bluuebunny/crossref_metadata_2025_split huggingface dataset:
OECD-407 Driven 28-day-repeated-dose non-clinical safety evaluation of Tinospora cordifolia (Giloy) stem aqueous extract in Sprague-Dawley rats under GLP compliance
Authors: Balkrishna Acharya , Haldar Swati , Varshney Anurag 
Published: May 2023

Introduction:Tinospora cordifolia (Wild.) Hook.f. &amp; Thomson (Giloy), has been widely used in the Ayurvedic system of medicine. However, some sporadic under-powered case studies have recently reported Tinospora cordifolia associated toxicity. Thus, following OECD 407 guidelines, a 28-day-repeated-dose-14-day-recovery toxicological evaluation of the aqueous extract of T. cordifolia stem (TCWE) was conducted under good laboratory practice (GLP), in Sprague-Dawley (SD) rats.Methods: 100, 300, and 1000 mg/kg/day of TCWE was given orally to designated treatment groups of either sex. Two separate 14-day recovery satellite groups received either vehicle control or 1000 mg/kg/day of TCWE.Results: In this study, TCWE was found safe up to a dose of 1000 mg/kg/day with no mortality or related toxicological manifestation in terms of clinical signs, ocular effects, hematology, urinalysis, clinical chemistry parameters, or macro- or microscopic changes in any organs. The satellite group did not show any adverse effect after 14-day recovery period. Thus, the No-Observed-Adverse-Effect-Level (NOAEL) of TCWE was determined to be 1000 mg/kg/day.Discussion: In conclusion, this study established the non-clinical safety of the aqueous extract of T. cordifolia stem, which confirms the age-old safe medicinal use of this herb, and also paves the path for future clinical research on formulations containing Tinospora cordifolia.
Optimization of embryonic thermal programming confirms increased liver fattening in mule ducks and changes in lipid metabolism
Authors: Andrieux C. , Marchand M. , Larroquet L. , Veron V. , Biasutti S. , Morisson M. , Coustham V. , Panserat S. , Houssier M. 
Published: May 2023

Introduction: The embryonic thermal programming (TM) in birds has been shown to impact several physiological parameters such as resistance to thermal stress, muscle growth or immunity. In mule ducks, it has recently been shown that TM can induce metabolic programming resulting in increased liver weight and fat storage after overfeeding. However, a decrease in hatchability and foie gras quality was also observed, suggesting that this technique needs to be optimized. Here, we tested a new thermal manipulation condition determined with the objective of avoiding negative impacts while maintaining or improving liver properties.Methods: The eggs of the control group were incubated at 37.6°C during the whole incubation period while those of the experimental group (TM group) were incubated at 39.3°C 16 h/24 h from the 11th day of incubation to the 21st. After hatching, all the animals were fed and raised under the same conditions until the age of 12 weeks. At this stage, one part of the animals was overfed and then slaughtered 2 h (to measure rapid changes in metabolism) or 10 h after the last meal (to obtain the best technological yields), while the other part was ration-fed and slaughtered 2 h after the last meal, at the same age.Results: An 8% increase in foie gras production was measured in the TM group compared to the control group without altering the quality of the final product (nor hatchability), confirming the successful optimization of the metabolic programming. Interestingly, these results allowed us not to reject the previously suggested hypothesis of a potential delay in metabolic processes involved in liver fattening in programmed animals, in particular by measuring a trend reversal regarding the amount of total hepatic lipids in both groups at 2 h and then 10 h after the last meal.Discussion: This study therefore validates the optimization of metabolic programming by embryonic thermal manipulation for duck liver fattening. The understanding of the mechanisms of embryonic thermal programming in birds remains today very incomplete and the search for epigenetic marks (main hypothesis of the concept of programming) at the origin of the observed phenotypes could be the next step of this work.
Future horizons in the analysis of technical-tactical performance in women’s football: a mixed methods approach to the analysis of in-depth interviews with professional coaches and players
Authors: Iván-Baragaño Iyán , Ardá Antonio , Anguera M. Teresa , Losada José Luis , Maneiro Rubén 
Published: May 2023

