bluuebunny/crossref_metadata_2025_split huggingface dataset:
Best practice guidelines for environmental DNA biomonitoring in Australia and New Zealand
Authors: De Brauwer Maarten , Clarke Laurence J. , Chariton Anthony , Cooper Madalyn K. , de Bruyn Mark , Furlan Elise , MacDonald Anna J. , Rourke Meaghan L. , Sherman Craig D. H. , Suter Leonie , Villacorta‐Rath Cecilia , Zaiko Anastasija , Trujillo‐González Alejandro 
Published: February 2023

AbstractEnvironmental DNA (eDNA)‐based methods are increasingly used by government agencies to detect pests and threatened species, and for broader biodiversity monitoring. Given rapid technological advances and a growing number of commercial service providers, there is a need to standardize methods for quality assurance and to maintain confidence in eDNA‐based results. Here, we introduce two documents to provide best‐practice guidelines for Australian and New Zealand eDNA researchers and end‐users (available from https://sednasociety.com/publications): the Environmental DNA protocol development guide for biomonitoring provides minimum standard considerations for eDNA and environmental RNA projects across the complete workflow, from ethical considerations and experimental design to interpreting and communicating results. The Environmental DNA test validation guidelines outline key steps to be used in assay development and validation for species‐specific testing and metabarcoding. Both guidelines were developed as an initiative of the Australian Government Department of Agriculture, Fisheries and Forestry and led by the Southern eDNA Society in a collaborative process including multiple consultation rounds with eDNA experts, end‐users, and stakeholders to adapt the guidelines to Australian and New Zealand needs. The aim of these guidelines is not to be prescriptive, but to set minimum standards to support a consistent and best‐practice approach to eDNA testing. We anticipate that the guidelines will be reviewed and regularly updated as required. Our aspiration is that these best‐practice guidelines will ensure environmental managers are provided with robust scientific evidence to support decision‐making.
Hypocapnia is an independent predictor of in‐hospital mortality in acute heart failure
Authors: Tang Wen‐Jing , Xie Bai‐Kang , Liang Wei , Zhou Yan‐Zhao , Kuang Wen‐Long , Chen Fen , Wang Min , Yu Miao 
Published: February 2023

AbstractAimsAcute heart failure (AHF) poses a major threat to hospitalized patients for its high mortality rate and serious complications. The aim of this study is to determine whether hypocapnia [defined as the partial pressure of arterial carbon dioxide (PaCO2) below 35 mmHg] on admission could be associated with in‐hospital all‐cause mortality in AHF.Methods and resultsA total of 676 patients treated in the coronary care unit for AHF were retrospectively analysed, and the study endpoint was in‐hospital all‐cause mortality. The 1:1 propensity score matching (PSM) analysis, Kaplan–Meier curve, and Cox regression model were used to explore the association between hypocapnia and in‐hospital all‐cause mortality in AHF. Receiver operating characteristic (ROC) curve and Delong's test were used to assess the performance of hypocapnia in predicting in‐hospital all‐cause mortality in AHF. The study cohort included 464 (68.6%) males and 212 (31.4%) females, and the median age was 66 years (interquartile range 56–74 years). Ninety‐eight (14.5%) patients died during hospitalization and presented more hypocapnia than survivors (76.5% vs. 45.5%, P < 0.001). A 1:1 PSM was performed between hypocapnic and non‐hypocapnic patients, with 264 individuals in each of the two groups after matching. Compared with non‐hypocapnic patients, in‐hospital mortality was significantly higher in hypocapnic patients both before (22.2% vs. 6.8%, P < 0.001) and after (20.8% vs. 8.7%, P < 0.001) PSM. Kaplan–Meier curve showed a significantly higher probability of in‐hospital death in patients with hypocapnia before and after PSM (both P < 0.001 for the log‐rank test). Multivariate Cox regression analysis showed that hypocapnia was an independent predictor of AHF mortality both before [hazard ratio (HR) 2.22; 95% confidence interval (CI) 1.23–3.98; P = 0.008] and after (HR 2.19; 95% CI 1.18–4.07; P = 0.013) PSM. Delong's test showed that the area under the ROC curve was improved after adding hypocapnia into the model (0.872, 95% CI 0.839–0.901 vs. 0.855, 95% CI 0.820–0.886, P = 0.028). PaCO2 was correlated with the estimated glomerular filtration rate (r = 0.20, P = 0.001), left ventricular ejection fraction (r = 0.13, P < 0.001), B‐type natriuretic peptide (r = −0.28, P < 0.001), and lactate (r = −0.15, P < 0.001). Kaplan–Meier curve of PaCO2 tertiles and multivariate Cox regression analysis showed that the lowest PaCO2 tertile was associated with increased risk of in‐hospital mortality in AHF (all P < 0.05).ConclusionsHypocapnia is an independent predictor of in‐hospital mortality for AHF.
Six years of fluvial response to a large dam removal on the Carmel River, California, USA
Authors: East Amy E. , Harrison Lee R. , Smith Douglas P. , Logan Joshua B. , Bond Rosealea M. 
Published: February 2023

