bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation, characterization and identification of cellulase (Endo-β-1,4-glucanase) producing bacteria from diverse locations
Authors: Kularajany Niranjan  , Kapilan Ranganathan  , Neelamanie Yapa  
Published: June 2023

The objective of the study was to isolate cellulase producing bacteria from natural sources and identify the bacterial isolates that produce thermostable enzyme. Bacteria were isolated from samples collected from different locations like mango leaf litter, termite gut, composting yard (municipal solid waste, compost, and soil), soil from decaying paper waste, decaying wood, and saline coastal belt soil by enrichment in broth containing 1% cellulose and subsequent culturing on plates and the purified cultures stored in slants of the solidified agar medium of the same composition as enrichment medium. Primary screening of the isolates for cellulase (Endo β-1,4-glucanase) production was done by growing them on Carboxy Methyl Cellulose (CMC) agar medium and screening using indicator dye Congo red. Secondary screening of the positive isolates showing cellulolytic Index > 4 was done by crude cellulase enzyme extraction and assay using 1% CMC in 1N citrate buffer (pH 5.0) at different pH values (4.8- 7.0) and temperatures (35- 70℃). Cellulase activity (EA) in U/mL was calculated based on the absorbance change per min. which depends on the amount of reducing sugar produced by the enzyme catalysed conversion of CMC to reducing sugar. Isolates were characterized morphologically and biochemically and six isolates (Gram -ve: T1, MSW, C1, S2 and Gram +ve: W2, P) having significantly higher EA were selected for DNA extraction. DNA was quantified and the DNA of four isolates (MSW, S2, W2, P) were selected for sequence analysis. The 16S rDNA sequencing was carried out through PCR amplification and Blast using NCBI blast similarity search tool resulted in the identification of MSW, S2, W2 as E.coli, Sulfitobacter pontiacus, Bacillus subtilis respectively and P as Bacillus cereus and Rhodococcus erythropolis strain e1. Isolates showed significantly higher cellulase activity (EA) at pH 6.2 and at varying temperatures. Bacterial isolates P and C2 showed significantly higher activity at 40℃, MSW at 60℃ and others at 50℃ (EA ranging from 0.0054 – 0.0604 U/mL). Isolates W2, P, S2 had EA ranging from 0.006 - 0.03 U/mL at 70℃. This study showed that the cellulase producers could be grown at different temperatures (40 - 70℃) on cellulose based substrate. In this study the substrate specificity indicates that the crude enzyme is an endo- β-1,4-glucanase that plays a prominent role in initiating and sustaining the hydrolytic process and randomly cleaves cellulose into glucose and olygomeric polysaccharides. Hence by selecting samples with cellulosic substance from different locations with extreme environmental conditions like high temperature, high salinity it is possible to isolate cellulolytic bacteria producing cellulase enzymes that are stable at higher processing temperatures and other harsh conditions which is of importance in industrial applications.
Keywords: Endo-β-1,4-glucanase, Cellulolytic index, Cellulase activity, Thermostable
Business and Adoption of Additive Manufacturing
Authors: Rogers Kirk , Barnes John 
Published: June 2023

Abstract
This article reviews business cases for additive manufacturing (AM) and offers suggestions on monetizing the flexibility created by AM through a deep understanding of the most applicable cost drivers. It also reviews the common adoption drivers for AM and provides suggestions on how to take advantage of them. The AM maturity model breaks down potential additively manufactured products into five levels: preproduction, production influence, substitution, functional designs, and multifunctional. The business value of these levels is further described and evaluated with respect to the triple constraint of project management. The article then focuses on success factors for implementing AM.
ormation of the Antimensia Collection at the Museum of the Moscow Theological Academy « Church-Archaeological Cabinet » and the History of Its Study
Authors: Чеботарева К. 
Published: June 2023

