bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal analysis of systemic ventricular function and atrioventricular valve function after the Fontan procedure
Authors: Dahmen Vincent , Heinisch Paul Philipp , Staehler Helena , Schaeffer Thibault , Burri Melchior , Röhlig Christoph , Klawonn Frank , Hager Alfred , Ewert Peter , Hörer Jürgen , Ono Masamichi 
Published: March 2023

Abstract

OBJECTIVES
This study aimed to determine the longitudinal change of systemic ventricular function and atrioventricular valve (AVV) regurgitation after total cavopulmonary connection (TCPC).

METHODS
In 620 patients who underwent TCPC between 1994 and 2021, 4219 longitudinal echocardiographic examinations of systemic ventricular function and AVV regurgitation were evaluated retrospectively.

RESULTS
The most frequent primary diagnosis was hypoplastic left heart syndrome in 172, followed by single ventricle in 131, tricuspid atresia in 95 and double inlet left ventricle (LV) in 91 patients. Dominant right ventricle (RV) was observed in 329 (53%) and dominant LV in 291 (47%). The median age at TCPC was 2.3 (1.8–3.4) years. Transplant-free survival at 5, 10 and 15 years after TCPC was 96.3%, 94.7% and 93.6%, respectively, in patients with dominant RV and 97.3%, 94.6% and 94.6%, respectively, in those with dominant LV (P = 0.987). Longitudinal analysis of systemic ventricular function was similar in both groups during the first 10 years postoperatively. Thereafter, systemic ventricular function worsened significantly in patients with dominant RV, compared with those with dominant LV (15 years: P = 0.007, 20 years: P = 0.03). AVV regurgitation was more frequent after TCPC in patients with dominant RV compared with those with dominant LV (P &lt; 0.001 at 3 months, 3 years, 5 years, 10 years and 15 years, P = 0.023 at 20 years). There was a significant correlation between postoperative systemic ventricular dysfunction and AVV regurgitation (P &lt; 0.001).

CONCLUSIONS
There were no transplant-free survival difference and no difference in ventricular function between dominant RV and dominant LV for the first 10 years after TCPC. Thereafter, ventricular function in dominant RV was inferior to that in dominant LV. The degree of AVV regurgitation was significantly higher in dominant RV, compared with dominant LV, and it was positively associated with ventricular dysfunction, especially in dominant RV.
Effects of exercise training on cardiac toxicity markers in women with breast cancer undergoing chemotherapy with anthracyclines: a randomized controlled trial
Authors: Antunes Pedro , Joaquim Ana , Sampaio Francisco , Nunes Célia , Ascensão António , Vilela Eduardo , Teixeira Madalena , Capela Andreia , Amarelo Anabela , Marques Cristiana , Viamonte Sofia , Alves Alberto , Esteves Dulce 
Published: March 2023

Abstract

Aims
Exercise training has been suggested to prevent anthracycline-related cardiac dysfunction, but clinicalbased evidence is scarce. We investigated the effects of a supervised exercise training programme (SETP) on cardiac toxicity markers in women with breast cancer (BC) receiving anthracycline-containing chemotherapy.

Methods and results
Ninety-three women with early-stage breast cancer were randomly allocated to a supervised exercise training programme (SETP) plus usual care group (Exercise, n = 47) or usual care alone group (UC, n = 46). The SETP consisted of three sessions per week, combining aerobic and resistance training, conducted concurrently across the anthracycline-containing chemotherapy length. The primary endpoint was the change in left ventricular ejection fraction (LVEF) from baseline to the end of anthracycline cycles. Secondary endpoints included global longitudinal strain (GLS) and other conventional echocardiographic parameters, cardiorespiratory fitness (estimated peak VO2), circulating biomarkers (NT-proBNP, hs-TnT), and safety of the SETP. The study endpoints were also assessed 3 months after the end of anthracycline cycles. All patients were prescribed four cycles of doxorubicin plus cyclophosphamide (AC). No significant between-group differences in LVEF change were seen at the end of AC [mean difference: 0.7%; 95% confidence interval (CI): −0.8, 2.3; P = 0.349] and 3 months after AC (1.1%; 95% CI: −0.5, 2.6; P = 0.196). Compared to the usual care (UC) group, the estimated peak VO2 increased in the Exercise group at the end of AC (1.6 mL O2·kg−1·min−1; 95% CI: 0.06, 3.1; P = 0.041) and 3 months after AC (3.1 mL O2·kg−1·min−1; 95% CI: 1.4, 4.7; P &lt; 0.001). No between-group differences were found in the remaining secondary endpoints. No serious adverse events were observed during SETP.

