bluuebunny/crossref_metadata_2025_split huggingface dataset:
Linker histone H1 modulates defense priming and immunity in plants
Authors: Sheikh Arsheed H , Nawaz Kashif , Tabassum Naheed , Almeida-Trapp Marilia , Mariappan Kiruthiga G , Alhoraibi Hanna , Rayapuram Naganand , Aranda Manuel , Groth Martin , Hirt Heribert 
Published: February 2023

Abstract
Linker H1 histones play an important role in animal and human pathogenesis, but their function in plant immunity is poorly understood. Here, we analyzed mutants of the three canonical variants of Arabidopsis H1 histones, namely H1.1, H1.2 and H1.3. We observed that double h1.1h1.2 and triple h1.1h1.2h1.3 (3h1) mutants were resistant to Pseudomonas syringae and Botrytis cinerea infections. Transcriptome analysis of 3h1 mutant plants showed H1s play a key role in regulating the expression of early and late defense genes upon pathogen challenge. Moreover, 3h1 mutant plants showed enhanced production of reactive oxygen species and activation of mitogen activated protein kinases upon pathogen-associated molecular pattern (PAMP) treatment. However, 3h1 mutant plants were insensitive to priming with flg22, a well-known bacterial PAMP which induces enhanced resistance in WT plants. The defective defense response in 3h1 upon priming was correlated with altered DNA methylation and reduced global H3K56ac levels. Our data place H1 as a molecular gatekeeper in governing dynamic changes in the chromatin landscape of defense genes during plant pathogen interaction.
A Women in Medicine Trainees’ Council: a model for women trainee professional development
Authors: Warner A Sofia C , Ufere Nneka N , Patel Naomi J , Lau Emily S , Uchida Amiko M , Hills-Dunlap Kelsey , Bromberg Gabrielle K , Cunningham Elizabeth A , Tagerman Michelle D , Mills Gabrielle G , Palamara Kerri , Rigotti Nancy A , Burnett-Bowie Sherri-Ann M , Yeh Doreen DeFaria , Tanguturi Varsha K 
Published: February 2023

AbstractWomen physicians are promoted less often, more likely to experience harassment and bias, and paid less than their male peers. Although many institutions have developed initiatives to help women physicians overcome these professional hurdles, few are specifically geared toward physicians-in-training. The Women in Medicine Trainees’ Council (WIMTC) was created in 2015 to support the professional advancement of women physicians-in-training in the Massachusetts General Hospital Department of Medicine (MGH-DOM). In a 2021 survey, the majority of respondents agreed that the WIMTC ameliorated the challenges of being a woman physician-in-training and contributed positively to overall wellness. Nearly all agreed that they would advise other training programs to implement a similar program. We present our model for women-trainee support to further the collective advancement of women physicians.
RADON CONCENTRATION MEASUREMENT AND DOSE ESTIMATION IN NON-URANIUM MINES IN CHINA (2019–21)
Authors: Yanchao Song , Qingzhao Zhang , Hongxing Cui , Changsong Hou , Pinhua Zhang , Yunyun Wu 
Published: February 2023

Abstract
The radon concentration in selected non-uranium mines from 2019 to 2021 in China are presented. By using of solid-state nuclear track detector, a total of 110 non-uranium mines in 13 provinces and autonomous regions were monitored, and the data were analyzed according to the mineral categories. The arithmetic mean of radon concentration in underground metal mines and nonmetallic mines were 1180 and 162 Bq m−3, respectively, and the standard deviations were 2850 and 151 Bq m−3, respectively. Correspondingly, the average annual effective dose of metal miners was 12.7 mSv and that of nonmetal miners was 1.75 mSv. It was noted that the radon concentration in some metal mines is not optimal in China. It is suggested that the ventilation should be strengthened in some metal mines. Besides, it is still necessary to control occupational radon exposure of non-uranium miners as low as reasonably achievable, and non-uranium mines should be managed by classification.
Social Deafferentation and the Relation Between Loneliness and Hallucinations
Authors: Brederoo Sanne G , de Boer Janna N , Linszen Mascha M J , Blom Renske E , Begemann Marieke J H , Sommer Iris E C 
Published: February 2023

