bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green synthesized silver nanoparticles mediated by Fusarium nygamai isolate AJTYC1: characterizations, antioxidant, antimicrobial, anticancer, and photocatalytic activities and cytogenetic effects
Authors: El-Ansary Abeer E. , Omran Ahmed A. A. , Mohamed Heba I. , El-Mahdy Omima M. 
Published: August 2023

AbstractGreen biosynthesized nanoparticles have a bright future because they can be produced using a method that is more energy-efficient, cost-effective, repeatable, and environmentally friendly than physical or chemical synthesis. In this study, silver nanoparticles (AgNPs) were produced using theFusarium nygamaiisolate AJTYC1. Several techniques were used to characterize the synthesized AgNPs, including UV–Vis spectroscopy, transmission electron microscope, zeta potential analysis, X-ray diffraction analysis, energy dispersive X-ray, and Fourier transform-infrared spectroscopy. AgNPs showed a distinctive surface plasmon resonance (SPR) peak in the UV–visible range at 310 nm. The morphology of the biosynthesized AgNPs was spherical, and the TEM image shows that they ranged in size from 27.3 to 53.1 nm. The notable peaks of the FT-IR results show the different groups for the alkane, alkynes, cyclic alkenes, carboxylic, aromatic amine, esters, and phenolics. Additionally, the results showed that AgNPs had superior antioxidant activity when compared to ascorbic acid and butylated hydroxytoluene, which is a powerful antioxidant. Additionally, AgNPs have antibacterial action utilizing agar diffusion against gram-positive bacteria, gram-negative bacteria, and antifungal activity. AgNPs’ anticancer activity varied depending on the type of cancer it was used to treat, including hepatocellular cancer (HepG2), colorectal carcinoma (HCT116), and breast cancer of the mammary gland (MCF7). The viability of the cancer cell lines was reduced with increasing AgNP concentration. AgNPs also demonstrated promising photocatalytic activity by reducing methylene blue, safranin, crystal violet, and green malachite by 88.3%, 81.5%, 76.4%, and 78.2%, respectively. In addition, AgNPs significantly affected theAllium cepaplant’s mitotic index and resulted in chromosomal abnormalities as compared to the control. Thus, the synthesized AgNPs demonstrated an efficient, eco-friendly, and sustainable method for decolorizing dyes as well as antioxidant, antibacterial, antifungal, and anticancer activities. This could be a huge victory in the fight against numerous dynamic diseases and lessen wastewater dye contamination.
Integrating ecosystem services and life cycle assessment: a framework accounting for local and global (socio-)environmental impacts
Authors: Taelman Sue Ellen , De Luca Peña Laura V. , Préat Nils , Bachmann Till M. , Van der Biest Katrien , Maes Joachim , Dewulf Jo 
Published: August 2023

Abstract
Purpose
Human activities put pressure on our natural ecosystems in various ways, such as globally through the spread of emissions or locally through the degradation of species-rich landscapes. However, life cycle assessment (LCA) studies that integrate ecosystem services (ES) are still in the minority because of intrinsic differences in data, modelling, and interpretation. This study aims to overcome these challenges by developing and testing a framework that comprehensively evaluates the (socio-)environmental impacts of human activities.

Methods
LCA and ecosystem services assessment (ESA) were integrated in two different ways: (1) both methodologies run in parallel and results are combined, and (2) LCA as a driving method where ES are integrated. Because local ESA studies contain the most accurate information but will not be available for all processes in the value chain, it was necessary to advance the life cycle impact assessment method ReCiPe 2016 including three new midpoint impact categories (terrestrial provision, regulation, and cultural ES) and site-generic CFs based on the Ecosystem Services Valuation Database to account for changes in regulating, cultural and provisioning ES due to land use, for the remaining processes in the value chain. Monetary valuation is used to aggregate at the areas of protection (AoP).

