bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-Genome Sequencing and Metagenomics Reveals Diversity and Prevalence of Soil Listeria spp. in the Nantahala National Forest
Authors: Wang Jia , Schamp Claire N. , Hudson Lauren K. , Chaggar Harleen K. , Bryan Daniel W. , Garman Katie N. , Radosevich Mark A. , Denes Thomas G. 
Published: August 2023

Abstract

Background. Listeria spp. are widely distributed environmental bacteria associated with human foodborne illness. Whole-genome sequencing (WGS) of Listeria monocytogenes clinical isolates is the current standard for surveillance and identifying outbreaks. WGS of Listeria isolates can assist in understanding the diversity of Listeria species. The capability of precise detection and characterization of Listeria spp. strains in the natural environment is important for identifying transmission routes that can lead to food contamination and human illness. Recently, metagenomic sequencing has emerged as a promising tool for the direct detection of Listeria spp. in environmental samples. The objective of this study was to use a practical hybrid approach that can provide valuable information on the occurrence of Listeria spp. in soil environments in the southern Appalachian region of the United States.
Results.
This study involved soil sampling from four locations across varying altitudes in the Nantahala National Forest. Forty-two Listeria spp. isolates and 12 metagenomes of soil bacterial communities were obtained. Analysis of the isolates genomes revealed 14 distinct strains, representing a diversity of Listeria species. The predominant species isolated was L. cossartiae subsp. cayugensis (n = 8), with the other species including L. monocytogenes (n = 3), ‘L. swaminathanii’ (n = 1), L. marthii (n = 1), and L. booriae (n = 1). Most distinct strains (n = 13) were isolated from lower altitudes (1,500 or 2,500 ft), while the ‘L. swaminathanii’ strain was isolated from two different altitudes. Metagenomic analysis demonstrated a decline in both bacterial community diversity and relative abundance of Listeria spp. with increasing altitude. Of the soil factors assessed, pH and cation exchange capacity were significantly positively correlated with the abundance of Listeria spp. in soil as detected by metagenomics.
Conclusions.
By integrating culture-independent metagenomics with culture-based WGS, this study provides insights into the diversity and prevalence of Listeria spp. in the Nantahala National Forest. These findings contribute to the current knowledge on the distribution of Listeria spp. in the natural environment and offer a potential culture-independent method for tracking the transmission of foodborne pathogens.
Analysis of the impact of a new successive interference cancellation decoder on outage probability performance in power Domain Non- Orthogonal Multiple Access Transmission
Authors: Talla Belmien Carlos Konlack , Bossou Olivier Videme , Feudjio Cyrille , Mbogue Joseph Ngimbog , Ngoufo Gédéon Ghislain Nkwewo , Tchuidjan Roger 
Published: August 2023

Abstract
In this article, we evaluate the impact of a new successive interference cancellation scheme on the signal outage probability in the context of PD-NOMA (Power Domain Non-Orthogonal Multiple Access) transmissions. The outage probabilities were assessed for 2 users, UE1 (closest to the base station), and UE2 (farthest from the base station), in a Rayleigh fading channel. The conducted simulations enable a comparison of signal outage probabilities for mobiles using a proposed successive interference cancellation (SIC) decoding scheme, where all interferences with other signals are cancelled, and a standard successive interference cancellation scheme, where only interferences from stronger signals are cancelled. Our simulations establish that the proposed SIC decoding model improves the outage probability performance by 25% for the user farthest from the base station. Generally, an improvement is observed for all mobiles in the network except for the strongest user closest to the base station. In fact, the performance of both SIC decoding models remains identical for the specific case of the strongest user.
Software Reliability Growth Modelling Framework to Study SDN Controllers
Authors: Anand Adarsh , Gupta Priyanka , Chhipa Mayur Kumar , Mellal Mohamed Arezki , Johri Prashant 
Published: August 2023

