bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anaerobutyricum hallii promotes the functional depletion of a food carcinogen in diverse healthy fecal microbiota
Authors: Ramirez Garcia Alejandro , Greppi Anna , Constancias Florentin , Ruscheweyh Hans-Joachim , Gasser Julie , Hurley Katherine , Sturla Shana J. , Schwab Clarissa , Lacroix Christophe 
Published: September 2023

IntroductionAnaerobutyricum hallii is a human gut commensal that transforms the heterocyclic amine 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP), a carcinogen from cooked meat. The transformation mechanism involves the microbial production of acrolein from glycerol, and its conjugation with PhIP, thus blocking its mutagenic potential. A potential cancer prevention strategy could therefore involve supplementing complex human microbial communities with metabolically competent bacteria such as A. hallii that can deplete PhIP. However, it has not been established how the proportion of A. hallii in diverse healthy human gut microbial communities relates to functional capacity for PhIP transformation and, moreover, how supplementing microbiomes with A. hallii affects this function.MethodsIn this study, shotgun metagenomics was used to study taxonomic profiling, the abundance of glycerol/diol dehydratase (gdh)-harboring taxa, the proportion of resident A. hallii, and the reconstruction of A. hallii population genomes in the fecal samples of 20 healthy young adult donors. Furthermore, the influence of supplementing 106 cells/mL of A. hallii DSM 3353 with diluted fecal microbiota was characterized.Results and discussionSix microbiota were assigned to Bacteroides, nine to Prevotella, and five to Ruminococcus by enterotype-associated clustering. The total number of gdh copies in the 20 fecal microbiota expressed per 1010 bacterial cells ranged between 1.32 × 108 and 1.15 × 109. Eighteen out of the 20 donors were dominated by A. hallii, representing between 33% and 94% of the total gdh relative abundance of the samples. The microbiota with low A. hallii abundance (i.e., with a relative abundance &lt; 1%) transformed less PhIP than the microbiota with high A. hallii abundance (i.e., with a relative abundance &gt; 1%). Furthermore, supplementing the low-A. hallii-abundant microbiota with glycerol significantly increased the PhIP transformation capacity after 6 h while reducing total short-chain fatty acid (SCFA) levels, which is most likely due to acrolein production. Although acetate decreased in all microbiota with glycerol and with the combination of glycerol and A. hallii, for most of the microbiomes, butyrate production increased over time. Thus, for a significant number of diverse healthy human fecal microbiomes, and especially when they have little of the taxa to start with, supplementing A. hallii increases PhIP transformation. These findings suggest the need to test in vivo whether supplementing microbiomes with A. hallii reduces PhIP exposure.
Effectiveness of a Novel Endophyte Bacillus velezensis Strain B1 in the Biocontrol of Pear Postharvest Ring Rot
Authors: Yang Suling , Song Meifang , Qi Limei , Liu Guijun , Zhou Sijing , Qiao Yuchen , Wang Ping , Gu Haike , Wu Liqin 
Published: September 2023

Pear ring rot caused by Botryosphaeria dothidea severely affects the quality and storage life of pear fruit. Plant endophytes are potential new sources of biocontrol agents due to their abilities to produce novel bioactive products. In this work, we focused on an endophytic strain B1, which was isolated from roots of Dendrobium huoshanense. Notably, strain B1 exhibited strongly inhibitory effects against the growth of Botryosphaeria dothidea with an inhibition rate of 73.2% after 1 week of co-cultivation. Furthermore, it displayed a broad-spectrum inhibitory activity. Strain B1 also effectively delayed the onset of ring rot caused by B. dothidea in pear fruit and reduced the lesion diameter by 73.4%. Strain B1 was identified as Bacillus velezensis based on core-genome phylogeny. Genome mining with antiSMASH revealed 13 potential gene clusters involved in antimicrobial metabolites. Three main groups of lipopeptides (surfactin, iturin and fengycin) were identified with MALDI-TOF-MS, and the lipopeptides in the inhibition zone were greatly upregulated by B. dothidea; especially, fengycin isoforms were detected with higher abundance. These results proved that lipopeptides produced by strain B1 had significant antagonistic effect on B. dothidea. In conclusion, our results suggest that strain B1 has potential applications in the biocontrol of B. dothidea in postharvest fruit.
Using the BWA (Bertaut-Warren-Averbach) Method to Optimize Crystalline Powders Such as LiFePO4
Authors: Bobyl Aleksandr , Konkov Oleg , Faradzheva Mislimat , Kasatkin Igor 
Published: September 2023

