bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design, Synthesis and Cytotoxicity of New Coumarin‐1,2,3‐triazole Derivatives: Evaluation of Anticancer Activity and Molecular Docking Studies
Authors: Premsagar Miriyala Victor , Raj Thommandru Prakash , Kashanna Jajula , Govinda Varadhi , Ravi Guguloth , Kishore Ravada 
Published: May 2023

AbstractA library of new coumarin‐1,2,3‐triazole hybrids 7a–l were synthesized from 4‐(diethylamino)‐2‐hydroxybenzaldehyde precursor through a series of reactions including Vilsmeier‐Haack reaction and condensation reaction to achieve key intermediate oxime and further performed click reaction by using different aromatic azides. We screened all molecules in silico against crystal structure of Serine/threonine‐protein kinase 24 (MST3), based on these results all molecules were screened for their cytotoxicity against human breast cancer MCF‐7 and lung cancer A‐549 cell lines. Compound 7 b (p‐bromo) showed best activity against both cell lines MCF‐7 and A‐549 with IC50 value of 29.32 and 21.03 μM, respectively, in comparison to Doxorubicin corresponding IC50 value of 28.76 and 20.82 μM. Another compound 7 f (o‐methoxy) also indicated good activity against both cell lines with IC50 value of 29.26 and 22.41 μM. The toxicity of all compounds tested against normal HEK‐293 cell lines have not shown any adverse effects.
Imaging findings of superficial angiomyxoma in the eye socket
Authors: Chen Guiwu , Liu Wenqin , Cao Yongjie , Guo Xiaodong , Xie Yuhuan 
Published: May 2023

Key Clinical MessageA 23‐year‐old male with a tumor in the eye socket was characterized by multimodal images, including ultrasonography, computed tomography, and magnetic resonance imaging. After admission, surgical resection of the tumor was performed and superficial angiomyxoma was confirmed. Two years later, this tumor recurred in the same location.AbstractSuperficial angiomyxoma (SAM) is a rare benign neoplasm composed mostly of myxoid material that can affect many parts of the body in middle‐aged patients. Only a few case reports have involved imaging, which is extremely insufficient. Here, we present a case of SAM in the eye socket evaluated by imaging, including ultrasonography, computed tomography, and magnetic resonance imaging. The patient underwent surgical resection, and the diagnosis of SAM was confirmed. During the postoperative follow‐up, the tumor recurred in the same location without metastasis 2 years later.
Tuning the photophysical and chiroptical properties of [4]helicene‐diketopyrrolopyrroles
Authors: Mastropasqua Talamo Maurizio , Cauchy Thomas , Zinna Francesco , Pop Flavia , Avarvari Narcis 
Published: May 2023

AbstractSynthesis and functionalization of diketopyrrolo[3,4‐c]pyrrole (DPP) derivatives containing chiral groups able to induce a strong chiral perturbation of the DPP core are still a challenging task. We report in this work the straightforward preparation of four bis([4]helicene)‐DPP and bis([4]thiahelicene)‐DPP dyes upon the condensation of 2‐CN‐[4](thia)helicene precursors, followed by their N‐alkylation by nucleophilic substitution (compounds 9–11) or by a Mitsunobu‐type strategy (compound 12). Compound 12, which contains sec‐phenylethyl groups attached to the nitrogen atoms, has been obtained as (R,R) and (S,S) enantiomers. The four DPP‐helicenes are luminescent in solution, while the N‐benzyl (10) and N‐sec‐phenethyl (12) are emissive in the solid state as well. The chiroptical properties of compound 12 in solution and in the solid state indicate a strong chiral perturbation provided by the α‐stereogenic centres, in spite of the stereodynamic nature of the [4]helicene flanking units.
Speciation and Photoluminescent Properties of a 2,6‐Bis(pyrrol‐2‐yl)pyridine in Three Protonation States
Authors: Leary Dylan C. , Martinez Jordan C. , Gowda Anitha S. , Akhmedov Novruz G. , Petersen Jeffrey L. , Milsmann Carsten 
Published: May 2023

