bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomics identifies differentially expressed proteins in glioblastoma U87 cells treated with hederagenin
Authors: Zhang Yesen , Han Yi , Shang Yuchun , Wang Xiangyu , Sun Jiwei 
Published: April 2023

AbstractObjectiveWe investigated differentially expressed proteins (DEPs) in human glioblastoma U87 cells after treatment with hederagenin as a therapeutic screening mechanism and provided a theoretical basis for hederagenin in treating glioblastoma.MethodsThe Cell Counting Kit 8 assay was used to analyze the inhibitory effect of hederagenin on the proliferation of U87 cells. Protein was identified by tandem mass tags and LC-MS/MS analysis techniques. Annotation of DEPs, Gene Ontology enrichment and function, and Kyoto Encyclopedia of Genes and Genomes pathways and domains were all examined by bioinformatics. According to the TMT results, hub protein was selected from DEPs for WB verification.ResultsProtein quantitative analysis found 6522 proteins in total. Compared with the control group, 43 DEPs (P < 0.05) were involved in the highly enriched signaling pathway in the hederagenin group, among which 20 proteins were upregulated, and 23 proteins were downregulated. These different proteins are mainly involved in the longness regulating pathway–WORM, the hedgehog signaling pathway,Staphylococcus aureusinfection, complement, coagulation cascades, and mineral absorption. KIF7 and ATAD2B expression were significantly down-regulated and PHEX and TIMM9 expression were significantly upregulated, according to WB analysis, supporting the TMT findings.ConclusionHederagenin inhibition of GBM U87 cells may be related to KIF7, which is mainly involved in the hedgehog signaling pathway. Our findings lay a foundation for additional study of the therapeutic mechanism of hederagenin.
Esophageal papillomatosis: an exceedingly rare disease
Authors: Li Dandan , Li Changfeng , Yan Yuxing , Liu Minya 
Published: April 2023

AbstractIf esophageal papilloma (EP) is a rare condition, esophageal papillomatosis (EPS) is a distinct rarity. To date, only 53 well documented cases have been described in English literature. However, the number of reports on EPS significantly increased to over 40 cases during the past 20 years. Perhaps, this is due to the broad use of endoscopy and related research achievements. Most of the cases are individual and it seems that there are no associations between them. And up to now no guidelines can be followed. To further understand this exceedingly rare disease, we had a comprehensive review of the epidemiology, etiology, clinical manifestations, pathogenesis, treatment, and clinical course of EPS.
Seizures provoked by over-the-counter cough and cold medications in an elderly patient: a case report
Authors: Alattiya Mohammed Kays , Joudeh Anwar I. , Hammamy Riyadh Ali 
Published: April 2023

Abstract
Background
Seizures are common neurological emergencies in the elderly that are frequently provoked. Geriatrics have higher rates of neurological disorders and other comorbidities that could affect seizure threshold.

Case presentation
An 83-year-old male Arabic patient presented to the emergency department with an acute confusional state and urinary incontinence followed by a witnessed tonic–clonic seizure in the hospital. Thorough investigations and imaging were positive only for nonspecific magnetic resonant imaging findings with a negative electroencephalogram. The patient was diagnosed with provoked seizure due to over-the-counter cold medications that included pseudoephedrine and caffeine. He was not prescribed antiepileptic medications at discharge and did not develop subsequent seizures up to this date.

Conclusion
Over-the-counter cough and cold medications with sympathomimetic ingredients can be associated with provoked seizures in the elderly. Physicians should be aware of the potentially serious adverse events associated with commonly used nonprescription sympathomimetics such as pseudoephedrine and caffeine in elderly patients.
Meloxicam inhibits STING phosphorylation and alleviates intracellular DNA-mediated autoimmune responses
Authors: Yu Yu , Wang Miao , Li Xiao-Wei , Mao Jie , Zhu Ying-Jie , Wang Na , Yin Le-Hua , Guo Zeng-Lin , Cai Hong , Li Tao , Liang Ting-Ting , Cui Jiuwei , Zhou Tao 
Published: April 2023

Abstract
Background
Cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway is critical for cytosolic DNA-sensing and the subsequent immune responses. The inappropriate activation of this pathway leads to DNA-induced autoimmune response. Understanding the precise regulation of cGAS-STING pathway is important for developing therapeutics to treat several autoimmune diseases caused by self-DNA.

