bluuebunny/crossref_metadata_2025_split huggingface dataset:
Randomized clinical trial on the clinical effects of a toothpaste containing extra virgin olive oil, xylitol, and betaine in gingivitis
Authors: Rodríguez-Agurto Alejandro , Bravo Manuel , Magán-Fernandez Antonio , López-Toruño Ana , Muñoz Ricardo , Ferrer Joaquín , Mesa Francisco 
Published: April 2023

AbstractTo determine the effects on gingival bleeding, dental biofilm, and salivary flow and pH in patients with gingivitis of using toothpaste with extra-virgin olive oil (EVOO), xylitol, and betaine in comparison to a placebo or commercial toothpaste. This controlled, double blinded, and multicenter randomized clinical trial included patients with gingivitis randomly assigned to one of three groups: test group (EVOO, xylitol, and betaine toothpaste), control group 1 (placebo toothpaste), or control group 2 (commercial toothpaste). Percentage supragingival biofilm and gingival bleeding were evaluated at baseline (T0), 2 months (T2), and 4 months (T4), measuring non-stimulated salivary flow and salivary pH. Comparisons were performed between and within groups. The final study sample comprised 20 in the test group, 21 in control group 1, and 20 in control group 2. In comparison to control group 1, the test group showed significantly greater decreases in gingival bleeding between T4 and T0 (p = 0.02) and in biofilm between T2 and T0 (p = 0.02) and between T4 and T0 (p = 0.01). In the test group, salivary flow significantly increased between T2 and T0 (p = 0.01), while pH alkalization was significantly greater between T4 and T0 versus control group 2 (p = 0.01) and close-to-significantly greater versus control group 1 (p = 0.06). The toothpaste with EVOO, xylitol, and betaine obtained the best outcomes in patients with gingivitis, who showed reductions in gingival bleeding and supragingival biofilm and an increase in pH at 4 months in comparison to a commercial toothpaste.
Effects of fine grinding on mid-infrared spectroscopic analysis of plant leaf nutrient content
Authors: Whatley Caleb R. , Wijewardane Nuwan K. , Bheemanahalli Raju , Reddy K. Raja , Lu Yuzhen 
Published: April 2023

AbstractFourier transform mid infrared (FT-MIR) spectroscopy combined with modeling techniques has been studied as a useful tool for multivariate chemical analysis in agricultural research. A drawback of this method is the sample preparation requirement, in which samples must be dried and fine ground for accurate model calibrations. For research involving large sample sets, this may dramatically increase the time and cost of analysis. This study investigates the effect of fine grinding on model performance using leaf tissue from a variety of crop species. Dried leaf samples (N = 300) from various environmental conditions were obtained with data on 11 nutrients measured using chemical methods. The samples were scanned with attenuated total reflectance (ATR) and diffuse reflectance (DRIFT) FT-MIR techniques. Scanning was repeated after fine grinding for 2, 5, and 10 min. The spectra were analyzed for the 11 nutrients using partial least squares regression with a 75%/25% split for calibration and validation and repeated for 50 iterations. All analytes except for boron, iron, and zinc were well-modeled (average R2 > 0.7), with higher R2values on ATR spectra. The 5 min level of fine grinding was found to be most optimal considering overall model performance and sample preparation time.
Bioinformatics analysis of the pathogenic link between Epstein-Barr virus infection, systemic lupus erythematosus and diffuse large B cell lymphoma
Authors: Zhu Qian-Ying 
Published: April 2023

AbstractEpstein-Barr virus (EBV) is a risk factor for diffuse large B-cell lymphoma (DLBCL) and systemic lupus erythematosus (SLE). While prior research has suggested a potential correlation between SLE and DLBCL, the molecular mechanisms remain unclear. The present study aimed to explore the contribution of EBV infection to the pathogenesis of DLBCL in the individuals with SLE using bioinformatics approaches. The Gene Expression Omnibus database was used to compile the gene expression profiles of EBV-infected B cells (GSE49628), SLE (GSE61635), and DLBCL (GSE32018). Altogether, 72 shared common differentially expressed genes (DEGs) were extracted and enrichment analysis of the shared genes showed that p53 signaling pathway was a common feature of the pathophysiology. Six hub genes were selected using protein–protein interaction (PPI) network analysis, including CDK1, KIF23, NEK2, TOP2A, NEIL3 and DEPDC1, which showed preferable diagnostic values for SLE and DLBCL and involved in immune cell infiltration and immune responses regulation. Finally, TF-gene and miRNA-gene regulatory networks and 10 potential drugs molecule were predicted. Our study revealed the potential molecular mechanisms by which EBV infection contribute to the susceptibility of DLBCL in SLE patients for the first time and identified future biomarkers and therapeutic targets for SLE and DLBCL.
Nationwide sample data analysis of additional surgery rate after anterior or posterior cervical spinal surgery
Authors: Yuh Woon Tak , Kim Minjung , Choi Yunhee , Han Junghoon , Kim Junhoe , Kim Taeshin , Chung Chun Kee , Lee Chang-Hyun , Park Sung Bae , Kim Kyoung-Tae , Rhee John M. , Park Moon Soo , Kim Chi Heon 
Published: April 2023

