bluuebunny/crossref_metadata_2025_split huggingface dataset:
The sex differences of rheumatoid arthritis symptoms and activities in China: A cross-sectional study of 3374 patients
Authors: Zhong Linda LD , Wang Rongsheng , Zhu Qi , Cao Pei Hua , Lam Wai Ching , Jiang Ting , Zhang Yuan Yuan , Shen Jie , Su Xiao , Xue Luan , Mao Jianchun , Fang Yong Fei , Gao Ming Li , Hu Chun Rong , Peng Jiang Yun , Gu Ying , Wei Qianghua , Huang Runyue , Lyu Aiping , Liu Hongxia , He Dongyi 
Published: July 2023

Abstract

Background There are limited data studied the association between sex and rheumatoid arthritis (RA) symptoms and activities in China. Methods This is a cross-sectional study with recruitment of RA patients from 10 hospitals in China from 2014 to 2018. Patients were evaluated with the 28-joint Disease Activity Score based on erythrocyte sedimentation rate (DAS28-ESR), Simple Disease Activity Index (SDAI) for Rheumatoid Arthritis, Visual Analog Scale (VAS) scores, Revised American College of Rheumatology (ACR) Functional Classification of Global Functional Status, and vital signs. Mann-Whitney U and Chi-square tests were used to compare the sex differences. Ordinal logistic regression and linear regression models were used to estimate the odds ratio (OR) and adjusted for confounding factors of age and body mass index (BMI). Subgroup analyses were conducted to study the associations in RA patients with hypertension and diabetes. Results A total of 3374 RA patients were recruited. Ratio of female versus male was 4:1. After adjusting for age and BMI, significant sex differences were found in ACR functions, DAS28-ESR, SDAI, VAS, and C-reactive protein (CRP) levels. Patients with hypertension and diabetes accounted for 10% and 3% among all the RA patients, respectively. For RA patients with hypertension, significant association was found only between sex and CRP. Sex was not significantly associated with most clinical manifestations in diabetic RA patients except for level 2 of ACR functions of joints. Conclusion Our study provided epidemiological data of RA and its comorbidities in China. The results did not reach consensus to support substantial sex differences of RA symptoms and activities in Chinese populations.
Breast cancer occurring during pregnancy of Luminal A type overexpressed CXCL13
Authors: Nozaki Fumi , Nakanishi Yoko , Tanino Tomoyuki , Ochi Tomohiro , In Reika , Kajiura Yuka , Kida Kumiko , Takei Junko , Yoshida Atsushi , Kanomata Naoki , Kitano Atsuko , Yamauchi Hideko , Masuda Shinobu 
Published: July 2023

Abstract
Background
Breast cancer during pregnancy and lactation occurs in 2.6% of female patients aged < 45 years, and its incidence has been increasing. The pathogenesis and genomic profiles of pregnancy-associated breast cancer (PABC) remain unclear. We analyzed patients with breast cancer that occurred during pregnancy (PrBC) and compared the findings with those of patients with breast cancer that did not occur during pregnancy (non-PrBC).
Methods
We performed gene expression analyses (using microarray and qRT-PCR) of PrBC (8 and 22 samples, respectively) and non-PrBC (8 and 24 samples, respectively) groups, which were matched for age, hormone receptor expression, and HER2 expression.
Results
Microarray analysis showed that 355 genes were upregulated and 151 genes were downregulated in the luminal type PrBC group compared to those in the non-PrBC group. Chemokine, C-X-C motif chemokine ligand 13 (CXCL13) gene was the most upregulated in the PrBC group compared to that in the non-PrBC group (log2 fold-change = 101.2), especially in the luminal A-type cells (p = 0.016). This result was corroborated by the qRT-PCR analysis of microdissected cancer cells, which was conducted using formalin-fixed paraffin-embedded (FFPE) samples (p < 0.001). A negative correlation was observed between CXCL13 and estrogen receptor 1 (ESR1) mRNA expression levels in luminal A-type breast carcinoma (τ = -0.109; p < 0.001).
Conclusion
CXCL13, a chemokine that regulates the function of immune cells, was significantly overexpressed in PrBC cells. The expression levels of CXCL13 and estrogen receptor (ER) were inversely correlated.
Clinical observation of short-term efficacy of fenestration discectomy combined suturing the annulus for lumbar disc herniation
Authors: Bi Jingwei , Meng Bin , Chen Wenhao , Yang Wanliang , Pan Xin 
Published: July 2023

