bluuebunny/crossref_metadata_2025_split huggingface dataset:
CREB1 spatio-temporal dynamics within the rat pineal gland
Authors: Farias Altamirano Luz E . , Vásquez Elena , Freites Carlos L. , Ibañez Jorge E. , Guido Mario E . , Muñoz Estela M . 
Published: September 2023

In the rat pineal gland (PG), cyclic AMP responsive element-binding protein 1 (CREB1) participates in the nocturnal melatonin synthesis that rhythmically modulates physiology and behavior. Phosphorylation of CREB1 is one of the key regulatory steps that drives pineal transcription. The spatio-temporal dynamics of CREB1 itself in the different PG cell types have not yet been documented. In this study we analyzed total CREB1 in the rat PG via Western blot and fluorescence immunohistochemistry followed by confocal laser-scanning microscopy and quantitative analysis. Total CREB1 levels remained constant in the PG throughout the light:dark cycle. The distribution pattern of nuclear CREB1 did vary among PG cell types. Pinealocytes emerged to have discrete CREB1 domains within their nucleoplasm that were especially distinct. The number, size, and location of CREB1 foci fluctuated among pinealocytes, within the same PG and among Zeitgeber times (ZTs). A significantly larger dispersion of CREB1-immunoreactive nuclear sites was found at night than during the day. However, the overall transcription activity was mostly conserved between the light and dark phases, as shown by the expression of a particular phosphorylated form of the RNA polymerase II (RNAPII-pSer5CTD). Suppression of the nocturnal norepinephrine pulse by chronic bilateral superior cervical ganglionectomy increased CREB1 dispersion in pinealocyte nuclei at early night, as compared to sham-derived cells. In addition, differences in CREB1 distribution were found between sham-operated and non-operated rats at ZT14. Together, these data suggest that in mature pinealocytes, nuclear CREB1 is subjected to a dynamic spatio-temporal distribution. Further studies are necessary to elucidate the underlying mechanisms and to understand the impact of CREB1 reorganization in the pineal transcriptome.
ROLE OF SUCTION/INJECTION ON MIXED CONVECTION FLOW IN A VERTICAL MICROCHANNEL FILLED WITH POROUS MATERIAL
Authors: Daramola Deborah A. , Ajibade Abiodun O. , Lawal Hammed. A. 
Published: September 2023

A suction/injection controlled mixed convection flow of an incompressible and viscous fluid in a vertical microchannel filled with porous material with asymmetric plate temperature is presented. The governing equations are derived and solved using the method of undetermined coefficient. The closed form expression for temperature field, velocity field, skin friction and Nusselt number for the steady fully developed flow are obtained analytically. The effects of the governing parameters on the microchannel hydrodynamic and thermal behaviors are determined. It is interesting to point out that growing the Darcy number as well as the suction S accelerate the fluid motion while they reduce the critical values of the ( at which flow reversal sets in ) on both plates. The stability of the flow is affected by variation in the permeability of the porous material.
PEMANFAATAN LIMBAH PLASTIK MENJADI PRODUK KREATIF SEBAGAI MEDIA PEMBELAJARAN BAGI PELAJAR DESA TERUSAN MULYA, KABUPATEN KAPUAS, KALIMANTAN TENGAH
Authors: Wibowo Dimas Mulianto , Sari Julia Purnama 
Published: September 2023

Semakin meningkatnya sampah di Desa Terusan Mulya ini akan menjadi masalah serius apabila tidak dicari penyelesaiannya. Di satu sisi penemuan plastik ini mempunyai dampak positif yang luar biasa, karena plastik memiliki keunggulan-keunggulan dibanding material lain. Berdasarkan analisis tersebut maka kegiatan pengabdian masyarakat ini perlu dilakukan dengan tujuan dapat meningkatkan kesadaran bagi para pelajar untuk peduli menjaga lingkungannya. Khususnya, mengelola limbah plastik menjadi produk kreatif atau kerajinan tangan yang dapat mengembangkan kreativitas serta rasa ingin tahu para pelajar.Berdasarkan hasil evaluasi diketahui, beberapa manfaat praktis yang diperoleh oleh para pelajar di Desa Terusan Mulya, yaitu: Para siswa/i mendapatkan informasi yang jelas dan utuh mengenai hakikat pemberdayaan masyarakat dari segi pengetahuan dan keterampilan; Para siswa/i yang menjadi peserta pelatihan memperoleh gambaran yang jelas mengenai potensi langkah pembuatan produk kreatif dengan memanfaatkan limbah sampah plastik sekali pakai untuk dapat dijual, sehingga bernilai ekonomis; Peserta pelatihan juga mendapatkan gambaran yang jelas dan utuh tentang manfaat limbah jika dikelola dengan baik.
Kata Kunci: Limbah Plastik, Produk Kreatif, Media Pembelajaran, terusan Mulya

