bluuebunny/crossref_metadata_2025_split huggingface dataset:
Humoral responses after primary and booster SARS‐CoV‐2 inactivated vaccination in patients with chronic hepatitis B virus infection: A longitudinal observational study
Authors: Chen Zhiwei , Huang Tianquan , He Taiyu , Zha Guanhua , Zhu Qian , Zhang Gaoli , Xiang Dejuan , Chen Min , Li Hu , Ling Ning , Lan Yinghua , Shi Xiaofeng , Zhang Dazhi , Xu Pan , Pan Qingbo , Song Rui , Cao Junxiong , Zhang Yingzhi , Xiang Hongyan , Feng Yali , Yang Ziqiao , Zhang Biqiong , Shen Wei , Cai Dachuan , Peng Mingli , Hu Peng , Ren Hong 
Published: March 2023

AbstractGiven the pandemic of severe acute respiratory syndrome coronavirus 2 Omicron variants, booster vaccination (BV) using inactivated virus vaccines (the third dose) has been implemented in China. However, the immune responses after BV, especially those against Omicron, in patients with chronic hepatitis B virus (HBV) infection (CHB) are unclear. In this prospective longitudinal study, 114 patients with CHB and 68 healthy controls (HCs) were recruited after receiving inactivated vaccination. The anti‐receptor‐binding domain (RBD) immunoglobulin G (IgG), neutralizing antibodies (NAbs), neutralization against Omicron (BA2.12.1, BA.4/5), and specific B/T cells were evaluated. In patients, anti‐RBD IgG was elevated significantly after BV; the titers were as high as those in HCs. Similar results were obtained for the NAbs. However, compared with that against wild type (WT), the neutralization against Omicron was compromised after BV. The frequency of RBD+ atypical memory B cells increased, but spike‐specific cluster of differentiation 4+/8+ T cells remained unchanged after BV. Moreover, no serious adverse events or HBV reactivation were observed after BV. These results suggest that BV significantly enhanced antibody responses against WT; however, it resulted in compromised antibody responses against Omicron in patients with CHB. Hence, new all‐in‐one vaccines and optimal vaccination strategies should be studied promptly.
Performance analysis of short channel effects immune JLFET with enhanced drive current
Authors: Raj Abhishek , Singh Kunal , Sharma Shashi Kant 
Published: March 2023

AbstractThis manuscript reports analog/RF and noise analysis of a novel junctionless field effect transistor. To reduce the gate induced drain leakage (GIDL), step‐gate‐oxide structure is used with a high‐k buried oxide (BOX) which subsequently reduces IOFF. The off current and gate capacitance is reduced by including step‐gate‐oxide in high k‐BOX JLFET due to increase in the band to band tunneling (BTBT) width. The DIBL is reduced significantly to 0.014 which is 60% less as compared to high‐k BOX JLFET (HB JLFET). For different length and thickness of the step‐gate‐oxide, the analog/RF and noise performance of the step‐gate‐oxide JLFET with high‐k BOX (SGO HB JLFET) have also been investigated in detail. Silvaco TCAD simulation of the proposed structure for 20 nm channel length shows an extremely high ION/IOFF ratio of ~108. The subthreshold swing for the simulated structure was also found to be considerably low (82 mV/dec).
Effect of transactional and transformational leadership on talent engagement: Mediating role of talent development
Authors: Ali Mohammad , Ullah Muhammad Shariat , Haque Amlan 
Published: March 2023

AbstractLeadership is an essential antecedent to talent development and engagement, but there is a dearth of literature related to transactional or transformational leadership and talent management (TM). The paper investigates the impact of transactional and transformational leadership on talent engagement and the mediating role of talent development between them. Based on a cross‐sectional survey of 450 employees, the study employs structural equation modeling using social exchange theory as a lens to test the hypothesized relationship among transactional leadership, transformational leadership, talent development and engagement. The results confirmed that transactional leadership is positively related to talent engagement and development while transformational leadership is neither associated with talent engagement nor talent development. Moreover, talent development mediates the relationship between transactional leadership and talent engagement. The study outcomes are helpful to managers and leaders in comprehending the significance of transactional leadership in developing and engaging talents.
Fractional CO2‐laser versus microneedle radiofrequency for acne scars: A randomized, single treatment, split‐face trial
Authors: Hendel Kristoffer , Karmisholt Katrine , Hedelund Lene , Haedersdal Merete 
Published: March 2023

