bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility
Authors: Rimbawanto Efka Aris , Yusiati Lies Mira , Baliarti Endang , Utomo Ristianto 
Published: March 2023

Two plants as condensed tannin (CT) sources were supplemented to protein trash fish silage (TFS) to observe their effect on in vitro ruminal fermentation product, micobial protein synthesis, and digestibility. CT supplementation on protein trash fish silage was on control proportion (0), under optimum level (2.0%), at optimum level (4.0%), and above optimum level (6.0%) of g TFS); of protein precipitation of bovine serum albumin (BSA) with CT from L. leucocephala; and under optimum level (1.5%), at optimum level (3.0%) and above optimum level (4.5% of g TFS), BSA protein precipitation with CT from C. calothyrsus. The effect on degradation in ruminal fluid and ruminal fluid followed by incubation in HCl-pepsin was evaluated using a modified two-step in vitro method. The CT level of L. leucocephala and C. calothyrsus was 4% and 6%, respectively with protein precipitation BSA was 26.25 and 31.77 g BSA/g CT, respectively. CT supplementation to trash fish silage, ruminal fermentation product (NH3-N, C2, C3, and total VFAs) and digestibility (DM and OM) decreased (P<0.01) and increased (P<0.01) total CP digestibility (in HCl-pepsin). The difference was attributed to CT source without affecting ruminal microbial protein synthesis. CT of L. leucocephala was better in increasing total protein digestibility (70%) than that of C. calothyrsus (15%). It indicated that CT of C. calothyrsus was less effective in protected TSF protein degradation in rumen compared to that of L. leucocephala.
Effect of Supplementation Purslane (Portulaca oleracea) as a Source of Alpha-Linolenic Acid on Production Performance and Physical Quality of Egg of Laying Hens
Authors: Kartikasari Lilik , Nuhriawangsa Adi Magna Patriadi , Hertanto Bayu Setya , Swastike Winny 
Published: March 2023

The aim of the study was to evaluate the effects of inclusion plant source of n 3 fat in the form of alpha-linolenic acid (ALA, 18:3n-3) on the diets of layers on production performance and physical quality of eggs. A total of 125 Hy-Line Brown hens (38 weeks old) were placed at individual cages and assigned to five dietary treatments. The dietary treatments were supplemented with 0, 1.5, 3.0, 4.5 and 6.0% purslane meal. Laying hens were fed for five weeks following a seven day adaptation period. Water and feed were provided ad libitum. Feed intake (FI) measured weekly and feed consumption ratio (FCR) was calculated at the end of the trial. A total of 25 egg yolk samples of day 35 (n = 5 egg yolks for each treatment) were collected to analyse physical quality of eggs. The data were analyzed using Analysis of Variance (ANOVA). Differences between treatment means were further analyzed using Duncan's New Multiple Range Test (DMRT). Results showed that the incorporation of plants rich in ALA did not modify FI, FCR, and egg production. Supplementation of purslane meal in the diets had no effect on physical quality of eggs, including egg weight, yolk weight, albumen index, yolk index and Haugh Unit (HU). The average of egg weight and yolk weight were 60,5 and 15.3 g, respectively. Diet containing purslane meal increased yolk colour. In conclusion, laying hens that fed diet supplemented with purslane meal rich in ALA improved yolk colour and did not change the production performance of the laying hens or the qualities of the eggs.
Nutritional Status Evaluation (Estimated Using Lignin Internal Indicator) of Grazed Yearling Kacang Bucks
Authors: Adiwiniarti Retno , Budisatria I Gede Suparta , Kustantinah Kustantinah , Rusman Rusman , Purnomoadi Agung 
Published: March 2023

In some areas in Indonesia, farmers usually graze their goats during the day and house them at night. The goats eat grass without additional concentrate. However, how much nutrient intakes of grazing goats in Indonesia are seldom calculated. Therefore, this research was set up to investigate the productivity and to estimate the dry matter, protein, and TDN intake of grazed Kacang buck using lignin internal indicator. About 12 heads of Kacang buck, aged 1-1.5 year (the incisors have erupted 1), having the initial body weight of 18.42+1.78 kg (CV= 9.67%) and reared by farmers in Grobogan, Central Java, Indonesia were used in this study. Intake and nutrient digestibility of the buck were calculated using lignin as internal marker. After being reared 86 days, the average daily gains (ADG) of the buck were 3.20+21.14 gram ( -26.16 gram to 53.60 gram). Low ADG of the buck was caused by low DMI, protein and TDN intake. The Kacang bucks were usually grazed in the soccer fields, back yards, and roadsides, therefore their intake were lower than their maintenance needed. The averages of DMI, protein and TDN intake were about 1.17+0.17 % their body weight, 30.16+4.18 g and 91.45+11.64 g, respectively. It can be concluded that the productivity of grazing Kacang bucks is relatively very low.
Performance Indices and Physiological Changes in Pearl Guinea Fowls (Numida Meleagris) Supplemented with Molasses through Drinking Water
Authors: Habibu Buhari , Ikira Nizam Mustapha , Ibrahim Moses , Buhari Hajarah Uwale , Abdullahi Abdullahi , Ibrahim Muhammed Abdul , Kawu Mohammed Umar 
Published: March 2023

