bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combination of 3PO analog PFK15 and siPFKL efficiently suppresses the migration, colony formation ability, and PFK‐1 activity of triple‐negative breast cancers by reducing the glycolysis
Authors: Kashyap Akanksha , Umar Sheikh Mohammad , Dev J. R. Arundhathi , Mathur Sandeep R. , Gogia Ajay , Batra Atul , Deo S. V. S. , Prasad Chandra Prakash 
Published: July 2023

AbstractAmong all the subtypes of breast cancer, triple‐negative breast cancer (TNBC) has been associated with the worst prognosis. Recently, for many solid tumors (including breast cancer) metabolic reprogramming has appeared as a cancer cell hallmark, and the elevated glycolytic pathway has been linked to their aggressive phenotype. In the present study, we evaluated the prognostic and therapeutic relevance of PFKFB3 (6‐phosphofructo‐2‐ kinase/fructose‐2,6‐bisphosphatase) in TNBCs. Prognostic significance of PFKFB3 expression was evaluated in overall breast cancers as well as in TNBCs. PFKFB3 inhibitor (3PO potent analogue i.e., PFK15) cytotoxicity in TNBC cell lines (MDA‐MB‐231 and MDA‐MB‐468) was analyzed using an MTT (3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay. Cancer cell physiological characteristics like clonogenicity and migration were also investigated after PFK15 treatment. As fructose‐2,6‐bisphosphate (F‐2,6‐BP), has been associated with increased PFK‐1 activity, the effect of PFKFB3 inhibition by PFK15 was investigated on two major isoforms of phosphofructokinase‐1 (PFK‐1) in breast cancer, that is, phosphofructokinase‐platelet type (PFKP) and phosphofructokinase‐liver type (PFKL) (relevant to breast cancer). For PFKL inhibition, the siRNA approach was used. PFKFB3 expression was significantly correlated with inferior overall survival in breast cancer patients including TNBCs. PFK15 treatment in TNBC cells (i.e., MDA‐MB‐231 and MDA‐MB‐468) resulted in a decreased PFKP expression, thereby leading to reduced colony formation ability, migration rate, and extracellular lactate levels. However, to our surprise PFK15 treatment in both TNBC cells also resulted in elevated PFKL levels. Our results demonstrated that the combinatorial inhibition of PFK15 with siPFKL was more effective in TNBC cells, as it led to a decrease in colony formation ability, migration rate, extracellular lactate levels, and PFK‐1 activity when compared with individual treatments. Using bona fide PFKFB3 inhibitor, that is, AZ67, we further show that AZ67 treatment to TNBC cells has no effect either on the expression of PFKP and PFKL, or on the lactate production. In summary, our present in vitro study demonstrated that 3PO derived PFK15 mechanism of action is totally different from AZ67 in TNBC cells. However, we advocate that the PFK15–mediated inhibition (along with PFKL) on the TNBCs migration, colony formation, and PFK‐1 activity can be further explored for the therapeutic advantage of TNBC patients.
Varieties of development: On the plurality of political economies and how to harness it
Authors: Garcés‐Velástegui Pablo 
Published: July 2023

AbstractDevelopment models are disputed in the global South and North, as evidenced by recent protests advancing different desirable social ends and means. The diversity of development ideas suggests it is a wicked problem, requiring clumsy solutions. Employing grid group cultural theory, this article elucidates that plurality and indicates ways to harness it. Four irreducible, ideal‐typical notions of development are found: market‐led individual prosperity, state‐led system‐wide welfare, alternatives of community‐led equal welfare with ecological conservation and a mirage. Clumsy development models secure legitimacy and effectiveness by engaging all worldviews, including their distinct pathways to clumsiness or messy institutions.
The relationship between Black racial identity, military identity, and postdeployment reintegration of Black male veterans
Authors: Winters Keiana , Hutchins Darvelle , Nelson Leslie R. 
Published: July 2023

AbstractGiven the increasing rate of military service members who experience mental health issues during reintegration into civilian culture, the need for counselors who specialize in postdeployment reintegration practices for Black men persists. Moreover, a nuanced approach to understanding how best to provide culturally competent support in therapeutic contexts for Black male veterans, a psychologically vulnerable population due to historical experiences of oppression and discrimination, is worthy of additional consideration. This study explored the impact of Black U.S. men's (N = 94) racial and military identities on postdeployment reintegration. Results indicated a significant predictive relationship between Black racial identity, military identity, and postdeployment reintegration. Practical implications and directions for future research are discussed.
Antimicrobial properties of green synthesized novel TiO2 nanoparticles using Iranian propolis extracts
Authors: Tahmasebi Elahe , Mohammadi Mehdi , Yazdanian Mohsen , Alam Mostafa , Abbasi Kamyar , Hosseini Hamideh Mahmoodzadeh , Tavakolizadeh Maryam , Khayatan Danial , Hassani Zahra , Tebyaniyan Hamid 
Published: July 2023

