bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of circular RNAs in bovine mammary epithelial cells induced by Escherichia coli LPS
Authors: LIANG YAN , Xia Yuxin , Wang Mengqi , Li Mingxun , Yang Zhangping , Karrow Niel A. , Mao Yongjiang 
Published: May 2023

Abstract

The rise of multi-omics technology in recent years provides convenient for in-depth study of the pathogenesis of dairy cow mastitis, and circRNAs, as endogenous non-coding RNAs, are expected to become molecular targets to study the pathogenesis of dairy cow mastitis. LPS, as a component of the outer wall of E. coli cell wall, is a common endotoxin in the construction of inflammatory models. The objective of this study is to identified and compared circular RNAs (circRNAs) from bovine mammary epithelial cells (bMECs) between the control and LPS groups. The expression profiles of circRNAs were obtained by high-throughput sequencing (RNA-seq) based on the construction of bMECs - LPS inflammation model, with control group (n = 3) and LPS group (n = 3) cell RNA as samples. After analysis, we identified 4323 circRNAs, ranging from 63 bp to 96387 bp. Chromosome 5 had most circRNAs, containing 259 circRNAs. Furthermore, 87.42% of the circRNAs belonged to sense-overlapping circRNA. CircRNAs contains different number of exons, ranging from 1 to 43, and most of cirsRNAs harbored 1 to 5 exons. Compared with the negative control (NC) group, 841 circRNAs with significantly different expressions (DE) in the LPS group (10 μg/mL), including 400 upregulated and 441 downregulated circRNAs. Enrichment analysis revealed the enrichment of circRNAs in methylation, such as positive regulation of G1/S transition of the mitotic cell cycle, histone methyltransferase activity (H3-K27 specific), and DNA methylation. The significantly enriched pathways further indicate that circRNAs play important roles in immunoreaction, such as hippo signaling pathway – fly, AMPK signaling pathway, and Fc gamma R-mediated phagocytosis. This study revealed the expression profile and characteristics of circRNAs in bMECs induced by LPS, and providing information for studying circRNA functions and mechanisms underlying mastitis, which suggesting a new avenue to investigate the regulatory mechanisms of mastitis.
The comprehensive expression of BCL2 family genes determines the prognosis of diffuse large B-cell lymphoma]{The comprehensive expression of BCL2 family genes determines the prognosis of diffuse large B-cell lymphoma
Authors: Roh Jin , Pak Hyo-Kyung , Jeong Seongfeel , Hwang Sewon , Kim Do Eon , Choi Hwal-Seok , Kim So-Jeong , Kim Hyunji , Cho Hyungwoo , Park Joon Seong , Jeong Seong Hyun , Choi Yoon Seok , Han Jae Ho , Yoon Dok Hyun , Park Chan-Sik 
Published: May 2023

Abstract
Diffuse large B-cell lymphoma (DLBCL) is a prevalent and aggressive non-Hodgkin’s lymphoma, and 40% of patients succumb to death. Despite numerousclinical trials aimed at developing treatment strategies beyond the conven-tional R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, andprednisone) regimen, there have been no positive results thus far. Although theselective BCL2 inhibitor venetoclax has shown remarkable efficacy in chronic lym-phocytic leukemia, its therapeutic effect in DLBCL was limited. We hypothesizedthat the limited therapeutic effect of venetoclax in DLBCL may be attributedto the complex expression and interactions of BCL2 family members, includingBCL2. Therefore, we aimed to comprehensively analyze the expression patternsof BCL2 family members in DLBCL. We analyzed 157 patients with de novoDLBCL diagnosed at Asan Medical Center and Ajou University Hospital. ThemRNA expression levels of BCL2 family members were quantified using theNanoString technology. BCL2 family members showed distinct heterogeneousexpression patterns both intra- and inter-patient. Using unsupervised hierarchi-cal cluster analysis, we were able to classify patients with similar BCL2 familyexpression pattern and select groups with clear prognostic features, C1 and C6.In the group with the best prognosis, C1, the expression of pro-apoptotic and pro-apoptotic BH3-only group gene expressions were increased, while anti-apoptoticgroup expression was significantly increased in both C1 and C6. Based on this, wegenerated the BCL2 signature score using the expression of pro-apoptotic genesBOK and BCL2L15, and anti-apoptotic gene BCL2. The BCL2 signature score0 had the best prognosis, score 1/2 had intermediate prognosis, and score 3 hadthe worst prognosis (EFS, p = 0.0054; OS, p = 0.0011). Multivariate analysis,including COO and IPI, showed that increase in the BCL2 signature score wassignificantly associated with poor prognosis for EFS, independent of COO andIPI. The BCL2 signature score we proposed in this study provides information onBCL2 family deregulation based on the equilibrium of pro- versus anti-apoptoticBCL2 family, which can aid in the development of new treatment strategies forDLBCL in the future.
Interactions between isolated pea globulins and purified egg white proteins in solution
Authors: Kuang Jian , Hamon Pascaline , Rousseau Florence , Cases Eliane , Bouhallab Saïd , Saurel Rémi , Lechevalier Valérie 
Published: May 2023

