bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transformative systemic changes to embed environmental sustainability in foodservices: A grounded theory exploration
Authors: Smeltzer Madison E. , Davidson Alexandra R. , Reidlinger D. P. , MacKenzie‐Shalders Kristen L. 
Published: March 2023

AbstractAimsFoodservices are a strong contributor to global environmental impact. Systemic change is required to lead the transformation towards environmentally sustainable foodservices. However, guidance to support foodservices to be more environmentally sustainable is lacking. The aim was to explore food‐related environmentally sustainable strategies and their transferability to foodservices in a range of settings, to inform a framework for future application and research.MethodsA constructivist grounded theory study design was used. Semi‐structured interviews with foodservice sustainability consultants, who support foodservice organisations to improve environmental sustainability, were conducted. Interviews were recorded, transcribed, and coded line‐by‐line. Ten consultants were purposively sampled for diversity in location, organisation type, funding model, and services provided. Codes were collapsed into categories, to inform the development of themes and a framework for the implementation of strategies.ResultsFour sub‐themes were created under an overarching theme of ‘Transforming the Foodservice System’: embedding leadership, shifting perspective, constructing collaborative networks, and fostering momentum. A range of implementation strategies were captured within the sub‐themes.ConclusionThese themes informed the development of a practical application framework for implementing sustainable strategies in foodservices that is useful for practice and future research in the area.
Delineation of a lactococcal conjugation system reveals a restriction‐modification evasion system
Authors: Ortiz Charneco Guillermo , Kelleher Philip , Buivydas Andrius , Dashko Sofia , de Waal Paul P. , van Peij Noël N. M. E. , Roberts Richard J. , Mahony Jennifer , van Sinderen Douwe 
Published: March 2023

AbstractPlasmid pUC11B is a 49.3‐kb plasmid harboured by the fermented meat isolate Lactococcus lactis subsp. lactis UC11. Among other features, pUC11B encodes a pMRC01‐like conjugation system and tetracycline‐resistance. In this study, we demonstrate that this plasmid can be conjugated at high frequencies to recipient strains. Mutational analysis of the 22 genes encompassing the presumed pUC11B conjugation cluster revealed the presence of several genes with essential conjugation functions, as well as a gene, trsR, encoding a putative transcriptional repressor of this conjugation cluster. Furthermore, plasmid pUC11B encodes an anti‐restriction protein, TrsAR, which facilitates higher conjugation frequencies when pUC11B is transferred into recipient strains containing Type II or Type III RM systems. These findings demonstrate how RM mechanisms can be circumvented when they act as a biological barrier for conjugation events.
β‐Cell glucokinase expression was increased in type 2 diabetes subjects with better glycemic control
Authors: Liu Jingwen , Fu Hui , Kang Fuyun , Ning Guang , Ni Qicheng , Wang Weiqing , Wang Qidi 
Published: March 2023

AbstractBackgroundType 2 diabetes (T2D) is characterized by a progressive deterioration of β‐cell function with a continuous decline in insulin secretion. Glucokinase (GCK) facilitates the rate‐limiting step of glycolysis in pancreatic β‐cells, to acquire the proper glucose‐stimulated insulin secretion. Multiple glucokinase activators (GKAs) have been developed and clinically tested. However, the dynamic change of human pancreatic GCK expression during T2D progression has not been investigated.MethodsWe evaluated GCK expression by measuring the average immunoreactivity of GCK in insulin+ or glucagon+ cells from pancreatic sections of 11 nondiabetic subjects (ND), 10 subjects with impaired fasting glucose (IFG), 9 with well‐controlled T2D (wT2D), and 5 individuals with poorly controlled T2D (uT2D). We also assessed the relationship between GCK expression and adaptive unfolded protein response (UPR) in human diabetic β‐cells.ResultsWe did not detect changes of GCK expression in IFG islets. However, we found β‐cell GCK levels were significantly increased in T2D with adequate glucose control (wT2D) but not in T2D with poor glucose control (uT2D). Furthermore, there was a strong positive correlation between GCK expression and adaptive UPR (spliced X‐box binding protein 1 [XBP1s] and activating transcription factor 4 [ATF4]), as well as functional maturity marker (urocortin‐3 [UCN3]) in human diabetic β‐cells.ConclusionsOur study demonstrates that inductions of GCK enhanced adaptive UPR and UCN3 in human β‐cells, which might be an adaptive mechanism during T2D progression. This finding provides a rationale for exploring novel molecules that activate β‐cell GCK and thereby improve pharmacological treatment of T2D.
First‐degree relatives of patients with systemic lupus erythematosus: Autoreactivity but not autoimmunity?
Authors: Penev Bogdan , Vasilev Georgi , Todorova Ekaterina Ivanova , Tumangelova‐Yuzeir Kalina , Kurteva Ekaterina , Monov Simeon , Kyurkchiev Dobroslav 
Published: March 2023

