bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ameliorative effect of Silk Fibroin against 5-Fluorouracil (5-FU)-induced gastrointestinal damage in rats
Authors: Şehirli Ahmet Özer , Aykaç Aslı , Özkayalar Hanife , Savtekin Gökçe , Sayıner Serkan , Sayıner Serkan 
Published: September 2023

Objective: 5-Fluorouracil (5-FU), a widely administered anti-cancer drug, causes gastrointestinal damage through various mechanisms. This study aimed to investigate the ameliorative effect of silk fibroin (SF), which has anti-inflammatory and antioxidant properties, against 5 FU-induced gastrointestinal damage.
Materials and methods: Wistar albino rats were divided into 3 groups; Control group, 5- FU group (30mg/kg/day on days 0, 2, 4), and 5-FU+SF (30 mg/kg/day 5-FU on days 0, 2, 4 + 600 mg/kg/day SF for 14 days). At the end of the experiment, stomach and intestinal tissue samples were collected and immunohistochemically iNOS, caspase-3, CD4+ and CD8+ were measured. Besides, structural damage was assessed with hematoxylin-eosin and caspase-3 and 9 activities were evaluated using western blot analysis.
Results and Discussion: It was determined that iNOS, caspase-3, caspase-9 activities, CD4+ and CD8+ levels and structural damage significantly increased in both stomach and intestinal tissues. Furthermore, elevated markers were regressed almost to control levels in SF administrated group.
Conclusion: SF may have an ameliorative effect, thus reducing the gastrointestinal damage caused by 5 FU. More clinical and scientific research should be conducted as the silk fibroin may be a suitable candidate against the adverse effects of chemotherapeuic agents.
Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page : 907-915
The Association of Allergy and Systemic Lupus Erythematosus as a Single Disease and an Overlapping Syndrome compared to Control Group
Authors: Nareswari Kirana Pawitra , Werdiningsih Yulyani , Pratiwi Dewi , Aryani Niken Dyah , Matea Ari 
Published: September 2023

Background:Allergic diseases and systemic lupus erythematosus (SLE) sometimes are being connected since both of these diseases are affected by gene-environment processes. SLE can appear coexisting with other autoimmune diseases as an overlapping syndrome.
Objectives: This research was done to investigate the relation of allergic diseases and SLE as a single SLE or overlapping syndrome compared to control.
Method: We collected data from 39 SLE only, 22 overlapping syndrome SLE, and 39 non SLE subjects who had filled an online Score for Allergic Rhinitis (SFAR) questionnaire.
Results: There are significant outcomes of the existence of allergic disease (p=.007), atopic dermatitis (p=.000) and total number of allergies (p=.016) in SLE patients. There is a correlation of the appearance of allergy in SLE as a single or coexisting with other autoimmune disease compared to control group.
Conclusion: Atopic dermatitis and the more number of allergic diseases that the one have, play role in appearing SLE.
Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page : 916-919
Pemanfaatan Tumbuhan Air Purun Tikus (Eleocharis dulcis) Kelompok Usaha Wanita Pengrajin Purun ‘Galoeh Badjar’
Authors: Yunita Rizmi , Sofarini Dini 
Published: September 2023

Kelompok usaha wanita pengrajin purun merupakan salah satu kelompok pengrajin purun di kelurahan Palam yang telah mengembangkan bahan baku menjadi produk kerajinan dengan bahan utama tumbuhan air purun tikus. Kelompok pengrajin purun dalam perkembangannya menghadapi permasalahan dalam keterbatasan pengetahuan tentang pemilihan dan pemanfaatan tumbuhan air purun tikus yang baik, penanganan pewarnaan. Solusi yang ditawarkan adalah pengrajin dapat membedakan purun tikus dengan mutu yang baik digunakan sebagai bahan dasar anyaman, pemilihan bahan dasar, pewarnaan bahan dasar, agar meningkatkan mutu bahan dasar anyaman. Metode kegiatan Pengabdian Kepada Masyarakat (PKM) yang diterapkan, yaitu: 1) Konsultasi berupa pertemuan, diskusi, dan penyampaian materi, dan 2) Pelatihan berupa demonstrasi dan redemontrasi dan evaluasi kegiatan. Hasil kegiatan PKM memberikan adanya kondisi perubahan dalam menambah wawasan pengetahuan dan mau melaksanakan apa yang sudah diinformasikan kepada kelompok mitra tentang penggunaan bahan dasar yang baik dan pewarnaan bahan dasar agar mampu mengembangkan usaha sebagai suatu bisnis yang mandiri. Pertemuan dengan kelompok mitra berdampak pada penurunan variasi pengetahuan dan kemampuan, dimana anggota kelompok usaha wanita pengrajin purun dalam mempertahankan mutu bahan dasar dan pewarnaan sebagai peningkatan pengetahuan kelompok mitra secara merata.
Edukasi Kemasan Dan Perancangan Logo Serta Kemasan Kopi Produk Rtm Di Desa Kucur Dengan Metode Tempel Untuk Meningkatkan Brand Diversification Product
Authors: Prasetya Bintang Pramudya Putra , Nirwana Aditya , Stya Bakti Amar Ma’ruf 
Published: September 2023

