bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermophilic bacteria and their thermozymes in composting processes: a review
Authors: Finore Ilaria , Feola Antonio , Russo Ludovica , Cattaneo Andrea , Di Donato Paola , Nicolaus Barbara , Poli Annarita , Romano Ida 
Published: January 2023

AbstractIn this review, the composting process of organic waste is discussed through an in-depth exploring of its thermophilic phase. It starts with the highlight on the thermodynamic evolution, which needs to be assessed when deciding to use reactors for composting, also in the context of energy generation. The composting process is mediated by different types of microorganisms, and the bacteria that play key roles are evaluated. The roles of the generaBacillusandThermusare considered, often described as the main components of the microbiota of compost. Due to their adaptation to the composting processes, they are candidates for technological purposes. Subsequentially, the focus is moved on the thermostable enzymes that can be isolated from them and their succession during the composting processes. Experimental examples of enzyme-related literature are reviewed, for example investigating proteases and ureases, which are found at the beginning of the process. In addition, cellulases, hemicellulases, lignin-modifying enzymes, and esterases have been described for their activities during the thermophilic phase, giving them great potential for biotechnological and industrial applications. Following, the composition of the microbial community is analyzed through the description of approaches of metagenomics. Despite it being a relatively new but fast-growing field within biology, it is intended to be a priority analysis to acquire knowledge on genomes of environmental microorganisms and communities. Finally, a space is dedicated to the description of the composting plant which treats olive oil wastes within the LIFE TIRSAV PLUS project (LIFE05 ENV/IT/00845). Through two plant solutions, being the Dynamic and the Static Composting, it provides a high-quality compost with an effective, flexible and economical process.Graphical Abstract
Deep learning-enhanced nuclear medicine SPECT imaging applied to cardiac studies
Authors: Apostolopoulos Ioannis D. , Papandrianos Nikolaos I. , Feleki Anna , Moustakidis Serafeim , Papageorgiou Elpiniki I. 
Published: January 2023

AbstractDeep learning (DL) has a growing popularity and is a well-established method of artificial intelligence for data processing, especially for images and videos. Its applications in nuclear medicine are broad and include, among others, disease classification, image reconstruction, and image de-noising. Positron emission tomography (PET) and single-photon emission computerized tomography (SPECT) are major image acquisition technologies in nuclear medicine. Though several studies have been conducted to apply DL in many nuclear medicine domains, such as cancer detection and classification, few studies have employed such methods for cardiovascular disease applications. The present paper reviews recent DL approaches focused on cardiac SPECT imaging. Extensive research identified fifty-five related studies, which are discussed. The review distinguishes between major application domains, including cardiovascular disease diagnosis, SPECT attenuation correction, image denoising, full-count image estimation, and image reconstruction. In addition, major findings and dominant techniques employed for the mentioned task are revealed. Current limitations of DL approaches and future research directions are discussed.
Neuropathology of yellow fever autopsy cases
Authors: Frassetto Fernando Pereira , Rosemberg Sergio 
Published: January 2023

Abstract
Background
Yellow fever is a viral hemorrhagic fever caused by yellow fever virus, a mosquito-borne flavivirus. Despite an effective vaccine, major outbreaks continue to occur around the world. Even though it is not a proven neurotropic virus, neurological symptoms in more severe clinical forms are frequent. The understanding of this apparent paradox is still rarely addressed in literature.

Methods
The brains of thirty-eight patients with yellow fever confirmed by RT-PCR, who underwent autopsy, were analyzed morphologically to identify and characterize neuropathological changes. The data were compared with brains collected from individuals without the disease, as a control group. Both cases and controls were subdivided according to the presence or absence of co-concurrent septic shock, to exclude changes of the sepsis associated encephalopathy. To verify possible morphological differences between the yellow fever cases groups, between the control groups, and between the cases and the controls, we applied the statistical tests Fisher's exact test and chi-square, with p values < 0.05 considered statistically significant.

