bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Situ Electrical Detection of Methane Oxidation on Atomically Thin IrO2 Nanosheet Films Down to Room Temperature
Authors: Ishihara Yoshiaki , Koitaya Takanori , Hamahiga Yuto , Sugimoto Wataru , Yamamoto Susumu , Matsuda Iwao , Yoshinobu Jun , Nouchi Ryo 
Published: June 2023

AbstractActivation of the CH bond is the first step in converting methane into valuable chemicals. Herein, the successful induction and electrical detection of a methane oxidation reaction are reported at room temperature using IrO2 nanosheets, a 2D form of IrO2. A clear decrease in electrical resistance upon exposure to methane is observed by using atomically thin IrO2 nanosheet films. The resistance decrease disappears upon simultaneous exposure to oxygen, suggesting that methane is oxidized by consuming the lattice oxygen of the IrO2 nanosheets and that the oxygen vacancies generated are recovered by oxygen in the atmosphere. The resistance decrease is observed even at 300 K, indicating the high methane oxidation ability of the IrO2 nanosheets. These results are confirmed by a shift of the Ir 4f peaks in ambient pressure X‐ray photoelectron spectra. Furthermore, deposition of amorphous carbon, that is, methane oxidation products, on IrO2 nanosheets is also confirmed by Raman scattering spectroscopy after prolonged methane exposure at high temperatures in the absence of oxygen. This study demonstrates the ability of IrO2 nanosheets to oxidize methane at least down to 300 K and is an important example of the usefulness and simplicity of chemical reaction monitoring using electrical resistance changes.
From Pyridine to (−)‐Agelastatin A
Authors: Vale João R. , Fortunato Milene A. G. , Andrade Késsia H. S. , Rocha Ângelo M. R. , Afonso Carlos A. M. , Siopa Filipa 
Published: June 2023

Abstract(−)‐Agelastatin A was synthetized employing a flow photorearrangement of a pyridinium salt, constructing in one step the cyclopentene core possessing the desired functionalities and relative configurations. A flow enzymatic kinetic resolution of the resulting bicyclic vinyl aziridine delivered the enantiopure precursor to the natural product. This total synthesis required the use of a single protective group. Two novel agelastatin N3‐derivatives were synthesized and their cytotoxicity evaluated against a series of cancer cell lines, which corroborated the importance of unsubstituted N3 in the biological activity of (−)‐agelastatin A.
Role of the Gut Microbiota and Its Metabolites in Tumorigenesis or Development of Colorectal Cancer
Authors: Qu Ruize , Zhang Yi , Ma Yanpeng , Zhou Xin , Sun Lulu , Jiang Changtao , Zhang Zhipeng , Fu Wei 
Published: June 2023

AbstractColorectal cancer (CRC) is the most common cancer of the digestive system with high mortality and morbidity rates. Gut microbiota is found in the intestines, especially the colorectum, and has structured crosstalk interactions with the host that affect several physiological processes. The gut microbiota include CRC‐promoting bacterial species, such as Fusobacterium nucleatum, Escherichia coli, and Bacteroides fragilis, and CRC‐protecting bacterial species, such as Clostridium butyricum, Streptococcus thermophilus, and Lacticaseibacillus paracasei, which along with other microorganisms, such as viruses and fungi, play critical roles in the development of CRC. Different bacterial features are identified in patients with early‐onset CRC, combined with different patterns between fecal and intratumoral microbiota. The gut microbiota may be beneficial in the diagnosis and treatment of CRC; some bacteria may serve as biomarkers while others as regulators of chemotherapy and immunotherapy. Furthermore, metabolites produced by the gut microbiota play essential roles in the crosstalk with CRC cells. Harmful metabolites include some primary bile acids and short‐chain fatty acids, whereas others, including ursodeoxycholic acid and butyrate, are beneficial and impede tumor development and progression. This review focuses on the gut microbiota and its metabolites, and their potential roles in the development, diagnosis, and treatment of CRC.
Study on the antibacterial effect of CuO nanoparticles on Klebsiella pneumonia bacteria: Efficient treatment for colorectal cancer
Authors: Valadbeigi Maria , Mahmoudifard Matin , Ganji Shahla Mohammad , Mehrabian Sedigheh 
Published: June 2023

