bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolomics strategy of Pikang oral liquid in the treatment of psoriasis
Authors: Wang Huiyun , Xie Baogang , Lan Lan , Wang Jun , Zhou Qingde , Du Xiaoyue , Jin Nan , Jiang Pan , Wang Wei , Xu Xinchang 
Published: February 2023

AbstractThis study aimed to investigate the effect of Pikang oral liquid (PK) on psoriasis and analyze its possible mechanism from the perspective of metabolism. A psoriasis‐form mouse model established using imiquimod (IMQ) was used to evaluate the anti‐psoriatic effects of PK. The serum samples were analyzed by high‐resolution nuclear magnetic resonance (1H NMR)‐based metabonomics. Nine amino acids were further quantitatively analyzed by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry (UPLC/Q‐TOF MS). This study suggested that PK treatment markedly attenuated IMQ‐induced psoriasis in a dose‐dependent manner. 1H NMR‐based multivariate trajectory analysis revealed that PK had a certain regression effect on eight differential metabolites. The quantitative results showed that PK could significantly regulate the serum levels of alanine, histidine and arginine to healthy control levels. The anti‐psoriasis mechanism of PK may be associated with the restoration of the disturbance in the amino acid metabolism, energy metabolism and lipid metabolism and so on. Quantitative results further confirmed that amino acid metabolism play an key role in the pathogenesis of psoriasis. Our investigation provided a holistic view of PK for intervention psoriasis and provided the scientific information in vivo about a clinical value of PK for psoriasis.
Prevalence and clinical predictors of spasticity after intracerebral hemorrhage
Authors: Liao Ling‐Yi , Xu Pei‐Dong , Fang Xiang‐Qin , Wang Qing‐Hua , Tao Yong , Cheng Huan , Gao Chang‐Yue 
Published: February 2023

AbstractBackgroundSpasticity is a common complication of intracerebral hemorrhage (ICH). However, no consensus exists on the relation between spasticity and initial clinical findings after ICH.MethodsThis retrospective study enrolled adult patients with a history of ICH between January 2012 and October 2020. The modified Ashworth scale was used to assess spasticity. A trained image analyst traced all ICH lesions. Multivariable logistic regression was used to examine the association between ICH lesion sites and spasticity.ResultsWe finally analyzed 304 patients (mean age 54.86 ± 12.93 years; 72.04% men). The incidence of spasticity in patients with ICH was 30.92%. Higher National Institutes of Health stroke scale (NIHSS) scores were associated with an increased predicted probability for spasticity (odds ratio, OR = 1.153 [95% confidence interval, CI 1.093–1.216], p < .001). Logistic regression analysis revealed that lower age, higher NIHSS scores, and drinking were associated with an increased risk of moderate‐to‐severe spasticity (OR = 0.965 [95% CI 0.939–0.992], p = .013; OR = 1.068 [95% CI 1.008–1.130], p = .025; OR = 4.809 [95% CI 1.671–13.840], p = .004, respectively). However, smoking and ICH in the thalamus were associated with a reduced risk of moderate‐to‐severe spasticity (OR = 0.200 [95% CI 0.071–0.563], p = .002; OR = 0.405 [95% CI 0.140–1.174], p = .046, respectively) compared with ICH in the basal ganglia.ConclusionsOur results suggest that ICH lesion locations are at least partly associated with post‐stroke spasticity rather than the latter simply being a physiological reaction to ICH itself. The predictors for spasticity after ICH were age, NIHSS scores, past medical history, and ICH lesion sites.
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations
Authors: Kavousi Heidari Maryam , Pourmadadi Mehrab , Yazdian Fatemeh , Rashedi Hamid , Ebrahimi Sayed Ali Seyyed , Bagher Zohreh , Navaei‐Nigjeh Mona , Haghirosadat Bibi Fatemeh 
Published: February 2023

