bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bina Usaha Kesehatan Sekolah “Layanan Monitoring Visual Chilhood Developmnet Berbasis Online”
Authors: Dewi Satiti Ika Arum , Wahyunirum Ari Damayanti 
Published: September 2023

Pembelajaran blended learning merupakan metode pembelajaran yang diadaptasi pada era new normal. Pembelajaran menganut sistem daring dan luring. Meotde daring mengharuskan anak belajar melalui media elektronik secara kontinu. Kegiatan ini memiliki dampak pada kesehatan fisik anak terutama adalah kesehatan penglihatan. Kelainan penglihatan pada anak dapat menyebabkan beberapa masalah dianataranya adalah penurunan prestasi belajar, penurunan kemampuan sosialisasi dan koordinasi gerak motoric yang menurun sehingga tumbuh kembang anak tidak dapat meningkat secara optimal. Usaha Kesehatan Sekolah (UKS) merupakan sarana kesehatan awal yang dapat diakses oleh para siswa di sekolah. Upaya kesehatan pencegahan gangguan mata perlu digalakkan oleh UKS demi mendukung proses pembelajaran. Kegiatan pengabdian ini bertujuan untuk menggunakan aplikasi MOVIE (Monitoring Visual Chilhood Development) sebagai skrining gangguan mata dengan mengoptimalkan peran UKS. Kegiatan pengabdian ini menghasilkan capaian peningkatan pengetahuan dan keterampilan siswa dengan mayoritas baik sebesar 41% dan 50%. Kegiatan skrining ketajaman mata mendapatkan data mayoritas berada ketajaman 70%-89% sebanyak 44%. Kemudian pelatihan dan pendampingan aplikasi MOVIE juga meninggatkan keterampilan penggunaan aplikasi MOVIE pada guru 100% dalam katergori baik. Kesimpulan dari kegiatan pengabdian kepada masyarakat ini adalah adanya peningkatan pengetahuan dan keterampilan siswa serta guru terhadap penggunaan aplikasi MOVIE. Kegiatan pengabdian masyarakat ini dinilai sangat bermanfaat dan penting, karena diharapkan dapat menunjang kemajuan teknologi pembelajaran daring dan meningkatkan prestasi belajar siswa.
The Perception of Secondary School Teachers on the Availability of Counseling Services for Students With Learning Disabilities
Authors: Alghazo Emad M. , Sharadgah Maher Tayseer 
Published: September 2023

The current research aimed to assess the status of counseling services offered to students with Learning Disabilities (SLDs) in Macca as perceived by their teachers. The research methodology employed was descriptive. The sample consisted of 330 teachers (174 males and 156 females) who work in the government schools in Macca during the 2021 academic year. The data was collected using a questionnaire that covered three areas: 1) Psychological counseling services (PCS) consisting of 12 items, 2) Social counseling services (SCS) consisting of 10 items, and 3) Educational counseling services (ECS) consisting of 9 items. The questionnaire was validated and its reliability was established.
The findings revealed that the counseling requirements of secondary school students with Learning Disabilities (SLDs) in Macca, as perceived by their teachers, had a moderate average mean of 2.83. Additionally, the educational counseling services dimension was the top priority with an average mean of 2.88 and a moderate level of consistency, followed by psychological counseling services with an average mean of 2.81 and a moderate level of consistency. Lastly, the social counseling services dimension had the lowest average mean of 2.80 and a moderate level of consistency.
The results also indicated that there were no statistically significant differences in the responses about the availability of counseling services for secondary school students with Specific Learning Disabilities (SLDs) in Macca based on gender, academic background, work environment, years of experience, or the overall survey instrument. The study suggested establishing clear guidelines for counselors to effectively deliver the necessary counseling services for SLDs.
Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma
Authors: Cao Feng , Qi Yong , Wu Wenyong , Li Xutong , Yang Chuang 
Published: September 2023

