bluuebunny/crossref_metadata_2025_split huggingface dataset:
The thin descending limb of the loop of Henle originates from proximal tubule cells during mouse kidney development
Authors: Chung Eunah , Nosrati Fariba , Adam Mike , Potter Andrew , Sayed Mohammed , Humphreys Benjamin D. , Lim Hee-Woong , Hu Yueh-Chiang , Potter S. Steve , Park Joo-Seop 
Published: January 2025

AbstractBackgroundThe thin descending limb (DTL) of the loop of Henle is crucial for urine concentration, as it facilitates passive water reabsorption. Despite its importance, little is known about how DTL cells form during kidney development. Single-cell RNA sequencing (scRNA-seq) studies have not definitively identified DTL cells in the developing mouse kidney.MethodsWe assembled a large scRNA-seq dataset by integrating multiple datasets of non-mutant developing mouse kidneys to identify developing DTL cells. To test whether DTL cells originate from proximal tubule (PT) cells, we generated a PT-specific Cre line,Slc34a1eGFPCre, and conducted lineage tracing of PT cells. Additionally, given that the transcription factor Hnf4a directly binds to theAqp1gene, we examined whether the loss of Hnf4a affectsAqp1expression in DTL cells.ResultsFrom our scRNA-seq dataset, we identified a small cluster of cells distinct from both the proximal tubule and the thick ascending limb of the loop of Henle. Those cells exhibited high expression of DTL marker genes, includingAqp1andBst1. Notably, a subset of PT cells also expressed DTL marker genes, suggesting that PT cells may give rise to DTL cells. Using lineage tracing with theSlc34a1eGFPCreline, we found that DTL cells were positive for the Rosa26 reporter, confirming that DTL cells are descendants of PT cells. Furthermore, the loss of Hnf4a, a transcription factor essential for mature PT cell formation, disrupted properAqp1expression in DTL cells, providing additional evidence of a developmental link between PT cells and DTL cells.ConclusionOur findings shed new light on the developmental origin of DTL cells and highlight the importance of Hnf4a in regulating their formation.Key PointsReference single cell RNA-seq dataset of the developing mouse kidney was assembled and used to identify the thin descending limb of the loop of Henle.Lineage analysis of proximal tubules in the mouse kidney shows that proximal tubule cells give rise to the thin descending limb of the loop of Henle.Deletion of Hnf4a disrupts the expression ofAqp1in the thin descending limb of the loop of Henle, highlighting a developmental link between proximal tubules and the loop of Henle.
Monitoring monomer-specific acyl-tRNA levels in cells with PARTI
Authors: Pressimone Meghan A. , Schissel Carly K. , Goss Isabella H. , Swenson Cameron V. , Schepartz Alanna 
Published: January 2025

ABSTRACTWe describe a new assay that reports directly on the acylation state of a user-chosen tRNA in cells. We call this assay 3-Prime Adenosine-Retaining Aminoacyl-tRNA Isolation (PARTI). It relies on high-resolution mass spectrometry identification of the acyl-adenosine species released upon RNase A cleavage of isolated cellular tRNA. Here we develop the PARTI workflow and apply it to understand three recent observations related to the cellular incorporation of non-α-amino acid monomers into protein: (1) the origins of the apparent selectivity of translation with respect to β2-hydroxy acid enantiomers; (2) the activity of PylRS variants for benzyl derivatives of malonic acid; and (3) the apparent inability ofN-Me amino acids to function as ribosome substrates in living cells. Using the PARTI assay, we also provide direct evidence for the cellular production of 2’,3’-diacylated tRNA in certain cases. The ease and simplicity of the PARTI workflow should benefit ongoing efforts to study and improve the cellular incorporation of non-α-amino acid monomers into proteins.GRAPHICAL ABSTRACT
Standardized and calibrated light stimuli via head-mounted displays for investigating the non-visual effects of light
Authors: Fernandez-Alonso Maydel , Spitschan Manuel 
Published: January 2025

