bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multifaceted provider-centred intervention versus usual care to improve the recognition and diagnosis of depression in primary health care: a hybrid study
Authors: Nogueras Eva Vanesa , Cantero Nazaret , Macías María , Morales-Asencio José Miguel , García-Herrera Pérez-Bryan José María , Hurtado María M. 
Published: July 2023

AbstractBackground:The aim of this study was to evaluate the impact of a multifaceted intervention to implement an adapted guideline for the management of depression in primary health care.Methods:A hybrid trial was carried out to determine the effect of a multicomponent provider-centred intervention to improve the detection and diagnosis of depression in primary care, as part of the guideline implementation process, and to collect information about barriers and facilitators in a real-world context. Before the multicomponent intervention, a descriptive cross-sectional study was performed to assess the population prevalence of depression in the participating health centres and to detect possible differences. Subsequently, a quasi-experimental two-phase study was carried out with a concurrent control group to assess the impact of the multicomponent intervention on the main outcomes (detection of depression, evaluation of its severity and the use of structured methods to support the diagnosis).Results:Nine-hundred seventy-four patients took part in the first phase. According to their clinical records, the prevalence of depression ranged from 7.2% to 7.9%, and there were no significant differences between the health centres scheduled to receive the intervention and those in the control group. In the experimental phase, 797 randomly selected participants received the multicomponent intervention. Adjusted multivariable analysis performed before the implementation revealed no significant differences in depression between the experimental and control groups. However, after the intervention, modest but significant differences were observed, which persisted at 1 year after the intervention.Conclusions:A multicomponent intervention for the implementation of a clinical guideline for the management of depression in primary care produced improvements in the identification of depression and in the degree of severity recorded.
The effects of cereal rye cover crop seeding rate, termination timing, and herbicide inputs on weed control and soybean yield
Authors: Essman Alyssa I. , Loux Mark M. , Lindsey Alexander J. , Dobbels Anthony F. 
Published: July 2023

AbstractGrowers have been experimenting with cover crop termination timings to maximize weed suppression and potentially reduce herbicide inputs in soybean [Glycine max (L.) Merr.]. A field study was replicated three times from 2018 through 2021 in South Charleston, OH, to evaluate different management strategies involving a cereal rye (Secale cereale L.) cover crop. The objectives were to determine the effects of cereal rye seeding rate (0, 50, and 100 kg ha−1), management program (preplant, postplant, and delayed), and soybean residual herbicide (flumioxazin + chlorimuron ethyl and no herbicide) on cover crop, weed, and soybean parameters. The preplant program consisted of cereal rye terminated 7 d before planting (DBP) + a postemergence application. The postplant program consisted of cereal rye terminated 7 d after planting (DAP) + a postemergence application. In the delayed program, saflufenacil was applied in April and cereal rye was terminated 21 DAP, and there was no postemergence application. Giant foxtail (Setaria faberi Herrm.) density was reduced by the presence of cereal rye, averaged over other factors, regardless of seeding rate. Cereal rye seeding rate did not affect giant ragweed (Ambrosia trifida L.) density. The delayed management program was generally associated with the lowest weed density, but weed density was often similar in the postplant program. Setaria faberi density was lower in treatments that included a residual herbicide. Residual soybean herbicide use did not affect density of A. trifida. Terminating cereal rye after soybean planting resulted in increased soybean yield in 2019 and reduced yield in 2020, compared with preplant rye termination. These data suggest that adjusting the cereal rye management program may have a greater effect on weed suppression than adjustments to seeding rate. Delaying termination of cereal rye can aid in the suppression of weeds, but a comprehensive herbicide program was necessary to provide adequate (>85%) weed control.
Views of RNAi approaches for weed management in turfgrass systems
Authors: Ethridge Sandra R. , Grieger Khara , Locke Anna M. , Everman Wesley J. , Jordan David L. , Leon Ramon G. 
Published: July 2023

