bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANALISIS PERLAKUAN AKUNTANSI ASET TETAP BERDASARKAN PERNYATAAN STANDAR AKUNTANSI KEUANGAN (PSAK) NOMOR 16 TENTANG ASET TETAP PADA PT. PERKEBUNAN NUSANTARA I (PERSERO) LANGSA
Authors: Inge Tasya Lonita  , Junita Afrah , Azhar Iqlima 
Published: February 2023

The research was conducted with the aim of knowing the accounting treatment of assets in accordance with PSAK No. 16, with the suitability of accounting for fixed assets at PT. Perkebunan Nusantara I (PERSERO) Langsa. The type of research used is qualitative. Sources of data used are primary and secondary data, and data collection methods obtained by using library research methods, interview methods, and documentation methods. The data analysis method used is a comparative descriptive analysis method. The results of this study indicate that in carrying out the accounting treatment of fixed assets PT. Perkebunan Nusantara I (PERSERO) Langsa it is not in accordance with from PSAK No. 16. The discrepancy regarding: the grouping of immature plantations (TBM) into fixed assets resulted in the value of fixed assets is not fair at PT. Perkebunan Nusantara I (PERSERO) Langsa. In addition, that causes the financial statements to be less reliable and relevant. The limitation of this study is that the authors only take to indicators of fixed asset accounting treatment, namely recognition and depreciation only. Given these limitations, the researches provides suggestions to further researches to expand research by adding indicators of fixed asset accounting measures such as measurement and disclosure.
Transitioning from epicutaneous to oral peanut immunotherapy
Authors: Wong Lauren , Kost Laurie , Anderson Brent , Long Andrew , Sindher Sayantani B. , Chinthrajah R. Sharon , Collins William J. 
Published: February 2023

IntroductionEpicutaneous immunotherapy (EPIT) has been tested in clinical trials for children with peanut allergy (PA) for its safety and efficacy in inducing desensitization. Aside from peanut avoidance and symptom management, oral immunotherapy (OIT) is another option for PA patients. However, OIT can be associated with adverse events and pose safety concerns to children and their caregivers.MethodsThis study assessed 27 children who successfully completed a peanut EPIT trial. 18 of them transitioned to peanut OIT with starting doses ranging from 10–600 mg of peanut protein. Our aim was to learn more about the EPIT to OIT experience through descriptive survey responses and to gather information that may support the sequential use of the two immunotherapies for safe and positive outcomes that may not be achieved by either alone.ResultsOverall, children and their caregivers had less anxiety about starting OIT after having had peanut exposure through EPIT. Most children who transitioned from EPIT to OIT had no or minor symptoms initially, with symptoms lessening later in OIT. Most were also able to maintain or increase their peanut dose over time, achieving maintenance doses of 60–2,000 mg.DiscussionIn comparison with current literature on OIT for PA in children, the reported symptoms appeared less severe and less prevalent in the EPIT to OIT group. However, there were 3 participants who withdrew from OIT due to the development of intolerable symptoms. This study provides initial data in support of EPIT to OIT, and larger randomized controlled trials assessing effectiveness of the two therapies together are warranted.
Isolation and screening of phosphorus solubilizing bacteria from saline alkali soil and their potential for Pb pollution remediation
Authors: Zhang Chaonan , Chen Haoming , Dai Yao , Chen Yan , Tian Yuxin , Huo Zongli 
Published: February 2023

The high pH and salinity of saline alkali soil not only seriously restrict the growth of crops, but also aggravate the pollution of heavy metals. The fixation of heavy metals and the regulation of pH by phosphorus solubilizing microorganisms may become a new way to repair heavy mental and improve saline alkali soil. In this study, a saline-alkali resistant bacteria (CZ-B1, CGMCC No: 1.19458) was screened from saline-alkali soil, and its tolerance to salt/alkali/lead stress was investigated by shaking flask experiment. The strain was identified as Bacillus amyloliquefaciens by morphology and 16S rRNA gene sequence analysis. The optimum growth temperature of CZ-B1 is about 35°C–40℃. The maximum salt stress and pH that it can tolerance are 100 g/L and 9 respectively, and its tolerance to Pb2+ can reach 2000 mg/L. The phosphorus release amount of CZ-B1 to Ca3(PO4)2 within 72 h is 91.00–102.73 mg/L. The phosphate solubilizing index in PVK agar medium and NBRIP agar medium are more than 2, which can be defined as phosphate solubilizing bacteria. Moreover, the dissolution of CZ-B1 to phosphorus is mainly attributed to tartaric acid, citric acid and succinic acid in inorganic medium. In addition, the removal rate of Pb2+ by CZ-B1 can reach 90.38% for 500 mg/L. This study found that CZ-B1 can immobilize Pb through three biological mechanisms (organic acid, extracellular polymers and mineralization reaction). The release of succinic acid (10.97 g/L) and citric acid (5.26 g/L) may be the main mechanism to promote the mineralization reaction of CZ-B1 (phosphate and oxalate) and resistance to Pb stress. In addition, the high enrichment of Pb2+ by EPS can increase the rate of extracellular electron transfer and accelerate the mineralization of CZ-B1. The screening and domestication of saline-tolerant phosphorus-solubilizing bacteria not only help to remediate Pb contamination in saline soils, but also can provide P element for plant growth in saline soil.
Gut microbiome and mycobiome in inflammatory bowel disease patients with Clostridioides difficile infection
Authors: Yu Si , Ge Xiaomeng , Xu Hui , Tan Bei , Tian Bowen , Shi Yujie , Dai Yimin , Li Yue , Hu Songnian , Qian Jiaming 
Published: February 2023