Results
We report that Meloxicam (MXC) inhibits intracellular DNA-, but not RNA-induced immune responses. We find that MXC inhibits the phosphorylation of STING by examining in different cells with various DNA stimulations. We further find that MXC significantly dampens the expression levels of interferon-stimulated genes (ISGs) by using DNA 3’ repair exonuclease 1 (TREX1)-deficient cell, an experimental model for self-DNA-induced autoimmune disease. Importantly, we demonstrate that MXC could promote the survival in Trex1–/– mouse model for Aicardi-Goutières syndrome (AGS).

Conclusions
Our study identified a non-steroidal anti-inflammatory drug, MXC, that exhibits potential effect in treating the autoimmunity caused by self-DNA.
Pathogen-based target attainment of optimized continuous infusion dosing regimens of piperacillin-tazobactam and meropenem in surgical ICU patients: a prospective single center observational study
Authors: De Corte Thomas , Verhaeghe Jarne , Dhaese Sofie , Van Vooren Sarah , Boelens Jerina , G. Verstraete Alain , Stove Veronique , Ongenae Femke , De Bus Liesbet , Depuydt Pieter , Van Hoecke Sofie , J. De Waele Jan 
Published: April 2023

Abstract
Background
Several studies have indicated that commonly used piperacillin-tazobactam (TZP) and meropenem (MEM) dosing regimens lead to suboptimal plasma concentrations for a range of pharmacokinetic/pharmacodynamic (PK/PD) targets in intensive care unit (ICU) patients. These targets are often based on a hypothetical worst-case scenario, possibly overestimating the percentage of suboptimal concentrations. We aimed to evaluate the pathogen-based clinically relevant target attainment (CRTA) and therapeutic range attainment (TRA) of optimized continuous infusion dosing regimens of TZP and MEM in surgical ICU patients.

Methods
A single center prospective observational study was conducted between March 2016 and April 2019. Free plasma concentrations were calculated by correcting total plasma concentrations, determined on remnants of blood gas samples by ultra-performance liquid chromatography with tandem mass spectrometry, for their protein binding. Break points (BP) of identified pathogens were derived from epidemiological cut-off values. CRTA was defined as a corrected measured total serum concentration above the BP and calculated for increasing BP multiplications up to 6 × BP. The upper limit of the therapeutic range was set at 157.2 mg/L for TZP and 45 mg/L for MEM. As a worst-case scenario, a BP of 16 mg/L for TZP and 2 mg/L for MEM was used.

Results
781 unique patients were included with 1036 distinctive beta-lactam antimicrobial prescriptions (731 TZP, 305 MEM) for 1003 unique infections/prophylactic regimens (750 TZP, 323 MEM). 2810 samples were available (1892 TZP, 918 MEM). The median corrected plasma concentration for TZP was 86.4 mg/L [IQR 56.2–148] and 16.2 mg/L [10.2–25.5] for MEM. CRTA and TRA was consistently higher for the pathogen-based scenario than for the worst-case scenario, but nonetheless, a substantial proportion of samples did not attain commonly used PK/PD targets.

Conclusion
Despite these pathogen-based data demonstrating that CRTA and TRA is higher than in the often-used theoretical worst-case scenario, a substantial proportion of samples did not attain commonly used PK/PD targets when using optimised continuous infusion dosing regimens. Therefore, more dosing optimization research seems warranted. At the same time, a ‘pathogen-based analysis’ approach might prove to be more sensible than a worst-case scenario approach when evaluating target attainment and linked clinical outcomes.
A dynamic few-shot learning framework for medical image stream mining based on self-training
Authors: Ye Zhengqiang , Zhang Wei 
Published: May 2023

AbstractFew-shot semantic segmentation (FSS) has been widely used in the field of information medicine and intelligent diagnosis. Due to the high cost of medical data collection and the privacy protection of patients, labeled medical images are difficult to obtain. Compared with other semantic segmentation dataset which can be automatically generated in a large scale, the medical image data tend to be continually generated. Most of the existing FSS techniques require abundant annotated semantic classes for pre-training and cannot deal with its dynamic nature of medical data stream. To deal with this issue, we propose a dynamic few-shot learning framework for medical semantic segmentation, which can fully utilize the features of newly-collected/generated data stream. We introduce a new pseudo-label generation strategy for continuously generating pseudo-labels and avoiding model collapse during self-training. Furthermore, an efficient consistency regularization strategy is proposed to fully utilize the limited data. The proposed framework is iteratively trained on three tasks: abdominal organ segmentation for CT and MRI, and cardiac segmentation for MRI. Experiments results demonstrate significant performance gain on medical data stream mining compared with the baseline method.
Efficacy and safety of carnitine supplementation on NAFLD: a systematic review and meta-analysis
Authors: Liu Aiping , Cai Yitong , Yuan Yuan , Liu Ming , Zhang Zhengjing , Xu Yongquan , Jiao Pingzu 
Published: April 2023

Abstract
Background and objective
The efficacy and safety of L-carnitine supplementation on non-alcoholic fatty liver disease (NAFLD) are unclear. This systematic review and meta-analysis aimed to assess the efficacy and safety of L-carnitine supplementation on NAFLD.

