bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metacognition in the audiovisual McGurk illusion: perceptual and causal confidence
Authors: Meijer David , Noppeney Uta 
Published: March 2023

SummaryAlmost all decisions in everyday life rely on multiple sensory inputs that can come from common or independent causes. These situations invoke perceptual uncertainty about environmental properties and the signals’ causal structure.Using the audiovisual McGurk illusion this study investigated how observers formed perceptual and causal confidence judgments in multisensory integration tasks under causal uncertainty. Observers were presented with spoken syllables, their corresponding articulatory lip movements, or their congruent and McGurk combinations (e.g. auditory ‘B/P’ with visual ‘G/K’). Observers reported their perceived auditory syllable, the causal structure and confidence for each judgement.Observers were more accurate and confident on congruent than unisensory trials. Their perceptual and causal confidence were tightly related over trials as predicted by the interactive nature of perceptual and causal inference. Further, observers assigned comparable perceptual and causal confidence to veridical ‘G/K’ percepts on audiovisual congruent trials and their causal and perceptual metamers on McGurk trials (i.e. illusory ‘G/K’ percepts). Thus, observers metacognitively evaluate the integrated audiovisual percept with limited access to the conflicting unisensory stimulus components on McGurk trials.Collectively, our results suggest that observers form meaningful perceptual and causal confidence judgments about multisensory scenes that are qualitatively consistent with principles of Bayesian causal inference.
Nanopore adaptive sampling of a metagenomic sample derived from a human monkeypox case
Authors: Hewel C , Schmidt H , Runkel S , Kohnen W , Schweiger-Seemann S , Michel A , Bikar S-E , Plachter B , Hankeln T , Linke M , Gerber S 
Published: March 2023

AbstractIn 2022, a series of human monkeypox cases in multiple countries led to the largest and most widespread outbreak outside the known endemic areas. Genomic surveillance is of utmost importance to control such outbreaks. To this end, we performed Nanopore Whole Genome Sequencing of a local monkeypox sample on a PromethION 24. Adaptive sampling was applied forin silicodepletion of the human host genome, allowing for the enrichment of low abundance viral DNA withouta prioriknowledge of sample composition. Nanopore sequencing allowed for high viral genome coverage, tracking of sample composition during sequencing, strain determination, and preliminary assessment of mutational pattern. Nanopore sequencing is a highly versatile method to characterize a virus in real-time without pre-sequencing target enrichment.
Mechanistic and structural insights into a divergent PLP-dependent L-enduracididine cyclase from a toxic cyanobacterium
Authors: Cordoza Jennifer L. , Chen Percival Yang-Ting , Blaustein Linnea R. , Lima Stella T. , Fiore Marli F. , Chekan Jonathan R. , Moore Bradley S. , McKinnie Shaun M. K. 
Published: March 2023

ABSTRACTCyclic arginine noncanonical amino acids (ncAAs) are found in several actinobacterial peptide natural products with therapeutically useful antibacterial properties. The preparation of ncAAs like enduracididine and capreomycidine currently takes multiple biosynthetic or chemosynthetic steps, thus limiting the commercial availability and applicability of these cyclic guanidine-containing amino acids. We recently discovered and characterized the biosynthetic pathway of guanitoxin, a potent freshwater cya-nobacterial neurotoxin, that contains an arginine-derived cyclic guanidine phosphate within its highly polar structure. The ncAA L-enduracididine is an early intermediate in guanitoxin biosynthesis and is produced by GntC, a unique pyridoxal-5’-phosphate (PLP)-dependent enzyme. GntC catalyzes a cyclodehydration from a stereoselectively γ-hydroxylated L-arginine precursor via a reaction that functionally and mechanistically diverges from previously established actinobacterial cyclic arginine ncAA pathways. Herein, we interrogate L-enduracididine biosynthesis from the cyanobacteriumSphaerospermopsis torques-reginaeITEP-024 using spectroscopic, stable isotope labeling techniques, and X-ray crystal structure-guided site-directed mutagenesis. GntC initially facilitates the reversible deprotonations of the α- and β-positions of its substrate prior to catalyzing an irreversible diastereoselective dehydration and subsequent intramolecular cyclization. The comparison ofholo-and substrate bound GntC structures and activity assays on sitespecific mutants further identified amino acid residues that contribute to the overall catalytic mechanism. These interdisciplinary efforts at structurally and functionally characterizing GntC enables an improved understanding of how Nature divergently produces cyclic arginine ncAAs and generates additional tools for their biocatalytic production and downstream biological applications.
The association of the RSC remodeler complex with chromatin depends on prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage inSaccharomyces cerevisiae
Authors: Cuevas-Bermúdez A. , Martínez-Fernández V. , Garrido-Godino A.I. , Jordán-Pla A. , Peñate X. , Martín-Expósito M. , Gutiérrez G. , Govind C.K. , Chávez S. , Pelechano V. , Navarro F. 
Published: March 2023

