bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bovine leukemia virus-derived long-noncoding RNA, AS1-S, binds to bovine hnRNPM and alters interaction properties between hnRNPM and host mRNAs
Authors: Andoh Kiyohiko , Nishimori Asami , Matsuura Yuichi 
Published: March 2023

AbstractViruses utilize several strategies to develop latent infection and evade host immune responses. Long non-coding RNA (lncRNA), a class of non-protein encoding RNA that regulates various cellular functions by interacting with RNA binding proteins, is a key factor for viral latency because of its lack of antigenicity. Bovine leukemia virus (BLV), which belongs to the family Retroviridae, encodes the BLV-derived lncRNA AS1-S, which is a major transcript expressed in latently infected cells. We herein identified bovine hnRNPM, an RNA-binding protein located in the nucleus, as the binding partner for AS1-S using an RNA-protein pull-down assay. The pull-down assay using recombinant hnRNPM mutants showed that RNA recognition motif 1 and 2, located in the N-terminal region of bovine hnRNPM, are responsible for binding AS1-S. Furthermore, an RNA immunoprecipitation assay showed that introduction of AS1-S increased the number of mRNA that co-immunoprecipitated with bovine hnRNPM in MDBK cells. These results suggested that AS1-S could alter the interaction between hnRNPM and host mRNAs, potentially interfering with cellular functions during the initial phase of mRNA maturation in the nucleus. Since most of the identified mRNAs that exhibited increased binding to hnRNPM were correlated with the KEGG term “Pathways in cancer”, AS1-S may affect proliferation and expansion of BLV-infected cells and contribute to tumor progression.ImportanceBLV infects bovine B cells and causes malignant lymphoma, resulting in severe economic losses in the livestock industry. Due to its low incidence rate and long latent period, the molecular mechanisms underlying the progression to lymphoma remain enigmatic. Several non-coding RNAs, such as miRNA and lncRNA, have recently been discovered in the BLV genome and the relationship between BLV pathogenesis and these non-coding RNAs is attracting attention. However, most of the molecular functions of these transcripts remain un-identified. To the best of our knowledge, this is the first report describing a molecular function for the BLV-derived lncRNA AS1-S. The findings reported herein reveal a novel mechanism underlying BLV pathogenesis that could provide important insights for not only BLV research but also comparative studies of retroviruses.
T1R3 subunit of the sweet taste receptor is activated by D2O in transmembrane domain-dependent manner
Authors: Abu Natalie Ben , Shoshan-Galeczki Yaron Ben , Malach Einav , Niv Masha Y. 
Published: March 2023

AbstractDeuterium oxide (D2O) is a water molecule in which both hydrogens are replaced by the heavier and rare isotope deuterium. We have previously shown that D2O has distinct sweet taste, which is mediated by the T1R2/T1R3 sweet taste receptor. Here we explore the effect of heavy water on T1R2 and T1R3 subunits. We show that D2O activates T1R3 transfected HEK293T cells similarly to T1R2/T1R3 transfected cells. The response to glucose dissolved in D2O is higher than to glucose dissolved in water. Mutations of Phenylalanine at position 7305.40in the transmembrane domain of T1R3 to Alanine, Leucine or Tyrosine, impair or diminish activation by D2O, suggesting a critical role for T1R3 TMD domain in relaying the heavy water signal.
Application of a Machine Learning Approach Towards the Targeted Identification of Phage Depolymerases
Authors: Magill Damian J. , Skvortsov Timofey A. 
Published: March 2023

AbstractBiofilm production plays a clinically significant role in the pathogenicity of many bacteria, limiting our ability to apply antimicrobial agents and contributing in particular to the pathogenesis of chronic infections. Bacteriophage depolymerases, leveraged by these viruses to circumvent biofilm mediated resistance, represent a potentially powerful weapon in the fight against antibiotic resistant bacteria. Such enzymes are able to degrade the extracellular matrix that is integral to the formation of all biofilms and as such would allow complementary therapies or disinfection procedures to be successfully applied. In this manuscript, we describe the development and application of a machine learning based approach towards the identification of phage depolymerases. We demonstrate that on the basis of a relatively limited number of experimentally proven enzymes and using an amino acid derived feature vector that the development of a powerful model with an accuracy on the order of 90% is possible, showing the value of such approaches in the discovery of novel therapeutic agents.
Methionine and Iron Supplementation Enhances Kanamycin Activity AgainstMycobacterium tuberculosis
Authors: Paritala Hanumantharao , Carroll Kate S. 
Published: March 2023

