bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ecological network structure in response to community assembly processes over evolutionary time
Authors: Graham Natalie R. , Krehenwinkel Henrik , Lim Jun Ying , Staniczenko Phillip , Callaghan Jackson , Andersen Jeremy C. , Gruner Daniel S. , Gillespie Rosemary G. 
Published: February 2023

AbstractThe dynamic structure of ecological communities results from interactions among taxa that change with shifts in species composition in space and time. However, our ability to study the interplay of ecological and evolutionary processes on community assembly remains relatively unexplored due to the difficulty of measuring community structure over long temporal scales. Here, we made use of a geological chronosequence across the Hawaiian Islands, representing 50 years to 4.15 million years of ecosystem development, to sample 11 communities of arthropods and their associated plant taxa using semiquantitative DNA metabarcoding. We then examined how ecological communities changed with community age by calculating quantitative network statistics for bipartite networks of arthropod–plant associations. The average number of interactions per species (linkage density), ratio of plant to arthropod species (vulnerability) and uniformity of energy flow (interaction evenness) increased significantly in concert with community age. The index of specialization has a curvilinear relationship with community age. Our analyses suggest that younger communities are characterized by fewer but stronger interactions, while biotic associations become more even and diverse as communities mature. These shifts in structure became especially prominent on East Maui (~0.5 million years old) and older volcanos, after enough time had elapsed for adaptation and specialization to act on populations in situ. Such natural progression of specialization during community assembly is probably impeded by the rapid infiltration of non‐native species, with special risk to younger or more recently disturbed communities that are composed of fewer specialized relationships.
Optical characterisation of two novel myopia control spectacle lenses
Authors: Gantes‐Nuñez Javier , Jaskulski Matt , López‐Gil Norberto , Kollbaum Pete S. 
Published: February 2023

AbstractPurposeTo quantify the amount of myopic defocus, contrast modulation and other optical characteristics of two novel spectacle lenses (MiYOSMART by Hoya and Stellest by Essilor) with the inclusion of lenslets in their designs were investigated computationally and experimentally. This paper examined the hypothesis that despite the non‐coaxial nature of the optics, image degradation will exist due to the fragmented nature of the base optic when imaging through the lens regions populated by lenslets.MethodsOptical power was evaluated by computing wavefront vergence and curvature from wavefront slope measured with the Optocraft aberrometer within 1.0 and 6.0 mm apertures across MiYOSMART hexagons and Stellest rings. Point‐spread functions (PSFs) were computed using physical (wave) optics and geometrical ray optics principles, and compared with experimental measurements using a 4f optical system. Simulated retinal images and modulation transfer functions (MTFs) were computed from PSF‐derived optical transfer functions (OTFs).ResultsMean lenslet power in MiYOSMART was +3.95 ± 0.10 D through the hexagons and +6.00 ± 0.15 D in Stellest in rings 1–5 and decreased by 0.42 D/ring reaching 3.50 D in the final one. Stellest lenslets included up to −0.015 microns of primary spherical aberration. PSFs and retinal images revealed simultaneous contributions of the base optic and lenslets. MTFs showed a decrease in contrast at low (1–10 c/deg) spatial frequencies (SFs) comparable to 0.25 D of defocus, and retention of diminished levels of contrast at higher SFs.ConclusionsVarying sagittal power and consistent curvature power across the lenslets is an identifying signature of the novel non‐coaxial lens design included in both spectacle lenses. Lenslet array structure itself plays a significant role in determining image characteristics. For both lenses, the blur created by the fragmented base optic contributes to the image quality. The reduced MTFs over a wide range of spatial frequencies result in lowered image contrast.
Specific virulence patterns in Tunisian Zymoseptoria tritici strains isolated from bread and durum wheat
Authors: Ben M’Barek Sarrah , Laribi Marwa , Abdedayem Wided , Fakhfakh Moez , Yahyaoui Amor Hassine 
Published: February 2023

