bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pelatihan Pembuatan MP-ASI Berbasis Pangan Lokal Di Desa Domas, Kabupaten Gresik
Authors: Oktaviani Diani , Suprapti Suprapti , Mulyani Endah 
Published: September 2023

Kesehatan anak khusunya bayi dan balita merupakan salah satu aspek prioritas untuk diperhatikan karena seorang anak memiliki nilai yang sangat tinggi pada anggota keluarga dan bangsanya. Setiap orang tua mengingin­kan dan mengharapkan buah hati mereka dapat tumbuh dan berkembang secara optimal, dengan memberikan nutrisi pada dua tahun kehidupanya, yaitu pemberian MP-ASI selain ASI. Di Desa Domas adanya keterbatasnya pengetahuan ibu tentang gizi dan keterampilan kader dan ibu bayi dalam menyiapkan MP - ASI untuk memenuhi kebutuhan gizi balita dengan memanfaatkan sumberdaya pangan lokal yang tersedia. Tujuan pengandian masyarakat ini untuk membantu kader dan ibu balita dapat mengetahui dan mengelola MP – ASI sesuai dengan usia anak, dan mengetahui resep makanan anak yang bersumber dari bahan pangkal local. Hasil dari pengabdian masyarakat ini adalah kegiatan ini berjalan dengan lancer, dihadiri 25 ibu yang terdiri dari kader dan ibu balita. 25 ibu (100%) tersebut dapat memahami dan dapat melakukan pembuatan / penggolaan MP-ASI dengan baik dan benar.
Kata kunci Pelatihan, MP-ASI, Pangan Lokal
Pelatihan Pembelajaran Interaktif untuk Pelaksana Kelas Persiapan Pernikahan di Kecamatan Sambi Boyolali
Authors: Linadi Kinanthi Estu 
Published: September 2023

Kasus perceraian di Indonesia masih mengalami peningkatan dalam lima tahun terakhir. Pada tahun 2020, BPS mencatat persentase perceraian mengalami kenaikan dari tahun sebelumnya, yaitu 4,7 juta pasangan. Sedangkan menurut data Dirjen Dukcapil, data cerai hidup hingga pertengahan tahun 2021 mencapai 3,97 juta pasangan. Tingginya angka perceraian tersebut disebabkan oleh banyak faktor seperti masalah ekonomi, isu kesetiaan, termasuk ketidaksiapan membina rumah tangga. Sebagian besar calon pengantin di Indonesia menikah tanpa membekali diri dengan pengetahuan yang cukup tentang pernikahan. Kementerian Agama terus berupaya menekan angka perceraian dengan mewajibkan calon pengantin untuk mengikuti kelas persiapan pernikahan sebagai syarat mendaftarkan pernikahan. Di Kecamatan Sambi, kelas persiapan pernikahan dilaksanakan oleh Puskesmas dan KUA. Metode yang selalu digunakan dalam kelas persiapan pernikahan adalah ceramah sehingga peserta cenderung bosan dan kurang bersemangat. Sedangkan, agar tujuan pendidikan kesehatan dapat optimal, diperlukan metode yang dapat melibatkan sebanyak mungkin indera. Untuk itu, perlu dilakukan Pelatihan Metode Pembelajaran Interaktif dengan Kartu Permainan untuk Pelaksana Kelas Persiapan Pernikahan di Kecamatan Sambi Kabupaten Boyolali. Tujuan kegiatan pengabdian kepada masyarakat ini adalah meningkatkan kemampuan Pelaksana Kelas Persiapan Pernikahan dalam menyampaikan materi di Kelas Persiapan Pernikahan. Setelah mengikuti pelatihan, para pelaksana Kelas Persiapan Pernikahan berkomitmen untuk menerapkan metode pembelajaran interaktif yang telah dipelajari.
Kata Kunci: Kelas Persiapan Pernikahan, Pelatihan
HUBUNGAN PERAWATAN VULVA HYGIENE PADA WANITA USIA SUBUR DENGAN KEJADIAN FLOUR ALBUS DI DESA MATTIROWALIE KECAMATAN TANETE RIAJA KABUPATEN BARRU
Authors: Hasriani St. , Pratiwi Wilda Rezki , Asnuddin Asnuddin , Syamson Meriem Meisyaroh , Bunyanis Fitriana 
Published: September 2023

