bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breaking-down tumoral physical barrier by “on-site” unwrapping metal-polyphenol-packaged hyaluronidase for optimizing photothermal/photodynamic therapy-induced immune response
Authors: Yin Dengke , Sun Quanwei , Li Yunlong , Shen Wei , Shang Wencui , Xu Yujing , Yang Jinming , Chen Jie , Gao Wenheng , Wu Qinghua , Xu Fan , Yang Ye 
Published: September 2023

Abstract
The therapy of solid tumors is often hindered by the compact and rigid tumoral extracellular matrix (TECM). Precise reduction of TECM by hyaluronidase (HAase) in combination with nanotechnology is promising for solid tumor therapeutics, yet remains an enormous challenge. Inspired by the treatment of iron poisoning, here we propose an “on-site” unwrapping strategy of metal-polyphenol-packaged HAase (named PPFH) by sequentially injecting PPFH and a clinically used iron-chelator deferoxamine (DFO). The in situ dynamic disassembly of PPFH could be triggered by the intravenously injected DFO, resulting in the release, reactivation and deep penetration of encapsulated HAase inside tumors. Such a cost-effective HAase delivery strategy memorably improved the subsequent photothermal and photodynamic therapy (PTT/PDT)-induced intratumoral infiltration of CD8+ T cells and the cross-talk between tumor and tumor-draining lymph nodes (TDLN), thereby decreasing the immunosuppression and optimizing tumoricidal immune response that can efficiently protect mice from tumor growth, metastasis and recurrence in multiple mouse cancer models. Overall, this work presents a proof-of-concept of the dynamic disassembly of metal-polyphenol nanoparticles for extracellular drug delivery as well as the modulation of TECM and immunosuppressive TME.
Optimization of electro-Fenton process variables in terms of dye removal and energy consumption with response surface methodology
Authors: Karabacakoğlu Belgin , Karaduman Serhat 
Published: September 2023

Abstract
Waste water containing reactive textile dyes constitutes an important environmental problem as they are permanent and dangerous. The Electro-Fenton (EF) method is promising as an effective technique for the degradation of organic materials such as dyestuffs. In this study, the optimization of effective variables in the removal of reactive yellow 145 azo textile dye by the EF using response surface methodology (RSM) was investigated. Central composite design (CCD) was used to study the combined effects of key parameters such as voltage (2.5–12.5 V), H2O2 addition (0.1–1.3 mL), pH (1.75–4.75), electrode spacing (0.25–3.25 cm), and treatment time (25–85 min). The optimum values of the variables to ensure the highest dye removal efficiency (approx. 93%) with the lowest energy consumption (approx. 3.4 Wh/L) are approximately 9.4 V voltage, pH 3.7, 2.5 cm electrode range, 1 mL H2O2 addition, and a 40-minute treatment time. ANOVA analysis of the predicted quadratic polynomial model showed a regression coefficient value of approximately 0.97 for both objective functions; this highlights the applicability of the model for navigating the design space. Simultaneous optimization of process parameters using RSM was achieved with a low number of experiments.
Effects of Various Remineralization Agents on Surface and Subsurface Enamel Microhardness Following Demineralization
Authors: Kes Gamze , Atalay Cansu , Baseren Meserret 
Published: September 2023

Abstract
Objectives: The effects of different remineralization agents applied on enamel samples were evaluated by using surface and subsurface microhardness analysis.
Materials&Methods: Sixty bovine incisor enamel samples were fabricated. After the initial microhardness values were measured, enamel samples are divided into 3 equal parts, and assigned as the treatment area (T), the adjacent area (A) and the control area (C) (covered with an acid-resistant varnish). Samples were subjected to pH cycle for 5-days, and then microhardness measurements were repeated. The specimens were randomly divided into 5 groups according to the fluoride-releasing materials applied, as follows: Group 1: BioMin F; Group 2: Colgate Triple Effect; Group 3: FCP COMPLEX; Group 4: Fluor Protector; Group 5: Artificial saliva (control). Following the applications, surface and subsurface measurements were performed. The data were analyzed by one-way analysis of variance (ANOVA) and post-hoc Tukey test.
Results: BioMin F toothpaste provided the greatest increase in surface microhardness, followed by FCP COMPLEX, Colgate Triple Effect and Fluor Protector, respectively. Among the materials tested, BioMin F and Colgate toothpastes exhibited the highest increase in subsurface microhardness values, with FCP COMPLEX solution following closely in second place.
Conclusions: BioMin F showed the best results in the surface microhardness assessment, while both toothpastes (BioMin F and Colgate) showed similarly positive results in the subsurface microhardness assessment.
Clinical relevance: Remineralizing agents used in addition to fluoride may increase the effectiveness in remineralization of initial carious lesions.
A protocatechuic acid-based deep eutectic solvent: a novel and versatile catalyst for the green synthesis of pyranopyrimidines
Authors: Monem Arezo , Habibi Davood , Goudarzi Hadis 
Published: September 2023

