bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceived barriers and facilitators to geriatric trauma risk assessment: Instrument development and validation
Authors: Adeyemi Oluwaseun , Konda Sanjit , Grudzen Corita , DiMaggio Charles , Esper Garrett , Rogers Erin , Goldfeld Keith , Blecker Saul , Chodosh Joshua 
Published: June 2023

AbstractBackgroundIn the fast-paced and high-stress environment of the ED, conducting a thorough and efficient risk assessment is may be associated with various challenges such as time constraints and competing priorities. The aim of this study is to develop and validate a survey instrument that will comprehensively assesses emergency provider and nurses perceived barriers and facilitators to geriatric trauma risk assessment.MethodsWe designed two six-item survey that each assesses the barriers and facilitators to geriatric trauma risk assessment using the American College of Surgeons geriatric trauma management guidelines. Each item in the survey has a quantitative section, answered on a binary scale, and a qualitative open ended responses. Nine content experts performed content validation of the items in the scale and we computed Cohen’s Kappa, and item and scale content validity indices (CVIs).ResultsMost of the experts were male (56%), and non-Hispanic Whites (44%). A third of the content experts are MDs. Of the six items in the perceived barriers scale, five items were retained. The Cohen’s Kappa value across the five items ranged from 0.4 to 0.9 and the item and scale CVIs for the five items were 0.76 each. Of the six items in the perceived facilitators to geriatric trauma risk assessment, all six items were retained. The Cohen’s Kappa value across the six items was 1.00 and the item and scale CVIs for the six items were 1.00 each.ConclusionWe presents an instrument that can assess the perceived barriers and facilitators to geriatric trauma risk assessment experienced by emergency providers and nurses.
A modular approach to handlein-vivodrift correction for high-density extracellular recordings
Authors: Garcia Samuel , Windolf Charlie , Boussard Julien , Dichter Benjamin , Buccino Alessio P. , Yger Pierre 
Published: June 2023

AbstractHigh-density neural devices are now offering the possibility to record from neuronal populationsin-vivoat unprecedented scale. However, the mechanical drifts often observed in these recordings are currently a major issue for “spike sorting”, an essential analysis step to identify the activity of single neurons from extracellular signals. Although several strategies have been proposed to compensate for such drifts, the lack of proper benchmarks makes it hard to assess the quality and effectiveness of motion correction. In this paper, we present an exhaustive benchmark study to precisely and quantitatively evaluate the performance of several state-of-the-art motion correction algorithms introduced in literature. Using simulated recordings with induced drifts, we dissect the origins of the errors performed while applying motion-correction algorithm as a preprocessing step in the spike sorting pipeline. We show how important it is to properly estimate the positions of the neurons from extracellular traces in order to correctly estimate the probe motion, compare several interpolation procedures, and highlight what are the current limits for motion correction approaches.Significance statement
Mechanistic constraints on the trade-off between photosynthesis and respiration in response to warming
Authors: Leles Suzana G. , Levine Naomi M. 
Published: June 2023

AbstractPhytoplankton are responsible for half of all oxygen production and drive the ocean carbon cycle. Metabolic theory predicts that increasing global temperatures will cause phytoplankton to become more heterotrophic and smaller. Here we uncover the metabolic trade-offs between cellular space, energy, and stress management driving phytoplankton thermal acclimation and how these might be overcome through evolutionary adaptation. We show that the observed relationships between traits such as chlorophyll, lipid content, C:N and size can be predicted based on the metabolic demands of the cell, the thermal dependency of transporters, and changes in membrane lipids. We suggest that many of the observed relationships are not fixed physiological constraints but rather can be altered through adaptation. For example, the evolution of lipid metabolism can favor larger cells with higher lipid content to mitigate oxidative stress. These results have implications for rates of carbon sequestration and export in a warmer ocean.TeaserA tale of how photosynthetic microbes might defy current trends to become larger and grow faster in a warmer ocean.
Characterization of a uranium-tolerant green microalga of the genusCoelastrellawith high potential for the remediation of metal-polluted waters
Authors: Beaulier Camille , Dannay Marie , Devime Fabienne , Baggio Célia , Sakkout Nabila El , Raillon Camille , Courson Olivier , Bourguignon Jacques , Alban Claude , Ravanel Stéphane 
Published: June 2023

