bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms of active diffusion of vesicular stomatitis virus inclusion bodies and cellular early endosomes in the cytoplasm of mammalian cells
Authors: Moran Steven J. , Oglietti Ryan , Smith Kathleen C. , Macosko Jed C. , Holzwarth George , Lyles Douglas S. 
Published: August 2023

ABSTRACTViral and cellular particles too large to freely diffuse have two different types of mobility in the eukaryotic cell cytoplasm: directed motion mediated by motor proteins moving along cytoskeletal elements with the particle as its load, and motion in random directions mediated by motor proteins interconnecting cytoskeletal elements. The latter motion is referred to as “active diffusion.” Mechanisms of directed motion have been extensively studied compared to mechanisms of active diffusion, despite the observation that active diffusion is more common for many viral and cellular particles. Our previous research showed that active diffusion of vesicular stomatitis virus (VSV) ribonucleoproteins (RNPs) in the cytoplasm consists of hopping between traps and that actin filaments and myosin II motors are components of the hop-trap mechanism. This raises the question whether similar mechanisms mediate random motion of larger particles with different physical and biological properties. Live-cell fluorescence imaging and a variational Bayesian analysis used in pattern recognition and machine learning were used to determine the molecular mechanisms of random motion of VSV inclusion bodies and cellular early endosomes. VSV inclusion bodies are liquid-liquid phase separated compartments that are the major sites of viral RNA synthesis, and early endosomes are representative of cellular membrane-bound organelles. Like VSV RNPs, inclusion bodies and early endosomes moved from one trapped state to another, but the distance between states was inconsistent with hopping between traps, indicating that the apparent state-to-state movement is mediated by trap movement. Like VSV RNPs, treatment with the actin filament depolymerizing inhibitor latrunculin A increased VSV inclusion body mobility by increasing the size of the traps. In contrast neither treatment with latrunculin A nor depolymerization of microtubules by nocodazole treatment affected the size of traps that confine early endosome mobility, indicating that intermediate filaments are likely major trap components for these cellular organelles.
A critical period for prefrontal cortex PV interneuron myelination and maturation
Authors: Hijazi S. , Pascual-García M. , Nabawi Y. , Kushner S.A. 
Published: August 2023

AbstractRecent studies have highlighted axonal myelination as a common feature of parvalbumin-positive (PV) interneurons throughout the cerebral cortex. However, the precise function of PV interneuron myelination remains incompletely understood. In this study, we used the cuprizone model of demyelination to investigate how PV interneuron myelination might influence their neuronal physiology. Specifically, we examined whether impairing myelination from postnatal day 21 onwards, during a critical neurodevelopmental period of the prefrontal cortex (PFC), can affect PV interneuron maturation and function. Using whole-cell patch-clamp recordings to examine intrinsic properties of PV interneurons in the PFC, we found that juvenile demyelination induced robust alterations of PV interneuron firing patterns. Specifically, we observed that demyelination caused an impairment in the ability of PV interneurons to sustain high frequency firing associated with a substantial decrease in Kv3-specific currents. We also found a significant impairment in PV interneuron autaptic self-inhibitory transmission, a feature implicated in temporal control of PV interneuron firing during cortical network activity. Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered and mice showed clear social deficits, suggesting that transient juvenile demyelination leads to long-lasting behavioral impairments. In contrast, adult demyelination had no significant effects on PV interneuron firing properties. Together, our data uncovers a critical period for juvenile myelination as an important factor in PFC PV interneuron development and brain maturation.
Ecosystems of doing business and living standards: a configurational analysis based on Chinese cities
Authors: Li Jiaxin , Du Yunzhou , Sun Ning , Xie Zhimin 
Published: August 2023

Purpose
This paper aims to explain the causal complexity between ecosystems of doing business and living standards based on the theoretical model of the ecosystem of doing business proposed by Li (2019) and Du et al. (2020). By integrating ecological theory, transaction cost theory and institutional logics theory, this study explored effective ecosystems of doing business that achieve high living standards and explained the interpretive mechanisms behind different ecosystems of doing business. Moreover, this study also analyzed whether there were any necessary elements that lead to high living standards and discussed how the interactions between these elements influence carrying capacity and transaction costs from government logic and market logic, thus affecting living standards.