Abstract
A novel deep eutectic solvent (MTPPBr-PCAT-DES) was prepared from a one-to-one mole ratio of methyltriphenylphosphonium bromide (MTPPBr) and 3,4-dihydroxybenzoic acid (PCAT=protocatechuic acid) and characterized by various techniques such as FT-IR, TGA/DTA, densitometer, eutectic point, and 1H NMR. Then, it was used as a novel and capable catalyst for the synthesis of pyranopyrimidines from the multicomponent condensation reaction of barbituric acid, 4-hydroxycoumarin, and aromatic aldehydes in mild conditions, short reaction times, and high yields.
Mannich-Type Modifications Of (-)-Cannabidiol And (-)-Cannabigerol Leading To New, Bioactive Derivatives
Authors: Lőrincz Eszter Boglárka , Tóth Gergely , Spolárics Júlia , Herczeg Mihály , Hodek Jan , Zupko István , Minorics Renáta , Ádám Dorottya , Oláh Attila , Zouboulis Christos C. , Weber Jan , Nagy Lajos , Ostorházi Eszter , Bácskay Ildikó , Borbás Anikó , Herczegh Pál , Bereczki Ilona 
Published: September 2023

Abstract

(‑)-Cannabidiol (CBD) and (‑)-cannabigerol (CBG) are two major non-psychotropic phytocannabinoids that have many beneficial biological properties. However, due to their low water solubility and prominent first-pass metabolism, their oral bioavailability is moderate, which is unfavorable for medicinal use. Therefore, there is a great need for appropriate chemical modifications to improve their physicochemical and biological properties. In this study, Mannich reaction was used for the synthetic modification of CBD and CBG for the first time, and thus fifteen new cannabinoid derivatives containing one or two tertiary amino groups were prepared. Thereafter the antiviral, antiproliferative and antibacterial properties of the derivatives and their effects on certain skin cells were investigated. Some modified CBD derivatives showed remarkable antiviral activity against SARS-CoV-2 without cytotoxic effect, while synthetic modifications on CBG resulted in a significant increase in antiproliferative activity in some cases compared to the parent compound.
Experimental insights into electrocatalytic [Cp*Rh(bpy)Cl]+ mediated NADH regeneration
Authors: Meyer Jonas , Romero Manuela , Thöming Jorg , Baune Michael , Reimer Nicholas , Dringen Ralf , Bösing Ingmar 
Published: September 2023

Abstract
NADH plays a crucial role in many enzymatically catalysed reactions. Due to the high costs of NADH a regeneration mechanism of this cofactor can enlarge the applications of enzymatic reactions dramatically. This paper gives a thorough system analysis of the mediated electrochemical regeneration of active NADH using cyclic voltammograms and potentiostatic measurements with varying pH, electr ode potential, and electrolyte solution, highlighting the system’s limiting conditions, elucidat ing optimal working parameters for the electrochemical reduction of NAD+ , and bringing new insight on the oxidation of inactive reduction products. Using [Cp*Rh(bpy)Cl]+ as an electron mediator dramatically incre ases the percentage of enzymatically active electrochemically reduced NADH from 15 % (direct) to 99 % (mediated) with an Faradaic efficiency of up to 86 %. Furthermore, investigations of the catalytic mechanisms of [Cp*Rh(bpy)Cl]+ clarifies the necessary conditions for its functioning and questions the proposed reaction mechanism by two-step reduction where first the mediator is reduced and then brought in contact with NAD+.
A Case Report of Warm Autoimmune Haemolytic Anaemia
Authors: Raulji Jay , Thakore Dhruvin 
Published: September 2023