Abstract
Objectives:
To explore feasibility and short-term efficacy of suturing the annulus after discectomy for lumbar disc herniation.
Methods:229 cases operated with mono-segment discectomy were analyzed from June 2021 to December 2021,among them,133 cases operated with fenestration discectomy combined suturing the annulus and 96 cases operated with fenestration discectomy.The length of surgical incision,the operating time,blood loss,the average temperature for three days after surgery,duration of hospitalization after operation,preoperative and postoperative JOA score,visual analogue scale, MRI and DTI images were compared.
Results:
No significant difference was found between the two groups in the length of surgical incision,the operating time,blood loss,the average temperature for three days after surgery,duration ofhospitalization after operation.But significant differences could be observed in postoperative JOA score,VAS score,the same with preoperative and postoperative score in the same group,get a good rate of improvement surgery.Postoperative DTI images of sutured group is better than the control group.
Conclusion:
The symptoms of the lumbardisc herniation can be reliefed effectively by both methods,no significant difference could be found between them in the operating time,blood loss,etc.But after suturing,the ruptured annulus can instantly get the mechanical integrity,so that the nucleus is not easy to prolapse through the damaged annulus,and can reduce the rate of recurrent lumbar disc herniation. Furthermore,the function and stability of the spine in sutured patients can be reserved,owing to the more healthy nucleus pulposus has been kept.But the long-term efficacy of suturing the annulus still needs further follow-up.
Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells
Authors: Defossez Pierre Antoine , Yamaguchi Kosuke , Chen Xiaoying , Rodgers Brianna , Miura Fumihito , Bashtrykov Pavel , Ferry Laure , Kirsh Olivier , Laisné Marthe , Bonhomme Frédéric , Scelfo Andrea , Salinas-Luypaert Catalina , Ugur Enes , Arimondo Paola , Leonhardt Heinrich , Kanemaki Masato , Fachinetti Daniele , Jeltsch Albert , Ito Takashi 
Published: July 2023

Abstract
DNA methylation is an essential epigenetic chromatin modification, and its maintenance in mammals requires the protein UHRF1. It is yet unclear if UHRF1 functions solely by stimulating DNA methylation maintenance by DNMT1, or if it has important additional functions. Using degron alleles, we show that UHRF1 depletion causes a much greater loss of DNA methylation than DNMT1 depletion. This is not caused by passive demethylation as UHRF1-depleted cells proliferate more slowly than DNMT1-depleted cells. Instead, bioinformatics, proteomics and genetics experiments establish that UHRF1, besides activating DNMT1, interacts with DNMT3A and DNMT3B and promotes their activity. In addition, we show that UHRF1 antagonizes active DNA demethylation by TET2. Therefore, UHRF1 has non-canonical roles that contribute importantly to DNA methylation homeostasis; these findings have practical implications for epigenetics in health and disease.
Pyrazoline-based fluorescent probe: Synthesis, Characterization, Theoretical Simulation, and Detection of picric acid
Authors: Sharma Promila , Yusuf Mohamad , Malik Ashok Kumar 
Published: July 2023

