bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coping Styles Mediated the Association Between Perceived Organizational Support and Resilience in Emergency Nurses Exposed to Workplace Violence: A Cross‐Sectional Study
Authors: Liao Liwen , Wu Qing , Su Ya , Li Rui , Wang Lin 
Published: January 2025

ABSTRACTResilience was a core factor for mitigating the risks of adverse impacts of workplace violence (WPV) and may be determined by perceived organizational support (POS) and coping styles. The aim of this study was to examine the potential mediation effects of coping styles between POS and resilience in emergency nurses exposed to WPV. Participants were 670 emergency nurses (84.0% female; mean age 30.94 [SD 6.13]) reporting WPV exposure in the past 3 months. Potential mediation effects of coping styles were explored using structural equation modeling. Results showed that POS, negative coping style, and positive coping style were associated with resilience in emergency nurses exposed to WPV. Coping styles mediated the association of POS with resilience. Two indirect pathways explained 21.8% and 22.4% of the variance in nurse resilience, respectively. The findings underscore the importance of POS and coping skills in promoting resilience of emergency nurses exposed to WPV. Nurse management programs are warranted to improve the resilience of emergency nurses at the organizational and individual levels.
Centromere diversity and its evolutionary impacts on plant karyotypes and plant reproduction
Authors: Steckenborn Stefan , Marques André 
Published: January 2025

SummaryKaryotype changes are a formidable evolutionary force by directly impacting cross‐incompatibility, gene dosage, genetic linkage, chromosome segregation, and meiotic recombination landscape. These changes often arise spontaneously and are commonly detected within plant lineages, even between closely related accessions. One element that can influence drastic karyotype changes after only one (or few) plant generations is the alteration of the centromere position, number, distribution, or even its strength. Here, we briefly explore how these different centromere configurations can directly result in karyotype rearrangements, impacting plant reproduction and meiotic recombination.
Biomechanical Analysis and Solution Suggestions of Screw Replacement Scenarios in Femoral Neck Fracture Surgeries: Finite Element Method
Authors: Güvercin Yılmaz , Yaylacı Murat , Dizdar Ayberk , Özdemir Mehmet Emin , Ay Sevil , Yaylacı Ecren Uzun , Karahasanoğlu Umitcan , Uygun Hüseyin , Peker Gökhan 
Published: January 2025

ABSTRACTObjectiveDespite several surgical options, there has yet to be a consensus on the best treatment for femoral neck fracture (FNF) due to higher complication rates compared to other bone fractures. This study aims to examine the possible consequences and solution suggestions of changing screws during surgery for various reasons in FNF surgical treatment from a biomechanical perspective.MethodFNF and treatment materials were analyzed biomechanically using a package program based on the finite element method (FEM). This study created a solid model with images of femur bone tomography. Dynamic hip screws (DHS), cannulated screws (CCS), and medial buttress plates (MBP) were obtained by making three‐dimensional designs. The required elements for the models were assigned, and the material properties of the elements were defined. The solutions were obtained as crack distance and deformation results after defining the boundary conditions and applying the necessary loading.ResultsThe strain and crack distance values created by six models on the fracture line under different parameters were obtained, and the numerical results were evaluated. The DHS and CCS models produced the highest crack distance and deformation values when all screws were loose. The lowest values were obtained in the intact‐85 model when all CCS were tight. When the results are evaluated, it is seen that the MBP has a decreasing effect on the results. Mechanical evaluation of six different options used in femoral neck fractures was performed. 85 mm CCS applied to our standard model gave the best results, while the use of 80 mm CCS in the same model showed promising results compared to other models. It is understood that CCS have the best stability even in loosening models with the medial support plate. Different models are from intact‐85 mm DHS+1CS+MBP to DHS+1CS, which was worked with LSR+USR‐2, according to decreasing stability.ConclusionThis study offers various biomechanical solutions to possible intraoperative problems in FNF treatment. The following results were obtained from the study data. When the CCS needs to be lengthened or replaced, it is appropriate to use the CCS with the MBP. A single anti‐rotation screw is sufficient for lag screw extensions of the DHS plate, and the MBP may be a savior procedure in surgery.
The regulatory module MdCPCL‐MdILR3L mediates the synthesis of ascorbic acid and anthocyanin in apple
Authors: Zou Qi , Bao Tiantian , Yu Lei , Xu Haifeng , Liu Wenjun , Li Zhiqiang , Zhu Yansong , Chen Ran , Hou Xukai , Zhang Zongying , Wang Nan , Chen Xuesen 
Published: January 2025

