bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detoksifikasi Aluminium dan Ketersediaan Fosforus dalam Tanah Masam Melalui Aplikasi Bahan Organik
Authors: Indriyati Lilik Tri , Nugroho Budi , Hazra Fahrizal 
Published: January 2023

Organic matter that is usually quite widely available around farmers' land can be one of the solutions in overcoming the problem of soil acidification and nutrient deficiencies in tropical highly weathered soils. However, one of the main problems in utilizing such organic matter on acidic soils is the diversity of its chemical composition, which affects the decomposition of the organic matter. In this study, a mixture of soil with five types of organic matter was incubated, namely cow manure, chicken manure, rice straw, legume plant residue, and their combination, with doses equivalent to 0, 7.5 and 15 tons/ha. Mixtures of soil with organic matter were incubated for 120 days under conditions of field capacity moisture. The aim was to evaluate the amelioration effect of the organic matter on soil acidification (exchangeable-Al and soil pH), available P, and phosphatase activity. The application of organic matter type and dose has a marked effect on decreasing the content of exchangeable Al and increasing soil pH, P-available, and phosphatase activity. Chicken manure application showed the best effect in lowering soil exchangeable-Al and increasing pH, available-P, and phosphatase activity.

Keywords: amelioration, manure, phosphatase
Skenario Konservasi Tanah pada Perkebunan Sawit sebagai Upaya Mitigasi Limpasan Permukaan pada DAS Arui, Kabupaten Manokwari, Papua Barat
Authors: Mahmud Mahmud 
Published: January 2023

Indonesia, a country with high forest and land potential for economic growth, still relies on utilizing these two potentials. This study aims to create a conservation scenario in palm oil plantations that are expected to be able to mitigate surface runoff. The data collected include hydrology, land, area of oil palm plantations, and topography, which were analyzed based on threats and potential conservation that can be applied. Conservation scenarios that need to be carried out are: deadlock alley ditch (dad), mixed tree and palm oil plantations, planting oil palm without land cultivation, and immersing mulch. The simulation results of the dad design, if it rains heavily, all of the rainwater in the palm oil fields can be accommodated without a runoff. Mixed tree and oil palm plants are expected to maintain the availability of nutrients, increase the income of oil palm smallholders, and have a good impact on soil fertility. Planting oil palm without land cultivation is expected to reduce surface runoff, increase water infiltration into the soil, and expand water catchment areas. The immersion of palm mulch is expected to help retain soil moisture, add nutrients, enhance the soil's ability to absorb water, and prevent erosion. Conservation scenarios in the long term, such as dad, mixed tree and oil palm plantations, oil plantations without land cultivation, and mulch immersion, are expected to be able to mitigate flooding.

Keywords: Arui watershed, conservation scenario, mitigation, oil palm, surface runoff
Keanekaragaman Undur-Undur Laut (Anomura: Hippidae) Dari Kepulauan Banda
Authors: Safrudin Nurulvadini , Suman Ali , Hanim Nisfa , Farajallah Achmad 
Published: January 2023

Mole crabs are part of the superfamily Hippoidea and members of the family Hippidae Latreille,1825, which inhabit sandy beaches, particularly the swash zone. This condition is commonly found in the Banda Islands. The purpose of this research was to determine the species diversity of the genus Hippa Fabricius, 1787 in the Banda Islands. We identified the species by its morphological characters. Sample collection was done by purposive sampling method. Based on the study's findings, four of the five previously reported species in Indonesia were identified. Hippa ovalis (A. Milne-Edwards, 1862), H. adaactyla Fabricius, 1787, H. marmorata (Hombron & Jacquinot, 1846), and H. celaeno (De Man, 1896) were the species obtained. Hippa cf. ovalis and Hippa cf. marmorata are both presumed to be new species. This study includes descriptions of the two species.