ABSTRACT
THE USE OF PLASTIC WASTE INTO CREATIVE PRODUCTS AS A LEARNING MEDIA FOR STUDENTS IN TERUSAN VILLAGE, KAPUAS DISTRICT, CENTRAL KALIMANTAN. The increasing waste in the village of Terusan Mulya will become a serious problem if a solution is not sought. On the other hand, the discovery of plastic has had a tremendous positive impact, because plastic has advantages over other materials. Based on this analysis, this community service activity needs to be carried out with the aim of increasing awareness for students to care about protecting their environment. In particular, managing plastic waste into creative products or handicrafts can develop the creativity and curiosity of students. Based on the results of the evaluation, it is known that students in the Terusan Mulya Village have received several practical benefits, namely: Students get information clear and a complete understanding of the nature of community empowerment in terms of knowledge and skills; The students who become training participants get a clear picture of the potential steps for making creative products by utilizing single-use plastic waste to be sold, so it has economic value; Training participants also get a clear and complete picture of the benefits of waste if it is managed properly.
Keywords: Plastic Waste, Product Creative, Learning Media, Terusan Village
EKSPLORASI KECERDASAN BERHITUNG ANAK DENGAN PEMBELAJARAN TERBIMBING MELALUI PERMAINAN ABAKUS
Authors: Suratmin Idam Abdurrohim Hasani , Helmiyetti  
Published: September 2023

Anak adalah aset generasi bangsa. Di tangan merekalah estafet kehidupan dari generasi terdahulu akan diserahkan. Mendapat pendidikan yang layak adalah hak setiap anak. Namun banyak faktor yang mempengaruhi kualitas belajar anak. Pendidikan adalah salah satu upaya untuk mencerdaskan kehidupan bangsa. Sedangkan perlindungan anak merupakan suatu upaya untuk menciptakan kondisi dimana anak dapat melaksanakan hak dan kewajibanya. Berdasarkan hasil observasi yang telah dilakukan pada tanggal 03 s.d 12 Maret 2023 didapatkan hasil bahwa terdapat anak dengan inisialnya adalah RF yang tinggal di RT 13 memiliki keinginan belajar yang tinggi, terkhusus dalam bidang matematika. Namun antusias yang tinggi yang tidak didukung dengan lingkungan keluarga yang mendukung membuat anak tidak dapat mengembangkan pola belajar mereka terkait mata pelajaran matematika tersebut. Hal ini berakibat pada kemampuan anak dalam berhitung yang sangat buruk dan berdampak pada pendidikan anak tersebut di sekolah yang harus tidak naik kelas. Selain itu, permasalahan lain yang ditemui pada RF ialah kemampuan anak dalam mengutarakan pendapat di depan umum sangat kurang. Maka dari pelaksanaan Kuliah Kerja Nyata (KKN) kemudian menjadi suatu kesempatan untuk pengabdian dengan program mengadakan pendampingan belajar berhitung untuk mengeksplorasi kecerdasan RF dengan menggunakan media abakus. Hasil pendampingan diperoleh kemajuan kemampuan anak dalam memahami konsep perhitungan dasar (Penjumlahan, Pengurangan, Perkalian, dan Pembagian). Selain itu, kemampuan anak dalam berbicara di depan umum lebih terlihat dan anak lebih riang dari sebelum terjalannya program.
Asthma routinization, family asthma management, caregiver depressive symptoms, and medication adherence in Head Start preschool children
Authors: Lu Monica A. , Ruvalcaba Elizabeth , McQuaid Elizabeth L. , Rand Cynthia S. , Riekert Kristin A. , Eakin Michelle N. 
Published: September 2023

