bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphometry and mechanical instability at the onset of epithelial bladder cancer
Authors: Lampart Franziska L. , Vetter Roman , Yamauchi Kevin A. , Wang Yifan , Runser Steve , Strohmeyer Nico , Meer Florian , Hussherr Marie-Didiée , Camenisch Gieri , Seifert Hans-Helge , Rentsch Cyrill A. , Le Magnen Clémentine , Müller Daniel J. , Bubendorf Lukas , Iber Dagmar 
Published: January 2025

Abstract
Malignancies of epithelial tissues, called carcinomas, account for most cancer cases. Research has largely focused on correlating different carcinoma subtypes to genetic alterations. However, as well as a rewiring in the signalling networks, carcinoma progression is accompanied by mechanical changes in the epithelial cells and the extracellular matrix. Here we reveal intricate morphologies in the basement membrane at the onset of bladder cancer and propose that they emerge from a mechanical instability upon epithelial overgrowth. We imaged mouse and human bladder tissue and performed differential growth simulations, and found that stiffness changes in the different mucosa layers can result in aberrant tissue morphologies. The resulting thickening, wrinkles and folds resemble early papillary tumours and carcinomas in situ. Atomic force microscopy confirmed local stiffness changes in the pathological basement membrane. Our findings suggest a possible mechanical origin of the different bladder carcinoma subtypes and may guide future developments in treatment and prophylaxis.
Intracerebroventricular B7-H3-targeting CAR T cells for diffuse intrinsic pontine glioma: a phase 1 trial
Authors: Vitanza Nicholas A. , Ronsley Rebecca , Choe Michelle , Seidel Kristy , Huang Wenjun , Rawlings-Rhea Stephanie D. , Beam Madison , Steinmetzer Leonel , Wilson Ashley L. , Brown Christopher , Beebe Adam , Lindgren Catherine , Gustafson Joshua A. , Wein Amy , Holtzclaw Susan , Hoeppner Corrine , Goldstein Hannah E. , Browd Samuel R. , Hauptman Jason S. , Lee Amy , Ojemann Jeffrey G. , Crotty Erin E. , Leary Sarah E. S. , Perez Francisco A. , Wright Jason N. , Alonso Marta M. , Dun Matthew D. , Foster Jessica B. , Hurst Diana , Kong Ada , Thomsen Alison , Orentas Rimas J. , Albert Catherine M. , Pinto Navin , Annesley Colleen , Gardner Rebecca A. , Ho On , Pattabhi Sowmya , Gust Juliane , Wendler Jason P. , Park Julie R. , Jensen Michael C. 
Published: January 2025

AbstractDiffuse intrinsic pontine glioma (DIPG) is a fatal central nervous system (CNS) tumor that confers a median survival of 11 months. As B7-H3 is expressed on pediatric CNS tumors, we conducted BrainChild-03, a single-center, dose-escalation phase 1 clinical trial of repetitive intracerebroventricular (ICV) dosing of B7-H3-targeting chimeric antigen receptor T cells (B7-H3 CAR T cells) for children with recurrent or refractory CNS tumors and DIPG. Here we report results from Arm C, restricted to patients with DIPG. The primary objectives were to assess feasibility and tolerability, which were both met. Secondary objectives included assessments of CAR T cell distribution and survival. A total of 23 patients with DIPG enrolled, and 21 were treated with repeated doses of ICV B7-H3 CAR T cells using intra-patient dose-escalation regimens without previous lymphodepletion. Concurrent tumor-directed therapy, including re-irradiation, was not allowed while on protocol therapy. We delivered a total of 253 ICV doses and established the highest planned dose regimen, DR4, which escalated up to 10 × 107 cells per dose, as the maximally tolerated dose regimen. Common adverse events included headache, fatigue and fever. There was one dose-limiting toxicity (intratumoral hemorrhage) during DR2. For all treated patients (n = 21), the median survival from their initial CAR T cell infusion was 10.7 months and the median survival from diagnosis was 19.8 months with 3 patients still alive at 44, 45 and 52 months from diagnosis. Ultimately, this completed first-in-human trial shows that repetitive ICV dosing of B7-H3 CAR T cells in pediatric and young adult patients with DIPG is tolerable, including multiyear repeated dosing, and may have clinical efficacy that warrants further investigation on a multisite phase 2 trial. ClinicalTrials.gov registration: NCT04185038.
Hydrocarbon potential in the Northern Egyptian Red Sea: insights from geophysical datasets and analysis of onshore marginal outcrop analogues and subsurface sequences
Authors: Noureldin Ahmed M. , Hassan Mustafa , Farouk Mohamed , Mabrouk Walid M. , Radwan Ahmed E. , Metwally Ahmed 
Published: January 2025

