bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disinfectant, Soap or Probiotic Cleaning? Surface Microbiome Diversity and Bioﬁlm Competitive Exclusion
Authors: Tolmay Janke , Tucker Keira , Wolfaardt Gideon 
Published: February 2023

<p>This study extends probiotic cleaning research to a built environment. Through an eight-month cleaning trial, we compared the effect of three cleaning products (disinfectant, plain soap, and a probiotic cleaner containing a patented Bacillus spore consortium), and tap water as the control, on the resident microbiome of three common hospital surfaces (linoleum, ceramic, and stainless steel). Pathogens, Escherichia coli and Staphylococcus aureus, were deposited and desiccated, and competitive exclusion was assessed for each microbiome. Cell survival was shown to be an incomplete tool for measuring microbial competitive exclusion. Biofilm competition offered a fuller understanding of competitive dynamics. A test for culturable cell survival showed that both plain soap and probiotic cleaner regimes established a surface microbiome that outcompeted the two pathogens. A different picture emerged when observing biofilms with a deposited and desiccated GFP-labeled pathogen, Pseudomonas aeruginosa. Competitive exclusion was again demonstrated. On surfaces cleaned with disinfectant the pathogen outcompeted the microbiomes. On surfaces cleaned with plain soap, the microbiomes outcompeted the pathogen. However, on surfaces cleaned with probiotic cleaner, despite the exponentially higher surface microbial loads, the microbiome did not completely outcompete the pathogen. Thus, the standard culturable cell test for survival on a surface confirmed the competitive advantage that is typically reported for probiotic cleaners. However, observation of competition in biofilms showed that the more diverse microbiome (according to alpha and beta indices) established on a surface cleaned with plain soap had a better competitive advantage than the monoculture established by the probiotic cleaner. Therefore, microbial diversity appears to be as critical to the competitive exclusion principle as cell numbers. The study showed that both plain soap and probiotic cleaner fostered competitive exclusion far more effectively than disinfectant. Probiotic cleaners with microbial diversity could be worth considering for hospital cleaning. </p>
GRNTI 2022 master version. (Part 1. Developing thematic and structural organization of subject heading nodes)
Authors: Shrayberg Ya. L. , Dmitrieva E. Y. , Pronina T. A. , Smyslova I. S. , Terekhova E. S. 
Published: February 2023

The authors introduce the findings of their research on updating and improving the existing master version of the State Rubricator of Sci-tech Information (GRNTI) version 2021 and building the General Classification Schedule of the master GRNTI 2022 version. They define the main functions of GRNTI as the standard classification of the State System of Sci-tech Information. The functional efficiency depends on the compliance of the Rubricator’s subject and structural organization to the current status and trends of scientific knowledge and technologies. The main stages of the accomplished studies are described: comparative intellectual analysis of subject scope and hierarchy of GRNTI classes and thematic description of sections within the Plan of Fundamental and Thematic Scientific Research for 2021–2030; submitting proposals for GRNTI by researchers of relevant research institutes and STI agencies; analyzing and content editing of GRNTI proposals, eliminating concept duplication, and updating links and references. The authors include the statistics of changes distribution in GRNTI 2021 by types: changed subject headings, new headings introduction, and elimination of the subject headings. Statistical indicators are analyzed to characterize GRNTI subject and structural organization, as well as its thematic classes (2nd and 3rd level headings).
SELF EFFICACY SISWA SMA SEDERAJAT TERHADAP PEMAHAMAN KONSEP SPLDV SELAMA PEMBELAJARAN DARING
Authors: Rantayu Riham Fahira , Rahayu Aninda Aulia , Ibrahim  
Published: February 2023

