bluuebunny/crossref_metadata_2025_split huggingface dataset:
An experimental implication of long‐term hot‐wet‐aged carbon fiber/polyether ketone ketone composites: The impact of automated fiber placement process parameters and process‐induced defects
Authors: Sukur Emine Feyza , Elmas Sinem , Eskizeybek Volkan , Sas Hatice S. , Yildiz Mehmet 
Published: May 2023

AbstractDuring the service life of aerospace‐grade composites, process parameters and process‐induced defects may become crucial. Most studies in this field have mainly focused on the relationship between process‐induced defects and mechanical performance. However, the potential impact of process parameters and process‐induced defects on the service life of composites serving under severe service conditions has received little attention. In this work, the effects of hydrothermal conditioning on the mechanical performance of carbon fiber/polyether ketone ketone (CF/PEKK) composites are examined, along with the correlation between automated fiber placement (AFP) process parameters and process‐induced defects. For this, gap and overlap defects integrated CF/PEKK laminates were exposed to a long‐term (90 days) hot‐wet aging environment to simulate the actual service conditions. Defect‐induced composite samples reached saturation point at the end of 30 days with a mass gain of 0.2 wt%. The aging process resulted in an increase in the degree of crystallization by almost 14% without a change in the chemical structure, indicating the postcrystallization of the PEKK matrix. Even though the thermo‐mechanical performance diminished (~25%) with the aging process, storage modulus was slightly affected by process parameters and process‐induced defects. Considering the flexural and shear test results after the aging process, the impact of gap and overlap defects on the service life of AFP composites can be minimized with higher compaction forces (600 N) and lower lay‐up speeds (0.1 m/s).
Fabrication and processing of bacterial cellulose/silver nanowire composites as transparent, conductive, and flexible films for optoelectronic applications
Authors: Gounden Denisha , Pillay Michael N. , Moodley Vashen , Nombona Nolwazi , van Zyl Werner E. 
Published: May 2023

AbstractThis work reports on the engineering and fabrication of transparent, conductive, and flexible films made as a composite of bacterial cellulose microfibers (BMF), a polymer (either PVA or PEO), and silver nanowires (AgNWs) as viable and cost‐effective replacements to commercial indium‐tin oxide (ITO) and fluorine‐doped tin oxide (FTO) transparent conductors. The studies conducted indicate that the optical and mechanical properties of BMF‐polymer substrates are tuneable by varying the ratio of BMF to polymer. An optimized ratio of 70:30 of BMF to polymer was established for BMF‐PVA and BMF‐PEO composites. The optimized composite films were coated with varying amounts of AgNWs. As the AgNW loading increased, the deposition density of AgNW networks increased, while the sheet resistance and optical transmittance decreased. The optimum AgNW loading was determined at 0.20 mg for both composite films. The BMF‐PVA‐AgNW film displayed transmittance between 81% and 71% and an average resistivity of 9.462 ± 0.588 Ω/sq while the BMF‐PEO‐AgNW films showed transmittance between 73% and 65% and an average resistivity of 9.388 ± 0.1.375 Ω/sq. These properties compared well to that of commercial ITO and FTO glass substrates. The findings promote cellulose‐based composites as low‐cost, lightweight, and durable substrates for optoelectronic applications.
Combined treatment of high‐intensity interval training with neural stem cell generation on contusive model of spinal cord injury in rats
Authors: Keikhaei Reza , Abdi Elahe , Darvishi Marzieh , Ghotbeddin Zohreh , Hamidabadi Hatef Ghasemi 
Published: May 2023

AbstractIntroductionSpinal cord injury (SCI) leads to inflammation, axonal degeneration, and gliosis. A combined treatment of exercise and neural stem cells (NSC) has been proposed to improve neural repair. This study evaluated a combined treatment of high‐intensity interval training (HIIT) with NSC generation from adipose‐derived stem cells (ADSCs) on a contusive model of SCI in rats.Materials and MethodsIn vitro, rat ADSCs were isolated from the perinephric regions of Sprague–Dawley rats using enzymatic digestion. The ADSCs were transdifferentiated into neurospheres using B27, EGF, and bFGF. After production of NSC, they were labeled using green fluorescent protein (GFP). For the in vivo study, rats were divided into eight groups: control group, sham operation group, sham operation + HIIT group, sham operation + NSC group, SCI group, SCI + HIIT group, SCI + NSC group, and SCI/HIIT/NSC group. Laminectomy was carried out at the T12 level using the impactor system. HIIT was performed three times per week. To assess behavioral function, the Basso–Beattie–Bresnahan (BBB) locomotor test and H‐reflex was carried out once a week for 12 weeks. We examined glial fibrillary acidic protein (GFAP), S100β, and NF200 expression.ResultsNSC transplantation, HIIT and combined therapy with NSC transplantation, and the HIIT protocol improved locomotor function with decreased maximum H to maximum M reflexes (H/M ratio) and increased the Basso–Beattie–Bresnahan score.ConclusionCombined therapy in contused rats using the HIIT protocol and neurosphere‐derived NSC transplantation improves functional and histological outcomes.
Preclinical assessment of IRDye800CW‐labeled gastrin‐releasing peptide receptor‐targeting peptide for near infrared‐II imaging of brain malignancies
Authors: Zhang Yuan , Wang Li , Zhang Chengkai , Zhang Jingjing , Yuan Linhao , Jin Shucheng , Zhou Wenjianlong , Guan Xiudong , Kang Peng , Zhang Chuanbao , Tian Jie , Chen Xiaoyuan , Li Deling , Jia Wang 
Published: May 2023