AbstractSeptoria tritici blotch caused by Zymoseptoria tritici is a serious threat to wheat in Tunisia, causing yield losses of up to 50%. Epidemics occur regularly, mainly on durum wheat (DW) but also to a lesser extent on bread wheat (BW), that are mainly cropped in the north‐western and north‐eastern regions, respectively. Isolates of Z. tritici exhibit both cultivar specificity and host species specificity. In this study, we investigated the extent of host specialization of Z. tritici in the Tunisian context. Cross‐inoculations of different strains derived from either BW or DW were performed at the seedling stage on a panel of the most cultivated BW and DW varieties in Tunisia using the percentage of pycnidia coverage and necrotic leaf area as disease parameters. Results showed that the effect of strains, the strain‐derived species, the wheat species and of their interactions were highly significant based on pycnidia coverage. This indicates the presence of significant virulence variability among Z. tritici strains on the tested wheat genotypes, with some strains being more virulent than others. It also suggests cultivar and host species specificity, as not only genotypes of the strains interacted differently with the genotypes of the host, but also the origin of the strain, whether BW or DW, influenced the disease outcome. Hence, future research based on comparative genomics coupled with transcriptomics of these strains and their progenies could determine genes whose expression control this host specificity or is influenced by host or strain genotype, or alternatively by life cycle stage.
Radial artery superficialization for hemodialysis patients with severe heart failure: A case report
Authors: Zhang Xi , Tu Bo , Wan Ziming 
Published: February 2023

AbstractArteriovenous fistula (AVF) and/or arteriovenous graft (AVG) burdens cardiac load. It is a challenge to create a vascular access (VA) on a patient who suffers severe heart failure and also combines with contraindications of central vein catheter implantation. Superficialized radial artery (RA) could be an available VA as an outflow for continuous hemodialysis. A 57‐year‐old male had a left arm distal radiocephalic fistula for hemodialysis for 10 months. The patient showed gradual aggravation of heart failure. RA superficialization was performed under anesthesia protocols and surgery steps. Fourteen days after the surgery, the superficialized artery supplied the hemodialysis as an outflow without any uncomfortable and complications. The echocardiographies present the changes of the heart structures and functions during the whole period from the beginning of AVF formation to the 18‐month follow‐up RA superficialization. RA superficialization technique should be an alternative VA for continuous hemodialysis under suitable conditions.
The Evental Conception of Love
Authors: Shmugliakov Pioter 
Published: February 2023

AbstractThis article proposes a critical presentation and development of Alain Badiou's theory of romantic love, at the center of which is an understanding of the phenomenon in terms of a truth‐generating event. I discuss this notion against the more familiar ontological modes of theorizing love: as the subject's intentional attitude and as an activity of internal value. Arguing that the evental conception of love poses a preferable alternative to the former mode, my analysis focuses on its complementary relations with the latter, of which I take Stanley Cavell's theory of marriage as a representative. My further argument is concerned with the place of sexuality in the evental conception of love, compensating for what I argue to be the shortcomings of Badiou's treatment of the topic by turning to Roger Scruton's account of the immanent significance of the sexual.
Informal social capital building in local employment services: Its role in the labour market integration of disadvantaged young people
Authors: Rodríguez‐Soler Joan , Verd Joan Miquel 
Published: February 2023

AbstractThis article focuses on the role that local employment services (LES) play in informal social capital building for young people with a disadvantaged social background. The personal networks of these young people usually embed few useful resources for labour market integration, and LES actions may be an opportunity to informally build a network of contacts with better resources. To explore this possibility, biographical interviews were conducted with 24 young adults who used LES actions, and their employment trajectories were analysed. The findings highlight that these actions—especially those consisting of training or internships—provide social capital that is useful for labour market (re)integration. Linking social capital is the type of social capital that is most useful for job seeking, and it is especially effective in non‐metropolitan rural areas.
Exon shuffling potentiates a diverse repertoire of brown algal NB‐ARC‐TPR candidate immune receptor proteins via alternative splicing
Authors: Teng Linhong , Liang Miao , Wang Chenghui , Li Yan , Urbach Jonathan M. , Kobe Bostjan , Xing Qikun , Han Wentao , Ye Naihao 
Published: February 2023

