bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional evaluation of cyclosporine metabolism by CYP3A4 variants and potential drug interactions
Authors: Kong Qihui , Gao Nanyong , Wang Yahui , Hu Guoxin , Qian Jianchang , Chen Bingbing 
Published: January 2023

The aim of this study is to investigate the effects of CYP3A4 genetic polymorphisms on the metabolism of cyclosporine (CsA) in vitro and identify drugs that interact with CsA. An enzymatic incubation system was developed to evaluate the kinetic parameters of CYP3A4 on CsA catalysis. A total of 132 drugs were screened to identify potential drug–drug interactions. Sprague–Dawley rats were used to determine the interaction between CsA and nimodipine and nisoldipine. The metabolite AM1 was measured by ultra-performance liquid chromatography–tandem mass spectrometry. The results demonstrate that 16 CYP3A4 variants (CYP3A4.7, 8, 9, 12, 13, 14, 16, 18, 19, 23, 24, 28, 31, 32, 33, and 34) have a lower metabolic capacity for CsA, ranging from 7.19% to 72.10%, than CYP3A4.1. In contrast, the relative clearance rate of CYP3A4.5 is significantly higher than that of CYP3A4.1. Moreover, CYP3A4.20 loses its catalytic ability, and five other variants have no significant difference. A total of 12 drugs, especially calcium channel blockers, were found to remarkably inhibit the metabolism of CsA with an inhibitory rate of over 80%. Nimodipine inhibits the activity of CsA in rat liver microsomes with an IC50 of 20.54 ± 0.93 μM, while nisoldipine has an IC50 of 16.16 ± 0.78 μM. In in vivo, three groups of Sprague–Dawley rats were administered CsA with or without nimodipine or nisoldipine; the AUC(0-t) and AUC(0-∞) of CsA were significantly increased in the nimodipine group but not obviously in the nisoldipine group. Mechanistically, the inhibition mode of nimodipine on cyclosporine metabolism is a mixed inhibition. Our data show that gene polymorphisms of CYP3A4 and nimodipine remarkably affect the metabolism of CsA, thus providing a reference for the precise administration of CsA.
Extracellular vesicles derived from host and gut microbiota as promising nanocarriers for targeted therapy in osteoporosis and osteoarthritis
Authors: Cheung Kenneth Chat Pan , Jiao Ma , Xingxuan Chen , Wei Jia 
Published: January 2023

Osteoporosis (OP), a systemic bone disease that causes structural bone loss and bone mass loss, is often associated with fragility fractures. Extracellular vesicles (EVs) generated by mammalian and gut bacteria have recently been identified as important mediators in the intercellular signaling pathway that may play a crucial role in microbiota-host communication. EVs are tiny membrane-bound vesicles, which range in size from 20 to 400 nm. They carry a variety of biologically active substances across intra- and intercellular space. These EVs have developed as a promising research area for the treatment of OP because of their nanosized architecture, enhanced biocompatibility, reduced toxicity, drug loading capacity, ease of customization, and industrialization. This review describes the latest development of EVs derived from mammals and bacteria, including their internalization, isolation, biogenesis, classifications, topologies, and compositions. Additionally, breakthroughs in chemical sciences and the distinctive biological features of bacterial extracellular vesicles (BEVs) allow for the customization of modified BEVs for the therapy of OP. In conclusion, we give a thorough and in-depth summary of the main difficulties and potential future of EVs in the treatment of OP, as well as highlight innovative uses and choices for the treatment of osteoarthritis (OA).
Identification of therapeutic targets for osteosarcoma by integrating single-cell RNA sequencing and network pharmacology
Authors: Wang Yan , Qin Di , Gao Yiyao , Zhang Yunxin , Liu Yao , Huang Lihong 
Published: January 2023

