bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining Veterans’ Experiences With In Vitro Fertilization Provided Under P.L. 114–223
Authors: Mattocks Kristin M. , Marteeny Valerie , Walker Lorrie , Kroll-Desrosiers Aimee , Mengeling Michelle 
Published: January 2023

Background:
Until recently, the Department of Veterans Affairs (VA) medical benefits package has expressly excluded in vitro fertilization (IVF) services for Veterans experiencing fertility problems. However, P.L. 114–223 (2016) allows VHA to provide Assisted Reproductive Technology, including IVF, to certain eligible Veterans. Little is known regarding Veterans’ experiences accessing IVF through VA Community Care contracts with IVF providers.

Objective:
To examine Veterans’ experiences with IVF services provided under the auspices of P.L. 114–223.

Research Design:
Telephone or video interviews were conducted with male and female Veterans and opposite-sex spouses of Veterans who had been approved for IVF. Interviews were recorded, transcribed, and analyzed using content analysis techniques. Major themes and representative quotes were derived from the analyses.

Results:
Ninety-six Veterans and 14 spouses participated in our interviews. Six major themes arose from these interviews, including (1) the need for improved communication regarding IVF benefits, (2) the need for expanded IVF benefits, (3) the lack of a comprehensive care coordination program, (4) poor access to IVF providers in some areas of the country, (5) special services needed for Veterans with spinal cord injuries, and (6) the IVF policy may be discriminatory in nature to single and LGBTQ Veterans.

Conclusions:
Many Veterans with service-connected conditions related to reproductive health have taken advantage of the IVF benefit, though limitations on these benefits have prevented other Veterans from taking advantage of the IVF benefit. Further attention needs to be paid to improving communication and coordination of IVF services with ongoing VA care and ensuring special populations, including those living in rural areas and Veterans with spinal cord injuries, have access to IVF services as needed.
Successful use of differential target multiplexed spinal cord stimulation for chronic postsurgical abdominal pain
Authors: Tanaka Ryusuke , Shinohara Kenji , Hidai Yohei , Kiuchi Chiaki , Tanaka Satoshi , Kawamata Mikito , Sasao Junichi 
Published: January 2023

Abstract

Introduction:
Recent advances in stimulation techniques have improved the efficacy and expanded the applicability of spinal cord stimulation (SCS). Among these techniques, there are no reports on the efficacy of differential target multiplexed (DTM) SCS for chronic postsurgical pain (CPSP) after abdominal surgery. Therefore, we present the successful use of DTM SCS for CPSP after distal pancreatectomy.

Methods:
A 49-year-old man with hypertension and severe chronic low back pain presented with neuropathic CPSP involving the left abdomen in the area of a laparotomy incision. His pain was refractory to conservative treatment and was rated 10 on a numerical rating scale (NRS). He underwent permanent implantation of a pulse generator after a 14-day trial stimulation.

Results:
Chronic postsurgical pain was well controlled (NRS 1–2) at a 3-month follow-up with DTM SCS.

Conclusion:
Differential target multiplexed SCS can be a new treatment option for neuropathic CPSP that is resistant to conservative treatment. It is important to further examine the characteristics of CPSP and identify appropriate candidates for the successful use of DTM SCS.
Head and Neck Reconstruction With 2-Stage External Pectoralis Major Myocutaneous Flap Transfer
Authors: Moriguchi Kurumi , Kurita Tomoyuki , Fujii Takashi , Kawai Kenichiro , Kubo Tateki 
Published: January 2023

Background
In head and neck surgery cases where skin is severely scarred or adhered to surrounding tissue, reconstruction can be difficult to perform using microsurgical tissue or conventional pedicled pectoralis major myocutaneous flap (PMMF) transfer given the risks, which include damage to major vessels when manipulating scarred skin. For such cases, we perform a 2-stage external PMMF transfer, whereby the PMMF is directly sutured to the defect over the neck skin (without manipulation the hardened skin), and the flap division is then performed secondarily.

