bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide DNA methylation and transcriptome analyses reveal the key gene for wool type variation in sheep
Authors: Wang Jiankui , Hua Guoying , Cai Ganxian , Ma Yuhao , Yang Xue , Zhang Letian , Li Rui , Liu Jianbin , Ma Qing , Wu Keliang , Zhao Yaofeng , Deng Xuemei 
Published: July 2023

Abstract
Background
Wool fibers are valuable materials for textile industry. Typical wool fibers are divided into medullated and non-medullated types, with the former generated from primary wool follicles and the latter by either primary or secondary wool follicles. The medullated wool is a common wool type in the ancestors of fine wool sheep before breeding. The fine wool sheep have a non-medullated coat. However, the critical period determining the type of wool follicles is the embryonic stage, which limits the phenotypic observation and variant contrast, making both selection and studies of wool type variation fairly difficult.

Results
During the breeding of a modern fine (MF) wool sheep population with multiple-ovulation and embryo transfer technique, we serendipitously discovered lambs with ancestral-like coarse (ALC) wool. Whole-genome resequencing confirmed ALC wool lambs as a variant type from the MF wool population. We mapped the significantly associated methylation locus on chromosome 4 by using whole genome bisulfite sequencing signals, and in turn identified the SOSTDC1 gene as exons hypermethylated in ALC wool lambs compare to their half/full sibling MF wool lambs. Transcriptome sequencing found that SOSTDC1 was expressed dozens of times more in ALC wool lamb skin than that of MF and was at the top of all differentially expressed genes. An analogy with the transcriptome of coarse/fine wool breeds revealed that differentially expressed genes and enriched pathways at postnatal lamb stage in ALC/MF were highly similar to those at the embryonic stage in the former. Further experiments validated that the SOSTDC1 gene was specifically highly expressed in the nucleus of the dermal papilla of primary wool follicles.

Conclusion
In this study, we conducted genome-wide differential methylation site association analysis on differential wool type trait, and located the only CpG locus that strongly associated with primary wool follicle development. Combined with transcriptome analysis, SOSTDC1 was identified as the only gene at this locus that was specifically overexpressed in the primary wool follicle stem cells of ALC wool lamb skin. The discovery of this key gene and its epigenetic regulation contributes to understanding the domestication and breeding of fine wool sheep.
Role of innate immunological/inflammatory pathways in myelodysplastic syndromes and AML: a narrative review
Authors: Vegivinti Charan Thej Reddy , Keesari Praneeth Reddy , Veeraballi Sindhusha , Martins Maia Catarina Maria Pina , Mehta Ansh Krishnachandra , Lavu Rohit Reddy , Thakur Rahul Kumar , Tella Sri Harsha , Patel Riya , Kakumani Venkata Kiranmayi , Pulakurthi Yashwitha Sai , Aluri Srinivas , Aggarwal Ritesh Kumar , Ramachandra Nandini , Zhao Rongbao , Sahu Srabani , Shastri Aditi , Verma Amit 
Published: July 2023

AbstractDysregulation of the innate immune system and inflammatory-related pathways has been implicated in hematopoietic defects in the bone marrow microenvironment and associated with aging, clonal hematopoiesis, myelodysplastic syndromes (MDS), and acute myeloid leukemia (AML). As the innate immune system and its pathway regulators have been implicated in the pathogenesis of MDS/AML, novel approaches targeting these pathways have shown promising results. Variability in expression of Toll like receptors (TLRs), abnormal levels of MyD88 and subsequent activation of NF-κβ, dysregulated IL1-receptor associated kinases (IRAK), alterations in TGF-β and SMAD signaling, high levels of S100A8/A9 have all been implicated in pathogenesis of MDS/AML. In this review we not only discuss the interplay of various innate immune pathways in MDS pathogenesis but also focus on potential therapeutic targets from recent clinical trials including the use of monoclonal antibodies and small molecule inhibitors against these pathways.
Using the collaborative cross to identify the role of host genetics in defining the murine gut microbiome
Authors: Nagarajan Aravindh , Scoggin Kristin , Gupta Jyotsana , Threadgill David W. , Andrews-Polymenis Helene L. 
Published: July 2023

Abstract
Background
The human gut microbiota is a complex community comprised of trillions of bacteria and is critical for the digestion and absorption of nutrients. Bacterial communities of the intestinal microbiota influence the development of several conditions and diseases. We studied the effect of host genetics on gut microbial composition using Collaborative Cross (CC) mice. CC mice are a panel of mice that are genetically diverse across strains, but genetically identical within a given strain allowing repetition and deeper analysis than is possible with other collections of genetically diverse mice.

