bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA H19 Inhibits Keratinocyte Cell Proliferation and Migration by Targeting miR-17-5p/RUNX1 Axis in Chronic Wounds
Authors: Ji Wei , Zhang Qian , Sun Zhibo , Cheng Yanyang 
Published: September 2023

Abstract
The migration and proliferation of keratinocytes are critical for re-epithelization during chronic wound healing. Runt-related transcription factor 1 (RUNX1) has been indicated to repress keratinocyte proliferation. Nonetheless, the potential molecular mechanism of RUNX1 in regulating keratinocyte proliferation and migration remains unclear. Cell counting kit-8 and wound-healing assays were implemented for examining keratinocyte viability and migration, respectively. Western blotting and real-time quantitative polymerase chain reaction were utilized for quantifying protein and RNA levels. Luciferase reporter assay was employed for verifying the interaction between RUNX1, miR-17-5p, and long noncoding RNA H19. The results showed that RUNX1 depletion promoted keratinocyte proliferation and migration and repressed extracellular matrix degradation. Mechanistically, H19 upregulated RUNX1 expression by competitively absorbing miR-17-5p. Rescue experiments revealed that overexpressing RUNX1 reversed H19 silencing-mediated effects on the phenotypes of keratinocytes. In conclusion, H19 knockdown promotes keratinocyte proliferation and migration and suppresses extracellular matrix degradation via the miR-17-5p/RUNX1 axis.
Close competitors? Bilateral bank competition and spatial variation in firms’ access to credit
Authors: De Haas Ralph , Lu Liping , Ongena Steven 
Published: September 2023

Abstract
We interviewed 379 bank CEOs in 20 emerging markets to identify their banks’ main competitors. We show that banks are more likely to identify another bank as a main competitor in small-business lending when both banks are foreign owned or relationship oriented; when there exists a large spatial overlap in their branch networks and when the potential competitor has fewer hierarchical layers. We then construct a novel bilateral competition measure at the locality level and assess how well it explains geographic variation in firms’ credit constraints. We show that intense bilateral bank competition tightens local credit constraints, especially for small firms, as competition may impede the formation of lending relationships.
Abolishing storage lipids induces protein misfolding and stress responses inYarrowia lipolytica
Authors: Zaghen Simone , Konzock Oliver , Fu Jing , Kerkhoven Eduard J 
Published: September 2023

AbstractYarrowia lipolytica naturally saves excess carbon as storage lipids. Engineering efforts allow redirecting the high precursor flux required for lipid synthesis toward added-value chemicals such as polyketides, flavonoids, and terpenoids. To redirect precursor flux from storage lipids to other products, four genes involved in triacylglycerol and sterol ester synthesis (DGA1, DGA2, LRO1, and ARE1) can be deleted. To elucidate the effect of the deletions on cell physiology and regulation, we performed chemostat cultivations under carbon and nitrogen limitations, followed by transcriptome analysis. We found that storage lipid-free cells show an enrichment of the unfolded protein response, and several biological processes related to protein refolding and degradation are enriched. Additionally, storage lipid-free cells show an altered lipid class distribution with an abundance of potentially cytotoxic free fatty acids under nitrogen limitation. Our findings not only highlight the importance of lipid metabolism on cell physiology and proteostasis, but can also aid the development of improved chassy strains of Y. lipolytica for commodity chemical production.
Single-cell transcriptomics reveal different maturation stages and sublineage commitment of human thymic invariant natural killer T cells
Authors: Maas-Bauer Kristina , Köhler Natalie , Stell Anna-Verena , Zwick Melissa , Acharya Swati , Rensing-Ehl Anne , König Christoph , Kroll Johannes , Baker Jeanette , Koßmann Stefanie , Pradier Amandine , Wang Sisi , Docquier Mylène , Lewis David B , Negrin Robert S , Simonetta Federico 
Published: September 2023

