bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining the relationship between heatwaves and fatal drowning: a case study from Queensland, Australia
Authors: Peden Amy E , Mason Hannah M , King Jemma Chandal , Franklin Richard Charles 
Published: September 2023

BackgroundGlobally, drowning is a leading cause of injury-related harm, which is heavily impacted by environmental conditions. In Australia, fatal unintentional drowning peaks in summer, yet the impact of prolonged periods of hot weather (heatwave) on fatal drowning has not previously been explored.MethodsUsing a case-crossover approach, we examined the difference in drowning risk between heatwave and non-heatwave days for the Australian state of Queensland from 2010 to 2019. Heatwave data, measured by the excess heat factor, were acquired from the Bureau of Meteorology. Incidence rate ratios (IRRs) were calculated by sex, age of drowning decedent, category of drowning incident (International Classification of Diseases−10 codes) and heatwave severity. Excess drowning mortality during heatwaves was also calculated.ResultsAnalyses reveal increased fatal drowning risk during heatwave for males (IRR 1.22, 95% CI 0.92 to 1.61), people aged 65+ years (IRR 1.36, 95% CI 0.83 to 2.24), unintentional drowning (IRR 1.28, 95% CI 0.98 to 1.69) and during severe heatwaves (IRR 1.26, 95% CI0.88 to 1.82). There were 13 excess drowning deaths due to heatwave over the study period.DiscussionThe findings confirm an increased risk of fatal drowning during heatwaves. With increased likelihood and severity of heatwaves, this information should be used to inform drowning prevention, in particular the timing of public awareness campaigns and patrolling of supervised aquatic locations.ConclusionsWater safety and patrolling organisations, as well as first responders, need to prepare for more drowning deaths during heatwave conditions. In addition, drowning prevention education ahead of heatwaves is needed for recreational swimmers, and older people, particularly those with comorbidities which may be further exacerbated by a heatwave.
Performance evaluation of a CRISPR Cas9-based selective exponential amplification assay for the detection of KRAS mutations in plasma of patients with advanced pancreatic cancer
Authors: Shen Yue , Zhang Xiaoling , Zhang Liyi , Zhang Zuoying , Lyu Bao , Lai Qian , Li Qinglin , Zhang Yuhua , Ying Jieer , Song Jinzhao 
Published: September 2023

AimsPancreatic ductal adenocarcinoma (PDAC) is highly malignant, with shockingly mortality rates. KRAS oncoprotein is the main molecular target for PDAC. Liquid biopsies, such as the detection of circulating tumour DNA (ctDNA), offer a promising approach for less invasive diagnosis. In this study, we aim to evaluate the precision and utility of programmable enzyme-based selective exponential amplification (PASEA) assay for rare mutant alleles identification.MethodsPASEA uses CRISPR-Cas9 to continuously shear wild-type alleles during recombinase polymerase amplification, while mutant alleles are exponentially amplified, ultimately reaching a level detectable by Sanger sequencing. We applied PASEA to detect KRAS mutations in plasma ctDNA. A total of 153 patients with stage IV PDAC were enrolled. We investigated the relationship between ctDNA detection rates with various clinical factors.ResultsOur results showed 91.43% vs 44.83% detection rate in patients of prechemotherapy and undergoing chemotherapy. KRAS ctDNA was more prevalent in patients with liver metastases and patients did not undergo surgical resection. Patients with liver metastases prior to chemotherapy showed a sensitivity of 95.24% (20/21) with PASEA. Through longitudinal monitoring, we found ctDNA may be a more accurate biomarker for monitoring chemotherapy efficacy in PDAC than CA19-9.ConclusionsOur study sheds light on the potential of ctDNA as a valuable complementary biomarker for precision targeted therapy, emphasising the importance of considering chemotherapy status, metastatic sites and surgical history when evaluating its diagnostic potential in PDAC. PASEA technology provides a reliable, cost-effective and minimally invasive method for detecting ctDNA of PDAC.
Eicosanoid-Regulated Myeloid ENaC and Isolevuglandin Formation in Human Salt-Sensitive Hypertension
Authors: Ertuglu Lale A. , Pitzer Mutchler Ashley , Jamison Sydney , Laffer Cheryl L. , Elijovich Fernando , Saleem Mohammad , Blackwell Daniel J. , Kryshtal Dmytro O. , Egly Christian L. , Sahinoz Melis , Sheng Quanhu , Wanjalla Celestine N. , Pakala Suman , Yu Justin , Gutierrez Orlando M. , Kleyman Thomas R. , Knollmann Björn C. , Ikizler T. Alp , Kirabo Annet 
Published: September 2023

