bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein disulfide isomerase family is a potential therapeutic target in acute myeloid leukemia
Authors: Lin Li , Lu Yue , Zhang Yingkun , Qi Nannan , Huang Shuyi , Peng Meinan , Zhao Zhenzhen , Yang Aizhen , Niu Zhiyun , Wang Yan , Han Yue , Wu Yi , Zhang Jingyu 
Published: January 2025

Abstract

Acute myeloid leukemia (AML) as the second most common hematological malignancy remains currently incurable. In this study, we found the protein disulphide isomerase family (PDIs) was expressed in primary leukemia cells, with particular emphasis on ERP5 and ERP57. The compound PACMA31 demonstrated the ability to inhibit proliferation, enhance differentiation, exacerbate oxidative stress, and induce apoptosis in AML cells. RNA sequencing analysis revealed that PACMA31 impeded the proliferation of AML cells by modulating the unfolded protein response (UPR). Notably, Western blot results indicated that the antiproliferative effects of PACMA31 were mediated through the PERK/eIF2α signaling pathway. The selective inhibition of PDIs activity collectively induces apoptosis and differentiation in AML cells by activating the UPR via the PERK/eIF2α pathway. Consequently, PDIs inhibitors may represent promising candidates for the development of antitumor therapeutics against AML.
Enhancing Mesenchymal Stem Cell Functionality Through Nestin Overexpression: A Novel Approach for Treating End-Stage Liver Disease
Authors: He Xiaolong , Yang Ning , Sun Tao , Xue Junlong , Zhang Xue , Chu Jin , Li Liang , Liu Hui , Lü Guodong , Bi Xiaojuan , Lin Renyong 
Published: January 2025

Abstract

Transplantation of mesenchymal stem cells (MSCs) represents a novel therapeutic strategy for end-stage liver disease. However, the clinical application of MSCs is limited by challenges in their proliferation and procurement. In this study, we demonstrate that modified MSCs with Nestin overexpression can exhibit enhanced proliferation and migratory capabilities. A sufficient quantity of functional mitochondria is crucial for hepatocyte proliferation. Notably, Nestin overexpression improves the mitochondrial function of modified MSCs and facilitates hepatocyte proliferation through the transfer of mitochondria via tunneling nanotubes (TNTs). In Echinococcus multilocularis infection models, we observed that modified MSCs significantly enhanced hepatocyte proliferation and effectively reduced liver damage. Consequently, the transplantation of modified MSCs emerges as a potential therapeutic modality for the treatment of end-stage liver disease.
Oral microbiome in COPD patients: association with lung function and impacts of smoking and inhaled corticosteroids
Authors: Guo Rongrong , Wang Yongxin , Wu Shuo , Guo Ruirui , Zhang Junsong , Chen Xutao , Ti Xinyu 
Published: January 2025

Abstract

Background
The aim of our study was to describe the characteristics of oral microbiome in patients with chronic obstructive pulmonary disease (COPD) with different pulmonary function grades, and to investigate the impact of smoking and inhaled corticosteroids (ICS) on the oral microbiome in patients with various lung function impairments.
Methods
Pyrosequencing of polymerase chain reaction-amplified 16s rDNA genes was used to investigate the oral microbiome. COPD was diagnosed based on the Global Initiative for Chronic Obstructive Lung Disease (GOLD) Guidelines 2021.
Results
Bacterial richness and diversity increased in GOLD2-4 patients, and the beta-diversity analysis indicated differences between GOLD2-4 groups and CTRL-GOLD1 groups. Veillonella, Actinomyces, Escherichia-Shigella and Lactobacillus varies with different lung functions and there were different dominant strains in different groups, respectively. Functional genes of oral microbiome in GOLD2-4 patients also unfolded considerable discrepancy compared to CTRL. Smoking increased the abundance of Prevotella_7, Veillonella, Leptotrichia and Prevotella in GOLD 2–4 patients as well as the abundance of sixteen KEGG pathways. ICS treatment increased the diversity of the oral microbiome, and Prevotella and Porphyromonas were biomarkers found in the ICS group.
Conclusions
As lung function declined, the diversity, structure, distribution and signature of the oral microbiome changed, and the oral microbiomes of patients with different lung function had unique characteristics. Smoking and ICS affected the oral microbiome of COPD patients. Taken together, our findings may not only partially explain the mechanism of onset and development of COPD from perspective of the oral microbiome and its functional genes, but also provide an in-depth understanding of biomarker bacteria for COPD grades.
Risk factors for postoperative ileus following posterior spinal instrumentation in thoracic and lumbar spine fractures: a case control study
Authors: Fan Jixing , Cao Yuan , Cui Zengzhen , Zhu Tengjiao , Si Gao , Gao Shan , Lai Shengtao , Lv Yang , Zhou Fang 
Published: January 2025

