bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing TCAB1 suppresses proliferation of hepatocellular carcinoma cells by inducing apoptosis
Authors: Zhang Guangmou , Zhang Kefeng , Yuan Meng , Yuan Zhiqing 
Published: April 2023

Abstract

Objectives
Telomerase Cajal body protein 1 (TCAB1) is closely related to the occurrence, development and prognosis of tumors, and may affect the sensitivity of tumor radiotherapy. There are no reports about the effect of TCAB1 gene expression on proliferation and apoptosis of HEPG2 cells. We plan to investigate whether silencing TCAB1 using siRNA is helpful for the diagnosis and treatment of hepatocellular carcinoma.

Methods
Three siRNA sequences (siTCAB1-1, siTCAB1-2, siTCAB1-3) targeting TCAB1 gene and one negative sequence (NC) were designed, synthesized and then transfected into HEPG2 cells, separately. The expressions of TCAB1 and telomerase reverse transcriptase (TERT) in mRNA and protein level were detected by RT-qPCR and Western blot assays. Moreover, proliferation and apoptosis of HEPG2 cells were measured by MTT assay, and flow cytometry, respectively.

Results
RT-qPCR and Western blot data both showed that TCAB1 expression in the siTCAB1 group was significantly lower than that in the blank control and NC groups (p<0.05). However, TERT expression was not significantly different among those groups (p>0.05). MTT result showed that HEPG2 cells proliferation in the siTCAB1 group was lower than that in the blank control and NC groups (p<0.05). The apoptotic rate in the siTCAB1 group was significantly increased compared with the blank control and NC groups (p<0.01).

Conclusions
Silencing TCAB1 can inhibit proliferation and promote apoptosis of HEPG2 cells, providing a potential therapeutic method for diagnosis and treatment of hepatocellular carcinoma.
Synaptojanin1 Modifies Endolysosomal Parameters in Cultured Ventral Midbrain Neurons
Authors: Zhu Xinyu , Prakash Sanjana Surya , McAuliffe Geoffrey , Pan Ping-Yue 
Published: April 2023

AbstractThe accumulation of α-synuclein (α-syn)-enriched protein aggregates is thought to arise from dysfunction in degradation systems within the brain. Recently, missense mutations ofSYNJ1encoding the SAC1 and 5’-phosphatase domains have been found in families with hereditary early-onset Parkinsonism. Previous studies showed thatSynj1haploinsufficiency (Synj1+/−) leads to accumulation of the autophagy substrate p62 and pathologic α-syn proteins in the midbrain (MB) and striatum of aged mice. In this study, we aim to investigate the neuronal degradation pathway using theSynj1+/− MB culture from mouse pups of mixed sex as a model. Our data show that GFP-LC3 puncta formation and cumulative mKeima puncta formation are unaltered at baseline inSynj1+/− MB neurons. However, GFP-LAMP1 puncta is reduced with a similar decrease in endogenous proteins, including lysosomal-associated membrane protein (LAMP)1, LAMP2, and LAMP2A. The LAMP1 vesicles are hyperacidified with enhanced enzymatic activity inSynj1+/− MB neurons. Using a combination of light and electron microscopy (EM), we show that endolysosomal changes are primarily associated with a lack of SAC1 activity. Consistently, expressing the SYNJ1 R258Q mutant in N2a cells reduces the lysosome number. Interestingly, the endolysosomal defects inSynj1+/− neurons does not impact the clearance of exogenously expressed wild-type (WT) α-syn; however, the clearance of α-syn A53T was impaired in the axons ofSynj1+/− MB neurons. Taken together, our results suggest axonal vulnerability to endolysosomal defects in Synj1-deficient MB neurons.
Analisis Validitas dan Reliabilitas Sosial Budaya dan Organisasi terhadap Adopsi E-Commerce UMKM Tangerang Selatan
Authors: Huda Muhammad Qomarul , Kusumaningtyas Rinda Hesti , Aini Qurrotul , Hidayah Nur Aeni , Yulian Icha 
Published: April 2023

