bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of some heavy metal pollution on idiopathic recurrent spontaneous abortion
Authors: El-Sikaily Amany , Helal Mohamed , Nsonwu-Anyanwu Augusta Chinyere , Azab Hossam , ElMoneim Neveen Abd , Mohammed Jihan Hassan , Hassanein Tamer , Abdel Aziza 
Published: February 2023

Abstract
Background
Environmental pollution with heavy metal (HM) may be associated with human reproductive failure where pollutants are accumulated in marine organisms and sediment and subsequently transferred to man through food chain. Oxidative stress and disruption of homeostatic regulation of reproductive hormones has been implicated in HM induced reproductive toxicity. The HM, indices of oxidative stress, reproductive hormone and red cell indices in women with recurrent pregnancy loss (RPL) were assessed in this study.
Methods
Seventy-six women (20–35 years) categorized into 18 fertile women without RPL (control group), and Groups I, II and III comprising 24, 18 and 16 women with RPL (2, 3, and > 3 abortions respectively) were studied. Whole blood samples were collected for the estimation of cadmium (Cd), lead (Pb), metallothionein (rbcMT), malondialdehyde (MDA), reduced glutathione (GSH), progesterone, haemoglobin (Hb), mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH). Mussel samples were collected for Cd, Pb and metallothionein (MT) estimation.
Results
The cadmium content of mussels were above the maximum limit. Women with RPL (Groups I-III) had higher Cd, Pb, rbcMT and MDA and lower catalase, GSH, P4, Hb, MCV and MCH compared to Group I (p = < 0.001). Negative associations were observed between Cd and catalase (r=-0.320, p = 0.014), GSH (r=-0.359, p=-0.006) and MCV (r=-0.391, p=-0.002) respectively and between Pb and GSH (r=-0.501, p = < 0.001), Hb (r=-0.289, p = 0.028), MCV (r=-0.394, p = 0.002) and MCH (r=-0.277, p = 0.036) respectively in women with RPL. Positive correlations were observed between Cd and Pb (r = 0.533, p = < 0.001), rbcMT with Cd (r = 0.312, p = 0.017), Pb (r = 0.488, p = < 0.000) and MDA (r = 0.282, p = 0.032) respectively in women with RPL.
Conclusion
Elevated cadmium levels in mussels, metallothionein, MDA and reduced antioxidants, progesterone and red cell indices observed in women with RPL suggest that HM induced oxidative stress and hormonal imbalance may be implicated in recurrent pregnancy loss.
Improving the accuracy of blood pressure measuring devices in Australia: a modelled return on investment study
Authors: Desson Zachary , Sharman James , Searles Andrew , Schutte Aletta , Delles Christian , Olsen Michael , Ordunez Pedro , Hure Alexis , Morton Rachael , Figtree Emma , Webster Jacqui , Jennings Garry , Redfern Julie , Nicolls Stephen , McNamara Martin , Deeming Simon , Doyle Kerry , Ramanathan Shanthi 
Published: February 2023

Abstract
Introduction:

The VALID BP project was initiated to increase the availability of validated blood pressure measuring devices (BPMDs). The goal is to eliminate non validated BPMDs and minimise over- and underdiagnosis of hypertension caused by inaccurate readings. This study was undertaken to assess the potential return on investment in the VALID BP project.

Methods:

The Framework to Assess the Impact of Translational health research was applied to the VALID BP project. One of the three methods used included a cost benefit analysis to monetise past research investment and model future research costs, implementation costs and benefits. Analysis was based on reasoned assumptions about potential impacts from availability and use of validated BPMDs (assuming an end goal of 100% validated BPMDs available in Australia by 2028) and improved skills leading to more accurate BP measurement.

Results:

After five years, with 20% attribution of benefits, there is a potential $1.29 return for every dollar spent if the proportion of validated BPMDs and staff trained in proper BP measurement technique increased from 20–60%. After eight years (2020–2028) and assuming universal validation and training coverage, the returns would be $3.20 per dollar spent (not including cost of side-effects of unnecessary medication or downstream patient impacts from unmanaged hypertension).