Methods
A total of 30 patients who had histories of radiotherapy (60–70 Gy) and prior neck dissection received the 2-stage external PMMF transfer procedure. Indications for the flaps included pharyngeal fistula closure in 12 patients, reconstruction for mandibular necrosis after radiotherapy in 8 patients, salvage surgery for partial or total necrosis of the free flap in 4 patients, and simultaneous reconstruction after tumor excision in 6 patients. Two patients underwent a second external PMMF transfer from the contralateral side because of postoperative complications, yielding a total of 32 flap transfers.

Results
Twenty-nine flaps showed complete flap survival, whereas 3 had partial necrosis. Other complications, despite complete flap survival, included large fistulas requiring additional surgery for fistula closure (1 patient) and small fistulas that were closed without requiring reoperation (7 patients).

Conclusions
External PMMF transfer offers a safe, simple, and effective option in cases where free flap surgery is considered difficult or the risks associated with neck manipulation are high.
Induction in Pancreas Transplantation: T-cell Depletion Versus IL-2 Receptor Blockade
Authors: Aziz Fahad , Parajuli Sandesh , Kaufman Dixon , Odorico Jon , Mandelbrot Didier 
Published: January 2023

Background.There is limited data exist on relative outcomes with T-depletion versus IL-2 receptor (IL2R) blockade induction in pancreas transplantation.Methods.We analyzed all patients who underwent simultaneous pancreas-kidney or pancreas transplant alone at our institution between January 1, 2011, and December 31, 2019.Results.Of 417 pancreas transplant recipients, 291 received induction with a T-depleting agent and 126 received induction with an IL2R blocker. No difference was detected in pancreas allograft death-censored (P = 0.7) or uncensored (P = 0.5) survival. Although pancreas rejection was more common overall (P = 0.03), this difference was no longer present in recipients at low immunologic risk (P = 0.08). Cytomegalovirus and bacterial infections were significantly more common in the patients who received T-cell depleting agents for induction (21% versus 11%,P = 0.03; 34% versus 23%,P = 0.04, respectively). On multivariate analysis, history of pancreas rejection (Hazard ratio (HR) = 4.7,P = 0.0001; 95% Confidence interval (CI), 2.16-10.12) and higher calculated panel reactive antibodies (HR = 1.01,P = 0.04; 95% CI, 1.0002-1.02) were associated with increased risk of pancreas allograft failure, but choice of induction was not (HR = 0.64,P = 0.3; 95% CI, 0.27-1.51). Further, on multivariate analysis, Cytomegalovirus infection was associated with increased risk of pancreas allograft rejection (HR = 1.78,P = 0.01; 95% CI, 1.11-2.87), but choice of induction was not (HR = 0.84,P = 0.46; 95% CI, 0.54-1.32). Similarly, bacterial infection was associated with increased risk of patient death (HR = 2.94,P = 0.04; 95% CI, 1.03-8.32).Conclusion.Our data suggest that IL-2 receptor blockade may be a reasonable choice of induction for pancreas transplant recipients at low immunologic risk.
The contribution of parental health to the subsequent social assistance entry of the family with children– a nationwide register-linked birth cohort study in Finland
Authors: Hiilamo Aapo , Keski-Säntti Markus , Räsänen Sami , Niemelä Mika , Lallukka Tea , Ristikari Tiina 
Published: January 2023

AimOur aim in this paper is to estimate the contribution of different parental specialised health care diagnoses to the subsequent risk of entry into the social assistance system for families with children in the period 1998–2013.MethodsWe used longitudinal population-level register data consisting of all children born in 1997 in Finland and their registered parents (54 960 one and two-parent families with 801 336 observations in the period 1998–2013). Diagnoses assigned in public specialised health care and social assistance records were derived from nationwide administrative registers. Measures of parental socioeconomic status and previous diagnoses and the birth weight of the child were adjusted for in regression models which estimated the association between parental diagnoses and entry into the social assistance system in the following year.ResultsFamilies with a parent somatic diagnosis had a risk ratio of 1.4 for social assistance entry in the subsequent year of the diagnosis though substantial variation by diagnosis category was detected. Parent psychiatric diagnoses were linked to a higher, 3.01-fold risk of social assistance entry. Covariate adjustment reduced these risk ratios to 1.2 and 2.1, respectively. Some 2.9 percent of all social assistance entries may be attributed to parental psychiatric diagnoses while somatic health records account for another 7.2 percent, making their total contribution over a tenth of all cases.ConclusionParental specialised health care records was associated with a higher risk of social assistance need as more interventions to support financial management are required for parents with psychiatric diagnoses.What is already known on this topicDisruptive life events, such as work disability, unemployment or divorce, are associated with social assistance claims and usage in the general populationWhat this study addsParents’ diagnoses, particularly when due to psychiatric conditions, are associated with a higher risk of entry into the social assistance systemHow this study might affect research, practice or policyThe findings are important in enabling us to recognise target groups and in designing interventions that aim to prevent families with children from falling into economic hardship
Cytokines in the Urine of AKI patients regulate TP53 and SIRT1 and can be used as biomarkers for the early detection of AKI
Authors: Erichsen Lars , Thimm Chantelle , Wruck Wasco , Kaierle Daniela , Schless Manon , Huthmann Laura , Dimski Thomas , Kindgen-Milles Detlef , Brandenburger Timo , Adjaye James 
Published: January 2023

