bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blowing in the wind: Experimental assessment of clinging performance and behaviour in Anolis lizards during hurricane‐force winds
Authors: DiPaolo Emma C. C. , Kolbe Jason J. 
Published: January 2023

Abstract

Extreme weather events, such as hurricanes, can be ecologically devastating and cause widespread mortality. Recent studies in Anolis lizards report hurricane‐induced phenotypic shifts and selection favouring morphological variation related to clinging performance. Although it is difficult to observe organismal responses during extreme events in nature, we can experimentally simulate the high‐speed winds associated with hurricanes to evaluate the putative mechanism underlying observed patterns of natural selection.

In this study, we used two laboratory experiments to better understand the clinging performance and behaviour of Anolis lizards when experiencing hurricane‐force winds. We assessed the physical ability of lizards when using the combined function of their claws, limbs, toepads and other traits to resist forces pulling them off a perch. We also evaluated the combination of this physical clinging ability of lizards and their behavioural responses to avoid being blown off a perch during high winds. We assessed behaviour that could decrease exposure of lizards to wind and increase their clinging ability.

Clinging force measurements revealed variation in performance among species and substrates not reflected in clinging times for lizards experiencing hurricane‐force winds, revealing the importance of behaviour when experiencing high winds. The most arboreal species (A. carolinensis) had substantially longer clinging times on rough substrates compared with the other species, presumably due to its larger toepads for increased clinging as well as its shorter limbs that reduced drag.

Under high‐speed winds, lizards commonly shifted to the more protected leeward side of dowels, especially on broad and rough substrates, presumably to reduce exposure. This reveals how behaviour can mediate factors influencing clinging ability during hurricanes and, in conjunction with ecologically relevant variation in morphology and substrate properties, contribute to clinging performance.

Our experiments reveal that behaviour strongly influences clinging performance during high winds beyond that predicted by physical traits alone. Thus, microhabitat selection of perches and the position of a lizard on its perch during a hurricane will likely have important consequences for clinging performance. This may alter how selection acts on morphological traits and influence the susceptibility of different species to these extreme weather events.

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Synthetic Nasonov gland pheromone enhances abundance and visitation of honeybee, Apis mellifera, in Korla fragrant pear, Pyrus sinkiangensis
Authors: Li Qian , Sun Mengxiao , Liu Yangtian , Liu Bing , van der Werf Wopke , Bianchi Felix J. J. A. , Lu Yanhui 
Published: January 2023

Abstract
Korla fragrant pear (Pyrus sinkiangensis Yü) depends on cross‐pollination by honeybees (Apis mellifera) but may suffer from low honeybee visitation.
We assessed whether honeybee abundance and visitation frequency are enhanced by using synthetic Nasonov gland pheromone (NGP), which is naturally produced by worker bees to stimulate the aggregation of bees to food resources or nesting sites.
The response of honeybees to synthetic NGP was firstly assessed using Y‐tube olfactometer tests in the laboratory, and subsequently in the field, by placing NGP lures on Korla fragrant pear trees in orchards with and without beehives. Honeybee abundance was assessed using coloured pan traps while honeybee visits were assessed by visual observations on pear flowers.
Y‐tube olfactometer tests showed a significant preference of honeybees for NGP. In pear orchards with beehives, honeybee abundance was 2.5‐fold higher on trees with NGP lures than on trees without NGP, and 2.2‐fold higher in orchards in which all trees contained NGP lures than in orchards without NGP lures. Such positive effects were not observed in orchards without beehives.
Flower visitation by honeybees was significantly higher in trees with NGP lures than without NGP lures, irrespective of the presence (5.7‐fold higher) or absence of beehives (27.6‐fold higher).
In mixed pear‐apricot orchards, honeybee abundance was higher in pear trees with NGP lures than without lures.
Our results show that NGP lures attract honeybees to flowering pear trees in monoculture pear and mixed pear‐apricot orchards, and that this effect is greatest in orchards with beehives.
Growth differentiation factor 15 is dispensable for acetaminophen‐induced liver injury in mice
Authors: Jiang Peng , Liu Zhenghong , Fang Tingyu , Zhang Zhidan , Zhang Yu , Wang Dongdong , Little Peter J. , Xu Suowen , Weng Jianping 
Published: January 2023