Abstract2,6‐Bis(pyrrol‐2‐yl)pyridines are important building blocks for supramolecular assemblies and photoluminescent main group and transition metal compounds. Sterically encumbered 2,6‐bis(5‐(2,4,6‐trimethylphenyl)‐3‐phenyl‐1H‐pyrrol‐2‐yl)pyridine, H2MesPDPPh, can adopt monomeric and dimeric structures in the solid state and in solution, controlled by competing inter‐ and intramolecular hydrogen bonds. Deprotonation in the presence of lithium cations provides Li2MesPDPPh, which can be isolated in monomeric and dimeric forms depending on solvent polarity. Protonation of H2MesPDPPh disrupts intramolecular hydrogen bonding and provides the monomeric pyridinium salt [H3MesPDPPh]Cl. Independent of solvent polarity, all three derivatives exhibit intense fluorescence in solution. The absorption and emission spectra are highly sensitive to the level of protonation, which can be rationalized by the effects of (de)protonation on the HOMO and LUMO of the tricyclic π‐system.
Transformation and Mutagenesis of Chlamydia trachomatis and C. muridarum Utilizing pKW Vector
Authors: Wolf Katerina 
Published: May 2023

AbstractA gene deletion by allelic exchange via homologous recombination from a bacterial genome represents a valuable genetic tool for studying a role(s) of determinants involved in various aspects of pathogenesis. Due to chlamydial obligate intracellular lifestyle and comparatively low transformation rate, the mutagenesis of Chlamydia utilizes types of suicide vectors that have to be maintained and propagated by the bacteria throughout several rounds of their intracellular developmental cycle. These deletion constructs must be lost by chlamydiae once null mutant formation is achieved. pKW is a small, 5.45 bp, pUC19‐derived vector, which has been recently successfully employed for the generation of deletion mutants in C. trachomatis, serovariant D, and C. muridarum. This vector contains both, E. coli as well as chlamydial species‐specific plasmid origins of replication, allowing for its propagation by both bacterial genera under a selective pressure. However, once the selective antibiotic is removed from culture, chlamydiae rapidly lose pKW, and the subsequent reintroduction of the selective antibiotic back to chlamydiae‐infected cells results efficiently in the selection of generated deletion mutants. Protocols provided here describe in detail the preparation of pKW deletion constructs for C. trachomatis and C. muridarum applicable for chlamydial transformation and the production of null mutant in non‐essential genes. Protocols provided here, are describing in detail methods for assembly of the pKW shuttle vector and generation of deletion mutants in C. trachomatis and C. muridarum. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Assembly of pKW shuttle vectorBasic Protocol 2: Generation of a deletion mutant in C. trachomatis, serovars D and L2 and, Chlamydia muridarumSupport Protocol: Transformation of C. trachomatis, serovars B
Z number improved reference ideal method‐based decision‐making process for enforcing cooperation during data dissemination in mobile ad hoc networks
Authors: Mylsamy Rajkumar , Jayaprakash Karthika 
Published: May 2023

SummaryIn mobile ad hoc networks (MANETs), cooperative communication and resource constraint are the two important core characteristics essential to guarantee trusted data dissemination. The cooperative communication between mobile nodes depends on the trust rendered by them towards the process of reliable data routing. However, stringent resource constraints of mobile nodes such as energy, memory, communications, and computations result in the introduction of selfish and malicious node that completely degrades the network performance in different dimensions. In this paper, Z number improved reference ideal method (RIM)‐based decision‐making process (NIRIMDMP) is proposed with the merits of maximizing deviation method (MDM) and best–worst method (BWM) to ensure reliable data routing by modeling the cooperation degree in terms of Z number. This NIRIMDMP adopted Z number to represent the information reliability and handle the problem of inherent uncertainty during the process of evaluating each mobile node in the routing process. In specific, MDM and BWM are included into the proposed NIRIMDMP to determine comprehensive attribute weights based on the calculated objective and subjective weights that could be possible derived in routing. It extended the merits of classical RIM using Z numbers to confirm reliable ranking of mobile nodes, even when the optimal solution exists amid extreme values taken into consideration for assessing the mobile nodes during decision making. Simulation investigations of the proposed NIRIMDMP confirmed improved throughput and network lifetime with reduced control overhead, energy consumptions, and delay independent of the amount of malicious and non‐cooperative nodes.
Storm‐flood‐dominated delta succession in the Pleistocene Taiwan Strait
Authors: Vaucher Romain , Dillinger Antoine , Hsieh Amy I. , Chi Wen‐Rong , Löwemark Ludvig , Dashtgard Shahin E. 
Published: May 2023