Results
16S rRNA from the feces of 167 mice from 28 different CC strains was sequenced and analyzed using the Qiime2 pipeline. We observed a large variance in the bacterial composition across CC strains starting at the phylum level. Using bacterial composition data, we identified 17 significant Quantitative Trait Loci (QTL) linked to 14 genera on 9 different mouse chromosomes. Genes within these intervals were analyzed for significant association with pathways and the previously known human GWAS database using Enrichr analysis and Genecards database. Multiple host genes involved in obesity, glucose homeostasis, immunity, neurological diseases, and many other protein-coding genes located in these regions may play roles in determining the composition of the gut microbiota. A subset of these CC mice was infected with Salmonella Typhimurium. Using infection outcome data, an increase in abundance of genus Lachnospiraceae and decrease in genus Parasutterella correlated with positive health outcomes after infection. Machine learning classifiers accurately predicted the CC strain and the infection outcome using pre-infection bacterial composition data from the feces.

Conclusion
Our study supports the hypothesis that multiple host genes influence the gut microbiome composition and homeostasis, and that certain organisms may influence health outcomes after S. Typhimurium infection.
The Mammoth prophecies: a role-playing game on controversies around a socio-technical innovation and its effects on students’ capacities to think about the future
Authors: Dayé Christian , Prunč Roman Lukas , Hofmann-Wellenhof Martin 
Published: July 2023

AbstractThis article reports on a political game played between November 2021 and February 2022 at a European university in the frame of an elective course open to students from all disciplines. It started from a scenario that combined a real and ongoing innovation process—the use of CRISPR/Cas9 technology to introduce specific genes from unfrozen mammoths onto an elephant species to make it more resistant to cold—with a fictive joint project between a US-based company and the Russian government to release those mammoths on an island in the North-West of Russia. Almost no rules were given, and the students were distributed in nine groups to represent different actors deemed relevant to the scenario. These groups were the national governments of (1) Russia, (2) close neighboring states, (3) European countries farther away from the project location; (4) the company working on the innovation, dubbed HELIOS; (5) other market actors; civil society organizations concerned with (6) animal rights and (7) the environment; (8) the European Commission and the EU Parliament; and (9) media. The game was led by the course teacher and two student volunteers. Based on the results of both qualitative and quantitative forms of ex-post evaluation, we assess whether the Mammoth game meets the expectations that guided the game design process. Furthermore, we discuss whether the Mammoth game had a positive effect on students’ abilities to think about the future.
A re-assessment of historical records pertaining to the activity of Mt. Baekdu (Paektu, Tianchi) volcano
Authors: Yun Sung-Hyo , Lee Jeonghyun , Chang Cheolwoo , Oppenheimer Clive 
Published: July 2023

AbstractThis study re-assesses the historical records pertaining to the activity of Mt. Baekdu according to volcanic phenomena. We categorized volcanic phenomena into five categories: rumbling, atmospheric abnormality, ash rain, ash cloud and phenomenon sightings, and investigated historical records (in Chinese) for each phenomenon and identified their volcanological implications. Among the volcanic phenomena, ash rain had the most abundant records, and in particular, Goryeosa recorded the ash rain phenomenon 56 times. And more than 90 volcanic eruptions were discovered from the Millennium Eruption from November 3, 946 AD, to February 7, 947 AD, most of which were either Plinian or Vulcanian eruptions with volcanic ash dispersed into the regions surrounding the volcano creating fallout ash. Based on the historical eruptions, eruption precursors, and volcanic unrest of the volcano between 2002 and 2006, Mt. Baekdu is regarded as an active volcano that has the potential to erupt. Therefore, to mitigate the hazard caused by the eruption of Mt. Baekdu, it is necessary to analyze the historical eruption records of Mt. Baekdu and understand the characteristics of the eruptions through this analysis.
Hyperthermophilic pretreatment composting can reduce ammonia emissions by controlling proteolytic bacterial community and the physicochemical properties
Authors: Huang Ying , Chen Yuehong , Huang Hongying , Shah Ghulam Mustafa , Lin Jiujun , Yan Meiling , Guo Chengbao , Xiao Xu 
Published: July 2023

