bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic improvement of economic traits in Murrah buffalo using significant SNPs from genome wide association study
Authors: GEORGE LINDA , Alex Rani , Sukhija Nidhi , Jaglan Komal , Vohra Vikas , Kumar Ravi , Verma Archana 
Published: January 2023

Abstract
GWAS helps to identify QTL and candidate genes of specific traits. Buffalo breeding mainly focused on milk production but its negative correlation with reproduction traits resulted in unfavourable decline in reproductive performance of buffalo. A genome wide scan was performed on a total of 120 Murrah buffaloes genotyped by ddRAD sequencing for 13 traits related to female fertility, production and growth. Identified 25 significant SNPs (P < 1x106) associated with Age at first calving (AFC), Age at first service (AFS), period from calving to 1st AI, Service period (SP) and 6 month body weight (6M). 15 genetic variants overlapped with different QTL regions of reported studies. Among the associated loci, outstanding candidate genes for fertility include, AQP1, TRNAE-CUC, NRIP1, CPNE4 and VOPP1 have role in different fertility traits. AQP1 gene expressed on different stages of pregnancy and in ovulatory phase. TRNAE-CUC gene related with AFC and no. of calving after 4 yrs of age. CPNE4 is glycogen content associated gene regulate muscle glycogen and upregulated in early pregnancy. NRIP1 gene have regulation over corpus luteum at pregnancy and control over ovulation and in mammary gland development. Objective to identify potential genomic regions and genetic variants associated with fertility related traits, milk production and growth traits and select most significant SNP which have positive effect on all the traits.
Methylammonium halide salt interfacial modification of perovskite quantum dots/triple-cation perovskites enable efficient solar cells
Authors: Tien Ching-Ho , Lai Hong-Ye , Chen Lung-Chien 
Published: January 2023

Abstract
Perovskite solar cells (PeSCs) have been introduced as a new photovoltaic device due to their excellent power conversion efficiency and low cost. However, due to the limitations of the perovskite film itself, the existence of defects was inevitable, which seriously affects the number and mobility of carriers in perovskite solar cells, thus restricting PeSCs Improved efficiency and stability. Here, we use methylammonium halide salts (MAX, X = Cl, Br, I) to modify the interface between perovskite quantum dots (PeQDs) film and triple-cation perovskite film, which can repair the surface defects of PeQDs film, thereby improving the crystal quality of triple-cation perovskite film. Ultimately, we achieved high short-circuit current density, high open-circuit voltage, and 20.4% power conversion efficiency in PeQDs/triple-cation PeSCs.
Higher body mass index is associated with higher hyperurcemia in patients with obesity and obstructive sleep apnea
Authors: pan na , Wang Le , Yu Zhi , Xu Chong , Wang Yan , Cao Jie , Sun Longhao , Liang Xiaoyu , Zhang Jing 
Published: January 2023

Abstract
Background
Obstructive sleep apnea (OSA) is currently recognized as a risk factor for dysmetabolism. However, the effect of OSA on serum uric acid (SUA) levels in patients with obesity remains unclear. This study aimed to investigate the association between SUA and OSA in patients with obesity and to determine whether bariatric surgery (laparoscopic sleeve gastrectomy (LSG)) could decrease SUA levels.
Methods
A total of 182 patients with obesity and OSA were recruited for this study. All patients' demographic data, polysomnography (PSG), SUA, blood glucose, lipids, and glycosylated hemoglobinA1c (HbA1c) were recorded. The patients were divided into non-hyperuricemia-OSA (NHUA-OSA, ≤ 360 µmol/L) and hyperuricemia-OSA (> 360 µmol/L) groups according to their SUA levels, and the differences between the two groups were compared and analyzed. Multiple logistic regression analysis was performed to identify the independent risk factors for hyperuricemia in obese subjects with OSA. At follow-up, changes in BMI and SUA levels before and after bariatric surgery were statistically analyzed using repeated measures analysis of variance (ANOVA).
Results
Hyperuricemia was present in 76.9% of the patients with obesity and OSA. Body mass index (BMI), oxygen desaturation index (ODI), and time spent below 90% oxygen saturation (T90%) were higher, and rapid eye movement (REM) duration during sleep and mean oxygen saturation (meanSpO2) were lower in the HUA-OSA group than in the NHUA-OSA group (all P < 0.05). Multiple logistic regression analysis showed that BMI was an independent risk factor for hyperuricemia in patients with obesity and OSA (OR 1.103, 95% CI1.032–1.178, P = 0.004) after adjusting for confounding factors. Receiver operating characteristic (ROC) curve analysis revealed that the area under the curve (AUC) for BMI was 0.695 and the optimal cutoff value was 41.80kg/m2. The BMI and prevalence of hyperuricemia gradually decreased in 23 patients reassessed at the 1, 3, and 6 months follow-ups after bariatric surgery (all P < 0.05), while the SUA level increased at 1 month and started to decrease only at 3 months after the surgery (P < 0.05).
Conclusion
BMI is a useful screening tool for detecting hyperuricemia in patients with obesity and OSA. Our results also demonstrated that bariatric surgery can effectively decrease SUA levels.
The synbiotic mixture of lactulose and Bacillus coagulans protect intestinal barrier dysfunction and apoptosis in weaned piglets challenged with lipopolysaccharide
Authors: Zheng Weijiang , Zhao Zhuyan , Yan Yunnan , Ding Liren , Yao Wen 
Published: January 2023

