bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rice straw to biofertilizer formulations: Fostering waste management for circular economy
Authors: Sharma Apurav , Soni Raman , Soni Sanjeev Kumar 
Published: May 2023

Abstract
The increasing recognition of issues related to the production of rice straw has spurred global interest, particularly in India, to reclaim rice straw generated during cultivation to mitigate the pollution. The present study focuses on the zero waste principle, which promotes long-term sustainable socio-economic and environmental benefits through the conversion of rice straw into biofertilizers. The 8-day process involves hydrolyzing delignified rice straw using Aspergillus niger P-19 capable of producing multiple hydrolytic enzymes while Klebsiella pneumoniae AP-407 grows in the nutrients present in the hydrolysate, providing plant growth-promoting traits. This method produces liquid and carrier biofertilizer formulations from a single process with a high microbial population and is economically attractive as it can be achieved in a single fermenter vessel without requiring external enzyme loading or carrier substances. This sustainable and economically feasible solution for rice straw management follows the basic principles of the circular economy.
Relationship Between Medical Personnels' Technical Behavior Attributes and Evaluation of Information Integration of Public Health Service Platform
Authors: Wu Zijian , Trigo Virginia , Zhang Manli 
Published: May 2023

Abstract
Background
The medical service cooperation model based on the regional public health information platform constructed with intelligent computing technology, thanks to the realization of information integration, promotes the sharing of cross-regional medical resources and business cooperation. In addition, through the intelligent calculation and analysis technology of integrated data, more accurate, specific, scientific and timely medical services can be offered to regional residents. Relevant studies have shown that differences in user personality attributes will affect user technology adoption and application behavior. Purpose: To explore whether Personal Innovativeness In Technology (PIIT) and Technology Characteristics Perception (TCP) affect individual cognition and evaluation of innovative technology. Method: This study selected doctors from hospitals in Guangdong Province that have established regional medical data platforms as the survey respondents. A total of 220 questionnaires were distributed to evaluate their PIIT and TCP levels and understand their cognition and evaluation of information integration. Results: Both PIIT and TCP play a positive affection on public health information integration.
Undercarboxylated OCN promotes chondrogenesis of brown adipose-derived mesenchymal stem cells through Gprc6a
Authors: Du zhangzhen , Zhang Ke , Wang Yan , Fan Shuli 
Published: May 2023

Abstract
Background: The incidence of articular cartilage defects has significantly increased over the past few decades. Stem cell therapy treatment shows promise as an adjuvant to existing cartilage restoration techniques with improved short-term clinical outcomes and radiographic health of cartilage. Improving the ability of chondrogenesis of seed cells is an important link in the treatment of cartilage defects by tissue engineering. This study examined osteocalcin (OCN) changes during brown adipose stem cells (B-ADSCs) chondrogenesis.
Methods: Brown adipose tissue was harvested from the middle of two scapulae of three days old neonatal C57B6NL mice. qRT-PCR, Western blot, and immunochemistry were used to assess differences in chondrogenesis markers. siRNA Gprc6a knockdown was used to assess whether undercarboxylated OCN (ucOCN) promotes chondrogenesis of B-ADSCs through Gprc6a.
Results: Our study verified that B-ADSCs could secrete ucOCN during chondrogenesis. Also, ucOCN, but not carboxylated OCN (cOCN), could prominently promote the expression levels of chondrogenesis markers (SOX9, COL2a1, and ACAN). Mechanistically, ucOCN promoted chondrogenesis of B-ADSCs through Gprc6a.
Conclusions: To sum up, ucOCN could promote chondrogenic differentiation of brown ADSCs through Gprc6a, which may provide a new strategy for applying stem cells in cartilage defect repair.
A New Residual Subsidence Prediction Method of Short Working Face Goaf for Safety Construction of Urban Viaduct
Authors: Li Huaizhan , Zhou Huaan , Guo Guangli , Dai Guangli , Liu Chao , Huang Cheng , Du Qiu , Yuan Yafei 
Published: May 2023

