bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loosening Zinc Ions from Separator Boosts Stable Zn Plating/Striping Behavior for Aqueous Zinc Ion Batteries
Authors: Zhang Yu , Liu Zeping , Li Xin , Fan Lishuang , Shuai Yong , Zhang Naiqing 
Published: September 2023

AbstractAqueous zinc ion batteries are attracting broad interest in stationary energy storage where low cost, robust security, and satisfactory capacity matter most. However, metal zinc anodes suffer from destructive dendrite issues during repeated charging/discharging process, decreasing the cycle life of batteries. Herein, smoother zinc plating/striping behaviors are achieved by group modification in the structural unit of the polymer separator. Experimental results and theoretical simulations reveal that the polymer skeleton can release zinc ion more easily when modified with carbonylation side groups and contribute to much faster zinc ion transfer capability. Typically, symmetric Zn cells with a thin thickness separator of 21 µm exhibit ultralong cycle life over 2800 h at 1 mA cm−2 within 1 mAh cm−2 and 300 h under a high current density of 5 mA cm−2 within high areal capacity of 3 mAh cm−2. Full cells using a sodium vanadium oxide cathode also show stable reversible charging/discharging performance after 2000 cycles at 3 A g−1. This work is expected to inspire promising strategies in molecular design in polymeric separator toward stabilizing metal anodes beyond this anode itself.
Refining the Substrate Surface Morphology for Achieving Efficient Inverted Perovskite Solar Cells
Authors: Guo Renjun , Wang Xi , Jia Xiangkun , Guo Xiao , Li Jia , Li Zerui , Sun Kun , Jiang Xiongzhuo , Alvianto Ezra , Shi Zhuojie , Schwartzkopf Matthias , Müller‐Buschbaum Peter , Hou Yi 
Published: September 2023

AbstractSignificant advancements in perovskite solar cells (PSCs) have been driven by the engineering of the interface between perovskite absorbers and charge transport layers. Inverted PSCs offer substantial potential with their high power conversion efficiency (PCE) and enhanced compatibility for tandem solar cell applications. Conventional hole transport materials like poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) and poly(triaryl amine) (PTAA) not only constrain the PSC efficiency but also elevate their fabrication costs. In the case of improving inverted structured PSCs according to the aforementioned concerns, utilizing self‐assembled monolayers (SAMs) as hole‐transporting layers has played a crucial role. However, the growth of self‐assembled monolayers on the substrates still limits the performance and reproducibility of inverted structured PSCs. In this study, the authors delve into the growth model of SAMs on different surface morphologies. Moreover, it is found that the plasma treatment can effectively regulate the surface morphologies of substrates and achieve conformal growth of SAMs. This treatment improves the uniformity and suppresses non‐radiative recombination at the interface, which leads to a PCE of 24.5% (stabilized at 23.5%) for inverted structured PSCs.
Variation in abundance and habitat use of the critically endangeredMicrocebus gerpiacross its fragmented range
Authors: Rakotondravony Romule , Schüßler Dominik , Rovanirina Valisoa S. T. , Ratsimbazafy Jonah , Radespiel Ute 
Published: September 2023

AbstractA link between the abundance of species and their degree of ecological specialization has previously been suggested within the primate order. Many species of lemurs were only recently described and even basic ecological data are not yet available for them. We investigated the habitat use, abundance, and habitat characteristics of the critically endangeredMicrocebus gerpiand evaluated potential impacts of vegetation structure and human disturbances on variations in its abundance. We determined abundance by systematic nocturnal surveys along 13 transects that were also used for characterizing the vegetation structure in seven study sites that were widely distributed within its range. AlthoughM. gerpioccurred in all studied lowland rainforest and littoral forest fragments in central eastern Madagascar and therefore has a higher ecological plasticity and wider distribution than previously thought, its actual Area of Occupancy is very small (339.78 km2) due to an extreme degree of habitat fragmentation throughout its range.M. gerpioccurred with a mean encounter rate of 3.04 individuals/km but abundance varied substantially between sites (0.75–4.5 individuals/km). Statistical modeling revealed that the cover of small‐ to medium‐sized trees had a positive impact on the abundance ofM. gerpi, whereas a composite disturbance score (CDS), formed on the basis of information on the prominence of fires, cattle, charcoal production and wood extraction inside and around the forest, had a negative impact on abundance. These results suggest thatM. gerpiis slightly less threatened than expected because of its larger geographic range, but also that it responds negatively to human disturbances. These findings raise strong conservation concerns and question the long‐term viability of the remaining small and isolated populations of this arboreal solitary forager.
Using the Manufacturer's Instructions for Use and Spaulding Classification System to Assess Disinfection Practices for Ultrasound Transducers in the Perioperative Setting
Authors: Phillips Bethany L. , Casanova Erica L. 
Published: September 2023

