bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcomes for Adaptive Mentalization Based Integrative Treatment informed care for adolescents using a deployment‐based approach
Authors: Fuggle Peter , Fairbairn James , Fonagy Peter 
Published: September 2023

AbstractPurposeAdolescent mentalisation‐based integrative therapy (AMBIT) is a whole‐systems approach designed to enhance the effectiveness and coordination of care for clients experiencing severe and pervasive difficulties in social and health care settings, who have not responded to traditional clinical approaches. AMBIT is a team‐based manualised method that primarily aims to bolster mental state understanding and discourse focused on the client within and between teams. Over 300 teams worldwide have been trained in and adhere to AMBIT principles.MethodIn this paper, we review and summarise the outcomes reported by eight AMBIT‐informed teams that have published their findings with young people. Each report is discussed, and limitations of the data provided are identified.ResultsA synthesis of the findings across the studies suggests a generally positive impact of teams informed by AMBIT with moderate to large effect sizes on reducing symptoms and improving functionality.ConclusionsThis study suggests that AMBIT may be a promising approach for young people with multiple problems but further research is needed to identify the active mechanisms of change in complex helping systems.
Spatial aggregation patterns in four mistletoe species: ecological and environmental determinants
Authors: Ojeda S. , Arancibia M. , Gómez F. , Sepúlveda I. B. , Orellana J. I. , Fontúrbel F. E. 
Published: September 2023

Abstract
Plant spatial distribution is an important topic in ecology as it determines species coexistence and biodiversity dynamics. Usually, plants show clustered distributions in nature. Mistletoes are a good example of aggregated distributions, as they form dense aggregations due to several factors (availability of competent hosts, seed dispersal vectors, microclimate conditions).
We analysed four native mistletoe species with divergent life histories and host ranges: Desmaria mutabilis and Tristerix corymbosus from the temperate rainforests of southern Chile; and Tristerix aphyllus and Tristerix verticillatus from the northern semi‐desert zone. While T. corymbosus and T. verticillatus have a wide host range, T. aphyllus and D. mutabilis are specialists that can parasitize only a few plant species. We hypothesized that specialized species would be more aggregated due to ecological and environmental restrictions. We used heterogeneous Poisson models to quantify spatial aggregation.
Three of the four mistletoe species were spatially clustered at both environments, with aggregation being stronger in the temperate rainforest of southern Chile and particularly in the host‐specialist species. Our results suggest that environmental constraints are more important than ecological constraints (host range) in shaping mistletoe spatial structure.
Mistletoe aggregated spatial distribution depends primarily on the environment that they inhabit, which conditions host spatial availability, and arrangement.
Identification and adaptive evolution analysis of glutaredoxin genes in Populus spp.
Authors: Wang S. , Dong Y. , Gu L. , Chen X. , Zhang C. , Long L. , Wang J. , Yang M. 
Published: September 2023

Abstract
Glutaredoxin (GRX) is a class of small redox proteins widely involved in cellular redox homeostasis and the regulation of various cellular processes. The role of GRX gene in the differentiation of Populus spp. is rarely reported.
We compared the similarities and differences of GRX genes among four sections of poplar using bioinformatics, corrected the annotations of some GRX genes, and focused on analysing their transcript profiling and adaptive evolution in Populus spp.
A total of 219 GRX genes were identified in four sections of poplar, among which annotations for 13 genes were corrected. Differences in GRX genes were found between sect. Turanga, represented by P. euphratica, and other poplar sections. Most notably, P. euphratica had the smallest number of duplication events for GRX genes (n = 9) and no tandem duplications, whereas there were >25 duplication events for all other poplars. Furthermore, we detected 18 pairs of GRX genes under positive selection pressure in various sections of poplar, and identified two groups of GRX genes in the Salicaceae that potentially underwent positive selection. Expression profiling results showed that the PtrGRX34 and its orthologous genes were upregulated under stress treatments.
In summary, the GRX gene family underwent expansion during poplar differentiation, and some genes underwent rapid evolution during this process, which may be beneficial for Populus spp. to adapt to environmental changes. This study may provide more insights into the molecular mechanisms of Populus spp. adaptation to environmental changes and the adaptive evolution of GRX genes.
Asthma in developing countries in the Asia‐Pacific Region (APR)
Authors: Yasaratne Duminda , Idrose N. Sabrina , Dharmage Shyamali C. 
Published: September 2023

