bluuebunny/crossref_metadata_2025_split huggingface dataset:
BTN1A1 is a novel immune checkpoint mutually exclusive to PD-L1
Authors: Yoo Stephen , Kim Young Seung , Park Andrew , Wu Chunai , Lin Steven H. , Jung Hyunjin 
Published: January 2023

Abstract
While PD-1/PD-L1 blockade is a potent antitumor treatment strategy, it is effective in only limited subsets of cancer patients, emphasizing the need for the identification of additional immune checkpoints. Butyrophilin 1A1 (BTN1A1) has been reported to exhibit potential immunoregulatory activity, but its ability to function as an immune checkpoint remains to be systematically assessed and the mechanisms underlying such activity have yet to be characterized. Here, BTN1A1 expression was validated in primary tumor tissue samples, and its ability to suppress T cell activation and T cell-dependent tumor clearance was examined. The relationship between BTN1A1 and PD-L1 expression was further characterized, and an anti-BTN1A1 antibody was developed and administered to tumor-bearing mice to test the amenability of this target to immune checkpoint inhibition. BTN1A1 was confirmed to suppress T cell activation in vitro and in vivo. Robust BTN1A1 expression was detected in a range of solid tumor tissue samples, and BTN1A1 expression was mutually exclusive with that of PD-L1 as a consequence of its inhibition of JAK/STAT signaling-induced PD-L1 upregulation. Antibody-mediated BTN1A1 blockade suppressed tumor growth and enhanced immune cell infiltration in syngeneic tumor-bearing mice. Together, these results confirm that BTN1A1 is a bona fide immune checkpoint and viable immunotherapeutic target for the treatment of anti-PD1/PD-L1 refractory or resistant patients, opening new avenues to improving survival outcomes for patients with a range of cancers.
Silent Sinus syndrome- should we be concerned?
Authors: Gupta Ashana , Ganesan Kandasamy 
Published: January 2023

Abstract
In this paper, we report on the radiological findings of silent sinus syndrome. This is significant because silent sinus syndrome can lead to enophthalmos, facial asymmetry and diplopia if left untreated. It is usually diagnosed based on radiological findings. Patients normally present to the head and neck specialty for unrelated concerns and upon taking radiographic imaging, features of silent sinus syndrome can be seen. It is the duty of the surgeon to be aware of sinus abnormalities and inform the patient of such findings. This paper highlights the radiological features clinicians should be aware of. Clinical relevance: Radiologists and head and neck clinicians should be aware of the features of silent sinus syndrome. The syndrome is poorly reported on but radiological features are consistent across case reports. Objectives: The reader should have an awareness of the radiological signs of maxillary sinus pathology and understand features of silent sinus syndrome. They should be able to Include sinus related findings in radiographic report for orbito-facial imaging.
Occipital neuralgia after COVID-19 vaccination: a report of two cases
Authors: Malheiro Sofia , Costa Diogo , Varela Ricardo 
Published: January 2023

Abstract
BACKGROUND
A wide range of neurological complications has been described following the administration of COVID-19 vaccinations, with headache being the most commonly reported neurological adverse effect, with higher incidence after the second dose. The most common headache is a dull pain or migraine like. Still, two cases of trigeminal neuralgia and a case series of 7 patients with cluster headache who were clinically stable and had a new episode a few days after COVID-19 vaccination have also been reported. Herein, we describe the first two cases of occipital neuralgia developing after COVID-19 vaccination.
CASES PRESENTATION
Two cases were observed in the neurology outpatient clinic of a tertiary university referral center in Portugal between October 2021 and June 2022. Both patients developed the headache within 1 to 6 days after the second dose of BNT162b2 (Pfizer). One had no previous history of headaches, and the other had a migraine that was clearly different from the present headache. Each case is described, checked for ICHD criteria for occipital neuralgia, and its temporal relation with COVID-19 Vaccination is reported.
CONCLUSIONS
COVID-19 vaccination can trigger various types of headaches, far beyond the most commonly described feature of dull pain, possibly by activating an immune-inflammatory response.
Novel therapeutic targets identification of Delftia tsuruhatensis strain TR1180 employing subtractive genomics approach
Authors: Mahmood Sajjat , Shihab Md Maksudur Rahman , Rashid Afsara Binte , Imu Ishrat Jahan , Nawer Narmin , Karim Md. Hasanul 
Published: January 2023

