bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the impact of continuous nursing and cluster nursing on carpal tunnel syndrome release surgery
Authors: Chen Yang , Li Ruihua , Zhu Yongqiang , Chen Ran 
Published: January 2025

Abstract
Background
This study aimed to estimate the influence of continuous and cluster nursing on carpal tunnel syndrome (CTS) release surgery.

Methods
Ninety-six patients with CTS were treated in our hospital from November 2019 to December 2021. These patients were randomly divided into two groups of 48 patients. Both groups underwent open carpal tunnel release surgery. The control group received routine nursing care, while the study group received a combination of continuous and cluster nursing interventions. The Boston carpal tunnel question, the Visual Analogue Scale (VAS), the Numerical Rating Scale (NRS), the Pittsburgh Sleep Quality Index (PSQI), Disabilities of the Arm, Shoulder, and Hand questionnaire (DASH) and the Barthel index were used to compare hand function recovery, hand pain, sleep quality, and quality of life between the two groups before and 3 months after surgery.

Results
Both groups experienced improvements in hand function and pain 3 months after surgery. However, the study group demonstrated lower scores in symptom and dysfunction, as well as lower VAS and NRS scores compared to the control group (P < 0.05). Additionally, both groups showed an increase in the Barthel and PSQI scores 3 months after surgery. Notably, the study group exhibited higher Barthel scores and lower PSQI scores than the control group (P < 0.05).

Conclusion
The use of continuous and cluster nursing in patients undergoing CTS release surgery proves to be advantageous in alleviating hand pain, facilitating hand function recovery, and effectively enhancing sleep quality and overall quality of life for patients.
The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats
Authors: Shalaby Omnia E. , Ahmed Yasmine H. , Mekkawy Aya M. , Mahmoud Mohamed Y. , Elbargeesy G. A. 
Published: January 2025

Abstract
Background
Recently, silver nanoparticles (Ag-NPs) were shown to provoke oxidative stress through the release of reactive oxygen species and consequently induce cell damage. Selenium-loaded chitosan nanoparticles (CS-SeNPs) have anti-inflammatory and antioxidant effects, indicating that they ameliorate Ag-NPs-induced ovarian toxicity.

Objective
This study aimed to assess how well CS-SeNPs counteract the damaging effects of Ag-NPs on the ovarian tissue of adult female albino rats.

Methods
Forty mature female albino rats were divided into four equal groups: for 60 days, Group I (control) was given 0.5 ml/kg of distilled water; Group II was given Ag-NPs orally (100 mg/kg); Group III was given Ag-NPs orally (100 mg/kg/d) plus CS-SeNPs (0.5 mg/kg/d); and Group IV was given only CS-SeNPs orally (0.5 mg/kg/d). All the ovarian tissues were removed and underwent immunohistochemical, histological, and biochemical analyses.

Results
Ag-NPs-exposed rats revealed a marked reduction in reduced glutathione (GSH) and superoxide dismutase (SOD). Numerous histopathological alterations were found along with a significant increase in PCNA- and Caspase-3-immunoreactive cells. Most of these alterations were successfully ameliorated by CS-SeNPs, as indicated by marked increases in GSH and SOD.

Conclusion
CS-SeNPs ameliorate the toxic effects of Ag-NPs on the ovarian tissue of adult female albino rats.
DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage
Authors: Yang Qiao , Feng Yaya , Liu Lizhen , Yang Linlin , Wang Mingshu , Wu Ying , Tian Bin , Ou Xumin , Jia Renyong , Zhu Dekang , Chen Shun , Liu Mafeng , Zhao Xinxin , Zhang Shaqiu , Huang Juan , Sun Di , He Yu , Wu Zhen , Zhang Ling , Yu Yanling , Cheng Anchun 
Published: January 2025