AbstractBackground and HypothesisThe social deafferentation hypothesis (SDA) has been proposed as an explanatory mechanism of hallucinations, based on the theory that social withdrawal triggers the initial phase of schizophrenia. The current study tests the SDA by assessing how loneliness is associated with different types of hallucinations. Under the SDA, increased loneliness is hypothesized to affect the occurrence of hallucinations that carry social meaning, but not of nonsocial hallucinations.Study DesignAs part of an online survey, 2038 adolescents and young adults from the general population (median age 21 years; 75% female) filled out the Questionnaire for Psychotic Experiences, and the shortened De Jong Gierveld Loneliness Scale. Binomial logistic regression was used to investigate the effects of loneliness severity on past month prevalence of hallucinations, and on the presence of social versus nonsocial hallucinations.Study ResultsLoneliness increased the prevalence of hallucinations across modalities in the past month. Moreover, stronger degree of loneliness increased the likelihood of hearing voices or laughter, and of hallucinating being touched. Conversely, loneliness decreased the likelihood of experiencing the nonsocial hallucination of a tingling feeling. As expected, loneliness did not increase the prevalence of experiencing nonsocial hallucinations. Surprisingly, neither was loneliness associated with experiencing felt presence.ConclusionsOur results are novel in showing that loneliness specifically increases the likelihood of hearing human sounds such as voices or laughter, or feeling a human touch. Hallucinations without social meaning were not more likely to be experienced with increasing loneliness. This forms a confirmation of the SDA.
Paracingulate Sulcus Length and Cortical Thickness in Schizophrenia Patients With and Without a Lifetime History of Auditory Hallucinations
Authors: Ćurčić-Blake Branislava , de Vries Annemarie , Renken Remco J , Marsman Jan Bernard C , Garrison Jane , Hugdahl Kenneth , Aleman André 
Published: February 2023

AbstractBackgroundIt has been theorized that hallucinations, a common symptom of schizophrenia, are caused by failures in reality monitoring. The paracingulate sulcus (PCS) has been implicated as a brain structure supporting reality monitoring with the absence or shorter length of PCS associated with an occurrence of hallucinations in schizophrenia. The absence or shorter length of PCS has been associated with an occurrence of hallucinations. There are inconsistent findings in the literature regarding the role of the asymmetry of this structure for hallucinations. Here, we investigated the length of the PCS and cortical thickness of surrounding structures in patients with a lifetime history of auditory verbal hallucinations (AVH).DesignSeventy-seven patients and twenty-eight healthy controls (HC) underwent an anatomical MRI scan. PCS length and cortical thickness were estimated using Mango brain visualization and FreeSurfer, respectively. Patients with AVH (n = 45) and patients without AVH were compared (n = 32) to the controls.ResultsPCS length significantly differed between HC and patient groups (F(2,102) = 3.57, P = .032) in the left but not in the right sulcus. We found significantly longer PCS between HC and AVH group but no difference between patient groups. Similarly, we found significant thinning of cortical structures including structures surrounding anterior parts of PCS between HC and patients either in general or per group, but no significant differences were observed between patient groups.ConclusionsPCS length in the left hemisphere is shorter in schizophrenia patients with hallucinations as compared to HC subjects. The patient group without hallucinations was in between those 2 groups. Cortical thickness of neighboring areas of PCS is diminished in patient groups relative to the healthy comparison subjects. The role of lateralization and functional involvement of the PCS region in processes underlying hallucinations, such as reality monitoring, needs further clarification.
Recreational Drug Use and Distress From Hallucinations in the General Dutch Population
Authors: van der Weijden-Germann Monique , Brederoo Sanne G , Linszen Mascha M J , Sommer Iris E C 
Published: February 2023