IntroductionScientific knowledge about the criteria that determine success in women’s football is beginning to develop.MethodsThis study was carried out with the aim of detecting regularities in the offensive success in elite women’s football, as well as carrying out an interrelational analysis of linked behaviors, based on in-depth interviews with professional coaches and players. Eight in-depth interviews were conducted with professional Spanish coaches and players. The interviews were analyzed by indirect observation from a process of “quantitizing,” through the construction of an indirect observation ad hoc instrument. The segmentation of the transcription of the interviews was carried out in textual units, and the creation of a matrix of codes. Two types of analysis were performed: first, a lag sequential analysis (LSA) was performed and, then, a polar coordinates analysis (PCA), which allowed to find, respectively, a wide number of established communicative patterns with offensive performance in women’s football, as well as an interrelational map between the established codes.ResultsThe results obtained allowed us to suggest a statistically significant association between success in women’s football and criteria such as the physical characteristics of a particular player, the individual action space, the duration of the attack, the type of dynamic start, individual and collective technical and tactical aspects, decision making and the type of attack used.DiscussionBased on these results, the influence of these criteria on performance in women’s soccer can be studied in future studies. In addition, with the aim of increasing the validity of these conclusions, new studies on this subject may be carried out following strategies such as the Delphi Method.
Beyond the global attachment model: domain- and relationship-specific attachment models at work and their functions
Authors: Greškovičová Katarína , Lisá Elena 
Published: May 2023

IntroductionSince prior research has shown the importance of specific attachment models, we wanted to explore specific adult attachments (colleagues, leader, and workplace) in the working setting. The study aimed to investigate the position of specific adult attachments in the attachment hierarchy and their associations with various organizational variables. Finally, we assumed that dimensions of the colleagues-domain attachment model would cluster into attachment types at work, according to secure, preoccupied, and avoidant attachment orientations.MethodsWe carried out cross-sectional time-lagged research design. The sample consisted of 1,352 participants based on convenience and voluntary sampling procedures. Participants aged 18–78 worked in various work teams and positions. The battery consisted of the Adult Attachment in the Workplace Questionnaire, the Workplace Attachment Styles Questionnaire, the Scale of Belonging to the Organization, the Leader as Security Provider Scale, the shortened Experiences in Close Relationships-Revised Questionnaire, the Czech Leadership Questionnaire, the Citizenship Organizational Behavior Questionnaire, the General Work Performance questionnaire, the Utrecht Work Engagement Scale. Data were analyzed in JASP 0.16.3 and IBM SPSS Statistics 22. Among other statistical methods, we performed factor analysis and two-step cluster analysis. The alpha level for statistical testing was set to 0.05.ResultsThe results show that the work-specific attachment models differ from the romantic domain attachment model. Moreover, the work-specific attachment models also differ among themselves. Depending on the attachment to colleagues, it is possible to distinguish two attachment orientations (secure and insecure) among working adults. These two types differ in all the variables studied (relationships with colleagues, romantic partners, belonging to people and place, and performance).ConclusionThe study advances our knowledge of attachment working models and their application in the organizational context. We confirmed hierarchical attachment mental presentations and show the distinction in attachment working models at work. Colleagues and leaders form two separate domains within the workplace. Attachment to a leader is associated with the leadership style and secure workplace attachment. Attachment to colleagues might be more important in insecure workplace attachment and insecure belonging. Fostering secure attachment at work might bring together more positive outcomes for the company regarding performance and relationships at work.
Longitudinal assessment and determinants of short-term and longer-term psychological distress in a sample of healthcare workers during the COVID-19 pandemic in Quebec, Canada
Authors: Rapisarda Filippo , Bergeron Nicolas , Dufour Marie-Michèle , Guay Stéphane , Geoffrion Steve 
Published: May 2023

