bluuebunny/crossref_metadata_2025_split huggingface dataset:
Explore the prognostic characteristics of immunogenic cell death- related genes in colon carcinoma based on multi-constraint canonical correlation analysis
Authors: Xiong Xinyi , Cao Qian , Tang Jiangnan , He Lan , Shi Hongjian , Zhou Fangliang , He Yingchun 
Published: April 2023

Abstract
The prognosis for colon carcinoma is poor, it is a highly aggressive tumor, and its early symptoms are subtle. Hence, the need for more colon cancer prognostic indicators is urgent. Many long non-coding RNAs (lncRNAs) are known to actively contribute to the detection, prognosis, and treatment of tumors. On the other hand, immunogenic cell death (ICD) is vital for the prevention of tumors. This study seeks to research the mechanism of action of ICD-related lncRNAs in colon cancer and integrate ICD-related genes with lncRNAs. In order to investigate ICD-related lncRNAs, this research suggests a multi-constraint canonical correlation analysis. The prognostic model of the most significant lncRNAs is also constructed and verified in this paper using univariate and Lasso-Cox regression. In this study, colon cancer samples are split into high-risk and low-risk groups based on the model's risk score, and the impact of the model is assessed using the TCGA queue and the GEO queue, respectively. Also, we thoroughly examined the parallels and discrepancies in immune infiltration, functional enrichment, and medication sensitivity across the two risk groups. In conclusion, the ICD-related lncRNAs risk model developed in this research can serve as a guide for predicting the prognosis and therapeutic sensitivity of colon cancer patients.
Survival and predictors of mortality among HIV-infected adults after initiation of Anti-Retroviral Therapy in Eastern Ethiopia
Authors: Argaw Girum Shibeshi , Gelaye Proffessor Kasahun Alemu , Lakew Ayenew Molla , Aragaw Fnatu Mamo , Chekol Yazachew Moges , Tesfie Tigabu Kidie , Gelaw Negalign Byadge 
Published: April 2023

Abstract

Background: Human immune deficiency virus (HIV) remains the leading cause of morbidity and mortality globally. It can lead to AIDS, which results in gradual deterioration and failure of the immune system. As the immune system becomes compromised, the patient becomes highly susceptible to life-threatening infection which ends with early death. Even though antiretroviral therapy (ART) significantly decreases mortality as a whole, the rate of death is still the highest, especially in the first and second years of ART initiation.
Objectives: To assess the survival and predictors of mortality among HIV-infected adults after initiation of anti-retroviral therapy in Jigjiga Governmental Hospitals, Eastern Ethiopia.
Method: Institution-based Retrospective follow-up study was employed among ART patients from January 1, 2015, to December 31, 2021. Data were cleaned and entered in Epi-data version 3.1 and exported to STATA 14 for further analysis. Kaplan–Meier and Log-Rank tests were applied to compare survival differences among categories of different variables. In bi-variable analysis, p-values < 0.20 were included in a multivariable analysis. A multivariate Cox regression model was used to measure the risk of death & identify the significant predictors of death. Variables that p-value < 0.05 were considered statistically significant predictors of mortality.
Result in this study 466(53.34%) participants were male and 552(65.56 %) were urban residents about 91(10.81%) have died with an overall incidence rate of 3.92 (95% CI (2.4–5.4)) per 100-person year of observation. The overall survival probability of the study group was 83.97%. In the multivariable Cox regression analysis, baseline WHO stage III/IV (AHR=2.42(1.43-4.09)) have no caregiver (AHR=2.23; 95% CI (1.16-4.29)), being bedridden functional status (AHR=2.18; 95% CI (1.01-4.72)), and poor last known adherence level (AHR=4.23; 95%CI (2.39-7.47)) were found to be significant predictors of mortality.
Conclusion: the incidence of death was relatively high, especially in the second year of ART start. Baseline clinical WHO stage Ⅲ/Ⅳ, bedridden functional status at enrolment, and absence of caregiver, poor level of recent adherence was found to be independent predictors of mortality. Patients with these risk factors need special attention and are crucial to reducing the rate of mortality.
Esophageal cancer-derived exosomes imbalance Tfh/Tfr cell ratio in the tumor immune microenvironment via EXO-PDL1 to promote immunosuppression
Authors: Li Zijie , Zhang Yuehua , Hao He , Lv Tingting , Zhang Xiaokuan , Chen Lu , Qi Yuying , Wang Zhiyu 
Published: April 2023

