bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mutational spectrum of breast cancer by shallow whole-genome sequencing of cfDNA and tumor gene panel analysis
Authors: Ambriz-Barrera Fernando , Rojas-Jimenez Ernesto , Díaz-Velásquez Clara Estela , De-La-Cruz-Montoya Aldo Hugo , Martínez-Gregorio Héctor , Ruiz-De-La-Cruz Miguel , Huertas Antonio , Montealegre Ana Lorena , Castro-Rojas Carlos , Acosta Gabriela , Vaca-Paniagua Felipe , Perdomo Sandra 
Published: June 2023

Abstract
Breast cancer (BC) has various molecular subgroups with differing risks and treatments. Tumor biopsies for BC detection are invasive and may not reflect tumor heterogeneity. Liquid biopsies have become relevant because they might overcome these limitations. We evaluated the feasibility to detect somatic copy number alterations (SCNAs) in BC using shallow whole genome sequencing (sWGS) in cfDNA from archived samples from National Cancer Institute of Colombia patients. We sequenced tumor tissues from 38 BC patients with different molecular subtypes using a panel of genes, and sWGS on 20 paired cfDNA samples to detect SCNAs and compare with the tumor samples. We identified an extensive intertumoral heterogeneity between the molecular subtypes of BC, with a mean tumor load of 602 mutations in the gene panel of tumor tissues. There was a 12.3% of concordance in deletions in the cfDNA-tumor pairs encompassing seven genes: BRCA1, CDK12, NF1, MAP2K4, NCOR1, TP53, and KEAP1 in 3 patients. Amplifications were only detected in tumor tissues. This study shows the feasibility to detect SCNAs in BC using sWGS in cfDNA. This strategy may improve tumor SCNA events detection, complementing tumor tissue biopsies, and facilitating a wider identification of potential therapeutic targets.
Strategies to Preventing pressure injuries among intensive care unit patients mechanically ventilated in prone position: a systematic review and a Delphi study
Authors: Wang Zonghua , Fan Jiangshan , Chen Ling , Xie Langlang , Huang Lingfang , Ruan Yang , Xu Xia , Liang Zeping 
Published: June 2023

Abstract
Background
Although the incidence of pressure injury in the prone position is high for the mechanically ventilated patients in the intensive care unit, evidence-based strategies are still lacking.
Propose
To conduct a systematic review of current evidence, and to propose a series of strategies to prevent pressure injuries among mechanically ventilated patients with prone position in the intensive care unit.
Method
The study was guided by the Medical Research Council framework. After a systematic review of current evidence of original articles, guidelines, expert consensus and theories, a strategy draft was developed. Then we invited 20 experts to modify and refine these strategies through two rounds of Delphi consensus method.
Result
After two rounds of Delphi process, the importance of coefficient of variation (Cv) and Kendall’s coefficient of concordance in the strategies repository were 0.067 and 0.311, respectively. And the operability of Cv and Kendall’s coefficient of concordance in the strategy draft was 0.055 and 0.294, respectively. Ultimately, we established 31 strategies for including 7 themes (assess risk factors, assess skin and tissue, body position management, skin care, nutrition, preventing medical device-related pressure injuries, education and supervision).In addition, we also developed a strategy framework to clarify our strategies.
Conclusion
According to the Medical Research Council framework, we developed 7 themes and 31 strategies to prevention prone-position pressure injuries among the intensive care unit mechanically ventilated patients. This study was considered to improve the clinical management of pressure injuries among prone position patients in the intensive care unit settings.
Efficacy of rTMS for poststroke epilepsy and its effects on patients' cognitive function and depressive status
Authors: Hu Minting , Qin Bailing , Li Tong , Wei Chunyan , Su Dajing , Tan Zuocai 
Published: June 2023