AbstractBackground and HypothesisDistress associated with auditory (AH) and visual (VH) hallucinations in the general population was found to be predictive of later need for mental healthcare. It is, therefore, important to understand factors relating to the distress individuals experience from their hallucinations. Hallucinations can easily occur under substance-induced states, but recreational drug use is also known as a self-medication strategy. The current study, therefore, investigated whether recreational drug use by individuals from the general population is associated with the degree of distress experienced from AH and/or VH.Study DesignDrug use and distress severity associated with AH (N = 3.041) and/or VH (N = 2.218) were assessed by means of an online survey in the general Dutch population (&gt;14 years of age).Study ResultsMultiple linear regression revealed that while past month consumption of alcohol was associated with less AH- and VH-related distress, past month cannabis use was associated with more AH- and VH-related distress. Furthermore, past month use of nitrous oxide was associated with more severe VH-related distress.ConclusionRecreational use of alcohol, cannabis, and nitrous oxide may play important differential roles in the degree of distress associated with AH and VH in individuals from the general population. The consumption of these substances could form a potential risk factor for the development of distressing hallucinations or function as a signal marker for their occurrence. Due to the cross-sectional design of the current study, the causal relation between recreational drug use and distressing hallucinations remains to be elucidated.
How Long Does Madness Take? Time and the Construction of Mental Illness in Community Mental Health Work
Authors: Whooley Owen 
Published: February 2023

ABSTRACT
How do temporal understandings shape the construction of social problems and the work of addressing them? This article takes up a social problem with an explicit time dimension – severe and persistent mental illness (SPMI) – to advocate for a focus on time in the analysis of social problems. Drawing on interviews with community mental health workers (n=100) and observations of a police department and a psychiatric emergency department, I show how the work of these organizations yields two distinct sociotemporal orders – what I deem pursuing quick closure for police and rushing to wait for emergency hospital staff. The sociotemporal orders influence how these workers construct and approach the problem of SPMI. While these sociotemporal orders enable coordination within organizations, they produce temporal incongruence across organizations, which leads to conflict and undermines wider system coordination. Because police and hospital staff are not on the same time, their organizations are not on the same page. This article underscores the extent to which local constructions of social problems carry temporal assumptions that have practical effects on the work of ameliorating such problems. I conclude by discussing how this temporal analysis provides insight into the operation of power in community mental health.
Postnatal administration of S-adenosylmethionine restores developmental AHR activation-induced deficits in CD8+ T-cell function during influenza A virus infection
Authors: Post Christina M , Myers Jason R , Winans Bethany , Lawrence B Paige 
Published: February 2023

AbstractDevelopmental exposures can influence life-long health; yet, counteracting negative consequences is challenging due to poor understanding of cellular mechanisms. The aryl hydrocarbon receptor (AHR) binds many small molecules, including numerous pollutants. Developmental exposure to the signature environmental AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) significantly dampens adaptive immune responses to influenza A virus in adult offspring. CD8+ cytotoxic T lymphocytes (CTL) are crucial for successful infection resolution, which depends on the number generated and the complexity of their functionality. Prior studies showed developmental AHR activation significantly reduced the number of virus-specific CD8+ T cells, but impact on their functions is less clear. Other studies showed developmental exposure was associated with differences in DNA methylation in CD8+ T cells. Yet, empirical evidence that differences in DNA methylation are causally related to altered CD8+ T-cell function is lacking. The 2 objectives were to ascertain whether developmental AHR activation affects CTL function, and whether differences in methylation contribute to reduced CD8+ T-cell responses to infection. Developmental AHR triggering significantly reduced CTL polyfunctionality, and modified the transcriptional program of CD8+ T cells. S-adenosylmethionine, which increases DNA methylation, but not Zebularine, which diminishes DNA methylation, restored polyfunctionality and boosted the number of virus-specific CD8+ T cells. These findings suggest that diminished methylation, initiated by developmental exposure to an AHR-binding chemical, contributes to durable changes in antiviral CD8+ CTL functions later in life. Thus, deleterious consequence of development exposure to environmental chemicals is not permanently fixed, opening the door for interventional strategies to improve health.
Biosimilars for Pediatric Patients With Inflammatory Bowel Disease: Pediatric Gastroenterology Clinical Practice Survey
Authors: Maltz Ross M. , McClinchie Madeline G. , Boyle Brendan M. , McNicol Megan , Morris Grant A. , Crawford Erin C. , Moses Jonathan , Kim Sandra C. 
Published: February 2023

Background:
Biosimilars are biological agents that have been demonstrated to have similar safety and efficacy profiles as the originator. The objective of this study was to evaluate the perspectives of pediatric gastroenterologists in the United States (U.S.) toward biosimilar use and to explore factors that impact their comfort level with prescribing infliximab biosimilars.

