bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of shear force on ex vivo expansion of hematopoietic model cells in a stirred tank bioreactor
Authors: Mohseni Sofia , Khoshfetrat Ali Baradar , Rahbarghazi Reza , Khodabakhshaghdam Shahla , Kaleybar Leila Shafiei 
Published: June 2023

AbstractTo evaluate shear stress influence on ex vivo expansion of hematopoietic cell lineages for clinical application, in this study, human pro-monocytic cell (namely U937 cell line) was selected as a hematopoietic stem cell (HSC) model and cultured in suspension mode at two different agitation rates (50, 100 rpm) in the stirred bioreactor. At the agitation rate of 50 rpm, the cells achieved higher expansion folds (27.4 fold) with minimal morphological changes as well as apoptotic cell death, while at 100 rpm the expansion fold decreased after 5-day of culture in suspension culture in comparison with static culture and reached 24.5 fold at the end of the culture. The results of glucose consumption and lactate production were also in agreement with the data of fold expansion and indicated the preference of culture in the stirred bioreactor when agitated at 50 rpm. This study indicated the stirred bioreactor system with an agitation rate of 50 rpm and surface aeration may be used as a potential dynamic culture system for clinical applications of hematopoietic cell lineage. The current experiments shed data related to the effect of shear stress on human U937 cells, as a hematopoietic cell model, to set a protocol for expansion of HSCs for biomedical applications.
Panniculectomy as a surgical option for the management of a deep surgical site infection after C-section in a morbidly obese woman: a case report
Authors: Kamla Joël Igor , Bwelle Georges Motto , Tochie Joel Noutakdie , Tchuenkam Landry Wakheu , Wandji Brigitte , Kamto Trevor , Esiéné Agnès 
Published: June 2023

Abstract
Background
Obesity is an independent risk factor for the occurrence of surgical site infections (SSIs) following all types of surgeries, especially after Caesarean section (C-section). SSIs increase postoperative morbidity, health economic cost and their management is quiet complex with no universal therapeutic consensus. Herein, we report a challenging case of a deep SSI after C-section in a central morbidly obese woman managed successfully by panniculectomy.

Case presentation
A 30-year-old black African pregnant woman with marked abdominal panniculus extending to the pubic area, a waist circumference = 162 cm and BMI = 47.7 kg/m2 underwent an emergency CS indicated for acute fetal distress. By day five post-operation, she developed a deep parietal incisional infection unremitting to antibiotic therapy, wound dressings and beside wound debridement till the 26th postoperative day. A large abdomen panniculus and maceration of the wound enhanced by central obesity increased the risk of failure of spontaneous closure; thus, an abdominoplasty by panniculectomy was indicated. The patient underwent panniculectomy on the 26th day after the initial surgery and her post-operative course was uneventful. Wound esthetics was satisfactory three months later. Adjuvant dietary and psychological management were associated.

Conclusion
Post-Caesarean deep SSI is a frequent complication in obese patients. A panniculectomy may be a safe and promising therapeutic surgical option with good cosmetic results and little postoperative complications when used in a multidisciplinary anti-obesogenic approach.
Pollination success of Fraxinus excelsior L. in the context of ash dieback
Authors: Eisen Anna-Katharina , Semizer-Cuming Devrim , Jochner-Oette Susanne , Fussi Barbara 
Published: June 2023

Abstract

Key message
Paternity analyses show that effective pollination of ash (Fraxinus excelsior L.) in a seed orchard and a floodplain forest affected by ash dieback is more likely to be facilitated by healthier males. Thereby, natural selection can have a positive effect on the health of future generations.

Context
Ongoing ash dieback and increasing fragmentation of ash populations may result in reduced pollen flow, which can reduce pollination success of future generations of ash trees. Therefore, it is essential to further improve our understanding of gene flow patterns, especially with respect to ash dieback.

Aims
In this study, paternity analyses were conducted in a seed orchard and a floodplain forest in Germany in 2018 to explain the relationship between pollination success and the health status of ash trees and distances of effective pollen transport.

