bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a putative mycovirus library derived from Korean entomopathogenic fungi
Authors: Hwang Dong Young , Kim Seulki , Woo Soo Dong , Shin TaeYoung , Coutts Robert H. A. , Kotta-Loizou Ioly 
Published: January 2023

Abstract
Entomopathogenic fungi have potential as biocontrol agents against insect pests and mycovirus-mediated hypervirulence may enhance their efficacy. Before initiating research on hypervirulence, the presence or absence of double-stranded (ds) RNA elements has been determined in 94 Korean entomopathogenic fungi. DsRNA elements varying in size from ca. 0.8 to 7 kbp were found in 14.9% (14/94) of the strains examined, including Beauveria bassiana, Metarhizium pemphigi, Metarhizium pinghaense, Metarhizium rileyi and Cordyceps fumosorosea. This study provides information on the incidence and electrophoretic banding pattern of dsRNA elements and is the first report of mycoviruses in Korean entomopathogenic fungi.
Beating the Heat: Solution Sets for Developed Cities
Authors: Zhao Yongling , Sen Sushobhan , Susca Tiziana , Iaria Jacopo , Kubilay Aytaç , Gunawarden Kanchane , Zhou Xiaohai , Takane Yuya , Park Yujin , Wang Xiaolin , Rubin Andreas , Fan Yifan , Yuan Chao , Bardhan Ronita , Derome Dominique , Ürge-Vorsatz Diana , Carmeliet Jan 
Published: January 2023

Abstract
Beating excessive urban heat calls for a whole-system approach, where several individual mitigation measures have to be implemented together. We assess the use of green walls, green roofs, reflective roofs, thermally efficient buildings, high-efficiency indoor cooling, urban forestry, evaporative pavement, and constructed shade for directly reducing urban heat or indirectly reducing emissions of anthropogenic heat. An ITE-index is proposed to evaluate investment (I), time for implementation (T), and effectiveness (E) of 247 multi-measure solution sets and the 8 individual measures. Solution sets comprising 4-7 different measures are found to have a higher index score and to be more effective for different types of local climate. The ITE-index is a universally useful tool for decision-making for individual cities.
A systematic review on the association between ovarian and prostate cancer with <I>BRCA1</I> and <I>BRCA2</I> gene
Authors: Chitra Veena Sarpparajan , Vajagathali Mohammed , Ramakrishnan Veerabathiran 
Published: January 2023

Background. BRCA1 and BRCA2 were discussed as the basis of inherited adenocarcinoma and breast and ovarian malignancy. Ovarian cancer is uncommon in women below 40 years of age, and prostate cancer mainly occurs in older men cause 90 % in those above sixty-fve.Objective. The main objective of this paper is to investigate the relationship between ovarian and prostate cancer with the BRCA1 and BRCA2 genes.Material and Methods. The ovarian and prostate cancer mechanism is discussed in detail, and their preventive measures with screening techniques are also demonstrated. This systematic review collected the related articles from online databases using the key terms ovarian cancer, prostate cancer, BRCA genes, mutation, polymorphism, carcinoma, sarcoma, and genetic association.Results. Based on the obtained information, it is found that the BRCA genes are highly associated with prostate cancer in men, and in women, it is significantly linked with breast cancer than ovarian cancer.Conclusion. Therefore, early diagnosis and genetic testing for BRCA1&BRCA2 genes in both men and women are necessary. In some cases, these genes might even cause different types of cancer like pancreatic cancers. Identifying individuals with tumour-HRD through mutations in the homologous repair pathway and determining this gene expression is essential to improve treatment techniques developed during the previous decade and rapidly make their way into clinical trials practice. However, the safe introduction of these medicines into everyday practice will require a thorough understanding of treatment targets and associated adverse effects.
Optimization of mammosphere formation assay for quantif cation of il6-induced stemness in differentiated breast cancer cells
Authors: Nevskaya K. V. , Efimova L. V. , Korda P. V. , Dolgasheva D. S. , Ibragimova M. K. , Menyailo M. E. , Udut E. V. , Litviakov N. V. , Pershina A. G. 
Published: January 2023