The average sizes L¯i, and their dispersion Wi along the i-th axis, of crystallites in powders are used to determine X-ray diffraction sizes, Di XRD, averaged over crystallite columns within the BWA method. Numerical calculations have been carried out for an orthorhombic lattice of crystallites, such as LiFePO4, NMC, having a Lamé’s g-type superellipsoid shape. For lognormal distributions, the analytical expression for the normalized coefficient Kn has been found: Kn=Di XRD/L¯i=Kg,0+KgW2, where Kg,0 is a constant at W→0, Kg is a constant depending on the g -type shape. The dependences of Di XRD are also calculated for normal distribution. A fairly simple equation can be obtained as a result of analytical transformations in the framework of experimentally validated approximations. However, a simpler way is to carry out numerical computer calculations with subsequent approximation of the calculated curves. Using the obtained analytical expressions to control technologies from nuclear fuel to cathode materials will improve the efficiency of flexible energy network, especially storage in autonomous and standby power plants.
Mechanisms of oral microbial biofilm formation in healthy people and patients with chronic generalized periodontitis
Authors: Leonteva A. V. , Pototskaya L. A. , Chervinets Y. V. 
Published: September 2023

Relevance. Oral biofilms are integrated three-dimensional symbiontic microbial structures embedded in the extracellular matrix that form not only on the mucous membrane but also on hard surfaces such as enamel and root of teeth, as well as dentures. Our study correlated biofilms and the onset of such a serious oral disease as chronic generalized periodontitis.Material and Methods. The analysis of national and international literature in PubMed, Google Search and eLIBRARY databases over the past 15 years served as material and methods.Results. There is currently an increased interest in studying the mechanisms of microbial biofilms and the factors affecting this process. Planktonic bacteria are more sensitive to antimicrobials than bacteria in biofilms. Several factors, such as gas signalling molecules, may become an important tool for intermicrobial communication in a biofilm. Biofilm microorganisms are highly resistant to antibacterial drugs. Bacteria release enzymes and toxins that stimulate the body to produce large amounts of specific antibodies and cytokines. However, the immune components are blocked by the biofilm extracellular matrix and cannot enter the biofilm. Immune complexes are formed in the infection foci, damage the body's tissues, and aggravate inflammation.Conclusion. Periodontal diseases are the most common oral diseases in humans, and their onset is closely related to the pathogenic properties of the oral microbiota, which exists in biofilms. The mechanisms of biofilm formation are complex and associated with the expression of various microorganism/matrix pathogenicity factors by oral commensal microorganisms. Further study is necessary to understand the way of biofilm formation neutralization and to find an effective treatment for chronic generalized periodontitis.
COMPARATIVE STUDY OF ROOTSTOCKS AND THEIR EFFECTS ON STIONIC ESTABLISHMENT OF THE MANGO SEEDLINGS
Authors: Kubar Ishtiaque Hussain , Memon Noor un nisa , Sharif Naseem , Memoona Islam Majeedano  , Muzamil Farooque Jamali  , Magsi Hube Ali , Abbasi Farha Feroz , Aslam Komal 
Published: September 2023

To explore the quality rootstock which impart better stionic establishment of the mango seedlings, we studied the comparative effect of scion and rootstock using three mango scion varieties (Sindhri, Chaunsa and Neelum) grafted on three rootstocks i.e. Ratam, Pado and Sindhri. Parameters including seed germination (%), seedling height (cm), leaves seedling rootstock-1, seedling vigor index (SVI), fresh biomass of shoot and roots (g), dry biomass of shoot and roots (g), chlorophyll content (rg), rootstock diameter (mm), scion diameter (mm), stionic ratio, leaves graft-1 and sturdiness quotient were studied. Results showed the best seed germination (88.33%), seedling vigor index (5158.74), and sturdiness quotient (3.89) in Pado rootstock. However maximum fresh biomass of shoot (20.67 g), fresh biomass of roots (16.2 g), dry biomass of shoot (12.65 g), dry biomass of roots (8.33 g) and scion diameter of the rootstocks were observed in Sindhri rootstocks. Regarding grafting data, the best stionic ratio (0.93) was observed from the scion and rootstock combination of Neelum and Pado rootstock. It was concluded that Pado rootstock performed better in terms of seedling rootstock and for successful stionic establishment of the mango seedlings
BIOCHEMICAL CHARACTERIZATION OF STEVIA ESSENTIAL OIL THROUGH DIFFERENT DRYING METHODS AND SOLVENTS
Authors: Ibrar Sehrish , Yousaf Najma , Habib Umer , Hussain Tanveer , Shahbaz Hassan , Ali Irfan , Naqvi Syed Muiz Mehdi , Ahmad Tanveer , Asghar Sana 
Published: September 2023

