bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance and emission characteristics analysis of a CI engine fueled with Distilled pyrolytic waste plastic oil /ethanol/diesel blends
Authors: DAS AMAR KUMAR , Behera Shibaprasad , Mohanty Swopneswar , Behera Nilakantha , Mohanty Kirtiswarup 
Published: September 2023

Abstract
To meet the current demand of energy for agriculture, industry and transportation, the prevailing liquid petro fuels are found insufficient due to their higher depletion rate and inflation in international market. Existing environmental pollution due to higher fossil fuel consumption certainly bring attention of many researchers to identify a better alternative fuel with respect to engine efficiency and exhaust emission. Waste plastic oil (WPO) derived by thermo-catalytic pyrolysis is found a promising alternative fuel due to its similar fuel properties to diesel. WPO contains bulky long chained naphtha, which can be eliminated by fractional distillation resulting production of Distilled Waste Plastic oil (DPO). DPO is mixed with different proportion of ethanol in order to improve the combustion for better performances and lesser emissions. The current study focused on the preparation of homogenous fuel mixtures (DPO/Ethanol/Diesel) to evaluate its engine efficiency and exhaust emissions as compared to pure diesel and confirm that it owns the potential as alternate fuel to CI engine. Test engine trials were performed for determining potential engine characteristics for instance thermal efficiency, specific fuel consumptions and exhaust temperature by using various fuel mixtures (80D10DPO10E, 70D15DPO15E, 60D20DPO20E, 50D25DPO25E) under different loading conditions of test engine. Major pollutants including unburned hydrocarbon, carbon monoxide, and nitrogen oxides were measured by standard emission analyzer. The study concluded that fuel mixture of 60D20DPO20E showed best engine performance and reduced emissions as compared to diesel.
Triggered contraction of self-assembled, micron-scale DNA nanotube rings
Authors: Göpfrich Kerstin , Illig Maja , Jahnke Kevin , Weise Lukas , Scheffold Marlene , Mersdorf Ulrike , Drechsler Hauke , Kierfeld Jan , Diez Stefan 
Published: September 2023

Abstract
Contractile rings formed from cytoskeletal filaments mediate the division of cells. Ring formation is induced by specific crosslinkers, while contraction is typically associated with motor protein activity. Here, we engineer DNA nanotubes as mimics of cytoskeletal filaments and a synthetic crosslinker based on a peptide-functionalized starPEG construct. The crosslinker induces bundling of ten to hundred individual DNA nanotubes. Importantly, the DNA nanotube bundles curve into closed micron-scale rings in a one-pot self-assembly process yielding several thousand rings per microliter. Coarse-grained molecular dynamics simulations reproduce detailed architectural properties of the DNA rings as observed by electron microscopy. Furthermore, theory and simulations predict DNA ring contraction -- without motor proteins -- upon increasing attraction or decreasing bending rigidity of the DNA nanotubes, yielding mechanistic insights into the parameter space relevant for efficient nanotube sliding. We experimentally realize these two conditions by addition of molecular crowders or temperature increase, respectively. In agreement between simulation and experiment, we obtain ring contraction to less than half of the initial ring diameter. DNA-based contractile rings could be a future element of an artificial division machinery in synthetic cells or of contractile muscle-like materials.
MCL1 Inhibition: A Promising Approach to Augment the Efficacy of Sorafenib in NSCLC through Ferroptosis Induction
Authors: Hong Shiao-Ya , Huang Chao-Yuan , Chen Li-Ju , Chen Chi-Shuo , Wang Cheng-Yi 
Published: September 2023