Abstract
Delftia tsuruhatensis is an opportunistic pathogen, that causes human infection in immunocompromised individuals. The organism was found to be infectious in the respiratory tract and urinary tract infections. Isolation of D. tshuruhatensis showed resistance to common antibiotics, resulting in an alarming signal from the pathogen. Importantly, the D. tshuruhatensis strain was found to have the property of having IMP-1 Metallo-β-Lactamase, which could hydrolyze β-lactam antibiotics. To combat the challenge of antibiotic resistance, novel drug targets can be effective. For suggesting the novel drug targets, the entire proteome of D. tshuruhatensis strain TR1180 was subjected to subtractive genomic analysis using a variety of bioinformatic tools and servers. To identify human homologue proteins of the pathogen and proteins involved in common metabolic pathways between the pathogen and host, various bioinformatics tools and web servers were used. Only 62 proteins were found to be linked to pathogen-specific pathways; these proteins were then further screened to single out membrane-antigenic proteins that could be targeted by medications or vaccines. The novel therapeutic targets with the highest level of antigenicity were discovered to be ‘Nitrate transporter’ and ‘Phospho-N-acetylmuramoyl-pentapeptide-transferase’.
The collagen enhancement by spirulina extract in intrinsic and extrinsic skin aging in albino rat
Authors: Sharafeldein Khaled , Ayesh Hanan , Salama Safwat , Marei Azza 
Published: January 2023

Abstract
The aging of the skin is considered a cumulative process that is classed as intrinsic or extrinsic. Environmental factors like sun exposure and air pollution are considered the main cause of extrinsic aging. Mainly, intrinsic aging reflects the genetic background and depends on time. Spirulina platensis unicellular blue-green algae have a variety of biological and nutritional activities because of their high level of nutrients. The current study aims to investigate the mechanism by which spirulina extract (SE) may act anti-aging in female albino rats. Animals were received SE only, UV radiation only and both. In addition, two intrinsic groups as control old and old rats received SE. Spirulina extract (20mg/mL) was injected subcutaneously before UVA irradiation (2.16 J/cm2), daily for 7 days. The epidermal thickness and the collagen fibers layer were stained utilizing Hematoxylin and Eosin and Masson. There were improvements in the activities of antioxidants including superoxide dismutase (SOD) and reduced glutathione (GSH), downregulating in expressions of inflammatory cytokines; Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). As well as the recovery of collagen density, and reducing the production of matrix metalloproteinases were presented. The results found that spirulina extract may delay the signs of skin aging by enhancing collagen production as well as antioxidant activities and inhibiting collagen degradation and inflammation.
Linguistic profile in children with a double diagnosis of attention deficit and hyperactivity disorder and developmental language disorder
Authors: Moraleda-Sepúlveda Esther , López-Resa Patricia , Pulido-García Noelia 
Published: January 2023

Abstract
Recently, comorbidity and the number of double diagnosis cases of attention deficit hyperactivity disorder (ADHD) and developmental language disorder (DLD) have increased. This study aimed to know the linguistic profile that the children of such cases present. Our sample included 24 participants between 6–11 years old, divided into three groups: ADHD; DLD; double diagnosis of ADHD and DLD. We administered the Celf-4 Language Test with the areas: core language, receptive language, expressive language, language content, language memory and working memory. Our results revealed that the children with double diagnosis performed better than individuals with DLD in Core Language, Expressive Language and Language Memory, but worse than the children with ADHD. These data demonstrate that the presence of DLD determines linguistic differences. The coexistence of ADHD does not predispose to having a different linguistic profile.
Studies of Distribution and Drug Resistance of Biofilm Bacteria and the Influencing Factors of Upper Urinary Calculi Patients with Double J Stent Indwelling
Authors: Chen Qian , Ye JunBing , Zeng Ke , Li Xiao Bin , Zeng Shiping 
Published: January 2023

