bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conversion of specific lncRNAs to biomarkers in exhaled breath condensate samples of patients with advanced stage non-small-cell lung cancer
Authors: Tetik Vardarlı Aslı , Ozgur Su , Goksel Tuncay , Korba Korcan , Karakus Hardar Soydaner , Asık Aycan , Pelit Levent , Gunduz Cumhur 
Published: June 2023

Objectives: Lung cancer (LC) is one of the most prevalent cancers with the highest fatality rate worldwide. Long noncoding RNAs (lncRNAs) are being considered potential new molecular targets for early diagnosis, follow-up, and individual treatment decisions in LC. Therefore, this study evaluated whether lncRNA expression levels obtained from exhaled breath condensate (EBC) samples play a role in the occurrence of metastasis in the diagnosis and follow-up of patients with advanced lung adenocarcinoma (LA).Methods: A total of 40 patients with advanced primary LA and 20 healthy controls participated in the study. EBC samples were collected from patients (during diagnosis and follow-up) and healthy individuals for molecular analysis. Liquid biopsy samples were also randomly obtained from 10 patients with LA and 10 healthy people. The expression of lncRNA genes, such as MALAT1, HOTAIR, PVT1, NEAT1, ANRIL, and SPRY4-IT1 was analyzed using cfRNA extracted from all clinical samples.Results: In the diagnosis and follow-up of patients with LA, lncRNA HOTAIR (5-fold), PVT1 (7.9-fold), and NEAT1 (12.8-fold), PVT1 (6.8-fold), MALAT1 (8.4-fold) expression levels were significantly higher than those in healthy controls, respectively. Additionally, the distinct lncRNA expression profiles identified in EBC samples imply that decreased ANRIL–NEAT1 and increased ANRIL gene expression levels can be used as biomarkers to predict the development of bone and lung metastases, respectively.Conclusion: EBC is an innovative, easily reproducible approach for predicting the development of metastases, molecular diagnosis, and follow-up of LC. EBC has shown potential in elucidating the molecular structure of LC, monitoring changes, and discovering novel biomarkers.
Influence of antipsychotic medications on hyperlipidemia risk in patients with schizophrenia: evidence from a population-based cohort study and in vitro hepatic lipid homeostasis gene expression
Authors: Wu Tien-Yuan , Tien Ni , Lin Cheng-Li , Cheah Yu-Cun , Hsu Chung Y. , Tsai Fuu-Jen , Fang Yi-Jen , Lim Yun-Ping 
Published: June 2023

IntroductionSchizophrenia increases the risk of mortality and cardiovascular disease (CVD) risk. However, the correlation between antipsychotics (APs) and CVD remains controversial. Hyperlipidemia is a significant risk factor for CVD.MethodsWe conducted a nationwide population-based retrospective cohort study to investigate the effects of APs on the risk of hyperlipidemia and lipid homeostasis gene expression. We used data from the Longitudinal Health Insurance Database of Taiwan on new-onset schizophrenia patients and a comparison cohort without schizophrenia. We used a Cox proportional hazards regression model to analyze the differences in hyperlipidemia development between the two cohorts. Furthermore, we examined the effects of APs on the hepatic expression of lipid homeostasis-related genes.ResultsAfter adjusting for potential interrelated confounding factors, the case group (N = 4,533) was found to have a higher hyperlipidemia risk than the control cohort (N = 4,533) [adjusted hazard ratio (aHR), 1.30, p &lt; 0.001]. Patients with schizophrenia without APs had a significantly higher risk of hyperlipidemia (aHR, 2.16; p &lt; 0.001). However, patients receiving APs had a significantly lower risk of hyperlipidemia than patients not receiving APs (all aHR ≤ 0.42, p &lt; 0.001). First-generation antipsychotics (FGAs) induce the expression of hepatic lipid catabolism genes in an in vitro model.DiscussionPatients with schizophrenia had a higher risk of hyperlipidemia than controls; however, compared with non-treated patients, AP users had a lower risk of hyperlipidemia. Early diagnosis and management of hyperlipidemia may help prevent CVD.
Altered brain connectivity in Long Covid during cognitive exertion: a pilot study
Authors: Barnden Leighton , Thapaliya Kiran , Eaton-Fitch Natalie , Barth Markus , Marshall-Gradisnik Sonya 
Published: June 2023

