bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Diagnostic Performance, Semiquantitative Analysis, and Staging Performance of [18F]FAPI-42 and 2-[18F]FDG PET/CT in Lymph Node Metastases
Authors: Mu Xingyu , Huang Jinquan , Li Meng , Jiang Zewen , Jia Lulu , Zhao Haibo , Lv Zhongyuan , Fu Wei 
Published: June 2023

Abstract
Background
This study was to compare the diagnostic performance, semiquantitative analysis, and staging performance of [18F]-labelled fibroblast activation protein inhibitor ([18F]FAPI-42) and 2-[18F]fluoro-2-deoxy-D-glucose(2-[18F]FDG) PET/CT in lymph node (LN) metastases.
Methods
From the detection of metastatic LNs, the semiquantitative value of the LN metastases, and the evaluation of N staging, a retrospective analysis of 56 patients diagnosed with LNs metastases who underwent [18F]FAPI-42 and 2-[18F]FDG PET/CT scans within a week for staging or restaging was performed. We analyzed, and compared the diagnostic performance and SUVmax of primary tumors and recurrent lesions, as well as the SUVmax, TBR, and diagnostic performance of metastatic LNs between [18F]FAPI-42 and 2-[18F]FDG PET/CT.
Results
A total of 216 metastatic LNs from 56 patients were detected and semi-quantitatively analyzed. Compared to 2-[18F]FDG PET/CT, [18F]FAPI-42 PET/CT presented a better diagnostic performance based on patients (98.2% vs. 92.9%, P = 0.364), based on lesions (87.5% vs. 78.7%, P = 0.015). Regarding semiquantitative analysis, the SUVmax of LNs metastases on [18F]FAPI-42 PET/CT presented higher uptake than that on 2-[18F]FDG PET/CT (SUVmax 5.0 vs. 3.9, P = 0.002). For differentiating metastatic LNs to normal LNs, the SUVmax of [18F]FAPI-42 in metastatic LNs was significantly higher in the non-metastatic LNs (SUVmax, 5.0 vs. 1.2, P < 0.001). Comparing the management of N staging between two tracers, [18F]FAPI-42 estimated precisely more patients than 2-[18F]FDG PET/CT (83.9% vs. 78.4%, P = 0.468).
Conclusion
[18F]FAPI-42 PET/CT showed superior diagnostic performance, the quantitative capability of metastatic LNs, and the management of N staging in patients with cancers compared to 2-[18F]FDG PET/CT.
Global Research Status and Trends of Tibial plateau fracture Over the Past 28 Years: A Historical Review and Bibliometric Analysis
Authors: Yan Hongsong , Yu Yifan , Hu Minhua , Luo Weidong , Huang Feng , Ge Hao 
Published: June 2023

Abstract
Background:Tibial plateau fracture (TPF) is a complex intraarticular fracture that has been extensively researched. However, there has been a lack of bibliometric studies investigating global research trends in TPF. This study aims to analyze the knowledge framework, research hotspots, and thematic trends in the field of TPF research.
Methods: Scientific outputs related to TPF from 1994 to 2022 were retrieved from the Web of Science Core Collection. Three bibliometric tools, namely CiteSpace, VOSviewer, and an online analytical platform, were employed for the analysis. The main analyses encompassed contributions from countries, publication and citation counts, institutions, authors, funding agencies, and journals, as well as keyword clustering.
Results: A total of 884 articles were identified, and the number of annual publications on TPF exhibited a generally upward trend. China emerged as the most prominent contributor with the highest number of publications, while the United States had the largest H-index. Hebei Medical University made the most significant investment in this field. Professors Zhang Yingze, Luo Congfeng, Egol Kenneth A, and Chen Wei emerged as core authors in this field. The most productive journal was Injury-International Journal of the Care of the Injured. The keywords were clustered into six groups: Epidemiology and prognosis, Internal fixation and complications, Surgical protocol and finite element analysis, Diagnosis and new techniques, Complex fracture types, and Bone graft. These clusters showed a trend of balanced and diversified development. The ongoing outbreak keywords included "debris," "classification," "trauma," "posterolateral fracture," and "risk factors," which were considered as future research hotspots deserving more attention.
Conclusions:Surgical protocols and bone defect filling materials for complex types of TPF have received increasing attention from orthopedic surgeons. It is anticipated that these research topics will continue to be significant and hold a prominent position in the near future.
Cyclin D1·Cdk4 regulates neuronal activity through phosphorylation of GABAA receptors
Authors: Pedraza Neus , Monserrat Maria Ventura , Ferrezuelo Francisco , Torres-Rosell Jordi , Colomina Neus , Miguez-Cabello Federico , Picañol-Párraga Javier , Soto David , López-Merino Esperanza , García-Vilela Celia , Esteban Jose Antonio , Egea Joaquim , GARI ELOI 
Published: June 2023