AbstractUranium (U) pollution of terrestrial and aquatic ecosystems poses a significant threat to the environment and human health because this radionuclide is chemotoxic. Characterization of organisms that tolerate and accumulate U is critical to decipher the mechanisms evolved to cope with the radionuclide and to propose new effective strategies for bioremediation of U-contaminated environments. Here, we isolated a unicellular green microalga of the genusCoelastrellafrom U-contaminated wastewater. We showed thatCoelastrellasp. PCV is much more tolerant to U thanChlamydomonas reinhardtiiandChlorella vulgaris.Coelastrellais able to accumulate U very rapidly, then gradually release it into the medium, behaving as an excluder to limit the toxic effects of U. The ability ofCoelastrellato accumulate U is remarkably high, with up to 600 mg U sorbed per g dry biomass.Coelastrellais able to grow and maintain high photosynthesis in natural metal-contaminated waters from a wetland near a reclaimed U mine. Over a single one-week growth cycle,Coelastrellais able to capture 25-55% of U from contaminated waters and demonstrates lipid droplet accumulation.Coelastrellasp. PCV is a very promising microalga for the remediation of polluted waters with valorization of algal biomass that accumulates lipids.Graphical abstract
The catalytic-independent function of LSD1 modulates the epigenetic landscape of mouse embryonic stem cells
Authors: Malla Sandhya , Kumari Kanchan , Martinez-Gamero Carlos , García-Prieto Carlos A. , Stransky Stephanie , Caroli Jonatan , Álvarez-Errico Damiana , Saiki Paulina Avovome , Lai Weiyi , Lyu Cong , Gilthorpe Jonathan D. , Wang Hailin , Sidoli Simone , Mattevi Andrea , Mateus Andre , Esteller Manel , Roman Angel , Aguilo Francesca 
Published: June 2023

AbstractLysine-specific histone demethylase 1 (LSD1), which demethylates mono- or di-methylated histone H3 on lysine 4 (H3K4me1/2), is essential for early embryogenesis and development. Here we show that LSD1 is dispensable for embryonic stem cell (ESC) self-renewal but is required for ESC growth and differentiation. Reexpression of a catalytically-dead LSD1 (LSD1MUT) recovers the proliferation capability of ESCs, yet the enzymatic activity of LSD1 is essential to ensure proper differentiation. Indeed, a gain of H3K4me1 inLsd1knockout (KO) ESCs does not lead to major changes in global gene expression programs related to stemness. However, ablation of LSD1 but not LSD1MUTresults in decreased DNMT1 and UHRF1 proteins coupled to global hypomethylation. We show that both LSD1 and LSD1MUTcontrol protein stability of UHRF1 and DNMT1 through interaction with the ubiquitin-specific peptidase 7 (USP7) and, consequently, inhibiting DNMT1 and UHRF1 ubiquitylation. Our studies elucidate for the first time a novel mechanism by which the scaffolding function of LSD1 controls DNA methylation in ESCs.
High genomic plasticity and unique features ofXanthomonas translucenspv.graminisrevealed through comparative analysis of complete genome sequences
Authors: Goettelmann Florian , Koebnik Ralf , Roman-Reyna Veronica , Studer Bruno , Kölliker Roland 
Published: June 2023

