bluuebunny/crossref_metadata_2025_split huggingface dataset:
NK cells encapsulated in micro/macropore-forming hydrogels via 3D bioprinting for tumor immunotherapy
Authors: Kim Dahong , Jo Seona , Lee Dongjin , Kim Seok-Min , Seok Ji Min , Yeo SeonJu , Lee Jun Hee , Lee Jae Jong , Lee Kangwon , Kim Tae-Don , Park Su A 
Published: January 2023

Abstract
Background: Patients face a serious threat if a solid tumor leaves behind partial residuals or cannot be completely removed after surgical resection. Immunotherapy has attracted attention as a method to prevent this condition. However, the conventional immunotherapy method targeting solid tumors, that is, intravenous injection, has limitations in homing in on the tumor and in vivo expansion and has not shown effective clinical results.
Method: To overcome these limitations, NK cells (Natural killer cells) were encapsulated in micro/macropore-forming hydrogels using 3D bioprinting to target solid tumors. Sodium alginate and gelatin were used to prepare micro-macroporous hydrogels. The gelatin contained in the alginate hydrogel was removed because of the thermal sensitivity of the gelatin, which can generate interconnected micropores where the gelatin was released. Therefore, macropores can be formed through bioprinting and micropores can be formed using thermally sensitive gelatin to make macroporous hydrogels.
Results: It was confirmed that intentionally formed micropores could help NK cells to aggregate easily, which enhances cell viability, lysis activity, and cytokine release. Macropores can be formed using 3D bioprinting, which enables NK cells to receive the essential elements. We also characterized the functionality of NK 92 and zEGFR-CAR-NK cells in the pore-forming hydrogel. The antitumor effects on leukemia and solid tumors were investigated using an in vitro model.
Conclusion: We demonstrated that the hydrogel encapsulating NK cells created an appropriate micro-macro environment for clinical applications of NK cell therapy for both leukemia and solid tumors via 3D bioprinting. 3D bioprinting makes macro-scale clinical applications possible, and the automatic process shows potential for development as an off-the-shelf immunotherapy product. This immunotherapy system could provide a clinical option for preventing tumor relapse and metastasis after tumor resection.
A comparative study of passive drug diffusion through human skin via intercellular and sweat duct route: effect of aging
Authors: Ranjan Aditya , Duryodhan Vijay S. , Patil Nagesh D. 
Published: January 2023

Abstract

A method of drug delivery that could provide control over
medicine reaching the bloodstream for systemic circulation would be
of immense importance. This work presents a comparative study of
the temporal and spatial variation of drugs diffusing passively
through two separate routes of human skin, namely intercellular
(ICR) and sweat duct route (SDR). Analysis is carried out for two
age groups (young < 40 years and old > 60 years of age).
Governing equations based on Fick’s law for mass transfer has been
solved numerically using an in-house developed code. The code has
been validated thoroughly with numerical and experimental work from
literature. Each skin route is modeled into three compartments
sandwiched between donor and receiver compartment. To understand
the role of diffusion and partition coefficient on drug permeation,
four drugs, namely hydrocortisone, transcinnamic acid, caffeine,
and benzoic acid, are considered. Drug diffusion rate is found
greater through ICR as compared to SDR. Further, the amount of
drugs diffusing through both routes increases with age. Desirable
drug characteristic is inferred to be a lower value of partition
coefficient and a higher value of diffusion coefficient. This study
could lead to real-time assessment of medicines reaching the
bloodstream and beyond.
Comparative effectiveness of Cangrelor in patients with acute coronary syndrome undergoing percutaneous coronary intervention: Insights from the multicenter M.O.Ca. registry
Authors: Pepe Martino , Carulli Eugenio , Larosa Claudio , Napoli Gianluigi , Nestola Palma Luisa , Carella Maria Cristina , Giordano Salvatore , Tritto Rocco Pio , Bartolomucci Francesco , Cirillo Plinio , Zoccai Giuseppe Biondi , Giordano Arturo , Ciccone Marco Matteo 
Published: January 2023

