bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of recurrent severe thrombosis caused by the duplication of the Factor IX gene
Authors: Wei Xuqian , Zhang Houliang , Chen Weibin , Zhang Jian , Dai Jing 
Published: August 2023

Abstract
Here we studied a patient who experienced three different episodes of severe thromboses, some of which were life-threatening (pulmonary artery thrombosis, portal and mesenteric vein thrombosis, and arterial thrombosis of the lower leg). Blood levels of lupus anticoagulants, anticardiolipin antibodies, Protein C: C, Protein S: C, AT: C, FVIII: C, FIX: C, and homocysteine were also assessed. We screened 35 genes linked to thrombosis using a mix of first- and second-generation sequencing. Mutations were subsequently confirmed and localized using an Affymetrix CytoScan 750 microchip to identify gene imbalances. We discovered a duplication of the FIX gene on the X chromosome of the patient through genetic testing. The mutation was a 756 kb duplication that spanned the FIX gene in region q27.1 of the X chromosome and causes a gene imbalance. The precise coordinates of the duplication are arrGRCh37Xq27.1(138548177 139304097) *2. The repeat includes the full FIX gene, thus, this suggested the patient had two functional copies of FIX. An identical repetition was found in the patient's mother's genetic test, indicating that this mutation was inherited. Both the patient and his mother had high, but variable, plasma FIX:C levels that promote coagulation. The patient's frequent, severe thromboses may be brought on by the duplication of a big portion of the FIX gene. The first worldwide report of this mutation.
In-person and virtual adaptation of an interprofessional palliative care communications skills training course for pediatric oncology clinicians
Authors: Moody Karen M. , Andersen Clark , Bradley Julie , Draper Lauren , Garrington Timothy , Gill Jonathan , Harrison Douglas , Hayashi Masanori , Heaton Amy , Holladay Cynthia , Lion Alex , Rajan Alakh , Rozo Beatriz , Runco Daniel , Salvador Laura , Ferguson Verna , Arnold Robert 
Published: August 2023

Abstract
Purpose
Effective, empathic communication is crucial for pediatric oncology clinicians when discussing palliative and end-of-life (PC/EOL) care with parents of children with cancer. Unfortunately, many parents report inadequate communication at these distressing times. This study evaluates the communication skills training (CST) clinicians received to deliver a PC/EOL communication intervention as part of a multi-site randomized-controlled trial (RCT).
Methods
Clinicians from eight sites formed dyads (one physician and one nurse [RN] or advanced practice provider [APP]) and were trained over 3 days (in-person or virtually). Training was adapted from VitalTalkTM and included didactic instruction, videos, visual aids, and dedicated time to practice with simulated patients. Study participants completed a confidential, post-training online evaluation survey. A self-reported quality assurance checklist was used to measure fidelity to the communication protocol when delivered to parents during the RCT.
Results
Thirty clinicians completed training; 26 completed post-training surveys including twelve (46.1%) physicians, 8 (30.8%) RNs and 6 (23.1%) APPs. Most were female (65.4%); white (80.8%), not Latinx (88.5%); 40-50 years old (53.9%); and in practice over 10 years (65.4%). Nine (34.6%) trained in-person; the rest trained virtually. Ninety-two percent reported the course was valuable or very valuable for developing their PC/EOL communication skills and 96% reported learning something new. Dyads trained virtually had similar fidelity to those trained in-person (95% and 90% respectively) when delivering the PC/EOL communication intervention to parents.
Conclusion
This PC/EOL CST was valuable for improving pediatric oncology clinicians’ communication skills, successfully implemented in-person and virtually, and translated effectively into practice.
Cultural Adaptation of an Integrated Eating Disorders Prevention and Healthy Weight Management Program
Authors: Simpson Courtney C. , Boutté Rachel L , Burnette C. Blair , Weinstock Madison , Mazzeo Suzanne , Goel Neha 
Published: August 2023

