bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustainable sourcing strategy for mega construction projects: An empirical analysis
Authors: Bagul Avinash , Balon Virendra , Mathur Arpita 
Published: May 2023

AbstractTime and cost overruns are the prominent issues faced by mega construction projects. To minimize cost overruns for the projects, a stepwise approach is suggested for the formulation of a sustainable sourcing strategy. Initially, weights are derived for the existing suppliers using the analytic hierarchy process to select the best set of suppliers. Multiple conflicting variables are considered for deriving the weights. Based on the derived weights, the supply network cost optimization model is developed for optimal order quantity allocation to the selected suppliers to maximize total purchasing value (TPV). A multi‐objective weighted goal programming model is used to minimize deviations in the TPV. Finally, the full factorial design of the experiment concept is employed to identify the significant factors impacting deviations in TPV. The methodology developed is verified using a case study approach. Mega‐construction project organization case was considered for analysis. Demand for the product, materials procurement cost, quality, and supplier lead time are found to be the significant factors impacting deviations in TPV. The developed approach can provide valuable insights to practitioners for the formulation of a sustainable sourcing strategy.
Characters of KRT80 and its roles in neoplasms diseases
Authors: Wei Xin‐Yuan , Zhao Jie , Tong Hao‐Bin , Cheng Shang‐Jie , He Na , Song Fei‐Xue 
Published: May 2023

AbstractBackgroundKRT80 is a human epithelial intermediate filament type II gene; its expression product is a component of intracellular intermediate filaments (IFs) and is involved in the assembly of the cytoskeleton. There is evidence that IFs form a dense network mainly in the perinuclear area, but they can also reach the cortex. They are essential for mechanical cushioning of cells, organelle positioning, cell apoptosis, migration, adhesion, and interactions with other cytoskeletal components. Humans possess 54 functional keratin genes, and KRT80 is one of the more unique genes. It is widely expressed in almost all epithelial cells, although it is structurally more similar to type II hair keratins than to type II epithelial keratins.AimIn this review, we summarize the basic facts about the keratin family and KRT80, the essential role of KRT80 in neoplasms, and its potential as a therapeutic target. We hope that this review will inspire researchers to at least partially focus on this area.ResultIn many neoplastic diseases, the high expression status of KRT80 and its role in regulating the biological functions of cancer cells have been well established. KRT80 can effectively enhance the proliferation, invasiveness and migration of cancer cells. However, the effects of KRT80 on prognosis and clinically relevant indices in patients with various cancers have not been extensively studied, and even opposite conclusions have been reached in different studies of the same cancer. Based on this, we should add more clinically relevant studies to clarify the prospect of clinical application of KRT80. Many researchers have made great progress in studying the mechanism of action of KRT80. However, their studies should be extended to more cancers to find common regulators and signaling pathways of KRT80 in different cancers. KRT80 may have far‐reaching effects on the human body, and this marker may play a crucial role in the function of cancer cells and the prognosis of cancer patients, so it has a promising future in the field of neoplasms.ConclusionIn neoplastic diseases, KRT80 is overexpressed in many cancers and plays an essential role in promoting proliferation, migration, invasiveness and poor prognosis. The mechanisms of KRT80 functions in cancer have been partially elucidated, suggesting that KRT80 is a potentially useful cancer therapeutic target. However, more systematic, in‐depth and comprehensive studies are still needed in this field.
Insulin‐like growth factor binding protein‐2 and glucose‐regulated protein 78 kDa: Potential biomarkers affect prognosis in IDH‐wildtype glioblastoma patients
Authors: Harland Abigail J. , Perks Claire M. , White Paul , Kurian Kathreena M. , Barber Hannah R. 
Published: May 2023