Abstract
The planned viaduct in Jining, Shandong is a priority project in the city, but the 63 working face of a mine in Jining is only 3m away from the planned viaduct, which poses the serios threat to safety construction of the viaduct. Therefore, it is necessary to evaluate the stability of the planned viaduct goaf area under the influence of 63 working face. However, the 63 working face is a short face, and the corresponding prediction of surface residual subsidence is lacking. For this purpose, the theoretical analysis and numerical simulation were employed in this paper to reveal the foundation deformation mechanism and characteristics of fractured rock and soil mass of short goaf. It was concluded that the main source of residual subsidence was the stripping and yield softening of the coal wall on both sides of short goaf. Then, the residual subsidence prediction method of short goaf was proposed for viaduct mined-out area. The new method was applied to the planned viaduct of Jining, and the effectiveness of the method was proved by InSAR and leveling monitoring results. The research results provided the technical support for viaduct construction under the influence of underground mining.
Anti-neutrophil cytoplasmic antibodies contribute to airway inflammation via induction of neutrophil extracellular traps in children with bronchiolitis obliterans
Authors: Chen Xiaowen , Wu Shangzhi , Liu Zhenwei , Huang Zhanhang , Xu Jiaxing , Wu Zhongji , Li Hui , Li Hongwei , Chen Dehui 
Published: May 2023

Abstract
Objective: It was found that the level of anti-neutrophil cytoplasmic antibodies (ANCA) in children with bronchiolitis obliterans (BO) was increased and was related to the severity of the disease previously. This study aims to explore the mechanism of ANCA in the process of BO.
Methods: ANCA and components of neutrophil extracellular traps (NETs) were detected in plasma from patients with BO (n=40) and age- and sex-matched healthy children (n=11). Plasma IgG was extracted from ANCA-positive BO children and normal controls to stimulate neutrophils, and the production of reactive oxygen species (ROS) and NETs were detected. Small airway epithelial cells (SAECs) were treated directly with NETs of various concentrations. Cell viability was determined by CCK8, and secreted inflammatory cytokines were detected by ELISA. The IgG treated neutrophils were co-cultured with SAECs and the levels of cytokines in the culture supernatants were detected by ELISA.
Results: The levels of ANCA and NETs components including dsDNA, neutrophil elastase (NE) and myeloperoxidase (MPO) in the plasma of BO children were significantly higher than those of healthy controls. ANCA-positive IgG induced neutrophils produce ROS and NETs. The cell viability of SAECs was significantly reduced upon treatment with NETs in a concentration-dependent manner. The levels of IL-8, IL-17, TNF-α and TGF-β secreted by SAECs treated with NETs were increased significantly, and the degree of increase was positively correlated with the concentration of NETs. The co-culture of neutrophils stimulated by ANCA IgG with SAECs significantly increased the expression of cytokines including IL-8, IL-17, TNF-α and TGF-β.
Conclusions: NETs induced by ANCA may exacerbate airway inflammation in children with BO.
Experiences of Boosting Inpatient Exercise After Hip Fracture Surgery Using an Alternative Workforce - A Qualitative Study
Authors: Lau Benny Ka Fai , March Marie K. , Harmer Alison R. , Caruana Sarah , Mahony Christopher , Dennis Sarah 
Published: May 2023

Abstract
Background
Thrice-daily physiotherapy immediately following surgical repair of hip fracture has been shown to be safe and to reduce total hospital length of stay. However, implementing this is challenging with respect to health service funding and staffing. A novel approach may be to utilize an alternative workforce (allied health staff and student physiotherapists) to deliver two of the three daily treatments. However, how patients and staff may view such an approach is unknown. Thus, the aim of this qualitative study was to explore the views of inpatients with surgical repair of a hip fracture, their carers, health care professionals, and physiotherapy students about the implementation and acceptability of thrice-daily physiotherapy, with two sessions delivered by the alternative workforce (the BOOST study).
Methods
Semi-structured interviews and focus groups with patients, carers, health professionals and physiotherapy students. All interviews were digitally recorded and transcribed verbatim. The transcripts were coded, and the data analysed via inductive thematic analysis.
Results
A total of 32 interviews were analysed. Five main themes were identified: (1) individual perceptions of the intervention: inpatients/carer/staff/student, (2) implementation within the service and organisational context, (3) implementation strategies that were effective, (4) improvements to implementation strategies/barriers to implementation/unsuccessful strategies and (5) future directions of BOOST.
Conclusions
The qualitative data revealed that higher frequency physiotherapy was well-received by inpatients and that staff/students involved in providing care perceived it as a safe, acceptable and valuable practice. Implementation of higher daily frequency of physiotherapy using an alternative workforce may feasibly be adopted for inpatients following hip fracture surgery.
Trial registration:
This study was approved by the Human Research Ethics Committee (HREC) of the Western Sydney Local Health District (2020/ETH02718). Mutual recognition of approval was subsequently obtained from Northern Sydney Local Health District HREC.
Time, momentum, and energy resolved pump-probe tunneling spectroscopy of two-dimensional electron systems
Authors: Ashoori Raymond , Yoo Heun Mo , Korkusinski Marek , Miravet Daniel , Baldwin Kirk , West Kenneth , Pfeiffer Loren , Hawrylak Pawel 
Published: May 2023