AbstractSilk fibroin (SF), extracted from Bombyx mori, has unique physicochemical properties to achieve an efficient wound dressing. In this study, reduced graphene oxide (RGO)/ZnO NPs/silk fibroin nanocomposite was made, and an innovative nanofiber of SF/polyvinyl alcohol (PVA)/RGO/ZnO NPs was ready with the electrospinning technique and successfully characterized. The results of MIC and OD analyses were used to investigate the synthesized materials' antibacterial effects and displayed that the synthesized materials could inhibit growth against Staphylococcus aureus and Escherichia coli bacteria. However, both in vitro cytotoxicity (MTT) and scratch wound studies have shown that RGO/ZnO NPs and SF/PVA/RGO/ZnO NPs are not only non‐toxic to NIH 3T3 fibroblasts, but also can cause cell viability, cell proliferation, and cell migration. Furthermore, improving the synthesized nanofiber's structural properties in the presence of RGO and ZnO NPs has been confirmed by performing tensile strength, contact angle, and biodegradation analyses. Also, in a cell attachment analysis, fibroblast cells had migrated and expanded well in the nanofibrous structures. Moreover, in vivo assay, SF/PVA/RGO/ZnO NPs nanofiber treated rats and has been shown significant healing activity and tissue regeneration compared with other treated groups. Therefore, this study suggests that SF/PVA/RGO/ZnO NPs nanofiber is a hopeful wound dressing for preventing bacteria growth and improving superficial wound repair.
An Investigation into Chestnut Honeys from Artvin Province in Turkiye: Their Physicochemical Properties, Phenolic Profiles and Antioxidant Activities
Authors: Saral Özlem 
Published: February 2023

AbstractThe aim of this study was to investigate the physicochemical properties, phenolic compounds, and antioxidant activities of eight chestnut honey samples collected from Artvin province in Turkiye. The honey samples’ phenolic profiles were analyzed using reverse phase high performance liquid chromatography (RP‐HPLC). All honeys were monofloral, and the quantities of chestnut sativa pollen ranged from 56 % to 80 %. Total phenolic and flavonoid contents and ferric reducing/antioxidant power (FRAP) values were assessed. The pH and moisture values of the honeys ranged between 4.60 and 5.40 and between 17.60 % and 19.00 %, respectively. Electrical conductivity (EC) and Hunter color L values ranged between 0.56 and 1.12 mS/cm and between 43.16 and 67.60, respectively. Proline values ranged from 876 to 1246 mg/kg. The diastase activities of all honeys were high, ranging between 18.28 and 26.30 DU. The mean of total phenolic, total flavonoid and total antioxidant (FRAP) levels in the samples were 72.79±0.03 mg GAE/100 g, 2.25±00.03mg QE/100 g, and 312.67±2.85 μmol FeSO4/100 g, respectively. Almost all of the 19 phenolic standards studied were detected in the samples, with catechin, chrysin, caffeic acid, caffeic acid phenethyl ester (CAPE) and gallic acid being determined as major components. In conclusion, the honeys from the Artvin region were high‐quality chestnut honeys, with high polyphenolic contents and diversity and high apitherapeutic potentials.
Kinetic Protection of a Water‐Soluble Olefin Metathesis Catalyst for Potential Use under Biological Conditions
Authors: James Catriona C. , Laan Petrus C. M. , de Bruin Bas , Reek Joost N. H. 
Published: February 2023

