bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Antifungal activity of Probiotics against candidiasis
Authors: N.S. Shree Abiraami , Narayan Vivek , S Rajesh 
Published: May 2023

Abstract
Aim:
To analyse the antifungal effects of probiotics on Candida Albicans
Materials and methods:
One capsule of commercially available probiotics with 5 billion colony forming units is mixed with 2 ml of sterile water in test tube. Wells are cut in rose Bengal agar, 20µl,50µl,100µl concentration of the solution is placed. Reading was taken after 24hours.Zone of inhibition was noted. Rose Bengal broth, sterilized and 6ml was added in 3 test tubes. Candida suspension was added to all 3-test tube in the range of 5*10 billion CFU/ml.Percentage of dead cells is calculated at wavelength of 600nm at regular time intervals after incubation.
Results:
In agar well diffusion method, zone of inhibition is 20mm in 25µl, 22mm in 50µl and 25mm for 100µl.Among the concentration 50µl is showed to be effective from MIC activity.
Conclusion:
Probiotics have good antifungal property against candida albicans. In future this can be also be used in primary and adjuvant therapy in management of oral candidiasis.
A multicenter, randomized, parallel-controlled clinical trial protocol to evaluate the safety and efficacy of irreversible electroporation compared with radiofrequency ablation for the treatment of hepatocellular carcinoma
Authors: Pan Jinhua , Xu Min , Li Kai , Xu Dong , Jing Xiang , Lu Qiang , Yang Hong , Zhao QIyu , Deng Zhuang , Jiang Tianan 
Published: May 2023

Abstract

Background
At present, the main clinical application of local ablation therapy, such as radiofrequency ablation (RFA), is to heat the tissue to a certain temperature. However, high temperature will cause thermal damage. Irreversible electroporation (IRE) is a novel minimally invasive local ablation technology for tumors. By high-frequency pulse, the tumor cell membrane can be irretrievably perforated, resulting in the destruction of the intracellular environment, which can preserve important structures in the treatment area. However, there are no randomized controlled clinical trials comparing the efficacy of IRE with traditional local ablation in the treatment of liver cancer.
Methods
We will conduct a multicenter, randomized, parallel-controlled non-inferiority clinical trial to compare the efficacy and safety of IRE and RFA for hepatocellular carcinoma (HCC). One hundred and ninety patients with HCC from five academic medical centers will be enrolled. The patients will be randomized into treatment arm (IRE) and control arm (RFA). The primary outcome is the progress -free survival (PFS) and the key secondary outcome is the Overall survival (OS).
Results
Thirty-three patients have completed the 1-month follow-up. These 33 patients (median age, 59.1 years) have a total of 38 tumors, and the mean largest tumor diameter is 3.9 cm. In these 33 patients, no end point was observed for PFS or OS in both groups, and the complete ablation rate was 100% in both groups. The lesions in the IRE group showed obvious shrinkage 1 month after surgery. One major adverse event (AE) was occurred in the control group.
Conclusions
This is the first randomized controlled clinical trial to compare the clinical effects of IRE and RFA. The preliminary results suggest that both RFA and IRE are effective in the treatment of HCC, which can provide strong evidence for the use of IRE in HCC and provide more options for the treatment of patients with HCC.
Clinical Trial Registration ClinicalTrials.gov, identifier NCT05451160.
Association of sun-seeking behaviors with indoor tanning habit in US females
Authors: Seo Bojung , Yang Sheng , Cho Eunyoung , Qureshi Abrar A , Han Jiali 
Published: May 2023