Abstract
Real-time probing of electrons can uncover intricate relaxation mechanisms and many-body interactions hidden in strongly correlated materials. While experimenters have used ultrafast optical pump-probe methods in bulk materials, laser heating and insensitivity below the surface prevent their application to encapsulated low-dimensional electron systems at millikelvin temperatures, home to numerous intriguing electronic phases. Here, we introduce time, momentum, and energy resolved pump-probe tunneling spectroscopy (Tr-MERTS). The method allows the injection of electrons at particular energies and observation of their subsequent decay in energy-momentum space. Using Tr-MERTS, we visualize electronic decay processes in Landau levels with lifetimes up to tens of microseconds. Although most observed features agree with simple energy-relaxation, we discover an unexpected splitting in the nonequilibrium energy spectrum in the vicinity of a ferromagnetic state. An exact diagonalization study of the system suggests that the splitting arises from a maximally spin-polarized higher energy state, distinct from a conventional equilibrium skyrmion. Furthermore, we observe time-dependent relaxation of the splitting, which we attribute to single-flipped spins forming topological spin textures. These results establish Tr-MERTS as a powerful tool for studying the dynamics and properties of a two-dimensional electronic system beyond equilibrium.
Unveiling the Potential of BaCe 0.55 Zr 0.3 Y 0.15 O 3-δ - Nd 1.5 Ba 1.5 CoFeMnO 9−δ (BCZY-NBCFM) Composite Cathodes for Improved IT-SOFC Efficiency
Authors: Ullah Abid , Hussain Basharat 
Published: May 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Prevention of ventilation-associated pneumonia applying a functionalized endotracheal tube and photodynamic therapy: a proof of principle with an artificial intubation model
Authors: Zangirolami Amanda Cristina , Pingueiro Leandro Serillo , Rodrigues Fábio M. S. , Arnaut Luis G. , Pereira Mariette M. , Bagnato Vanderlei Salvador , Blanco Kate Cristina 
Published: May 2023

Abstract
Background
Biofilm in medical devices occurs with bacterial adherence through virulence factors by a favorable environment for their proliferation. The transfer of microbial cells from biofilm in endotracheal tube to lungs increases the chances of developing severe infections. This study aimed to optimize antimicrobial photodynamic therapy (PDT) by applying a curcumin-functionalized endotracheal tube in an artificial respiratory system avoiding bacterial and their dispersion in the respiratory system, adding a mechanical ventilator.
Methods
This model was built containing three components representing oropharynx, trachea, and lungs. ET-curc was inserted into the system's trachea followed by biofilm formation.
Results
Microbial migration from Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) biofilms to the left and right lungs were evaluated with and without mechanical ventilation. PDT was applied to ET-curc using a laser (450nm) and resulted in a total bacterial inactivation, avoiding microbial flow relationships from the upper to the lower air system. The effects indicated high effectiveness in bacterial biofilm inactivation and, consequently, in the prevention of their colonization in lungs.
Conclusion
The results indicated that PDT can be an excellent alternative to prevent the spread of infectious lung diseases by multidrug-resistant microorganisms in patients under mechanical ventilation and provide conditions for starting animal model experiments.
The Effect of Funneliformis mosseae and Biochar Against Verticillium dahliae in Pepper Plants under Salt Stress
Authors: GÜNEŞ Hasret , Demir Semra , DURAK Emre DEMİRER , Boyno Gökhan 
Published: May 2023