ABSTRACTProper use and disinfection of medical devices and instruments is essential to preventing infection and providing safe patient care. Because of an increase in the complexity of medical devices and patient interventions, it has become more difficult for perioperative staff members to ensure that equipment is properly used and disinfected according to the manufacturer's instructions for use and the Spaulding classification system. One type of medical device that may have multiple clinical uses is the ultrasound transducer. In the perioperative setting, ultrasound transducers can be used for surface, percutaneous, and endocavitary procedures. They also may be used at multiple times before, during, and after a procedure, which may make it challenging for perioperative staff members to know the correct disinfection practice. They should use the device's instructions for use and the Spaulding classification system to guide their clinical practice.
Selective filtration of dye by tannic acid‐molybdenum disulfide composite nanofiltration membrane
Authors: Li Yang , Jia Guozhi , Wu Lu 
Published: September 2023

AbstractDye‐selective nanofiltration membranes have great potential for chemical dye separation and purification as well as dye wastewater treatment. Two‐dimensional materials such as molybdenum disulfide (MoS2) with excellent physical and chemical properties are ideal nanofiltration membrane materials. MoS2 with few layers is used in combination with polyphenols to prepare highly selective filtration nanofiltration membranes for cationic dyes. The tannic acid (TA)‐MoS2 composite material is constructed on the surface of a micro‐porous Mixed Cellulose Ester membrane by a vacuum filtration method, and the nanofiltration membrane exhibits good flexibility and excellent film‐forming properties. The maximum filtration efficiency of TAMoS2 nanocomposite film for R6G can reach 80%, while for EY and MO, it is only 9.6% and 6.1% respectively. Filtration studies on mixtures of two different types of dyes show that the composite nanofiltration membrane has excellent separation performance. The separation characteristics and mechanism of the composite nanofilm on dyes are carefully studied, showing good selectivity for cationic dyes, which can be ascribed to the synergistic effect of non‐covalent interactions and steric hindrance. This work paves the way for the use of eco‐friendly membranes for water and wastewater treatment and provides an environmentally friendly solution for various applications.
Immobilized‐laccase bioreactors for wastewater treatment
Authors: Rodríguez‐Couto Susana 
Published: September 2023

AbstractLaccases have shown to be efficient biocatalysts for the removal of recalcitrant pollutants from wastewater. Thus, they catalyze the oxidation of a wide variety of organic compounds by reducing molecular oxygen to water. However, the use of free laccases holds several drawbacks such as poor reusability, high cost, low stability and sensitivity to different denaturing agents that may occur in wastewater. Such drawbacks can be circumvented by immobilizing laccase enzymes in/on solid carriers. Hence, during the last decades different approaches considering various techniques and solid carriers to immobilize laccase enzymes have been developed and tested for the removal of pollutants from wastewater. To scale up wastewater treatment bioprocesses, immobilized laccases are placed in different reactor configurations.
An extracellular glucose sensor for substrate‐dependent secretion and display of cellulose‐degrading enzymes
Authors: Besada‐Lombana Pamela B. , Chen Wilfred , Da Silva Nancy A. 
Published: September 2023