AbstractPublic concern regarding the use of herbicides in urban areas (e.g., golf courses, parks, lawns) is increasing. Thus, there is a need for alternative methods for weed control that are safe for the public, effective against weeds, and yet selective to turfgrass and other desirable species. New molecular tools such as ribonucleic acid interference (RNAi) have the potential to meet all those requirements, but before these technologies can be implemented, it is critical to understand the perceptions of key stakeholders to facilitate adoption as well as regulatory processes. With this in mind, turfgrass system managers, such as golf course superintendents and lawn care providers, were surveyed to gain insight into the perception and potential adoption of RNAi technology for weed management. Based on survey results, turfgrass managers believe that cost of weed management and time spent managing weeds are the main challenges faced in their fields. When considering new weed management tools, survey respondents were most concerned about cost, efficacy, and efficiency of a new product. Survey respondents were also optimistic toward RNAi for weed management and would either use this technology in their own fields or be willing to conduct research to develop RNAi herbicides. Although respondents believed that the general public would have some concerns about this technology, they did not believe this to be the most important factor for them when choosing new weed management tools. The need for new herbicides to balance weed control challenges and public demands is a central factor for turfgrass managers’ willingness to use RNAi-based weed control in turfgrass systems. They believe their clientele will be accepting of RNAi tools, although further research is needed to investigate how a wider range of stakeholders perceive RNAi tools for turfgrass management more broadly.
Dynamics of Suspended Particulate Matter in an Impounded River Under Dry and Wet Weather Conditions
Authors: Huynh T. T. , Kim J. , Kim W. , Hur J. , Ho Q. N. , Bi Q. , Bui T. V. , Kim J. J. , Lee S. D. , Choi Y. Y. , Lee B. J. 
Published: July 2023

AbstractThe dynamics of suspended particulate matter (SPM) and SPM‐associated biological and physicochemical processes in a river vary under dry and wet weather conditions and hydraulic structures, which moves a free‐flowing river to a semi‐lacustrine environment. This study investigated the response of SPM dynamics and riverbed morphodynamics to climate and anthropogenic stressors based on in‐situ observations and biogeochemical analyses of river water and riverbed sediment. The biochemical analyses and flocculation tests demonstrate abundant biopolymers and high flocculation potential of the river water during the pre‐flood period with algal bloom. This might promote SPM deposition and high‐concentration sediment layer (HCSL) formation on the riverbed. The high fractions of clay minerals and organic carbon in the riverbed sediment indicate the deposition of organic‐rich biomineral flocs. Vertical turbidity profiles with “long tails” of high turbidity near the riverbed also confirmed SPM deposition and HCSL formation. However, the highly turbulent flow conditions during the post‐flood period disturbed the tails of high turbidity and homogenized the vertical profiles of turbidity, temperature, and dissolved oxygen. Additionally, terrestrial humic substances were discharged from the watershed during this period, increasing the deflocculation and stabilization of SPM but decreasing deposition on the riverbed, thereby reducing the fractions of clay minerals and organic carbon in the sediment. This study demonstrated the interaction mechanism of SPM dynamics and riverbed morphodynamics with hydrological and biogeochemical processes in an impounded river under dry and wet weather conditions. The findings provide insights into water resource management to deal with climate change and anthropogenic stressors.
Targeting PP2A-dependent autophagy enhances sensitivity to ruxolitinib in JAK2V617F myeloproliferative neoplasms
Authors: Courdy Charly , Platteeuw Loïc , Ducau Charlotte , De Araujo Isabelle , Boet Emeline , Sahal Ambrine , Saland Estelle , Edmond Valérie , Tavitian Suzanne , Bertoli Sarah , Cougoul Pierre , Granat Fanny , Poillet Laura , Marty Caroline , Plo Isabelle , Sarry Jean-Emmanuel , Manenti Stéphane , Mansat-De Mas Véronique , Joffre Carine 
Published: July 2023

AbstractThe Janus kinase 2 (JAK2)-driven myeloproliferative neoplasms (MPNs) are chronic malignancies associated with high-risk complications and suboptimal responses to JAK inhibitors such as ruxolitinib. A better understanding of cellular changes induced by ruxolitinib is required to develop new combinatory therapies to improve treatment efficacy. Here, we demonstrate that ruxolitinib induced autophagy in JAK2V617F cell lines and primary MPN patient cells through the activation of protein phosphatase 2A (PP2A). Inhibition of autophagy or PP2A activity along with ruxolitinib treatment reduced proliferation and increased the death of JAK2V617F cells. Accordingly, proliferation and clonogenic potential of JAK2V617F-driven primary MPN patient cells, but not of normal hematopoietic cells, were markedly impaired by ruxolitinib treatment with autophagy or PP2A inhibitor. Finally, preventing ruxolitinib-induced autophagy with a novel potent autophagy inhibitor Lys05 improved leukemia burden reduction and significantly prolonged the mice’s overall survival compared with ruxolitinib alone. This study demonstrates that PP2A-dependent autophagy mediated by JAK2 activity inhibition contributes to resistance to ruxolitinib. Altogether, our data support that targeting autophagy or its identified regulator PP2A could enhance sensitivity to ruxolitinib of JAK2V617F MPN cells and improve MPN patient care.
Type 2 immunity in the brain and brain borders
Authors: Mamuladze Tornike , Kipnis Jonathan 
Published: July 2023

