bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Daclatasvir and Half Dose Sofosbuvir in the Treatment of Hepatitis –C Virus Infection in Patients of Maintanance Hemodialysis-3 Years Trial in A Tertiary Renal Center
Authors: Jabin Masrura , Mostafi Mamun , Kaiser Amir Mohammad , Samir Mohammad Raidul Kader , Linkon Tanvir Chowdhury , Alamgir Saad Bin , Ghosh Himadri , Juthi Shornolota 
Published: March 2023

Background: Hepatitis C virus (HCV) infection in end-stage renal disease (ESRD) heralds a bad outcome. Directly acting antiviral (DAA) drugs sofosbuvir along with daclatasvir are very effective in the management of HCV infection. Sofosbuvir is excreted mainly through the kidneys. There is sparse data worldwide on the use of sofosbuvir based drug regimens in ESRD patients having chronic hepatitis C (CHC) virus infection.This study was designed to evaluate the efficacy of half dose sofosbuvir in the management of HCV infection in ESRD patients on maintenance hemodialysis (MHD).
Methods: This clinical trial was conducted among 125 ESRD patients on MHD at Gonoshasthaya dialysis center, Dhanmondi, Dhaka from July 2019 to June 2022. Total 125 HCV positive patient with ESRD on MHD were included in this study; all the patients underwent HCV-RNA PCR test. Patients with detectable HCV RNA were observed for six months without antiviral drugs to identify the occurrence of spontaneous clearance of virus. Patients who had detectable HCV-RNA after six months were treated with sofosbuvir(200 mg) and daclatasvir (60 mg), irrespective of genotype. The drugs were given daily for 12 weeks. All the patients were on regular follow up at two weeks interval. Blood counts, liver function, creatinine phosphokinase and serum amylase values were evaluated periodically; virological response was assessed by HCV-RNA after 12 weeks of antiviral treatment.
Results: During the observation period, 29 (23.2%) patients had spontaneous virus clearance with an undetectable HCV-RNA. In the remaining 96 patients, the median HCV-RNA level was 2.76×104 (1.56 ×103– 1.89×106)I IU. Twelve weeks after the treatment, 91 (94.8%) patients achieved sustained virological response (SVR) with undetectable HCV-RNA. All patients tolerated the DAAs well and none of the patients reported any serious adverse events. No patient discontinued antiviral therapy due to side effects. Patients who attained SVR with DAA, after 6 months we repeated HCV-RNA in 30 patients, in majority of them (28, 93.3%), HCV-RNA were undetected but in 2 (6.7%) patients,HCV-RNA were detected again.
Conclusion: Daclatasvir along with half-dose sofosbuvir are safe and effective in the treatment of CHC patients with ESRD on MHD. Half dose sofosbuvir regimen can reduce the cost of treatment of HCV in ESRD patients in developing countries like Bangladesh, where cost is a significant barrier to HCV treatment.
J Bangladesh Coll Phys Surg 2023; 41: 102-107
Association of Post-Partum Depression (PPD) with Anaemia and Vitamin D Deficiency
Authors: Nasreen Shaikh Zinnat Ara , Mahjabeen Nusrat , Shahreen Safinaz 
Published: March 2023

Background: Postpartum depression (PPD) affects about 15% of mothers. It is a serious disorder that may affect physical and mental health of new mothers and newborns. Mothers suffering from anemia and vitamin D deficiency may be at increased risk of developing PPD.
Aim: Our study aimed to detect the association of anaemia and Vit D deficiency with postpartum depression & also to look at association of other maternal and perinatal complications.
Materials & methods: In this cross sectional observational study, pregnant women having anemia (Hb% <11gm/dl), were grouped as A (n=141), pregnant women having low Vit D were grouped as B (n=28), and pregnant women with normal Hb% and normal Vit D were grouped as grouped C (n=58). A & B were taken as cases and group C was taken as control in the study. Depression was evaluated by using Edinburg postnatal depression rating scale, P <0.05 was taken as statistically significant. Also secondary outcome like PPH, wound infection, Apgar score, preterm birth neonatal admission were noted down.
Results: Depression was found significantly higher in both A= 60.3% and B =14.2%, than group C =0% (p<0.0001). Postpartum hemorrhage (PPH) and wound infection were found higher for group A (58% & 13%), Group B (28 &3%) respectively than control group C (5 &2%). Interestingly all women with VitD deficiency had postpartal haemorrhage (PPH). Regarding neonatal outcome, APGAR score was low in A (38%) and B (10.7%) in compare to control group C (0%). NICU Admission was seen higher in A=26% & B=10.7% than for C=0%. Incidence of Preterm birth (PTB) for A =25% & B= 2% were significantly higher than group C= 0%. No stillbirth was found in any group.
Conclusion: Prevention, identification and treatment of anaemia and Vitamin D deficiency in pregnant women seem necessary, as they are strongly associated with postpartum depression and other complications.
J Bangladesh Coll Phys Surg 2023; 41: 114-119
Impact of Pregnancy Induced Hypertension on Babies
Authors: Sirajum Monira  , Mosammat Afroza Jinnat  , Md Tafazzal Hossain Khan  , M A Salim  , Mashura Musharraf  
Published: March 2023

