bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of postmortem pH on the physical, biochemical, and sensory characteristics of bovine longissimus thoracis et lumborum muscle
Authors: Khatri Yunus , Huff‐Lonergan Elisabeth 
Published: January 2023

AbstractFrom a large feeding trial study consisting of 299 bulls and steers, 15 carcasses exhibited stress‐related syndromes manifested by atypical color and pH which were then selected for subsequent analysis. Samples of longissimus thoracis et lumborum muscle with postmortem pH in the range of 5.5–6.9 were subjected to a 14‐day aging period at 2°C. Sensory panel tenderness, connective tissue, juiciness, and flavor intensity of high pH (6.4–6.9) meat were significantly different (p < .05) from samples of intermediate pH (6.0–6.1) as well as normal pH (5.5). Muscles at pH 6.0–6.1 were the toughest samples. This was confirmed by Warner–Bratzler shear force (WBSF), residual force, and myofibril fragmentation index. Palatability attributes of normal pH (5.5) samples were significantly different (p < .05) from dark‐cutting beef in terms of tenderness and flavor and at the high pH extreme. The increase in WBSF at pH 6.0–6.1, lack of extensive degradation of muscle proteins, and the decreased sarcomere length resulted in tougher meat than low or high pH muscles. Sodium dodecyl‐sulfate polyacrylamide gel electrophoresis of meat at the high pH extreme (6.7–6.9) revealed that the breakdown of troponin‐T to 30 kD was complete while at intermediate pH (6.0–6.1) was incomplete. In addition, the appearance of a ‘doublet’ on high‐molecular‐weight resolution gels may also account for the greater tenderness experienced by sensory panelists.
P–N Heterojunction System Eu‐Doped ZnO@GO for Photocatalytic Water Splitting
Authors: Gurbani Neeta , Chouhan Neelu 
Published: January 2023

AbstractHere, a feather‐like Eu‐doped ZnO (particle size ≈ 34.87 µm and Eg ≈ 3.13 eV) nanoassembly is synthesized by using the capping agent cetyltrimethylammonium bromide‐supported hydrothermal method. The Eu‐doped ZnO is loaded onto the graphene oxide (GO) surface as Eu‐doped ZnO@GO (particle size ≈ 23.07 µm and Eg ≈ 0.79 eV) and applied to measure the photocatalytic water splitting activity in 20% CH3OH under a 300 W Xe light source. Eu‐doped ZnO@GO exhibits the higher hydrogen generation activity of 255.8 µmol h−1 g−1 that is 159 and 1.5 times more than the pristine GO and Eu‐doped ZnO systems, respectively. Eu‐doped ZnO enhances the photocatalytic activity of GO because the p–n junction formed between GO and Eu‐doped ZnO might support the charge‐transfer and suppress charge recombination. The light harvesting power of Eu‐doped ZnO@GO makes the charge transfer smooth through the GO network. Surface photovoltage and electrochemical impedance studies of the Eu‐doped ZnO@GO composite, reveal that GO acts as the p‐type semiconductor and Eu‐doped ZnO works as an n‐type semiconductor and their interface facilitates the p–n junction to ease charge separation and results in enhanced the water‐splitting efficiency.
Posterior hypothalamic theta rhythm: Electrophysiological basis and involvement of glutamatergic receptors
Authors: Kowalczyk Tomasz , Staszelis Agata , Bocian Renata , Siwiec Marcin , Sowa Joanna E. , Tokarski Krzysztof , Kaźmierska‐Grębowska Paulina , Caban Bartosz 
Published: January 2023

AbstractThe posterior hypothalamic area (PHa), including the supramammillary nucleus (SuM) and posterior hypothalamic nuclei, forms a crucial part of the ascending brainstem hippocampal synchronizing pathway, that is involved in the frequency programming and modulation of rhythmic theta activity generated in limbic structures. Recent investigations show that in addition to being a modulator of limbic theta activity, the PHa is capable of producing well‐synchronized local theta field potentials by itself. The purpose of this study was to examine the ability of the PHa to generate theta field potentials and accompanying cell discharges in response to glutamatergic stimulation under both in vitro and in vivo conditions. The second objective was to examine the electrophysiological properties of neurons located in the SuM and posterior hypothalamic nuclei. Extracellular in vivo and in vitro as well as intracellular in vitro experiments revealed that glutamatergic stimulation of PHa with kainic acid induces well‐synchronized local theta field oscillations in both the supramammillary and posterior hypothalamic nuclei. Furthermore, the glutamatergic PHa theta rhythm recorded extracellularly was accompanied by the activity of specific subtypes of theta‐related neurons. We identify, for the first time, a subpopulation of supramammillary and posterior hypothalamic neurons that express clear subthreshold membrane potential oscillations in the theta frequency range.
Population Pharmacokinetics as a Tool to Reevaluate the Complex Disposition of Ethanol in the Fed and Fasted States
Authors: Büsker Sören , Jones Alan Wayne , Hahn Robert G. , Taubert Max , Klotz Ulrich , Schwab Matthias , Fuhr Uwe 
Published: January 2023

