bluuebunny/crossref_metadata_2025_split huggingface dataset:
A correlational study of Uranium in groundwater with other physico- chemical parameters using GIS mapping in Godda district, Jharkhand
Authors: Srivast Mansi , Srivas P. K. 
Published: March 2023

Abstract

The present research concentrates on the cumulative use of GPS and GIS technologies, which are excellent resources for analyzing and monitoring divergent physico-chemical parameters in groundwater, including pH, TDS, E.C., ORP, Ca+2, Mg+2, NO3-, F-, SO4-2, Cl-, and PO4-3 with explicit regard to Uranium. Garmin GPS is used to record the locations of the sampling points in the Godda study area. The research aims to offer a thorough understanding of the relationship between soil and water, its impact on public health and the extent to which water can be used in various ways based on its quality. Utilizing the Inverse Distance Weighted (IDW) technique, it is examined how these groundwater parameters and the Water Quality Index can be estimated spatially. Additionally, a correlation analysis of the water quality parameters is computed to estimate the local population's cancer risk living in the study area. Except for calcium and magnesium, which are present in excess concentrations throughout the study area with the highest values of 325 and 406 mg/l, respectively, at Amediha, Meherma, the results showed that the maximum concentration parameters are within limits with the standard. The main reason might be the area's predominance of alfisols soil type. The radioactive element uranium is found to be in a limited number. Chemo-toxicity and radiological risk assessment of the whole area lie far below the restricted cancer risk limit, i.e. 30 ppb with the highest concentration of 14 ppb in the "Sunderpahari" region, following the results obtained. The water quality index for the area ranges from "Good" to "Very Poor." The results were favorable, but a few sites, such as 'Boarijor' and its surroundings, require additional attention to enhance groundwater quality. Given Uranium's low availability in groundwater, the region's cancer risk assessment is below average.
Estimation of Transverse Velocity and Concentration Profile Using Kumaraswamy Distribution
Authors: Sharma Himanshu , Joshi N. 
Published: March 2023

Abstract
To measure the lateral distribution of the velocity is very important as it is associated with riverine flow processes like distribution of shear stress, sediment concentration distribution, diffusion coefficients, secondary current’s distribution, etc. Many formulations were proposed for lateral distribution of velocity with each being somehow complex in its application. In present study Kumaraswamy’s distribution was utilized to propose a lateral velocity distribution which uses mean velocity and Kumaraswamy’s parameters a and b. It was observed in the study that skewness in the velocity distribution is a function of both a and b. Kumaraswamy’s distribution have been observed to predict the velocity profile in a satisfactory manner and when utilized to predict the transverse concentration profile along-with Ahmad’s scheme, predicted it satisfactorily.
Compact surfaces with boundary with prescribed mean curvature depending on the Gauss map
Authors: Bueno Antonio , López Rafael 
Published: March 2023

Abstract
Given a C1 function H defined in the unit sphere S2, an H-surface M is a surface in the Euclidean space R3 whose mean curvature HM satisfies HM(p) = H(Np), p ∈ M, where N is the Gauss map of M. Given a closed simple curve Γ ⊂ R3 and a function H, in this paper we investigate the geometry of compact H-surfaces spanning Γ in terms of Γ. Under mild assumptions on H, we prove non-existence of closed H-surfaces, in contrast with the classical case of constant mean curvature. We give conditions on H that ensure that if Γ is a circle, then M is a rotational surface. We also establish the existence of estimates of the area of H-surfaces in terms of the height of the surface.
Mathematics Subject Classification: 53A10, 53C42, 35J93, 35B06, 35B50.
Online citizen science with the Zooniverse for analysis of biological volumetric data
Authors: Smith Patricia , King Oliver NF , Pennington Avery , Tun Win , Basham Mark , Jones Martin L , Collinson Lucy M , Darrow Michele C , Spiers Helen 
Published: March 2023