AbstractStorm‐flood‐dominated deltas are sedimentary systems in which a complex interplay of hydrodynamic processes occurs during storms (e.g. tropical cyclones) due to the coeval action of continental and oceanic processes. This paper reports on a superbly exposed, 135.5 m thick stratigraphic succession of the Pleistocene Cholan Formation exposed along the Da'an River, Taiwan. The sedimentary succession comprises alternating mudstone and sandstone, is mostly fine‐grained, and exhibits multiple event beds that record deposition during tropical cyclones and post‐depositional deformation features produced during earthquakes. Detailed facies analyses reveal that deposition towards the base of the succession occurred in the palaeo‐Taiwan Strait in storm‐flood‐dominated prodelta and delta‐front environments passing upwards into delta‐plain environments. Tropical cyclone beds are encountered throughout the subaqueous storm‐flood delta successions, and are identified by (i) trough cross‐stratified sandstone bedsets with erosive bases that contain both mud clasts and mudstone beds, (ii) sandstone with aggrading wave ripples and (iii) hummocky cross‐stratified sandstone with rare gutter casts filled with coal fragments and shell remains. Tropical cyclone deposits are either top‐down burrowed or capped by massive or laminated mudstone. Seismites are rare and are mainly recognised through soft‐sediment deformation of beds; they do not show evidence of slope failure. Compared to storm‐flood delta successions described elsewhere, the Cholan Formation shows significantly fewer oscillatory‐generated sedimentary structures and gutter casts. This difference is attributed to the Cholan Formation being deposited in and along the margin of a strait characterised by strong shore‐parallel currents and relatively small storm waves due to its position between Taiwan and mainland China. This study refines depositional process interpretations of the Cholan Formation, provides criteria for recognising storm‐flood delta deposits in tectonically active straits with multiple sediment sources fed by steep drainages and short river catchments, and provides additional criteria for recognising tropical cyclone deposits in shallow‐marine settings.
Adverse events seen with fillers
Authors: Lateef Almeera , Barker Catherine , Snyder Alan , Vaishnav Yash , Schlesinger Todd 
Published: May 2023

AbstractDermal fillers are often utilized to enhance facial features as well as correct signs of aging on the face. Common side effects seen with dermal fillers are injection site erythema, edema, and secondary infection. Uncommon yet severe effects of filler injection result from vascular occlusion, causing skin necrosis, blindness, or even stroke. Vision loss typically occurs immediately after injection. Partial vision loss is associated with better prognosis. In case of visual compromise, an ocular massage and reduction of ocular pressure should be initiated immediately. The instruments used as well as the pressure and volume of the injection can contribute to unwanted complications. Anti‐thrombolytics, including hyaluronidase for hyaluronic acid injection, may offer some benefit in the case of vascular occlusion. Aesthetic providers must have thorough knowledge of facial anatomy and proper injection technique to mitigate the risk of these dreaded adverse events.
Drought, fire, and rainforest endemics: A case study of two threatened frogs impacted by Australia's “Black Summer”
Authors: Heard Geoffrey W. , Bolitho Liam J. , Newell David , Hines Harry B. , Norman Patrick , Willacy Rosalie J. , Scheele Ben C. 
Published: May 2023

AbstractDeepening droughts and unprecedented wildfires are at the leading edge of climate change. Such events pose an emerging threat to species maladapted to these perturbations, with the potential for steeper declines than may be inferred from the gradual erosion of their climatic niche. This study focused on two species of amphibians—Philoria kundagungan and Philoria richmondensis (Limnodynastidae)—from the Gondwanan rainforests of eastern Australia that were extensively affected by the “Black Summer” megafires of 2019/2020 and the severe drought associated with them. We sought to assess the impact of these perturbations by quantifying the extent of habitat affected by fire, assessing patterns of occurrence and abundance of calling males post‐fire, and comparing post‐fire occurrence and abundance with that observed pre‐fire. Some 30% of potentially suitable habitat for P. kundagungan was fire affected, and 12% for P. richmondensis. Field surveys revealed persistence in some burnt rainforest; however, both species were detected at a higher proportion of unburnt sites. There was a clear negative effect of fire on the probability of site occupancy, abundance and the probability of persistence for P. kundagungan. For P. richmondensis, effects of fire were less evident due to the limited penetration of fire into core habitat; however, occupancy rates and abundance of calling males were depressed during the severe drought that prevailed just prior to the fires, with the reappearance of calling males linked to the degree of rehydration of breeding habitat post‐fire. Our results highlight the possibility that severe negative impacts of climate change for montane rainforest endemics may be felt much sooner than commonly anticipated under a scenario of gradual (decadal‐scale) changes in mean climatic conditions. Instead, the increased rate of severe stochastic events places these narrow range species at a heightened risk of extinction in the near‐term.
Plastic maternal effects of social density on reproduction and fitness in the least killifish, Heterandria formosa
Authors: Levell Samantha T. , Bedgood Samuel A. , Travis Joseph 
Published: May 2023

