bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive analysis reveals the therapeutic value of TRPV1 in cancers
Authors: zhang Xuchao , Li Yanan , Guo Xianfeng , Gong Han , Li Ji , Wang Zi , Gao Ziling 
Published: February 2023

Abstract
Background
Transient receptor potential vanilloid subtype 1 (TRPV1) plays a pivotal role in neurons and is closely related to pain transduction. However, the role of TRPV1 in pan-cancer remains unclear.
Methods
Data visualization was performed using TCGA, GETx, GEPIA2, TIMER, TISIDB, DiseaseMeth, GeneMANIA, GSEA, and Depmap Portal database and R language.
Results
We explored the pan-cancer expression patterns and prognostic value of TRPV1 across multiple databases and found that TRPV1 served as a tumor suppressor in most cancers. In particular, genetic alteration and DNA methylation analysis across 33 tumors revealed that gene deletion and DNA hypermethylation may contribute to its downregulation. We further constructed a transcription factors-TRPV1 regulatory network and revealed a series of key upstream transcription factors of TRPV1 in certain cancers. Additionally, we studied the relationship between TRPV1 levels and the tumor microenvironment, immune cells, immune checkpoints, and its sensitivity to small-molecule inhibitors.
Conclusions
Our study curated both the genetic and epigenetic status of TRPV1 and its regulatory networks in cancers and highlighted that TRPV1 could serve as a prognostic biomarker and is correlated with immunotherapy and chemotherapy.
Purification and characterization of cysteine protease of Sarcocystis fusiformis from infected Egyptian water buffaloes
Authors: Barakat Amal Z. , Abdel-Aty Azza M. , Ibrahim Marwa K. , Salah Hala A. , Hegazy Usama M. , Azouz Rasha A.M. , Bassuiny Roqaya I. , Shaapan Raafat M. , Mohamed Saleh A. 
Published: February 2023

Abstract
Sarcocystis spp. infects water buffaloes (Bubalus bubalis) causing sarcocystosis. In the present study, Sarcocystis fusiformis was recognized in Egyptian water buffaloes based on histological observation and molecular analysis of internal transcribed spacer 1( ITS1), 18S ribosomal RNA (18S rRNA) and cytochrome c oxidase subunit I (COX-1) gene fragments. Chemotherapy and vaccines against Sarcocystis spp. could potentially target proteases because they may play a crucial role in the infection. Cysteine proteases are multifunctional enzymes involved in vital metabolic processes. However, the involvement of proteases in S. fusiform infection has not yet been characterized. Here, the purification and study on some biochemical properties of protease isolated from cysts of S. fusiform were carried out. Protease with a molecular weight of 100 kDa was purified. The purified protease exhibited maximum activity at pH 6 and a temperature of 50°C. The Michaelis-Menten constant (Km), the maximum velocity (Vmax) and the turnover number (Kcat) were determined. The complete inhibition effect of cysteine inhibitors indicated that the purified enzyme is cysteine protease. The results suggested that S. fusiform proteolytic enzyme may be necessary for parasite survival in the water buffaloes by digesting host tissues. Therefore, cysteine protease could be a suitable target for vaccinations.
A Robust Lateral Shift Free (LSF) Electrothermal Micromirror With Flexible Multi-Morph Beams
Authors: Xie Huikai , Yang Hengzhang , Ren Anrun , Ding Yingtao , Xiao Lei , Pan Teng , Yan Yangyang , Jiao Wenlong 
Published: February 2023

Abstract
Electrothermal bimorph-based scanning micromirrors typically employ widely-used silicon dioxide (SiO2) as the electrical and thermal isolation material. However, due to the brittle nature of SiO2, such micromirrors may not be able to even survive a slight collision, which greatly limits their application range. To improve the robustness of electrothermal micromirrors, a polymer material is incorporated to partially replace SiO2 as the electrical and thermal isolation material as well as the anchor material. In particular, photosensitive polyimide (PSPI) is used to simplify the fabrication process. In this work, PSPI-based electrothermal micromirrors have been designed, fabricated and tested. The PSPI-type micromirrors achieved a maximum optical scan angle of ± 19.6 ° and a maximum vertical displacement of 370 µm both at only 4 Vdc. With a mirror aperture size of 1mm × 1 mm, the PSPI-type micromirrors withstood over 200 g accelerations from either vertical or lateral directions in the impact experiment. In the drop test, the PSPI-type micromirrors survived falls to a hard floor at heights up to 21 cm. In the standard frequency sweeping vibration test, the PSPI-type micromirrors withstood 21 g and 29 g acceleration in the vertical and lateral vibration, respectively. In all these experimental tests, the PSPI-type micromirrors demonstrated at least 4 times better robustness compared to the SiO2-type micromirrors fabricated in the same batch.
Hyperthermia inhibited cellular function and induced immunogenic cell death of renal cell carcinoma
Authors: Huaqi Yin , Bingqi Dong , Yanhui Zhao , Yongkang Ma , Shiming Zhao , Zhenghui Sun , Zheng Du , Jiangshan Peng , Tiejun Yang 
Published: February 2023

