bluuebunny/crossref_metadata_2025_split huggingface dataset:
One‐Parameter Analytical Derivation in Modified Budyko Framework for Unsteady‐State Streamflow Elasticity in Humid Catchments
Authors: Han Peng‐Fei , Sankarasubramanian Arumugam , Wang Xu‐Sheng , Wan Li , Yao Lili 
Published: August 2023

AbstractThe changes in climate and catchment properties have altered the hydrological processes significantly at different spatiotemporal scales around the world. In particular for finer time scales, changes in water storage, which has been commonly neglected for long‐term temporal scales, may play an important role on hydrological processes. Nevertheless, few studies addressed this question in modifying the Budyko framework, with most of them remaining a steady‐state assumption on catchment characteristics. Here, we derive new analytical formulas of unsteady‐state streamflow elasticity in a modified Budyko framework, incorporating both storage change and one specific parameter for catchment properties. We study 78 humid catchments in the USA with simulation data of daily hydrological processes from a probability‐distribution‐based hydrological model (the modified HyMOD). As indicated by results, the annual storage change ratio is linearly correlated with the annual aridity index, and this relationship can be used to estimate elasticity coefficients with our formulas. The estimated elasticity coefficients perform well in simulating the annual streamflow with the power‐law model. For different catchments, variability of the unsteady‐state elasticity is higher than that of the steady‐state elasticity. Unsteady‐state streamflow coefficients show significant linear correlation with catchment properties, such as the average slope, average elevation, and catchment area. This study provides a new analytical approach to investigate the interannual stability of catchments with varying climate and catchment properties.
Atp6i deficient mouse model uncovers transforming growth factor-β1 /Smad2/3 as a key signaling pathway regulating odontoblast differentiation and tooth root formation
Authors: Wang Jue , McVicar Abigail , Chen Yilin , Deng Hong-Wen , Zhao Zhihe , Chen Wei , Li Yi-Ping 
Published: August 2023

AbstractThe biomolecular mechanisms that regulate tooth root development and odontoblast differentiation are poorly understood. We found that Atp6i deficient mice (Atp6i−/−) arrested tooth root formation, indicated by truncated Hertwig’s epithelial root sheath (HERS) progression. Furthermore, Atp6i deficiency significantly reduced the proliferation and differentiation of radicular odontogenic cells responsible for root formation. Atp6i−/− mice had largely decreased expression of odontoblast differentiation marker gene expression profiles (Col1a1, Nfic, Dspp, and Osx) in the alveolar bone. Atp6i−/− mice sample RNA-seq analysis results showed decreased expression levels of odontoblast markers. Additionally, there was a significant reduction in Smad2/3 activation, inhibiting transforming growth factor-β (TGF-β) signaling in Atp6i−/− odontoblasts. Through treating pulp precursor cells with Atp6i−/− or wild-type OC bone resorption-conditioned medium, we found the latter medium to promote odontoblast differentiation, as shown by increased odontoblast differentiation marker genes expression (Nfic, Dspp, Osx, and Runx2). This increased expression was significantly blocked by anti-TGF-β1 antibody neutralization, whereas odontoblast differentiation and Smad2/3 activation were significantly attenuated by Atp6i−/− OC conditioned medium. Importantly, ectopic TGF-β1 partially rescued root development and root dentin deposition of Atp6i−/− mice tooth germs were transplanted under mouse kidney capsules. Collectively, our novel data shows that the prevention of TGF-β1 release from the alveolar bone matrix due to OC dysfunction may lead to osteopetrosis-associated root formation via impaired radicular odontoblast differentiation. As such, this study uncovers TGF-β1 /Smad2/3 as a key signaling pathway regulating odontoblast differentiation and tooth root formation and may contribute to future therapeutic approaches to tooth root regeneration.
A three-colour stress biosensor reveals multimodal response in single cells and spatiotemporal dynamics of biofilms
Authors: Zoheir Ahmed E. , Sobol Morgan S. , Meisch Laura , Ordoñez-Rueda Diana , Kaster Anne-Kristin , Niemeyer Christof M. , Rabe Kersten S. 
Published: August 2023

