bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide association studies identify miRNA-194 as a prognostic biomarker for gastrointestinal cancer by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5
Authors: Huang Pan , Xia Lingyun , Guo Qiwei , Huang Congcong , Wang Zidi , Huang Yinxuan , Qin Shanshan , Leng Weidong , Li Dandan 
Published: December 2022

BackgroundThe dysregulated genes and miRNAs in tumor progression can be used as biomarkers for tumor diagnosis and prognosis. However, the biomarkers for predicting the clinical outcome of gastrointestinal cancer (GIC) are still scarce.MethodsGenome-wide association studies were performed to screen optimal prognostic miRNA biomarkers. RNA-seq, Ago-HITS-CLIP-seq, western blotting and qRT-PCR assays were conducted to identify target genes of miR-194. Genome-wide CRISPR-cas9 proliferation screening analysis were conducted to distinguish passenger gene and driver gene.ResultsA total of 9 prognostic miRNAs for GIC were identified by global microRNA expression analysis. Among them, miR-194 was the only one miRNA that significantly associated with overall survival, disease-specific survival and progress-free interval in both gastric, colorectal and liver cancers, indicating miR-194 was an optimal prognostic biomarker for GIC. RNA-seq analysis confirmed 18 conservative target genes of miR-194. Four of them, including ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5, were directly targeted by miR-194 and required for cell proliferation. Cell proliferation assay validated that miR-194 inhibits cell proliferation by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5 in GIC.ConclusionIn summary, miR-194 is an optimal biomarker for predicting the outcome of GIC. Our finding highlights that miR-194 exerts a tumor-suppressive role in digestive system cancers by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5.
Short-term outcomes of deeper intubation technique of ileus tube for different types of acute intestinal obstruction patients: A retrospective multicenter study
Authors: Tan Yanlu , Yin Fangxu , Lu Zhihua , Huang Peng , Zhang Chengcai , Sun Jiuzheng , Wang Song , Dong Zhensheng 
Published: December 2022

BackgroundOur previous research reported a novel deeper intubation technique (DIT) of the ileus tube for acute bowel obstruction patients. The present study was designed to evaluate the effect of this novel technique on the clinical outcomes of patients with obstruction using a large cohort.MethodsThe detailed clinical data were analyzed retrospectively from 496 obstruction patients who underwent intubation technique from 2014 to 2019 in five hospitals. The patients were divided into either the DIT group or the traditional intubation technique (TIT) group. The groups were matched in a 1:1 ratio using propensity scores, and the primary outcome was the short-term clinical outcomes for patients.ResultsThe baseline characteristics were similar between the DIT group and the TIT group after matching. Compared with the TIT group, the DIT group had a significantly deeper intubation depth, with shorter hospital days, shorter time to first flatus and defecation, lower pain score, increased drainage volume, and lower emergency surgery rate. Importantly, the inflammatory factors such as white blood cell, C-reactive protein, and procalcitonin levels were significantly lower in the DIT group. In addition, the DIT treatment was significantly useful for adhesive obstruction patients.ConclusionThe DIT procedure led to better short-term clinical outcomes compared with the TIT procedure, indicating that DIT is a safe and feasible technique for the treatment of intestinal obstruction that is worthy of further popularization and clinical application.
The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma
Authors: Qin Jiali , Yang Yang , Du Wei , Li Gang , Wu Yao , Luo Ruihua , Liu Shanting , Fan Jie 
Published: December 2022

