bluuebunny/crossref_metadata_2025_split huggingface dataset:
CONTROL OF CO2 LASER POWER BY ACOUSTIC FIELDS
Authors: Abrahamyan A.S. , Chilingaryan R.Yu. 
Published: March 2023

The present study investigates the optimization of the operation of the CO2 laser in the acoustoplasma mode (i.e., dependence of the laser radiation power on the composition of the working mixture, pressure, value of the direct component of the discharge current, frequency, and modulation depth). A three-dimensional dependence on the frequency and modulation depth of the discharge current is experimentally obtained for the normalized efficiency of the conversion of the electric power supplied to the discharge tube into laser power. The maximum gain when transition to the acoustoplasma mode exceeds 2.5 times. The optimum depth of the discharge current modulation is 0.5–0.7. The laser radiation power modulation caused by the discharge current modulation is measured. Laser power is not modulated at modulation frequencies of current >1 kHz. Meanwhile, at current modulation frequencies <0.5 kHz, the modulation depth of the laser radiation power nonlinearly depends on the modulation depth of the discharge current and has a threshold character. The modulation depth of the laser radiation power is associated with the creation of an acoustoplasma and not simply with the discharge current modulation.
COMPARISON OF GLOW, ARC, AND MAGNETRON DIRECT CURRENT DISCHARGES
Authors: Abrahamyan A.S. , Mkrtchyan A.H. , Chilingaryan R.Yu. 
Published: March 2023

Glow discharge (GD) in a tube, arc discharge (AD) without cathode heating from an external source, and magnetron discharge (MD) in a planar magnetron are compared. In each of the discharges, characteristic areas are distinguished. In MD, electrons trapped in the near-cathode region are not directly involved in ionization processes, but “wake” acceleration of slow ions by electrons that move along Larmor orbits is possible, which gives additional energy to ions moving toward the cathode. In GD in the near-cathode region, the average energy of the ejected electrons is on the order of several electron volts, and the energy of the ions and neutrals is less than 0.1 eV. In MD in the near-cathode region, the average energy of knocked-out electrons, ions, and neutrals is on the order of tens of electron volts. The differential resistance of GD is negative, that of AD is usually negative, and that of MD is positive. The energy of ions in the magnetron plasma can be greater than that of electrons, which gives new possibilities for acoustoplasma control of MD and the creation of appropriate instruments and devices.
Diagnostic and Therapeutic Approaches for Endometriosis: A Patent Landscape
Authors: Singh Maninder , Jassal Reena , Khetarpal Preeti 
Published: March 2023

Abstract
Objective

The aim of this review is to analyse the patent filings and to systematize the main technological trends in patent protection for the diagnosis and therapeutics for Endometriosis. Patent literature has also been explored to find out active inventors and applicants in this field.

Methodology

Patent Search is carried in the freely accessible Patent search databases namely Patentscope using Various combinations of the key words “Endometriosis OR Adenomyosis” AND “Diagnostic OR Therapeutics”, were used along with wildcard search query in the “Title”, “Abstract” and “Descriptions” fields.

Results

Patent search revealed one hundred forty-four patents which describes invention for endometriosis diagnostic and therapeutic purposes. These patents includes twenty six patent applications in diagnostic utility, one hundred sixteen patents applications under the therapeutic approaches and forty three patent applications describes under traditional medicines for endometriosis.

Conclusion

Hormonal alteration is the most active field of invention followed by surgical interventions. Although there is a general trend of increase in patent applications has been observed but there is slight decrease in the recent years has also been observed.
The effect of manipulating glucuronic acid biosynthetic pathway in Bacillus subtilis strain on hyaluronic acid production
Authors: Afrasiabi Shadi , Zanjani Fatemeh Sadat Amjad , Ahmadian Gholamreza , Cohan Reza Ahangari , keramati malihe 
Published: March 2023