Abstract
Invariant natural killer T cells are a rare, heterogeneous T-cell subset with cytotoxic and immunomodulatory properties. During thymic development, murine invariant natural killer T cells go through different maturation stages differentiating into distinct sublineages, namely, invariant natural killer T1, 2, and 17 cells. Recent reports indicate that invariant natural killer T2 cells display immature properties and give rise to other subsets, whereas invariant natural killer T1 cells seem to be terminally differentiated. Whether human invariant natural killer T cells follow a similar differentiation model is still unknown. To define the maturation stages and assess the sublineage commitment of human invariant natural killer T cells during thymic development, in this study, we performed single-cell RNA sequencing analysis on human Vα24+Vβ11+ invariant natural killer T cells isolated from thymocytes. We show that these invariant natural killer T cells displayed heterogeneity, and our unsupervised analysis identified 5 clusters representing different maturation stages, from an immature profile with high expression of genes important for invariant natural killer T cell development and proliferation to a mature, fully differentiated profile with high levels of cytotoxic effector molecules. Evaluation of expression of sublineage-defining gene sets revealed mainly cells with an invariant natural killer T2 signature in the most immature cluster, whereas the more differentiated ones displayed an invariant natural killer T1 signature. Combined analysis with a publicly available single-cell RNA sequencing data set of human invariant natural killer T cells from peripheral blood suggested that the 2 main subsets exist both in thymus and in the periphery, while a third more immature one was restricted to the thymus. Our data point to the existence of different maturation stages of human thymic invariant natural killer T cells and provide evidence for sublineage commitment of invariant natural killer T cells in the human thymus.
Mitochondrial aldehyde dehydrogenase rescues against diabetic cardiomyopathy through GSK3β-mediated preservation of mitochondrial integrity and Parkin-mediated mitophagy
Authors: Zhang Yingmei , Zou Rongjun , Abudureyimu Miyesaier , Liu Qiong , Ma Jipeng , Xu Haixia , Yu Wei , Yang Jian , Jia Jianguo , Qian Sanli , Wang Haichang , Yang Yang , Wang Xin , Fan Xiaoping , Ren Jun 
Published: September 2023

Abstract
Mitochondrial aldehyde dehydrogenase (ALDH2) offers proven cardiovascular benefit, although its impact on diabetes remains elusive. This study examined the effects of ALDH2 overexpression and knockout on diabetic cardiomyopathy and the mechanism involved with a focus on mitochondrial integrity. Mice challenged with streptozotocin (STZ, 200 mg/kg, via intraperitoneal injection) exhibited pathological alterations, including reduced respiratory exchange ratio, dampened fractional shortening and ejection fraction, increased left ventricular end-systolic and diastolic diameters, cardiac remodeling, cardiomyocyte contractile anomalies, intracellular Ca2+ defects, myocardial ultrastructural injury, oxidative stress, apoptosis, and mitochondrial damage, which were overtly attenuated or accentuated by ALDH2 overexpression or knockout, respectively. Diabetic patients also exhibited reduced plasma ALDH2 activity, cardiac remodeling, and diastolic dysfunction. In addition, STZ challenge altered expression levels of mitochondrial proteins (PGC-1α and UCP2) and Ca2+ regulatory proteins (SERCA, Na+–Ca2+ exchanger, and phospholamban), dampened autophagy and mitophagy (LC3B ratio, TOM20, Parkin, FUNDC1, and BNIP3), disrupted phosphorylation of Akt, GSK3β, and Foxo3a, and elevated PTEN phosphorylation, most of which were reversed or worsened by ALDH2 overexpression or knockout, respectively. Furthermore, the novel ALDH2 activator torezolid, as well as the classical ALDH2 activator Alda-1, protected against STZ- or high glucose-induced in vivo or in vitro cardiac anomalies, which was nullified by inhibition of Akt, GSK3β, Parkin, or mitochondrial coupling. Our data discerned a vital role for ALDH2 in diabetic cardiomyopathy possibly through regulation of Akt and GSK3β activation, Parkin mitophagy, and mitochondrial function.
Different demographic responses of three species of container Aedes (Diptera: Culicidae) larvae to timing of extrinsic mortality
Authors: Juliano Steven A , McIntire Kristina M , Nichols Hannah O , Canizela Cecilia C , Frederick Kaitlyn M 
Published: September 2023

