bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photosynthetic demands on translational machinery drive retention of redundant tRNA metabolism in plant organelles
Authors: DeTar Rachael Ann , Chustecki Joanna , Martinez-Hottovy Anna , Ceriotti Luis Federico , Broz Amanda K. , Sanchez-Puerta M. Virginia , Elowsky Christian , Christensen Alan C. , Sloan Daniel B. 
Published: August 2023

AbstractEukaryotic nuclear genomes often encode distinct sets of translation machinery for function in the cytosol vs. organelles (mitochondria and plastids). This raises questions about why multiple translation systems are maintained even though they are capable of comparable functions and whether they evolve differently depending on the compartment where they operate. These questions are particularly interesting in plants because translation machinery, including aminoacyl-tRNA synthetases (aaRS), is often dual-targeted to the plastids and mitochondria. These organelles have different functions, with much higher rates of translation in plastids to supply the abundant, rapid-turnover proteins required for photosynthesis. Previous studies have indicated that plant organellar aaRS evolve more slowly compared to mitochondrial aaRS in eukaryotes that lack plastids. Thus, we investigated the evolution of nuclear-encoded organellar and cytosolic aaRS and tRNA maturation enzymes across a broad sampling of angiosperms, including non-photosynthetic (heterotrophic) plant species with reduced plastid gene expression, to test the hypothesis that translational demands associated with photosynthesis constrain the evolution of enzymes involved in organellar tRNA metabolism. Remarkably, heterotrophic plants exhibited wholesale loss of many organelle-targeted aaRS and other enzymes, even though translation still occurs in their mitochondria and plastids. These losses were often accompanied by apparent retargeting of cytosolic enzymes and tRNAs to the organelles, sometimes preserving aaRS-tRNA charging relationships but other times creating surprising mismatches between cytosolic aaRS and mitochondrial tRNA substrates. Our findings indicate that the presence of a photosynthetic plastid drives the retention of specialized systems for organellar tRNA metabolism.SignificanceThe process by which endosymbionts are integrated into a host and become organelles results in a combination of gene loss, transfer to the nucleus, and retention in the organellar genome. It is not clear why some endosymbiont-derived genes may be retained when a functional host counterpart exists whose gene product could simply be retargeted to the organelles. This study revealed that the photosynthetic activity in plant plastids may be responsible for retention of functionally redundant tRNA processing machinery, while mitochondria are more flexible regarding substitution with cytosolic-type enzymes. Therefore, functional constraint in the plastid is likely more important than in the mitochondria for shaping the evolution and retention of functionally redundant proteins that are dual targeted to both organelles.
Germ granule association drives small RNA specificity for a nuclear Argonaute protein
Authors: Chen Shihui , Phillips Carolyn M. 
Published: August 2023

AbstractRNA interference (RNAi) is a conserved gene silencing process that exists in diverse organisms to protect genome integrity and regulate gene expression. InC. elegans, the majority of RNAi pathway proteins localize to perinuclear, phase-separated germ granules, which are comprised of sub-domains referred to as P granules,Mutatorfoci, Z granules, and SIMR foci. However, the protein components and function of the newly discovered SIMR foci are unknown. Here we demonstrate that HRDE-2 localizes to SIMR foci and interacts with the germline nuclear RNAi Argonaute HRDE-1. Furthermore, HRDE-1 also localizes to SIMR foci, dependent on HRDE-2, but only in its small RNA unbound state. This germ granule localization is critical to promote the small RNA binding specificity of HRDE-1 and, in the absence of HRDE-2, HRDE-1 exclusively loads CSR-class 22G-RNAs rather than WAGO-class 22G-RNAs, resulting in inappropriate H3K9me3 deposition on CSR-target genes. Thus, our study demonstrates that the recruitment of unloaded HRDE-1 to germ granules, mediated by HRDE-2, is critical to ensure that the correct small RNAs are used to guide nuclear RNA silencing in theC. elegansgermline.
Robo1 loss has pleiotropic effects on postnatal development and survival
Authors: Kirk Nicole A. , Hwang Ye Eun , Lee Seul Gi , Kim Kee-Beom , Park Kwon-Sik 
Published: August 2023