Abstract
Hemolytic anemia is a hematologic disorder characterized by the premature destruction of red blood cells (RBCs). This can lead to a shortage of RBCs, which can cause a variety of symptoms, including fatigue, shortness of breath, and jaundice.
The destruction of RBCs can occur in two main ways: intravascular hemolysis and extravascular hemolysis. In intravascular hemolysis, the RBCs are destroyed within the bloodstream. This can be caused by factors such as immune-mediated hemolysis, mechanical trauma, or infections. In extravascular hemolysis, the RBCs are destroyed by the spleen and other organs of the reticuloendothelial system. This can be caused by factors such as hereditary defects in RBCs, infections, and medications.
The diagnosis of hemolytic anemia is made based on a clinical evaluation and a variety of laboratory tests. The clinical evaluation should focus on identifying the symptoms and signs of hemolytic anemia, as well as any underlying conditions that may be causing the hemolysis. The laboratory tests that are typically performed include a complete blood count (CBC), a direct antiglobulin test (DAT), a haptoglobin test, and a bilirubin test.
The treatment of hemolytic anemia depends on the underlying cause. In some cases, no treatment is necessary. In other cases, treatment may include medications, blood transfusions, or surgery.
The prognosis for hemolytic anemia varies depending on the underlying cause. Some types of hemolytic anemia are chronic and lifelong, while others can be cured.
Daily Breath-Based Mindfulness Exercises Improve Primary School Children’s Performance in Arithmetic: A Randomized Controlled Trial
Authors: Hondrich Finja , von Salisch Maria , Voltmer Katharina 
Published: September 2023

Abstract
Mindfulness-based interventions (MBIs) have been shown to improve children’s academic achievements. Because MBIs include different exercises (with different effects), the teacher-led Breathing Break Intervention (BBI) with daily breath-based mindfulness practices was developed and evaluated in terms of its effects on children’s performance in mathematics. In a randomized controlled trial, N = 140 third and fourth graders (49% female) either received BBI (IG, n = 81) or participated in an active control group (ACG, n = 59). Students took a standardized arithmetic test and teachers rated their mathematics performance before (T1) and after (T2) the ten weeks of BBI, and in a follow-up five months later (T3).
A mixed multilevel model with a quadratic term of time indicated a significant interaction effect between group and time on the arithmetic test after controlling for working memory updating and socioeconomic status. IG children differed significantly from ACG children in their trajectory of arithmetic performance and outperformed them at T3. A multilevel ordinal logistic regression of teachers’ ratings of students’ mathematics performance revealed no significant differences between IG and ACG. Daily breathing exercises in primary school classrooms seem to be effective in enhancing children's performance in arithmetic.
Preregistration: The study was preregistered at aspredicted.org (#44925)
A discrete collocation technique using the thin plate splines for solving a certain class of integro-differential equations arising in biology models
Authors: Assari Pouria , Asadi-Mehregan Fatemeh , Dehghan Mehdi 
Published: September 2023

Abstract
The primary motivation of the current article is to study an approximate algorithm to solve a type of Volterra integro-differential equations involving nonlinear terms. These integro-differential equations have been utilized to simulate several realistic modelling emanated in biological sciences, for example, the growth model of the toxins' cumulative effects on a population residing in a closed system. Thin plate splines (TPSs), known as a subclass of radial basis functions (RBFs) independent of the shape parameter, create an efficient scheme to interpolate a function. Due to this desirable property, we use the discrete collocation approach with the TPS basis for estimating the solution of mentioned integro-differential equations. Furthermore, the Gauss-Legendre integration formula is utilized to calculate the integrals emerging in the scheme. Remarkably, the algorithm of the offered approach can be comfortably performed on a PC with typical specifications derived from the simplicity of using TPSs. The researchers also investigate the convergence of the presented scheme. Some test examples including Volterra integro-differential equations are considered to make sure the veracity of the proposed scheme and the validity of the theoretical error analysis.
MSC (2020): 45D05; 45J05; 92-08;92D25;
Remote Control of Bovine Catalase Activity with Specific Frequencies of Human and Bovine Catalase with and without bound NADP+
Authors: d'Oultremont Thibaud Etienne 
Published: September 2023