AbstractRecent research in neuroimmunology has revolutionized our understanding of the intricate interactions between the immune system and the central nervous system (CNS). The CNS, an “immune-privileged organ”, is now known to be intimately connected to the immune system through different cell types and cytokines. While type 2 immune responses have traditionally been associated with allergy and parasitic infections, emerging evidence suggests that these responses also play a crucial role in CNS homeostasis and disease pathogenesis. Type 2 immunity encompasses a delicate interplay among stroma, Th2 cells, innate lymphoid type 2 cells (ILC2s), mast cells, basophils, and the cytokines interleukin (IL)-4, IL-5, IL-13, IL-25, TSLP and IL-33. In this review, we discuss the beneficial and detrimental roles of type 2 immune cells and cytokines in CNS injury and homeostasis, cognition, and diseases such as tumors, Alzheimer’s disease and multiple sclerosis.
Genome-wide association analysis and Mendelian randomization proteomics identify drug targets for heart failure
Authors: Rasooly Danielle , Peloso Gina M. , Pereira Alexandre C. , Dashti Hesam , Giambartolomei Claudia , Wheeler Eleanor , Aung Nay , Ferolito Brian R. , Pietzner Maik , Farber-Eger Eric H. , Wells Quinn Stanton , Kosik Nicole M. , Gaziano Liam , Posner Daniel C. , Bento A. Patrícia , Hui Qin , Liu Chang , Aragam Krishna , Wang Zeyuan , Charest Brian , Huffman Jennifer E. , Wilson Peter W. F. , Phillips Lawrence S. , Whittaker John , Munroe Patricia B. , Petersen Steffen E. , Cho Kelly , Leach Andrew R. , Magariños María Paula , Gaziano John Michael ,   VA Million Veteran Program, Langenberg Claudia , Sun Yan V. , Joseph Jacob , Casas Juan P. 
Published: July 2023

AbstractWe conduct a large-scale meta-analysis of heart failure genome-wide association studies (GWAS) consisting of over 90,000 heart failure cases and more than 1 million control individuals of European ancestry to uncover novel genetic determinants for heart failure. Using the GWAS results and blood protein quantitative loci, we perform Mendelian randomization and colocalization analyses on human proteins to provide putative causal evidence for the role of druggable proteins in the genesis of heart failure. We identify 39 genome-wide significant heart failure risk variants, of which 18 are previously unreported. Using a combination of Mendelian randomization proteomics and genetic cis-only colocalization analyses, we identify 10 additional putatively causal genes for heart failure. Findings from GWAS and Mendelian randomization-proteomics identify seven (CAMK2D, PRKD1, PRKD3, MAPK3, TNFSF12, APOC3 and NAE1) proteins as potential targets for interventions to be used in primary prevention of heart failure.
QBO deepens MJO convection
Authors: Jin Daeho , Kim Daehyun , Son Seok-Woo , Oreopoulos Lazaros 
Published: July 2023

AbstractThe underlying mechanism that couples the Quasi-Biennial Oscillation (QBO) and the Madden-Julian oscillation (MJO) has remained elusive, challenging our understanding of both phenomena. A popular hypothesis about the QBO-MJO connection is that the vertical extent of MJO convection is strongly modulated by the QBO. However, this hypothesis has not been verified observationally. Here we show that the cloud-top pressure and brightness temperature of deep convection and anvil clouds are systematically lower in the easterly QBO (EQBO) winters than in the westerly QBO (WQBO) winters, indicating that the vertical growth of deep convective systems within MJO envelopes is facilitated by the EQBO mean state. Moreover, the deeper clouds during EQBO winters are more effective at reducing longwave radiation escaping to space and thereby enhancing longwave cloud-radiative feedback within MJO envelopes. Our results provide robust observational evidence of the enhanced MJO activity during EQBO winters by mean state changes induced by the QBO.
Nanoconfinement enabled non-covalently decorated MXene membranes for ion-sieving
Authors: Kang Yuan , Hu Ting , Wang Yuqi , He Kaiqiang , Wang Zhuyuan , Hora Yvonne , Zhao Wang , Xu Rongming , Chen Yu , Xie Zongli , Wang Huanting , Gu Qinfen , Zhang Xiwang 
Published: July 2023