Abstract
Objective: To analyze the distribution and drug resistance of biofilm bacteria infected with upper urinary calculi patients with double J stent indwelling and its influencing factors.
Methods: A total of 400 patients with upper urinary calculi who adopted double J stent inserting in our hospital from January 2019 to January 2022 were included. The urine and double J stent samples were collected and pathogen culture were performed, and the drug sensitivity test analysis were carried out for isolates.Univariate and multivariate logistic regression analyses were used to analyze the influencing factors of patients with double J-stent associated biofilm bacteria infections.
Results: A total of 13 strains (3.2%) of biofilm bacteria were detected from urine samples and 168 strains (42%) from double J-stent samples (P < 0.05), 95 strains (23.7%) of pathogenic bacteria were separated from urine samples and 117 strains (29.2%) from double J-stent samples (P > 0.05). Escherichia coliwere the most common bacteria. there were significantly higher drug resistance was observed in biofilm bacteria versus urine-cultured pathogens (P < 0.05). Advanced age, long-term catheterization, insufficient water intake, hypoproteinemia, abnormal renal function and diabetes mellitus were independent risk factors for biofilm bacteria infection associated with double J stent(P < 0.05).
Conclusion: Among the upper urinary calculi patients with double J stent indwelling, the positive rate and drug resistance of biofilm bacteria obtained from double-J stent were significantly higher than that from urine.Deem that rational choice of antibiotics and appropriate treatment led to better prognosis.
Volume monitoring of the milling tool tip wear and breakage based on multi-focus image three-dimensional reconstruction
Authors: Peng Yeping , Qin Shucong , Wang Tao , Hu Yixi , Nie Shiping 
Published: January 2023

Abstract
In precision machining, the milling tool’ geometry has a great influence on the milled surface quality. The research on milling tool state monitoring was mainly based on one-dimensional signals and two-dimensional images, which could indirectly obtain the tool state and wear area, but it could not provide the volume of milling tool wear and breakage area, thereby making it difficult to achieve quantitative analysis tool wear. This paper proposed a three-dimensional (3D) reconstruction method of the milling tool tip, it could build a 3D model of the milling tool tip, and then the volume of the wear and breakage region of the milling tool tip was extracted by the 3D model. Firstly, the focusing degree of image sequence’s pixels was calculated based on the non-subsampled discrete shearlet transform (NSST) and Laplace algorithm, and the 3D reconstruction of the milling tool tip was completed according to the shape-from-focus (SFF) principle; secondly, the depth values were optimized by fitting the focusing degree curve of pixels in the image sequence with Gaussian function; finally, the volume of the 3D point cloud of the milling tool tip was calculated by the Simpson double numerical integration method, and the material loss in the damaged region could be obtained. In the 3D reconstruction experiment of the milling tool tip, comparing the different focus degree evalution operators of SFF, the 3D point cloud obtained by this paper's proposed 3D reconstruction method has the least noise and the best performance in the root-mean-square error, correlation, and smoothness indexes. In addition, compared with Genmagic software, the 3D point cloud volume calculation method adopted in this paper could accurately calculate the 3D point cloud volume of the milling tool tip, and the percentage error was less than 1%.
A novel wheel path generation approach for grinding taper end-mills subject to adaptive cross-section
Authors: Wang Liming , Yang Jianping , Fang Yang , Zhu Jiang , Li Jianfeng 
Published: January 2023

Abstract
Taper end-mills are widely used in CNC machining and the flute are crucial to the cutting performance of the end-mill. However, it is very difficult to form the wheel path of grinding taper end-mill due to its complex structure and variable cross-section. In the traditional methods, the wheel path is generated by experience, and the accuracy of flute parameters cannot be guaranteed. To overcome those problems, this paper proposed a wheel path generation approach for taper end-mills flute-grinding subject to adaptive cross-section, which is capable to ensure the accuracy of parameters of variable cross-section. In the wheel path generation, the taper end-mill is discretized into a series of finite slices, which can be transformed into solving a series sub-problem of grinding cylindrical end-mills. To eliminate the interference and discontinuity caused by the discretization, the enhancement based on sensitivity analysis and smoothing method are used to optimize the grinding wheel path. Finally, the approach is validated by both simulation and experiments. The results indicated that this approach is capable to generate the wheel path for grinding the taper end-mill flute accurately and efficiently subject to the adaptive cross-section parameters.
Scent detection of Brucella abortus by African giant pouched rats (Cricetomys ansorgei).
Authors: Mwampashi Raphael , Cutright Ellie , Fast Cynthia D. , Bonfoh Bassirou , Kazwala Rudovick R. , Mathew Coletha 
Published: January 2023