SUMMARYLike other organisms, brown algae are subject to diseases caused by bacteria, fungi, and viruses. Brown algal immunity mechanisms are not well characterized; however, there is evidence suggesting that pathogen receptors exist in brown algae. One key protein family likely associated with brown algal innate immunity possesses an NB‐ARC domain analogous to innate immune proteins in plants and animals. In this study, we conducted an extensive survey of NB‐ARC genes in brown algae and obtained insights into the domain organization and evolutionary history of the encoded proteins. Our data show that brown algae possess an ancient NB‐ARC‐tetratricopeptide repeat (NB‐TPR) domain architecture. We identified an N‐terminal effector domain, the four‐helix bundle, which was not previously found associated with NB‐ARC domains. The phylogenetic tree including NB‐ARC domains from all kingdoms of life suggests the three clades of brown algal NB‐TPRs are likely monophyletic, whereas their TPRs seem to have distinct origins. One group of TPRs exhibit intense exon shuffling, with various alternative splicing and diversifying selection acting on them, suggesting exon shuffling is an important mechanism for evolving ligand‐binding specificities. The reconciliation of gene duplication and loss events of the NB‐ARC genes reveals that more independent gene gains than losses have occurred during brown algal evolution, and that tandem duplication has played a major role in the expansion of NB‐ARC genes. Our results substantially enhance our understanding of the evolutionary history and exon shuffling mechanisms of the candidate innate immune repertoire of brown algae.
Mdm‐miR160–MdARF17–MdWRKY33 module mediates freezing tolerance in apple
Authors: Shen Xiaoxia , Ping Yikun , Bao Chana , Liu Chen , Tahir Muhammad Mobeen , Li Xuewei , Song Yi , Xu Weirong , Ma Fengwang , Guan Qingmei 
Published: February 2023

SUMMARYApple (Malus domestica) trees are vulnerable to freezing temperatures. Cold resistance in woody perennial plants can be improved through biotechnological approaches. However, genetic engineering requires a thorough understanding of the molecular mechanisms of the tree's response to cold. In this study, we demonstrated that the Mdm‐miR160–MdARF17–MdWRKY33 module is crucial for apple freezing tolerance. Mdm‐miR160 plays a negative role in apple freezing tolerance, whereas MdARF17, one of the targets of Mdm‐miR160, is a positive regulator of apple freezing tolerance. RNA sequencing analysis revealed that in apple, MdARF17 mediates the cold response by influencing the expression of cold‐responsive genes. EMSA and ChIP‐qPCR assays demonstrated that MdARF17 can bind to the promoter of MdWRKY33 and promotes its expression. Overexpression of MdWRKY33 enhanced the cold tolerance of the apple calli. In addition, we found that the Mdm‐miR160–MdARF17–MdWRKY33 module regulates cold tolerance in apple by regulating reactive oxygen species (ROS) scavenging, as revealed by (i) increased H2O2 levels and decreased peroxidase (POD) and catalase (CAT) activities in Mdm‐miR160e OE plants and MdARF17 RNAi plants and (ii) decreased H2O2 levels and increased POD and CAT activities in MdmARF17 OE plants and MdWRKY33 OE calli. Taken together, our study uncovered the molecular roles of the Mdm‐miR160–MdARF17–MdWRKY33 module in freezing tolerance in apple, thus providing support for breeding of cold‐tolerant apple cultivars.
PbABCG1 and PbABCG2 transporters are required for the emission of floral monoterpenes in Phalaenopsis bellina
Authors: Chang Ya‐Lan , Huang Li‐Min , Kuo Xuan‐Zhou , Chen You‐Yi , Lin Shao‐Ting , Jeng Mei‐Fen , Yeh Hsin‐Hung , Tsai Wen‐Chieh , Chen Hong‐Hwa 
Published: February 2023

SUMMARYTerrestrial plants emit volatiles into the atmosphere to attract both pollinators and the enemies of herbivores, for defense. Phalaenopsis bellina is a scented orchid species in which the main scent components are monoterpenes, including linalool and geraniol, and their derivatives. Here, we investigated whether ABC transporters are involved in floral scent emission. We carried out whole‐genome identification of ABC transporter‐related genes using four floral transcriptomics libraries of P. bellina. We identified 86 ABC subfamily G genes related to terpenoid transport. After comparing the gene expression patterns of P. bellina with that of Phalaenopsis aphrodite subsp. formosana, a scentless species, followed by gene‐to‐gene correlation analysis, PbABCG1 and PbABCG2 were selected. The temporal expression of both PbABCG1 and PbABCG2 was highly correlated with that of the key enzyme PbGDPS and the major transcription factor PbbHLH4 in monoterpene biosynthesis, with optimal expression on day 5 post‐anthesis. Spatial gene expression analysis showed that PbABCG1 was highly expressed in sepals, whereas PbABCG2 was expressed in the lip. Subcellular localization with a GFP fusion protein revealed that both PbABCG1 and PbABCG2 are cytoplasmic membrane proteins. Co‐downregulation of PbABCG1 and PbABCG2 using both double‐strand RNA interference and tobacco rattle virus‐based gene silencing led to a significant decrease in monoterpene emission, accompanied by an increase in the internal monoterpene pools. Furthermore, ectopic expression of PbABCG1 and PbABCG2 in an ABC16− mutant yeast strain rescued its tolerance to geraniol. Altogether, our results indicate that PbABCG1 and PbABCG2 play substantial roles in monoterpene transport/emission in P. bellina floral scent.
A novel high‐prevalence antigen in the Lutheran system, LUGA (LU24), and an updated, full‐length 3D BCAM model
Authors: Floch Aline , Lomas‐Francis Christine , Vege Sunitha , Brennan Steven , Shakarian Gayane , de Brevern Alexandre G. , Westhoff Connie M. 
Published: February 2023

