bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative Bioinformatics Analysis Reveals the Key Molecular Players in Metastatic Adrenocortical Carcinoma
Authors: Zhang Yiyi , Zhu Ying , Tang Nie 
Published: June 2023

AbstractAdrenocortical carcinoma (ACC) is an uncommon, possessive, and highly metastasizable malignancy of the adrenal cortex. Using bioinformatics analysis of microarray datasets, this work is intended to uncover important molecular entities and pathways complicated in ACC metastasis. Three datasets (GSE90713, GSE143383, and GSE19750) were obtained from the Gene Expression Omnibus (GEO) database, comprising a total of 226 ACC samples and healthy controls. A collection of differentially expressed genes (DEGs) and differentially expressed miRNAs (DEmiRs) linked with ACC and ACC metastases was found using differential gene expression analysis. Functional enrichment analysis revealed enriched pathways such as "Staphylococcus aureus infection," "Phagosome," "Cell adhesion molecules," and "Pathways in cancer," indicating potential mechanisms underlying ACC metastasis. Hub genes with potential involvement in ACC metastasis were discovered by protein-protein interaction (PPI) network analysis, includingGAPDH,MYC,VEGFA,CDC20,CCL2,MMP9,ITGAM,DLGAP5,KIF2C, andFCGR3A.CCL2,CDC20,DLGAP5,KIF2C,MMP9, andMYCwere shown to be substantially linked with the prognosis and overall survival of ACC patients by survival analysis. A network was identified between targeted hub genes and DEmiRs. These findings provide insight into the molecular mechanisms of ACC metastasis as well as potential therapeutic targets for further targeted therapies. The identified hub genes and pathways may also have implications for the understanding and treatment of other types of cancer.
Causal Effects of Gut Microbiome on Autoimmune Liver Disease: A Two-Sample Mendelian Randomization Study
Authors: Fu Yugang , Li Jiacheng , Zhu Yingying , Chen Chong , Liu Jing , Gu Simin , Zheng Yiyuan , Li Yong 
Published: June 2023

Abstract
Background
Epidemiological studies have indicated a potential link between the gut microbiome and autoimmune liver disease (AILD) such as autoimmune hepatitis (AIH), primary biliary cholangitis (PBC), and primary sclerosing cholangitis (PSC). The relationship between the gut microbiome and autoimmune liver disease is still uncertain due to confounding variables. In our study, we aim to shed light on this relationship by employing a two-sample Mendelian randomization approach.
Methods
We conducted a two-sample Mendelian randomization (MR) study using the R package "TwoSampleMR". The exposure data consisted of genetic variants associated with 194 bacterial traits obtained from the MiBioGen consortium. Summary statistics for AILD were obtained from the GWAS Catalog website. Furthermore, a series of sensitivity analyses were performed to validate the initial MR results.
Results
There were two, four and three bacteria traits associated with an increased risk of AIH. PBC, and PSC respectively. In contrast, there were five, two and five bacteria traits associated with a decreased risk for AIH, PBC and PSC. Notably, the genus_Clostridium_innocuum_group showed a negative association with AIH (OR = 0.67, 95% CI: 0.49–0.93), and the genus_Actinomyces was found to be genetically associated with a decreased risk of PSC (OR = 0.62, 95% CI: 0.42–0.90).
Conclusions
Our study identified the causal impact of specific bacterial features on the risk of AILD subtypes. Particularly, the genus_Clostridium_innocuum_group and the genus_Actinomyces demonstrated significant protective effects against AIH and PSC respectively. These findings provide further support for the potential use of targeted probiotics in the management of AILD.
Design aspects and Performance analysis of Multicarrier Waveform contenders of 5G and beyond Wireless Networks
Authors: Jolania Smita , Sindal Ravi , Saxena Ankit 
Published: June 2023