Abstract
Public participation in research, also known as citizen science, is being increasingly adopted for the analysis of biological volumetric data. Researchers working in this domain are applying online citizen science as a scalable distributed data analysis approach, with recent research demonstrating that non-experts can productively contribute to tasks such as the segmentation of organelles in volume electron microscopy data. This, alongside the growing challenge to rapidly process the large amounts of biological volumetric data now routinely produced, means there is increasing interest within the research community to apply online citizen science for the analysis of data in this context. Here, we synthesise core methodological principles and practices for applying citizen science for analysis of biological volumetric data. We collate and share the knowledge and experience of multiple research teams who have applied online citizen science for the analysis of volumetric biological data using the Zooniverse platform (www.zooniverse.org). We hope this provides inspiration and practical guidance regarding how contributor effort via online citizen science may be usefully applied in this domain.
Malaria misdiagnosis in the routine health system in Arba Minch area district in southwest Ethiopia: an implication for malaria control and elimination
Authors: Yigezu Engida , Wondale Biniam , Abebe Daniel , Tamiru Girum , Eligo Nigatu , Lindtjørn Bernt , Gadisa Endalamaw , Tadesse Fitsum Girma , Massebo Fekadu 
Published: March 2023

Abstract
Background
Microscopy and Rapid Diagnostic Test (RDT) are the two widely used malaria diagnostic tools in Ethiopian health facilities. Accurate diagnosis is vital for appropriate treatment of cases to interrupt its transmission. Missing malaria cases, species misdiagnosis and false positive or negative reports might compromise the control and elimination effort. Therefore, this study assessed the status of species-specific misdiagnosis by microscope compared with polymerase chain reaction (PCR).
Methods
A cross-sectional survey based on health facilities was conducted from November 2019 to January 2020.
Results
A total of 254 microscopically negative and 193 microscopically positive malaria cases were included. Of the 193 malaria positive cases, 46.1% (95% confidence interval (CI): 38.9–53.4) (89/193) were Plasmodium falciparum infection, 52.3% (95% CI: 45.0-59.5) (101/193) were P. vivax infection, and 1.6% (3/193) had mixed infection of P. falciparum and P. vivax. Of the microscopically positive cases of P. falciparum, 84.3% (75/89) were confirmed as P. falciparum, 3.4% (3/89) were P. vivax and 11.2% (10/89) were mixed infections with P. falciparum and P. vivax and only one case was negative molecularly. Similarly, of the microscopically positive cases of P. vivax cases, 92.1% (93/101) were confirmed as P. vivax, 5.9% (6/101) were P. falciparum and 1% (1/101) was mixed infection. Single case was negative by molecular technique. Of the 254 microscopically negative cases, 0.8% of cases were tested positive for P. falciparum and 2% for P. vivax. Considering molecular technique as a reference, the sensitivity of microscopy for detecting P. falciparum was 89.2 and for P. vivax, it was 91.2. The specificity of microscopy for detecting P. falciparum was 96.1 and for P. vivax, it was 97.7. However, the sensitivity of microscopy in detecting mixed infection of P. falciparum and P. vivax was low (8.3%).
Conclusion
Based on our results, many P. falciparum and P. vivax mixed infections were microscopically overlooked and underreported in the routine healthcare system. In addition, there were cases left untreated or inappropriately treated. Therefore, to minimise this problem, the gaps in the microscopic-based malaria diagnosis should be identified and regularly monitored to ensure the accuracy of the diagnosis.
Tricuspid Annular Area and Leaflets Stretch are Associated with Functional Tricuspid Regurgitation – Insights from Three-Dimensional Transesophageal Echocardiography –
Authors: Ogawa Mana , Ito Asahiro , Ito Ayaka , Kim Andrew T , Ishikawa Sera , Iwata Shinichi , Takahashi Yosuke , Izumiya Yasuhiro , Shibata Toshihiko , Fukuda Daiju 
Published: March 2023