Abstract
Ferroptosis, an iron-dependent form of regulated cell death, plays a crucial role in modulating the therapeutic response in non-small cell lung cancer (NSCLC) patients. Studies have identified the signal transducer and activator of transcription 3 (STAT3) and myeloid cell leukemia-1 (MCL1) as potential targets for sorafenib, which exhibits activities in inducing ferroptosis. However, the role of STAT3-MCL1 axis in sorafenib-induced ferroptosis in NSCLC is still unclear. This study provided evidence that ferroptosis is a critical driver of sorafenib-induced cell death in NSCLC, supported by the accumulation of lipid peroxidation products, indicative of oxidative stress-induced cell death. Additionally, both in vitro and in vivo experiments showed that ferroptosis contributed to a significant portion of the anti-cancer effects elicited by sorafenib in NSCLC. The noticeable accumulation of lipid peroxidation products in sorafenib-treated mice underscored the significance of ferroptosis as a contributing factor to the therapeutic response of sorafenib in NSCLC. Furthermore, we identified the involvement of the STAT3/MCL1 axis in sorafenib-induced antitumor activity in NSCLC. Mechanistically, sorafenib inhibited endogenous STAT3 activation and downregulated MCL1 protein expression, consequently unleashing the ferroptosis driver BECN1 from the BECN1-MCL1 complex. Conversely, there is an augmented association of BECN1 with the catalytic subunit of system Xc−, SLC7A11, whose activity to import cystine and alleviate lipid peroxidation is hindered upon its binding with BECN1. Notably, we found that MCL1 upregulation correlated with ferroptosis resistance in NSCLC upon sorafenib treatment. Our findings highlight the importance of sorafenib-triggered ferroptosis in NSCLC and offer a novel strategy to treat advanced NSCLC patients: by downregulating MCL1 and, in turn, predispose NSCLC cells to ferroptosis.
Occurrence of microplastics in influent, sewage sludge and effluent of municipal wastewater treatment plant, A case study center of Iran, Qom city
Authors: Alasvand Shokoufeh , Asadi-Ghalhari Mahdi , Dehbandi Reza , Ghaffari Mohammad-Ebrahim , Ghadami Mahshid , Ansari Reza , ranjdoost fatemeh 
Published: September 2023

Abstract
The presence of Microplastics (MPs) in the environment is a serious threat to the health of humans and ecosystems. One common source of environmental MPs pollution is the sludge and effluent discharged by wastewater treatment facilities. Typically, there are no specific measures to remove MPs in treatment plants. The purpose of this study is to investigate the presence of MPs in the inlet wastewater, outlet effluent, and sludge of one of the wastewater treatment plants of Qom city in Iran. Monthly sampling was done in the spring and summer of 2022. A total of 18 samples were obtained from wastewater, effluent, and sludge. MPs were identified and separated according to valid guidelines. MPs were subjected to SEM and µ-Raman analysis for further investigation. The average MPs in wastewater and effluent were 710 ± 34.67 and 51 ± 4.42 (MPs/L), respectively, while it was 30.76×103 (MPs/kg) in sludge. The average removal efficiency of MPs in the treatment plant was 97.6%. Overall, polyethylene terephthalate and fibers were the most frequent in the samples. Dominant sizes of MPs in wastewater and sludge were 250–500 µm, while their size in effluent was 250 − 100 µm. Considering the results, it is estimated that 2652×106 MPs enter the environment through effluent every day and can lead to air, soil, and surface water pollution. It makes the need for decisions at the international level to reduce the production and consumption of plastics more necessary.
Vaccine-induced antigen archiving enhances local memory CD8+ T cell responses following an unrelated viral infection
Authors: Tamburini Beth , Doan Thu , Forward Tadg , Lucas Erin , Fleming Ira , Uecker-Martin Aspen , Hesselberth Jay , Morrison Thomas 
Published: September 2023

Abstract
Viral and vaccine antigens persist or are archived in lymph node stromal cells (LNSC) such as lymphatic endothelial cells (LEC) and fibroblastic reticular cells (FRC). Here, we find that, during the time frame of antigen archiving, LEC apoptosis caused by a second, but unrelated, innate immune stimulus such as vaccina viral infection or CpG DNA administration boosted memory CD8+ T cells specific to the archived antigen. In contrast to ”bystander” activation associated with unrelated infections, the memory CD8+ T cells specific to the vaccine archived antigen were significantly higher than memory CD8+ T cells of a different antigen specificity. Finally, the boosted memory CD8+ T cells resulted in increased protection against Listeria monocytogenes expressing the vaccine antigen, but only for the duration that the vaccine antigen was archived. These findings outline a novel mechanism by which LNSC archived antigens, in addition to bystander activation, can augment memory CD8+ T cell responses during repeated inflammatory insults.
Prevalence and predictors of work-related musculoskeletal disorders among healthcare professionals in Sub-Saharan African region: systematic review and meta-analysis protocol
Authors: Aremu Abdulmujeeb Babatunde , Afolabi Ismail Bamidele , Odongo Owino Alfred 
Published: September 2023

