bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiomic interpretation of fungus-infected ant metabolomes during manipulated summit disease
Authors: Will I. , Attardo G. M. , de Bekker C. 
Published: September 2023

AbstractCamponotus floridanusants show altered behaviors followed by a fatal summiting phenotype when infected with manipulatingOphiocordyceps camponoti-floridanifungi. Host summiting as a strategy to increase transmission is also observed with parasite taxa beyond fungi, including aquatic and terrestrial helminths and baculoviruses. The drastic phenotypic changes can sometimes reflect significant molecular changes in gene expression and metabolite concentrations measured in manipulated hosts. Nevertheless, the underlying mechanisms still need to be fully characterized. To investigate the small molecules producing summiting behavior, we infectedC. floridanusants withO. camponoti-floridaniand sampled their heads for LC–MS/MS when we observed the characteristic summiting phenotype. We link this metabolomic data with our previous genomic and transcriptomic data to propose mechanisms that underlie manipulated summiting behavior in “zombie ants.” This “multiomic” evidence points toward the dysregulation of neurotransmitter levels and neuronal signaling. We propose that these processes are altered during infection and manipulation based on (1) differential expression of neurotransmitter synthesis and receptor genes, (2) altered abundance of metabolites and neurotransmitters (or their precursors) with known behavioral effects in ants and other insects, and (3) possible suppression of a connected immunity pathway. We additionally report signals for metabolic activity during manipulation related to primary metabolism, detoxification, and anti-stress protectants. Taken together, these findings suggest that host manipulation is likely a multi-faceted phenomenon, with key processes changing at multiple levels of molecular organization.
Last glacial hydroclimate variability in the Yucatán Peninsula not just driven by ITCZ shifts
Authors: Travis-Taylor Leah , Medina-Elizalde Martín , Karmalkar Ambarish V. , Polanco-Martinez Josué , Serrato Marks Gabriela , Burns Stephen , Lases-Hernández Fernanda , McGee David 
Published: September 2023

AbstractWe reconstructed hydroclimate variability in the Yucatán Peninsula (YP) based on stalagmite oxygen and carbon isotope records from a well-studied cave system located in the northeastern YP, a region strongly influenced by Caribbean climate dynamics. The new stalagmite isotopic records span the time interval between 43 and 26.6 ka BP, extending a previously published record from the same cave system covering the interval between 26.5 and 23.2 ka BP. Stalagmite stable isotope records show dominant decadal and multidecadal variability, and weaker variability on millennial timescales. These records suggest significant precipitation declines in the broader Caribbean region during Heinrich events 4 and 3 of ice-rafted discharge into the North Atlantic, in agreement with the antiphase pattern of precipitation variability across the equator suggested by previous studies. On millennial timescales, the stalagmite isotope records do not show the distinctive saw-tooth pattern of climate variability observed in Greenland during Dansgaard–Oeschger (DO) events, but a pattern similar to North Atlantic sea surface temperature (SST) variability. We propose that shifts in the mean position of the Intertropical Convergence Zone (ITCZ), per se, are not the dominant driver of last glacial hydroclimate variability in the YP on millennial timescales but instead that North Atlantic SSTs played a dominant role. Our results support a negative climate feedback mechanism whereby large low latitude precipitation deficits resulting from AMOC slowdown would lead to elevated salinity in the Caribbean and ultimately help reactivate AMOC and Caribbean precipitation. However, because of the unique drivers of future climate in the region, predicted twenty-first century YP precipitation reductions are unlikely to be modulated by this negative feedback mechanism.
Long-term efficacy and safety of adjunctive perampanel in pediatric patients aged 4–19 years with epilepsy: a real-world study
Authors: Youn Song Ee , Kang Hoon-Chul , Lee Joon Soo , Kim Heung Dong , Kim Se Hee 
Published: September 2023