Abstract
Background
The presence of functional tricuspid regurgitation (TR) is associated with mortality and morbidity. Although uniform management with a tricuspid annuloplasty ring is currently considered as a standard surgical procedure, high rates of residual TR despite annuloplasty are reported. Therefore, the identification of the TR mechanisms would be necessary to provide personalized treatment for each TR patient.
Methods
This study population consisted of 106 patients with mitral regurgitation (MR) who were scheduled for procedure. Transthoracic and transesophageal echocardiography were performed prior to mitral valve intervention. We performed three-dimensional quantitative assessment including tricuspid annular (TA) area and the distance between the three commissures of tricuspid valve.
Results
Significant TR, which is defined as moderate or greater TR, was detected in 23 (22%). TA area (P < 0.01), the distance of septal-leaflet length (SL) (P = 0.03) and posterior-leaflet length (PL) (p = 0.02) were significantly associated with significant TR, while TA diameter assessed by transthoracic echocardiography was not. When patients were divided into four groups according to SL and PL, the group with longer SL and PL had a significantly higher incidence of significant TR (P < 0.01).
Conclusions
Greater stretch of the septal and posterior leaflet between commissures and larger TA area are associated with significant TR in patients with severe MR. In order to prevent TR recurrence, the intervention of the septal leaflet in tricuspid annuloplasty may be beneficial. The precise implement of three-dimensional transesophageal echocardiography of tricuspid valve is valuable for a personalized strategy of tricuspid annuloplasty.
Numerical Modelling of Elastic Waves based on the Asymmetric Wave Equations
Authors: BAI WENLEI , LIU HONG , LI YOUMING , WANG ZHIYANG 
Published: March 2023

Abstract
It is a trend in the development of geophysics to seek wave theory closer to the physical reality and derive corresponding wave equations to achieve the high accurate forward modelling, imaging, and inversion of complex structures. The generalized continuum mechanics (GCM) theory enriches the context of the conventional continuum mechanics theory by introducing the additional characteristic length scale parameters to represent the microstructural properties of the medium, and the asymmetric elastic wave equations derived from GCM theory can handle the influence of heterogeneity of medium caused by the microstructure interactions on propagation of seismic waves. Up to the present stage, there are few researches on the numerical and analytical solutions of the elastic wave equations derived from the GCM theory, especially in the frequency band of seismic exploration. In addition, there are few studies in the existing literature that incorporate multiple theories and methods of the GCM theory into an integrated frame. In this paper, we introduce the concept of the multi-scale microstructure interactions and construct quantitative relationship between the characteristic length scale parameter and the characteristic length scale parameter of the micropore reflects the micropore structures, and then integrate the modified couple stress theory and the one-parameter second strain gradient theory into the unified framework for numerical modelling and analysis.
ARF6 promotes hepatocellular carcinoma proliferation through activating STAT3 signaling
Authors: Hu Yabing , Huang Yongchu , Xie Xiaohang , Li Longshan , Zhang Yong , Zhang Xiaochao 
Published: March 2023

Abstract
Purpose Hepatocellular Carcinoma (HCC) is one of the most common causes of cancer death worldwide. However, the molecular mechanism underlying HCC proliferation remains obscure. In this study, we show that high expression of ARF6 is related to a poor clinical prognosis, which could drive the proliferation of HCC.
Methods The expression level of ARF6 in HCC tissues was detected by immunohistochemistry and western blotting. Clinical significance of ARF6 was estimated in primary HCC patients. The effect of ARF6 on tumor proliferation was investigated by in vitro CCK8 and colony formation assay, and in vivo nude mouse xenograft models. ARF6 localization was investigated by immunofluorescence. Cell cycle-related proteins were evaluated by western blotting. Moreover, the correlation between ARF6 and STAT3 signaling in HCC was detected by western blotting, immunohistochemistry and xenograft assay.
Results ARF6 was overexpressed in HCC tissues compared to adjacent normal liver tissues and high expression of ARF6 was significantly correlated with poor tumor differentiation, incomplete tumor encapsulation, advanced tumor TNM stage and poor prognosis. ARF6 positively regulated the cell proliferation, colony formation, and cell cycle progression of HCC cells. In vivo nude mouse xenograft models proved the oncogenic role of ARF6 in tumor growth. In addition, ARF6 promoted the activation of STAT3 signaling and ARF6 expression was positively correlated with phosphorylated STAT3 in HCC tissues. Furthermore, interference of STAT3 demonstrated STAT3 as an important signaling pathway for the oncogenic role of ARF6 in HCC.
Conclusions Our findings indicate that ARF6 promotes HCC proliferation through activating STAT3 signaling, suggesting that ARF6 may serve as a novel prognostic biomarker and a therapeutic target for HCC patients.
Arsenic Sulfide EnhancesRadiosensitization in Rhabdomyosarcoma via Activating NFATc3-RAG1 Mediated DNA Double Strand Break (DSB)
Authors: Cai Yu , Zhu Chuanying , Lu Shumin , Kang Ting , Feng Zhuowei , Chen Shuxian , Chen Siyu 
Published: March 2023