Abstract
2-Pyrazoline containing benzothiazole ring 2-[1-(1,3-benzothiazol-2-yl)-5-(4-methoxyphenyl)-4,5-dihydro-1H-pyrazol-3-yl]phenol (BP) have been synthesized for the effective identification of picric acid over other competing nitro compounds using fluorescence technique. The as-prepared pyrazoline BP showed quenching efficiency as high as 82% comparative to other nitro aromatics and the limit of detection was found to be 1.1 μM. The possible mechanism with the quenched AP detection efficiency was based on fluorescence energy transfer and photoinduced electron transfer. Moreover, the observed results were supported by the optimized structures of the compounds using the DFT/B3LYP/6-311G/LanL2DZ method. Eventually, the pyrazoline derivative BP was further utilized for real water samples, showing recoveries in the 87-101.06% and RSD was less than 3%.
Determining the Localization of Intraparotid Lymph Nodes Using High-Resolution Ultrasound in Patients Without Systemic Disorders
Authors: BALKAN Elif POLAT , İNCEBEYAZ Burak , DENİZ Hatice Ahsen , DELANTONİ Antigoni , ORHAN Kaan 
Published: July 2023

Abstract
Purpose
The parotid gland is the largest of the head and neck's three major salivary glands. The location of existing pathologic lymph nodes in the gland and lymph node staging are crucial for the development of treatment options and the determination of treatment efficacy. The purpose of this study was to determine the location and number of intraparotid lymph nodes and to add to the existing body of knowledge. The second objective of our research is to compare the location of intraparotid lymph odes in the Turkish and Greek populations.
Methods
In this study, ultrasound images from US equipment at the Ankara University Faculty of Dentistry and the Thessaloniki University Faculty of Dentistry were retrospectively scanned. The study utilized images in which the probe is held longitudinally and the parotid is viewed.
Results
The locations with the most lymph nodes were 1A (21.5%) and 2A (21.5%), whereas 2A-B (4.2%) had the fewest lymph nodes. Men had a statistically significant higher incidence of lymph nodes than women. In addition, this study revealed a statistically significant correlation between age and lymph node location. This study employed two distinct populations, and the locations of the majority of lymph nodes demonstrated statistically significant differences.
Conclusion
Determining the location and number of lymph nodes in the parotid gland is essential for surgical intervention and early detection of a variety of pathologies.
Phenol as Proton Shuttle and Buffer for Lithium-mediated Ammonia Electrosynthesis
Authors: Chorkendorff Ib , Fu Xianbiao , Xu Aoni , Pedersen Jakob B. , Li Shaofeng , Sažinas Rokas , Zhou Yuanyuan , Andersen Suzanne (Zamany) , Saccoccio Saccoccio , Deissler Niklas H. , Mygind Jon , Kibsgaard Jakob , Vesborg Peter , Nørskov Jens 
Published: July 2023

Abstract
Ammonia is a crucial component in the production of fertilizers and various nitrogen-based compounds. Now, the lithium-mediated nitrogen reduction reaction (Li-NRR) has emerged as a promising approach for ammonia synthesis at ambient conditions. The proton shuttle plays a critical role in the proton transfer process during Li-NRR. However, the structure-activity relationship and design principles for effective proton shuttles have not yet been established in practical Li-NRR systems. Here, we propose a general procedure for verifying a true proton shuttle and established design principles for effective proton shuttles. We systematically evaluate several classes of proton shuttles in a continuous-flow reactor with hydrogen oxidation at the anode. Among the tested proton shuttles, phenol exhibits the highest Faradaic efficiency of 72 ± 3% towards ammonia, surpassing that of ethanol, which has been commonly used so far. Experimental investigations including operando isotope-labelled mass spectrometry proved the proton-shuttling capability of phenol. Further mass transport modeling sheds light on the mechanism.
Contradictions between a Quantum-Mechanical Model of the photon and the Detection Principle of FMCW Measurements
Authors: Malz André 
Published: July 2023

Abstract
This article presents a study that provides robust evidence supporting the continuity of the electromagnetic field, while highlighting contradictions between a quantum-mechanical model of the photon and the detection principle of Frequency-Modulated Continuous Wave (FMCW) measurements. The primary objective was to investigate the detection method of FMCW for extremely weak signals, reaching energy levels akin to photons. Through thorough examination, significant contradictions emerged, favoring classical field theory and challenging the notion of electromagnetic field quantization. To strengthen this claim, a carefully designed experiment was conducted to measure sub-photon energy levels. The comprehensive analysis of both theoretical insights and experimental data presents compelling evidence, demonstrating the untenability of a quantized model for the electromagnetic field. These findings have profound implications for understanding the continuity of the electromagnetic field, prompting further exploration in this field of research.
One cell-cycle early regulation of cell division axis by cortically localized factors mediates oriented cell division critical for embryo morphology
Authors: Terui Hiromu , Takatori Naohito 
Published: July 2023