Abstract
Background
Lactulose has been considered to be an effective prebiotic protect intestinal mucosal injury in pigs. Bacillus coagulans (B. coagulans) is known as ‘the king of probiotics’ due to its high stability in the gastrointestinal tract, non-toxic effects, as well as high pharmacological activity. Our previous in vitro study indicating that the combination of lactulose and B. coagulans may be a good candidate as alternative of antibiotic growth promoters. However, the in vivo effects of lactulose and B. coagulans on growth and intestinal health under immune challenge in piglets remains unclear. The objective of this study is to explore the possibility of symbiotic containing lactulose and B. coagulans as alternative of antibiotic growth promoter in piglets and its effects on the intestinal mucosal injury and barrier dysfunction under immune challenge.
Methods
A total of 24 weaned male piglets were randomly assigned to 4 groups: CTR-saline, LPS-LPS, ANT-LPS and SYN-LPS groups, respectively. Piglets in the CTR-saline and LPS-LPS group were fed basal diet, while other piglets were fed either with 75 mg/kg chlortetracycline (CTC) or synbiotic mixture of 10 g/kg lactulose and 2×109 CFU/kg B. coagulans for 32 days before intraperitoneal injection of saline or lipopolysaccharide (LPS) (100 µg/kg BW), respectively. Piglets were sacrificed 4 h after LPS injection to collect samples for determining intestinal morphology, integrity and barrier functions as well as relative genes and protein expression analysis.
Results
Our data shown that dietary supplementation of synbiotic mixture of lactulose and B. coagulans had higher feed efficiency than CTC and CON. Under acute immune stress, LPS injection induced higher serum diamine oxidase activities, D-lactic acid levels, and endotoxin status, lower villus height and ratio of villus height to crypt depth, greater mRNA and lower protein expression related tight junction in both jejunum and ileum. Moreover, higher apoptosis index and higher protein expression of Bax and caspase-3 were also observed in LPS challenge group. Surprisingly, dietary synbiotic mixture with lactulose and B. coagulans remarkably protected against LPS-induced intestinal damage, barrier dysfunction and higher apoptosis progress as well as CTC.
Conclusions
Our in vivo data suggest that dietary supplementation of synbiotic mixture with lactulose and B. coagulans resulted in higher feed efficiency, and show greater resilience to LPS-induced intestinal morphology damage, barrier dysfunction and aggressive apoptosis in piglets as well as the protective effects of CTC. Those results indicating that synbiotic mixture of lactulose and B. coagulans as novel synbiotic shown beneficial effects on the performance and resilience to acute immune stress in piglets.
Study on the action mechanism of the Fujin Shengji (FJSJ) Powder on diabetic ulcer based on network pharmacology and molecular docking
Authors: Lu Xianying , Ji Wenting , Bai Dingxi , Wu Chenxin , Cai Mingjin , Wang Wei , Hou Chaoming , Gao Jing 
Published: January 2023

