bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fast dynamic softening of hydrogels increases endogenous cell forces by accumulating mechanical signalling molecules
Authors: Yang Jiapeng , Wang Peng , Zhang Yu , Zhang Man , Sun Qian , Chen Huiyan , Dong Liang , Chu Zhiqin , Xue Bin , Hoff Wouter , Zhao Changsheng , Wang Wei , Wei Qiang , Cao Yi 
Published: May 2023

Abstract
The rigidity of extracellular matrices (ECMs) is an important factor defining cellular mechanotransduction. Under static conditions, cells typically generate higher endogenous forces on stiff substrates than on soft substrates. However, as ECMs are characteristic of a nonlinear mechanical response, their rigidities constantly change upon tissue deformation. It remains largely unknown the mechanisms by which cells respond to dynamic rigidity changes. In this study, we develop a photoresponsive protein hydrogel in which its mechanical properties are rapidly and reversibly switched between stiff and soft states. Counterintuitively, we discover that the cyclic softening of the substrate at appropriate frequencies increases the endogenous forces and the mechanical transduction. These results are reconciled by a quantitative model considering the kinetic coupling of both the dynamics of the molecular clutch and the engagement of the mechanical signalling proteins. Our findings suggest that dynamic rigidity changes are important design parameters for synthetic biomaterials engineered for cell culture and tissue engineering applications.
Selective Exposure Reduces Voluntary Contributions: Experimental Evidence from the German Internet Panel
Authors: Innocenti Federico , Rohde Linnéa Marie 
Published: May 2023

Abstract
Can strategic information acquisition harm the provision of a public good? We investigate this question in an incentivized online experiment with a large sample of the German population. The marginal returns of the public good are uncertain: it is either socially efficient to contribute or not. In the information treatment, participants can choose between two information sources with opposite biases. One source is more likely to report low marginal returns, whereas the other is more likely to report high marginal returns. We find that information avoidance is a minor phenomenon. Most participants select the source biased towards reporting low marginal returns, independent of their prior beliefs. As a result, the information treatment reduces contributions and increases free-riding. When contributing is socially efficient, the information treatment reduces social welfare. We find that social preferences guide information acquisition: socially-oriented participants are more likely to acquire information and select the source that is biased towards reporting low marginal returns.
JEL Classification: C99, D12, D61, D83, H41
Avoiding overestimations of mitigation potential and costs of CCUS in China’s steel industry
Authors: Wen Zongguo , Wang Yihan , Chen Lei , Xu Mao , Dinga Christian Doh , Li Jia 
Published: May 2023

Abstract
Carbon capture, utilization, and storage (CCUS) is promising for carbon neutrality vision in steel industry. Previous work has overestimated both mitigation potentials and economic costs, hindering the long-term planning of CCUS. Here, we propose a soft-linking approach, combining the industry decarbonization pathway and emission source-sink matching models, to avoid both overestimations in China’s steel industry. Results show the emissions of China’s steel industry are estimated to decrease by 38.9-55.2% under three scenarios before retrofitted by CCUS. The optimized CCUS layouts have mitigation effects of 438.01-529.02 Mt, meeting the requirements of the industrial –level decarbonization pathways. The specific costs of CCUS are 138-150 CNY/t, 29-65% lower than the unselected sources. CCUS will increase the energy and water consumption by 25%+ with uneven distribution, exacerbating local resource scarcity problems. The methodology and findings can clarify the mitigation effects and costs of the CCUS technology to set proper long-term planning for its promotion.
Acute kidney injury induced by angiotensin-converting enzyme inhibitor in a kidney transplant recipient: a case report
Authors: Meng Yang , Hongwen Zhao , Dongkai Xiao , Xiaosong Xu 
Published: May 2023