SummaryApple (Malus domestica Borkh.) is one of the most economically valuable fruit crops globally and a key dietary source for various nutrients. However, the levels of ascorbic acid (AsA) and anthocyanin, essential micronutrients for human health, are extremely low in the pulp of commonly cultivated apple varieties. In the present study, the second‐generation hybrid strain of Xinjiang red‐fleshed apple (‘Zihong No. 1’ × ‘Gala’) was used as the test material. The results revealed that AsA content was significantly higher in red‐fleshed apple pulp than in non‐red‐fleshed varieties, and the expression of MdGLDH, a key gene in the D‐mannose/L‐galactose pathway, correlated strongly with AsA levels. Using the promoter of MdGLDH as bait, an R3‐type MYB transcription factor (TF), MdCPC‐like, was identified through yeast one‐hybrid screening. Further analysis revealed that the overexpression of MdCPCL increased the AsA and anthocyanin levels in both callus and fruits, whereas MdCPCL knockdown led to a reduction in their levels. Moreover, the interaction between MdCPCL and the bHLH TF MdILR3‐like was confirmed, forming the MdCPCL‐MdILR3L complex. This complex significantly enhanced the transcription of downstream target genes MdGLDH and MdANS, promoting the synthesis of AsA and anthocyanins. This study contributes to further enrich the anabolic pathways of AsA and anthocyanin in apples and provides a theoretical foundation for the quality breeding of red‐fleshed apple varieties.
No national research assessment here. A Canadian counterfactual?
Authors: Barnes Trevor J. 
Published: January 2025

AbstractCanada has never established a national research assessment exercise for its post‐secondary institutions. This is a consequence of Canada's nineteenth century legal foundation as an independent nation‐state that made higher education a provincial rather than a federal responsibility. In this sense, the experience of Canada over the past four decades makes a counterfactual to the many countries that implemented national research assessments during the same period. Nonetheless, there have been sharp criticisms of the distribution of federal government research monies among researchers in Canadian universities and colleges. These criticisms are discussed along with the effects on Canadian higher education of neoliberalism. Neoliberalism is often identified as a cause of national research assessments. However, Canada is the seeming paradox of having its post‐secondary institutions subject to neoliberalism but not subject to national research assessments.
Patient and wound factors associated with WOUND‐Q scales measuring health‐related quality of life: An international cross‐sectional study
Authors: Simonsen Nina Vestergaard , Möller Sören , Rae Charlene , Klassen Anne F. , Poulsen Lotte , Pusic Andrea Louise , Sørensen Jens Ahm 
Published: January 2025

AbstractThe WOUND‐Q is a patient‐reported outcome measure for individuals with any type of chronic wound. This study aimed to identify patient and wound factors associated with the four WOUND‐Q health‐related quality of life (HRQL) scales: Life impact, Psychological, Sleep, and Social. Adults with a chronic wound were recruited internationally through clinical settings between August 2018 and May 2020, and through an online platform (i.e. Prolific) in September 2022. Multivariable linear regression analyses were conducted to identify factors significantly associated with the WOUND‐Q scales. The assessments obtained were 1273, 1275, 706, and 1256 for the Life Impact, Psychological, Sleep, and Social scales, respectively. The mean age of participants was 55 (SD = 18) years; most (66%) had a single wound, and most (56%) wounds had lasted more than 6 months. The most common causes were trauma, surgery, and diabetic foot ulcer. Wound characteristics associated with worse scores on at least one of the scales were drainage, vacuum treatment, aetiologies (i.e. diabetic foot ulcer, trauma, other, multiple), duration (i.e. 10–11 months), having four or more wounds, smell, and sleep interference, while wound location different from the face or neck was associated with better scores (p < 0.05). Patient factors associated with worse scores included having diabetes or a comorbidity, whereas increasing age or male gender were associated with better scores (p < 0.05). Sleep disturbances had the largest negative influence on HRQL scores. This study identified factors affecting HRQL in individuals with chronic wounds. Understanding these associations can inform better management and treatment strategies to improve HRQL for these patients.
Homology of spectral minimal partitions
Authors: Berkolaiko Gregory , Canzani Yaiza , Cox Graham , Marzuola Jeremy L. 
Published: January 2025

