bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical Pollution and Regulatory Choices at the Start of Industrialization: Comparing France and Great Britain
Authors: Morag-Levine Noga 
Published: April 2023

Abstract
In both Britain and France, pollution from emergent chemical manufacturing during the early industrial era presented a choice between two regulatory approaches. One option, consistent with longstanding restrictions in both countries on the location of malodorous trades, insisted on the separation of chemical plants from (upper-class) residences. The alternative approach allowed polluting firms to operate near residences, subject to incremental technology-based mitigation. By 1810, France issued a decree that conferred on most chemical manufacturers the right to operate inside cities, subject to permitting requirements. For residents of working-class industrial neighborhoods, who never stood a realistic chance of removing polluters, a regulatory regime geared at incremental mitigation held the potential for modest environmental improvement. For wealthy landowners, however, partial technological mitigation was far inferior to the complete relief obtainable through the removal of pollution sources.
France’s example loomed large over chemical pollution debates in nineteenth-century Britain. Manufacturers hoped the courts would remove locational restrictions on chemical plants, while the near absence of pollution mitigation within working-class areas alarmed liberal reformers. The road to a compromise solution patterned after France’s was impeded in Britain, however, by a deep-seated aversion to uniform, centralized pollution control. This reticence was rooted in common-law-inspired understandings of nuisance law as the sole and inviolable constitutional means for the regulation of land use. By the 1860s, through the combined impact of St. Helens v. Tipping (1865) and the Alkali Act of 1863, Britain moved towards the French approach. Nevertheless, compared with France, British law remained more protective of landowners, more reactive in its implementation, and more willing to vary required mitigation based on sociodemographic factors. Beyond its contribution to comparative environmental history, in revealing the legal-ideological underpinnings of the Franco-British divide over the regulation of early chemical pollution, this Article also seeks to shine a light on the lingering role of legal ideology within contemporary cross-national divisions over the efficacy and legitimacy of centralized technology-based regulatory instruments
Populations of unlabelled networks: graph space geometry and generalized geodesic principal components
Authors: Calissano Anna , Feragen Aasa , Vantini Simone 
Published: April 2023

Abstract
Statistical analysis for populations of networks is widely applicable, but challenging, as networks have strongly non-Euclidean behaviour. Graph space is an exhaustive framework for studying populations of unlabelled networks that are weighted or unweighted, uni- or multilayered, directed or undirected. Viewing graph space as the quotient of a Euclidean space with respect to a finite group action, we show that it is not a manifold, and that its curvature is unbounded from above. Within this geometrical framework we define generalized geodesic principal components, and we introduce the align-all-and-compute algorithms, all of which allow for the computation of statistics on graph space. The statistics and algorithms are compared with existing methods and empirically validated on three real datasets, showcasing the potential utility of the framework. The whole framework is implemented within the geomstats Python package.
Maximizing the benefits from sharing cyber threat intelligence by government agencies and departments
Authors: Dykstra Josiah , Gordon Lawrence A , Loeb Martin P , Zhou Lei 
Published: April 2023

AbstractThe primary objective of the current study is to analytically examine the economic benefits an organization can obtain by receiving and processing cyber threat intelligence (CTI) shared by the US government. Our results show that the benefits from receiving CTI are closely associated with the difference between the threat level indicated by the CTI and the receiving organization’s prior belief of the threat level. In addition, for the same difference between the threat levels indicated by the CTI and the organization’s prior belief, our analyses show that the magnitude of adjustments to an organization’s cybersecurity investments is inversely related to the organization’s prior belief of the threat level. Thus, larger benefits can be obtained when the receiving organization’s prior belief of a threat level is lower. Taken together, our results suggest that the common belief that it is optimal for a federal government agency or department to focus on sharing CTI related to vulnerabilities with the highest threat level is misguided. More generally, the benefits from CTI sharing can be improved if producers of CTI could develop a clearer understanding of the prior beliefs that organizations have concerning their threat level and focus on sharing CTI that is significantly different from those prior beliefs.
The genome sequence and demographic history of Przewalskia tangutica (Solanaceae), an endangered alpine plant on the Qinghai–Tibet Plateau
Authors: Wu Ying , Yang Jiao , Yang Yongzhi , Liu Jianquan 
Published: April 2023