AbstractThis study determined the 24-month outcomes of perampanel treatment in children and adolescents with epilepsy. The percentage of ≥ 50% responders was 47.3% (139/294) at 12 months and 49.0% (144/294) at 24 months. A 100% reduction in seizures for more than 12 months was observed in 12.2% (36/294). Discontinuation occurred in 39.8% (117/294). The most common reason for discontinuation was adverse events (29.1%, 34/117). Baseline seizure frequency was higher in children aged < 12 years than in patients aged ≥ 12 years; however, the percentage of seizure reduction and ≥ 50% responders did not significantly differ between the two groups. The rate of early discontinuation was higher (p < 0.001) and the duration of perampanel treatment was shorter in children aged < 12 years (p = 0.001). Most children aged < 12 years discontinued PER due to inadequate effectiveness, while adverse event was the most common reason in patients aged ≥ 12 years (p = 0.045). Only slow titration was significantly associated with ≥ 50% of responders. In conclusion, this study showed that perampanel can be utilized effectively and safely for a prolonged period in pediatric patients aged 4 to < 12 years, as well as in patients aged 12 years and older.
Prognostic impact of radiotherapy-induced-lymphopenia in patients treated with breast-conservative surgery
Authors: Yoon Chang Ik , Hwang Jawon , Kim Dooreh , Ji Jung Hwan , Lee Janghee , Bae Soong June , Jeong Joon , Chang Jee-Suk , Cho Yeona , Lee Hye Sun , Kim Jee Ye , Ahn Sung Gwe 
Published: September 2023

AbstractWe investigated a prognostic impact of radiotherapy-induced lymphopenia (RIL) in breast cancer patients treated with breast-conservative surgery (BCS). We included 531 breast cancer patients who were treated with BCS and adjuvant radiotherapy. None of these received (neo)adjuvant chemotherapy. Pre- and post- absolute lymphocyte counts (ALC) were reviewed before and after radiotherapy. The primary endpoint was to evaluate recurrence-free survival (RFS) according to the pre-to-post ALC ratio. Binary logistic regression model was used to identify risk factors for RIL. Either continuous or categorical (> 2.4) pre-to-post ALC ratio was associated with RFS. In 531 patients receiving whole breast irradiation (WBI) and regional nodal irradiation (RNI), RFS was significantly reduced in the patients with high pre-to-post ALC ration (> 2.4). In multivariable analysis, low pre-to-post post ALC ratio was significantly related to decreased RFS in the multivariable analysis (HR 2.293, 95% CIs 1.110–4.735, P = 0.025). In 452 patients treated with WBI alone, high pre-to-post ALC ratio was still significantly associated with decreased RFS in the multivariable analysis (HR 2.708, 95% CIs 1.016–7.218, P = 0.046). In binary logistic regression analysis, RNI was only significant risk factor for clinically meaningful RIL. Our findings show that a markedly decrease in ALC during radiotherapy has a negative prognostic impact.
Interactions of the male contraceptive target EPPIN with semenogelin-1 and small organic ligands
Authors: Gomes Antoniel A. S. , Santos Natália C. M. , Rosa Leonardo R. , Borges Rafael J. , Fontes Marcos R. M. , Hamil Katherine G. , O’Rand Michael G. , Silva Erick J. R. 
Published: September 2023

AbstractNovel male contraceptives will promote gender equality in sharing contraceptive responsibility. The sperm-associated protein epididymal protease inhibitor (EPPIN) is a promising target for non-hormonal male contraception. EPPIN interacts with the semen coagulum protein semenogelin-1 (SEMG1) on the sperm surface, leading to transient inhibition of sperm motility after ejaculation. Small organic molecules targeting EPPIN's SEMG1-binding are under development as male contraceptives. Here, we combined computational approaches to uncover key aspects underlying EPPIN binding to SEMG1 and small organic ligands. We generated a human EPPIN model showing a typical arrangement of the WFDC (Whey-acid four disulfide core)-type and Kunitz-type domains, connected by a hinge region. Determining the EPPIN model's intrinsic motion by molecular dynamics simulations and normal mode analysis revealed a conformation, presenting a binding pocket that accommodates SEMG1Glu229-Gln247, EP055, and EP012. EPPIN's residues Phe63 and Lys68 (WFDC domain), Asp71 (hinge region), and Asn113, Asn114, and Asn115 (Kunitz domain) were identified as hot spots for SEMG1, EP055, and EP012 binding. Moreover, hydrophobic and hydrophilic residues in the WFDC and Kunitz domains allow plasma membrane anchoring, orienting the EPPIN binding pocket to the solvent. Targeting EPPIN's essential residues for its biomolecular interactions may improve the rational design of EPPIN ligands as spermiostatic compounds.
The hemodynamics of adrenal veins with four-dimensional computed tomography using quantitative time-density curve: a study based on aldosteronism patients
Authors: He Xi , Sueyoshi Eijun , Nagayama Hiroki , Koike Hirofumi , Uetani Masataka 
Published: September 2023

