bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation Testing of a New Crutch Tip Biofeedback Device for Prescribed Lower Extremity Weight-Bearing
Authors: Brueilly Kevin E. , Feller Amanda M. , Ahearn Jonathan M. , Goodwin Jonathan S. 
Published: August 2023

Introduction:
Modified weight-bearing recommendations are commonly prescribed after surgical intervention for injuries to the lower extremity to reduce the risk of nonunion and delayed healing associated with load bearing through the injured limb and to combat the deleterious effects of immobility. The physical therapist (PT) in the acute care setting is likely to instruct patients after lower extremity injury in weight-bearing-restricted ambulation. A new device is now available for use in the training of weight-bearing status. The study examines whether the ComeBack Mobility crutch tip reporting weight-bearing on the lower extremity is a reliable and valid tool in determining force when compared with the gold standard force plate measurement of lower extremity weight-bearing.

Review of Literature:
Previous studies have shown that patients are often not able to adequately learn or adhere to restrictive weight-bearing modifications. This may be due to an inability to provide immediate and ongoing feedback on weight-bearing. The new ComeBack Mobility crutch tip system is now available for the acute care PT to use in instruction and for patients to receive real-time feedback throughout their rehabilitation process.

Subjects:
A sample of convenience of 6 able-bodied PTs was used.

Methods:
Each subject performed 30 trials of axillary crutch-assisted weight-bearing ambulation using the new device. The weight-bearing reported by the device was compared with the weight-bearing measured through force plates via correlations, t tests, and Bland-Altman plot.

Results:
The new device demonstrated moderate-good reliability in the measurement of non-weight-bearing and 50% partial weight-bearing in trials completed.

Discussion and Conclusion:
The ComeBack Mobility crutch tip system could be useful and should be considered for clinical use as a reliable and valid tool in providing auditory feedback for compliance to a prescribed weight-bearing protocol. The system could be useful in the training of patients in the first use of crutches such as prior to discharge from an acute care hospital. Further research is needed with clinical populations as well as with varied weight-bearing protocols.
The Effect of a Biofeedback-Based Integrated Program on Improving Orthostatic Hypotension in Community-Dwelling Older Adults
Authors: Han Jeonghwa , Park Jooyeon , Kang Hyunwook , Lee Hyung , Kim Nahyun 
Published: August 2023

Background
Orthostatic hypotension (OH) is prevalent among community-dwelling older adults and is associated with multiple negative health outcomes. Older adults are susceptible to developing OH because aging alters autonomic nervous system function. Biofeedback is a noninvasive, nonpharmacological intervention that can modulate autonomic nervous system dysfunction in older adults.

Objectives
Our aim in this study was to examine the effect of a biofeedback-based integrated program on community-dwelling older adults with OH.

Methods
We conducted a controlled pilot study. Community-dwelling older adults 65 years or older who had nonneurogenic OH were eligible. Data from 51 participants, comprising 27 in the intervention group and 24 in the control group, were analyzed. Weekly biofeedback-based integrated program consisting of biofeedback training along with group education about behavioral modification, physical activities, and telephone counseling was provided for 12 weeks. Orthostatic hypotension was evaluated by measuring the drop in systolic and diastolic blood pressure after postural changes. Autonomic nervous system function was measured using heart rate variability.

Results
Among the indicators of heart rate variability, total power (P = .037) and low frequency (P = .017) increased significantly, suggesting that autonomic function improved. Severity of orthostatic symptoms (P < .001) and drops in systolic (P = .003) and diastolic (P = .012) blood pressure after postural changes decreased significantly in the intervention group.

Conclusion
Biofeedback-based integrated program was effective in improving autonomic nervous system function and alleviated OH. Therefore, biofeedback-based integrated program should be tested in a larger randomized controlled study with long-term follow-up.
Improving access to prosthetic limbs in Germany: An explorative review
Authors: Baumann Martina F. , Maria Maia 
Published: August 2023

Background:
Meeting the needs of users when it comes to accessing prosthetic limbs is an important factor in the acceptance and use of a prosthesis; the cost of such prosthetics also constitutes a potential financial challenge.

