bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of thyroid hormones on the functional state of bovine granulosa cells in vitro
Authors: Mityashova O. , Montvila E. , Lebedeva I. 
Published: January 2023

Any dysfunction of the thyroid gland causes abnormal changes in the functioning of the reproductive system, primarily the ovaries. Therefore, the question of the possible direct effect of thyroid hormones on the bovine ovarian function by modulating the functional state or functional activity of granulosa cells seems relevantPurpose: to study in vitro the effect of thyroxine and triiodothyronine on the proliferative and steroidogenic activity, as well as apoptotic changes of bovine granulosa cells.Materials and methods. Granulosa cells were isolated from follicles with a diameter of 1-5 mm and precultured for two days in a medium containing 10 % serum. The cells were then placed in serum-free medium containing thyroxine (25-200 ng/mL) or triiodothyronine (0.5-4.0 ng/mL) and incubated for another 48 h. After culturing, the content of estradiol-17β and progesterone in the media was determined by ELISA. The proliferative activity and apoptotic changes in the cells were assessed by immunocytochemical assay, based on the expression level of proliferating cell nuclear antigen PCNA and pro-apoptotic protein Bax, respectively.Results. It was found that the proportion of cells with a positive reaction to PCNA increased 1.1 times (P<0.01) compared with that in the control at a triiodothyronine concentration of 1 ng/ml and did not change with its further increase to 4 ng/ml. In addition, the introduction of triiodothyronine at a concentration of 1 ng/ml into the medium led to a decrease in the relative number of Bax-positive cells from 25.6 ± 0.3% to 23.3 ± 0.6 % (P<0.01). A further increase in this concentration to 4 ng/ml enhanced the observed anti-apoptotic effect 1.1 times (P<0.05). The pattern of the effect of thyroxine on the proliferative activity and apoptotic changes of granulosa cells in culture was similar to that for triiodothyronine. Concurrently, the growth-stimulating and anti-apoptotic effects of thyroxine were achieved at a concentration of 50-200 ng/ml. At the same time, both thyroid hormones did not affect the secretion of estradiol-17β or progesterone by the cells.Conclusions. Thus, thyroxine and triiodothyronine can stimulate in vitro the proliferation of bovine granulosa cells, as well as inhibit the expression of the proapoptotic Bax protein in these cells, which is not associated with the regulation of the production of ovarian steroid hormones. Overall, these data suggest that thyroid hormones at physiological concentrations are able to exert a regulatory effect on the growth and atresia of bovine small antral follicles and, therefore, directly modulate the ovarian activity.
Effect of post-activation culture conditions on the development of parthenogenetic embryos in cattle
Authors: Lopukhov A. 
Published: January 2023