AbstractBackgroundAblative fractional CO2 laser (AFL) is an established first‐line energy‐based treatment for acne scars. Microneedle radiofrequency (MNRF) is an emerging treatment, also targeting the skin in fractions. No studies have so far compared AFL with MNRF for acne scars in a direct controlled, side‐by‐side comparison. In this study, we compared AFL and MNRF treatments for acne scars in a randomized split‐face trial with blinded response evaluation, objective measures, and patient‐reported outcomes.Study Design/Materials and MethodFifteen patients with moderate to severe acne scars were included. At baseline each patient had two similar test areas identified, these were randomized to receive a single treatment with either AFL or MNRF. Standardized multilayer techniques were applied with AFL and MNRF, first targeting the scar base, thereafter the entire scar area. Outcome measures included blinded evaluation of clinical improvement of scar texture (0–10 scale) at 1‐ and 3‐months follow‐up, local skin reactions (LSR), pain according to Visual Analogue Scale (VAS), skin integrity quantified by transepidermal water loss, and patient satisfaction.ResultsFifteen patients completed the study with a median test area size of 24.6 cm2 (interquartile range [IQR] 14.9–40.6). A single treatment with AFL or MNRF equally resulted in a median 1‐point texture improvement after 3 months follow‐up (p < 0.001). Best responders achieved up to a 3‐point improvement (n = 3 test areas, 10% of treatment areas). Erythema and loss of skin integrity was more intense after AFL compared with MNRF after 2–4 days (p < 0.001). Patients reported MNRF (VAS 7.0) to be significantly more painful than AFL (5.5) (p = 0.009). Patients were generally satisfied with the overall outcome on a 10‐point scale at median 6 for both treatments (IQR 5–7).ConclusionAFL and MNRF treatments are equally effective at improving texture in skin with acne scars. AFL resulted in more pronounced LSRs whereas MNRF was more painful. Patients were generally satisfied with the overall outcome.
Study on the Friction and Wear Properties of PEEK against Cortical Bone Tissue Under Different Lubricating Conditions
Authors: Zhang Xiao , Ma Xiaochen , Lin Qianming , Xiao Lin , Wang Shuhan , Yang Han , Liao Liqiong , Liu Yuhong , Zhang Chao , Song Jian 
Published: March 2023

AbstractThe tribological behavior between orthopedic implants and cortical bone is important but usually neglected. Poly(ether‐ether‐ketone) (PEEK) is a promising material for orthopedic applications. To further understand and improve the interfacial tribological properties between PEEK implant and host bone tissue, a PEEK‐cortical bone tribo‐pair is designed and fabricated. The frictional and wear performance of such tribo‐pair is investigated under different lubricants, i.e., simulated body fluid (SBF), calf serum (CS), hyaluronic acid (HA), and mucin (MUC). The results suggest that MUC solution can be utilized as an artificial natural synovial fluid to improve the tribological properties of PEEK‐based implants.
Development of a Supramolecular Hydrogel for Intraperitoneal Injections
Authors: Wintjens Anne G. W. E. , Fransen Peter‐Paul K. H. , Lenaerts Kaatje , Liu Hong , van Almen Geert C. , van Steensel Sebastiaan , Gijbels Marion J. , de Hingh Ignace H. J. T. , Dankers Patricia Y. W. , Bouvy Nicole D. 
Published: March 2023

AbstractLocal intraperitoneal drug administration is considered a challenging drug delivery route. The therapeutic efficiency is low, mainly due to rapid clearance of drugs. To increase the intraperitoneal retention time of specific drugs, a pH‐sensitive supramolecular hydrogel that can act as a drug delivery vehicle is developed. To establish the optimal formulation of the hydrogel and to study its feasibility, safety, and tissue compatibility, in vitro, postmortem, and in vivo experiments are performed. In vitro tests reveal that a hydrogelator formulation with pH ≥ 9 results in a constant viscosity of 0.1 Pa·s. After administration postmortem, the hydrogel covers the parietal and visceral peritoneum with a thin, soft layer. In the subsequent in vivo experiments, 14 healthy rats are subjected to intraperitoneal injection with the hydrogel. Fourteen and 28 days after implantation, the animals are euthanized. Intraperitoneal exposure to the hydrogel is not resulted in significant weight loss or discomfort. Moreover, no macroscopic adverse effects or signs of organ damage are detected. In several intra‐abdominal tissues, vacuolated macrophages are found indicating a physiological degradation of the synthetic hydrogel. This study demonstrates that the supramolecular hydrogel is safe for intraperitoneal application and that the hydrogel shows good tissue compatibility in rats.
Targeted nanopore sequencing enables complete characterisation of structural deletions initially identified using exon‐based short‐read sequencing strategies
Authors: McClinton Benjamin , Crinnion Laura A. , McKibbin Martin , Mukherjee Rajarshi , Poulter James A. , Smith Claire E. L. , Ali Manir , Watson Christopher M. , Inglehearn Chris F. , Toomes Carmel 
Published: March 2023

