bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ‐DTL sponges miR‐758‐3p to accelerate cervical cancer malignant progression by regulating DCUN1D1 expression
Authors: Luo Xiaoning , Liu Jiewen , Wang Xiangcai , Yuan Jun , Zhang Yu 
Published: July 2023

AbstractCircular RNAs (circRNAs) play important roles in regulating various cancer progression. However, the function and clinical significance of circ‐denticleless E3 ubiquitin proteinligase homolog (DTL) in cervical cancer (CC) have not been studied. The present work explored the function and mechanism of circ‐DTL in CC development. Quantitative real‐time polymerase chain reaction (qRT‐PCR) was performed to examine the expression of circ‐DTL, miR‐758‐3p, and DCUN1D1. Cell Counting Kit‐8 (CCK‐8) and 5‐ethynyl‐2′‐deoxyuridine (EdU) assays were used to detect cell proliferation. Cell cycle and cell apoptosis were investigated by flow cytometry. Wound‐healing assay and transwell assay were conducted to assess cell migration and cell invasion. Western blot assay was carried out to determine protein expression. Dual‐luciferase reporter assay and RNA immunoprecipitation (RIP) assay were used to identify the relationship between miR‐758‐3p and circ‐DTL or DCUN1D1. Xenograft mouse model assay was conducted to explore the role of circ‐DTL in CC progression in vivo. Circ‐DTL and DCUN1D1 expression were upregulated in CC tissues and CC cells, but miR‐758‐3p expression was downregulated. Knockdown of circ‐DTL inhibited CC cell growth, migration, and invasion and promoted cell cycle arrest and cell apoptosis. Circ‐DTL could sponge miR‐758‐3p to modulate CC cell progression. Moreover, miR‐758‐3p inhibited CC malignant development by suppressing DCUN1D1 expression. In addition, circ‐DTL knockdown repressed CC cell tumor properties in vivo. Circ‐DTL acted as a tumor promoter in CC development by regulating the miR‐758‐3p/DCUN1D1 pathway.
Are we well? A post‐pandemic snapshot of dental educator wellness, well‐being, and fulfillment
Authors: Singh Amisha , Sarmiento Mónica , Truong Christina , Carrasco Gladys , Dugarte Alba , Quinn Barry , Panagakos Foti 
Published: July 2023

AbstractIntroductionThe COVID‐19 pandemic brought unprecedented changes to oral care and dental education delivery. To date, the vast majority of studies focus on the impact COVID‐19 had on the well‐being and wellness of patients, practitioners, and students; however, limited literature addresses the pandemic's impact on dental educators.PurposeThe aim of this study was to investigate the wellness, well‐being, and fulfillment of dental educators in the years following the onset of the COVID‐19 pandemic.MethodsFollowing Institutional Review Board approval, an anonymous electronic Qualtrics survey was emailed to full‐time and adjunct faculty across five (5) dental education institutions in the US. Faculty self‐reported on their wellness, general well‐being, physical well‐being, mental well‐being, fulfillment, and work–life balance. Group differences were examined using analysis of variance and the Tukey–Kramer test for multiple comparisons at the p < 0.05 level.ResultsThe results revealed females and younger dental faculty members reported statistically significantly lower levels of wellness (F2, 123 = 11.16, p < 0.001, F3, 121 = 8.53, p < 0.001), physical (F2, 123 = 11.53, p < 0.001, F3, 121 = 5.54, p = 0.001) and mental well‐being (F2, 123 = 12.49, p < 0.001, F3, 121 = 8.63, p < 0.001), fulfillment (F3, 121 = 5.01, p < 0.003), and higher levels of burnout (F2, 123 = 5.53, p = 0.005, F3, 121 = 4.85, p < 0.003). Those who expressed higher levels of burnout also had statistically lower mean well‐being scores (F4, 119 = 10.54, p < 0.001). Females also reported a significantly lower work–life balance score compared to male respondents (F2, 121 = 10.37, p < 0.002).ConclusionDespite the social and environmental challenges faced over the last couple of years post‐pandemic, dental educators demonstrate a quick ability to adapt, however, disproportional differences in gender and age groups were identified as being significantly correlated to dental educators’ self‐reports on wellness, well‐being, and fulfillment. Insight into these variables can help inform strategies in the academic setting to help support and strengthen the academic workforce.
Morphological and molecular changes in the Harderian gland of streptozotocin‐induced diabetic rats
Authors: Romano Maria Zelinda , Boccella Serena , Venditti Massimo , Maione Sabatino , Minucci Sergio 
Published: July 2023