AbstractThe oral antimicrobial and cytotoxic properties of green synthesized novel titanium dioxide nanoparticles (TiO2 NPs) using Iranian propolis extracts were investigated on oral bacteria and fibroblast cells. In this study, propolis was sampled, and alcoholic extracts were prepared. The TiO2 NPs were biosynthesized using propolis extracts. The synthesized TiO2 NPs were characterized by scanning electron microscope (SEM), X‐ray diffraction analysis, energy‐dispersive X‐ray (EDX), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering, ultraviolet–visible (UV–Vis), transmission electron microscope, Brunauer–Emmett–Teller, and zeta potential. MTT (3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyl tetrazolium bromide), minimal inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, biofilm formation, and degradation tests were studied to clarify the oral antimicrobial properties of green synthesized TiO2 NPs. According to the FTIR analysis, the propolis extract contained flavonoids and phenolic compounds in addition to TiO2 NPs. Additionally, UV‐Vis revealed that intense bands had formed NPs. EDX spectra and SEM images revealed that the stabilizing agent was in perfect quasi‐spherical shapes around 21 nm. An EDX spectrum was used to verify the presence of titanium and oxygen. There were no significant cytotoxicity effects. The antibacterial results showed that Pro1TiO2 (Khalkhal sample) had better effects than Pro2TiO2 (Gilan sample) and TiO2 NPs. The present study presents a new process for synthesizing TiO2 NPs from propolis extracts with less toxic effects and user‐friendly, eco‐friendly, and economical materials. Pro1TiO2 NPs may be considered the best candidate for clinical application.
Foot kinematics as a function of ground orientation and weightbearing
Authors: Conconi Michele , Pompili Alessandro , Sancisi Nicola , Durante Stefano , Leardini Alberto , Belvedere Claudio 
Published: July 2023

AbstractThe foot is responsible for the bodyweight transfer to the ground, while adapting to different terrains and activities. Despite this fundamental role, the knowledge about the foot bone intrinsic kinematics is still limited. The aim of the study is to provide a quantitative and systematic description of the kinematics of all bones in the foot, considering the full range of dorsi/plantar flexion and pronation/supination of the foot, both in weightbearing and nonweightbearing conditions. Bone kinematics was accurately reconstructed for three specimens from a series of computed tomography scans taken in weightbearing configuration. The ground inclination was imposed through a set of wedges, varying the foot orientation both in the sagittal and coronal planes; the donor body‐weight was applied or removed by a cable‐rig. A total of 32 scans for each foot were acquired and segmented. Bone kinematics was expressed in terms of anatomical reference systems optimized for the foot kinematic description. Results agree with previous literature where available. However, our analysis reveals that bones such as calcaneus, navicular, intermediate cuneiform, fourth and fifth metatarsal move more during foot pronation than flexion. Weightbearing significantly increase the range of motion of almost all the bone. Cuneiform and metatarsal move more due to weightbearing than in response to ground inclination, showing their role in the load‐acceptance phase. The data here reported represent a step toward a deeper understanding of the foot behavior, that may help in the definition of better treatment and medical devices, as well as new biomechanical model of the foot.
A multimodal assessment of cementless tibial baseplate fixation using radiography, radiostereometric analysis, and magnetic resonance imaging
Authors: Broberg Jordan S. , Koff Matthew F. , Howard James L. , Lanting Brent A. , Potter Hollis G. , Teeter Matthew G. 
Published: July 2023

