bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribution, diversity and diversification from a DNA barcoding perspective: the case ofGammarusradiation in Europe’s oldest inland waterbody - the ancient Lake Ohrid
Authors: Mamos Tomasz , Grabowski Michał , Sworobowicz Lidia , Salzburger Walter , Trajanovski Sasho , Copilaş-Ciocianu Denis , Mucciolo Serena , Wysocka Anna 
Published: April 2023

AbstractAimA detailed, comparative DNA-barcoding and morphospecies based overview of the vertical and horizontal distribution of Lake Ohrid endemicGammarusspecies-flock. Re-evaluation of the origin of the species-flock dating, identification of events that putatively influenced diversification patterns in the species-flock.LocationLake Ohrid: a deep and ancient lake of tectonic origin, biosphere reserve, UNESCO World Heritage Site, located on the Macedonia/Albania border.TaxonGammarusspecies-flock (Amphipoda, Crustacea)MethodsExtensive sampling and DNA barcoding of 600 individuals were carried out. DNA sequences were analysed using species delimitation methods, haplotype network reconstructions, Bayesian molecular dating and demographic analysis. The COI-based delimitation results were validated with nuclear 28S RNA data.ResultsThe species flock distribution has weak horizontal but clear vertical structure. The diversity across bathymetric gradients correlates with temperature and salinity; and the highest diversity with sublittoral and springs of lake’s shore. Two new MOTUs representing putatively new species are revealed and supported also by the nuclear marker. The time of flock radiation overlaps with the time of lake formation. The COI gene shows signs of positive selection and an acceleration in substitution rate. The demographic changes of the flock happened during the last ky.Main conclusionsDistribution of theGammarusspecies-flock is vertically structured, reflecting habitat zonation. Parapatric speciation as one of the mechanisms in flock’s diversification is suggested. Detection of new MOTU suggests that the flock’s diversity is still not fully revealed. Nevertheless, failure to recover three other MOTUs suggests the loss of gammarid diversity in the lake. This represents,together with the current threats to the lake ecosystem (i.e. climate changes, development of tourism), a clear call for conservation efforts. The speciation events and demographic changes within the flock relate presumably to glacial and postglacial water level changes and to colonisation of new depth ranges and the associated springs.
Structural insight into an anti-BRIL Fab as a G-protein-coupled receptor crystallization chaperone
Authors: Miyagi Hikaru , Suzuki Michihiko , Yasunaga Mai , Asada Hidetsugu , Iwata So , Saito Jun-ichi 
Published: April 2023

Structure determination of G-protein-coupled receptors (GPCRs) is key for the successful development of efficient drugs targeting GPCRs. BRIL is a thermostabilized apocytochrome b
562 (with M7W/H102I/R106L mutations) from Escherichia coli and is often used as a GPCR fusion protein for expression and crystallization. SRP2070Fab, an anti-BRIL antibody Fab fragment, has been reported to facilitate and enhance the crystallization of BRIL-fused GPCRs as a crystallization chaperone. This study was conducted to characterize the high-resolution crystal structure of the BRIL–SRP2070Fab complex. The structure of the BRIL–SRP2070Fab complex was determined at 2.1 Å resolution. This high-resolution structure elucidates the binding interaction between BRIL and SRP2070Fab. When binding to BRIL, SRP2070Fab recognizes conformational epitopes, not linear epitopes, on the surface of BRIL helices III and IV, thereby binding perpendicularly to the helices, which indicates stable binding. Additionally, the packing contacts of the BRIL–SRP2070Fab co-crystal are largely due to SRP2070Fab rather than BRIL. The accumulation of SRP2070Fab molecules by stacking is remarkable and is consistent with the finding that stacking of SRP2070Fab is predominant in known crystal structures of BRIL-fused GPCRs complexed with SRP2070Fab. These findings clarified the mechanism of SRP2070Fab as a crystallization chaperone. Moreover, these data will be useful in the structure-based drug design of membrane-protein drug targets.
Can perceived coolness enhance memorable customer experience? The role of customer engagement
Authors: Tsaur Sheng-Hshiung , Teng Hsiu-Yu , Han Tien-Cheng , Tu Jin-Hua 
Published: April 2023

Purpose
Memorable customer experience (MCE) is a key factor affecting customer loyalty and revisit intention. Hospitality managers must identify factors that create MCEs. This study aims to investigate relationships among perceived coolness, customer engagement and MCE and examine the mediation effect of customer engagement.

