bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climate, Women, and Conflict: Rebel Groups’ Armed Activities after Major Disasters
Authors: Ide Tobias 
Published: August 2023

Abstract
This study builds long-anticipated bridges between the literature on (i) climate/disaster security, (ii) women, peace, and security, and (iii) rebel group behavior. Specifically, I analyze how rebel groups react to disasters (like droughts, earthquakes, floods, or storms) by intensifying or reducing their armed activities. This addresses a crucial knowledge gap: While a large literature is dealing with the impacts of climate change and disasters on armed conflict onset or incidence, knowledge on how environmental factors shape the behavior of particular conflict actors remains scarce. I argue that rebel groups escalate fighting when disaster-related opportunities occur (e.g., easier recruitment) but scale back their armed activities if adversely affected by the disasters (e.g., loss of resources). However, this relationship is contingent of the rebels’ inclusion of female combatants. Empirically, this study draws on a unique sample of thirty-six cases of disasters striking armed conflict zones and integrates quantitative and qualitative data via a qualitative comparative analysis. The results suggest that rebel groups intensify their armed activities to exploit disaster-related opportunities but can only do so when female combatants provide them with sufficient capabilities. Conversely, rebels have to reduce fighting when adversely affected by disasters unless they can draw on female members to cope with disaster impacts.
The cross-reactivity of cannabinoid analogs (delta-8-THC, delta-10-THC and CBD), their metabolites and chiral carboxy HHC metabolites in urine of six commercially available homogeneous immunoassays
Authors: Wolf Carl E , Pokhai Ashley A , Poklis Justin L , Williams Grace R 
Published: August 2023

Abstract
There has been an exponential surge in the presence and use of cannabinoids since the federal legalization of hemp (Agricultural Improvement Act of 2018). This growth is attributed to delta-9-tetrahydrocannabinol (delta-9-THC) and cannabidiol (CBD), the most abundant phytocannabinoid components of cannabis and hemp, respectively, but with many other emerging THC analogs. Structurally, these analogs are similar to delta-9-THC, yet very little information is available about their potency and even less information is available regarding their detectability using commercially available cannabinoid screening kits. Due to their structural similarity, current cannabinoid homogeneous immunoassay screening methods may be able to detect these emerging cannabinoid analogs and their metabolites. Six urine immunoassay kits (Abbott Cannabinoids—Abbott Diagnostics, LZI Cannabinoids (cTHC) Enzyme Immunoassay—Lin-Zhi International, DRI® Cannabinoid Assay and CEDIA™ THC—Thermo Fisher Scientific, ONLINE DAT Cannabinoid II—Roche Diagnostics and Syva EMIT®II Plus—Siemens Healthineers) were evaluated at two different cutoff concentrations: 50 ng/mL and 20 or 25 ng/mL, assay dependent. The analysis was performed on an Abbott Architect Plus c4000 (Abbott Diagnostics). Delta-8-THC, CBD, olivetol and their major metabolites, and delta-10-THC and HHC carboxylic acid chiral analogs were evaluated. The cross-reactivity was evaluated by preparing each analyte at 20, 50, 100 and 1,000 ng/mL in urine. Analytes that did not cross-react at 1,000 ng/mL for a cutoff were considered not detectable. If detected, the lowest concentration was used as the decision point to determine the precision at the immunoassay’s cutoff. The six commercially available urine cannabinoid homogeneous immunoassay screening kits cross reacted with the following analogs: delta-8-THC, 11-OH-delta-8-THC, 11-COOH-delta-8-THC, 6-OH-CBD, 7-OH-CBD, all delta-10-THC and HHC carboxylic acid chiral analogs and olivetol with varying selectivity depending on the screening kit and cutoff concentration. The kits did not cross-react with the following analogs: CBD, 7-COOH-CBD, Abnormal CBD, CBDA-A and olivetolic acid.
Null models to explain highly diverse community of intertidal decapods on a temperate rocky coast in Antofagasta, northern Chile (23º27ʹS)
Authors: De los Ríos-Escalante Patricio , Ibáñez-Arancibia Eliana , Esse Carlos , Zúñiga Oscar , Fajardo Maritza , Ghory Farhana S 
Published: August 2023

