bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression and functional analysis of ace1 and ace2 reveal their differential roles in larval growth and insecticide sensitivity in Spodoptera frugiperda
Authors: Gao Jie , Gong Li-Feng , Wang Huan-Huan , Zhao Rui , Xiao Xing , Tian Xin-Yao , Li Bo , Liang Pei , Gao Xi-Wu , Gu Shao-Hua 
Published: January 2023

Abstract
Acetylcholinesterase (AChE, EC3.1.1.7) is a key enzyme in neuronal signal transduction that hydrolyzes the neurotransmitter acetylcholine (ACh). The toxicological and physiological functions of AChEs in the destructive pest Spodoptera frugiperda, however, are still exclusive. In the present study, two acetylcholinesterase genes, ace1 and ace2, were characterized from S. frugiperda transcriptome and genome. Spatial−temporal expression analysis indicated that both Sfruace1 and Sfruace2 had an enriched expression in the heads among all larval tissues, and in 3rd instar larvae among all developmental stages. Notably, the expression level of Sfruace1 is much higher than Sfruace2 in all tested tissues and developmental stages. The RNA interference (RNAi) with specific designed small interfering RNA (siRNA) significantly reduced the expression of Sfruace1 to 30%, and Sfruace2 to 39%. The knockdown of Sfruace1 expression resulted in mortality of 37.6%, which is significantly higher than 17.06% in the siSfruace2-treated group. Furthermore, the RNAi of Sfruace1 and Sfruace2 expressions reduced the AChE enzymatic activity to 32.81% and 65.77%, respectively, compared with those of the untreated group, 24 h after injection of 70 ng siRNA per insect. The survivors after the siRNA treatments showed an apparent motor retardation to the artificial diet, and an apparent growth inhibition in F0 generation larvae. There was no significant inhibition effect on the growth of F0 and F1 generation pupae and the fecundity of female adult. Insecticide bioassay showed that the siSfruace1-treated larvae were more susceptible to acephate than the siSfruace2-treated and untreated larvae. Our study suggests that Sfruace1 plays a more important role in larval survivorship and susceptibility to acephate than Sfruace2 did in S. frugiperda, and both ace genes may have differential roles in regulating larvae growth, motor ability and insecticidal sensitivity in S. frugiperda.
Proteomic analysis of N-glycosylation of the human placenta between preeclampsia and normal pregnancies
Authors: Qu Guangjin , Luo Qiqi , Hu Pan pan , Huang Kun , Hu Fei fei , Huang Ming li , Luo Shanshun , Li Yue 
Published: January 2023

Abstract
Protein N-glycosylation plays critical roles in modulating placental function, but little is known about N-glycoproteins in the human placenta and modifications in preeclampsia (PE). Here, we show a large, site-specific N-glycoproteome profiling study of PE and normal placenta using quantitative N-glycoproteomics based on mass spectrometry. The study identified disease signatures of altered N-glycoproteins and N-glycosylation site occupancy in PE and provided a system-level view of human placental N-glycoproteins and in vivo N-glycosylation sites. The study led to the discovery of a roster of glycoproteins with aberrant N-glycosylation levels associated with PE, including CD34, ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase family member 1), insulin-like growth factor binding protein (IGFBP3), and HYOU1 (hypoxia up-regulated 1). An emerging phenomenon that N-glycosylation is involved in several PE pathways, including cell adhesion molecules, PI3K-Akt signaling, pyrimidine metabolism, and metabolic pathways was revealed by pathway analysis of PE-associated aberrant glycoproteins. After enzymolysis, the proteins in each group were enriched with N-glycosylated peptides by lectin, the glycochain was excised by peptide N-glycosidase F (PNGase F) in H218O, and the glycosylated sites were analyzed by LC-MS/MS to achieve large-scale qualitative and quantitative analysis of N-glycosylated proteins. Our findings highlight the role of N-glycosylation in the pathogenesis of PE and provide new molecular and system-level insights for understanding and treating this disease.
Synthesis, antitumor evaluation and computational study of thiazolidinone derivatives of dehydroabietic acid-based B ring-fused-thiazole
Authors: Chen Nai-Yuan , Li Cui-Ping , Huang Hong-Fei 
Published: January 2023