Perubahan secara fisik pada organ reproduksi wanita usia subur dipengaruhi oleh pematangan hormon seksual. Terjadi peningkatan hormone estrogen dan progesteron menyebabkan perubahan seks sekunder diantaranya perubahan postur tubuh, pembesaran payudara, pertumbuhan rambut kelamin dan aksila serta mulai terjadi menstruasi.Tujuan dalam penelitian ini adalah untuk mengetahui hubungan perawatan vulva hygiene pada wanita usia subur dengan kejadian flour albus di Desa Mattirowalie Kecamatan Tanete Riaja Kabupaten Barru. Jenis penelitian ini adalah metode observasional dengan pendekatan Cross Sectional Study. Populasi adalah semua wanita usia subur yang berada di Desa Mattirowalie Kecamatan Tanete Riaja Kabupaten Barru sebanyak 1.041 orang. Sampel adalah wanita usia subur yang berada di Desa Mattirowalie Kecamatan Tanete Riaja Kabupaten Barru sebanyak 91 orang dengan menggunakan teknik pengambilan sampel secara Purposive Sampling. Hasil penelitian menunjukkan bahwa dari 91 orang yang dijadikan sampel, yang mengalami vulva hygiene kurang sebanyak 13 orang (14,3%) dan vulva hygiene baik sebanyak 78 orang (85,7%). Hasil penelitian menunjukkan bahwa dari 91 orang yang dijadikan sampel, yang mengalami flour albus tidak normal sebanyak 14 orang (15,4%) dan flour albus normal sebanyak 77 orang (84,6%). Hasil penelitian menunjukkan bahwa terdapat hubungan signifikan perawatan vulva hygiene pada wanita usia subur dengan kejadian flour albus di Desa Mattirowalie Kecamatan Tanete Riaja Kabupaten Barru
The EFFECT OF PARENTS' SUPPORT WITH ANXIETY LEVELS OF MENARCHE IN ADOLESCENT WOMEN AT SDN MURTAJIH 1 PADEMAWU, PAMEKASAN
Authors: Zulaikha Layla Imroatu , Oktarina Sarindani , Damayanti Tri Yunita Fitria 
Published: September 2023

Menarchea merupakan perdarahan pertama dari uterus yang terjadi pada seorang wanita. Menarche biasa terjadi pada usia 10-16 tahun, rata-rata 12,5 tahun Banyak remaja yang merasa cemas pada saat akan menghadapi menarche yang ditandai rasa malu dan takut, dengan tingkat kecemasan yang bervariasi. Berdasarkan studi pendahuluan diperoleh data 60% remaja putrid tersebut tidak mendapatkan dukungan dari orang tuakhususnyadukungan yang bersifatinformatifberkenaandengan menstruasi. Tujuan penelitian ini adalah untuk mengetahui hubungan dukungan orang tuadengan tingkat kecemasan menghadapi menarche pada remaja putri.
Penelitian ini merupakan penelitian analitik korelasional dengan pendekatan cross sectional. Populasinya adalah seluruh siswi Sekolah Dasar Murtajih 1 dengan teknik pengambilan sampel adalah random sampling sebanyak 71 responden. Pengumpulan data dalam penelitian ini dengan menggunakan kuesioner dan cheklist. Teknik analisa data menggunakan uji statistik spearman rank melalui bantuan SPSS versi 17,0 dengan tingkat signifikansi 0,05.
Dari hasil uji statistik spearman rank diperoleh bahwa ρ : 0,000<0,05 yang berarti H0 ditolak yang berarti ada hubungan antara dukungan orang tua dengan tingkat kecemasan menghadapi menarche pada remaja putri.
Dukungan orang tua merupakan suatu elemen penting dalam membentuk persepsi pada remaja. Dukungan orang tua yang baik akan membantu mengurangi tingkat kecemasan menghadapi menarche pada remaja putri. Karena dengan begitu, remaja putri mendapatkan informasi khususnya mengenai menstruasi yang akan memberi persepsi yang positif mengenai menarche. Hal tersebut diperlukan kesadaran orang tua sebagai pendidik pertama dan utama bagi anaknya.
Modes of Presentation and Patterns of Coronary Artery Disease in Patients Undergoing Percutaneous Intervention at Birat Medical College Teaching Hospital
Authors: Shah Prashant , Bista Madhab , KC Heera 
Published: September 2023

