bluuebunny/crossref_metadata_2025_split huggingface dataset:
Membrane fusogenic nanoparticle‐based HLA‐peptide‐addressing universal T cell receptor‐engineered T (HAUL TCR‐T) cell therapy in solid tumor
Authors: Xu Ruihan , Wang Qin , Zhu Junmeng , Bei Yuncheng , Chu Yanhong , Sun Zhichen , Du Shiyao , Zhou Shujuan , Ding Naiqing , Meng Fanyan , Liu Baorui 
Published: August 2023

AbstractT cell receptor‐engineered T (TCR‐T) cell therapy has demonstrated therapeutic effects in basic research and clinical trials for treating solid tumors. Due to the peptide‐dependent recognition and the human leukocyte antigen (HLA)‐restriction, TCR‐T cell therapy is generally custom designed to target individual antigens. The lack of suitable universal targets for tumor cells significantly limits its clinical applications. Establishing a universal TCR‐T treatment strategy is of great significance. This study designed and evaluated the HLA‐peptide‐addressing universal (HAUL) TCR‐T cell therapy based on HLA‐peptide (pHLA) loaded membrance fusogenic deliver system. The pHLA‐NP‐based tumor cell membrane modification technology can transfer the pHLA onto the surface of tumor cells through membrane fusogenic nanoparticles. Then tumor cells are recognized and killed by TCR‐T cells specifically. The HAUL TCR‐T cell therapy technology is a universal technology that enables tumor cells to be identified and killed by specific TCR‐T cells, regardless of the HLA typing of tumor cells.
Guanylate‐binding proteins signature predicts favorable prognosis, immune‐hot microenvironment, and immunotherapy response in hepatocellular carcinoma
Authors: Ning Yumei , Fang Shilin , Fang Jun , Lin Kun , Nie Haihang , Xiong Peiling , Qiu Peishan , Zhao Qiu , Wang Haizhou , Wang Fan 
Published: August 2023

AbstractBackgroundThe role of guanylate‐binding proteins (GBPs) in various cancers has been elucidated recently. However, our knowledge of the clinical relevance and biological characteristics of GBPs in hepatocellular carcinoma (HCC) remains limited.MethodsA total of 955 HCC patients were enrolled from five independent public HCC cohorts. The role of GBP molecules in HCC was preliminarily investigated, and a GBP family signature, termed GBPs‐score, was constructed by principal component analysis to combine the GBP molecule values. We revealed the effects of GBP genes and GBPs‐score in HCC via well‐established bioinformatics methods and validated GBP1‐5 experimentally in a tissue microarray (TMA) cohort.ResultsGBPs molecules were closely associated with the prognosis of patients with HCC, and a high GBPs‐score highly inferred a favorable survival outcome. We also revealed high GBPs‐score was related to anti‐tumor immunity, the immune‐hot tumor microenvironment (TME), and immunotherapy response. Among the GBPs members, GBP1‐5 rather than GBP6/7 may be dominant in these fields. The TMA analysis based on immunohistochemistry showed positive correlations between GBP1‐5 and the immune‐hot TME with abundant infiltration of CD8+ T cells in HCC.ConclusionsOur integrative study revealed the genetic and immunologic characterizations of GBPs in HCC and highlighted their potential values as promising biomarkers for prognosis and immunotherapy.
Patient‐reported outcomes in older breast cancer survivors with and without prior chemotherapy treatment
Authors: Adesoye Taiwo , Liao Kai‐Ping , Peterson Susan , Li Liang , Zorzi Daria , Holmes Holly M. , Chavez‐MacGregor Mariana , Giordano Sharon H. 
Published: August 2023