Penelitian ini bertujuan untuk mengetahui hubungan antara self efficacy terhadap pemahaman konsep matematika materi SPLDV pada siswa SMA sederajat selama pembelajaran daring. Jenis penelitian yang digunakan adalah kuantitatif dengan pendekatan metode survei. Instrumen yang digunakan ada 2 angket yaitu angket self efficacy yang menggunakan skala likert dan angket pemahaman konsep yang berupa skala ordinal 1-5. Subyek penelitian adalah 30 orang siswa SMA sederajat. Analisis data yang digunakan adalah uji normalitas, uji linearitas dan uji regresi sederhana. Hasil penelitian diperoleh bahwa adanya hubungan antara self efficacy dengan kemampuan pemahaman konsep siswa selama pembelajaran matematika daring pada materi SPLDV dan ditunjukkan bahwa hubungan antara keduanya tidak saling mempengaruhi dikarenakan nilai korelasinya kecil.
PENGARUH IKLAN SHOPEE PAYLATER TERHADAP PEMANFAATAN FITUR CICILAN OLEH MAHASISWA
Authors: Baral Muhammad Mubaral , Widiastuti Wahyu , Muzni Nurlianti 
Published: February 2023

ABSTRAK
Tujuan penelitian ini adalah untuk mengetahui apakah ada pengaruh dari iklan yang disampaikan oleh Shopee paylater terhadap pilihan pemanfaatan fitur cicilan oleh Mahasiswa Ilmu Komunikasi Angkatan 2018-2020 Universitas Bengkulu. Jenis pendekatan yang digunakan ialah pendekatan penelitian kuantitatif, serta untuk mengetahui seberapa besar pengaruh yang ditimbulkan. Teori yang digunakan dalam penelitian ini ialah teori AIDDA (Attention, Interest, Decision, Desire, Dan Action). Teknik pengumpulan data dilakukan dengan menyebar kuesioner kepada 75 mahasiswa. Teknik pengambilan sampel yang digunakan adalah stratified random sampling. Data pengujian hipotesis dilakukan dengan uji regresi linier sederhana melalui program SPSS 25.0. Hasil penelitian menunjukkan bahwa variabel Iklan Shopee Paylater berpengaruh terhadap pemanfaatan fitur cicilan pada mahasiswa ilmu komunikasi Universitas Bengkulu dengan koefisien determinasi (R Square) diperoleh nilai sebesar 0,222 yang menunjukkan bahwa 22% Iklan yang disampaikan oleh Shopee paylater sedangkan sisanya, 78% dipengaruhi oleh variabel-variabel lainnya yang tidak diteliti dalam penelitian ini.

Kata Kunci : Iklan, ShopeePaylater, Fitur Cicilan, Mahasiswa

THE EFFECT OF SHOPEE PAYLATER ADVERTISING OF THE INSTALLMENT FEATURE BY STUDENTS

ABSTRACT
The purpose of this study is to find out whether there is an influence of the advertisements submitted by Shopee paylater on the choice of utilizing the installment feature by Communication Science Students class of 2018-2020, Bengkulu University. The type of approach used is a quantitative research approach, as well as to find out how much influence it has. The theory used in this study is the AIDDA theory (Attention, Interest, Decision, Desire, And Action). The data collection technique was carried out by distributing questionnaires to 75 students. The sampling technique used is stratified random sampling. Hypothesis testing data were performed with a simple linear regression test through the SPSS 25.0 program. The results showed that the Shopee Paylater Advertising variable affected the use of the installment feature in Bengkulu University communication science students with a coefficient of determination (R Square) obtained a value of 0.222 which showed that 22% of the advertisements submitted by Shopee paylater while the rest, 78% were influenced by other variables that were not studied in this study.

Keywords : Advertising, ShopeePaylater, Installment Feature, Student
Incidence and risk factors of postoperative nausea and vomiting following laparoscopic sleeve gastrectomy and its relationship with Helicobacter pylori: A propensity score matching analysis
Authors: Song Yali , Zhu Jie , Dong Zhiyong , Wang Cunchuan , Xiao Jia , Yang Wah 
Published: February 2023

