bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic investigation of neural stem cell to oligodendrocyte precursor cell differentiation reveals phosphorylation-dependent Dclk1 processing
Authors: Hardt Robert , Dehghani Alireza , Schoor Carmen , Gödderz Markus , Cengiz Winter Nur , Ahmadi Shiva , Sharma Ramesh , Schork Karin , Eisenacher Martin , Gieselmann Volkmar , Winter Dominic 
Published: August 2023

AbstractOligodendrocytes are generated via a two-step mechanism from pluripotent neural stem cells (NSCs): after differentiation of NSCs to oligodendrocyte precursor/NG2 cells (OPCs), they further develop into mature oligodendrocytes. The first step of this differentiation process is only incompletely understood. In this study, we utilized the neurosphere assay to investigate NSC to OPC differentiation in a time course-dependent manner by mass spectrometry-based (phospho-) proteomics. We identify doublecortin-like kinase 1 (Dclk1) as one of the most prominently regulated proteins in both datasets, and show that it undergoes a gradual transition between its short/long isoform during NSC to OPC differentiation. This is regulated by phosphorylation of its SP-rich region, resulting in inhibition of proteolytic Dclk1 long cleavage, and therefore Dclk1 short generation. Through interactome analyses of different Dclk1 isoforms by proximity biotinylation, we characterize their individual putative interaction partners and substrates. All data are available via ProteomeXchange with identifier PXD040652.
Vermittlung der Terminservicestellen Psychotherapie im Jahr 2019
Authors: Engesser Deborah , Maier Lena , Schepers Markus , Singer Susanne 
Published: August 2023

Zusammenfassung
Hintergrund
Eine Maßnahme des Versorgungsstärkungsgesetzes war die Vermittlung psychotherapeutischer Leistungen (psychotherapeutische Sprechstunde, Akutbehandlung und probatorische Sitzungen) durch die Terminservicestellen der Kassenärztlichen Vereinigungen (KVen). Wir untersuchten diese Vermittlung differenziert nach Vermittlungsanliegen sowie nach Stadtstaat versus Flächenland.

Methoden
Die Anzahl der Anfragen und Terminvereinbarungen im Jahr 2019 wurde bei den KVen Deutschlands mittels Fragebogen erfragt. Die statistische Analyse bestand aus einer deskriptiven Auswertung und einem Kruskal-Wallis-Test.

Ergebnisse
Daten zu Terminvereinbarungen lagen von 17 KVen und Informationen zu Anfragen von 16 KVen vor. Insgesamt wurden 134.578 Termine vereinbart. Die Spannweite von 193 bis 21.810 Terminvereinbarungen offenbarte deutliche Unterschiede zwischen den einzelnen KVen. Der Anteil der psychotherapeutischen Sprechstunde an allen psychotherapeutischen Terminvereinbarungen lag im Median bei 92 %. Pro KV konnten im Median 87 % der Anfragen nach psychotherapeutischen Sprechstunden (Spannweite 56–100 %), 96 % nach Akutbehandlungen (29–100 %) und 97 % nach probatorischen Sitzungen (27–100 %) vermittelt werden (je n = 16). Es zeigten sich geringe Unterschiede zwischen Stadtstaaten und Flächenländern bei der Vermittlung von Akutbehandlungen und probatorischen Sitzungen.

Diskussion
Bei KVen sowohl in Stadtstaaten als auch in Flächenländern bestehen Defizite insbesondere bei der Vermittlung von Akutbehandlungen und probatorischen Sitzungen. Unsere Ergebnisse lassen keine Rückschlüsse auf den Vermittlungsradius oder die Wartezeit auf Termine zu.
Targeting ACC1 in T cells ameliorates psoriatic skin inflammation
Authors: Kao Yu-San , Mamareli Panagiota , Dhillon-LaBrooy Ayesha , Stüve Philipp , Godoy Gloria Janet , Velasquez Lis Noelia , Raker Verena Katharina , Weidenthaler-Barth Beate , Boukhallouk Fatima , Rampoldi Francesca , Berod Luciana , Sparwasser Tim 
Published: August 2023

Abstract
Psoriasis is a chronic inflammatory skin disease driven by the IL-23/IL-17 axis. It results from excessive activation of effector T cells, including T helper (Th) and cytotoxic T (Tc) cells, and is associated with dysfunctional regulatory T cells (Tregs). Acetyl-CoA carboxylase 1 (ACC1), a rate-limiting enzyme of fatty acid synthesis (FAS), directs cell fate decisions between Th17 and Tregs and thus could be a promising therapeutic target for psoriasis treatment. Here, we demonstrate that targeting ACC1 in T cells by genetic ablation ameliorates skin inflammation in an experimental model of psoriasis by limiting Th17, Tc17, Th1, and Tc1 cells in skin lesions and increasing the frequency of effector Tregs in skin-draining lymph nodes (LNs).