Keywords: distribution, Hippidae, Hippa, intertidal, sandy substrate
MARKETING STRATEGY OF GHARAR BUSINESS SCHEMES: Mystery Box on E-Commerce Shopee Platform
Authors: Febriandika Nur Rizqi , Tsany Nabila Putri , Sriplod Tipmanee 
Published: January 2023

ABSTRACT
Mystery box sale and purchase have developed into a fraudulent area, the prices and goods obtained are not appropriate, so consumers feel at a loss. This study aims to determine the marketing strategy and sales mechanism of the mystery box. The research method used in this research is descriptive qualitative with the type of research used in this study being observation by examining mystery box products in Shopee. Based on the results of the analysis carried out on the Shopee application, two types of keywords were found. The first keyword is the Mystery Box and the second keyword is the "Misteri Box" both have different product categories. There are ±27,000 products entitled mystery box and ±14,678 products with the name mystery box. So, it can be concluded that most of the sellers use titles with the keyword "mystery box". Mystery box on the Shopee site can be categorized as a marketing strategy that uses marketing segmentation without differentiating the market (Undifferentiated Marketing) and targeting in the form of full market coverage/mass market targeting.

ABSTRAK
Jual beli mystery box berkembang menjadi sebuah lahan penipuan, harga dan barang yang didapat tidak sesuai sehingga konsumen merasa rugi. Penelitian ini bertujuan untuk mengetahui strategi marketing dan mekanisme penjualan dari mystery box. Metode penelitian yang digunakan dalam penelitian ini adalah kualitatif deskriptif melalui pendekatan observasi pada produk mystery box yang ada di Shopee. Bedasarkan dari hasil analisis yang dilakukan pada aplikasi Shopee ditemukan dua jenis kata kunci, pertama adalah “Mystery box” dan kata kunci kedua adalah “Misteri Box”. Keduanya memiliki kategori produk berbeda. Hasil penelitian menunjukkan bahwa terdapat 27.000 produk yang muncul jika menggunakan kata kunci “mystery box” dan terdapat terdapat 7.000 produk jika menggunakan kata kunci “misteri Box”. Sehingga, dapat disimpulkan jika kebanyakan dari penjual lebih banyak menggunakan judul dengan kata kunci “mystery box”. Strategi pemasaran produk mystery box di Shopee dapat dikategorikan sebagai strategi marketing yang menggunakan segmentasi pemasaran tanpa membedakan pasar (Undifferentiated Marketing) dan targeting berupa cakupan pasar penuh (full market coverage/mass market targeting).
Molecular Identification of Elvers (<I>Anguilla</I> spp.) from River Estuaries in Central Java, Indonesia Using DNA Barcoding Based on <I>mt</I>DNA CO1 Sequences
Authors: Falah Iffan Nadhif , Adharini Ratih Ida , Ratnawati Susana Endah 
Published: January 2023

Highlight Research

The elvers of Anguilla were identified through the morphological and molecular markers
The result of morphological and molecular identification is different
The molecular identification helps to confirm the morphological identification
Based on this study molecular identification of the elvers of Anguilla found in river estuaries of Central Java is Anguilla bicolor bicolor

Abstract
An inventory of the presence and diversity of Anguilla spp. needs to be carried out as a basis for sustainable resource management. Conventional techniques based on morphological characteristics—are often less effective considering the morphological characteristics of Anguilla spp. are very identical and not so many can be observed, especially at the young eel stage. DNA-based molecular identification can be a way to determine diversity and phylogeny for conservation and inventory purposes. This research aimed to determine the diversity and phylogeny of Anguilla spp. obtained at the estuaries of Serayu River in Cilacap, Luk Ulo River in Kebumen, and Jali River in Purworejo in Central Java, Indonesia. A total of 10 samples were taken from each research location were analyzed morphologically, then it is known that there are 4 morphologically different samples at each research location so that a total of 12 samples were taken for molecular analysis. MEGA was used to construct the phylogenetic trees via Neighbour Joining (NJ) algorithms using the Kimura 2-parameter model with uniform rates and obtained by 1000 bootstraps replication. There are four species of Anguilla spp. identified morphologically, namely Anguilla bicolor bicolor, Anguilla bicolor pacifica, Anguilla obscura, and Anguilla australis. The species of Anguilla spp. that is molecularly identified is Anguilla bicolor bicolor with an identity percentage of > 98%. All samples are also known to be related to Anguilla bicolor bicolor identified in other countries.
Prevalence and Risk Factors of GERD among Stable COPD Patients
Authors: Hafiz Muhammad , Yunus Faisal , Suryamin Maulana , Alatas Mohammad Fahmi , Wibowo Adityo 
Published: January 2023

