bluuebunny/crossref_metadata_2025_split huggingface dataset:
SOX2 predicts local spreading and promotes a cancer stem cell-like phenotype in oral squamous cell carcinoma
Authors: Sacco Alessandro , Battaglia Anna Martina , Santamaria Gianluca , Buffone Caterina , Barone Selene , Procopio Anna , Lavecchia Anna Maria , Aversa Ilenia , Cristofaro Maria Giulia , Giudice Amerigo , Biamonte Flavia 
Published: January 2023

Abstract
Background
Emerging evidence points toward the idea that oral squamous cell carcinoma (OSCC) invasiveness can be attributed to the existence of a small subpopulation of cancer stem cells (CSCs) in the bulk of the tumor. However, knowledge about the existence of CSCs in the OSCC close margins and their possible role in determining OSCC phenotypes is still far from being satisfactory.
Methods
We analyzed the expression of 13 CSCs marker genes in 72 primary tissue specimens (24 core tumors, 24 pathologically negative close margins, and 24 healthy distant margins), obtained from 24 OSCC patients upon surgical resection, by using Real-Time PCR. TGCA and GEO databases were used to confirm gene expression analyses. CAL27 and SCC15 OSCC cell lines were used to perform in vitro siRNA-mediated knockdown of SOX2 both in 2D and 3D cultures. The effects of SOX2 knockdown on OSCC cell lines were assessed by wound healing assays and 3D tumor spheroids formation assays. Cytofluorimetric apoptosis assays were used to test the effects of SOX2 knockdown on cisplatin sensitivity. Live imaging was used to monitor OSCC cells migration and spheroidogenesis.
Results
We found that BMI1, CD44, SOX2, OCT4, UBE2C, CXCR4 were significantly up-regulated, while IGF1-R, KLF4, ALDH1A1, CD133, FAM3Cappeared down-regulated in the tumor core vs healthy distant margin. No differences were observed for NANOG and RRM2. SOX2, CD44, and CXCR4 were upregulated also in the close margin vs healthy distant margin. The high expression of SOX2 both in the tumor core and in the close margin significantly correlated with tumor size and lymph node compromise. In vitro experiments further showed that SOX2knockdown i) promotes the mesenchymal-to-epithelial transition and smooths the invasiveness, ii) attenuates the 3D tumor sphere-forming capacity and stemness-associated traits and iii) partially increases the sensitivity to cisplatin treatment of CAL27 and SCC15 cell lines.
Conclusions
Overall, our study shows that the OSCC close margins can retain CSC-specific markers even in the setting of negative histopathological diagnosis. In particular, SOX2 may represent a promising marker useful to predict a more aggressive OSCC phenotype and a suitable target to prevent local invasiveness.
Morphological abnormalities of orbitoidal foraminifera (Orbitoides and Omphalocyclus) from the Maastrichtian of the Zagros Mountains in northern Iraq
Authors: Nuaimy Qahtan A.M. Al 
Published: January 2023

Abstract

Many Orbitoides and Omphalocyclus individuals that were collected in the areas of Sura Qalat and Mokaba (northeast Sulaimani, Iraq) exhibited a number of aberrant characteristics. They contain exceptional examples of the microboring activity that took place within the tests, an uncommon test exterior form, the blossoming of equatorial chamber development, and peculiar reproductive characteristics. These aspects provide evidence for the existence of orbitoidal foraminiferal fossilized remains throughout the Maastrichtian period, as well as for palaeoecological variables, the biological lifetime, and taxonomic classification. The fossilization of these individuals and their associations with other creatures, such as Maeandropolydora osmaneliensis Nielsen, Gormüş, and Curvichnus semorbis Nielsen, are what induce the activity known as microboring. Conical forms and other unusual exterior shapes are thought to be linked to palaeoenvironmental sediment logical features such as the type of substratum and the currents that existed in that environment. It is believed that the biological and genetic traits of orbitoidal taxa have a considerable impact on the formation of lateral and equatorial chambers, in addition to the distinctive reproductive abilities these chambers possess. In the case of Orbitoides apiculatus Schlumberger, one of the most notable features is thought to be the cyclical flowering that occurs in the equatorial chambers.
Assessment of diclofenac in three selected rivers of Western Himalayan Region
Authors: Sharma Dheeraj , Choudhary Poonam , Gandotra Roopma 
Published: January 2023

