bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Carabapenem-resistant Enterobacteriaceae in Fresh vegetables
Authors: Ojo A.E. , Ajibola A.T. , Adebajo S.O. , Oloyede A.R. , Ojo O.A. , Chibundu T.Z. 
Published: February 2023

Carbapenem-resistant Enterobacteriacea (CRE) has become a growing food safety issue and an ongoing public-health problem of global dimensions. This research study determined the presence of CRE in fresh vegetables. Vegetables were purchased from the retailers and analyzed for Enterobacteriaceae in line with Standard guidelines. The antibiotic profile of the isolated Enterobacteriaceae was determined using Antibiotic Susceptibility Test (AST). The Modified Hodges test (MHT), Carbapenem inactivation method (CIM), Modified carbapenem inactivation (mCIM), and Ethylenediamine tetraacetic acid (EDTA) carbapenem inactivation (eCIM) were used screening for carbapenem resistance among Multidrug-resistant (MDR) Enterobacteriaceae. Forty-six Enterobacteriaceae were isolated in all. Among organisms isolated were Citrobacter freundi, Enterobacter cloacae, Salmonella typhi, Klebsiella sp, and Escherichia coli. AST of the isolates showed that two out of three third-generation antibiotics used had the highest resistance of 100%. Thirty-six (78.26%) were multidrug-resistant, eighteen were CRE using MHT but using CIM, and sixteen were CRE. Eighteen were CRE using the mCIM method, while 17 were using eCIM.
Conclusion: This study shows that vegetables can be a source of carbapenem-resistant Enterobacteriaceae, and mCIM combined with eCIM is more sensitive in detecting CRE.
Significance and Impact of Study: Ready-to-eat fresh Vegetables contain CRE.
Genetic Characterization of Drought-Tolerant Maize Genotypes Based on SSR Markers
Authors: Abegunde O.I. , Idehen E.O. , Oduwaye O.A. , Fabunmi T.O. , Kehinde O.B. 
Published: February 2023

Using 20 pairs of SSR primers situated on each chromosome of the maize genetic makeup, 27 drought-tolerant maize genotypes obtained from the Germplasm Unit of the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, were examined for diversity. The samples' genomic DNA (gDNA) was extracted using the CTAB procedure specified by CIMMYT (2005). All 20 pairs of SSR markers used in this investigation were extremely polymorphic, resulting in 122 repeatable and polymorphic bands that were scored with an average of 12.20 alleles. Each primer yielded a main allele frequency of no more than 0.5. The average major allele frequency for the 10 primers was 0.3036. With an average of 0.8334, all markers had gene diversity values greater than 0.6. Similar to this, the Polymorphic Information Content (PIC) values had an average of 0.8164 and varied from 0.9051 (primer umc1042) to 0.6639 (Umc1669). Since primer umc1042 best identified this variability, the frequency of the main alleles generated by each primer points to the presence of significant molecular genetic variation across the samples under study. This occurrence is further supported by the gene diversity (GD) and polymorphism information content (PIC) values of the primers. Therefore, it can be inferred that the primers are all appropriate for genotyping genetic diversity. Due to their different genetic relationships, varieties IWD C2 SYN F2 and SAMMAZ 52 have the most diversified genotypes and can be utilised as parent materials in a maize breeding programme.
In vivo assessment of antibacterial and growth promoting effect of soursop (Annona muricata Linn.) leaf extract in broiler chickens
Authors: Kuka T.T. , Agedeson T.J. , Ebiaku V.A. 
Published: February 2023