AbstractFixation in cementless total knee arthroplasty is provided by osseous integration. Radiography, radiostereometric analysis (RSA), and magnetic resonance imaging (MRI) were used simultaneously to investigate fixation. Relationships between RSA‐measured implant micromotions and MRI‐evaluated osseous integration at the component–bone interface were assessed in 10 patients up to 6 months postoperation. Supine MRI (using multispectral imaging sequences) and RSA exams were performed to evaluate osseous integration and measure longitudinal migration, respectively. Inducible displacement was measured from standing RSA exams. Radiolucent lines were detected on conventional radiographs. Of 10 patients, 6 had fibrous membranes detected on MRI. No fluid or osteolytic interfaces were found, and no components were scored loose. Of 10 patients, 6 had radiolucent lines detected. Average maximum total point motion (MTPM) for longitudinal migration at 6 months was 0.816 mm (range 0.344–1.462 mm). Average MTPM for inducible displacement at 6 months was 1.083 mm (range 0.553–1.780 mm). Fictive points located in fibrous‐classified baseplate quadrants had greater longitudinal migration than fictive points located in baseplate quadrants with normal interfaces at 2 weeks (p = 0.031), 6 weeks (p = 0.046), and 3 months (p = 0.047), and greater inducible displacements at 3 months (p = 0.011) and 6 months (p = 0.045). Greater early micromotion may be associated with the presence of fibrous membranes at the component–bone interface. Clinical significance: This multimodal imaging study contributes knowledge of the fixation of modern cementless TKA, supporting the notion that osseous integration is important for optimal implant fixation.
Surface‐enhanced Raman spectroscopy combined with multivariate analysis for the detection and differentiation of dopamine and DOPAC in cerebrospinal fluid
Authors: Pimiento Paula A. , Dunn Natalie E. , Sharma Bhavya 
Published: July 2023

AbstractDopamine (DA) is a vital neurotransmitter for brain functions, including pleasure, memory, motivation, and movement control. Dopaminergic neuron degradation is correlated to neurological disorders such as Parkinson's disease, and changes in DA concentration are related to mental health disorders. Currently, the gold standard for monitoring DA concentrations in the brain is electrochemistry. However, although electrochemistry has high sensitivity, it has low specificity for molecules with similar molecular structures, such as DA and its metabolites. Here, we demonstrate that surface‐enhanced Raman spectroscopy (SERS), a vibrational spectroscopy that provides molecule‐specific information with excellent sensitivity, requires small sample sizes/volumes, and is non‐destructive, is optimal for the detection and differentiation of DA and its metabolites. We show that SERS combined with multivariate analysis allows for the detection and differentiation of DA and its metabolites, including 3‐O‐methyldopamine (3‐OMD), homovanillic acid (HVA), 3,4‐dihydroxyphenylacetic acid (DOPAC), and 3‐methoxytyramine (3‐MT) at physiologically relevant concentrations in artificial cerebrospinal fluid (aCSF).
Simultaneous resection of colorectal cancer and synchronous colorectal liver metastases: A risk stratified analysis of the NSQIP database
Authors: Radomski Shannon N. , Chen Sophia Y. , Stem Miloslawa , Done Joy Z. , Efron Jonathan E. , Safar Bashar , Atallah Chady 
Published: July 2023

AbstractBackground and ObjectivesOver 25% of patients diagnosed with colorectal cancer (CRC) will develop colorectal liver metastases (CRLM). Controversy exists over the surgical management of these patients. This study aims to investigate the safety of a simultaneous surgical approach by stratifying patients based on procedure risk and operative approach.MethodsUsing ACS‐NSQIP (2016–2020), patients with CRC who underwent isolated colorectal, isolated hepatic, or simultaneous resections were identified. Colorectal and hepatic procedures were stratified by morbidity risk (high vs. low) and operative approach (open vs. minimally invasive). Thirty‐day overall morbidity was compared between risk matched isolated and simultaneous resection groups.ResultsA total of 65 417 patients were identified, with 1550 (2.4%) undergoing simultaneous resections. A total of 1207 (77.9%) underwent a low‐risk colorectal and low‐risk liver resection. On multivariate analysis, there was no significant difference in overall morbidity between patients who had a simultaneous open high‐risk colorectal/low‐risk hepatic procedure compared to patients who had an isolated open high‐risk colorectal procedure (odds ratio: 1.19; 95% confidence interval: 0.94–1.50; p = 0.148). All other combinations of simultaneous procedures had statistically significant higher rates of morbidity than the isolated group.ConclusionsSimultaneous resection of colorectal and synchronous CRLM is associated with an increased risk of morbidity in most circumstances in a risk stratified analysis, although rates of readmission and reoperation were not increased. Minimally invasive surgical approaches may significantly mitigate this morbidity.
Five‐year survival of resectable stage IIIA non‐small cell lung cancer in Brazil
Authors: de Barros Maria Laura Gritti Paes , Silva Vinícius G. , Moreira Frederico Rafael , Perez Stela V. , Younes Riad N. , Abrão Fernando Conrado 
Published: July 2023