AbstractColorectal cancer (CRC) is a widespread type of cancer across the world. One efficient therapy approach is the use of antibiotic agents, but one of the main issues related to treating CRC is microbial resistance to antibiotics. As microbes are becoming more resistant to antibiotics and other traditional antimicrobial agents, nanobiotechnology has made it possible to employ nanomaterials with the aim of creating a new generation of antimicrobial agents. In the present study, we have assessed the antimicrobial potential of CuO nanoparticles (NPs) against gram‐negative bacteria like Klebsiella pneumoniae carrying PKS genes responsible for encoding colibactin as the key factor for CRC development. For this purpose, the antibacterial effects of conventional antibacterial agents, including erythromycin, piperacillin, and ampicillin, as well as CuONPs, were compared on isolated strains from cancerous candidates. The obtained results revealed that isolates (K. pneumoniae) showed resistance toward the mentioned conventional antibiotics, but CuONPs showed efficient antibacterial properties against K. pneumonia with a MIC = 62 μg/mL. On the other hand, a synergistic antibacterial effect was obtained when CuONPs were used in combination with conventional antibiotics, which are ineffective when used alone.Therefore, CuONPs can be introduced as an excellent antimicrobial agent against K. pneumoniae bacteria in CRC, especially when they are combined with other antibiotics since they can activate the antimicrobial activity of the conventional antibiotics.
What is happening to immunohistochemistry?
Authors: Gautron Laurent 
Published: June 2023

AbstractImmunohistochemistry is a commonly used technique in research and pathology laboratories worldwide. However, in recent years, there has been a significant decrease in the number of Pubmed entries using the term immunohistochemistry. This decline can be attributed to two factors: increased awareness of the issue of unreliable research antibodies and the availability of novel RNA in situ hybridization techniques. Using the example of immunohistochemistry, this text discusses the factors that can affect good laboratory and publishing practices, or their lack thereof.
IPTG‐induced high protein expression for whole‐cell biosynthesis of L‐phosphinothricin
Authors: Xu Jian‐Miao , Wu Zhou‐Sheng , Zhao Ke‐Ji , Xi Zhi‐Jie , Wang Liu‐Yu , Cheng Feng , Xue Ya‐Ping , Zheng Yu‐Guo 
Published: June 2023

AbstractBackgroundBiocatalytic production of L‐phosphinothricin (L‐PPT) is currently the most promising method. In this work, we use an Escherichia coli strain coexpressing of D‐amino acid oxidase and catalase (E. coli DAAO‐CAT) to oxidation biocatalytic D‐PPT to PPO, then use the second E. coli strain coexpressing glutamate dehydrogenase and formate dehydrogenase (E. coli GluDH‐FDH) to reduce biocatalytic PPO to L‐PPT.Main Methods and Major ResultsWe compared the effects of different concentrations of IPTG or lactose on protein expression and enzyme activity in 5 L fermenter. The best induction conditions for E. coli DAAO‐CAT were 0.05 mM IPTG, induction for 18 h at 28°C. The specific enzyme activities of DAAO and CAT were 153.20 U g−1 and 896.23 U g−1, respectively. The optimal induction conditions for E. coli GluDH‐FDH were 0.2 mM IPTG, induction for 19 h at 28°C. The specific enzyme activities of GluDH and FDH were 41.72 U g−1 and 109.70 U g−1, respectively. The 200 mM D‐PPT was biocatalyzed by E. coli DAAO‐CAT for 4 h with space‐time yield of 9.0 g·L−1·h−1 and conversion rate of over 99.0%. Then 220 mM PPO was converted to L‐PPT by E. coli GluDH‐FDH for 3 h with space‐time yield of 14.5 g·L−1·h−1 and conversion rate of over 99.0%. To our knowledge, this is the most efficient biocatalytic reaction for L‐PPT production.Conclusions and ImplicationsWe found that IPTG has advantages compared with lactose in the enzyme activity and biomass of E. coli DAAO‐CAT and E. coli GluDH‐FDH, and IPTG is more environmentally friendly. Our data implicated that IPTG can replace lactose in terms of economic feasibility and effectiveness for scaled‐up industrial fermentations.
On learning platform metrics as markers for student success in a course
Authors: Yalcin Ali , Kaw Autar , Clark Renee 
Published: June 2023