AbstractThe efficient hydrolysis of lignocellulosic biomass into fermentable sugars is key for viable economic production of biofuels and biorenewable chemicals from second‐generation feedstocks. Consolidated bioprocessing (CBP) combines lignocellulose saccharification and chemical production in a single step. To avoid wasting valuable resources during CBP, the selective secretion of enzymes (independent or attached to the surface) based on the carbon source available is advantageous. To enable enzyme expression and secretion based on extracellular glucose levels, we implemented a G‐protein‐coupled receptor (GPCR)‐based extracellular glucose sensor; this allows the secretion and display of cellulases in the presence of the cellulosic fraction of lignocellulose by leveraging cellobiose‐dependent signal amplification. We focused on the glucose‐responsiveness of the HXT1 promoter and engineered PHXT1 by changing its core to that of the strong promoter PTHD3, increasing extracellular enzyme activity by 81%. We then demonstrated glucose‐mediated expression and cell‐surface display of the β‐glucosidase BglI on the surface of Saccharomyces cerevisiae. The display system was further optimized by re‐directing fatty acid pools from lipid droplet synthesis toward formation of membrane precursors via knock‐out of PAH1. This resulted in an up to 4.2‐fold improvement with respect to the baseline strain. Finally, we observed cellobiose‐dependent signal amplification of the system with an increase in enzymatic activity of up to 3.1‐fold when cellobiose was added.
Enhancing the production of recombinant adeno‐associated virus in synthetic cell lines through systematic characterization
Authors: Lu Min , Lee Zion , Lin Yu‐Chieh , Irfanullah Ibrahim , Cai Wen , Hu Wei‐Shou 
Published: September 2023

AbstractRecombinant adeno‐associated virus (rAAV) is among the most commonly used in vivo gene delivery vehicles and has seen a number of successes in clinical application. Current manufacturing processes of rAAV employ multiple plasmid transfection or rely on virus infection and face challenges in scale‐up. A synthetic biology approach was taken to generate stable cell lines with integrated genetic modules, which produced rAAV upon induction albeit at a low productivity. To identify potential factors that restrained the productivity, we systematically characterized virus production kinetics through targeted quantitative proteomics and various physical assays of viral components. We demonstrated that reducing the excessive expression of gene of interest by its conditional expression greatly increased the productivity of these synthetic cell lines. Further enhancement was gained by optimizing induction profiles and alleviating proteasomal degradation of viral capsid protein by the addition of proteasome inhibitors. Altogether, these enhancements brought the productivity close to traditional multiple plasmid transfection. The rAAV produced had comparable full particle contents as those produced by conventional transient plasmid transfection. The present work exemplified the versatility of our synthetic biology‐based viral vector production platform and its potential for plasmid‐ and virus‐free rAAV manufacturing.
Standardizing cassette‐based deep mutagenesis by Golden Gate assembly
Authors: Daffern Nicolas , Francino‐Urdaniz Irene M. , Baumer Zachary T. , Whitehead Timothy A. 
Published: September 2023

AbstractProtocols for the construction of large, deeply mutagenized protein encoding libraries via Golden Gate assembly of synthetic DNA cassettes employ disparate, system‐specific methodology. Here we present a standardized Golden Gate method for building user‐defined libraries. We demonstrate that a 25 μL reaction, using 40 fmol of input DNA, can generate a library on the order of 1 × 106 members and that reaction volume or input DNA concentration can be scaled up with no losses in transformation efficiency. Such libraries can be constructed from dsDNA cassettes generated either by degenerate oligonucleotides or oligo pools. We demonstrate its real‐world effectiveness by building custom, user‐defined libraries on the order of 104–107 unique protein encoding variants for two orthogonal protein engineering systems. We include a detailed protocol and provide several general‐use destination vectors.
Mono o‐nitrobenzyl dihydrofluorescein as a photoactivatable ROS sensor for oxidative stress in live cells
Authors: Jo Hye‐Ryeong , Kim Seo Jin , Zheng Yingyu , Cho Seok‐Hyun , Nam Sang un , Moon Seungwon , Kang Chulhun , Kim Tae Woo 
Published: September 2023

AbstractThe confocal interpretation of organelle‐selective fluorogenic chemosensors can be ambiguous because of inconsistency between the sensor localization and reaction site. To overcome this limitation, we implemented the photoactivation strategy which refers to light‐controlled probe activation within cells at the desired time. In this study, we synthesized a photoactivatable dihydrofluorescein probe (1) and its cell‐permeable derivative (3, acetoxymethyl ester of 1). We confirmed their reactivity to reactive oxygen species (ROS) and photoactivation in both solution and cells. The photoactivation of probe 3 facilitated temporal control between probe treatment and the occurrence of fluorogenic events. We conducted organelle colocalization studies and cellular ROS imaging, clearly showing that the probe primarily localized to early/late endosomes and reacted with intracellular ROS at these endosomes. These findings highlight the effectiveness of the photoactivation strategy for fluorogenic ROS probes in mitigating ambiguities associated with conventional fluorogenic ROS sensing approaches.
Preliminary development and piloting of a user‐generated routine outcome measure in a children and young people's counselling service
Authors: Perry Sarah , Carpenter Simon ,   CLEAR IDEAS
Published: September 2023

