bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of the COVID-19 Pandemic on Early Intervention Utilization and Need for Referral after NICU Discharge in VLBW Infants
Authors: David Jieun , Wambach Caroline , Kraemer Megan , Johnson Tricia , Greene Michelle , Lee Esther , Patra Kousiki 
Published: February 2023

AbstractObjective:To examine the impact of COVID-19 pandemic on early intervention (EI) services in VLBW infants.Study Design:208 VLBW infants seen in NICU FU pre-COVID-19 were compared to 132 infants seen during COVID-19 at 4, 8 and 20 months corrected age (CA) in terms of: enrollment in Child and Family Connections (CFC; intake agency for EI), EI therapies, need for CFC referral and Bayley scores.Results:Infants seen during COVID-19 at 4, 8 and 20 months CA were 3.4 (OR, 95% CI 1.64, 6.98), 4.0 (1.77, 8.95) and 4.8 (2.10, 11.08) times more likely to need CFC referral at FU based on severity of developmental delay. Infants followed during COVID-19 had significantly lower mean Bayley cognitive and language scores at 20 months CA.Conclusions:VLBW infants seen during COVID-19 had significantly higher odds of needing EI and significantly lower cognitive and language scores at 20 months CA.
Spatiotemporal monitoring demonstrates prebiotic treatment
responses in a periodontal multispecies biofilm model
Authors: Bernaerts Kristel , Ghesquiere Justien , Simoens Kenneth , Koos Erin , Boon Nico , Teughels Wim 
Published: February 2023

Abstract
In vitro periodontal model systems are required for further elucidating phenomena behind periodontal pathogenesis and treatment applications. However, most oral biofilm models apply static conditions and are restricted to end-point measurements. This study established a multispecies periodontal model in the drip flow biofilm reactor in order to mimic shear flow in the oral cavity. The design is re-engineered to enable real-time characterization. A community of five bacteria, Streptococcus gordonii-GFPmut3*, Streptococcus oralis-GFPmut3*, Streptococcus sanguinis-pVMCherry, Fusobacterium nucleatum, and Porphyromonas gingivalis-SNAP26 is visualized using two distinct fluorescent proteins and the SNAP-tag. The biofilm in the reactor develops into a spatially uniform, dense and metabolically active biofilm with relative cell abundances similar to those in a healthy individual. Metabolic activity, structural features and bacterial composition of the biofilm remain stable from 3 up to 6 days. As a proof of concept for our periodontal model, the 3-day developed biofilm is exposed to a prebiotic treatment with L-arginine. Multifaceted effects of L-arginine on the oral biofilm were validated by this model setup. L-arginine showed to inhibit growth and co-aggregations of the pathogenic species, and to reduce biofilm thickness and volume. Additionally, L-arginine is metabolized by Streptococcus gordonii-GFPmut3* and Streptococcus sanguinis-pVMCherry, producing high levels of ornithine in the biofilm. In conclusion, our drip flow reactor setup is promising in studying spatiotemporal behavior of a multispecies periodontal community.
Effect of small peptide chelated iron on growth performance, immunity and intestinal health in weaned pigs
Authors: Sun Limei , Yu Bing , Luo Yuheng , Zheng Ping , Huang Zhiqing , Yu Jie , Mao Xiangbing , Yan Hui , Luo Junqiu , He Jun 
Published: February 2023

