bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of optimal primary tumor resection candidates for metastatic gastric cancer: Nomograms based on propensity score matching
Authors: Li Zhehong , Zheng Honghong , Zhao Ziming , Chen Guanyang , Wang Zheng , Amin Buhe , Zhang Nengwei 
Published: April 2023

AbstractBackgroundThis study sought to develop and validate nomograms for screening patients with metastatic gastric cancer (mGC) who are candidates for primary tumor resection (PTR) and evaluating the prognosis of mGC patients after PTR.MethodsFrom 2010 to 2016, we screened mGC patients with complete data from the Surveillance, Epidemiology, and End Results (SEER) database. Depending on whether or not PTR was performed, we categorized patients into surgery and non‐surgery groups. A 1:1 propensity score matching (PSM) analysis was used to balance the characteristics of the two groups. The endpoints were overall survival (OS) and cancer‐specific survival (CSS). Two predictive nomograms were developed using logistic regression to assess the likelihood of benefit. Two additional prognostic nomograms were developed to assess prognosis in mGC patients after PTR by Cox regression. Finally, nomograms were evaluated using a variety of methodologies.ResultsOur study included 3594 mGC patients who met the criteria. PTR was associated with improved OS and CSS time (median OS time after PSM: 15 vs. 7 months, P < 0.05; median CSS time after PSM: 17 vs. 7 months, P < 0.05). The OS‐related predictive nomogram, including age, histologic type, grade, T stage, and chemotherapy, was developed. Moreover, the CSS‐related predictive nomogram, including age, histologic type, grade, and chemotherapy, was developed. Sex, histologic type, grade, T stage, N stage, and chemotherapy were found to be correlated with OS. Furthermore, the CSS correlated with histologic type, grade, T stage, N stage, and chemotherapy. Both predictive and prognostic nomograms were found to be valuable and reliable after different types of validation.ConclusionPredictive nomograms were developed and validated for identifying the optimal PTR mGC candidates. Prognostic nomograms were developed and validated for assessing the prognosis of mGC patients after PTR.
Three rare presentations of high‐altitude pulmonary edema at a high‐altitude clinic in the Everest region (4371 m): A case series
Authors: Subedi Sachin , Regmi Priyanka , Bhandari Sanjeeb S. , Dawadi Suvash 
Published: April 2023

Key Clinical MessageDiagnosis of HAPE can be challenging when the presentation deviates from usual natural history. Point of care ultrasonography serves as a great diagnostic tool in such settings. An umbrella treatment could be beneficial during such scenarios.
Adult nondysraphic cervicomedullary intramedullary lipoma: A case report and literature review
Authors: Roohollahi Faramarz , Sharifi Vahid , abousaeidi Setare R. , Jouibari Morteza F. 
Published: April 2023

Key clinical messageIntra‐dural perimedullary lipomas involving craniovertebral region are rare. There is no clear tumor‐cord border and patients are at high risk of neurological deficits after surgery. Partial resection of the mass and neural decompression are the main surgical strategies in symptomatic patients.
Pseudohyperkalemia associated with essential thrombocytosis; a hint for better clinical practice
Authors: Yaghoubi Fatemeh , Dalil Davood 
Published: April 2023

AbstractA 75‐year‐old man was admitted with a diagnosis of diabetic ketoacidosis and hyperkalemia. During the treatment, he developed refractory hyperkalemia. Following our review, diagnosis of pseudohyperkalaemia secondary to thrombocytosis was made. We report this case to remind the importance of clinical suspicion of this phenomenon to prevent its serious consequences.
Coexistence of junctional epidermolysis bullosa, autosomal recessive deafness type 57, and Angelman syndrome: A case report
Authors: Amato Maria Eugenia , Ricart Silvia , Vicente Maria Asunción , Martorell Loreto , Armstrong Judith , Fernández Isern Guerau , Mascaro José Manuel , Balsells Sol , Alonso Itziar , Serrano Mercedes , Ortigoza‐Escobar Juan Darío 
Published: April 2023