Abstract
Remote control enzyme technology is widely used today through resonance. In this study, we showed that the use of frequencies of the catalase enzyme itself to increase enzymatic rate is successful not only in test tubes but also remotely. The present study also suggests that, under optimal temperature, the use of bovine catalase frequency (the specific frequency of that enzyme) has a superior rate promoting vibration than the human catalase frequency, and so increases very significantly the chemical rate of bovine catalase (about 120% at 40ºC). It also suggests that bovine catalase subjected to bovine and human frequencies with catalase bound NADP+ experienced more resonance weight towards NADP+ and so were more slowly reduced back to catalase bound NADPH, increasing compound II formation rate, and slowing down the catalase activity rate.
Costs and Outcomes Associated with the Administration of Intravenous Acetaminophen in Neonates after Esophageal Atresia and Tracheoesophageal Fistula Repair
Authors: Phillipi Michael , Song Ashley , Yieh Leah , Gong Cynthia 
Published: September 2023

Abstract

Objective: The primary objective was to examine the outcomes and costs associated with the use of intravenous acetaminophen (IV APAP) in combination with opioids versus opioids alone as a pain management strategy after neonatal esophageal atresia (EA) and tracheoesophageal fistula (TEF) repair.
Study Design: Data from the Pediatric Health Information System was used to examine 1 137 hospitalizations for EA/TEF repair from 2015 to 2018. Neonates administered opioids only, or IV APAP in combination with opioids as pain management were included.
Result: Neonates receiving IV APAP experienced a longer median length-of-stay (LOS), but a significantly lower mortality rate, a decreased mean daily cost, and reduced opioid use compared to neonates given only opioids. There were no significant differences in pharmacy and total costs.
Conclusion: Our findings suggest the use of IV APAP alongside opioids in EA/TEF repair is associated with reduced mortality and opioid use, as well as longer LOS.
Holding in the stream – convergent evolution of suckermouth structures in Loricariidae (Siluriformes)
Authors: Krings Wencke , Konn-Vetterlein Daniel , Gorb Stanislav N. 
Published: September 2023

Abstract
Suckermouth armoured catfish (Loricariidae) are a highly specious and diverse freshwater fish family, which bear upper and lower lips forming an oral disc. Its hierarchical organisation allows the attachment to various natural surfaces. The discs can possess papillae of different shapes, which are supplemented, in many taxa, by small horny projections, i.e. unculi. Although these attachment structures and their working mechanisms, which include adhesion and interlocking, are rather well investigated in some selected species, the loricariid oral disc is unfortunately understudied in majority of species, especially with regard to comparative aspects of the diverse oral structures and their relationship to the ecology of different species. In the present paper, we investigated the papilla and unculi morphologies in 69 loricariid species and undescribed taxa, which inhabit different currents and substrates. We determined four papilla types and eight unculi types differing by forms and sizes. From handling the structures and from drying artefacts we could infer some information about their material properties. This, together with their shape, enabled us to carefully propose hypotheses about mechanisms of interactions of oral disc structures with natural substrates typical for respective fish species.
A practical synthesis for the key intermediate (G) of Apixaban
Authors: Dong Wenwen , Gong Tengfei , Liu Chaoyang , Lin Jia , Jia Qiang , Chu Changhu 
Published: September 2023

Abstract
Apixaban is a highly potent, selective, and efficacious inhibitor of blood coagulation factor Xa. A practical and efficient process has been developed for the preparation of the key intermediate (G) of Apixaban. Starting from inexpensive 4-chloronitrobenzene and piperidine, an eight-step procedure for G has been developed. In this case, sodium chlorite is used twice to oxidize the piperidine cycle to the corresponding lactam under a CO2 atmosphere, resulting in the construction of two lactams. Furthermore, most of these reactions are highly efficient and practical as they occur under mild conditions. Most of the intermediates can be obtained through simple slurry or recrystallization, and column chromatography purification is not necessary.
A hybrid strength criterion for a soil medium based on tension strength and its implementation in numerical analysis
Authors: Yin Chao , Wang Shuai-wei W. , LI Jian , ZHAO Cheng-gang , Wang Wei 
Published: September 2023