Abstract
Nuclear Cyclin D1 (Ccnd1) is a main regulator of cell cycle progression and cell proliferation. Interestingly, Ccnd1 moves to the cytoplasm at the onset of differentiation in neuronal precursors. However, the cytoplasmic functions and targets of Ccnd1 in post-mitotic neurons are unknown. Here we identify the α4 subunit of gamma-aminobutyric acid (GABA) type A receptors (GABAARs) as an interactor and target of Ccnd1·Cdk4. Ccnd1 binds to an intracellular loop in α4 and, together with Cdk4, phosphorylates the α4 subunit at threonine 423 and serine 431. These modifications upregulate α4 surface levels and increase the activity of α4-containing GABAARs, measured in whole-cell patch-clamp recordings. In agreement with this role of Ccnd1·Cdk4 in neuronal signaling, inhibition of Cdk4 or expression of the non-phosphorylatable α4 decrease synaptic and extrasynaptic currents in the hippocampus of newborn rats. Moreover, according to α4 functions in synaptic pruning, CCND1 knockout mice display an altered pattern of dendritic spines that is rescued by the phosphomimetic α4. Overall, our findings molecularly link Ccnd1·Cdk4 to GABAARs activity in the central nervous system and highlight a novel role for this G1 cyclin in neuronal signaling.
Exploring single nucleotide polymorphisms in the KCNQ1 gene associated with cardiac disorders
Authors: Rida Mariam , Shahzadi Tabinda , Nathaniel Sammar 
Published: June 2023

Abstract
Voltage gated potassium ion channels play a crucial role in the proper functioning of excitable cells, the appropriate secretion of hormones and neurotransmitters and especially in the maintenance of cardiac function. Among these channels, KCNQ1 channel has significant importance in modulating the action potential, in facilitating dynamic cellular repolarization of cardiac tissues and in regulating the water and salt balance in various tissues throughout the human body. The differential activity of KCNQ1 channels in these tissues is facilitated by accessory proteins and modulating factors that modify the properties of the KCNQ1 channel. KCNQ1 is different from other potassium ion channels as it has the ability to act as either a voltage-dependent channel that requires membrane depolarization for its activation or a fundamentally active channel. Mutations including indels and single nucleotide polymorphisms in the KCNQ1 gene result in dysfunction of the channel causing a number of different disorders and syndromes including short and long QT disorders, Jervell-Lange Nielsen Syndrome, Romano-Ward Syndrome and familial atrial fibrillation. This article reviews the unique structural and functional properties, the diverse physiological roles of KCNQ1 channel with a major focus on the mutational spectrum of the KCNQ1 gene, its implications and the therapeutic approaches being employed to manage the resultant disorders.
‘Social’ versus ‘Asocial’ cells--- Dynamic Competition Flux Balance Analysis
Authors: Liu Yanhua , Westerhoff Hans 
Published: June 2023

Abstract
In multicellular organisms, different cell types compete for resources or growth factors, endangering cellular diversity as well as co-existence. To address this, we developed ‘dynamic cell-cell competition FBA’ (dcFBA). With total biomass synthesis as objective, we found that lower-growth-yield cell types face extinction even when they synthesized mutually required metabolic commodities. Signal transduction between cells promoted co-existence, when turning the cells into mutually regulatory and responsive ‘social cells’. Mutants with specific growth rate but intact signal transduction did not outgrow others. However, loss of its social characteristics enabled a mutant to dominate the other cell types with higher specific growth rates and bring those to extinction. A corollary is that cancer arises from reduced sensitivity to regulatory factors rather than enhanced specific growth rates. Therapies reinforcing cells’ cross-regulation, perhaps through alternative signaling routes, may therefore be more effective than those targeting replication rates.
First Case of Female Prune Belly Syndrome Following In Vitro Fertilization-Induced Pregnancy: A Rare Case Report and Systematic Review of the Literature
Authors: Alkhawaldeh Ibraheem M. , Jaradat Jaber Husam , Al-Jafari Mohammad , Nashwan Abdulqadir J. , Alrahamneh Samer Irshaid 
Published: June 2023

