bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urocortin-1 Promotes Colorectal Cancer Cells Migration, Proliferation and Inhibits Apoptosis via Inhibition of p53 Signaling Pathway
Authors: Guo Xiaolan , Li Ya , Chen Xiangyu , Sun Binghua 
Published: August 2023

Abstract
Purpose
To investigate the effect of urocortin-1 (UCN-1) on the growth, migration and apoptosis of colorectal cancer (CRC) in vivo and vitro and mechanism of UCN-1 modulating CRC cells in vitro.
Methods
The correlation between UCN-1 and CRC was evaluated by Cancer Genome Atlas (TCGA) database and the tissues microarray. The expression of UCN-1 in CRC cells was explored by quantitative real-time polymerase chain reaction (RT-qPCR) or western blot. In vitro, the influence of UCN-1 on proliferation, apoptosis and migration HCT-116 and RKO cells were explored by celigo cell counting assay, flow cytometry and wound healing assay or transwell, respectively. In vivo the effect of UCN-1 on CRC tumor growth and progression was evaluated in the nude mice. The downstream pathway behind UCN-1 regulating CRC was found by phospho-kinase profiler array in RKO cells. Expression of UCN-1 in cells was knocked down or upregulated using lentivirus.
Results
Both of the results of TCGA database and the tissues microarray shown that UCN-1 strongly expressed in tissues of CRC patients. Furthermore, the tissues microarray results showed that expression of UCN-1 was higher in male CRC patients than that in female patients, and high expression of UCN-1 was associated with higher risk of lymphatic metastasis and later pathological stage. Additionally, knockdown of UCN-1 in CRC cells caused a reduction in cell proliferation, migration, and colony formation as well as an increase in apoptosis. In xenograft experiments, tumors generated from RKO cells with UCN-1 knockdown exhibited declined tumor volume and weight. Reduction of the expression of Ki67 in xenograft tumors reflected that knockdown of UCN-1 curbed the growth of CRC tumors. Furthermore, the human phospho-kinase array showed that p53 signal pathway participated in UCN-1-mediated CRC development. The suppression in migration and proliferation caused by UCN-1 knockdown was reversed by inhibitors of p53 signal pathway, while the increase of cell apoptosis was withdrawn. On the other hand, overexpression of UCN-1 promoted the proliferation and migration and inhibited apoptosis of CRC cells. Overexpression of p53 reversed the effect of UCN-1 overexpression on CRC development.
Conclusion
UCN-1 promotes the migration, proliferation and inhibits apoptosis via inhibition of p53 signaling pathways.
Differentiation between depression and bipolar disorder in child and adolescents by voice features
Authors: luo jie , wu yuanzhen , Liu Mengqi , Li Zhaojun , Wang Zhuo , zheng yi , Feng Lihui , lu jihua , he fan 
Published: August 2023

Abstract
Objective: Major depressive disorder (MDD) and bipolar disorder (BD) are serious chronic disabling mental and emotional disorders, with symptoms that often manifest atypically in children and adolescents, making diagnosis difficult without objective physiological indicators. Therefore, we aimed to objectively identify MDD and BD in children and adolescents by exploring their voiceprint features.
Methods:This study included a total of 150 participants, with 50 MDD patients, 50 BD patients, and 50 healthy controls aged between 6 to 16 years. After collecting voiceprint data, chi-square test was used to screen and extract voiceprint features specific to emotional disorders in children and adolescents. Then, selected characteristic voiceprint features were used to establish training and testing datasets with the ratio of 7:3. The performance of various machine learning algorithms was compared using the training dataset, and the optimal algorithm was used to classify the testing dataset and calculate the sensitivity, specificity, accuracy, and ROC curve.
Results: The three groups showed differences in clustering centers for various voice features such as root mean square energy, power spectral slope, low-frequency percentile energy level, high-frequency spectral slope, spectral harmonic gain, and audio signal energy level. The model of linear SVM showed the best performance in the training dataset, achieving a total accuracy of 95.6% in classifying the three groups in the testing dataset, with sensitivity of 93.3% for MDD, 100% for BD, specificity of 93.3%, AUC of 1 for BD, and AUC of 0.967 for MDD.
Conclusion: By exploring the characteristics of voice features in children and adolescents, machine learning can effectively differentiate between MDD and BD in a population, and voice features hold promise as an objective physiological indicator for the auxiliary diagnosis of mood disorder in clinical practice.
Federated Learning for Collaborative Network Security in Decentralized Environments
Authors: Neyigapula Bheema Shanker 
Published: August 2023