Abstract
Because of inaccurate descriptions of stress‒strain relationships for the tension–shear region of the Mohr–Coulomb strength criterion, a new hybrid strength criterion called the tension–shear strength criterion was proposed and established in this study. Based on uniaxial tensile tests (using a newly developed apparatus) and triaxial shear tests, the feature points of the failure envelopes were obtained. The tension–shear strength criterion was then derived according to these points. Subsequently, to solve the discontinuous derivatives of the singularities in the yield surface, the tension–shear strength criterion was converted into its numerical form based on a new rounded method, and a constitutive integral algorithm of the tension–shear strength criterion was given. Finally, several numerical examples with theoretical and empirical solutions were adopted to validate the effectiveness and accuracy of the numerical form of the tension–shear strength criterion.
Face-bow transfer – effect of body position and the use of a novel force-controlled nasion support on the reliability of articular mounting in vivo
Authors: Ahlers M. Oliver , Roehl Jakob C. , Jakstat Holger A. 
Published: September 2023

Abstract
Objectives
This study aimed to determine whether the positioning of the patient in combination with force-controlled nasion support improves reproducibility in applying an arbitrary face-bow.
Materials and methods
22 undergraduate students participated in the study as operators and repeatedly mounted an arbitrary face-bow on a single patient. Each operator performed the face-bow transfer twice, employing two different techniques. The standard technique used the classical rigid nasion adapter on a seated patient. In contrast, the novel method utilized a force-controlled nasion adapter which limits the force to the earbuds to 3 N on a reclined patient. The sequence of techniques was randomized (crossover design). Based on the face-bow transfers, randomly chosen maxillary casts were mounted in an individual articulator. The orientation of all models in the articulator was measured with a 3D-Digitizer at three predetermined sites. The differences between the means and specific positions were calculated, the means and standard deviations were computed, and significance tests were performed (Mann-Whitney-U-Test).
Results
Statistical analysis indicated a significantly smaller variance in the vertical plane for the patient in a reclined position combined with the novel nasion support (SD novel technique 5.187 vs. standard technique 6.309; p = 0.02).
Conclusion
Using the novel force-controlled nasion support to apply the face-bow in a reclined patient position improves reproducibility.
Clinical relevance
The application method for transferring maxillary casts to individual articulators should be altered towards placing patients in a reclined position with a nasion adapter that limits the force to the earbuds for better reproducibility.
Growth and Mortality Rates of Microbial Communities Respond to Experimental Warming at Different Latitudes During the Cold Season
Authors: Olivia Madeline , Patrichka Wei-Yi Chen , Urabe Jotaro , Ho Pei-Chi , Mukhanov Vladimir , Tsai An-Yi 
Published: September 2023

Abstract
To evaluate the responses of microbial plankton communities to warming in coastal waters of the Pacific at different latitudes, three in situ microcosms were conducted in Taiwan and Japan during the winter of 2023. Flow cytometry was used for consecutive 7 days to monitor and analyze the microbial communities, including viruses, bacteria, and picophytoplankton (Synechococcus spp., Prochlorococcus spp., and picoeukaryotes). The control microcosms had the same natural water temperature as the coastal waters, while the others were subjected to a warming treatment of + 2–3°C. In addition, picoplankton (bacteria and picophytoplankton) growth, nanoflagellate grazing, and viral lysis rates were estimated by using modified dilution method on days 2 and 5 of the study. Our investigation revealed that the warming did not have a significant positive effect on the bacterial abundance in winter at all stations during the time-series incubations. However, a significant increase in viral abundance was observed following the temperature increase, indicating that viral impact can have a significant top-down effect on bacterial cells in our time series experiments. Furthermore, we also found that the degree of increase in viral abundance was related to picophytoplankton abundance which increased with warming at some stations in this study. Importantly, we also discovered a positive correlation between the increases in prokaryotic viral lysis and gross growth rates in the warming experiments. This suggests that increased warming in coastal water during cold seasons can potentially enhance the viral shunt, enabling the retention of carbon and energy within the microbial loop.