number. The transitions approximately correlated with local Reynolds numbers. The initial Reynolds number of the transition became larger, and the pressure at low Reynolds numbers was greater than in ordinary pipe flow. These behaviours were caused by turbulent flow in the pipe undergoing a reverse transition. We showed that the entropy did not decrease in the reverse transition by including the entropy due to friction in the development region.
Identification of Potential Therapeutic Target SPP1 and Related RNA Regulatory Pathway in Keloid Based on Bioinformatics Analysis
Authors: Xie Ruxin , Zhang Shiwei , Yun Jiao , Zhong Ai , Wu Junliang , Cen Ying , Chen Junjie 
Published: June 2023

Abstract
Background
To explore the complex mechanisms of keloid, new approaches have been developed by different strategies. However, conventional treatment did not significantly reduce the recurrence rate. This study aimed to identify new biomarkers and mechanisms for keloid progression through bioinformatics analyses.
Methods
Microarray datasets for keloid were downloaded from the GEO database. Differentially expressed gene were identified by R software. Multiple bioinformatics tools were used to identify hub genes, and reverse predict upstream miRNAs and lncRNA molecules of target hub genes. Finally, the total RNA-sequencing technique and miRNA microarray were combined to validate the identified genes.
Results
Thirty-one DEGs were screened out and the upregulated hub gene SPP1 was finally identified, which was consistent with our RNA-sequencing analysis results and validation dataset. In addition, a ceRNA network of mRNA (SPP1)-miRNA (miR-181a-5p)-lncRNA (NEAT1, MALAT1, LINC00667, NORAD, XIST and MIR4458HG) was identified. The results of our miRNA microarray showed that miR-181a-5p was upregulated in keloid, also we found that the lncRNA NEAT1 could affect keloid progression by retrieving the relevant literature.
Conclusions
We speculate that SPP1 is a potential candidate biomarker and therapeutic target for patients with keloid, and NEAT1/miR-181a-5p/SPP1 might be the RNA regulatory pathway that regulates keloid formation.
Graph-based approach for enumerating the Nash equilibria of a two-player extensive-form game
Authors: Zappalà Paolo , Benhamiche Amal , Chardy Matthieu , De Pellegrini Francesco , Figueiredo Rosa 
Published: June 2023

Abstract
Enumerating the Nash equilibria of a game is a known problem of game theory. We take into account two-player extensive-form games with perfect information and perfect recall. Based on the notion of compatibility of strategies, a new graph representation permits to devise a new lightweight algorithm to enumerate all of its Nash equilibria. It is the first of its kind not to use brute force. The method provides also intermediate results, like upper (lower) bounds to the value of the utility of Nash equilibria or the enumeration of Nash equilibria that fulfill any given constraints (possibly, unconstrained) on their values. We compare our method to the only existing formulation providing an upper bound to the value of the utility of any Nash equilibrium. The experiments show that our method is faster by some orders of magnitude. We also test the method to enumerate the Nash equilibria on a new library, that we introduce as benchmark for representing all structures and properties of extensive-form games.
Extraction of new exopolysaccharide from K. oxytoca J7 and its possible application in biosorption of Ni 2+ ions from contaminated water
Authors: Ljubic Verica , Perendija Jovana , Cvetkovic Slobodan , Rogan Jelena , Trivunac Katarina , Stojanovic Marijana , Popovic Mina 
Published: June 2023

Abstract
Nowadays, exopolysaccharides (EPS) produced from bacterial cells are manufactured for their use in different industries in the world, mainly in the food, pharmaceutical, and wastewater industries. The characteristics of EPS, such as being biodegradable, safe, high adsorption capacity, and reusable, make them significant and potential applications in the purification of contaminated water of heavy metals. In this study, the possible application in biosorption Ni2+ ions from contaminated water was assessed using this exopolysaccharide as a biosorbent. The new exopolysaccharide from the bacterial strain K. oxytoca J7 was extracted, isolated, and characterized using SEM, FTIR, XRD, TGA/DTG, and MALDI-TOF MS analysis. Likewise, the cytotoxic activity was performed for EPS from K. oxytoca J7 strain. The aim of this study was to investigate the possible application of non-toxic exopolysaccharide in the purification of contaminated water by removing Ni2+ ions. The results obtained from the biosorption study showed that the Langmuir model is well suited to describe the adsorption process of Ni2+ ions by EPS from K. oxytoca J7, with a maximum adsorption capacity of 269.97 mg g–1. The importance of this study is the possible use of natural nontoxic exopolysaccharide extracted from the pathogen microorganism, K. oxytoca J7, for the removal of Ni2+ ions from the contaminated water.
Colliding Plane Gravitational Waves of Unequal Strength
Authors: Abbasi Kamran Qadir , Qadir Asghar 
Published: June 2023

