bluuebunny/crossref_metadata_2025_split huggingface dataset:
Future projection of climate extremes across contiguous northeast India and Bangladesh
Authors: Paul Ashesh Rudra , Maity Rajib 
Published: September 2023

AbstractIn recent times, India has experienced a significant increase in the frequency and intensity of extreme weather events, particularly in northeast India (NEI), an area known for its rich natural resources. Despite the geographic continuity of NEI and Bangladesh, previous studies have failed to consider their interconnectedness, resulting in an incomplete understanding of the situation. To bridge this gap, a comprehensive study encompassed the entire NEI, including West Bengal and Bangladesh (hereafter referred to as NEIB). This study examined climate extremes in NEIB, utilizing 12 temperature-based and 8 precipitation-based indices developed by the Expert Team on Climate Change Detection and Indices. Analysis was performed on temperature and precipitation data obtained from the India Meteorological Department and Bangladesh Meteorological Department covering the period 1981–2021. Additionally, climate projections from 14 Global Climate Models participating in the CMIP6 were incorporated for the period 2015–2100, considering four different Shared Socioeconomic Pathways (SSPs) scenarios. Findings revealed that under the SSP585 scenario, a substantial rise of 4 °C in maximum temperatures and 5.5 °C in minimum temperatures by the end of the twenty-first century. Warming indices, such as the summer days index, indicated an expected increase of 53 days, while the Warm spell days index was estimated to rise by approximately 2 days. Heavy precipitation days (R20mm) were projected to increase by up to 14 days, with a notable impact in Meghalaya. While the number of rainy days is expected to decrease, the overall magnitude of precipitation is anticipated to remain relatively stable. Notably, the Simple daily intensity index demonstrated a rise of 2.4 mm/day compared to the current baseline of 14.4 mm/day. These projected changes have significant ramifications for water resources, agriculture, health, and infrastructure in the region.
Phenotypic screening of signaling motifs that efficiently induce cell proliferation
Authors: Umene Kirato , Nagamune Teruyuki , Kawahara Masahiro 
Published: September 2023

AbstractSince cell proliferation is one of the fundamental cell fates, artificial control of cell proliferation based on a receptor-engineering approach is increasingly important in therapeutic and industrial applications. Since the signal transduction properties of cytokine receptors are greatly influenced by the amino acid sequence of tyrosine motifs, here we develop a phenotypic screening approach that can directly select cell proliferation-inducing tyrosine motifs from a synthetic library. In the tyrosine motif library, amino acid sequences around the tyrosine are randomized to attain diverse binding patterns of signaling molecules. Theoretically, engineered receptors with distinct tyrosine motifs would activate signaling molecules in diverse patterns. Thus, we investigated whether tyrosine motif sequences capable of inducing cell proliferation could be selected from the cellular library expressing the motif-engineered receptors. Consequently, the selected motifs induced similar levels of cell proliferation compared to the cytoplasmic signaling domain of a native receptor. The motif-screening system was applicable to cells that may differentiate or proliferate depending on cytokine signals. To our best knowledge, this is the first report demonstrating phenotypic screening of tyrosine motifs in living cells. Our approach would open up new possibilities in the field of artificial control of cell fate based on signal transduction engineering.
Leptin receptor gene deficiency minimally affects osseointegration in rats
Authors: Jolic Martina , Ruscsák Krisztina , Emanuelsson Lena , Norlindh Birgitta , Thomsen Peter , Shah Furqan A. , Palmquist Anders 
Published: September 2023

AbstractMetabolic syndrome represents a cluster of conditions such as obesity, hyperglycaemia, dyslipidaemia, and hypertension that can lead to type 2 diabetes mellitus and/or cardiovascular disease. Here, we investigated the influence of obesity and hyperglycaemia on osseointegration using a novel, leptin receptor-deficient animal model, the Lund MetS rat. Machined titanium implants were installed in the tibias of animals with normal leptin receptor (LepR+/+) and those harbouring congenic leptin receptor deficiency (LepR−/−) and were left to heal for 28 days. Extensive evaluation of osseointegration was performed using removal torque measurements, X-ray micro-computed tomography, quantitative backscattered electron imaging, Raman spectroscopy, gene expression analysis, qualitative histology, and histomorphometry. Here, we found comparable osseointegration potential at 28 days following implant placement in LepR−/− and LepR+/+ rats. However, the low bone volume within the implant threads, higher bone-to-implant contact, and comparable biomechanical stability of the implants point towards changed bone formation and/or remodelling in LepR−/− rats. These findings are corroborated by differences in the carbonate-to-phosphate ratio of native bone measured using Raman spectroscopy. Observations of hypermineralised cartilage islands and increased mineralisation heterogeneity in native bone confirm the delayed skeletal development of LepR−/− rats. Gene expression analyses reveal comparable patterns between LepR−/− and LepR+/+ animals, suggesting that peri-implant bone has reached equilibrium in healing and/or remodelling between the animal groups.
Multidimensional attributes expose Heider balance dynamics to measurements
Authors: Linczuk Joanna , Górski Piotr J. , Szymanski Boleslaw K. , Hołyst Janusz A. 
Published: September 2023

