bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rehabilitation of water distribution networks: when and how to rehabilitate
Authors: Evangelista Leandro Alves , Brentan Bruno Melo , Lima Gustavo Meirelles 
Published: June 2023

Abstract

In this paper, a deterioration model is created and used to simulate the life cycle of a water distribution network (WDN). Then, two strategies – leakage fixing and pipe cleaning – are evaluated to rehabilitate its capacity to attend the demand. In order to implement the deterioration model, the following parameters were considered: growth of the consumer population, increase in leakage rate, functional pipe deterioration and reduction of the hydraulic capacity of the pumps. For the leakage fixing, a fixed reduction rate in water losses was considered throughout the entire WDN until a minimum reference value was reached. For pipe rehabilitation, leaning was considered at a rate of 1% of the total length of the network per year. In each of the rehabilitation strategies, a cost–benefit analysis was carried out using the net present value. The results showed that both alternatives can restore the capacity of the WDN, with the pipe cleaning presenting a better economic impact.
Modulation of phytotoxic and cytogenetic effects of cadmium by humic acid: Findings from a short-term plant-based bioassay
Authors: Samarakoon Thilomi , Fujino Takeshi 
Published: June 2023

Abstract

The study of the modulation of the toxicity of heavy metals by coexisting chemicals in the environment is vital for realistic ecological risk assessment. Our study was aimed at determining possible toxicity modulations of Cd by humic acid (HA) using the Allium cepa test system. A. cepa bulbs were exposed to Cd (1 and 5 mg/L) and HA (10 mg/L) individually or in mixtures. The root lengths of the bulbs and cytogenetic endpoints in root meristematic cells, including the mitotic index (MI), nuclear abnormalities (NAs), and chromosomal abnormalities (CAs), were determined. The results revealed that the MIs of A. cepa co-exposed to HA and Cd were significantly recovered by &gt;15% compared with those of A. cepa subjected to Cd-only treatments, and this response was more sensitive than the phytotoxic response (root length). Furthermore, the burden of NAs was significantly decreased in the co-exposed bulbs by &gt;20% compared with bulbs with Cd-only treatments. The frequencies of CAs were also reduced in the bulbs co-exposed to HA and 1 and 5 mg/L Cd by &gt;15 and &gt;25%, respectively, compared with bulbs receiving Cd-only treatments. Therefore, our findings indicated that HA plays a significant protective role in Cd toxicity in A. cepa.
Combining phthalimide innate of a positive-charge nanofiltration membrane for high selectivity and rejection for bivalent cations
Authors: Wang Zhe , Cao Jiawei , Zhang Fan , Zhang Xinbo , Tan Xinai 
Published: June 2023

Abstract

A positively charged nanofiltration (NF) membrane is known to have exceptional separation performance for bivalent cations in aqueous solutions. In this study, a new NF activity layer was created using interfacial polymerization (IP) on a polysulfone (PSF) ultrafiltration substrate membrane. The aqueous phase combines the two monomers of polyethyleneimine (PEI) and phthalimide, while successfully producing a highly efficient and accurate NF membrane. The conditions of the NF membrane were studied and further optimized. The aqueous phase crosslinking process enhances the polymer interaction, resulting in an excellent pure water flux of 7.09 L·m−2·h−1·bar−1 under a pressure of 0.4 MPa. Additionally, the NF membrane shows excellent selectivity toward inorganic salts, with a rejection order of MgCl2 &gt; CaCl2 &gt; MgSO4 &gt; Na2SO4 &gt; NaCl. Under optimal conditions, the membrane was able to reject up to 94.33% of 1,000 mg/L of MgCl2 solution at an ambient temperature. Further to assess the antifouling properties of the membrane with bovine serum albumin (BSA), the flux recovery ratio (FRR) was calculated to be 81.64% after 6 h of filtration. This paper presents an efficient and straightforward approach to customize a positively charged NF membrane. We achieve this by introducing phthalimide, which enhances the membrane's stability and rejection performance.
Osteoblast Biospecific Extraction Conjugated with HPLC Analysis for
Screening Bone Regeneration Active Components from Moutan Cortex
Authors: Yao Fei , Chen Wei , Gu Weiwei , Xu Heng , Hou Wenyue , Liang Guoqiang , Zhang Zhu Ruixian , Jiang Guorong , Zhang Lurong 
Published: June 2023

Introduction:
The function of promoting bone regeneration of Moutan Cortex (MC), a
traditional Chinese medicine, has been widely known but, the effective components of MC in promoting
osteoblast-mediated bone regeneration were still unclear.

