bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virtual Reality Application for Vestibular/Ocular Motor Screening: Current Clinical Protocol Versus a Novel Prototype
Authors: Pavilionis Philip , Adhanom Isayas Berhe , Moran Ryan , Taylor Madison R. , Murray Nicholas G. 
Published: March 2023

Background: Virtual reality (VR) has been explored to improve baseline and postinjury assessments in sport-related concussion (SRC). Some experience symptoms related to VR, unrelated to concussion. This may deter use of vestibular/ocular motor screening (VOMS) using VR. Baseline VR VOMS symptomatology differentiates baseline from overall symptomatology. Hypothesis: There will be no difference between current clinical manual VOMS (MAN), a clinical prototype (PRO), and VR for symptom provocation change score (SPCS) and near point of convergence (NPC) average score in a healthy population and sex differences among the 3 modes of administration. Study Design: Cohort study. Level of Evidence: Level 3. Methods: A total of 688 National Collegiate Athletic Association Division I student-athletes completed VOMS using 3 methods (MAN, N = 111; female athletes, N = 47; male athletes, N = 64; average age, 21 years; PRO, N = 365; female athletes, N = 154; male athletes, N = 211; average age, 21 years; VR, N = 212; female athletes, N = 78; male athletes, N = 134; average age = 20 years) over a 3-year period (2019-2021) during annual baseline testing. Exclusion criteria were as follows: self-reported motion sickness in the past 6 months, existing or previous neurological insult, attention deficit hyperactivity disorder, learning disabilities, or noncorrected vision impairment. Administration of MAN followed the current clinical protocols, PRO used a novel prototype, and VR used an HTC Vive Pro Eye head mounted display. Symptom provocation was compared using Mann-Whitney U tests across each VOMS subtest with total SPCS and NPC average by each method. Results: MAN had significantly ( P < 0.01) more baseline SPCS (MAN = 0.466 ± 1.165, PRO = 0.163 ± 0.644, VR = 0.161 ± 0.933) and significantly ( P < 0.01) and more SPCS (MAN = 0.396 ± 1.081, PRO = 0.128 ± 0.427, VR = 0.48 ± 0.845) when compared with PRO and VR. NPC average measurements for VR (average, 2.99 ± 0.684 cm) were significantly greater than MAN (average, 2.91 ± 3.35 cm; P < 0.01; Cohen’s d = 0.03) and PRO (average, 2.21 ± 1.81 cm; P < 0.01; Cohen’s d = 0.57). For sex differences, female athletes reported greater SPCS with PRO (female athletes, 0.29 ± 0.87; male athletes, 0.06 ± 0.29; P < 0.01) but not in VR or MAN. Conclusion: Using a VR system to administer the VOMS may not elicit additional symptoms, resulting in fewer false positives and is somewhat stable between sexes. Clinical Relevance: VOMS may allow for standardization among administrators and reduce possible false positives.
Successful rescue treatment using chlorambucil for presumed recurrence of sinonasal lymphoma in a cat
Authors: Goto Sho , Muto Fumikazu , Iwasaki Ryota , Mori Takashi 
Published: March 2023

Case summary A 12-year-old male neutered domestic shorthair cat presented with a 2-week history of serous unilateral nasal discharge, swelling of the nasal bridge and sneezing. Whole-body CT revealed a mass filling the entire right nasal cavity with lysis of the cribriform plate. The cat was diagnosed with sinonasal large-cell lymphoma based on cytopathological analysis, with PCR-based lymphocyte clonality testing showing a monoclonal population with rearrangement of the immunoglobulin heavy chain gene. The cat received radiotherapy with a dose of 30 Gy in seven fractions given three times weekly, and then cyclophosphamide, doxorubicin, vincristine and prednisolone (CHOP)-based chemotherapy was initiated soon after completion of the radiotherapy schedule. Despite this treatment, CT performed 4 months after radiotherapy revealed enlargement of the lesion in the right nasal cavity consistent with presumed progression of the cat’s lymphoma. The cat then received rescue chemotherapy with chlorambucil, which markedly reduced the size of the disease burden in the nasal and frontal sinus without severe adverse effects. At the time of writing, the cat was receiving chlorambucil for 7 months without any clinical signs suggestive of tumour relapse. Relevance and novel information To our knowledge, this is the first case of feline sinonasal lymphoma with chlorambucil used as rescue chemotherapy. This case indicates that chemotherapy with chlorambucil may be a useful treatment option for cats with relapsing sinonasal lymphoma following radiotherapy and/or CHOP-based chemotherapy.
Work Challenges and Health of Immigrant Hotel Housekeepers: Part 2
Authors: Hsieh Yu-Chin (Jerrie) , Shapoval Valeriya , Sönmez Sevil , Apostolopoulos Yorghos 
Published: March 2023