AbstractThe plethora of stress factors that can damage microbial cells has evolved sophisticated stress response mechanisms. While existing bioreporters can monitor individual responses, sensors for detecting multimodal stress responses in living microorganisms are still lacking. Orthogonally detectable red, green, and blue fluorescent proteins combined in a single plasmid, dubbed RGB-S reporter, enable simultaneous, independent, and real-time analysis of the transcriptional response of Escherichia coli using three promoters which report physiological stress (PosmY for RpoS), genotoxicity (PsulA for SOS), and cytotoxicity (PgrpE for RpoH). The bioreporter is compatible with standard analysis and Fluorescent Activated Cell Sorting (FACS) combined with subsequent transcriptome analysis. Various stressors, including the biotechnologically relevant 2-propanol, activate one, two, or all three stress responses, which can significantly impact non-stress-related metabolic pathways. Implemented in microfluidic cultivation with confocal fluorescence microscopy imaging, the RGB-S reporter enabled spatiotemporal analysis of live biofilms revealing stratified subpopulations of bacteria with heterogeneous stress responses.
Enhancing the Faradaic efficiency of solid oxide electrolysis cells: progress and perspective
Authors: Gaikwad Prashik S. , Mondal Kunal , Shin Yun Kyung , van Duin Adri C. T. , Pawar Gorakh 
Published: August 2023

AbstractTo reduce global warming, many countries are shifting to sustainable energy production systems. Solid oxide electrolysis cells (SOECs) are being considered due to their high hydrogen generation efficiency. However, low faradaic efficiency in scaling SOEC technology affects costs and limits large-scale adoption of hydrogen as fuel. This review covers SOECs’ critical aspects: current state-of-the-art anode, cathode, and electrolyte materials, operational and materials parameters affecting faradaic efficiency, and computational modeling techniques to resolve bottlenecks affecting SOEC faradaic efficiency.
Thermodynamics of multi-sublattice battery active materials: from an extended regular solution theory to a phase-field model of LiMnyFe1-yPO4
Authors: Ombrini Pierfrancesco , Bazant Martin Z. , Wagemaker Marnix , Vasileiadis Alexandros 
Published: August 2023

AbstractPhase separation during the lithiation of redox-active materials is a critical factor affecting battery performance, including energy density, charging rates, and cycle life. Accurate physical descriptions of these materials are necessary for understanding underlying lithiation mechanisms, performance limitations, and optimizing energy storage devices. This work presents an extended regular solution model that captures mutual interactions between sublattices of multi-sublattice battery materials, typically synthesized by metal substitution. We apply the model to phospho-olivine materials and demonstrate its quantitative accuracy in predicting the composition-dependent redox shift of the plateaus of LiMnyFe1-yPO4 (LFMP), LiCoyFe1-yPO4 (LFCP), LiCoxMnyFe1-x-yPO4 (LFMCP), as well as their phase separation behavior. Furthermore, we develop a phase-field model of LFMP that consistently matches experimental data and identifies LiMn0.4Fe0.6PO4 as a superior composition that favors a solid solution phase transition, making it ideal for high-power applications.
Perspectives for plant biology in space and analogue environments
Authors: De Micco Veronica , Aronne Giovanna , Caplin Nicol , Carnero-Diaz Eugénie , Herranz Raúl , Horemans Nele , Legué Valérie , Medina F. Javier , Pereda-Loth Veronica , Schiefloe Mona , De Francesco Sara , Izzo Luigi Gennaro , Le Disquet Isabel , Kittang Jost Ann- Iren 
Published: August 2023

AbstractAdvancements in plant space biology are required for the realization of human space exploration missions, where the re-supply of resources from Earth is not feasible. Until a few decades ago, space life science was focused on the impact of the space environment on the human body. More recently, the interest in plant space biology has increased because plants are key organisms in Bioregenerative Life Support Systems (BLSS) for the regeneration of resources and fresh food production. Moreover, plants play an important role in psychological support for astronauts. The definition of cultivation requirements for the design, realization, and successful operation of BLSS must consider the effects of space factors on plants. Altered gravitational fields and radiation exposure are the main space factors inducing changes in gene expression, cell proliferation and differentiation, signalling and physiological processes with possible consequences on tissue organization and organogenesis, thus on the whole plant functioning. Interestingly, the changes at the cellular and molecular levels do not always result in organismic or developmental changes. This apparent paradox is a current research challenge. In this paper, the main findings of gravity- and radiation-related research on higher plants are summarized, highlighting the knowledge gaps that are still necessary to fill. Existing experimental facilities to simulate the effect of space factors, as well as requirements for future facilities for possible experiments to achieve fundamental biology goals are considered. Finally, the need for making synergies among disciplines and for establishing global standard operating procedures for analyses and data collection in space experiments is highlighted.
Revisiting the “forever chemicals”, PFOA and PFOS exposure in drinking water
Authors: Wee Sze Yee , Aris Ahmad Zaharin 
Published: August 2023

AbstractPerfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), known as the most detected per- and polyfluoroalkyl substances (PFAS) in various environmental compartments, have been associated with plastic pollution and endocrine dysfunction. Over the past 180 years (1839–2019), numerous emerging contaminants have been identified, with PFOA and PFOS receiving considerable attention based on scientific evidence and publications. Between 2018 and 2019, PFOA and PFOS experienced a relatively high increase rate of 18.8% and 13.6%, respectively. While developed countries have made progress in establishing stringent guidelines, developing and underdeveloped countries often lack regulations and mechanisms to address emerging PFAS. Furthermore, advancements in PFAS removal technologies are needed to improve their efficacy and feasibility. The establishment of regulatory compliances, along with exposure assessment and risk characterization, is essential for providing precautionary advice on water source protection, water supply security, health risks, treatment efficiency, and contamination forecasting. However, a more comprehensive approach and database for evaluating exposure and risks are still imperative to effectively combat PFAS contamination in drinking water. Therefore, this review aims to enhance environmental monitoring and management practices in response to the global crisis of PFAS contamination. The analysis of Needs, Approaches, Benefits, and Challenges (NABC) is grounded in the current trends of PFAS in the environment and human exposure through drinking water.
Phytochemical mediated modulation of COX-3 and NFκB for the management and treatment of arthritis
Authors: Biswas Dipak , Somkuwar Bharat Gopalrao , Borah Jagat Chandra , Varadwaj Pritish Kumar , Gupta Saurabh , Khan Zeeshan Ahmad , Mondal Gopinath , Chattoraj Asamanja , Deb Lokesh 
Published: August 2023

AbstractIn this study, we investigated whether zerumbone (ZBN), ellagic acid (ELA) and quercetin (QCT), the plant-derived components, can modulate the role of COX-3 or cytokines liable in arthritic disorder. Initially, the effect of ZBN, ELA, and QCT on inflammatory process was investigated using in-vitro models. In-silico docking and molecular dynamics study of these molecules with respective targets also corroborate with in-vitro studies. Further, the in-vivo anti-arthritic potential of these molecules in Complete Freund’s adjuvant (CFA)-induced arthritic rats was confirmed. CFA increases in TNF-α and IL-1β levels in the arthritic control animals were significantly (***p < 0.001) attenuated in the ZBN- and ELA-treated animals. CFA-induced attenuation in IL-10 levels recovered under treatment. Moreover, ELA attenuated CFA-induced upregulation of COX-3 and ZBN downregulated CFA-triggered NFκB expression in arthritic animals. The bonding patterns of zerumbone in the catalytic sites of targets provide a useful hint in designing and developing suitable derivatives that can be used as a potential drug. To our best knowledge, the first time we are reporting the role of COX-3 in the treatment of arthritic disorders which could provide a novel therapeutic approach for the treatment of inflammatory disorders.
Prevalence, incidence, and outcomes of hepatitis E virus coinfection in patients with chronic hepatitis C
Authors: Jang Eun Sun , Choi Gwang Hyeon , Kim Young Seok , Kim In Hee , Lee Youn Jae , Cho Sung Beom , Kim Yun-Tae , Jeong Sook-Hyang 
Published: August 2023

