bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lung cancer and miRNAs: a possible remedy for anti-metastatic, therapeutic and diagnostic applications
Authors: Dai Chun , Chen Huangkun , Wang Feng , Hua Zhaolai , Xia Lin , Yuan Lijie , Qin Lei 
Published: February 2023

Abstract
Background Lung cancer is often diagnosed as stage IV metastatic disease and is the leading cause of cancer-related death worldwide. Expression of micro-ribonucleic acids (microRNAs, miRNAs) and long-noncoding RNAs (lncRNAs) has been associated with metastasis in various tumor types. The present study investigates the role of miRNA and lncRNA in metastatic lung cancer.
Methods We analyzed seven microarray chips from the Gene Expression Omnibus (GEO) database including 135 lung cancer samples with miRNA, lncRNA, or mRNA to identify differentially expressed molecules in primary versus metastatic lung cancer. The clinical relevance of identified molecules was confirmed by examining clinical data from 32 patients with metastatic lung cancer in The Cancer Genome Atlas (TCGA) dataset. Co-expression and protein-protein-interaction (PPI) patterns were analyzed to identify candidate hub genes. Tumor immune infiltrate was analyzed to determine potential mechanisms of the hub genes.
Results We identified 15 miRNAs (miR-483-3p, miR-519-b-5p, miR-519c-5p, miR-586, miR-647, miR-875-3p, miR-137, miR-924, miR-922, miR-199b-5p, miR-610, miR-519c-3p, miR-548m, miR-384, miR-1289) and 6 target genes (SORBS1, GAB1, RPS6KA2, KIT, LRCH2, and PECAM1) with differential expression in primary versus metastatic disease. In addition, we identified 105 lncRNAs that were associated with metastatic lung cancer. The PPI network predicted 14 transcription factors that were likely to be involved the development of metastatic lung cancer, and analysis of the immune infiltrate showed that hub genes in the PPI network may alter the tumor immune microenvironment to drive metastatic progression.
Conclusions We identified key molecules that form a functional network to regulate the development and progression of metastatic lung cancer that may be promising new targets to treat this lethal disease.
A Systematic Review of Novel Therapies of Pulmonary Arterial Hypertension
Authors: Nabeh Omnia Azmy 
Published: February 2023

Abstract
Pulmonary arterial hypertension (PAH) is a progressive cureless disease, characterized by increased pulmonary vascular resistance and remodeling, with subsequent ventricular dilatation and failure. New therapeutic targets are being investigated for their potential roles in improving PAH patients’ symptoms and reversing pulmonary vascular pathology. We aimed to address the available knowledge from the published randomized control trials (RCTs) concerning the role of Rho kinase (ROCK) inhibitors, Bone morphogenetic protein 2 (BMP2) inhibitors, Estrogen inhibitors, and AMP- activated protein kinases (AMPK) activators on the PAH evaluation parameters. This systematic review (SR) was registered on PROSPERO (CDR42022340658) and followed the PRISMA guidelines. About 5092 records were screened from different database and registries and only 8 RCTs that met our inclusion criteria were included. The marked difference in the study designs and the variability of the selected outcome measurement tools among the studies made performing a meta-analysis impossible. Yet, the main findings of this SR are the powerful potential of the AMPK activator and the imminent anti-diabetic drug, metformin and the BMP2 inhibitor, sotatercept as promising PAH modifying therapies. There is a need for long-term studies to evaluate the effect of the ROCK inhibitor, fasudil and the estrogen aromatase inhibitor, anastrozole in PAH patients. Tacrolimus role in PAH is query. The discrepancy in the hemodynamic and clinical parameters necessitates defining cut values to predict improvement. The differences in the PAH etiologies render the judgment of the therapeutic potential of the tested drugs challenging.
Calf Deep Veins Are Safe And Feasible Accesses For The Endovascular Treatment Of Acute Lower Extremity Deep Vein Thrombosis
Authors: Zeng Xiande , Chen Wei , Min Xixi , Zhou Weimin , Qiu Jiehua 
Published: February 2023

