bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative secondary metabolite expression in callus cultures and mother plant in Barleria prionitis L.
Authors: Ranade Rashmi , Joshi Neelu , Kudale Subhash 
Published: May 2023

Abstract

The present study is the first report on the quantitative determination of secondary metabolites in callus cultures of B. prionitis L, an important medicinal plant. We examined the stem-derived and leaf-derived callus for their antioxidant potential and accumulation of phenolics, flavonoids, and active principles viz. shanzhiside methyl ester (SME) and squalene and compared these parameters vis a vis mother plant counterpart. Four solvents viz. methanol, ethanol, acetone, and distilled water (aqueous) extracts were prepared for analysis. Callus cultures not only preserved the metabolite profile of the mother plant but also displayed their elevated levels. Leaf-derived callus surpassed stem-derived callus in most of the parameters studied. The highest phenolic content (21.46 mg GAE g− 1 FW) and flavonoid content (24.58 mg of RE g− 1 FW) was observed in methanol extract of leaf-derived callus, representing a 3-fold and 2-fold increase over mother plant leaf, respectively. Antioxidant capacity based on FRAP and DPPH assay was highest in methanol extract of leaf-derived callus (7-fold and 3-fold increase over mother plant, respectively) while ABTS assay showed the highest activity (122-fold increase) in acetone extract of leaf-derived callus. HPTLC analysis revealed an enhanced concentration of squalene (10-fold) and SME (2.3-fold) in acetone and methanol extract of leaf-derived callus, respectively, compared to mother explants. Results of RP-HPLC for phenolics showed the highest gallic acid content (99-fold increase) in ethanol extract of stem-derived callus whereas catechol was maximum (37- fold increase) in aqueous extract of leaf-derived callus. These findings suggest that callus cultures of B. prionitis can be a potential source of active metabolites. Further, cell suspension cultures can be established from the callus which could be an avenue for the large-scale production of bioactive compounds.
Measurement of superoxide dismutase: Clinical usefulness for patients with anti-neutrophil cytoplasmic antibody-associated vasculitis
Authors: Zhang Zhihuan , Huang Wenhan , Ren Feifeng , Luo Lei , Zhou Jun , Tang Lin 
Published: May 2023

Abstract
Objective
To investigate the clinical usefulness of serum superoxide dismutase (SOD) measurement in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV).
Methods
In this single-center retrospective study, demographic data, serum SOD levels, Erythrocyte sedimentation rate (ESR), C reactive protein (CRP), the Birmingham Vasculitis Activity Score (BVAS), ANCA, organ involvement, and prognosis were analyzed for 152 AAV patients hospitalized in the Second Affiliated Hospital of Chongqing Medical University. Meanwhile, the serum SOD levels of 150 healthy people were collected as the control group.
Results
Compared to the healthy control group, serum SOD levels of the AAV group were significantly lower (P < 0.001). SOD levels of AAV patients were negatively correlated to ESR, CRP, and BVAS (ESR rho = − 0.367, P < 0.001; CRP rho = − 0.590, P < 0.001; BVAS rho = − 0.488, P < 0.001). SOD levels for the MPO-ANCA group were significantly lower than the PR3-ANCA group (P = 0.045). SOD levels for the pulmonary involvement group and the renal involvement group were significantly lower than those for the non-pulmonary involvement group and the non-renal involvement group (P = 0.006; P < 0.001, respectively). SOD level in the death group were significantly lower than the survival group (P = 0.001).
Conclusions
In AAV patients, low SOD levels might indicate disease associated oxidative stress. SOD levels in AAV patients were decreased with inflammation, suggesting that SOD levels could potentially be a surrogate marker for disease activity. SOD levels in AAV patients were closely related to ANCA serology, pulmonary involvement, and renal involvement, with low SOD levels an important indicator of a poor prognosis for AAV patients.
Self-Consistency Congruence and Non-Suicidal Self-Injury in College Students: the Mediating Role of Negative Emotions and Mobile Phone Addiction
Authors: Wang Lingli , Hu Gengdan , Gao Meicai , Li Yang 
Published: May 2023