Phytochemicals have demonstrated considerable influence against pathogenic microbes in vitro without antimicrobial resistance. A number of studies have also reported an improved growth performance in poultry with their supplementation. In vivo antimicrobial efficacy of soursop leaf extract (SLE) was evaluated in a 49-day study on the growth performance (GP), intestinal microbes and morphology of broiler chickens. Two hundred day-old chicks (Arbor Acres) were randomly distributed to five treatments and four replicates, with ten birds each in a completely randomized design. The treatments included: CTRL (control), 5.0, 7.5, 10.0 and 12.5% SLE per litre of drinking water. Ciprofloxacin 10% was administered to the control group as antibiotics in drinking water at 1ml/2L. Feed intake (FI), weight gain (WG) and feed conversion ratio (FCR) were evaluated. Two birds per replicate were sacrificed on day 49 and eviscerated. Ileum and caeca content were collected and cultured for total coliforms, Lactobacillus, Salmonella and Escherichia coli. Mid-ileum section was cut, rinsed with distilled water and fixed in 10% formalin for evaluation of villus height and crypt depth. Analysis of variance was used for data analysis at p<0.05 confidence. Weight gain increased from 7.5% SLE while FI reduced from 10.0% and above giving the best FCR at 12.5% SLE. SLE improved WG, eliminated salmonella and reduced total coliform (1.30 to 0.00) in the ileum section. Total coliforms were eliminated, Salmonella and Escherichia colli decreased (6.02 to 1.85; 6.91 to 2.24) in the caeca section. It was concluded that SLE reduced pathogenic intestinal microbes, improved intestinal morphology and feed conversion ratio in broiler chickens.
Isolation and Characterization of Biosurfactant Producing Pseudomonas aeruginosa YLA03 and its Diesel Degradation Potentials
Authors: Arabo A.A. , Bamanga R.A. , Abdulrazak T.M. , Ahmadu J.H. , Yakasai H.M. , Fadilu M. , Shehu F.A. , Abdullahi N. 
Published: February 2023

This work was aimed at isolating and characterising biosurfactant producing bacteria strain from diesel oil contamination sites. The potential of the strain to degrade diesel oil n-alkanes was further studied. Soil samples were collected from diesel rich areas, and identified strains were grown on minimal media that contained diesel oil as only carbon source. Enrichment culture technique was used to identify bacteria. Seventeen bacteria strains were identified based on their capacity to use diesel oil as carbon source. Other qualitative screening methods were employed to determine the potential to produce biosurfactants. Isolate A03 was the only candidate that shows positive signs for drop collapse, foaming, haemolytic, oil displacement and emulsification tests. The effect of various culture parameters (incubation time, concentration of diesel, nitrogen source, pH and temperature) on biodegradation of diesel was evaluated. Optimum pH was 8.0 at 35° C, while the incubation time at maximum bacteria growth was five (5) days. The favourable diesel oil concentration was 7.5% for the isolate. The isolate has shown degradative ability towards dodecane, Tridecane, tetradecane but was unable to degrade 2, 6, 10-trimethyl- dodecane. It degraded between 10.09% - 45.39% of individual diesel alkanes. The strain has exhibited potential for degrading diesel alkanes and was identified as pseudomonas aeruginosa strain A3 (Accession Number: MZ027605) using the 16S rRNA. Sequencing.
Efforts to Improve Science Learning Outcomes and Student Activity with a Contextual Teaching and Learning Model in Grade III SDN Bangka 3 Bogor City
Authors: Widiyanti Marfie , Arief Zainal Abidin , Gatot Masitowati , Widyasari Widyasari 
Published: February 2023

This research moves from the phenomenon that occurs in the classroom that low student learning outcomes in science learning on the subject of energy and its changes. Therefore, through this research, it is hoped that it will obtain the right way to improve the quality of science learning at SDN Bangka 3 in increasing the activeness and learning outcomes of science on the subject of energy and its change by using a contextual teaching and learning model. This research aims to 1.) Describing the Contextual Teaching and Learning (CTL) Model in the process of making windmills to improve student learning outcomes about energy and its changes in science subjects in grade III SDN Bangka 3 Bogor City. 2.) Measuring how much the increase in activeness n and student learning outcomes about energy and its changes using the Contextual Teaching and Learning (CTL) Model in science subjects in grade III SDN Bangka 3 Bogor City. The results of this study show that using the Contextual Teaching and Learning Model can be a fun variety of learning for students so that it is proven to improve student learning outcomes in grade III SDN Bangka 3 Bogor City. Before using the Contextual Teaching and Learning Model, the results of the student teaching bell only reached an average score of 70 then there was an increase after using the assignment learning method to 74 in cycle I, 78 in cycle II and 82 in cycle III. From the description above, the researcher concluded that the use of the Contextual Teaching and Learning Model adapted to the learning material can create a pleasant learning situation so that there is an increase in the learning outcomes of grade III students at SDN Bangka 3 Bogor City.
Motherhood Efforts as Informal Educators in Stimulating Early Childhood Motor Development in Kedung Jaya Village, Bogor
Authors: Cahyani Inne , Efgivia Mohammad Givi , Arief Zainal Abidin , Ferdina Ferdina 
Published: February 2023