Abstract
Background: We described our medical records in details combined with some published literature, to discuss the suitable population and timing of Angiotensin-Converting Enzyme Inhibitor (ACEI) in kidney transplant recipients(KTRs), and summarize the diagnosis and treatment of acute kidney injury in KTRs caused by ACEI provides a reference for the more rational application of ACEI in kidney transplant recipients in the future.
Case presentation: This study describes a rare case of acute kidney injury in a kidney transplant recipient with an ACEI. A 37-year-old man underwent a kidney allograft from a brain-dead donor 7 months ago. The operation was succeed, and the postoperative serum creatinine fluctuation was 200-300umol/L. One week before admission, the patient adjusted the antihypertensive drugs under the guidance of the doctor, and changed the nifedipine to the benazepril due to his poor blood pressure control. The next day after the change, he developed gastrointestinal symptoms dominated by nausea. On the sixth day after the change, his gastrointestinal symptoms were aggravated, the nausea, vomiting, and decreased urine output happened. The patient rushed to our hospital for seeking help. There were no obvious abnormality found when physical examination. Laboratory examination showed that creatinine grow to 859.13 umol/L. The transplanted kidney under ultrasound didn’t showe any abnormality. After screening his medical history, we considered that the acute kidney injury induced by ACEI benazepril might happen. After we stop the administration of benazepril, the serum creatinine gradually decreased to 241.20umol/L. This is the first report of acute kidney injury caused by ACEI in a KTR.
Conclusion: In KTRs, non-early use of ACEI/ARB requires regular monitoring of serum creatinine levels. These drugs should not be administered once creatinine levels have risen.
A Higher Radix Architecture for Quantum Carry-lookahead Adder
Authors: Wang Siyi , Baksi Anubhab , Chattopadhyay Anupam 
Published: May 2023

Abstract
In this paper, we propose an efficient quantum carry-lookahead adder based on the higher radix structure. For the addition of two n-bit numbers, our adder uses O(n)-O(n/r) qubits and O(n)+O(n/r) T gates to get the correct answer in T-depth O(r)+O(log(n/r)), where r is the radix.

Quantum carry-lookahead adder has already attracted some attention because of its low T-depth. Our work further reduces the overall cost by introducing a higher radix layer. By analyzing the performance in T-depth, T-count, and qubit count, it is shown that the proposed adder is superior to existing quantum carry-lookahead adders. Even compared to the Draper out-of-place adder which is very compact and efficient, our adder is still better in terms of T-count.
The effect of whole blood logistics on neutrophil non-specific activation and kinetics ex vivo
Authors: Li Chao , Farooqui Mehtab , Yada Ravi Chandra , Cai Joseph B. , Huttenlocher Anna , Beebe David J. 
Published: May 2023

Abstract
While the exquisite sensitivity of neutrophils enables their rapid response to infection in vivo; this same sensitivity complicates the ex vivo study of neutrophils. Handling of neutrophils ex vivo is fraught with unwanted heterogeneity and alterations that can diminish the reproducibility of assays and limit what biological conclusions can be drawn. There is a need to better understand the influence of ex vivo procedures on neutrophil behavior to guide improved protocols for ex vivo neutrophil assessment to improve inter/intra-experimental variability. Here, we investigate how whole blood logistics (i.e., the procedure taken from whole blood collection to delivery of the samples to analytical labs and storage before neutrophil interrogation) affects neutrophil non-specific activation (i.e., baseline apoptosis and NETosis) and kinetics (i.e., activation over time). All the experiments (60+ whole blood neutrophil isolations across 36 blood donors) are performed by a single operator with optimized isolation and culture conditions, and automated image analysis, which together increase rigor and consistency. Our results reveal: i) Short-term storage (<8 h) of whole blood does not significantly affect neutrophil kinetics in subsequent two-dimensional (2D) cell culture; ii) Neutrophils from long-term storage (>24 h) in whole blood show significantly higher stability (i.e., less non-specific activation) compared to the control group with the isolated cells in 2D culture. iii) Neutrophils have greater non-specific activation and accelerated kinetic profiles when stored in whole blood beyond 48 h.
Study on the Law of Surface Subsidence in Layered Mining of Thick Coal Seam with Medium Hard Roof
Authors: Liu Xiugang , Wei Fei , Tan Zhixiang , Jiang Zaibing , Wang Yi , Zhang Jie 
Published: May 2023

