bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neutrophil extracellular traps in autoimmune diseases: Analysis of the knowledge map
Authors: Wang Wei , Su Jing , Kang Wenjuan , Yan Meiqin , Pan Jie , Zhang Xianhui 
Published: January 2023

IntroductionRecent studies have shown much progress in the research of neutrophil extracellular traps (NETs) in autoimmune diseases (AIDs). However, there is no bibliometric analysis in this research field. This study aimed to provide a bibliometrics review of the knowledge structure and research hotspots of NETs in AIDs.MethodsArticles relevant to NETs in AIDs from 2010 to 2022 were retrieved through the Web of Science Core Collection (WoSCC) database. This bibliometric analysis was performed by VOSview, CiteSpace, and Scimago Graphica.ResultsA total of 289 papers analyzed in this research were from 493 organizations in 47 countries by 1537 authors. They were published in 133 journals and cited 20,180 citations from 2,465 journals. The number of annual publications in this field is growing steadily and rapidly, with the United States, China and Germany leading the research effort. Frontiers in Immunology and Journal of Immunology have significantly impacted research in this field. Kaplan, Mariana J, from the National Institutes of Health (The United States), has the most published articles, and Brinkmann, v, from Max Planck Institute for Infection Biology (Germany), is the most co-cited author. Systemic lupus erythematosus and rheumatoid arthritis are the leading topics in this field. The trend of clinical application in the future is the development of new therapies by controlling NETs in the progression of AIDs.ConclusionsOur study summarized the research trends and developments of NETs in AIDs in recent years and would provide a reference for scholars in this field.
Immunotherapy with immune checkpoint inhibitors and predictive biomarkers in malignant mesothelioma: Work still in progress
Authors: Perrino Matteo , De Vincenzo Fabio , Cordua Nadia , Borea Federica , Aliprandi Marta , Santoro Armando , Zucali Paolo Andrea 
Published: January 2023

Malignant mesothelioma (MM) is a rare and aggressive neoplasm, usually associated with a poor prognosis (5 years survival rate &lt;10%). For unresectable disease, platinum and pemetrexed chemotherapy has been the only standard of care in first line for more than two decades, while no standard treatments have been approved in subsequent lines. Recently, immunotherapy has revolutionized the therapeutic landscape of MM. In fact, the combination of ipilimumab plus nivolumab has been approved in first line setting. Moreover, immune checkpoint inhibitors (ICIs) showed promising results also in second-third line setting after platinum-based chemotherapy. Unfortunately, approximately 20% of patients are primary refractory to ICIs and there is an urgent need for reliable biomarkers to improve patient’s selection. Several biological and molecular features have been studied for this goal. In particular, histological subtype (recognized as prognostic factor for MM and predictive factor for chemotherapy response), programmed death ligand 1 (PD-L1) expression, and tumor mutational burden (widely hypothesized as predictive biomarkers for ICIs in several solid tumors) have been evaluated, but with unconclusive results. On the other hand, the deep analysis of tumor infiltrating microenvironment and the improvement in genomic profiling techniques has led to a better knowledge of several mechanisms underlying the MM biology and a greater or poorer immune activation. Consequentially, several potential biomarkers predictive of response to immunotherapy in patients with MM have been identified, also if all these elements need to be further investigated and prospectively validated.In this paper, the main evidences about clinical efficacy of ICIs in MM and the literature data about the most promising predictive biomarkers to immunotherapy are reviewed.
Assessment of the physiological vulnerability of the endemic and critically endangered Daggernose Shark: A comparative approach to other Carcharhiniformes
Authors: Wosnick Natascha , Chaves Ana Paula , Dias Héllida Negrão , Onodera Palmeira Nunes Ana Rita , Nunes Jorge Luiz Silva , Hauser-Davis Rachel Ann 
Published: January 2023

