bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of the induced effect of selected iron hydroxysulfates biosynthesized using Acidithiobacillus ferrooxidans for biomineralization of acid mine drainage
Authors: Wang Heru , Guo Qian , Guo Zehao , Luo Haowei , Li Honghu , Yang Jun , Song Yongwei 
Published: April 2023

Abstract
Soluble iron and sulfate in acid mine drainage (AMD) can be greatly removed through the formation of minerals facilitated by seed crystals. However, the difference in the effects of jarosite and schwertmannite as endogenous seed crystals to induce AMD mineralization remains unclear. This paper intends to study the effect of Fe2+ oxidation and Fe3+ mineralization in the biosynthesis of minerals using different addition amounts and methods of jarosite or schwertmannite. The results showed that the addition amount and method of different seed crystals had no effect on the Fe2+ bio-oxidation but would change the Fe3+ mineralization efficiency. With the same amount of seed crystals added, jarosite exhibited a higher capacity to promote Fe3+ mineralization than schwertmannite. Adding seed crystals before the initiation of Fe2+ oxidation (0 h) could significantly promote Fe3+ mineralization efficiency. With the increase of seed crystals, jarosite could not only shorten the time required for mineral synthesis but also improve the final mineral yield, whereas schwertmannite could only shorten the time required for mineral synthesis. When Fe2+ was completely oxidized to Fe3+ (48 h), the supplementary of jarosite could still effectively improve Fe3+ mineralization efficiency, but the addition of schwertmannite no longer affected the final mineralization degree.
Advanced treatment of secondary effluent by the integration of heterogeneous catalytic ozonation and biological aerated filter
Authors: Liu Xinghao , Yang Zhaoguang , Peng Jiayun , Chen Leilei , Yang Ying , Li Haipu , Yang Liqun 
Published: April 2023

Abstract
The advanced treatment of secondary effluents was investigated by employing heterogeneous catalytic ozonation integrated with a biological aerated filter (BAF) process. The results indicated that catalytic ozonation with the prepared catalyst (MnxCuyOz/γ-Fe2O3) significantly enhanced the performance of pollutant removal and broke up macromolecules into molecular substances by the generated hydroxyl radicals. These molecular substances were easily absorbed by microorganisms in the microbial membrane reactor. In the BAF process, chemical oxygen demand (COD) (chemical oxygen demand) decreased from 54.26 to 32.56 mg/L, while in catalytic ozonation coupled with the BAF, COD could be reduced to 14.65 mg/L (removal ratio 73%). Under the same condition, NH4+-N decreased from 77.43 to 22.69 mg/L and 15.73 mg/L (removal ratio 70%) in the BAF and the catalytic ozonation coupled with BAF, respectively. In addition, the model that highly correlated influent COD to effluent COD and reactor height for filler could predict the removal ratio of COD of the BAF system. Based on the microbial community analysis, ozone in the solution had a certain screening effect on microorganisms, which helped to better adapt to the ozone-containing environment. Therefore, the integrated process with its efficient, economic, and sustainable advantages was suitable for the advanced treatment of secondary effluents.
Resveratrol Attenuates Hydrogen Peroxide-induced Injury of Rat Ovarian
Granulosa-lutein Cells by Resisting Oxidative Stress via the SIRT1/Nrf2/ARE
Signaling Pathway
Authors: Cai Minghui , Wang Jiao , Sun Haijuan , Guo Qi , Zhang Chi , Yao Haixu , Zhao Chen , Jia Yuhan , Zhu Hui 
Published: April 2023

Introduction:
This paper aims to reveal the molecular mechanism of resveratrol against oxidative
stress and cell injury. The ovarian granulosa-lutein cell injury and apoptosis induced by oxidative stress may be
responsible for female luteal phase deficiency. The antioxidant function of resveratrol has been confirmed; however,
its effect on the expression of antioxidant enzymes and regulatory mechanisms in ovarian granulosa-lutein
cells remains unclear.

Objective:
This study aimed to investigate the role of the SIRT1/Nrf2/ARE signaling pathway in the effect of
resveratrol on the hydrogen peroxide-induced injury of rat ovarian granulosa-lutein cells.