Methods
Cambium samples (i.e., from twigs and stumps) were collected from 251 ash trees (putative father and mother trees) for genotyping, and the health status of each tree was documented using a scoring system to evaluate vitality. Additionally, seeds were harvested from 12 mother trees per site. Genetic analyses using nuclear microsatellites were performed to determine paternal trees. Paternities were assigned based on the likelihood model implemented in the Cervus 3.0.7 software.

Results
Our results showed that the average pollination distance was 76 m in the seed orchard and 166 m in the floodplain forest. In general, pollination success decreased substantially with increasing distance to the mother tree. Despite the dense tree cover in the floodplain forest, pollen were transported over long distances (greater than 550 m), suggesting that non-local sources also play a role in pollination. This is supported by the foreign pollen input identified in the seed orchard (66.5%). Self-pollination was detected only to a very small extent, and thus had no major influence on reproduction. In addition, both healthy and slightly diseased father trees showed similar mating success. However, this was not the case for the severely diseased ash trees (more than 50% of crown damage) because only a few offspring could be assigned to them. Nevertheless, in contrast to the floodplain forest, there was no significant correlation between damage classes and pollination success in the seed orchard.

Conclusion
Long-distance pollen transport contributes to the connectivity of ash trees in the landscape. Additionally, both healthy and slightly diseased fathers have a greater contribution to pollination, thus potentially improving the health of the next generation of ash trees. Moreover, gene flow between stepping stone populations is necessary to ensure the positive impact on the genetic diversity of ash populations in the future.
PEGylated magnetic nanographene oxide for targeted delivery of arsenic trioxide and sec-o-glucosylhamaudol in tumor treatment with improved dual-drugs synergistic effect
Authors: Cheng Jinlai , Hong Kun , Sun Jianhui , Li Hongmei , Zhao Yu , Zhao Qinghe , Tan Yuqing , Yang Miyi 
Published: June 2023

AbstractArsenic trioxide (ATO) is a promising chemotherapeutic agent, but its clinical application is limited due to its poor pharmacokinetics and dose-limited toxicity. Moreover, the combination of ATO and sec-o-glucosylhamaudol (SOG) can improve the therapeutic effect of hepatoma. In this study, PEGylated magnetic nanographene oxide (PEG@MGO) was used as magnetic carriers to enhance the targeting ability of the drug delivery system. ATO and SOG are loaded on the surface of PEG@MGO nanoparticles through electrostatic interactions. This biocompatible nanocomposite shows magnetic susceptibility, pH sensitivity, and high loading capacity of the drugs. The in vitro cytotoxicity study of human hepatoma cell line (HepG2) cells showed more significant cytotoxicity and obvious synergistic effect between ATO and SOG compared with that of single drug-loaded nanoparticles via MTT assay. In vitro cellular uptake was observed by Prussian blue staining and fluorescently labeling. The results demonstrated a high cellular internalization rate of PEG@MGO. The ATO and SOG co-loaded nanodrug significantly inhibits the growth of tumors in vivo, which might be due to the oxidative stress and proapoptotic effect. This type of multidrug nanocomposite offers a promising alternative for cancer therapy.
Graphical Abstract
A pH-sensitive polyethylene glycol-modified magnetic graphene oxide loaded with ATO and SOG (PEG@MGO@ATO + SOG) was prepared for the magnetically targeted and efficient synergistic-chemo cancer therapy, which exhibited high specificity and good biocompatibility.
Effectiveness of routine provision of feedback from patient‐reported outcome measurements for cancer care improvement: a systematic review and meta-analysis
Authors: Lu Sheng-Chieh , Porter I. , Valderas J. M. , Harrison C. J. , Sidey-Gibbons Chris 
Published: June 2023

Abstract
Background
Research shows that feeding back patient-reported outcome information to clinicians and/or patients could be associated with improved care processes and patient outcomes. Quantitative syntheses of intervention effects on oncology patient outcomes are lacking.

Objective
To determine the effects of patient-reported outcome measure (PROM) feedback intervention on oncology patient outcomes.

Data sources
We identified relevant studies from 116 references included in our previous Cochrane review assessing the intervention for the general population. In May 2022, we conducted a systematic search in five bibliography databases using predefined keywords for additional studies published after the Cochrane review.

