bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sfxn5 Regulation of Actin Polymerization for Neutrophil Spreading Depends on a Citrate–Cholesterol–PI(4,5)P2 Pathway
Authors: Zhang Huan , Meng Ling , Liu Yang , Jiang Jinlong , He Zhenting , Qin Jingjing , Wang Cuihong , Yang Meiting , He Ke , Yang Jie , Chen Ketong , He Qinke , Tang Wenwen , Fan Sijia , Ren Chunguang 
Published: June 2023

Abstract
Cell spreading is an initial and critical step in neutrophil adhesion and migration, leading to neutrophil recruitment to inflammatory tissues. Sideroflexin (Sfxn) family proteins are metabolite transporters located in the mitochondrial membrane. Recombinant SFXN5 protein is a citrate transporter in vitro; however, whether Sfxn5 regulates any cellular behavior or function remains unknown. In this study, we found that small interfering RNA transfection or morpholino injection achieving Sfxn5 deficiency in neutrophils significantly decreased neutrophil recruitment in mice and zebrafish, respectively. Sfxn5 deficiency impaired neutrophil spreading and spreading-associated cellular phenotypes, such as cell adhesion, chemotaxis, and ROS production. Actin polymerization is critical for neutrophil spreading, and we found that actin polymerization in spreading neutrophils was partially inhibited by Sfxn5 deficiency. Mechanistically, we observed that the levels of cytosolic citrate and its downstream metabolic products, acetyl-CoA and cholesterol, were decreased in Sfxn5-deficient neutrophils. The levels of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a mediator for the regulation of actin polymerization by cholesterol, were reduced in the plasma membrane of Sfxn5-deficient neutrophils. Exogenous supplementation with citrate or cholesterol partially reversed the reduction in PI(4,5)P2 levels, defective neutrophil actin polymerization, and cell spreading. Altogether, we demonstrated that Sfxn5 maintains cytosolic citrate levels and ensures the synthesis of sufficient cholesterol to promote actin polymerization in a PI(4,5)P2-dependent manner during neutrophil spreading, which is essential for the eventual inflammatory recruitment of neutrophils. Our study revealed the importance of Sfxn5 in neutrophil spreading and migration, thus identifying, to our knowledge, for the first time, the physiological cellular functions of the Sfxn5 gene.
Noneugenol sealers – Thyme oil as an alternative in zinc oxide-based endodontic sealers
Authors: Gnanamuttu Andrew , Veronica Aruna Kumari , Mahalakshmi Krishnan , Sai Shamini , Susila Anand V. 
Published: June 2023

ABSTRACT

Aim:
Thyme has got good anti-inflammatory, antimicrobial, and antifungal properties and is reported to be very effective against oral microflora. The aim of the present study was to compare the antimicrobial activity of zinc oxide and thyme oil to zinc oxide and eugenol.

Methods:
Anti-bacterial efficacy of zinc oxide + eugenol and zinc oxide + thyme oil paste was evaluated against mature Enterococcus faecalis (E. faecalis) dentinal biofilm evaluated by the zone of inhibition (ZOI), biofilm disruption evaluated by colony-forming unit (CFU) reduction, and confocal laser scanning microscopy (CLSM). The red and green fluorescence thresholds were manually set according to their respective intensity and kept consistent for each sample. The ratios of live/dead cells were automatically calculated by the software.

Results:
The results of the present study showed that there was a statistically significant difference in antimicrobial efficacy for thyme oil compared to eugenol as evaluated by ZOI (P = 0.032). Thyme oil was significantly better than eugenol in antimicrobial efficacy according to the percentage of CFU reduction (P = 0.0422) in E. faecalis biofilm. Based on CLSM evaluation, there was no statistically significant difference (P = 0.172) between the two groups.

Conclusion:
Zinc oxide + Thyme oil paste, used in this study has shown promising antibacterial activity against E. faecalis and hence can be used as an alternative sealer.
The Dental Trauma Guide: An evidence-based treatment guide
Authors: Lauridsen Eva , Andersson Lars , Suresh Nandini 
Published: June 2023

