bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENINGKATAN KOMPETENSI GURU DALAM MENGGUNAKAN APLIKASI PENILAIAN MELALUI KEGIATAN BIMBINGAN TEKNIS (BIMTEK) DI SMP NEGERI 1 TEWEH SELATAN TAHUN PELAJARAN 2021/2022
Authors: Hanil Hanil 
Published: January 2023

Realita yang terjadi di SMP Negeri 1 Teweh Selatan adalah Kompetensi Guru Dalam menggunakan aplikasi penilaian masih rendah, hal ini di tunjukkan data bahwa guru masih gaptek dengan penggunaan TIK atau belum bisa menggunakan aplikasi penilaian, Oleh karenanya peneliti disini yang sekaligus sebagai kepala sekolah hendak meningkatkan Kompetensi guru dalam menggunakan aplikasi penilaian melalui kegiatan bimbingan teknis (BIMTEK).Penelitian ni bertempat di SMP Negeri 1 Teweh Selatan. Subjek penelitian disini adalah guru yang ada di SMP Negeri 1 Teweh Selatan. Waktu penelitian dilakukan pada bulan September 2022 sampai dengan November 2022. Teknik pengumpulan data pada penelitian ini menggunakan teknik non tes. Teknik nontes merupakan teknik pengumpulan data yang tidak baku dan hasil rekayasa dari guru dan sekolah.Berdasarkan penilaian kompetensi guru dalam menggunakan aplikasi penilaian pada siklus I mendapatkan skor rata-rata 3,artinya kompetensi guru dalam menggunakan aplikasi penilaian dalam kategori baik. Terbukti terdapat peningkatan Kompetensi guru dalam menggunakan aplikasi penilaian yang dilihar dari penilaian rata-rata Kompetensi guru dalam menggunakan aplikasi penilaian semula pada kondisi pra siklus menunjukkan rata-rata penilaian yang cukup baik meningkat menjadi baik.Berdasarkan penilaian Kompetensi guru dalam menggunakan aplikasi penilaian pada siklus II dapat diketahui bahwa skor rata-rata menunjukkan nilai 5,37 atau masuk dalam kategori sangat baik. Dengan demikian dapat diketahui bahwa Kompetensi guru dalam menggunakan aplikasi penilaian yang ada di SMP Negeri 1 Teweh Selatan sudah sangat baik. Pelaksanaan kegiatan bimbingan teknis (BIMTEK) yang dilakukan secara terencana terbukti dapat meningkatkan Kompetensi guru dalam menggunakan aplikasi penilaian di SMP Negeri 1 Teweh Selatan.
Climate Change as a Challenge to the Armed Forces
Authors: Földi László , Padányi József 
Published: January 2023

Abstract
Threats to national security are becoming more and more diverse, and factors are emerging which we did not expect. In recent decades, we have increasingly perceived extreme environmental events resulting from climate change as threats. This includes natural disasters related to weather, climate and/or water (windstorms, extreme precipitation, heat waves and extreme cold, wildfires, droughts,.). Military forces are carrying out their operations while being more and more affected by difficult environmental circumstances, and in addition their use in disaster management has become indispensable in our times. In this paper, the authors examine climate-related disasters’ impact on national security and on the use of military forces.
The impact of zygote vitrification timing on pregnancy rate in frozen-thawed IVF/ICSI cycles
Authors: Makieva Sofia , Stähli Celine , Xie Min , Gil Ana Velasco , Sachs Maike Katja , Leeners Brigitte 
Published: January 2023