BackgroundTo explore the metabolic differences of follicular thyroid carcinoma (FTC) by metabonomics, to find potential biomarkers for the diagnosis of FTC, and to explore the pathogenesis and diagnosis and treatment strategies of FTC.MethodThe metabonomics of 15 patients with FTC and 15 patients with follicular thyroid nodules(FTN) treated in Henan Cancer Hospital were analyzed by liquid chromatography-mass spectrometry (LC-MS).ResultsThe analysis showed that the metabolite profiles of FTC tissues could be well distinguished from those of control tissues, and 6 kinds of lipids were identified respectively, including lysophosphatidic acid(LysoPA) [LysoPA(0:0/18:0),LysoPA(0:0/18:2(9Z,12Z)],LysoPA[20:4(8Z,11Z,14Z,17Z)/0:0)]; phosphatidic acid(PA) [PA(20:3(8Z,11Z,14Z)/0:0),PA(20:4(5Z,8Z,11Z,14Z)/0:0),PA(20:5(5Z,8Z,11Z,14Z,17Z)/0:0)]; lysophosphatidylcholine(LPC) [LPC(18:1),LPC(16:0),LPC[16:1(9Z)/0:0],LPC(17:0),LPC[22:4(7Z,10Z,13Z,16Z),LPC(20:2(11Z,14Z); phosphatidylcholine(PC)(PC(14:0/0:0),PC(16:0/0:0); sphingomyelin(SM) (d18:0/12:0); fatty acid(FA)(18:1(OH3)]. There are 2 kinds of amino acids, including L-glutamate,L-glutamine.There are 3 other metabolites, including retinol,flavin adenine dinucleotide,androsterone glucuronide.Lipid metabolites are the main metabolites in these metabolites.The metabolic pathways related to FTC were analyzed by KEGG and HMDB, and 9 metabolic pathways were found, including 4 amino acid related metabolic pathways, 1 lipid metabolic pathways and 4 other related pathways.ConclusionThere are significant differences in many metabonomic characteristics between FTC and FTN, suggesting that these metabolites can be used as potential biomarkers. Further study found that LysoPA and its analogues can be used as biomarkers in the early diagnosis of FTC.It may be related to the abnormal metabolism of phospholipase D (PLD), the key enzyme of LysoPA synthesis caused by RAS pathway. At the same time, it was found that the metabolic pathway of amino acids and lipids was the main metabolic pathway of FTC. The abnormality of LysoPA may be the cause of follicular tumor carcinogenesis caused by lipid metabolic pathway.
Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)
Authors: Ren Hao , Zhang Hongling , Ni Ruoyao , Li Yanyan , Li Ling , Wang Wenhe , Tian Yu , Pang Baoping , Tan Yao 
Published: December 2022

In recent years, the leaf beetle Galeruca daurica has broken out in the northern grasslands of Inner Mongolia, its management still mainly depends on chemical control using traditional insecticides or with novel action. The study was aim to identify mutation locus associated with resistance to diamide insecticides in field population of G. daurica, to provide a reference for rational selection of insecticides and to avoid the rapid resistance development to diamide insecticides. We cloned the full length of the ryanodine receptor gene of G. daurica (GdRyR), constructed 3D model and transmembrane regions by homologous modeling based on deduced amino acid sequence. Two potential mutation loci (Gly4911Glu and Ile4754Met) and allelic mutation frequencies were detected in individuals of G. daurica. In addition, their binding patterns to two diamide insecticides (chlorantraniliprole, cyantraniliprole) were analyzed separately using a molecular docking method. The full-length cDNA sequence of GdRyR (GenBank accession number: OP828593) was obtained by splicing and assembling, which is 15,399 bp in length and encodes 5,133 amino acids. The amino acid similarity of GdRyR with that of other Coleopteran insects were 86.70%–91.33%, which possessed the typical structural characteristics. An individual resistance allelic mutation frequency test on fifty field leaf beetles has identified 12% and 32% heterozygous individuals at two potential mutation loci Gly4911Glu and Ile4754Met, respectively. The affinity of the I4754M mutant model of GdRyR for chlorantraniliprole and cyantraniliprole was not significantly different from that of the wild type, and all had non-covalent interactions such as hydrogen bonding, hydrophobic interactions and π-cation interactions. However, the G4911E mutant model showed reduced affinity and reduced mode of action with two diamide insecticides, thus affecting the binding stability of the ryanodine receptor to the diamide insecticides. In conclusion, the G4911E mutation in GdRyR may be a potential mechanism for the development of resistance to diamide insecticides on G. daurica and should be a key concern for resistance risk assessment and reasonable applications of diamide insecticides for control in future. Moreover, this study could provide a reference for ryanodine receptor structure-based insecticides design.
Adult Chinese Spanish L2ers’ acquisition of phi-agreement and temporal concord: The role of morphosyntactic features and adverb/subject-verb distance
Authors: Mao Tiaoyuan , Biondo Nicoletta , Zheng Zilong 
Published: December 2022