AbstractBackgroundThe overall survival of IDH‐wildtype glioblastoma patients is poor despite best available treatments. There is an urgent need for new biomarkers to inform more precise disease stratification. Previous studies have identified insulin‐like growth factor binding protein‐2 (IGFBP‐2) as a potential biomarker for glioblastoma diagnosis and therapeutic targeting. Other studies have indicated links between the insulin‐like growth factor (IGF) axis and tumorigenic functions of a molecular chaperone glucose related protein of 78 kDa (GRP78). We aimed to interrogate the oncogenic effects of IGFBP‐2 and GRP78 in our glioma stem cell (GSC) lines and clinical cohort.MethodsImmunoblotting, reverse transcription quantitative real‐time PCR were used to quantify protein and mRNA levels derived from GSCs and non‐malignant neural stem cells (NSCs). Microarray analysis was used to compare the differences in IGFBP‐2 (IGFBP‐2) and GRP78 (HSPA5) transcript expression between NSCs, GSCs and adult human cortex samples. Immunohistochemistry was used to quantify IGFBP‐2 and GRP78 expression in IDH‐wildtype glioblastoma tissue sections (n = 92) and clinical implications assessed using survival analysis. Finally, the relationship between IGFBP‐2 and GRP78 was further explored molecularly using coimmunoprecipitation.ResultsHere, we demonstrate that IGFBP‐2 and HSPA5 mRNA is overexpressed in GSCs and NSCs in comparison to non‐malignant brain tissue. We also determined a relationship in which G144 and G26 GSCs expressed higher IGFBP‐2 protein and mRNA than GRP78, and this was reversed in mRNA isolated from adult human cortex samples. Clinical cohort analysis revealed that Glioblastomas with high IGFBP‐2 protein expression paired with low GRP78 protein expression were significantly associated with a much shorter survival time (Median = 4 months, p = 0.019) compared with 12‐14 months for any other combination of high/low protein expression.ConclusionsInverse levels of IGFBP‐2 and GRP78 may be adverse clinical prognostic markers in IDH‐wildtype glioblastoma. Further interrogation of the mechanistic link between IGFBP‐2 and GRP78 may be important for rationalisation of their potential as biomarkers and therapeutic targets.
Immunotherapy or antiangiogenic therapy plus chemotherapy as first‐line treatment of patients with PD‐L1(‐) advanced non‐squamous non‐small cell lung cancer in a Chinese cohort
Authors: Xia Ruolan , Li Yanying , Yang Ling , Huang Meijuan 
Published: May 2023

AbstractPurposeFor patients with advanced nonsquamous non‐small cell lung cancer (NSCLC), immunotherapy or antiangiogenic therapy combined with pemetrexed and cisplatin/carboplatin have both shown significant efficacy at programmed cell death ligand 1 (PD‐L1) levels of <1%. Our study aimed to compare two first‐line regimens for patients with advanced nonsquamous NSCLC who were negative for PD‐L1.MethodsA retrospective cohort study was conducted comparing the outcomes of patients with advanced PD‐L1(‐) nonsquamous NSCLC who were treated with antiangiogenic therapy plus chemotherapy (A Group) to those who were treated with anti‐PD‐L1 monoclonal antibodies plus chemotherapy (mAbs) (B Group). Both regimens were evaluated for progression‐free survival (PFS), overall survival (OS), objective response rate (ORR), disease control rate (DCR), and side effects.Results114 patients were enrolled in the study, 82 in Group A and 32 in Group B. Those in Group A had a longer median PFS (9.8 vs. 6.7 months, p = 0.025). The OS was also achieved (p = 0.058). No statistically significant difference was seen in ORR (52.4% vs. 50.0%, p = 0.815) or DCR (93.9% vs. 87.5%, p = 0.225) between the two groups. Patients in the A group who did not smoke and did not have specific metastases could benefit from survival. Adverse events (AEs) in both groups were tolerated.ConclusionBevacizumab plus chemotherapy outperformed immunotherapy plus chemotherapy in terms of PFS.
Mycobacterium tuberculosis Rv1916 is an Acetyl‐CoA‐Binding Protein
Authors: Huang Evelyn Yu‐Wen , Kwai Brooke X. C. , Bhusal Ram Prasad , Bashiri Ghader , Leung Ivanhoe K. H. 
Published: May 2023

