bluuebunny/crossref_metadata_2025_split huggingface dataset:
Energy storage complementary control method for wind‐solar storage combined power generation system under opportunity constraint
Authors: Tian Xin 
Published: April 2023

AbstractDue to the different complementarity and compatibility of various components in the wind‐solar storage combined power generation system, its energy storage complementary control is very important. In order to ensure the stable operation of the system, an energy storage complementary control method for wind‐solar storage combined power generation system under opportunity constraints is proposed. The wind power output value is obtained. Aiming at the maximum similarity between the total output of wind power storage and the planned output curve, combined with the opportunity constraints and the output and electric quantity constraints of energy storage devices, the energy storage complementary control model is established, and the model is solved by improving the adaptive particle swarm optimization algorithm. The experimental results show that the total output of the wind‐solar storage combined power generation system is consistent with the expected output, and the utilization rate of wind‐solar resources is effectively improved. It has good convergence performance, and can better meet the needs of actual power work.
Assessing the significance of first place and online third places in supporting Malaysian seniors’ well-being during the pandemic
Authors: Tan Teck Hong , Idris Izian 
Published: April 2023

AbstractThe enforced lockdowns and social distancing measures associated with COVID-19 may have influenced older adults’ preferences towards their homes and neighborhoods as well as social spaces. One objective of this research is to determine whether home and neighborhood environments (“first place”) affect how satisfied older adults are with their lives during the epidemic. This study also examined the extent to which social spaces that exist in the virtual world (“online third places”) affect older adults’ life satisfaction when they would have to practice risk-averse behaviors in times of pandemic. To collect data, this study analyzed the responses of 500 active older adults and conducted in-depth interviews with seven older adults who served as neighborhood leaders in Klang Valley, Malaysia. The study found that there is a direct relationship between older adults’ satisfaction with their current housing and their overall life satisfaction during the pandemic. Similarly, having a quality neighborhood nearby increases the likelihood of living a satisfied life during the pandemic. Most online third places, with the exception of instant messaging apps, do not appear to provide older adults with an adequate platform to interact with their friends, participate in social networking, and join communities for emotional support during the pandemic. The findings and recommendations of this study would be very useful in developing effective interventions to promote aging in place during the coronavirus outbreak.
(p)ppGpp – an important player during heat shock response
Authors: Driller Kristina , Cornejo Fabián A , Turgay Kürşad 
Published: April 2023

AbstractThe alarmones and second messengers (p)ppGpp are important for the cellular response to amino acid starvation. Although the stringent response is present in many bacteria, the targets and functions of (p)ppGpp can differ between species, and our knowledge of (p)ppGpp targets is constantly expanding. Recently, it was demonstrated that these alarmones are also part of the heat shock response in Bacillus subtilis and that there is a functional overlap with the oxidative and heat stress transcriptional regulator Spx. Here, the (p)ppGpp second messenger alarmones allow the fast stress-induced downregulation of translation while Spx inhibits the further expression of translation-related genes to lower the load on the protein quality control system, while the chaperone and protease expression is induced. In this review, we discuss the role of (p)ppGpp and its intricate connections in the complex network of stress sensing, heat shock response, and adaptation in B. subtilis cells.
The future of ocean plastics: designing diverse collaboration frameworks
Authors: Mofokeng R P , Faltynkova A , Alfonso M B , Boujmil I , Carvalho I R B , Lunzalu K , Zanuri N B Mohd , Nyadjro E S , Puskic P S , Lindsay D J , Willis K , Adyel T M , Serra-Gonçalves C , Zolich A , Eriksen T S , Evans H-C , Gabriel D , Hajbane S , Suaria G , Law K L , Lobelle D 
Published: April 2023

Abstract
This paper aims to guide the stakeholder engagement process related to plastic pollution research in marine environments. We draw on advice identified during an online workshop (Ocean Plastic Workshop 2022) organized by Early Career Ocean Professionals (ECOPs) from 11 countries, held in April 2022. International experts and workshop participants discussed their experiences in the collaborative development and implementation of ocean plastic pollution projects held worldwide, guided by three main questions: (i) What is the role of scientists in a multi-stakeholder project? (ii) How should scientists communicate with other stakeholders? (iii) Which stakeholders are missing in collaborative projects, and why are they missing? This multidisciplinary, co-learning approach highlights the value of stakeholder engagement for ocean plastic projects with an end goal to identify and implement ocean plastic solutions via innovative technologies, informing policy, community engagement, or a combination of all three approaches. The target outcomes of the workshop described in this paper include the identification of transdisciplinary (academic-stakeholder) engagement frameworks and specific suggestions that can serve as guidelines for the development of future plastic pollution projects.
Nutritional value of a new source of cheese coproduct fed to weanling pigs
Authors: Mallea Andrea P , Oliveira Maryane S F , Lopez Diego A , Stein Hans H 
Published: April 2023