Abstract
To adapt to high-altitude habitats, many alpine plants develop self-compatible breeding systems from outcrossing. The genetic bases for this shift and the resulting demographic consequences remain largely unexplored. Here, we present a high-quality, chromosome-level genome assembly of the monotypic and endangered alpine perennial Przewalskia tangutica (Solanaceae) occurring on the Qinghai–Tibet Plateau (QTP). Our assembled genome is approximately 3 Gb, with a contig N50 size of 17 Mb, and we identified one lineage-specific whole-genome duplication. We found that the gametophytic self-incompatibility (GSI) syntenic locus to the other obligate outcrossing Solanaceae species was broken by the inserted the long terminal repeats, and changes in the flower-specific expression of the homologous genes, and the linked GSI genes in this species. Such changes may have led to its self-compatibility. We identified three deeply diverged lineages in the central distribution of this species, and the gene flow between them was weak but continuous. All three lineages diverged and decreased their population sizes since the largest glaciations occurred in the QTP approximately 720–500 thousand years ago. In addition, we identified one obvious hybrid population between two lineages, suggesting that genetic exchanges between and within lineages still occur. Our results provide insights into evolutionary adaptation through facultative self-pollination and demographic consequences of this alpine rare species in arid habitats.
An enhanced recovery programme significantly improves postoperative outcomes after surgical aortic valve replacement
Authors: Giger Albane , Schneider Clément , Marguerite Sandrine , Ramlugun Dharmesh , Maechel Anne-Lise , Collange Olivier , Mertes Paul-Michel , Mazzucotelli Jean-Philippe , Kindo Michel 
Published: April 2023

Abstract

OBJECTIVES
Evidence regarding the benefits of an enhanced recovery after cardiac surgery (ERACS) programme is lacking. The aim of this study was to analyse the impact of a systematic standardized ERACS programme for patients undergoing isolated elective surgical aortic valve replacement (SAVR) for aortic stenosis in terms of hospital mortality and morbidity, patient blood management and length of stay.

METHODS
Patients undergoing isolated elective SAVR for aortic stenosis between 2015 and 2020 were identified from our database (n = 941). The standardized systematic ERACS programme was implemented in November 2018. Propensity score matching indicated that 259 patients would receive standard perioperative care (control group) and 259 patients would receive the ERACS programme (ERACS group). The primary outcome was hospital mortality. The secondary outcomes were hospital morbidity, patient blood management and length of stay.

RESULTS
Both groups had similar hospital mortality rates (0.4%). The ERACS group had a significantly lower troponin I peak level (P &lt; 0.001), a larger proportion of improved perioperative left ventricular ejection fractions (P = 0.001), a lower incidence of bronchopneumonia (P = 0.030), a larger proportion of patients with mechanical ventilation &lt;6 h (P &lt; 0.001), a lower incidence of delirium (P = 0.028) and less acute renal failure (P = 0.013). The ERACS group had a significantly lower rate of red blood cell transfusions (P = 0.002). The intensive care unit stay was significantly shorter in the ERACS group than in the control group (P = 0.039).

CONCLUSIONS
The standardized systematic ERACS programme significantly improved postoperative outcomes and should become the reference for the perioperative care pathway for patients undergoing SAVR.
Proteomic cardiovascular risk assessment in chronic kidney disease
Authors: Deo Rajat , Dubin Ruth F , Ren Yue , Murthy Ashwin C , Wang Jianqiao , Zheng Haotian , Zheng Zihe , Feldman Harold , Shou Haochang , Coresh Josef , Grams Morgan , Surapaneni Aditya L , Bhat Zeenat , Cohen Jordana B , Rahman Mahboob , He Jiang , Saraf Santosh L , Go Alan S , Kimmel Paul L , Vasan Ramachandran S , Segal Mark R , Li Hongzhe , Ganz Peter 
Published: April 2023

Abstract

Aims
Chronic kidney disease (CKD) is widely prevalent and independently increases cardiovascular risk. Cardiovascular risk prediction tools derived in the general population perform poorly in CKD. Through large-scale proteomics discovery, this study aimed to create more accurate cardiovascular risk models.