Study selection
We included randomized controlled trials evaluating the effects of PROM feedback intervention on processes and outcomes of care for oncology patients.

Data extraction and synthesis
We used the meta-analytic approach to synthesize across studies measuring the same outcomes. We estimated pooled effects of the intervention on outcomes using Cohen’s d for continuous data and risk ratio (RR) with a 95% confidence interval for dichotomous data. We used a descriptive approach to summarize studies which reported insufficient data for a meta-analysis.

Main outcome(s) and measures(s)
Health-related quality of life (HRQL), symptoms, patient-healthcare provider communication, number of visits and hospitalizations, number of adverse events, and overall survival.

Results
We included 29 studies involving 7071 cancer participants. A small number of studies was available for each metanalysis (median = 3 studies, ranging from 2 to 9 studies) due to heterogeneity in the evaluation of the trials. We found that the intervention improved HRQL (Cohen’s d = 0.23, 95% CI 0.11–0.34), mental functioning (Cohen’s d = 0.14, 95% CI 0.02–0.26), patient-healthcare provider communication (Cohen’s d = 0.41, 95% CI 0.20–0.62), and 1-year overall survival (OR = 0.64, 95% CI 0.48–0.86). The risk of bias across studies was considerable in the domains of allocation concealment, blinding, and intervention contamination.

Conclusions and relevance
Although we found evidence to support the intervention for highly relevant outcomes, our conclusions are tempered by the high risk of bias relating mainly to intervention design. PROM feedback for oncology patients may improve processes and outcomes for cancer patients but more high-quality evidence is required.
Comparative study between ultrasound-guided erector spinae plane block and thoracic paravertebral block for postoperative analgesia after video-assisted thoracic surgery: an equivalence study
Authors: Sobhy Ahmed Anwer , Sharaf Samia Ibrahim , Kamaly Ayman Mokhtar , Hilal Amr Mohamed , Elaziz Farouk Kamal Eldin Abd 
Published: June 2023

Abstract
Background
It is advised to use a regional block to lower postoperative opioid usage. Therefore, we aimed to compare the analgesic effect of erector spinae plane block (ESPB) and thoracic paravertebral block (TPVB) on the need for morphine following video-assisted thoracic surgery (VATS).

Results
The findings revealed that TPVB and ESPB groups were equivalent in analgesic efficacy with postoperative morphine consumption in mg 15.2 ± 2.7 and 14.9 ± 2.4 respectively. statistically significant shorter block time in ESPB was 8.1 ± 1.7 min in contrast to 11.35 ± 1.7 min in TPVB and better patient satisfaction in the ESPB than in TPVB groups (p value < 0.05). No statistically significant differences existed between the two groups in terms of postoperative pain score (VAS) at rest, cough at any time point, or incidence of complications (p value > 0.05). There were significantly lower heart rate (HR) and blood pressure (BP) trends in 15 and 30 min intraoperatively (p value < 0.001) in the TPVB group.

Conclusions
ESPB has analgesic efficacy equivalent to TPVB from aspects of equivalent postoperative morphine consumption and VAS at rest and during cough with shorter block time and better patient satisfaction.

Trial registration
Pan African Clinical Trial Registry PACTR202109527452994. Registered on 8 September 2021.
Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach
Authors: Shah Murad-Ali , Kang Ju-Bin , Koh Phil-Ok 
Published: June 2023

Abstract
Background
Cerebral ischemia is a serious neurological disorder that can lead to high morbidity and mortality. Chlorogenic acid is a polyphenol compound with antioxidant that can regulate proteins in cerebral ischemia. Middle cerebral artery occlusion (MCAO) surgery was performed to induce ischemic brain injury and was maintained for 24 h. Chlorogenic acid (30 mg/kg) or vehicle was administrated into the peritoneal cavity 2 h after MCAO surgery. The cerebral cortical tissues were collected for further study and a proteomic approach was performed to identify the proteins changed by chlorogenic acid in the MCAO animals.