BackgroundClostridium difficile infection (CDI) is common in patients with inflammatory bowel disease (IBD) and has been reported as a risk factor for poor outcome. However, gut microbiome and mycobiome of IBD patients with CDI have been barely investigated. This study aimed to assess the gut microbiome and mycobiome in IBD patients with CDI.MethodsWe collected fecal samples from patients with active IBD and concomitant CDI (IBD-CDI group, n=25), patients with active IBD and no CDI (IBD-only group, n=51), and healthy subjects (HC, n=40). Patients’ characteristics including demographic data, disease severity, and medication history were collected. Metagenomic sequencing, taxonomic and functional analysis were carried out in the samples.ResultsWe found that the bacterial alpha diversity of the IBD-CDI group was decreased. The bacterial and fungal beta diversity variations between IBD patients and HC were significant, regardless of CDI status. But the IBD-CDI group did not significantly cluster separately from the IBD-only group. Several bacterial taxa, including Enterococcus faecium, Ruminococcus gnavus, and Clostridium innocuum were overrepresented in the IBD-CDI group. Furthermore, IBD patients with CDI were distinguished by several fungal taxa, including overrepresentation of Saccharomyces cerevisiae. We also identified functional differences in IBD patients with CDI include enrichment of peptidoglycan biosynthesis. The network analysis indicated specific interactions between microbial markers in IBD-CDI patients.ConclusionIBD patients with CDI had pronounced microbial dysbiosis. Gut micro-ecological changes in IBD patients with CDI might provide insight into the pathological process and potential strategies for diagnosis and treatment in this subset of patients.
Sex differences in heart failure patients assessed by combined echocardiographic and cardiopulmonary exercise testing
Authors: Rozenbaum Zach , Granot Yoav , Sadeh Ben , Havakuk Ofer , Arnold Joshua H. , Shimiaie Jason , Ghermezi Michael , Barak Orly , Ben Gal Yanai , Shacham Yacov , Keren Gad , Topilsky Yan , Laufer-Perl Michal 
Published: February 2023

BackgroundWe aimed to test the differences in peak VO2 between males and females in patients diagnosed with heart failure (HF), using combined stress echocardiography (SE) and cardiopulmonary exercise testing (CPET).MethodsPatients who underwent CPET and SE for evaluation of dyspnea or exertional intolerance at our institution, between January 2013 and December 2017, were included and retrospectively assessed. Patients were divided into three groups: HF with preserved ejection fraction (HFpEF), HF with mildly reduced or reduced ejection fraction (HFmrEF/HFrEF), and patients without HF (control). These groups were further stratified by sex.ResultsOne hundred seventy-eight patients underwent CPET-SE testing, of which 40% were females. Females diagnosed with HFpEF showed attenuated increases in end diastolic volume index (P = 0.040 for sex × time interaction), significantly elevated E/e' (P &lt; 0.001), significantly decreased left ventricle (LV) end diastolic volume:E/e ratio (P = 0.040 for sex × time interaction), and lesser increases in A-VO2 difference (P = 0.003 for sex × time interaction), comparing to males with HFpEF. Females diagnosed with HFmrEF/HFrEF showed diminished increases in end diastolic volume index (P = 0.050 for sex × time interaction), mostly after anaerobic threshold was met, comparing to males with HFmrEF/HFrEF. This resulted in reduced increases in peak stroke volume index (P = 0.010 for sex × time interaction) and cardiac output (P = 0.050 for sex × time interaction).ConclusionsCombined CPET-SE testing allows for individualized non-invasive evaluation of exercise physiology stratified by sex. Female patients with HF have lower exercise capacity compared to men with HF. For females diagnosed with HFpEF, this was due to poorer LV compliance and attenuated peripheral oxygen extraction, while for females diagnosed with HFmrEF/HFrEF, this was due to attenuated increase in peak stroke volume and cardiac output. As past studies have shown differences in clinical outcomes between females and males, this study provides an essential understanding of the differences in exercise physiology in HF patients, which may improve patient selection for targeted therapeutics.
Early Cretaceous volcanic-arc magmatism in the Dalat-Kratie Fold Belt of eastern Cambodia: implications for the lithotectonic evolution of the Indochina terrane
Authors: Shellnutt J. Gregory , Ma George S.-K. , Chan Jacky S.-L. , Wong Jean P.-M. , Wang Kuo-Lung 
Published: February 2023

