bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop‐P‐methyl resistance in Digitaria sanguinalis
Authors: Liu Xiangying , Hou Zhenlin , Zhang Yuying , Merchant Austin , Zhong Mei‐e , Ma Guolan , Zeng Qing , Wu Lamei , Zhou Xuguo , Luo Kun , Ding Chunxia 
Published: May 2023

AbstractBackgroundHaloxyfop‐P‐methyl, an acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicide, has been extensively used to control grass weeds. Widespread use of haloxyfop‐P‐methyl in cotton fields in China has led to the development of glutathione transferase (GST)‐mediated resistance in Digitaria sanguinalis. An RNA‐seq analysis identified DsGSTU1, a tau class glutathione transferase from the D. sanguinalis transcriptome as a potential candidate. Here, we cloned DsGSTU1 from D. sanguinalis young leaf tissues and subsequently characterized DsGSTU1 by a combination of sequence analysis, as well as functional heterologous expression in rice.RESULTSThe full‐length coding DNA sequence (CDS) of DsGSTU1 is 717 bp in length. Higher DsGSTU1 expression was observed in haloxyfop‐P‐methyl‐resistant (HR) D. sanguinalis than in haloxyfop‐P‐methyl‐susceptible (HS) plants. Overexpression of the DsGSTU1 gene was confirmed by transformation into the wild‐type (WT) Nipponbare rice with pBWA(V)HS, a recombinant expression vector. GST activity in transgenic rice seedlings was 1.18–1.40‐fold higher than the WT rice seedlings before and after haloxyfop‐P‐methyl treatment, respectively. Additionally, transgenic rice seedlings overexpressing DsGSTU1 were less sensitive to haloxyfop‐P‐methyl.CONCLUSIONOur combined findings suggest that DsGSTU1 is involved in metabolic resistance to haloxyfop‐P‐methyl in D. sanguinalis. A better understanding of the major genes contributing to herbicide‐resistant D. sanguinalis facilitates the development of resistance management strategies for this global invasive grass weed. © 2023 Society of Chemical Industry.
Joint optimization of bi‐level imperfect maintenance and spare parts inventory considering order quantity
Authors: He Yong , Wang Hong , Zhao Xiujie 
Published: May 2023

AbstractThe joint optimization of various maintenance actions and the supply of spare parts has received extensive attention in recent years owing to its advantage in reducing the overall operational costs of industrial systems. In this study, a joint optimization model is proposed considering bi‐level imperfect preventive maintenance and spare part ordering. A virtual age reduction model that considers bi‐level imperfect preventive maintenance (PM) is established. Upon each system shutdown, the PM level is determined based on the cost‐effectiveness ratio. Furthermore, an improved (s, S) inventory policy is proposed utilizing the information of the remaining number of PM and accumulated consumption of spare parts. Finally, the joint optimization models consisting of different PM policies and spare parts inventory policies are solved. Case studies show that compared with the traditional (s, S) inventory policy, the proposed joint optimization model can coordinate the ordering quantity of spare parts with the PM policy, and reduce the expected maintenance cost. A sensitivity analysis of the cost parameters is performed to explore their impact on the maintenance economy.
Toward sustainable development: Does digitalization narrow the gender gap in the labor market?
Authors: Yin Zi Hui , Zhang Ting Hui , Choi Chang Hwan 
Published: May 2023

AbstractThe direct and moderating impact of digitalization on the gender gap in the labor force participation rate is explored in this study. By analyzing the panel data of the G20 countries for the period of 2006–2021, we find that (1) the rising level of digitalization tends to narrow the gender gap in the labor force participation rate, with a greater gender gap‐narrowing effect in high‐income countries than in upper‐middle and lower‐middle income countries. (2) The interaction between digitalization and globalization has a small impact on the gender gap in the labor force participation rate in the full sample of the G20 countries, but the results are significantly different in countries divided by income level: the interaction is more likely to narrow the gender gap in the high‐income countries but widen the gender gap in upper‐middle and lower‐middle income countries. The gender gap‐widening effect is greater in upper‐middle income than in lower‐middle income countries. (3) Digitalization is more likely to increase the female employment rate and reduce the female unemployment rate, while it has the opposite effect on males; the female employment improvement effect is observed in the services sector.
Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh
Authors: Saha Otun , Basri Rabeya , Hossain Md. Anwar , Sultana Munawar 
Published: May 2023