AbstractOlefin metathesis catalysts like AquaMet are vulnerable to different decomposition pathways under biologically relevant conditions. Currently, stabilizing strategies are focused on approaches with limited relevance for application under biologically relevant conditions. Initial attempts to stabilise AquaMet by encapsulation within a supramolecular metallocage showed that the nitrate counterions of the cage improve the activity of the catalyst. We show that the chloride ligands of AquaMet can be replaced with nitrates by simple anion‐exchange. Catalytic studies into metathesis of a diallyl substrate showed that the presence of nitrate generates higher yields of the ring‐closed product compared to AquaMet alone, under aqueous and biological conditions. Kinetic studies support that the nitrate‐containing catalyst both initiates faster and performs catalysis at a much faster rate than AquaMet, while the rate of catalyst deactivation was similar. This new strategy of kinetic protection of a transition metal catalyst may have future applications for other catalytic reactions applied in vivo.
Construction of Y‐Doped BiVO4 Photocatalysts for Efficient Two‐Electron O2 Reduction to H2O2
Authors: Dai Dujuan , Bao Xiaolei , Zhang Qianqian , Wang Zeyan , Zheng Zhaoke , Liu Yuanyuan , Cheng Hefeng , Dai Ying , Huang Baibiao , Wang Peng 
Published: February 2023

AbstractPhotocatalytic hydrogen peroxide (H2O2) production on BiVO4 photocatalysts using water and oxygen as raw materials is a green and sustainable process. However, the photocatalytic efficiency of pristine BiVO4 is limited by severe charge recombination. In this work, rare earth element Yttrium (Y) doped BiVO4 photocatalysts were fabricated by the hydrothermal method. In the photocatalytic H2O2 production experiment, the optimized Y‐doped BiVO4 photocatalyst produced 114 μmol g−1 h−1 of H2O2 under simulated sunlight (AM1.5) irradiation, which is four times higher than production activity of pure BiVO4 (26 μmol g−1 h−1). Density functional theory (DFT) calculation revealed that Y doping can enhance oxygen adsorption on the BiVO4 photocatalyst surface. Mechanistic investigations suggest that the doping process induces the in situ formation of monoclinic/tetragonal BiVO4 heterojunction, which further promotes the photogenerated carriers separation efficiency.
Tailoring Galactose Oxidase for Self‐Powered Benzyl Alcohol Sensing
Authors: Chen Hongyu , Liu Weisong , Chang Lijing , Kang Zepeng , Yang Yanqin , Zhang Lingling 
Published: February 2023

AbstractBenzyl alcohol (BnOH) is a widely‐used preservative in a variety of cosmetics, but the excess addition (≥1.0 %) may cause strong symptoms such as nausea, gastrointestinal irritation, convulsion, even death, making it crucial to monitor and control the addition quantity. Herein, we have developed a test‐strip‐like BnOH detection method via tailoring a galactose oxidase (GOase) towards BnOH oxidation and preparing a self‐powered electrochromic strip for BnOH concentration visualization. A double‐substituted GOase variant (Y329S/R330F), on the basis of the reported GOase M1, has been obtained by semi‐rational design with a 24.6‐fold improved activity towards BnOH compared to GOase M1. The GOase Y329S/R330F electrode has a response to BnOH with a linear range of 0.04 to 3.25 mM (R2=0.9985), a sensitivity of 122.78 μA mM−1 cm−2, and a detection limit of 0.03 mM (S/N=3). Coupling an electrochromic Prussian blue (PB) cathode helps the successful sensing visualization without any further power supply. The present sensing is more convenient and user‐friendly than the generally used gas chromatography (GC) and high performance liquid chromatography (HPLC), and brings a more accessible solution to the field of quality controlling.
Theoretical and Experimental Investigations of Stable Arylfluorene‐Based Radical‐Type Mechanophores
Authors: Sugita Hajime , Lu Yi , Aoki Daisuke , Otsuka Hideyuki , Mikami Koichiro 
Published: February 2023

AbstractInvited for the cover of this issue are the groups of Hideyuki Otsuka at the Tokyo Institute of Technology and Koichiro Mikami at the Sagami Chemical Research Institute. The image depicts theoretical and experimental investigations of stable arylfluorene‐based radical‐type mechanophores. Read the full text of the article at 10.1002/chem.202203249.
Photocatalytic Synthesis of Acetals and Ketals from Aldehydes and Silylenolethers without the Use of Acids
Authors: Steuernagel Desirée , Wagenknecht Hans‐Achim 
Published: February 2023

