bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERSPECTIVE DIRECTIONS OF AGRICULTURAL CONSULTING DEVELOPMENT
Authors: Savchenko Vira , Kononenko Lesia 
Published: January 2025

This article explores the present landscape of agricultural consulting services. It aims to shed light on the current state of this field and identify promising avenues for the development of advisory services.
This study examines the current state of agricultural consulting, highlighting key trends, challenges, and opportunities. It identifies promising directions for the future development of advisory services.
Despite years of operation, agricultural advisory services in Ukraine have had limited impact on agribusiness development. An analysis of small-scale agricultural businesses has highlighted promising areas for future consulting services. Thus, the preferences of the clients of the advisory service of Kirovograd region include tax issues. In the context of the transformation of Ukraine's tax system in accordance with the National Revenue Strategy until 2030, most agribusinesses that are currently Group 4 taxpayers will be forced to switch to the general taxation system. Given the evolving tax landscape, consulting services should prioritize staying abreast of legislative changes, especially those related to the simplification of the tax system, as outlined in the National Revenue Strategy until 2030.
It is proved that when providing consultations, it is advisable to involve expert advisers specializing in accounting, taxation and reporting; given the availability of different taxation systems, choosing the optimal one for the client is an important area of consulting. It is noted that in modern conditions, agro-consulting activities require development and identification of priority areas. The author substantiates, based on the analysis of clients' requests, the prospects for the development of new areas of consulting: targeted at small agricultural entities on issues of management information support; consulting on taxation issues, formation of accounting policies, land relations, their tax consequences and accounting support. The author proves that the most promising areas of consulting are the issues of accounting policy formation, information support for cost and prime cost management, land relations, and the reflection of their consequences in accounting and reporting.
Correct Replacement of Calcified Hydrophilic Intraocular Lens Lentis M+ LS-313 MF30. A Case
Authors: Otrošinová Marianna , Novák Jan , Krásnik Vladimír 
Published: January 2025

The aim of the thesis is to present the case of a patient in whom bilateral calcification of the hydrophilic intraocular lens (IOL) Lentis M+
LS-313 MF30 (Oculentis) has developed. Due to the negative effect on visual functions, explantation and replacement of the artificial lens was necessary in both eyes.
Case Report: An overview of the available literature summarized the diagnostics, current examination methods and possibilities of the surgical solution of calcification of the bifocal hydrophilic lens Lentis M+ LS-313 MF30 (Oculentis). The specific solution is described in a case report of a patient in whom calcification of both lenses developed 6 years after implantation of the IOL. In 2015, the patient underwent uncomplicated cataract surgery of both eyes with the implantation of an artificial intraocular lens into the capsule. In September 2021,
an 82-year-old man was examined at our outpatient clinic for deterioration of visual acuity and changes in the material of the artificial IOL which were perceptible during a clinical examination, on the recommendation of a local ophthalmologist. Blurred vision predominated. A diagnosis of intraocular lens opacification was confirmed and documented using a Scheimpflug camera (OCULUS Pentacam HR) and anterior OCT (Avanti RTVue XR Optovue,). The patient was indicated for explantation and replacement of the opacified intraocular lens in the left and subsequently in the right eye- The same type of IOL was used for reimplantation with good functional results.
Conclusion: Since 2010, multifocal lens implantation has been on an upward trend worldwide. This type of MF IOL has also been used in thousands of implantations. A number of other explantations can be expected in the coming years. The optimal solution is the correct replacement of the calcified IOL with the same construction made of safer hydrophobic material.
Senyawa Anti Feedant : Mekanisme Kerja dan Perannya sebagai Senyawa Aktif Pestisida
Authors: Zuhro Fatimatuz 
Published: January 2025

Artikel ini bertujuan untuk membahas dan menyajikan materi tentang senyawa anti feeedant, mekanisme kerja, dan perannya sebagai pestisida nabati secara komprehensif berdasarkan studi referensi dari hasil penelitian beberapa ilmuwan terdahulu. Artikel dibuat dengan metode mengumpulkan data dengan cara memahami dan mempelajari teori-teori dari berbagai literatur yang berhubungan dengan senyawa anti feedant. Kemudian, teori-teori dari berbagai referensi tersebut dianalisis secara kritis dan mendalam agar dapat mendukung tujuan yang diharapkan dalam tulisan ini. Hasil analisis dari beberapa penelitian sebelumnya menunjukkan bahwa senyawa anti feedant sebagian besar diperoleh dari kelompok tanaman, yang mengandung bahan aktif berupa metabolit sekunder, yang jenisnya berbeda-beda, ditentukan oleh jenis dan organ suatu tanaman. Aplikasi senyawa anti feedant pada tanaman akan menghasilkan bau atau aroma yang akan dikenali oleh sel syaraf serangga hama, menghasilkan sinyal yang akan diteruskan ke otak, dan mengakibatkan hama berhenti dari aktivitas memakan tanaman inang. Pengujian aktivitas senyawa anti feedant dapat dilakukan dengan uji pilihan (choice test) atau pun uji tanpa pilihan (no choice test). Dan beberapa hasil penelitian terdahulu telah membuktikan tentang efektivitas senyawa anti feedant yang dikandung oleh beberapa tanaman dalam mengurangi tingkat kerusakan yang diakibatkan oleh serangga hama.
Communicating hydrocephalus following stereotactic radiosurgery for periventricular meningiomas: illustrative cases
Authors: Lasica Aleksandra B. , Tancu Cornel , Abussuud Zeid A. , Watkins Laurence D. , Grover Patrick J. 
Published: January 2025

