bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ophiobolin A Covalently Targets Complex IV Leading to Mitochondrial Metabolic Collapse in Cancer Cells
Authors: Gowans Flor A. , Thach Danny Q. , Wang Yangzhi , Altamirano Poblano Belen E. , Dovala Dustin , Tallarico John A. , McKenna Jeffrey M. , Schirle Markus , Maimone Thomas J. , Nomura Daniel K. 
Published: March 2023

SummaryOphiobolin A (OPA) is a sesterterpenoid fungal natural product with broad anti-cancer activity. While OPA possesses multiple electrophilic moieties that can covalently react with nucleophilic amino acids on proteins, the proteome-wide targets and mechanism of OPA remain poorly understood in many contexts. In this study, we used covalent chemoproteomic platforms to map the proteome-wide reactivity of OPA in a highly sensitive lung cancer cell line. Among several proteins that OPA engaged, we focused on two targets—cysteine C53 of HIG2DA and lysine K72 of COX5A—that are part of complex IV of the electron transport chain and contributed significantly to the anti-proliferative activity. OPA activated mitochondrial respiration in a HIG2DA and COX5A-dependent manner, led to an initial spike in mitochondrial ATP, but then compromised mitochondrial membrane potential leading to ATP depletion. We have used chemoproteomic strategies to discover a unique anti-cancer mechanism of OPA through activation of complex IV leading to compromised mitochondrial energetics and rapid cell death.
Plasma NT1-tau and Aβ42correlate with age and cognitive function in two large Down syndrome cohorts
Authors: Stern Andrew M. , Van Pelt Kathryn L. , Liu Lei , Anderson Amirah K. , Ostaszewski Beth , Mapstone Mark , O’Bryant Sid , Petersen Melissa E. , Christian Bradley T. , Handen Benjamin L. , Selkoe Dennis J. , Schmitt Frederick , Head Elizabeth ,   the Alzheimer’s Biomarker Consortium – Down Syndrome (ABC-DS) investigators
Published: March 2023

Structured AbstractIntroductionPeople with Down syndrome (DS) often develop Alzheimer disease (AD). Here we asked whether ultrasensitive plasma immunoassays for a tau N-terminal fragment (NT1-tau) and Aβ isoforms predict cognitive impairment.MethodsPlasma NT1-tau, Aβ37, Aβ40, and Aβ42levels were measured in a longitudinal discovery cohort (N = 85 participants, 220 samples) and a cross-sectional validation cohort (N = 239). We developed linear models and predicted values in the validation cohort.ResultsLinear mixed models for NT1-tau, Aβ42,and Aβ37:42were significant for age, there was no main effect of time in the discovery cohort. In cross-sectional models, NT1-tau and Aβ42increased with age. NT1-tau predicted DLD scores. The discovery cohort linear model for NT1-tau predicted NT1-tau levels in the validation cohort.DiscussionNT1-tau correlates with age and worse cognition in DS. Further validation of NT1-tau and other plasma biomarkers of AD neuropathology in DS cohorts is important for clinical utility.
Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that bind a conserved membrane proximal epitope of the spike
Authors: De Cae Sieglinde , Van Molle Inge , van Schie Loes , Shoemaker Sophie R. , Deckers Julie , Debeuf Nincy , Lameire Sahine , Nerinckx Wim , Roose Kenny , Fijalkowska Daria , Devos Simon , Desmet Anne-Sophie , Zavala Marchan Jackeline Cecilia , Venneman Toon , Sedeyn Koen , Ballegeer Marlies , Vanheerswynghels Manon , De Wolf Caroline , Demol Hans , Vanhaverbeke Pieter , Ghassabeh Gholamreza Hassanzadeh , Lonigro Chiara , Bockstal Viki , Rinaldi Manuela , Abdelnabi Rana , Neyts Johan , Marqusee Susan , Lambrecht Bart N. , Callewaert Nico , Remaut Han , Saelens Xavier , Schepens Bert 
Published: March 2023