AbstractIn herpesvirus, the terminase subunit pUL15 is involved in cleavage of the viral genome concatemers in the nucleus. Previous studies have shown that herpes simplex virus 1 (HSV-1) pUL15 can enter the nucleus without other viral proteins and help other terminase subunits enter the nucleus. However, this study revealed that duck plague virus (DPV) pUL15 cannot localize independently to the nucleus and can only be localized in the nucleus in the presence of pUL28 and pUL33. However, the data suggested the presence of a potential nuclear localization signal (NLS) in DPV pUL15, which is important for the localization of the terminase subunits. Subsequently, several single-point mutants were constructed to identify the vital amino acids within the NLS. The conserved amino acids K187, R188, and K190 are critical for the nuclear localization of pUL15, pUL28, and pUL33 but not for their interaction. Furthermore, corresponding recombinant viruses were constructed. The results revealed that the mutations rUL15K187Q, rUL15K188Q and rUL15K190Q had an obvious influence on concatemeric genome cleavage, but only K190Q significantly affected the production of progeny virions. These findings indicate that the NLS is important for the functions of DPV pUL15. Overall, a potential NLS and the key amino acids in DPV pUL15 were identified. Mutations in K187, K188 and K190 affected the cleavage of the concatemeric genome, but only mutations in K190 had an obvious effect on viral proliferation.
Analyzing environmental delivery of industrial technology and energy use-loss in China
Authors: Naimoğlu Mustafa , Alola Andrew Adewale 
Published: January 2025

AbstractThis study examines key indicators impacting China’s industrial sector: carbon dioxide (CO2) emissions by industry, industrial value added per worker, technology advancement in industrial design, fossil fuel usage, and energy losses in production and transmission. China’s industrial sector, heavily reliant on fossil fuels, has significant implications for environmental quality, establishing the country as the leading global carbon emitter. Rising energy losses compound economic challenges by driving up energy demand, increasing external dependencies, and straining foreign exchange reserves. In this context, we analyze how China’s industrial environment responds to technology adoption, energy losses, and fossil fuel usage. Using Fourier extensions enriched with time-series analyses, we assess the direction and magnitude of these responses, alongside testing the Environmental Kuznets Curve (EKC) hypothesis using Fully Modified Ordinary Least Squares, Canonical Cointegrating Regressions, and Dynamic Ordinary Least Squares methods. Our findings affirm the EKC hypothesis for China’s industrial sector, revealing that while fossil fuel usage and energy losses drive up CO2 emissions, advancements in industrial technology can offset some of these environmental impacts by reducing carbon emissions. Based on these findings, policymakers should focus on technological innovations that help decrease energy losses and fossil fuel consumption within the industrial sector.
Efficiency and heat transport processes of low-temperature aquifer thermal energy storage systems: new insights from global sensitivity analyses
Authors: Tas Luka , Hartog Niels , Bloemendal Martin , Simpson David , Robert Tanguy , Thibaut Robin , Zhang Le , Hermans Thomas 
Published: January 2025

AbstractAquifer thermal energy storage (ATES) has great potential to mitigate CO2 emissions associated with the heating and cooling of buildings and offers wide applicability. Thick productive aquifer layers have been targeted first, as these are the most promising hydrogeological context for ATES. Regardless, there is currently an increasing trend to target more complex aquifers such as low-transmissivity and alluvial aquifers or fractured rock formations. There, the uncertainty of subsurface characteristics and, with that, the risk of poorly performing systems is considerably higher. Commonly applied strategies to decide upon the ATES feasibility and well design standards for optimization need to be adapted. To further promote the use of ATES in such less favorable aquifers an efficient and systematic methodology evaluating the optimal conditions, while not neglecting uncertainty, is crucial. In this context, the distance-based global sensitivity analysis (DGSA) method is proposed. The analysis focuses on one promising thick productive aquifer, first used to validate the methodology, as well as a complex shallow alluvial aquifer. Through this method, multiple random model realizations are generated by sampling each parameter from a predetermined range of uncertainty. The DGSA methodology validates that the hydraulic conductivity, the natural hydraulic gradient and the annual storage volume dominate the functioning of an ATES system in both hydrogeological settings. The method also advances the state of the art in both settings. It efficiently identifies most informative field data ahead of carrying out the field work itself. In the studied settings, Darcy flux measurements can provide a first estimate of the relative ATES efficiency. It further offers a substantiated basis to streamline models in the future. Insensitive parameters can be fixed to average values without compromising on prediction accuracy. It also demonstrates the insignificance of seasonal soil temperature fluctuations on storage in unconfined shallow aquifers and it clarifies the thermal energy exchange dynamics directly above the storage volume. Finally, it creates the opportunity to explore different storage conditions in a particular setting, allowing to propose cutoff criteria for the investment in ATES. The nuanced understanding gained with this study offers practical guidance for enhanced efficiency of feasibility studies. It proves that the DGSA methodology can significantly speed up the development of ATES in more complex hydrogeological settings.
Hypertrophic cardiomyopathy: insights into pathophysiology and novel therapeutic strategies from clinical studies
Authors: Olalekan Samuel Oluwadare , Bakare Olalekan Olanrewaju , Okwute Patrick Godwin , Osonuga Ifabunmi Oduyemi , Adeyanju Muinat Moronke , Edema Victoria Biola 
Published: January 2025