Abstract
Stress factors, both biotic and abiotic, serve a significant role in impairing the growth and productivity of many crops. This study investigated the effects of some enzyme activities, salt scale, disease severity, plant growth, and physiological characteristics of arbuscular mycorrhizal (AM) fungi biochar (Bc) against Verticillium dahliae (Vd) in pepper plants grown under salt stress. In this study, 2% biochar, Funneliformis mosseae (Fm), Vd and different concentrations (0 mM, 50 mM, 100 mM, 150 mM) of salt were applied to pepper. It was determined that the interaction of Fm and 2% Bc significantly increased the morphological parameters of the plant, and the plant tolerated salt and Vd under biotic (Vd) and abiotic (salt) combined high stress conditions. It was also shown that Fm had a different effect on membrane injury index (MII) (%) values than Bc, which had a significant effect on leaf relative water content (LRWC) (%) values. In addition, Vd increased mycorrhizal dependence, and the highest AMF spore density under Vd and salt stress conditions was recorded in Fm and Fm+Bc+150 mM applications, respectively. Meanwhile, the highest root colonization rates were obtained in Fm and Fm + 100 mM applications at 92% and 64%, respectively. On the other hand, the lowest disease severity index values were found in Fm + Vd treatments with 11.06% and 9.68% rates, respectively. In addition, phenol and antioxidant values varied in applications with Vd, while Fm decreased CAT enzyme activation. This study, thus, supports the positive effect of AMF + biochar interaction used in sustainable agriculture on protection against soil pathogen (V. dahliae) and salt stress.
Silica nanoparticles induce apoptosis via the unfolded protein response in NRK-52E cells and rat kidney
Authors: Liu Naimeng , Li Meng , Pang Huan , Tiantian Tian , Li Xinyue , Jin Minghua , Wu Hao , Qian Chuyue , Sun Mindan 
Published: May 2023

Abstract
Silica nanoparticles (SiNPs) have multiple applications, particularly in the field of biomedical science. However, SiNPs can cause a multitude of diseases, including chronic kidney disease (CKD). The molecular mechanism of renal toxicity caused by SiNPs is complex and remains to be clarified. Therefore, we examined the role and mechanism of apoptosis via the unfolded protein response (UPR) induced by SiNPs. We utilized an in vitro model of NRK-52E cells and an in vivo rat model with SiNPs administered via tracheal drip. After the NRK-52E cells were exposed to SiNPs, cell viability decreased; the mitochondrial membrane potential, calcium content, reactive oxygen species, and apoptosis rate increased; and light microscopy revealed cell damage. Meanwhile, apoptosis, the UPR, and oxidative stress-related proteins were all increased in NRK-52E cells. Moreover, an increase in the concentration of SiNPs was positively correlated with renal damage, as detected by light microscopy and transmission electron microscopy. As the SiNP concentration increased, apoptosis, the UPR, and oxidative stress-related proteins increased and the activity of antioxidant enzymes decreased in rat kidney. We conclude that the UPR plays a key role in apoptosis induced by SiNPs in the kidney.
Tissue-specific transcriptome and metabolome analyses reveal candidate genes for lignan biosynthesis in the medicinal plant Schisandra sphenanthera
Authors: Sun Boshi , Wang Peng , Guan Meng , Jia Entong , Li Qian , Li Jun , Zhou Ziyun , Ma Pengda 
Published: May 2023

Abstract
Schisandra sphenanthera is an very important medicinal plant, and its main medicinal component is bioactive lignans, which has been developed over the years as an important cash crop in the central mountainous. The S. sphenanthera fruit has the characteristics of food and medicine homology, which is favored by the majority consumers, but the root, stem, and leaf are not fully used. Furthermore, the genetic characteristics of S. sphenanthera are rarely understood, thus hindering the study of functional genome and lignan biosynthesis. To better understand the lignan metabolic pathway, transcriptome and metabolome analysises were performed on four major tissues in S. sphenanthera. As a consequence, 167,972,229 transcripts and 91,215,760 unigenes with an average length of 752 bp were identified. Tissue-specific gene analysis revealed that the abundance of unique unigenes was highest in roots (9, 703), and lowest in leaves (189). Transcription factor analysis showed that MYB-, bHLH- and ERF-transcription factors, which played important roles in the regulation of secondary metabolism, showed rich expression patterns and may be involved in the regulation of the lignan metabolic processes. In the different tissues, lignans were preferentially enriched in fruits and roots by the genes expression profiles related to lignan metabolism and lignan compounds relative content. Furthermore, schisandrin B was found to be an important compound in S. sphenanthera. According to WGCNA analysis, PAL1, C4H-2, CAD1, CYB8, OMT27, OMT57, MYB8, bHLH3, and bHLH5 could be related to the accumulation of lignans in S. sphenanthera fruits, CCR5, SDH4, CYP8, CYP20 and ERF7 could be related to the accumulation of lignans in S. sphenanthera roots. In this study, the transcriptome sequencing and targeted metabolic analysis of lignins that will lay a foundation for further study of biosynthetic genes of lignin and other natural products in S. sphenanthera, and also provided a new idea for the rational utilization of different tissues of S. sphenanthera.
Investigating the Health Status of Transgender Individuals Referred to Forensic Medicine Center in 2021: A Comparative Cross-Sectional Study in Iran
Authors: Heshmatnia Fatemeh , Jafari Maryam , Sayadi Mehrab , Yadollahi Parvin , Azizi Marzieh , Karimi Mahdieh , Ghaemi Seyede Zahra , Rahmanian Fatemeh 
Published: May 2023

