bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measured GFR in murine animal models: review on methods, techniques, and procedures
Authors: Teixido-Trujillo Silvia , Luis-Lima Sergio , López-Martínez Marina , Navarro-Díaz Maruja , Díaz-Martín Laura , Escasany-Martínez Elia , Gaspari Flavio , Rodríguez-Rodríguez Ana Elena 
Published: August 2023

AbstractChronic kidney disease (CKD) is one of the most common chronic diseases worldwide, with increasing rates of morbidity and mortality. Thus, early detection is essential to prevent severe adverse events and the progression of kidney disease to an end stage. Glomerular filtration rate (GFR) is the most appropriate index to evaluate renal function in both clinical practice and basic medical research. Several animal models have been developed to understand renal disease induction and progression. Specifically, murine models are useful to study the pathogenesis of renal damage, so a reliable determination of GFR is essential to evaluate the progression of CKD. However, as in clinical practise, the estimation of GFR in murine by levels of serum/urine creatinine or cystatin-C could not be accurate and needed other more reliable methods. As an alternative, the measurement of GFR by the clearance of exogenous markers like inulin, sinistrin, 51Cr-EDTA, 99mTc-DTPA, 125I-iothalamate, or iohexol could be performed. Nevertheless, both approaches—estimation or measurement of GFR—have their limitations and a standard method for the GFR determination has not been defined. Altogether, in this review, we aim to give an overview of the current methods for GFR assessment in murine models, describing each methodology and focusing on their advantages and limitations.
The genomic landscape of mammal domestication might be orchestrated by selected transcription factors regulating brain and craniofacial development
Authors: Benítez-Burraco Antonio , Uriagereka Juan , Nataf Serge 
Published: August 2023

AbstractDomestication transforms once wild animals into tamed animals that can be then exploited by humans. The process entails modifications in the body, cognition, and behavior that are essentially driven by differences in gene expression patterns. Although genetic and epigenetic mechanisms were shown to underlie such differences, less is known about the role exerted by trans-regulatory molecules, notably transcription factors (TFs) in domestication. In this paper, we conducted extensive in silico analyses aimed to clarify the TF landscape of mammal domestication. We first searched the literature, so as to establish a large list of genes selected with domestication in mammals. From this list, we selected genes experimentally demonstrated to exhibit TF functions. We also considered TFs displaying a statistically significant number of targets among the entire list of (domestication) selected genes. This workflow allowed us to identify 5 candidate TFs (SOX2, KLF4, MITF, NR3C1, NR3C2) that were further assessed in terms of biochemical and functional properties. We found that such TFs-of-interest related to mammal domestication are all significantly involved in the development of the brain and the craniofacial region, as well as the immune response and lipid metabolism. A ranking strategy, essentially based on a survey of protein-protein interactions datasets, allowed us to identify SOX2 as the main candidate TF involved in domestication-associated evolutionary changes. These findings should help to clarify the molecular mechanics of domestication and are of interest for future studies aimed to understand the behavioral and cognitive changes associated to domestication.
The incidence of type 1 diabetes in children under 15 years of age is rising again—a nationwide study
Authors: Roche Edna F. , McKenna Amanda M. , O’Regan Myra , Ryder Kerry J. , Fitzgerald Helen M. , Hoey Hilary M. C. V. 
Published: August 2023

AbstractInternational incidence rates (IRs) and trends of childhood type 1 diabetes (T1D) vary. Recent data from Ireland and other high incidence countries suggested a stabilisation in IRs of T1D in children aged under 15 years. Our primary objective was to report the IR of T1D in children in Ireland from 2019 to 2021 and evaluate if age, sex and season of diagnosis had changed. Incident cases of T1D in those aged under 15 years were identified prospectively by clinicians nationally and reported to the Irish Childhood Diabetes National Register (ICDNR). Following case verification, capture-recapture methodology was applied, and IRs calculated. Numbers of children including age, sex and season of diagnosis per year were evaluated. There were 1027 cases, 542 males (53%). The direct standardised incidence rates (SIRs) increased by 21% overall and were 31.1, 32.2 and 37.6/100,000/year, respectively, with no significant sex difference. The highest IRs were in the 10–14-year category until 2021, then changed to the 5–9-year category (40% of cases). Whilst autumn and winter remain dominant diagnostic seasons, seasonality differed in 2021 with a greater number presenting in spring.Conclusion: The incidence of childhood T1D in Ireland is increasing, observed prior to the COVID-19 pandemic, and shifting to an earlier age at diagnosis for the first time. The pattern of seasonality also appears to have changed. This may reflect an increased severity of diabetes with important implications for healthcare providers.