Abstract
Background: Both eating disorder (ED) prevention and weight management interventions often focus on the thin ideal. Yet, many Black and Latinx women do not view thinness as their body ideal. This study used focus groups to investigate the influence of race, ethnicity, and culture on appearance ideals and inform the cultural adaptation and integration of two established programs addressing EDs and weight management: the Body Project, and Healthy Weight Interventions.
Methods:White (n=10), Black (n=14), and Latinx (n=6) women participated in racially and ethnically homogenous focus groups.
Results: Thematic analysis identified several themes, including: (1) diverse beauty standards across groups, (2) lack of acknowledgement of racialized beauty standards in prevention and cultural appropriation, (3) culturally-specific impacts of standards, (4) harm of appearance-related comments, (5) limitations of available resources, (6) stigma/minimization of mental health, (7) barriers to inclusive programming, and (8) facilitators of inclusive programming.
Conclusions: Results suggested that current programs’ emphasis on thinness, limit their relevance for women of color, and perpetuate the misconception that EDs primarily affect White women. Findings highlight the need for culturally responsive prevention.
Investigation on the effect of Xylopia aethiopica ethanol seed extract on Aluminum chloride induced uterus and gonadotropins toxicity in adult female Wistar rats
Authors: Leko Bankole Japhet , Idaguko Chika Anna , Aroge Iyanuoluwa Precious 
Published: August 2023

Abstract

Introduction:
Reproductive disorders in females as a result of exposure to heavy metals are considered an important public health and social problem.
Aim
The study was to determine the level of influence of Xylopia aethiopica extracts on the female reproductive hormone, and histological changes in the uterus following administration of Aluminum chloride (AICI3).
Materials and methods
Twenty female Wistar rats were randomly divided into 4 groups of five rats. Group 1 served as normal control, groups 2, 3, 4 were administered 150mg/kg Aluminum chloride (AICI3). Group 2 served as positive control, while groups 3 and 4 were administered 50 mg/kg and 100 mg/kg extract of Xylopia aethiopica respectively. Administration of extract was done through oral route for 21 days, after which the animals were sacrificed. Hormonal assay such as Luteinizing hormone, Progesterone, Follicle Stimulating Hormone, Estradiol and histology of the uterus were evaluated.
Results
This revealed (AICI3) decrease the levels of FSH, LH, Estradiol and Progesterone. These results was further supported by histological observation that showed the thickenings and degeneration in the endometrial epithelial in the uterus caused by Aluminum chloride. Xylopia aethiopica treated groups showed a significant (p < 0.05) increase in FSH, LH and Progesterone levels, while the Estradiol level was increase but it was not significant. Histologically, Xylopia aethiopica reduced thickenings and degeneration in the endometrial epithelial of the uterus caused by Aluminum chloride.
Conclusion
The finding demonstrated that treatment using Xylopia aethiopica may minimizes the toxic effect of Aluminum chloride on the female reproductive hormones and the uterus.
Wearable cellulose textile matrix self-powered biosensor sensing lactate in human sweat
Authors: 夏 尊斌 , Zuo Wei , Li Haiying , Qiu Liewei , Mu Ruihua , Wang Qian , Liu Hongchen , Wang Huixin , Hui yuchen 
Published: August 2023

Abstract
A lactate self-powered biosensor was assembled for the detection of lactate in sweat based on flexible textile matrix enzymatic electrodes. A porous three-dimensional electrode with high flexibility and electrical conductivity was obtained by decorating composites of reduced graphene oxide and carboxylate multi-walled carbon nanotubes onto a cellulose fabric substrate. The excellent enzyme embedding method using gel electrolyte showed a Michaelis-Menten constant of 1.46 mM, which implies that lactate dehydrogenase had a high enzymatic activity. The assembled self-powered lactate biosensor showed a sensitivity of 3.16 µW mM− 1 cm− 2 in the 0–10 mM linear range and a detection limit of 9.49 µM (S/N = 3). Meanwhile, the biosensor has good tensile flexural stability, selectivity, and long-term stability. The lactate self-powered biosensor recovered 99.66–108.43% of lactate from real sweat with a relative standard deviation of less than 4.65% when integrated into clothing. The biosensor is promising for sensing lactate in real sweat.
Engineering Saccharomyces boulardii for cell surface display of heterologous protein
Authors: Shin Jamin , Jin Yong-Su , Kim Soo Rin 
Published: August 2023