The aim of the study was mammosphere assay optimization for quantifcation of IL6-induced stemness in differentiated (СD44– ) T47D breast cancer cells.Material and Methods. The effect of three commonly used cell-detaching methods (TrypLE, accutase, cell scrapper) at various confuence (40–50 % and 70–80 %) on cell viability, phenotypic profle and mammosphere formation was tested. The cell viability was examined using AnnexinV/propidium iodide assay. The phenotypic profle was analyzed by fow cytometry with fuorescent markers CD24 and CD44.Results. Detachment of the cells using scrapper led to substantial increase in early apoptotic and late apoptotic cells in comparison with TrypLE and accutase. Dissociation with TrypLE reduced the percentage of detected CD44+ positive cells, whereas accutase saved the surface marker. The number of mammosphere and their diameter did not differ between groups. Incubation of differentiated (CD44– CD24+) T47D cells with IL-6 for 24 hours resulted in an appearance of CD44+CD24+ and CD44+CD24–/low subpopulation. Furthermore, the differentiated cells after 24 hours of IL6 exposure formed 3 times more mammospheres compared to the control.Conclusion. Usage of cells with confuence of no more than 80 % and accutase for detachment of cells is recommended for mammosphere assay. Incubation of CD44– CD24+ T47D cells with IL6 for 24 hours is suffcient for stimulation of stemness plasticity.
Curcumin as an anti-proliferative agent in breast cancer through RassF1a, Bax, and caspase-3 protein
Authors: Rahmah N. A. , Harliansyah H. , Suyatna F. D. , Kanoko M. , Rustamadji P. , Prihartono J. , Bustami A. , Haryono S. J. , Hernowo B. S. 
Published: January 2023

Background. Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specifc mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in various cell types.This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells.Material and Methods. Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELISA. Caspase-3 activity was used to determine apoptosis and was tested using fow cytometry.Results. The results indicated that curcumin had a cytotoxicity effect of 40.85 µg/mL. At concentrations of 40 µg/mL and 50 µg/mL, curcumin increases levels of protein RASSF1A (∆ = 26.53% and 47.35%, respectively), Bax (∆ = 48.79% and 386.15%), and caspase-3 (∆ = 1,678.51% and 1,871.889%) signifcantly.Conclusions. Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax.
EVALUACIÓN DEL PROCESO DE TRANSFORMACIÓN DE LA ESCUELA EN COMUNIDADES PROFESIONALES DE APRENDIZAJE
Authors: Malpica Basurto Federico , Navareño Pinadero Pedro , Martínez Mesa Ruth Clarena 
Published: January 2023

La profesión docente ya no puede entenderse sin un trabajo colegiado que permita, a los profesionales, no solo ser buenos aplicadores de recetas individuales sino, sobre todo, sentirse seguros para desarrollar estrategias metodológicas comunes, probar nuevas prácticas colectivas y ser reconocido por ellas. Esta concepción supone la transformación de la cultura profesional docente imperante, para llegar a acuerdos constantes sobre lo que debe aprenderse en cada entidad educativa, y, en consecuencia, cuándo y cómo debe enseñarse y evaluarse lo aprendido, sobre los comportamientos de las personas, así como el tipo de experiencias institucionales para estimular el aprendizaje deseado.

Considerando a la entidad educativa como un sistema complejo, el modelo de transformación educativa positiva, en comunidades profesionales de aprendizaje (CPA), permite la intervención en todas estas dimensiones de manera conjunta, con el propósito de llevarla eventualmente a su punto de inflexión, mediante un enfoque altamente participativo de reflexión sobre la práctica y colaboración entre iguales, para generar estructuras de trabajo autónomas que sustenten la transformación a largo plazo. El trabajo de campo, realizado mediante la metodología de casos, y la posterior triangulación de la documentación de los proyectos, encuestas en línea y entrevistas, ha permitido concluir la importancia de la alineación de las prácticas docentes y directivas, y el grado de madurez de la organización educativa, en cuanto a las creencias de sus miembros, las variables técnico-pedagógicas, la cultura interna, el liderazgo, la comunicación entre los agentes educativos, así como la organización y gestión, en los procesos de transformación de educativa positiva.
LO NOVEDOSO Y LO PREOCUPANTE DEL ENFOQUE DE LA LICENCIATURA EN EDUCACIÓN PRIMARIA. PLAN 2022
Authors: Vidaña Dávila Cruz Eréndida , Rodríguez Esquivel David Uriel , Gaspar Velázquez Semiramis Armida , Bocanegra Salas Juan Antonio 
Published: January 2023

En el Sistema Educativo Mexicano se favorece un proceso de enseñanza-aprendizaje centrado en la transmisión de los conocimientos, a pesar de los discursos propuestos en los documentos oficiales, como el programa de estudio (SEP, 2022), los cuales parten de paradigmas distintos, como el socio-constructivismo. El texto intenta analizar críticamente el Plan de Estudios 2022, de la Licenciatura en Educación Primaria. La mirada teórica que orienta esta contribución se centra en el trabajo colaborativo e interdisciplinar, así como en la teoría sociocultural y el pensamiento crítico, que sustentan a dicho programa. El presente análisis retoma el paradigma de investigación interpretativo, desde un enfoque cualitativo, centrado en la metodología de análisis críticos del discurso.