Abstract

Background and Objectives:Fujin Shengji (FJSJ) Powder is mainly used for treating all kinds of canker sores and gangrene. However, there are relatively few studies on the treatment of diabetic ulcer (DU) with FJSJ Powder, and the mechanism is uncertain. This study aimed to explore the potential mechanism of FJSJ Powder in the treatment of DU using network pharmacology and molecular docking.Methods:The main active ingredients and targets for the FJSJ Powder were obtained from the TCMSP database and the published reports. Diabetic ulcer-related targets were searched from the Genecards, Disgenet, Drugbank, OMIM and TTD database. The intersection targets of FJSJ Powder and diabetic ulcer were considered as potential therapeutic targets. The Metascape online database was used for the GO and KEGG enrichment analyses. And then, the protein-protein interaction (PPI) network, herb-ingredient-target-disease (H-I-T-D) network, and herb-ingredient-target-pathway (H-I-T-P) network were constructed. Finally, we used AutoDock Vina and PyMOL software to investigate the molecular docking of the top 15 key active chemical ingredients to the top 15 key target proteins in the PPI network.Result:We collect all the 800 potential targets for 100 active ingredients in FJSJ Powder, 862 diabetic ulcer-related targets, and yielded 196 potential therapeutic targets and 76 key potential therapeutic targets of the FJSJ Powder for DU treatment. A total of 2597 terms of GO enrichment analysis were obtained, including 2284 BP terms, 100 CC terms, and 213 MF terms. A total of 210 enriched KEGG pathways were identied, including AGE-RAGE, PI3K-AKT, HIF-1, MAPK, and TNF signaling pathway. The molecular docking demonstrated that boswellic acid compounds such as AKBA, ABK, α-boswellic acid, β-boswellic acid have low binding energy to several genes.Conclusions:FJSJ Powder regulates DU treatment via a multitarget, component, and signal pathway with a cooperative and bidirectional regulatory mechanism. Its active ingredients may treat DU by regulating the expression of IL6, TNF, AKT1, VEGFA, IL1B, CASP3, PPARG, TP53, and other targets, as well as activating or inhibiting AGE-RAGE, PI3K-AKT, HIF-1, MAPK, and TNF signaling pathways, which exerts immunomodulatory functions, inhibits trauma inflammation and promotes endothelial cell proliferation and migration in order to promote wound healing. This provides new insights for further experiments on the pharmacological effects of FJSJ Powder.
HAT1 regulates the immune microenvironment and promotes the malignant pathology of lower-grade gliomas
Authors: Wang Qingnan , Liu Zhendong , Cheng Xingbo , Wang Hongbo , Liang Wenjia , Zhu Qingyun , Li Pengxu , Gao Yanzheng 
Published: January 2023

Abstract
Histone acetyltransferase 1 (HAT1) is a key regulatory molecule in histone acetylation and promotes cancer malignancy. This study aimed to reveal the role of HAT1 in lower-grade gliomas (LGGs) and the potential mechanism by which it mediates pathological processes. We collected transcriptome data and detailed clinicopathological features of 906 LGG patients through TCGA RNA-seq and CGGA RNA-seq. Subsequently, various bioinformatics and molecular biology methods were used to reveal the impact of HAT1 on the prognosis, clinical characteristics, regulatory mechanism, and the potential value of antitumor immunotherapy in patients with LGG. RT-qPCR and immunohistochemistry showed that the expression level of HAT1 protein or mRNA in LGG tissue was significantly higher than that in non-tumor brain tissue. Furthermore, there is a positive expression relationship between HAT1 and clinical malignant patient characteristics such as IDH-wild and recurrent types. Kaplan–Meier and Cox regression revealed that high HAT1 expression could be used as an independent risk factor for reduced overall survival of patients. GSEA analysis showed that HAT1 overexpression promotes the activation of the cell cycle, adherens junctions, and RIG-I-like receptor signaling pathways in LGG. Finally, the TIMER database showed that HAT1 could promote the increase in multiple immune cell infiltration levels and showed a positive expression relationship with immune checkpoints in LGG. This study provides a potentially high-value target for the antitumor immunotherapy of LGG patients. For the first time, we explain the influence and mechanism of HAT1 on the prognosis of LGG patients to gain insights into the pathogenesis of LGG.
Rural-urban and socio-demographic differentials in perceived health state among aging population in Ghana.
Authors: Boateng Richard , Yawson Alfred Edwin , Adoma Prince Owusu 
Published: January 2023