Methods
We searched in four databases (PubMed, Embase, Cochrane Library, and Web of Science) from inception to 1 November 2022 (updated on March 20, 2023) for potentially relevant records without language restrictions. We collected information on the first author, publication year, country, setting, study design, population characteristics, duration of follow-up, outcome variables of interest, and sources of funding. We used a modified Cochrane risk of bias tool to assess the risk of bias, used GRADE to assess the certainty of evidence, and used the Credibility of Effect Modification Analyses (ICEMAN) tool to assess the credibility of any apparent subgroup effect.

Results
This systematic review and meta-analysis included eight eligible randomized controlled trials (RCTs). Compared to placebo, low certainty evidence show that L-carnitine supplementation significantly changes (reduced) more in AST levels and ALT levels (MD: − 26.38, 95%CI: − 45.46 to − 7.30), and moderate certainty evidence show that L-carnitine supplementation significantly changes (reduced) more in HDL cholesterol levels (MD: 1.14, 95%CI: 0.21 to 2.07) and triglyceride levels (MD: − 6.92, 95%CI: − 13.82 to − 0.03). Moderate credibility of ICEMAN results shows that L-carnitine supplementation has no difference in changes of AST and ALT levels in younger ones (MD: 0.5, 95%CI: − 0.70 to 1.70) but has significant changes (reduced) in adults (MD: − 20.3, 95%CI: − 28.62 to − 12.28) compared to placebo.

Conclusion
L-carnitine supplementation may improve liver function and regulate triglyceride metabolism in patients with NAFLD, and with no significant adverse effects.
A novel candidate gene CLN8 regulates fat deposition in avian
Authors: Li Xiaoqin , Zhang Fan , Sun Yunxiao , Sun Dandan , Yang Fangxi , Liu Yongtong , Hou Zhuocheng 
Published: May 2023

Abstract
Background
The fat deposition has a crucial role in animal meat flavor, and fat deposition-related traits are vital for breeding in the commercial duck industry. Avian fat-related traits are typical complex phenotypes, which need a large amount of data to analyze the genetic loci.

Results
In this study, we performed a new phenotypic analysis of fat traits and genotyped whole-genome variations for 1,246 ducks, and combed with previous GWAS data to reach 1,880 ducks for following analysis. The carcass composition traits, subcutaneous fat weight (SFW), subcutaneous fat percentage (SFP), abdominal fat weight (AFW), abdominal fat percentage (AFP) and the body weight of day 42 (BW42) for each duck were collected. We identified a set of new loci that affect the traits related to fat deposition in avian. Among these loci, ceroid-lipofuscinosis, neuronal 8 (CLN8) is a novel candidate gene controlling fat deposition. We investigated its novel function and regulation in avian adipogenesis. Five significant SNPs (the most significant SNP, P-value = 21.37E−12) and a single haplotype were detected in the upstream of CLN8 for subcutaneous fat percentage. Subsequently, luciferase assay demonstrated that 5 linked SNPs in the upstream of the CLN8 gene significantly decreased the transcriptional activity of CLN8. Further, ATAC-seq analysis showed that transcription factor binding sites were identified in a region close to the haplotype. A set of luciferase reporter gene vectors that contained different deletion fragments of the CLN8 promoter were constructed, and the core promoter area of CLN8 was finally identified in the −1,884/−1,207 bp region of the 5′ flanking sequences, which contains adipogenesis-related transcription factors binding sites. Moreover, the over-expression of CLN8 can remarkably facilitate adipocyte differentiation in ICPs. Consistent with these, the global transcriptome profiling and functional analysis of the over-expressed CLN8 in the cell line further revealed that the lipid biosynthetic process during the adipogenesis was significantly enriched.