AbstractMost of studied social interactions arise from dyadic relations. An exception is Heider Balance Theory that postulates the existence of triad dynamics, which however has been elusive to observe. Here, we discover a sufficient condition for the Heider dynamics observability: assigning the edge signs according to multiple opinions of connected agents. Using longitudinal records of university student mutual contacts and opinions, we create a coevolving network on which we introduce models of student interactions. These models account for: multiple topics of individual student opinions, influence of such opinions on dyadic relations, and influence of triadic relations on opinions. We show that the triadic influence is empirically measurable for static and dynamic observables when signs of edges are defined by multidimensional differences between opinions on all topics. Yet, when these signs are defined by a difference between opinions on each topic separately, the triadic interactions’ influence is indistinguishable from noise.
Damping contribution of viscoelastic core on airborne sound insulation performance of finite constrained layer damping panels at low and middle frequencies
Authors: Wang Bo , Min Hequn 
Published: September 2023

AbstractThe airborne sound insulation performance of finite sandwich panels is often significantly worsened by resonant transmission components in low and middle frequencies. In this paper, damping contribution of viscoelastic core on sound transmission loss (STL) of finite constrained layer damping (CLD) panels is studied in narrow frequency bands. A fully coupled layer-wise approach is used with a generalized high-order shear deformation hypothesis that accounts for all types of deformations in the core. The influence of several parameters is investigated extensively. Results show that the adverse impact of the first-three odd-odd order modes, namely (1,1), (3,1), and (1,3) modes, as well as some higher-order modes on STL cannot be disregarded. The constrained viscoelastic core plays a crucial role in enhancing, or even eliminating, dips of STL spectrum at resonant frequencies. Additionally, it can considerably counterbalance a relatively broadband reduction of STL caused by the inter-modal coupling in middle frequencies. The damping mechanism can be divided into two aspects: (i) the reduction of modal amplitude by vibration energy dissipation, and (ii) the change of bending modal shapes. CLD treatment is a concise and effective way to achieve stable sound insulation performance.
Variations in medical practice of retinopathy of prematurity among 8 Asian countries from an international survey
Authors: Youn Young-Ah , Kim Sae Yun , Cho Su Jin , Chang Yun Sil , Miyake Fuyu , Kusuda Satoshi , Iskandar Adhi Teguh Perma , Rohsiswatmo Rinawati , Dewi Rizalya , Chee Seok Chiong , Neoh Siew Hong , Imperial Ma. Lourdes S. , Velasco Belen Amparo E. , Quek Bin Huey , Lin Yuh-Jyh , Chang Jui-Hsing , Nuntnarumit Pracha , Ngerncham Sopapan , Supapannachart Sarayut , Ozawa Yuri , Tomotaki Seiichi , Prempunpong Chatchay , Prempraphan Pathaporn , Isayama Tetsuya 
Published: September 2023