Background and Aims: Percutaneous coronary interventions have been provided in various hospitals of Nepal for more than two decades. Hence, we aimed to study the percutaneous coronary intervention revascularization status in patients with coronary artery disease at a tertiary center of eastern Nepal.
Methods: A cross sectional study was conducted at Birat Medical College Teaching Hospital from 1st Aug to 30th Feb 2023. Ninety seven coronary artery disease patients (Unstable angina, Non ST elevated Myocardial Infarction ST elevated Myocardial Infarction and Stable Angina) requiring percutaneous coronary interventions were enrolled. Patient socio-demography and findings of percutaneous coronary intervention were recorded, entered in Microsoft Excel and analyzed by SPSS 23.
Results: Majority 30(31.3%) had unstable angina. Left main coronary artery was normal in all patients. Severe stenosis was found in 58(59.7%) of left anterior descending artery, 32(32.9%) of right coronary artery and 29(29.8%) of left circumflex artery. Total occlusion was found in 10(10.3%) of left anterior descending artery, 8(8.2%) of right coronary artery and 6(6.2%) of left circumflex artery. Majority 68(70.1%) of patients had elective percutaneous coronary intervention.
Conclusion: The most common mode of presentation of coronary artery disease was unstable angina and majority have severe stenosis. The preferred intervention was elective percutaneous coronary intervention.
Predictive Value of EuroSCORE II in a Nepalese Tertiary University Hospital – Prospective Observational Study
Authors: Bashyal Krishnaprasad , Baral Ravi Kumar , Bhattarai Anil , Khakural Prabhat , Kadel Prashiddha B. , Koirala Bhagawan 
Published: September 2023

Background and Aims: The European System for Cardiac Operative Risk Evaluation (EuroSCORE) is an important tool for risk stratification in cardiac surgery. Updated EuroSCORE II (ESC II) is widely regarded as essential for risk stratification and several studies validate its efficacy, but these are fewer in Southern Asia and none in Nepal. Aim of this study was to compare the predicted versus observed early mortality in adults undergoing major cardiac surgeries in Nepalese population.
Methods: A prospective observational study was conducted from September 2019 to May 2021 at Manmohan Cardiothoracic Vascular and Transplant Center. Calibration and discrimination of scoring system were main parameters analyzed in total sample and subgroups. Discrimination were observed by plotting receiver operating characteristic (ROC) curves and calculating area under curve (AUC). Two sample t test, McNemar’s test, Fisher’s exact t test and Chi square test were used to derive P value.
Results: In this study, 249 patients were evaluated. Poor mortality prediction was shown by statistically significant p value <0.05 across all surgical groups. AUC for total cases, CABG and valve surgeries were 0.835, 0.766, 0.82 respectively.
Conclusion The present study underpredicted mortality but displayed good discrimination for overall cardiac surgeries, with excellent discrimination in valve surgeries. As current sample is not entirely comparable to parent study, weak calibration could be attributed to it as etiology was mostly rheumatic with poorer cardiopulmonary reserve in this study versus degenerative etiology in original study.
Uricosuric effect of dandelion root extract on oxonate-induced hyperuricemia in rats
Authors: Khallawi Khairullah M. , Hameed Basim J. , Neamah Nadheerah F. 
Published: September 2023

This research aimed to investigate the uricosuric effect of dandelion plant extracts in hyperuricemic rats induced by potassium oxonate (PO).
Methods. Hyperuricemia was induced in rats using PO, and dandelion root extracts were administered to observe their impact on uric acid (UA) levels. The study involved adult male Swiss rats weighing approximately 150–180 grams, randomly divided into five groups (n = 6). Group 1 served as the normal control group with no treatment. Group 2 received PO only. Group 3 received oral administration of 50 mg/kg of dandelion extract in 0.5 ml of distilled water (DW) daily. Group 4 was orally administered 100 mg/kg of dandelion powder in 0.5 ml of DW daily. Group 5 was orally treated with allopurinol.
After 12 days, the rats were euthanized using chloroform inhalation, and their sera were collected directly from the heart for biochemical analysis of serum UA, urinary uric acid (UUA), as well as other liver and renal biochemical parameters.
Results. The study revealed that hyperuricemic rats treated with the dandelion solution experienced a significant decrease in blood UA levels and a significant increase in UUA levels. Dandelion treatment also influenced xanthine oxidase activity, with no significant differences observed in liver and kidney functions.
Conclusion. Based on the findings of this study, it can be concluded that dandelion extract significantly reduces UA levels through uricosuric activity and demonstrates significant XO inhibitory effects.
Comparison of sleep quality in patients undergoing hemodialysis and renal transplant recipients
Authors: Parmaksız Ergün , Parmaksız Elif Torun 
Published: September 2023