Tingginya perkembangan teknologi informasi, sejalan dengan tingginya terobosan yang dapat dilakukan bagi masyarakat. E-commerce menjadi salah satu contoh teknologi yang sudah berkembang dan banyak membantu masyarakat hingga saat ini. Pelaku UMKM banyak yang sudah beralih menggunakan e-commerce sebagai media penjualan namun dalam penerapannya masih memiliki hambatan. Penelitian ini bertujuan untuk menginvestigasi hambatan sosial budaya dan organisasi bagi pelaku UMKM Tangerang Selatan dengan menggunakan framework hambatan adopsi e commerce Zaied. Berdasarkan hasil uji validitas untuk keseluruhan item yang diajukan menunjukkan hasil yang valid. Kemudian hasil uji reliabilitas untuk mengetahui konsistensi jawaban dari responden mendapatkan nilai 0,830 dengan kata lain memiliki nilai yang reliabel. Penelitian ini memberikan hasil bahwa adanya hambatan pada nilai, keyakinan, pelaku UMKM dalam menerapkan e-commerce dengan angka sebesar 58% pada sosial & budaya. Hambatan lainnya pada organisasi mengenai kurangnya dukungan manajemen dan memilki keterbatasan akses informasi dengan angka sebesar 63% dalam menerapkan e-commerce sebagai media penjualan.
Optimization of Personal Protective Equipment Distribution Costs to Health Centers with Stepping Stone Method
Authors: Nugroho Arief Budi , Utami Meinarini Catur , Putro Suryo Santoso 
Published: April 2023

This study aims to apply the Stepping Stone transportation method to optimize transportation costs for sending personal protective equipment (PPE) to thirteen health centers in Pamekasan. The method used is the Stepping Stone transportation method because it can manage the distribution of several warehouses that provide products to be sent to places in need optimally, which was previously a problem due to the length of time for sending medicines and PPE to each health centre and shipping operational costs are increasing because the distance of each warehouse to each health centre in each sub-district is very influential for cost as well as time. Based on the results of transportation simulations with this method, the results of the distribution cost of PPE delivery to each health centre in Pamekasan were obtained with distribution to Pamekasan warehouses of 30,000 units, Pakong warehouses of 15,000 units, Tlanakan warehouses of 10,000 units, and Pasean warehouses of 19,500 units, with optimal costs of IDR 37,000,000. The contribution of this research can be used by the Pamekasan District Health Office as an alternative policy for distributing PPE to each health centre in Pamekasan at an optimal cost.
The Political Identity: Between Qur'anic View and Western Theory
Authors: Zubaidi Sujiat , Asnawi Aqdi Rofiq , Sansayto Ide Mafaza , Putri Humaira Agam 
Published: April 2023

Purpose:Identity nowadays has become very complicated in the political sphere. Political identity needs to be studied from two perspectives; Western theory and Islamic theory. Islamic political identity taken from the concept of the Qur'an. This research aims to reveal identity politics from these two perspectives, namely the perspective of the al-Qur'an and Western theory. Method:The library research method is used for this critical analysis research, which takes a qualitative approach. The qualitative method collected data and news references from books, journals, or trusted online sources. Results/Findings:The results show that there are contradictions in interpreting identity politics. Identity politics is often interpreted as something that is considered harmful. Through critical analysis, researchers found differences from the most fundamental aspects of understanding and theory to the value of their influence on society. Conclusion:Even though the theory of identity in the West existed and was developed by Henry Tajfel and John Turner, the theory is in line with how Islam views political identity as necessary as a political strategy that emphasizes differentiation and the utilization of primordial ties as its main category. Identity in the Qur'an is called Shibgah. The theory of identity, both in politics and outside politics, is correct and reasonable.
Optimal and Efficient Auctions for the Gradual Procurement of Strategic Service Provider Agents
Authors: Farhadi Farzaneh , Chli Maria , Jennings Nicholas R. 
Published: April 2023