Results
All cases and controls presented, at least focally, neuropathological changes, which included edema, meningeal and parenchymal inflammatory infiltrate and hemorrhages, and perivascular inflammatory infiltrate. We did not find an unequivocal aspect of encephalitis. The only parameter that, after statistical analysis, can be attributed to yellow fever was the perivascular inflammatory infiltrate.

Conclusions
The neuropathological findings are sufficient to justify the multiple clinical neurologic disturbances detected in the YF cases. Since most of the parameters evaluated did not show statistically significant difference between cases and controls, an explanation for most of the neuropathological findings may be the vascular changes, consequent to shock induced endotheliopathy, associated with stimulation of the immune system inherent to systemic infectious processes. The statistical difference obtained in yellow fever cases regarding perivascular infiltrate can be can be explained by the immune activation inherent to the condition.
A case report: extracorporeal membrane oxygenation for multitrauma patient with pneumorrhagia
Authors: Meng Qiang , Ji Changwei , Ma Zhen , Chen Bo wang , Zhang Hao , Li Jun , Cong Lin , Qu Feng , Gao Shuhong 
Published: January 2023

Abstract
Background
Extracorporeal membrane oxygenation (ECMO) can be used as salvage therapy for multitrauma patients with acute respiratory distress syndrome (ARDS) when conventional treatment fails to maintain oxygenation. However, controversy exists between ECMO application and the risk of bleeding due to systemic anticoagulation during the treatment. Non-heparin introduction seems to be a possible solution for this dilemma, owing to technical improvements in the device and management methods of ECMO.

Case presentation
A 58-year-old woman suffered from blunt thoracic, pelvic, and right lower limb fractures due to a falling accident, which resulted in acute respiratory distress syndrome (ARDS). Although the patient received intubation and mechanical ventilation (MV), respiratory failure was not alleviated. Venous-venous (V-V) ECMO was used as a salvage therapy. With the support of V-V ECMO, we safely cleared blood clots in the bronchus and avoided secondary lung injury caused by pressure trauma and oxygen poisoning of the MV. We introduced heparin-free ECMO management as a solution to reduce the risk of bleeding associated with pulmonary contusion and other organ injuries. To prevent thrombosis, we set the blood rate of ECMO to 4.0 L/min, which is much higher than the usual parameter. During ECMO, coagulation factors, such as prothrombin time, activated partial thromboplastin time, and D-dimer, were examined. ECMO was maintained for 5 days without any complications; MV was stopped on the 13th day, extubated on the 24th day, and discharged from ICU on the 28th day.

Conclusion
ECMO with non-heparin could be an optimal treatment for multitrauma patients with ARDS when traditional treatment cannot sustain oxygenation. High blood flow rate could prevent thrombosis through ongoing ECMO therapy without systemic anticoagulation. In addition, monitoring D-dimer value change (Δ D-dimer) may be better than D-dimer value in predicting clot formation in the membrane oxygenator.
Clinician Perspectives on Barriers and Enablers to Implementing an Inpatient Oncology Early Warning System: A Mixed-Methods Study
Authors: Lyons Patrick G. , Chen Vanessa , Sekhar Tejas C. , McEvoy Colleen A. , Kollef Marin H. , Govindan Ramaswamy , Westervelt Peter , Vranas Kelly C. , Maddox Thomas M. , Geng Elvin H. , Payne Philip R.O. , Politi Mary C. 
Published: January 2023