Abstract

Background Brucellosis is a contagious zoonosis caused by Brucella bacterium. While the disease has been eradicated in most developed countries, it remains endemic in sub–Saharan Africa where access to reliable diagnostics is limited. African giant pouched rats (Cricetomys ansorgei) have been trained to detect the scent of Mycobacterium tuberculosis to increase case detection in sub-Saharan Africa. Given the similar diagnostic challenges facing brucellosis and tuberculosis, we explored the feasibility of training African giant pouched rats to detect Brucella.
Results After 3 months of training, rats reliably identified cultured Brucella, achieving an average sensitivity of 93.56% (SD = 0.650) and specificity of 97.65% (SD = 0.016). Rats readily generalized to novel, younger Brucella cultures that presumably generated a weaker volatile signal and correctly identified at least one out of three fecal samples spiked with Brucella culture during a final test of feasibility.
Discussion To our knowledge, these experiments are the first to demonstrate Brucella emits a unique odor profile that scent detection animals can be trained to identify. Importantly, cultured E. colisamples were included throughout training and test to ensure the rats learned to specifically identify Brucella bacteria rather than any bacteria in comparison to bacteria-free culture medium. E. coli controls therefore served a crucial function in determining to what extent Brucella abortus emits a unique odor signature. Further research is needed to determine if a Brucella-specific volatile signature is present within clinical samples. If confirmed, the present results suggest trained rats could serve as a valuable, novel method for the detection of Brucellainfection.
Synopsis of myxosporean species (Cnidaria: Myxozoa) parasitizing fishes from Vietnam
Authors: Chinh Nguyen Ngoc , Ha Nguyen Thi Hoang , Doanh Pham Ngoc , Eiras J. C. , Whipps Christopher M. , Shirakashi Sho 
Published: January 2023

Abstract
This paper provides an update checklist of species-level identified myxosporean species from marine and freshwater fishes in Vietnam. The list includes 51 nominal myxosporean species (38 marine and 13 freshwater) belonging to 9 genera: Myxobolus (26 species); Kudoa (6 species); Henneguya (6 species); Thelohanellus (5 species); Unicapsula (2 species); Ceratomyxa (2 species), Zschokkella (2 species); Auerbachia (1 species), and Meglitschia (1 species). For each parasite species, information on morphological characteristics of myxospore, fish hosts, infection sites, and collection locality in Vietnam along with GenBank accession number of nucleotide sequence data and the line drawings were provided. In addition, taxonomic status of several species was discussed and Myxobolus eszterbaueri nom. nov. is proposed as a junior homonym for Myxobolus hakyi Baska, Voronin, Eszterbauer, Müller, Marton & Molnár 2009.
Characteristics and phylogenetic analysis of the complete chloroplast genome of Viburnum japonicum (Adoxaceae)
Authors: Zhu Hong , Liu Juan , li He-Peng , Yue Chun-lei , Gao Mei-Rong 
Published: January 2023

Abstract
Viburnum japonicum (Thunb.) Sprengel is a unique and endangered species in the family Adoxaceae and is endemic to the coastal region of Eastern Asia (China, Japan, and South Korea). However, its systematic position remains controversial. Here, we characterized and reported a second complete chloroplast genome of V. japonicum using the Illumina NovaSeq platform. The total length of the chloroplast genome is 158,606 bp with G + C contents of 38.08%, including a large single-copy (LSC) region, a small single-copy (SSC) region, and a pair of inverted repeats (IRs) regions with lengths of 87,060 bp, 18,510 bp, and 26,516 bp, respectively. Phylogenetic analysis revealed that the two accessions of V. japonicum were close related to a clade of V. setigerum, V. erosum, and V. fordiae within the genus Viburnum.
Transcriptome and Metabolome Integrated Analysis Reveals the mechanism of Cinnamomum bodinieri root response to alkali stress
Authors: Han Haozhang , Zhang Lihua , Li Suhua , Zhao Rong , Wang Fang , Dong Rong , Wang Xiaoli 
Published: January 2023