Abstract

SDN, as an emerging centralized network control platform, has been studied widely by many researchers. To make this complex and fine-grained network platform more reliable and secure, the need to understand its fault debugging pattern was observed. The testing phase of the software has undoubtedly been considered as a crucial phase that ensures high quality network architecture to its users. The pre-release testing activities are solely conducted by the testers where the debugging process after its release comprises both testers and users. The study of the joint role of testers and users has been devoid in the field of network systems. This paper methodically models the complete testing profile (before and after release) of the SDN Controller. The two-staged modeling process is utilized to model the detection and resolution of network faults after its release in which the joint debugging activities of testers and users are catered with the help of Stieltjes convolution. The two-stage model addresses the release point transition. The proposal has been validated on two real life failure data sets. The validation shows promising results.
Task-oriented exercise effects on walking and corticospinal excitability in multiple sclerosis: protocol for a randomized controlled trial
Authors: Moslemi Zahra , Toledo-Aldana Eduardo A. , Baldwin Bruce , Donkers Sarah J. , Eng Janice J. , Mondal Prosanta , de Zepetnek Julia O. Totosy , Buttigieg Josef , Levin Michael , Mang Cameron S. 
Published: August 2023

Abstract
Background
Multiple sclerosis (MS) is a degenerative disease of the central nervous system (CNS) that disrupts walking function and results in other debilitating symptoms. This study compares the effects of ‘task-oriented exercise’ against ‘generalized resistance and aerobic exercise’ and a ‘stretching control’ on walking and CNS function in people with MS (PwMS). We hypothesize that task-oriented exercise will enhance walking speed and related neural changes to a greater extent than other exercise approaches.
Methods
This study is a single-blinded, three-arm randomized controlled trial conducted in Saskatchewan, Canada. Eligible participants are those older than 18 years of age with a diagnosis of MS and an expanded Patient-Determined Disease Steps (PDDS) score between 3 (‘gait disability’) and 6 (‘bilateral support’). Exercise interventions are delivered for 12 weeks (3×60-min per week) in-person under the supervision of a qualified exercise professional. Interventions differ in exercise approach, such that task-oriented exercise involves weight-bearing, walking-specific activities, while generalized resistance and aerobic exercise uses seated machine-based resistance training of major upper and lower body muscle groups and recumbent cycling, and the stretching control exercise involves seated flexibility and relaxation activities. Participants are allocated to interventions using blocked randomization that stratifies by PDDS (mild: 3–4; moderate: 5–6) Assessments are conducted at baseline, post-intervention, and at a six-week retention time point. The primary and secondary outcome measures are the Timed 25-Foot Walk Test and corticospinal excitability for the tibialis anterior muscles determined using transcranial magnetic stimulation (TMS), respectively. Tertiary outcomes include assessments of balance, additional TMS measures, blood biomarkers of neural health and inflammation, and measures of cardiorespiratory and musculoskeletal fitness.
Discussion
A paradigm shift in MS healthcare towards the use of “exercise as medicine” was recently proposed to improve outcomes and alleviate the economic burden of MS. Findings will support this shift by informing the development of specialized exercise programming that targets walking and changes in corticospinal excitability in PwMS.
Trial registration
ClinicalTrials.gov, NCT05496881, Registered August 11, 2022
Dissipation of Insecticidal Residues having Different Modes of Action in Cabbage
Authors: Akoijam Romila , Ningombam Arati , Sonia Chongtham , Devi Ch. Premabati , Singh Telem Ratan , Singh I. M. 
Published: August 2023