Introduction: Cryopreservation of bipronuclear (2PN) stage zygotes is an integral part of IVF laboratory practice in countries with strict embryo culture legislation. Vitrification of zygotes is compatible with several strategies in infertility treatments holding a freeze-all indication and allows for effective workload management in settings with limited resources. Although it yields high survival rates and clinical outcomes, the ideal timing to commence vitrification of zygotes is elusive while it is empirically practiced in the window between 17 and 21 h post-insemination (hpi). We aimed to deduce the association between pregnancy rate and the time interval from insemination (IVF and ICSI) to vitrification to contribute to the standardization ofzygote cryopreservation.Methods: A retrospective analysis of data on vitrification timings and pregnancy outcomes collected between 2011 and 2019 was performed. All included women received an embryo transfer after warming of vitrified zygotes at the 2PN stage.Results: A total of 468 embryo transfers were included of which 182 (38.9%) resulted in pregnancy and 286 (61.1%) not. Vitrification was on average performed 18.74 ±0.63 hpi in the pregnant group and 18.62 ± 0.64 hpi in the non-pregnant group (OR 1.36, 95% CI 1.01; 1.83, p = 0.045). A multivariate analysis controlling for uterine pathologies, maternal age, AMH, the number of MII oocytes, previous history of pregnancy success, endometriosis, AFC, nicotine intake and male factor infertility showed no predictive value of vitrification timing on pregnancy rate. Three time intervals between insemination and vitrification were defined: 17:00 to 18:00 hpi (Group A), 18:01 to 19:00 hpi (Group B) and 19:01 to 21:00 hpi (Group C). Pregnancy occurred in 40/130 women (30.80%) in Group A, in 115/281 women (40.90%) in Group B and in 27/57 women (47.40%) in Group C. Univariate but not multivariate analysis showed a significantly higher pregnancy rate after the latest time interval between insemination and 2PN vitrification when compared to the earliest (Group C vs. A, OR 2.03, 95% CI 1.07; 3.84, p = 0.031).Discussion: These findings encourage further investigation on the impact of vitrification timing on clinical outcomes and hold the potential to standardize cryopreservation of zygotes from IVF/ICSI cycles to eventually improve the quality of long-term ART outcomes.
Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest
Authors: de Macedo Gabriel Carvalho , Barreto Wanessa Teixeira Gomes , de Oliveira Carina Elisei , Santos Filipe Martins , Porfírio Grasiela Edith de Oliveira , Xavier Samanta Cristina das Chagas , Alves Fernanda Moreira , da Silva Alanderson Rodrigues , de Andrade Gisele Braziliano , Rucco Andreza Castro , de Assis William Oliveira , Jansen Ana Maria , Roque André Luiz Rodrigues , Herrera Heitor Miraglia 
Published: January 2023

IntroductionThe aim of the present study was to investigate the occurrence of Leishmania infantum in South American coatis inhabiting two forest fragments in Campo Grande, Mato Grosso do Sul, Midwest region of Brazil, an endemic area of human and canine visceral leishmaniasis (VL).Material and methodsA total of 110 South American coatis were sampled in the conservation unit “Parque Estadual do Prosa” (PEP) and in the residential area “Vila da Base Aérea” (VBA) from March 2018 to April 2019. As a longitudinal study that include up to six recaptures of the same individual, a total of 190 capture events were obtained. Blood, bone marrow and skin samples were obtained for parasitological (axenic culture), serological (Enzyme Linked Immunosorbent Assay – ELISA and Dual-path Platform immunoassay – DPP® CVL) and molecular diagnostic assays (targeting kDNA for Leishmania spp. and L. infantum; and HSP70 followed by sequence analysis).ResultsSeropositivity for L. infantum was found in 33 individuals, six in PEP and 27 in VBA. Furthermore, L. infantum was detected by molecular analysis in 16 individuals, seven from PEP and nine from VBA. We also isolated L. infantum from bone marrow of one individual and detected a single positive skin sample in molecular assay from other individual, both from VBA.DiscussionAn overall infection rate of 36.4% (40/110) was observed, significantly higher in the VBA (49.1%) than in the PEP (21.6%), probably because VBA presents: (i) a large number of resident dogs and chickens that would be attracting sandflies; (ii) a denser population of this wild mammal species; and (iii) physical barriers and a lack of functional connectivity in the surroundings, preventing these animals to disperse out. We conclude that South American coati populations living in urban forest fragments of Campo Grande are affected by the epidemiological scenario of VL, known to involve dogs, vectors and humans. We highlight the importance of investigate the parasitism by L. infantum in this and other potential L. infantum reservoirs that inhabit urbanized regions endemic to VL.
Gut microbiota-dependent trimethylamine n-oxide pathway contributes to the bidirectional relationship between intestinal inflammation and periodontitis
Authors: Wang Qiqi , Sun Yue , Zhou Tianyu , Jiang Cong , A Lan , Xu Wenzhou 
Published: January 2023