IntroductionPrevious research has demonstrated the negative impact of the COVID-19 pandemic emergency on the wellbeing of healthcare workers. However, few research contributions reported a longitudinal evaluation of psychological distress and examined determinants of its duration and course over time. The present study aims to explore the impact of the pandemic emergency on HCWs mental health by adopting a longitudinal design and assessing mental health as combination of overlapping clinical symptoms (post-traumatic stress disorder, depression and anxiety).MethodsData were collected weekly through a mobile application during and after the first wave of COVID-19 in the province of Quebec, Canada, in 2020. Analysis was conducted on a final sample of 382 participants. Participants were grouped into “resilient” (RES) if they did not manifest clinical-level psychological distress during monitoring, “short-term distress” (STD) if distress exceeded the clinical threshold for 1–3 weeks, and longer-term distress (LTD) if it occurred for four or more weeks, even if not consecutively. Descriptive statistics for all variables were computed for each subgroup (RES, STD and LTD), and pairwise comparisons between each group for every descriptive variable were made using chi square statistics for categorical variables and t-test for continuous variables. Predictors of distress groups (STD and LTD vs RES) were assessed running multinomial hierarchical logistic regression models.ResultsIn our sample, almost two third (59.4%) HCWs did not manifest moderate or severe distress during the monitoring time. Short-term distress, mostly post-traumatic symptoms that lasted for less than 4 weeks, were the most common distress response, affecting almost one third of participants. Longer psychological distress occurred only in a smaller percentage (12.6%) of cases, as a combination of severe posttraumatic, depressive and anxiety symptoms. Perceived occupational stress was the most significant risk factor; moreover individual, peritraumatic work and family risk and protective factors, were likely to significantly affect the stress response.DiscussionResults tend to provide a more complex and resiliency-oriented representation of psychological distress compared to previous cross-sectional studies, but are in line with stress response studies. Findings allow us to better describe the profiles of distress response in STD and LTD groups. Participants that manifest short term distress experience acute stress reaction in which the interplay between personal, family and professional life events is associated with the stress response. Conversely, longer term distress response in HCWs presents a more complex mental health condition with an higher level of impairment and support needs compared to participants with short-term distress.
Anxiety among hospitalized COVID-19 patients: a case–control study from a tertiary teaching hospital in Malaysia
Authors: Tan Hui Jan , Shahren Abdool Alleem Hj , Khoo Ching Soong , Ng Chen Fei , Zaidi Wan Asyraf Wan , Kori Najma , Periyasamy Petrick , Eu Choon Leng , Payus Alvin Oliver , Hod Rozita 
Published: May 2023

IntroductionAnxiety has been increasingly recognized as part of the psychosocial health issues in COVID-19 patients. However, the impact of this topic may be underestimated in low- and middle-income countries. This study aimed to estimate the prevalence of and risk factors of anxiety in COVID-19 patients compared to controls in a local tertiary teaching hospital in Malaysia.MethodsIn this case–control study, we analyzed data on adult patients aged 18 years and above hospitalized for COVID-19 infection with matched hospitalized controls. The demographic, clinical data and anxiety measures using the Generalized Anxiety Disorder-7 questionnaire were analyzed using univariate and multivariate analysis.Results86.6% in the COVID-19 group had anxiety, significantly higher than 13.4% in the control group (p = 0.001). The COVID-19 group was significantly associated with the GAD-7 severity (p = 0.001). The number of COVID-19 patients in the mild, moderate, and severe anxiety groups was 48 (84.2%), 37 (86%), and 18 (94.7%), respectively. Multiple logistic regression showed significant predictors for anxiety, including COVID-19 diagnosis and neurological symptoms. Anxiety was found 36.92 times higher in the patients with COVID-19 compared to those without COVID-19 (OR 36.92;95% CI 17.09, 79.78, p = 0.001). Patients with neurological symptoms were at risk of having anxiety (OR 2.94; 95% CI 1.03, 8.41, p = 0.044).DiscussionCOVID-19 patients experience a significant disruption in psychosocial functioning due to hospitalization. The burden of anxiety is notably high, compounded by a diagnosis of COVID-19 itself and neurological symptomatology. Early psychiatric referrals are warranted for patients at risk of developing anxiety symptoms.
A voxel-based morphometry investigation of brain structure variations in late-life depression with insomnia
Authors: Shao Heng , Li Na , Chen Meiling , Zhang Jie , Chen Hui , Zhao Minjun , Yang Jingjing , Xia Jian 
Published: May 2023

