bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anatomical damage caused by Bacillus thuringiensis variety israelensis in yellow fever mosquito Aedes aegypti (L.) larvae revealed by micro-computed tomography
Authors: Alba-Tercedor Javier , Vilchez Susana 
Published: May 2023

AbstractWith micro-computed tomography techniques, using the single-distance phase-retrieval algorithm phase contrast, we reconstructed enhanced rendered images of soft tissues of Aedes aeqypti fourth instar larvae after Bti treatment. In contrast to previous publications based on conventional microscopy, either optical or electron microscopy, which were limited to partial studies, mostly in the form of histological sections, here we show for the first time the effects of Bti on the complete internal anatomy of an insect. Using 3D rendered images it was possible to study the effect of the bacterium in tissues and organs, not only in sections but also as a whole. We compared the anatomy of healthy larvae with the changes undergone in larvae after being exposed to Bti (for 30 min, 1 h and 6 h) and observed the progressive damage that Bti produce. Damage to the midgut epithelia was confirmed, with progressive swelling of the enterocytes, thickening epithelia, increase of the vacuolar spaces and finally cell lysis, producing openings in the midgut walls. Simultaneously, the larvae altered their motility, making it difficult for them to rise to the surface and position the respiratory siphon properly to break surface tension and breathe. Internally, osmotic shock phenomena were observed, resulting in a deformation of the cross-section shape, producing the appearance of a wide internal space between the cuticle and the internal structures and a progressive collapse of the tracheal trunks. Taken together, these results indicate the death of the larvae, not by starvation as a consequence of the destruction of the epithelia of the digestive tract as previously stated, but due to a synergic catastrophic multifactor process in addition to asphyxia due to a lack of adequate gas exchange.
Experimental study on impermeability of Loess liner mixed with bentonite-HDTMA
Authors: Ming Zhang , Dongke Hu , Shaoyu Pan , Guozhou Chen 
Published: May 2023

AbstractPermeability tests are performed by using the flexible wall permeameter to study the influence of bentonite-HDTMA (hexadecyl trimethyl ammonium bromide) on the permeability performance of Loess as a lining material in the solid waste landfill. Results show, the impermeability of compacted Loess in the middle and lower reaches of the Yellow River in China does not meet the standard requirement as the landfill liner. The permeability of Loess mixed with more than 10% bentonite ratio is less than 1.0 × 10−7 cm·s−1. The permeability of Loess increases slightly after mixing a small amount of HDTMA. The impermeability of Loess mixed with some bentonite-HDTMA ratio still meet the standard requirement. The HDTMA may destroy the soil aggregate structure and increase the soil permeate channel. The SEMs show that the bentonite clay particle can fill the pores between Loess coarse particles and improve the impermeability performance of the material. The digital photos show that HDTMA can effectively resist the development of soil macro-cracks induced by wetting–drying cycles, better for liner to maintain good impermeability. On this basis, the relationship between the hydraulic conductivity of bentonite-HDTMA modified Loess and dry density is constructed. From this study, Loess can be used as a lining material for landfills, when mixed with bentonite/HDTMA at ratios of 10%/0% or 14%/2%.
Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae
Authors: Fayet Maurine , Prybylski Nastasia , Collin Marie-Laure , Peyretaillade Eric , Wawrzyniak Ivan , Belkorchia Abdel , Akossi Reginald Florian , Diogon Marie , El Alaoui Hicham , Polonais Valérie , Delbac Frédéric 
Published: May 2023

AbstractMicrosporidia are obligate intracellular parasites able to infect a wide range of hosts from invertebrates to vertebrates. The success of their invasion process is based on an original organelle, the polar tube, which is suddenly extruded from the spore to inoculate the sporoplasm into the host cytoplasm. The polar tube is mainly composed of proteins named polar tube proteins (PTPs). A comparative analysis allowed us to identify genes coding for 5 PTPs (PTP1 to PTP5) in the genome of the microsporidian Anncaliia algerae. While PTP1 and PTP2 are found on the whole polar tube, PTP3 is present in a large part of the extruded polar tube except at its end-terminal part. On the contrary, PTP4 is specifically detected at the end-terminal part of the polar tube. To complete PTPs repertoire, sequential sporal protein extractions were done with high concentration of reducing agents. In addition, a method to purify polar tubes was developed. Mass spectrometry analysis conducted on both samples led to the identification of a PTP3-like protein (PTP3b), and a new PTP (PTP7) only found at the extremity of the polar tube. The specific localization of PTPs asks the question of their roles in cell invasion processes used by A. algerae.
Failure analysis and prediction of roof instability in end face under repeated mining using early warning system
Authors: Li Fei , Kong Dezhong , Li Qiang , Shang Yuqi , Cheng Zhanbo , He Liuquan 
Published: May 2023