Abstract

Purpose:
Saccharomyces boulardii shares >99% similar genome sequences with S. cerevisiae, although there are some different genetic and metabolic elements between the two strains. Much is known about the cell surface display system of S. cerevisiae, but S. boulardii is still lacking. Therefore, in this study, cell surface display of S. boulardii is compared with different anchor proteins.
Methods:
For visualization of the cell surface display system, six strains expressing the enhanced green fluorescent protein (Egfp) and an anchor protein as a fusion protein were constructed. Then a heterologous protein, endo-inulinase, was expressed with selected anchor proteins through fluorescence intensity comparison.
Results:
Analysis by fluorescence microscopy revealed that the anchor proteins Cwp2 and Sed1 exhibited the highest fluorescence intensity. Furthermore, expressed selected anchor proteins and heterologous protein, endo-inulinase, the two mutant strains could degrade and consume almost inulin in 48 h.
Conclusion:
Through the expression of endo-inulinase, we confirmed that not only Egfp but also heterologous protein is well expressed, and successfully built a S. boulardii cell surface display system.
Circumstances of Occurrence and Factors Associated with Occupational Exposure to Body Fluids in District Hospitals (Yaoundé, Cameroon)
Authors: Cheuyem Fabrice Zobel Lekeumo , Ndungo Jonathan Hangi , Lyonga Emilia Enjema , MBOPI–KEOU François–Xavier , Takougang Innocent 
Published: August 2023

AbstractBackground:Though of worldwide occurrence, accidental exposure to body fluids affects developing countries most, especially sub-Saharan Africa. Exposure to body fluids in occupational settings affects the safety and wellbeing of healthcare workers, hindering the quality of healthcare. The aim of the present investigation was to identify the tasks prone to occupational exposure to body fluids and associated risk factors.Methods:A cross sectional descriptive study was carried out from January to April 2022 in six District Hospitals in Yaoundé. A self-administered questionnaire was addressed to consenting healthcare workers. Data related to exposure to blood and body fluid were analysed using loglinear model logistic regression on R statistic version 4.2.3. Statistical significance was considered forp-value < 5%.Results:A total of 217 participants were enrolled in this study. The main circumstances of percutaneous injury were handling needles while providing care (30.9%) and surgical procedures (19.5%). Fatigue related to long working hours (32.9%), stress (22.8%) and lack of personal protective equipment (17.4%) were reported determinants of percutaneous injuries. Poor compliance with standard precautions, such as washing scalpel for reuse (OR = 7.56) were associated with needles stick injuries. Working in the surgical department was associated with exposure to splash (AOR = 4.72) and percutaneous injury (OR=3.11).Conclusion:Exposure to body fluids involved splashes, needlestick and sharp injuries, occurring mainly in the surgical departments. There is an urgent need to design and implement preventive activities to reduce the risk of infections related to occupational exposure to body fluids among healthcare workers.
Charge transfer complex mediated conversion of CO2 to CO fuel
Authors: Cao Xuebo , Wang Kai , Chu Mingming , Wu Yuanyuan , Zhang Xingtian , Yan Zheng 
Published: August 2023

Abstract
Carbon monoxide (CO) is a versatile feedstock for the production of synthetic fuels using Fischer-Tropsch (FT) chemistry and a broader range of compounds using acylation chemistry. Commercial CO derives from natural gas, petroleum, and coal. In order to mitigate the dependence on fossil resources and assure a sustainable future, scientific communities are indefatigably pursuing electrochemical/photochemical conversion of ubiquitous carbon dioxide (CO2) back to useful chemicals like CO. But large-scale deployment of the technologies is hindered by low production rate, low conversion efficiency, and rigorous demands on the catalysts/devices. Here we show a chemical reduction strategy capable of rapid and large-scale conversion of CO2 into combustible CO (chromatographic purity > 99%) at ambient temperature. The process only involves three industrial materials including copper (Cu), zinc (Zn), and N-methylimidazole (NMI) and exempts special installation, thus favoring practical implementation. The unprecedented efficiency is attributed to the mediation of the charge transfer complex [CuI∙∙∙NMI•‒] formed through spontaneous charge transfer interaction between NMI and Cu. [CuI∙∙∙NMI•‒] is an efficient mediator that can transform inert CO2 to CO2−CO2•‒ and stabilize this key intermediate. Zn then induces the reductive disproportionation of CO2−CO2•‒ into CO.
Avoiding stoma creation due to super-morbid obesity: a report of two surgical cases of colorectal cancer
Authors: Fujimoto Naoto , Ogino Takayuki , Sekido Yuki , Takeda Mitsunobu , Hata Tsuyoshi , Hamabe Atsushi , Miyoshi Norikatsu , Uemura Mamoru , Mizushima Tsunekazu , Doki Yuichiro , Eguchi Hidetoshi 
Published: August 2023