AbstractAcetaminophen (APAP)‐induced liver injury (AILI) has been recognized as a pivotal contributor to drug‐induced liver failure in Western countries, but its molecular mechanism remains poorly understood. Growth differentiation factor 15 (GDF15) is a pleiotropic factor that alleviates non‐alcoholic liver steatohepatitis, liver fibrosis and liver injury. The aim of the present study was to examine the possibility whether GDF15 confers protection against AILI. We found that the gene expression of Gdf15 was increased significantly after APAP overdose in mice. Next, the role of Gdf15 in AILI was evaluated by hepatic Gdf15 overexpression (using adeno‐associated virus serotype 8), injection with recombinant human GDF15 (rhGDF15) and Gdf15 knockout mice after challenge with APAP. A marked elevation of Gdf15 was observed after AILI. However, there were no significant differences in AILI‐related liver injury and JNK phosphorylation after Gdf15 overexpression, rhGDF15 injection or Gdf15 deficiency. Together, we conclude that, despite a noticeable elevation of Gdf15 level after AILI, Gdf15 is dispensable for APAP‐induced AILI. Our study further suggests that genomic analysis of mRNA expression after APAP overdose is of limited relevance unless followed up by a functional analysis of candidate genes in vivo.
Adjusting for Publication Bias in Meta-Analysis via Inverse Probability Weighting Using Clinical Trial Registries
Authors: Huang Ao , Morikawa Kosuke , Friede Tim , Hattori Satoshi 
Published: January 2023

Abstract
Publication bias is a major concern in conducting systematic reviews and meta-analyses. Various sensitivity analysis or bias-correction methods have been developed based on selection models, and they have some advantages over the widely used trim-and-fill bias-correction method. However, likelihood methods based on selection models may have difficulty in obtaining precise estimates and reasonable confidence intervals, or require a rather complicated sensitivity analysis process. Herein, we develop a simple publication bias adjustment method by utilizing the information on conducted but still unpublished trials from clinical trial registries. We introduce an estimating equation for parameter estimation in the selection function by regarding the publication bias issue as a missing data problem under the missing not at random assumption. With the estimated selection function, we introduce the inverse probability weighting (IPW) method to estimate the overall mean across studies. Furthermore, the IPW versions of heterogeneity measures such as the between-study variance and the I2 measure are proposed. We propose methods to construct confidence intervals based on asymptotic normal approximation as well as on parametric bootstrap. Through numerical experiments, we observed that the estimators successfully eliminated bias, and the confidence intervals had empirical coverage probabilities close to the nominal level. On the other hand, the confidence interval based on asymptotic normal approximation is much wider in some scenarios than the bootstrap confidence interval. Therefore, the latter is recommended for practical use.
Puerarin from Pueraria lobate attenuates ischemia‐induced cardiac injuries and inflammation in vitro and in vivo: The key role of miR‐130a‐5p/HMGB2 pathway
Authors: Fan Chenrong , Wang Qizeng , Chen Youjin , Ye Tingting , Fan Yuncao 
Published: January 2023

AbstractAcute myocardial infarction (AMI) is a common cardiovascular disease and puerarin (Pue) is an active compound from Pueraria lobate with cardio‐protective potential. In the current study, the mechanism underlying the cardio‐protective effects of Pue was explored by focusing miR‐130a‐5p/HMGB2 pathway. MiR expression profile was determined and myocardial infarction was induced in cardiomyocytes and rats, which was treated with Pue. The role of miR‐130a‐5p and downstream HMGB2/NF‐κB axis in the cardio‐protective effects of Pue was also explored. Pue increased viability and suppressed inflammation in OGD cardiomyocytes, which was associated with the deactivation of HMGB2/NF‐κB pathway. After the suppression of miR‐130a‐5p, the cardio‐protective effects of Pue were compromised. In rat models, Pue attenuated structure deterioration and inflammatory response in heart. At the molecular level, miR‐130a‐5p was up‐regulated, and HMGB2 were down‐regulated. It was demonstrated that Pue induced the expression of miR‐130a‐5p, which suppressed the activity of HMGB2/NF‐κB, contributing to the attenuation of infarct heart tissues.
The divergent adolescent and adult transitions of Latin American undocumented minors
Authors: Diaz‐Strong Daysi Ximena , Gonzales Roberto G. 
Published: January 2023

