bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATATÜRK DÖNEMİ TBMM ZABIT CERİDELERİNE GÖRE TÜRK KADINININ BİREY OLMA SÜRECİ (1920-1938)
Authors: DOĞUALP ÇİÇEK Hazine 
Published: May 2023

Atatürk Dönemi Türk kadın haklarının gelişim sürecinin ele alındığı bu çalışmada zaman, mekân ve dinî inanca göre kadınlara olan yaklaşım incelenmeye çalışılmıştır. İslamiyet öncesi Türk tarihinde erkekle denk tutulan kadın, İslamiyetle birlikte değişen kültürün de etkisiyle geri planda kalmıştır. Zamanla hakları kesintiye uğrayan Türk kadınları, Cumhuriyetin ilanı ve Atatürk’ün dünya görüşü çerçevesinde yeniden şekillenmiş ve hak ettiği yeri bulmuştur. Eğitimden istihdama kadar birçok yönde erkekle eş tutulan Türk kadını eğitim ve kültürde de yükselmiş, çağdaş bir kimliğe bürünmüştür.
Atatürk Dönemi kadın hakları meselesi üzerinde durulan ve bilimsel alanda çok çalışılan bir konu olmuştur. Ancak bu çalışmalar kaynak bakımından birbirini tekrar etmiş ya da içerik olarak yüzeysel, bilinegelmiş konulardan oluşmuştur. Kanunların oluşum sürecini gözler önüne seren ve meclisteki görüşmelerin kayıt altına alındığı TBMM Zabıt Cerideleri ise çalışmalarda belli başlı konular dışında kullanılmamıştır. Bu nedenle çalışmanın ana kaynağını TBMM Zabıt Cerideleri oluşturmuştur. Atatürk Dönemi meclis zabıtlarının ele alındığı çalışmada kadınların sosyal, siyasi, eğitim, ekonomik ve medeni hallerini ilgilendiren tüm kayıtlar kullanılmaya özen gösterilmiştir.
The Meaning of Register in Yoga from a Sociolinguistic Perspective
Authors: Lailiyah Nur , Waryanti Endang 
Published: May 2023

This study describes the form and meaning of registers in yoga in Kediri and explores the influence on yoga practitioners (yogis). The data sources for this research consisted of informants, including Acarya (instructors) and Yogis, while the data was in the form of lexicons uttered by Yogis and Acarya. Data were collected by observing techniques with advanced techniques; listening, engaging, and talking. The data analysis technique uses the theory of register forms resulting from a combination adapted to the register forms in yoga. This study found that there were 53 registers in yoga exercises consisting of two forms, namely, single and complex. There are 29 singular forms and 24 complex forms. The yoga register also strongly influences Yogis (doers), including spirituality, attitudes, and relationships with others. Also, it overcomes health problems faced daily which focus on meditation activities. Meanwhile, the meaning of register in the choice of speech style is intended by Acharya to inspire enthusiasm and freshness in communicating; besides that, it also marks a communication function that has the uniqueness of being a registered marker that has a specific meaning based on context.
Sulfasalazine induces autophagy inhibiting neointimal hyperplasia following carotid artery injuries in mice
Authors: Zhang Weichang , Yan Cheng , Xiao Yangyan , Sun Yuxin , Lin Youjun , Li Qinglong , Cai Wenwu 
Published: May 2023