Abstract
In the present work, the interactions and associations between low denatured pea globulins (PPI) and purified main egg white proteins (ovalbumin (OVA), ovotransferrin (OVT), and lysozyme (LYS)) were studied at pH 7.5 and 9.0 by using isothermal titration calorimetry (ITC), dynamic light scattering (DLS), laser granulometry and confocal laser scanning microscopy (CLSM). From ITC, we detected strong exothermic interactions between PPI and LYS at both pHs, which led to aggregation. At these pH values, the net positive charge of lysozyme favored electrostatic interactions with negative charges of pea proteins, and oligomers were formed during titration experiments. Furthermore, DLS, laser granulometry, and CLSM data showed that the particle size of the mixture increased with increasing LYS to PPI molar ratio (from 0.8 to 20). Large irregular aggregates up to 20–25 µm were formed at high molar ratios and no complex coacervate was observed. No or very weak interactions were detected between OVT or OVA and PPI whatever the pH. These results suggest the role of electrostatic interactions between LYS and PPI when considering protein mixtures.
A Psychometric Investigation of Inter-Professional Education Competency Tool among the Students of Medicine and Nursery
Authors: Rasouli Davood , Norouzi Azam , Ramezani Ghobad , Akram Hashemi 
Published: May 2023

Abstract
Introduction
Collaboration between nurses and doctors is necessary for offering care to patients. Using team performance assessment tools and surveying them can be effective in promoting inter-professional collaboration, and the lack of a credible tool to assess inter-professional collaboration competency between the two groups is a major challenge in the healthcare sector. The present study aimed to translate and conduct a psychometric investigation on the inter-professional education collaboration (IPEC) tool for the students of medicine and nursery.
Methods
The present study was a cross-sectional one conducted as a psychometric investigation of the IPEC tool at the Iran University of Medical Sciences in 2022. The initial tool contained 42 items developed according to a 5-point Likert scale, which was translated into Persian with the consent of the original researcher. The validity index and the content validity ratio were investigated by a panel of 11 specialists in medical and clinical education, and its construct validity was evaluated using confirmatory factor analysis. Moreover, the reliability of the instrument was investigated using internal consistency, Cronbach’s Alpha, and test-retest methods.
Results
based on the indicators calculated to perform a psychometric investigation over the above tool, it had acceptable reliability and validity according to the specialists. The tool evaluates inter-professional collaboration competency between the students of medicine and nursery across four areas (values and ethics, roles and responsibilities, inter-professional communication, and teams and teamwork). Moreover, Cronbach’s Alpha coefficient for the tool was determined at 0.84.
Conclusion
The results of the study showed that the above tool could evaluate inter-professional competency as a valid and reliable questionnaire, and its results could be utilized in planning and education.
Long-term fertilizer regimes influences on weed floristic diversity under Typic Hapludalfs soil conditions
Authors: Kumar Pawan , Kumar Sachin , Rana Surinder Singh , Sharma Raj Paul , Sankhyan NK 
Published: May 2023

Abstract
We investigated the effect of different sources of organic and inorganic nutrients on weed composition, diversity and system productivity in maize-wheat cropping system in long-term experiment initiated during 1972-73 in a Typic Hapludalfs soil conditions of North-Western Himalaya. The experiment was carried out in a randomized block design with 11 treatments replicated three times. Results revealed that a total of 9 weed species from 3 families and 11 weed species from 7 families were recorded in maize and wheat crops, respectively. Poaceae was the most dominating family followed by Asteraceae in wheat crop. 50% NPK resulted in significantly higher broadleaf weed count, total weed count and total weed dry weight, whereas, 100% NPK + Zn resulted in the maximum count of grasses in maize crop. In wheat crop, broadleaf count, total weed count and total weed dry weight were significantly higher in 150% NPK, whereas, 100% NPK + FYM had maximum grassy weeds population. Balanced fertilization resulted in significantly lower species richness, Shannon’s equitability and diversity indices and higher system productivity of maize-wheat cropping system.
Horses discriminate between human facial and vocal expressions of sadness and joy
Authors: Jardat Plotine , Liehrmann Océane , Reigner Fabrice , Parias Céline , Calandreau Ludovic , Lansade Léa 
Published: May 2023