AbstractUndocumented immigrants arriving in the United States as minors navigate tremendous constraints as they transition into adolescence and adulthood. Exclusionary immigration laws profoundly shape and complicate the attainment of important milestones and the decisions undocumented minors make about their adult futures. A significant body of research, largely focused on children who arrive when they are 12 years old and younger, has examined the impact of legal exclusion on the coming‐of‐age experiences of undocumented minors. However, we know less about the experiences of teenage arrivals. In this article, we focus on young Latin American migrants and provide an overview of how legal exclusion contours undocumented minors' adolescent and adult transitions; we also call attention to the divergent experiences of youth who arrive during childhood and youth who arrive during adolescence. Throughout the article, we emphasize how the timing of immigration—the developmental stage when a young person migrates—creates meaningful differences in the barriers undocumented minors confront.
Factors affecting the outcomes of tirofiban after endovascular treatment in acute ischemic stroke: Experience from a single center
Authors: Wang Yuan , Ma Hongrui , Zhang Qihan , Jin Feiyang , Xu Yi , Ma Qingfeng , Ji Xunming 
Published: January 2023

AbstractAimsTo investigate the predicted factors influencing the outcomes in acute ischemic stroke (AIS) patients who received tirofiban after endovascular treatment (EVT) and the optimal administration of tirofiban.MethodsIn this retrospective study, AIS patients who received EVT followed by tirofiban between January 2017 and October 2021 were enrolled. The dose and duration of tirofiban were adjusted by trained clinicians according to the patient's clinical status. A reduction of at least four points on the National Institutes of Health Stroke Scale (NIHSS) after tirofiban compared with that before tirofiban was defined as an effective response. A modified ranking scale (mRS) of 0–2 was defined as a favorable outcome at a 90‐day follow‐up.ResultsA total of 260 consecutive patients were enrolled, and 36.5% of patients achieved a favorable outcome. The modified thrombolysis in cerebral infarction (mTICI) 2b‐3 occurred in 93.5% of patients. Symptomatic intracerebral hemorrhage (sICH) occurred in 6.2% of patients, and the mortality at 90‐day follow‐up was 16.9%. Duration of tirofiban >24 h (adjusted OR: 2.545; 95% CI: 1.008–6.423; p = 0.048) and effective response to tirofiban (adjusted OR: 25.562; 95% CI: 9.794–66.715; p < 0.001) were related to the favorable outcome (mRS 0–2). Higher NIHSS (adjusted OR: 0.855; 95% CI: 0.809–0.904; p < 0.001) and glucose level on admission (adjusted OR: 0.843; 95% CI: 0.731–0.971; p = 0.018) were predictive for the unfavorable outcome (mRS 3–6).ConclusionsAn effective response to tirofiban is an independent factor in predicting the long‐term efficacy outcome, and extending the duration of tirofiban is beneficial for neurological improvement.
Concentration‐dependent stimulation of melanin production as well as melanocyte and keratinocyte proliferation by melatonin in human eyelid epidermis
Authors: Sevilla Alec , Chéret Jérémy , Lee Wendy , Paus Ralf 
Published: January 2023

AbstractIt remains unclear how the multifunctional indoleamine neurohormone, melatonin, alters melanin production and melanocytes within intact human epidermis under physiologically relevant conditions. In the current pilot study, we aimed to clarify this in long‐term organ‐cultured, full‐thickness human eyelid skin, selected for its clinically recognized sensitivity to pigmentation‐modulatory hormones. Warthin‐Starry histochemistry showed that 100 μM melatonin significantly increased epidermal melanin content and melanocyte dendricity after 6 days of organ culture, even though tyrosinase activity in situ was inhibited, as assessed by quantitative immunohistomorphometry. While the higher melatonin dose tested here (200 μM) did not change epidermal melanization, but again inhibited tyrosinase activity, it increased the number and proliferation of both gp100+ epidermal melanocytes and keratinocytes as well as protein expression of the premelanosomal marker, gp100, ex vivo. Contrary to most previous studies, these eyelid skin organ culture results suggest that long‐term melatonin application exerts overall stimulatory, dose‐dependent effects on the epidermal pigmentary unit within intact human skin, which appear surprisingly tyrosinase‐independent. While these provocative preliminary findings require further work‐up and independent confirmation, they encourage one to systematically explore whether prolonged melatonin therapy can (re‐)stimulate melanogenesis and increase the pool/activity of epidermal melanocytes in hypopigmented skin lesions.
Effects of RuBisCO and CO2 concentration on cyanobacterial growth and carbon isotope fractionation
Authors: Garcia Amanda K. , Kędzior Mateusz , Taton Arnaud , Li Meng , Young Jodi N. , Kaçar Betül 
Published: January 2023