Abstract

The thick coal seam carried out by layered mining, the influence range of the overlying rock layer is relatively larger than that of the single-layer mining, and the surface deformation is more intense. At present, the research of thick coal seam mining is mainly concentrated within 15m, and there are relatively few studies on coal seams with a cumulative mining thickness greater than 15m. In order to study the surface sinking law of coal seam mining with a cumulative mining thickness greater than 15m, this paper takes the Mengba as the research object to simulate the change of the surface sinking coefficient after layered mining under the condition that the thickness of the medium hard roof plate and single-layer mining is 5m. In order to verify the correctness of the model, the simulation results are combined with the measured data after the first and second layers of the 1210 working surface of the surface observation station, and the maximum relative error between the simulation results and the measured results was 2.7%, which was less than the specified limit difference of 5%.It shows that the surface sinking coefficient q of stratified mining has the characteristics of segmentation. When the cumulative mining thickness is less than 25m, q is positively correlated with the mining thickness. When the cumulative mining thickness is greater than 25m, the q value will tend to be a fixed value and will no longer change with the increase of mining thickness. Finally, the calculation formula between the ground subsidence coefficient and cumulative mining thickness of layered mining under the condition of medium-hard roof is fitted,which provides a parameter basis for the mining of coal seams in similar mines.
Solar-to-Chemical Conversion in the Near-Infrared Region for H2O2 Production via a One-Step Two-Electron Transfer Pathway
Authors: Yang Guidong , Ou Honghui , Zhao Chao , Li He , Li Yang , Yan Xiaoqing , Lin Bo 
Published: May 2023

Abstract
Solar-to-chemical conversion by semiconductor photocatalysis is a sustainable strategy to produce renewable energy. Here, we demonstrate that the generation of hydrogen peroxide (H2O2) via a typical one-step two-electron reduction of O2 by light-induced electrons could provide a new pathway for near-IR light catalysis. The proof-of-concept material is an oxygen-enriched borocarbonitride ceramic photocatalyst (BCNO). Density functional theory analysis suggests that the intermediates of O2 reduction of BCNO can be reasonably adjusted by the incorporation of O atoms. Consistent with the theoretical results, BCNO can generate H2O2 through a typical one-step two-electron reduction of O2 under near-infrared light. Together with the loading of a Co single atom cocatalyst for enhanced water oxidation rate, the apparent quantum efficiency values at 850 nm of the optimal BCNO for H2O2 evolution were estimated to be ~0.8 %, and the solar-to-chemical conversion (SCC) efficiency can reach 0.6 %. Our research reveals an important reaction pathway for the generation of H2O2, emphasizing that precise control of the energy band structure and surface structure of the photocatalyst is essential for achieving efficient conversion of solar-to-chemical.
Evaluation of children between 6-12 years of age with vitamin B12 deficiency in terms of slow cognitive tempo
Authors: Kilicaslan Cengizhan , Saglam Ebru 
Published: May 2023

Abstract
Background Slow cognitive tempo (SCT) is a disorder characterized by two symptomatic dimensions: motor symptoms such as sluggishness and slow movement, and such cognitive problems as daydreaming, appearing sleepy, slowed behavior/thinking, and confusion. Albeit its unclear etiology, SCT is considered multifactorial, as in attention deficit hyperactivity disorder (ADHD). Here, we aimed to investigate the relationship between vitamin B12 deficiency, one of the conditions causing neurodevelopmental retardation, and SCT.Methods Diagnosed with vitamin B12 deficiency, 52 patients without additional vitamin deficiency and psychiatric diseases, and 52 controls without vitamin deficiency and any diseases were included in the study. While the Conners' Parent Rating Scale-Revised Long (CPRS-R:L) and the Barkley Child Attention Scale (BCAS) were performed with cases’ parents, their teachers underwent the Conners' Teacher Rating Scale-Revised Long (CTRS-R:L) and BCAS. The scores were also evaluated statistically.Results Among B12 deficiency patients, parental education was found lower, and the number of siblings was higher than controls. The risk of patients’ daydreaming scores with vitamin B12 deficiency was 1.4 times higher than that of the controls. Given the investigation of SCT concerning sluggishness and daydreaming scores, a significant difference was detected in terms of sex difference (p = 0.008), and girls were seen to have a higher total score.Conclusion In families with a higher number of siblings and a lower level of parental education, vitamin B12 deficiency is more commonly witnessed, and the symptoms of SCT should be considered to develop in those with B12 deficiency, without severe neurological and hematological findings.
Natural product Shi-Bi-Man regulates lactic acid metabolism and drives hair follicle stem cell activation to promote hair regeneration
Authors: Du Haojie , Zhang Tao , Wang Qiao , Cao Xinran , Zheng Huiwen , Li Jiabin , Qu Jiao , Guo Lehang , Sun Yang 
Published: May 2023

