bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibitor-3 inhibits Protein Phosphatase 1 via a metal binding dynamic protein–protein interaction
Authors: Srivastava Gautam , Choy Meng S. , Bolik-Coulon Nicolas , Page Rebecca , Peti Wolfgang 
Published: March 2023

AbstractTo achieve substrate specificity, protein phosphate 1 (PP1) forms holoenzymes with hundreds of regulatory and inhibitory proteins. Inhibitor-3 (I3) is an ancient inhibitor of PP1 with putative roles in PP1 maturation and the regulation of PP1 activity. Here, we show that I3 residues 27–68 are necessary and sufficient for PP1 binding and inhibition. In addition to a canonical RVxF motif, which is shared by nearly all PP1 regulators and inhibitors, and a non-canonical SILK motif, I3 also binds PP1 via multiple basic residues that bind directly in the PP1 acidic substrate binding groove, an interaction that provides a blueprint for how substrates bind this groove for dephosphorylation. Unexpectedly, this interaction positions a CCC (cys-cys-cys) motif to bind directly across the PP1 active site. Using biophysical and inhibition assays, we show that the I3 CCC motif binds and inhibits PP1 in an unexpected dynamic, fuzzy manner, via transient engagement of the PP1 active site metals. Together, these data not only provide fundamental insights into the mechanisms by which IDP protein regulators of PP1 achieve inhibition, but also shows that fuzzy interactions between IDPs and their folded binding partners, in addition to enhancing binding affinity, can also directly regulate enzyme activity.
All-fibre phase filters with 1-GHz resolution for high-speed passive optical logic processing
Authors: Kaushal Saket , Aadhi A. , Roberge Anthony , Morandotti Roberto , Kashyap Raman , Azaña José 
Published: March 2023

AbstractPhotonic-based implementation of advanced computing tasks is a potential alternative to mitigate the bandwidth limitations of electronics. Despite the inherent advantage of a large bandwidth, photonic systems are generally bulky and power-hungry. In this respect, all-pass spectral phase filters enable simultaneous ultrahigh speed operation and minimal power consumption for a wide range of signal processing functionalities. Yet, phase filters offering GHz to sub-GHz frequency resolution in practical, integrated platforms have remained elusive. We report a fibre Bragg grating-based phase filter with a record frequency resolution of 1 GHz, at least 10× improvement compared to a conventional optical waveshaper. The all-fibre phase filter is employed to experimentally realize high-speed fully passive NOT and XNOR logic operations. We demonstrate inversion of a 45-Gbps 127-bit random sequence with an energy consumption of ~34 fJ/bit, and XNOR logic at a bit rate of 10.25 Gbps consuming ~425 fJ/bit. The scalable implementation of phase filters provides a promising path towards widespread deployment of compact, low-energy-consuming signal processors.
Identification and characterization of novel ETV4 splice variants in prostate cancer
Authors: Cosi Irene , Moccia Annalisa , Pescucci Chiara , Munagala Uday , Di Giorgio Salvatore , Sineo Irene , Conticello Silvestro G. , Notaro Rosario , De Angioletti Maria 
Published: March 2023

AbstractETV4, one of ETS proteins overexpressed in prostate cancer, promotes migration, invasion, and proliferation in prostate cells. This study identifies a series of previously unknown ETV4 alternatively spliced transcripts in human prostate cell lines. Their expression has been validated using several unbiased techniques, including Nanopore sequencing. Most of these transcripts originate from an in-frame exon skipping and, thus, are expected to be translated into ETV4 protein isoforms. Functional analysis of the most abundant among these isoforms shows that they still bear an activity, namely a reduced ability to promote proliferation and a residual ability to regulate the transcription of ETV4 target genes. Alternatively spliced genes are common in cancer cells: an analysis of the TCGA dataset confirms the abundance of these novel ETV4 transcripts in prostate tumors, in contrast to peritumoral tissues. Since none of their translated isoforms have acquired a higher oncogenic potential, such abundance is likely to reflect the tumor deranged splicing machinery. However, it is also possible that their interaction with the canonical variants may contribute to the biology and the clinics of prostate cancer. Further investigations are needed to elucidate the biological role of these ETV4 transcripts and of their putative isoforms.
Determinants of people’s motivations to approach COVID-19 vaccination centers
Authors: Keser Claudia , Rau Holger A. 
Published: March 2023