AbstractIsocitrate lyase (ICL) isoform 2 is an essential enzyme for some clinical Mycobacterium tuberculosis (Mtb) strains during infection. In the laboratory Mtb strain H37Rv, the icl2 gene encodes two distinct gene products – Rv1915 and Rv1916 – due to a frameshift mutation. This study aims to characterise these two gene products to understand their structure and function. While we were unable to produce Rv1915 recombinantly, soluble Rv1916 was obtained with sufficient yield for characterisation. Kinetic studies using UV‐visible spectrophotometry and 1H‐NMR spectroscopy showed that recombinant Rv1916 does not possess isocitrate lyase activity, while waterLOGSY binding experiments demonstrated that it could bind acetyl‐CoA. Finally, X‐ray crystallography revealed structural similarities between Rv1916 and the C‐terminal domain of ICL2. Considering the probable differences between full‐length ICL2 and the gene products Rv1915 and Rv1916, care must be taken when using Mtb H37Rv as a model organism to study central carbon metabolism.
Chemoenzymatic Oxidation of Diols Catalyzed by Co‐Immobilized Flavins and Dehydrogenases**
Authors: Castillo Pacheco Sergio F. , Moran Maria Jesus , Santos José I. , Salassa Luca , López‐Gallego Fernando 
Published: May 2023

AbstractEnzyme driven oxidations catalyzed by alcohol dehydrogenases rely on the in situ NAD(P)+ regeneration. A wide variety of chemoenzymatic and fully enzymatic methods have been reported over the last 30 years to integrate the cofactor regeneration in biocatalytic oxidations. However, the majority of examples are limited to homogeneous systems where the reuse of both enzymes and chemical catalysts are challenging. In this work, we co‐immobilize an alcohol dehydrogenase from Bacillus stearothermophilus with a flavin derivative (FMN), which performs as an organocatalyst that oxidizes NADH back to NAD+. This latter oxidized cofactor is sequentially utilized by the dehydrogenase to oxidize 1,ω‐diols. Remarkably, the immobilization chemistry of FMN determines its efficiency to oxidize NADH and, unlike in its free state, the immobilized FMN can recycle NAD+ in dark. This is possible because the support where both enzyme and FMN are immobilized also captures NADH, making the electron transfer from the substrates to the cofactors more efficient. This work illustrates how the co‐immobilization and confinement of bio and chemical catalysts on solid materials (heterogeneous phase) enable chemoenzymatic cascades that are precluded in solution (homogeneous phase).
Niobium‐doped Hematite Photoanodes Prepared through Low‐Cost Facile Methods for Photoelectrochemical Water Splitting
Authors: Melo Mauricio A. , Brito Igor M. , Mello João V. S. B. , Rocha Pamela S. M. , Bessa Isabela A. A. , Archanjo Braulio S. , Miranda Fabio S. , Cassella Ricardo J. , Ronconi Célia M. 
Published: May 2023

