bluuebunny/crossref_metadata_2025_split huggingface dataset:
Orientin suppresses osteoclastogenesis and ameliorates ovariectomy‐induced osteoporosis via suppressing ROS production
Authors: Zheng Yan , Wang Xing , Pan Ya‐Jing , Shi Xiao‐Feng , Yang Lei , Lou Yong‐Liang 
Published: June 2023

AbstractThe aberrant differentiation of osteoclasts is a key feature of the pathogenesis of osteoporosis, which has a devastating impact on human health. While the effects of Orientin (Ori) on osteoporosis, particularly on RANKL‐stimulated osteoclast production and activation, remain still unclear, Ori has been found to display several biological activities, including antioxidant and anti‐inflammatory. In this work, we investigated the possible pathways through which Ori suppressed RANKL‐induced osteoclast development and showed for the first time that it does so. The macrophages from the bone marrow (BMMs) were cultivated and then treated with Ori after being stimulated with RANKL. Then, TRAP‐positive multinucleated cells were counted, and F‐actin ring analysis was used to assess Ori's impact on mature osteoclast development. In addition, dihydroethidium (DHE) staining was used to evaluate the impact of Ori on RANKL‐induced reactive oxygen species (ROS). In addition, we performed western blotting and quantitative RT‐PCR analysis to investigate probable causes of these downregulation effects. We discovered that Ori inhibits the creation of osteoclasts, the gene and protein expressions unique to osteoclasts, and the ROS production. By activating Nrf2 and other ROS‐scavenging enzymes, Ori reduces intracellular ROS levels. The expression of the main transcription factor of osteoclast development, c‐Fos, was downregulated together with NFATc1, CTSK, and NFATc2, thanks to Ori's inhibition of RANKL‐induced NF‐κB. Consistent with its in vitro antiosteoclastogenic action, Ori therapy in the ovariectomized (OVX) rat model was also able to restore bone mass and improve microarchitecture in the distal femurs. Together, our results demonstrate that Ori is a flavonoid molecule with therapeutic promise for bone illnesses associated with osteoclasts, such as osteoporosis.
Differential effects of expectancy on memory formation in young and older adults
Authors: Steiger Tineke K. , Yousuf Mushfa , Bunzeck Nico 
Published: June 2023

AbstractNovelty can promote subsequent long‐term memory via the mesolimbic system, including the medial temporal lobe and midbrain structures. Importantly, these and other brain regions typically degenerate during healthy aging, which suggests a reduced impact of novelty on learning. However, evidence in favor of such a hypothesis is scarce. Thus, we used functional MRI in combination with an established paradigm in healthy young (19–32 years, n = 30) and older (51–81 years, n = 32) humans. During encoding, colored cues predicted the subsequent presentation of either a novel or previously familiarized image (75% cue validity), and approximately 24 h later, recognition memory for novel images was tested. Behaviorally, expected novel images, as compared to unexpected novel images, were better recognized in young and, to a lesser degree, older subjects. At the neural level, familiar cues activated memory related areas, especially the medial temporal lobe, whereas novelty cues activated the angular gyrus and inferior parietal lobe, which may reflect enhanced attentional processing. During outcome processing, expected novel images activated the medial temporal lobe, angular gyrus and inferior parietal lobe. Importantly, a similar activation pattern was observed for subsequently recognized novel items, which helps to explain the behavioral effect of novelty on long‐term memory. Finally, age‐effects were pronounced for successfully recognized novel images with relatively stronger activations in attention‐related brain regions in older adults; younger adults, on the other hand, showed stronger hippocampal activation. Together, expectancy promotes memory formation for novel items via neural activity in medial temporal lobe structures and this effect appears to be reduced with age.
COVID‐19 pandemic and socio‐environmental inequality: A narrative review
Authors: Kalankesh Laleh R. , Rezaei Zahed , Mohammadpour Ali , Taghavi Mahmoud 
Published: June 2023

