bluuebunny/crossref_metadata_2025_split huggingface dataset:
Centering Girls' (Media-Making) Stories: A Pandemic Exploration of Video-Storytellers and their Practices, Personas, and Projects
Authors: Terzopoulos Tatyana 
Published: May 2023

<p>This interdisciplinary, feminist-informed research explores racialized tween and teen girls’ video-based storytelling and considers how extracurricular programs can support their media-making. Drawing from youth media cultures and media education scholarship, this work aligns with and builds upon research about community-based youth documentary media-making initiatives and limited yet pivotal scholarship that centres girls’—marginalized girls in particular—experiences, including as media-makers. It was further motivated by the prevailing lack of diverse representation in key creative and leadership roles in media industries, my experiences as a woman working in media, and the paucity of research on Canadian youth and their experiences learning about and making media.</p>
<p>My inquiry was underpinned by feminist theory, public pedagogy, and feminist media. Utilizing a qualitative case study design, I designed and facilitated a virtual digital storytelling program in Spring 2021 of the pandemic; four ethnoracially-diverse and marginalized girl-identifying youth from Toronto participated in both the program and research study. Research methods included interviews, vlogs, participant-created media, observational footage, and researcher notes. Analysis involved immersing myself in each participant’s data to holistically consider the creative, technical, and social dimensions of her video-storytelling; I also coded interviews and vlogs to identify themes that united the participants. Inspired by Lange’s (2014) exploration of youth technical identities and Lawrence-Lightfoot’s (1983) narrative portraiture methodology, I crafted a video-storytelling “persona” for each participant, weaving in her own words and media project images. Next, I note the broader significance of relationships and connection as well as video storytelling-specific peer and mentor support for participants. I then discuss their video-making in relation to postfeminist-influenced and video-based social media ecologies and girls’ informal, self-directed media education.</p>
<p>This research honours participants’ stories and critically reflects upon the wide-ranging nature of their video-storytelling experiences and approaches. It also offers initial recommendations for girl-centered programs that emphasize community, support skills development, and provide safer spaces for their media-making and learning. I advocate for girl-specific media-making communities of practice—particularly for marginalized girls—as necessary interventions in evolving media industries and culture to more fully include, support, reflect, and represent diverse populations of girls and women and their stories. </p>
HAK PENDIDIKAN ANAK KORBAN PERANG IRAK TAHUN 2003 BERDASARKAN KONVENSI TENTANG HAK ANAK
Authors: Maulana Rifqi Arif 
Published: May 2023

Children as one of the vulnerable groups who became victims of the war in the armed conflicts of the United States and Iraq in 2003 still have the right to education that should not be ignored. The international community has recognized the right to education as an international right where this arrangement has been stated in the convention on the rights of the child which has been agreed by various countries around the world. This writing aims to analyze the practice of protecting children's education rights based on the convention on children's rights using normative juridical methods so that it has a conclusion that the United States has a big responsibility to restore children's education rights that have been temporarily lost after the conflict is over with various infrastructures damaged in severe levels.
Keywords: children's education rights: conventions on children's rights; United States of America.
PERLINDUNGAN HUKUM TERHADAP FINANCIAL TECHNOLOGY REWARD BASED CROWDFUNDING PADA PROYEK DARI INDUSTRI KREATIF (Studi pada Platfrom Kickstarter.com)
Authors: Hapsari Recca Ayu , Nisa Apri , Putri Agatha jessica 
Published: May 2023

Bank Indonesia stated that Financial Technology/Fintech is the result of a combination of technology and financial services. Platforms such as Kickstarter, Indiegogo, Lending Club, Rock et Hub are becoming increasingly popular for crowdfunding new products and services. The type of contribution that supporters make determines the difference between these platforms. Kickstarter supporters can see first hand the creative process and contribute to the project's success.Several community groups have also developed insights to make the most of internet facilities. The research method used a normative legal approach and an empirical approach. The data collection technique used in this research is library research. Legal protection for financial technology reward based crowdfunding in creative industry projects (Studies on the Kickstarter.com Platform) explains that Kickstarter has adopted a general policy regarding copyright in accordance with the Digital Millennium Copyright Act of 1998.
Keywords: Financial Technology; Technology; Kickstarter.
KAJIAN YURIDIS KERUGIAN KEUNGAN NEGARA AKIBAT KORUPSI DAN KORELASINYA DENGAN HAK WARGA NEGARA
Authors: Yawang Anderson Umbu , Nurdin Fransiskus Saverius , Ndakularak Ernalinda 
Published: May 2023