Abstract
The next generation wireless network has to cope with the diversified requirements like high data rate, improved quality of service, heterogeneous traffic, low latency and high reliability in the limited available spectrum. With the objective of enhancing the spectrum efficiency and providing the diverse services, the design of air interface needs to be considered. The waveform to be implemented at the physical layer in 5G must be able to perform in high dense networks with high throughput. The key challenge in selecting the modulation waveform is that it must be flexible, spectral efficient and compatible with the service required. In this paper various design parameters of most suitable multi carrier waveform: Filtered Orthogonal Frequency Division Multiplexing (F-OFDM), Universal Frequency Division Multiplexing (UFMC), Filter Bank Multi Carrier (FBMC), and Generalized Frequency Division Multiplexing (GFDM) have been investigated and analyzed. Parameterized waveforms are simulated and performance analysis on the basis of Bit error rate (BER) and Power Spectral Density (PSD) is presented. In addition to this the compatibility and complexity of the modulation technique in different 5G use cases is elaborated.
Principle, equipment and applications of line-scanning infrared thermographic NDT
Authors: Vavilov V. P. , Chulkov A. O. , Nesteruk D. A. , Kladov D. Yu. 
Published: June 2023

Abstract
The Line Scan Thermography (LST) technique for thermal nondestructive testing (TNDT) uses continuous heating from an incandescent (quartz) linear tube heater. This provides a continuous linear inspection with a lower noise level than the classical single area Xenon flash TNDT technique. The basic theory of the LST technique is described, along with a description of a special, self-propelled LST device. It is shown that TNDT processes using a moving heat source can be evaluated by solving simpler, static TNDT problems using flash heating. An example of the experimental detection of hidden corrosion in steel is presented. The LST method is recommended for the continuous inspection of large objects, especially flat, cylindrical and/or conical shapes.
Understanding health systems challenges in providing Advanced HIV Disease (AHD) care in a hub and spoke model: A qualitative analysis to improve AHD care program in Malawi.
Authors: Maphosa Thulani , Denoeud-Ndam Lise , Kapanda Lester , Khatib Sarah , Chilikutali Llyod , Matiya Eddington , Munthali Boswell , Dambe Rosalia , Chiwandira Brown , Wilson Bilaal , Nyirenda Laywell , Chikwapulo Bongani , Tiam Appolinaire , Katirayi Leila 
Published: June 2023

Abstract
Background
Despite tremendous progress in improving antiretroviral therapy (ART) and access to ART, many patients have advanced HIV disease (AHD). AHD patients, whether initiating ART or representing care after disengagement, have an increased risk of morbidity and mortality. EGPAF launched an enhanced care package using a hub and spoke model to optimize AHD care in Malawi. This model improved supplies availability and appropriate linkage to care. We utilize a hub-and-spoke model to share the health facility challenges and recommendations on the AHD package for screening and diagnosis, prophylaxis, treatment, and adherence support.
Methods
This qualitative study assessed facility-level experiences among healthcare workers (HCWs) and lay cadres (LCs) providing AHD services to patients through the intervention package. The study population included HCWs and LCs supporting HIV care at four intervention sites. Eligible study participants were recruited by trained Research Assistants with support from the health facility's nurse to identify those most involved in supporting AHD patients. A total of 32 in-depth interviews were conducted. Thematic content analysis identified recurrent themes and patterns across participants’ responses.
Results
While HCWs and LCs stated that most medications are often available in both hub and spoke sites, they reported that sometimes there are limited supplies and equipment to run samples and tests necessary to provide AHD care. More than half of the HCWs stated that AHD training sufficiently prepared them to handle AHD patients at both hubs and spoke levels. The HCWs and LCs reported weaknesses in the patient referral system within the hub-and-spoke model in providing linkage of care to facilities, specifically noting improper referral documentation, incorrect labeling of samples, and inconsistent availability of transportation. While HCWs felt that AHD registers were time-consuming, they remained motivated as they thought they provided better patient services.
Conclusions
These findings suggest the importance of offering more comprehensive AHD services. The enhanced AHD program addressed weaknesses in service delivery through decentralization and providing services through a hub and spoke model, improved supplies availability, and strengthened linkage to care. Additionally, addressing the recommendations from service providers and patients is essential in improving health and survival among AHD patients.
Mechanisms of the plastoquinone pool oxidation in the dark after illumination
Authors: Vilyanen Daria , Naydov Ilya , Ivanov Boris , Kozuleva Marina , Borisova-Mubarakshina Maria 
Published: June 2023