AbstractCurrently circulating SARS-CoV-2 variants have gained complete or significant resistance to all SARS-CoV-2-neutralizing antibodies that have been used in the clinic. Such antibodies can prevent severe disease in SARS-CoV-2 exposed patients for whom vaccines may not provide optimal protection. Here, we describe single-domain antibodies (VHHs), also known as nanobodies, that can broadly neutralize SARS-CoV-2 with unusually high potency. Structural analysis revealed their binding to a unique, highly conserved, membrane proximal, quaternary epitope in the S2 subunit of the spike. Furthermore, a VHH-human IgG1 Fc fusion, efficiently expressed in Chinese hamster ovary cells as a stable antibody construct, protected hamsters against SARS-CoV-2 replication in a therapeutic setting when administered systemically at low dose. This VHH-based antibody represents a new candidate anti-COVID-19 biologic that targets the Achilles heel of the viral spike.
Numerical investigation of active flow control using zero-net-mass-flux jets around a high-lift morphing cambered wing-flap system
Authors: Truong Hung , Marouf Abderahmane , Gehri Alain , Vos Jan , Braza Marianna , Hoarau Yannick 
Published: March 2023

Purpose
This study aims to investigate the physical mechanisms of the use of active flow control (AFC) for a high-lift wing-flap configuration.

Design/methodology/approach
By means of high-fidelity numerical simulations, the flow dynamics around a high-lift wing-flap system at high Reynolds number (Re/c = 4.6 million) is studied. Adapted turbulence models based on the URANS approach are used to capture the flow separation and the subsequent development of coherent structures. The present study focuses on the use of AFC using a synthetic jet known as zero-net-mass-flux (ZNMF) using the blowing–suction approach. Different parameters (geometry, frequency and velocity) of a ZNMF placed at the cambered flap’s chord are optimized to obtain the most efficient parameter settings to suppress the flow separation.

Findings
A synthetic jet with the optimal shape and orientation enforces the flow reattachment on the wing-flap surface. This leads to an improvement of the aerodynamic performance of the system. The wake thickness was reduced by 30%, and an increase of 17.6% in lift-to-drag ratio was obtained. Concerning the ZNMF location, they should be installed upstream of the separation point to achieve the best performance.

Originality/value
The effectiveness of ZNMF devices integrated on a high-lift wing-flap configuration was studied in real flight conditions at high Reynolds number. A detailed analysis of the wake dynamics explains how AFC forces the reattachment of the boundary layer and attenuates the predominant wake instabilities up to −20 dB.
Relationship clusters and performance of conflict management strategies in cross-organisational projects teams
Authors: Kwofie Titus Ebenezer , Ellis Florence Akyaa , Addy Michael Nii , Amos-Abanyie Samuel , Aigbavboa Clinton , Afram Samuel Owusu 
Published: March 2023

PurposeThe link between relationship typologies and effectiveness of conflict resolution approaches remains to be tested despite its significance in conflict management in construction project delivery. By using the four relationships attributes based on the group attachment theory, the purpose of the study was to explore the cluster of relationships among project teams and organisations and the performance of conflict management strategies across these clusters in the Ghanaian construction industry.Design/methodology/approachUsing a deductive questionnaire survey in the Ghanaian construction industry, a total of 137 responses were gathered and analyzed using cluster analysis, mean scores and ANOVA to reveal the relationship clusters and performance of conflict management strategies across these clusters.FindingsThe results revealed eight relationship clusters that exist among project teams and organisations with distinct influence of roles & tasks function, cognition, emotions and behavior attributes across the relationship clusters. In the aspect of the effectiveness of conflict management strategies, it was noted that the performance of these strategies were significantly different across the groups. For instance, integrating as a conflict management strategy was deemed to be effective in resolving conflict in unitary, adversarial, pluralist, mutuality, collaborative and partnering relationship clusters. In the case of coopetitive and coercive relationships, the performance of integrating as a conflict management strategy was less effective. This study thus has empirically proved that, different relationship clusters of teams and organizations exist within the Ghanaian construction industry, and that they perform different roles & tasks functions, cognition, emotions and behavioural attributes in their formation. Additionally, the performance effectiveness of conflict management strategies differed across the relationship clusters.Originality/valueBy aligning the relationship attributes to the dynamics of relationship clusters experienced in project teams and organisations, relationship quality, suitability and effectiveness of conflict management strategies can be optimized. The findings can inform project teams and stakeholders to develop fit-for-purpose relationship attributes among teams and organisations to enhance team effectiveness, relationship quality and conflict management in the industry.
Relational quality in public–private partnerships: understanding social relationships in contract-based exchanges
Authors: Warsen Rianne 
Published: March 2023