Design/methodology/approach
Two samples of 434 restaurant customers and 372 hotel customers in Taiwan returned questionnaires. Hypotheses were examined by structural equation modeling.

Findings
The results demonstrated that perceived coolness positively affected customer engagement and MCE and that customer engagement positively affected MCE. Furthermore, customer engagement mediated the relationship between perceived coolness and MCE.

Research limitations/implications
This study is cross-sectional, which limits causal inference. Furthermore, this study only investigated customers of Taiwanese restaurants and hotels, and the findings may not be generalizable to other industries and countries.

Originality/value
This study contributes to the MCE knowledge in hospitality by elucidating the association among perceived coolness, customer engagement and MCE. The findings can aid hospitality managers in developing marketing strategies, fostering customer engagement and creating MCEs.
The coverage of information science and knowledge organization in theLibrary of Congress Subject Headings
Authors: Furner Jonathan , Hjørland Birger 
Published: April 2023

PurposeThis article examines theLibrary of Congress Subject Headings(LCSH), which is the most used subject heading system in the world and an instance of a controlled vocabulary (CV).Design/methodology/approachThe method used to examine the system is based on both authors’ subject knowledge in the field of information science (IS) and the subfield of knowledge organization (KO). Core concepts in this domain were examined (1) by checking if they are present or not in the system; (2) if not, by determining whether LCSH contains alternative terms useful for searching documents about the missing concept, by examining books indexed by the Library of Congress; (3) by identifying the semantic relations between subject headings.FindingsThe results demonstrate fundamental problems in the logical consistency of the representation of IS and KO in LCSH.Practical implicationsThe implications for CVs in general are discussed.Originality/valueNo previous study has used our method to examine LCSH’s coverage of IS.
Shaping the culture of your organization by the human capital: employees' competencies and leaders' perceived behavior
Authors: Popa Ştefan Cătălin , Ştefan Simona Cătălina , Olariu Ana Alexandra , Popa Cătălina-Florentina , Pantea Marius Ioan 
Published: April 2023

PurposeOrganizational culture (OC) is not only a key factor in the development of organizational performance but also an important source of employee orientation toward a better understanding of organizational goals. This study aims to analyze the influence of two individual factors on OC: (1) the competencies of employees and (2) perceived leadership behavior. The study also focuses on how the relationship between individual factors and OC differs between public and private organizations.Design/methodology/approachIn this study, data were collected through a survey, and a structural equation modeling approach, partial least squares structural equation modeling, was used to highlight the proposed direct, mediated and moderated relationships. The sample comprises 1,284 respondents, representing both public and private sector organizations.FindingsBased on the results, the employees' competencies positively and significantly influence the hierarchical and market orientation of OC. Additionally, the perceived leadership behavior positively and significantly influences the adoption of all four types of cultural characteristics (i.e. clan, adhocracy, market and hierarchy).Practical implicationsThe results are of great benefit to organizations who may become more aware that employees' skills and how employees perceive leadership behavior can significantly influence OC.Originality/valueThese findings make an important contribution to understanding how the characteristics of each type of OC can be influenced by certain behaviors, skills and perceptions and how those relationships may be shaped by the organization's ownership.
Changes in credit score, transaction volume, customer characteristics, and the probability of detecting suspicious transactions
Authors: Reite Endre J. , Oust Are , Bang Rebecca Margareta , Maurstad Stine 
Published: April 2023

Purpose
This study aims to use a unique customer-information data set from a Norwegian bank to identify how small changes in firm-specific factors correlate with the risk of a client subsequently being involved in suspicious transactions. It provides insight into the importance of updating client risk based on changes in transaction volume and credit risk to enable effective resource use in transaction monitoring.