AbstractPresent study quantitatively analyzed adrenal venous flow using four-dimensional computed tomography (4D CT). We reviewed 4D CT images of 55 patients [mean age, 52 years ± 11 (standard deviation); 23 females] who underwent adrenal venous sampling between August 2017 and February 2021. Time–density curves were referred for the adrenal venous enhancement. The clinical factors affecting hemodynamics were assessed using uni- and multivariate linear regression analyses. The right and left adrenal veins (RAV and LAV, respectively) were visualized in all cases. Mean peak enhancement values in RAV and LAV were 247 ± 67 and 292 ± 70 Hounsfield units (P < 0.01), and were reached at 44.43 ± 6.86 and 45.39 ± 7.53 s (P < 0.01), respectively. The body mass index (BMI), plasma renin activity and potassium were significant factors influencing the peak enhancement of RAV blood flow [standardized regression coefficients, − 0.327 (P = 0.017), − 0.346 (P = 0.013), 0.426 (P = 0.016), respectively]. A linear relationship between sex and the time-to-peak was observed for RAV [standardized regression coefficient, 0.348 (P = 0.046)]. RAV had a lower contrast effect than LAV and reached its peak faster. BMI, plasma renin activity, and potassium were associated with flow density in RAV. Sex independently influenced the time-to-peak.
Cost-effective, portable, patient-dedicated three-dimensional automated breast ultrasound for point-of-care breast cancer screening
Authors: Park Claire Keun Sun , Trumpour Tiana , Aziz Amal , Bax Jeffrey Scott , Tessier David , Gardi Lori , Fenster Aaron 
Published: September 2023

AbstractBreast cancer screening has substantially reduced mortality across screening populations. However, a clinical need persists for more accessible, cost-effective, and robust approaches for increased-risk and diverse patient populations, especially those with dense breasts where screening mammography is suboptimal. We developed and validated a cost-effective, portable, patient-dedicated three-dimensional (3D) automated breast ultrasound (ABUS) system for point-of-care breast cancer screening. The 3D ABUS system contains a wearable, rapid-prototype 3D-printed dam assembly, a compression assembly, and a computer-driven 3DUS scanner, adaptable to any commercially available US machine and transducer. Acquisition is operator-agnostic, involves a 40-second scan time, and provides multiplanar 3D visualization for whole-breast assessment. Geometric reconstruction accuracy was evaluated with a 3D grid phantom and tissue-mimicking breast phantoms, demonstrating linear measurement and volumetric reconstruction errors < 0.2 mm and < 3%, respectively. The system’s capability was demonstrated in a healthy male volunteer and two healthy female volunteers, representing diverse patient geometries and breast sizes. The system enables comfortable ultrasonic coupling and tissue stabilization, with adjustable compression to improve image quality while alleviating discomfort. Moreover, the system effectively mitigates breathing and motion, since its assembly affixes directly onto the patient. While future studies are still required to evaluate the impact on current clinical practices and workflow, the 3D ABUS system shows potential for adoption as an alternative, cost-effective, dedicated point-of-care breast cancer screening approach for increased-risk populations and limited-resource settings.
CD31 defines a subpopulation of human adipose-derived regenerative cells with potent angiogenic effects
Authors: Dhumale Pratibha , Nielsen Jakob Vennike , Hansen Anne Cathrine Schmidt , Burton Mark , Beck Hans Christian , Jørgensen Mads Gustaf , Toyserkani Navid Mohamadpour , Haahr Martha Kirstine , Hansen Sabrina Toft , Lund Lars , Thomassen Mads , Sørensen Jens Ahm , Andersen Ditte Caroline , Jensen Charlotte Harken , Sheikh Søren Paludan 
Published: September 2023