Abstract
In decentralized network environments, collaborative efforts are crucial to bol- stering network security against ever-evolving threats from malicious actors. Federated Learning has emerged as a promising solution, enabling multiple nodes to collectively train machine learning models while preserving data privacy. This research proposes SentinelNet, a novel Federated Learning framework specif- ically designed for collaborative network security. The framework emphasizes secure threat intelligence sharing, privacy-preserving techniques, and adaptive learning mechanisms. Through comprehensive evaluations and real-world case studies, SentinelNet demonstrates its efficacy in enhancing network security while maintaining data confidentiality. The research highlights the significance of col- laborative approaches and advocates the adoption of Federated Learning to fortify decentralized network ecosystems.
Asiaticoside reduces tumor angiogenesis by inhibiting prostate cancer stem cells in an acidic microenvironment
Authors: ZHONG Shaowen , Chen Zhihao , GUO Qijie , HUANG Sheng , XIE Shangyan , HUANG Shuai , WANG Bin 
Published: August 2023

Abstract
Prostate cancer (PCa) is one of the most common malignant tumors in men worldwide. The tumor microenvironment (TME) plays an important role in the development of tumors, and regulation of the acidic TME caused by insufficient tissue perfusion and tumor progression is an important strategy in cancer treatment. However, the effects of an acidic TME on PCa are unknown. In this study, we first examined the effects of an acidic TME on PCa through bioinformatics analysis of the TCGA database, and proposed a prognostic risk assessment model based on the acidic TME that could effectively predict the progression, metastasis and prognosis of PCa. Then, using cell proliferation, matrix adhesion, and Transwell migration and invasion assays, we showed that the acidic TME promoted PCa development and progression. Finally, we demonstrated that Asiaticoside, a traditional Chinese herb, may act as a potential drug to target and inhibit cancer stem cells (CSCs), thereby reducing tumor angiogenesis in PCa. Taken together, this study provided new perspective for the pathogenesis of PCa and highlighted a novel therapeutic strategy that targets PCa CSCs.
Preparation and characterization of poly(lactic acid) (PLA) based poly(ethylene glycol)/eugenia caryophyllus essential oil added smart composite nanofibers for biothermal stabilization
Authors: Ertuğral Tuğba Güngör 
Published: August 2023

Abstract
Poly(ethylene glycol) (PEG) polymer is useful as phase change material (PCM) in thermal energy storage systems. In this study, PEG 1000 and clove essential oil (EO) blended with various mass ratios (100/100/25, 150/100/25, 200/100/25) PEG/PLA/EO nanofibers were produced by electrospinning in a poly(lactic acid) (PLA) polymer matrix. The produced nanofibers were characterized by scanning electronic microscopy (SEM), fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The fibers were about 370°C and prepared nanofibers have a phase enthalpy of 77.48 Jg-1 at 34.74°C. Nanofibers shows promising potential for use in medical, food safety and textile fields.
Surgical Outcomes of Smile Incision and Reverse Shotgun Technique for Distal Interphalangeal Joint Arthrodesis
Authors: Chiang Yi-Sheng , Hsu Cheng-En , Shaw Leo , Chang Chai-Yu , Wang Wei-Chih , Chiu Yung-Cheng 
Published: August 2023