AbstractBackgroundLittle is known about long‐term treatment‐related symptoms in older breast cancer survivors. We characterized long‐term patient‐reported symptoms and examined factors associated with the presence and severity of symptoms, and symptom interference with daily activities.MethodsTexas Cancer Registry (TCR) Medicare linkage data was used to identify breast cancer patients age 65 and older with local/regional stage disease diagnosed between 2012–2013. Symptom burden was assessed using breast‐specific items from the Patient‐Reported Outcomes version of the Common Terminology Criteria for Adverse Events (PRO‐CTCAE™). Demographic and clinical data also were collected. Logistic regression models were used to assess the association between symptom burden and respondent sociodemographic and clinical characteristics.ResultsOf 4448 eligible patients, 1594 (response‐rate 35.8%) completed questionnaires. Of these, 1245 eligible respondents were included in the analysis based on self‐reported data. Median time from diagnosis to survey completion was 68 months (IQR: 62–73). Most frequently reported symptoms were fatigue/lack of energy (76.8%), aching muscles (72.1%) and aching joints (72.5%). Receipt of chemotherapy was associated with higher symptom burden. Patients treated with adjuvant chemotherapy had higher risk of numbness/tingling (OR: 3.16; 95% CI: 2.36–4.24), hair loss (OR: 2.72; 95% CI: 2.05–3.60), and fatigue/lack of energy (OR: 1.80; 95% CI: 1.29–2.52). Similarly, patients who received chemotherapy were more likely to report the majority of symptoms as moderate to severe and as interfering with daily activities.ConclusionReceipt of chemotherapy is associated with significant symptom burden more than 5 years after breast cancer treatment. Long‐term chemotherapy impact should be discussed with patients in a shared‐decision making process and approaches to symptom management during survivorship care are needed.
Disparities in pediatric cancer survivorship care: A systematic review
Authors: Mobley Erin M. , Moke Diana J. , Milam Joel , Ochoa‐Dominguez Carol Y. , Stal Julia , Mitchell Halle , Aminzadeh Naghmeh , Bolshakova Maria , Mailhot Vega Raymond B. , Dinalo Jennifer , Motala Aneesa , Hempel Susanne 
Published: August 2023

AbstractBackgroundChildhood cancer survivors (CCS) experience many long‐term health problems that can be mitigated with recommended survivorship care. However, many CCS do not have access to survivorship care nor receive recommended survivorship care. We reviewed the empirical evidence of disparities in survivorship care for CCS.MethodsThis systematic review searched PubMed, CINAHL, and PsycINFO for studies on survivorship care for CCS (PROSPERO: CRD42021227965) and abstracted the reported presence or absence of disparities in care. We screened 7945 citations, and of those, we reviewed 2760 publications at full text.ResultsA total of 22 studies reported in 61 publications met inclusion criteria. Potential disparities by cancer treatment (N = 14), diagnosis (N = 13), sex (N = 13), and current age (N = 13) were frequently studied. There was high quality of evidence (QOE) of survivorship care disparities associated with non‐White race, Hispanic ethnicity, and being uninsured. Moderate QOE demonstrated disparities among CCS who were unemployed and older. Lower QOE was found for disparities based on cancer diagnosis, cancer treatment, age at diagnosis, time since diagnosis, sex, insurance type, income, educational attainment, and geographic area.ConclusionsWe found strong empirical evidence of disparities in survivorship care for CCS associated with race, ethnicity, and insurance status. Multiple other disparate groups, such as those by employment, income, insurance type, education, cancer diagnosis, age at diagnosis, time since diagnosis, cancer treatment, geographic area, sex, and self‐identified gender warrant further investigation. Prospective, multilevel research is needed to examine the role of other patient characteristics as potential disparities hindering adequate survivorship care in CCS.
Quinoline Hydrazone Derivatives as New Antibacterials against Multidrug Resistant Strains
Authors: Hodyna Diana , Kovalishyn Vasyl , Romanenko Yanina , Semenyuta Ivan , Blagodatny Volodymyr , Kachaeva Maryna , Brazhko Oleksandr , Metelytsia Larysa 
Published: August 2023

AbstractTo develop novel antimicrobial agents a series of 2(4)‐hydrazone derivatives of quinoline were designed, synthesized and tested. QSAR models of the antibacterial activity of quinoline derivatives were developed by the OCHEM web platform using different machine learning methods. A virtual set of quinoline derivatives was verified with a previously published classification model of anti‐E. coli activity and screened using the regression model of anti‐S. aureus activity. Selected and synthesized 2(4)‐hydrazone derivatives of quinoline exhibited antibacterial activity against the standard and antibiotic‐resistantS. aureusandE. colistrains in the range from 15 to 30 mm by the diameter of growth inhibition zones. Molecular docking showed the complex formation of the studied compounds into the catalytic domain of dihydrofolate reductase with an estimated binding affinity from −8.4 to −9.4 kcal/mol.
Aspirin‐induced urticaria in a recently diagnosed ischemic stroke patient: A case report and literature review
Authors: Dahal Abhinav , Gautam Sushant , Shakya Aliza , Pant Ashmita , Bhandari Kriti , Shrestha Shumneva , Shrestha Sajina , Shrestha Abhigan Babu 
Published: August 2023