AbstractBackgroundXanthomonas translucenspv.graminis(Xtg) is a major bacterial pathogen of economically important forage grasses, causing severe yield losses. So far, genomic resources for this pathovar consisted mostly of draft genome sequences, and only one complete genome sequence was available, preventing comprehensive comparative genomic analyses. Such comparative analyses are essential in understanding the mechanisms involved in the virulence of pathogens and to identify virulence factors involved in pathogenicity.ResultsIn this study, we produced high-quality, complete genome sequences of four strains ofXtg, complementing the recently obtained complete genome sequence of theXtgpathotype strain. These genomic resources allowed for a comprehensive comparative analysis, which revealed a high genomic plasticity with many chromosomal rearrangements, although the strains were highly related, with 99.9 to 100% average nucleotide identity. A high number of transposases were exclusively found inXtgand corresponded to 413 to 457 insertion/excision transposable elements per strain. These mobile genetic elements are likely to be involved in the observed genomic plasticity and may play an important role in the adaptation ofXtg. The pathovar was found to lack a type IV secretion system, and it possessed the smallest set of type III effectors in the species. However, three XopE and XopX family effectors were found, while in the other pathovars of the species two or less were present. Additional genes that were specific to the pathovar were identified, including a unique set of minor pilins of the type IV pilus, 17 TonB-dependent receptors (TBDRs), and 11 degradative enzymes.ConclusionThese results suggest a high adaptability ofXtg, conferred by the abundance of mobile genetic elements, which may have led to the loss of many features. Conserved features that were specific toXtgwere identified, and further investigation will help to determine genes that are essential to pathogenicity and host adaptation ofXtg.
A one-track model for spatiotemporal coordination ofBacillus subtilisseptal cell wall synthesis
Authors: Whitley Kevin D. , Grimshaw James , Roberts David M. , Karinou Eleni , Stansfeld Phillip J. , Holden Séamus 
Published: June 2023

AbstractBacterial cell division requires synthesis of a septal peptidoglycan (sPG) wall across the middle of the cell. This is accomplished by the divisome synthesis complex in coordination with numerous other division proteins—such as the essential tubulin homolog FtsZ—but the molecular mechanism of its spatiotemporal regulation remains unclear. Here, we investigate the dynamics of sPG synthesis in the model Gram-positive bacteriumBacillus subtilisusing live-cell single-molecule imaging of the divisome transpeptidase PBP2B. In contrast to previous models for division, we show that there is a single population of processively-moving PBP2B molecules whose motion is driven by peptidoglycan synthesis and is not associated with FtsZ treadmilling. However, although the motions of PBP2B and FtsZ are asynchronous, we demonstrate that processive PBP2B motion is partially dependent on FtsZ treadmilling. Additionally, we provide evidence that the divisome synthesis complex is multimeric. Our results support a new model for division inB. subtiliswhere a multimeric synthesis complex follows a single track dependent on sPG synthesis whose activity and dynamics are asynchronous with FtsZ treadmilling.
Post-Transplant Administration of G-CSF Impedes Engraftment of Gene Edited Human Hematopoietic Stem Cells by Exacerbating the p53-Mediated DNA Damage Response
Authors: Araki Daisuke , Chen Vicky , Redekar Neelam , Salisbury-Ruf Christi , Luo Yan , Liu Poching , Li Yuesheng , Smith Richard H. , Dagur Pradeep , Combs Christian , Larochelle Andre 
Published: June 2023

AbstractGranulocyte colony stimulating factor (G-CSF) is commonly used as adjunct treatment to hasten recovery from neutropenia following chemotherapy and autologous transplantation of hematopoietic stem and progenitor cells (HSPCs) for malignant disorders. However, the utility of G-CSF administration afterex vivogene therapy procedures targeting human HSPCs has not been thoroughly evaluated. Here, we provide evidence that post-transplant administration of G-CSF impedes engraftment of CRISPR-Cas9 gene edited human HSPCs in xenograft models. G-CSF acts by exacerbating the p53-mediated DNA damage response triggered by Cas9- mediated DNA double-stranded breaks. Transient p53 inhibition in culture attenuates the negative impact of G-CSF on gene edited HSPC function. In contrast, post-transplant administration of G-CSF does not impair the repopulating properties of unmanipulated human HSPCs or HSPCs genetically engineered by transduction with lentiviral vectors. The potential for post-transplant G-CSF administration to aggravate HSPC toxicity associated with CRISPR-Cas9 gene editing should be considered in the design ofex vivoautologous HSPC gene editing clinical trials.Graphical Abstract
Inflammatory macrophages prevent colonic goblet and enteroendocrine cell differentiation through Notch signaling
Authors: Atanga Roger , Romero Aaron S. , Hernandez Anthony Jimenez , Peralta-Herrera Eduardo , Merkley Seth D. , In Julie G. , Castillo Eliseo F. 
Published: June 2023