What is Known:• Ireland has a very high incidence of T1D in childhood, which had stabilised following a rapid rise, similar to other high incidence countries.• The incidence rate is consistently highest in older children (10–14 years).

What is New:• Irish IR is no longer stable and has increased again, with the highest incidence occurring in the younger 5-9 age category for the first time.• The seasonality of diagnosis has changed during the COVID-19 pandemic years of 2020–2021.
A user experience analysis for a mobile Mixed Reality application for cultural heritage
Authors: De Luca Valerio , Barba Maria Cristina , D’Errico Giovanni , Nuzzo Benito Luigi , De Paolis Lucio Tommaso 
Published: August 2023

AbstractMixed Reality has emerged as a valuable tool for the promotion of cultural heritage. In this context, in particular, the metaphor of virtual portals allows the virtual visit of monuments that are inaccessible or no longer exist in their original form, integrating them into the real environment. This paper presents the development of a Mixed Reality mobile application that proposes a virtual reconstruction of the church of Sant’Elia in Ruggiano, in the southern province of Lecce (Italy). By placing the virtual portal in the same place where the entrance of the church was located, the user can cross this threshold to enter inside and make a virtual journey into the past. The user experience was evaluated by administering a questionnaire to 60 users who tried the application. From the data collected, four user experience factors were identified (interest, focus of attention, presence and usability), which were compared between young and old, male and female users, and between users who had already visited the church in person and all other users. In general, the scores reveal a total independence of the other three factors from usability and a very high level of interest.
The impact of an ultrasound atlas for scoring salivary glands in primary Sjögren’s syndrome: a pilot study
Authors: Schmidt Nanna S. , Fana Viktoria , Danielsen Mads Ammitzbøll , Lindegaard Hanne M. , Voss Anne , Horn Hans Christian , Knudsen John B. , Byg Keld-Erik , Morillon Melanie Birger , Just Søren Andreas , Døhn Uffe M. , Terslev Lene 
Published: August 2023

AbstractThe objective of this pilot study was to assess the impact of a salivary gland ultrasound (SGUS) atlas for scoring parenchymal changes in Sjögren’s syndrome by assessing the reliability of the scoring system (0–3), without and with the use of the SGUS atlas. Ten participants with varying experience in SGUS contributed to the reliability exercise. Thirty SGUS images of the submandibular and parotid gland with abnormalities ranging from 0 to 3 were scored using the written definitions of the OMERACT SGUS scoring system and using the SGUS atlas based on the OMERACT scoring system. For intra-reader reliability, two rounds were performed without and with the atlas—in the 2nd round the 30 images were rearranged in random order by a physician not included in the scoring. Inter-reader reliability was also determined in both rounds. Without using the atlas, the SGUS OMERACT scoring system showed fair inter-reader reliability in round 1 (mean kappa 0.36; range 0.06–0.69) and moderate intra-reader reliability (mean kappa 0.55; range 0.28–0.81). With the atlas, inter-reader reliability improved in round 1 to moderate (mean kappa 0.52; range 0.31–0.77) and intra-reader reliability to good (mean kappa 0.69; range 0.46–0.86). Higher intra-reader reliability was noted in participants with previous SGUS experience. The SGUS atlas increased both intra- and inter-reader reliability for scoring gland pathology in participants with varying SGUS experience suggesting a possible future role in clinical practice and trials.