AbstractThree experiments were conducted to test the hypothesis that values for standardized ileal digestibility (SID) of amino acids (AA) and metabolizable energy (ME) in a the cheese coproduct are greater than in fish meal or enzyme-treated soybean meal (ESBM). The second objective was to test the hypothesis that pigs fed a diet containing cheese coproduct will have growth performance that is not different from that of pigs fed other sources of protein. In experiment 1, eight ileal-cannulated barrows (11.0 ± 0.4 kg) were allotted to a replicated 4 × 4 Latin square design with four diets and four periods and two pigs per diet in each period. The four diets included an N-free diet and three diets that contained ESBM, fish meal, or the cheese coproduct as the source of AA. Results indicated that the cheese coproduct had greater (P &lt; 0.05) SID of most AA compared with ESBM and fish meal. In experiment 2, 32 weanling barrows (14.0 ± 1.1 kg) were housed individually in metabolism crates and randomly allotted to one of four diets. A corn-based diet and three diets that contained corn and ESBM, fish meal, or cheese coproduct were formulated. Feces and urine were collected quantitatively. The ME in cheese coproduct was greater (P &lt; 0.05) than in ESBM and fish meal. In experiment 3, 128 weaned pigs (6.2 ± 0.6 kg) were allotted to a randomized complete block design with four treatments and 8 replicate pens per diet. Phase 1 diets that contained 0%, 6.65%, 7.35%, or 14% cheese coproduct were fed from days 1 to 14 and a common phase 2 diet without cheese coproduct was fed from days 15 to 28. Individual pig weights were recorded at the beginning of the experiment, on days 14 and 28, and daily feed allotments were also recorded. Two blood samples were collected from 1 pig per pen on day 14 to analyze for blood urea N, albumin, total plasma protein, peptide YY, immunoglobulin G, tumor necrosis factor-α, interleukin-6, and interleukin-10. No differences were observed in average daily gain among treatments, but there was a tendency (P &lt; 0.10) for total protein on day 14 to increase as cheese coproduct increased in the diets. In conclusion, the cheese coproduct used in this experiment has a greater SID of AA and greater ME than ESBM and fish meal and the cheese coproduct may be included in prestarter diets for weanling pigs without negatively impacting growth performance or indicators of intestinal health.
Challenges and advances towards the rational design of microalgal synthetic promoters in Chlamydomonas reinhardtii
Authors: Milito Alfonsina , Aschern Moritz , McQuillan Josie L , Yang Jae-Seong 
Published: April 2023

Abstract
Microalgae hold enormous potential to provide a safe and sustainable source of high-value compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly progressing climate change. Bioengineering microalgal strains will be key to optimizing and modifying their metabolic outputs, and to render them competitive with established industrial biotechnology hosts, such as bacteria or yeast. To achieve this, precise and tuneable control over transgene expression will be essential, which would require the development and rational design of synthetic promoters as a key strategy. Among green microalgae, Chlamydomonas reinhardtii represents the reference species for bioengineering and synthetic biology; however, the repertoire of functional synthetic promoters for this species, and for microalgae generally, is limited in comparison to other commercial chassis, emphasizing the need to expand the current microalgal gene expression toolbox. Here, we discuss state-of-the-art promoter analyses, and highlight areas of research required to advance synthetic promoter development in C. reinhardtii. In particular, we exemplify high-throughput studies performed in other model systems that could be applicable to microalgae, and propose novel approaches to interrogating algal promoters. We lastly outline the major limitations hindering microalgal promoter development, while providing novel suggestions and perspectives for how to overcome them.
UVIT view of NGC 5291: Ongoing star formation in tidal dwarf galaxies at ∼ 0.35 kpc resolution
Authors: Rakhi R , Santhosh Geethika , Joseph Prajwel , George Koshy , Subramanian Smitha , Kavila Indulekha , Postma J , Duc Pierre-Alain , Côté Patrick , Cortese Luca , Ghosh S K , Subramaniam Annapurni , Tandon Shyam , Hutchings John , Wesley P Samuel , Bharadwaj Aditya , Niroula Neeran 
Published: April 2023

