bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the machining characteristics of Al6082 Hybrid metal matrix composites milled under cryogenic cooling conditions
Authors: Sivalingam Vinothkumar , Zhou Qian , Manickajothi Ganesh , Ross Nimel Sworna , Sun Jie , Gupta Munish Kumar , Korkmaz Mehmet Erdi , Nagamalai Thangapandian 
Published: July 2023

Abstract
Cutting fluid used in machining is under investigation nowadays to check the achievement of sustainability and the cleaner process by the machining process. Many attempts were made to find new cooling strategies as an alternative to the existing cooling methods. Cryogenic cooling was found to be an efficient cooling method to move towards sustainability for machining any kind of materials and to ensure green machining. Machining of Al6082 HMMC has been carried out under distinct cutting environments. The fallout of these cutting environments was studied based on the parameters like surface roughness, cutting temperature, chip morphology, and tool wear. The application of Cryogenic co2 substantially reduced the tool wear by 37% & 48% when compared to MQL and wet conditions. The sem image of the worn tool disclosed that the adoption of the cryogenic cooling technique lead to less crater wear and also prevented the peeling of the tool layer to some extent. The impressive cooling effect of cryogenic cooling also produced a good surface finish on the machined surface in comparison to the other cooling environments. Thus cryogenic cooling proved to be trustworthy in all the output parameters.
Investigation of the effect of anticoagulants on platelet recovery, enrichment factor, PDGF, TGF-β1 and optimal dose in the preparation of platelet-rich plasma
Authors: Chen Chao , Lei Jun , Yi Xinming , Hua ye , Yang Jun 
Published: July 2023

Abstract
Objective
To explore the effect of anticoagulant on platelet recovery and enrichment coefficient of platelet-rich plasma and the optimal dose.
Methods
Nine New Zealand rabbits were divided into 9 groups with 1 rabbit in each group. Platelet recovery rate, enrichment coefficient, platelet-derived factor (PDGF), transforming growth factor (TGF-β1) contents were observed.
Results
The enrichment coefficient and platelet recovery of conventional blood + 120% sodium citrate group were the highest, the high dose blood + 120% sodium citrate group was the lowest, the conventional blood + 120% sodium citrate group was the lowest, and the low dose blood + 80% sodium citrate group was the most. Before plasma activation, the expressions of PDGF and TGF-β1 were significantly increased in the other groups compared with the control group, and were higher in the high-concentration blood group. Compared with before activation, TGF-β1 was significantly decreased in the low concentration blood group after activation; TGF-β1 was significantly decreased in the high concentration blood + 80% sodium citrate group; PDGF showed a downward trend in the high concentration blood group; the expression of PDGF was significantly decreased in each group after activation; TGF-β1 was significantly increased in the conventional blood + 120% sodium citrate group. TGF-β1 was significantly decreased in patients treated with low concentration of 80%, 100%, 120% sodium citrate and high concentration of blood + 80% sodium citrate.
Conclusion
Anticoagulant 120% sodium citrate in conventional blood could separate more platelets, and the enrichment coefficient and platelet recovery rate were the highest, and the biological function was good.
Comparative Analysis and Predictability of Symmetric Division Degree Index on PCB Congeners
Authors: Rajpoot Abhay , Selvaganesh Lavanya 
Published: July 2023

Abstract
Vukičević and Gašperov, in 2010, introduced a potentially useful topological index known as the Symmetric division deg (SDD) index. They showed that it best correlates when predicting the total surface area of polychlorobiphenyls (PCB). In this article, we analyze the relationship between the SDD index with other physico-chemical properties of PCBs, such as log water solubility, octanol-water partition ratio, with the aid of computers.
We also compare it with other well-known vertex degree-based (VDB) indices. We show that the SDD-index has a better correlation ability with a value of 0.9749 for log-water solubility and 0.9475 for octanol-water partition coefficient. We also identify the expected values for these two properties of PCBs whose experimental values are not available.
Mathematics Subject Classification (2010) 92E10 · 05C12 · 05C05
Does Vaginal Cleansing with Iodine Decreases Infection Rate in Office Hysteroscopy?
Authors: Cohen Nadav , Kedem Hagit Itzhak , Abu-Foul Luna , Paz Moran , Kaufman Yuval , Lavie Ofer , Zilberlicht Ariel 
Published: July 2023