AbstractBackgroundIn stage IIIA non‐small cell lung cancer (NSCLC), surgery plays a role in terms of multimodal treatment. Surgery rates have increased in recent years, mainly due to the combination of more accurate imaging tools, electromagnetic navigation bronchoscopy, robotic bronchoscopy, robotic surgery, and a wide range of challenging clinical scenarios to lead surgeons and oncologists to include surgery as an option in therapeutic management.ObjectivesTo assess the prognostic factors, the 5‐year overall survival (OS) and cancer‐specific survival (CSS) of patients with resectable stage III‐NSCLC.MethodsPatients' information was extracted from 76 Hospitals' Cancer Registry. OS and CSS were constructed using the Kaplan–Meier method, and the log‐rank test was used to assess differences between curves. In addition, Cox regression was conducted to evaluate the patients' characteristics leading to better OS and CSS.ResultsOverall, 433 stage III NSCLC surgical patients followed over 19 years were included. The median age was 61.29 ± 9.62 years, 58.4% male, 50.1% with adenocarcinoma, 29.3% with squamous cell carcinoma, 3.7% with large‐cell lung carcinoma, and 16,9% with other lung cancer types. The 5‐year OS was 30.6% (95% confidence interval [CI]: 27.4–36.1), and the CSS was 35.0% (95% CI: 29.4–41.0). In the Cox multivariate regression, squamous cell carcinoma was associated with reduced OS (hazard ratio [HR]: 1.40; 95% CI: 1.07–1.83; p=0.014) and CSS (HR: 1.56; 95% CI: 1.17–2.08; p = 0.002), in comparison with adenocarcinoma. The 2015–2019 quinquennial had a 50% reduction in HR (0.49; 95% CI: 0.29–0.81; p = 0.006), and the 2010–2014 group had a 40% reduction (0.59; 95% CI: 0.42–0.83; p = 0.006) in comparison with the 2000–2004 patients' group.ConclusionThe OS and CSS of patients with resectable stage III NSCLC have improved over the past 19 years in our region. Squamous cell carcinoma was associated with increased mortality risk from any cause or specific cancer.
An optimized extraction and gas chromatography analysis method for the quantification of diluent hydrocarbons in froth treatment tailings
Authors: Heshka Nicole E. , Rathie Kara , Degenhardt Dani 
Published: July 2023

Froth treatment tailings are one type of waste stream generated during the extraction of surface‐mined oil sands bitumen. To remove water and solids from bitumen froth recovered during the water‐based extraction process, hydrocarbon diluent is added, and settling and/or centrifugation are applied to the diluted bitumen froth, producing diluted bitumen and froth treatment tailings. While recovery processes are in place to remove and recycle the diluent from froth treatment tailings, some residual diluent can remain. Since tailings are stored in outdoor ponds, the residual diluent can have implications for methanogenic microbial processes and resulting greenhouse gas emissions. This work presents a methodology to accurately extract and quantify diluent hydrocarbons from froth treatment tailings using gas chromatography. A cold‐start temperature program is used to separate diluent hydrocarbons from any residual bitumen in the sample, and diluent is quantified using commercial standards as well as unprocessed diluent. A series of extraction parameters were tested and results from multiple conditions are shown with a rationale for the selected optimized parameters. Quantification of diluent in tailings samples is demonstrated from 60 to 5329 μg/g, and results from quality control standards show an average diluent recovery of 100 ± 10%.
The Diagnostic and Prognostic Value of EBUS‐TBNA for Intrathoracic Metastasis in Previously Treated Patients With Head and Neck Cancer
Authors: Li Sheng‐Ping , Zhang Zhen‐Ming , Bao Yu , Zhou Ling‐Xiao , Zhao Rui , Li Ji‐Man , Zhou Ye‐Han , Chen Xi , Hu Shang‐Zhi , Liu Wu‐Song 
Published: July 2023