PurposeThe purpose of this paper is to conduct a systematic literature review of studies addressing relational quality in public–private partnerships (PPPs). The ambition of this study is twofold: first, to present more clarity regarding the definition of the concept. Second, to develop a framework that explains the antecedents, characteristics and outcomes of relational quality in PPPs.Design/methodology/approachA systematic literature review of 99 academic articles, which were analyzed and coded on the definition, characteristics, antecedents and outcomes of relational quality.FindingsThe study shows that trust, communication, commitment, openness and reciprocity are considered core aspects of the concept. The analysis further identifies important antecedents (e.g. shared values, leadership) and outcomes (performance, innovation) of relational quality in PPPs. The findings result in the development of a framework on relational quality in PPPs to be used for further research.Research limitations/implicationsThe findings indicate that relational quality is valuable for the functioning of PPPs, but the current focus of this review excludes studies addressing relational quality in other types of collaboration and public–private exchange. The lessons from these research fields might further improve people’s understanding of relational quality.Practical implicationsPractitioners should pay attention to relational quality in long-term PPPs. Among others, (process) management activities might contribute to relational quality as it stimulates communication and openness.Originality/valueThe presented framework explaining antecedents, characteristics and outcomes of relational quality allows for more structured use of this concept in future research.
Anomaly detection in the course evaluation process: a learning analytics–based approach
Authors: Vaidya Anagha , Sharma Sarika 
Published: March 2023

PurposeCourse evaluations are formative and are used to evaluate learnings of the students for a course. Anomalies in the evaluation process can lead to a faulty educational outcome. Learning analytics and educational data mining provide a set of techniques that can be conveniently applied to extensive data collected as part of the evaluation process to ensure remedial actions. This study aims to conduct an experimental research to detect anomalies in the evaluation methods.Design/methodology/approachExperimental research is conducted with scientific approach and design. The researchers categorized anomaly into three categories, namely, an anomaly in criteria assessment, subject anomaly and anomaly in subject marks allocation. The different anomaly detection algorithms are used to educate data through the software R, and the results are summarized in the tables.FindingsThe data points occurring in all algorithms are finally detected as an anomaly. The anomaly identifies the data points that deviate from the data set’s normal behavior. The subject which is consistently identified as anomalous by the different techniques is marked as an anomaly in evaluation. After identification, one can drill down to more details into the title of anomalies in the evaluation criteria.Originality/valueThis paper proposes an analytical model for the course evaluation process and demonstrates the use of actionable analytics to detect anomalies in the evaluation process.
On the dynamic relationship between transaction volume and returns: evidence from the cryptocurrency market
Authors: Ghabri Yosra , Gana Marjène Rabah 
Published: March 2023

PurposeUsing vector autoregressive modelling (VAR) and Granger causality tests, this paper attempts to empirically investigate the dynamic relationship between return and volume of transactions of two main cryptocurrencies: Bitcoin and Ethereum.Design/methodology/approachBased on a generalized autoregressive conditional heteroskedasticity (GARCH) model with a transaction volume parameter in the conditional volatility equation.FindingsThe results provide empirical evidence of a positive contemporaneous relationship between the variation in transaction volume and the daily return of Bitcoin and Ethereum. The results also show that the conditional volatility of the returns is affected by the past volatility, which implies weak-form inefficiency for both Bitcoin and Ethereum markets. The results of the VAR model, testing Granger causality, indicate that the volume of transactions Granger-Causes Bitcoin and Ethereum returns. Furthermore, the findings show a Granger causal relation from returns to volume.Originality/valueThis result suggests that cryptocurrency returns can predict transaction volumes and vice versa.
Automation, the growth of online information and digital formats: the story of libraries
Authors: Frederick Donna Ellen 
Published: March 2023

Purpose
The purpose of this paper is to examine the impact of technological change, the data deluge, social change and policy change on the materials in their collection, the services they offer and how service is provided.