AbstractProducing solar fuels through semiconductor‐based photocatalytic water splitting is one of the main options for worldwide employment of non‐fossil energy sources. Nonetheless, the relatively low efficiency of the known photocatalysts, the high prices of composing elements, and the costly preparation methods render expensive devices that would not be economically competitive with fossil energy sources. Herein, we present a facile method for the preparation of both round hematite (Fe2O3) nanoparticles, with average diameter of (56±7) nm, and of niobium‐doped hematite nanoparticles with similar size and morphology. Thin films of these nanoparticles were assembled through electrophoretic deposition, resulting in homogeneous photoanodes with nanometer thickness that can split water, under simulated solar irradiation, to produce oxygen in a photoelectrochemical cell. The optimization of the film composition and thickness resulted in the improvement of the photocurrent from 352.3 to 1,342.5 μA cm−2 at 1.23 V vs RHE for the pristine hematite and Nb‐doped hematite, respectively, both with thickness of (499±63) nm. This enhancement in the oxidation reaction yield is a clear consequence of the improvement of charge carriers transport along the nanoparticle structure due to niobium doping and adequate light absorption/charge transfer balance of the photoelectrode under operation.
From Terminal to Spiro‐Phosphonium Acceptors, Remarkable Moieties to Develop Polyaromatic NIR Dyes
Authors: Partanen Iida , Belyaev Andrey , Su Bo‐Kang , Liu Zong‐Ying , Saarinen Jarkko J. , Ibni Hashim Ishfaq , Steffen Andreas , Chou Pi‐Tai , Romero‐Nieto Carlos , Koshevoy Igor O. 
Published: May 2023

AbstractPhosphonium‐based compounds gain attention as promising photofunctional materials. As a contribution to the emerging field, we present a series of donor‐acceptor ionic dyes, which were constructed by tailoring phosphonium (A) and extended π‐NR2 (D) fragments to an anthracene framework. The alteration of the π‐spacer of electron‐donating substituents in species with terminal −+PPh2Me groups exhibits a long absorption wavelength up to λabs=527 nm in dichloromethane and shifted the emission to the near‐infrared (NIR) region (λ=805 nm for thienyl aniline donor), although at low quantum yield (Φ<0.01). In turn, the introduction of a P‐heterocyclic acceptor substantially narrowed the optical bandgap and improved the efficiency of fluorescence. In particular, the phospha‐spiro moiety allowed to attain NIR emission (797 nm in dichloromethane) with fluorescence efficiency as high as Φ=0.12. The electron‐accepting property of the phospha‐spiro constituent outperformed that of the monocyclic and terminal phosphonium counterparts, illustrating a promising direction in the design of novel charge‐transfer chromophores.
RNA‐Catalyzed RNA Ligation within Prebiotically Plausible Model Protocells**
Authors: DasGupta Saurja , Zhang Stephanie J. , Smela Merrick P. , Szostak Jack W. 
Published: May 2023

AbstractDemonstrating RNA catalysis within prebiotically relevant models of primordial cells (protocells) remains a challenge in origins of life research. Fatty acid vesicles encapsulating genomic and catalytic RNAs (ribozymes) are attractive models for protocells; however, RNA catalysis has largely been incompatible with fatty acid vesicles due to their instability in the presence of Mg2+ at the concentrations required for ribozyme function. Here, we report a ribozyme that catalyzes template‐directed RNA ligation at low Mg2+ concentrations and thus remains active within stable vesicles. Ribose and adenine, both prebiotically relevant molecules, were found to greatly reduce Mg2+‐induced RNA leakage from vesicles. When we co‐encapsulated the ribozyme, substrate, and template within fatty acid vesicles, we observed efficient RNA‐catalyzed RNA ligation upon subsequent addition of Mg2+. Our work shows that RNA‐catalyzed RNA assembly can occur efficiently within prebiotically plausible fatty acid vesicles and represents a step toward the replication of primordial genomes within self‐replicating protocells.
Symmetry‐Broken Charge‐Transfer Excited State in Homoleptic Zinc(II) Imidazo[1,2‐a]pyridine Complexes
Authors: Giuso Valerio , Jouaiti Elise , Cebrián Cristina , Parant‐Aury Sabrina , Kyritsakas Nathalie , Gourlaouen Christophe , Mauro Matteo 
Published: May 2023