Abstract
In an attempt to search for new natural product-based antitumor agents, a series of novel thiazolidinone derivatives of dehydroabietic acid-based B ring-fused-thiazole were designed and synthesized. The primary antitumor tests showed that compounds 5m exhibited almost the best inhibitory activity against the tested cancer cells. The computational study suggested NOTCH1, IGF1R, TLR4, and KDR were the core targets of the title compounds, and the IC50 of SCC9 and Cal27 is strong correlation with the binding ability of TLR4 and compounds.
Bat Mortality in Wind Farms of Southern Europe: Temporal Patterns and Implications in the Current Context of Climate Change
Authors: Salguero María del Mar , Cruz Andrés De la , Gallego Antonio Román Muñoz , Arroyo Gonzalo Muñoz 
Published: January 2023

Abstract
The expansion of renewable energy production, especially wind power, is one of the cornerstones of our strategy for mitigating climate change. However, there is growing concern about the impacts of this energy source on biodiversity, and a need to develop tools to adequately assess this impact and mitigate its effects. In particular, very little is known about the impact on groups of fauna such as bats, which are especially sensitive to environmental changes. We investigated the temporal patterns of bat fatalities in wind farms in the province of Cádiz, in the south of the Iberian Peninsula. An eleven-year data set (2009–2019) from a surveillance program of bird and bat mortality in wind farms was analysed. A total of 2,858 fatalities concerning 10 bat genera were reported, although more than 90% of the affected animals were individuals of the genera Pipistrellus, Eptesicus and Nyctalus. Bat mortality occurred throughout the year, including all winter months in the case of the genus Pipistrellus. Nonetheless, the majority of fatalities of bats with wind turbines in the province of Cádiz occurred during summer and autumn, especially during August. The probability of mortality was positively correlated with the maximum daily temperature. According to the model prediction, the probability of fatality begins to increase slightly from 20°C and then rises sharply when the temperature exceeds 30°C. According to the regional projections of global climate models, an increase in maximum temperatures and the arrival of milder winters may lead to an increase in the annual mortality of bats in wind farms in the coming decades.
Variation in Tree Density, Biomass and Carbon Stock across an Altitudinal Gradient under Large Cardamom Agroforestry System of Darjeeling Himalaya
Authors: . Vineeta , Sarkar B. C. , Tamang M. , Shukla Gopal , N Shahina N , Nath Arun Jyoti , Chakravarty Sumit 
Published: January 2023

Abstract
It has been reported that the large cardamom-based traditional agroforestry systems in the Darjeeling Himalaya of West Bengal, India represents a sustainable land use system with a high potential to store and sequester biomass carbon. Therefore, the present study was undertaken to evaluate the changes in biomass stock and carbon accumulation of this indigenous system along an altitudinal gradient. The study area was classified into three altitude classes as low (700–1200 m asl), mid (1200–1700 m asl) and high (> 1700 m asl). The biomass and carbon storage for low-, mid- and high-altitude classes was estimated at 630.17 and 296.18 Mg ha− 1, 397.05 and 186.61 Mg ha− 1 and 315.78 and 148.42 Mg ha− 1, respectively. Schima wallichi, Cryptomeria japonica and Cupressus cashmeriana were the most dominant species in the low-, mid-, and high altitudinal classes, respectively. The IVI and total biomass of the five dominant species in low-, mid- and high altitudes ranged from 17.34–26.04, 18.15–37.56, 27.13–42.43 and 9.05-133.75 Mgha− 1, 1.38–37.43 Mgha− 1 and 19.0-72.1 Mgha− 1 respectively. Schima wallichi occurred in all the altitudinal classes among the top five dominant species. Across all the altitudinal gradients, the average ecosystem carbon storage was estimated at 295.02 Mg ha− 1. The contribution of SOC to the ecosystem carbon stock increased with the increasing altitude. In contrast, the contribution of biomass carbon to the ecosystem carbon stock decreased with the increase in altitude. The ecosystem carbon stock estimated for the low-altitude class was 36.43% and 45.30% higher than the mid- and high-altitude agroforestry systems. The large cardamom-based agroforestry systems in the Darjeeling Himalayas are thus a prospective carbon sink, both in vegetation and soil due to higher tree densities and natural resource conservation-based traditional farming practices.
Face validity and reliability test of the Danish version of the Compliance Questionnaire Rheumatology
Authors: Knudsen Line Raunsbæk , de Thurah Annette 
Published: January 2023

Abstract
Background Supporting adherence to medication
is an essential part of the treatment and care of patients with
rheumatic and musculoskeletal diseases. The Compliance
Questionnaire Rheumatology (CQR) measures adherence in rheumatic
diseases through 19 items covering drug-taking behaviour to
identify the reasons for adhering to treatment and the factors that
contribute to suboptimal adherence. The objective of this study was
to present the translation of the CQR into Danish and the face
validity and reliability test.