AbstractProteolysis is the rate-limiting step in the mineralization of organic nitrogen into ammonium (NH4+) and thereby the ammonia (NH3) released during the composting. However, the dynamics of bacterial proteolytic communities related to NH3 emissions during the composting systems are mostly unknown. This study aimed to examine and compare the effects of hyperthermophilic pretreatment composting (HPC) and traditional composting (TC) methods on (i) the difference of NH3 loss and nitrogenous compounds; (ii) the dynamics of the proteolytic bacterial community involved in the proteolysis and (iii) the correlation between the proteolytic bacterial community, biophysiochemical characteristics and NH3 loss. Results revealed that the HPC decreased NH3 loss by 42% as compared to TC during 60-day composting period. This was accompanied with an inhibitory effect on protease activity in the HPC where the relative abundances of the proteolytic bacteria (Bacillus megaterium and Staphylococcus cohnii) were reduced significantly as compared to TC. Partial least-squares path modeling suggested that various physicochemical properties such as higher temperature as well as lower C/N ratio during composting played a dominant role in affecting the abundance of proteolytic bacteria, which may have been an important factor contributing to the lower NH3 loss in HPC. All these findings lead us to conclude that the HPC can significantly reduce NH3 loss by inhibiting the proteolytic bacteria and protease activity responsible for NH3 release.
Graphical Abstract
Rapid and robust squashed spore/colony PCR of industrially important fungi
Authors: Yuan Guoliang , Czajka Jeffrey J. , Dai Ziyu , Hu Dehong , Pomraning Kyle R. , Hofstad Beth A. , Kim Joonhoon , Robles Ana L. , Deng Shuang , Magnuson Jon K. 
Published: July 2023

AbstractBackgroundFungi have been utilized for centuries in medical, agricultural, and industrial applications. Development of systems biology techniques has enabled the design and metabolic engineering of these fungi to produce novel fuels, chemicals, and enzymes from renewable feedstocks. Many genetic tools have been developed for manipulating the genome and creating mutants rapidly. However, screening and confirmation of transformants remain an inefficient step within the design, build, test, and learn cycle in many industrial fungi because extracting fungal genomic DNA is laborious, time-consuming, and involves toxic chemicals.ResultsIn this study we developed a rapid and robust technique called “Squash-PCR” to break open the spores and release fungal genomic DNA as a template for PCR. The efficacy of Squash-PCR was investigated in eleven different filamentous fungal strains. Clean PCR products with high yields were achieved in all tested fungi. Spore age and type of DNA polymerase did not affect the efficiency of Squash-PCR. However, spore concentration was found to be the crucial factor for Squash-PCR inAspergillus niger, with the dilution of starting material often resulting in higher PCR product yield. We then further evaluated the applicability of the squashing procedure for nine different yeast strains. We found that Squash-PCR can be used to improve the quality and yield of colony PCR in comparison to direct colony PCR in the tested yeast strains.ConclusionThe developed technique will enhance the efficiency of screening transformants and accelerate genetic engineering in filamentous fungi and yeast.
mise en discours de la mode durable via Instagram : regard sur les marques Marine Serre, Ganni et Boohoo
Authors: Creux-Martelli Ambre , Yates Stéphanie 
Published: July 2023

Afin de répondre à l’impératif de durabilité, les marques du secteur de la mode, une industrie particulièrement polluante, développent un discours visant à mettre en valeur leur démarche écoresponsable. Dans cet article, nous nous intéressons aux discours déployés sur Instagram par trois marques, qui correspondent à différents modèles d’affaires de l’industrie : Marine Serre, fondamentalement responsable; Ganni, une marque en transition écoresponsable; et Boohoo, une marque associée à l’ultra fast fashion. À partir d’une analyse des différentes thématiques privilégiées dans les quelque 171 publications de ces trois marques au cours de l’automne 2021, nous concluons que si les marques correspondant aux deux premiers idéaux-types semblent se conformer aux critères d’authenticité reconnus en matière de communication environnementale, la marque de fast fashion, sans grande surprise, verse dans l’écoblanchiment. Or, nos résultats font ressortir que cet écoblanchiment prend une forme insidieuse, qui s’éloigne des clichés habituels : de fait, la marque renverse le fardeau de l’écoresponsabilité en le transférant à ses consommateurs. Nous concluons en soulignant que cette nouvelle forme de purpose washing n’est pas sans conséquence pour l’ensemble des acteurs de l’industrie, y compris ceux qui souhaitent développer une approche authentiquement durable.
N‐acetylcysteine ameliorates hematuria‐associated tubulointerstitial injury in 5/6 nephrectomy mice
Authors: Medipally Ajay , Xiao Min , Satoskar Anjali A. , Biederman Laura , Dasgupta Alana , Ivanov Iouri , Mikhalina Galina , Rovin Brad , Brodsky Sergey V. 
Published: July 2023