Abstract
Background: As a novel strategy, hyperthermia has been widely applied to cancer treatment recent years for its multiple anti-tumor effects. Especially, the potential influences of hyperthermia on tumor immune microenvironment may contribute to improve the efficacy of immunotherapy. However, the role of hyperthermia on renal cell carcinoma (RCC) is not well understood until now.
Methods: In present study, we primarily evaluated the effects of hyperthermia on cellular function via cellular proliferation, migration assay, invasion and apoptosis assays. In addition, the influence of hyperthermia on immunogenicity of RCC cells were analyzed using flow cytometry analysis, enzyme-linked immunosorbent assays and IF staining.
Results: our results showed that hyperthermia could significantly inhibit the proliferation, migration and invasion of RCC cells, and promote cell apoptosis. In addition, hyperthermia was verified to improve the immunogenicity of RCC cells via inducing immunogenic cell death.
Conclusion: Our data demonstrated that hyperthermia was expected to be a promising therapeutic strategy for RCC.
Neonatal hypoglycemia: What can we improve?
Authors: Delgado Carolina Solé , Ros Itziar Marsinyach , Luna Manuel Sánchez 
Published: February 2023

Abstract
Background: Neonatal hypoglycemia is a frequent problem with potential neurological adverse effects, and it involves a significant number of admissions to neonatal units with the consequent mother-child separation, difficulty in establishing breastfeeding, and increased hospital expenditure.
Although any newborn can suffer hypoglycemia, certain patients are at higher risk, such as late preterm infants, those born with low birth weight or high birth weight for their gestational age, or children of mothers with diabetic pathology. Strategies to detect and prevent hypoglycemia in this subgroup of patients is a common practice in neonatology units.
This study aims to analyze the impact of this situation in our center, in order to identify , areas for improvement.
Material and methods: we performed a retrospective review of medical charts of newborns, at term or near term, admitted for hypoglycemia in a tertiary hospitalization center during 2019 and 2020.
Results: 232 patients were admitted for hypoglycemia during this period, accounting for 11.5% of total admissions. Of these, 185 (79%) had known risk factors for hypoglycaemia. The median gestational age was 37 (interquartile range (IQR) 36-38) and weight 2450 g (IQR 2255 - 2935 g). The most frequent risk factor was late prematurity (30.8%), followed by low birht weight (26%). Ninety percent had a pathological first blood glucose level, the value of which correlated inversely with the time of fluid therapy and, also, with the age at admission (p=0.02. Pearson correlation (PC) -0.172). The median cronological age at admission was 6 (RIQ 4-10). 16.75% of the patients (31/185) had not taken early enteral feedings (neither formula bottle nor breastfeeding) before the first glycemic control. A total of 42.7% were exclusively breastfed (BF), 31.3% fed with adapted formula (AF), and in 9.5% of the patients it could not be determined whether they had received or not an early feeding. The mean of the first glycemic value was significantly lower in those who did not take an early feed (mean 28.4, SD 1.7) than those who did (mean 35.2 SD 0.88) and was lower in those who were admitted earlier (mean 5.9 hours SD 0.44 vs. 8 hours SD 0.56).
Conclusion: Admissions for neonatal hypoglycemia are frequent. Early intake was associated with a higher first glycemic control. This measure was only fulfilled within some patients in our center, so there is an opportunity for improvement.
Trial registration: Retrospectively registered
Alkyl-Parity Controlled Switching of Polar/Antipolar Organic Semiconductors
Authors: Inoue Satoru , Higashino Toshiki , Nikaido Kiyoshi , Miyata Ryo , Matsuoka Satoshi , Tanaka Mutsuo , Tsuzuki Seiji , Horiuchi Sachio , Kondo Ryusuke , Sagayama Ryoko , Kumai Reiji , Hasegawa Tatsuo 
Published: February 2023

