bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role and function of secretory protein MMP3 in cervical cancer
Authors: Shao Lei , Wang Xiaojuan , Liu Wanqiu , Zhang Chunyan , Ma Wei , Yu Xiao , Han Jing 
Published: February 2023

Abstract
OBJECTIVE
Cervical cancer(CCA) is the second commonest malignancy among female all over the world, and present clinical treatments cannot solve the problems of high metastasis and chemotherapy-resistant in CCA. This study starts with RNA-seq analysis and aims to investigate the possibility of secretory protein MMP3 as a new diagnosis and therapeutic target in CCA.
METHODS
Through conjoint analysis of gene expression data as well as survival rate data, we explored the potential secretary proteins associated with CCA carcinogenesis and advance and verify the expression changes in serum of clinical patients. We knockdown or overexpress the secretory proteins then explore its influence on biological function of CCA cells. Cell viabilities and apoptosis levels are detected using CCK-8 kit and TUNEL staining assay respectively, the expression of apoptosis related proteins was verified by western blot.
RESULTS
By cross-analysis of The Cancer Genome Atlas (TCGA) database and MetazSecKB database, MMP3 gene was most significantly upregulated in CCA patients. Also, MMP3 protein was remarkably increased in the serum of clinical CCA patients and decreased after receiving treatment. Overexpression of MMP3 in HT-3 cells or culturing new cells using the supernatant of the medium after MMP3 overexpression could increase cell viability (p < 0.05) as well as proliferation (p < 0.05). What’s more, knockdown of MMP3 reduced the phosphorylation of PI3K as well as AKT proteins, while the PI3K phosphorylation inhibitors could suppress the impact of MMP3 on increasing cell proliferation as well as viability.
Conclusion
The secreted protein MMP3 is significantly related to the development and progression of CCA in clinical, and MMP3 can inhibit apoptosis of CCA cells through regulating PI3K/Akt signal pathway. Therefore, this study suggests that MMP3 could be an underlying target for early diagnosis, together wo and treatment of CCA in the future.
Vitamin B12 deficiency-related glossitis is highly associated with serum high gastrin-17 and low pepsinogen I
Authors: Zhu Jingci , Feng Huang , He Yining , Wang Yufeng , Ge Zili 
Published: February 2023

Abstract
Background

To investigate the correlation between vitamin B12 deficiency-related glossitis (B12-def glossitis) and gastric serum biomarkers [gastrin-17(G17), pepsinogen I (PGI), pepsinogen II (PGII), and anti-H. pylori antibodies] and to analyze the association between B12 deficiency and gastric mucosal function and status.

Methods

A total of 148 patients diagnosed with B12-def glossitis were enrolled in the case group. Another 110 patients without manifestations of glossitis and B12-def were in the control group. General information, medical history, oral mucosa examination, and hematological results were extracted from patients’ medical records. The gastric serum biomarkers were compared between the case and control groups. Based on the oral lesions, case group patients were divided into atrophic or erythematous types, and hematological results were compared between the two groups. Subgroup analysis was also performed according to the results of gastric serum biomarkers in the case group to compare the degree of B12 deficiency in different subgroups and the effects on erythrocytes and oral mucosa.

Results

Of the 133 patients in the case group who underwent gastric serological testing, 112 (84.6%) showed serum high G17 and low PGⅠ levels (hereafter referred to as G17hi PGⅠlow), compared with 6.4% in the control group and the difference between the two groups was statistically significant. Hemoglobin (HB) concentration and red blood cell (RBC) amount were more likely to decrease in atrophic type, while the mean corpuscular volume (MCV) increased. Low serum B12 concentration, high serum folate concentration, and low prevalence of H. pylori infection were found in the G17hi PGⅠlow group and the gastrectomy group, all with statistically significant differences compared with those of the non- G17hi PGⅠlow group.