Conclusion:

This modelled economic analysis indicates there will be positive downstream economic benefits if the availability of validated BPMDs is increased. The findings support ongoing efforts toward a universal regulatory framework for BPMDs and can be considered within more detailed future economic analyses.
PET117 Modulates Mitochondrial-encoded COX1 Translation by stabilizing TACO1
Authors: Sun Qiong , Shi Le , Li Shuaijun , Feng Yan , Wang Shuxia , Li Jialu , Li Xinyue , Zhang Ruifen , Huang Xinghuai , Shao Yongping , Feng Zhihui , Peng Yunhua , Yang Zhiwei , Liu Jiankang , Liu Huadong , Long Jiangang 
Published: February 2023

Abstract
Mitochondria are the most important organelles in energy metabolism. Complexes of the mitochondrial oxidative phosphorylation system (OXPHOS) are formed by proteins of dual origin synthesized in the cytosol or mitochondria. The mechanisms underlying specific translation by mitoribosomes in mammals are largely unknown. Here, we report a chaperone protein of cytochrome c oxidase (COX) assembly, PET117 plays a critical role in the synthesis of mitochondrial-encoded COX1 protein in human cells. Lack of PET117 impaired mitochondrial function via suppression of mitochondrial oxygen consumption rate (OCR), probably due to reduced stability of TACO1, a COX1 translational activator. We uncovered the role of PET117 in mitochondrial regulation and a novel PET117-TACO1 axis modulating mitochondrial gene expression.
New drugs and stock market: how to predict pharma market reaction to clinical trial announcements
Authors: Budennyy Semen , Kazakov Alexey , Kovtun Elizaveta , Zhukov Leonid 
Published: February 2023

Abstract
Pharmaceutical companies operate in a strictly regulated and highly risky environment in which a single slip can lead to serious financial implications. Accordingly, the announcements of clinical trial results tend to determine the future course of events, hence being closely monitored by the public. In this work, we provide statistical evidence for the result promulgation influence on the public pharma market value. Whereas most works focus on retrospective impact analysis, the present research aims to predict the numerical values of announcement-induced changes in stock prices. For this purpose, we develop a pipeline that includes a BERT-based model for extracting sentiment polarity of announcements, a Temporal Fusion Transformer for forecasting the expected return, a graph convolution network for capturing event relationships, and gradient boosting for predicting the price change. The challenge of the problem lies in inherently different patterns of responses to positive and negative announcements, reflected in a stronger and more pronounced reaction to the negative news. Moreover, such phenomenon as the drop in stocks after the positive announcements affirms the counterintuitiveness of the price behavior. Importantly, we discover two crucial factors that should be considered while working within a predictive framework. The first factor is the drug portfolio size of the company, indicating the greater susceptibility to an announcement in the case of small drug diversification. The second one is the network effect of the events related to the same company or nosology. All findings and insights are gained on the basis of one of the biggest FDA (the Food and Drug Administration) announcement datasets, consisting of 5436 clinical trial announcements from 681 companies over the last five years.
APOBEC3B coordinates R-loop to promote replication stress and sensitize cancer cells to ATR/Chk1 inhibitors
Authors: Zong Chunyan , Zhang Zhe , Gao Li , He Jie , Wang Yiran , Li Qian , Liu Xiaoting , Yang Jie , Chen Di , Huang Rui , Zheng Guopei , Jin Xiaoliang , Wei Wu , Jia Renbing , Shen Jianfeng 
Published: February 2023

Abstract
The cytidine deaminase, Apolipoprotein B mRNA editing enzyme catalytic subunit 3B (APOBEC3B, herein termed A3B), is a critical mutation driver that induces genomic instability in cancer by catalyzing cytosine-to-thymine (C-to-T) conversion and promoting replication stress. However, the detailed function of A3B in replication stress is not fully determined and it is not known whether the mechanism of A3B action can be exploited for cancer therapy. Here, we conducted an immunoprecipitation-mass spectrometry (IP-MS) study and identified A3B to be a novel binding component of R-loops, which are RNA:DNA hybrid structures. Mechanistically, overexpression of A3B exacerbated replication stress by promoting R-loop formation and altering the distribution of R-loops in the genome. This was rescued by the R-loop gatekeeper, Ribonuclease H1 (RNASEH1). In addition, a high level of A3B conferred sensitivity to ATR/Chk1 inhibitors in melanoma cells, which was dependent on R-loop status. Together, our results provide novel insights into the mechanistic link between A3B and R-loops in the promotion of replication stress in cancer. This will inform the development of markers to predict the response of patients to ATR/Chk1 inhibitors.
Magnetic proximity-induced energy gap of topological surface states
Authors: Wang Jiashu , Wang Tianyi , Ozerov Mykhaylo , Zhang Zhan , Ortiz Joaquin Bermejo , Bac Seul-Ki , Trinh Hoai , Zhukovskyi Maksym , Orlova Tatyana , Ambaye Haile , de Vaulchier Louis-Anne , Keum Jong Kahk , Guldner Yves , Lauter Valeria , Smirnov Dmitry , Liu Xinyu , Assaf Badih 
Published: February 2023