Abstract

Background: Small peptide chelated iron (SPCI), a novel iron supplementation in pig diets, owns growth-enhancing characteristics. Although a number of researches have been performed, there is no clear-cut evidence to show the exact relationship between the dose and effects of mall peptide chelated minerals.Therefore, we investigated the effect of dietary supplementation of SPCI at different doses in the growth performance, immunity, and intestinal health in weaned pigs.Methods:Twenty-four weaned pigs were randomly assigned into five groups and feed with basal diet containing different levels of SPCI (0, 50, 75, 100, and 125 mg/kg) .On day 22, D-xylose (0.1 g/kg body weight) was orally administrated to each pigs, and blood samples were collected 1 h later. The tissue and intestinal mucosa samples were collected following.Results:Our results showed that the feed to gain ratio (F/G) decreased andthe digestibility of crude protein increased with SPCI addition (P<0.05). Dietary SPCI supplementation at 100 mg/kg not only increased serum ferritin and transferrin, but also elevated the iron content in tibia and tissues (liver, gallbladder, and kidney)(P<0.05). Dietary SPCI (75-100 mg/kg) addition increased the serum immunoglobulins but decreased the serum malondialdehyde (MDA) and D-lactic acid (P<0.05). The serum insulin-like growth factor I (IGF-I) and glutathione peroxidase (GSH-Px) were also elevated by SPCI addition. Interestingly, SPCI supplementation at 75 mg/kg improved the intestinal morphology and barrier function, as suggested by enhanced villus height and villus height/crypt depth (V/C) in duodenum and ileum (P<0.05), as well asjejunum epithelium tight-junction protein ZO-1. Moreover, SPCI supplementation at 75 and 100 mg/kg increased the activity of duodenal lactase and jejunal sucrase (P<0.05). Importantly, SPCI supplementation at 75 mg/kg elevated the expression levels of critical functional genes such as peptide transporter-1(PePT1), sodium/glucose co-transporter-1 (SGLT1), and zinc transporter 1 (ZnT1) in the ileum mucosa (P<0.05). The expression levels of amino acid transporter-1 (CAT1) also elevated by SPCI added at 100 mg/kg.Conclusions:Dietary SPCI supplementation at 75-100 mg improved growth performance, immunity and intestinal health.
Dynamic Linear Models for analysing time series data in coastal environmental monitoring
Authors: Soudant Dominique , Hernández-Fariñas Tania 
Published: February 2023

Abstract
Global changes have led to a renewed interest in time series of environmental monitoring. In France, for example, the French Research Institute for the Exploitation of the Sea (Ifremer) has been managing for 40 years several networks with hundreds of active sites, with annual to fortnightly sampling frequencies, measuring dozens of variables. These long-term datasets are difficult to analyse due to their characteristics (e.g. missing data, outliers, changes in sampling frequency, shifts).For this large number of time series, this paper proposes a semi-automatic procedure based on Dynamic Linear Models, detailed from data pre-processing (e.g. time unit definition, aggregations, transformations), through model specification, automatic and manual intervention, outlier and shift handling, to model hypothesis testing.When applied to three time series combining the above features, the results showed that missing data and changes in sampling frequency were adequately handled. Outliers and structural breaks were identified automatically, but also added manually. Highlighted shifts were identified as artefactual (e.g. probe drift), anthropogenic (e.g. ministerial decree) and ecological changes (e.g. storm impact).Finally, the presented treatment has been successfully applied routinely to more than 19,000 time series with a common and simple model structure. The broad theoretical framework offered by dynamic linear models opens up fruitful perspectives for improving and extending the results presented here, in particular for dealing with measurement quantification limits and time-varying observation variances.
Endophytic fungus from Handroanthus impetiginosus immobilized on electrospun nanofibrous membrane for bioremoval of bisphenol A
Authors: Conceição João Carlos Silva , Alvarega Augusto D. , Mercante Luiza A. , Correa Daniel S. , Silva Eliane Oliveira 
Published: February 2023

Abstract
The current industrial and human activities scenario has accelerated the widespread use of endocrine-disrupting compounds (EDCs), which can be found in everyday products, including plastic containers, bottles, toys, cosmetics, etc., but can pose a severe risk to human health and the environment. In this regard, fungal bioremediation appears as a green and cost-effective approach to removing pollutants from water resources. Besides, immobilizing fungal cells onto nanofibrous membranes appears as an innovative strategy to improve remediation performance by allowing the adsorption and degradation to occur simultaneously. Herein, we developed a novel nanostructured bioremediation platform based on polyacrylonitrile nanofibrous membrane (PAN NFM) as supporting material for immobilizing an endophytic fungus to remove bisphenol A (BPA), a typical EDC. The endophytic strain was isolated from Handroanthus impetiginosus leaves and identified as Phanerochaete sp. H2 by molecular methods. The successful assembly of fungus onto the PAN NFM surface was confirmed by scanning electron microscopy (SEM). Compared with free fungus cells, the PAN@H2 NFM displayed a high BPA removal efficiency (above 85%) at an initial concentration of 5 ppm, suggesting synergistic removal by simultaneous adsorption and biotransformation. Moreover, the biotransformation pathway was investigated, and the chemical structures of fungal metabolites of BPA were identified by ultra-high performance liquid chromatography - high-resolution mass (UHPLC-HRMS) analysis. In general, our results suggest that by combining the advantages of enzymatic activity and nanofibrous structure, the novel platform has the potential to be applied in the bioremediation of varied EDCs or even other pollutants found in water resources.
Mechanically induced pyroptosis enhanced cardiosphere oxidative stress resistance and metabolism for myocardial infarction therapy
Authors: Li Qi , Wang Yingwei , Zhao Jupeng , Chen Jiamin , Wu Donexue , Zheng Youling , Wu Jiaxin , Liu Jie , Lu Jianlong , Zhang Jianhua , Wu Zheng 
Published: February 2023