Introduction: Gastroesophageal reflux disease (GERD) is one of the most common causes of a chronic cough and is a potential risk factor for chronic obstructive pulmonary disease (COPD) exacerbation. This study aimed to determine the prevalence of GERD in COPD patients.
Methods: This was a cross-sectional study involving 40 stable COPD patients recruited from outpatient asthma and COPD clinics at Persahabatan National Respiratory Referral Hospital, Jakarta, from May to November 2018. COPD was defined as having a ratio of post-bronchodilator FEV1/FVC < 0.7 and no abnormality on a chest X-ray except emphysematous. Diagnosis of GERD was based on oesophageal mucosal lining break surrounding the distal esophageal sphincter through esophagogastroduodenoscopy (EGD). Asthma patients with known esophageal diseases such as cancer, achalasia, and active peptic ulcer and patients who had used proton pump inhibitors in the last 15 days were excluded.
Results: Patients were divided into GERD (+) (16/40, 40%) and GERD (-) (24/40, 60%). Subjects were predominantly elderly (25/40, 62.5%) and had a smoking history (36/40, 90%). Exacerbation and COPD assessment test (CAT) score was significantly associated with GERD (p < 0.05). No significant difference regarding age, sex, Brinkman Index, lung function, and body mass index (BMI) was found between groups. However, the GERD (+) group showed a slightly higher BMI and more severe airflow obstruction.
Conclusion: The prevalence of GERD in COPD patients found in this study was as high as 40%. A cohort study and preventive strategy of GERD in COPD should be studied further.
Final Condition of HIV Patients Infected with COVID-19 after Receiving Hospital Treatment: A Systematic Review
Authors: Hidayat Elin , Bakar Abu , Nursalam Nursalam , Indarwati Retno , Maria Herminia 
Published: January 2023

Introduction: Coronavirus disease 2019 (COVID-19) can attack anyone, including those who have human immunodeficiency virus (HIV) due to low immune systems. This study aimed to evaluate the final condition of people living with human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) (PLWHA) who received treatment at the hospital.
Methods: A literature investigation of the last three years (2020-2022) were inquired. Electronic databases (Science Direct, Scopus, ProQuest) with case series or case report designs were examined. The titles, abstracts, and full texts were screened to obtain relevant studies. Data tabulation and narrative analysis were conducted, resulting in 14 studies that met the inclusion criteria. Most of them were case series and case report methods. Each study had different numbers of patients and criteria discussing COVID-19 infection in HIV patients.
Results: A total of 14 reviewed studies revealed that HIV patients with COVID-19 could recover from the infection if proper treatments were performed. Some articles also stated that self-isolation at home can manage HIV-infected patients who are not yet severe. In the case of patients with severe infections, intensive care is performed using supplemental oxygen or a ventilator to prevent respiratory failure or acute respiratory distress.
Conclusion: COVID-19 can worsen the condition of patients with HIV if not appropriately treated. Otherwise, if the proper treatment is properly performed, it can cure the infection.
Pathogenetic aspects of the development of psoriatic arthritis in people with generalized chronic periodontitis
Authors: Poryadin G. V. , Zakhvatov A. N. , Parshina A. Yu. 
Published: January 2023

The pathogenetic mechanisms of progression of chronic periodontitis and psoriatic arthritis have common components in immune and inflammatory responses.The pathogenesis of chronic periodontitis involves interaction of microbial and immunological components. As a chronic immune-mediated inflammatory disease and a consequence of an infectious trigger that originally affects gingival soft tissue, periodontitis is typically characterized by periodontal destruction and damage to adjacent connective tissues. Neutrophils contribute to the development of periodontitis and participate in its progression by recruiting T helper 17 cells and stimulating synthesis of the receptor activator of the nuclear factor kappa-β ligand (RANKL), contributing to bone resorption.Macrophages as producers of proinflammatory cytokines (interleukin (IL)-1β, IL-6, IL-22, IL-23, tumor necrosis factor (TNF)), free radicals, and matrix metalloproteinases contribute to the chronic course of the disease. Tissue destruction results in generation of reactive oxygen species by neutrophils, which, against the background of a decrease in the antioxidant potential, leads to development of oxidative stress. These processes together lead to tooth mobility, formation of periodontal pockets, and bone resorption.The key factors in the formation of psoriatic arthritis against the background of periodontitis are overproduction of proinflammatory cytokines in target tissues (skin, joints, gingival microflora) and development of an excessive systemic immune response to the microbiota inhabiting the epithelial and periodontal tissues. A statistically confirmed correlation of the progression of periodontal destruction with the presence of psoriatic arthritis proves the significance of the effects of inflammation as a background for the progression of a comorbidity. Increased IL-17 synthesis plays a crucial role in the development of immune responses of pathological bone remodeling and bone resorption in periodontitis and psoriatic arthritis.
Development and characterization of patient-derived xenograft models of colorectal cancer for testing new pharmacological substances
Authors: Goncharova A. S. , Kolesnikov E. N. , Egorov G. Yu. , Maksimov A. Yu. , Shevchenko A. N. , Nepomnyashchaya E. M. , Gvaldin D. Yu. , Kurbanova L. Z. , Khodakova D. V. , Kit S. O. , Kaymakchi O. Yu. , Snezhko A. V. 
Published: January 2023