AbstractLight influences human physiology profoundly, affecting the circadian clock and suppressing the endogenous hormone melatonin. Experimental studies often employ either homogenous full-field stimulation, or overhead illumination, which are hard to standardize across studies and laboratories. Here, we present a novel technique to examine non-visual responses to light using virtual-reality (VR) head-mounted displays (HMDs) for delivering standardized and calibrated light stimuli to observers in a reproducible and controlled fashion. We find that VR HMDs are well-suited for delivering standardized stimuli defined in luminance and across time, with excellent properties up to 20 Hz. We examine melatonin suppression to continuous luminance-defined light stimuli in a sample of healthy participants (n=31, mean±SD age: 27.4±5.6), and find robust melatonin suppression in 27 out of 31 participants (87% of the sample). Our findings demonstrate that VR HMDs are well-suited for studying the mechanisms underlying human non-visual photoreception in a reproducible and standardized fashion.
The rice ethylene receptor OsERS1 negatively regulates the shoot growth and salt tolerance in rice seedlings
Authors: Morita Shigeto , Tanaka Soma , Tani Yuzuki , Nakamura Jun’ichi , Sato Masa H. , Satoh Shigeru , Masumura Takehiro 
Published: January 2025

AbstractEthylene is a gaseous plant hormone that regulates various aspects of growth and development. It promotes shoot growth in etiolated rice seedlings, which is crucial for seedling establishment and has significant agricultural implications. Of the five ethylene receptors in rice,OsERS1is the most abundantly expressed in seedlings. Therefore, we investigated the localization and role of OsERS1 in shoot growth. When expressed in rice protoplasts, OsERS1 was localized in the endoplasmic reticulum. Knockout mutants ofOsERS1exhibited enhanced shoot growth in etiolated seedlings under normal and ethylene-treated conditions. Additionally, the induction of ethylene-responsive genes was increased in the mutants, indicating an elevated ethylene response due toOsERS1knockout. The mutants also showed increased salt tolerance. In conclusion, our findings suggest that OsERS1 negatively regulates shoot growth and salt tolerance in etiolated rice seedlings.
Measurement of the electric field distribution in streamer discharges
Authors: Guo Yihao , Limburg Anne , Laarman Jesse , Teunissen Jannis , Nijdam Sander 
Published: January 2025

Using electric field induced second harmonic generation (E-FISH), we performed direction-resolved absolute electric field measurements on single-channel streamer discharges in 70 mbar (7 kPa) air with 0.2 mm and 2 ns resolutions. In order to obtain the absolute (local) electric field, we developed a deconvolution method taking into account the phase variations of E-FISH. The acquired field distribution shows good agreement with the simulation results under the same conditions, in direction, magnitude, and shape. This is the first time that E-FISH is applied to streamers of this size (>0.5 cm radius), crossing a large gap. Achieving these high resolution electric field measurements benefits further understanding of streamer discharges and enables future use of E-FISH on cylindrically symmetric (transient) electric field distributions.

Published by the American Physical Society
2025
Hotel digital intelligence capability: dimension exploration and scale development
Authors: Dong Yuelei , Wang Meng 
Published: January 2025

Purpose
This study aims to explore the dimensional structure of hotel digital intelligence capability and develop a measurement scale.

Design/methodology/approach
This study adopts qualitative and quantitative approaches to conduct an exploratory inquiry into the structural dimensions of hotel digital intelligence capability with the help of grounded theory. Based on this, several questionnaires were developed to test the measurement scale and verify its validity.

Findings
The results reveal that hotel digital intelligence capability comprises four dimensions: data collection and processing capability, customer service personalization capability, digital intelligence decision support capability and sustainable development capability. The measurement scale consists of four factors and 13 items, with reliability and validity tests demonstrating ideal levels.

Originality/value
This study not only provides a new perspective to understand hotel digital intelligence capability but also develops a corresponding measurement scale, laying a solid theoretical basis for hotel managers to scientifically evaluate this capability to achieve sustainable competitive advantage.
Prediction of pipe-jacking forces using a physics-constrained neural network
Authors: Rayner-Philipson Malachi , Sheil Brian , Zhang Pin 
Published: January 2025

Purpose
A common design driver for pipe-jacking projects is the jacking force required to advance the tunnel boring machine and pipe string. Empirical methods are popular in industry but are well known to lack accuracy, while there is a strong desire to supplement such approaches with robust data-driven techniques, typically small construction datasets present significant challenges.