RTM atau Republik Tani Mandiri merupakan salah satu organisasi kepemudaan yang bertujuan untuk meningkatkan kesejahteraan petani kopi di Desa Kucur. Desa Kucur terletak di Kabupaten Malang Kecamatan Dau dimana mayoritas masyarakat disana berprofesi sebagai petani, luas Desa Kucur tercatat seluas 58.566 hektar dengan jumlah penduduk 5690 jiwa (1575 KK) pada tahun 2017. pengetahuan untuk mengolah dan memasarkan produknya menjadi tantangan utama bagi petani kopi di Desa Kucur. Tujuan kegiatan ini adalah untuk mengimpor pengetahuan Branding, penyuluhan standar desain kemasan nasional dan pendampingan desain kemasan untuk RTM agar lebih mudah memasarkan produk kopi bagi petani di Desa Kucur. Metode yang digunakan dalam kegiatan ini adalah gabungan antara metode penelitian kualitatif dan metode desain model Design Thinking. Metode penelitian kualitatif dibagi menjadi beberapa tahapan penggalian sumber data diantaranya observasi lapangan secara langsung di Desa Kucur dan wawancara dengan pelaku usaha produk kopi di Desa Kucur yang tergabung dalam komunitas Mandiri Tani Republik (RTM). Dampak dari kegiatan yang telah dilakukan bagi mitra antara lain: 1) Mitra mampu mengidentifikasi kekurangan dan kelebihan produknya dari berbagai diskusi yang telah dilakukan, 2) Mitra memiliki pengetahuan standar pengemasan produk kopi yang lebih baik dan dapat menjaga kualitas kopi, 3) Mitra mendapatkan informasi standar informasi yang harus ada dan tertera pada label kemasan agar produknya dapat dipasarkan lebih luas, terutama jika ingin memasukkan produk ke distributor, dan 4) Perubahan besar pada kemasan membuat kepercayaan diri untuk dapat bersaing dengan produk sejenis menjadi jauh lebih meningkat.
Ukm Win’s Trophy Berkualitas Dan Berdaya Saing Untuk Meningkatkan Pendapatan Dan Perekonomian Di Malang
Authors: Putri Sri Andika , Lestari Elly , Setyawati Yuni 
Published: September 2023

Desa Bakalan Krajan merupakan salah satu sentra industri trofi di Kota Malang. Perkembangan industri kerajinan trofi mempengaruhi berbagai segi kehidupan baik dalam segi sosial maupun ekonomi. Desa Bakalan Krajan boleh dikata salah satu sentra industri kecil trofi di Kota Malang. Banyak perajin yang memproduksi trofi. Industri kecil ini terus berkembang karena produksinya mulai menembus toko-toko di Malang Raya. Salah satu industri trofi di Desa Bakalan Krajan diproduksi dengan merk Win’s Trophy yang beralamat di Perum Griya Tirta Aji Blok A-1, Sukun, Malang. Saat ini produksinya berupa trofi menghasilkan 60 sampai 120 trofi per hari dengan omzet 1,5 juta perbulan. Trofi yang dihasilkan oleh usaha mikro ini dipasarkan ke beberapa daerah di Malang Raya. Untuk memasarkan produksi tersebut pengrajin bekerja sama dengan para agen dan toko trofi di Malang Raya dengan merek Win’s Trophy. Masalah yang dihadapi mitra adalah kurangnya modal usaha, peralatan yang masih sederhana dan kapasitas produksi yang terbatas, kurangnya promosi, kurangnya sumber daya manusia dan kurangnya penerapan teknologi. Dengan permasalahan tersebut, Tim Pelaksana PKM memberikan bantuan modal usaha berupa peralatan- peralatan yang lebih modern dan lebih banyak, memberikan penyuluhan tentang tata cara berwirausaha, memberikan pendampingan tentang pemakaian peralatan yang lebih modern dan jumlah lebih banyak, pengelolaan order trofi secara profesional dan waktu yang lebih cepat.
Desain Lanskap Koridor Jalan Raya Desa Wonosari, Kecamatan Tutur, Kabupaten Pasuruan, Jawa Timur
Authors: Sitawati Sitawati , Nurlaelih Euis Elih , Suryanto Agus , Aini Nurul , Damaiyanti Dewi Ratih Rizki , Budiyono Debora 
Published: September 2023