AbstractThe overlying strata of the lower coal seam is easy to be collapsed causing the roof caving accident at the end face of the mining working face under repeated mining in close-distance coal seams. In order to predict the roof instability of the end face, the mechanical model of the granular arch structure is established in this study to further analyze its main influencing factors. The results show that the mining height of the working face, the advancing speed, the distance of coal seams, the tip-to-face distance, the strength of the surrounding rock and the support setting the load of the support are the main influencing factors on the roof caving of the end face. Subsequently, the prediction model of roof instability in the end face under repeated mining is constructed through the radial basis function neural network (RBFNN) and the above main influencing factors are regarded as input layer indexes. Meanwhile, the roof subsidence, coal wall deformation and support load are determined as the output layer indexes. The predicted results are closer to the results of sample tests. Finally, the early warning system, including monitoring and early warning, data query, emergency management, user management, and system settings, is designed to monitor roof conditions of the end face and timely warn the roof accidents. The field application proves that the system has good practical value, which is of great significance to intelligent prediction of coal mine stope disaster and prevent the end face roof disaster under repeated mining and. This will promote the safe and efficient construction of coal mine production.
Cracks propagation characteristics of double-hole delay blasting in soft-hard composite rock mass
Authors: Cui Jianbin , Xie Liangfu , Qin Yongjun , Liu Xuejun , Wang Jianhu , Qian Jiangu 
Published: May 2023

AbstractBy researching the distance between blasthole and interface of soft-hard rock strata, as well as the time of delay detonation, blasting effect of the rock mass will be more controllable. Firstly, validity of numerical method was authenticated from three angles: blasting coupled stress field, ratio of crushing zone radius to blasthole radius, and crack network state. Under the condition of soft-hard rock strata, numerical model of double-hole blasting was established by using PFC2D. Then delay blasting experiments were carried out under different relative positions of blasthole and interface. Ultimately, results were analyzed from three perspectives: crack network, crack quantity and rock fragment. Results show that: (1) When detonated in hard rock, if between interface and blasthole distance is greater than twice crushing zone radius, the closer blasthole is to the interface, the more obvious the “hook” phenomenon between the two blastholes is. With increasing delayed initiation time, “hook” phenomenon will weaken or even disappear. (2) Based on the crack information initiated in hard rock, the law of crack number varying with thickness of hard rock and delay time is obtained. (3) For initiation in hard rock, crack extension range is large, but less fragments are formed. The law is opposite to that initiation in structural plane and soft rock. Fragmentation area increases exponentially with increasing soft rock thickness, and exponential function is obtained.
Surveillance of prognostic risk factors in patients with SCCB using artificial intelligence: a retrospective study
Authors: Zhanghuang Chenghao , Zhang Zhaoxia , Wang Jinkui , Yao Zhigang , Ji Fengming , Wu Chengchuang , Ma Jing , Yang Zhen , Xie Yucheng , Tang Haoyu , Yan Bing 
Published: May 2023