Abstract
Mortality imposed on a population can interact with negatively density-dependent mortality to produce overcompensation, wherein added mortality results in more survivors. Experimental mortality can cause overcompensation in mosquito larvae, which would be counterproductive if it resulted from mosquito control in nature. We tested for different demographic responses to mortality among 3 container Aedes species when impacted by density dependence. We imposed 48.2% mortality on cohorts of larvae 2, 6, or 8 days after hatching and compared adult production, development times, and female size to those variables for controls without mortality. Mortality significantly increased adult production compared to controls, but the 3 species varied in the details of that response. Aedes albopictus (Skuse) produced more adults with mortality on day 2 primarily because of greater production of males. Aedes triseriatus (Say) yielded more adults with mortality on day 2 primarily because of greater production of females. Aedes aegypti (L.) adult production was not significantly affected by mortality, but development times for both sexes were significantly shorter with mortality on day 8. There were no effects of mortality on female wing length. None of our mortality treatments yielded significant reductions of adults for any species. These species responses to mortality are not the same, despite their similar ecologies and life histories. Thus, we cannot assume that killing almost half the larvae present in a dense population will reduce adult production, nor can we assume that different Aedes species will respond to mortality in the same way.
NFYB increases chemosensitivity in glioblastoma by promoting HDAC5-mediated transcriptional inhibition of SHMT2
Authors: Zhang Yingfan , Huang Haoxuan , Liu Peikun , Xie Yuanyang 
Published: September 2023

Abstract
Temozolomide (TMZ) is a commonly used chemotherapeutic agent for glioblastoma (GBM), but acquired drug resistance prevents its therapeutic efficacy. We investigated potential mechanisms underlying TMZ resistance and glycolysis in GBM cells through regulation by nuclear transcription factor Y subunit β (NFYB) of the oncogene serine hydroxymethyltransferase 2 (SHMT2). GBM U251 cells were transfected with NFYB-, SHMT2-, and the potential NFYB target histone deacetylase 5 (HDAC5)-related vectors. Glucose uptake and lactate production were measured with detection kits. CCK-8/colony formation, scratch, Transwell, and flow cytometry assays were performed to detect cell proliferation, migration, invasion, and apoptosis, respectively. The binding of NFYB to the HDAC5 promoter and the regulation of NFYB on HDAC5 promoter activity were detected with chromatin immunoprecipitation and dual-luciferase reporter assays, respectively. NFYB and HDAC5 were poorly expressed and SHMT2 was expressed at high levels in GBM U251 cells. NFYB overexpression or SHMT2 knockdown decreased glucose uptake, lactate production, proliferation, migration, and invasion and increased apoptosis and TMZ sensitivity of the cells. NFYB activated HDAC5 to inhibit SHMT2 expression. SHMT2 overexpression nullified the inhibitory effects of NFYB overexpression on glycolysis and TMZ resistance. Thus, NFYB may reduce tumorigenicity and TMZ resistance of GBM through effects on the HDAC5/SHMT2 axis.
Attention-Deficit Hyperactivity Disorder Symptoms, Underperception of Respiratory Compromise, and Illness Representations in Black and Latino Children With Asthma
Authors: Fruchter Natalie , Arcoleo Kimberly , Rastogi Deepa , Serebrisky Denise , Warman Karen , Feldman Jonathan M 
Published: September 2023

Abstract

Objective
This study examined the associations between attention-deficit/hyperactivity disorder (ADHD) symptoms, underperception of respiratory compromise, and illness representations in Black and Latino children with asthma. We hypothesized that increased child-reported ADHD symptoms, as well as parent reports for their child, would be associated with underperception of respiratory compromise, and maladaptive asthma beliefs.

Methods
Two hundred ninety-six parent–child dyads were recruited from pediatric asthma and primary care clinics in the Bronx. Participants completed demographic questionnaires, the Conners-3 ADHD Index to measure ADHD symptoms, and the Asthma Illness Representation Scale to assess asthma beliefs. Perception of respiratory compromise was assessed by programmable electronic peak flow monitors that measured the child’s subjective estimates of peak expiratory flow (PEF) and actual PEF, with underperception as the primary measure.

Results
Child-reported ADHD symptoms were associated with greater underperception (β = .117, p = .049) of respiratory compromise. Parent-reported ADHD symptoms were associated with greater underperception (β = .129, p = .028) of respiratory compromise. Child-reported ADHD symptoms (β = −.188, p &lt; .001) were associated with more maladaptive asthma beliefs, F(1, 341) = 13.135. Parent-reported ADHD symptoms (β = −.203, p ≤ .001) were associated with more maladaptive asthma beliefs, F(1, 341) = 15.644.