Methods The CQR was translated into Danish according to international guidelines, followed by a face validity test among 10 patients with rheumatoid arthritis. The test–retest reliability of the Danish CQR was evaluated in 49 patients with rheumatoid arthritis using the standard error of the measurement (SEM) converted into the minimally detectable change (MDC) and the intraclass correlation coefficient (ICC). Questionnaires were administered with a minimum of 10 days between assessments.
Results The participants in the reliability test had a mean age of 57.4 years (SD 16.1) and a mean disease duration of 1.13 years (range 2 months–2 years). The mean CQR score in the test and retest was 62.69 (confidence interval (CI) 58.76; 66.63) and 62.51 (CI 58.91; 66.12), respectively, with a SEM of 8.59 (7.16; 10.73) and an MDC of 16.83. A satisfactory test–retest reliability was confirmed by an ICC value of 0.79 (CI 0.68; 0.89).
Conclusion The Danish CQR has satisfactory test–retest reliability in patients newly diagnosed with rheumatoid arthritis and is considered a reliable tool to measure adherence in this group.
The increase in cardiac output induced by a decrease in positive end-expiratory pressure reliably detects volume responsiveness: the PEEP-test study
Authors: Lai Christopher , Shi Rui , Beurton Alexandra , Moretto Francesca , Ayed Soufia , Fage Nicolas , Gavelli Francesco , Pavot Arthur , Dres Martin , Teboul Jean-Louis , Monnet Xavier 
Published: January 2023

Abstract
Background: In patients on mechanical ventilation, positive end-expiratory pressure (PEEP) can decrease cardiac output through a decrease in cardiac preload and/or an increase in right ventricular afterload. Increase in central blood volume by fluid administration or passive leg raising (PLR) may reverse these phenomena through an increase in cardiac preload and/or a reopening of closed lung microvessels. We hypothesized that a transient decrease in PEEP (PEEP-test) may be used as a test to detect volume responsiveness.
Methods: Mechanically ventilated patients with PEEP ≥10 cmH2O (“high level”) and without spontaneous breathing were prospectively included. Volume responsiveness was assessed by a positive PLR test, defined as an increase in pulse-contour-derived cardiac index (CI) during PLR ≥10%. The PEEP-test consisted in reducing PEEP from the high level to 5 cmH2O for one minute. Pulse-contour-derived CI (PiCCO2) was monitored during PLR and the PEEP-test.
Results: We enrolled 64 patients among whom 31 were volume responsive. The median increase in CI during PLR was 14% (11%-16%). The median PEEP at baseline was 12 (10-15) cmH2O and the PEEP-test resulted in a median decrease in PEEP of 7 (5-10) cmH2O, without difference between volume responsive and unresponsive patients. Among volume responsive patients, the PEEP-test induced a significant increase in CI of 16% (12%-20%) (from 2.4±0.7 to 2.9±0.9 L/min/m2, p<0.0001) in comparison with volume unresponsive patients. In volume unresponsive patients, PLR and the PEEP-test increased CI by 2% (1%-5%) and 6% (3%-8%), respectively. Volume responsiveness was predicted by an increase in CI >8.6% during the PEEP-test with a sensitivity of 96.8% (95% confidence interval (95%CI): 83.3%-99.9%) and a specificity of 84.9% (95%CI: 68.1%-94.9%). The area under the receiver operating characteristic curve of the PEEP-test for detecting volume responsiveness was 0.94 (95%CI: 0.85-0.98) (p<0.0001 vs. 0.5). Spearman’s correlation coefficient between the changes in CI induced by PLR and the PEEP-test was 0.76 (95%CI: 0.63-0.85, p<0.0001).
Conclusions: A CI increase >8.6% during a PEEP-test, which consists in reducing PEEP to 5 cmH2O, reliably detects volume responsiveness in mechanically ventilated patients with a PEEP ≥10 cmH2O.
Trial registration
ClinicalTrial.gov (NCT 04023786). Registered July 18, 2019.
Ethics Committee approval CPP Est III (N° 2018-A01599-46)
Recurrent Cushing’s Disease in Adults: Predictors and Long-term Follow-up
Authors: Ünal Mustafa , Selek Alev , Gezer Emre , Canturk Zeynep , Cetinarslan Berrin , Çabuk Burak , Anık İhsan , Ceylan Savaş , koksalan damla , sozen mehmet 
Published: January 2023