Purpose: to conduct a comparative analysis of the effect of commercial media BO-IVC and СR1aa at the stage of the activation and subsequent culture of artificially activated oocytes on the formation and quality of parthenogenetic bovine embryos.Materials and methods. 3 groups of disemeters of 50 goals in each were formed. In the first experimental group, the disemeted was in a meticulous manner with a ram-industrialist (artificial kriproporchid), in the second experimental-with a penEexctomed ram-industrialist. In the third (control) group, a producer ram was used. In the first experimental group of a ram-industrialist (artificial kriproporchid) with attached taps were released into a group of sheep twice a day for 1.5-2 hours. In the second experimental group of a penEctomed ram, it was placed in the corral to the disemetery in the morning for 3 hours. In the third group, the lamb producer was constantly with the disemets for two weeks, then he was changed on a new ram i.e. Used the methodology used in the farm. During the experiment, they observed the behavior of animals of all groups. In the experimental groups, after the detection of disemeters in the hunt, their natural insemination of the manufacturer was carried out. Based on the results of the subsequent oster, the effectiveness of the reproduction of sheep was evaluated.Results. The cleavage rate did not differ between the experimental groups, varying from 73,0 to 76,5%. Also, there was not found a significant effect of the conditions for post-activation culture of oocytes on their development before late morula and late blastocyst stage, which was for the CR1aa/CR1aa, CR1aa/BO-IVC and BOIVC/ BO-IVC groups 28,9±1,7, 40,4±7,5 and 36,0±6.4%, respectively. Meanwhile, we found out the effect of tested culture conditions on the ability of parthenogenetic embryos to overcome the 8-16 cell block and their quality on the late stages of embryo development. The rate of embryos with less than 16 nuclei was the highest in the CR1aa/CR1aa group (56,8±2,1 %). The replacement of CR1aa medium to BO-IVC medium (BO-IVC/BO-IVC group) significantly reduced this level (p<0,05). The positive effect was enhanced when CR1aa medium was used at the stage of culture in the presence of 6-DMAP and cycloheximide, and subsequent embryo development was in BO-IVC medium (CR1aa/BO-IVC group) (p<0.001). Furthermore, when we used the mixed variant of culture, the total cell number in parthenogenetic morula and blastocyst stage embryos increased (p<0.05).Conclusion. Thus, the BO-IVC medium at the stages of post-activation and subsequent development of artificially activated bovine oocytes is comparable to the CR1aa medium in terms of the efficiency of obtaining parthenogenetic embryos at the blastocyst stage. Nevertheless, its replacement at the post-activation stage with CR1aa medium makes it possible to improve the quality of parthenogenetic embryos.
On the Question of the Features of Coping Mechanisms of Modern Adolescents
Authors: Pirkina Vera Grigoryevna 
Published: January 2023

The article is devoted to the study of the characteristics of coping mechanisms in adolescence. To overcome stress, difficult situations, coping mechanisms are formed, i. e. actions and behavioral models for resolving difficult situations. The purpose of the study is to study the characteristics of coping mechanisms in adolescents. An empirical study was conducted in 2021 on the basis of educational organizations of the Republic of Mari El and the Kirov region. The sample consisted of 667 students in grades 7–9 (322 boys and 345 girls) aged 13–15. The research method is a mass screening examination. To collect data, the E. Heim test (E. Heim), adapted in the laboratory of clinical psychology of the Psychoneurological Institute named after. V. M. Bekhterev, under the guidance of d.m.s. professor L. I. Wasserman. The results obtained showed that in order to overcome stressful situations, adolescents most often use adaptive emotional and relatively adaptive behavioral coping mechanisms, less often than others use relatively adaptive emotional coping mechanisms. Adolescent girls significantly more often than boys use maladaptive emotional and behavioral coping mechanisms. The results of the study can be useful in the development of recommendations aimed at the formation of constructive coping behavior of adolescents.
WEB-BASED SYSTEM of DECISION SUPPORT FOR CALCULATING COMBAT AND NON-COMBAT LOSSES DURING MILITARY CAMPAIGNS IN THE MIDDLE AGES
Authors: MELNYKOV OLEKSANDR , KAPELESHCHUK ANDREI 
Published: January 2023

The project is devoted to calculating the number of losses of medieval armies using artificial intelligence methods. Several possible calculation methods have been considered, and historical sources have been analyzed. The analysis of factors that affected combat and non-combat losses and the analysis of scientific and popular science literature have been carried out, and the methods proposed by specialists have been translated into the language of formulas. The process of building neural networks, selecting their architecture, searching and synthesizing data for training samples have been considered, and the process of training and verifying the obtained data have been considered. Two models of neural networks and an information model in the form of UML diagrams of the future web application have been developed. Diagrams of use cases, classes, and components for each element of the web system have been described. The models have been implemented using the modern Django framework. A full-fledged web application with microblogging has been developed and tested. The conclusions have been drawn about the efficiency and scalability of the developed system, and the functionality of the system has been demonstrated using a real historical example.
The project has developed the models of an information system for analyzing the number of troops and calculating military losses of the remote past. Mathematical models have been described, historical sources have been analyzed, and detailed models of the application have been created using the UML modeling language, which allows you to understand its interface in detail at the modeling stage.
Based on the data obtained in the analysis of historical literature, the neural network architectures have been developed to determine non-combat losses in the medieval army and determine combat losses based on the data on the number of each branch’s soldiers of the armed forces who were involved during the battle.
Web applications for calculating combat and non-combat losses have been developed, interface design and mini-block for publishing system News have been developed either. The non-combat losses suffered by the Mongol army in the first month of the Western campaign (1236) have been calculated as the example.
Long-term impact of COVID-19 on cardiovascular health of healthcare workers of a tertiary care hospital of Bangladesh
Authors: Uddin Mir Jamal , Islam A K M Monwarul , Ananya Kaniz Fatema , Rahman Shovan , Ghafoor Nusrat , Shahriar Saqif , Majumder Mizanur Rahman , Hasan Mahmudul , Mahmud Khan AKS Zahid 
Published: January 2023