BackgroundPostoperative nausea and vomiting (PONV) are common after laparoscopic sleeve gastrectomy (LSG), affecting patient satisfaction and postoperative recovery. The purpose of this study was to investigate the incidence and severity of PONV after LSG and the relationship between Helicobacter pylori (HP) and PONV.MethodsPatients undergoing LSG in our center from June 1, 2018, to May 31, 2022, were divided into HP-positive and HP-negative groups for retrospective analysis. The independent risk factors of PONV were determined by univariate and binary logistic regression analysis using a 1:1 propensity score matching (PSM) method.ResultsA total of 656 patients was enrolled, and 193 pairs of HP-positive and negative groups were matched after PSM. Both groups of patients had similar clinical features and surgical procedures. PONV occurred in 232 patients (60.1%) after LSG, and the incidence of PONV in HP-positive patients was 61.10%. The incidence and severity of PONV were statistically similar in both groups (P=0.815). Multivariate analysis showed that the female sex (OR=1.644, P=0.042), postoperative pain (OR=2.203, P=0.001) and use of postoperative opioid (OR=2.229, P=0.000) were independent risk factors for PONV after LSG, whereas T2DM (OR=0.510, P=0.009) and OSAS (OR=0.545, P=0.008) independently reduced the incidence rate of PONV. There was no difference either in smoking (P=0.255) or alcohol drinking (P=0.801). HP infection did not affect PONV (P=0.678).ConclusionsThe incidence of PONV following LSG was relatively high. Female sex, postoperative pain and use of postoperative opioid predicted a higher incidence of PONV. Patients with T2DM and OSAS were less likely to have PONV. There was no clear association between HP infection and PONV after LSG.
Evolution of antibody profiles against SARS-CoV-2 in experienced and naïve vaccinated elderly people
Authors: Sanz-Muñoz Iván , López-Mongil Rosa , Sánchez-Martínez Javier , Sánchez-de Prada Laura , González Marta Domínguez-Gil , Pérez-SanJose Diana , Rojo-Rello Silvia , Hernán-García Cristina , Fernández-Espinilla Virginia , de Lejarazu-Leonardo Raúl Ortiz , Castrodeza-Sanz Javier , Eiros José María 
Published: February 2023

IntroductionThe third dose of the COVID-19 vaccine is especially necessary in people over 65 years of age due to their lower immune response.MethodsWe designed a multicentre, prospective observational study including 98 people ≤65 years old who lived in two nursing homes in Valladolid, Spain. One of the groups had previous experience with SARS-CoV-2 (n=68;69.4%) and the other was naïve (n=30;30.6%). We evaluated the response to the three doses of the Comirnaty vaccine and the dynamics of antibodies during 5 consecutive serum samplings: 2 after the first two doses of vaccination, one three months after the first dose, another at 6 months and the last one month after the third dose. IgG antibodies against SARS-CoV-2 S1, RBD and N antigens were analysed.ResultsBoth groups increased the level of Abs against S1 and RBD, but the experienced group showed a 130-fold higher humoral response due to hybrid immunisation (infection+vaccination). The response to vaccination with Comirnaty against COVID-19 was higher in those ≤65 years with previous experience than those who were naïve. However, the amount of antibodies against S1 and RBD equalised at 6 months. After the third dose, both groups raised the amount of antibodies to a similar level. The reinfections suggested by the analysis of antibodies against N were frequent in both groups.DiscussionThe third dose showed a clear benefit for elderly people, with the reinforcement of the antibody levels after the decline suffered after six months of the first two doses.
Impact of local iron enrichment on the small benthic biota in the deep Arctic Ocean
Authors: Soltwedel Thomas , Rapp Josephine Z. , Hasemann Christiane 
Published: February 2023