This research is a qualitative research to describe and analyze the role of mothers as informal educators in stimulating the motor development of early childhood in Kedung Jaya Village, Bogor. The problems in this study are formulated as follows: what is the role of mothers as informal educators in stimulating motor development in early childhood in Kelurahan Kedung Jaya Bogor? The method in this study uses qualitative descriptive analysis methods. The selection of data sources using purposive sampling, namely mothers with early childhood who live in Kedung Jaya Village, Bogor, totaling 3 mothers and 4 children. Data collection uses observation, interview and documentation techniques. Data analysis includes 3 phases, namely: data reduction, data presentation, conclusion drawing and verification. Checking or checking the validity of the data is carried out by triangulation, including: source triangulation and engineering triangulation. Based on the exposure and research findings, it was found that the role of mothers as informal educators in stimulating early childhood motor development has not shown ideal results. Optimizing the role of mothers as informal educators will have a major impact on early childhood motor development. The success of the mother in stimulating the motor development of the child is influenced by the age, level of education, knowledge, living environment, physical condition of the mother and the socioeconomic conditions of the family. Early childhood motor development is one of the important aspects of development for a child's future, therefore researchers recommend two important things that mothers can do in an effort to improve early childhood motor development, namely: 1) Delegating childcare to early childhood education (ECCE) institutions if the mother is working or unable to stimulate the child's motor development, 2) Providing quality time with the child.
Improving Learning Activities and Gross Motor Skills Blended Learning with the Home Visit Method
Authors: Miftahudin Asep , Arief Zainal Abidin , Wibowo Sigit , Widyasari Widyasari 
Published: February 2023

This research is a type of research (action research), aimed at collecting data on whether blended learning with the home visit method can increase learning activities and gross motor skills of group B. The formulation of the problems in this study is: 1) whether blended learning with the method home visit can improve children's gross motor skills? 2) Can blended learning with the home visit method increase children's learning activities? 3) How is the blended learning process learning with the home visit method can improve gross motor skills and learning activities of children in group B? Based on the findings of action research (action research), 1) the researchers drew the conclusion that blended learning with the home visit method can increase learning activities and gross motor skills of group B students. Students' gross motor skills in cycle 1 by 25%, cycle 2 by 30%, and in cycle 3 it increases to 75%. 2) while in student learning activities cycle 1 by 25%, cycle 2 by 40%, and in cycle 3 it increases to 80%. The blended learning process with the home visit method that has been implemented properly and proven to be the right alternative can improve the ability of children's learning activities and gross motor skills in group B. The effectiveness of blended learning can be seen from the increase in children's learning activities in each cycle and gross motor skills.
Development of the Use of Online Training in an Effort to Increase the Understanding of Educators and Education Staff towards ISO 21001: 2018 at the Kreativa School in Bogor City during the Pandemic Era
Authors: Suryani Suryani , Arief Zainal Abidin , Efgivia Mohammad Givi , Suratno Suratno 
Published: February 2023

