bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnosis of colorectal cancer based on folate receptor-positive circulating tumor cell analysis: a retrospective cohort study
Authors: Wei Zhijian , Zhou Baichuan , Wang Guoxing , Han Wenxiu , Li Yongxiang , Xu Aman 
Published: June 2023

Abstract
Background
Early diagnosis and treatment are crucial to improve the prognosis of colorectal cancer (CRC). At present, there is a lack of an accurate CRC screening factor. We conducted folate receptor-positive circulating tumor cell analysis (FR + CTC analysis) in distinguishing CRC from benign colorectal diseases to evaluate the diagnostic efficiency.
Methods
Clinical data of patients admitted to The First Affiliated Hospital of Anhui Medical University from January 2021 to July 2022 were retrospectively collected. Levels of FR + CTC and other indicators were analyzed. Receiver operating characteristic (ROC) analysis was performed to assess the diagnostic performance of these molecular biomarkers.
Results
Data of 103 patients with CRC and 54 patients with benign colorectal diseases were collected. FR + CTC levels were observed significantly higher in CRC patients than in patients with benign colorectal diseases (P < 0.001). FR + CTC level was correlated with tumor diameter, differentiation, T-stage, pathological stage, clinical stage, and intravascular tumor thrombus in patients with CRC (P < 0.05). The optimal cutoff value of FR + CTC level for diagnosing CRC patients was 7.66 FU/3 ml, with a sensitivity of 85.4%, a specificity of 74.1%, and an Area Under Curve (AUC) of 0.855 (95%CI, 0.77–0.923). In < 50-years old patients with CRC, the diagnostic efficiency of FR + CTC was excellent, with an AUC of 0.936 (95%CI, 0.877–0.995).
Conclusion
FR + CTC counting has excellent diagnostic efficiency in screening of CRC. FR + CTC count can also predict the tumor stage of CRC patients before surgery, and guide the choice of treatment.
Effects of INF-γ-modified bone marrow mesenchymal stem cells on the distribution and inhibition of tumours in vivo in a glioma nude mouse model
Authors: Xuejiao Tian , Sunhu Yang , Yanying Zhang , Yindi Wang , Zhen Lv , Yahui Xie , Xiangning Xu , Yihong Tian , Jianjun Wu , Jianjun Wu 
Published: June 2023

Abstract
Explored the effects of INF-γ-modified bone marrow mesenchymal stem cells (BMSCs) on the distribution and inhibition of tumour tissues. BALB/c mouse BMSCs were cultured, isolated, and identified by flow cytometry. To confirm INF-γ expression in the BMSCs, we constructed a lentiviral expression vector for the INF-γgene; BMSCs transfected in vitro were labelled with SYBR Green I fluorescent dye and detected by fluorescent quantitative polymerase chain reaction (qPCR). Tumour tissues of nude mice were subjected to terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining, and the number of positive cells was determined. The result that BMSCs were cultured in vivo and exhibited adherent growth. Flow cytometry indicated that CD44 (98.01%) and CD105 (96.17%) were overexpressed, whereas the CD34 (1.46%), CD45 (1.32%), and CD11b (1.48%) expression levels were low, indicating that the latter were BMSCs. Fluorescence analysis and PCR were applied to confirm INF-γtransfection into the BMSCs. Immunofluorescence staining showed clear accumulation of BMSCs in nude mouse tissues, with no fluorescence observed in the model group. TUNEL staining showed a higher apoptosis rate in the INF-γ+BMSC group than in the model group (P < 0.05). On day 9, the tumour volume differed significantly between the INF-γ+BMSC group and the other groups (P < 0.05). A lentiviral vector effectively transfected the INF-γ gene into BMSCs, where it was homed and distributed to tumour tissues, significantly inhibiting tumour growth.
Innovative Environment-Friendly liquid fertilizer bead from sodium alginate coating with IPN membrane derived from Natural rubber and Cassava starch
Authors: Phansroy Nichanan , Boonyod Saowaluk , Mulasake Oanchali , Uttha Apinya , Songkram Channarong , Somboon Theerasap , Kongon Jakkrawut , Lersuwannapong Niwat , Saengsuwan Sayant , Khawdas Wichean , Vudjung Chaiwute 
Published: June 2023