Abstract
Cellular oxidative stress resistance and bioactivities showed great significance for long-term survival and cardiac regeneration. Cardiosphere-derived cells (CDCs) are favorable cell sources for myocardial infarction (MI) therapy, but effective culture systems for CDC spheroids, cardiospheres (CSps), cultivation and cell function enhancement are not well established. Here, a liquid crystal substrate, octyl hydroxypropyl cellulose ester (OPC), was developed for CSps production and preconditioning. With unique surface properties and mechanical responsiveness, significantly more size-controllable CSps were acquired using OPC substrate, and the OPC-CSps showed improved cell bioactivities and oxidative stress resistance under the stimulation of mechanical-induced pyroptosis. RNA sequencing and metabolism analysis demonstrated the increased metabolic level and improved mitochondrial function of OPC-CSps. In a rat MI model, OPC-CSps significantly improved long-term cardiac function, promoted angiogenesis, and reduced cardiac remodeling in the 3-month observation. Collectively, this study provides a promising and effective system for preparing massive functional CSps for myocardial infarction therapy.
Understanding camouflaging, stigma, and mental health for autistic people in Japan
Authors: Tamura Masaki , Cage Eilidh , Perry Ella , Hongo Minako , Takahashi Toru , Seto Mikuko , Shimizu Eiji , Oshima Fumiyo 
Published: February 2023

Abstract

Background: Camouflaging refers to behaviors in which autistic individuals hide their autistic characteristics and “pass” as non-autistic people. It is postulated that camouflaging is a response to stigma, and preliminary evidence supports this hypothesis. However, there has been limited research on this topic outside of Western countries. This study sought to replicate and extend previous work examining the relationships between camouflaging, stigma, and mental health in Japanese autistic people.
Methods: Two hundred eighty seven autistic people living in Japan completed an online survey on camouflaging, perceived stigma, individualistic and collective strategies, mental wellbeing, anxiety, social anxiety, and depression. We used multiple regression analysis, mediation analyses, and two-sample t-tests to analyze the collected data.
Results: We found that higher camouflaging was predicted by higher perceived stigma and more individualistic and collective strategy use. Camouflaging mediates the association of stigma with depression, anxiety, and social anxiety (but not wellbeing). Furthermore, autistic people in Japan reported lower perceived stigma, camouflaging, wellbeing, and collective strategy use but more individualistic strategies than those in a Western sample.
Conclusion: Our findings support the hypothesis that camouflaging is a response to stigma and can influence the impact of stigma on mental health. There are also cross-cultural differences worth attending to, and more work around supporting positive autistic identity in Japan would be beneficial.
Spinocerebellar ataxia 38: structure-function analysis shows ELOVL5 G230V is proteotoxic, conformationally altered and a mutational hotspot
Authors: Ferrero Enza , Gregorio Eleonora Di , Ferrero Marta , Ortolan Erika , Moon Young-Ah , Campli Antonella Di , Pavinato Lisa , Mancini Cecilia , Tripathy Debasmita , Manes Marta , Hoxha Eriola , Costanzi Chiara , Pozzi Elisa , Sebastiano Matteo Rossi , Mitro Nico , Tempia Filippo , Caruso Donatella , Borroni Barbara , Basso Manuela , Sallese Michele , Brusco Alfredo 
Published: February 2023