Abstract
Background
Arthrodesis serves as the traditional therapeutic approach for advanced distal interphalangeal joint (DIPJ) arthritis. However, the conventional technique may prove insufficient when the excision of pronounced volar and lateral spurs is required. To address this, we innovated the 'smile incision with reverse shotgun approach'. This method enhances joint exposure and yields superior cosmetic results by extending the transverse skin incision over the DIPJ and cleaving the accessory collateral ligament, thereby improving access to the volar and lateral joint margins. This article meticulously elucidates the surgical procedure and presents preliminary results of its implementation.
Method
The clinical data of 22 consecutive patients (36 fingers) who received DIPJ arthrodesis by this procedure during March 2018 to October 2022, with a mean follow-up period of 9.8 months, were reviewed. Patients’ demographics, union rate, complications, radiographic findings, as well as visual analogue scale (VAS) for pain and satisfaction, were collected and analyzed.
Results
Thirty-five of 36 fingers achieved uneventful bony union (97.2%). The average VAS for pain and satisfaction as well as he coronal plane deviation of DIPJ significantly improved after the operation (all, P < 0.05). No skin necrosis, nail deformity, or infection were observed during the follow-up period.
Conclusion
The smile incision and reverse shotgun approach provided excellent DIPJ exposure, high union rate, and cosmetic appearance for DIPJ arthrodesis surgery. This technique may be a good surgical option for DIPJ arthrodesis when more volar part joint preparation and more volar implant insertion sites are necessary.
Unveiling the emergence of topological quantum phase transitions in the bidirectional transport of carriers through helical edge states in bismuth selenide
Authors: Ukpong Aniekan , Hussien Musa , de Souza Mariano , Tsamparlis Michael 
Published: August 2023

Abstract
Dynamically induced nontrivial band topology in the electronic structure of materials is increasingly being utilized as a primary resource for developing the quantum advantage in emerging technologies. This makes it a fundamental imperative in contemporary condensed matter physics to obtain a deep understanding of the emergence of topological phases during carrier transport in topological matter. In this paper, we have employed a combination of theoretical and computational methods to investigate the emergence of topological quantum transport phases in bismuth selenide and its bias-dependent characteristics by implementing a scalable numerical renormalization group strategy for the carrier transport state. We unravel the emergence of topological quantum phase transitions for carriers hosted on the (001) surface of bismuth selenide because of lattice sublattice asymmetry and spin-orbit coupling and show how the tunnelling transport through the helical surface state is protected against symmetry-breaking perturbations. Our key findings are as follows: (i) charge carriers in bismuth selenide flow bidirectionally through the helical edge states, (ii) the ballistic transport phase undergoes a topological to trivial dynamical phase transition when time reversal symmetry is broken due to an application of a phenomenological field, which may be realized experimentally by impurity doping with ferromagnetic species (iii) quasiparticle interference mediates a transition between different topological quantum phases. These insights are crucial in the rational design of materials for use as interconnects in miniaturized circuits, and manipulation protocols for realizing spontaneous carrier conduction channels using the topological edge states in devices for energy-efficient and lossless transport in microelectronic applications.
Comprehensive analysis of crucial m6A-related differentially expressed genes associated with psoriasis
Authors: Gan Lu , Wu Xuejingzi , Song Jiquan 
Published: August 2023

Abstract
Psoriasis is a chronic inflammatory skin disease that involves genetic and epigenetic factors. N6-methyladenosine methylation (m6A) is the most prevalent RNA modification implicated in various diseases; however, its role in psoriasis remains unclear. Three psoriasis-related datasets were collected from the Gene Expression Omnibus (GEO) database. Differentially m6A methylated genes (DMGs) between psoriasis and controls were identified from the GSE155702 dataset, and corresponding GO and KEGG pathway analyses were performed. Differentially expressed genes (DEGs) and the common DEGs between these two groups were screened from the GSE109248 and GSE142582 datasets. The gene expression of m6A methylation regulators and the interactions among these regulators were also analyzed. 66 significantly upregulated and 381 significantly downregulated m6A peaks were identified corresponding to 414 genes, which were particularly associated with cell and tissue development processes and cell cycle-related items. 271 common DEGs were identified, associating with keratinocyte differentiation, epidermis development, cytokine-cytokine receptor interaction, and fatty acid metabolic processes. 15 crucial m6A-related differentially expressed genes were obtained after the intersection of the DMGs and common DEGs, including NEU2, GALNT6, MTCL1, DOC2B, CAMK2N1, SNTB1, RNF150, CGNL1, CCDC102A, MEOX2, EEF2K, OBSCN, SLC46A2, CCDC85A and DACH1. Our research revealed that psoriasis pathophysiological processes encompass m6A epigenetic alterations, and that m6A alterations may specifically influence cell proliferation and neural regulation and closely associated with osteoarticular involvement and metabolic syndrome in psoriasis.
Identification of NK cell inhibitory ligand CLEC2D-associating immune infiltration landscape and prognostic value in kidney renal clear cell carcinoma
Authors: Chen Cheng , Han Lan , Song Xiujun , Mou Yanhua 
Published: August 2023