Essential extracts from plants have been used as a food and medicinal cure since ancient times. Some plants have a good portion of carbohydrates e.g., stevia is being used as an excellent alternative to synthetic compounds i.e., sugar. Therefore, it is important to chemically characterize these nutrients and study their various biological properties. This research aimed to extract essential oil from stevia plant by using Soxhlet apparatus and analysis of nutrients present in the oil were performed by high performance liquid chromatography system i.e., HPLC. Two extraction solvents i.e., hexane (60/40; v/v) and ethanol (70/30; v/v) were used to compare the concentration of bioactive compounds. The plant material was subjected to two drying methods i.e., air dry and oven dry. 10 major compounds were identified as a result of the chemical characterization, in which steviol bioside i.e., 317.01615 (m/z) and stevioside (803.2189 m/z) that is obtained from oven drying using ethanol as extraction solvent were slightly greater than steviol bioside i.e., 315.2183 (m/z) and stevioside i.e., 802.8531 (m/z) obtained from oven drying using hexane as extraction solvent. Likewise, plant material subjected to air dry with ethanol as extraction solvent resulted in greater yield of steviol bioside i.e., 317.0717 (m/z) and stevioside i.e., 803. 3179 (m/z) as compared to steviol bioside i.e., 315.1104 (m/z) and stevioside (802.3185 m/z) that is obtained by air dry using hexane as a solvent. While using fresh leaves the concentrations of compounds i.e., stevioside (799.2187 m/z), dulcoside A (787.3759 m/z) and steviol (639.4189 m/z) obtained by using ethanol were greater than concentration of compounds obtained by hexane. While Rebaudioside A (965.3213 m/z), Rebaudioside B (803.3717 m/z), Rebaudioside C (940.4276 m/z), Rebaudioside D (1066.4763 m/z), Rebaudioside E (964.4273 m/z) and Rebaudioside F (789.3857 m/z) obtained by using hexane as extraction solvent were greater as compared to concentration of compounds obtained by using ethanol. Based on these results, we can suggest that Stevia rebaudiana Bertoni can be a natural source of antioxidant, anti-inflammation and antimicrobial properties. It can also be considerd as a potential source of essential oil. These results suggest that the leaves of S. rebaudiana Bertoni can serve as therapeutic agents or in prevention of certain inflammatory diseases through their radical-scavenging capacities.
Effect of PGPR and Biogas Slurry on Growth and Yield Parameters of Pea under Salt Affected Conditions
Authors: Muslim Nafeesa , Akhtar Naseem , Saddiq Sumreen , Imran Muhammad , Yousaf Kiran , Sharif Naseem 
Published: September 2023

Salt stress is a significant abiotic plant growth restrictive factor; it is becoming a severe environmental threat. The microorganism in the rhizosphere especially fungi and bacteria can increase the plant production under stress conditions both by direct and indirect mechanisms. A field experiment was conducted to evaluate the potential of biogas slurry and plant growth promoting rhizobacteria (PGPR) at different levels of salinity to improve the growth and yield of pea (Pisum sativum ). In field experiment biogas slurry @ 600 kg ha-1 and 800 kg ha-1 and PGPR strain “bacillus subtilis” was applied along with 6 dS m-1 and 8 dS m-1 levels of salt stress in addition to recommended doses of nitrogen, potassium and phosphorus fertilizer. The results revealed that the combined application of PGPR and biogas slurry under normal soil conditions increased shoot length by 30.27% while under saline conditions it increased up-to 65.27%. Soil salinity reduced root length up-to 79.155% at 8 dS m-1 as compared to control. Application of biogas slurry improved 5.93% root length under salt stress as compared to respective control, on the other hand the combined application of PGPR and biogas slurry increased root length by 33.128% under normal conditions and under salinity stress it increased by 73.53%.Soil salinity reduced chlorophyll content 36.54% of pea decrease under salt stress, the application of biogas slurry under the same condition improved 29.26% chlorophyll content of pea but the combined application of PGPR and biogas slurry enhanced the chlorophyll contents 4.68% as compared to solely application of biogas slurry. The results clearly indicated that the combined application of PGPR and biogas slurry is the best source to enhance the growth and yield of pea under normal as well as under salinity stress.
Key Words: PGPR, Bio gas, Salinity, Peas, Growth, Yield.
GENETIC ANALYSIS IN VARIOUS GENOTYPES OF BREAD WHEAT UNDER NORMAL AND HEAT-STRESS ENVIRONMENTS
Authors: Rind Raza Ali , Memon Shabana , Jatoi Wajid Ali , Soomro Aijaz Ahmed 
Published: September 2023