AbstractSmall cell carcinoma of the bladder (SCCB) is a rare urological tumor. The prognosis of SCCB is abysmal. Therefore, this study aimed to construct nomograms that predict overall survival (OS) and cancer-specific survival (CSS) in SCCB patients. Information on patients diagnosed with SCCB during 2004–2018 was obtained from the Surveillance, Epidemiology, and End Results (SEER) database. Univariate and multivariate Cox regression models analyzed Independent risk factors affecting patients' OS and CSS. Nomograms predicting the OS and CSS were constructed based on the multivariate Cox regression model results. The calibration curve verified the accuracy and reliability of the nomograms, the concordance index (C-index), and the area under the curve (AUC). Decision curve analysis (DCA) assessed the potential clinical value. 975 patients were included in the training set (N = 687) and the validation set (N = 288). Multivariate COX regression models showed that age, marital status, AJCC stage, T stage, M stage, surgical approach, chemotherapy, tumor size, and lung metastasis were independent risk factors affecting the patients' OS. However, distant lymph node metastasis instead AJCC stage is the independent risk factor affecting the CSS in the patients. We successfully constructed nomograms that predict the OS and CSS for SCCB patients. The C index of the training set and the validation set of the OS were 0.747 (95% CI 0.725–0.769) and 0.765 (95% CI 0.736–0.794), respectively. The C index of the CSS were 0.749 (95% CI 0.710–0.773) and 0.786 (95% CI 0.755–0.817), respectively, indicating that the predictive models of the nomograms have excellent discriminative power. The calibration curve and the AUC also show good accuracy and discrimination of the nomograms. To sum up, We established nomograms to predict the OS and CSS of SCCB patients. The nomograms have undergone internal cross-validation and show good accuracy and reliability. The DCA shows that the nomograms have an excellent clinical value that can help doctors make clinical-assisted decision-making.
Privacy and the acceptance of centralized digital currencies in the U.S., India and Germany
Authors: Mehlkop Guido , Neumann Robert , von Hermanni Hagen 
Published: May 2023

AbstractNational governments around the world increasingly acknowledge the possibility of introducing new digital forms of money and implementing policies that trigger their adoption. Knowledge about the acceptance of such measures, however, is rather limited. Next to the regulatory uncertainty about the impact of a Central Bank Digital Currency (CBDC) on competition, on financial stability and questions on the integrity and technical implementations of a CBDC, recent announcements of the joint venture of e.g. the European Central Bank with a large and globally operating private company emphatically raise questions about data privacy. Therefore, we report results of a survey experiment conducted in the United States of America, Germany and India to investigate the acceptance of an app-based monthly digital payment similar to a Universal Basic Income and investigate its adoption across income levels. Controlling for privacy features and short-term vs. long-term incentives to adopt the digital payment app, we find strong reservations with regard to the involvement of multinational tech companies in establishing new digital mediums of exchange, while also finding contextual differences in acceptance levels between the studied populations.
Experimental study on damage characteristics of Beishan granite under single loading and multiple loading with AE techniques
Authors: Wang Zihui , Zhou Guanglei , Ge Xinbo 
Published: May 2023

AbstractAn insight into the damage characteristics of host rock is important for permanent disposal of high-level radioactive waste (HLW). Single loading and multiple loading tests for Beishan granite were carried out. The acoustic emission (AE) monitoring system was utilized simultaneously to measure the damage evolution in the specimens. The results show that, during single loading the AE signal has been maintained at a very high level after dilatancy. But a decreasing trend of AE signals can be observed when the axial stress approaches the peak stress. While during multiple loading, AE signals are mainly generated in the loading period in the elastic stage. However, in the failure and residual stages, AE signals generated in the unloading period are considerable. Most of the AE waveforms have a dominant frequency of less than 200 kHz and amplitude of less than 80 dB, and three frequency bands are obtained. In addition, the amplitude increases with the increase of loading stress. Furthermore, the obtained AE b-values indicate that the relative proportion of micro- to macro-cracks increases first and then decreases with increasing confining pressure. And the AE a-values and b-values confirms that micro cracks develop rapidly in the damage stage and lead to the formation of macro cracks in the residual stage for the specimens experienced multiple loading.
Plant viruses induce plant volatiles that are detected by aphid parasitoids
Authors: Milonas Panagiotis G. , Anastasaki Eirini , Psoma Aikaterini , Partsinevelos Georgios , Fragkopoulos Georgios N. , Kektsidou Oxana , Vassilakos Nikon , Kapranas Apostolos 
Published: May 2023