AbstractCommunication of emotions plays a key role in intraspecific social interactions and likely in interspecific interactions. Several studies have shown that animals perceive human joy and anger, but few studies have examined other human emotions, such as sadness. In this study, we conducted a cross-modal experiment, in which we showed 28 horses two simultaneous soundless videos featuring a sad and a joyful face, which were accompanied by either a sad or joyful voice. The number of horses who initially looked more at the video that was incongruent with the voice was higher than chance, suggesting that horses could form cross-modal representations of human joy and sadness. Moreover, horses were more attentive to the videos of joy and looked at them for longer, more frequently, and more rapidly than the videos of sadness. Their heart rates tended to increase when they heard joy and to decrease when they heard sadness. These results show that horses are able to discriminate facial and vocal expressions of joy and sadness and may form cross-modal representations of these emotions; they also are more attracted to joyful faces than to sad faces and seem to be more aroused by a joyful voice than a sad voice. Further studies are needed to better understand how horses perceive the range of human emotions, and we propose that future experiments include neutral stimuli as well as emotions with different arousal levels but a same valence.
Blockchain-based Multi-Diagnosis Deep Learning Application for Various Diseases Classification
Authors: Rahal Hakima Rym , Slatnia Sihem , Kazar Okba , Barka Ezedin , Harous Saad 
Published: May 2023

Abstract
Misdiagnosis is a critical issue in healthcare, which can lead to severe consequences for patients, in- cluding delayed or inappropriate treatment, unneces- sary procedures, psychological distress, financial bur- den, and legal implications. To mitigate this issue, we propose using deep learning algorithms to improve diag- nostic accuracy. However, building accurate deep learn- ing models for medical diagnosis requires substantial amounts of high-quality data, which can be challeng- ing for individual healthcare sectors or organizations to acquire. Therefore, combining data from multiple sources to create a diverse dataset for efficient train- ing is needed. However, sharing medical data between different healthcare sectors can be problematic from a security standpoint due to sensitive information and privacy laws. To address these challenges, we propose using Blockchain technology to provide a secure, de- centralized, and privacy-respecting way to share locally trained deep learning models instead of the data itself. Our proposed method of model ensembling, which com- bines the weights of several local deep learning models to build a single global model, that enables accurate diagnosis of complex medical conditions across multi- ple locations while preserving patient privacy and data security. Our research demonstrates the effectiveness of this approach in accurately diagnosing three diseases (Breast cancer, Lung cancer, and Diabetes) with high accuracy rates, surpassing the accuracy of local models and building a multi-diagnosis application.
How can we live within the safe and just Earth system boundaries for blue water?
Authors: Stewart-Koster Ben , Bunn Stuart , Green Pamela , Ndehedehe Christopher , Andersen Lauren , McKay David Armstrong , Bai Xuemei , DeClerck Fabrice , Ebi Kristie , Gordon Christopher , Gupta Joyeeta , Hasan Syezlin , Jacobson Lisa , Lade Steven , Liverman Diana , Mohamed Awaz , Loriani Sina , Nakicenovic Nebojsa , Obura David , Qin Dahe , Rammelt Crelis , Rocha Juan , Rockström Johan , Verburg Peter , Zimm Caroline 
Published: May 2023

Abstract

Safe and just Earth System Boundaries (ESBs) for surface and groundwater (blue water) have been defined for sustainable water management in the Anthropocene. We evaluate where minimum human needs can be met within the surface water ESB and, where this is not possible, identify how much groundwater is required. 2.6 billion people live in catchments where groundwater is needed because they are already outside the surface water ESB or have insufficient surface water to meet human needs and the ESB. Approximately 1.4 billion people live in catchments where demand side transformations are required as they either exceed the surface water ESB or face a decline in groundwater recharge and cannot meet minimum needs within the ESB. A further 1.5 billion people live in catchments outside the ESB with insufficient surface water to meet needs, requiring both supply and demand-side transformations. These results highlight the challenges and opportunities of meeting even basic human access needs to water and protecting aquatic ecosystems.
Correlates of poor oral health related quality of life in a cohort of people who use methamphetamine in Australia
Authors: Abdelsalam Shady , Livingston Michael , Quinn Brendan , Agius Paul A , Ward Bernadette , Jamieson Lisa , Dietze Paul 
Published: May 2023