AbstractAdvances in perinatal care have led to the increased survival of preterm infants with subsequent neonatal morbidities, such as retinopathy of prematurity (ROP). This study aims to compare the differences of neonatal healthcare systems, resources, and clinical practice concerning ROP in Asia with review of current literature. An on-line survey at the institutional level was sent to the directors of 336 neonatal intensive care units (NICU) in 8 collaborating national neonatal networks through the Asian Neonatal Network Collaboration (AsianNeo). ROP screening was performed in infants born at < 34 weeks in Indonesia and Japan. In South Korea, Malaysia, and Taiwan, most screened for ROP in infants born at < 32 weeks. In all networks, majority of NICUs conducted ROP screening to infants with birth weight < 1500 g. In most NICU’s in-hospital ophthalmologists performed indirect ophthalmoscopy and some were supplemented with digital imaging. Both laser photocoagulation and anti-vascular endothelial growth factor injection are performed for treatment and, vitreous surgeries are conducted less frequently in all countries. Despite limited information collected by the survey, this first study to compare ROP practices implemented in eight Asian countries through AsianNeo will enable an understanding of the differences and facilitate quality improvement by sharing better practices.
Risk of age older than 65 years for 30-day cardiac complication may be comparable to low-to-moderate risk according to revised cardiac risk index in non-cardiac surgery
Authors: Oh Ah Ran , Park Jungchan , Lee Jong-Hwan , Cha Dahye , Choi Dan-Cheong , Yang Kwangmo , Ahn Joonghyun , Sung Ji Dong , Park I. Hyun , Lee Seung-Hwa 
Published: September 2023

AbstractRevised cardiac risk index (RCRI) is widely used for surgical patients without containing age as a risk factor. We investigated age older than 65 years with respect to low-to-moderate risk of RCRI. From January 2011 to June 2019, a total of 203,787 consecutive adult patients underwent non-cardiac surgery at our institution. After excluding high-risk patients defined as RCRI score > 2, we stratified the patients into four groups according to RCRI and age (A: age < 65 with RCRI < 2, [n = 148,288], B: age ≥ 65 with RCRI < 2, [n = 42,841], C: age < 65 with RCRI = 2, [n = 5,271], and D: age ≥ 65 with RCRI = 2, [n = 5,698]). Incidence of major cardiac complication defined as a composite of cardiac death, cardiac arrest and myocardial infarction was compared. After excluding 1,689 patients with high risk (defined as RCRI score > 2), 202,098 patients were enrolled. The incidence with 95% confidence interval of major cardiac complication for A, B, C, and D groups was 0.3% (0.2–0.3), 1.1% (1.0–1.2), 1.8% (1.6–1.8), and 3.1% (2.6–3.6), respectively. In a direct comparison between B and C groups, old patients with RCRI < 2 showed a significantly lower risk compared to younger patients with RCRI = 2 (odd ratio, 0.62; 95% confidence interval, 0.50–0.78; p < 0.001). In non-cardiac surgery, the risk of age older than 65 years was shown to be comparable with low-to-moderate risk according to RCRI.
Efficacy of copper nanoparticles encapsulated in soya lecithin liposomes in treating breast cancer cells (MCF-7) in vitro
Authors: Ahmed Shaimaa A. , Gaber Mohamed H. , Salama Aida A. , Ali Said A. 
Published: September 2023

AbstractCancer is one of the leading causes of death, which has attracted the attention of the scientific world to the search for efficient methods for treatment. With the great development and regeneration of nanotechnology over the last 25 years, various nanoparticles in different structures, shapes and composites provide good potential for cancer therapy. There are several drugs approved by FDA used in breast cancer treatment like Cyclophosphamide, Doxorubicin Hydrochloride, Femara, Herceptin, etc. Each has several side effects as well as treatment, which limits the use of drugs due to heart failure, pulmonary dysfunction, or immunodeficiency. Recently, such side effects are greatly reduced by using innovative delivery techniques. Some drugs have been approved for use in cancer treatment under the concept of drug delivery, such as Doxil (liposomal loaded doxorubicin). The purpose of this study is to investigate the effect of copper nanoparticles (CuNPs) as a drug model for cancer treatment, either in their free form or encapsulated in Soy lecithin liposomes (SLP) from plant origin as a cheap source of lipids. CuNPs were prepared by the chemical reduction method and loaded onto SLP through the thin film hydration method. The drug model Cu/SLP was successfully combined. The characteristics of the free CuNPs, liposomes, and the combined form, zeta potential, size distribution, drug encapsulation efficiency (EE%), drug release profile, Fourier transform infrared (FTIR), and transmission electron microscopy (TEM), were checked, followed by an in vitro study on the breast cancer cell line Mcf-7 as a model for cytotoxicity evaluation. The optimal Cu/SLP had a particle mean size of 81.59 ± 14.93 nm, a negative zeta potential of − 50.7 ± 4.34 mV, loaded CuNPs showed an EE% of 78.9%, a drug release profile for about 50% of the drug was released after 6 h, and FTIR analysis was recorded. The cytotoxicity assay showed that the IC50 of Cu/SLP is smaller than that of free CuNPs. These results give clear evidence of the efficacy of using the combined Cu/SLP rather than CuNPs alone as a model drug carrier prepared from plant origin against cancer, both medically and economically.
Optimization of vertically aligned carbon nanotube beam trajectory with the help of focusing electrode in the microchannel plate
Authors: Adhikari Bishwa Chandra , Ketan Bhotkar , Patil Ravindra , Choi Eun Ha , Park Kyu Chang 
Published: September 2023