Abstract
Background
Frequent exposure to ultraviolet light in early life has more detrimental and long-term effects on skin than in adulthood. Teenagers with strong sun-seeking behaviors may be more likely to use an indoor tanning bed than those who seek less sun, probably due to addictiveness of ultraviolet exposure. We aimed to examine associations between sun exposure behaviors and average annual indoor tanning usage frequency during high school/college in US females.
Methods
In this cross-sectional study, we used data from The Nurses’ Health Study II, a large prospective cohort of US female nurses. We included a total of 81,746 white females who answered the average annual frequency of indoor tanning during high school/college. Our study exposures were average weekly time spent outdoors in a swimsuit and average percentage of time of wearing sunscreen at the pool or beach during their teenage years, average weekly hours spent outdoors in direct sunlight in daytime during high school/college, and the number of severe sunburns which blistered between ages 15–20. Main outcomes was average annual frequency of indoor tanning bed usage during high school/college.
Results
In multivariable-adjusted logistic regression, we demonstrated positive associations between the sun exposure behaviors and the indoor tanning habit. Specifically, teenagers who spent daily outdoors in a swimsuit (adjusted odds ratio [aOR], 95% confidence interval [CI] for daily vs. <1/week: 2.68, 1.76–4.09) or who had ≥ 10 sunburns that blistered (aOR, 95% CI for ≥ 10 vs. never: 2.18, 1.53–3.10) were more likely to use indoor tanning beds ≥ 12 times/year. Also, teenagers/undergraduates who spent ≥ 5hours/week outdoors in direct sunlight during daytime used indoor tanning ≥ 12 times/year (aOR, 95% CI: 2.18, 1.39–3.44) than those who spent < 1/week. However, there was not a significant association between average uses of sunscreen at the pool/beach and indoor tanning bed. Multivariable-adjusted linear regression models also showed similar results.
Conclusions
Teenagers who spent more time outdoors or got more sunburns tended to use indoor tanning more frequently. These findings provide evidence that teenagers with strong sun-seeking behaviors may have excessive exposure to artificial ultraviolet radiation as well.
IL12B and IL17 genes polymorphisms are associated with different susceptibility to Juvenile Idiopathic Arthritis and Juvenile-onset Systemic Lupus Erythematosus in Chinese children
Authors: Zhang Menglan , Peng Leiwen , Li Wensheng , Duan Yifei , Liu Xiaoqin , Chen Shasha , Deng Jiamin , Liu Xinle 
Published: May 2023

Abstract
Background
Genetic factors play a crucial role in the immune response of juvenile idiopathic arthritis (JIA) and juvenile-onset systemic lupus erythematosus (JSLE). This study aimed to investigate the association of IL12B (rs3212227, rs6887695) and IL17 (rs2275913, rs763780) gene polymorphisms with the susceptibility of JIA and JSLE in Chinese children.
Methods
A total of 303 healthy controls and 304 patients including 160 JIA and 144 patients were analyzed, and the genetic polymorphisms were genotyped by using a Sequenom MassArray system.
Results
There was a significant association between the IL12B rs3212227 genotype and the increased risk of JSLE (p = 0.01). For rs6887695, the minor allele C was significantly associated with the increased risk of JIA (odds ratio = 1.48, 95% CI = 1.12–1.95, p = 0.005). Moreover, rs6887695 genotype was significantly associated with both JIA and JSLE susceptibility (p < 0.05). Besides, IL12B haplotype GC significantly associated with the increased risk of JIA (p = 0.016). However, no significant difference was found between the IL17 (rs2275913, rs763780) gene polymorphisms and JIA or JSLE susceptibility (p > 0.05). And similar genotype distributions of IL12B and IL17 polymorphisms were found between the patients with nephritis and without nephritis in JSLE (p > 0.05).
Conclusion
Our results indicated that IL12B polymorphisms was associated with an increased risk for the development of JIA and JSLE in Chinese children, highlighting the involvement of inflammation in the pathogenesis of JIA and JSLE. Moreover, there was a risk haplotype in IL12B which could increase the risk of JIA.
Enhanced imatinib uptake through influx transporters and its quantification based on LC-MS/MS in human leukemic cells
Authors: GUPTA SONU KUMAR , Kumari Nisha Bala , Saini Khem Chand , Singh Priyanka , Chhabra Ravindresh , Bhardwaj Monika , Grover Parul , Verma Malkhey 
Published: May 2023

Abstract
Chronic myeloid leukemia (CML) is a slowly progressing cancer of the blood cells that originates in the pluripotent hematopoietic stem cells. It is caused by the reciprocal translocation between chromosomes 9 and 22, which results in the formation of a chimeric chromosome referred to as the ‘Philadelphia (Ph) chromosome’. As per GLOBOCAN 2020, leukemia is the tenth most frequently occurring cancer across the world with an overall mortality rate of 3.1%. Among all the countries, India is ranked third in the incidence as well as mortality associated with leukemia. Tyrosine kinase inhibitors (TKIs) are the standard treatment drugs for CML. However, around 30% of CML patients have been observed to develop resistance against imatinib because of the reduced intracellular availability of the drug. Therefore, the present study was designed to enhance the imatinib import through the use of MayBridge fragment (MBF) compounds. Here we used the previously developed imatinib-resistant leukemia cells (K562-R) for further study. The gene expression analysis revealed that the effect of MBF compounds on imatinib treatment was probably due to the change in the expression of import transporters (OATP1A2 and OCT1). The metabolite profiling carried out by LC-MS/MS confirmed that the intracellular concentration of imatinib did increase in K562-R cells when treated with either of the MBFs and imatinib together in comparison to imatinib treatment alone. These results imply that MBF1 and MBF4 used in this study enhance the imatinib import in K562-R cells by altering the expression of membrane transporters in a better way.
GDF-15: A novel biomarker of heart failure predicts 30-day all-cause mortality and 30-day HF rehospitalization in patients with acute heart failure syndrome
Authors: Kosum Paisit , Mattanapojanat Natthinee , Siranart Noppachai , Kongruttanachok Narisorn , Sinphurmsukskul Supanee , Siwamogsatham Sarawut , Puwanant Sarinya , Ariyachaipanich Aekarach 
Published: May 2023