Lanskap pedesaan yang memiliki potensi sebagai desa wisata adalah Desa Wonosari, Kecamatan Tutur, Kabupaten Pasuruan, Jawa Timur. KPSP Setia Kawan yang berada di Dusun Nongkojojor, Desa Wonosari merupakan koperasi dibidang peternakan sapi perah yang ingin mengembangkan wisata desa berbasis pertanian dan peternakan. Namun pihak KPSP Setia Kawan menyadari bahwa pengembangan wisata disertai dengan penataan lanskap desa yang indah dan fungsional. Salah satu elemen desa yang perlu diperbaiki adalah koridor jalan raya Desa Wonosari. Berdasarkan kebutuhan tersebut dibutuhkan sebuah konsep desain lanskap yang estetika dan fungsional pada koridor jalan raya Desa Wonosari. Tujuan kegiatan pengabdian adalah mendesain lanskap koridor jalan raya Desa Wonosari yang estetika dan fungsional. Metode yang digunakan dalam kegiatan pengabdian adalah Metode yang digunakan adalah metode Focussed Group Discussion (FGD). Berdasarkan hasil pengabdian kepada masyarakat yang dilakukan oleh tim pengabdi terhadap mitra Desa Wonosari memberikan manfaat yang besar dalam pengembangan desa wisata. Konsep yang diusulkan pada kawasan lanskap koridor jalan raya Desa Wonosari yaitu desain yang estetika dan fungsional dengan mempertimbangkan penggunaan elemen hard material dan soft material lokal sehingga dapat menekan biaya pemeliharaan.
Biomechanical evaluation of a novel individualized zero-profile cage for anterior cervical discectomy and fusion: a finite element analysis
Authors: Wang Yang , Liu Yang , Zhang Aobo , Han Qing , Jiao Jianhang , Chen Hao , Gong Xuqiang , Luo Wangwang , Yue Jing , Zhao Xue , Wang Jincheng , Wu Minfei 
Published: September 2023

Introduction: Anterior cervical discectomy and fusion (ACDF) is a standard procedure for treating symptomatic cervical degenerative disease. The cage and plate constructs (CPCs) are widely employed in ACDF to maintain spinal stability and to provide immediate support. However, several instrument-related complications such as dysphagia, cage subsidence, and adjacent segment degeneration have been reported in the previous literature. This study aimed to design a novel individualized zero-profile (NIZP) cage and evaluate its potential to enhance the biomechanical performance between the instrument and the cervical spine.Methods: The intact finite element models of C3-C7 were constructed and validated. A NIZP cage was designed based on the anatomical parameters of the subject’s C5/6. The ACDF procedure was simulated and the CPCs and NIZP cage were implanted separately. The range of motion (ROM), intradiscal pressure (IDP), and peak von Mises stresses of annulus fibrosus were compared between the two surgical models after ACDF under four motion conditions. Additionally, the biomechanical performance of the CPCs and NIZP cage were evaluated.Results: Compared with the intact model, the ROM of the surgical segment was significantly decreased for both surgical models under four motion conditions. Additionally, there was an increase in IDP and peak von Mises stress of annulus fibrosus in the adjacent segment. The NIZP cage had a more subtle impact on postoperative IDP and peak von Mises stress of annulus fibrosus in adjacent segments compared to CPCs. Meanwhile, the peak von Mises stresses of the NIZP cage were reduced by 90.0–120.0 MPa, and the average von Mises stresses were reduced by 12.61–17.56 MPa under different motion conditions. Regarding the fixation screws, the peak von Mises stresses in the screws of the NIZP cage increased by 10.0–40.0 MPa and the average von Mises stresses increased by 2.37–10.10 MPa.Conclusion: The NIZP cage could effectively reconstruct spinal stability in ACDF procedure by finite element study. Compared with the CPCs, the NIZP cage had better biomechanical performance, with a lower stress distribution on the cage and a more moderate effect on the adjacent segmental discs. Therefore, the NIZP cage could prevent postoperative dysphagia as well as decrease the risk of subsidence and adjacent disc degeneration following ACDF. In addition, this study could serve as a valuable reference for the development of personalized instruments.
Single-cell RNA sequencing reveals distinct tumor microenvironment of ground glass nodules and solid nodules in lung adenocarcinoma
Authors: Huang Xiaofeng , Lu Zhimeng , Jiang Xuewei , Zhang Zhe , Yan Kun , Yu Guiping 
Published: September 2023