This study aims to analyze: the procedure for developing online-based training learning with a blended learning method with a flipped classroom approach in increasing the understanding of educators and education staff to ISO 21001: 2018 at Kreativa School in the pandemic era. The problems in this study are formulated as follows: (1) What is the procedure for developing training learning in increasing the understanding of educators and education staff towards ISO 21001: 2018 in Kreativa Schools in the pandemic era?; (2) How is the feasibility of training in training to increase the understanding of educators and education staff towards ISO 21001: 2018 in Kreativa Schools in the pandemic era? ; (3) How is the effectiveness of training in training to increase the understanding of educators and education staff towards ISO 21001: 2018 in Kreativa Schools in the pandemic era? This research method is development research or also called Research and Development (RD). By using the Dick and Carey development model integrated with the PEDATI development model. The results shown in this study through the results of feasibility tests by experts, including: material experts obtained a score of 79.8 (feasible). The feasibility assessment by the media expert obtained a score of 85.8 (feasible) and the feasibility assessment by the learning design expert obtained a score of 81 (feasible). Based on the feasibility assessment of experts, it is concluded that the training learning development model is categorized as good while the results of the model effectiveness test with one to one tests with an average score of 85.75. In the small group trial, it showed an average value of 85.48 which was categorized as Good. The results of the review of the performance of the trainees obtained an N-Gain value of 85.48 which is in the range of 76 with a good category so that it can be concluded that the development of online-based training procedures for the blended learning method of the flipped classroom approach in the ISO 21001: 2018 understanding training course is able to effectively improve the learning outcomes of trainees effectively.
The Founding and Development of Secret Societies and Fraternal Orders and their Influence on Modern American Society
Authors: Massingill Ruth 
Published: February 2023

Secret societies and fraternal orders came to America with the arrival of the colonists, bringing ideals that influenced the founding of the United States. As the country grew and prospered, so did fraternal organizations, which exerted political influence, provided mutual aid for health and security to its members, and were instrumental in the growth of civic engagement. During the height of the Golden Age of Fraternity, one third of American men were members of a fraternal order. With the Depression of the 1930’s, fraternal societies began a slow decline that continues to the present although the groups evolved to became more inclusive in their membership and took on community service projects in their local communities. Scholars suggest that despite efforts to adapt to societal changes, fraternal groups, like other American civic institutions, are suffering from the growing lack of civic engagement in society, which may be traced in part to the rise of digital communication. This paper follows the evolution of fraternal organizations in the United States, explores their changing roles in society, and suggests additional research to explore the future viability of fraternal groups.
Correlation of clinical signs and magnetic resonance imaging findings in patients with lumbar spondylosis
Authors: Altan Lale , Metin Ökmen Burcu , Tuncer Tiraje , Sindel Dilşad , Fatih Çay Hasan , Hepgüler Simin , Sarıkaya Selda , Ayhan Figen , Bal Ajda , Bilgilisoy Meral , Çapkın Erhan , Cerrahoğlu Lale , Çevik Remzi , Dülgeroğlu Deniz , Durmaz Berrin , Duruöz Tuncay , Gürer Gülcan , Gürsoy Savaş , Hizmetli Sami , Kaçar Cahit , Kaptanoğlu Ece , Ecesoy Hilal , Melikoğlu Meltem , Nas Kemal , Nur Hakan , Özçakır Şüheda , Şahin Nilay , Şahin Özlem , Sarıdoğan Merih , Faruk Şendur Ömer , Sezer İlhan , Taşçı Bozbaş Gülnur , Tıkız Canan , Uğurlu Hatice 
Published: February 2023