Abstract
Background : As a supplement for promoting hair health, Shi-Bi-Man (SBM) is a natural product comprising various traditional Chinese medicines. Though SBM has been reported to promote hair regeneration, its molecular mechanisms remains unclear. Cynomolgus monkeys (Macaca fascicularis) are non-human primates with a gene expression profile similar to that of humans. The purpose of this research is to evaluate the effect of SBM on promoting hair regeneration in cynomolgus monkeys and to reveal the underlying mechanism.
Methods : The effect of SBM on hair regeneration was observed by skin administration on 6 cynomolgus monkeys with artificial back shaving. The molecular mechanism of SBM was studied using single-cell RNA sequencing (scRNA-seq) in combination with quantitative polymerase chain reaction (qPCR) detection for gene transcription level, and immunofluorescence staining verification for protein level.
Results : SBM significantly promoted hair regeneration on cynomolgus monkeys, increased hair follicle number and facilitated hair follicle development. Single-cell RNA revealed an increase in the number of hair follicle stem cells (HFSCs) with a higher activation state, as evidenced by the higher expression of activation marker LDHA related to metabolism and the proliferation marker MKI67. Immunofluorescence analysis at the protein level and qPCR at the mRNA level have confirmed the sequencing data. Cellchat analysis revealed an enrichment of ligand-receptor pairs involved in intercellular communication in Laminin-related pathways.
Conclusion : SBM significantly promoted hair regeneration on cynomolgus monkeys. Mechanically, SBM could promote LDHA expression and drive HFSC activation. The cell level analysis suggested that SBM promoted the proliferation and differentiation of HFSCs by affecting interaction between HFSCs and others on Laminin-related pathways. Keywords : Hair follicle stem cell activation, Shi-Bi-Man, cynomolgus monkey, single-cell RNA sequencing, LDHA, Laminin pathway.
Evaporation-induced hydrodynamics control plasmid transfer during surface-associated microbial growth
Authors: Johnson David , Ruan Chujin , Borer Benedict , Ramoneda Josep , Wang Gang 
Published: May 2023

Abstract
Droplet evaporation is a general process in unsaturated environments that results in micro-scale hydrodynamic flows that determine the spatial distributions of microbial cells across surfaces. These spatial distributions can have significant effects on the development and functioning of surface-associated microbial communities, with consequences on important processes such as the spread of plasmids. Here, we experimentally quantified how evaporation-induced hydrodynamic processes modulate the initial deposition patterns of microbial cells (via the coffee ring effect and Marangoni convection) and how these patterns control the spread of an antibiotic resistance-encoding plasmid during surface-associated growth. We found that plasmid spread is a function of the initial density of cells deposited along the droplet periphery, which is a manifestation of the coffee ring effect. Using an individual-based computational model, we systematically linked how the different initial cell deposition patterns caused by different relative strengths of the coffee ring effect and Marangoni convection determine the extent of plasmid transfer during surface-associated growth. Our study demonstrates that evaporation-induced hydrodynamic processes that are common in nature can alter crucial ecological properties of surface-associated microbial communities and control the proliferation of plasmids, with consequences on the spread of antibiotic resistance and other plasmid-encoded traits.
Control of Polymers’ Amorphous-crystalline Transition for Hydrogel Bioelectronics Miniaturization and Multifunctional Integration
Authors: Rao Siyuan , Huang Sizhe , Liu Xinyue , Lin Shaoting , Glynn Christopher , Felix Kayla , Sahasrabudhe Atharva , Maley Collin , Xu Jingyi , Chen Weixuan , Hong Eunji , Crosby Alfred , Wang Qianbin 
Published: May 2023