AbstractWe aimed to develop a new biocompatible gastrin‐releasing peptide receptor (GRPR) targeted optical probe, IRDye800‐RM26, for fluorescence image‐guided surgery (FGS) of brain malignancies in near‐infrared window II (NIR‐II) imaging. We developed a novel GRPR targeting probe using a nine‐amino‐acid bombesin antagonist analog RM26 combined with IRDye800CW, and explored the fluorescent probe according to optical properties. Fluorescence imaging characterization in NIR‐I/II region was performed in vitro and in vivo. Following simulated NIR‐II image‐guided surgery, we obtained time‐fluorescent intensity curves and time‐signal and background ratio curves. Further, we used histological sections of brain from tumor‐beating mice model to compare imaging specificity between 5‐aminolevulinic acid (5‐ALA) and IRDye800‐RM26, and evaluated biodistribution and biocompatibility. IRDye800‐RM26 had broad emission ranging from 800 to 1200 nm, showing considerable fluorescent intensity in NIR‐II region. High‐resolution NIR‐II imaging of IRDye800‐RM26 can enhance the advantages of NIR‐I imaging. Dynamic and real time fluorescence imaging in NIR‐II region showed that the probe can be used to treat brain malignancies in mice between 12 and 24 h post injection. Its specificity in targeting glioblastoma was superior to 5‐ALA. Biodistribution analysis indicated IRDye800‐RM26 excretion in the kidney and liver. Histological and blood test analyses did not reveal acute severe toxicities in mice treated with effective dose (40 μg) of the probe for NIR‐II imaging. Because of the considerable fluorescent intensity in NIR‐II region and high spatial resolution, biocompatible and excretable IRDye800‐RM26 holds great potentials for FGS, and is essential for translation into human use.
Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in CHO cells via downregulation of PDGFRa and IRE1a signaling
Authors: Castellano Brian M. , Tang Danming , Marsters Scot , Lam Cynthia , Liu Peter , Rose Christopher M. , Sandoval Wendy , Ashkenazi Avi , Snedecor Brad , Misaghi Shahram 
Published: May 2023

AbstractDuring the course of biopharmaceutical production, heterologous protein expression in Chinese hamster ovary (CHO) cells imposes a high proteostatic burden that requires cellular adaptation. To mitigate such burden, cells utilize the unfolded protein response (UPR), which increases endoplasmic reticulum (ER) capacity to accommodate elevated rates of protein synthesis and folding. In this study, we show that during production the UPR regulates growth factor signaling to modulate growth and protein synthesis. Specifically, the protein kinase R‐like ER kinase (PERK) branch of the UPR is responsible for transcriptional down‐regulation of platelet‐derived growth factor receptor alpha (PDGFRa) and attenuation of the IRE1‐alpha (IRE1a) branch of the UPR. PERK knockout (KO) cell lines displayed reduced growth and viability due to higher rates of apoptosis despite having stabilized PDGFRa levels. Knocking out PERK in an apoptosis impaired (Bax/Bak double KO) antibody‐expressing cell line prevented apoptotic cell death and revealed that apoptosis was likely triggered by increased ER stress and reactive oxygen species levels in the PERK KO hosts. Our findings suggest that attenuation of IRE1a and PDGFRa signaling by the PERK branch of the UPR reduces ER protein folding capacity and hence specific productivity of CHO cells in order to mitigate UPR and prevent apoptotic cell death. Last, Bax/Bak/PERK triple KO CHO cell lines displayed 2–3 folds higher specific productivity and titer (up to 8 g/L), suggesting that modulation of PERK signaling during production processes can greatly improve specific productivity in CHO cells.
The impact of radiosensitivity on clinical outcomes of spinal metastases treated with stereotactic body radiotherapy
Authors: Guo Lanlan , Xu Qingqing , Ke Lixin , Wu Ziwei , Zeng Ziyi , Chen Lei , Chen Yuanyuan , Lu Lixia 
Published: May 2023

