bluuebunny/crossref_metadata_2025_split huggingface dataset:
Based on transcriptome and metabolome analyses, a preliminary study on the causes of changes in paintings during Hibiscus syriacus flowering
Authors: LI Zhezhe , LIU Dan , WANG Dongsheng , SUN Meng , ZHANG Guojun , WU Yu , ZHANG Yidan , Cheng Bei-bei 
Published: August 2023

Abstract
Background
The flower color of H. syriacus 'Qiansiban' undergoes a transition from fuchsia to pink purple and finally to pale purple, thereby enhancing the ornamental value of the cultivars. However, the molecular mechanism underlying flower color change is still unclear in H. syriacus. In this study, the transcriptomic data ofH. syriacus 'Qiansiban' at five developmental stages were analyzed to investigate the impact of flavonoid components on flower color variation. Additionally, five cDNA libraries were constructed from H. syriacus 'Qiansiban' during critical blooming stages, and the transcriptome was sequenced to investigate the molecular mechanisms underlying changes in flower coloration.
Results
High-performance liquid chromatography‒mass spectrometry detected five anthocyanins in H. syriacus 'Qiansiban', with malvaccin-3-O-glucoside being the predominant compound in the flowers of H. syriacus at different stages, followed by petunigenin-3-O-glucoside. The levels of these five anthocyanins exhibit a gradual decline throughout the flowering process. In terms of the composition and profile of flavonoids and flavonols, a total of seven flavonoids were identified: quercetin-3-glucoside, luteolin-7-O-glucoside, Santianol-7-O-glucoside, kaempferol-O-hexosyl-C-hexarbonoside, apigenin-C-diglucoside, luteolin-3,7-diglucoside, and apigenin-7-O-rutinoside. A total of 2702 differentially expressed genes were identified based on the selected reference genome. Based on the enrichment analysis of differentially expressed genes, we identified 9 structural genes (PAL, CHS, FLS, DRF, ANS, CHI, F3H, F3'5'H, and UFGT) and 7 transcription factors (3 MYB, 4 bHLH) associated with flavonoid biosynthesis. The qRT‒PCR results were in good agreement with the high-throughput sequencing data.
Conclusion
This study will establish a fundamental basis for elucidating the mechanisms underlying alterations in the flower pigmentation of H. syriacus.
Synergistic Morphology and Feedback Control for Traversal of Unknown Compliant Obstacles with Aerial Robots
Authors: Aucone Emanuele , Geckeler Christian , Morra Daniele , Pallottino Lucia , Mintchev Stefano 
Published: August 2023

Abstract
Animals can traverse vegetation by direct physical interaction using their entire body to push aside and slide along compliant obstacles. Current drones lack this interaction versatility that stems from synergies between body morphology and feedback control modulated by sensing. Taking inspiration from nature, we show that a task-oriented design allows a drone with a minimalistic controller to traverse obstacles with unknown elastic responses. A discoid sensorized shell allows contact to be established and sensed anywhere along the shell and facilitates the sliding along the obstacle. This simplifies the formalization of the control strategy, which does not require a model of the interaction with the environments, nor high-level switching conditions for alternating between pushing and sliding, thereby reducing the computational burden. Indeed, we exploit an optimization-based controller that only ensures safety constraints on the state of the robot and dampens the oscillations of the environment during the interaction, even if the elastic response is unknown and variable. Experimental evaluation, using a hinged surface with three different stiffness values ranging from 18 to 155.5 Nmm/rad, validates the proposed strategy and the necessity for the synergic task-oriented design and feedback control. By also showcasing the traversal of real branches, this work makes an initial contribution toward drone flight across cluttered vegetation, with relevant applications in environmental monitoring, precision agriculture, and search and rescue.
Structural basis for pathogenic variants of GJB2 and hearing levels of patients with hearing loss
Authors: Namba Kazunori , Mutai Hideki , Matsunaga Tatsuo , Kaneko Hiroki 
Published: August 2023