Background: Osteosarcoma (OS) is a common primary tumor with extensive heterogeneity. In this study, we used single-cell RNA sequencing (scRNA-seq) and network pharmacology to analyze effective targets for Osteosarcoma treatment.Methods: The cell heterogeneity of the Osteosarcoma single-cell dataset GSE162454 was analyzed using the Seurat package. The bulk-RNA transcriptome dataset GSE36001 was downloaded and analyzed using the CIBERSORT algorithm. The key targets for OS therapy were determined using Pearson’s correlation analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed on key targets. The DeepDR algorithm was used to predict potential drugs for Osteosarcoma treatment. Molecular docking analysis was performed to verify the binding abilities of the predicted drugs and key targets. qRT-PCR assay was used to detect the expression of key targets in osteoblasts and OS cells.Results: A total of 21 cell clusters were obtained based on the GSE162454 dataset, which were labeled as eight cell types by marker gene tagging. Four cell types (B cells, cancer-associated fibroblasts (CAFs), endothelial cells, and plasmocytes) were identified in Osteosarcoma and normal tissues, based on differences in cell abundance. In total, 17 key targets were identified by Pearson’s correlation analysis. GO and KEGG analysis showed that these 17 genes were associated with immune regulation pathways. Molecular docking analysis showed that RUNX2, OMD, and CD4 all bound well to vincristine, dexamethasone, and vinblastine. The expression of CD4, OMD, and JUN was decreased in Osteosarcoma cells compared with osteoblasts, whereas RUNX2 and COL9A3 expression was increased.Conclusion: We identified five key targets (CD4, RUNX2, OMD, COL9A3, and JUN) that are associated with Osteosarcoma progression. Vincristine, dexamethasone, and vinblastine may form a promising drug–target pair with RUNX2, OMD, and CD4 for Osteosarcoma treatment.
Establishment of a novel experimental system for studying the photoperiodic response of short-day dicots using soybean ‘cotyledon-only plant’ as material
Authors: Zhang Chunlei , Xu Xin , Chen Fulu , Yuan Shan , Wu Tingting , Jiang Bingjun , Sapey Enoch , Wu Cunxiang , Sun Shi , Guo Changhong , Han Tianfu 
Published: January 2023

Soybean is an important model crop for photoperiodic response studies in plants and contributes significantly to the study of plant development and physiology in the past century. Because soybean plant is much bigger in size and longer in life cycle than Arabidopsis, it needs much more space for growth and time for investigation, which significantly hamper the efficiency of research. In the current study, we tested the photoperiodic response of a distinctive artificially-made cotyledon-only plant (COP) using a photoperiod-sensitive soybean variety Zigongdongdou (ZGDD) and other varieties with diverse sensitivity to photoperiod. ZGDD COPs flowered 39.4 ± 2.5 d after emergence under short-day conditions but maintained vegetative growth under long-day and night break conditions, which is similar to the case in the intact ZGDD plants. The COPs of early-maturing and medium-maturing soybean varieties also grew and flowered normally under natural day-length conditions. At the molecular level, the key genes in the photoperiodic pathway such as E1, GmFT1a, GmFT2a, and GmFT5a in the COPs also showed the same photoperiod sensitivity as in the intact plants. In addition, a simpler material of COP with only one cotyledon and root was generated and found to be sensitive to photoperiod as well. Notably, the COPs are only one-fifth the height of intact plants and one-third the maximum diameter of the intact plants grown in chambers 30 d after emergence. Based on COPs, we established a novel experimental system characterized by an entire photoperiodic response and longer longevity of cotyledons in addition to small plant size, ensuring the consistency, reliability, and stability of plant materials. COPs have the potential to be a novel model material for studies of the developmental biology of soybean and other dicots.
Aromatherapy with inhalation effectively alleviates the test anxiety of college students: A meta-analysis
Authors: Luan Jiage , Yang Mengshu , Zhao Yan , Zang Yu , Zhang Ziyu , Chen Haiying 
Published: January 2023