Background: The coronavirus disease (COVID-19) is a multisystem disease that sometimes affects the cardiovascular system (CVS) during acute illness and follows a protracted course in a proportion of patients causing ‘post-COVID syndrome’. Exercise tolerance test (ETT) can assess functional capacity and imaging modalities like echocardiography and cardiac magnetic resonance (CMR) can assess structural and functional status of cardiovascular system (CVS) efficiently. However, the pattern of cardiovascular involvement and its effect on functional capacity in COVID-19 survivors long after the index illness are inadequately known. This study was carried out to detect the functional status and imaging findings in COVID-19 recovered healthcare workers.
Methods: This cohort study was carried out in the Department of Cardiology of the National Institute of Cardiovascular Diseases (NICVD), Dhaka, Bangladesh from July 2021 to December 2021. Thirty male healthcare workers, previously not known to have any cardiac disease, who suffered from reverse transcriptase polymerase chain reaction (RT-PCR)-confirmed COVID-19 of mild severity at least 3 months back were included. Besides clinical evaluation, ETT, echocardiography and CMR were done.
Results: The mean age was 37.2±4.7 years. The mean time interval from RT-PCR positivity was 317.4±85.7 days. The functional capacity was good in the study subjects; the achieved metabolic equivalents (METs) were 13.0±1.5. The mean left ventricular ejection fraction (LVEF) was 66.9% ± 6.2%; all were within the normal range. The mean LV global longitudinal strain (LVGLS) was -19.2% ± -1.9%; 6 patients had LVGLS <-18%. One patient had mildly reduced tricuspid annular plane systolic excursion (TAPSE) of 16 mm but 3 patients had reduced lateral tricuspid annulus peak systolic velocity (RVS’). CMR revealed subtle abnormalities in 14 (46.7%) patients. Eight patients (26.67%) had increased T2 signal indicating myocardial oedema, 2 (6.7%) had subepicardial late gadolinium enhancement (LGE).
Conclusion: In healthcare workers who recovered from mild COVID-19, despite preserved functional capacity, subclinical cardiac abnormalities detected by echocardiography and CMR may be present. Long-term follow-up is warranted to define the clinical significance of these findings.
BIRDEM Med J 2023; 13(1): 3-11
Disparity between clinical and final diagnosis of patients undergoing hysterectomy
Authors: Shermin Shahana , Farjana Shayla , Noor Aysha , SenGupta Ruma 
Published: January 2023