Objectives:
The aim of this study was to investigate potential hurdles to accessing limb prosthetics in the German health care system, including organizational, social, economic, and regulatory issues, and to provide food for thought about ethical implications.

Methods:
Sixteen German users of limb prosthetics with upper-limb and/or lower-limb amputation were recruited by means of purposive sampling. Semistructured interviews were performed, with the guiding question being as follows: “What were your experiences with the German prosthetic care and reimbursement system?” Ten stakeholders (insurance representatives, prosthetic technicians, medical service representatives, a law expert, and a lawyer) were asked about the issues they encounter in their work related to prosthetic care and reimbursement, and about ways to ameliorate these issues. A qualitative content analysis method was used to analyze the data.

Results:
Half of the interviewed service users experienced hurdles to gaining a suitable prosthetic device, such as waiting times and pressure to negotiate their need for a certain prosthesis. Some of the views expressed about the issues relating to prosthetic reimbursement in Germany were common to all stakeholders, whereas some conflicted with the views of others.

Conclusions:
Equitable access to prostheses and the efficient distribution of prosthetic innovations could be improved by organizational and regulatory measures. Furthermore, a user-centered design of prostheses, a health technology assessment, monitoring of prosthetic care pathways, and a societal discussion about rationing in health care should be considered as parts of a broader approach to tackle this issue.
Barriers and facilitators of receiving prosthetic services in people with lower limb amputation during Covid-19 pandemic: A qualitative study in a developing country
Authors: Nabizadeh Samira , Jalali Maryam , Kamali Mohammad , Shahabi Saeed , Babaee Taher 
Published: August 2023

Purpose:
This study investigated the barriers, challenges, and facilitators in receiving prosthetic services in people with lower limb amputation during Covid-19 pandemic.

Materials and methods:
In this qualitative study, we conducted in-depth semistructured face-to-face interviews with individuals with lower limb amputation who needed prosthetic services during Covid-19 pandemic. We discussed about challenges and barriers that the participants had encountered during this period. The collected data were analyzed using a conceptual framework.

Results:
Six broad themes of availability, accessibility, acceptability, affordability, accommodation, and awareness emerged from the analysis. Staff shortage, lack of remote services, accommodation, insurance and governmental support, and cost of services were the main concerns. Moreover, long waiting lists, postponed referrals, noncompliance with Covid-19 protocols and preventive measures, and lockdowns were the other important concerns that were noted. In addition, unsatisfactory services in hometown, distance to capital, transportation permit in lockdowns, traffic jam, and public transport problems were other reasons of service not being accessible. The positive aspects were the facilitators such as having home visits, social work and charity, and using private drive services.

Conclusion:
It seems that after the beginning of Covid-19 pandemic, people with lower limb amputation have faced more drastic challenges in accessing and receiving prosthetic services than in the prepandemic situations.
Redefining Hemodynamic Imaging in Stroke: Perfusion Parameter Map Generation from TOF-MRA using Artificial Intelligence
Authors: Kossen Tabea , Madai Vince I , Lohrke Felix , Aydin Orhun Utku , Behland Jonas , Hilbert Adam , Mutke Matthias A , Bendszus Martin , Sobesky Jan , Frey Dietmar 
Published: August 2023