Background: Cuproptosis, as a recently discovered type of programmed cell death, occupies a very important role in hepatocellular carcinoma (HCC) and provides new methods for immunotherapy; however, the functions of cuproptosis in HCC are still unclear.Methods: We first analyzed the transcriptome data and clinical information of 526 HCC patients using multiple algorithms in R language and extensively described the copy number variation, prognostic and immune infiltration characteristics of cuproptosis related genes (CRGs). Then, the hub CRG related genes associated with prognosis through LASSO and Cox regression analyses and constructed a prognostic prediction model including multiple molecular markers and clinicopathological parameters through training cohorts, then this model was verified by test cohorts. On the basis of the model, the clinicopathological indicators, immune infiltration and tumor microenvironment characteristics of HCC patients were further explored via bioinformation analysis. Then, We further explored the key gene biological function by single-cell analysis, cell viability and transwell experiments. Meantime, we also explored the molecular docking of the hub genes.Results: We have screened 5 hub genes associated with HCC prognosis and constructed a prognosis prediction scoring model. And the model results showed that patients in the high-risk group had poor prognosis and the expression levels of multiple immune markers, including PD-L1, CD276 and CTLA4, were higher than those patients in the low-risk group. We found a significant correlation between risk score and M0 macrophages and memory CD4+ T cells. And the single-cell analysis and molecular experiments showed that BEX1 were higher expressed in HCC tissues and deletion inhibited the proliferation, invasion and migration and EMT pathway of HCC cells. Finally, it was observed that BEX1 could bind to sorafenib to form a stable conformation.Conclusion: The study not only revealed the multiomics characteristics of CRGs in HCC but also constructed a new high-accuracy prognostic prediction model. Meanwhile, BEX1 were also identified as hub genes that can mediate the cuproptosis of hepatocytes as potential therapeutic targets for HCC.
Shotgun-metagenomics reveals a highly diverse and communal microbial network present in the drains of three beef-processing plants
Authors: Palanisamy Vignesh , Bosilevac Joseph M. , Barkhouse Darryll A. , Velez Sarah E. , Chitlapilly Dass Sapna 
Published: September 2023

BackgroundMulti-species biofilms pose a problem in various environments, especially food-processing environments. The diversity of microorganisms in these biofilms plays a critical role in their integrity and protection against external biotic and abiotic factors. Compared to single-species biofilms, mixed-species biofilms are more resistant to various stresses, including antimicrobials like sanitizers. Therefore, understanding the microbiome composition and diversity in biofilms and their metabolic potential is a priority when developing intervention techniques to combat foodborne pathogens in food processing environments. MethodsThis study aimed to describe and compare the microbiome profile of 75 drain biofilm samples obtained from five different locations (Hotscale, Hotbox, Cooler, Processing, &amp; Grind room) of three beef-processing plants (Plant A, B &amp; C) taken over two timepoints 2017-18 (T1) and 2021 (T2) by shotgun sequencingResultsCore microbiome analysis found Pseudomonas, Psychrobacter, and Acinetobacter to be the top three prevalent genera among the plants and locations. Alpha diversity analysis demonstrated a high diversity of microbiome present in all the plants and locations across the time points. Functional analysis showed the high metabolic potential of the microbial community with abundance of genes in metabolism, cell-adhesion, motility, and quorum sensing. Moreover, Quaternary Ammonium Compound (QAC) resistance genes were also observed, this is significant as QAC sanitizers are commonly used in many food processing facilities. Multi-functional genes such as transposases, polymerases, permeases, flagellar proteins, and Mobile Genetic Elements (MGEs) were found suggesting these are dynamic microbial communities that work together to protect themselves against environmental stresses through multiple defense mechanisms.ConclusionThis study provides a framework for understanding the collective microbial network spanning a beef processing system. The results can be used to develop intervention strategies to best control these highly communicative microbial networks.
Characteristics of carbohydrate counting practice associated with adequacy of glycated hemoglobin in adults with type 1 diabetes mellitus in Brazil
Authors: Uliana Gabriela Correia , Camara Lediane Nunes , Paracampo Carla Cristina Paiva , da Costa Juliana Carvalho , Gomes Daniela Lopes 
Published: September 2023