AbstractBackgroundSystemic lupus erythematosus (SLE) is a disorder with a complex immunopathogenesis. It is well known that the disease begins with immunological alterations and autoantibody appearance in the serum years before clinical onset. As SLE has a strong tendency to familial aggregation, first‐degree relatives (FDRs) constitute a group at elevated risk. The current understanding is that external risk factors trigger underlying immune dysregulations, leading to overt disease in those with elevated genetic risk.ObjectiveThis cross‐sectional study investigates the degree to which clinical features, external risk factors, and immunological profiles differ in SLE FDRs from healthy individuals and SLE patientts.MethodsThree groups were studied: Lupus patient FDRs (n = 56), healthy controls (n = 20), and SLE patients (n = 20). FDRs and healthy participants completed a detailed clinical questionnaire that included questions regarding smoking and estrogen drug history. All participants were tested for the presence of the following antinuclear autoantibodies (ANAs) against: nRNP/Sm, Sm, Ro60, Ro‐52, La, Scl‐70, PM‐Scl, PM‐ Scl, Jo‐1, CENP B, PCNA, dsDNA, nucleosomes, histones, RibP, AMA M2, DFS70, and eight soluble cytokines, including transforming growth factor‐β (TGF‐β), vitamin D levels, and antibodies against Epstein‐Barr virus (EBV).ResultsCompared with the healthy controls, FDRs had higher titers of ANA, more specific staining immunofluorescent patterns, and more autoantibody specificities. Furthermore, FDRs differed significantly in their TGF‐β levels from the other two groups. In FDRs, some clinical features (hair loss, skin, and oral ulcer‐like lesions) were associated with higher ANA titers and some (oral ulcer‐like lesions) with the anti‐Ro60‐specific antibody. Interestingly, there was an association between ANA titers and levels of antibodies against EBV only in the FDR group.ConclusionFirst‐degree relatives display unique clinical and immunological profiles, placing them between healthy individuals and SLE patients, with a balance between compensated immune dysregulation and disease‐developing potential. A possible association between ANA titer and the number of clinical complaints is observed, which needs to be confirmed in more extensive studies.
Public health clauses in international investment agreements: Sword or shield?
Authors: Thow Anne Marie , Alschner Wolfgang , Aljunied Faisal 
Published: March 2023

AbstractThe interaction of international investment agreements and public health is marked by ambivalence. Investment treaties can help attract investment into the health sector but also enable international legal claims by foreign investors against public health measures. This paper seeks to better understand their relationship by systematically mapping the purpose of health inclusions in bilateral investment treaties (BITs) between 1959 and 2021. We find that health‐related clauses, present in 18% of all BITs, are used both as a shield and a sword. Most health mentions protect host countries from claims against public health measures, yet an increasing number of recent BITs, primarily from developing countries, also pursue an offensive public health agenda by advancing health safety at work and health‐related corporate social responsibility of investors.
Longitudinal Incremental Propensity Score Interventions for Limited Resource Settings
Authors: Sarvet Aaron L. , Wanis Kerollos N. , Young Jessica G. , Hernandez-Alejandro Roberto , Stensrud Mats J. 
Published: March 2023

