bluuebunny/crossref_metadata_2025_split huggingface dataset:
Population genetics and pedigree geography of Trionychia japonica in the four mountains of Henan Province and the Taihang Mountains
Authors: Zhu Hui , Chen Zhichao 
Published: April 2023

Abstract

Objective
Human activities and climate change have changed the living environment of species, accelerated the rate of species extinction, and global biodiversity is facing a huge threat. The objective of this study is to determine the resource protection strategy of freshwater planarian by systematically exploring the population genetics and pedigree geography of the planarian population in the four mountain systems of Henan Province and Taihang Mountains.

Methods
Taking the Japanese planarian in the four mountain systems of Henan Province and Taihang Mountains as an example, DNA was extracted with the help of mitochondrial cytochrome c oxidase subunit I gene (COI), mitochondrial cytochrome b gene (Cytb), and nuclear gene internal transcribed spacer-1 (ITS-1), amplified by polymerase chain reaction and sequenced, and genetic diversity, genetic structure, and pedigree geographical pattern were analyzed by single-gene analysis and polygene joint analysis.

Results
The corresponding length of Cytb, COI, and nuclear gene ITS-1 sequences in the four mountains of Henan Province is 308, 805, and 632 bp, respectively, and the nucleotide diversity and haplotype diversity are 0.00212 and 0.975, respectively. The gene length of ITS-1 and Cytb in Taihang Mountains is 668 and 315 bp, respectively, with a total length of 984 bp. There are 10 shared haplotypes and 36 unique haplotypes. The values of all nucleic acid diversity and haplotype diversity were 0.00156 and 0.965, respectively, and the genetic variation coefficients between populations and groups were 20.28% and 16.40%, respectively (P < 0.05). Different haplotypes of the four mountain systems in Henan Province and Taihang Mountains are scattered in different geographic populations, and there is no correlation between them and their geographic locations, which is consistent with the results of phylogeny.

Conclusion
The genetic diversity of Trionychia japonica population in the four mountain systems of Henan Province and Taihang Mountains shows high haploid diversity and low nucleotide diversity, and phylogenetic analysis has not found obvious pedigree geographical pattern.
From demonstrative to filler:estein Amazonian Spanish and beyond
Authors: Vallejos-Yopán Rosa 
Published: April 2023

AbstractIn Amazonian Spanish,estehas two main functions: demonstrative and filler. Filler-este, which originally evolved from demonstrative-este, serves to deal with word-formulation delays during spontaneous speech production. Analyses of conversations reveal thatesteprimarily functions as a filler: 70% of the tokens ofesteare either fillers serving as placeholders, which replace lexical items in specific syntactic slots, or fillers serving as hesitators, which are non-referential and distributionally free. Further, phonetic analyses show that demonstrative-esteand filler-esteexhibit different phonetic shapes. Demonstrative-estepatterns with disyllabic words with penultimate stress – the first vowel is longer than the second vowel. Filler-esteshows the opposite configuration – the second vowel is significantly longer than the first vowel. The evolution ofestefrom demonstrative to filler may have been facilitated by two conspiring forces: its use as cataphor whose referent comes later in the discourse, and its use for recognitional purposes, in which establishing a referent relies on shared knowledge. This use is often accompanied by signs of hesitation. The diachronic proposal outlined here can account for the emergence of the filler-estein several varieties of Spanish spoken in Latin America. Overall, this study contributes to our understanding of the syntax of real-time interaction.
The function of lncRNA EMX2OS/miR-653-5p and its regulatory mechanism in lung adenocarcinoma
Authors: Ma Lina , Zhang Lu , Li Lin , Zhao Li 
Published: April 2023