Mesozoic granitic plutons are found throughout the Indochina terrane of eastern Cambodia and southern Vietnam. The granitic rocks range in age from Early Triassic (240 Ma) to Late Cretaceous (80 Ma) and record distinct tectonomagmatic periods associated with subduction of the Paleotethys and Paleo-Pacific oceans. Samples collected from the Snoul pluton, eastern Cambodia are composed of silicic and intermediate dioritic rocks, and basalt. The quartz diorites and diorites are magnesian, metaluminous, calcic to calc-alkalic, and similar to volcanic-arc granitoids whereas the basaltic rocks are compositionally similar to within-plate basalt. Zircon U-Pb geochronology and Lu-Hf isotopes and whole rock Sr-Nd isotopes show that the silicic rocks are Albian and isotopically juvenile (107.5 ± 0.3 Ma, 109.1 ± 0.4 Ma; εHf(t) = +7.0–+17.0; 87Sr/86Sri = 0.704313–0.707681; εNd(t) = +3.1–+4.9). Fractional crystallization modeling using a dioritic composition as the parental magma demonstrates that it is possible to generate the quartz diorite compositions under oxidizing (ΔFMQ +1) and hydrous (H2O = 2 wt%) conditions suggesting that they are consanguineous. The isotopically juvenile nature of the dioritic rocks and their compositional similarity (SiO2 ≥ 56 wt%, Al2O3 ≥ 15 wt%, Sr ≥ 400 ppm, Y ≤ 18 ppm, Yb ≤ 1.9 ppm) to adakitic rocks indicates that the parental magmas of the Snoul pluton were likely derived by partial melting of juvenile mafic basement rocks of the Indochina terrane. Moreover, Early Cretaceous plutonic rocks of Cambodia are isotopically distinct from plutonic rocks of similar age and tectonic setting from Vietnam suggesting that there could be a lithotectonic domain boundary within the Southern Indochina terrane. In contrast, the basaltic rocks likely record a temporally distinct period of magmatism associated with Late Cenozoic tensional plate stress.
Techno-economic analysis of the transition toward the energy self-sufficiency community based on virtual power plant
Authors: Wang Yafei , Gao Weijun , Li You , Qian Fanyue , Yao Wanxiang 
Published: February 2023

Distributed energy resources are important measures to increase energy self-sufficiency and overcome the global carbon reduction problem. However, individual planned renewable energy generation poses a significant threat to the power grid. Therefore, virtual power plant (VPP) is attracting considerable attention as a means of aggregating distributed energy in urban areas. This study proposed a VPP model consisting of updating high-efficiency appliances and photovoltaic and energy storage systems. A comprehensive analysis for assessing the technical, economic, and environmental benefits derived from the VPP was presented, indicating the feasibility of a smart community to achieve power self-sufficiency with the support of the VPP. A smart community in Japan was selected as the research object, with a peak power demand of 57,350 kW. The VPP’s load leveling performance, return on investment (ROI), and CO2 emission reduction were analyzed. In addition, external factors, such as electricity price changes and FiT policies, are considered to assess the impact on the economics of the VPP. The results show that the introduction of the VPP system in the community can effectively stabilize the grid load with a peak shaving rate reaching 42.55% and improve the energy self-sufficiency rate of the region reached 100%, besides providing superior economic and environmental benefits (16.26% CO2 emission reduction) on the demand side. Furthermore, the economic performance of VPP shows a good prospect with the fall in equipment prices and the future trend of carbon tax growth. This study provides important insights into the development of VPP in other countries, especially for low-energy self-sufficiency regions.
Characterization of early myocardial inflammation in ischemia-reperfusion injury
Authors: Wu Qihong , Xu Rong , Zhang Kun , Sun Ran , Yang Mengxi , Li Kuan , Liu Hanrui , Xue Yiyuan , Xu Huayan , Guo Yingkun 
Published: February 2023