Background: Neointimal hyperplasia (NH) is a crucial pathophysiological feature in vascular transplant and in-stent restenosis. Excessive proliferation and migration of vascular smooth muscle cells (VSMCs) play important roles in neointimal hyperplasia. This study aims to explore the potentialities and mechanism of sulfasalazine (SSZ) in the prevention of restenosis.Methods: Sulfasalazine was encapsulated in nanoparticles made of poly (lactic-co-glycolic acid) (PLGA). In vivo, carotid ligation injury was induced in mice to induce Neointimal hyperplasia, with or without sulfasalazine containing nanoparticles (NP-SSZ) treatment. After 4 weeks, the arteries were collected for histology, immunofluorescence, Western blotting (WB) and qRT-PCR. In vitro, vascular smooth muscle cells were treated with TNF-α to induce cell proliferation and migration, followed by SSZ or vehicle treatment. WB was performed to further explore its mechanism.Results: The ratio of intima to media thickness (I/M) was increased after ligation injury on day 28, while the ratio was significantly reduced in the NP-SSZ treatment group. The dual positive nuclei of Ki-67 and α-SMA were 47.83% ± 9.15%, whereas only 29.83% ± 5.98% in the NP-SSZ-treated group (p &lt; 0.05). Both MMP-2 and MMP-9 were decreased in the NP-SSZ treatment group (p &lt; 0.05, p &lt; 0.05, respectively) compared to the control group. The levels of the targeted inflammatory genes (TNF-α, VCAM-1, ICAM-1, MCP-1) were lower in the NP-SSZ treatment group compared with the control group. In vitro, the proliferating cell nuclear antigen (PCNA) expression was significantly decreased in the SSZ treatment group. The cell viability of VSMCs was markedly increased in the TNF-α treatment group, whereas sulfasalazine treatment inhibited this effect. LC3 II and P62 protein expression were higher in the SSZ group than in the vehicle group both in vitro and in vivo. The phosphorylation of NF-kB (p-NF-kB) and the phosphorylation of mTOR (p-mTOR) were decreased in the TNF-α+ SSZ group, whereas the P62 and LC3 II expression levels were increased. However, the expression level of p-mTOR, P62, and LC3 II was reversed after co-treatment with the agonist of mTOR MHY1485, whereas the p-NF-kB expression level was unchanged.Conclusion: sulfasalazine inhibited vascular smooth muscle cells proliferation and migration in vitro and Neointimal hyperplasia in vivo through NF-kB/mTOR-mediated autophagy.
Single-cell atlas of dental pulp stem cells exposed to the oral bacteria Porphyromonas gingivalis and Enterococcus faecalis
Authors: Zhang Wen , Xu Tiansong , Li Xueying , Zhang Yifei , Zou Xiaoying , Chen Feng , Yue Lin 
Published: May 2023

Introduction:Porphyromonas gingivalis and Enterococcus faecalis promote the development of pulpitis and periapical periodontitis. These bacteria are difficult to eliminate from the root canal systems, leading to persistent infection and poor treatment outcomes. We explored the response of human dental pulp stem cells (hDPSCs) to bacterial invasion and the mechanisms underlying the impact of residual bacteria on dental pulp regeneration.Methods: Single-cell sequencing was used to categorize the hDPSCs into clusters based on their response to P. gingivalis and E. faecalis. We depicted a single-cell transcriptome atlas of hDPSCs stimulated by P. gingivalis or E. faecalis.Results: The most differentially expressed genes in the Pg samples were THBS1, COL1A2, CRIM1, and STC1, which are related to matrix formation and mineralization, and HILPDA and PLIN2, which are related to the cellular response to hypoxia. A cell cluster characterized by high expression levels of THBS1 and PTGS2 was increased after P. gingivalis stimulation. Further signaling pathway analysis showed that hDPSCs prevented P. gingivalis infection by regulating the TGF-β/SMAD, NF-κB, and MAPK/ERK signaling pathways. Differentiation potency and pseudotime trajectory analyses showed that hDPSCs infected by P. gingivalis undergo multidirectional differentiation, particularly to the mineralization-related cell lineage. Furthermore, P. gingivalis can create a hypoxia environment to effect cell differentiation. The Ef samples were characterized by the expression of CCL2, which is related to leukocyte chemotaxis, and ACTA2, which is related to actin. There was an increased proportion of a cell cluster that was similar to myofibroblasts and exhibited significant ACTA2 expression. The presence of E. faecalis promoted the differentiation of hDPSCs into fibroblast-like cells, which highlights the role of fibroblast-like cells and myofibroblasts in tissue repair.Discussion: hDPSCs do not maintain their stem cell status in the presence of P. gingivalis and E. faecalis. They differentiate into mineralization-related cells in the presence of P. gingivalis and into fibroblast-like cells in the presence of E. faecalis. We identified the mechanism underlying the infection of hDPSCs by P. gingivalis and E. faecalis. Our results will improve understanding of the pathogenesis of pulpitis and periapical periodontitis. Furthermore, the presence of residual bacteria can have adverse effects on the outcomes of regenerative endodontic treatment.
Epigenetic and transcriptomic alterations in the ClC-3-deficient mice consuming a normal diet
Authors: Jing Zhenghui , Zhang Haifeng , Wen Yunjie , Cui Shiyu , Ren Yuhua , Liu Rong , Duan Sirui , Zhao Wenbao , Fan Lihong 
Published: May 2023

