bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo analysis of Porous Bioactive Silicon Carbide Scaffold for Craniofacial Bone Augmentation
Authors: Alfotawi Randa , Premnath Sangeetha , El-Ghannam Ahmad , Alsafadi Mona , Mahmood Amer 
Published: July 2023

Abstract

Bone augmentation is a vital area of research because of its high clinical demand and the reported complications associated with the available biomaterials.
Purpose: The study assess the role of decellurized skeletal muscle (DSM) when combined with synthesized porous bioactive silicon carbide (SiC) ceramic and evaluated its ability to augment calvarious bone at rat`s model. Material&
Methods: Eighteen rats were divided into two groups; Group 1 (n=9), SiC discs (10 mm x 0.2 mm) treated with 20% NaOH were placed as an onlay grafts on calvarial bone. Meanwhile, in Group 2 (n=9), SiC discs (10 mm x 0.2 mm)were covered with DSM. After 12 weeks, the grafted tissues were harvested and examined using cone-beam computed tomography (CBCT), mechanical testing, and histological analysis.
Results: CBCT showed more radio-opacity for the remnant of SiC compared to native bone was noted in group2 at surface area and volume o at 2.48mm2+/- (Sd=1.6) and 14.9+/-(Sd=7.8 )mm3 respectively. The estimated quantitative average surface area of the radio-opacity for group 1 and volume were 2.55 mm2+/- (Sd=3.7) and 11.25+/-(Sd=8.9), respectively. Mechanically, comparable values of the flexural strength and statistically significant higher modulus of elasticity of calvaria in Group 1 compared to Group 2 and control (P < 0.001). Histologically, group2 region of woven bone was seen close to the lamellar bone (native bone), and there was immature bone present near the implanted SiC.
Conclusion: The tested construct made of SiC/DSM has potential to osteointegrate into native bone, making it a suitable material for bone augmentation.
Prevalence and Risk Factors of Campylobacter Species from Cow Milk in Oromia Region, Ethiopia
Authors: Eshetu Adane , Sisay Tesfaye , Adimassie Abera , Vipham Jessie L. , Kovac Jasna , Zewdu Ashagrie 
Published: July 2023

Abstract
Campylobacter is one of the major causes of gastroenteritis and is commonly transmitted through the consumption of raw milk or improperly pasteurized milk. A cross-sectional study was conducted from January 2019 to March 2020 in four study sites in the Oromia region of Ethiopia to isolate, identify, and estimate the prevalence of Campylobacter species in milk samples and to determine their antibiotic susceptibility pattern. A total of 384 milk samples were randomly collected from raw milk (n = 192) from farmers and collectors and pasteurized milk (n = 192) from processors and retailers, and the isolation and identification of Campylobacter spp. were performed using standard bacteriological techniques and PCR. Of the total 384 milk samples, 35 (9.1%) were found positive for Campylobacter spp. The highest prevalence of Campylobacter spp. was found in collector raw milk (13.5%), followed by farmer raw milk (12.5%), and pasteurized milk (5.2%). The most prevalent Campylobacter spp. isolated from milk samples was C. jejuni (100%). The overall prevalence of Campylobacter in dairy value chains, including producer, collector, processor, and retailer, was 12.5%, 13.5%, 5.2%, and 5.2%, respectively. Cold storage, material type for making collection rooms, calibrating the pasteurizer machine, restricting milk handlers that are sick, means of transportation, and maintaining temperature during transportation had a statistically significant association. 100% and 8.6% of the Campylobacter isolates were sensitive to ciprofloxacin and chloramphenicol, respectively. However, all of the isolates were resistant to ampicillin, clindamycin, ox tetracycline, and trimethoprim. Moreover, 80% of the C. jejune were resistant to tetracycline and streptomycin. 26% of the species developed ciprofloxacin degradation.
Hence, there is a chance of acquiring infection via the consumption of raw or undercooked milk. Thus, the implementation of hygienic practices from the producer to the retailer's market, proper handling to avoid cross-contamination and proper pasteurization is very important in preventing Campylobacter infection.
Bone mineral density and muscle mass associated with healthy eating index in postmenopausal women; Results from RaNCD cohort study
Authors: Kamari Negin , Fateh Hawal Lateef , Pasdar Yahya , Rezaeian Shahab , Shakiba Ebrahim , Najafi Farid 
Published: July 2023