AbstractBackgroundThe basal cell adhesion molecule (BCAM) carries the antigens of the Lutheran (LU, ISBT005) system. We report a novel Lutheran antigen and propose an updated, full‐length 3D model of BCAM.Study Design and MethodsRed blood cell testing, antibody identification, and BCAM genomic DNA sequencing were done by standard methods. Multi‐template homology modeling of BCAM used structural templates selected for coverage, highest sequence identity, and protein domain family. All variants causing the loss or gain of a Lutheran antigen were analyzed for residue accessibility and intraprotein interactions.ResultsAn antibody to a high‐prevalence antigen in the plasma of a pregnant woman was determined to be directed at a novel Lutheran antigen. Sequencing of BCAM found three homozygous changes: c.212G > A (p.Arg71His) and two silent, c.711C > T and c.714C > T. The model was built from the first two immunoglobulin crystallized domains of BCAM (D1, D2), three other templates (for D3, D4 and D5 with a higher sequence identity with the target than those used for the model proposed by Burton and Brady in 2008, and for the transmembrane region) and RaptorX (for the intracellular domain). All residues associated with a Lutheran antigen were found to be exposed in wild‐type or variant proteins, except p.447 associated with loss of Lu13 expression.ConclusionThe c.212G > A change results in the loss of LUGA (LU24) antigen. Whole genome sequencing continues to reveal polymorphisms with uncertain immunogenicity. This model and demonstration that nearly all residues associated with the expression of a Lutheran antigen are exposed will help evaluate the significance of new polymorphisms.
Contrast‐enhanced ultrasound repeatability for the measurement of skeletal muscle microvascular blood flow
Authors: Jones Eleanor J. , Atherton Philip J. , Piasecki Mathew , Phillips Bethan E. 
Published: February 2023

AbstractContrast‐enhanced ultrasound (CEUS) can be used to directly assess skeletal muscle perfusion. However, its repeatability over time has not yet been validated and therefore its use in longitudinal measures (i.e., exploring the impact of a chronic intervention or disease progression) is limited. This study aimed to determine the repeatability of CEUS for the measurement of skeletal muscle microvascular blood flow (MBF) at baseline and in response to exercise, across independent assessment sessions. Ten healthy volunteers (five female; 30 ± 6 years) had CEUS of the right vastus lateralis recorded in two separate sessions, 14 days apart. Measurements were taken at baseline, during an isometric leg extension and during recovery. Acoustic intensity data from a region of interest were plotted as a replenishment curve to obtain blood volume (A) and flow velocity (β) values from a one‐phase association non‐linear regression of mean tissue echogenicity. Linear regression and Bland–Altman analyses of A and β values were performed, with significance assumed as P < 0.05. Strong positive correlations were observed across sessions for all A and β values (both P < 0.0001). Bland–Altman analysis showed a bias (SD) of −0.013 ± 1.24 for A and −0.014 ± 0.31 for β. A bias of 0.201 ± 0.770 at baseline, 0.527 ± 1.29 during contraction and −0.203 ± 1.29 at recovery was observed for A, and −0.0328 ± 0.0853 (baseline), −0.0446 ± 0.206 (contraction) and 0.0382 ± 0.233 (recovery) for β. A strong agreement between CEUS MBF measures across independent sessions suggests it to be a repeatable method for assessing skeletal muscle perfusion over time, and therefore facilitates wider use in longitudinal studies.
A Novel Ladder-Shaped Bridge Finned Tube for Convective Heat Transfer Enhancement
Authors: Wan Zhenping , Yang Yujie , Wang Xiaowu , Tao Sulian , Chen Hanping 
Published: February 2023