This study assesses the impact of local iron enrichment on the small benthic biota (bacteria, meiofauna) at the deep seafloor. To evaluate the hypothesis that abundance, distribution, and diversity of the small benthic biota varies in relation to a local input of structural steel at the seabed, we analyzed sediment samples and the associated infauna along a short transect (~1.5 m in length) with increasing distance to an iron source, i.e., corroding steel weights (30 cm in length and width, and 6 cm in height) of a free-falling observational platform (bottom-lander), lying on the seafloor for approximately seven years. Bacterial and meiofaunal densities and biomasses in iron-enriched sediments were significantly lower than those in unaffected sediments. Moreover, bacterial and nematode community structure between iron-enriched sediments and unaffected sediments differed strongly; taxonomic richness as well as diversity was lowest closest to the iron source. The presence of iron fostered the establishment of specialized iron oxidizers and other chemolithoautotrophic bacterial members, which were rare or absent in the unaffected sediments, within which opportunistic heterotrophs predominated. Nematodes comprised &gt;90% of the total metazoan meiofauna and were therefore studied in more detail. A total of 26 genera from 16 families occurred in iron-enriched sediments (three genera were found exclusively in these sediments), while 65 genera from 27 families occurred in the unaffected sediments (39 genera and 12 families were found exclusively in these sediments). Nematode genera number (S), estimated genera richness (EG(51)) and heterogeneity (H’(log2)) were significantly lower in iron-enriched sediments than in unaffected sediments. Our results confirm that the local enrichment of deep-sea sediments by metallic and corroding structures (e.g., by ship hulls, containers, scientific equipment) strongly affects the diversity of the small benthic biota at short distances from these sources.
“CORE” a new assay for rapid identification of Klebsiella pneumoniae COlistin REsistant strains by MALDI-TOF MS in positive-ion mode
Authors: Foglietta Gianluca , De Carolis Elena , Mattana Giordana , Onori Manuela , Agosta Marilena , Niccolai Claudia , Di Pilato Vincenzo , Rossolini Gian Maria , Sanguinetti Maurizio , Perno Carlo Federico , Bernaschi Paola 
Published: February 2023

Due to the global spread of pan resistant organisms, colistin is actually considered as one of the last resort antibiotics against MDR and XDR bacterial infections. The emergence of colistin resistant strains has been observed worldwide in Gram-negative bacteria, such as Enterobacteriaceae and especially in K. pneumoniae, in association with increased morbidity and mortality. This landscape implies the exploration of novel assays able to target colistin resistant strains rapidly.In this study, we developed and evaluated a new MALDI-TOF MS assay in positive-ion mode that allows quantitative or qualitative discrimination between colistin susceptible (18) or resistant (32) K. pneumoniae strains in 3 h by using the “Autof MS 1000” mass spectrometer. The proposed assay, if integrated in the diagnostic workflow, may be of help for the antimicrobial stewardship and the control of the spread of K. pneumoniae colistin resistant isolates in hospital settings.
Efficacy and tolerability of oral gastrodin for medication overuse headache (EASTERN): Study protocol for a multicenter randomized double-blind placebo-controlled trial
Authors: Kong Fanyi , Buse Dawn C. , Geng Jia , Xu Jingjing , Liu Hanxiang , Ma Shu 
Published: February 2023

BackgroundProphylactic medication in clinical detoxification programs for the treatment of medication overuse headache is still debated. Gastrodin, a main bioactive constituent of Rhizoma Gastrodiae, has been applied clinically to treat primary headache for more than 30 years in China due to its potential analgesic and anti-migraine mechanisms. However, clinical evidence supporting its routing use in MOH is insufficient. The present study aims to investigate the efficacy and tolerability of oral gastrodin in medication overuse headache.MethodsA multicenter, randomized, double-blind, parallel, placebo-controlled trial will be performed. A target sample size of 186 patients who fulfill the International Classification of Headache Disorders 3rd version (ICHD-3) criteria for MOH will be recruited and screened during a baseline screening period of 28 days before being randomly assigned to either the gastrodin or placebo group at a ratio of 1:1. Enrolled patients will be assessed for each 4 weeks during the 12-weeks double-blind phase and followed up at week 24. The primary endpoint is mean change in monthly headache day frequency. Secondary endpoints will be the proportion of remitted MOH, change in headache pain intensity, headache impact test (HIT-6) score, 50% responder rate, treatment failure, monthly acute medication intake days, and Short Form 36-Item Health Survey (SF-36) score. Tolerability will be assessed by drop-out rates though safety monitoring during treatment.DiscussionThe findings of the present study may help to provide new evidence on gastrodin as a prophylaxis treatment with both efficacy and high tolerability for the treatment of MOH.Clinical trail registrationChinese Clinical Trail Registry (ChiCTR2200063719), Protocol Version 1.1, May, 09, 2022.
Case report: A case of acute disseminated encephalomyelitis after SARS-CoV-2 infection in pediatric patients
Authors: Cautilli Francesca , Feleppa Mariavittoria , Valeriani Massimiliano , Papetti Laura , Monte Gabriele , Midulla Fabio , Spalice Alberto 
Published: February 2023