AbstractSurgical outcomes of degenerative cervical spinal disease are dependent on the selection of surgical techniques. Although a standardized decision cannot be made in an actual clinical setting, continued education is provided to standardize the medical practice among surgeons. Therefore, it is necessary to supervise and regularly update overall surgical outcomes. This study aimed to compare the rate of additional surgery between anterior and posterior surgeries for degenerative cervical spinal disease using the National Health Insurance Service-National Sample Cohort (NHIS-NSC) nationwide patient database. The NHIS-NSC is a population-based cohort with about a million participants. This retrospective cohort study included 741 adult patients (> 18 years) who underwent their first cervical spinal surgery for degenerative cervical spinal disease. The median follow-up period was 7.3 years. An event was defined as the registration of any type of cervical spinal surgery during the follow-up period. Event-free survival analysis was used for outcome analysis, and the following factors were used as covariates for adjustment: location of disease, sex, age, type of insurance, disability, type of hospital, Charles comorbidity Index, and osteoporosis. Anterior cervical surgery was selected for 75.0% of the patients, and posterior cervical surgery for the remaining 25.0%. Cervical radiculopathy due to foraminal stenosis, hard disc, or soft disc was the primary diagnosis in 78.0% of the patients, and central spinal stenosis was the primary diagnosis in 22.0% of them. Additional surgery was performed for 5.0% of the patients after anterior cervical surgery and 6.5% of the patients after posterior cervical surgery (adjusted subhazard ratio, 0.83; 95% confidence interval, 0.40–1.74). The rates of additional surgery were not different between anterior and posterior cervical surgeries. The results would be helpful in evaluating current practice as a whole and adjusting the health insurance policy.
Systemic effects of oral tolerance in bone healing
Authors: Costa Bruno Henrique , Rezende Alisson Kennedy , Costa Lais , Neves Gabrielle Fernanda Monteiro , Shimano Antônio Carlos , de Oliveira Penoni Álvaro , Carvalho Claudia Rocha , Costa Raquel Alves , de Alvarenga Erika Costa 
Published: April 2023

AbstractBone fractures cause acute inflammation that, despite being important for initial repair, may delay the healing of the damaged bone. Parenteral injection of dietary protein has been shown to decrease inflammation and accelerate the repair of skin wounds and other inflammatory pathologies. Thus, our aim was to evaluate whether the intraperitoneal (i.p.) immunization with zein, an abundant protein in rodent chow, would favor bone healing. Wistar rats received i.p. immunization: saline (SG), adjuvant (AG) and zein associated with adjuvant (ZG). Then, a 2 mm of defect bone was performed on the right tibia, and on days 7, 14, 28 and 45 thereafter, analyses were performed. The results showed that the injection of zein reduced inflammation without impairing bone mineralization. Moreover, biomechanical tests demonstrated higher levels of maximum force (N) in ZG, indicating better mechanical resistance in relation to the others. The computerized tomography also indicated lower levels of medullary content in the ZG than in the SG, suggesting the absence of trabeculae in the medullary region in the ZG. These findings suggest that the injection of zein in previously tolerated animals may improve bone repair, leading to mechanically functional bone formation.
Molecular mechanism of green tea polyphenol epicatechin gallate attenuating Staphylococcus aureus pathogenicity by targeting Ser/Thr phosphatase Stp1
Authors: Gao Yawen , Wang Hongsu , Li Xuening , Niu Xiaodi 
Published: April 2023

