bluuebunny/crossref_metadata_2025_split huggingface dataset:
Method development and validation for analysis of microencapsulated cyanidin‐3‐O‐rutinoside in dairy samples containing juçara palm fruit by high‐performance liquid chromatography
Authors: Tunin Luana Magri , de Paula Mariana Nascimento , Matioli Graciette , de Medeiros Araújo Daniela Cristina , Novello Cláudio Roberto , Ferreira Emilene Dias Fiuza , de Mello João Carlos Palazzo 
Published: August 2023

AbstractEuterpe edulis is a plant native of Brazil and popularly known as juçara. The aim of this study was to develop and validate an analytical method using high‐performance liquid chromatography (HPLC) of juçara crude extracts (JCEs) and to evaluate dairy products containing free and microencapsulated extracts. The validated analytical method for JCE was performed using cyanidin‐3‐O‐rutinoside (C3R – isolated and identified as major component from JCE) as a reference: linearity was obtained using ten concentrations between 25 and 250 μg mL−1 (R2 = 0.994); limits of detection and quantification were 19.04 μg mL−1 and 57.7 μg mL−1 respectively; accuracy (relative standard deviation) was <5%; recovery was 104.85 μg mL−1; and the method is robust. Physicochemical analyses of the dairy products were evaluated using colour, pH, and concentration of anthocyanins using HPLC calculated as C3R over 21 days of storage. The pH remained stable until the end of the test. The colour and the concentration of anthocyanins remained stable for 9 days. C3R was isolated, the HPLC method was developed and validated, and the colour stability was not altered until 9 days. © 2023 Society of Chemical Industry.
An efficient approach addressing the chemical complexity of Jiawei Fangji Huangqi decoction by integrating ultra‐high‐performance liquid chromatography/ion mobility‐quadrupole time‐of‐flight mass spectrometry and intelligent data processing workflows
Authors: Jiang Meiting , Zhao Dongxue , Zou Yadan , Li Xiaohang , Lou Jia , Wang Yu , Gao Xiumei , Yang Wenzhi 
Published: August 2023

A challenge in the quality control of traditional Chinese medicine is the systematic multicomponent characterization of the compound formulae. Jiawei Fangji Huangqi, a modified form of Fangji Huangqi, is a prescription comprising seven herbal medicines. To address the chemical complexity of the Jiawei Fangji Huangqi decoction, we integrated ion mobility‐quadrupole time‐of‐flight high‐definition MSE coupled to ultra‐high‐performance liquid chromatography and intelligent data processing workflows available in the UNIFI software package. Good chromatographic separation was achieved on CORTECS UPLC T3 column within 52 min, and high‐accuracy MS2 data were acquired using high‐definition MSE in the negative and positive modes. A chemical library of 1250 compounds was created and incorporated into the UNIFI software to enable automatic peak annotation of the high‐definition MSE data. We identified or tentatively characterize 430 compounds in the Jiawei Fangji Huangqi decoction. The potential superiority of high‐definition MSE over conventional MS data acquisition approaches was revealed in its spectral quality (MS2), differentiation of isomers, separation of coeluting compounds, and target mass coverage. The multiple components of the Jiawei Fangji Huangqi decoction were elucidated, offering insight into its improved pharmacological action compared with that of the Fangji Huangqi formula.
How Often is Cancer Present in Oral Cavity Re‐resections After Initial Positive Margins?
Authors: Prasad Kavita , Sharma Rahul , Habib Daniel , Sinard Robert , Mannion Kyle , Rohde Sarah , Langerman Alexander , Netterville James , Rosenthal Eben , Lewis James , Topf Michael C. 
Published: August 2023

