bluuebunny/crossref_metadata_2025_split huggingface dataset:
Label‐Free Fluorescence Turn‐On Detection of Histidine‐Tagged Proteins Based on Intramolecular Rigidification Induced Emission
Authors: Pu Chibin , Huang Zhongxi , Huang Lihua , Shen Qian , Yu Changmin 
Published: March 2023

AbstractHistidine tag (His‐tag) is a representative epitope tag, which has been widely used in the purification or immobilization of recombinant proteins. In this study, a novel probeCTPE‐IDA‐Ni2+based on intramolecular rigidification induced emission for fluorescence detecting His‐tagged proteins is successfully developed and experimentally demonstrated. This detecting system is based on TPE derivative incorporated IDA‐Ni2+moiety for real‐time detection of His‐tagged proteins. In the presence of His‐tagged proteins, the IDA‐Ni2+‐His‐tag complex was formed, which restricted the rotation of two phenyl rings linked with TPE and subsequently resulting in the fluorescence turn on. Results have shown that our probe could directly detect His‐tagged proteins with good selectivity and fast response within 10 min. In addition, we have proved that this system could be used in fluorescent labeling of His‐tagged protein instead of traditional Western blot assay with simple procedure, high quality and low background interference, as well as without aggregation‐caused quenching (ACQ) problems like some organic dyes.
Synthesis, DFT Calculations, and Molecular Docking Study of Acetohydrazide‐Based Sulfonamide Derivatives as Paraoxonase 1 Inhibitors
Authors: Arslan Gülnur , Gökçe Başak , Muhammed Muhammed Tilahun , Albayrak Özlem , Önkol Tijen , Özçelik Azime Berna 
Published: March 2023

AbstractParaoxonase 1 (PON1), an esterase linked to high‐density lipoprotein (HDL), is known to have strong antioxidant and anti‐cardiovascular properties. In this study, eleven acetohydrazide‐based sulfonamide derivatives were synthesized. All compounds were characterized by appropriate spectroscopic techniques and elemental analyses. To better understand the inhibitory properties of the new sulfonamide derivatives, theirin vitroeffects on purified PON1 enzyme activity were studied. For this purpose, PON1 was purified from human serum using simple chromatographic methods. In the experimental study, the novel compounds were found to be potent inhibitors of paraoxonase 1. The mode of binding to the enzyme, for the relatively active compounds, was investigated through molecular docking. The most active compound N′‐(naphthalen‐2‐ylsulfonyl)‐2‐(2‐oxo‐1,3‐benzothiazol‐3(2H)‐yl)acetohydrazide demonstrated the highest interaction with the enzyme. Furthermore, the electrochemical properties of these compounds were assessed through DFT (density functional theory) analysis. N′‐(naphthalen‐2‐ylsulfonyl)‐2‐(2‐oxo‐1,3‐benzothiazol‐3(2H)‐yl)acetohydrazide is anticipated to have the highest potential to take part in electron exchanges whereas N′‐[(4‐fluorophenyl)sulfonyl]‐2‐(2‐oxo‐1,3‐benzoxazol‐3(2H)‐yl)acetohydrazide is expected to have the highest chemical stability.
Growth of La‐Doped ZnO Thin Films on a Porous Silicon Substrate for Photocatalytic Applications
Authors: Atyaoui Malek , Hamouda Faten , Ezzaouia Hatem 
Published: March 2023

AbstractThe aim of this work was to improve photo‐catalytic activities of zinc oxide thin films for the degradation of methyl orange (MO) using a new approach based on the combined effect of lanthanum doping and porous substrate of silicon (Psi). Therefore, undoped and lanthanum‐doped zinc oxide thin films (ZnO : La) were deposited on porous silicon substrate (PSi) by spin coating chemical deposition. Studies of the photo‐catalytic activity showed that the La (25 %)‐doped ZnO layer exhibited an improved photocatalytic performance in the degradation of methyl orange. Around 91.53 % and 70 % of the MO degradation was achieved within 180 min under illumination with UV and visible light respectively, suggesting that La‐doped ZnO films deposited on Psi substrate are favorable candidates to enhance the photo‐degradation of methyl orange.
Extending the Library of 4‐Hydroxycoumarin Derivatives with Potential Pharmacological Activity by a Catalyst‐Free and Purification‐Free Method
Authors: Rey Apostol Romal John , Kee Ong Say 
Published: March 2023