BACKGROUND:
The mechanisms by which salt increases blood pressure in people with salt sensitivity remain unclear. Our previous studies found that high sodium enters antigen-presenting cells (APCs) via the epithelial sodium channel and leads to the production of isolevuglandins and hypertension. In the current mechanistic clinical study, we hypothesized that epithelial sodium channel–dependent isolevuglandin-adduct formation in APCs is regulated by epoxyeicosatrienoic acids (EETs) and leads to salt-sensitive hypertension in humans.

METHODS:
Salt sensitivity was assessed in 19 hypertensive subjects using an inpatient salt loading and depletion protocol. Isolevuglandin-adduct accumulation in APCs was analyzed using flow cytometry. Gene expression in APCs was analyzed using cellular indexing of transcriptomes and epitopes by sequencing analysis of blood mononuclear cells. Plasma and urine EETs were measured using liquid chromatography–mass spectrometry.

RESULTS:

Baseline isolevuglandin
+
APCs correlated with higher salt-sensitivity index. Isolevuglandin
+
APCs significantly decreased from salt loading to depletion with an increasing salt-sensitivity index. We observed that human APCs express the epithelial sodium channel δ subunit, SGK1 (salt-sensing kinase serum/glucocorticoid kinase 1), and cytochrome P450 2S1. We found a direct correlation between baseline urinary 14,15 EET and salt-sensitivity index, whereas changes in urinary 14,15 EET negatively correlated with isolevuglandin
+
monocytes from salt loading to depletion. Coincubation with 14,15 EET inhibited high-salt–induced increase in isolevuglandin
+
APC.

CONCLUSIONS:

Isolevuglandin formation in APCs responds to acute changes in salt intake in salt-sensitive but not salt-resistant people with hypertension, and this may be regulated by renal 14,15 EET. Baseline levels of isolevuglandin
+
APCs or urinary 14,15 EET may provide diagnostic tools for salt sensitivity without a protocol of salt loading.
Seeing a Talking Face Matters: Gaze Behavior and the Auditory–Visual Speech Benefit in Adults' Cortical Tracking of Infant-directed Speech
Authors: Tan Sok Hui Jessica , Kalashnikova Marina , Di Liberto Giovanni M. , Crosse Michael J. , Burnham Denis 
Published: September 2023

Abstract
In face-to-face conversations, listeners gather visual speech information from a speaker's talking face that enhances their perception of the incoming auditory speech signal. This auditory–visual (AV) speech benefit is evident even in quiet environments but is stronger in situations that require greater listening effort such as when the speech signal itself deviates from listeners' expectations. One example is infant-directed speech (IDS) presented to adults. IDS has exaggerated acoustic properties that are easily discriminable from adult-directed speech (ADS). Although IDS is a speech register that adults typically use with infants, no previous neurophysiological study has directly examined whether adult listeners process IDS differently from ADS. To address this, the current study simultaneously recorded EEG and eye-tracking data from adult participants as they were presented with auditory-only (AO), visual-only, and AV recordings of IDS and ADS. Eye-tracking data were recorded because looking behavior to the speaker's eyes and mouth modulates the extent of AV speech benefit experienced. Analyses of cortical tracking accuracy revealed that cortical tracking of the speech envelope was significant in AO and AV modalities for IDS and ADS. However, the AV speech benefit [i.e., AV &gt; (A + V)] was only present for IDS trials. Gaze behavior analyses indicated differences in looking behavior during IDS and ADS trials. Surprisingly, looking behavior to the speaker's eyes and mouth was not correlated with cortical tracking accuracy. Additional exploratory analyses indicated that attention to the whole display was negatively correlated with cortical tracking accuracy of AO and visual-only trials in IDS. Our results underscore the nuances involved in the relationship between neurophysiological AV speech benefit and looking behavior.
Audiotactile Stimulation Can Improve Syllable Discrimination through Multisensory Integration in the Theta Frequency Band
Authors: Guilleminot Pierre , Graef Cosima , Butters Emilia , Reichenbach Tobias 
Published: September 2023

