bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro oogenesis from murine premeiotic germ cells using a new three-dimensional culture system
Authors: Wang Lu , Yan Zi-Hui , He Tao-Ran , Liu Hai-Xia , Li Yu-Kang , Niu Yi-Lin , Wang Jun-Jie , De Felici Massimo , Ge Wei , Shen Wei 
Published: July 2023

AbstractA faithful reconstitution of the complete process of oogenesis in vitro is helpful for understanding the molecular mechanisms, genetics, and epigenetic changes related to gametogenesis; it can also be useful for clinical drug screening, disease research, and regenerative medicine. To this end, given the consensus that murine female germ cells initiate meiosis at E13.5, substantial works have reported the successful generation of fertile oocytes using E12.5 female gonads as starting materials. Nevertheless, our data demonstrated that murine germ cells at E12.5 have heterogeneously initiated a meiotic transcriptional program based on a measurement of pre‐mRNAs (unspliced) and mature mRNAs (spliced) at a single-cell level. Therefore, to establish a platform that faithfully recapitulates the entire process in vitro (from premeiotic murine germ cells to fully developed oocytes), we here report a novel three-dimensional organoid culture (3-DOC) system, which successfully induced fully developed oocytes from E11.5 premeiotic female germ cells (oogonia). Compared with 2D culture and other 3D culture methods, this new culture system is more cost-effective and can create high-quality oocytes similar to in vivo oocytes. In summary, our new culture platform provides an experimental model for future research in regenerative medicine and reproductive biology.
Scaling-up and future sustainability of a national reproductive genetic carrier screening program
Authors: Fehlberg Zoe , Best Stephanie , Long Janet C. , Theodorou Tahlia , Pope Catherine , Hibbert Peter , Williams Sharon , Freeman Lucinda , Righetti Sarah , Archibald Alison D. , Braithwaite Jeffrey 
Published: July 2023

AbstractAn understanding of factors influencing implementation is essential to realise the benefits of population-based reproductive genetic carrier screening programs. The aim of this study was to synthesise data collected during the Australian Reproductive Genetic Carrier Screening Project (Mackenzie’s Mission) to track how priorities shifted over time and identify important factors during scaling-up and for sustainment. We used a multi-method qualitative approach to integrate longitudinal project data collected from 10 project committees with 16 semi-structured interviews conducted with study team members. Both datasets were analysed using the Consolidated Framework for Implementation Research (CFIR) to identify constructs of interest within early, mid-point, and future implementation phases. Several CFIR constructs were present across implementation. The complexity of implementation presented challenges that were overcome through a quality-designed and packaged product, formal and informal networks and communication, and access to knowledge and information. Addressing the diverse consumer needs through resources and increasing community and non-genetic speciality engagement remained a priority throughout and for future sustainment. Going forward, further addressing program complexities and securing funding were emphasised. By applying an implementation framework, findings from this study may be useful for future effort towards building and/or sustaining reproductive genetic carrier screening programs.
Facile morphological tuning of thin film composite membranes for enhanced desalination performance
Authors: Mohammed Shabin , Aburabie Jamaliah , Hashaikeh Raed 
Published: July 2023

AbstractPolyamide (PA) membranes with a thin selective layer have been widely investigated for desalination and water treatment. Several modifications have been proposed over the years to tailor the morphology of such thin film composite (TFC) membranes by altering the support and/or selective layers to achieve superior performance. In this study, a facile approach towards fabricating a highly wrinkled selective layer has been demonstrated through bio-inspired modification of the support layer with Y-type zeolites. Results showed that incorporating zeolites in a smaller dimension (200 nm) produced by a unique ball milling technique is favorable for a defect-free selective layer in comparison to larger commercial zeolites. PA membranes formed by the interfacial polymerization (IP) of Piperazine (PIP) and 1,3,5-Benzenetricarbonyl trichloride (TMC) revealed highly wrinkled morphology due to the presence of zeolites in the TFC interlayer. At optimum fabrication conditions, the membrane exhibited a fast transport of 22.5 ± 2.2 Lm-2h-1bar-1 with a salt rejection of 48.6, 91.3, 99.1, and 99.5% for NaCl, MgCl2, MgSO4, and Na2SO4, respectively. Besides the unique preparation of zeolites in smaller dimensions, the novelty of this study lies in the facile membrane pretreatment before IP to achieve wrinkled PA membranes for enhanced nanofiltration performance.
Landing site of the Chang’e-6 lunar farside sample return mission from the Apollo basin
Authors: Zeng Xingguo , Liu Dawei , Chen Yuan , Zhou Qin , Ren Xin , Zhang Zhoubin , Yan Wei , Chen Wangli , Wang Qiong , Deng Xiangjin , Hu Hao , Liu Jianjun , Zuo Wei , Head James W. , Li Chunlai 
Published: July 2023