Conclusion

Gastric serum biomarkers in patients with B12-def glossitis generally showed G17hi PGⅠlow, suggesting possible atrophy of gastric corpus mucosa. The G17hi PGⅠlow and non- G17hi PGⅠlow groups may represent two or more different etiologies of B12 malabsorption. Patients with the erythematous form are probably in the early stages of B12 deficiency.
SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair
Authors: Wen Lu , Huang Yujia , Tang ming , Hu Zhiyi , Cai Bailian , Chen Guofang , Jiang Lijun , Xia Yan , Guan Pujun , Li Xiaoqi , Mao Zhiyong , Wan Xiaoping 
Published: February 2023

Abstract
Endometrial cancer (EC) is one of the most common malignant tumors of female genital tract with rapid increased incidence worldwide. Histone methyltransferase SMYD3, which is linked to the development and progression of various cancers, however, its oncogenic function in endometrial cancer has not been investigated thus far. In the present study, we report that the expression level of SMYD3 is significantly upregulated in EC samples and associated with EC progression. In vivo and in vitro functional experiments demonstrate that SMYD3 depletion inhibits cell proliferation, migration, and invasion abilities, increasing the sensitivity of EC cells to radiation. Moreover, with pathway enrichment analysis, we find that the DNA damage repair pathway is mainly involved in regulating EC progression. Mechanically, in response to DNA damage, SMYD3 is recruited to DNA damage sites in an PARP1-dependent manner and specifically methylates LIG4. Methylated LIG4 sequentially assembles LIG4/XRCC4/XLF complex and participates in non-homologous end joining repair (NHEJ) pathway. More importantly, pharmacological targeting of SMYD3 with its specific inhibitor BCI-121, significantly represses the tumorigenicity of EC cells and greatly enhanced radiotherapy efficiency. Overall, our data suggest SMYD3 as a pivotal factor in NHEJ repair and is associated with in EC progression. Using in vitro and in vivo system, we also prove that SMYD3 is a promising pharmacological target for endometrial cancer therapy.
Accurate Identification of Fly Species in Forensic, Medical and Veterinary Entomology using Wing Venation Patterns
Authors: Ling Min Hao , Ivorra Tania , Heo Chong Chin , Wardhana April Hari , Hall Martin Jonathan Richard , Tan Siew Hwa , Mohamed Zulqarnain , Khang Tsung Fei 
Published: February 2023

Abstract
In forensic, medical and veterinary entomology, the ease and affordability of image data acquisition have resulted in whole-image analysis becoming an invaluable approach for species identification. Krawtchouk moment invariants are a classical mathematical transformation that can extract local features from an image, thus allowing subtle species-specific biological variations to be accentuated for subsequent analyses. We extracted Krawtchouk moment invariant features from binarised wing images of 759 male fly specimens from the Calliphoridae, Sarcophagidae, and Muscidae families (13 species and a species variant). Subsequently, we trained the Generalized, Unbiased, Interaction Detection and Estimation (GUIDE) random forests classifier using linear discriminants derived from these features and inferred the species identity of specimens from the test samples. Five-fold cross validation results show a 98.56 ± 0.38% (standard error) identification accuracy at the family level, and a 91.04 ± 1.33% identification accuracy at the species level. The mean F1-score of 0.89 ± 0.02 reflects good balance of precision and recall properties of the model. The present study consolidates findings from previous small pilot studies of the usefulness of wing venation patterns for inferring species identities. Thus, the stage is set for the development of a mature data analytic ecosystem for routine computer image-based identification of fly species that are of forensic, medical, and veterinary importance.
Association between SGLT2 inhibitors and diabetic ketoacidosis among patients fasting in Ramadan
Authors: Ruqaib Abdullah M Al , Ghamdi Abdulrahman Aidh Al , Jamaan Abdulaziz Al , Abdulmowla Majd Muwafak , Deaji Fahad F Al , ALsuwaidan Salem 
Published: February 2023

