bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tönnis angle and acetabular depth ratio: predictors of dislocation following hemiarthroplasty for hip fracture
Authors: Yassin Mohamed , Gupta Vatsal , Ramoutar Darryl , Hunter Thomas 
Published: December 2022

Background Despite efforts to mitigate possible modifiable risk factors, dislocations following hemiarthroplasty for hip fracture still occur, with reported incidence rates ranging from 1.5 to 11%. The aim of this study was to investigate whether acetabular dysplasia is a significant contributing factor to dislocation, and since non-modifiable, whether it should affect patient selection for this treatment option. Methods This is a multicentre nested case-control study of patients treated at 2 separate centres over a 10-year period from January 2011 to December 2020. All cases of hemiarthroplasty dislocation following hip fracture were identified from local databases, and 4 random controls were selected for every case. Tönnis angle (TA) and Acetabular-depth-ratio (ADR) was measured on the injured side using AP pelvis radiographs. Patients with TA > 10° and/or ADR < 250 were considered to have abnormal acetabular morphology. Results 35 cases of dislocation were identified and 140 random controls were selected. Cases of dislocation had a larger mean TA (8.8° vs. 5.5°, p < 0.001) and lower mean ADR (254 vs. 289, p < 0.001) than controls. 20 out of 35 (57%) cases were considered to have abnormal acetabular morphology, compared to 19 out of 140 (14%) controls. The odds of dislocation is 8.5 times greater (odds ratio = 8.49, 95%CI = 3.73, 19.39) in patients with abnormal TA and/or ADR. Conclusions This study demonstrates that TA > 10° and ADR < 250 are useful criteria to identify patients at greater risk of hip hemiarthroplasty dislocation due to acetabular dysplasia. Special consideration to preoperative planning should be taken in these patients. Future research should focus on methods to minimise risk in this subset of patients, including evaluating total hip arthroplasty with dual mobility component vs hemiarthroplasty.
An Analysis of Malpractice Litigation of Vascular Surgeons in Cases Involving Aortic Pathologies
Authors: Sen Indrani , Choudhry Asad , Cherukuri Sai Kiran , Mendes Bernardo C. , Colglazier Jill J. , Shuja Fahad , DeMartino Randall R. , Rasmussen Todd E. , Kalra Manju 
Published: December 2022

Objective The aim of this study was to analyze malpractice claims for aortic pathologies and to assess if there has been a change in rate of malpractice lawsuits with evolution of endovascular therapy. Methods Malpractice lawsuits were individually screened and compiled from the Westlaw database from 2000 to 2017 through use of relevant search terms. Data were collected of allegations, diagnoses, and outcomes of each case and compared. Results 268 unique cases were included in this study, with aneurysms (54%, n = 145) and dissection (35%, n = 94) making up the majority. There was a defendant verdict in 53% (n = 141), plaintiff verdict in 24% (n = 65), and settlements in 23% (n = 62) of lawsuits. Litigation was higher in the Midwest and Northeast. There was a gradual decline in litigation overall, however endovascular case numbers remained constant. There was negligible difference in the primary allegation underlying the litigation for various aortic pathologies, time to litigation and award between open and endovascular procedures. Conclusion The proportion of litigation for clinical negligence in endovascular cases amongst all vascular surgical lawsuits is increasing. As novel methods of endovascular therapy emerge, it is imperative that physicians remain vigilant to legal considerations to minimize malpractice risk.
Outcomes of ureteroscopy for management of stone disease in early and late childhood over a 15-year period
Authors: Sinha Mriganka , Pietropaolo Amelia , Quiroz Madarriaga Yesica , de Knecht Erika Llorens , Bujons Tur Anna , Griffin Stephen , Somani Bhaskar K 
Published: December 2022