IntroductionMedication adherence is suboptimal in childhood asthma. Children rely on caregivers to manage medication administration. It is important to detect families who are at risk for poor adherence or to identify potential areas that can assist families with better adherence to asthma medications in order to improve asthma outcomes. We investigated the association between asthma routines, family asthma management knowledge and skills, and caregiver depressive symptoms with daily controller medication adherence among Head Start preschool children in Baltimore City.MethodsOur study included 256 low-income urban preschool children who were prescribed a daily controller medication. Asthma routinization (by the Asthma Routines Questionnaire), family asthma management [by the Family Asthma Management System Scale (FAMSS)], and caregiver depressive symptoms (by the Center for Epidemiological Studies – Depression) were assessed at baseline. The medication possession ratio (MPR) to measure adherence to daily controller medications was calculated at baseline and 12 months from pharmacy fill records. Multiple regression models evaluated the relationship between asthma routinization, the FAMSS, the CES-D, and MPR.ResultsResults indicated that only 7% of families had an MPR above 80% at baseline, and 24% of caregivers had clinically significant depressive symptoms. Higher asthma medication routines were associated with higher MPR at baseline (b = 0.05, p = 0.03). Higher family asthma management was associated with higher MPR at both baseline (b = 0.04, p &lt; 0.01) and 12 months (b = 0.05, p &lt; 0.01).DiscussionOur findings highlight the importance of family asthma management and maintaining medication routines over time to improve asthma controller medication adherence.
Comparative study on the international coal trade network before and after COVID-19 outbreak
Authors: Zhao Jingying , Huang Hui 
Published: September 2023

COVID-19 that broke out at the end of 2019 made countries to implement strict blockade measures to curb the spread of the virus. These measures have seriously disrupted the coal import and export trade, causing a significant impact on the coal trade network. This study aims to investigate the disparities between coal trade networks before and after COVID-19 outbreak. First, using complex network theory, we constructed global coal trade networks for the years 2019 and 2020. Subsequently, utilizing a cascading failure model, we developed a coal supply risk propagation framework. Through an analysis of network topology and theoretical supply risk propagation, we explored the similarities and differences of the coal trade networks during these 2 years. In terms of network topology structure, our findings reveal a decrease in trade tightness in the coal trade network of 2020 compared to 2019. Moreover, the Netherlands no longer retains its significance as an important trade hub country in 2020. Looking at the theoretical perspective of supply risk transmission, we observed that in 2020, COVID-19 resulted in a higher number of countries experiencing crisis outbreaks compared to 2019. However, it is noteworthy that the core countries within the coal trade network have remained consistent, and their roles as either net importers or net exporters have not changed. This indicates that the position of coal core countries is very stable. Therefore, no matter what happens, the country should pay timely attention to the dynamics of core countries. Interestingly, in 2020, the risk associated with coal supply did not conform to the spatial patterns typically seen in trade. For instance, the Russian Federation, identified as a country facing a crisis outbreak, transmits supply risks to geographically distant regions in West Asia, including countries like Ghana and Western Sahara.
Case Report: Complete pathological remission of human chorionic gonadotrophin-producing gallbladder carcinoma with multiple liver metastases after treatment with chemotherapy plus an immune checkpoint inhibitor
Authors: Wang Qianwen , Mu Yunchuan , Ji Shunxian , Liu Yang , Lou Yanbo , Wei Shumei , Dong Xin , Zhang Bo 
Published: September 2023

BackgroundGallbladder carcinoma (GBC) producing human chorionic gonadotrophin (HCG) is an extremely rare and highly invasive tumor with a poor prognosis. This unfavorable clinical outcome is partly due to the aggressive nature of the tumor and its insensitivity to chemotherapy.Case presentationWe herein report a case of primary GBC producing HCG with liver metastases in a 58-year-old woman. The patient presented with a markedly elevated β-HCG level and a mass in the gallbladder with multiple liver metastases. A definitive diagnosis was obtained after a needle biopsy of the liver metastases, showing poorly differentiated carcinoma with large-scale necrosis and strong positivity of immunostaining for HCG in tumor cells. The patient received chemotherapy (gemcitabine plus capecitabine) combined with carrellizumab, an immune checkpoint inhibitor (ICI). Pathological complete response was achieved after eight courses of combined therapy, which was confirmed by pathological analysis of resected specimens. After surgery, two courses of chemotherapy plus ICIs were adopted again. Complete response remained for approximately 1 year up to the present. Tumor tissue was collected to perform immunostaining of PD-L1, whole-exome sequencing, and RNA-seq. Low-TMB (1.51 mut/Mb), MSS, and high PD-L1 expression (TPS ≥ 50%) were observed in the tumor. Besides, the dominant types of infiltrating immune cells were macrophage and CD4+ T cells. Compared to other gallbladder adenocarcinoma without HCG, the proportion of M1 macrophage was at a higher level and the gene sets of MYC targets v1 and PI3K/AKT/mTOR signaling were highly expressed in our case. To the best of our knowledge, this is the first case report of complete remission of HCG-producing gallbladder carcinoma with liver metastases after chemotherapy combined with an immune checkpoint inhibitor. Furthermore, this is also the first report that described the tumor genetic feature and tumor immune microenvironment atlas of HCG-producing GBC.Conclusionchemotherapy plus an immune checkpoint inhibitor may provide a potentially curative option for gallbladder carcinoma with HCG production.
Case report: A novel CACNA1S mutation associated with hypokalemic periodic paralysis
Authors: Nuzhnyi Evgenii P. , Arestova Alina S. , Rossokhin Alexey V. , Protopopova Anna O. , Abramycheva Nataliya Yu , Suponeva Natalia A. , Illarioshkin Sergey N. 
Published: September 2023