Abstract

OBJECTIVES The crystal structure of the six protomers of gap junction protein beta 2 (GJB2) enables prediction of the effect(s) of an amino acid substitution, thereby facilitating investigation of molecular pathogenesis of missense variants of GJB2. This study mainly focused on R143W variant that causes hearing loss, and investigated the relationship between amino acid substitution and 3-D structural changes in GJB2.METHODS The R143W and structurally related variants of GJB2 were modeled using the crystal structure of GJB2 as a template. Patients with nonsyndromic hearing loss who appeared to have two GJB2 pathogenic variants, including the R143W variant, were investigated.RESULTS The predicted structure demonstrated that the hydrogen bond between R143 and N206 was important for the stability of the protomer structure. Also, R143W related N206S and N206T variants showed loss of the hydrogen bond.CONCLUSION Investigation of the genotypes and clinical data in patients carrying the R143W variant on an allele indicated that severity of hearing loss depends largely on the levels of dysfunction of the pathogenic variant on the allele, whereas a patient with the homozygous R143W variant demonstrated profound hearing loss. We concluded that the R143W variant causes structural destabilization of protein of GJB2.
Combined Exposure to Multiwalled Carbon Nanotubes and Dibutyl Phthalates Aggravated Airway Inflammation in Rats
Authors: He Suli , peng haiyan , Wu Min , Yan Chao , Wan Jian , ye xin , Zhang Hongmao , Ding Shumao 
Published: August 2023

Abstract
Previous work has shown that mice exposed to DBP-adsorbed onto MWCNTs, via tail vein injection, displayed black lesions in their lungs. To investigate the mechanism causing this toxicity in the lung tissue of rats, we performed an experiment with SD rats, exposing them to DBP adsorbed onto MWCNTs via a tail vein injection for 14 days. The results revealed pulmonary edema and greyish-black lung tissue in the MWCNTs and the MWCNTs + DBP combined exposure groups. In the combined exposure group there was evident alveolar fragmentation and adhesion, and lung tissue sections showed significant levels of black particles. Sections of the non-cartilaginous region of the trachea had significant folding of the pseudostratified ciliated columnar epithelium and marked thickening of the submucosa. In BALF, the number of WBC, Lym, Neu, and Eos cells, as well as levels of IgE, IL-6, TNF-α, and IL-1β, were all significantly higher. TNF-α, IL-6, STAT3, and α-SMA mRNA expression were all elevated in the lung tissue. The combined exposure group, which had considerable airway remodeling, had a greater degree of tracheal constriction and luminal narrowing, according to the results of the α-SMA immunofluorescence assay. According to these experimental findings, the exposure to both MWCNTs and DBP seemed to have a synergistic effect and exacerbated rats' impaired respiratory function that resulted from exposure to MWCNTs alone.
Genes and pathways underpinning Klinefelter syndrome at the single-cell level
Authors: Tian Linlin , Yu Yan , Mao Ziqing , Xu Dandan , Zhang Hongbo , Qiao Mengkai , Chen Tong , Liu Wen 
Published: August 2023

Abstract
Klinefelter syndrome (KS) is the most frequent genetic anomaly in infertile males. Despite this, the molecular mechanisms involved in KS are poorly defined. Based on bulk transcriptome and single-cell RNA sequencing datasets with peripheral blood monocyte (PBMC) sample(s) from healthy and KS men, this study was designed to address critical genes and pathways correlated with the occurrence of KS. Through a comparison between control and KS samples, we obtained 5 hub genes, including two upregulated genes (XG and ITLN1) and three downregulated genes (DEFA4, BPI and MPO). Without exception, these five genes yielded an excellent discriminatory capacity for KS with an area under the receiver-operator-characteristic curve over 0.75. We also assessed between-group differences of immune cell infiltration using ssGSEA. Infiltrated degree of some immune cells such as CD56bright NK cell was found to be positively associated with the expression of ITLN1 and XG. Through Kyoto Encyclopedia of Genes and Genomes enrichment, we identified PI3K/AKT pathway and neuroactive ligand-receptor interaction as upregulated pathways for KS. Gene set enrichment analysis together with gene set variation analysis confirmed upregulation of G2M checkpoint, mitotic spindle, and heme metabolism for KS. Furthermore, scRNA-seq data analysis was conducted to detect intercellular communication between different immune cell types, and a strong correlation was detected for macrophages, dendritic cells or NK cells with the other cell types. Collectively, we provided hub genes, pathways, immune cell infiltration degree, and cell-cell communication interactions for KS, warranting novel insights into the mechanisms of KS.
Multi-cell resonant boost converter based off-grid solar-PV system
Authors: Maurya Ajay Kumar , Das S. K. , Singh Dinesh Kumar , Chandra Isha 
Published: August 2023

