bluuebunny/crossref_metadata_2025_split huggingface dataset:
The other colon cancer: a population‐based cohort study of appendix tumour trends and prognosis
Authors: Halfter Kathrin , Schubert‐Fritschle Gabriele , Klauschen Frederick , Werner Jens , Mayerle Julia , Weichert Wilko , Friess Helmut , Schmid Roland , Kremer Marcus , Ruppert Reinhard , Hoelzl Jürgen , Krenz Detlef , Nerlich Andreas , Agha Ayman , Fuchs Martin , Becker Ingrid , Nowak Kai , Engel Jutta , Schlesinger‐Raab Anne 
Published: February 2023

AbstractAimAppendiceal neoplasms are rare subtypes of colorectal tumours that mainly affect younger patients some 20 years earlier than other colon tumours. The aim of this study was to gain more insight into the histological subtypes of this rare disease and include cases previously excluded, such as mucinous neoplasia.MethodThe cohort study included 1097 patients from the Munich Cancer Registry (MCR) diagnosed between 1998 and 2020. Joinpoint analysis was used to determine trend in incidence. Baseline demographic comparisons and survival analyses using competing risk and univariate/multivariate methods were conducted according to tumour histology: adenocarcinoma (ADENO), neuroendocrine neoplasia (NEN), mixed adeno‐neuroendocrine carcinoma (MANEC), and low‐ (LAMN) and high‐grade mucinous neoplasia (HAMN).ResultsUp to 2016 the number of cases increased significantly [annual per cent change (APC) = 6.86, p < 0.001] followed by a decline in the following years (APC = −14.82, p = 0.014; average APC = 2.5, p = 0.046). Comparison of all patients showed that NEN (48.4%) and mucinous neoplasms (11.6%) had a considerably better prognosis than ADENO (36.0%) and MANEC (3.0%, p < 0.0001). A multivariate analysis within the NEN and ADENO subgroups revealed that further histological classification was not prognostically relevant, while older age and regional tumour spread at diagnosis were associated with a poor prognosis. ADENO histology with high tumour grade and appendectomy only was also associated with poorer survival.ConclusionAppendiceal neoplasms are histologically heterogeneous; however, this diversity becomes less relevant compared with the marked difference from cancers of the remaining colon. The previously observed increase in cases appears to be abating; fewer cases of appendicitis and/or appendectomies or changes in histopathological assessment may be behind this trend.
Successful treatment of laryngeal dysplasia with ventriculocordectomy and prosthetic laryngoplasty in a horse
Authors: Morelli Chiara , Rubio‐Martínez Luis M. 
Published: February 2023

SummaryProsthetic laryngoplasty is a recognised treatment option for recurrent laryngeal neuropathy (RLN) in horses; however, there is no report of the use of laryngoplasty alone or in combination, as a successful treatment of equine laryngeal dysplasia. A 6‐year‐old Warmblood gelding was referred to the hospital for investigation of respiratory noise and exercise intolerance. Grade 4 recurrent laryngeal neuropathy and bilateral vocal fold collapse were diagnosed with overground endoscopy. Ultrasonography revealed laryngeal dysplasia. The horse underwent standing laser bilateral ventriculectomy, left vocal cordectomy, right vocal cordotomy and left‐sided laryngoplasty. Abnormal structural anatomy of the left aspect of the larynx consistent with laryngeal dysplasia was identified during surgery. Post‐operatively, the horse showed substantial clinical improvement with decreased respiratory noise and improved exercise capacity. Follow‐up overground endoscopy at 6 months post‐operative revealed Dixon grade 4 abduction of the left arytenoid cartilage. Follow‐up information via telephone conversation with the owner confirmed that the horse continued in active exercise with clinical improvement. This case report highlights the value of combined clinical, endoscopic and ultrasonographic examinations of the larynx in a horse with laryngeal dysplasia and concurrent grade 4 RLN and the successful surgical treatment achieved with combined bilateral ventriculectomy, left vocal cordectomy, right vocal cordotomy and prosthetic laryngoplasty.
The McMaster Family Assessment Device (FAD) dimensions involved in the prediction of adolescent depressive symptoms and their mediating role in regard to socioeconomic status
Authors: Caño González Antonio , Rodríguez‐Naranjo Carmen 
Published: February 2023