Abstract
Oxidation of the PQ-pool in leaves, intact chloroplasts and isolated thylakoids after its reduction by flash of light intensity 3000 µmol photons m− 2 s− 1 for 1.5 s was studied as a time-dependence in the dark of area above curve of fluorescence, Afl. The fluorescence was excited with the same flash applied after dark intervals from 0.1 s to 10 min. The samples were either dark-adapted or pre-illuminated with a continuous light. The results showed a two-phase kinetic of Afl across all samples, consistent with prior findings in thylakoids without added electron acceptors and in leaves. A comprehensive analysis of the kinetics in isolated thylakoids conducted under both aerobic and anaerobic conditions, with or without Fd and NADP+, and in the presence of catalase, enabled the proposition of mechanisms responsible for the observed two phases. Our findings suggest that the “fast” phase is likely a combination of residual electron flow from the PQ-pool to downstream acceptors and the oxidation of PQH2 by the superoxide radical, formed during illumination and preserved in thylakoid membrane. The "slow" phase involves the oxidation of the PQ-pool through slow autocatalytic reactions with molecular oxygen, and this oxidation is enhanced in the presence of hydrogen peroxide. The role of the above processes in intact chloroplasts and leaves is discussed.
Acute effects of a warm-up intervention on pain, productivity, physical capacities and perceptions among vineyard workers: a cluster randomized trial
Authors: Larinier Nicolas , Vuillerme Nicolas , Jadaud Alexandre , Malherbe Solène , Giraud Eymeric , Balaguier Romain 
Published: June 2023

Abstract
Purpose Agriculture is one of the sectors that are the most concerned by musculoskeletal disorders (MSDs). Workplace physical activity programs are one of the most promising solutions to prevent adverse consequences of MSDs such as pain or impairment in physical capacities. Despite the growing interest to the implementation of warming-up sessions before the working day, the available evidence of their effectiveness is still scarce. The aims of this study were twofold: (1) to investigate the acute effect of a warm-up session on pain, work performance, and physical and psychosocial capacities among vineyard workers; (2) to determine the warm-up modality the most beneficial to vineyard workers.Methods A cluster randomized study was implemented among French vineyard workers. A 15min single session of warm-up was implemented among four groups corresponding to four different conditions: (1) hybrid warm-up intervention (HWU); (2) dynamic warm-up intervention (DWU); (3) stretching warm-up intervention (SWU); (4) no warm-up intervention (CONTROL). A total of 92 vineyard workers were included in the analysis.Results DWU showed increased performance, increased heart rate, better readiness to work and lower workload than the CONTROL. HWU showed a better readiness to work. SWU showed better work quality. However, the three different warm-up protocols did significantly not differ from the CONTROL group in terms of perceived pain intensity, and physical capacities.Conclusion The present findings confirm some beneficial acute effects on performance, heart rate and perceptions of a single warm-up session performed before pruning. The DWU seems to be the warm-up modality the most beneficial to vineyard workers.Trial registration: NCT05425693. Registered 06/16/2022 in ClinicalTrials.gov
Extracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice
Authors: Schabussova Irma , Schmid Anna Marlene , Razim Agnieszka , Wysmolek Magdalena , Kerekes Daniela , Haunstetter Melissa , Kohl Paul , Brazhnikov Georgii , Geisler Nora , Thaler Michael , Krčmářová Eliška , Šindelář Martin , Hrdý Jiří , Schmidt Katy , Nejsum Peter , Whitehead Bradley , Palmfeldt Johan , Schild Stefan , –Kanada Alksandra Inić , Wiedermann Ursula 
Published: June 2023