Background: Hysterectomy is by far the commonest major gynaecological procedure done for several indications. A thorough history, careful physical examination and some simple investigations are all that is needed to establish the diagnosis justifying the need for hysterectomy in most of the cases. But even with the advent of modern technologies, a good proportion of patients are misdiagnosed preoperatively which is evident after histopathological confirmation. This study aimed to evaluate the clinical diagnoses which ultimately lead to hysterectomy and to find out to what extent they are consistent with the final diagnoses after confirmation by histopathological examination.
Methods: This observational study was carried out in the Department of Obstetrics and Gynaecology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh from January to June 2018. Total 100 patients, irrespective of age and parity, were included whose total abdominal hysterectomy was planned. History, clinical findings, investigation reports and histopathological reports were recorded. Then clinical and histopathological diagnoses were tabulated and expressed as proportion.
Results: The incidence of total abdominal hysterectomy was highest (49%) among the age group of 41-50 years. Average age of the study subjects was 45.3 years. Majority of the hysterectomies were done for fibroid uterus (48%) and abnormal uterine bleeding due to ovulatory dysfunction (AUB-O) (16%). Histopathology confirmed fibroid or leiomyoma in 39% cases versus clinical diagnosis of 48% and 18% of the specimens revealed no organic lesion on histopathology.
Conclusion: Though not remarkable, still a certain proportion of clinical diagnoses differ with histopathological findings in patients undergoing hysterectomy even in tertiary level.
BIRDEM Med J 2023; 13(1): 27-33
Post-COVID fatigue: a new era of complication by altered thyroid function
Authors: Alam Mohammad Masum , Rahman Mohammad Matiur , Mollah Forhadul Hoque , Chowdhury Indira 
Published: January 2023

Background: Since late 2019, the development of severe acute respiratory distress syndrome (SARS) occurred, which began as a small cluster of cases in Wuhan, China and subsequently spread across the globe in the shape of a global pandemic. Coronavirus disease 2019 (COVID-19) initiates an immune response over-activity leading to release of pro-inflammatory cytokines, particularly interleukin-6 (IL-6), which leads to overt thyroid dysfunction by disruption of thyroid transport proteins. Level of triiodothyronin (T3) is inversely proportional to IL-6 with a modest decrease of thyroid stimulating hormone (TSH) and thyrotropin (T4). Fatigue is usually expressed by lack of one or more of habits or routines, particularly a lack of exercise. It is also frequently associated with depression. Fatigue might be an indication of various underlying illnesses that necessitate medical attention. COVID-19-related thyroid disorders could biochemically manifest as thyrotoxicosis, hypothyroidism, as well as non-thyroidal illness syndrome. Post-COVID fatigue has become an alarming complication which remains undiagnosed causing more complication at later stage. There are very limited knowledge regarding altered thyroid function in patients who have recovered from COVID-19. Aims of this study were to know about the derangement of thyroid hormones.
Methods: This cross-sectional study was conducted in the Department of Biochemistry and Molecular Biology, BSMMU, Dhaka from July, 2020 to June, 2021. A total of 100 post-COVID patients (age 18 – 70 years) with fatigue were enrolled for this study. Among them, 50 were treated at home and 50 were treated in hospital. For this study, serum TSH and FT4 were measured by the automated analyser. The data were expressed as frequency and percentage, mean for normally distributed data. p value d”0.05 was considered statistically significant.
Results: The value of TSH and FT4 in home and hospital treated patients were 1.59, 2.96 mIU/L and 9.27, 17.28 pmol/L respectively. There was significant difference of level of serum TSH (p < 0.0001) and FT4 (p < 0.0001) between home and hospital treated post COVID-19 patients with fatigue.
Conclusion: Home treated post-COVID patients with fatigue expressed higher level of serum TSH and FT4 than that of hospital treated patients.
BIRDEM Med J 2023; 13(1): 34-37
Benefícios da massagem infantil em recém-nascidos e lactentes: Revisão integrativa
Authors: Carvalho Verônica da Silva , Silva Maria Paula Custódio , Alves Luana Tamires da Silva , Rocha Nylze Helena Guillarducci , Contim Divanice , Oliveira Nuno Miguel Lopes de 
Published: January 2023