Methods:
In this study, ovarian granulosa-lutein cells extracted from 3-week female SD rats were treated with
200 μM H2O2 in the presence or absence of 20 μM resveratrol. siRNA-SIRT1 and siRNA-Nrf2 were used to inhibit
the expression of SIRT1 and Nrf2, respectively. Cell counting kit 8 (CCK-8), cellular morphology, progesterone
secretion, and estradiol were used to evaluate cell injury. Hoechst 33258 staining was used to measure
cell apoptosis. DHE staining, DCFH-DA staining, malondialdehyde content, protein carbonyl content, total antioxidant
capacity and SOD viability were used to estimate the levels of oxidative stress. Western blot analysis
was used to detect the levels of apoptosis-related proteins, and SIRT1/Nrf2/ARE signaling pathway-related proteins.

Results:
The H2O2 treatment-induced rat ovarian granulosa-lutein cells injury was shown as decreased cell viability,
impaired cellular morphology, and decreased levels of progesterone and estradiol. The H2O2 treatment also
exacerbated cell apoptosis demonstrated as more apoptotic cells stained by Hoechst staining, decreased level
of anti-apoptosis protein Bcl-2 and increased level of pro-apoptosis protein Bax. These effects of cell injury and
apoptosis induced by H2O2 can be ameliorated by resveratrol. Resveratrol also alleviated oxidative stress induced
by H2O2, supported by decreased superoxide anion and cellular total ROS, decreased malondialdehyde
and protein carbonyl levels, and increased total antioxidant capacity and SOD viability. Western blot results demonstrated
resveratrol reversed the H2O2-induced decrease in levels of antioxidant enzymes containing ARE sequences
and activated SIRT1/Nrf2 pathway. Further treatment by siRNA-Nrf2 suggested resveratrol could not
activate the expression of antioxidant enzymes under a condition of inhibition of Nrf2.

Conclusion:
This study demonstrates that resveratrol attenuated oxidative stress to protect H2O2-induced rat
ovarian granulosa-lutein cell injury and apoptosis via SIRT1/Nrf2/ARE signaling pathway.
The Severity of Adverse Events is Associated with the Age of Hospitalized Patients: A Retrospective Study
Authors: Moya-Salazar Jeel , Añanca Graciela , Goicochea Eliane A. , Rojas-Zumaran Victor , Moya-Salazar Belén , Contreras-Pulache Hans 
Published: April 2023

Background:
Adverse events (AE) are unintentional injuries or harm that occur during medical care. These represent a public health problem, and they are one of the main causes of death in the world.

Objective:
The objective of the study was to determine AEs derived from nursing care in hospitalized patients, as well as to estimate their frequency according to severity, follow-up, and type of care service.

Methods:
We undertook a retrospective study in hospitalized patients. EA by severity (no damage, irreversible damage, disability, and death), types (pressure ulcers (PUs), falls, phlebitis, and blood transfusion), and demographic data (age and gender) were taken into account. Damage severity was determined according to the guidelines of the Peruvian Ministry of Health.

Results:
We obtained 127 reports (55.9% women, mean age of 28+8 years). The main EA were PUs (57.5%), followed by phlebitis (20.5%) and falls (19.7%). Most of the AEs have been followed up, and almost all the AEs occurred during hospitalization. In addition, being older was associated with AE severity (p=0.002), as there were reports of 2 (1.6%) cases of death, and half of the cases resulted in the disability of patients > 48 years old.