Design/methodology/approach
Changes in a firm’s bank use and accounting data were tested against subsequent flagged and reported customers to identify which changes led to a significant increase in the probability of engaging in a transaction identified as suspicious. Prioritizing resources to firms that remain suspicious after further controls can improve the risk-based approach and prioritize detection efforts. The main factors were customer probability of default (credit score), size and changes in customer characteristics. The cross-sectional data set contained administrative data on 8,538 corporate customers (219 with suspicious transactions that were subsequently flagged, 64 of which were reported). A binomial logit model was used.

Findings
Changes in transaction volume and bank use are significant in predicting subsequent suspicious transactions. Customer credit score changes were significantly positively correlated with the likelihood of flagging and reporting. Change is a stronger indicator of suspicious transactions than the level. Thus, frequent updating of client risk and using a scale rather than risk categories can improve client risk monitoring. The results also showed that the current anti-money laundering (AML) system is size-dependent; the greater the change in customer size, the greater the probability of the firm subsequently engaging in a suspicious transaction.

Research limitations/implications
Client risk classification, monitoring changes in a client’s use of the bank and business risk should receive more attention.

Practical implications
The authors demonstrate that client risk classifications should be dynamic and sensitive to even small changes, including monitoring the client’s credit risk changes.

Social implications
Directing AML efforts to clients with characteristics indicating risk and monitoring changes in factors contributing to risk can increase efficiency in detecting money laundering.

Originality/value
To the best of the authors’ knowledge, this is the first study to focus on changes in a firm's use of a bank and link this to the probability of detecting a suspicious transaction.
Role of entrepreneurial resilience in SMEs to promote marketing and entrepreneurship amid Covid19 challenges
Authors: Pathak Mallika Devi , Kar Brajaballav , Panigrahi Rashmi Ranjan , Shrivastava Avinash K. 
Published: April 2023

Purpose
Resilient firms are more likely to survive crisis. This paper aims to investigate the resilient behavior displayed by small and medium enterprise (SME) owners in the pandemic context and the sequence of antecedents leading to resilient behavior.

Design/methodology/approach
Seven SME owners, from different business sectors, were interviewed twice in 8 months, to assess their actions and underlying entrepreneurial resilience. A model was developed depicting the sequence of activities leading to entrepreneurial resiliency behavior. The study was conducted in the capital city of Odisha, an eastern Indian state.

Findings
The results indicate that entrepreneurial resiliency is manifested in various forms where the SME owners engage in bricolage. Resiliency has an inevitable time dimension, where SME owners estimate the period for which adversity is likely to persist. They focus on alternative action to demonstrate resiliency. It was also learnt that revenue management during a crisis requires entrepreneurial marketing with innovativeness, opportunity seeking and value creation to improve resiliency.

Research limitations/implications
This research is important for policymakers who can strengthen resiliency through the support and provision of adequate information to SME owners. Educators can use the model for discussion and pedagogy. Finally, SME owners can evaluate their response behaviors to a crisis and draw insights.

Originality/value
The approach of the study was longitudinal and qualitative. This study contributes to the literature gap on resiliency in the context of emerging markets and SMEs.
Ecophysiological analysis reveals distinct environmental preferences in closely related Baltic Sea picocyanobacteria
Authors: Aguilera Anabella , Alegria Zufia Javier , Bas Conn Laura , Gurlit Leandra , Śliwińska‐Wilczewska Sylwia , Budzałek Gracjana , Lundin Daniel , Pinhassi Jarone , Legrand Catherine , Farnelid Hanna 
Published: April 2023