IntroductionWhile phi-agreement and concord are suggested to differ in nature during the first language (L1) acquisition, the acquisition of adverb-verb TC and SV person/number agreement by Chinese Spanish second language (L2) learners has only received limited attention. The current study examined morphosyntactic processing by advanced Chinese Spanish L2 learners (L2ers), whose L1 lacks the explicit morphological marking of tense and phi-agreement.MethodChinese Spanish L2ers and native Spanish speakers were asked to complete a self-paced grammaticality judgment task, where the grammaticality of adverb-verb TC and SV person/number agreement as well as the adverb/subject-verb distance were manipulated.ResultsFor both native Spanish speakers and L2ers, SV agreement violations are detected earlier and judged more accurately than adverb-verb TC violations. Furthermore, L2ers process SV number agreement less efficiently than SV person agreement (but as efficiently as adverb-verb TC). And there is no influence of the adverb/subject-verb distance on the processing of verbal inflection.ConclusionsThis study suggests that advanced Chinese Spanish L2ers tend to use native-like cognitive mechanisms for phi-agreement and concord computations, though their sensitivity to agreement violations may be further influenced by the morphosyntactic feature involved.
Reach and perceived effectiveness of a community-led active outreach postvention intervention for people bereaved by suicide
Authors: Hill Nicole T. M. , Walker Roz , Andriessen Karl , Bouras Hamza , Tan Shawn R. , Amaratia Punam , Woolard Alix , Strauss Penelope , Perry Yael , Lin Ashleigh 
Published: December 2022

BackgroundPostvention is a core component of suicide prevention strategies, internationally. However, the types of supports provided to people impacted by suicide vary widely. This study examines the perceived effectiveness of the Primary Care Navigator (PCN) model for people bereaved by suicide. The PCN model was implemented in response to a suicide cluster. It is an active outreach postvention intervention, initiated by police in response to a suspected suicide and links individuals to support in the immediate aftermath of their loss.MethodsA retrospective cross-sectional mixed methods approach was used to (1) identify the reach of the PCN model, (2) describe the type of support provided to people bereaved by a suspected suicide and (3) identify the perceived effectiveness of the PCN model from the perspective of WA police, postvention stakeholders and individuals bereaved by suicide. Quantitative data was used to examine the characteristics of suicide in the region, the characteristics of people who received bereavement support, and the types of support that were provided. Interviews with police, postvention stakeholders, and people bereaved by a suspected suicide were conducted to identify the perceived effectiveness of the intervention.ResultsBetween 1 January 2019 and 31 March 2021 there were 80 suspected suicides. Active outreach was provided to 347 bereaved individuals via the PCN model. Just under half of those who were offered outreach accepted further support (N = 164) in the form of suicide bereavement information (98%), mental health or clinical support (49.6%), specialized postvention counseling (38.4%), financial assistance (16%) and assistance with meals (16%), followed by housing assistance (14%) and referral to community services (11%). Police, stakeholders, and people with lived experience of a suspected suicide perceived the PCN model to be effective at connecting them to the community, linking people to support, and preventing suicide.ConclusionThe results provide evidence supporting the perceived effectiveness of an active outreach approach to postvention that provides acute support to people bereaved by suicide. Findings highlight important practical areas of support such as providing referral pathways and information on grief and suicide loss in the immediate aftermath of a suicide loss.
The changes in family functioning and family happiness during the COVID-19 pandemic: The situation in Thailand
Authors: Limsuwan Nida , Prachason Thanavadee , Wisajun Pattarabhorn 
Published: December 2022