IntroductionDebilitating Long-Covid symptoms occur frequently after SARS-COVID-19 infection.MethodsFunctional MRI was acquired in 10 Long Covid (LCov) and 13 healthy controls (HC) with a 7 Tesla scanner during a cognitive (Stroop color-word) task. BOLD time series were computed for 7 salience and 4 default-mode network hubs, 2 hippocampus and 7 brainstem regions (ROIs). Connectivity was characterized by the correlation coefficient between each pair of ROI BOLD time series. We tested for HC versus LCov differences in connectivity between each pair of the 20 regions (ROI-to-ROI) and between each ROI and the rest of the brain (ROI-to-voxel). For LCov, we also performed regressions of ROI-to-ROI connectivity with clinical scores.ResultsTwo ROI-to-ROI connectivities differed between HC and LCov. Both involved the brainstem rostral medulla, one connection to the midbrain, another to a DM network hub. Both were stronger in LCov than HC. ROI-to-voxel analysis detected multiple other regions where LCov connectivity differed from HC located in all major lobes. Most, but not all connections, were weaker in LCov than HC. LCov, but not HC connectivity, was correlated with clinical scores for disability and autonomic function and involved brainstem ROI.DiscussionMultiple connectivity differences and clinical correlations involved brainstem ROIs. Stronger connectivity in LCov between the medulla and midbrain may reflect a compensatory response. This brainstem circuit regulates cortical arousal, autonomic function and the sleep–wake cycle. In contrast, this circuit exhibited weaker connectivity in ME/CFS. LCov connectivity regressions with disability and autonomic scores were consistent with altered brainstem connectivity in LCov.
Advancing radioactive material research method: the development of a novel in situ particle-attached microfluidic electrochemical cell
Authors: Yao Jennifer , Tripathi Shalini , McNamara Bruce K. , Lahiri Nabajit , Riechers Shawn L. , Chatterjee Sayandev , Reilly Dallas D. , Ilton Eugene S. , Buck Edgar C. 
Published: June 2023

Introduction: This study aims to develop a microgram-scale microfluidic electrochemical cell (E-cell) for investigating the redox behavior of uranium oxide (UO2). The traditional bulk electrochemical methods may require shielded facilities to investigate the hazardous materials, e.g., spent nuclear fuel, due to high radiation levels. Microfluidic E-cells offer advantages such as reduced radiation exposure, control over fluid flow rates, and high-throughput capabilities.Methods: The design of the E-cell considers electrode morphology, adhesion to a thin membrane, electrode configuration, and vacuum compatibility. Three techniques, including FIB-SEM lift-out, Au coating, and polyvinylidene fluoride (PVDF) binder, are explored for fabricating and attaching microgram quantities of UO2 as working electrodes. The PVDF binder method proves to be the most effective, enabling the creation of a vacuum-compatible microfluidic E-cell.Results and discussion: The PVDF binder method demonstrates successful electrochemical responses and allows for real-time monitoring of UO2 electrode behavior at the microscale. It offers chemical imaging capabilities using in situ SEM/EDS analysis. The technique provides consistent redox outcomes similar to bulk electrochemical analysis.Conclusion: The development of a microgram-scale microfluidic electrochemical cell using the PVDF binder technique enables the investigation of UO2 redox behavior. It offers a low-risk approach with reduced radiation exposure and high-throughput capabilities. The technique provides real-time monitoring and chemical imaging capabilities, making it valuable for studying spent nuclear fuel systems and material characterization.
An integrated radiomics nomogram based on conventional ultrasound improves discriminability between fibroadenoma and pure mucinous carcinoma in breast
Authors: Wang Hui , Zha Hailing , Du Yu , Li Cuiying , Zhang Jiulou , Ye Xinhua 
Published: June 2023

