bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementasi Quality Control (QC) Penggunaaan Alat Point Of Care Testing (POCT) sebagai Upaya Jaminan Mutu Hasil Pemeriksaan di Puskesmas Cempaka Kota Banjarmasin
Authors: Salman Yuliana , Farihi Muhammad Ilham , Yahya Yudi 
Published: August 2023

Clinical Laboratory is a health laboratory that carries out clinical specimen examination to obtain information about individual health, especially to support diagnose disease, and restore health, therefore quality control (QC) must be applied, especially in utilization of POCT tools. This research approach uses a qualitative approach with data collection methods through interviews, observation and documentation. The object of this research was the Cempaka Public Health Center based on recommendations and permits from the Banjarmasin City Health Office with the research respondents being the Head of the Clinical Laboratory, the Laboratory Quality Assurance Team and the Laboratory Assistant. The variable of this research was the implementation of QC using the POCT tools tool at the pre-analytical, analytical and post-analytic stages. Based on the results of interviews, observations and documentation, data was obtained that the Head of the Puskesmas formed an Internal Audit Team to carry out monitoring and evaluation every 6 months, but the last activity was carried out in 2019. Aspects audited in Monitoring Evaluation Indicators of the clinical is about SOP (Standard Operational Procedure) for the implementation of examinations in the laboratory using the FMEA (Failure, Mode, Effect Analysis) method. The implementation of Quality Control in the pre-analytical, analytical and post-analytic stages is in accordance with the applicable policies and SOPs. Monitoring and Evaluation of Clinical Quality Indicators should be carried out regularly and continuously so that the results of the examination obtained are guaranteed quality and can be used for determining the right diagnosis.
ANALISA KELAYAKAN INVESTASI SISTEM INFORMASI MANAJEMEN RUMAH SAKIT (SIMRS) MENGGUNAKAN METODE INFORMATION ECONOMICS (IE)
Authors: Pratama Yudha Herlambang Cahya , Supriyanto Heri , Supriyanto Heri 
Published: August 2023

Perkembangan teknologi dan bisnis yang begitu cepat, menuntut perusahan untuk terus berkembang seperti pada fasilitas Kesehatan yaitu rumah sakit. Salah satu upaya yang dilakukan guna menjawab tuntutan pelayanan kesehatan yang baik adalah dengan melakukan investasi di bidang teknologi. Penelitian ini bertujuan untuk melakukan analisis terhadap investasi yang dilakukan oleh sebuah rumah sakit. Metode yang akan digunakan untuk menganalisis kelayakan investasi TI yaitu dilakukan menggunakan metode Information Economic (IE). Berdasarkan hasil analisis non finansial manfaat yang diperoleh yaitu adanya penghematan biaya kertas untuk laporan dan pengurangan tenaga SDM (operator), menghemat biaya penggajian karyawan, dan membantu mencapai tujuan strategis perusahaan. Selanjutnya berdasarkan hasil analisis finansial, Nilai ROI sebesar 1,00% yang menunjukan keuntungan dari investasi mencapai 1 kali lipat dari total investasi 5 tahun. Nilai NPV sebesar Rp. 9.477.162 yang menunjukan nilainya lebih dari 0 (>0) dan Nilai PP sebesar 1 bulan. Berdasarkan Dampak ekonomis dan kelayakan investasi menunjukan bahwa penghematan langsung biaya operasional selama 5 tahun sebesar Rp. 131.762.250. Sedangkan skor akhir proyek sebesar 75,01 dengan skor minimal -20 dan skor maksimal 100, sehingga menghasilkan justifikasi kategori Layak.
SISTEM PENDUKUNG KEPUTUSAN PEMILIHAN SITUS LOWONGAN KERJA MENGGUNAKAN METODE MOORA
Authors: Agustin Fajriyah Mayzura , Wijaya Indra Dharma , Harijanto Budi 
Published: August 2023