AbstractTo address questions about the multiple lunar nearside–farside dichotomies and to provide new insights into both the early impact history of the Solar System and the geological evolution of the Moon, the Chang’e-6 (CE-6) landing zone has been selected to lie within the lunar farside South Pole–Aitken (SPA) basin in the southern part of the Apollo basin (150–158° W, 41–45° S), a site that provides access to a diversity of SPA material. Here, we describe the geomorphology, geology and chronology of three candidate sampling sites within this zone that are likely to ensure safe landing and sampling. The geological characteristics indicate that CE-6 is expected to collect lunar farside SPA ejecta fragments, possible mantle material and young (roughly 2.40 Gyr-year-old) and/or old (roughly 3.43 Gyr-year-old) basaltic material, all of which will provide important guidance for future in situ farside sample collection and deepen our understanding of the evolution of the Moon.
NF-κB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells
Authors: Zhao Mingming , Chauhan Prashant , Sherman Cheryl A. , Singh Amit , Kaileh Mary , Mazan-Mamczarz Krystyna , Ji Hongkai , Joy Jaimy , Nandi Satabdi , De Supriyo , Zhang Yongqing , Fan Jinshui , Becker Kevin G. , Loke Png , Zhou Weiqiang , Sen Ranjan 
Published: July 2023

AbstractThe nuclear factor kappa B (NF-κB) family of transcription factors orchestrates signal-induced gene expression in diverse cell types. Cellular responses to NF-κB activation are regulated at the level of cell and signal specificity, as well as differential use of family members (subunit specificity). Here we used time-dependent multi-omics to investigate the selective functions of Rel and RelA, two closely related NF-κB proteins, in primary B lymphocytes activated via the B cell receptor. Despite large numbers of shared binding sites genome wide, Rel and RelA directed kinetically distinct cascades of gene expression in activated B cells. Single-cell RNA sequencing revealed marked heterogeneity of Rel- and RelA-specific responses, and sequential binding of these factors was not a major mechanism of protracted transcription. Moreover, nuclear co-expression of Rel and RelA led to functional antagonism between the factors. By rigorously identifying the target genes of each NF-κB subunit, these studies provide insights into exclusive functions of Rel and RelA in immunity and cancer.
Detecting the interaction between urban elements evolution with population dynamics model
Authors: Jin Min , Wang Lizhe , Ge Fudong , Yan Jining 
Published: July 2023

AbstractExploring the evolution of urban elements can improve understanding of the developmental process of city and drive such development into a better direction. However, the non-linearity and complexity of changes in urban elements have brought great challenges to understanding this process. In this paper, we propose a cross-diffusion partial differential equation based on ecological dynamics to simulate the evolutionary process of urban elements from the microscopic viewpoint. The interaction between urban elements is simulated by constructing a non-linear and spatiotemporal change equation, and the main influence between elements is evaluated by the key parameters in the discussed equation. Our model is first experimented to time-series data on population density and housing prices to analyzes the interaction of these two elements in the evolution process. We then extend the model to label data, land cover data, to obtain a quantitative expression of the interaction between different land types in the process of urban land cover change.
Long non-coding RNA SOX2-OT enhances cancer biological traits via sponging to tumor suppressor miR-122-3p and miR-194-5p in non-small cell lung carcinoma
Authors: Dodangeh Fatemeh , Sadeghi Zahra , Maleki Parichehr , Raheb Jamshid 
Published: July 2023