AbstractFamily functioning plays an important role in explaining the high prevalence of depressive symptoms in adolescents and it is necessary to identify the family functioning characteristics responsible for this relationship. In turn, while socioeconomic status (SES) is associated with adolescent depressive symptoms, the mechanisms that explain this relationship are largely unknown. In this study, we used the McMaster Family Assessment Device (FAD) to obtain a picture of the family functioning dimensions that genuinely contribute to explaining the relationship between family functioning and adolescent depressive symptoms and analyzed the mediating effect of family functioning on the impact of SES on depressive symptoms. Regression‐based conditional process analysis was used with a sample of 636 adolescents aged 12–17 years. Pratt's measures in regression analyses showed that 95% of the variance in depressive symptoms was accounted for by three of the six FAD dimensions: the ability to experience and express emotions appropriately—Affective Responsiveness—the ability to maintain adequate involvement among family members—Affective Involvement—and the ability to set and abide by rules and standards of behavior—Behavioral Control. Results also showed that the impact of SES on depressive symptoms was mediated by the existence of clear expectations about standards of behavior and behavioral patterns for handling family tasks—Behavioral Control and Roles—and, for the boys, by experiencing and expressing emotions appropriately. The results emphasize the importance of affect and clear‐cut family rules to prevent adolescent depressive symptoms and suggest that the existence of family rules and roles buffer the impact of SES on adolescent wellbeing.
Novel double staining of the stratum corneum with fluorescent ε‐poly‐l‐lysine and anionic dextran
Authors: Yoshida Moemi , Numajiri Sana , Notani Nao , Nomoto Koji , Urabe Hiroya , Sato Jun‐ichi , Yamashita Yuji , Hirao Tetsuji 
Published: February 2023

AbstractObjectiveε‐Poly‐l‐lysine (PLL) is a cationic polymer consisting of 25–35 l‐lysine residues. Our previous study revealed that fluorescently labelled PLL can stain the stratum corneum (SC) via ionic interactions between PLL and SC constituents. In this study, to further clarify the mechanisms underlying the interaction between PLL and the SC, the staining properties of fluorescent PLL were compared with that of fluorescently labelled anionic dextran (aDex), which has approximately the same molecular weight as PLL.MethodsSC samples were collected by non‐invasive tape stripping and stained with fluorescent PLL and/or fluorescent aDex. Fluorescence images were acquired using a fluorescence microscope and then analysed.ResultsThe SC could be stained with either fluorescent PLL or aDex, both of which were inhibited by the addition of high concentrations of salt solutions. In particular, aDex staining was inhibited at a lower salt concentration than PLL staining. Moreover, PLL staining was inhibited under acidic conditions, while aDex staining was inhibited under neutral to alkaline conditions. Double staining of SC with both fluorescent polymers produced heterogeneous staining patterns: corneocytes stained with both polymers, corneocytes stained with PLL or aDex in a mutually exclusive manner, and unstained corneocytes. Staining of SC samples from the face was more extensive than staining of SC samples from the inside of the upper arm with both polymers. In addition, pretreatment of the SC with ethanol resulted in enhanced staining with both polymers. These results suggest that double staining of SC with both polymers can provide information on the damaged SC.ConclusionStaining of SC with fluorescent PLL depends on its properties of a cationic and hydrophobic polymer with appropriate molecular size, which can distinguish the damaged SC. Double staining of SC with fluorescent PLL and aDex is a novel approach to obtain information for the analysis of skin conditions.
Attrition rate in infant fNIRS research: A meta‐analysis
Authors: Baek Sori , Marques Sabrina , Casey Kennedy , Testerman Meghan , McGill Felicia , Emberson Lauren 
Published: February 2023

AbstractUnderstanding the trends and predictors of attrition rate, or the proportion of collected data that is excluded from the final analyses, is important for accurate research planning, assessing data integrity, and ensuring generalizability. In this pre‐registered meta‐analysis, we reviewed 182 publications in infant (0–24 months) functional near‐infrared spectroscopy (fNIRS) research published from 1998 to April 9, 2020, and investigated the trends and predictors of attrition. The average attrition rate was 34.23% among 272 experiments across all 182 publications. Among a subset of 136 experiments that reported the specific reasons for subject exclusion, 21.50% of the attrition was infant‐driven, while 14.21% was signal‐driven. Subject characteristics (e.g., age) and study design (e.g., fNIRS cap configuration, block/trial design, and stimulus type) predicted the total and subject‐driven attrition rates, suggesting that modifying the recruitment pool or the study design can meaningfully reduce the attrition rate in infant fNIRS research. Based on the findings, we established guidelines for reporting the attrition rate for scientific transparency and made recommendations to minimize the attrition rates. This research can facilitate developmental cognitive neuroscientists in their quest toward increasingly rigorous and representative research.
Risk factors associated with keloid infections: A five‐year retrospective study
Authors: Liang Zhengyun , Zhang Mingzi , Hao Yan , Shan Mengjie , Liu Hao , Xia Yijun , Chen Qiao , Chang Guojing , Wang Youbin 
Published: February 2023