AbstractThe present study reports the concentration of Non-steroidal anti- inflammatory drug (NSAID), Diclofenac in three main rivers namely Sarsa, Sutluj and Ravi of Western-Himalayan region. Diclofenac was analyzed from water samples using UPLC-S/MS system. The concentration ranged between 0- 2.05ng/l, the maximum concentration was observed in Badhi (S1) due to presence of number of pharmaceutical industries. The concentrations of diclofenac reported in the current study were comparatively very low as observed in other studies worldwide. This is the first report on the occurrence of diclofenac in the rivers of Western Himalayan region.
Spontaneous Bilateral Internal Carotid Arteries Dissection with Acute Stroke - A Case for Stent Implantation and Antithrombotic Therapy and literature review
Authors: li hong-yan , Wang Long-bing , Cai Zhi-you , Li Jing-lun 
Published: January 2023

Abstract
Background
Internal Carotid Arteries Dissection (ICAD) is a rare but important cause of severe acute ischemic stroke. Patients with ICAD are mostly treated with the antithrombotic regimen (anticoagulation, signal antiplatelet and dual antiplatelet), or endovascular treatment. The recurrence rate of carotid artery dissection stroke is relatively low. Here, we present a case of middle-aged male patient with acute ischemic strokes was caused by spontaneous bilateral ICAD. Stent placement in combine with antiplatelet therapy is a beneficial treatment method for such patient, as a preferred option.
Case presentation:
We present a case of a 54 -year-old male with no history of arteriopathy who presented with left limber weakness and slurred speech. Cerebral magnetic resonance imaging (MRI) showed acute large area cerebral infarcts in the right hemisphere. Digital subtraction angiography (DSA) demonstrated that the right internal carotid artery (ICA) occlusion at the cervical portion. He was found to have dissections in the right intracranial carotid arteries. He was treated with dual antiplatelet therapy and right carotid artery stent implantation. However, the patient still developed new neurological deficits, after stenting and balloon dilatation in the right carotid artery and postoperative antiplatelet therapy. MRI showed that acute left cerebral hemisphere infarction. Cervical computed tomography angiography and DSA revealed status of right carotid artery stent implantation and left ICA occlusion. Similarly, stent was performed in his left carotid artery, and dual antiplatelet therapy still continued. He was seen in the clinic at 6 months with follow-up imaging revealing improvement in the dissections.
Conclusion
The preferred option for patients with ICAD is to receive medication. If the ischemic event still occurs, invasive treatment should be carried out. However, ischemic stroke events were treated effectively with stenting and postoperative antiplatelet therapy.
Reconciliation of Thermodynamic and Mechanical Pressures and Development of a Frequency-Based Formula for Speed of Sound in Gases
Authors: Ogretim Egemen Ol 
Published: January 2023

Abstract
Discrepancy of the thermodynamic and mechanical pressures is a problem at the heart of the current theory of fluid mechanics. The fluid property that leads to this situation is the bulk viscosity, whose effects are zero for incompressible cases and are negligible for most other applications. Therefore, this discrepancy is conventionally ignored in phenomena other than acoustics and shock related ones. However, the flaw in the theory still persists since the late 19th century. In the present study, to improve the existing theory and to come up with a consistent structure in terms of mechanical and thermodynamic pressures, a novel fluid element model is proposed. Unlike the current fluid model that assumes a continuum of fluid, the present model proposes fluid elements that are separated from each other by a thin energy field that manifests itself as the mechanical pressure. Also, unlike the current efforts in explaining bulk viscosity effects through atomic level dynamics, the present model proposes a mesoscale analysis where bulk viscosity is integrated into the fluid element as a damper. Considering all these new features, each fluid element in this new model contains energy in both the wave form and the particle form. Of these two, wave energy is the cause of the thermodynamic pressure. In this manuscript, first, justifications of the mentioned aspects of the new fluid model are given. Then, a speed of sound expression is derived based on the new model involving the bulk viscosity effects. Resultant expression is, then, used for comparison with the findings of previous studies. The proposed formula can also be used to calculate the bulk viscosity of gases at different acoustic frequencies in a way that is more direct than those currently in use.
The Effect of COVID-19 on the Menstrual Cycle of Female Medical Staff: A Cross-sectional Survey in China
Authors: Sun Wenxi , Xia Liping , Ji Caifang , Wei Qianqian , Zhang Jun , He Sidi , Wang Xuqin , Shen Xueqin , Zhang Xiaobin , Li Chuanwei 
Published: January 2023