Abstract
Syllables are an essential building block of speech. We recently showed that tactile stimuli linked to the perceptual centers of syllables in continuous speech can improve speech comprehension. The rate of syllables lies in the theta frequency range, between 4 and 8 Hz, and the behavioral effect appears linked to multisensory integration in this frequency band. Because this neural activity may be oscillatory, we hypothesized that a behavioral effect may also occur not only while but also after this activity has been evoked or entrained through vibrotactile pulses. Here, we show that audiotactile integration regarding the perception of single syllables, both on the neural and on the behavioral level, is consistent with this hypothesis. We first stimulated participants with a series of vibrotactile pulses and then presented them with a syllable in background noise. We show that, at a delay of 200 msec after the last vibrotactile pulse, audiotactile integration still occurred in the theta band and syllable discrimination was enhanced. Moreover, the dependence of both the neural multisensory integration as well as of the behavioral discrimination on the delay of the audio signal with respect to the last tactile pulse was consistent with a damped oscillation. In addition, the multisensory gain is correlated with the syllable discrimination score. Our results therefore evidence the role of the theta band in audiotactile integration and provide evidence that these effects may involve oscillatory activity that still persists after the tactile stimulation.
Swish Floats: An Inexpensive Neutrally Buoyant Float to Monitor Dispersion in Coastal Seas
Authors: Stevens Samuel W. , Pawlowicz Rich 
Published: September 2023

Abstract
Neutrally buoyant floats have been widely used to measure flows in the ocean, but deploying them in large numbers can be costly and impractical. This is particularly true near coastlines due to the elevated risk of instrument grounding or vessel collisions, resulting in a lack of subsurface Lagrangian measurements in coastal regions. Here, we describe an inexpensive neutrally buoyant satellite-tracked float (named “Swallow-ish,” or “Swish” floats) that has been designed and tested as a cost-effective strategy to measure subsurface dispersion in coastal areas on time scales up to a month. These autonomous instruments are inexpensive, constructed at a material cost of CAD $300 per unit; lightweight, with a mass of 5.4 kg; isopycnal; and constructed from commercially available components, using recently available global navigation satellite system technology to provide the user with a point-to-point measure of subsurface transport. We describe the float design, ballasting techniques, and the governing equations that determine their behavior. Further, through 29 deployments in two coastal seas, we calculate an uncertainty budget and determine a ballasting error of ±1.6 g, corresponding to a local depth targeting error of 16–30 m, analyze the float resurfacing data to calculate subsurface dispersion coefficients, and examine the float depth records to quantify the local internal wave field. Finally, we evaluate surface dispersion using the postresurfacing trajectories. Our findings indicate that Swish floats offer a cost-effective alternative for Lagrangian measurements of subsurface flows in coastal regions.
Models for forecasting the traffic flow within the city of Ljubljana
Authors: Petelin Gašper , Hribar Rok , Papa Gregor 
Published: September 2023

AbstractEfficient traffic management is essential in modern urban areas. The development of intelligent traffic flow prediction systems can help to reduce travel times and maximize road capacity utilization. However, accurately modeling complex spatiotemporal dependencies can be a difficult task, especially when real-time data collection is not possible. This study aims to tackle this challenge by proposing a solution that incorporates extensive feature engineering to combine historical traffic patterns with covariates such as weather data and public holidays. The proposed approach is assessed using a new real-world data set of traffic patterns collected in Ljubljana, Slovenia. The constructed models are evaluated for their accuracy and hyperparameter sensitivity, providing insights into their performance. By providing practical solutions for real-world scenarios, the proposed approach offers an effective means to improve traffic flow prediction without relying on real-time data.
Micro-mobility and road safety: why do e-scooter riders use the sidewalk? Evidence from a German field study
Authors: Anke Juliane , Ringhand Madlen , Petzoldt Tibor , Gehlert Tina 
Published: September 2023

Abstract
Objectives
Since their introduction in 2019, the use of e-scooters has become widespread in Germany. Concerns about road safety, especially pedestrian safety, have arisen as the popularity of micro-mobility has grown. In light of this context, the present study investigates which types of road infrastructure e-scooter riders use, with a focus on riding on the sidewalk, which is not permitted in Germany. We considered the following infrastructures: (1) off-road bike lane (+ sidewalk and road), (2) on-road bike lane (+ sidewalk and road), and (3) road (+ sidewalk).