Abstract
Fatty acid elongase ELOVL5 is part of a protein family of multipass transmembrane proteins that reside in the endoplasmic reticulum where they regulate long-chain fatty acid elongation. A missense variant (c.689G > T p.Gly230Val) in ELOVL5 causes Spinocerebellar Ataxia subtype 38 (SCA38), a neurodegenerative disorder characterized by autosomal dominant inheritance, cerebellar Purkinje cell demise and adult-onset ataxia. Having previously showed aberrant accumulation of p.G230V in the Golgi complex, here we further investigated the pathogenic mechanisms triggered by p.G230V, integrating functional studies with bioinformatic analyses of protein sequence and structure. Biochemical analysis showed that p.G230V enzymatic activity was normal. In contrast, SCA38-derived fibroblasts showed reduced expression of ELOVL5, Golgi complex enlargement and increased proteasomal degradation with respect to controls. By heterologous overexpression, p.G230V was significantly more active than wild-type ELOVL5 in triggering the unfolded protein response and in decreasing viability in mouse cortical neurons. By homology modelling, we generated native and p.G230V protein structures whose superposition revealed a shift in Loop 6 in p.G230V that altered a highly conserved intramolecular disulfide bond. The conformation of this bond, connecting Loop 2 and Loop 6, appears to be elongase-specific. Alteration of this intramolecular interaction was also observed when comparing wild-type ELOVL4 and the p.W246G variant which causes SCA34. We demonstrate by sequence and structure analyses that ELOVL5 p.G230V and ELOVL4 p.W246G are position-equivalent missense variants. We conclude that SCA38 is a conformational disease and propose combined loss of function by mislocalization and gain of toxic function by ER/Golgi stress as early events in SCA38 pathogenesis.
A case of syphilis associated with immune reconstitution inflammatory syndrome and review of the literature
Authors: Pipitò Luca , Medaglia Alice Annalisa , Trizzino Marcello , Bonura Silvia , Gioè Claudia , Carlo Paola , Colomba Claudia , Cascio Antonio 
Published: February 2023

Abstract
Background: Immune reconstitution inflammatory syndrome (IRIS) associated with syphilis has rarely been described in HIV-infected patients. Diagnosis can be challenging because it is not always possible to discern it from a recent infection or a worsening of an undiagnosed one.
Case presentation: An HIV-positive 42-year-old man with a poor compliance history of antiretroviral therapy presented at our unit and complained of ocular symptoms. Ocular syphilis diagnosis was posed after initial misdiagnosing with cytomegalovirus infection, and antiretroviral therapy compliance improved after switching to a bictegravir-based regimen. Despite intravenous (IV) penicillin, we observed an initial worsening with the appearance of new skin lesions, and IRIS syphilis was suspected. In the literature, 14 cases of IRIS syphilis are described, all regarding male patients. Seven were HIV naïve to therapy, and 7 HIV-experienced with poor therapy compliance. Basal syphilis serology was negative in ten, with subsequent seroconversion after the development of IRIS. IRIS-syphilis development was observed after a median time of 28 days from ART initiation; 10 cases were considered "unmasking-IRIS" and 4 "paradoxical-IRIS". Skin and ocular involvement were the most often reported. In most cases, it was not necessary to use a systemic steroid. A good outcome was reported in 12.
Conclusions: Syphilis should be considered in differential diagnosis with other diseases associated with IRIS. A negative syphilis serology before beginning antiretroviral therapy could convey the impression that syphilis has been ruled out. Whereas a high index of suspicion should be maintained when symptoms suggestive of syphilis, such as ocular and skin manifestations, are noticed after therapy has begun.
One-year longitudinal study of the stratum corneum proteome of retinol and all trans retinoic acid-treated human skin: an orchestrated molecular event
Authors: Abed Kahina , Foucher Aude , Bernard Dominique , Tancrède-Bohin Emmanuelle , Cavusoglu Nukhet 
Published: February 2023