Indonesia merupakan urutan ke-10 negara yang durasi penggunaan media sosial tersering menurut (DataIndonesia.id, 2022), dengan menggunakan media sosial dapat menemukan banyak hal yang berguna seperti iklan online yang dapat menjadi sarana yang lebih efektif dalam mencari kerja. Namun terdapat juga dampak negatif bagi pencari kerja hal tersebut dibuktikan masih banyak yang mengalami penipuan lowongan kerja dalam platform yang tidak resmi sehingga tidak terjamin informasi dan keamanannya. Hal tersebut membuat para pencari kerja kuwalahan dalam memilih papan iklan (board job) lowongan kerja yang terpercaya. Berdasarkan dari permasalahan tersebut dibutuhkan sebuah Sistem Pendukung Keputusan yang dapat membantu menentukan platform resmi untuk mencari lowongan kerja bagi pencari kerja. Pada penelitian milik (Kurnianty, Ekojono, & Rohadi, 2023) tentang pendukung keputusan menggunakan metode MOORA menghasilkan akurasi sebesar 100%, dimana penggunaan metode MOORA memiliki tingkat selektifitas yang baik sehingga dapat digunakan pada penelitian ini. Metode yang digunakan yakni metode Multi-Objective Optimization On The Basis Of Ratio Analysis (MOORA) dikarenakan kriteria yang dibutuhkan terdapat beberapa kriteria yang bertentangan. Hasil yang didapatkan dengan menggunakan metode ini yakni hasil yang sesuai. Hasil Pengujian dengan membandingkan perhitungan secara manual dengan sistem menggunakan Confusion Matrix mendapatkan hasil akurasi sebesar 100%, kemudian pengujian kepada responden mendapat hasil akhir rata-rata kepuasan sebesar 89.28%, hal tersebut dapat disimpulkan bahwa sistem ini dapat memenuhi kebutuhan dan dapat membantu pencari kerja dalam memilih situs lowongan kerja.
Evaluation of a static mixer as a new microfluidic method for liposome formulation
Authors: Ota Aoba , Mochizuki Ayaka , Sou Keitaro , Takeoka Shinji 
Published: August 2023

Introduction: Microfluidic formulation of liposomes has been extensively studied as a potential replacement for batch methods, which struggle with problems in scalability and difficulty in modulating conditions. Although microfluidic devices are considered to be able to combat these issues, an adequate replacement method has yet to be established.Methods: This paper examines the potential of a static mixer (SM) by comparing the encapsulation efficiency, loading, lamellarity, and user-friendliness with a commonly used microfluidic device, a staggered herringbone micromixer (SHM).Results: In both devices, it was found that as the initial lipid concentration increased, the particle size increased; however, the overall particle size was seen to be significantly larger in the liposomes prepared with SM. PDI remained significantly smaller in SM, however, signifying that better control of the particle size was accomplished in SM. In addition, the encapsulation efficiency was slightly smaller in SM compared to SHM, and in both devices, the values increased as the initial lipid concentration increased. The increase in encapsulation efficiencies was significantly smaller than that of the theoretical encapsulation efficiency, and this was found to be due to the increase in lamellarity as the initial lipid concentration increased.Discussion: In terms of user-friendliness, SM demonstrated significant advantages. The mixing elements could be taken out from the device, allowing for thorough cleaning of the element and device before and after experiments and ensuring experiments are conducted at virgin state in every round. Consequently, it was found that SM not only can produce uniformly distributed liposomes but has the potential to become a more practical method for liposome formulation with modifications in the mixing elements.
Case report: Sodium-glucose cotransporter 2 inhibitors induce left ventricular reverse remodeling in anthracycline-related cardiac dysfunction—a case series
Authors: Giangiacomi Francesco , Faggiano Andrea , Cardinale Daniela , Rossi Francesca Gaia , Pollina Alberto , Gherbesi Elisa , Gnan Eleonora , Carugo Stefano , Vicenzi Marco 
Published: August 2023

PurposeTo describe the efficacy and safety of sodium-glucose cotransporter 2 inhibitors as a specific treatment for anthracycline-related cardiac dysfunction in a small real-world population.MethodsSeven patients with anthracycline-related cardiac dysfunction were clinically and echocardiographically evaluated before and after the introduction of sodium-glucose cotransporter 2 inhibitors.ResultsAfter a median period of 24 weeks with uninterrupted sodium-glucose cotransporter 2 inhibitors treatment, a significant clinical improvement was observed with at least one New York Heart Association Functional Class (NHYA FC) improvement in all patients (median NYHA FC: I vs. III, p &lt; 0.010). A noteworthy left ventricular reserve remodeling (median left ventricular end diastolic volume indexed: 53 vs. 82.5 ml/m2, p = 0.018; median left ventricular ejection fraction: 50% vs. 40%, p = 0.17) was also observed. Sodium-glucose cotransporter 2 inhibitors therapy was well tolerated by every patients; no cases of discontinuation or relevant side effects were observed.ConclusionSodium-glucose cotransporter 2 inhibitors induce a significant clinical improvement and left ventricular reserve remodeling in patients affected by anthracycline-related cardiac dysfunction.
Association between dietary glycemic index and non-alcoholic fatty liver disease in patients with type 2 diabetes mellitus
Authors: Salavatizadeh Marieh , Soltanieh Samira , Ataei Kachouei Amirhossein , Abdollahi Fallahi Zahra , Kord-Varkaneh Hamed , Poustchi Hossein , Mansour Asieh , Khamseh Mohammad E. , Alaei-Shahmiri Fariba , Santos Heitor O. , Hekmatdoost Azita 
Published: August 2023