AbstractRoundabout guidance receptor 1 (Robo1) is known to function during midbrain development in mice, but its postnatal role remains poorly defined in part due to the perinatal lethality of the mice lacking the gene. Here, we describe the postnatal phenotypes ofRobo1-/-mice in the B6/129s genetic background.Robo1-/-mice had both a slower growth rate and shorter lifespan compared toRobo1+/+littermates. Skin histological analysis revealed thatRobo1-/-mice displayed increased wrinkles, enlarged sebaceous glands, and a reduced subcutaneous fat layer.Robo1-/-mice displayed accelerated hair graying in line with an observed overall decrease in melanin production. Although both growth hormone (GH) and insulin-like growth hormone 1 (IGF-1) were suppressed inRobo1-deficient mice at 3 weeks of age, they were expressed at similar levels toRobo1intact mice at 7 weeks of age. These findings suggest thatRobo1-mediated hormone regulation is required for normal growth during the early stages of puberty and may provide a crucial timepoint at which to examine the molecular mechanisms of pituitary disorders.
Long-term impact of early life stress on serotonin connectivity
Authors: Ramkumar Raksha , Edge-Partington Moriah , Adigun Kabirat , Ren Yi , Terstege Dylan J , Khan Nazmus S , Rouhi Nahid , Jamani Naila F , Tsutsui Mio , Epp Jonathan R , Sargin Derya 
Published: August 2023

AbstractChronic childhood stress is a prominent risk factor for developing mood disorders, yet mechanisms underlying this association remain unclear. Serotonin (5-HT) plays a crucial role in neurodevelopment and vulnerability to mood disorders. Maintenance of optimal 5-HT levels during early postnatal development is critical for the maturation of brain circuits. Developmental stress can alter the serotonin system, leading to chronic behavioral deficits. Yet, our understanding of the long-term impact of early life stress (ELS) on serotonin connectivity remains incomplete. Using a mouse model of chronic developmental stress, we sought to determine how ELS impacts brain-wide serotonin activity and behavior in adulthood. We established that adult female and male mice exposed to ELS during the first postnatal week show heightened anxiety-like behavior. Usingin vivofiber photometry and c-fos dependent activity mapping, we found that ELS enhances susceptibility to acute stress by disrupting the brain-wide functional connectivity of the raphe nucleus and the activity of dorsal raphe serotonin neuron population, in conjunction with a profound increase in the orbitofrontal cortex (OFC) activity. We further identified that 5-HT release in the medial OFC during environmental challenge is disrupted in mice exposed to ELS. Optogenetic stimulation of 5-HT terminals in the mOFC elicited an anxiolytic effect in ELS mice in a sex-dependent manner. Our findings hold significant insight into the mechanisms underlying long-term brain connectivity deficits induced by ELS, with potential implications for developing targeted stimulation-based treatments for affective disorders that arise from early life adversities.
Examining generational differences in the workplace: narcissism, work centrality, and the impact on employee engagement and discretionary effort
Authors: Burawat Piyachat 
Published: August 2023

Purpose
The purpose of this study is to investigate the structural relationship model among narcissism, work centrality, employee engagement and discretionary effort.

Design/methodology/approach
Respecting the quantitative approach, the data were gathered by means of the survey completed by 1,190 respondents of Generation X, Y and Z working in the Thai service and manufacturing industries.

Findings
The findings of the study indicated positive associations between narcissism and work centrality, narcissism and employee engagement, work centrality and employee engagement and employee engagement and discretionary effort. It was observed that work centrality played a partially mediating role in the relationships between narcissism and employee engagement, while employee engagement fully mediated the associations between work centrality and discretionary effort, as well as between narcissism and discretionary effort. The structural model demonstrated variations across different generational cohorts, suggesting that the relationships differed among generations. However, the model did not exhibit any variations across different types of industries. Furthermore, the findings from the Thai samples indicated that generational differences had a moderating effect on the path relationships between narcissism and work centrality (with Generation Y having a stronger effect compared to Generation Z and Generation X), narcissism and employee engagement (with Generation Y having a stronger effect compared to Generation Z and Generation X) and narcissism and discretionary effort (with Generation X having a stronger effect compared to Generation Y and Generation Z), all with a significance level of 95%. Additionally, the relationship between employee engagement and discretionary effort showed a significant difference across generations, with Generation Z exhibiting a stronger effect compared to Generation Y and Generation X, at a significance level of 99%.

Originality/value
This study provides empirical evidence supporting the positive relationships between narcissism and work centrality, as well as narcissism and employee engagement, contrary to previous research findings. The results reveal that employee engagement fully mediates the associations between work centrality and discretionary effort, as well as between narcissism and discretionary effort, offering valuable contributions to the existing literature. Furthermore, this study expands on previous research by examining the moderating effect of industry type, specifically comparing manufacturing and service industries. The findings suggest that the model exhibits variations across different generational cohorts, highlighting the importance of considering generational differences in understanding these relationships. However, the model does not exhibit variations across different types of industries.
Entangling knowledge search and innovation targets for inventing emerging technologies: the case for blockchain technology
Authors: Huang Jingxuan , Dong Qinyi , Li Jiaxing , Kang Lele 
Published: August 2023

