bluuebunny/crossref_metadata_2025_split huggingface dataset:
What drives young workers? Person‐organization fit‐in identity motives—A mixed‐method study
Authors: Manzi Claudia , Gorli Mara , Reverberi Eleonora , Crapolicchio Eleonora , Regalia Camillo , Valenzisi Pamela 
Published: August 2023

AbstractPerson‐organization fit theory is among the most productive theoretical frameworks used to assess the relationship between an organization and its employees. Nevertheless, the P‐O fit literature has often limited its scope to the evaluation of P‐O fit in the values domain, overlooking P‐O fit‐in needs. This study addresses this issue under the motivated identity construction theory (MICT, Vignoles, Handbook of identity theory and research, New York: Springer; 2011). In particular, we analyse how the fit between individual identity's needs and the organization identity's needs may influence the retention and engagement of under 35 workers. Findings demonstrated that the need for efficacy is fundamental for workers under 35 s. Not only this aspect of motivation is rated as more important in comparison with others but also P‐O fit‐in efficacy has a direct impact on identification with the company and a direct and indirect effect (through organizational identification) on engagement and intention to leave. Overall, this study gives evidence of how useful the intersection between P‐O fit with MICT can be. Please refer to the Supplementary Material section to find this article's Community and Social Impact Statement.
The effect of repeat purchase information in electronic word‐of‐mouth (eWOM) on purchase intention
Authors: Li Shang , Chen Jie , Chen Yiwen 
Published: August 2023

AbstractConsumers commonly rely on electronic word‐of‐mouth (eWOM) to inform their purchase decisions. The purpose of this study is to investigate when and why repeat purchase information in eWOM influenced customers' purchase behavior, using the social learning theory as a framework. Three experiments were conducted to test the hypotheses. The results showed that repeat purchase information in eWOM positively affected consumers' purchase intention through perceived diagnosticity of eWOM and perceived value. Additionally, the effect varied from product type. For utilitarian products, the repeat purchase information in eWOM had a significant impact on consumers' perceived diagnosticity and purchase intention, while for hedonic products, the impact was not significant. The current study deepens the understanding of social learning theory in eWOM content by providing a novel look at repeat purchase information in eWOM. These findings offer important implications for both research and practical applications of eWOM, particularly in term of effectively utilizing repeat purchase information of eWOM.
Association of hypertension severity and control with risk of incident atrial fibrillation: The REasons for Geographic And Racial Differences in Stroke (REGARDS) study
Authors: Garg Parveen K. , Wilson Nicole , Yuan Ya , Howard Virginia J. , Judd Suzanne , Howard George , Soliman Elsayed Z. 
Published: August 2023

AbstractBackgroundThe association of hypertension (HTN) severity and control with the risk of incident atrial fibrillation (AF) is unclear.HypothesisIncreased HTN severity and poorer blood pressure control would be associated with an increased risk of incident AF.MethodsThis analysis included 9485 participants (mean age 63 ± 8 years; 56% women; 35% Black). Participants were stratified into six mutually exclusive groups at baseline—normotension (n = 1629), prehypertension (n = 704), controlled HTN (n = 2224), uncontrolled HTN (n = 4123), controlled apparent treatment‐resistant hypertension (aTRH) (n = 88), and uncontrolled aTRH (n = 717). Incident AF was ascertained at the follow‐up visit, defined by either electrocardiogram or self‐reported medical history of a physician diagnosis. Multivariable logistic regression analyses adjusted for demographic and clinical variables.ResultsOver an average of 9.3 years later, 868 incident AF cases were detected. Compared to those with normotension, incident AF risk was highest for those with aTRH (controlled aTRH: odds ratio (OR) 2.95; 95% confidence interval (CI) 1.60, 5.43, & uncontrolled aTRH: OR 2.47; 95% CI 1.76, 3.48). The increase in AF risk was smaller for those on no more than three antihypertensive agents regardless of their blood pressure control (controlled OR 1.72; 95% CI 1.30, 2.29 and uncontrolled OR 1.56; 95% CI 1.14, 2.13).ConclusionsThe risk of developing AF is increased in all individuals with HTN. Risk is highest in those aTRH regardless of blood pressure control. A more aggressive approach that focuses on lifestyle and pharmacologic measures to either prevent HTN or better control HTN during earlier stages may be particularly beneficial in reducing related AF risk.
Serial Polarization Transfer by Combination of Cross‐Relaxation and Rotational Resonance for Sensitivity‐Enhanced Solid‐State NMR
Authors: Biedenbänder Thomas , Bensons Edvards R. , Corzilius Björn 
Published: August 2023