AbstractThe oncogenic role of long non-coding RNA SOX2 overlapping transcript (SOX2-OT) has been demonstrated as a miRNA decay system that sponges tumor suppressor miRNA, including miR-122-3p in glioblastoma and miR-194-5p in glioblastoma, gastric, and colorectal cancers. However, the molecular function of SOX2-OT remains unknown in most cancers, including lung cancer. In the current study, we aimed to evaluate the downstream regulatory function of SOX2-OT in A549 and Calu-3 lung cancer cell lines. We knocked down SOX2-OT expression using an RNA interference system, which significantly decreased expression in A549 and Calu-3 cells. The expression of down-regulating miRNAs (miR-122-3p and miR-194-5p) was evaluated, revealing increased expression of miR-122-3p and miR-194-5p. Additionally, the expression of miRNAs downstream mRNA, including FOXO1 (Forkhead Box O1) and FOXA1 (Forkhead Box O1), changed. Recently, critical roles of FOXO1 and FOXA1 proteins in pathways involved in proliferation, metastasis and apoptosis have been demonstrated. Downstream changes in cellular traits were assessed using MTT, flow cytometry, metastasis and apoptosis assays. These assessments confirmed that the biological behaviors of lung cancer cells were influenced after SOX2-OT knockdown. In summary, the present study highlights the oncogenic role of SOX2-OT through the regulation of miR-122-3p/FOXO1 and miR-194-5p/FOXA1 pathways.
Influence of α-Fe2O3, CuO and GO 2D nano-fillers on the structure, physical properties and antifungal activity of Na-CMC–PAAm blend
Authors: Abou Elfadl A. , Ibrahim Asmaa M. M. , El Sayed Adel M. , Saber S. , Elnaggar Sameh , Ibrahim Ibrahim M. 
Published: July 2023

AbstractThe present work aims to improve the uses of the carboxymethyl cellulose–polyacrylamide (Na-CMC–PAAm) blend for energy storage, optoelectronic applications, biological control, and plant disease management. Nano-sized materials (α-Fe2O3 nanoplates (NP), CuO NP, and GO nanosheets (NS), were synthesized and incorporated into the blend. The phase purity and morphologies of the used fillers were studied by XRD and HR-TEM. The interactions and complexation between the nano-fillers and the blend chains were investigated using XRD and FTIR spectra. The chemical composition and surface morphology of the nanocomposites were studied using EDS and FE-SEM analysis. UV-vis-NIR spectra revealed that the blend shows about 95% transmittance, reduced by 10–30% after doping. The absorption and refractive indices, as well as the optical gaps of the blend, were greatly affected by the doping. The dielectric constant and loss depend on the type of filler and the applied frequency. The maximum ac conductivity of the blend at 303 K and 4.0 MHz is 21.5 × 10–4 S/m and increased to 23.5 × 10–4 S/m after doping with CuO NP. The thermal stability, activation energy, stress–strain curves, and tensile strength are dependent on the filler type. All nanocomposite solutions except the blend exhibited a wide range of antifungal properties against pre- and post-harvest phytopathogenic fungi. Aspergillus niger among the examined fungi showed high sensitivity to the tested nanocomposite solutions. Furthermore, the CuO/blend nanocomposite had the highest antifungal activity against all tested fungi. Based on that, we suggest the use of CuO/blend and GO/blend nanocomposites to control and combat pre- and post-harvest fungal plant diseases.
Effects of basin-scale climate modes and upwelling on nearshore marine heatwaves and cold spells in the California Current
Authors: Dalsin Michael , Walter Ryan K. , Mazzini Piero L. F. 
Published: July 2023

AbstractMarine heatwaves and cold spells (MHWs/MCSs) have been observed to be increasing globally in frequency and intensity based on satellite remote sensing and continue to pose a major threat to marine ecosystems worldwide. Despite this, there are limited in-situ based observational studies in the very shallow nearshore region, particularly in Eastern Boundary Current Upwelling Systems (EBUS). We analyzed a unique dataset collected in shallow waters along central California spanning more than four decades (1978–2020) and assessed links with basin-scale climate modes [Pacific Decadal Oscillation (PDO) and El Niño (MEI)] and regional-scale wind-driven upwelling. We found no significant increase/decrease in MHW/MCS frequency, duration, or intensity over the last four decades, but did observe considerable interannual variability linked with basin-scale climate modes. Additionally, there was a decrease in both MHW/MCS occurrence during the upwelling season, and the initiation of individual MHWs/MCSs coincided with anomalous upwelling. Most notably, the co-occurrence of warm (cold) phases of the PDO and MEI with negative (positive) upwelling anomalies strongly enhanced the relative frequency of positive (negative) temperature anomalies and MHW (MCS) days. Collectively, both basin-scale variability and upwelling forcing play a key role in predicting extreme events and shaping nearshore resilience in EBUS.
Comparative biological activity of abamectin formulations on root-knot nematodes (Meloidogyne spp.) infecting cucumber plants: in vivo and in vitro
Authors: Massoud Magdy A. , Saad Abdel Fattah S. A. , Khalil Mohamed S. , Zakaria Mosher , Selim Shady 
Published: July 2023