PurposeWhile the growth of emerging technologies like Blockchain has created significant market opportunities and economic incentives for firms, it is valuable for both researchers and practitioners to understand their creation mechanisms. This paper aims to discuss the aforementioned objective.Design/methodology/approachBased on the knowledge search perspective, this study examines the impact of search boundary on innovation novelty and quality. Additionally, innovation targets, namely R&D innovation and application innovation, are proposed as the moderator of the knowledge search effect. Using a combination of machine learning algorithms such as natural language processing and classification models, the authors propose new methods to measure the identified concepts.FindingsThe empirical results of 3,614 Blockchain patents indicate that search boundary enhances both innovation novelty and innovation quality. For R&D innovation, the positive impact of search boundary on innovation quality is enhanced, whereas for application innovation, the positive effect of search boundary on innovation novelty is improved.Originality/valueThis study mainly contributes to the growing literature on emerging technologies by describing their creation mechanisms. Specifically, the exploration of R&D and application taxonomy enriches researchers' understanding of knowledge search in the context of Blockchain invention.
The impact of corporate social irresponsibility media coverage on firm performance
Authors: Mendiratta Anita , Singh Shveta , Yadav Surendra S. , Mahajan Arvind 
Published: August 2023

PurposeThis paper aims to assess the impact of corporate social irresponsibility (CSiR) media coverage on firm performance in India. It also analyses the effects of the environment, social, governance, and cross-cutting issues on firm performance.Design/methodology/approachThe paper utilizes a sample of Indian firms from the Reprisk® database, amounting to 1,103 CSiR media coverage counts for 693 firm-year annual observations from 2008 to 2015. Further, Reprisk® segregates comprehensive CSiR coverage counts into the environment, social, governance and cross-cutting issues, for which the study runs the fixed effects panel regression. The study takes year-fixed effects, industry-fixed effects and clustered standard errors at the industry level.FindingsThe results of this study indicate that CSiR coverage negatively influences the firm performance of Indian firms. All issues, including social, governance and cross-cutting, except environmental issues, negatively impact firm value in India.Practical implicationsThe involvement of firms in CSiR costs the firms financially and drives down firm performance. Social issues, including community and employee-related matters, governance issues and cross-cutting issues, also reduce the firm performance.Social implicationsThe insignificant environmental impact on firm performance does not indicate that environmental issues have no detrimental consequences. Instead, it might need more stakeholders' awareness to understand the harmful implications of environmental issues on society.Originality/valueLimited studies have explored CSiR in India so far. The study is novel as it analyses the Reprisk® database and its segregation of media counts into the environment, social, governance and cross-cutting issues in the Indian context.
One for all, all for one: an analysis towards the speed of internationalization in clustered and non-clustered family firms
Authors: Mendes Telma , Braga Vitor , Silva Carina 
Published: August 2023

PurposeThis article aims to explore how cluster affiliation moderates the relationship between family involvement and speed of internationalization in family firms. The speed of internationalization is examined in terms of earliness and post-internationalization speed.Design/methodology/approachThe research is based on a sample of 639 Portuguese family businesses (FBs) created and internationalized between 2010 and 2018 that was retrieved from the Iberian Balance Analysis System – SABI database. The partial least squares structural equation modeling (PLS-SEM) was used to assess the measurement and construct the model.FindingsThe results suggest that higher levels of family involvement in ownership and management make family firms enter on international markets in later stages of their development but, after the first international market entry, the firms are able to exhibit a higher post-internationalization speed. When considering the effect of cluster affiliation, the authors found that clustered FBs are more likely to engage in early internationalization and to accelerate the post-internationalization process than non-clustered FBs.Originality/valueThe study's findings are explained by the existence of socially proximate relationships with other cluster members, based on similarity, trust, knowledge exchange and sense of belonging, which push family firms to internationalize and increase their level of international commitment over time. The empirical evidence, therefore, highlights the primary role of industrial clusters in moderating the relationship between family involvement, earliness of internationalization and post-internationalization speed.
Ofsted suicides: who is responsible for suicide prevention?
Authors: Waters Sarah , Palmer Hilda 
Published: August 2023