Abstract
Purpose: To determine whether preoperative vaginal cleansing with iodine-based solution prior to hysteroscopy, reduces postoperative infection rate compared to no vaginal cleansing.
Methods: A retrospective study at a single tertiary, university affiliated hospital of all individuals who have had an office hysteroscopy. Study group consisted of patients who have had preoperative vaginal cleansing with iodine-based solution and were compared to those who have not. Any procedural related complaints at the 30 days following the hysteroscopy were recorded.
Results: A total of 505 were available for analysis, 183 in the study group and 322 in the control group. The most common indications for operation were infertility evaluation (33.9% vs. 28.6%) and retained products of conception (33.3% vs. 29.2%), and most common procedures were diagnostic only (49.7% vs. 42.5%) followed by adhesiolysis (12% vs. 14.3%)
No statistical differences were noted with regards to postoperative infections, as only one case of pelvic inflammatory disease was noted, in the iodine cleansing group (0.19% for postoperative infection).
Conclusion: Our comparative study may suggest that the use of preoperative iodine solution prior to office hysteroscopy does not decrease the postoperative infection rate up to 30 days, thus can be avoided.
Intraventricular central neurocytoma molecularly defined as extraventricular neurocytoma: A case representing the discrepancy between clinicopathological and molecular classifications.
Authors: Sato Daisuke , Takami Hirokazu , Takayanagi Shunsaku , Ikemura Masako , Matsuura Reiko , Tanaka Shota , Saito Nobuhito 
Published: July 2023

Abstract
Central neurocytoma (CN) is classically defined by its intraventricular location, neuronal/neurocytic differentiation, and histological resemblance to oligodendroglioma. Extraventricular neurocytoma (EVN) shares similar histological features with CN, while it distributes any site without contact with the ventricular system. CN and EVN have distinct methylation landscapes, and EVN has a signature fusion gene, FGFR1-TACC1. These characteristics distinguish between CN and EVN.
A 30-year-old female underwent craniotomy and resection of a left intraventricular tumor at our institution. The histopathology demonstrated the classical findings of CN. Adjuvant irradiation with 60Gy followed. No recurrence has been recorded for 25 years postoperatively. RNA sequencing revealed FGFR1-TACC1 fusion and methylation profile was discrepant with CN but compatible with EVN.
We experienced a case of anatomically and histologically proven CN in the lateral ventricle. However, the FGFR1-TACC1 fusion gene and methylation profiling suggested the molecular diagnosis of EVN. The representative case was an “intraventricular” neurocytoma displaying molecular features of an “extraventricular” neurocytoma. Clinicopathological and molecular definitions have collided in our case and raised questions about the current definition of CN and EVN.
Microbial fermented feed and ginseng polysaccharide synergistically improves growth performance and immune parameters of Xuefeng black-bone chicken
Authors: Liu Jie , Wang Huan , Luo Junyi , Chen Ting , Xi Qianyun , Sun Jiajie , Wei Limin , Zhang Yongliang 
Published: July 2023

Abstract
Oral supplementation of Gps dosages (100 mg/kg, 200 mg/kg, and 400 mg/kg) eliminated weight loss in chickens following vaccination (H5N1). Microbial fermented feed (MF) is considered as a valuable strategy to bring advantages to livestock and is widely practiced. This study investigated the effects of Gps, MF, and combined use of Gps and MF on growth performance and immune indices in Xuefeng black-bone chickens. A total of 400 Xuefeng black-bone chickens at the age of 1 day were randomly as-signed to four groups, each group contained 5 pens per treatment and 20 animals per pen. Compared with the control group, the body weight and average daily gain in the Gps group, MF group, and MF+Gps group increased significantly, while the feed conversion ratio decreased significantly (P < 0.01). It is noteworthy that the combined use of MF and Gps showed a synergistic effect. Moreover, the diarrhea rate of the Gps group and the MF+Gps group was lower than that of the Con group, while that of the MF+Gps group decreased the mortality rate (P < 0.05). In this work, we also focused on the effects of MF, Gps and MF+Gps on immune function. Compared with the Con group, the serum TNF-α and IL-6 levels in the MF group, Gps group and MF+Gps group decreased significantly (P < 0.01), the serum IgG levels increased significantly (P < 0.01), while the combination of MF and Gps had a synergistic effect. In summary, Gps or MF can promote growth performance and immune indexes, and the combined use of Gps and MF not only further improved growth performance and immune parameters, but also reduced the diarrhea rate and mortality. Therefore, our research developed a new type of compound feed that could well improve the growth performance and immune parameters of chickens.
Identification of antifungal compound isolated from Cercospora sp. ME202 and evaluation of the inhibitory activity against Colletotrichum orbiculare
Authors: Ino Masatoshi , Kihara Junichi , Ishihara Atsushi , Seki Kota , Tanaka Tomoya , Yoshikiyo Keisuke , Ueno Makoto 
Published: July 2023