AbstractBackgroundResistance to multiple drugs in Klebsiella pneumoniae (KPN) is an important issue in human and animal medicine. KPN phenotypic and genotypic aspects in poultry samples have not been comprehensively explored in Bangladesh.MethodsThis research focused on the prevalence of antibiotic resistance and the characterization of KPN in Bangladeshi poultry isolates using both phenotypic and genotypic approaches.ResultsA total of 32 poultry samples were randomly obtained from a commercial poultry farm in Narsingdi, Bangladesh, and 43.90% (18/41) of the isolates were confirmed to be KPN, whereas all isolates were biofilm producers. The antibiotic sensitivity test revealed the most remarkable (100%) antibiotic resistance level against Ampicillin, Doxycycline and Tetracycline while remaining susceptible to Doripenem, Meropenem, Cefoxitin and Polymyxin B. Resistance to Nalidixic acid, Nitrofurantoin, Trimethoprim, Levofloxacin, Ciprofloxacin, Cefuroxime and Chloramphenicol ranges from 18% to 70%. Minimum inhibitory concentrations for carbapenem‐resistant KPN ranged from 128 to 512 mg/mL for Meropenem, Imipenem, Gentamycin and Ciprofloxacin, respectively. [Correction added on 15 June 2023, after first online publication: 512 g/mL was corrected to 512 mg/mL in the preceding sentence]. Carbapenemase‐producing KPN isolates harboured a single or multiple β‐lactamase genes, blaSIM‐1, blaIMP‐4 and blaOXA‐48 as well as one ESBL gene (blaTEM) and plasmid‐mediated quinolone resistance gene (qnrB). Furthermore, Cr and Co outperformed Cu and Zn in antibacterial effectiveness.ConclusionsThe results of this investigation showed that the high prevalence of multidrug‐resistant pathogenic KPN in our chosen geographic location had displayed sensitivity to FOX/PB/Cr/Co, which might be regarded as an alternate treatment to reduce pressure of use on carbapenems.
Conclusion: Why Did the UK, US and Australia Have Different E-cigarette Policies?
Authors: Berridge Virginia , Fairchild Amy L. , Morphett Kylie , Gartner Coral , Hall Wayne , Bayer Ronald 
Published: May 2023

AbstractWe sought to understand why three countries with similar political systems and similar anti-smoking policy histories developed such very different policies towards e-cigarettes. All appealed to a value-free concept of “evidence” in making use of precautionary and harm reduction principles to deal with the remaining uncertainties in the evidence. Yet policy processes were mediated by important contextual factors. These included: the nature and role of the state in each country; the political parties in power at the time e-cigarettes were first introduced; the role played by existing regulatory institutions in dealing with e-cigarettes; longer-term changes in ways of thinking about tobacco smoking within public health; the specific pre-history of tobacco control policy, nicotine and smoking cessation services; the organisation of professional and activist networks that were in favour of and against e-cigarettes; the uses of fear to discourage e-cigarette use; and the influence (or lack thereof) of harm reduction ideas from drug or AIDS policy on tobacco control policy. The object of policy also differed between countries from protecting the smoker to protecting children and young people.
Friendly Friction
Authors: Kvalnes Øyvind 
Published: May 2023

AbstractThis chapter introduces friendly friction as the first of five qualities that can be found in well-functioning communication climates. To establish friendly friction in an organisation, it is necessary to avoid the poles of having unfriendly friction and friendliness without friction. Social contagion poses an obstacle to creating a productive communication climate in that unfriendly friction encountered on social media or within an organisation can spread. Experiences of friendliness without friction can also create more of the same. However, social contagion can also help to establish and maintain friendly friction as a quality in the workplace through exposure to practices where people provide that kind of input to each other.
Zusammenfassung und Abstract
Authors: Becker-Hingst Nadine 
Published: May 2023

ZusammenfassungDepressionen gehören zu den häufigsten psychischen Erkrankungen im höheren Erwachsenenalter. Bei manchen Patient:innen entwickelt sich die Depression bereits in jüngeren Jahren und setzt sich im Alter fort. Bei vielen anderen Betroffenen tritt die depressive Störung allerdings erstmals im höheren Alter auf. In beiden Fällen geht die Erkrankung mit einem massiven Verlust an Lebensqualität, starken funktionellen Beeinträchtigungen und einem hohen Suizidrisiko einher.
Diskussion und Ausblick
Authors: Becker-Hingst Nadine 
Published: May 2023