AbstractThe Red Sea remains a largely under-explored basin, with the Northern Egyptian Red Sea requiring further investigation due to limited borehole data, sparse case studies, and poor seismic quality. A petroleum system, regional structural cross-section, and geological block diagrams integrating onshore fieldwork from Gebel Duwi and offshore subsurface geology were utilized to assess the hydrocarbon potential of the Northern Egyptian Red Sea (NERS). The findings highlight that pre- and syn-rift organic-rich source units in the NERS could generate oil and gas, similar to the capped reservoirs of the Southern Gulf of Suez. The study also reveals that both regions (NERS and SGOS) were influenced by the Levant-Aqaba transform fault influenced both regions (NERS and SGOS), resulting in southwest-dipping strata, replicating the Northern Gulf of Suez structural style. Additionally, rifting extended the rotated basement faulted blocks from the shoreline to the axial trough, creating wedged Miocene sections. Most offshore boreholes in the Red Sea bottomed in Precambrian rock directly below the syn-rift Miocene sequence, suggesting these wells were off-structure, similar to sidetracked wells in the Gulf of Suez, which successfully captured thick pre- and syn-rift sediments. These insights can drive further advancements in hydrocarbon exploration in the Northern Egyptian Red Sea.
Ovario- protective effect of Moringa oleifera leaf extract against cyclophosphamide-induced oxidative ovarian damage and reproductive dysfunction in female rats
Authors: Soliman Seham Samir , Suliman Ahmed A. , Fathy Khaled , Sedik Ahmed A. 
Published: January 2025

AbstractIt is crucial to develop new tactics to prevent ovarian tissue damage in women whose reproductive toxicity is caused by chemotherapy. The present investigation was performed to assess the protective effects of Moringa oleifera (M. oleifera) leaf extract on cyclophosphamide (CP)-induced ovarian damage and reproductive dysfunction. Thirty-two female, healthy Wistar albino rats were randomly assigned to four groups (8 rats/group). The first group was given saline intraperitoneally (i.p.). The second group was given a single dose of CP (200 mg/kg; i.p.). The third and fourth groups were given M. oleifera leaf extract (150 and 250 mg/kg; orally) for 20 days before receiving CP on the final day of the experiment. Hormonal assessments, including follicle stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (ES), were performed 24 h after CP administration. In addition, the antioxidant status and inflammatory response against CP were evaluated. Moreover, detailed histopathological and ultra- structural observations were conducted. For evaluation of statistical significance between different groups; One-way analysis of variance (ANOVA) with Tukey’s post hoc test was adopted. Our findings revealed that rats subjected to CP showed increased levels of FSH, LH, malondialdehyde (MDA), tumor necrosis factor-alpha, and interleukin-8 and decreased levels of ES and glutathione. Pre-treatment with M. oleifera leaf extract (250 mg/kg; orally) was statistically significant (p values < 0.05) as it could improve hormonal changes, oxidative stress indices, and pro- inflammatory mediator levels. Consequently, a marked improvement was observed in the ovarian and uterine architectures, with a normal ovarian reserve and a normal endothelium with normal tubular glands. In conclusion, M. oleifera leaf extract (250 mg/kg) could be used as a pharmaceutical supplement because it protects female rats against CP-induced ovarian damage and reproductive dysfunction.
Patterns and implications of spatial covariation in herbivore functions on resilience of coral reefs
Authors: Cook Dana T. , Holbrook Sally J. , Schmitt Russell J. 
Published: January 2025

