bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetically determined thyroid disease and risk of preeclampsia : a Mendelian Randomization study
Authors: Zhang Zhan , Li Jianfeng , Angwen Chenglinlongzhu , Li Zhongwei , Wang Bowen , Wang Rong , Wang Xin , Liu Duo , Xie Ping 
Published: September 2023

Abstract
Background: Observational studies suggest a possible correlation between thyroid disease and preeclampsia. However, the causal relationship between them is unclear.
OBJECTIVE: The purpose of this research was to examine at the potential links between thyroid illness and preeclampsia.
METHODS: A two-sample Mendelian randomization (MR) research was conducted using data from a genome-wide association analysis to investigate the probable causal influence of thyroid disease and preeclampsia. In this study, the inverse variance weighted (IVW) method was used as the primary result.
RESULTS: The results of the study showed that hypothyroidism (OR=3.804, 95% CI: 1.121-12.911; p=0.0321), thyroid stimulating hormone (TSH) (OR=1.128, 95% CI: 1.009-1.260; p=0.0340) were positively associated with preeclampsia. No significant association was found between genetic predisposition to hyperthyroidism and preeclampsia.
CONCLUSION: This MR study found a positive association between genetically predicted hypothyroidism, TSH and preeclampsia.
Single-centre epidemiological investigation and diagnosis analysis of osteonecrosis of the femoral head
Authors: Liu Wangyan , Yue Ju’an , Guo Xiaozhong , Wang Randong , Li Bing , Sun Qiang , Chen Jiao , Zhao Fengnian , Fu Hao 
Published: September 2023

Abstract
Purpose
In this retrospective case investigation, we analysed the data of patients with osteonecrosis of the femoral head (ONFH) in a single centre to reveal demographic and clinical diagnostic features of ONFH and provide a reference for its prevention, diagnosis, and treatment.
Methods
We collected data from patients in our hospital, focusing on the aetiology and diagnosis of ONFH. Medical records and self-design questionnaires were used to collect information for statistical analysis, including age, aetiology, reason for glucocorticoid use, hospital level at first visit, and diagnosis.
Results
In total, 203 patients with complete medical records were included in the analysis. The mean patient age was 41.23 ± 12.61 years. The peak age distribution was in the 30s among men and in the 40s among women. Of the total, 100 patients had steroid-induced ONFH (49.26%), 55 had alcoholic ONFH (27.09%), 23 had traumatic ONFH (11.33%), and 25 had idiopathic ONFH (12.32%). Forty-seven patients had intakes of ≥ 2 g prednisone or its equivalent within a 3-month period, and fifty-three patients had a history of low-dose steroid in the past 3 years. Of the total, 112 patients were diagnosed with ONFH at the first visit (diagnosis rate55.17%). The rate of misdiagnosis at all hospital levels was highest for lumbar disc herniation.
Conclusions
Most patients with ONFH were middle-aged and had steroid-induced ONFH. The misdiagnosis rate of ONFH at the first visit was very high, especially for misdiagnosis of lumbar disc herniation, indicating that the diagnosis of ONFH must be improved.
Bacteraemia-associated Hypertensive Response, a Case (Report) for the Inflammation/hypertension Axis
Authors: Russwurm Martin , Hoyer Joachim 
Published: September 2023

Abstract

Background:
Hypertensive crises are life-threatening conditions that can be associated with organ damage including acute heart failure, intracerebral haemorrhage, seizures and coma and are common in dialysis patients.
Case Presentation:
We report a case of a sudden onset of repeated episodes of hypertensive crises in a young dialysis patient. Surprisingly, we found a catheter-associated Pseudomonas aeruginosa-infected right atrial thrombus and smouldering endocarditis to be the cause for these exacerbations in blood pressure. Anti-hypertensive therapy did not suffice to control critical surges of blood pressure particularly at dialysis. An anti-infective approach comprising antibiotics, catheter removal and finally extraction of the thrombus by open-heart surgery led to resolution of severe hypertension.
Conclusions:
This case contradicts canonical assumptions that severe infections with bacteraemia unambiguously lead to hypotension introducing the septic vicious cycle. The specific characteristics of the case (remitting hypertensive crisis while the thrombus experienced shear stress at dialysis, supposedly leading to bacterial antigen flooding and resolution of hypertension after thrombus removal) support the hypothesis that infection was the decisive cause of severe hypertension here. With that, albeit naturally anecdotally, this case might serve as a hint that infection can be causative for hypertension or amplifying it. Thus, this case emerges as premier direct evidence for the substantiation of the increasingly well-established hypertension/inflammation axis.
Colonial Microcystis’ biomass affects its shift to attached diatom dominance under aeration mixing
Authors: Wang Xiaodong , Che Xuan , Zhou Jian , Qin Boqiang , Tang Xiangming , Liu Ziqiu , Liu Xingguo 
Published: September 2023