Abstract

Background
The postoperative ileus (PI) was an unusual adverse effect, and the incidence and risk factors were still unclear. The purpose of present study was to clarify the prevalence of PI and elucidate the risk factors for PI following posterior spinal instrumentation in thoracic and lumbar spine fractures.
Methods
We retrospectively analyzed patients who developed PI after posterior spinal instrumentation in thoracic and lumbar spine fractures. For each PI patient, data were collected for two controls who underwent procedures in the same period and did not develop PI. Statistical methods included univariate and multivariate analyses. The logistic regression analysis was performed to evaluate risk factors of PI following posterior spinal instrumentation in thoracic and lumbar spine fractures.
Results
14 patients suffered from PI were included in the PI group and 28 patients without PI were included in the no PI group. Univariate analysis showed that previous abdominal surgery, a lower serum hemoglobin and albumin level, a longer LOS, neurologic deficit, a higher proportion of multi-level fractured vertebrae, longer operation time, greater estimated blood loss and RBC transfusion were associated with the occurrence of PI (P < 0.05). The multivariable logistic regression analysis showed that previous abdominal surgery (OR, 9.857; 95%CI, 1.069 ~ 90.860; p = 0.043) and multi-level fractured vertebrae (OR, 15.410; 95%CI, 2.136 ~ 111.158; p = 0.007) were the independent risk factors for PI (P < 0.05, respectively).
Conclusion
The PI should be considered as a differential diagnosis in patients with postoperative significant abdominal distension who had undergone posterior instrumentation for spinal trauma. A previous abdominal surgery and multi-level fractured vertebrae were independent risk factors for PI following posterior spinal instrumentation in thoracic and lumbar spine fractures. Knowledge of the associated predictive risk factors could potentially assist with the development of rigorous, evidence-based preventative strategies.
WITHDRAWN: Effect of pulse pests infestation sensitivity and ozone control on seed quality characteristics
Authors: Zinhoum Rasha A. , Omran Azza A. , Ebian Eman F. , Kassem Enas M.K. 
Published: January 2025

Abstract

The sensitivity of Egyptian pulses for bruchids beetle [Acanthoscelides obtectus (Say) and Callosobruchus maculatus] and mite (Acarus siro & Caloglyphus berlesei) infections and their impact on stored nutrients was investigated, as well as the effects of different ozone concentrations on controlling all stages of A. obtectus and mites infesting beans. The physicochemical quality properties of ozonated bean seeds were studied. For C. maculatus, the most significant weight loss was in cowpea, while A. obtectus showed the highest weight loss in common bean, while the faba bean, and chickpeas were the lowest. A. siro is more widespread than C. berlesi mites. The larval stage of A. obtectus was highly sensitive to ozone. A. siro was more tolerant to ozone than C. berlesei. Infected pulses increase moisture, while protein, crude fibers, fiber components, ash, fats, and phenolics decreased compared with non-infected, and the decrease rate was more significant in seeds infected with cowpea beetles than bean beetles. The lightness values, fats, and protein digestibility increased in ozonated beans, while the 100-seed weights, starch granule sizes, moisture, protein, and phenolic contents decreased compared with the control. Ozone enhanced the water absorption percentage and reduced the cooking time of beans, and they were acceptable for their sensory qualities compared with control samples.
The partial erythropoietin receptor agonist ML1-R is a potent neuroprotective drug with a distinct signaling profile.
Authors: Kwon Oh-Hoon , Bae Jinsik , Byun Jun Chul , Jeong Hyun-Joo , Liu Jixing , Lee Chang-Hun , Yoo Seung-Jun , Lee Seong-Ryong , Moon Cheil 
Published: January 2025