BackgroundThe Carbohydrate Counting (CC) is directly associated with achieving glycemic control by people with Type 1 Diabetes Mellitus (T1DM). Therefore, this study aims to analyze characteristics of the CC practice associated with the adequacy of glycated hemoglobin (HbA1c) in adults with T1DM in Brazil.MethodsThe study was cross-sectional, carried out using an online form with questions about knowledge of CC, clinical, anthropometric, sociodemographic data, follow-up with health professionals and understanding of the concepts of CC. Pearson’s chi-square test and binomial logistic regression analysis (p&lt;0.05) were applied.Results173 adults participated, of which 57.2% had increased HbA1c (≥7%). Having the diabetes duration &lt;10 years (p=0.006), performing the CC at lunch (p=0.040) and dinner (p=0.018), using specific applications to perform the CC (p=0.001), having learned to perform CC with a nutritionist (p=0.037) and knowing how to correctly define the concepts of food bolus (p=0.001), correction bolus (p&lt;0.001) and insulin/carbohydrate ratio (p&lt;0.001) was associated with having adequate HbA1c (&lt;7%). Participants who were undergoing CC practice were 3.273 times more likely to have adequate HbA1c and participants with diabetes duration &lt;10 years were 2.686 times more likely to have adequate HbA1c.ConclusionIt was concluded that variables transversal to CC favor adequate HbA1c values in adults with T1DM and that practicing CC and having a diabetes duration of less than 10 years are predictive factors of having adequate HbA1c.
Prevalence of osteoporosis in patients awaiting unicompartmental knee arthroplasty: a cross-sectional study
Authors: Zhuang Zhikun , Huang Changyu , Chen Xianyi , Wei Qiushi , Guo Jinhua , Xu Zhiqing , Wu Rongkai , Wu Zhaoke , Xu Fudong 
Published: September 2023

ObjectivesOsteoporosis may contribute to failure of unicompartmental knee arthroplasty (UKA), yet the prevalence of osteoporosis in the population awaiting UKA has not been adequately studied. The objectives of this study were to report the prevalence of osteoporosis in people awaiting UKA and the rate of anti-osteoporosis treatment, and to explore factors associated with osteoporosis prevalence in people awaiting UKA.MethodsParticipants awaiting UKA from January 2019 to May 2023 were consecutively enrolled. Participants ‘ age, gender, BMI, knee K-L score, VAS score, history of previous DXA testing, history of anti-osteoporosis treatment, and possible underlying risk factors were recorded. All participants were given a dual-energy x-ray absorptiometry (DXA) test after the visit. The diagnosis of osteoporosis was made according to the World Health Organization criteria. Compare the prevalence of osteoporosis between people waiting for UKA and the general population. Risk factors associated with osteoporosis were analyzed using multiple linear regression and binary logistic regression models.ResultsA total of 340 participants were included in the study, 259 in female and 81 in male, with a mean age of 63.53 years (range: 41-84 years), and all participants completed UKA and had DXA prior to UKA. The prevalence of osteoporosis was 40.88% (44.79% in female and 28.40% in male). The prevalence of osteoporosis was higher in female than in male (p&lt;0.001). The prevalence of osteoporosis in the population waiting for UKA was significantly higher than that in the general population (p &lt; 0.001). DXA testing was performed in 12.06% within 1 year prior to the visit. The percentage of those who had received anti-osteoporosis treatment was 20.59% (20.86% in osteoporosis, 22.39% in Osteopenia and 16.42% in normal bone mass). The correlation between age, gender, body mass index, visual analogue scale score and osteoporosis was statistically significant.ConclusionOsteoporosis is common in people waiting for UKA, but screening and treatment rates are low. Female patients of advanced age and low weight combined with significant pain should be considered for osteoporosis screening and appropriate treatment before UKA.
Stability mechanisms of soft rock mining roadways through roof cutting and pressure relief: an exploratory model experiment
Authors: Zhu Qingwen , Li Tingchun , Lou Qingnan , Liu Yishuai , Li Chunping , Chen Jiangang 
Published: September 2023

IntroductionSoft rock mining roadways are severely deformed and damaged during coal mining. Blindly increasing the support strength not only has little effect but also wastes material resources.MethodsMaintaining the original support parameters, model experiments were conducted to investigate the mechanism of pressure relief protection of the front soft rock mining roadway by cutting the roof behind the longwall face. The roof-cutting height was 2.5 times the coal thickness, the angle was 10°, and the advance distance is 0. ResultsThe study found that the abutment stress borne by the roof of the original roadway was transferred to the coal seams to be mined. The average stress of the coal seams increased by 10%, while the average stress of the surrounding rock in the front roadway decreased by 12.57%. The roof cutting weakened the influence of the overlying strata in the gob on the rear roadway. The stability of the rear roadway also weakened the traction effect on the front roadway. The vertical convergence of the front roadway decreased by 27.3%, and the deformation of the coal pillars decreased by 15.7%.DiscussionThe roof cutting reduced the stress of the front roadway to the peak failure stress, fundamentally weakening the main factor that induced the deformation of the front roadway. Numerical simulations were performed to research the deformation and stress distribution properties of the surrounding rock after roof cutting, and the model experimental results were validated. Finally, engineering recommendations are presented, which are expected to provide a reference for controlling the roadway stability of soft rock masses.
The immune checkpoint adenosine 2A receptor is associated with aggressive clinical outcomes and reflects an immunosuppressive tumor microenvironment in human breast cancer
Authors: Zohair Basma , Chraa Dounia , Rezouki Ibtissam , Benthami Hamza , Razzouki Ibtissam , Elkarroumi Mohamed , Olive Daniel , Karkouri Mehdi , Badou Abdallah 
Published: September 2023