AbstractBackground and AimsThe COVID‐19 pandemic has provided preliminary evidence of the existence of health, social, and environmental inequalities. This inequality encompasses inadequate access to safe water, clean air, and wastewater management, as well as limited socioeconomic and educational opportunities. These issues have not received sufficient attention during the pandemic. The purpose of this narrative review is to provide a comprehensive summary and analysis of the existing literature on a specific topic, ultimately leading to a conclusion based on the evidence presented.MethodsThe search methodology for this study involved conducting comprehensive searches of scientific databases, including PubMed, ScienceDirect, LILACS, and Google Scholar, from 2019 to 2023. The study focused on a specific theme and its relevant aspects related to global environmental health and society. Keywords such as COVID‐19, inequities, and environmental health were used for searching. Additionally, the Boolean operator “AND” was used to combine these descriptors.ResultsUnequal exposure to air pollution has been reported in Africa, as well as in large parts of Asia and Latin America, according to the data that has been obtained. The pandemic has also resulted in a surge in healthcare waste generation, exacerbating the environmental impact of solid waste. Furthermore, there is evidence indicating significant disparities in the severe lack of access to sanitation services between developing nations and low‐income regions. The issues related to water availability, accessibility, and quality are subject to debate. It has been reported that SARS‐CoV‐2 is present not only in untreated/raw water, but also in water bodies that act as reservoirs. Moreover, insufficient education, poverty, and low household income have been identified as the most significant risk factors for COVID‐19 infection and mortality.ConclusionIt is evident that addressing socio‐environmental inequality and striving to narrow the gap by prioritizing vulnerable populations are imperative.
The role of IL‐6/JAK/STAT signal in female infertility caused by hydrosalpinx
Authors: Deng Jia , Huang Yufu , Wang Luying , Sun Xin 
Published: June 2023

AbstractIntroductionTo explore the role of IL‐6/JAK/STAT signaling in tubal infertility.MethodsThe fimbriae tissues of 14 patients with a history of infertility and hydrosalpinx and 14 patients with no history of infertility and no fallopian tube disease were collected. The tissues were then divided into hydrosalpinx group and control group followed by analysis of the protein expression of key factors in the IL‐6/JAK/STAT signaling by immunohistochemistry and Western blot.ResultsImmunohistochemical staining showed significantly higher level of IL‐6, JAK1, p‐JAK1, JAK2, p‐JAK2, STAT1, p‐STAT1, STAT3, and p‐STAT3 in hydrosalpinx group than those in control group with IL‐6 being mainly located in the cytoplasm and p‐JAK2, STAT1, p‐STAT1, STAT3, and p‐STAT3 in the cytoplasm and nucleus. JAK1 and p‐JAK1 was mainly located in the cytoplasm and JAK2 is in the cytoplasm and nucleus without difference of their expression between two groups. Consistently, hydrosalpinx group presented significantly higher protein levels of IL‐6, JAK1, p‐JAK1, JAK2, p‐JAK2, STAT1, p‐STAT1, STAT3, and p‐STAT3 than control group without difference of JAK1, p‐JAK1, JAK2 level.ConclusionThe activation of IL‐6/JAK2/STAT1 and STAT3 signaling pathways are found in the hydrosalpinx in infertile patients, indicating that they might be involved in the pathogenesis of hydrosalpinx.
The clinical characteristics of patients with asthma exposed to different environmental risk factors: A cross‐sectional study
Authors: Luo Laiheng 
Published: June 2023

AbstractBackgroundSmoking, biomass, and occupational exposure are the main environmental risk factors for asthma. The purpose of this study was to analyze the clinical characteristics of exposure to these risk factors in patients with asthma.MethodsThis cross‐sectional study enrolled patients with asthma from an outpatient department according to the Global Initiative for Asthma. Demographics, forced expiratory volume in 1 s (FEV1), FEV1%pred, FEV1/forced vital capacity (FVC), laboratory tests, asthma control test (ACT), asthma control questionnaire (ACQ) scores, and the inhaled corticosteroid (ICS) dose were recorded. A generalized linear mixed model was used to adjust for potential confounders.ResultsA total of 492 patients with asthma were included in this study. Of these patients, 13.0% were current smokers, 9.6% were former smokers, and 77.4% were never smokers. Compared with never smokers, the current and former smokers had a longer duration of asthma; lower ACT scores, FEV1, FEV1%pred, and FEV1/FVC; and higher ACQ scores, IgE, FeNO, blood eosinophils, and ICS dose (p < .05). In addition, the patients exposed to biomass alone were older; had higher exacerbation in the past year; a longer duration of asthma; and lower FEV1, FEV1%pred, FEV1/FVC, IgE, and FeNO compared with smoking or occupational exposure alone. Compared with smoking exposure alone, patients with occupational exposure alone had a longer duration of asthma and lower FEV1, FEV1%pred, FVC, IgE, FeNO, and ICS dose (p < .05).ConclusionsThere are significant differences in the clinical characteristics of patients with asthma depending on the smoking status. In addition, significant differences also observed among smoking, biomass, and occupational exposure.
Intraoperative temporary internal iliac arterial occlusion (Polat's technique) for severe placenta accreta spectrum: A description of the technique and outcomes in 61 patients
Authors: Daggez Mine , Aslanca Tufan , Dursun Polat 
Published: June 2023