Abstract
Background
The study sought to examine the effect of rural-urban residence and selected sociodemographic factors on perceived health state among aging population by employing a nationally representative data collected by the WHO from 2014 to 2015.
Methods
Data were derived from the WHO Study on Aging (SAGE) wave 2 released in 2019. A total of 4511 individuals, made up of 1018 adults between 18–49 years and 3493 respondents within the ages of 50 years and above were involved in this study. The study examined the correlations between perceived health, rural-urban residence, age, sex, marital status, ever schooled, and current work state. Subsequently, the study employed an ordinal logistic regression model to test the effect of rural-urban residence, and the demographic variables on perceived health state.
Results
The selected socio-demographic variables significantly correlated with health state, except for rural-urban residence. However, the predictive ability of rural-urban residence and the socio-demographic variables on perceived health state were strongly ascertained. An individual’s current work state (OR = 2.112) was the most significant factor that increases the perceived health state of respondents in the study.
Conclusions
Continues engagement in work-related activities and an individual’s age within the aging continuum may significantly influence the development of positive perception towards health state, which forms a vital constituent of an individual health seeking behavior.
Significance of Serial formative assessment on the teaching learning process
Authors: Sahoo Sibasish , Goswami Kalyan , Sarkar Jit , Dutta Atanu Kumar , Pal Amit , T Sasikala , Biswas Soham , M Satish 
Published: January 2023

Abstract
Introduction
Assessment is an important driving force behind an effective teaching-learning process; since assessment measures the outcome as per the objective of the education, hence it determines the entire lesson plan for apposite alignment with the curriculum. The assessment process steers the learners with a positive pull towards the desired outcome. Both the summative and formative assessments focus on how the individual student display his level of understanding.
Material & Methods
The study design was longitudinal follow-up study. The study was undertaken to find out any positive trend of performance in terms of both cognitive and psychomotor skills of students in successive formative examinations. The study included 50 medical students of first professional batch of AIIMS Kalyani from Department of Biochemistry during 2019-2020. The formative assessment tests included 3 theory examinations, 3 case-based problem-solving (CBP), 4 objectively structured practical examinations (OSPE) and 3 viva-voce, with almost regular time intervals.
Results
Both the results of the theory exams and Case based problem showed gradual significant increase in the percentage of marks from the initial assessment (p<0.01). For psychomotor domain assessment through OSPE, for almost all the classes statistically insignificant but an apparently declining trend has been observed; particularly a significant trailing trend for class 2 learners was found.
Conclusions
Competency-based curriculum needs validation through consistent assessment system. Towards this end the present study might open up new vistas for incorporation of the proper competency-based curriculum aligned with the suitable assessment process to develop an efficient medical education program.
The feasibility of existing JADAS10 cut-off values in clinical practice: A study of data from the Finnish Rheumatology Quality Register
Authors: Backström Maria , Salo Henri , Kärki Johanna , Aalto Kristiina , Rebane Katariina , Levälampi Tiina , Minna-Maija Grönlund , Kröger Liisa , Pohjankoski Heini , Hietanen Maiju , Korkatti Katja , Kuusalo Laura , Rantalaiho Vappu , Huhtakangas Johanna , Relas Heikki , Pääkkö Tero , Löyttyniemi Eliisa , Sokka-Isler Tuulikki , Vähäsalo Paula 
Published: January 2023

AbstractBackground. The ten-joint juvenile arthritis disease activity score (JADAS10) is designed to measure the level of disease activity in non-systemic juvenile idiopathic arthritis by providing a single numeric score. The JADAS10 (cJADAS10) is a modification of the JADAS10 that excludes erythrocyte sedimentation rate (ESR). Three different sets of JADAS10/cJADAS10 cut-offs for disease activity states have been published, i.e., the Backström, Consolaro, and Trincianti cut-offs. The objective of this study was to investigate the performance of existing JADAS10 cut-offs in real-life settings using patient data from the Finnish Rheumatology Quality Register (FinRheuma). Methods. Data were collected from the FinRheuma register. The proportion of patients with an active joint count (AJC) above zero when classified as being in clinically inactive disease (CID) or low disease activity (LDA) groups according to existing JADAS10/cJADAS10 cut-off levels were analyzed. Results. A significantly larger proportion of the patients classified as being in CID had an AJC > 0 when using the JADAS10/cJADAS10 cut-offs by Trincianti et al. compared to those for the other cut-offs. In the LDA group, a significantly larger proportion of the polyarticular patients (35%/29%) had an AJC of two when Trincianti JADAS10/cJADAS10 cut-offs were used compared with when Backström (11%/10%) and Consolaro (7%/3%) JADAS10/cJADAS10 cut-offs were used. Conclusions. We found the cut-offs proposed by Consolaro et al. to be the most feasible, since these cut-off levels for CID do not result in the misclassification of active disease as remission, and the proportion of patients with AJC > 1 in the LDA group is lowest using these cut-offs.
High-throughput image analysis with deep learning captures heterogeneity and spatial relationships after kidney injury
Authors: McElliott Madison C. , Al-Suraimi Anas , Telang Asha C. , Ference-Salo Jenna T. , Chowdhury Mahboob , Soofi Abdul , Dressler Gregory R. , Beamish Jeffrey A. 
Published: January 2023