Background: Hotel housekeepers’ major responsibility is to perform cleaning tasks. In the course of performing their duties, hotel housekeepers are disproportionately exposed to multiple workplace hazards (i.e., physical, chemical, biological, and psychosocial hazards) that put them at risk of adverse health consequences. This study aims to discover the occupational and health challenges encountered by immigrant hotel housekeepers in Orlando, Florida, and help hotel management to develop strategies to improve their occupational health. Methods: This study adopted an interviewer-administered survey method for data collection. Data were collected from members of the United Here Union, Local 7373 in Orlando, Florida. Trained Spanish–English bilingual associates of the Union administered the surveys through interviews with participants. Descriptive statistics and stepwise multiple regression were employed to analyze data ( n = 140) using SPSS 22. Findings: This study shows that Florida hotel housekeepers participating in this study worked under great time pressures, endured excessive workloads, did not have enough time to rest and recover, and often skipped or shortened lunch breaks. Participants believed that their employers valued work productivity more than their safety and health. More than half of the respondents experienced racial discrimination at their workplaces. Conclusion/Application to Practice: The article brings an important contribution to the awareness of housekeepers’ feelings toward their work and the need for effective safety and health policies and programs.
Work-Related Factors Associated With the Pelvic Floor Dysfunction Among a Sample of Female Nurses in China
Authors: Rumeng Chen , Ya Zhu , Xirong Chen , Yue Su , Lixia Chen , Shenshen Wang , Chao Jin , Chunxia Zhang 
Published: March 2023

Background: Female nurses are a high-risk group for pelvic floor dysfunction (PFD). Predictors of female nurses’ PFD among work-related factors are not well known. The aim of this study was to investigate the prevalence of PFD and its association with workplace conditions among female nurses in China. Methods: An online cross-sectional survey was conducted in May 2021. A sample of 380 registered nurses working in six tertiary hospitals in Nanjing, China participated. Data on individual characteristics, work-related factors, the Pelvic Floor Distress Inventory-20 and Pelvic Floor Impact Questionnaire-7 were used. Findings: The overall prevalence of PFD among nurses was 83.9%, with 43.9% of participants experiencing pelvic organ prolapse, 66.6% experiencing anorectal dysfunction and 60.5% experiencing lower urinary tract symptoms. In terms of work-related factors, PFD was associated with heavy lifting, pushing, and carrying at work, delayed toileting at work and fluid intake. Female nurses with PFD reported lower quality of life (QoL) relative to nurses without PFD. Conclusions/Application to Practice: Our findings provide initial insights into workplace conditions that promote PFD among female nurses. Occupational health nurses should consider providing educational information for female workers who are potentially at risk for PFD and consider integrating screening of PFD into practice.
Paper 10: Return-to-Play Following Latarjet Procedure in Young Contact Athletes
Authors: Sridharan Mathangi , Patel Akshar , Swinehart Steven , Rauck Ryan , Cvetanovich Gregory , Bishop Julie 
Published: March 2023