AbstractDespite understanding the functional roles of important proteins in sulfate assimilation and trans-sulfuration pathways, no study has been conducted to estimate the changes in the intracellular thiol levels when mycobacterium is treated with methionine. Methionine was known to be sulfur source for mycobacteria when it resides in the hostile granuloma environment and through transsufuration pathway methionine was shown to stimulate the sulfate assimilation. To study the dynamics of sulfate assimilation pathway we created gene knockout strains of important enzymes in the sulfate assimilation pathway of M. smegmatis. We stimulated these mutants with methionine, pro-filed the changes in intracellular thiols, applied external oxidative stress, and monitored the associated intracellular redox potentials (EMSH). These experiments revealed that when M. smegmatis cultures are supplemented with methionine intracellular levels surged. When those cells were challenged with hydrogen peroxide the M. smegmatis cells showed increased intracellular oxidative potentials and took prolonged time to revert back normal redox balance state when compared to non-methionine containing controls. Here in this report, we are describe how the dynamic changes of intracellular thiols are linked to augment the activity of kanamycin.
Exploring predictors of e-marketing continuance intention in the Zimbabwean pharmaceutical industry during the COVID-19 pandemic
Authors: Aigbogun Osaro , Matinari Mathews , Fawehinmi Olawole 
Published: March 2023

PurposeThe purpose of this study is to empirically explore the predictors of e-marketing use continuance intention in the pharmaceutical business to business (B2B) supply chain during the COVID-19 pandemic.Design/methodology/approachThis study adopted survey research strategy, and data were collected from managers dealing with marketing in 127 pharmaceutical firms in Harare Zimbabwe using a self-reported questionnaire. Partial least squares structural equation modeling (PLS-SEM) was employed to test the hypotheses.FindingsLeadership support and perceived usefulness are significant predictors of e-marketing continuance intentions. The effect of perceived susceptibility and perceived severity on e-marketing use continuance intention was not significant. Perceived usefulness is a positive moderator in the relationship among leadership support, perceived susceptibility and e-marketing use continuance intention. However, the moderating effect of perceived usefulness created a significant but negative relationship between perceived severity and e-marketing use continuance intention.Originality/valueThis study provides empirical evidence of the moderating role of perceived usefulness in the relationships between e-marketing continuance intention and its predictors.
Metaverse academic library: would it be patronized?
Authors: Adetayo Adebowale Jeremy , Adekunmisi Sowemimo Ronke , Abata-Ebire Blessing Damilola , Adekunmisi Adedokun Adedayo 
Published: March 2023

Purpose
This study aims to examine if students would patronize metaverse academic library (MAL) if it becomes available in Nigeria.

Design/methodology/approach
This study used a descriptive survey research design. The population was made up of 1,037 undergraduate in Adeleke University. Descriptive statistic was used to analyse data.

Findings
Findings indicated most students have never used virtual reality (VR) equipment, they are eager to use MAL for virtual academic research, library user education, accessing circulation services, reading serials and contacting reference librarians. The study concluded that MAL would be patronised by students if it is made available and therefore recommends that greater effort be made to make VR gear accessible and cheap for developing nations such as Nigeria.

Originality/value
The study is novel as it contributes to scarce research on MAL.
Bank opacity and stability in an emerging market
Authors: Dang Van Dan , Huynh Japan 
Published: March 2023