Abstract
Hyaluronic acid (HA), composed of glucuronic acid (GlcUA) and N-acetyl glucoseamine (GlcNAc), is a versatile biopolymer with high commercial value and innumerous physiological roles and pharmaceutical applications. The hasA gene is responsible for HA biosynthesis by Streptococcusstrain as a natural producer. The hasB and hasC genes are also responsible for GlcUA precursor biosynthesis. In the present study, S. equisimilis hasA gene; B. subtilis tuaD and gtaB genes for GlcUA precursorsenhancement, and vgb gene coding bacterial hemoglobin as an oxygen provider were used to engineer the B. subtilis strainfor HA production. RBSHA (hasA), RBSHA2 (hasA/tuaD/gtaB), and RBSHA3 (hasA/tuaD/gtaB/vgb) strains were developed and confirmed through genotype and phenotype analysis. After HA production and purification, FTIR spectroscopy confirmed the HA structures produced by the strains. HA assay showed the highest HA titer for RBSHA3 (2.1 ± 0.18 mg/ml) and then RBSHA2 (1.9 ± 0.03 mg/ml), and RBSHA (0.6 ± 0.14 mg/ml). Statistical analysis indicated there is no significant difference in HA titer between RBSHA2 and RBSHA3 strains (p-value > 0.05), however, these strains produced HA approximately 4-fold higher than that of RBSHA strain. Agarose gel electrophoresis showed the same molecular weight (< 30 kDa) of produced HA. Dynamic light scattering (DLS) revealed all HA polymers had a relatively low polydispersity index (PDI < 0.5). Our study demonstrates the successful GlcUA biosynthetic pathway engineering strategy in improving HA yield by recombinant B.subtilis, metabolically-robust, and industrially potential strain.
To assess the impact of problem lists' accuracy in electronic health records on clinical decision-making: protocol for a randomized controlled trial
Authors: Klappe Eva Susanne , Heijmans Jarom , Groen Kaz , Cornet Ronald , de Keizer Nicolette F 
Published: March 2023

Abstract
Background Problem-oriented electronic health record (EHR) systems, with complete, coded and up-to-date (i.e. accurate) problem lists, aid healthcare providers to track patient’s health status and make better decisions, as problem lists can provide useful summaries of important health issues. Unfortunately, problem lists are still incomplete and out-of-date (i.e. inaccurate). That is, among others, because providers are under time pressure, prefer writing free-text notes and are often unwilling to update problem lists, unless receiving returns for their effort. This study aims to assess the impact of accuracy of problem lists in EHRs on clinical decision-making.

Methods In a laboratory setting we will perform a crossover randomized controlled trial in which we will recruit individual Dutch healthcare providers on-site at Amsterdam University Medical Centers. Participants will be presented with two records of two patients (A and B): one with an accurate and one with an inaccurate problem list, created in a training environment of the EPIC EHR in agreement with clinical experts. Randomization determines which record will have the accurate problem list. Participants are informed that EHR-usage is investigated and do not know which record has an accurate or inaccurate problem list. Participants will provide a motivated (Yes/No) answer on whether prescribing medication X and medication Y is appropriate. Medication Y cannot be prescribed in both patient records, due to contraindicated diagnosis (A) and diagnosis-related medical history (B). Medication X serves as a control question, and is contraindicated based on an allergy, which is equally documented in the records. The primary outcome measure is the correctness of the motivation for the correct answer of medication Y. Secondary outcome measures are: correctness of X (and Y) with the right motivation(s); total time to answer X and Y where the motivation for Y (and X) is correct. Time stamps are registered between opening the question and confirming two Yes/No answers. Proportion of retrieved correct answers and time to answer(s) retrieval will be compared by Chi-square, McNemar tests and Log Rank survival analyses. Alternative analytical models will be applied if necessary.

Discussion If accurate problem lists lead to faster and better decision-making resulting in better patient outcomes, one may be motivated to develop future policies for this area and healthcare providers may be persuaded to use and update problem lists, also leading to improved data quality and opportunities for reuse.
Plastic mulching reduces surface-soil microbial biomass carbon and structural stability in a pear orchard
Authors: Wu Yang , Zhao Zhi , Sun Mingde , Liu Songzhong 
Published: March 2023