BackgroundLate-life depression (LLD) is linked to various medical conditions and influenced by aging-related processes. Sleep disturbances and insomnia symptoms may be early indicators or risk factors for depression. Neuroimaging studies have attempted to understand the neural mechanisms underlying LLD, focusing on different brain networks. This study aims to further delineate discriminative brain structural profiles for LLD with insomnia using MRI.MethodsWe analyzed 24 cases in the LLD with insomnia group, 26 cases in the LLD group, and 26 in the healthy control (HC) group. Patients were evaluated using the Hamilton Depression Rating Scale (HAMD-17), Hamilton Anxiety Rating Scale (HAMA), Mini-Mental State Examination (MMSE), and Pittsburgh Sleep Quality Index (PSQI). Structural MRI data were gathered and analyzed using voxel-based morphometry (VBM) to identify differences in gray matter volume (GMV) among the groups. Correlation analyses were conducted to explore the relationships between GMV and clinical characteristics.ResultsSignificant difference in sex distribution was observed across the groups (p = 0.029). However, no significant differences were detected in age and MMSE scores among the groups. LLD with insomnia group exhibited significantly higher HAMA (p = 0.041) and PSQI scores (p &lt; 0.05) compared to the LLD group. ANOVA identified significant difference in GMV of anterior lobe of cerebellum (peak MNI coordinate: x = 52, y = −40, z = −30) among HC, LLD, and LLD with insomnia. Post-hoc two-sample t-tests revealed that the significant difference in GMV was only found between the LLD group and the HC group (p &lt; 0.05). The mean GMV in the cerebellum was positively correlated with HAMA scale in LLD patients (r = 0.47, p &lt; 0.05).ConclusionThere is significant difference in GMV in the LLD group, the association between late-life depression and insomnia may be linked to anxiety. This study provides insights into the discriminative brain structural profiles of LLD and LLD with insomnia, advancing the understanding of the underlying neural mechanisms and potential targets for intervention.
Reproductive decision-making following the diagnosis of an inherited metabolic disorder via newborn screening in Japan: a qualitative study
Authors: Hiromoto Kana , Nishigaki Masakazu , Kosugi Shinji , Yamada Takahiro 
Published: May 2023

IntroductionThe aim of the study was to describe the factors influencing the reproductive decision-making of carrier parents after the diagnosis of an inherited metabolic disorder in newborn screening in Japan.MethodsWe conducted a semi-structured interview with 12 parents and analyzed data based on content analysis methodology.ResultsWe identified 11 factors, including personal evaluation of recurrence risk, understanding of hereditary phenomena, concerns and desires for future planned children, concerns for older siblings, perceptions of diseases, degree of acceptance and denial of diseases, the opinions of others on having another child, optimism/faith in positive outcomes, self-evaluation of parental capability, factors unrelated to the disease, and the “right” time to expand the family.DiscussionPerceptions and acceptance of disease are both important factors in reproductive decision-making, though these factors fluctuate continuously during the childbearing period. Therefore, effective reproductive genetic counseling will be considerate of the parents' fluctuating perceptions on reproduction. To ensure that the decision-making process is for the benefit of the parents and future children, long-term involvement of health care professionals is needed to assess the client's acceptance of the disease and their understanding of genetic phenomena and recurrence rates.
Different positions in society, differing views of the world: the privatization of water and sanitation services in Minas Gerais, Brazil
Authors: Neves-Silva Priscila , Braga Juliana Gontijo , Heller Léo 
Published: May 2023