Results and discussion
A comprehensive LCA+ES-ESA sustainability assessment framework is developed to account for local and global impacts due to human activities on three AoPs (natural resources, ecosystem quality, and human health and well-being), of which the results are expressed in monetary terms. The framework is able to visualize all benefits and burdens accounted for through the handprint/footprint approach. A simplified terrestrial case study on Scots pinewood shows the applicability of the proposed framework, resulting in a handprint (€2022 9.81E+02) which is four times larger than the footprint (€2022 2.31E+02) for 1 kg of wood produced. Challenges related to the framework such as data availability and database shortcomings (i.e., beyond land use) and ES interrelations are discussed.

Conclusion
While classical LCA studies focus more on burdens, this framework can also take into account benefits, such as the provision of ecosystem services (or the value of the functional unit of the study). Although the integration of both LCA and ESA has been increasingly explored recently, until now no framework has been available that can incorporate results from local ESA, site-specific ESA, and classical LCA studies, which is considered highly relevant to decision-making.
Impact of rolling processes in the production of aluminum packaging assessed through LCA
Authors: Astarita Antonello , De Luca Maria , Sinagra Ciro 
Published: August 2023

Abstract
Purpose
The environmental impact of aluminum cans and aluminum food packaging has been extensively studied in the last years; most of the researchers focused their attention on the whole lifecycle: from the aluminum extraction to the end-of-life management, while there are no dedicated studies devoted to the detailed analysis of the rolling phase. The aim of this paper is to present an LCA analysis focused on the fabrication of the thin plates to be used for can and tray production; the work has been carried out by studying the “Laminazione Sottile” plant with a cradle-to-gate approach, considering as functional unit thin plates of two different alloys.

Materials and methods
Inventory data were collected from the “Laminazione Sottile S.p.A.” plant, and off-plant emissions from external goods and fuels were accounted for based on existent LCA databases. Gabi has been used as software to model the process and calculate the impacts, while the CML method has been used. Most of the data have been directly measured in the plant during 1 year of work.

Results and discussion
The results showed that the most impactful phase is the extraction of aluminum or the use of primary aluminum. Focusing the attention on the manufacturing process, the foundry is the most impacting stage, and the high energy required to melt the aluminum leads to most of the emissions. Moreover, this contribution is probably underestimated in this work because the impacts related to the manufacturing of the facilities used (furnaces and so on) are not considered. Another interesting finding is that both the supply chain and the general consumption have a non-negligible impact, highlighting the necessity to optimize these two aspects by implementing new policies for purchasing and work organization.

Conclusions
The study highlighted that the production of primary aluminum is the most impacting step, followed by the foundry and the hot rolling. Production of thin aluminum plates by rolling requires a lot of energy, so the carbon footprint of these processes is quite high. The chemicals used in the rolling steps play also a role in the general impact of the whole process. The company continuously promotes improvements of the process to reduce the footprint and puts a lot of money and effort to introduce new sensors and technologies to monitor the consumptions and improve the processes.
Ex-ante life cycle assessment of FineFuture flotation technology: case study of Grecian Magnesite
Authors: Eltohamy Hazem , Cecere Giuseppe , Rigamonti Lucia 
Published: August 2023

Abstract
Purpose
This study aims at evaluating the environmental performance of a novel froth flotation technology in mining industry from a life cycle perspective. The technology is being developed under EU Horizon 2020 project titled “FineFuture” (FF) with the aim of saving valuable materials in fine particles that are currently wasted due to lack of technology.

Methods
FF relies on chemically enhancing the physical characteristics of particles allowing it to float and concentrate.
Prospective life cycle assessment (pLCA) was conducted for two possible industrial applications of FF flotation technology in the case study of Grecian Magnesite (GM) which is a main magnesium oxide producer in Europe. Each application can be perceived as a standalone comparative LCA study comparing current system with future system incorporating FF technology on industrial scale.

Results and discussion
The future scenarios did not decisively support FF technology in neither of the two applications from an environmental point of view. When applied to fines of < 4 mm granular size with the aim of material recovery, the future scenario performed better than the current situation only in 5 out of 16 impact categories. The main issue is the added burden of calcination phase. When the technology was tested to upgrade the existing magnesite concentrate before calcination, it introduced some gains in most of the impact categories, but the difference compared to the current situation is not very considerable. Testing improved scenarios showed a great benefit to the overall performance of the scenarios by introducing cleaner fuels and burners in calcination phase.