Abstract
The dissipation study of residues of insecticides having different modes of action was studied in cabbage. The insecticides such as deltamethrin, chlorantraniliprole and fipronil residues were sprayed as foliar application twice at an interval of 7 days with the recommended doses of deltamethrin (@ 12.5 g a.i./ha), chlorantraniliprole (@ 30 g a.i./ha) and fipronil (50 g a.i./ha). The samples were taken randomly before the application of any insecticides and 1 hour after the second spray of insecticides. The residues were below the detectable limit for all the insecticides. The residues of fipronil were found to be more persistent than those of chlorantraniliprole and deltamethrin. Fipronil was found to persist up to the 7th day with a residue of 0.01 mg kg-1. However, the residues of chlorantraniliprole and deltamethrin persisted upto the 5th day i.e. 0.11 mg kg-1 and 0.03 mg kg-1 respectively. Observation revealed that the residues of chlorantraniliprole on cabbage head dissipated over the course of time and an equally high degree of dissipation was observed. The half-life of chlorantraniliprole was found to be 1.81 days as compared to 1.07 and 1.42 days respectively for fipronil and deltamethrin.
Foot biomechanics in patients with advanced subtalar- and Chopart joint osteoarthritis and poorly responding to conservative treatment
Authors: Deschamps Kevin , Mercken Karel , Verschuren Pieter , Eerdekens Maarten , Vanstraelen Eline , Wuite Sander , Matricali Giovanni 
Published: August 2023

Abstract
Background
A comprehensive insight into the effects of subtalar- and Chopart joint osteoarthritis on lower limb’s biomechanical characteristics during walking is lacking. Our goal was to assess joint kinematics and kinetics and compensatory mechanisms in patients with subtalar and Chopart joint osteoarthritis.
Methods
Patients with symptomatic and radiographically confirmed osteoarthritis of the subtalar and Chopart joint (n = 10) and an asymptomatic control group (n = 10) were compared. Foot joint kinematics and kinetics during the stance phase of walking were quantified using a four segment foot model.
Results
During pre-swing phase, the Ankle range of motion in the sagittal plane of the patient group decreased significantly (P = 0.001), whereas the Lisfranc joint range of motion in the sagittal plane was greater in the pre-swing phase (P = 0.003). The Chopart joint showed lower transverse plane range of motion in the patient group during the loading response and pre-swing phase (P < 0.001 resp. P = 0.002). The patient group showed a lower Ankle peak plantarflexion moment (P = 0.004), peak plantarflexion velocity (P < 0.001) and peak power generation in the sagittal plane (P < 0.001), and a lower Chopart joint peak adduction and abduction velocity (P < 0.001 resp. P < 0.001) and peak power absorption (P < 0.001).
Conclusions
These findings suggest that patients with subtalar and Chopart joint osteoarthritis adopt a cautious walking strategy potentially dictated by pain, muscle weakness, kinesiophobia and stiffness. Since this poorly responding population faces surgical intervention on the short term, we recommend careful follow-up after fusion surgery since biomechanical outcome measures associated to this post-surgical stage is lacking.
Comparison of clinical efficacy of Hybrid fixation, single-screw and single-Tightrope in the treatment of ankle fractures complicated with Distal tibiofibular syndesmosis injury
Authors: Xu Jingcheng , Jie Ke , Zhang Kaiting , Li Xue , Zou Yunxuan , Shen Guodong , Zhu Yongzhan 
Published: August 2023

Abstract
Background In the treatment of ankle fracture combined with distal tibiofibular syndesmosis injury, both screw fixation and Tightrope fixation are commonly used to restore the anatomical relationship of distal tibiofibular syndesmosis. In recent years, some studies have reported the use of hybrid fixation (Tightrope combined with screws) for the repairment of distal tibiofibular syndesmosis injury. However, there is a lack of literature reports comparing the clinical efficacy of screw fixation, Tightrope fixation and hybrid fixation.Purpose To explore whether there is any difference in the clinical efficacy of single screw fixation, single Tightrope fixation and hybrid fixation by comparing the imaging manifestations, AOFAS scores and VAS scores.Methods From January 2017 to January 2023, patients with ankle fracture combined with distal tibiofibular syndesmosis injury who were treated with single screw fixation, single Tightrope fixation, or hybrid fixation were retrospectively reviewed. Clinical evaluation included AOFAS score and VAS score. Imaging evaluation included medial malleolar space (MCS), tibiofibular clear space (TFCS) and tibiofibular overlap (TFO). All measurements were performed on anteroposterior ankle radiographs.Results There were 36 patients in single screw fixation group, 30 patients in single Tightrope fixation group and 18 patients in hybrid fixation group. All three groups had statistical similar clinical and imaging results. The hybrid fixation group was further divided into two subgroups: the group with removed screw(n = 8) and the group without removed screw(n = 8). Clinical and imaging outcomes were statistical similar in these two subgroups.Conclusion Comparing with single screw fixation and single Tightrope fixation, the hybrid fixation is comparably effective and has similar clinical efficacy. Considering that the configuration of the hybrid fixation is similar to that of the healthy ankle in terms of biomechanics, it may be helpful for early weight-bearing and can be used as an alternate method for distal tibiofibular syndesmosis fixation.
Prokaryotic microbial diversity analysis and metabolic function prediction of salt lakes on the Qinghai-Tibet Plateau
Authors: Zhang Man , Li Yongzhen , Zhu Derui , Xing Jiangwa , Long Qifu , Shen Guoping 
Published: August 2023

