bluuebunny/crossref_metadata_2025_split huggingface dataset:
Auricular mucormycosis complicated by parotid abscess and facial paralysis: A very rare case report
Authors: Hammemi Bouthaina , Achour Imen , Walha Omar , Yousfi Ghada , Mnejja Malek , Charfeddine Ilhem 
Published: February 2023

AbstractMucormycosis is a serious and relatively rare invasive fungal infection. The rhino‐orbito‐cerebral localization is the most frequent. Other localizations have been reported including: cutaneous, pulmonary, disseminated, gastrointestinal, and miscellaneous. Mucormycosis of external ear with facial palsy is extremely rare and only few cases have been reported in the literature. We describe a case of mucormycosis of the external and middle in a 44‐years‐old diabetic patient which had a very aggressive form of necrotic infection of the ear auricle complicated by parotid abscess, facial, and vagal nerve paralysis. Auricular mucormycosis was suspected and the diagnosis was established after biopsy and histological examination. The patient was treated immediately with intravenous amphotericin B followed by extensive surgical debridement of the necrotic lesions. The patient responded well to the treatment despite the persistence of facial palsy.
Acute ethmoiditis complicated by intraorbital abscess, orbital cellulitis, and cerebral empyema in a 14‐year‐old girl
Authors: Colombe Moise Mbaluku , Nabuloho Erick Heri , Opondjo Fernand Manga , Bianga Viviane Feza , Rodrigue Fikiri Bavurhe , Isonga Stéphanie Sifa , Shemahamba Peniel Kundo , Birindwa Archippe Muhandule 
Published: February 2023

AbstractWe report the case of a 14‐year‐old girl with pain and protrusion of the left eye and treated with diclofenac. Clinical and paraclinical examinations revealed a cerebral empyema and a left retro‐orbital abscess complicating an acute ethmoiditis. Parenteral antibiotic remains essential in the management of acute ethmoiditis to prevent complications.
Hepatic portal venous gas in the case of superior mesenteric artery thrombosis in a young adult‐case report
Authors: Dhakal Prakash , Sharma Suraj , Sharma Abhishek , Pandey Shailendra , Aryal Sajiva , Bhandari Seema 
Published: February 2023

AbstractHepatic portal venous gas is diagnosed via computed tomography due to unusual imaging features. HPVG when linked with pneumatosis intestinalis has a high mortality rate and required urgent intervention. We present a case of a 26‐year‐old young adult with superior mesenteric artery thrombosis who presented with severe abdominal pain. On imaging, HPVG and pneumatosis intestinalis were seen owing to the urgent intervention of the patient. The reliable interpretation of the imaging findings along with quick intervention led to a favorable outcome in our case. Herein, we present a thorough review of the imaging findings of HPVG to make a reliable diagnosis when presented with such a case.
Spontaneous idiopathic spinal subdural hemorrhage in a 16‐year‐old boy: A rare case in pediatrics and review of literature
Authors: Ahrari Iman , Jamali Mohamad , Mohammadi Somayeh , Ghavipisheh Mahsa , Eghbal Keyvan , Saffarian Arash , Rahmanian Abdolkarim , Ghahramani Soulmaz , Taheri Reza 
Published: February 2023

AbstractIdiopathic spinal subdural hematoma (SSDH) is a rare phenomenon. Here, we present a 16‐year‐old‐boy who presented with acute sudden onset weakness and brown squared syndrome; the cervical MRI findings showed acute subdural hematoma from C2 to C6. Emergent surgical intervention was performed, and significant improvement was seen in follow‐ups.
Erdheim‐Chester disease: A multisystem disease case illustration with rare manifestations and treatment challenges
Authors: Jridi Maysam , Ben Achour Tayssir , Naceur Ines , Smiti Monia , Ben Ghorbel Imed , Lamloum Mounir , Said Fatma , Houman Mohamed Habib 
Published: February 2023