Design/methodology/approach
To address this challenge, this paper develops a physics-constrained neural network predictive model for pipe-jacking forces. Information used as input into the model includes principal design information and soil type.

Findings
The physics constrained model was found to predict jacking force to a higher accuracy than current industry practice and better discern meaningful patterns in data than a purely data-driven artificial neural network. The results reveal promising performance for this initial dataset such that there is motivation, as a longer-term objective, to train the present approach on a more comprehensive drive database for more reliable and cost effective solutions for new projects.

Originality/value
Novel contributions include (a) a bespoke framework to constrain a neural network using a pipe-jacking mechanistic model which includes stoppage-induced friction increases, (b) built-in model uncertainty for greater confidence in model outputs, (c) new historical drive data for model training and (d) one-hot encoding of soil type as a model input. The model is calibrated and validated against 14 tunnel drives across four different sites with four distinctive ground types.
Toward learning recovery: challenges of international students in China during COVID-19 online learning experience
Authors: Castulo Nilo Jayoma 
Published: January 2025

Purpose
The COVID-19 pandemic has highlighted the vulnerability of international students’ education in our V.U.C.A (Volatility, Uncertainty, Complexity and Ambiguity) world. It has challenged higher education institutions to deliver quality and equitable education aligned with Sustainable Development Goal no. 4. However, studies indicate that this pandemic has significantly impacted international students in terms of both policy implementation and academic experiences in online learning. Therefore, this study aims to explore the lessons learned from the online learning experience of international students studying in China during the COVID-19 pandemic.

Design/methodology/approach
This study employed an online survey to explore international students’ experiences with online learning during the COVID-19 pandemic. Using Microsoft Forms, it collected 125 responses from international students studying at Project 211 universities. The survey included responses from 67 male and 58 female international students. Among these, 80 students studied online from locations outside China, while 45 participated in online learning from within China.

Findings
The study revealed that international students in China faced significant challenges during online learning, including academic disruptions, technological barriers, financial strain and psychological stress, exacerbated by time zone differences and isolation. Despite these obstacles, students demonstrated learning resilience by employing strategies such as self-regulation, peer collaboration and skill development in technology use.

Originality/value
This study addresses the existing gap in proper support for international students, particularly during times of crisis. It highlights lessons learned from the pandemic and presents mitigation strategies based on the participants’ perspectives. These strategies aim to address the learning poverty identified by the World Bank.
How the covid pandemic has impacted the Hong Kong sports ecosystem
Authors: Li Tak-Nang 
Published: January 2025

Purpose
This paper aims to look into how the sports ecosystem in Hong Kong was affected by the COVID-19 pandemic as the case of Hong Kong is unique from many other developed cities. Parallel to the attack of the pandemic, the period between 2019 and 2022 has also seen a major social unrest and an abrupt change of political landscape that could have unneglectable influence on the long-term development of sports in the city.

Design/methodology/approach
In view of the limitation in data and material that are available shortly after the period of study, the paper compares regular government statistics before and during the period to reflect the notable changes that were brought about by the anti-COVID measures and subsequently the demographic change resulting from the mixed impact of the global pandemic and local politics. Views of relevant stakeholders were also sought to formulate and verify the observations and conclusions of the paper.

Findings
This paper finds the sports ecosystem of Hong Kong recovered quickly and effectively from the hit of the COVID pandemic and reveals at the same time that the demographic change incurred mainly by the social unrest and its aftermath could have a longer impact on the city’s sports development.

Originality/value
This paper is one of the early works to study sports development in Hong Kong from a social-political perspective.
Improving quality of support and staff culture in a supported living service: a practice example of tier 1 positive behaviour support
Authors: Hardiman Rebecca Lyndsey , Harding Cathy 
Published: January 2025

Purpose
This paper aims to describe the approach taken to identify areas for development and to enhance positive practice, in line with a preventative tier 1 positive behaviour support (PBS) approach. The work was conducted within a supported living service, where 10 individuals with a learning disability reside.