IntroductionThe current Isogomphodon oxyrhynchus (Daggernose Shark) population status Q7points to 99% losses in the last decade due to certain biological traits, site fidelity, and historical high representativeness as bycatch in artisanal fisheries. This species is listed as Critically Endangered (CR), both in the IUCN and the Brazilian Red Lists. Its vulnerability is so high that its recovery potential requires protection from ongoing fishing pressure.ObjectiveIn this context, this study aimed to evaluate the health status of Daggernose sharks and their ability to cope with allostatic overload in a comparative analysis with other Carcharhinid and Sphyrnid sharks.MethodsSharks incidentally caught by the artisanal fleet in the state of Maranhão, on the Brazilian Amazon Coast, were sampled for blood, and serum was used to assess biochemical markers.ResultsThe findings indicate significant differences in Daggernose Shark homeostatic capacity for ALP, ALT/GTP, creatinine, lactate, urea, total cholesterol, and triglycerides, pointing to lower health scores and recovery capacity when compared to other Carcharhiniformes inhabiting the same region.Discussion and conclusionsIt is possible that such vulnerability is a result of fisheries-induced evolution, leading to remaining populations with very low chances of fully recovering. Conservation planning is thus urgent, as current legislation based on fishing bans does very little for the species. International collaboration and longterm recovery measures are necessary, including the creation of MPAs specially designed for the species and captive maintenance aiming to monitor health status and carry out breeding attempts.
Effects of sequential inhibitory and facilitatory repetitive transcranial magnetic stimulation on neurological and functional recovery of a patient with chronic stroke: A case report and literature review
Authors: Chen Nan , Qiu Xiao , Hua Yan , Hu Jian , Bai Yulong 
Published: January 2023

Background and purposeThe effects of conventional protocols of repetitive transcranial magnetic stimulation (rTMS) in the chronic phase of stroke are limited. This study aimed to apply the sequential inhibitory and facilitatory rTMS for upper limb motor dysfunction post-stroke to observe the efficacy and explore the possible neurophysiological mechanism. We hypothesize that this protocol would both enhance the excitability of affected M1 and promote connections among motor areas.Case descriptionWe reported a 55-year-old female patient with a 1-year chronic stroke and right-sided hemiplegia, who underwent the 14-session rTMS with seven sessions of low frequency (LF) and with seven sessions of high frequency (HF). Clinical scales mainly including Fugl-Meyer Assessment of Upper Extremity (FMA-UE), Action Research Arm Test (ARAT), neurophysiological measures, and functional near-infrared spectroscopy (fNIRS) were assessed before (T0), at the midpoint (T1), and after the intervention (T2).OutcomesThe patient exhibited post-intervention improvement in upper extremity function. There was increased excitability in the ipsilesional hemisphere and the opposite in the contralesional hemisphere. The interhemispheric inhibition (IHI) ratio increased from 2.70 to 10.81 and finally decreased to 1.34. Oxy-Hb signal was significantly decreased in affected M1 and mildly decreased in unaffected M1, while that of PMC and SMA on the affected side increased significantly.ConclusionThe sequential inhibitory and facilitatory rTMS significantly promoted motor recovery in the patient. Related mechanisms include upregulation of excitability in the ipsilesional hemisphere, return of interhemispheric balance, and neuroplasticity-induced cortical reorganization.
Low-density lipoprotein balances T cell metabolism and enhances response to anti-PD-1 blockade in a HCT116 spheroid model
Authors: Babl Nathalie , Hofbauer Joshua , Matos Carina , Voll Florian , Menevse Ayse Nur , Rechenmacher Michael , Mair Ruth , Beckhove Philipp , Herr Wolfgang , Siska Peter J. , Renner Kathrin , Kreutz Marina , Schnell Annette 
Published: January 2023

