bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of sex and age on facial shape directional asymmetry in adults: A 3D landmarks-based method study
Authors: Harnádková Katarína , Kočandrlová Karolina , Kožejová Jaklová Lenka , Dupej Ján , Velemínská Jana 
Published: August 2023

ObjectivesFacial directional asymmetry research, including age-related changes, is crucial for the evaluation of treatment of craniofacial malformations/trauma in orthodontics, facial surgery and forensic sciences. The aim was to describe facial directional asymmetry (DA) in different age categories of adults using 3D methods. According to our hypothesis, facialshape DA(1) depends on sex; (2) differs among age groups; and (3) has wider variability in older age.Material and methodsA cross-sectional sample of healthy Czech adults without craniofacial trauma or anomalies consisted of 300 3D facial models (151 females). The age-range in the study was between 20–80 years. The shape asymmetry of 28 3D landmarks was evaluated using geometric morphometrics and multivariate statistics.ResultsThe manifestation of DA was similar in both sexes and in each age category; however, there were some statistical differences. In contrast to the ideal symmetrical face, the mean asymmetrical faces tended to create a slightly bent “C” shape of the midline. Therefore, the upper face was rotated slightly clockwise and the lower face counter-clockwise. The right eye was located slightly higher, with the nasal tip and mandibular region tilting to the left. Sex differences in facial DA were significant before the age of 40. DA was more significant in the youngest males than in the oldest, while the women’s DA did not change.ConclusionsThe DA patterns were similar in both sexes and in all age categories (a slightly bent C shape of the midline); however, some significant local differences between male age groups were found. A significantly more pronounced asymmetry compared to other age groups was found only in the youngest males from 20 to 40 years. Moreover, significant sexual dimorphism of DA rapidly decreased after middle age, likely caused by the same age-related changes of the face during aging.
An approach to difficult airway in infants: Comparison of GlideScope® Spectrum LoPro, GlideScope® Spectrum Miller and conventional Macintosh and Miller blades in a simulated Pierre Robin sequence performed by 90 anesthesiologists
Authors: Irouschek Andrea , Moritz Andreas , Kremer Sven , Fuchte Tobias , Danzl Anja , Schmidt Joachim , Golditz Tobias 
Published: August 2023

Background
Airway management can be challenging in neonates and infants. The Pierre Robin sequence (PRS) is a condition characterized by micrognathia, glossoptosis and airway obstruction. The airway management of these patients poses great challenges for anesthesiologists and pediatricians alike. To date, there has been no direct comparison of the hyperangulated GlideScope® Spectrum LoPro (GLP), the straight GlideScope® Spectrum Miller (GSM), a conventional Macintosh (MC) and a conventional Miller blade (ML) in patients with PRS.

Methods
For this purpose, 90 anesthesiologists (43 with limited experience, 47 with extensive experience) performed orotracheal intubation on an Air-Sim® Pierre Robin X manikin using GLP, GSM, MC and ML in randomized order. ‘Time-to-vocal-cords’, ‘time-to-intubate’, ‘time-to-ventilate’, the severity of oral-soft-tissue-trauma and the subjective evaluation of each device were recorded.

Results
A significantly faster and better view of the vocal cords and lower oral-soft-tissue-trauma was achieved using the GLP (p<0.001). Though, there were no significant differences in the ‘time-to-intubate’ or ‘time-to-ventilate’. The highest intubation success rate was found with GSM and the lowest with GLP (GSM 100%, ML 97.8%, MC 96.7%, GLP 93.3%). When using the videolaryngoscopes, there were no undetected esophageal intubations but in six cases prolonged attempts of intubation (>120s) with the GLP. In the sub-group with extensive experience, we found significantly shorter intubation times for the GSM and ML. The GLP was the tool of choice for most participants, while the conventional MC received the lowest rating.

Conclusions
Videolaryngoscopy leads to increased safety for the prevention of undetected esophageal intubation in the airway management in a PRS manikin. Hyperangulated blades may ensure a good and fast view of the vocal cords and low oral-soft-tissue-trauma but pose a challenge during the placement of the tube. Specific skills and handling seem to be necessary to ensure a safe tube placement with this sort of blades.
Point-of-care PCR testing of SARS-CoV-2 in the emergency department: Influence on workflow and efficiency
Authors: Fistera David , Kikull Katja , Risse Joachim , Herrmann Anke , Brachmann Matthias , Kill Clemens 
Published: August 2023

Problem
Regarding transmissible viral diseases such as those caused by SARS-CoV-2 virus, one of the key challenges is isolation management until final diagnosis.
This study investigates the influence of SARS-CoV-2 point-of-care (POC) PCR on workflow and efficiency in an emergency department (ED) of a tertiary university hospital.