Abstract
Objective
Coronavirus disease 2019 (COVID-19) can affect women 's health. This study aimed to investigate the association between Menopathy, COVID-19 pandemic-related anxiety, sleep dysfunction and other factors among female medical staff.
Methods
A cross-sectional study was conducted via administrating online questionnaires to female medical staff in China from February to May 2022. The study includes 160 women aged between 18–45 years old. The questionnaires contain sociodemographic characteristics, medical and reproductive history, lifestyle information of participants, Rating Scale for Clinical Manifestation of Menopathy (SCMM), Self-Rating Anxiety Scale (SAS), and Sleep Dysfunction Rating Scale (SDRS). Data were analyzed using chi-square, t-tests, and linear regression analysis.
Results
A total of 160 female medical staff were randomly selected in this research, 7 of whom scored less than 3 points, 85 of whom scored more than 2 points and less than 12 points, and 68 of whom scored more than 11 points on the total score of SCMM. The score of dizziness and tinnitus was significantly higher during than before the COVID-19 pandemic, and scores of its following clinical symptoms of Menopathy: hypaphrodisia, dim complexion, abnormal urination, languidness, dim menstruation, thin menstruation, dysmenorrhea, and empty or saggy lower abdomen (p < 0.05). However, the score of vaginal bleeding quantity was significantly higher before than during the COVID-19 pandemic (p < 0.05). The score of vaginal bleeding quantity of the female medical staff was significantly lower in the cabin hospitals than others, and the same is true for vaginal bleeding duration (all p < 0.05). Besides, the findings of the univariable and multivariable linear regression analysis revealed a link between consistent exercise, the underlying illness, the SDRS score, the SAS score, and the total score of SCMM (p < 0.05).
Conclusions
In this study, we found that menstruation in female health staff was affected by the COVID-19 epidemic, where regular exercise and good physical condition were protective factors, while anxiety and insomnia were risk factors for regular menstruation.
Low Respiratory Quotient Correlates with High Mortality in Patients Undergoing Mechanical Ventilation
Authors: Shinozaki Koichiro , Yu Pey-Jen , Zhou Qiuping , Cassiere Hugh A. , John Stanley , Rolston Daniel M. , Garg Nidhi , Li Timmy , Johnson Jennifer , Saeki Kota , Goto Taiki , Okuma Yu , Miyara Santiago J. , Hayashida Kei , Aoki Tomoaki , Wong Vanessa , Molmenti Ernesto P. , Lampe Joshua , Becker Lance 
Published: January 2023