AbstractInflammatory macrophages in the intestine are a key pathogenic factor driving inflammatory bowel disease (IBD). Here, we report the role of inflammatory macrophage-mediated notch signaling on secretory lineage differentiation in the intestinal epithelium. Utilizing IL-10-deficient (Il10-/-) mice, a model of spontaneous colitis, we found an increase in Notch activity in the colonic epithelium as well as an increase in intestinal macrophages expressing Notch ligands, which are increased in macrophages upon inflammatory stimuli. Furthermore, a co-culture system of inflammatory macrophages and intestinal stem and proliferative cells during differentiation reduced goblet and enteroendocrine cells. This was recapitulated when utilizing a Notch agonist on human colonic organoids (colonoids). In summary, our findings indicate that inflammatory macrophages upregulate notch ligands that activate notch signaling in ISC via cell-cell interactions, which in turn inhibits secretory lineage differentiation in the gastrointestinal (GI) tract.
The evolutionary history of hepaciviruses
Authors: Li YQ , Ghafari M , Holbrook AJ , Boonen I , Amor N , Catalano S , Webster JP , Li YY , Li HT , Vergote V , Maes P , Chong YL , Laudisoit A , Baelo P , Ngoy S , Mbalitini SG , Gembu GC , Akawa P Musaba , Goüy de Bellocq J , Leirs H , Verheyen E , Pybus OG , Katzourakis A , Alagaili AN , Gryseels S , Li YC , Suchard MA , Bletsa M , Lemey P 
Published: June 2023

AbstractIn the search for natural reservoirs of hepatitis C virus (HCV), a broad diversity of non-human viruses within theHepacivirusgenus has been uncovered. However, the evolutionary dynamics that shaped the diversity and timescale of hepaciviruses evolution remain elusive. To gain further insights into the origins and evolution of this genus, we screened a large dataset of wild mammal samples (n =1,672) from Africa and Asia, and generated 34 full-length hepacivirus genomes. Phylogenetic analysis of these data together with publicly available genomes emphasizes the importance of rodents as hepacivirus hosts and we identify 13 rodent species and 3 rodent genera (in Cricetidae and Muridae families) as novel hosts of hepaciviruses. Through co-phylogenetic analyses, we demonstrate that hepacivirus diversity has been affected by cross-species transmission events against the backdrop of detectable signal of virus-host co-divergence in the deep evolutionary history. Using a Bayesian phylogenetic multidimensional scaling approach, we explore the extent to which host relatedness and geographic distances have structured present-day hepacivirus diversity. Our results provide evidence for a substantial structuring of mammalian hepacivirus diversity by host as well as geography, with a somewhat more irregular diffusion process in geographic space. Finally, using a mechanistic model that accounts for substitution saturation, we provide the first formal estimates of the timescale of hepacivirus evolution and estimate the origin of the genus to be about 22 million years ago. Our results offer a comprehensive overview of the micro- and macroevolutionary processes that have shaped hepacivirus diversity and enhance our understanding of the long-term evolution of theHepacivirusgenus.SignificanceSince the discovery of Hepatitis C virus, the search for animal virus homologues has gained significant traction, opening up new opportunities to study their origins and long-term evolutionary dynamics. Capitalizing on a large-scale screening of wild mammals, and genomic sequencing, we expand the novel rodent host range of hepaciviruses and document further virus diversity. We infer a significant influence of frequent cross-species transmission as well as some signal for virus-host co-divergence, and find comparative host and geographic structure. We also provide the first formal estimates of the timescale of hepaciviruses indicating an origin of about 22 million years ago. Our study offers new insights in hepacivirus evolutionary dynamics with broadly applicable methods that can support future research in virus evolution.
Technostress and information and communication technology usage among librarians in Nigerian universities
Authors: Olorunfemi Michael , Adekoya Clement Ola 
Published: June 2023

Purpose
This paper aims to investigate technostress and information and communication technology (ICT) usage among librarians in Nigerian universities.

Design/methodology/approach
Descriptive survey research design was used for the study. Questionnaire was used for data collection. The data generated were analyzed using mean, frequency count and percentage.