AbstractA series of four homoleptic cationic ZnII complexes (Zn1–Zn4) coordinated by two substituted bis‐imidazo[1,2‐a]pyridine (ImPy) ligands (L1–L4) is herein presented. The ligands are functionalized with either electron acceptor or donor functional groups at the C6 positions of the two ImPy moieties. In DMF solution, all of the complexes display intense near‐UV to blue emission (λem=379–450 nm) with high photoluminescence quantum yields (PLQY) up to 0.50 and short‐lived excited states. Additionally, complex Zn4, containing the ‐NPh2 functionalized ligand, displays an interesting solvatochromic behavior. The absorption spectrum is characterized by electronic transitions with mainly ligand‐centered (1LC) and intraligand charge‐transfer (1ILCT) character, which involves a symmetric electron density redistribution at Franck‐Condon (FC). In stark contrast, the subsequent excited‐state dynamics relaxes the molecular symmetry and allows symmetry‐breaking hole‐electron pair redistribution on the bichromophoric system. As a consequence, an efficient radiative de‐excitation process takes place that is ascribed to a singlet‐manifold excited state with symmetry‐broken charge transfer (1SBCT) character, as supported by an in‐depth photophysical, electrochemical and time‐dependent density functional theory (TD‐DFT) investigation. Remarkably, the 1SBCT nature provides structureless green photoluminescence in the solid state (λem up to 520 nm for Zn4), which is rather uncommon for ZnII complexes.
Dietary overlap and selectivity among mountain steppe river fish in the United States and Mongolia
Authors: Minder Mario , Arsenault Emily R. , Pyron Mark , Otgonganbat Amarbat , Mendsaikhan Bud 
Published: May 2023

AbstractLotic systems in mountain regions have historically provided secure habitat for native fish populations because of their relative isolation from human settlement and lack of upstream disturbances. However, rivers of mountain ecoregions are currently experiencing heightened levels of disturbance due to the introduction of nonnative species impacting endemic fishes in these areas. We compared the fish assemblages and diets of mountain steppe fishes of the stocked rivers in Wyoming with rivers in northern Mongolia where stocking is absent. Using gut content analysis, we quantified the selectivity and diets of fishes collected in these systems. Nonnative species had more generalist diets with lower levels of selectivity than most native species and native species had high levels of dietary specificity and selectivity. High abundances of nonnative species and high levels of dietary overlaps in our Wyoming sites is a cause of concern for native Cutthroat Trout and overall system stability. In contrast, fish assemblages characterizing Mongolia mountain steppe rivers were composed of only native species with diverse diets and higher selectivity values, suggesting low probability for interspecific competition.
Electronic nudges to increase influenza vaccination uptake among patients with heart failure: A pre‐specified analysis of the NUDGE‐FLU trial
Authors: Johansen Niklas Dyrby , Vaduganathan Muthiah , Bhatt Ankeet S. , Lee Simin Gharib , Modin Daniel , Claggett Brian L. , Dueger Erica L. , Samson Sandrine , Loiacono Matthew M. , Harris Rebecca C. , Køber Lars , Solomon Scott D. , Sivapalan Pradeesh , Jensen Jens Ulrik Stæhr , Martel Cyril Jean‐Marie , Valentiner‐Branth Palle , Krause Tyra Grove , Biering‐Sørensen Tor 
Published: May 2023