ObjectiveTest anxiety is one of the common psychological and behavioral problems of college students, which can result in poor academic performance and even academic failure. Aromatherapy has been proposed as a promising method to reduce test anxiety in college students, but its precise efficacy has not been fully confirmed. This meta-analysis evaluated the effects of aromatherapy on the symptoms of test anxiety in college students to serve as a reference for future research and provide more scientific and exact evidence.MethodsPubMed, The Cochrane Library, Web of Science, Embase, CINAHL, Science Direct, Chinese National Knowledge Infrastructure (CNKI), Chinese Science and Technology Journal Full-Text Database (VIP), and Wanfang Data were electronically searched from inception to June 2022 to identify randomized controlled trials (RCTs) on aromatherapy for treating students’ test anxiety. The Cochrane Risk of Bias Assessment Tool for RCTs was used by two reviewers to critically and independently assess the methodological quality of the included studies. Review Manager 5.4 was used for the meta-analysis. Stata 17.0 was used for sensitivity analysis and Egger’s test.ResultsSeven RCTs included 425 patients, with a moderate risk of bias in the included studies. The meta-analysis found that aromatherapy effectively reduced test anxiety in college students (SMD = −0.67, p &lt; 0.01), with high heterogeneity of results (I2 = 72%). The different types of essential oil devices used in the study are the source of inter-study heterogeneity. Subgroup analysis suggested that most effective methods were aromatherapy with compound essential oils (SMD = −0.91, p &lt; 0.01), and using cloths or pads as the essential oil devices (SMD = −1.03, p &lt; 0.01). There was no difference between aromatherapy and placebo control groups (SMD = −0.65, p = 0.25).ConclusionAromatherapy with inhalation can help relieve test anxiety in college students, but more and higher-quality studies are needed. This study provides a reference for future research designs in terms of the selection of essential oil types and devices and the design of research groups.Systematic review registration[https://www.crd.york.ac.uk/prospero/], identifier [CRD42022345767].
Party branches, policy perception and corporate social responsibility: Evidence from Chinese private enterprises
Authors: Yao Zhenjiu , Zhang Zengtian , Ma Jun 
Published: January 2023

IntroductionParty branches embedded in private enterprises are a unique phenomenon in the Chinese economy, but few studies have focused on the economic consequences. We hope to explore the impact of party branches on small and medium-sized private enterprises’ corporate social responsibility (CSR) in China in order to fill the gaps in current research.MethodsData were used from the 11th Chinese Private Enterprise Survey (CPES) in 2014. The study uses the methodology of fixed effect model, mediation analysis and moderation analysis. Moreover, propensity score matching and Heckman two-step method deal with the endogeneity problem and check the robustness of the results.ResultsWe find that, first, the embedding of party branches will improve the CSR performance of private enterprises in various dimensions by enhancing the perception of private enterprises in policy; second, in various influence pathways, the party branches will enhance the perceptions of policy related to economic interests, which has a more significant impact on enhancing the performance of philanthropic CSR. Further research reveals that business owners’ first-given and later-generated political connections support the party branches’ perception of policies related to economic and social interests, respectively.DiscussionThe findings suggest that political intervention by China’s ruling party in private firms promotes CSR performance, but this is influenced by the political connections of the firm owners. In addition to providing empirical support for the study of corporate behavior in the Chinese context, this paper identifies the impact and development trajectory of the party branches of China’s ruling party on CSR. However, this paper does not discuss the implicit costs required for party branches to promote CSR, and we hope that future research will make further explorations.
The construction and practice path of safety education mechanism in colleges and universities integrating the psychological characteristics of students in the new era
Authors: Li Honglin 
Published: January 2023