BackgroundHypokalemic periodic paralysis (HypoKPP) is a rare neuromuscular genetic disorder causing recurrent episodes of flaccid paralysis. Most cases are associated with CACNA1S mutation, causing defect of calcium channel and subsequent impairment of muscle functions. Due to defined management approaches early diagnosis is crucial for promptly treatment and prevention new attacks.Materials and methodsWe report a case of HypoKPP associated with previously unreported mutation in CACNA1S gene (p.R900M). Molecular modeling of CaV1.1 was applied to evaluate its pathogenicity.ResultsAs a patient referred between attacks neurological status, laboratory and neurophysiological examination were unremarkable. Molecular modeling predicted that the p.R900M mutation affects the process of calcium channels activation.ConclusionNovel CACNA1S mutation, associated with HypoKPP was identified. Monte-Carlo energy minimization of the CaV1.1 model supported the association of this mutation with this disease.
Study of prevalence and risk factors of chemotherapy-induced mucositis in gastrointestinal cancer using machine learning models
Authors: Huang Lin , Ye Xianhui , Wu Fengqing , Wang Xiuyun , Qiu Meng 
Published: September 2023

ObjectiveChemotherapy-induced mucositis (CIM) significantly impacts clinical outcomes and diminishes the quality of life in patients with gastrointestinal cancer. This study aims to prospectively determine the incidence, severity, and underlying risk factors associated with CIM in this patient population.MethodsTo achieve this objective, we introduce a novel Machine Learning-based Toxicity Prediction Model (ML-TPM) designed to analyze the risk factors contributing to CIM development in gastrointestinal cancer patients. Within the winter season spanning from December 15th, 2018 to January 14th, 2019, we conducted in-person interviews with patients undergoing chemotherapy for gastrointestinal cancer. These interviews encompassed comprehensive questionnaires pertaining to patient demographics, CIM incidence, severity, and any supplementary prophylactic measures employed.ResultsThe study encompassed a cohort of 447 participating patients who provided complete questionnaire responses (100%). Of these, 328 patients (73.4%) reported experiencing CIM during the course of their treatment. Notably, CIM-induced complications led to treatment discontinuation in 14 patients (3%). The most frequently encountered CIM symptoms were diarrhea (41.6%), followed by nausea (37.8%), vomiting (25.1%), abdominal pain (21%), gastritis (10.5%), and oral pain (10.3%). Supplementary prophylaxis was administered to approximately 62% of the patients. The analysis revealed significant correlations between the overall incidence of CIM and gender (p=0.015), number of chemotherapy cycles exceeding one (p=0.039), utilization of platinum-based regimens (p=0.039), and administration of irinotecan (p=0.003). Specifically, the incidence of diarrhea exhibited positive correlations with prior surgical history (p=0.037), irinotecan treatment (p=0.021), and probiotics usage (p=0.035). Conversely, diarrhea incidence demonstrated an adverse correlation with platinum-based treatment (p=0.026).ConclusionIn conclusion, this study demonstrates the successful implementation of the ML-TPM model for automating toxicity prediction with accuracy comparable to conventional physical analyses. Our findings provide valuable insights into the identification of CIM risk factors among gastrointestinal cancer patients undergoing chemotherapy. Furthermore, the results underscore the potential of machine learning in enhancing our understanding of chemotherapy-induced mucositis and advancing personalized patient care strategies.
Real-world treatment patterns and effectiveness of palbociclib plus an aromatase inhibitor in patients with metastatic breast cancer aged 75 years or older
Authors: Brufsky Adam , Liu Xianchen , Li Benjamin , McRoy Lynn , Chen Connie , Layman Rachel M. , Rugo Hope S. 
Published: September 2023