Abstract
Background
E. coli O83 (Colinfant Newborn) is a Gram-negative (G-) probiotic bacterium used in the clinic. When administered orally, it reduces allergic sensitisation but not allergic asthma. Intranasal administration offers a non-invasive and convenient delivery method. This route bypasses the gastrointestinal tract and provides direct access to the airways, which are the target of asthma prevention. G- bacteria such as E. coli O83 release outer membrane vesicles (OMVs) to communicate with the environment. Here we investigate whether intranasally administered E. coli O83 OMVs (EcO83-OMVs) can reduce allergic airway inflammation in mice.
Methods
EcO83-OMVs were isolated by ultracentrifugation and characterised their number, morphology (shape and size), composition (proteins and lipopolysaccharide; LPS), recognition by innate receptors (using transfected HEK293 cells) and immunomodulatory potential (in naïve splenocytes and bone marrow-derived dendritic cells; BMDCs). Their allergy-preventive effect was investigated in a mouse model of ovalbumin-induced allergic airway inflammation.
Results
EcO83-OMVs are spherical nanoparticles with a size of about 110 nm. They contain LPS and protein cargo. We identified a total of 1120 proteins, 136 of which were enriched in OMVs compared to parent bacteria. Proteins from the flagellum dominated. OMVs activated the pattern recognition receptors TLR2/4/5 as well as NOD1 and NOD2. EcO83-OMVs induced the production of pro- and anti-inflammatory cytokines in splenocytes and BMDCs. Intranasal administration of EcO83-OMVs inhibited airway hyperresponsiveness, and decreased airway eosinophilia, Th2 cytokine production and mucus secretion.
Conclusions
We demonstrate for the first time that intranasally administered OMVs from probiotic G- bacteria have an anti-allergic effect. Our study highlights the advantages of OMVs as a safe platform for the prophylactic treatment of allergy.
Risk factors of secondary cancer in laryngeal, oropharyngeal, or hypopharyngeal cancer after definitive therapy
Authors: Nishimura Goshi , Takahashi Hideaki , Sano Daisuke , Arai Yasuhiro , Hatano Takashi , Kitani Yosuke , Oridate Nobuhiko 
Published: June 2023

Abstract
Background
Our previous research showed that a high rate of secondary carcinogenesis is observed during follow-up after transoral surgery in patients with early-stage laryngeal, oropharyngeal, and hypopharyngeal cancers. We speculate that the contributing factors are alcohol drinking, smoking, and aging; however, we could not provide clear evidence. In this study, we aimed to identify the risk factors for secondary carcinogenesis in patients with these cancers, particularly factors associated with drinking and/or smoking.
Methods
The medical records of all-stage laryngeal, oropharyngeal, and hypopharyngeal cancer patients who had undergone definitive treatment were retrospectively analyzed. Assessments included visual and endoscopic observations of the primary site, enhanced cervical CT or US of the primary site and regional lymph nodes, PET-CT, and enhanced whole-body CT. Clinical characteristics were compared in patients with and without secondary carcinogenesis and in patients with hypopharyngeal cancer and patients with other cancers.
Results
Hypopharyngeal cancer was an independent risk factor for secondary cancer. The 5-year incidence rate of secondary cancer was 25.5%, 28.6%, and 41.2% in laryngeal, oropharyngeal, and hypopharyngeal cancers, respectively. Radiotherapy was defined as an independent risk factor in hypopharyngeal cancer patients with secondary cancers. No direct correlation was found between secondary carcinogenesis and alcohol consumption, smoking, or aging.
Conclusions
Patients with hypopharyngeal cancer require close follow-up as they are at high risk of developing secondary cancer, possibly because out-of-field radiation exposure may induce systemic secondary carcinogenesis in hypopharyngeal cancer patients with genetic abnormality induced by alcohol consumption.
The saltier Atlantic-fresher Pacific trend enhanced by oceanic processes
Authors: Lu Ying , Li Yuanlong , Lin Pengfei , Cheng Lijing , Liu Hailong , Duan Jing , Wang Fan 
Published: June 2023

AbstractBroad-scale salinity changes have been observed in oceans since the mid-20thcentury. One salient feature is the salinity increase of the Atlantic Ocean (AO) in contrast to the salinity decrease of the Pacific Ocean (PO). Our analysis of observational datasets suggests that this “saltier Atlantic-fresher Pacific” (SAFP) trend occurs primarily in the upper 800 m of the 45°S-45°N band, with notable impacts on regional sea level and ocean stratification. In addition to the surface freshwater fluxes stressed by existing studies, heat- and wind-driven oceanic processes have also vitally contributed to this pattern. Specifically, sea surface warming leads to poleward migration of outcrop zones of the thermocline, while changes in surface winds and overturning circulations cause upper-layer convergence in low- and mid-latitudes. These processes drive basin-scale salt redistribution that involves substantial salinity increases in the 45°S-45°N band of the AO but much weaker signatures in the PO. These effects are confirmed by sensitivity experiments of an ocean-sea ice model. Although climate models can broadly replicate the SAFP pattern, they tend to underestimate the AO salinity increase due to model biases in salinity climatology. This work highlights the essence of ocean processes in the response of ocean salinities to climate change and provides a benchmark for climate models.
Impact of the prognostic nutritional index on renal replacement therapy–free survival and mortality in patients on continuous renal replacement therapy
Authors: Lee Yu-Fu , Lin Pei-Ru , Wu Shin-Hwar , Hsu Hsin-Hui , Mao I-Chieh , Kor Chew-Teng 
Published: June 2023