AbstractThe decapod fauna on intertidal shores in northern Chile is characterized by its relatively high diversity and abundance due to the presence of continuous upwelling by the Humboldt Current, which results in a high productivity. The decapod fauna on intertidal rocky shores is characterized by coexisting species that inhabits in natural shelters such as cracks and eroded rocks, as well as among macroalgae. We aimed to monitor the brachyuran and anomuran (porcellanid) crabs in three intertidal sites during two periods of time in September-December 2019 in the Antofagasta region, one site without human intervention and two sites in the city of Antofagasta, for which we applied null models in ecology. We assumed that the communities are random, a viewpoint that is more robust because it reduces the risk of error type I. The results of the application of null models on the co-occurrence species revealed that species associations are mainly structured for all sites, whereas the niche-sharing null model revealed that the reported species did not share ecological niches and in consequence there is not interspecific competition. The results would probably indicate that the species of intertidal decapods have a micro-specific ecological-niche differentiation, which would explain the existence of structured pattern in species associations. The results were compared with information for other sites along the continental Chilean coast.
Roadblocks to Disarmament in the Nuclear Non-Proliferation Treaty System
Authors: Hood Anna 
Published: August 2023

Abstract
For the last five decades, the states parties to the Nuclear Non-Proliferation Treaty (NPT) have come together every 5 years at Review Conferences to explore how they can advance the Treaty’s goals, including the pursuit of nuclear disarmament. Despite lengthy negotiations, the Review Conferences have failed to reach a consensus final document as many times as they have succeeded and even when a final document has been agreed it has contained few tangible gains for those working towards a nuclear weapon free world. This state of affairs played out again in 2022 when the NPT’s 10th Review Conference ended without agreement and with many states decrying the weakness of the disarmament provisions that had been under discussion. It has been common in the aftermath of Review Conferences for academics to consider the contemporary political issues that stymied progress. While such studies are important, it is also necessary to consider what deeper structural issues may be at play in preventing the goal of nuclear disarmament from being advanced in the NPT context. To this end, this article identifies and analyses three key structural issues with the NPT and NPT Review Conferences that create difficulties for those pursuing a nuclear disarmament agenda. First, it explores issues with the scope and content of Article VI and how it has been interpreted by the nuclear weapon states. Secondly, it looks at the difficulties that arise from the commitment to achieving consensus outcomes at the Review Conferences and, thirdly, it considers the limitations of who actually attends and contributes to Review Conferences.
Recent trends in radiotherapy use for major cancers in Korea
Authors: Yang Kyungmi , Lee Jeong Eun , Park Won , Ahn Yong Chan , Huh Seung Jae 
Published: August 2023

Abstract

Background
Although the trend in radiotherapy in all cancer patients has been studied, changes in radiotherapy modalities for specific cancer types have not been reported. This study aimed to analyze radiotherapy patterns for major cancers in Korea in recent years.

Materials and Methods
We collected data from claims and reimbursement records of the Health and Insurance Review and Assessment Service from 2017 to 2020, according to initial diagnostic codes. The radiotherapy modalities for major cancers, such as lung, stomach, colorectal, breast and liver cancer, were analyzed. The radiotherapy modalities consisted of two-dimensional radiotherapy, three-dimensional conformal radiotherapy, intensity-modulated radiotherapy, proton radiotherapy and stereotactic body radiotherapy.

Results
Overall, from 2017 to 2020, the use of two-dimensional radiotherapy and three-dimensional conformal radiotherapy decreased, and intensity-modulated radiotherapy increased. In 2017, three-dimensional conformal radiotherapy accounted for approximately half of the radiotherapy in patients for lung and colorectal cancer, which was replaced by intensity-modulated radiotherapy in 2020. In 2020, stereotactic body radiotherapy also accounted for a large proportion of radiotherapy used in liver cancer cases. Intensity-modulated radiotherapy was most used, followed by three-dimensional conformal radiotherapy and two-dimensional radiotherapy for breast cancer in 2020. Among major cancers, radiotherapy utilization for breast cancer is the highest. Compared with other cancers, the number of patients receiving radiotherapy for stomach cancer was low.