AbstractThere is growing interest in the epidemiology of asthma in developing countries, especially in the Asia‐Pacific Region (APR). A number of reviews have been published in this field, but a comprehensive synthesis of overall data has not been reported. Here, we summarized the burden, risk factors and challenges of asthma management in developing countries with a specific emphasis on the APR by consolidating evidence from both systematic and narrative reviews published up until February 2023. We found that although asthma prevalence in low and low‐middle‐income countries (LMICs) is known to be generally lower compared to high‐income countries, the burden is substantially greater. Studies conducted in APR LMIC have reported a range of risk factors, including pre‐ and post‐natal factors, environmental considerations, lifestyle measures, individual features and genetics. The low and inequitable distribution of quality preventive and curative health care, a lack of advanced diagnostic measures, non‐availability and non‐affordability of novel therapeutics, cultural beliefs and practices, and diverse disease phenotypes make it challenging to achieve optimal asthma control in the region. Hence, we call for the development of a region‐specific blueprint for action to mitigate this challenging situation, to help reduce the burden of asthma in APR LMIC.
Oral hygiene practice and home‐care challenges in children with autism spectrum disorder in San Francisco: Cross‐sectional study
Authors: Nagda Rahul , Le Thuan , Lin Brent , Tanbonliong Thomas 
Published: September 2023

AbstractIntroductionAutism spectrum disorder (ASD) is characterized by challenges in social interaction, communication, and stereotypical patterns of behavior. They are more likely to have tooth brushing issues and need help with routine dental care. Children with ASD could face difficulty in accessing dental care as the parents have a challenging task in finding a dentist that would treat the child with special needs. As a result, dental care is often neglected. The aims of this study are to evaluate oral hygiene practices and challenges in children with ASD and compare it with typically developing peers (TDP).MethodThe sample size of the study was 60. Parents of children aged 3–10 were recruited and divided in 2 groups with 30 in each group. Age and gender of children were matched. ASD group included children with medical diagnosis of ASD only and no other co‐morbidities. Children in TDP group had no diagnosis of ASD, and reported no disability, or any other diagnosis. Parents were asked to complete the questionnaire which asked about their demographic information, child's oral care regimens, and their knowledge about dental care. Chi‐squared tests and t‐test were used for statistical analysis. A p‐value of .05 was used for determining statistical significance.ResultsMany children with ASD (47%) had to travel more than 20 miles for their dental home compared to 13% with TDP (p < .05). Only 60% of children with ASD brushed twice a day with 70% of parents reporting that it is difficult to brush their child's teeth compared to TDP with 93% of children brush twice daily (p < .05). Significantly a smaller number of children with ASD (47%) allowed their parent to brush their teeth as compared to 93% of TSD children (p < .001).ConclusionParents of children with autism spectrum disorder travel longer distance to find a dental home and face more difficulty in maintaining their child's daily oral hygiene.
The relationship between self‐deception and other‐deception
Authors: Wehofsits Anna 
Published: September 2023

AbstractUnlike the question of whether self‐deception can be understood on the model of other‐deception, the relationship between the two phenomena at the level of practice is hardly ever explored. Other‐deception can support self‐deception and vice versa. Self‐deception often affects not only the beliefs and behavior of the self‐deceiving person but also the beliefs and behavior of others who may become accomplices of self‐deception. As I will show, however, it is difficult to describe this supportive relationship between self‐deception and the deception of others without conceptual contradiction. While “deflationary” approaches offer a convincing way to avoid the so‐called paradoxes of self‐deception, they do not resolve the conceptual tensions that arise here. I conclude by outlining a solution.
Glued on for the grandkids: The gendered politics of care in the global environmental movement
Authors: Gardner Peter , Williams Susannah , Macdonald Andrew 
Published: September 2023