BackgroundThe effects of the COVID-19 pandemic on family well-being and functioning were generally a concern for healthcare providers in many countries.ObjectivesTo explore the changes in family functioning and family happiness during the pandemic in Thailand and to investigate factors associated with the changes in family happiness.MethodsThis was a cross-sectional study conducted between November and December 2021. Online questionnaires regarding family functioning, family happiness, domestic violence, and COVID-19-related experiences were used.ResultsA total of 485 participants were included in this study. The perceived family happiness slightly decreased from 8.19 (pre-pandemic score) to 7.62 (post-pandemic score). In contrast, the general family functioning (SCORE-15 index), strength, and communication subscale scores after the onset of the COVID-19 pandemic were significantly lower than those of the pre-pandemic period. Moreover, the prevalence of verbal and physical violence significantly reduced during the pandemic. In addition, the change in family functioning was the strongest factor associated with the change in family happiness.ConclusionIn general, family functioning slightly improved; however, perceived family happiness decreased during the pandemic. In addition, the change in family functioning was the strongest factor associated with the change in family happiness.
Short-term effects of air pollutants on outpatients with psoriasis in a Chinese city with a subtropical monsoon climate
Authors: Wang Ting , Xia Yuanrui , Zhang Xinhong , Qiao Nini , Ke Susu , Fang Quan , Ye Dongqing , Fan Yinguang 
Published: December 2022

IntroductionPsoriasis is a common skin disease that seriously affects patients' quality of life. The association of air pollutants with psoriasis, and the extent of their effects remains unclear.MethodsBased on a distributed lag non-linear model, this study explored the short-term effects of air pollutants on outpatients with psoriasis in Hefei, China, between 2015 and 2019 by analyzing the exposure–lag–response relationship, after controlling for confounding influences such as meteorological factors, long-term trends, day of the week, and holidays. Stratified analyses were performed for patients of different ages and genders.ResultsThe maximum relative risks of psoriasis outpatients' exposure to SO2, NO2, and O3 were 1.023 (95% confidence intervals (CI): 1.004–1.043), 1.170 (95% CI: 1.046–1.307), and 1.059 (95% CI: 1.030–1.090), respectively. An increase of 10 μg/m3 of NO2 was associated with a 2.1% (95% CI: 0.7–3.5%) increase in outpatients with psoriasis, and a decrease of 10 μg/m3 of O3 was associated with an 0.8% (95% CI: 0.4–1.2%) increase in outpatients with psoriasis. Stratified analyses showed that male subjects were more sensitive to a change in meteorological factors, while female subjects and outpatients with psoriasis aged 0–17 years old were more sensitive to a change in air pollutants.DiscussionShort-term air pollutant exposures were associated with outpatients having psoriasis, suggesting that patients and high-risk people with psoriasis should reduce their time spent outside and improve their skin protection gear when air quality is poor.
Prevalence of anisometropia and associated factors in Shandong school-aged children
Authors: Xu Zihang , Wu Ziyun , Wen Ying , Ding Meihua , Sun Wei , Wang Yirong , Shao Zhen , Liu Yi , Yu Mingkun , Liu Guoyong , Hu Yuanyuan , Bi Hongsheng 
Published: December 2022