AbstractCarbon isotope biosignatures preserved in the Precambrian geologic record are primarily interpreted to reflect ancient cyanobacterial carbon fixation catalyzed by Form I RuBisCO enzymes. The average range of isotopic biosignatures generally follows that produced by extant cyanobacteria. However, this observation is difficult to reconcile with several environmental (e.g., temperature, pH, and CO2 concentrations), molecular, and physiological factors that likely would have differed during the Precambrian and can produce fractionation variability in contemporary organisms that meets or exceeds that observed in the geologic record. To test a specific range of genetic and environmental factors that may impact ancient carbon isotope biosignatures, we engineered a mutant strain of the model cyanobacterium Synechococcus elongatus PCC 7942 that overexpresses RuBisCO across varying atmospheric CO2 concentrations. We hypothesized that changes in RuBisCO expression would impact the net rates of intracellular CO2 fixation versus CO2 supply, and thus whole‐cell carbon isotope discrimination. In particular, we investigated the impacts of RuBisCO overexpression under changing CO2 concentrations on both carbon isotope biosignatures and cyanobacterial physiology, including cell growth and oxygen evolution rates. We found that an increased pool of active RuBisCO does not significantly affect the 13C/12C isotopic discrimination (εp) at all tested CO2 concentrations, yielding εp of ≈ 23‰ for both wild‐type and mutant strains at elevated CO2. We therefore suggest that expected variation in cyanobacterial RuBisCO expression patterns should not confound carbon isotope biosignature interpretation. A deeper understanding of environmental, evolutionary, and intracellular factors that impact cyanobacterial physiology and isotope discrimination is crucial for reconciling microbially driven carbon biosignatures with those preserved in the geologic record.
Satellite observed reversal in trends of spring phenology in the middle‐high latitudes of the Northern Hemisphere during the global warming hiatus
Authors: Xiong Tao , Du Shihong , Zhang Hongyan , Zhang Xiuyuan 
Published: January 2023

AbstractThe start of the growing season (SOS) is essential to track the responses of vegetation to climate change. However, recent findings on whether the SOS in the middle‐high latitudes of the Northern Hemisphere (NH) continued to advance or reversed during the global warming hiatus were not consistent. It is necessary to investigate the causes of this controversy and to examine the relationship between the SOS and preseason temperature trends. To this end, we first applied four widely used phenology extraction methods to derive the SOS from the GIMMS NDVI3g dataset and then used the ensemble empirical modal decomposition (EEMD) method to extract the nonlinear trends of the SOS and preseason temperature. Our results clarify, for the first time, that the limitations of the linear assumption‐based trend analysis methods are an important but overlooked cause of the discrepancies among existing studies on whether the SOS was advanced or delayed in the NH (>30° N) during the global warming hiatus. We further revealed the range of the mismatches between the SOS and preseason temperature trends at the latitude, altitude and biome levels. Specifically, we discovered that the SOS in the NH (>30° N) obtained by the four phenology extraction methods showed a significant reversal from advance to delay during the global warming hiatus, and the corresponding average rate of change was very small. The area showing increasing preseason temperatures decreased during the global warming hiatus, but it always occupied most of the NH (>30° N). However, delayed SOS trends were dominant in the NH from 50° N to 60° N, above 3000 m and in biomes other than TBMF and BF. Accordingly, using an EEMD‐like approach to evaluate the changes in the SOS and preseason temperature is necessary for improving our understanding of the changes in the SOS and their association with climate.
Constructing children's psychological well‐being: Sources of resilience for children left behind in Northeast China
Authors: Wang Shuai 
Published: January 2023