Abstract
Background: Growth Differentiation Factor-15 (GDF-15) is a distant member of the transforming growth factor-β. The increased expression of GDF-15 has been observed during heart failure and is associated with worse outcomes. However, the relationship between GDF-15 and acute heart failure (AHF) is not well understood with limited evidence among Thai patients.
Purpose: To study the association between cumulative level and biomarker at the time of admission and discharge in patients with AHF and their association with 30-day all-cause mortality and heart failure rehospitalization
Methods: This is a prospective, single-center study of patients admitted for AHF. GDF-15, high-sensitivity troponin (hsTrop) and NT-proBNP were measured at the time of admission and discharge. Outcomes, including 30-day all-cause mortality and rehospitalization, were collected. Logistic regression analysis was used to evaluate outcomes.
Results: A total of 84 patients were enrolled (mean age of 69 years, 52% females). The GDF-15 level significantly decreased during admission (at the time of admission 9,118 pg/mL, at the time of discharge 7,107 pg/mL, mean differences 1528; p = 0.006). The 30-day all-cause mortality was 6%. The 30-day rehospitalization occurred in 22 (26.1%) patients. Univariate analysis showed that the admission GDF-15 level, but not hsTrop or NT-proBNP, was associated with 30-days all-cause mortality (p = 0.029) and multivariate analysis showed a reduction in GDF-15 level from admission to discharge was associated with lower 30-days rehospitalization (p = 0.023).
Conclusion: The GDF-15 levels were elevated during acute HF and improved by the time of discharge. This novel biomarker may offer additional value in predicting outcomes in acute HF.
White matter anatomy of human brain using DTI based atlas: A pictoral review at 3T
Authors: Khan Beenish , Dixit Rashmi 
Published: May 2023

Abstract
Purpose:
DTI is frequently used in the diagnostic radiology in all domains including congenital white matter abnormalities, tumours, infections, demyelination, psychiatric illnesses. To interpret pathology involving white matter, one must be aware of normal appearance of the white matter fibres of the brain. For neurosurgery, awareness of normal appearing white matter tracts on DTI is important as DTI based neuronavigation is increasingly being used. We thus aimed to generate colour coded maps of human brain to delineate white matter anatomy using 3T Diffusion Tensor MRI.
Method
Five healthy volunteers underwent MRI on a 3T MR scanner (Magnetom skyra) using 20 channel head coil. Apart from basic MR sequences (T1W, T2W images to rule out any gross pathology) multidimensional diffusion weighted images were obtained in 20 non collinear directions using two b values (b value = 0 and 1000 s/mm2). The DTI data was autotransferred to a Siemens’ work station and post-processed with syngovia software (VB30A_HF06) to generate colour coded FA maps. The images were also manually processed using the same syngovia software to generate three dimensional tractography images.
Results
On DTI, major white fibres that were identified were blue coded (cranio-caudal orientation) projection fibres, red coded commissural fibres (medio-laterally oriented) and green coded association fibres (anteroposteriorly oriented). All major fibres were separately identified and demonstarated using tractography.
Conclusion
DTI is an effective modality to study the anatomy of white matter tracts of human brain and can help radiologists, neurologists, neurosurgeons as well as anatomists to understand the three dimensional orientation of white matter tracts in brain.
Choroidal changes in patients with diabetic retinopathy
Authors: Peng Shu-Yu , Chen Ta-Ching , Hsieh Yi-Ting , Ho Tzyy-Chang , Yang Chung-May , Yang Chang-Hao 
Published: May 2023