AbstractMeasuring river response to dam removal affords a rare, important opportunity to study fluvial response to sediment pulses on a large field scale. We present a before–after/control–impact study of the Carmel River, California, measuring fluvial geomorphic and grain‐size evolution over 8 years, six of which postdated removal of a 32 m‐high dam (one of the largest dams removed worldwide) and included 11 flow events exceeding the 2‐year flood magnitude. We find that the reservoir‐sediment pulse following dam removal was relatively small (97 000 ± 24 000 t over 4 years), owing to deliberate reservoir‐sediment stabilization. Scaled to the size of the Carmel River watershed and compared against long‐term bedrock denudation rates, the post‐dam‐removal sediment release was slightly less than the annualized long‐term sediment export from this basin. New sediment transited >30 km to the river mouth in less than 2 years, assisted by floods 2 and 4 years after dam removal. The sediment pulse fined the downstream riverbed while causing mostly low‐magnitude bed‐elevation changes: commonly 0.5 to 1 m or smaller, occurring as discontinuous sediment patches or interstitial deposits, aside from the filling and subsequent partial scour of deep pools. There was no major geomorphic reset downstream from the dam site. Geomorphic changes were driven almost entirely by flow rather than by the modest increase in sediment supply, in contrast to recent examples from other large dam removals. The relatively minor disturbance caused by dam removal on the Carmel River is likely analogous to many future dam removals: a relatively small sediment pulse after deliberate limitation of reservoir‐sediment erosion, and with an upstream dam remaining in place. Thus, a large dam removal need not lead to major downstream impacts.
Metal Template Synthesis of ‘Broken’ Aromatic Preorganized Terdentate Hosts for the Recognition of Lanthanide Tris‐β‐Diketonate Guests
Authors: Le‐Hoang Giau , Guénée Laure , Naseri Soroush , Besnard Céline , Piguet Claude 
Published: February 2023

AbstractThe binding of the terdentate precursor 2,2′‐(4‐methyl‐3,5‐divinylpyridine‐2,6‐diyl)bis(1‐allyl‐5‐bromo‐1H‐benzo[d]imidazole) (1) to the lanthanide container [Ln(hfac)3] (Ln=La, Eu, Gd, Y, Er; H‐hfac=1,1,1,5,5,5‐hexafluoropentane‐2,4‐dione) ensures the cis‐cis orientation of the two adjacent α,α′‐diimine units that is required for the successful intramolecular Grubb ring‐closing metathesis generating the target rigid 6‐methyl‐9,11‐dihydro‐1H,3H‐2λ2,10λ2‐pyrido[2,3‐c:6,5‐c′]bis(azepine) scaffold decorated with two terminal 5‐bromo‐1H‐benzo[d]imidazole in ligand L7. The bond valence analysis of the crystal structures of the associated nine‐coordinate adducts [L7Ln(hfac)3] (Ln=La, Eu, Gd, Er, Y) reveals a satisfying match between the rigid terdentate cavity and the size of the bound lanthanide metal, with a pronounced preference for the largest lanthanum cation. Thermodynamic studies in dichloromethane confirm the formation of [L7Ln(hfac)3] adducts with unprecedented stabilities due to the removal of the energy penalty associated with trans‐trans to cis‐cis reorganization. The introduction of saturated methylene groups within the polyaromatic ligand backbone breaks extended aromatic delocalization and clears the visible part of the electromagnetic spectrum from emission arising from low‐energy ligand‐based excited states.
Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross‐sectional study
Authors: Kini Priscilla , Wireko Solomon , Osei‐Poku Priscilla , Asiedu Samuel O. , Amewu Emmanuel K. A. , Asiedu Ebenezer , Amanor Ernest , Mensah Caleb , Wilson Mary B. , Larbi Amma , Boahen Kennedy G. , Sylverken Augustina A. , Amato Katherine R. , Kwarteng Alexander 
Published: February 2023