IntroductionSince the beginning, there has been enough evidence about the multi-systematic involvement of the coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Recent observations have revealed that, together with others, typical neurological manifestations are also associated with COVID-19 infection. In the first 2 years, children accounted for a few percent of cases, but with the emergence of the Omicron variant, the number of cases in the pediatric population has increased. It has been described that ~5% of the affected population suffered from severe neurological complications, such as seizure, coma, encephalitis, demyelinating disorders, and aseptic meningitis. Acute disseminated encephalomyelitis (ADEM) is an inflammatory demyelinating disease of the central nervous system. Typically, it presents in childhood and occurs 1 or 2 weeks after infection or vaccination.Case presentationWe present the case of a 12-year-old boy who developed ADEM, 10 days after an asymptomatic SARS-CoV-2 infection. Neurological symptoms began with headache, fever, irritability, paraplegia, and loss of sensitivity from the T1 level. The diagnosis of ADEM was confirmed by the typical signs found on brain MRI, whereas spinal cord MRI showed signs of transverse myelitis. The cerebrospinal fluid (CSF) testing excluded infections and did not reveal oligoclonal antibody bands (anti-MOG-negative and anti-AQP-negative). High-dose steroids (30 mg/kg/day) and IVIG (2 g/kg) were administered to the patient without any clinical improvement. The patient received a cycle of plasma exchange therapy, followed by rituximab infusion, with partial improvement. After 3 months, the magnetic resonance imaging (MRI) results demonstrated radiological improvement in accordance with the ADEM diagnosis.ConclusionThis clinical case confirms that SARS-CoV-2 infections are increasingly implicated in severe neurological consequences in both adult and pediatric patients. While the most frequent complications that were reported in children included headache, altered mental status, and encephalopathy, ~5% of the individuals suffered from severe neurological complications, leading to lifelong sequelae. All physicians must be aware of these data and detect neurological signs of severe (or not) complications that require a specific follow-up and treatment.
Delivering acute stroke care in a middle-income country. The Mexican model: “ResISSSTE Cerebro”
Authors: Bonifacio-Delgadillo Dulce María , Castellanos-Pedroza Enrique , Martínez-Guerra Bernardo Alfonso , Sánchez-Martínez Claudia Marisol , Marquez-Romero Juan Manuel 
Published: February 2023

IntroductionFounded in 2019, the “ResISSSTE Cerebro” program is the first and only stroke network within the Mexican public health system. One advanced stroke center (ASC) and seven essential stroke centers (ESC) provide acute stroke (AS) care through a modified hub-and-spoke model. This study describes the workflow, metrics, and outcomes in AS obtained during the program's third year of operation.Materials and methodsParticipants were adult beneficiaries of the ISSSTE health system in Mexico City with acute focal neurological deficit within 24 h of symptom onset. Initial evaluation could occur at any facility, but the stroke team at the ASC took all decisions regarding treatment and transfers of patients. Registered variables included demographics, stroke risk factors, AS treatment workflow time points, and clinical outcome measures.ResultsWe analyzed data from 236 patients, 104 (44.3%) men with a median age of 71 years. Sixty percent of the patients were initially evaluated at the ESC, and 122 (85.9%) were transferred to the ASC. The median transfer time was 123 min. The most common risk factor was hypertension (73.6%). Stroke subtypes were ischemic (86.0%) and hemorrhagic (14.0%). Median times for onset-to-door, door-to-imaging, door-to-needle, and door-to-groin were: 135.5, 37.0, 76.0, and 151.5 min, respectively. The rate of intravenous thrombolysis was 35%. Large vessel occlusion was present in 63 patients, from whom 44% received endovascular therapy; 71.4% achieved early clinical improvement (median NIHSS reduction of 11 points). Treatment-associated morbimortality was 3.4%.ConclusionWith the implementation of a modified hub-and-spoke model, this study shows that delivery of AS care in low- and middle-income countries is feasible and achieves good clinical outcomes.