Статья посвящена антиминсной коллекции Музея МДА «ЦАК». Автор на основе архивных и библиотечных материалов рассказывает о том, как формировалась коллекция, называет имена дарителей, даёт обзор антиминсных выставок, проходивших в стенах Академии и за её пределами, приводит краткое описание антиминсов в современной экспозиции. В статье указаны авторы, чьи работы так или иначе были связаны с антиминсной коллекцией Музея МДА «ЦАК» или её отдельными экземплярами.
The article is devoted to the antimensia collection at the Museum of the Moscow Theological Academy «Church-Archaeological Cabinet». Using archival and library data, the author presents some key periods in the formation of the collection, reveals the contributors’ names, gives an overview of the antimensia stored at the Museum and briefly describes some currently exhibited items. The article also lists the authors whose works were associated with the antimensia collection at the Museum «Church-Archaeological Cabinet» or its individual items.
Perbandingan Penerapan Algoritma Dynamic Programming dengan Algoritma Greedy dalam Menentukan Optimasi Posisi Pasar di Suatu Wilayah
Authors: Thariq Ahmad 
Published: June 2023

Ketepatan penempatan lokasi pasar merupakan hal yang sangat penting bagi masyarakat di suatu wilayah. Banyaknya faktor yang mempengaruhi menuntut pemerintah harus bijak dalam mengambil keputusan. Salah satu yang paling berpengaruhi adalah dengan memperhitungkan kepadatan penduduk dan jarak antar wilayahnya. Oleh karena itu pada penelitian ini dilakukan penentuan lokasi pasar dengan menggunakan Algoritma Dynamic Programming yang dibandingkan dengan Algoritma Greedy sehingga penempatan pasar dapat dibuat seoptimal mungkin. Formulasi yang digunakan dalam penelitian ini menggunakan teori gaya interaksi antar dua muatan, dimana kepadatan penduduk sebagai muatan dan jarak wilayah sebagai pengganti jarak muatan. Dari hasil penelitian terlihat bahwa penempatan lokasi pasar berada di wilayah yang memiliki hubungan interaksi yang besar.
Kata kunci: Algoritma Dynamic, Algoritma Greedy, Pasar
Geological structure and occurrence conditions of the Bazhenov formation within THE South Tambey gas condensate field
Authors: Rasskazov A. A. , Potemkin G. N. 
Published: June 2023

Background. In the 21st century, exploration works in the previously discovered Jurassic deposits has been launched in the north of the Yamal Peninsula. The South Tambey field was no exception, where 3D seismic surveys and exploration drilling have been carried out over the past 10 years to study of Jurassic sediments and rocks of the Bazhenov formation. Due to the lack of a data sufficient sample in the northern regions of the Western Siberia, formation was poorly studied. The main ideas were formed on wells scattered at great distances from each other without the possibility of a clear correlation. After receiving new drilling materials, it became possible to examine the deposits of the Bazhenov formation in more detail.Aim. Determination of the structural features and occurrence conditions of the Bazhenov formation.Materials and methods. The research was based on the logging data of exploration and production drilling. A detailed correlation of well sections was performed, which was compared and analyzed with the core description and interpretation of seismic survey materials.Results. The lithological characteristics of rocks obtained by core description are presented, the atypical neighborhood of stromatolites and radiolarians in the section is noted. Based on a detailed correlation, the Bazhenov formation was divided into three intervals with characteristic geophysical logging values and distribution features over the area. The clinoform occurrence with development in the north-west direction is determined. Based on the areal variability, it is proposed to distinguish two types of the Bazhenov formation, and approaches to their tracing are considered.Conclusion. The study of the Bazhenov formation revealed a co-directionality of the spatial orientation of the faults, the progradation of the Achimov clinoforms, and changes in the thickness of the Bazhenov formation. A hypothesis has been put forward about a single tectonic regime that prevailed in the Upper Jurassic and Lower Cretaceous. It is proposed to consider flower (keyboard) tectonics as the main factor that determined the structure and occurrence conditions of the Bazhenov formation.
Petrophysical characteristics of carbonate rocks of organogenic structures of late devonian age (Voronezh horizon) in Orenburg region
Authors: Ryazanov A. R. 
Published: June 2023