AbstractThis study aimed to elucidate the anti-hepatitis E virus (HEV) immunoglobulin G (IgG) prevalence and incidence of seroconversion and seroreversion as well as its risk factors and to analyze the clinical outcomes of HEV and hepatitis C virus (HCV) coinfected patients compared to those of HCV-monoinfected patients. We prospectively enrolled 502 viremic HCV patients with paired plasma samples (at intervals of ≥ 12 months) from 5 tertiary hospitals. Anti-HEV IgG positivity was tested using the Wantai ELISA kit in all paired samples. Mean age was 58.2 ± 11.5 years old, 48.2% were male, 29.9% of patients had liver cirrhosis, and 9.4% of patients were diagnosed with hepatocellular carcinoma (HCC). The overall prevalence of anti-HEV IgG positivity at enrollment was 33.3%, with a higher prevalence in males and increasing prevalence according to the subject’s age. During the 916.4 person-year, the HEV incidence rate was 0.98/100 person-years (9/335, 2.7%). Hepatic decompensation or liver-related mortality was not observed. There were six seroreversion cases among 172 anti-HEV-positive patients (1.22/100 person-years). In conclusion, approximately one-third of the adult Korean chronic HCV patients were anti-HEV IgG positive. The HEV incidence rate was 1 in 100 persons per year, without adverse hepatic outcomes or mortality.
Vacuolar-ATPase-mediated muscle acidification caused muscular mechanical nociceptive hypersensitivity after chronic stress in rats, which involved extracellular matrix proteoglycan and ASIC3
Authors: Nasu Teruaki , Hori Amane , Hotta Norio , Kihara Chiaki , Kubo Asako , Katanosaka Kimiaki , Suzuki Masamitsu , Mizumura Kazue 
Published: August 2023

AbstractAlthough widespread pain, such as fibromyalgia, is considered to have a central cause, peripheral input is important. We used a rat repeated cold stress (RCS) model with many characteristics common to fibromyalgia and studied the possible involvement of decreased muscle pH in muscle mechanical hyperalgesia. After a 5-day RCS, the muscle pH and the muscular mechanical withdrawal threshold (MMWT) decreased significantly. Subcutaneously injected specific inhibitor of vacuolar ATPase (V-ATPase), bafilomycin A1, reversed both changes almost completely. It also reversed the increased mechanical response of muscle thin-fibre afferents after RCS. These results show that V-ATPase activation caused muscle pH drop, which led to mechanical hypersensitivity after RCS. Since extracellular matrix proteoglycan and acid sensitive ion channels (TRPV1 and ASIC3) have been considered as possible mechanisms for sensitizing/activating nociceptors by protons, we investigated their involvement. Manipulating the extracellular matrix proteoglycan with chondroitin sulfate and chondroitinase ABC reversed the MMWT decrease after RCS, supporting the involvement of the extracellular mechanism. Inhibiting ASIC3, but not TRPV1, reversed the decreased MMWT after RCS, and ASIC3 mRNA and protein in the dorsal root ganglia were upregulated, indicating ASIC3 involvement. These findings suggest that extracellular mechanism and ASIC3 play essential roles in proton-induced mechanical hyperalgesia after RCS.
The TIGIT+ T regulatory cells subset associates with nosocomial infection and fatal outcome in COVID-19 patients under mechanical ventilation
Authors: de Lima Mikhael Haruo Fernandes , Machado Caio Cavalcante , Nascimento Daniele Carvalho , Silva Camila Meirelles S. , Toller-Kawahisa Juliana Escher , Rodrigues Tamara Silva , Veras Flavio Protassio , Pontelli Marjorie Cornejo , Castro Italo A. , Zamboni Dario Simões , Filho José-Carlos A. , Cunha Thiago M. , Arruda Eurico , da Cunha Larissa Dias , Oliveira Renê D. R. , Cunha Fernando Q. , Louzada-Junior Paulo 
Published: August 2023

AbstractThe TIGIT+FOXP3+Treg subset (TIGIT+Tregs) exerts robust suppressive activity on cellular immunity and predisposes septic individuals to opportunistic infection. We hypothesized that TIGIT+Tregs could play an important role in intensifying the COVID-19 severity and hampering the defense against nosocomial infections during hospitalization. Herein we aimed to verify the association between the levels of the TIGIT+Tregs with the mechanical ventilation requirement, fatal outcome, and bacteremia during hospitalization. TIGIT+Tregs were immunophenotyped by flow cytometry from the peripheral blood of 72 unvaccinated hospitalized COVID-19 patients at admission from May 29th to August 6th, 2020. The patients were stratified during hospitalization according to their mechanical ventilation requirement and fatal outcome. COVID-19 resulted in a high prevalence of the TIGIT+Tregs at admission, which progressively increased in patients with mechanical ventilation needs and fatal outcomes. The prevalence of TIGIT+Tregs positively correlated with poor pulmonary function and higher plasma levels of LDH, HMGB1, FGL2, and TNF. The non-survivors presented higher plasma levels of IL-33, HMGB1, FGL2, IL-10, IL-6, and 5.54 times more bacteremia than survivors. Conclusions: The expansion of the TIGIT+Tregs in COVID-19 patients was associated with inflammation, lung dysfunction, bacteremia, and fatal outcome.
Roles unveiled for membrane-associated mucins at the ocular surface using a Muc4 knockout mouse model
Authors: Martinez-Carrasco Rafael , Rachagani Satyanarayan , Batra Surinder K. , Argüeso Pablo , Fini M. Elizabeth 
Published: August 2023