Abstract
Topological crystalline insulator surface states can acquire an energy gap when time reversal symmetry is broken by interfacing with a magnetic insulator. Such hybrid topological-magnetic insulator structures can be used to generate novel anomalous Hall effects and to control the magnetic state of the insulator in a spintronic device. In this work, we successfully observe the energy gap of topological surface states in proximity with a magnetic insulator using magnetooptical Landau level spectroscopy. The measurements are carried out on Pb1-xSnxSe – EuSe heterostructures grown by molecular beam epitaxy exhibiting record mobility and a low Fermi energy. Through temperature dependent measurements combined with theoretical calculations, we show that the origin of this gap is likely related to quantum confinement, which allows us to conclude that the magnetic proximity effect is weak in this system. The recovered energy gap sets an upper limit for the Fermi level needed to observe the quantized anomalous Hall effect using magnetic proximity heterostructure
Signal Propagation in Resettable Mechanical Logic
Authors: Panas Robert , Sun Frederick , Farzaneh Amin , Cortes John , Bekker Logan , Johnson Hilary , Mancini Julie , Pascall Andrew , Hopkins Jonathan 
Published: February 2023

Abstract
Unconventional computing, such as mechanical1 and microfluidic logic circuits2, quantum gates3, and mechanical metamaterials4 create opportunities for embedded computation, which overcome the power5, package size, and environmental limitations of conventional electronics. Emerging micro-manufacturing capabilities6 with environmentally robust materials enable mechanical logic circuits miniaturization. Kinematically, bistable logic propagates binary signals through cascading gate displacement transitions. Energetically, the inter- and intra- node compliances are tuned for re-programmable signal propagation. Applications need computational architectures which integrate resettable signal propagation7–10, logical operation11–16, and signal storage17–19. While many researchers explore aspects of these elements1, 20–23, none consider energetic limits and propagation dynamics to evaluate and advance the field. Here, we show a generalized model and metrics, validated by experimental results, that enables the design of scale-independent, resettable, mechanical logic circuits. By studying propagation energy flows, we identified non-dimensional operating regimes in which signals propagate and resettable logic is possible. We provide deterministic design methods to evaluate future divergent topologies for displacement-based mechanical logic structures. Our results demonstrate the framework for designing densely integrated mechanical computation systems which harvest available ambient energy to propagate computational cascades. This logic responds to multi-dimensional environmental inputs and thus enables re-programmable, powerless, and embedded computation.
Cuproptosis related gene PDHB was identified as a biomarker and its up-regulation inhibited the invasion of renal clear cell carcinoma
Authors: Wang Hu , Yang Zhan , He Xingyu , Guo Fengran , Sun Hao , Xu Sen , Xu Chao , Wang Zhu , Wen Hongzhuang , Teng Zhihai , Wang Yaxuan , Han Zhenwei 
Published: February 2023

Abstract
Background
Cuproptosis is a newly discovered programmed cell death dependent on mitochondrial respiratory disorder induced by copper overload. PDHB is one of the genes responsible for cuproptosis and is a nuclear encoded pyruvate dehydrogenase, an enzyme that catalyzes the conversion of pyruvate to acetyl coenzyme A. However, the mechanism of PDHB in renal clear cell carcinoma remains unclear.
Methods
We used data from TCGA and GEO to assess the expression of PDHB in normal and tumor tissues. We analysed the relationship between PDHB and somatic mutations and immune infiltration. Finally, we explored the impact of overexpressed PDHB on renal clear cell carcinoma.
Results
PDHB is lowly expressed in tumor tissue and reduced in high-grade tumors. Highly expressed PDHB has a better prognosis in ccRCC. In ccRCC, low PDHB expression may be associated with higher VHL, PBRM1 and KDM5C mutations. Addition of copper chloride to the 786-O cell line resulted in inhibition of cell growth and increased expression of the cuproptosis genes DLAT, PDHB and FDX1. Finally, the experiments verified that overexpression of PDHB inhibited the proliferation and migration of ccRCC cells.
Conclusion
Our results demonstrate that elevated PDHB expression inhibits the proliferation, migration and invasion of renal clear cell carcinoma cells, improves the prognosis of renal cancer patients and may provide a new therapeutic strategy for patients with advanced renal cancer.
Severe clozapine toxicity-The pharmacokinetic profile of clozapine and it’s important role in hemodynamic：case report
Authors: Yu Zhu-Xi , PI Yang , Chen Meikai , Dong Danjiang , Gu Qin 
Published: February 2023