Background: Pregnancy-induced hypertension (PIH) is a relatively common pregnancy disorder that causes variable maternal and fetal problems. This may increase the number of perinatal asphyxia, sepsis, still birth etc. It also has a significant affect on birth weight which is also related to gestational age. Objectives: To observe the impact of pregnancy induced hypertension on their newborn babies and compare the findings with the babies of normotensive mother. Materias and Methods: This case control study was conducted from July 2016 to December 2016. The study group was selected from the babies of pregnancy induced hypertension (PIH) mothers and control group selected from babies of a normotensive mother attending in Obstetrics department of Dhaka Medical College Hospital, Dhaka having the inclusion and exclusion criteria. The total number of 100 neonates were included consecutively in this study. 50 neonates born to mothers with pregnancy-induced hypertension were grouped as cases and 50 neonates born to normotensive mothers were grouped as control. Results: The study revealed that, the highest frequency of neonatal morbidities were perinatal asphyxia (22%) among the babies of PIH mother. Stillbirth it was found 12 % . The duration of pregnancy were also affected by PIH where most of the babies were preterm (88%) and complete-term pregnancy found only (12%). It should be mentioned that those mother having history of preeclampsia delivered more premature babies (p <0.001). Another important outcome birth weight related to gestational age was also very important which were measured as the highest for SGA 92% and AGA 8%. The weight of the baby were significantly (p<0.001) reduced in those who had a history of PIH and the mean weight was 1592.78 ± 470.06. Conclusion: Prematurity, Small for gestational-age babies and perinatal asphyxia are more common in Pregnancy induced hypertension group than in the normotensive group.
KYAMC Journal Vol. 13, No. 04, January 2023: 204-207
Obesity and Its Co-Morbidities among School Teachers in Dhaka City
Authors: Ripon Barua  , Imranul  Mawa  , Rajib Chowdhury  , Muhammad Alamgir Mandal  
Published: March 2023

Background: Obesity is an important cause for human morbidity and mortality. Objective: This study was aimed to determine the prevalence of obesity and its co-morbidities among school teachers in Dhaka city. Materials and Methods: This cross sectional study was carried out from May 2017 to October of 2017. Total 250 school teachers aged between 25-59 years were enrolled using cluster random sampling technique. Data on socio-demographic profile, co-morbidities, weight, height and blood pressure were recorded. Results: The prevalence of obesity among school teachers was 15.2%. The mean age of the participants was 46.1±8.2 years. Female teachers were more obese compared to male (20% vs 5.9%), this association was statistically significant (p=0.001). Majority obese participants belonging to the age group of 41-59 years with higher prevalence among female compared to male (25.2% vs 5.8%), this was statistically significant (p=0.030). About 53% obese teachers had hypertension (HTN). On the other hand, Pre-HTN was observed more among obese participants (42.1%) compared to overweight (14.8%). About one third of overweight and obese participants were diabetic (30.6% and 34.2% respectively) and dyslipidaemic (21.5% and 23.7% respectively). Conclusion: The prevalence of obesity and its co-morbidities such as HTN, diabetes mellitus (DM), dyslipidaemia etc was high among the study participants. By promoting healthy life style and behaviors can reduce the burden of obesity and its co-morbidities.
KYAMC Journal Vol. 13, No. 04, January 2023: 245-249
PENGEMBANGAN MEDIA DASI (DIORAMA SIKLUS AIR) UNTUK MENINGKATKAN PEMAHAMAN KONSEP SISWA KELAS V SD
Authors: Yanti Yulia Eka , Huda Miftahul 
Published: March 2023