Abstract
Non-suicidal self-injury (NSSI) is a critical and widely recognized public issue that has been associated with various psychological problems, such as depression, anxiety, maladaptive behavior, and internet addiction, and is a significant predictor of suicide. In this study, we aimed to investigate the mediating effects of negative emotions and mobile phone addiction on self-consistency congruence and NSSI in college students by developing a model. The sample consisted of 1051 college students who completed the Self-Consistency Congruence Questionnaire, the NSSI Questionnaire, the Mobile Phone Addiction Questionnaire, and the Positive and Negative Emotions Questionnaire. Our findings revealed that self-consistency congruence was negatively associated with NSSI in college students. Mediation analysis demonstrated that self-consistency congruence indirectly influenced NSSI among college students through negative emotions and mobile phone addiction. These results suggest that self-consistency congruence can decrease the likelihood of NSSI by reducing negative emotions and mobile phone addiction.
Quantum annealing of a well-defined frustrated magnet
Authors: Li Yuesheng , Zhao Yuqian , Ma Zhaohua , He Zhangzhen , Liao Haijun , Wang Junfeng 
Published: May 2023

Abstract

Quantum annealing, which involves quantum mechanical tunnelling among possible solutions, has state-of-the-art applications not only in quickly finding the lowest-energy configuration of a complex system [1, 2], but also in quantum computing [3, 4]. Despite recent progress in quantum simulators, processors, and algorithms [5, 9], well-defined materials that exhibit many-body quantum annealing phenomena are still unavailable in the real world. Here we report a single-crystal study of spin dynamics and heat-transport properties of the frustrated magnet α-CoV2O6, consisting of a triangular arrangement of ferromagnetic Ising spin chains without evident structural disorder [10-12]. We observe apparent quantum annealing phenomena resulting from time-reversal symmetry breaking in a tiny transverse field. For instance, below～ 1 K and in a longitudinal magnetic field of～2 T, the system exhibits no indication of approaching the lowest-energy state for at least 15 hours in zero transverse field, but quickly converges towards that configuration with a nearly temperature-independent relaxation time of ~10 seconds in a transverse field of ~3.5 mK. Our large-scale classical and quantum Monte Carlo simulations show good agreement with the experimental results, and suggest that a tiny transverse field can profoundly enhance quantum spin fluctuations around domain walls, triggering rapid quantum annealing process from metastable Kosterlitz-Thouless phases, at low temperatures.
Analyzing hydro-meteorological data from Yamuna river basin, western Himalaya: Using a Markov Chain and LSTM approach to forecast future disasters
Authors: chauhan pankaj , Akiner Muhammed Ernur , Sain Kalachand 
Published: May 2023

Abstract
This research aims to evaluate hydro-meteorological data from the discharge site at the Dakpathar barrage in the Yamuna River basin, Uttarakhand, India, utilizing Extreme Value Distribution of Frequency Analysis and the Markov Chain Approach. This method assesses persistence and allows for combinatorial probability estimations such as initial and transitional probabilities. The hydrologic data was generated (in-situ) and received from Uttarakhand Jal Vidut Nigam Limited (UJVNL), and meteorological data was acquired from NASA's archives MERRA-2 product. A total of sixteen years (2005-2020) of data was used to foresee daily precipitation from 2020 to 2022. Precipitation data for 2021 and 2022 were also retrieved from MERRA-2 products and utilized as observed and forecast values for daily precipitation throughout the monsoon season, which runs from July to September. Markov Chain and Long Short-Term Memory (LSTM) findings for 2020, 2021, and 2022 were observed and anticipated values for daily rainfall during the monsoon season between July and September. According to test findings, the artificial intelligence technique cannot anticipate future regional meteorological formations; the correlation coefficient R2 is around 0.12. According to the randomly verified precipitation data findings, the Markov Chain model has a success rate of 79.17 percent. The results suggest that extended return periods should be seen as a warning sign for drought and flood risk in the Himalayan region. This study gives a better knowledge of the water budget and better evaluations of the overall state of the climate change variability, impact for global warning, ultimately leading to improved water resource management and better emergency planning to establishment of the Early Warning System (EWS) for extreme occurrences such as cloudbursts, flash floods, landslides and other hydro-meteorological related hazards in the complex Himalayan region.
Assessment of genotypic variability and Genome Wide Association studies for cooking time and canning quality traits in common bean ( Phaseolus vulgaris L.)
Authors: Kesiime Eunice Vasiter , Nkalubo Stanley Tamusange , Ochwo Mildred Ssemakula , Dramadri Isaac Onziga , Mukankusi Clare , Nakimbugwe Dorothy , Edema Richard , Gibson Paul , Badji Arfang 
Published: May 2023