Abstract
Aims: The use of SGLT2 inhibitors during Ramadan may be unsafe (risk of ketoacidosis, postural hypotension and dehydration), specifically during long hours of Ramadan fasting in hot climates. The objective of this study was to determine the main risk factors associated with the increased occurrence of DKA during Ramadan among patients utilising SGLT2 inhibitors and assess DKA risk during Ramadan.
Methods: This retrospective diagnostic study assessed the role of SGLT2 inhibitors in 99 patients (50 men and 49 women) with declared diabetes mellitus treated with empagliflozin and followed-up in diabetes clinics. The main variables were:
1. Demographic data (age and gender).
2. Number of break days during Ramadan fasting.
3. Comorbidities associated with diabetes.
4. Signs and symptoms of DKA.
Most of the patients (61 subjects) had a 6–10 year diabetes history, and 93 patients continued their regular follow-up. About 93% of the patients were used to Ramadan fasting, whereas only five patients did not fast in Ramadan.
Results: Thirty-one of the fasting patients broke fasting during Ramadan for 1–5 days, and only two patients did so for more than 6 days. Patients exhibited known diabetes mellitus complications, such as hypertension, dyslipidaemia, cardiovascular and other associated diseases. None of the participants showed diabetic ketoacidosis signs and symptoms.
Conclusions: SGLT2 inhibitors are considered an effective antidiabetic agent that can be safely used in patients with diabetes who are fasting in Ramadan.
Comparison of student performance on the pre-internship medical qualification examination at Debre Tabor University, Ethiopia
Authors: Belay Lalem Menber , Melkamu Yewulsew Assaye , Wassie Fentahun Dires 
Published: February 2023

Abstract
Introduction: Competency assessment has a substantial impact on both the quality of education and the quality of care. Debre Tabor University has been administering pre-internship qualification exams to ensure clinical competence and readiness for internship practice. A variety of assessment methods, including MCQs, objective structured clinical exams, and external exams were used. The reliability of results can be determined by how consistently students perform across different assessment formats.
Objective: The objective of this study was to compare the scores obtained on the different assessment formats used in the pre-internship qualification examination.
Methods: A correlational study was conducted at the College of Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia from November to December 2019. Participants of the study were medical students who completed their fifth-year medical education. All 46 fifth-year medical students who took the qualifying exam were included in the analysis. The data were analyzed using descriptive statistics such as percentage and mean as well as inferential statistics like Pearson's correlation. The analysis was done with STATA software.
Results: The mean performance score ranged from 67.9 on multiple-choice questions to 77.2 on viva. Relatively, students performed better on the viva test and poorly on the OSCE. There was no significant difference in the overall performance of male and female students, however, female students were better in long- and short-case exams. For female students, the correlation between performance scores was stronger. When compared to their written exam results, female students performed much better on the long-case, short-case, and oral exams, but men did not show such a difference.
Conclusions: Students performed better on the oral examination. The study demonstrated a disparity between female and male students on the level of performance in the clinical skills exams. Among female students, there was a substantial correlation between cognitive ability and clinical performance. Gender was an effect modifier for the correlation between cognitive ability and clinical skill performance in the medical qualification examination.
Combining graph convolutional networks and Chebyshev polynomial approximation of spectral filter to improve spectral clustering
Authors: Ni Zhongyuan , Liu Jinglei 
Published: February 2023

Abstract
Spectral clustering(SC) is a widely used method of unsupervised learning, which is effective in dealing with many real-world applications. However, existing SC simply drops in three deficiencies. Firstly, the traditional SC cannot learn an effective representation among non-Euclidean data. Secondly, for the present algorithms based on spectral clustering, it is necessary to carry out eigendecomposition of the Laplace matrix, that is very time consuming when processing large-scale data. Thirdly, in large-scale data, the application of the k-means algorithm for spectral clustering will also waste time. To solve these issues, we combine Graph Convolutional Network and Chebyshev polynomial approximation of spectral filter to improve spectral clustering (CGCSC). Specifically, the proposed CGCSC algorithm first uses Graph Convolutional Networks (GCN) to express and analyze non-Euclidean data, and then builds the Laplace matrix. Meanwhile,we use Chebyshev polynomial approximation of spectral filter technology to avoid the eigendecomposition of the Laplace matrix, which is completely different from traditional ways. Additionally, this paper adopts a uniform distribution sampling technique, which can do a random sample of graph signals, further reduce the data scale and improve the k-means’speed in SC. The performance of CGCSC is tested on artificial and natural datasets. Compared with several advanced clustering algorithms, the experiment shows that CGCSC can considerably improve the clustering speed, and at the same time ensuring the clustering accuracy.
PRMT5-exacerbating tumor stemness and immunosuppression confer a novel immunotherapeutic vulnerability in aggressive prostate cancer
Authors: Zou Cheng , Xu Bin , Li Wenchao , He Qinju , Liu Ruifang , Zhang Yuanzhen , He Lingjing , Tracz Amanda , Zhao Ruizhe , Yuan Kai , Hu Qiang , Liu Song , Gao Guozhen , Bedford Mark , Tang Dean , Zhang Dingxiao 
Published: February 2023