Abstract
The effect of hydrodynamic mixing on controlling Microcystis blooms or changing the algal community to diatom dominance has been widely studied; however, the effects of colonial Microcystis biomass on the development of the algal community are poorly known. Here, in order to study the changes in Microcystis blooms under continuous aeration mixing, an experiment was carried out in a greenhouse with factors of varying biomass of Microcystis and inorganic nitrogen and phosphorus enrichment in summer. There were three chlorophyll a (Chl-a) levels in six treatments: low Chl-a level of 68.4 µg L− 1 (L, L-E), medium Chl-a level of 468.7 µg L− 1 (M, M-E), and high Chl-a level of 924.1 µg L− 1 (H, H-E). Treatments L-E, M-E and H-E were enriched with the same inorganic nitrogen and phosphorus nutrients. During the experiment of 30 days, the concentration of Microcystis and Chl-a decreased, and diatom Nitzschia palea cells appeared in all the treatments, which became dominant in treatments M, M-E, H and H-E, with the highest biomass of 9.41 ± 1.96 mg L− 1 Nitzschia in treatment H-E on day 30. The rank order of the biomass of Nitzschia from low to high was (L = L-E) < (M = M-E) < H < H-E (P < 0.05). In addition, N. palea cells were aggregates attached to Microcystis colonies in all the treatments. The results showed that the initial biomass of colonial Microcystis affected the algal shift from Microcystis dominance to Nitzschia dominance. However, the enriched inorganic nitrogen and phosphorus was not so important for the algal shift. The shift from Microcystis dominance to diatom dominance under continuous aeration mixing may be caused by low light conditions as well as the nutrients. And the aerobic condition caused by aeration mixing maintained the colonial mucilaginous sheath to support the growth of Nitzschia cells in aggregation.
Combining Neural Networks, UMAP and DBM Clustering to Identify Cell Populations Accurately, Quickly and Easily in Mass and Fluorescence Cytometry
Authors: Meehan Connor , Meehan Stephen , Ebrahimian Jonathan , Moore Wayne , Parks David , Walther Guenther , Herzenberg Leonore 
Published: September 2023

Abstract
Identifying cell populations in flow cytometry data is mostly done via a “manual gating” method that often lacks verifiability and reproducibility, even in the hands of experienced investigators. In 2018, flowLearn outperformed the previous best automatic gating methods by demonstrating near perfect identification of known populations on two fluorescence cytometry datasets. Then LDA exemplified similar accuracy on five mass cytometry datasets, but with much easier dataset training. However, for optimal results, LDA requires extensive manual gating on the samples it classifies with that training.
Here, we introduce an easily trainable multilayer perceptron (MLP) neural network for automatic gating. Compared to LDA, FlowSOM and PhenoGraph on three mass and six fluorescence cytometry datasets, MLP is most accurate by a significant margin, tied with LDA as the fastest and uniquely able to replace manual gating except for training purposes. Furthermore, combining MLP with UMAP’s guided dimensionality reduction feature and DBM’s clustering effectively detects new populations.
Synthesis, structures, luminescent and color properties of polyethylmethacrylate (PEMA) polymers doped with rare earths
Authors: Aktaş Demet Kaya , Buhari Thami , Erdem Murat , Eryürek Gönül 
Published: September 2023

Abstract

Solid-state bulk polymer hosts doped with rare earths are expected to play a central role in the development of future light-emitting diodes and photonic devices due to the superior properties of polymers. In this study, linear polyethylmethacrylate (PEMA) and crosslinked PEMA gels doped with CdNb2O6: Er3+/Yb3+ nanocrystal powders were synthesized by free-radical crosslinking copolymerization. Er3+/Yb3+ ion structures embedded in PEMA polymers were investigated by the X-ray diffraction technique. The average crystalline particle sizes were found using the Pielazsek particle distribution and the Scherrer equation. The particle sizes decreased from 60 nm to 17 nm for CdNb2O6: Er3+/Yb3+ powders when they were embedded in PEMA polymers. Transmission electron microscopy and scanning electron microscopy monitored the change in the morphology of polymer samples doped with CdNb2O6: Er3+/Yb3+ powders. The optical properties of polymer samples were investigated by measuring luminescence spectra at room temperature. The doped Er3+/Yb3+ ion excited with a 975 nm diode laser achieved the up-conversion (UC) emissions by involving two-photon absorptions in the visible region. The UC intensities and absorbed photon numbers were increased by increasing crosslinker content. The effects of change in the structure and morphology of the host polymer material on the color tuning, color coordinates and color quality parameters were observed.
Analysis of the Restoration Effect of Soil Seed Bank on Waste Mining Land
Authors: Zhao Xinfeng , Hailiang XU , Jia WANG , Aili Aishajiang 
Published: September 2023