ABSTRACTThe tripartite interaction between the chromatin remodeler complex RSC, RNA polymerase subunit Rpb5 and prefoldin-like Bud27 is necessary for proper RNA pol II elongation. Indeed lack of Bud27 alters this association and affects transcription elongation. This work investigates the consequences of lack of Bud27 on the chromatin association of RSC and RNA pol II, and on nucleosome positioning. Our results demonstrate that RSC binds chromatin to gene bodies and lack of Bud27 alters this association, mainly around polyA sites. This alteration impacts chromatin organization and leads to the accumulation of RNA pol II molecules around polyA sites, likely to be arrested or slower. Our data suggest that RSC is necessary to maintain chromatin organization around those sites, and any alteration of this organization results in the widespread use of alternative polyA sites. Finally, we also find a similar molecular phenotype that occurs upon TOR inhibition with rapamycin, which suggests that alternative polyadenylation observed upon TOR inhibition is likely Bud27-dependent.
Broad sialic acid usage amongst species D human adenovirus
Authors: Mundy Rosie M. , Baker Alexander T. , Bates Emily A. , Cunliffe Tabitha G. , Teijeira-Crespo Alicia , Moses Elise , Rizkallah Pierre J. , Parker Alan L. 
Published: March 2023

AbstractHuman adenoviruses (HAdV) are widespread pathogens causing infections of the respiratory and gastrointestinal tracts, genitourinary system and the eye. Species D (HAdV-D) are the most diverse species and cause both gastrointestinal tract infections and epidemic keratoconjunctivitis (EKC). Despite being significant pathogens, HAdV-D are understudied and knowledge around basic mechanisms of cell infection is lacking. Sialic acid (SA) usage has been proposed as a major mechanism of cell infection for EKC causing HAdV-D. Here, we provide apo state crystal structures for fiber knob proteins of 7 previously undetermined HAdV-D, and provide crystal structures of HAdV-D25, HAdV-D29 and HAdV-D53 knob proteins bound to SA. Biologically, we demonstrate that removal of cell surface SA reduced infectivity of HAdV-C5 vectors pseudotyped with HAdV-D fiber knob proteins, whilst engagement of the classical HAdV receptor, CAR was variable. Together, these data indicate an important role for SA engagement in the tropism of many HAdV-D and may facilitate the development of suitable antivirals to control EKC outbreaks.
Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells
Authors: Tsuchida Connor A. , Brandes Nadav , Bueno Raymund , Trinidad Marena , Mazumder Thomas , Yu Bingfei , Hwang Byungjin , Chang Christopher , Liu Jamin , Sun Yang , Hopkins Caitlin R. , Parker Kevin R. , Qi Yanyan , Satpathy Ansuman T. , Stadtmauer Edward A. , Cate Jamie H.D. , Eyquem Justin , Fraietta Joseph A. , June Carl H. , Chang Howard Y. , Ye Chun Jimmie , Doudna Jennifer A. 
Published: March 2023