Abstract
Khan and Penrose, and separately Szekeres, presented solutions of colliding impulsive and sandwich plane gravitational waves, respectively. They only considered equal strength waves, which would impart no momentum to test particles in their path on the plane of collision at the time. We had earlier probed both spacetimes using the pseudo-Newtonian (ψN) formalism. In neither case was it really clear what parameter represented the “strength” of the wave. In this paper we identify the required parameter and extend the colliding sandwich plane gravitational wave solution to unequal strengths. Since the impulsive plane waves of Khan and Penrose correspond to a limit of the sandwich waves with the pulse duration tending to zero, we have used the procedure to obtain the generalization of the Khan-Penrose solution to unequal strengths. The resulting spacetimes are exact solutions of vacuum field equations.
Associations of serum cortisol and inflammatory marker features with depression and anxiety in young women with gynecologic cancer
Authors: Qin Ning , Yi Shuijing , Dai Chanyuan , Liu Xiangyu , Duan Yinglong , Zhou Yi , Wan Ziyu , Kang Yue , Zhou Xing , Xie Jianfei , Cheng Andy 
Published: June 2023

Abstract
Purpose
Serum cortisol and inflammatory markers may play a role in depression and anxiety, but little is known about whether various features of serum cortisol and inflammatory markers have different associations with depression and anxiety. This study examines the associations of serum cortisol and inflammatory marker features with depression and anxiety in young women with gynecologic cancer.
Methods
Sixty-four young women with gynecologic cancer, aged 15–39 years, were recruited in a tertiary general hospital in China from May to December 2021. The Hospital Anxiety and Depression Scale was used to evaluate depression and anxiety. Blood samples were taken at 8am, 4pm and 10pm on the same day to examine the various features (average, variability and diurnal patterns) of serum cortisol and inflammatory markers, namely C-reactive protein (CRP), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α).
Results
Young women with gynecologic cancer who reported depression/anxiety had significantly higher average levels of serum cortisol, IL-6 and TNF-α than those who did not. The dysregulations in the diurnal patterns of serum cortisol and IL-6 were associated with depression and anxiety. Serum cortisol levels were significantly higher in the depression/anxiety group at 10pm. Depression and anxiety were associated with elevated levels of IL-6 and TNF-α at each time point.
Conclusion
This study revealed various associations of serum cortisol and inflammatory marker features with depression and anxiety in young women with gynecologic cancer. Further research is needed to understand the role of serum cortisol and inflammatory marker features in the progression of depression and anxiety.
Perceived Social Benefits and Drawbacks of Sea Turtle Conservation Efforts in a Globally Important Sea Turtle Rookery
Authors: Vieira Sara , Jiménez Victor , Ferreira-Airaud Betânia , Pina Antunes , Soares Venceslau , Tiwari Manjula , Teodósio Maria A. , Castilho Rita , Nuno Ana 
Published: June 2023