IntroductionIn 2020, the Brazilian government sanctioned Law No. 14.026, which established new guidelines for water and sanitation in the country and encouraged service privatization. Based on this, the State of Minas Gerais government, Brazil has proposed a bill for these services. Regarding this bill, different social actors in the State of Minas Gerais have expressed different assessments concerning the outcomes of the proposed management model and its impact on the realization of the human rights to safe water and sanitation.MethodsWe used content analysis techniques to assess the position of different actors—government, civil society, and private sector—in three public events that were recorded and posted on the YouTube platform.ResultsThe analyses of this study indicated that the positions of the current government are aligned with those of the private sector. Also, the analyses agree that privatization can lead to the universalization of access to services. These different actors did not mention the human rights to safe water and sanitation in their discourses. Civil society organizations are concerned about the guarantee of those rights. For them, privatization of water and sanitation services can lead to discontinuation of the access of these services to vulnerable populations and poor municipalities due to tariff increases.DiscussionThe discourses concerning water and sanitation privatization as an instrument for universalization are disputed. Private sector actors and government representatives, aligned with the neoliberal agenda, support privatization. Civil society organizations defend the improvement of public management as the path to universalization. Representatives of municipalities were not included in the discussion for the new bill. The different societal positions relate to the different views these actors have concerning privatization and the world.
Identification and Profiling of a Novel Bombyx mori latent virus Variant Acutely Infecting Helicoverpa armigera and Trichoplusia ni
Authors: Verdonckt Thomas-Wolf , Bilsen Anton , Van Nieuwerburgh Filip , De Troij Loes , Santos Dulce , Vanden Broeck Jozef 
Published: May 2023

Insect cell expression systems are increasingly being used in the medical industry to develop vaccines against diseases such as COVID-19. However, viral infections are common in these systems, making it necessary to thoroughly characterize the viruses present. One such virus is Bombyx mori latent virus (BmLV), which is known to be specific to Bombyx mori and to have low pathogenicity. However, there has been little research on the tropism and virulence of BmLV. In this study, we examined the genomic diversity of BmLV and identified a variant that persistently infects Trichoplusia ni-derived High Five cells. We also assessed the pathogenicity of this variant and its effects on host responses using both in vivo and in vitro systems. Our results showed that this BmLV variant causes acute infections with strong cytopathic effects in both systems. Furthermore, we characterized the RNAi-based immune response in the T. ni cell line and in Helicoverpa armigera animals by assessing the regulation of RNAi-related genes and profiling the generated viral small RNAs. Overall, our findings shed light on the prevalence and infectious properties of BmLV. We also discuss the potential impact of virus genomic diversity on experimental outcomes, which can help interpret past and future research results.
Features of dental care provision for the child population in subjects of the Russian Federation in 2020 and 2021
Authors: Yanushevich O. O. , Kiselnikova L. P. , Zueva T. E. , Zolotnitsky I. V. , Ogareva A. A. 
Published: May 2023

Relevance. Dental care is one of the most common and socially significant types of medical care to the population of the Russian Federation. The types of outpatient pediatric dental care are pre-medical primary dental care for children provided by dental therapists; specialized primary pediatric dental care provided by dentists and pediatric dentists. Dental care availability is a dental care effectiveness criterium involving the population presentation, including the children's population, to the medical institutions providing dental assistance.The study aimed to conduct an analysis of the changes in the volume and types of pediatric dental care in 84 regions of the Russian Federation in 2020 and 2021.Material and methods. Within the framework of the activities of the National Medical Research Center of the Moscow State Medical University, named after A.I. Evdokimov of the Ministry of Health of the Russian Federation on the profile "Dentistry", the study analyzed the reporting documentation of 84 regions of the Russian Federation on the main sections of the dental service for 2020 and 2021. The article presents an analysis of the types of dental care provided to children, the number of child population visits for dental care in the regions of the Russian Federation in 2020 and 2021 and the pattern of changes in the studied indicators.Results. In 2021, the number of regions where a child had less than one annual visit to a dental care provider increased by 27.66%. In 2021de, compared with 2020, there was a decrease in the number of child visits to dental care facilities by 15.78%. In general, in 84 regions of the Russian Federation in 2021, there is a decrease in the volume of pre-medical primary dental care provided by dental therapists and specialized primary dental care provided by pediatric dentists and oral surgeons and an increase in the specialized primary dental care volume to the child population by dentists (general practice dentists).