bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex differences in iron status during military training: a prospective cohort study of longitudinal changes and associations with endurance performance and musculoskeletal outcomes
Authors: O’Leary Thomas J. , Jackson Sarah , Izard Rachel M. , Walsh Neil P. , Coombs Charlotte V. , Carswell Alexander T. , Oliver Samuel J. , Tang Jonathan C. Y. , Fraser William D. , Greeves Julie P. 
Published: September 2023

AbstractThis study investigated sex differences in Fe status, and associations between Fe status and endurance and musculoskeletal outcomes, in military training. In total, 2277 British Army trainees (581 women) participated. Fe markers and endurance performance (2·4 km run) were measured at the start (week 1) and end (week 13) of training. Whole-body areal body mineral density (aBMD) and markers of bone metabolism were measured at week 1. Injuries during training were recorded. Training decreased Hb in men and women (mean change (–0·1 (95 % CI –0·2, –0·0) and –0·7 (95 % CI –0·9, –0·6) g/dl, both P < 0·001) but more so in women (P < 0·001). Ferritin decreased in men and women (–27 (95 % CI –28, –23) and –5 (95 % CI –8, –1) µg/l, both P ≤ 0·001) but more so in men (P < 0·001). Soluble transferrin receptor increased in men and women (2·9 (95 % CI 2·3, 3·6) and 3·8 (95 % CI 2·7, 4·9) nmol/l, both P < 0·001), with no difference between sexes (P = 0·872). Erythrocyte distribution width increased in men (0·3 (95 % CI 0·2, 0·4)%, P < 0·001) but not in women (0·1 (95 % CI –0·1, 0·2)%, P = 0·956). Mean corpuscular volume decreased in men (–1·5 (95 % CI –1·8, –1·1) fL, P < 0·001) but not in women (0·4 (95 % CI –0·4, 1·3) fL, P = 0·087). Lower ferritin was associated with slower 2·4 km run time (P = 0·018), sustaining a lower limb overuse injury (P = 0·048), lower aBMD (P = 0·021) and higher beta C-telopeptide cross-links of type 1 collagen and procollagen type 1 N-terminal propeptide (both P < 0·001) controlling for sex. Improving Fe stores before training may protect Hb in women and improve endurance and protect against injury.
The Fiction of the Seven Letters in the Apocalypse: Representing Heavenly Authority in the Shadow of Paul
Authors: Frankfurter David 
Published: September 2023

AbstractWhile scholars have traditionally taken Revelation’s “letters to the seven churches” (Rev 2–3) as documentation for the experiences of the Christ-movement in those cities, this article argues that the letters amount to a fictional device—that the Apocalypse appropriates epistolary forms in response to the increasing authority of early Pauline collections among the late first-century Asia Minor Christ-movements. With its divine epistolary authority and heavenly sevenfold “collection,” the Apocalypse attempts to exceed and denigrate Pauline authority in the Christ-movement, and it elevates a Jewish Christ-devotion based in priestly apocalyptic traditions. In the end, we can see John of Patmos both as a competitor to the Pauline tradition and as a witness to the earliest circulation of Pauline collections.
Mothers’ and fathers’ infant-directed speech have similar acoustic properties, but these are not associated with direct or indirect measures of word comprehension in 8-month-old infants
Authors: ROSSLUND Audun , HAGELUND Silje , MAYOR Julien , KARTUSHINA Natalia 
Published: September 2023

AbstractPrevious research on infant-directed speech (IDS) and its role in infants’ language development has largely focused on mothers, with fathers being investigated scarcely. Here we examine the acoustics of IDS as compared to adult-directed speech (ADS) in Norwegian mothers and fathers to 8-month-old infants, and whether these relate to direct (eye-tracking) and indirect (parental report) measures of infants’ word comprehension. Forty-five parent-infant dyads participated in the study. Parents (24 mothers, 21 fathers) were recorded reading a picture book to their infant (IDS), and to an experimenter (ADS), ensuring identical linguistic context across speakers and registers. Results showed that both mothers’ and fathers’ IDS had exaggerated prosody, expanded vowel spaces, as well as more variable and less distinct vowels. We found no evidence that acoustic features of parents’ speech were associated with infants’ word comprehension. Potential reasons for the lack of such a relationship are discussed.
On the zeroes of hypergraph independence polynomials
Authors: Galvin David , McKinley Gwen , Perkins Will , Sarantis Michail , Tetali Prasad 
Published: September 2023