AbstractAimsSeasonal influenza vaccination is strongly recommended in patients with heart failure (HF). The NUDGE‐FLU trial recently found two electronic behavioural nudging letter strategies – a letter highlighting potential cardiovascular benefits of vaccination and a repeated letter at day 14 –effective in increasing influenza vaccination in Denmark. The aims of this pre‐specified analysis was to further examine vaccination patterns and effects of these behavioural nudges in patients with HF including potential off‐target effects on guideline‐directed medical therapy (GDMT) use.Methods and resultsThe nationwide NUDGE‐FLU trial randomized 964 870 Danish citizens ≥65 years to usual care or nine different electronic nudging letter strategies. Letters were delivered through the official Danish electronic letter system. The primary endpoint was the receipt of an influenza vaccine; additional outcomes for this analysis included GDMT use. In this analysis, we also assessed influenza vaccination rates in the overall Danish HF population including those <65 years (n = 65 075). During the 2022–2023 season, influenza vaccination uptake was 71.6% in the overall Danish HF population but this varied considerably with only 44.6% uptake in those <65 years. A total of 33 109 NUDGE‐FLU participants had HF at baseline. Vaccination uptake was higher among those on higher levels of baseline GDMT (≥3 classes: 85.3% vs. ≤2 classes: 81.9%; p < 0.001). HF status did not modify the effects of the two overall successful nudging strategies on influenza vaccination uptake (cardiovascular gain‐framed letter: pinteraction = 0.37; repeated letter: pinteraction = 0.55). No effect modification was observed across GDMT use levels for the repeated letter (pinteraction = 0.88), whereas a trend towards attenuated effect among those on low levels of GDMT was observed for the cardiovascular gain‐framed letter (pinteraction = 0.07). The letters had no impact on longitudinal GDMT use.ConclusionsApproximately one in four patients with HF did not receive influenza vaccination with a pronounced implementation gap in those <65 years where less than half were vaccinated. HF status did not modify the effectiveness of cardiovascular gain‐framed and repeated electronic nudging letters in increasing influenza vaccination rates. No unintended negative effects on longitudinal GDMT use were observed.Clinical Trial Registration: ClinicalTrials.gov NCT05542004.
Experimental Investigation of Pure Spinel Mn3O4 Properties Synthesized through Chemical Spray Pyrolysis for Future Gas Sensor Application
Authors: Doubi Youssef , Hartiti Bouchaib , Siadat Maryam , Labrim Hicham , Fadili Salah , Tahri Mounia , Stitou Mohamed , Thevenin Philippe , Losson Etienne 
Published: May 2023

AbstractThe semiconductor realization is a very significant stage in gas sensor application. Herein, the Mn3O4 semiconductor was deposited using chemical spray pyrolysis. The effect of deposition temperature on structural, vibrational optical and electrical Mn3O4 thin layers properties were investigated through: X‐ray diffraction, Raman spectroscopy, UV‐visible spectrophotometer, and two points electrometers respectively. The X‐ray diffraction showed the appearance of spinel phase of tetragonal Mn3O4 with strong formation direction along (101) plan and without any secondary phase indicating the formation of pure Mn3O4. The Raman spectroscopy confirmed the results obtained in XRD and certified the high‐quality formation of Mn3O4. In addition, the crystallinity improvement (the increase of crystallite size and the decrease of dislocation density) was caused by the increasing of deposition temperature from 350 °C to 450 °C. Optical properties such as transmittance, absorbance and band gap energy were extracted by UV‐Visible spectrophotometer. Thus, low transmittance, high absorbance and small band gap energy were observed at the highest substrate temperature (450 °C). The electrical conductivity showed good values between 4.83 and 13.89 mS.cm−1. These properties make Mn3O4 an appropriate material to be used as a sensitive layer in gas sensors applications.
Formal Addition of β‐Acylalkenyl Anions to Ketones Utilizing [1,2]‐Phospha‐Brook Rearrangement under Brønsted Base Catalysis
Authors: Kondoh Azusa , Takada Sena , Aita Kohei , Terada Masahiro 
Published: May 2023

AbstractA methodology for the formal addition of β‐acylalkenyl anions was developed by utilizing the [1,2]‐phospha‐Brook rearrangement under Brønsted base catalysis. The two‐step reaction involves the catalytic addition of α‐oxygenated propargyl anions generated via [1,2]‐phospha‐Brook rearrangement to electron‐deficient ketones and subsequent alcoholysis to afford tertiary alcohols having an enone moiety. This is a rare example of a catalytic carbon‐carbon bond forming reaction of β‐acylalkenyl anion equivalents, providing synthetic building blocks that are otherwise difficult to access.