bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identificación de espermatozoides mediante tinciones Diff Quick, Gram, Panóptico y Papanicolau y su posible aplicación en la Investigación Forense
Authors: Cabezas Arévalo Mónica Raquel , Chango Gadvay Marco Vinicio 
Published: June 2023

Introducción: En la actualidad hay un incremento de los delitos sexuales en el país, y muchos quedan en la impunidad porque no han sido reportados o lo hacen de manera tardía, ya que las víctimas tienen temor a denunciar el hecho para no ser revictimizadas durante el proceso legal. El trabajo pericial en biología forense inicia con la toma de muestra de la víctima y el análisis de muestras biológicas, se centra en la investigación de semen o líquido espermático, que se pudieran haber dejado tanto en la víctima como en el lugar del hecho, y se continua con la búsqueda microscópica para visualizar espermatozoides utilizando la tinción específica "Christmas Tree o árbol de navidad". Metodología: El propósito de la investigación fue validar las tinciones Diff Quick, Gram, Panóptico y Papanicolau como técnicas alternativas para la identificación de espermatozoides y su posible aplicación en la investigación forense. El diseño fue experimental ya que se manipulan intencionalmente los parámetros de prueba y compara las tinciones descritas con la prueba Gold Standard Christmas Tree. Las muestras de líquido seminal pertenecen a pacientes voluntarios que acuden al Hospital General San Juan Riobamba, en el periodo septiembre – octubre 2022. Resultados: Los resultados estuvieron en función de la calidad de imagen microscópica que permitieron establecer las ventajas y desventajas de cada una de las pruebas. Se encontró diferencia estadística significativa entre la sensibilidad de las pruebas experimentales (p-valor < 0,05). Las tinciones Diff Quick y Panóptico mostraron mayor sensibilidad en la identificación de espermatozoides, en estas tinciones además se observa un mayor tamaño de la cabeza del espermatozoide y mostraron un fondo extendido ligeramente sucio en comparación con las demás tinciones. Conclusiones: Las pruebas experimentales evidencian mayor sensibilidad en las tinciones Diff Quick y Panóptico, pero Gram y Papanicolau podrían usarse con este propósito porque se alcanza buena definición morfológica de los espermatozoides. Área de estudio general: Criminalística. Área de estudio específica: Biología Forense.
SENTIMENT ANALYSIS WITH A CASE STUDY OF PRACTICE CARD ON TWITTER SOCIAL MEDIA USING NAIVE BAYES METHOD
Authors: Asthakhuroh Asthakhuroh , Komarudin Rachman , Kholifah Desiana Nur 
Published: June 2023

-In early March 2022, Indonesia experienced a coronavirus pandemic which caused COVID-19 to enter for the first time. Since then, all sectors have been affected by the COVID-19 pandemic, not only health, the economic sector has also been seriously affected by this pandemic. In overcoming employment problems, the government makes a policy of the Pre-Employment Card program. The Pre-Employment Card Program is one of the government's efforts to expand job opportunities and to increase competitiveness which later became one of the social assistance for the community to overcome the Covid-19 pandemic. The implementation of the Pre-Employment Card program received pros and cons from the community, one of which was on Twitter social media. The results of the sentiment analysis of the pre-employment card program are mostly positive. The test results show that the Naïve Bayes Classifier method is successful in classifying sentiment with the highest accuracy value of 96%, the highest precision value of 98%, and the highest recall value of 96%, and AUC of 96%.
DIGITAL IMAGE IDENTIFICATION OF PLANKTON USING REGIONPROPS AND BAGGING DECISION TREE ALGORITHM
Authors: Hikmatulloh Hikmatulloh , Rianto Yan , Anton Anton 
Published: June 2023