Conclusions
Our results demonstrated that CLN8 is a positive regulator of avian adipocyte differentiation. These findings identify a novel function of CLN8 in adipocyte differentiation, which provides important clues for the further study of the mechanism of avian fat deposition.
Multi-omics analysis reveals gut microbiota-ovary axis contributed to the follicular development difference between Meishan and Landrace × Yorkshire sows
Authors: Xu Baoyang , Qin Wenxia , Chen Yuwen , Tang Yimei , Zhou Shuyi , Huang Juncheng , Ma Libao , Yan Xianghua 
Published: May 2023

Abstract
Background
The mechanism by which Meishan (MS) sows are superior to white crossbred sows in ovarian follicle development remains unclear. Given gut microbiota could regulate female ovarian function and reproductive capacity, this study aimed to determine the role of gut microbiota-ovary axis on follicular development in sows.

Methods
We compared the ovarian follicular development, gut microbiota, plasma metabolome, and follicular fluid metabolome between MS and Landrace × Yorkshire (L × Y) sows. A H2O2-induced cell apoptosis model was used to evaluate the effects of multi-omics identified metabolites on the apoptosis of porcine ovarian granulosa cells in vitro.

Results
Compared with L × Y sows, MS sows have greater ovary weight and improved follicular development, including the greater counts of large follicles of diameter ≥ 5 mm, secondary follicles, and antral follicles, but lesser atretic follicles. The ovarian granulosa cells in MS sows had alleviated apoptosis, which was indicated by the increased BCL-2, decreased caspases-3, and decreased cleaved caspases-3 than in L × Y sows. The ovarian follicular fluid of MS sows had higher concentrations of estradiol, progesterone, follicle-stimulating hormone, luteinizing hormone, and insulin like growth factor 1 than L × Y sows. Gut microbiota of MS sows formed a distinct cluster and had improved alpha diversity, including increased Shannon and decreased Simpson than those of L × Y sows. Corresponding to the enhanced function of carbohydrate metabolism and elevated short-chain fatty acids (SCFAs) in feces, the differential metabolites in plasma between MS and L × Y sows are also mainly enriched in pathways of fatty acid metabolism. There were significant correlations among SCFAs with follicular development, ovarian granulosa cells apoptosis, and follicular fluid hormones, respectively. Noteworthily, compared with L × Y sows, MS sows had higher follicular fluid SCFAs concentrations which could ameliorate H2O2-induced porcine granulosa cells apoptosis in vitro.

Conclusion
MS sows have more secondary and antral follicles, but fewer atretic follicles and apoptotic ovarian granulosa cells, as well as harbored a distinctive gut microbiota than L × Y sows. Gut microbiota may participate in regulating ovarian follicular development via SCFAs affecting granulosa cells apoptosis in sows.
Material analysis for restoration application: a case study of the world’s first university Mor Yakup Church in Nusaybin, Mardin
Authors: Karataş Lale , Alptekin Aydın , Yakar Murat 
Published: May 2023

Abstract
The Mor Yakup Church, located in the Nusaybin District of Mardin, is known as the world’s first educational university in history and represents one of the oldest Christian medieval monuments. In this study, it is aimed to determine the factors of the strength problems of the structure by investigating the characterization of building materials and what kind of factors affect the material behavior with various observational and experimental methods. It was determined that the main deterioration types in the materials of the building were erosion, fractures, loss of parts and the dissolve of the joint mortars between the masonry work on the facades. Since the materials used in the construction of the building are unable in terms of physico-mechanics, it has been determined that the severe continental climate conditions prevailing in the region easily cause such physical deterioration on the construction materials. In addition, the presence of clays in the conventional mortar used in the building has been defined as an internal problem that causes the material to get tired with the osmotic pressure it creates by absorbing water. A very high rate of salinization was detected in the building materials of the building and it was observed that this salting was caused by the acid effect caused by air pollution and the portland cement used in the previous repairs in the building. Finally, this study presents restoration recommendations to repair the material deterioration in the building and to prevent its occurrence in the future.
Interdisciplinary Infection Prevention and Control Bundle in Neurosurgical Patients: Results of a Prospective Cohort Study
Authors: Koch Julia , Saydan Selin , Schwab Frank , Sanchin Aminaa , Hansen Sonja , Trampuz Andrej , Margaryan Donara , Vajkoczy Peter , Onken Julia Sophie 
Published: May 2023

BACKGROUND:
Surgical site infections (SSIs) account for one of the most common causes of nosocomial infections. Bundle approaches for infection prevention and control do not capture the full complexity of neurosurgical interventions.