Conclusion:
PUs were the most frequent AE. They affected younger patients, and their severity was associated with older age. It is important for health professionals to recognize and notify AEs in order to ensure the patients’ well-being; also, health institutions needs to be encouraged for improving their risk reduction programs.
Herbal Medicines Used for the Management of COVID-19
Authors: Kumar Virender , Kumar Yogesh , Huria Rajat , Kumar Suryadev , Kalson Tarun , Jangra Deepak , Kumar Davinder , Mangla Bharti 
Published: April 2023

Abstract:
A worldwide outbreak of respiratory illnesses has been caused by coronavirus disease
(COVID-19). Traditional healers have used herbs and dietary plants for centuries to treat various conditions.
This review discusses the prevention of COVID-19, multiple herbs used in the treatment of
COVID-19, and their future perspectives. Various databases, such as PubMed, Web of Science, Scopus,
Medline, and Google Scholar, were searched for articles related to herbal products' antiviral effects using
different keywords: herbal, SARS-CoV-2, plant-derived drugs, COVID-19, coronavirus, etc. Herbal
treatment has been used as a contemporary alternative medicine for COVID-19. By inhibiting the replication
and entry of SARS-CoV-2 into host cells, herbs can inhibit the pathogenesis of COVID-19. This
article discusses COVID-19 infection, its salient features, spread, the life cycle of coronavirus, active
response to coronavirus, proposed treatment, and herbal drugs used in the prevention and treatment of
COVID-19.
ЗВ’ЯЗОК МІЖ ЗМІНАМИ КОНЦЕНТРАЦІЇ МАЛОНОВОГО ДІАЛЬДЕГІДУ ТА ПОКАЗНИКАМИ ЛІПІДНОГО СПЕКТРУ КРОВІ У ПАЦІЄНТІВ З СТАБІЛЬНОЮ ІШЕМІЧНОЮ ХВОРОБОЮ СЕРЦЯ ТА СУПУТНЬОЮ АРТЕРІАЛЬНОЮ ГІПЕРТЕНЗІЄЮ
Authors: Д. А. Волинський  , І. П. Вакалюк  
Published: April 2023

Ішемічна хвороба серця (ІХС) і супутня артеріальна гіпертензія (АГ) – ключові проблеми сучасної кардіології. В основі атерогенезу, який відіграє вирішальну роль в прогресуванні обох патологій, лежить оксидативний стрес і ендотеліальна дисфункція. Лабораторними маркерами для оцін- ки перебігу вказаних процесів є концентрація загального холестерину (ЗХ), ліпопротеїдів низької густини (ЛПНГ) та малонового діальдегіду (МА). Вивчення взаємозв’язків між змінами рівнів цих показників може стати перспективним напрямком в діагностиці ІХС та АГ і сприятиме покра- щенню і оптимізації медикаментозного лікування. Метою дослідження є виявити зв’язок між змінами концентрації МА та ЗХ і ЛПНГ у пацієнтів з стабільною ІХС з та без супутньої АГ. У дослідження включено 131 паці- єнтів із стабільною ІХС і АГ. Хворі були розділені на 2 групи: 1) 80 хворих із стабільною ІХС та супутньою АГ; 2) 51 хворих із стабільною ІХС без супутньої АГ. В ході дослідження ми оцінювали коливання рівнів МА. Ліпід- ний спектр крові оцінювався за рівнем в сироватці крові ЗХ, ЛПНГ. Рівень МА у групі пацієнтів із супутньою АГ достовірно вищий. Ми встановили слабкий прямий кореляційний зв’язок між МА і ЗХ, МА і ЛПНГ у групі хво- рих на ІХС із супутньою АГ. У пацієнтів без патологічних коливань арте- ріального тиску ми виявили слабкий зворотній кореляційний зв’язок. Нам вдалося встановити зв’язок між змінами концентрації МА та ЗХ і ЛПНГ у пацієнтів з стабільною ІХС з та без супутньої АГ, що підтверджує сут- тєвий вплив оксидативного стресу та ендотеліальної дисфункції, які є не- від’ємними супутниками АГ, на ліпідний спектр крові.
Ключові слова: ішемічна хвороба серця, артеріальна гіпертензія, окси- дативний стрес, малоновий діальдегід, ендотеліальна дисфункція.
PHYTOCHEMICAL ANALYSIS AND MOLECULAR CHARACTERIZATION OF MORINGA OLEIFERA AND ITS ENDOPHYTIC FUNGI
Authors: PRIYANKA G. DEVI , SHOBHA  , S. D. SHRUTHI 
Published: April 2023