AbstractAcute kidney injury (AKI) is a major kidney disease with a poor clinical outcome. It is a common complication with an incidence of 10-15% of patients admitted to hospital. This rate even increases for patients who are admitted to the intensive care unit with an incidence of >50%. AKI is characterized by a rapid increase in serum creatinine, decrease in urine output, or both. Associated Symptoms include feeling sick or being sick, diarrhoea, dehydration, decreased urine output-although occasionally urine output remains normal, fluid retention-causing swelling in the legs or ankles, shortness of breath, fatigue and nausea. However, sometimes acute kidney injury causes no signs or symptoms and is detected by lab tests. Therefore, an urgent demand for non-invasive biomarkers for early detection of AKI are highly desirable. This might enable the prevention of the progression from AKI to CKD.In this study, we analysed the secretome of urine of an AKI patient cohort employing a kidney-biomarker cytokine assay. Based on these results we suggest, ADIPOQ, EGF and SERPIN3A as potential biomarkers, which might be able to detect AKI as soon as 24 h post-surgery. For the later stages, common biomarkers for the detection of AKI in both male and female patients we suggest, VEGF, SERPIN3A, TNFSF12, ANPEP, CXCL1, REN, CLU and PLAU. These markers in combination might present a robust strategy to identify the development of AKI as early as 24h or 72h post-surgery. Furthermore, we evaluated the effect of patient and healthy urine on human podocyte cells. We conclude that cytokines in the urine of AKI patients trigger processes which are needed to repair the damaged nephron and activate TP53 and SIRT1 to maintain the balance between proliferation, angiogenesis, and cell cycle arrest. In conclusion, the Renin-Angiotensin pathway seems to have major implications.
Conflicts of Interest in Cardiology Journals
Authors: Smith- Manga Karsha M , Manga Atiba B , Piper Brian J 
Published: January 2023

AbstractBackgroundConflicts of interest are an ongoing concern in medical research. This takes place when the sponsor reports positive findings or promotes products over competitors because of their relationship and interaction with their industry sponsors. ThePhysicians Payment Sunshine Actmandates physicians who receive payments/compensation disclose their current and past relationships with different medical companies and the products they produce as an aim to manage conflicts of interest. In this quantitative bioethics study, we quantified financial conflicts of interest among cardiology journal authors and evaluated disparities in industry support among female and male physician-authors.MethodsWe reviewed 966 authors from 147 empirical research articles from two cardiology journals,Circulation, JCR 2021 Impact Factor (IF)= 29.7 andJournal of the American College of Cardiology(JACC), IF= 24.1, and one cardiovascular subspecialty journal,Catheterization and Cardiovascular Interventions(CCI), IF= 2.7. Articles published between January 1, 2020 and December 31, 2020 were reviewed. The database,OpenPayments.cms.gov(CMS-OP) was used to search author physician’s payments who reported receiving general payments, research payments, associated research funding, and ownership and investment interest between 2017 and 2019.ResultsA total of 19,529 payments totaled to 69,591,343.18 USD within the 36-month disclosure window. JACC accounted for 51.90%, Circulation 30.59% and CCI 17.51% of the total payment amounts. Male physician authors were more likely to receive industry sponsorship than their female counterparts (χ2(1)= 23.30;p<0.00001). The 139 male physicians with CMS-OP accounts made up 90.23% of payments, while females accounted for 9.77%.ConclusionIn conclusion, cardiology journal authors received appreciable renumeration form industry. Female cardiology authors had lower representation in authorship and honorarium compared to their male colleagues. There were also occasional author disclosure discrepancies, where some authors failed to report their relationship with financial institutions, despite each author receiving greater than one million dollars (USD) over a 36-month window. The evidence from this investigation supports that financial conflicts of interest is an ongoing issue in cardiology journals.
Effects of early life stress paired with adolescent alcohol consumption on two-bottle choice alcohol drinking behaviors in mice
Authors: Perry Thomas W. , Carvour Harrison M. , Reichert Amanda N. , Sneddon Elizabeth A. , Roemer Charlotte A.E.G. , Gao Ying Ying , Schuh Kristen M. , Shand Natalie A. , Quinn Jennifer J. , Radke Anna K. 
Published: January 2023