Abstract
Purpose
This study was designed to assess the optimal access route for the endovascular treatment of acute lower extremity deep vein thrombosis.
Methods
This was a retrospective analysis of patients with acute lower extremity deep venous thrombosis who underwent endovascular treatment from February 2009 – December 2020. Patients underwent non-direct calf deep vein puncture (NDCDVP) from February 2009 - December 2011 and direct calf deep vein group (DCDVP) from January 2012 - December 2020. Catheter directed thrombolysis (CDT) was used to treat all patients in the NDCDVP group, whereas patients in the DCDVP group were treated with CDT or the AngioJet rheolytic thrombectomy system. In patients exhibiting iliac vein compression syndrome, the iliac vein was dilated and implanted with a stent. Technical success rates and perioperative complication rates were compared between these two treatment groups.
Results
The NDCDVP group included 83 patients (40 males, 43 females) with a mean age of 55 ± 16 years, while the DCDVP group included 487 patients (231 males. 256 females) with a mean age of 56 ± 15 years. No significant differences were observed between these groups with respect to any analyzed clinical characteristics. The technical success rates in the NDCDVP and DCDVP groups were 96.4% and 98.2%, respectively (P > 0.05). In the NDCDVP group, the sall saphenous vein(SSV)or great saphenous vein༈GSV༉were the most common access routes (77.1%, 64/83), whereas the anterior tibial vein was the most common access route in the DCDVP group (78.0%, 380/487), followed by the posterior tibial vein and peroneal vein (15.6% and 6.4%, respectively). Relative to the NDCDVP group, more patients in the DCDVP group underwent the removal of deep vein clots below the knee (7.2% [6/83] vs. 24.2% [118/487], P < 0.001). Moreover, relative to the NDCDVP group, significantly lower complication rates were evident in the DCDVP group (local infection: 10.8% vs. 0.4%, P < 0.001; local hematoma: 15.7% vs. 1.0%, P < 0.001). The position change rate was also significantly lower in the DCDVP group relative to the NDCDVP group (0% [0/487] vs. 60.2% [50/83], P < 0.001).
Conclusion
The calf deep veins represent a feasible and safe access route for the endovascular treatment of lower extremity deep vein thrombosis.
Comparison of in vitro antioxidant, antimicrobial and anticancer activity of Murraya koenigii leaf and stem extracts in ovarian cancer cells
Authors: Hajare Shubham , Patade Tanvi , Shrimangale Jayashri , Gurav Pradnya , Pathak Pranav , R.N. Kedar 
Published: February 2023

Abstract
Ovarian cancer (OC) is the most lethal gynecological cancer, due to its diagnosis at later stages III and IV. Drug resistance developed at later stages further decreases survival rates to 30% in ovarian cancer patients. Natural compounds isolated from plant extract can have promising potential in cancer treatment due to their minimal side effects. Murraya koenigii (MK) leaves commonly used in Indian cuisines, are known to have antimicrobial, antifungal, and antioxidant activity. However, very few studies show the effect of MK extracts on ovarian cancer. This study shows the comparison of phytochemical content, antioxidant, antimicrobial and cytotoxic activities of methanol, aqueous and toluene extracts of MK leaves and stems. These extracts showed anticancer activity on the ovarian teratocarcinoma cell line - PA1 via apoptosis and antiangiogenic effect on healthy chick embryos.
Status of self-medication and the relevant factors regarding drug efficacy and safety as important considerations among adolescents aged 12-18 in China: a cross-sectional study
Authors: Ge Pu , Liu Diyue , Li Xialei , Hong Wenying , Huang Mengjie , Zhu Lijun , Kaierdebieke Ayidana , Yu Wenbian , Qi Jiale , Pu Keping , Ling Rong , Sun Xinying , Wu Yibo , Feng Qiqin 
Published: February 2023