BackgroundIntestinal inflammation and periodontitis influence the development of each other through the bidirectional relationship. As the intestinal microbiome metabolite, trimethylamine-N-oxide (TMAO) could contribute to chronic inflammation in the gut by influencing the gut microbial composition and intestinal immunity. Increased circulating TMAO levels often accompany clinical findings in patients with experimental periodontitis. However, the role of TMAO in the bidirectional relationship between intestinal inflammation and periodontitis remains unclear. Thus, we explored whether TMAO influences the periodontitis process by affecting intestinal immunity and microbial composition in this article.MethodsPeriodontitis was induced by unilateral ligation of the first molar in mice, and 3,3-dimethyl-1-butanol (DMB) was used as an inhibitor to reduce TMAO circulating. Twenty-five BALB/c mice were randomly assigned to five study sets (n = 5/group): no periodontitis with DMB (Control group), periodontitis (P) group, periodontitis with TMAO (P+TMAO) group, periodontitis with TMAO and DMB (P+TMAO+DMB) group, and periodontitis with DMB (P+DMB) group. The effect of TMAO was determined by assessing changes in intestinal histology, intestinal flora composition, periodontal tissue, and periodontal pro-inflammatory factors at ten days.ResultsThe outcomes indicated a marked improvement in the intestinal inflammation severity, and intestinal flora diversity was reduced. Firmicutes number and the ratio of Firmicutes/Bacteroidetes were improved in the P+TMAO group. In addition, the alveolar bone resorption and the degree of periodontal tissue inflammation were more severe in the P+TMAO group than in other groups. Immunohistochemistry showed higher levels of TGF-β and IL-1β expression in the periodontal tissues of P+TMAO.ConclusionsOur data suggest that TMAO could influence periodontal immunity and promote periodontal inflammation by affecting the intestinal microenvironment, revealing TMAO may affect the development of periodontitis through the bidirectional relationship of the oral-gut axis.
Application of geophysical prospecting methods ERT and MASW in the landslide of Daofu County, China
Authors: Zhang Gang , Tu Fangzhou , Tang Yushu , Chen Xingchang , Xie Kuilin , Dai Sen 
Published: January 2023

Natural disasters such as debris flow caused by earthquakes seriously threaten the local infrastructure and economy, as well as the lives of people in the area. As the material source of debris flow, it has significance to accurately and effectively study the underground structure of the landslide to prevent debris flow disasters. A landslide has a complex structural system, and its underground characteristics play an important role in its stability. The early identification of fracture surfaces and unstable bodies, and assessment of potential hazards are essential for prevention and protection. The research object of this paper is a landslide that occurred in Yige Village, Xianshui Town, Daofu County, which is on the Xianshui River Earthquake Zone, an area subject to frequent earthquakes. In western Sichuan, the frequent occurrence of landslides has caused considerable economic losses. Developing methods for efficient and accurate risk assessment is a top priority. The Daofu landslide is a typical example of a landslide directly threatening the road below and forming a debris flow channel. The lithology is composed of Jurassic sedimentary rocks, such as marl and clay, covered by limestone. In this study, we combined traditional methods (drilling and field investigation) with two geophysical techniques, multichannel analysis of surface waves (MASW) and electrical resistivity tomography (ERT) to effectively determine the electrical characteristics, velocity characteristics and spatial structure of the landslide. It is found that the buried depth of the sliding surface of the landslide is about 16–20 m. The sliding body above the sliding surface forms a low velocity and low resistivity Quaternary cover. The rock mass below the sliding surface is Triassic Zhuwo Formation sandstone and slate with high velocity and high resistivity. According to comprehensive analysis, the landslide lacks sufficient stability under rainstorm. Our study shows that the use of MASW and ERT can quickly and effectively characterize the subsurface of landslides to assess landslide risk and prevent debris flow hazards.
Improving human mesenchymal stem cell-derived hepatic cell energy metabolism by manipulating glucose homeostasis and glucocorticoid signaling
Authors: Rodrigues Joana Saraiva , Faria-Pereira Andreia , Camões Sérgio Póvoas , Serras Ana Sofia , Morais Vanessa Alexandra , Ruas Jorge Lira , Miranda Joana Paiva 
Published: January 2023