AbstractBackground and AimFilarial infections affect over 150 million people in the tropics. One of the major forms of filarial pathologies is lymphedema; a condition where the immune response is significantly altered, resulting in changes in the normal flora. Staphylococcus hominis, a human skin commensal, can also be pathogenic in immunocompromised individuals. Therefore, there is the possibility that S. hominis could assume a different behavior in filarial lymphedema patients. To this end, we investigated the levels of antibiotic resistance and extent of mecA gene carriage in S. hominis among individuals presenting with filarial lymphedema in rural Ghana.MethodWe recruited 160 individuals with stages I–VII lymphedema, in a cross‐sectional study in the Ahanta West District of the Western Region of Ghana. Swabs from lymphedematous limb ulcers, pus, and cutaneous surfaces were cultured using standard culture‐based techniques. The culture isolates were subjected to Matrix‐Assisted Laser Desorption/Ionization Time of Flight (MALDI‐TOF) mass spectrometry for bacterial identification. Antimicrobial susceptibility testing (AST) was performed using the Kirby–Bauer method. mecA genes were targeted by polymerase chain reaction for strains that were cefoxitin resistant.ResultsIn all, 112 S. hominis were isolated. The AST results showed resistance to chloramphenicol (87.5%), tetracycline (83.3%), penicillin (79.2%), and trimethoprim/sulphamethoxazole (45.8%). Of the 112 strains of S. hominis, 51 (45.5%) were resistant to cefoxitin, and 37 (72.5%) of the cefoxitin‐resistant S. hominis haboured the mecA gene.ConclusionThis study indicates a heightened level of methicillin‐resistant S. hominis isolated among filarial lymphedema patients. As a result, opportunistic infections of S. hominis among the already burdened filarial lymphedema patients in rural Ghana may have reduced treatment success with antibiotics.
Relationships between Obesity and Incidence of Fractures in a Middle‐Aged Population: A Study from the CARTaGENE Cohort
Authors: Turcotte Anne‐Frédérique , Jean Sonia , Morin Suzanne N. , Mac‐Way Fabrice , Gagnon Claudia 
Published: February 2023

ABSTRACTThe association between obesity and fracture risk is complex and may vary by definition of obesity, skeletal site, and sex. We aimed to evaluate the relationships between obesity, defined using body mass index (BMI) or waist circumference (WC), and fracture incidence at any site and by skeletal site (i.e., major osteoporotic fractures [MOFs], distal lower limb fractures [tibia, ankle, feet], and distal upper limb fractures [forearm/elbow, wrist]). The secondary aim was to assess the aforementioned relationships by sex. We used CARTaGENE, a large population‐based cohort of individuals aged 40–70 years from Quebec, Canada, who were assessed in 2009–2010. Incident fractures were identified via linkage with healthcare administrative databases over a 7‐year period. Cox proportional hazard models adjusted for several potential confounders were used to estimate the relationships, with exposures treated as continuous variables. Results are reported as adjusted hazard ratios (aHRs) and 95% confidence intervals. We identified 19 357 individuals (mean ± standard deviation: age 54 ± 8 years, BMI 27 ± 5 kg/m2, WC 94 ± 14 cm; 51.6% women). During follow‐up, 497 women and 323 men sustained a fracture. There was a linear relationship between fracture incidence and WC, while cubic splines best fitted the relationship for BMI. Greater WC was associated with an increased risk of fracture at the distal lower limbs in the whole cohort and in the subgroup of women: aHR for each 10 cm increased in WC of 1.12 (1.03, 1.21) and 1.12 (1.01, 1.24), respectively. In men, WC was not significantly associated with any fracture outcome. Higher BMI was also significantly associated with distal lower limb fracture risk in the whole cohort (p = 0.018). No significant relationships were found between either WC or BMI and the risk of any fracture, MOFs, and distal upper limb fractures. In middle‐aged individuals, obesity, and mainly abdominal obesity, was associated with distal lower limb fracture risk. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Hypoxia promotes EV secretion by impairing lysosomal homeostasis in HNSCC through negative regulation of ATP6V1A by HIF‐1α
Authors: Wang Xiaoning , Wu Ruoyi , Zhai Peisong , Liu Zheqi , Xia Ronghui , Zhang Zhen , Qin Xing , Li Chuwen , Chen Wantao , Li Jiang , Zhang Jianjun 
Published: February 2023