Conclusion
Exercise training was safe during chemotherapy and significantly improved cardiorespiratory fitness. No significant effects were seen on cardiac toxicity markers (LVEF or GLS) as compared to the usual care.

Trial registration
Mama Move Gaia on treatment trial ISRCTN32617901
Development of the ¡Vamos por Mas! parenting program to prevent substance use among Chilean adolescents
Authors: Meyer Jessica K V , Ortiz de Rozas Amaia Bernard , Muñoz Ivan , Burmeister Catalina , Macchiavello Carlo , Lendzion Lauren , García-Huidobro Diego 
Published: March 2023

SummaryIn Chile, the prevalence of tobacco, alcohol and drug use among adolescents is very high. Decades of research indicate that parenting interventions reduce these risky behaviors. However, there are no parenting interventions validated in Chile to prevent adolescent substance use. This article reports the development of the ¡Vamos por Mas! (¡VxM!) program following the recommendations of the Medical Research Council’s framework for designing and evaluating complex interventions. After identifying key intervention components, a preliminary version of a substance-use prevention program was designed. The preliminary intervention targeted families with adolescents in fifth and sixth grade and had four components: personalized feedback, in-person workshops, virtual engagement, and family support, to deliver positive-youth development and family-strengthening content. Then, students, guardians, school staff and community experts from different school systems (N = 111) evaluated the preliminary version of the program through a convergent parallel mixed methods study, including focus groups (N = 14) and surveys (N = 101). In general, all participants had positive perceptions of the program and valued its purpose, strategies, objectives and contents. Suggestions included expanding the purpose to promote healthy relationships, focusing on schools with low and intermediate socioeconomic vulnerability, including self-control content, removing the personalized feedback component and adding two additional components: school partnership and external supervision, among other improvements. With this information, the final version of the ¡VxM! program was developed. After a rigorous intervention development process, the ¡VxM! program is ready to be piloted and evaluated in a randomized trial.
Identification of clade-wide putative cis-regulatory elements from conserved non-coding sequences in Cucurbitaceae genomes
Authors: Song Hongtao , Wang Qi , Zhang Zhonghua , Lin Kui , Pang Erli 
Published: March 2023

Abstract
Cis-regulatory elements regulate gene expression and play an essential role in the development and physiology of organisms. Many conserved non-coding sequences (CNSs) function as cis-regulatory elements. They control the development of various lineages. However, predicting clade-wide cis-regulatory elements across several closely related species remains challenging. Based on the relationship between CNSs and cis-regulatory elements, we present a computational approach that predicts the clade-wide putative cis-regulatory elements in 12 Cucurbitaceae genomes. Using 12-way whole-genome alignment, we first obtained 632 112 CNSs in Cucurbitaceae. Next, we identified 16 552 Cucurbitaceae-wide cis-regulatory elements based on collinearity among all 12 Cucurbitaceae plants. Furthermore, we predicted 3 271 potential regulatory pairs in the cucumber genome, of which 98 were verified using integrative RNA sequencing and ChIP sequencing datasets from samples collected during various fruit development stages. The CNSs, Cucurbitaceae-wide cis-regulatory elements, and their target genes are accessible at http://cmb.bnu.edu.cn/cisRCNEs_cucurbit/. These elements are valuable resources for functionally annotating CNSs and their regulatory roles in Cucurbitaceae genomes.
Evaluating Dermal Bone as a Novel Source of Endocrine Information in Ninespine and Threespine Stickleback Fish
Authors: Dillon D , Witten P E , Buck C L 
Published: March 2023