Abstract
Prune belly syndrome (PBS) is a rare congenital anomaly characterized by a triad of abdominal flaccidity, varying degrees of urinary system involvement, and cryptorchidism. The exact cause of PBS is unknown. Clinical symptoms can range from stillbirth to significant renal and respiratory abnormalities to almost normal children. Treatment typically involves surgical repair of the abdominal wall defect and urinary tract abnormalities, early orchiopexy, and supportive management of related problems. We report the first case of a female newborn with PBS following in vitro fertilization-induced pregnancy with a comprehensive systematic review of all relevant cases.
Performance Evaluation of Domain Isolation Multi-controller in Software Defined Networking Architecture on Mininet
Authors: Prakoso Dino Budi , Salman Muhammad , Sari Riri Fitri , Djemame Karim 
Published: June 2023

Abstract
The growing digitalization of the automotive, transportation, urban mobility, and telecommunications industries highlights the significance of point-to-multipoint communication services. These services are essential for maximizing the efficiency of hardware and software resources and ensuring that users consistently receive software updates and communications. In order to implement precise and dependable point-to-multipoint distribution services within the network, software-defined networking (SDN) is used. SDN controllers like RYU, Floodlight, and others have become the de facto standard for operating these kinds of networks. An SDN multi-controller's efficiency greatly affects the underlying SDN infrastructure network's adaptability and capabilities. This research applied of Mininet emulator to develop a multi-controller SDN architecture with a Fat-Tree topology and two RYU controllers. Throughput, latency, and round-trip time are some of the node-to-node performance metrics that will be used to evaluate the effectiveness of a Domain Isolation Multi-controller network architecture.
The Single and Interactive Effects of Aluminium and Low Ph, or Ca/al Ratios on Growth of Red Pine Seedlings
Authors: SHAN YUNFENG 
Published: June 2023

Abstract
Acid deposition has been one of the major environmental pollution problems for longer than 50 years, since the 1970s. Investigation on the interaction of aluminum (Al) and low pH effects on red pine (Pinus densiflora Sieb. and Zucc.) has been required but lacking. In the present study, needles of red pine seedlings exposed to Al treatments with solution pH 4.0 and 3.5 exhibited purplish leaf characteristics of Al toxicity. The dry weights of the needle and whole plant, and the current needle elongation were linearly reduced with Al concentrations from 0, 13 to 26 ppm. Results show that red pine is an intermediate species in sensitivity to Al and is insensitive to low pH. However, the synergistic interactions of low pH treatments with the elevated Al were significant. Al toxicity to the root elongation, needle matter production, and needle elongation of red pine was significantly enlarged with reduced pH. The root length and whole plant length were significantly decreased at 1:10 of Ca/Al ratios (p < 0.05). Al phytotoxicity was completely lost when the Ca/Al molar ratio was 10:1. Liming is still an applicable measure to remediate acidification problems by natural or anthropogenic factors such as acid deposition.
Investigation of the Effects of Mebendazole on the Caspase-mediated Apoptosis Mechanism in Cancer Cell Culture
Authors: ŞAHİN MAHMUT , KARA Haki 
Published: June 2023

Abstract
This study was conducted to compare mebendazole in terms of its apoptosis-inducing and tubulin-inhibitory effects when combined with vincristine and paclitaxel, both of which are used in cancer treatment Lung fibroblast cells (MRC-5) and small cell lung carcinoma (NCI-H209) cell lines were used in the study. Concentrations of mebendazole, vincristine, and paclitaxel at 0.5 µM, 1 µM, 1.5 µM, and 2 µM were separately applied to these cell lines, as well as in combinations. After the cells were kept in the culture medium for 24 hours following drug administration, cell proliferation, apoptotic DNA levels, caspase 3, 8, and 9 levels, and in-vitro wound healing experiments were performed. It was determined that mebendazole suppressed cell proliferation and cell healing, increased caspase-3, caspase-8, caspase-9 levels and apoptotic DNA formation in NCI-H209 cancer lung cells. Compared to the groups given mebendazole and vincristine alone, it was observed that cell proliferation was more suppressed and, the level of apoptosis increased in cancerous cells in the groups given the combination of the two drugs. According to the findings obtained from our study, we believe that mebendazole may possess therapeutic activity against cancerous lung cells (NCI-H209) due to its apoptosis-inducing and cell proliferation-suppressive effects.
GGH is a potential prognostic and immunotherapeutic biomarker in gliomas and promotes the proliferation, migration, and invasion of human glioma cells
Authors: Xu Yang , Yan Zheng , Hou Chengshan , Teng Hong , Shi Jianwei , Fu Rui , Zhang Yuhai 
Published: June 2023