AbstractTumour cells under hypoxia tend to modulate the number and contents of extracellular vesicles (EVs) to regulate the tumour microenvironment (TME) and thus promote tumour progression. However, the mechanism of how hypoxia influences the secretion of EVs remains to be elucidated. Here, we confirm the increased production of EVs in head and neck squamous cell carcinoma (HNSCC) cells under hypoxia, where endosome‐derived EVs are the main subtype affected by insufficient O2. The accumulation of hypoxia‐inducible factor‐1α (HIF‐1α) under hypoxia directly downregulates the expression of ATP6V1A, which is pivotal to maintain the homeostasis of lysosomes. Subsequently, impaired lysosomal degradation contributes to the reduced fusion of multivesicular bodies (MVBs) with lysosomes and enables the secretion of intraluminal vesicles (ILVs) as EVs. These findings establish a HIF‐1α‐regulated lysosomal dysfunction‐EV release axis and provide an exquisite framework to better understand EV biogenesis.
Role of psychological background in cancer susceptibility genetic testing distress: It is not only about a positive result
Authors: López‐Fernández Adrià , Villacampa Guillermo , Salinas Mònica , Grau Elia , Darder Esther , Carrasco Estela , Solanes Ares , Velasco Angela , Torres Maite , Munté Elisabet , Iglesias Silvia , Torres‐Esquius Sara , Tuset Noemí , Diez Orland , Lázaro Conxi , Brunet Joan , Corbella Sergi , Balmaña Judith 
Published: February 2023

AbstractClinical and familial factors predict psychological distress after genetic testing for cancer susceptibility. However, the contribution of an individual's psychological background to such distress is unclear. This study aims to analyze the psychological impact of genetic testing and to identify the profile of individuals at higher risk. This is a longitudinal multicenter study of individuals undergoing genetic testing for cancer susceptibility. Demographic, clinical, genetic, familial, and psychological (personality types, cancer worry) characteristics were assessed by validated questionnaires the day of genetic testing. Distress, uncertainty, and positive experience perception (MICRA scale) were evaluated at the results disclosure visit, and 3 and 12 months afterwards. Multivariate analysis was performed. A total of 714 individuals were included. A high neuroticism score, high baseline cancer worry, and a positive genetic test result were independently associated with higher psychological impact (p‐value < 0.05). The highest risk profile (10% of the cohort) included women with high level of neuroticism and a positive result. Uncertainty was mainly associated with a high level of neuroticism, regardless of the genetic test result. A holistic approach to personalized germline genetic counseling should include the assessment of personality dimensions.
Suppressed Self‐Reduction of Manganese in Mg2SnO4 via Li+ Incorporation with Polychromatic Luminescence for Versatile Applications
Authors: Xue Junpeng , Hu Tao , Li Fuqiang , Liu Fengwu , Noh Hyeon Mi , Lee Bo Ram , Choi Byung Chun , Park Sung Heum , Jeong Jung Hyun , Du Peng 
Published: February 2023

