bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between family caregivers' primary care experience when they report as patients and their stress related to caregiving: A pilot cross‐sectional study
Authors: Nakayama Gen , Masumoto Shoichi , Haruta Junji , Maeno Tetsuhiro 
Published: June 2023

AbstractBackgroundFew studies have examined whether family caregivers' own primary care providers can affect caregiving‐specific well‐being, such as caregiver stress. In this pilot study, we explored whether primary care experiences when family caregivers report as patients were associated with the stress of caregiving.MethodsWe used cross‐sectional data from a survey conducted in Japan between November and December 2020. We recruited family caregivers aged 40–74 years who were caring for community‐dwelling adults with chronic conditions. We assessed primary care experience using the Japanese version of the Primary Care Assessment Tool Short Form (JPCAT‐SF) and caregiver stress using the Japanese short version of the Zarit Caregiver Burden Interview.ResultsIn total, 406 family caregivers were included in the analysis. The mean JPCAT‐SF total score was 42.1 out of 100 points. The proportion of caregivers who had higher caregiver stress was 48.8%. After adjusting for possible confounders, the JPCAT‐SF score was found to be significantly associated with caregiver stress (lower stress = 0 vs. higher stress = 1; adjusted prevalence ratio per 1 SD increase in JPCAT‐SF score = 0.89; 95% CI 0.80–0.98). Among the subscales of the JPCAT‐SF, longitudinality, and comprehensiveness (services available) were associated with caregiver stress.ConclusionsBetter primary care experiences when family caregivers reported as patients were associated with lower caregiver stress. Longitudinality, which includes focusing attention on the individual as a whole person, and comprehensiveness in the context of building provider‐patient relationships that make consultation easier when needed, were associated with lower stress.
Transcranial direct current stimulation alleviates nociceptive behavior in male rats with neuropathic pain by regulating oxidative stress and reducing neuroinflammation
Authors: Akcay Guven , Nemutlu Samur Dilara , Derin Narin 
Published: June 2023

AbstractTranscranial direct curent stimulation (tDCS) and trans‐spinal direct current stimulation (tsDCS) are promising therapies for pain that can alter the excitability of neuronal activity in cerebral cortex. The aim of the study is to investigate the therapeutic effects of direct current stimulation (DCS) over the spinal cord and cerebral cortex on oxidative stress and neuroinflammation in rats with chronic constriction injury (CCI). Male Wistar rats were randomly divided into four experimental groups: Sham, CCI, CCI + tDCS and CCI + tsDCS. The neuropathic pain model was induced by using the CCI model. Rats with neuropathy were treated with cathodal tDCS and tsDCS stimulations consisting of 0.5 mA for 30 min a day for 7 days from day 8 onwards. Locomotor activity was measured by open‐field test and nociceptive behavior was assessed by hot‐plate, tail‐flick and Randall–Selitto tests. Following the behavioral experiments, total oxidant capacity (TOC), total antioxidant capacity (TAC) and proinflammatory cytokine levels were evaluated in spinal cord and cerebral cortex tissues. The CCI model induced significant mechanical and thermal hyperalgesia. Nociceptive behaviors in rats with CCI were reversed by DCS treatment. Higher TOC and lower TAC levels were detected in the spinal cord and cerebral cortex tissues of the CCI rats compared to the control. tsDCS treatment amended oxidant/antioxidant status. Moreover, tsDCS modulated the central levels of Tumor necrosis factor‐α (TNF‐α), interleukin 1‐beta (IL‐1β), IL‐6 and IL‐18. tsDCS stimulation showed better therapeutic effect on neuropathic pain by regulating oxidant/antioxidant levels and reducing neuroinflammation. DCS, especially at spinal level, may be a promising therapeutic strategy that can be used alone or in combination with other effective treatments for alleviating neuropathic pain.
Efficacy of intra‐articular injections of hyaluronic acid in patients with glenohumeral joint osteoarthritis: A systematic review and meta‐analysis
Authors: Familiari Filippo , Ammendolia Antonio , Rupp Marco‐Christopher , Russo Raffaella , Pujia Arturo , Montalcini Tiziana , Marotta Nicola , Mercurio Michele , Galasso Olimpio , Millett Peter J. , Gasparini Giorgio , de Sire Alessandro 
Published: June 2023