Methods and results
Elastic net regression was used to derive a proteomic risk model for incident cardiovascular risk in 2182 participants from the Chronic Renal Insufficiency Cohort. The model was then validated in 485 participants from the Atherosclerosis Risk in Communities cohort. All participants had CKD and no history of cardiovascular disease at study baseline when ∼5000 proteins were measured. The proteomic risk model, which consisted of 32 proteins, was superior to both the 2013 ACC/AHA Pooled Cohort Equation and a modified Pooled Cohort Equation that included estimated glomerular filtrate rate. The Chronic Renal Insufficiency Cohort internal validation set demonstrated annualized receiver operating characteristic area under the curve values from 1 to 10 years ranging between 0.84 and 0.89 for the protein and 0.70 and 0.73 for the clinical models. Similar findings were observed in the Atherosclerosis Risk in Communities validation cohort. For nearly half of the individual proteins independently associated with cardiovascular risk, Mendelian randomization suggested a causal link to cardiovascular events or risk factors. Pathway analyses revealed enrichment of proteins involved in immunologic function, vascular and neuronal development, and hepatic fibrosis.

Conclusion
In two sizeable populations with CKD, a proteomic risk model for incident cardiovascular disease surpassed clinical risk models recommended in clinical practice, even after including estimated glomerular filtration rate. New biological insights may prioritize the development of therapeutic strategies for cardiovascular risk reduction in the CKD population.
COVID-19 vaccination and changes in preventive behaviours: findings from the 2021 vaccine roll-out in Switzerland
Authors: Hitchman Sara C , Geber Sarah , Tribelhorn Lukas , Friemel Thomas N 
Published: April 2023

Abstract

Background
Behavioural, environmental, social and systems interventions (BESSIs) remain important for controlling the COVID-19 pandemic in addition to vaccination. However, people’s adoption of BESSIs may decrease as vaccination rates increase due to reductions in the perceived threat of disease, and changes in risk perceptions of behaviours that increase the chance of infection. Thus, we examined predictors of and changes over time in reports of mask wearing and physical distancing and whether changes in mask wearing and physical distancing differed by vaccination status during the main 2021 COVID-19 vaccine roll-out period in Switzerland.

Methods
Weekly online cross-sectional surveys (26 April 2021 to 1 August 2021) among people 18–79 years old in Switzerland, N = 6308 observations and 5511 cases. Logistic regression models using generalized estimating equations.

Results
Reports of being vaccinated increased, while mask wearing and physical distancing decreased over time. This decrease was similar regardless of vaccination status. However, the level of reported mask wearing and physical distancing remained higher among vaccinated people. Older, female, and Italian language region respondents also had higher odds of reporting mask wearing and physical distancing.

Conclusions
Adoption of COVID-19 preventive behaviours is associated with demographics and vaccination status. Further research is needed to understand the reasons why people who are not vaccinated are less likely to adopt preventive behaviours, including that they may have fewer social and environmental opportunities to do so.
Comparative study of GBP recruitment on two cytosol-dwelling pathogens, Francisella novicida and Shigella flexneri highlights differences in GBP repertoire and in GBP1 motif requirements
Authors: Valeva Stanimira V , Degabriel Manon , Michal Fanny , Gay Gabrielle , Rohde John R , Randow Felix , Lagrange Brice , Henry Thomas 
Published: April 2023