Abstract
Objectives Methamphetamine use impacts oral health, but little is known about its impacts on oral health related quality of life (OHRQoL). In this study we examined OHRQoL in a cohort of people who use methamphetamine and assessed associations with sociodemographic, behavioural, psychosocial and dental service utilisation correlates. A secondary aim was to examine the relationship between methamphetamine route of administration and OHRQoL, to test whether smoking the drug is associated with reduced OHRQoL.Methods Cross-sectional analysis was performed, using data from VMAX, a cohort of people who use methamphetamine at least monthly in Victoria, Australia (n = 194). Utilising the oral health impact profile (OHIP-14), we assessed three OHRQoL outcomes: OHIP-14 prevalence, OHIP-14 extent and OHIP-14 severity. Regression analyses examined associations between independent variables and the three OHIP-14 outcome measures.Results A significant segment of the cohort (35%) reported poor OHRQoL. Overall, no statistically significant association was detected between methamphetamine route of administration and the three OHIP-14 outcomes. Participants living in rural areas, with moderate to severe self-reported depression and with methamphetamine dependence had significantly worse OHRQoL levels, which persisted after adjusting for other covariates.Conclusion Overall, VMAX cohort participants reported reduced OHRQoL levels. Our findings highlight the need for upstream interventions to improve the OHRQoL of people who use methamphetamine, with specific focus on those living in rural locations. Further research on the links between OHRQoL and mental health among people who use methamphetamine is required.
A Proposal for Experimentally Testing Different “Interpretations” of Quantum Mechanics
Authors: Qadir Asghar 
Published: May 2023

Abstract
It used to be assumed that the differences between “interpretations” of quantummechanics are philosophical and not physical. The experimental test of the original EPR “thought experiment” by Aspect et al., using the Bell inequalities, eliminatedthe Bohm-Aharanov local hidden variables, demonstrating that the so-called interpretations are distinct theories, amenable to experimental testing, and not merely philosophically different “interpretations”. In this paper I present a sequence of experiments, doable by current technology, to distinguish between various “interpretations” of Quantum Mechanics.
Evaluation of the Cardiac Functions With Tissue Doppler Before and After Hyperbaric Oxygen Therapy of Patients Withcarbonmonoxide Poisoning
Authors: Simsek Ahmet Burak , Gursu Hazım Alper , Azak Emine , Sahan Yasemin Ozdemir , Gul Ayse Esin Kibar , Kurt Funda , Cetin Ibrahim Ilker 
Published: May 2023

Abstract
Objective: We aimed to determine whether systolic and diastolic functions of patients who presented to the pediatric emergency clinic because of carbonmonoxide poisoning and were treated with hyperbaric oxygen therapy, were examined with tissue doppler imaging method before and after the treatment.
Materials and Methods: Between January 2019-January 2021, 34 patients who presented to the pediatric emergency clinic because of carbonmonoxide poisoning and were treated with hyperbaric oxygen, and 34 healthy children who presented to the pediatric cardiology clinic as the control group were included. The study was performed prospectively.
Results: The mean age of patients was 10.95 ± 4.75 years. 21 patients (61.8%) were female. 21 patients (61.8%) presented with natural gas poisoning. The most common complaint at admission to the hospital was loss of consciousness with 41.2%. The mean carboxyhemoglobin of the patients after carbonmonoxide poisoning was found to be 21.70±6.45. After one session of hyperbaric oxygen therapy, the mean carboxyhemoglobin was found to be 0.74±0.34. The mean troponin-I level of the patients was 114.81±197.76 after carbonmonoxide poisoning and 42.32±118.36 after one session of hyperbaric oxygen therapy. In the tissue doppler imaging of the patients, the isovolemic relaxation time and myocardial performance index were higher in the group evaluated before hyperbaric oxygen therapy; ejection time was found to be shorter.
Conclusions: Systolic and diastolic dysfunctions were found in the cardiac evaluation of patients who presented with carbonmonoxide poisoning and were treated with hyperbaric oxygen. It has been demonstrated echocardiographically that this dysfunction is reversible with hyperbaric oxygen therapy.
Classification and Recognition of Ferroresonance Based on Improved Elman-Adaboost
Authors: Kang Zhongjian , Li Longchen 
Published: May 2023