ZusammenfassungIm Folgenden werden die Ergebnisse zum Zusammenhang zwischen Emotionsregulation und Depression im Alter diskutiert und zusammengefasst. Es werden Stärken und Limitationen dieser Untersuchung aufgeführt und aus den Limitationen zentrale Implikationen für zukünftige Forschungsvorhaben abgeleitet. Abschließend werden theoretische und klinisch-praktische Schlussfolgerungen aus den Ergebnissen gezogen.
Tumor cell-intrinsic and tumor microenvironmental conditions co-determine signaling by the glycoimmune checkpoint receptor Siglec-7
Authors: van Houtum Eline J. H. , Kers-Rebel Esther D. , Looman Maaike W. , Hooijberg Erik , Büll Christian , Granado Daniel , Cornelissen Lenneke A. M. , Adema Gosse J. 
Published: May 2023

AbstractTumors create an immunosuppressive tumor microenvironment by altering protein expression, but also by changing their glycosylation status, like altered expression of sialoglycans. Sialoglycans are capped with sialic acid sugar residues and are recognized by Siglec immune receptors. Siglec-7 is an inhibitory immune receptor similar to PD-1, and is emerging as glycoimmune checkpoint exploited by cancer cells to evade the immune system. However, the exact cellular and molecular conditions required for Siglec-7-mediated immune cell inhibition remain largely unknown. Here, we report on the development of a chimeric Siglec-7 cell system that enables dissection of Siglec-7 signaling, rather than Siglec-7 binding. Antibody-induced clustering, sialic acid-containing polymers, and highly sialylated erythrocytes effectively induced Siglec-7 signaling, thereby validating functionality of this reporter system. Moreover, the system reveals tumor cell-dependent Siglec-7 signaling. Tumor-associated conditions important for Siglec-7 signaling were defined, such as Siglec-7 ligand expression levels, presence of the known Siglec-7 ligand CD43, and sialic acid availability for sialylation of glycans. Importantly, therapeutic targeting of the Siglec-7/sialic acid axis using a sialyltransferase inhibitor resulted in strong reduction of Siglec-7 signaling. In conclusion, using a newly established cellular tool, we defined a set of tumor-associated conditions that influence Siglec-7 signaling. Moreover, the system allows to assess the efficacy of novel cancer drugs interfering with the Siglec-7/sialic acid axis as immunotherapy to treat cancer.
Epidemiologie seelischen Wohlbefindens von Kindern und Jugendlichen in Deutschland. Ergebnisse aus 3 Studien vor und während der COVID-19-Pandemie
Authors: Reiß Franziska , Kaman Anne , Napp Ann-Kathrin , Devine Janine , Li Lydia Y. , Strelow Lisa , Erhart Michael , Hölling Heike , Schlack Robert , Ravens-Sieberer Ulrike 
Published: May 2023

Zusammenfassung
Hintergrund
Ein kontinuierliches bundesweites Gesundheitsmonitoring ist wichtig, um das Wohlbefinden von Kindern und Jugendlichen im Blick zu behalten und Entwicklungsverläufe abzubilden. Anhand der Ergebnisse von 3 ausgewählten epidemiologischen Studien werden Entwicklungen zum kindlichen Wohlbefinden der letzten 20 Jahre vorgestellt.

Methodik
Datengrundlage bilden (1.) die bevölkerungsbezogene BEfragung zum seeLischen WohLbefinden und VerhAlten (BELLA-Studie, 2003–2017, N = 1500–3000), die ein Modul der KiGGS-Studie ist, (2.) die COrona und PSYche Studie (COPSY, 2020–2022, N = 1600–1700), die auf der BELLA-Studie aufbaut, und (3.) die internationale Health-Behaviour in School-aged Children Studie (HBSC, 2002–2018, N = 4300–7300). Das Wohlbefinden wurde bei 7‑ bis 17-Jährigen mittels der Indikatoren gesundheitsbezogene Lebensqualität (KIDSCREEN-10), Lebenszufriedenheit (Cantril Ladder) und psychische Auffälligkeiten (Strenghts and Difficulties Questionnaire (SDQ), Screen for Child Anxiety Related Emotional Disorders (SCARED) und Center for Epidemiological Studies Depression Scale for Children (CES-DC)) erfasst.

Ergebnisse
Insgesamt zeigen Kinder und Jugendliche präpandemisch (2002–2018) eine konstant hohe gesundheitsbezogene Lebensqualität und eine hohe allgemeine Lebenszufriedenheit, die sich mit Beginn der COVID-19-Pandemie 2020 zunächst verschlechterte. 2 Jahre später zeigen sich Verbesserungen, die jedoch noch nicht das Ausgangsniveau erreichen. Psychische Auffälligkeiten, ängstliche und depressive Symptome nahmen mit Pandemiebeginn um bis zu 12 Prozentpunkte zu und zeigen auch 2 Jahre nach Pandemiebeginn noch höhere Werte als präpandemische Studien.