Design/methodology/approach
The intervention was provided through a dedicated behavioural clinical specialist role over nine months. An initial assessment included measures evaluating PBS, active support and staff culture. Narrative themes were also noted from discussions with staff to deepen the understanding of informal staff culture. Together, this informed a programme of work guided by the setting of both individual and service-wide goals. Input included practice leadership, staff training, review of PBS plans and delivery of case workshops. Assessments were then repeated to evaluate the work completed.

Findings
There were indications of improvements in staff culture and practice following the intervention.

Originality/value
This practice example may be useful for other organisations to consider when thinking about how to use resources effectively to improve people’s quality of life and implement tier 1 of PBS provision.
Characteristics of suitable cases for treatment with nemolizumab based on clinical findings and cutaneous adverse events
Authors: Sugiyama Akiko , Yano‐Takamori Atsuko , Kojima Kaoru , Nishie Haruko , Nishimura Miku , Hiramoto Tetsuya , Nakahara Takeshi 
Published: January 2025

AbstractNemolizumab is an effective treatment for pruritus in atopic dermatitis, but it has a relatively high incidence of cutaneous adverse events (cAEs). To optimize the use of nemolizumab, we investigated the relationship between baseline severity in specific body areas and the frequency of cAEs. Our findings revealed that cases who discontinued treatment with nemolizumab had more severe erythema and edema/papulation on the trunk than those who continued nemolizumab. We also found that the score of excoriation on the trunk tended to be higher. These results indicate that patients with mild severity of eruptions on the trunk at initiation could be suitable for nemolizumab therapy, with the potential for long‐term continuation with the control of cAEs.
Intrauterine Contraction Monitoring in Chorioamnionitis: A Secondary, Blinded Analysis of a Randomised Controlled Trial Cohort
Authors: Juhantalo Milla , Hautakangas Tuija , Palomäki Outi , Uotila Jukka 
Published: January 2025

ABSTRACTObjectiveTo study the effects of chorioamnionitis on uterine activity and to investigate associations between external and intrauterine monitoring methods and neonatal outcomes.DesignSecondary analysis of a randomised controlled trial cohort.SettingTwo labour wards, in a university tertiary hospital and a central hospital.PopulationParturients with singleton pregnancies, gestational age ≥ 37 weeks and fetus in cephalic position. Clinical chorioamnionitis developed in 8.6% of cases (n = 130/1504).MethodsAnalyses of uterine activity (contractions/10 min or intrauterine pressure in Montevideo units) during the 4 h before birth adjusted for maternal characteristics and intrapartum factors.Main Outcome MeasuresUterine activity with or without chorioamnionitis and the intervention of external versus intrauterine monitoring in relation to fetal distress (admission to neonatal intensive care with umbilical artery pH ≤ 7.10 or 5‐min Apgar score < 7), stratified by chorioamnionitis status.ResultsUterine contraction frequency/10 min decreased in labours with chorioamnionitis during the final hour preceding birth (4.2 [SD 1.1] vs. 3.9 [SD 1.2], adjusted regression coefficient −0.383, 95% CI −0.654 to −0.112, p = 0.006) compared to non‐infected labors. In chorioamnionitis cases, mean frequency/10 min during last 4 h was higher with external versus intrauterine monitoring (4.2 [SD 0.8] vs. 3.8 [SD 0.9], MD 0.39, 95% CI 0.02–0.76, p = 0.038), and the intrauterine monitoring group had a lower incidence of fetal distress (6.7% vs. 23.1%, OR 0.24, 95% CI 0.07–0.79, p = 0.013).ConclusionsChorioamnionitis impairs uterine activity. Intrauterine monitoring may provide more accurate contraction analysis than external tocodynamometry and help reduce fetal compromise during labours complicated by chorioamnionitis.
The more things change, the more they stay the same: A multi‐wave national assessment of police academy training curricula
Authors: Sloan John J. , Paoline Eugene A. , Nobles Matt R. 
Published: January 2025