ABSTRACT
Dental trauma is complex, as it includes a wide range of different injury types with each type requiring specific considerations. It constitutes the fifth-most prevalent disease, and nearly 900 million individuals from 7 to 65 years of age are affected. The Dental Trauma Guide (DTG) project was initiated by Dr. Jens Andreasen in 2008 with the intention that all dentists around the world should be able to get access to the best available evidence regarding diagnosis, treatment, and prognosis for a patient with dental trauma within a few minutes. The DTG has been a global success, and today, 40,000 colleagues visit the DTG website every year. The website displays the treatment guidelines developed by the International Association of Dental Traumatology as well as visualized with film animations to make it easy and appealing for the users. The dentist can connect to the DTG Copenhagen database to compare his/her case with similar cases from the database, providing guidance to emergency treatment. Furthermore, DTG provides prognosis estimates for each individual injury type, as well as prognosis estimates for teeth with combinations of fracture and luxation injuries. Today, the DTG is a nonprofit organization with the aim of improving the level of care for dental trauma patients worldwide. Therefore, the DTG team has decided to offer free access for students at all dental schools in India.
Comparison between Two da Vinci Surgical Systems in Trifecta and Pentafecta Rates for Robot-Assisted Partial Nephrectomy
Authors: Chen Chih-Heng , Huang Kuo-How , Wang Shuo-Meng , Chow Po-Ming , Chen Pei-Ling , Hong Jian-Hua , Huang Chao-Yuan 
Published: June 2023

Purpose:
Comparative studies on the outcomes of robot-assisted partial nephrectomy (RAPN) procedures conducted using the da Vinci Xi and Si platforms remain sparse. We retrospectively reviewed and compared the Trifecta and Pentafecta rates of the two robotic surgical platforms.

Materials and Methods:
A total of 338 patients who had undergone RAPN using da Vinci Si platform or Xi platform were included. Pentafecta was defined herein as Trifecta (a warm ischemia time <25 min, a negative surgical margin, and the absence of significant perioperative complications) with the addition of estimated glomerular filtration rate preservation of over 90% and no chronic kidney disease stage progression by 12 months postsurgery. Multivariate logistic regression analysis was conducted to identify the predictors of Trifecta and Pentafecta achievement.

Results:
The trifecta and pentafecta rates were higher in the Xi group than in the Si group (65% vs. 29%, P < 0.001 for trifecta; 31% vs. 14%, P = 0.001 for pentafecta). In addition, a significantly shorter average WIT (19 vs. 28 min, P < 0.001) and console time (141 vs. 163 min, P = 0.004) were identified in the Xi group than in the Si group. RAPN conducted using the Xi platform was independently associated with both higher achievement rates of trifecta and pentafecta. In addition, multivariate regression analyses revealed that no history of hypertension, low tumor complexity as measured using the RENAL nephrometry score, anterior orientation of the renal tumor were associated with higher Trifecta rates; while history of diabetes and anterior–posterior renal tumor orientation were the significant predictors of Pentafecta achievement, respectively.

Conclusion:
A significantly higher rates of trifecta and pentafecta achievement were observed in the Xi group than in the Si group. The use of the Xi platform was also significantly associated with low operative times and acceptable complication rates.
High Performance Liquid Chromatography with Size Exclusion Column (HPLC-SEC) Method for Identifying the Major Whey Proteins of Whey Protein Products
Authors: DİAMANTE Lemuel 
Published: June 2023

This study was carried-out: a) to develop a high-performance liquid chromatography with size exclusion column (HPLC-SEC) method for the identification of the major whey proteins from selected whey protein products; and b) use the method to estimate the relative composition of the major whey proteins in different whey protein products.
An HPLC Shimadzu (LC-10AD VP liquid chromatograph) with system controller (SCL-10A VP) equipped with a pump and auto-injector (SIL-10AD VP) and UV-vis detector (SPD-10AV) was used in the identification of whey proteins in standards and whey protein products. The size exclusion column (SEC) was a Yarra 3 µm, SEC-3000 Column, 7.8 mm I.D. x 30 cm with a security guard.
The HPLC-SEC method was successful in identifying the major whey proteins of the different whey protein products.
The β-LG contents had the highest level among the whey proteins for all the whey protein products studied, followed by the α-LA and then IgG in both WPC products. However, the α-LA and IgG of the Procream product had almost the same level which was probably due to a different process used in WPC
All the major whey proteins with the highest pump flowrate had the shortest elution times while the whey proteins with the lowest pump flowrate had the longest elution times. The optimal pump flowrate was 0.75 mL/min since it gave a faster analysis but differentiate the peaks of the different major whey proteins.
PERAN ERGONOMI KOGNITIF UNTUK MENGURANGI HUMAN ERROR DAN BURNOUT PADA PERAWAT UGD SELAMA PANDEMI COVID-19
Authors: Hakim Hakim 
Published: June 2023