AbstractErdheim‐Chester disease is a rare multisystemic disease. A 50‐year‐old woman, presented with a recurrent pain and swelling of the left knee. Bone scintigraphy showed increased tracer uptake of peripheral skeleton. The computed tomography showed tissular infiltration in the retroperitoneum, around the vessels. Immunohistochemistry showed CD68 (+) and CD1a (−).
Hematuria during the right heart catheterization procedure: Renal perforation as a very rare complication
Authors: Jenab Yaser , Tofighi Saeed , Navid Hossein , Riazi Homan , Samimi Sahar 
Published: February 2023

AbstractThe occurrence of hematuria during a right heart catheterization can be a sign of renal perforation, a rare but life‐threatening complication that could be developed due to the misdirection of wire into the abdominopelvic venous plexus. We showed this complication could be managed with venoplasty of the common iliac vein.
Benocyclidine (BTCP) as Non‐labelled Reporter Ligand for MS Binding Assays for the PCP Ion Channel Binding Site of the Desensitized Torpedo Nicotinic Acetylcholine Receptor (nAChR)
Authors: Währa Mark , Allmendinger Lars , Höfner Georg , Wanner Klaus T. 
Published: February 2023

AbstractIn this study we present MS Binding Assays for the PCP ion channel binding site of Torpedo californica nicotinic acetylcholine receptor (nAChR) as an alternative to radioligand binding assays. As MS Marker Benocyclidine (BTCP) was employed, found to be more affine (Kdof 84.2 nM) than the radioligands, e. g. [3H]PCP, used so far in respective binding assays. Based on a highly sensitive and fast LC‐ESI‐MS/MS method for quantification of BTCP samples, BTCP MS Binding Assays for the PCP ion channel binding site of Torpedo nAChR could be established comprising saturation, kinetic and competition experiments. The affinities obtained in competitive BTCP MS Binding Assays for ligands addressing the PCP ion channel binding site of Torpedo nAChR were in excellent accord with those reported from radioligand experiments. Thus, the new BTCP MS Binding Assays represent a potent and reliable alternative to radioligand binding assays used so far for the characterization of ligand binding to the PCP ion channel binding site of the nAChR.
Applying a risk prediction model for bloodstream infection in a febrile, nonseverely neutropenic cohort of pediatric stem cell transplant patients
Authors: Jackson Kasey , Anderson Victoria , Zhao Zhiguo , Kitko Carrie L. , Connelly James A. , Ho Richard H. , Banerjee Ritu , Dulek Daniel E. , Friedman Debra L. , Esbenshade Adam J. 
Published: February 2023

AbstractBackgroundThe optimal management of febrile stem cell transplant (SCT) patients presenting without severe neutropenia (absolute neutrophil count [ANC] ≥ 500/µL) is unclear. The authors have developed iterative risk prediction models (Esbenshade Vanderbilt [EsVan] models) that reliably predict bloodstream infections (BSIs) in the febrile general pediatric oncology population without severe neutropenia, but SCT‐specific data are limited.MethodsAll SCTs occurring from May 2005 to November 2019 at a single institution were identified. Episodes of fever with a central venous catheter and ANC values ≥ 500/µL were abstracted. All previous versions of the EsVan model were applied to the SCT data, and c‐statistics were generated. The models were additionally applied to each type of transplant (autologous/allogeneic), and a new allogeneic model that further adjusted for metrics of immunosuppression, Esbenshade Vanderbilt Allogeneic SCT Model (EsVanAlloSCT), was developed and internally validated.ResultsFor 429 SCT episodes (221 autologous and 208 allogeneic), the BSI incidence was 19.6% (84 of 429), and it was higher in allogeneic transplant patients (25.5%) than autologous transplant patients (14.0%; p < .01). All versions of the EsVan model performed well for the overall SCT cohort (c‐statistics, 0.759–0.795). The EsVan models performed better for the autologous episodes (c‐statistics, 0.869–0.881) than the allogeneic SCT episodes (c‐statistics, 0.678–0.717). The new allogeneic transplant–specific model, EsVanAlloSCT, which added an adjustment for the extent of immunosuppression, yielded a c‐statistic of 0.792 (bootstrap‐corrected, 0.750).ConclusionsThe EsVan models work exceptionally well when they are applied to autologous SCT, but they work less well for allogeneic SCT. EsVanAlloSCT appears to improve the predictive ability in allogeneic SCT, but it will need additional external validation.
Direct Conversion of CO2 in Lime Kiln Waste Gas Catalyzed by a Copper‐Based N‐heterocyclic Carbene Porous Polymer
Authors: Rao Zhi‐Xiu , Chen Pei‐Bo , Xu Jin , Wang Qing , Tang Hai‐Tao , Liang Ying , Pan Ying‐Ming 
Published: February 2023