Key Points• Ultrasonography can detect parenchymal changes in salivary glands in patients with Sjögren’s disease.• An ultrasound atlas may improve reliability of scoring parenchymal changes in salivary glands.
Transcriptome and methylome sequencing reveals altered long non-coding RNA genes expression and their aberrant DNA methylation in equine sarcoids
Authors: Semik-Gurgul Ewelina , Gurgul Artur , Szmatoła Tomasz 
Published: August 2023

AbstractRecent publications confirmed that long non-coding RNAs (lncRNAs) perform an essential function in gene-specific transcription regulation. Nevertheless, despite its important role, lncRNA has not yet been described in equine sarcoids, the skin neoplasia of horses. Therefore, the aim of this study is to deepen the knowledge about lncRNA expression in the pathogenesis of equine sarcoids and provide new insight into the regulatory function of lncRNA in the bovine papillomavirus–dependent neoplasia of horse dermal tissues. RNA sequencing (RNA-seq) data from 12 equine sarcoid samples and the corresponding controls were reanalyzed in this study. A total of 3396 differentially expressed (DE) lncRNAs and 128 DElncRNA-DE genes (DEGs) pairs were identified. Differentially expressed lncRNAs predicted target genes were enriched in pathways associated with inter alia the extracellular matrix disassembly and cancer pathways. Furthermore, methylation data from the same samples were integrated into the analysis, and 12 DElncRNAs were described as potentially disturbed by aberrant methylation. In conclusion, this study presents novel data about lncRNA’s role in the pathogenesis of equine sarcoids.
Contested agri-food futures: Introduction to the Special Issue
Authors: Gugganig Mascha , Burch Karly Ann , Guthman Julie , Bronson Kelly 
Published: August 2023

AbstractOver recent decades, influential agri-food tech actors, institutions, policymakers and others have fostered dominant techno-optimistic, future visions of food and agriculture that are having profound material impacts in present agri-food worlds. Analyzing such realities has become paramount for scholars working across the fields of science and technology studies (STS) and critical agri-food studies, many of whom contribute to STSFAN—the Science and Technology Studies Food and Agriculture Network. This article introduces a Special Issue featuring the scholarship of STSFAN members, which cover a range of case studies and interdisciplinary and transdisciplinary engagements involving such contested agri-food futures. Their contributions are unique in that they emerged from the network’s specific modus operandi: a workshopping practice that supports the constructive, interdisciplinary dialogue necessary for critical research and rigorous analyses of science and technology in agri-food settings. This introduction offers an overview of STS and critical agri-food studies scholarship, including their historical entanglements in respective studies of food scandals, scientific regimes and technological determinism. We illustrate how interdisciplinary engagement across these fields has contributed to the emergent field of what we term agri-food technoscience scholarship, which the contributions of this Special Issue speak to. After a brief discussion of STS concepts, theories and methods shaping agri-food policy, technology design and manufacturing, we present the eleven Special Issue contributions in three thematic clusters: influential actors and their agri-food imaginaries; obfuscated (material) realities in agri-food technologies; and conflictual and constructive engagements in academia and agri-food. The introduction ends with a short reflection on future research trajectories in agri-food technoscience scholarship.
Single-trial ERP Quantification Using Neural Networks
Authors: Depuydt Emma , Criel Yana , De Letter Miet , van Mierlo Pieter 
Published: August 2023

AbstractTraditional approaches to quantify components in event-related potentials (ERPs) are based on averaging EEG responses. However, this method ignores the trial-to-trial variability in the component’s latency, resulting in a smeared version of the component and underestimates of its amplitude. Different techniques to quantify ERP components in single trials have therefore been described in literature. In this study, two approaches based on neural networks are proposed and their performance was compared with other techniques using simulated data and two experimental datasets. On the simulated dataset, the neural networks outperformed other techniques for most signal-to-noise ratios and resulted in better estimates of the topography and shape of the ERP component. In the first experimental dataset, the highest correlation values between the estimated latencies of the P300 component and the reaction times were obtained using the neural networks. In the second dataset, the single-trial latency estimation techniques showed an amplitude reduction of the N400 effect with age and ascertained this effect could not be attributed to differences in latency variability. These results illustrate the applicability and the added value of neural networks for the quantification of ERP components in individual trials. A limitation, however, is that simulated data is needed to train the neural networks, which can be difficult when the ERP components to be found are not known a priori. Nevertheless, the neural networks-based approaches offer more information on the variability of the timing of the component and result in better estimates of the shape and topography of ERP components.
Treatment Outcomes of Children with Primary Versus Secondary Callous-Unemotional Traits
Authors: Fleming Georgette E. , Neo Bryan , Kaouar Silvana , Kimonis Eva R. 
Published: August 2023