Penelitian ini bertujuan untuk mengembangkan sebuah produk media pembelajaran yang diberi nama DASI (Diorama Siklus Air) agar pemahaman konsep siswa kelas V pada materi siklus air tema 8 subtema 1 pembelajaran 2. Jenis penelitian yang digunakan adalah penelitian Research and Development (R&D). Adapun model R&D yang dipakai adalah model 4D (Thiagarajan). Pengumpulan data menggunakan instrumen penelitian yang diperlukan meliputi: wawancara, tes pemahaman konsep, angket, dan dokumentasi. Hasil penelitian menunjukkan bahwa 1) media DASI (Diorama Siklus Air) dinyatakan layak digunakan berdasarkan hasil validasi ahli media mendapatkan persentase akhir sebesar 100% dengan kualifikasi valid dan hasil validasi ahli materi mendapatkan persentase akhir sebesar 86% dengan kualifikasi valid. Sedangkan angket respon siswa memperoleh persentase akhir sebesar 89% dengan kualifikasi sangat layak dan 88% dengan kualifikasi sangat layak pada kelompok besar. 2) media pembelajaran DASI (Diorama Siklus Air) dinyatakan efektif untuk meningkatkan pemahaman konsep siswa berdasarkan skor rata-rata pretest kelompok kecil sebesar 54 dan skor rata-rata posttest sebesar 76 sedangkan ketuntasan klasikal pretest sebesar 40% dan klasikal sebesar 80% dengan peningkatan hasil belajar sebesar 40%. Sedangkan skor rata-rata pretest kelompok kecil sebesar 48 dan skor rata-rata posttest sebesar 74,5 sedangkan ketuntasan klasikal pretest sebesar 15% dan ketuntasan klasikal posttest sebesar 75% dengan peningkatan hasil belajar sebesar 60%.
Reflexão sobre o tempo de tela e a qualidade do sono
Authors: Figueredo Jaqueline Gonçalves Larrea , Frozza Marjorie Fernanda Rocha , Alencar Telma Soares de , Souza José Carlos Rosa Pires de , Machado Alessandra Aparecida Vieira , Cunha Lucas Rasi 
Published: March 2023

A luz azul-violeta é emitida por diversos equipamentos eletrônicos tais como computadores, tablets e smartphones. Essa luz estimula várias regiões cerebrais e interfere no funcionamento natural do organismo. Objetivos: avaliar as consequências do tempo de tela na qualidade do sono dos estudantes da escola estadual João de Paula Ribeiro. Metodologia: o presente artigo se trata de um estudo quantitativo, realizado por meio da aplicação de três questionários online entre os estudantes. O primeiro questionário foi elaborado pelos autores e contém variáveis sociodemográficas. Para a identificação da qualidade do sono entre os estudantes, foi aplicado o Índice de Qualidade do Sono de Pittsburgh (PSQI). Os responsáveis por cada estudante incluído na pesquisa autorizaram a participação destes por meio do preenchimento do Termo de Consentimento Livre e Esclarecido. Resultados: dos 57 estudantes que participaram da pesquisa, 33,3% passam mais do que 6h diante da tela (tablet, celular, computador, televisão, entre outros). Embora apenas 19,3% dos estudantes tenham classificado sua qualidade de sono como “ruim” ou “muito ruim”, cerca de 26% tiveram dificuldade de dormir porque não conseguiu adormecer em 30 minutos, na frequência de três ou mais vezes na semana. Conclusão: o tempo maior de exposição às telas leva a maiores prejuízos nos aspectos relacionados ao sono, com distúrbios como a insônia e a sonolência diurna excessiva; estes, por conseguinte, levam a sérios comprometimentos cognitivos e acadêmicos.
Aspectos fisiopatológicos da Síndrome de Sjögren: uma revisão narrativa
Authors: Batista Matheus Neres , Ferreira Gabrielly Rodrigues , Costa Angela Maria Pereira da , Miranda Gabriela de Deus , Lopes Amanda Xavier , Pereira Thays Inocencio , Ramalho Annelysa Vitoria Souza , Lopes Bárbara Xavier , Melo Rhaissa Vasconcelos , Dalmagro Maria Eduarda Tres 
Published: March 2023