AbstractIndustrial waste gas is one of the major sources of atmospheric CO2, yet the direct conversion of the low concentrations of CO2 in waste gases into high value‐added chemicals have been a great challenge. Herein, a copper‐based N‐heterocyclic carbene porous polymer catalyst (Cu@NHC‐1) for the direct conversion of low concentration CO2 into oxazolidinones was successfully fabricated via a facile copolymerization process followed by the complexation with Cu(OAc)2. A continuous flow device was designed to deliver a continuous and stable carbon source for the reaction. Due to the triple synergistic effect of its porous structure, nitrogen activation sites and catalytic Cu center, Cu@NHC‐1 shows highly efficient and selective adsorption, activation, and conversion of the low concentration CO2 (30 vol%). Its practical application potential is demonstrated by the ability to successfully convert the CO2 in lime kiln waste gas into oxazolidinones in satisfactory yields under mild conditions.
Do dried blood spots have the potential to support result management processes in routine sports drug testing?—Part 3: LC–MS/MS‐based peptide analysis for dried blood spot sampling time point estimation
Authors: Brockbals Lana , Thomas Andreas , Schneider Tom D. , Kraemer Thomas , Steuer Andrea E. , Thevis Mario 
Published: February 2023

AbstractAlong with the recent acknowledgement of the World Anti‐Doping Agency to use dried blood spot (DBS) samples for routine doping control purposes, there have been propositions to use DBS as a matrix that allows regular proactive remotely supervised self‐sampling, providing potential longitudinal monitoring of an athlete's exposure to doping agents. However, several organizational aspects have to be considered before implementation, such as the verification of the sample collections time point. Based on a previous untargeted proteomics workflow utilizing liquid chromatography–high‐resolution mass spectrometry (LC–HRMS) to identify protein/peptide markers to define the time since deposition of a bloodstain, the aim of the current study was to develop a targeted LC–HRMS/MS analytical method for promising peptidic target analytes. A long‐term DBS storage experiment was carried out over a 3‐month period (sample collection time points: 0, 2, 4, 7, 14, 21, 28, 42, 56, 70, 84 and 91 days) with DBS samples of 10 volunteers for longitudinal investigation of signal abundance changes of targeted peptide sequences at different storage temperatures (room temperature [RT], 4°C and −20°C). Prior to experimental analysis, LC–HRMS/MS method characteristics were successfully assessed, including intraday precision, carryover and sample extract stability. For estimation of DBS sample collection time points, ratios of two peptides that originate from the same protein prior to tryptic digestion were created. Two targeted peptide area ratios were found to significantly increase after being stored at RT for 28 days, representing potential markers for future use in routine doping controls that contribute to advancing complementary avenues in anti‐doping.
Cell‐type profiling of the sympathetic nervous system using spatial transcriptomics and spatial mapping of mRNA
Authors: Kasemeier‐Kulesa Jennifer C. , Morrison Jason A. , McKinney Sean , Li Hua , Gogol Madelaine , Hall Kate , Chen Shiyuan , Wang Yongfu , Perera Anoja , McLennan Rebecca , Kulesa Paul M. 
Published: February 2023