Abstract
Background: Due to the high level of recurrence and metastasis, rhabdomyosarcoma (RMS) represents one of the most lethal soft-tissue sarcomas in children. It is thus imperative to explore a novel radiosensitizer to enhance the curative of radiotherapy in RMS patients. The trace element arsenic has been reported to function as a radiosensitizer in sarcomas. To determine whether arsenic sulfide (As4S4) potentiates radiation sensitization in RMS, we carried out this study to investigate the mechanism of As4S4 in radiotherapy.
Methods: RMS cell line (A-673) was treated with As4S4 and radiotherapy. Cell viability and drug-induced apoptosis were detected by cell counting kit-8 (CCK-8) and flow cytometry, respectively. Western blot and real-time qPCR were carried out to detect the nuclear factor of activated T-cells 3 (NFATc3) and recombination activating 1 (RAG1). DNA damage-associated proteins were also determined. For in vivo experiments, the therapeutic efficacy of As4S4-induced radiosensitization was evaluated via xenograft tumors in mice. To identify NFATc3 and RAG1, which were mostly involved in the mechanism of radiosensitization, we established a clinical cohort of 59 RMS patients. Immunohistochemistry (IHC) staining was applied to detect the expression of NFATc3 and RAG1 in RMS tissues in order to analyze the relationship with prognosis. We further developed a prediction model using stepwise logistic regression.
Results: As4S4 combined with radiotherapy exhibited predominant inhibition in RMS cells through CCK-8 and flow cytometry. We revealed that As4S4 as well as the knockdown of NFATc3 resulted in DSB in RMS cells by the increased expression of RAG1. Our in vivo experiment confirmed that co-treatment exerted efficient inhibition of RMS growth. In a clinical cohort of 59 RMS patients, survival analysis showed that NFATc3 and RAG1 were related to overall survival (OS). Cox regression analysis further indicated that NFATc3, RAG1, and Risk level could be regarded as independent prognostic factors for RMS patients.
Conclusions: In summary, As4S4 enhances radiosensitization in RMS via activating NFATc3-RAG1 mediated DNA DSB. NFATc3 and RAG1 are potential therapeutic targets in treating RMS. Our findings led us to conclude that As4S4 could be considered a radio-sensitizing agent for treating RMS.
High-Intensity Interval Training and Intake nano-selenium supplementation on the gene expression of hepatic SOD and CAT in Dexamethasone Induced Rats
Authors: ghane Motahare , Malayeri Shahin Riyahi , Hoseini Masume 
Published: March 2023

Abstract
Dexamethasone is one of the glucocorticoids which leads to oxidative damage. Therefore, antioxidants can reduce tissue toxicity to prevent the increase in oxidative stress caused by dexamethasone. The present study examines the effect of high-intensity interval training (HIIT) and nano-selenium supplementation on the expression of hepatic superoxide dismutase (SOD) and catalase (CAT) genes in dexamethasone-induced rats.
The samples were 40 Wistar rats in the age range of 8 weeks, which were randomly divided into 5 groups of 8 (healthy, induced by dexamethasone, dexamethasone+HIIT, dexamethasone+nano-selenium supplementation, dexamethasone+HIIT+nano-selenium supplementation). The exercise program was performed six days a week for four weeks. The prepared stock solution of selenium nanoparticles was given to mice in the amount of 100 mg by gavage every other day.
A two-way analysis of covariance was used to determine the difference in variables between groups at a significant level (P<0.05). The results showed that there was a significant increase in the expression of the hepatic SOD gene in the HIIT group with nano-selenium supplementation (P=0.034) compared to the control group. Also, hepatic CAT gene expression in each of the nano-selenium supplementation (P=0.002) and HIIT (P=0.010) groups was associated with a significant increase compared to the control group.
Therefore, it seems that the intervention of HIIT with the use of nano-selenium supplements will lead to the strengthening of the antioxidant system and the improvement of the immune system, and reduce the adverse effects caused by the use of steroid drugs such as dexamethasone.
The Interplay between Noncoding RNAs and p21 Signaling in Gastrointestinal
Cancer: From Tumorigenesis to Metastasis
Authors: Rahmani Farzad , Zandigohar Mehrdad , Safavi Pegah , Behzadi Maryam , Ghorbani Zeynab , Payazdan Mahya , Ferns Gordon , Hassanian Seyed Mahdi , Avan Amir 
Published: March 2023