Abstract
OBJECTIVE: This stud aimed to investigate the efficacy of rTMS in the treatment of poststroke epilepsy and the effect of rTMS on patients' cognitive function and depressive status.
METHODS: One hundred and twenty-one poststroke epilepsy patients with mild cognitive impairment and depressive status admitted to the Department of Neurology of the Second People's Hospital of Nanning from January 1, 2017, to April 31, 2023, were selected and divided into the rTMS treatment group (treated group) and the control group. MMSE scores and HAMD scores were recorded before and after treatment. The frequency of EEG spiky and sharp waves recorded before and after treatment within 24 hours and the frequency of any clinical seizure form (the number of clinical seizures within 1 month after treatment) and changes in observed indices before and after treatment were calculated. The differences between the data of the two groups of scales were analyzed to further assess the efficacy of rTMS in the treatment of poststroke epilepsy and its effects in terms of cognition and depression.
RESULTS: Compared with drug treatment alone, rTMS significantly decreased clinical seizures and epileptiform discharges after stroke, especially in patients with lesions in the frontal, temporal, and parietal lobes. Compared with drug treatment alone, rTMS treatment can effectively reduce cognitive impairment and mood disorders, such as depression, especially for patients with lesions in the frontal and temporal lobes. The results of this experiment suggest that rTMS treatment does not increase adverse effects.
CONCLUSION: rTMS reduces clinical seizures while improving cognitive impairment and depression in patients with epilepsy. Therefore, we suggest that low-frequency rTMS can be used as an adjunctive treatment for patients with epilepsy and provide some ideas and references for the treatment of epilepsy with cognitive impairment and depression.
Intermittent fasting reverses age- and obesity- associated adipose fibrosis through circadian reprogramming of adipose precursor cells.
Authors: Lee Ju Hee , Patel Yash , Yeung Joanna Lan-Hing , Zhang Qiwei , Pickel Lauren , He Xiaolin , Ealey Kafi , Togo Jacques , Kim Yun Hye , Kim Kyoung-Han , Park Jin Gyoon , Jackson Timothy , Okrainec Allan , Yuen Darren , Kim Jae-Ryong , Park So-Young , Dash Satya , Sung Hoon-Ki 
Published: June 2023

Abstract
Aging is a crucial factor for tissue fibrosis that is closely associated with the functional decline of various organs. Fibrosis of white adipose tissue (WAT) is a hallmark of dysfunctional WAT that is directly linked to age- and obesity-associated metabolic abnormalities. Recent studies have highlighted the role of dysfunctional adipose progenitor cells (APCs) in WAT fibrosis and impaired adaptive tissue plasticity, leading to systemic insulin resistance. However, therapeutic options for WAT fibrosis are limited. Intermittent fasting (IF) is an effective dietary regimen for weight control and metabolic improvement through various mechanisms. Here, we show that IF confers therapeutic benefits in aged and obese mice through the reduction of WAT fibrosis. Single-cell analyses revealed that IF significantly reduces fibrotic signatures within APCs along with the upregulation of circadian pathways. Importantly, mice lacking a core circadian gene exhibited increased fibrotic signatures in WAT and diminished beneficial response to IF, indicating the requirement of functional circadian rhythm in IF-induced WAT remodeling. Lastly, we observed that a dysregulated circadian rhythm in human APCs is associated with WAT fibrosis and insulin resistance. Collectively, our findings highlight the novel role of the APC circadian rhythm in the plasticity of WAT and the metabolic response to IF.
Neurocognitive Responses to Spatial Design Behaviors and Tools Among Interior Architecture Students: A Pilot Study
Authors: Şekerci Yaren , Kahraman Mehmet Uğur , Kaya Ümran , Özturan Özgü , Çelik Ertuğrul , Akay Diyar , Ayan Sevgi Şengül 
Published: June 2023

Abstract
The impact of emotions on human behavior is substantial, and the ability to recognize people's feelings has a wide range of practical applications including education. Here, the methods and tools of education are being calibrated according to the data gained over electroencephalogram (EEG) signals. The issue of which design tools would be ideal in the future of interior architecture education, is an uncertain field. It is important to measure the students’ emotional states while using manual and digital design tools to determine the different impacts. Brain-computer interfaces have made it possible to monitor emotional states in a way that is both convenient and economical. In the research of emotion recognition, EEG signals have been employed, and the resulting literature explains basic emotions as well as complicated scenarios that are created from the combination of numerous basic emotions. Linguistic summarization is a technique that can assist in extracting knowledge from huge volumes of data by identifying pre-defined structures and expressing them in a concise manner. The purpose of this research is to investigate the attachment and emotional states of interior architecture students while they are designing in 2D or 3D with manual or digital tools, as well as to investigate differences in the use of design tools and behaviors that are based on teaching methodologies. The study produced summaries of the data using a fuzzy sets-based linguistic summarization technique, and it explained the findings in daily natural language that is easy to understand without expert.
A Deep Dive into the Hippocampal Proteome in a Rat Model of Hepatic Encephalopathy Unveils the Role of FAK1 and Tetraspanins in its Pathogenesis
Authors: Prasad Shambhu Kumar , Singh Vishal Vikram , Acharjee Arup , Acharjee Papia 
Published: June 2023