Methods:
A cross-sectional survey was developed and distributed to pediatric gastroenterology physicians from the U.S. via a listserv (Pediatric gastroenterology Bulletin Board). Respondent’s demographics were recorded. Using a 6-point Likert scale, the survey assessed the respondent’s perceptions toward biosimilars and initiating switches from the originator to biosimilar agent along with factors impacting provider’s comfort level. Fischer exact tests were used to detect statistically significant differences in responses for hypotheses of interest.

Results:
One hundred thirty-nine pediatric gastroenterologists completed the online survey (response rate 5.4%). Eighty-seven percent of respondents reported being comfortable prescribing infliximab biosimilars to anti-tumor necrosis factor naive patients, and 69% reported being comfortable doing a one-time switch if the patient was in clinical remission. Factors that negatively impacted a respondent’s comfort level included respondents not practicing at an ImproveCareNow (ICN) center and managing less than 50 patients with inflammatory bowel diseases (IBD).

Conclusions:
Nearly 90% of pediatric gastroenterologists felt comfortable prescribing an infliximab biosimilar, and 70% felt comfortable with a one-time switch to the biosimilar if the patient was in clinical remission. Involvement in ICN a learning health system and caring for higher numbers of patients with IBD was associated with increased provider comfort with biosimilar use.
Prevalence and Risk Factors of Urinary Retention in Patients With Cervical Cancer
Authors: Bai Zhilan , Zuo Yan , Huang Wenxia , Yao Li 
Published: February 2023

Background
The literature is inconsistent on the prevalence and risk factors of urinary retention in patients with cervical cancer.

Objective
The aim of this study was to review the literature on the prevalence of urinary retention in patients with cervical cancer and consolidate the risk factors.

Methods
For this meta-analysis, eligible articles published in English or Chinese by December 10, 2021, were systematically searched for and retrieved from PubMed, Cochrane Library, Ovid-Embase Medline, Web of Science, PsycINFO, CINAHL, and Scopus. Prevalence, odds ratios (ORs), and 95% confidence intervals (CIs) were used for meta-analysis.

Results
Twenty-five studies were included in the analysis. The pooled overall prevalence was 0.26 (95% CI, 0.21-0.30, I
2 = 95.0%). The identified risk factors were age (OR, 1.13; 95% CI, 1.08-1.19), urinary tract infection (UTI) (OR, 3.33; 95% CI, 1.48-7.49), surgical extent (OR, 2.95; 95% CI, 1.27-6.85), and catheter indwelling time (OR, 3.44; 95% CI, 2.43-3.87).

Conclusions
The prevalence of urinary retention in patients with cervical cancer is 0.26. Older age, UTI, longer catheter indwelling time, and a larger surgical extent may increase the risk of urinary retention. Clinicians should identify patients at risk and adopt interventions such as individualized catheter care.

Implications for Practice
Nursing staff should assess the risk of urinary retention in a patient with cervical cancer according to her age, presence of UTI, surgical extent, and catheterization time. A carefully chosen surgical procedure and interventions such as individualized education, timely catheter removal, treatment of UTI, and rehabilitation should be offered.
Cooperativity between H3.3K27M and PDGFRA poses multiple therapeutic vulnerabilities in human iPSC-derived diffuse midline glioma avatars
Authors: Skinner Kasey R. , Koga Tomoyuki , Miki Shunichiro , Gruener Robert F. , Grigore Florina-Nicoleta , Torii Emma H. , Seelig Davis M. , Suzuki Yuta , Kawauchi Daisuke , Lin Benjamin , Malicki Denise M. , Chen Clark C. , Benveniste Etty N. , Patel Rakesh P. , McFarland Braden C. , Huang R. Stephanie , Jones Chris , Mackay Alan , Miller C. Ryan , Furnari Frank B. 
Published: February 2023