Conclusion
The number of patients receiving radiotherapy for major cancers has increased. The use of advanced forms of radiotherapy, such as intensity-modulated radiotherapy, is rapidly increasing for major cancers. The rate of radiotherapy utilization was higher in major cancer patients than in all cancer patients.
Laparoscopic minor liver resection for hepatocellular carcinoma
Authors: Kokudo Takashi , Takemura Nobuyuki , Inagaki Fuyuki , Yoshizaki Yuhi , Mihara Fuminori , Edamoto Yoshihiro , Yamada Kazuhiko , Kokudo Norihiro 
Published: August 2023

Abstract

Background
Previous studies have suggested that laparoscopic liver resection for hepatocellular carcinoma is associated with lower postoperative complications compared with open liver resection.

Methods
We conducted a retrospective analysis of 109 hepatocellular carcinoma patients who underwent minor liver resection at a Japanese tertiary care hospital from November 2010 to December 2022.

Results
The laparoscopic liver resection group experienced significantly lower median intraoperative blood loss compared with the open liver resection group (P = 0.0001). Furthermore, the laparoscopic liver resection group had a significantly shorter median hospital stay compared with the open liver resection group (P = 0.0002). However, there was no significant difference in median postoperative survival between the laparoscopic liver resection group and the open liver resection group (P = 0.717).

Conclusions
Laparoscopic minor liver resection for hepatocellular carcinoma resulted in reduced blood loss and hospital stay without compromising long-term survival outcomes.
Algorithmic Harm in Consumer Markets
Authors: Bar-Gill Oren , Sunstein Cass R , Talgam-Cohen Inbal 
Published: August 2023

Abstract
Machine learning algorithms are increasingly able to predict what goods and services particular people will buy, and at what price. It is possible to imagine a situation in which relatively uniform, or coarsely set, prices and product characteristics are replaced by far more in the way of individualization. Companies might, for example, offer people shirts and shoes that are particularly suited to their situations, that fit with their particular tastes, and that have prices that fit their personal valuations. In many cases, the use of algorithms promises to increase efficiency and to promote social welfare; it might also promote fair distribution. But when consumers suffer from an absence of information or from behavioral biases, algorithms can cause serious harm. Companies might, for example, exploit such biases in order to lead people to purchase products that have little or no value for them or to pay too much for products that do have value for them. Algorithmic harm, understood as the exploitation of an absence of information or of behavioral biases, can disproportionately affect members of identifiable groups, including women and people of color. Since algorithms exacerbate the harm caused to imperfectly informed and imperfectly rational consumers, their increasing use provides fresh support for existing efforts to reduce information and rationality deficits, especially through optimally designed disclosure mandates. In addition, there is a more particular need for algorithm-centered policy responses. Specifically, algorithmic transparency—transparency about the nature, uses, and consequences of algorithms—is both crucial and challenging; novel methods designed to open the algorithmic “black box” and “interpret” the algorithm’s decision-making process should play a key role. In appropriate cases, regulators should also police the design and implementation of algorithms, with a particular emphasis on the exploitation of an absence of information or of behavioral biases.
Do economic incentives promote physical activity? Evidence from the London Congestion Charge
Authors: Nakamura Ryota , Albanese Andrea , Coombes Emma , Suhrcke Marc 
Published: August 2023

Abstract
This study investigates the impact of economic incentives on travel-related physical activity, leveraging the London Congestion Charge’s disincentivising of sedentary travel modes via increasing the cost of private car use within Central London. The scheme imposes charges on most types of cars entering, exiting, and operating within the Central London area, while individuals living inside the charging zone are eligible for a 90% reduction in congestion charges. Geographical location information provides the full-digit postcode data necessary to precisely identify the eligibility for the discount of participants in the London Travel Demand Survey for the period 2005–2011. Using a boundary regression-discontinuity design reveals a statistically significantly positive impact on active commuting (i.e. cycling and walking) around the border of the charging zone. The effect is larger for lower-income households and car owners. The findings are robust against multiple specifications and validation tests.
Bearded or smooth? Awns improve yield when wheat experiences heat stress during grain fill in the southeastern United States
Authors: DeWitt Noah , Lyerly Jeanette , Guedira Mohammed , Holland James B , Murphy J Paul , Ward Brian P , Boyles Richard E , Mergoum Mohamed , Babar Md Ali , Shakiba Ehsan , Sutton Russel , Ibrahim Amir , Tiwari Vijay , Santantonio Nicholas , Van Sanford David A , Howell Kimberly , Smith Jared H , Harrison Stephen A , Brown-Guedira Gina 
Published: August 2023