AbstractBackgroundIn humans, early life stress (ELS) is associated with an increased risk for developing both alcohol use disorder (AUD) and post-traumatic stress disorder (PTSD). We previously used an infant footshock model that produces stress-enhanced fear learning (SEFL) in rats and mice and increases aversion-resistant alcohol drinking in rats to explore this shared predisposition. The goal of the current study was to extend this model of comorbid PTSD and AUD to male and female C57BL/6J mice.MethodsAcute ELS was induced using 15 footshocks on postnatal day (PND) 17. In adulthood, alcohol drinking behavior was tested in one of three two-bottle choice drinking paradigms. In continuous access, mice were given 24 h access to 5% or 10% ethanol and water for five consecutive drinking sessions each. In limited access drinking in the dark, mice were given 2 h of access to 15% ethanol and water across 15 sessions 3 h into the dark cycle. In intermittent access, mice were presented with 20% ethanol and water Monday, Wednesday, and Friday, for four consecutive weeks. In a fifth week of intermittent access drinking, increasing concentrations of quinine (10 mg/L, 100 mg/L, and 200 mg/L) were added to the ethanol to test aversion-resistant drinking. Intermittent access drinking was tested with and without a period of adolescent drinking (PND 35).ResultsInfant footshock did not alter drinking in the continuous or limited access tasks. Adult consumption and preference were lower in the intermittent access task when adolescent drinking was included and there were ELS-induced differences in consumption of quinine-adulterated ethanol in females.ConclusionsOur results demonstrate that infant footshock followed by a period of adolescent drinking is a viable model of comorbid PTSD and AUD in rats and mice.
Role of Gut Microbiome in Neoadjuvant Chemotherapy Response in Urothelial Carcinoma: A Multi-Institutional Prospective Cohort Evaluation
Authors: Bukavina Laura , Ginwala Rashida , Sindhani Mohit , Prunty Megan , Geynisman Daniel , Pooja Ghatalia , Valentine Henkel , Calaway Adam , Brown Jason R. , Correa Andres , Mishra Kirtishri , Pominville Raymond , Plimack Elizabeth , Kutikov Alexander , Ghannoum Mahmoud , ElShaer Mohammed , Retuerto Mauricio , Uzzo Robert , Ponsky Lee , Abbosh Philip H. 
Published: January 2023

AbstractTreatment with neoadjuvant chemotherapy (NAC) in muscle invasive bladder cancer (MIBC) is associated with clinical benefit in urothelial carcinoma. While extensive research evaluating role of tumor mutational expression profiles and clinicopathologic factors into chemoresponse has been published, the role of gut microbiome (GM) in bladder cancer in chemoresponse has not been thoroughly evaluated. A working knowledge of the microbiome and its effect on all forms of cancer therapy in BC is critical. Here we examine gut microbiome of bladder cancer patients undergoing NAC. Overall, there was no significant difference in alpha and beta diversity by responder status. However, analysis of fecal microbiome samples showed that a higher abundance of Bacteroides within both institutional cohorts during NAC was associated with residual disease at the time of radical cystectomy regardless of chemotherapy regimen. Group community analysis revealed presence of favorable microbial subtypes in complete responders. Finally, fecal microbial composition outperformed clinical variables in prediction of complete response (AUC 0.88 vs AUC 0.50), however, no single microbial species could be regarded as a fully consistent biomarker. Microbiome-based community signature as compared to single microbial species is more likely to be associated as the link between bacterial composition and NAC response.
Gemini: Memory-efficient integration of hundreds of gene networks with high-order pooling
Authors: Woicik Addie , Zhang Mingxin , Xu Hanwen , Mostafavi Sara , Wang Sheng 
Published: January 2023