ObjectiveTo evaluate the ability of integrated radiomics nomogram based on ultrasound images to distinguish between breast fibroadenoma (FA) and pure mucinous carcinoma (P-MC).MethodsOne hundred seventy patients with FA or P-MC (120 in the training set and 50 in the test set) with definite pathological confirmation were retrospectively enrolled. Four hundred sixty-four radiomics features were extracted from conventional ultrasound (CUS) images, and radiomics score (Radscore) was constructed using the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm. Different models were developed by a support vector machine (SVM), and the diagnostic performance of the different models was assessed and validated. A comparison of the receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA) was performed to evaluate the incremental value of the different models.ResultsFinally, 11 radiomics features were selected, and then Radscore was developed based on them, which was higher in P-MC in both cohorts. In the test group, the clinic + CUS + radiomics (Clin + CUS + Radscore) model achieved a significantly higher area under the curve (AUC) value (AUC = 0.86, 95% CI, 0.733-0.942) when compared with the clinic + radiomics (Clin + Radscore) (AUC = 0.76, 95% CI, 0.618-0.869, P &gt; 0.05), clinic + CUS (Clin + CUS) (AUC = 0.76, 95% CI, 0.618-0.869, P&lt; 0.05), Clin (AUC = 0.74, 95% CI, 0.600-0.854, P&lt; 0.05), and Radscore (AUC = 0.64, 95% CI, 0.492-0.771, P&lt; 0.05) models, respectively. The calibration curve and DCA also suggested excellent clinical value of the combined nomogram.ConclusionThe combined Clin + CUS + Radscore model may help improve the differentiation of FA from P-MC.
T-cell-derived TNF-α and a cluster of immunological parameters from plasma allow a separation between SARS-CoV-2 convalescent versus vaccinated elite athletes
Authors: Palmowski Jana , Kohnhorst Sarah , Bauer Pascal , Puta Christian , Haunhorst Simon , Gebhardt Kristina , Reichel Thomas , Keller Christian , Huber Magdalena , Raifer Hartmann , Krüger Karsten 
Published: June 2023

Guidelines for medical clearing after SARS-CoV-2 infection in elite athletes do not include T-cell immunity aspects despite its relevance in the course of COVID-19 disease. Therefore, we aimed to analyze T-cell-related cytokines before and after in-vitro activation of CD4+ T-cells. We sampled professional indoor sports athletes at medical clearing after SARS-CoV-2 infection obtaining clinical, fitness data, and serological data including CD4+ T-cell cytokines. All data were analyzed by principal component analysis and 2 × 2 repeated measures ANOVA. CD4+ T-cells were sampled for cell culture activation with anti-CD3/anti-CD28 tetramers. At medical clearing, CD4+ T-cells from convalescent athletes secreted increased levels of TNF-α 72 h after in-vitro activation compared to vaccinated athletes. IL-18 levels in plasma were elevated and a cluster of parameters differentiated convalescent from vaccinated athletes by 13 parameters at the timepoint of medical clearing. All clinical data indicate infection is resolved, while increased TNF-α may reflect altered proportions of peripheral T-cells as a hangover of infection.
Understanding psychoanalytic work online and back to the couch in the wake of the COVID-19 pandemic: an investigation among Italian psychoanalysts
Authors: Reatto Licia Lea , Werbart Andrzej , Oasi Osmano , De Salve Francesca , Ierardi Elena , Giordano Mattia , Riva Crugnola Cristina 
Published: June 2023

BackgroundWorldwide, psychotherapists’ clinical experience went through rapid developments with transition to teletherapy during the COVID-19 pandemic. Literature on the use of remote psychoanalysis was not conclusive, leaving the issue of the consequences of the necessary setting alternation open. This study aimed to investigate the psychoanalysts’ experiences of shifting to remote work and then returning to in-person setting, considering the effect of the patients’ attachment styles and personality configurations.MethodSeventy-one analysts of the Italian Psychoanalytic Society were asked to fill out an online survey about patients who found the transition easier and patients who found it more difficult. General questions on therapeutic work, ISTS (Interpretive and Supportive Technique Scale) for interpretive and supportive aspects of technique, WAI-S-TR (Working Alliance Inventory-Short Revised-Therapist) for therapeutic alliance, RQ (Relationship Questionnaire) for attachment style, and PMAI (Prototype Matching of Anaclitic-Introjective Personality Configuration) for personality configurations were administered.ResultsAll of the analysts chose to continue the treatment using audio-visual tools. Patients with difficult transitions had a significantly higher frequency of insecure attachment and a higher score on RQ Dismissing scale than patients with easy transitions. No significant differences were found between the two groups in personality configurations, psychotherapeutic alliance, and psychotherapeutic technique. Moreover, a higher level of therapeutic alliance was positively correlated to RQ Secure scale and was negatively correlated to RQ Dismissing scale. Patients with easy transition both to remote work and back to in-person setting had higher scores of therapeutic alliances than those with difficult transition both to remote work and back to in-person setting.ConclusionOnline psychoanalytic therapy was widely used during the COVID-19 pandemic. Patients with insecure attachment styles had greater difficulties in adapting to setting alternations, thus confirming that insecure attachment is a vulnerability factor not only for psychopathological problems but also for a well-functioning therapeutic collaboration. Patient’s personality configuration did not influence their adaptation to the setting alternation. The supportive and interpretive styles did not undergo significant changes in the transition from in-person setting to remote setting and vice versa, thus suggesting a continuity in the analysts’ “internal setting.”
The association between psychological distress and alcohol consumption and physical activity: a population-based cohort study
Authors: Alpers Silvia Eiken , Pallesen Ståle , Vold Jørn Henrik , Haug Ellen , Lunde Linn-Heidi , Skogen Jens Christoffer , Mamen Asgeir , Mæland Silje , Fadnes Lars Thore 
Published: June 2023