PurposeThe purpose of this paper is to discuss the response of the relevant authorities to evidence that female primary schoolteachers have an elevated suicide risk in the UK. The paper situates the recent tragic death of a primary school head teacher, following an Ofsted inspection at her school, within the wider context of teacher suicide deaths and asks what, if any, action the authorities have taken to prevent avoidable suicide deaths from occurring.Design/methodology/approachThe paper examines a recent case of suicide by a primary head teacher within the wider context of statistical data on suicides by primary schoolteachers and in relation to previous cases of suicide linked to a school inspection.FindingsThe paper suggests that the relevant authorities have failed to act in relation to evidence of high suicide risk amongst female primary schoolteachers and to previous suicide deaths linked to the impact of a school inspection. Without learning from suicide deaths and acting on available evidence, there is a risk that preventable suicide deaths will continue to occur.Originality/valueThe paper draws together case study evidence and statistical data to make the case for regulatory reform to ensure that work-related suicides are investigated, monitored and prevented.
Corporate digital responsibility (CDR) in the age of AI: implications for interactive marketing
Authors: Kunz Werner H. , Wirtz Jochen 
Published: August 2023

PurposeDespite all the recent achievements in the field of interactive marketing and artificial intelligence (AI), it is important to consider the ethical implications of these technologies. This paper explains the concept of corporate digital responsibility (CDR) and how it is affected by new advances in AI.Design/methodology/approachThe authors build on the work of Wirtz et al., (2023) and derive several managerial implications for the challenges that AI poses to CDR. CDR refers to a service company's ethical and fair use of data and technology within its digital service ecosystem. It involves establishing standards, protecting customer privacy, conducting external audits and striving for an equitable power dynamic between service firms and their partners.FindingsDespite the risks involved, many companies are not prioritizing good CDR practices. Financial benefits from the collection and use of consumer data, improved customer experience through AI-driven customization and personalization, cost reduction through service automation and the trade-offs between organizational goals and CDR practices can prevent companies from prioritizing good CDR practices.Originality/valueThis is one of the first articles in the service domain to take the concept of CDR and apply it to recent developments in generative AI.Research limitations/implicationsThe emergence of powerful AI tools presents opportunities and challenges. Research opportunities include responsible business restructuring, responsible service automation to ensure fairness and human oversight, addressing dehumanization of service delivery, responsible customer profiling to address privacy and discrimination concerns and preventing AI misuse.
Two decades of brand hate research: a review and research agenda
Authors: Kumar Ramesh , Jebarajakirthy Charles , Maseeh Haroon Iqbal , Dhanda Komal , Saha Raiswa , Dahiya Richa 
Published: August 2023

PurposeThis review aims to synthesize the brand hate literature and suggest directions for future research on brand hate.Design/methodology/approachThis study adopted an integrative literature review method to synthesize and assess the brand hate literature.FindingsThe synthesis showed that social identity theory, disidentification theory and duplex theory are prominently used in brand hate studies, and a larger portion of brand hate research was conducted in Western countries. Further, brand-related, self-congruity, personal factors, information influence and brand community influence are the major types of antecedents of brand hate which can produce soft or hard consequences. Lexicometric analysis showed causes of brand hate, consumers' negative emotional and behavioral outcomes and community anti-brand behavior as key themes of brand hate research.Research limitations/implicationsThe synthesis has followed predefined criteria for the inclusion research papers. Thus, the review is limited to articles that fulfilled the criteria for inclusion.Practical implicationsThe finding will help marketers, specially brand managers, craft strategies to handle brand hate.Originality/valueThe brand hate literature is still developing and remains incoherent, suggesting that a synthesized review is needed. This study has systematically reviewed and synthesized the brand hate literature to study its development over time and proposes a framework which provides a comprehensive understanding of brand hate.
Athlete apparel: the impact of team brand awareness and apparel brand awareness on athletes' brands, logos, and merchandise
Authors: Williams Antonio , Pedersen Zack Paul 
Published: August 2023

PurposeBranded merchandise and licensed apparel comprise a substantial portion of revenue for many organizations and public figures that choose to employ such an endeavor. Endorsement deals with apparel manufacturers have historically been utilized for athletes looking to supplement their salaries and establish greater brand awareness. However, as some athletes establish ownership of their logo and become less reliant on companies such as Nike and Adidas for merchandise distribution, assessing the influence of various entity's logos on the athlete brand has become worthy of analysis. Therefore, this study aimed to investigate the influence that cobranded merchandise has on consumers when the athlete logo is displayed next to another team or manufacturer logo.Design/methodology/approachUsing an online panel and survey, a final sample of 127 participants completed a questionnaire to examine their attitudes towards various athlete brand elements. ANCOVA's and MANCOVA's were utilized to assess significant findings, holding the variable of identification constant.FindingsThe results revealed that only the perceptions of merchandise quality significantly varied between an athlete brand and an apparel manufacturer (i.e. Nike) co-brand. The findings indicate that athletes should look to co-brand with high brand awareness manufacturers, and that there is no significant difference between consumers' perceptions of athlete brands when co-branding with team brands.Originality/valueThis is one of the first studies to evaluate the relationship between the athlete brand and external entities from a consumer perspective.