Abstract
Background: A stoma is commonly created in patients with a high risk of anastomotic leakage. However, patients with obesity have a higher incidence of stoma-related complications, and the decision to create a stoma should be carefully considered. We report two cases of patients with colorectal cancer and super-morbid obesity wherein stoma creation was avoided.
Case presentation: Case 1 involved a 52-year-old male patient with a body mass index (BMI) of 41.8 kg/m2 who underwent a robotic-assisted laparoscopic low anterior resection after neoadjuvant chemotherapy for lower rectal cancer. Although temporary diverting ileostomy was initially considered, stoma creation was skipped intraoperatively, considering the complication risk-benefit ratio. Case 2 involved a 42-year-old female patient with a BMI of 64 kg/m2 who underwent open partial colon resection for descending colon cancer complicated by colonic perforation and abscess formation. The patient was considered to be at high risk of stoma-related complications due to high mobility of the abdominal wall; therefore, we decided not to create a stoma preoperatively.
Conclusion: Considering the high risk of stoma-associated complications, avoiding stoma creation and implementing preventive measures against potential complications are alternative options for patients with super-morbid obesity.
Cone-beam computed tomography of accessory canals of the canalis sinuosus and analysis of the related risk factors
Authors: sun Zhenwei , Li Dan , Zhang Xuan , Zhang Jiaxin , He Chunyan 
Published: August 2023

Abstract
Purpose
Cone-beam computed tomography (CBCT) was used in this study for evaluating the diameter, prevalence, spatial location, and risk factors of the accessory canal (AC) of the canalis sinuosus.
Methods
A comprehensive assessment of the incidence rate, diameter, three-dimensional (3D) spatial location, and direction of travel of AC was performed on 1003 CBCT images. The CBCT data were used to reconstruct a 3D model of the maxilla to determine the alveolar bone volume. The obtained data were further analyzed and processed.
Results
AC was present in 50.1% of images. Male patients more frequently had ACs than female patients did (P < 0.01) and was positively correlated with the maxillary alveolar bone volume (P < 0.001, OR 1.554). Age or nasopalatine canal diameter were not significantly associated with the occurrence of AC (P > 0.05). Among the 502 patients with AC, AC was present on the left side, right side, and bilaterally in 189, 98, and 215, respectively. The maximum number of ACs observed per individual was eight. The average AC diameter was 0.89 ± 0.26 mm (minimum, 0.5 mm; maximum, 2.02 mm).
Conclusions
As the prevalence of AC and its trajectory display considerable variation among individuals, surgeons must consider the possibility of the presence of AC when devising surgical plans involving the anterior maxillary region.
Sunset Yellow protects against oxidative damage in human keratinocyte-derived HaCaT cells and exhibits chemopreventive efficacy in DMBA/TPA-induced skin carcinogenesis
Authors: Gupta Shailendra , Singh Saurabh , Yadav Sarika , Cavallo Celine , Mourya Durgesh , Shukla Pallavi , Chaudhary Romil , Maurya Gyan , Müller Ronja , Rohde Lilly , Mishra Aradhana , Tripathi Anurag 
Published: August 2023