AbstractThe root-knot nematodes (Meloidogyne spp.) are considered one of the most destructive diseases in the world. In Egypt, farmers primarily rely on chemical nematicides, which have become costly to control. Currently, abamectin is a bio-based pesticide used as an alternative tool against Meloidogyne spp. on cucumber plants (Cucumis sativus L.). During the current research, four tested abamectin formulations were DIVA (1.8% EW), RIOMECTIN (5% ME), AGRIMEC GOLD (8.4% SC) and ZORO (3.6% EC) compared with two reference nematicides namely, CROP NEMA (5% CS) and TERVIGO (2% SC). The main results showed that, in vitro study elucidated that the most effective formulations of abamectin as a larvicidal were EW with LC50 value of 21.66 µg ml−1. However, in the egg hatching test, the formulations of abamectin SC (2%) and EW were the most effective in reducing egg hatching, with LC50 values of 12.83 and 13.57 µg ml−1. The calculated relative potency values showed diversity depending on the two referenced nematicides. On the other hand, in vivo study, the results indicated that, all tested formulations of abamectin recorded general mean reductions in root galls (23.05–75.23%), egg masses (14.46–65.63%). Moreover, the total population density declined by 39.24–87.08%. Furthermore, the influence of abamectin formulations, in the presence of root-knot nematodes, on the growth of cucumber plants parameters, such as root dry weight, root length, root radius, root surface area, shoot dry weight and shoot height, as well as the content of macro-elements (N, P and K) exhibited varying levels of response.
Seroprevalence of leptospirosis among blood donors in an endemic area
Authors: Limothai Umaporn , Tachaboon Sasipha , Dinhuzen Janejira , Singh Jasleen , Jirawannaporn Sirawit , Leewongworasingh Akarathep , Thongpin Matsaya , Brameld Suppalak , Watanaboonyongcharoen Phandee , Sitprija Visith , Tantawichien Terapong , Thisyakorn Usa , Srisawat Nattachai 
Published: July 2023

AbstractThailand is known to be endemic for leptospirosis. This bacterium may pose a potential risk to transfusion safety. This study was a cross-sectional study examining the seroprevalence of leptospirosis among Thai blood donors. A total of 1053 serum specimens collected from blood donors residing in 5 regions of Thailand during March to September 2020 were included in this study. All samples were tested for the presence of antibodies to 22 leptospiral serovars using the microscopic agglutination test (MAT) and anti-Leptospira IgG antibodies using commercially available enzyme immunoassay. We found no evidence of recent exposure to Leptospira spp. in sera of healthy Thai blood donors by MAT, including those in higher-risk areas. However, in this same group, we did find small numbers of past exposure (1.7%) to Leptospira spp. by IgG ELISA. According to the findings of this study, there is currently no evidence for implementing new blood banking procedures to identify possible carriers in Thailand, however these should be continually monitored and revised according to the infectious disease burden in each country. It should be noted that there was a difference in the occupation rate between the general population reported in Thailand and blood donors in this study; it may not reflect the actual situation in the country.
Research on the anti-ageing mechanism of Prunella vulgaris L.
Authors: Li Ping , Lv Xiao , Wang Junrong , Zhang Chenyang , Zhao Jiahao , Yang Yadong 
Published: July 2023

AbstractPrunella vulgaris L. (P. vulgaris) has long been considered to have antipyretic, analgesic and anti-inflammatory effects, lowering blood lipids and pressure. Many studies show that in addition to the traditional telomere attrition, DNA damage and epigenetic changes, immunosenescence is also a new possibility to explore the mechanism of ageing. Therefore, this herb may have potential anti-ageing effects. Typically, there are a series of markers that identify senescent cells, such as superoxide dismutase (SOD)2, an inhibitor of CDK4 (p16INK4A), tumor necrosis factor (TNF)-α, immune cells number, proliferation, and nuclear abnormalities. These changes rarely present in young tissues, while greatly increasing in response to ageing. Firstly, the ageing model of the Institute of Cancer Research (ICR) mouse was established by d-galactose subcutaneous injection. Then, SOD2, p16INK4A and TNF-α were detected by quantitative Real-time PCR (qPCR), Western Blot (WB) and Enzyme-Linked Immunosorbent Assay (ELISA). Simultaneously, senescent cells in livers were stained by hematoxylin and eosin (HE). The viability of splenocytes was detected by Cell Counting Kit-8(CCK-8). The difference in specific immune cells (NK cells, B lymphocytes and T lymphocytes) was detected by flow cytometry. Both low (100 mg/kg) and high (300 mg/kg) concentrations of P. vulgaris treated ageing ICR mice show anti-ageing alterations, such as p16INK4A decreased approximately 1/2 and SOD2 tripled in livers, TNF-α decreased from 1 to 0.6 in plasma, and T cells increased from 0.09 to 0.19%. Compared with the ageing group, the spleen cells in the Prunella-treated group had stronger proliferation ability. Thus, P. vulgaris could have an anti-ageing effect. This is the first study to demonstrate the anti-ageing effect of P. vulgaris. It may also be capable of preventing a variety of age-related diseases.
Structural insights into selective small molecule activation of PKG1α
Authors: Metwally Essam , Mak Victor , Soriano Aileen , Zebisch Matthias , Silvestre H. Leonardo , McEwan Paul A. , Ermakov Grigori , Beaumont Maribel , Tawa Paul , Barker John J. , Yen Rose , Patel Akash , Lim Yeon-Hee , Healy David , Hanisak Jennifer , Cheng Alan C. , Greshock Tom , Fischmann Thierry O. 
Published: July 2023