Background. The carbonate rocks represented by organogenic structures of the Voronezh horizon (D3vr) at the Volostnovsky, Vostochno-Volostnovsky and Yuzhno-Volostnovsky fields in the northern part of the East-Orenburg oil and gas bearing region of the Volga-Ural oil and gas bearing province are studied.Aim. To establish a connection between the structures of lithological types of carbonate rocks and their reservoir properties within the organogenic structures of the Voronezh horizon at the Volostnovsky, Vostochno-Volostnovsky and Yuzhno-Volostnovsky deposits.Materials and methods. Stock materials on the geological structure of the northern part of the Vostochno-Orenburg district were used. Histograms of the distribution of porosity and permeability values were constructed. A petrophysical model was proposed followed by its verification and analysis.Results. Data on the filtration-capacitance properties of carbonate rocks were analyzed. Histograms reflecting changes in porosity and permeability for individual zones of organogenic structures were constructed. A petrophysical model describing the relationship between the lithological characteristics of rocks of the selected zones and their reservoir properties was proposed. The model was verified based on the data on the Volostnovsky site and neighboring deposits.Conclusion. The carbonate reservoirs of the Voronezh horizon are characterized by a complex structure with degraded reservoir properties. This information should be taken into account when developing deposits to ensure an increase in geological oil reserves and an extended service life of wells.
Management concepts for the sustainable development of mineral resources
Authors: Salmanov S. S. , Nazarova Z. M. , Leonidova Yu. A. 
Published: June 2023

Background. The development and implementation of the life cycle concepts of extracted raw materials seems promising for the sustainable development of mineral resources by a mining company. The practical implementation of such concepts requires a system of theoretical, methodological, analytical, and organizational procedures developed in close collaboration by state officials, researchers, and subsoil users with a particular focus on the responsibility of producers.Aim. To carry out an analysis of the existing theoretical concepts for managing the mineral resource base of mining companies and to assess their implementation prospects. The following objectives were formulated: 1) to analyze the current situation in the field of reproduction of mineral resources; 2) to review the management approaches currently used to reproduce mineral resources; 3) to evaluate the prospects of modern concepts for the management of mineral resources in the mining industry.Materials and methods of research. The authors analyzed the publications of foreign and domestic authors in the field of sustainable development. The complex of studies included an analysis of the theoretical concepts of management of reproduction of the mineral resource base developed to date, grouping of key indicators for assessing the sustainability of a mining enterprise, comparing approaches to management of reproduction of the mineral resource base and others.Results. Specific features of the mechanistic approach to the management of mineral resources, as well as its limitations and drawbacks, were analyzed. Particular attention was paid to a concept of the life cycle of a mining plant. The possibilities and prospects of using a methodology of mineral resource capacity management are described.Conclusions. The transition to modern concepts in the management of mining enterprises can be achieved under the condition of changing the existing orientation to super-profitable gross mining to that balancing social, environmental, and economic considerations.
EVALUASI PROGRAM PENDIDIKAN KARAKTER DI SMP ISLAM MATHOLI’UL FALAH BLORA PROVINSI JAWA TENGAH TAHUN 2021
Authors: Lelono Ragowo Sandhi 
Published: June 2023

Evaluation of the program to know the achievement of the program objectives by knowing the implementation of program activities. Context, Input, Process, Product (CIPP) evaluation model is an easier model to understand and assess in terms of character education in schools that will be thorough as a whole. This study uses a qualitative method with data collection techniques through interviews and documentation on school residents. The results showed that the character education program at Matholiuúl Falah Islamic Junior High School was almost entirely well run, the shortcomings occurred in the facilities and infrastructure, few students were not disciplined. The evaluation of the character education program at SMP Islam Matholi'ul Falah Blora, Central Java Province, has got good results in planting character values implemented, and internalization or habituation of character education of each student conducting activities in school. His advice is to improve the understanding and ability of school residents for character education in his school.
KUALITAS PELAYANAN PERUSAHAAN UMUM DAERAH AIR MINUM TIRTO NEGORO KABUPATEN SRAGEN
Authors: Haryanti Haryanti , Winarti Winarti , Pramono Joko 
Published: June 2023