AbstractPersistent shifts to undesired ecological states, such as shifts from coral to macroalgae, are becoming more common. This highlights the need to understand processes that can help restore affected ecosystems. Herbivory on coral reefs is widely recognized as a key interaction that can keep macroalgae from outcompeting coral. Most attention has been on the role ‘grazing’ herbivores play in preventing the establishment of macroalgae, while less research has focused on the role of ‘browsers’ in extirpating macroalgae. Here we explored patterns, environmental correlates and state shift consequences of spatial co-variation in grazing and browsing functions of herbivorous fishes. Grazing and browsing rates were not highly correlated across 20 lagoon sites in Moorea, French Polynesia, but did cluster into 3 (of 4) combinations of high and low consumption rates (no site had low grazing but high browsing). Consumption rates were not correlated with grazer or browser fish biomass, but both were predicted by specific environmental variables. Experiments revealed that reversibility of a macroalgal state shift was strongly related to spatial variation in browsing intensity. Our findings provide insights and simple diagnostic tools regarding heterogeneity in top-down forcing that influences the vulnerability to and reversibility of shifts to macroalgae on coral reefs.
Identification of new reference genes with stable expression patterns for cell cycle experiments in human leukemia cell lines
Authors: Tóth Otília , Rácz Gergely Attila , Oláh Eszter , Tóth Máté , Szabó Edit , Várady György , Vértessy Beáta G. , Nagy Nikolett 
Published: January 2025

AbstractCell cycle-dependent gene expression analysis is particularly important as numerous genes show tightly regulated expression patterns at different phases of the cell cycle. For cancer cells, analysis of cell cycle-related events is of paramount significance since tumorigenesis is characteristically coupled to cell cycle perturbations. RT-qPCR is a highly sensitive technique to investigate cell cycle-dependent transcriptional regulation. However, for reliable evaluation of qPCR data reference genes with stable mRNA expression are required. Although several studies investigating cell cycle-dependent gene expression employ frequently used reference genes, the suitability of these reference genes has not been thoroughly investigated so far. Moreover, such potential reference genes for cell cycle analysis have not been described in the literature. Therefore, we aimed to identify reference genes characterized with stable expression throughout the cell cycle in MOLT4 and U937 human leukemia cell lines synchronized with RO-3306 CDK1 inhibitor using RT-qPCR. Here we show that for cell cycle-dependent gene expression analysis the commonly used TBP is suitable, while the recently recognized reference genes SNW1 and CNOT4 are applicable in a cell line-dependent manner. We also suggest that proper selection of reference genes for each experimental condition is crucial for reliable normalization as these aspects can severely compromise conclusions.
Holographic inspired high-performance circular polarized spoof surface plasmon polariton leaky-wave antenna excited by a novel launcher
Authors: Zohrevand Sajjad , Chaychi Zadeh Mohammad Amin , Farokhipour Ehsan , Erni Daniel , Komjani Nader 
Published: January 2025

AbstractThe holographic technique is one of the simplest methods for designing antennas based on metasurface. This paper presents a spoof surface plasmon polariton (SSPP) leaky-wave antenna (LWA) based on the concept of impedance modulated metasurfaces by the anisotropic holographic technique. Instead of parasitic elements, anisotropic SSPP elements are exploited to achieve radiation with circular polarization. The characteristics of the SSPP elements are obtained by the aperture field estimate method. The hologram surface consists of hollow cross-bars unit cells. The anisotropy of each unit cell is achieved by combining the transformation optic method and the particle swarm optimization algorithm. A major challenge of the SSPP LWA based on modulated impedance surfaces is to find a suitable excitation technique. This study proposes a waveguide strip line launcher for excitation to minimize interference on the radiation pattern beam. The designed launcher provides a good impedance matching from 8 to 20 GHz, with an impedance bandwidth of 142%. The peak gain, radiation efficiency, axial ratio (AR) bandwidth, and side lobe level at the design frequency of 18 GHz are 19.7dBi, 93%, 11%, and − 12.1 dB, respectively. After optimizations and simulations are conducted using MATLAB and CST software, the proposed antenna is fabricated, and its radiation characteristics are measured. The measured results agree well with the simulated ones, indicating the high validity of the method.
Potential of CoMn2O4 spinel as soot oxidation catalyst and its kinetics thereof
Authors: Nithya R. , Patil Sunaina S. , Dasari Hari Prasad , Dasari Harshini , Nethaji S. 
Published: January 2025