AbstractThe focusing electrode plays an important role to reduce the electron beam trajectory with low dispersion and high brightness. This article summarizes the importance of the vertically aligned multi-walled carbon nanotube effect with the focusing electrode. First of all, the effect of electron beam trajectory is studied with the different heights, hole sizes, and applied voltage of the focusing electrode by the opera 3D simulation. The field emission electron beam spot is captured in the microchannel plate which helps to reduce the signal noise effect and damage of CNT tips by the joule heating effect. The high-dense bright spot is optimized at the focusing electrode hole size of 2 mm, and the height of 1 mm from the gate mesh electrode at the low bias voltage of − 200 V without the loss of current. The FWHM of the electron beam is calculated 0.9 mm with its opening angle of 0.9° which could be applicable in high-resolution multi-electron beam microscopy and nano-focused X-ray system technology.
Two successive outbreaks of acute gastroenteritis due to norovirus GII.6 in a holiday camp house
Authors: Alsedà Miquel , Godoy Pere , Bach Pilar , Soldevila Núria , Cornejo Thais , Corominas Laura , Grau Maria , Domínguez Àngela ,   The Working Group for the Study of Outbreaks of Acute Gastroenteritis in Catalonia, Alsedà Miquel , Álvarez Josep , Barrabeig Irene , Belver Anna Isabel , Camps Neus , Minguell Sofia , Carol Monica , Godoy Pere , Izquierdo Conchita , Jané Mireia , Martínez Ana , Parrón Ignacio , Pérez Cristina , Rovira Ariadna , Sabaté Maria , Sala Maria Rosa , Torner Núria , Vileu Rosa Maria , de Andres Anna , de Benito Javier , Camprubí Esteve , Cunillé Montse , Forns M. Lluïsa , Moreno-Martínez A. , Razquín Efrén , Rius Cristina , Sabaté Sara , de Simón Mercé , Rodríguez Virginia , Bartolomé Rosa , Cornejo Thais , Guix Susana , Coronas Lorena , Domínguez Àngela , Soldevila Núria 
Published: September 2023

AbstractWhen two outbreaks occur in the same institution within a short period of time, an important health and social concern is generated. Two gastroenteritis outbreaks occurring a week apart in the same facility were reported in Lleida, Spain, in 2018. The objective of this study was to describe the clinical, epidemiological and microbiological investigation carried out and to determine the risk factors. Demographic data, food consumption and symptoms were collected. Health inspections of the facility were carried out. Risk ratio and their 95% confidence intervals were estimated for the implication of each food consumed. The attack rate was 89.7% in the first outbreak and 69.6% in the second outbreak. The most frequent symptoms in the first and second outbreak were abdominal pain (88.5% and 100%, respectively), vomiting (80.8% and 87.5%, respectively) and nausea (69.2% and 81.3%, respectively). The first outbreak was associated with the consumption of a salad and the second with a cheese omelet. Norovirus GII.6 was detected by RT-PCR and sequenced in both groups of students and in the food handlers who prepared the meals. These results highlight the importance of exclusion from work of food handlers with gastroenteritis, the adequate availability of mechanisms for correct hand washing and the correct cleaning of surfaces.
Statistical optimization, characterization, antioxidant and antibacterial properties of silver nanoparticle biosynthesized by saw palmetto seed phenolic extract
Authors: Abdel-Aty Azza M. , Barakat Amal Z. , Bassuiny Roqaya I. , Mohamed Saleh A. 
Published: September 2023