AbstractBackgroundThe widespread adoption of exome sequencing has greatly increased the rate of genetic diagnosis for inherited conditions. However, the detection and validation of large deletions remains challenging. While numerous bioinformatics approaches have been developed to detect deletions from whole ‐ exome sequencing and targeted panels, further work is typically required to define the physical breakpoints or integration sites. Accurate characterisation requires either expensive follow ‐ up whole ‐ genome sequencing or the time ‐ consuming, laborious process of PCR walking, both of which are challenging when dealing with the repeat sequences which frequently intersect deletion breakpoints. The aim of this study was to develop a cost‐effective, long‐range sequencing method to characterise deletions.MethodsGenomic DNA was amplified with primers spanning the deletion using long‐range PCR and the products purified. Sequencing was performed on MinION flongle flowcells. The resulting fast5 files were basecalled using Guppy, trimmed using Porechop and aligned using Minimap2. Filtering was performed using NanoFilt. Nanopore sequencing results were verified by Sanger sequencing.ResultsFour cases with deletions detected following comparative read‐depth analysis of targeted short‐read sequencing were analysed. Nanopore sequencing defined breakpoints at the molecular level in all cases including homozygous breakpoints in EYS, CNGA1 and CNGB1 and a heterozygous deletion in PRPF31. All breakpoints were verified by Sanger sequencing.ConclusionsIn this study, a quick, accurate and cost ‐ effective method is described to characterise deletions identified from exome, and similar data, using nanopore sequencing.
Identification of a homozygous frameshift mutation in the FGF3 gene in a consanguineous Iranian family: First report of labyrinthine aplasia, microtia, and microdontia syndrome in Iran and literature review
Authors: Jamshidi Fereshteh , Shokouhian Ebrahim , Mohseni Marzieh , Kahrizi Kimia , Najmabadi Hossein , Babanejad Mojgan 
Published: March 2023

AbstractBackgroundTo date, over 400 syndromes with hearing impairment have been identified which altogether constitute almost 30% of hereditary hearing loss (HL) cases around the globe. Manifested as complete or partial labyrinthine aplasia (severe malformations of the inner ear structure), type I microtia (smaller outer ear with shortened auricles), and microdontia (small and widely spaced teeth), labyrinthine aplasia, microtia, and microdontia (LAMM) syndrome (OMIM 610706) is an extremely rare autosomal recessive condition caused by bi‐allelic mutations in the FGF3 gene.MethodsUsing the whole‐exome sequencing (WES) data of the proband, we analyzed a consanguineous Iranian family with three affected members presenting with congenital bilateral HL, type I microtia, and microdontia.ResultsWe discovered the homozygous deletion c.45delC in the first exon of the FGF3 gene, overlapping a 38.72 Mb homozygosity region in chromosome 11. Further investigations using Sanger sequencing revealed that this variant co‐segregated with the phenotype observed in the family.ConclusionHere, we report the first identified case of LAMM syndrome in Iran, and by identifying a frameshift variant in the first exon of the FGF3 gene, our result will help better clarify the phenotype–genotype relation of LAMM syndrome.
Genetic analysis of periventricular nodular heterotopia 7 caused by a novel NEDD4L missense mutation: Case and literature summary
Authors: Liu Juan , Hu Jihong , Duan Yaqing , Qin Rong , Guo Chunguang , Zhou Hongtao , Liu Hua , Liu Chunlei 
Published: March 2023