Abstract
Many real-life treatments are of limited supply and cannot be provided to all individuals in the population. For example, patients on the liver transplant waiting list usually cannot be assigned a liver transplant immediately at the time they reach highest priority because a suitable organ is not immediately available. In settings with limited supply, investigators are often interested in the effects of treatment strategies in which a limited proportion of patients receive an organ at a given time, that is, treatment regimes satisfying resource constraints. Here, we describe an estimand that allows us to define causal effects of treatment strategies that satisfy resource constraints: incremental propensity score interventions (IPSIs) for limited resources. IPSIs flexibly constrain time-varying resource utilization through proportional scaling of patients' natural propensities for treatment, thereby preserving existing propensity rank ordering compared to the status quo. We derive a simple class of inverse-probability-weighted estimators, and we apply one such estimator to evaluate the effect of restricting or expanding utilization of “increased risk” liver organs to treat patients with end-stage liver disease.
Potent antitumor effects of the conditioned medium of bone marrow‐derived mesenchymal stem cells via IGFBP‐4
Authors: Furusaka Yuma , Inoue Shinya , Mizoguchi Izuru , Hasegawa Hideaki , Katahira Yasuhiro , Watanabe Aruma , Sakamoto Eri , Sekine Ami , Miyakawa Satomi , Umezu Tomohiro , Owaki Toshiyuki , Yoneto Toshihiko , Yoshimoto Takayuki 
Published: March 2023

AbstractCell transfer therapy using mesenchymal stem cells (MSCs) has pronounced therapeutic potential, but concerns remain about immune rejection, emboli formation, and promotion of tumor progression. Because the mode of action of MSCs highly relies on their paracrine effects through secretion of bioactive molecules, cell‐free therapy using the conditioned medium (CM) of MSCs is an attractive option. However, the effects of MSC‐CM on tumor progression have not been fully elucidated. Herein, we addressed this issue and investigated the possible underlying molecular mechanisms. The CM of MSCs derived from human bone marrow greatly inhibited the in vitro growth of several human tumor cell lines and the in vivo growth of the SCCVII murine squamous cell carcinoma cell line with reduced neovascularization. Exosomes in the MSC‐CM were only partially involved in the inhibitory effects. The CM contained a variety of cytokines including insulin‐like growth factor binding proteins (IGFBPs). Among them, IGFBP‐4 greatly inhibited the in vitro growth of these tumors and angiogenesis, and immunodepletion of IGFBP‐4 from the CM significantly reversed these effects. Of note, the CM greatly reduced the phosphorylation of AKT, ERK, IGF‐1 receptor beta, and p38 MAPK in a partly IGFBP4‐dependent manner, possibly through its binding to IGF‐1/2 and blocking the signaling. The CM depleted of IGFBP‐4 also reversed the inhibitory effects on in vivo tumor growth and neovascularization. Thus, MSC‐CM has potent inhibitory effects on tumor growth and neovascularization in an IGFBP4‐dependent manner, suggesting that cell‐free therapy using MSC‐CM could be a safer promising alternative for even cancer patients.
Dihydroorotate dehydrogenase regulates ferroptosis in neurons after spinal cord injury via the P53‐ALOX15 signaling pathway
Authors: Li Dachuan , Lu Xiao , Xu Guangyu , Liu Siyang , Gong Zhaoyang , Lu Feizhou , Xia Xinlei , Jiang Jianyuan , Wang Hongli , Zou Fei , Ma Xiaosheng 
Published: March 2023

AbstractBackgroundSpinal cord injury (SCI) is a highly disabling condition in spinal surgery that leads to neuronal damage and secondary inflammation. Ferroptosis is a non‐apoptotic type of cell death that has only recently been identified, which is marked primarily by iron‐dependent and lipid‐derived reactive oxygen species accumulation, and accompanied by morphological modifications such as mitochondrial atrophy and increase in membrane density. Dihydroorotate dehydrogenase (DHODH) is a powerful inhibitor of ferroptosis and has been demonstrated to inhibit cellular ferroptosis in tumor cells, but whether it can inhibit neuronal injury following spinal cord injury remains ambiguous.MethodsIn this study, the effect of DHODH on neuronal ferroptosis was observed in vivo and in vitro using a rat spinal cord injury model and erastin‐induced PC12 cells, respectively. A combination of molecular and histological approaches was performed to assess ferroptosis and explore the possible mechanisms in vivo and in vitro.ResultsFirst, we confirmed the existence of neuronal ferroptosis after spinal cord injury and that DHODH attenuates neuronal damage after spinal cord injury. Second, we showed molecular evidence that DHODH inhibits the activation of ferroptosis‐related molecules and reduces lipid peroxide production and mitochondrial damage, thereby reducing neuronal ferroptosis. Further analysis suggests that P53/ALOX15 may be one of the mechanisms regulated by DHODH. Importantly, we determined that DHODH inhibits ALOX15 expression by inhibiting P53.ConclusionsOur findings reveal a novel function for DHODH in neuronal ferroptosis after spinal cord injury, suggesting a unique therapeutic target to alleviate the disease process of spinal cord injury.
A Manifesto for project management research
Authors: Locatelli Giorgio , Ika Lavagnon , Drouin Nathalie , Müller Ralf , Huemann Martina , Söderlund Jonas , Geraldi Joana , Clegg Stewart 
Published: March 2023