Introduction: Metabolic disorders are an important health concern that threatens life and burdens society severely. ClC-3 is a member of the chloride voltage-gated channel family, and ClC-3 deletion improved the phenotypes of dysglycemic metabolism and the impairment of insulin sensitivity. However, the effects of a healthy diet on transcriptome and epigenetics in ClC-3−/− mice were not explained in detail.Methods: Here, we performed transcriptome sequencing and Reduced Representation Bisulfite Sequencing for the liver of 3 weeks old WT and ClC-3−/− mice consuming a normal diet to insight into the epigenetic and transcriptomic alterations of ClC-3 deficient mice.Results: In the present study, we found that ClC-3−/− mice that were younger than 8 weeks old had smaller bodies compared to ClC-3+/+ mice with ad libitum self-feeding normal diet, and ClC-3−/− mice that were older than 10 weeks old had a similar body weight. Except for the spleen, lung, and kidney, the average weight of the heart, liver, and brain in ClC-3−/− mice was lower than that in ClC-3+/+ mice. TG, TC, HDL, and LDL in fasting ClC-3−/− mice were not significantly different from those in ClC-3+/+ mice. Fasting blood glucose in ClC-3−/− mice was lower than that in ClC-3+/+ mice; the glucose tolerance test indicated the response to blood glucose increasing for ClC-3−/− mice was torpid, but the efficiency of lowering blood glucose was much higher once started. Transcriptomic sequencing and reduced representation bisulfite sequencing for the liver of unweaned mice indicated that ClC-3 deletion significantly changed transcriptional expression and DNA methylation levels of glucose metabolism-related genes. A total of 92 genes were intersected between DEGs and DMRs-targeted genes, of which Nos3, Pik3r1, Socs1, and Acly were gathered in type II diabetes mellitus, insulin resistance, and metabolic pathways. Moreover, Pik3r1 and Acly expressions were obviously correlated with DNA methylation levels, not Nos3 and Socs1. However, the transcriptional levels of these four genes were not different between ClC-3−/− and ClC-3+/+ mice at the age of 12 weeks.Discussion: ClC-3 influenced the methylated modification to regulate glucose metabolism, of which the gene expressions could be driven to change again by a personalized diet-style intervention.
Variation in CD8 T cell IFNγ differentiation to strains of Toxoplasma gondii is characterized by small effect QTLs with contribution from ROP16
Authors: Kongsomboonvech Angel K. , García-López Laura , Njume Ferdinand , Rodriguez Felipe , Souza Scott P. , Rosenberg Alex , Jensen Kirk D. C. 
Published: May 2023