IntroductionThe development of reliable hepatic in vitro models may provide insights into disease mechanisms, linking hepatocyte dysmetabolism and related pathologies. However, several of the existing models depend on using high concentrations of hepatocyte differentiation-promoting compounds, namely glucose, insulin, and dexamethasone, which is among the reasons that have hampered their use for modeling metabolism-related diseases. This work focused on modulating glucose homeostasis and glucocorticoid concentration to improve the suitability of a mesenchymal stem-cell (MSC)-derived hepatocyte-like cell (HLC) human model for studying hepatic insulin action and disease modeling.MethodsWe have investigated the role of insulin, glucose and dexamethasone on mitochondrial function, insulin signaling and carbohydrate metabolism, namely AKT phosphorylation, glycogen storage ability, glycolysis and gluconeogenesis, as well as fatty acid oxidation and bile acid metabolism gene expression in HLCs. In addition, we evaluated cell morphological features, albumin and urea production, the presence of hepatic-specific markers, biotransformation ability and mitochondrial function.ResultsUsing glucose, insulin and dexamethasone levels close to physiological concentrations improved insulin responsiveness in HLCs, as demonstrated by AKT phosphorylation, upregulation of glycolysis and downregulation of Irs2 and gluconeogenesis and fatty acid oxidation pathways. Ammonia detoxification, EROD and UGT activities and sensitivity to paracetamol cytotoxicity were also enhanced under more physiologically relevant conditions.ConclusionHLCs kept under reduced concentrations of glucose, insulin and dexamethasone presented an improved hepatic phenotype and insulin sensitivity demonstrating superior potential as an in vitro platform for modeling energy metabolism-related disorders, namely for the investigation of the insulin signaling pathway.
Validation of dynamic risk stratification and impact of BRAF in risk assessment of thyroid cancer, a nation-wide multicenter study
Authors: Pérez-Fernández Laura , Sastre Julia , Zafón Carles , Oleaga Amelia , Castelblanco Esmeralda , Capel Ismael , Galofré Juan C. , Guadalix-Iglesias Sonsoles , De la Vieja Antonio , Riesco-Eizaguirre Garcilaso 
Published: January 2023

IntroductionThe dynamic risk stratification (DRS) is a relatively new system in thyroid cancer that considers the response to primary treatment to improve the initial risk of recurrence. We wanted to validate DRS system in a nationwide multicenter study and explore if the incorporation of BRAFV600E into DRS helps to better categorize and predict outcomes.Materials and methodsRetrospective study of 685 patients from seven centers between 1991 and 2016, with a mean age of 48 years and a median follow-up time of 45 months (range 23-77). The overall BRAFV600E prevalence was 53.4%. We classified patients into four categories based on DRS (‘excellent’, ‘indeterminate’, ‘biochemical incomplete’, and ‘structural incomplete’ response). Cox regression was used to calculate adjusted hazard ratios (AHR) and proportions of variance explained (PVEs).ResultsWe found 21.6% recurrences and 2.3% cancer-related deaths. The proportion of patients that developed recurrence in excellent, indeterminate, biochemical incomplete and structural incomplete response to therapy was 1.8%, 54%, 91.7% and 96.2% respectively. Considering the outcome at the end of the follow up, patients showed no evidence of disease (NED) in 98.2, 52, 33.3 and 25.6% respectively. Patients in the structural incomplete category were the only who died (17.7%). Because they have similar outcomes in terms of NED and survival, we integrated the indeterminate and biochemical incomplete response into one unique category creating the 3-tiered DRS system. The PVEs of the AJCC/TNM staging, ATA risk classification, 4-tiered DRS, and 3-tiered DRS to predict recurrence at five years were 21%, 25%, 57% and 59% respectively. BRAFV600E was significantly associated with biochemical incomplete response (71.1 vs 28.9%) (HR 2.43; 95% CI, 1.21 to 5.23; p=0.016), but not with structural incomplete response or distant metastases. BRAF status slightly changes the AHR values of the DRS categories but is not useful for different risk grouping.ConclusionsThis is the first multicenter study to validate the 4-tiered DRS system. Our results also show that the 3-tiered DRS system, by integrating indeterminate and biochemical incomplete response into one unique category, may simplify response to therapy keeping the system accurate. BRAF status does not provide any additional benefit to DRS.
Exploring perceptions, knowledge, and attitudes regarding pharmacogenetic testing in the medically underserved
Authors: Gawronski Brian E. , Cicali Emily J. , McDonough Caitrin W. , Cottler Linda B. , Duarte Julio D. 
Published: January 2023