AbstractThe pharmacokinetics (PK) of ethanol are important in pharmacology and therapeutics because of potential drug‐alcohol interactions as well as in forensic science when alcohol‐related crimes are investigated. The PK of ethanol have been extensively studied since the 1930s, although some issues remain unresolved, such as the significance of first‐pass metabolism, whether zero‐order kinetics apply, and the effects of food on bioavailability. We took advantage of nonlinear mixed‐effects modeling to describe blood‐alcohol concentration (BAC) profiles derived from 3 published clinical studies involving oral, intraduodenal, and intravenous administration of ethanol with and without food. The overall data set included 1510 BACs derived from 72 healthy subjects (60 men, 12 women) aged between 20 and 60 years. Two‐compartment models with first‐order absorption and Michaelis‐Menten elimination kinetics adequately described the BAC profiles. Food intake had 2 separate effects: It reduced the absorption rate constant and accelerated the maximum elimination rate. Estimates of the maximum elimination rate (fasted) and the food effect (as a factor) were 6.31 g/h (95%CI, 6.04‐6.59 g/h) and 1.39‐fold (95%CI, 1.33‐1.46‐fold), respectively. Simulations showed that the area under the BAC‐time curve (AUC) was smaller with lower input rate of ethanol, irrespective of any first‐pass metabolism. The AUC from time 0 to 10 hours for a 75‐kg subject was 2.34 g • h/L (fed) and 3.83 g • h/L (fasted) after an oral dose of 45 g ethanol. This difference was mainly attributable to the food effect on ethanol elimination and depended less on the absorption rate. Our new approach to explain the complex human PK of ethanol may help when BAC predictions are made in clinical pharmacology and forensic medicine.
Severe neonatal onset neuroregression with paroxysmal dystonia and apnoea: Expanding the phenotypic and genotypic spectrum of CARS2‐related mitochondrial disease
Authors: Poquérusse Jessie , Nolan Melinda , Thorburn David R. , Van Hove Johan L. K. , Friederich Marisa W. , Love Donald R. , Taylor Juliet , Powell Christopher A. , Minczuk Michal , Snell Russell G. , Lehnert Klaus , Glamuzina Emma , Jacobsen Jessie C. 
Published: January 2023

AbstractDisorders of mitochondrial function are a collectively common group of genetic diseases in which deficits in core mitochondrial translation machinery, including aminoacyl tRNA synthetases, are key players. Biallelic variants in the CARS2 gene (NM_024537.4), which encodes the mitochondrial aminoacyl‐tRNA synthetase for cysteine (CARS2, mt‐aaRScys; MIM*612800), result in childhood onset epileptic encephalopathy and complex movement disorder with combined oxidative phosphorylation deficiency (MIM#616672). Prior to this report, eight unique pathogenic variants in the CARS2 gene had been reported in seven individuals. Here, we describe a male who presented in the third week of life with apnoea. He rapidly deteriorated with paroxysmal dystonic crises and apnoea resulting in death at 16 weeks. He had no evidence of seizure activity or multisystem disease and had normal brain imaging. Skeletal muscle biopsy revealed a combined disorder of oxidative phosphorylation. Whole‐exome sequencing identified biallelic variants in the CARS2 gene: one novel (c.1478T>C, p.Phe493Ser), and one previously reported (c.655G>A, p.Ala219Thr; rs727505361). Northern blot analysis of RNA isolated from the patient's fibroblasts confirmed a clear defect in aminoacylation of the mitochondrial tRNA for cysteine (mt‐tRNACys). To our knowledge, this is the earliest reported case of CARS2 deficiency with severe, early onset dystonia and apnoea, without epilepsy.
SMART: A data reporting standard for mass spectrometry imaging
Authors: Xi Ying , Sohn Alexandria L. , Joignant Alena N. , Cologna Stephanie M. , Prentice Boone M. , Muddiman David C. 
Published: January 2023