PurposeThe paper analyzes the impact of bank opacity on financial stability in an emerging economy.Design/methodology/approachBased on a unique dataset of 31 Vietnamese commercial banks from 2007 to 2019, the paper captures earnings opacity via discretionary loan loss provisions and reflects individual bank stability through the accounting-based Z-score index and its disaggregate components. The least squares dummy variable corrected (LSDVC) approach is employed for empirical analysis.FindingsIn contradiction to most studies on developed economies, earnings management improves bank financial stability in Vietnam. Earnings management is more important for the financial stability of smaller banks. Further, the effect of financial information disclosure on bank stability is strengthened by unfavorable macroeconomic conditions, particularly economic downturns, the global financial crisis and uncertain times in banking.Originality/valueThis is the first study to shed light on how bank opacity influences bank financial stability in an emerging market. The evidence with the conditioning roles of bank size and macroeconomic factors, such as uncertainty in banking, is entirely novel in the related literature. Additionally, the paper contributes to a growing body of banking literature by using the LSDVC estimator to examine the association between bank opacity and bank stability.
Error identification and compensation of 1T2R parallel power head based on trajectory optimization and principal component analysis
Authors: Ni Yanbing , Cui Yizhang , Jia Shilei , Lu Chenghao , Lu Wenliang 
Published: March 2023

Purpose
The purpose of this paper is to propose a method for selecting the position and attitude trajectory of error measurement to improve the kinematic calibration efficiency of a one translational and two rotational (1T2R) parallel power head and to improve the error compensation effect by improving the properties of the error identification matrix.

Design/methodology/approach
First, a general mapping model between the endpoint synthesis error is established and each geometric error source. Second, a model for optimizing the position and attitude trajectory of error measurement based on sensitivity analysis results is proposed, providing a basis for optimizing the error measurement trajectory of the mechanism in the working space. Finally, distance error measurement information and principal component analysis (PCA) ideas are used to construct an error identification matrix. The robustness and compensation effect of the identification algorithm were verified by simulation and through experiments.

Findings
Through sensitivity analysis, it is found that the distribution of the sensitivity coefficient of each error source in the plane of the workspace can approximately represent its distribution in the workspace, and when the end of the mechanism moves in a circle with a large nutation angle, the comprehensive influence coefficient of each sensitivity is the largest. Residual analysis shows that the robustness of the identification algorithm with the idea of PCA is improved. Through experiments, it is found that the compensation effect is improved.

Originality/value
A model for optimizing the position and attitude trajectory of error measurement is proposed, which can effectively improve the error measurement efficiency of the 1T2R parallel mechanism. In addition, the PCA idea is introduced. A least-squares PCA error identification algorithm that improves the robustness of the identification algorithm by improving the property of the identification matrix is proposed, and the compensation effect is improved. This method has been verified by experiments on 1T2R parallel mechanism and can be extended to other similar parallel mechanisms.
Metaverse office: exploring future teleworking model
Authors: Chen Zhisheng 
Published: March 2023

PurposeThis study aims to explore the application scenarios of metaverse offices in organizations, including immersive work experiences, weak social networks and virtual meetings. Based on the exploration of this application scenario, this study discusses four levels of organizational change that will be brought about by the future metaverse office, such as the inter-enterprise ecological collaboration model, distributed autonomous organization, flexible leadership and individual belongingness. The metaverse office has some special advantages over traditional office models, but the metaverse office still raises some issues, such as privacy, security, addiction, equity and usability. These issues brake the widespread adoption of metaverse technologies. The study recommends that researchers take these issues into account in future metaverse research and development.Design/methodology/approachThis study surveys the relevant literature by means of a literature review in order to analyze how metaverse technology can be applied to teleworking. Two databases including Web of Science (https://www.webofscience.com/) and Google Scholar (http://scholar.google.com) were selected for this study. Keywords such as “teleworking and metaverse” were used, and 18 publications were found to be relevant to the study. After excluding duplicates, less relevant and older literature, only 14 articles could be used as references for this study. Based on the exploration of this application scenario, this study discusses four levels of organizational change and issues that will be brought about by the metaverse office of the future.FindingsIt presents the application scenarios of the metaverse office in organizations, including immersive work experience, weak social networking and virtual meetings.Social implicationsThe pandemic triggers a desire for contactless working. The three main applications of the metaverse office also have a practical value which has been proven in some high-tech companies. It is foreseeable that an efficient, electronic and personalized office model will be enthusiastically adopted by society. Metaverse office will gradually step into people's view in the future.Originality/valueThe concept of the metaverse office has rarely been touched upon by theoretical research, although the technology is gradually becoming known. Even its application to teleworking has only recently been reached by some organizations.
A terrible beauty is burned: gifts, sacrifice and community in Northern Ireland
Authors: Downey Hilary , Sherry, Jr John F. 
Published: March 2023