AbstractEfficient catalysts for soot oxidation are critical for mitigating environmental pollution. In this study, CoMn2O4 spinel catalysts were synthesised using reverse co-precipitation and co-precipitation methods to evaluate their performance in soot oxidation and kinetic behaviour. All samples exhibited a tetragonal phase (XRD) and spherical morphology with rough surfaces (SEM). Raman spectroscopy confirmed structural disorder and oxygen vacancies, while XPS analysis revealed the presence of low-valence Mn ions, facilitating oxygen vacancy formation critical for soot oxidation. Additionally, the co-existence of Co and Mn ions contributed to a synergistic effect, enhancing the catalytic properties of the spinel structure. The reverse co-precipitation method produced a catalyst with a higher concentration of oxygen vacancies and active oxygen species among the samples. This sample demonstrated superior catalytic performance, achieving a T50% of 424 °C, low activation energy (153 kJ/mol) and pre-exponential factor (25 min− 1). Soot TPR analysis highlighted the role of catalyst reducibility, while thermogravimetric analysis revealed that activation energy and pre-exponential factors were influenced by surface composition. These findings provide valuable insights into the design of efficient catalysts for soot oxidation, emphasising the importance of synthesis methods and surface characteristics.
Simulated Antarctic sea ice expansion reconciles climate model with observation
Authors: Liu Wei 
Published: January 2025

AbstractObservations reveal Antarctic sea ice expansion and Southern Ocean surface cooling trends from 1979 to 2014, whereas climate models mostly simulate the opposite. Here I use historical ensemble simulations with multiple climate models to show that sea-ice natural variability enables the models to simulate an Antarctic sea ice expansion during this period under anthropogenic forcings. Along with sea-ice expansion, Southern Ocean surface and subsurface temperatures up to 50oS, as well as lower tropospheric temperatures between 60oS and 80oS, exhibit significant cooling trends, all of which are consistent with observations. Compared to the sea-ice decline scenario, Antarctic sea ice expansion brings tropical precipitation changes closer to observations. Neither the Southern Annular Mode nor the Interdecadal Pacific Oscillation can fully explain the simulated Antarctic sea ice expansion over 1979–2014, while the sea-ice expansion is closely linked to surface meridional winds associated with a zonal wave 3 pattern.
Functional brain network dynamics mediate the relationship between female reproductive aging and interpersonal adversity
Authors: Petrican Raluca , Chopra Sidhant , Segal Ashlea , Fallon Nick , Fornito Alex 
Published: January 2025

AbstractPremature reproductive aging is linked to heightened stress sensitivity and psychological maladjustment across the life course. However, the brain dynamics underlying this relationship are poorly understood. Here, to address this issue, we analyzed multimodal data from female participants in the Adolescent Brain and Cognitive Development (longitudinal, N = 441; aged 9–12 years) and Human Connectome-Aging (cross-sectional, N = 130; aged 36–60 years) studies. Age-specific intrinsic functional brain network dynamics mediated the link between reproductive aging and perceptions of greater interpersonal adversity. The adolescent profile overlapped areas of greater glutamatergic and dopaminergic receptor density, and the middle-aged profile was concentrated in visual, attentional and default mode networks. The two profiles showed opposite relationships with patterns of functional neural network variability and cortical atrophy observed in psychosis versus major depressive disorder. Our findings underscore the divergent patterns of brain aging linked to reproductive maturation versus senescence, which may explain developmentally specific vulnerabilities to distinct disorders.
Challenges and applications of artificial intelligence in infectious diseases and antimicrobial resistance
Authors: Cesaro Angela , Hoffman Samuel C. , Das Payel , de la Fuente-Nunez Cesar 
Published: January 2025

AbstractArtificial intelligence (AI) has transformed infectious disease control, enhancing rapid diagnosis and antibiotic discovery. While conventional tests delay diagnosis, AI-driven methods like machine learning and deep learning assist in pathogen detection, resistance prediction, and drug discovery. These tools improve antibiotic stewardship and identify effective compounds such as antimicrobial peptides and small molecules. This review explores AI applications in diagnostics, therapy, and drug discovery, emphasizing both strengths and areas needing improvement.
Voice Meets Swallowing: A Scoping Review of Therapeutic Connections
Authors: Castillo-Allendes Adrián , Searl Jeff , Vergara José , Ballentine Natalie , Ebdah Soud , Rameau Anaïs , Hunter Eric J. 
Published: January 2025

Purpose:
This scoping review aimed to explore the use of volitional voice tasks in assessing swallowing-related outcomes and to evaluate their therapeutic impact on swallowing disorders, including their effects on swallowing biomechanics.