AbstractWe study the locations of complex zeroes of independence polynomials of bounded-degree hypergraphs. For graphs, this is a long-studied subject with applications to statistical physics, algorithms, and combinatorics. Results on zero-free regions for bounded-degree graphs include Shearer’s result on the optimal zero-free disc, along with several recent results on other zero-free regions. Much less is known for hypergraphs. We make some steps towards an understanding of zero-free regions for bounded-degree hypergaphs by proving that all hypergraphs of maximum degree
$\Delta$
have a zero-free disc almost as large as the optimal disc for graphs of maximum degree
$\Delta$
established by Shearer (of radius
$\sim 1/(e \Delta )$
). Up to logarithmic factors in
$\Delta$
this is optimal, even for hypergraphs with all edge sizes strictly greater than
$2$
. We conjecture that for
$k\ge 3$
,
$k$
-uniform linear hypergraphs have a much larger zero-free disc of radius
$\Omega (\Delta ^{- \frac{1}{k-1}} )$
. We establish this in the case of linear hypertrees.
Thermal Conductivity of Hydrogen at High Pressure and High Temperature: Implications to Giant Planets
Authors: Shieh Sean R. , Hsieh Wen‐Pin , Tsao Yi‐Chih 
Published: September 2023

AbstractHydrogen (H2) is the most abundant constituent in giant planets, but its thermal conductivity Λ under extreme pressure‐temperature (P‐T) conditions remains largely unknown. Here we report the Λ of H2 from ambient to 60.2 GPa at 300 K and from 300 to 773 K at 2.1 GPa. At 300 K, the Λ of liquid H2 fluctuates at ∼0.7–1.1 W m−1 K−1. Upon crystallization to H2‐I phase, the Λ jumps to 5.5 W m−1 K−1 at 7.2 GPa, and monotonically increases with pressure to ∼27 W m−1 K−1 at 60.2 GPa. Upon heating, the Λ of liquid H2 at 2.1 GPa scales with T0.68. Moreover, the density (ρ)‐dependent compressional sound velocity (Vp) of liquid and solid H2 derived from Brillouin frequency data both follow the Birch's law. Besides the novel insights into the physics of thermal transport in H2 under extreme conditions, our results significantly advance the modeling of Λ‐Vp‐ρ relationship in a planet with H2.
More Frequent and Persistent Heatwaves Due To Increased Temperature Skewness Projected by a High‐Resolution Earth System Model
Authors: Gao Yang , Wu Yubing , Guo Xiuwen , Kou Wenbin , Zhang Shaoqing , Leung L. Ruby , Chen Xiaodong , Lu Jian , Diffenbaugh Noah S. , Horton Daniel E. , Yao Xiaohong , Gao Huiwang , Wu Lixin 
Published: September 2023

AbstractHeatwaves are strongly associated with temperature distributions, but the mechanisms by which distributions are influenced by climate change remains unclear. Comparing simulations from a high‐spatial resolution Community Earth System Model (CESM‐HR) with those from low‐resolution models, we identify substantial improvements by CESM‐HR in reproducing observed Northern Hemisphere summer temperature skewness, as well as the frequency, intensity, persistence, and total heatwave days. Temperature skewness is strongly linked to land‐atmosphere interactions and atmospheric circulation. Under global warming projections, some regions exhibit enhanced temperature skewness, along with more frequent and persistent heatwaves of greater intensity. We find that in energy‐limited regimes, such as India, negative skewness in latent heat flux facilitates large positive skewness in sensible heat flux, which modulates near‐surface air temperatures. Skewness differences of latent and sensible heat fluxes are amplified under global warming, increasing the temperature skewness. We find that this contrasting flux mechanism is active in several heatwave‐prone regions.
Characterizing temporal trends in populations exposed to aircraft noise around U.S. airports: 1995–2015
Authors: Nguyen Daniel D. , Levy Jonathan I. , Kim Chanmin , Lane Kevin J. , Simon Matthew C. , Hart Jaime E. , Whitsel Eric A. , VoPham Trang , Malwitz Andrew , Peters Junenette L. 
Published: September 2023

Abstract
Background
Aircraft noise is a key concern for communities surrounding airports, with increasing evidence for health effects and inequitable distributions of exposure. However, there have been limited national-scale assessments of aircraft noise exposure over time and across noise metrics, limiting evaluation of population exposure patterns.

Objective
We evaluated national-scale temporal trends in aviation noise exposure by airport characteristics and across racial/ethnic populations in the U.S.