Diskussion
Die Epidemiologie kindlichen Wohlbefindens bietet eine notwendige Datengrundlage, um den Unterstützungsbedarf von Kindern und Jugendlichen zu erfassen und auf dieser Basis Maßnahmen der Gesundheitsförderung, Prävention und Intervention zu entwickeln.
In vivo evaluation of a novel 18F-labeled PET radioligand for translocator protein 18 kDa (TSPO) in monkey brain
Authors: Yan Xuefeng , Siméon Fabrice G. , Liow Jeih-San , Morse Cheryl L. , Montero Santamaria Jose A. , Jenkins Madeline , Manly Lester S. , Van Buskirk Maia , Zoghbi Sami S. , Pike Victor W. , Innis Robert B. , Zanotti-Fregonara Paolo 
Published: May 2023

Abstract
Purpose
[18F]SF51 was previously found to have high binding affinity and selectivity for 18 kDa translocator protein (TSPO) in mouse brain. This study sought to assess the ability of [18F]SF51 to quantify TSPO in rhesus monkey brain.

Methods
Positron emission tomography (PET) imaging was performed in monkey brain (n = 3) at baseline and after pre-blockade with the TSPO ligands PK11195 and PBR28. TSPO binding was calculated as total distribution volume corrected for free parent fraction in plasma (VT/fP) using a two-tissue compartment model. Receptor occupancy and nondisplaceable uptake were determined via Lassen plot. Binding potential (BPND) was calculated as the ratio of specific binding to nondisplaceable uptake. Time stability of VT was used as an indirect probe to detect radiometabolite accumulation in the brain. In vivo and ex vivo experiments were performed in mice to determine the distribution of the radioligand.

Results
After [18F]SF51 injection, the concentration of brain radioactivity peaked at 2.0 standardized uptake value (SUV) at ~ 10 min and declined to 30% of the peak at 180 min. VT/fP at baseline was generally high (203 ± 15 mL· cm−3) and decreased by ~ 90% after blockade with PK11195. BPND of the whole brain was 7.6 ± 4.3. VT values reached levels similar to terminal 180-min values by 100 min and remained relatively stable thereafter with excellent identifiability (standard errors < 5%), suggesting that no significant radiometabolites accumulated in the brain. Ex vivo experiments in mouse brain showed that 96% of radioactivity was parent. No significant uptake was observed in the skull, suggesting a lack of defluorination in vivo.

Conclusion
The results demonstrate that [18F]SF51 is an excellent radioligand that can quantify TSPO with a good ratio of specific to nondisplaceable uptake and has minimal radiometabolite accumulation in brain. Collectively, the results suggest that [18F]SF51 warrants further evaluation in humans.
Rodrigues’ Descendants of a Polynomial and Boutroux Curves
Authors: Bøgvad Rikard , Hägg Christian , Shapiro Boris 
Published: May 2023

AbstractMotivated by the classical Rodrigues’ formula, we study below the root asymptotic of the polynomial sequence $$\begin{aligned} {\mathcal {R}}_{[\alpha n],n,P}(z)=\frac{\mathop {}\!\textrm{d}^{[\alpha n]}P^n(z)}{\mathop {}\!\textrm{d}z^{[\alpha n]}}, n= 0,1,\dots \end{aligned}$$

R

[
α
n
]
,
n
,
P

(
z
)

=

d

[
α
n
]

P
n

(
z
)

d

z

[
α
n
]

,
n
=
0
,
1
,
⋯

where P(z) is a fixed univariate polynomial, $$\alpha $$
α
is a fixed positive number smaller than $$\deg P$$

deg
P

, and $$[\alpha n]$$

[
α
n
]

stands for the integer part of $$\alpha n$$

α
n

. Our description of this asymptotic is expressed in terms of an explicit harmonic function uniquely determined by the plane rational curve emerging from the application of the saddle point method to the integral representation of the latter polynomials using Cauchy’s formula for higher derivatives. As a consequence of our method, we conclude that this curve is birationally equivalent to the zero locus of the bivariate algebraic equation satisfied by the Cauchy transform of the asymptotic root-counting measure for the latter polynomial sequence. We show that this harmonic function is also associated with an abelian differential having only purely imaginary periods and the latter plane curve belongs to the class of Boutroux curves initially introduced in Bertola (Anal Math Phys 1: 167–211, 2011), Bertola and Mo (Adv Math 220(1): 154–218, 2009). As an additional relevant piece of information, we derive a linear ordinary differential equation satisfied by $$\{{\mathcal {R}}_{[\alpha n],n,P}(z)\}$$