BackgroundMyocardial injury may be caused by myocardial ischemia-reperfusion (IR), and salvaging such an injury is still a great challenge in clinical practice. This study comprehensively characterized the physiopathologic changes of myocardial injury after IR to explore the underlying mechanism in the early reperfusion phase with particular emphasis on early myocardial inflammation.Methods and ResultsThe experimental IR model was obtained by the left anterior descending artery’s transient ligation of C57BL/6 mice. T2W signals of all mice showed increased signal at different IR stages. It was positively correlated with inflammatory cytokines and cells. T2W imaging by 7.0 T MRI surprisingly detected signal enhancement, but histopathology and flow cytometry did not reveal any inflammatory cells infiltration within 3 h after IR. Cardiomyocyte swelling and increased vascular permeability were observed by WGA staining and ultrastructural analysis, respectively. The 3 h IR group showed that the cardiomyocytes were severely affected with disintegrating myofilaments and mitochondria. Both VEGF and phosphorylated Src protein were markedly expressed in the 3 h IR group in comparison with the sham group, and TUNEL staining displayed little positive cells. Cleaved caspase-3 apoptin also has similar expression levels with that of the sham group. Resident macrophages had notably become M1 phenotype. The T2W signal was still elevated, and we observed that collagen deposition occurred from 1 to 7 days.ConclusionsThe inflammation response during the first week after reperfusion injury gradually increase 3 h later, but the main manifestation before that was edema. This study indicated that the first 3 h may be crucial to the early rescue process for reperfusion-induced myocardial injury due to inflammatory cell infiltration absence and apoptosis.
Humoral and Cellular Immune Responses to SARS-CoV-2 mRNA Vaccination in Patients with Multiple Sclerosis: An Israeli Multi-Center Experience Following 3 Vaccine Doses
Authors: Milo Ron , Staun-Ram Elsebeth , Karussis Dimitrios , Karni Arnon , Hellmann Mark A. , Bar-Haim Erez , Miller Ariel ,   The Israeli Neuroimmunology Study Group on COVID-19 Vaccination in Multiple Sclerosis
Published: February 2023

BackgroundImmunomodulatory/immunosuppressive activity of multiple sclerosis (MS) disease modifying therapies (DMTs) might affect immune responses to SARS-CoV-2 exposure or vaccination in patients with MS (PwMS). We evaluated the effect of DMTs on humoral and cell-mediated immune responses to 2 and 3 vaccinations and the longevity of SARS-Cov-2 IgG levels in PwMS.Methods522 PwMS and 68 healthy controls vaccinated with BNT162b2-Pfizer mRNA vaccine against SARS-CoV-2, or recovering from COVID-19, were recruited in a nation-wide multi-center study. Blood was collected at 3 time-points: 2-16 weeks and ~6 months post 2nd vaccination and 1-16 weeks following 3rd vaccination. Serological responses were measured by quantifying IgG levels against the spike-receptor-binding-domain of SARS-CoV-2, and cellular responses (in a subgroup analysis) by quantifying IFNγ secretion in blood incubated with COVID-19 spike-antigen.Results75% PwMS were seropositive post 2nd or 3rd vaccination. IgG levels decreased by 82% within 6 months from vaccination (p&lt;0.0001), but were boosted 10.3 fold by the 3rd vaccination (p&lt;0.0001), and 1.8 fold compared to ≤3m post 2nd vaccination (p=0.025). Patients treated with most DMTs were seropositive post 2nd and 3rd vaccinations, however only 38% and 44% of ocrelizumab-treated patients and 54% and 46% of fingolimod-treated patients, respectively, were seropositive. Similarly, in COVID-19-recovered patients only 54% of ocrelizumab-treated, 75% of fingolimod-treated and 67% of cladribine-treated patients were seropositive. A time interval of ≥5 months between ocrelizumab infusion and vaccination was associated with higher IgG levels (p=0.039 post-2nd vaccination; p=0.036 post-3rd vaccination), and with higher proportions of seropositive patients. Most fingolimod- and ocrelizumab-treated patients responded similarly to 2nd and 3rd vaccination. IFNγ-T-cell responses were detected in 89% and 63% of PwMS post 2nd and 3rd vaccination, however in only 25% and 0% of fingolimod-treated patients, while in 100% and 86% of ocrelizumab-treated patients, respectively.ConclusionPwMS treated with most DMTs developed humoral and T-cell responses following 2 and 3 mRNA SARS-CoV-2 vaccinations. Fingolimod- or ocrelizumab-treated patients had diminished humoral responses, and fingolimod compromised the cellular responses, with no improvement after a 3rd booster. Vaccination following &gt;5 months since ocrelizumab infusion was associated with better sero-positivity. These findings may contribute to the development of treatment-stratified vaccination guidelines for PwMS.
Construction and validation of a cuproptosis-related prognostic model for glioblastoma
Authors: Zhang Bohong , Xie Lin , Liu Jiahao , Liu Anmin , He Mingliang 
Published: February 2023