AbstractSymptomatic primary glenohumeral (GH) joint osteoarthritis (OA) can be challenging to treat. Hyaluronic acid (HA) has emerged as a promising treatment for the nonsurgical management of GH‐OA. In this systematic review with meta‐analysis, we aimed to evaluate the current evidence regarding the efficacy of intra‐articular HA on pain relief in patients suffering from GH‐OA. A total of 15 studies (only randomized controlled trials providing data at the end of the intervention) were included. The relevant studies were selected based on the following PICO model: P: patients with diagnosis of shoulder OA; I: HA infiltrations as therapeutic intervention administered; C: no restriction for comparators assessed; O: pain, in terms of visual analog scale (VAS) or numeric rating scale. The risk of bias among the included studies was estimated using the PEDro scale. A total of 1023 subjects were analyzed. Comparing HA injections combined with physical therapy (PT) compared to PT alone resulted in superior scores, showing an overall effect size (ES) of 4.43 (p = 0.00006). Moreover, pooled analysis of VAS pain scores demonstrated a significant improvement in the ES of the HA in comparison with corticosteroid injections (p = 0.002). On average, we reported a PEDro score of 7.2. A total of 46.7% of studies showed probable signs of a randomization bias. The findings of this systematic review and meta‐analysis showed that IA injections of HA might be effective on pain relief with significant improvements compared to baseline and compared to corticosteroid injections in patients affected by GH‐OA.
Association between GLIM‐defined malnutrition and hospitalizations in kidney transplant candidates: A post hoc analysis of a cohort study
Authors: Dávalos‐Yerovi Vanesa , Pérez‐Sáez Maria José , Faura‐Vendrell Anna , Muns‐Cornellas Maria Dolors , Duran Xavier , Sánchez‐Rodríguez Dolores , Pascual Julio , Marco Ester ,   the FRAILMar Study Group
Published: June 2023

AbstractBackgroundMalnutrition is frequent in patients with chronic kidney disease (CKD) and has a negative impact on morbidity, mortality, and quality of life. The objective of this study was to assess the value of the Global Leadership Initiative for Malnutrition (GLIM) criteria to predict hospitalizations and mortality in candidates to kidney transplant during their first year on the waiting list.MethodsThis was a post hoc analysis of 368 patients with advanced CKD. The main study variables were malnutrition, according to the GLIM criteria; number of hospital admissions during the first year on the waiting list; and mortality at the end of follow‐up. Kaplan‐Meier survival curves and binary logistic regression were performed, adjusting for age, frailty status, handgrip strength, and Charlson Index as potential confounders.ResultsThe prevalence of malnutrition was 32.6%. Malnutrition was associated with increased risk of hospitalizations during the first year on the waiting list (odds ratio [OR] = 3.33 [95% CI = 1.34–8.26]), which persisted after adjustment for age and frailty status (adjusted OR = 3.61 [95% CI = 1.38–10.7]), age and handgrip strength (adjusted OR = 3.39 [95% CI = 1.3–8.85]), and age and Charlson Index (adjusted OR = 3.25 [95% CI = 1.29–8.13]).ConclusionMalnutrition according to the GLIM criteria was highly prevalent in patients with CKD and was associated with a threefold increased risk of hospitalizations during the first year on the waiting list; these associations remained significant after adjusting for age, frailty status, handgrip strength, and comorbidities.
Optimization of caseinolytic and coagulating activities of Solanum tuberosum rennets for cheese making
Authors: Tito Florencia R , Pepe Alfonso , Tonón Claudia V , Daleo Gustavo R , Guevara María G 
Published: June 2023