Abstract

Erythropoietin (EPO) is a glycoprotein that stimulates red blood cell production in the bone marrow and protects neurons from oxidative stress, making it a potential treatment for various neurological diseases. However, EPO analogs often lead to side effects like excessive erythropoiesis and tumor growth. In this study, we aimed to develop ML1-R, a peptide derived from the C-helix of EPO, to enhance neuroprotection while minimizing adverse effects. By modifying amino acids that interact with EPO receptors (EPORs), ML1-R activated EPORs differently from recombinant EPO (reEPO). ML1-R provided stronger neuroprotection than reEPO without promoting cell proliferation. In a murine stroke models and in-vitro neuron cultures, ML1-R reduced brain injury and prevented neuronal death caused by glutamate-induced excitotoxicity and hypoxia-reoxygenation. AlphaFold3 computational analysis showed distinct binding affinity and geometric structures between ML1-R–EPOR and EPO–EPOR complexes. ML1-R prolonged JAK2 activation and activated Akt/Erk signaling in distinct patterns, increasing EPORs on cell surface membranes. This reduced apoptosis and alleviated calcium overload, reactive oxygen species generation, and mitochondrial dysfunction induced by glutamate-induced excitotoxicity and hypoxia–reoxygenation. In conclusion, these findings highlight ML1-R as a promising candidate to treat ischemic stroke, reperfusion brain injury, and neurodegenerative diseases.
Utilizing C-Reactive Protein as a Predictor for Subclinical Mastitis
Authors: Sanjeevi Thiyagarajan , Sivaraman Valarmathi , Karunanithi Yogasri 
Published: January 2025

Abstract

Mastitis stands out as the most economically significant ailment in dairy cows due to its adverse effects on milk quality and reproductive performance, often leading to the involuntary culling of affected cows. Subclinical mastitis (SCM) can be considered a precursor to severe mastitis. This current study sheds light on the role of C-reactive protein (CRP) as a potential indicator for the presence of bovine SCM. The research involved the analysis of milk samples from a total of 49 cows, encompassing crossbred and native breeds. These samples were evaluated for their CRP levels and then compared with the absolute somatic cell count. To identify SCM, a somatic cell count within the range of 5.00-7.5 lakhs/ml was used as a threshold. The results revealed elevated CRP concentrations in the milk of cows afflicted with clinical mastitis (11.72 ± 0.75 µg/mL) as well as those with subclinical mastitis (10.9 ± 1.2 µg/mL), in stark contrast to the levels observed in healthy cows (0.33 ± 0.02 µg/mL). This research suggests that CRP may serve as a valuable tool for the ongoing surveillance of bovine mastitis, ultimately contributing to improved milk production performance.
Low Risk Covid-19 Travel Map – A Generic Solution
Authors: SAURAV SWAPNIL , Peter J Hencil , Dwivedi Jaiprakash Narain 
Published: January 2025

Abstract

Whole world has seen the havoc created by Coronavirus disease (COVID-19), which is an infectious disease. 'CO' stands for corona, 'VI' for virus, and 'D' for disease. Formerly, this disease was referred to as '2019 novel coronavirus' or '2019-nCoV.' The COVID-19 virus is a new virus linked to the same family of viruses as Severe Acute Respiratory Syndrome (SARS) and some types of common cold. The recovery rate for someone suffering from COVID-19 varies from 92%-97% around the world, which is probably not a cause of concern. More concerning is that older people, and those with underlying medical problems like cardiovascular disease, diabetes, chronic respiratory disease, and cancer are more likely to develop serious illness and even can lead to death. The major concerns for the governments around the world was to control the transmission of this disease. The world which was known to be interconnected suddenly started closing borders. Even the states within countries sealed their borders. Flights were disrupted. Hospitality industry suffered a great financial loss. According to The World Travel and Tourism Council (WTTC), tourism generated $240 bn or 9.2% of India's GDP in 2018 and supported 42.67 mn jobs which is 8.1% of its total employment. The industry saw major job loss and contraction in its revenues. This was the biggest motivator to carry out this research. Challenge is to come up with best possible routes during Covid-19 times and this could be extended to any such pandemic we might encounter in future as well.
We propose two standard approaches to calculate risk for the travel locations in this paper. The two approaches are:

Approach 1: Risk calculation based on main travel locations’
active cases, and
Approach 2: Risk calculation based on main travel locations and
locations’ immediate neighbours