Abstract
The Dong Taijinar Salt Lake (DT) and Xi Taijinar Salt Lake (XT) have been widely studied as mineral-rich areas. However, little is known about the composition and distribution of the microbial communities in these two lakes. In this study, metagenomics sequencing was used to analyze the diversity and potential functions of the microbial communities in DT and XT. According to our report, the salinity of DT (332.18–358.30 g/L) was 10 times higher than that of XT (20.09–36.83 g/L). Interestingly, the dominant domain in DT was Archaea (96.16%), while that in XT was Bacteria (93.09%). The distribution of Bacteria in the DT revealed 33 phyla and 1717 genera. The dominant genus in DT was Marinobacillus, which was positively correlated with total phosphorus content. There were four main phyla and 153 genera identified in the Archaea of DT. The most abundant Archaea genera in DT were Natronomonas (24.61%) and Halorubrum (23.69%), which were mainly positively correlated with the Na+, Ca2+, and Cl− contents. Similarly, there were 33 phyla and 1906 genera of Bacteria in XT, and Loktanella was the dominant genus. The archaeal taxonomy in XT mainly included four phyla and 149 genera. Proteobacteria and Euryarchaeota were the most abundant bacterial and archaeal phyla in the two salt lakes. Analysis of the halophilic mechanisms of the microorganisms identified in these two salt lakes revealed that the Bacteria in XT preferred to synthesize compatible solutes, whereas the Archaea in DT preferred a "salt-in" adaptation strategy in salt-stressed environments.
FUSE binding protein1 interacts with Tumor Suppressor p53 and p53-Isoforms through their DNA Binding domain: Mapping the FBP1 binding site
Authors: Dixit Updesh , Pandey Vaishali , Comollo Thomas W , Upadhyay Alok , Pandey Virendra N 
Published: August 2023

Abstract
We have earlier demonstrated that a cellular factor, FUSE binding protein1 (FBP1), physically interacts and effectively suppresses the function of tumor suppressor p53 and promotes persistent HCV replication [Dixit et al. JVI 89:7905, 2015). In the present study, we demonstrate that FBP1 interacts with various naturally occurring p53-isoforms isolated from different cancers that carry large deletions at the N- and C-terminal regions but still have an intact DNA binding domain (DBD). We discovered that FBP1 specifically interacts with the DNA binding domain (DBD) of p53 and its isoforms. We further mapped the FBP1-interaction site and identified a 21-residue-long motif spanning amino acid residues 163–183 in the p53-DBD. We further confirmed that Arg175/Cys176, within this motif, is necessary for FBP1 interaction. Arg175/Cys176, located at the junction of the β4 and H1 helix of the L2 Loop, is required for the DNA binding function of p53. Occupying this site containing Arg175/Cys176 by FBP1 may block the DNA binding function of p53.
TBTC: A Temporal Knowledge Graph Reasoning Model with Bidirectional Temporal Correlation
Authors: Xie Bohan , Wang Shirui , Ding Ling , Chen Jianting , Xiang Yang 
Published: August 2023