Abstract
Purpose
Cushing’s disease (CD) is characterized by endogenous hypercortisolism that is associated with increased mortality and morbidity. Due to high recurrence rates in CD, determination of high-risk patients is of paramount important. In this study, we aimed to determine recurrence rates and clinical, laboratory and histological predictors of recurrence in a high volume single-center.
Methods
This retrospective study included 273 CD patients operated in a single pituitary center between 1997 and 2020. The patients with early postoperative remission were further grouped according to recurrence status (recurrent and sustained remission groups). Demographic, radiologic, laboratory, pathologic and follow-up clinical data of the patients were analyzed and compared between groups.
Results
The recurrence rate was 9.6% in the first 5 years; however, overall recurrence rate was 14.2% in this study. Higher preoperative basal ACTH levels were significantly correlated with CD recurrence even with ACTH levels adjusted for tumor size, Ki-67 levels and tumoral invasion. Recurrence rates were significantly higher in patients with ACTH levels higher than 55 pg/mL, tumor diameter > 9.5 mm, and if adrenal axis recovery was before 6 months. The severity of hypercortisolism, morbidities and demographic factors except age were not predictive factors of recurrence.
Conclusion
Based on our study data, younger age at diagnosis, a diagnosis of osteoporosis, higher preoperative ACTH levels, larger tumor size, invasive behavior, higher Ki 67 index and early recovery of adrenal axis during the postoperative period attracted attention as potential predictors of recurrent disease.
Changes in the equine facial repertoire during different orthopedic pain intensities
Authors: Ask Katrina , Rhodin Marie , Rashid-Engström Maheen , Hernlund Elin , Andersen Pia Haubro 
Published: January 2023

Abstract
A number of facial expressions are associated with pain in horses, however, the entire display of facial activities during orthopedic pain have yet to be described. The aim of the present study was to exhaustively map changes in facial activities in eight resting horses during a progression from sound to mild and moderate degree of orthopedic pain, induced by lipopolysaccharides (LPS) administered in the tarsocrural joint. Lameness progression and regression was measured by objective gait analysis during movement, and facial activities were described by EquiFACS in video sequences (n = 348, total length 892.5 minutes) of the horses obtained when resting in their box stalls. Predictive modeling identified 16 action units and action descriptors, related to ears, eyes, and lower face. Lower lip depressor (AU16), lips part (AU25), half blink (AU47), single ear forward (SEAD101) and single ear rotator (SEAD104) were selected as co-occurring significantly more in horses with pain than in horses without pain. The major change in co-occurring facial activities occurred in the transition from no pain to mild pain. In conclusion, resting horses with induced orthopedic pain showed a dynamic upper and lower facial repertoire and the relationship between level of pain intensity and facial activity appears complex.
Can a powered knee-ankle prosthesis improve weight-bearing symmetry during stand-to-sit transitions in individuals with above-knee amputations?
Authors: Hunt Grace R. , Hood Sarah , Gabert Lukas , Lenzi Tommaso 
Published: January 2023

Abstract
Background: After above-knee amputation, the missing biological knee and ankle are replaced with passive prosthetic devices which are able to dissipate limited amounts of energy using resistive damper systems during “negative energy” tasks like sit-down. However, passive prosthetic knees are not able to provide high levels of resistance at the end of the sit-down movement when the knee is flexed and users need the most support. Consequently, users are forced to over-compensate with their upper body, residual hip, and intact leg, and/or sit down with a ballistic and uncontrolled movement. Powered prostheses have the potential to solve this problem by controlling the joints using motors, which can produce higher levels of resistance at a larger range of joint positions than passive damper systems. Therefore, powered prostheses have the potential to make sitting down more controlled and less difficult for above-knee amputees, improving their functional mobility.
Methods: Ten individuals with above-knee amputations sat down using their prescribed passive prosthesis and a research powered knee-ankle prosthesis. Subjects performed three sit-downs with each prosthesis while we recorded joint angles, forces, and muscle activity from the intact quadricep muscle. Our main outcome measures were weight-bearing symmetry and muscle effort of the intact quadricep muscle. We performed paired t-tests on these outcome measures to test for significant differences between passive and powered prostheses.
Results: We found that the average weight-bearing symmetry improved by 42.1% when subjects sat down with the powered prosthesis compared to their passive prostheses. This difference was significant (p = 0.0012) and every subject’s weight-bearing symmetry improved when using the powered prosthesis. Although the intact quadricep muscle contraction differed in shape, neither the integral nor the peak of the signal was significantly different between conditions (p > 0.01).
Conclusions: In this study, we found that a powered knee-ankle prosthesis significantly improved weight-bearing symmetry during sit-down compared to passive prostheses. However, we did not observe a corresponding decrease in intact-limb muscle effort. These results indicate that powered prosthetic devices have the potential to improve balance during sit-down for individuals with above-knee amputation and provide insight for future development of powered prosthetics.
Hoeflea algicola sp. nov. and Hoeflea ulvae sp. nov., isolated from phycosphere of marine algae
Authors: Kristyanto Sylvia , Kim Jeong Min , Han Dong Min , Jeon Che Ok 
Published: January 2023