Abstract
Background
The Healthy eating index 2015 (HEI-2015) is a tool for checking the quality of diet. This index is used to examine how well people's dietary behavior fits certain criteria for achieving a healthy diet in Dietary Guidelines for Americans 2015–2020. We look at the possible association between the HEI-2015 and bone mineral density (BMD) and muscle strength in postmenopausal women.
Methods
This research included 1012 postmenopausal women from the recruitment phase of the Ravansar Noncommunicable Diseases (RaNCD) cohort study in Kermanshah-Iran. A standardized and repeatable food-frequency questionnaire (FFQ) that contains 118-item used to quantify dietary consumption. Anthropometric indices measured via Bio-Impedance Analyzer BIA (Inbody 770, Inbody Co, Seoul, Korea).
Result
The mean age of postmenopausal women was (56.41 ± 5.31) years. Participants in the highest quartile had a mode daily energy intake compared to the lowest quartile (2329.89 ± 837.59), (P < 0.001). Women in the upper quartiles had greater Skeletal Muscle Mass (SMM) than those in the lower quartiles (21.61 ± 2.80 vs 20.52 ± 3.13; p = 0.0002). The linear model results demonstrated a strong positive relationship between HEI score and greater BMD (P = 0.0014).
Conclusion
A diet of high quality followed by a high HEL-2015 score was shown to be strongly connected to increased BMD and muscle mass in Kurdish postmenopausal women.
Diagnostic value of vascular endothelial growth factor (VEGF) levels in gastrointestinal cancers with ascites – A cross sectional study
Authors: Samuel Evangeline Mary Kiruba , Sundaramurthi Sudharsanan , Hanumanthappa Nandeesha , NelamangalaRamakrishnaiah Vishnu Prasad 
Published: July 2023

Abstract
Introduction
Malignant ascites is suggestive of peritoneal carcinomatosis. The distinction between a malignant and non-malignant ascites in a patient with malignancy is important as it alters the management and prognosis. Current diagnostic methods are imaging, cytology and diagnostic laparoscopy, all of which have low sensitivities. The Vascular Endothelial Growth Factor (VEGF) is essential for tumour growth and hence, ascitic VEGF levels can be a method of diagnosis of malignant ascites.
Methods
This cross-sectional study was conducted in patients with gastrointestinal malignancies and ascites. The calculated sample size was 68 patients, who were divided into those who were truly positive and negative for malignant ascites based on a composite gold standard, comprising of cytology, CECT and laparoscopy. The ascitic VEGF levels in these patients were compared.
Results
A total of 84 patients were enrolled, out of which 60.71% were found to have truly malignant ascites. It was found that greater the volume of ascites, greater the statistical likelihood of finding truly malignant ascites. The ascitic VEGF levels had a non-normal distribution, with the median value being 783.64 and 41.12 pg/mL for malignant and non-malignant ascites(p < 0.001). Using a ROC Curve, a cut-off of 83.68pg/mL was obtained, with a sensitivity of 100% and a specificity of 93.94%.
Conclusion
This study demonstrates that ascitic VEGF levels is significantly elevated in patients with gastrointestinal malignancies and malignant ascites and hence can reliably be used for diagnosing malignant ascites. This study also shows that massive ascites and well-differentiated tumours have a higher rate of peritoneal carcinomatosis.
Magnetogravitational instability in strongly coupled rotating clumpy molecular clouds including heating and cooling functions
Authors: Dhiman Joginder Singh , Mahajan Mehak 
Published: July 2023

Abstract
The molecular clouds (MCs) formation is caused due to gravitational collapse mechanism and is significantly affected due to radiative heating and cooling processes. The paper analyzes the gravitational instability in the strongly coupled clumpy MCs, under the effects of uniform rotation, magnetic field, and heat-loss functions. The generalized hydrodynamic (GHD) equations coupled with the modified energy equation (which incorporates the heating and cooling effects due to cloud-cloud collisions) are used to describe the mathematical model. A general dispersion relation is analytically derived using the normal mode analysis method, by taking the orientations of the magnetic field and uniform rotation along the vertical direction. The Jeans criteria of gravitational instability are obtained for strongly and weakly coupling limits, which are modified due to the Coulomb coupling parameter, heat-loss functions, and viscoelastic coefficients. It is found that the value of the critical wavenumber decreases due to the strong coupling between the plasma particles (coupling parameter) and clump stirring processes (heating rate), thus both have stabilizing influence on the onset of gravitational collapse in clumpy MCs. The influence of various parameters on the growth rate of the instability is discussed numerically and it is found that the cooling rate parameter which described the cloud-cloud collisions shows the destabilizing effect. The region of instability is observed to be smaller in the strongly coupled clumps (kinetic limit) than in the weakly coupled (hydrodynamic limit) clumps. The results are helpful in understanding the role of heating and cooling mechanisms in the MCs formation.
A Comparative Study of Deterministic and Uncertain Area Coverage Strategies in WSNs
Authors: Boualem Adda , Runz Cyril , Ayaida Marwane 
Published: July 2023