Abstract
In order to improve the convective heat transfer efficiency of a shell-and-tube heat exchanger, a novel ladder-shaped bridge finned tube (LBFT) is presented. The LBFT possesses outer low helical integral fins, two layers of staggered transverse bridge, upper passage, middle passage, and bottom passage. The convective heat transfer performance of the LBFT is studied and experimental results show that the Nusselt numbers outside the tube and the overall heat transfer coefficients of the LBFT are significantly greater than those of the smooth tube. The bridges, bridge roots, and pores formed on the outer fins contribute to the larger heat transfer coefficient. Both the Nusselt number and the overall heat transfer coefficient decrease, while the friction resistance coefficient increases with outer helical fin pitch increasing and bridge width increasing. As the Reynolds number increases, the comprehensive performance evaluation criterion (PEC) decreases at first and then increases. The maximum PEC occurs at the Re number of 2300 and is up to 1.34.
Critical Heat Flux Condition and Post-Critical Heat Flux Heat Transfer of Carbon Dioxide at High Reduced Pressures in a Microchannel
Authors: Parahovnik Anatoly , White Esther , Peles Yoav 
Published: February 2023

Abstract
Flow boiling heat transfer around the critical heat flux (CHF) condition at high reduced pressures of carbon dioxide in a 296-μm hydraulic diameter microchannel was experimentally studied. The CHF conditions for developing flow and fully developed flow were measured and compared to established correlations. The post-CHF heat transfer coefficient was obtained for l/d of 3.2, 7.4, and 11.6 for inlet Reynolds numbers, based on the homogeneous two-phase flow model, ranging from 6622 to 32,248. The critical heat flux conditions seemed to peak around a reduced pressure of about 0.5 and gradually decreased with reduced pressure. However, the typical rapid increase in the surface temperature following the CHF condition decreased with increasing pressure, and the post-CHF heat transfer coefficient was appreciably high (up to about 50 kW/m2K) at high reduced pressures. The enhancement in the heat transfer coefficient and CHF condition near the inlet were quantified. The experimental results were compared to established CHF correlations and heat transfer coefficient correlations with some limited success. Thus, the Katto CHF correlation (Katto and Ohno, 1984, “An Improved Version of the Generalized Correlation of Critical Heat Flux for the Forced Convective Boiling in Uniformly Heated Vertical Tubes,” Int. J. Heat Mass Transfer, 27(9), pp. 1641–1648) and the Bishop correlation (Bishop et al., 1964, “Forced-Convection Heat Transfer to Water at Near-Critical Temperatures and Supercritical Pressures,” Westinghouse Electric Corp, Atomic Power Division, Pittsburgh, PA.) for the post-CHF heat transfer coefficient were adjusted to better predict the experimental results. Additionally, an enhancement factor was derived to predict the increase in the heat transfer coefficient in the developing region.
Development of a Noninvasive Device to Predict Postoperative Ileus Using Acoustic Biomarkers
Authors: Hrabe Jennifer E. , Gribovskaja-Rupp Irena , Guyton Kristi , Kapadia Muneera R. , Cromwell John W. 
Published: February 2023

AbstractFor patients undergoing gastrointestinal surgery, postoperative ileus (POI) is difficult to predict and occurs at rates up to 30%. We hypothesized that discrete gastrointestinal acoustic biomarkers correlating to POI development may be present in early postoperative hours. To test this hypothesis, we conducted a two-phase investigation to record and analyze intestinal sounds, then to prospectively test the feasibility of predicting POI using a noninvasive device trained with an algorithm developed from the Phase 1 results. In Phase 1, a library of intestinal sounds from normal volunteer subjects and patients undergoing intestinal resection surgery were recorded. An acoustic biomarker most correlated with POI was identified and used to develop a predictive algorithm. In Phase 2, an autonomous, wearable device possessing listening and signal processing capability was developed and prospectively tested for prediction of POI in patients undergoing bowel resection surgery. In Phase 1, 30 patients undergoing intestinal resection surgery were studied, 9 of whom developed POI, from which an acoustic biomarker was identified and analyzed. In Phase 2, prospective prediction of POI in 75 enrolled subjects undergoing bowel resection surgery was assessed. POI was correctly predicted in 12 of 14 subjects who developed POI; prediction that POI would not occur was correct in 39 of 51 subjects. Sensitivity and specificity were 85.7% and 63.9%, respectively. Negative predictive value and accuracy were 95.1%, and 68%, respectively. Our study demonstrates feasibility of POI prediction based on gastrointestinal sounds using a noninvasive device. This device may help risk stratify patients likely to develop POI.