AbstractThe front cover artwork is provided by Prof. Björn Corzilius's group at the University of Rostock. The image shows the interference of the isotropic frequency difference between two nuclear spins with the evolution of their dipolar coupling in an MAS rotor. This rotational resonance (R2) can be exploited for site‐specific dynamic nuclear polarization. Read the full text of the Research Article at 10.1002/cphc.202300206.
Flow Cytometry Method Validation Protocols
Authors: Selliah Nithianandan , Nash Veronica , Eck Steven , Green Cherie , Oldaker Teri , Stewart Jennifer , Vitaliti Alessandra , Litwin Virginia 
Published: August 2023

AbstractAnalytical method validation provides a means to ensure that data are credible and reproducible. This article will provide a brief introduction to analytical method validation as applied to cellular analysis by flow cytometry, along with practical procedures for four different types of validation. The first, Basic Protocol 1 (the limited validation protocol), is recommended for research and non‐regulated laboratories. Next, Basic Protocol 2) presents a reasonable, fit‐for‐purpose validation approach appropriate for biopharma and research settings. Basic Protocol 3 addresses the type of validation performed in clinical laboratories for moderate‐risk tests developed in house. Finally, Basic Protocol 4 describes the process that should be applied whenever a method is being transferred from one facility to another. All four validation plans follow the fit‐for‐purpose validation approach, in which the validation parameters are selected based on the intended use of the assay. These validation protocols represent the minimal requirement and may not be applicable for every intended use such as high‐risk clinical assays or data to be used as a primary endpoint in a clinical trial. The recommendations presented here are consistent with the white papers published by the American Association of Pharmaceutical Scientists and the International Clinical Cytometry Society, as well as with Clinical Laboratory Standards Institute Guideline H62: Validation of Assays Performed by Flow Cytometry (CLSI, 2021). © 2023 Wiley Periodicals LLC.Basic Protocol 1: Limited validationBasic Protocol 2: Fit‐for‐purpose validation for biopharma and research settingsBasic Protocol 3: Validation for moderate clinical risk laboratory developed testsBasic Protocol 4: Transfer validationThis article was corrected on 25 January 2024. See the end of the full text for details.
Sustainability transition strategies in a business context: A co‐creation process in the Portuguese hospitality sector
Authors: Tourais Patrícia , Videira Nuno 
Published: August 2023

AbstractThe role and impact of businesses in transition processes depend on the strategic positioning of firms regarding long‐term sustainability goals. Even though businesses are key actors for transition processes, the literature still lacks an extensive exploration of operational guidelines to support organisations in navigating the complexities of wider sustainability transitions. In this study, contributions from sustainability transitions research to the study of strategic planning processes in a business context were explored to address this gap. A procedure was developed to guide organisations towards development of sustainability transition strategies, which was further tested in a case study from the Portuguese tourism sector. The proposed approach builds on the transition management framework and a set of transversal sustainability elements: a holistic view of sustainability, long‐term thinking, stakeholder involvement and innovation. The testing of the proposed procedure resulted on the co‐creation of a sustainability transition strategy in a hotel which highlighted the need for participatory and long‐term oriented approaches to support businesses in their transformation processes. Improvement opportunities were identified regarding external stakeholder involvement and the integration of innovation elements while co‐creating organisational sustainability transition strategies.
Development of wrist‐watch type biosensing system for real‐time sweat lactate monitoring
Authors: Konno Sakae , Kudo Hiroyuki 
Published: August 2023