Abstract
Aiming at the problem that the fault suppression effect is not obvious due to the unclear type of ferroresonance, an improved Elman-Adaboost method for classification and identification of ferroresonance was proposed. Firstly, the characteristic values of different types of ferroresonance overvoltage were extracted, and then a classifier was added to the traditional Elman-Adaboost model to automatically find the optimal hidden layer, and the corresponding weighted parameters were added and adjusted when the training set was recognized correctly. Finally, by constructing a two-stage improved Elman-Adaboost model to test different types of ferroresonance samples and comparing with other methods, the accuracy of the proposed method is verified. The results show that the proposed method can effectively identify the type of ferroresonance in power systems, and the identification accuracy is greatly improved. It has potential application value in the identification and suppression of ferroresonance faults.
Working Less for Longer: Unintended Effects of Longevity Adjustment of Retirement Age
Authors: Jensen Svend E. Hougaard , Sveinsson Thorsteinn Sigurdur , Vieira Filipe Bonito 
Published: May 2023

Abstract

This paper utilises a model with overlapping generations ad modum Blanchard-Yaari and shows that by broadening the labour supply on the extensive margin through longevity adjustment of the statutory retirement age, labour supply is likely to decrease on the intensive margin. This backlash effect depends strongly on the specific design of the pension system: It is significantly higher under a pay-as-you-go scheme with fixed benefits compared to a pay-as-you-go scheme with fixed contributions and a fully funded scheme based on voluntary savings. The findings have implications for how to address the fiscal and macroeconomic challenges raised by an ageing population.
JEL codes: E21, J21, J26, H55
Efficacy of Careolizumab combined with chemotherapy and astragalus polysaccharide(APS) in patients with intermediate to advanced NSCLC and effects on lymphocyte subsets, regulatory T cells and serum tumor markers
Authors: Dong Wang , xin Wan 
Published: May 2023

Abstract

Objctive:To observe the recent efficacy of the combination group on patients with intermediate to advanced non-small cell lung cancer (NSCLC) and the effects on lymphocyte subsets, regulatory T cells, serum cytokeratin 19 fragment (CYFRA21-1), angiopoietin-2 (Ang-2), and vascular endothelial growth factor (VEGF). Methods:188 patients with intermediate to advanced NSCLC admitted to our hospital from August 2021 to April 2023 were selected, and the patients were simply randomly divided into a combination group and a control group. The control group was treated with chemotherapy regimen, and the combination group was treated with Carelizumab and astragalus polysaccharide on top of it. The recent efficacy, lymphocyte subsets (B, NK, CD3+,CD4+, CD4+/CD8+), regulatory T cells (CD4+Treg) , serum tumor markers CYFRA21-1, carcinoembryonic antigen (CEA), tumor antigen 125 (CA125), neurospecific enolase (NSE)] and angiogenic indexes (Ang-2, VEGF). Results:The total effective rate of recent clinical efficacy in the combined group after treatment was 69.15%, which was significantly higher than that in the control group (38.3%) (P<0.05). After treatment, the levels of B, NK, CD3+, CD4+ and CD4+/CD8+ were reduced in both groups, and the levels in the combined group were higher than those in the control group (P<0.05); the levels of CYFRA21-1, CEA, CA125 and NSE were reduced in both groups, and the levels in the combined group were significantly lower than those in the control group (P<0.05); the levels of Ang-2 and The levels of Ang-2 and VEGF were reduced in both groups, and the levels of Ang-2 and VEGF in the combined group were significantly lower than those in the control group (P<0.05). Progression-free survival was higher in the combination group than in the control group (95% CI, 1.468-4.278; Log-rank P < 0.0001; HR, 2.506). Conclusion: Carelizumab combined with chemotherapy and astragalus polysaccharide has definite near-term efficacy in patients with intermediate to advanced NSCLC, and can reduce the impact on immune function, prolong patients' progression-free survival, significantly improve serum tumor marker levels and play a certain inhibitory effect on angiogenesis.