AbstractCellular heterogeneity represents a major challenge for regenerative treatment using freshly isolated Adipose Derived Regenerative Cells (ADRCs). Emerging data suggest superior efficacy of ADRCs as compared to the ex vivo expanded and more homogeneous ADRCs (= ASCs) for indications involving (micro)vascular deficiency, however, it remains unknown which ADRC cell subtypes account for the improvement. Surprisingly, we found regarding erectile dysfunction (ED) that the number of injected CD31+  ADRCs correlated positively with erectile function 12 months after one bolus of autologous ADRCs. Comprehensive in vitro and ex vivo analyses confirmed superior pro-angiogenic and paracrine effects of human CD31+ enriched ADRCs compared to the corresponding CD31− and parent ADRCs. When CD31+, CD31− and ADRCs were co-cultured in aortic ring- and corpus cavernous tube formation assays, the CD31+  ADRCs induced significantly higher tube development. This effect was corroborated using conditioned medium (CM), while quantitative mass spectrometric analysis suggested that this is likely explained by secretory pro-angiogenic proteins including DKK3, ANGPT2, ANAX2 and VIM, all enriched in CD31+  ADRC CM. Single-cell RNA sequencing showed that transcripts of the upregulated and secreted proteins were present in 9 endothelial ADRC subsets including endothelial progenitor cells in the heterogenous non-cultured ADRCs. Our data suggest that the vascular benefit of using ADRCs in regenerative medicine is dictated by CD31+  ADRCs.
Lactiplantibacillus plantarum as a novel platform for production and purification of integral membrane proteins using RseP as the benchmark
Authors: Kristensen Sofie S. , Lukassen Marie V. , Siebenhaar Suzana , Diep Dzung B. , Morth J. Preben , Mathiesen Geir 
Published: September 2023

AbstractThe present study describes a detailed procedure for expressing and purifying the integral membrane protein RseP using the pSIP system and Lactiplantibacillus plantarum as an expression host. RseP is a membrane-bound site-2-protease and a known antibacterial target in multiple human pathogens. In the present study, we screened five RseP orthologs from Gram-positive bacteria and found RseP from Enterococcus faecium (EfmRseP) to yield the highest protein levels. The production conditions were optimized and EfmRseP was purified by immobilized metal ion affinity chromatography followed by size-exclusion chromatography. The purification resulted in an overall yield of approximately 1 mg of pure protein per 3 g of wet-weight cell pellet. The structural integrity of the purified protein was confirmed using circular dichroism. We further assessed the expression and purification of RseP from E. faecium in the Gram-negative Escherichia coli. Detection of soluble protein failed in two of the three E. coli strains tested. Purification of EfmRseP expressed in E. coli C43(DE3) resulted in a protein with lower purity compared to EfmRseP expressed in L. plantarum. To our knowledge, this is the first time L. plantarum and the pSIP expression system have been applied for the production of membrane proteins.
Tailoring D–π–A architectures with hybridized local and charge transfer fluorophores exhibiting high electroluminescence exciton utilization and low threshold amplified spontaneous emission
Authors: Ma Lin , Yu Yue , Zhong Daokun , Zhu Chunrong , Yang Xiaolong , Feng Zhao , Zhou Guijiang , Wu Zhaoxin 
Published: September 2023

For the organic laser materials which could both use 25% singlet and 75% triplet excitons as the energy source, there’s a trade-off between the high radiative rate of the local-excited state and high exciton utilization of the charge-transfer state.
Effects of Auditory Training in Older Adults
Authors: Lai C.-Y. Yvonne , Ng P. S. , Chan Alice H. D. , Wong Francis C. K. 
Published: September 2023

Purpose:
This study examines the effects of an auditory training program on the auditory and cognitive abilities of older adults. Auditory rehabilitation programs are generally designed for hearing aid users, and studies have demonstrated benefits for them. In this study, we seek to understand whether such a training program can also benefit older adults who do not wear hearing aids. We also examined if cognitive benefits can indeed be observed as a result of the training.

Method:

Sixty-four older adults were recruited and assigned into three groups: the experimental group (
n
= 20), the active control group (
n
= 21), and the no-training control group (
n
= 23). The experimental group underwent an auditory training program (Listening and Communication Enhancement [LACE]) during the training phase. Meanwhile, the active control group listened to short audio clips and the no-training control group did not participate in any program. An auditory test (Quick Speech-in-Noise [QuickSIN]) and a battery of cognitive tests were conducted before and after the training to examine the participants' performance on auditory ability, short-term memory, and attention.