Abstract
This paper presents an application of a multi-cell resonant boost converter as an MPPT device for solar PV off-grid systems. The paper also presents the comparison of the theoretical efficiency of a multi-cell resonant boost converter with a traditional boost converter used as an MPPT or high gain boost devices for solar PV – battery backup off-grid AC systems on various factors such as power, duty cycle, and frequency. It is concluded that a multi-cell resonant converter improves the system's overall efficiency by Zero Current Switching (ZCS). Furthermore, an energy management scheme is proposed for power generation management. The energy management scheme performs the following tasks: regulate power generation by MPP and non-MPP mode of operation, regulate power consumption by battery charging current control and load shedding, and ensure power supply to baseload (lean power demand). MATLAB 2021a is used for the simulation, and dSPACE DS1104 based controller is employed to run the experimental prototype. The simulation and experimental prototype results are presented to validate the theoretical concepts. The paper concludes that the multi-cell resonant boost converter is efficient, and performs successfully at various power regulation tasks therefore, the multi-cell resonant boost converter is a better choice over a traditional boost converter for solar-PV off-grid systems.
Improving Service Access for Under-resourced Parents of Children on the Autism Spectrum: a Randomized Controlled Trial
Authors: Hassrick Elizabeth McGhee , Iadarola Suzannah , Pellecchia Melanie , Gulsrud Amanda , Shih Wendy , Lee Hyon Soo , Vejnoska Sarah , Fitzgerald Amber , León-Thomas Mariela , Hochheimer Samantha , Straiton Diondra , Luelmo Paul , Morgan Elizabeth , Nuske Heather , Li Jennica , Garcia Consuelo , Yu Yue , Cho EunMi , Wright Aziza , Mandell David , Kasari Connie , Stahmer Aubyn 
Published: August 2023

Abstract
Background
Historically marginalized and minoritized families experience disparities in autism identification and access to services. Few interventions are co-designed in partnership with under-resourced families and the service providers who support them to help families successfully navigate the autism services system. Little is known about how to create new network connections between historically marginalized parents and professionals.
Methods
Using a community partnered participatory research model, we developed Mind the Gap (MTG), a peer navigation intervention that supports historically marginalized and minoritized families in setting goals and engaging in activities to access services for their recently diagnosed child on the autism spectrum. We compared the effects of MTG (n = 53) on randomly assigned participants to a resources-only comparison (n = 59) in a four-site randomized controlled trial. Participants were recruited from lower-resourced communities using partnered participatory research methods, with 92% of families enrolled in the study earned less than USD $50,000 per year. Our primary outcome was caregiver service access over time and our secondary outcomes were caregiver reported family and professional support networks, and family, community, and services empowerment.
Results
In our intent-to-treat analysis, MTG caregivers did not differ in service access relative to the comparison group; however, MTG caregivers had increased professional networks and improved service empowerment post intervention relative to the comparison group.
Conclusion
Results suggest this low-cost intervention can improve the support networks and service empowerment for caregivers at high risk of service disconnections and may empower their accessing other services in the future.
Clinical Trial Registration
NCT03711799; Registration Date: 10/12/2018 https://clinicaltrials.gov/ct2/show/NCT03711799?type=Intr&cond=Autism&intr=Gap&age=0&draw=2&rank=2
Real-time Energy-efficient Sensor Libraries for Wearable Devices
Authors: Calisti Lorenzo , Lattanzi Emanuele 
Published: August 2023