Abstract
Guanylate-Binding Proteins are interferon-inducible GTPases that play a key role in cell autonomous responses against intracellular pathogens. Despite sharing high sequence similarity, subtle differences among GBPs translate into functional divergences that are still largely not understood. A key GBP feature is the formation of supramolecular GBP complexes on the bacterial surface. Such complexes are observed when GBP1 binds lipopolysaccharide (LPS) from Shigella and Salmonella and further recruits GBP2-4. Here, we compared GBP recruitment on two cytosol-dwelling pathogens, Francisella novicida and S. flexneri. Francisella novicida was coated by GBP1 and GBP2 and to a lower extent by GBP4 in human macrophages. Contrary to S. flexneri, F. novicida was not targeted by GBP3, a feature independent of T6SS effectors. Multiple GBP1 features were required to promote targeting to F. novicida while GBP1 targeting to S. flexneri was much more permissive to GBP1 mutagenesis suggesting that GBP1 has multiple domains that cooperate to recognize F. novicida atypical LPS. Altogether our results indicate that the repertoire of GBPs recruited onto specific bacteria is dictated by GBP-specific features and by specific bacterial factors that remain to be identified.
Seismic stratigraphy and attenuation of gas-hydrate zones within Hikurangi and Gondwana margins, eastern New Zealand
Authors: Wang Yichuan , Bedle Heather , Marfurt Kurt J 
Published: April 2023

SUMMARY
Gas hydrates that occur on many continental margins have received global attention. In reflection seismic imaging, the bottom-simulating reflector (BSR) is a common indicator of gas hydrates. However, it is difficult to identify gas hydrates and quantify their amounts through the BSR alone. For gas-hydrate characterization, it is therefore useful to measure seismic stratigraphic and attenuation attributes. Short-scale patterns of layering that contain information about the amount and mechanism of gas hydrates can be identified through stratigraphic and attenuation attributes. We measure the complete time-variant spectra by using sparse strongest peaks, and the spectral differences at different times through attenuation parameters Q–1 and γ. The traditional Q–1 is associated with the attenuation of the frequency-dependent part of wavefield, and the γ characterizes the frequency-independent attenuation. The measurement approach is straightforward and requires no sophisticated inverse algorithm and is applied to surface seismic data acquired over the Hikurangi and Gondwana margins, eastern New Zealand. High-quality spectral and attenuation images are obtained. Spectral attributes correlate with BSRs and large positive Q–1 and negative γ-values are below and above the BSRs, which are interpreted as being related to free-gas and gas-hydrate accumulations. These results will aid the quantification of gas hydrates and the assessment of their roles as an energy resource, as a potential geological hazard, and in climate change and ocean warming.
Substrate specificity and transglycosylation capacity of α-L-fucosidases across GH29 assessed by bioinformatics-assisted selection of functional diversity
Authors: Perna Valentina N , Barrett Kristian , Meyer Anne S , Zeuner Birgitte 
Published: April 2023

Abstract
Glycoside hydrolase family 29 (GH29) encompasses α-L-fucosidases, i.e. enzymes that catalyze the hydrolytic release of fucose from fucosylated glycans, including N- and O-linked glycans on proteins, and these α-L-fucosidases clearly play important roles in biology. GH29 enzymes work via a retaining exo-action mechanism, and some can catalyze transfucosylation. There is no formal subfamily division of GH29 α-L-fucosidases, but they are nonetheless divided into two subfamilies: GH29A having a range of substrate specificities and GH29B having narrower substrate specificity. However, the sequence traits that determine the substrate specificity and transglycosylation ability of GH29 enzymes are not well characterized. Here, we present a new functional map of family GH29 members based on peptide-motif clustering via CUPP (conserved unique peptide patterns) and compare the substrate specificity and transglycosylation activity of 21 representative α-L-fucosidases across the 53 CUPP groups identified. The 21 enzymes exhibited different enzymatic rates on 8 test substrates, CNP-Fuc, 2’FL, 3FL, Lewisa, Lewisx, Fuc-α1,6-GlcNAc, Fuc-α1,3-GlcNAc, and Fuc-α1,4-GlcNAc. Certain CUPP groups clearly harbored a particular type of enzymes, e.g. the majority of the enzymes having activity on Lewisa or Lewisx categorized in the same CUPP clusters. In general, CUPP was useful for resolving GH29 into functional diversity subgroups when considering hydrolytic activity. In contrast, the transglycosylation capacity of GH29 α-L-fucosidases was distributed across a range of CUPP groups. Transglycosylation thus appears to be a common trait among these enzymes and not readily predicted from sequence comparison.
Dissection of a Down syndrome-associated trisomy to separate the gene dosage-dependent and -independent effects of an extra chromosome
Authors: Xing Zhuo , Li Yichen , Cortes-Gomez Eduardo , Jiang Xiaoling , Gao Shuang , Pao Annie , Shan Jidong , Song Yinghui , Perez Amanda , Yu Tao , Highsmith Max R , Boadu Frimpong , Conroy Jeffrey M , Singh Prashant K , Bakin Andrei V , Cheng Jianlin , Duan Zhijun , Wang Jianmin , Liu Song , Tycko Benjamin , Yu Y Eugene 
Published: April 2023