SynopsisMonitoring the physiology of small aquatic and marine teleost fish presents challenges. Blood samples, often the first choice for endocrinologists, can be difficult or even impossible to obtain and alternative matrices currently used for hormone analyses do not occur in fishes (e.g., hair, feathers etc.) or are not easily collected from small aquatic organisms (e.g., urine and feces). Some teleosts, however, have enlarged bony dermal elements that possibly accumulate and store steroid hormones in physiological relevant concentrations. Both threespine stickleback (Gasterosteus aculeatus) and ninespine stickleback (Pungitius pungitius) have a series of external, lateral bony plates, dorsal spines, and a pair of pelvic spines attached to the pelvic girdle. We investigated if cortisol, the primary circulating glucocorticoid in teleosts, could be extracted from stickleback dermal bone and quantified using a commercially available enzyme immunoassay (EIA). We successfully validated a cortisol EIA for dermal bone extracts, determined that cortisol was detectable in both species, and found that dermal bone cortisol levels significantly correlated with cortisol levels in whole body homogenate. Ninespine stickleback had significantly higher dermal bone cortisol concentrations than threespine stickleback and female threespine stickleback tended to have over twice the mean dermal bone cortisol concentration than males. Because both stickleback species are widely used for ecotoxicological studies, using dermal bone as a source of endocrine information, while leaving the body for contaminant, genomic, histological, and stable isotope analyses, could be a powerful and parsimonious tool. Further investigation and physiological validations are necessary to fully understand the utility of this new sample matrix.
Designing and Evaluating Aventura Marina: A Serious Game to Promote Visitors’ Engagement in a Science Museum Exhibition
Authors: Cordova-Rangel Jessica , Caro Karina 
Published: March 2023

AbstractMuseums have employed video games in their exhibitions to foster visitors’ participation and improve their learning process through a positive experience. In this work, we present Aventura Marina, a serious video game inspired by escape room game mechanics, composed of an app and an authoring tool, which aims to promote visitors’ engagement with the new exhibition Sea Hall of the Caracol Museo de Ciencias, a Mexican museum in the northwest of Mexico. To design Aventura Marina, we followed a user-centered design approach, based on the design thinking methodology. We evaluated the high-fidelity prototype of the game and the low-fidelity prototype of the authoring tool with museum staff and potential visitors. Our results indicate that Aventura Marina has the potential to promote visitors’ engagement with the Sea Hall exhibition as participants indicated having a positive experience, recognized that they were more interested in and less bored by the topics of the exhibition and that they were eager to play the game again. In relation to the authoring tool, museum staff mentioned how this project could be implemented in other halls of the museum. Finally, we discuss the improvements for a future iteration of the prototypes and how this kind of technology can complement the content of museum exhibitions.
Linking rare and common disease vocabularies by mapping between the human phenotype ontology and phecodes
Authors: McArthur Evonne , Bastarache Lisa , Capra John A 
Published: March 2023

AbstractEnabling discovery across the spectrum of rare and common diseases requires the integration of biological knowledge with clinical data; however, differences in terminologies present a major barrier. For example, the Human Phenotype Ontology (HPO) is the primary vocabulary for describing features of rare diseases, while most clinical encounters use International Classification of Diseases (ICD) billing codes. ICD codes are further organized into clinically meaningful phenotypes via phecodes. Despite their prevalence, no robust phenome-wide disease mapping between HPO and phecodes/ICD exists. Here, we synthesize evidence using diverse sources and methods—including text matching, the National Library of Medicine’s Unified Medical Language System (UMLS), Wikipedia, SORTA, and PheMap—to define a mapping between phecodes and HPO terms via 38 950 links. We evaluate the precision and recall for each domain of evidence, both individually and jointly. This flexibility permits users to tailor the HPO–phecode links for diverse applications along the spectrum of monogenic to polygenic diseases.
Comprehensive Evaluation of an HPLC–MS-MS Method for Quantitation of Seven Anti-Coagulant Rodenticides and Dicoumarol in Animal Serum
Authors: Francis Kyle A , Tkachenko Andriy , Johnson Joseph T , Smith Lori L , Noonan Robyn T , Filigenzi Michael S , Gaskill Cynthia L , Romano Megan C 
Published: March 2023

AbstractAnti-coagulant rodenticides (ARs) are commonly utilized for controlling rodent populations; however, non-target companion and wildlife animals are also exposed. A method was developed for quantitation of seven ARs (chlorophacinone, coumachlor, bromadiolone, brodifacoum, difethialone, diphacinone and warfarin) and dicoumarol (a naturally occurring anti-coagulant) in animal serum. Analytes were extracted with 10% (v/v) acetone in methanol and analyzed by reverse phase high-performance liquid chromatography–tandem mass spectrometry using electrospray ionization (negative mode) combined with multiple reaction monitoring. In-house method validation in the originating laboratory using non-blinded samples revealed method limits of quantitation at 2.5 ng/mL for all analytes. The inter-assay accuracy ranged from 99% to 104%, and the relative standard deviation ranged from 3.5% to 20.5%. Method performance was then verified in the originating laboratory during an exercise organized by an independent party using blinded samples. The method was successfully transferred to two naïve laboratories and further evaluated for reproducibility among three laboratories by means of Horwitz ratio (HorRat(R)) values. Such extensive validation provides a high degree of confidence that the method is rugged, robust, and will perform as expected if used by others in the future.
Technological and Organizational Change and the Careers of Workers
Authors: Battisti Michele , Dustmann Christian , Schönberg Uta 
Published: March 2023