In this study, we discovered that ECG, a polyphenol compound extracted from green tea, demonstrated marked anti-Ser/Thr phosphatase (Stp1) activity towards Staphylococcus aureus (S. aureus) with an IC50 value of 8.35 μM.
Brainprint based on functional connectivity and asymmetry indices of brain regions: A case study of biometric person identification with non‐expensive electroencephalogram headsets
Authors: Ortega‐Rodríguez Jordan , Martín‐Chinea Kevin , Gómez‐González José Francisco , Pereda Ernesto 
Published: April 2023

AbstractBrain‐computer interface applications for biometric person identification have increased their interest in recent years since they are potentially more secure and more difficult to counterfeit than traditional biometric techniques. However, it is necessary to consider how brain waves are acquired for this purpose, not only in terms of efficiency but also of practical comfort for the user and the affordability degree of the biosignal acquisition device so that their everyday application can become a realistic possibility. In this context, this paper presents the capabilities of using a non‐expensive wireless electroencephalogram (EEG) device to extract spectral‐related and functional connectivity information of brain activity. The proposed method achieved a sufficient biometric identification with two datasets of 13 and 109 subjects when comparing the performance of a sizeable classification algorithm set. In addition, a novel feature in EEG biometric identification, called asymmetry index, is introduced here. Furthermore, this is the first study in this field to consider the effect of the time‐lapse between different recording sessions on the system's behaviour when using a low‐cost EEG device with identification accuracy rates of up to 100%.
Analysis method of voltage quality in distribution station area based on high‐speed power line carrier and GA‐ANN in smart grid
Authors: Deng Hanjun , Yang Shuai , Huang Rui , Liu Mouhai , Ma Yeqin , Xie Yinghai 
Published: April 2023

AbstractAiming at the problem that most of the existing methods cannot obtain and accurately analyse the voltage quality of distribution network in time, a method for monitoring and analysing the voltage quality of distribution station area based on high‐speed power line carrier under the power Internet of Things (PIoT) architecture in smart grid is proposed. Firstly, based on the PIoT architecture, a monitoring system for the distribution station area is designed, which realises the reliable monitoring of the voltage in the station area through the information interaction of the sensing layer, the network layer, the platform layer and the application layer. Then, the high‐speed power line carrier is used to realise the rapid monitoring and collection of the station data, which improves the quality of basic data, and the principal component analysis method is used to extract the voltage quality characteristics and reduce the dimension. Finally, genetic algorithm (GA) is used to optimise the training of artificial neural network (ANN) to obtain the best improved GA‐ANN network model. It is used to analyse the voltage quality feature set, which further improves the accuracy of obtaining voltage quality anomalies and related reasons. Based on the established distribution station area, the proposed method is experimentally demonstrated. The results show that the accuracy of voltage quality anomaly monitoring and analysis exceeds 99.5% and 97% respectively, and the average accuracy of low‐voltage cause analysis reaches 97.83%, laying a theoretical foundation for building a strong distribution network.
Increased Galactosidase Beta 1 Expression as a Senescent Key Factor
in β-Cells Function Modulation at the Early Steps of Type 2
Diabetes
Authors: Maduro Ana T. , Pinto Anabela , Ferreira-Gomesb Joana , Costa Raquel , Soares Raquel , Luís Carla 
Published: April 2023

Abstract
Background In type 2 diabetes, insulin resistance is observed, and
β-cells are incapable of responding to glycemia demands, leading to
hyperglycemia. Although the nature of β-cells dysfunction in this
disease is not fully understood, a link between the induction of pancreatic
β-cell premature senescence and its metabolic implications has been
proposed. This study aimed to understand the relationship between diabetes and
pancreatic senescence, particularly at the beginning of the disease.
Methods C57Bl/6 J mice were fed two different diets, a
normal diet and a high-fat diet, for 16 weeks. Pancreatic histomorphology
analysis, insulin quantification, inflammation parameters, and senescence
biomarkers for the experimental animals were assessed at weeks 12 and 16.
Results The results proved that diabetes onset occurred at week 16 in the
High Fat Diet group, supported by glycaemia, weight and blood lipid levels.
Increased β-cells size and number accompanied by increased insulin
expression were observed. Also, an inflammatory status of the diabetic group was
noted by increased levels of systemic IL-1β and increased pancreatic
fibrosis. Finally, the expression of galactosidase-beta 1 (GLB1) was
significantly increased in pancreatic β-cells.
Conclusion The study findings indicate that senescence, as revealed by an
increase in GLB1 expression, is a key factor in the initial stage of
diabetes.
Cryofibrinogen—Characteristics and Association with Cryoglobulin: A Retrospective Study Out of a Series of 1,712 Samples over 7 Years
Authors: Sarda-Kolopp Marie N. , Miossec Pierre 
Published: April 2023