SummaryCRISPR-Cas9 genome editing has enabled advanced T cell therapies, but occasional loss of the targeted chromosome remains a safety concern. To investigate whether Cas9-induced chromosome loss is a universal phenomenon and evaluate its clinical significance, we conducted a systematic analysis in primary human T cells. Arrayed and pooled CRISPR screens revealed that chromosome loss was generalizable across the genome and resulted in partial and entire loss of the chromosome, including in pre-clinical chimeric antigen receptor T cells. T cells with chromosome loss persisted for weeks in culture, implying the potential to interfere with clinical use. A modified cell manufacturing process, employed in our first-in-human clinical trial of Cas9-engineered T cells,1dramatically reduced chromosome loss while largely preserving genome editing efficacy. Expression of p53 correlated with protection from chromosome loss observed in this protocol, suggesting both a mechanism and strategy for T cell engineering that mitigates this genotoxicity in the clinic.
An innovative material for space-oriented structures and habitat design
Authors: Frulla Giacomo , Cestino Enrico , Cumino Federico , Piccolo Alessio , Giulietti Nicola , Fossat Eugenio , Kharrazi Ehsan 
Published: March 2023

Purpose
The purpose of this study is to investigate a new and innovative sandwich material evaluating its capability for use in space habitat structural components in deployable and foldable configurations. The main habitat requirements were considered in the preliminary design of a typical space outpost, proposing a preliminary architecture.

Design/methodology/approach
The stiffness properties of the innovative sandwich (MAdFlex ®) were evaluated using numerical and experimental investigations. Four-point bending tests were performed for complete sandwich characterization. Numerical FE simulations were performed using typical material properties and performance. The application to a space habitat main structure as a basic material has also been discussed and presented.

Findings
MAdFlex basic stiffness performances have been determined considering its double behavior: sufficiently stiff if loaded in a specific direction, flexible if loaded in the opposite direction and enhanced folding performance. Successful application to a typical space habitat confirms the validity and convenience of such a material in designing alternative structures.

Research limitations/implications
The innovative material demonstrates wide potential for structural application and design in demanding space situations under operating conditions and in stored ones at launch.

Practical implications
Several simple deployable structural components can be designed and optimized both for the space environment and for the more traditional terrestrial applications.

Social implications
Simplification in structural design can be derived from deployable low-weight items.

Originality/value
Innovative customized material in sandwich configuration has been proposed and investigated with the aim to demonstrate its potentiality and validity in alternative design architecture.
Comments on the article “Marketing or methodology? Exposing the fallacies of PLS with simple demonstrations” and PLS-SEM in general
Authors: Yuan Ke-Hai 
Published: March 2023

Purpose
The purpose of this paper is to discuss the pros and cons of partial least squares approach to structural equation modeling (PLS-SEM). The topics include bias, consistency, maximization of R2, reliability and model validation.

Design/methodology/approach
The approach in this study is descriptive, and the method consists of logical arguments and analysis that are supported by results in references.

Findings
Several optimal properties of the PLS-SEM methodology are clarified. A proposal for transforming PLS-SEM mode A to mode B is highlighted, and the transformed mode possesses the desired properties of both modes A and B. Issues with the application of regression analysis using composite scores are also discussed. The strength of PLS-SEM is also compared against that of covariance-based SEM.

Research limitations/implications
Additional studies on PLS-SEM are needed when the population structure contains cross-loadings and/or correlated errors.

Practical implications
PLS-SEM may have inflated type I errors and R2 values even with normally distributed data.

Originality/value
The content of this paper is new, and there does not exist such an in-depth discussion of the pros and cons of PLS-SEM methodology in the literature.
Smart tourism city governance: exploring the impact on stakeholder networks
Authors: Ivars-Baidal Josep , Casado-Díaz Ana B. , Navarro-Ruiz Sandra , Fuster-Uguet Marc 
Published: March 2023

Purpose
Building on new trends in tourism and smart city governance, this study aims to examine the degree of interrelation between stakeholder networks involved in tourism governance and smart city development. A model describing the transition towards smart tourism city governance is proposed.