AbstractAs an aneuploidy, trisomy is associated with mammalian embryonic and postnatal abnormalities. Understanding the underlying mechanisms involved in mutant phenotypes is broadly important and may lead to new strategies to treat clinical manifestations in individuals with trisomies, such as trisomy 21 [Down syndrome (DS)]. Although increased gene dosage effects because of a trisomy may account for the mutant phenotypes, there is also the possibility that phenotypic consequences of a trisomy can arise because of the presence of a freely segregating extra chromosome with its own centromere, i.e. a ‘free trisomy’ independent of gene dosage effects. Presently, there are no reports of attempts to functionally separate these two types of effects in mammals. To fill this gap, here we describe a strategy that employed two new mouse models of DS, Ts65Dn;Df(17)2Yey/+ and Dp(16)1Yey/Df(16)8Yey. Both models carry triplications of the same 103 human chromosome 21 gene orthologs; however, only Ts65Dn;Df(17)2Yey/+ mice carry a free trisomy. Comparison of these models revealed the gene dosage-independent impacts of an extra chromosome at the phenotypic and molecular levels for the first time. They are reflected by impairments of Ts65Dn;Df(17)2Yey/+ males in T-maze tests when compared with Dp(16)1Yey/Df(16)8Yey males. Results from the transcriptomic analysis suggest the extra chromosome plays a major role in trisomy-associated expression alterations of disomic genes beyond gene dosage effects. This model system can now be used to deepen our mechanistic understanding of this common human aneuploidy and obtain new insights into the effects of free trisomies in other human diseases such as cancers.
Providing more effective kangaroo care during the COVID-19 pandemic: A quality improvement project in a Lebanese neonatal intensive care unit
Authors: Abi Farraj Farida , Masri Saadieh , Maalouf Faouzi I , Charafeddine Lama 
Published: April 2023

AbstractBackgroundKangaroo care has become the standard in caring for preterm infants; however, its application still faces many barriers due to insufficient staff or parental education or participation and most recently visitation restrictions after the COVID-19 pandemic.MethodsThis quality improvement project (QIP) took place in a tertiary center’s neonatal intensive care unit in Lebanon from September 2018 to March 2021. It aimed to increase kangaroo care practice and maternal milk use among admitted infants. Nurses and parents received education and hands-on training about kangaroo care and skin-to-skin contact. Nurses’ and parents’ knowledge, attitude, and behavior toward kangaroo care were evaluated pre- and post-intervention. Balancing measures included hypothermia, central line infection, catheter dislodgement, and babies being dropped. COVID-19 visitation restrictions during QIP cycles were documented.ResultsA total of 143 infants received kangaroo care during the project period and 105 (73%) were ≤34 weeks of gestation. By the end of the QIP, kangaroo care practice increased from 2.5 to 7 h per infant stay (from a median of 45 min per session to 60 min per session). Infants receiving more than five kangaroo care sessions had higher maternal milk use (71.3% vs. 52.8%; P = 0.002) and growth velocity (12.1 vs. 2.0 g/kg/day; P &lt; 0.001). Parents’ perceived behavior and frequency of performing kangaroo care–related tasks improved significantly (P &lt; 0.005) compared to before QIP. Thirty-seven of 44 participating nurses reported more perceived behavior transferring ventilated babies (P = 0.049).ConclusionThis QIP successfully increased kangaroo care practice and maternal milk use in a resource-limited environment, despite COVID-19 restrictions. More work is needed to ensure sustainability and replicability.