Key Clinical MessageThe presence of more than one genetic/genomic disorder is not uncommon. It is therefore essential to continuously consider new signs and symptoms over time. Administration of gene therapy could be extremely difficult in particular situations.AbstractA 9‐month‐old boy presented to our department for evaluation of developmental delay. We found that he was affected by intermediate junctional epidermolysis bullosa (COL17A1, c.3766 + 1G > A, homozygous), Angelman syndrome (5,5 Mb deletion of 15q11.2‐q13.1), and autosomal recessive deafness type 57 (PDZD7, c.883C > T, homozygous).
Recent advances of transition‐metal metaphosphates for efficient electrocatalytic water splitting
Authors: Zhang Yuanyuan , Wu Jie , Guo Bingrong , Huo Haohao , Niu Siqi , Li Siwei , Xu Ping 
Published: April 2023

AbstractSustainable production of H2 through electrochemical water splitting is of great importance in the foreseeable future. Transition‐metal metaphosphates (TMMPs) have a three‐dimensional (3D) open‐framework structure and a high content of P (which exists as PO3−), and therefore have been recognized as highly efficient catalysts for oxygen evolution reaction (OER) and the bottleneck of electrochemical water splitting. Furthermore, TMMPs can also contribute to hydrogen evolution reaction (HER) in alkaline and neutral media by facilitating water dissociation, and thus, overall water splitting can be achieved using this kind of material. In this timely review, we summarize the recent advances in the synthesis of TMMPs and their applications in OER and HER. We present a brief introduction of the structure and synthetic strategies of TMMPs in the first two parts. Then, we review the latest progress made in research on TMMPs as OER, HER, and overall water‐splitting electrocatalysts. In this part, the intrinsic activity of TMMPs as well as the current strategy for improving the catalytic activity will be discussed systematically. Finally, we present the future opportunities and the remaining challenges for the application of TMMPs in the electrocatalysis field.
New Hypothesis Insight of Structural Properties on Four Tau Peptide Fragments in Alzheimer's Disease: Origin from the Histidine Behaviors
Authors: Sun Yue , Li Changgui , Wang Jinping , Shi Hu 
Published: April 2023

AbstractDuring protein folding and misfolding, structural properties and aggregation tendency can be significantly influenced by histidine behaviors (tautomeric behaviors and protonation behaviors). The original reasons were derived from the net charge changes and the various N/N−H orientation on imidazole rings. In the current study, total 18 independent REMD simulations were performed to investigate the histidine behaviors on four Tau peptide fragments (MBD, including R1, R2, R3, and R4 fragments). We found that, compared to R1, R2, R3 except (ϵδ), and R4 systems with flexible structural features, only R3(ϵδ) has dominating conformational structure (possibility of 81.3 %) with three β‐strand structures in parallel β‐sheet structures at I4‐K6 and I24‐H26, as well as antiparallel β‐sheet structure at G19‐L21. Importantly, the H25 and H26 residues (in R3(ϵδ) system) are directly involved in the sheet structure formations and strong H‐bonded interactions (possibility range of 31.3 %–44.7 %). Furthermore, the donors and acceptors analysis confirmed that only R3(ϵδ) shows faraway amino acids interaction features in both H25 and H26 residues, and such cooperation effects of two histidine residues contribute to current structural features. The current study will be helpful to further enrichment of the histidine behavior hypothesis, it provides new insight for understanding protein folding and misfolding.
Nitric Oxide‐Releasing Poly(L‐glutamic acid) Hybrid Hydrogels for Accelerating Diabetic Wound Healing†
Authors: Teng Lin , Song Yingying , Hu Lingli , Bai Qian , Zhang Xueliang , Dong Chang‐Ming 
Published: April 2023

Comprehensive SummaryDiabetic wound healing is threatening worldwide and challenging in wound dressings. Aiming to inhibit heavy inflammation and bacterial infection in diabetic wounds, herein, we facilely construct a kind of NO‐releasing poly(L‐glutamic acid) (PGA) based graphene oxide (GO) hybrid hydrogel of HDGS at a lower solid percentage, presenting large microporous size of about 20 μm, mild photothermal conversion property, and good biocompatibility. Besides improving hemostasis performance, the less amount of GO endowed the hydrogel with near infrared (NIR) responsivity to control fast and pulsatile NO release for killing bacteria and inhibiting heavy inflammation to proheal diabetic wound, in which a broad spectrum of antibacterial activities toward killing S. aureus, E. coli and MRSA was achieved via a combined effect of photothermia and NO release. In vivo effective hemostasis was attained in a rat liver bleeding model with short hemostatic time of ~20 s and lower blood loss of 1.5%—2.0%. Moreover, the treatment of HDGS plus 4 times of mild NIR irradiation (10 min, 808 nm, 1 W/cm2 per time) performed superior full diabetic wound healing within 11—14 d, in which the regenerated skins were characteristic of thick epidermis/dermis, dense blood vessels, some hair follicles‐embedding, and high level of collagens.
Leiomyosarcoma, a rare smooth muscle cancer in the pancreas: An adjuvant radiotherapy treatment approach
Authors: Taghizadeh Kermani Ali , Khooei Alireza , Aliakbarian Mohsen , Arastouei Soudeh 
Published: April 2023