A Síndrome de Sjogren (SS) é uma doença autoimune sistêmica caracterizada por acometimento de glândulas exócrinas, além de presença de sinais/sintomas de doença sistêmica. As glândulas lacrimais e salivares são frequentemente afetadas, causando a síndrome sicca, caracterizada por ressecamento dos olhos (xeroftalmia) e da boca (xerostomia). O presente artigo tem como objetivo abordar uma linha fisiopatológica da SS em contexto clínico. Este estudo trata-se de uma revisão narrativa com o propósito de discutir e descrever sobre a fisiopatologia da SS em seu contexto clínico. Foi utilizado o banco de dados: SciElo (Scientific Eletronic Library Online) e PubMed (US National Library of Medicine), com restrição de idioma (português) e dando prioridade a artigos publicados entre 2010 e 2022. A infiltração de células mononucleares, fatores humorais como anticorpos e citocinas, são descritos pela literatura os responsáveis por causar uma alteração estrutural e consequentemente funcional da glândula exócrina, causando uma acentuada diminuição da produção de lágrimas pelas glândulas lacrimais e diminuição da produção de saliva pelas glândulas salivares. Em suma, a SS continua sendo um fardo significativo para os sistemas de saúde em todo o mundo. Porém, os avanços feitos na compreensão de sua fisiopatologia nos últimos 20 anos superam tudo o que foi feito antes.
Jauss e Iser: efeitos estéticos provocados pela leitura de Conversa de Bois e Campo Geral, de João Guimarães Rosa
Authors: Sirino Salete Paulina Machado , Fortes Rita das Graças Felix 
Published: March 2023

Partindo da premissa de que a práxis da leitura literária é uma inesgotávelfonte de pesquisa, este estudo tem como fator norteador o exercí­cio de leituraanalí­tico interpretativa, do conto Conversa de Bois e da novela Campo Geral/Miguilim– publicados respectivamente em 1946 e 1956 –, de João Guimarães Rosa, aliado í steorias da recepção de Hans Robert Jauss (1979) e Wolfgang Iser (1979). Tal análiseobjetiva, especialmente, a leitura da temática infância presentes nos referidos textosrosianos.Palavras"chave: Conversa de Bois e Campo Geral. Teorias da Recepção. Infância.
Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts
Authors: García-García Óscar Darío , El Soury Marwa , Campos Fernando , Sánchez-Porras David , Geuna Stefano , Alaminos Miguel , Gambarotta Giovanna , Chato-Astrain Jesús , Raimondo Stefania , Carriel Víctor 
Published: March 2023

As a reliable alternative to autografts, decellularized peripheral nerve allografts (DPNAs) should mimic the complex microstructure of native nerves and be immunogenically compatible. Nevertheless, there is a current lack of decellularization methods able to remove peripheral nerve cells without significantly altering the nerve extracellular matrix (ECM). The aims of this study are firstly to characterize ex vivo, in a histological, biochemical, biomechanical and ultrastructural way, three novel chemical-enzymatic decellularization protocols (P1, P2 and P3) in rat sciatic nerves and compared with the Sondell classic decellularization method and then, to select the most promising DPNAs to be tested in vivo. All the DPNAs generated present an efficient removal of the cellular material and myelin, while preserving the laminin and collagen network of the ECM (except P3) and were free from any significant alterations in the biomechanical parameters and biocompatibility properties. Then, P1 and P2 were selected to evaluate their regenerative effectivity and were compared with Sondell and autograft techniques in an in vivo model of sciatic defect with a 10-mm gap, after 15 weeks of follow-up. All study groups showed a partial motor and sensory recovery that were in correlation with the histological, histomorphometrical and ultrastructural analyses of nerve regeneration, being P2 the protocol showing the most similar results to the autograft control group.
Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
Authors: Zhang Yingying , Luo Xi , Yin Longfei , Yin Fengwei , Zheng Weilong , Fu Yongqian 
Published: March 2023

Chitosan is a biopolymer material extracted from marine biomass waste such as shrimp and crab shells, which has good biocompatibility and degradability with great potential for application in the field of wastewater treatment and soil remediation. The higher the degree of deacetylation (DD), the better the adsorption performance of chitosan. Chitin deacetylase (CDA) can specifically catalyze the deacetylate of chitin in a green reaction that is environmentally friendly. However, the scarcity of high yielding chitin deacetylase strains has been regarded as the technical bottleneck of chitosan green production. Here, we screened a natural chitin degrading bacterium from coastal mud and identified it as Bacillus cereus ZWT-08 by re-screening the chitin deacetylase activity and degree of deacetylation values. By optimizing the medium conditions and enzyme production process, ZWT-08 was cultured in fermentation medium with 1% (m/V) glucose and yeast extract at pH 6.0, 37°C, and a stirring speed of 180 r/min. After fermenting in 5 L fermenter for 48 h, the deacetylation activity of the supernatant reached 613.25 U/mL. Electron microscopic examination of the chitin substrate in the fermentation medium revealed a marshmallow-like fluffy texture on its structural surface. Meanwhile, 89.29% of the acetyl groups in this chitin substrate were removed by enzymatic digestion of chitin deacetylase produced by ZWT-08, resulting in the preparation of chitosan a degree of deacetylation higher than 90%. As an effective strain for chitosan production, Bacillus cereus ZWT-08 plays a positive role in the bioconversion of chitin and the upgrading of the chitosan industry.
Multimodality imaging to identify lipid-rich coronary plaques and predict periprocedural myocardial injury: Association between near-infrared spectroscopy and coronary computed tomography angiography
Authors: Ota Hideaki , Matsuo Hitoshi , Imai Shunsuke , Nakashima Yuki , Kawase Yoshiaki , Okubo Munenori , Takahashi Hiroshi , Kawai Hideki , Sobue Yoshihiro , Kawasaki Masanori , Kondo Takeshi , Muramatsu Takashi , Izawa Hideo 
Published: March 2023

