bluuebunny/crossref_metadata_2025_split huggingface dataset:
Untargeted metabolomics of saliva in caries-active and caries-free children in the mixed dentition
Authors: Li Yueheng , Yang Zhengyan , Cai Ting , Jiang Dan , Luo Jun , Zhou Zhi 
Published: April 2023

ObjectiveTo compare the differences in salivary metabolites between caries-active and caries-free children in the mixed dentition, and explore their correlation with caries status.MethodsThe study involved 20 children (aged 8–9 years) in the mixed dentition, including 10 caries-active (aged 8.6 ± 0.49years) and 10 caries-free children(aged 8.5 ± 0.5years), with a male/female ratio of 1:1. The saliva samples were collected from all children. Metabolite extraction, LC-MS/MS-based untargeted metabolomics, qualitative and semi-quantitative analysis and bioinformatics analysis were performed to identify differential metabolites between the two sample groups. The differential metabolites identified were further analyzed in an attempt to find their correlations with caries status.ResultsIn the positive ion mode, a total of 1606 molecular features were detected in the samples of the two groups, 189 of which were differential metabolites when comparing the caries-active group with the caries-free group, including 104 up-regulated and 85 down-regulated metabolites. In the negative ion mode, a total of 532 molecular features were detected in the samples of two groups, 70 of which were differential metabolites when comparing the caries-active group with the caries-free group, including 37 up-regulated and 33 down-regulated metabolites. In the positive ion mode, two of the top 5 up-regulated differential metabolites were found in and annotated to specific metabolic pathways, whereas in the negative ion mode, only one of the top 5 up-regulated differential metabolites was found in and annotated to specific metabolic pathways. In both the positive and negative ion modes, the top 5 down-regulated differential metabolites were both annotated to the metabolic pathways. KEGG pathway enrichment analysis of differential metabolites showed that histamine and arachidonic acid identified in the positive ion mode, as well as succinate and L-histidine identified in the negative ion mode were enriched in the top 3 significantly altered pathways.ConclusionThe enriched differential metabolites including histamine, L-histidine and succinate were correlated with the presence of dental caries, but their role in the caries process needs to be further investigated.
Usefulness of direct intratumoral administration of doxorubicin hydrochloride with an electro-osmosis–assisted pump
Authors: Ito Ayu , Itakura Shoko , Hasegawa Yuya , Hashimoto Miyu , Okada Akie , Hirafuji Mamoru , Nakamura Hidenori , Sugibayashi Kenji , Todo Hiroaki 
Published: April 2023

Patients receiving chemotherapy by intravenous (i.v.) or oral administration of anticancer drugs often experience side effects. In this study, an electro-osmotic flow (EO) pump was used for the direct administration of an anticancer drug with minimum side effects. Doxorubicin hydrochloride (DXR) was used as an anticancer drug, and its antitumor effect and toxicity were evaluated in comparison with i.v. administration. Balb/c female mice were subcutaneously transplanted with a breast cancer cell line (4T1/Luc) stably expressing luciferase, and 20 μL of DXR solution (1.0 mg/mL) was administered intratumorally (i.t.) at a slow rate (0.6 µL/min) using an EO pump or rapidly using a syringe. For comparison, 100 μL of DXR solution was injected through the tail vein at the same dose and a 5-times higher dose. A tumor growth inhibitory effect without significant weight loss was observed with direct i.t. administration of DXR using an EO pump. On the other hand, no suppressive tumor growth effect was observed with i.v. administration of DXR at the same dose. Although there was no significant difference in the suppression effect on tumor growth between i.t. administration with EO pump and syringe, the peripheral skin concentration of DXR were decreased after slow administration with EO pump compared with that after rapidly administration with a syringe. These results indicated that direct i.t. administration of DXR with lower dosing using an EO pump at slower administration rate may be useful for exhibiting antitumor effects and suppressing systemic side effects. In addition, the blood concentration and the peripheral skin concentration of DXR after administration at lower rate with EO pump were decreased compared with those after the rapidly administration with a syringe.
Development of anti-foulant ultraviolet-assisted polyvinyl alcohol layer on the polysulfone-based nanohybrid membrane for industrial rubber wastewater decontamination
Authors: Kusworo Tutuk Djoko , Kumoro Andri Cahyo , Aryanti Nita , Lingga Fadhilah Fatma , Widiastuti Ade , Vetcher Alexandre A. , Dalanta Febio 
Published: April 2023