Abstract
Two Gram-negative, moderately halophilic, and motile rod bacteria, strains G2-23T and J2-29T, showing catalase- and oxidase-positive activities were isolated from marine algae. Both strains optimally grew at 30°C, pH 7.0, and 2% (w/v) NaCl. Both strains contained ubiquinone-10 as the sole isoprenoid quinone. As major cellular fatty acids and polar lipids, strain G2-23T contained summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, and summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c/ω6c) and phosphatidylethanolamine (PE), phosphatidyl-N-monomethylethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol, sulfoquinovosyl diacylglycerol (SQDG), and an unidentified phospholipid (PL), respectively, while strain J2-29T contained summed feature 8, C18:1 ω7c 11-methyl, and C16:0 and PE, PME, PG, SQDG, and PL, respectively. The genomic DNA G + C contents of strains G2-23T and J2-29T were 59.5 and 62.2 mol%, respectively. Both strains shared 97.9% 16S rRNA gene sequence similarity, 79.8% average nucleotide identity (ANI), and 22.8% digital DNA-DNA hybridisation (DDH) value, indicating that they are different species. Phylogenetic and phylogenomic analyses by 16S rRNA gene and genome sequences, respectively, revealed that strains G2-23T and J2-29T formed different phylogenic lineages within the genus Hoeflea. ANI and digital DDH values between strains G2-23T and J2-29T and other Hoeflea type strains were less than 79.0 and 22.1% and 80.5 and 23.3%, respectively, representing that they are novel species of the genus Hoeflea. Based on the phenotypic, chemotaxonomic, and molecular properties, strains G2-23T and J2-29T represent two different novel species of the genus Hoeflea, for which the names Hoeflea algicola sp. nov. (G2-23T = KACC 22714T = JCM 35548T) and Hoeflea ulvae sp. nov. (J2-29T = KACC 22715T = JCM 35549T), respectively, are proposed.
Effects of Various Copper Sources and Concentrations on Performance, Skeletal Growth, and Mineral Content of Excreta in Broiler Chickens
Authors: Deo Chandra , Biswas Avishek , Sharma Divya , Tiwari Ashok Kumar 
Published: January 2023

Abstract
The experiment was designed to study the effect of supplemental sources and concentrations of copper on the performance and development and mineralization of tibia bones in broiler chickens. 42-day feeding trial using three copper sources i.e., copper sulphate (CuS), copper chloride (CuCl), and copper propionate (CuP) each with four copper levels i.e., 8, 100, 150, and 200 mg/kg was taken. During 4–6 wk of age, the body weight gain was significantly higher at 200 mg Cu/kg diet. There was no significant difference on body weight gain due to interaction between Cu sources and levels. The feed intake during different growth phases did not differ significantly due to either main effect or interaction between copper sources and levels. The feed conversion ratio during 4–6 and 0–6 wk was significantly (P < 0.05) better in CuP supplemented diet (200 mg/kg diet). A total of 72 tibia bones, six per treatment, were collected at the end of experiment. Metabolic trial was conducted to look into mineral retention in broiler chickens on the final three days of the trial (40-42d). 8mg Cu/kg diet with Cu chloride, 100mg Cu/kg diet with Cu propionate, 8mg Cu/kg diet with Cu sulphate, and 8mg/kg diet with Cu propionate supplemented diet resulted in significantly (P < 0.05) increased tibia bone zinc (Zn) levels. Significantly (P < 0.01) lower tibia Zn content was recorded at higher levels of Cu (150 and 200 mg/kg diet). Higher (P < 0.01) tibia Cu content was recorded at 8 mg Cu/kg diet with Cu sulphate treated group. Excreta Zn content was higher (P < 0.01) in Cu sulphate supplemented diet than Cu chloride supplemented diet and lowest excreta Zn content was recorded in Cu propionate supplemented diet. Higher excreta Fe content (P < 0.05) was observed in Cu sulphate and Cu chloride supplemented diet than that recorded in Cu propionate supplemented diet. With the exception of a decrease in the zinc content of the tibia, feeding dietary Cu concentrations up to 200 mg Cu/kg diet, regardless of the different sources, showed no adverse impact on bone morphometry and mineralization parameters.