Key Clinical MessageNSAIDs may be rare but an important cause of urticarial which should not be missed.AbstractThe aspirin and urticaria correlation has not been fully understood. The pharmacological inference is suspected to be the diversion of arachidonic acid metabolism. Aspirin sensitivity can aggravate preexisting chronic urticaria and in some instances causes acute urticaria. We report a case of a 53‐year‐old male, recently diagnosed with a stroke, who presented with complaints of multiple rashes over the trunk and upper extremities with aspirin. NSAIDs induced urticarial are usually neglected by physicians during diagnosis.
The Best of Both Worlds: Combining the Power of MICs and WCAs To Generate Stable and Crystalline CrI‐Tetracarbonyl Complexes with π‐Accepting Ligands
Authors: Bens Tobias , Walter Robert R. M. , Beerhues Julia , Schmitt Manuel , Krossing Ingo , Sarkar Biprajit 
Published: August 2023

AbstractInvited for the cover of this issue are Biprajit Sarkar and co‐workers at the University of Stuttgart and University of Freiburg. In the image, the solar flare represents the non‐innocence (fluorine‐specific interactions) of the counterion, and the black hole at the metal center illustrates the oxidation/electron deficiency of the Cr‐center, while the electron “gets lost” in the space (oxidation agent). Read the full text of the article at 10.1002/chem.202301205.
Effect of infant viral respiratory disease on childhood asthma in a non‐industrialized setting
Authors: Atwell Jessica , Chico Martha , Vaca Maritza , Arévalo‐Cortes Andrea , Karron Ruth , Cooper Philip J. 
Published: August 2023

AbstractBackgroundThere are limited data from non‐industrialized settings on the effects of early life viral respiratory disease on childhood respiratory illness. We followed a birth cohort in tropical Ecuador to understand how early viral respiratory disease, in the context of exposures affecting airway inflammation including ascariasis, affect wheezing illness, asthma, and rhinoconjunctivitis in later childhood.MethodsA surveillance cohort nested within a birth cohort was monitored for respiratory infections during the first 2 years in rural Ecuador and followed for 8 years for the development of wheeze and rhinoconjunctivitis. Nasal swabs were examined for viruses by polymerase chain reaction and respiratory symptom data on recent wheeze and rhinoconjunctivitis were collected by periodic questionnaires at 3, 5, and 8 years. Stools from pregnant mothers and periodically from children aged 2 years were examined microscopically for soil‐transmitted helminths. Atopy was measured by allergen skin prick testing at 2 years. Spirometry, fractional exhaled nitric oxide measurement, and nasal washes were performed at 8 years. Associations between clinically significant respiratory disease (CSRD) and wheezing or rhinoconjunctivitis at 3, 5, and 8 years were estimated using multivariable logistic regression.ResultsFour hundred and twenty six children were followed of which 67.7% had at least one CSRD episode; 12% had respiratory syncytial virus (RSV)+CSRD and 36% had rhinovirus (RHV)+CSRD. All‐cause CSRD was associated with increased wheeze at 3 (OR 2.33 [95% confidence intervals (CI) 1.23–4.40]) and 5 (OR: 2.12 [95% CI 1.12–4.01]) years. RHV+CSRD was more strongly associated with wheeze at 3 years in STH‐infected (STH‐infected [OR 13.41, 95% CI 1.56–115.64] vs. uninfected [OR 1.68, 95% CI 0.73–3.84]) and SPT+ (SPT+ [OR 9.42, 95% CI 1.88–47.15] versus SPT‐ [OR 1.92, 95% CI 0.84–4.38]) children. No associations were observed between CSRD and rhinoconjunctivitis.DiscussionCSRD was significantly associated with childhood wheeze with stronger associations observed for RHV+CSRD in SPT+ and STH‐infected children.
Persistence of patient‐detected breast cancer over time: 1990–2019
Authors: Malmgren Judith A. , Atwood Mary K. , Kaplan Henry G. 
Published: August 2023