AbstractObjectiveTo report the results of prophylactic use of intraoperative temporary internal iliac arterial occlusion by Bulldog clamps in patients clinically diagnosed with abnormally invasive placenta.MethodsThis retrospective study included 61 patients diagnosed with FIGO grade 3 abnormally invasive placenta between January 2018 and March 2022. After transfundal incision and fetal delivery, bilateral temporary internal iliac arterial occlusion by Bulldog clamps was performed in all patients. The grades 3b and 3c group underwent cesarean hysterectomy whereas selected cases of grade 3a abnormally invasive placenta underwent fertility‐preserving procedures. Preoperative and postoperative findings were compared.ResultsCesarean hysterectomy was performed in 50 (82%) patients and cesarean plus conservative procedures were performed in 11 (18%) patients. Intraoperative blood replacement was not performed in 83.6% of all patients. Mean blood loss was 1.37 ± 0.53 L (range 0.5–2.5) in all patients. Estimated blood loss was significantly higher in cesarean hysterectomy group. There was no statistically significant difference between two groups in terms of peroperative blood replacement, bladder, and ureteral injury.ConclusionProphylactic bilateral temporary internal iliac arterial occlusion by Bulldog clamps should be performed in cases of grade 3 abnormally invasive placenta. Fertility‐preserving steps may be undertaken safely in selected cases with this approach.
Agile MBSE: Doing the Same Thing We Have Always Done, but in an Agile Way with Models
Authors: Hause Matthew 
Published: June 2023

ABSTRACTAgile systems engineering is not new. Work has progressed on this for many years to the point that criteria has been established regarding best practice as well as a means of quantifying adherence. The future of systems engineering (FuSE) initiative is reexamining how agile systems engineering fits into the FuSE (Willette et al. 2021). As model‐based systems engineering (MBSE) is also a FuSE theme, it is only proper to look at how agile systems engineering and MBSE complement and enable each other. This article examines some of the aspects of MBSE–specifically the Systems Modeling Language® (SysML) – and show at how an agile approach to MBSE can help with the concepts of stakeholder engagement, continual integration, and dynamic learning and evolution. For reasons of space, the article will only provide minimal definitions and explanations of the basics of MBSE, agile, and SysML and as these are well known concepts.
An Agile Systems Engineering Process for Stakeholder Needs Identification and Solution Concept Design
Authors: Castro Lymari 
Published: June 2023

ABSTRACTThis paper presents a case study where an agile systems engineering process was used to identify stakeholder needs to design an improved cross‐organizational proposal development process during the proposal formulation phase of a program. The agile systems engineering process leveraged the incremental application of design thinking techniques to engage the stakeholders and identify the care‐abouts of an organization during proposal formulation in support of a change management effort. The goal of the change management effort was to design solutions that increased collaboration and engagement across the various internal and external stakeholders without changing the overarching corporate proposal development process. The identified solutions broke existing organizational silos and changed the dynamics of the organization impacting over 1,200 employees. The case study relates to the future of systems engineering (FuSE) concepts of stakeholder engagement and agility across organizational boundaries.
Applying Agility for Sustainable Security
Authors: Rosser Larri Ann 
Published: June 2023

ABSTRACTSystems engineering faces ongoing challenges due to the pace of change in technology and needs as well as the complexity, resilience, and adaptability demanded of solutions. System security needs and challenges are a prominent factor in the increasing demands placed on solutions and the systems engineers who design and develop them. The adoption of program level agile execution is one strategy for addressing these escalating challenges. In this article we describe how the broadly adopted technical processes from the ISO/IEEE/IEC 15288:2015 standard can be executed using agile methods to realize a large complex solution. In addition, we provide specific recommendations for executing these processes in a manner that enables systems to be sustainably secure – that is, to retain the desired level of security throughout the life cycle.
Physiological and biochemical responses of Carthamus tinctorius L. to zinc at vegetative stage
Authors: Smaoui Ameni , Mahmoudi Hela , Medimagh Sana , Taheri Ali , Zribi Fethia , Ouerghi Zeineb , Ben Salah Imene 
Published: June 2023