AbstractDeveloping common bean genotypes that possess short cooking time and good traits for canning is very key in accelerating bean consumption among both the urban poor and the middle class population. With the objective of assessing the genotypic variability and with Genome wide association studies (GWAS) identify SNP markers associated with the two traits, 250 common bean accessions were evaluated using alpha lattice design with two replications and two seasons. Three months post-harvest, the bean grains were evaluated for the two traits. Analysis from mixed models revealed significant variation for both traits among the 250 accessions tested. Cooking time ranged from 51.56 to 215 minutes, with 11 genotypes cooking in less than 60 minutes and total canning quality score ranged from 41.3 to 84% with 31 genotypes scoring 76% and above. GWAS conducted on 217genotypes revealed a total of 47 significant marker trait associations for the two traits; on chromosome Pv01 for cooking time(CT) and washed drain coefficient (WDC), viscosity, WDC, washed drain solids(WDS), splitting and hydration coefficient(HC) on chromosome Pv02, WDS, HC, free starch(FS) and splitting on Pv03, CT, WDC and WDS on Pv04, viscosity, splitting, FS, colour and CT on Pv05. Clumping on Pv06, visual appeal, colour and viscosity on Pv07, visual appeal, clumping, splitting and WDC on Pv08, clumping, viscosity, FS and on Pv09, colour, FS, WDS and clumping on Pv010, CT, FS, clumping, viscosity and WDS on Pv011. Possible candidate genes including; phvul.007G2803001 and Phvul.007G280700.2 both on chromosome 7 and Phvul.005G161200.1 on chromosome 5, all associated with seed coat colour were identified. Besides their usefulness in breeding research, these studies identified genotypes whose shorter cooking time and good canning quality traits could benefit the consumption and processing industries.
A Novel NODAL variant in a young embolic stroke patient with visceral heterotaxy
Authors: Kaburagi Kei , Hagiwara Yuta , Tachikawa Keiji , Miyake Noriko , Akiyama Hisanao , Kawai Yosuke , Omae Yosuke , Tokunaga Katsushi , Yamano Yoshihisa , Shimizu Takahiro , Mitsuhashi Satomi 
Published: May 2023

Abstract
Background:
Ischemic stroke in young adults can be caused by a variety of etiologies including the monogenic disorders. Visceral heterotaxy is a condition caused by abnormal left-right determinations during embryonic development. We aimed to determine the cause of a young ischemic stroke patient with visceral heterotaxy.
Case presentation:
We performed neurological, radiological, and genetic evaluations in a 17-year-old male patient presenting ischemic stroke and visceral heterotaxy to determine the underlying cause of this rare disease combination. Brain MRI showed evidence of embolic stroke, abdominal CT showed visceral heterotaxy, and echocardiogram showed cardiac anomaly with right-to-left-shunt (RLS). Whole genome sequencing (WGS) revealed a heterozygous missense variant (NM_018055.5: c.1016T > C, p.(Met339Val)) in the NODAL gene, which is essential to the determination of the left-right body axis.
Conclusions:
Our study highlights the importance of evaluating genetic causes in young ischemic stroke of unknown cause and the need for stroke risk management in visceral heterotaxy patients with RLS. To the best of our knowledge, we report the first genetically-confirmed case of visceral heterotaxy with young embolic stroke reported to date.
Epigenetic priming targets tumor heterogeneity to shift transcriptomic phenotype of pancreatic ductal adenocarcinoma towards a Vitamin D susceptible state
Authors: He Bo , Stoffel Lauren , He Clifford , Cho Kumsun , Li Albert , Jiang Haowen , Flowers Brittany , Ferreira Sofia , Zhou Meng-Ning , Attardi Laura , Ye Jiangbin 
Published: May 2023