Design/methodology/approach
In this study, fuzzy set qualitative comparative analysis (fsQCA) and necessary condition analysis (NCA) were combined to analyze the data from the 2020 China City Statistical Yearbook, covering the main socioeconomic statistical data of cities at all levels in 2019.

Findings
This study found that no individual factor of the ecosystems of doing business was necessary to achieve high living standards, but the high level of human capital, innovation capacity, financial access and market demand play a significant role in achieving high living standards. Furthermore, two effective types of ecosystems of doing business lead to high living standards, namely, market dominance (government’s “invisible hand” or “nudging hand”) and government–market logic mutualism/symbiosis (government’s “helping hand”).

Originality/value
First, this work found that individual elements were not a necessary condition for high living standards, not only in kind but also in degree, complementing fsQCA with NCA, which indicates that environmental elements can be substituted by others. Second, this study considered the complex effects and explained the mechanisms behind different ecosystems of doing business, drawing on ecological theory, transaction cost theory and institutional logics theory from a configurational perspective. This study deepened the theories’ applications in the field of living standards and further discussed the elements interactions. Third, this study introduced configurational perspective and QCA into living standards research and adopted a mixed method that combines fsQCA and NCA to analyze the causal complexity between ecosystems of doing business and people’s living standards.
Proposed standard guideline for managing information systems (ISs) in libraries in Africa
Authors: Ocloo Patience Emefa Dzandza , King  Lizette 
Published: August 2023

Purpose
Libraries in Africa are adopting technology at a fast pace. However, literature has provided enough evidence of the many challenges libraries on the continent are facing in the use of the adopted information technology of which information systems (ISs) are major components. Literature also does not provide evidence of IS standard, policy or guideline at both national and international levels as a benchmark for the use of these systems. Therefore, this paper aims to propose an IS standard guideline as a guide for libraries in Africa to develop IS policies, standards or guidelines to help in effective use of IS in libraries.

Design/methodology/approach
The researchers based their proposed standard guideline on the findings from their earlier research works and evidence from other existing literature as has been reviewed in this paper.

Findings
Findings from the literature revealed that libraries in Africa face a number of challenges in the use of IS which can be categorised into four main areas, namely, financial challenges, technological and infrastructural challenges, human resource challenges and organizational challenges.

Originality/value
This paper proposed an IS standard guideline to serve as a guide for libraries in Africa to develop IS policies, standards or guidelines to help in effective use of IS in libraries.
The mediating role of planned behaviour in the religiosity and nascent entrepreneurship nexus
Authors: Onjewu Adah-Kole Emmanuel , Anosike Paschal , Godwin Eun Sun 
Published: August 2023

PurposeIncreasingly, there is scholarly recognition that individuals' faith constitutes a background factor much like other antecedents conditioning entrepreneurial inclination. Yet, there is room to expand knowledge on how faith interrelates with psychological and social determinants of entrepreneurship, especially in under-researched contexts such as Nigeria.Design/methodology/approachThis inquiry conceptualises associations between religiosity and (1) entrepreneurial self-efficacy, (2) entrepreneurial attitudes (3) and subjective norms as predictors of nascent entrepreneurship. For analysis, 1,259 observations of Nigerian students are assessed by structural equation modelling.FindingsThe path analysis showed that the religiosity–nascent entrepreneurship nexus is altogether mediated by entrepreneurial self-efficacy, entrepreneurial attitudes and subjective norms. Entrepreneurial self-efficacy is found to have the greatest impact on nascent entrepreneurship, followed by subjective norms and then entrepreneurial attitudes.Originality/valueTheoretically, this study is one of the first to test all three dimensions of the theory of planned behaviour in the religiosity–nascent entrepreneurship nexus. It draws fresh attention to faith motivation and praxis, role-taking and attribution theory as explainers of the inherent correlations. Practically, the findings summon stakeholders to consider religious activity in the delivery of entrepreneurship programmes.
Multiobjective network security dynamic assessment method based on Bayesian network attack graph
Authors: Xie Jialiang , Zhang Shanli , Wang Honghui , Chen Mingzhi 
Published: August 2023