Abstract
Background and aims Plastic mulching (PM) can improve soil aggregate stability and microbial activity, thereby preventing soil degradation in crop fields, which are different from the conditions in the orchard. However, little is known about the response of surface-soil microbial and structural stability to PM applied in orchards. Methods An experiment was conducted from 2017 to 2020 in a pear orchard. Two treatments consisted of no mulching and plastic (black woven polypropylene fabric) lying under a pear tree canopy. Results Results showed that the soil microbial biomass carbon content (MBC), organic carbon content (SOC), and ratio of MBC/SOC of PM treatment declined by 49.2%, 13.4%, and 41.3%, respectively. In particular, the significant decline in ratio of MBC/SOC indicated that SOC content would decrease in the future. Mean weight diameter and geometric diameter of soil aggregates in PM treatment decreased by approximately 20%, as compared with no mulching. The decrease in MBC, SOC and soil aggregates stability under PM were mainly resulted from the reduction in root exudate production and root-associated symbionts, causing by the absence of grass roots. These can be proved by the soil gas exchange and concentration. Soil respiration and mineralization were decreased by PM, but soil oxygen concentration at 10 cm depth in PM were improved, due to the oxygen consumed by microbial in no mulching treatment and low gas exchange at this depth. Conclusions Unlike the crop field, protecting the surface soil in orchard from degradation under long-term plastic mulching application is worth considering.
Dual trigger for final oocyte maturation in expected normal responders with a high immature oocyte rate: A randomized controlled trial
Authors: Yan Meng-Han , Zhang Qian-Qian , Yang Wen-Xiu , Sun Zhen-Gao , Song Jing-Yan 
Published: March 2023

Abstract
Purpose
To evaluate whether dual trigger could improve reproductive outcomes in women with low oocyte maturation rates compare to human chorionic gonadotropin (hCG) trigger.
Methods
This study included expected normal ovarian responders younger than 40 years old whose immature oocyte rate in the previous cycle was more than 50% at the reproductive center from July 2021 to January 2023. A total of 73 patients were enrolled at trigger, including 34 in the hCG trigger group and 39 in the dual trigger group (co-administration of gonadotrophin releasing hormone (GnRH) agonist and hCG, 40 and 34 hours prior to oocyte retrieval, respectively). The primary outcome was oocyte maturation rate.
Results
There was no significant difference in the number of oocytes retrieved between the two study groups, but the oocyte maturation rate was higher in dual trigger group (84.0% [14.0%] vs. 55.5% [19.8%], P < 0.001). Moreover, there were also higher cumulative pregnancy rate (69.4% vs. 40.0%, P = 0.035) and cumulative live birth rate (66.7% vs. 36.0%, P = 0.022) in dual trigger group.
Conclusion
For normal responders with low oocyte maturation rates, the dual trigger may be more effective than the conventional hCG trigger.
Trial Registration
Chictr.gov.cn, identifier: ChiCTR2100049292
MiRNA-625-3p as novel biomarker promotes cell proliferation and metastasis of lung adenocarcinoma by targeting KLF9
Authors: Qu Yunhui , Si Qiufang , Yang Ting , Liu Man , Du Renle , Ji Longtao , Zhang Xue , Su Jiao , Sun Hao , Wang Longzhi , Zhao Chunling , Liu Jingjing , Dai Liping , Ouyang Songyun 
Published: March 2023

Abstract
Background. MicroRNAs (miRNAs) are emerging as potential blood-based biomarkers and involved carcinogenesis for various cancers, including lung adenocarcinoma (LUAD).
Methods. In the present study, microarray was used to screen 2,549 miRNAs in serum samples (7 LUAD and 7 healthy samples). qRT-PCR was used to validate the expression of miRNA in serum samples from 30 LUAD and 30 normal individuals. Area under the receiver operating characteristic curve (AUC) was performed to evaluate diagnostic capability of miR-625-3p. Cell counting kit-8 assay (CCK-8) and Transwell assays were used to explore cell proliferation, migration and invasion. Bioinformatics prediction was applied in the search for the target genes of miR-625-3p. qRT-PCR, western blot and dual luciferase assay were used to validate the target gene of miR-625-3p. A xenograft tumor model was established to evaluate cell proliferation in vivo.
Results. MiR-625-3p was the most increased miRNA expressed in LUAD serum samples according to microarray analysis, which was verified in an independent serum sample, as well as in tissues based on TCGA database. And serum miR-625-3p provided a high diagnostic accuracy of LUAD (AUC=0.790, 95%CI:0.6640-0.9152). Functionally, miR-625-3p promoted LUAD cell proliferation, migration and invasion both in vivo and in vitro. Mechanistically, we identified KLF9 as an essential direct target of miR-625-3p, miR-625-3p promoted cell proliferation and metastasis of LUAD by targeting KLF9.
Conclusions. Our study identified that miR-625-3p plays an oncogenic role in LUAD, which promoted proliferation and migration through targeting KLF9. MiR-625-3p might be a potential novel diagnostic biomarker and target for LUAD therapy.
Delineation of Functional Changes and Associated Cortical Transcriptomic Profiles for Autism Spectrum Disorders
Authors: Wang Jiaojian , Li Yanling , Li Rui , Yang Jia , Ma Yingzi , Su Jing , Yu Xiaohui , Chen Kexuan , Lu Fengmei , Chen Yongchang 
Published: March 2023