O objetivo deste estudo foi identificar na literatura os benefícios da aplicação da massagem infantil em recém-nascidos pré-termo, recém-nascidos à termo e lactentes. Realizou-se uma revisão integrativa da literatura a partir de uma busca nos bancos de dados PubMed, BVS Saúde, Web of Science e Scopus, utilizando como estratégias as palavras chaves indexadas no DeCS: recém-nascido, massagem e fisioterapia e no MeSH: “infant”, “newborn”, “massage”, “Physical Therapy Specialty”. Foram incluídos artigos originais, publicados nos idiomas inglês, português ou espanhol, entre os anos de 2014 e 2019. A análise dos dados foi elaborada de forma descritiva, para isso, utilizou-se um quadro, contendo: citação, ano de publicação, país de origem, participantes do estudo, método e principais resultados. O nível de evidência foi identificado com base no delineamento do estudo. Em relação ao delineamento dos estudos, quatro foram revisões sistemáticas, quatro ensaios clínicos randomizados, dez ensaios controlados não randomizados (quase experimento) e cinco estudos qualitativos ou descritivos. Dentro do nível de evidência oito estudos foram classificados como fortes, dez como moderados, e cinco como fracos. Dessa forma, observa-se a necessidade da elaboração de protocolos para realizar as intervenções de massagem infantil em recém-nascidos pré-termo, a termo e lactentes. Sugere-se a realização de ensaios clínicos randomizados sobre a aplicação da técnica nessas populações, com amostras e tempo de estudo maiores, para alcance de melhores evidências.
Aprendizagem de máquina na determinação de parâmetros de resistência de filitos brandos
Authors: Pinto Lívia Aparecida Gonçalves , Silva José Margarida da 
Published: January 2023

Aspectos geológico-geotécnicos devem ser levados em consideração desde os estudos de exploração geológica, em empreendimentos mineiros. Alguns acidentes ocorridos em minas, nas últimas décadas, poderiam ser evitados, caso as condições geológico-geotécnicas do maciço fossem compreendidas. Ainda existe grande dificuldade em se classificar alguns tipos de rochas, sobretudo as rochas consideradas brandas, por meio dos sistemas de classificação geomecânica conhecidos. A grande maioria das classificações existentes foram desenvolvidos baseadas em características de rochas duras. Para maciços rochosos pouco resistentes, é necessário adaptar os sistemas atuais ou desenvolver novos sistemas de classificação, que sejam específicos para maciços rochosos brandos praticamente contínuos. O objetivo deste artigo é propor a utilização de técnicas de Aprendizagem de Máquina para previsão de parâmetros geotécnicos de rochas brandas, especialmente filito. Foram utilizados dados históricos de resultados de ensaios geotécnicos de laboratório de rochas de minas do Quadrilátero Ferrífero, que, por meio da interação otimizada, e com auxílio de técnica de Inteligência Artificial, como a Rede Neural Artificial e Regressão Linear, sejam capazes de gerar resultados de interesse para análises de estabilidade e modelagens geotécnicas. Das técnicas utilizadas, resultados mostraram que o método de Regressão Linear se mostrou satisfatório na determinação de parâmetros de resistência de filitos brandos e com boas perspectivas de ampliação e utilização para outros parâmetros, assim como outros tipos de rochas.
Socialtjänsten som en hållbar kunskapsorganisation
Authors: Avby Gunilla , Melke Anna 
Published: January 2023

Social Services as a sustainable knowledge organization: from vision to reality?
The imminent Social Services Act is expected to stipulate that decision-making should be "in accordance with scientific evidence and proven experience". If the proposal is fulfilled, the Act will contain requirements similar to existing acts in other welfare sectors, such as requirements for increased systematic follow-ups, developmental work and the use of knowledge from various knowledge sources. These are characteristics that symbolize a practice that both uses verified knowledge and develops new knowledge by testing, documenting and evaluating new ideas and working methods in a systematic process in everyday life. In this article, we argue that the goal presupposes an aspiration towards a knowledge organization; therefore we want to contribute with an analysis of what this means for social services.
The “knowledge-seeking social worker” is a key actor in the transition towards a knowledge organization, because it is in the everyday social work practice, that is, the interactions between the social worker and the client, that the knowledge organization largely becomes visible. However, a large-scale transition towards a sustainable knowledge organization cannot depend on only professionals and their ability to engage clients in work processes, it will require changes at a system level, where actors in politics, academia and practice take responsibility for future investments. This article highlights four areas of importance: 1) co-ordination of research and practice, 2) continuing education to build expert knowledge and broaden career paths, 3) development of political ownership for quality and direction, and 4) organization and management for knowledge use and knowledge development.