Abstract
With the rapid development of the Internet, data with time information shows an explosive growth. However, the traditional knowledge graphs don’t consider the temporal information of facts, which brings inconvenience to downstream tasks, so people proposed the temporal knowledge graph. The temporal knowledge graph models the timeliness of facts in the form of quadruples, which clearly represents the evolution process of knowledge.But it is difficult for temporal knowledge graphs to cover the full of worldwide knowledge. Therefore, predicting missing facts is necessary, including complete historical knowledge and predict future knowledge. However, the existing reasoning models only considered the one-way information transmission from the front to the back in the time dimension, and didn’t effectively use the temporal characteristics of knowledge. And the treatment of the unseen entities is too rough when predicting the future knowledge. To solve above problems, we proposed a new temporal knowledge graph reasoning model with temporal bidirectional correlation, which models the factual correlation and temporal relationship between entities in a spatial-temporal graph jointly. We also considered the influence of bidirectional information in the time dimension to further enrich the characteristic representation of entities. As for the unseen entities in future knowledge prediction, we proposed a new method to get the embedding of unseen entities, by analyzing its co-occurrence entity set to capture essential common features and generate the embedding vectors. For the above two studies, we conducted experiments on several public datasets, and got better effects.
GOA-Net: Generic Occlusion Aware Networks for Visual Tracking
Authors: Dasari Mohana Murali , Gorthi Rama Krishna 
Published: August 2023

Abstract

Occlusionis a frequent phenomenon that hinders the task of visual object tracking. Since occlusion can be from any object and in any shape, data augmentation techniques will not greatly help identify or mitigate the tracker loss. Some of the existing works deal with occlusion only in an unsupervised manner. This paper proposes a generic deep learning framework for identifying occlusion in a given frame by formulating it as a supervised classification task for the first time. The proposed architecture introduces an ''occlusion classification" branch into supervised trackers. This branch helps in the effective learning of features and also provides occlusion status for each frame. A metric is proposed to measure the performance of trackers under occlusion at frame level. The efficacy of the proposed framework is demonstrated on two supervised tracking paradigms: One is from the most commonly used Siamese region proposal class of trackers, and another from the emerging Transformer-based trackers. This framework is tested on six diverse datasets (GOT-10k, LaSOT, OTB2015, TrackingNet, UAV123, and VOT2018), and it achieved significant improvements in performance over the corresponding baselines while performing on par with the state-of-the-art trackers. The contributions in this work are more generic, as any supervised tracker can easily adopt them.
Platelet-Rich Plasma Injections for Frozen Shoulder: Efficacy in Pain Reduction and Shoulder Function Improvement
Authors: Ahmed Sabih 
Published: August 2023

Abstract
Background: Adhesive capsulitis, commonly known as frozen shoulder, is a condition characterized by stiffness and pain in the shoulder joint. The therapeutic potential of Platelet-rich plasma (PRP) has been increasingly recognized in various orthopedic conditions, yet its specific role in treating frozen shoulder remains underexplored. This study was designed to assess the efficacy of PRP injections in improving outcomes for frozen shoulder patients.
Methods: In this randomized controlled trial, 200 patients diagnosed with frozen shoulder were enlisted. They were evenly allocated into two cohorts: one receiving intra-articular PRP injections and a control group administered with saline injections. Pain intensity was gauged using the Visual Analog Scale (VAS), while shoulder mobility metrics were determined through the Range of Motion (ROM) evaluation. Assessments were conducted at baseline, followed by checks at intervals of 1, 3, and 6 months. Data interpretation employed the t-test and ANOVA.
Results: By the 6-month mark, patients in the PRP group demonstrated a pronounced reduction in VAS scores (average decrement of 4.8) relative to the saline group (average decrement of 1.3). Additionally, the PRP recipients registered substantial enhancements in ROM, particularly in motions of abduction and external rotation, outperforming the control by approximately 60%.
Conclusions:* Our results indicate that PRP injections significantly outpace saline in mitigating pain and enhancing shoulder functionality in frozen shoulder cases. Hence, PRP emerges as a potential primary non-operative treatment for adhesive capsulitis.
Trial registration: This research was duly registered with the **Global Clinical Trial Registry**, bearing the registration identifier **GCTR-2023-02567**, on **January 15, 2023**.