Conclusions
ADHD symptoms were associated with a greater underperception of respiratory compromise and more maladaptive asthma beliefs. Deficits of attentional processes and/or hyperactivity levels might be contributing factors. We emphasize the need for psychoeducation and interventions that improve perception and health beliefs in children with comorbid ADHD and asthma.
Adverse Childhood Events and Chronic Pain in Adolescents: The Role of Sleep Disturbance
Authors: Roman-Juan Josep , Solé Ester , Sánchez-Rodríguez Elisabet , Castarlenas Elena , Jensen Mark P , Miró Jordi 
Published: September 2023

Abstract

Objectives
This study aimed to (1) examine the extent to which the association between exposure to adverse childhood events (ACEs) and having chronic pain in adolescents is explained by the association between exposure to ACEs and sleep disturbance and (2) explore the role of sleep disturbance in the association between exposure to ACEs and anxiety and depressive symptoms in adolescents with chronic pain.

Methods
Cross-sectional data from 469 adolescents aged 13–18 years old were drawn from an epidemiological study on pediatric chronic pain conducted in Catalonia (Spain). Participants provided self-reports of demographic characteristics, exposure to ACEs, pain characteristics, sleep disturbance, anxiety, and depressive symptoms. Mediation and moderation models were conducted.

Results
Sleep disturbance explained a significant amount of the variance in the association between exposure to ACEs and the presence of chronic pain. Moreover, sleep disturbance explained a significant amount of the variance in the association between exposure to ACEs and depressive symptoms and moderated the association between exposure to ACEs and anxiety in adolescents with chronic pain.

Conclusion
The study findings suggest the possibility that addressing sleep disturbance in adolescents exposed to ACEs may help to prevent the development of chronic pain, anxiety, and depressive symptoms in those adolescents who already have chronic pain.
Trimethoprim–Sulfamethoxazole for Pediatric Osteoarticular Infections
Authors: McDaniel Lauren M , Fiawoo Suiyini , Tamma Pranita D , Same Rebecca G 
Published: September 2023

Abstract

Background
Trimethoprim–sulfamethoxazole (TMP–SMX) is active against most Staphylococcus aureus isolates but is not widely used for the treatment of pediatric osteoarticular infections.

Methods
This was a comparative effectiveness study of hospitalized patients ≤18 years treated with TMP–SMX vs. other antibiotic regimens for acute osteoarticular infections between 2016 and 2021 at 3 hospitals using inverse probability of treatment weighted propensity score analysis. The primary outcome was treatment failure, a composite of unanticipated emergency department (ED) or outpatient visits, hospital readmissions, extension, or change of antibiotic therapy due to inadequate clinical response, or death, all within 6 months after completing antibiotics. The secondary outcome was antibiotic-associated adverse events (AEs) within 6 months. The exposed group for the treatment failure analysis included children who received ≥7 days of TMP–SMX and did not experience treatment failure while on another antibiotic. Children receiving at least 1 dose of TMP–SMX were the exposed group for the AE analysis.

Results
One-hundred and sixteen patients met eligibility criteria; 26 (22.4%) patients were classified into the TMP–SMX cohort and 90 (77.6%) into the other antibiotics cohort (most commonly clindamycin, vancomycin, and cefazolin). There was no significant difference in treatment failure between TMP–SMX and other antibiotics (43% vs. 19%; 95% CI .9–10.4). More patients in the TMP–SMX cohort experienced an unplanned ED or outpatient visit (OR 4.8, 95% CI 1.3–17.8). There was no difference in hospital readmission, antibiotic change, or duration extension. Exposure to TMP–SMX was associated with more AEs (41% vs. 19%, P = .012).

Conclusions
Treatment with TMP–SMX was not associated with greater clinical failure but was associated with more AEs compared to alternative agents for the treatment of pediatric acute osteoarticular infections.
Novel peptide calcitonin gene-related peptide antagonists for migraine therapy
Authors: Killoran Patrick M , Capel Vicky , D’Aloisio Vera , Schofield Adam , Aczél Tímea , Bölcskei Kata , Helyes Zsuzsanna , von Mentzer Bengt , Kendall David A , Coxon Chris R , Hutcheon Gillian A 
Published: September 2023

Abstract

Objectives
It has previously been shown that the peptide (34Pro,35Phe)CGRP27–37 is a potent calcitonin gene-related peptide, CGRP receptor antagonist, and in this project we aimed to improve the antagonist potency through the structural modification of truncated C-terminal CGRP peptides.