{

R

[
α
n
]
,
n
,
P

(
z
)

}

as well as higher derivatives of powers of more general functions.
Real-time MRI: a new tool of radiologic imaging in small children
Authors: Hirsch Franz Wolfgang , Frahm Jens , Sorge Ina , Klee Dirk , Prenzel Freerk , Krause Matthias , Lacher Martin , Voit Dirk , Gräfe Daniel 
Published: May 2023

AbstractReal-time MRI (rt-MRI) in children is a new imaging technique that combines the advantages of US — at frame rates of up to 50 images per second — with the quality and features of MRI. Although still subject of research, it has become a standard tool in the diagnostic portfolio of two pediatric radiology departments in Germany. Based on ultrashort acquisition times, any detrimental effects of macroscopic movements of the child and the physiological movements of the organs are negligible. Especially in pediatric brain imaging, rt-MRI has already proven its value. With suitable indications, rt-MRI can reduce anesthesia and sedation examinations in children below 6 years of age by 40% due to its very short examination time and its robustness to motion. There is a high level of acceptance among parents and referrers when diagnostic possibilities and limitations are communicated correctly. Conclusion: Completely new diagnostic possibilities arise in the imaging of the moving lung, the beating heart, joint movements, and speaking and swallowing, as demonstrated in this video-backed review.

What is known:• MRI in moving children has been burdened with severe artifacts.• Gross motion usually has to be handled by sedation and periodic motion of the heart and lungs has to be compensated with time-consuming techniques until now.

What is new:• Real-time MRI allows image acquisition with up to 50 frames per second similar to ultrasound frame rate.• Real-time MRI proofs to be very promising for imaging children, reducing examination time and sedation rate drastically.
miRNAs contributing to the repair of tendon injury
Authors: Lyu Kexin , Liu Xinyue , Liu Tianzhu , Lu Jingwei , Jiang Li , Chen Yixuan , Long Longhai , Wang Xiaoqiang , Shi Houyin , Wang Fan , Li Sen 
Published: May 2023

AbstractTendon injury is one of the most common disorders of the musculoskeletal system, with a higher likelihood of occurrence in elderly individuals and athletes. In posthealing tendons, two undesirable consequences, tissue fibrosis and a reduction in mechanical properties, usually occur, resulting in an increased probability of rerupture or reinjury; thus, it is necessary to propose an appropriate treatment. Currently, most methods do not sufficiently modulate the tendon healing process and restore the function and structure of the injured tendon to those of a normal tendon, since there is still inadequate information about the effects of multiple cellular and other relevant signaling pathways on tendon healing and how the expression of their components is regulated. microRNAs are vital targets for promoting tendon repair and can modulate the expression of biological components in signaling pathways involved in various physiological and pathological responses. miRNAs are a type of noncoding ribonucleic acid essential for regulating processes such as cell proliferation, differentiation, migration and apoptosis; inflammatory responses; vascularization; fibrosis; and tissue repair. This article focuses on the biogenesis response of miRNAs while presenting their mechanisms in tendon healing with perspectives and suggestions.
Functional Prototype for the Validation of the Innovative Metal Additive Manufacturing SLEDM Process
Authors: Haas Franz , Tiefnig Raphael 
Published: May 2023

AbstractAdditive manufacturing has found its way into industrial series production. However, market growth has only been possible through the introduction of new AM-technologies and the continuous improvement of existing processes. This contribution covers the practical implementation of a prototype for research and validation of the unique SLEDM process patented by TU Graz. The demonstrator includes a feature for powder coating for the melting process as well as the concept of power input via an LED-based light source. The prototype enables a now simple and fast melting of different low-melting powder materials, such as tin, zinc, and aluminum, within an inert gas atmosphere. In addition, the experimental setup forms the basis for the development and adaptation of a suitable light source for the next generation SLEDM process. The goal is to test the additive manufacturing concept with the innovative LED light source and analyze the produced metal parts. The evaluation of the recorded measurement data and the analysis of the test results provide important knowledge about the potential of the SLEDM process for further research and industrial manufacturing. In the future, the individual control of the LED-cells in the matrix can be utilized for optimized temperature profiles in the melting zone and for the reduction of the energy demand of the machine. Overall SLEDM helps to fulfil the targets of sustainable production addressing both the material and energy aspect.