BACKGROUND
Stereotactic radiosurgery (SRS) is a well-established option for the management of intracranial tumors, including meningiomas. Although valued for its low invasiveness and precision, it still carries a risk of complications. Communicating hydrocephalus is a serious, albeit rarely reported, complication of SRS. Here, the authors report two cases of communicating hydrocephalus following Gamma Knife radiosurgery (GKRS) for periventricular meningiomas.

OBSERVATIONS
In both reported cases, the patients experienced a sudden deterioration in their clinical condition a few months after the second GKRS for recurrent periventricular World Health Organization grade II atypical meningiomas. The patients underwent ventriculoperitoneal shunt implantation, and cerebrospinal fluid (CSF) samples revealed elevated levels of total protein and albumin. Additionally, both patients underwent multiple resections before radiation, and some of their lesions were located near the venous sinuses.

LESSONS
Though rare, communicating hydrocephalus represents a significant potential sequela of SRS for meningiomas that clinicians should be aware of. Factors such as tumor volume and location, histology, and previous irradiation or surgery can exacerbate radiation-induced damage and collectively underlie the development of hydrocephalus. The authors also speculate that the CSF protein levels can serve as a unifying marker of SRS-induced radiation damage in the form of radiation-associated tumor necrosis, radiation-induced hyperpermeability, and inflammatory response.
https://thejns.org/doi/10.3171/CASE24722
Efficient Prediction of Alternative Splice Forms Using Protein	Domain Homology
Authors: Hiller Michael , Backofen Rolf , Heymann Stephan , Busch Anke , Gläßer Timo Mika , Freytag Johann-Christoph 
Published: January 2025

Alternative splicing can yield manifold different mature mRNAs from one precursor. New findings indicate that alternative splicing occurs much more often than previously assumed. A major goal of functional genomics lies in elucidating and characterizing the entire spectrum of alternative splice forms. Existing approaches such as EST-alignments focus only on the mRNA sequence to detect alternative splice forms. They do not consider function and characteristics of the resulting proteins. One important example of such functional characterization is homology to a known protein domain family. A powerful description of protein domains are profile Hidden Markov models (HMM) as stored in the Pfam database. In this paper we address the problem of identifying the splice form with the highest similarity to a protein domain family. Therefore, we take into consideration all possible splice forms. As demonstrated here for a number of genes, this homology based approach can be used successfully for predicting partial gene structures. Furthermore, we present some novel splice form predictions with high-scoring protein domain homology and point out that the detection of splice form specific protein domains helps to answer questions concerning hereditary diseases. Simple approaches based on a BLASTP search cannot be applied here, since the number of possible splice forms increases exponentially with the number of exons. To this end, we have developed an efficient polynomial-time algorithm, called ASFPred (Alternative Splice Form Prediction). This algorithm needs only a set of exons as input.
Collection of Soluble Variants of Membrane Proteins for	Transcriptomics and Proteomics
Authors: Möller Steffen , Mix Eilhard , Blüggel Martin , Serrano-Fernández Pablo , Koczan Dirk , Kotsikoris Vasilis , Kunz Manfred , Watson Michael , Pahnke Jens , Illges Harald , Kreutzer Michael , Mikkat Stefan , Thiesen Hans-Jürgen , Glocker Michael O. , Zettl Uwe K. , Ibrahim Saleh M. 
Published: January 2025

The existence of a soluble splice variant for a gene encoding a transmembrane protein suggests that this gene plays a role in intercellular signalling, particularly in immunological processes. Also, the absence of a splice variant of a reported soluble variant suggests exclusive control of the solubilisation by proteolytic cleavage. Soluble splice variants of membrane proteins may also be interesting targets for crystallisation as their structure may be expected to preserve, at least partially, their function as integral membrane proteins, whose structures are most difficult to determine. This paper presents a dataset derived from the literature in an attempt to collect all reported soluble variants of membrane proteins, be they splice variants or shedded. A list of soluble variants is derived in silico from Ensembl. These are checked on their presence in multiple organisms and their number of membranespanning regions is inspected. The findings then are confirmed by a comparison with identified proteins of a recent global proteomics study of human blood plasma. Finally, a tool to support the identification of novel soluble variants by proteomics is provided.
Analysis and Prediction of Helix Shift Errors in Homology	Modeling
Authors: Bock Christoph , Hesser Jürgen 
Published: January 2025