AbstractBackgroundPerfusion assessment in cerebrovascular disease is essential for evaluating cerebral hemodynamics and guides many current treatment decisions. Dynamic susceptibility contrast (DSC) magnetic resonance imaging (MRI) is of great utility to generate perfusion parameter maps, but its reliance on a contrast agent with associated health risks and technical challenges limit its usability. We hypothesized that native Time-of-flight magnetic resonance angiography (TOF-MRA) can be used to generate perfusion parameter maps with an artificial intelligence (AI) method, called generative adversarial network (GAN), offering a contrast-free alternative to DSC-MRI.MethodsWe propose an adapted 3D pix2pix GAN that generates common perfusion maps from TOF-MRA images (CBF, CBV, MTT, Tmax). The models are trained on two datasets consisting of 272 patients with acute stroke and steno-occlusive disease. The performance was evaluated by the structural similarity index measure (SSIM), for the acute dataset we calculated the Dice coefficient for lesions with a time-to-maximum (Tmax) >6s.FindingsOur GAN model showed high visual overlap and high performance for all perfusion maps on both the acute stroke dataset (mean SSIM 0.88-092) and data including steno-occlusive disease patients (mean SSIM 0.83–0.98). For lesions of Tmax>6, the median Dice coefficient was 0.49.InterpretationOur study shows that our AI model can accurately generate perfusion parameter maps from TOF-MRA images, paving the way for clinical utility. We present a non-invasive alternative to contrast agent-based imaging for the assessment of cerebral hemodynamics in patients with cerebrovascular disease. Leveraging TOF-MRA data for the generation of perfusion maps represents a groundbreaking approach in cerebrovascular disease imaging. This method could greatly impact the stratification of patients with cerebrovascular diseases by providing an alternative to contrast agent-based perfusion assessment.FundingThis work has received funding from the European Commission (Horizon2020 grant: PRECISE4Q No. 777107, coordinator: DF) and the German Federal Ministry of Education and Research (Go-Bio grant: PREDICTioN2020 No. 031B0154 lead: DF).
Extended toolboxes enable efficient biosynthesis of valuable chemicals directly from CO2in fast-growingSynechococcussp. PCC 11901
Authors: Zhang Tong , Li Shubin , Chen Lei , Sun Tao , Zhang Weiwen 
Published: August 2023

AbstractCO2recycle is crucial to the global carbon neutrality. Though cyanobacteria are known to be photoautotrophic cell factories capable of converting CO2into valuable chemicals, their slower growth rate and lower biomass accumulation compared to those of the heterotrophic organisms significantly restrict their application at commercial scale. The newly discovered marine cyanobacterium, Synechococcus sp. PCC 11901 (hereafter PCC 11901) offers several advantages like rapid growth, high biomass and high salinity tolerance, and could become a new generation of cyanobacterial chassis. To promote its application, in this study we developed genetic toolboxes applicable to PCC11901. First, a cobalamin (VB12)-independent chassis was constructed, allowing for its cheaper cultivation. Second, genome copy numbers and transformation methods were respectively measured and optimized. The 14 neutral sites were identified and characterized within the genome PCC 11901, providing locations for genetic integration of exogenous cassettes. Subsequently, libraries were developed, reaching an expression range of approximately 800 folds for constitutive promoters and an induction fold of up to approximately 400 for inducible promotor, respectively. As a proof of concept of its utilization, we engineered the synthetic pathways of glucosylglycerol (GG) into PCC 11901 using the established toolboxes, yielding 590.41 ± 21.48 mg/L for GG production. Notably, we found the cobalamin-independent PCC 11901 chassis exhibited superior self-sedimentation ability compared to the wild-type chassis. Our work here made it possible to develop the fast-growing PCC 11901 as efficient carbon-neutral cell factory in the future.
YY1 Binding to Regulatory Elements that Lack Enhancer Activity Promotes Locus Folding and Gene Activation
Authors: Gao Zeqian , Wang Miao , Smith Alastair , Boyes Joan 
Published: August 2023

AbstractEnhancers activate their cognate promoters over huge distances but how enhancer/promoter interactions become established is not completely understood. There is strong evidence that cohesin-mediated loop extrusion is involved but this does not appear to be a universal mechanism. Here, we identify an element within the mouse immunoglobulin lambda (Igλ) light chain locus, HSCλ1, that has characteristics of active regulatory elements but lacks intrinsic enhancer or promoter activity. Remarkably, knock-out of the YY1 binding site from HSCλ1 reduces Igλ transcription significantly and disrupts enhancer/promoter interactions, even though these elements are >10 kb from HSCλ1. Genome-wide analyses of mouse embryonic stem cells identified 3503 similar YY1-bound, putative genome organizing elements that lie within CTCF/cohesin loop boundaries but that lack intrinsic enhancer activity. We suggest that such elements play a fundamental role in locus folding and in facilitating enhancer/promoter interactions.HighlightsHow long-range enhancer-promoter interactions are established is not fully understood An element in the lambda light chain locus, HSCλ1, lacks intrinsic enhancer activity Removal of YY1 binding from HSCλ1 disrupts neighbouring enhancer/promoter contacts Genome-wide analyses detect similar elements that lack enhancer or promoter activity We propose these elements aid locus folding and nearby enhancer-promoter interactions
The role of employee environmental commitment in the green HRM practices, turnover intentions and environmental sustainability nexus
Authors: Suleman Abdul-Razak , Amponsah-Tawiah Kwesi , Ametorwo Aaron Makafui 
Published: August 2023

