bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and validation of a novel cuproptosis-related genes signature associated with prognosis, clinical implications and immunotherapy of hepatocellular carcinoma
Authors: He Fengjiao , Zeng Puhua , Ma Sijing , Yang Ximing , Liu Huan , Liu Qiong , Zhou Yangying , Zhu Hong 
Published: February 2023

Background: Cuproptosis is a novel type of regulated cell death and is reported to promote tumor occurrence and progression. However, whether a cuproptosis-related signature has an impact on hepatocellular carcinoma (HCC) is still unclear.Materials and methods: We analyzed the transcriptome data of HCC from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) database, and searched for tumor types with different cuproptosis patterns through consistent clustering of cuproptosis genes. We then constructed a Cuproptosis-Related Genes (CRGs)-based risk signature through LASSO COX regression, and further analyzed its impact on the prognosis, clinical characteristics, immune cell infiltration, and drug sensitivity of HCC.Results: We identified the expression changes of 10 cuproptosis-related genes in HCC, and all the patients can be divided into two subtypes with different prognosis by applying the consensus clustering algorithm. We then constructed a cuproptosis-related risk signature and identified five CRGs, which were highly correlated with prognosis and representative of this gene set, namely G6PD, PRR11, KIF20A, EZH2, and CDCA8. Patients in the low CRGs signature group had a favorable prognosis. We further validated the CRGs signature in ICGC cohorts and got consistent results. Besides, we also discovered that the CRGs signature was significantly associated with a variety of clinical characteristics, different immune landscapes and drug sensitivity. Moreover, we explored that the high CRGs signature group was more sensitive to immunotherapy.Conclusion: Our integrative analysis demonstrated the potential molecular signature and clinical applications of CRGs in HCC. The model based on CRGs can precisely predict the survival outcomes of HCC, and help better guide risk stratification and treatment strategy for HCC patients.
Computational simulation of the effects of interfacial tension in microfluidic flow focusing droplet generators
Authors: Stevens Matthew , Balaur Eugeniu , Abbey Brian 
Published: February 2023

We present simulations of a square flow focusing droplet generator device exploring its performance characteristics over a range of interfacial surface tension values and varying neck width. Droplet generators have a wide range of applications from drug delivery to X-ray diffraction experiments. Matching the droplet frequency and volume to the experimental parameters is critical for maximising the data quality and minimising sample waste. Whilst varying the interfacial surface tension we observed that the lowest frequency of droplets is generated for surface tensions matching those typically reported for water-oil mixtures (around 40 mN/M). Decreasing or increasing the interfacial surface tension, for example by adding surfactant, results in an increase in droplet frequency. We also find that under the conditions simulated here, droplets are generated with much lower capillary numbers and higher Weber numbers than have typically been reported in the literature. The high ratio of flowrate-to-cross-section used here resulted in a velocity which was larger than has previously been reported for flow focusing devices and consequently we observe particularly large associated Reynolds numbers. However, in general, the simulated flow behaviour characteristics most closely match those typically observed for the jetting and tip-streaming regimes. The highest frequency of droplets achieved in our simulated devices was 36 kHz and 56 kHz corresponding to square neck channel widths of 12.5 and 25 µm respectively, an interfacial surface tension of 118.75 mN/m. We also examined the effect of varying neck width geometry for a fixed interfacial surface tension of 52 mN/m. We observed that the highest frequency droplet generation, 61 kHz, corresponded to a neck width of 37.5 µm with a corresponding droplet diameter of 22 µm. The high frequency, high monodispersity, and small droplet size predicted to occur through modification of the interfacial surface tension will have implications for the future design and optimisation of droplet-on-demand microfluidic devices.
Knowledge graph analysis and visualization of artificial intelligence applied in electrocardiogram
Authors: Yang Mengting , Zhang Hongchao , Liu Weichao , Yong Kangle , Xu Jie , Luo Yamei , Zhang Henggui 
Published: February 2023