AbstractBACKGROUNDIn recent years, the rising global demand for cheese, the high cost and limited supply of calf rennet, and consumer choices have increased research into new alternatives to animal or recombinant chymosins for cheese making. Plant proteases with caseinolytic activity (CA) and milk‐clotting activity (MCA) have been proposed as alternatives for milk clotting to obtain artisanal cheeses with new organoleptic properties. They have been named vegetable rennets (vrennets). The aim of this study was to evaluate the performance of two Solanum tuberosum aspartic proteases (StAP1 and StAP3) as vrennets for cheese making and to obtain a statistical model that could predict and optimize their enzymatic activity.RESULTSTo optimize the CA and MCA activities, a response surface methodology was used. Maximum values of CA and MCA for both enzymes were found at pH 5.0 and 30–35 °C. Analysis of the degradation of casein subunits showed that it is possible to tune the specificity of both enzymes by changing the pH. At pH 6.5, the αS‐ and β‐ subunit degradation is reduced while conserving a significant MCA.CONCLUSIONThe statistical models obtained in this work showed that StAP1 and StAP3 exert CA and MCA under pH and temperature conditions compatible with those used for cheese making. The casein subunit degradation percentages obtained also allowed us to select the best conditions for the degradation of the κ‐casein subunit by StAPs. These results suggest that StAP1 and StAP3 are good candidates as vrennets for artisan cheese making. © 2023 Society of Chemical Industry.
Trainees Associated with Improved Wait Times and Patient Satisfaction in a Multidisciplinary Clinic
Authors: Yousef Andrew , Lauthen David , Ramsey Celia , Zhang Xinlian , Athas Jayna , Jenusaitis Matthew , Califano Joseph 
Published: June 2023

ObjectiveTo determine variables associated with longer wait times and decreased patient satisfaction. To determine the association of trainees with clinic wait times and patient satisfaction scores in an academic center.Study DesignCross‐sectional study.MethodsWe recruited 266 study participants from an interdisciplinary Head and Neck Cancer outpatient clinic setting. Trained observers recorded observations related to wait times, time with individual health care practitioners, and total time spent in clinic. An 11‐question survey was given to patients at the end of their visit assessing each patient's satisfaction with their visit, subjective wait time, and their likelihood to recommend the health care provider.ResultsIncreased objective wait times were associated with new patients (p = 0.006) and based on the physician they saw (p < 0.001). Patients who saw a trainee spent less time waiting to see a physician (p = 0.023), more total time with a physician (p = 0.001), and reported higher wait time satisfaction scores (p = 0.001). There was no difference in total visit time if patients saw a trainee (p = 0.42). Patient satisfaction with wait time was correlated with all other aspects of patient satisfaction (p < 0.001). On multivariable analysis, the subjective wait time was associated with the likelihood to recommend (p < 0.001).ConclusionProlonged objective wait times in a multidisciplinary oncology outpatient setting were associated with several factors including specific physicians and new patient status. Trainee interaction with patients led to shorter wait times and improved patient satisfaction scores with wait times. Satisfaction with wait time was positively correlated with all aspects of patient satisfaction and ‘likelihood to recommend' scores.Level of EvidenceNA Laryngoscope, 134:178–184, 2024
Moss establishment in restoration: The role of moss production method and short‐term benefits of abscisic acid
Authors: Bowker Matthew A. , Doherty Kyle D. , Grover Henry S. , Antoninka Anita J. , Durham Rebecca A. , Ramsey Philip 
Published: June 2023

AbstractMosses may be useful in ecological restoration activities but are excluded from most native plant materials programs. Recent efforts have attempted to propagate mosses in controlled environments for deployment to boost the recovery of degraded field sites. Field re‐entry and establishment have proven challenging, possibly because the moss materials are not field‐ready. We compared the field establishment rates of mosses of the same species propagated using three methodologies carried out either in greenhouses or outdoors. In an attempt to chemically boost field‐readiness, we amended each with either sucrose, an osmoprotectant, or abscisic acid, a stress response phytohormone, or neither. Mosses grown outdoors with only one initial fall irrigation event lost at least 30% less cover than outdoor‐grown moss that was irrigated in spring and moss tissue grown in a fog chamber inside of a greenhouse. The addition of abscisic acid also induced a subtle difference, leading to about 10% less cover loss compared to controls. Ultimately, all treatments declined to only trace level moss cover at most after 3 years. From these results, we put forward the working hypothesis that growing methodologies more similar to field conditions and exposing mosses to environmental fluctuations are more likely to produce field‐ready moss materials. Abscisic acid addition is promising as a way to delay the mortality of mosses introduced into a desiccating environment. To translate short‐term relative differences to long‐term success, these practices may need to be combined with techniques that reduce the stress experienced in the field.
Prolific nitrite reoxidation across the Eastern Tropical North Pacific Ocean
Authors: Evans Natalya , Tichota Juliana , Moffett James W. , Devol Allan H. 
Published: June 2023