Perusahaan Umum Daerah Air Minum Tirto Negoro adalah perusahaan pelayanan penyediaan dan penyaluran air minum kepada masyarakat yang merupakan Badan Usaha Milik Daerah (BUMD) Pemerintah Kabupaten Sragen, namun sejauh usianya saat ini yang telah mencapai 43 tahun masih terdapat permasalahan yang dihadapi di dalam memberikan pelayanan kepada masyarakat.
Penelitian ini bertujuan untuk mengetahui bagaimana kualitas pelayanan Perusahaan Umum Daerah Air Minum Tirto Negoro Kabupaten Sragen. Peneliti menggunakan pendekatan deskriptif kualitatif dan teknik purposive sampling. Teknik pengumpulan data melalui observasi, wawancara dan dokumentasi.
Adapun permasalahan yang terdapat pada Perumda Air Minum Tirto Negoro Kabupaten Sragen diantaranya adalah banyaknya pipa yang bocor karena usianya yang sudah tua sehingga kebocoran tersebut menyebabkan air kotor dan keruh, water meter yang rusak, debit air yang kecil saat hari Lebaran yang disebabkan karena pemakaian yang lebih banyak dari hari biasa serta kurang telitinya loket pembayaran PPOB (Payment Point Online Banking) yang menyebabkan kesalahan pembayaran dari pelanggan.
Hasil penelitian ini menunjukkan bahwa pelayanan yang diberikan oleh Perusahaan Umum Daerah Air Minum Tirto Negoro Kabupaten Sragen dari sisi tersedianya sarana dan prasarana serta fasilitas baik, kehandalan baik, jaminan baik, hanya dari sisi empati saja yang menjadi catatan untuk dilakukan peningkatan dalam melakukan pelayanan karena masih kurang baik.
Kata kunci: Kualitas, Pelayanan, Perusahaan Umum Daerah (Perumda) Air Minum, Tirto Negoro.
Socio-economic condition and indigenous poultry production scenario in a selected cluster area of Bangladesh
Authors: Shahjahan Md , Bhuiyan AKFH 
Published: June 2023

A baseline survey was conducted in a cluster area (279 households) of Mymensingh district in Bangladesh to know the farmers’ socio-economic condition, and overall production and husbandry practices regarding indigenous poultry farming and assess future outlook. Data were analyzed following descriptive statistics. Most of the farmers (54.84%) were in middle age type (31-50 years) and landless (41.58%). The average annual income of farmers recorded 61,260 BDT or 875 USD where agriculture (49.10%) was found as primary occupation. The average indigenous chicken (196 HHs), duck (16 HHs) and pigeon (4 HHs) distribution per household were 5.62, 3.81 and 3, respectively. Average egg production per clutch in chicken and duck identified 13.47 and 17.50 eggs, respectively, with 76.78 and 69.61% hatchability. Indigenous poultry (60.20%) housed inside the dwelling of farmers. Natural and uncontrolled breeding observed for all indigenous livestock in the studied area. Vaccination practice followed very limited for poultry while Newcastle disease found most prominent in poultry (65.82%). Proper initiative, funding, farm-based training to educated farmers including modern animal husbandry practices could increase indigenous poultry population and consequent profit.
Asian Australas. J. Biosci. Biotechnol. 2016, 1 (3), 557-563
ANALISIS KINERJA JARINGAN WIRELESS LAN MENGGUNAKAN METODE QOS DAN RMA
Authors: Sarah Astia Ningsih  , Subardin  , Gunawan  
Published: June 2023

Penelitian ini bertujuan untuk mengetahui hasil tentang kualitas kinerja jaringan Wireless LAN menggunakan metode QOS dan RMA di FMIPA UHO. Dalam penelitian ini digunakan lima parameter QOS yang terdiri dari throughput, packet loss, delay, jitter, dan MOS menggunakan software Wireshark. Metode RMA digunakan untuk melihat up time dan down time jaringan Wireless LAN menggunakan software PRTG.
Masalah yang cukup mengganggu kinerja dari jaringan Wireless LAN yang ada di Gazebo FMIPA UHO adalah masalah yang ditemukan yaitu sering terputusnya koneksi, hal ini disebabkan karena media perantara pada area ini menggunakan Wireless, sehingga jarak yang cukup jauh dan banyaknya user yang terhubung sangat mempengaruhi kinerja jaringan WLAN.
Berdasarkan hasil dari pembahasan analisis, diperoleh kualitas kinerja jaringan Wireless LAN Gazebo FMIPA UHO maka dapat disimpulkan sebagai berikut: 1) Parameter QoS yaitu throughput yang menurut versi TIPHON, Kapasitas bandwidth juga berpengaruh terhadap nilai QoS. Seperti yang telah diamati kecepatan transfer data nilai rata-rata tertinggi terjadi yaitu 11,870 Kbps. 2) Nilai delay rata rata tertinggi masih dibawah 150 ms yaitu 118.19 ms. 3) packet loss nilai rata rata packet loss termasuk dalam kategori bagus, karena nilai packet loss-nya 0,7 %. 4) Jitter kategori bagus dengan nilai rata- rata 19,4 ms dan pada jam siang dengan kondisi pada jam pagi tergolong pada kategori bagus yaitu rata- rata 25,81 ms.5) Nilai MOS berdasakan penilaian responden kualitas jaringan Gazebo FMIPA UHO yaitu 44 % untuk nilai tertinggi dengan kategori netral atau cukup baik. Hasil pengukuran Untuk nilai persentasi kemampuan jaringan Wi-Fi Gazebo FMIPA UHO untuk beroperasi tanpa terjadi kegagalan menggunakan metode RMA yaitu rata-rata waktu maximal adalah 3.69 % dan waktu minimal 2,71 %. Dari hasil pengolahan data QoS dan RMA yang telah dilakukan baik itu pada kondisi jam pagi dan pada kondisi jam siang dapat disimpulkan bahwa rata-rata kualitas jaringan internet yang ada tergolong dalam kategori cukup baik , ini artinya secara garis besar jaringan internet di Wi-Fi Gazebo FMIPA UHO yaitu cukup baik.
Assessing the influence of distinct culture media on human pre-implantation development using single-embryo transcriptomics
Authors: Ducreux Bastien , Barberet Julie , Guilleman Magali , Pérez-Palacios Raquel , Teissandier Aurélie , Bourc’his Déborah , Fauque Patricia 
Published: June 2023