AbstractChronic kidney disease (CKD) is characterized by increased interstitial fibrosis and tubular atrophy (IFTA) in the kidney. Chronic hematuria is a hallmark of several human kidney diseases and often is seen in patients on anticoagulation therapy. We had previously demonstrated that chronic hematuria associated with warfarin increases IFTA in 5/6 nephrectomy (5/6NE) rats, and such treatment increases reactive oxygen species (ROS) in the kidney. The goal of this study was to evaluate the effects of the antioxidant N‐acetylcysteine (NAC) on the progression of IFTA in 5/6NE mice. 5/6NE C57BL/6 and 5/6NE 129S1/SvImJ mice were treated with warfarin alone or with warfarin and NAC for 23 weeks. Serum creatinine (SCr), hematuria, blood pressure (BP), and ROSs in the kidney were measured; kidney morphology was evaluated. Warfarin doses were titrated to achieve prothrombin time (PT) increase to the levels seen with therapeutic human doses. Warfarin treatment resulted in an increased SCr, systolic BP, hematuria, expression of TGF‐ß and ROS in the kidney in both mouse strains. Tumor necrosis factor alpha (TNF‐ɑ) levels in the serum were increased in 5/6NE mice treated with warfarin. IFTA was increased as compared with control 5/6NE mice, and this increase in IFTA was more prominent in 129S1/SvImJ than in C57BL/6 mice. NAC ameliorated the warfarin‐associated increase in SCr and BP but not hematuria. IFTA, TGF‐ß, and ROS in the kidney as well as TNF‐ɑ levels in the serum were reduced in mice treated with NAC and warfarin as compared to mice treated with warfarin alone. NAC mitigates the increase in SCr and IFTA in mice with chronic hematuria by reducing oxidative stress in the kidney. This data open novel possibilities for treatments in CKD patients.
The effect of hormonal contraceptive therapy on clinical laboratory parameters: a literature review
Authors: Özcan Ömer , den Elzen Wendy P.J. , Hillebrand Jacquelien J. , den Heijer Martin , van Loendersloot Laura L. , Fischer Johan , Hamer Henrike , de Jonge Robert , Heijboer Annemieke C. 
Published: July 2023

Abstract
Hormonal contraceptives (HC) are widely used among women in reproductive ages. In this review, the effects of HCs on 91 routine chemistry tests, metabolic tests, and tests for liver function, hemostatic system, renal function, hormones, vitamins and minerals were evaluated. Test parameters were differently affected by the dosage, duration, composition of HCs and route of administration. Most studies concerned the effects of combined oral contraceptives (COC) on the metabolic, hemostatic and (sex) steroids test results. Although the majority of the effects were minor, a major increase was seen in angiotensinogen levels (90–375 %) and the concentrations of the binding proteins (SHBG [∼200 %], CBG [∼100 %], TBG [∼90 %], VDBP [∼30 %], and IGFBPs [∼40 %]). Also, there were significant changes in levels of their bound molecules (testosterone, T3, T4, cortisol, vitamin D, IGF1 and GH). Data about the effects of all kinds of HCs on all test results are limited and sometimes inconclusive due to the large variety in HC, administration routes and dosages. Still, it can be concluded that HC use in women mainly stimulates the liver production of binding proteins. All biochemical test results of women using HC should be assessed carefully and unexpected test results should be further evaluated for both methodological and pre-analytical reasons. As HCs change over time, future studies are needed to learn more about the effects of other types, routes and combinations of HCs on clinical chemistry tests.
Epidemiologic Features of Human Rabies in China from 2015-2021
Authors: Zhang Na , Song Chengqian , Tao Xiaoyan , Zhu Wuyang 
Published: July 2023

Objective:
This study aimed to enhance the current understanding of the epidemiologic characteristics, laboratory diagnostic levels, and changes in pathogenic populations of rabies in China by studying the status of the human rabies epidemic in China from 2015-2021 and provide useful information for guiding rabies disease prevention and control strategies.

Methods:
We analyzed the incidence, distribution, and laboratory testing of human rabies in mainland China using statutory surveillance data from 2015-2021. Based on a literature review, the study summarizes the recent updates of the rabies virus population in each province based on previous monitoring.