Abstract
The advent of sensor networks offers the opportunity to track events, monitor areas, and prevent withnodes barriers any intrusion type in deterministic and uncertainenvironments. This paper presents a comparative study ofour proposed strategies to address deterministic and uncertaincoverage models. Consequently, the connectivity and lifetime toaddress current and future challenges of coverage in WSNs. Thisstudy provides a comparative case focusing on our works, (a)based on deterministic models, and on (b) uncertainty models,showing the shortcomings, strengths and weaknesses of eachmodel. Finally, in present and forthcoming new technologies, asome avenues for exploring coverage problems are cited.
General hyperstability criteria for Jensen-type equations
Authors: Dăianu Dan Marceliu 
Published: July 2023

Abstract

We provide two large classes of control functions that ensure the hyperstability of Jensen-type equations on restricted domains. Among other consequences, we obtain improvements on similar results known in the literature for some Aoki-Rassias-type control functions and hyperstability results for some equations whose solutions are Jensen functions compounded with radical-type functions or with trigonometric functions.
Mathematics Subject Classification (2010). Primary 39B82, 39B52; Secondary 39A70, 39B62, 39A10.
Pulsational Studies of δ Scuti Stars with TESS and K2: Discovery of New Variables and Insights into Stellar Structure
Authors: Zhou Ai-Ying 
Published: July 2023

Abstract
With TESS and Kepler K2 light curve archive, 13 known $\delta$ Scuti stars were reanalyzed and a small region survey of 1 deg radius centered on V1821 Cyg in the open cluster NGC 26871 was carried out. We discovered 45 new variables which include: 11 eclipsing binaries with pulsating components, 9 $\gamma$ Doradus stars, 8 $\delta$ Scuti stars, and other types. AD Ari is now reclassified as an eclipsing binary. The strong beating caused by two close frequencies present in the $\delta$ Sct star CD-54 7154. We can resolve at least 53 pulsation frequencies in $\iota$ Boo. At the same time, V1821 Cyg, V2238 Cyg and other stars pulsate with a couple of dozen frequencies, which is a significant improvement from the previous ground photometry results of a few. In particular, K2 data greatly increased the number of pulsation frequencies of three $\delta$ Sct stars: BU and BV from six to 27, and BN Cnc from five to ten. EX Cnc is an enticing asteroseismic target for its three groups of pulsation frequencies. Only a few stars can exhibit rich and regular pulsation patterns in their frequency spectra. With high-precision and long-term space-borne photometry, we can discern the pulsational contents of these stars more clearly and enhance our knowledge of them. The true nature of a star’s variability can be revealed by advanced photometry. The current small survey deserves to be continued and further expanded. The H--R diagram using the largest amount of member stars shows both $\delta$ Scuti and $\gamma$ Doradus stars occupy almost the same region; this would suggest a united pulsation mechanism.
Unveiling the therapeutic potential of Ponatinib and Imatinib against Aurora Kinase A in gastric cancer cells
Authors: Varshney Nidhi , Kashyap Dharmendra , Saini Vaishali , Singh Siddharth , Kumar Sachin , Elangovan Manivannan , Brás Natércia F. , Jha Hem Chandra 
Published: July 2023