Methods
Noise contours were modeled for 90 U.S. airports in 5-year intervals between 1995 and 2015 using the Federal Aviation Administration’s Aviation Environmental Design Tool. We utilized linear fixed effects models to estimate changes in noise exposure areas for day-night average sound levels (DNL) of 45, 65, and a nighttime equivalent sound level (Lnight) of 45 A-weighted decibels (dB[A]). We used group-based trajectory modeling to identify distinct groups of airports sharing underlying characteristics. We overlaid noise contours and Census tract data from the U.S. Census Bureau and American Community Surveys for 2000 to 2015 to estimate exposure changes overall and by race/ethnicity.

Results
National-scale analyses showed non-monotonic trends in mean exposed areas that peaked in 2000, followed by a 37% decrease from 2005 to 2010 and a subsequent increase in 2015. We identified four distinct trajectory groups of airports sharing latent characteristics related to size and activity patterns. Those populations identifying as minority (e.g., Hispanic/Latino, Black/African American, Asian) experienced higher proportions of exposure relative to their subgroup populations compared to non-Hispanic or White populations across all years, indicating ethnic and racial disparities in airport noise exposure that persist over time.

Significance
Overall, these data identified differential exposure trends across airports and subpopulations, helping to identify vulnerable communities for aviation noise in the U.S.

Impact statement
We conducted a descriptive analysis of temporal trends in aviation noise exposure in the U.S. at a national level. Using data from 90 U.S. airports over a span of two decades, we characterized the noise exposure trends overall and by airport characteristics, while estimating the numbers of exposed by population demographics to help identify the impact on vulnerable communities who may bear the burden of aircraft noise exposure.
Dynamic single-cell mapping unveils Epstein‒Barr virus-imprinted T-cell exhaustion and on-treatment response
Authors: Qiu Miao-Zhen , Wang Chaoye , Wu Zhiying , Zhao Qi , Zhao Zhibin , Huang Chun-Yu , Wu Wenwei , Yang Li-Qiong , Zhou Zhi-Wei , Zheng Yu , Pan Hong-Ming , Liu Zexian , Zeng Zhao-Lei , Luo Hui-Yan , Wang Feng , Wang Feng-Hua , Yang Si-Yu , Huang Meng-Xing , Lian Zhexiong , Zhang Haiyan , Xu Rui-Hua 
Published: September 2023

AbstractEpstein‒Barr virus (EBV)-associated gastric cancer (GC) manifests an intriguing immunotherapy response. However, the cellular basis for EBV-imprinted tumour immunity and on-treatment response remains undefined. This study aimed to finely characterize the dynamic tumour immune contexture of human EBV (+) GC treated with immunochemotherapy by longitudinal scRNA-seq and paired scTCR/BCR-seq. EBV (+) GC exhibits an inflamed-immune phenotype with increased T-cell and B-cell infiltration. Immunochemotherapy triggers clonal revival and reinvigoration of effector T cells which step to determine treatment response. Typically, an antigen-specific ISG-15+CD8+ T-cell population is highly enriched in EBV (+) GC patients, which represents a transitory exhaustion state. Importantly, baseline intratumoural ISG-15+CD8+ T cells predict immunotherapy responsiveness among GC patients. Re-emerged clonotypes of pre-existing ISG-15+CD8+ T cells could be found after treatment, which gives rise to a CXCL13-expressing effector population in responsive EBV (+) tumours. However, LAG-3 retention may render the ISG-15+CD8+ T cells into a terminal exhaustion state in non-responsive EBV (+) tumours. In accordance, anti-LAG-3 therapy could effectively reduce tumour burden in refractory EBV (+) GC patients. Our results delineate a distinct implication of EBV-imprinted on-treatment T-cell immunity in GC, which could be leveraged to optimize the rational design of precision immunotherapy.
Regulation of cellular cholesterol distribution via non-vesicular lipid transport at ER-Golgi contact sites
Authors: Naito Tomoki , Yang Haoning , Koh Dylan Hong Zheng , Mahajan Divyanshu , Lu Lei , Saheki Yasunori 
Published: September 2023