ObjectiveTo evaluate the rate at which carcinoma is present in the re‐resection specimen following initial positive margins during head and neck cancer surgery and its impact on oncologic outcomes.Study DesignRetrospective chart review.MethodsA single institution retrospective chart review of patients that underwent curative‐intent surgery for oral cavity cancer was performed. Final pathology reports were reviewed to identify patients with initial positive margins who underwent re‐resection during the same operation. Initial positive margin was defined as severe dysplasia, carcinoma in situ (CIS), or carcinoma. Cox proportional hazards and Kaplan–Meier analyses were used to assess for associations with survival outcomes.ResultsAmong 1873 total patients, 190 patients (10.1%) had initial positive margins and underwent re‐resection during the same surgery. Additional carcinoma, CIS, or severe dysplasia was found in 29% of re‐resections, and 31% of patients with initial positive margins had final positive margins. Half of the patients with a final positive margin had a positive margin at an anatomic site different than the initial positive margin that was re‐resected. The median follow‐up was 636 days (range 230–1537). Re‐resection with cancer and final positive margin status was associated with worse overall survival (OS; p = 0.044 and p = 0.05, respectively). However, only age, T4 disease, and surgery for recurrent oral cavity cancer were independently associated with OS (p < 0.001, p = 0.005, and p = 0.001, respectively).ConclusionsFewer than a third of oral cavity re‐resections contain further malignancy, which may suggest that surgeons have difficulty relocating the site of initial positive margin. Final positive margins are often at anatomic sites different than the initial positive margin.Level of Evidence4 Laryngoscope, 134:717–724, 2024
Vestibular Preservation in Pediatric Cochlear Implantation
Authors: Licameli Greg R. , Wang Alicia , Zhou Guangwei , Faller David , Kenna Margaret , Poe Dennis , Shearer Eliot , Oster Linda , Brodsky Jacob R. 
Published: August 2023

ObjectivesEvaluate the rate of preserved vestibular function in pediatric cochlear implant surgery.Study DesignRetrospective case review.MethodsPre‐ and post‐operative vestibular tests were compared in children who underwent cochlear implantation at a tertiary level pediatric hospital over a 4‐year period.ResultsData from 59 implanted ears in 44 children was included. Median age was 2.8 years at initial testing (range 7 months – 21 years) with 1:1 male/female ratio. Implant surgeries were 26 unilateral, 13 bilateral simultaneous, and 5 bilateral sequential. The majority were implanted with slim, non‐styletted electrodes (86.4%) via a round window approach (91.5%). Normal pre‐operative results were preserved post‐operatively on rotary chair testing in 75% (21/28) of patients, cervical vestibular evoked myogenic potential testing in (75%) 30/40 of ears tested, ocular vestibular evoked myogenic potential testing in 85.7% (6/7) of ears tested, video head impulse testing in 100% (9/9) of ears tested, and computerized dynamic posturography in 100% (5/5) of patients tested. Overall, 62.5% of patients had no new deficits on any vestibular test performed post‐operatively.ConclusionsPreservation rates of vestibular function following cochlear implant surgery were higher in this cohort than what has been reported in many earlier studies. Contemporary, less traumatic electrodes and insertion techniques may be a significant factor. The risk of causing a new, severe bilateral vestibular loss with long‐term functional impacts appears to be low. Further study is warranted on the impacts of different cochlear implant electrode designs and insertion approaches on post‐operative vestibular preservation.Level of Evidence4, Case Series Laryngoscope, 134:1913–1918, 2024
Assessment of bioactive compounds in faba bean using infrared spectroscopy
Authors: Johnson Joel B. , Walsh Kerry B. , Naiker Mani 
Published: August 2023

AbstractFaba bean (Vicia faba) is growing in popularity in Australia, partly due to its higher levels of health‐benefiting compounds compared to other grain crops. This study investigated infrared spectroscopy for predicting levels of bioactive compounds such as antioxidants and phenolics in faba bean flour. Calibration models were performed on 60 samples of faba bean, comprising 10 varieties grown across two field locations in 1 year. For model validation, an independent test set comprising the same varieties grown in a different year was utilised. Near‐infrared spectroscopy (NIRS) showed promise for the prediction of total phenolic content, with an R2pred of 0.66 and root mean square error of prediction (RMSEP) of 76 mg/100 g. Similarly, prediction of ferric reducing antioxidant power, a measure of antioxidant activity, gave an R2pred of 0.59 and RMSEP of 87 mg/100 g. Additionally, moving window optimisation was used to determine the most important wavelength region for the prediction of these analytes. Fourier transform infrared spectroscopy did not yield any suitable models for the analytes investigated. Although the NIRS models developed were not capable of exactly quantifying phenolic or antioxidant content, infrared spectroscopy appears useful for rapidly discriminating between samples containing high and low levels of phenolics or antioxidant compounds. With further refinement, this technique could potentially be applied for the quality assurance of phenolic content or antioxidant capacity in faba bean seeds.
Microbially induced corrosion issues in the underground buried crude oil and natural gas bearing pipelines: A review
Authors: Bairi Lipika Rani , Bhuyan Pallabi , Ghosh Anirban , Narang Mandeep , Mandal Sumantra 
Published: August 2023