AbstractProject management research has evolved over the past five decades and is now a mature disciplinary field investigating phenomena of interest to academics, practitioners and policymakers. Studies of projects and project management practices are theoretically rich and scientifically rigorous. They are practically relevant and impactful when addressing the pursuit of operational, tactical and strategic advancements in the world of organisations. We want to broaden the conversation between project management scholars and other scholars from cognate disciplines, particularly business and management, in a true scholarship of integration and cross‐fertilisation. This Manifesto invites the latter scholars to join efforts providing a foundation for further creative, theoretical and empirical contributions, including but not limited to tackling grand challenges such as climate change, pandemics, and global poverty. To this end, we identify five theses:Projects are often ‘agents of change’ and hence fundamental to driving the innovation and change required to tackle grand challenges.Much project management research leverages and challenges theories across disciplines, including business, organisation and management studies, contributing to developing new theories, including those specific to projects and temporary organisations.‘Projects’ are useful units of analysis, project management research is ideal for scientific cross‐fertilisation and project management scholars welcome academics from other communities to engage in fruitful conversations.As in many other fields of knowledge, the project management research community embraces diversity, welcoming researchers of different genders and various scientific and social backgrounds.Historically rooted in ‘problem‐solving’ and normative studies, project management research has become open to interpretative and emancipatory research, providing opportunities for other business, management and organisational scholars to advance their knowledge communities.
Novel partial deletions, frameshift and missense mutations of CSF1R in patents with CSF1R‐related leukoencephalopathy
Authors: Ishiguro Takanobu , Konno Takuya , Hara Norikazu , Zhu Bin , Okada Satoshi , Shibata Mamoru , Saika Reiko , Kitano Takaya , Toko Megumi , Nezu Tomohisa , Hama Yuka , Kawazoe Tomoya , Takahashi‐Iwata Ikuko , Yabe Ichiro , Sato Kota , Takeda Hayato , Toda Shintaro , Nishimiya Jin , Teduka Toshiyuki , Nozaki Hiroaki , Kasuga Kensaku , Miyashita Akinori , Onodera Osamu , Ikeuchi Takeshi 
Published: March 2023

AbstractBackground and purposeColony‐stimulating factor 1 receptor (CSF1R)‐related leukoencephalopathy is an adult‐onset leukoencephalopathy caused by mutations in CSF1R. The present study aimed to explore the broader genetic spectrum of CSF1R‐related leukoencephalopathy in association with clinical and imaging features.MethodsMutational analysis of CSF1R was performed for 100 consecutive patients with adult‐onset leukoencephalopathy. Sequence and copy number variation (CNV) analyses of CSF1R were performed. The genomic ranges of the deletions were determined by long‐read sequencing. Ligand‐dependent autophosphorylation of CSF1R was examined in cells expressing the CSF1R mutants identified in this study.ResultsCSF1R mutations were identified in 15 patients, accounting for 15% of the adult‐onset leukoencephalopathy cases. Seven novel and five previously reported CSF1R mutations were identified. The novel mutations, including three missense and one in‐frame 3 bp deletion, were located in the tyrosine kinase domain (TKD) of CSF1R. Functional assays revealed that none of the novel mutations in the TKD showed autophosphorylation of CSF1R. Two partial deletions of CSF1R were identified that resulted in lack of the C‐terminal region, including the distal TKD, in two patients. Various clinical features including cognitive impairment, psychiatric symptoms and gait disturbance were observed. Various degrees of the white matter lesions and corpus callosum abnormalities on magnetic resonance imaging and characteristic calcifications on computed tomography were observed as imaging features.ConclusionsOur results highlight the importance of examining the CNV of CSF1R even when Sanger or exome sequencing reveals no CSF1R mutations. Genetic examination of sequences and CNV analyses of CSF1R are recommended for an accurate diagnosis of CSF1R‐related leukoencephalopathy.
Do different platforms of coronavirus vaccines have different impacts on psoriasis flares?
Authors: Chularojanamontri Leena , Likittanasombat Surachanee , Chaiyabutr Chayada , Silpa‐archa Narumol , Wongpraparut Chanisada 
Published: March 2023