AbstractPopulation mobility has become an inevitable trend of social transformation in contemporary China. Parental migration has changed family structure patterns and brought great uncertainty to the psychological well‐being of left‐behind children. By comparing left‐behind children to children of non‐migrants, this study examines the impact of parental migration on children's psychological well‐being and the potential support of schools for children's resilience development along three dimensions: children's school belonging, teacher–student relationships and peer support. Using a mixed‐method approach, this study draws on an original survey of children (N = 605) and in‐depth interviews with children, parents, grandparents, and teachers (N = 41) in Hunchun City, Northeast China. Overall, maternal migration has a negative impact on children's psychological well‐being. In addition to teacher–student relationships, school belonging and peer support significantly mitigate this negative impact; qualitative data further suggest that peer support distracts from, rather than offsets, children's poor psychological well‐being associated with maternal migration.
Prevalence and predictive factors of depressive symptoms among primiparae in antenatal care: A cross‐sectional study in the new families programme
Authors: Øygarden Anne‐Martha Utne , Småstuen Milada C. , Abudayya Abdallah , Glavin Kari , Sæther Kristin Marie , Solberg Beate L. , Brekke Malene , Strøm Benedicte S. 
Published: January 2023

AbstractAim/ObjectiveTo estimate the prevalence and assess the strength of associations between antenatal depressive symptoms and selected possible predictive factors among women attending antenatal care for the first time at the Child Health Centre.BackgroundEvidence suggests that antenatal depression is a health problem as prevalent as postpartum depression. Antenatal depressive symptoms may persist into the postpartum period and potentially disturb the attachment between mother and family.DesignCross‐sectional.MethodsA sample of 228 women who participated in the New Families research programme answering a questionnaire at Week 28 of pregnancy were included. The Edinburgh Postnatal Depression Scale was used as outcome measure. Prevalence was estimated, and backward stepwise logistic regression analysis was performed to examine associations with somatic, psychiatric and social factors. STROBE checklist was followed.ResultsPrevalence of depressive symptoms in this sample was 17.9%. Women reporting not sleeping enough had significantly higher odds of having depressive symptoms. Pregnant women with high relationship satisfaction were less likely to have depressive symptoms. Neither maternal age, education, previous depression, coming from a non‐Nordic country nor having complications during pregnancy were statistically significantly associated with depressive symptoms.ConclusionsHigh prevalence of depressive symptoms may also be present in low‐risk populations. Sleep and relationship satisfaction are topics that need to be addressed during pregnancy.Relevance to Clinical PracticeThe prevalence of depressive symptoms in this sample of low‐risk pregnant women indicates that health personnel needs to be aware of depressive symptoms among women with no risk indicators. Identifying predictive factors associated with depressive symptoms may help midwives, public health nurses and general practitioners support parents. Our results indicate a potential need for screening for depressive symptoms in pregnancy and underscore the importance of involving partners in antenatal care.Patient/Public ContributionParents and health personnel participated in designing the New Family research programme.
The detailed obstetric course of the first Japanese patient with AEBP1‐related Ehlers–Danlos syndrome (classical‐like EDS, type 2)
Authors: Sanai Hiromi , Nakamura Yasuhiko , Koike Yuta , Murota Hiroyuki , Kosho Tomoki , Sase Masakatsu 
Published: January 2023

AbstractWe reported a detailed obstetric course of a Japanese patient with Ehlers–Danlos syndrome (EDS) caused by biallelic pathogenic variants in the AEBP1 gene. She was diagnosed with classical EDS at 3 years of age. At 33 years, whole‐exome sequencing revealed a homozygous nonsense variant (c.1894C > T:p.Arg632*) in AEBP1. This is the 10th case of AEBP1‐related EDS (classical‐like EDS type 2) and the first in Japan. She was managed as an inpatient at our hospital beginning at 20 weeks of gestation because of the possibility of high‐risk pregnancy. She experienced painful urinary retention, migraines, and threatened premature labor. She delivered a healthy female via elective caesarean section at 32 weeks of gestation. She was treated in the intensive care unit for severe paralytic ileus, postoperatively. Conservative therapy resulted in favorable outcomes, and she was safely discharged on postdelivery day 22nd.