AbstractObjective: Recent efforts to improve outcomes for young children with conduct problems and callous-unemotional (CU) traits involve adapting treatments to meet the unique needs of this subgroup. However, these efforts have ignored accumulating evidence for distinct primary and secondary variants within the CU subgroup. Existing treatment adaptations uniformly target risk factors associated with primary CU traits and no studies have investigated variant-specific patterns of responsiveness to treatment adaptations among young children with CU-type conduct problems. Method: Participants were 45 families with a 3- to 7-year-old clinic-referred child (M = 4.84 years, SD = 1.08, 84% boys) with conduct problems and CU traits. Primary and secondary CU variants were defined based on baseline parent-rated anxiety scores. All families received Parent-Child Interaction Therapy adapted for CU traits (PCIT-CU) at an urban university-based research clinic. Families completed five assessments measuring child conduct problems and affective outcomes. Results: Linear mixed-effects modeling showed that the rate and shape of change over time in conduct problems differed between variants, such that children with secondary CU traits showed deterioration in defiant and dysregulated behaviors from post-treatment to follow-up, whereas primary CU traits were associated with maintained gains. There were no variant differences in rate of improvement in CU traits. Affective empathy did not improve for either variant. Internalizing problems meaningfully improved by follow-up for children with secondary CU traits. Conclusions: Findings suggest that PCIT-CU is a promising intervention for children with conduct problems and primary CU traits, but may require further personalization for children with secondary CU traits. This trial was registered with the Australian New Zealand Clinical Trials Registry (ACTRN12616000280404).
Nach den Berufswahlpraktika ist vor der Wahl des Ausbildungsberufs: Vorhersage des Entscheids für die Weiterverfolgung eines Praktikumberufs im Berufswahlprozess
Authors: Hofmann Jan , Neuenschwander Markus P. 
Published: August 2023

ZusammenfassungIn diesem Artikel wurde untersucht, welche Faktoren dazu führen, dass Jugendliche einen in ihren Schnupperlehren und Berufswahlpraktika ausprobierten Beruf (Praktikumberuf) in ihrem Berufswahlprozess als potenzieller Ausbildungsberuf weiterverfolgen. Als theoretische Grundlage für die Analysen diente Gottfredsons Theory of Circumscription, Compromise, and Self-Creation (TCCSC). Nach Geschlechter getrennt wurde eine Längsschnittstichprobe von 128 weiblichen und 202 männlichen Jugendlichen analysiert, die im siebten und neunten Schuljahr standardisierte Fragebögen ausfüllten und angaben, nach der obligatorischen Schulzeit eine berufliche Grundbildung zu beginnen. Die gerechneten Regressionsmodelle zeigten, dass das Lehrstellenangebot den Weiterverfolgungsentscheid für weibliche wie auch für männliche Jugendliche am stärksten beeinflusst (positiver Effekt). Ausschließlich bei den weiblichen Jugendlichen hat sich gezeigt, dass neben dem Lehrstellenangebot das Ausmaß führend-verkaufender Anforderungen des ausprobierten Praktikumberufs den Entscheid signifikant negativ beeinflusst. Die Ergebnisse stützen die in der TCCSC postulierte Zugänglichkeitsthese, wonach Personen Berufe mit größerer Wahrscheinlichkeit weiterverfolgen, wenn für diese Berufe eine gute Zugänglichkeit im Sinne eines großen Lehrstellenangebots besteht. Eine mögliche Erklärung für den negativen Einfluss führend-verkaufender Anforderungen auf den Weiterverfolgungsentscheid bei weiblichen Jugendlichen ist vor dem Hintergrund des in der TCCSC enthaltenen Kompatibilitätsgedankens, dass weibliche Jugendliche ihre führend-verkaufenden Fähigkeiten möglicherweise als zu gering einschätzen. Infolgedessen nehmen sie Berufe mit hohen führend-verkaufenden Anforderungen als für sich inkompatibel wahr und wenden sich von diesen Berufen ab. Diese Studie konnte mit der Untersuchung der Rolle von Praktikaberufen für den weiteren beruflichen Verlauf eine Lücke in der Berufswahlforschung angehen und bietet wichtige Anknüpfungspunkte für zukünftige Analysen.
Triple assessment breast clinics: The value of clinical core biopsies
Authors: Maha Rahmani , Alison Johnston , Michael Sugrue , Manvydas Varzgalis 
Published: August 2023