Abstract
Aims
Many studies have analyzed the reasons why it is difficult to plant plants in local mining areas, revealing the restoration effect of artificial replanting measures in abandoned mining areas. It is remains unclear if this restoration effect will continue or only be reflected within 1–2 years after repair.
Methods
This study used continuous 10 years of field monitoring to answer this question by analyzing the restoration effect of seed supplementation on abandoned mining areas.
Results
(1) In the second year after seed-supplement, the plant growth indicators (vegetation coverage, vegetation height, plant density, aboveground biomass) and plant species diversity (species richness, Shannon-Wiener, Simpson, and Pielou) under the commercial grass seed treatment (mode A) and the soil seed bank treatment (mode B) are significantly higher than that of the CK (P < 0.01). (2) In the 7th year after seed-supplement, the above indicators of plant growth in mode B are respectively significantly higher than those in mode A in sequence. In mode B, in soil layer of 0 ~ 10 cm, 10 ~ 20 cm, 20 ~ 30 cm, 30 ~ 40 cm and 40 ~ 50 cm, the soil-stone ratio are respectively 2.53, 11.31, 22.37, 21.77 and 11.19 times higher than that of the CK; And the soil bulk density are respectively decreased by 40.15%, 20.09%, 25.58% and 10.20% compared with the CK. (3) There is a significant positive correlation between plant growth and soil-stone ratio (P < 0.01).
Conclusions
It is not ideal of actual restore effect when commercial seeds are used in compared to soil seed bank.
Shaping Carbon into Carbon matrices with Tuneable Diameter and Photo luminescent Properties
Authors: Torre Alessandro La 
Published: September 2023

Abstract
We report on a new form of nanoscale carbon in the shape of hemispherical carbon matrices and with the bonding characteristics of either sp1 or sp2 carbon. The carbon matrices are formed spontaneously at high temperature from organic molecules adsorbed on the surface of colloidal nanoparticles, with the metal core acting simultaneously as catalyst and template for the carbon matrices growth. This new method of synthesis has demonstrated unprecedented levels of control over the carbon matrices formation, as thermally driven Ostwald ripening of the metal core provides for precise control of the carbon matrices diameter, whilst the propensity of metal nanoparticles to self-assemble into super-lattices enables the formation of highly ordered hexagonal arrays of the carbon matrices on different types of surfaces. Significantly, this new form of carbon is optically active. We have demonstrated experimentally that photoluminescence properties of carbon matrices confirmed the theoretical prediction for carbyne species.
Exosomes mediate mitochondria dynamic and metabolic reprogramming for periodontal bone homeostasis
Authors: Xie Yunyi , Fu Chuanqiang , Peng Yingying , Cheng Zhilan , Niu Qingru , Yu Le , Yang Shuqing , Lin Chuanmiao , Tang Deqian , Liu Manqing , Wang Yan , Ou Qianmin 
Published: September 2023

Abstract
Background
The crosstalk between periodontal ligament stem cells (PDLSCs) and macrophages plays an important role in periodontal bone homeostasis. Metabolic reprogramming is necessary for osteoclastic differentiation of macrophages. However, whether PDLSCs exert immunomodulatory function via modulating the metabolic reprogramming of macrophages is unknown.
Methods
PDLSCs from healthy individuals (H-PDLSCs) and patients with periodontitis (I-PDLSCs) were collected, then the exosomes were respectively isolated (H-Exo, I-Exo). The functions of H-Exo and I-Exo on the osteoclast function and periodontitis treatment were compared. The molecular mechanism of H-Exo on periodontitis was detected by microRNA sequence. And the metabolic reprogramming of macrophages was analyzed by seahorse test and 13C-glucose tracer.
Results
The results indicated that H-Exo inhibited osteoclastic differentiation and bone resorption in vitro and in vivo, while I-Exo has no obvious inhibitory effects. miRNA sequencing revealed that miR-92a-3p was a key molecule involved in the immunomodulatory effects of H-Exo. H-Exo modulates mitochondrial dynamics and cellular metabolism of macrophages via the miR-92a-3p/MFN1/PKM2 axis.
Conclusions
This study offers valuable insight into the crosstalk between PDLSCs and macrophages in periodontal bone homeostasis. In addition, this study also confirms that Exo from PDLSCs can modulate macrophage mitochondria dynamic and metabolism, which is a new way for PDLSCs to exert its immunoregulatory function.
Application of Micro-computed Tomography in the Microstructure of Cervical Uncinate Processes Trabecular Bone
Authors: Hao Yunteng , Ma Yuan , Zhang Shaojie , Wang Chaoqun , Wang Wei , Li Xiaohe , Gao Shang , Li Kun , Chen Jie , Wang Haiyan , yang Yang , Gao Mingjie , Wang Jian , Li Zhijun , Shi Jun , Wang Xing 
Published: September 2023