Design/methodology/approach
The proposed model is tested through a multiple case study of seven European cities. This choice of sample makes the study highly representative. Data collection is based on an exhaustive search and analysis of available data on smart city initiatives, destination management organisations and tourism plans. Social network analysis using Gephi software is used to build stakeholder networks.

Findings
Analysis of the stakeholder networks that shape tourism governance and smart initiatives in several cities reveals a disconnection between the two types of networks. The results show limited progress towards the expected synergies of true smart tourism city governance.

Practical implications
Theoretically, the study contributes to the debate on new forms of governance for the complex evolution of urban tourism. In practice, the relationship between tourism governance and smart city initiatives needs to be redefined to achieve synergies that increase the inclusiveness and efficiency of urban tourism policies.

Originality/value
This study examines the under-researched topic of the interrelation between tourism governance and smart city initiatives. By comparing the networks of actors resulting from these two processes, it assesses the extent to which this interrelation helps the emergence of new governance models (smart tourism city governance).
How the real estate indexes have performed during the COVID-19 crisis? Multifractal analysis revisited with wavelet
Authors: Zaouga Ons , Loukil Nadia 
Published: March 2023

PurposeThe purpose of this paper is to test the existence of stylized facts, such as the volatility clustering, heavy tails seen on financial series, long-term dependence and multifractality on the returns of four real estate indexes using different types of indexes: conventional and Islamic by comparing pre and during COVID-19 pandemic.Design/methodology/approachFirstly, the authors examined the characteristics of the indexes. Secondly, the authors estimated the parameters of the stable distribution. Then, the long memory is detected via the estimation of the Hurst exponents. Afterwards, the authors determine the graphs of the multifractal detrended fluctuation analysis (MF-DFA). Finally, the authors apply the WTMM method.FindingsThe results suggest that the real estate indexes are far from being efficient and that the lowest level of multifractality was observed for Islamic indexes.Research limitations/implicationsThe inefficiency behavior of real estate indexes gives us an idea about the prediction of the behavior of future returns in these markets on the basis of past informations. Similarly, market participants would do well to reassess their investment and risk management framework to mitigate new and somewhat higher levels of risk of their exposures during the turbulent period.Originality/valueTo the authors’ knowledge, this is the first real estate market study employing STL decomposition before applying the MF-DFA in the context of the COVID-19 crisis. Likewise, the study is the first investigation that focuses on these four indexes.
Disrupting the fashion retail journey: social media and GenZ's fashion consumption
Authors: Siregar Yuri , Kent Anthony , Peirson-Smith Anne , Guan Congying 
Published: March 2023

PurposeThe aim of this paper is to assess the use of social media by Gen Z consumers and the ways they impact on and re-shape their fashion consumption journey. This generational approach uses the lens of uses and gratifications theory (UGT) to explore the customer fashion retail journey from the perspective of the Gen Z consumer.Design/methodology/approachThe research uses an exploratory approach in response to the relative lack of research in to GenZ consumers combined with a need to understand shopping journeys. Mixed methods were used with a first phase of interviews followed by a survey of 102 Gen Z students recruited online in the UK during the COVID-19 pandemic.FindingsThe study found that GenZ users of social media for shopping sought gratification from experiences derived from social relationships, entertainment and information. The need for immediate gratification was found in new information and meeting new people to maintain social relationships, learn about products and inform the shopping journey. Further, the research supported the importance of visual images in the affective gratification of shopping needs. Resale sites on social media were favoured for their low prices, information about previously owned fashion items and the opportunity to exercise sustainable fashion choices.Originality/valueThe research advances understanding of fashion shopping journeys through social media and online resale sites. It demonstrates that younger consumers, GenZ, shop through the gratification of experiences informed by their social networks and wider contacts. The linear stages of pre to post–purchase shopping are merged and looped as they exchange information about their shopping journey, from information gathering to post–purchase comments. The role of the brand to these knowledgeable consumers conducting their own resale trade is to facilitate access to and information about their products.
Heat resistance and sorptivity of an air-entrained concrete containing mineral admixtures and CBA
Authors: Singh Sandeep , Sharma Shashi Kant , Akbar M. Abdul 
Published: March 2023