IntroductionThe discovery of immune checkpoints and the development of their specific inhibitors was acclaimed as a major breakthrough in cancer therapy. However, only a limited patient cohort shows sufficient response to therapy. Hence, there is a need for identifying new checkpoints and predictive biomarkers with the objective of overcoming immune escape and resistance to treatment. Having been associated with both, treatment response and failure, LDL seems to be a double-edged sword in anti-PD1 immunotherapy. Being embedded into complex metabolic conditions, the impact of LDL on distinct immune cells has not been sufficiently addressed. Revealing the effects of LDL on T cell performance in tumor immunity may enable individual treatment adjustments in order to enhance the response to routinely administered immunotherapies in different patient populations. The object of this work was to investigate the effect of LDL on T cell activation and tumor immunity in-vitro. MethodsExperiments were performed with different LDL dosages (LDLlow = 50 μg/ml and LDLhigh = 200 μg/ml) referring to medium control. T cell phenotype, cytokines and metabolism were analyzed. The functional relevance of our findings was studied in a HCT116 spheroid model in the context of anti-PD-1 blockade.ResultsThe key points of our findings showed that LDLhigh skewed the CD4+ T cell subset into a central memory-like phenotype, enhanced the expression of the co-stimulatory marker CD154 (CD40L) and significantly reduced secretion of IL-10. The exhaustion markers PD-1 and LAG-3 were downregulated on both T cell subsets and phenotypical changes were associated with a balanced T cell metabolism, in particular with a significant decrease of reactive oxygen species (ROS). T cell transfer into a HCT116 spheroid model resulted in a significant reduction of the spheroid viability in presence of an anti-PD-1 antibody combined with LDLhigh.DiscussionFurther research needs to be conducted to fully understand the impact of LDL on T cells in tumor immunity and moreover, to also unravel LDL effects on other lymphocytes and myeloid cells for improving anti-PD-1 immunotherapy. The reason for improved response might be a resilient, less exhausted phenotype with balanced ROS levels.
Changes in cystic fibrosis transmembrane conductance regulator protein expression prior to and during elexacaftor-tezacaftor-ivacaftor therapy
Authors: Stanke Frauke , Pallenberg Sophia T. , Tamm Stephanie , Hedtfeld Silke , Eichhorn Ella M. , Minso Rebecca , Hansen Gesine , Welte Tobias , Sauer-Heilborn Annette , Ringshausen Felix C. , Junge Sibylle , Tümmler Burkhard , Dittrich Anna-Maria 
Published: January 2023

Background: Defects in expression, maturation or function of the epithelial membrane glycoprotein CFTR are causative for the progressive disease cystic fibrosis. Recently, molecular therapeutics that improve CFTR maturation and functional defects have been approved. We aimed to verify whether we could detect an improvement of CFTR protein expression and maturation by triple therapy with elexacaftor-tezacaftor-ivacaftor (ELX/TEZ/IVA).Methods: Rectal suction biopsies of 21 p.Phe508del homozygous or compound heterozygous CF patients obtained pre- and during treatment with ELX/TEZ/IVA were analyzed by CFTR Western blot that was optimized to distinguish CFTR glycoisoforms.Findings: CFTR western immunoblot analysis revealed that—compared to baseline—the levels of CFTR protein increased by at least twofold in eight out of 12 patients upon treatment with ELX/TEZ/IVA compared to baseline (p &lt; 0.02). However, polydispersity of the mutant CFTR protein was lower than that of the fully glycosylated wild type CFTR Golgi isoform, indicating an incompletely glycosylated p.Phe508el CFTR protein isoform C* in patients with CF which persists after ELX/TEZ/IVA treatment.Interpretation: Treatment with ELX/TEZ/IVA increased protein expression by facilitating the posttranslational processing of mutant CFTR but apparently did not succeed in generating the polydisperse spectrum of N-linked oligosaccharides that is characteristic for the wild type CFTR band C glycoisoform. Our results caution that the lower amounts or immature glycosylation of the C* glycoisoform observed in patients’ biomaterial might not translate to fully restored function of mutant CFTR necessary for long-term provision of clinical benefit.
H+-pyrophosphatases enhance low nitrogen stress tolerance in transgenic Arabidopsis and wheat by interacting with a receptor-like protein kinase
Authors: Zhang Huijuan , Chen Ming , Xu Chengjie , Liu Rongbang , Tang Wensi , Chen Kai , Zhou Yongbin , Xu Zhaoshi , Chen Jun , Ma Youzhi , Chen Weiguo , Sun Daizhen , Fan Hua 
Published: January 2023