Method
An analysis of 17,875 ED patients receiving either SARS-CoV-2 POC PCR (rapid PCR, 11,686 patients) or conventional laboratory SARS-CoV-2 PCR (conventional PCR, 6,189 patients) was performed. The pathways for both groups were mapped and compared, and process times from admission to diagnosis were measured. Effects on resource management within the ED were quantified. Direct costs due to isolation, loss of capacities, and revenues were calculated for inpatients.

Results
The mean time from admission to result was 1.62 h with rapid PCR and 16.08 h with conventional PCR (p < 0.01), reducing the isolation time by 14.46 h. In the first 2 h after testing, test results were available for > 75% of the rapid PCR group and none of the conventional PCR group. Ninety percent of the results were available within 3 h for the rapid PCR and within 21 h for the conventional PCR group. For the conventional PCR group, an increase in direct costs of €35.74 and lost revenues of €421.06 for each inpatient case was detected.

Conclusion
Rapid PCR significantly reduces the time-to-results and time for isolation relative to conventional PCR. Although testing costs for rapid PCR are higher, it benefits workflow, reduces total costs, and frees up ward capacity.
Effect of veterinary feed directive rule changes on tetracycline-resistant and erythromycin-resistant bacteria (Salmonella, Escherichia, and Campylobacter) in retail meats in the United States
Authors: Sarkar Shamim , Okafor Chika 
Published: August 2023

Background
Antimicrobial-resistant bacteria are a growing public health threat. In 2017 the U.S. Food and Drug Administration implemented Veterinary Feed Directive (VFD) rules changes to limit medically important antimicrobial use in food-producing animals, combating antimicrobial-resistant bacteria. The effect of the VFD rule changes on the occurrence of bacteria resistant to medically-important antimicrobials in retail meats is yet to be investigated in the U.S. This study investigates whether the VFD rule changes affected the occurrence of tetracycline-resistant and erythromycin-resistant bacteria (Salmonella, Escherichia, and Campylobacter) in retail meats in the U.S.

Methods
Multivariable mixed effect logistic regression models were used to analyze 2002–2019 retail meats surveillance data from the National Antimicrobial Resistance Monitoring System (NARMS) in the U.S. Variables included VFD rule changes, meat type, quarter of year, and raising claims. A potential association between these variables and the occurrence of tetracycline-resistant and erythromycin-resistant bacteria (Salmonella, Escherichia, and Campylobacter) in retail meats was estimated.

Results
Analysis included data regarding tetracycline-resistant Salmonella (n = 8,501), Escherichia (n = 20, 283), Campylobacter (n = 9,682), and erythromycin-resistant Campylobacter (n = 10,446) in retail meats. The odds of detecting tetracycline-resistant Escherichia (OR = 0.60), Campylobacter (OR = 0.89), and erythromycin-resistant Campylobacter (OR = 0.43) in chicken breast significantly decreased after the VFD rule changes, compared to the pre-VFD rule change period. The odds of detecting tetracycline-resistant Salmonella (0.66), Escherichia (OR = 0.56), and Campylobacter (OR = 0.33) in ground turkey also significantly decreased. However, the odds of detecting tetracycline-resistant Salmonella (OR = 1.49) in chicken breast and erythromycin-resistant Campylobacter (OR = 4.63) in ground turkey significantly increased. There was no significant change in the odds of detecting tetracycline-resistant Salmonella and Escherichia in ground beef or pork chops.