Abstract
Aurora kinase A (AURKA) is considered a promising therapeutic target because of its abnormal expression pattern in multiple cancers. Since AURKA possesses a high similarity in active binding site with other kinases, we attempted to investigate and rationalize several kinase inhibitors that are currently under preclinical and clinical settings. All such inhibitors were selected based on sequence and structural similarity of AURKA with other kinases. Molecular docking revealed that Ponatinib, Imatinib, GSK-2334470, CFI-400945, MK2 inhibitor 3, and Vemurafenib bound to AURKA with high affinity. Molecular Dynamic (MD) simulations were carried out to assess pocket druggability, and stability of the potential inhibitors with respect to binding free-energy calculations. The findings suggested stable binding, particularly, for Ponatinib and Imatinib with AURKA via Van der Waals interactions. We identified Leu139, Val147, Lys162, Tyr212, Gly216, Leu263, and Asp274 as hotspot residues for binding of the inhibitors. Further, in vitro analyses of Ponatinib and Imatinib along with positive control MLN8237 realized decrease in AURKA expression at genomic as well as protein level studies even after ectopic transfection of AURKA. Notably, these compounds also reduced the expression of related signaling molecules β-catenin, STAT3 and its phosphorylation and NF-𝜿B and its phosphorylation. Beside the negative influence exercised by inhibitors on the oncogenic effect such as cell proliferation and genomic instability, Ponatinib and Imatinib also induced apoptosis rescue by AURKA and enhanced the protein expression of apoptotic markers like Caspase 9, 8, 3 and PARP1. Our results suggest these compounds as promising molecules to be used against AURKA.
Translation and Validation of a Chinese Version of the Body Talk Scale for Women and Men
Authors: He Jinbo , Lu Yining , Barnhart Wesley R. , Tang Chanyuan , Zhang Hengyue , Zhao Yiqing , Lin Linda 
Published: July 2023

Abstract
Research on body talk has received increasing research interest in recent years, with accumulating evidence supporting the link between body talk and body image and eating disturbances. However, research on body talk in China is still relatively scarce and is generally focused on fat talk, especially for women, and much remains unknown about muscle talk and positive body talk for both Chinese women and men. To promote a better understanding of body talk in the Chinese context, the present study aimed to translate the Body Talk Scale (BTS) into Chinese Mandarin (i.e., C-BTS) and evaluate the factor-structure and psychometric properties of the C-BTS in Chinese adult women and men. The English version of the BTS was translated into Chinese Mandarin with standard procedures. With 300 Chinese women (Mage = 29.48 years, SD = 7.26) and 300 men (Mage = 29.36 years, SD = 6.81), we examined the factor structure and gender invariance of the C-BTS, as well as internal consistency reliability, test-retest reliability, and construct validity including convergent, concurrent, and incremental validity of the C-BTS. The results indicated that the C-BTS had three subscales (Negative Fat Talk, Negative Muscle Talk, and Positive Body Talk) and had good reliability and validity. The findings demonstrate that the C-BTS is a useful measure of body talk in both Chinese women and men.
Experiences of healthcare personnel on the efficacy of artemisinin-based combination therapies and malaria diagnosis in hospitals in Uganda
Authors: Ocan Moses , Bakubi Racheal , Tayebwa Mordecai , Basemera Joan , Nsobya Sam 
Published: July 2023

Abstract
Background
The risk of widespread resistance to artemisinin-based combination therapies (ACTs) remains high in Uganda following detection of Plasmodium falciparum parasites with delayed Artemisinin clearance genotype and phenotype. Establishment of context specific interventions to mitigate emergence and spread of artemisinin resistance is thus key in the fight against malaria in the country. The aim of this study was to explore the experiences of healthcare personnel on malaria diagnosis and self-reported efficacy of ACTs in management of malaria symptomatic patients in hospitals in low and high malaria transmission settings in Uganda.
Methods
This was a qualitative study in which data was collected from healthcare personnel in hospitals using key informant interviews. The key informant interview guide was developed, pre-test prior to use and covered the following areas, (i) sociodemographic characteristics, (ii) malaria diagnosis (clinical and parasite based), (iii) quality assured artemisinin-based combination therapy, (iv) malaria patient follow-up, (v) artemisinin resistance, (vi) antimalarial self-medication. Data was entered in Atlas.ti ver 9.0 and analysis done following a framework criterion.
Results
A total of 22 respondents were interviewed of which 16 (72.7%) were clinicians. Majority, 81.8% (18/22) of the respondents were male. The following themes were developed from the analysis, malaria diagnosis (procedures and challenges), use of malaria laboratory test results, malaria treatment in hospitals, use of quality assured ACTs (QAACTs) in malaria treatment, and efficacy of ACTs in malaria treatment.
Conclusion
Most healthcare personnel-initiated malaria treatment after a positive laboratory test. Malaria patients who report remaining symptomatic after taking a full course of ACTs are common especially in high malaria transmission settings in Uganda. There is need for regular monitoring of artemisinin resistance emergence and spread in the country.
Demystification of CLSPN multiple potentialities in hepatocellular carcinoma: insight into individualized diagnosis and precision therapeutics
Authors: Shi Yanlong , Wang Yizhu , Niu Kaiyi , Zhang Wenning , Zhang Yewei 
Published: July 2023