AbstractAdaptive learning platforms are increasingly being used as part of varying instructional modalities. Particularly relevant to this paper, adaptive learning is a critical component of personalized, preclass learning in a flipped classroom. Previously inaccessible, data generated by adaptive learning platforms regarding student engagement with the course content provides an invaluable opportunity to gain a deeper understanding of the learning process and improve upon it. We aim to investigate the relationships between adaptive learning platform interactions and overall student success in the course and identify the variables most influential to student success. We present a comprehensive analysis of our adaptive learning platform data collected in a Numerical Methods course, including aggregate statistics, frequency analysis, and Principal Component Analysis, to determine which variables exhibited the most variability and, therefore, the most information in the data. Subsequently, we used the Partitioning Around Medoids clustering approach to investigate naturally occurring clusters of students and how these clusters relate to overall performance in the course. Our results show that overall performance in the course, as measured by the final course grade, is strongly associated with (1) the behavioral interactions of students with the adaptive platform and (2) their performance on the adaptive learning assessments. We also found distinct student clusters (as defined by success in the course) that exhibited distinctly different behaviors. These findings provide qualitative and quantitative information to identify students needing support and to craft an evidence‐based support strategy for these students.
The interplay of cardiac hemodynamics in rheumatic triple valve stenosis
Authors: Velayutham Ramanathan , Ahmed A Shaheer 
Published: June 2023

AbstractRheumatic heart disease is among the common causes of cardiovascular morbidity in developing countries. Here we present the hemodynamic interplay of stenotic rhematic involvement of aortic, mitral, and tricuspid valves in a 35‐year‐old female. Though noninvasive imaging by echocardiography and doppler has taken the upper hand today, this case illustrates the crucial role of cardiac catheterization in understanding the hemodynamics and patient management of rheumatic multivalvular heart disease.
Improved Luminous Efficiency of AgInS2 Quantum Dots and Zeolitic Imidazolate Framework‐70 Composite for White Light Emitting Diode Applications
Authors: Qin Zhe , Chen Yuanhong , Chen Ting , Liang Mengbiao , Wen Peng , Guo Chunxian , Ye Changqing , Xie Zhixiang 
Published: June 2023

AbstractThe application of multiple quantum dots (QDs) in the field of white light emitting diodes (WLEDs) is still an important challenge due to their low luminous efficiency and quenching phenomenon. In this paper, we prepared AgInS2 QDs/zeolitic imidazolate framework‐70 (AIS/ZIF‐70) composite by a microwave hydrothermal method. Owing to the high porosity and stability of ZIF‐70, it could effectively prevent quenching issues due to the aggregation of QDs. Since the ZIF‐70 and QDs were chemically bonded, the formation of the ZnS layer could effectively passivate the surface defect and thus the quantum yield reached 21.49 % in aqueous solution. The luminous efficiency (LE) of the assembled AIS/ZIF‐based WLED was reinforced by 6.8 times with a molar ratio of AgIn/Zn=18, i. e. at 5.26 % molar fraction of ZIF‐70. Moreover, the color rendering index (CRI) and correlated color temperature (CCT) of AIS/ZIF‐based WLED were 84.3 and 3631 K, respectively, indicating its potential application in solid‐state lighting.
Extended π‐Conjugation and Structural Planarity Effects of Symmetrical D‐π‐A‐π‐D Naphthalene and Perylene Diimide Semiconductors on n‐type Electrical Properties
Authors: Gámez‐Valenzuela Sergio , Torres‐Moya Iván , Sánchez Abelardo , Donoso Beatriz , López Navarrete Juan Teodomiro , Ruiz Delgado M. Carmen , Prieto Pilar , Ponce Ortiz Rocío 
Published: June 2023

AbstractA series of donor‐π‐acceptor‐π‐donor (D‐π‐A‐π‐D) compounds based on naphthalendiimide (NDI) and perylenediimide (PDI) central cores combined with triphenylamine and phenylcarbazole donor groups have been synthesized, characterized and tested in top‐contact/bottom gate organic field‐effect transistors (OFETs). The results showed high electron mobilities, up to 0.3 cm2 V−1 s−1, in the case of NDI derivatives and moderate values of around 10−3 cm2 V−1 s−1 for PDI‐based semiconductors. Quantum chemical calculations were performed in order to support the experimental data. The results suggest that adequate molecular characteristics and larger crystalline domains in NDI vs. PDI semiconducting films may be the reasons behind the enhanced electrical properties of NDI derivatives. Furthermore, when the lateral donor substituents are triphenylamine groups, the mobilities were slightly higher in comparison to phenylcarbazole donor groups due to an improved electron‐donating character. Other characterization techniques, such as AFM, X‐ray diffraction or spectroelectrochemistry, among others, have been performed to analyze supramolecular order, charge carriers’ nature and stability, parameters closely related to charge transport characteristics.
The emerging role of septins in fungal pathogenesis
Authors: Eisermann Iris , Garduño‐Rosales Marisela , Talbot Nicholas J. 
Published: June 2023