PURPOSE To elicit end-user and stakeholder perceptions regarding design and implementation of an inpatient clinical deterioration early warning system (EWS) for oncology patients to better fit routine clinical practices and enhance clinical impact. METHODS In an explanatory-sequential mixed-methods study, we evaluated a stakeholder-informed oncology early warning system (OncEWS) using surveys and semistructured interviews. Stakeholders were physicians, advanced practice providers (APPs), and nurses. For qualitative data, we used grounded theory and thematic content analysis via the constant comparative method to identify determinants of OncEWS implementation. RESULTS Survey respondents generally agreed that an oncology-focused EWS could add value beyond clinical judgment, with nurses endorsing this notion significantly more strongly than other clinicians (nurse: median 5 on a 6-point scale [6 = strongly agree], interquartile range 4-5; doctors/advanced practice providers: 4 [4-5]; P = .005). However, some respondents would not trust an EWS to identify risk accurately (n = 36 [42%] somewhat or very concerned), while others were concerned that institutional culture would not embrace such an EWS (n = 17 [28%]). Interviews highlighted important aspects of the EWS and the local context that might facilitate implementation, including (1) a model tailored to the subtleties of oncology patients, (2) transparent model information, and (3) nursing-centric workflows. Interviewees raised the importance of sepsis as a common and high-risk deterioration syndrome. CONCLUSION Stakeholders prioritized maximizing the degree to which the OncEWS is understandable, informative, actionable, and workflow-complementary, and perceived these factors to be key for translation into clinical benefit.
Hypofractionation in Breast Cancer Radiotherapy Across World Bank Income Groups: Results of an International Survey
Authors: Mushonga Melinda , Weiss Jessica , Liu Zhihui Amy , Nyakabau Anna-Mary , Mohamad Osama , Tawk Bouchra , Moraes Fabio Y. , Grover Surbhi , Yap Mei Ling , Zubizarreta Eduardo , Lievens Yolande , Rodin Danielle 
Published: January 2023

PURPOSE Hypofractionated breast radiotherapy has been found to be equivalent to conventional fractionation in many clinical trials. Using data from the European Society for Radiotherapy and Oncology Global Impact of Radiotherapy in Oncology survey, we identified preferences for hypofractionation in breast cancer across World Bank income groups and the perceived facilitators and barriers to its use. MATERIALS AND METHODS An international, electronic survey was administered to radiation oncologists from 2018 to 2019. Demographics, practice characteristics, preferred hypofractionation regimen for specific breast cancer scenarios, and facilitators and barriers to hypofractionation were reported and stratified by World Bank income groups. Variables associated with hypofractionation were assessed using multivariate logistic regression models. RESULTS One thousand four hundred thirty-four physicians responded: 890 (62%) from high-income countries (HICs), 361 (25%) from upper-middle–income countries (UMICs), 183 (13%) from low- and lower-middle–income countries (LLMICs). Hypofractionation was preferred most frequently in node-negative disease after breast-conserving surgery, with the strongest preference reported in HICs (78% from HICs, 54% from UMICs, and 51% from LLMICs, P < .001). Hypofractionation for node-positive disease postmastectomy was more frequently preferred in LLMICs (28% from HICs, 15% from UMICs, and 35% from LLMICs, P < .001). Curative doses of 2.1 to < 2.5 Gy in 15-16 fractions were most frequently reported, with limited preference for ultra-hypofractionation, but significant variability in palliative dosing. In adjusted analyses, UMICs were significantly less likely than LLMICs to prefer hypofractionation across all curative clinical scenarios, whereas respondents with > 1 million population catchments and with intensity-modulated radiotherapy were more likely to prefer hypofractionation. The most frequently cited facilitators and barriers were published evidence and fear of late toxicity, respectively. CONCLUSION Preference for hypofractionation varied for curative indications, with greater acceptance in earlier-stage disease in HICs and in later-stage disease in LLMICs. Targeted educational interventions and greater inclusivity in radiation oncology clinical trials may support greater uptake.
Expert Witness Testimony in Spine Surgery: A Review of Guidelines and Recommendations From Professional Organizations
Authors: Zhang Justin K. , Botterbush Kathleen S. , Mercier Philippe , Mattei Tobias A. 
Published: January 2023

BACKGROUND:
Most professional spine societies have enacted formal guidelines for spine surgeons providing expert witness services. However, there is significant heterogeneity in existing recommendations, with most societies providing information that is limited in detail and scope.

OBJECTIVE:
To provide a review of guidelines published by professional spine societies for spine surgeons serving as expert witnesses.

METHODS:
The Gale Directory Library, PubMed, and the grey literature were queried for national or international professional societies related to spine surgery. The search was focused on societies in the United States and North America, but also included well recognized international organizations in the field of spine surgery. Included societies with publicly available guidelines regarding expert witness services were extracted for 4 domains: (1) qualifications, (2) preparations, (3) testimony, and (4) compensation as well as the presence of a professional compliance program, defined as any official subcommittee aimed toward investigating claims of unethical behavior.