Results:
The results showed improvements in auditory and cognitive abilities during the training period. When assessing the training effects by comparing the pre- and the posttraining performances, a significant improvement on the QuickSIN task was found in the training group but not in the other two groups. However, other cognitive tests did not show any significant improvement. That is, the LACE training did not benefit short-term memory and attention. The improved performance on short-term memory during the training was not maintained in the posttraining session.

Conclusion:
Overall, the study has extended the auditory benefit from the LACE training to the typical aging population in terms of improved communication ability, but the effect of training on auditory abilities did not transfer to gains in cognitive abilities.
The Effect of Hemodilution on Cold Agglutinins
Authors: Gallimore Kimberly S. , Maurer William G. 
Published: September 2023

(J. Extra-Corpor. Technol. 19[3] p. 290-296 Fall 1987, 14 ref.) The effect of hypothermia in patients with cold agglutinin disease was studied in a patient with a strongly positive agglutination at 4°C (1:16,000 titer, a thermal amplitude of 30°C or less, and a specific I antibody). The study investigates a method of hemodilution by which patients with cold agglutinin disease may safely undergo hypothermia during cardiopulmonary bypass. By diluting the blood with an isotonic solution, the tendency toward agglutination of red blood cells is reduced, along with the risks associated with hypothermia in the patient with cold agglutinin disease.
It was concluded that as the degree of hemodilution of the blood is increased, hemodilution reduced the tendency toward aggregation of red blood cells during hypothermia when cold agglutinins are present in the blood. As a result, hemodilution may enable a patient with cold agglutinin disease to safely undergo cold cardioplegia and cardiac arrest during hypothermic conditions.
Automatic tool to annotate smile intensities in conversational face-to-face interactions
Authors: Rauzy Stéphane , Amoyal Mary 
Published: September 2023

AbstractThis study presents an automatic tool that allows to trace smile intensities along a video record of conversational face-to-face interactions. The processed output proposes a sequence of adjusted time intervals labeled following theSmiling Intensity Scale(Gironzetti, Attardo, and Pickering, 2016), a 5 levels scale varying from neutral facial expression to laughing smile. The underlying statistical model of this tool is trained on a manually annotated corpus of conversations featuring spontaneous facial expressions. This model will be detailed in this study. This tool can be used with benefits for annotating smile in interactions. The results are twofold. First, the evaluation reveals an observed agreement of 68% between manual and automatic annotations. Second, manually correcting the labels and interval boundaries of the automatic outputs reduces by a factor 10 the annotation time as compared with the time spent for manually annotating smile intensities without pretreatment. Our annotation engine makes use of the state-of-the-art toolbox OpenFace for tracking the face and for measuring the intensities of the facial Action Units of interest all along the video. The documentation and the scripts of our tool, the SMAD software, are available to download at the HMAD open source project URL pagehttps://github.com/srauzy/HMAD(last access 31 July 2023).
Subtitlers’ beliefs about pivot templates
Authors: Valdez Susana , Pięta Hanna , Torres-Simón Ester , Menezes Rita 
Published: September 2023

Abstract
Streaming service platforms are said to increase worldwide access to peripheral languages, often via the use of pivot templates. To shed light on how pivot subtitling practices impact language hierarchies and translation quality, we report on the results of an online questionnaire completed by European subtitlers. The questionnaire elicited data on the respondents’ experiences and expectations when translating from pivot templates for streaming services and other media environments (such as cable TV, cinema, and websites). The questionnaire was completed by 370 subtitlers and the elicited data were analysed quantitatively and qualitatively. The results suggest that streaming platforms reinforce traditional language hierarchies by strengthening the position of English as a hyper-central language (Heilbron 2010). ‘Peripheral–peripheral’ subtitling practices (e.g., Korean–Danish) occur mainly through pivot templates in English, and so do ‘central–central’ subtitling practices (e.g., German–French). This means that even when the original content is in a language other than English, English is still the most common source language for subtitlers because of the use of pivot templates. Furthermore, according to our respondents, pivot templates are more common in streaming platforms than in other media environments. The use of pivot templates is also reported to negatively impact subtitlers’ working conditions and give rise to particular ethical, linguistic, and technological challenges for which there are currently few guidelines and training opportunities.