bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the Morphology of Tamarix spp in the Mexicali Valley, Baja California, Mexico
Authors: Rodríguez-González Rosario E. , Sánchez-Castillo Marisol , Iñiguez-Monroy César G. , Soto-Ortíz Roberto , Rueda Puente Edgar O. , Brigido-Morales Juan G. 
Published: January 2025

Objective: The predominant species of Tamarix spp in the Mexicali Valley is unknown, and due to the scarce information available, this study aims to expand the knowledge of the morphology of Tamarix spp in the Mexicali Valley, Baja California, Mexico.
Design/methodology/approach: For this research, five branches with inflorescences and roots of Tamarix spp trees were collected from four selected locations in the Mexicali Valley. The collection was carried out during the flowering season from March to August, considering branches between 2.50 and 3.50 cm in height. The morphological descriptions were based on fresh plants using an Olympia optical microscope.
Results: After the morphological analysis was carried out at the different sampling sites, the predominant salt cedar genotype found in the Mexicali Valley corresponded to Tamarix chinensis. In addition, it was found high electrical conductivity measured in the upper soil layer (20 cm depth) was found to be caused by the excretion of salts through the glands of the leaves of this species. Consequently, salt cedar species can inhibit other vegetation types, although it can benefit honey bee production.
Findings/conclusions: Tamarix chinensis was the predominant salt cedar species throughout the sampling sites under the conditions of this study.The high electrical conductivity measured in the upper soil layer (20 cm depth) shows that salt cedar species can inhibit the growth of other vegetation types, although it can be beneficial for honey bee production.
Meningkatkan Hasil Belajar Peserta Didik melalui Model Problem Based Learning Berbasis Powtoon pada Materi Sistem Pernapasan
Authors: Filza Inne Aqmarina , Ningsih Kurnia , Yuniarti Anisyah , Titin Titin , Wahyuni Eko Sri 
Published: January 2025

Educators utilise learning models and media to improve students’ understanding of the material. This study aims to enhance students’ learning process and learning outcomes by using the Powtoon-based Problem Based Learning paradigm on respiratory system information. This research is a Classroom Action Research with a two-cycle design. Each learning cycle includes planning, implementation, observation, and reflection stages. The research subjects were students of SMP Negeri 12 Sungai Raya class VIII B in the 2023/2024 school year, with a sample size of 29 people. The instruments of this study were observation sheets and learning outcome tests. The purpose of the test sheet is to measure students’ understanding of the material discussed in class, while the observation sheet sees how well the learning objectives are achieved. The data collection strategies used in this study were tests, observations, and documentation. The results of the learning implementation process were obtained at 92.26% in cycle I and 95.64% in cycle II. Student learning outcomes increased by 86.20% in cycle II from 77.59% in cycle I. Therefore, it can be concluded that learning using the Powtoon-based Problem Based Learning model can improve the learning implementation process and student learning outcomes on respiratory system material.
Struktur Komunitas dan Status Konservasi Ikan Berdasarkan Alat Tangkap Tradisional di Desa Simonis Kabupaten Labuhan Batu Utara
Authors: Ritonga Irna Alfaini , Khairul Khairul , Harahap Hasmi Syahputra 
Published: January 2025

Fish diversity must be maintained because it has an ecological role, namely maintaining the balance of the ecosystem and can be used as an indicator of pollution. diversity is also important for the balance of aquatic ecosystems and some simonis villagers make it a community livelihood. Based on information from residents of Simonis Village, North Labuhan Batu Regency, it is recognized that the diversity found in the river is quite high. In addition, residents of simonis village, north labuhan batu district can also utilize river water in pulo biski as drinking water, washing clothes, bathing and tourism. This study aims to determine the community structure, ecological index and conservation status of fish obtained in Simonis Village based on IUCN data. This research is a survey with the determination of observation stations through purposive sampling method. Fish sampling was carried out using environmentally friendly fishing gear such as nets, shoots, fishing rods and tanggok or nets. The results of this study obtained approximately 22 species of fish and each fish species and morphological identification was carried out in order to know the scientific name of each type of fish. Index value and Diversity with moderate category. Diversity Index value with high category. Dominance Index value with no dominance criteria. It is concluded that the diversity of fish in Simonis Village, North Port Batu Regency.
Description of Students' Science Literacy Ability on Electrolyte and Non-Electrolyte Solution Materials at SMAN 11 Pontianak
Authors: Puspita Ninda Ikke , Junanto Tulus , Harun Andi Ifriany , Enawaty Eny , Ulfah Maria 
Published: January 2025