AbstractA spectral minimal partition of a manifold is its decomposition into disjoint open sets that minimizes a spectral energy functional. It is known that bipartite spectral minimal partitions coincide with nodal partitions of Courant‐sharp Laplacian eigenfunctions. However, almost all minimal partitions are non‐bipartite. To study those, we define a modified Laplacian operator and prove that the nodal partitions of its Courant‐sharp eigenfunctions are minimal within a certain topological class of partitions. This yields new results in the non‐bipartite case and recovers the above known result in the bipartite case. Our approach is based on tools from algebraic topology, which we illustrate by a number of examples where the topological types of partitions are characterized by relative homology.
Early introduction of oral feeding and its impact on full oral feeding and discharge in preterm infants: a systematic review protocol
Authors: Fujitsuka Maki , Takao Kenji , Ueki Shingo , Morokuma Seiichi 
Published: January 2025

Objective:
This review aims to examine the impact of early introduction of oral feeding, compared with standard care, on the duration of achieving full oral feeding, postmenstrual ages at full oral feeding and discharge, and weight gain in preterm infants.

Introduction:
Because of their immature oral function, preterm infants are prone to feeding difficulties during hospitalization and after hospital discharge. Early introduction of oral feeding helps infants to coordinate sucking, swallowing, and respiration, thereby improving their oral feeding skills. However, early introduction of oral feeding may also contribute to respiratory adverse events; therefore, safety verification is necessary.

Inclusion criteria:
Early introduction of oral feeding is defined as beginning oral feeding at < 33 weeks’ postmenstrual age and transitioning from tube feeding to oral feeding. The primary outcomes are the duration from the initial oral feeding to full oral feeding; postmenstrual age at full oral feeding and discharge; and weight gain. The secondary outcomes are oxygen desaturation and bradycardia.

Methods:
Eight databases, including MEDLINE (Ovid), CINAHL (EBSCOhost), and the Cochrane Central Register of Controlled Trials, will be used with no restrictions on language or publication year. Study selection, critical appraisal, data extraction, and data synthesis will be conducted by 2 independent reviewers according to the JBI methodology for systematic reviews. Quantitative data will be extracted and pooled in a statistical meta-analysis, if possible. The Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach will be used to assess the certainty of evidence.

Review registration:
PROSPERO CRD42024530910
Establishing a new human pneumococcal standard reference serum, MPRSS-01
Authors: Antonello Joseph M. , Murphy Rocio D. , Abeygunawardana Chitrananda , Malinverni Juliana C. , Gowrisankar Rajam , Green Tina , Greway Rebecca , Schmauch Jenna , Howlett Adrienne , Bonhomme Cyrille J. , Nolan Katrina M. 
Published: January 2025

ABSTRACT

Measuring the immunogenicity of pneumococcal vaccines involves the use of immunoassays to measure serotype-specific immunoglobulin G (IgG) antibody levels post-vaccination with the current
Streptococcus pneumoniae
human reference serum standard (007sp) for anti-pneumococcal capsule antibodies. Development of new pneumococcal conjugate vaccines (PCVs) with additional serotypes not in 007sp (e.g., V116, a 21-valent PCV) requires a new reference serum. Antibody concentrations to 33 pneumococcal serotypes were assigned in a new Merck Pneumococcal Reference Serum Standard (MPRSS-01; Merck Pneumococcal Reference Serum Standard) using the pneumococcal electrochemiluminescence assay. MPRSS-01 was generated by pooling high-titer serum samples from adults immunized with either 23-valent pneumococcal polysaccharide vaccine (PPSV23) or V116. For the 24 serotypes with established IgG concentrations, the corresponding antibody concentrations in MPRSS-01 were assigned via direct calibration to 007sp, while a cross-standardization approach was used for the nine novel serotypes. Serotype 7F was initially chosen as the reference calibrator for cross-standardization due to parallelism across dilution–response curves demonstrated across all 33 serotypes, and an evaluation of the single-calibrator approach was conducted for the 24 serotypes. Potential systematic bias from using a unique serotype for calibration was identified and addressed by further adjusting the estimated IgG concentrations of the nine novel serotypes. Using the final MPRSS-01 antibody concentration assignments, and the calibration factor relating MPRSS-01 to 007sp, antibody concentration assignments for 007sp were provided for the nine novel serotypes. This proposal was accepted by the Center for Biologics Evaluation and Research (CBER), enabling V116 to bridge old and new human pneumococcal reference sera.