Abstract
Gramma-glutamyl-hydrolase (GGH) has been implicated in antitumor resistance and unfavourable prognosis across various tumour types. However, the potential role and mechanism of GGH in gliomas remain poorly understood. We undertook the bioinformatics analysis to systematically explore the correlation between GGH expression and prognosis, clinical features, immune cell infiltration, tumor microenvironment and biological functions in the TGGA database. Subsequently, we performed knockdown and overexpression experiments on two gliomas cell lines (U251 and A299) and verified their expression stability using Western blotting assay. Cell function assays, including EdU immunofluorescence, colony formation, transwell assay and wound-healing assay, were conducted to investigate the impact of GGH expression on proliferation, invasion and migration of glioma cells in vitro. Additionally, a subcutaneous xenograft experiment was performed to evaluate the effect of GGH expression on tumor growth in vivo. GGH was highly expressed in gliomas and correlated with poor prognosis, clinic features and the microenvironment of gliomas. Cell function assays manifested that overexpression of GGH promoted the proliferation, migration, and invasion of glioma cells, while knockdown of GGH had the opposite effect. In vivo xenograft experiments showed that GGH knockdown suppressed the growth rate of gliomas. We identified GGH as a relevant biomarker for the malignant behaviors of gliomas and it holds great promise in therapeutic strategy and prognostic evaluation.
Effectof application cycle of beta-cypermethrin nano-emulsion and common emulsifiable concentrate on Comparative study on the activity of resistance-related enzymes of Blattella Germany
Authors: Shen Yan , Li Qiong , Fang Fujin , Yang Chuanli , Yu Dong , Shen Xiaobing 
Published: June 2023

Abstract
Objective: To investigate the effects of different application cycles of beta-cypermethrin nano-emulsion and beta-cypermethrin common emulsion on the activities of resistance-related enzymes in Blattella Germany.
Methods: Blattella Germany was treated with micro-drop method, and the enzyme activity of Blattella germanica was measured at different time after 18 days. The enzyme activities of Blattella Germany were determined by spectrophotometry, and the relationship between enzyme activities and application cycle of beta-cypermethrin nano-emulsion and beta-cypermethrin common emulsion was studied by contour analysis.
Results: The profiles of acetylcholinesterase (AchE), glutathione S-transferase (GST) and P450-O demethylase activities of Blattella Germany exposed to beta-cypermethrin nanoemulsion and beta-cypermethrin conventional emulsion were not parallel (F values were 174.025,1 017.969 and 1 364.271, respectively, P<0.001).
Conclusion: The effects of beta-cypermethrin nano-emulsion and beta-cypermethrin common emulsion on the activities of AchE, GST and P450-O demethylase in Blattella Germany were different, and the profiles were neither parallel nor overlapped.
Communications：87 dingjiaqiao, gulou district, nanjing city, jiangsu province, China
Latent Drug Recognition Using Fuzzy Matching in Sequential Analysis of Full-Text Health Related Publications: Case of a Health Dissemination tool in a developing country
Authors: Mulunda Christine K. , Wagacha Peter W. , Muchemi Lawrence 
Published: June 2023

Abstract
Health related discoveries are mainly published as journal publications and the rate at which they are generated increases as new information and discoveries emerge. Discovery of latent medically-related terms in a document corpus is a challenging task where the researcher is not an expert in that domain and a viable database of medicine related words is not readily available. The study focused on investigating methodologies and best practises that will enable discovery of latent drug terms found in health publications corpus for effective dissemination at county and national levels. Fuzzy matching methodology was considered for its near and exact matching algorithms. DrugBank dataset was chosen as reference for drug terms because of its comprehensive list of drugs, that are frequently updated and freely accessible. Semi-supervised learning was applied in modelling of multi-search medical terms on an hourly basis. drug name Recognition, Sentence Categorization and Information Retrieval are among the features described in the presented model.
Randomized-controlled trial comparing cosmetic results of midline incision versus off- midline incision for specimen-extraction in laparoscopic colectomy
Authors: Iwamoto Hiromitsu , Matsuda Kenji , Takifuji Katsunari , Tamura Koichi , Mitani Yasuyuki , Mizumoto Yuki , Nakamura Yuki , Sakanaka Toshihiro , Yokoyama Shozo , Hotta Tsukasa , Yamaue Hiroki 
Published: June 2023