Abstract
Background: Cangrelor, the first intravenous P2Y12 inhibitor (P2Y12-I), has been approved on the basis of three large RCTs from the CHAMPION program which nevertheless have been criticized for the low bleeding risk of the enrolled patients, the large quote of chronic coronary syndromes (CCS), and the use of Clopidogrel as control arm even in the setting of acute coronary syndromes (ACS). We sought to investigate, in the setting of ACS, the comparative performance of Cangrelor in terms of in-hospital ischemic and haemorrhagic outcomes compared with the current gold-standard of oral P2Y12-I.
Methods: The study retrospectively enrolled 686 consecutive patients admitted to the Divisions of Cardiology of Policlinico of Bari and L. Bonomo Hospital of Andria for ACS and treated with percutaneous coronary intervention (PCI). The study population was divided according to the P2Y12-I treatment strategy in two groups: patients given an oral P2Y12-I and patients receiving Cangrelor in the CathLab followed by an oral P2Y12-I. Clinical endpoints included death, ischemic and bleeding events occurring during hospital stay.
Results: Cangrelor treated patients presented higher clinical risk profile at presentation and faced higher death rate. However, after PS matching, in-hospital mortality resulted comparable between the groups and Cangrelor use was associated with reduced in-hospital definite stent thrombosis (p=0.03).
Conclusions: Data from our real-world registry highlight that, in the setting of ACS, Cangrelor is prevalently used in patients with very challenging clinical presentations. The adjusted analysis provides for the first time promising data on stent thrombosis reduction associated with Cangrelor use.
Guided tissue regeneration techniques involving blood-derived products in periradicular surgery: a systematic review and meta-analysis
Authors: Baniulyte Gabriele , Burns Lorna , Ali Kamran 
Published: January 2023

Abstract
Background: Guided tissue regeneration (GTR) has been gaining popularity in dentistry as an aid to surgical techniques since 1982. The latest advancement in the GTR discipline examines the use of blood-derived products, but no systematic review had been carried out to date on its use in periradicular surgery. Therefore, the objective of this review was to evaluate the clinical outcomes of standard periradicular surgery versus periradicular surgery employing GTR techniques involving blood-derived products.
Methods: Randomised controlled trials investigating the outcomes of GTR techniques involving blood-derived products versus standard periradicular surgery technique, were included for review. Studies were excluded if they contain patients who have previously undergone periradicular surgery or treatment was carried out on unrestorable teeth (i.e., due to periodontal disease or root fractures). The databases MEDLINE, Embase, Dentistry and Oral Sciences Source, and Cochrane CENTRAL were used to locate published reports of studies, last searched on 16th December 2022. Reference lists of relevant past systematic reviews were used to identify further studies. Unpublished studies were sought using international trials registries and repositories. Two blinded reviewers carried out independent screening of records for inclusion and the selected studies were critically appraised using Joanna Briggs Institute critical appraisal tool prior to data extraction and synthesis. The results are presented in accordance with PRISMA guidelines.
Results: Two-hundred and sixty-one records were screened on title and abstract, and 17 studies were further screened against full text. Fourteen studies were excluded at this stage and 3 randomised controlled trials were included in the systematic review with a total of 85 patients. Meta-analysis was undertaken for the outcome of healing. The overall treatment effect was 0.78 (95% CI 0.18 to 3.34), favouring control group.
Discussion: The meta-analysis of 3 studies showed no significant difference in healing between intervention and control groups. Critical appraisal of the included trials identified deficiencies in indirectness and imprecision, downgrading the overall certainty to ‘low’. Therefore, more strong evidence is required to recommend routine use blood derived products to improve the clinical outcomes of periradicular surgery in conjunction with guided tissue regeneration.
Apigenin-Melatonin: a novel prostate cancer Radiation Sensitizer
Authors: Bahreghani Morteza Taghavi , Alizadeh Shaban , Geraily Ghazale , Shojaei Mehdi , Shirazi Alireza , rezapoor Saeed 
Published: January 2023