AbstractBackgroundThe continued presentation of patient‐detected breast cancer (BC) and associated characteristics over time is understudied.MethodsIn a large institutional cohort of first primary stage 0–IV patients with BC in 1990–2019 (n = 15,827), diagnostic method (patient‐detected [PtDBC] [n = 5844]; mammography‐detected [MamDBC] [nondiagnostic] [n = 9248]; and physician‐detected [PhysDBC] [n = 736]) and patient and tumor characteristics including age, race, TNM stage, and hormone‐receptor status were reviewed. Pearson χ2 tests for bivariate comparisons and logistic regression for patient detection–associated factors were used.ResultsIn a cohort from 1990 to 2019, the proportion aged 50–74 years (55%–63%; p < .001) and non‐White race (9%–37%; p < .001) increased over time. Percentage PtDBC decreased over time but case numbers increased (1990–1999: 44% [n = 1399]; 2010–2019: 34% [n = 2349]; p < .001). Excluding stage 0, PtDBC declined from 47% to 41% over time (p < .001). In 2010–2019, 21% of cases were stage 0, 91% of which were mammography detected (n = 1439). Seventy percent of patient‐detected cases were stage II–IV (stage II, 44%; stage III, 20%; stage IV, 6%; p < .001). In adjusted logistic regression, the odds of PtDBC decreased over time (2000–2009: odds ratio [OR], .65 [95% CI, .58–.72]; 2010–2019: OR, .54 [95% CI, .49–.60]), with age <40 years OR, 15.81, and Black and non‐White other at 50% increased risk.ConclusionsThe relative proportion of PtDBC decreased to a constant 34%–40% of total cases after 1990–1999. PtDBC case numbers increased in subsequent years (2000–2019), and were consistently higher stage. Interval cancers, mammography‐screening uptake, breast health awareness of age groups outside screening guidelines, and underserved socioeconomic groups may be related to the continued significant PtDBC incidence.Plain Language Summary

After decades of mammography‐screening availability, symptomatic patient‐detected breast cancer declined over time from 44% to a persistent rate of 34% in our institutional cohort.
The persistence of patient‐detected breast cancer over time presents a difficult situation for patients and care givers without clear diagnosis pathways for younger and older women outside recommended screening guidelines, who often present with higher stage and more lethal characteristics.
More timely diagnosis and treatment including breast health awareness, prompt presentation of breast problems, outreach to younger age and minority groups, and provision of specialized training and care delivery for symptomatic patient‐detected breast cancer are needed.
Enzymatic and Non‐Enzymatic Uric Acid Electrochemical Biosensors: A Review
Authors: Sun Miao , Cui Chuanjin , Chen Hongshuo , Wang Dengling , Zhang Wensi , Guo Wenjin 
Published: August 2023

AbstractIn recent years, the development of electrochemical biosensors for uric acid has made great achievements. Firstly, uric acid electrochemical biosensors were classified according to their reaction mechanism. Then, the reaction mechanism of the uric acid sensor and the application of nano‐modified materials were deeply analyzed from the perspective of non‐enzyme and enzymes. In this paper, the catalytic oxidation capacity, enzyme adsorption effect, conductivity, robustness, detection range, and detection limit of uric acid sensors were discussed and compared. Finally, the advantages of acid‐sensitive electrochemical biosensors were summarized, and the constructive recommendations were proposed for improving the deficiencies of acid biosensors. The potential for further development in this area was also discussed.
Kinase‐catalyzed Biotinylation with Inactivated Lysates for Discovery of Substrates (K‐BILDS)
Authors: Gary Chelsea R. , Pflum Mary Kay H. 
Published: August 2023

AbstractProtein phosphorylation is catalyzed by kinases to regulate a large variety of cellular activities, including growth and signal transduction. Methods to identify kinase substrates are crucial to fully understand phosphorylation‐mediated cellular events and disease states. Here, we report a set of protocols to identify substrates of a target kinase using Kinase‐catalyzed Biotinylation with Inactivated Lysates for Discovery of Substrates (K‐BILDS). As described in these protocols, K‐BILDS involves inactivation of endogenous kinases in lysates, followed by addition of an active exogenous kinase and the γ‐phosphate‐modified ATP analog ATP‐biotin for kinase‐catalyzed biotinylation of cellular substrates. Avidin enrichment isolates biotinylated substrates of the active kinase, which can be monitored by western blot. Substrates of the target kinase can also be discovered using mass spectrometry analysis. Key advantages of K‐BILDS include compatibility with any lysate, tissue homogenate, or complex mixture of biological relevance and any active kinase of interest. K‐BILDS is a versatile method for studying or discovering substrates of a kinase of interest to characterize biological pathways thoroughly. © 2023 Wiley Periodicals LLC.Basic Protocol 1: FSBA treatment of lysates to inactivate kinasesBasic Protocol 2: Kinase‐catalyzed Biotinylation with Inactivated Lysates for Discovery of Substrates (K‐BILDS)
Humanized Mouse Models for Immuno‐oncology Drug Discovery
Authors: Kumari Rajendra , Feuer Gerold , Bourré Ludovic 
Published: August 2023