In our research, Covid-19 dataset for India has been used and that has been downloaded from the government official website. Though the proposed solution can be applied in any country/area map, our experiment is mainly focus India as we have used India’s Covid-19 dataset. Our proposed algorithms connects with Google Maps and suggest top 3 low risk routes for the traveller. We want to make sure the world remains connected and no other such pandemic should stop the movement of goods or people.
Emergent dynamics of active elastic microbeams
Authors: Palacci Jérémie , Martinet Quentin , Li Yuting , Aubret Antoine , Hannezo Edouard 
Published: January 2025

Abstract

In equilibrium, the physical properties of matter are set by the interactions between the constituents. In contrast, the energy input of the individual components controls the behavior of synthetic or living active matter. Great progress has been made in understanding the emergent phenomena in active fluids, though their inability to resist shear forces hinders their practical use. This motivates the exploration of active solids as shape-shifting materials, yet, we lack controlled synthetic systems to devise active solids with unconventional properties. Here we build active elastic beams from dozens of active colloids and unveil complex emergent behaviors such as self-oscillations or persistent rotations. Developing tensile tests at the microscale, we show that the active beams are ultra-soft materials, with large (non-equilibrium) fluctuations. Combining experiments, theory, and stochastic inference, we show that the dynamics of the active beams can be mapped on different phase transitions which are tuned by boundary conditions. More quantitatively, we assess all relevant parameters by independent measurements or first-principles calculations, and find that our theoretical description agrees with the experimental observations. Our results demonstrate that the simple addition of activity to an elastic beam unveils novel physics and can inspire design strategies for active solids and functional microscopic machines.
Hematite (U-Th)/He thermochronometry unveils unique exhumation history: An example from the Dexing porphyry copper deposit, Southern China
Authors: Zhang Hong , Wang Yu , An Fang , Yuan Honglin , Gao Jianfeng 
Published: January 2025

Abstract
Hematite, commonly found in endogenetic deposits, plays a crucial role in monitoring cooling history temperature variations and can be used to conduct (U-Th)/He dating. Hematite thermochronometry also provides the clearest guide to the timing of the latest thermal evolution path combined with apatite and zircon (U-Th)/He dating. In this study, we established a new method of hematite (U-Th)/He thermochronometry based on an optimized analytical protocol to reveal the evolution of the latest uplift and cooling histroy of the Dexing porphyry copper deposit (DPCD), South China. Two hematite samples were collected from the Tongchang and Fujiawu mines in the DPCD yielded age distributions at 92-11.2 Ma and 112-24 Ma, and gave closure temperatures between 180 and 215 °C based on hematite particle sizes of 88-199 μm. Combined with the published (U-Th)/He thermochronological data on zircon and apatite, this study proposes that the DPCD may have experienced a prolonged temperature derease thermal history. The initial exhumation and uplift were happened at 112 Ma, then a rapid uplift occurred from 11.2 to 8.0 Ma. This study also demonstrates that the hematite (U-Th)/He dating can be effectively applied to reveal the uplift and preservation history of porphyry copper deposits.
Doxazosin Attenuates Development of Testosterone Propionate-induced Prostate Growth by regulating TGF-β/Smad Signaling Pathway, Prostate-specific Antigen Expression and Reversing Epithelial-Mesenchymal Transition in Mice and Stroma Cells
Authors: Li YiDan , Tu BingHua , Wang ZiTong , Shao ZiChen , Fu ChenHao , Hua JianQiang , Zhang ZiWen , Zhang Peng , Sun Hui , Mao ChenYan , Liu Chi-Ming 
Published: January 2025

Objectives:
The present study examined the effects of doxazosin on testosterone propionate (TP)-induced prostate growth in vivo and in vitro and its impact on
the EMT and TGF-β/Smad signaling pathway.

Methods:
Mice were treated with TP. Doxazosin (5 or 10 mg/kg) and finasteride (10 mg/kg) were administered orally for 28 days in TP-induced mice. The
prostate index (prostate/body weight ratio), morphological characteristics and the protein expression of the prostate were examined. We further
examined the effects of doxazosin and finasteride on the EMT and TGF-β/Smad signaling pathway in mice and in human prostate stroma cell
(WPMY-1). The protein expressions of TGF-β1, TGFBR2, p-Smad2/3, N-cadherin, vimentin, fibronectin and α-SMA, E-cadherin and prostate
specific antigen (PSA) were determined after treatment by western blot.