ABSTRACT
NGC 5291, an early-type galaxy surrounded by a giant H i ring, is believed to be formed from collision with another galaxy. Several star forming complexes and tidal dwarf galaxies are distributed along the collisional ring which are sites of star formation in environments where extreme dynamical effects are involved. Dynamical effects can affect the star formation properties and the spatial distribution of star forming complexes along the tidal features. To study and quantify the star formation activity in the main body and in the ring structure of the NGC 5291 system, we use high spatial resolution FUV and NUV imaging observations from the Ultraviolet Imaging Telescope onboard AstroSat. A total of 57 star-forming knots are identified to be part of this interacting system out of which 12 are new detections (star forming complexes that lie inside the H i contour) compared to the previous measurements from lower resolution UV imaging. We estimate the attenuation in UV for each of the resolved star-forming knots using the UV spectral slope β, derived from the FUV − NUV colour. Using the extinction corrected UV fluxes, we derive the star formation rate of the resolved star forming complexes. The extinction corrected total star formation rate of this system is estimated as 1.75 ± 0.04 $\mathrm{M_\odot \, yr}^{-1}$. The comparison with dwarf galaxy populations (BCD, Sm, and dIm galaxies) in the nearby Universe shows that many of the knots in the NGC 5291 system have SFR values comparable to the SFR of BCD galaxies.
Relationship between the phase angle from bioelectrical impedance analysis and daily physical activity in patients with rheumatoid arthritis
Authors: Matsumoto Yoshinari , Tada Masahiro , Yamada Yutaro , Mandai Koji , Hidaka Noriaki 
Published: April 2023

ABSTRACT

Objectives
This study aimed to examine the relationship between daily physical activity and the phase angle (PhA) obtained by bioelectrical impedance analysis in rheumatoid arthritis (RA) patients.

Methods
Data from a prospective cohort study of RA patients who were surveyed every year were analysed. The PhA was assessed by the bioelectrical impedance analysis method, and physical activity was assessed as the amount of time of exercise in metabolic equivalents (METs) per day using a triaxial accelerometer for 7 consecutive days. The association between physical activity and the PhA was evaluated using the isotemporal substitution model in multiple regression analysis.

Results
Seventy-six RA patients were included in the analysis (81% female and age 66.2 ± 13.1 years). On cross-sectional analysis, the isotemporal substitution model in multiple regression analysis showed that the PhA was 0.05 points higher every 10 minutes when activities with intensity of 1 ≤ METs &lt; 2 were replaced by activities with intensity of ≥3 METs (P = .01). Over 1 year, the rate of change in the PhA was 0.69% higher every 10 minutes when activities with intensity of 1 ≤ METs &lt; 2 were replaced by activities with intensity of ≥3 METs (P = .037).

Conclusion
The PhA in RA patients may be related to physical activity level.
Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation
Authors: Lucky Amuza B , Wang Chengqi , Li Xiaolian , Chim-Ong Anongruk , Adapa Swamy R , Quinlivan Eoin P , Jiang Rays , Cui Liwang , Miao Jun 
Published: April 2023