Background: Although paediatric ureteroscopy is widely performed, there is still a lack of data and outcomes in early childhood. In this two-centre study, we compared the outcomes of ureteroscopy for stone disease management in early and late childhood and provide outcomes for the same. Methods: Data was retrospectively collected on consecutive patients from two tertiary paediatric endo-urology European centres over a 15-year period (2006–2021). Patients were split into two groups, namely, early childhood (age ⩽ 9 years) and late childhood (age 9 to ⩽16 years). Outcomes including stone-free rate (SFR) and complications were compared between these two groups. Results: A total of 148 patients underwent 184 procedures (1.2 procedure/patient) during the study period (66 in early childhood and 82 in late childhood). The mean age in early and late childhood groups were 5.6 and 13.3 years, and a male: female ratio of 1.6:1 and 1.1:1, respectively. The SFR and complications in early and late childhood groups were 87.8% and 90.2% ( p = 0.64) and 5.7% and 4.1%, respectively. Conclusion: Paediatric ureteroscopy and laser stone fragmentation achieves good results in both early and late childhood with comparable SFRs, although the complications and need for second procedure were marginally higher in the early childhood group. Our study would set up a new benchmark for patient counselling in future, and perhaps this needs to be reflected in the paediatric urolithiasis guidelines.
Diagnosis and treatment of 10 cases of idiopathic chondrolysis of the hip
Authors: Guan Tianan , Zhao Dun , Xiong Hao , Fang Bin , Li Yue 
Published: December 2022

Objective:To analyze the clinical characteristics, X-ray and magnetic resonance imaging manifestations, and treatment efficacy of idiopathic chondrolysis of the hip.Methods:Ten patients with idiopathic chondrolysis of the hip treated at our hospital from September 2013 to April 2022 were collected, and their clinical features, X-ray and magnetic resonance imaging manifestations, and treatment outcomes were analyzed.Results:Their main clinical features included single hip pain, claudication gait, and pelvic tilt, without specific clinical symptoms and signs. Laboratory tests such as blood analysis, erythrocyte sedimentation rate, C-reactive protein, rheumatoid factor, autoimmune test, coagulation function, interferon release test for tuberculosis infection, and tuberculosis antibody test were normal. In the late stage, the imaging shows degeneration or ankylosis of the joint. The disease progresses rapidly, and joint degeneration can occur in adolescence without effective treatment. Herein, seven patients were treated with recombinant human tumor necrosis factor receptor–antibody type II fusion protein. Among them, four (according to Camarnat magnetic resonance imaging classification, three were Stage I and one was Stage II) showed improved symptoms and function, while two (Stage III) had progressed. One patient (Stage I) who received recombinant human tumor necrosis factor receptor had no significant relief of symptoms, for whom anterior capsular release surgery significantly improved the hip joint motion. Of the four patients who underwent hip release surgery, three showed progression.Conclusion:Idiopathic chondrolysis of the hip has no specific clinical and laboratory tests and a high misdiagnosis rate. Thus, early magnetic resonance imaging is a critical reference for diagnosis. We recommend that patients with magnetic resonance imaging Stage II or earlier be actively treated with recombinant human tumor necrosis factor receptor and start treatment as early as possible. In the third stage, the treatment effect is mediocre, and the narrowed hip space is difficult to change.
Sliding Oblique Metatarsal Osteotomy Fixated With K-Wires Without Cheilectomy for All Grades of Hallux Rigidus: A Case Series of 76 Patients
Authors: Nakajima Kenichiro 
Published: December 2022

Background: Studies on decompression metatarsal osteotomy without cheilectomy for hallux rigidus are limited. This study aimed to review the data of patients who underwent this surgery for all grades of hallux rigidus. Methods: The medical records of patients who underwent this surgery between August 2017 and January 2021 were retrospectively reviewed. The outcomes were assessed using the visual analog scale (VAS), the Japanese Society for Surgery of the Foot (JSSF) score, and the hallux dorsiflexion angle. Patients who were followed up for more than 2 years were included. The plantar shift of the first metatarsal head (PS) and the shortening of the first metatarsal (SH) were measured to supplementally investigate the associations with other measurements. Results: A total of 76 patients (female, 42; male, 34; mean age, 60.3 years; mean body mass index, 23.1) with 80 feet were enrolled. Six patients (7.5%) were grade 1, 20 (25.0%) were grade 2, 30 (37.5%) were grade 3, and 24 (30.0%) were grade 4. The mean follow-up duration was 3.5 years. Overall, the mean VAS score improved from 63.2 to 6.2, the mean JSSF score from 59.0 to 82.6, and the mean dorsiflexion angle from 37.3 to 56.3 degrees. These measures improved for all grades, but the dorsiflexion angles and JSSF scores decreased as the grade advanced. No clear associations were found between the postoperative VAS and the PS and SH. Eight patients (10.5%) had complications: 4 (5.2%) had occasional plantar discomfort under the metatarsal head, 2 (2.6%) required shoe modification, 1 (1.3%) had wound pain, and 1 (1.3%) experienced breakage of a K-wire. Conclusion: The VAS scores, dorsiflexion angles, and JSSF scores improved for all grades of hallux rigidus within a relatively short period of follow-up; however, the dorsiflexion angles and JSSF scores decreased as the grade advanced. Level of Evidence: Level IV, retrospective case series.
Life cycle assessment of MycoWorks’ Reishi™: the first low-carbon and biodegradable alternative leather
Authors: Williams Ellie , Cenian Katarzyna , Golsteijn Laura , Morris Bill , Scullin Matthew L. 
Published: December 2022