Low sleep quality is a prevalent health issue among patients undergoing kidney replacement therapy. Our objective was to assess and compare sleep quality between patients undergoing hemodialysis (HD) and kidney transplant recipients.
Methods. This observational, cross-sectional study recorded socio-demographic data and medical histories. The study comprised two groups: patients undergoing HD for at least one year and kidney transplant recipients with a successful renal transplant over six months. Each participant completed the Pittsburgh Sleep Quality Index (PSQI).
Results. The study encompassed 56 HD patients and 35 age and gender-matched renal transplant recipients. The mean age of the entire study population was 47.97±12.92 years (ranging from 23 to 77), with 49 (53.8%) being males. PSQI scores were ≥5 in 57 patients, including 21 transplant recipients and 36 undergoing HD patients. PSQI results exhibited no significant difference between the transplant and HD groups. The mean sleep quality score was 5.69±2.95 in the transplant group and 5.72±3.29 in the HD group, with no statistically significant difference.
Conclusions. PSQI scores were similar in patients undergoing HD and transplant recipients with well-preserved renal functions. Identifying low sleep quality is essential for enhancing the overall quality of life.
Correction of the prooxidant-antioxidant balance disorders in patients with diabetic kidney disease
Authors: Nesen A. , Semenovykh P. , Galchiskaya V. , Yakymenko Yu. , Chernyshov V. 
Published: September 2023

The present study aimed to evaluate the effect of sodium-glucose co-transporter 2 (SGLT2) inhibitor dapagliflozin therapy on the prooxidant-antioxidant balance (PAB) in patients with diabetic kidney disease.
Methods. A total of 88 patients with type 2 diabetes mellitus (DM) and diabetic nephropathy (DN) were included in this single-center randomized open-label prospective study. All patients were randomly divided into 2 groups: 45 patients received a standard course of treatment, which included antidiabetic drugs, renin-angiotensin-aldosterone system blockers, and HMG-CoA reductase inhibitors (statins). In addition to the standard therapy, the remaining 43 patients were prescribed the SGLT2 inhibitor dapagliflozin 10 mg per day. Patients were re-examined after 6 months of treatment. The blood PAB was calculated as the ratio of total hydroperoxides (THP) to total antioxidant activity (TAA). The level of THP and TAA was determined by the colorimetric method.
Results. PAB was significantly elevated in type 2 DM patients with nephropathy due to TAA decrease and THP level increase when compared to the control group. The highest values of PAB were found in the late stages of DN in patients with glomerular filtration rates <60 ml/min/1.73m2. In patients who received dapagliflozin, significant PAB elevation by 30,55% (р < 0.05) was observed as well as THP decrease and TAA increase in blood. In the standard therapy group, no significant changes in PAB parameters were detected.
Conclusions. Add-on treatment with dapagliflozin resulted in a more significant improvement of the PAB in patients with DN in comparison with standard treatment.
Assay of Diphtheria Vaccine Potency by Intradermal Challenge Test
Authors: Komarovskaya E. I. , Perelygyna О. V. 
Published: September 2023

Relevance. Modern identifying potency (immunogenicity) of diphtheria toxoid tests are based on determining immunized animals resistance for administration challenge toxin or evaluation of protective antibodies level in serum. In Russia to assess the potency of diphtheria toxoid (DT) the challenge lethal method has been used for more than 60 years, challenge is based on determination of potency via its possibility to defend immunized animals from lethal doses of diphtheria toxin. This method is used as «golden standard». Last decade European Union Regulatory politic is headed to reduce of using animals in pharmaceutical practice, "The Three Rs" approach. In frameworks of this approach alternative methods of evaluating immunogenicity DT were purposed, particularly, intradermal challenge method. Within this test the potency of diphtheria toxoid is evaluated by its possibility to protect immunized animals from intradermal administration of diphtheria toxin. In the production of a number of foreign vaccines intradermal challenge test is used for assessing potency.Aim. Comparative characteristics of methods of lethal challenge test and intradermal challenge tests to determine the potency of diphtheria toxoid in combined vaccines and toxoids made in Russia.Materials and methods. Materials of domestic and foreign manufacturers were used in the work: combined vaccines for diphtheria prevention, diphtheria toxins, reference-vaccine. The determination of the potency of diphtheria toxoid was carried out on guinea pigs by pharmacopoeias methods: lethal challenge test and intradermal challenge tests.Results. To assess the potency of diphtheria toxoid using, a technique was used in accordance with WHO recommendations; for this, guinea pigs similar in sensitivity to diphtheria toxin to Dunkin Hartley pigs were selected. The suitability of the diphtheria toxin used in Russia for determining the potency of diphtheria toxoid by the method of intradermal challenge test was assessed when evaluating the effectiveness of vaccines for the prevention of diphtheria. Comparative tests of the intradermal challenge tests and lethal challenge tests were carried out to determine the potency of diphtheria toxoid.Conclusions. The conducted studies have demonstrated the fundamental possibility of using the intradermal challenge tests to assess the potency of diphtheria toxoid. However, for a number of factors, such as the variability of results, the number of animals, etc., the intradermal challenge tests is inferior to the method of lethal challenge tests in assessing the potency of diphtheria vaccines in routine practice.
Influenza and Pneumococcal Vaccination of Healthcare Workers during a Pandemic Reduces the Risk and Severity of COVID-19 in Vaccinated
Authors: Kostinov M. P. , Nastaeva N. Yu. , Vlasenko A. E. , Kostinova A. M. , Mashilov K. V. , Simonova E. G. 
Published: September 2023