High sequence identity between two proteins (e.g. > 60% is a strong evidence for high structural similarity. However, internal shifts in one of the two proteins can sometimes give rise to unexpectedly high structural differences. This, in turn, causes unreliable structure predictions when two such proteins are used in homology modeling. Here, we perform a computational analysis of helix shifts and we show that their occurrence can be predicted with statistical learning methods. Our results indicate that helix shifts increase the RMS error by factor 2.6 compared to those protein pairs without a helix shift. Although helix shifts are rare (1.6% of helices and a commensurately higher number of proteins are affected), they therefore pose a significant problem for reliable structure prediction systems. In this paper, we prototype a new approach for model quality assessment and demonstrate that it can successfully warn against helix shifts. A support vector machine trained on a wide range of sequence and structure properties predicts the occurrence of helix shifts with a sensitivity of 74.2% and a specificity of 83.6%. On an equalized test dataset, this corresponds to an accuracy of 78.9%. Projected to the full dataset, it translates to an accuracy of 83.4%. Our analysis shows that helix shift detection is a valuable building block for highly reliable structure prediction systems. Furthermore, the statistical learning based approach to helix shift detection that we employ here is orthogonal to well-established model quality assessment methods (which use geometric constraint checking or mean force potentials). Therefore, a further increase of prediction accuracy is expected from the combination of these methods.
BETTY: Prediction of beta;-Strand Type from Sequence
Authors: Zimmermann Olav , Wang Longhui , Hansmann Ulrich H.E. 
Published: January 2025

Most secondary structure prediction programs do not distinguish between parallel and antiparallel β-sheets. However, such knowledge would constrain the available topologies of a protein significantly, and therefore aid existing fold recognition algorithms. For this reason, we propose a technique which, in combination with existing secondary structure programs such as PSIPRED, allows one to distinguish between parallel and antiparallel β-sheets. We propose the use of a support vector machine (SVM) procedure, BETTY, to predict parallel and antiparallel sheets from sequence. We found that there is a strong signal difference in the sequence profiles which SVMs can efficiently extract. With strand type assignment accuracies of 90.7% and 83.3% for antiparallel and parallel strands, respectively, our method adds considerably to existing information on current 3-class secondary structure predictions. BETTY has been implemented as an online service which academic researchers can access from our website http://www.fz-juelich.de/nic/cbb/service/service.php.
Asthma and obesity increase inflammatory markers in children
Authors: Shailesh Harshita , Noor Safa , Hayati Lena , Belavendra Antonisamy , Van Panhuys Nicholas , Abou-Samra Abdul Badi , Worgall Stefan , Janahi Ibrahim 
Published: January 2025

BackgroundAsthma and obesity are both characterized by inflammation. However, the combined impact of these conditions on inflammatory mechanisms in children has not been studied extensively. To address this gap, we investigated the interaction effects of asthma and obesity on inflammation in children.MethodsThe multiplex and singleplex assays were used to measure the levels of circulating cytokines, including IL-2, IL-5, IL-10, IL-13, IL-17A, IL-22, IL-33, IFN-γ, TNF-α, and the adipokine leptin, in plasma. The study included 97 children with normal weight and asthma (NW-A), 100 children with overweight/obesity and asthma (OO-A), 100 with overweight/obesity and no asthma (OO), and 67 normal weight children and no asthma (NW). The independent effects of asthma, obesity, and their interaction effect on these inflammatory markers were assessed using multiple regression analysis.ResultsAsthma was associated with the increased expression of pro-inflammatory cytokines, including IL-2, IL-5, IL-13, IL-17A, IL-22, IL-33, and TNF-α, and reduced levels of anti-inflammatory cytokine, IL-10 and adipokine, leptin in the circulation. Overweight/obesity was also linked to increased plasma levels of IL-5, IL-17A, IL-22, IL-33, TNF-α, and leptin and decreased levels of IL-10. In addition, obesity and asthma showed a significant interaction effect on the plasma levels of IL-5, IL-10, IL-17A, IL-33, TNF-α, and leptin. However, the interaction did not result in a synergistic or additive impact on cytokines, indicating a moderating effect of obesity on inflammation in pediatric asthma.ConclusionBoth asthma and overweight/obesity were independently associated with increased expression of pro-inflammatory cytokines and decreased expression of anti-inflammatory cytokine in children. While the concurrent presence of asthma and obesity altered the inflammatory profile, it did not synergistically amplify the inflammation. These findings challenge the previous view that obesity enhances inflammation in individuals with asthma and highlight the importance of considering both conditions while treating obesity-associated asthma in children. Future studies are necessary to further explore the mechanisms that link obesity and asthma in the pediatric population.
Hypoxia signature derived from tumor-associated endothelial cells predict prognosis in gastric cancer
Authors: Wang Ruiheng , Liu Guijun , Wang Ke , Pan Zhanglei , Pei Zhihua , Hu Xijiao 
Published: January 2025