Purpose
The purpose of this work is to improve the air entrainment capacity of a concrete by using fine mineral admixtures such as fly ash (FA) and silica fume (SF) as cement substitute, and coal bottom ash (CBA) as fine aggregate substitute. Air entrainment capacity has been studied indirectly as a measure of heat resistance of concrete. Literature has suggested that mineral admixtures improve the air absorption in the paste component of the concrete, on the one hand, whereas they perform pore and grain size refinement, on the other, thereby reducing the air entrainment. CBA, which being porous, creates the possibility of air adsorption by the aggregate component. Therefore, the study finds out whether a double benefit of adding both of these materials will be achieved, or CBA will try to improve the deficiency in the air entrainment created by the mineral admixtures.

Design/methodology/approach
Air-entrained concrete (AEC) mixes were constituted in three groups. First group represents mixes with natural fine aggregates only, and second with 25% fine aggregates substituted by CBA. Progressively, the third group has 50% fine aggregates substituted with CBA. In all the three groups, cement was substituted with FA and SF @ 0%, 20% and 40%, and 0%, 5% and 10%, respectively, thereby creating four binary and four ternary mixes corresponding to each group. Compressive and flexural strength tests were conducted at 28 days on the concrete mixes pre and post high-temperature heat treatment, i.e. 100°C, 200°C and 400°C, respectively. This study also examines the microstructure characteristics of AEC after 14 days of curing via X-ray diffraction. Sorptivity test was also conducted to estimate the capillary and air-entrained voids in concrete.

Findings
It was found that a concrete mix containing 20% FA and 10% SF along with 50% CBA could give similar post-heated strength to a normal (without mineral admixtures) AEC. In AECs where only CBA is present and cement paste is not substituted, both of the pre- and post-heated strengths of concrete reduce. Also, some mixtures containing large amounts of mineral admixtures in concrete with nil CBA show a high reduction in post-heated strength though they show good pre-heated strength. Therefore, mineral admixtures and CBA complement each other in improving the post-heated strength. Air pore structure found from sorptivity test also verifies these results.

Originality/value
AEC is very helpful for insulation of buildings during summer season by absorbing heat waves. AEC containing FA and CBA reduces carbon footprint because of substitution of cement and it also helps to conserve natural resources by the use of CBA in place of fine aggregates.
Change management and digital transformation project success in SMEs located in the Democratic Republic of the Congo
Authors: Kala Kamdjoug Jean Robert 
Published: March 2023

PurposeManagerial practices are essential in the success of information technology (IT) projects of digital transformation (DT). However, the literature has not yet specified all these managerial practices. This study aims to address this gap by investigating the influence of change management on the success of IT projects of DT. Additionally, the author examines the consequences on SMEs in the economic context of the Democratic Republic of the Congo (DRC).Design/methodology/approachThis study draws on a research model that elucidates aspects of the resource-based view (RBV) framework, notably the transformation of human resources based on change management. This model demonstrates the relationship between change management, DT and IT project success, which facilitates the performance and resilience of SMEs. To empirically validate and test the developed research model, we gathered 299 responses from SME managers in the DRC through cross-sectional data collection using a structured questionnaire. The author performed statistical analyses using variance-based structural equation modeling (PLS-SEM) with the help of SmartPLS 3.0.FindingsThis paper reveals how SME managers can succeed in DT projects with the change management of human resources. Furthermore, it establishes that the success of IT projects of DT is an essential for enhancing the performance and resilience of SMEs in the DRC.Originality/valueThis study contributes to the information systems (IS) literature on developing countries by highlighting the DRC context. Little research deals with the success factors of DT projects and their organizational impact on SMEs in developing countries. This study thus enriches the IS literature by filling this void.