IntroductionThe COVID-19 pandemic and infection control measures caused changes to daily life for most people. Heavy alcohol consumption and physical inactivity are two important behavioral risk factors for noncommunicable diseases worldwide. The COVID-19 pandemic, with its social distancing measures, home office policies, isolation, and quarantine requirements may have an impact on these factors. This three-wave longitudinal study aims to investigate if psychological distress and worries related to health and economy were associated with levels and changes in alcohol consumption and physical activity during the two first years of the COVID-19 pandemic in Norway.MethodsWe used data collected in April 2020, January 2021, and January 2022 from an online longitudinal population-based survey. Alcohol consumption and physical activity status were assessed at all three measuring points via the Alcohol Use Disorder Identification Test (AUDIT-C) and the International Physical Activity Questionnaire (IPAQ-SF). COVID-19-related worries, home office/study, occupational situation, age, gender, children below 18 years living at home, and psychological distress (measured with the Symptom Checklist (SCL-10)) were included as independent variables in the model. A mixed model regression was used and presented with coefficients with 95% confidence intervals (CI).ResultsAnalysis of data from 25,708 participants demonstrates that participants with substantial symptoms of psychological distress more often reported higher alcohol consumption (1.86 units/week, CI 1.48–2.24) and lower levels of physical activity [−1,043 Metabolic Equivalents of Task (METs) per week, CI −1,257;−828] at baseline. Working/studying from home (0.37 units/week, CI 0.24–0.50) and being male (1.57 units/week, CI 1.45–1.69) were associated with higher alcohol consumption. Working/studying from home (−536 METs/week, CI −609;−463), and being older than 70 years (−503 METs/week, CI −650;−355) were related to lower levels of physical activity. The differences in activity levels between those with the highest and lowest levels of psychological distress reduced over time (239 METs/week, CI 67;412), and similarly the differences in alcohol intake reduced over time among those having and not having children &lt; 18 years (0.10 units/week, CI 0.01–0.19).ConclusionThese findings highlight the substantial increases in risks related to inactivity and alcohol consumption among those with high levels of psychological distress symptoms, and particularly during the COVID-19 pandemic, and increase the understanding of factors associated with worries and health behavior.
Molecular Characterization and Haplotype Analysis of Low Phytic Acid-1 (lpa1) Gene Governing Accumulation of Kernel Phytic Acid in Subtropically-Adapted Maize
Authors: Bhatt Vinay , Muthusamy Vignesh , Chhabra Rashmi , Katral Ashvinkumar , Ragi Shridhar , Rojaria Vinay , Chand Gulab , Sarma Govinda Rai , Zunjare Rajkumar Uttamrao , Panda Kusuma Kumari , Singh Ashok Kumar , Hossain Firoz 
Published: June 2023

Maize is an important food, feed, fodder and industrial crop in addition to being a valuable source of micronutrients. Phytic acid (PA), an anti-nutritional factor in maize, makes crucial minerals inaccessible to monogastric animals. The low phytic acid-1 (lpa1) gene located on chromosome-1S is 7292 bp long with 11 exons, and the recessive lpa1-1 allele reduces the accumulation of PA thereby enhances the bioavailability of essential minerals in maize kernels. Here, we characterized the full-length Lpa1 gene sequence in three mutants (lpa1-1) and seven wild-type (Lpa1) maize inbreds. Sequence analysis revealed 607 polymorphic sites across Lpa1 sequences, indicating wide variability for Lpa1 among the inbreds. Further, SNP from “C” to “T” differentiated wild-type and mutant-type alleles at 1432 amino acid position. Gene-based diversity among 48 diverse maize inbreds using 15 InDel markers revealed the formation of 42 distinct haplotypes; six of which (Hap6, Hap16, Hap17, Hap19 Hap27 and Hap31) were shared by more than one genotype. The number of exons in Lpa1 ranged from 11–19 among maize genotypes and 6–14 among 26 orthologues. Major functional motifs of Lpa1 detected were ATP-binding Cassette (ABC) transporter trans-membrane region and ABC transporter. Phylogenetic tree using nucleotide and protein sequences revealed a closer relationship of maize Lpa1 sequences with Sorghum bicolor, Panicum hallii, Setaria italica and S. viridis. This study offered newer insights into the understanding of the genetic diversity of the Lpa1 gene in maize and related crop-species, and information generated here would further help in exploiting the lpa1 mutant for the enhancement of nutritional value in maize kernels.
Typifications and Nomenclatural Notes on Old World Names in Boehmeria (Urticaceae)
Authors: Jeomol K. K. , Sunojkumar P. 
Published: June 2023