Abstract
Background: Self-medication is widely practiced among adolescents, and inappropriate self-medication can cause irreversible damage to adolescents' health. The attributes of over-the-counter medications influence adolescents' behavior when self-medicating.
Method: Multi-stage sampling was used to conduct a questionaire investigation in Mainland China in 2021. The questionnaire contains sociodemographic characteristics and several scales, the presence of self-medication behavior and the types of drugs they self-medicated, and important considerations when self-medicating. After a statistical description of the data, logistic regression were used to analyse the factors related to the types of drugs in self-medication as well as the consideration of drug efficacy and safety
Result: 1065 questionnaires were returned and 969 were valid, with an effective rate of 90.99%. The self-medication rate among Chinese aged 12-18 was 96.61%. 65.84% considered drug safety as an essential consideration, and 58.72% took drug efficacy as an important consideration. The multi-factor regression showed that those with better health care scores were more likely to consider drug efficacy as an essential factor(OR=1.554 P＜0.05);those with high family healthy lifestyle scores were more likely to focus on efficacy(OR=1.483 P<0.05). Family health status and monthly per capita household income were associated with whether respondents took drug safety as an important consideration. Those with higher per capita monthly household income (≥$641) were less inclined to consider safety as an essential factor(OR=0.544 P<0.001). Investigators with beller disease prevention were more likely to consider drug safety as an essential factor(OR=2.247 P<0.001);those with better family health resources were more likely to consider drug safety as an essential factor (OR=1.516 P＜0.05).
Conclusion: Self-medication is prevalent among 12-18-year-old adolescents in China. Demographic and sociological characteristics, health literacy, and family health status were associated with the likelihood that adolescents would focus on the safety and efficacy of medication when self-medicating. Health literacy and family health status were positively correlated with the likelihood of considering the efficacy and safety of drugs as important factors when self-medicating. Authorities should take measures to regulate adolescents' self-medication behaviour, strengthen health education for adolescents and their supervisors, and improve their health literacy to reduce the harm caused by inappropriate self-medication.
The relationship between the severity of coronary artery disease in angiography and the incidence of short-term major cardiovascular events of patients with Covid-19 and myocardial infarction: a cross-sectional study in Iran
Authors: Madadi Reza , Kalantari Zahra , Kalantari Soroor , Makoui Reza Hassanzadeh , Ahangar Hassan , Sepehr Gohari , Mahjani Mahsa , Mansouri Kamyar 
Published: February 2023

Abstract
Background
Coronary artery disease (CAD) has been frequently recognized as a risk factor for poor prognosis in COVID-19 patients. Syntax score is an invasive coronary angiographic-based tool used to determine the severity of CAD. In this study, we aim to investigate the prognostic significance of syntax score for mortality and morbidity among COVID-19 patients.
Methods
In this cross-sectional study, we have included patients with confirmed COVID-19 diagnosis who underwent percutaneous coronary intervention (PCI). Based on angiographic records, the CAD complexity was measured by Syntax score, and echocardiographic variables were documented. The laboratory data were obtained from the HISS database of the hospital. All patients were followed up one month after discharge for new cardiovascular events, rehospitalization, heart failure (HF), stent thrombosis, cerebrovascular accidents, and death.
Results
In one month, 108 patients were included in the study. The mean age was 64.8 ± 11.6, and 74% were male. The Cox regression model found no association between the Syntax score and the composite outcomes. In the univariate cox proportional HR model, MPV, LDH, and ESR were found to have predictive significance for in-hospital death. AKI was resulted to be significantly associated with rehospitalization in multivariate analysis.
Conclusion
The present study did not find a significant association between adverse outcomes and syntax score in COVID-19 patients referred for PCI. Acute kidney injury and duration of ICU stay was found to be the main factor predicting rehospitalization and HF. Future studies are needed to confirm these findings.
Assessing the gut microbiome and the influence of host genetics on a critically endangered primate, the Northern muriqui (Brachyteles hypoxanthus)
Authors: Sales Naiara Guimaraes , Kaizer Mariane da Cruz , Browett Samuel D. , Gabriel Sofia I. , McDevitt Allan D. 
Published: February 2023