Key messages

ACC1 deficiency in T cells ameliorates psoriatic skin inflammation in mice.

ACC1 deficiency in T cells reduces IL-17A-producing Th17/Tc17/dysfunctional Treg populations in psoriatic lesions.

ACC1 deficiency in T cells restrains IFN-γ-producing Th1/Tc1 populations in psoriatic skin lesions and skin-draining LNs.

ACC1 deficiency promotes activated CD44+CD25+ Tregs and effector CD62L-CD44+ Tregs under homeostasis and psoriatic conditions.
Types and doses of anti‐adhesive agents injected into subacromial space do not have an effect on the clinical and anatomical outcomes after arthroscopic rotator cuff repair
Authors: Lee Jongwon , Yoon Jong Pil , Woo Youngje , Oh Kyung‐Soo , Chung Seok Won 
Published: August 2023

AbstractPurposeJoint stiffness after arthroscopic rotator cuff repair is a major concern for orthopaedic surgeons. Various antiadhesive agents are commonly administered after rotator cuff repair for its prevention. This study aimed to compare the outcomes among patients injected with different types and amounts of anti‐adhesive agents after rotator cuff repair. It was hypothesized that the outcomes might differ depending on the use of the anti‐adhesive agent and its type and dose.MethodsA total of 267 patients who underwent arthroscopic rotator cuff repair with or without subacromial injection of anti‐adhesive agents were enrolled. The first group (group A; 51 patients) were injected with 3 mL of poloxamer/sodium alginate‐based anti‐adhesive agent. The second group (group B; 93 patients) were injected with 3 mL of sodium hyaluronate‐based anti‐adhesive agent. The third group (group C; 82 patients) were injected with 1.5 mL of sodium hyaluronate‐based anti‐adhesive agent. Finally, the last group (group D; 41 patients) who did not use anti‐adhesive agents served as the control. The range of motion (ROM) and pain VAS scores were measured preoperatively and at 5 weeks, 3 months, 6 months, and 1 year postoperatively. Functional outcomes were evaluated using American Shoulder and Elbow Surgeons and Constant scores, whereas cuff integrity was assessed via MRI or ultrasonography at least 6 months postoperatively.ResultsAll ROM measurements, pain VAS scores, and functional scores were significantly improved regardless of the use, type, and dose of the anti‐adhesive agents. In addition shoulder ROM and rotator cuff healing did not significantly differ among the groups (all n.s.).ConclusionsNo significant differences were found in the clinical and anatomical outcomes according to the type and dose of the anti‐adhesive agents subacromially injected after rotator cuff repair.Level of evidenceIII.
The causal impact of mental health on tobacco and alcohol consumption: an instrumental variables approach
Authors: Mitrou Francis , Nguyen Ha Trong , Le Huong Thu , Zubrick Stephen R. 
Published: August 2023

AbstractThe reciprocal relationship between psychiatric and substance use disorders is well-known, yet it remains largely unknown whether mental health morbidity causally leads to addictive behaviours. This paper utilises a fixed effects instrumental variables model, which is identified by time-varying sources of plausibly exogenous variations in mental health, and a nationally representative panel dataset from Australia to present robust evidence on the causal impact of mental distress on cigarette smoking and alcohol drinking behaviours. We find that mental distress significantly increases the prevalence and intensity of either cigarette or alcohol consumption. Further analysis reveals that mental distress also substantially increases household monetary expenditures on either tobacco or alcohol. The impact is greater for lower educated individuals or children of smokers, and is slightly higher for males. Our findings highlight the importance of mental health screening and treatment programs, especially among lower educated individuals or children of smokers, to assist in the prevention of addictive activities.
Electron density analysis on the alpha acidity of nitriles
Authors: López José Luis , Teixeira Filipe , Graña Ana M. , Mosquera Ricardo A. 
Published: August 2023