AbstractBackgroundTo evaluate the impact of radiosensitivity on outcomes of spinal metastases treated with stereotactic body radiotherapy (SBRT) and identify the correlated prognostic factors.MethodsThe authors retrospectively reviewed the records of all patients who underwent SBRT with no prior radiation for spinal metastases between October 2015 and October 2020 at Sun Yat‐sen University Cancer Center. On the basis of radiosensitivity, patients were divided into two groups—radiosensitive and radioresistant. The endpoints included local control (LC), overall survival (OS), pain relief, and time to pain relief.ResultsA total of 259 (82.5%) patients with 451 lesions were assessable with a median follow‐up time of 10.53 months. The 1‐, 2‐, and 3‐year OS rates were 59%, 52%, and 44%, respectively. The median survival was 33.17 months. Higher Karnofsky Performance Scale score and shorter time to diagnosis of spinal metastases from primary cancer at consult predicted for better OS (p = 0.02 and p < 0.001, respectively). The presence of other metastases (p = 0.04) and pain at enrollment assessed by the Brief Pain Inventory predicted for worse OS (p = 0.01). The 6‐, 12‐, and 24‐month LC rates were 88%, 86%, and 82%, respectively. Younger age was identified for better LC and pain relief (p < 0.001 and p = 0.04, respectively). There was no variable independently associated with time to pain relief. As for toxicity, no Grade ≥3 toxicity was observed.ConclusionsRegardless of radiosensitivity, SBRT is feasible and appears to be an effective treatment paradigm for patients with spinal metastases, with limited accepted toxicities.
Impact of the COVID‐19 pandemic on cancer healthcare utilization in southwestern China to March 2021
Authors: Li Peiyi , Zhu Yajuan , Wang Yaqiang , Liu Xiaoyu , Fang Xiang , Hou Yuanxin , Zheng Rujun , Li Junying , Zhang Bo , Chen Zhuo , Wang Chengdi , Zhu Tao , Li Weimin , Han Xuesong 
Published: May 2023

AbstractBackgroundOncological care has been disrupted worldwide during the COVID‐19 pandemic. We aimed to quantify the long‐term impact of the pandemic on cancer care utilization and to examine how this impact varied by sociodemographic and clinical factors in southwestern China, where the Dynamic Zero‐COVID Strategy was implemented. This strategy mainly included lockdowns, stringent testing, and travel restrictions to prevent the spread of COVID‐19.MethodWe identified 859,497 episodes of the utilization of cancer care from electronic medical records between January 1, 2019, and March 31, 2021, from the cancer center of a tertiary hospital serving an estimated population of 8.4 million in southwestern China. Changes in weekly utilization were evaluated via segmented Poisson regression across service categories, stratified by cancer type and sociodemographic factors.ResultsA sharp reduction in utilization of in‐person cancer services occurred during the first week of the pandemic outbreak in January 2020, followed by a quick rebound in February 2020. Although there were few COVID‐19 cases from March 2020 until this analysis, the recovery of most in‐person services was slow and remained incomplete as of March 31, 2021. The exceptions were outpatient radiation and surgery, which increased and exceeded pre‐pandemic levels, particularly among lung cancer patients; meanwhile, telemedicine utilization increased substantially after the onset of the pandemic. Care disruptions were most prominent for women, rural residents, uninsured, and breast cancer patients.ConclusionsAs of March 2021, despite few COVID‐19 cases, the COVID‐19 pandemic has had a strong and continuing impact on in‐person oncology care utilization in southwestern China under the Dynamic Zero‐COVID Strategy. Equitable and timely access to cancer care requires adjustment in strict policies for COVID‐19 prevention and control, as well as targeted remedies for the most vulnerable populations during and beyond the pandemic. Future studies should monitor the long‐term effects of the COVID‐19 pandemic and response strategies on cancer care and outcomes.
So curious that I want to buy it: The positive effect of queue wait on consumers' purchase intentions
Authors: Wang Tingting , Liang Shuyu , Sun Yixia 
Published: May 2023