AbstractThis paper presents the results of a survey exploring the determinants of vacinees’ confidence in COVID-19 vaccines and their motivations to become vaccinated. At the threatening rise of the highly infectious Omicron variant, in December 2021, we interviewed people in waiting lines of vaccination centers. Our results identify risk-averse and social-distancing-compliant people as showing high confidence in the vaccine, which motivates them to receive it for reasons of protecting themselves and others. By contrast, policy incentives, such as “3G/2G” restrictions, motivate risk-tolerant people who opted for vaccination to get access to public areas. Trusting people who regularly vote are little afraid of vaccines’ side effects. Our findings offer insights for policymakers in societies and firms that help to tailor policies promoting vaccination based on people’s economic preferences.
Preoperative neutrophil-to-lymphocyte ratio correlates with PD-L1 expression in immune cells of patients with malignant pleural mesothelioma and predicts prognosis
Authors: Okita Riki , Kawamoto Nobutaka , Okada Masanori , Inokawa Hidetoshi , Yamamoto Naoki , Murakami Tomoyuki , Ikeda Eiji 
Published: March 2023

AbstractWe assessed the prognostic value of five complex inflammatory and nutritional parameters, namely neutrophil-to-lymphocyte ratio (NLR), prognostic nutritional index (PNI), C-reactive protein-to-NLR ratio (C/NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) using data from patients with malignant pleural mesothelioma (MPM) undergoing extrapleural pneumonectomy (EPP). Moreover, the correlation between these five parameters and programmed cell death protein 1 ligand-1 (PD-L1) expression in the tumor microenvironment was evaluated. This study included consecutive MPM patients who underwent EPP. The histological subtype of the eligible patients (n = 61) correlated with all five parameters. Moreover, the PD-L1 expression scores for immune cells correlated with NLR and PLR, and the PD-L1 expression scores for both tumor cells and immune cells were inversely correlated with both PNI and LMR. Univariate analysis elucidated that NLR, PNI, and C/NLR were predictors of 5-year overall survival (OS), and multivariate analysis revealed that NLR was an independent predictor of 5-year OS, suggesting that NLR is a preoperative, prognostic factor for patients with MPM who are scheduled for EPP. To the best of our knowledge, this is the first study to evaluate the prognostic potentials of NLR, PNI, C/NLR, PLR, and LMR simultaneously in patients with MPM who underwent EPP.
Mechanical, microstructural and fracture studies on inconel 825–SS316L functionally graded wall fabricated by wire arc additive manufacturing
Authors: Senthil T. S. , Babu S. Ramesh , Puviyarasan M. 
Published: March 2023

AbstractThis paper presents a novel method that uses the cold metal transfer based wire arc additive manufacturing process to fabricate functionally graded Inconel 825–SS316L walls. The optical micrograph of Inconel 825 exhibits continuous and discontinuous dendritic structures. The SS316L region comprises 5% of δ-ferrite in primary austenitic (γ) dendrites which was confirmed by the Creq/Nieq ratio of 1.305. The functionally graded interface reveals a partially mixed zone with a transition from the elongated dendrites to fine equiaxed dendrites. The tensile properties of the fabricated wall were determined at room temperature using specimens extracted from Inconel 825, SS316L, and the interface regions. The morphology of the tensile tested specimens revealed significant plastic deformation, indicating ductile failure. The fracture toughness of the wall was experimentally investigated by employing the crack tip opening displacement (CTOD) test. The fracture morphology exhibited a ductile mode of fracture with striations perpendicular to the direction of crack development. Elemental mapping revealed that there was no evidence of elemental segregation on the fractured surfaces, and the elements were uniformly dispersed. The CTOD measures 0.853 mm, 0.873 mm on the Inconel 825 side and the SS316L side respectively. The test results confirm that both the Inconel 825 and SS316L sides have good fracture toughness.
Spherical alveolar shapes in live mouse lungs
Authors: Kim Min Woo , Weon Byung Mook , Je Jung Ho 
Published: March 2023