AbstractFungal pathogens undergo specific morphogenetic transitions in order to breach the outer surfaces of plants and invade the underlying host tissue. The ability to change cell shape and switch between non‐polarised and polarised growth habits is therefore critical to the lifestyle of plant pathogens. Infection‐related development involves remodelling of the cytoskeleton, plasma membrane and cell wall at specific points during fungal pathogenesis. Septin GTPases are components of the cytoskeleton that play pivotal roles in actin remodelling, micron‐scale plasma membrane curvature sensing and cell polarity. Septin assemblages, such as rings, collars and gauzes, are known to have important roles in cell shape changes and are implicated in formation of specialised infection structures to enter plant cells. Here, we review and compare the reported functions of septins of plant pathogenic fungi, with a special focus on invasive growth. Finally, we discuss septins as potential targets for broad‐spectrum antifungal plant protection strategies.
Facile Synthesis and Insight of Atomically Dispersed Ni Catalyst on N‐Doped Carbonized Lignin for Highly Efficient Electrochemical CO2 Reduction to CO
Authors: Park Gi‐Dong , Sirisomboonchai Suchada , Norinaga Koyo 
Published: June 2023

AbstractFor the electrochemical CO2 reduction reaction (CO2RR), the single‐metal atom catalysts (SACs) on N‐doped carbon are considered promising alternatives to conventional catalysts owing to their unique electrocatalytic properties. However, environmentally friendly methods to prepare SACs are still required. Herein, Ni SAC was synthesized using lignin derived from biomass whose structural and chemical properties render it suitable as both a base carbon matrix and a metal chelating agent. The coordination environment of active Ni−Nx sites was readily manipulated by controlling thermal activation. The Ni SAC on lignin‐derived N‐doped carbon achieved an outstanding CO Faradaic efficiency of 98.2 % at −0.9 V vs. RHE, which is comparable to those of conventional SACs. Experimental results combined with DFT calculations demonstrate the optimal conditions for manufacturing Ni SAC which is highly selective for CO2‐to‐CO conversion and the effect of the electronic structure of Ni atom on CO2RR kinetics.
The need for epidemiological research on eating disorders in Africa and the Caribbean
Authors: Philip Briyanna , Poku Ohemaa , Attoh‐Okine Naa‐Djama , Presskreischer Rachel 
Published: June 2023

AbstractThe eating disorders field acknowledges the need to include diverse populations in research. Although global diversity has increased in epidemiologic research, there is still a significant lack of research in Africa and the Caribbean. The objectives of this article are to highlight knowledge gaps in these regions and make recommendations to improve future research. We searched the literature about the epidemiology of eating disorders in Africa and the Caribbean and provided a brief summary of research findings and measures used to identify cases. There is a large knowledge gap about the epidemiology of eating disorders across African and Caribbean nations. Knowledge about the cultural appropriateness of measurement instruments and screening tools is also lacking. Inadequate information about the epidemiology of eating disorders in Africa and the Caribbean inhibits the eating disorders research field from identifying who is affected. Further, measures and screening instruments with uncertain cultural validity inhibit the ability to understand case presentations and treatment targets. We recommend that the field advocate for the inclusion of eating disorders in larger population health research and that future research should evaluate the appropriateness of measurement instruments to capture eating disorder cases in African and Caribbean countries.Public significanceThere is a lack of eating disorder research in African and Caribbean countries. The limited studies that do exist show evidence of eating disorders in African and Caribbean countries. Research is needed to develop culturally relevant measures for screening and case identification, and to calculate incidence and prevalence.
Positive tree diversity effects on arboreal spider abundance are tied to canopy cover in a forest experiment
Authors: Butz Elizabeth M. , Schmitt Lauren M. , Parker John D. , Burghardt Karin T. 
Published: June 2023