AbstractEnvironmental parental effects, also known as transgenerational plasticity, are widespread in plants and animals. Less well known is whether those effects contribute to maternal fitness in the same manner in different populations. We carried out a multigenerational laboratory experiment with females drawn from two populations of the least killifish, Heterandria formosa, to assess transgenerational plasticity in reproductive traits in response to differences in social density and its effects on maternal fitness. In the first and second generations, increased density decreased reproductive rate and increased offspring size in females from both populations. There were complicated patterns of transgenerational plasticity on maternal fitness that differed between females from different populations. Females from a population with historically low densities whose mothers experienced lower density had higher fitness than females whose mothers experienced higher density, regardless of their own density. The opposite pattern emerged in females from the population with historically high densities: Females whose mothers experienced higher density had higher fitness than females whose mothers experienced lower density. This transgenerational plasticity is not anticipatory but might be considered adaptive in both populations if providing those “silver spoons” enhances offspring fitness in all environments.
Rift Valley fever virus outbreak in Mauritania yet again in 2022: No room for complacency
Authors: Tabassum Shehroze , Naeem Farhan , Azhar Masood , Naeem Aroma , Oduoye Malik O. , Dave Tirth 
Published: May 2023

AbstractRift Valley fever is an important yet ignored viral hemorrhagic fever claiming many lives of African and Arabian countries over the past decade. Unfortunately, a recent outbreak of Rift Valley fever is currently ravaging in Mauritania. Death toll is rising continuously with 23 deaths reported in the month of October, 2022. Our article aims to shed light on the ongoing Rift Valley fever outbreak and recommendations to eradicate this potential threat to public health. Online databases including PubMed, the Lancet, and Science Direct as well as conferences, news, and press releases were used to for data collection. All the available medical literature related to Rift Valley fever in Mauritania were taken into consideration while writing the manuscript. As of October 17, 2022, 47 cases have been documented out of which 23 are dead. Case fatality rate has been reached to 49% which has given a wakeup call to the authorities. Efforts are being made by the concerned authorities and World Health Organization to halt the progression of this outbreak. Further investigations are required to completely eradicate the recurrent outbreaks in Mauritania especially in the area of vaccine development. Active involvement of public with the government authorities is of extreme significance in combating this disease.
Target selection and clinical chimeric antigen receptor T cell activity against solid tumors
Authors: von Hofe Eric , Yang Yanping , Jin Moonsoo M. 
Published: May 2023

AbstractChimeric antigen receptor (CAR) T cell therapy is a relatively new form of targeted therapy that has demonstrated impressive success in treating hematological malignancies. It has been challenging to translate this success to solid tumors. Reasons for this include barriers to delivery, tumor heterogeneity, cancer cells' ability to evade the immune system as well as identifying the optimal target. Most CAR T clinical trials have targeted well‐characterized cancer targets with significant preclinical and in some cases clinical validation. Published results from some of these trials show signs of anti‐cancer activity that warrant encouragement, but also caution, given instances of unacceptable toxicity. The narrow therapeutic window is complicated by the ability of CAR T cells to expand in patients regardless of dose. Here, we review those trials showing encouraging results in the context of target selection. It is clear that more specific tumor targeting is required, either by affinity tuning to avoid low‐level target expression in healthy cells, logic gating, or the identification of new targets that are more cancer specific.
Natural 3D Extra Cellular Matrix mimicking stem cells seeded decellularized scaffolds as a platform for tendon regeneration
Authors: Niveditha K. , John Annie , Joseph Josna , Mini S. , Vineeth C. A. , Swapna T. S. , Abraham Annie 
Published: May 2023