Abstract
Background: The Global Burden of Diseases 2017 found that musculoskeletal disorders (MSDs) are the second most prevalent cause of years lost to injury, although years of life lost are decreasing in low-income countries, especially in Sub-Saharan Africa. The objective will be to describe the regional prevalence of WMSD for different anatomical body areas and their risk factors in different health professions in the sub-Saharan African region.
Method: We will search databases such as Scopus, PubMed, Science Direct, AJOL, and Google Scholar for publications published between January 2002 and December 2022. The primary outcome will be the prevalence of work-related musculoskeletal disorders among health professionals, and risk factors related to WRMSDs will be the secondary outcome. Three reviewers will screen all abstract data, full-text articles, and all citations independently. The Newcastle-Ottawa scale (NOS) will be used to assess the quality of eligible publications. Subgroup analysis will be conducted to explore the potential heterogeneity (e.g., age, sample size, gender, and occupational activities). Publication bias and heterogeneity will be assessed and reported using the appropriate tools.
Discussion: This systematic review and meta-analysis will provide a synthesis of the literature on work-related musculoskeletal disorders and their predictors among health professionals in the Sub-Saharan Africa region. The consensus of data from this review will provide a regional view to help occupational health-related policymakers, healthcare professionals, and program managers in developing countries gain a better understanding of the prevalence, causes, and trends to build better evidence-based occupational musculoskeletal health and disorders prevention programs among various health professionals.
Systematic review registration: PROSPERO, CRD42023455517
Impact of COVID-19 pandemic on the maintenance of oral health of the children with special healthcare needs: A cross sectional survey
Authors: Thakur Kajol , Naik Shilpa S , Shetty Ashveeta , Anand Rachna , Balasubramanian Nikitha , Choudhury Shagorika 
Published: September 2023

Abstract
Background
Understanding oral health needs and impediments on children with special healthcare needs (CSHCN) is critical for reducing the oral disease burden, especially after the COVID-19 pandemic.
Aim
To assess the impact of the COVID 19 pandemic on the maintenance of oral health and hygiene of children with special healthcare needs.
Method
This cross-sectional study was conducted on 153 parents of children with special healthcare needs. It was administered using a self-developed questionnaire through the Google Forms; Google LLC, Mountain View, CA with a total of 27 questions about the experience, attitude and challenges faced for providing dental care to their children during the pandemic. Comparison of frequencies of categories of variables with groups was done using chi square test.
Results
Two-thirds of the total respondents (66.0%) reported that their child’s toothbrushing frequency was the same as before the pandemic. 25.5% parents reported a significant surge in their child’s sweet intake. Financial constraint was the cause of negligence in seeking dental care among 70.2% of parents. The greatest discomfort at the dental clinic was the Dentist's attire in among 67.9% of respondents. 48.4% of parents were afraid of their child contracting the virus in the dental operatory. Merely 14.4% of the parents were aware of the concept of Teledentistry.
Conclusion
The findings in our research suggested that although the pandemic had a low impact on the ability of parents to supervise oral healthcare of CSHCN, there is a need for preventive treatments and regular follow ups to prevent exacerbated detrimental effects on their oral health in such future unprecedented events.
Trial Registration:
This cross-sectional questionnaire-based survey was caried out from 1st December 2021 to 30th November 2022 with the approval of the Institutional Ethical Committee. (IREB/2021/PEDO/13).
Comparison of different anastomosis methods in laparoscopically assisted left hemicolectomy
Authors: Li Fan , Xie Yi-Lin , Xu Dong , Lu Chuan-Hui , Guan Guo-Xian 
Published: September 2023