Abstract24 substituted cyanocompounds and the corresponding anions obtained upon H+-abstraction from diverse positions were subjected to an electron density analysis with the quantum theory of atoms in molecules (QTAIM). All the electron densities were obtained at the B3LYP/6–31 +  + G(2d,2p) level on completely optimized geometries. In accordance to experimental evidence, α-H+abstraction is found as the most favored one (by at least 100 kJ mol−1in all the tested compounds). The presence of additional resonance electron attractors reduces significantly the α-deprotonation energy, whereas this magnitude is quite insensitive to the inclusion of resonance electron donors. The electron density rearrangement accompanying the deprotonation is apparently in line with the predictions of the resonance model (RM). In fact, a significant part of the electron density gained by expelling the proton is transferred to cyano N and to other groups where significant resonance structures delocalize the negative charge. Nevertheless, some significant modifications have to be introduced on the RM picture when the QTAIM results are studied in detail.
Clinical impact of Prostate-Specific Membrane Antigen Positron Emission Tomography (PET) on intensification or deintensification of advanced renal cell carcinoma management
Authors: Pathmanathan Shivanshan , Tariq Arsalan , Pearce Adam , Rhee Handoo , Kyle Samuel , Raveenthiran Sheliyan , Wong David , McBean Rhiannon , Marsh Phillip , Goodman Steven , Dhiantravan Nattakorn , Esler Rachel , Dunglison Nigel , Navaratnam Anojan , Yaxley John , Thomas Paul , Pattison David A. , Goh Jeffrey C. , Gan Chun Loo , Roberts Matthew J. 
Published: August 2023

Abstract
Purpose
There is an emerging role of the use of Prostate-Specific Membrane Antigen (PSMA) Positron Emission Tomography (PET) in renal cell carcinoma. Herein, we report our experience in use of PSMA PET in recurrent or metastatic renal cell carcinoma (RCC).

Methods
A retrospective analysis of all patients who underwent PSMA PET for suspected recurrent or de-novo metastatic RCC between 2015 and 2020 at three institutions was performed. The primary outcome was change in management (intensification or de-intensification) following PSMA PET scan. Secondary outcomes included histopathological correlation of PSMA avid sites, comparison of sites of disease on PSMA PET to diagnostic CT and time to systemic treatment.

Results
Endoscopic cadaveric analysis of the origin of the ophthalmic artery
Authors: Zhou Chunhui , Lei Ting , Sun Junzhao , Zhao Hulin , Yu Xin , Cao Weidong , Lv Wenying , Zhang Jianning 
Published: August 2023

Abstract
Purpose
The ophthalmic artery is often involved in suprasellar and parasellar surgeries, but the anatomical structure where the ophthalmic artery originates has not been fully clarified from the perspective of an endoscopic endonasal approach (EEA).

Methods
A total of 10 fresh cadaveric heads (20 sides) were dissected through an EEA, and the origin of the bilateral ophthalmic arteries and their adjacent structures were observed from a ventral view. The origin of the ophthalmic artery in 50 healthy people was retrospectively studied on computed tomography angiography imaging.

Results
The ophthalmic artery originated from the intradural segment (75%), paraclinoid segment (15%), or parasellar segment (10%) of the internal carotid artery. The cross-sectional view of the internal carotid artery through the EEA showed that the ophthalmic artery originated from the middle 1/3 (75%) or medial 1/3 (25%) of the upper surface of the internal carotid artery. On computed tomography angiography, the ophthalmic artery originated from the middle 1/3 (77%) and medial 1/3 (22%) of the upper surface of the internal carotid artery. All ophthalmic arteries were near the level of the distal dural ring (DDR) of the internal carotid artery, that is, within 3 mm above or below the DDR.

Conclusions
The ophthalmic artery usually originates in the middle 1/3 of the upper surface of the intradural segment of the internal carotid artery within 3 mm of the DDR. The ophthalmic artery can be protected to the utmost extent after its origin is identified through an EEA.
Step-in dosing of bosutinib in pts with chronic phase chronic myeloid leukemia (CML) after second-generation tyrosine kinase inhibitor (TKI) therapy: results of the Bosutinib Dose Optimization (BODO) Study
Authors: Isfort Susanne , Manz Kirsi , Teichmann Lino L. , Crysandt Martina , Burchert Andreas , Hochhaus Andreas , Saussele Susanne , Kiani Alexander , Göthert Joachim R. , Illmer Thomas , Schafhausen Philippe , Al-Ali Haifa Kathrin , Stegelmann Frank , Hänel Mathias , Pfeiffer Tim , Giagounidis Aristoteles , Franke Georg-Nikolaus , Koschmieder Steffen , Fabarius Alice , Ernst Thomas , Warnken-Uhlich Mareille , Wolber Uta , Kohn Denise , Pfirrmann Markus , Wolf Dominik , Brümmendorf Tim H. ,   for the German CML study group
Published: August 2023