AbstractAchilles tendon, which connects the calf muscles to heel, is the strongest tendon in the body. Despite its strength, it is more prone to injury due to its limited blood supply. Tendon‐related injuries are more common in sportspersons, people with labor‐intensive work and the aged community. The currently available treatment mode is surgery which is expensive with chances of re‐injury. Present study made an attempt to fabricate a tissue‐engineered tendon product using decellularized tendon (DT) seeded with stem cells and bioactive components of Tinospora cordifolia extract (TCE). The bare DT tissue scaffold/substitute may also serve as a drug delivery platform for growth factors and cells with a new approach to promote tissue regeneration in clinical applications. DT construct showed good regenerative potential and easily promoted new tissue formation. Decellularization of the tendon was carried out by chemical method using tri (n‐butyl) phosphate (TnBP). DT was physicochemically characterized by contact angle measurement, thermal gravimetric analysis (TGA), and mechanical testing. Rabbit adipose derived mesenchymal stem cells (RADMSCs) were isolated and phenotypically characterized by flow cytometry analysis, tri lineage differentiation, and so forth. Further, stem cell seeded DT scaffolds were prepared and found to be non‐toxic by cytotoxicity, cell adhesion by scanning electron microscope (SEM) analysis, cell viability by live dead assays, and so forth. The findings of this study yield valid proof for the employability of cell‐seeded DT construct as a natural scaffold in repairing injured tendons—the toughest chords of the skeleton. This is a cost effective method for the replacement of injured/damaged tendons for athletes, people in labor‐intensive occupations, the elderly population, and so forth—a boon towards the repair of the tendon in damage/injury.
School counselors’ experiences strengthening classroom instruction through professional development
Authors: Dack Hilary , Merlin‐Knoblich Clare 
Published: May 2023

AbstractThe field of school counseling has evolved to feature varied means of supporting students, including individual counseling, group counseling, and classroom instruction. However, classroom instruction is typically overlooked in school counselor training programs, and school counselors often lack the skills needed to design effective lessons. This longitudinal study explored the experiences of 21 school counselors in a year‐long, post‐master's professional development program focused on improving classroom instruction. Data were drawn from interviews, curricular artifacts, and reflective journals and analyzed using interpretive qualitative methodology. This study's contributions lie in findings that illustrate school counselors’ nuanced perceptions of the utility of the Understanding by Design instructional approach as a framework for implementing ASCA Student Standards; the utility of learning science as a framework for designing classroom instruction that leads to long‐term learning; and supports or challenges to implementing classroom instruction in individual contexts.
Post‐hatching developmental changes in the adrenal gland of the Japanese quail (Coturnix coturnix japonica): Histological, immunohistochemical, and electron microscopic studies
Authors: Abdel‐maksoud Fatma M. , Fadl Saher , Abou‐Elmagd Ahmed , Saleh Abdelmohaimen M.M. 
Published: May 2023

AbstractThe adrenal glands are paired abdominal endocrine organs vital to the bird's health. The present research aimed to provide a comprehensive examination of the histological, ultrastructural, and immunohistochemical investigations of the adrenal gland in Japanese quail during the post hatching period. The current study was performed on 21 healthy Japanese quail chicks at different post hatching periods. Our results showed the adrenal gland is surrounded by a connective tissue capsule, which consists of dense collagen fibers containing large blood vessels, chromaffin cells, autonomic ganglia, fibroblasts, and migrating Schwann cells. The zonation of the adrenal gland is composed of a subcapsular layer, a peripheral zone, and a central zone, which gets more pronounced with age. At the ultrastructural level, the interrenal cells take the steroid‐secreting cells characters; they have varying amounts of lipid droplets and abundant mitochondria. Adrenal medullary chromaffin cells showed positive immunoreactivity to the NSE. With increasing age, the chromaffin tissue's Sox10 immunoreactivity increased. β‐catenin is expressed within the plasmalemma and the cytoplasm of the interrenal and chromaffin cells and its reactivity increased with age, especially in the chromaffin cells. Our results indicate the adrenal gland undergoes significant morphological changes during the postnatal life. Overall, the postnatal period is an important time for the development and maturation of the adrenal glands.