Abstract
As a highly heterogeneous tumor, pancreatic ductal adenocarcinoma (PDAC) exhibits non-uniform responses to therapies across subtypes. Overcoming therapeutic resistance due to tumor heterogeneity in PDAC remains a challenge. Here, we report that Vitamin D-resistant PDAC cells hijacked Vitamin D signaling to promote tumor progression, whereas epigenetic priming with glyceryl triacetate (GTA) and 5-Aza-2′-deoxycytidine (5-Aza) overcame Vitamin D resistance and shifted the transcriptomic phenotype of PDAC toward a Vitamin D-susceptible state. Increasing overall H3K27 acetylation with GTA and reducing overall DNA methylation with 5-Aza not only elevated the Vitamin D receptor (VDR) expression but also reprogrammed the Vitamin D-responsive genes. Consequently, Vitamin D inhibited cell viability and migration in the epigenetically primed PDAC cells by activating genes involved in apoptosis as well as genes involved in negative regulation of cell proliferation and migration, while the opposite effect of Vitamin D was observed in unprimed cells. Studies in genetically engineered mouse PDAC cells further validated the effects of epigenetic priming for enhancing the anti-tumor activity of Vitamin D. Using gain- and loss-of-function experiments, we further demonstrated that VDR was necessary for augmenting original Vitamin D responses, but was insufficient for shifting transcriptomic phenotype of PDAC toward a therapeutic response to Vitamin D, highlighting that both the VDR and Vitamin D-responsive genes were prerequisites for Vitamin D response. These data reveal a previously undefined mechanism in which epigenetic state orchestrates the expression of both VDR and Vitamin D-responsive genes, and determines the response to Vitamin D in PDAC.
Research on the Coordinated Development Path of Green Economy in Beijing-Tianjin-Hebei Urban Agglomeration
Authors: WANG Sha , Yude HE , JIANG Yuguo 
Published: May 2023

Abstract
Green development is a model of coordinated and sustainable economic growth and social development. Improving the level of green coordinated development in a region is a basic requirement for urban sustainable development. The Beijing-Tianjin-Hebei region is an important core urban agglomeration for economic development in northern China.Taking the Beijing-Tianjin-Hebei region as an example, this study uses environmental data from 2011 to 2020 to assess the level of green coordinated development between industrial upgrading and the ecological environment based on the theory of coordinated development. And use the system dynamics method to explore the important factors affecting the green coordination in the study area. The main results are as follows: 1. During the research period, the degree of green coordination in the study area has been continuously improved, the growth trend of industrial development is more obvious, and the improvement of the ecological environment is not obvious.2.The gap between Beijing, Tianjin, and Hebei has been further widened, Beijing is at a high level of coordination, while Tianjin and Hebei are at a low level of coordination.3.Reducing energy consumption and increasing the labor force in the tertiary industry are both important factors affecting the level of green coordinated development in a region, Moreover, the reduction in energy consumption has a more significant effect on improving the coordination level.
Preparation and characterization of poly(butylene succinate-co-adipate)/poly(lactic acid) blends with enhanced compatibility, mechanical properties, and heat resistance
Authors: Liu Chengkai , Li Chentao , Hao Yanping , Yan Xiangyu , Deng Huiwen , Pan Hongwei , Bian Junjia , Wang Dongmei , Zhao Yan , Zhang Huiliang 
Published: May 2023

AbstractIn this study, an environmental friendly poly(butylene succinate-co-adipate) (PBSA)/ poly(lactic acid) (PLA) blend was prepared with 1,4-bis(tert-butyl peroxy isopropyl) benzene (BIPB) as a reactive compatibilizer. Rheological analysis and gel permeation chromatography analysis showed that the addition of BIPB increased the melt strength and molecular weight of PBSA/PLA blends by reacting with PBSA and PLA during the melt-blending process. The morphology of the cryo-fractured surfaces of PBSA/PLA/BIPB blends showed that adding BIPB could significantly enhance the compatibility between PBSA and PLA. Moreover, the tensile tests showed that adding BIPB could improve the tensile strength, Young's modulus and breaking strain of PBSA/PLA blends. Furthermore, the effect of isothermal treatment on PBSA/PLA/BIPB blends was also studied. WAXD and DSC curves showed that the isothermal treatment could obviously increase the crystallinity of PLA and then increase the heat deflection temperatures of PBSA/PLA/BIPB blends. In addition, adding PLA could significantly improve the hydrolytic properties of PBSA/PLA/BIPB blends.
Dual Modulation Strategy of Mn Donor Doping and Se Vacancy Endows Large Capacity and High-Rate Charge Storage in a Ni3Se4 Battery-Type Cathode
Authors: zhao Jian , He Yinna , Zhang Yuxiao , Song Jiangnan , Wang Yiwei , Feng Jie , Yang Lina , Gao Ge , Liu Ting , Cheng He , Meng Alan , Li Guicun , Wang Lei , Li Zhenjiang 
Published: May 2023