ObjectiveManaging dietary glycemic index (GI) deserves further attention in the interplay between non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM). This study aimed to evaluate the relationship between dietary GI and the odds of NAFLD in patients with T2DM.MethodsA cross-sectional study was carried out between April 2021 and February 2022, including 200 participants with T2DM aged 18-70 years, of which 133 had NAFLD and 67 were in the non-NAFLD group. Cardiometabolic parameters were analyzed using standard biochemical kits and dietary intake was assessed using a validated food frequency questionnaire. Binary logistic regression was applied to explore odds ratios (ORs) and 95% confidence intervals (CIs) for NAFLD according to tertiles of dietary GI.ResultsHighest vs. lowest tertile (&lt; 57 vs. &gt; 60.89) of energy-adjusted GI was not associated with the odds of having NAFLD (OR 1.25, 95% CI = 0.6-2.57; P-trend = 0.54) in the crude model. However, there was an OR of 3.24 (95% CI = 1.03-10.15) accompanied by a significant trend (P-trend = 0.04) after full control for potential confounders (age, gender, smoking status, duration of diabetes, physical activity, waist circumference, HbA1c, triglycerides, total cholesterol, dietary intake of total carbohydrates, simple carbohydrates, fat, and protein).ConclusionHigh dietary GI is associated with increased odds of NAFLD in subjects with T2DM. However, interventional and longitudinal cohort studies are required to confirm these findings.
Mycobacterium tuberculosis PE/PPE proteins enhance the production of reactive oxygen species and formation of neutrophil extracellular traps
Authors: García-Bengoa María , Meurer Marita , Stehr Matthias , Elamin Ayssar A. , Singh Mahavir , Oehlmann Wulf , Mörgelin Matthias , von Köckritz-Blickwede Maren 
Published: August 2023

IntroductionNeutrophil granulocytes predominate in the lungs of patients infected with Mycobacterium tuberculosis (Mtb) in earlier stages of the disease. During infection, neutrophils release neutrophil extracellular traps (NETs), an antimicrobial mechanism by which a DNA-backbone spiked with antimicrobial components traps the mycobacteria. However, the specific mycobacterial factors driving NET formation remain unclear. Proteins from the proline-glutamic acid (PE)/proline-proline-glutamic acid (PPE) family are critical to Mtb pathophysiology and virulence.MethodsHere, we investigated NET induction by PE18, PPE26, and PE31 in primary human blood-derived neutrophils. Neutrophils were stimulated with the respective proteins for 3h, and NET formation was subsequently assessed using confocal fluorescence microscopy. Intracellular ROS levels and cell necrosis were estimated by flow cytometry. Additionally, the influence of phorbol-12-myristate-13-acetate (PMA), a known NADPH oxidase enhancer, on NET formation was examined. Neutrophil integrity following incubation with the PE/PPE proteins was evaluated using transmission electron microscopy.ResultsFor the first time, we report that stimulation of primary human blood-derived neutrophils with Mtb proteins PE18, PPE26, and PE31 resulted in the formation of NETs, which correlated with an increase in intracellular ROS levels. Notably, the presence of PMA further amplified this effect. Following incubation with the PE/PPE proteins, neutrophils were found to remain viable and structurally intact, as verified through transmission electron microscopy, indicating the occurrence of vital NET formation.DiscussionThese findings offer valuable insights that contribute to a better understanding of host-pathogen interactions during Mtb infection. Moreover, they underscore the significance of these particular Mtb antigens in triggering NET formation, representing a distinctive and previously unrecognized function of PE/PPE antigens.
Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326)
Authors: Ferguson Mary R. , Delgado Kristina N. , McBride Shannon , Orbe Isabel C. , La Vake Carson J. , Caimano Melissa J. , Mendez Qiana , Moraes Trevor F. , Schryvers Anthony B. , Moody M. Anthony , Radolf Justin D. , Weiner Michael P. , Hawley Kelly L. 
Published: August 2023