Abstract
Background: Radiotherapy is a common modality in prostate cancer (PrCa), with a dose of 80Gy. A higher dose of ionizing radiation is often needed to achieve an appropriate response; however, clinical evidence indicates that increasing the dose may lead to side effects. Therefore, finding a substance that sensitizes PrCa to ionizing radiation improves radiotherapy’s therapeutic efficacy. Thus, we aimed to assess the radiosensitization effect of Apigenin-Melatonin on Lymph Node Carcinoma of the Prostate (LNCaP).
Method: The effect of Apigenin, Melatonin, and ionizing radiation, alone and together, on the cells' viability plus the apoptosis induction, were tested by MTT assay, Annexin V, and PI. In addition, Bax and Bcl-2 genes expression was assessed by real-time PCR.
Results: LNCaP co-treatment by Apigenin and Melatonin inhibited PrCa cells' viability synergically. Also, LNCaP co-treatment with Apigenin and Melatonin, 2h before radiotherapy, significantly reduced the LNCaP cells' viability compared to the cells co-treated by Apigenin and Melatonin. Also, LNCaP co-treatment with Apigenin, Melatonin, and ionizing radiation significantly increased early apoptosis by decreasing Bcl-2 gene expression and increasing Bax gene expression.
Discussion: Our results revealed that Apigenin-Melatonin reduces LNCaP cells' viability. Plus, Apigenin-Melatonin could overcome the radioresistance of the LNCaP cells, enhancing radiation-induced apoptosis.
A poly(carbazole-alt-triazole) with thiabendazole side groups as an “on-off-on” fluorescent probe for detection of Cu(Ⅱ) ion and cysteine
Authors: Gao Liting , Xu Jinyao , Luo Haoyue , Lei Hong , Chen Xinai , Wan Jiayi , Feng Juhua , Liu Kuan 
Published: January 2023

Abstract
A novel conjugated polymer PCZBTA-TBZ containing thiabendazole as recognition unit was synthesized via Suzuki coupling reaction, and its structural characterization, spectroscopic analysis and photophysical properties were investigated. In the metal ion response study, the addition of Cu2+ led to the occurrence of the PET mechanism, which significantly quenched the fluorescence of the polymer PCZBTA-TBZ with a quenching effect of 98%. According to the density functional theory calculation, compared with other polycarbazoles or other alternative copolymers containing carbazole, with alternating carbazole and triazole enhances the electron mobility and reduces the energy band gap of the polymer. Due to the strong coordination ability between Cu2+ and Cys, the adding Cys competes the Cu2+ in the [PCZBTA-TBZ-Cu2+] complex, blocking the occurrence of PET, and the fluorescence intensity of PCZBTA-TBZ is restored. The addition of other amino acids caused almost no change. The polymer is expected to be used for dual fluorescence detection of specific metal ions and Cys.
Community Engagement Processes in Low- and Middle-Income Countries Health Research Settings: A Systematic Review of the Literature
Authors: Mthembu Zinhle , Mogaka John J O , Chimbari Moses J. 
Published: January 2023

AbstractBackground:Community Engagement is an important ethical imperative in research. Although substantial research emphasizes its real value and strategic importance, much of the available literature focuses primarily on the success of community participation, with little emphasis given to specific community engagement processes and strategies in relation to intended outcomes in research environments. The study’s objective was to explore the nature of community engagement strategies in health research settings in low- and middle-income countries.Methods:The study design was informed by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. We searched for peer-reviewed, English-language literature published between January 2011 and December 2021 through three databases on the internet (PubMed, Web of Science and Google Scholar). The terms "community engagement," "community involvement," "participation," "research settings," and "low- and middle-income countries" were merged in the search.Results:The majority of publications (8/10) were led by authors from low- and middle-income countries, with many of them (9/10) failing to continuously include important aspects of study quality. Articles were most likely to describe community engagement in consultation and information sessions, even though they were less participatory. The articles covered a wide range of health issues, but the majority were concerned with infectious diseases such as Malaria, HIV, and tuberculosis, followed by studies on the environment and broader health factors. Articles were largely under-theorized.Conclusions:Despite the lack of theoretical underpinnings for various community engagement strategies, community engagement in research settings was variable. Future studies should go deeper into community engagement theory, acknowledge the power dynamics underpin community engagement, and be more practical about the extent to which communities may participate.
Ready, Set, Go! The role of organizational readiness to predict adoption of a family caregiver training program using the Rogers’ Diffusion of Innovation Theory
Authors: Houtven Courtney H. Van , Drake Connor , Malo Teri L. , Decosimo Kasey , Tucker Matthew , Sullivan Caitlin , D’Adolf Josh , Hughes Jaime M. , Christensen Leah , Grubber Janet M. , Coffman Cynthia J. , Sperber Nina R. , Wang Virginia , Allen Kelli D. , Hastings S. Nicole , Shea Christopher M. , Zullig Leah L. 
Published: January 2023