AbstractThe approved dose of bosutinib in chronic phase CML is 400 mg QD in first-line and 500 mg QD in later-line treatment. However, given that gastrointestinal (GI) toxicity typically occurs early after treatment initiation, physicians often tend to start therapy with lower doses although this has never been tested systematically in prospective trials in the Western world. The Bosutinib Dose Optimization (BODO) Study, a multicenter phase II study, investigated the tolerability and efficacy of a step-in dosing concept of bosutinib (starting at 300 mg QD) in chronic phase CML patients in 2nd or 3rd line who were intolerant and/or refractory to previous TKI treatment. Of 57 patients included until premature closure of the study due to slow recruitment, 34 (60%) reached the targeted dose level of 500 mg QD following the 2-weekly step-in dosing regimen. While the dosing-in concept failed to reduce GI toxicity (grade II–IV, primary study endpoint) to < 40% (overall rate of 60%; 95% CI: 45–74%), bosutinib treatment (mean dosage: 403 mg/day) showed remarkable efficacy with a cumulative major molecular remission (MMR) rate of 79% (95% CI: 66 to 88%) at month 24. Of thirty patients refractory to previous therapy and not in MMR at baseline, 19 (64%) achieved an MMR during treatment. GI toxicity did not significantly impact on patient-reported outcomes (PRO) and led to treatment discontinuation in only one patient. Overall, the results of our trial support the efficacy and safety of bosutinib after failure of second-generation TKI pre-treatment. Trial registration: NCT02577926.
Artificial cranial deformation in Tiwanaku, Bolivia
Authors: Narang Pranay , Jandial Zain , Aramayo Jorge Daniel Brun , Crawford John , Levy Michael L. 
Published: August 2023

Abstract
Purpose
Herein lies a brief historical review of the practice of artificial cranial deformation (ACD) in Tiwanaku, Bolivia, a pre-Columbian archeological ruin once regarded as one of the most powerful pre-Inca regions whose influence extended into present-day Peru and Chile from 600 to 1000 AD. We describe the history, purpose, and implications of ACD from both a neuroanatomical and cultural perspective.

Methods
A literature review was conducted through PubMed on the history of artificial cranial deformation in South America, concentrating on the Tiwanaku region. The authors searched all available data with no specific time reference, using the mentioned keywords: ACD, neuroanatomical implications of ACD, cultural and social functions of ACD, Tiwanaku society, and Andean civilization.

Results
Early Andean civilization was hierarchical and stratified. In Tiwanaku, the practice of ACD served to delineate one’s social class, caste, lineage, and vocation. This was especially useful for warriors, who distinguished their fellow combatants from insurgents by differences in their cranial structure. ACD was usually conducted within the first few months of an infant’s life before morphogenetic features became permanent. Two popular cranial styles—tabular and annular—were achieved by applying various mechanical apparatus and resulted in several cranial shapes (conical, box-like, flattened, etc.). Neuroanatomically, each deformation technique and the duration for which mechanical stress was applied influenced the solidification of cranial bones and shaped the frontal, occipital, parietal, and temporal bones differently. Cognitive deficits and plagiocephalic defects were recorded in limitation and may have been overlooked as the era’s occupational demands were more labor-intensive than knowledge-driven.

Conclusion
In Tiwanaku, the custom of ACD was used to demonstrate group identity, with alterations of the cranial shape corresponding to a particular headdress. ACD was used to distinguish an individual’s social identity, separating different groups of society into castes, classes, and slaves (Brain, 1979). The custom has also been used to mark territory and emphasize ethnic differences among groups, with potential cognitive implications that were largely unrecorded.
Efficacy of prostaglandin E2 versus prostaglandin F2 alpha assisted with narrowband-UVB in stable vitiligo
Authors: Neinaa Yomna Mazid El-Hamd , Mahmoud Myada Abd Elmohsen , El Maghraby Gamal Mohamed , Ibrahim Zeinab Abd Elsamd 
Published: August 2023