Method:
This scoping review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews guidelines. A literature search was performed across multiple databases (PubMed, Web of Science, and Scopus), and additional records were identified through manual searches. After screening and eligibility assessment, 36 studies were included for data extraction and analysis. The Effective Public Health Practice Project Quality Assessment Tool for Quantitative Studies was employed to evaluate the quality of the included studies.

Results:
The review identified various volitional voice tasks, such as maximum phonation time and pitch glides, as potential assessment tools for predicting swallowing-related outcomes. Additionally, voice tasks targeting pitch modulation, increased vocal loudness, and squeezed voice quality showed promising therapeutic benefits for swallowing disorders across different populations, especially individuals with neurological conditions and head and neck cancer.

Conclusions:
While methodological limitations were found in current literature, volitional voice tasks demonstrate potential as complementary tools for assessing and treating swallowing disorders, leveraging their interconnected neurological and biomechanical mechanisms underlying functions. Further research with more robust methodologies is needed to establish the efficacy of these integrated interventions, facilitate their translation into clinical practice, and test new possibilities.
Dual-Task Interference in the Assessment of Listening Effort Before and After Cochlear Implantation in Adults: A Longitudinal Study
Authors: Ceuleers Dorien , Dhooge Ingeborg , Baudonck Nele , Swinnen Freya , Kestens Katrien , Keppler Hannah 
Published: January 2025

Purpose:
This study aimed to assess the magnitude and direction of dual-task interference in a listening effort dual-task paradigm in individuals with severe-to-profound hearing loss before and in the short- and long-term after cochlear implantation.

Design:
The study sample consisted of 26 adult candidates for cochlear implantation with severe-to-profound hearing loss. The dual-task paradigm consisted of a primary speech understanding task, conducted in a quiet condition, and a favorable and unfavorable noise condition on the one hand and a secondary visual memory task on the other hand. The dual-task effect for both tasks and the derived patterns of dual-task interference were determined. Participants were evaluated at four test moments: before cochlear implantation and at 3 months, 6 months, and 12 months after implantation.

Results:
Across all listening conditions, a shift was observed from patterns of dual-task interference with worse and stable scores for the primary speech understanding task in the dual-task condition compared to the baseline condition before implantation, toward patterns in which stable or better scores were obtained, respectively, for the primary task in the dual-task condition after implantation. This indicates that more attention could be allocated to the primary speech understanding task during the dual-task condition after implantation, implying a decreased listening effort.

Conclusions:
A decreased listening effort was found after cochlear implantation. This study provides additional insights into the evolution of dual-task interference after cochlear implantation. It highlights the importance of interpreting both the primary and secondary tasks using a dual-task paradigm in the assessment of listening effort.
Point‐PC: Point cloud completion guided by prior knowledge via causal inference
Authors: Gao Xuesong , Jiao Chuanqi , Chen Ruidong , Wang Weijie , Nie Weizhi 
Published: January 2025

AbstractThe goal of point cloud completion is to reconstruct raw scanned point clouds acquired from incomplete observations due to occlusion and restricted viewpoints. Numerous methods use a partial‐to‐complete framework, directly predicting missing components via global characteristics extracted from incomplete inputs. However, this makes detail recovery challenging, as global characteristics fail to provide complete missing component specifics. A new point cloud completion method named Point‐PC is proposed. A memory network and a causal inference model are separately designed to introduce shape priors and select absent shape information as supplementary geometric factors for aiding completion. Concretely, a memory mechanism is proposed to store complete shape features and their associated shapes in a key‐value format. The authors design a pre‐training strategy that uses contrastive learning to map incomplete shape features into the complete shape feature domain, enabling retrieval of analogous shapes from incomplete inputs. In addition, the authors employ backdoor adjustment to eliminate confounders, which are shape prior components sharing identical semantic structures with incomplete inputs. Experiments conducted on three datasets show that our method achieves superior performance compared to state‐of‐the‐art approaches. The code for Point‐PC can be accessed by https://github.com/bizbard/Point‐PC.git.