En la lectura crítica del programa de estudios de la Licenciatura en Educación Primaria 2022, se identificaron las siguientes variables de análisis: participación de la comunidad en la educación; interdisciplinariedad y articulación de los contenidos; aprendizaje situado; pensamiento crítico, y educación inclusiva. Los resultados refieren a la importancia del vínculo entre la escuela y comunidad, el trabajo colaborativo entre docentes y el aprendizaje situado.

El Plan de Estudios 2022 resulta ser un proyecto curricular ambicioso que pretende transformar la educación básica a partir de la formación de un profesor que desarrolla capacidades y habilidades distintas a las habituales; sin embargo, convendría cuestionar si las políticas educativas implementadas hasta el momento, son suficientes para concretar esta propuesta en la realidad educativa de México, o si solo quedará como un proyecto de buenas intenciones.
Hybrid controller: main competences and skills
Authors: Cabral Pedro Henrique Diehl , Carraro Wendy Beatriz Witt Haddad 
Published: January 2023

Purpose: To identify in the scientific literature the main technical competences and professional skills associated with the technical bean counter and managerial business partner controller profiles.
Methodology: A literature review was conducted, indexed to the Web of Science and Scopus databases, using the Chartered Association of Business Schools 2018 ranking to select scientific articles published in high-quality journals in the last 10 years. The process resulted in a sample of 11 articles, on which content analysis was applied. The analysis was guided by 30 categories based on the consulted literature (theory-driven categorization). 558 codes were collected, and through them, the main competencies and skills needed by the hybrid controller were identified.
Results: The technical profile presents as its main competence the financial accounting and reporting competence, which concentrated more than 50% of the profile codes. The managerial profile presents managerial accounting competence and the interpersonal and communication skills as the main ones, together comprising less than 50% of the profile codes. The managerial profile identified is close to the hybrid controller profile, little explored in the literature, which fluctuates between the technical and managerial polar profiles due to contextual and cultural factors.
Contributions of the Study: The study contributes to academia by deepening discussions on the hybrid profile, pointing out their main competencies and skills, and identifying which contextual and cultural factors influence the professional profile. By highlighting the main competencies and skills, the study contributes to practice, which can be supported by these findings to better conduct selection processes, for example, in addition to highlighting the necessary alignment between organizational context and culture and the desired profile for the controller.
Synthesis, Drug-Likeness Evaluation of Some Heterocyclic Moieties Fused
Indole Derivatives as Potential Antioxidants
Authors: Kumari Archana , Singh Rajesh Kumar 
Published: January 2023

Background:
Indole and its derivatives have a wide range of pharmacological effects,
including analgesic, antimicrobial, antidepressant, anti-diabetic, anti-convulsant, anti-helminthic,
and anti-inflammatory properties. They are crucial structural components of many of today's powerful
antioxidant medications.

Objective:
Using the Schotten–Baumann reaction, the indole ring was linked to other key heterocyclic
moieties such as morpholine, imidazole, piperidine, and piperazine at the active 3rd position
and then tested for antioxidant activity.

Methods:
Synthesis of derivatives was accomplished under appropriate conditions and characterized
by IR, NMR (1H and 13C), and mass spectrum. Using the Swiss ADME online application,
ADME properties were also determined. The in vitro antioxidant activity was measured using
DPPH and Reducing power method.

Results:
In the DPPH assay, compounds 5a (IC50=1.01±0.22 μg/mL), 5k (IC50=1.21±0.07μg/mL),
whereas compounds 5a (EC50=23±1.00 μg/mL), 5h (EC50=26±2.42 μg/mL) in the reducing power assay
were most potent as compared with standard Ascorbic acid. Compounds 5a, 5h, and 5k demonstrated
maximal potency equivalent to standard. Lipinski's rule was followed in ADME outcomes.