Abstract
Background
Over the past few years, several alternative leather technologies have emerged and promise advantages over incumbent leathers with respect to sustainability despite most containing enough plastic to prevent safe and effective biodegradation. Of the alternative leathers in production or advanced development, few fit the dual criteria of low-carbon and near-zero plastic. Reishi™ is a leather alternative, grown using MycoWorks’ Fine Mycelium™ technology, with less than 1% polymer content and satisfies the same performance, quality, and hand feel as animal leather. We present here the first Life Cycle Assessment (LCA) of Reishi™, detailing its “cradle-to-gate” carbon footprint and broader environmental profile. The pilot- and full-scale production of 1 m2 of post-processed, finished, and packaged Reishi™ both before and after production improvement implementations is modeled, and the environmental footprint impact assessment method is performed.

Results
It was found that Reishi’s™ carbon footprint is as low as 2.76 kg CO2-eq per m2, or 8% of the value of the bovine leather benchmark modeled. Furthermore, it was found that Reishi™ has a lower impact compared to bovine leather modeled across a number of impact categories, including eutrophication, ecotoxicity, human health effects, and others. Reishi’s™ impact “hotspots” were determined, with the largest opportunity for further reduction being improved energy efficiency in the growth of mycelium, in particular, the process’s sterilization of raw material inputs via autoclave tools. It is also shown that MycoWorks’ passive process for growing mycelium has a carbon footprint two orders of magnitude lower than incumbent mycelium growth processes that actively consume carbon dioxide gas, which MycoWorks’ process does not require.

Conclusions
Reishi™ is shown to be a promising sustainable material through its unique combination of natural quality, low-carbon footprint as determined by this LCA, and biodegradability due to its lack of plastic or crosslinked content. Its manufacturing process is low impact even when produced at a scale of tens of thousands of square meters per year—a miniscule fraction of the billions of square meters of bovine leather already sold per year. With further use of this leather alternative, additional efficiency gains are likely to be realized.
A role for worm cutl-24 in background- and parent-of-origin-dependent ER stress resistance
Authors: Wang Wenke , Flury Anna G. , Rodriguez Andrew T. , Garrison Jennifer L. , Brem Rachel B. 
Published: December 2022

Abstract
Background
Organisms in the wild can acquire disease- and stress-resistance traits that outstrip the programs endogenous to humans. Finding the molecular basis of such natural resistance characters is a key goal of evolutionary genetics. Standard statistical-genetic methods toward this end can perform poorly in organismal systems that lack high rates of meiotic recombination, like Caenorhabditis worms.

Results
Here we discovered unique ER stress resistance in a wild Kenyan C. elegans isolate, which in inter-strain crosses was passed by hermaphrodite mothers to hybrid offspring. We developed an unbiased version of the reciprocal hemizygosity test, RH-seq, to explore the genetics of this parent-of-origin-dependent phenotype. Among top-scoring gene candidates from a partial-coverage RH-seq screen, we focused on the neuronally-expressed, cuticlin-like gene cutl-24 for validation. In gene-disruption and controlled crossing experiments, we found that cutl-24 was required in Kenyan hermaphrodite mothers for ER stress tolerance in their inter-strain hybrid offspring; cutl-24 was also a contributor to the trait in purebred backgrounds.