Abstract
This study sought to investigate the characteristic choroidal changes in patients with diabetic retinopathy and to identify factors affecting choroidal thickness (CTh), choroidal vascular index (CVI), and choriocapillaris flow in these patients. In this study, we retrospectively analyzed 79 eyes from 53 patients with diabetes from August 2021 to February 2022. We collected laboratory data, including HbA1c, serum creatinine, BUN, triglycerides, total cholesterol, high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL). The measurements of the OCT images, including foveal avascular zone (FAZ), retinal vascular density, choroidal flow, retinal thickness, CTh and CVI, were analyzed. The possible determining factors affecting CTh, CVI, and choriocapillaris flow were analyzed using nonparametric multivariate analysis.We found that LDL (P<.001) was positively correlated with CTh, while CVI (P=.007) had a negative correlation with CTh in diabetic patients. Our study also identified a negative correlation between choriocapillaris flow and DVD (P=.018) in low-grade DR patients, which was not identified in patients with more advanced DR.
Improve the Quality of Recommender Systems based on Collaborative Filtering with Missing Data Imputation
Authors: Hikmawati Erna , Nugroho Heru , Surendro Kridanto 
Published: May 2023

Abstract
Recommender systems which are becoming increasingly popular and have been proven to boost cross-selling and customer satisfaction still need further refinement to improve their relevancy and correspond to the user’s need. One widely used method of recommender systems is Collaborative Filtering (CF). The CF method bases predictions for a user on feedback data from other users, so that user preferences can be formulated based on the feedback it provides. One of the prevalent obstacles to the CF method is that the feedback data is often incomplete because there is no compulsion for the user to provide feedback on the items they have consumed. Incomplete data will affect the quality of recommendation results. One way to address this is to use missing data imputation. In this study, a collaborative filtering method is proposed where missing data imputation is carried out first before commencing data training. From the experiment results using Amazon Customer Review dataset, it is shown that the CF method with Imputation Mean produces lower RMSE and MAE values.
Vitamin D Status, Vitamin D Receptor Polymorphisms, and the Risk of Incident rosacea: Evidence from mendelian randomization and Prospective Study of the UK Biobank
Authors: MAO RUI , Zhou Guowei , Jing Danrong , Liu Hong , Shen Minxue , Li Ji 
Published: May 2023

Abstract
Existing cross-sectional studies can not establish a causal relationship between serum 25-hydroxyvitamin D (25OHD) status and incident rosacea. We aim to prospectively investigate the association of serum 25OHD and vitamin D receptor (VDR) polymorphisms with the risk of incident rosacea. The study included 370,209 individuals from the UK biobank. Cox proportional hazard regression models were used to estimate hazard ratios (HRs) and 95%CI. Two-sample Mendelian randomization (MR) analyses were applied to help elucidate the causality between 25OHD and rosacea. During a mean follow-up of 13.22 years, we documented 1,938 cases of rosacea. Higher levels of serum 25OHD were significantly associated with a lower risk of incident rosacea. Compared with participants with 25OHD <25 nmol/L, multivariate-adjusted HR for incident rosacea was 0.81 (95% CI: 0.70, 0.94) in individuals with 25OHD>50 nmol/L. Compared with participants with serum 25OHD less than 25nmol/L and the allele of rs731236 (TaqI) AA, those with serum 25OHD greater than 75nmol/L and the allele of TaqI GG had a multivariate-adjusted HR of 0.51 (95%CI 0.32 to 0.81) for incident rosacea. The results of the Mendelian randomization (MR) study suggest that 25OHD is associated with a 23% decreased risk of rosacea (HR = 0.77, 95%CI: 0.63, 0.93). In general, Higher serum 25OHD concentration is associated with a lower risk of incident rosacea.
Loss of PEX26 promotes migration, invasion, and EMT via activating Wnt pathway in colorectal cancer
Authors: Yan Bianbian , Gao Liyang , Wei Shangqing , Wang Mengwei , Cao Lichao , Tian Ye , Ren Yanan , Jiang Jingjing , Yang Jin , Chen Erfei 
Published: May 2023