AbstractBackgroundThe molecular identification of neural progenitor cell populations that connect to establish the sympathetic nervous system (SNS) remains unclear. This is due to technical limitations in the acquisition and spatial mapping of molecular information to tissue architecture.ResultsTo address this, we applied Slide‐seq spatial transcriptomics to intact fresh frozen chick trunk tissue transversely cryo‐sectioned at the developmental stage prior to SNS formation. In parallel, we performed age‐ and location‐matched single cell (sc) RNA‐seq and 10× Genomics Visium to inform our analysis. Downstream bioinformatic analyses led to the unique molecular identification of neural progenitor cells within the peripheral sympathetic ganglia (SG) and spinal cord preganglionic neurons (PGNs). We then successfully applied the HiPlex RNAscope fluorescence in situ hybridization and multispectral confocal microscopy to visualize 12 gene targets in stage‐, age‐ and location‐matched chick trunk tissue sections.ConclusionsTogether, these data demonstrate a robust strategy to acquire and integrate single cell and spatial transcriptomic information, resulting in improved resolution of molecular heterogeneities in complex neural tissue architectures. Successful application of this strategy to the developing SNS provides a roadmap for functional studies of neural connectivity and platform to address complex questions in neural development and regeneration.
Anxiety associated with palatable food withdrawal is reversed by the selective FAAH inhibitor PF‐3845: A regional analysis of the contribution of endocannabinoid signaling machinery
Authors: de Ceglia Marialuisa , Micioni Di Bonaventura Maria Vittoria , Romano Adele , Friuli Marzia , Micioni Di Bonaventura Emanuela , Gavito Ana L. , Botticelli Luca , Gaetani Silvana , de Fonseca Fernando Rodríguez , Cifani Carlo 
Published: February 2023

AbstractObjectiveConsumption of energy‐dense palatable “comfort” food can alleviate stress and negative emotions, while abrupt withdrawal from a palatable diet can worsen these symptoms, causing difficulties with adherence to weight‐loss diets. Currently, no pharmacological treatment is effective for obesity‐related anxiety, so we investigated the endocannabinoid system (ECS), and specifically the fatty acid amide hydrolase (FAAH), as an interesting emerging target in this context because of its key role in the regulation of both energy homeostasis and emotional behavior.MethodsRats were subjected to exposure and subsequent abstinence from a palatable cafeteria diet. During abstinence period, rats were treated with the selective FAAH inhibitor PF‐3845 (10 mg/kg; intraperitoneal administration every other day).ResultsAbstinent rats displayed an anxiogenic‐like behavior and changes in the proteins of ECS signaling machinery in brain areas involved both in anxiety and food intake regulation. In particular, withdrawal caused a reduction of the expression of cannabinoid receptors in the nucleus accumbens and of enzymes diacylglycerol lipase alpha and monoacylglycerol lipase (MAGL) in the amygdala. Pharmacological inhibition of FAAH exerted an anxiolytic‐like effect in abstinent animals and increased both MAGL expression in amygdala and CB2 expression in prefrontal cortex.DiscussionOverall, our results suggest that emotional disturbances associated with dieting are coupled with region‐specific alterations in the cerebral expression of the ECS and that the enhancement of the endocannabinoid signaling by FAAH inhibition might represent a novel pharmacological strategy for the treatment of anxiety related to abstinence from palatable food.Public SignificanceThe present study focused on evaluating the role of the endocannabinoid system in modulating withdrawal from naturally rewarding activities that have an impact on mood, such as feeding. The variations observed in the emotional behavior of abstinent rats was linked to neuroadaptations of the ECS in specific brain areas.
The role of readiness and confidence to change in the treatment of atypical anorexia nervosa and bulimia nervosa
Authors: Keegan Ella , Wade Tracey D. 
Published: February 2023