AbstractThis paper explores the gendered politics of care in global environmental activism. Drawing on interviews with 96 Extinction Rebellion activists worldwide and a close analysis of 10 older women within this dataset, we contend that ‘care’ both replicated and contradicted the patriarchal order. Older women in Extinction Rebellion have often been relied upon to take on much of the caring labour involved in the maintenance of the movement at local and national levels. However, care also involved these women undertaking powerful—and empowering—forms of political action, often grounded in their knowledge and experience of organising social justice movements over decades. In contrast to prior research in the area, we found that women's participation in leadership roles within the movement against climate change appears to have increased over time. Using Sara Ahmed's (2004) concept of affective economies, we argue that the emotion of care came to be accumulated and attached to older women within Extinction Rebellion, producing inequalities in expectations for who would ‘care for the movement’ while also opening up opportunities for empowerment.
Do gender and educational level predict vaccination? The mediating role of attitudes towards vaccines and fear of COVID‐19
Authors: Espejo Begoña , Checa Irene 
Published: September 2023

AbstractGiven that the relationship between educational level, gender, and the fact of getting vaccinated does not seem to be clear, the aim of this research has been to verify if the beliefs towards vaccines and the fear of COVID‐19 are mediating this relationship in a general Spanish sample of 761 participants. A logistic regression with latent variables was estimated using Mplus. The results showed that there is no direct effect of gender or educational level on vaccination but both, fear of COVID‐19 and attitudes towards vaccines, act as mediators. Specifically, people with university studies show higher scores in trust of vaccine benefits, which in turn is a good predictor of getting vaccinated or not. So that having university studies and confidence in vaccines better predict getting vaccinated. Furthermore, being a woman with high levels of fear of COVID‐19, as well as having up to higher education and showing high levels of fear of COVID‐19, better predict getting vaccinated. However, this is a non‐probabilistic sample, and similar studies should be carried out with a representative sample of the Spanish population and of another countries, in which the rate of people vaccinated against other viruses is declining. This study reports the importance of a model including mediating variables when analyzing the influence of sociodemographic variables on deciding to get vaccinated or not, because this kind of model allow the detection of specific groups with low probability of vaccination, which would allow the design of specific strategies.
Therapeutic trial of fluvastatin in a cell line xenograft model of canine mammary gland cancer
Authors: Atmane Mohamed Issam , Vigneau Anne‐Laurence , Beaudry Francis , Rico Charlène , Boerboom Derek , Paquet Marilène 
Published: September 2023

AbstractThe Hippo signalling pathway is involved in breast cancer and canine mammary tumour (CMT). This study sought to evaluate the efficacy of fluvastatin on the Hippo pathway and its main effectors, YAP and TAZ, in vivo in a murine CMT cell line xenograft model. On treatment day 1, mice were divided into four groups: vehicle, fluvastatin, doxorubicin or a combination therapy. Tumour volumes were monitored with callipers and tissues harvested on day 28th of treatment. Histopathological examination of tumour tissues and major organs was performed as well as tumour evaluation of necrosis, apoptosis, cellular proliferation, expression of YAP, TAZ and the mRNA levels of four of their target genes (CTGF, CYR61, ANKRD1 and RHAMM2). Results showed a statistically significant variation in tumour volumes only for the combination therapy and final tumour weight only for the doxorubicin group compared to control. There was no significant difference in tumour necrosis, expression of CC3, ki‐67, YAP and TAZ measured by immunohistochemistry and in the mRNA levels of the target genes. Unexpectedly, lung metastases were found in the control group (9) and not in the fluvastatin treated group (7). In addition, mass spectrometry‐based quantification of fluvastatin reveals concentrations comparable to levels reported to exert therapeutic effects. This study shows that fluvastatin tumours concentration reached therapeutic levels without having an effect on the hippo pathway or various tumour parameters. Interestingly, only the control group had lung metastases. This study is the first to explore the repurposing of statins for cancer treatment in veterinary medicine.
Experiences with remote communication in adult intensive care units during the COVID-19 pandemic: a systematic review protocol
Authors: Cardoso Ana Filipa , Pires Miguel Grilo , Cioga Elisabete , Abalroado Inês , Santos Diana , Duque Filipa Margarida , Loureiro Ricardo , Felizardo Helena , Fernandes António Manuel , Silva Rosa , Ventura Filipa , Santana Elaine , Cardoso Daniela , Loureiro Luís 
Published: September 2023