Peranan plankton sangat penting bagi kehidupan organisme disekitarnya, sehingga penelitian prihal plankton sangatlah dibutuhkan karena kaitannya dengan kelangsungan kehidupan mahluk hidup lainnya. Kendala yang sering didapatkan dalam hal penelitian plankton khususnya dalam hal pengidentifikasian plankton yaitu tidak efisiennya dalam aspek waktu dan organisme ini memiliki ukuran rata-rata yang sangat kecil. Dalam hal ini diperlukan alternatif yang lebih baik dalam pengidentifikasian jenis plankton ini dengan cara pemrosesan gambar pada citra plankton secara digital atau biasa disebut dengan istilah “Digital Image Processing”. Penelitian ini bertujuan untuk melakukan pengolahan citra digital plankton sebanyak 144 citra yang yang dibagi menjadi 75% sebagai data pelatihan dan 25% sebagai data pengujian, dan citra tersebut didapatkan dari riset pada yayasan Kanopi Indonesia. Dalam prosesnya citra ini dianalisa bentuk menggunakan fungsi Regionprops sehingga didapatkan fitur pembeda dari masing-masing jenis plankton. Setelah citra terekstraksi fitur nya selanjutnya dilakukan pengolahan data dengan mengklasifikasikan setiap jenis plankton tersebut. Untuk menghasilkan sebuah klasifikasi data yang lebih baik, dalam penelitian ini menggunakan algoritma Bagging Decision Tree dalam pengolahan data nya dan menghasilkan akurasi sebesar 92.59%. Algoritma Bagging Decision Tree ini cukup baik dan mudah untuk di implemntasikan kedalam sebuah program identifikasi jenis plankton, terbukti dengan pengujian pada data citra pengujian menghasilkan 33 citra teridentifikasi dengan benar dari total pengujian sebanyak 36 citra.
Cholesterol-lowering Effect of Soy Nuts and Tempeh on Hypercholesterolemic Subjects
Authors: Zulaikha Hermawati Nur , Dijaya Muliadi Rendy , Kartawidjajaputra Felicia , Antono Lina 
Published: June 2023

Exploration towards food with cholesterol-lowering property would be beneficial to reduce the incidents of cardiovascular caused by increasing number of people with hypercholesterolaemia. As much as 42 participants with total cholesterol (TC) levels ≥4.92 mmol/L were studied in a three-arms parallel intervention trial. As much as 72g soy nuts and 66g tempeh were consumed daily (25g soy protein/day) for six weeks, while control group was not given any sample. TC level and body composition were measured before and after the treatment for all groups. In soy nuts group, lower TC level was observed (-0.85±0.82 mmol/L, p<0.05) compared to the control group. Meanwhile in tempeh group, non-significant lower TC level was also observed (-0.40±1.19 mmol/L). Differences in body composition parameters were also measured and resulted in significant lower body weight, fat, and visceral fat in soy nuts group (p<0.05) while in tempeh group, only body weight and waist circumference were significantly decreased (p<0.05). This study suggested that daily consumption of soy nuts and tempeh containing 25g soy protein for six weeks showed a tendency to lower TC levels in hypercholesterolaemic-Indonesian participants. A more significant effect might be observed in subject with higher TC levels; and thus, further study is encouraged.
An automated system for polymer wear debris analysis in total disc arthroplasty using convolution neural network
Authors: Kandel Sushil , Su Steven , Hall Richard M. , Tipper Joanne L. 
Published: June 2023

Introduction: Polymer wear debris is one of the major concerns in total joint replacements due to wear-induced biological reactions which can lead to osteolysis and joint failure. The wear-induced biological reactions depend on the wear volume, shape and size of the wear debris and their volumetric concentration. The study of wear particles is crucial in analysing the failure modes of the total joint replacements to ensure improved designs and materials are introduced for the next generation of devices. Existing methods of wear debris analysis follow a traditional approach of computer-aided manual identification and segmentation of wear debris which encounters problems such as significant manual effort, time consumption, low accuracy due to user errors and biases, and overall lack of insight into the wear regime.Methods: This study proposes an automatic particle segmentation algorithm using adaptive thresholding followed by classification using Convolution Neural Network (CNN) to classify ultra-high molecular weight polyethylene polymer wear debris generated from total disc replacements tested in a spine simulator. A CNN takes object pixels as numeric input and uses convolution operations to create feature maps which are used to classify objects.Results: Classification accuracies of up to 96.49% were achieved for the identification of wear particles. Particle characteristics such as shape, size and area were estimated to generate size and volumetric distribution graphs.Discussion: The use of computer algorithms and CNN facilitates the analysis of a wider range of wear debris with complex characteristics with significantly fewer resources which results in robust size and volume distribution graphs for the estimation of the osteolytic potential of devices using functional biological activity estimates.
The mitochondrial Ca2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferation
Authors: Fernandez Garcia Emily , Paudel Usha , Noji Michael C. , Bowman Caitlyn E. , Rustgi Anil K. , Pitarresi Jason R. , Wellen Kathryn E. , Arany Zolt , Weissenrieder Jillian S. , Foskett J. Kevin 
Published: June 2023