AbstractMass spectrometry imaging (MSI) is an important analytical technique that simultaneously reports the spatial location and abundance of detected ions in biological, chemical, clinical, and pharmaceutical studies. As MSI grows in popularity, it has become evident that data reporting varies among different research groups and between techniques. The lack of consistency in data reporting inherently creates additional challenges in comparing intra‐ and inter‐laboratory MSI data. In this tutorial, we propose a unified data reporting system, SMART, based on the common features shared between techniques. While there are limitations to any reporting system, SMART was decided upon after significant discussion to more easily understand and benchmark MSI data. SMART is not intended to be comprehensive but rather capture essential baseline information for a given MSI study; this could be within a study (e.g., effect of spot size on the measured ion signals) or between two studies (e.g., different MSI platform technologies applied to the same tissue type). This tutorial does not attempt to address the confidence with which annotations are made nor does it deny the importance of other parameters that are not included in the current SMART format. Ultimately, the goal of this tutorial is to discuss the necessity of establishing a uniform reporting system to communicate MSI data in publications and presentations in a simple format to readily interpret the parameters and baseline outcomes of the data.
Proarrhythmic effect of bipolar epicardial left ventricular stimulation in CRT resolved maintaining biventricular pacing with unipolar‐cathodical configuration: A peculiar case report
Authors: Magnano Massimo , Devecchi Chiara , Oriente Domenico , Occhetta Eraldo , Rametta Francesco 
Published: January 2023

AbstractBackgroundThe effect of cardiac resynchronization therapy (CRT) on the risk of ventricular arrhythmias is controversial. Several studies reported a decreased risk, but some studies reported a potential proarrhythmic effect of epicardial left ventricular pacing resolved upon discontinuation of biventricular pacing (BiVp).Case SummaryA 67‐year‐old woman with a history of heart failure due to nonischemic cardiomyopathy and left bundle branch block was hospitalized for CRT device implantation. Unpredictably, as soon as the leads have been connected to the generator, an electrical storm (ES) occurred with relapsing self‐resolving polymorphic ventricular tachycardia (PVT) triggered by ventricular extra beats with short‐long‐short sequences. The ES was resolved without interrupting BiVp switching to unipolar left ventricular (LV) pacing. This allowed to keep CRT active with extreme clinical benefit for the patient and to demonstrate that the cause of the PVT was the anodic capture of bipolar LV stimulation. Reverse electrical remodeling was also demonstrated after 3 months of effective BiVp.DiscussionProarrhythmic effect of CRT is a rare but significant complication of CRT, and it may compel to discontinuation of the BiVp. The reversal of the physiological transmural activation sequence of epicardial LV pacing and subsequent prolonging of corrected QT interval have been speculated as the most probable explanation, but our case highlights the possibility that the anodic capture may play a relevant role in PVT genesis.
MGFFCNN: Two‐dimensional matrix spectroscopy combined with multi‐channel gradient feature fusion convolutional neural network means to diagnose glioma and esophageal cancer patients
Authors: Chen Chen , Meng Chunzhi , Ma Yuhua , Zhu Min , Wang Xiaohui , Xie Xiaodong , Chen Cheng 
Published: January 2023

AbstractCurrently, glioma and esophageal cancer are common malignant tumors worldwide with low cure rate and high mortality rate, and they endanger human health seriously. In this study, we analyzed the correlation and difference between glioma and esophageal cancer through serum mid‐infrared and Raman spectra and established a multi‐channel gradient feature fusion convolutional neural network to achieve rapid diagnosis of glioma and esophageal cancer patients. We transformed the spectra from one‐dimensional matrix to two‐dimensional matrix form separately as the input of the network and fused the features extracted from the Flatten layer of the network. First, we fused the features of mid‐infrared and Raman spectra and constructed a two‐channel gradient feature fusion idea. Then, in order to enrich the learning of features further, we took the first‐order derivative of mid‐infrared and Raman original spectra, respectively, and used the derivative spectra as two channels as well. The mid‐infrared and Raman spectra in two‐dimensional matrix form were fused with their derivative spectral features, respectively, and the fused features were fused again to construct a four‐channel gradient feature fusion network model. Finally, compared with the single original spectrum and the one‐dimensional matrix feature fusion spectrum, the two‐dimensional matrix feature fused spectrum was more advantageous, and the classification accuracy of the model was as high as 99.2% ± 0.7%. This study showed that two‐dimensional matrix spectra combined with multi‐channel gradient feature fusion technique had great potential for rapid and accurate identification of patients with glioma and esophageal cancer.
Edible coating enriched with cinnamon oil reduces the oxidative stress and improves the quality of strawberry fruit stored at room temperature
Authors: Piechowiak Tomasz , Skóra Bartosz 
Published: January 2023