AbstractDiffuse midline glioma (DMG) is a leading cause of brain tumor death in children. In addition to hallmark H3.3K27M mutations, significant subsets also harbor alterations of other genes, such asTP53andPDGFRA. Despite the prevalence of H3.3K27M, the results of clinical trials in DMG have been mixed, possibly due to the lack of models recapitulating its genetic heterogeneity. To address this gap, we developed human iPSC-derived tumor models harboring TP53R248Qwith or without heterozygous H3.3K27M and/or PDGFRAD842Voverexpression. The combination of H3.3K27M and PDGFRAD842Vresulted in more proliferative tumors when gene-edited neural progenitor (NP) cells were implanted into mouse brains compared to NP with either mutation alone. Transcriptomic comparison of tumors and their NP cells of origin identified conserved JAK/STAT pathway activation across genotypes as characteristic of malignant transformation. Conversely, integrated genome-wide epigenomic and transcriptomic analyses, as well as rational pharmacologic inhibition, revealed targetable vulnerabilities unique to the TP53R248Q; H3.3K27M; PDGFRAD842Vtumors and related to their aggressive growth phenotype. These includeAREG-mediated cell cycle control, altered metabolism, and vulnerability to combination ONC201/trametinib treatment. Taken together, these data suggest that cooperation between H3.3K27M and PDGFRA influences tumor biology, underscoring the need for better molecular stratification in DMG clinical trials.
Pro-inflammatory polarization and colorectal cancer modulate alternative and intronic polyadenylation in primary human macrophages
Authors: Wilton Joana , de Mendonça Filipa Lopes , Pereira-Castro Isabel , Tellier Michael , Nojima Takayuki , Costa Angela M , Freitas Jaime , Murphy Shona , Oliveira Maria Jose , Proudfoot Nicholas J , Moreira Alexandra 
Published: February 2023

SummaryMacrophages are essential cells of the immune system that alter their inflammatory profile depending on their microenvironment. Alternative polyadenylation in the 3’UTR (3’UTR-APA) and intronic polyadenylation (IPA) are mechanisms that modulate gene expression, in particular in cancer and activated immune cells. Yet, how polarization and colorectal cancer (CRC) cells microenvironment affect 3’UTR-APA and IPA in primary human macrophages remains unknown. Here, primary human monocytes were isolated from healthy donors, differentiated and polarized into a pro-inflammatory state and ChrRNA-Seq and 3’RNA-Seq were performed to quantify gene expression and characterize new 3’UTR-APA and IPA mRNA isoforms. Our results show that polarization of human macrophages from naïve to a pro-inflammatory state causes a marked increase both in proximal polyA site selection in the 3’UTR and in IPA events, in genes relevant for macrophage functions. Additionally, we found a negative correlation between differential gene expression and IPA during pro-inflammatory polarization of primary human macrophages. As macrophages are abundant immune cells in the CRC microenvironment that either promote or abrogate cancer progression, we investigated how indirect exposure to CRC cells affects macrophage gene expression and 3’UTR-APA and IPA mRNA events. Co-culture with CRC cells alters the inflammatory phenotype of macrophages, increases the expression of pro-tumoral genes and induce 3’UTR-APA alterations. Notably, some of these gene expression differences were also found in tumour-associated macrophages of CRC patients, indicating that they are physiological relevant. Upon macrophage pro-inflammatory polarizationSRSF12is the pre-mRNA processing gene that is most upregulated. AfterSRSF12knockdown in M1 macrophages there is a global downregulation of gene expression, in particular in genes involved in gene expression regulation and in immune responses. Our results reveal new 3’UTR-APA and IPA mRNA isoforms produced during pro-inflammatory polarization of primary human macrophages and CRC co-culture that may be used in the future as diagnostic or therapeutic tools.