Abstract
This study aimed to evaluate the significance of EMX2OS in lung adenocarcinoma (LUAD) prognosis and development and its potential molecular mechanism. Paired tissue samples were collected from 117 LUAD patients. The EMX2OS expression level was detected by PCR and correlated with patients’ clinicopathological features by a series of statistical analyses. The function of EMX2OS in cell proliferation and metastasis was evaluated by CCK8 and Transwell assay. In mechanism, the interaction between EMX2OS and miR-653-5p was assessed by the dual-luciferase reporter assay, and the regulatory effect of miR-653-5p on EMX2OS tumor suppressor role was also estimated. Significant downregulation of EMX2OS and its negative correlation with miR-653-5p was observed in LUAD tissues. A significant relationship was revealed in EMX2OS with TNM stage, lymph node metastasis, and differentiation of LUAD patients, and associated with the poor prognosis of patients. EMX2OS suppressed the proliferation and metastasis of LUAD cells and negatively regulated the expression of miR-653-5p. The overexpression of miR-653-5p could reverse the inhibitory effect of EMX2OS on LUAD cells. In conclusion, EMX2OS served as a biomarker in LUAD that indicated patients’ prognosis and regulated cellular processes via regulating miR-653-5p.
The 27th Scientific Conference of the Society on NeuroImmune Pharmacology: New Delhi, India, March 15–18, 2023
Authors: Chang Sulie L. , Seth Pankaj , Zhu Jun , Pendyala Gurudutt , Bidlack Jean M. , Kumar Santosh 
Published: April 2023

Abstract
The 27th Scientific Conference of the Society on Neuroimmune Pharmacology (SNIP) in New Delhi, India, on March 15–18, 2023 is a historic summit of experts from around the world. The four day conference provides insights into the latest and most advanced science in the intersecting areas of neuroscience, immunology, pharmacology, and its translational aspects, in particular, HIV and drug abuse. Abstracts are ordered in three major groups: (1) Symposium speakers (S1–S64), (2) Investigator Posters (I1–I18), and (3) Trainee Poster (T1–T28).
Responses of Cortical Neurons to Intracortical Microstimulation in Awake Primates
Authors: Yun Richy , Mishler Jonathan H. , Perlmutter Steve I. , Rao Rajesh P. N. , Fetz Eberhard E. 
Published: April 2023

AbstractIntracortical microstimulation (ICMS) is commonly used in many experimental and clinical paradigms; however, its effects on the activation of neurons are still not completely understood. To document the responses of cortical neurons in awake nonhuman primates to stimulation, we recorded single-unit activity while delivering single-pulse stimulation via Utah arrays implanted in primary motor cortex (M1) of three macaque monkeys. Stimuli between 5 and 50 μA delivered to single channels reliably evoked spikes in neurons recorded throughout the array with delays of up to 12 ms. ICMS pulses also induced a period of inhibition lasting up to 150 ms that typically followed the initial excitatory response. Higher current amplitudes led to a greater probability of evoking a spike and extended the duration of inhibition. The likelihood of evoking a spike in a neuron was dependent on the spontaneous firing rate as well as the delay between its most recent spike time and stimulus onset. Tonic repetitive stimulation between 2 and 20 Hz often modulated both the probability of evoking spikes and the duration of inhibition; high-frequency stimulation was more likely to change both responses. On a trial-by-trial basis, whether a stimulus evoked a spike did not affect the subsequent inhibitory response; however, their changes over time were often positively or negatively correlated. Our results document the complex dynamics of cortical neural responses to electrical stimulation that need to be considered when using ICMS for scientific and clinical applications.
HIF 1 ɑ – a promising target for the treatment of meningiomas
Authors: Butta Shristi , Gupta Manoj Kumar 
Published: April 2023

Background. Meningiomas are the most frequent tumors of the brain and spinal cord with a potency to recur in around one third of the cases and and invade surrounding tissue. Hypoxia driven factors like HIFs (Hypoxia inducible factors) are implicated in tumor cell growth and proliferation.
Aim. This study aims at determining the association of HIF 1 ɑ with different histopathological grades and types of meningiomas.
Methods. This prospective study was conducted on 35 patients. The patients presented with headache (65.71%), seizures (22.86%) and neurological deficits (11.43%). They underwent surgical excision and surgical tissue samples of these patients were histopathologically processed and microscopically graded and typed. Immunohistochemistry was performed using anti-HIF 1ɑ monoclonal antibody. The nuclear expression of HIF 1 ɑ was graded as <10%: negative, 11-50%: mild to moderate positive, >50%: strong positive.
Results. Of the 35 cases so examined 20% were recurrent; 74.29% were WHO grade I with meningothelial type (22.86%), being the commonest; 57.14 % revealed mild to moderate positivity for HIF 1ɑ, while strong positivity was noted in 28.57%. Significant association was found between WHO grade and HIF 1ɑ (p=0.0015) and between histopathological types and HIF 1ɑ (p=0.0433). Furthermore, HIF 1ɑ was also significantly associated with the recurrent cases (p=0.0172).
Conclusion. HIF 1ɑ appears to be a marker and a promising target for effective therapeutics in meningiomas
Bleeding and thromboembolic risk in patients under anticoagulant therapy receiving oral surgery: a systematic review
Authors: Moldovan Madalina A. , Filip Laura V. , Ciurea Mircea , Termure Dragos A. , Ostas Daniel , Rotar Horatiu , Faur Cosmin I. , Roman Rares C. 
Published: April 2023