Conclusions
The implementation of VFD rule changes had a beneficial effect by reducing the occurrence of tetracycline-resistant and erythromycin-resistant bacteria in chicken and ground turkey. Ongoing surveillance of antimicrobial resistance and antimicrobial use could complement the implementation of stewardship such as VFD rule in food-producing animals in the U.S.
Evaluating the ability of an artificial-intelligence cloud-based platform designed to provide information prior to locoregional therapy for breast cancer in improving patient’s satisfaction with therapy: The CINDERELLA trial
Authors: Kaidar-Person Orit , Antunes Marilia , Cardoso Jaime S. , Ciani Oriana , Cruz Helena , Di Micco Rosa , Gentilini Oreste D. , Gonçalves Tiago , Gouveia Pedro , Heil Jörg , Kabata Pawel , Lopes Daniela , Martinho Marta , Martins Henrique , Mavioso Carlos , Mika Martin , Montenegro Helena , Oliveira Helder P. , Pfob André , Rotmensz Nicole , Schinköthe Timo , Silva Giovani , Tarricone Rosana , Cardoso Maria-Joao ,   on behalf of the CINDERELLA Consortium
Published: August 2023

Background
Breast cancer therapy improved significantly, allowing for different surgical approaches for the same disease stage, therefore offering patients different aesthetic outcomes with similar locoregional control. The purpose of the CINDERELLA trial is to evaluate an artificial-intelligence (AI) cloud-based platform (CINDERELLA platform) vs the standard approach for patient education prior to therapy.

Methods
A prospective randomized international multicentre trial comparing two methods for patient education prior to therapy. After institutional ethics approval and a written informed consent, patients planned for locoregional treatment will be randomized to the intervention (CINDERELLA platform) or controls. The patients in the intervention arm will use the newly designed web-application (CINDERELLA platform, CINDERELLA APProach) to access the information related to surgery and/or radiotherapy. Using an AI system, the platform will provide the patient with a picture of her own aesthetic outcome resulting from the surgical procedure she chooses, and an objective evaluation of this aesthetic outcome (e.g., good/fair). The control group will have access to the standard approach. The primary objectives of the trial will be i) to examine the differences between the treatment arms with regards to patients’ pre-treatment expectations and the final aesthetic outcomes and ii) in the experimental arm only, the agreement of the pre-treatment AI-evaluation (output) and patient’s post-therapy self-evaluation.

Discussion
The project aims to develop an easy-to-use cost-effective AI-powered tool that improves shared decision-making processes. We assume that the CINDERELLA APProach will lead to higher satisfaction, better psychosocial status, and wellbeing of breast cancer patients, and reduce the need for additional surgeries to improve aesthetic outcome.
Identification and validation of a m7G-related lncRNA signature for predicting the prognosis and therapy response in hepatocellular carcinoma
Authors: Peng Yue-Ling , Dong Ya-Fang , Guo Li-Li , Li Mu-Ye , Liao Hui , Li Rong-Shan 
Published: August 2023

Background
N7-methylguanosine (m7G) is one of the most common RNA posttranscriptional modifications; however, its potential role in hepatocellular carcinoma (HCC) remains unknown. We developed a prediction signature based on m7G-related long noncoding RNAs (lncRNAs) to predict HCC prognosis and provide a reference for immunotherapy and chemotherapy.

Methods
RNA-seq data from The Cancer Genome Atlas (TCGA) database and relevant clinical data were used. Univariate and multivariate Cox regression analyses were conducted to identify m7G-related lncRNAs with prognostic value to build a predictive signature. We evaluated the prognostic value and clinical relevance of this signature and explored the correlation between the predictive signature and the chemotherapy treatment response of HCC. Moreover, an in vitro study to validate the function of CASC19 was performed.

Results
Six m7G-related lncRNAs were identified to create a signature. This signature was considered an independent risk factor for the prognosis of patients with HCC. TIDE analyses showed that the high-risk group might be more sensitive to immunotherapy. ssGSEA indicated that the predictive signature was strongly related to the immune activities of HCC. HCC in high-risk patients was more sensitive to the common chemotherapy drugs bleomycin, doxorubicin, gemcitabine, and lenalidomide. In vitro knockdown of CASC19 inhibited the proliferation, migration and invasion of HCC cells.

Conclusion
We established a 6 m7G-related lncRNA signature that may assist in predicting the prognosis and response to chemotherapy and immunotherapy of HCC.
Trait anxiety and depressive rumination mediate the effect of perceived childhood rearing on adulthood presenteeism
Authors: Shimasaki Akifumi , Deguchi Ayaka , Ishii Yoshitaka , Seki Tomoteru , Iwata Yoshio , Tamada Yu , Honyashiki Mina , Fujimura Yota , Inoue Takeshi , Masuya Jiro 
Published: August 2023

Aim
Productivity loss in the workplace due to physical or mental health problems, which is called presenteeism, leads to large financial losses. Personal and work environment factors, as well as physical and mental illnesses are associated with presenteeism, but the detailed underlying mechanism remains unclear. In this study, we analyzed the effects of perceived childhood rearing on the presenteeism of adult workers, and the mediating effects of trait anxiety and depressive rumination.