Objective:
The method of osteoblast membrane bio-specific extraction conjugated with HPLC
analysis was established to screen bone regeneration active components from MC.

Methods:
The fingerprints, washing eluate and desorption eluate of MC extract were analyzed by
the established HPLC-DAD method. The established MC3T3-E1 cells membrane chromatography
method was used for the bio-specific extraction of MC. The isolated compounds were identified
by MS spectrometry. The effects and possible mechanisms of the isolated compounds were evaluated
by molecular docking, ALP activity, cell viability by MTT Assay and proteins expression by
Western Blot Analysis.

Results:
The active compound responsible for bone regeneration from MC was isolated using the
established method of osteoblast membrane bio-specific extraction conjugated with HPLC analysis,
and it was identified as 1,2,3,4,6-penta-O-β-galloyl-D-glucose (PGG) by MS spectrometry. It
was further demonstrated through molecular docking that PGG could fit well into the functional
ALP, BMP2, and Samd1 binding pocket. The proliferation of osteoblasts was promoted, the level
of ALP was increased, and the protein expression of BMP2 and Smad1 was increased as shown by
further pharmacological verification.

Conclusion:
It was concluded that PGG, the bone regeneration active compound from MC, could
stimulate the proliferation of osteoblasts to promote osteoblast differentiation, and its mechanism
might be related to the BMP/Smad1 pathway.
Metabolism Pathways of Major Therapeutics for Treating Monkeypox Mono- and
Co-infection with Human Immunodeficient Virus or SARS-CoV-2
Authors: Yan Daisy , Yan Bingfang 
Published: June 2023

Abstract:
Monkeypox is a zoonotic viral disease and remains endemic in tropical regions of Central and West Africa.
Since May of 2022, cases of monkeypox have soared and spread worldwide. Confirmed cases have shown no
travel history to the endemic regions as seen in the past. The World Health Organization declared monkeypox a
global public health emergency in July 2022, and the United States government followed suit one month later. The
current outbreak, in contrast to traditional epidemics, has high coinfection rates, particularly with HIV (human immunodeficiency
virus), and to a lesser extent with SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2),
the pathogen of COVID-19. No drugs have been approved specifically for monkeypox. However, there are therapeutic
agents authorized to treat monkeypox under the Investigational New Drug protocol, including brincidofovir,
cidofovir, and tecovirimat. In contrast to limited options for monkeypox treatment, there are available drugs specifically
for HIV or SARS-CoV-2 infection. Interestingly, these HIV and COVID-19 medicines share metabolism pathways
with those authorized to treat monkeypox, particularly of hydrolysis, phosphorylation, and active membrane
transport. This review discusses how these pathways shared by these medicines should be considered to gain therapeutic
synergy and maximize safety for treating monkeypox coinfections.
Gardenin B, A Natural Inhibitor for USP7: In vitro Evaluation and In silico Identification
Authors: Zhang Siyu , Sun Yujie , Bai Zhongyue , Wang Yifan , Zhao Guangjian , Yao Fengli , Yang Yacong , Hu Yu , Li Xionghao , Liu Fang , Wang Peng , Xu Ximing 
Published: June 2023

Background:
Ubiquitin-specific protease 7 (USP7) is one of the most widely studied deubiquitin
enzymes (DUBs). The protein level of USP7 is highly expressed in a variety of malignant cancers,
which suggests that it is a prognostic marker of cancers and a potential drug target for oncotherapy.

Objective:
The aim of this study was to identify natural and effective USP7 inhibitors, in order to understand
the activation of the USP7/p53 pathway by natural inhibitors.

Methods:
In this study, USP7 enzyme activity screening assay system and p53 luciferase reporter assay
system have been applied for the discovery of natural USP7 inhibitors targeting the catalytic active site.
Molecular docking and molecular dynamics (MD) simulation revealed the combined mechanism between
USP7 with gardenin B.