AbstractResearch SummaryUsing secondary data from a census of 421 police academies nationwide continuously operating between 2002 and 2018, we assessed continuity and change in core areas of basic law enforcement training (BLET) of new police hires. Despite decades of concerns expressed by national‐level commissions, scholars, and practitioners about the substance of police academy basic training, ours is the first study since the 1980s that examines the content of basic training at multiple police academies during an extended period that included two eras of policing: community oriented and evidence based. Results showed continuity throughout time in total required hours of BLET, including required hours of core curriculum training, and a disproportionate distribution of total training hours allotted to areas central to the crime fighter persona of the early 20th century professional era.Policy ImplicationsThis assessment revealed strong empirical evidence of long‐term resistance to change in police basic training based on continuing overemphasis of traditional aspects of basic training (e.g., use of lethal weapons) over other parts (e.g., community‐oriented policing). Our results, combined with recent qualitative analyses of police basic training revealing a primary “danger imperative” message broadcast to trainees by instructors along with more subtle racist and sexist messages about “bad guys” wanting to kill cops, reveal that decades of efforts to get the occupation to change what new police officers learn during basic training is apparently the equivalent of what Dorothy Guyot once termed “bending granite”—an effort doomed to fail. To weaken the granite, police practitioners and leaders involved with training must take steps toward a complete and comprehensive reorientation of police recruitment and basic training that stresses the importance of new hires acquiring a toolkit that has a guardian‐based foundation and emphasis but allows for the rare instances when officers need to use warrior tools.
Assessment of Exhaled Breath Condensate for ALK, RET, ROS1, and NTRK1 Fusion Transcript Detection in NSCLC: Comparison With Tissue and Liquid Biopsy Samples
Authors: Tetik Vardarlı Aslı , Karakus Haydar Soydaner , Korba Korcan , Boluk Burcu , Ozgur Su , Gunduz Cumhur , Pelit Fusun , Veral Ali , Goksel Tuncay 
Published: January 2025

ABSTRACTBackgroundLung cancer continues to be the primary cause of cancer‐related deaths globally, with the majority of cases identified at advanced stages. Genetic alterations, including mutations and gene fusions, are central to its molecular pathogenesis. The discovery of therapeutically targetable gene fusions, such as ALK, RET, ROS1, and NTRK1, has significantly advanced lung cancer management. Conventional methods, such as tissue biopsies, are invasive and unsuitable for continuous molecular monitoring. Consequently, noninvasive approaches, such as liquid biopsies and exhaled breath condensate (EBC), offer promising options for real‐time molecular surveillance.MethodsThis study evaluates the feasibility of identifying fusion transcripts in 30 patients with lung adenocarcinoma by using next‐generation sequencing (NGS) on formalin‐fixed paraffin‐embedded (FFPE) tissue, plasma, and EBC samples.ResultsClinically significant fusion transcripts, including KIF5B‐ALK, KIF5B‐RET, and SQSTM1‐ALK, were detected across different sample types. EBC samples showed strong concordance with tissue biopsy results, particularly in detecting ALK, ROS1, and RET fusions, and demonstrated greater sensitivity than plasma in detecting NTRK1 fusions. Additionally, 30 fusion transcripts of uncertain clinical significance were identified, highlighting the need for further research into their role in lung cancer pathogenesis.ConclusionIn conclusion, EBC samples provide a valuable, noninvasive medium for detecting clinically relevant and previously uncharacterized fusion transcripts in non‐small cell lung cancer (NSCLC). The high concordance between EBC and tissue biopsies suggests that EBC could complement tissue biopsy for effective diagnosis and monitoring of NSCLC. These findings underscore the importance of comprehensive molecular profiling using multiple sample types to enhance diagnostic precision and optimize therapeutic outcomes in lung cancer management.
“Accounting” in retrospect: A personal reflection about change, contribution, and opportunity following decades of immersion
Authors: Deegan Craig 
Published: January 2025

AbstractThis paper, based upon my Plenary Address at the 2024 Accounting and Finance Association of Australia and New Zealand Annual Conference, documents my reflection of the evolution in accounting that has occurred over the last 40 years. While the research and teaching of accounting has evolved, many gaps are evident. Applying a four‐step accountability model, opportunities for future research and teaching are identified. Factors influencing research and teaching agendas, and our propensity to address gaps, are explored. This paper is a useful resource for teachers and researchers of accounting, students and people seeking some tips about potential accounting research topics.