Objectives: Young athletes that play a contact sport are susceptible to anterior glenohumeral instability due to high-velocity impacts and repetitive dislocations. Few studies have examined return-to play and patient outcomes following Latarjet procedure in this patient population. The objective of this study was to investigate postoperative return to contact sports in athletes undergoing the Latarjet procedure for shoulder instability. Methods: 67 consecutive contact athletes (71 shoulders), age ≤35, that underwent Latarjet procedure for recurrent shoulder instability between 1/1/2018 and 3/31/2021 were retrospectively identified. Demographic information, medical history, surgical history, number of dislocations prior to surgery, and post-operative complications up to 6 months after surgery were reviewed. 42 patients were interviewed with minimum follow-up of 12 months. Outcomes evaluated include return to play, competition level, patient satisfaction, and patient-reported outcomes including American Shoulder and Elbow Surgeons (ASES) Score, Disabilities of the Arm, Shoulder, and Hand (DASH) Score, and Visual Analog Scale (VAS). Results: Mean age at time of surgery for the total cohort was 19.7 years. 19/67 (28.3%) of patients had prior shoulder surgery, most commonly arthroscopic stabilization. On average, patients had 24.2 frank dislocations (SD 39.1) prior to undergoing Latarjet procedure. 8/67 (11.9%) of patients experienced unresolved pain or stiffness six months after surgery and 2/67 (2.9%) required re-operation after Latarjet. Only one patient experienced hardware failure. 42 patients (63%) were interviewed with mean follow-up of 26.7 months, of which 20 played football. 14/42 (33%) athletes competed at the collegiate level, and 23/42 (55%) at the high school level at the time of surgery. 33/42 patients (78.6%) returned to sport, of which 29/33 (88%) returned to playing at the same competition level; 12/20 (60%) of football players returned to sport. 3/42 (7%) reported recurrent dislocation. Mean ASES score was 91.7, DASH score 6.1, and visual analog scale 4. 38/42 (90%) patients reported improvement in quality of life after undergoing Latarjet procedure for shoulder instability. Conclusions: The Latarjet procedure allows young contact athletes with shoulder instability to return to competitive play at strong rates. Although there is high patient satisfaction with the Latarjet procedure, recurrent instability and unresolved pain and stiffness are significant post-operative complications.
The effectiveness of penile curvature treatment by cavernous body rotation and plication of the tunica albuginea
Authors: Vorobev Vladimir , Beloborodov Vladimir , Sherbatykh Andrey , Kalyagin Alexey , Baklanova Olga , Popov Sergei , Sidorov Stepan 
Published: March 2023

Abstract
Background
There are several approaches to the surgical treatment of the penile curvature conditionally divided into three large groups: tunica albuginea plication (TAP), corpus cavernosum rotation (CR), and transplantation of various materials. The study aims to compare the effectiveness of TAP and CR techniques in the treatment of penile curvature. There was a prospective randomized study of the effectiveness of surgical treatment of patients with an established diagnosis of the penile curvature from 2017 to 2020 in Irkutsk, Russian Federation. The final analysis of the results included 22 cases.

Results
The analysis of the comparative intergroup effectiveness of the treatment performed based on the criteria established in the study showed good treatment results in 8 (88.8%) patients in the CR group and 9 (69.2%) patients in the TAP group (p = 0.577). The other patients obtained satisfactory results. There were no negative outcomes. Simple logistic regression analysis showed that the preoperative flexion angle > 60 degrees (OR 2.7; 95% CI 0.12; 5.28; p = 0.040) was significant in predicting the complaints of penile shortening during TAP. Both methods are safe, effective, and bring minimal risk of complications.

Conclusion
Thus, the effectiveness of both treatment methods is comparable. However, TAP surgery is not recommended for patients with an initial curvature of more than 60 degrees.
CellTrackVis: interactive browser-based visualization for analyzing cell trajectories and lineages
Authors: Shim Changbeom , Kim Wooil , Nguyen Tran Thien Dat , Kim Du Yong , Choi Yu Suk , Chung Yon Dohn 
Published: March 2023

Abstract
Background
Automatic cell tracking methods enable practitioners to analyze cell behaviors efficiently. Notwithstanding the continuous development of relevant software, user-friendly visualization tools have room for further improvements. Typical visualization mostly comes with main cell tracking tools as a simple plug-in, or relies on specific software/platforms. Although some tools are standalone, limited visual interactivity is provided, or otherwise cell tracking outputs are partially visualized.

Results
This paper proposes a self-reliant visualization system, CellTrackVis, to support quick and easy analysis of cell behaviors. Interconnected views help users discover meaningful patterns of cell motions and divisions in common web browsers. Specifically, cell trajectory, lineage, and quantified information are respectively visualized in a coordinated interface. In particular, immediate interactions among modules enable the study of cell tracking outputs to be more effective, and also each component is highly customizable for various biological tasks.

Conclusions
CellTrackVis is a standalone browser-based visualization tool. Source codes and data sets are freely available at http://github.com/scbeom/celltrackvis with the tutorial at http://scbeom.github.io/ctv_tutorial.
Analysis of the AMT gene family in chili pepper and the effects of arbuscular mycorrhizal colonization on the expression patterns of CaAMT2 genes
Authors: Fang Lei , Wang Miaomiao , Chen Xiao , Zhao Jianrong , Wang Jianfei , Liu Jianjian 
Published: March 2023

Abstract
Background
Ammonium (NH4+) is a key nitrogen source supporting plant growth and development. Proteins in the ammonium transporter (AMT) family mediate the movement of NH4+ across the cell membrane. Although several studies have examined AMT genes in various plant species, few studies of the AMT gene family have been conducted in chili pepper.