Results:
A total of 3032 rabies cases were reported in China from 2015-2021, with a year-after-year decrease in the total number of cases. Most of the cases (75.19%) were distributed in Hunan, Henan, Guangxi, Guizhou, Hubei, Yunnan, Jiangsu, Anhui, Guangdong, and Sichuan, with 13 counties (districts) reporting > 50 cases in 7 years. The number of reported counties (districts) decreased from 512 in 2015 to 116 in 2021. Farmers accounted for most of the cases (73%), and the highest proportion of cases (54.62%) occurred in individuals 50-75 years of age. No changes in endemic populations were detected in China. The laboratory diagnosis rate of cases increased from 4.74% in 2015 to 22.93% in 2021.

Conclusions:
The rabies epidemic in China decreased steadily from 2015-2021, with a marked contraction in the geographic scope. In the future it will be necessary to continue to carry out large-scale dog immunization and strengthen the surveillance and laboratory diagnosis of rabies.
DIVERSIDADE, DIREITOS HUMANOS E CURRICULO ESCOLAR
Authors: Melo Jose Wilson Rodrigues de 
Published: July 2023

A mundialização ressignificou o <<local>>. Contudo, os efeitos da globalização econômica materializam-se na forma de desemprego, fome, ampliação das desigualdades sociais. Este contexto traz os desafios dos direitos humanos no Brasil. Deste objeto veio a questão: qual é a importância das políticas e práticas curriculares para o desenvolvimento de uma cultura dos direitos humanos? O objetivo do artigo foi contrastar a relação da diversidade, dos direitos e o currículo escolar. A metodologia utilizada foi de base bibliográfica. Foram levantados artigos em “motores de busca” da internet (CAPES, scielo Brasil, google scholar) e na biblioteca do autor. O artigo foi estruturado em três seções. A primeira aborda aspectos da diversidade cultural brasileira. A segunda destaca os direitos humanos articulados com a educação para a igualdade e a dignidade. Por último frisou-se o currículo escolar para vivenciar os direitos humanos na diversidade cultural. Este artefato cultural pode tornar-se o embolo de valores para uma cultura dos direitos humanos
“TODOS TÊM A MESMA COR”?
Authors: Carvalho Liandra Lima , Ramos Márcia Ribeiro 
Published: July 2023

O presente ensaio buscou apresentar um levantamento acerca da produção científica das pós-graduações scricto sensu em psicologia (Dissertações de Mestrado e Teses de Doutorado), sobre branquitude, defendidas de 2003 a 2018 no Brasil, visando perceber, dessa forma, como a psicologia vem se posicionando no enfrentamento ao racismo brasileiro. Para tal utilizou com base os principais bancos de dados e plataformas digitais: Sucupira, BDTD, Portal Domínio Público e o Google acadêmico; e teve como resultado uma ínfima produção, que foi analisada de forma contundente. As descobertas da pesquisa fizeram com que fosse observada a necessidade da categoria profissional, especialmente, por meio dos cursos de pós-graduação, com a realização de pesquisas, aprofundar suas análises sobre o racismo no Brasil, os impactos dele na vida da população negra e como a psicologia pode atuar visando o combate ao racismo, bem como o fortalecimento de denúncias, o apoio aos movimentos sociais voltados a essa causa e especialmente, na garantia dos direitos humanos.
#BNCC:
Authors: Dutra-Pereira Franklin Kaic , Tinôco Saimonton 
Published: July 2023

Hashtag BNCC... Eis o que nos move a escrever uma carta a quem pesquisa sobre as políticas curriculares no Brasil, em meio ao avanço da utilização de plataformas virtuais de interação – chamadas comumente de redes sociais –, sobretudo no contexto da pandemia de COVID-19. Arriscamo-nos num processo dialógico e inventivo de fazer pesquisa COM, criando, apresentando e movimentando questões a partir de postagens publicadas no Twitter, as quais nomeamos de e-narrativas. Tais escolhas se devem ao fato de considerarmos que os tuites têm algo a dizer sobre o acontecido nos espaços onde se faz a educação, por isso podem ser entendidos como narrativas que contêm pistas para estudos curriculares. Também fomos provocados pelos debates gerados no IX Colóquio Internacionais de Políticas e Práticas Curriculares, organizado de forma remota pelo Grupo de Estudos e Pesquisas em Políticas Curriculares, em 2020. Com isso, pretendemos chamar a atenção da comunidade científica para questões sinalizadas nas postagens – que traduzem anúncios e denúncias e inquietações e dúvidas e desejos e... –, bem como para a necessidade de (re)pensarmos as escolhas que temos feito quanto ao diálogo e a comunicação de nossas pesquisas, sobretudo no que se refere às comunidades escolares e aos cursos de formação docente.