The practice of criminal acts of corruption in Indonesia is getting more and more apprehensive day by day. This thesis is supported by data 2019 to 2021 released by ICW that corruption practices have increased significantly, causing state financial losses reaching up to IDR 51.928 trillion. The problem of this study is how the correlation of state financial losses due to the practice of criminal acts of corruption by corruptors with the fulfillment of citizens' rights as constitusional rigts but not human rights. This research is a doctrinal legal research with a statutory and conceptual approach. This study concludes that there is an absolute correlation between state financial losses and the non-fulfillment of citizen rights such as work and a decent living.
Keywords: Corruption; State Financial Losses; the Citizen’s Right.
Exploring the sonodynamic effects of bacteriochlorophyll a
Authors: Jia Lanqi , Wang Longhao , Song Yiqiong , Pang Xin , Zhao Jie 
Published: May 2023

Objective: The purpose of this study was to investigate whether bacteriochlorophyll a (BCA) could be used as a potential diagnostic factor in near-infrared fluorescence (NIRF) imaging and in mediating sonodynamic antitumor effect.Methods: The UV spectrum and fluorescence spectra of bacteriochlorophyll a were measured. The IVIS Lumina imaging system was used to observe the fluorescence imaging of bacteriochlorophyll a. 9,10-Dimethylanthracene (DMA) reagent was used as a singlet oxygen sensor to detect singlet oxygen produced by bacteriochlorophyll a. LLC cells of mouse lung adenocarcinoma were selected as experimental subjects. Flow cytometry was used to detect the optimal uptake time of bacteriochlorophyll a in LLC cells. A laser confocal microscope was used to observe the binding of bacteriochlorophyll a to cells. The cell survival rate of each experimental group was detected by the CCK-8 method to detect the cytotoxicity of bacteriochlorophyll a. The effect of BCA-mediated sonodynamic therapy (SDT) on tumor cells was detected by the calcein acetoxymethyl ester/propidium iodide (CAM/PI) double staining method. 2,7-Dichlorodihydrofluorescein-diacetate (DCFH-DA) was used as the staining agent to evaluate and analyze intracellular reactive oxygen species (ROS) levels by fluorescence microscopy and flow cytometry (FCM). A confocal laser scanning microscope (CLSM) was used to observe the localization in the organelles of bacteriochlorophyll a. The IVIS Lumina imaging system was used to observe the fluorescence imaging of BCA in vitro.Results: Bacteriochlorophyll a-mediated SDT significantly increased cytotoxicity to LLC cells compared to other treatments, such as ultrasound (US) only, bacteriochlorophyll a only, and sham therapy. The CLSM observed bacteriochlorophyll a aggregation around the cell membrane and cytoplasm. FCM analysis and fluorescence microscopy showed that bacteriochlorophyll a-mediated SDT in LLC cells significantly inhibited cell growth and caused an obvious increase in intracellular ROS levels, and its fluorescence imaging function suggests that it can be a potential diagnostic factor.Conclusion: The results showed that bacteriochlorophyll a possesses good sonosensitivity and fluorescence imaging function. It can be effectively internalized in LLC cells, and bacteriochlorophyll a-mediated SDT is associated with ROS generation. This suggests that bacteriochlorophyll a can be used as a new type of sound sensitizer, and the bacteriochlorophyll a-mediated sonodynamic effect may be a potential treatment for lung cancer.
Pathological modeling of glycogen storage disease type III with CRISPR/Cas9 edited human pluripotent stem cells
Authors: Rossiaud Lucille , Fragner Pascal , Barbon Elena , Gardin Antoine , Benabides Manon , Pellier Emilie , Cosette Jérémie , El Kassar Lina , Giraud-Triboult Karine , Nissan Xavier , Ronzitti Giuseppe , Hoch Lucile 
Published: May 2023