AbstractTransition metal selenides (TMSs) have been extensively explored as the most attractive battery-type supercapacitor cathodes. However, TMSs usually exhibit elusive active sites, sluggish reaction/diffusion kinetics, and poor conductivity, which severely degrade their capacity and rate performance. To overcome these shortcomings of TMSs, herein, we employ a synchronous strategy of Mn donor doping and Se vacancy in a flower-like Ni3Se4cathode and optimize the dopant/vacancy concentration (VSe(M)–Mn(M)–Ni3Se4) to maximize electron release from Mn and effectively accumulate the electrons around the vacancy, resulting in maximum electron transfer during the charge–discharge process. Moreover, the proposed strategy can intrinsically tune electronic structure, increase electroactive sites, accelerate OH−diffusion kinetics, and pledge the ion chemisorption–desorption equilibrium verified based on first-principle calculations. Thus, the fabricated cathode exhibits ultrahigh capacity and rate capability (357 and 275 mAh g−1at 1 and 100 A g−1, respectively), and a hybrid supercapacitor with the cathode exhibits sufficient energy density of 118 Wh kg−1at 0.8 kW kg−1and exceptional durability, which is considerably greater than that exhibited by supercapacitors comprising other cathodes. Moreover, the charge–discharge mechanism is elaborated in detail via ex situ techniques. This study provides fundamental guidelines for constructing high-performance battery-type cathodes, which can be used in next-generation supercapacitors.
Effects of mulched drip irrigation and biochar on nitrogen accumulation, yield, water use efficiency, quality, and soil properties of peanut
Authors: Zhang Junxiao , Sun Yidi , Liu Xiaowei , Huang Zhenyu , Wu Qi , Xia Guimin , Chi Daocai 
Published: May 2023

Abstract
Field experiments were carried out in Western Liaoning Province to investigate the effects of biochar on peanut nitrogen accumulation (NA) and soil properties in aeolian sandy soil and the relationship between NA parameters regulated by biochar and peanut quality. A split-plot design was adopted. Drip irrigation and mulched drip irrigation were used in the main plot, and three biochar application rates of B0 (0 t/ha), B1 (24 t/ha), and B2 (48t/ha) were used in the sub-plots. The results showed that different irrigation methods and biochar application significantly increased NA of peanuts, and NA of peanuts was in line with a logistic growth function (R2༞0.97). In 2020 and 2021, the pod NA of MB1 compared with MB0 treatment increased by 52.28% and 32.58% at pod filling stage, respectively. B2 treatment increased soil organic matter by 109.66%, cation exchange capacity by75.94%, total N content by 5% (P༞0.05), and pH by 15.24% compared with no biochar. The correlation coefficient between soil total N and protein content was 0.73, and that between soil pH and oil was 0.83. Compared with MB2 and DB0, MB1 treatment increased yield by 62.50% and 38.30%, respectively. In conclusion, applying biochar (B1) to a peanut cultivation system in aeolian sandy soil area increased peanut NA, improved soil properties related to peanut quality, broke through environmental barriers and improved peanut quality, which is important for peanut N fixation and improving quality in the semi-arid aeolian sandy soil area of western Liaoning Province.
Host factors underlying genetic susceptibility to Xanthomonas infection: a study of a neglected tropical disease in passion fruit (Passiflora alata)
Authors: Cardoso Jéssica Luana Souza , Costa Zirlane Portugal da , de Carvalho Lucas Amoroso Lopes , de Souza Alessandra Alves , Pinheiro Daniel Guariz , Vieira Maria Lucia Carneiro 
Published: May 2023

Abstract
De novo RNA-Seq assembly facilitates the study of transcriptomes of non-model, underutilized crops, enabling researchers to capture the maximum number of genes expressed in plant tissues. We were able to describe the expression profiling of the sweet passion fruit (Passiflora alata) in response to Xanthomonas axonopodis pv. passiflorae(Xap) infection. The crop is appreciated for the typical aroma and characteristic flavor of its fruits. However, yield is impaired by Xap, whose effects are exacerbated by high temperature and humidity. Initially, we provided the P. alata transcriptome assemblies which were shown to have high completeness, based on the expected gene content for a de novo transcriptome assembly. A total of 1,329 were completed genes and 96.6% of the orthologs conserved across Embryophytes were represented in the assembled transcriptome. Genes involved in pathogen recognition such as PRRs, R genes and genes related to the signaling cascade, coding for specific transcription factors and secondary metabolites, were found to be upregulated after infection. P. alata is known to be susceptible to Xap, thus we were interested in identifying possible susceptibility (S) genes. Interestingly, both characterized S genes in other plant species i.e., SWEET10 and LOB1were found to be upregulated in P. alata, suggesting that an effector-triggered susceptibility was achieved through the interaction between Xap and P. alata. Our qPCR results corroborate the role played by these genes, which could potentially be targets for genome editing in order to produce disease-resistant cultivars.