AbstractMembrane-associated mucins (MAMs) are proposed to play critical roles at the ocular surface; however, in vivo evidence has been lacking. Here we investigate these roles by phenotyping of a Muc4 KO mouse. Histochemical analysis for expression of the beta-galactosidase transgene replacing Muc4 revealed a spiraling ribbon pattern across the corneal epithelium, consistent with centripetal cell migration from the limbus. Depletion of Muc4 compromised transcellular barrier function, as evidenced by an increase in rose bengal staining. In addition, the corneal surface was less smooth, consistent with disruption of tear film stability. While surface cells presented with well-developed microprojections, an increase in the number of cells with fewer microprojections was observed. Moreover, an increase in skin-type keratin K10 and a decrease in transcription factor Pax6 was observed, suggesting an incipient transdifferentiation. Despite this, no evidence of inflammatory dry eye disease was apparent. In addition, Muc4 had no effect on signaling by toll-like receptor Tlr4, unlike reports for MUC1 and MUC16. Results of this study provide the first in vivo evidence for the role of MAMs in transcellular barrier function, tear film stability, apical epithelial cell architecture, and epithelial mucosal differentiation at the ocular surface.
Performance analysis of channel estimation techniques for IRS assisted MIMO
Authors: Baye Alelign Ewinetu 
Published: August 2023

AbstractThe need for low latency and high data rates is increasing rapidly since the advent of wireless communication. The current fifth-generation (5G) networks are unable to fulfill the requirements of upcoming technologies. So, researchers are commencing their research beyond 5G. Terahertz (THz) frequency is one candidate to satisfy the large bandwidth requirement and intelligent reflecting surface (IRS) is incorporated to mitigate signal blockage which is the main problem for communication at high frequencies. Channel estimation is a process of identifying coefficients of the channel matrix. The compressive sensing technique is of great importance as it decreases the number of pilot symbols required for channel estimation. As mmWave and THz signals are naturally sparse applying a compressive sensing technique is reasonable. Unlike other papers, this paper considers the imperfect IRS elements, which is the real case, by varying the value of $$\beta$$
β
(amplitude perturbations). The channel estimation performance of the conventional least squares (LS), orthogonal matching pursuit (OMP) and Oracle is analyzed with respect to signal-to-noise ratio (SNR) and pilot length (T). Normalized mean square error (NMSE) and spectral efficiency (SE) are used as performance metrics and the OMP algorithm is found to perform better than LS even at a fewer number of pilot symbols.
A data-driven approach to identify a rapid screener for auditory processing disorder testing referrals in adults
Authors: Cancel Victoria E. , McHaney Jacie R. , Milne Virginia , Palmer Catherine , Parthasarathy Aravindakshan 
Published: August 2023

AbstractHearing thresholds form the gold standard assessment in Audiology clinics. However, ~ 10% of adult patients seeking audiological care for self-perceived hearing deficits have thresholds that are normal. Currently, a diagnostic assessment for auditory processing disorder (APD) remains one of the few viable avenues of further care for this patient population, yet there are no standard guidelines for referrals. Here, we identified tests within the APD testing battery that could provide a rapid screener to inform APD referrals in adults. We first analyzed records from the University of Pittsburgh Medical Center (UPMC) Audiology database to identify adult patients with self-perceived hearing difficulties despite normal audiometric thresholds. We then looked at the patients who were referred for APD testing. We examined test performances, correlational relationships, and classification accuracies. Patients experienced most difficulties within the dichotic domain of testing. Additionally, accuracies calculated from sensitivities and specificities revealed the words-in-noise (WIN), the Random Dichotic Digits Task (RDDT) and Quick Speech in Noise (QuickSIN) tests had the highest classification accuracies. The addition of these tests have the greatest promise as a quick screener during routine audiological assessments to help identify adult patients who may be referred for APD assessment and resulting treatment plans.