Findings
A moderate extent of psychological, emotional and physical technostress was found among the librarians. The findings further revealed that technostress among the librarians could be reduced through organizational and technical support and the involvement of librarians in the ICT implementation process. The hypotheses tested revealed that there was a significant relationship between technostress and ICT usage among the librarians; female librarians exhibit a higher extent of technostress than male librarians, and there is no difference in the extent of ICT usage between male and female librarians in Nigerian universities.

Practical implications
Librarians cannot effectively use ICTs if they exhibit technostress.

Originality/value
The work is a creative attempt to know the difference in the extent of technostress and ICT usage among male and female librarians in Nigerian universities.
How do firms obtain benefits from quality awards: An empirical study of the winners of the China quality award
Authors: Li Xingxi , Wu Su , Liu Huiming 
Published: June 2023

PurposeQuality awards are widely considered symbols of successful quality management. The purpose of this paper is to empirically test the effects of the China Quality Award (CQA) on firms' performance. The study further explores how the benefits due to CQA are affected by contextual factors.Design/methodology/approachUsing the data of CQA winners from 2001 to 2016, the event study method is applied to analyze the abnormal performance of winners. Furthermore, multiple regression models are proposed to evaluate the effects of contextual factors on the relationship between the award and profitability.FindingsThe findings show that CQA has positive impacts on profitability and fixed asset efficiency but not on labor productivity. Besides, state-owned firms and firms with high innovation intensity obtain more profitability benefits than others.Originality/valueThis is the first study to explain the relationship between quality awards and firm performance from a theoretical perspective, providing new insights into the quality management and performance literature. Furthermore, this study deepens the understanding of the relationship between quality awards and performance and reveals new implications. Some of the contextual factors examined, such as innovation intensity, are considered for the first time in quality award research.
A new quality measure for acceptance and payment with a QC/QA performance specification using Bayesian updating
Authors: Sullivan James L. , Novak David , Hernandez Eric , Van Den Berg Nick 
Published: June 2023

PurposeThis paper introduces a novel quality measure, the percent-within-distribution, or PWD, for acceptance and payment in a quality control/quality assurance (QC/QA) performance specification (PS).Design/methodology/approachThe new quality measure takes any sample size or distribution and uses a Bayesian updating process to re-estimate parameters of a design distribution as sample observations are fed through the algorithm. This methodology can be employed in a wide range of applications, but the authors demonstrate the use of the measure for a QC/QA PS with upper and lower bounds on 28-day compressive strength of in-place concrete for bridge decks.FindingsThe authors demonstrate the use of this new quality measure to illustrate how it addresses the shortcomings of the percent-within-limits (PWL), which is the current industry standard quality measure. The authors then use the PWD to develop initial pay factors through simulation regimes. The PWD is shown to function better than the PWL with realistic sample lots simulated to represent a variety of industry responses to a new QC/QA PS.Originality/valueThe analytical contribution of this work is the introduction of the new quality measure. However, the practical and managerial contributions of this work are of equal significance.
Leadership and communication: how to assess executive skills
Authors: Biggs Adam , Johnston Scott , Russell Dale 
Published: June 2023

Purpose
Leadership assessment programs are intended to ensure that the organization retains or hires high-quality leadership. Among the many skills that must be included, executive communication is a cornerstone of effective leadership. However, there are many techniques to assessing executive communication that impose numerous advantages and disadvantages. The purpose of this study is to explore several techniques for evaluating executive communication skills in leadership assessment programs.

Design/methodology/approach
Building upon case studies from novel commanding officer selection efforts in the military, the current study outlines three possible areas of executive communication for leadership assessment programs: panel-based interviews, guided discussion and executive writing.

Findings
Although each technique offers some advantages, the best technique depends upon the context. Panel-based interviews can provide excellent depth in evaluating candidates, whereas executive writing focuses more upon crafting a deliberate and clear message without the ability to clarify or use nonverbal cues. Selecting an appropriate technique depends greatly upon the workload imposed on the leadership assessment team and the number of candidates available.

Originality/value
Leadership selection programs are often done piecemeal or based on local experience. By building upon novel efforts in military commanding officer selection, the goal is to promulgate effective executive communication techniques that will enhance leadership selection through more effective communication across all levels of leadership positions.