AbstractBreakthroughs in cancer treatment with immunotherapeutics have provided long‐term patient benefits for many different types of cancer. However, complete response is not achieved in many patients and tumor types, and the mechanisms underlying this lack of response are poorly understood. Despite this, numerous new targets, therapeutics, and drug combinations are being developed and tested in clinical trials. Preclinical models that recapitulate the complex human tumor microenvironment and the interplay between tumor and immune cells within the cancer‐immunity cycle are needed to improve our understanding and screen new therapeutics for efficacy and safety/toxicity. Humanized mice, encompassing human tumors and human immune cells engrafted on immunodeficient mice, have been widely used for many years in immuno‐oncology, with developments to improve both the humanization and the translational value central to the next generation of models. In this overview, we discuss recent advances in humanized models relevant to immuno‐oncology drug discovery, the advantages and limitations of such models, the application of humanized models for efficacy and safety assessments of immunotherapeutics, and the potential opportunities. © 2023 Crown Bioscience. Current Protocols published by Wiley Periodicals LLC.
Synthesis of Double Defects in g‐C3N4 to Enhance the H2O2 Production by Dual‐Electron O2 Reduction
Authors: Li Renjie , Deng Qunfen , Chen Anli , Zhong Yujia , Yang Rui 
Published: August 2023

AbstractIn this work, the graphitic carbon nitride with −C≡N defects and S‐defects (N2−SCN‐4) was constructed. The H2O2 production efficiency of N2−SCN‐4 was 1423.3 μmol g−1 h−1 under the visible light (λ≥420 nm) irradiation, which was 15.4 times that of pristine g‐C3N4. The −C≡N groups promote the adsorption of H+ and the S‐defects provide the active center for the adsorption and activation of O2. Furthermore, the surface morphology, microstructure, and photoelectric chemical properties of samples were investigated by a series of characterizations, and the response range of N2−SCN‐4 to visible light increases obviously. Meanwhile, the efficiency of photo‐produced charge separation and the selectivity of H2O2 production were discussed in detail. The experimental and characterization results confirmed that the charge separation efficiency and the selectivity of the 2e− O2 reduction reaction (ORR) were improved under the synergistic effect of the double defects. This work provides a strategy for improving the photocatalytic performance of photocatalysts.
Amination of Isonitriles in Water: Urea Synthesis under Biocompatible Conditions
Authors: Zhang Qi , Wang Xueting , Chen Shi‐Wu , Tang Meng , Zhang Qian , Xia Yiwen 
Published: August 2023

AbstractBioorthogonal reactions have been widely used to track biomolecules in living systems. Due to stringent requirements of physiological conditions, enriching the toolkit of bioorthogonal reactions remains the most important and challenging issue. Herein, the biocompatible ligation of isonitriles and amines to ureas in neutral aqueous medium was developed for the first time. The ligation showed benign nature of biocompatibility, broad substrate scope, and specific chemoselectivity. Meanwhile, urea as a natural linkage, its derivatives played an important role in medicinal chemistry. The second‐order reaction rate constant (k2) was determined, which was higher or comparable to that of Staudinger ligation and strain‐promoted azide‐alkyne cycloaddition (SPAAC). Direct labeling of Ac4GlcN has been achieved in Hep G2 cells.
Numerical simulation of fracture propagation in deep coal seam reservoirs
Authors: Tian Zhongzheng , Xiong Zhuang , Wei Yuhua , Ma Shou , Hu Xiaodong 
Published: August 2023

AbstractAs an unconventional oil and gas resource, coalbed methane has become a key area of unconventional oil and gas exploration worldwide, and hydraulic fracturing is the critical technology for the efficient stimulation of coalbed methane. However, due to the strong heterogeneity of deep coal seam reservoirs and the development of natural cleat fractures, the fracture propagation path is quite complex and difficult to capture. In this study, we propose a hydraulic fracture propagation simulation method using the ABAQUS Software for natural cleat fracture networks and a fluid–solid fully coupled two‐dimensional finite element model with the global embedded cohesive zone model (CZM). CZM is established to simulate the propagation of hydraulic fractures in the heterogeneous combination of coal rock and mudstone. The accuracy of this model is verified by using Blanton's experiment criterion. Finally, the model is used to simulate the influence of cleat angle, cleat density, length ratio of coal rock and mudstone combination, and construction displacement on the propagation behavior of hydraulic fractures. This study aims to deepen the understanding of the mechanism of hydraulic fracture propagation in deep coal seams and provides guidance for deep coal seam fracturing and hydraulic fracturing design.