Abstract
Background
Hypertrophic cardiomyopathy (HCM) is a frequently encountered cardiac condition worldwide, often inherited, and characterized by intricate phenotypic and genetic manifestations. The natural progression of HCM is diverse, largely due to mutations in the contractile and relaxation proteins of the heart. These mutations disrupt the normal structure and functioning of the heart muscle, particularly affecting genes that encode proteins involved in the contraction and relaxation of cardiac muscle.

Main body
This review focused on understanding the role of contractile and relaxation proteins in the pathogenesis of hypertrophic cardiomyopathy. Mutations in contractile proteins such as myosin, actin, tropomyosin, and troponin are associated with hypercontractility and increased sensitivity of the heart muscle, leading to HCM. Additionally, impaired relaxation of the heart muscle, linked to abnormalities in proteins like phospholamban, sarcolipin, titin, myosin binding protein-C, and calsequestrin, contributes significantly to the disease. The review also explored the impact of targeted therapeutic approaches aimed at modulating these proteins to improve patient outcomes. Recent advances in therapeutic strategies, including novel pharmacological agents like mavacamten and aficamten, were examined for their potential to help patients manage the disease and lead more accommodating lifestyles.

Conclusions
The review underscored the significance of early diagnosis and personalized treatment approaches in managing HCM. Future research should prioritize the development of robust biomarkers for early detection and risk stratification, particularly in diverse populations, to enhance clinical outcomes. Furthermore, it is imperative to delve deeper into the genetic mutations and molecular mechanisms associated with HCM, with a focus on exploring the roles of less-studied myocardial relaxation proteins and their interactions with sarcomere constituents.
EBUS-TBNA in diagnosis of pulmonary hamartoma: is it possible?
Authors: Emam Wael , Hafez Fatma , Osman Nehad 
Published: January 2025

Abstract
Background
Pulmonary hamartoma is an extremely rare benign neoplasm of the lungs. Patients usually present in the 4th and 5th decades of life and usually present as a hilar mass that needs to be biopsied to confirm the nature of the lesion. We report a case of pulmonary hamartoma that was diagnosed with EBUS-TBNA.

Case presentation
A 45-year-old female patient, non-smoker presented with dyspnea at moderate exertion. A CT scan showed a well-circumscribed left hilar mass. EBUS was planned and 3 passes EBUS-TBNA were taken and the final histopathological diagnosis was surprisingly pulmonary hamaroma.

Conclusions
EBUS-TBNA allows to locating of undiagnosed mediastinal lesions and performing a targeted needle aspiration, in a less invasive way, that can provide enough material for a certainty diagnosis in mediastinal hamartoma disease.
Concomitant Statin Use and Survival in Patients With Cancer on Immune Checkpoint Inhibitors: A Meta-Analysis
Authors: Liao Yonghe , Lin Yuxuan , Ye Xinqi , Shen Jinhai 
Published: January 2025

PURPOSE
The prognostic significance of concomitant statin use in cancer treatment with immune checkpoint inhibitors (ICIs) remains a subject of ongoing investigation. This study aims to clarify the prognostic value of statin use in this patient population and to provide a robust, evidence-based foundation to guide therapeutic decisions.

METHODS
A systematic search strategy was used across a multitude of digital archives to exhaustively identify all relevant academic literature published up until June 20, 2024. Studies published in English that reported hazard ratios (HRs) for overall survival (OS) and/or progression-free survival (PFS), along with corresponding 95% CIs, were considered eligible for inclusion. Meta-analyses were conducted to calculate combined HRs with 95% CIs.