Abstract
Background: Skin cancer and other pathophysiological conditions of the skin are caused by inflammation. Sunset Yellow (SY) exhibits immunomodulatory characteristics, evidenced by its capacity to partially inhibit the secretion of proinflammatory cytokines, regulate immune cell populations, and modulate the activation of lymphocytes. The aim of our study was to investigate the potential anti-inflammatory properties of SY through in-silico, in vitro & physiochemical test systems, and to investigate further its implications in DMBA/TPA-induced two-step skin carcinogenesis model.
Result: In vitro experiments showed that pre-treatment of SY significantly enhanced the cell viability of HaCaT cells when exposed to tertiary-Butyl Hydrogen Peroxide (tBHP). The increase in cell viability was accompanied by decreased levels of ROS, restoration of deregulated mitochondrial membrane potential, and significantly reduced DNA damage in (SY+ tBHP) treated cells. Further mechanistic investigation revealed that SY exhibited antioxidant properties. Through potentiometric titrations, the standard reduction potential (Eº) of SY was observed to be 0.211V. Additionally, this result was also confirmed using DPPH chemical antioxidant activity test. Next, we tested the implications of these findings in DMBA/TPA-induced two-step skin carcinogenesis animal model. The mice received topical application of SY (0.025%, 0.05%, and 0.1%) in conjunction with DMBA/TPA treatment for 21 weeks. The tumor incidence and body weight were evaluated at regular time intervals. SY remarkably reduced the tumor average latency period, tumor incidence, tumor yield and tumor burden in a dose-dependent manner. Finally, several in silico targets of SY were also identified, which could shed some light on the molecular mechanism triggered by SY as an antioxidant and chemo-preventive agent.
Conclusions: Conclusively, we repurposed SY for the antioxidant and anti-genotoxic properties along with its chemoprotective effect on skin cancer.
Three-Dimensional Wideband Non-Stationary Channel Modeling for Unmanned Aerial Vehicle Communication under Flight-Induced Jitter Effects
Authors: rui liu , Jie Zhou , Lei Xu , Zilong Zhou , Gen-hu Shao 
Published: August 2023

Abstract
In view of the significant impact of flight-induced jitter on the performance of unmanned aerial vehicle (UAV) communication systems, we propose a three-dimensional wideband non-stationary channel model. The model takes into account the line-of-sight (LoS) and non-line-of-sight (NLoS) components in the transmission link from the UAV transmitter to the UAV receiver. Both the transmitter and the receiver are equipped with uniform array antennas. In contrast to previous studies that focus solely on UAV pitch jitter models, the UAV's jitter attitude is simulated as random fluctuations in pitch, azimuth, and roll angles. The rotational jitter is modeled using a sinusoidal random process. Furthermore, derived the typical statistical characteristics of the proposed model, including spatial cross-correlation function(CCFs) and temporal autocorrelation function(ACFs). The numerical simulation results are then compared with those of existing relevant models, demonstrating the effectiveness of the proposed channel model.
Successful chimney endovascular aortic repair with reconstruction of three visceral branches for juxtarenal abdominal aortic pseudoaneurysm after trans-thoracoabdominal esophagectomy
Authors: Goto Takasumi , Fujimura Hironobu , Shintani Takashi , Shibuya Takashi 
Published: August 2023

Abstract

Background
Conventional graft replacement for a juxtarenal abdominal aortic aneurysm remains challenging for high-risk patients since it often requires the reconstruction of some visceral arteries.
Case Presentation
A 76-year-old woman was diagnosed with an 87 x 48 mm juxtarenal abdominal aortic pseudoaneurysm. Open graft replacement was contraindicated because of frailty and a past history of trans-thoracoabdominal esophagectomy. Chimney endovascular aortic repair (CEVAR) with three chimney endografts was successfully performed without any endoleaks and each visceral circulation was kept intact. The patient was discharged uneventfully on postoperative day 8.
Conclusions
CEVAR can be a useful surgical option instead of conventional operations, especially for high-risk cases
Xanthurenic acid as a preclinical diagnostic marker and drug target of Alzheimer's disease in rats and mice and associated effects of Schizandrin
Authors: Zhao Qiqi , Ma Zhiming , Lu Shengwen 
Published: August 2023

Abstract
Alzheimer's disease (AD), a multipathogenic and irreversible neurodegenerative disease, threatens human health because a well-developed and compelling ‘theragnostic’ marker for monitoring biochemical events in this disease is lacking. In this experiment, high excretion of xanthurenic acid was found quantitatively before neurodegeneration in two classic AD models, including APP/PS1 transgenic mice and a chemical-induced rat model, based on metabolomic analysis coupled with UPLC-TQD-MS/MS. The discovery was beyond the recorded pathological process, including Aβ42 deposition, astrocyte inflammatory hyperplasia and neuron loss, and it can be used not only as a diagnostic marker for the preclinical stage of AD but also as a target reflecting the disease-modifying effect of lead compounds for preventing AD. In this way, Schisandrin, a neuroprotective agent from Schisandra chinensis (Turcz.) Baill, was proven to adjust the level of xanthurenic acid in urine significantly in the present study. The predictability, treatment relevance, stability, reproducibility and accessibility of urine xanthurenic acid in our experiment indicates its feasibility as a potential diagnostic marker and drug target for the preclinical stage of AD.