AbstractAphis gossypii (Sternorrhyncha: Aphididae) aphids are vectors of important plant viruses among which cucumber mosaic virus (CMV) and potato virus Y (PVY). Virus-infected plants attract aphid vectors and affect their behavior and growth performance either positively or negatively depending on mode of transmission. Viruses cause changes in the composition and the amount of volatile organic compounds (VOCs) released by the plant that attract aphids. The aphid parasitoid Aphidius colemani (Hymenoptera: Aphelinidae) has been shown to have higher parasitism and survival rates on aphids fed on virus-infected than aphids fed on non-infected plants. We hypothesized that parasitoids distinguish virus-infected plants and are attracted to them regardless of the presence of their aphid hosts. Herein, we examined the attraction of the A. colemani parasitoid to infected pepper plants with each of CMV or PVY without the presence of aphids. The dynamic headspace technique was used to collect VOCs from non-infected and CMV or PVY-infected pepper plants. Identification was performed with gas chromatography-mass spectrometry (GC–MS). The response of the parasitoids on virus-infected vs non-infected pepper plants was tested by Y-tube olfactometer assays. The results revealed that parasitoids displayed a preference to CMV and PVY infected plants compared to those that were not infected.
A longitudinal Q-study to assess changes in students’ perceptions at the time of pandemic
Authors: Akhtar-Danesh Noori , Brewer-Deluce Danielle , Saini Jessica , Wojkowski Sarah , Bayer Ilana , Saraco Anthony N. , Pitt Courtney , Wainman Bruce 
Published: May 2023

AbstractThe COVID-19 pandemic forced many universities and colleges to rapidly adopt online course delivery. As with any new foray, realizing the optimal aspects of a course to change became incredibly important for course instructors. In this study, we used a particularly sensitive method, i.e. Q-methodology, to evaluate changes based on students’ perceptions from fall 2020 to winter 2021. Q-methodology is commonly used to uncover shared values, opinions, and preferences. Using Q-methodology, students participating in both semesters of an undergraduate anatomy and physiology course were surveyed in fall 2020 and winter 2021. The Q-sample included 44 statements. Data from fall 2020 were treated as the baseline and changes in students’ perceptions from 2020 to 2021 were assessed. In total, 31 students completed both fall 2020 and winter 2021 course evaluations. Three salient factors emerged from the fall 2020 evaluation: Overtaxed students, Solo Achievers, and In-Person Learners. At the baseline, students were concerned mostly about the delivery of the course, then the winter 2021 evaluation showed how they were adjusting to online learning. The longitudinal Q-study proved to be robust in identifying changes in perceptions. These granular findings indicate how students might differ in viewing and evaluating online courses. This methodology can be used in redesigning and restructuring different components of an online course in higher education settings.
Diagnosis of sudden cardiac arrest using principal component analysis in automated external defibrillators
Authors: Pham Van-Su , Nguyen Anh , Dang Hoai Bac , Le Hai-Chau , Nguyen Minh Tuan 
Published: May 2023

AbstractSudden cardiac arrest (SCA) consisting of ventricular fibrillation and ventricular tachycardia considered as shockable rhythms is a life-threatening heart disease, which is treated efficiently by the automated external defibrillator (AED). This work proposes a novel design of the SAA, which includes a k-nearest neighbors model and a subset of 8 features extracted from the ECG segments, for the SCA diagnosis on the electrocardiogram (ECG) signal. These features are addressed as the most productive subset among 31 input features based on the evaluation of the feature correlation. The recursive feature elimination algorithm combined with the Boosting model and wise-patient fivefold cross-validation method is adopted for the calculation of the average feature importance, which shows the degree of feature correlation, to construct various input feature subsets. Moreover, component feature combinations known as the representatives of the input feature subsets with an enormous level of correlation and independence are transformed from the input subsets by the principal component analysis method. The wise-patient fivefold cross-validation procedure is used for the evaluation of these component feature combinations on the validation set. The proposed SAA is certainly efficient for SCA detection with a small number of the extracted feature and relatively high diagnosis performance such as accuracy of 99.52%, sensitivity of 97.69%, and specificity of 99.91%.
Bound excitons and bandgap engineering in violet phosphorus
Authors: Sun Zhenyu , Cai Zhihao , Cheng Peng , Chen Lan , Zhao Xuewen , Zhang Jinying , Wu Kehui , Feng Baojie 
Published: May 2023