AbstractUnderstanding how the alveolar mechanics work in live lungs is essential for comprehending how the lung behaves during breathing. Due to the lack of appropriate imaging tools, previous research has suggested that alveolar morphologies are polyhedral rather than spherical based on a 2D examination of alveoli in fixed lungs. Here, we directly observe high-resolution 3D alveoli in live mice lungs utilizing synchrotron x-ray microtomography to show spherical alveolar morphologies from the live lungs. Our measurements from x-ray microtomography show high sphericity, low packing density, big alveolar size, and low osmotic pressure, indicating that spherical alveolar morphologies are natural in living lungs. The alveolar packing fraction is quite low in live lungs, where the spherical alveoli would behave like free bubbles, while the confinement of alveolar clusters in fixed lungs would lead to significant morphological deformations of the alveoli appearing polyhedral. Direct observations of the spherical alveolar shapes will help understand and treat lung disease and ventilation.
Elevated number and density of macrophage-like cell as a novel inflammation biomarker in diabetic macular edema
Authors: Wang Zongyi , An Haiyan , Tang Jiyang , Jin Enzhong , Li Siying , Zhang Linqi , Huang Lvzhen , Qu Jinfeng 
Published: March 2023

AbstractTo quantitatively analyze the number and density of macrophage-like cells (MLCs) at the vitreoretinal interface at macular region in diabetic retinopathy (DR) with and without diabetic macular edema (DME). This cross-sectional study involved 240 eyes of 146 treatment-naïve DR patients, including 151 eyes with DME. The number and density of MLCs were analyzed quantitatively using optical coherence tomography angiography (OCTA) and were compared between DME and non-DME eyes as well as proliferative DR (PDR) and non-PDR (NPDR) eyes. Correlation between MLCs density and vessel density of macular superficial capillary plexus (SCP) at macular region was evaluated. The number and density of macular MLCs were both elevated in DME group compared to non-DME group (all p < 0.001). The morphology of MLCs in DME eyes appeared larger and fuller. NPDR eyes had higher number and density of MLCs (p = 0.027 and 0.026), greater central macular thickness (CMT) (p = 0.002) and vessel density than PDR eyes in non-DME group but comparable to PDR eyes in DME group. The number and density of MLCs at macular region were significantly higher with larger and fuller morphology in DR patients with DME than those without DME. PDR eyes had fewer MLCs than NPDR eyes for DR eyes without DME.
Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis
Authors: Zhu Yu , Yu Juan , Zhu XinHua , Yuan JiaSheng , Dai MeiNa , Bao YouWei , Jiang YinLi 
Published: March 2023

AbstractThe present study aims to investigate the effect of immunotherapy in a mouse model of allergic rhinitis (AR) and to explore the possible molecular mechanisms of action. An animal model of AR was established by sensitization and challenge of BALB/c mice with house dust mite (HDM) extract. The mice were injected subcutaneously with HDM for immunotherapy. AR nasal symptoms were evaluated according to the frequencies of nose rubbing and sneezing and the degree of rhinorrhea. The nasal mucosa and lung tissue architecture and inflammatory status by histological analysis; the infiltration of eosinophils in nasal lavage fluid (NALF) of mice was observed by Diff-Quik stain; ELISA-based quantification of serum HDM-specific IgE and TH1/TH2 cytokine concentration; and flow cytometry detected the number of serum CD4+/CD8+ cells to evaluate the mechanism of immunotherapy. It was found that after immunotherapy, the AR symptom score was reduced, the number of eosinophils in NALF was reduced, and the infiltration of inflammatory cells and tissue damage in the nasal mucosa and lung tissue were alleviated. Immunotherapy can increase the number of CD4+ T cells in the peripheral blood, increase the ratio of CD4+/CD8+ cells, increase the expression of Th1 cytokines such as IL-2 and IFN-γ, reduce the expression of Th2 cytokines such as IL-4 and IL-5. The results showed that repeated intraperitoneal injection of crude extract of HDM for sensitization, followed by nasal drops can effectively construct a mouse model of AR, and subcutaneous injection of immunotherapy in mice can reduce allergic inflammation in model mice and improve the inflammatory infiltration of the nasal cavity in allergic rhinitis. Immunotherapy can reduce the expression of inflammatory factors in AR, improve Th1/Th2 balance, and may play a role in the treatment of AR by improving the function of immune cells.
Changes in failure to rescue after gastrectomy at a large-volume center with a 16-year experience in Korea
Authors: Park Sung Hyun , Kim Ki-Yoon , Cho Minah , Kim Yoo Min , Hyung Woo Jin , Kim Hyoung-Il 
Published: March 2023