AbstractKeloid infections reduce patient‐reported quality of life greatly. Characteristics and risk factors of keloid infections have not been thoroughly studied. So, a retrospective cohort study was conducted focusing on the potential risk factors, microbiologic cultures and histological findings. Keloid patients consulting for surgical interventions were included in this study. Data were collected from their electronic medical records. 564 patients were recruited with the keloid infection rate being 22.4%. For adult patients, age above 40 years (OR, 2.84; P = .000), disease duration of 12 years or more (OR, 3.03; P = .000), the number of keloids over 3 (OR, 1.59; P = .050) and the presence of family history (OR, 1.91; P = .027) were significantly associated with keloid infections. Suppurative keloids were located mostly in thorax (61.79%). For the under‐age subgroup(n = 25), family history was frequently seen in patients with infections. Microbiologic cultures revealed a mixed spectrum of bacteria including Staphylococcus (25%), Actinomyces (30%) and Prevotella (10%). The rate of epidermoid cysts was 19.7% in histological examination. Age > 40 years, disease duration ≥12 years, the number of keloids >3 and the presence of family history are risk factors for keloid infections.
Gene expression responses of Bactrocera tryoni larvae feeding on different ripening stages of tomato fruit
Authors: Christensen (née Roohigohar) Shirin , van der Burg Chloé A. , Clarke Anthony R. , Prentis Peter J. 
Published: February 2023

AbstractThe Queensland fruit fly, Bactrocera tryoni, is an economically important pest insect of horticultural crops in Australia, damaging a wide variety of fruits and vegetables. To study the B. tryoni–fruit interaction, we conducted a transcriptomic investigation of B. tryoni larvae feeding on three ripening stages of tomato fruit, which had been previously shown to influence larval performance. Fruit fly larval survival in immature tomato fruit is very poor, which we hypothesized to be due to the effect of tomato fruit toxins which are mostly expressed in green fruit. We found 2709 transcripts differentially expressed in larvae feeding on the three ripening stages. The transcript expression pattern was most divergent between larvae feeding in immature fruit compared with fully ripe fruit, with intermediate expression in larvae feeding in colour‐break fruit. Gene Set Enrichment Analysis showed that larvae fed in immature fruit had a higher number of depleted GO terms in comparison with larvae fed on fully ripe fruit, particularly GO terms associated with larval development, metabolism and basic cellular functions. Enriched GO terms in larvae fed on fully ripe fruit showed the opposite pattern, with a greater number of enriched terms related to larval growth and detoxification processes. We concluded that poor larval survival in immature‐green fruit is likely because the substrate offers a very poor diet for larvae, which limits normal developmental functions. By contrast, in colour‐break and fully ripe fruit the substrate allows normal development, including the expression of metabolically costly detoxification mechanisms.
The role of ectopic P granules protein 5 homolog (EPG5) in DHPG‐induced pain sensitization in mice
Authors: Mei Xiangyang , Yin Chengyu , Pan Yushuang , Chen Lei , Wu Cheng , Li Xiangyao , Feng Zhiying 
Published: February 2023

AbstractNociplastic pain is a severe health problem, while its mechanisms are still unclear. (R, S)‐3,5‐Dihydroxyphenylglycine (DHPG) is a group I metabotropic glutamate receptor (mGluR) agonist that can cause central sensitization, which plays a role in nociplastic pain. In this study, after intrathecal injection of 25 nmol DHPG for three consecutive days, whole proteins were extracted from the L4~6 lumbar spinal cord of mice 2 h after intrathecal administration on the third day for proteomics analysis. Based on the results, 15 down‐regulated and 20 up‐regulated proteins were identified in mice. Real‐time quantitative PCR (RT‐qPCR) and western blotting (WB) revealed that the expression of ectopic P granules protein 5 homolog (EPG5) mRNA and protein were significantly up‐regulated compared with the control group, which was consistent with the proteomics results. Originally identified in the genetic screening of Caenorhabditis elegans, EPG5 is mainly involved in regulating autophagy in the body, and in our study, it was mainly expressed in spinal neurons, as revealed by immunohistochemistry staining. After the intrathecal injection of 8 μL adeno‐associated virus (AAV)‐EPG5 short hairpin RNA (shRNA) to knock down spinal EPG5, the hyperalgesia caused by DHPG was relieved. Altogether, these results suggest that EPG5 plays an important role in DHPG‐induced pain sensitization in mice.image
Barth's Repetition*
Authors: Stanley Timothy 
Published: February 2023