Results:
The gardenin B was screened from our home-lab natural products (160 flavonoids) and had cytotoxicity
in HCT116 cells (IC50 = 46.28 ± 2.16 μM). Preliminary in vitro studies disclosed its antiproliferative
activity and activated p53 signaling pathway in HCT116 cells. We found that the complex formed by
gardenin B and 5vsk (Ledock score = -6.86, MM/GBSA score = -53.35) had the optimal binding conformation.
Moreover, the MD simulation showed that the π-π interactions between gardenin B with His461
and Phe409, and the hydrogen bonds interaction between gardenin B with Leu406 played an important
role in maintaining the close binding of the complexes.

Conclusion:
In conclusion, gardenin B could be used as a natural product inhibitor of USP7 for further
optimized design and development potential.
Obstacles and Threats for Developing Entrepreneurship Ecosystem in a
Developing Country: Iran
Authors: Khosravi-Darani Koroush , Khosravi-Darani Kianoush 
Published: June 2023

Abstract:
The development of entrepreneurship which is a requirement for the commercialization
of academic findings in each society depends on many influencing factors. In this paper, these influencing
variables on the entrepreneurial ecosystem in IRAN (in general and with a special focus
on food technology) have been discussed. Furthermore, the ecosystem, and situations of the food
industry in Iran as well as the barriers to the development of entrepreneurship have been described.
After a review of the key success of succeeding countries, then, obstacles and approaches in Iran
have been described. Also, the method and manner of ecosystem development, as well as effective
components in the entrepreneurship ecosystem are introduced. Finally, the key success factors of
the entrepreneurial ecosystem and evaluation criteria are described. Both academic and food industries
may benefit from this study, especially start-ups, which want to start a business in this ecosystem
and similar ones. Based on previous experiences a questionnaire has been recommended for the
development of a new network, e.g. food technology network.
A Review on Imaging Techniques and Artificial Intelligence Models for
Osteoporosis Prediction
Authors: Inigo S.Arun , Tamilselvi R. , Beham M.Parisa 
Published: June 2023

Abstract:
Osteoporosis causes harmful influences on both men and women of all races. Bone mass, also referred to as "bone density," is frequently used to assess the health of bone. Humans frequently experience bone fractures as a result of trauma, accidents, metabolic bone diseases, and disorders of bone strength, which are typically led by changes in mineral composition and result in conditions like osteoporosis, osteoarthritis, osteopenia, etc. Artificial intelligence holds a lot of promise for the healthcare system. Data collection and preprocessing seem to be more essential for analysis, so bone images from different modalities, such as X-ray, Computed Tomography (CT), and Magnetic Resonance Imaging (MRI), are taken into consideration that help to recognize, classify, and evaluate the patterns in clinical images. This research presents a comprehensive overview of the performance of various image processing techniques and deep learning approaches used to predict osteoporosis through image segmentation, classification, and fault detection. This survey outlined the proposed domain-based deep learning model for image classification in addition to the initial findings. The outcome identifies the flaws in the existing literature's methodology and lays the way for future work in the deep learning-based image analysis model.
Carbonated Beverages and Puffed Foods Cause Gastric Rupture: A Case
Report
Authors: Wu Jianfen , Zhang Jinshun , Yang Wei 
Published: June 2023

Introduction:
Carbonated beverages and puffed foods are popular among young people during leisure and entertainment. However, there have been a few death cases reported after ingesting large amounts of junk food in a short time.

Case Presentation:
A 34-year-old woman was admitted to the hospital with acute abdominal pain due to a bad mood and consumption of large amounts of carbonated beverages and puffed foods. Emergency surgery revealed a ruptured dilated stomach combined with severe abdominal infection, and the patient died after the surgery.

Conclusion:
We should keep in mind the possibility of gastrointestinal perforation in patients with acute abdomen with a history of heavy consumption of carbonated beverages and puffed foods. We need to evaluate the acute abdomen patients after taking large amounts of carbonated beverages and puffed foods in combination with symptoms, signs, inflammatory indicators, imaging and other examinations, and the possibility of gastric perforation needs to be considered, and emergency repair surgery should be arranged.
Synthesis, Characterization, and In vitro Evaluation of Short Cationic Peptides
for Gene Delivery Vehicle Candidate
Authors: Hidayat Ace Tatang , Maharani Rani , Chaerunisaa Anis Yohana , Masduki Fifi Fitriyah , Aditama Reza , Setiawan Heri , Tarwadi Tarwadi 
Published: June 2023

Background:
Amongst gene delivery vehicles, peptide-based vectors have drawn the
intensive attraction of experts globally due to their simplicity and many advantages due to ease in
design, biocompatibility, and safety. Rationally designed peptides are capable of condensing DNA
molecules efficiently and facilitating gene expression in the target cells.