AbstractOn the global market, silver nanoparticles (Ag-NPs) are in high demand for their various applications in biomedicine, material engineering, and consumer products. This study highlighted the biosynthesis of the Ag-NPs using saw palmetto seed phenolic extract (SPS-phenolic extract), which contained vital antioxidant-phenolic compounds. Herein, central composite statistical design, response surface methodology, and sixteen runs were conducted to optimize Ag-NPs biosynthesis conditions for maximizing the production of Ag-NPs and their phenolic content. The best-produced SPS-Ag-NPs showed a surface plasmon resonance peak at 460 nm and nano-spherical sizes ranging from 11.17 to 38.32 nm using the UV spectrum analysis and TEM images, respectively. The produced SPS-Ag-NPs displayed a high negative zeta-potential value (− 32.8 mV) demonstrating their high stability. The FTIR analysis demonstrated that SPS-phenolic compounds were involved in sliver bio-reduction and in stabilizing, capping, and preventing Ag-NP aggregation. The thermogravimetric investigation revealed that the produced SPS-Ag-NPs have remarkable thermal stability. The produced SPS-Ag-NP exceeded total antioxidant activity (13.8 µmol Trolox equivalent) more than the SPS-phenolic extract (12.0 µmol Trolox equivalent). The biosynthesized SPS-Ag-NPs exhibited noticeably better antibacterial activity against multidrug-resistant Gram-negative E. coli and Gram-positive S. aureus compared to SPS-phenolic extract. Hence, the bio-synthesized SPS-Ag-NPs demonstrated great potential for use in biomedical and antimicrobial applications.
Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells
Authors: Yoshida Kazuma , Suzuki Shigeki , Yuan Hang , Sato Akiko , Hirata-Tsuchiya Shizu , Saito Masahiro , Yamada Satoru , Shiba Hideki 
Published: September 2023

AbstractDental pulp stem cells (DPSC) usually remain quiescent in the dental pulp tissue; however, once the dental pulp tissue is injured, DPSCs potently proliferate and migrate into the injury microenvironment and contribute to immuno-modulation and tissue repair. However, the key molecules that physiologically support the potent proliferation and migration of DPSCs have not been revealed. In this study, we searched publicly available transcriptome raw data sets, which contain comparable (i.e., equivalently cultured) DPSC and mesenchymal stem cell data. Three data sets were extracted from the Gene Expression Omnibus database and then processed and analyzed. MXRA5 was identified as the predominant DPSC-enriched gene associated with the extracellular matrix. MXRA5 is detected in human dental pulp tissues. Loss of MXRA5 drastically decreases the proliferation and migration of DSPCs, concomitantly with reduced expression of the genes associated with the cell cycle and microtubules. In addition to the known full-length isoform of MXRA5, a novel splice variant of MXRA5 was cloned in DPSCs. Recombinant MXRA5 coded by the novel splice variant potently induced the haptotaxis migration of DPSCs, which was inhibited by microtubule inhibitors. Collectively, MXRA5 is a key extracellular matrix protein in dental pulp tissue for maintaining the proliferation and migration of DPSCs.
Identification and validation of a prognostic risk-scoring model based on the level of TIM-3 expression in acute myeloid leukemia
Authors: Huang Wanxue , Zheng Shasha , Wang Qi , Zhao Na , Long Zhiguo 
Published: September 2023

AbstractAcute myeloid leukemia (AML) is characterized by an unfavorable prognosis due to the presence of self-renewing leukemic stem cells (LSCs). The presence of T-cell immunoglobulin mucin-3 (TIM-3) on the surface of LSCs has been observed in various types of human AML, exerting an impact on the prognostic outcome. Exploring the hub genes associated with varying levels of TIM-3 expression offers a valuable approach to enhance our understanding of the underlying mechanisms involving TIM-3 and to identify potential prognostic indicators in AML. Nevertheless, to date, no research studies have reported a prognostic model that relies on the level of TIM-3 expression. In our study, we screen the hub-genes based on different expression level of TIM-3 through WGCNA. The prognostic risk-scoring model was constructed based on hub-genes. The results show the risk prognostic model has extraordinary ability to predict prognosis in both the training and validation sets. The high-risk group present poor prognosis with mutation of NPM1, TP53 (Multiple Hit) and FLT3(multiple hit), while IDH2 (Missense Mutation), MUC16 (Multiple Hit/Missense Mutation) occur mutation in low-risk group presenting favorite prognosis than high-risk group. Leukocyte cell–cell adhesion, regulation of T cell activation and I-κB kinase/NF-κB signaling enriched in high-risk group, involving in HSCs or LSCs anchoring to BM, which implicated in LSCs survival and chemotherapy resistance. B7-H3 (CD276) and CD276 would be the potential immune targets in high-risk group. The risk score model may help in distinguishing immune and molecular characteristics, predicting patient outcomes.