AbstractConstant mutations of SARS‐CoV‐2 from 2019 to the present commences potential dilemma to the efficacy of developed COVID‐19 antiviral drugs. In this current report, series of 3‐phenyl(alkylamino)methyl‐4‐hydroxycoumarin was identified as compounds of drug‐likeliness that, through molecular docking simulations, also demonstrated favourable binding to different sites of the SARS‐CoV‐2 RBD/hACE2 complex. This was achieved by varying the chain of the alkyl length and the substituent of the phenyl moiety. The subjected 3‐phenyl(alkylamino)methyl‐4‐hydroxycoumarin compounds were successfully synthesized by a catalyst‐free multicomponent condensation reaction of 4‐hydroxycoumarin, p‐substituted benzaldehyde, and linear alkyl amines in dichloromethane (DCM) at 22 °C. The crude products were achieved in a moderate (40–50 %) to a very good (80–90 %) yield with excellent purity without the need for chemical purification verified by nuclear magnetic resonance (NMR) spectroscopy. The simple method to produce 3‐phenyl(alkylamino)methyl‐4‐hydroxycoumarin and the preliminary docking results present an opportunity for advancement in drug discovery.
Ageing and physical resilience after health stressors
Authors: Colon‐Emeric Cathleen , Schmader Kenneth , Cohen Harvey Jay , Morey Miriam , Whitson Heather 
Published: March 2023

AbstractThe objectives of this article are to introduce a conceptual framework for physical resilience in the context of ageing and to discuss key elements and challenges in the design of studies of physical resilience after health stressors. Advancing age is associated with increasing exposure to multiple stressors and declining capacity to respond to health stressors. Resilience is defined broadly as the ability to resist or recover well from the adverse effects of a health stressor. In ageing‐related study designs of physical resilience after a health stressor, this dynamic resilience response can be observed as changes in repeated measures of function or health status in various domains important to older adults. Methodologic issues in selecting the study population, defining the stressor, covariates, outcomes, and analytic strategies are highlighted in the context of an ongoing prospective cohort study of physical resilience after total knee replacement surgery. The article concludes with approaches to intervention development to optimize resilience.
Emerging applications of green coconut: A promising lignocellulosic biomass
Authors: Sharma Ritu , Mittal Mahak , Yadav Anita , Aggarwal Neeraj K. 
Published: March 2023

AbstractWaste accumulation is a grave concern and becoming a transboundary challenge for environment. During Covid‐19 pandemic, diverse type of waste were collected due to different practices employed in order to fight back the transmission rate of the virus. Covid‐19 was proved to be capricious catastrophe of this 20th century and even not completely eradicated from the world. The havoc created by this imperceptible quick witted, pleomorphic deadly virus can't be ignored. Though a number of vaccines have been developed by the scientists but there is a fear of getting this virus again in our life. Medical studies prove that immunity drinks will help to reduce its reoccurrences. Coconut water is widely used among all drinks available globally. Its massive consumption created an incalculable pile of green coconut shells around the different corners of the world. This practice generating enormous problem of space acquisition for the environment. Both the environment and public health will benefit from an evaluation of quantity of coconut waste that is being thrown and its potential to generate value added products. With this context, present article has been planned to study different aspects like, coconut waste generation, its biological properties and environmental hazards associated with its accumulation. Additionally, this review illustrates, green technologies for production of different value added products from coconut waste.
The Afromontane Forest and Montane Grassland Biome
Authors: Huntley Brian John 
Published: March 2023

AbstractThe most vulnerable, yet biogeographically important forests of Angola are the relict patches of Afromontane forests of the highlands. This Chapter describes these ‘islands in the sky’ and their threatened endemic species. The small patches, totaling less than 10 km2 in area, lie from 2000–3000 km distant from their closest related forests in East, Southern and West Africa. The physical conditions, floristic and faunistic composition and biogeographic significance of the Afromontane forests and grasslands are described.
The Angolan Escarpment Zone
Authors: Huntley Brian John 
Published: March 2023

AbstractThe Angolan Escarpment is a physical feature that forms part of the Great Escarpment of Southern Africa. It comprises elements of all the major biomes of Angola. This chapter focuses on the biogeographical importance of the Escarpment Zone, which runs south from the Maiombe forests of Cabinda to the arid mountains rising above the Cunene River.
Tree level amplitudes from soft theorems
Authors: Zhou Kang 
Published: March 2023