AbstractBackgroundNeurodevelopmental disorders associated with periventricular nodular heterotopia (PVNH) are characterized by phenotypic and genetic heterogeneity. NEDD4L mutation can lead to PVNH7. However, at present, only eight NEDD4L pathogenic variants have been identified across 15 cases of PVNH7 worldwide. Given this dearth of evidence, the precise correlations between genetic pathogenesis and phenotypes remain to be determined.MethodsThis report discusses the case of a 19‐month‐old male child with cleft palate, seizures, psychomotor retardation, and hypotonia, for whom we verified the genetic etiology using Trio‐whole‐exome and Sanger sequencing to analyze the potential pathogenicity of the mutant protein structure. Mutant plasmids were constructed for in vitro analyses. After transfection into human 293 T cells, the mutant transcription process was analyzed using real‐time PCR (RT‐PCR), and levels of mutant protein expression were examined using western blotting (WB) and immunofluorescence (IF) experiments.ResultsGenetic analyses revealed a novel missense mutation Gln900Arg, located in the homologous to E6‐APC terminal (HECT) domain of NEDD4L and that the parents were wild‐type, suggestive of a de novo mutation. The variant was predicted to be pathogenic by bioinformatics software, which also suggested alterations in the structural stability of the mutant protein. RT‐PCR results indicated that the mutation did not affect mRNA expression, whereas WB and IF results indicated that the level of mutant protein was significantly reduced by 41.07%.ConclusionFunctional experiments demonstrated that Gln900Arg probably did not lead to transcriptional abnormalities in this patient, instead leading to increased ubiquitination activity owing to the constitutive activation of the HECT domain, thereby promoting protein degradation. Extensive clinical reports should be generated for patients presenting with PVNH and/or polymicrogyria, developmental delay, syndactyly, and hypotonia to increase the pool of evidence related to NEDD4L.
The Hypoglycemic Activities and Underlying Mechanisms of Two Saponins‐Rich Components from Fried Ziziphus jujuba Mill. Kernel
Authors: Li Yi‐Meng , Hao Ke‐xin , Xie Hong , Jiang Jian‐Guo 
Published: March 2023

ScopeDried Ziziphus jujuba Mill. kernel is a potential natural source of nutraceutical and therapeutic agents in China. Recent researches have shown that the saponins of dried Z. jujuba Mill. kernel (SZJs: SZJ‐1 and SZJ‐2) have various biological effects. However, the hypoglycemic activities and underlying mechanisms of SZJs remain obscure.Method and resultsIn the current study, two saponins SZJ‐1 and SZJ‐2 mainly composed of betulinic acid, spinosin, jujuboside A, and jujuboside B are extracted and is olated from dried Z. jujuba Mill. kernel. The SZJ‐1 and SZJ‐2 could significantly inhibit the activities of digestion enzymes α‐glucosidase and α‐amylases. The hypoglycemic ability of SZJ‐1 and SZJ‐2 is further investigated and the results show that SZJ‐1 and SZJ‐2 can improve the hyperglycemic by increasing the glucose consumption, improving the superoxide dismutase (SOD), hexokinase (HK), pyruvate kinase (PK) activities, and decrease the MDA content of insulin resistant HepG2 cells. Furthermore, SZJ‐1 and SZJ‐2 can activate the phosphorated adenosine 5′‐monophosphate (AMP)‐activated protein kinase α (p‐AMPK), phosphoinositide 3‐kinase p110α (PI3K‐p110α), and phosphorated glycogen synthase kinase‐3β (Ser9) (p‐GSK3β).ConclusionThese results indicating that the SZJ‐1 and SZJ‐2 might improve the insulin resistant symptoms by improving the energy metabolic level and increasing the glycogen synthase activity of HepG2 cells.
Adaptive‐weighted high order TV algorithm for sparse‐view CT reconstruction
Authors: Xi Yarui , Zhou Pengwu , Yu Haijun , Zhang Tao , Zhang Lingli , Qiao Zhiwei , Liu Fenglin 
Published: March 2023