Abstract
Background: Hemodynamic instability in patients with clozapine intoxication can indirectly reflect the serum concentration of clozapine.
Case presentation: We have described a case of a 32-year-old pregnant woman who developed life-threatening clozapine toxicity at 28 weeks of gestation. The levels of clozapine and norclozapine in the serum were high.We initiated hemoperfusion(HP) and other detoxification therapies to remove the drug. The patient had severely dilated peripheral blood vessels, which led to cardiac symptoms such as fatal hypotension and uncontrollable tachycardia, resulting in very high cardiac output and elevated Central venous oxygen saturation(ScvO2). Pharmacological intervention significantly improved the hemodynamics.
Conclusions: Based on our findings in the current case, we hypothesize that hemodynamic monitoring before and after blood detoxification can help assess the efficacy and guide treatment.
Transcriptomic profiles of Pectoralis major muscles affected by spaghetti meat and woody breast in broiler chickens
Authors: Che Sunoh , Pham Phuc , Barbut Shai , Bienzle Dorothee , Susta Leonardo 
Published: February 2023

Abstract
Spaghetti meat (SM) and woody breast (WB) are breast muscle myopathies of broiler chickens, characterized by separation of myofibers and by fibrosis, respectively. This study sought to investigate the transcriptomic profiles of breast muscles affected by SM and WB. Targeted sampling was conducted on a flock to obtain 10 WB, 10 SM, and 10 Normal Pectoralis major muscle samples. Total RNA was extracted, cDNA was used for pair-end sequencing, and differentially expressed genes (DEGs) were determined by a false discovery rate of < 0.1 and a > 1.5-fold change. Principal component and heatmap cluster analyses showed that SM and WB samples clustered together. No DEGs were observed between SM and WB fillets, while a total of 4,018 and 2,323 DEGs were found when comparing SM and WB, respectively, against Normal samples. In both SM and WB samples, Gene Ontology terms associated with extracellular environment and immune response were enriched. The KEGG analysis showed enrichment of cytokine–cytokine receptor interaction and extracellular matrix–receptor interaction pathways in both myopathies. Although SM and WB are macroscopically different, the similar transcriptomic profiles suggest that these conditions may share a common pathogenesis. The similarity between the two conditions may be used to find mitigation measures.
A cluster randomized controlled trial of small quantity lipid based supplements versus unconditional cash transfer in the prevention of chronic malnutrition in Southern Angola: study protocol for the MuCCUA study.
Authors: Custodio E , Herrador Z , Trigo E , Romay-Barja M , Ramirez F , Aguado I , Iraizoz E , Silva-Gerardo A , ML Faria , Martin-Cañavate R , Marques T , Vargas A , Gómez A , Molina I 
Published: February 2023

Abstract
Background: Chronic malnutrition is a condition that can have important negative impacts on subsequent physical and cognitive development. It is multi-causal and can start very early in life, already in utero, thus it is especially challenging to find appropriate interventions to tackle it. The supplementation with small quantity lipid based nutrients (SQ-LNS) and the provision of cash transfers are promising interventions to prevent it but more operational research is needed to gain evidence on their effectiveness.
Methods/Design: The Crescer project aims to evaluate the impact of a SQ-LNS intervention and a cash transfer intervention in the lineal growth of children less than 2 years old in Southern Angola. The three-arm parallel cluster randomised controlled trial is set in four comunas of Huila and Cunene provinces. Pregnant women are recruited, and treatment arms receive unconditional cash or SQ-LNS plus a food family ration for the first 1000 days, from pregnancy through the child reaching 24 months. A comparison group receives “standard of care” provided by the Crescer project to which all groups have the same access. The primary outcome is the prevalence of stunting measured as height-for-age Z-score (HAZ) < -2 in children below 2 years. Impact will be assessed at 3, 6, 12, 18 and 24 months of children’s age. Secondary outcomes include mortality, morbidity, caring, hygiene and nutrition behaviours and practices, and women and children’s dietary diversity. Quantitative data are also collected on women’s empowerment, household food security, expenditure and relevant clinical and social events at base line, end line and intermediate time points.
Discussion: The results of this trial will provide valuable information on the impact of a SQ LNS and an unconditional cash transfer intervention, carried out during the first 1000 days, in the children´s growth up to 2 years, and related outcomes.
Trial registration: Clinical Trials NCT05571280. Registered 7 October 2022.
Reporting quality of randomized controlled trials of angina pectoris with integrated traditional Chinese and western medicine interventions: a cross-sectional study
Authors: Deng Jiashuai , He Juan , Wang Juan , Cheng Chung Wah , Jiao Yalin , Wang Nana , Li Ji , Wang Ping , Han Fei , Lyu Aiping , Bian Zhaoxiang , Zhang Xuan 
Published: February 2023