Methods
In 2017 and 2018, a cross-sectional paper-based survey was conducted, and written consent from 447 adult workers was obtained. Demographic information and results from the Parental Bonding Instrument, trait anxiety of State-Trait Anxiety Inventory Form Y, Ruminative Responses Scale, and Work Limitations Questionnaire were surveyed. Multiple regression analyses and structural equation modeling were conducted.

Results
The low perceived quality of rearing from parents in childhood, i.e., low care and high overprotection, indirectly worsened current presenteeism via trait anxiety and depressive rumination. Presenteeism was directly worsened by trait anxiety and depressive rumination, and the low perceived quality of rearing from parents directly affected trait anxiety and depressive rumination, and trait anxiety affected depressive rumination.

Conclusion
The results of this study clarified the long-term influences of the low perceived quality of rearing experienced in childhood on adulthood presenteeism via trait anxiety and depressive rumination. Therefore, assessing the quality of childhood rearing, trait anxiety, and depressive rumination of individuals may help to elucidate the causes of presenteeism in the workplace, and how to manage it effectively.
Defeating a superbug: A breakthrough in vaccine design against multidrug-resistant Pseudomonas aeruginosa using reverse vaccinology
Authors: Fereshteh Sepideh , Haririzadeh Jouriani Fatemeh , Noori Goodarzi Narjes , Torkamaneh Mahdi , Khasheii Behnoush , Badmasti Farzad 
Published: August 2023

Background
Multidrug-resistant Pseudomonas aeruginosa has become a major cause of severe infections. Due to the lack of approved vaccines, this study has presented putative vaccine candidates against it.

Methods
P. aeruginosa 24Pae112 as a reference strain was retrieved from GenBank database. The surface-exposed, antigenic, non-allergenic, and non-homologous human proteins were selected. The conserved domains of selected proteins were evaluated, and the prevalence of proteins was assessed among 395 genomes. Next, linear and conformational B-cell epitopes, and human MHC II binding sites were determined. Finally, five conserved and highly antigenic B-cell epitopes from OMPs were implanted on the three platforms as multi-epitope vaccines, including FliC, the bacteriophage T7 tail, and the cell wall-associated transporter proteins. The immunoreactivity was investigated using molecular docking and immune simulation. Furthermore, molecular dynamics simulation was done to refine the chimeric cell-wall-associated transporter-TLR4 complex as the best interaction.

Results
Among 6494 total proteins of P. aeruginosa 24Pae112, 16 proteins (seven OMPs and nine secreted) were ideal according to the defined criteria. These proteins had a molecular weight of 110 kDa and were prevalent in ≥ 75% of P. aeruginosa genomes. Among the presented multi-epitope vaccines, the chimeric cell-wall-associated transporter had the strongest interaction with TLR4. Moreover, the immune simulation response revealed that the bacteriophage T7 tail chimeric protein had the strongest ability to stimulate the immune system. In addition, molecular docking and molecular dynamic simulation indicated the proper and stable interactions between the chimeric cell-wall-associated transporter and TLR4.

Conclusion
This study proposed 16 shortlisted proteins as promising immunogenic targets. Two novel platforms (e.g. cell-wall-associated transporter and bacteriophage T7 tail proteins) for designing of multi-epitope vaccines (MEVs), showed the better performance compared to FliC. In our future studies, these two MEVs will receive more scrutiny to evaluate their immunoreactivity.
Status and distribution of the Eurasian lynx (Lynx lynx L.) in Slovenia in 2000–2004 and comparison with the years 1995–1999
Authors: Koren Iztok , Jonozovič Marko , Kos Ivan , Skrbinšek Tomaž 
Published: August 2023