BackgroundWith the rapid development of higher education in China, the scale of colleges and universities is expanding, and the phenomenon of campus socialization is becoming more and more obvious. In particular, the campus and its surrounding environment are becoming more and more complex, which brings many hidden dangers in university life.ObjectiveIn order to improve the effectiveness of safety education in colleges and universities and maintain the long-term effectiveness of college students’ safety awareness, the paper proposes the construction and practice path of college safety education mechanism that integrates the psychological characteristics of students in the new era.MethodsSecurity issues facing universities at home, this track identifies the relationship between campus security incidents and security education and advocacy. Eight solutions to prevent and reduce incidents in schools. The paper proposes to give importance to the study of the security of college students, to create an awareness of security questions in the bank based on the recommendation algorithm, and to create to have online learning and testing for safety awareness.ResultsThe passing rate of 10 majors such as humanities, composition and theory of composition technology was 100%, accounting for 12% of the 83 enrolled majors, and the passing rate of 54 majors such as clinical medicine was over 90%.ConclusionThe safety online learning and testing system of college students’ safety education is lively in form and highly accepted by students. The development of college students’ safety education starts from the time of receiving the university admission notice, making full use of the “golden time,” so as to effectively prevent and reduce the occurrence of campus safety accidents.
Person-centered evaluation of positive body image among Chinese nursing students: Associations with body appreciation, self-concept clarity, and negative affect
Authors: Zhang Ting , Su Dan , Li Shuwen , Yang Yajuan , Li Huiping 
Published: January 2023

BackgroundNowadays, the effect of body image (BI) on negative affect among nursing college students has been ignored. This study used person-centered methods to evaluate the profiles of body appreciation (BA) among Chinese nursing students. We explored the predictive factors of these profiles and examined the difference in negative affect among the profiles.MethodsA cross-sectional survey study was conducted in a university in Anhui province, China. Five hundred and forty-one Chinese nursing students from a nursing college of a larger university completed this survey. Participants completed the Sociodemographic Characteristics Questionnaire, the Self-concept Clarity Scale, the Body Appreciation Scale-2, and the Depression Anxiety Stress Scale. Latent profile analysis was employed to classify the BA profiles, and multinomial logistic regression analysis were conducted using the three-step regression and Bolck-Croon- Hagenaars method to test the relationship between the latent profiles of BA, several covariates (including gender, age, year of schooling, residence, academic achievement level, interpersonal relationship status, whether the student is an only child, duration of use of social media platforms, body mass index (BMI), and self-concept clarity (SCC)), and negative affect.ResultsAnalysis resulted in a four-profile model that included a “low BA” group (14.42%), an “upper medium BA” group (36.41%), a “lower medium BA” group (36.97%), and a “high BA” group (12.20%). SCC, years of schooling, interpersonal relationship status, and BMI were found to be the predictive factors of BA profiles. The four profiles differed in levels of stress, depression, and anxiety.ConclusionOur study showed that more than half of nursing students were dissatisfied with their BI and the predictive factors evaluated and negative affect were associated with the profiles of BA. Nursing faculties should take measures to help students develop a more appropriate awareness of nursing aesthetics, a healthier lifestyle, a better interpersonal relationship with family members and peers, and a clearer self-concept to promote body appreciation and reduce negative affect related to BI.
Mental disorders are no predictors to determine the duration of cannabis-based treatment for chronic pain
Authors: Rometsch Caroline , Ott Stephan , Festl-Wietek Teresa , Jurjut Anna-Maria , Schlisio Barbara , Zipfel Stephan , Stengel Andreas , Herrmann-Werner Anne 
Published: January 2023