Abstract
Background and objective: Integrated traditional Chinese and western medicine (ITCWM), as a representative type of complex intervention, is commonly used for the treatment of angina pectoris (AP) in clinical practice. However, it is unclear whether the details of ITCWM interventions, such as selection rationale, implementation, study design and potential interactions, were adequately reported. Therefore, this study aimed to assess the quality of reporting in the ITCWM interventional randomized controlled trials (RCTs).
Methods: Through a search of 7 international electronic databases, we identified RCTs of AP with ITCWM interventions published in both English and Chinese from 1st Jan 2017 to 6th Aug 2022. The general characteristics of included studies were summarized, further, the quality of reporting was assessed based on three Checklists, including the CONSORT (Consolidated Standards of Reporting Trials) with 36 items (except for one item 1b about abstract), the CONSORT for abstracts (17 items), and a self-designed ITCWM-related checklist (21 items covering design rationale, intervention details, outcome assessment and analysis). The quality of RCTs published in English journals, Chinese journals and dissertations were also compared.
Results: A total of 451 eligible RCTs were included. For the reporting compliance, the mean score (standard deviation) of the CONSORT (72 score in total), CONSORT for abstract (34 score in total), and ITCWM-related (42 score in total) checklists was 35.67 (4.02), 13.17 (2.14), and 20.93 (3.95), respectively. More than 50% items were evaluated as poor quality (reporting rate＜50%) among each Checklist. Moreover, the reporting quality of publications in English journals was significantly higher than that in Chinese journals regarding the CONSORT compliance (p<0.05), but there were no differences among journals regarding the CONSORT for Abstract and ITCWM-related reporting (p>0.05). The reporting of dissertations was better than journal publications in comparison of the CONSORT and ITCWM-related items (p<0.05).
Conclusion: Although CONSORT appears to have enhanced the reporting of RCTs in AP, the quality of ITCWM specifics is variable and in need of improvement. Reporting guideline of the ITCWM recommendations should be developed thus to improve their quality.
A systematic review and net meta-analysis of the effects of different warm-up methods on the acute effects of lower limb explosive strength
Authors: LI Fuyou , GUO Chenggen , LI Huashuai , XU Haoran , SUN Pu 
Published: February 2023

Abstract
Objective: To evaluate the effects of different warm-up methods on the acute effect of lower limb explosive strength with the help of a reticulated meta-analysis system and to track the optimal method.
Methods: R software combined with Stata software, version 13.0, was used to analyse the outcome metrics of the 35 included papers.
Results: 1) Static combined with dynamic stretching [MD = 1.80, 95% CI: (0.43, 3.20)] and dynamic stretching [MD = 1.60, 95% CI: (0.67, 2.60)] were significantly better than controls in terms of improving reverse long jump performance, and the effect of dynamic stretching was influenced by the duration of stretching (I2 = 80.4%), study population (I2 = 77.2%) and age (I2 = 75.6%) as moderating variables, with the most significant effect size for dynamic stretching time of 7-10 min. 2) Only dynamic stretching [MD = -0.08, 95% CI: (-0.15, -0.008)] was significantly better than the control group in terms of improving sprint performance, while static stretching [MD = 0.07, 95% CI: (0.002, 0.13)] showed a significant, negative effect. 3) No results were available to demonstrate a significant difference between other methods, such as foam axis rolling, and the control group.
Conclusion: Static stretching was able to reduce explosive performance, while the 2 warm-up methods, namely dynamic stretching and static combined with dynamic stretching, were able to significantly improve explosive performance, with dynamic stretching being the most stable and moderated by multiple variables and dynamic stretching for 7-10 min producing the best explosive performance. In the future, high-quality studies should be added based on strict adherence to test specifications.