Abiotic stresses have brought the crops to a destructive position towards yield production of crops, especially wheat. The present study was investigated to compare the relationship between normal and heat stress conditions under two different sowing dates viz. normal and late sowing dates (25th Nov and 25th Dec). The correlation coefficients varied with both sowing dates (normal and late planting). In normal planting number of grains showed a significant positive correlation with grain weight spike-1 (r = 0.618**), grain yield plant-1 (r = 0.591**), seed index (r =0 .456**), biological yield plant-1 (r = 0.540**) and harvest index (r =0 .667**). Grains spike-1 contributed significant positive correlation with grains spike-1 (r=0.094**), grain yield plant-1 (r=0.844**), biological yield plant-1 (r=0.936**), harvest index (r=0.556**), leaf area (r=0.791**), relative water content (r=0.763**), chlorophyll content (r=0.853**), cell membrane stability (r=0.828**) and stomatal conductance (r=0.292**). Grain yield plant-1 exhibited a significant positive correlation under normal planting with the number of tillers plant-1, number of spikelets spike-1, grains spike-1, and grain weight spike-1 (r=0.695**,0.207*,0.591**and 0.950**), respectively. Whereas, late planting declared grains spike-1 revelaed signficant positive correlation with grains spike-1 (r=0.094**), grain yield plant-1 (r=0.844**), biological yield plant-1 (r=0.936**), harvest index (r=0.556**), leaf area (r=0.791**),relative water content (r=0.763**), chlorophyll content (r=0.853**), cell membrane stability (r=0.828**) and stolatal conductance (r=0.292**). Under late planting, Yield showed a significant positive correlation with spike length, grains spike-1, and grain weight spike-1 (r=0.343**,0.844**, and 0.964**), respectively
International Quality Award Models: Innovation Enablers or Inhibitors?
Authors: Edgeman Rick , Eskildsen Jacob 
Published: September 2023

Historical development of models and criteria supporting major international quality awards is driven more by continuous or incremental improvement than by breakthrough improvement. This has been the case in particular relative the most enduring such awards - the European Foundation for Quality Management (EFQM) Business Excellence Award, America’s Baldrige National Quality Award for Performance Excellence (MBNQA), and the Shingo Prize for Operational Excellence. In possible contrast are initiatives such as the United Nations Sustainable Development Goals (UN SDGs) and the imputed urgency for improvement, innovation and change associated with these, due in large to the sheer scope and magnitude of change needed – especially with respect to societal or environmental challenges. While sufficiently rapid iteration across a large enough number of cycles has the potential of rivaling the magnitude of innovation attained through a focus on breakthrough improvement, such an approach is unlikely to precisely match the destination of innovation attained through breakthrough improvement approaches. One significant reason is that incremental improvements often target minor course corrections with well-defined trajectories, whereas breakthrough efforts target larger strides with more mystery between the point of origination and the targeted destination. Differences in these approaches impact products and services reaching the market in many ways, hence impacting their marketplace performance. Among differences of note are ones in the natures of the product or service brought to market, the timing of their market arrival, the levels of creativity and innovation required, design innovations introduced, consumer receptivity and perception of iteration vs. innovation, perceived impact, consumer perception of the company, and – not to be ignored – risk and reward elements. Questions explored herein are whether the models and criteria used by international quality award frameworks enable innovation and entre-intrapreneurship, inhibit these, or are innovation and entre-intrapreneurial neutral. Similarly, the impact of the UN SDGs in relation to innovation are discussed.
EFFICIENCY OF METHODS OF FRAME VERIFICATION IN PROFESSIONAL MOBILE RADIO COMMUNICATION SYSTEMS OF THE DMR STANDARD
Authors: Тынянкин С.И. , Балюков В.М. , Минеев В.А. , Хмельков А.Н. 
Published: September 2023