IMPORTANCE

Immunogenicity of pneumococcal vaccines is measured using post-vaccination serotype-specific immunoglobulin G (IgG) antibodies in serum using enzyme-linked immunoassays with the 007sp reference serum containing serotype-specific IgG for 24 pneumococcal serotypes. With the development of next-generation PCVs, a new
S. pneumoniae
reference serum standard was needed to include serotypes beyond the existing 24 in 007sp. In this study, antibody concentrations to 33 pneumococcal serotypes were assigned in a new Merck Pneumococcal Reference Serum Standard (MPRSS-01) using the pneumococcal electrochemiluminescence assay, enabling V116 to maintain the link to the historical human pneumococcal standard reference serum while utilizing the new human pneumococcal reference serum.
Can GastroPanel be used as a triage tool to select patients with advanced atrophic gastritis for gastroscopy? A prospective clinical validation study
Authors: Papadia Cinzia , Marelli Laura , Wood Eleanor , Novelli Marco , Feakins Roger , Syrjänen Kari Juhani , Shidrawi Ray 
Published: January 2025

ObjectiveGastric adenocarcinoma (GAC) is the 17th most common cancer in the UK with a 5-year survival rate of 22%. GastroPanel (Biohit Oyj; Helsinki, Finland) is an ELISA kit that measures pepsinogen I (PGI); pepsinogen II (PGII); gastrin-17 (G-17); and Helicobacter pylori IgG antibodies (Hp IgG). PGI and the PGI/PGII ratio correlate inversely with the severity of chronic atrophic gastritis (AG). The aim of this study was to assess GastroPanel performance in the identification of moderate to severe AG in dyspepsia.MethodsIn this UK, single-centre, prospective diagnostic accuracy study, 324 patients [56.8% (n=184) female; median age 57 years (range 39–92 years)] were recruited for gastroscopy with biopsy and histology according to the updated Sydney System (USS). Blood (plasma) samples were collected for GastroPanel analysis. Paired samples were obtained from 268 patients [56.3% (n=151) female; median age=57 (range 39–92 years)]. GastroPanel results were interpreted using the GastroSoft app (Biohit).ResultsOverall agreement between GastroPanel and the USS classification was 90% (95% CI=86.7 to 93.8%), with a weighted kappa (κw) of 0.828 (95% CI=0.781 to 0.865). In receiver operating characteristics (ROC) curve analysis, using moderate/severe atrophic gastritis of the corpus (AGC2+) as the endpoint, AUC=0.840 (95% CI 0.630 to 1.000) and 0.960 (95% CI 0.907 to 1.000) for PGI and the PGI/PGII ratio, respectively.ConclusionGastroPanel is a reliable dyspepsia triage test distinguishing patients who can be safely treated conservatively from those with moderate to severe corpus atrophic gastritis at high risk of developing GAC.
Evaluating the pathological and clinical implications of errors made by an artificial intelligence colon biopsy screening tool
Authors: Evans Harriet , Sivakumar Naveen , Bhanderi Shivam , Graham Simon , Snead David , Patel Abhilasha , Robinson Andrew 
Published: January 2025

ObjectiveArtificial intelligence (AI) tools for histological diagnosis offer great potential to healthcare, yet failure to understand their clinical context is delaying adoption. IGUANA (Interpretable Gland-Graphs using a Neural Aggregator) is an AI algorithm that can effectively classify colonic biopsies into normal versus abnormal categories, designed to automatically report normal cases. We performed a retrospective pathological and clinical review of the errors made by IGUANA.MethodsFalse negative (FN) errors were the primary focus due to the greatest propensity for harm. Pathological evaluation involved assessment of whole slide image (WSI) quality, precise diagnoses for each missed entity and identification of factors impeding diagnosis. Clinical evaluation scored the impact of each error on the patient and detailed the type of impact in terms of missed diagnosis, investigations or treatment.ResultsAcross 5054 WSIs from 2080 UK National Health Service patients there were 220 FN errors across 164 cases (4.4% of WSI, 7.9% of cases). Diagnostic errors varied from cases of adenocarcinoma to mild inflammation. 88.4% of FN errors would have no impact on patient care, with only one error causing major patient harm. Factors that protected against harm included biopsies being low-risk polyps or diagnostic features were detected in other biopsies.ConclusionMost FN errors would not result in patient harm, suggesting that even with a 7.9% case-level error rate, this AI tool might be more suitable for adoption than statistics portray. Consideration of the clinical context of AI tool errors is essential to facilitate safe implementation.
Motivational influence of virtual reality in physical therapy for children with cerebral palsy: a systematic review protocol
Authors: Mohd Iqbal Huda Aliah , Zanudin Asfarina , Mohd Nordin Nor Azlin 
Published: January 2025