BackgroundChronic pain (CP), a complex biopsychosocial disorder with a global prevalence of up to 33%, can be treated by following multidisciplinary approaches that may include cannabis-based medicine (CBM). However, because CBM continues to be a new treatment, questions remain regarding the ideal duration for CBM and its psychosocial determinants, including mental comorbidities.MethodsIn a retrospective cross-sectional study involving 46 patients with CP (ICD-10 code F45.4-), three validated instruments—the German Pain Questionnaire, the Depression Anxiety Stress Scale (DASS), and the Marburg Questionnaire of Habitual WellBeing—were used to identify pain-specific psychosocial determinants and mental disorders. Descriptive analyses, a group differences analysis, and a logistic regression analysis were performed using SPSS.ResultsThe patients most frequently reported low back pain as the primary location of their CP, and in attributing the condition to tissue damage, most had largely adopted a somatic orientation in conceptualizing their illness. Most had experienced CP for more than 5 years (M = 5.13 years, SD = 1.41) and, as a consequence, faced significant restrictions in their everyday life and exhibited low subjective wellbeing (MFHW median = 4.00, N = 43, Q1: 2.00, Q3: 9.00, range: 0–20). Comorbidities among the patients included depression, (DASS-Depression, median: 11.50, Q1: 7.00, Q3: 16.25), anxiety (DASS-Anxiety, median: 4.50, Q1: 2.75, Q3: 8.00), and stress (DASS-Stress, median: 11.00, Q1: 7.00, Q3: 15.00). Between the two cannabis-based treatments with a course lasting either less or more than a year, the duration of treatment showed no between-group differences in terms of sociodemographic factors, pain-specific factors, conceptualizations of the illness, or mental disorders. Psychosocial determinants such as subjective wellbeing and mental comorbidities were not significant predictors of the duration of cannabis-based treatment.ConclusionWe found no evidence indicating that the benefits of short-term vs. long-term cannabis-based treatment can be predicted by mental comorbidities or psychosocial factors. However, because CBM may be included in approaches to treat CP, questions about the ideal duration of such treatment remain to be answered.
Key issues of health and safety for workers in residential aged care: An expert study
Authors: Seaward Liz , Morgan Damian , Thomson Alana 
Published: January 2023

IntroductionResidential aged care (RAC) represents a fast-growing sector within Australia's health care system and is characterized by high levels of workplace injury. To better understand this injury problem, this study investigated key informant perspectives concerning sector occupational health and safety (OHS) focused on key issues associated with the risk of worker injury.MethodSemi-structured interviews were undertaken with nine key informants representing (OHS) specialists, healthcare employers, regulators, worker association representatives, and academic researchers in OHS or healthcare. Interviews were transcribed verbatim and analyzed using thematic analysis.ResultsThis study identified six themes on OHS within RAC including (i) the physical and emotional nature of the work, (ii) casualization of employment, (iii) prioritization, (iv) workforce profile, (v) OHS role construction, and (vi) clinical standards. The study highlighted differences in OHS roles between RAC and other safety-critical sectors regarding governance and management of OHS. The key informants identified a propensity within RAC to downplay or disregard worker OHS issues justified through prioritizing resident safety. Further, neither OHS professional nor institutional logics are prominent in RAC leadership and decision-making where the emphasis is placed on mandatory standards to maintain funding purposes. Several recommendations are made to address identified issues.
The indirect effects of food insecurity on obesogenic environments
Authors: Allen Jeffrey 
Published: January 2023

IntroductionThe Centers for Disease Control and Prevention (CDC) estimates 39.8% of United States (US) residents have obesity. This study examined obesity-related factors at the county-level to determine the indirect effects on physical inactivity, insufficient sleep duration, income inequality, food insecurity, on obesity rates.MethodologyUsing the 2018 Robert Wood Johnson Foundation (RWJF) County Health Rankings data set, a multiple regression analysis was conducted to measure the percentage of the obesity rate explained by physical inactivity, insufficient sleep duration, food insecurity, and income inequality via geographically weighted county means. RWJF combines US federal and state datasets to produce a composite dataset comprised of information primarily from adults over the age of 18 from the 3,143 counties found within US borders. The aggregate county-level data serves as the unit of measure (N = 3,143). The indirect relationships (the product of two direct relationships) between obesity-related variables and obesity were measured and illustrated through a path analysis model.ResultsThis study found the combination of independent variables explained 53% of the obesity rates in the US, R2 = 0.53, p &lt; 0.001, two-tailed. This study also found that food insecurity has both a direct and indirect effect on obesity, physical inactivity, and insufficient sleep duration. Physical inactivity has a direct effect on obesity and insufficient sleep duration, along with an indirect effect on obesity. Insufficient sleep duration has a direct effect on obesity.ConclusionThis analysis found that food insecurity indirectly impacts an obesogenic environment and drives county-level BMI averages. The dataset used for analysis predates the COVID-19 pandemic but presents the effect of food insecurity during a normative year. The findings, though interesting, provide an opportunity for future research.