Abstract

This research aims to prepare liquid fertilizer beads formed with sodium alginate (SA) (SAB) that were coated with interpenetrating polymer network (IPN) hydrogels based on pre-vulcanized natural rubber latex (NR) and cassava starch (St) (IPN NR/St) for controlling the release of the urea solutions. The preparation of SAB at various concentrations of SA and calcium chloride (CaCl2) solutions was investigated. It was found that the appropriate concentrations of SA and CaCl2 solutions were 1.5 and 5.0 wt%, respectively. The obtained SAB was coated with IPN NR/St, the ratio between SAB and IPN NR/St at 1.0/1.0, and the number of coated layers of IPN NR/St at 2 layers. As a result, coated alginate beads (CSAB) can decelerate the release of urea solution, and enhance the Thai eggplant growth. Thus, the CSAB could enhance not only the release control of urea solution but also lesser environmental problems, and is a novel technique for coating and the liquid release control for organic or biofertilizers.
Heavy Metal Estimation in Surface Sediments of Ujani Reservoir, North West of India
Authors: Ghule Yuvraj A. , Gavali Ravindra S , John Nissy ,   4.	Goundla Pradeep Goud
Published: June 2023

Abstract

The current study was focused to assess the sediment contamination of heavy metals (As, Cd, Cr, Pb, Hg, Ti) collected from Ujani Reservoir, a tributary of Krishna River located in the industrialized region of Solapur District, Maharashtra. Samples were detected to check the potential contamination status and analyze the degree of usability of the water for domestic purposes. Assessment of seasonal variations of the metal concentrations in the sediments during the pre- monsoon and post-monsoon months showed no significant difference. Sediment contamination was calculated on the basis of Pollution Load Index (PLI), Geoaccumulation Index (Igeo), Contamination Factor (CF) and Potential Ecological Risk Index/ (PERI). The results analyzed by Principal Component Analysis (PCA) confirm that Chromium (Cr) and Mercury (Hg) were the major contaminants in the river which indicates direct input of wastes from agriculture and petroleum industries. The outcomes of the results demonstrated that the average value of two recognized heavy metals (Cr), and (Hg), exceeded the safe limits of drinking water, warning the consumption of water from the river is unsafe for domestic purposes. The high concentrations of the metals in the river water may be attributed to the sediment deposition due to man induced activities. These findings may help explicitly to develop new river management strategies and control heavy metal pollution to prevent further damage to fresh water bodies
Mesocosm experiments of temperate marine coastal waters: timing of changes in phytoplankton communities and attachment to plastic plates after nutrient addition
Authors: Lee Chung Hyeon , Lim Young Kyun , Yoon Ji Nam , Baek Seung Ho 
Published: June 2023

Abstract
Anthropogenic pollutants, such as excessive nutrients and marine plastic debris (MPD), can significantly affect marine ecosystems, but the timing and combined effects of these pollutants are poorly understand. Nutrient enrichment commonly occurs in temperate marine coastal waters during autumn due to terrestrial runoff. We established six 1000-L mesocosms and performed experiments for 15 days during November to examine the effects of nutrient enrichment and MPD. The mesocosms (2 replicates per group) had natural seawater (control), low nutrient (LN) seawater, or high nutrient (HN) seawater with polypropylene (PP) plates to mimic the effect of MPD. We recorded the timing of changes in nutrients and other abiotic factors and in phytoplankton in the water column and in the periphyton communities on the PP plates. Cryptomonas spp. was initially dominant in all three groups, but the LN and HN groups shifted to chain diatoms, such as Pseudo-nitzschia spp., and then to Cylindrotheca closterium. Due to significant nutrient uptake by bloom-forming diatoms, most of the nitrate+nitrite and phosphate were consumed within 4 days in the LN group. In contrast, the some nutrients remained in the HN group until day 15, and this was related to the nutrient requirements of other small solitary diatoms, such as C. closterium. Analysis of the PP plates showed that although small numbers of the planktonic diatom Chaetocerosspp. were present from day 4, there was a shift to the attached diatom C. closterium during the middle and end of the experiment, indicating that C. closterium in the water attached to the PP plates even when nutrients levels were low. In addition, Navicula(>20%) was prominent on the PP plates from the middle to the end of the experiment. Notably, the percentage of total Chl. a in the periphyton community, which was associated with nutrient uptake in the HN group, was higher than in the phytoplankton community. This implies that the periphyton biomass in the HN group effectively utilized the remaining nutrients. Our mesocosm experiments provide important ecological insights regarding the timing of changes of phytoplankton in the water column and of microalgae attached to MPD when nutrients and plastic debris are introduced into coastal waters through river discharge after rainfall during autumn in temperate regions.
Potential of computational models in personalized treatment of obstructive sleep apnea: a patient-specific 3-d finite element study
Authors: Ayyalasomayajula Venkat , Moxeness Mads H S , Skallerud Bjørn 
Published: June 2023