Conclusions
These data establish the Kenyan strain allele of cutl-24 as a determinant of a natural stress-resistant state, and they set a precedent for the dissection of natural trait diversity in invertebrate animals without the need for a panel of meiotic recombinants.
Bacterial dynamics during the burial of starch-based bioplastic and oxo-low-density-polyethylene in compost soil
Authors: Wicaksono Joshua Abednego , Purwadaria Tresnawati , Yulandi Adi , Tan Watumesa Agustina 
Published: December 2022

Abstract
Background
Plastic waste accumulation is one of the main ecological concerns in the past decades. A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastics and oxo-biodegradable plastics, is perceived as a solution to this issue. However, the fate of these materials in the environment are unclear, and less is known about how their presence affect the microorganisms that may play a role in their biodegradation. In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial carrier bags claimed as biodegradable: cassava starch-based bioplastic and oxo-low-density polyethylene (oxo-LDPE). Each type of plastic bag was buried separately in compost soil and incubated for 30, 60, 90, and 120 days. Following incubation, soil pH and temperature as well as the weight of remaining plastics were measured. Bacterial diversity in soil attached to the surface of remaining plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrRNA gene.

Results
After 120 days, the starch-based bioplastic weight has decreased by 74%, while the oxo-LDPE remained intact with only 3% weight reduction. The bacterial composition in soil fluctuated over time with or without the introduction of either type of plastic. While major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure. None of these bacteria were abundant continuously, but rather they emerged at specific time points. The introduction of plastics into soil increased not only the population of bacteria known for their ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders. Bacterial groups that are involved in nitrogen cycling also arose throughout burial.

Conclusions
The introduction of starch-based bioplastic and oxo-LDPE led to contrasting shift in soil bacterial population overtime, which may determine their fate in the environment.
Working towards arabinogalactan proteins (AGPs) from fruit: carbohydrate composition and impact on fungal growth
Authors: Leszczuk Agata , Zając Adrian , Cybulska Justyna , Stefaniuk Dawid , Zdunek Artur 
Published: December 2022

Abstract
Background
Arabinogalactan proteins (AGPs) are extracellular matrix constituents involved in plant response to fungal infection. The aim of the current study was to investigate the antifungal effect of AGPs ex situ and to determine the structural features of AGPs that may have an influence on this activity. The features of AGPs isolated from fruit were investigated with molecular tools based on specific monoclonal antibodies recognizing carbohydrate AGP epitopes. The Antifungal (well-diffusion) Susceptibility Test and the Agar Invasion Test were used to assess the impact of AGPs on Penicillium notatum culture.

Results
The results definitely ruled out the influence of AGPs on fungal growth. The immunochemical analyses revealed that AGPs consist mainly of carbohydrate chains composed of β-linked glucuronosyl residues recognized by LM2 and GlcA-β(1 → 3)-GalA-α(1 → 2) Rha recognized by JIM13, which do not have the same functional properties outside the plant cell in in vitro experimental conditions.

Conclusions
The action of a single cell wall component does not elicit any influence ex situ. The extensive accumulation of glycan chains of AGPs in infected tissue as a result of a complex mechanism occurring in the cell wall emphasizes the importance of dependencies between particular components of the extracellular matrix in response to fungal attack.
The REACT study: design of a randomized phase 3 trial to assess the efficacy and safety of clazosentan for preventing deterioration due to delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage
Authors: Bruder Nicolas , Higashida Randall , Santin-Janin Hugues , Dubois Cécile , Aldrich E. François , Marr Angelina , Roux Sébastien , Mayer Stephan A. 
Published: December 2022

Abstract
Background
For patients presenting with an aneurysmal subarachnoid hemorrhage (aSAH), delayed cerebral ischemia (DCI) is a significant cause of morbidity and mortality. The REACT study is designed to assess the safety and efficacy of clazosentan in preventing clinical deterioration due to DCI in patients with aSAH.

Methods
REACT is a prospective, multicenter, randomized phase 3 study that is planned to enroll 400 patients with documented aSAH from a ruptured cerebral aneurysm, randomized 1:1 to 15 mg/hour intravenous clazosentan vs. placebo, in approximately 100 sites and 15 countries. Eligible patients are required to present at hospital admission with CT evidence of significant subarachnoid blood, defined as a thick and diffuse clot that is more than 4 mm in thickness and involves 3 or more basal cisterns. The primary efficacy endpoint is the occurrence of clinical deterioration due to DCI up to 14 days post-study drug initiation. The main secondary endpoint is the occurrence of clinically relevant cerebral infarction at Day 16 post-study drug initiation. Other secondary endpoints include the modified Rankin Scale (mRS) and the Glasgow Outcome Scale-Extended (GOSE) score at Week 12 post-aSAH, dichotomized into poor and good outcome. Radiological results and clinical endpoints are centrally evaluated by independent committees, blinded to treatment allocation. Exploratory efficacy endpoints comprise the assessment of cognition status at 12 weeks and quality of life at 12 and 24 weeks post aSAH.