Abstract
CLSPN, an essential molecule of the S-phase checkpoint in DNA replication stress, have not been elucidated in hepatocellular carcinoma (HCC). In this study, we firstly discovered and systematically verified CLSPN expression using RT-qPCR and western blotting assay, and its high expression was an independent prognostic factor in HCC. Patients with CLSPN low-expression had higher infiltration levels of T cell CD4 + memory resting, monocyte, mast cell activated, dryness index and lower immune response in HCC. Then, CLSPN silencing inhibited the proliferation, migration, invasion and cell cycle progression of HCC cells proved by CCK-8, transwell and cell cycle assay. We established a key lncRNA PSMA3-AS1/hsa-miR-101-3p/CLSPN regulator axis in HCC. Furthermore, CLSPN-mediated ubiquitination or deubiquitination may regulate post-transcriptional modifications in HCC. The emerging CLSPN potentialities might be mediated by the β-catenin-mediated Wnt signaling pathway. In addition, we detected CLSPN interaction protein profile, which further confirmed CLSPN involved in posttranscriptional modification, protein turnover, and biogenesis locating in cytoplasm, secreted, and mitochondrion. Therefore, it was the first time to discover and verify expression, prognosis, immunotherapy, RNAs regulator, posttranscriptional modification, and molecular mechanisms of CLSPN in HCC. These novel insights might accelerate the process of individualized diagnosis and precision therapeutics for patients with HCC.
mRNA sequencing provides new insights into the pathogenesis of Hirschsprung's disease in mice
Authors: Yang Qiwen , wang fuwen , Wang Zhaofei , Guo Jiajun , Chang Tingjin , Dalielihan Baligen , Yang Ge , Lei Chuzhao , Dang Ruihua 
Published: July 2023

Abstract
Hirschsprung's disease (HSCR) is an intestinal development defect caused by multiple gene mutations. Under natural circumstances, the incidence in newborns is approximately 1/5000. It has been confirmed that Hirschsprung's disease is a neurocrest-derived disease, and disorders in the development and migration of neural crest cells may lead to the disease. To explore the key susceptibility genes in the development of Hirschsprung's disease, 8354 differentially expressed genes were identified by RNA sequencing in the colon tissues of EDNRBm1yzcm and wild mice, including 4346 upregulated genes and 4005 downregulated genes. Correspondingly, the results of RT–qPCR analysis showed good correlation with the transcriptome data. In addition, GO and KEGG enrichment results suggested that there were 8103 terms and 320 pathways in all DEGs. When P < 0.05, 1081 GO terms and 320 KEGG pathways reached a significant level. Finally, through the existing studies and the enrichment results of differentially expressed genes, it was determined that axon guidance and the focal adhesion pathway may be closely related to the occurrence of HSCR. This study analyzed and identified the differential genes in colonic tissues between EDNRBm1yzcm mice and wild mice, which provided new insight for further mining the potential pathogenic genes of Hirschsprung's disease.
From waste to growth: tea residue recycling through Trichoderma's solid-state fermentation for tea cuttings
Authors: Meng Zhen , Lv Zijian , Wang Xue , Zhang Jian , Bai Guoxin , Liu Hongjun , Li Rong , Shen Qirong 
Published: July 2023

Abstract
Background
Trichoderma is a widely recognized plant growth-promoting fungus (PGPF) that has been extensively utilized in various agricultural applications. However, the effects of Trichoderma on the growth of tea cuttings and the changes in the fungal community are still unclear. This study produced T. guizhouense NJAU 4742 (NJAU 4742) via solid-state fermentation using tea residue and evaluated its potential to promote the growth of tea cuttings.
Results
The optimal conditions for spore yield were determined as a 7:3 (w/w) ratio of tea residue to rice bran, a material thickness of 3 cm, an inoculum concentration of 15%, and an incubation time of 4 days, resulting in a spore count of 1.83×109 CFU/g. The application of NJAU 4742 spore products significantly increased the biomass of tea cuttings and influenced the fungal community composition. Moreover, the application of higher NJAU 4742 spore concentrations resulted in better growth performance, and the application of nursery substrate with 1×107 CFU/mL spores was the most economically viable option. Notably, among the top ten fungal genera with the highest relative abundances, Trichoderma showed a positive correlation with the fresh weight of tea cuttings, while the other nine genera exhibited a negative correlation.
Conclusions
This study showed that utilizing tea residue for solid-state fermentation of NJAU 4742 is a feasible approach, and the application of NJAU 4742 spores enhances the growth of tea cuttings by increasing the relative abundance of Trichoderma.