OBJECTIVE:
To study the efficacy of an interdisciplinary infection prevention and control bundle (IPCB) in neurosurgery.

METHODS:
This was a prospective, single-center, observational study, analyzing 3 periods: before (2014), during (2017), and after (2019) full implementation of IPCB. IPCB included the following infection prevention measures: preoperative decolonization, patient engagement, operating room (OR) hygiene protocol, and pre-, peri-, and postoperative standard operating procedures (SOPs) while infection control measures included intraoperative sonication, blood culture inoculation, and interdisciplinary SSI management. All neurosurgical patients being readmitted to the hospital for SSIs within 90 days after receiving index surgery were included in the trial (403/9305).

RESULTS:
Implementation of IPCB resulted in more frequently succeeded pathogen isolation in patients with SSI (2014: 138 isolates in 105 (83%) patients with SSI, 2017: 169 isolates in 124 (91%) patients with SSI, and 2019: 199 isolates in 136 (97%) patients with SSI; P < .001). Proportion of gram-positive SSI and virulence was declining (P = .041, P = .007). The number of repeated revision surgeries decreased from 26 (20%) in 2014 and 31 (23%) in 2017 to 18 (13%) in 2019 (P = .085). Significantly, fewer patients experienced sepsis in response to SSI (2014: 12%, 2017: 10%, and 2019: 3.6%, P = .035). In-hospital mortality rate was declining from 12 (9.4%) in 2014 to 9 (6.6%) in 2017 to 5 (3.6%) in 2019 (P = .148).

CONCLUSION:
Introducing an interdisciplinary IPCB in neurosurgery leads to a significant reduction of sepsis and decreased in-hospital mortality while a pathogen switch toward gram-negative bacteria was observed. Minimizing diagnostic gap of pathogen detection toward a more efficient anti-infective treatment may be the main reason for the substantial decrease in morbidity and mortality.
breakthrough in acute coronary syndrome diagnostics – transdermal troponin assessment
Authors: Rejmer Adrian , Szala-Czerwonka Karolina , Woś Natalia , Rojek Katarzyna , Bednarz Lucjan , Wijas Karolina , Bialic Kinga , Bakalarczyk Rafał , Bialic Artur , Majewski Paweł 
Published: April 2023

Introduction and purpose:
The measurement of cardiac troponins, besides ECG and basic patient examination, is a vital component of the diagnosis and management of patients with acute coronary syndrome (ACS), including unstable angina (UA), non-ST and ST elevation myocardial infarction (NSTEMI, STEMI). Traditionally cardiac troponins have been measured through blood samples, but a new method of transdermal wrist measurement has emerged as a promising alternative. The potential benefits of transdermal troponin measurement are numerous. First and foremost, the non-invasive nature of the method means that patients can be diagnosed “on the spot” and monitored in real time without the need for repeated blood draws, vastly reducing the time for diagnosis and patient qualification for invasive treatment, percutaneous coronary intervention - PCI, if needed. The coronary syndrome is the number 1 cause of death in developed countries which includes morbidity and mortality. With the invention of transdermal troponin assessment device, we can increase the life expectancy and the quality of life in the group of patients with acute coronary syndrome and most importantly, decrease the total turnaround time of diagnosis and significantly reduce the workload in the emergency department.
Displaced distal tibial Salter-Harris II fractures
Authors: Peterson Nick , Perry Daniel C. 
Published: May 2023

Salter-Harris II fractures of the distal tibia affect children frequently, and when they are displaced present a treatment dilemma. Treatment primarily aims to restore alignment and prevent premature physeal closure, as this can lead to angular deformity, limb length difference, or both. Current literature is of poor methodological quality and is contradictory as to whether conservative or surgical management is superior in avoiding complications and adverse outcomes. A state of clinical equipoise exists regarding whether displaced distal tibial Salter-Harris II fractures in children should be treated with surgery to achieve anatomical reduction, or whether cast treatment alone will lead to a satisfactory outcome. Systematic review and meta-analysis has concluded that high-quality prospective multicentre research is needed to answer this question. The Outcomes of Displaced Distal tibial fractures: Surgery Or Casts in KidS (ODD SOCKS) trial, funded by the National Institute for Health and Care Research, aims to provide this high-quality research in order to answer this question, which has been identified as a top-five research priority by the British Society for Children’s Orthopaedic Surgery.Cite this article: Bone Joint J 2023;105-B(5):471–473.