Results
Here, a total of eight AMT genes were identified in chili pepper, and their exon/intron structures, phylogenetic relationships, and expression patterns in response to arbuscular mycorrhizal (AM) colonization were explored. Synteny analyses among chili pepper, tomato, eggplant, soybean, and Medicago revealed that the CaAMT2;1, CaAMT2.4, and CaAMT3;1 have undergone an expansion prior to the divergence of Solanaceae and Leguminosae. The expression of six AMT2 genes was either up-regulated or down-regulated in response to AM colonization. The expression of CaAMT2;1/2;2/2;3 and SlAMT2;1/2;2/2;3 was significantly up-regulated in AM fungi-inoculated roots. A 1,112-bp CaAMT2;1 promoter fragment and a 1,400-bp CaAMT2;2 promoter fragment drove the expression of the β-glucuronidase gene in the cortex of AM roots. Evaluation of AM colonization under different NH4+ concentrations revealed that a sufficient, but not excessive, supply of NH4+ promotes the growth of chili pepper and the colonization of AM. Furthermore, we demonstrated that CaAMT2;2 overexpression could mediate NH4+ uptake in tomato plants.

Conclusion
In sum, our results provide new insights into the evolutionary relationships and functional divergence of chili pepper AMT genes. We also identified putative AMT genes expressed in AM symbiotic roots.
Gene enrichment and co-expression analysis shed light on transcriptional responses to Ralstonia solanacearum in tomato
Authors: Shi Jianlei , Shui Deju , Su Shiwen , Xiong Zili , Zai Wenshan 
Published: March 2023

Abstract
Background
Tomato (Solanum lycopersicum) is both an important agricultural product and an excellent model system for studying plant-pathogen interactions. It is susceptible to bacterial wilt caused by Ralstonia solanacearum (Rs), and infection can result in severe yield and quality losses. To investigate which genes are involved in the resistance response to this pathogen, we sequenced the transcriptomes of both resistant and susceptible tomato inbred lines before and after Rs inoculation.

Results
In total, 75.02 Gb of high-quality reads were generated from 12 RNA-seq libraries. A total of 1,312 differentially expressed genes (DEGs) were identified, including 693 up-regulated and 621 down-regulated genes. Additionally, 836 unique DEGs were obtained when comparing two tomato lines, including 27 co-expression hub genes. A total of 1,290 DEGs were functionally annotated using eight databases, most of which were found to be involved in biological pathways such as DNA and chromatin activity, plant-pathogen interaction, plant hormone signal transduction, secondary metabolite biosynthesis, and defense response. Among the core-enriched genes in 12 key pathways related to resistance, 36 genotype-specific DEGs were identified. RT-qPCR integrated analysis revealed that multiple DEGs may play a significant role in tomato response to Rs. In particular, Solyc01g073985.1 (NLR disease resistance protein) and Solyc04g058170.1 (calcium-binding protein) in plant-pathogen interaction are likely to be involved in the resistance.

Conclusion
We analyzed the transcriptomes of both resistant and susceptible tomato lines during control and inoculated conditions and identified several key genotype-specific hub genes involved in a variety of different biological processes. These findings lay a foundation for better understanding the molecular basis by which resistant tomato lines respond to Rs.
A multi-disciplinary program for opioid sparse arthroplasty results in reduced long-term opioid consumption: a four year prospective study
Authors: Lin D-Yin , Samson Anthony J. , D’Mello Freeda , Brown Brigid , Cehic Matthew G. , Wilson Christopher , Kroon Hidde M. , Jaarsma Ruurd L. 
Published: March 2023

Abstract
Introduction
The current opioid epidemic poses patient safety and economic burdens to healthcare systems worldwide. Postoperative prescriptions of opioids contribute, with reported opioid prescription rates following arthroplasty as high as 89%. In this multi-centre prospective study, an opioid sparing protocol was implemented for patients undergoing knee or hip arthroplasty. The primary outcome is to report our patient outcomes in the context of this protocol, and to examine the rate of opioid prescription on discharge from our hospitals following joint arthroplasty surgery. This is possibly associated with the efficacy of the newly implemented Arthroplasty Patient Care Protocol.