Abstract
Background: Esophageal cancer (EC) is a deadly malignancy. Exosomal programmed death ligand 1 (EXO-PDL1) induces immune escape to promote tumor progression. Furthermore, the imbalance between follicular helper T cells (Tfh) and follicular regulatory T cells (Tfr) numbers is related to the progression of many malignant tumors. However, the role of the EC-derived EXO-PDL1 in Tfh/Tfr ratio is unknown.
Methods: Tfh and Tfr numbers in samples obtained from 45 patients with EC and 33 healthy donors (HD) were determined using flow cytometry. Exosomes were isolated using differential centrifugation from patients’ plasma and PDL1 expression on exosomes was tested using ELISA. Exosomes were cultured in vitro for Tfh and Tfr cells expansion assays. CD4+ T cells were isolated, stimulated, and cultured in vitro with exosomes to evaluate the levels, phenotypes, and functions of Tfh and Tfr cells.
Results: In patients with EC, the proportion of Tfh cells was lower than that in HD (P<0.001) whereas the proportion of Tfr cells was higher than that in HD (P<0.001). Patients with EC also showed a significantly lower ratio of Tfh/Tfr cells and a higher level of EXO-PDL1 than HD did (P<0.001). Additionally, a negative correlation was noted between EXO-PDL1 and Tfh/Tfr (R=-0.74, P<0.05). EC cell derived EXO-PDL1 inhibited the expansion of Tfh cells and enhanced the percentage of CTLA4+Tfh cells. Moreover, the levels of IL-21 and IFN-γ decreased, whereas IL-10 level was increased in response to EC cell derived EXO-PDL1. EXO-PDL1 promoted the expansion and suppressive functions of Tfr cells, the increased percentages of CTLA4+ Tfr cells and ICOS+ Tfr cells were accompanied with higher levels of IL-10, IFN-γ, and IL-21. Finally, EC derived exosomes promoted the imbalance of Tfh/Tfr ratio via the EXO-PDL1.
Conclusions: Patients with EC have imbalanced Tfh/Tfr ratio, which is attributed to EC-derived EXO-PDL1. Our results suggest a novel mechanism of EXO-PDL1-mediated immunosuppression in EC. Thus, inhibiting EXO-PDL1 to restore Tfh/Tfr cell balance may provide new therapeutic approaches in EC treatment.
Analysis of the optimal ratio of heat-insulating materials on the surface of heat-resistant rings in high ground temperature roadways
Authors: Deng Hongwei , ran chunzhen 
Published: April 2023

Abstract
The problem of heat damage in deep mine has become a hot issue in deep mining, and the research of reasonable and efficient heat barrier structure and better performance heat insulation materials can reduce the influence of high temperature environment to some extent. Firstly, based on the principle of heat transfer, we designed an overall coupled thermal barrier ring based on the analysis of the heat transfer mechanism of the surrounding rocks and carried out an in-depth analysis of the main factors of the structure, and developed a lightweight and durable thermal insulation material by mixing a high proportion of tailing with the solid waste conditions of the mine. Secondly, orthogonal tests were carried out to test the thermal parameters of different ratios of insulation materials, and the optimal horizontal ratio was obtained based on various analysis methods and consideration of the priority of each factor: 60 parts of tailings, 10% of glass beads, 2 parts of foaming agent, 15 parts of cement, and1% of cementitious materials with polyantelic acid water reducing agent. The study shows that the composite surrounding rock thermal barrier ring designed for the working conditions and the optimal ratio of surface insulation materials obtained from orthogonal test analysis can realize active cooling, create a comfortable environment for underground operation while saving costs, and also provide suggestions of measures for the management of thermal environment in the prominent thermal hazard mine.
Effect of Support on Palladium Catalyst for Aqueous Phase Hydrogenation of Maleic Acid to Succinic Acid
Authors: Ye Bin , Sun Siming , Wang Hui , Huang Huijiang , Rehman Moeez ur , Sun Xinyi , Xu Yan , Zhao Yujun 
Published: April 2023

Abstract
The synthesis of succinic acid (SA), one of the monomers for producing Poly (butylene succinate), which is a type of biodegradable plastic, has become a crucial and even bottleneck technology for the industry's development. Herein, the Pd-based catalysts were prepared with amorphous TiO2, CeO2 and ZrO2 as the supports and applied in maleic acid (MA) hydrogenation to SA in aqueous phase at a low temperature about 60 oC. It was found that the activity of Pd/CeO2 catalysts is significantly higher than that of Pd/TiO2 and Pd/ZrO2 catalysts. The strong interaction between Pd and CeO2 inhibited the agglomeration of Pd during the reduction and reaction process, leading to the highest Pd dispersion among the three catalysts. The exposure of more active sites for the adsorption of H2 endows it high activity in the process of MA hydrogenation to SA.
Detection of gut microflora and enzymes using metagenomics and metaproteomics in silkworm, Bombyx mori
Authors: Mohanraj Ponnusamy , Mahalingam C Aruchamy , Karthikeyan Chinnan Velmurugan , Balachandar Dananjeyan , Ramanathan Babu 
Published: April 2023