Abstract:
Non-coding RNAs (ncRNAs) are emerging as important regulators in various pathological conditions,
including human cancers. NcRNAs exert potentially crucial effects on cell cycle progression, proliferation,
and invasion in cancer cells by targeting various cell cycle-related proteins at transcriptional and post-transcriptional
levels. As one of the key cell cycle regulatory proteins, p21 is involved in various processes, including
the cellular response to DNA damage, cell growth, invasion, metastasis, apoptosis, and senescence. P21 has
been shown to have either a tumor-suppressive or oncogenic effect depending on the cellular localization and
posttranslational modifications. P21 exerts a significant regulatory effect on both G1/S and G2/M checkpoints
by regulating the function of cyclin-dependent kinase enzymes (CDKs) or interacting with proliferating cell nuclear
antigen (PCNA). P21 has an important effect on the cellular response to DNA damage by separating DNA
replication enzymes from PCNA and inhibiting DNA synthesis resulting in G1 phase arrest. Furthermore, p21
has been shown to negatively regulate the G2/M checkpoint through the inactivation of cyclin-CDK complexes.
In response to any cell damage caused by genotoxic agents, p21 exerts its regulatory effects by nuclear preservation
of cyclin B1-CDK1 and preventing their activation. Notably, several ncRNAs, including lncRNAs and
miRNAs, have been shown to be involved in tumor initiation and progression through the regulation of the p21
signaling axis. In this review, we discuss the miRNA/lncRNA-dependent mechanisms that regulate p21 and
their effects on gastrointestinal tumorigenesis. A better understanding of the regulatory effects of ncRNAs on
the p21 signaling may help to discover novel therapeutic targets in gastrointestinal cancer.
Apatinib Inhibits Bladder Cancer through Suppression of the VEGFR2-
PI3K-AKT Signaling Pathway as Revealed by Network Pharmacology and
in vitro Experimental Verification
Authors: Wang Weiwei , Chen Lin , Yang Jin , Hu Dandan , Yang Yafei , Dong Taotao , Long Xiaoming , Zou Yujian , Li Jia , Ma Xudong , Dai Wenbin , Zhou Xin , Chen Bo , Su Yao 
Published: March 2023

Aims:
This study aimed to evaluate the underlying pharmacological mechanisms of
Apatinib anti-bladder cancer via network pharmacology and experimental verification.

Methods:
Network pharmacology was used to screen the possible signaling pathways of Apatinib
in bladder cancer, and the most likely pathway was selected for in vitro validation. CCK-8 and colony
formation assay were used to detect the effect of Apatinib on the proliferation of bladder cancer
cells. Hoechst staining and flow cytometry detected apoptosis of bladder cancer cells induced
by Apatinib. Western blot was performed to distinguish the effect of Apatinib on the expression
levels of key targets.

Results:
Apatinib can affect many signaling pathways and the correlation of the PI3K-AKT
signaling pathway was the greatest. In vitro experiments showed that Apatinib could inhibit
bladder cancer cell proliferation, induce apoptosis, and up-regulate the expression of apoptosisrelated
proteins Cleaved-PARP and down-regulate the expression of Bcl-2. Furthermore, Apatinib
could decrease the protein expression of VEGFR2, P-VEGFR2, P-PI3K and P-AKT.

Conclusions:
Apatinib could promote apoptosis of bladder cancer cells by inhibiting the VEGFR2-
PI3K-AKT signaling pathway.