Introduction: The mitochondrial uniporter (MCU) Ca2+ ion channel represents the primary means for Ca2+ uptake by mitochondria. Mitochondrial matrix Ca2+ plays critical roles in mitochondrial bioenergetics by impinging upon respiration, energy production and flux of biochemical intermediates through the TCA cycle. Inhibition of MCU in oncogenic cell lines results in an energetic crisis and reduced cell proliferation unless media is supplemented with nucleosides, pyruvate or α-KG. Nevertheless, the roles of MCU-mediated Ca2+ influx in cancer cells remain unclear, in part because of a lack of genetic models.Methods: MCU was genetically deleted in transformed murine fibroblasts for study in vitro and in vivo. Tumor formation and growth were studied in murine xenograft models. Proliferation, cell invasion, spheroid formation and cell cycle progression were measured in vitro. The effects of MCU deletion on survival and cell-death were determined by probing for live/death markers. Mitochondrial bioenergetics were studied by measuring mitochondrial matrix Ca2+ concentration, membrane potential, global dehydrogenase activity, respiration, ROS production and inactivating-phosphorylation of pyruvate dehydrogenase. The effects of MCU rescue on metabolism were examined by tracing of glucose and glutamine utilization for fueling of mitochondrial respiration.Results: Transformation of primary fibroblasts in vitro was associated with increased MCU expression, enhanced MCU-mediated Ca2+ uptake, altered mitochondrial matrix Ca2+ concentration responses to agonist stimulation, suppression of inactivating-phosphorylation of pyruvate dehydrogenase and a modest increase of mitochondrial respiration. Genetic MCU deletion inhibited growth of HEK293T cells and transformed fibroblasts in mouse xenograft models, associated with reduced proliferation and delayed cell-cycle progression. MCU deletion inhibited cancer stem cell-like spheroid formation and cell invasion in vitro, both predictors of metastatic potential. Surprisingly, mitochondrial matrix [Ca2+], membrane potential, global dehydrogenase activity, respiration and ROS production were unaffected. In contrast, MCU deletion elevated glycolysis and glutaminolysis, strongly sensitized cell proliferation to glucose and glutamine limitation, and altered agonist-induced cytoplasmic Ca2+ signals.Conclusion: Our results reveal a dependence of tumorigenesis on MCU, mediated by a reliance on MCU for cell metabolism and Ca2+ dynamics necessary for cell-cycle progression and cell proliferation.
Antibacterial and anti-inflammatory effects of PGLa-loaded TiO2 nanotube arrays
Authors: Xuan Bin , Li Lei , Zhang Hui , Liu Zhuojue , Luo Ruxi , Yang Wenpeng , Wang Weili 
Published: June 2023

Objectives: This study investigated the antimicrobial effect and anti-inflammatory activities of PGLa-loaded TiO2 nanotube arrays (TiO2 NTs) in osteoblast-like MG-63 cells.Methods: The surface morphology and roughness of three titanium (Ti) substrates (Ti, TiO2 NTs, PGLa-loaded TiO2 NTs) were evaluated by scanning electron microscopy (SEM) and atomic force microscope (AFM). The wettability of three titanium substrates was evaluated by contact angle. Biocompatibility of PGLa-loaded TiO2 NTs were evaluated in MG-63 cells (cell adhesion, proliferation, cytoskeletal evaluation and alkaline phosphatase activity). Spread plate counting method was used to evaluate antibacterial abilities of the titanium substrates. The calcein AM/PI staining evaluated cell viability of MG-63 cells on the substrates with or without proinflammatory factors (TNF-α).Results: The average surface roughness of untreated Ti, TiO2 NTs, PGLa-loaded TiO2 NTs were found to be 135.8 ± 6.4 nm, 300.5 ± 10.5 nm, 348.9 ± 16.9 nm, respectively. The contact angle of the untreated Ti was 77.4° ± 6.6°. TiO2 NTs displayed excellent wettability which of contact angle was 12.1° ± 2.9°. The contact angle of the PGLa-loaded TiO2 NTs was 34.6° ± 4.9°. MG-63 cells on surface of PGLa-loaded TiO2 NTs showed better cell adhesion, proliferation and osteogenic activity. The antibacterial rate of PGLa-loaded TiO2 NTs group significantly increased (84.6% ± 5.5%, p &lt; 0.05). The rate of dead cells on the surfaces of the PGLa-loaded TiO2 NTs with TNF-α decreased significantly (4.49% ± 0.02, p &lt; 0.01).Conclusion: PGLa-loaded TiO2 NTs have multi-biofunctions including biocompatibility, antibacterial and anti-inflammatory properties.
Validation of miRNAs as diagnostic and prognostic biomarkers, and possible therapeutic targets in medullary thyroid cancers
Authors: Mondin Alberto , Bertazza Loris , Barollo Susi , Pedron Maria Chiara , Manso Jacopo , Piva Ilaria , Basso Daniela , Merante Boschin Isabella , Iacobone Maurizio , Pezzani Raffaele , Mian Caterina , Censi Simona 
Published: June 2023