Abstract
Background Microorganisms living in insect gut plays a crucial role in the adaptation, growth and development of the insect hosts. The identification and molecular analysis of insect gut microbes will enable us to develop novel strategies for industrial product development, effective utilization of by products and facilitate us with the best pest management practices. In our present study, we employed a culture independent metagenomic approach in conjunction with metaproteomic profiling to enumerate the gut microbes in pure races and cross breeds of silkworm, Bombyx mori L.Results The phylogenetic analysis showed that the gut microflora was majorly grouped into four bacterial classes: Gamma Proteobacteria, Actinobacteria, Cocci and Bacilli. Two novel proteins in both silkworm races were identified and an additional three proteins were found to be differentially expressed between the races.Conclusions Silkworm undergoes radical morphological variations upon metamorphosis and a key challenge in studying gut microflora is the ability to access different genomes and the corresponding protein profiles. The results presented in this study may help to bridge the gap between the unknowns of silkworm gut microbiota and their importance in the silkworm gut ecosystem.
Low expression of Ki-67/MIB-1 labeling index in IDH wild type glioblastoma predicts prolonged survival independently by MGMT methylation status
Authors: Tini Paolo , Yavoroska Mariya , Mazzei Maria Antonietta , Miracco Clelia , Pirtoli Luigi , Tomaciello Miriam , Marampon Francesco , Minniti Giuseppe 
Published: April 2023

Abstract
Purpose The Ki−67/MIB−1 labeling index (LI) is clinically used to differentiate between high and low-grade gliomas, while its prognostic value remains questionable. Glioblastoma (GBM) expressing wild-type isocitrate dehydrogenase IDHwt, a relatively common malignant brain tumor in adults, is characterized by a dismal prognosis. Herein, we have retrospectively investigated the prognostic role of Ki−67/MIB−1-LI in a large group of IDHwt GBMMethods One hundred nineteen IDHwt GBM patients treated with surgery followed by Stupp’s protocol in our Institution between January 2016 and December 2021 were selected. A cut-off value for Ki−67/MIB−1-LI was used with minimal p-value based approach.Results A multivariate analysis showed that Ki−67/MIB−1-LI expression < 15% significantly correlated with a longer overall survival (OS), independently from the age of the patients, Karnofsky performance status scale, extent of surgery and O6-methylguanine (O6-MeG)-DNA methyltransferase promoter methylation status.Conclusions Among others studies focused on Ki−67/MIB−1-LI, this is the first observational study showing a positive correlation between OS of IDHwt GBM patients and Ki−67/MIB−1-LI that we propose as a new predictive marker in this subtype of GBM
Impact of Body Mass Index on postoperative pain and opioïd administration after pressurized intraperitoneal aerosol chemotherapy (PIPAC)
Authors: Tidadini Fatah , Trilling Bertrand , Quesada Jean-Louis , Abba Julio , Foote Alison , Faucheron Jean-Luc , Arvieux Catherine 
Published: April 2023

Abstract
BACKGROUND Intraperitoneal aerosol spray chemotherapy (PIPAC) is a new surgical technique, developed for the treatment of peritoneal carcinomatosis (PC). In this retrospective observational study we assessed the impact of body mass index (BMI) on postoperative pain and opioid consumption.METHODS We analysed pain scores after 100 PIPAC procedures with either oxaliplatin or doxorubicin-cisplatin performed in 49 patients with PC between July 2016 and September 2020. The patients were divided into 4 groups (BMI < 18.5, 18.5 ≥ BMI < 25, 25 ≥ BMI ≤ 29.9 and BMI ≥ 30). Pain was self-rated on a visual analogue scale (VAS) from 0 to 10.RESULTS Univariate logistic regression analysis identified oxaliplatin to be associated with moderate to severe pain (VAS 4–10 at 8h D1) before (OR [95% CI]; 3.75[1.33–10.54]; p = 0.012) and after adjustment (OR [95% CI]; 4.13[1.41–12.11] p = 0.010) on BMI. The level of pain appeared significantly different between the treatment groups (median 2.5[0; 5] vs 0[0; 2.5] p = 0.0017) irrespective of BMI (p = 0.46 and p = 0.226). Multivariate logistic regression analysis identified moderate to severe pain and synchronous PC to be associated with greater use of opioids (OR [95% CI]: 3.91 [1.24–12.32]) and (OR [95% CI]: 5.16 [1.71–15.58]; respectively. Opioids were administered after 45 procedures (45%) and was comparable between the treatment groups. Opioid administration and length-of-stay were similar among BMI bands.CONCLUSION We conclude that BMI is not related to postoperative pain or opioid use and that moderate to severe pain and synchronous PC are factors associated with requiring opioïds.
Neoadjuvant short-course radiotherapy followed by consolidation chemotherapy versus long-course chemoradiotherapy in locally advanced rectal cancer: comparison of overall response rates
Authors: Amariyil Adila , Pathy Sushmita , Sharma Atul , Kumar Sunil , Pramanik Raja , Bhoriwal Sandeep , Pandey RM 
Published: April 2023