Abstract
We demonstrate that the tree level amplitudes and the explicit formulas of soft factors can be uniquely determined by soft theorems and the universality of soft factors. By imposing the soft theorems and the universality, as well as the assumption of double copy, we reconstruct single trace Yang-Mills-scalar amplitudes and pure Yang-Mills amplitudes, in the expanded formulas. The explicit formulas of soft factors for the bi-adjoint scalar and gluon are also determined. The expansions of Yang-Mills-scalar and Yang-Mills amplitudes can be extended to Einstein-Yang-Mills and gravitational amplitudes, and we use the expanded single trace Einstein-Yang-Mills amplitudes to reproduce the soft factors for the graviton.
Unique alcohol dehydrogenases involved in algal sugar utilization by marine bacteria
Authors: Brott Stefan , Nam Ki Hyun , Thomas François , Dutschei Theresa , Reisky Lukas , Behrens Maike , Grimm Hanna C. , Michel Gurvan , Schweder Thomas , Bornscheuer Uwe T. 
Published: March 2023

Abstract
Marine algae produce complex polysaccharides, which can be degraded by marine heterotrophic bacteria utilizing carbohydrate-active enzymes. The red algal polysaccharide porphyran contains the methoxy sugar 6-O-methyl-d-galactose (G6Me). In the degradation of porphyran, oxidative demethylation of this monosaccharide towards d-galactose and formaldehyde occurs, which is catalyzed by a cytochrome P450 monooxygenase and its redox partners. In direct proximity to the genes encoding for the key enzymes of this oxidative demethylation, genes encoding for zinc-dependent alcohol dehydrogenases (ADHs) were identified, which seem to be conserved in porphyran utilizing marine Flavobacteriia. Considering the fact that dehydrogenases could play an auxiliary role in carbohydrate degradation, we aimed to elucidate the physiological role of these marine ADHs. Although our results reveal that the ADHs are not involved in formaldehyde detoxification, a knockout of the ADH gene causes a dramatic growth defect of Zobellia galactanivorans with G6Me as a substrate. This indicates that the ADH is required for G6Me utilization. Complete biochemical characterizations of the ADHs from Formosa agariphila KMM 3901T (FoADH) and Z. galactanivorans DsijT (ZoADH) were performed, and the substrate screening revealed that these enzymes preferentially convert aromatic aldehydes. Additionally, we elucidated the crystal structures of FoADH and ZoADH in complex with NAD+ and showed that the strict substrate specificity of these new auxiliary enzymes is based on a narrow active site.

Key points
• Knockout of the ADH-encoding gene revealed its role in 6-O-methyl-D-galactose utilization, suggesting a new auxiliary activity in marine carbohydrate degradation.
• Complete enzyme characterization indicated no function in a subsequent reaction of the oxidative demethylation, such as formaldehyde detoxification.
• These marine ADHs preferentially convert aromatic compounds, and their strict substrate specificity is based on a narrow active site.
Feasibility, safety, and efficacy of early prophylactic donor lymphocyte infusion after T cell-depleted allogeneic stem cell transplantation in acute leukemia patients
Authors: van der Zouwen Boris , Koster E. A. S. , von dem Borne P. A. , Oosten L. E. M. , Roza-Scholten M. W. I. , Snijders T. J. F. , van Lammeren D. , van Balen P. , Marijt W. A. F. , Veelken H. , Falkenburg J. H. F. , de Wreede L. C. , Halkes C. J. M. 
Published: March 2023

AbstractProphylactic donor lymphocyte infusion (DLI) starting at 6 months after T cell-depleted allogeneic stem cell transplantation (TCD-alloSCT) can introduce a graft-versus-leukemia (GvL) effects with low risk of severe graft-versus-host-disease (GvHD). We established a policy to apply low-dose early DLI at 3 months after alloSCT to prevent early relapse. This study analyzes this strategy retrospectively. Of 220 consecutive acute leukemia patients undergoing TCD-alloSCT, 83 were prospectively classified to have a high relapse risk and 43 were scheduled for early DLI. 95% of these patients received freshly harvested DLI within 2 weeks of the planned date. In patients transplanted with reduced intensity conditioning and an unrelated donor, we found an increased cumulative incidence of GvHD between 3 and 6 months after TCD-alloSCT for patients receiving DLI at 3 months compared to patients who did not receive this DLI (0.42 (95%Confidence Interval (95% CI): 0.14–0.70) vs 0). Treatment success was defined as being alive without relapse or need for systemic immunosuppressive GvHD treatment. The five-year treatment success in patients with acute lymphatic leukemia was comparable between high- and non-high-risk disease (0.55 (95% CI: 0.42–0.74) and 0.59 (95% CI: 0.42–0.84)). It remained lower in high-risk acute myeloid leukemia (AML) (0.29 (95% CI: 0.18–0.46)) than in non-high-risk AML (0.47 (95% CI: 0.42–0.84)) due to an increased relapse rate despite early DLI.
Randomized, placebo-controlled study on efficacy, safety and tolerability of drug-induced defibrinogenation for sudden sensorineural hearing loss: the lessons learned
Authors: Weiss Bernhard G. , Spiegel Jennifer L. , Becker Sven , Strieth Sebastian , Olzowy Bernhard , Bertlich Mattis , Fořt Tomáš , Mejzlik Jan , Lenarz Thomas , Ihler Friedrich , Canis Martin 
Published: March 2023