Abstract
Background & Aim
This study aimed to determine health status of Iranian transgender people who referred to Forensic Medicine Center in order to provide equal and accessible health services to transgender people.
Materials and methods
As part of this cross-sectional study, 200 transsexuals (106 transgender men/TM, 94 transgender women/TW) from Fars Province, Iran, completed a questionnaire including 24 items on socio-demography and health status in 2021. The data were analyzed using SPSS version 16, and also Chi-square test and Fisher's exact test were used for the data analysis. A p-value < 0.05 was considered statistically significant.
Results
The gender identity age distribution was significantly different between TW and TM (P = 0.004). Women were significantly more likely to engage in unprotected intercourse than men (p = 0.001), but perceived financial, medical, and social barriers were not significantly different between the two groups (P > 0.05). However, women reported more family obstacles than men (P = 0.006).
Conclusion
Both women and men have experienced equal financial, medical, and social challenges in the health system, but women face more constraints in their family lives.
Mechanistic inhibition of human papillomavirus-associated cervical cancer and DNA polymerase theta receptor by natural Apigenin derivatives: An extensive drug design and computational approach
Authors: Akash Shopnil , Bayıl Imren , Hossain Md. Saddam , Islam Md. Rezaul , Hosen Md. Eram , Mekonnen Amare Bitew , Nafidi Hiba-Allah , Jardan Yousef A. Bin , Bourhia Mohammed 
Published: May 2023

Abstract

The present study deals with the advancedin-silicoanalyses of several Apigenin derivatives to explorehuman papillomavirus-associated cervicalcancerand DNA polymerase theta inhibitor propertiesby molecular docking, molecular dynamics, QSAR, drug-likeness, PCA, a dynamic cross-correlation matrix and quantum calculation properties. Initially, the literature study revealed that Apigenin has potent antimicrobial and anticancer properties. So, their potential derivatives are picked up to determinehuman papillomavirus-associated cervicalcancerand DNA polymerase theta inhibitor properties. The findings were streamlined by exposing the in silico molecular docking, highlighting that all the Apigenin derivatives exhibit prospective interactions of energy binding with targeted proteins. The Apigenin 4'-O-Rhamnoside and Apigenin-4'-Alpha-L-Rhamnoside were more potent against HPV45 oncoprotein E7 (PDB ID 2EWL), where the Apigenin and Apigenin 5-O-Beta-D-Glucopyranoside showed significant binding energy against L1 protein of human. Similarly, the binding affinity range is achieved from -7.5 kcal/mol to -8.8 kcal/mol against DNA polymerase theta, representing that Apigenin derivatives should inhibit the DNA polymerase theta (PDB ID 8E23). Then, this finding was confirmed by molecular dynamic simulation at 100ns and analysis of different parameters such as RMSD, RMSF, SASA, H-bond, and RoG profiles and it is found that our selected compounds were found to be potentially stable. When stability testing is passed, the ADMET, pharmacokinetics, and drug-likeness properties are screened and fulfilled by all the criteria. Moreover, QSAR, PCA, Dynamic cross-correlation matrix, and quantum calculation were also conducted and the outcome was satisfied. As this study was carried out usingin silicocomputational approaches and obtained outstanding results. So, to validate these results, further wet-lab experiments might be carried out underin vivoandin vitroconditions.