AbstractIn chapter three of volume 1.2 (§19‐21) of the Church Dogmatics, Karl Barth provided one of his most thoroughgoing accounts of the concept of scripture. Throughout, he held in tension the Word of God with the frailty of the Bible's human words. As Barth explored this two‐fold aspect of the Bible, he relied upon the concept of repetition. However, what has not been fully appreciated is how repetition was at work not just in Barth's account of the Word and letter of the text, but also the Bible's book form. In response, the following essay concentrates upon these three aspects of repetition in Barth's thought. First, it evaluates trinitarian repetition and circular imagery throughout the Church Dogmatics. Second, it clarifies how repetition featured in Barth's hermeneutics of the interior letter of the text. Third, it demonstrates how despite Barth's ambiguities on these matters, repetition also included the physical book. The result reframes Barth's relevance to the material study of the Bible, which when viewed in this light can inform critical debate about the openness of technological information cultures today.
Genetic diversity and phylogenetic relationships of apple chlorotic leaf spot virus isolates from Malus, Pyrus and Prunus hosts in Latvia
Authors: Zuļģe Neda , Gospodaryk Alina , Moročko–Bičevska Inga 
Published: February 2023

AbstractThe prevalence of apple chlorotic leaf spot virus (ACLSV) in different Rosaceae species, mainly from cultivated apple (Malus domestica), pear (Pyrus communis), plum (Prunus domestica), apricot (Prunus armeniaca) and wild plants of genus Amelanchier, Cotoneaster, Crataegus, Malus, Prunus and Sorbus were studied. ACLSV was found in cultivated M. domestica, P. communis, P. domestica and wild‐growing Malus, including M. sylvestris. Thirty‐four sequences of the ACLSV coat protein gene were obtained and compared with 372 homologous sequences from GenBank to evaluate the genetic diversity of the virus in Latvia and worldwide. The sequence identities varied from 79.6% to 98.4% at the nucleotide level and 84.5% to 100% at the amino acid level. Phylogenetic analyses clustered ACLSV pome fruit isolates into three genetic types but isolates from stone fruits into four phylogenetic groups. Three genetic types of ACLSV pome and stone fruit isolates were identified in Latvia. Two of them were previously characterized as type B6 and P205. The third genetic type was newly recognized among the stone fruit isolates and distinguished from other virus isolates by amino acid substitutions in three positions, Ser14, Asn23 and Leu72. Two natural recombinants, LV27 and NZ5, were detected among ACLSV isolates from Latvia, and recombination events were identified for the first time in wild‐growing Malus, confirming that recombination is a common process in the ACLSV population.
Hospital inpatient suicides: A retrospective comparison between psychiatric and non‐psychiatric inpatients in Milan healthcare facilities
Authors: Rucco Daniele , Gentile Guendalina , Tambuzzi Stefano , Fanton Beatrice , Calati Raffaella , Zoja Riccardo 
Published: February 2023