Abstract
Cucumber anthracnose, an important disease caused by Colletotrichum orbiculare, is controlled using fungicides. However, this can lead to the development of fungicide resistance. We have previously shown that an ethyl acetate extract of the culture filtrate of Cercospora sp. ME202 (ME202-ECF) exhibits fungicidal activity and inhibits the formation of anthracnose lesions caused by C. orbiculare. However, studies on the control of plant diseases using the antimicrobial compounds produced by Cercospora have not been conducted. Here, we aimed to isolate and identify antifungal compounds produced by Cercospora sp. ME202 and evaluate their antifungal activity against C. orbiculare. We identified traversianal, a tricyclic diterpenoid aldehyde with a molecular weight of 316.4 from ME202-ECF. The traversianal structure was determined by liquid chromatography-mass spectrometry using electrospray ionization and nuclear magnetic resonance spectroscopy after purification by column chromatography and high-performance liquid chromatography. The inhibitory activity of isolated traversianal was detected as a mycelial growth inhibition zone at Rf 0.92 by thin layer chromatography bio-assay. Traversianal also inhibited the conidial germination of C. orbiculare at 2–10 ppm and exhibited fungicidal activity at 5–10 ppm. Furthermore, pretreatment of cucumber leaves with 10, 20, and 30 ppm traversianal significantly suppressed lesion formation in C. orbiculare without phytotoxicity. These results indicate that the traversianal produced from Cercospora sp. ME202 is a potential candidate as a new fungicide against C. orbiculare.
Bony Bankart lesions are common. A study of 790 cases from the Swedish Fracture Register
Authors: Gordins Vladislavs , Sansone Mikael , Thorolfsson Baldur , Möller Michael , Carling Malin , Olsson Nicklas 
Published: July 2023

Abstract
Background: A bony Bankart lesion is a common type of fracture associated with anterior shoulder dislocation. This type of fracture does directly affect the stability of the shoulder by reducing the glenoid joint-contact area.
The aim of this study was to report on the epidemiological data relating to bony Bankart lesions in Sweden using the Swedish Fracture Register. The purpose is to evaluate age and sex distribution in the population with bony Bankart lesions, its impact on treatment strategy and further to analyse patient-reported outcomes.
Methods: An epidemiological descriptive study. The inclusion criteria were all patients with a unilateral bony Bankart lesion registered between April 2012 and April 2019. The patients’ specific data (age, sex, type and time of injury, treatment option and patient-reported outcomes) were extracted from the Swedish Fracture Register database.
Results: A total of 790 unilateral bony Bankart fractures were identified. The majority of the patients were male (58.7%). The mean age ± SD for all patients at the time of injury was 56.3 ± 16.1 years. Females had a higher mean age, 64.3 ± 12.8, compared with males, 50.7 ± 15.7.
Most of the bony Bankart lesions, 662 (91.8%), were registered as a low-energy trauma. More than two-thirds of all treatment registered cases, 509/734 patients (69.3%), were treated non-surgically, 225 (30.7%) were treated surgically, while, in 17 patients (7.5% of all surgically treated patients), the treatment was changed from non-surgical to surgical due to recurrent instability. Surgical treatment was chosen for 149 (35%) of the males and for 76 (25%) of the females. Patient quality of life decreased slightly in both surgically and non-surgically treated groups one year after bony Bankart injury.
Conclusion: This national register-based study provides detailed information on the epidemiology, choice of treatment and patient-reported outcomes in a large cohort of bony Bankart lesions. Most bony Bankart lesions affected males between 40 and 75 years after low-energy falls and non-surgical treatment dominated.
Histone deacetylase inhibitors VPA and WT161 ameliorate the pathological features and cognitive impairments of the Alzheimer's disease mouse model by regulating the expression of App secretases
Authors: Zhang Miaomiao , Wang Wanyao , Ye Qun , Fu Yun , Li Xuemin , Yang Ke , Gao Fan , Zhou An , Wei Yonghui , Li Shen , Wei Fengjiang , Shi Wentao , Li Wei-Dong 
Published: July 2023