AbstractNitrite is a key intermediate during fixed nitrogen loss in the ocean, and it accumulates within marine Oxygen Deficient Zones (ODZ). ODZs are vast subsurface regions where nitrate is the dominant electron acceptor, and these regions host approximately 50% of the fixed nitrogen loss in the world's oceans. Nitrite accumulates in these waters, and recent research has discovered substantial reoxidation of nitrite back to nitrate, a significant process in the global nitrogen cycle. Partitioning between reduction and oxidation determines if marine fixed nitrogen is lost or recycled. Investigations into nitrite oxidation typically rely on results from incubations, which limits the spatiotemporal sampling coverage. Using basin‐scale data, we analyzed the ratios of nutrient regeneration within the three water masses that feed the Eastern Tropical North Pacific (ETNP) ODZ. Deviations in the ratios of nutrient regeneration from Redfield stoichiometry indicated prolific nitrite reoxidation across this region. We estimate that 79 ± 7% of the nitrite produced in the ODZ between the 26.2 and 26.4 kg m−3 isopycnals is reoxidized, whereas 54 ± 2% of the nitrite produced between the 26.7 and 26.9 kg m−3 isopycnals is reoxidized. Our analysis also illustrates discrete “metabolic switching points” from primarily aerobic to primary anaerobic processes, which occur in each water mass. We applied water mass analysis to repeat cruises on the WOCE P18 line from Baja California to 10°N, which revealed high spatiotemporal variability in nitrite reoxidation. These results confirm previous measurements of significant fixed nitrogen recycling across the ETNP; however, our analysis enables high‐resolution estimates of this process.
Photoluminescence Enhancement of InSe by Coupling with Circular Bragg Grating
Authors: Liu Yong , Zhong Qianqian , Liang Delang , Jiang Qi , Shuai Qin , Yang Xin , Yi Xiao , Sun Xingxia , Sun Ronghuan , Zhong Yangguang , Ge Cuihuan , Tan Qin , Luo Ziyu , Chen Shula , Pan Anlian 
Published: June 2023

AbstractIndium selenide (InSe) has attracted tremendous research interest due to its excellent optical and electronic properties. The direct bandgap of bulk InSe promises efficient carrier recombination in the near‐infrared (NIR) spectral range, holding great potential for NIR‐based optoelectronic device applications. However, the lowest energy transition in InSe involves out‐of‐plane optical dipoles, with resulting photoluminescence (PL) transmitted mainly along the layer plane. This limits both optical excitation and detection efficiency along the surface normal for practical device operation. To circumvent this issue, here, bulk InSe flake is coupled with circular Bragg grating (CBG) fabricated on silicon substrate by focused ion beam milling. A maximal 60‐fold PL enhancement is achieved, with Purcell effect‐facilitated carrier recombination and improved light out‐coupling via CBG contributing jointly. The same device architecture is further demonstrated to boost the second harmonic generation by a factor of 34. The results provide an effective way to enhance the optical performance of III–VI layered nanomaterials for both linear and nonlinear optoelectronic device applications.
Investigation of structural, electrical and tribological properties of duplex surface treated and aged Cu−Cr−Zr alloy
Authors: Celik I. , Yuksel O. 
Published: June 2023