Introduction: Glycogen storage disease type III (GSDIII) is a rare genetic disease caused by mutations in the AGL gene encoding the glycogen debranching enzyme (GDE). The deficiency of this enzyme, involved in cytosolic glycogen degradation, leads to pathological glycogen accumulation in liver, skeletal muscles and heart. Although the disease manifests with hypoglycemia and liver metabolism impairment, the progressive myopathy is the major disease burden in adult GSDIII patients, without any curative treatment currently available.Methods: Here, we combined the self-renewal and differentiation capabilities of human induced pluripotent stem cells (hiPSCs) with cutting edge CRISPR/Cas9 gene editing technology to establish a stable AGL knockout cell line and to explore glycogen metabolism in GSDIII.Results: Following skeletal muscle cells differentiation of the edited and control hiPSC lines, our study reports that the insertion of a frameshift mutation in AGL gene results in the loss of GDE expression and persistent glycogen accumulation under glucose starvation conditions. Phenotypically, we demonstrated that the edited skeletal muscle cells faithfully recapitulate the phenotype of differentiated skeletal muscle cells of hiPSCs derived from a GSDIII patient. We also demonstrated that treatment with recombinant AAV vectors expressing the human GDE cleared the accumulated glycogen.Discussion: This study describes the first skeletal muscle cell model of GSDIII derived from hiPSCs and establishes a platform to study the mechanisms that contribute to muscle impairments in GSDIII and to assess the therapeutic potential of pharmacological inducers of glycogen degradation or gene therapy approaches.
Oxygen-saturation-related functional parameter as a biomarker for diabetes mellitus—extraction method and clinical validation
Authors: Zhang Jinze , Luo Zhongzhou , Wang Gengyuan , Huang Yuancong , Fei Keyi , Liu Yushuang , Li Jiaxiong , Yuan Jin , Xiao Peng 
Published: May 2023

Purpose: To develop a computational method for oxygen-saturation-related functional parameter analysis of retinal vessels based on traditional color fundus photography, and to explore their characteristic alterations in type 2 diabetes mellitus (DM).Methods: 50 type 2 DM patients with no-clinically detectable retinopathy (NDR) and 50 healthy subjects were enrolled in the study. An optical density ratio (ODR) extraction algorithm based on the separation of oxygen-sensitive and oxygen-insensitive channels in color fundus photography was proposed. With precise vascular network segmentation and arteriovenous labeling, ODRs were acquired from different vascular subgroups, and the global ODR variability (ODRv) was calculated. Student’s t-test was used to analyze the differences of the functional parameters between groups, and regression analysis and receiver operating characteristic (ROC) curves were used to explore the discrimination efficiency of DM patients from healthy subjects based on these functional parameters.Results: There was no significant difference in the baseline characteristics between the NDR and healthy normal groups. The ODRs of all vascular subgroups except the micro venule were significantly higher (p<0.05, respectively) while ODRv was significantly lower (p<0.001) in NDR group than that in healthy normal group. In the regression analysis, the increased ODRs except micro venule and decreased ODRv were significantly correlated with the incidence of DM, and the C-statistic for discrimination DM with all ODR is 0.777 (95% CI 0.687-0.867, p<0.001).Conclusion: A computational method to extract the retinal vascular oxygen-saturation-related optical density ratios (ODRs) with single color fundus photography was developed, and increased ODRs and decreased ODRv of retinal vessels could be new potential image biomarkers of DM.
Aorto-mitral curtain reconstruction in invasive double-valve endocarditis: mid-term outcomes
Authors: Vobornik Martin , Timbilla Salifu , Gofus Jan , Smolak Petr , Chek James Lago , Pojar Marek , Cermakova Eva , Zacek Pavel , Vojacek Jan 
Published: May 2023

BackgroundInvasive double-valve endocarditis with structural damage (abscess or perforation) of the aorto-mitral curtain is a relatively rare but fatal diagnosis requiring complex surgical reconstruction. This study presents the short-term and mid-term outcomes from a single center.MethodsFrom 2014 to 2021, 20 patients with double-valve endocarditis with structural damage of the aorto-mitral curtain underwent surgical reconstruction (Hemi-Commando procedure n = 16 and Commando procedure n = 4). Data were obtained retrospectively.ResultsIn 13 cases, the procedure was a reoperation. The mean cardiopulmonary bypass time was 239 ± 47 min and the mean cross-clamp time was 186 ± 32 min. Concomitant procedures were tricuspid valve repair in two, coronary revascularization in one, closure of a ventricular septal defect in one and hemiarch (using circulatory arrest) in one patient. Eleven patients (55%) required surgical revision for bleeding. Thirty-day mortality was 30% (6 patients)—3 patients from the Hemi-Commando group (19%) and 3 patients from the Commando group (75%). Overall survival at 1, 3 and 5 years was 60%, 50% and 45% respectively. Reoperation was required by 4 patients. Freedom from reoperation at 1, 3 and 5 years was 86%, 71% and 71% respectively.ConclusionDespite the high postoperative morbidity and mortality, complex surgical reconstruction of the aorto-mitral continuity of patients with double-valve endocarditis represents the only real chance for survival. Mid-term outcomes are acceptable, but strict follow-up is required due to the risk of valve failure.
The NMDA receptor regulates integrin activation, ATP release and arterial thrombosis through store-operated Ca2+ entry in platelets
Authors: Reusswig Friedrich , Yilmaz Münteha , Brechtenkamp Marius , Krueger Irena , Metz Lisa Maria , Klöcker Nikolaj , Lammert Eckhard , Elvers Margitta 
Published: May 2023