Conclusion and recommendations
Overall, the results tend to favour applying FF technology to upgrade low quality concentrates rather than beneficiating < 4 mm fines. However, and in any case, if FF technology is to be applied, combining it with cleaner fuels and burners in calcination should be prioritized. Furthermore, it was found that improving the purity (i.e. quality) in the flotation tank output is a key factor from an environmental view. The results also showed little impact of the added electric energy demand from the new units. As any pLCA, the study has limitations mainly originating from the low technology readiness level (TRL) when data collection activities were carried out. Further studies should start from pilot-scale data and adopting more accurate upscaling approaches to calculate the impacts of a full industrial deployment of the technology.
Soil bacterial community composition and function play roles in soil carbon balance in alpine timberline ecosystems
Authors: Yang Yuanyuan , Chen Qianqian , Zhou Yin , Yu Wu , Shi Zhou 
Published: August 2023

Abstract
Purpose
Soil microbial communities and related key ecological processes play critical roles in timberline delineation and soil carbon balance in alpine ecosystems, which are highly vulnerable to climate change. Accordingly, understanding their geographical differentiation will facilitate recognition of ecosystem functions and improve soil carbon models. In this study, we explored the biogeographic patterns of soil bacterial communities and their mechanisms in maintaining soil carbon balance in an alpine timberline ecosystem of the Sygera Mountains, Southeast Tibet.

Materials and methods
Soil samples were collected from typical forest belts above and below the timberline. The abundance and composition of bacterial communities, as well as functional genes, were assessed using the gene chip technology. The relationship of key microbial taxa, functional genes, and soil carbon maintenance was investigated using random forest analysis, multi-model inference, and structural equation modeling.

Results and discussion
The shrubland soil bacterial community exhibited greater diversity compared with the coniferous forest community, with higher Shannon Index and more functional genes at the taxonomic and functional levels, respectively. Bacterial community composition differed between the two forest types, with copiotrophic bacteria more abundant in shrubland, and oligotrophic bacteria more abundant in coniferous forest. The shrubland community was also more efficient at utilizing labile organic carbon, while the coniferous forest community utilized recalcitrant organic carbon more efficiently. Genes related to labile carbon degradation were more intense in shrubland, while genes related to recalcitrant carbon degradation were more concentrated in the coniferous forest. Soil temperature and C:N ratio were dominant drivers of bacterial community composition and function. Besides key soil-environment and microbial properties, certain bacterial taxa and functional genes also exerted unique roles in soil carbon variation.

Conclusions
Significant differences exist in soil bacterial community composition and functions between the two forest types above and below the timberline of the Sygera Mountains. These differences may be attributed to soil temperature and soil C:N ratio. Coupling these microbial variables into the earth system model can improve the predictive power of the carbon feedback process in terrestrial ecosystems.
Robust gradient boosting for generalized additive models for location, scale and shape
Authors: Speller Jan , Staerk Christian , Gude Francisco , Mayr Andreas 
Published: August 2023

AbstractDue to the increasing complexity and dimensionality of data sources, it is favorable that methodological approaches yield robust results so that corrupted observations do not jeopardize overall conclusions. We propose a modelling approach which is robust towards outliers in the response variable for generalized additive models for location, scale and shape (GAMLSS). We extend a recently proposed robustification of the log-likelihood to gradient boosting for GAMLSS, which is based on trimming low log-likelihood values via a log-logistic function to a boundary depending on a robustness constant. We recommend a data-driven choice for the involved robustness constant based on a quantile of the unconditioned response variable and investigate the choice in a simulation study for low- and high-dimensional data situations. The versatile application possibilities of robust gradient boosting for GAMLSS are illustrated via three biomedical examples—including the modelling of thyroid hormone levels, spatial effects for functional magnetic resonance brain imaging and a high-dimensional application with gene expression levels for cancer cell lines.
Status and perspectives of blue economy sectors across the Macaronesian archipelagos
Authors: Fernández-Palacios Yaiza , Kaushik Sadasivam , Abramic Andrej , Cordero-Penín Víctor , García-Mendoza Alejandro , Bilbao-Sieyro Alberto , Pérez-González Yeray , Sepúlveda Pedro , Lopes Isabel , Andrade Carlos , Nogueira Natacha , Carreira Gilberto P. , Magalhães Maria , Haroun Ricardo 
Published: August 2023