AbstractcGMP-dependent protein kinase I-α (PKG1α) is a target for pulmonary arterial hypertension due to its role in the regulation of smooth muscle function. While most work has focused on regulation of cGMP turnover, we recently described several small molecule tool compounds which were capable of activating PKG1α via a cGMP independent pathway. Selected molecules were crystallized in the presence of PKG1α and were found to bind to an allosteric site proximal to the low-affinity nucleotide binding domain. These molecules act to displace the switch helix and cause activation of PKG1α representing a new mechanism for the activation and control of this critical therapeutic path. The described structures are vital to understanding the function and control of this key regulatory pathway.
Chromosome-level genome assembly and population genomics of Robinia pseudoacacia reveal the genetic basis for its wide cultivation
Authors: Wang Zefu , Zhang Xiao , Lei Weixiao , Zhu Hui , Wu Shengdan , Liu Bingbing , Ru Dafu 
Published: July 2023

AbstractUrban greening provides important ecosystem services and ideal places for urban recreation and is a serious consideration for municipal decision-makers. Among the tree species cultivated in urban green spaces, Robinia pseudoacacia stands out due to its attractive flowers, fragrances, high trunks, wide adaptability, and essential ecosystem services. However, the genomic basis and consequences of its wide-planting in urban green spaces remains unknown. Here, we report the chromosome-level genome assembly of R. pseudoacacia, revealing a genome size of 682.4 Mb and 33,187 protein-coding genes. More than 99.3% of the assembly is anchored to 11 chromosomes with an N50 of 59.9 Mb. Comparative genomic analyses among 17 species reveal that gene families related to traits favoured by urbanites, such as wood formation, biosynthesis, and drought tolerance, are notably expanded in R. pseudoacacia. Our population genomic analyses further recover 11 genes that are under recent selection. Ultimately, these genes play important roles in the biological processes related to flower development, water retention, and immunization. Altogether, our results reveal the evolutionary forces that shape R. pseudoacacia cultivated for urban greening. These findings also present a valuable foundation for the future development of agronomic traits and molecular breeding strategies for R. pseudoacacia.
A genome-wide association study identifies 41 loci associated with eicosanoid levels
Authors: Rhee Eugene P. , Surapaneni Aditya L. , Schlosser Pascal , Alotaibi Mona , Yang Yueh-ning , Coresh Josef , Jain Mohit , Cheng Susan , Yu Bing , Grams Morgan E. 
Published: July 2023

AbstractEicosanoids are biologically active derivatives of polyunsaturated fatty acids with broad relevance to health and disease. We report a genome-wide association study in 8406 participants of the Atherosclerosis Risk in Communities Study, identifying 41 loci associated with 92 eicosanoids and related metabolites. These findings highlight loci required for eicosanoid biosynthesis, including FADS1-3, ELOVL2, and numerous CYP450 loci. In addition, significant associations implicate a range of non-oxidative lipid metabolic processes in eicosanoid regulation, including at PKD2L1/SCD and several loci involved in fatty acyl-CoA metabolism. Further, our findings highlight select clearance mechanisms, for example, through the hepatic transporter encoded by SLCO1B1. Finally, we identify eicosanoids associated with aspirin and non-steroidal anti-inflammatory drug use and demonstrate the substantial impact of genetic variants even for medication-associated eicosanoids. These findings shed light on both known and unknown aspects of eicosanoid metabolism and motivate interest in several gene-eicosanoid associations as potential functional participants in human disease.