AbstractUsing a rat model of type 1 diabetes (T1D) obtained by treatment with streptozotocin, an antibiotic that destroys pancreatic β‐cells, we evaluated the influence of subsequent hyperglycemia on the morphology and physiology of the Harderian gland (HG). HG is located in the medial corner of the orbit of many terrestrial vertebrates and, in rodents, is characterized by the presence of porphyrins, which being involved in the phototransduction, through photo‐oxidation, produce reactive oxygen species activating the autophagy pathway. The study focused on the expression of some morphological markers involved in cell junction formation (occludin, connexin‐43, and α‐tubulin) and mast cell number (MCN), as well as autophagic and apoptotic pathways. The expression of enzymes involved in steroidogenesis [steroidogenic acute regulatory protein (StAR), and 3β‐hydroxysteroid dehydrogenase (3β‐HSD)] and the level of lipid peroxidation by thiobarbituric acid reactive species assay were also evaluated. The results strongly indicate, for the first time, that T1D has a negative impact on the pathophysiology of rat HG, as evidenced by increased oxidative stress, morphological and biochemical alterations, hyperproduction and secretion of porphyrins, increased MCN, reduced protein levels of StAR and 3β‐HSD, and, finally, induced autophagy and apoptosis. All the combined data support the use of the rat HG as a suitable experimental model to elucidate the molecular damage/survival pathways elicited by stress conditions.
Multiomics analyses reveal pathological mechanisms of HBV infection and integration in liver cancer
Authors: Guo Mengbiao , Zhao Linghao , Jiang Chen , Jia Chang‐chang , Liu Hui , Zhou Weiping , Songyang Zhou , Xiong Yuanyan 
Published: July 2023

AbstractHepatitis B virus (HBV) infection and integration are important for hepatocellular carcinoma (HCC) initiation and progression, while disease mechanisms are still largely elusive. Here, we combined bulk and single‐cell sequencing technologies to tackle the disease mechanisms of HBV‐related HCC. We observed high HBV mutation rate and diversity only in tumors without HBV integration. We identified human somatic risk loci for HBV integration (VIMs). Transcription factors (TFs) enriched in VIMs were involved in DNA repair and androgen receptor (AR) signaling. Aberration of AR signaling was further observed by single‐cell regulon analysis in HBV‐infected hepatocytes, which showed remarkable interactions between AR and the complement system that, together with the X‐linked ZXDB regulon that contains albumin (ALB), probably contribute to HCC male predominance. Complement system dysregulation caused by HBV infection was further confirmed by analyses of single‐cell copy numbers and cell–cell communications. Finally, HBV infection‐associated immune cells presented critical defects, including TXNIP in T cells, TYROBP in NK cells, and the X‐linked TIMP1 in monocytes. We further experimentally validated our findings in multiple independent patient cohorts. Collectively, our work shed light on the pathogenesis of HBV‐related HCC and other liver diseases that affect billions of people worldwide.
Pharmacist ambulatory pain services for a chronic non‐cancer pain clinic: a descriptive study
Authors: Maleki Sam , Szmerling Jeremy , Thiruvasagan Mahisha , Seah Gloria , Gu Galahad 
Published: July 2023

AbstractBackgroundPatients presenting to specialist pain clinics are often prescribed complex and high‐risk analgesic regimens. In this setting, pharmacists can help identify at risk patients and provide valuable clinical input to improve patient outcomes.AimTo evaluate the role of a clinical pharmacist in improving the triage, assessment and management of patients presenting to ambulatory pain clinics.MethodThis descriptive study enrolled patients from two chronic noncancer pain clinics from July to December 2021. A new clinical pharmacist service was devised, which operated as telehealth one day per week. The pharmacist was responsible for accepting referrals, triaging based on clinic criteria and updating them with prescription risk factors, in line with SafeScript's traffic light system. Pharmacist clinical services included completion of medication histories, drug dose interventions and patient education. This project received departmental ethical approval from the Eastern Health Office of Research and Ethics prior to commencement (Approval No: QA21‐068).ResultsThe pharmacist assessed 253 patients and identified prescription risk factors for 68 patients; of which 11 (16%) had received opioids from ≥4 prescribers, 10 (15%) were issued a monitored prescription from ≥4 pharmacies, seven (10%) were on high‐risk drug combinations, 25 (37%) had an Oral Morphine Equivalent Daily Dose (OMEDD) of 50–100 mg, and 26 (38%) with OMEDD >100 mg. The pharmacist completed 67 medication histories and provided 22 accepted drug intervention recommendations to clinic physicians, including seven recommendations for dose adjustments, six to cease a medicine and nine to start a new medicine.ConclusionThe pharmacist incorporated SafeScript in the clinics' triage processes and provided valuable clinical interventions complementing the care provided by the pain physicians and the allied health staff.
Kinetics of bis(2‐methyl) butylene carbonate by transesterification of dimethyl carbonate with 1,4‐butanediol
Authors: Zhao Yanfang , Pei Tingting , Liang Rihui , Zhao Guoliang , Yang Zhongkai 
Published: July 2023