Introduction: Lung adenocarcinoma (LUAD) is the most prevalent lung cancer. LUAD presents as ground glass nodules (GGN) and solid nodules (SN) in imaging studies. GGN is an early type of LUAD with good prognosis. However, SN exhibits a more malignant behavior than GGN, including worse pathological staging and tumor prognosis. The mechanism leading to the different malignancy levels of GGN and SN remains elusive.Methods: Three patients with GGN and three patients with SN diagnosed with early LUAD were enrolled. The tumor samples were digested to a single-cell suspension and analyzed using 10× Genomic Single-cell ribonucleic acid sequences (scRNA-seq) techniques.Results: A total of 15,902 cells were obtained and classified into nine major types. The tumor microenvironment (TME) was subsequently described in detail. ScRNA-seq revealed that ribosome-related pathways and cell adhesion played similar but distinct roles in the two groups. SN also had more active cell proliferation, enriched cell cycle regulatory pathways, and severe inflammatory responses.Conclusion: We observed changes in the cellular composition and transcriptomic profile of GGN and SN. The study improved the understanding of the underlying mechanisms of lung carcinogenesis and contributed to lung cancer prevention and treatment.
A comparative analysis of gene and protein expression in chronic and acute models of photoreceptor degeneration in adult zebrafish
Authors: Kramer Ashley C. , Carthage Justin , Berry Yasmeen , Gurdziel Katherine , Cook Tiffany A. , Thummel Ryan 
Published: September 2023

Background: Adult zebrafish are capable of photoreceptor (PR) regeneration following acute phototoxic lesion (AL). We developed a chronic low light (CLL) exposure model that more accurately reflects chronic PR degeneration observed in many human retinal diseases.Methods: Here, we characterize the morphological and transcriptomic changes associated with acute and chronic models of PR degeneration at 8 time-points over a 28-day window using immunohistochemistry and 3′mRNA-seq.Results: We first observed a differential sensitivity of rod and cone PRs to CLL. Next, we found no evidence for Müller glia (MG) gliosis or regenerative cell-cycle re-entry in the CLL model, which is in contrast to the robust gliosis and proliferative response from resident MG in the AL model. Differential responses of microglia between the models was also observed. Transcriptomic comparisons between the models revealed gene-specific networks of PR regeneration and degeneration, including genes that are activated under conditions of chronic PR stress. Finally, we showed that CLL is at least partially reversible, allowing for rod and cone outer segment outgrowth and replacement of rod cell nuclei via an apparent upregulation of the existing rod neurogenesis mechanism.Discussion: Collectively, these data provide a direct comparison of the morphological and transcriptomic PR degeneration and regeneration models in zebrafish.
Fecal microbiota transplantation inhibited neuroinflammation of traumatic brain injury in mice via regulating the gut–brain axis
Authors: Hu Xuezhen , Jin Hangqi , Yuan Shushu , Ye Tao , Chen Zhibo , Kong Yu , Liu Jiaming , Xu Kaihong , Sun Jing 
Published: September 2023

IntroductionRecent studies have highlighted the vital role of gut microbiota in traumatic brain injury (TBI). Fecal microbiota transplantation (FMT) is an effective means of regulating the microbiota–gut–brain axis, while the beneficial effect and potential mechanisms of FMT against TBI remain unclear. Here, we elucidated the anti-neuroinflammatory effect and possible mechanism of FMT against TBI in mice via regulating the microbiota–gut–brain axis.MethodsThe TBI mouse model was established by heavy object falling impact and then treated with FMT. The neurological deficits, neuropathological change, synaptic damage, microglia activation, and neuroinflammatory cytokine production were assessed, and the intestinal pathological change and gut microbiota composition were also evaluated. Moreover, the population of Treg cells in the spleen was measured.ResultsOur results showed that FMT treatment significantly alleviated neurological deficits and neuropathological changes and improved synaptic damage by increasing the levels of the synaptic plasticity-related protein such as postsynaptic density protein 95 (PSD-95) and synapsin I in the TBI mice model. Moreover, FMT could inhibit the activation of microglia and reduce the production of the inflammatory cytokine TNF-α, alleviating the inflammatory response of TBI mice. Meanwhile, FMT treatment could attenuate intestinal histopathologic changes and gut microbiota dysbiosis and increase the Treg cell population in TBI mice.ConclusionThese findings elucidated that FMT treatment effectively suppressed the TBI-induced neuroinflammation via regulating the gut microbiota–gut–brain axis, and its mechanism was involved in the regulation of peripheral immune cells, which implied a novel strategy against TBI.
The propylitic alteration in the Ponce Enriquez Gold Mining district, Azuay province, Ecuador: genetic constraints from a mineral chemistry and fluid inclusions study
Authors: Fulignati Paolo , Mulas Maurizio , Villalta Echeverria Michelle Del Pilar , Fornasaro Silvia , Larreta Erwin , Mendoza Arteaga Pierina Lisbeth , Menoscal Menoscal Melanie Annabela , Romero-Crespo Paola , Gioncada Anna 
Published: September 2023