AbstractIntroductionInpatient suicide in hospitals is a worrying phenomenon that has received little attention. This study retrospectively explored the socio‐demographic, clinical, and suicide‐related characteristics of hospital inpatient suicides in Milan, Italy, which were collected at the Institute of Forensic Medicine during a twenty‐eight‐year period (1993–2020). In particular, this study compared the features of hospital inpatient suicides in patients with and without psychiatric diagnoses.MethodsData were collected through the historical archive, annual registers, and autopsy reports, in certified copies of the originals deposited with the prosecutors of the courts.ResultsConsidering the global sample, inpatients were mainly men (N = 128; 64.6%), with a mean age of 56.7 years (SD ± 19.8), of Italian nationality (N = 176; 88.9%), admitted to non‐psychiatric wards (N = 132; 66.7%), with a single illness (N = 111; 56.1%), treated with psychotropic medications (N = 101; 51%), who used violent suicide methods (N = 177; 89.4%), died of organic injuries (N = 156; 78.8%), and outside the buildings (N = 114; 72.7%). Comparing psychiatric and non‐psychiatric inpatients, suicide cases with a non‐psychiatric diagnosis were predominantly men (N = 48; 76.2%), hospitalized in non‐psychiatric wards (N = 62; 98.4%), assuming non‐psychotropic drugs (N = 37; 58.7%), and died in outside hospital spaces (N = 54; 85.7%).ConclusionsA fuller characterization of suicide among hospitalized inpatients requires systematic and computerized data gathering that provides for specific information. Indeed, this could be valuable for inpatient suicide prevention strategies as well as institutional policies.
Antibody, cell‐mediated response and infection susceptibility in allogeneic hematopoietic stem cell recipients after COVID‐19 mRNA vaccination
Authors: Pizzano Umberto , Facchin Gabriele , Marcon Chiara , Fabris Martina , Battista Marta Lisa , Cerno Michela , Geromin Antonella , Pucillo Martina , Petruzzellis Giuseppe , Vianello Giampaolo , Battaglia Giulia , Peressutti Roberto , Grillone Lucrezia , Tascini Carlo , Curcio Francesco , Fanin Renato , Patriarca Francesca 
Published: February 2023

AbstractBackgroundPatients undergoing allogeneic stem‐cell transplantation (allo‐SCT) have reduced responses to vaccines due to immunosuppressive status linked to GvHD prophylaxis and treatment. In our study, we compared humoral responses to anti‐SARS‐CoV‐2 mRNA vaccine, and infection onset, according to patients and transplant features; we also evaluated cellular response in patients without seroconversion.MethodsWe tested antibodies titer after second and third vaccine doses. Antibodies were detected through an immune‐enzymatic assay. In a patients’ subgroup without seroconversion, we tested cell‐mediated responses evaluating interferon‐gamma release by T‐lymphocytes exposed to virus spike protein.ResultsSeroconversion rate increased from 66% at 30 days to 81% at 90 days after the second dose; it was 97% at 150 days after the third dose. We found a significant association between seroconversion after the second dose and two variables: shorter interval between allo‐SCT and vaccination; ongoing immunosuppression. Twelve of 19 patients (63%) without antibodies after the second dose did not show cellular responses. Nineteen percent of patients developed SARS‐CoV‐2 infection after the third dose, with favorable outcome in all cases. Patients within 12 months after allo‐SCT showed a significantly higher infection risk.ConclusionsOur study suggests that an interval shorter than 12 months between allo‐SCT and first vaccine dose and/or ongoing immunosuppression were associated with humoral and cellular response deficiency after two doses. Third dose induced an increased and sustained humoral response in the majority of patients. However, patients within 1 year after allo‐SCT remained at higher infection risk and may be candidate for prophylaxis with anti‐SARS‐CoV‐2 monoclonal antibodies.
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Perception of blood donation among employees of healthcare organizations during COVID‐19 pandemic: A national multicenter cross‐sectional study
Authors: Al Zadjali Lubna , Batarfi Khalid , Badawi Maha , Elgohary Ghada , Aljabry Mansour , Alsuhaibani Omar , Ezzat Hatoon , Radhwi Ghazala , AlHashmi Hani 
Published: February 2023

AbstractBackgroundMaintaining a safe and adequate blood supply during a crisis is a major challenge facing blood banks around the world. With the recent global COVID‐19 crisis and the enforced “stay at home” lockdown, access to blood donors was limited. Since employees of healthcare facilities may act as potential blood donors, their perception of blood donation and their willingness to donate during the pandemic period is important to be assessed.Study Design and MethodA national cross‐sectional study at six centers in Saudi Arabia was conducted using an online‐based questionnaire that was distributed to all healthcare employees in these facilities between June and August 2020.ResultsAmong the total of 1664 participants, 63.2% (n = 1051) did not donate blood during the last 2 years. However, 53% (n = 882) of participants reported they are likely to donate blood during the COVID‐19 crisis. Furthermore, 85% (n = 1424) did not donate blood during the current pandemic, with the biggest worries of getting the COVID‐19 infection in the donor center. The main concerns of participants were about adherence to physical distancing requirements and the safety of the donation procedure. The majority of health care participants (88.2%) support implementing a hospital policy for a voluntary blood donation by employees during crises.ConclusionRecruitment of more blood donors among health care employees is a feasible solution to improve the blood supply during a crisis. This should be based on efforts throughout the year including regular awareness campaigns and effective communication.