Abstract. The study was aimed at evaluating the performance indices and physiologic changes in pearl guinea fowls (Numida meleagris) supplemented with molasses through drinking water. A total of thirty-two (n=32) day-old pearl guinea fowls raised to 12-week-old were used for the study. The fowls were randomly allocated to two groups (control and experimental) of 16 fowls each, with each having two replicates. Experimental fowls were given 5 mL molasses per litre of drinking water for 8 weeks, while control fowls were given only drinking water. Performance indices which include: feed consumption, water intake and percentage weight gain were measured. Rectal temperature was measured and blood samples were collected to evaluate the changes in haematological parameters, serum triiodothyronine (T3) and thyroxine (T4) concentrations. Results revealed that the molasses-treated fowls had significantly (P<0.05) higher percentage weight gain, blood total protein, packed cell volume and mean corpuscular volume compared to control. However, the feed consumptionwas significantly lower (P<0.05) in the molasses-treated fowls compared to control. In both groups, rectal temperature increased significantly (P<0.05) during the afternoon hours and was lower (P<0.05) in molasses-treated fowls than control during the evening hours. There was no significant difference in other haematological parameters, serum glucose, T4 and T3 betweengroups. In conclusion, molasses supplementation decreased feed consumption, improved body weight gain and enhanced nutritional status and erythropoiesis in guinea fowls.
Human and machine learning pipelines for responsible clinical prediction using high-dimensional data
Authors: Sufriyana Herdiantri , Wu Yu Wei , Su Emily Chia-Yu 
Published: March 2023

Abstract
This protocol aims to develop, validate, and deploy a prediction model using high dimensional data by both human and machine learning. The applicability is intended for clinical prediction in healthcare providers, including but not limited to those using medical histories from electronic health records. This protocol applies diverse approaches to improve both predictive performance and interpretability while maintaining the generalizability of model evaluation. However, some steps require expensive computational capacity; otherwise, these will take longer time. The key stages consist of designs of data collection and analysis, feature discovery and quality control, and model development, validation, and deployment.
Inclusion of macromolecular crowders in three-dimensional human gingival fibroblast cultures
Authors: Ramalingam Rajesvaran , Häkkinen Lari 
Published: March 2023

Abstract
Fibroblast-derived extracellular matrix (ECM) niche regulates cell phenotype and behavior relevant to development, tissue homeostasis, wound healing, and pathological conditions. Improved understanding of cell-ECM interactions requires culture models that mimic the extracellular microenvironment found in vivo. Unlike in vivo, cultured cells reside in aqueous media containing a low concentration of macromolecules. Inclusion of inert macromolecular crowders (MMCs) allows to better mimic in vivo-like conditions in vitro in a phenomenon known as macromolecular crowding (MMC). We present here a standardized protocol to utilize MMCs (Ficoll 70/400) to generate human gingival fibroblast cultures that can be propagated at least for 21 days. The resulting cultures are composed of cells embedded in their own three-dimensional ECM and display gene expression and ECM composition that are distinct from that observed under non-crowded conditions.
Standard Operating Protocol: Implantation of Cambrige NeuroTech chronic silicon probe and mini-amp-64 digital headstage in mice
Authors: Jacobs Thomas , Darch Henry , Holtzman Tahl , de Zeeuw Chris , Romano Vincenzo 
Published: March 2023