AbstractIn this study, Cu−Cr−Zr alloy specimens were first subjected to aging heat treatment at 500 °C for two different durations (2 hours and 4 hours). Three different coating processes (silver, silver/copper duplex, and copper/silver duplex) were applied to the aged specimens using the physical vapor deposition method. Tensile test, microhardness measurement, wear test, electrical conductivity measurement and structural analysis (x‐ray diffraction and scanning electron microscope) were applied to the specimens. After the aging processes, the highest hardness and strength values of the Cu−Cr−Zr alloy were obtained in the specimens that were aged at 500 °C for 4 hours. When the wear resistance and electrical conductivity of the coated specimens were examined, it was determined that the specimens with duplex surface treatment performed better.
USP9X deubiquitinates and stabilizes CDC123 to promote breast carcinogenesis through regulating cell cycle
Authors: Song Nan , Deng Ling , Zeng Lijie , He Li , Liu Chunyan , Liu Ling , Fu Rong 
Published: June 2023

AbstractCell division cycle 123 (CDC123) has been implicated in a variety of human diseases. However, it remains unclear whether CDC123 plays a role in tumorigenesis and how its abundance is regulated. In this study, we found that CDC123 was highly expressed in breast cancer cells, and its high expression was positively correlated with a poor prognosis. Knowndown of CDC123 impaired the proliferation of breast cancer cells. Mechanistically, we identified a deubiquitinase, ubiquitin‐specific peptidase 9, X‐linked (USP9X), that could physically interact with and deubiquitinate K48‐linked ubiquitinated CDC123 at the K308 site. Therefore, the expression of CDC123 was positively correlated with USP9X in breast cancer cells. In addition, we found that deletion of either USP9X or CDC123 led to altered expression of cell cycle‐related genes and resulted in the accumulation of cells population in the G0/G1 phase, thereby suppressing cell proliferation. Treatment with the deubiquitinase inhibitor of USP9X, WP1130 (Degrasyn, a small molecule compound that USP9X deubiquitinase inhibitor), also led to the accumulation of breast cancer cells in the G0/G1 phase, but this effect could be rescued by overexpression of CDC123. Furthermore, our study revealed that the USP9X/CDC123 axis promotes the occurrence and development of breast cancer through regulating the cell cycle, and suggests that it may be a potential target for breast cancer intervention. In conclusion, our study demonstrates that USP9X is a key regulator of CDC123, providing a novel pathway for the maintenance of CDC123 abundance in cells, and supports USP9X/CDC123 as a potential target for breast cancer intervention through regulating the cell cycle.
Diagnosis of acute myocardial infarction using a combination of circulating circular RNA cZNF292 and clinical information based on machine learning
Authors: Zhou Qiulian , Boeckel Jes‐Niels , Yao Jianhua , Zhao Juan , Bai Yuzheng , Lv Yicheng , Hu Meiyu , Meng Danni , Xie Yuan , Yu Pujiao , Xi Peng , Xu Jiahong , Zhang Yi , Dimmeler Stefanie , Xiao Junjie 
Published: June 2023

AbstractCirculating circular RNAs (circRNAs) are emerging as novel biomarkers for cardiovascular diseases (CVDs). Machine learning can provide optimal predictions on the diagnosis of diseases. Here we performed a proof‐of‐concept study to determine if combining circRNAs with an artificial intelligence approach works in diagnosing CVD. We used acute myocardial infarction (AMI) as a model setup to prove the claim. We determined the expression level of five hypoxia‐induced circRNAs, including cZNF292, cAFF1, cDENND4C, cTHSD1, and cSRSF4, in the whole blood of coronary angiography positive AMI and negative non‐AMI patients. Based on feature selection by using lasso with 10‐fold cross validation, prediction model by logistic regression, and ROC curve analysis, we found that cZNF292 combined with clinical information (CM), including age, gender, body mass index, heart rate, and diastolic blood pressure, can predict AMI effectively. In a validation cohort, CM + cZNF292 can separate AMI and non‐AMI patients, unstable angina and AMI patients, acute coronary syndromes (ACS), and non‐ACS patients. RNA stability study demonstrated that cZNF292 was stable. Knockdown of cZNF292 in endothelial cells or cardiomyocytes showed anti‐apoptosis effects in oxygen glucose deprivation/reoxygenation. Thus, we identify circulating cZNF292 as a potential biomarker for AMI and construct a prediction model “CM + cZNF292.”