ObjectiveTo investigate anisometropia's prevalence and associated factors in school-aged children.MethodsA cross-sectional school-based study was conducted in Shandong Province, China, including children aged 4 to 17 from 9 schools. Anisometropia was defined as the differences between the two eyes in spherical equivalent (SE) or cylinder degree of 1.00 diopter (D) or more [SE or cylindrical (CYL) difference ≥ 1.00 D] after cycloplegic autorefraction. The Generalized Linear Model (GLM) was used to analyze the effects of ocular parameters [the differences between eyes in axial length (AL), habitual visual acuity (HVA), and corneal astigmatism (CA)] and lifestyle parameters (time spent indoor near work and outdoor activities) on anisometropia.ResultsTotal 4,198 (93.4%) of the 4,494 children were included in the statistical analysis. The mean difference in inter-eye SE was 0.42 ± 0.61 D. The prevalence of anisometropia was 13.2% (95%CI: 12.1 to 14.2%) (SE anisometropia's prevalence:10.3%; CYL anisometropia's prevalence: 4.1%), increased with older age (OR = 1.10, P = 0.002), the worse myopic eye (myopia vs. premyopia, OR = 1.87, P = 0.002), the worse hyperopic eye (hyperopia vs. premyopia, OR = 1.77, P = 0.013), larger difference in inter-eye AL (0.1–0.3 vs. ≤ 0.1, OR = 1.67, P = 0.008; &gt;0.3 vs. ≤ 0.1, OR = 28.61, P &lt; 0.001), HVA (&gt;0.2 vs. ≤ 0.2, OR = 3.01, P &lt; 0.001), CA (OR = 6.24, P &lt; 0.001), the worse stereoacuity (&gt;100 vs. ≤ 100, OR = 1.59, P = 0.001), longer indoor near work time per day on weekends (4–8 vs. &lt;4, OR = 1.41, P = 0.038; ≥8 vs. &lt;4, OR = 1.40, P = 0.131), and shorter outdoor activity time per day on weekdays (≥1 vs. &lt;1, OR = 0.75, P = 0.046) in multivariable analysis. In the SE anisometropia group, the difference in inter-eye AL (&gt;0.3 vs. ≤ 0.1, β: 0.556, 95%CI: 0.050 to 1.063), HVA (&gt;0.2 vs. ≤ 0.2, β: 0.511, 95%CI: 0.312 to 0.710), and CA (β: 0.488, 95%CI: 0.289 to 0.688), stereoacuity (&gt;100 vs. ≤ 100, β: 0.299, 95%CI: 0.110 to 0.488) had a positive impact on the difference in inter-eye SE.ConclusionsOcular parameters and lifestyle parameters are associated with the occurrence of anisometropia in children aged 4 to 17 years, including the difference in inter-eye AL, HVA, CA, stereoacuity, indoor near work time, and outdoor activity time. Preventing myopia and early treating anisometropic amblyopia may be effective ways to reduce the prevalence of anisometropia.
efeitos da gamificação no processo de ensino aprendizagem
Authors: Gonçalves Pereira Daniel , David José Maria N. 
Published: December 2022

O objetivo deste artigo é analisar como a utilização de recursos presentes na gamificação pode apoiar o aprendizado em um ambiente educacional utilizando a plataforma gamificada Matific voltada para disciplina de matemática. A pesquisa de abordagem qualitativa foi realizada com alunos do sexto ano do ensino fundamental em uma escola da rede pública no município de Governador Valadares – MG. Para tal, dividiu-se a turma em grupos de tratamento e controle que participaram de tarefas que nos permitiu concluir, a partir de um questionário e de observações que, um ambiente gamificado pode gerar resultados positivos no aprendizado do aluno como houve no grupo de tratamento desta pesquisa. A partir da pesquisa, foi possível compreender aspectos que podem afetar positivamente ou negativamente o andamento da aula e principalmente no aprendizado do aluno. Trabalhar com grupos menores contribuiu positivamente para a realização das atividades bem como a atenção dos alunos, um ambiente com muito barulho externo ou conversas intensas dispersa o foco da turma comprometendo a qualidade do aprendizado. Notou-se também que, o uso de recursos tecnológicos sem interação não gera resultados relevantes, o aluno precisa fazer parte da ação, é necessário que ele interaja com atividade a ponto que consiga perceber que há várias formas de atingir o mesmo objetivo, dessa forma ele estimula seu raciocínio logico e desenvolve novas habilidades.
Palavras-chave: Gamificação, Matific, Aluno, Engajamento, Tecnologia, Atividade gamificada.
HIRİSTİYAN SOSYALİZMİNİN ORTAYA ÇIKIŞI: ANGLİKAN SOSYALİZM DÜŞÜNCESİ
Authors: COSKUN Hacer 
Published: December 2022