Background and aims. There is an increasing number of patients with cardiovascular diseases who require anticoagulant treatment to address the underlying disease. Types of anticoagulants include vitamin K antagonists, such as warfarin and coumarin derivatives, and also newer oral anticoagulants, including rivaroxaban, apixaban, edoxaban, and dabigatran. The use of these anticoagulants may impact the condition of patients undergoing oral surgery. If the treatment is discontinued, the patient may be at risk of thrombosis. On the other hand, if the treatment is continued, the patient may experience a postoperative bleeding episode, placing them at risk of both thrombosis and bleeding.
Method. The present article systematically reviews two different therapeutic regimens and their influence on hemorrhagic and thromboembolic events. The review included research from three databases and four specialized journals. The regimens examined were continuous versus discontinuous anticoagulant treatment and continuous versus interruption and switch to bridging therapy.
Results. The most common surgical procedure examined in the review was tooth extraction, with a few studies also including soft tissue procedures. A total of seven eligible articles were identified, with five using the first treatment regimen of continuous versus discontinuous anticoagulant. These studies reported several cases of bleeding under continuous anticoagulant treatment during surgery. Two articles used the second treatment regimen of continuous versus interruption and switch to bridging therapy.
Conclusions. The results of both treatment categories (continuous versus discontinuous anticoagulant and continuous versus interruption and switch to bridging therapy) showed no significant differences in terms of bleeding events. However, the use of scores that assess the risk of thrombosis and bleeding can assist surgeons in anticipating the degree of ostoperative complications and making informed treatment decisions.
Karakteristik Fisiko-Kimia Dan Proses Produksi Pakan Apung Ikan Lele (Clarias sp.)
Authors: Sayuti Mohammad , Dewi Larasati Rama , Sofian Achmad 
Published: April 2023

Salah satu komoditas unggulan pada budidaya ikan air tawar adalah ikan lele. Proses budidaya ikan lele membutuhkan ketersediaan pakan. Kebutuhan pakan masih didominasi pakan komersil dengan harga yang tinggi. Pakan buatan menjadi alternatif pemenuhan kebutuhan pakan budidaya ikan lele. Penelitian ini bertujuan untuk mengetahui proses pembuatan dan kualitas yang terkandung pada pakan apung untuk ikan lele. Penelitian dilaksanakan dari 01 Maret 2021 sampai 30 Mei 2021 di KUB Timoer Mandiri, Jember, Jawa Timur. Observasi langsung dilakukan untuk mengetahui proses pembuatan pakan. Pengujian pakan apung dilakukan dengan uji proksimat dan uji fisik pakan. Pengujian proksimat pada bahan dan pakan yang diproduksi sesuai standar AOAC 2005. Pengujian fisik pakan meliputi pengujian warna, bau, lama mengapung, kecepatan tenggelam, daya apung dan pengujian stabilitas pakan yang dilakukan tanpa aerator. Proses produksi pakan apung ikan lele meliputi persiapan bahan, penepungan, formulasi pakan, penimbangan, pencampuran, pencetakan, pengeringan, pendinginan, pengepakan dan penyimpanan. Hasil pengujian proksimat bahan baku pakan sudah memenuhi untuk pembuatan pakan. Pakan apung ikan lele memiliki kandungan protein 31.17%, abu 6.86%, lemak 5.29%, serat 6.17%, air 10.35%, BETN 39.76%, gross energy 3.891,43 Kcal/kg pakan dan digestible energy 31.131.419 Kcal/kg pakan. Pakan apung memiliki warna coklat cerah, bau menyengat, lama mengapung 424 menit, kecepatan tenggelam 0.39 cm/detik, daya apung 100% dan pakan hancur di dasar pada menit 480. Pakan apung ikan lele yang dihasilkan memiliki kualitas baik fisik dan kimia yang menyerupai dengan pakan komersial.
Utilization of Remote Sensing Images for Compiling The Characteristics Map of Indramayu Beach, West Java
Authors: Adjisuci Arifah Fadhilah , Sewiko Roni , Mawardi Sonny , Anasri Anasri 
Published: April 2023