AbstractBACKGROUNDThe present study aimed to assess the impact of a starch/gelatine coating containing cinnamon oil on selected quality attributes and redox status in strawberry fruit stored at room temperature (72 h).RESULTSResearch showed that the application of cinnamon oil to an edible coating allows an improvement of the quality of strawberry fruit stored at room temperature. The cinnamon oil coating inhibits the development of yeast and mould, and reduces loss of soluble solids and ascorbic acid during 72 h storage at room temperature. Moreover, the coating with cinnamon oil clearly reduced the level of oxidative stress, which was manifested by a lower level of reactive oxygen species, as well as a lower activity of antioxidant enzymes. The elimination of oxidative stress in the cinnamon oil‐coated fruit also contributed to lowerPARP1mRNA expression, inhibiting the metabolism of NAD+and reducing ATP losses.CONCLUSIONThe coating of strawberry fruit with a starch/gelatine biofilm containing cinnamon oil is an effective method for delaying postharvest senescence of fruit and the storage degradation of tissue. © 2023 Society of Chemical Industry.
Myer‐Cotton Grade of Subglottic Stenosis Depends on Style of Endotracheal Tube Used
Authors: Propst Evan Jon , Wolter Nikolaus Ernst 
Published: January 2023

ObjectivesDetermine percentage of subglottic stenosis using current endotracheal tube (ETT) cross‐sectional areas as actual, compared with previously published ETT cross‐sectional areas as expected, and determine if style of ETT could result in a change in percentage of stenosis or Myer‐Cotton grade.Study TypeCross‐sectional study.DesignProspective analysis.MethodsEight styles of uncuffed pediatric ETT from four manufacturers ranging from 2.0 to 6.0 inner diameter (ID) were evaluated. ID and outer diameter (OD) measurements were obtained from each company's specification sheets. Cross‐sectional area was calculated for each ETT using the formula (Area = πr2). The cross‐sectional areas of each current ETT (actual) were compared with those of previously published ETTs (expected) based on age, and the degree of stenosis was calculated using the formula [1‐ (Area actual/Area expected)] × 100%. Ranges of percentage for each style of ETT were calculated.ResultsThere was an increase in range of OD and area with increasing size of ETT ID, with the largest range in OD being 0.8 mm, and the largest range in area being 10.55 mm2. The median interquartile range (IQR), range of percentage stenoses was 11 (5%), ranging from 0% to 21%. Seven of 28 (25%) ranges were found to span two Myer‐Cotton grades.ConclusionsThe Myer‐Cotton grade of subglottic stenosis depends on the style of ETT used. Using updated values from currently available ETTs aims to keep this grading system valid with respect to surgical approach and outcomes following surgery.Level of EvidenceNA Laryngoscope, 133:2808–2812, 2023
Composition Regulation by Flow Copolymerization of Methyl Methacrylate and Glycidyl Methacrylate with Free Radical Method
Authors: Wakiuchi Araki , Takasuka Shogo , Asano Shigehito , Hashizume Ryo , Nag Aniruddha , Hatanaka Miho , Miyao Tomoyuki , Ohnishi Yuya , Matsubara Takamitsu , Ando Tsuyoshi , Sugawara Tetsunori , Fujii Mikiya , Ajiro Hiroharu 
Published: January 2023

AbstractIt is important to match the feeding ratio of comonomers to the composition ratio in the resulting copolymers as closely as possible in industrial production, where the goal is often to produce more a homogeneous composition in copolymers. In this study, a flow copolymerization system with a conventionally initiated free radical method, together with randomly selected polymerization conditions is investigated. It is succeeded in achieving a closer match between the composition ratio and feeding ratio than previously reported in the copolymerization of styrene with methyl methacrylate and of glycidyl methacrylate with methyl methacrylate, which will widen the range of applications, by precisely controlling the mixing and heating in a flow polymerization apparatus. This is confirmed by the fact that the estimated values of reactivity ratios, r1 and r2, which are used in the reaction kinetics of copolymerization, are close to 1.
The two‐dimensional stationary Navier–Stokes equations in toroidal Besov spaces
Authors: Tsurumi Hiroyuki 
Published: January 2023