BackgroundThis study compares the efficacy of coronary computed tomography angiography (CCTA) and near-infrared spectroscopy intravascular ultrasound (NIRS–IVUS) in patients with significant coronary stenosis for predicting periprocedural myocardial injury during percutaneous coronary intervention (PCI).MethodsWe prospectively enrolled 107 patients who underwent CCTA before PCI and performed NIRS–IVUS during PCI. Based on the maximal lipid core burden index for any 4-mm longitudinal segments (maxLCBI4mm) in the culprit lesion, we divided the patients into two groups: lipid-rich plaque (LRP) group (maxLCBI4mm ≥ 400; n = 48) and no-LRP group (maxLCBI4mm &lt; 400; n = 59). Periprocedural myocardial injury was a postprocedural cardiac troponin T (cTnT) elevation of ≥5 times the upper limit of normal.ResultsThe LRP group had a significantly higher cTnT (p = 0.026), lower CT density (p &lt; 0.001), larger percentage atheroma volume (PAV) by NIRS–IVUS (p = 0.036), and larger remodeling index measured by both CCTA (p = 0.020) and NIRS–IVUS (p &lt; 0.001). A significant negative linear correlation was found between maxLCBI4mm and CT density (rho = −0.552, p &lt; 0.001). Multivariable logistic regression analysis identified maxLCBI4mm [odds ratio (OR): 1.006, p = 0.003] and PAV (OR: 1.125, p = 0.014) as independent predictors of periprocedural myocardial injury, while CT density was not an independent predictor (OR: 0.991, p = 0.22).ConclusionCCTA and NIRS–IVUS correlated well to identify LRP in culprit lesions. However, NIRS–IVUS was more competent in predicting the risk of periprocedural myocardial injury.
Injury to the tunica media initiates atherogenesis in the presence of hyperlipidemia
Authors: Belhoul-Fakir Hanane , Wu Jiansha , Yeow Yen L. , Musk Gabrielle C. , Kershaw Helen , Ingley Evan , Zhao Bichen Sophie , Reid Christopher M. , Lagat Christopher , Evans Brian , Thompson Peter L. , Brown Michael L. , Hamzah Juliana , Jansen Shirley 
Published: March 2023

Background and aimsFatty streaks initiating the formation of atheromatous plaque appear in the tunica intima. The tunica media is not known to be a nidus for lipid accumulation initiating atherogenesis. We assessed changes to the tunica media in response to a micro-injury produced in the pig aorta. In addition, we assessed human carotid endarterectomy plaques for indication of atheroma initiation in the tunica media.MethodsThree healthy landrace female pigs underwent laparotomy to inject autologous blood and create micro-hematomas at 6 sites within the tunica media of the infrarenal abdominal aorta. These pigs were fed a high-fat diet (HFD) for 4–12 weeks. Post-mortem aortas from all pigs, including a control group of healthy pigs, were serially stained to detect lipid deposits, vasa vasora (VV), immune cell infiltration and inflammatory markers, as well as changes to the vascular smooth muscle cell (vSMC) compartment. Moreover, 25 human carotid endarterectomy (CEA) specimens were evaluated for their lipid composition in the tunica media and intima.ResultsHigh lipid clusters, VV density, and immune cell infiltrates were consistently observed at 5 out of 6 injection sites under prolonged hyperlipidemia. The hyperlipidemic diet also affected the vSMC compartment in the tunica media adjacent to the tunica adventitia, which correlated with VV invasion and immune cell infiltration. Analysis of human carotid specimens post-CEA indicated that 32% of patients had significantly greater atheroma in the tunica media than in the arterial intima.ConclusionThe arterial intima is not the only site for atherosclerosis initiation. We show that injury to the media can trigger atherogenesis.