AbstractBackgroundRoutine outcome monitoring has become a major focus of children and young people's mental health and counselling services in the United Kingdom, in the spirit of increasing user participation and delivering evidence‐based practice. In this pilot project, a group of young people were supported as co‐researchers to explore the ways in which counselling and therapy services measure their effectiveness.AimsTo explore young people's views of appropriate outcome measures of the effectiveness of therapy and to develop a novel user‐generated questionnaire.MethodologyThe project was informed by co‐operative enquiry and participation research, whereby the co‐researchers were also co‐participants. Eight young people designed and created a novel questionnaire to assess the effectiveness of therapy and service satisfaction. The questionnaire focused explicitly on the actual benefits of therapy to young people, as perceived by themselves and expressed in their own words. This questionnaire was subsequently piloted with 66 participants attending a specialist counselling service for children and young people who have experienced abuse.ResultsA reasonable spread of ratings to the individual items and few ‘Don't know’ responses suggested that the items were easy to understand and the rating scale functional. Total scores were positively skewed.ImplicationsAlthough there are challenges for co‐operative enquiry and meaningful participation research with young people, the limitations of current outcome evaluation require the development of more relevant and sensitive measures of children and young people's experiences of therapy.
Repurposing Salicylamides to Combat Phytopathogenic Bacteria and Induce Plant Defense Responses
Authors: Ma Yue , He Ying‐Hui , Deng Peng , Zhang Shao‐Yong , Ding Yan‐Yan , Zhang Zhi‐Jun , Zhang Bao‐Qi , An Jun‐Xia , Wang Yi‐Rong , Liu Ying‐Qian 
Published: September 2023

AbstractBased on the research strategy of “drug repurposing”, a series of derivatives and marketed drugs that containing salicylic acid skeleton were tested for their antibacterial activities against phytopathogens. Salicylic acid can not only regulate some important growth metabolism of plants, but also induce plant disease resistance. The bioassay results showed that the salicylamides exhibited excellent antibacterial activity. Especially, oxyclozanide showed the best antibacterial effect against Xanthomonas oryzae, Xanthomonas axonopodis pv. citri and Pectobacterium atroseptica with MICs of 0.78, 3.12 and 12.5 μg.mL‐1, respectively. In vivo experiments with rice bacterial leaf blight had further demonstrated that oxyclozanide exhibited stronger antibacterial activity than the commercial bactericide, thiodiazole copper. Oxyclozanide could induce plant defense responses through the determination of salicylic acid content and the activities of defense‐related enzymes including CAT, POD, and SOD in rice. The preliminarily antibacterial mechanism study indicated that oxyclozanide exhibited the antibacterial activity by disrupting cell integrity and reducing bacterial pathogenicity. Additionally, oxyclozanide could induce plant defense responses through the determination of salicylic acid content.
A New 3‐Benzylphthalide from the Moss Erythrodontium julaceum
Authors: Khanh Van Nguyen Ngoc , Aree Thammarat , Quynh Trang Nguyen Thi , Ngoc Thao Nguyen Thi , Tri Nguyen Huu , Duong Thuc‐Huy , Nguyen Ngoc‐Hong , Kim Tuyen Pham Nguyen 
Published: September 2023

AbstractFrom the moss Erythrodontium julaceum Paris growing in Vietnam, julacelide (1), a new 3‐benzylphthalide, along with methyl orsellinate (2), ethyl orsellinate (3), 4‐O‐methylhaematommic acid (4), and zeorin (5), were isolated and structurally elucidated. Their chemical structures were elucidated through extensive 1D and 2D NMR analysis and high‐resolution mass spectroscopy as well as through comparisons to the existing literature. Compound 4‐O‐methylhaematommic acid was a new natural product. The absolute configuration of julacelide was defined using time‐dependent density functional theory (TDDFT) calculations. Julacelide was evaluated for α‐glucosidase inhibition.
cFLIPL alleviates myocardial ischemia‐reperfusion injury by regulating pyroptosis
Authors: Zhang Dong , Wu Hui , Liu Di , Ye Ming , Li Yunzhao , Zhou Gang , Yang QingZhuo , Liu YanFang , Li Yi 
Published: September 2023