Results:
The prostate wet weight, prostate index decreased after treatment. Doxazosin (5 or 10 mg/kg), finasteride (10 mg/kg) or a combination treatment
(doxazosin 10 mg/kg + finasteride 10 mg/kg) were shown to reverse the pathological and morphological characteristics of the prostate. Doxazosin
and finasteride inhibited TP-induced prostate growth, EMT, and the TGF-β/Smad signaling pathway by downregulating the expression of TGF-β1,
TGFBR2, p-Smad2/3, N-cadherin, vimentin, fibronectin and α-SMA, whereas expression of E-cadherin was increased after treatment with either
doxazosin or finasteride. Doxazosin (1-50 μM) inhibited normal human prostate stroma cell growth (WPMY-1) after 48 h with or without
testosterone treatment. Doxazosin also regulated the EMT and proteins related to the TGF-β/Smad signaling pathway in WPMY-1 cells after 24 h.
Additionally, doxazosin decreased protein expression of the PSA both in vivo and in vitro.

Conclusion:
This study demonstrated that doxazosin inhibits prostate growth by regulating the EMT and TGF-β/Smad signaling pathways in the prostate. This
finding suggests that doxazosin has potential as a new signaling pathway for the treatment of BPH.
The Effect of Audit Burden on Subsequent Tax Evasion
Authors: Hageman Amy M. , LaMothe Ethan G. , Marshall Mary E. 
Published: January 2025

ABSTRACT
Audited taxpayers can be compelled to expend both monetary and nonmonetary resources during an audit, irrespective of compliance levels. We refer to these audit-related expenditures as “audit burden,” and examine how they impact subsequent compliance decisions. Motivated by cost-loss framing theory, we predict audit burden influences individuals who were initially noncompliant (compliant) on an audited return to subsequently increase (decrease) compliance. Consistent with hypotheses, results from our first experiment suggest burdensome audits deter noncompliance for those who evaded, but also precipitates noncompliance for initially compliance taxpayers. A second experiment shows results are robust to alternative operationalizations of audit burden. Mediation analysis from our third experiment suggests the effect of audit burden results from differences in how groups respond to the perceived expenditure of audit burden. Finally, results from our fourth and final experiment suggest a simple apology can weaken the compliance-reducing effect of burdensome audits on initially compliant individuals.
Data Availability: Data are available from the authors upon request.
JEL Classifications: H26.
Work ability following breast cancer – the MyHealth randomized controlled trial
Authors: Horsbøl Trine A. , Saltbæk Lena , Urhammer Caroline , Karlsen Randi V. , Johansen Christoffer , Bidstrup Pernille E. , Høeg Beverley L. , Zoffmann Vibeke , Belmonte Federica , Andersen Ingelise , Friberg Anne S. , Svendsen Mads N. , Christensen Helle G. , Glavicic Vesna , Nielsen Dorte L. , Dalton Susanne O. 
Published: January 2025

Background and purpose: We previously demonstrated positive effects on quality of life and mental health following breast cancer when comparing a nurse-led follow-up program without scheduled visits (MyHealth) to regular follow-up. This study aims to examine whether MyHealth also positively impacts self-reported work ability.
Patients/material and methods: A total of 288 patients, potentially active on the labour market, were randomized to MyHealth or control follow-up after primary treatment for early-stage breast cancer (2017–2019). MyHealth included individual self-management sessions, electronic symptom monitoring, and assistance with navigating healthcare services. Control follow-up consisted of biannual outpatient visits with a physician. Linear mixed-effect models were applied to evaluate the effect of MyHealth on self-reported work ability at 6, 12, 24, and 36 months after randomization as measured by the Work Ability Score (WAS).
Results: Work ability increased significantly in both groups during the first 6 months (mean WAS increase MyHealth: 1.64, 95% confidence interval [CI]: 1.26; 2.02 and control: 1.57, 95% CI: 1.17; 1.97) and continued to increase slightly but non-significantly (p-values > 0.13) until end of follow-up at 36 months. Improvement was especially pronounced among patients reporting poor work ability at baseline. Differences in mean WAS between patients in MyHealth and control follow-up were non-significant and close to zero at all time points (–0.21 to 0.48).
Interpretation: The MyHealth follow-up program had no additional effect on self-reported work ability compared to regular follow-up. Future interventions should target patients with poor work ability and include components specifically designed to enhance work ability.