Abstract
Hepatic Encephalopathy (HE) is a debilitating neurological disorder associated with liver failure, characterized by impaired brain function. This study aimed to elucidate the molecular underpinnings of HE and identify potential therapeutic targets. A model of moderate HE was induced in rats using thioacetamide, simulating the liver damage observed in patients. We employed LC-MS/MS-based label-free proteomics to quantitatively profile hippocampal proteins, identifying 2175 proteins, 47 of which exhibited significant alterations in the HE condition. Utilizing Metascape, a network analysis tool, we discovered that biological pathways integral to brain function, including gliogenesis, modulation of chemical synaptic transmission, astrocyte differentiation, regulation of organ growth, and response to cAMP, myelination, synaptic function, were disrupted during HE. The STRING database further elucidated protein-protein interaction patterns among the differentially expressed proteins. Our findings highlight Focal Adhesion Kinase 1 (FAK1) and its associated tetraspanins (Tetraspanins 2 and CD9) as key contributors to the neurological dysfunction observed in HE. Additionally, we noted alterations in cellular morphology and neuronal arborization in the hippocampal neurons of HE rats, suggesting these proteins influence synaptic communication and memory storage. This study provides novel insights into the molecular mechanisms driving HE and paves the way for identifying novel therapeutic targets for improved disease management.
Comparing Outcomes of Fetal Growth Restriction Defined by Estimated Fetal Weight versus Isolated Abdominal Circumference
Authors: Griffin Myah , Mehta-Lee Shilpi , Penfield Christina , Roman Ashley 
Published: June 2023

Abstract
Purpose
The objective of this study was to compare maternal and neonatal outcomes when diagnosis of FGR was based on isolated abdominal circumference < 10th percentile for gestational age (GA) (iAC group) versus overall estimated fetal weight < 10th percentile (EFW group).
Methods
This was a retrospective cohort study of singleton gestations who underwent growth ultrasounds and delivered at a single health system from 1/1/19 − 9/4/20. The study group was comprised of patients with AC < 10th percentile and EFW ≥ than the 10th percentile (iAC group). The control group included patients with overall EFW < 10th percentile (EFW group). Outcomes evaluated included GA at delivery, mode of delivery, fetal and neonatal outcomes. Data was analyzed using Mann Whitney U, X2, and Fisher exact tests with significance defined as p < 0.05.
Results
635 women met inclusion criteria, 259 women in the iAC group and 376 women in the EFW group. The iAC group was noted to have a later GA at diagnosis and delivery. iAC was associated with lower rates of preterm birth (PTB), NICU admission, SGA at delivery and umbilical artery cord gas < 7.0.
Conclusion
Using iAC as a definition of FGR increased the number of FGR cases by 1.69-fold over EFW criteria alone. However, obstetrical and neonatal outcomes for the iAC group appear to be significantly better than those in the EFW group, with low rates of PTB, NICU admission, and umbilical artery cord gas < 7.0.
Cross-cultural Adaptation and Validation of the KOOS, JR Questionnaire for Assessing Knee Osteoarthritis in Spanish-speaking Patients
Authors: Guiloff Rodrigo , Iñiguez Magaly , Prado Tomás , Figueroa Francisco , Olavarría Nicolás , Carrasco Eduardo , Ergas Enrique , Lyman Stephen , Salgado Martín 
Published: June 2023