Abstract
Background: Although abnormal functional activities or connectivities in individuals with autism spectrum disorders (ASD) have been reported, yet, there is still lack of consistent findings of altered functional activities or connectivities and its associated genetic basis for ASD.
Methods: Here, we aimed to identify consistent brain functional alterations and the biological genetic mechanisms in children and adults with ASD using a large sample of resting-state functional magnetic resonance imaging data from the Autism Brain Image Data Exchange dataset (ABIDE). Multi-site based meta-analyses were first employed and identified abnormal brain activity, local and global functional connectivities primarily localizing in frontal-temporal-occipital regions and default mode network (DMN) in individuals with ASD.
Results: The significant correlations between the aberrant brain regions and clinical phenotypes related to social functions in ASD were found. Importantly, brain-wide transcriptome-neuroimaging spatial association analyses and the follow-up enrichment, tissue-specific expression, and protein-protein interaction analyses were conducted and identified the underlying cellular and molecular mechanisms of ASD.
Conclusions: Collectively, our findings link molecular and functional changes in ASD and provide new evidence for neuropathology and a potential precious treatment target for ASD.
Uptake and Barriers to Cervical Cancer Screening among Human Immunodeficiency Virus-Positive Women in Sub Saharan Africa: A Systematic Review and Meta-Analysis
Authors: Mengesha Meresa Berwo , Chekole Tesfaye Temesgen , Hidru Hagos Degefa 
Published: March 2023

Abstract
Background
Cervical cancer is a leading cause of disability and mortality among women in Africa. Despite significant correlation between HIV/AIDS and cervical cancer, unacceptably low coverage of uptake of cervical cancer screening among Human Immunodeficiency Virus-positive women in Sub-Saharan Africa. Individual primary studies are limited in explaining the patterns of uptake of cervical cancer screening. Hence, this review considers the uptake of cervical cancer screening and its barriers among Human Immunodeficiency Virus-positive women in Sub-Saharan Africa.
Methods
We systematically searched articles published up to December 31st, 2019 from databases of PubMed, Cochrane Library, POP LINE, Google Scholar, African Journals Online and JURN. Quality of included articles was assessed by using the Newcastle-Ottawa Scale and the coverage of uptake of cervical cancer screening was pooled after checking for heterogeneity and publication bias. The random effect model was used and sub-group analysis estimate was done by countries.
Results
Twenty-one studies comprised of 20,672 Human Immunodeficiency Virus-Positive women were included. Applying random effect model, the overall cervical cancer screening uptake among this group of women in Sub Saharan Africa was estimated to be 30% (95% CI: 19, 41, I2 = 100%). The main barriers to uptake of cervical screening to include: poor knowledge about cervical cancer and screening, low risk perception of cervical cancer, fear of test result and fear of screening as painful, lack of access to screening services, high cost of screening service, and poor partner attitude and acceptance of the service. The perception of an additional burden of having a cervical cancer diagnosis was found to be a unique barrier among this population of women.
Conclusion
The review revealed that cervical cancer screening uptake is low due to poor knowledge about cervical cancer and screening, low risk perception of cervical cancer, Fear of test result and fear of screening, lack of access to screening services, high cost of screening services and poor partner attitude and acceptance of the service. Besides the above, perception of an additional burden of having a cervical cancer was found to be a unique barrier for these group of population.
The efficacy and safety of conscious sedation applied with midazolam in the transesophageal echocardiography procedure
Authors: Harbalıoğlu Hazar , Yıldırım Abdullah , Koca Fadime , Nacar Halil , Ucak Dilek , Koca Hasan 
Published: March 2023