Conclusion:
The synthesis and evaluation of indole derivatives to investigate their antioxidant action
has received a lot of attention. These discoveries could lead to more effective antioxidant candidates
being designed and developed.
Comparison of Sub-epithelial Connective Tissue Graft and Platelet Rich Fibrin in Peri-implant Soft Tissue Augmentation: A Randomized Clinical Split-mouth Study
Authors: Elkashty Ayman Abdel-Rahim , Aboelmaaty Wael Mohamed , Helmy Samah Mohammed , Elewa Mohammed Elsaed , Mansour Alaa , Ateia Islam Mohammed 
Published: January 2023

Aims and Background:
Gingival phenotype has a crucial impact on the peri-implant marginal bone stability. The aim of this clinical trial is to assess and compare the efficacy of the sub-epithelial connective tissue graft (SCTG) and platelet-rich fibrin (PRF) in improving the peri-implant soft tissue phenotype and enhancing esthetic outcomes.

Materials and Methods:
The present study was a split-mouth randomized controlled clinical trial. A total of ten patients who had bilateral missing teeth in the maxillary esthetic zone with a thin gingival phenotype were included in this study. For each study participant, one randomly selected site was treated with SCTG, while the other was treated with PRF membrane during dental implant placement. Treatment outcomes included the assessment of the facial gingival thickness using cone-beam computed tomography (CBCT) at the baseline (T0) and 6 months postoperatively (T1), and the Pink esthetic score (PES) at T1 and 3 months later after prosthesis placement (T2).

Results and Discussion:
Both treatment options resulted in a significant increase in gingival tissue thickness at T1 compared with T0, and in PES at T2 compared with T1 (p ˂ 0.05).

Conclusion:
PRF is an effective alternative to SCTG in augmenting peri-implant soft tissue phenotype and improving esthetic outcomes. This would help overcome the complications associated with harvesting the SCTG and increasing patients’ satisfaction.
Clinical Trial Registration ID: ISRCTN11961919.
Citric Acid Promoted Green Synthesis of Bioactive Heterocycles
Authors: Wagh Yogesh Baburao , Tayade Yogesh Ashok , Mahulikar Pramod Pandurang , Dalal Dipak Sharadrao 
Published: January 2023

Abstract:
In this review, we report green transformations of biologically active heterocycles catalyzed by
Citric acid. Citric acid is found naturally in citrus fruits, especially lemons and limes. Citric acid is soluble
in water used as a highly efficient and biodegradable catalyst for multi-component transformations, biomimetic
reactions, and C-C bond formation. It has been applicable for the multicomponent synthesis of
pyrano[2, 3-e]pyrimidin, pyrano[2, 3-d]pyrazol-amines, amidoalkyl naphthols, tetrahydropyridines, indazolo[
2,1-b]phthalazine-triones, indazolo[2,1-b]phthalazine-triones, indazolo[2,1-b]phthalazine-triones,
ethyl 3-methyl-4,5-dioxo-1,2-diphenylpyrrolidine-3-carboxylate and 2,4-disubstituted thiazoles from
ketones via C-Br, C-S, and C-N Bond Formations. Citric acid is also used in combination with ultrasound
for the synthesis of biologically active pyrazolyl-bis coumarinyl methanes and pyrrolidinone. Citric acid
is used in the generation of nanocatalysts, mesoporous carbon materials (OMCs) as well as polymerization
reactions by the reaction of resorcinol/formaldehyde resin. Citric acid is also used in the generation
of fluorescent 1,4-disubstituted-1,2,3-triazoles1,8-dioxo-decahydroacridines by Hantzsch condensation.
Carbon Sequestration of Fruit Trees under Contrasting Management Regimes
Authors: Wambede Muhamud Nabalegwa , Akello Gertrude , Mulabbi Andrew , Barasa Bernard , Lugumira Jerome Sebadduka , Amonya David 
Published: January 2023