PurposeWith the rapid development of Internet technology, cybersecurity threats such as security loopholes, data leaks, network fraud, and ransomware have become increasingly prominent, and organized and purposeful cyberattacks have increased, posing more challenges to cybersecurity protection. Therefore, reliable network risk assessment methods and effective network security protection schemes are urgently needed.Design/methodology/approachBased on the dynamic behavior patterns of attackers and defenders, a Bayesian network attack graph is constructed, and a multitarget risk dynamic assessment model is proposed based on network availability, network utilization impact and vulnerability attack possibility. Then, the self-organizing multiobjective evolutionary algorithm based on grey wolf optimization is proposed. And the authors use this algorithm to solve the multiobjective risk assessment model, and a variety of different attack strategies are obtained.FindingsThe experimental results demonstrate that the method yields 29 distinct attack strategies, and then attacker's preferences can be obtained according to these attack strategies. Furthermore, the method efficiently addresses the security assessment problem involving multiple decision variables, thereby providing constructive guidance for the construction of security network, security reinforcement and active defense.Originality/valueA method for network risk assessment methods is given. And this study proposed a multiobjective risk dynamic assessment model based on network availability, network utilization impact and the possibility of vulnerability attacks. The example demonstrates the effectiveness of the method in addressing network security risks.
Digital quality’s role in US online higher education
Authors: Chakraborty Subhajit 
Published: August 2023

Purpose
The emergence of internet-based business models has given rise to online higher education institutions (OHEIs) that offer their undergraduate and graduate degree programs exclusively online with minimal physical presence. Research on OHEIs discusses the need for external legitimacy and resource acquisition, often ignoring the role of quality among these institutions. Hence, this study aims to investigate the role of digital quality of education on OHEIs’ survival.

Design/methodology/approach
Guided by four different inter-disciplinary theories, a conceptual framework is offered based on a comprehensive literature review.

Findings
The role of digital quality of education in improving the survival and strategic competitiveness of institutions in the US online higher education industry is highlighted.

Research limitations/implications
This conceptual paper highlights how the digital quality of education becomes increasingly important over the life cycle of OHEIs.

Practical implications
The proposed framework suggests that despite the competition provided by traditional and well-entrenched players, OHEIs can improve their survival and competitiveness if they invest strategically in the digital quality of education.

Originality/value
This study offers an overarching conceptual framework developed through an integration of multiple theoretical perspectives and grounded in the US online higher education industry.
The crowding‐out effects of innovation information disclosure on peers' innovation: Evidence from innovation‐driven M&As in China
Authors: Cao Zhangfan , Hua Chen , Tong Yan , Wang Xi 
Published: August 2023

AbstractThis study investigates the impact of rivals' announcements of innovation‐driven mergers and acquisitions (M&As) on focal firms' ex‐post corporate innovation performance. Exploiting a hand‐collected dataset of Chinese listed firms from 2011 to 2018, we find that focal firms file fewer patents after rivals' announcements of innovation‐driven M&As. Further analyses show that focal firms become more conservative and hold more cash when rivals intend to obtain innovation resources through M&As. We find that such crowding‐out effects are more pronounced for focal firms that are more financially constrained, those facing higher competition, those whose rivals are industry leaders, and those operating in traditional industries.
Effect of antifungal compounds secreted by Lactiplantibacillus plantarum 124 against Aspergillus flavus and Penicillium sp. and its application in Kaeng‐Tai‐Pla‐Haeng to extend the shelf life
Authors: Kanjan Pochanart , Sakpetch Phat 
Published: August 2023