Abstract
Background
Alzheimer's disease (AD) is a degenerative neurological disorder. Recent studies have indicated that histone deacetylases (HDACs) are among the most prominent epigenetic therapy targets and that HDAC inhibitors have therapeutic effects on AD. Here, we identified sodium valproate (VPA), a pan-HDAC inhibitor, and WT161, a novel HDAC6 selective inhibitor, as potential therapeutic agents for AD. Underlying molecular mechanisms were investigated.
Methods
A cellular model, N2a-APPswe, was established via lentiviral infection, and the APPswe/PSEN1dE9 transgenic mouse model was employed in the study. Western blotting, immunohistochemical staining, thioflavin-S staining, and ELISA were applied to detect protein expression in cells, tissues, or serum. RNA interference was utilized to knockdown the expression of specific genes in cells. The cognitive function of mice was assessed via the nest-building test, novel object recognition test, and Morris water maze test.
Results
Previous studies have focused mainly on the impact of HDAC inhibitors on histone deacetylase activity. Our study discovered that VPA and WT161 can downregulate the expression of multiple HDACs, such as HDAC1 and HDAC6, in both AD cell and mouse models. Moreover, they also affect the expression of APP and APP secretases (BACE1, PSEN1, ADAM10). RNA interference and subsequent vitamin C induction further confirmed that the expression of APP and APP secretases is indeed regulated by HDAC1 and HDAC6, with the JNK pathway being the intermediate link in this regulatory process. Through the above pathways, VPA and WT161 effectively reduced Aβ deposition in both AD cell and mouse models and significantly improved cognitive function in AD mice.
Conclusions
In general, we have discovered that the HDAC6-JNK-APP secretases cascade is an important pathway for VPA and WT161 to exert their therapeutic effects on AD. Investigations into the safety and efficacy of VPA and WT161 were also conducted, providing essential preclinical evidence for assessing these two epigenetic drugs for the treatment of AD.
Trends in retail sales of insecticide-treated nets and untreated nets in Tanzania: cross-section surveys
Authors: Kamala Benjamin , Mwingizi Deo , Dadi David , Loll Dana , Gitanya Peter , Mwalimu Charles , Chacky Frank , Kajange Stella , Malima Sara , Khamis Mwinyi , Ibrahim Raya , Serbantez Naomi , Msangi Lulu , Koenker Hannah 
Published: July 2023

Abstract
Background
The commercial sector plays a vital role in mosquito net ownership and access in Tanzania. The National Malaria Strategic Plan (NMSP) includes long-lasting insecticide-treated nets (LLIN) delivery through the commercial sector as a complementary mechanism. The NMSP aims to increase LLIN sales while decreasing untreated mosquito net sales. This survey aimed to track quantities, market share of different net categories, prices, and origins of mosquito nets in retail markets and to engage stakeholders to analyze market trends.
Methods
This mixed-method mosquito net retail outlet survey was conducted in mid-2021 in six and in mid-2022 in eight regions. Field teams identified net-selling outlets in major urban and peri-urban markets and used snowball sampling to identify additional outlets. A structured questionnaire was used, and photos of available mosquito net products were taken. Key informant interviews were conducted with wholesalers and retailers. The relative market share of a product was calculated by using the mean of each sales category as frequency weights. Qualitative data analysis was undertaken by summarizing common themes and observations based on the research question.
Results
A total of 394 and 1,139 outlets were surveyed in 2021 and 2022, respectively. More than 96% of distributed brands in both years were untreated nets. The market share for untreated mosquito nets was 99.2% in 2021 and 88.3% in 2022. Bed net sales were seasonal, peaking in the rainy season and at the start of the school year. Leaked LLINs from the public sector comprised 0.3% of the market share in 2021 and 8.3% in 2022. Kigoma markets had the most significant frequency of leaked LLIN products. Legitimate LLINs were rare in 2021 (n = 2) and not found in 2022, despite the presence of a local LLIN manufacturer. A small number (n = 3) of untreated nets fabricated in China claiming to be LLINs were observed in 2022.
Conclusions
Despite NMCP's strategic approach to increasing retail market share for legitimate LLINs, significant challenges remain. The situation may be unlikely to change given the context of large-scale public sector distributions of LLINs, the higher consumer cost of LLINs, the lack of bed net varieties, and Tanzania’s relatively lengthy and expensive LLIN registration process.
Cascade testing in Italian Hereditary Breast Ovarian Cancer families: a missed opportunity for cancer prevention?
Authors: Trevisan Lucia , Godino Lea , Battistuzzi Linda , Innella Giovanni , Luppi Elena , Buzzatti Giulia , Gismondi Viviana , Blondeaux Eva , Bonelli Luigina Ada , Turchetti Daniela , Varesco Liliana 
Published: July 2023