IntroductionToxoplasma gondii induces a strong CD8 T cell response characterized by the secretion of IFNγ that promotes host survival during infection. The initiation of CD8 T cell IFNγ responses in vitro differs widely between clonal lineage strains of T. gondii, in which type I strains are low inducers, while types II and III strains are high inducers. We hypothesized this phenotype is due to a polymorphic “Regulator Of CD8 T cell Response” (ROCTR).MethodsTherefore, we screened F1 progeny from genetic crosses between the clonal lineage strains to identify ROCTR. Naïve antigen-specific CD8 T cells (T57) isolated from transnuclear mice, which are specific for the endogenous and vacuolar TGD057 antigen, were measured for their ability to become activated, transcribe Ifng and produce IFNγ in response to T. gondii infected macrophages.ResultsGenetic mapping returned four non-interacting quantitative trait loci (QTL) with small effect on T. gondii chromosomes (chr) VIIb-VIII, X and XII. These loci encompass multiple gene candidates highlighted by ROP16 (chrVIIb-VIII), GRA35 (chrX), TgNSM (chrX), and a pair of uncharacterized NTPases (chrXII), whose locus we report to be significantly truncated in the type I RH background. Although none of the chromosome X and XII candidates bore evidence for regulating CD8 T cell IFNγ responses, type I variants of ROP16 lowered Ifng transcription early after T cell activation. During our search for ROCTR, we also noted the parasitophorous vacuole membrane (PVM) targeting factor for dense granules (GRAs), GRA43, repressed the response suggesting PVM-associated GRAs are important for CD8 T cell activation. Furthermore, RIPK3 expression in macrophages was an absolute requirement for CD8 T cell IFNγ differentiation implicating the necroptosis pathway in T cell immunity to T. gondii.DiscussionCollectively, our data suggest that while CD8 T cell IFNγ production to T. gondii strains vary dramatically, it is not controlled by a single polymorphism with strong effect. However, early in the differentiation process, polymorphisms in ROP16 can regulate commitment of responding CD8 T cells to IFNγ production which may have bearing on immunity to T. gondii.
Efficacy and Safety of intravenous monoclonal antibodies in patients with moderate-to-severe active Graves’ophthalmopathy: a systematic review and meta-analysis
Authors: Hu Yu , Chen Jinhua , Lin Ken , Yu Xijie 
Published: May 2023

BackgroundsThe effects of various treatments on Graves’ ophthalmopathy (GO) have been studied. As monoclonal antibodies (mAbs) have been proposed for the treatment of moderate to severe GO, direct comparisons between different mAbs are lacking.We therefore conducted this meta-analysis to objectively compare the efficacy and safety of intravenous mAbs.MethodsTo identify eligible trials, references published before September 2022 were electronically searched in PubMed, Web of Science, Pubmed, Embase,Cochrane Library, CBM, CNKI,Wan-Fang and ICTRP databases.The Newcastle-Ottawa scale (NOS) and the Cochrane Risk of Bias Assessment Tool were used to assess the risk of bias of the original studies.The primary and secondary outcomes were the response and inactivation rates, with the secondary outcomes being the clinical activity score (CAS),the improvement of proptosis and diplopia improvement,and the adverse event rate. Publication bias was evaluated, along with subgroup and sensitivity analyses.ResultsA total of 12 trials with 448 patients were included. The meta-analysis showed that TCZ (tocilizumab) was most likely to be the best treatment in terms of response according to indirect contrast, followed by TMB (teprotumumab) and RTX (rituximab).TCZ, followed by TMB and RTX, was also most likely to be the best treatment in terms of reducing proptosis. In terms of improving diplopia, TMB was most likely to be the best treatment, followed by TCZ and RTX.TCZ was the highest probability of safety, followed by RTX and TMB.ConclusionsBased on the best available evidence,TCZ should be the preferred treatment for moderate to severe GO.In the absence of head-to-head trials,indirect comparisons of treatments are routinely used to estimate the effectiveness of the treatments of interest. In addition,the optimal dose and potential mechanism of action of monoclonal antibodies remain to be established,and it is encouraging that the treatment paradigm for GO may change in the future.This study was designed in accordance with the Preferred Reporting Items for conducting Systematic Reviews and Meta-Analyses (PRISMA)(27).Systematic Review Registrationhttp://www.crd.york.ac.uk/prospero, identifier CRD42023398170.
Hydrogen peroxide formation in carbon clothes for enhancement of an electro-oxidation tertiary treatment for tequila vinasse wastewater
Authors: Covarrubias-Del-Toro Rafael , Huerta-Rocha Marisol , Lezama Luis , García Edith Xio Mara , Estrada-Vargas Arturo 
Published: May 2023