Abstract
Background
Triple Assessment Breast Clinics are designed for rapid diagnosis of symptomatic patients. When there is no concordance between clinical and radiological assessment, clinicians perform clinical core biopsies. In patients with a clinically suspicious examination (S4, S5) and normal imaging, clinically guided core biopsy should be performed as per NCCP guidelines. However, substantial research does not exist on the diagnostic value or use of clinical core biopsies in non-suspicious palpable (S3) lesions and practices differ in each health system.

Aims
The aim of this research was to assess the diagnostic value of clinical core biopsies in nonsuspicious, probably benign palpable breast lesions (S3) where image guided cores were not indicated (R1/R2).

Methods
The cohort consisted of patients undergoing clinical core biopsies at a Symptomatic Breast Unit from January 2014 to 2019. Data regarding patient demographics, outcome of triple-assessment and incidence of malignancy were obtained from a prospectively maintained database and results were analysed using Minitab 2018.

Results
Three hundred and sixty patients had a clinical core biopsy performed in this period. Clinical examination scores for these patients were S1/S2 (66), S3 (277), S4 (15), and S5 (2). Radiology Scores were R1/R2 (355) and R3(5). Two patients with clinical score S3 (0.6%) were diagnosed with breast cancer due to their clinical cores. Both patients had normal mass imaging. There was no association between uncertain palpable breast lesions (S3), and atypia or malignancy on biopsy results when breast imaging was normal (P = 0.43, χ2 test).

Conclusion
Despite clinical core biopsies being used in triple assessment, there is no certainty in their value except that there is high clinical suspicion. Imaging modalities are constantly improving and are already well established. When the patient is assigned a clinical score of S3 and has normal radiology, a clinical core biopsy is not required in most cases.
Long noncoding RNA CASC11 suppresses sorafenib-triggered ferroptosis via stabilizing SLC7A11 mRNA in hepatocellular carcinoma cells
Authors: Chen Fei , Wang Liang 
Published: August 2023

AbstractAs a frontline treatment for patients with advanced hepatocellular carcinoma (HCC), sorafenib is an effective drug approved by the Food and Drug Administration (FDA). Ferroptosis, a newly defined programmed cell death process with the hallmark of the accumulation of iron-dependent lipid peroxides, can be induced by sorafenib treatment. Our previous study identified oncogenic roles of long noncoding RNA (lncRNA) Cancer susceptibility candidate 11 (CASC11) in HCC progression. However, the relationship between CASC11 and sorafenib-induced ferroptosis in HCC remains unclear. In the present study, we aim to investigate the role of CASC11 in sorafenib-induced ferroptosis in HCC cell lines and determine the involved molecular mechanisms. Here, we demonstrated that sorafenib decreased CASCL11 expression. Knockdown of CASC11 enhanced sorafenib-induced ferroptosis, while overexpression of CASC11 exerted the opposite effect in HCC cells. Moreover, CASC11 led to the accumulation of intracellular malondialdehyde (MDA), lipid reactive oxygen species (ROS) and Fe2+ while depleting glutathione (GSH), thereby suppressing sorafenib-induced ferroptosis and cell death. Ferrostatin-1 (Ferr-1), a ferroptosis inhibitor, reversed the enhanced anticancer effect of sorafenib caused by the silence of CASC11 in HCC cells. Mechanistically, CASC11 upregulated the expression of solute carrier family 7 member 11 (SLC7A11) which is critical for ferroptosis inhibition. CASC11 associated with and stabilized SLC7A11 mRNA. In summary, our data revealed, for the first time, that CASC11 inhibits the sorafenib-induced ferroptosis in HCC cells via regulating SLC7A11, providing a new basis for clinical therapeutic strategies for patients with HCC.