AbstractThe kinetic behavior of bis(2‐methyl) butylene carbonate (BMBC) by the transesterification of dimethyl carbonate (DMC) with 1,4‐butanediol (BDO) was investigated experimentally and theoretically. The Fourier transform infrared spectroscopy (FTIRs) test confirmed that the BMBC was successfully synthesized. The optimum preparation process of BMBC was investigated at atmospheric pressure, where Zn(Ac)2∙2H2O was the best catalyst for this transesterification reaction, and the optimal concentration was 0.3 wt%. The conversion was determined by measuring the amount of methanol produced during the reaction by refractometric method. A kinetic model was proposed according to the experimental results. The results showed that the transesterification reaction was a pseudo‐first‐order reaction. The apparent activation energy (Ea) significantly decreased with the increase of catalyst concentration from 0.1 wt% to 0.5 wt%. The Ea of the reaction was 102.13, 84.36, and 70.18 kJ mol−1, respectively, when the catalyst concentration was 0.1 wt%, 0.3 wt%, and 0.5 wt%. Furthermore, the parameters of the optimal heating curve in the batch reactor was obtained according to the optimal model.
Histologic Examination of Vocal Fold Mucosal Wave and Vibration
Authors: Chung Hye Rhyn , Reddy Neha K. , Manzoor Daniel , Schlegel Patrick , Zhang Zhaoyan , Chhetri Dinesh K. 
Published: July 2023

ObjectivesDespite gross anatomic and histologic differences between human and canine vocal folds, similar wave patterns have been described yet not fully characterized. We reconstructed vocal fold (VF) vibration in a canine hemilarynx and performed histologic examination of the same vocal fold. We demonstrate comparable wave patterns while exploring the importance of certain anatomic architectures.MethodsAn in vivo canine hemilarynx was phonated against a glass prism at low and high muscle activation conditions. Vibration was captured using high‐speed video, and trajectories of VF medial surface tattooed landmarks were 3D‐reconstructed. The method of empirical eigenfunctions was used to capture the essential dynamics of vibratory movement. Histologic examination of the hemilarynx was performed.ResultsOscillation patterns were highly similar between the in vivo canine and previous reports of ex vivo human models. The two most dominant eigenfunctions comprised over 90% of total variance of movement, representing opening/closing and convergent/divergent movement patterns, respectively. We demonstrate a vertical phase difference during the glottal cycle. The time delay between the inferior and superior VF was greater during opening than closing for both activation conditions. Histological examination of canine VF showed not only a thicker lamina propria layer superiorly but also a distinct pattern of thyroarytenoid muscle fibers and fascicles as described in human studies.ConclusionsHistologic and vibratory examination of the canine vocal fold demonstrated human vocal fold vibratory patterns despite certain microstructural differences. This study suggests that the multilayered lamina propria may not be fundamental to vibratory patterns necessary for human‐like voice production.Level of EvidenceNA (Basic science study) Laryngoscope, 134:264–271, 2024
Late laryngeal dysfunction in head and neck cancer survivors
Authors: Almas Sarah , Jeffery Caroline C. 
Published: July 2023