AbstractObjectiveAtypical anorexia nervosa (AN) is a serious eating disorder that is more common in the population than AN. Despite this, people with atypical AN are less likely to be referred or admitted for eating disorder treatment and there is evidence that they are less likely to complete or benefit from existing interventions. This study examined whether baseline readiness and/or confidence moderated outcomes from 10‐session cognitive behavioral therapy among people with atypical AN and bulimia nervosa (BN), and whether the impact of these variables differed between diagnoses.MethodsParticipants (n = 67; 33 with atypical AN) were a subset from an outpatient treatment study. Linear mixed model analyses were conducted to examine whether baseline readiness and/or confidence moderated outcomes.ResultsPeople with BN who had higher levels of readiness or confidence at baseline had steeper decreases in eating disorder psychopathology over time. There was no evidence that readiness or confidence moderated outcomes for people with atypical AN.DiscussionThis study suggests that the moderators that have been identified for other eating disorders may not apply for people with atypical AN and highlights a need for future work to routinely investigate whether theoretically or empirically driven variables moderate outcomes in this little‐understood population.Public Significance StatementPeople with bulimia nervosa with higher readiness and confidence experienced greater decreases in eating disorder symptoms than people with lower readiness and confidence when treated with cognitive behavioral therapy. These findings did not apply to people with atypical anorexia nervosa. Results demonstrate that future work is urgently required to identify helpful treatments for people with atypical anorexia nervosa as well as the variables that have a positive impact on outcomes in treatment for these individuals.
Long‐term risk stratification for hospitalized patients with acute heart failure at a single rural cardiovascular centre
Authors: Satogami Keisuke , Katayama Yosuke , Ozaki Yuichi , Taruya Akira , Taniguchi Motoki , Ota Shingo , Kuroi Akio , Shiono Yasutsugu , Tanimoto Takashi , Yamano Takashi , Kitabata Hironori , Ino Yasushi , Tanaka Atsushi 
Published: February 2023

AbstractAimsEarly risk stratification of patients with acute heart failure (AHF) is critical for appropriate triage and outcome improvement. The serum sodium, blood urea nitrogen, age, serum albumin, systolic blood pressure, and natriuretic peptide level (SOB‐ASAP) score can predict in‐hospital mortality of AHF. However, the relationship between the SOB‐ASAP score and long‐term prognosis is unknown. This study investigated the determinants of the long‐term prognosis of AHF by evaluating the SOB‐ASAP score.MethodsThis retrospective cohort study included all patients with acute decompensated heart failure who were admitted to our hospital between April 2017 and March 2018. And those who discharged were analysed retrospectively. The follow‐up period was 3 years. Primary end point was all‐cause death.ResultsTotal of 140 patients were analysed. The median SOB‐ASAP score of entire cohort on admission was 3 points (interquartile range; 1–5). The Kaplan–Meier curve demonstrated that patients in the higher SOB‐ASAP group (score ≥3) had a higher incidence of all‐cause death (log‐rank test; P < 0.001) than those in the lower SOB‐ASAP (group score <3).ConclusionsAt admission, the SOB‐ASAP score may be useful for predicting the long‐term prognosis of hospitalized patients with AHF.
Fibrosis‐4 index identifies worsening renal function associated with adverse outcomes in acute heart failure
Authors: Tsuda Kosuke , Maeda Daichi , Kanzaki Yumiko , Sakane Kazushi , Okuno Takahiro , Nakayama Sayuri , Tokura Daisuke , Hasegawa Hitomi , Hoshiga Masaaki 
Published: February 2023