AbstractBackgroundEnvironment contamination with heavy metals is a major problem both to the environment and human health.AimsThe aim of this work is to evaluate the physiological, nutritional, and biochemical responses of safflower plants (Carthamus tinctorius L.) grown under different zinc concentrations at vegetative stage.MethodsThe effect of zinc on safflower plants was studied through the evaluation of growth parameters, colorimetric assay of phenolic compounds, and antioxidant enzymes activities.ResultsZinc inhibited shoot and root growth and resulted in leaf chlorosis at 75, 225, and 1000 µM zinc concentrations. At higher concentrations, Zn also reduced photosynthetic parameters except the internal CO2 concentration that was higher at these levels. Zinc reduced potassium content of the shoots at 75 µM of zinc. The presence of Zn at 1000 µM concentration reduced the iron content and led to the accumulation of the copper content in the shoots. In roots, iron and copper contents were reduced from 15 µM Zn. In the case of copper, this content was canceled out at 225 and 1000 µM of zinc. Lipid peroxidation (evaluated as thiobarbituric acid reactive substances content) increased with zinc treatment. Safflower plants generally responded to this oxidative stress by enhancing the antioxidant enzymes activities, namely, catalase, ascorbate peroxidase, and guaiacol peroxidase. There was no increase in superoxide dismutase activity in shoots and roots, except at the highest zinc concentration in the roots. Total soluble sugars (TSS) and starch contents increased, whereas proline content decreased in shoots. In roots, TSS was not altered, whereas starch was decreased, and proline content was increased. There were no changes in the protein content in both organs under different Zn treatments. There was a gradual increase in the total polyphenols in shoots with increasing zinc concentration; however, no changes were observed in the roots. Flavonoids decreased in roots at 225 and 1000 µM Zn. Total antioxidant activity increased in shoots but it decreased in roots with increasing zinc concentration. The inverse trend was observed for the DPPH and ABTS tests.ConclusionsOur results suggest that the decline in safflower plant growth under high zinc concentrations is related to altered photosynthetic activity and antioxidant response to oxidative stress.
Synthesis, Characterization, and Anticancer Potency of Branched Poly (p‐Hydroxy Styrene) Schiff‐Bases
Authors: Kenawy El‐Refaie , El‐Khalafy Sahar H. , Abosharaf Hamed A. , El‐nshar Esraa M. , Ghazy Ahmed R. , Azaam Mohamed M. 
Published: June 2023

AbstractA significant issue in cancer biology is finding anticancer therapies that effectively kill cancer cells. Through the use of several aldehydes, Schiff bases based on branched poly (p‐hydroxy styrene) are created. The branched polymer is first chloroacetylated, then aminated with 1,4‐phenylenediamine, and finally, aldehydes are reacted with the aminated polymer to produce the Schiff base compounds. Through the utilization of FTIR, TGA, XRD, NMR, and elemental analysis, all synthesized Schiff‐bases are identified and characterized. Further, the antineoplastic potential of all Schiff bases is evaluated against different cancer cell lines. The results gained from this study indicate that the Schiff base polymers have cytotoxic power against cancer cells depending on cancer cell type and this antiproliferation potency is dose‐concentration dependent. Importantly, the prepared S1 Schiff‐base polymer shows potent cytotoxicity and is able to trigger the apoptosis and reactive oxygen species (ROS) in MCF‐7 cells. Further, it downregulates VEGFR protein expression. The Schiff base polymers would have extensive applications in the biological disciplines.
How Phosphorous Flame Retardant Additives Affect Benzoxazine‐Based Monomer and Polymer Properties
Authors: Haubold Thorben S. , Wolter Nick , Sandinge Anna , Blomqvist Per , Mayer Bernd , Koschek Katharina 
Published: June 2023