The use of assisted reproductive technologies is consistently rising across the world. However, making an informed choice on which embryo culture medium should be preferred to ensure satisfactory pregnancy rates and the health of future children critically lacks scientific background. In particular, embryos within their first days of development are highly sensitive to their micro-environment, and it is unknown how their transcriptome adapts to different embryo culture compositions. Here, we determined the impact of culture media composition on gene expression in human pre-implantation embryos. By employing single-embryo RNA-sequencing after 2 or 5 days of the post-fertilization culture in different commercially available media (Ferticult, Global, and SSM), we revealed medium-specific differences in gene expression changes. Embryos cultured pre-compaction until day 2 in Ferticult or Global media notably displayed 266 differentially expressed genes, which were related to essential developmental pathways. Herein, 19 of them could have a key role in early development, based on their previously described dynamic expression changes across development. When embryos were cultured after day 2 in the same media considered more suitable because of its amino acid enrichment, 18 differentially expressed genes thought to be involved in the transition from early to later embryonic stages were identified. Overall, the differences were reduced at the blastocyst stage, highlighting the ability of embryos conceived in a suboptimal in vitro culture medium to mitigate the transcriptomic profile acquired under different pre-compaction environments.
High genetic risk for depression as an independent risk factor for mortality in patients referred for coronary angiography
Authors: Krämer Robert M. , Moissl Angela P. , Lorkowski Stefan , Krämer Bernhard K. , Lehtimäki Terho , Mishra Binisha H. , Mishra Pashupati P. , Leipe Jan , März Winfried , Kleber Marcus E. , Müller-Myhsok Bertram , Delgado Graciela E. 
Published: June 2023

BackgroundDifferent observations have suggested that patients with depression have a higher risk for a number of comorbidities and mortality. The underlying causes have not been fully understood yet.AimsThe aim of our study was to investigate the association of a genetic depression risk score (GDRS) with mortality [all-cause and cardiovascular (CV)] and markers of depression (including intake of antidepressants and a history of depression) in the Ludwigshafen Risk and Cardiovascular Health (LURIC) study involving 3,316 patients who had been referred for coronary angiography.Methods and resultsThe GDRS was calculated in 3,061 LURIC participants according to a previously published method and was found to be associated with all-cause (p = 0.016) and CV mortality (p = 0.0023). In Cox regression models adjusted for age, sex, body mass index, LDL-cholesterol, HDL-cholesterol, triglycerides, hypertension, smoking, and diabetes mellitus, the GDRS remained significantly associated with all-cause [1.18 (1.04–1.34, p = 0.013)] and CV [1.31 (1.11–1.55, p = 0.001)] mortality. The GDRS was not associated with the intake of antidepressants or a history of depression. However, this cohort of CV patients had not specifically been assessed for depression, leading to marked underreporting. We were unable to identify any specific biomarkers correlated with the GDRS in LURIC participants.ConclusionA genetic predisposition for depression estimated by a GDRS was independently associated with all-cause and CV mortality in our cohort of patients who had been referred for coronary angiography. No biomarker correlating with the GDRS could be identified.