Abstract
Recovery from acute kidney injury can vary widely in patients and in animal models. Immunofluorescence staining can provide spatial information about heterogeneous injury responses, but often only a fraction of stained tissue is analyzed. Deep learning can expand analysis to larger areas and sample numbers. Here we report one approach to leverage deep learning tools to quantify heterogenous responses to kidney injury that can be deployed without specialized equipment or programming expertise. We first demonstrated that deep learning models generated from small training sets accurately identified a range of stains and structures with performance similar to that of trained human observers. We then showed this approach accurately tracks the evolution of folic acid induced kidney injury in mice and highlights spatially clustered tubules that fail to repair. We then demonstrated that this approach captures the variation in recovery across a robust sample of kidneys after ischemic injury. Finally, we showed markers of failed repair after ischemic injury were correlated both spatially within and between animals and that failed repair was inversely correlated with peritubular capillary density. Combined, we demonstrate the utility and versatility of our approach to capture spatially heterogenous responses to kidney injury.
The Link Between Glycemic Control Measures and Eye Microvascular Complications in a Clinical Cohort of Type 2 Diabetes with MicroRNA-223 Signature
Authors: Da'as Sahar , Ahmed Ikhlak , Hasan Waseem , Abdelrahman Doua , Aliyev Elbay , Nisar Sabah , Bhat Ajaz Ahmed , Joglekar Mugdha , Hardikar Anand , Fakhro Khalid , Akil Ammira Al-Shabeeb 
Published: January 2023

Abstract
Background: Type 2 diabetes (T2D) is a critical healthcare challenge and priority in Qatar which is listed amongst the top 10 countries in the world, with its prevalence presently at 17% double the global average. MicroRNAs (miRNAs) are implicated in the pathogenesis of (T2D) and long-term microvascular complications including diabetic retinopathy (DR).
Methods: In this study, a T2D cohort that accurately matches the characteristics of the general population was employed to find microRNA (miRNA) signatures that are correlated with glycemic and β cell function measurements. Targeted miRNA profiling was performed in (471) T2D individuals with or without DR and (491) (non-diabetic) healthy controls from the Qatar Biobank. Discovery analysis identified 20 differentially expressed miRNAs in T2D compared to controls, of which miR-223 was significantly upregulated (fold change:5.16, p=3.6e-02) and positively correlated with glucose and hemoglobin A1c (HbA1c) levels (p-value = 9.88e-04 and 1.64e-05, respectively), but did not show any significant associations with insulin or C-peptide. Accordingly, we performed functional validation using a miR-223 mimic (overexpression) under control and hyperglycemia-induced conditions in a zebrafish model.
Results: Over-expression of miR-223 alone was associated with significantly higher glucose (42.7mg/dL, n=75 vs 38.7mg/dL, n=75, p=0.02) and degenerated retinal vasculature, and altered retinal morphology involving changes in the ganglion cell layer and inner and outer nuclear layers. Assessment of retinal angiogenesis revealed significant upregulation in the expression of vascular endothelial growth factor and its receptors, including kinase insert domain receptor. Further, the pancreatic markers, pancreatic and duodenal homeobox 1, and the insulin gene expressions were upregulated in the miR-223 group.
Conclusion: Our zebrafish model validates a novel correlation between miR-223 and DR development. Targeting miR-223 in T2D patients may serve as a promising therapeutic strategy to control DR in at-risk individuals.