AbstractCovalent modification is commonly used to tune the channel size and functionality of 2D membranes. However, common synthesis strategies used to produce such modifications are known to disrupt the structure of the membranes. Herein, we report less intrusive yet equally effective non-covalent modifications on Ti3C2Tx MXene membranes by a solvent treatment, where the channels are robustly decorated by protic solvents via hydrogen bond network. The densely functionalized (-O, -F, -OH) Ti3C2Tx channel allows multiple hydrogen bond establishment and its sub-1-nm size induces a nanoconfinement effect to greatly strengthen these interactions by maintaining solvent-MXene distance and solvent orientation. In sub-1-nm ion sieving and separation, as-decorated membranes exhibit stable ion rejection, and proton-cation (H+/Mn+) selectivity that is up to 50 times and 30 times, respectively, higher than that of pristine membranes. It demonstrates the feasibility of non-covalent methods as a broad modification alternative for nanochannels integrated in energy-, resource- and environment-related applications.
Group size and mating system predict sex differences in vocal fundamental frequency in anthropoid primates
Authors: Aung Toe , Hill Alexander K. , Pfefferle Dana , McLester Edward , Fuller James , Lawrence Jenna M. , Garcia-Nisa Ivan , Kendal Rachel L. , Petersdorf Megan , Higham James P. , Galat Gérard , Lameira Adriano R. , Apicella Coren L. , Barelli Claudia , Glenn Mary E. , Ramos-Fernandez Gabriel , Puts David A. 
Published: July 2023

AbstractVocalizations differ substantially between the sexes in many primates, and low-frequency male vocalizations may be favored by sexual selection because they intimidate rivals and/or attract mates. Sexual dimorphism in fundamental frequency may be more pronounced in species with more intense male mating competition and in those with large group size, where social knowledge is limited and efficient judgment of potential mates and competitors is crucial. These non-mutually exclusive explanations have not been tested simultaneously across primate species. In a sample of vocalizations (n = 1914 recordings) across 37 anthropoid species, we investigated whether fundamental frequency dimorphism evolved in association with increased intensity of mating competition (H1), large group size (H2), multilevel social organization (H3), a trade-off against the intensity of sperm competition (H4), and/or poor acoustic habitats (H5), controlling for phylogeny and body size dimorphism. We show that fundamental frequency dimorphism increased in evolutionary transitions towards larger group size and polygyny. Findings suggest that low-frequency male vocalizations in primates may have been driven by selection to win mating opportunities by avoiding costly fights and may be more important in larger groups, where limited social knowledge affords advantages to rapid assessment of status and threat potential via conspicuous secondary sexual characteristics.
Cell cycle arrest and p53 prevent ON-target megabase-scale rearrangements induced by CRISPR-Cas9
Authors: Cullot G. , Boutin J. , Fayet S. , Prat F. , Rosier J. , Cappellen D. , Lamrissi I. , Pennamen P. , Bouron J. , Amintas S. , Thibault C. , Moranvillier I. , Laharanne E. , Merlio J. P. , Guyonnet-Duperat V. , Blouin J. M. , Richard E. , Dabernat S. , Moreau-Gaudry F. , Bedel A. 
Published: July 2023

AbstractThe CRISPR-Cas9 system has revolutionized our ability to precisely modify the genome and has led to gene editing in clinical applications. Comprehensive analysis of gene editing products at the targeted cut-site has revealed a complex spectrum of outcomes. ON-target genotoxicity is underestimated with standard PCR-based methods and necessitates appropriate and more sensitive detection methods. Here, we present two complementary Fluorescence-Assisted Megabase-scale Rearrangements Detection (FAMReD) systems that enable the detection, quantification, and cell sorting of edited cells with megabase-scale loss of heterozygosity (LOH). These tools reveal rare complex chromosomal rearrangements caused by Cas9-nuclease and show that LOH frequency depends on cell division rate during editing and p53 status. Cell cycle arrest during editing suppresses the occurrence of LOH without compromising editing. These data are confirmed in human stem/progenitor cells, suggesting that clinical trials should consider p53 status and cell proliferation rate during editing to limit this risk by designing safer protocols.
Assembly drives regioselective azide-alkyne cycloaddition reaction
Authors: Jiang Qiaochu , Zhan Wenjun , Liu Xiaoyang , Bai Lin , Wang Manli , Xu Ying , Liang Gaolin 
Published: July 2023