Abstract

Cortically localized factors play major roles in regulating cell division orientation during oriented cell division. Posterior End Mark (PEM), a protein localized to the posterior vegetal structure called the centrosome-attracting body (CAB) and responsible for the anterior-posterior asymmetry of the 8-cell stage ascidian embryo, was thought to regulate the orientation of the cell division by attracting the centrosome toward the CAB at the 4-cell stage. However, this was not supported by analyses of the four-dimensional dynamics of the centrosome and the CAB. Here, we observed the positions of the centrosomes and the CAB by confocal microscopy and found that PEM attracts the centrosome to the CAB at the 2-cell stage, but not at the 4-cell stage. In the results, PEM regulated cell division orientations in anterior cells, which lack the CAB/PEM at the 4-cell stage, and the position of the CAB in the posterior cells. We propose a new model on how the 8-cell stage asymmetry is formed where the cell division orientation at the 4-cell stage results from the PEM-dependent shift of the division plane relative to the body axis at the 2-cell stage.
Passaging of gingival fibroblasts from periodontally healthy and diseased sites upregulates osteogenesis-related genes
Authors: Karlis Gerasimos D. , Schoenmaker Ton , Tsoromokos Nektarios , Veth Olaf E. , Loos Bruno G. , de Vries Teun J. 
Published: July 2023

Abstract

In order to investigate biological processes of the periodontium, in vitro primary cell models have been established. To study the biology of the gingiva, primary gingival fibroblast cell models are widely used. For such experiments, cells need to be expanded and passaged. A key assumption is that primary cells maintain most of their original characteristics they have in situ. The aim of this research is to explore the impact of early passaging on selected gene expression of human gingival fibroblast cells. For this purpose, gene expression from outgrowth of the resected tissues until the fourth passage was followed for 9 tissue samples, from both healthy and diseased sites. Micrographs were taken from the cultures, RNA was extracted from the samples of each passage and quantitative PCR was performed for selected genes representing various biological processes. Epithelial cells were present during the first outgrowth, but were no longer present in the second passage. Our results indicate that the morphology of the gingival fibroblast cells does not change with passaging and that passages 2 to 4 contain only gingival fibroblasts. Gene expression of IL-1β, M-CSF, TNF-α, TLR4, POSTN, and FAPα was unchanged by passaging, expression of IL-6 and TLR2 decreased due to passaging and expression of in particular the selected osteogenesis genes (ALP, RUNX2, Osteonectin, COL1A), OPG and MKI67 increased with passaging. Our results emphasize the importance of using the same passage in experiments that use primary gingival fibroblasts from various donors.
Nuclear factor erythroid 2-related factor 2 is Essential for Low-Normobaric Oxygen Treatment Afforded Blood-Brain Barrier Protection Following Ischemic Stroke
Authors: Ma Xiao Xiao , xie haiyi , Hou PinPin , Wang Xiaojing , Zhou Wei , Wang Zhenhong 
Published: July 2023