Abstract
The upper airway experiences mechanical loads during breathing. Obstructive sleep apnea is a very common sleep disorder, in which the normal function of the airway is compromised, enabling its collapse. Its treatment remains unsatisfactory with variable efficacy in the case of many surgeries. Finite element models of the upper airway to simulate the effects of various anatomic and physiologic manipulations on its mechanics could be helpful in predicting surgical success. Partial 3-D finite element models based on patient-specific CT-scans are generated. The pharyngeal wall, the soft palate, the tongue, and their interactions are aimed at. Anatomic and physiologic operations are then conducted on the generated models to reflect commonly employed surgical techniques. Our results showed that maxillo-mandibular advancement(MAD) surgery of just 1 cm improved the critical closing pressure by atleast 212.2%. Following MAD, the best improvement was seen via uvulopalatopharyngoplasty (UPPP), with an improvement of at least 19.12%. Palatal and tongue implants also offered a certain degree of improvement. Further, we observed possible interacting mechanisms which suggested simultaneous implementation of UPPP and tongue stiffening; and palatal and tongue stiffening could be beneficial. Our results suggest that computational modeling is a useful tool for analyzing the influence of anatomic and physiological manipulations on upper airway mechanics. The goal of personalized treatment in the case of OSA could be achieved with the use of computational modeling.
Peripheral MC1R activation modulates immune responses and is neuroprotective in a mouse model of Parkinson's disease
Authors: Srivast Pranay , Nishiyama Shuhei , Lin Sonia H , Srivast Akriti , Su Chienwen , Peng Weiyi , Levy Michael , Schwarzschild Michael , Xu Yuehang , Chen Xiqun 
Published: June 2023

Abstract
Background: Melanocortin 1 receptor (MC1R) is a key pigmentation gene, and loss-of-function of MC1R variants that produce red hair may be associated with Parkinson’s disease (PD). We previously reported compromised dopaminergic neuron survival in Mc1r mutant mice and dopaminergic neuroprotective effects of local injection of a MC1R agonist to the brain or a systemically administered MC1R agonist with appreciable CNS permeability. Beyond melanocytes and dopaminergic neurons, MC1R is expressed in other peripheral tissues and cell types, including immune cells. The present study investigates the impact of NDP-MSH, a synthetic melanocortin receptor (MCR) agonist that does not cross BBB, on the immune system and the nigrostriatal dopaminergic system in mouse model of PD.
Methods: C57BL/6 mice were treated systemically with MPTP.HCl (20 mg/kg) and LPS (1 mg/kg) from day 1 to day 4 and NDP-MSH (400 µg/kg) or vehicle from day 1 to day 12 following which the mice were sacrificed. Peripheral and CNS immune cells were phenotyped and inflammatory markers were measured. The nigrostriatal dopaminergic system was assessed behaviorally, chemically, immunologically, and pathologically. To understand the role of regulatory T cells (Tregs) in this model, CD25 monoclonal antibody was used to deplete CD25+ Tregs.
Results: Systemic NDP-MSH administration significantly attenuated striatal dopamine depletion and nigral dopaminergic neuron loss induced by MPTP+LPS. It improved the behavioral outcomes in the pole test. Mc1r mutant mice injected with NDP-MSH in the MPTP and LPS paradigm showed no changes in striatal dopamine levels suggesting that the NDP-MSH acts through the MC1R pathway. Although no NDP-MSH was detected in the brain, peripheral, NDP-MSH attenuated neuroinflammation as observed by diminished microglial activation in the nigral region, along with reduced TNF-α and IL1β levels in the ventral midbrain. Depletion of Tregs limited the neuroprotective effects of NDP-MSH.
Conclusions: Our study demonstrates that peripherally acting NDP-MSH confers protection on dopaminergic nigrostriatal neurons and reduces hyperactivated microglia. NDP-MSH modulates peripheral immune responses, and Tregs may be involved in the neuroprotective effect of NDP-MSH.
Serum levels of prostate specific antigen (PSA) after primary vaccination with BNT162b2
Authors: De Nitto Simone , Pighi Laura , Salvagno Gian Luca , Lippi Giuseppe 
Published: June 2023