AbstractAzide-alkyne cycloaddition reaction is a very common organic reaction to synthesize nitrogen-containing heterocycles. Once catalyzed by Cu(I) or Ru(II), it turns out to be a click reaction and thus is widely applied in chemical biology for labeling. However, besides their poor regioselectivity towards this reaction, these metal ions are not biologically friendly. Hence, it is an urgent need to develop a metal-free azide–alkyne cycloaddition reaction for biomedical applications. In this work, we found that, in the absence of metal ions, supramolecular self-assembly in an aqueous solution could realize this reaction with excellent regioselectivity. Nap-Phe-Phe-Lys(azido)-OH firstly self-assembled into nanofibers. Then, Nap-Phe-Phe-Gly(alkynyl)-OH at equivalent concentration approached to react with the assembly to yield the cycloaddition product Nap-Phe-Phe-Lys(triazole)-Gly-Phe-Phe-Nap to form nanoribbons. Due to space confinement effect, the product was obtained with excellent regioselectivity. Employing the excellent properties of supramolecular self-assembly, we are applying this strategy to realize more reactions without metal ion catalysis.
A sodium-ion-conducted asymmetric electrolyzer to lower the operation voltage for direct seawater electrolysis
Authors: Shi Hao , Wang Tanyuan , Liu Jianyun , Chen Weiwei , Li Shenzhou , Liang Jiashun , Liu Shuxia , Liu Xuan , Cai Zhao , Wang Chao , Su Dong , Huang Yunhui , Elbaz Lior , Li Qing 
Published: July 2023

AbstractHydrogen produced from neutral seawater electrolysis faces many challenges including high energy consumption, the corrosion/side reactions caused by Cl-, and the blockage of active sites by Ca2+/Mg2+precipitates. Herein, we design a pH-asymmetric electrolyzer with a Na+exchange membrane for direct seawater electrolysis, which can simultaneously prevent Cl-corrosion and Ca2+/Mg2+precipitation and harvest the chemical potentials between the different electrolytes to reduce the required voltage. In-situ Raman spectroscopy and density functional theory calculations reveal that water dissociation can be promoted with a catalyst based on atomically dispersed Pt anchored to Ni-Fe-P nanowires with a reduced energy barrier (by 0.26 eV), thus accelerating the hydrogen evolution kinetics in seawater. Consequently, the asymmetric electrolyzer exhibits current densities of 10 mA cm−2and 100 mA cm−2at voltages of 1.31 V and 1.46 V, respectively. It can also reach 400 mA cm−2at a low voltage of 1.66 V at 80 °C, corresponding to the electricity cost of US$1.36 per kg of H2($0.031/kW h for the electricity bill), lower than the United States Department of Energy 2025 target (US$1.4 per kg of H2).
Realizing long-cycling all-solid-state Li-In||TiS2 batteries using Li6+xMxAs1-xS5I (M=Si, Sn) sulfide solid electrolytes
Authors: Lu Pushun , Xia Yu , Sun Guochen , Wu Dengxu , Wu Siyuan , Yan Wenlin , Zhu Xiang , Lu Jiaze , Niu Quanhai , Shi Shaochen , Sha Zhengju , Chen Liquan , Li Hong , Wu Fan 
Published: July 2023

AbstractInorganic sulfide solid-state electrolytes, especially Li6PS5X (X = Cl, Br, I), are considered viable materials for developing all-solid-state batteries because of their high ionic conductivity and low cost. However, this class of solid-state electrolytes suffers from structural and chemical instability in humid air environments and a lack of compatibility with layered oxide positive electrode active materials. To circumvent these issues, here, we propose Li6+xMxAs1-xS5I (M=Si, Sn) as sulfide solid electrolytes. When the Li6+xSixAs1-xS5I (x = 0.8) is tested in combination with a Li-In negative electrode and Ti2S-based positive electrode at 30 °C and 30 MPa, the Li-ion lab-scale Swagelok cells demonstrate long cycle life of almost 62500 cycles at 2.44 mA cm−2, decent power performance (up to 24.45 mA cm−2) and areal capacity of 9.26 mAh cm−2 at 0.53 mA cm−2.