Abstract

Background The Northern muriqui (Brachyteles hypoxanthus) is one of the world’s most critically endangered primates, currently represented by only ~ 1000 remaining mature individuals in the wild. The largest new world primate, this endemic herbivore plays an important role in seed dispersal in the Brazilian Atlantic Forest. However, due to several threats such as habitat loss and hunting, the species is in sharp decline and conservation actions are paramount to safeguarding its persistence. Analyses of gut microbiomes in wild populations can contribute to assessing the health status of the host species through the identification of microbial community changes which could lead to increased host vulnerability and disease. This can ultimately contribute to baseline knowledge towards improving conservation programmes and reintroduction efforts. Results In this study, we analysed the microbiome (using 16S rRNA metabarcoding) of faecal samples belonging to 53 uniquely genotyped individuals from three social groups located in a protected area (Caparaó National Park) aiming to provide the first assessment of the microbiome diversity and composition for this species. Our results showed the muriqui gut microbiome was predominantly composed of the phylaBacteroidetesandFirmicutes, with the dominant classes represented byBacteroidiaandClostridia. High similarity in both alpha and beta diversities was found for individuals from distinct groups, suggesting a lower environmental filtering effect on microbiota composition and a negligible geographical effect at the fine spatial scale analysed. Additionally, no significant effect of heterozygosity levels on microbiota diversity was recovered, but a significant influence of genetic distance on microbiota community structure and composition was demonstrated. Conclusions Here, we showed the importance of host genetics on the microbiome and highlighted that although the analysed populations are deemed isolated due to putative environmental barriers, they host a similar microbiome composition, with faecal microbiome stability possibly associated with host genetics, and the linked shared feeding strategies. Furthermore, the detailed wild Northern muriqui microbiome assessment herein included might be used for subsequent management and conservation actions considering future anthropogenic impact assessments on the species and animal relocations and/or reintroductions.
Ectopic Cushing’s syndrome: still much more to understand. A case report and a mini-review
Authors: Pernazza Matteo , Somma Chiara , Tsalouchos Aris , Gallo Pamela , Dattolo Pietro Claudio 
Published: February 2023

Abstract
Introduction-We describe the case of a patient Ectopic Cushing’s syndrome without founding any extrapituitary tumour secreting adrenocorticotropic hormone (ACTH).
In the literature there are only a few reported cases and generally the site of the tumor is subsequently identified. In addiction we performed a mini-review of the literature.
Case report-We report the case of a patient with lower extremity edema, weight gain, uncontrolled hypertension, hypokalemic metabolic alkalosis and hyperglycemia suffering from ACTH-dependent Cushing's syndrome. None of the instrumental examinations allowed us to locate the source of ACTH secretion. Despite a medical therapy with maximum dosage of steroidogenesis inhibitors a bilateral adrenalectomy was performed due to the development of a life-threatening hypercortisolism. After 12 months from surgery follow-up is still negative for ACTH-secreting tumour.
Conclusion- In a lot of cases Cushing’s syndrome is still nowadays not diagnosed although current advanced techniques. In occult Cushing’s syndrome bilateral adrenalectomy is the best therapy when a life-threatening hypercortisolism develops. The ACTH-secreting tumour could be occult for many years after the onset of symptoms. Follow-up in patients with occult ectopic Cushing’s syndrome undergoing bilateral adrenalectomy should be prolonged for life
Plasmonic characteristics of rhodium dual brokennanorings in UV-visible regime
Authors: Zhang Yue , Xiong Tao , Dong Dandan , Wang Wei , Sun Cheng 
Published: February 2023

Abstract
This work reports on the plasmonic properties of a symmetry-breaking system consisting of rhodium dual broken nanorings, in the ultraviolet-visibleregime. In the structure, two rhodium broken rings are located with a separation on the scale of nanometers. As the separation, the light polarization,and the relative orientation of the broken angles are respectively varied, theplasmonic scattering efficiency of the system is investigated, using the finite difference time domain method. Multiple plasmonic resonances are revealed,and the associated asymmetry-induced Fano-like lineshapes are fitted to amodel that employs multiple Fano lineshape functions. The resonance wave-lengths, the spectral widths, and the characteristic q values are determinedfrom the best fit parameters, and the plasmonic characteristics of the sys-tem are quantitatively probed. The results in this work may be beneficial indesigns of plasmonic devices that operates at ultraviolet-visible wavelengths.
Effects of Escherichia pollution and salinity on nutrient levels in submerged vegetated wetlands: Insights into benthic community stability and metabolisms
Authors: Chen Jia-yun , Wu Jian-yi , Xu Run-yang , Hua Zu-lin , Gu Li 
Published: February 2023