AbstractMicrobially induced corrosion (MIC) damage in crude oil and natural gas‐bearing pipelines is a severe threat for the petroleum industries. This is primarily due to the presence of hydrocarbons, which serve as nutrients for microorganisms. The comfortable growth of these microorganisms in hydrocarbon systems ultimately results in enhanced degradation of the pipelines. Hence, to understand the effect of microorganisms on the degradation of petroleum hydrocarbons and its subsequent influence on the corrosion of pipeline materials, a detailed review of the literature has been carried out. This review is primarily focused on the principal factors and the major microorganisms responsible for biofilm development. Furthermore, the role of these microorganisms in causing MIC in underground buried petroleum‐bearing pipelines has been reviewed in detail. Most importantly, the techniques required for studying MIC have been thoroughly discussed. In addition, a few microbial cases associated with various petroleum industries have been reviewed to acquire a clear understanding about the real situation.
Contributory Factors to Caregiver Burden in Parkinson's Disease
Authors: Soares Gabriela M. , Bouça‐Machado Raquel , Abreu Daisy , Ferreira Joauqim J. 
Published: August 2023

ABSTRACTBackgroundAlthough there is growing recognition of the relevancy of informal caregivers there is scarce information on the contributory factors of caregiver burden in Parkinson's Disease (PD).ObjectiveTo identify the main associated factors to caregivers’ burden in people caring for a person with PD.MethodsWe analyzed the data set from a multinational online survey the Parkinson's real‐world impact assesSMent (PRISM) focusing on medication use, comorbidities, health‐related quality of life, relationship changes and the use of healthcare and supportive care resources by people with PD and their carers. Structured questionnaires including the Parkinson's disease quality of life questionnaire (PDQ‐39), non‐motor symptoms questionnaire (NMSQuest) and the Questionnaire for impulsive‐compulsive disorder in Parkinson's disease (QUIP) were applied. Caregiver burden was assessed by the Zarit Burden Interview (ZBI).ResultsIn a cohort of 245 dyads (patient and respective caregiver), caregivers reported a mild to moderate burden. Carers’ perception of PD impact in partnership, financial burden, hours of care, patient's age, hypersexuality and health‐related quality of life (HRQoL) were found to be significant contributory factors to caregiver burden. Taken together these variables explained 66.8% of the variance in the Interpretation of the ZBI total score.ConclusionsCaring for a person with PD entails substantial burden, particularly when the caregiver perceives greater changes in partnership dynamics, dedicates more time to caregiving tasks, has financial burden, and when the patient is older, reports worst HRQoL and has sexual compulsive urges.
Next‐generation sequencing through multi‐gene panel testing for the diagnosis of a Chinese patient with atypical Cockayne syndrome
Authors: Wang Xinyi , Li Yue , Zhao Anqi , Wang Yumeng , Cao Qiaoyu , Pan Chaolan , Li Ming 
Published: August 2023

AbstractBackgroundCockayne syndrome (CS, OMIM #133540, #216400) is a rare autosomal recessive disease involving multiple systems, typically characterized by microcephaly, premature aging, growth retardation, neurosensory abnormalities, and photosensitivity. The age of onset is related to the severity of the clinical phenotype, which may lead to fatal outcomes.MethodsWe report a 3‐year‐old girl who presented with photosensitivity, gait abnormalities, stunting, and microcephaly and showed atypical clinical classification due to mild clinical manifestations at an early onset age.ResultsNext‐generation sequencing reveals the frameshift mutation (c.394_398del, p.Leu132Asnfs*6) and a novel microdeletion of ERCC8 (exon4del, p.Arg92fs).ConclusionTherefore, it is still necessary to carry out next‐generation sequencing for CS patients with atypical clinical manifestations, which is essential for diagnosis and accurate genetic counseling.
Negative interpretation bias in females with bulimia nervosa
Authors: Burmester Victoria , Nicholls Dasha 
Published: August 2023