Abstract
This paper investigates the effects of technological and organizational change (T&O) on jobs and workers. We show that although T&O reduces firm demand for routine relative to abstract task-based jobs, affected workers do not face higher probability of non-employment or lower earnings growth than unaffected workers. Rather, firms that adopt T&O offer routine workers retraining opportunities to upgrade to more abstract jobs. Older workers form an important exception: T&O increases the risk that they permanently withdraw from the labor market and reduces their earnings, regardless of the tasks they performed in the firm prior to T&O.
A study of ionospheric impacts in the wireless electromagnetic exploration with the QWE method
Authors: Zhang Yilang , Gao Ya , Fu Changmin 
Published: March 2023

AbstractThe wireless electromagnetic method (WEM) is an emerging new method for deep resource exploration. This method, based on magnetotellurics and the controlled-source electromagnetic method, features advantages such as high signal strength and signal-to-noise ratio, large penetration depth, and easy survey geometry setups, which gives WEM potential for deployment in mineral exploration of large depths. As for now, there is little research into the controlling factors of the ionosphere in WEM exploration, and whether the ionosphere is related to the modeling result remains unclear. In this work, we first developed a modified quadrature with extrapolation (QWE) method to settle the oscillation in the calculation of a WEM response. Then we compared its performance and accuracy with other methods and find that our method has better numerical stability even with highly oscillated integral kernels, which is ideal for WEM emulation. With the emulation tools, we can further investigate how the ionosphere’s height and resistivity affect the ground-received signal. We compared signals obtained with different ionospheric properties under various earth resistivity models. The conclusion is that these ionospheric properties only impact the received signal’s strength and have no influence on the Cagniard resistivity or phase of impedance.
Bacterial DNA promoting inflammation via the Sgk1/Nedd4L/Syk pathway in mast cells contributes to antihistamine-nonresponsive CSU
Authors: Chen Bangtao , Song Yao , Yang Xiongbo , Yang Jing , Hao Fei 
Published: March 2023

Abstract
Inflammation centered on non-IgE-mediated mast cell activation characterizes chronic spontaneous urticaria resistant to nonsedating H1-antihistamines. We recently uncovered a strong positive association between inflammation and the fecal Escherichia. To further explore the actions of bacterial DNA derived from Escherichia on mast cells, intestinal permeability of patients with chronic spontaneous urticaria with or without nonsedating H1-antihistamine resistance and healthy controls were determined, and LAD2 cells with knockdown of Syk, Nedd4L, or Sgk1 or with incubation of inhibitors GS9973, GSK650394, and MG132 were posttreated with btDNA. We found that (i) serum intestinal permeability indices and bacterial DNA markedly increased in patients with chronic spontaneous urticaria with nonsedating H1-antihistamine resistance compared with those without (all P &lt; 0.001), and bacterial DNA positively correlated with the degree of inflammation; (ii) IL-6 and TNF-α levels were time- and dose-dependently upregulated in bacterial DNA–stimulated LAD2 cells, which relied on unmethylated CpG in bacterial DNA and Toll-like receptor 9 protein in cells; (iii) Syk knockdown or inhibition of Syk Tyr525/526 phosphorylation blocked bacterial DNA–initiated cytokine production; (iv) Nedd4L interacted with Tyr525/526-phosphorylated Syk, and inhibition of Nedd4L Ser448 phosphorylation induced by bacterial DNA–activated Sgk1 was mandatory for bacterial DNA's proinflammatory property; and (v) Sgk1 suppression showed an inhibitory effect on bacterial DNA–induced inflammation by ensuring Nedd4L-mediated ubiquitination of Tyr525/526-phosphorylated Syk. Collectively, we identified previously unknown contributory roles of bacterial translocation and serum bacterial DNA on the inflammation phenotype in patients with chronic spontaneous urticaria with nonsedating H1-antihistamine resistance and further uncovered a vital negative regulatory role for the Sgk1/Nedd4L/Syk pathway in bacterial DNA–induced inflammation in LAD2 cells.
Association of metformin, aspirin, and cancer incidence with mortality risk in adults with diabetes
Authors: Orchard Suzanne G , Lockery Jessica E , Broder Jonathan C , Ernst Michael E , Espinoza Sara , Gibbs Peter , Wolfe Rory , Polekhina Galina , Zoungas Sophia , Loomans-Kropp Holli A , Woods Robyn L ,   ASPREE Investigator Group, McNeil John , Woods Robyn , Wolfe Rory , Murray Anne , Chan Andrew , Orchard Suzanne , Lockery Jessica , Nelson Mark , Reid Christorpher , Shah Raj , Newmann Anne , Storey Elsdon , Stocks Nigel , Tonkin Andrew , Espinoza Sara 
Published: March 2023