ObjectiveThe present study assessed the diagnostic and prognostic significance of endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) for suspected intrathoracic metastasis after HNC treatment.MethodsA retrospective analysis was conducted on 75 patients with a prior history of head and neck cancer treatment who underwent EBUS‐TBNA for suspected intrathoracic metastases between March 2012 and December 2021.ResultsA total of 126 targeted lesions, including 107 mediastinal/hilar lymph nodes and 19 intrapulmonary/mediastinal masses, were sampled. The metastatic head and neck cancer (HNC) cases detected by EBUS‐TBNA consisted of nasopharyngeal carcinoma (n = 24), oropharyngeal carcinoma (n = 3), hypopharynx carcinoma (n = 6), laryngeal carcinoma (n = 6), and oral cavity carcinoma (n = 6). Cases with negative EBUS‐TBNA results consisted of tuberculosis (n = 9), sarcoidosis (n = 3), anthracosis (n = 9), and reactive lymphadenitis (n = 9). Six false‐negative cases were found among the 75 patients with suspected intrathoracic metastases. The diagnostic sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of the EBUS‐TBNA procedure for metastatic HNC were 88.2, 100.0, 100.0, 80, and 92.0%, respectively. The diagnosis of HNC intrathoracic metastasis by EBUS‐TBNA correlated with an adverse prognosis in terms of overall survival (OS) (P = .008). The log‐rank univariate analysis and Cox regression multivariate analysis results indicated that the detection of metastatic HNC through EBUS‐TBNA was a significant independent prognostic factor for patients with HNC who had received prior treatment.ConclusionsEndobronchial ultrasound‐guided transbronchial needle aspiration is a safe, effective, and minimally invasive procedure for assessing suspected intrathoracic metastasis in HNC patients after treatment. The intrathoracic metastasis detected by EBUS‐TBNA has crucial prognostic significance in previously treated HNC patients.
Risk of inpatient epistaxis admission related to oral anticoagulation medication use
Authors: Mitchell Margaret B. , Workman Alan D. , Lu Richard , Bhattacharyya Neil 
Published: July 2023

AbstractWe utilized a case control study to determine if novel oral anticoagulants were associated with a higher risk of inpatient epistaxis admission. Adult patients admitted with a principal diagnosis of epistaxis in 2019–2021 were identified as well as a control group of patients matched 1:1 for age, sex, race, and medical comorbidities. For both cohorts, the presence or absence of an oral anticoagulant, classified as vitamin K inhibitors, direct oral anticoagulants (DOAC) or platelet inhibitors, was identified. 158 adult unique inpatient admissions with a principal diagnosis of epistaxis were identified. Vitamin K inhibition was present in 5.7% of cases versus 0.6% of controls (p = 0.02; OR 9.48, range 1.19‐75.77), DOACs in 4.4% of cases versus 5.1% of controls (p = 1.0) and platelet inhibitors in 2.5% of cases versus 3.8% of controls (p = 0.75). We concluded vitamin K inhibitors, compared to DOACs and platelet inhibitors, may be associated with higher likelihood of epistaxis admission.
Deployment of cisplatin in Veterans with oropharyngeal cancer: toxicity and impact on oncologic outcomes
Authors: Soliman Ola , Wilde David C. , Kemnade Jan O. , Sabichi Anita L. , Chen George , Chen Albert , Little Samantha N. , Huang Andrew T. , Hernandez David J. , Sandulache Vlad C. 
Published: July 2023

AbstractObjectiveCisplatin forms the backbone of systemic chemotherapy treatment for oropharyngeal squamous cell carcinoma (OPSCC). The ideal cisplatin dosing regimen remains yet to be fully defined for achieving optimal efficacy and toxicity profiles in patients with comorbidity.MethodsWe retrospectively reviewed oncologic and toxicity data for patients with OPSCC treated at the Michael E. DeBakey Veterans Affairs Medical Center between 2000 and 2020 who initiated curative intent, definitive chemo‐radiation with one of three single agent regimens: high dose (HD) cisplatin, low dose (LD) cisplatin or cetuximab.ResultsPatients with HPV‐associated tumors and nonsmokers demonstrated improved overall and disease‐free survival along with locoregional and distant metastatic control regardless of chemotherapy regimen. Regardless of regimen selection, patients which received a cumulative cisplatin dose ≥200 mg/m2 had a lower rate of distant metastasis. The HD regimen resulted in a greater fraction (75% vs. 50%) of patients receiving a cumulative cisplatin dose ≥200 mg/m2 and a comparable measured toxicity burden compared to the LD regimen.ConclusionsBoth HD and LD cisplatin regimens can be safely delivered to a Veteran OPSCC patient population which should allow for straightforward application of conclusions drawn from completed and active clinical trials testing cisplatin regimens.Level of Evidence4.