Abstract
Purpose
Disturbance of cochlear microcirculation is discussed as final common pathway of various inner ear diseases. Hyperfibrinogenemia causing increased plasma viscosity is a possible factor for a critical reduction of cochlear blood flow that might lead to sudden sensorineural hearing loss (SSHL). The aim was to determine the efficacy and safety of drug-induced defibrinogenation by ancrod for SSHL.

Methods
Double-blind, randomized, placebo-controlled, multicenter, parallel group, phase II (proof-of-concept) study (planned enrollment: 99 patients). Patients received an infusion of ancrod or placebo (day 1) followed by subcutaneous administrations (day 2, 4, 6). Primary outcome was the change in pure tone audiogram air conduction average until day 8.

Results
The study was terminated early due to slow recruiting (31 enrolled patients: 22 ancrod, 9 placebo). A significant improvement of hearing loss was registered in both groups (ancrod: − 14.3 dB ± 20.4 dB, − 39.9% ± 50.4%; placebo: − 22.3 dB ± 13.7 dB, − 59.1% ± 38.0%). A statistically significant group-difference was not detected (p = 0.374). Placebo response of 33.3% complete and 85.7% at least partial recovery was observed. Plasma fibrinogen levels were reduced significantly by ancrod (baseline: 325.2 mg/dL, day 2: 107.2 mg/dL). Ancrod was tolerated well, no adverse drug reaction was of severe intensity, no serious adverse events occurred.

Conclusion
Ancrod reduced fibrinogen levels that support its mechanism of action. The safety profile can be rated positively. Since the planned number of patients could not be enrolled, no efficacy conclusion can be drawn. The high rate of placebo response challenges clinical trials for SSHL and needs to be considered in future investigations.
Trial registrations
This study was registered in the EU Clinical Trials Register, EudraCT-No. 2012-000066-37 at 2012-07-02.
Oncological outcome of vocal cord-only radiotherapy for cT1-T2 glottic laryngeal squamous cell carcinoma
Authors: de Ridder Mischa , Rijken Johannes A. , Smits Hilde J. G. , Smid Ernst J. , Doornaert Patricia A. H. , de Bree Remco 
Published: March 2023

Abstract
Purpose
Early-stage glottic cancer can be treated with radiotherapy only. Modern radiotherapy solutions allow for individualized dose distributions, hypofractionation and sparing of organs at risk. The target volume used to be the entire voice box. This series describe the oncological outcome and toxicity of individualized vocal cord-only hypofractionated radiotherapy for early stage (cT1a-T2 N0).

Methods
Retrospective cohort study with patients treated in a single center between 2014 and 2020.

Results
A total of 93 patients were included. Local control rate was 100% for cT1a, 97% for cT1b and 77% for cT2. Risk factor for local recurrence was smoking during radiotherapy. Laryngectomy-free survival was 90% at 5 years. Grade III or higher late toxicity was 3.7%.

Conclusion
Vocal cord-only hypofractionated radiotherapy appears to be oncologically safe in early-stage glottic cancer. Modern, image-guided radiotherapy led to comparable results as historical series with very limited late toxicity.
Measuring the Optimal Time Interval Between Arrival and First Mental Health Evaluation’s for Refugees in Québec: A Scoping Review
Authors: Maillet Lara , Goudet Anna , Godbout Isabelle , Ntanda Gisèle Mandiangu , Laliberté Geneviève , Desjardins France , Benoit Maryse , Vassiliadis Helen-Maria , Loignon Christine , Manceau Luiza Maria 
Published: March 2023