AbstractBackgroundWith the development of low‐dose computed tomography (CT), incomplete data reconstruction has been widely concerned. The total variation (TV) minimization algorithm can accurately reconstruct images from sparse or noisy data.PurposeHowever, the traditional TV algorithm ignores the direction of structures in images, leading to the loss of edge information and block artifacts when the object is not piecewise constant. Since the anisotropic information can facilitate preserving the edge and detail information in images, we aim to improve the TV algorithm in terms of reconstruction accuracy via this approach.MethodsIn this paper, we propose an adaptive‐weighted high order total variation (awHOTV) algorithm. We construct the second order TV‐norm using the second order gradient, adapt the anisotropic edge property between neighboring image pixels, adjust the local image‐intensity gradient to keep edge information, and design the corresponding Chambolle‐Pock (CP) solving algorithm. Implementing the proposed algorithm, comprehensive studies are conducted in the ideal projection data experiment where the Structural similarity (SSIM), Root Mean Square Error (RMSE), Contrast to noise ratio (CNR), and modulation transform function (MTF) curves are utilized to evaluate the quality of reconstructed images in statism, structure, spatial resolution, and contrast, respectively. In the noisy data experiment, we further use the noise power spectrum (NPS) curve to evaluate the reconstructed images and compare it with other three algorithms.ResultsWe use the 2D slice in the XCAT phantom, 2D slice in TCIA Challenge data and FORBILD phantom as simulation phantoms and use real bird data for real verification. The results show that, compared with the traditional TV and FBP algorithms, the awHOTV has better performance in terms of RMSE, SSIM, and Pearson correlation coefficient (PCC) under the projected data with different sparsity. In addition, the awHOTV algorithm is robust against the noise of different intensities.ConclusionsThe proposed awHOTV method can reconstruct the images with high accuracy under sparse or noisy data. The awHOTV solves the strip artifacts caused by sparse data in the FBP method. Compared with the TV method, the awHOTV can effectively suppress block artifacts and has good detail protection ability.
Influence of Environmental Variables on Catch of Walleye Broodfish for Propagation Efforts
Authors: Kowalewski Lucas K. , Parr Michael A. , Neely Ben C. 
Published: March 2023

Abstract
Walleye Stizostedion vitreum support recreational fisheries throughout their range but increasingly require annual stockings to maintain populations. In many areas of their distribution, Walleye broodfish are collected annually and manually spawned onsite before fertilized eggs are transported to fish culture facilities for rearing. As such, efficiency of these programs is reliant on effective capture of broodfish. Generalized additive models were used to explain the influence of eight environmental factors on catch per effort (CPE) of ripe female Walleye and male Walleye during brood collection events from Hillsdale Reservoir, Kansas, using data from 2009 to 2021 (excluding 2020). The ripe female model explained 63% of the deviance in CPE and identified the day of year, water temperature, moon illumination, previous autumn CPE of preferred-length fish (CPEp), and previous autumn proportional size distribution of preferred-length fish (PSDp) as influential variables. The male model explained 64% of the deviance in CPE and identified the day of year, change in barometric pressure from the previous day, water temperature, and previous autumn proportional size distribution of quality-length fish PSDq as influential variables. These findings provide useful information regarding collection of Walleye broodfish and can be leveraged to increase efficiency of future operations range-wide.
The case for basinwide passage and habitat restoration for Pacific Lamprey in the Willamette River basin (Oregon, USA)
Authors: Clemens Benjamin J. , Friesen Thomas A. , Gregory Stanley V. , Zambory Courtney L. 
Published: March 2023

Abstract

Objective
The Pacific Lamprey Entosphenus tridentatus is an anadromous fish that provides many ecosystem services and is important to Native American tribes, which harvest lamprey for cultural uses. The largest harvest of adult Pacific Lamprey in North America occurs at Willamette Falls (WF) in Oregon, USA. Lamprey have experienced conservation problems for decades, and harvest numbers at WF have plummeted approximately 33-fold from a maximum of over 500,000 during 1946 to an average of about 16,000 per year during 1969–1999. Recent harvest has dropped to less than 6,000 lamprey/year during 2000–2021. However, existing conservation plans do not provide a historical perspective on the lamprey harvest at WF and the cumulative threats in the Willamette River basin that likely caused lamprey harvest reductions. Land development, river impoundment, and water pollution coincided with significant decreases in lamprey harvest at WF. These (and other) threats have resulted in reduced habitat for larval lamprey in the basin and, thus, a reduction in the pheromones they release, which attract adults. Conservation plans do not identify specific, basinwide goals to increase the number of lamprey in general and to provide more lamprey for harvest in particular.

Methods
Therefore, in this review paper, we provide a conservation perspective on three topics to increase and monitor lamprey abundance at WF.

Result
We (1) provide an up-to-date history of the lamprey harvest and its regulation at WF; (2) recommend addressing the lack of basinwide, lamprey-specific passage and habitat restoration to improve the quantity and quality of lamprey spawning and rearing habitats; and (3) identify research needs for monitoring the lamprey population(s) at WF.

Conclusion
We conclude that lamprey-specific passage improvements and habitat restoration throughout the Willamette River basin above WF are necessary to bolster abundance.