AbstractIn the recent decades, prostaglandins were recommended as a new therapeutic modality of stable vitiligo with promising efficacy. Therefore, we designed the current work to compare the significance of two different subtypes of prostaglandins [prostaglandin E2 (PGE2) versus prostaglandin F2 alpha (PGF2α)], assisted with NB-UVB phototherapy, in treatment of stable vitiligo. This study was conducted on 30 patients with stable non-segmental vitiligo. Three approximately similar vitiliginous areas were chosen in each patient and assigned into 3 groups. Each group treated with intradermal injection of either PGE2 (group I), PGF2α (group II), or saline as placebo (group III) at frequency once/week for 12 weeks. Concomitantly, all groups received NB-UVB phototherapy twice weekly for 3 months. The outcomes of this study discovered that the therapeutic efficacy of intradermal injection of either PGE2 or PGF2α assisted with NB-UVB phototherapy was comparable with non-significant difference between them in spite of being significantly higher than NB-UVB alone. However, there were a significantly earlier onset of repigmentation and higher degree of satisfaction regarding areas treated with PGE2 than those treated with PGF2α. In conclusion, both PGF2α and PGE2 intradermal injection could be considered as quite simple and affordable techniques in the treatment of stable vitiligo with no reported side effects and good patient satisfaction.
Lifetime ADHD symptoms highly prevalent in women with cardiovascular complaints. A cross-sectional study
Authors: ter Beek L. S. , Böhmer M. N. , Wittekoek M. E. , Kooij J. J. S. 
Published: August 2023

AbstractPatients with Attention Deficit Hyperactivity Disorder (ADHD) are at greater cardiovascular risk. We investigated the association between ADHD symptoms and cardiovascular disease in women at a specialized Dutch cardiological clinic. Lifetime ADHD symptoms were found in 35% of women (n = 300) with cardiac complaints. Women with ADHD symptoms compared to those without were significantly younger but had no different cardiological profile. To protect women’s health, further research and multidisciplinary cooperation is required to better understand the relationship between ADHD and cardiovascular disease.
Laparoscopic versus hybrid approach for treatment of incisional ventral hernia: a 5–10-year follow-up of the randomized controlled multicenter study
Authors: Hiekkaranta J. M. , Ahonen M. , Mäkäräinen E. , Saarnio J. , Pinta T. , Vironen J. , Niemeläinen S. , Vento P. , Nikki M. , Ohtonen P. , Rautio T. 
Published: August 2023

Abstract
Purpose
In this long-term follow-up of a prospective, randomized, and multicenter study, we compare the results of a group receiving laparoscopic incisional ventral hernia repair using intraperitoneal onlay mesh (LG) to a group receiving a hybrid hernia repair where open closure of fascial defect was added to intraperitoneal mesh placement (HG).

Methods
Originally, 193 patients with 2–7 cm incisional hernias were randomly assigned to either the LG or HG during the 30-month recruitment period in 2012 to 2015. Long-term follow-up was conducted 5–10 years after surgery to evaluate hernia recurrence rate and quality of life (QoL).

Results
In all, 65 patients in the LG and 60 in the HG completed the long-term follow-up with a median follow-up period of 87 months. Recurrent hernia was detected in 11 of 65 patients (16.9%) in the LG and 10 of 60 patients (16.7%) in the HG (p  >  0.9). Kaplan–Meier analysis demonstrated a recurrence rate approaching 20% in both groups, with similar curves. Three patients in the LG (4.6% and five patients in the HG (8.1%) had undergone re-operation due to recurrence (p  = 0.48). There was no difference in patient-reported QoL measured using the SF-36 questionnaire. Mean pain scores were similar between groups, mean numeric rating scale (NRS) 0 to 10 being 1.1 in the LG and 0.7 in the HG (p  = 0.43).

Conclusion
Fascial closure did not reduce hernia recurrence rate in this study population, even though it has been shown to be beneficial and recommended in surgery guidelines. In the long term, recurrence rate for both groups is similar.
A manifesto for applicable formal methods
Authors: Gleirscher Mario , van de Pol Jaco , Woodcock Jim 
Published: August 2023

AbstractRecently, formal methods have been used in large industrial organisations (including AWS, Facebook/Meta, and Microsoft) and have proved to be an effective part of a software engineering process finding important bugs. Perhaps because of that, practitioners are interested in using them more often. Nevertheless, formal methods are far less applied than expected, particularly for safety-critical systems where they are strongly recommended and have the most significant potential. We hypothesise that formal methods still seem not applicable enough or ready for their intended use in such areas. In critical software engineering, what do we mean when we speak of a formal method? And what does it mean for such a method to be applicable both from a scientific and practical viewpoint? Based on what the literature tells about the first question, with this manifesto, we identify key challenges and lay out a set of guiding principles that, when followed by a formal method, give rise to its mature applicability in a given scope. Rather than exercising criticism of past developments, this manifesto strives to foster increased use of formal methods in any appropriate context to the maximum benefit.