Abstract

Background
The Knee Injury and Osteoarthritis Outcome Score, Joint Replacement (KOOS, JR), a validated short form (7 questions) from the original KOOS score (42 questions), has demonstrated higher response rate among other patient-reported outcome measures (PROMs) in patients with knee osteoarthritis (OA). However, there is no validated Spanish version. The present study aims to translate, adapt, and validate a Spanish version of the KOOS, JR, including a reliability and validity analysis.
Methods
Prospective validation study following the six stages of the "Guidelines for the Process of Cross-Cultural Adaptation of Self-Report Measures". Stage I: Translation from the original English version to Spanish by two native Spanish speakers. Stage II: Synthesis with linguistic and legibility test. Stage III: Back translation by two blinded native English speakers. Stage IV: Expert committee review of the final Spanish version. Stage V: Pretesting in 10 volunteers with similar demographic characteristics as the target study population. Stage VI: Approval by the expert committee and the original developer. Psychometric testing was conducted in a new group of participants: patients with knee OA. Subjects answered the Spanish KOOS, JR (S-KOOS, JR), and a validated Spanish Oxford Knee Score (S-OKS). Re-test were conducted at 10 days. Acceptability, floor and ceiling effect, internal consistency (Cronbach’s α), reproducibility (mixed-effect model coefficient [MEMC]), and construct validity (Spearman's correlation; p = 0.05) were assessed.
Results
41 patients (mean age: 65.6 ± 5.39; 48.8% female) participated in the study. All patients (100%) answered both scores during the first assessment and thirty-eight (92.7%) during the second assessment. All PROMs were answered completely (100%). The S-KOOS, JR resulted in 100% acceptability when answered. There were no ceiling or floor effects detected. The Cronbach's α for the S-KOOS, JR was 0.927, and its MEMC was 0.852 (CI 95%: 0.636–1.078). The Spearman's correlation between the S-KOOS, JR and the S-OKS was 0.711 (CI: 0.345–0.608; p < 0.001) and 0.870 (IC: 0.444–0.651; p < 0.001) for the first and second assessments, respectively.
Conclusion
The S-KOOS, JR has very high internal consistency and reproducibility, with a high correlation with the S-OKS; it is a reliable and valid instrument for characterizing Spanish-speaking patients suffering from knee OA.
Barriers and enablers for implementation of 4IR-linked diagnostics models at point-of-care in South Africa: Stakeholder engagement
Authors: Moetlhoa Boitumelo , Nxele Siphesihle R , Maluleke Kuhlula , Mathebula Evans , Marange Musa , Chilufya Maureen , Dzinamarira Tafadzwa , Duah Evans , Dzobo Matthias , Kekana Mable , Jaya Ziningi , Thabane Lehana , Dlangalala Thobeka , Nyasulu Peter , Hlongwana Khumbulani , Dlungwane Thembelihle , Kgatle Mankgopo , Gxekea Nobuhle , Mashamba-Thompson Tivani 
Published: June 2023

Abstract
Fourth industrial revolution (4IR) technologies may improve access to disease diagnosis and treatment at point-of-care (POC). Stakeholder engagements are an important step when implementing such interventions. In this study, we report the findings of a workshop that was held with key stakeholders to determine barriers and enablers for implementing 4IR-linked POC diagnostic models in South Africa. The workshop formed part of the 2022 REASSURED Diagnostics symposium. The nominal group technique (NGT) workshop was conducted in two phases: phase 1 and phase 2 focused on determining barriers and enablers, respectively, to implementing 4IR-linked POC diagnostic models in South Africa. Stakeholders identified connectivity, offline functionality, and load shedding as some of the most important barriers, while ease of use, subsidies by the National Health Insurance, and 24-hour assistance would enable the implementation 4IR-linked POC diagnostic models. The NGT workshop provided a suitable platform for identifying important barriers and enablers to the implementation of 4IR-linked POC diagnostic models. A follow-up study should identify the best strategies for implementing 4IR-linked POC diagnostics models in underserved populations.
Grand Weighted Variable Herz-Morrey Spaces Estimates for Higher Order Commutators of Fractional Integrals
Authors: Liu Ming , Yao Xiaobin 
Published: June 2023

Abstract
In this paper, we establish the boundedness for higher order commutators generated by fractional integral with BMO function Iβ,bm on weighted variable exponent Herz-Morrey spaces MḰq, p(·)}α,λ(ω). At the same time, we also get the boundedness for Iβ,bm on grand weighted variable exponent Herz-Morrey spaces MḰq, p(·)}α,λ(ω).
AMS Subject Classification(2020): 46E30, 47B38
Astragaloside IV inhibiting rotenone-induced α-syn presentation and CD4 T-cell immune response
Authors: Wang Mengdi , Sun Fengjiao , Han Xiaofeng , Wang Nan , Liu Yalan , Cai Jinfeng , Tong Shanshan , Wang Rui , Wang Hongcai 
Published: June 2023