IntroductionSyphilis, a sexually transmitted infection caused by the spirochete Treponema pallidum (Tp), is resurging globally. Tp’s repertoire of outer membrane proteins (OMPs) includes BamA (β-barrel assembly machinery subunit A/TP0326), a bipartite protein consisting of a 16-stranded β-barrel with nine extracellular loops (ECLs) and five periplasmic POTRA (polypeptide transport-associated) domains. BamA ECL4 antisera promotes internalization of Tp by rabbit peritoneal macrophages.MethodsThree overlapping BamA ECL4 peptides and a two-stage, phage display strategy, termed “Epivolve” (for epitope evolution) were employed to generate single-chain variable fragments (scFvs). Additionally, antisera generated by immunizing mice and rabbits with BamA ECL4 displayed by a Pyrococcus furiosus thioredoxin scaffold (PfTrxBamA/ECL4). MAbs and antisera reactivities were evaluated by immunoblotting and ELISA. A comparison of murine and rabbit opsonophagocytosis assays was conducted to evaluate the functional ability of the Abs (e.g., opsonization) and validate the mouse assay. Sera from Tp-infected mice (MSS) and rabbits (IRS) were evaluated for ECL4-specific Abs using PfTrxBamA/ECL4 and overlapping ECL4 peptides in immunoblotting and ELISA assays.ResultsEach of the five mAbs demonstrated reactivity by immunoblotting and ELISA to nanogram amounts of PfTrxBamA/ECL4. One mAb, containing a unique amino acid sequence in both the light and heavy chains, showed activity in the murine opsonophagocytosis assay. Mice and rabbits hyperimmunized with PfTrxBamA/ECL4 produced opsonic antisera that strongly recognized the ECL presented in a heterologous scaffold and overlapping ECL4 peptides, including S2. In contrast, Abs generated during Tp infection of mice and rabbits poorly recognized the peptides, indicating that S2 contains a subdominant epitope.DiscussionEpivolve produced mAbs target subdominant opsonic epitopes in BamA ECL4, a top syphilis vaccine candidate. The murine opsonophagocytosis assay can serve as an alternative model to investigate the opsonic potential of vaccinogens. Detailed characterization of BamA ECL4-specific Abs provided a means to dissect Ab responses elicited by Tp infection.
KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors
Authors: Mac Donald Alice , Guipouy Delphine , Lemieux William , Harvey Mario , Bordeleau Louis-Jean , Guay David , Roméro Hugo , Li Yuanyi , Dion Renaud , Béland Kathie , Haddad Elie 
Published: August 2023

IntroductionNatural Killer (NK) cells hold the potential to shift cell therapy from a complex autologous option to a universal off-the-shelf one. Although NK cells have demonstrated efficacy and safety in the treatment of leukemia, the limited efficacy of NK cell-based immunotherapies against solid tumors still represents a major hurdle. In the immunosuppressive tumor microenvironment (TME), inhibitory interactions between cancer and immune cells impair antitumoral immunity. KLRC1 gene encodes the NK cell inhibitory receptor NKG2A, which is a potent NK cell immune checkpoint. NKG2A specifically binds HLA-E, a non-classical HLA class I molecule frequently overexpressed in tumors, leading to the transmission of inhibitory signals that strongly impair NK cell function.MethodsTo restore NK cell cytotoxicity against HLA-E+ tumors, we have targeted the NKG2A/HLA-E immune checkpoint by using a CRISPR-mediated KLRC1 gene editing.ResultsKLRC1 knockout resulted in a reduction of 81% of NKG2A+ cell frequency in ex vivo expanded human NK cells post-cell sorting. In vitro, the overexpression of HLA-E by tumor cells significantly inhibited wild-type (WT) NK cell cytotoxicity with p-values ranging from 0.0071 to 0.0473 depending on tumor cell lines. In contrast, KLRC1KO NK cells exhibited significantly higher cytotoxicity when compared to WT NK cells against four different HLA-E+ solid tumor cell lines, with p-values ranging from&lt;0.0001 to 0.0154. Interestingly, a proportion of 43.5% to 60.2% of NKG2A− NK cells within the edited NK cell population was sufficient to reverse at its maximum the HLA-E-mediated inhibition of NK cell cytotoxicity. The expression of the activating receptor NKG2C was increased in KLRC1KO NK cells and contributed to the improved NK cell cytotoxicity against HLA-E+ tumors. In vivo, the adoptive transfer of human KLRC1KO NK cells significantly delayed tumor progression and increased survival in a xenogeneic mouse model of HLA-E+ metastatic breast cancer, as compared to WT NK cells (p = 0.0015).ConclusionsOur results demonstrate that KLRC1 knockout is an effective strategy to improve NK cell antitumor activity against HLA-E+ tumors and could be applied in the development of NK cell therapy for solid tumors.
Comparison of clinical outcomes in critical patients undergoing different mechanical ventilation modes: a systematic review and network meta-analysis
Authors: Wu Mengyu , Zhang Xiaohong , Jiang Yu , Guo Yun , Zhang Wenjing , He Hong , Yin Yanhua 
Published: August 2023