AbstractInvited for the cover of this issue are Desirée Steuernagel and Hans‐Achim Wagenknecht at the Karlsruhe Institute of Technology. The image depicts a traffic roundabout, which symbolizes the photocatalytic cycle for the conversion of aldehydes and silylenolethers as starting materials into their acetals. Read the full text of the article at 10.1002/chem.202203767.
Azatriseptanes: Strained Framework Analogs of [7,7,7]Circulenes
Authors: Zhang Kai , Hope Philip A. , El Bitar Nehme Mélissa , Linden Anthony , Spingler Bernhard , Rickhaus Michel 
Published: February 2023

AbstractInvited for the cover of this issue is the group of Michel Rickhaus at the University of Zurich. The image depicts the “unearthing” of the highly contorted azatriseptane, a carbon framework consisting of three fused seven‐membered rings surrounding a central nitrogen. Read the full text of the article at 10.1002/chem.202203954.
Preparation of molecularly imprinted silica particles with amino functionality for chiral separation of (±)‐naproxen
Authors: Bukhari Abeer Abdulaziz H. 
Published: February 2023

AbstractHerein is the synthesis and effective use of an S‐naproxen (S‐NP) enantioselective adsorbent material with amino functionality for the enantioseparation of a (±)‐NP racemic mixture. To begin, the S‐NP enantiomer of (3‐aminopropyl)triethoxysilane was used to create an amide derivative (S‐NP–Si–NH2). In order to incorporate the S‐NP enantiomeric species into the cross‐linked material, the developed S‐NP–Si–NH2 derivative was combined with tetraethoxysilane (TEOS) and subjected base‐catalyzed sol–gel condensation polymerization procedure. The S‐NP is released from the cross‐linked matrix by alkaline hydrolysis and subsequent acidification, creating an enantioselective gap filled with cationic ions that are compatible with the S‐NP during recombination. With the use of elemental analysis, nuclear magnetic resonance (NMR), and Fourier transform infrared (FTIR) spectroscopy, it was verified that the performed chemical processes on the monomeric precursor and the resulting S‐NP molecularly imprinted material (S‐NP–Sil) were successful. Furthermore, the morphology alterations were analyzed using scanning electron microscopy images of the sorbent surface. The produced adsorbent particles were also used in the chiral separation of a (±)‐NP racemic combination utilizing a column approach, with encouraging separation results shown in both the first loading run (59% ee of R‐NP) and the second elution run (85% ee of S‐NP).
Reading Time and DNA Sequence Preference of TET3 CXXC Domain Revealed by Single‐Molecule Profiling†
Authors: Wang Zeyu , Cao Zhiqiang , Ma Kangkang , Lu Man , Wang Meijie , Gao Han , Gong Deshun , Liang Lin , Yu Zhongbo 
Published: February 2023

Comprehensive SummaryRecognition of CpG dinucleotide DNA in epigenetic information flow plays a pivotal role for cellular differentiation and development. The TET3 CXXC domain binds to CpG DNA, serving a basic epigenetic information reading mechanism. During the selective recognition of a CpG motif by a CXXC domain from crowded binding sites in a gene sequence, the protein‐DNA interactions are beyond CpG dinucleotide. However, the selective binding dynamics of CpG within a long DNA context by epigenetic enzymes have been rarely exploited, which is hard for ensemble methods to probe. Here, we used single‐molecule magnetic tweezers to quantitatively examine the dynamics of TET3's CXXC domain on a Hoxa9 promoter DNA. Our single‐molecule binding profile revealed that CXXC‐DNA interactions involve both CpG motifs and their flanking sequences. The residence time of TET3 CXXC differs by about 1000 times in five distinguished CpG clusters in the context of a CpG island. Moreover, we performed multi‐state hidden Markov modeling analysis on the zipping/unzipping dynamics of a CpG hairpin, discovering TET3 CXXC's preference on CpG motifs regarding the –2 to +2 flanking bases. Our results shed light on the selective binding dynamics of a CXXC on a gene sequence, facilitating studies on epigenetic information reading mechanisms.
Imidazo[1,2‐a]pyridine Derivatives as AMPK Activators: Synthesis, Structure–Activity Relationships, and Regulation of Reactive Oxygen Species in Renal Fibroblasts
Authors: Wu Siming , Xiao Zhihong , Wei Junling , Zhang Lei , Cao Yuanyuan , Chen. Zhuo , Li Qianbin , Hu Gaoyun 
Published: February 2023