PurposeSacrifice, integral to gift giving, remains unexplored and undertheorized in marketing. This paper aims to address this shortfall by analyzing the dynamics of sacrifice and theorizing how it serves as an engine of the gift chimney.Design/methodology/approachThe ethnographic investigation of public ceremonial gift giving in sectarian Northern Ireland describes and interprets the complex nature of the gift.FindingsThe authors show that sacrifice is a plausible mechanism of the gift chimney and that the co-occurrence of monadic, dyadic and systemic giving in the same ritual acts as an accelerant.Social implicationsThe authors analyze how public ceremonial gift giving induces sectarian communities to risk convocation, enabling them to exorcize trauma sustained at one another’s hands and to build a platform for future cross-community cohesion in a context of ineffective institutional efforts.Originality/valueSacrifice propels circulation of the gift, creating a social bond between antagonists whose ethos of mutuality depends upon ritualized reciprocal recognition of entangled loss.
Mechanisms of iron homeostasis in Pseudomonas aeruginosa and emerging therapeutics directed to disrupt this vital process
Authors: Sánchez‐Jiménez Ana , Marcos‐Torres Francisco J. , Llamas María A. 
Published: March 2023

AbstractPseudomonas aeruginosa is an opportunistic pathogen able to infect any human tissue. One of the reasons for its high adaptability and colonization of host tissues is its capacity of maintaining iron homeostasis through a wide array of iron acquisition and removal mechanisms. Due to their ability to cause life‐threatening acute and chronic infections, especially among cystic fibrosis and immunocompromised patients, and their propensity to acquire resistance to many antibiotics, the World Health Organization (WHO) has encouraged the scientific community to find new strategies to eradicate this pathogen. Several recent strategies to battle P. aeruginosa focus on targeting iron homeostasis mechanisms, turning its greatest advantage into an exploitable weak point. In this review, we discuss the different mechanisms used by P. aeruginosa to maintain iron homeostasis and the strategies being developed to fight this pathogen by blocking these mechanisms. Among others, the use of iron chelators and mimics, as well as disruption of siderophore production and uptake, have shown promising results in reducing viability and/or virulence of this pathogen. The so‐called ‘Trojan‐horse’ strategy taking advantage of the siderophore uptake systems is emerging as an efficient method to improve delivery of antibiotics into the bacterial cells. Moreover, siderophore transporters are considered promising targets for the developing of P. aeruginosa vaccines.
Prospective, open‐label, and observational study of cetuximab for metastatic colorectal carcinoma: The OPTIM1SE study
Authors: Yang Tsai‐Sheng , Chen Hong‐Hwa , Bo‐Wen Lin , Kim Tae Won , Kim Jong Gwang , Ahn Joong Bae , Lee Myung‐Ah , Lin Johnson , Ho Gwo Fuang , Anh Le Tuan , Temraz Sally , Burge Matthew , Chua Clarinda , Huang Jason , Park Young Suk 
Published: March 2023