Objective:
The objective of this review is to explore the lived experiences of critically ill adults, their families, or health care professionals with remote communication in intensive care units (ICUs) during the COVID-19 pandemic.

Introduction:
Family visiting restrictions in ICUs during the COVID-19 pandemic imposed significant challenges to communication between critically ill adults, their families, and the health care team. Evidence shows that several communication strategies were developed and implemented in ICUs during the COVID-19 pandemic to promote family engagement; however, the experiences of critically ill adults, their families, and health care professionals with these strategies are scattered across primary qualitative studies.

Inclusion criteria:
This review will consider qualitative studies that include critically ill adults, their families, or health care professionals, focusing on their experiences with remote communication strategies in ICUs during the COVID-19 pandemic.

Methods:
This review will be conducted in accordance with JBI methodology. The search strategy will aim to locate both published and unpublished qualitative studies in English, Spanish, and Portuguese. Studies published after January 2020 will be included. Study selection, critical appraisal, and data extraction will be performed independently by 2 reviewers. Data will be presented in narrative format and synthesized using the JBI meta-aggregation process. A ConQual Summary of Findings will be presented.

Review registration:
PROSPERO CRD42022383603
Gut check: Unveiling the influence of acute exercise on the gut microbiota
Authors: Grosicki Gregory J. , Langan Sean P. , Bagley James R. , Galpin Andrew J. , Garner Dan , Hampton‐Marcell Jarrad T. , Allen Jacob M. , Robinson Austin T. 
Published: September 2023

AbstractThe human gastrointestinal microbiota and its unique metabolites regulate a diverse array of physiological processes with substantial implications for human health and performance. Chronic exercise training positively modulates the gut microbiota and its metabolic output. The benefits of chronic exercise for the gut microbiota may be influenced by acute changes in microbial community structure and function that follow a single exercise bout (i.e., acute exercise). Thus, an improved understanding of changes in the gut microbiota that occur with acute exercise could aid in the development of evidence‐based exercise training strategies to target the gut microbiota more effectively. In this review, we provide a comprehensive summary of the existing literature on the acute and very short‐term (<3 weeks) exercise responses of the gut microbiota and faecal metabolites in humans. We conclude by highlighting gaps in the literature and providing recommendations for future research in this area.
Shear-reversible clusters of HIV-1 in solution: stabilized by antibodies, dispersed by mucin
Authors: Ogundiran Ayobami I. , Chang Tzu-Lan , Ivanov Andrey , Kumari Namita , Nekhai Sergei , Chandran Preethi L. 
Published: September 2023

ABSTRACT
HIV-1 has eluded vaccine therapy for the past 40 years. The virus mutates rapidly and is protected by a shifting glycan shield of mannose sugars, which has hindered the broad neutralization of the virus by antibodies (Abs). Studies have shown that mannose residues are self-adhesive, but it is not known if these adhesions drive HIV-1 to aggregate in solution, further complicating Ab neutralization. The behavior of HIV-1 in culture media was monitored using Dynamic Light Scattering and complementary atomic force microscopy (AFM) imaging in the presence of anti-gp120 Abs, lectins, mannosidase, and mucin. After accounting for the serum contribution from the culture media, HIV-1 was found to be diffusing in solution in 400–700 nm clusters. These clusters could be sheared into single virus particles by filtration, but the dispersed particles clustered back within a short time frame. Sample preparation prior to AFM and transmission electron microscopy (TEM) imaging appears to disperse clusters, but the clusters become visible in AFM when they are stabilized by Abs in solution. The clustered form of the virus appears to restrict access of Abs, lectins, and glycosidases to surfaces within the cluster. Mannosidase treatment following virus dispersion by filtration prevented clustering, suggesting that the mannose glycan shield is involved in cluster formation. Dispersed HIV-1 particles that were bound by Abs did not re-cluster back. Free mucin molecules (porcine gastric mucin) effectively dispersed HIV-1 clusters, even those stabilized by Abs. HIV-1-loaded mucin dried on the AFM surface with a fern-like fractal pattern, similar to that seen clinically in cervical mucin during the more penetrable ovulation stage.