It is important for students to learn scientific literacy because it’s related to how students understand the environment and face issues in modern society. Students with excellent scientific literacy skills are able to apply the concepts and facts learned at school in everyday situations. The purpose of this research is to provide an overview of students’ scientific literacy at SMA Negeri 11 Pontianak. The research method used is descriptive research with a quantitative approach. The population under study consists of grade XI science students, including XI IPA 1, XI IPA 2, and XI IPA 3. Sample selection employs purposive sampling technique, considering specific factors such as students who have received instruction from teachers in the subject of electrolyte solutions and possess a good academic abilities. A total of 37 students from class XI IPA 2 are chosen as research samples. Data collection technique involves measurement and communications, utilizing instruments in the form of five essay questions that have been tested for validity. Based on the research data, it is known that aspect of explaining scientific phenomena reached 60,47%, while in determine the properties and samples of solutions from electrical conductivity experimental data, percentage is 75%. To provide conclusions and opnions on electrolyte test results from everyday life phenomena reached 61,93%. If averaged, overall, students’ scientific literacy skills are in the moderate category, with an average score of 65,251%.
The Relationship Between Metacognitive Ability and Self-directed Learning of Chemistry Education Students as Prospective Teacher
Authors: Candrawinata Natalia , Erlina Erlina , Ulfah Maria 
Published: January 2025

Metacognitive ability and self-directed learning are crucial aspects to consider because good metacognitive skills are closely linked to academic success and self-directed learning. Students with strong metacognitive abilities are generally more capable of planning, monitoring, and evaluating their learning activities effectively. This study utilized a descriptive quantitative method with a correlational approach to examine the relationship between two variables: metacognitive ability and self-directed learning. The research was conducted in the Chemistry Education Program at FKIP Untan, focusing on 51 sixth-semester students for the academic year 2023/2024. Data were collected through indirect communication methods using the MAIT-18 and a self-directed learning questionnaire. The data analysis used descriptive statistical methods. The results indicated that the percentages of metacognitive ability and self-directed learning among sixth-semester Chemistry Education students were 79% and 68%, respectively. The Spearman Rank correlation test revealed a significance value of 0.478 > 0.05. Therefore, H0 is accepted and Ha is rejected, indicating that there is no significant relationship between metacognitive ability and self-directed learning among the students of the Chemistry Education Program at FKIP Untan.
Analysis of The Ionic Bond and Related Concept in High School Chemistry Textbooks in Pekanbaru
Authors: Ardiansyah Ardiansyah , Fitriyani Indah 
Published: January 2025

Misconceptions about ionic bonding bond and related concept have been revealed by many studies. One source of student misconceptions is high school chemistry textbooks. This research aims to analyze the ionic bonding conceptions contained in Class X high school chemistry textbooks in the city of Pekanbaru. This research uses document analysis techniques. The research sample was 5 high school chemistry books for class X in Pekanbaru City. Misconception are grouped according to Hershey (2004), namely Oversimplifications, Overgeneralizations, Obsolete Concepts and Terms, Misidentifications, and Flawed Research. Most textbooks suffer from Overgeneralizations of the concepts of chemical stability, ionic bonds, and properties of ionic compound.
Validity of Salt Hydrolysis E-Module Based on PBL with Teaching at the Right Level Approach
Authors: Parhusip Efriani Br , Iryani Iryani 
Published: January 2025