AbstractFailure to rescue (FTR), the mortality rate among patients with complications, is gaining attention as a hospital quality indicator. However, comprehensive investigation into FTR has rarely been conducted after radical gastrectomy for gastric cancer patients. This study aimed to assess FTR after radical gastrectomy and investigate the associations between FTR and clinicopathologic factors, operative features, and complication types. From 2006 to 2021, 16,851 gastric cancer patients who underwent gastrectomy were retrospectively analyzed. The incidence and risk factors were analyzed for complications, mortality, and FTR. Seventy-six patients had postoperative mortality among 15,984 patients after exclusion. The overall morbidity rate was 10.49% (1676/15,984 = 10.49%), and the FTR rate was 4.53% (76/1676). Risk factor analysis revealed that older age (reference: < 60; vs. 60–79, adjusted odds ratio [OR] 2.07, 95% confidence interval [CI] 1.13–3.79, P = 0.019; vs. ≥ 80, OR 3.74, 95% CI 1.57–8.91, P = 0.003), high ASA score (vs. 1 or 2, OR 2.79, 95% CI 1.59–4.91, P < 0.001), and serosa exposure in pathologic T stage (vs. T1, OR 2.74, 95% CI 1.51–4.97, P < 0.001) were associated with FTR. Moreover, patients who underwent gastrectomy during 2016–2021 were less likely to die when complications occurred than patients who received the surgery in 2006–2010 (OR 0.35, 95% CI 0.18–0.68, P = 0.002). This investigation of FTR after gastrectomy demonstrated that the risk factors for FTR were old age, high ASA score, serosa exposure, and operation period. FTR varied according to the complication types and the period, even in the same institution.
The protease calpain2a limits innate immunity by targeting TRAF6 in teleost fish
Authors: Chen Yang , Wang Pengfei , Li Qi , Yan Xiaolong , Xu Tianjun 
Published: March 2023

AbstractTNF receptor-associated factor 6 (TRAF6) plays a key signal transduction role in both antibacterial and antiviral signaling pathways. However, the regulatory mechanisms of TRAF6 in lower vertebrates are less reported. In this study, we identify calpain2a, is a member of the calcium-dependent proteases family with unique hydrolytic enzyme activity, functions as a key regulator for antibacterial and antiviral immunity in teleost fish. Upon lipopolysaccharide (LPS) stimulation, knockdown of calpain2a promotes the upregulation of inflammatory cytokines. Mechanistically, calpain2a interacts with TRAF6 and reduces the protein level of TRAF6 by hydrolyzing. After loss of enzymatic activity, mutant calpain2a competitively inhibits dimer formation and auto-ubiquitination of TRAF6. Knockdown of calpain2a also promotes cellular antiviral response. Mutant calpain2a lacking hydrolase activity represses ubiquitination of IFN regulatory factor (IRF) 3/7 from TRAF6. Taken together, these findings classify calpain2a is a negative regulator of innate immune responses by targeting TRAF6 in teleost fish.
Fenestrated Anterior Communicating Artery Complex Mimicking an Unruptured Aneurysm: Diagnostic Pitfall
Authors: Tsukada Atsushi , Yanaka Kiyoyuki , Takeda Hayato , Onuma Kuniyuki , Takada Maya , Nakamura Kazuhiro , Ishikawa Eiichi 
Published: March 2023

AbstractAnatomical variations often occur in the anterior communicating artery (AComA) complex, and a careful preoperative evaluation is required before repair of this lesion. We report a case of a fenestrated AComA complex mimicking an unruptured cerebral aneurysm. A 49-year-old woman was referred to our hospital under suspicion of unruptured aneurysms of the AComA and the left middle cerebral artery on magnetic resonance angiography (MRA). Additional three-dimensional computed tomographic angiography (CTA) showed the lesion arising from the AComA complex with a maximum diameter of 4.2 mm. Intraoperative findings showed that the putative aneurysm was actually a fenestrated AComA complex as the blood vessels that formed the AComA complex were dilated and meandering. After the operation, MRA and CTA three-dimensional images were reviewed again but we could still not diagnose the lesion as a fenestrated AComA complex rather than an aneurysm. However, in the MRA source image, a secant line in the lesion was the only finding suggestive of a fenestration. The AComA complex is often associated with various vascular malformations, and it is essential to consider this association in the preoperative evaluation. The interpretation of source images may be helpful for accurate diagnosis and surgical planning.
Low-Grade Astrocytoma Causing Dural and Calvarial Destruction
Authors: Belfquih Hatim , Slioui Badr , Azami Mohamed Amine , Akhaddar Ali 
Published: March 2023