BackgroundThe crosstalk between the immune system and cancer cells has aroused considerable interest over the past decades. To escape immune surveillance cancer cells evolve various strategies orchestrating tumor microenvironment. The discovery of the inhibitory immune checkpoints was a major breakthrough due to their crucial contribution to immune evasion. The A2AR receptor represents one of the most essential pathways within the TME. It is involved in several processes such as hypoxia, tumor progression, and chemoresistance. However, its clinical and immunological significance in human breast cancer remains elusive.MethodsThe mRNA expression and protein analysis were performed by RT-qPCR and immunohistochemistry. The log-rank (Mantel-Cox) test was used to estimate Kaplan-Meier analysis for overall survival. Using large-scale microarray data (METABRIC), digital cytometry was conducted to estimate cell abundance. Analysis was performed using RStudio software (7.8 + 2023.03.0) with EPIC, CIBERSORT, and ImmuneCellAI algorithms. Tumor purity, stromal and immune scores were calculated using the ESTIMATE computational method. Finally, analysis of gene set enrichment (GSEA) and the TISCH2 scRNA-seq database were carried out.ResultsGene and protein analysis showed that A2AR was overexpressed in breast tumors and was significantly associated with high grade, elevated Ki-67, aggressive molecular and histological subtypes, as well as poor survival. On tumor infiltrating immune cells, A2AR was found to correlate positively with PD-1 and negatively with CTLA-4. On the other hand, our findings disclosed more profuse infiltration of protumoral cells such as M0 and M2 macrophages, Tregs, endothelial and exhausted CD8+ T cells within A2ARhigh tumors. According to the Single-Cell database, A2AR is expressed in malignant, stromal and immune cells. Moreover, it is related to tumor purity, stromal and immune scores. Our results also revealed that CD8+T cells from A2ARhigh patients exhibited an exhausted functional profile. Finally, GSEA analysis highlighted the association of A2AR with biological mechanisms involved in tumor escape and progression.ConclusionThe present study is the first to elucidate the clinical and immunological relevance of A2AR in breast cancer patients. In light of these findings, A2AR could be deemed a promising therapeutic target to overcome immune evasion prevailing within the TME of breast cancer patients.
Influence of genetically predicted autoimmune diseases on NAFLD
Authors: Xu Min , Wu Tong , Li Zhaoxia , Xin Guijie 
Published: September 2023

IntroductionNon-alcoholic fatty liver disease (NAFLD), the emerging cause of end-stage liver disease, is the most common liver disease. Determining the independent risk factors of NAFLD and patients who need more monitoring is important.MethodsTwo-Sample Mendelian randomization (MR) was performed in the analysis to investigate the causal association of different autoimmune diseases with NAFLD using summary level data. Genome-wide association study (GWAS) of 5 autoimmune diseases including celiac disease (CeD), Crohn’s disease (CD), multiple sclerosis (MS), rheumatoid arthritis (RA), and type 1 diabetes (T1D) were selected for Instrument variables (IVs). NAFLD was included as outcome.ResultAfter adjusting for confounding factors, genetic predisposition of CeD (OR= 0.973, [0.949,0.997], IVW p-value=0.026), MS (OR= 1.048, [1.012,1.085], IVW p-value= 0.008), RA (OR= 1.036, [1.006,1.066], IVW p-value=0.019), T1D (OR= 1.039, [1.002,1.079], IVW p-value= 0.041) is causally associated with NAFLD. No causal effect was found between CD and NAFLD.ConclusionCeD itself may be a protective factor for NAFLD, the results of previous observational studies have been influenced by confounding factors, and the morbidity of NAFLD may be higher in patients with MS, RA, and T1D than in common populations, and monitoring the prevalence of NAFLD in these populations is considerable.
Serum pro-inflammatory biomarkers associated with improvement in quality of life in pulmonary tuberculosis
Authors: Carreto-Binaghi Laura E. , Sartillo-Mendoza Luis Gustavo , Muñoz-Torrico Marcela , Guzmán-Beltrán Silvia , Carranza Claudia , Torres Martha , González Yolanda , Juárez Esmeralda 
Published: September 2023