Abstract
Background: Caregivers FIRST is an evidence-based program addressing gaps in caregivers’ skills. In 2020, the Veterans Health Administration Caregiver Support Program (CSP) Office nationally endorsed Caregivers FIRST, offering credit in leadership performance plans to encourage all 142 VA medical centers (VAMCs) to implement locally. Performance plan inclusion may create incentives to adopt but may be insufficient for early program implementation. Instead, organizational readiness for change, a theory-driven determinant of implementation, may be an important predictor of implementation.
Methods: In a cohort observational study, we surveyed CSP managers about their facilities’ readiness to implement using the Organizational Readiness for Implementing Change (ORIC) instrument. We compared ORIC change commitment (5 items) and change efficacy (7 items) domains among VAMCs that implemented Caregivers FIRST within one year (‘adopters’) to those that did not (‘non-adopters’). We categorized adopters from earliest to latest, as: ‘innovators’, ‘early adopters’, ‘early majority’ and ‘late adopters’ or ‘laggards’. We examined differences in ORIC domains and site characteristics between ‘adopters’, ‘non-adopters’, and between ‘adopter’ subcategories. We fit separate logistic regression models to assess whether ORIC and site characteristics were associated with early adoption among ‘adopters’.
Results: Fifty-one of 63 (81%) VAMCs with CSP manager survey respondents adopted Caregivers FIRST during the first year. ORIC change commitment and efficacy were similar for ‘adopters’ and ‘non-adopters’. However, sites that adopted earlier (innovators and early adopters) had higher ORIC change commitment and efficacy scores than the rest of the ‘adopters'. Logistic regression results indicated that higher ORIC change commitment (odds ratio [OR]= 2.57; 95% confidence interval [CI], 1.11–5.95)] and ORIC change efficacy (OR= 2.60; 95% CI, 1.12–6.03) scores were associated with increased odds that a VAMC was categorized as an ‘innovator’, ‘early adopter’, or ‘early majority’. Site level characteristics were not associated with Caregivers FIRST early adoption category.
Conclusions: To our knowledge, this study is the first to prospectively assess organizational readiness and the timing of subsequent program adoption. Early adoption was associated with higher ORIC change commitment and change efficacy and not site level characteristics. These findings yield insights into the role of organizational readiness to accelerate program adoption.
Trial registration: This study was registered on March 22, 2018 at ClinicalTrials.gov (identifier NCT03474380).
Development of a lightweight thermoelectric generator taking advantage of additive manufacturing technologies
Authors: Fanciulli Carlo , Abedi Hossein , Nespoli Adelaide , Dondè Roberto , Terra Caterina La , Migliorini Francesca , Passaretti Francesca , De Iuliis Silvana 
Published: January 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Clinical retrospective analysis with a predictive model for diffused-tenosynovial giant cell tumors of the temporomandibular joint
Authors: Liu Ying , Yingying Huang , Dongwang Zhu , Jiang Li , Tongchao Zhao , Yining He , Ronghui Xia , laiping Zhong 
Published: January 2023

Abstract
Background
This study aimed to find out the characteristics in relation to tumor recurrence in diffused-tenosynovial giant cell tumor of temporomandibular joint and to develop and validate the prognostic model for personalized prediction.
Methods
From April 2009 to January 2021, patients with diffused-tenosynovial giant cell tumor of temporomandibular joint at a single center were included in this study. The clinical features and local recurrence-free survival were assessed through the expression of the Ki-67 index and colony-stimulating factor 1 receptor expression. Both univariate and multivariate analyses were performed on the prognostic factors for local recurrence-free survival. An independent predictor nomogram and pertinent tumor characteristics were included.
Results
The retrospective study enrolling seventy eligible patients at the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine. During the follow-up time, eleven patients suffered tumor recurrence. Age was an independent risk factor for local recurrence-free survival (P = 0.032). The Ki-67 index varied significantly in different sites (P = 0.034) and tumor volume (P = 0.017). Multivariate logistic regression was used to develop the prediction model using both statistical significance and prognostic indicators. The C-index of the nomogram based on age, site, Ki-67, and colony-stimulating factor 1 receptor was 0.833. These variates provided good predicted accuracy for a nomogram on local recurrence-free survival. Diffused-tenosynovial giant cell tumor from the temporomandibular joint is extremely uncommon, and certain clinical traits are linked to the tumor proliferation index.
Conclusions
We identified the risk indicators and developed a nomogram in this study to forecast the likelihood of local recurrence-free survival in patients with diffused-tenosynovial giant cell tumor from temporomandibular joint.
Information Transparency with Immediate Release: Oncology Clinician and Patient Perceptions
Authors: O'Brien Elizabeth , Vemuru Sudheer , Leonard Laura , Himelhoch Ben , Taft Nancy , Jaiswal Kshama , Cumbler Ethan , Sams Sharon , Wolverton Dulcy , Ahrendt Gretchen , Yi Jeniann , Lin Chen-Tan , Miles Randy , Hampanda Karen , Tevis Sarah 
Published: January 2023