We have analysed recorded signs of lynx presence in Slovenia for the period 2000 – 2004 and compared them with the 1995 – 1999 period to determine population status, trends and range.
The analysis included 908 recorded signs of lynx presence, which is an 80% increase compared to the previous ﬁve-year period. The lynx monitoring has improved, both in the total number of acquired data, as well as in the share of the higher-reliability data. With regard to lynx presence, Slovenia can be divided into four areas: (1) the southern part, the area south of the Trieste–Ljubljana–Zagreb motorway (Kočevska and Notranjska regions), the area to which the lynx was ﬁrst reintroduced and where the majority of the lynx in Slovenia are still present today, (2) the north-western part of the country with Julian Alps, the area that the lynx started to colonize in the mid eighties of the previous century, (3) Kamnik–Savinja Alps and some other, isolated areas with occasional lynx presence, (4) other areas (North-eastern and Eastern Slovenia), where lynx are not present. Based on the collected data we estimate there are 30 – 50 animals of this species present in Slovenia, 15 of which live in the western part of the country. The size of the lynx range has not decreased over the last ﬁve years, and the number of damage cases has increased. Compared to the previous period the status of the lynx population remained unchanged during the 2000 – 2004 period, and so the Slovenian population still remains one of the most vital populations in the Alps.
La sobreexpresión de FoxO1 en el hígado esta positivamente asociada al grado de daño hepático en pacientes cirróticos
Authors: Fernández-Galán Esther , Sandalinas Silvia , Macias-Muñoz Laura , Portolés Irene , Ribera Jordi , Morales-Romero Blai , Pauta Montse , Casals Gregori , Boix Loreto , Jiménez Wladimiro , Morales-Ruiz Manuel 
Published: August 2023

Resumen

Objetivos
La enfermedad hepática crónica y sus complicaciones, la cirrosis y el carcinoma hepatocelular, presentan una elevada mortalidad. Los tratamientos curativos, como la hepatectomía parcial o el trasplante hepático, tienen una aplicación limitada en pacientes con cirrosis, por su escasa capacidad de regeneración hepática. Son necesarias otras alternativas diagnósticas y terapéuticas para prevenir la progresión de la enfermedad y mejorar la supervivencia. Diversos estudios preclínicos demuestran el importante papel de la proteína quinasa B(Akt) en la disfunción hepática, aunque aún se desconoce el estado de Akt y sus dianas en las patologías hepáticas crónicas. El principal objetivo es determinar el estado de activación de la vía Akt y su relación con la función hepática en pacientes cirróticos.

Métodos
Estudio retrospectivo con muestras de tejido hepático de 36 pacientes hepatectomizados con (n=27) y sin (n=9) cirrosis. Se realizó un análisis Multiplex de las proteínas de la vía Akt/mTOR empleando un panel Luminex y Western blot. Previamente a la resección, se realizaron las pruebas habituales de función hepática en suero.

Resultados
Akt y la proteína FoxO1 están sobreexpresadas en el hígado cirrótico: 1.0 unidades densitométricas relativas (UDR); p<0,01, y 9,5 vs. 4,4 DRU; p<0,01, respectivamente). FoxO1 mostró una fuerte correlación con los marcadores de daño hepático (aspartato aminotransferasa (ASAT): r=0,51, p<0,05; alanina aminotransferasa (ALAT): r=0,49, p<0,05), y fue la única enzima de la vía Akt identificada como predictor independiente de los niveles de ASAT y ALAT.

Conclusiones
La expresión intrahepática de FoxO1 podría tener utilidad clínica como marcador pronóstico en la enfermedad hepática avanzada.
Present perfect and preterit variation in the Spanish of Lima and Mexico city: findings from a corpus analysis
Authors: Mastrantuono Anna , Regan Brendan 
Published: August 2023

AbstractIn many languages, the present perfect has grammaticalized, gradually displacing the preterit. Within Spanish, this has been documented with the grammaticalization of the present perfect in Peninsular Spanish. To examine this possibility in two Latin American varieties, this study examined present perfect/preterit variation of 36 speakers from Lima and Mexico City from the PRESEEA corpus. While Lima Spanish presented overall more present perfect than Mexico City Spanish, a similar internal constraint hierarchy is predictive of present perfect use in both speech communities. However, Lima Spanish demonstrated a change in progress toward an expansion of the preterit among younger speakers with the indeterminate temporal reference as locus of change. The findings suggest that present perfect grammaticalization may not always be the most common cross-linguistic pathway but rather is subject to source constraints, which may lead to another pathway in which the preterit expands at the expense of the present perfect.