Abstract
Cinnamomun bodinieri's normal growth and development are hampered by alkali stress, impeding its production and application of Cinnamomum bodinieri. The root organs being in direct contact with the cultivation environment, are sensitive to environmental stress. The present study revealed the differentially expressed genes and differentially metabolized products of Cinnamomum bodinieri root under alkali stress employing transcriptome and metabonomic analysis. The findings revealed that 690 differentially expressed genes and 269 metabolites were significantly different among HT6 and HCK6. Similarly, 1000 differentially expressed genes and 360 metabolites with significant differences were identified in HT48.vs.HCK48 combination. The combined analysis of transcriptome and metabolome identified 9 metabolic pathways at 6h and 48h after alkali treatment, including the biosynthesis pathway of tropane, piperidine and pyridine alkaloids, pyrimidine metabolic pathway, phenylalanine metabolic pathway, isoquinoline alkaloid biosynthesis pathway, glycolysis/gluconeogenesis pathway, flavonoid biosynthesis pathway, fatty acid biosynthesis pathway, carbon fixation pathway in photosynthetic organisms, the metabolic pathway of amino sugar and nucleotide sugar. Therefore, the strategy of Cinnamomum bodinieri to cope with alkali stress may be to increase osmotic regulation and antioxidant activity by accumulating alkaloids, flavonoids secondary metabolites, and N-acetyl-L-phenylalanine, ensure the stability of cell structure and function through the accumulation of lauric acid and palmitic acid, provide energy for plants to withstand alkali stress by accelerating the glycolysis process, and improve plants' resistance to biological and abiotic stress by inducing the activity of chitinase, The accumulation of oxaloacetic acid and other organic acids alleviates alkali stress environment. This study provides support for the analysis of the pathways and regulatory networks of Cinnamomum bodinieri in response to alkali stress.
Effect of Exogenous Natural Plant Growth Regulators (Pgrs) on the Morphology, Growth, and Nutrient of Sea Grapes (Caulerpa Racemosa)
Authors: Windarto Seto , Susilowati Titik , Haditomo Alfabetian Harjuno Condro , Harwanto Dicky 
Published: January 2023

Abstract

This study aimed to determine the effectiveness of aqueous extract from various plants as an exogenous plant growth regulator (PGRs) in the morphology, growth, and nutrition of C. racemosa. The natural PGRs used were coconut water (CW), shallots and coconut water (AESCW), maize (AEM), moringa leaves and mung beansprout (AEMBS), and E. cottonii (AEEC). A 50g of C. racemosa was soaked on the natural PGRs and was cultivated for 30 days with a sandy mud substrate. The results showed that the soaking of C. racemosa on natural PGRs had a significant effect (P<0.05) on absolute growth, specific growth rate, and nutrient. C. racemosa soaked in aqueous shallot and coconut water showed the best absolute growth (214.32±7.68 g) and SGR (5.55±0.08%/day), meanwhile C. racemosa soaked in coconut water had the best nutrition (protein: 9.54±0.16%; fibre: 1.37±0.21%; and fat 0.42±0.04%). The morphological analysis showed that soaking C. racemosa on natural PGRs resulted in long fronds, bigger stolons, a greener and fresh colour, and more ramulli.
Influence of the hierarchical architecture of multi-core iron oxide nanoflowers on their magnetic properties
Authors: Neumann Stefan , Kuger Laura , Arlt Carsten-Rene , Franzreb Matthias , Rafaja David 
Published: January 2023

Abstract
Magnetic properties of superparamagnetic iron oxide nanoparticles are controlled mainly by their particle size and by their particle size distribution. Magnetic properties of multi-core iron oxide nanoparticles, often called iron oxide nanoflowers (IONFs), are additionally affected by the interaction of magnetic moments between neighboring cores. The knowledge about the hierarchical structure of IONFs is therefore essential for understanding the magnetic properties of IONFs. In this contribution,the architecture of multi-core IONFs was investigated using correlative multiscale transmission electron microscopy (TEM), X-ray diffraction and dynamic light scattering. The multiscale TEM measurements comprised low-resolution and high-resolution imaging as well as geometric phase analysis. The IONFs contained maghemite with the average chemical composition γ-Fe2.72±0.02O4. The metallic vacancies located on the octahedral lattice sites of the spinel ferrite structure were partially ordered. Individual IONFs consisted of several cores showing frequently a specific crystallographic orientation relationship between direct neighbors. This oriented attachment may facilitate the magnetic alignment within the cores. Individual cores were composed of partially coherent nanocrystals having almost the same crystallographic orientation. The sizes of individual constituents revealed by the microstructure analysis were correlated with the magnetic particle sizes that were obtained from fitting the measured magnetization curve by the Langevin function.