AbstractAbnormal distribution of cellular cholesterol is associated with numerous diseases, including cardiovascular and neurodegenerative diseases. Regulated transport of cholesterol is critical for maintaining its proper distribution in the cell, yet the underlying mechanisms remain unclear. Here, we show that lipid transfer proteins, namely ORP9, OSBP, and GRAMD1s/Asters (GRAMD1a/GRAMD1b/GRAMD1c), control non-vesicular cholesterol transport at points of contact between the ER and the trans-Golgi network (TGN), thereby maintaining cellular cholesterol distribution. ORP9 localizes to the TGN via interaction between its tandem α-helices and ORP10/ORP11. ORP9 extracts PI4P from the TGN to prevent its overaccumulation and suppresses OSBP-mediated PI4P-driven cholesterol transport to the Golgi. By contrast, GRAMD1s transport excess cholesterol from the Golgi to the ER, thereby preventing its build-up. Cells lacking ORP9 exhibit accumulation of cholesterol at the Golgi, which is further enhanced by additional depletion of GRAMD1s with major accumulation in the plasma membrane. This is accompanied by chronic activation of the SREBP-2 signalling pathway. Our findings reveal the importance of regulated lipid transport at ER-Golgi contacts for maintaining cellular cholesterol distribution and homeostasis.
Engineering the temporal dynamics of all-optical switching with fast and slow materials
Authors: Saha Soham , Diroll Benjamin T. , Ozlu Mustafa Goksu , Chowdhury Sarah N. , Peana Samuel , Kudyshev Zhaxylyk , Schaller Richard D. , Jacob Zubin , Shalaev Vladimir M. , Kildishev Alexander V. , Boltasseva Alexandra 
Published: September 2023

AbstractAll-optical switches control the amplitude, phase, and polarization of light using optical control pulses. They can operate at ultrafast timescales – essential for technology-driven applications like optical computing, and fundamental studies like time-reflection. Conventional all-optical switches have a fixed switching time, but this work demonstrates that the response-time can be controlled by selectively controlling the light-matter-interaction in so-called fast and slow materials. The bi-material switch has a nanosecond response when the probe interacts strongly with titanium nitride near its epsilon-near-zero (ENZ) wavelength. The response-time speeds up over two orders of magnitude with increasing probe-wavelength, as light’s interaction with the faster Aluminum-doped zinc oxide (AZO) increases, eventually reaching the picosecond-scale near AZO’s ENZ-regime. This scheme provides several additional degrees of freedom for switching time control, such as probe-polarization and incident angle, and the pump-wavelength. This approach could lead to new functionalities within key applications in multiband transmission, optical computing, and nonlinear optics.
Ultrasound trapping and navigation of microrobots in the mouse brain vasculature
Authors: Del Campo Fonseca Alexia , Glück Chaim , Droux Jeanne , Ferry Yann , Frei Carole , Wegener Susanne , Weber Bruno , El Amki Mohamad , Ahmed Daniel 
Published: September 2023

AbstractThe intricate and delicate anatomy of the brain poses significant challenges for the treatment of cerebrovascular and neurodegenerative diseases. Thus, precise local drug delivery in hard-to-reach brain regions remains an urgent medical need. Microrobots offer potential solutions; however, their functionality in the brain remains restricted by limited imaging capabilities and complications within blood vessels, such as high blood flows, osmotic pressures, and cellular responses. Here, we introduce ultrasound-activated microrobots for in vivo navigation in brain vasculature. Our microrobots consist of lipid-shelled microbubbles that autonomously aggregate and propel under ultrasound irradiation. We investigate their capacities in vitro within microfluidic-based vasculatures and in vivo within vessels of a living mouse brain. These microrobots self-assemble and execute upstream motion in brain vasculature, achieving velocities up to 1.5 µm/s and moving against blood flows of ~10 mm/s. This work represents a substantial advance towards the therapeutic application of microrobots within the complex brain vasculature.
Endothelial Sp1/Sp3 are essential to the effect of captopril on blood pressure in male mice
Authors: Lu Hanlin , Jiang Xiuxin , He Lifan , Ji Xuyang , Li Xinyun , Liu Shaozhuang , Sun Yuanyuan , Qin Xiaoteng , Xiong Xiwen , Philipsen Sjaak , Xi Bo , Zhang Meng , Yang Jianmin , Zhang Cheng , Zhang Yun , Zhang Wencheng 
Published: September 2023