Abstract
DNA modifications are critical in fine-tuning the biological processes in model organisms. However, the presence of cytosine methylation (5mC) and the function of the putative DNA methyltransferase, PfDNMT2, in the human malaria pathogen, Plasmodium falciparum, remain controversial. Here, we revisited the 5mC in the parasite genome and the function of PfDNMT2. Low levels of genomic 5mC (0.1–0.2%) during asexual development were identified using a sensitive mass spectrometry procedure. Native PfDNMT2 displayed substantial DNA methylation activities, and disruption or overexpression of PfDNMT2 resulted in reduced or elevated genomic 5mC levels, respectively. PfDNMT2 disruption led to an increased proliferation phenotype, with the parasites having an extended schizont stage and producing a higher number of progenies. Consistent with PfDNMT2’s interaction with an AP2 domain-containing transcription factor, transcriptomic analyses revealed that PfDNMT2 disruption led to a drastic alteration in the expression of many genes, some of which provided the molecular basis of enhanced proliferation after PfDNMT2 disruption. Furthermore, levels of tRNAAsp and its methylation rate at position C38, and the translation of a reporter containing an aspartate repeat were significantly reduced after PfDNMT2 disruption, while the levels of tRNAAsp and its C38 methylation were restored after complementation of PfDNMT2. Our study sheds new light on the dual function of PfDNMT2 during P. falciparum asexual development.
Requirement of transcription-coupled nucleotide excision repair for the removal of a specific type of oxidatively induced DNA damage
Authors: Sarmini Leen , Meabed Mohammed , Emmanouil Eirini , Atsaves George , Robeska Elena , Karwowski Bolesław T , Campalans Anna , Gimisis Thanasis , Khobta Andriy 
Published: April 2023

Abstract
Accumulation of DNA damage resulting from reactive oxygen species was proposed to cause neurological and degenerative disease in patients, deficient in nucleotide excision repair (NER) or its transcription-coupled subpathway (TC-NER). Here, we assessed the requirement of TC-NER for the repair of specific types of oxidatively generated DNA modifications. We incorporated synthetic 5′,8-cyclo-2′-deoxypurine nucleotides (cyclo-dA, cyclo-dG) and thymine glycol (Tg) into an EGFP reporter gene to measure transcription-blocking potentials of these modifications in human cells. Using null mutants, we further identified the relevant DNA repair components by a host cell reactivation approach. The results indicated that NTHL1-initiated base excision repair is by far the most efficient pathway for Tg. Moreover, Tg was efficiently bypassed during transcription, which effectively rules out TC-NER as an alternative repair mechanism. In a sharp contrast, both cyclopurine lesions robustly blocked transcription and were repaired by NER, wherein the specific TC-NER components CSB/ERCC6 and CSA/ERCC8 were as essential as XPA. Instead, repair of classical NER substrates, cyclobutane pyrimidine dimer and N-(deoxyguanosin-8-yl)-2-acetylaminofluorene, occurred even when TC-NER was disrupted. The strict requirement of TC-NER highlights cyclo-dA and cyclo-dG as candidate damage types, accountable for cytotoxic and degenerative responses in individuals affected by genetic defects in this pathway.
Lumbar Arthroplasty Is Associated With a Lower Incidence of Adjacent Segment Disease Compared With ALIF
Authors: Shukla Geet G. , Wu Andrew , Matur Abhijith V. , McGrath Kyle , Khalid Syed , Garner Rebecca , Owen Bryce , Ivey Natalie , Vorster Phillip , Onyewadume Louisa , Tao Xu , Motley Benjamin , Cheng Joseph , Adogwa Owoicho 
Published: April 2023

Study Design.
Retrospective Cohort Study

Objective.
The objective of this study was to compare the rate of adjacent segment disease (ASD) between lumbar disk arthroplasty (LDA) and anterior lumbar interbody fusion (ALIF).

Summary of Background Data.
LDA and ALIF are alternative surgical approaches used to treat lumbar degenerative disk disease. However, there is a paucity of studies comparing the risk of ASD after these procedures.

Methods.
Patients who underwent 1- to 2-level LDA or ALIF between 2010 and 2022 were identified in the PearlDiver Mariner insurance all-claims database. Exclusion criteria included the history of prior lumbar spine surgery or surgery for tumors, trauma, or infection. 1:1 propensity matching was performed using demographic factors, medical comorbidities, and surgical factors, which were significantly associated with ASD.

Results.
1:1 propensity matching created two equal groups of 1625 patients without baseline differences who underwent LDA or ALIF. LDA was significantly associated with a lower risk of ASD (relative risk: 0.932, 95% CI, 0.899–0.967, P<0.001) and need for revision within 30 days (relative risk: 0.235, 95% CI, 0.079–0.698, P=0.007). There were no differences in all-cause surgical and medical complications between both groups.