Design/methodology/approach
The author examines over four decades of personal experience as a library user and library worker to discover the progression of library resources, reference practices and the storage of information from analogue to digital.

Findings
Since the 1970s, there has been a gradual shift away from analogue technologies towards digital ones. One exception is that of the low-tech analogue book which, while it is not as central as it once was, remains important for most library collections. The change was and is driven by internal and external pressures and can be impeded by factors such as financial and skill limitations or decision makers who do not support changes.

Originality/value
While the Data Deluge Column has many instalments that examine problems created by the growing mass of data and information and the progression to technological change, this instalment puts all of that into a human or lived perspective.
Review article: Mechanisms underlying the effectiveness of exclusive enteral nutrition in Crohn's disease
Authors: Melton Sarah L. , Taylor Kirstin M. , Gibson Peter R. , Halmos Emma P. 
Published: March 2023

SummaryBackgroundExclusive enteral nutrition (EEN) induces remission and mucosal healing in patients with Crohn's disease, but the mechanism of action remains unknown.AimTo outline current understanding of the mechanisms of action of EEN.MethodsFrom a comprehensive literature search, published data were critically examined in a narrative review.ResultsMultiple potential mechanisms of action have been identified. EEN optimises nutritional status. Differences in gut microbiota in terms of overall diversity and taxonomic community structure are observed between responders and non‐responders to EEN. Therapy with EEN alters microbial metabolites (including faecal short‐chain fatty acids, amino acids, branched‐chain amino acids and sulphide) and faecal pH. Epithelial effects and restoration of barrier function, as well as changes in mucosal cytokine profiles and T‐cell subsets are observed in responders to EEN. The impact of inclusion or exclusion of specific dietary components may be of importance, but putative detrimental components are found in many formulas. A major challenge in interpreting these findings is that they often contradict or change in opposite directions to what is considered ‘beneficial’. It is difficult to differentiate between the observations following EEN being driven by EEN per se and those associated with resolving inflammation.ConclusionsThe mechanisms of action of EEN are likely to involve a complex interplay between host mucosal immune response and luminal environment, but the identity of key factors remains poorly understood. A better definition of pathogenic factors may aid in developing more targeted dietary treatment and provide insights into the pathogenesis of Crohn's disease.
L'agrandissement de la communauté Uashat mak Mani‐utenam : regard sur une collaboration dans la durée entre une université et une communauté innue
Authors: Gouin Élisa 
Published: March 2023

RésuméCet article présente une étude de cas ayant pour but de cerner les facteurs de succès et les effets d'une collaboration entre des étudiants en architecture et une communauté autochtone. Cet exercice, qui s'inscrit dans le contexte d'une relation collaborative de plusieurs années, a été effectué dans le cadre d'un atelier en design urbain mené par l'École d'architecture de l'Université Laval et la communauté innue de Uashat mak Mani‐utenam, voisine de la ville de Sept‐Îles, au Québec. L'agrandissement de la communauté et l'aménagement de zones résidentielles étaient au cœur de la démarche de collaboration. L'analyse de l'évolution des différentes versions des plans d'aménagement, à la lumière de critères d'aménagement innus, met en exergue les apports positifs de l'atelier, notamment en ce qui concerne le programme communautaire et le symbolisme tectonique. Néanmoins, le statu quo est maintenu relativement à l'implantation et au respect des éléments naturels. Les témoignages d'acteurs clefs impliqués dans le projet illustre le caractère authentique du partenariat de recherche entre le groupe de recherche « Habitats et cultures » et la communauté. La qualité de la relation entre les chercheurs et les partenaires innus, la durée du partenariat dans le temps et la valorisation des savoirs culturels innus ont favorisé le succès du partenariat.
Introduction to the special section on Biology of Termites
Authors: Xing Lian‐Xi , Beukeboom Leo W. 
Published: March 2023