Abstract

The survival of critically ill patients with acute kidney injury (AKI) undergoing continuous renal replacement therapy (CRRT) is highly dependent on their nutritional status. This retrospective cohort study investigated the correlation between the prognostic nutritional index (PNI) and clinical outcomes of critically ill patients with AKI receiving CRRT. We analyzed data from 2076 critically ill patients admitted to the intensive care unit at Changhua Christian Hospital, a tertiary hospital in central Taiwan, between January 1, 2010, and April 30, 2021. All these patients met the inclusion criteria of the study. Through Cox proportional hazard model and propensity score matching, the relationship between PNI and renal replacement therapy–free survival (RRTFS) and mortality was examined. The results revealed that patients in the high PNI group had significantly higher RRTFS rates and higher survival rates compared with those in the low PNI group. Although parenteral nutrition (PN) did not provide significant survival benefits, long-term PN may improve the nutritional status and reduce mortality in AKI patients on CRRT. Therefore, PNI, which measures albumin and lymphocytes, is a simple and effective method that can be used to assess nutritional status and predict outcomes in clinical settings.
The Molecular Docking Study of Interaction of Newly Synthesised Benzamide Appended by Pyrazolone Derivatives as Ligand Molecule With the Target Protien 6lu7 of Novel Corona Virus
Authors: V Smt. PAVITHRA , S SUDHA B 
Published: June 2023

Abstract
Plants and bioactive compounds have played an important role in the development of several clinically useful therapeutic agents since time immemorial. The global health emergency of novel COVID-19 is due to severe acute respiratory syndrome corona virus-2 (SARS-CoV-2). As if now there are no approved drugs for the treatment of corona viral disease (COVID-19), although some of the drugs have been tried. The virtual interaction of the COVID-19 main protease in complex with the inhibitor N3 (Research Collaborators for Structural Bioinformatics Protein Data Bank [PDB] ID: 6LU7), Hence this is chosen as target protein molecule. The newly synthesised compounds of benzamide appended pyrazolones derivatives are made as ligand molecules. The synthesis of these organic ligand molecules is done through four steps using different reagents and different environmental conditions. In this article we have discussed the interaction of our synthesized ligands with the target protein molecule using autodock tools. Firstly, the ligands were prepared using commercial ACD/chemsketch tool in PDB format. Desired protein target 6LU7 is downloaded from Protein Data Bank. Further protein optimization done by removing co-ordinates and hetero atoms, energy minimization of protein is done by swiss PDB viewer 4.1.0. To change the file format open babel 2.4.1 is used. Docking of protein and ligand is done using autodock 4.2 and the results are visualized by pymol and tabulated using the Lamarckian genetic algorithm. After docking comparative study of the binding energies, inhibitory constant and hydrogen bonding of all interactions were discussed and tabulated for the good results.
Probabilistic Computing with NbOx Mott Memristor-based Self-oscillatory pbit
Authors: Rhee Hakseung , Kim Gwangmin , Song Hanchan , Park Woojoon , Kim Do Hoon , In Jae Hyun , Kim Kyung Kim 
Published: June 2023

Abstract
Energy-based computing is a promising approach for solving NP-hard problems. Probabilistic computing using pbits, which can be fabricated through the semiconductor process and integrated with conventional processing units, can be an efficient candidate for fulfilling these demands. Here, we propose a novel pbit unit comprising a NbOx mott memristor-based oscillator, capable of generating probabilistic bits in a self-clocking manner. The noise-induced mott transition causes the probabilistic behavior, which can be effectively modeled using a multi-noise-induced stochastic process around the mott transition temperature. We demonstrate a memristive Boltzmann machine based on our proposed pbit and validate its feasibility by solving NP-hard problems. Furthermore, we propose a streamlined operation methodology that considers the autocorrelation of individual bits, enabling energy-efficient high-performance probabilistic computing.