Results
We found that chlorogenic acid alleviated in changes in adenosylhomocysteinase, glycerol-3-phosphate dehydrogenase, eukaryotic translation initiation factor 4A-II, apolipoprotein A-I, and mu-crystallin. These proteins were reduced in MCAO animals with vehicle, and these reductions were attenuated by chlorogenic acid treatment. The mitigation of this reduction by chlorogenic acid was confirmed by the reverse transcription PCR technique. These proteins are associated with energy metabolism, protein synthesis, inflammation, and physiological metabolism. They are involved in the neuroprotective effect of chlorogenic acid. These results showed that chlorogenic acid alleviates the neurological disorders caused by MCAO and regulates the expression of proteins involved in neuroprotection.

Conclusions
Therefore, our findings provide evidence that chlorogenic acid plays a neuroprotective role in stroke animal models by controlling specific proteins.
Defining re-implementation
Authors: Moyal-Smith Rachel , Etheridge James C. , Karlage Ami , Sonnay Yves , Yuan Christina T. , Havens Joaquim M. , Brindle Mary E. , Berry William 
Published: June 2023

Abstract
Background
The first attempt to implement a new tool or practice does not always lead to the desired outcome. Re-implementation, which we define as the systematic process of reintroducing an intervention in the same environment, often with some degree of modification, offers another chance at implementation with the opportunity to address failures, modify, and ultimately achieve the desired outcomes. This article proposes a definition and taxonomy for re-implementation informed by case examples in the literature.

Main body
We conducted a scoping review of the literature for cases that describe re-implementation in concept or practice. We used an iterative process to identify our search terms, pilot testing synonyms or phrases related to re-implementation. We searched PubMed and CINAHL, including articles that described implementing an intervention in the same environment where it had already been implemented. We excluded articles that were policy-focused or described incremental changes as part of a rapid learning cycle, efforts to spread, or a stalled implementation. We assessed for commonalities among cases and conducted a thematic analysis on the circumstance in which re-implementation occurred. A total of 15 articles representing 11 distinct cases met our inclusion criteria. We identified three types of circumstances where re-implementation occurs: (1) failed implementation, where the intervention is appropriate, but the implementation process is ineffective, failing to result in the intended changes; (2) flawed intervention, where modifications to the intervention itself are required either because the tool or process is ineffective or requires tailoring to the needs and/or context of the setting where it is used; and (3) unsustained intervention, where the initially successful implementation of an intervention fails to be sustained. These three circumstances often co-exist; however, there are unique considerations and strategies for each type that can be applied to re-implementation.

Conclusions
Re-implementation occurs in implementation practice but has not been consistently labeled or described in the literature. Defining and describing re-implementation offers a framework for implementation practitioners embarking on a re-implementation effort and a starting point for further research to bridge the gap between practice and science into this unexplored part of implementation.
Epigenetic regulation and therapeutic targets in the tumor microenvironment
Authors: Xie Zhuojun , Zhou Zirui , Yang Shuxian , Zhang Shiwen , Shao Bin 
Published: June 2023

AbstractThe tumor microenvironment (TME) is crucial to neoplastic processes, fostering proliferation, angiogenesis and metastasis. Epigenetic regulations, primarily including DNA and RNA methylation, histone modification and non-coding RNA, have been generally recognized as an essential feature of tumor malignancy, exceedingly contributing to the dysregulation of the core gene expression in neoplastic cells, bringing about the evasion of immunosurveillance by influencing the immune cells in TME. Recently, compelling evidence have highlighted that clinical therapeutic approaches based on epigenetic machinery modulate carcinogenesis through targeting TME components, including normalizing cells’ phenotype, suppressing cells’ neovascularization and repressing the immunosuppressive components in TME. Therefore, TME components have been nominated as a promising target for epigenetic drugs in clinical cancer management. This review focuses on the mechanisms of epigenetic modifications occurring to the pivotal TME components including the stroma, immune and myeloid cells in various tumors reported in the last five years, concludes the tight correlation between TME reprogramming and tumor progression and immunosuppression, summarizes the current advances in cancer clinical treatments and potential therapeutic targets with reference to epigenetic drugs. Finally, we summarize some of the restrictions in the field of cancer research at the moment, further discuss several interesting epigenetic gene targets with potential strategies to boost antitumor immunity.
Mutation Burden Independently Predicts Survival in the Pan-Cancer Atlas
Authors: Smith Jeffrey R. , Parl Fritz F. , Dupont William D. 
Published: June 2023