AbstractTo map the state of the existing literature to identify the optimal time frame between the arrival of refugees in a host country and psychosocial assessments. We conducted scoping review using the method of Arksey and O’Malley (2005). A systematic search of 5 databases including PubMed, Psycinfo (OVID), PsycINFO BD APA, Scopus and Web of Sciences) and grey literature identified 2698 references. Thirteen studies published between 2010 and 2021 were considered eligible. A data extraction grid was designed and tested by the research team. It is not so ease to identify the most appropriate time interval to assess the mental health of newly settled refugees. All the studies selected agree on the need to carry out an initial assessment when refugees arrive in their host country. Several authors agree on the need to carry out screening at least twice during the resettlement period. However, what is less clear is the best time to perform the second screening. This scoping review mainly helped in highlighting the lack of probing data on the mental health indicators focused on during the assessment and on the optimal timeline for the assessment of refugees. Further research is needed to determine whether developmental and psychological screening is beneficial, the right time to perform the screening, and the most appropriate collection instruments and interventions.
A binational social vulnerability index (BSVI) for the San Diego-Tijuana region: mapping trans-boundary exposure to climate change for just and equitable adaptation planning
Authors: Rosa Melissa , Haines Kyle , Cruz Teddy , Forman Fonna 
Published: March 2023

AbstractTo pursue just, inclusive, and participatory climate adaptation planning and policy, it is important to understand both regional climate trends and the ecological services that reduce vulnerability and exposure to climate risks at the community level. Rapidly growing cities like Tijuana and San Diego are doubly exposed to climate change because they have fewer resources to confront them and yet responsible for basic services that support everyday life of their residents, challenges that are complicated by the divided institutional and social context of an international border region. In the binational context, the regional community is fragmented by institutional, academic, and cultural factors, leading to adaptation planning that stops at the border despite the shared ecological setting of human settlements. This fragmentation is particularly dangerous for climate adaptation planning because it obscures inequalities as well as opportunities contained in the binational region. To address this deficit, we have synthesized information from a variety of regional spatial datasets to construct a continuous binational social vulnerability index (BSVI) at the census tract level across the San Diego-Tijuana border region. This paper details the datasets and methodology used to create the BSVI and explores some of the preliminary results of the analysis by juxtaposing this score with spatially explicit information on vegetation cover and climate projections of heat and rainfall extremes across the region. We close with a discussion on use of this research as a tool for local environmental justice and regional adaptation.
The effects of pH on nutrient availability depend on both soils and plants
Authors: Barrow N. J. , Hartemink Alfred E. 
Published: March 2023

Abstract
The effects of pH on nutrient availability are not solely caused by to the effects on reaction with soils but are an interaction between these effects and the effects on rate of uptake by plants. Some effects are specific to particular ions, but an important aspect is that plant roots and soil particles both have variable charge surfaces. This influences availability, but in opposite directions. Sulfate is an example of this interplay. Its sorption by soil decreases markedly with increasing pH and thus “soil availability” increases. However, plant uptake also decreases with increasing pH thus “plant availability” decreases. For phosphate, the plant effect is stronger than the soil effect and uptake decreases with increasing pH. In contrast, effects of increasing pH on molybdate adsorption are so large that they dominate the overall effect. Sorption of cations, such as zinc or copper, increases with increasing pH but uptake rate also increases. The net effect is a small decrease in availability with increasing pH. Boron is an exception; there are small effects of pH on sorption; and it is the uncharged boric acid molecules that are taken up by plant roots. Their uptake is not affected by charge and uptake is proportional to the concentration of uncharged boric acid molecules. We argue that emphasis on the effects of pH on reactions with soil has led to a distorted picture of the effects of pH on nutrient availability.
Patchworks and operations
Authors: Novick Rose , Haueis Philipp 
Published: March 2023

AbstractRecent work in the philosophy of scientific concepts has seen the simultaneous revival of operationalism and development of patchwork approaches to scientific concepts. We argue that these two approaches are natural allies. Both recognize an important role for measurement techniques in giving meaning to scientific terms. The association of multiple techniques with a single term, however, raises the threat of proliferating concepts (Hempel, 1966). While contemporary operationalists have developed some resources to address this challenge, these resources are inadequate to account for the full range of complex behaviors of scientific concepts. We adopt show how the patchwork approach’s repertoire of inter-patch relations can expand the resources available to the operationalist. We focus on one especially important type of inter-patch relation: sharing a general reasoning strategy. General reasoning strategies serve two important functions: (1) they bind together distinct patches of scientific concepts, and (2) they provide normative guidance for extending concepts to new domains.