Abstract
This study aimed to observe the distribution and morphological characteristics of the trabecular bone in the uncinate process of the cervical spine on micro-computed tomography (Micro-CT) to identify the morphological characteristics of the microstructure in the uncinate process. Micro-CT scans were performed on 31 sets of C3-C7 vertebrae comprising 155 complete bone specimens. The measurement software of the Micro-CT system was used to identify the relevant parameters of the trabecular bone in the uncinate process. Trabecular bone in the uncinate process was mainly crisscrossed longitudinally and was continuous with the trabecular bone in the vertebral body. There was a significant difference in the bone surface area between the left and right sides (P < .05); however, there were no other significant differences between the sides (P > .05). Also, there was no obvious regularity in the change with the increase in vertebral sequence number. The peaks of each parameter were mostly concentrated in C4-C6, and there were significant differences between C3 and other cervical vertebrae for other parameters (P < .05); differences between other vertebrae were not obvious. Micro-CT can be used to observe the arrangement of trabecular bone in the uncinate process of the cervical spine and allows the measurement of morphometric parameters. The trabecular bone in the uncinate process does not significantly differ between sides and vertebrae in adults.
The expression profile analysis of high-throughput lncRNA sequencing in preterm brain tissue induced by intrauterine infection/inflammation
Authors: Xie Yixuan , Ye Zhiyi , Zhou Jingqian , Yuan Tianming 
Published: September 2023

Abstract
Background
Intrauterine infection, is one of the important causes of brain injury in preterm infants. Long noncoding RNA (lncRNA) is a class of RNA molecules defined as being > 200 nucleotides in length which play a key role in brain development. In this study, we investigate the role of lncRNAs in a rat model of preterm brain tissue induced by intrauterine infection/inflammation.
Method
We divided the rats into the intrauterine infection/inflammation group and the control group then collected brain tissues from fetal or newborn rats at E17, E21, D1 and D3, respectively. High-throughput sequencing was used to analyze differentially expressed lncRNAs in brain tissue from rats and utilized bioinformatics analyzes to investigate the potential functions of these differentially expressed lncRNAs. Finally, to validated the lncRNA-sequencing results were reliable and explore the timeliness changes, we selected lncRNAs for quantitative real-time PCR (qRT-PCR) analysis at each point of time.
Result
A total of 377 differentially expressed lncRNAs were detected in brain tissue at E17, including 189 were upregulated and 188 were downregulated. Then detected 317 differentially expressed lncRNAs at D1, among which 168 were upregulated and 149 were downregulated. Moreover, the expression levels of selected lncRNAs were identified by qRT-PCR, demonstrating that the expression levels were consistent with the high-throughput sequencing results and has time-space specificity.
Conclusion
This study demonstrated the profile of lncRNA expression in brain tissue induced by intrauterine infection/inflammation was different from that in the normal control. Then, provides valuable data and new insights into the biological mechanisms of preterm brain injury.
Phenotypic age and biological age mediate the association between Life's Essential 8 and diabetic retinopathy among US adults
Authors: Wang Jia , Jin Ming-rui , Li Yu-jing , Li Xiao-xuan , Sun Yu-xin , Xiang Shuai , Zhang Meng-qi , Li Xiang-jun , Liu Wen , Qiu Zhenkang 
Published: September 2023