IntroductionPlatelet activation and thrombus formation is crucial for hemostasis, but also trigger arterial thrombosis. Calcium mobilization plays an important role in platelet activation, because many cellular processes depend on the level of intracellular Ca2+ ([Ca2+](i)), such as integrin activation, degranulation, cytoskeletal reorganization. Different modulators of Ca2+ signaling have been implied, such as STIM1, Orai1, CyPA, SGK1, etc. Also, the N-methyl-D-aspartate receptor (NMDAR) was identified to contribute to Ca2+ signaling in platelets. However, the role of the NMDAR in thrombus formation is not well defined.MethodsIn vitro and in vivo analysis of platelet-specific NMDAR knock-out mice.ResultsIn this study, we analyzed Grin1fl/fl-Pf4-Cre+ mice with a platelet-specific knock-out of the essential GluN1 subunit of the NMDAR. We found reduced store-operated Ca2+ entry (SOCE), but unaltered store release in GluN1-deficient platelets. Defective SOCE resulted in reduced Src and PKC substrate phosphorylation following stimulation of glycoprotein (GP)VI or the thrombin receptor PAR4 followed by decreased integrin activation but unaltered degranulation. Consequently, thrombus formation on collagen under flow conditions was reduced ex vivo, and Grin1fl/fl-Pf4-Cre+ mice were protected against arterial thrombosis. Results from human platelets treated with the NMDAR antagonist MK-801 revealed a crucial role of the NMDAR in integrin activation and Ca2+ homeostasis in human platelets as well.ConclusionNMDAR signaling is important for SOCE in platelets and contributes to platelet activation and arterial thrombosis. Thus, the NMDAR represents a novel target for anti-platelet therapy in cardiovascular disease (CVD).
Two modes of gypsum replacement by carbonate and native sulfur in the Lorca Basin, SE Spain
Authors: Rouwendaal Simon E. , Birgel Daniel , Grossi Vincent , Aloisi Giovanni , Guibourdenche Laetitia , Labrado Amanda L. , Brunner Benjamin , Rouchy Jean-Marie , Peckmann Jörn 
Published: May 2023

Organoclastic sulfate reduction and bacterial sulfide oxidation have been suggested to explain the formation of authigenic carbonate and native sulfur replacing gypsum in the Lorca Basin, Spain. To gain more insight into the nature of this replacement, two types of sulfur-bearing carbonate (laminated and brecciated) from the late Miocene Lorca Basin were studied. Petrographic observations revealed that a sulfur-bearing laminated carbonate consists of clay-rich and dolomite-rich laminae with carbonate and native sulfur pseudomorphs after gypsum. Positive δ18Ocarbonate values in the laminae (δ18O = 2.6‰) and lipid biomarkers of halophilic archaea (e.g., extended archaeol) suggest formation under hypersaline conditions. Bacterial sulfate reduction, evidenced by biomarkers such as iso-C15, iso-C16, and iso-C17 fatty acids, produced hydrogen sulfide inducing the abiotic formation of organic sulfur compounds. Gypsum in the laminated carbonate likely dissolved due to undersaturation as evidenced by a low content of carbonate-associated sulfate (3,668 ppm) and 34S-enriched native sulfur (δ34S = 22.4‰), reflecting sulfate limitation. Such 34S-enrichment implies limited fluid flow, which probably restricted the supply of molecular oxygen required for native sulfur formation through oxidation of hydrogen sulfide. Alternatively, sulfate-reducing bacteria may have mediated native sulfur formation directly as a stress response to environmental conditions. The formation of sulfur-bearing calcite in brecciated carbonates is due to post-depositional alteration. Negative δ18O values of the calcite (δ18O = −1.5‰) and a tenfold decrease in carbonate-associated sulfate content (752 ppm) suggest gypsum dissolution and subsequent calcite precipitation from meteoric water. Relatively 34S-depleted native sulfur (δ34S = 13.1‰) leaves it ambiguous whether meteoric water influx could have supplied sufficient molecular oxygen for oxidation of hydrogen sulfide. In case of the brecciated carbonate, methanogenesis, anaerobic oxidation of methane, and bacterial sulfate reduction apparently mediated the formation of secondary minerals as indicated by 13C-depleted lipid biomarkers representative for the respective metabolisms. This study reveals that the conditions and timing of gypsum replacement are variable–taking place 1) during or shortly after gypsum deposition or 2) significantly after sedimentation–and suggests that methanogens in addition to anaerobic methanotrophic archaea and sulfate-reducing bacteria may be involved in the mineral-forming processes in the sedimentary subsurface.
“To brain or not to brain”: evaluating the possible direct effects of the satiety factor oleoylethanolamide in the central nervous system
Authors: Romano Adele , Friuli Marzia , Eramo Barbara , Gallelli Cristina Anna , Koczwara Justyna Barbara , Azari Elnaz Karimian , Paquot Adrien , Arnold Myrtha , Langhans Wolfgang , Muccioli Giulio G. , Lutz Thomas Alexander , Gaetani Silvana 
Published: May 2023