Abstract
In this protocol, all steps required to perform implantation of a Cambridge NeuroTech chronic silicon probe and mini-amp-64 digital headstage, for cerebellar single-unit electrophysiology (above Crus I/II) are described, alongside detailed pictures of the procedure. This protocol starts by describing the steps needed to assemble the probe and nano-Drive; required to adjust probe position in the brain once implanted. The protocol then proceeds into the surgical procedure itself, which highlights all the important steps to perform successful implantation by means of constructing a small, lightweight and user-configurable headpiece implant.
Enhancement of Sapindus Trifoliatus Biodiesel Production and Performance using Mg-doped CaO Nanocatalyst
Authors: Muninathan Kota , Ramanan Madhavan Venkata 
Published: March 2023

Abstract

The use of biodiesel can reduce dependence on diesel and stabilize the economy when using internal combustion engines. CI engine's performance with biodiesel is substantially low but could be improved by emulsifying nano-additives with biodiesel. Magnesium-doped Calcium Oxide, a novel nanocatalyst is emulsified in its nano form with the Sapindus Trifoliatus biodiesel (STBD) at B25 blend (STBD25+Mg-CaO 30ppm) and tested in the CI engine. The performance results obtained are compared to the baseline values ​​obtained using diesel, Sapindus Trifoliatus biodiesel (STBD25), and Sapindus Trifoliatus biodiesel blended with Cao nano additives (STBD25+30ppm CaO) in the CI engine. . A maximum biodiesel yield of 91.75% was achieved at 58°C, 73 min, a catalyst concentration of 4% (%w / %w) and a methanol: oil molar ratio of 15:1. From the comparison, it is revealed that (i) In-cylinder pressure found to be increased by 3.22%, 6.24% and 9.02%, (ii) HRR increases by 1.68%, 16.69% and 32.5%, (iii) BTE is found to be increased by 4.56%, 8.23% and 11.79%, (iv) UHC decreases by 21.12%, 6.06% and 11.43%, (v) CO decreases by 0.04%, 0.01% and 0.01%, (vi) Smoke decreases by 54.52%, 9.34% and 19.58%, compared with diesel, STBD25+30ppm CaO and STBD25. NOx released while using STBD25+Mg doped CaO in CI engine was observed to decrease compared with STBD25 and STBD25+CaO (by 10.72 % and 18.64 %) and increase compared with diesel (by 8.64%). Whereas an insignificant drop in NOx was observed while using STBD25+Mg doped CaO as fuel compared with STBD25+CaO and STBD25 in spite of a significant increase in HRR and BTE which might be due to the capture of excess oxygen by Mg during the combustion diffusion phase.
Temperature Analysis of Thermosiphon Solar Water Heaters Using Different Flat Plate Collectors and MATLAB software.
Authors: Mahani Norul Ahsanah Aulia Mohamad , Awang Mariah , Hamidon Nuramidah , Rahman Mohd Shahril Abdul , Yusop Fatimah Mohamed , Rahman Mohammad Ashraf Abdul , Musa Mohd Kamaruzaman , Ahmad Faridahanim , Daud Siti Zaleha 
Published: March 2023

Abstract
Solar thermal energy was utilized to heat water for its useful applications. Moreover, solar water heaters have become essential in our lives, and using renewable energy sources is becoming more popular. The current effort is to test and find the effectual solar water heating system. Solar water heater designs have been suggested and produced within two methods whereas forced circulation using a mechanical pump and natural circulation without the use of a pump also recognized as thermosiphon solar water heater. This research was to analyse most efficient Flat Plate Collector (FPC) in terms of heat absorption on Thermosiphon Effect due to energy collection by different colour, material, and glazing. This experiment uses Solar Energy Trainer and MATLAB software to compare the impact of several forms FPC on heat absorption of thermosiphon effect in solar water heater. The most efficient FPC in terms of colour is black collector with higher efficiency of 9.3% compared to white while most efficient FPC in terms of material and glazing are copper and double-glazing collector with 10.86% and 11.15% respectively. The experiment and analysis show that various types of FPC with good heat absorber have higher efficiency.
Factors predicting the intention of drug abuse avoidance among adolescents in Pinlaung Township, Myanmar: Predictive correlational design
Authors: Latt Nang Nwe Nwe , Putdivarnichapong Wimolnun , Phetrasuwan Supapak , Vongsirimas Nopporn 
Published: March 2023