RESULTS
A total of 25 studies, involving 46,154 patients with cancer, were included in the meta-analysis. The pooled results indicated that concomitant statin use was linked to better OS (HR, 0.80 [95% CI, 0.71 to 0.92]) and PFS (HR, 0.80 [95% CI, 0.69 to 0.92]) in patients with cancer under ICI therapy. Sensitivity analyses further validated the consistency and robustness of the combined results.

CONCLUSION
On the basis of the available clinical evidence, the concomitant use of statin is linked to an improved prognosis in oncology patients on ICI-based therapy. These observations underscore the potential of statin as an important adjunctive therapy in the treatment paradigm for ICI-treated patients with cancer, thereby establishing their significance as a key consideration in clinical management strategies. Further randomized controlled trials are imperative to validate the effect of statin use within the realm of ICI therapy.
Optimizing Radiotherapy Timing for Nasopharyngeal Carcinoma: The Impact of Radiation Scheduling on Survival
Authors: Li Ying , Weng Youliang , Huang Zongwei , Wu Lishui , Xu Siqi , Xie Yingjie , Li Haolan , Lai Jinghua , Hu Dan , Qiu Sufang 
Published: January 2025

PURPOSE
Chronoradiobiology has emerged as a potential field of study with therapeutic implications for cancer treatment. We aimed to investigate the association between radiation chronotherapy and the efficacy and toxicity of patients with nasopharyngeal carcinoma (NPC).

PATIENTS AND METHODS
Patients with nonmetastatic NPC treated with intensity-modulated radiotherapy in Fujian Cancer Hospital between January 2017 and December 2019 were included. Propensity score matching (PSM) with 1:1:1 was used to account for selection bias. Cox regression analysis was performed to explore the impact of radiotherapy timing on patient survival. Sensitivity analysis was implemented to determine the size and directional stability.

RESULTS

One thousand forty patients met study inclusion criteria and 332 patients were included in a PSM cohort. In the unmatched cohort analysis, morning radiotherapy exhibited a significantly superior overall survival (OS) outcome (hazard ratio [HR], 0.60 [95% CI, 0.40 to 0.91], adjusted log-rank
P
= .028) than the afternoon one. After PSM analysis, it was observed that individuals undergoing radiotherapy in the afternoon group (HR, 5.88 [95% CI, 2.55 to 13.58], adjusted log-rank
P
= .004) and the night group (HR, 4.81 [95% CI, 1.91 to 12.11], adjusted log-rank
P
= .018) displayed a tendency toward shorter OS compared with the morning group. No significant differences in acute treatment-related adverse effects were observed among the three groups. Morning radiotherapy demonstrated consistent robustness in the multivariable analysis, thereby establishing an association with higher OS. The directionality of the effect size was consistent across sensitivity analysis.

CONCLUSION
These results underscore the potential benefits of scheduling radiotherapy in the morning for NPC management, although prospective studies are needed to confirm these findings.
Digenic Inheritance Mode in Congenital Hypothyroidism Due to Thyroid Dysgenesis: HYPOTYGEN Translational Cohort Study
Authors: Stoupa Athanasia , Kariyawasam Dulanjalee , Jabot-Hanin Fabienne , Nguyen-Quoc Adrien , Hanein Sylvain , Rabeony Tioka , Elie Caroline , Colas Sandra , Thalassinos Caroline , Oliver-Petit Isabelle , Houang Muriel , Coutant Régis , Barat Pascal , Nicolino Marc , Reynaud Rachel , de Kerdanet Marc , Bensignor Candace , Baron Sabine , Raynaud-Ravni Catherine , Souchon Pierre-François , Léger Juliane , Castanet Mireille , Bole-Feysot Christine , Nitschke Patrick , Lyonnet Stanislas , Polak Michel , Carré Aurore 
Published: January 2025

Abstract

Context
Congenital hypothyroidism (CH) is the most common neonatal endocrine disorder and is chiefly caused by thyroid dysgenesis (CHTD). The inheritance mode of the disease remains complex.