Приведены результаты исследования эффективности различных способов проверки на целостность пакетов данных, передаваемых по каналу связи с шумом, которые регламентированы международным стандартом DMR для верификации наиболее важных фрагментов заголовков пакетов, включая способ вычисления контрольной суммы (checksum).
The results of a study of the effectiveness of various methods of checking the integrity of data packets transmitted over a noise communication channel, which are regulated by the international standard DMR, for verifying the most important fragments of packet headers, including the method of calculating the checksum, are presented.
IMPLEMENTATION OF INTERREGIONAL NUMBER PORTABILITY AS ONE OF THE DIRECTIONS FOR THE MNP DEVELOPMENT
Authors: Кутепов М.Ю. , Бухарев И.А. , Плахов В.В. 
Published: September 2023

В настоящее время абонентский номер телефона фактически стал уникальным идентификатором гражданина не только как пользователя услуг связи, но и для организации и подтверждения доступа к различным сервисам (в том числе государственным), порталам, личным кабинетам, банковским счетам и приложениям. Реализация возможности перенесения абонентского номера между различными субъектами Российской Федерации позволит значительно упростить жизнь абонентам при внутренней миграции, а также окончательно отменить «мобильное рабство» в стране. В статье приводится анализ возможности внедрения бизнес-процесса межрегионального перенесения абонентского номера с указанием статистических данных, результатов соответствующего пилотного проекта, а также предложений по возможным подходам к реализации такой услуги на территории всей страны.
At present, the subscriber phone number has actually become a unique identifier of a citizen not only as a user of communication services, but also for organizing and confirming access to various services (including government ones), portals, personal accounts, bank accounts, etc. Implementation of interregional number portability in the Russian Federation will significantly simplify the subscriber’s life during internal migration as well as finally abolish the «mobile slavery» in the Russian Federation. This article presents an analysis of the possibility on implementing the interregional number portability business-process, indicating statistical data, the results of the corresponding pilot project as well as proposals on possible approaches to the implementation of such the service throughout the country.
A SWARM OF UAVs IS A NEW PARADIGM FOR THE USE OF SMALL UNMANNED AERIAL VEHICLES
Authors: Ступницкий М.М. , Мырова Л.О. , Королев П.С. 
Published: September 2023

Приведены результаты анализа особенностей характеристик роя беспилотных летательных аппаратов (БПЛА), значимые для их поражения. Рассмотрены способы формирования роя БПЛА и управления им. Приведены особенности современных БПЛА и представлена обобщенная модель роя БПЛА как системы искусственного интеллекта.
The results of the analysis of the characteristics of the characteristics of the swarm of unmanned aerial vehicles (UAV), which are significant for their destruction, are given. The methods for forming and controlling the UAV swarm are considered. The generalized model of the UAV swarm as an artificial intelligence system and the features of modern UAVs are presented.
INVESTIGATION OF NOISE IMMUNITY OF NON-ORTHOGONAL MULTIPLE ACCESS SYSTEMS WITH PATTERN DIVISION
Authors: Бакулин М.Г. , Режеб Т.Б.К.Б. , Крейнделин В.Б. , Панкратов Д.Ю. , Смирнов А.Э. 
Published: September 2023

Развитие новых сценариев использования систем беспроводной связи таких, как интернет вещей и межмашинная связь М2М, повышает требования к ёмкости сети связи. К сожалению, потенциальные возможности методов ортогонального множественного доступа (OMA, Orthogonal Multiple Access) практически исчерпаны в технологиях сотовой связи, предшествующих пятому поколению (5G). Для улучшения качественных показателей систем связи предлагается использование методов неортогонального множественного доступа (NOMA, Non-Orthogonal Multiple Access). В данной работе проведено исследование методов неортогонального множественного доступа с разделением по шаблону (PDMA, Pattern Division Multiple Access) как на восходящей, так и на нисходящей линии системы связи. Приведено математическое описание системы PDMA, а также даны результаты помехоустойчивости на восходящей и нисходящей линиях.
The development of new wireless communication scenarios such as the Internet of Things and Machine-To-Machine increases demands on communications network capacity. Unfortunately, the potential capabilities of Orthogonal Multiple Access (OMA) methods are almost exhausted in cellular communication technologies prior to 5G technology. To improve the quality of communication systems the use of Non-Orthogonal Multiple Access (NOMA) is proposed. In this paper, a study of Pattern Division Multiple Access (PDMA) methods on both uplink and downlink communication systems was conducted. A mathematical description of the PDMA system is given, as well as noise immunity results on the uplink and downlink.