Objective:
This study aims to design, synthesize and evaluate short cationic peptides composed of
several positively charges amino acids of lysine (K) and arginine (R) for gene delivery vehicle candidates.

Methods:
The short cationic peptides of PKKKRKV (P1), CHSPKKKRKV (P2), and YGRKKRRQRRR
(P3) were synthesized using a solid-phase method on 2-chlorotrityl chloride resin.
The crude peptides were purified using RP-HPLC and characterized by HR-TOF-ESI-MS and
1H-NMR. The capability of the peptides to condense DNA was evaluated by ethidium bromide
exclusion assay. Cytotoxicity study of the peptides was carried out in HEK-293T, CHO-K1, and
HepG2 cells using MTT assay. Gene expression facilitated by the peptides was determined in the
HEK-293T.

Results:
The peptides were successfully synthesized with high purity (> 90%) and in a high consistency
with the synthetic products, as shown by the spectroscopic data. Physicochemical and biological
evaluation showed that the cationic peptides are capable of condensing DNA molecule and
have low cytotoxicity to the cells of HEK-293T, CHO-K1, and HepG2. Moreover, the cationic peptides
facilitated gene delivery of green fluorescence protein more efficiently compared to PLL.

Conclusion:
The short cationic peptides rich in lysine and arginine have been successfully synthesized
using solid-phase peptide synthesis method. They were found to be capable of condensing
DNA, have low cytotoxicity, and facilitate gene delivery. However, structure modification or formulation
of cationic peptide with lipid components to form cationic liposome is still needed to enhance
transgene expression by these peptides.
Brain-Derived Neurotrophic Factor – The Protective Agent Against Neurological Disorders
Authors: Koyya Prathyusha , Manthari Ram Kumar , Pandrangi Santhi Latha 
Published: June 2023

Abstract:
The burden of neurological illnesses on global health is significant. Our perception of the
molecular and biological mechanisms underlying intellectual processing and behavior has significantly
advanced over the last few decades, laying the groundwork for potential therapies for various neurodegenerative
diseases. A growing body of literature reveals that most neurodegenerative diseases
could be due to the gradual failure of neurons in the brain's neocortex, hippocampus, and various subcortical
areas. Research on various experimental models has uncovered several gene components to
understand the pathogenesis of neurodegenerative disorders. One among them is the brain-derived
neurotrophic factor (BDNF), which performs several vital functions, enhancing synaptic plasticity and
assisting in the emergence of long-term thoughts. The pathophysiology of some neurodegenerative
diseases, including Alzheimer’s, Parkinson’s, Schizophrenia, and Huntington’s, has been linked to
BDNF. According to numerous research, high levels of BDNF are connected to a lower risk of developing
a neurodegenerative disease. As a result, we want to concentrate on BDNF in this article and
outline its protective role against neurological disorders.
Supercritical Fluid Extraction of Phenolics from Anisophyllea disticha (Jack) Baill. and Evaluation of their Antioxidant Activities
Authors: Ferdosh Sahena , Abdul Bari Nurul Ashikin , Wu Bulan , Sarker Md. Zaidul Islam 
Published: June 2023

Background:
Anisophyllea disticha (Jack) Baill. (A. disticha) is a species of the Anisophylleaceae family that has undergone the least investigation despite being widely used in folk medicine to cure a wide range of illnesses.

Objective:
The purpose of this study is to examine the impact of various factors on the supercritical fluid extraction of A. disticha in order to maximise recovery of total phenolic content, antioxidant activity, and polyphenol identification.

Method:
The total phenolic content (TPC) and antioxidant activities of A. disticha were determined using the supercritical fluid extraction (SFE) method and compared with Soxhlet. Box-Behnken design of response surface methodology was performed to examine the effect of independent variables of SFE such as temperature, pressure, and concentration of ethanol as co-solvent on TPC and antioxidant activities of A. disticha stem extracts.