Abstract
The odd-even effects are renowned as a mysterious phenomenon in broad fields of science but have never been established as an effective approach for materials engineering. We demonstrate that the parity of alkyl carbon number n can cause alternating emergence of polar/antipolar organic semiconductor crystals. This is achieved by the development of a series of polar rod-like molecules, composed of a linkage between extended π-core (head) and alkyl chains (tail), exhibiting both high layered crystallinity and well-balanced end-to-end affinity. The molecules are unidirectionally aligned to form two-dimensional array, and the eventual polar monomolecular layers present two distinct types of interlayer stacking depending on the parity of n: alternating head-to-head and tail-to-tail (antipolar) alignment in odd-n crystals, and uniform head-to-tail (polar) alignment in even-n crystals. The latter allows to obtain polar semiconductor films that considerably improve interfacial carrier transport characteristics. The findings are key for creating polarity-controlled optoelectronic materials and devices.
1,3,4-Oxadiazole derivatives as potential antifungal agents: Synthesis, characterization, Ct-DNA binding, molecular docking, and TD-DFT studies
Authors: Mehandi Rabiya , Sultana Razia , Ahmedi Saiema , Fatima Aysha , Islam Khursheed ul , Rana Manish , Manzoor Nikhat , Javed Saleem , Haque Mohammad Mahfuzul , Iqbal Jawed , uddin Rahis , Nishat Nahid 
Published: February 2023

Abstract
1,3,4-Oxadiazole-based heterocyclic analogs (3a–3m) were synthesized via cyclization of Schiff bases with substituted aldehydes in the presence of bromine and acetic acid. The structural clarification of synthesized molecules was carried out with various spectroscopic techniques such as FT-IR, 1H and 13C-NMR, UV–visible spectroscopy, mass spectrometry (LCMS). The TD-DFT studies were also confirmed the structure of drug molecules. In vitro antifungal activity was performed against C. Albicans, C. glabrata and C. tropicalis and analogs 3g, 3i, and 3m showed potent MIC at 200 µg/ml and excellent ZOI measurements of 17-21 nm. The cell viability on human hepatoma cells (Huh7) for lead molecules 3g, 3i, and 3m was found to be 99.5%, 92.3%, and 86.9% at 20 μM, 10 μM, and 20 μM respectively. The antioxidant activity of the lead molecules 3g, 3i, and 3m were estimated and exhibited great IC50 values of 0.104 ± 0.021, 0.145 ± 0.05, and 0.165 ± 0.018 μg/mL with DPPH and 0.107 ± 0.04, 0.191 ± 0.12, and 0.106 ± 0.08 with H2O2 respectively. The DNA binding interaction mode for the lead molecules was also carried out with Ct-DNA using the absorption, emission, CV, CD, and Time resolve fluorescence techniques. The results showed good binding constant (Kb) values 9.1×105, 9.94×105, and 9.32×105 M−1 for 3g, 3i, and 3m respectively. The results were further validated by In-silico molecular docking and pharmacokinetics properties of lead drug molecules were also studied with PDB ID: 1BNA and 5FSA to explore the best hits.
Exploring the volatility spillover dynamics among Non-fungible Token Coins and Bitcoin
Authors: Kumar Anoop S , Padakandla Steven Raj , Suvvari Anandarao 
Published: February 2023

Abstract
We explore the volatility spillover dynamics between Non-fungible token coins ( NFTC) and Bitcoin. Towards this, we employ DCC GARCH models on daily returns of Bitcoin along with three NFTCs, namely Decentral land, Theta and Eijin coin from 18-01-2018 to 03-12-2021. Our results show significant return and volatility spillover between NFTCs and Bitcoin. We find that the NFTC market is significantly affected by fluctuations in the Bitcoin market. Based on the results, we advise against the inclusion of NFTCs in a cryptocurrency portfolio
JEL Codes: G110, G150, C580
An experimental study on the conversion of CaO to CaSO4 during diesel particulate filter regeneration
Authors: Tanaka Kotaro , Sakai Yasuyuki , Ishii Yudai , Yokobayashi Yusuke , Sakaida Satoshi , Konno Mitsuru 
Published: February 2023