Methods
Observations at six sites (recording 738 e-scooter riders) and on-site surveys (involving responses from 129 e-scooter riders) were conducted in two German cities in August 2020 and September 2020.

Results
Self-reported sidewalk riding was not found to be linked directly to a lack of rule knowledge, a preference for this type of infrastructure, or perceived safety. Observations indicated that using the sidewalk might be related to situational components, such as comfort or convenience, comprising up to 40% of instances of sidewalk riding.

Conclusion
Considering the comfort and convenience factor of sites when building or improving cycling infrastructure can help keep e-scooter riders from riding on the sidewalk.
Bone marrow mesenchymal stem cellsderived exosomes stabilize atherosclerosis through inhibiting pyroptosis
Authors: Bai Zhibin , Hu Haolin , Hu Fangfang , Ji Jiajie , Ji Zhenling 
Published: September 2023

Abstract
Objectives
This study aimed to determine the effects of bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (BMSC-EXO) on atherosclerosis (AS), and its related underlying mechanisms.

Methods
Exosomes were isolated from mouse BMSCs, and identified by transmission electron microscopy (TEM), Nanosight (NTA), and western blot. A mouse AS model was established, and exosomes were injected into the tail vein. Total cholesterol (TC) and triglycerides (TG) were detected using their corresponding assay kits. The contents of IL-1β and IL-18 in serum were detected by ELISA. The mRNA and protein expression levels of GSDMD, Caspase1, and NLRP3 were detected by qRT-PCR and Western blot. Finally, aortic tissues in the Model and BMSC-EXO groups were sent for sequencing.

Results
TEM, NTA, and western blot indicated successful isolation of exosomes. Compared with the control group, the TC, TG contents, IL-1β and IL-18 concentrations of the mice in the Model group were significantly increased; nonetheless, were significantly lower after injected with BMSC-EXO than those in the Model group (p < 0.05). Compared with the control group, the expressions of NLRP3, caspase-1 and GSDMD were significantly up-regulated in the Model group (p < 0.05), while the expressions of NLRP3, caspase-1, and GSDMD were significantly down-regulated by BMSC-EXO. By sequencing, a total of 3852 DEGs were identified between the Model and BMSC-EXO group and were significantly enriched in various biological processes and pathways related to mitochondrial function, metabolism, inflammation, and immune response.

Conclusion
AS can induce pyroptosis, and BMSC-EXO can reduce inflammation and alleviate the progression of AS by inhibiting NLRP3/Caspase-1/GSDMD in the pyroptosis pathway.
Etiology and clinical features of infection-associated plastic bronchitis in children
Authors: Huang Feng , Gu Wenjing , Diwu Jianfeng , Zhang Xinxing , He Yanyu , Zhang Youjian , Chen Zhengrong , Huang Li , Wang Meijuan , Dong Heting , Wang Shanshan , Wang Yuqing , Zhu Canhong , Hao Chuangli 
Published: September 2023

Abstract
Objective
To investigate the etiological characteristics of plastic bronchitis (PB) caused by pulmonary infections in children and to identify any differences in the clinical features of PB cases caused by different pathogens.

Method
We collected data on children diagnosed with PB and admitted to the Respiratory Department at Soochow University Children’s Hospital between July 2021 and March 2023 utilizing electronic bronchoscopy. We analyzed clinical characteristics and the species of pathogens causing the illness in these children.

Result
A total of 45 children were enrolled. The main clinical symptoms observed were cough (100%), fever (80%), shortness of breath (28.9%), and wheezing (20.0%). Pathogens were identified in 38 (84.4%) patients. Mycoplasma pneumoniae (MP) had the highest detection rate at 53.3%, followed by the Boca virus at 26.7%. MP-induced PB typically occurs in older children with an average age of 7.46 ± 2.36 years, with the main symptoms including high fever (85.7%) and local hyporespiration (42.9%). In contrast, Boca virus-induced PB tends to occur in younger children, with the main symptoms of moderate fever (54.5%), and wheezing (54.5%). The MP group exhibited a higher incidence of both internal and external pulmonary complications, including pleural effusion (42.9%), elevated aspartate aminotransferase (52.4%), lactic dehydrogenase (76.2%), and D-D dimer (90.5%). Conversely, the Boca virus group primarily showed pulmonary imaging of atelectasis (81.8%), with no pleural effusion. The average number of bronchoscopic interventions in the MP group was 2.24 ± 0.62, which was significantly higher than that required in the Boca virus group (1.55 ± 0.52). During the second bronchoscopy, 57.1% of children in the MP group still had visible mucus plugs, while none were observed in the Boca virus group.