Salah satu risiko yang dapat mempengaruhi produktivitas nakes selama pandemi adalah kesehatan mental, termasuk risiko burnout syndrome, ini disebabkan Nakes berada di bawah tekanan tinggi, tetapi tidak ada aturan atau kebijakan untuk melindungi mereka dari perspektif kesehatan mental. Tujuan penelitian ini untuk mengidentifikasi burnout dan human error serta rekomendasi dengan mempertimbangkan aspek ergonomi. Metode identifikasi yang digunakan adalah Human error Assessment and Reduction Technique (HEART), Sampel dalam penelitian ini melibatkan 10 petugas UGD di salah satu Rumah Sakit X Makassar. Hasil penelitian menunjukkan HEP tertinggi terdapat pada aktivitas pemberian tindakan pertolongan pertama yaitu sebesar 0,068. Aktivitas ini merupakan tipe pekerjaan yang membutuhkan skill kerja yang lebih rumit dan kompleks sehingga menyebabkan human unreliability bernilai lebih besar dan berpotensi lebih tinggi untuk terjadi human error . Rekomendasi yang diberikan untuk membantu perawat dalam menghadapi beban kerja yang berat dan beragam sehingga keandalan perawat dalam bekerja dapat meningkat dan burnout dapat dikurangi antara lain dengan menekan faktor lingkungan sekecil mungkin untuk mengurangi beban kerja perawat, menghimbau perawat untuk mulai memonitor situasi dan mengambil langkah antisipasi agar burnout yang dialami tidak bertambah parah serta dengan mengadakan sharing rutin antar perawat, upgrading ilmu pengetahuan dan pengecekan alat kesehatan sebelum digunakan untuk menghindari human error.

One of the risks that can affect the productivity of health workers during the pandemic is mental health, including the risk of burnout syndrome, this is because health workers are under high pressure, but there are no rules or policies to protect them from a mental health perspective. The purpose of this study is to identify burnout and human error as well as recommendations by considering ergonomic aspects. The identification method used is the Human error Assessment And Reduction Technique (HEART). The sample in this study involved 10 emergency room staff at one of the Makassar Hospitals. The results showed that the highest HEP was found in the activity of providing first aid measures, which was 0.068. This activity is a type of work that requires work skills that are more complicated and complex, causing human unreliability to be of greater value and having a higher potential for human error to occur. Recommendations are given to assist nurses in dealing with heavy and varied workloads so that the reliability of nurses at work can be increased and burnout can be reduced, among others, by suppressing environmental factors as small as possible to reduce the workload of nurses, urging nurses to start monitoring the situation and taking anticipatory steps so that the burnout experienced did not get worse and by holding regular sharing between nurses, upgrading knowledge and checking medical devices before use to avoid human error.
Fuzzy Logic and Neural Network-based Risk Assessment Model for Import and Export Enterprises: A Review
Authors: Luo Na , Yu Hua , You Zeqing , Li Yao , Zhou Tunan , Jiao Yuwei , Han Nan , Liu Chenxu , Jiang Zihan , Qiao Shaojie 
Published: June 2023

With the rapid growth in foreign trade business and the continuous expansion of customs functions, the amount of data obtained by customs monitoring systems has drastically increased, and risk management techniques have been widely used in the customs field. Risk assessment, as an important part of risk management, can help departments of customs to understand the possibility and impact of risks in advance. Assessing the risks of import and export enterprises is an important task for customs. However, the assessment is challenging because of the large-scale of data, rapidly changing information, and inherent fuzziness. To address this issue, this survey summarizes the existing risk assessment methods, combines the preliminaries of fuzzy logic and neural networks, and applies the representative risk assessment models in the risk assessment of import and export enterprises. The risk assessment models based on fuzzy logic and neural networks can handle vague and uncertain data and improve the efficiency of risk assessment through adaptive and learning capabilities. The combination of theoretical and simple case suggests that the risk assessment method based on fuzzy logic and neural networks has good feasibility for import and export enterprises and provides valuable references for developing effective risk assessment models of import and export enterprises in the future.