Lectotypes are designated here for nine names in the genus Boehmeria Jacq.: B. depauperata Wedd., B. humilis Miq., B. malabarica Wedd., B. pilosiuscula (Blume) Hassk., B. subperforata Wedd., B. travancorica Bedd., B. virgata (G. Forst.) Guill. var. longissima (Hook. f.) Friis & Wilmot-Dear, B. virgata var. macrostachya (Wight) Friis & Wilmot-Dear, and B. zollingeriana Wedd. Nomenclatural changes to names in the recently published taxonomic revision of the Old World species of the genus Boehmeria are also discussed: B. glomerulifera Miq. and B. diversiflora Miq. are considered homotypic and superfluous to B. depauperata and B. zollingeriana, respectively; B. malabarica represents a superfluous and illegitimate renaming of B. depauperata but is not automatically homotypic with the latter; and Ramium malabaricum Kuntze is added to the synonymy of B. depauperata.
Multimodal Visualization of Ophthalmic Manifestations of Long-COVID-19. Clinical Case
Authors: Юсеф Н.Ю. , Анджелова Д.В. , Казарян Э.Э. , Воробьева М.В. 
Published: June 2023

В эпоху эпидемии коронавирусной инфекции 2019 г. (COVID-19) и борьбы с вирусом SARS-CoV-2 резко возросло число случаев глазной патологии, в частности воспалительного характера. Причиной тому может быть как сама коронавирусная инфекция, так и активная иммунизация населения вакцинами. Вакцина как триггер способна запускать каскад аутоиммунных реакций с развитием воспалительного ответа. Описано немало побочных явлений вакцинации против SARS-CoV-2. Частым осложнением перенесенной коронавирусной инфекции и вакцинации является воспаление сосудистой оболочки глаза. Значительные и порой необратимые зрительные нарушения вследствие аутоиммунного ответа, обусловленного вакцинацией или коронавирусной инфекцией, требуют высокой осведомленности врачей о возможных офтальмологических проявлениях. В статье описывается случай отдаленных офтальмологических проявлений перенесенной инфекции COVID-19 на фоне предшествующей ей иммунизации вакциной Pfizer/BioNTech (BNT162b2) против данной вирусной инфекции. С помощью высокотехнологичных методов исследования выявлены и описаны изменения заднего отрезка глаза вследствие перенесенного ранее увеита. Клинический случай демонстрирует, что COVID-19 и вакцинация против него, предположительно, могут играть роль в индукции иммунного ответа с развитием воспалительного процесса глаз.
In the era of the epidemic of coronavirus infection in 2019 (COVID-19) and the fight against the SARS-CoV-2 virus, the number of cases of ocular pathology, in particular of an inflammatory nature, has sharply increased. The reason for this may be both the coronavirus infection itself and the active immunization of the population with vaccines. The vaccine as a trigger is able to trigger a cascade of autoimmune reactions with the development of an inflammatory response. Many side effects of vaccination against SARS-CoV-2 have been described. A common complication of a coronavirus infection and vaccination is inflammation of the vascular membrane of the eye. Significant and sometimes irreversible visual disturbances due to an autoimmune response caused by vaccination or coronavirus infection require high awareness of doctors about possible ophthalmological manifestations. The article describes a case of distant ophthalmological manifestations of a COVID-19 infection against the background of a previous vaccination Pfizer/BioNTech (BNT162b2) against this viral infection. With the help of high-tech research methods, changes in the posterior segment of the eye due to previously transferred uveitis have been identified and described. A clinical case demonstrates that COVID-19 and vaccination against it, presumably, can play a role in the induction of an immune response with the development of an inflammatory of eye.