BackgroundCuproptosis, a newly reported type of programmed cell death, takes part in the regulation of tumor progression, treatment response, and prognosis. But the specific effect of cuproptosis-related genes (CRGs) on glioblastoma (GBM) is still unclear.MethodsThe transcriptome data and corresponding clinical data of GBM samples were downloaded from the TCGA and GEO databases. R software and R packages were used to perform statistical analysis, consensus cluster analysis, survival analysis, Cox regression analysis, Lasso regression analysis, and tumor microenvironment analysis. The mRNA and protein expression levels of model-related genes were detected by RT-qPCR and Western blot assays, respectively.ResultsThe expression profile of CRGs in 209 GBM samples from two separate datasets was obtained. Two cuproptosis subtypes, CRGcluster A and CRGcluster B, were identified by consensus cluster analysis. There were apparent differences in prognosis, tumor microenvironment, and immune checkpoint expression levels between the two subtypes, and there were 79 prognostic differentially expressed genes (DEGs). According to the prognostic DEGs, two gene subtypes, geneCluster A and geneCluster B, were identified, and a prognostic risk score model was constructed and validated. This model consists of five prognostic DEGs, including PDIA4, DUSP6, PTPRN, PILRB, and CBLN1. Ultimately, to improve the applicability of the model, a nomogram was established. Patients with GBM in the low-risk cluster have a higher mutation burden and predict a longer OS than in the high-risk group. Moreover, the risk score was related to drug sensitivity and negatively correlated with the CSC index.ConclusionWe successfully constructed a cuproptosis-related prognostic model, which can independently predict the prognosis of GBM patients. These results further complement the understanding of cuproptosis and provide new theoretical support for developing a more effective treatment strategy.
Estrogen-related genes influence immune cell infiltration and immunotherapy response in Hepatocellular Carcinoma
Authors: Gao Biao , Wang Yafei , Li Chonghui , Lu Shichun 
Published: February 2023

BackgroundImmunotherapy has been the first-line treatment option in advanced Hepatocellular Carcinoma(HCC); but now, there are no established molecular markers that can predict immunotherapy response. Estrogen has a crucial role in the development of a variety of liver illnesses, including liver fibrosis, Nonalcoholic fatty liver disease (NAFLD), and HCC. Nonetheless, the significance of estrogen-related genes in HCC immunotherapy and the underlying molecular mechanisms are not yet fully understood.MethodIn this study, we constructed a novel estrogen-related gene prognostic signature (ERGPS) by analyzing bulk RNA sequencing data from 365 HCC patients. Based on the median risk score, we divided 365 HCC patients into low- and high-risk groups. Tumor mutation burden (TMB), Microsatellite instability (MSI), T cell receptor (TCR) richness, B cell receptor (BCR) richness, single-nucleotide variants (SNV) Neoantigens, Cancer Testicular Antigens (CTA) scores, and Tumour Immune Dysfunction and Exclusion (TIDE) scores were used to evaluate the magnitude of immunotherapy response. Multiple external datasets validate the validity and robustness of the prognostic signature. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to validate estrogen-related gene overexpression in HCC tissue samples.ResultsERGPS is an independent risk factor affecting the prognosis of HCC patients and is superior to other clinical variables in predicting patient survival and immunotherapy response. Multiple independent external datasets confirmed the superior predictive efficacy of the prognostic signature. The prognostic signature was positively correlated with TMB score, MSI score, TCR richness, BCR richness, SNV Neoantigens score, CTA score, expression levels of immune checkpoint-related genes, and TIDE score. Patients with HCC in the high-risk group identified by the prognostic signature were likely to be more responsive to immunotherapy and more suitable for immunotherapy. qRT-PCR confirmed that estrogen-related genes of the construct signature were highly expressed in HCC tumor tissues.ConclusionEstrogen-related genes are overexpressed in HCC tissues. Our novel prognostic signature can accurately predict not only the prognosis but also the immunotherapy response of HCC patients. In the future, prognostic signatures will be a useful tool for clinicians to screen patients with HCC who are suitable for immunotherapy.