Background. Individuals who were vaccinated against seasonal influenza or had a history of pneumococcal vaccination were found to be less likely to become infected and tolerate COVID-19 more easily. However, it has not been sufficiently studied how vaccination against these infections, carried out during the pandemic period, can affect the incidence of COVID-19.Aims. The purpose of the investigation: to study the effect of vaccination against influenza and pneumococcal infection carried out during the pandemic of a new coronavirus infection on the susceptibility and course of COVID-19 in healthcare workers.Materials and methods. In August- Setempber 2020, after the first rise in the incidence of COVID-19, out of 547 employees (aged 18 to 70 years) of a medical organization (MO), 266 (49%) were vaccinated against influenza (group II, n = 98), pneumococcal infection (group III, n = 60) and combined vaccination (group IV, n = 108), while 281 (51%) remained unvaccinated (group 1). Follow-up period: from September 2020 to March 2021 with the registration of the incidence of acute respiratory infections (ARI) according to primary medical records and the use of PCR methods for SARS-CoV-2, epidemiological and statistical analysis.Results. Two months after the start of the study, the proportion of cases of COVID-19 in the 1st group (unvaccinated) was 5% versus 1% in the 4th group (persons vaccinated with two vaccines), after 4 months – 15% and 5%, respectively, and at the end of observation (166 days) – 16% and 8%, respectively. That is, among unvaccinated individuals, the risk of getting COVID-19 was higher by HR = 2.1 [95% CI: 1.0÷4.7] times. The time between the start of observation and a positive test for COVID-19 in study participants was significantly higher in the 4th group compared to the group I: 106 [60–136] days versus 47 [17–75] days. The distribution of patients with COVID-19 according to the severity of viral pneumonia showed that in unvaccinated patients in most (64%) cases, pneumonia had a moderate to severe course, while in the 4th group of patients with combined vaccination in 100% of cases, mild (p = 0.04 for the entire sample).Conclusions. During the COVID-19 epidemic rises, vaccination against respiratory infections remains relevant, reducing the number of cases, the severity of the coronavirus infection and preventing the occurrence of co-infections.
Development of a Reagent Kit for the Quantitative Determination of Hepatitis B Virus (HBV) DNA in Clinical Material by PCR with Hybridization-Fluorescence Detection
Authors: Zhigaleva O. N. , Mardanly S. G. , Gashenko T. Yu. , Ermolaev I. I. 
Published: September 2023

Relevance.Hepatitis B virus (HBV) is one of the most common viral infections affecting people worldwide and can lead to chronic hepatitis, cirrhosis and hepatocellular carcinoma. Currently 3% of the world's population are infected with hepatitis B virus and are at risk of developing life-threatening liver disease. Immunological and molecular biological methods of detection of HBV are currently used in laboratory diagnostics. The polymerase chain reaction (PCR) is currently the most sensitive method for the detection and quantification of HBV. HBV DNA quantification is widely used to monitor the antiviral treatment of HBV infection.Aim.To develop a real-time PCR kit for the quantification of HBV DNA.Materials and methods.A total of 200 plasma and serum samples positive and negative for HBV were used in the development. The performance of the developed kit was compared with the use of other commercially registered HBV diagnostic kits in Russia. Additionally, the nucleotide sequences of all existing virus genotypes analysed for the selection of primers using GeneBank system.Results and discussion.Comparison analysis of the results of quantitative determination by real-time PCR in 200 clinical serum and blood plasma samples showed that the diagnostic sensitivity of the developed kit was 100% and specificity 100%. The primers developed specific to the POL gene region. The kit is capable of detecting all types of virus genotypes.Conclusions.The developed reagent kit allows detection of hepatitis B virus and determination of its quantity within 70 minutes. In addition to a large number of genotypes and subgenotypes, the virus is characterized by mutational changes in the genome, which complicates its diagnosis and, as a consequence, the ongoing therapy with drugs. Conservative regions for primer and probe selection taken into account in the development, and the sequencing results obtained are applicable to all HBV genotypes. The reagent kit is designed to monitor HBV infected patients and will allow the analysis of different HBV viral loads.