PurposeThe study sought to examine the mediating effect of employee environmental commitment (EEC) in the relationships between green human resource management practices (Green HRMPs) and environmental sustainability (ES) and employee turnover intentions (ETI).Design/methodology/approachUsing a cross-sectional survey approach, data was sourced from 296 respondents from five manufacturing firms listed on the Ghana Stock Exchange. Data was analysed using descriptive and inferential statistics with the help of Statistical Package for Social Sciences (SPSS) and SmartPLS.FindingsResults showed that EEC had a partial mediation effect on the relationship between Green HRMPs and ES at the organisational level but fully mediated the relationship between Green HRMPs and ETI at the individual level.Practical implicationsIt is evident from the findings that Green HRMPs improve ES and employee stability. Therefore, practitioners and educational institutions should consider integrating environmental concerns into their functional processes and operations. In addition, governments as policymakers should formulate and strengthen the enforcement of already-existing environmental management and sustainability policies that promote the integration of green practices and initiatives in both private and public entities.Originality/valueLittle is known about Green HRM and its effect on organisational and individual-level outcomes, especially in Sub-Saharan Africa. This study presents empirical evidence from an emerging economy.
The influence of online host–guest interaction on listing popularity in peer-to-peer accommodation: the role of listing price and reputation
Authors: Liu Fuzhen , Lai Kee-hung , He Chaocheng 
Published: August 2023

PurposeTo promote the success of peer-to-peer accommodation, this study examines the effects of online host–guest interaction as well as the interaction's boundary conditions of listing price and reputation on listing popularity.Design/methodology/approachUsing 330,686 data collected from Airbnb in the United States of America, the authors provide empirical evidence to answer whether social-oriented self-presentation and response rate influence listing popularity from the perspective of social exchange theory (SET). In addition, the authors investigate how these two kinds of online host–guest interactions work with listing price and reputation to influence listing popularity.FindingsThe results reveal the positive association between online host–guest interaction and listing popularity. Notably, the authors find that listing price strengthens but listing reputation weakens the positive effects of online host–guest interactions on listing popularity in peer-to-peer accommodation.Originality/valueThis study is the first attempt to adopt SET to explain the importance of online host–guest interactions in influencing listing popularity as well as examine the moderating role of listing price and reputation on the above relationship.
Progress in network orchestration research and future prospects: a bibliometric analysis
Authors: Wang Xiaorui , He Di 
Published: August 2023

PurposeNetwork orchestration has received widespread attention from scholars engaged in network-relationship governance research. This study aims to explore progress in network orchestration research and future prospects.Design/methodology/approachIn this study, 105 articles related to network orchestration from the Web of Science Core Collection Database for the period 2006–2022 are collected as sample data, and bibliometric relationship maps of network orchestration research are visually presented using the CiteSpace software.FindingsFirst, this study presents distributions of articles, methods, journals, authors, institutions and countries/regions in network orchestration research. Second, based on a reference co-citation analysis, three articles are identified as the key literature in network orchestration research. Based on a keyword co-occurrence analysis, the hotspots are found to include studies related to overall network and individual levels. A cluster analysis reveals six themes: innovation ecosystem, sustainable development, product development, sourcing, hub firm and innovation network. From a burst detection analysis, three frontiers emerge: organisation, collaboration and governance. Finally, some future research directions are proposed and important issues are raised.Originality/valueThis study is the first to reveal the progress of network orchestration research using quantitative bibliometric methods, and it provides insights to scholars and offers practitioners with a better understanding of network orchestration.
TLR2 mediates autophagy through ERK signaling pathway in Chlamydia psittaci CPSIT_p7 protein‐stimulated RAW264.7 cells
Authors: Luo Ying , Sun Zhenjie , Chen Qian , Xiao Jian , Yan XiaoLiang , Li Yumeng , Wu Yimou 
Published: August 2023