AbstractMotivationThe exponential growth of genomic sequencing data has created ever-expanding repositories of gene networks. Unsupervised network integration methods are critical to learn informative representations for each gene, which are later used as features for downstream applications. However, these network integration methods must bescalableto account for the increasing number of networks androbustto an uneven distribution of network types within hundreds of gene networks.ResultsTo address these needs, we present Gemini, a novel network integration method that uses memory-efficient high-order pooling to represent and weight each network according to its uniqueness. Gemini then mitigates the uneven distribution through mixing up existing networks to create many new networks. We find that Gemini leads to more than a 10% improvement in F1score, 14% improvement in micro-AUPRC, and 71% improvement in macro-AURPC for protein function prediction by integrating hundreds of networks from BioGRID, and that Gemini’s performance significantly improves when more networks are added to the input network collection, while the comparison approach’s performance deteriorates. Gemini thereby enables memory-efficient and informative network integration for large gene networks, and can be used to massively integrate and analyze networks in other domains.AvailabilityGemini can be accessed at:https://github.com/MinxZ/Gemini.Contactaddiewc@cs.washington.edu,swang@cs.washington.edu
Spectroscopic insights into the mechanism of anammox hydrazine synthase
Authors: Versantvoort Wouter , Hienerwadel Rainer , Ferousi Christina , van der Velden Pieter , Berthomieu Catherine , van Niftrik Laura , Baymann Frauke 
Published: January 2023

AbstractAnaerobic ammonium oxidizing bacteria make a living oxidizing ammonium with nitrite as electron acceptor, intermediates nitric oxide and hydrazine, and end product dinitrogen gas. Hydrazine is a biologically unique free intermediate in this metabolism, and is produced by the enzyme hydrazine synthase. Crystallization of ‘CandidatusKuenenia stuttgartiensis’ hydrazine synthase allowed for an initial hypothesis of its reaction mechanism. In this hypothesis, nitric oxide is first reduced to hydroxylamine after which hydroxylamine is condensed with ammonium to form hydrazine. Hydrazine synthase is a tetraheme cytochromec, containing two proposed active site hemes (γI & αI) in the γ- and α-subunit, respectively, connected by an intra-enzymatic tunnel. Here we combined the data from electrochemistry-induced Fourier transform infrared (FTIR) spectroscopy, EPR and optical spectroscopy to shed light on the redox properties and protein dynamics of hydrazine synthase in the context of its reaction mechanism. Redox titrations revealed two low potential low spin hemes with midpoint potentials of ∼-360 mV and ∼-310 mV for heme αII and γII, respectively. Heme γI showed redox transitions in the range of 0 mV, consisting of both low spin and high spin characteristics in optical and EPR spectroscopy. Electrochemistry-induced FTIR spectroscopy indicated an aspartic acid ligating a OH-/H2O at the heme γI axial site as a possible candidate for involvement in this mixed spin characteristic. Furthermore, EPR spectroscopy confirmed the ability of heme γI to bind NO in the reduced state. Heme αI exhibited a rhombic high spin signal, in line with its ligation by a proximal tyrosine observed in the crystal structure. Redox titrations down to −610 mV nor addition of dithionite resulted in the reduction of heme αI, indicating a very low midpoint potential for this heme.In vivochemistry at this heme αI, the candidate for the comproportionation of hydroxylamine and ammonium, is thus likely to be initiated solely on the oxidized heme, in contrast to previously reported DFT calculations. The reduction potentials of the γ-subunit hemes were in line with the proposed electron transfer of heme γII to heme γI for the reduction of NO to hydroxylamine (E0’ = − 30 mV).