Received: 30 April 2023 | Revised: 14 June 2023 | Accepted: 16 June 2023

Conflicts of Interest:
Shaojie Qiao is the Editor-in-Chief for Journal of Data Science and Intelligent Systems and was not involved in the editorial review or the decision to publish this article. The authors declare that they have no conflicts of interest to this work.
Identification of high-risk groups of falciparum malaria in western region of Ghana: The predictive value of ABO Blood group typology
Authors: Ampomah Paulina , Buadii Erica , Aboagye Benjamin 
Published: June 2023

Background: Several studies have linked malaria to ABO blood groups with still others reporting insignificant association between ABO blood group system and malaria. Blood group ‘O’ has been shown to confer protection against severe malaria by studies in various populations but indecisive reports have been given about non-O blood groups in relation to their protection or vulnerability to severe malaria.
Objective: The present study sought to investigate the ABO blood group typology and the risk of developing severe malaria.
Materials and Methods: A total of 280 participants (140) with P. Falciparum malaria patients and 140 healthy controls) screened for ABO blood groups by Tile method were enrolled into the study. Thick and thin blood films stained with 10 % Giemsa were prepared for falciparum – infected individuals and their full blood counts obtained from the haematology analyzer (Cell Dyn 1800, Abbot Diagnostic Division, USA). Parasite counts were categorized into severe and uncomplicated malaria and further grouped into varying degrees of parasitaemia. The effects of parasite densities on some haematological parameters were then studied. Severe malaria was defined as hyperparasitaemia, malarial anaemia and thrombocytopenia.
Results: The frequency of blood group ‘A’ was significantly higher in patients with severe malaria compared to other blood groups (p = 0.042). Blood groups ‘A’ and ‘B’ showed higher parasite densities while ‘A’ and ‘AB’ blood groups revealed low platelet counts. Anaemia was severe in blood groups ‘A’ and ‘O’ (p ≤ 0.05). Previous studies including this current one highlighted the protected nature of blood group ‘O’ to severe malaria (p ≤ 0.05).
Conclusion: The present study provides the evidences that, individuals of blood group ‘A’ are highly susceptible to malaria infection and inferably with an increased risk of developing severe malaria than the other blood groups.
Transfusion-related adverse reactions: An update from a district hospital in Ghana
Authors: Boachie Joseph , Yahaya Piiga Jemila , Esi Donkoh Irene , Boakye Amponsah Seth , Akuoko Essuman Mainprice , Dadzie Esther , Boye Alex 
Published: June 2023

Background: Transfusion therapy is indispensable for effective emergency medicine practice. However, in resource-poor settings transfusion therapy is suspected to be practiced in a manner that may be contributory to morbidity and mortality among blood recipients. Yet, there exists a wide information gap, with respect to incidence of transfusion-related adverse reactions secondary to inappropriate blood prescription.
Objective: This study assessed incidence of transfusion-related adverse reactions in a district hospital in Ghana.
Materials and Methods: A hospital-based cross-sectional study was conducted from December 2020 to January 2021. Patients attending a district hospital in Kumasi, Ghana who were prescribed blood transfusion were recruited into the study after oral/written consent. Patients’ demographic data, blood components and units of blood requested as well as the ward of patients were recorded. Records of patient’s full blood count (FBC), body temperature, blood pressure and transfusion-related adverse reactions were extracted from their medical folders.
Results: Out of 200 patients, 169 were transfused with whole blood while 31 patients received packed red cells. Majority (48%) of the patients were transfused with ≤1 unit whereas 10% received 3 units of blood. Anaemia (Hb = 6.13 g/dl) and leukocytosis (WBC = 13.83 x 103/µL) were observed in the haematological parameters in which the MCV and lymphocyte count differed significantly in males compared to females. The overall incidence of transfusion-related adverse reactions was 47.5%. Of the 169 patients who received whole blood, more than half (53.8%) experienced more than one of the transfusion-related adverse reactions that were monitored. Finally, patients within the ages of 19-29 years had the highest incidence of transfusion reaction (30.5%) and overall, the number of units administered was significantly associated with the incidence of transfusion reaction (p<0.011). Conclusion: Transfusion-related adverse reaction was common at the study setting. This could be attributed to inappropriate administration of blood and blood products. Strict adherence to World Health Organization recommendations on transfusion therapy needs to be encouraged and supervised in the study setting>< 0.011)
Conclusion: Transfusion-related adverse reaction was common at the study setting. This could be attributed to inappropriate administration of blood and blood products. Strict adherence to World Health Organization recommendations on transfusion therapy needs to be encouraged and supervised in the study setting.
FUNGSI DAN CABARAN BARUK SEBAGAI WARISAN BUDAYA BIDAYUH
Authors: Atar Suzanne Anak , Ansely Angelina , Bala Bilcher 
Published: June 2023