AbstractViolet phosphorus (VP), the most stable phosphorus allotrope, is a van der Waals semiconductor that can be used to construct p-type nanodevices. Recently, high-quality VP crystals have been synthesized while a deep insight into their excitonic properties and bandgap tailoring approaches, which are crucial for their optoelectronic device applications, is still lacking. Here, we study the optical properties of ultrathin VP by second harmonic generation, photoluminescence, and optical absorption spectroscopy. We observed strong bound exciton emission that is 0.48 eV away from the free exciton emission, which is among the largest in 2D materials. In addition, the bandgaps of VP are highly sensitive to the number of layers and external strain, which provides convenient approaches for bandgap engineering. The strong bound exciton emission and tunable bandgaps make VP a promising material in optoelectronic devices.
Differential Binding of NLRP3 to non-oxidized and Ox-mtDNA mediates NLRP3 Inflammasome Activation
Authors: Cabral Angela , Cabral Julia Elise , Wang Angelina , Zhang Yiyang , Liang Hailin , Nikbakht Donya , Corona Leslie , Hoffman Hal M. , McNulty Reginald 
Published: May 2023

AbstractThe NLRP3 inflammasome is a key mediator of the innate immune response to sterile tissue injury and is involved in many chronic and acute diseases. Physically and chemically diverse agents activate the NLRP3 inflammasome. Here, we show that NLRP3 binds non-oxidized and Ox-mtDNA differentially, with a half maximum inhibitory concentration (IC50) for non-oxidized and Ox-mtDNA of 4 nM and 247.2 nM, respectively. The NLRP3 Neonatal-Onset Multisystem Inflammatory Disease (NOMID) gain of function mutant could bind non-oxidized mtDNA but had higher affinity for Ox-mtDNA compared to WT with an IC50of 8.1 nM. NLRP3 lacking the pyrin domain can bind both oxidized and non-oxidized mtDNA. Isolated pyrin domain prefers Ox-mtDNA. The NLRP3 pyrin domain shares a protein fold with DNA glycosylases and generate a model for DNA binding based on the structure and sequence alignment toClostridium acetobutylicumand human OGG1, an inhibitor of Ox-mtDNA generation, 8-oxoguanine DNA glycosylases. We provide a new model for how NLRP3 interacts with Ox-mtDNA supported by DNA binding in the presence of a monoclonal antibody against the pyrin domain. These results give new insights into the mechanism of inflammasome assembly, and into the function of reactive oxygen species in establishing a robust immune response.
The SWine IMputation (SWIM) haplotype reference panel enables nucleotide resolution genetic mapping in pigs
Authors: Ding Rongrong , Savegnago Rodrigo , Liu Jinding , Long Nanye , Tan Cheng , Cai Gengyuan , Zhuang Zhanwei , Wu Jie , Yang Ming , Qiu Yibin , Ruan Donglin , Quan Jianping , Zheng Enqin , Yang Huaqiang , Li Zicong , Tan Suxu , Bedhane Mohammed , Schnabel Robert , Steibel Juan , Gondro Cedric , Yang Jie , Huang Wen , Wu Zhenfang 
Published: May 2023

AbstractGenetic mapping to identify genes and alleles associated with or causing economically important quantitative trait variation in livestock animals such as pigs is a major goal in animal genetic improvement. Despite recent advances in high-throughput genotyping technologies, the resolution of genetic mapping in pigs remains poor due in part to the low density of genotyped variant sites. In this study, we overcame this limitation by developing a reference haplotype panel for pigs based on 2259 whole genome-sequenced animals representing 44 pig breeds. We evaluated software combinations and breed composition to optimize the imputation procedure and achieved an average concordance rate in excess of 96%, a non-reference concordance rate of 88%, and an r2 of 0.85. We demonstrated in two case studies that genotype imputation using this resource can dramatically improve the resolution of genetic mapping. A public web server has been developed to allow the pig genetics community to fully utilize this resource. We expect this resource to facilitate genetic mapping and accelerate genetic improvement in pigs.