AbstractAimThe OPTIM1SE study observed long‐term real‐world outcomes of cetuximab‐based infusional 5‐fluorouracil (5‐FU) regimens for first‐line treatment of metastatic colorectal cancer (mCRC) across Asia‐Pacific and Middle East regions, aiming to characterize their use, effectiveness, and safety in routine practice.MethodsOPTIM1SE was a prospective, open‐label, observational study. Patients with untreated KRAS wild‐type mCRC and distant metastases were treated per locally approved labels and monitored for 3 years via electronic medical records. The primary endpoint was the overall response rate (ORR). Secondary endpoints included safety, progression‐free survival (PFS), and overall survival (OS).ResultsFrom November 19, 2013, to June 30, 2016, 520 patients were enrolled in 51 sites. Patients were mostly male (61.2%), with a mean age of 58.5 (±12.0) years; 420 patients received leucovorin, 5‐FU, and irinotecan–based regimens and 94 received leucovorin, 5‐FU, and oxaliplatin. The most common primary tumor site was the rectum (38.8%), with liver metastases (65.0%). ORR was 45.4% (95% CI, 41.1%–49.7%), including 26 patients (5.0%) with a complete response. Median PFS was 9.9 months (95% CI, 8.2–11.0); median OS (mOS) was 30.8 months (95% CI, 27.9–33.6). Higher mOS was associated with tumors of left compared with right‐sided origin (hazard ratio, 0.69 [95% CI, 0.49–0.99]); higher ORR was also associated with liver metastases compared with all other metastases (55.4% vs. 40.2%). Adverse events were consistent with the known safety profile of cetuximab.ConclusionCetuximab‐based 5‐FU regimens were effective first‐line treatments for mCRC in routine practice, particularly in patients with left‐sided disease and liver metastases only.
Does habitat type in no‐tillage agroecosystems influence ground‐dwelling macroarthropod community structure? A case study in KwaZulu‐Natal, South Africa
Authors: Mwabvu Tarombera , Nxele Thembeka , Yekwayo Inam 
Published: March 2023

AbstractLoss of biodiversity in agroecosystems has negative implications for crop production and the functioning of the ecosystem. As such, agroecologists advocate minimum tillage practices in order to conserve biological diversity. To establish the influence of agricultural practices on biodiversity, we compared the species richness, abundance and composition of beetles, ants, spiders and small groups of ground dwelling arthropods in a veld and three agroecosystems (maize, sugarcane and multispecies pasture) under minimum tillage. Sampling was by pitfall trapping during the summer rainfall season in December 2019. Although ants were the most abundant (47%), followed by beetles (31%); beetles were the most speciose making up 40% of the morphospecies followed by spiders (31%). The veld had significantly greater relative proportion of species richness of spiders (54%) than the three agroecosystems, while significantly low relative proportion of species richness of ants was in the pasture (6%) compared to the veld (63%). Species richness and abundance of beetles were similar across habitat types. The four habitats supported different assemblages of ants, beetles and spiders. The differences in species composition among the habitats support the notion that agricultural practices with minimum soil disturbance enhance conservation of biological diversity, particularly, specialist species.
Effect of pneumatic leg compression on post‐induction hypotension in elderly patients undergoing robot‐assisted laparoscopic prostatectomy: a double‐blind randomised controlled trial
Authors: Park J.‐Y. , Yu J. , Kim C.‐S. , Baek J.‐W. , Jo Y. , Kim Y.‐K. 
Published: March 2023

SummaryPost‐induction hypotension is common and associated with postoperative complications. We hypothesised that pneumatic leg compression reduces post‐induction hypotension in elderly patients undergoing robot‐assisted laparoscopic prostatectomy. In this double‐blind randomised study, patients were allocated randomly to the pneumatic leg compression group (n = 50) or control (n = 50). In the intervention group, pneumatic leg compression was initiated before induction of anaesthesia. In the control group, pneumatic leg compression was initiated 20 min after anaesthesia induction. The primary outcome was the incidence of post‐induction hypotension in these groups. Post‐induction hypotension was defined as systolic blood pressure < 90 mmHg during the first 20 min after induction. Haemodynamic variables and area under the curve of post‐induction systolic blood pressure over time were assessed. Complications associated with pneumatic leg compression were recorded, including: peripheral neuropathy; compartment syndrome; extensive bullae beneath the leg sleeves; and pulmonary thromboembolism. The incidence of post‐induction hypotension decreased in the pneumatic leg compression group compared with that in the control group; 5 (10%) vs. 29 (58%), respectively, p < 0.001. In the pneumatic leg compression group, the lowest systolic, diastolic and mean blood pressures 20 min after induction of anaesthesia were significantly greater than the control group. Pneumatic leg compression resulted in an increased area under the curve of systolic blood pressure in the first 20 min after induction, p = 0.001. There were no pneumatic leg compression‐related complications. Pneumatic leg compression reduced post‐induction hypotension in elderly patients undergoing robot‐assisted laparoscopic prostatectomy, suggesting that it is an effective and safe intervention to prevent post‐induction hypotension among elderly patients undergoing general anaesthesia.