Wide areas of the Southern sector of Ecuador are characterized by Cretaceous mafic volcanic rocks, pervasively altered by a propylitic mineralogical assemblage with epidote, chlorite, and quartz with minor titanite, illite and prehnite. These propylitically altered rocks host significant gold mineralization in veins, deeply exploited in the last decades. Porphyry Au-Cu deposits also occur in the area. This work focuses on the study of this propylitic alteration to assess the genetic conditions and the relation with the distance from the porphyry system, through the chemical composition of the secondary minerals (particularly trace elements in epidote and chlorite) and fluid inclusion data. The aim is to improve knowledge of the trace element geochemistry of secondary minerals in propylitic alteration and their relationship to the causative porphyry systems. The results of this work indicate that alteration affecting the rocks of the Pallatanga Fm. can be related to the propylitic hydrothermal alteration halo of porphyry copper deposits. Fluid inclusion investigation in quartz veins associated with epidote and chlorite evidenced at least two pulses of hydrothermal fluids characterized by temperature around 345°C for the first one and around 305°C for the second one. The salinity of the fluids is always low (around 1.5 wt% NaClequiv.) suggesting a dominantly meteoric component. Evidence of boiling processes have not been observed. We hypothesize that the hydrothermally altered “green rocks” of the Ponce Enriquez Mining District could be envisaged as the propylitic halo of the Miocene hornblende-bearing Au-Cu porphyry deposits of Gaby-Papa Grande, and discuss the epidote and chlorite trace element composition in this scenario. The results help improving the general knowledge of the propylitically altered rocks in southern Ecuador and add new data for the use of trace element chemistry of epidote and chlorite in mineral exploration elsewhere.
Association of alpha-aminoadipic acid with cardiometabolic risk factors in healthy and high-risk individuals
Authors: Desine Stacy , Gabriel Curtis L. , Smith Holly M. , Antonetti Olivia R. , Wang Chuan , Calcutt M. Wade , Doran Amanda C. , Silver Heidi J. , Nair Sangeeta , Terry James G. , Carr John Jeffrey , Linton MacRae F. , Brown Jonathan D. , Koethe John R. , Ferguson Jane F. 
Published: September 2023

IntroductionPlasma levels of the metabolite alpha-aminoadipic acid (2-AAA) have been associated with risk of type 2 diabetes (T2D) and atherosclerosis. However, little is known about the relationship of 2-AAA to other cardiometabolic risk markers in pre-disease states, or in the setting of comorbid disease.MethodsWe measured circulating 2-AAA using two methods in 1) a sample of 261 healthy individuals (2-AAA Study), and 2) in a sample of 134 persons comprising 110 individuals with treated HIV, with or without T2D, a population at high risk of metabolic disease and cardiovascular events despite suppression of circulating virus, and 24 individuals with T2D without HIV (HATIM Study). We examined associations between plasma 2-AAA and markers of cardiometabolic health within each cohort.Results and discussionWe observed differences in 2-AAA by sex and race in both cohorts, with higher levels observed in men compared with women, and in Asian compared with Black or white individuals (P&lt;0.05). There was no significant difference in 2-AAA by HIV status within individuals with T2D in the HATIM Study. We confirmed associations between 2-AAA and dyslipidemia in both cohorts, where high 2-AAA associated with low HDL cholesterol (P&lt;0.001) and high triglycerides (P&lt;0.05). As expected, within the cohort of people with HIV, 2-AAA was higher in the setting of T2D compared to pre-diabetes or normoglycemia (P&lt;0.001). 2-AAA was positively associated with body mass index (BMI) in the 2-AAA Study, and with waist circumference and measures of visceral fat volume in HATIM (all P&lt;0.05). Further, 2-AAA associated with increased liver fat in persons with HIV (P&lt;0.001). Our study confirms 2-AAA as a marker of cardiometabolic risk in both healthy individuals and those at high cardiometabolic risk, reveals relationships with adiposity and hepatic steatosis, and highlights important differences by sex and race. Further studies are warranted to establish molecular mechanisms linking 2-AAA to disease in other high-risk populations.