Abstract

Background:Adolescence is a critical transitional period when young adults are at high risk for taking and abusing drugs and controlled substances. In Myanmar, drug use is common among adolescents and is a public health concern. Drug abuse prevention education has not been studied or evaluated among the Myanmar adolescent population. This study investigated the effects of sex, parental history without drug/alcohol abuse, self-efficacy, parental marital status, and family functioning on the intention of drug abuse avoidance among Myanmar adolescents.
Methods:This is a predictive correlational study. The Biopsychosocial Model of Engle (1977) provided the theoretical framework for this study. A purposive sampling method was employed to collect data from 157 students between 13-18 years old in middle and high school in Palaung Township, Southern Shan State, Myanmar, during the Covid-19 pandemic and political movement. The sample size was calculated using G* power software. Four self-administered questionnaires: a socio-demographic questionnaire, the Thai Family Functioning Scale (TFFS), the General Self-Efficacy Scale (GSE), and the Intention of Drug Avoidance Scale (IDAS) were used to collect the data. Multiple linear regression was employed to analyze the data.
Results: Five predictors, including sex, parental history without drug/alcohol abuse, self-efficacy, parental marital status, and family functioning, explained 24.4% of the variance in the intention of drug abuse avoidance among Myanmar adolescents. In addition, only three factors, sex (β= -.25, p<.01), self-efficacy (β= .16, p<.05), and family functioning (β= .31, p<.001) statistically and significantly predicted the intention of drug abuse avoidance among Myanmar adolescents.
Conclusions: Self-efficacy, female gender, and family functioning predicted the intention of drug abuse avoidance among Myanmar adolescents in Palaung Township, Southern Shan State, Myanmar.
Implications of this study: The results of this study have implications for all stakeholders involved in drug abuse prevention through research, education, practice, and policymaking of psychoeducational programs, developing interventions for substance abuse prevention, and issuing policies leading to improvements in intentions of drug abuse avoidance among Myanmar adolescents.
Genome-wide association study of lipids in Greenlandic Inuit shows independent PCSK9 association and reveals a unique genetic architecture
Authors: Senftleber Ninna , Andersen Mette , Jørsboe Emil , Stæger Frederik , Nøhr Anne , Garcia-Erill Genis , Meisner Jonas , Santander Cindy , Balboa Renzo , Gilly Arthur , Bjerregaard Peter , Larsen Christina , Grarup Niels , Jørgensen Marit , Zeggini Eleftheria , Moltke Ida , Hansen Torben , Albrechtsen Anders 
Published: March 2023

Abstract
Perturbation of lipid homeostasis is a major risk factor for cardiovascular disease (CVD), the leading cause of death worldwide. We aimed to identify genetic variants affecting lipid levels, and thereby risk of CVD, in Greenlanders. Genome-wide association studies (GWAS) of six blood lipids, triglycerides, LDL-cholesterol, HDL-cholesterol, total cholesterol, as well as apolipoproteins A1 and B, were performed in up to 4473 Greenlanders. For genome-wide significant variants, we also tested for associations with additional traits, including CVD events. We identified 11 genome-wide significant loci associated with at least one of the lipid traits, and a potential causal variant near PCSK9 (rs12117661), which was independent of the known PCSK9 loss-of-function variant (rs11491147). rs12117661 was associated with lower LDL-cholesterol (βSD(SE)=-0.22 (0.03), p=6.5x10-12) and total cholesterol (-0.17 (0.03), p=1.1x10-8) in the Greenlandic study population. Similar associations were observed in Europeans from the UK Biobank, where the variant was also associated with a lower risk of CVD outcomes. Moreover, rs12117661 was a top eQTL for PCSK9 across tissues in GTEx, and was located in a predicted regulatory element, supporting a possible causal impact on PCSK9 expression. Combined, the 11 GWAS hits explained up to 16.3% of the variance of the lipid traits. This suggests that the genetic architecture of lipid levels in Greenlanders is markedly different from Europeans, with fewer variants explaining the variance.
Diabetes Management through Telementoring of Primary Care Providers and Community Health Workers: Changes in Patient-Reported Outcomes
Authors: Paul Margaret M. , Saad Andrea Davila , Billings John , Blecker Saul , Bouchonville Matthew F. , Chavez Cindy , Arora Sanjeev , Berry Carolyn A. 
Published: March 2023