Abstract
The deposition of ash on a diesel particulate filter (DPF) results in an increase in pressure drop across aftertreatment system, which leads to reduced capacity for soot and an increase in frequency of regeneration to eliminate the soot. To estimate the amount of ash and design a reduction method for the accumulated ash on the DPF, a deeper understanding of the ash generation mechanism is required. Previous studies have found that ash sampled at exhaust manifolds mainly comprises typical metal oxides, whereas ash accumulated on DPFs primarily comprises typical metal sulfates. However, the conversion mechanism from metal oxides to metal sulfates is not well understood. Herein, the formation mechanism of calcium sulfate (CaSO4), which is the main component of ash on DPFs, was investigated using a flow reactor. Calcium oxide (CaO), which is the main component of ash sampled at exhaust manifolds, was made to react with SO2, SO3, O2, and H2O, when passed through a diesel oxidation catalyst. Quantitative analysis of the ash components in the products was performed using high-performance liquid chromatography. The concentrations of SO2, SO3, and H2O during the reaction were analyzed by Fourier-transform infrared spectroscopy. Based on the experimental results, it was found that CaSO4 mainly formed from the reaction of CaO with SO2 and SO3; whereas, H2O inhibited CaSO4 formation owing to the change in physicochemical properties of CaO.
Intravenous sildenafil for treatment of early and late pulmonary hypertension in preterm infants
Authors: Schroeder Lukas , Monno Paulina , Strizek Brigitte , Dresbach Till , Mueller Andreas , Kipfmueller Florian 
Published: February 2023

Abstract
Objectives

Data is lacking on the effect of continuous intravenous sildenafil treatment in preterm infants with early- and late-pulmonary hypertension (PH), especially in extremely low birth weight (ELBW) infants.

Patients and Methods

Preterm infants (<37 weeks of gestational age) with intravenous sildenafil treatment and diagnosis of PH between 01/12 and 12/21 were retrospectively screened for analysis. The primary clinical endpoint was defined as response to sildenafil according to the improvement of the oxygenation index (OI), the saturation oxygenation pressure index (SOPI) and PaO2/FiO2-ratio. Early-PH was defined as diagnosis <28 day of life (DOL), late PH ≥28 DOL.

Main Results

60 infants were finally included, with 47% ELBW infants. Early-PH was diagnosed in 97% and the primary endpoint was reached in 58%. The likelihood to die during in-hospital treatment was more than three times higher (72 vs 20%, p<0.001) in infants without response to sildenafil. The echocardiographic severity of PH and right-ventricular dysfunction (RVD) decreased significantly from baseline to 24 h (p=0.038, and p=0.009, respectively).

Conclusion

Sildenafil treatment leads to significant improvement of the oxygenation impairment in 58% of the preterm infants, with similar response rates in ELBW infants. Intravenous sildenafil treatment is associated with a significant decrease of the PH-severity and RVD.
Supervised Fuzzy control strategy for Mean Arterial Pressure regulation using cascade scheme controller: A Continuum Approach of hypertension with diabetics
Authors: A Alavudeen Basha , Ali Azath Mubarak , Tayfour Omer Elsier , Changalasetty Suresh Babu , Shuja Muhammed , P Parthasarathy 
Published: February 2023

Abstract
This paper presents a cascade methodology towards the control of blood pressure for perioperative patients with a diabetic continuum. Recent clinical research confirms that blood pressure and diabetes have possible overlap on their substantial and may occur together as per their disease etiology. Postoperative stress and hasty diabetic variation will make pressure distinctions, leading to cardiovascular catastrophe, hypo, hyperinsulinemia, and osmotic diuresis. To avoid anomalies, blood pressure and diabetes need to monitor simultaneously. Based on the distinctions, blood pressure has to control the optimal drug delivery system. This proposal adopts a cascade control modelling and control scheme strategy adopted with two different parameters in control. The control loop is overseen and eliminates the disturbances generated by diabetic variations, and the primary control loop supervises and controls drug delivery. In particular, the difference of the control is to produce automated correction of the drug SNP (sodium nitroprusside) infusion to maintain the Mean Arterial pressure (MAP) as prescribed. The proposed advisory process is based on FLC (Fuzzy logic) controller to optimize appropriate and optimal drug delivery. The cascade control studies' simulations demonstrated, that the proposed approach provides proper estimating drug delivery and regulates hypertension as per the recommended levels.
Another scanning test of trend change in regression coefficients applied to monthly temperature on global land and sea surfaces
Authors: Jiang Jianmin 
Published: February 2023