AbstractBackgroundLeiomyosarcoma of visceral organs is uncommon, and pancreatic primary occurrence is even rarer. In terms of curative treatment, patients are generally managed with surgery alone, without significant data on the role or efficacy of adjuvant chemotherapy.Case presentationThis manuscript presents a case of a 22‐year‐old female with advanced primary leiomyosarcoma of the pancreas, treated with radical surgery and adjuvant radiation therapy.ConclusionWith a low‐survival rate, consideration of radiation therapy in some advanced and unresectable cases could be potentially beneficial.
Chromosomal instability and its effect on cell lines
Authors: He Zichen , Wilson Andrew , Rich Fenella , Kenwright Diane , Stevens Aaron , Low Yee Syuen , Thunders Michelle 
Published: April 2023

AbstractBackgroundCancer cell lines are invaluable model systems for biomedical research because they provide an almost unlimited supply of biological materials. However, there is considerable skepticism regarding the reproducibility of data derived from these in vitro models.Recent findingsChromosomal instability (CIN) is one of the primary issues associated with cell lines, which can cause genetic heterogeneity and unstable cell properties within a cell population. Many of these problems can be avoided with some precautions. Here we review the underlying causes of CIN, including merotelic attachment, telomere dysfunction, DNA damage response defects, mitotic checkpoint defects and cell cycle disturbances.ConclusionIn this review we summarize studies highlighting the consequences of CIN in various cell lines and provide suggestions on monitoring and controlling CIN during cell culture.
Rfoot‐seq: Transcriptomic RNase Footprinting for Mapping Stable RNA‐Protein Complexes and Rapid Ribosome Profiling
Authors: Li Qianru , Stroup Emily K. , Ji Zhe 
Published: April 2023

AbstractRibosome profiling isolates ribosome‐protected fragments for sequencing and is a valuable method for studying different aspects of RNA translation. However, conventional protocols require millions of input cells and time‐consuming steps to isolate translating ribosome complexes using ultracentrifugation or immunoprecipitation. These limitations have prevented their application to rare physiological samples. To address these technical barriers, we developed an RNase footprinting approach named Rfoot‐seq to map stable transcriptomic RNA‐protein complexes that allows rapid ribosome profiling using low‐input samples (Li, Yang, Stroup, Wang, & Ji, 2022). In this assay, we treat a cell lysate with concentrated RNase without complex crosslinking and retained only RNA footprints associated with stable complexes for sequencing. The footprints in coding regions represent ribosome‐protected fragments and can be used to study cytosolic and mitochondrial translation simultaneously. Rfoot‐seq achieves comparable results to conventional ribosome profiling to quantify ribosome occupancy and works robustly for various cultured cells and primary tissue samples. Moreover, Rfoot‐seq maps RNA fragments associated with stable non‐ribosomal RNA‐protein complexes in noncoding domains of small noncoding RNAs and some long noncoding RNAs. Taken together, Rfoot‐seq opens an avenue to quantify transcriptomic translation and characterize functional noncoding RNA domains using low‐input samples. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Harvesting and lysing adherent cellsAlternate Protocol 1: Harvesting and lysing suspension cellsAlternate Protocol 2: Harvesting and lysing primary tissue samplesBasic Protocol 2: RNase treatment and footprint purification for low‐input samplesAlternate Protocol 3: RNase treatment and footprint purification for ultra‐low‐input samplesBasic Protocol 3: Library preparation for high‐throughput sequencingSupport Protocol: Preparation of dsDNA markers for library size selectionBasic Protocol 4: Data analysis and quality control after sequencing
Permanent fillers—What is their role
Authors: Chan Lina , Li Andraia , Schlesinger Todd 
Published: April 2023