Abstract
Background
Endocrine ECHO (Extension for Community Healthcare Outcomes) is a scalable model of healthcare education that extends enhanced training through ongoing telementoring of community primary care providers (PCPs) by a team of specialists. The purpose of this study was to assess whether patients of providers engaged in the Endocrine ECHO program (Endo ECHO) exhibited improvements in lifestyle, and self-management behaviors.
Methods
A sample of 533 adult Endo ECHO patients diagnosed with type 1 or complex type 2 diabetes (insulin dependent and/or with an HbA1c of 9% or higher) took a comprehensive survey during Endo ECHO study enrollment (pre-test) and again after at least one year past their first exposure to the intervention (post-test). We assessed pre/post changes in patient-reported outcomes on well-being, lifestyle, and self-management behaviors using McNemar’s tests for paired data.
Results
A broad array of patients’ self-reported well-being, lifestyle, and self-management behaviors improved over the course of their engagement in the Endo ECHO program. Despite relatively low average health literacy and numeracy, patients’ confidence and self-efficacy to manage their disease also improved significantly from pre-test to post-test.
Discussion
Endo ECHO may be an effective means of enhancing primary care for individuals with type 1 and complex type 2 diabetes and improving patient capacity to perform the extensive array of lifestyle and self-care behaviors necessary to effectively manage their diabetes outside of the clinical setting.
Conclusions
The ECHO model of care has been shown to improve provider and satisfaction, quality of care, and in some cases clinical outcomes across various program areas. This study indicates that endocrinology-focused ECHO programs may also lead to behavior changes among patients in between visits to their provider. Further research should examine the impact of the ECHO model on patient behavior.
SCF and IL-33 regulate mouse mast cell phenotypic and functional plasticity supporting a pro-inflammatory microenvironment
Authors: Lecce Mario , Milito Nadia , Putro Erisa , Pietropaolo Giuseppe , Marangio Caterina , Scarno Gianluca , Moretti Marta , De Smaele Enrico , Santini Tiziana , Bernardini Giovanni , Sciumè Giuseppe , Santoni Angela , Molfetta Rosa , Paolini Rossella 
Published: March 2023

Abstract
Mast cells (MCs) are multifaceted innate immune cells often present in the tumor microenvironment (TME). Several recent findings support their contribution in the transition from chronic inflammation to cancer. However, MC-derived mediators can either favor tumor progression, inducing spread of the tumor, or exert anti-tumorigenic functions, limiting tumor growth.
This apparent controversial role likely depends on the plastic nature of MCs that under different microenvironmental stimuli can rapidly change their phenotype and functions.
Thus, the exact effect of unique MC subset(s) during tumor progression is far from being understood. Using a murine model of colitis-associated colorectal cancer, we initially characterized MC population within the TME and in non-lesional colonic areas, by multicolor flow cytometry and confocal microscopy. Our results demonstrated that tumor-associated MCs harbor a main connective tissue phenotype and release high amounts of Interleukin (IL)-6 and Tumor Necrosis Factor (TNF)a. This MC phenotype correlates with the presence of high levels of Stem Cell Factor (SCF) and IL33 inside the tumor.
Thus, we investigated the effect of SCF and IL-33 on primary MC cultures and underscore their ability to shape MC phenotype eliciting the production of pro-inflammatory cytokines.
Our findings support the conclusion that during colonic transformation a sustained stimulation by SCF and IL-33 promotes the accumulation of a prevalent connective tissue-like MC subset that through the secretion of IL-6 and TNF-a maintains a pro-inflammatory microenvironment.
Health Literacy predicts incident foot ulcers after 4 years – the SHELLED cohort study
Authors: Chen Pamela , Callisaya Michele , Wills Karen , Greenaway Timothy , Winzenberg Tania 
Published: March 2023

Abstract
Aims/hypothesis: To determine whether health literacy is associated with an index diabetes-related foot ulcer (DFU).
Methods: The SHELLED Study is a 4-year prospective study of people with diabetes aged over 40 with no history of DFU. The primary outcome was development of a first foot ulcer. Health Literacy was measured using the short form Test of Functional Health Literacy in Adults (s-TOFHLA) and nine domains of the Health Literacy Questionnaire (HLQ).
Results: Of 222 participants, 191 (86.0%) completed the study, of whom 13 (5.9%) developed an incident ulcer. In multivariable models, every unit increase in S-TOFHLA score reduced the odds of foot ulcer development by 6% (OR 0.94, 95% CI 0.88 to 0.99). Better scores on two HLQ domains reduced the odds of foot ulcer (actively managing my health (OR 0.23, 95% CI 0.08 to 0.65) and understanding health information well enough to know what to do (OR 0.39, 95% CI 0.19 to 0.78). This was independent of baseline risk for foot disease.
Conclusions/interpretation: These data provide novel evidence that health literacy is an important clinical risk factor for index foot ulceration. People with poor health literacy should be identified for tailored educational programs for DFU prevention.