Abstract
Background
Kidney renal clear cell carcinoma (KIRC), the most common pathological subtype of RCC, is a characteristics of heterogeneous diseases, and the outcome was dismay with immense diversity. KIRC is obviously resistant to radiotherapy and chemotherapy. In addition, increasing evidence has demonstrated that C-type lectin-like domain family 2 (CLEC2D) expression in cancer contributes to immune escape and thereby emerges as a target for natural killer (NK) cell-mediated immunotherapy. Therefore, we aimed to identify and validate the specific and detailed effect and mechanism of CLEC2D in KIRC.
Methods
CLEC2D expression in KIRC and normal tissues from TCGA were preliminatively screened, and further confirmed in a separate cohort of the GEO. Multivariate analysis and Kaplan-Meier (KM) curves were used to evaluate the relationship between CLEC2D expression and clinicopathologic parameters, and overall survival (OS). Subsequently, noncoding RNAs (ncRNAs) responsible for CLEC2D over-expression were identified by a combination of a series of expression, correlation, and survival analyses. Moreover, we further studied the relationship between CLEC2D expression and immune cell. The compounds were collected from the CellMiner database to examine the significance of CLEC2D in predicting chemotherapy sensitivity.
Results
Contrast to normal tissues, CLEC2D level was significantly higher in KIRC (p < 0.05). Elevated CLEC2D level was tightly correlated with higher grade malignancy and M, N, and tumor stage. Furthermore, univariate and multivariate analyses demonstrated that KIRC cases with high CLEC2D level had shorter OS time than patients with low CLEC2D level (p < 0.05). Subsequently, the LINC00894/-miR-30c-2-3p axis may be considered as the most latent upstream ncRNA-related pathway for CLEC2D in KIRC. Moreover, CLEC2D expression was markedly positively associated with tumor infiltrating immune cells (TIICs), markers of immune cells, and IC. In addition, our research indicated that several drugs IC50s closely related to CLEC2D expression were screened.
Conclusions
In conclusion, abundant expression of CLEC2D was correlated with disease progression and reduced prognosis, and there is sufficient TIICs in KIRC, suggesting that CLEC2D may serve as a promising prognostic biomarker and provide a novel target for tumor immunotherapy of KIRC.
Short-Term Effects of Opioid Use During Therapeutic Hypothermia for Neonatal Encephalopathy
Authors: Jumani Tina , Mishra Priya , Robinson Tonya , Shenberger Jeffrey , Davis Jonathan , Sweigart Benjamin , Turcu Rodica 
Published: August 2023

Abstract
Objective
To examine the effects of opioids during therapeutic hypothermia (TH) on short-term clinical outcomes in neonates with neonatal encephalopathy (NE).
Methods
Multicenter retrospective study of neonates with moderate/severe NE. Opioid exposure was classified as positive (> 0.1 mg/kg) or negative (no exposure or ≤ 0.1 mg/kg) based on cumulative morphine milligram equivalents (MME). Negative binomial regression models were used to evaluate clinical outcomes.
Results
One hundred and twenty neonates were included. Positive opioid exposure was associated with an increase in: 1) length of hospitalization, 2) hypotension/use of vasopressors, and 3) need for and longer duration of mechanical ventilation. Findings were seen with morphine and fentanyl (continuous infusion or intermittent bolus) and were worse with concurrent administration of antiepileptics.
Conclusions
Opioid use during TH was associated with adverse effects on short term clinical outcomes. Caution should be exercised when using opioids during TH until longer-term neurodevelopmental outcome studies can be examined in larger cohorts.
Investigation of Clock Gene Variants in Nannospalax Species in Turkey
Authors: Civelek İlkay , Kankılıç Teoman , Akın Dilara Fatma 
Published: August 2023