Abstract
The presence or absence of awns—whether wheat heads are ‘bearded’ or ‘smooth’ – is the most visible phenotype distinguishing wheat cultivars. Previous studies suggest that awns may improve yields in heat or water-stressed environments, but the exact contribution of awns to yield differences remains unclear. Here we leverage historical phenotypic, genotypic, and climate data for wheat (Triticum aestivum) to estimate the yield effects of awns under different environmental conditions over a 12-year period in the southeastern USA. Lines were classified as awned or awnless based on sequence data, and observed heading dates were used to associate grain fill periods of each line in each environment with climatic data and grain yield. In most environments, awn suppression was associated with higher yields, but awns were associated with better performance in heat-stressed environments more common at southern locations. Wheat breeders in environments where awns are only beneficial in some years may consider selection for awned lines to reduce year-to-year yield variability, and with an eye towards future climates.
Hierarchical contribution of Argonaute proteins to antiviral protection
Authors: Ludman Márta , Szalai Gabriella , Janda Tibor , Fátyol Károly 
Published: August 2023

Abstract
Antiviral RNAi is the main protective measure employed by plants in the fight against viruses. The main steps of this process have been clarified in recent years, primarily relying on the extensive genetic resources of Arabidopsis thaliana. Our knowledge of viral diseases of crops, however, is still limited, mainly due to the fact that A. thaliana is a non-host for many agriculturally important viruses. In contrast, Nicotiana benthamiana has an unparalleled susceptibility to viruses and, since it belongs to the Solanaceae family, it is considered an adequate system for modeling infectious diseases of crops such as tomatoes. We used a series of N. benthamiana mutants created by genome editing to analyze the RNAi response elicited by the emerging tomato pathogen, pepino mosaic virus (PepMV). We uncovered hierarchical roles of several Argonaute proteins (AGOs) in anti-PepMV defense, with the predominant contribution of AGO2. Interestingly, the anti-PepMV activities of AGO1A, AGO5, and AGO10 only become apparent when AGO2 is mutated. Taken together, our results prove that hierarchical actions of several AGOs are needed for the plant to build effective anti-PepMV resistance. The genetic resources created here will be valuable assets for analyzing RNAi responses triggered by other agriculturally important pathogenic viruses.
TeloBase: a community-curated database of telomere sequences across the tree of life
Authors: Lyčka Martin , Bubeník Michal , Závodník Michal , Peska Vratislav , Fajkus Petr , Demko Martin , Fajkus Jiří , Fojtová Miloslava 
Published: August 2023

Abstract
Discoveries over the recent decade have demonstrated the unexpected diversity of telomere DNA motifs in nature. However, currently available resources, ‘Telomerase database’ and ‘Plant rDNA database’, contain just fragments of all relevant literature published over decades of telomere research as they have a different primary focus and limited updates. To fill this gap, we gathered data about telomere DNA sequences from a thorough literature screen as well as by analysing publicly available NGS data, and we created TeloBase (http://cfb.ceitec.muni.cz/telobase/) as a comprehensive database of information about telomere motif diversity. TeloBase is supplemented by internal taxonomy utilizing popular on-line taxonomic resources that enables in-house data filtration and graphical visualisation of telomere DNA evolutionary dynamics in the form of heat tree plots. TeloBase avoids overreliance on administrators for future data updates by having a simple form and community-curation system for application and approval, respectively, of new telomere sequences by users, which should ensure timeliness of the database and topicality. To demonstrate TeloBase utility, we examined telomere motif diversity in species from the fungal genus Aspergillus, and discovered (TTTATTAGGG)n sequence as a putative telomere motif in the plant family Chrysobalanaceae. This was bioinformatically confirmed by analysing template regions of identified telomerase RNAs.
spotter: a single-nucleotide resolution stochastic simulation model of supercoiling-mediated transcription and translation in prokaryotes
Authors: Hacker William C , Elcock Adrian H 
Published: August 2023