AbstractQueue wait is a prevalent daily experience, with most prior research documenting its negative consequences on consumer outcomes. Recent research has suggested otherwise, revealing potential positive effects of queue wait. However, studies on the positive queue effects are rather limited and have largely neglected the role of consumers' affective experience in driving potential favorable consumer outcomes. Expanding this stream of research, in this article, we build on the queue wait literature and the curiosity literature to propose that the presence of queue waits generates feelings of curiosity and more importantly such curious feelings influence consumers' decision‐making in fashions that enhance their purchase intentions. The boundary condition for this effect was also examined. Five studies provided empirical evidence: the presence versus absence of queue wait enhances participants' purchase intentions that carry real consequences (Study 1); this effect is mediated by participants' feelings of curiosity after accounting for quality (Study 2) or after controlling for quality (Studies 1, 3–5), and is stronger among participants with hedonic versus utilitarian consumption motivation (Studies 4 and 5). By documenting the curiosity account for the positive effect of queue wait on consumers' purchase intentions, this research adds to extant research on positive queue effects and expands the curiosity literature into the queue wait context. The observed moderating effect of hedonic versus utilitarian consumption motivation echoes with the curiosity account. Practical implications for marketers were discussed.
Identifying E3 Ligase Substrates With Quantitative Degradation Proteomics
Authors: Jordan Victoria N. , Ordureau Alban , An Heeseon 
Published: May 2023

AbstractControlled protein degradation by the ubiquitin‐proteasome pathway is critical for almost all cellular processes. E3 ubiquitin ligases are responsible for targeting proteins for ubiquitylation and subsequent proteasomal degradation with spatial and temporal precision. While studies have revealed various E3‐substrate pairs involved in distinct biological processes, the complete substrate profiles of individual E3 ligases are largely unknown. Here we report a new approach to identify substrates of an E3 ligase for proteasomal degradation using unnatural amino acid incorporation pulse‐chase proteomics (degradomics). Applying this approach, we determine the steady‐state substrates of the C‐terminal to LisH (CTLH) E3 ligase, a multi‐component complex with poorly defined substrates. By comparing the proteome degradation profiles of active and inactive CTLH‐expressing cells, we successfully identify previously known and new potential substrates of CTLH ligase. Altogether, degradomics can comprehensively identify degradation substrates of an E3 ligase, which can be adapted for other E3 ligases in various cellular contexts.
Splenic hematoma following acute pancreatitis, and the role of conservative management: A case report
Authors: Ali Zulfeqar , Karim M. Masood , Javeed Maria , Khan Qaisar Ali , Farkou Christopher , Zepeda David , Abdi Parsa , Anthony Michelle , Farkouh Matthew , Khan Arooba , Ghosh Bikona 
Published: May 2023

Key clinical messageWe reported a unique case in which the patient developed splenic hematoma following acute pancreatitis, the condition responded well to conservative management without any surgical intervention.AbstractSplenic hematoma following acute pancreatitis is a rare complication that is thought to be due to the distribution of pancreatic exudates to the spleen. We presented a case of a 44‐year‐old patient with acute pancreatitis who developed a splenic hematoma. He responded well to conservative management and the hematoma was resolved.
No, it is not mutually exclusive! A case report of a girl with two genetic diagnoses: Craniofrontonasal dysplasia and pontocerebellar hypoplasia type 1B
Authors: Ibrahim Iman , Scriver Tara , Basalom Shuaa A. 
Published: May 2023

Key Clinical MessageMultiple genetic disorders can coexist in one patient. When the phenotype is not fully explained with one diagnosis, it is recommended to perform further genetic investigations in search for coexisting second diagnosis.AbstractCraniofrontonasal dysplasia (CFND) (MIM: 304110) is an X‐linked dominant disorder that shows paradoxically greater severity in heterozygous females than in hemizygous males. It is caused by a pathogenic variant in EFNB1. Pontocerebellar hypoplasia type 1B (PCH1B) (MIM: 614678) is an extremely rare condition with over 100 individuals reported to date. It is caused by biallelic pathogenic variants in EXOSC3. This report presents the case of a girl who was diagnosed prenatally with CFND based on the findings on the prenatal imaging and the known diagnosis of CFND in her mother. She has severe global development delay that cannot be explained solely by the CFND diagnosis. Around the age of 2 years, she was diagnosed with PCH1B following whole exome sequencing (WES) testing. The objective of this study is to highlight the importance of pursuing genetic investigation if the available genetic diagnosis cannot fully explain the clinical picture. This is a case report of one patient and review of the literature. Informed consent was obtained from the parents. WES was performed by a private lab using next‐generation sequencing (NGS), DNA was sequenced on the NovaSeq 6000 using 2 × 150 bp paired‐end read. WES identified the following: homozygous pathogenic variant in EXOSC3: C.395A>C, p.ASp132Ala, maternally inherited, likely pathogenic duplication at Xq13.1 (includes EFNB1) and paternally inherited 16p11.2 duplication that is classified as a variant of uncertain significance. Perusing more extensive genetic testing like: WES is indicated if the current genetic diagnosis cannot fully explain the phenotype in a patient.
Structural Disorder of AlMg‐Oxide Phase Supporting Cu/ZnO Catalyst Improves Efficiency and Selectivity for CO2 Hydrogenation to Methanol
Authors: Mavrič Andraž , Žerjav Gregor , Belec Blaž , Roškarič Matevž , Finšgar Matjaž , Pintar Albin , Valant Matjaž 
Published: May 2023