Background: Electrocardiogram (ECG) provides a straightforward and non-invasive approach for various applications, such as disease classification, biometric identification, emotion recognition, and so on. In recent years, artificial intelligence (AI) shows excellent performance and plays an increasingly important role in electrocardiogram research as well.Objective: This study mainly adopts the literature on the applications of artificial intelligence in electrocardiogram research to focus on the development process through bibliometric and visual knowledge graph methods.Methods: The 2,229 publications collected from the Web of Science Core Collection (WoSCC) database until 2021 are employed as the research objects, and a comprehensive metrology and visualization analysis based on CiteSpace (version 6.1. R3) and VOSviewer (version 1.6.18) platform, which were conducted to explore the co-authorship, co-occurrence and co-citation of countries/regions, institutions, authors, journals, categories, references and keywords regarding artificial intelligence applied in electrocardiogram.Results: In the recent 4 years, both the annual publications and citations of artificial intelligence in electrocardiogram sharply increased. China published the most articles while Singapore had the highest ACP (average citations per article). The most productive institution and authors were Ngee Ann Polytech from Singapore and Acharya U. Rajendra from the University of Technology Sydney. The journal Computers in Biology and Medicine published the most influential publications, and the subject with the most published articles are distributed in Engineering Electrical Electronic. The evolution of research hotspots was analyzed by co-citation references’ cluster knowledge visualization domain map. In addition, deep learning, attention mechanism, data augmentation, and so on were the focuses of recent research through the co-occurrence of keywords.
Physiological analysis of severe chlamydia psittaci pneumonia and clinical diagnosis after doxycycline-based treatment
Authors: He Lujuan , Yang Hongzhong , Liu Shenggang , Liang Weijun , Zhou Zezhi , Long Jing , Wu Jinyang 
Published: February 2023

Objective: To describe the clinical spectrum of severe Chlamydia psittaci pneumonia in order to understand the disease better.Methods: Retrospective analysis was made on 31 patients with severe Chlamydia psittaci pneumonia diagnosed in ICU by next-generation sequencing of metagenome Metagenomic next-generation sequencing(mNGS) from January 2019–November 2022, including clinical characteristics, laboratory examination results, imaging characteristics, treatment, and prognosis.Results: We included 31 patients with severe Chlamydia psittaci pneumonia, 15 of whom had a history of virus exposure. There were 12 cases with multiple bacterial infections, and the common symptoms included fever (31/31,100%), dyspnea (31/31, 100%), cough (22/31, 71.0%), and myalgia (20/31, 64.5%). Laboratory data showed that white blood cells were average or slightly increased, but the levels of C-reactive protein and neutrophils were high. CT findings of the lung were consolidation (19/31, 61.3%) and pleural effusion (11/31, 35.5%). Only one lobe was involved in 11 patients (35.5%). Before diagnosis, 22 patients (71.0%) did not have atypical pathogens in their antimicrobial regimen. After diagnosis, 19 patients (61.3%) received single drug treatment, of which doxycycline or moxifloxacin were the most commonly used drugs. Among 31 patients, three died, nine improved, and nineteen were cured.Conclusion: The clinical manifestations of severe Chlamydia psittaci pneumonia are non-specific. The application of mNGS can improve the diagnostic accuracy of Chlamydia psittaci pneumonia, reduce the unnecessary use of antibiotics, and shorten the course of the disease. Doxycycline-based treatment is effective for severe chlamydia psittaci pneumonia, but it is necessary to understand the secondary bacterial infection and other complications in the course of the disease.
Regulatory network characterization of anthocyanin metabolites in purple sweetpotato via joint transcriptomics and metabolomics
Authors: Xiao Jiping , Xu Xiaoyu , Li Maoxing , Wu Xiaojie , Guo Huachun 
Published: February 2023

IntroductionSweet potato is an important staple food crop in the world and contains abundant secondary metabolites in its underground tuberous roots. The large accumulation of several categories of secondary metabolites result in colorful pigmentation of the roots. Anthocyanin, is a typical flavonoid compound present in purple sweet potatoes and it contributes to the antioxidant activity.MethodsIn this study, we developed joint omics research via by combing the transcriptomic and metabolomic analysis to explore the molecular mechanisms underlying the anthocyanin biosynthesis in purple sweet potato. Four experimental materials with different pigmentation phenotypes, 1143-1 (white root flesh), HS (orange root flesh), Dianziganshu No.88 (DZ88, purple root flesh), and Dianziganshu No.54 (DZ54, dark purple root flesh) were comparably studied.Results and discussionWe identified 38 differentially accumulated pigment metabolites and 1214 differentially expressed genes from a total of 418 metabolites and 50893 genes detected. There were 14 kinds of anthocyanin detected in DZ88 and DZ54, with glycosylated cyanidin and peonidin as the major components. The significantly enhanced expression levels of multiple structural genes involved in the central anthocyanin metabolic network, such as chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase/leucocyanidin oxygenase (ANS), and glutathione S-transferase (GST) were manifested to be the primary reason why the purple sweet potatoes had a much higher accumulation of anthocyanin. Moreover, the competition or redistribution of the intermediate substrates (i.e. dihydrokaempferol and dihydroquercetin) between the downstream production of anthocyanin products and the flavonoid derivatization (i.e. quercetin and kaempferol) under the regulation of the flavonol synthesis (FLS) gene, might play a crucial role in the metabolite flux repartitioning, which further led to the discrepant pigmentary performances in the purple and non-purple materials. Furthermore, the substantial production of chlorogenic acid, another prominent high-value antioxidant, in DZ88 and DZ54 seemed to be an interrelated but independent pathway differentiated from the anthocyanin biosynthesis. Collectively, these data from the transcriptomic and metabolomic analysis of four kinds of sweet potatoes provide insight to understand the molecular mechanisms of the coloring mechanism in purple sweet potatoes.
Whole-genome re-sequencing, diversity analysis, and stress-resistance analysis of 77 grape rootstock genotypes
Authors: Wang Peipei , Zhao Fanggui , Zheng Ting , Liu Zhongjie , Ji Xinglong , Zhang Zhichang , Pervaiz Tariq , Shangguan Lingfei , Fang Jinggui 
Published: February 2023