Methods
Over three years, patients underwent perioperative education with the expectation to be opioid-free after surgery. Intraoperative regional analgesia, early postoperative mobilisation and multimodal analgesia were mandatory. Long-term opioid medication use was monitored and PROMs (Oxford Knee/Hip Score (OKS/OHS), EQ-5D-5 L) were evaluated pre-operatively, and at 6 weeks, 6 months and 1 year postoperatively. Primary and secondary outcomes were opiate use and PROMs at different time points.

Results
A total of 1,444 patients participated. Two (0.2%) knee patients used opioids to one year. Zero hip patients used opioids postoperatively at any time point after six weeks (p < 0.0001). The OKS and EQ-5D-5 L both improved for knee patients from 16 (12–22) pre-operatively to 35 (27–43) at 1 year postoperatively, and 70 (60–80) preoperatively to 80 (70–90) at 1 year postoperatively (p < 0.0001). The OHS and EQ-5D-5 L both improved for hip patients from 12 (8–19) preoperatively to 44 (36–47) at 1 year postoperatively, and 65 (50–75) preoperatively to 85 (75–90) at 1 year postoperatively (p < 0.0001). Satisfaction improved between all pre- and postoperative time points for both knee and hip patients (p < 0.0001).

Conclusions
Knee and hip arthroplasty patients receiving a peri-operative education program can effectively and satisfactorily be managed without long-term opioids when coupled with multimodal perioperative management, making this a valuable approach to reduce chronic opioid use.
A rare focal atrial tachycardia arising from the proximal middle cardiac vein: a case report
Authors: Guo Min , Zhang Nan , Jia Gao , Ma Guijin , Li Xin , Wang Rui 
Published: March 2023

Abstract
Background
Focal atrial tachycardia (FAT) always originates from atrium specific sites and can be successfully cured by radiofrequency (RF) ablation. However, the middle cardiac vein (MCV) is a rare site of focal atrial tachycardia. Herein, we present a case of a 20-year-old young woman with FAT. Electrophysiological examination showed FAT arising from the proximal middle cardiac vein (pMCV), and successful RF ablation was applied with a low power and short-ablation.

Case presentation
A 20-year-old woman with no structural heart disease suffered recurrent supraventricular tachycardia for 1 year. Physical examination, laboratory studies and the echocardiography results of this patient were normal. A 12-lead electrocardiogram (ECG) showed a narrow QRS and long RP tachycardia which was always triggered by a sinus rhythm. The patient underwent an electrophysiological study and found the earliest activation was in the proximal MCV (pMCV). After a low power and short-ablation, AT was terminated and noninducible by programmed pacing with or without isoproterenol infusion.

Conclusion
This case presented a rare case of FAT arising from the pMCV. We demonstrate that low power and short-ablation are effective in AT arising from specific areas such as the coronary sinus ostium and pMCV.
Relationship between income and concerns about physical changes and help-seeking by older adult cancer survivors: a secondary analysis
Authors: Nicoll Irene , Lockwood Gina , Strohschein Fay J. , Fitch Lauren , Longo Christopher J. , Newton Lorelei , Fitch Margaret I. 
Published: March 2023

Abstract
Objective
Globally, the number of older adults surviving cancer is anticipated to grow rapidly over the next decades. Cancer and its treatment can leave survivors with a myriad of challenges including physical changes which impact independence and quality of life. This project explored the relationship of income level with concerns and help-seeking for physical changes following treatment in older Canadian survivors of cancer.

Methods
A Canada-wide survey of community-dwelling survivors of cancer explored their experiences with survivorship care one to three years following completion of treatment. A secondary trend analysis examined the relationship of income with older adults’ level of concern and help-seeking experiences regarding physical consequences they attributed to their cancer treatment.

Results
In total, 7,975 people aged 65 years and older who survived cancer responded to the survey, of whom 5,891 (73.9%) indicated annual household income. Prostate (31.3%), colorectal (22.7%) and breast (21.8%) cancer accounted for the majority of respondents. Of those who reported household income data, over 90% wrote about the impact of physical changes following treatment, their concerns about the changes, and whether they sought help for their concerns. The most frequently identified physical challenge was fatigue (63.7%). Older survivors with low annual household incomes of less than $CA25,000 reported the highest levels of concern about multiple physical symptoms. 25% or more of the survey respondents across all income levels reported difficulty finding assistance for their concerns about the physical challenges, especially in their local communities.

Conclusion
Older survivors of cancer can experience a range of physical changes, amenable to intervention by physical therapy, yet experience challenges obtaining relevant help. Those with low income are more severely affected, even within a universal healthcare system. Financial assessment and tailored follow-up are recommended.