Anglikan Sosyalizmi, Anglikan siyasi düşüncesi ile sosyalist düşünceyi sentezleyerek sanayileşme sonrası yaşanan toplumsal sorunlara çözüm üretmeye çalışmış ve böylece Hıristiyan sosyalizmi olarak kabul edilen düşüncenin oluşmasına öncü olmuştur. Dönemin dini ve siyasi koşulları çerçevesinde Anglikan siyasi düşüncesini klasik yorumlamanın ötesine taşıyarak devlet-kilise ilişkisine toplumsal olayları dahil etmişlerdir. Ayrıca yürütmüş oldukları kampanyalar ile bu düşünürler devletin politikalarına müdahale edebilen bir kilise politikası geliştirmeyi başarmışlardır. Dönemin ağır ekonomik koşulları karşısında çalışsan kesimi destekleyerek ve toplumsal reformların olması gerektiğini savunarak sosyalist söylem üretmişler ve çözüm için dinin rehberliğini önererek sosyalizme farklı bir soluk getirmişlerdir.
Bu çalışmada 19. ve 20. yüzyıllarda sanayileşme sonrasında toplumun sorunlarına çözüm üretmek amacıyla oluşmuş Anglikan Sosyalizm düşüncesinin oluşumu ve savundukları genel tezleri incelenmiştir.
Azerbaycan Muhasebe Hesap Planı ve Türkiye Tekdüzen Muhasebe Hesap Planı Karşılaştırılması
Authors: KARACAN Sami , RAHİMLİ Kenan 
Published: December 2022

Muhasebenin ana görevi, dış ve iç kullanıcılara bilgi sağlamaktır. Bilgileri doğru analiz ederek kullanan şirketler daha başarılı oluyor. Günümüz dünyasında teknolojik imkanların artması ve ülkeler arasındaki sınırların ortadan kalkması ile birlikte şirketler teknoloji, küreselleşme ve artan rekabet koşulları konularında kendilerini farklılaştırmaya devam etmektedirler. Farklı ülke ve kuruluşların farklı muhasebe bilgilerini herkesin anlayacağı bir standarda hazırlama zorunluluğunu getirir. Şu andan itibaren, birçok devlet IAS'ye dayalı olarak kendi standartlarını geliştiriyor.
Türkiye ve Azerbaycan ekonomik olarak birbirine yakındır. Küreselleşen dünyamız iki ülkeyi birbirine daha da yakınlaştırıyor. Bu çalışmanın amacı, Azerbaycan muhasebe sisteminde uygulanan hesap düzeni ile Türk muhasebe sisteminde uygulanan hesap düzeninin karşılaştırılması, incelenmesi, analiz edilmesi ve sonuçlarının değerlendirilmesidir.
Evaluación hidrogeológica para localizar fuentes de abastecimiento en Concepción Buenavista, Estado de Oaxaca, México
Authors: Vidal García Martín Carlos , Cárdenas Soto Martín , Escobedo Zenil David , García Serrano Alejandro 
Published: December 2022

El municipio de Concepción Buenavista se localiza en la Mixteca alta del estado de Oaxaca, México. Su población sufre escasez de agua debido al clima semiseco y a la limitada escorrentía superficial en la región. A fin de proponer alternativas de solución al problema de desabasto de agua, se realizó un estudio geológico-geofísico para determinar el sitio más adecuado para la perforación de un pozo que proporcione un caudal de 2 l/s, cantidad de agua necesaria para satisfacer las necesidades de la población. Los resultados permiten determinar un sitio que cumple las condiciones naturales favorables.
El sitio se caracteriza por la infiltración del agua superficial que proviene de un arroyo y de la recarga asociada al agua de lluvia.