AbstractAlleviating myocardial ischemia‐reperfusion injury (MIRI) plays a critical role in the prognosis and improvement of cardiac function following acute myocardial infarction. Pyroptosis is a newly identified form of cell death that has been implicated in the regulation of MIRI. In our study, H9c2 cells and SD rats were transfected using a recombinant adenovirus vector carrying cFLIPL, and the transfection was conducted for 3 days. Subsequently, H9c2 cells were subjected to 4 h of hypoxia followed by 12 h of reoxygenation to simulate an in vitro ischemia‐reperfusion model. SD rats underwent 30 min of ischemia followed by 2 h of reperfusion to establish an MIRI model. Our findings revealed a notable decrease in cFLIPL expression in response to ischemia/reperfusion (I/R) and hypoxia/reoxygenation (H/R) injuries. Overexpression of cFLIPL can inhibit pyroptosis, reducing myocardial infarction area in vivo, and enhancing H9c2 cell viability in vitro. I/R and H/R injuries induced the upregulation of ASC, cleaved Caspase 1, NLRP3, GSDMD‐N, IL‐1β, and IL‐18 proteins, promoting cell apoptosis. Our research indicates that cFLIPL may suppress pyroptosis by strategically binding with Caspase 1, inhibiting the release of inflammatory cytokines and preventing cell membrane rupture. Therefore, cFLIPL could potentially serve as a promising target for alleviating MIRI by suppressing the pyroptotic pathway.
May‐Thurner syndrome due to abdominal aortic aneurysm compression
Authors: Ono Ryohei , Kobayashi Yoshio 
Published: September 2023

Key Clinical MessageMay‐Thurner syndrome (MTS) can be caused by abdominal aortic aneurysm (AAA) compressing the left common iliac vein against the lumbar vertebra. We reported a rare case of MTS due to AAA Compression successfully treated with anticoagulation.
Delayed diagnosis of hydrocephalus: Negligence of enlarging head in a child by parents
Authors: Khizar Ahtesham , Zahid Soha , Yadav Pradhumna Kumar 
Published: September 2023

AbstractA 1‐year‐old girl presented with an overly enlarged head for 5 months. Negligence of parents regarding treatment for this enlarged head is concerning. Early treatment can avoid a lot of complications. Hydrocephalus secondary to aqueductal stenosis was diagnosed after a thorough history, examination, and investigations. Endoscopic third ventriculostomy was performed.
Anesthesia using remimazolam during coronary artery bypass surgery in a patient with decreased left ventricular function
Authors: Narumi Shingo , Ishida Yusuke , Igarashi Sae , Sekiguchi Shunya , Kawachi Aya , Tomino Mikiko 
Published: September 2023

Key Clinical MessageRemimazolam is a new benzodiazepine sedative and has the characteristic of causing minimal effects on circulation. This case indicates that it can be considered as an option for anesthesia management of patients with decreased cardiac function.AbstractSome patients who undergo cardiac surgery have reduced cardiac function, which can often make anesthesia management difficult owing to severe hypotension at the time of anesthesia induction. Therefore, it is important to select drugs that cause minimal circulatory depression. On the other hand, in 2020, the use of remimazolam, a short‐acting benzodiazepine sedative, was approved in Japan, and reports of its use in various patients have been increasing. This drug has the characteristic of causing minimal effects on circulation. We here report the safe use of remimazolam in the anesthesia management of a patient with decreased cardiac function who was diagnosed as having angina pectoris. The patient was a 73‐year‐old man scheduled for coronary artery bypass graft (CABG) surgery. Remimazolam was used for sedation purposes during anesthesia induction. During surgery, there were no significant hemodynamic changes and the patient remained in stable cardiovascular condition. Our present case indicates that remimazolam can be considered as an option for anesthesia management in CABG for patients with decreased cardiac function.