IntroductionNitrogen is a major abiotic stress that affects plant productivity. Previous studies have shown that plant H+-pyrophosphatases (H+-PPases) enhance plant resistance to low nitrogen stress. However, the molecular mechanism underlying H+-PPase-mediated regulation of plant responses to low nitrogen stress is still unknown. In this study, we aimed to investigate the regulatory mechanism of AtAVP1 in response to low nitrogen stress.Methods and ResultsAtAVP1 in Arabidopsis thaliana and EdVP1 in Elymus dahuricus belong to the H+-PPase gene family. In this study, we found that AtAVP1 overexpression was more tolerant to low nitrogen stress than was wild type (WT), whereas the avp1-1 mutant was less tolerant to low nitrogen stress than WT. Plant height, root length, aboveground fresh and dry weights, and underground fresh and dry weights of EdVP1 overexpression wheat were considerably higher than those of SHI366 under low nitrogen treatment during the seedling stage. Two consecutive years of low nitrogen tolerance experiments in the field showed that grain yield and number of grains per spike of EdVP1 overexpression wheat were increased compared to those in SHI366, which indicated that EdVP1 conferred low nitrogen stress tolerance in the field. Furthermore, we screened interaction proteins in Arabidopsis; subcellular localization analysis demonstrated that AtAVP1 and Arabidopsis thaliana receptor-like protein kinase (AtRLK) were located on the plasma membrane. Yeast two-hybrid and luciferase complementary imaging assays showed that the AtRLK interacted with AtAVP1. Under low nitrogen stress, the Arabidopsis mutants rlk and avp1-1 had the same phenotypes.DiscussionThese results indicate that AtAVP1 regulates low nitrogen stress responses by interacting with AtRLK, which provides a novel insight into the regulatory pathway related to H+-pyrophosphatase function in plants.
Understanding employee creativity from the perspectives of grit, work engagement, person organization fit, and feedback
Authors: Gonlepa Miapeh Kous , Dilawar Sana , Amosun Tunde Simeon 
Published: January 2023

PurposeDrawing on the growing emphasis in the literature on the importance of creativity in the workplace, the present study examines the effect of personal and organizational level factors that influences employee creativity. Precisely, we examine how grit, work engagement, person-organization fit, and feedback influence creativity in the workplace.Design/methodology/approachWe sampled data from 422 research assistants who are professional workers at top-notch Universities in China. They were recruited to participate in the survey through an online medium known as WeChat. We empirically tested the effect of grit, work engagement, person-organization fit, and feedback on employee creativity. These hypotheses were supported by confirmatory and exploratory factor analysis, and path analysis.FindingsThe results show that work engagement strengthens the relationship between grit and creativity. In addition, the results proved that person-organization fit positively moderates the link between grit and work engagement. Feedback also had a positive mediating effect on the link between work engagement and creativity.Practical implicationsBased on the appropriateness of an individual work environment, a gritty person will likely become engaged and creative with task execution. Consequently, a person’s organizational fit will strongly interact with grit, work engagement, and creativity.Originality/valueWe shed light on the blended value of personal and organizational-level factors that positively affect creativity in the workplace. Specifically, grit being both a personal and organizational factor influences employee creativity via work engagement. This research explored the effect of work engagement as a mechanism that serves as a motivational feature enhancing creativity. We also simultaneously identify the moderation conditions of person-organization fit and feedback. The theoretical and practical implications of the findings are discussed in detail. The study makes a theoretical contribution through its assessment of the impact of grit on employee creativity. The trait activation theory portrays how grit can be expressed through feedback and person-organization fit. In terms of practice, grit can be an important consideration in hiring decisions, and feedback should be given to make the workplace more creative.
Relationships among students’ reading habits, study skills, and academic achievement in English at the secondary level
Authors: Abid Nisar , Aslam Sarfraz , Alghamdi Abdulelah A. , Kumar Tribhuwan 
Published: January 2023