Introduction: Membrane fouling has been reported to be one of the bottlenecks of membrane technologies for wastewater treatment. To mitigate its negative impacts, we fabricated polysulfone membrane (PSf) composites made of silica (SiO2) and graphene oxide (GO) nanoparticles that modified with ultraviolet (UV)-assisted polyvinyl alcohol layer on the membrane surface.Methods: The membrane composite was synthesized using non-solvent induced phase separation (NIPS) method. The membrane was further treated by UV irradiation and cross-linked with PVA coating to cope with the fouling problem. The modified membrane was applied for industrial rubber wastewater decontamination.Results: The UV irradiation and cross-linked PVA coating to the PSf/GO-SiO2 membrane improved the pseudo-steady state permeate flux by 60.15% from 20.05 to 50.32 L/m2hr and maintained the permeate flux up to 82.33%. About 85% of total dissolved solids (TDS), 81% of chemical oxygen demand (COD), and 84% of ammonia compound (NH3) with initial concentrations of 335.76, 242.55, 175.19 mg/L, respectively, could be removed after 8 h of membrane treatment. The modified membrane also exhibited an excellent flux recovery ratio of up to 83%.Discussion: The modified membrane changed the fouling mechanism from pore blockage to cake filtration, which signifies the capability of the membrane to tackle severe fouling tendency. The cross-linked UV/PVA coating reduced fouling formation by reducing the adsorptive interactions between the foulant molecules and the membrane surface by enhancing membrane surface hydrophilicity. This implies that incorporating GO/SiO2 nanoparticles with UV irradiation and PVA coating substantially enhanced the physicochemical properties of the PSf membrane.
Concern about the human health implications of marine biodiversity loss is higher among less educated and poorer citizens: Results from a 14-country study in Europe
Authors: Davison Sophie M. C. , White Mathew P. , Pahl Sabine , Taylor Tim , Borja Angel , McMeel Oonagh , Kellett Paula , Roberts Bethany R. , Fleming Lora E. 
Published: April 2023

IntroductionMarine biodiversity loss has direct and indirect effects on human health and wellbeing. Recent European data suggest that the public is aware of this, identifying marine biodiversity protection as its top research priority in terms of oceans and human health, rated higher than issues such as plastic, chemical, and microbial pollution.MethodsThe current study aimed to better understand key sociodemographic and personality predictors of concern about marine biodiversity loss and the desire for more research into marine biodiversity protection, in an attempt to support communication efforts targeting specific sectors in society. Data were drawn from nationally representative samples of 14 European countries (n = 14,167).ResultsResults show greater concern about marine biodiversity loss and support for more research into marine biodiversity protection by older adults, females, and individuals: (i) without (vs. with) a university degree; (ii) with lower (vs. middle) incomes; (iii) who identified as politically left-wing; (iv) who visited the coast more often; and (v) those with more open, agreeable and conscientious personalities.DiscussionThese results suggest that, although concern and research support are generally high among European citizens, policy makers and communicators need to take into consideration individual-level variation.
Effects of awake prone position vs. usual care on acute hypoxemic respiratory failure in patients with COVID-19: A systematic review and meta-analysis of randomized controlled trials
Authors: Peng Qing , Yang Sheng , Zhang Yu , Zhao Wenjie , Hu Man , Meng Bo , Ni Huanhuan , Min Lingfeng , Yu Jiangquan , Wang Yongxiang , Zhang Liang 
Published: April 2023