The Independent Curriculum is an innovation introduced by the Ministry of Education, Indonesia, under regulation No. 56/M/2022. This curriculum concentrates on the core subjects while giving special attention to character development and flexible learning that is adjusted to meet the requirements of individual pupils. One of the chemistry topics in the Independent Phase F Curriculum is salt hydrolysis. The purpose was to determine the level of validation of the PBL-based salt hydrolysis e-module using the TaRL approach. This research employs the Plomp model as part of the educational design research (EDR) framework, encompassing the stages of preliminary research, prototyping, and evaluation, but is limited to Prototype III. This study used five validators to evaluate the module based on language, presentation, content, graphics, and media components using a validation questionnaire. The results obtained from this study are PBL-based salt hydrolysis e-modules with a valid TaRL approach, with a validation value of the content component of 0.92, presentation of 0.91, language of 0.97, graphics of 0.95, and media average of 0.90.
Global insights into the genome dynamics of Clostridioides difficile associated with antimicrobial resistance, virulence, and genomic adaptations among clonal lineages
Authors: Sholeh Mohammad , Beig Masoumeh , Kouhsari Ebrahim , Rohani Mahdi , Katouli Mohammad , Badmasti Farzad 
Published: January 2025

BackgroundClostridioides difficile is a significant cause of healthcare-associated infections, with rising antimicrobial resistance complicating treatment. This study offers a genomic analysis of C. difficile, focusing on sequence types (STs), global distribution, antibiotic resistance genes, and virulence factors in its chromosomal and plasmid DNA.MethodsA total of 19,711 C. difficile genomes were retrieved from GenBank. Prokka was used for genome annotation, and multi-locus sequence typing (MLST) identified STs. Pan-genome analysis with Roary identified core and accessory genes. Antibiotic resistance genes, virulence factors, and toxins were detected using the CARD and VFDB databases, and the ABRicate software. Statistical analyses and visualizations were performed in R.ResultsAmong 366 identified STs, ST1 (1,326 isolates), ST2 (1,141), ST11 (893), and ST42 (763) were predominant. Trends of genome streamlining included reductions in chromosomal length, gene count, protein-coding genes, and pseudogenes. Common antibiotic resistance genes—cdeA (99.46%), cplR (99.63%), and nimB (99.67%)—were nearly ubiquitous. Rare resistance genes like blaCTX-M-2, cfxA3, and blaZ appeared in only 0.005% of genomes. Vancomycin susceptibility-reducing vanG cluster genes were detected at low frequencies. Virulence factors showed variability, with highly prevalent genes such as zmp1 (99.62%), groEL (99.60%), and rpoB/rpoB2 (99.60%). Moderately distributed genes included cwp66 (54.61%) and slpA (79.02%). Toxin genes tcdE (91.26%), tcdC (89.67%), and tcdB (89.06%) were widespread, while binary toxin genes cdtA (26.19%) and cdtB (26.26%) were less common. Toxin gene prevalence, particularly tcdA and tcdB, showed a gradual decline over time, with sharper reductions for cdtA and cdtB. Gene presence patterns (GPP-1) for resistance, virulence, and toxin genes were primarily linked to ST2, ST42, and ST8.ConclusionThis study highlights C. difficile’s adaptability and genetic diversity. The decline in toxin genes reflects fewer toxigenic isolates, but the bacterium’s increasing preserved resistance factors and virulence genes enable its rapid evolution. ST2, ST42, and ST8 dominate globally, emphasizing the need for ongoing monitoring.
Feasibility of the area reduction post-closure technique for bedside weaning of veno-arterial extracorporeal membrane oxygenation
Authors: Xu Chen , Xu Guo-xiong , Cao Yi-fei , Chen Lei , Jin Yi-qi 
Published: January 2025