Objective: Moringa oleifera Lam (Moringaceae) is a highly valued plant which has an impressive range of medicinal uses with high nutritional value. Different parts of the plant were being used for the treatment of illness due to the presence of various secondary metabolites that gives the plant anti-bacterial, anti-oxidant and other properties. These secondary metabolites are synthesized by endophytic organisms, which are present inside the tissues of plant.
Methods: ITS regions were amplified using a polymerase chain reaction for molecular identification. The sequences were analysed using BLAST n and DNA barcoding of plant was done for the identification of species. The organisms were refined on Potato Dextrose stock for identification of metabolites.
Results: It was found that fungi obtained are Aspergillus fumigatus and Tricoderma turrialbense with percentage identity of 96% and 98%, respectively, as obtained from NCBI BLAST; the metabolites were extracted with ethanol extract and Flavonoids and terpenoids are the main phytoconstituents present in endophytic fungi.
Conclusion: The results obtained support the medicinal properties of plant, which gets enhanced due to the presence of endophytic fungi present in them.
KULTUR JARINGAN KELAPA SAWIT
Authors: Rahma Pratiwi Dian , Wening Sri , Supena Nanang , Sediawati Retno Diah , Yenni Yurna 
Published: April 2023

Kelapa sawit merupakan tanaman perkebunan penghasil minyak yang penting di dunia. Salah satu upaya untuk memenuhi kebutuhan minyak sawit yang tinggi adalah dengan penggunaan bahan tanaman yang unggul, contohnya klon kelapa sawit unggul. Klon kelapa sawit unggul memiliki kelebihan dibandingkan bahan tanaman unggul hasil persilangan, yaitu sifatnya yang seragam dan produktivitas per hektar yang lebih tinggi. Pengembangan klon kelapa sawit memiliki beberapa kendala, di antaranya masalah abnormalitas dan efisiensi proses kultur jaringan. Hal ini memicu berkembangnya penelitian di bidang kultur jaringan kelapa sawit sebagai upaya untuk mengatasi permasalahan yang ada. Beberapa penelitian telah menunjukkan hasil yang menggembirakan. Meskipun mengalami beberapa kendala, kultur jaringan di PPKS memiliki peluang untuk mendapatkan bahan tanaman unggul kelapa sawit yang rendah abnormalitasnya serta proses kultur jaringan yang lebih efisien. Tulisan ini mengulas tantangan yang dihadapi dalam penerapan teknologi kultur jaringan kelapa sawit dan usaha-usaha pemecahannya, serta peluang pengembangan kultur jaringan kelapa sawit.
LEDAKAN HAMA MINOR ULAT API KECIL Olona gateri dan Penthocrates sp. (Lepidoptera: Limacodidae) DI PERKEBUNAN KELAPA SAWIT
Authors: Pradana Mahardika Gama , Priwiratama Hari , Rozziansha Tjut Ahmad Perdana , Purba Wiharti Oktaria , Susanto Agus 
Published: April 2023

Kegiatan budidaya kelapa sawit di Indonesia telah berlangsung selama lebih dari seratus tahun. Beberapa serangga mengalami proses adaptasi untuk dapat melakukan aktivitas makan dan meletakkan telur pada kelapa sawit sebagai tanaman inang. Akibat interaksi tersebut, terdapat berbagai jenis serangga yang bersifat sebagai hama baik mayor maupun minor. Pada waktu tertentu, populasi hama minor mengalami ledakan misalnya spesies O. gateri dan Penthocrates sp. Hal tersebut terjadi karena pengaruh beberapa faktor seperti dominasi hama utama yang bergeser akibat populasinya menurun. Penurunan populasi dapat terjadi karena berbagai pengaruh seperti terkendali oleh musuh alami, penggunaan insektisida, maupun faktor iklim yang tidak sesuai. Monitoring hama yang akurat dan kontinyu menjadi salah satu kunci sukses dalam mencegah ledakan hama minor maupun hama utama pada umumnya.
Kata kunci: Olona gateri, Penthocrates sp., resurgensi, dinamika populasi
Management of Multifactorial Stained Enamel Defects on Anterior Dentition Using a Combination Treatment of Whitening, Microabrasion, Resin Infiltration and Resin-based Composite Restoration
Authors: Alkhazaleh A , Tsujimoto A 
Published: April 2023

SUMMARY

Objectives
This case report describes a combination treatment composed of tooth whitening, microabrasion, resin infiltration, and resin-based composite restoration to manage multifactorial stained enamel lesions on anterior teeth.