Objective Cryofibrinogens (CFs) and cryoglobulins (CGs) are cryoproteins responsible for obstructive vasculopathy and vasculitis. The aim of this study was to compare the characteristics of CF and CG, and to define the conditions of their association.
Methods and Results This retrospective study was conducted at the Lyon University Hospitals, and included patients with at least one sample tested for CF and/or CG between September 2013 and April 2021. Serum and plasma samples were analyzed in very strict conditions of temperature. After cold precipitation, CF and CG were characterized and quantified in the cryoprecipitates. CRP and plasma fibrinogen levels were also investigated. Over this 7-year period, 1,712 samples for CF detection and 25,650 samples for CG detection were sent to the laboratory. Simultaneous testing of CF and CG was performed in 1,453/1,712 samples (85%). CF was less often positive than CG (8.3 vs. 13.5%, p < 0.0001). In positive CF samples, CG was associated in 28.9% of cases. In CF, fibrinogen was associated with fibronectin in 98/142 (69%) samples, especially in highly concentrated CF. CF concentration was independent of C-reactive protein and plasma fibrinogen concentrations.
Conclusion The simultaneous detection of CF and CG is essential for the diagnosis of vasculitis or thromboembolic events and their treatment.
Taming SbH3: A Simple and Safe Approach to Using Stibane in Synthesis on a Laboratory Scale
Authors: von Hänisch Carsten , Dollberg Kevin , Schneider Selina 
Published: April 2023

AbstractHerein, we present a simple, inexpensive, and readily accessible apparatus for the safe and clean preparation, isolation, and conversion of stibane (SbH3). In this setup, only typical standard glassware and fabric tubes are used. The pure stibane can be obtained in very good yields with very little expenditure of time.
Urinary Bladder Mullerianosis Causing Recurrent Abdominal Pain: A Rare Case Report and Review of the Literature
Authors: Kumar Manjeet , Barwal Kailash Chander , Sharma Girish Kumar , Mardi Kavita , Raina Pamposh , Sharma Sanjeev 
Published: April 2023

AbstractMullerianosis of the urinary bladder is a rare bladder lesion with fewer than 30 cases reported in the literature. It describes the ectopic presence of endocervical, endometrium, and endosalpingial tissues inside the urinary bladder. It is diagnosed when at least two of three Mullerian tissues, endometriosis, endocervicosis, and endosalpingiosis, are identified. Mullerianosis presents in females of the reproductive age group, especially after pelvic surgery. Treatment involves resection of the bladder mass and ruling out malignant pathology. Follow-up of Mullerianosis is vital, as recurrence is common. Medical therapy is also indicated when a diagnosis is established after the histopathological examination of the tumor.We report a 31-year-old female presenting with recurrent lower abdominal pain. Cystoscopy showed a mass of 3 × 3 cm in the urinary bladder. Transurethral resection of bladder tumor was resected, and histopathology suggested a combination of endometriosis, endocervicosis, and endosalpingiosis. Pain was resolved after surgery, but the lesion recurred at 3 months, which was subsequently resected.
Risk Factors for Pneumothorax and Its Association with Ventilation in Neonates
Authors: Donda Keyur , Babu Sharmila , Rastogi Deepa , Rastogi Shantanu 
Published: April 2023