RESULTS:
Although most professional spine societies share general themes with respect to expert witness guidelines, important differences exist. Of the 26 societies included, 10 included publicly available guidelines: 4 of which were general neurosurgery societies, 2 general orthopedic surgery, and 4 spine specific. Three societies included the guidelines on all 4 domains (ie, qualifications, preparations, testimony, and compensation), and 2 societies included only 1 of the 4 domains. Eight societies possess a professional compliance program.

CONCLUSION:
There remains a paucity in expert witness guidelines provided by professional spine societies. Although existing recommendations are useful, there is a lack of standardized and comprehensive materials for spine surgeons providing expert witness testimony to reference. Moving forward, joint committees comprising surgeons, attorneys, and patient stakeholders may help improve the guidelines.
Optimal Timing of Cranioplasty and Predictors of Overall Complications After Cranioplasty: The Impact of Brain Collapse
Authors: Chen Renlong , Ye Gengzhao , Zheng Yan , Zhang Yuanlong , Zheng Shufa , Fang Wenhua , Mei Wenzhong , Xie Bingsen 
Published: January 2023

BACKGROUND:
The optimal timing of cranioplasty (CP) and predictors of overall postoperative complications are still controversial.

OBJECTIVE:
To determine the optimal timing of CP.

METHODS:
Patients were divided into collapsed group and noncollapsed group based on brain collapse or not, respectively. Brain collapse volume was calculated in a 3-dimensional way. The primary outcomes were overall complications and outcomes at the 12-month follow-up after CP.

RESULTS:
Of the 102 patients in this retrospective observation cohort study, 56 were in the collapsed group, and 46 were in the noncollapsed group. Complications were noted in 30.4% (n = 31), 24 (42.9%) patients in the collapsed group and 7 (15.2%) patients in the noncollapsed group, with a significant difference (P = .003). Thirty-three (58.9%) patients had good outcomes (modified Rankin Scale 0-3) in the collapsed group, and 34 (73.9%) patients had good outcomes in the noncollapsed group without a statistically significant difference (P = .113). Brain collapse (P = .005) and Karnofsky Performance Status score at the time of CP (P = .025) were significantly associated with overall postoperative complications. The cut-off value for brain collapse volume was determined as 11.26 cm3 in the receiver operating characteristic curve. The DC-CP interval was not related to brain collapse volume or postoperative complications.

CONCLUSION:
Brain collapse and lower Karnofsky Performance Status score at the time of CP were independent predictors of overall complications after CP. The optimal timing of CP may be determined by tissue window based on brain collapse volume instead of time window based on the decompressive craniectomy-CP interval.
Sero-epidemiology of brucellosis in small ruminants on rangeland in northern Mexico
Authors: Mellado M , Almanza A , Mellado J , García JE , Macías-Cruz U , Avendaño-Reyes L 
Published: January 2023

The seroprevalence of Brucella antibodies in serum of goats and sheep reared on semi-arid pastoral systems of northeastern Mexico was assessed. Additionally, species, gender, and predominant breed were evaluated as risk factors for seropositivity to brucellosis. Data were from 375 flocks of goats or co-mingled goats and sheep. Serum samples from 11001 goats and 4741 sheep collected between 2016 and 2019 were analyzed with the brucellosis card test. The disease affected 12% of flocks. The overall prevalence of brucellosis seropositivity in goats and sheep were estimated at 2.1% (95% CI = 1.78-2.31) and 0.8% (95% CI = 0.58-1.10), respectively. Brucellosis seropositivity was two times more likely (p < 0.01) in goats than sheep. The risk of seropositivity to brucellosis for goats and sheep was 2.3 and 3.2 times higher (p < 0.01) in females than males. Toggenburg goats were more likely (p < 0.01) to be seropositive to brucellosis than all other breeds of goats, whereas criollo sheep were more likely to be seropositive to brucellosis compared to hair sheep composite breeds. The current study revealed that, despite the absence of Brucella vaccination, brucellosis is not widely distributed in the study area. Also of all animals screened, seropositivity to Brucella infection was highest in Toggenburg when compared to other dairy and meat breeds; Criollo sheep also presented the highest seropositivity to brucellosis compared to hair sheep composite breeds. Finally, females compared to males had increased odds of testing positive for brucellosis.
The Impact of Chitosan on Oxidative Stress in Liver of Rats Loaded With Lead Acetate
Authors: Ozdek U , Toz H , Kömüroğlu AU , Mis L , Değer Y 
Published: January 2023