Abstract
Purpose
A notable advantage of laparoscopic colorectal surgery is that only a small incision at the extraction site is necessary, which is considered to be cosmetically beneficial. Meanwhile, the optimal extraction site for the resected specimen in laparoscopic colectomy is controversial in terms of cosmetic benefit. This randomized controlled trial compares midline and off-midline extraction sites in laparoscopic colectomy in patients with colon cancer, with consideration of cosmetic benefits as the primary endpoint.
Methods
Included were patients that underwent elective laparoscopic colectomy at WMUH between October 2014 and February 2017. Patients were randomly assigned to either midline incision group or to off-midline incision group. Prospectively-collected data included cosmetic results (patients and observer assessment scale) and complications including incidence of incisional hernia, SSI and pain. This trial was registered with UMIN Clinical Trials (UMIN000028943).
Results
Finally, 98 patients with colorectal cancer were analyzed. No significant differences were found between the two groups in patient and observer assessment scales of cosmetic results (midline 8 ± 1.1 vs off-midline 11 ± 5.9 p = 0.16, midline 13.5 ± 6.6 vs off-midline 15 ± 11 p = 0.58, respectively) or in postoperative pain. However, incisional hernia occurred in four cases in the midline group (8%), which was significantly higher than that in the off-midline group (no cases, 0%).
Conclusion
There was no significant difference in terms of cosmetic benefit, the primary endpoint, between the two groups. In this study, only the extraction site location was compared; future studies will examine differences depending on the incisional direction, including the incidence of incisional hernia.
Alteration of HIF1a target genes led to 5FU resistance in gastric cancer MKN45 cells under hypoxic condition
Authors: Sharifi Hengameh , Khorashadizadeh Mohsen 
Published: June 2023

Abstract
Purpose

Gastric cancer (GC) is reported as the fifth most commonly diagnosed cancer and is the fourth most common cause of cancer-associated death in the world. Tumor cell hypoxia is one of the main factors causing 5FU resistance in gastric cancer cells. Herein, we intend to evaluate the efficacy of the 5FU on the MKN45 cell line by establishing an in vitro hypoxic environment and comparing them with results from the normoxic condition. This study aims to evaluate the efficacy of the 5FU on the MKN45 cell line in normoxic and hypoxic conditions.

Methods

The MKN45 gastric cancer cell line was cultured in normoxic and hypoxic conditions. The cells were treated with various concentrations of 5-FU for 72 h. Then, cell viability was analyzed by the MTT method. Also, the induction of apoptosis was analyzed by flow cytometry. The expression levels of HIF-1 a, P53, BAX, Bcl2, MRP1, and Casp3 genes were quantified by real-time PCR. A statistical analysis of the results was done using SPSS software.

Results

Our study showed that a hypoxic condition leads to a higher resistance against 5-FU toxicity in MKN45 cells compared to normoxia. As a result of this drug resistance, we also found significantly low apoptotic cells in hypoxic conditions. Data of gene expression in 5-FU treated MKN45 cells, indicated significant up-regulation of HIF1a in hypoxic conditions. We also showed an elevated level of pro-apoptotic genes (Bax and casp3) in the normoxic and hypoxic groups, but this elevation was significantly lower in hypoxia. In contrast, significant down-regulation of the anti-apoptotic gene (Bcl2) was detected just in the normoxic group, while the Bcl2 gene was significantly up-regulated in the hypoxia versus normoxia group. In the case of p53 and MRP1 genes, we found a higher level of gene expression in MKN45 cells treated under normoxic and hypoxic conditions compared to control, while this increase was more significant in hypoxic conditions. There was no significant difference in the level of expression of the MRP1 gene in hypoxic conditions compared to normoxia.

Conclusion

Altogether, our results demonstrated that the resistance to 5-FU in MKN45 gastric cancer cells might be due to the upregulation of the HIF-1α gene and its regulated downstream target gene under hypoxic conditions.