AbstractHuman actions are decreasing the diversity and complexity of forests, and a mechanistic understanding of how these changes affect predators is needed to maintain ecosystem services, including pest regulation. Using a large‐scale tree diversity experiment, we investigate how spiders respond to trees growing in plots of single or mixed species combinations (4 or 12) by repeatedly sampling 540 trees spanning 15 species. In 2019 (6 years post‐establishment), spider responses to tree diversity varied by tree species. By 2021, diversity had a more consistently positive effect, with trees in 4‐ or 12‐species plots supporting 23% or 50% more spiders, respectively, compared to conspecifics in monocultures. Spiders showed stronger tree species preferences in late summer, and the positive impact of plot diversity doubled. In early summer, the positive diversity effect was tied to higher canopy cover in diverse plots, leading to higher spider densities. This indirect path strengthened in late summer, with an additional direct effect of plot diversity on spiders. Prey availability was higher in diverse plots but was not tied to spider density. Overall, diverse plots supported more predators, partly by increasing available habitat. Adopting planting strategies focused on species mixtures may better maintain higher trophic levels and ecosystem functions.
Hall and rotation effects on magnetohydrodynamics two fluids slip flow of ionized gases via parallel conduit
Authors: Raju T. Linga , Satish P. 
Published: June 2023

AbstractIn a rotational model, the Hall effect on two‐liquid magnetohydrodynamics plasma flows of ionized gases between two nonconducting horizontal channel plates in a slip regime is researched. The produced magnetic field is omitted under the presumption that the Reynolds magnetic number is low. The modeled governing equations are solved by using the existing analytical method so as to obtain solutions in closed form. We use the first order slip to obtain exact results for the velocity, temperature fields, and hence the rates of heat transfer coefficient. On the basis of different values for the Hartmann number, slip, Hall, and rotation parameters, also viscosity, density, height, and electrical conductivity ratios of the two fluids, a parametric investigation of the velocity, temperature fields is initiated. It was concluded that the velocity declined with the augmented values of Hartmann number and the fluid velocity and temperature profiles in both the zones oscillate with the effect of the Hall parameter. In addition to that, the stimulations are present in the heat transfer coefficient rate with various values of the slip and rotational parameters.
Higher rate of progesterone receptor positivity in skeletal metastases of breast cancer with a pathological fracture vs those without fracture
Authors: Rajkovic Stanislav M. , Charalambous Michalis P. , Charalambous Charalambos P. , Simic Ljubica D. , Djuricic Goran J. , Dundjerovic Dusko M. , Miceta Lazar D. , Milicic Biljana R. , Sopta Jelena P. 
Published: June 2023

AbstractIdentifying risk factors for fracture occurrence in breast cancer (BC) skeletal metastases (SM) may guide the management of such bone deposits. There is sparse evidence regarding receptor status in SM and their relationship to fracture occurrence. Our study aimed to determine the relationship between estrogen (ER), progesterone (PR) and HER2 receptor status and Ki‐67 index and fracture occurrence in SM of BC. Exactly 152 samples of SM of BC obtained from individual patients were evaluated. The status of the aforementioned receptors and Ki67 index were determined in SMs samples. Their expression was compared between SM that did and did not develop a fracture. Ninety‐one cases sustained a pathological fracture at the SM site, and 61 did not. Patients who sustained a pathological fracture had a higher rate of PR positivity at their SMs as compared to those with no fracture. There was no significant difference between the two groups concerning ER, HER2+ or Ki67 status. SMs secondary to BC with a fracture are more likely to be PR positive than those with no fracture. Determining the receptor status in SMs may identify high‐risk groups for fracture occurrence, and determining the PR status may also guide surgical and hormonal therapy.
Acid‐catalyzed recyclization of spiroindolinepyrano[3,2‐b]pyrans to 3,4‐dihydroquinolinone derivatives containing allomaltol fragment
Authors: Milyutin Constantine V. , Komogortsev Andrey N. , Lichitsky Boris V. , Melekhina Valeriya G. 
Published: June 2023

AbstractFor the first time, we developed a novel one‐step method for the synthesis of substituted 3,4‐dihydroquinolinones with allomaltol moiety. The suggested approach includes acid‐catalyzed recyclization of spiroindolinepyrano[3,2‐b]pyrans which can be obtained by multicomponent reaction of 3‐hydroxy‐4‐pyranones, isatins and malononitrile. The described transformation is a distinctive feature of 3‐hydroxy‐4‐pyranone derivatives and is not typical for other heterocyclic enols. The advantages of the considered method are the employment of easily accessible starting materials and simple work‐up procedure which can avoid chromatographic purification. The structure of one of synthesized products was determined by x‐ray diffraction.