Abstract

The growing popularity of wearable technology has led to a surge in smartwatch usage among the general public. These devices offer a range of features, including internet connectivity, fitness tracking, and real-time notifications, making them valuable tools for staying connected to the online world while remaining engaged in real-world activities.
Smartwatches have become powerful platforms for Human Activity Recognition (HAR) applications thanks to the increasing computational power and the presence of a wide array of sensors, such as accelerometers, gyroscopes, heart rate, and step counters.
Efficient real-time data collection from internal sensors is a crucial requirement for HAR applications on wearable devices due to their constraints in battery size and duration.
In this paper, we introduce the implementation of three energy-efficient user-level libraries developed for real-time data collection from inertial sensors using native Wear OS APIs and different techniques: Thread, Flow, and Channel.
Experiments were conducted on a commercially available Oppo smartwatch comparing them in terms of code size, memory utilization, and energy consumption.
The characterization results demonstrate empirically that the solution is lightweight and adaptable, making it suitable for development on wearable devices without significant impact on battery life and system performance.
Prenatal Exposure to Gestational Diabetes Mellitus is Associated with Mental Health Outcomes and Physical Activity has a Modifying Role
Authors: Alves Jasmin M. , Smith Alexandro , Chow Ting , Negriff Sonya , Carter Sarah , Xiang Anny H. , Page Kathleen A. 
Published: August 2023

Abstract
Background
Studies suggest a link between prenatal gestational diabetes mellitus (GDM) exposure and poor mental health outcomes. We examined associations between prenatal GDM exposure and depressive and anxiety symptoms in children and assessed physical activity as a potential modifier of these associations.
Method
Seventy children (AgeM(SD): 12(2.0), 56% GDM, 59% female) and their parents completed surveys: Center for Epidemiological Studies Depression Scale for Children (CES-DC), State-Trait Anxiety Inventory for Children (STAIC), Child Behavior Checklist (CBCL), and 3-day physical activity recall (3DPAR). Associations between mental health measures with GDM exposure and interactions between GDM exposure and child moderate-to-vigorous physical activity (MVPA) were assessed using regression.
Results
GDM-exposed children had higher anxiety (p = 0.03) and internalizing symptoms (CBCL) (p = 0.04) than unexposed children. There was an interaction between GDM exposure and child MVPA on anxiety (p = 0.02), internalizing (p = 0.04) and externalizing symptoms (p = 0.004). In the low MVPA group, GDM exposed children had more depressive (p = 0.03), anxiety (p = 0.003), and internalizing symptoms (p = 0.03) than unexposed children. In the high MVPA group, there were no group differences except with externalizing symptoms (p = 0.04).
Conclusion
Prenatal GDM is associated with higher anxiety and internalizing symptoms in children. Child MVPA modified the relationship between GDM exposure and mental health outcomes suggesting that physical activity during childhood could mitigate the negative mental health outcomes associated with prenatal GDM exposure.
LysipheN : A gravimetric IoT device for Near Real-time High-Frequency Crop Phenotyping: a case study on common beans
Authors: Pineda-Castro Duvan , Diaz Harold , Soto Jonatan , Urban Milan Oldřich 
Published: August 2023

Abstract
Climate instability directly affects agro-environments. Water scarcity, high air temperature, and changes in soil biota are some factors caused by environmental changes. Verified and precise phenotypic traits are required for assessing the impact of various stress factors on crop performance while keeping phenotyping costs at a reasonable level. Experiments which use a lysimeter method to measure transpiration efficiency are often expensive and require complex infrastructures. This study presents the development and testing process of an automated, reliable, small, and low-cost prototype system using IoT with high-frequency potential in near-real time. Because of its waterproofness, our device - LysipheN - assesses each plant individually and can be deployed for experiments in different environmental conditions (farm, field, greenhouse, etc.). LysipheN integrates multiple sensors, automatic irrigation according to desired drought scenarios, and a remote, wireless connection to monitor each plant and device performance via a data platform. During testing, LysipheN proved to be sensitive enough to detect and measure plant transpiration, from early to ultimate plant developmental stages. Even though the results were generated on common beans, the LysipheN can be scaled up/adapted to other crops. This tool serves to screen transpiration, transpiration efficiency, and transpiration-related physiological traits. Because of its price, endurance, and waterproof design, LysipheN will be useful in screening populations in a realistic ecological and breeding context. It operates by phenotyping the most suitable parental lines, characterizing genebank accessions, and allowing breeders to make a target-specific selection using functional traits (related to the place where LysipheN units are located) in line with a realistic agronomic background.
Prevalence of asymptomatic malaria at the communal level in Burkina Faso: an application of the small area estimation approach
Authors: BASSINGA Hervé , OUEDRAOGO Mady , CISSE Kadari , YIRA Parfait , OUEDRAOGO Clement Sibiri , NOMBRE Abdou , BANCE Wofom Lydie Marie-Bernard , KUEPIE Mathias , ROUMBA Toussaint 
Published: August 2023