AbstractAs descendants of social cockroaches, termites have an intriguing biology that makes them highly suitable study subjects in a wide variety of fields, such as eusocial evolution, ageing mechanisms, wood decomposition, ecosystem engineering, and pest management. This special section presents four studies ranging from the role of chemical compounds in foraging behaviour and food digestion to genetics and physiology of reproduction. Taken together, these studies offer new insights and ample suggestions for future work on these fascinating insects.
Current anthropogenic warming threatens shallow Lake ecosystems on the Tibetan plateau: A palaeolimnological study covering the past 1,600 years in Genggahai Lake
Authors: Li Yuan , Zhao Hui , Sun Mingjie , Zhao Yongtao , Jeppesen Erik 
Published: March 2023

Abstract

The Tibetan Plateau has ∼1,200 lakes larger than 1 km2 with a total area of ~46,000 km2. The annual mean air temperature of the Tibetan Plateau has increased 2.5°C over the past 60 years. Lakes in this region are extremely responsive to climate changes as a result of their low biodiversity and simple food webs. However, it is unclear whether the current anthropogenic warming will benefit or threaten the aquatic ecosystems in this region.

We assessed patterns of change associated with climate change in a shallow, freshwater lake (Genggahai Lake) on the NE Tibetan Plateau over the past ~1,600 years based on diverse aquatic fossils (macrophytes, molluscs, cladocerans and diatoms), total organic carbon (TOC) and total nitrogen (TN) of a sediment core.

The abundances of macrophytes, molluscs and cladocerans, and the concentrations of TOC and TN in the sediments were high during the Mediaeval Warm Period (MWP, ~940–1,410 CE) but low during the Dark Ages Cold Period (DACP, ~390–940 CE) and the Little Ice Age (LIA, ~1,410–1970 CE), suggesting that modest natural warming was associated with the biomass of macrophytes and consumers in the food web. By contrast, low abundance of macrophytes and high abundance of planktonic diatoms were recorded in the sediments during the Current Warm Period (CWP, ~1970–2020 CE). Current anthropogenic warming has resulted in significantly higher temperatures than during the MWP, associated with the development of planktonic algae rather than macrophytes.

Our findings show that the current anthropogenic warming, coupled with increased atmospheric nitrogen deposition and activated nutrients from thawed permafrost, is associated with phytoplankton dominance in lakes on the Tibetan Plateau, and may lead to significant reductions in ecosystem services provided by the lakes.
Cerebrospinal fluid virology in people with HIV
Authors: Henderson Merle , Pepper Nuala , Bawa Manraj , Muir David , Everitt Alex , Mackie Nicola E. , Winston Alan 
Published: March 2023

AbstractObjectiveOur objectives were to investigate the recent frequency of cerebrospinal fluid (CSF) HIV RNA escape and other CSF viral nucleic acid detection in people with HIV with neurological symptoms and to assess associated clinical factors.MethodThis was a retrospective cohort analysis of people with HIV who underwent CSF examination for clinical indications between 2017 and 2022. Individuals were identified from pathology records, and clinical data were recorded. CSF HIV RNA escape was defined as CSF HIV RNA concentrations greater than in plasma. CSF viral screen included herpes simplex virus types 1 (HSV‐1) and 2 (HSV‐2), varicella zoster virus (VZV), Epstein Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV‐6) and JC virus. When cases were detected in five or more people with HIV, associated clinical factors were assessed using linear regression modelling.ResultsCSF HIV RNA escape was observed in 19 of 114 individuals (17%) and was associated with the presence of HIV drug resistance mutations and non‐integrase strand transfer inhibitor‐based antiretroviral therapy (p < 0.05 for all) when compared to people with HIV without escape. Positive viral nucleic acid testing included EBV (n = 10), VZV (3), CMV (2), HHV‐6 (2) and JC virus (4). Detectable CSF EBV was not considered related to neurological symptoms and was associated with concomitant CSF infections in eight of ten individuals and with CSF pleocytosis, previous AIDS, lower nadir and current CD4 T‐cell count (p < 0.05 for all).ConclusionIn people with HIV with neurological symptoms, the frequency of CSF HIV RNA escape remains similar to that in historical reports. Detectable EBV viral nucleic acid in the CSF was observed frequently and, in the absence of clinical manifestations, may be a consequence of CSF pleocytosis.