Abstract
Topically applied all trans retinoic acid (RA) is a gold-standard anti-aging molecule used in dermatology. As its cosmetic counterpart used in anti-aging, Retinol (RO) is also a known metabolic precursor of RA. Despite this metabolic link, they haven’t been compared exhaustively in vivo at a mechanistic level. Therefore, to highlight the effect of a topical application of both molecules on in vivoskin, we undertook a longitudinal 1-year study and performed an untargeted proteomic analysis to get a more holistic view on the underlying biological mechanisms of action. The generation of the temporal proteomics signatures of retinol and retinoic acid reveals the impact of these molecules on biological functions related to the aging of skin. New biological functions impacted by retinoids were discovered: glycan metabolism and protein biosynthesis. In addition, the temporal analysis reveals highest modulations at early time points while the physical measures, such as epidermal thickening, was only observed at the latest time point, demonstrating a strong time lapse between molecular and morphological impacts. Finally, these global temporal signatures could be used to identify new cosmetic compounds of interest.
Effect of Balloon Pulmonary Valvuloplasty on Growth of Pulmonary Annulus in Infants with Tetralogy of Fallot
Authors: Waidee Khwaunrat , Lekchuensakul Sarin , Lertsapcharoen Pornthep , Numchaisiri Jule , Benjacholamas Vichai 
Published: February 2023

Abstract
Background
Percutaneous balloon pulmonary valvuloplasty (PBPV) is an alternative intervention in infants with Tetralogy of Fallot (TOF). It can not only improve hypoxia but also promote pulmonary annulus (PA) growth.
Materials and methods
In this study, we evaluated the effect of PBPV on PA growth in infants with TOF. To eliminate the effect of the systemic to pulmonary shunt (SPS) that may promote PA growth, we divided TOF infants into 2 groups; group A, patients who underwent PBPV with or without other SPS, and group B, patients who attempted SPS but without PBPV.
Results
Sixty patients were included, 28 patients in group A and 32 patients in group B. Age at the time of intervention in group A (range, 0.4–5.4; median 1.4 months) was lower than in group B (range, 2.3–7.7; median 4.8 months), p-value 0.02. The body weight in group A (range, 3-5.5; median 3.7 kg) was also lower than in group B (range 4.1–6.4; median 5.9 kg), p-value 0.02. Echocardiographic data at the mean follow-up period of 37.2 months (3–88 months) in group A and 39.6 months (6–95 months) in group B demonstrated an increase in mean PA diameter from 5.0±1.3 mm to 10.2±2.9 mm, p-value < 0.001 in group A; and from 6.2±2 mm to 9.5±2.9 mm, p-value < 0.001 in group B. The median PA z-score increased from − 3.4SD (-3.9 to -2.6SD) to -1.8SD (-2.5 to -0.8SD), with the p-value of 0.002 in group A; and increased from − 2.9SD (-4.5 to -1.3SD) to -2.7SD (-3.6 to -1.4SD), with the p-value of 0.73 in group B. By using the PA z-score as the absolute value, there was a statistically significant increase in the PA z-score during follow-up in group A, but not in group B.
Conclusion
Balloon pulmonary valvuloplasty in infants with TOF can facilitate the growth of pulmonic annulus even after eliminating the effect of the systemic to pulmonary shunt.
Loss ratio of the Capitation Payment Unit of the Health-Promoting Entities in Colombia between 2017 and 2021: A financial–actuarial approach
Authors: Espinosa Oscar , Rodríguez Jhonathan , Urdinola B. Piedad , Nascimento Pedro-Luis Do , Sánchez Alejandra , Arias Martha-Liliana , Valdez Emiliano , Cheng Terence , Fisher Sara-Ellison 
Published: February 2023

Abstract
Background: Because of a change of government, the Colombian Ministry of Health and Social Protection is in the process of presenting a structural reform for the General System of Social Security in Health (GSSSH), in order to implement a ‘preventive and predictive health model’. However, it will always be relevant to review and analyze the fiscal implications of any proposed public policy program, to protect financial sustainability and to promote the better functioning of the system in question.
Methods: To contribute to this topic, we have calculated, using a financial–actuarial approach, the loss ratio for the years 2017 to 2021 for the Capitation Payment Unit (CPU) for all the Health-Promoting Entities (HPE) for both contributory and subsidized schemes. This information, derived from public reports available on the official website of the National Health Superintendency, allows us to estimate the financial burden of the institutions that guarantee access to and provision of health services and technologies in Colombia.
Results: The study shows that close to half of the HPEs in Colombia (which represent 11.6 million affiliates) have CPU loss ratios of more than 100% for the year 2021, evidencing insufficient resources for the operation of health insurance.
Conclusions: Finally, we propose some policy recommendations regarding the strengthening of informed decision-making to allow the healthy financial sustainability of the Colombian GSSSH.