AbstractCluster 5 picocyanobacteria significantly contribute to primary productivity in aquatic ecosystems. Estuarine populations are highly diverse and consist of many co‐occurring strains, but their physiology remains largely understudied. In this study, we characterized 17 novel estuarine picocyanobacterial strains. Phylogenetic analysis of the 16S rRNA and pigment genes (cpcB and cpeBA) uncovered multiple estuarine and freshwater‐related clusters and pigment types. Assays with five representative strains (three phycocyanin rich and two phycoerythrin rich) under temperature (10–30°C), light (10–190 μmol photons m−2 s−1), and salinity (2–14 PSU) gradients revealed distinct growth optima and tolerance, indicating that genetic variability was accompanied by physiological diversity. Adaptability to environmental conditions was associated with differential pigment content and photosynthetic performance. Amplicon sequence variants at a coastal and an offshore station linked population dynamics with phylogenetic clusters, supporting that strains isolated in this study represent key ecotypes within the Baltic Sea picocyanobacterial community. The functional diversity found within strains with the same pigment type suggests that understanding estuarine picocyanobacterial ecology requires analysis beyond the phycocyanin and phycoerythrin divide. This new knowledge of the environmental preferences in estuarine picocyanobacteria is important for understanding and evaluating productivity in current and future ecosystems.
Pathogens identified in the internal tissues and placentas of stillbirths: results from the prospective, observational PURPOSe study
Authors: Harakuni Sheetal U. , Somannavar Manjunath S. , Ghanchi Najia K. , Ahmed Imran , Zafar Afia , Kim Jean , Tikmani Shiyam Sunder , Hwang Kay , Saleem Sarah , Goudar Shivaprasad S. , Dhaded Sangappa , Guruprasad Gowder , Yasmin Haleema , Yogeshkumar S. , Aceituno Anna , Silver Robert M. , McClure Elizabeth M. , Goldenberg R. L. ,   the PURPOSe Investigators
Published: April 2023

AbstractObjectiveTo examine internal organ tissues and placentas of stillbirths for various pathogens.DesignProspective, observational study.SettingsThree study hospitals in India and a large maternity hospital in Pakistan.PopulationStillborn infants delivered in a study hospital.MethodsA prospective observational study.Main outcome measuresOrganisms identified by pathogen polymerase chain reaction (PCR) in internal organs and placental tissues of stillbirths.ResultsOf 2437 stillbirth internal tissues, 8.3% (95% CI 7.2–9.4) were positive. Organisms were most commonly detected in brain (12.3%), cerebrospinal fluid (CSF) (9.5%) and whole blood (8.4%). Ureaplasma urealyticum/parvum was the organism most frequently detected in at least one internal organ (6.4% of stillbirths and 2% of all tissues). Escherichia coli/Shigella was the next most common (4.1% one or more internal organ tissue sample and 1.3% of tissue samples), followed by Staphylococcus aureus in at least one internal organ tissue (1.9% and 0.9% of all tissues). None of the other organisms was found in more than 1.4% of the tissue samples in stillbirths or more than 0.6% of the internal tissues examined. In the placenta tissue, membrane or cord blood combined, 42.8% (95% CI 40.2–45.3) had at least one organism identified, with U. urealyticum/parvum representing the most commonly identified (27.8%).ConclusionsIn about 8% of stillbirths, there was evidence of a pathogen in an internal organ. Ureaplasma urealyticum/parvum was the most common organism found in the placenta and in the internal tissues, especially in the fetal brain.
Survival in very preterm infants with congenital diaphragmatic hernia and association with prenatal imaging markers: A retrospective cohort study
Authors: Horn‐Oudshoorn Emily J. J. , Russo Francesca M. , Deprest Jan A. , Kipfmueller Florian , Geipel Annegret , Schaible Thomas , Rafat Neysan , Cordier Anne‐Gael , Benachi Alexandra , Abbasi Nimrah , Chiu Priscilla P. L. , de Boode Willem P. , Sikkel Esther , Peters Nina C. J. , Hansen Bettina E. , Reiss Irwin K. M. , DeKoninck Philip L. J. 
Published: April 2023