IntroductionEmulating the vast inclusion of advanced technology in everyday practice, the medical world is no exception to its implementation, in particular, virtual reality (VR). Initially, its introduction as a therapy was accompanied by high aspirations and expectations for its growth and potential. Motivation is said to be the most associated benefit; although it is imperative to note that there exists a paucity of research that specifically quantifies its tangible impact on cerebral palsy (CP). There has yet to be a systematic review of the instruments used to precisely measure motivation and examine its association with the benefits of VR for children with cerebral palsy.Methods and analysisThis review will evaluate comparative studies that used VR therapy as part of interventions for children with CP. Qualitative studies, single-case studies, systematic reviews, literature reviews and guideline audits will be excluded. This review will be conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA-P) guidelines. The methodological protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO). The following electronic databases will be used to access related studies published between January 2012 and December 2023: MEDLINE (via EBSCOhost), Web of Science, Scopus, PubMed and Cochrane. The Peer Review of Electronic Search Strategies checklist will be employed to develop database search strategies.Ethics and disseminationThis review does not require ethical approval as it uses secondary data. The findings will be published in a scientific journal. We intend to contribute to the expansion of knowledge concerning the motivational implications of VR on children with CP.PROSPERO registration numberInternational Prospective Register of Systematic Reviews (identification number CRD42023401079).
EXtending Omalizumab Treatment Intervals in patients with Chronic spontaneous urticaria (EXOTIC): protocol of a multicentre, randomised, open-label, non-inferiority trial
Authors: Zhang Ditte Georgina , Sørensen Jennifer Astrup , Ghazanfar Misbah Noshela , Ali Zarqa , Vestergaard Christian , Thyssen Jacob , Egeberg Alexander , Thomsen Simon Francis 
Published: January 2025

IntroductionOmalizumab, an anti-IgE monoclonal antibody, is effective in treating antihistamine-refractory chronic spontaneous urticaria (CSU). However, tapering strategies for omalizumab are currently not well-studied, and patients may be treated longer than needed. Here, we present the rationale and design of the EXtending Omalizumab Treatment Intervals in patients with Chronic spontaneous urticaria trial, a multicentre, randomised, open-label, non-inferiority clinical trial. The objective of this trial is to investigate if patients with well-controlled CSU, achieved by standard treatment of 300 mg omalizumab administered subcutaneously every 4 weeks (Q4W) for 12 weeks, can maintain disease control with every 6 weeks (Q6W) dosing interval.Methods and analysisParticipants who achieve an Urticaria Control Test (UCT) score ≥12 after 12 weeks on omalizumab will be randomised to 300 mg omalizumab treatment Q4W or Q6W. Treatment arms will be followed for a total of 36 weeks. The primary endpoint is the absolute difference in average UCT score between treatment arms at week 36. Blood samples, Weekly Urticaria Activity Score, Chronic Urticaria Quality of Life Questionnaire, Dermatology Life Quality Index and records of side effects and flares will be obtained throughout the study at weeks 0, 12, 24 and 36.Ethics and disseminationThe study has been approved by the Scientific Ethical Committee of the Capital Region in Denmark, the local Data Protection Agency and the Danish Medicines Agency. All study participants must provide written informed consent. The study will be conducted according to the Helsinki Declaration and Good Clinical Practice. Findings will be disseminated through publication in peer-reviewed journals and presented at international conferences.Trial registration numberEU CT no. 2023-506187-14-00, ClinicalTrials.gov:NCT05916937.