AbstractBackgroundWith the growing cosmetic industry, there have been numerous advancements in nonsurgical interventions including the use of permanent dermal filler injections. Permanent fillers are relatively novel in clinical use when compared to temporary and semi‐permanent fillers. The only FDA‐approved, non‐biodegradable permanent filler uses polymethylmethacrylate (PMMA) microspheres. PMMA is suspended in bovine collagen and lidocaine solution for injection, absorbed by the body, and replaced overtime by collagen. ArteFill® was the first FDA approved permanent filler in 2005 for correction of nasolabial folds, with studies demonstrating rejuvenation for up to five years, trumping its predecessors in longevity. A decade later with rebranding, Bellafill® was indicated for correction of nasolabial fold and atrophic acne scars.AimsTo review the development of permanent fillers, mechanism, and safety.Materials & MethodsA qualitative systematic review was conducted on PMMA microspheres indications, contraindications, and off‐label use on benefits, risks, and adverse events.ResultsThe durability of permanent fillers has a strong appeal for both patients and practitioners, however, usage for certain patient populations demonstrates increased risk for both early and late complications compared to temporary and semi‐permanent fillers. A skin test composed of purified 3.5% bovine collagen gel administered in the volar forearm is recommended one month prior to injection, to identify any patients who may have a positive response. Bovine collagen demonstrates low immunogenicity with the incidence of preexisting hypersensitivity reported to be between 2‐4%. Bovine collagen in dermatologic formulations use pepsin‐solubilized formulations in which telopeptides are cleaved to further help reduce aberrant immunogenic responses.DiscussionEarly and late complications may outweigh the benefits in use of permanent fillers, however these complications are patient dependent.ConclusionPermanent fillers may be useful in volume replacement for nasolabial folds and atrophic acne scars, however a provider must use good clinical judgement in deciding which patients are ideal candidates.
Exploring sensitivities to hydropeaking in Atlantic salmon parr using individual‐based modelling
Authors: Hedger Richard D. , Sundt‐Hansen Line E. , Juárez‐Gómez Ana , Alfredsen Knut , Foldvik Anders 
Published: April 2023

AbstractHydropeaking (the release of water pulses at hydropower plants) results in temporary reductions in river channel water‐covered area downstream, which may cause fish mortality through stranding. We used a mechanistic modelling approach to examine how both the form of the hydropeaking cycle and the characteristics of the affected fish control how hydropeaking may cause stranding mortality of fish. We modelled the response of Atlantic parr to hydropeaking in a regulated watercourse in central Norway (the River Nidelva) using an individual‐based population model designed explicitly to examine fish behaviour and stranding mortality during hydropeaking. A response to hydropeaking, involving migration from the riverbanks towards the mid‐channel on down‐ramping and a return to the riverbanks on up‐ramping, was based on individuals being parameterized to migrate to habitat properties that spatially changed throughout the hydropeaking cycle. We found that stranding mortality was strongly dependent on both the form of the hydropeaking cycle and on the fish response. Total stranding mortality was more dependent on the down‐ramping speed than the duration of the minimum flow period. Total stranding mortality was greatest when there was a low movement speed, leading to individuals being stranded, combined with a high probability of dying per unit of time when stranded. Given the sensitivity of mortality to the fish response and the lack of detailed field studies quantifying this, we conclude that this area requires further controlled studies for parameterizing models used to predict effects of hydropeaking on fish. Simulation‐based modelling, where there is a 100% control of and knowledge of fish characteristics, where a range of deterministic functions can be evaluated and in which boundary conditions can be easily controlled, may be a useful research tool to complement empirical studies.
Organocatalytic Upgrading of Biomass Derived Building Blocks
Authors: Meninno Sara 
Published: April 2023

AbstractThe depletion of finite primary fossil fuels we are facing makes necessary a deep metamorphosis in fundamental parts of the chemical industry. A progressive transition from petro‐based starting materials toward renewable biomass‐derived sources will have to take place in the synthesis of added‐value chemicals, important for our everyday life, such as pharmaceuticals, polymers, agrochemicals etc. Moreover, greener processes, carried out under friendlier reaction conditions, must be designed to address current concerns about the climate change and the resulting pressing need to reduce the environmental footprint of chemical processes. To this end, organocatalysis could offer a valuable opportunity for upgrading biomass‐derived platform molecules in line with the principles of Green Chemistry. This review presents some of recent and remarkable advancements in this emerging area. Organocatalysis has proven to be an efficient tool to transform low value bio‐based renewable platform building blocks into new high value bio‐based chemicals, with potential applications as synthetic intermediates, innovative materials and pharmaceutically active compounds.
Design of cell expansion processes for adherent‐growing cells with mDoE‐workflow
Authors: Kuchemüller Kim B. , Pörtner Ralf , Möller Johannes 
Published: April 2023