IntroductionReading is an attempt to comprehend the writer’s message for personal growth and success in the relevant fields. Thus, psychologists consider it a multifaceted cognitive process of constructing meanings from texts. The present study was conducted to determine the relationships among students’ reading habits, study skills, and academic achievement in English at the secondary level in Punjab, Pakistan.MethodsThe (n = 1614) students enrolled in the science section for the academic year 2019–2020 participated in this descriptive correlational survey, selected from 40 high schools in Lahore, Punjab, Pakistan, through a non-proportionate stratified random sampling technique. The Reading Habits Questionnaire (RHQ) and the Study Skills Scale (SSS) were used to collect data about students’ reading habits and study skills. At the same time, academic achievement was the students’ grades obtained in the ninth class in the subject of English that were determined by the Board of Intermediate and Secondary Education (BISE) Lahore in 2019. Students’ responses were analyzed through descriptive and inferential statistics.ResultsThe results indicated that students have competent reading habits and study skills. The correlational findings showed a strong positive relationship among reading habits, study skills, and academic achievement in English, while moderate positive relationships between reading habits and academic achievement in English. However, regression analysis results were significant, while reading habits and study skills moderately predicted academic achievement.DiscussionIt is implicated that teachers should plan such assignments and tasks based on reflective thinking by considering the role of study skills in academic achievement. Moreover, teachers and school administrators could mutually create timetables for library lessons to build reading habits and study skills among learners.
Neighborhood conditions in a Swedish context-Two studies of reliability and validity of virtual systematic social observation using Google Street View
Authors: Clausén Gull Ingela , Kapetanovic Sabina , Norman Åsa , Ferrer-Wreder Laura , Olsson Tina M. , Eninger Lilianne 
Published: January 2023

IntroductionThe goal of these studies was to investigate the reliability and validity of virtual systematic social observation (virtual SSO) using Google Street View in a Swedish neighborhood context.MethodsThis was accomplished in two studies. Study 1 focused on interrater reliability and construct validity, comparing ratings conducted in-person to those done using Google Street View, across 24 study sites within four postal code areas. Study 2 focused on criterion validity of virtual SSO in terms of neighborhoods with low versus high income levels, including 133 study sites within 22 postal code areas in a large Swedish city. In both studies, assessment of the neighborhood context was conducted at each study site, using a protocol adapted to a Swedish context.ResultsScales for Physical Decay, Neighborhood Dangerousness, and Physical Disorder were found to be reliable, with adequate interrater reliability, high consistency across methods, and high internal consistency. In Study 2, significantly higher levels of observed Physical Decay, Neighborhood Dangerousness, and signs of garbage or litter were observed in postal codes areas (site data was aggregated to postal code level) with lower as compared to higher income levels.DiscussionWe concluded that the scales within the virtual SSO with Google Street View protocol that were developed in this series of studies represents a reliable and valid measure of several key neighborhood contextual features. Implications for understanding the complex person-context interactions central to many theories of positive development among youth were discussed in relation to the study findings.
Investigating the modulation of active preparation and passive dissipation on inhibitory control processes in the language switching paradigm
Authors: Shen Qinfang 
Published: January 2023

IntroductionPrevious language-switching studies have received scholastic attention and the observed switching cost patterns have provided empirical evidence for bilingual language control. However, results are inconsistent as the size of and (a)symmetry in switching costs differ across studies. In addition, there are various methodological differences that go beyond stimulus differences, such as the language proficiency of the participants (the participant-level factor) and the preparation time (a task-related level factor), which might be responsible for these inconsistent results.MethodsWith a focus on task-related factors, the present study was designed to examine whether and how preparation time modulates the size and (a)symmetry in switching costs by using the language-switching paradigm with cue-to-stimulus and response-to-cue intervals manipulated.ResultsReplicating previous literature on language switching and task switching, a clear preparation effect was observed in all trials (stay and switch trials) for both L1 and L2. The switching costs were modulated by the cue-to-stimulus intervals, and specifically, switching costs decreased when the preparation time increased. Another intriguing finding was that even when participants were offered enough time to fully prepare for selecting the target language at the cue window, the switching costs were not completely eliminated. In terms of the passive preparation at the response-to-cue interval, switching costs could be modulated by the response-to-cue interval – the time for passive dissipation of inhibitory control applied in previous trials. The size of switching costs was clearly modulated by manipulating response-to-cue intervals and switching costs decreased as the waiting time after a naming response increased.DiscussionThis study provides empirical evidence for the modulation of preparation effects on switching costs and inhibitory control mechanisms in bilingual language production.