IntroductionPulmonary dysfunction is an underestimated complication in tuberculosis (TB) infection, affecting quality of life (QoL). Although respiratory function tests objectively reflect lung disturbances in a specific moment, predictors of illness severity at the time of diagnosis are still lacking.MethodsWe measured serum pro-inflammatory cytokines (TNF-α and IL-8), eicosanoids (PGE2, LTB4, RvD1, Mar1, and LXA4), a marker of tissue damage (cell-free nucleosomes), and indicators of redox status (malonaldehyde, 8-isoprostane, total oxidants, and antioxidants), as well as a score of radiological abnormalities (SRA) and a QoL questionnaire, in 25 patients with pulmonary TB at the time of diagnosis (t0) and two months after the initiation of treatment (t2).ResultsWe found higher antioxidant levels in the patients with the worst QoL at t0, and all the indicators of the prooxidant state were significantly reduced at t2, while the total antioxidant levels increased. LTB4, a pro-inflammatory eicosanoid, was diminished at t2, while all the pro-resolutory lipids decreased substantially. Significant correlations between the SRA and the QoL scores were observed, the latter showing a substantial reduction at t2, ranking it as a reliable tool for monitoring disease evolution during TB treatment.DiscussionThese results suggest that evaluating a combination of these markers might be a valuable predictor of QoL improvement and a treatment response indicator; in particular, the oxidation metabolites and eicosanoid ratios could also be proposed as a future target for adjuvant therapies to reduce inflammation-associated lung injury in TB disease.
Multi-omics analysis reveals the association between elevated KIF18B expression and unfavorable prognosis, immune evasion, and regulatory T cell activation in nasopharyngeal carcinoma
Authors: Tang Siqi , Wu Zhenyu , Chen Lusi , She Longjiang , Zuo Weihan , Luo Weijun , Zhang Yang , Liang Shaoqiang , Liu Guichao , He Biyi , He Jinfeng , Zhang Ning 
Published: September 2023

BackgroundNasopharyngeal carcinoma (NPC) is prevalent in Southern China. The expression profile and functions of kinesin family member 18B (KIF18B) remain unclear in NPC.MethodsBulk and single-cell transcriptome data for NPC were downloaded. KIF18B expression differences in NPC and normal tissues and its prognostic value were validated by immunohistochemistry and Cox model. We performed multi-faceted functional enrichment analysis on KIF18B. Immune infiltration was analyzed comprehensively by the CIBERSORT, EPIC, and quanTIseq algorithms and the BisqueRNA package and confirmed by immunofluorescence assay. The intercellular communication were investigated by the CellChat package. We explored the dynamics of KIF18B expression by pseudotime trajectory. M6A modification analysis rely on SRAMP platform. The treatment response were evaluated by Tumor Immune Dysfunction and Exclusion (TIDE) score, immunophenoscore and IC50 value.ResultsKIF18B overexpression in NPC led to unfavorable prognosis, and significantly associated with advanced T, N, and stage classifications. Functional analysis demonstrated that KIF18B was involved in immune suppression, epithelial-mesenchymal transition (EMT), N6-methyladenosine (m6A) modification and therapeutic responses. The deconvolution algorithm indicated that activated regulatory T cells (Tregs) had the strongest positive correlation with KIF18B among immune cells (R = 0.631). Validated by immunofluorescence assay, the high KIF18B expression group displayed a notable rise in Tregs infiltration, accompanied by a substantial decrease in the infiltration of CD8+ T cells and macrophages. In the intercellular communication network, malignant cells with high KIF18B expression implicated in more interactions, and activated and recruited Tregs by modulating cytokines, chemokines, and immune checkpoints. KIF18B was upregulated in more advanced malignant cells and influenced EMT by regulating ITGA6, VIM, and ZEB1/2. KIF18B expression was positively related to m6A “writer” and “reader” genes, and negatively related to “eraser” genes. The KIF18B high expression group exhibited a higher TIDE score and elevated IC50 values for the commonly used chemotherapy drugs, gemcitabine, oxaliplatin, and 5-fluorouracil.ConclusionKIF18B is a significant prognostic marker in NPC, and may modulate immune evasion and EMT. M6A modification may account for the aberrant overexpression of KIF18B in NPC. Furthermore, KIF18B may predict response to immunotherapy and chemotherapy.