AbstractAdherent cells, mammalian or human, are ubiquitous for production of viral vaccines, in gene therapy and in immuno‐oncology. The development of a cell‐expansion process with adherent cells is challenging as scale‐up requires the expansion of the cell culture surface. Microcarrier (MC)‐based cultures are still predominate. However, the development of MC processes from scratch possesses particular challenges due to their complexity. A novel approach for the reduction of development times and costs of cell propagation processes is the combination of mathematical process models with statistical optimization methods, called model‐assisted Design of Experiments (mDoE). In this study, an mDoE workflow was evaluated successfully for the design of a MC‐based expansion process of adherent L929 cells at a very early stage of development with limited prior knowledge. At the start, the analytical methods and the screening of appropriate MCs were evaluated. Then, cause‐effect relationships (e.g., cell growth related to medium conditions) were worked out, and a mathematical process model was set‐up and adapted to experimental data for modeling purposes. The model was subsequently used in mDoE to identify optimized process conditions, which were proven experimentally. An eight‐fold increase in cell yield was achieved basically by reducing the initial MC concentration.
Correlation of DNA methylation and lymph node metastasis in papillary thyroid carcinoma
Authors: Zhao Feng , Zhu Siyi , Fang Jun , Dong Huilei , Zhu Chenfang 
Published: April 2023

AbstractBackgroundPapillary thyroid carcinoma (PTC) is the most common type of thyroid cancer with a primarily good prognosis, and its 10‐year survival rate is over 90%. However, PTC is prone to early lymph node metastasis.MethodsThyroid cancer tissues from PTC patients with lymphatic metastasis and normal tissues were collected for DNA methylation analysis. Different methylation sites, different methylation regions, gene‐enriched pathways, and protein–protein interactions (PPIs) were analyzed.ResultsThere were 1004 differentially methylated sites in the PTC group versus the control group; these involved 479 hypermethylated sites in 415 related genes, 525 hypomethylated sites in 482 related genes, 64 differentially methylated regions located in the CpG island region, 34 differentially methylated genes closely related to thyroid cancer, and 17 genes with differentially methylated genes in the DNA promoter region.ConclusionNDRG4 hypermethylation and FOXO3, ZEB2, and CDK6 hypomethylation were associated with PTC lymph node metastasis.
Ultrasound fusion imaging for improving diagnostic and therapeutic strategies of focal liver lesions: A preliminary study
Authors: Huang Danqing , Wen Baojie , Zhang Han , Liu Han , Wang Wenping , Shen Haiyun , Kong Wentao 
Published: April 2023

AbstractPurposeTo assess the effect of ultrasound (US) fusion imaging on the clinical diagnostic and therapeutic strategies of focal liver lesions, which are difficult to detect or diagnose by conventional US.MethodsFrom November 2019 to June 2022, 71 patients with invisible or undiagnosed focal liver lesions who underwent fusion imaging combining US with CT or MR were included in this retrospective study. The reasons for US fusion imaging were as follows: (1) lesions that were undetectable or inconspicuous on B‐mode US; (2) post‐ablation lesions that could not be assessed accurately by B‐mode US; (3) to evaluate whether the lesions detected by B‐mode US that were consistent with those presented on MRI/CT images.ResultsOf the 71 cases, 43 cases were single lesions, and 28 cases were multiple lesions. Among the 46 cases which were invisible on conventional US, the display rate of lesions using US‐CT/MRI fusion imaging was 30.8%, and that combined with CEUS was 76.9%. US‐guided biopsy was performed in 30 patients after the detection and localization determined by fusion imaging, with a positive rate of 73.3%. Six patients with recurrence after ablation therapy were all detected and located accurately after fusion imaging, and 4 of them successfully underwent ablation therapy again.ConclusionFusion imaging contributes to the understanding of the anatomical relationship between lesion location and blood vessels. Additionally, fusion imaging can improve the diagnostic confidence, be helpful to guide interventional operations, and hence be conducive to clinical therapeutic strategies.