AbstractThis study evaluated the impacts on psoriasis flares of 3 vaccine platforms: inactivated, viral vector and mRNA. Respectively, 198 and 96 psoriasis patients with and without COVID‐19 vaccination during the study period. Group comparison revealed no increased risk of psoriasis flaring after COVID‐19 vaccination. The vaccinated group received 425 doses of vaccine (140 inactivated, 230 viral vector and 55 mRNA). Patients' self‐reported symptoms included all three platforms causing psoriasis flare, but the highest was among patients administered with mRNA vaccines. Most flares were mild to moderate, and most patients (89.8%) managed their flare‐up lesions without rescue therapy. In conclusion, our study showed that the rate of psoriasis flare was not significantly different between vaccinated and unvaccinated groups. Factors that might explain psoriasis flare include vaccine‐related psychological stress and side effects from vaccination. Different platforms of corona vaccines seemed to have different impact of psoriasis flares. Based on our results and the recommendations of several consensus guidelines, the benefits of COVID vaccinations outweigh the risks to patients with psoriasis. Patients with psoriasis should receive a COVID vaccine as soon as one is available.
Nanoindentation characterization on competing propagation between the transgranular and intergranular cracking of 316H steel under creep‐fatigue loading
Authors: Song Yuxuan , Pan Zhouxin , Yu Ting , Zhou Shuiqing , Li Yuebing , Jin Weiya , Gao Zengliang , Ma Yi 
Published: March 2023

Abstract316H austenitic stainless is regarded as an excellent candidate in the most advanced generation of nuclear reactor at elevated temperature. The effects of dwell times and strain amplitudes on the creep‐fatigue (CF) fracture mechanism of 316H steel were studied by using nanoindentation. The cyclic lives are observed to decrease with increasing strain amplitudes, while they are insensitive to the variation in dwell times. In comparison to the as‐received specimen, the nano‐hardness was evidently enhanced in both the fracture and interior regions for the CF‐tested one, whereas elastic modulus was little changed. As related to the creep resistance, creep flow could be suppressed with increasing strain amplitudes and dwell times, especially for the fracture edge. And the correlation between strain rate sensitivity (m) during creep flow and CF testing conditions was obtained. Based on the morphologies of fracture surface and variation in H, E, and m, a normalized damage indicator ln(H/E)/m is proposed to characterize the competing propagation of the transgranular (fatigue damage) and intergranular (creep damage) cracking in the CF fracture.
Fatigue properties of resistance spot‐welded maraging steel produced by selective laser melting
Authors: Shi Yandong , Luo Cheng , Li Wenkai , Shi Liting , Zhang Yansong , Su Xuming 
Published: March 2023

AbstractThis research study investigates the fatigue properties of resistance spot welds (RSWs) made from selective laser‐melted (SLMed) maraging steel (MS1) and compares them with dual‐phase (DP) steel RSWs. The findings reveal that MS1 spot welds exhibit superior fatigue properties compared with DP steel RSWs, regardless of the printing orientation of the MS1 substrate. Furthermore, fatigue fracture occurs at the MS1 substrate, with a transition of fracture location observed from MS1 to itself and MS1‐to‐DP600 dissimilar welds. The results of structural stress analysis indicate that the weld nugget diameter is not the only factor affecting the fatigue life. The study also highlights the importance of considering materials inhomogeneity and fracture modes in fatigue life assessment. Based on the findings, the study recommends the development of a new fatigue life prediction curve for spot welds of additive manufacturing (AM) materials. Such a curve would be beneficial in promoting the further application of AM technology for functional parts of design verification vehicles (DCV) and production cars. Overall, the research contributes to the understanding of the fatigue properties of RSWs made from SLMed MS1 and DP steel, which has implications for the design and manufacturing of parts in the automotive industry.