AbstractMost of the literature on intra-axial lesions causing calvarial and dural destruction comes from case reports for glioblastoma, lymphoma, metastasis, and aggressive meningioma. Destruction of dura and calvaria by low-grade gliomas is extremely uncommon; cases reported so far have been mostly oligodendrogliomas. This article describes the unusual case of a 23-year-old male patient with a left-sided intra and extracranial tumor involving the frontal lobe, destroying the overlying dura and calvaria, who underwent maximal safe resection. Histopathology showed the tumor to be a low-grade astrocytoma. The calvarial thinning or remodeling caused by low-grade gliomas is thought to result from their chronic mass effect, by displacing the overlying layer of cerebrospinal fluid and transmitting brain pulsations directly to the inner table of the skull. Pressure thinning of the inner table of the skull may be caused by Pacchionian granulations close to the midline. Although this is extremely uncommon, magnetic resonance imaging may include low-grade astrocytoma in the differential diagnosis in such cases.
Complementarity between quantum coherence and mixedness: a majorization approach
Authors: Alvarez Yanet , Losada Marcelo , Portesi Mariela , Bosyk Gustavo M 
Published: March 2023

Abstract
Quantum coherence is a relevant resource for various quantum information processing tasks, but it is fragile since it is generally affected by environmental noise. This is reflected in the loss of purity of the system, which in turn limits the amount of quantum coherence of it. As a consequence, a complementarity relation between coherence and mixedness arises. Previous works characterize this complementarity through inequalities between the ℓ
1-norm of coherence and linear entropy, and between the relative entropy of coherence and von Neumann entropy. However, coherence–mixedness complementarity is expected to be a general feature of quantum systems, regardless of the measures used. Here, an alternative approach to coherence–mixedness complementarity, based on majorization theory, is proposed. Vectorial quantifiers of coherence and mixedness, namely the coherence vector and the spectrum, respectively, are used, instead of scalar measures. A majorization relation for the tensor product of both vectorial quantifiers is obtained, capturing general aspects of the trade-off between coherence and mixedness. The optimal bound for qubit systems and numerical bounds for qutrit systems are analyzed. Finally, coherence–mixedness complementarity relations are derived for a family of symmetric, concave and additive functions. These results provide a deeper insight into the relation between quantum coherence and mixedness.
II International scientific and practical conference “Improving energy efficiency, environmental safety and sustainable development in agriculture” (EESTE-II-2022)
Authors: Sadullozoda Shahriyor , Gibadullin Arthur 
Published: March 2023

Abstract
Within the framework of the presented material, the results of the conducted II International scientific and practical conference “Improving energy efficiency, environmental safety and sustainable development in agriculture” (EESTE-II-2022).
List of Acknowledgments, International Program Committee are available in this pdf.
Analyzing the method of increasing the energy efficiency for a direct-flow generator unit based on an educational laboratory stand
Authors: Kuskarbekova S I , Khrutskaya A D , Fedorenko E N , Karimov I F , Zulkarnaev D U 
Published: March 2023

Abstract
The article investigates the modes of gas flow through the flue of a steam direct-flow generator unit using a laboratory stand formed from a cylindrical coil. The criterion for determining the gas flow regime is a dimensionless quantity called the Reynolds number. The speeds and temperatures of the airflow were determined experimentally. Based on these results, the Reynolds number was calculated and the flow modes at each measurement point were determined, as well as the dependence of the Reynolds number on temperature and velocity was obtained, the experiment was analyzed. The reason for further study of aerodynamic processes in a ramjet steam generator is substantiated.
Ensuring the environmental friendliness of internal combustion engines of agricultural machines in conditions of limited air exchange
Authors: Sotnikova E V , Yu Kalpina N 
Published: March 2023

Abstract
The method of protection of personnel working in greenhouses, greenhouses and premises for maintenance and repair of agricultural machinery from exposure to exhaust gases in accordance with the “environmental concept”, according to which the negative impact must be reduced before the products of spent gases will enter the air of the working area of the service personnel and the driver of the machine. The problem was solved with the help of evaporative humidification of the air-fuel mixture due to its simplicity and effectiveness, confirmed during tests on a tractor.