Methods
Six peptide analogues were synthesized and the anti-CGRP activity confirmed using both in vitro and in vivo studies.

Key findings
A 10 amino acid-containing peptide VPTDVGPFAF-NH2 (P006) was identified as a key candidate to take forward for in vivo evaluation, where it was shown to be an effective antagonist after intraperitoneal injection into mice. P006 was formulated as a preparation suitable for nasal administration by spray drying with chitosan to form mucoadhesive microcarriers (9.55 ± 0.91 mm diameter) and a loading of 0.2 mg peptide per 20 mg dose.

Conclusions
The project has demonstrated the potential of these novel small peptide CGRP antagonists, to undergo future preclinical evaluation as anti-migraine therapeutics.
Laparoscopic cholecystectomy: post-operative bile and chyle leaks. A case report
Authors: Philip Dwight , Garcia Maxine , Anika Maisha , Avila Azalia , Seaver Christopher 
Published: September 2023

Abstract
One week after an elective laparoscopic cholecystectomy at an outside hospital, a 56-year-old male presented to the emergency department with right-sided abdominal pain. Computerized tomography (CT) revealed a complex fluid collection in the gallbladder fossa. The patient underwent drain placement and received broad-spectrum intravenous antibiotics. Drain output was suspicious for a chyle leak, which was confirmed by elevated fluid triglyceride levels. Magnetic resonance cholangiopancreatography (MRCP) and hepatobiliary iminodiacetic acid (HIDA) analysis showed evidence of a concurrent bile leak. After starting a low fat, high protein diet and octreotide, a common bile duct sphincterotomy with plastic stent placement was performed. The patient’s symptoms and drain output proceeded to improve. The cause of the chyle leak is unclear. However, with consideration of the patient’s concurrent bile leak, an injury to the right major lymphatic drainage pathway and adjacent bile duct is suspected.
Massive stone or is it glass: a curious case of porcelain gallbladder
Authors: Kendric Tan Jun Guang , Wijesuriya Ruwan 
Published: September 2023

Abstract
Usage of computed tomography (CT) scans has increased exponentially over the past decade. This is associated with the rise in incidental findings and having to manage clinical scenarios previously never encountered in the pre-CT scan era. Once such finding is a porcelain gallbladder, specifically gallbladder wall calcification. We report one such case of a porcelain gallbladder mimic and propose some suggestions on the decision-making process when managing an incidentally discovered calcified gallbladder.
Diagnosis of invasive pulmonary aspergillosis by lateral flow assay of galactomannan in bronchoalveolar lavage fluid: a meta-analysis of diagnostic performance
Authors: Cai Yingli , Liang Jun , Lu Guangsheng , Zhan Yankun , Meng Jianwei , Liu Zhusheng , Shao Yiming 
Published: September 2023

Abstract
The performance of lateral flow assay (LFA) in diagnosing invasive pulmonary aspergillosis (IPA) has not been well demonstrated. To address this, we conducted a meta-analysis assessing the overall accuracy of LFA in diagnosing IPA using bronchoalveolar lavage fluid (BALF). Over a systematical search and assessment of bias risk, we calculated the pooled specificity, sensitivity, and area under the receiver operating curve (AUC) to assess the diagnostic performance. Our meta-analysis included 11 studies. The combined total sensitivity and specificity for diagnosing IPA were 0.78 (95% confidence interval (CI): 0.71, 0.83) and 0.87 (95% CI: 0.81, 0.91), respectively. The AUC was 0.86 (95% CI: 0.82, 0.89). Our results demonstrate that LFA using galactomannan in BALF exhibits high sensitivity and specificity for diagnosing IPA.
Schistosomiasis: The Disease That Saved Taiwan
Authors: Turner Matthew D , Sapp Jason 
Published: September 2023

ABSTRACT
From 1949 to 1950, an acute epidemic of schistosomiasis struck several elite units of the People’s Liberation Army during the Chinese Civil War that were preparing for an amphibious invasion of Taiwan. The crucial delay brought by the sudden outbreak of the disease may have cost communist forces control of the strategic island, changing the geopolitical calculus of the Pacific in the decades since.