Abstract
Introduction
The role of PEX26 in colorectal cancer (CRC) development remains unknown. We aimed to study PEX26 expression, regulation, and function in CRC cells.
Methods
By using the databases analysis, real-time quantitative PCR, and immunohistochemistry staining we detected that the expression of PEX26 in CRC and normal tissue. The effect of PEX26 on CRC cells and regulatory mechanism are conducting by functional experiments in vitro.
Results
PEX26 are significantly down-regulated in CRC tissue, and its low expression correlates with the poor overall survival of CRC patients. We further demonstrated that PEX26 over-expression inhibit the ability of CRC cell migration, invasion, and epithelial mesenchymal transition (EMT), while PEX26 knock-down promotes the malignant phenotypes of migration, invasion, and EMT via activating the Wnt pathway.
Conclusions
Overall, our results showed that the loss of PEX26 contributes to the malignant phenotype of CRC. PEX26 may serve as a novel metastasis repressor for CRC.
Efficacy of botulinum toxin in the management of refractory de novo overactive bladder symptoms in women after midurethral sling placement (ADVANCE- Study)
Authors: Hijazi Sameh , Karapanos Leonidas , Halbe Luisa , Heidenreich Axel , Hasselhof Viktoria , Alkhalfa Amer , Barakat Bara 
Published: May 2023

Abstract
Purpose To evaluate the efficacy of onabotulinumtoxin A (onaBTX-A) intradetrusor injections in women with refractory de novo overactive bladder (OAB) following midurethral sling (MUS) placement.Methods A retrospective single-center study was conducted. We screened 372 women who underwent MUS surgery between August 2009 and January 2022. 54/372 women diagnosed with pharmacologically refractory de novo OAB following MUS were evaluated using cystoscopy and urodynamics, and after tape erosion and obstructive voiding were excluded, they received onaBTX-A therapy. Outcomes were the reduction of self-reported OAB symptoms and leakage episodes, improvement of validated OAB scores and adverse events of the procedure after a follow-up of 3, 6, and 12 months.Results Successful results were reported in 81%, 68%, and 43% at 3, 6 and 12 months respectively. Postoperatively, median voiding frequency and median nocturia episodes were significantly improved in 70% and 77% of women, respectively, with a decrease in daily number of voids (-4.1, p = 0.0001) and nocturia episodes (-2.2, p = 0.005). At 3 months, 80% of women reported an > 25% reduction in urgency severity and episodes following injection. The median number of pads used was significantly reduced after injection (-2 pads; p = 0.03). Repeat injections of onaBTX-A were performed in 61% of patients after a median of 11 months.Conclusions Intravesical onaBTX-A injections are clinically effective at 3- and 6-month follow-up for the treatment of refractory de novo OAB after MUS placement. Over 60% of the patients opted for retreatment with onaBTX-A due to a high level of satisfaction.
Bioinformatics and in vitro-based comprehensive analysis of EVI2A expression and its immunological and prognostic significance in Kidney Renal Clear Cell Carcinoma
Authors: Li Sheng , Zheng Fucun , Xiong Situ , Yang Lin , Jiang Ming , Liu Jiahao , Liu Xiaoqiang , Xu Songhui , Zeng Jin , Fu Bin 
Published: May 2023

Abstract
Background
Previous studies have shown that the Ecotropic Viral Integration Site 2A (EVI2A) could serve as a meaningful marker in many diseases, yet its potential biological function and mechanism in kidney renal clear cell (KIRC) carcinoma have not been investigated.
Methods
TCGA and GEO databases were used for EVI2A gene expression and pan-cancer analysis. We used Kaplan-Meier (K-M) analysis, receiver operating characteristic (ROC) curves, and nomogram to assess the clinical utility of EVI2A. In parallel, we evaluated the immune relevance of the gene by tumor microenvironment (TME), Tumor Immune Single-cell Hub (TISCH), immune checkpoint, and immunotherapy sensitivity analysis. Finally, the expression of this gene was verified in vitro assay and further verified the biological behavior in renal clear cell carcinoma by cell function experiments.
Results
EVI2A expression in KIRC were upregulated and associated with patients’ tumor grade, T /N/M stage. The diagnostic AUC of EVI2A was 0.906. Its high expression indicated poor overall survival and progression-free survival in KIRC patients. Both GO, and KEGG analysis indicated significant correlations between EVI2A expression and immunity. The higher the EVI2A expression, the higher the TME scores. Furthermore, EVI2A was positively correlated with Tfh cells, CD4 memory T cells and CD8 + T cells. Patients with high expression of EVI2A are more sensitive to PD-1/CTLA-4 and tyrosine kinase inhibitors. In vitro experiments showed that the knockdown of EVI2A reduced KIRC cell proliferation, invasion, and migration.
Conclusion
Comprehensive analysis indicated that EVI2A may be a potential meaningful biomarker and novel target for KIRC intervention.