AbstractHerein, the self‐reduction behavior of manganese (Mn) in Mg2SnO4 (MSO) host lattices prepared by using the solid‐state reaction technique in air atmosphere is reported. Excited by 282 nm, only the emission of Mn2+ is seen, and the observed results are confirmed by theoretical calculation based on the density functional theory. Noteworthy, with the codoping of Li+, the self‐reduction process of Mn is suppressed, leading to the coexistence of Mn2+ and Mn4+ in MSO host lattices along with the multicolor emissions. For the suppressed self‐reduction behavior, possible hypotheses are proposed to elucidate the corresponding mechanisms. Furthermore, benefiting from the different thermal quenching behaviors of Mn2+ and Mn4+, ratiometric optical thermometers with high sensitivity of 6.59% K−1 are designed. Moreover, the prepared phosphors possess a good green afterglow phenomenon, in which their trap depth are 0.71 and 0.91 eV. In addition, via using the designed phosphors, applications in anti‐counterfeiting, fingerprint identification, and optical information storage are also realized.
Fractional carbon dioxide vaginal laser treatment of stress urinary incontinence: Remodeling of vaginal tissues and improving pelvic floor structures
Authors: Gao Lin , Wang Yuanli , Wen Wei , Duan Yunyan , Li Zhaoyang , Dang Erle , Li Peng , Yu Lei , Zhou Chenxi , Lu Meiheng , Wang Gang 
Published: February 2023

AbstractObjectiveTo demonstrate remodeling of vaginal biomechanical and physiological properties using vaginal fractional carbon dioxide (CO2) laser treatment of stress urinary incontinence (SUI).Materials and MethodsThe study cohort included 26 patients with SUI between October 2019 and November 2020. Patients were treated with two sessions of FemTouch vaginal fractional CO2 laser with a one‐month interval. Three subjective assessments were administered to all patients: female sexual function index (FSFI), vaginal health index score (VHIS), and international consultation on incontinence questionnaire‐short form (ICIQ‐SF). Vaginal tissue biopsies were taken from 6 patients before treatment and one‐month after the final treatment. Vaginal tactile imaging (VTI) measurements, ultrasonography, and magnetic resonance imaging (MRI) scans were performed before treatment and 10–12‐months after treatment in 10, 9, and 6 patients, respectively.ResultsThe average age of the cohort was 39.5 ± 12.0 years. The overall scores for FSFI, VHIS, and ICIQ‐SF significantly improved in patients after each treatment sessions as compared with baseline scores. VTI showed significantly increased pressure resistance of both the anterior and posterior vaginal walls after treatment. Ultrasonography showed significant decreases in bladder neck mobility and urethrovesical angle during the Valsalva maneuver after treatment. MRI scans showed significant decreases in the length of the vaginal anterior wall after treatment. Histological examination confirmed that the laser treatment led to a thicker stratified squamous epithelium layer as compared to the baseline.ConclusionsOur results demonstrated that vaginal fractional CO₂ laser treatment can restore vaginal biomechanical and physiological properties by increasing vaginal tightening and improving pelvic floor structures.
Expert Consensus on the Use of On‐Demand Treatments for OFF Episodes in Parkinson's Disease: A Modified Delphi Panel
Authors: Isaacson Stuart H. , Achari Madhureeta , Bhidayasiri Roongroj , Comella Cynthia , Farmer Jill Giordano , Gupta Fiona , Jones Sarah , Kreitzman David , Kremens Daniel , Lewis Simon J.G. , Poewe Werner , Tolosa Eduardo , Campos Cynthia , Gibbs Sarah N. , Broder Michael S. 
Published: February 2023

ABSTRACTBackgroundOn‐demand treatments can treat OFF episodes in Parkinson's disease, however, there is limited information regarding when to prescribe them.ObjectiveDevelop expert consensus to determine appropriate clinical factors for considering on‐demand treatments.MethodsUsing a RAND/UCLA modified Delphi panel method, a panel developed consensus on the use of on‐demand treatments for OFF episodes.ResultsThe panel agreed on‐demand treatments were appropriate when OFF episodes were associated with greater functional impact and interfered with basic daily activities. The panel also agreed on‐demand treatment may be appropriate for patients with morning akinesia and/or delayed ON of first levodopa dose and >1 type of OFF episode (eg, early morning OFF or wearing OFF regardless of frequency).ConclusionsExperts agreed on‐demand treatment is appropriate for many patients with OFF episodes. The greater the functional impact of OFF episodes, the more likely experts agreed that on‐demand treatment is appropriate to prescribe.