Abstract
We retrospectively analyzed 37 male healthy employees (mean age: 61±8 years) of the Pederzoli Hospital in Peschiera del Garda (Verona, Italy) who completed a primary vaccination cycle with the COVID-19 vaccine BNT162b2. Blood samples were drawn by standard venipuncture immediately before the first BNT162b2 dose, 21 days thereafter and thus immediately before the second BNT162b2 dose, and 1 month after the second vaccine dose (i.e., 50 days after the first BNT162b2 dose). Serum PSA levels were not significantly different from baseline (median: 0.9 ng/mL; IQR: 0.61-1.88 ng/mL) after the first BNT162b2 dose (median: 0.7 ng/mL; IQR: 0.48-1.26 ng/mL; p=0.074), and were even lower than at baseline after the second BNT162b2 dose (median: 0.7 ng/mL; IQR: 0.39-1.29 ng/mL; p=0.001). Our results suggest that the BNT162b2 vaccine may also have a beneficial effect on prostate biology and function, by reducing circulating PSA levels.
Utilizing YOLO 8x Models, Deep Learning-Based Head protector Detection for Construction Workers
Authors: Raja M. Chithik 
Published: June 2023

Abstract
Many organizations in the sector are becoming increasingly concerned about the safety of construction workers on project sites. At construction sites, safety helmets can help reduce worker injuries, but for a variety of reasons, they are frequently not worn correctly. Therefore, it is essential to have an autonomous safety helmet detection system that uses computer vision. The You Only Look Once (YOLO) principle of real-time computer vision is the foundation of this research poster. After power-law transformation, image rescaling was utilized as the initial stage in image improvement. The YOLOv8 object detection approach was used in the design of a computer vision system to determine whether or not employees were wearing helmets. Our research showed that the YOLOv8x architecture's 95.46% mean average precision (mAP) produced great results in identifying objects. Our experimental findings revealed that 95.46% of the mean average precision (mAP) in YOLOv8x architecture demonstrated excellent results in recognizing safety helmets even in low-light situations.
Correcting systematic bias in derived hydrologic simulations – Implications for climate change assessments
Authors: Sharma Ashish , Mehrotra Rajeshwar , Kusumastuti Cilcia 
Published: June 2023

Abstract
Quantifying climate change impact on water resources systems at regional or catchment scales is important in water resources planning and management. General circulation models (GCMs) represent our main source of knowledge about future climate change. However, several key limitations restrict the direct use of GCM simulations for water resource assessments. In particular, the presence of systematic bias and the need for its correction is an essential pre-processing step that improves the quality of GCM simulations, making climate change impact assessments more robust and believable. What exactly is systematic bias? Can systematic bias be quantified if the model is asynchronous with observations or other model simulations? Should model bias be sub-categorized to focus on individual attributes of interest or aggregated to focus on lower moments alone? How would one address bias in multiple attributes without making the correction model complex? How could one be confident that corrected simulations for the yet-to-be-seen future bear a closer resemblance to the truth? How can one meaningfully extrapolate correction to multiple dimensions, without being impacted by the ‘Curse of Dimensionality’? These are some of the questions we attempt to address in the paper.
Assessment of dredging impact on hydrodynamics of Surma River using hydrodynamic model: HEC-RAS approach
Authors: Rony Pritam Karmakar , Uddin Md. Misbah , Amin Gazi Md. Riasat , Munna Dr Gulam Md. , Dibya Dheeman Das 
Published: June 2023

Abstract

Surma River is the main source of fresh water in the South-Eastern region of Bangladesh. Every year, huge amounts of sediment loads are coming from upstream, which are settling down on the Surma River bed, hindering the safe passage of flow that contributes to the change in the hydrodynamic of the river. Recently, dredging has been done in the Sunamganj area to increase the navigability of the river. In this study, the hydrodynamics of the Surma River has been investigated to check the impact of dredging with the help of HEC-RAS. Flood frequency analyses have been done for 5, 10, 25, 50, and 100 years return periods, and a comparison of water level, velocity, and discharge has been done before and after dredging. Before dredging the water level, the flow path was very high, and velocity was low, but after dredging the water level, the flow path decreases and velocity increased. Discharge also decreases after dredging in the six stations, which are located in the dredged area, and the values are 1,375.41, 1,374.31, 1,373.73, 1,369.7, 1,373.11, and 1,372.08 m3/s. Before dredging, the values are 1,669.62, 1,665.53, 1,664.69, 1,658.12, 1,624.23, and 1,383.98 m3/s.
Bamboo sawdust-derived high surface area activated carbon for remarkable removal of paracetamol from aqueous solution: sorption kinetics, isotherm, thermodynamics, and regeneration studies
Authors: Wakejo Wondimu Kebede , Meshesha Beteley Tekola , Kang Joon Wun , Demesa Abayneh Getachew 
Published: June 2023