Result:
At combined effects of different temperatures, pressure, and co-solvent, the total SFE yield were ranged between 0.65 and 4.14%, which was about half of the Soxhlet extract of 8.75±1.54%. The highest concentration (µg/g) of gallic acid (118.83±1.17), p-coumaric (61.60±0.33), ferulic acid (57.93±1.15), and quercetin (24.16±0.41) were obtained at a temperature of 50 ˚C, the pressure of 25 MPa and co-solvent of 20%, while lowest concentration was found 70 ˚C, 30 MPa, and 20% ethanol.

Conclusion:
SFE extracts possessed remarkable TPC and concentration of phenolic compounds, indicating superior recovery of compounds. SFE showed more than two-fold higher ferric-reducing antioxidant power compared to Soxhlet with values of 585.32±17.01 mg Fe (II)/g extract and 203.63±16.03 mg Fe (II)/g extract, respectively. SFE demonstrated a potential alternative to the classical solvent extraction methods.
A Mini-Review on the Structural Characteristics and Anticancer Activity
of Nagilactones
Authors: Xiao Yuchen , Yong Jianping , Lu Canzhong 
Published: June 2023

Abstract:
Natural products provide abundant resources for the development of new drugs. Podocarpus
nagi is an arbor of Podocarpus L'Hér. ex Persoon. Its fruits, leaves, and roots exhibit a
broad spectrum of pharmacological activities (such as antitumor, plant growth regulation, termite
killing, and insect larval toxicity), which have been used in Yao folk for a long history. Nagilactone
is one of the key components discovered in Podocarpus nagi, which has a variety of structures and
a broad spectrum of antitumor activities. In this mini-review, the structures and spectral characteristics,
together with the antitumor activities and the structure-activity relationships of nagilactones,
are summarized by searching the database in order to provide detailed references for researchers to
elucidate and modify their structures.
A Comprehensive Review on Chemistry and Contribution of Chinese
Herb Epimedium brevicornum Maxim in Medicine
Authors: Agrawal Neetu , Goyal Deepika , Bansal Dimple 
Published: June 2023

Background:
Traditional Chinese medicine (TCM) is a complete medical system that has been used for more than 2,000 years and it is effective to use Epimedium Brevicornum (EB) Maxim, one of the Chinese herbs belonging to the family Berberidaceae is of major use because of its bioactive compound Icariin (ICA).

Objective:
This review aims on providing a collective report of the description, taxonomy, therapeutic uses, bioactive compounds, and the different pharmacological activities of the plant EB for future research.

Methods:
Data was obtained from various informative tools like PubMed, ScienceDirect, Google Scholar, and the botanical information sites for different plants.

Results:
This literature review shows that the Chinese herb EB possesses various therapeutic effects and can be used in the prophylaxis of different ailments. The extract of different parts of EB contains many bioactive compounds such as phenols, flavonoids, and lignans. They show a wide range of pharmacological activities which include anti-inflammatory, anti-infertility, anti-cancer, and effective against Alzheimer’s disease and osteoporosis. ICA was found to be the major constituent of this herbal plant aiding in almost every pharmacological activity
.

Conclusion:
The review covers every activity the plant holds and indicates that the plant is a useful source in eradicating a variety of ailments. Researchers have performed invitro and invivo experiments to explore plant capabilities. The plant could be of very much use in botanical and pharmacological fields. For experts aiming to research EB, this review could be a great source of information.
A Critical Review of Gain Enhancement Methods for Microstrip Antenna
Authors: Quzwain Kamelia , Nabila Haifa , Anwar Radial , Ismail Alyani 
Published: June 2023

Abstract:
In the recent years, the development in communication system has grown rapidly, especially
for the growth of broadband wireless technologies. This technology has some advantages, for
instance, flexibility for the transmission of data with very high data rate communications. Antenna
is one of the crucial components of broadband wireless systems because it has ability to emit and
receive radio waves. There are various types of antennas and microstrip is one of the most popular
designs nowadays. This antenna has some advantages and disadvantages. Low gain is known as one
of the disadvantages of microstrip antenna. However, there are numerous methods which can be
used to enhance gain. Some researches have been done by numerous researchers throughout the
world in tackling this disadvantage. This paper presents a critical review of different methods employed
to alleviate this problem. For ease of understanding, this paper is classified into five approaches:
array configuration approaches, air substrate approaches, metamaterial approaches, yagiuda
approaches, and other approaches.