Abstract

Bioelectronic devices made of soft elastic materials exhibit motion-adaptive properties suitable for brain-machine interfaces and for investigating complex neural circuits. While two-dimensional microfabrication strategies enable miniaturizing devices to access delicate nerve structures, creating 3D architecture for expansive implementation requires more accessible and scalable manufacturing approaches. Here we present a fabrication strategy through the control of metamorphic polymers’ amorphous-crystalline transition (COMPACT), for hydrogel bioelectronics with miniaturized fiber shape and multifunctional interrogation of neural circuits. By introducing multiple cross-linkers, acidification treatment, and oriented polymeric crystalline growth under deformation, we observed about an 80% diameter decrease in chemically cross-linked polyvinyl alcohol (PVA) hydrogel fibers, stably maintained in a fully hydrated state. We revealed that the addition of cross-linkers and acidification facilitated the oriented polymetric crystalline growth under mechanical stretching, which contributed to the desired hydrogel fiber diameter decrease. Our approach enabled the control of hydrogels’ properties, including refractive index (RI 1.37-1.40 at 480 nm), light transmission (> 96%), stretchability (95% - 111%), and elastic modulus (10-63 MPa). To exploit these properties, we fabricated step-index hydrogel optical probes with contrasting RIs and applied them in optogenetics and photometric recordings in the mouse brain region of the ventral tegmental area (VTA) with concurrent social behavioral assessment. To extend COMPACT hydrogel multifunctional scaffolds to assimilate conductive nanomaterials and integrate multiple components of optical waveguide and electrodes, we developed carbon nanotubes (CNTs)-PVA hydrogel microelectrodes for hindlimb muscle electromyographic and brain electrophysiological recordings of light-triggered neural activities in transgenic mice expressing Channelrhodopsin-2 (ChR2).
Micro-CT analysis of the incidence and treatment of mandibular first premolars with C-shaped canal system in a Chinese population
Authors: Zhang Yimeng , Weng Xunben , Fu Yu , Qi Xuekai , Pan Yihuai , Zhao Yu 
Published: May 2023

Abstract
Objectives
The purpose of this study was to determine the prevalence of C-shaped root canal system in mandibular first premolar in Chinese population and to analyze the anatomical characteristics by Cone-beam computed tomography (CBCT) and Micro–computed tomography (Micro-CT) together.
Methods and materials
CBCT images of 760 patients (1520 mandibular first premolars) were evaluated for the prevalence of C-shaped root canal system and the correlation with gender, age and position. 66 mandibular first premolars with C-shaped root canal system were scanned by Micro-CT and analyzed for morphologic features of the root canal anatomy (radicular groove, C-shaped root canal categories, accessory and connecting canals, apical foramina and accessory foramina).
Results
C-shaped root canal system was found in 16.9% of mandibular first premolars. The minimum mesial wall thickness was mostly measured at the lingual site (69.7%). Among the cross-sectional C-shaped root canal system classifications, the highest prevalence was C2 type (41.5%). In the 3D C-shaped root canal system classification, the highest prevalence was S type (34.8%). Accessory canals were observed in 48.5% of the samples, mostly in the middle region. The presence of 1-3 variable connecting canals was observed in 42.4% of the samples. The number of apical foramina was predominantly 1 (40.9%).
Conclusions
The incidence of C-shaped root canal system in mandibular first premolars was 16.9% in the Chinese population. It mostly had anatomical features such as deep root surface grooves, with C2 type and S type being the most prevalent.
Ultracompact mirror device for forming 20-nm achromatic soft-X-ray focus toward multimodal and multicolor nanoanalyses
Authors: Shimamura Takenori , Takeo Yoko , Moriya Fumika , Kimura Takashi , Shimura Mari , Senba Yasunori , Kishimoto Hikaru , Ohashi Haruhiko , Shimba Kenta , Jimbo Yasuhiko , Mimura Hidekazu 
Published: May 2023

Abstract
Nanoscale soft-X-ray microscopy is a powerful analysis tool in biological, chemical, and physical sciences. To enhance its probe sensitivity and leverage multimodal soft-X-ray microscopy, precise achromatic focusing devices, which are challenging to fabricate, are essential. Here, we develop an ultracompact Kirkpatrick-Baez (ucKB) mirror, which is ideally capable of high-performance nanofocusing for broadband-energy X-rays. We apply our advanced fabrication techniques and short-focal-length strategy to realize diffraction-limited focusing over the entire soft-X-ray range. We achieve a focus size of 20.4 nm at 2 keV, representing a significant improvement in achromatic soft-X-ray focusing. The ucKB mirror extends soft-X-ray fluorescence microscopy by producing a bicolor nanoprobe with a 1- or 2-keV photon energy. We propose a subcellular chemical mapping method allowing a comprehensive analysis of specimen morphology and the distribution of light elements and metal elements. ucKB mirrors will improve soft-X-ray nanoanalyses by facilitating photon-hungry, multimodal, and polychromatic methods, even with table-top X-ray sources.