The beach has an important role in the development and economy of a region. Therefore, it is important to understand the condition and characteristics of the coast so that it can be managed and developed wisely. One way to understand the condition of the beach is through making a map of the characteristics of the beach. A coastal characteristic map is a graphical representation of the conditions and characteristics of the geology, geography, and hydrology of a coast. Making a coastal characteristic map with remote sensing requires several steps, namely: 1). satellite data collection: Pre-processing satellite data; 2). classification of satellite data; 3). satellite data analysis; and finally 4). making a coastal characteristic map. The area that is the fabric material is the beach of Indramayu Regency, West Java. Based on the analysis and interpretation of 2015 Landsat 8 (LDCM) image data, 2020 Sentinel 2 Image data, BATNAS, and DEMNAS data, it is known that the characteristics of the Indramayu beach are that it has geo-quaternary building materials which include: (a) coastal bunds, ( b) swamp deposits, (c) shallow marine deposits, (d) sand deposits, (e) flood plains, (f) river deposits, and (g) floodplain deposits. The morphology includes: (a) beach sand plains, (b) concave coastal swamps, (c) old beach bunds, (d) young beach bunds, (e) alluvial plains, (f) flood plains, and (g) fan plains delta. Finally, it has a distribution of surface sediments on the seabed which includes: (a) sand, (b) silt, gravelly sand, (d) slightly gravelly silt, (e) slightly gravelly sandy mud, and (f) gravelly mud.
RACISMO RECREATIVO NOS CORPOS-TERRITÓRIO DE ADOLESCENTES NEGRAS NA ESCOLA
Authors: Nascimento Erika Benigna , Souza Maria Celeste Reis Fernandes de , Paula Fernanda Cristina de 
Published: April 2023

O objetivo deste artigo é trazer à visibilidade a utilização do humor como mecanismo do racismo estrutural e as marcas deixadas nos corpos-território de adolescentes negras, estudantes da educação básica de uma escola pública da cidade de Governador Valadares — MG. O estudo apoiou-se em autores que discutem raça e autoras do feminismo negro, operando conceitualmente com racismo recreativo, interseccionalidade e corpo-território. O material empírico foi produzido em oficinas temáticas e as narrativas trazidas pelas 12 estudantes, participantes da pesquisa, foram analisadas por meio da análise episódica. Os episódios narrados denunciam as marcas nos corpos-território dessas adolescentes, assim como a naturalização de opressões de raça e gênero, instrumentalizadas pelo racismo recreativo que comparece no cotidiano escolar. Esse comparecimento cria e reafirma estereótipos sobre a categoria “mulher negra”, provoca desejos de afastamento da escola para não serem alvo de “piadas” e/ou “brincadeiras”, e questionamentos sobre sua própria humanidade, ao refletirem se suas aparências se assemelham aos animais utilizados para “xingá-las”. As adolescentes denunciam os silenciamentos da escola frente a esse tipo de “humor”, tensionando a sua naturalização e nos provocam, como educadores/as, a posicionarmos contra esses silenciamentos que engendram exclusões e a naturalização do racismo, entre outros mecanismos, os disfarçados de humor, mas que precisam ser nomeados como racismo recreativo.
DIFFERENCES OF RIB FRACTURES IN BLUNT TRAUMA PATIENTS ACCORDING TO AGE AND GENDER: A COMPUTED TOMOGRAPHY STUDY
Authors: TORUN Ebru , YÜKSEL Yavuz 
Published: April 2023