Carbon-based electrodes have been extensively used as cathodes for the electro-generation of H₂O₂, which is used in the production of the hydroxyl radical •OH to mineralize organic compounds in several types of wastewater. Carbon cloth has been also used in combination with activated carbon for the combined adsorption and oxidation of persistent organic compounds present in tequila vinasse wastewater. Whereas most of the works regarding the H₂O₂ electro-generation involve relatively complex processes to enhance the catalytic activity or the electrochemically active area of these electrodes, calcination by itself represents a simple and low-cost option to enhance these cathodic and anodic functions, especially in the fabrication of large area electrodes that could be needed to treat the large amount of tequila vinasse wastewater that is produced daily. In this work, the effect of calcination at 300°C and 600°C of carbon clothes in the oxidation current and H₂O₂ production at different potentials in H₂SO₄ was studied. Oxidation current increased 700% upon calcination at 600°C, compared to no calcination. H₂O₂ was produced only in a narrow range of polarizations, whereas calcination at 600 °C increased the generation rate from 7.1 ± 0.3 to 17.8 ± 0.4 mg L⁻1 h⁻1.
Autoimmune/inflammatory syndrome induced by adjuvants in a woman with Hashimoto thyroiditis and familial autoimmunity—a case report and literature review
Authors: Plavsic Aleksandra , Arandjelovic Snezana , Dimitrijevic Milan , Kusic Natasa , Tomic Spiric Vesna , Popovic Bojana , Jovicic Zikica , Peric Popadic Aleksandra , Miskovic Rada 
Published: May 2023

IntroductionAutoimmune/inflammatory syndrome induced by adjuvants (ASIA) consists of a wide spectrum of symptoms and immunological features that are believed to develop in predisposed individuals after exposure to an adjuvant, including a silicone breast implant (SBI). Different autoimmune diseases (AIDs) have been associated with ASIA, but ASIA development after SBI in women with Hashimoto thyroiditis (HT) and familial autoimmunity has rarely been described.Case reportA 37-year-old woman presented in 2019 with arthralgia, sicca symptoms, fatigue, + antinuclear antibody (ANA), + anti SSA, and + anticardiolipin Immunoglobulin G (IgG) antibodies. She was diagnosed with HT and vitamin D deficiency in 2012. The familial autoimmunity was present: the patient’s mother had been diagnosed with systemic lupus erythematosus and secondary Sjogren’s syndrome and her grandmother with cutaneous lupus and pernicious anemia. In 2017, the patient had a cosmetic SBI procedure that was complicated by repeated right breast capsulitis. After 2 years of irregular visits due to COVID-19, she presented with + ANA, + anticentromere antibodies both in sera and seroma, sicca syndrome, arthralgias, twinkling in extremities, abnormal capillaroscopic findings, and reduced diffusing capacity of the lungs for carbon monoxide. She was diagnosed with ASIA, and antimalarial and corticosteroid therapy were introduced.ConclusionIn patients with HT and familial autoimmunity, SBI should be carefully considered due to the possibility of ASIA development. Hashimoto thyroiditis, familial autoimmunity, and ASIA seem to be interconnected in the complex mosaic of autoimmunity in predisposed individuals.
Serum proteomics in giant cell arteritis in response to a three-day pulse of glucocorticoid followed by tocilizumab monotherapy (the GUSTO trial)
Authors: Christ Lisa , Gloor Andrea D. , Kollert Florian , Gaber Timo , Buttgereit Frank , Reichenbach Stephan , Villiger Peter M. 
Published: May 2023