AbstractThe performance of the Cu/ZnO catalyst system with the AlMg‐oxide phase is studied for CO2 hydrogenation to methanol. The catalyst is prepared by thermal treatment of the hydrotalcite phase containing intimately mixed metal cations in the hydroxide form. CuO in the presence of ZnO and disordered AlMg‐oxide phase gets easily reduced to Cu during the hydrogenation reaction. Its catalytic activity at relatively low Cu metal content (∼14 at.%) remains stable during 100 hours on stream at 260 °C with constant space‐time yield for methanol (∼1.8 gMeOH gcat−1 h−1) and high methanol selectivity (>85 %) The improved performance is attributed to the neutralization of surface acidity, increased number of weak basic sites in the disordered phase, and lower tendency for coke formation.
Extended Light Absorption and Improved Charge Separation by Protonation of the Organic Ligand in a Bismuth‐Based Metal‐Organic Framework
Authors: Lei Longfei , Liu Yuanyuan , Zhang Yujia , Zhang Caiyun , Li Yujie , Wang Zeyan , Wang Peng , Zheng Zhaoke , Cheng Hefeng , Dai Ying , Huang Baibiao 
Published: May 2023

AbstractIn this work, a new method to extend the light absorption and improve the photocatalytic activity of metal‐organic frameworks (MOFs) with nitrogen‐containing ligand is reported, namely, the protonation of nitrogen. Specifically, a protonated Bi‐based MOF synthesized by a hydrothermal method (Bi‐MMTAA‐H, MMTAA=2‐mercapto‐4‐methyl‐5‐thiazoleacetic acid) displays a wider visible light absorption than Bi‐MMTAA‐R with the same single‐crystal structure, but synthesized by a reflux method. The redshifted light absorption was confirmed to be caused by the protonation of nitrogen in the thiazolyl ring in MMTAA. Moreover, this protonation also facilitates the charge separation and transfer and improves the photocatalytic activity of selective oxidation of α‐terpinene to p‐cymene. Our results provide a new idea for nitrogen‐containing Bi‐based MOFs to extend the light absorption and improve the photocatalytic performance.
Boosting Methanol‐Mediated CO2Hydrogenation into Aromatics by Synergistically Tailoring Oxygen Vacancy and Acid Site Properties of Multifunctional Catalyst
Authors: Wang Wenhang , He Ruosong , Wang Yang , Li Meng , Liu Jianxin , Liang Jiaming , Yasuda Shuhei , Liu Qiang , Wu Mingbo , Tsubaki Noritatsu 
Published: May 2023

AbstractEven though the direct hydrogenation of CO2into aromatics has been realized via a methanol‐mediated pathway and multifunctional catalyst, few works have been focused on the simultaneously rational design of each component in multifunctional catalyst to improve the performance. Also, the structure‐function relationship between aromatics synthesis performance and the different catalytic components (reducible metal oxide and acidic zeolite) has been rarely investigated. Herein, we increase the oxygen vacancy (Ov) density in reducible Cr2O3by sequential carbonization and oxidation (SCO) treatments of Cr‐based metal–organic frameworks. Thanks to the enriched Ov, Cr2O3‐based catalyst affords high methanol selectivity of 98.1 % (without CO) at a CO2conversion of 16.8 % under high reaction temperature (350 °C). Furthermore, after combining with the acidic zeolite H‐ZSM‐5, the multifunctional catalyst realizes the direct conversion of CO2into aromatics with conversion and selectivity as high as 25.4 % and 80.1 % (without CO), respectively. The property of acid site in H‐ZSM‐5, especially the Al species that located at the intersection of straight and sinusoidal channels, plays a vital role in enhancing the aromatics selectivity, which can be precisely controlled by varying the hydrothermal synthesis conditions. Our work provides a synergistic strategy to boost the aromatics synthesis performance from CO2hydrogenation.