Abstract
Background: This study aims to compare the clinical efficacy of laparoscopically assisted left hemicolectomy with end-to-side anastomosis (ESA) and side-to-side anastomosis (SSA).
Methods: A total of 105 patients from the First Affiliated Hospital of Xiamen University and 229 patients from the First Affiliated Hospital of Fujian Medical University were included in this study, conducted from January 2012 to May 2020. The patients were divided into two groups: 146 cases in the ESA group and 188 cases in the SSA group. Clinical data from both groups were compared, and the survival prognosis was followed up.
Results: The operation time for the ESA group was significantly shorter than that of the SSA group (197.1±57.7 vs 218.6±67.5, c²=4.298, P=0.039). However, no statistically significant differences were observed in intraoperative blood loss, pain score at 48 hours post-operation, first exhaust time, number of dissected lymph nodes, anastomotic leakage, anastomotic stenosis, anastomotic bleeding, and adhesive intestinal obstruction at 6 months, 12 months, and 24 months (P>0.05). Furthermore, there was no significant difference in the 5-year disease-free survival between the two groups (66.4% vs 63.9%, c²=0.156, P=0.693).
Conclusion: Laparoscopically assisted left hemicolectomy with either ESA or SSA for digestive tract reconstruction is feasible. When the tumor is located at the descending colon or the junction of the descending-sigmoid colon, ESA may reduce the need for splenic flexure dissociation during the operation.
Development of PEG-mediated Genetic Transformation and Gene Editing System of Bryum argenteum as a Abiotic Stress Tolerance Model Plant
Authors: Leng Fengjun , Zhou Guiwei , Shi Ruoyuan , Liu Chengyang , Lin Yirui , Yu Xinqiang , Zhang Yanhua , He Xiangxi , Liu Zhu , Bao Fang , Hu Yong , He Yikun 
Published: September 2023

Abstract
Bryum argenteumis a fascinating, cosmopolitan, and versatile moss species that thrives in various disturbed environments. Because of its comprehensive tolerance to the desiccation, high UV and extreme temperatures, so it is emerging as a model moss for studying the molecular mechanisms underlying the responses of plants to abiotic stresses. However, due to the lack of basic tools such as gene transformation, targeted genome modification, molecular mechanisms underlying the survival of B. argenteum in different environments are poorly understood. Here, we reported the protonema of B. argenteum can survive up to 95.42% water loss. The genome size of B. argenteum is approximately 313 Mb by kmer analysis, smaller than reported 700 Mb. Then, we established a simple method for protonema induction and an efficient protoplast isolation and regeneration protocol for B. argenteum. Moreover, a PEG-mediated B. argenteum protoplast transient transfection and stable transformation system was developed. Two homologues of ABI3(ABA-INSENSITIVE 3) gene were cloned from B. argenteum. This genetic transformation system was further optimized for CRISPR/Cas9 system for targeted delivery of the BaABI3A and BaABI3B gene into B. argenteum protoplasts, which triggered mutagenesis at the target in about 2%-5% of the regenerated plants. Isolated ABI3A and ABI3B mutants are all sensitive to desiccation, which suggested BaABI3A and BaABI3B play redundant roles in desiccation stress. Our results provide a rapid and simple approach for molecular genetics in B. argenteum. The results of this study will help to understand the molecular mechanisms of plant extreme environmental adaptation.
Investigation of the main regularities of glycolysis of polycarbonate plastic during catalysis by basic agents
Authors: Kurneshova Tatyana A. , Dzhabarov Georgy V. , Sapunov Valentin N. , Kozlovskiy Roman A. , Voronov Mikhail S. , Sergeenkova Maria P. , Varlamova Elena V. , Antoshkina Evgeniya P. 
Published: September 2023

Abstract
This research focuses on analyzing how different primary catalysts affect the glycolysis of plastic made from bisphenol A (PC) polycarbonate. It was found that the process of chemical decomposition of PC leads to the formation of products with high added value: bisphenol A (PC monomer, BPA), BPA, and ethylene carbonate or ethylene glycol co-ethers (BPA monohydroxyethyl ether, MHE-BPA; BPA bishydroxyethyl ether, BHE-BPA). A quantitative assessment of the yields of the reaction products was also carried out. The yield of products at 100% PC conversion was 33% for BPA, 50% for MHE-BPA, and 17% for BHE-BPA. The effectiveness of using various alkalis depending on the type of metal was also compared within the context of this investigation.
KinD-LCE: Curve Estimation and Retinex Fusion on Low-Light Image
Authors: Lei Xiaochun , Mai Weiliang , Xie Junlin , Liu He , Jiang Zetao , Gong Zhaoting , Lu Chang , Lu Linjun 
Published: September 2023