AbstractChlamydia psittaci is a zoonotic pathogen found in birds and humans. Macrophages, major components of the innate immune system, can resist chlamydial infections and trigger adaptive immune responses. However, the molecular mechanisms underlying the action of macrophages against C. psittaci infection are not well understood. This study investigated the roles and mechanisms of plasmid‐encoded protein CPSIT_p7 of C. psittaci in regulating autophagy in RAW264.7 cells. The results demonstrated that stimulation of RAW264.7 with C. psittaci plasmid protein CPSIT_p7 induced the expressions of the autophagy signaling primary regulators LC3 and Beclin1, which could also significantly induce the phosphorylation levels of ERK, JNK, p38, and Akt. Next, siRNA knockdown of TLR2 resulted in significant downregulation of CPSIT_p7‐triggered autophagy in RAW264.7 cells. Moreover, the extracellular regulated protein kinase (ERK) inhibitor PD98059 markedly reduced autophagy in CPSIT_p7‐stimulated macrophages. In summary, these results indicated that TLR2 plays an essential role in the induction of autophagy through the ERK signaling pathway in CPSIT_p7‐stimulated RAW264.7 cells.
Effect of potato starch coating containing selected amino acids to prevent the formation of PhIP in pan‐fried chicken breast
Authors: Farshi Parastou , Hashempour‐Baltork Fatane , Amamcharla Jayendra , Smith J. Scott 
Published: August 2023

AbstractThe effects of potato starch (PS) coating containing amino acids (AAs) on the formation of 2‐amino‐1‐methyl‐6‐phenylimidazo [4,5‐b] pyridine (PhIP) in chicken breasts were evaluated. PhIP is classified as a Group 2B carcinogen by the International Agency for Research on Cancer (IARC). The 5% (w/w) gelatinized PS coating solution was incorporated with tryptophan (Trp) or lysine (Lys) at 0.25%, 0.5%, and 0.75% (w/w of the coating solution) concentrations. Chicken breast cuts with the same dimensions (5 × 2 × 1.5 cm) were dipped in the PS coating solution for 15 min before frying. After frying the chicken at 195°C for 7.5 min on each side, PhIP levels, color, cooking loss, tenderness, and texture profile assay were evaluated. The average PhIP concentration was decreased from 92.62 ng/g for the control chicken breast without coating to 6.30 ng/g (0.25% Lys), 6.76 ng/g (0.5% Lys), and 11.98 ng/g (0.75% Lys), accounting for an 89%–92% reduction in PhIP levels compared to the controls. However, dipping in Trp‐containing PS coating had a significantly lower (p < 0.05) PhIP reduction effect (34%–67%). There was no significant difference in cooking loss percentage, tenderness, texture profile parameters, and color parameters of PS‐coated chicken. Triangle test results showed that consumers did not detect a significant difference in the PS‐coated chicken breasts (p < 0.001). Overall, this study suggests that the application of PS‐based coatings incorporated with AAs on chicken breast reduces the PhIP formation.
Acute myeloid leukaemia in Afghanistan: Understanding an unfamiliar landscape
Authors: Hills Robert K. 
Published: August 2023

The paper by Noor et al. in this issue provides important and interesting data on the incidence and outcomes of patients with acute myeloid leukaemia in Afghanistan. The age of patients is much lower than we are used to seeing in the West, reflecting in part the particular demographics of the country; these data provide an important first step to identifying areas for improvement.Commentary on: Noor et al. Demographic and clinical characteristics of acute myeloid leukaemia diagnosed and treated at tertiary level in Afghanistan. Br J Haematol 2023;203:404–410.