Objective
Gain insight into the inheritance mode of CHTD.

Methods
Prospective multicenter nationwide translational study in France including 514 patients with CH diagnosed through systematic newborn screening (HYPOTYGEN cohort). We focused on CHTD cases and studied their clinical and molecular phenotypes. Targeted next-generation sequencing using a 78-gene panel, including genes involved in thyroid development, function, transport, metabolism and action of thyroid hormones. Statistical analysis, familial segregation, and in vitro functional studies focusing on cell migration have been performed.

Results
We analyzed the clinical phenotypes of 458 patients with CH. Cardiac and renal malformations were present in 7.7% (14/182) and 3.9% (7/178) of patients, respectively. Genetic analysis was performed on 292 patients of the cohort, based on criteria for ethnicity and availability of DNA samples for index cases and their parents. A disease-causing mutation in 1 of the 10 known genes for CHTD was identified in 20/292 (6.8%) patients. We found a digenic mode of inheritance in 16 (5.5%) patients, each carrying a variant in a thyroid development gene and a variant in the H2O2 generation complex gene DUOX2/DUOXA2. Familial segregation analysis and in vitro functional studies supported this model.

Conclusion
This work expands our understanding of the molecular causes of CHTD by demonstrating that digenic inheritance can be implicated, with deleterious variants in thyroid development and DUOX2/DUOXA2 genes. The complexity of this model implies a revision of the genetic landscape of CHTD and specific clinical care of patients during long-term follow-up.
Optimized husbandry and targeted gene-editing for the cnidarian Nematostella vectensis
Authors: Carvalho João E. , Burtin Maxence , Detournay Olivier , Amiel Aldine R. , Röttinger Eric 
Published: January 2025

ABSTRACT
Optimized laboratory conditions for research models are crucial for the success of scientific projects. This includes controlling the entire life cycle, having access to all developmental stages and maintaining stable physiological conditions. Reducing the life cycle of a research model can also enhance the access to biological material and speed up genetic tool development. Thus, we optimized the rearing conditions for the sea anemone Nematostella vectensis, a cnidarian research model, to study embryonic and post-metamorphic processes, such as regeneration. We adopted a semi-automated aquaculture system for N. vectensis and developed a dietary protocol optimized for the different life stages. Thereby, we increased spawning efficiencies, juvenile growth and survival rates, and considerably reduced the overall life cycle down to 2 months. To further improve the obtention of CRISPR-Cas9 mutants, we optimized the design of sgRNAs leading to full knockout animals in F0 polyps using a single sgRNA. Finally, we show that NHEJ-mediated transgene insertion is possible in N. vectensis. In summary, our study provides additional resources for the scientific community that uses or plans to use N. vectensis as a research model.
Transcriptional dynamics during karyogamy in rice zygotes
Authors: Toda Erika , Koshimizu Shizuka , Kinoshita Atsuko , Higashiyama Tetsuya , Izawa Takeshi , Yano Kentaro , Okamoto Takashi 
Published: January 2025

ABSTRACT
Upon fertilization, male and female nuclei fuse to form the zygotic nucleus in angiosperms. Karyogamy is considered to be essential for proper embryogenesis; however, the transcriptional dynamics during karyogamy in plant zygotes remain unclear. In this study, we performed a single-cell transcriptome analysis of rice zygotes at six early developmental stages (15 min, 30 min, 1 h, 2 h, 4 h, and 6 h after gamete fusion) to reveal gene expression profiles during karyogamy in plant zygotes. The time-series RNA-sequencing analysis detected possible de novo and altered gene expression in zygotes from 15 min post-fertilization. Fertilization-induced transcription during karyogamy was characterized by protein interaction database and gene ontology (GO) analyses. Furthermore, paternal allele transcription was initiated approximately 30 min to 1 h after gamete fusion, when nuclear fusion begins in the zygote. Some transcripts preferentially expressed in egg cells were downregulated after gamete fusion. Moreover, a dynamic shift from maternal-biased transcripts to bi-parental expression occurred during early zygotic development. These results suggest that transcriptional dynamics during karyogamy plays an initial role in proper and sequential zygotic development and embryogenesis.