Objective The mode of ventilation that is implicated in pneumothorax is the one at the time of its diagnosis. Although there is evidence that air leak starts many hours before it is clinically evident, there are no prior studies that have investigated the association of pneumothorax with the mode of ventilation few hours before rather than at the time of its diagnosis.
Study Design A retrospective case–control study was conducted in the neonatal intensive care unit (NICU) between 2006 and 2016 where cases of neonates with pneumothorax were compared with gestational age-matched control neonates without pneumothorax. Respiratory support associated with pneumothorax was classified as the mode of ventilation 6 hours before the clinical diagnosis of pneumothorax. We investigated the factors that were different between cases and controls, and between cases of pneumothorax on bubble continuous positive airway pressure (bCPAP) and invasive mechanical ventilation (IMV).
Result Of the 8,029 neonates admitted in the NICU during the study period, 223 (2.8%) developed pneumothorax. Among these, 127 occurred among 2,980 (4.3%) neonates on bCPAP, 38 among 809 (4.7%) neonates on IMV, and the remaining 58 among 4,240 (1.3%) neonates on room air. Those with pneumothorax were more likely to be male, have higher body weight, require respiratory support and surfactant administration, and have bronchopulmonary dysplasia (BPD). Among those who developed pneumothorax, there were differences in the gestational age, gender, and use of antenatal steroids between those who were on bCPAP as compared to those on IMV. IMV was associated with increased odds of pneumothorax as compared to those on bCPAP in a multivariable regression analysis. Cases on IMV had higher incidence of intraventricular hemorrhage, retinopathy of prematurity, BPD, and necrotizing enterocolitis, as well as longer length of stay as compared to those on bCPAP.
Conclusion Neonates who require any respiratory support have higher incidence of pneumothorax. Among those on respiratory support, those on IMV had higher odds of pneumothorax and worse clinical outcomes as compared to those on bCPAP.
Key Points
Comparison of De-escalation of DAPT Intensity or Duration in East Asian and Western Patients with ACS Undergoing PCI: A Systematic Review and Meta-analysis
Authors: Gorog Diana A. , Jeyalan Visvesh , Markides Rafaella I. L. , Navarese Eliano P. , Jeong Young-Hoon , Farag Mohamed 
Published: April 2023

Background Guideline-recommended dual antiplatelet therapy (DAPT; aspirin plus prasugrel/ticagrelor) for 12 months in acute coronary syndrome (ACS) patients increases bleeding, with East Asians (EAs) exhibiting higher bleeding and lower ischemic risk, compared with non-East Asians (nEAs). We sought to compare DAPT “de-escalation” strategies in EA and nEA populations.
Methods A systematic review and meta-analysis of randomized controlled trials assessing reduction of DAPT intensity or duration in ACS patients undergoing percutaneous coronary intervention, in EA and nEA, was performed using a random-effects model.
Results Twenty-three trials assessed reduction of DAPT intensity (n = 12) or duration (n = 11). Overall, reduced DAPT intensity attenuated major bleeding (odds ratio [OR]: 0.78, 95% confidence interval [CI]: 0.65–0.94, p = 0.009), without impacting net adverse cardiovascular events (NACE) or major adverse cardiovascular events (MACE). In nEA, this increased MACE (OR: 1.20, 95% CI: 1.09–1.31, p < 0.0001) without impacting NACE or bleeding; while in EA, it reduced major bleeding (OR: 0.71, 95% CI: 0.53–0.95, p = 0.02) without affecting NACE or MACE. Overall, abbreviation of DAPT duration reduced NACE (OR: 0.90, 95% CI: 0.82–0.99, p = 0.03) due to major bleeding (OR: 0.69, 95% CI: 0.53–0.99, p = 0.006), without impacting MACE. In nEA, this strategy did not impact NACE, MACE, or major bleeding; while in EA, it reduced major bleeding (OR: 0.60, 95% CI: 0.4–0.91, p = 0.02) without impacting NACE or MACE.
Conclusion In EA, reduction of DAPT intensity or duration can minimize bleeding, without safety concerns. In nEA, reduction of DAPT intensity may incur an ischemic penalty, while DAPT abbreviation has no overall benefit.
Representations of ‘leftover women’ in the Chinese English-medium newspapers
Authors: Yu Yating , Lam Phoenix 
Published: April 2023

Abstract
Previous media studies have largely examined the media construction of ‘leftover women’ in the Chinese-medium news media that are pervaded by patriarchal content. Yet, little attention has been given to the thematic content surrounding leftover women in the Chinese English-medium news media which seem to be more liberal in their stance. To fill this niche, this study employs a key keyword analysis to examine the dominant thematic concepts related to leftover women in the Chinese English-medium news media. The findings illustrate that the key keywords in the news reports belong to three thematic categories, namely social actors and attributes, dating activities and relationships, and other social matters. These thematic categories are intertwined with one another to construct the overall representations of leftover women suggesting ideological implications for both patriarchy and egalitarianism. The usefulness of employing corpus techniques to examine overarching themes in gender representations is also highlighted in this study.