Conclusions.
After risk adjustment for demographic and clinical characteristics, the results suggest that LDA is associated with a lower risk of ASD compared with ALIF. LDA was also associated with lower hospital cost and shorter length of stay.
10-year Longitudinal MRI Study of Intervertebral Disk Degeneration in Patients With Lumbar Spinal Canal Stenosis After Posterior Lumbar Decompression Surgery
Authors: Fujii Takeshi , Daimon Kenshi , Ozaki Masahiro , Suzuki Satoshi , Takahashi Yohei , Tsuji Osahiko , Nagoshi Narihito , Yagi Mitsuru , Michikawa Takehiro , Matsumoto Morio , Nakamura Masaya , Watanabe Kota 
Published: April 2023

Study Design.
A prospective longitudinal magnetic resonance imaging (MRI) study.

Objective.
The objective of this study was to describe the progression of intervertebral disk (IVD) degeneration in patients who underwent posterior decompression surgery for lumbar spinal canal stenosis (LSS).

Summary of Background Data.
IVD degeneration contributes to the pathogenesis of LSS; however, the long-term consequences of degenerative changes after decompression surgery remain unknown.

Materials and Methods.
Of 258 consecutive patients who underwent posterior lumbar decompression surgery for LSS, 62 who underwent MRI at their 10-year follow-up were included; 17 age-matched asymptomatic volunteers were analyzed as controls. Three MRI findings representing IVD degeneration were graded on their severity: decrease in signal intensity, posterior disk protrusion (PDP), and disk space narrowing (DSN). Clinical outcome was assessed using the low back pain (LBP) score from the Japanese Orthopaedic Association scoring system. We examined the association between the progression of degenerative changes on MRI and LBP/associated factors using logistic regression adjusting for age at baseline and sex.

Results.
The severity of IVD degeneration tended to be higher in patients with LSS than asymptomatic volunteers at both baseline and follow-up. IVD degeneration progressed in all patients during the 10-year follow-up period. Progression of decrease in signal intensity and PDP was observed at L1/2 in 73% and at L2/3 in 34%, respectively (the highest frequencies in the lumbar spine). Progression of DSN was highest at L4/5 in 42%. The rates of PDP and DSN progression during the 10-year follow-up period tended to be greater in patients with LSS than in asymptomatic volunteers. No significant difference in the proportion of LBP deterioration was evident for individuals with and without MRI findings of progression.

Conclusions.
Our study reveals a natural history of the long-term postoperative course of IVD degeneration after posterior decompression surgery for LSS. Compared with healthy controls, patients with LSS seemed to be predisposed to IVD degeneration. Lumbar decompression surgery may promote the progression of DSN; however, progression of IVD degeneration after lumbar decompression surgery was not associated with worsening LBP scores.
The Role of Antibiotics in Nasal Fractures after Closed Reduction
Authors: Vishwanath Neel , Rhee Ben , Sobti Nikhil , Beqiri Dardan , Xi Kevin , Lerner Julia , Woo Albert S 
Published: April 2023

Background:
Nasal fractures represent the most common fracture in facial trauma. The role of prophylactic antibiotics in these injuries is debated, given low infection rates and demonstrated risks of antibiotics. We studied the isolated effect of prophylactic antibiotics on infection rate in patients with nasal fracture after closed reduction.

Methods:
Retrospective cohort study of a prospectively maintained facial trauma database was conducted. Demographics, comorbidities, fracture classifications, and management of patients who received antibiotics at the time of closed nasal reduction were compared against those who did not receive antibiotics. Infection rates between groups were analyzed. Multivariate analysis was conducted to control for confounding variables. Qualitative analysis was performed for patients who experienced infection following nasal fracture.

Results:
A total of 282 patients met inclusion criteria (n = 144, antibiotic; n = 138, nonantibiotic). Six patients experienced infection. There was no difference in infection rate between antibiotic and nonantibiotic groups (2.0% versus 2.2%; P = 0.90). On multivariate regression, antibiotics did not significantly decrease odds of infection (OR 1.7 [0.17–13.6]; P = 0.64). Moreover, patients with open nasal fractures did not have significantly higher odds of infection (OR 1.9 [0.08–20.8]; P = 0.64). Similarly, increasing severity of injury based on Rohrich classification did not significantly impact odds of infection (OR 0.68 [0.23–1.9]; P = 0.46). All six infections were managed at the bedside, with zero infections following operating room management (P = 0.32).

Conclusions:
Prophylactic antibiotics do not decrease infection rates following nasal fractures managed by closed reduction. Bedside management may be a risk factor for the development of infection; however, this finding requires further evaluation.