AbstractThe global economy is increasingly focusing on the Oceans to meet its needs, for which maritime spatial planning is being fostered to promote this is done sustainably. Thus, assessing the current state and future development trends of the maritime sectors is key to evaluating the performance of the planning process. This is the aim of this study, in which the main maritime sectors related to the blue economy present in the archipelagos of the European Macaronesia Sea Basin, i.e. the Azores and Madeira (Portugal), and the Canary Islands (Spain), were identified and studied. The following maritime sectors were analysed: fisheries, marine aquaculture, marine biotechnology, coastal and maritime tourism, maritime transport, ship repair and maintenance, extraction of aggregates, deep-sea mining, offshore oil and gas, renewable ocean energies, and desalination. As part of the PLASMAR project, partners undertook in 2017–19 a literature review gathering scientific papers, official statistics and reports, which were complemented by interviews with experts from the economic sectors and public administration departments. Results show that while some maritime sectors are well established, others are emerging sectors with varying long-term growth potential. Besides, development patterns vary across the archipelagos. This study represents the first effort in the Macaronesia Sea Basin to set the basis of the current and future development conditions of the blue economy in applicability to maritime spatial planning processes at a regional level. The future post-pandemic context will provide a unique opportunity to promote the blue economy sectors and activities through the support provided by the EU Green Deal and Recovery and Resilience Plans, as well as with the actions envisioned under the United Nations Decade of Ocean Science for Sustainable Development (2021–2030). The European Macaronesia example –given its wide maritime territory, commitment, and capacities in terms of expertise and networking– presents a great potential to act as a good practice to extrapolate the new approach for a sustainable blue economy to other similar geographical settings such as island regions/states.
Is Lipoprotein(a) Clinically Actionable with Today’s Evidence? The Answer is Yes
Authors: Ma Gary S. , Chiou Tommy T. , Wilkinson Michael J. 
Published: August 2023

AbstractPurpose of ReviewLipoprotein(a) is an independent risk factor for cardiovascular disease. We review the ongoing shifts in consensus guidelines for the testing and management of Lp(a) and provide insight into whether current evidence suggests that awareness and testing of Lp(a) is clinically actionable.Recent FindingsGWAS and Mendelian randomization studies have established causal links between elevated Lp(a) and forms of CVD, including CAD and calcific aortic valve disease. Testing of Lp(a) identifies patients with similar risk to that of heterozygous FH, enhances risk stratification in patients with borderline/intermediate risk as determined through traditional factors, and facilitates the assessment of inherited CVD risk through cascade screening in patients with known family history of elevated Lp(a). Reductions in Lp(a) through non-targeted therapies including PCSK9 inhibition and lipoprotein apheresis have demonstrated reductions in ASCVD risk that are likely attributable to lowering Lp(a). Targeted therapies to potently lower Lp(a) are in clinical development.SummaryLp(a) is actionable, and can be used to identify high risk patients for primary prevention and their family members through cascade screening, and to guide intensification of therapy in primary and secondary prevention of ASCVD.
Evaluation of the effect of chemical fertilization and some natural extracts on the production of freesia (Freesia refracta L.) plants: a sustainable approach
Authors: Suedan Eman A. , Abdelmegied Walaa , Hassan Ibrahim A. , EL-kinany Ramy G. 
Published: August 2023