AbstractObjectivesHead and neck cancer (HNC) survivorship issues are areas of increasing research interest. Laryngeal dysfunction in HNC patients is particularly important given the importance of the larynx in voice, swallowing, and airway protection. The objective of our study is to characterize late laryngeal dysfunction in a cohort of long‐term HNC survivors.MethodsHNC survivors who were at least 2 years post‐treatment were recruited prospectively for standard collection of videolaryngoscopy findings, videofluoroscopic swallowing studies, and assessment of clinical outcomes. Descriptive statistics were performed and clinical presentation and outcomes were compared between survivors >10 years and <10 years post‐treatment. Additional factor analysis to correlate clinical outcomes with clinical variables was performed.ResultsThirty participants were analyzed with a mean age of 66 years. The majority were male (80%) patients treated for oropharyngeal squamous cell carcinoma (67%). Within the cohort, 43% underwent primary chemoradiation therapy and had 13% radiation alone. Common presenting symptoms included swallowing dysfunction (83%), voice change (67%), and chronic cough (17%). Laryngeal findings on video laryngoscopy include vocal fold motion abnormalities (VFMA) in over half of participants (61%) and mucosal changes in 20%. A weak correlation was found between time since treatment and laryngeal dysfunction (r = .182, p = .34), and no correlation was found between age, sex, time since treatment, or primary site and the presence or absence of VFMA, G‐tube status, or tracheostomy‐tube status.ConclusionLate laryngeal dysfunction in HNC survivors contributes to significant morbidity, is difficult to treat, and remains static decades after treatment for their original cancer.Lay SummaryThe voice‐box, or the larynx, plays an important role in voice, swallowing and airway protection. It is particularly vulnerable to radiation‐related damage and changes. This study demonstrates the sequelae of long‐term damage of the larynx in head and cancer survivors.Level of EvidenceIV.
“WHO LIVES, WHO DIES, WHO TELLS YOUR STORY…”
Authors: Fullhart Randal D. 
Published: July 2023

AbstractThe author is a now‐retired, longstanding leader in the United States Air Force. He relates the leadership of Frances Hesselbein to the lyrics and title of music in the Broadway hit show, Hamilton, about Alexander Hamilton, one of the Founding Fathers of the United States. The author writes: “What might be impossible to document is the ripple outward of her impact and example to the hundreds of thousands…no, millions of people she impacted through simple words, consistently spoken and lived, over the century‐plus of her life.”
REMEMBERING FRANCES HESSELBEIN: A GREAT AMERICAN SPIRIT
Authors: Hale Patric 
Published: July 2023

AbstractThe author, CEO of Consultex Ltd., a socio‐economic consulting and research firm, and a long‐standing publishing executive, recounts his friendship with Frances Hesselbein, which began when Frances was 98 years old. He notes that he learned right away “one of Frances’ secrets to a long, happy, and engaging life—your age has nothing to do with it; what matters is the enthusiasm and joy you find and put into every day you wake up! She didn’t tell me this—she showed me this—and everyone else who ever was fortunate enough to enter into her realm of being!” He writes that “the more I got to know her and the more we spoke together she epitomized a beautiful spirit that embodied all that was good about America.”
SELFLESS SERVICE AND THE CHOICE OF INCLUSION
Authors: Ryan Diane M. 
Published: July 2023

AbstractThe author, the Associate Dean for Programs and Administration in the Jonathan M. Tisch College of Civic Life at Tufts University, first met Frances Hesselbein as Academy Professor, Director of the Eisenhower Leader Development Program, and Deputy Department Head in the Department of Behavioral Sciences and Leadership at the United States Military Academy, West Point: “Frances arrived at West Point on a bright autumn day to speak with cadets and local Girl Scout troops on the meaning of service and I was the lucky one assigned to be her personal escort… I’d heard about truly transformational leaders who have a special quality of making every individual they encounter feel like the most important person in the room. This is exactly how Frances treated everyone she met regardless of title or status. I felt like a 4‐star general that day and many times since.”
DEFINING LEADERSHIP: FRANCES HESSELBEIN’S GIFT TO US
Authors: Clair Guy St. 
Published: July 2023

AbstractThe author, a writer and editor and pioneer in the field known as knowledge services, writes about his friendship with Frances Hesselbein. “She liked,” the author writes, “that my career was in knowledge services – as a consultant, author, and teacher – and she liked connecting that with her work in leadership.” The author is a longtime educator, and “Frances had strong expectations about how young people move into their roles as our future leaders. As a result, she had a passionate interest in working with young people simply because it was how she thought.” She suggested, in 2016, that she and the author would do a conference, “Leadership Imperatives for Millennials,” about leadership and knowledge for the students, held at Frances’ New York office. She was the keynote speaker, and the students’ “reactions were nothing short of phenomenal (in fact, the word ‘mesmerizing’ came up many times as they described the conference).”
First‐time application of droplet digital PCR for methylation testing of the 11p15.5 imprinting regions
Authors: Schlaich Elia , Hubens Wouter H. G. , Eggermann Thomas 
Published: July 2023