AbstractA wrist‐watch type biosensing system for real‐time sweat lactic acid (LA) monitoring was fabricated and tested. The system consists of a microfluidic LA biosensor, a carrier‐flow supplying device, and a sampling device for transporting whole secretions at the skin surface to the biosensor by continuous flow of phosphate‐buffered saline (PBS). The perfusion test of PBS onto the skin surface by using the sampling device resulted in less than 1% leakage frequency at the collection site. The carrier‐flow supplying device was able to feed PBS regardless of the body angle. When the subject wearing the device performed resistance training (lateral raise, 20 repetitions, 12.5 kg dumbbells), the LA secretion increased from 2 µg/cm2/min to 16 µg/cm2/min in response to the exercise, and then recovered to the baseline. The setup time for the measurement was less than 60 s. This indicates that the wrist‐watch device can readily measure LA dynamics on the skin surface under conditions of the subject.
Utility of left ventricular ejection fraction in atrial fibrillation patients without pre‐existing heart failure
Authors: Hamatani Yasuhiro , Iguchi Moritake , Minami Kimihito , Ishigami Kenjiro , Esato Masahiro , Tsuji Hikari , Wada Hiromichi , Hasegawa Koji , Ogawa Hisashi , Abe Mitsuru , Lip Gregory Y.H. , Akao Masaharu ,   the Fushimi AF Registry investigators
Published: August 2023

AbstractAimsAtrial fibrillation (AF) increases the risk of heart failure (HF); however, little focus has been placed on the prevention of HF in patients with AF. Left ventricular ejection fraction (LVEF) is an established echocardiographic parameter in HF patients. We sought to investigate the association of LVEF with HF events in AF patients without pre‐existing HF.Methods and resultsThe Fushimi AF Registry is a community‐based prospective survey of AF patients in Fushimi‐ku, Japan. In this analysis, we excluded patients with pre‐existing HF (defined as having one of the following: prior HF hospitalization, New York Heart Association class ≥ 2 in association with heart disease, or LVEF < 40%). Among 3233 AF patients without pre‐existing HF, we investigated 2459 patients with the data of LVEF at enrolment. We divided the patients into three groups stratified by LVEF [mildly reduced LVEF (40–49%), below normal LVEF (50–59%), and normal LVEF (≥60%)] and compared the backgrounds and incidence of HF hospitalization between the groups. Of 2459 patients [mean age: 72.4 ± 10.5 years, female: 917 (37%), paroxysmal AF: 1405 (57%), and mean CHA2DS2‐VASc score: 3.0 ± 1.6], the mean LVEF was 66 ± 8% [mildly reduced LVEF: 114 patients (5%), below normal LVEF: 300 patients (12%), and normal LVEF: 2045 patients (83%)]. Patients with lower LVEF demonstrated lower prevalence of female and paroxysmal AF (both P < 0.01), but age and CHA2DS2‐VASc score were comparable between the three groups (both P > 0.05). During the median follow‐up period of 6.0 years, 255 patients (10%) were hospitalized for HF (annual incidence: 1.9% per person‐year). Multivariable Cox regression analysis demonstrated that lower LVEF strata were independently associated with the risk of HF [mildly reduced LVEF (40–49%): hazard ratio = 2.98, 95% confidence interval = 1.99–4.45 and below normal LVEF (50–59%): hazard ratio = 2.01, 95% confidence interval = 1.44–2.82, compared with normal LVEF (≥60%)] after adjustment by age, sex, type of AF, and CHA2DS2‐VASc score. LVEF < 60% was significantly associated with the higher risk of HF hospitalization across all major subgroups without significant interaction (P for interaction; all P > 0.05). LVEF had an independent and incremental prognostic value for HF hospitalization in addition to natriuretic peptide levels in AF patients without pre‐existing HF.ConclusionsLower LVEF was significantly associated with the higher incidence of HF hospitalization in AF patients without pre‐existing HF, leading to the future risk stratification for and prevention of incident HF in AF patients.
Oxidative DNA damage on the VEGF G‐quadruplex forming promoter is repaired via long‐patch BER
Authors: Hussen Adil S. , Kravitz Haley L. , Freudenthal Bret D. , Whitaker Amy M. 
Published: August 2023