Abstract
Background The increased α-synuclein (α-syn)-dependent activation of CD4 T cells leads to the progressive loss of Parkinson’s disease (PD) dopaminergic (DA) neurons in the substantia nigra (SN). Astragaloside IV (AS-Ⅳ) protects DA neurons against neuroinflammation. The effects of AS-IV on CD4 T-cell-mediated immune responses in PD remain to be defined.Methods Rotenone (ROT) injected unilaterally into the substantia nigra compact part (SNc) of rats induced PD. AS-Ⅳ (20 mg/kg) was intraperitoneally injected once a day for 14 days. The limb hanging test and rotarod test were performed to evaluate the alteration of behavior at 4 and 6 weeks. Total gastrointestinal transit tests were performed at 4 weeks. Western blotting was used to detect the expression of proinflammatory cytokine proteins. Immunofluorescence staining was conducted to test the expression and localization of major histocompatibility complex class Ⅱ (MHCⅡ), cleaved caspase-1 and α-syn in astrocytes. Flow cytometry analysis, immunohistochemistry and immunofluorescence staining were used to measure the expression of CD4 T-cell subsets in the SN.Results The application of AS-IV protected against the loss of DA neurons and behavioral deficits in ROT-induced PD rat models. AS-Ⅳ administration inhibited the aggregation of α-syn in DA neurons and the expression of proinflammatory cytokines such as TNF-α, IL-18 and IL-1β. AS-Ⅳ decreased the activation of CD4 T cells and three CD4 T-cell subsets: Tfh, Treg and Th1. AS-Ⅳ interrupted the ROT-induced interaction between astrocytes and CD4 T cells and the colocalization of MHCⅡ and α-syn in astrocytes. AS-Ⅳ inhibited the expression of α-syn in astrocytes and the colocalization of α-syn and cleaved caspase-1 in astrocytes.Conclusion AS-Ⅳ prevents the loss of DA neurons in PD by inhibiting the activation of α-syn-specific CD4 T cells, which is regulated by MHCⅡ-mediated antigen presentation in astrocytes.
Investigation of the Influence of a Single Fissure on the Heat Storage/Release Characteristics of Backfill Coupled Heat Exchangers in Underground Stopes
Authors: Zhang Bo , Zhan Rui , Liu Lang , Huan Chao , Zhao Yujiao , Zhang Xiaoyan 
Published: June 2023

Abstract

With the progressive depletion of shallow mineral resources and the increasing mining depth, the challenges posed by high well temperatures have become more prominent. To address this issue, both domestic and foreign research institutions have proposed a synergistic mining model that combines mineral deposits and geothermal resources. However, in deep mines, where high temperature, high stress, and high permeability pressures prevail, the long-term cyclic heat storage/release operations inevitably lead to fracture damage in the buried pipe filling body. Consequently, this damage alters the heat transfer performance of the system. This paper aims to establish a comprehensive three-dimensional mathematical model of a fissured buried tube-filler system by employing a combination of experimental tests and numerical simulations. The research focuses on the effects of fracture location, fracture width, and fracture cracking degree on various aspects, including temperature distribution, heat storage/release, outlet water temperature of the buried pipe, and the efficiency of the actual-sized buried pipe filler. Importantly, the study is conducted under the condition of no seepage flow. The results reveal several key findings. Firstly, in the case of bilateral heating, the proximity of the fissure to the heating side greatly influences the temperature distribution within the filling body, while the fissure located away from the heating side has a lesser impact. Secondly, the presence of fissures significantly hampers the heat transfer performance of the filling body. As the fissure width increases from 1mm to 10mm, the temperature drop during heat storage reaches 8.28%, and during heat release, it reaches 14.81%. Lastly, the average body temperature of the specimens decreases as the degree of cracking increases. After completing the heat release process, the average body temperature of fissure-free specimens and fully cracked specimens is recorded as 39.01°C and 35.82°C, respectively, representing a decrease of 8.17% in average body temperature.
Ultrasound Assessment of Infrapatellar and Sartorial Branches of the Saphenous Nerve: Establishing a Safety Zone for Preventing Nerve Injury in Knee Surgery and Injections
Authors: Kim Byungjun , Kang Kyung Hun , Yoon Joon Shik 
Published: June 2023

Abstract
Purpose
Our study aimed to illustrate the positional relationship of the two branches of the saphenous nerve: the infrapatellar branch of the saphenous nerve (IPBSN) and sartorial branch of the saphenous nerve (SBSN), as well as the anatomical landmarks using high-resolution ultrasound (HRUS) to help prevent iatrogenic nerve injury.
Methods
We used HRUS to explore the positional relationships among the anatomical landmarks, IPBSN, and SBSN in 40 knees of 20 participants. The distances from these branches to key reference points were recorded. Using the ultrasound caliper mode, we measured the depth from the skin surface to the nerves at four distinct points.
Results
The average distances between IPBSN and medial border of patella (MBP) and IPBSN and medial border of patellar ligament (MBPL) were 47 ± 7 mm and 42 ± 9 mm, respectively. SBSN showed mean distances of 94 ± 9 mm and 96 ± 9 mm to MBP and MBPL, respectively. The mean distance from the upper edge of pes anserine to IPBSN at the patellar apex (PA) level was 24 ± 10 mm and to SBSN was 34 ± 9 mm.
Conclusion
We used high-resolution ultrasound to evaluate IPBSN and SBSN and their positions relative to anatomical landmarks. The study results offer valuable insights into the course of these nerves, which can help establish a safety zone to prevent accidental nerve injuries during knee surgeries and injections.