AbstractEffects of different types of fertilizers; traditional NPK (1 g plant−1), 2, 4, 6 and 8 gm L−1 of moringa leaves extract (MLE), 10, 20, 40 and 60 mL L−1 of aloe leaves extract (AL) on vegetative growth, flowering Corms production and chemical constituents of Freesia refracta L. were evaluated during two successive seasons (2020–2021 and 2021–2022). Treating plants with MLE caused increases in plant height, number if leaves and number of flowers by 35.2%, 8.2% and 16.3%, respectively, while treating plants with Al caused increases by 33.2%, 7.9% and 15.4% in the same parameters, respectively, averaged between different concentrations and growth seasons. The results revealed that, treating plants with 8 gm L−1 MLE caused a significant increase in the vegetative growth, flowering, and corms production. These improvement fits with the many goals of the 17 United Nation Sustainable Goals (UNSDGs); especially SDG2 (Zero hunger), SDG13 (climate action) and SDG15 (Life on land). Moreover, the chemical composition of plant was improved significantly.
The Severity of Direct Antiglobulin Test Negative ABO Hemolytic Disease of Newborn: A Retrospective Analysis at a Tertiary Children’s Hospital
Authors: Ma Huimin , Sheng Zhe , Xu Jin 
Published: August 2023

AbstractThis study aimed to evaluate the severity of ABO hemolytic disease of newborn (ABO-HDN) with negative direct antiglobulin test (DAT), which was identified by elution test. We retrospectively reviewed the clinical records of all neonates admitted with the diagnosis of neonatal hyperbilirubinemia requiring phototherapy or exchange transfusion. Neonates were divided into four groups according to their immunohematology test results. Then their essential laboratory results, magnetic resonance image (MRI), brainstem auditory evoked potential (BAEP) findings, and rate of exchange transfusion were compared between different groups. We found that neonates in ABO-HDN with negative DAT group developed jaundice faster and anaemia more severely than those in the non-HDN group. Although they might get less severe anaemia than neonates in ABO-HDN with positive DAT group and the Rh-HDN group, neonates in ABO HDN with negative DAT group might develop jaundice as quickly as the latter two groups. As to MRI and BAEP findings, there were no significant differences among the four groups. The rate of exchange transfusion in ABO-HDN with negative DAT group was higher than that in the non-HDN group but lower than that in ABO-HDN with positive DAT group, though without statistical significance. It suggested that in the presence of clinical suspicion of ABO-HDN with negative DAT result, the elution test should be added to rule out or confirm the diagnosis to help prevent the morbidity from hyperbilirubinemia.
Understanding the provenance and production process of historic mortars—a novel approach employing calcareous nannofossils
Authors: Falkenberg Janina , Kaplan Ulrich , Mutterlose Joerg 
Published: August 2023

AbstractLimestones (CaCO3) have been an important source for masonry and mortars throughout approx. 10,000 years of human history. They are often composed of calcitic shells of minute marine algae, known as calcareous nannofossils. The 0.25–30-µm large calcitic skeletons of these primary producers have been well documented from various archaeological materials including building stones of masonry. Surprisingly, these tiny microfossils were recently also observed in medieval mortars and mortar-based materials, even though the carbonate-based source rocks of the mortars have been heated in kilns for quicklime production. Burning experiments of carbonate-rich sedimentary rocks, containing well-preserved and abundant calcareous nannofossils, documented a deteriorating preservation and a decrease of diversity and relative abundance of the nannofossils with increasing temperatures.Alongside the lime-based binder historic mortars often contain under- and overburnt lime lumps and carbonate-rich aggregates; the latter were added after the heating process. Lime lumps and aggregates offer additional, so far not yet fully understood information on the burning process. Here, calcareous nannofossils were studied in ultra-thin sections of historic mortars, resulting in the separate analysis of the binder, lime lumps and aggregates. The findings allow (i) a more precise provenance of the limestones and (ii) a more accurate reconstruction of the temperatures reached during historic quicklime production. Our study thus improves the provenance analysis approach of limestones and sheds light on historic technology of mortar production by using calcareous nannofossils.