Abstract
Conservation interventions have wide-ranging social impacts - both positive and negative. Yet a limited understanding of how conservation initiatives affect people’s livelihoods often hinders our ability to learn from past efforts and design more effective and equitable conservation measures. This is particularly needed when there is a high degree of overlap between critical habitats and human activities or a high cultural and economic demand for products derived from the conservation target. Here, we explore the social impacts of sea turtle conservation initiatives implemented on São Tomé Island (Gulf of Guinea) as a case study and consider how these might enhance or hinder future efforts. Semi-structured interviews were conducted with key actors involved in the sea turtle trade in December 2014-February 2015 (prior to the implementation of key initiatives) and February-April 2022. Our findings suggest a clear reduction in the scale of the sea turtle trade and the number of main actors involved. However, most of the interviewees previously involved in the trade had experienced economic displacement and several associated social impacts, such as the reduced ability to support family and friends and food insecurity, due to trade restrictions. Financial capital was the main barrierto transitioning to alternative livelihoods, followed by the lack of skills and peer pressure. Finally, this study highlights the importance of considering human dimensions during the planning and implementation phase of conservation actions and the need for more investment focused on the well-being of communities to ensure the long-term survival of endangered species.
Innovative solutions and sensitivity analysis of a fractional complex Ginzburg-Landau equation
Authors: Leta Temesgen Desta , Chen Jingbing , Achab Abdelfattah El 
Published: June 2023

Abstract
A diverse range of traveling wave structures of fractional complex Ginzburg-Landau equation with Kerr law and power-law nonlinearity are obtained by using the bifurcation method. The existence of wave solutions is guaranteed by reporting constraint conditions and with the help of traveling wave transformation the governing model is converted into the planar dynamical system. Every bounded phase orbits are plotted for pertinent parameters. We also extract the nonlinear periodic solutions of the considered problem and outcomes are presented graphically with the help of contemporary software that allows computation of equations within the symbolic format. Furthermore, the quasiperiodic and chaotic behavior and sensitivity of the model is analyzed for different values of parameters after deploying an external periodic force and on some initial value.
MSC Classi cation: 34K20 , 34K18 , 37G10 , 78M25
Quantitative measures of the medial and lateral novel meniscus parameters predict incident radiographic knee osteoarthritis: the Osteoarthritis Initiative
Authors: Bo Kaida , Liu Xin , Ou Jianliang , Zhang Yuanyi , Wang Xu , Yang Shuo , Zhang Wei , Zhang Lelei , Chang Jun 
Published: June 2023

Abstract
Background: To determine whether novel meniscus parameters are predictive of incident radiographic knee osteoarthritis (ROA) over four years in the Osteoarthritis Initiative (OAI) study.
Methods: Participants were extracted from the Osteoarthritis Initiative (OAI) study. 178 knees (from 176 patients ) with incident ROA (Kellgren Lawrence Grade (KLG) 0 or 1 at baseline (BL), developing KLG 2 or greater by year 4) were matched one-to-one by gender, age and radiographic status with 178 control knees (from 178 patients) not developing incident ROA. Novel meniscal parameters were measured on coronal T2-weighted turbo spin echo (TSE) MRI. Conditional logistic regression analyses were used to assess the risk of incident ROAassociated with novel meniscus parameters after adjustment for covariates. Sensitivity analysis was performed for P0 (time to visit when ROA was found on the radiographs) , P1 (1 year prior to P0) and baseline (enrollment period) ROA incidence.
Results: Intra-class and inter-class reliabilities of measurement of novel parameters were excellent (≥0.89). Baseline meniscus measurement parameters included the mean of MLD [mean(MLD)], the mean of RMLD [mean(RMLD)] and the mean of TPW[Mean(TPW)]. Baseline Mean(MLD) and Mean(RMLD) [Mean(MLD), Mean(RMLD)] [Adjusted OR, 95%CI: 1.11(1.07 to 1.16) and 1.13(1.09 to 1.17), respectively] were associated with incident ROA during 4 years, but mean value of tibial plateau width [Mean(TPW)] [Adjusted OR, 95%CI: 0.98(0.94 to 1.02)] was not associated with incident ROA during 4 years. At baseline, Mean (MLD) and Mean (RMLD) were significantly different between case and control groups. While P1 and P0 [Mean(TPW)] were not associated with incident ROA risk, other measures of P1 and P0 were significantly positivelyassociated with incident ROA risk.
Conclusions: The novel method of measuring meniscus novel parameters on MRI is reproducible. Compared with the control group, Mean(MLD) and Mean(RMLD) at baseline, P1 and P0 periods are higher in the case group than in the control group and the measurement differed significantly among the groups. These novel meniscus parameters alterations could be an important imaging biomarker to predict the occurrence of ROA.