SummaryAspergillus flavus and Penicillium spp. are the most common spoilage fungi in the ready‐to‐eat, intermediate‐moisture Thai curry of Kaeng‐Tai‐Pla‐Haeng, which causes serious economic loss and public health concerns. The use of lactic acid bacteria (LAB) with antifungal activity has been a growing interest in food bio‐preservation. In this study, the antifungal efficacy of Lactiplantibacillus plantarum 124 (L. plantarum 124) against the spoilage fungi Aspergillus flavus and Penicillium sp. was evaluated. The cell‐free supernatants (CFSs) of L. plantarum 124 showed strong inhibition against A. flavus and Penicillium sp., with MIC ranging from 11.25 to 22.5 mg mL−1. The significant loss of antifungal activity was observed by pH neutralisation, whereas either heat or proteinase K treatment showed no influence. The major compounds present in CFSs were lactic acid (49.57%) and acetic acid (25.85%). Others, such as 2,3‐dimethyl‐5‐ethylpyrazine (6.07%), 2,5‐dimethyl‐3‐propylpyrazine (2.10%), Cyclo(D‐Leu‐L‐Pro) (2.30%), Ethyl 5‐oxopyrrolidine‐3‐carboxylate (1.84%), and palmitic acid (1.80%), were detected in low relative amounts. As observed by electron microscopy, the CFSs secreted by L. plantarum 124 caused severe damage to cell membranes and organelles of A. flavus and Penicillium sp. In addition, L. plantarum 124 supernatant demonstrated no cytotoxicity on Vero cells, and it could extend the food product's shelf life from 7 days to 21 days without any commercial preservatives. These results demonstrate that antifungal metabolites secreted by L. plantarum 124 are candidates for the development of bio‐preservative agents.
SiC modified self‐healing ytterbium disilicate materials for potential environmental barrier coating application
Authors: Du Jinping , Wang Yanfei , Wan Fan , Li Junsheng , Li Duan , Liu Rongjun 
Published: August 2023

AbstractEnvironmental barrier coatings (EBCs) are crucial to the reliability and durability of SiCf/SiC composite components seeking applications in hot sections of next‐generation advanced aero‐engines. The cracks initiated and developed in EBCs owing to various reasons during service greatly undermine their lifespans. To address this problem, in this work, silicon carbide (SiC) in the forms of particles and whiskers with various amounts have been introduced to ytterbium disilicate (Yb2Si2O7), the mainstream EBC topcoat materials, so as to gain some self‐healing potential. The results reveal that, the SiC inclusions in Yb2Si2O7 in the presence of ytterbium monosilicate (Yb2SiO5) can trigger the following reactions. Specifically, SiC self‐healing agents are oxidized to form viscous SiO2, which actively reacts with Yb2SiO5 upon encountering it, forming Yb2Si2O7. This has brought twofold beneficial effects including ① silicon supplementation of disilicate topcoat, whose silicon element tends to be “dragged out” by water vapor, leading to the deterioration of thermal mismatch; as well as ② crack self‐healing resulting from the volume expansion induced by the above reactions. Then the two aspects of self‐healing agents, namely the “promptness” and “sustainability,” have been discussed in detail. The former is unveiled to be more pertinent to the repairing of large cracks, whilst the latter is more relevant to the self‐healing of tiny cracks at initiation or early stage of propagation. The current work sheds some lights on the design and development of more durable and robust EBCs with self‐healing capability.
European Neuroendocrine Tumor Society (ENETS) 2023 guidance paper for appendiceal neuroendocrine tumours (aNET)
Authors: Kaltsas Gregory , Walter Thomas , Knigge Ulrich , Toumpanakis Christos , Santos Ana P. , Begum Nehara , Pape Ulrich F. , Volante Marco , Frilling Andrea , Couvelard Anne 
Published: August 2023