ABSTRACT
Inner coastal wetland ecosystems are generally eutrophic and are often exposed to both salinity stress and Escherichia coli pollution. However, the effects of these stressors on nutrient-cycling and microbial communities are under-researched. Here, we established a vegetated wetland ecosystem in a saline environment to understand the effects of E. coli pollution on nutrient removal and benthic microorganisms. The results show that E. coli significantly inhibited nutrient removal, especially total nitrogen (TN) and ammonium (78.89–84.98 and 3.45–44.65% were removed from the non-E. coli-treated and the E. coli-treated water, respectively). Compared with non-vegetated systems, archaeal community variations at both compositional and phylogenetic levels were weakened in vegetated systems (p &lt; 0.05). Among all the environmental factors, the ratios of PO43--P to total phosphorus and NO3--N to TN contributed the most to archaeal and bacterial community structural variations, respectively. E. coli pollution affected archaeal community succession more than bacteria (p &lt; 0.05). E. coli also weakened the trophic transferring efficiencies between Cyanobacteria and Myxobacteria (p &lt; 0.05). Metabolically, E. coli inhibited bacterial genetic metabolic pathways but made human infection more likely (p &lt; 0.05). Our findings provide new insights into aquatic ecological conservation and environmental management.
Hydrochemical characteristics and driving factors of travertine deposition in Huanglong, Sichuan, SW, China
Authors: Gao Wenhao , Zhang Jing , Zhang Weizhen , Sun Dong , Guo Jiawei , Zhao Songjiang , Zeng Ying , Liu Xinze 
Published: February 2023

ABSTRACT
The hydrochemical characteristics were analyzed by mathematical statistics, the hydrochemical types were analyzed by Piper three line diagram, and the sources and influencing factors of main ions in surface water were discussed by Gibbs diagram and ion correlation analysis. The results show that the TDS of surface water in the study area is 109–559 mg·L−1, and the average value is 318.67 mg·L−1; The pH value is 6.81–8.62, and the average value is 7.85. Most of them belong to weakly alkaline water. The surface water cation is mainly Ca2+ and Mg2+, the anion is mainly HCO3- and the hydrochemical type is HCO3–Ca. Through the correlation analysis of the main ions, it can be seen that TDS has a significant positive correlation with Na+, K+, Mg2+, Ca2+ and HCO3−, and these ions contribute to TDS. HCO3− has a significant correlation with Na+, K+ and Mg2+ and comes from carbonate rocks. According to the analysis of water–rock model, the hydrochemical genesis of surface water in the study area is mainly controlled by rock weathering, most ions are weathered and dissolved by carbonate rock and evaporated salt rock and a few cations are affected by water ion exchange.
Antioxidant and Antiangiogenic Effect of Homoleptic Palladium (II) Carboxamide Complex Loaded Chitosan Modified PLGA Nanoparticles: In vitro Evaluation and In vivo Chick Embryo Chorioallantoic Membrane (CAM) Assay
Authors: Öztürk A. Alper , Ünver Hakan , Kıyan H. Tuba 
Published: February 2023

Background:Angiogenesis is a natural and complex mechanism that is regulated by biomolecules formed by the body. Medicinal inorganic chemistry is increasing in popularity due to metal-based compounds, offering significant chances and possibilities for building novel anti-cancer medicines with promising anti-angiogenic effects.Objective:This study aimed to examine the successful results obtained from treatments with nanoparticle formulations of active drug substances.Methods:The nanoprecipitation/solvent displacement approach, with some changes, was used to make PLGA-based NPs.Results:The particle size obtained in the blank formulation was 82.4–473.9 nm, while the particle size in the API-loaded NPs was 193.2–678.0 nm. Among the formulations, NP-950-P, NP-390-P, and NP-350-CSP2 were found to have significant antioxidant potentials with IC50 values of 3.025, 5.198, and 7.4242 µg.mL-1, respectively, when compared to Vit C. According to the microscopic evaluations, NP-950-P (including Pd(PyCrbx)2Cl2 as 50 µg.pellet-1) and NP-950-CSP2 (including Pd(PyCrbx)2Cl2 as 50 µg.pellet-1) showed strong anti-angiogenic effect whereas the other NP formulations showed weak anti-angiogenic effect when compared with the positive control (±)-Thalidomide at the concentration of 50 µg.pellet-1.Conclusion:When the results were examined, it was found that nanoscale drug carrier systems were prepared, and high antioxidant activity and anti-angiogenesis activity were detected, especially in nanoparticles prepared with 950. As per our knowledge, it is the first study in this field that will bring a new perspective to cancer treatment.