BackgroundElderly patients are generally underrepresented in oncology clinical trials; therefore, real-world data are needed to inform clinical management of elderly patients with hormone receptor–positive/human epidermal growth factor receptor 2–negative (HR+/HER2−) metastatic breast cancer (mBC). This subanalysis of the P-REALITY X study (NCT05361655) evaluated palbociclib treatment patterns and comparative effectiveness of palbociclib plus an aromatase inhibitor (AI) versus an AI alone among patients with HR+/HER2− mBC aged ≥ 75 years treated in routine clinical practice in the United States.MethodsThis retrospective observational cohort study used electronic health records from the Flatiron Health Analytic Database. Palbociclib treatment patterns, overall survival (OS), real-world progression-free survival (rwPFS), and time to chemotherapy (TTC) were evaluated. Three methods were used for comparative analyses: (1) an unadjusted analysis, (2) stabilized inverse probability treatment weighting (sIPTW; primary analysis), and (3) propensity score matching (PSM; sensitivity analysis).ResultsA total of 961 patients aged ≥ 75 years with HR+/HER2− mBC were identified who started palbociclib plus an AI (n = 313) or an AI alone (n = 648) as first-line (1L) therapy between February 2015 and March 2020 (data cut-off: September 30, 2020). Among patients in the palbociclib plus an AI group with a documented palbociclib starting dose (n = 306), approximately 75% started palbociclib at 125 mg/day, and approximately 40% experienced dose adjustment. After sIPTW, patients treated with palbociclib plus an AI versus an AI alone had significantly improved OS (median of 43.0 vs. 32.4 months; hazard ratio [HR], 0.66 [95% confidence interval (CI), 0.51–0.84]; P = 0.0007), rwPFS (median of 20.0 vs. 15.0 months; HR, 0.72 (0.59–0.89); P = 0.0021), and TTC (median of 40.2 vs. 27.4 months; HR, 0.69 [0.55–0.87]; P = 0.0014). These significant improvements in OS, rwPFS, and TTC remained consistent in the unadjusted analysis and after PSM.ConclusionThis real-world comparative analysis demonstrated that 1L palbociclib plus an AI is associated with improved effectiveness compared with an AI alone among patients with HR+/HER2− mBC aged ≥ 75 years. These findings support palbociclib plus an AI as a standard-of-care 1L treatment for elderly patients with HR+/HER2− mBC.
An online home-based exercise program improves autonomic dysfunction in breast cancer survivors
Authors: Lavín-Pérez Ana Myriam , Collado-Mateo Daniel , Hinojo González Carmen , Batista Marco , Mayo Xián , Ruisánchez Villar Cristina , Jiménez Alfonso 
Published: September 2023

Introduction: Exercise interventions for breast cancer survivors have proved their potential to improve clinical, physical, and psychosocial outcomes. However, limited studies have explored exercise effects on autonomic dysfunction and the measurement of exercise tolerance and progression through daily heart rate variability (HRV).Purpose: To analyze the effects of a 16-wk exercise intervention on the autonomic modulation of breast cancer survivors, as well as to examine the evolution of daily measured HRV and its interaction with exercise sessions in this population.Methods: A total of 29 patients who had undergone chemotherapy and radiotherapy were randomly assigned to the exercise group or to the control group. The exercise intervention was delivered remotely through online meetings and consisted of supervised training resistance and cardiovascular exercise 3 times per week. During the intervention all patients measured their HRV daily obtaining the napierian logarithm of the root mean square of successive differences between normal heartbeats (lnrMSSD) and the napierian logarithm of the standard deviation of the interbeat interval of normal sinus beats (lnSDNN) values at four moments: day 0 (the morning of the training sessions), 24, 48, and 72 h after exercise.Results: The results revealed a significant interaction between group and months during the intervention period for lnrMSSD and lnSDNN (p &lt; 0.001). Additionally, there were significant differences in lnSDNN recovery time between months (p &lt; 0.05), while differences in lnrMSSD become apparent only 24 h after exercise (p = 0.019). The control group experienced a significant decrease in both variables monthly (p &lt; 0.05) while exercise group experienced a significant increment (p &lt; 0.05).Conclusion: HRV is daily affected by exercise training sessions in cancer patients. Although results strongly support the role of exercise as a post-chemotherapy and radiotherapy rehabilitation strategy for breast cancer survivors to improve autonomic imbalance, further research is necessary to validate these initial findings.