Abstract
Introduction:
As part of the 21st Century Cures Act, beginning April 2021, electronic health information (EHI) must be immediately released to patients. This change poses challenges for both oncology clinicians and patients whose priorities and experiences may differ. In this study, we sought to evaluate clinician and patient perspectives regarding the immediate release of EHI.
Methods:
Semi-structured interviews were conducted with a group of 8 clinicians and 12 patients using purposeful sampling to capture a diverse array of participants. Open-ended questions explored clinicians’ and patients’ perceptions of the immediate release of patient health information and how they adjusted to this change. Interviews were recorded, transcribed verbatim, and de-identified. Transcripts were thematically analyzed, reviewed to develop a coding rubric, and independently coded until thematic saturation was achieved.
Results:
Forty unique codes were identified, which were then mapped to 10 themes. These themes included Interpreting Results, Strategies for Patient Interaction, Patient Experiences, Communication Strategies, and Provider Limitations, among others. Clinicians and patients demonstrated varying levels of confidence in patient understanding of results and differences in perceived patient distress emphasizing the impersonal nature of electronic release and necessity for therapeutic clinician-patient communication. Unique recommendations to support patients receiving complex results reports were found.
Conclusions:
Clinicians and patients have varying insights on the role of immediate release of medical information in cancer care. Understanding these perspectives may be helpful for devising ways to improve communication between clinicians and patients and in developing patient-centered tools (glossaries, summary pages, additional resources, and more) to aid in understanding complex medical information.
Increased Risk of Death Following Recurrent Ketoacidosis Admissions: A Brazilian Cohort Study of Young Adults with Type 1 Diabetes
Authors: Santos Sarah S. , Ramaldes Luana A. L. , Dualib Patricia M. , Gabbay Monica A. L. , Sá João R. , Dib Sergio A. 
Published: January 2023

Abstract
Background: Recurrent DKA (rDKA) remains an acute type 1 diabetes complication even in post-insulin era. This study aimed to analyze the predictors and effects of rDKA on the mortality of patients with type 1 diabetes.
Methods: Patients hospitalized (n = 231) wih diabetic ketoacidosis (between 2007 to 2018) were included. Laboratorial and clinical variables were collected. Mortality curves were compared in four groups: diabetic ketoacidosis as a new-onset type 1 diabetes (group A), single diabetic ketoacidosis episode after diagnosis of type 1 diabetes (group B), 2–5 diabetic ketoacidosis events (group C), and >5 diabetic ketoacidosis events during follow-up period (group D).
Results: During the follow-up period (approximately 1823 days), the mortality rate was 16.02% (37/231). The median age at death was 38.7 years. In the survival curve analysis, at 1926 days (5 years), the probabilities of death were indicated by ratios of 7.78%, 4.58%, 24.40%, and 26.63% in groups A, B, C, and D, respectively. One diabetic ketoacidosis episode compared with ≥2 events had a relative risk of 4.49 (p = 0.004) of death. Neuropathy (relative risk 10.04; p < 0.001), retinopathy (relative risk 7.94; p < 0.01), nephropathy (relative risk 7.10; p < 0.001), mood disorders (relative risk 3.57; p = 0.002), antidepressant use (relative risk 3.09; p = 0.004), and statin use (relative risk 2.81; p = 0.0024) increased the risk of death.
Conclusions: Patients with type 1 diabetes with >2 diabetic ketoacidosis episodes have four times greater risk of death in 5 years. Microangiopathies, mood disorders, and use of antidepressants and statins were important risk factors for short-term mortality.