Discussion
In the REACT study, clazosentan is evaluated on top of standard of care to determine if it reduces the risk of clinical deterioration due to DCI after aSAH. The selection of patients with thick and diffuse clots is intended to assess the benefit/risk profile of clazosentan in a population at high risk of vasospasm-related ischemic complications post-aSAH.

Trial registration (Additional file 1)
ClinicalTrials.gov (NCT03585270). EU Clinical Trial Register (EudraCT Number: 2018–000241-39).
Interactions of the chemokines CXCL11 and CXCL12 in human tumor cells
Authors: Koch Christian , Fischer Nina Charlotte , Puchert Malte , Engele Jürgen 
Published: December 2022

Abstract
Background
The chemokines, CXCL12 and CXCL11, are upregulated in tumors from many organs and control their progression. CXCL12 and CXCL11 affect tumor cell functions by either binding their prime receptors, CXCR4 and CXCR3, respectively, and/or CXCR7 as a common second chemokine receptor. In humans, CXCR3 exists in the functional splice variants, CXCR3A and CXCR3B, which either have pro- or anti-tumor activity, respectively. Despite the intimate crosstalk between the CXCL12- and CXCL11-system, the impact of a combination of CXCL12 and CXCL11 on tumor progression remains vague.

Methods
In the present work, we have analyzed CXCL12 and CXCL11 for combined effects on migration, invasion, proliferation, and cytostatic-induced apoptosis of the human tumor cells, A549, A767, A772, DLD-1, and MDA-MB-231.

Results
We demonstrate that the mode of interaction differs with respect to cell type and function and allows for either potentiation, attenuation or no changes of cellular responses. The divergent responses are not the result of the distinct use of different CXCL12- and CXCL11-receptors by the respective tumor cells, but in case of cell migration seem to be associated with the activation of p38 signaling pathways.

Conclusions
Our findings point to therapeutic limitations of ongoing efforts to selectively target CXCR3, CXCR4, or CXCR7 in cancer patients, and rather favor individualized targeting strategies.
Assessment of outer retinal thickness and function in mice after experimental optic nerve trauma
Authors: Lypka Karin Rose , Carmy-Bennun Tal , Garces Kimberly N. , Venanzi Alexander W. , Hackam Abigail S. 
Published: December 2022

Abstract
Background
Optic nerve trauma caused by crush injury is frequently used for investigating experimental treatments that protect retinal ganglion cells (RGCs) and induce axonal regrowth. Retaining outer retinal light responses is essential for therapeutic rescue of RGCs after injury. However, whether optic nerve crush also damages the structure or function of photoreceptors has not been systematically investigated. In this study, we investigated whether outer retinal thickness and visual function are altered by optic nerve crush in the mouse.

Methods
Wildtype mice underwent optic nerve crush and intravitreal injection of a control solution in one eye with the fellow eye remaining uninjured. Two weeks after injury, the thickness of the ganglion cell region (GCL to IPL) and photoreceptor layer (bottom of the OPL to top of the RPE) were measured using OCT. Retinal function was assessed using flash ERGs. Immunodetection of RGCs was performed on retinal cryosections and RGCs and ONL nuclei rows were counted. Multiple comparison analyses were conducted using Analysis of Variance (ANOVA) with Tukey’s post hoc test and P values less than 0.05 were considered statistically significant.

Results
Optic nerve crush injury induced RGC death as expected, demonstrated by thinning of the ganglion cell region and RGC loss. In contrast, outer retinal thickness, photopic and scotopic a-wave and b-wave amplitudes and photoreceptor nuclei counts, were equivalent between injured and uninjured eyes.

Conclusions
Secondary degeneration of the outer retina was not detected after optic nerve injury in the presence of significant RGC death, suggesting that the retina has the capacity to compartmentalize damage. These findings also indicate that experimental treatments to preserve the GCL and rescue vision using this optic nerve injury model would not require additional strategies to preserve the ONL.