Abstract
Protein arginine methylation is a common post-translational modification (PTM) catalyzed by nine protein arginine methyltransferases (PRMTs). As the major symmetric arginine methyltransferase that methylates both histone and non-histone substrates, PRMT5 plays key roles in a number of biological processes critical for development and tumorigenesis. PRMT5 overexpression has been reported in multiple cancer types including prostate cancer (PCa), but the exact biological and mechanistic understanding of PRMT5 in aggressive PCa remains ill-defined. Here, we show that PRMT5 is upregulated in PCa, correlates with worse patient survival, promotes corrupted RNA splicing, and functionally cooperates with an array of pro-tumorigenic pathways to enhance oncogenesis. PRMT5 inhibition via either genetic knockdown or pharmacological inhibition reduces stemness with paralleled differentiation and arrests cell cycle progression without causing appreciable apoptosis. Strikingly, the severity of antitumor effect of PRMT5 inhibition correlates with disease aggressiveness, with AR+ PCa being less affected. Molecular characterization pinpoints MYC, but not (or at least to a lesser degree) AR, as the main partner of PRMT5 to form a positive feedback loop to exacerbate malignancy in both AR+ and AR- PCa cells. Inspired by the surprising finding that PRMT5 negatively correlates with tumor immune infiltration and transcriptionally suppresses an immune-gene program, we further show that although PRMT5 inhibitor (PRMT5i) EPZ015666 or anti-PD-1 immunotherapy alone exhibits limited antitumor effects, combination of PRMT5i with anti-PD-1 displays superior efficacy in inhibiting castration-resistant PCa (CRPC) in vivo. Finally, to expand the potential use of PRMT5i through a synthetic lethality concept, we also perform a global CRISPR/Cas9 knockout screen to unravel that many clinical-grade drugs of known oncogenic pathways can be repurposed to target CRPC when used in combination with PRMT5i at low doses. Collectively, our findings establish a rationale to exploit PRMT5i in combination with immunotherapy or other targeted therapies to treat aggressive PCa.
Investigating the effect of mixed probiotics on the hippocampal tissue and stress hormone levels and BDNF gene expression in the first generation offsprings of male mice whose mothers received chronic immobility stress during pregnancy
Authors: azimian hosein akbar , navaeian majid , pakpour bahareh 
Published: February 2023

Abstract
Introduction: simply put, probiotics are beneficial bacteria for our body and play an important role in chronic stress. They have an effect on the behavior of creatures and are transmitted from mother to child during pregnancy. New research has confirmed the effect of probiotics on reducing stress. This study aimed to assess the effects of probiotics on pregnancy stress in first generation off springs in mice.
Method: In this experimental study, 24 mice with a weight range of 28 to 38 g were identified and placed in 3 groups; normal, water, PRB(probiotic ). The probiotic group received immobility stress with probiotic gavage during pregnancy. The water group received saline-induced immobility stress during pregnancy, and the normal group received none stress and gavage. Each group consisted of 8 male offsprings. The elevated plus maze and hole board tests were carried out. The levels of BDNF gene expression in the brain of mice were measured. Serum corticosterone and ACTH levels were measured, then the hippocampus tissue in mice was examined using hematoxylin and eosin staining.
Results: Behavioral tests showed that the open arm time among offsprings whose mothers had received probiotics during stress was greater than the other groups, which indicated a lower level of stress. Measurement of corticosterone and ACTH hormones in the serum of first generation neonates showed that the amount of hormones in the probiotic group was significantly reduced compared to the other two groups. The level of BDNF gene expression in the probiotic group was higher than in the water group and the number of dead hippocampal cells in the brain of the pro biotic group mice was less than the number of dead cells in the hippocampus of the water group mice.
Discuss: Consumption of probiotics during pregnancy can reduce stress in first generation children, which was proven by behavioral tests and hormonal and tissue tests in the present study. Probiotics with antioxidant roles seem to reduce cellular inflammation and, by absorbing a variety of vitamins and neurotransmitter precursors, they play an important role in reducing stress in first generation off springs.
Clinical testing of transcriptome-wide expression profiles in high-risk localized and metastatic prostate cancer starting androgen deprivation therapy: an ancillary study of the STAMPEDE abiraterone Phase 3 trial
Authors: Attard Gerhardt , Parry Marina , Grist Emily , Mendes Larissa , Dutey-Magni Peter , Sachdeva Ashwin , Brawley Christopher , Murphy Laura , Proudfoot James , Lall Sharanpreet , Liu Yang , Friedrich Stefanie , Ismail Mazlina , Hoyle Alex , Ali Adnan , Haran Aine , Wingate Anna , Zakka Leila , Wetterskog Daniel , Amos Claire , Atako Nafisah , Wang Victoria , Rush Hannah , Jones Robert , Leung Hing , Cross William , gillessen silke , Parker Chris , Chowdhury Simon , Lotan Tamara , Marafioti Teresa , Urbanucci Alfonso , Schaeffer Edward , Spratt Daniel , Waugh David , Powles Thomas , Berney Daniel , Sydes Matthew , Parmar Mahesh , Hamid Anis , Feng Felix , Sweeney Christopher , Davicioni Elai , Clarke Noel , James Nicholas , Brown Louise 
Published: February 2023