Abstract
Cerebral ischemia/reperfusion(I/R) injury increases blood-brain barrier (BBB) permeability, leading to hemorrhagic transformation and brain edema. Normobaric hyperoxia (NBO) is a routine clinical treatment strategy for this condition. However, its neuroprotective effects remain controversial. This study investigates the effect of different NBO concentrations on I/R injury and explores the involvement of the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in the underlying mechanism. A mouse middle cerebral artery occlusion (MCAO) model, and an oxygen and glucose deprivation (OGD) model of mouse brain microvascular endothelial cells (bEnd.3), were used to investigate the effect of NBO on I/R injury. Reactive oxygen species (ROS) inducer and Nrf2 siRNA were used to explore the mechanisms of Nrf2 pathway in the effect of NBO on cerebrovascular endothelial cells. In the early stage of MCAO, 40% O2 NBO exposure significantly improved blood perfusion in the ischemic area and effectively relieved BBB permeability, cerebral edema, cerebral injury, and neurological function after MCAO. In the OGD model of endothelial cells, 40% O2 NBO exposure significantly reduced cell apoptosis, inhibited ROS burst, reduced ER stress, upregulated the expression of tight junction proteins, and stabilized the permeability of endothelial cells. Blocking the Nrf2 pathway nullified the protective effect of 40% O2 NBO on endothelial cells after OGDNrf2. Finally, our study confirmed the low concentrations of NBO have a neuroprotective effect on I/R by activating the Nrf2 pathway in endothelial cells.
New insights into the role of Cutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders
Authors: Cross Maria Pol , Mir-Pedrol Júlia , Toloza Lorena , Knödlseder Nastassia , Güell Marc , Maroutti Julien , Zouboulis Christos C. , Fábrega María-José 
Published: July 2023

Abstract
Background: Cutibacterium acnes (C. acnes) is one of the most prevalent bacteria that forms the human skin microbiota. Specific phylotypes of C. acnes have been linked to acne vulgaris development, while others have been found in individuals with healthy skin. In this scenario, bacterial extracellular vesicles (EVs) play an interkingdom communication role with the host. The purpose of this study was to examine the impact of EVs generated by various phylotypes of C. acnes on inflammation and sebum production using different in vitro skin models.
Results: The main findings of this study reveal that the proteomic profile of the cargo embodied in the EVs reflects distinct characteristics of the different C. acnes phylotypes in terms of lifestyle, survival, and virulence. The in vitro skin models showed an extended pro-inflammatory modulation of A1 EVs consistently triggering the activation of the inflammation-related factors IL-8, IL-6, TNFαand GM-CSF, in comparison to H1 and H2. Additionally, an acne-prone skin model utilizing PCi-SEB and arachidonic acid (AA) as a sebum inductor, was employed to investigate the impact of C. acnes EVs on sebum regulation. The results indicated that all three types of EVs significantly inhibited sebum production after a 24-hour treatment period, with H1 EVs exhibiting the most pronounced inhibitory effect when compared to the positive control.
Conclusions: The results of this study highlight the protective nature of C. acnes H1 EVs and their potential as a natural treatment option for alleviating symptoms associated with inflammation and oily skin.
Optimization of thermosonication conditions for critical quality parameters of watermelon juice using response surface methodology
Authors: Maoto Makaepea M. , Jideani Afam I. O. , Maoto Makaepea Mossa , Jideani Afam I O 
Published: July 2023

Abstract

The topical consumer interest for natural, healthier, safer and nutritinal juice, has intrigued the search for innovative technologies that can minimize product degradation. In this regard, thermosonication has been proposed as a potential processing technology that can inactivate microorganisms, and enzymes and produce fresh-like products. In line with this, watermelon (Citrullus lanatus) juice is one of the common fruit juices that is desired by consumers due to its desirable color, pleasant odor, sweet taste and low calories. However, this fruit juice is highly perishable and is thermo-sensitive, it degrades quickly under thermal processing. Therefore, this study aimed to identify optimal thermosonication processing conditions to retain the critical quality parameters of watermelon juice. Response surface methodology (RSM) employing a central composite design was used to determine the effects of temperature (25–52ºC), processing time (2–10 min) and amplitude level (24–60 µm) to retain the quality parameters (total polyphenolic content, lycopene, β-carotene and ascorbic acid) of watermelon juice. The optimum conditions obtained were 125ºC, 2 min, and 24 µm and were experimentally validated. These conditions resulted in lycopene of 7.4 mg/100 g, β-carotene of 0.15 mg/100 g, ascorbic acid of 2.86 mg/100 g and total polyphenolic content of 21.32 mg/100 mg/ GAE with desirability of 0.81. The proposed model was adequate (p < 0.0001) with a satisfactory determination coefficient (R2) less than 0.8 for all phytochemicals. The proposed conditions provided better preservation of the quality characteristics of watermelon juice.