AbstractPersons with bulimia nervosa (BN) often experience psychosocial difficulties, in particular heightened sensitivity to social rejection and a negative bias toward their social environment. Conversely, social competence and close friendships are protective against mental ill health. The aims of this study were to evaluate the interpretation of ambiguous social scenarios in females with and without BN and to assess the relationship between interpretation biases and clinical characteristics. Females with BN (n = 35) and controls (n = 35) were recruited via social media. Participants completed the Eating Disorder Examination Questionnaire (EDE‐Q), Adult Rejection Sensitivity Questionnaire, the Depression Anxiety and Stress Scales (DASS), and finished sentence stems depicting ambiguous social scenarios. Completed sentence stems were rated as positive, neutral, or negative by blinded researchers. Females with BN made fewer positive and more negative interpretations of sentence stems than controls. The frequency of negative interpretations correlated positively with clinical symptoms on the EDE‐Q, A‐RSQ, and DASS. A negative interpretation bias was found in females with BN, which aligns with the finding shown by Cardi et al. that females with anorexia nervosa have a negative interpretation bias toward ambiguous social scenarios. This bias was not only associated with eating disorder psychopathology but also with depression, anxiety, and stress, highlighting a potential transdiagnostic role. Interventions that address psychosocial difficulties might prevent the onset, reduce symptoms, and improve prognosis.
Developing a broadband band‐pass filtering power amplifier with enhanced performance
Authors: Liang Kun , Li Jia‐lin , Twumasi Baidenger Agyekum , Mung Steve W. Y. 
Published: August 2023

AbstractThis study presents a broadband and high efficiency hybrid inverse continuous filtering power amplifier (FPA) based on a terminated coupling line structure. The proposed FPA uses coupling lines as the main transmission line, and loading different stubs can simultaneously achieve input matching and output matching bandpass filtering. The four generated transmission zeros achieve good filtering performance. After discussing the matching structure, a broadband filtering PA operating at 1.7–2.4 GHz was designed and optimized. The measurement and simulation results showed good consistency. The best performance of the proposed PA was achieved at 2.1 GHz, with a gain of 11.24 dB, a saturation output power of 41.9 dBm, and a drain efficiency of 70.5%. The rejection of low and high frequency bands exceeds 49 and 55 dBc. The filtering PA has advantages in broad bandwidth and good out‐of‐band suppression.
Improved gray‐white matter contrast using magnetization prepared fast imaging with steady‐state free precession (MP‐FISP) brain imaging at 0.55 T
Authors: Schäper Jessica , Bauman Grzegorz , Bieri Oliver 
Published: August 2023

AbstractPurposeTo improve the gray/white matter contrast of magnetization prepared rapid gradient echo (MP‐RAGE) MRI at 0.55 T by optimizing the acquisition and sequence kernel parameters.MethodsA segmented magnetization prepared rapid gradient echo prototype sequence was implemented with (MP‐RAGE*) and without (MP‐FISP*) radiofrequency spoiling. Optimized parameters were derived with the assistance of an extended phase graph signal simulation as a function of the relaxation times, the flip angle, the delay times, and the effective inversion time using segmentation. The resulting protocols were compared to the MP‐RAGE product sequence offered by the vendor in terms of signal‐to‐noise ratio (SNR) and contrast‐to‐noise ratio (CNR). A tissue segmentation reproducibility study was performed on three volunteers for the product MP‐RAGE and the MP‐FISP*.ResultsThe MP‐RAGE simulation reproduced the parameters already used in the product MP‐RAGE on the scanner. An average CNR improvement of 15% for the custom MP‐RAGE* over the product MP‐RAGE and additional 22% for the MP‐FISP* over the MP‐RAGE* were observed, which is in accordance with the simulation results. The total improvement, averaged over all volunteers and regions, was 41%. The reproducibility study did not yield a significant difference between MP‐RAGE and MP‐FISP*.ConclusionWe presented some easy‐to‐implement adjustments to the MP‐RAGE sequence at 0.55 T, which can lead to an overall average improvement of 41% in CNR.
Multi‐echo single‐shot spectroscopy combined with simultaneous 2D model fitting for fast and accurate measurement of metabolite‐specific concentrations and T2 relaxation times
Authors: Rizzo Rudy , Kreis Roland 
Published: August 2023