IMPORTANCE
The phenomenon of reversible clustering is expected to further nuance HIV immune stealth because virus surfaces can escape interaction with antibodies (Abs) by hiding temporarily within clusters. It is well known that mucin reduces HIV virulence, and the current perspective is that mucin aggregates HIV-1 to reduce infections. Our findings, however, suggest that mucin is dispersing HIV clusters. The study proposes a new paradigm for how HIV-1 may broadly evade Ab recognition with reversible clustering and why mucin effectively neutralizes HIV-1.
Multi-integrated approach for unraveling small open reading frames potentially associated with secondary metabolism in
Streptomyces
Authors: Fan Si-Min , Li Ze-Qi , Zhang Shi-Zhe , Chen Liang-Yu , Wei Xi-Ying , Liang Jian , Zhao Xin-Qing , Su Chun 
Published: September 2023

ABSTRACT

Small open reading frames (smORFs) are widely distributed in various living organisms. However, their functions remain largely unexplored. In addition, annotation and detection of smORFs are limited using existing methods and hindered by their specific properties. In this study, we systematically investigated smORFs and smORF-encoded peptides (SEPs) in
Streptomyces
, which are well-known bacterial producers of diverse bioactive secondary metabolites. We established a peptidogenomic workflow based on multi-integrated comprehensive database search and database-independent
de novo
sequencing to identify smORFs in
Streptomyces xinghaiensis
NRRL B-24674
T
(S187). In addition, we described SEPome related to the secondary metabolism, which include 68 novel SEPs and 79 common smORFs with
Streptomyces coelicolor
A3 (2). Functional analysis of universal smORFs revealed enrichment in biosynthetic processes, stress response, ribosomes, and nucleic acid binding. Meanwhile, 5 Cryptic smORF-encoded Peptides (CSEPs) distributed in non-annotated regions of the genome, and non-coding RNAs could encode for CSEPs. A total of 66 new RNAs, including 32 non-coding RNAs (ncRNAs) were revealed, and 4 ncRNA-encoded peptides were identified. Furthermore, an investigation of carbon metabolism showed that NagE functions in spore formation and secondary metabolism in
Streptomyces
. Particularly, NagE was observed to function in the biosynthesis of anti-complement agents in
S. xinghaiensis,
suggesting a novel role of the phosphoenolpyruvate phosphotransferase system in microbial secondary metabolism. We thus provide an effective strategy for analyzing public data sets of model strains to identify smORFs for non-model species. The ncRNAs and SEPs present rich sources for engineering streptomycetes to produce bioactive compounds.

Importance
Due to their small size and special chemical features, small open reading frame (smORF)-encoding peptides (SEPs) are often neglected. However, they may play critical roles in regulating gene expression, enzyme activity, and metabolite production. Studies on bacterial microproteins have mainly focused on pathogenic bacteria, which are importance to systematically investigate SEPs in streptomycetes and are rich sources of bioactive secondary metabolites. Our study is the first to perform a global identification of smORFs in streptomycetes. We established a peptidogenomic workflow for non-model microbial strains and identified multiple novel smORFs that are potentially linked to secondary metabolism in streptomycetes. Our multi-integrated approach in this study is meaningful to improve the quality and quantity of the detected smORFs. Ultimately, the workflow we established could be extended to other organisms and would benefit the genome mining of microproteins with critical functions for regulation and engineering useful microorganisms.