We consider an outsourcing problem where a software agent procures multiple services from providers with uncertain reliabilities to complete a computational task before a strict deadline. The service consumer’s goal is to design an outsourcing strategy (defining which services to procure and when) so as to maximize a specific objective function. This objective function can be different based on the consumer’s nature; a socially-focused consumer often aims to maximize social welfare, while a self-interested consumer often aims to maximize its own utility. However, in both cases, the objective function depends on the providers’ execution costs, which are privately held by the self-interested providers and hence may be misreported to influence the consumer’s decisions. For such settings, we develop a unified approach to design truthful procurement auctions that can be used by both socially-focused and, separately, self-interested consumers. This approach benefits from our proposed weighted threshold payment scheme which pays the provably minimum amount to make an auction with a monotone outsourcing strategy incentive compatible. This payment scheme can handle contingent outsourcing plans, where additional procurement happens gradually over time and only if the success probability of the already hired providers drops below a time-dependent threshold. Using a weighted threshold payment scheme, we design two procurement auctions that maximize, as well as two low-complexity heuristic-based auctions that approximately maximize, the consumer’s expected utility and expected social welfare, respectively. We demonstrate the effectiveness and strength of our proposed auctions through both game-theoretical and empirical analysis.
Complicaciones cutáneas de la terapia inmunosupresora: estudio en trasplantados renales
Authors: Orozco Beatriz , Jaramillo Diego E. 
Published: April 2023

Se presenta la experiencia dermatológica obtenida durante el seguimiento prospectivo de 114 pacientes con trasplante renal, atendidos en el Hospital Universitario San Vicente de Paúl, de Medellín; su evolución post-trasplante fluctuaba entre 2 meses y 13 años y el seguimiento dermatológico fue por dos años. Se encontró un número elevado de manifestaciones cutáneas (promedio 4,8 por paciente); las más frecuentes fueron: acné, micosis, hirsutismo y xerosis; no se hallaron lesiones malignas. No hubo asociación de las manifestaciones cutáneas con la edad del paciente, el tipo de donante o el esquema Inmunosupresor. Dada la alta frecuencia de patología cutánea se sugiere que estos pacientes sean educados adecuadamente sobre este aspecto y examinados periódicamente con el fin de detectar y manejar a tiempo sus problemas dermatológicos benignos o potencialmente malignos.
Piomiositis en el Hospital San Juan de Dios, Armenia, Quindío
Authors: Ramírez Luis A. , Ruiz Emilsen 
Published: April 2023

Se presenta una serie de 12 pacientes con piomiositis, diagnosticados entre 1983 y 1988 en el Hospital Universitario San Juan de Dios, de Armenia, Quindío. Se trata de la casuística más numerosa informada hasta ahora en Colombia sobre esta enfermedad e incluye cuatro casos de etiología diferente al Staphylococcus aureus, a saber: tres por Staphylococcus epidermidis y uno por Klebsiella oxytoca. Clínicamente y por laboratorio no hubo diferencias significativas según la etiología. El antecedente de trauma fue Infrecuente (25%), la utilidad diagnóstica de la determinación de las enzimas séricas dé origen muscular muy baja y el ultrasonido muy útil en la localización del absceso muscular.
Evaluación de la clonidina y la testosterona en el manejo del retraso constitucional del crecimiento: informe preliminar sobre 21 pacientes
Authors: Uribe Londoño Federico , Balthazar Vital , Orrego Arturo , Saumet Fabio , Restrepo Ana E. 
Published: April 2023

Los sujetos de este estudio fueron 15 niños y 6 niñas con retraso constitucional del crecimiento (edad 13.4 ±3.1 años, rango 10.9-16). Trece niños y una niña se encontraban en la etapa prepuberal al Iniciar el estudio. Con el fin de observar la velocidad de crecimiento (VC) espontánea, los pacientes fueron seguidos por un periodo de cuatro meses antes de Iniciar el tratamiento. Los niños menores de 13 años y todas las niñas recibieron clonidina durante 4 a 12 meses (0.1 mg/m2/día). Con ésta, el grupo de niños Incrementó su velocidad de crecimiento de 5.9 ± 3.12 a 7.04 ± 2.23 cm/año y las niñas de 5.53 ± 2.31 a 6.45 ± 1.18 cm/año (Ns). Sin embargo, hubo dos grupos claramente discernibles: los que respondieron a la clonidina y los que no lo hicieron. En los niños que respondieron la VC espontánea antes de la clonidina fue significativamente menor que la de los que no respondieron (3.8 ± 3.27 vs. 7.67 ±1.66 cm/año) ( p < 0.05). Para las niñas las cifras fueron 3.5 ± 0.86 y 7.57 ± 0.4 cm/año, respectivamente (p < 0.01 ). Nueve niños recibieron enantato de testosterona (250 mg IM/mes por cuatro dosis); cinco de ellos hablan recibido clonidina previamente. La VC antes de la testosterona (5.0 :I: 2.59 cm/año) en comparación con post- testosterona (9.26 ±2.4 cm/año) ,fue significativamente menor (p < 0.01 ). El retraso de la maduración esquelética definido por el índice edad ósea/edad cronológica no influyó en la respuesta eventual a la clonidina, ni tal respuesta fue atribuible a un incremento en el índice de maduración sexual (Tanner) durante el tratamiento con esta droga. Se concluye que una velocidad de crecimiento espontánea subnormal (menos de 4 cm/año) parece tener un valor predictivo para una eventual respuesta de estos niños a la clonidina. Se necesita un mayor número de casos para conclusiones más definitivas.
Fernando Zambrano Ulloa 1940-1991
Authors: Díaz González Federico 
Published: April 2023