Clinical Considerations
The patient’s primary concern was to improve the esthetics of their smile after about a decade of extensive orthodontic and orthognathic procedures. After clinical evaluation and history taking, the authors determined that the enamel defects were the result of carious and developmental factors. In-office tooth whitening took place in one visit, followed by micro abrasion and resin infiltration in another visit. Thirteen months later, a follow-up visit showed that the results were stable and that the patient was satisfied with the overall outcome. A small resin composite restoration was added at the follow up visit to repair a previous restoration and to reestablish the facial contours of the central incisor.

Conclusions
A combination treatment protocol composed of tooth whitening, microabrasion, and resin infiltration can improve patient satisfaction and avoid unnecessary invasive treatment.
Life cycle cost assessment and economic analysis of a decentralized wastewater treatment to achieve water sustainability within the framework of circular economy
Authors: López-Serrano María J. , Lakho Fida Hussain , Van Hulle Stijn W. H. , Batlles-delaFuente Ana 
Published: April 2023

Research background: The increasing water demand together with an unceasing production of wastewater worldwide has resulted in a situation where the scarcity and pollution of water resources are jeopardizing and depleting such a vital asset.
Purpose of the article: In this context, Nature Based Solutions (NBS) such as Vertical Flow Constructed Wetlands (VFCWs) are key because of their capacity of channelling a waste into a resource. However, and notwithstanding their essential role, their financial benefits too often go unnoticed because of missing research that study them from an economic perspective and this article has covered this existing gap. The objective of this research is to analyse the economic consequences of using VFCW against its traditional alternative through a comprehensive economic assessment.
Methods: After doing a Life Cycle Assessment (LCA), a combination of two approaches has been carried out. This research has developed a holistic approach where a Life Cycle Cost Assessment (LCCA) based on a Cost Benefit Analysis (CBA) along with an economic evaluation of cleaning environmental costs have been calculated for two different scenarios. For this monetary analysis, the environmental externalities derived from the use of cleaning the pollution caused by a public water supply and sewerage system and the VFCW have been quantified.
Findings & value added: Results conclude that VFCW apart of being a cost-effective and profitable alternative for an investor, it has also valuable benefits for the society in general because of its meaningful and positive externalities and the high removal cost of the environmental pollutants of the traditional water supply and sewage system both contributing directly to the achievement of Sustainable Development Goals (SDGs). Furthermore, 4/5 environmental impacts derived from the use of traditional alternative pollute more than twice as much as the VFCW does. Lastly, the cleaning costs difference between both alternatives is 1,984,335?.
A moderated mediation model of perceived barriers, entrepreneurial self-efficacy, intentions, and behaviors: A social cognitive career theory perspective
Authors: Duong Cong Doanh 
Published: April 2023

Research background: Although perceived barriers are considered one of the central constructs in entrepreneurship research, most previous studies only examine the direct effect of perceived barriers on attitudes and/or intentions to become entrepreneurs. Little attention is paid to how perceived barriers can weaken individuals? translation from entrepreneurial intentions to actual behaviors.
Purpose of the article: This research aims to adopt the Social Cognitive Career Theory and a moderated mediation model to bridge the entrepreneurial intention-action link, investigate the moderation effects of perceived barriers on this link and the mediation path from entrepreneurial self-efficacy to entrepreneurial behavior via entrepreneurial intention.
Methods: A valid sample of 1,698 Vietnamese respondents with real working and business experiences through the stratified random sampling with three stages and PROCESS macro approach have been used to examine the moderated mediation effect of perceived barriers on the entrepreneurial self-efficacy-intention-behavior linkages.
Findings & value added: The findings of this study shed new light on entrepreneurial literature by applying the Social Cognitive Career Theory to illustrate the moderated mediation effects of perceived barriers and entrepreneurial intention in the relationship between entrepreneurial self-efficacy and entrepreneurial behavior. Particularly, the translation from entrepreneurial intentions into start-up actions was found to became weaker when perceived barriers was high. Moreover, perceived barriers were also found to negatively moderate the indirect effects of entrepreneurial self-efficacy on start-up behaviors through entrepreneurial intentions. The findings of our study also provide several essential recommendations for policymakers and practitioners to encourage individuals? business venture creations and enhance entrepreneurial ecosystem.
Efecto de la eficacia de los microorganismos nativos y la composta en tres pisos altitudinales en el cultivo de quinua (Chenopodium quinoa) variedad INIA 415-Pasankalla
Authors: Galecio-Julca Miguel , Neira-Ojeda María , Chanduvi-García Roger , Peña-Castillo Ricardo , Álvarez-Bernaola Luis Armando , Granda-Wong Carlos , Lindo-Seminario David , Saavedra-Alberca Elizabeth , Javier-Alva Javier , Morales-Pizarro Davies Arturo 
Published: April 2023