ObjectiveTo evaluate the safety and efficacy of the area reduction post-closure technique for bedside weaning of veno-arterial extracorporeal membrane oxygenation (V-A ECMO).MethodsA retrospective study was conducted from December 2022 to November 2023, analyzing data from patients who underwent V-A ECMO weaning at our center. The area reduction post-closure technique, utilizing two ProGlide devices (Abbott Vascular, Santa Clara, CA), was adopted as a standard practice. The technical success was defined as achieving complete hemostasis without a bailout open repair. The complications associated with access included hemorrhagic events, pseudoaneurysm formation, limb ischemia, distal embolization, and wound infections.ResultsA total of 18 patients were included. The median age of the cohort was 72.0 years [interquartile range (IQR), 57.5–81.5 years], with a male-to-female ratio of 2:1. The median size of arterial sheath utilized was 18.0 Fr (IQR, 17.0–20.0 Fr). The median duration of the procedure was 10.0 min (IQR, 9.0–13.0 min), and the median length of total hospital stay was 31.0 days (IQR, 25.5–39.0 days). Furthermore, the technique demonstrated a success rate of 100%. One patient (5.6%) experienced minor bleeding, which was successfully managed through compression. No additional complications associated with access were observed after the procedure.ConclusionsThe post-closure area reduction technique emerges as a viable option for bedside weaning of V-A ECMO. Nonetheless, it is essential that this technique be validated through larger comparative studies.
No improvement in pregnancy and perinatal outcomes with combined luteal support in modified natural cycle frozen embryo transfer
Authors: Zhang Wen , Wu Sheling , Ren Bingnan , Jia Ruolin , Zhang Wenjuan , Wang Bijun , Du Xiaofang , Guan Yichun 
Published: January 2025

ObjectiveWe investigated whether the addition of a luteal phase support drug benefits pregnancy and perinatal outcomes in modified natural-cycle frozen-thawed embryo transfer (mNC-FET) for women up to the age of 35 years.MethodsWe analyzed the clinical data of 3658 mNC-FET cycles of women up to the age of 35 years from the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University from January 2018 to December 2020 in a retrospective cohort study. The cycles were divided into three groups based on the luteal phase support protocol used. The patients in group A received a combination of progesterone soft capsules and dydrogesterone (882 cycles), those in group B received dydrogesterone only (627 cycles), and those in group C received a combination of progesterone vaginal sustained-release gel and dydrogesterone (2149 cycles). Pregnancy and perinatal outcomes were compared among the three groups.ResultsLogistic regression analysis indicated that the three luteal phase support regimens were not associated with the live birth rate [OR(95% CI)B vs A=1.080, p=0.960; OR(95% CI)B vs C=0.252, p=0.291]. There were no significant differences in the newborn weight, premature delivery rate, pregnancy complications rate, and incidence of birth defects among the three groups.ConclusionsIn the mNC-FET cycle, patients under the age of 35 who chose dydrogesterone alone as a luteal phase support drug exhibited no difference in the live birth rate and perinatal outcome from patients who combined dydrogesterone with progesterone soft capsules or with progesterone vaginal sustained-release gel. However, the outcome still requires confirmation by large-sample prospective studies.
Trends, seasonal variations and forecasting of chronic respiratory disease morbidity in charcoal producing areas, northwest Ethiopia: time series analysis
Authors: Tesfa Mulugeta , Motbainor Achenef , Yenesew Muluken Azage 
Published: January 2025

ObjectiveThis study analyzed the trend, seasonal variations and forecasting of chronic respiratory disease morbidity in charcoal producing areas, northwest Ethiopia, aiming to provide evidences in planning, designing strategies, and decision-makings for preparedness and resource allocation to prevent CRD and reduce public health burden in the future.Materials and methodsThe trend, seasonal variation, and forecasting for CRD were estimated using data collected from the three zones of Amhara region annual reports of DHIS2 records. Smoothing decomposition analysis was employed to demonstrate the trend and seasonal component of CRD. The ARIMA (2, 1, 2) (0, 0, 0) model was used to forecast CRD morbidity. The model's fitness was checked based on Bayesian information criteria. The stationarity of the data was assessed with a line chart and statistically with the Ljung-Box Q-test. SPSS version 27 was utilized for statistical analysis.ResultsThe annual morbidity rate of CRD has shown an increasing trend in both sexes over a seven-year period among people aged 15 years and older. Seasonal variation in CRD morbidity was observed. The smoothing decomposition analysis depicted that the seasonal component was attributed to 44.47% and 19.16% of excess CRD cases in the period between September to November, and June to August, respectively. A substantial difference among the three zones of the Amhara region in CRD morbidity rate was noted, with the highest observed in the Awi zone. Forecasting with the ARIMA model revealed that CRD-related morbidity will continue to increase from 2020 to 2030.ConclusionThe study revealed that the CRD morbidity rate has shown an increasing trend from 2013 to 2019. Seasonal variation in the CRD morbidity rate was observed, with the highest peak from September to November. The morbidity attributed to CRD will continue to increase for the next ten years (2020–2030). Therefore, this study could potentially play a groundbreaking role. Further study is warranted to understand the risk factors and facility readiness through a further understanding of seasonality and future trends.
Case Report: Novel ADA2 variants cause atypical adenosine deaminase 2 deficiency
Authors: Yu Haishao , Lin Shuangzhu , Li Lin , Li Jiayi , Chen Qiandui , Wu Yuheng , Qi Yangfan , Wang Wanqi , Chang Xingzhi , Zhang Jie 
Published: January 2025