AbstractObjectivesTo describe the outcomes of preterm born infants with congenital diaphragmatic hernia (CDH; ≤32.0 weeks of gestation) and the associations between prenatal imaging markers and survival.DesignRetrospective cohort study.SettingMulticentre study in large referral centres.PopulationInfants with an isolated unilateral CDH, live born at 32.0 weeks or less of gestation, between January 2009 and January 2020.MethodsNeonatal outcomes were evaluated for infants that were expectantly managed during pregnancy and infants that underwent fetoscopic endoluminal tracheal occlusion (FETO) therapy, separately. We evaluated the association between prenatal imaging markers and survival to discharge. Prenatal imaging markers included observed to expected lung‐to‐head ratio (o/e LHR), side of the defect, liver position, stomach position grade, and observed to expected total fetal lung volume (o/e TFLV).Main Outcome MeasureSurvival to discharge.ResultsWe included 53 infants born at 30+4 (interquartile range 29+1–31+2) weeks. Survival in fetuses expectantly managed during pregnancy was 48% (13/27) in left‐sided CDH and 33% (2/6) in right‐sided CDH. Survival in fetuses that underwent FETO therapy was 50% (6/12) in left‐sided CDH and 25% (2/8) in right‐sided CDH. The o/e LHR at baseline was positively associated with survival in cases expectantly managed during pregnancy (odds ratio [OR] 1.20, 95% CI 1.07–1.42, p < 0.01), but not in cases that received FETO therapy (OR 1.01, 95% CI 0.88–1.15, p = 0.87). Stomach position grade (p = 0.03) and o/e TFLV were associated with survival (p = 0.02); liver position was not (p = 0.13).ConclusionsIn infants with CDH born at or before 32 weeks of gestation, prenatal imaging markers of disease severity were associated with postnatal survival.
Towards the microbial home: An overview of developments in next‐generation sustainable architecture
Authors: Armstrong Rachel 
Published: April 2023

AbstractDisruptive innovation is needed to raise the threshold of sustainable building performance, so that our buildings improve on net zero impacts and have a life‐promoting impact on the natural world. This article outlines a new approach to next‐generation sustainable architecture, which draws on the versatile metabolisms of microbes as a platform by incorporating microbial technologies and microbially produced materials into the practice of the built environment. The regenerative architecture arising from these interventions includes a broad range of advances from using new materials, to creating bioreceptive surfaces that promote life, and providing green, bio‐remediating energy from waste. Such innovations are presently reaching the marketplace as novel materials like Biocement® with lower embodied carbon than conventional materials that adopt microbially facilitated processes, and as novel utilities like PeePower® that transforms urine into electrical energy and bioreactor‐based building systems such as the pioneering BIQ building in Hamburg. While the field is still young, some of these products (e.g. mycelium biocomposites) are poised for uptake by the public–private economic axis to become mainstream within the building industry. Other developments are creating new economic opportunities for local maker communities that empower citizens and catalyse novel vernacular building practices. In particular, the activation of the microbial commons by the uptake of microbial technologies and materials through daily acts of living, ‘democratises’ resource harvesting (materials and energy) in ways that sustain life, and returns important decisions about how to run a home back to citizens. This disruptive move re‐centres the domestic‐commons economic axis to the heart of society, setting the stage for new vernacular architectures that support increasingly robust and resilient communities.
Acoustic animal identification using unsupervised learning
Authors: Guerrero Maria J. , Bedoya Carol L. , López José D. , Daza Juan M. , Isaza Claudia 
Published: April 2023

Abstract

Passive acoustic monitoring is usually presented as a complementary approach to monitoring wildlife communities and assessing ecosystem conditions. Automatic species detection methods support biodiversity monitoring and analysis by providing information on the presence–absence of species, which allows understanding the ecosystem structure. Therefore, different alternatives have been proposed to identify species. However, the algorithms are parameterized to identify specific species. Analysing multiple species would help to monitor and quantify biodiversity, as it includes the different taxonomic groups present in the soundscape.

We present an unsupervised methodology for multi‐species call recognition from ecological soundscapes. The proposal is based on a clustering algorithm, specifically the learning algorithm for multivariate data analysis (LAMDA) 3pi algorithm, which automatically suggests the number of clusters associated with the sonotypes. Emphasis was made on improving the segmentation of the audio to analyse the whole soundscape without parameterizing the algorithm according to each taxonomic group.

To estimate the performance of our proposal, we used four datasets from different locations, years and habitats. These datasets contain sounds from the four major taxonomic groups that dominate terrestrial soundscapes (birds, amphibians, mammals and insects) in audible and ultrasonic spectra. The methodology presents performances between 75% and 96% in presence–absence species recognition.

Using the clusters proposed by our methodology, the whole soundscape biodiversity was measured and compared with the estimate of four acoustic indices (ACI, NP, SO and BI). Our approach performs biodiversity assessments similar to acoustic indices with the advantage of providing information about acoustic communities without the need for prior knowledge of the species present in the audio recordings.