BackgroundPrevious studies have shown that an awake prone position may be beneficial for the treatment of acute respiratory distress syndrome (ARDS) or acute hypoxic respiratory failure (AHRF) in patients with COVID-19, but the results are not consistent, especially in terms of oxygenation outcomes and intubation rate. This systematic review and meta-analysis assessed the effects of the awake prone position on AHRF in patients with COVID-19 with all randomized controlled trials (RCTs).MethodsAn extensive search of online databases, including MEDLINE, Embase, Web of Science, and Cochrane Central Register of Controlled Trials from 1 December 2019 to 30 October 2022, with no language restrictions was performed. This systematic review and meta-analysis are based on the PRISMA statement. We only included RCTs and used the Cochrane risk assessment tool for quality assessment.ResultsFourteen RCTs fulfilled the selection criteria, and 3,290 patients were included. A meta-analysis found that patients in the awake prone position group had more significant improvement in the SpO2/FiO2 ratio [mean difference (MD): 29.76; 95% confidence interval (CI): 1.39–48.13; P = 0.001] compared with the usual care. The prone position also reduced the need for intubation [odd ratio (OR): 0.72; 95% CI: 0.61 to 0.84; P &lt; 0.0001; I2 = 0%]. There was no significant difference in mortality, hospital length of stay, incidence of intensive care unit (ICU) admission, and adverse events between the two groups.ConclusionThe awake prone position was a promising intervention method, which is beneficial to improve the oxygenation of patients with ARDS or AHRF caused by COVID-19 and reduce the need for intubation. However, the awake prone position showed no obvious advantage in mortality, hospital length of stay, incidence of ICU admission, and adverse events.Systematic review registrationInternational Prospective Register of Systematic Reviews (PROSPERO), identifier: CRD42022367885.
Meta-analysis of the association between dietary inflammatory index and cognitive health
Authors: Ding Tianze , Aimaiti Maimaitiyusupu , Cui Shishuang , Shen Junhao , Lu Mengjie , Wang Lei , Bian Dongsheng 
Published: April 2023

BackgroundSome studies have shown that a pro-inflammatory diet may be associated with cognitive function, but their conclusions have varied considerably. We here present a meta-analysis of the current published literature on DII score and its association with cognitive health.MethodsIn this meta-analysis, the PubMed, Embase, Web of Science, and Cochrane databases were searched in September 2022. The reported indexes, specifically OR, RR, and β, were extracted and analyzed using R version 3.1.0.ResultsA total of 636 studies in databases were identified, and 12 were included in the meta-analysis. Higher DII was associated with an increased risk of AD and MCI (OR = 1.34; 95% CI = 1.21–1.49). Meanwhile, it may also cause global function impairment (categorical: OR = 1.63; 95% CI = 1.36–1.96) and verbal fluency impairment (continuous: OR = 0.18; 95% IC = 0.08–0.42). But there was no significant association between DII and executive function (categorical: OR = 1.12; 95% IC = 0.84–1.49; continuous: OR = 0.48; 95% IC = 0.19–1.21) or episodic memory (continuous: OR = 0.56; 95% IC = 0.30–1.03).ConclusionA pro-inflammatory diet is related to AD, MCI, and the functions of some cognitive domains (specifically global function and verbal fluency). However, the current evidence on the role of diet-induced inflammation in different cognitive domains should be supported by further studies in the future.
Predictive power of deep-learning segmentation based prognostication model in non-small cell lung cancer
Authors: Gainey Jordan C. , He Yusen , Zhu Robert , Baek Stephen S. , Wu Xiaodong , Buatti John M. , Allen Bryan G. , Smith Brian J. , Kim Yusung 
Published: April 2023

PurposeThe study aims to create a model to predict survival outcomes for non-small cell lung cancer (NSCLC) after treatment with stereotactic body radiotherapy (SBRT) using deep-learning segmentation based prognostication (DESEP).MethodsThe DESEP model was trained using imaging from 108 patients with NSCLC with various clinical stages and treatment histories. The model generated predictions based on unsupervised features learned by a deep-segmentation network from computed tomography imaging to categorize patients into high and low risk groups for overall survival (DESEP-predicted-OS), disease specific survival (DESEP-predicted-DSS), and local progression free survival (DESEP-predicted-LPFS). Serial assessments were also performed using auto-segmentation based volumetric RECISTv1.1 and computer-based unidimensional RECISTv1.1 patients was performed.ResultsThere was a concordance between the DESEP-predicted-LPFS risk category and manually calculated RECISTv1.1 (φ=0.544, p=0.001). Neither the auto-segmentation based volumetric RECISTv1.1 nor the computer-based unidimensional RECISTv1.1 correlated with manual RECISTv1.1 (p=0.081 and p=0.144, respectively). While manual RECISTv1.1 correlated with LPFS (HR=6.97,3.51-13.85, c=0.70, p&lt;0.001), it could not provide insight regarding DSS (p=0.942) or OS (p=0.662). In contrast, the DESEP-predicted methods were predictive of LPFS (HR=3.58, 1.66-7.18, c=0.60, p&lt;0.001), OS (HR=6.31, 3.65-10.93, c=0.71, p&lt;0.001) and DSS (HR=9.25, 4.50-19.02, c=0.69, p&lt;0.001). The promising results of the DESEP model were reproduced for the independent, external datasets of Stanford University, classifying survival and ‘dead’ group in their Kaplan-Meyer curves (p = 0.019).ConclusionDeep-learning segmentation based prognostication can predict LPFS as well as OS, and DSS after SBRT for NSCLC. It can be used in conjunction with current standard of care, manual RECISTv1.1 to provide additional insights regarding DSS and OS in NSCLC patients receiving SBRT.SummaryWhile current standard of care, manual RECISTv1.1 correlated with local progression free survival (LPFS) (HR=6.97,3.51-13.85, c=0.70, p&lt;0.001), it could not provide insight regarding disease specific survival (DSS) (p=0.942) or overall survival (OS) (p=0.662). In contrast, the deep-learning segmentation based prognostication (DESEP)-predicted methods were predictive of LPFS (HR=3.58, 1.66-7.18, c=0.60, p&lt;0.001), OS (HR=6.31, 3.65-10.93, c=0.71, p&lt;0.001) and DSS (HR=9.25, 4.50-19.02, c=0.69, p&lt;0.001). DESEP can be used in conjunction with current standard of care, manual RECISTv1.1 to provide additional insights regarding DSS and OS in NSCLC patients.
Normalized difference vegetation index sensor-based nitrogen management in bread wheat (Triticum aestivum L.): Nutrient uptake, use efficiency, and partial nutrient balance
Authors: Mitra Biplab , Singha Prantick , Roy Chowdhury Arnab , Sinha Abhas Kumar , Skalicky Milan , Brestic Marian , Alamri Saud , Hossain Akbar 
Published: April 2023