IATREIA lamenta profundamente la muerte del Doctor Fernando Zambrano U/loa, Profesor Honorario de la Universidad de Antioquia. El Profesor Zambrano fue uno de los fundadores de esta revista y, hasta el último momento, formó parte de su Comité Editorial; en éste su labor se caracterizó como en toda su vida universitaria, por la disciplina, la honestidad y el compromiso
Psoriasis y dermatomicosis
Authors: Valencia Carlos , Vélez Herta , Santamaría de Uribe Lucía , Montoya Fernando , Vargas Maria E. 
Published: April 2023

Se realizó estudio micológico a 52 pacientes que tenían diagnóstico clínico e histopatológico de psoriasis, pertenecientes al servicio de dermatología del Hospital Universitario San Vicente de Paúl, de Medellín, entre agosto de 1991 y febrero de 1993. Se tomaron 109 muestras a partir de las placas psoriáticas y de las lesiones sospechosas de dermatomicosis. En 10 casos (19.2%) se corroboró el diagnóstico de dermatomicosis; en 5 de ellos se encontró onicomicosis por Candida albicans sin asociación con la edad, el sexo, el oficio O el tratamiento de los pacientes. En 4 hombres se aisló E. floccosum de diferentes localizaciones y en una mujer 7: tonsurans de lesiones interdigitales en los pies. Se demostró una asociación estadísticamente significativa entre la Infección por dermatofitos y el uso de esteroides sistémlcos (p = 0.021 ). No se obtuvo crecimiento de dermatofitos a partir de las placas psoriáticas ni se vieron cambios histológicos típicos de la enfermedad en las lesiones producidas por los hongos. En conclusión, es baja la frecuencia de dermatomicosis en personas con psoriasis; usualmente la piel comprometida por los hongos está libre de cambios psoriáticos y el riesgo de contraer la dermatofitomicosis se incrementa en unas 30 veces en los pacientes tratados con esteroides sistémicos.
Knowledge, attitudes, and practices regarding legal and illegal substances
Authors: Montalvo Prieto Amparo , Castillo Avila Irma Yolanda 
Published: April 2023

Objective. This article sought to describe knowledge, attitudes, and practices regarding the use of legal and illegal substances by nursing students from Cartagena (Colombia).
Methodology. This was a descriptive study conducted on a probabilistic sample of 689 students matriculated in three nursing programs. The study used the Predisposing Factors instrument associated to the use of psychoactive substances by Cepeda, Aldana, and Ossío.
Results. The mean age of the participants was 20.5 years, 91.9% were women, 92.4% belonged to socio-economic levels 1 to 3, 87.5% were single. Of the 12 psychoactive substances (PAS) consulted, eight were considered by over 90% of the students as harmful to health. A total of 94% considered that the brain is the organ most affected by drug consumption. The students considered production (80.4%), trafficking (79.4%), and use of substances (80.0%) as problems of great importance; and they agreed with investing financial resources for prevention, rehabilitation, and follow-up programs for the population affected (89.1%). They expressed that use of PAS is mainly influenced by friends (26.9%) and by family problems (26.7%). The highest life prevalence of legal PAS use were: alcohol (77.6%) and cigarettes (17.6%); along with marihuana for illegal PAS use (1.8%).
Conclusion. Knowledge of nursing students on legal and illegal PAS is not satisfactory, although they have favorable attitudes for their prevention and control. Use of PAS by the students, although not of great magnitude, is a problem deserving attention from organisms in charge of university welfare programs. Curricular contents should be enhanced on the phenomenon of PAS use of future nursing professionals.
Teaching humanization in undergraduate nursing course subjects
Authors: Vieira de Almeida Débora , Corrêa Chaves Eliane 
Published: April 2023