AbstractAdenosine 5′‐monophosphate activated protein kinase (AMPK) has emerged as a promising target for the discovery of drugs to treat diabetic nephropathy (DN). Herein, a series of imidazo[1,2‐a]pyridines were designed and synthesized. Among them, the active compound (EC50=11.0 nM) showed good enzyme activation and molecular docking results showed hydrogen bonding interactions with the key amino acids Asn111 and Lys29 in the active site. Meanwhile, further cellular level experiments revealed that it could reduce reactive oxygen species (ROS) levels in NRK‐49F cells induced by high glucose, and Western Blot experiments also demonstrate that it can increase the levels of p‐AMPK and p‐ACC and decrease the levels of TGF‐β1. The results of this study extend the structural types of AMPK activators and provide novel lead compounds for the subsequent development.
Design of OSMI‐4 Analogs Using Scaffold Hopping: Investigating the Importance of the Uridine Mimic in the Binding of OGT Inhibitors
Authors: Balsollier Cyril , Tomašič Tihomir , Yasini Daniel , Bijkerk Simon , Anderluh Marko , Pieters Roland J. 
Published: February 2023

Abstractβ‐N‐Acetylglucosamine transferase (OGT) inhibition is considered an important topic in medicinal chemistry. The involvement of O‐GlcNAcylation in several important biological pathways is pointing to OGT as a potential therapeutic target. The field of OGT inhibitors drastically changed after the discovery of the 7‐quinolone‐4‐carboxamide scaffold and its optimization to the first nanomolar OGT inhibitor: OSMI‐4. While OSMI‐4 is still the most potent inhibitor reported to date, its physicochemical properties are limiting its use as a potential drug candidate as well as a biological tool. In this study, we have introduced a simple modification (elongation) of the peptide part of OSMI‐4 that limits the unwanted cyclisation during OSMI‐4 synthesis while retaining OGT inhibitory potency. Secondly, we have kept this modified peptide unchanged while incorporating new sulfonamide UDP mimics to try to improve binding of newly designed OGT inhibitors in the UDP‐binding site. With the use of computational methods, a small library of OSMI‐4 derivatives was designed, prepared and evaluated that provided information about the OGT binding pocket and its specificity toward quinolone‐4‐carboxamides.
Palladium‐Catalyzed Carbonylation for General Synthesis of Aurones Using CO2
Authors: Huang Zijun , Li Yudong , Zhou Jiaxing , Zhang Yi , Wu Jiacheng , Wu Yongkai , Zhang Fan , Fang Zhengjun , Li Yuehui 
Published: February 2023

AbstractThe carbonylation of alkynes using CO2 to generate aurones is to date unknown. In this study, a palladium‐catalyzed carbonylation of terminal aromatic alkynes and the waste hydrosilane, poly(methylhydrosiloxane) (PMHS), is carried out with 2‐iodophenol using CO2 to produce aurones. A variety of terminal alkynes and substituted 2‐iodophenols are transformed into aurones in good yields. Preliminary mechanistic studies indicate that silyl formate, generated from CO2 and PMHS, plays a crucial role in the carbonylation reaction.
Third‐order quadrature sinusoidal oscillators with fully uncoupled tuning laws using only two CFOAs and grounded capacitors
Authors: Bhaskar Data Ram , Raj Ajishek , Senani Raj 
Published: February 2023