Abstract
Metastatic and high-risk localized prostate cancer respond to hormone therapy but outcomes vary. Following a pre-specified statistical plan, we used Cox models adjusted for clinical variables to test associations with survival of multi-gene expression-based classifiers from 781 patients randomized to androgen deprivation with or without abiraterone in the STAMPEDE trial. Decipher score was strongly prognostic (p<2x10-5) and identified clinically-relevant differences in absolute benefit, especially for localized cancers. In metastatic disease, classifiers of proliferation, PTEN or TP53 loss and treatment-persistent cells were prognostic. In localized disease, androgen receptor activity was protective whilst interferon signaling (that strongly associated with tumor lymphocyte infiltration) was detrimental. Post-Operative Radiation-Therapy Outcomes Score was prognostic in localized but not metastatic disease (interaction p=0.0001) suggesting the impact of tumor biology on clinical outcome is context-dependent on metastatic state. Transcriptome-wide testing has clinical utility for advanced prostate cancer and identified worse outcomes for localized cancers with tumor-promoting inflammation.
How climate policy reduces green financing risk
Authors: Zheng Huanhuan 
Published: February 2023

Abstract
The social impact of climate policy can be transformed into private benefit to green finance through attracting sustainable investors, who seek to increase the social impact of their green investments. Comparing green and conventional bonds issued before and after the adoption of climate policy, we show that climate policy strengthens corporate capacity to borrow foreign capital in local-currency green bonds. This uncovers a new role of climate policy in reducing an important source of international financing risk that challenges financial stability: the currency mismatch risk arising from borrowing in foreign currency while earning in local currency. In this vein, climate policy is an analogy of credible monetary policy for green finance. The role of climate policy in reducing currency mismatch risk is more pronounced for firms with higher ESG scores, richer international green financing experience, and from markets with better environmental performance.
JEL: G18, F34, Q54, Q58
Simulation of Pollutant Diffusion in Vegetation Open Channel Based on LBM-CA Method
Authors: Wang Shiyu , Zhuo Jialin , Jia Fengcong , Deng Liuhong , Wang Hongru , Han Yu 
Published: February 2023

Abstract
The aim of the present study was to accurately assess the changes in the degree of instantaneous pollution of natural river. In this paper, a pollutant diffusion model (LBM-CA) for vegetation open channel is constructed. This model consists partly of Cellular Automaton (CA), and the main task of this part is to simulate the pollutant diffusion. Flow influence coefficient is introduced into Cellular Automata to express the influence of vegetation on pollutant diffusion. In order to obtain the flow influence coefficients for each cellular, Lattice Boltzmann Method (LBM) is introduced in the LBM-CA model to simulate the flow in vegetation open channels. The results show that the LBM-CA model has high accuracy. The simulation results show that the existence of vegetation will lead to the accumulation of pollutants and prolong the residence time of pollutants in the vegetation area. The pollutant limits are embedded in the LBM-CA model to predict the pollution level of the basin at a specific time. LBM-CA model provides a method for simulating pollutants diffusion in natural rivers.