AbstractBackgroundBeckwith‐Wiedemann syndrome and Silver‐Russel syndrome are two imprinting disorders caused by opposite molecular alterations in 11p15.5. With the current diagnostic tests, their molecular diagnosis is challenging due to molecular heterogeneity and mosaic occurrence of the most frequent alterations. As the determination of precise (epi)genotype of patients is relevant as the basis for a personalized treatment, different approaches are needed to increase the sensitivity of diagnostic testing of imprinting disorders.MethodsWe established methylation‐specific droplet digital PCR approaches (MS‐ddPCR) for the two imprinting centers in 11p15.5, and analyzed patients with paternal uniparental disomy of chromosome 11p15.5 (upd(11)pat) and other imprinting defects in the region. The results were compared to those from MS‐MLPA (multiplex ligation‐dependent probe amplification) and MS‐pyrosequencing.ResultsMS‐ddPCR confirmed the molecular alterations in all patients and the results matched well with MS‐MLPA. The results of MS‐pyrosequencing varied between different runs, whereas MS‐ddPCR results were reproducible.ConclusionWe show for the first time that MS‐ddPCR is a reliable and easy applicable method for determination of MS‐associated changes in imprinting disorders. It is therefore an additional tool for multimethod diagnostics of imprinting disorders suitable to improve the diagnostic yield.
Effectiveness of Platelet‐Rich Plasma for COVID‐19‐Related Olfactory Dysfunction: A Controlled Study
Authors: Lechien Jerome R. , Saussez Sven , Vaira Luigi A. , De Riu Giacomo , Boscolo‐Rizzo Paolo , Tirelli Giancarlo , Michel Justin , Radulesco Thomas 
Published: July 2023

AbstractObjectiveTo investigate the effectiveness of platelet‐rich plasma (PRP) injection into the olfactory clefts of coronavirus disease 2019 (COVID‐19) patients with persistent olfactory dysfunction (OD).Study DesignControlled study.SettingMulticenter study.MethodsFrom March 2022 to November 2022, COVID‐19 patients with persistent OD were recruited from three European hospitals to undergo PRP injections into the olfactory clefts. Olfactory function was evaluated at baseline and 10 weeks postinjection with the Olfactory Disorder Questionnaire (ODQ) and threshold, discrimination, and identification (TDI) test. Data were compared with a control group of untreated patients.ResultsEighty‐one patients who underwent PRP injection and 78 controls were included. Sixty‐five PRP patients (80.3%) experienced subjective smell improvement after a mean duration of 3.4 ± 1.9 weeks. The parosmia, life quality statement, and ODQ sub‐ and total scores significantly decreased from pre‐ to 10‐week postinjection in the PRP group. The TDI sub‐ and total scores significantly increased 10 weeks postinjection. In controls, the ODQ score did not change over time, while the discrimination, identification, and total TDI scores significantly increase after 10 weeks of follow‐up. The 10‐week TDI and ODQ scores were significantly better in the PRP group compared with the controls.ConclusionPatients who underwent PRP injection reported better 10‐week subjective and objective smell outcomes than controls. Future randomized‐controlled studies using saline injection into the olfactory cleft of controls are needed to determine the superiority of PRP over placebo.
Heparin‐guided binding of vascular endothelial growth factor to supramolecular biomaterial surfaces
Authors: Schmitz Moniek G. J. , Ibrahim Dina M. , Bartels Paul A. A. , Twisk Simone A. E. , Smits Anthal I. P. M. , Bouten Carlijn V. C. , Dankers Patricia Y. W. 
Published: July 2023

AbstractGrowth factors can steer the biological response to a biomaterial post implantation. Heparin is a growth factor binding molecule that can coordinate growth factor presentation to cells and therefore is able to regulate many biological processes. One way to functionalize biomaterials with heparin and growth factors is via a supramolecular approach. Here, we show a proof‐of‐concept study in which a supramolecular approach based on ureido‐pyrimidinone (UPy) was used, which allows for modular functionalization. PCLdiUPy was functionalized with a UPy‐modified heparin binding peptide (UPy‐HBP) to facilitates binding of heparin, which in turn can bind vascular endothelial growth factor (VEGF) via its heparin binding domain. The adsorption of both heparin and VEGF were studied in two different functionalization approaches (pre‐complex and two‐step) and at different molecular ratios. Quartz crystal microbalance with dissipation energy adsorption data showed that VEGF and pre‐complexed heparin:VEGF adsorbed non‐specifically, with no distinguish between non‐specific adsorption and heparin guided‐adsorption. On the biological side, heparin guided‐adsorption of Heparin:VEGF enhanced HUVECs surface coverage as compared to non‐specific adsorption. These results provide a detailed insight on the molecular sandwich which is useful for new design strategies of supramolecular biomaterials with well‐controlled immobilization of different growth factors.