Los abonos orgánicos son una alternativa sostenible con el agroecosistema frente a los fertilizantes convencionales causantes de la contaminación de los recursos naturales. El objetivo fue determinar la eficacia de los microorganismos eficientes nativos (MEN) y de compost sobre el rendimiento y rentabilidad en el cultivo de quinua variedad INIA 415-Pasankalla en tres pisos altitudinales (Faical 1935 m de altitud, Lagunas Amarillas 2328 m de altitud. y Cascapampa 2995 m de altitud). Se evaluaron cinco tratamientos: T0 (testigo), T1 (MEN 2.5%), T2 (MEN 5.0%), T3 (MEN 2.5%+0.9 kg compost m-1) y T4 (MEN 5.0%+1.8 kg compost m-1), evaluando: AP (altura de planta), RHa (rendimiento hectárea-1), RPa (rendimiento planta-1), PP (peso de panoja), PMG (peso de mil granos) y DT (diámetro de tallo). Además, se analizó el beneficio/costo por tratamiento. Se realizó un análisis de componentes principales ACP, una comparación triple de ANOVA y un análisis de correlación. Con el ACP se obtuvo dos componentes (C1 y C2) explicando el 97.20% de la variabilidad del estudio. El Componente 1 (78.46%) explica la mayor variabilidad y agrupó variables: G-I (AP, DT), G-II (RHa, Rpa, PP). Los tratamientos T4 y T3 presentaron los mejores resultados en relación a la altitud: Lagunas Amarillas-T4 con G-I y Cascapampa-T4 con G-II presentaron los mayores valores del estudio. No obstante, Cascapampa-T2 fue el más rentable (b/c= 5.68). La mezcla combinada de los insumos orgánicos elaborados a partir de materias primas locales y aplicados en forma foliar y suelos presentes en T4, mejoró los rendimientos y la rentabilidad del cultivo de quinua en relación a la altitud.
Vestir a cidade de festa
Authors: Pereira Alves de Sousa Patrício , Moreira de Souza Ingrid 
Published: April 2023

Este artigo debate como as cidades de Paraty (RJ) e Pirenópolis (GO) são trajadas pelas festas do Divino Espírito Santo, encaminhando um debate sobre como os corpos vestidos para a celebração e o conjunto de materialidades que ornamentam a festa revestem as referidas cidades patrimoniais. Nossa atenção se voltou para as vestimentas enquanto materialidades resultantes e geradoras de formas de sociabilidade que aparecem descritas e interpretadas nos dossiês de registro dos bens intangíveis do Instituto do Patrimônio Histórico e Artístico Nacional (IPHAN). A análise de conteúdo, procedimento metodológico adotado, dirigiu a pesquisa em resposta às seguintes perguntas: os Dossiês de Registro da Festa do Divino foram elaborados “para dizer o quê?” e “como disseram aquilo que se propuseram dizer?”. As problematizações realizadas permitem pensar, desse modo, como a festa veste a cidade patrimônio e como dois diferentes sentidos de materialidade convivem para o IPHAN e para as referidas cidades.