Abstract

Due to its widespread consumption, paracetamol (PCT) has emerged as one of the leading contaminants that pollute water. Herein, a PCT removal of 99.6% was achieved using chemically activated carbon (CAC), derived from bamboo sawdust using KOH/FeCl3 as an activating agent, at optimal conditions of PCT (20 mg/L), CAC (0.5 g/L), contact time (90 min), and pH (8). Kinetic study revealed that the PCT adsorption process followed the pseudo-second-order kinetic model (R2 = 0.99), indicating that chemical adsorption dominated the adsorption mechanism. On the other hand, isotherm experimental data were best described by the Langmuir (R2 = 0.98) and Freundlich (R2 = 0.96) models. CAC had a maximum Langmuir monolayer capacity of 188.67 mg/g at a PCT concentration of 120 mg/L. Moreover, the Redlich–Peterson model gave the best fit (R2 = 0.99) to the experimental data, confirming that PCT adsorption was monolayer adsorption onto the heterogeneous surface. Thermodynamically, the PCT adsorption was exothermic, spontaneous, and favorable. The reusability study depicted that CAC can be successfully reused for five consecutive adsorption–desorption cycles. Furthermore, the application of CAC to environmental samples showed interesting results. The overall adsorption study indicated that CAC could serve as a promising adsorbent for eliminating PCT from water.
Recent Developments and Future Prospects of Natural Antimicrobial
Bioactive Metabolites Obtained from Endophytic Bacterial Isolate
Authors: Lal Moti , Neelam  
Published: June 2023

Background:
Adathoda beddomei (Adosa), a kind of softwood, evergreen, perennial
shrub, has been used as a source of endophyte bacteria. Adhatoda beddomei has a wide variety of
chemicals, including anthocyanins, aminophylline, alkaloids, cardiac glycosides, isoprenaline,
triterpenoids, resins, flavonoids, tannins, sterol, saponins, etc. The root, stem, and leaf parts of Adathoda
beddomei are most often used in indigenous medicine. Moreover, the root bark is also used to
cure several conditions, including leprosy, fever, and bleeding.

Objective:
Some important bioactive metabolites were obtained from endophytes bacteria and analyzed
through various techniques (NMR, MASS, FTIR, HPLC, and UV- spectrophotometer) for
their bioactive secondary metabolites.

Methods:
In silico calculation was performed to reveal bioactive metabolites with the potential to
be antibacterial, and their primary mode of action may include dissolving bacterial and fungal cell
walls.

Results:
The antimicrobial activity of Adathoda beddomei was demonstrated against different pathogenic
and non-pathogenic bacteria. Identification of endophytes was done based on external morphological
characteristics with the help of a scanning electron microscope (SEM).

Conclusion:
Natural compounds derived from endophyte bacteria with a very low molecular mass can
be used to discover new and important structures for different pharmaceuticals and agrochemicals.
CircFAT1 Promotes the Proliferation and Invasion of Malignant Melanoma through
miR375-SLC7A11 Signal Axis
Authors: Lu Tao , Yang Danyang , Li Xiaoli 
Published: June 2023

Background:
Circular RNA, as a member of noncoding RNA, plays an important role in the occurrence,
development and metastasis of tumor cells. So far, the correlation between circular RNA and malignant melanoma
remains obscure.

Methods:
RNA expression of circFAT1 and miR-375 in malignant melanoma (MM) tissues and cell lines was detected
by RT-PCR. The proliferation, cloning, migration and invasion of SK-Mel-28 and A375 cells were assessed using
CCK-8 test, clone formation and Transwell assay, respectively. CircRNA immunoprecipitation was used to validate the
relationship between circFAT1 and miR-375. The binding between circFAT1 and miR-375, as well as SLC7A11 and
miR-375 were verified by luciferase assay.

Results:
In our study，the circFAT1 was significantly overexpressed in the MM tissue than melanocytic nevi. Conversely,
the expression of miR-375 in MM tissue was lower than in melanocytic nevi tissue. The underexpression
of circFAT1 with siRNA plasmids significantly suppressed the proliferation, invasion and clone formation of MM cell
line. Mechanistically, circFAT1 positively regulates the expression level of SLC7A11 by sponging miR-375. The
promotive effects of circFAT1 on the proliferation and invasion ability of MM cells were reversed by the upregulation
of miR-375.

Conclusion:
circFAT1 promotes the proliferation, invasion and clone formation of malignant melanoma cells by improving
the expression level of SLC7A11 via sponging miR-375.