Conclusion
MP and Boca virus are the primary pathogens responsible for PB among children. The clinical manifestations of PB typically vary significantly based on the pathogen causing the condition.
Timing of gestational diabetes diagnosis, gestational weight gains and offspring growth trajectory: a prospective birth cohort study
Authors: Yin Xiao-guang , Wang Peng , Zhou Mei-ting , Li De-qin , Tao Rui-xue , Tao Fang-biao , Wang Yang , Zhu Peng 
Published: September 2023

Abstract
Background
The evidence on the associations of the timing of maternal gestational diabetes mellitus (GDM) with the comprehensive growth trajectory from perinatal to early childhood in offspring is limited. The potential mechanism remains elusive. Our aim is to estimate the associations of the timing of GDM diagnosis and gestational weight gains (GWG) with the growth trajectory of children from perinatal to early childhood.

Methods
A total of 7609 participants are included from the Maternal & Infants Health in Hefei cohort study. Primary predictors were the timing of maternal GDM diagnosis and GWG during pregnancy. The main outcomes included fetal ultrasonic measurements, birth size as well as BMI peak indicators during infancy within 48 months.

Results
GDM diagnosed before 26 weeks was associated with increased risks of overgrowth for fetal abdominal circumference (OR 1.19, 95% CI 1.04–1.36) and birth weight (OR 1.51, 95% CI 1.19–1.91) when compared with unexposed. GDM diagnosis < 26 weeks was related to the higher BMI peak (β 0.16, 95%CI 0.03–0.28) within 48 months. The significantly additive impacts of maternal early GDM diagnosis and excessive gestational weight gains (EGWG) on offspring overgrowth were observed. Women in GDM < 26 weeks with early EGWG group had higher levels of hsCRP compared with GDM > 26 weeks (P < 0.001).

Conclusions
Exposure to maternal GDM diagnosed before 26 weeks with early EGWG could lead to shifts and/or disruptions from the typical growth trajectory from perinatal to early childhood in offspring.
Development and initial qualitative evaluation of a novel school-based nutrition intervention – COOKKIT (Cooking Kit for Kids)
Authors: Pini Simon , Goodman William , Raby Elizabeth , McGinley Chris , Perez-Cornago Aurora , Johnson Fiona , Beeken Rebecca J. 
Published: September 2023

Abstract
Background
Excess weight and an unhealthy diet are risk factors for many cancers, and in high income countries, both are more prevalent among low income families. Dietary interventions targeting primary-school aged children (under 11) can improve healthy eating behaviours, but most are not designed to support the translation of skills learnt in the classroom to the home setting. This paper assessed attitudes and approaches to cooking and eating at home, and the potential to enhance engagement in healthy eating through the COOKKIT intervention.

Methods
COOKKIT is an intervention to deliver weekly cooking classes and supportive materials for low-income families to maintain healthy eating at home. Preliminary qualitative interviews were conducted with teachers and parent–child dyads from a range of primary schools in the UK to explore attitudes, barriers and facilitators for healthy eating and inform the development of COOKKIT. Following implementation, ten children (8–9 y/o) participated in post-intervention focus groups, alongside interviews with teaching staff and parents.

Results
Thematic analysis identified five themes under which to discuss the children’s experience of food, cooking and the impact of COOKKIT: Involving children in planning and buying food for the family; Engaging children in preparing meals at home; Trying to eat healthy meals together in the midst of busy lives; Role-modelling; and Balancing practicalities, information and engagement when delivering cooking classes.

Conclusions
Results suggest COOKKIT provides engaging and easy to follow in-school resources for children and school staff with take-home kits facilitating continued engagement and reinforcing lessons learned in the home environment. Importantly, participants highlighted the combination of healthy eating information, applied practical skills and low costs could support families to continue following the COOKKIT advice beyond the intervention, suggesting further evaluation of COOKKIT is warranted.