AbstractThe phosphorous‐based flame retardant additives poly(m‐phenylene methylphosphonate) (PMP) and resorcinol bis(diphenyl phosphate) (RDP) are reacted with bisphenol F and aniline–based benzoxazine (BF‐a). DSC, rheological analysis, FT‐IR, and soxhlet extraction reveal the covalent incorporation of both FR additives—initiating phenols in PMP structure as well as free phenols generated via transesterification reaction in the case of RDP. In contrast to PMP, RDP elongates the processing window but decreases the thermo–mechanical properties. Both additives increase the resistance in reactions against small flames with solely a phosphorous loading of 0.3 wt%, resulting in a V‐0 rating and an improvement in the OI value by up to 2% for RDP and 4% for PMP. Both FRs reduce the heat release rate but increase the smoke production and the smoke toxicity in the case of RDP.
A Transparent Additive‐Free Fibrous Facial Mask for Skin Moisturizing
Authors: Fan Xiaoyan , Bai Xue , Tan Langqun , Fang Cheng , Shen Xinlei , Xu Haijuan , Wang Yuanduo , Ma Yulong , Li Yuewen , Song Xinyi , Hu Jiang , Lu Yuhan , Wei Xinfang , Zhang Junxin , Li Fengguang , Zhu Zhenghui , Duan Shuxia , Zhou Yongheng , Wu Haibo , Liu Wanjun 
Published: June 2023

AbstractMost sheet facial masks for skincare are made of nonwovens and loaded with liquid active ingredients, which are usually opaque and require additives for long‐term preservation. Herein, a Transparent Additive‐Free Fibrous (TAFF) facial mask is reported for skin moisturizing. The TAFF facial mask consists of a bilayer fibrous membrane. The inner layer is fabricated by electrospinning functional components of gelatin (GE) and hyaluronic acid (HA) into a solid fibrous membrane to get rid of additives, the outer layer is an ultrathin PA6 fibrous membrane that is highly transparent, especially after absorbing water. The results indicate that the GE‐HA membrane can quickly absorb water and become a transparent hydrogel film. By employing the hydrophobic PA6 membrane as the outer layer, directional water transport is achieved, which enables TAFF facial mask with excellent skin moisturizing effect. The skin moisture content is up to 84% ± 7% after placing the TAFF facial mask on the skin for 10 min. In addition, the relative transparency of the TAFF facial mask on the skin reaches 97.0% ± 1.9% when ultrathin PA6 membrane is used as the outer layer. The design of the transparent additive‐free facial mask may serve as a guideline for developing new functional facial masks.
Tandem mass tag (TMT) quantitative protein analysis‐based proteomics and parallel reaction monitoring (PRM) validation revealed that MST4 accelerates osteosarcoma proliferation by increasing MRC2 activity
Authors: Liu Yunyan , Zhang Xiaoyu , Ren Xingguang , Sun Jin , Wen Yanhua , Guo Zheng , Ma Qiong 
Published: June 2023

AbstractOsteosarcoma is one of the most common orthopedic malignancies and is characterized by rapid disease progression and a poor prognosis. Currently, research on methods to inhibit osteosarcoma proliferation is still limited. In this study, we found that MST4 levels were significantly increased in osteosarcoma cell lines and tumor tissues compared to normal controls and demonstrated that MST4 is an influential factor in promoting osteosarcoma proliferation both in vivo and in vitro. Proteomic analysis was performed on osteosarcoma cells in the MST4 overexpression and vector expression groups, and 545 significantly differentially expressed proteins were identified and quantified. The candidate differentially expressed protein MRC2 was then identified using parallel reaction monitoring validation. Subsequently, MRC2 expression was silenced with small interfering RNA (siRNA), and we were surprised to find that this alteration affected the cell cycle of MST4‐overexpressing osteosarcoma cells, promoted apoptosis and impaired the positive regulation of osteosarcoma growth by MST4. In conclusion, this study identified a novel approach for suppressing osteosarcoma proliferation. Reduction of MRC2 activity inhibits osteosarcoma proliferation in patients with high MST4 expression by altering the cell cycle, which may be valuable for treating osteosarcoma and improving patient prognosis.
Self‐compassion facilitates responsiveness to existential threat: A brief report
Authors: Gerber Zach , Gez Lidar , Anaki David 
Published: June 2023

AbstractTerror management theory posits that validation of worldview and self‐esteem are primary defense mechanisms in keeping mortal concerns at bay, although potentially leading to the devaluation of others. Self‐compassion is considered a more inclusive defense mechanism. Drawing on preliminary research, we extended a previous investigation regarding the buffering role of self‐compassion within the Terror management theory paradigm. We embedded within a dot‐probe task a continuous death priming together with abstract and concrete threat images. We confirmed among 126 university students that following death priming, self‐compassion facilitated attentional attraction toward concrete threat and avoidance of abstract threat. These findings demonstrate the contribution of self‐compassion in coping simultaneously with different types of attentional threat and has potential to spawn future innovative research.