Abstract
Background
Oxygen consumption (VO2), carbon dioxide generation (VCO2), and respiratory quotient (RQ), which is the ratio of VO2 to VCO2, are critical indicators of human metabolism. To seek a link between the patient’s metabolism and pathophysiology of critical illness, we investigated the correlation of these values with mortality in critical care patients.
Methods
This was a prospective, observational study conducted at a suburban, quaternary care teaching hospital. Age 18 years or older healthy volunteers and patients who underwent mechanical ventilation were enrolled. A high-fidelity automation device, which accuracy is equivalent to the gold standard Douglas Bag technique, was used to measure VO2, VCO2, and RQ at a wide range of fraction of inspired oxygen (FIO2).
Results
We included a total of 21 subjects including 8 post-cardiothoracic surgery patients, 7 intensive care patients, 3 patients from the emergency room, and 3 healthy volunteers. This study included 10 critical care patients, whose metabolic measurements were performed in the ER and ICU, and 6 died. VO2, VCO2, and RQ of survivors were 282 +/- 95 mL/min, 202 +/- 81 mL/min, and 0.70 +/- 0.10, and those of non-survivors were 240 +/- 87 mL/min, 140 +/- 66 mL/min, and 0.57 +/- 0.08 (p = 0.34, p = 0.10, and p < 0.01), respectively. The difference of RQ was statistically significant (p < 0.01) and it remained significant when the subjects with FIO2<0.5 were excluded (p < 0.05).
Conclusions
Low RQ correlated with high mortality, which may potentially indicate a decompensation of the oxygen metabolism in critically ill patients.
Atypical Anti-Glomerular Basement Membrane Nephritis Occurring After SARS-CoV-2 Infection: A Case Report
Authors: Bağdatoğlu Oktay , Karakan Mine Şebnem , Süngü Nuran 
Published: January 2023

Abstract
Background
Atypical anti-glomerular basement membrane (GBM) disease, seronegative anti-GBM disease, is a variant where serum anti-GBM antibodies can not be identified in circulation although GBM is stained by Ig-G on an immunofluorescence microscope. We present a 19-year-old male patient who was previously healthy and developed the atypical anti-GBM disease after a SARS-CoV-2 infection.
Case presentation
The patient, who started to complain of hematuria and hemoptysis on the 3rd day of the negativity of the SARS-CoV-2 infection test, was referred to the hospital on the 15th day. The SerumAnti-GBM antibody of the patient who did not need hemodialysis was negative. Tubulointerstitial nephritis (TIN) was diagnosed in the kidney biopsy of the patient, and corticosteroid therapy was given. However, when the patient's complaints of hematuria and hemoptysis continued, a rebiopsy was performed. A diagnosis of atypical anti-GBM was made after linear IgG staining was detected in the glomerular basement membrane in the pathology of the patient whose serum anti-GBM antibodies were negative. Cyclophosphamide was given to the patient who was under corticosteroid treatment.
Conclusion
Although the classical anti-GBM disease is known to occur after SARS-CoV-2 infection, according to our knowledge, this case is the first case of atypical anti-GBM disease developed after SARS-CoV-2 infection.
Knowledge and Practical Attitudes of Caregivers Regarding Covid-19 at Sikasso Hospital in Mali in 2022
Authors: Sidi Haidara Dadé Ben 
Published: January 2023

Abstract

Background: Since the introduction of the coronavirus in Mali in March 2020, Sikasso Hospital was designated as a management center. The aim of this study was to determine the level of knowledge and practical attitudes of health care providers regarding covid-19.
Method / Results: This was a qualitative cross-sectional study conducted from December 10 to 12, 2020. Our sample was non-randomized for convenience, with 42 caregivers. Direct observation, questionnaire interview techniques were used. Data were analyzed on SPSS 23.0 and Excel 2017 software.
The average age was 42.3 ± 8.1 years; 85.7% of respondents were male versus 14.3% female; This population consisted of 31% Physicians/Pharmacists, 47.6% Nurses and 21.4% support staff.
The majority (69%) had a level of education that allowed them to make appropriate judgments about a health problem. All agents were informed about COVID-19 through the media, the department, their families or friends. The screening rate was 2.56 ‰. The majority of patients were health workers either (16.5%). The change in the status of the hospital as a center for the management of the disease, caused "fear, stress, panic" among the caregivers.
Conclusion: This study allowed us to determine the level of WTP of caregivers in the face of this disease at the hospital level.
Overexcitability and its Impact on Psychosomatic Disorders and the Role of "Cognitive Emotion Regulation" as a Mediating Variable
Authors: Alsaffar Khoula Fahad 
Published: January 2023