IntroductionMedullary thyroid cancer (MTC) is a rare type of neuroendocrine tumor that produces a hormone called calcitonin (CT). Thyroidectomy is the preferred treatment for MTC, as chemotherapy has been shown to have limited effectiveness. Targeted therapy approaches are currently being used for patients with advanced, metastatic MTC. Several studies have identified microRNAs, including miR-21, as playing a role in the development of MTC. Programmed cell death 4 (PDCD4) is a tumor suppressor gene that is an important target of miR-21. Our previous research has shown that high levels of miR-21 are associated with low PDCD4 nuclear scores and high CT levels. The aim of this study was to investigate the potential of this pathway as a novel therapeutic target for MTC.MethodsWe used a specific process to silence miR-21 in two human MTC cell lines. We studied the effect of this anti-miRNA process alone and in combination with cabozantinib and vandetanib, two drugs used in targeted therapy for MTC. We analyzed the effect of miR-21 silencing on cell viability, PDCD4 and CT expression, phosphorylation pathways, cell migration, cell cycle, and apoptosis.ResultsSilencing miR-21 alone resulted in a reduction of cell viability and an increase in PDCD4 levels at both mRNA and protein levels. It also led to a reduction in CT expression at both mRNA and secretion levels. When combined with cabozantinib and vandetanib, miR-21 silencing did not affect cell cycle or migration but was able to enhance apoptosis.ConclusionSilencing miR-21, although not showing synergistic activity with TKIs (tyrosine kinase inhibitors), represents a potential alternative worth exploring as a therapeutic target for MTC.
Postoperative adjuvant tyrosine kinase inhibitors combined with anti-PD-1 antibodies improves surgical outcomes for hepatocellular carcinoma with high-risk recurrent factors
Authors: Li Jian , Wang Wen-qiang , Zhu Rong-hua , Lv Xing , Wang Jin-lin , Liang Bin-yong , Zhang Er-lei , Huang Zhi-yong 
Published: June 2023

BackgroundThe clinical value of postoperative adjuvant therapy (PAT) for hepatocellular carcinoma (HCC) remains unclear. This study aimed to explore the effect of PAT with tyrosine kinase inhibitors (TKIs) and anti-PD-1 antibodies on the surgical outcomes of HCC patients with high-risk recurrent factors (HRRFs).MethodsHCC patients who underwent radical hepatectomy at Tongji Hospital between January 2019 and December 2021 were retrospectively enrolled, and those with HRRFs were divided into PAT group and non-PAT group. Recurrence-free survival (RFS) and overall survival (OS) were compared between the two groups after propensity score matching (PSM). Prognostic factors associated with RFS and OS were determined by Cox regression analysis, and subgroup analysis was also conducted.ResultsA total of 250 HCC patients were enrolled, and 47 pairs of patients with HRRFs in the PAT and non-PAT groups were matched through PSM. After PSM, the 1- and 2-year RFS rates in the two groups were 82.1% vs. 40.0% (P &lt; 0.001) and 54.2% vs. 25.1% (P = 0.012), respectively. The corresponding 1- and 2-year OS rates were 95.4% vs. 69.8% (P = 0.001) and 84.3% vs. 55.5% (P = 0.014), respectively. Multivariable analyses indicated that PAT was an independent factor related to improving RFS and OS. Subgroup analysis demonstrated that HCC patients with tumor diameter &gt; 5 cm, satellite nodules, or vascular invasion could significantly benefit from PAT in RFS and OS. Common grade 1-3 toxicities, such as pruritus (44.7%), hypertension (42.6%), dermatitis (34.0%), and proteinuria (31.9%) were observed, and no grade 4/5 toxicities or serious adverse events occurred in patients receiving PAT.ConclusionsPAT with TKIs and anti-PD-1 antibodies could improve surgical outcomes for HCC patients with HRRFs.
Biotransformation of gallate esters by a pH-stable tannase of mangrove-derived yeast Debaryomyces hansenii
Authors: Song Lei , Wang Xiao-Chen , Feng Zhen-Quan , Guo Yan-Feng , Meng Guo-Qing , Wang Hai-Ying 
Published: June 2023