Abstract
Healthy carriers of BRCA1/2 pathogenic variants (PVs) may benefit from risk-reducing measures of proven efficacy. The main approach to identify these individuals is cascade testing, and strategies to support this complex process are under investigation. In Italy, cascade testing has received little attention; therefore, we analyzed the uptake and characteristics of BRCA1/2 cascade testing in families diagnosed with HBOC at two Italian genetics centers between 2017 and 2019. All consanguineous family members who were older than 18 years of age at data collection time and who could be involved in the first step of cascade testing (i.e., all the living relatives closest to the proband) were included. In addition to first-degree relatives, individuals who were second-, third- or fourth-degree relatives were included if the closest relative(s) was/were deceased. Overall, 213 families were included (103, Genoa; 110, Bologna). Most probands were women affected by breast and/or ovarian cancer (86.4%, Genoa; 84.5%, Bologna), and the branch segregating the PV was known/suspected in most families (62.1%, Genoa; 60.9%, Bologna). Overall, the uptake of cascade testing was low (22%, Genoa; 16%, Bologna). It was associated with female gender (OR = 5.74, Genoa; OR = 3.33, Bologna), age < 30 years (OR = 10.31, Genoa; OR = 4.84, Bologna), first-degree relationship with the proband (OR = 20,94, Genoa; OR = 12.44, Bologna) and segregation of the PV in the paternal branch (OR = 2.90, Genoa; OR = 2.54, Bologna). These real-world data may be important to inform the design and implementation of strategies aimed at improving the uptake of HBOC cascade testing in Italy.
Study of fire behavior and evacuation in tunnel by simulation
Authors: Purwar Parth , Shukla Naman , Thanekar Arpit , A.B Bharath , Bansod Shail , Bhattacharya Shantanu , Singh D.K. 
Published: July 2023

Abstract
Over the past few decades, fire protection in tunnels is becoming very important as tunnels are gaining more popularity. It is constructed and used for transportation by people and freight all over the world. In order to achieve a fire safety purpose, evacuation systems should be well designed and built to evacuate people as early and safely as possible. The objective of this paper is to investigate the fire behavior and evacuation in a tunnel based on numerical simulation models in a longitudinally ventilated tunnel. In this study, a single fire caused by vehicles carrying different fire loads is been considered and simulated along with multiple varying conditions, where installation of Heating Ventilation and Air Conditioning (HVAC’s) and its capability of reversing the spread of smoke i.e., to prevent back layering is been checked. FDS simulation modelling tool was also majorly used to investigate the dangerous situations in which a fully loaded gasoline tank lorry was considered for burning simulation inside a long road tunnel. These simulation results were used to determine conditions which are faced by humans while evacuating any road tunnel that’s under fire. In this paper, multiple model-based experiments were carried out using tunnels of different sizes and ventilation conditions.
Spatiotemporal single cell analyses reveal a transient population of retinal progenitor cells in the ciliary margin of developing human retina
Authors: Lako Majlinda , Dorgau Birthe , Collin Joseph , Rozanska Agata , Zerti Darin , Crossier Moira , Hussain Rafiqul , Coxhead Jonathan , Dhanaseelan Tamil , Patel Aara , Sowden Jane , FitzPatrick David , Queen Rachel 
Published: July 2023

Abstract

The emergence of retinal progenitor cells (RPCs) and differentiation to various retinal cell types represent fundamental processes during retinal development. Herein, we provide a comprehensive single cell characterisation of transcriptional and chromatin accessibility changes that underline RPC specification and differentiation over the course of human retinal development up to midgestation. Our lineage trajectory data demonstrate the presence of early RPCs, which transit to late RPCs, and further to transient neurogenic progenitors, that give rise to all the retinal neurons. Combining single cell RNA-Seq with spatial transcriptomics of early eye samples, we demonstrate for the first time the transient presence of early RPCs in the ciliary margin zone with decreasing occurrence from 8 PCW of human development. In RPCs, we identified a significant enrichment for TEAD transcription factor binding motifs, which when inhibited led to loss of RPCs and retinal lamination, and inhibition of photoreceptor and retinal ganglion cell differentiation.