PURPOSE Long-standing clinical predictors of cancer survival have included histopathologic type, stage, and grade. We hypothesized that the principal categories of tumor somatic mutations might also portend survival. We investigated this hypothesis using the Pan-Cancer Atlas, encompassing clinical, genomic, and outcome data of 10,652 patients and 32 cancer types. METHODS We evaluated the prognostic capability of cancer type, stage, grade and the burden of each major mutation category on overall and disease-specific survival. Mutation categories included short substitution and insertion-deletion mutations (SMs), copy number alterations (CNAs), and gene fusions. RESULTS SM count and CNA fraction proved to be strong independent predictors of survival (joint P = 5.3e−95) that remained highly significant when adjusted for the traditional factors. Importantly, the relationship between mutation burden and survival proved to be nonlinear ( P = 9.5e−56); survival improved at both low- and high-burden extremes. In clinically predictive modeling, SM count together with CNA fraction meaningfully distinguished survival even among patients sharing a given cancer type, stage, or grade. CONCLUSION Burden of somatic mutation is a key index of survival of analogous clinical utility to these traditional factors.
Trimaximal mixing and extended magic symmetry in a model of neutrino mass matrix
Authors: Singh Labh , Tapender  , Kashav Monal , Verma Surender 
Published: June 2023

Abstract
The trimaximal mixing scheme

results in “magic” neutrino mass matrix

which is known to accommodate neutrino oscillation data. In this paper, we propose a phenomenological ansatz for

by extending the magic symmetry that leads to further reduction in the number of free parameters, thereby increasing the predictability of the model. The neutrino mixing parameters, effective Majorana mass m

ee
and CP invariants (J

CP
, I
1, I
2) are found to exhibit strong correlations for

mixing paradigm. One of the generic feature of the model is the requirement of non-maximal

for possible CP violation measurable in neutrino oscillation experiments. The observables m

ee
and sum of neutrino masses

have imperative implications for yet unknown neutrino mass hierarchy. For inverted hierarchy, the lower bound on

, predicted by the model, is found to be within the sensitivity reach of the

decay experiments. Also, cosmological bound of 0.12 eV on

, at 95% CL, refutes inverted hierarchy implying

with normal hierarchy as the only viable possibility in the model. We have, also, illustrated a scenario wherein such a construction of the neutrino mass matrix can be realized using

symmetry in the framework of Type-I+II seesaw mechanism.
Motherwork, Daughterwork
Authors: de los Reyes Paulina , Mulinari Diana 
Published: June 2023

Abstract
This article explores how young women born in Sweden, or arriving in Sweden as toddlers, who belong to the Latin American diaspora give meaning to and act on their experiences of being the daughters of migrant mothers, whose political activism shapes their views and practices of mothering and migration. The analysis is inspired by feminist/antiracist methodologies and consists of eight in-depth interviews with young adult women, all of them daughters of political refugees who came to Sweden to escape persecution by the military dictatorships in Latin America in the late 1970s. The interviews are combined with two focus groups that took place in the two largest Swedish cities. The category we will bring to light is that of the daughters; adults now, aged between thirty and forty-five (six of them mothers themselves). The central question in this article is what perceptions of mothering can be articulated from the perspective of daughters of Latin American migrant mothers, in a context where memories of political persecution and exile and experiences of institutional and everyday racism shape the conditions of motherwork for both the migrant mothers and their daughters.
Strategies of Visibilization
Authors: sandoval romero* claudia 
Published: June 2023

Abstract
Strategies of Visibilization is a spatial installation that consists of the listings of artists who participated in exhibitions at the Museum moderner Kunst Stiftung Ludwig Wien-mumok between 1998 and 2018. The installation compares proportions of participation by gender and geopolitical origin. From a subjective-critical perspective and by addressing questions about representation, positioning in the artistic field, and power relationships within the museological context, the proposal seeks to contribute to the debates about loss, mourning, and restitution of women in the Global South who have been denied a position in the art field.