Abstract
Blind mole rats (Nannospalax) have become famous for their long lifespans and cancer resistance, but it is still unknown what causes them to be resistant to cancer. These animals may have a different melatonin synthesis mechanism from other creatures due to their lifestyle, which is estimated to be connected to their cancer resistance. In this study, gene variants in Clock genes Cry1, Cry2, Bmal1, Per1 and Per2, which are involved in the synthesis of melatonin in Nannospalax species living in Turkey were investigated and compared with the other organisms including Nannospalax galili, Mus musculus, Heterocephalus glaber, Rattus norvegicus, and Homo sapiens. In addition, it was investigated whether the detected variations have a pathogenic effect in humans. For this purpose, in-vitro methods and some bioinformatics tools were employed in this DNA-based research. 29 variants in total; 11 in Per1, 7 in Per2, 2 in Cry1 and 9 in Cry2 gene, were identified, no variation was found in Bmal1 gene. Some of these variations have been found in regions where DNA repair processes for light-induced UV damage occurred. Other variations were detected in PAS domain and 5'-UTR regions, and the remaining variations were in nondomain regions of proteins. Since Clock gene variations has not been investigated in Nannospalax species, these in-vitro and in-silico methods may give suggestions to choose and focus on detected variations to be used for further studies.
Strengthening of existing pre-engineered building for additional solar panel load using galvanized iron plates
Authors: Kamble Chetana. R. , Patil Rahul. D. , Kumbhar Popat. D. , Desai Piyush. P. 
Published: August 2023

Abstract
In this paper, analysis, and design of an existing PEB installed with solar panels on its roof is performed using STAAD Pro for determining bending stresses to check the structural safety under the action of combined loads viz. dead load, live load, and wind load with additional solar panel load (SL). Failed members due to combined loads with additional SL are identified after the analysis and design by software. To strengthen failed members, analysis and design of PEB were again performed considering galvanized iron plates (GIP) provisions at specified locations. Three different GIP patterns (arrangements) for columns viz. one GIP on one side flange (PC1), one GIP on each flange (PC2), and two GIP at each meeting place of flange and web (PC3) are considered. Similarly, two different GIP patterns for rafters viz. one GIP on bottom flange (PR1) and two GIP at each meeting place of flange and web (PR2) are considered. Results indicate that the bending stresses in the members of PEB with GIP patterns significantly reduced by 56.74% when compared with bending stresses in the members of PEB without GIP patterns. Further, the bending stresses in the columns and rafters of PEB with PC1 and PR1 are found to be lesser by 6.36% and 3.98% respectively when compared with the average bending stresses in the columns and rafters provided with other GIP patterns. The study concludes that the PEB subjected to combined loads with additional SL performs better when its members are strengthened by GIP patterns.
ATP1A1 is a promising new target for melanoma treatment and can be inhibited by its physiological ligand bufalin to restore targeted therapy efficacy
Authors: Soumoy Laura , Genbauffe Aline , Mouchart Lena , Sperone Alexandra , Trelcat Anne , Mukeba-Harchies Léa , Wells Mathilde , Blankert Bertrand , Najem Ahmad , Ghanem Ghanem , Saussez Sven , Journe Fabrice 
Published: August 2023

Abstract
Despite advancements in treating metastatic melanoma, many patients exhibit resistance to targeted therapies. Our study focuses on ATP1A1, a sodium pump subunit associated with cancer development. We aimed to assess ATP1A1 prognostic value in melanoma patients and examine the impact of its ligand, bufalin, on melanoma cell lines in vitro and in vivo. High ATP1A1 expression (IHC) correlated with reduced overall survival in melanoma patients. Resistance to BRAF inhibitor was linked to elevated ATP1A1 levels in patient biopsies (IHC, qPCR) and cell lines (Western blot, qPCR). Additionally, high ATP1A1 mRNA expression positively correlated with differentiation/pigmentation markers based on data from The Cancer Genome Atlas (TCGA) databases and Verfaillie proliferative gene signature analysis. Bufalin specifically targeted ATP1A1 in caveolae (, proximity ligation assay) and influenced Src phosphorylation (Western blot), thereby disrupting multiple signaling pathways (phosphokinase array). In vitro, bufalin induced apoptosis in melanoma cell lines by acting on ATP1A1 (siRNA experiments) and, in vivo, significantly impeded melanoma growth using a nude mouse xenograft model with continuous bufalin delivery via an osmotic pump. In conclusion, our study demonstrates that ATP1A1 could serve as a prognostic marker for patient survival and a predictive marker for response to BRAF inhibitor therapy. By targeting ATP1A1, bufalin inhibited cell proliferation, induced apoptosis in vitro, and effectively suppressed tumor development in mice. Thus, our findings strongly support ATP1A1 as a promising therapeutic target, with bufalin as a potential agent to disrupt its tumor-promoting activity.