AbstractIn response to oxidative damage, base excision repair (BER) enzymes perturb the structural equilibrium of the VEGF promoter between B‐form and G4 DNA conformations, resulting in epigenetic‐like modifications of gene expression. However, the mechanistic details remain enigmatic, including the activity and coordination of BER enzymes on the damaged G4 promoter. To address this, we investigated the ability of each BER factor to conduct its repair activity on VEGF promoter G4 DNA substrates by employing pre‐steady‐state kinetics assays and in vitro coupled BER assays. OGG1 was able to initiate BER on double‐stranded VEGF promoter G4 DNA substrates. Moreover, pre‐steady‐state kinetics revealed that compared to B‐form DNA, APE1 repair activity on the G4 was decreased ~two‐fold and is the result of slower product release as opposed to inefficient strand cleavage. Interestingly, Pol β performs multiple insertions on G4 substrates via strand displacement DNA synthesis in contrast to a single insertion on B‐form DNA. The multiple insertions inhibit ligation of the Pol β products, and hence BER is not completed on the VEGF G4 promoter substrates through canonical short‐patch BER. Instead, repair requires the long‐patch BER flap‐endonuclease activity of FEN1 in response to the multiple insertions by Pol β prior to ligation. Because the BER proteins and their repair activities are a key part of the VEGF transcriptional enhancement in response to oxidative DNA damage of the G4 VEGF promoter, the new insights reported here on BER activity in the context of this promoter are relevant toward understanding the mechanism of transcriptional regulation.
Metal Mixture Toxicity of Ni, Cu, and Zn in Freshwater Algal Communities and the Correlation of Single-Species Sensitivities Among Single Metals: A Comparative Analysis
Authors: Fettweis Andreas , Hansul Simon , Schamphelaere Karel De , Smolders Erik 
Published: August 2023

Abstract
The effects assessment of metals is mainly based on data of single metals on single species, thereby not accounting for effects of metal mixtures or effects of species interactions. Both of these effects were tested in combination, thereby hypothesizing that the sensitivity of a community to synergistic mixture toxicity depends on the correlation of single-species sensitivities among the single metals. Single-metal and metal-mixture effects were tested in full concentration–response experiments (fixed ray of 1:1:3 and 5:1:13 mass ratio Ni:Cu:Zn) on eight single freshwater algal species and 14 algal communities of four species each. The mean correlation of single-species median effect concentrations among the single metals (Ni–Cu, Cu–Zn, and Zn–Ni) for all species in a community (r̅) ranged from −0.4 to 0.9 among the communities; most of these (12/14) were positive. Functional endpoints (total biomass) were overall less sensitive than structural endpoints (Bray-Curtis similarity index) for communities with positively correlated single-species sensitivities among the single metals (r̅&gt;0.33), suggesting that such correlations indicate functional redundancy under metal-mixture stress. Antagonistic metal-mixture interactions were predominantly found in single species, whereas metal-mixture interactions were antagonistic and surprisingly synergistic for the communities, irrespective of the reference mixture model used (concentration addition or independent action). The mixture interactions close to the carrying capacity (day 7) of communities gradually shifted from antagonism to more noninteractions with increasing correlation of single-species sensitivities among the single metals. Overall, this suggests that functional redundancy under mixed-metal stress comes at the cost of reduced biodiversity and that synergisms can emerge at the community level without any synergisms on the single-species level. Environ Toxicol Chem 2023;42:2666–2683. © 2023 SETAC.
Immunostimulatory effects of marine algae extracts on in vitro antigen‐presenting cell activation and in vivo immune cell recruitment
Authors: Ho Thi Len , Lee Jueun , Ahn So Yeon , Lee Dong‐Ha , Song Woo‐Jin , Kang Inhae , Ko Eun‐Ju 
Published: August 2023