The aim of the study was to create a patient-derived xenograft (PDX) model of human colorectal cancer and to determine its histologic and molecular characteristics, such as the status of KRAS, NRAS, and BRAF genes and the presence of microsatellite instability.Materials and methods. First generation xenograft models in vivo were created using tumors from patients with colorectal cancer (n = 4) and immunodeficient Balb/c Nude mice (n = 20); second, third, and fourth generation models were created in the same mouse line (n = 3 for each generation). A caliper was used to measure subcutaneous xenografts; their size was calculated by the ellipsoid formula. Cryopreservation involved immersing the samples in a freezing medium (80% RPMI 1640, 10% fetal bovine serum, 10% dimethyl sulfoxide (DMSO)) and storing them at –80 °C. The histologic analysis was performed according to the standard technique (preparation of paraffin blocks and staining of microsections with hematoxylin and eosin). Mutations in the KRAS, NRAS, and BRAF genes were determined by direct Sanger sequencing; microsatellite instability was determined by the fragment analysis at five loci: Bat-25, Bat-26, NR21, NR24, and NR27.Results. Stable, transplantable xenografts of colorectal cancer were obtained from two out of four patients. The average waiting time from the implantation to the growth of the first generation xenograft was 28 days. The latency phase after cryopreservation was comparable to that at the creation of the first generation PDX model. The model reproduced the histotype, grade and mutational status of the KRAS, NRAS, and BRAF genes, as well as microsatellite instability of the donor tumor.Conclusion. The developed model of human colorectal cancer was characterized in terms of growth dynamics, cryopreservation tolerance, and histologic and molecular genetic parameters.
Possibilities of predicting the HER2 / neu status in a primary tumor in breast cancer patients using <sup>99m</sup>Tc-DARPinG3
Authors: Bragina O. D. , Tashireva L. A. , Chernov V. I. , Deyev S. M. , Tolmachev V. M. 
Published: January 2023

Aim. To determine informative prognostic criteria for assessing the HER2 / neu status in primary breast cancer using 99mTc-DARPinG3.Materials and methods. The study included 10 patients with breast cancer (T1-4N0-2M0) before systemic therapy, who underwent a radionuclide study using 99mTc-DARPinG3 at a dose of 3,000 μg. Five patients were characterized by HER2 / neu overexpression in primary breast cancer, whereas 5 patients were HER2-negative. For all patients, morphological and immunohistochemical studies and fluorescence in situ hybridization (FISH) of the primary tumor nodule were carried out. Single-photon emission computed tomography (SPECT) of the chest was performed for all patients 4 hours after the injection of 99mTc-DARPinG3.Results. The total activity of 99mTc-DARPinG3 was 522.4 ± 341.8 MBq. The comparative analysis showed that higher uptake of the labeled protein in HER2-positive breast cancer was significant (p = 0.0159, Mann – Whitney U test). The analysis of the ratios showed significant differences in the tumor-to-background ratios in patients with HER2-positive breast cancer (p < 0.0159, Mann – Whitney U test). Based on the logistic regression analysis, a mathematical model was developed to predict the status of HER2 / neu in primary breast cancer patients (specificity and sensitivity 100%; p = 0.0004) using 99mTc-DARPinG3 at a dose of 3,000 mcg 4 hours after the injection of the radiopharmaceutical.Conclusion. The results of the study allow to consider the tumor-to-background ratio 4 hours after the injection of 99mTc-DARPinG3 as an additional prognostic parameter for determining the HER2 / neu status in primary breast cancer.
Local biocompatibility and biochemical profile of hepatic cytolysis in subcutaneous implantation of polylactide matrices
Authors: Ivanova E. A. , Dzyuman A. N. , Dvornichenko M. V. 
Published: January 2023