Abstract

Background
In malaria-endemic countries, asymptomatic malaria carriers represent an important reservoir for malaria transmission. Estimating the burden at a fine scale and identifying areas at high risk of asymptomatic carriage are important to guide malaria control strategies. This study aimed to estimate the prevalence of asymptomatic malaria at the communal level, the smallest geographical entity from which a local development policy can be driven.
Methods
The data used in this study came from several sources: the 2018 Multiple Indicator Cluster Survey on Malaria and the 2019 census data and environmental data available from open sources. The analysis involved a total of 5,489 children under 5 from the malaria survey and 293,715 children under 5 from the census. The estimation approach used is that of Elbers Langjouw and Langjouw (ELL). This approach consists of including data from several sources, mainly census and survey data, in a statistical model to obtain predictions at a geographical level below that of the survey, for indicators that are not measured in the population census. The method achieves this by finding correlations between common census variables and survey data.
Findings
The findings suggest that the spatial distribution of the prevalence of asymptomatic malaria infection is very heterogeneous across the communes. It varies from a minimum of 5.1% (with confidence interval IC95%= [3.6 ; 6.5]) in the commune of Bobo-Dioulasso to a maximum of 41.4% (IC95%=[33.5 ; 49.4]) in the commune of Djigoué. Of the 341 communes, 208 (61%) had prevalences above the national average of 20.3% (IC95%= [18.8; 21.2]).
Contribution
This analysis provided commune-level estimates of the prevalence of asymptomatic malaria in Burkina Faso. The results of this analysis should help to improve planning of malaria control at the communal level in Burkina Faso.
How many cases do instructor class pediatric surgeons need to experience to be an independent operator in performing advanced endoscopic surgery? - A nationwide survey to establish an ideal curriculum for pediatric endoscopic surgery in Japan
Authors: Murakami Masakazu , Onishi Shun , Yamada Koji , Ogawa Katsuhiro , Yokoyama Shinichiro , Kurashima Yo , Miyano Go , Ishimaru Tetsuya , Kawashima Hiroshi , Uchida Hiroo , Yamataka Atsuyuki , Okuyama Hiroomi , Ieiri Satoshi 
Published: August 2023

Abstract
Purpose: To ensure the safe spread of pediatric endoscopic surgery, it is essential to build a training curriculum, and a survey of the current situation in Japan is necessary. The present study assessed an efficient training curriculum by clarifying instructor class pediatric surgeons’ experiences, including autonomy when performing advanced endoscopic surgeries.
Methods: An online nationwide questionnaire survey was conducted among pediatric surgeons who had Endoscopic Surgical Skill Qualification (ESSQ) and board-certified instructors who had skills comparable to ESSQ. We assessed participants’ training experience, opinions concerning the ideal training curriculum and the correlation between surgical experience and the level of autonomy. The Zwisch scale was used to assess autonomy.
Results: Fifty-two participants responded to the survey (response rate: 86.7%). Only 57.7% of the respondents felt that they had received sufficient endoscopic surgery training. Most respondents considered an educational curriculum for endoscopic surgery including off-the-job training essential during the training period. Autonomy had been acquired after experiencing two to three cases for most advanced endoscopic surgeries.
Conclusion: This first nationwide survey in Japan showed that instructor class pediatric surgeons acquired autonomy after experiencing two to three for most advanced endoscopic surgeries. Our findings suggest that training, especially off-the-job training, has been insufficient.
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