Abstract
Background
The current investigation examines TPD's assumption that bright people are overexcitable. The study will also reveal the impact of overexcitability on the degree of psychosomatic disorders among individuals regardless of talent. It was to examine what was assumed by the theory concerning the "amplified neurotic singularity" for the overexcitability and the possibility of improving their ability to be affected by psychological and physical orally. The study will also examine the "indirect influence" of positive and negative Cognitive Emotion Regulation as two mediating variables that control the effect of overexcitability on psychosomatic diseases.
Methodology
The descriptive analytical approach was utilized to test the study's assumptions using the Overexcitability Questionnaire II (OEQII), Cognitive Emotion Regulation Questionnaire (CERQ), and Patient Health Questionnaire PHQ-15 somatization on a sample of 269 students (male and female) (204 female).
Results
The study found that talented and normal pupils differed mainly in intellectual overexcitability (p=.044, 2.021T=). Track analysis demonstrated that emotional overexcitability and imaginative arousal have "direct favorable impacts" on psychosomatic diseases. P= 0.007- Z=2.681. Z=4.929 - P<.001. "Direct positive impacts" are for sensory and intellectual forms, negative effect on positive emotional regulation, direct negative effect for imaginational overexcitability, and positive effect of emotional overexcitability on negative emotional regulation.
Conclusions
Positive and negative emotional regulation as mediators showed no indirect connections between overexcitability and psychosomatic diseases. Considering the results, the study proposes future research on the effect of normal overexcitability on other psychological diseases and characteristics like attachment, social competence, and flexibility.
Delayed Onset of Neonatal Compartment Syndrome Associated with Compound Fetal Presentation
Authors: Manini Nicholas , Unno Hayato 
Published: January 2023

Abstract
Neonatal compartment syndrome, although rare, has a classic presentation with sentinel skin findings and subsequent development of swelling, erythema, and tenderness of the affected extremity. Neonatal compartment syndrome requires prompt surgical intervention to preserve the affected limb and ensure its normal growth and development. Our patient was born at term via vaginal delivery complicated by a compound presentation involving the left upper extremity. No physical exam abnormalities were noted at birth, but she developed signs of neonatal compartment syndrome by 15 hours of life. She was surgically treated at 22 hours of life and recovered well. At one year of age, she has normal growth and function of the affected extremity. Our case adds to the growing literature associating neonatal compartment syndrome with a compound fetal presentation.
Associations between Healthcare Costs and Care Experiences Among Older Adults with and Without Cancer
Authors: Allaire Benjamin , Zabala Diana , Lines Lisa M. , Williams Courtney , Halpern Michael , Mollica Michelle 
Published: January 2023

Abstract
Purpose
Care coordination and patient-provider communication are important for older adults with cancer, as they likely have additional, non-cancer chronic conditions requiring consultation across multiple providers. Suboptimal care coordination and patient-provider communication can lead to costly and preventable adverse outcomes. This study examines Medicare expenditures associated with patient-reported care coordination and patient-provider communication among older adults with and without cancer.
Methods
We explore SEER-CAHPS® linked data for differences in health care expenditures by care coordination and patient-provider communication experiences for beneficiaries with and without cancer. The cancer cohort included beneficiaries with ten prevalent cancer types diagnosed 2011-2019 at least 6 months before completing a CAHPS survey. Medicare expenditures were abstracted from Medicare claims data. Care coordination and patient-provider communication composite scores (range 0-100, higher scores indicate better experiences) were patient-reported in the CAHPS® survey. We estimated expenditure differences per one-point change in composite scores for patients with and without cancer.
Results
Our analysis included 16,778 matched beneficiaries with and without a previously diagnosed cancer (N=33,556). Higher care coordination and patient-provider communication scores were associated with significant reductions in expenditures among beneficiaries with and without cancer in the six months prior to survey response, ranging from $83 (SE=$7) to $90 (SE = $6) per month. Six months post-survey, reductions in expenditures of ranging $88 (SE =$6) to $106 (SE = $8) were found.
Conclusion
We found lower Medicare expenditures associated with higher care coordination and patient-provider communication scores. As the number of survivors living longer both with and beyond their cancer grows, addressing their multifaceted care and improving outcomes will be critical.