Abstract
Background Management of locally advanced rectal cancer is evolving with current emphasis on the addition of chemotherapy to short course radiotherapy regimen. In this study, we primarily aimed to analyse the difference in overall response rates between the experimental regimen of short-course radiotherapy and sequential chemotherapy and standard long-course CTRT in patients of locally advanced rectal cancer.Methods 33 patients of locally advanced rectal cancer were randomized to a standard long-course CTRT arm and SCRT followed by a sequential chemotherapy arm. Patients in the standard arm received 45Gy in 25 fractions over five weeks with concurrent oral capecitabine while patients in the experimental arm received 25Gy in 5 fractions over 1 week followed by 3 cycles of CAPOX chemotherapy. Clinical and radiological response assessment was conducted after the completion of neoadjuvant treatment, right before surgery.Results Radiological tumour regression showed a statistically non-significant trend favouring the experimental arm (rates of complete tumour regression 35.7% versus 23.1%). Pathological tumour regression rates also showed a trend favouring the experimental arm with a pCR rate of 30% versus 20% in the CTRT arm. a higher number of patients in the short-course arm experimental arm experienced grade 3 diarrhoea, whereas acute skin toxicity was seen only in the CTRT arm. Short-course radiotherapy was tolerated with fewer treatment interruptions compared to CTRT.Conclusions Short-course radiotherapy followed by three cycles of CAPOX chemotherapy is comparable to long-course CTRT in terms of tumour response. This is a better alternative regimen with fewer treatment interruptions in a resource-limited setting.
Changes and Comparison of drug resistance surveillance in lower respiratory tract specimens from adult isolates before and during the COVID-19 epidemic
Authors: Yindi Zhou , Gao Dongmei 
Published: April 2023

Abstract
The aim of this paper is to study the changes in the resistance and distribution of pathogenic bacteria isolated from adult lower respiratory tract specimens from our hospital during the novel coronavirus (COVID-19) epidemic.Antimicrobial susceptibility testing is used for adult lower respiratory tract isolates from 2017 to 2022 using automated micro-broth dilution and Kirby-Bauer methods.The top six pathogens isolated in our hospital from 2017 to 2022 were A.baumannii, K.pneumoniae, P.aeruginosa and S.aureus,E.coli and S.maltophilia. The numbers of strains increased from 1041 in 2017 to 1799 in 2022, antibacterial drug resistance rates also changed. There is decline in the number of ESBLs-producing strains.
The rate of isolation of carbapenem-resistant Acinetobacter baumannii (CRAB)decreased to75%、52.1%、61.5%、61.8%、36.8% and 51.6% respectively. Although the number of detected strains of K.pneumoniae increased, the isolation rate of carbapenem-resistant Klebsiella pneumoniae（CRKP）showed a decreasing trend with 12.6%、20.3%、28.3%、17.7%、9.8% and 13.8%, respectively.
The Carbapenem-resistant Pseudomonas aeruginosa（CRPA）also increased from 32 strains in 2017 to 67 strains in 2022，but did not increase isolation rates, The separation rate for 2019 is 26.4% and 17.7% in 2020. The isolation rates of methicillin-resistant Staphylococcus aureus(MRSA) showed a decreasing trend during the COVID-19 epidemic, with 58.1%、41.3%、44.0%、34.1%、32.2% and 31.3%, respectively.During the COVID-19 epidemic, the spread of the pathogenic bacteria within the hospital was interrupted by measures such as reducing outings, wearing masks, nucleic acid testing , sanitary environment disinfection and blocking control. The detection rate of ESBLs, CRKP, CRAB and CRPA in adult lower respiratory tract specimens has been generally decreasing, and the detection rate of MRSA has been gradually decreasing.The rate of CRPA did not increase.
PICLS with human cells - first high-throughput screening method with human assay identifies novel longevity compounds including 2,5-anhydro-D-mannitol that increase chronological lifespan
Authors: Alfatah Mohammad , Zhang Yizhong , Cheng Trishia , Naaz Arshia , Eisenhaber Frank 
Published: April 2023