AbstractWe consider the stationary Navier–Stokes equations in the two‐dimensional torus . For any , we show the existence, uniqueness, and continuous dependence of solutions in homogeneous toroidal Besov spaces for given small external forces in when . These spaces become closer to the scaling invariant ones if the difference ε becomes smaller. This well‐posedness is proved by using the embedding property and the para‐product estimate in homogeneous Besov spaces. In addition, for the case , we can show the ill‐posedness, even in the scaling invariant spaces. Actually in such cases of p and q, we can prove that ill‐posedness by showing the discontinuity of a certain solution map from to .
On the Neumann problem for the nonstationary Stokes system in angles and cones
Authors: Kozlov Vladimir , Rossmann Jürgen 
Published: January 2023

AbstractThe authors consider the Neumann problem for the nonstationary Stokes system in a two‐dimensional angle or a three‐dimensional cone. They obtain existence and uniqueness results for solutions in weighted Sobolev spaces and prove a regularity assertion for the solutions.
Insulin‐like growth factor‐2 mRNA‐binding protein 3 promotes cell migration, invasion, and epithelial−mesenchymal transition of esophageal squamous cell carcinoma cells by targeting zinc finger E‐box‐binding homeobox 1 mRNA
Authors: Feng Yadong , Lin Yanbing , Jiang Zhaoyan , Wu Lei , Zhang Youyu , Wu Hailu , Yuan Xiaoqin 
Published: January 2023

AbstractThe role and mechanism of insulin‐like growth factor‐2 mRNA‐binding protein 3 (IGF2BP3) in the metastasis of esophageal squamous cell carcinoma (ESCC) remain unclear. In this study, IGF2BP3 mRNA and protein expression levels were evaluated in ESCC tissues. Small interfering RNAs (siRNAs), plasmid overexpression, and stable lentivirus transfection were used to manipulate intracellular IGF2BP3 expression levels. The role of IGF2BP3 in ESCC tumorigenesis was investigated in vitro and in vivo. IGF2BP3 target transcripts were detected, and the acetylation effect ratios of the IGF2BP3 promoter region by H3K27ac were determined. IGF2BP3 mRNA expression levels were significantly higher in ESCC tissues than in normal esophageal tissues. Increased IGF2BP3 expression levels were detected in node‐negative ESCC tissues and correlated with greater lesion depth in ESCC. Overexpression of IGF2BP3 promoted ESCC development in vitro and in vivo, and IGF2BP3 knockdown caused an opposite effect. IGF2BP3 was found to directly bind to the zinc finger E‐box‐binding homeobox 1 (Zeb1) mRNA, and the downregulation of IGF2BP3 reduced the stability of Zeb1 mRNA. IGF2BP3 induced epithelial−mesenchymal transition in ESCC cells in a Zeb1‐dependent manner. IGF2BP3 was transcriptionally activated in ESCC cell lines via H3K27 acetylation. Our results demonstrate that IGF2BP3 plays a vital role in ESCC cell proliferation, invasion, and metastasis and is a potential therapeutic target for treating ESCC.
Optimal replenishment and transshipment management with two locations
Authors: Xu Jianjun , Keblis Matthew F. , Feng Youyi , Zhou Sean X. 
Published: January 2023

AbstractWe study the problem of optimally managing an inventory system with backorders over a finite time horizon where the objective is minimization of expected total discounted costs. The system consists of two locations each stocking the same product. At the beginning of each time period decisions are made about replenishment at each location and about any quantity to transship between the locations before demand is observed. Leveraging the L♮‐convexity of the problem's cost function; we characterize the optimal replenishment and transshipment policy for this system. More specifically, we show the optimal policy can be described using switching curves monotone in the system state. We also discuss two extensions. For a lost‐sales model, we establish L♮‐convexity and apply it to characterize the optimal policy, simplifying the analysis found in previous work. In the other extension, we investigate the optimal policy for a partial transshipment problem where only one location orders from the external supply source and then transships to the other location.