AbstractThe aim of the present guidance paper is to update the previous ENETS guidelines on well differentiated appendiceal neuroendocrine tumours (NET), providing practical guidance for the diagnosis and management of appendiceal NET (aNET); poorly differentiated neoplasms are dealt with in a separate guidance paper. This paper is structured on a question‐answer format in order to also address controversial issues and areas where uncertainty regarding the management and follow‐up of aNET exists. All recommendations are offered on the basis of the best available evidence, along with the authors' experiences in managing these neoplasms. Each recommendation for treatment will provide a level of evidence and grade of recommendation as per the GRADE system (adapted in Infectious Disease Society of United States Public Health Service grading system).
Expanding the genetic and clinical spectrum of SORD‐related peripheral neuropathy by reporting a novel variant c.210T>G and evidence of subclinical muscle involvement
Authors: Li Lu , Xie Yongzhi , Zeng Sen , Li Xiaobo , Lin Zhiqiang , Huang Shunxiang , Zhao Huadong , Cao Wanqian , Liu Lei , Liu Jun , Rong Pengfei , Zhang Ruxu 
Published: August 2023

AbstractBackground and AimsBiallelic variants in the sorbitol dehydrogenase (SORD) gene have been identified as the genetic cause of autosomal recessive (AR) peripheral neuropathy (PN) manifesting as Charcot–Marie–Tooth disease type 2 (CMT2) or distal hereditary motor neuropathy (dHMN). We aim to observe the genetic and clinical spectrum of a cohort of patients with SORD‐related PN (SORD‐PN).MethodsA total of 107 patients with AR or sporadic CMT2/dHMN underwent molecular diagnosis by whole‐exome sequencing and subsequent Sanger sequencing validation. Available phenotypic data for SORD‐PN were collected and analyzed.ResultsEleven (10.28%) of 107 patients were identified as SORD‐PN, including four with CMT2 and seven with dHMN. The SORD variant c.210 T > G;p.His70Gln in F‐d3 was firstly reported and subsequent analysis showed that it resulted in loss of SORD enzyme function. Evidence of subclinical muscle involvement was frequently detected in patients with SORD‐PN, including mildly to moderately elevated serum creatine kinase (CK) levels in 10 patients, myogenic electrophysiological changes in one patient, and muscle edema in five patients undergoing lower extremity MRI. Fasting serum sorbitol level was 88‐fold higher in SORD‐PN patients (9.69 ± 1.07 mg/L) than in healthy heterozygous subjects (0.11 ± 0.01 mg/L) and 138‐fold higher than in healthy controls (0.07 ± 0.02 mg/L).InterpretationThe novel SORD variant c.210 T > G;p.His70Gln and evidence of subclinical muscle involvement were identified, which expanded the genetic and clinical spectrum of SORD‐PN. Subclinical muscle involvement might be a common but easily overlooked clinical feature. The serum CK and fasting serum sorbitol levels were expected to be sensitive biomarkers confirmed by follow‐up cohort study.
Endogenous choice of price or quantity contract with upstream advertising
Authors: Hu Qing , Li Dan , Mizuno Tomomichi 
Published: August 2023

AbstractWe investigate a supply chain comprising a manufacturer engaged in advertising and two retailers who compete with differentiated products. We examine the endogenous choice between competing on quantity or price for the retailers. Our analysis reveals that, depending on the level of product substitutability, the range of possible outcomes is varied and includes Cournot, Bertrand, and Cournot‐Bertrand under informative advertising. This result contradicts the established understanding that firms tend to engage in Cournot competition as their dominant strategy. Furthermore, we find that under persuasive advertising, Cournot or Bertrand outcomes may be optimal, but Cournot‐Bertrand never arises as an equilibrium.