Case presentationA girl aged 2 years and 5 months presented to the hospital with chief complaints of intermittent fever and weakness of the left limb for more than 1 month. The child had transient urticaria appearing on her face for 5 days. The inflammatory biomarkers were significantly increased. Brain MRI showed multiple ischemic lesions in the brain’s small vessels. The patient exhibited significant systemic inflammation and multiple vasculitis. Whole-exome sequencing showed c.1358A&gt;G p. (Tyr453Cys) and c.1082-7T&gt;A compound heterozygous variants in the adenosine deaminase 2 (ADA2) gene, of which the c.1082-7T&gt;A variant has not been reported yet in previous literature. Peripheral blood mRNA reverse transcription-Sanger sequencing confirmed that this variant affected mRNA splicing, resulting in a frameshift with premature stop codon c.1083_1103del p. (Leu362Glnfs*45). Peripheral blood test suggested a significant decrease in ADA2 activity. Eventually, the patient was diagnosed with deficiency of adenosine deaminase 2 (DADA2). Her condition improved after treatment with etanercept. She had no more fevers, and no hemiplegia attacks were observed during the 3 years of follow-up.ConclusionFever and hemiplegia were the main manifestations in this patient, without typical rashes. DADA2 was finally confirmed by enzymology and genetic testing, and we believe this is the first reported case of the c.1082-7T&gt;A intronic variant in DADA2, and the RNA studies conducted in this case have been pivotal in assessing its pathogenicity.
Classical and machine learning tools for identifying yellow-seeded Brassica napus by fusion of hyperspectral features
Authors: Liu Fan , Wang Fang , Zhang Zaiqi , Cao Liang , Wu Jinran , Wang You-Gan 
Published: January 2025

IntroductionDue to its favorable traits-such as lower lignin content, higher oil concentration, and increased protein levels-the genetic improvement of yellow-seeded rapeseed has attracted more attention than other rapeseed color variations. Traditionally, yellow-seeded rapeseed has been identified visually, but the complex variability in the seed coat color of Brassica napus has made manual identification challenging and often inaccurate. Another method, using the RGB color system, is frequently employed but is sensitive to photographic conditions, including lighting and camera settings.MethodsWe present four data-driven models to identify yellow-seeded B. napus using hyperspectral features combined with simple yet intelligent techniques. One model employs partial least squares regression (PLSR) to predict the R, G, and B color channels, effectively distinguishing yellow-seeded varieties from others according to globally accepted yellow-seed classification protocols. Another model uses logistic regression (Logit-R) to produce a probability-based assessment of yellow-seeded status. Additionally, we implement two intelligent models, random forest and support vector classifier to evaluate features selected through lasso-penalized logistic regression.Results and DiscussionOur findings indicate significant recognition accuracies of 96.55% and 98% for the PLSR and Logit-R models, respectively, aligning closely with the accuracy of previous methods. This approach represents a meaningful advancement in identifying yellow-seeded rapeseed, with high recognition accuracy demonstrating the practical applicability of these models.