BackgroundA hypoxic metabolism environment in the tumors is often associated with poor prognostic events such as tumor progression and treatment resistance. In gastric cancer, the mechanism of how hypoxia metabolism affects the tumor microenvironment and immunotherapy efficacy remains to be elucidated.MethodsWe used the bulk-mapping method to analyze the signatures correlated with the response of immunotherapy in the single-cell dataset. Cellular, pathway, and gene were systematically analyzed in both single-cell and bulk validation datasets.ResultsThe most significant cell proportion difference between the response and non-response groups was in endothelial cells, which represent the malignant cells. VWF was specifically overexpressed in endothelial cells and was the hub gene of differential genes. EPAS1 was a VWF trans-regulated gene and highly positively correlated with VWF in expression. Knockdown experiments demonstrated that siVWF reduced the expression of VWF, EPAS1, and HIF1A, as well as the synthesis of lactate and adenosine which are indicators of hypoxic metabolism. These results suggest that the overexpression of core malign endothelial genes such as VWF drives hypoxic metabolism in tumors and creates an immunosuppressive environment that reduces the efficacy of immunotherapy. The adverse prognosis of the hypoxia signature was validated in the bulk cohort and significance was further enhanced after selecting core genes and combined survival weight scoring.ConclusionIn summary, high expression of the malignant endothelial cell driver genes VWF and EPAS1 enhances hypoxic metabolism, and malignant cell-immune cell interactions suppress the immune response. Therefore, the two core genes of hypoxic metabolism might represent potential therapeutic and predicting biomarkers for immunotherapy of gastric cancer in the future.
Evaluation of the viability of microencapsulated Trichoderma longibrachiatum conidia as a strategy to prolong the shelf life of the fungus as a biological control agent
Authors: Arias-Chavarría Luis Diego , Batista-Menezes Diego , Orozco-Cayasso Steffany , Vargas-Martínez Alejandro , Vega-Baudrit José Roberto , Montes de Oca-Vásquez Gabriela 
Published: January 2025

Trichoderma is an antagonistic fungus used commercially; however, the viability of these formulations is affected by biotic and abiotic factors. In this research, microcapsules of sodium alginate reinforced with nanocellulose and/or chitosan were developed to encapsulate T. longibrachiatum conidia and characterized by SEM, FTIR, and TGA. The viability of the microencapsulated conidia was evaluated through different temperatures (room temperature, 5°C and 37°C), as well as their in vitro antagonistic potential against Fusarium oxysporum. The formulations evaluated had encapsulation efficiencies above 92% and the microcapsules with alginate, chitosan, and nanocellulose maintained 100% viability at 37°C for 2 months. In addition, all formulations evaluated retained antagonistic ability against F. oxysporum. These findings support the use of alginate, nanocellulose and chitosan for the formulation of microcapsules to maintain the viability of T. longibrachiatum conidia over time and at different temperature conditions.
Case Report: Rare cardiovascular characteristics of tuberous sclerosis complex with novel TSC2 variant
Authors: Du Zhiqin , Ma Xiao , Li Jianhua , Yang Fang , Guo Yangfan 
Published: January 2025

BackgroundTuberous sclerosis complex (TSC) is a multisystem genetic disorder primarily characterized by the development of benign tumors in multiple organs. While cardiovascular involvement is less common than neurological or renal manifestations, it typically presents with cardiac rhabdomyomas (CRs). The co-occurrence of a bicuspid aortic valve (BAV) with TSC is exceedingly rare.Case summaryWe report the case of a 26-year-old woman with genetically confirmed TSC, harboring a novel pathogenic variant in the TSC2 gene. Cardiovascular characteristics included a history of heart valve disease, a bicuspid aortic valve, and severe aortic regurgitation. multi-system characteristics of TSC were also presented, affecting skin, brain, lung, kidney, and bone. She underwent aortic valve replacement but experienced postoperative complications, including significant pleural and pericardial effusions requiring drainage and subsequent thoracic duct ligation.ConclusionThis case expands the clinical spectrum of TSC-associated cardiovascular abnormalities, highlighting the rare association of BAV with this disorder. Our finding emphasizes the importance of considering TSC in individuals presenting with these cardiac features, as well reinforce the critical role of molecular genetic testing in confirming the diagnosis of TSC.