The purpose of the current study was to develop a novel single‐voxel MR spectroscopy acquisition scheme to simultaneously determine metabolite‐specific concentrations and transverse relaxation times within realistic clinical scan times. Partly truncated multi‐TE data are acquired as an echo train in a single acquisition (multi‐echo single‐shot [MESS]). A 2D multiparametric model fitting approach combines truncated, low‐resolved short TE data with fully sampled, highly resolved, longer TE data to yield concentration and T2 estimates for major brain metabolites simultaneously. Cramer–Rao lower bounds (CRLB) are used as a measure of performance. The novel scheme was compared with traditional multi‐echo multi‐shot methods. In silico, in vitro, and in vivo experiments support the findings. MESS schemes, requiring only 2 min 12 s for the acquisition of three echo times, provide valid concentration and relaxation estimates for multiple metabolites and outperform traditional methods for simultaneous determinations of metabolite‐specific T2s and concentrations, with improvements ranging from 5% to 30% for T2s and from 10% to 50% for concentrations. However, substantial unsuppressed residual water signals may hamper the method's reproducibility, as observed in an initial experiment setup that prioritizes short TEs with severely truncated acquisition for the benefit of signal‐to‐noise ratio (SNR). Nevertheless, CRLB have been confirmed to be well suited as design criteria, and within‐session repeatability approaches CRLB when residual water is removed in postprocessing by exploiting longer and less truncated data recordings. MESS MRS combined with 2D model fitting promises comparable accuracy, increased precision, or inversely shorter experimental times compared with traditional approaches. However, the optimal design must be investigated as a trade‐off between SNR, the truncation factor, and TE batch selections, all of which influence the robustness of estimations.
Implications of tumour heterogeneity on cancer evolution and therapy resistance: lessons from breast cancer
Authors: Thakur Shefali , Haider Syed , Natrajan Rachael 
Published: August 2023

AbstractTumour heterogeneity is pervasive amongst many cancers and leads to disease progression, and therapy resistance. In this review, using breast cancer as an exemplar, we focus on the recent advances in understanding the interplay between tumour cells and their microenvironment using single cell sequencing and digital spatial profiling technologies. Further, we discuss the utility of lineage tracing methodologies in pre‐clinical models of breast cancer, and how these are being used to unravel new therapeutic vulnerabilities and reveal biomarkers of breast cancer progression. © 2023 The Pathological Society of Great Britain and Ireland.
Shape memory effect and thermal conductivity of PLA/h‐BN composites
Authors: Ostrovskiy S. D. , Krotenko I. A. , Stepashkin A. A. , Zadorozhnyy M. Yu. , Kiselev D. A. , Ilina T. S. , Kolesnikov E. A. , Senatov F. S. 
Published: August 2023

AbstractThe use of polylactic acid‐based composites as self‐fixing materials is a promising technology for various applications due to the shape memory effect of polylactide. In this study, hexagonal boron nitride (h‐BN) was mixed with polylactic acid (PLA) with concentrations of 0, 0.5, 1, 2, 5, and 10 wt% for subsequent film casting. The morphology, mechanical and thermal characteristics of the composite were investigated. With a high percentage of filler content, a decrease in thermal conductivity was observed. The recovery strain decreased with increasing the filler content. The amount of h‐BN had a mixed effect on the recovery stress of the composite. Thus, PLA/h‐BN composites represent a new research idea to study the influence of a heat‐conducting filler on a shape memory effect in polylactic acid.Highlights
Correlation between crystallinity, thermal and shape memory properties was described
Decrease in thermal conductivity was observed with the large percentage of filling
The composite with 2 wt% of h‐BN exhibits optimal characteristics of SME
Recovery strain decreased with the rise of filler content
Amount of h‐BN had a non‐trivial effect on the recovery stress of composite