Abstract
Background
Cardiovascular disease (CVD) is closely associated with Diabetic retinopathy (DR), and the prevalence of DR is steadily increasing. Life's Essential 8 (LE8), a recently updated assessment of cardiovascular health (CVH), was examined in this study to determine if it was related to diabetes among adults in the United States.
Methods
316 DR participants and 2,314 non-DR participants were included in the analyses using data from the National Health and Nutrition Examination Survey (NHANES) database (2005–2018). LE8 scores, derived from eight individual metrics, were categorized into three groups: low (0–49), moderate (50–74), and high (75–100). Combining certain biomarker sets allowed for the measurement of both phenotypic age (PA) and biological age (BA). In order to evaluate the relationship between LE8 scores and DR, weighted logistic regression and linear analysis were used. The mediating function of PA and BA was investigated by mediation analysis.
Results
After adjusting for all covariates, higher LE8 scores were associated with a lower risk of DR (odds ratio [OR], 0.49; 95% confidence interval [CI], 0.26–0.93; P for trend = 0.027). A 10-score increase in LE8 score was related to a 17% decrease in the risk of DR (multivariate-adjusted OR = 0.83, 95% CI: 0.73–0.95). Mediation analysis indicated that PA and BA partially mediated 47.57% and 28.11% of the association between LE8 scores and DR, respectively.
Conclusion
A negative association was found between LE8 scores and the presence of DR, possibly mediated by PA and BA. As a result of these findings, encouraging adherence to optimal CVH levels may be beneficial in reducing the prevalence of DR.
Coexistence of IgG4-Related Hypertrophic Pachymeningitis and Anti-NMDA Receptor Encephalitis: A Rare Clinical Presentation
Authors: Tan Hu , Lan Huan , Ning Lidong , Wei Chengcong , Zhou Zhiyu 
Published: September 2023

Abstract
We present a case study involving a 53-year-old male patient exhibiting a unique combination of IgG4-related hypertrophic pachymeningitis (IgG4-RHP) and anti-NMDA receptor encephalitis. The patient's clinical manifestations, characteristics, and laboratory findings collectively showcased features characteristic of both IgG4-RHP and anti-NMDA receptor encephalitis. The patient's clinical course was marked by a diverse array of symptoms, encompassing progressive visual impairment, recurrent psychiatric and behavioral disruptions, left upper limb weakness, and radiological indications of diffuse dural thickening with prominent enhancement, coupled with diffuse cerebral edema. During the acute phase, the patient presented with a mildly elevated serum IgG4 level, and cerebrospinal fluid (CSF) analysis yielded positive results for NMDA antibodies. Histopathological assessment of the pachymeninges revealed fibroblastic proliferation accompanied by localized lymphocytic infiltration. Treatment involving corticosteroid administration led to significant alleviation of the patient's clinical symptoms and MRI-detected abnormalities. To our best knowledge, there are no analogous instances documented in existing literature. Further clinical investigations and neuropathological studies are imperative to elucidate the underlying mechanisms and determine optimal therapeutic strategies for managing this distinctive coexisting condition.
Clinical variables influencing the perception of fatigue in people with Multiple Sclerosis: a cross-sectional study using FSIQ-RMS
Authors: Sellitto Giovanni , Ruotolo Ilaria , Ianniello Antonio , Felicetti Federica , D’Ambrosi Giorgia , Berardi Anna , Galeoto Giovanni , Conte Antonella , Pozzilli Carlo 
Published: September 2023

Abstract

Background
Physical fatigue is one of the most disabling symptoms in people with Multiple Sclerosis (PwMS). Several factors might influence the development of fatigue, such as gender, education, body mass index (BMI), Expanded Disability Status Scale (EDSS), disease duration, working status (Ws), physiotherapy (Ph), and disease-modifying therapies (DMTs). Fatigue Symptoms and Impacts Questionnaire-Relapsing Multiple Sclerosis (FSIQ-RMS) is a patient-reported outcome (PRO) that allows one to define the impact of fatigue in PwMS clearly. This study aimed to assess fatigue impact on PwMS by using FSIQ-RMS.
Methods
The participants were enrolled from May to July 2021 in MS Centers of Sant'Andrea Hospital and Policlinico Umberto I Hospital in Rome. Fatigue was evaluated using the FSIQ-RMS, validated, and culturally adapted in Italian. Clinical and demographic data were collected at the same time.
Results
We enrolled 178 PwMS [Female 74.16%; RMS 82.58%, SPMS 17.52%]. FSIQ-RMS scores were significantly correlated with EDSS (p-value < 0.01). Analysis of variance between means showed a statistically significant difference between the BMI groups at the 24hours_FSIQ-RMS score and the 7days_FSIQ-RMS score (p < 0.01), with the lower BMI group having the highest scores. Furthermore, perceived fatigue significantly improved both in subjects performing Ph (p < 0.05) and in those who actively work (p < 0.01).
Conclusions
The use of FSIQ-RMS in a real-world setting confirmed that underweight and high levels of disability are closely related to fatigue. In addition, Ph and active Ws are strongly correlated with fatigue in PwMS.