The present experiment was conducted to assess the impact of fixed and variable doses (using a normalized difference vegetation index-sensor) of nitrogen (N) on wheat yields, nutrient uptake, nitrogen use efficiency, and soil nitrogen balance through the optimization of nitrogen dose. There were 10 treatments based on fixed and variable doses with different splits, and each treatment was replicated three times under a randomized complete block design. The treatments comprised fixed doses of 120 and 150 kg N ha–1with different splits; variable doses based on sensor readings after application of 60, 90, and 120 kg N ha–1; 225 kg N ha–1as a nitrogen-rich control; and no application of nitrogen as the absolute control. It was revealed that the application of a basal dose of 60 kg N ha–1and another 60 kg N ha–1at the crown root initiation stage followed by a sensor-guided N application significantly improved wheat grain yields and grain nitrogen uptake. However, straw nitrogen uptake was highest in N-rich plots where 225 kg N ha–1was applied. It was found that any curtailment in these doses at basal and crown root initiation stages followed by nitrogen application using a normalized difference vegetation index sensor later could not bring about higher crop yields. On average, wheat crops responded to 152–155 kg N ha–1in both years of the study. Partial factor productivity along with agronomic and economic nitrogen use efficiency showed a declining trend with an increased rate of N application. Apparent N recovery values were comparable between normalized difference vegetation index sensor-based N application treatments and treatments receiving lesser N doses. Soil N status decreased in all the treatments except the nitrogen-rich strip, where there was a marginal increase in soil N status after the wheat crop harvest in the rotation. Partial nitrogen balance was negative for all the treatments except the control. From these 2-year field trials, it can be concluded that applying a normalized difference vegetation index sensor could be an essential tool for the rational management of fertilizer nitrogen in wheat grown in eastern sub-Himalayan plains.
The Experiences Surrounding Romantic Relationships of Korean Bisexual Individuals
Authors: Park Jeongeun , Chung Seojin 
Published: April 2023

IntroductionIn recent years, the level of social and academic interest in sexual minorities has been gradually increasing. However, studies on bisexual individuals and the data on experiences of bisexual individuals outside the United States are scarce.MethodsTo decrease the gap in the literature, this study examined the experiences of South Korean bisexual individuals in the context of romantic relationships via semi-structured, in-depth interviews. Participants were recruited through online-based platforms, and the interview with eight participants was analyzed.ResultsFive main themes were identified: discovering myself as bisexual, being exposed to discrimination and exclusion, being affected by the sex of a romantic partner, protecting myself when engaging in a romantic relationship, and benefits of a romantic relationship.DiscussionThe findings suggest that Korea’s heteronormative system and culture were the major challenges perceived by the participants. In conclusion, this study illustrates how sociocultural norms, social acceptance, and recognition affect bisexual identity in a romantic relationship.
The health benefits of bicycling to school among adolescents in China: A propensity score matching study
Authors: Ding Pengxiang , Ding Chang , Feng Suwei 
Published: April 2023