Objective. To describe teaching on humanization in the subjects included in nursing curricula in São Paulo city (Brazil).
Method. Descriptive and cross-sectional study of subjects included in the curricula of all Higher Education Institutions (HEI) accredited by the Ministry of Education (Brazil) in São Paulo City. Humanization in nursing was defined as the encounter of subjectivities in and through the care act.
Results. Thirteen curricula were studied, totaling 588 subjects. Although 59% contained some term related to humanization, when assessing compatibility with the definition used in the study, findings showed: 3% full compatibility, 28% partial, 62% no compatibility and, in 7% of the subjects, comparison was impossible.
Conclusion. The undergraduate nursing subjects analyzed showed ambiguous intentionality as, although the majority contained some term related to humanization, only a minority was in accordance with the concept.
Nursing actions in the perioperative period and in preparing prostatectomy patients for discharge
Authors: Ferreira da Mata Luciana Regina , Caldas Ferreira Taciana , Campos de Carvalho Emília 
Published: April 2023

Objective. To identify nursing actions in the perioperative period and in preparing prostatectomy patients for discharge.
Methodology. Cross-sectional, retrospective study. Data were collected from medical record of patients who underwent partial or total prostatectomy between August 2009 and August 2010 at hospitals in Divinopolis, Minas Gerais, Brazil.
Results. A total of 121 patients were identified; the mean age was 67 years. The most frequent diagnosis was prostate cancer (70%). Main preoperative activities were measuring vital signs (55%), administering drugs (52%), educating patients about fasting (50%), and managing edema (45%). After surgery, the most frequent tasks were measuring vital signs (100%), measuring urine and emptying the urinary bag (100% for each), facilitating the healing of surgical wounds (77%), and evaluating Penrose drain discharge (48%). Among the 25 types of care observed, the activity that nurses performed most often for patients about to be discharged was providing education about urinary catheter manipulation (16%).
Conclusion. This study identified weaknesses in care delivery for prostatectomy patients, particularly regarding home care education.
Being in front of the patient. Nurse-patient interaction and use of technology in emergency services
Authors: Granados-Pembertty Yeimy Yesenia , Arias- Valencia María Mercedes 
Published: April 2023

Objective. This study sought to describe how the use of technology intervenes in the nurse-patient relationship, from the nurse's point of view.
Methodology. This was a qualitative research with tools from grounded theory. Twenty semi-structured interviews were conducted with nurses working in emergency services in three municipalities of Colombia.
Results. Four categories emerged: 1) direct care, the maximum interaction or being in front of the patient; 2) fairly direct care; 3) indirect care, institutional management; and 4) minimum interaction; technology as facilitator of the interaction and awareness of the necessity for interaction.
Conclusion. This study shows the irreplaceable nature of the nurse and the fundamental necessity of technology. The dual mediations of technology constitute a paradoxical matter that reveals the importance of placing it as a means; warning on the danger of converting it an end in and of itself.
Using the internet to exchange information and experience on cystic fibrosis
Authors: Cargnin Pimentel Ellen Dayane , Dos Santos Luz Geisa , Marisa Pelloso Sandra , Barros Carvalho Maria Dalva 
Published: April 2023

Objective. Describing the experience of patients with cystic fibrosis (CF) and their families using the internet as a means for information, interaction and exchange of experiences with this disease.
Methodology. A descriptive qualitative exploratory study was carried out in 2011. The subjects of the study were patients with CF and their parents or other family members contacted via e-mail on social networking websites. The requested information included the guiding question: ''Tell me about your experience on the internet as a means of information and exchange of experiences involving CF'', as well as demographic information and whether the respondent was the patient or a family member.
Results. A total of 40 invitations were sent by email asking for information and 22 were accepted (55%). According to participants (15 patients and seven family members), the most widely used internet media were websites related to seeking information about CF. The use of internet was divided in three categories: 1) scientific and technical aspects, 2) emotional aspects, and c) modes of disseminating information.
Conclusion. The internet proved to be a medium of opinion formation which could eventually replace medical advice. It is necessary to establish criteria for constructing and monitoring information related to CF published on internet websites.