IntroductionOleoylethanolamide (OEA), an endogenous N-acylethanolamine acting as a gut-to-brain signal to control food intake and metabolism, has been attracting attention as a target for novel therapies against obesity and eating disorders. Numerous observations suggested that the OEA effects might be peripherally mediated, although they involve central pathways including noradrenergic, histaminergic and oxytocinergic systems of the brainstem and the hypothalamus. Whether these pathways are activated directly by OEA or whether they are downstream of afferent nerves is still highly debated. Some early studies suggested vagal afferent fibers as the main route, but our previous observations have contradicted this idea and led us to consider the blood circulation as an alternative way for OEA’s central actions.MethodsTo test this hypothesis, we first investigated the impact of subdiaphragmatic vagal deafferentation (SDA) on the OEA-induced activation of selected brain nuclei. Then, we analyzed the pattern of OEA distribution in plasma and brain at different time points after intraperitoneal administration in addition to measuring food intake.ResultsConfirming and extending our previous findings that subdiaphragmatic vagal afferents are not necessary for the eating-inhibitory effect of exogenous OEA, our present results demonstrate that vagal sensory fibers are also not necessary for the neurochemical effects of OEA. Rather, within a few minutes after intraperitoneal administration, we found an increased concentration of intact OEA in different brain areas, associated with the inhibition of food intake.ConclusionOur results support that systemic OEA rapidly reaches the brain via the circulation and inhibits eating by acting directly on selected brain nuclei.
Distribution of gold derived from hydrothermal fluids on the modern seafloor and its impact on the gold budget of seawater
Authors: Huang Wei , Liang Jin , Lu Jingfang , Hou Fanghui , Li Panfeng , Cui Ruyong 
Published: May 2023

The modern seafloor hydrothermal system plays a significant role in the cycling of energy and mass between the internal and external layers of the oceanic crust and upper mantle. It continues to supply hydrothermal fluids containing three to five orders of magnitude more gold into the ocean than the amount typically present in deep seawater. It has a considerable impact on the distribution and budget of gold with respect to the large geological inventory of the ocean along with other input sources such as river water. The large amount of various types of data compiled for this study reveals that only about 0.3% of the annual hydrothermal flux of gold (2618.3 kg/a) injected into the overlying seawater column as a dissolved phase is eventually trapped in sulfide deposits near vent sites on the seafloor, while about 0.8% is trapped in metalliferous sediments that fall out from the distal nonbuoyant plume. The remaining ~98.9% of gold is delivered into the depths of the global open ocean. The global budget of gold in seawater (about 1.4 × 107 kg), the annual flux of hydrothermal fluids at the seafloor (about 2.6 × 103 kg/a), the amount delivered by river water (about 7.2 × 104 kg/a), and significant estuarine removal (15%) allows us to estimate the residence time of gold in the modern ocean to be about 220 years. This value is 70% shorter than that (~1000 years) reported previously. In the future, the use of appropriate artificial means to achieve more efficient precipitation of gold from the hydrothermal system at the seafloor could increase the level of enrichment of gold to obtain gold-rich hydrothermal deposits, yielding greater economic benefits.