ObjectiveProteome analyses in patients with newly diagnosed, untreated giant cell arteritis (GCA) have not been reported previously, nor are changes of protein expression upon treatment with glucocorticoids (GC) and/or tocilizumab (TCZ) known. The GUSTO trial allows to address these questions, provides the opportunity to learn about the differential effects of GC and TCZ on proteomics and may help to identify serum proteins to monitor disease activity.MethodsSerum samples obtained from 16 patients with new-onset GCA at different time points (day 0, 3, 10, and week 4, 24, 52) during the GUSTO trial (NCT03745586) were examined for 1436 differentially expressed proteins (DEPs) based on proximity extension assay technology. The patients received 500 mg methylprednisolone intravenously for 3 consecutive days followed by TCZ monotherapy.ResultsWhen comparing day 0 (before the first GC infusion) with week 52 (lasting remission), 434 DEPs (213↑, 221↓) were identified. In response to treatment, the majority of changes occurred within 10 days. GC inversely regulated 25 proteins compared to remission. No difference was observed between weeks 24 and 52 during established remission and ongoing TCZ treatment. Expression of CCL7, MMP12, and CXCL9 was not regulated by IL6.ConclusionDisease-regulated serum proteins improved within 10 days and were normalized within 24 weeks, showing a kinetic corresponding to the gradual achievement of clinical remission. The proteins inversely regulated by GC and TCZ shed light on the differential effects of the two drugs. CCL7, CXCL9, and MMP12 are biomarkers that reflect disease activity despite normalized C-reactive protein levels.
Targeting NETs using dual-active DNase1 variants
Authors: Englert Hanna , Göbel Josephine , Khong Danika , Omidi Maryam , Wolska Nina , Konrath Sandra , Frye Maike , Mailer Reiner K. , Beerens Manu , Gerwers Julian C. , Preston Roger J. S. , Odeberg Jacob , Butler Lynn M. , Maas Coen , Stavrou Evi X. , Fuchs Tobias A. , Renné Thomas 
Published: May 2023

BackgroundNeutrophil Extracellular Traps (NETs) are key mediators of immunothrombotic mechanisms and defective clearance of NETs from the circulation underlies an array of thrombotic, inflammatory, infectious, and autoimmune diseases. Efficient NET degradation depends on the combined activity of two distinct DNases, DNase1 and DNase1-like 3 (DNase1L3) that preferentially digest double-stranded DNA (dsDNA) and chromatin, respectively.MethodsHere, we engineered a dual-active DNase with combined DNase1 and DNase1L3 activities and characterized the enzyme for its NET degrading potential in vitro. Furthermore, we produced a mouse model with transgenic expression of the dual-active DNase and analyzed body fluids of these animals for DNase1 and DNase 1L3 activities. We systematically substituted 20 amino acid stretches in DNase1 that were not conserved among DNase1 and DNase1L3 with homologous DNase1L3 sequences.ResultsWe found that the ability of DNase1L3 to degrade chromatin is embedded into three discrete areas of the enzyme's core body, not the C-terminal domain as suggested by the state-of-the-art. Further, combined transfer of the aforementioned areas of DNase1L3 to DNase1 generated a dual-active DNase1 enzyme with additional chromatin degrading activity. The dual-active DNase1 mutant was superior to native DNase1 and DNase1L3 in degrading dsDNA and chromatin, respectively. Transgenic expression of the dual-active DNase1 mutant in hepatocytes of mice lacking endogenous DNases revealed that the engineered enzyme was stable in the circulation, released into serum and filtered to the bile but not into the urine.ConclusionTherefore, the dual-active DNase1 mutant is a promising tool for neutralization of DNA and NETs with potential therapeutic applications for interference with thromboinflammatory disease states.