AbstractEndothelial dysfunction represents a major cardiovascular risk factor for hypertension. Sp1 and Sp3 belong to the specificity protein and Krüppel-like transcription factor families. They are ubiquitously expressed and closely associated with cardiovascular development. We investigate the role of Sp1 and Sp3 in endothelial cells in vivo and evaluate whether captopril, an angiotensin-converting enzyme inhibitor (ACEI), targets Sp1/Sp3 to exert its effects. Inducible endothelial-specific Sp1/Sp3 knockout mice are generated to elucidate their role in endothelial cells. Tamoxifen-induced deletion of endothelial Sp1 and Sp3 in male mice decreases the serum nitrite/nitrate level, impairs endothelium-dependent vasodilation, and causes hypertension and cardiac remodeling. The beneficial actions of captopril are abolished by endothelial-specific deletion of Sp1/Sp3, indicating that they may be targets for ACEIs. Captopril increases Sp1/Sp3 protein levels by recruiting histone deacetylase 1, which elevates deacetylation and suppressed degradation of Sp1/Sp3. Sp1/Sp3 represents innovative therapeutic target for captopril to prevent cardiovascular diseases.
Thermodynamic forces from protein and water govern condensate formation of an intrinsically disordered protein domain
Authors: Mukherjee Saumyak , Schäfer Lars V. 
Published: September 2023

AbstractLiquid-liquid phase separation (LLPS) can drive a multitude of cellular processes by compartmentalizing biological cells via the formation of dense liquid biomolecular condensates, which can function as membraneless organelles. Despite its importance, the molecular-level understanding of the underlying thermodynamics of this process remains incomplete. In this study, we use atomistic molecular dynamics simulations of the low complexity domain (LCD) of human fused in sarcoma (FUS) protein to investigate the contributions of water and protein molecules to the free energy changes that govern LLPS. Both protein and water components are found to have comparably sizeable thermodynamic contributions to the formation of FUS condensates. Moreover, we quantify the counteracting effects of water molecules that are released into the bulk upon condensate formation and the waters retained within the protein droplets. Among the various factors considered, solvation entropy and protein interaction enthalpy are identified as the most important contributions, while solvation enthalpy and protein entropy changes are smaller. These results provide detailed molecular insights on the intricate thermodynamic interplay between protein- and solvation-related forces underlying the formation of biomolecular condensates.
Unit-cell-thick zeolitic imidazolate framework films for membrane application
Authors: Liu Qi , Miao Yurun , Villalobos Luis Francisco , Li Shaoxian , Chi Heng-Yu , Chen Cailing , Vahdat Mohammad Tohidi , Song Shuqing , Babu Deepu J. , Hao Jian , Han Yu , Tsapatsis Michael , Agrawal Kumar Varoon 
Published: September 2023

AbstractZeolitic imidazolate frameworks (ZIFs) are a subset of metal–organic frameworks with more than 200 characterized crystalline and amorphous networks made of divalent transition metal centres (for example, Zn2+ and Co2+) linked by imidazolate linkers. ZIF thin films have been intensively pursued, motivated by the desire to prepare membranes for selective gas and liquid separations. To achieve membranes with high throughput, as in ångström-scale biological channels with nanometre-scale path lengths, ZIF films with the minimum possible thickness—down to just one unit cell—are highly desired. However, the state-of-the-art methods yield membranes where ZIF films have thickness exceeding 50 nm. Here we report a crystallization method from ultradilute precursor mixtures, which exploits registry with the underlying crystalline substrate, yielding (within minutes) crystalline ZIF films with thickness down to that of a single structural building unit (2 nm). The film crystallized on graphene has a rigid aperture made of a six-membered zinc imidazolate coordination ring, enabling high-permselective H2 separation performance. The method reported here will probably accelerate the development of two-dimensional metal–organic framework films for efficient membrane separation.
The efferent connections of the orbitofrontal, posterior parietal, and insular cortex of the rat brain
Authors: Reiten Ingrid , Olsen Grethe M. , Bjaalie Jan G. , Witter Menno P. , Leergaard Trygve B. 
Published: September 2023

AbstractThe orbitofrontal, posterior parietal, and insular cortices are sites of higher-order cognitive processing implicated in a wide range of behaviours, including working memory, attention guiding, decision making, and spatial navigation. To better understand how these regions contribute to such functions, we need detailed knowledge about the underlying structural connectivity. Several tract-tracing studies have investigated specific aspects of orbitofrontal, posterior parietal and insular connectivity, but a digital resource for studying the cortical and subcortical projections from these areas in detail is not available. We here present a comprehensive collection of brightfield and fluorescence microscopic images of serial coronal sections from 49 rat brain tract-tracing experiments, in which discrete injections of the anterograde tracers biotinylated dextran amine and/or Phaseolus vulgaris leucoagglutinin were placed in the orbitofrontal, parietal, or insular cortex. The images are spatially registered to the Waxholm Space Rat brain atlas. The image collection, with corresponding reference atlas maps, is suitable as a reference framework for investigating the brain-wide efferent connectivity of these cortical association areas.