Baruk merupakan sebuah rumah khas yang berfungsi sebagai balai raya bagi etnik Bidayuh di Sarawak. Pada zaman dahulu, baruk mempunyai pelbagai fungsi sosial dan politik. Setiap perkampungan masyarakat Bidayuh mempunyai baruk masing-masing. Pada masa ini, jumlah baruk semakin berkurangan, sekali gus telah mengalami perubahan dari segi fungsi. Baruk yang masih kekal atau yang dibina semula bagi menjadi mercu tanda sesebuah kampung, monumen lambang warisan budaya dan identiti bagi masyarakat Bidayuh. Artikel ini akan membincangkan fungsi dan cabaran yang dihadapi oleh baruk pada hari ini. Kajian artikel ini menggunakan sumber primer, sekunder dan lisan serta kajian kerja lapangan. Makalah ini bertujuan untuk menjelaskan permasalahan tentang perubahan yang berlaku ke atas baruk sebagai sebuah institusi budaya bagi kaum Bidayuh.Baruk is a special house that serves as a community hall for the Bidayuh ethnic groups in Sarawak. In traditional times,Â baruk have a variety of social and political functions. Each village of Bidayuh has its own baruk. Nowadays, the number of baruk decreases and there has been a change in function. Thebarukwhich still exists or has been rebuilt, become as the landmark of a village, and is a monument symbol of cultural heritage and identityÂ for the Bidayuh community. This article will discuss the current functions and challenges faced by the baruk. The study of this article uses primary, secondary, and oral sources as well as field work studies. This article aims to clarify the issues of change that apply to the baruk as a cultural institution for the Bidayuh community.
Hubungan Personal Hygiene (Kebersihan Handuk) dengan Kejadian Scabies di Pondok Pesantren Nizhomul Hikmah Desa Tamiang
Authors: Lubis Juliana 
Published: June 2023

Tempat-tempat yang menjadi favorit bagi sarcoptes scabei adalah daerah-daerah lipatan kulit seperti telapak tangan, kaki, selangkangan, lipatan paha, lipatan perut, ketiak dan daerah vital. Penyakit scabies disebabkan faktor kebersihan yang kurang dipelihara secara baik. Alat tidur berupa kasur, sprei, bantal, tempat tidur dan kondisi kamar yang pengap, dapat memicu terjadinya gatal-gatal. Walaupun tidak sampai membahayakan jiwa, penyakit scabies perlu mendapatkan perhatian karena tingkat penularannya yang tinggi serta dapat mengganggu kenyamanan, terutama saat tidur dimalam hari dan gangguan konsep diri. Penelitian ini bertujuan untuk mengetahui hubungan personal hygiene (Kebersihan Handuk) dengan kejadian scabies di Pondok Pesantren Nizhomul Hikmah Desa Tamiang. Penelitian ini adalah penelitian yang bersifat analitik dengan desain cros sectional, dengan data primer, dengan menggunakan lembar observasi. Populasi 43 orang santri/wati kelas 2 tsanawiyah dan pengambilan sampel dengan total sampling yaitu 43 orang santri/wati kelas 2 tsanawiyah dan di analisa dengan uji chi-square.Hasil penelitian menunjukkan ada hubungan personal hygiene (kebersihan handuk) dengan kejadian skabies di pondok pesantren Nizhomul Hikmah Desa Tamiang dengan nilai p=0.002<p=0.05. Dapat meningkatkan pelayanan kesehatan dan memberikan informasi kepada siswa tentang kebersihan diri dan lingkungan pondok pesantren agar terhidar dari penyakit scabies.
Kata Kunci : Personal hygiene, scabies
ABSTRACT
The places that are a favorite for Sarcoptes scabei are areas of skin folds such as the palms of the hands, feet, groin, groin, abdominal folds, armpits and vital areas. Scabies disease is caused by hygiene factors that are not properly maintained. Sleeping equipment in the form of mattresses, sheets, pillows, beds and stuffy room conditions can trigger itching. Although it is not life-threatening, scabies disease needs attention because of its high transmission rate and can interfere with comfort, especially during sleep at night and disturbances in self-concept. This study aims to determine the relationship of personal hygiene (towel cleanliness) with the incidence of scabies at the Nizhomul Hikmah Islamic Boarding School, Tamiang Village. This research is an analytic study with a cross sectional design, with primary data, using an observation sheet. The population is 43 students in grade 2 tsanawiyah and sampling with a total sampling of 43 students in grade 2 tsanawiyah and analyzed by chi-square test. The results showed that there was a relationship between personal hygiene (towel cleanliness) and the incidence of scabies in the Nizhomul Hikmah Islamic boarding school, Tamiang Village, with a value of p=0.002<p=0.05. Can improve health services and provide information to students about personal hygiene and the environment of Islamic boarding schools to avoid scabies disease.Keywords: Personal hygiene, scabies