AbstractMarine algae are photosynthetic eukaryotic organisms that are widely used as sources of food, cosmetics, and drugs. However, their biological and immunological effects on immune cells have not been fully elucidated. To unravel their immunological activity and broaden their application, we generated antigen‐presenting cells (APCs), including dendritic cells (DCs) and macrophages, from mouse bone marrow cells and treated them with six different marine algae extracts (MAEs). We evaluated cell viability, activation marker expression, and pro‐inflammatory cytokine production by APCs after 2 days of MAE treatment. All six MAEs significantly induced cytokine production of APCs, among which Pyropia yezoensis (PY), Peyssonnelia caulifera (PC), and Meristotheca papulosa (MP) extracts exhibited the strongest effect. Cladophora wrightiana var. minor (CW) extract moderately upregulated cytokine levels but increased the expression of activation markers on DCs. Moreover, PY, PC, MP, Sargassum pectinifera (SP), and Caulerpa okamurae (CO) pre‐treated APCs effectively stimulated T‐cell proliferation and cytokine production. Furthermore, the mice injected with MAEs exhibited higher cytokine (TNF‐α, IL‐6, and IL‐1β) production as well as enhanced innate immune cell recruitment capacities (DCs, monocytes, neutrophils, and natural killer cells) in the peritoneal cavity of the mice compared to those of the non‐treated mice. Therefore, all MAEs exhibited immunostimulatory potential, with PY, PC, CW, and MP extracts being the most effective in stimulating immune responses and cell activation. To the best of our knowledge, this is the first study to determine the immunomodulatory activities of six MAEs both in vitro and in vivo.
Effects of saline water with different concentrations of calcium and magnesium ions on emitter clogging in drip irrigation integrated water–fertilizer system
Authors: Zhou Long , Wei Chenchen , Yang Peiling , Li Yunkai , Zhangzhong Lili , Su Yanping , Wang Xin , He Xin 
Published: August 2023

AbstractThe application of saline water in drip irrigation is severely constrained due to its high salt content, which causes precipitation and emitter clogging. Therefore, the effects of calcium and magnesium ions at 100, 150 and 200 mg/L in saline water coupled with humic acid nitrogen fertilizer on emitter clogging were studied. Compared with ground saline water, all treatments significantly increased the emitter clogging risk. In the calcium and magnesium ion treatment groups, clogging fouling increased by 23.94–58.35 and 14.55–51.21 mg/cm2, the discharge variation ratio (Dra) decreased by 22%–59% and 14%–49%, and the Christiansen coefficient of uniformity (CU) decreased by 29%–79% and 15%–74%, respectively. Higher ion concentrations caused more clogging. Saline water with a high calcium content caused more clogging. Under similar concentration conditions, the Dra and CU in high magnesium saline water were 9%–32% and 8%–51% higher, respectively. In addition, the increase in magnesium ion concentration changed the crystal form of carbonate, slowed the formation rate of precipitated dirt and significantly reduced the carbonate content by 19%–40%. Our results provide support for the popularization of saline water drip irrigation systems.
Physical therapy provision for patients with pneumonia in US hospitals
Authors: Johnson Joshua K. , Young Daniel L. , Guo Ning , Tereshchenko Larisa G. , Martinez Maylyn , Hohman Jessica A. , Rothberg Michael B. 
Published: August 2023

AbstractBackgroundPhysical therapy (PT) appears beneficial for hospitalized patients. Little is known about PT practice patterns and costs across hospitals.ObjectiveTo examine whether receiving PT is associated with specific patient and hospital characteristics for patients with pneumonia. We also explored the variability in PT service provision and costs between hospitals.MethodsWe included administrative claims from 2010 to 2015 in the Premier Healthcare Database, inclusive of 644 US hospitals. We examined associations between receiving at least one PT visit and patient (age, race, insurance, intensive care utilization, comorbidity status, and length of stay) and hospital (academic status, rurality, size, and location) characteristics. Exploratory measures included timing and proportion of days with PT visits, and per‐visit and per‐admission costs.ResultsOf 768,010 patients, 49% had PT. After adjustment, older age most significantly increased the probability of receiving PT (+38.0% if >80 vs. ≤50 years). Higher comorbidity burden, longer length of stay, and hospitalization in an urban setting were also associated with higher probability. Hospitalization in the South most significantly decreased the probability (−9.1% vs. Midwest). Patients without Medicare and Non‐White patients also had lower probability. Median (interquartile range) days to first visit was 2 (1–4). Mean proportion of days with a visit was 35% ± 20%. Median per‐visit cost was $88.90 [$56.70–$130.90] and per‐admission was $224.00 [$137.80–$369.20].ConclusionBoth clinical (intensive care utilization and comorbidity status) and non‐clinical (age, race, rurality, location) factors were associated with receiving PT. Within and between hospitals, there was high variability in the number and frequency of visits, and costs.