Abstract
Gerontology research on anti-aging interventions with drugs could be an answer to age-related diseases, aiming at closing the gap between lifespan and healthspan. Here, we present two methods for assaying chronological lifespan in human cells: 1) a version of the classical outgrowth assay with quantitative assessment of surviving cells and 2) a version of the PICLS method (propidium iodide fluorescent-based measurement of cell death). Both methods are fast, simple to conduct, cost-effective, produce quantitative data for further analysis and can be used with diverse human cell lines. Whereas the first method is ideal for validation and testing the post-intervention reproductive potential of surviving cells, the second method has true high-throughput screening potential. The new technologies were validated with known anti-aging compounds (2,5-anhydro-D-mannitol and rapamycin). Using the HTS method, we screened a library of 162 chemical entities and identified three novel anti-aging compounds that extend the cellular longevity of human cells.
Trump, Johnson and New Primitivism - The Manifestations and Causes of Major Crisis in the USA and the UK
Authors: Pribićević Ognjen 
Published: April 2023

Over the past few decades, major changes have taken place in the two most influential Western countries – the USA and the UK. These changes found political expression in Trump’s presidential victory and the triumph of Brexit in 2016. The purpose of this paper is to analyze the changed political reality in these states and explain its causes. In the first part, the author provides a theoretical framework and discusses the old and new categories to provide explanation for the changes in these states. In the second part, he analyzes the main changes in political life in the USA and UK embodied in Donald Trump and British Prime Minister Boris Johnson. In the third part, he points to the basic causes of the crisis in the USA and UK that led to Donald Trump’s victory in 2016 and UK’s withdrawal from the EU.
The countries that were beacons of civilized dialogue among political opponents now harbor growing intolerance, lack of accountability and ruthless mudslinging campaigns against political opponents. The radicalization of political life in the USA culminated in former President Trump’s supporters storming the Congress which left five people dead. In the second most influential state of the Western world, during the pro- remain or pro-leave the EU campaign, a British MP was killed while the politicians were stoking fears of impending wars, genocide and even invoked the politics dating back to Hitler and Napoleon. The policy of exclusiveness pursued by Trump and Brexiteers brought about the social polarization in the USA and the UK unprecedented since the Second World War.
The author proceeds from a hypothesis that deep social and ethnic divisions, rising poverty, less access to higher education for broader social strata, lack of trust in democratic and supranational institutions, obsession with one’s own cultural identity, fear of large-scale immigration and domination of reality culture set the stage for the changes in political life in these countries, making it possible for Trump and Brexit to prevail. The paper provides a critical analysis of previous attempts to account for the victory of Trump and Brexit, particularly the theory of cultural backlash by which US political analysts Norris and Inglehart explain the rise of authoritarian populism in the USA and the UK. The author is of the view that following Biden’s presidential victory in 2020 the situation in the USA will not change significantly since it is not just the politicians that create the political environment but rather the social and economic situation which has deteriorated even further in the wake of the COVID-19 pandemic. The UK has managed to avoid significant radicalization in political life since the divisions over Brexit did not follow partisan and ideological lines as was the case in the USA.
The Ritual-Mythological Semantics of the Azerbaijan Khidir Nabi Holiday
Authors: Albaliyev Shakir Alif oglu 
Published: April 2023

After Novruz holiday the most important holiday according to the ritual-mythological bases and ethno-cultural semantics of Azerbaijani folk holidays is Khidir Nabi holiday. In fact, the holidays of Novruz and Khidir Nabi are not separated from each other as part of the national calendar: these holidays belong to the different stages of the folk calendar and are united according to the ethno-cultural essence and concept. They are the components of the unified mythical system of thinking of people about time, as independent holidays, which do not replace each other and are not alternative. However, it should be noted that Novruz is more widespread due to the ethno-cultural geography than the holiday Khidir Nabi.
The main purpose of the research is to investigate the ritual-mythological semantics of the Azerbaijani Khidir Nabi holiday. The research presented in this paper is mainly based on the historical-comparative and ritual-mythological approach methods.