Introduction: Pharmacogenetic testing may hold promise in addressing health disparities, as medically underserved patients appear to be prescribed medications with pharmacogenetic guidelines at higher rates. While routine clinical implementation of testing in medically underserved populations has not yet been achieved, using patient perspectives to inform implementation should increase the likelihood of success. The aim of this study was to assess the perceptions, knowledge, and attitudes regarding pharmacogenetic testing in medically underserved patients.Methods: We developed a survey instrument to assess respondent views on pharmacogenetic testing. The survey instrument was developed through a process of literature review, expert input, iterative pilot testing, and final refinement. The survey instrument was fielded to US adults with an estimated household income of $42,000 per year or less.Results: During the survey instrument development, 59 pilot testers provided 133 comments which lead to 38 revisions to the survey instrument. The nationwide survey resulted in 1,060 respondents, of which half (49.8%) reported having no health insurance or being on Medicaid. Most patients (78.9%) had not previously heard of pharmacogenetic testing. After being provided an explanation of pharmacogenetic testing, 60.5% were very or moderately interested in receiving testing if there were no cost and 75.8% of respondents agreed or strongly agreed that pharmacogenetic testing should be available to help with medication selection regardless of cost. Respondents shared that their greatest concern with pharmacogenetic testing was that the test would cost them money, which was expressed by over half (52.7%). This was followed by concerns that the results could reveal a risk for a disease, could affect health insurance, and would not improve care.Discussion: Our results indicate a strong interest in pharmacogenetic testing and identify key perceptions, attitudes, concerns, and potential barriers that can be addressed as pharmacogenetic testing is clinically implemented in medically underserved patient populations.
Chronic granulomatous disease associated with Duchenne muscular dystrophy caused by Xp21.1 contiguous gene deletion syndrome: Case report and literature review
Authors: Bi Shaohua , Dai Liying , Jiang Liangliang , Wang Lili , Teng Mia , Liu Guanghui , Teng Ru-Jeng 
Published: January 2023

Chronic granulomatous disease (CGD) and Duchenne muscular dystrophy (DMD) are X-linked recessive disorders whose genes are 4.47 Mb apart within Xp21.1. A combination of both diseases is rare with only five cases reported in the literature where it is known as Xp21.1 “contiguous gene deletion syndrome”. We describe a male neonate who presented with sepsis at 19 days of age. The diagnosis of CGD with DMD was established through copy number variation sequencing (CNV-seq) with an extensive 7.5 Mb deletion of Xp21.2-Xp11.4 of the proband. One of his elder sisters and his mother are carriers. The deletion includes six known genes: glycerol kinase (GK), dystrophin (DMD), cilia- and flagella-associated protein 47 (CFAP47), gp91 (CYBB), Kell antigen (XK), and retinitis pigmentosa GTPase regulator (RPGR). Laboratory assays revealed an increased creatine kinase (CK) level, decreased gp91 expression, and a positive nitroblue tetrazolium test. Due to the extensive gene deletion and the poor prognosis, the family determined to pursue conservative management without further laboratory workup. The patient passed away from a fulminant infection at the age of three-month at a local medical facility. To the best of our knowledge, this case of Xp21.1 contiguous gene deletion syndrome represents the most extensive deletion of genes in this region ever reported. A literature review of similar cases is presented.
Cuproptosis-related LncRNAs signature as biomarker of prognosis and immune infiltration in pancreatic cancer
Authors: Chen Hui , Yu Yang , Zhou Lei , Chen Junliang , Li Zeyu , Tan Xiaodong 
Published: January 2023

Background: Pancreatic cancer (PC) is a malignant gastrointestinal tumor with a terrible prognosis. Cuproptosis is a recently discovered form of cell death. This study is intended to explore the relationship between cuproptosis-related lncRNAs (CRLncs) signature with the prognosis and the tumor microenvironment (TME) of PC.Methods: Transcript sequencing data of PC samples with clinical information were obtained from the Cancer Genome Atlas (TCGA). Univariate Cox regression analysis and LASSO regression analysis were employed to construct the prognostic signature based on CRLncs associated with PC survival. A nomogram was created according to this signature, and the signaling pathway enrichment was analyzed. Subsequently, we explored the link between this prognostic signature with the mutational landscape and TME. Eventually, drug sensitivity was predicted based on this signature.Results: Forty-six of 159 CRLncs were most significantly relevant to the prognosis of PC, and a 6-lncRNA prognostic signature was established. The expression level of signature lncRNAs were detected in PC cell lines. The AUC value of the ROC curve for this risk score predicting 5-year survival in PC was .944, which was an independent prognostic factor for PC. The risk score was tightly related to the mutational pattern of PC, especially the driver genes of PC. Single-sample gene set enrichment analysis (ssGSEA) demonstrated a significant correlation between signature with the TME of PC. Ultimately, compounds were measured for therapy in high-risk and low-risk PC patients, respectively.Conclusion: A prognostic signature of CRLncs for PC was established in the current study, which may serve as a promising marker for the outcomes of PC patients and has important forecasting roles for gene mutations, immune cell infiltration, and drug sensitivity in PC.