AbstractAimsWorsening renal function (WRF) often develops during heart failure (HF) treatment. However, prognostic implications of WRF in acute HF remain controversial, and risk stratification of WRF is challenging. Although the fibrosis‐4 index (FIB‐4) was initially established as a liver fibrosis marker, recent studies show that high FIB‐4 is associated with venous congestion and poor prognosis in acute HF. This study aimed to evaluate whether FIB‐4 could identify prognostically relevant and non‐relevant WRF in patients with acute HF.Methods and resultsWe retrospectively analysed data from a single‐centre registry on acute HF at our university hospital between January 2015 and June 2021. This study included patients with acute HF aged ≥20 years who were immediately hospitalized and had brain natriuretic peptide levels ≥100 pg/mL at admission. WRF was defined as increases of ≥0.3 mg/dL and >25% in serum creatinine level from admission to discharge. FIB‐4 scores were calculated before discharge. The primary endpoint was all‐cause mortality within 1 year of discharge. Based on the presence of WRF and whether FIB‐4 scores were above the median, patients were stratified into four groups: no WRF and lower FIB‐4 scores, no WRF and higher FIB‐4 scores, WRF and lower FIB‐4 scores, and WRF and higher FIB‐4 scores. The patients were followed up via clinical visits or telephone interviews. Clinical outcomes were collected from the electronic medical records.ResultsOf the 969 patients hospitalized for acute HF (76 ± 11 years, 59% men), 118 patients (12%) had WRF at discharge. The median (interquartile range) FIB‐4 score at discharge was 2.36 (1.55–3.25). The primary endpoint occurred in 136 patients (14.0%). The 1 year mortality rates were 10.5% in the no WRF and lower FIB‐4 scores (≤2.36) group (n = 428), 16.1% in the no WRF and higher FIB‐4 scores (>2.36) group (n = 423), 12.5% in the WRF and lower FIB‐4 scores group (n = 56), and 25.8% in the WRF and higher FIB‐4 scores group (n = 62) (P = 0.005). Kaplan–Meier analysis demonstrated higher all‐cause mortality in the WRF and higher FIB‐4 group (log‐rank P = 0.003). In the Cox regression analysis, only the WRF and higher FIB‐4 scores group was associated with an increased risk of mortality compared with the no WRF and lower FIB‐4 scores group (hazard ratio = 2.11, 95% confidence interval: 1.07–4.18, P = 0.032), despite adjusting for other confounding factors.ConclusionsFIB‐4 is a valuable risk stratification marker for WRF in patients with acute HF. The underlying mechanism and potential clinical importance of these observations require further investigation.
The effects of alginate lyase treatment on structural characteristics and anti‐photoaging activity of alginate from Sargassum fusiforme
Authors: Chang Shiyuan , Hu Jinhong , Zhu Biyang , You Lijun , Pedisić Sandra , Hileuskaya Kseniya 
Published: February 2023

AbstractAn alginate (named P1) prepared from Sargassum fusiforme has been proven to have significant anti‐photoaging activity in our previous study. In this study, alginate lyase was used to obtain P1 hydrolysates with different molecular weights, and their structural characteristics and anti‐photoaging activity were compared. Results showed that alginate lyases could rapidly degrade the molecular weight of P1 and slightly changed its chemical composition. Fourier transform infrared spectra indicated that alginate lyase treatment did not change the main functional groups of P1. Moreover, the inhibitory effects of P1 on MMPs (MMP‐1, MMP‐3 and MMP‐9) and reactive oxygen species were also improved after hydrolyzed with alginate lyase, especially sample A‐30 (hydrolysis for 30 min). Methylation and nuclear magnetic resonance results indicated that the main chain of A‐30 was →4)‐β‐ManA‐(1→4)‐α‐GulA‐(1→4)‐β‐ManA‐(1→4)‐β‐ManA‐(1→4)‐α‐GulA‐(1→4)‐β‐ManA‐(1→3, 4)‐β‐ManA‐(1→, with the end group of α‐Fucp‐(1→ attached to the O‐3 position of →3, 4)‐β‐ManA‐(1→ on the side chain. It indicated that alginate lyase did not change the backbone of P1 but decreased the molecular or degree of polymerization at 30 min of hydrolysis. This is the first report on the anti‐photoaging activity of alginate hydrolysates. The results will provide effective theoretical support for improving the utilization and promoting the application of S. fusiforme.