Abstract
Stochastic simulation models have played an important role in efforts to understand the mechanistic basis of prokaryotic transcription and translation. Despite the fundamental linkage of these processes in bacterial cells, however, most simulation models have been limited to representations of either transcription or translation. In addition, the available simulation models typically either attempt to recapitulate data from single-molecule experiments without considering cellular-scale high-throughput sequencing data or, conversely, seek to reproduce cellular-scale data without paying close attention to many of the mechanistic details. To address these limitations, we here present spotter (Simulation of Prokaryotic Operon Transcription & Translation Elongation Reactions), a flexible, user-friendly simulation model that offers highly-detailed combined representations of prokaryotic transcription, translation, and DNA supercoiling. In incorporating nascent transcript and ribosomal profiling sequencing data, spotter provides a critical bridge between data collected in single-molecule experiments and data collected at the cellular scale. Importantly, in addition to rapidly generating output that can be aggregated for comparison with next-generation sequencing and proteomics data, spotter produces residue-level positional information that can be used to visualize individual simulation trajectories in detail. We anticipate that spotter will be a useful tool in exploring the interplay of processes that are crucially linked in prokaryotes.
Increased levels of eIF2A inhibit translation by sequestering 40S ribosomal subunits
Authors: Grove Daisy J , Levine Daniel J , Kearse Michael G 
Published: August 2023

Abstract
eIF2A was the first eukaryotic initiator tRNA carrier discovered but its exact function has remained enigmatic. Uncharacteristic of translation initiation factors, eIF2A is reported to be non-cytosolic in multiple human cancer cell lines. Attempts to study eIF2A mechanistically have been limited by the inability to achieve high yield of soluble recombinant protein. Here, we developed a purification paradigm that yields ∼360-fold and ∼6000-fold more recombinant human eIF2A from Escherichia coli and insect cells, respectively, than previous reports. Using a mammalian in vitro translation system, we found that increased levels of recombinant human eIF2A inhibit translation of multiple reporter mRNAs, including those that are translated by cognate and near-cognate start codons, and does so prior to start codon recognition. eIF2A also inhibited translation directed by all four types of cap-independent viral IRESs, including the CrPV IGR IRES that does not require initiation factors or initiator tRNA, suggesting excess eIF2A sequesters 40S subunits. Supplementation with additional 40S subunits prevented eIF2A-mediated inhibition and pull-down assays demonstrated direct binding between recombinant eIF2A and purified 40S subunits. These data support a model that eIF2A must be kept away from the translation machinery to avoid sequestering 40S ribosomal subunits.
A primer-independent DNA polymerase-based method for competent whole-genome amplification of intermediate to high GC sequences
Authors: Ordóñez Carlos D , Mayoral-Campos Carmen , Egas Conceição , Redrejo-Rodríguez Modesto 
Published: August 2023

Abstract
Multiple displacement amplification (MDA) has proven to be a useful technique for obtaining large amounts of DNA from tiny samples in genomics and metagenomics. However, MDA has limitations, such as amplification artifacts and biases that can interfere with subsequent quantitative analysis. To overcome these challenges, alternative methods and engineered DNA polymerase variants have been developed. Here, we present new MDA protocols based on the primer-independent DNA polymerase (piPolB), a replicative-like DNA polymerase endowed with DNA priming and proofreading capacities. These new methods were tested on a genomes mixture containing diverse sequences with high-GC content, followed by deep sequencing. Protocols relying on piPolB as a single enzyme cannot achieve competent amplification due to its limited processivity and the presence of ab initio DNA synthesis. However, an alternative method called piMDA, which combines piPolB with Φ29 DNA polymerase, allows proficient and faithful amplification of the genomes. In addition, the prior denaturation step commonly performed in MDA protocols is dispensable, resulting in a more straightforward protocol. In summary, piMDA outperforms commercial methods in the amplification of genomes and metagenomes containing high GC sequences and exhibits similar profiling, error rate and variant determination as the non-amplified samples.