Abstract

Objective The aim of this study was to compare the anaesthesia parameters, procedure duration, and the effect on hemodynamics and clinical parameters during the procedure in patients undergoing transesophageal echocardiography (TEE) with conscious sedation applied with midazolam or propofol. Methods This cross-sectional study included 401 patients (198 males, 203 females, mean age 52.9 ± 14.8 years) applied with TEE in our clinic. The demographic, clinical and laboratory parameters of the patients were recorded before the procedure. A record was made of pre-procedure ASA score and basal SaO2, the time to sedation to TEE, TEE duration, time to recovery, and during the procedure the minimum SaO2, the need for non-invasive mechanical ventilation (NIMV), the change in systolic and diastolic blood pressure (SBP and DBP), O2saturation change, and pulse change. The data were compared between the patients in two groups according to the conscious sedation agent used; midazolam and propofol. Results The demographic, clinical, and laboratory data of the midazolam and propofol groups were found to be similar (p > 0.05 for each). The procedure duration, and time to recovery were determined to be significantly shorter in the midazolam group than in the propofol group, and the time to onset of the sedation effect was significantly longer (p < 0.05 for each). Of the respiratory parameters, the minimum SaO2during the procedure, the absolute change in O2saturation, and the need for NIMV were determined to be significantly lower in the midazolam group (p < 0.05 for each). The absolute pulse and change in SBP and DBP values were found to be significantly higher in the propofol group (p < 0.05 for each). Conclusions The study results demonstrated that conscious sedation applied with midazolam during TEE can be applied with a shorter procedure duration and better hemodynamic and clinical results compared to sedation with propofol.
Enumeration module for human visual system: a recurrent on-center off-surround neural network approach
Authors: Verma Bhavesh , Sengupta Rakesh 
Published: March 2023

Abstract
Humans have an inherent ability to visually perceive small numerosities (the cardinality of a set). According to several studies, the LIP and other IPS regions of the brain include the neurological substrates for the processes related to numbers. Computational models play a crucial role in investigating the computations and dynamics behind the perception of numbers. Existing models of number perception simulate a few of the fundamental aspects of number perception, such as size and distance effects. However, most models usually work for a limited range of numbers and lack explanations for important behavioral characteristics like adaptation effects.In this study, we use a network of neurons with self-excitatory and mutual inhibitory properties to build a computational model of number perception. We assume that the network's mean activation at steady-state can encode numerosity when it increases monotonically with Setsize (the input to the network). By optimizing the total number of inhibition strengths required so that the combined monotonic regions cover the full stretch of numbers, we get three ranges of numbers (1:4, 5:17, and 21:50). This division of numbers into three parts closely matches the elbows in numerosity perception discovered in behavioral studies. Later in the study, we devised a method to decode the mean activation into a continuous scale of numbers ranging from 1 to 50. Furthermore, we suggest a mechanism for selecting inhibition strength based on current inputs, allowing the network to work for the entire range of numerosities. Our model provides novel perspectives on how our brain can generate various behavioral phenomena, such as the influences of continuous visual attributes and adaptation effects on perceived numerosity
Purification of Acetylcholinesterase from Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) and Management of Control of The Pest Through Inhibition of The Enzyme
Authors: Dinçer Barbaros , AKPINAR Havva 
Published: March 2023

Abstract
Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) has brought about agricultural harm throughout the Eastern Black Sea coastline since 2017. It continues to come to a threat because there are no adequate studies on managing this pest. One of the major rein strategies of insecticides is acetylcholinesterase (ACHE) inhibition. Therefore, this study aims to investigate an alternative way to struggle H. halys by inhibiting ACHE. The ACHE was purified from H. halys using edrophonium-Sepharose 6B affinity chromatography and characterized by examining some kinetic properties. The molecular weight of the purified enzyme was determined by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and natural polyacrylamide gel electrophoresis. In addition, the inhibitory effects of tacrine and edrophonium chloride, and water extracts of olive leaf, walnut leaf and alder leaf on this ACHE were investigated. The acetylcholinesterase was purified 403-fold with an 83.3% yield. H. halys ACHE was found to have six subunits and a molecular weight of approximately 350 kDa. The ACHE’s Km, Vmax, and kcat values were assigned to be 0.02 ± 0.006 mM, 3,333.3 ± 481 EU.mg protein− 1, and 1070.2 ± 184 min− 1, respectively. All inhibitors highly inhibited of activity of h.halys ACHE. Especially, the fact that the water extracts of these plants are effective in ACHE inhibition is significant due to being environmentally amicable pesticides that may be benefited in the struggle with the pest.