This study aimed at establishing the potential of fruit trees in carbon sequestration. The specific objectives were to establish the carbon stocks in fruit trees compare the potential of carbon stocks in citrus and mango trees and examine the relationship between the management practices and carbon stocks in fruit trees. At the farm level, plots were identified and transects established and individual fruit trees from sampled individual farms along the transect were selected. At the tree level, measurements of tree height and diameter at breast height were made. They were converted to biomass using allometric equations. Analysis of Variance was used to compare the differences in carbon stocks between the fruit trees and between the different management practices. Findings revealed higher biomass and carbon stocks in mango trees as compared to citrus (74.57 ± 14.95 and 13.52 ± 1.25 t/ha respectively). Significant differences are also reported in carbon stocks under different management practices (p < 0.05). Irrespective of the species type, above-ground carbon under different management practices followed the order (from highest to lowest): Inorganic fertilizer < Intercrop < Monocrop < organic fertilizer and irrigation < intercrop and inorganic fertilizer. The results also point out that mango fruits have a high potential to sequestrate carbon emissions hence mitigating global warming.
Impurity Profiling: A Review
Authors: B Bhagwat Ashlesha , M Khedkar K. 
Published: January 2023

Impurities are any substances, such as starting materials or intermediates, that coexist with the parent drug or arise from side reactions. Interest in impurities present in APIs continues to grow. Nowadays, not only purity profile but also impurity profile is mandated by various regulatory agencies. Impurity profiling is a generic name for a group of analytical working groups such as describing, quantifying and characterizing identified and unidentified impurities present in a new drug substance. Impurity profiling is a novel approach aimed at detecting, identifying, structuring and quantifying organic and inorganic impurities and residual solvents in pharmaceuticals. Various regulatory bodies, such as ICH, USFDA, UKMHRA, and India's CDSCO, focus on impurity profiling and have formulated guidelines for impurity management and restriction. Impurities are classified into different categories based on their origin, type of composition, and biological safety. The presence of these unwanted chemicals or substances may affect the safety and effectiveness of the final medicinal product. This review focuses on the sources of impurities, their classification, and various analytical methods for the identification and quantification of impurities. Terms such as residual solvent, by-product, transformation product, decomposition product, reaction product, and related products are often used to define impurities.
Potential Interactions of Hypertension Drug in Medan City Pharmacy
Authors: Wahyuni Sri , Rawitri Kiki , Rahayu Yayuk Putri 
Published: January 2023

Background: Hypertension is a disease that can cause complications without the patient realizing it. Therapeutic management needed to control the patient's blood pressure is complex enough to potentially lead to drug interactions. The purpose of this study was to analyze the interaction of hypertension drugs that occurred in a pharmacy in the city of Medan.
Method: The study was conducted retrospectively involving 101 patient data in February-May 2022. Patient data was processed descriptively and potential drug interactions were analyzed using the Medscape Drug Interactions Checker, Stockley's Drug Interaction, and the drugs.com database
Result: Based on the study results, it was found that from 101 patients, 57 patients had no drug interactions (56.4%), but 45 cases of drug interactions were found in 44 patients (43.6%). Drug interactions found in 27 cases occurred pharmacokinetically (60%) and 18 cases occurred pharmacodynamically (40%). The most frequent drug interactions are Amlodipine and Simvastatin.
Conclusion: Based on this study, it can be concluded that most patients who have drug interactions occur through pharmacokinetic mechanisms.
Role of Vacha as Neurological and Metabolic Disorders: Evidence from Ethnopharmacology, Phytochemistry, Pharmacology and Preclinical Study
Authors: Roy Shibanjan Paul , Bundela Ragini , Jain Sourabh , Shukla Karunakar 
Published: January 2023

Vacha (Acorus calamus Linn (Acoraceae)) is a traditional Indian medicinal herb, which is practiced to treat a wide range of health ailments, including neurological, gastrointestinal, respiratory, metabolic, kidney, and liver disorders. The purpose of this paper is to provide a comprehensive up-to-date report on its ethnomedicinal use, phytochemistry, and pharmacotherapeutic potential, while identifying potential areas for further research. To date, 145 constituents have been isolated from this herb and identified, including phenylpropanoids, sesquiterpenoids, and monoterpenes. Compelling evidence is suggestive of the biopotential of its various extracts and active constituents in several metabolic and neurological disorders, such as anticonvulsant, antidepressant, antihypertensive, anti-inflammatory, immunomodulatory, neuroprotective, cardioprotective, and anti-obesity effects. The present extensive study is expected to provide insights into the involvement of several signaling pathways and oxidative mechanisms that can mitigate oxidative stress, and other indirect mechanisms modulated by active biomolecules of A. calamus to improve neurological and metabolic disorders.
Keywords: Acorus calamus, Acoraceae, Phytochemistry, Pharmacotherapeutic potential