The aim of the study was to investigate local biocompatibility and systemic effects of nonwoven polylactide (PLA) matrices on blood and liver parameters after their subcutaneous implantation in Wistar rats.Materials and methods. Bioabsorbable fibrous PLA matrices were produced by electrospinning and had dimensions (10 × 10 mm², thickness of no more than 0.5 mm; fiber diameter in the matrix ~1 μm) appropriate for subcutaneous implantation in white laboratory rats. Polymer implants were sterilized in ethylene oxide vapor. Thirty days after the implantation of PLA matrices, local biocompatibility according to GOST ISO 10993-6-2011, cellular parameters (total leukocyte count, hemogram, erythrocyte count, hemoglobin concentration), and biochemical blood parameters (lactate concentration, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels) were studied, and a standard histologic evaluation of the liver was performed.Results. PLA matrix samples were mild local irritants on a scale of 1–1.9 points according to GOST ISO 10993-6-2011 criteria 30 days after the subcutaneous implantation. The median density of distribution of multinucleated giant cells (MNGCs) in the connective tissue around and in PLA matrices was 1,500 (1,350; 1,550) per 1 mm² of a slice. Pronounced leukocytic reaction due to lymphocytosis was noted (an increase by 1.7 times compared with a sham-operated (SO) control group, р < 0.02). The absence of a significant neutrophil count in the blood revealed sterile inflammation proceeding in the subcutaneous tissue around the PLA materials. Normalization of hepatic cytolysis markers (ALT and AST activity) in the blood without pronounced changes in the structure of the liver and the number of binuclear hepatocytes was noted. These markers were increased in SO controls (ALT up to 123% and AST up to 142%, p < 0.001 compared with values in the intact group).Conclusion. Nonwoven PLA matrices are biocompatible with subcutaneous tissue, undergo bioresorption by MNGCs, and have a distant protective effect on the functional state of the liver in laboratory animals. Hypotheses on the detected systemic effect during subcutaneous implantation of PLA matrices were discussed; however, specific mechanisms require further study.
Effect of preterm birth in rats on proliferation and hyperplasia of cardiomyocytes
Authors: Ivanova V. V. , Milto I. V. , Serebryakova O. N. , Sukhodolo I. V. 
Published: January 2023

Aim. To identify the effect of preterm birth on proliferation and hyperplasia of cardiomyocytes in the early postnatal period of ontogenesis in rats.Materials and methods. Preterm birth (on day 21 and 21.5 of gestation) in Wistar rats was induced by subcutaneous administration of mifepristone. Immunohistochemistry was used to identify and calculate the number of Ki67-positive and Mklp2-positive cardiomyocytes in the left ventricle of preterm and full-term rats on days 1, 2, 3, 4, 5, and 6 of postnatal ontogenesis. Statistical analysis of morphometric parameters was performed using the Shapiro – Wilk test and Mann – Whitney test with the Bonferroni correction.Results. We revealed an increase in the number of Ki67-positive cardiomyocytes in the left ventricle of the rats: on day 1 of postnatal ontogenesis (in the rats born on day 21 of gestation) and on days 3–5 of postnatal ontogenesis (in the rats born on day 21.5 of gestation). Preterm birth in rats did not result in a change in the number of Mklp2-positive cardiomyocytes in the left ventricular wall.Conclusion. A change in the pattern of Ki67 expression by cardiomyocytes in the rats born 12 or 24 hours before full term was demonstrated in the early postnatal period of ontogenesis. An isolated increase in Ki67 expression without a change in Mklp2 expression by cardiomyocytes in the left ventricular wall of preterm rats indicates acceleration of cardiomyocyte hypertrophy. Shorter duration of prenatal development is associated with more pronounced morphological and functional rearrangements in the rat myocardium.