Objectives: The purpose of the study was to contribute further to this debated topic by investigating the correlation of magnetic resonance imaging (MRI) findings with the clinical picture in lumbar spondylosis patients.
Patients and methods: This multicenter retrospective study (as part of the epidemiological project of the TLAR-OASG [Turkish League Against Rheumatism-Osteoarthritis Study Group]) included 514 patients (101 males, 413 females; mean age: 63.6±10.8 years; range, 40 to 85 years) who were diagnosed as lumbar spondylosis by clinical examination and direct X-ray between December 2016 and June 2018. Demographic characteristics of patients, Visual Analog Scale for pain, presence of radiating pain, Roland-Morris disability questionnaire, straight leg raise test, deep tendon reflexes, neurogenic intermittent claudication symptoms, any decrease of muscle strength, and abnormality of sensation were recorded. Lumbar MRI findings of the patients were recorded as positive or negative in terms of disc herniation, intervertebral disc degeneration, root compression, osteophytes, spinal stenosis. Statistical analysis was done to assess the correlation between the clinical symptoms, physical examination, and MRI findings.
Results: Correlation analysis of the MRI results and the clinical findings showed a significant correlation between straight leg raise test and root compression (p<0.001, r=0.328) and a significant correlation between neurogenic intermittent claudication and spinal stenosis (p<0.001, r=0.376). Roland-Morris disability questionnaire had a significant correlation with all MRI findings (p<0.05, r<0.200).
Conclusion: The results of this study corroborate the notion that diligent patient history and physical examination are more valuable than MRI findings, even though a higher incidence of abnormal MRI findings have been obtained in patients with disability and dermatomal radiating pain.
Towards a Green Energy Transition: REPowerEU Directive vs Environmental Acquis
Authors: Jendrośka Jerzy , Anapyanova Alina 
Published: February 2023

The article provides a brief overview and assessment of the proposed measures against the respective political and legal background. It shows that the proposed measures, while generally guided by legitimate objectives, were not properly balanced with the other EU environmental policies. In this context it claims lack of a profound debate regarding the scope and means for such balancing and considers this as a fundamental issue to be addressed before submitting any concrete measures. It then compares the proposed measures with some of the official proclamations in the Europesan Green Deal and shows issues of concern in relation to some of the concrete proposed measures. Finally, it assesses some of the proposed measures as being not controversial and presents proposals for other possible measures to be used for the purpose. The article is based on a presentation given 24th November 2022 in the context of the annual general meeting of the Environmental Law Network International.
Geriatrics emergency medicine – the need of time
Authors: Khan Naheed Habibullah , Kothari Sarah Afzal , Shahan Waheed  
Published: February 2023

Madam,
Since the inception of Emergency Medicine (EM) in Pakistan, it has been an ever-growing field, and one such current need is the development of geriatric emergency care. Older patients have a longer Emergency department (ED) stays and more frequent admissions, with an increased risk of special care and intensive care admissions. These patients have unusual presentations and multiple comorbidities, are more prone to complications, are easily affected by their surroundings and require an understanding of their social needs and thorough counselling, complicating their care. The elderly population also termed the ‘silver tsunami’ shares a significant burden of the ED in Pakistan.1 According to the 2017 national census, nearly 13.7 million people in Pakistan, were over the age of 60, accounting for roughly 7% of the total population,2 which is anticipated to double in the coming years, increasing the burden of geriatric patients. This highlights the urgent need todevelop a specialised system for the elderly in acute care settings.
Although several programmes in Pakistan focus on elderly care under Family Medicine, there remains a huge gap in emergency care, which is crucial in providing 24/7 healthcare service delivery. Geriatrics curriculum must be incorporated in emergency medicine residency programmes to facilitate patient outcomes, decrease mortality, and prevent functional decline in that age group.
The American College of Emergency Physicians (ACEP) certified hospitals as "Geriatric Emergency Departments" (GEDs) in 2018 based on GED guidelines established in 2014. The purpose was to assist in providing more effective care to the elderly population. 3 According to Kaeley et al., a considerably higher number of older people (2965, 54.9%) were hospitalised from the ED, with approximately 651, or 21.9 % admitted to the intensive care unit.4
Geriatric Emergency Medicine Fellowship training provides EDs with expertise to meet the need of a growing geriatric population. (Figure 1) These programmes can serve as a foundation for clinical innovation, research, and education focusing on the elderly population. Work on this is being done globally., Pakistan must play a role in this aspect of emergency medicine, owing to the rapidly increasing older population and advancement in GEM practice worldwide.3,5
A proper healthcare infrastructure at the government level will help geriatric patients cope with limited health resources, financial burdens, and palliative care. A better understanding of geriatric care will assist us in better allocating and utilising the limited resources we have available to alleviate the financial burden.
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