This study was conducted to determine the impact of chitosan on lead (Pb)-induced hepatotoxicity. Thus, lead acetate was administered intraperitoneally (50 mg/kg for 5 days) and chitosan was given as 200 mg/kg via oral gavage for 28 days. When the trial was terminated, it was determined that aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in serum as well as Pb, 8-hydroxy-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels and catalase (CAT) activity in liver tissue significantly increased, however reduced glutathione (GSH) and ceruloplasmin (Cp) levels in liver and high density lipoproteins (HDL) in serum had a significant decrease in the Pb group when compared to the control group. The administration of chitosan significantly prevented Pb-induced changes in serum liver enzyme activities, 8-OHdG and MDA levels. In addition, chitosan showed a statistically insignificant effect on reduced GSH, Cp, HDL levels and CAT activity. Accordingly, administration of chitosan can strengthen the antioxidant defence system of liver tissue and may decrease oxidative stress.
A comparative assessment of two different methods, using devices Optiscan-TP and Optigrade-MCP, for predicting the lean meat percentage of pig carcasses in Greece in relation to the European reference method
Authors: Schina V , Dotas V , Gourdouvelis D , Kaimakamis I , Mitsopoulos I , Tsakanikas G , Bampidis V 
Published: January 2023

Lean Meat Percentage (LMP) is a key index for assessing pork carcass quality as well as determining carcass classification and price. This framework offers to farmers the incentive to achieve the desired organoleptic features; therefore LMP must be measured objectively online, by means of various measuring instruments. The aim of this work was to assess two different methods for predicting the lean meat percentage of pig carcasses in Greece in comparison to the European reference method. For this reason, the same 130 pig carcasses (65 males and 65 females) were measured using 2 prediction devices (Optiscan-TP and Optigrade-MCP) and subsequently were assessed according to European reference method. A formula corresponding to each device was constructed using the ordinary least squares (OLS)method and excluding the same outliers. Calculated RMSEP for all 130 carcasses was 2.46518 for Optiscan-TP and 2.48489 for Optigrade-MCP. It can be concluded that both methods perform entirely according to EU legislation (Commission Regulation (EC) 1249/2008, European Union, 2008), and results obtained are similar regardless of different measurement points and technologies.
Phenotypic and genotypic characterization of antimicrobial resistance of Campylobacterisolates from poultry meat in Turkey
Authors: Can HY 
Published: January 2023

Phenotypic and genotypic antimicrobial resistances of Campylobacter strains from chicken meat were investigated. A total of 19 isolates (Campylobacter jejuni=6 and Campylobacter coli=13) were identified by multiplex PCR. The C. jejuni and C. coli isolates were tested for resistance to ciprofloxacin, erythromycin, and tetracycline using the disc diffusion method. In addition, all the strains were analyzed for resistance gene tet-O and mutations in the gyrA and 23S rRNA genes by PCR. Overall, a high frequency of resistance was detected against tetracycline (14/19, 73.6%), followed by erythromycin (7/19, 36.8%), and ciprofloxacin (6/19, 31.5%). Two of C. coli isolates (15.3%) were multidrug resistant, whereas none of the C. jejuni isolates were resistant to three antibiotics at the same time. Ten of the 14 Campylobacter strains (71.4%) resistant to tetracycline carried tet-O gene. Mutations in the 23S rRNA and gyrA genes were identified in 57.1% (4/7), 66.6% (4/6) of the isolates resistant to erythromycin and ciprofloxacin, respectively.