Introduction: Tannase is a crucial enzyme that finds wide applications in the pharmaceutical industry, feed processing, and beverage manufacturing. Although extensive studies have been conducted on tannases from fungi and bacteria, reports on tannases exhibiting favorable pH stability are relatively limited.Methods: In this study, a tannin-degrading strain Debaryomyces hansenii was screened to induce tannase production, and the corresponding tannase coding gene TANF was successfully cloned and expressed in Yarrowia lipolytica. SDS-PAGE analysis revealed that the purified TanF tannase had a molecular weight of approximately 70 kDa.Results and Discussion: The enzyme demonstrated optimal activity at 40°C and retained over 80% of its activity in the range of 35°C–60°C. Of particular interest, TanF exhibited remarkable enzyme activity at pH 5.0 and retained more than 70% of its relative activity across a wide pH range of 3.0–8.0. Furthermore, TanF exhibited broad substrate specificity for gallate esters. The final gallic acid production by TanF from tannic acid achieved 18.32 g/L. Therefore, the excellent properties TanF has been demonstrated to be an efficient tool for the preparation of gallic acid.
Long-term effects of the gait treatment using a wearable cyborg hybrid assistive limb in a patient with spinal and bulbar muscular atrophy: a case report with 5 years of follow-up
Authors: Iijima Kensuke , Watanabe Hiroki , Nakashiro Yuichi , Iida Yuki , Nonaka Michio , Moriwaka Fumio , Hamada Shinsuke 
Published: June 2023

BackgroundSpinal and bulbar muscular atrophy (SBMA) is a progressive neuromuscular degenerative disease characterized by the degeneration of lower motor neurons in the spinal cord and brainstem and neurogenic atrophy of the skeletal muscle. Although the short-term effectiveness of gait treatment using a wearable cyborg hybrid assistive limb (HAL) has been demonstrated for the rehabilitation of patients with SBMA, the long-term effects of this treatment are unclear. Thus, this study aimed to investigate the long-term effects of the continued gait treatment with HAL in a patient with SBMA.ResultsA 68-year-old man with SBMA had lower limb muscle weakness and atrophy, gait asymmetry, and decreased walking endurance. The patient performed nine courses of HAL gait treatment (as one course three times per week for 3 weeks, totaling nine times) for ~5 years. The patient performed HAL gait treatment to improve gait symmetry and endurance. A physical therapist adjusted HAL based on the gait analysis and physical function of the patient. Outcome measurements, such as 2-min walking distance (2MWD), 10-meter walking test (maximal walking speed, step length, cadence, and gait symmetry), muscle strength, Revised Amyotrophic Lateral Sclerosis Functional Assessment Scale (ALSFRS-R), and patient-reported outcomes, were evaluated immediately before and after gait treatment with HAL for each course. 2MWD improved from 94 m to 101.8 m, and the ALSFRS-R gait items remained unchanged (score 3) for approximately 5 years. The patient could maintain walking ability in terms of gait symmetry, walking endurance, and independence walking despite disease progression during HAL treatment.ConclusionThe long-term gait treatment with HAL in a patient with SBMA may contribute to the maintenance and improvement of the gait endurance and ability to perform activities of daily living. The cybernics treatment using HAL may enable patients to relearn correct gait movements. The gait analysis and physical function assessment by a physical therapist might be important to maximize the benefits of HAL treatment.