AbstractBackgroundMetformin and aspirin are commonly co-prescribed to people with diabetes. Metformin may prevent cancer, but in older people (over 70 years), aspirin has been found to increase cancer mortality. This study examined whether metformin reduces cancer mortality and incidence in older people with diabetes; it used randomization to 100 mg aspirin or placebo in the ASPirin in Reducing Events in the Elderly (ASPREE) trial to quantify aspirin’s impact on metformin users.MethodsAnalysis included community-dwelling ASPREE participants (aged ≥70 years, or ≥65 years for members of US minority populations) with diabetes. Diabetes was defined as a fasting blood glucose level greater than 125 mg/dL, self-report of diabetes, or antidiabetic medication use. Cox proportional hazards regression models were used to analyze the association of metformin and a metformin-aspirin interaction with cancer incidence and mortality, with adjustment for confounders.ResultsOf 2045 participants with diabetes at enrollment, 965 were concurrently using metformin. Metformin was associated with a reduced cancer incidence risk (adjusted hazard ratio [HR] = 0.68, 95% confidence interval [CI] = 0.51 to 0.90), but no conclusive benefit for cancer mortality (adjusted HR = 0.72, 95% CI = 0.43 to 1.19). Metformin users randomized to aspirin had greater risk of cancer mortality compared with placebo (HR = 2.53, 95% CI = 1.18 to 5.43), but no effect was seen for cancer incidence (HR = 1.11, 95% CI = 0.75 to 1.64). The possible effect modification of aspirin on cancer mortality, however, was not statistically significant (interaction P = .11).ConclusionsIn community-dwelling older adults with diabetes, metformin use was associated with reduced cancer incidence. Increased cancer mortality risk in metformin users randomized to aspirin warrants further investigation.ASPREE Trial RegistrationClinicalTrials.gov ID NCT01038583
Functional and phylogenetic similarities of co-occurring invaders affect the growth of an invasive forb
Authors: Ren Jie , Chen Pengdong , Shen Changchao , Tao Zhibin , Huang Wei 
Published: March 2023

AbstractExpansion of global trade and acceleration of climate change dramatically promote plant invasions. As a result, a large number of habitats harbor multiple invasive plant species. However, patterns of invasive interactions and the drivers mediating their interactions remain unclear. In this greenhouse, potted plant study, we tested the impacts of 18 invasive plant species on the growth of target invader Erigeron canadensis which is dominant in central China. Neighboring invasive species belong to three functional groups (grass, forb and legume) and have different levels of relatedness to E. canadensis. Growth of E. canadensis’ strongly depended on the identity of neighboring invaders. Some neighboring invasive species suppressed growth of E. canadensis, others had no effect, while some promoted growth of E. canadensis. Through analyses of functional and phylogenetic similarities between the target species and neighboring invaders, we showed that two factors probably play roles in determining the relative responses of E. canadensis. Generally, E. canadensis responded negatively to invasive grasses and forbs, while it responded positively to invasive legumes. Furthermore, the negative responses to neighboring invasive grasses and forbs increased with increasing phylogenetic distance between the neighbors and E. canadensis. In contrast, the positive responses to invasive legumes did not depend on phylogenetic distance from E. canadensis. Our results suggest that successful invasion of E. canadensis probably depends on the identity of co-occurring invasive plant species. Interactions between E. canadensis and other invasive species should help managers select management priorities.