Abstract
Low-light images often suffer from noise and color distortion. Object detection, semantic segmentation, instance segmentation, and other tasks are challenging when working with low-light images because of image noise and chromatic aberration. We also found that the conventional Retinex theory loses information in adjusting the image for low-light tasks. In response to the aforementioned problem, this paper proposes an algorithm for low illumination enhancement. The proposed method, KinD-LCE, uses a light curve estimation module to enhance the illumination map in the Retinex decomposed image, improving the overall image brightness. An illumination map and reflection map fusion module were also proposed to restore the image details and reduce detail loss. Additionally, a total variation loss function was applied to eliminate noise. The proposed method was trained using the GladNet dataset and tested with the Low-Light dataset. The ExDark dataset was used for validation in downstream tasks. The benchmark experiments demonstrated that the proposed algorithm achieved PSNR (19.7216) and SSIM (0.8213) values, which are close to state-of-the-art results.
Silver Nanoparticles as Potential Antibacterial and Antibiofilm Agents: Insights from Experimental and Computational Approaches
Authors: Alhadrami Hani A. , Elswifi Gamal H. , Rateb Mostafa E. , Hamd Ahmed 
Published: September 2023

Abstract
Background: In the present study we prepared silver nanoparticles (AgNPs) by the chemical reduction method which is considered more preferable than other previously reported methods. The prepared AgNPs were further evaluated for their antibacterial properties and their mode of action was putatively elucidated by molecular dynamics (MD) simulations.
Results: The structure and uniformity of the prepared AgNPs was confirmed by different characterization techniques including XRD, SEM, FT-IR, UV-vis spectroscopy, zeta potential, and DLS. Testing these AgNPs for their antibacterial and antibiofilm activities revealed very good potential against E. coli and K. pneumoniae (MIC = 37.5 and 18.75 μg/mL, respectively), P. aurigenosa and C. albicans (Percent of biofilm inhibition = 43% and 50%, respectively). Further comprehensive MD simulations revealed that the desorbed Ag atoms can bind with and probably inhibit the bacterial GSH reductase resulting in fatal increased oxidative stress inside the bacterial cell.
Conclusions: AgNPS produced by chemical methods are easy to prepare nanomaterial that exert considerable antibacterial effects via the inhibition of the bacterial GSH reductase. To the best of our knowledge, this is the first comprehensive MD simulation work that uncover the mechanism of the bacterial GSH reductase inhibition by Ag atoms. Accordingly, we believe that the results presented herein will pave the way for additional research into metal-protein interactions, which will aid in the development of new metal-based treatments.
ICD-11 Complex Posttraumatic Stress Disorder and Subclasses of Borderline Personality Disorder in a Korean General Population Sample with Childhood Abuse Experiences: A Latent Class Analysis
Authors: Lee Jisu , Choi Hyunjung 
Published: September 2023

Abstract
Background Complex posttraumatic stress disorder (CPTSD) and borderline personality disorder (BPD), which are distinctive diagnoses, share the common risk factor of childhood abuse experiences. However, additional evidence is needed to determine which factors contribute to the manifestation of different symptoms.
Method Participants were 499 Korean adults sampled from an online panel of a general population sample who reported experiences of childhood abuse. A latent class analysis (LCA) was conducted to identify the patterns of CPTSD and BPD symptoms. We adopted a three-step LCA to compare types of childhood abuse, invalidating environments, attachment styles, and pathological personality traits among different classes.
Results The LCA revealed four classes: a CPTSD and BPD “comorbid” class, an “externalizing BPD” class, an “avoidant BPD” class, and a low symptom class. The “comorbid” class showed the highest scores in all symptoms and risk factors. The “externalizing BPD” class was distinguished from the “avoidant BPD” class by the externalizing versus internalizing associated pathological personality traits. The “avoidant BPD” class experienced high emotional neglect in addition to other types of abuse and it also showed an additional avoidant attachment style.
Conclusion Childhood abuse may heighten the risk for high comorbidity of CPTSD and BPD as well as externalizing-internalizing subgroups of BPD. Beyond the identification of CPTSD and BPD, assessing attachment styles and pathological personality traits based on dimensional approaches would benefit the tailoring of effective treatment.