SummaryRecently, three current feedback operational amplifiers (CFOA)‐based third‐order quadrature sinusoidal oscillators (TOQSO) have been presented whose condition of oscillation (CO) and frequency of oscillation (FO) can be regulated independently. In this communication, we present two new TOQSO circuits, which require only two CFOAs and yet achieve as significant features as the quoted circuits, namely, the availability of fully uncoupled control of CO and FO, availability of both outputs from ideally zero output impedance nodes (which is beneficial for easy cascadability in VM operations) and employment of all (three) grounded capacitors as preferred for IC implementation. The verification of the workability of proposed circuits has been carried out experimentally using commercially available off‐the‐shelf ICs AD844‐type CFOAs, and some experimental results have been appended.
THADA‐IGF2BP3 fusions detected in fine‐needle aspiration specimens of thyroid nodules: An institutional experience
Authors: Gubbiotti Maria A. , Andrianus Stefen , Baloch Zubair 
Published: February 2023

AbstractIntroductionThough the clinical significance of THADA‐IGF2BP3 fusions detected in thyroid nodules preoperatively is still under investigation, the limited literature suggests these lesions are clinically low‐risk.MethodsThe pathology archives were searched from 2018 to 2022 for all thyroid nodules with a THADA‐IGF2BP3 fusion detected via ThyroSeqV3® analysis. Patient demographics and tumor characteristics were collected. Statistical analyses were performed and p < .05 was considered statistically significant. This study was approved by the institutional review board.ResultsThe case cohort included 34 thyroid nodules with THADA‐IGF2BP3 fusions from 32 patients (average age—56.1 years, range: 30–86, male to female ratio—10:22). The average nodule size was 3.2 cm (range: 1.3–7.3 cm). At the time of biopsy, 21 cases were diagnosed as atypia of undetermined significance/follicular lesion of undetermined significance, 12 as follicular neoplasm/suspicious for follicular neoplasm and 1 as suspicious for malignancy. Surgical resection was performed in 29 patients (13 partial and 16 total thyroidectomies) to give a total of 31 nodules. Papillary thyroid carcinoma was diagnosed in 19/31 (61%) cases. No cases showed extrathyroidal extension or lymphovascular invasion. The remaining cases were considered either a low‐risk neoplasm or benign. Four cases of NIFTP on final surgical pathology harbored concurrent incidental papillary thyroid microcarcinoma. One patient had the THADA‐IGF2BP3 fusion detected in bilateral nodules.ConclusionsTHADA‐IGF2BP3 fusions can occur in malignant, low‐risk and benign thyroid neoplasms, where malignant neoplasms show lack of aggressive features. Therefore, such entities can be classified as clinically low risk.
Climate‐related range shifts in Arctic‐breeding shorebirds
Authors: Anderson Christine M. , Fahrig Lenore , Rausch Jennie , Martin Jean‐Louis , Daufresne Tanguy , Smith Paul A. 
Published: February 2023

AbstractAimTo test whether the occupancy of shorebirds has changed in the eastern Canadian Arctic, and whether these changes could indicate that shorebird distributions are shifting in response to long‐term climate change.LocationFoxe Basin and Rasmussen Lowlands, Nunavut, Canada.MethodsWe used a unique set of observations, made 25 years apart, using general linear models to test if there was a relationship between changes in shorebird species' occupancy and their species temperature Index, a simple version of a species climate envelope.ResultsChanges in occupancy and density varied widely across species, with some increasing and some decreasing. This is despite that overall population trends are known to be negative for all of these species based on surveys during migration. The changes in occupancy that we observed were positively related to the species temperature index, such that the warmer‐breeding species appear to be moving into these regions, while colder‐breeding species appear to be shifting out of the regions, likely northward.Main ConclusionsOur results suggest that we should be concerned about declining breeding habitat availability for bird species whose current breeding ranges are centered on higher and colder latitudes.