Objective
To investigate the difference between rib fractures
according to age and sex in blunt trauma patients.
Material and Method
The patients were classified into 3 age groups:
Group-1:18-44 years, Group-2:45-69 years, and
Group-3:70 years or more. Rib fractures were
classified into 3 groups based on their level on the
coronal plane (upper (1st-4th ribs), medium (5th-
8th ribs) and lower (9th-12th ribs)) and axial plane
(anterior, lateral and posterior).
Results
Rib fractures were found to be more common in male
(69%) to female (53%) (p=0.002). The incidence of
fractures was seen to increase with age (p=0.001;
r=615). Rib fractures were most commonly found in
the middle ribs (5th-8th ribs) in all-age-groups. The
incidence of fractures in the upper ribs was significantly
lower in the advanced age than the other age groups
(p=0.002). Fractures were least commonly found in the
anterior part of the rib in all age groups. Rib fractures
were observed at a higher rate in the lateral part in
young adults unlike the other age groups (p=0.001).
A significant difference was found between the age
groups in favor of young adults (group 1) in terms of
the presence of parenchymal contusion without rib
fracture (p=0.014).
Conclusion
Rib fracture was seen at a higher rate in male than
female in blunt thoracic trauma patients. Fractures
possibility of in the upper rib structures is lower in the
advanced age group. Unlike other groups, in young
people, a higher rate of fractures was detected in
the lateral part of the costa. One should be aware of
the possibility of parenchymal contusion without a rib
fracture in the young age group.
Seismoacoustic Monitoring of a Longwall Face Using Distributed Acoustic Sensing
Authors: Chambers Derrick , Shragge Jeffrey 
Published: April 2023

ABSTRACT
Violent, dynamic failures of rockmasses in underground mines pose significant hazards to workers and operations. Over the past several decades, hardrock mines have widely adopted seismic monitoring to help address such risks. However, coal mines, particularly those employing the longwall mining method, have struggled to implement similar monitoring strategies. This is because typical longwall mines are much larger and mine more rapidly than hardrock mines. Moreover, regulations place significant restrictions on the subsurface use of electronics in coal mines due to potentially explosive atmospheres. We present a new monitoring concept that uses distributed acoustic sensing (DAS) to turn an entire longwall face into a seismoacoustic array. After exploring the acoustic response of our sensors in the laboratory, we deployed the array at an active underground longwall mine for several days. We examine 33 events recorded by both the in-mine DAS array and a surface seismic network. We observed that the array records both seismic vibrations traveling through rock and mining equipment as well as sound waves propagating in the workings. We show that waveform moveouts are clearly visible, and that the standard deviation of the audio recordings is a straightforward yet promising metric that could help quantify burst damage. Although improvements are needed before mines can routinely use this monitoring strategy, DAS-based seismoacoustic arrays may assist in understanding coal-burst mechanisms and managing associated risks in underground longwall mines as well as enable better understanding of damage associated with dynamic failures in other underground environments.
Evolution of the cluster development concept: from agglomeration theory to ecosystems
Authors: Gamidullaeva L. A. , Strakhov E. P. 
Published: April 2023

Purpose: of the study is to substantiate the relationship between cluster theory and ecosystem concepts on the basis of systematization of scientific research directions on the problem of economic clustering.Methods: the study is based on the theories of regional, spatial economics, innovative development, network and ecosystem economics, presented in the works of the Russian and foreign scientists. To solve the tasks set, general scientific methods (analysis, synthesis, description, comparison, induction, historical, classification, etc.) and special methods (bibliometric and content analysis of literature, statistical) were used. In order to conduct bibliometric analysis, a standardized methodology for exploratory analytics was applied using the tools provided in modern computer data analysis packages.Results: on the basis of thematic selections from the Scopus database, the systematization of the most cited scientific publications on the problem of cluster development, published for the period 1997-2022 was carried out. A qualitative content analysis of the content of text arrays, followed by a meaningful interpretation of the identified patterns, made it possible to group the main directions of scientific thought in this area and identify promising areas for the further research. A conceptual scheme for the evolution of the formation and development of the cluster concept: the key theories and approaches that determined the trajectory of its development are identified.Conclusions and Relevance: the hypothesis that the ecosystem concept is an evolutionary development of cluster theory is confirmed. It is concluded that in the future the topic of innovative ecosystems is highly likely to move into the category of the basic ones devoted to cluster development. Despite the similarity (in the first approximation) of clusters and ecosystems, these approaches differ fundamentally in many criteria, primarily in terms of the proposed mechanisms for building interaction between participants, organization and regulation. In order to use the ecosystem approach in the practice of regional and industrial policy, its further conceptual, methodological and instrumental support is necessary.