Abstract
Two algorithms has been proposed in this paper. One is another scanning t-test of trend change-points in regression slope-coefficients in two phases, along with a coherency analysis of changes between two time series. It is different from the previously published scanning Fmax test of trend changes in two-phase regressions. The second is a fuzzy weighted moving average (FWMA). Then the algorithms were applied to two series of monthly temperature over global land and ocean surfaces for 1850–2018. The applied results show that significant changes in segment trends appeared into two gradations on inter-decadal and intra-decadal scales. All subsample regression models were found to fit well with that data. Global warming got started in April 1976 with a good coherency of warming trends between land and sea. The global warming ‘hiatus’ mainly occurred in the sea cooling from November 2001 to April 2008, but not evinced over land. The ‘land/sea warming contrast’ was detected only in their anomaly series. It disappeared in their standardized differences. We refer to the anomalies in distribution N(0,s) as ‘perceptual’ indicators, while refer to the standardized differences in distribution N(0,1) as ‘net’ indexes.
The impact of pre-transplant anti-HLA antibodies in transplants from HLA-identical sibling donors: a multicenter study
Authors: Wu Xiaojin , Wei Xiya , Chang Ying-Jun , Zhu Xiaoyu , Hu Xiaoxia , Guo Rong , Zhang Yanming , Ma Xiao , Han Yue , Wang Ying , Qiu Huiying , Wu Depei 
Published: February 2023

Abstract
Few studies have performed comparative analysis of the outcome of hematopoietic stem cell transplantation from HLA-identical sibling donors (ISD-HSCT) in patients with or without anti-HLA Abs. In this study we retrospectively collected data from a multicenter study to analyze the distribution and impact of the pre-existing anti-HLA Abs in ISD-HSCT. Among 402 recipients, 111 were tested with anti-HLA Abs. Gender, time from diagnosis to transplantation and distribution of primary disease might be risk factors for the occurrence of anti-HLA Abs. We found that patients with anti-HLA Abs had delayed neutrophil engraftment and were more vulnerable to experience Cytomegalovirus (CMV) reactivation. The presence of anti-HLA Abs was proved to be an independent risk factor for neutrophil engraftment (HR 1.42 95%CI 1.13-1.80, P=0.003) and CMV reactivation (HR 2.03 95%CI 1.19-3.46, P=0.009). We found that anti-HLA Abs have a negative impact on the prognosis in the early period after transplantation from sibling donors and anti-HLA Abs was also an independent risk factor for the overall survival (OS) at 180 days (HR 2.32, 95%CI 1.03-5.27, P=0.042) among female recipients. In conclusion, anti-HLA Abs have a negative impact on the prognosis early after ISD-HSCT.
Molecular Epidemiology and Characteristics of Respiratory Syncytial Virus in Guangzhou, China
Authors: Umar Sajid , Yang Rongyuan , Wang Xinye , Liu Yuntao , Ke Peifeng , Qin Sheng 
Published: February 2023

AbstractBackground Human respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory tract infection and hospitalization, especially in children. Highly mutagenic nature and antigenic diversity enable the RSV to successfully survive in human population. Regular monitoring of the circulating strains of RSV in different parts of China could assist in the development of more effective vaccines and preventive measures. We performed a molecular epidemiological study during 2017–2021 to investigate the prevalence and genetic characteristics of RSV in China. Methods A total of 6499 nasopharyngeal (NP) swabs were collected from patients suffering from respiratory infections at Department of Pediatrics, Guangdong Provincial Hospital of Traditional Chinese Medicine, Yuexiu District, Guangzhou, Guangdong, China. Results Overall detection rate for respiratory viruses was 16.12% (1048/6499). A total of 405 specimens (6.20%, 405/6499) were found positive for RSV. Seasonal distribution of RSV and other respiratory viruses was variable and highest incidence were recorded in winter. Based on sequencing of hypervariable region of G gene, 93 RSV sequences were sub-grouped into RSV-A (56, 60.2%) and RSV-B (37, 39.8%). There was no coinfection of RSV-A and RSV-B in tested samples. Phylogenetic analysis revealed that RSV-A and RSV-B strains belonged to ON1 and BA9 genotype respectively, indicating predominance of these genotypes in Guangzhou. Deduced amino acid sequence analysis determined several substitutions which may likely change antigenicity and pathogenicity of RSV. Multiple glycosylation sites were observed in the second hypervariable region of the G protein, demonstrating high selection pressure on these genotypes. Conclusion This study illustrated the useful information about epidemiology, genetic characteristics and circulating genotypes of RSV in Guangzhou China which may facilitate policy makers, clinicians, and researchers for a better understanding of the evolution of RSV within and outside of China. Due to emergence of several lineages of ON1 and BA9 genotypes, it is quite possible that new lineages could emerge in near future due to highly mutagenic nature of RSV. Continuous and long-term surveillance programs coupled with clinical data must be initiated in Guangzhou to better understand the pattern of seasonal distribution of circulating genotypes of RSV and find any association between emerging genotypes and disease severity.