PurposeTo evaluate the effects of different mechanical ventilation modes on critical patients.MethodsPubMed, Embase, Web of science, and Cochrane Library databases were searched from their inception to November 15, 2022 for randomized controlled trials on the application of different mechanical ventilation modes in critical patients. Two researchers independently screened the literature, extracted data, and assessed the risk of bias in the included studies. R4.2.1 was used for this network meta-analysis.ResultsTwenty-eight RCTs involving 3,189 patients were included. The interventions in these RCTs included NAVA (neurally adjusted ventilatory assist), PAV (proportional assist ventilation), ASV (adaptive support ventilation), Smartcare/PS (Smartcare/pressure support), PSV (pressure support ventilation), PSV_ATC (pressure support ventilation_automatic tube compensation), and SIMV (synchronized intermittent mandatory ventilation). The network meta-analysis showed that, compared with the PSV group, there was no significant difference in duration of mechanical ventilation, duration of ICU stay, and hospital stay between NAVA, SIMV, AVS, PAV, Smartcare/PS, and PSV_ATC groups. Compared with PSV, PAV improved the success rate of withdrawal of ventilator [OR = 3.07, 95%CI (1.21, 8.52)]. Compared with PSV and PAV, NAVA reduced mortality in the ICU [OR = 0.63, 95%CI (0.43, 0.93); OR = 0.45, 95%CI (0.21, 0.97)].ConclusionNAVA can reduce mortality in ICU, and PAV may increase the risk of withdrawal of the ventilator. There was no significant difference between PSV and other mechanical ventilation modes (NAVA, SIMV, AVS, PAV, Smartcare/PS, and PSV_ATC) in the duration of mechanical ventilation, duration of ICU stay, or hospital stay. Due to the limitations, more high-quality studies are needed to verify these findings.
Minimizing blood loss in laparoscopic myomectomy with temporary occlusion of the hypogastric artery
Authors: Balulescu Ligia , Nistor Samuel , Lungeanu Diana , Brasoveanu Simona , Pirtea Marilena , Secosan Cristina , Grigoras Dorin , Caprariu Radu , Pasquini Andrea , Pirtea Laurentiu 
Published: August 2023

IntroductionUterine leiomyomas are common benign pelvic tumors. Currently, laparoscopic myomectomy (LM) is the preferred treatment option for women in the fertile age group with symptomatic myomas. The authors hypothesize that combining LM with a bilateral temporary occlusion of the hypogastric artery (TOHA) using vascular clips minimizes uterine blood flow during surgery and can significantly reduce surgery-associated blood loss.Materials and methodsThis single-center, prospective randomized study was conducted at the Department of Obstetrics and Gynecology, Municipal Emergency Clinical Hospital Timisoara, Romania. Patients aged between 18 and 49 who preferred laparoscopic myomectomy and wished to preserve fertility were included, provided they had intramural uterine leiomyomas larger than 4 cm in diameter that deformed the uterine cavity. The study analyzed data from 60 laparoscopic myomectomies performed by a single surgeon between January 2018 and December 2020. Patients were randomly assigned to either: “LM + TOHA” group (29 patients), and “LM” group (31 patients). The study’s main objective was to evaluate the impact of TOHA on perioperative blood loss, expressed as mean differences in Hb (delta Hb).ResultsDelta Hb was statistically lower in the “LM + TOHA” group compared to “LM” group, with mean ± standard (min–max): 1.68 ± 0.67 (0.39–3.99) vs. 2.63 ± 1.06 (0.83–4.92) g/dL, respectively (p &lt; 0.001). There was a statistically significant higher need for postoperative iron perfusion in the “LM” group, specifically 0 vs. 12 patients (p &lt; 0.001), and lower postoperative anemia in “LM + TOHA” group (p &lt; 0.001). Necessary artery clipping time was 10.62 ± 2.47 (7–15) minutes, with no significant impact on overall operative time: 110.2 ± 13.65 vs. 106.3 ± 16.48 (p = 0.21). There was no difference in the length of hospitalization or 12-month post-intervention fertility.DiscussionPerforming bilateral TOHA prior to laparoscopic myomectomy has proven to be a valuable technique in reducing surgery-associated blood loss, while minimizing complications during surgery, with no significant increase in the overall operative time.Clinical trial registrationISRCTN registry, (www.isrctn.com), identifier ISRCTN66897343.