BackgroundA large number of high-income countries are now promoting active commuting to school as an opportunity for adolescents to increase physical activity (PA) and improve their health. Few studies have examined the multiple benefits of active travel to school among adolescents in developing countries, especially in China. Hence, this study aims to estimate the effects of bicycling to school on adolescents’ subjective health, physical health, and sickness absence.MethodsSelf-reported and cross-sectional data from 6,353 school-aged children (12–19 years old) in the 2014–2015 China Education Panel Survey (CEPS2014-2015) were used. The independent variable was a binary, self-reported indicator of whether children bicycled to school. The dependent variables included subjective health (self-reported health, mental stress), physical health (BMI, kidney disease, lung disease, heart disease, brain disease, upper limb fracture, lower limb fracture, and sickness frequency), and sickness absence. Propensity score matching (PSM) was used to estimate the causal effects of bicycling to school on adolescents’ health.ResultsBicycling to school positively affects both subjective and physical health. Those students who biked to school were associated with a higher self-rated health status, a healthier weight, a lower level of mental stress, and a lower risk of developing brain diseases. No significant relation is found between bicycling and sickness frequency, and sickness absence. Moreover, we separately compared the bicycling group with the walking group and the non-active travel group. There is still evidence that cycling is beneficial for students. Compared with walking to school, cycling to school resulted in a higher self-rated health score and a lower mental stress score. Physically, students who bicycled to school were less likely to be absent from school and suffer from kidney and brain diseases than students who walked to school. However, we do not find a significant difference in health outcomes from cycling compared to non-active modes of transportation. Further, differentiation of the health effects of bicycling across living areas shows that health effects are more pronounced for those living in edge and rural areas.ConclusionThese findings provide evidence of the value of promoting bicycling to school in improving various adolescents’ health outcomes in transitional China.
Age- and sex-specific associations between sarcopenia severity and poor cognitive function among community-dwelling older adults in Japan: The IRIDE Cohort Study
Authors: Ohta Takahisa , Sasai Hiroyuki , Osuka Yosuke , Kojima Narumi , Abe Takumi , Yamashita Mari , Obuchi Shuichi P. , Ishizaki Tatsuro , Fujiwara Yoshinori , Awata Shuichi , Toba Kenji ,   IRIDE Cohort Study Investigators
Published: April 2023

IntroductionThis study examined whether the association between sarcopenia severity and cognitive function differed according to sex and age in community-dwelling older adults in Japan.MethodsThis is a cross-sectional study of older adults (age ≥ 65 years) consisting of five regional cohorts integrated as the Integrated Research Initiative for Living Well with Dementia (IRIDE) Cohort Study. Sarcopenia severity was determined based on the Asian Working Group for Sarcopenia 2019, which assessed grip strength, walking speed, and skeletal muscle mass index. Poor cognitive function was defined as a Mini-Mental State Examination score of ≤ 23. Odds ratios (ORs) and 95% confidence intervals (CIs) for poor cognitive function were calculated by sex and age group (65–74 and ≥75 years) using binomial logistic regression models, which were adjusted for age, educational attainment, history of non-communicable diseases, smoking and drinking habits, living alone, frequency of going outdoors, exercise habits, and depressive symptom.ResultsOf the 8,180 participants, 6,426 (1,157 men aged 65–74 and 1,063 men aged 75 or older; 2,281 women aged 65–74 and 1,925 women aged 75 or older) were analyzed. The prevalence ratio of sarcopenia and severe sarcopenia were 309 (13.9%) and 92 (4.1%) among men and 559 (13.3%) and 166 (3.7%) among women, respectively. A total of 127 (5.8%) men and 161 (3.9%) women had a poor cognitive function. Setting non-sarcopenia as a reference, the adjusted ORs (95% CI) of poor cognitive function were 2.20 (1.54, 3.15) for sarcopenia and 3.56 (2.20, 5.71) for severe sarcopenia. A similar trend was observed in analyses stratified by sex and age, with linear associations (P for trend &lt;0.05) in both categories. Furthermore, there was a significant interaction (P &lt; 0.05) between sex and sarcopenia severity, indicating a stronger linear association of sarcopenia severity with poor cognitive function in women compared with men.Discussion and conclusionSarcopenia severity was linearly associated with poor cognitive function in adults aged ≥ 65 years, with a stronger association in women compared with men.