IntroductionGrape rootstocks play critical role in the development of the grape industry over the globe for their higher adaptability to various environments, and the evaluation of their genetic diversity among grape genotypes is necessary to the conservation and utility of genotypes.MethodsTo analyze the genetic diversity of grape rootstocks for a better understanding multiple resistance traits, whole-genome re-sequencing of 77 common grape rootstock germplasms was conducted in the present study.ResultsAbout 645 billion genome sequencing data were generated from the 77 grape rootstocks at an average depth of ~15.5×, based on which the phylogenic clusters were generated and the domestication of grapevine rootstocks was explored. The results indicated that the 77 rootstocks originated from five ancestral components. Through phylogenetic, principal components, and identity-by-descent (IBD) analyses, these 77 grape rootstocks were assembled into ten groups. It is noticed that the wild resources of V. amurensis and V. davidii, originating from China and being generally considered to have stronger resistance against biotic and abiotic stresses, were sub-divided from the other populations. Further analysis indicated that a high level of linkage disequilibrium was found among the 77 rootstock genotypes, and a total of 2,805,889 single nucleotide polymorphisms (SNPs) were excavated, GWAS analysis among the grape rootstocks located 631, 13, 9, 2, 810, and 44 SNP loci that were responsible to resistances to phylloxera, root-knot nematodes, salt, drought, cold and waterlogging traits.DiscussionThis study generated a significant amount of genomic data from grape rootstocks, thus providing a theoretical basis for further research on the resistance mechanism of grape rootstocks and the breeding of resistant varieties. These findings also reveal that China originated V. amurensis and V. davidii could broaden the genetic background of grapevine rootstocks and be important germplasm used in breeding high stress-resistant grapevine rootstocks.
The effect of anti-frustration ability on academic frustration among Chinese undergraduates: A moderated mediating model
Authors: Wu Minru , Huang Hua , Fu Yuanshu , Zhang Xudong 
Published: February 2023

BackgroundWith the ongoing push to improve the quality of talent in all professions, academic pressure on undergraduates is gradually increasing, leading to students feeling increasingly frustrated by academic stressors. As it becomes more widespread, the resulting issue of academic frustration is attracting public attention.AimThe current study explored the relationship between undergraduate anti-frustration ability (AFA) on their academic frustration (AF), focusing specifically on the roles played by core competence (CC) and coping style (CS) in this relationship.MethodsOur sample comprised 1,500 undergraduate students from universities in China. Data collection made use of the Ability to Anti-Frustration Ability Questionnaire, the Academic Frustration Questionnaire, the Core Competence Questionnaire, and the Simple Coping Style Questionnaire.ResultsThe results showed that: (1) AFA negatively predicted the AF of undergraduates, with CC mediating this relationship and (2) CS had a moderating effect on the relationship between CC and AF. We concluded that students who employ positive CS may be more successful in alleviating their AF to a larger extent, with the mediation of CC.InterpretationThe results revealed the mechanism of AFA on AF, which will help schools consider and guide students’ skills and abilities, both academically and personally.
Translation and cultural adaptation of the HELPS Reading Fluency Program into Brazilian Portuguese: A report of systematic adaptation processes and initial evidence of efficacy
Authors: Martins Maíra Anelli , Begeny John C. , Capellini Simone Aparecida 
Published: February 2023

IntroductionAcross multiples languages, research demonstrates the important relationship between reading fluency and comprehension. Put simply, a fluent reader has greater attention and memory resources to use higher-order functions in reading, resulting in better comprehension of text. Some reading fluency interventions have shown positive results in improving students’ text reading fluency and comprehension; however, this research has predominantly been conducted with English-speaking students. For instance, until this report, a comprehensive search revealed only one prior study that evaluated an intervention strategy designed to improve students’ reading fluency in Brazilian Portuguese and no prior studies evaluated an intervention program with that population of students.MethodsThe main goals of this two-part project were to (a) systematically translate, culturally adapt, and pilot test the Helping Early Literacy with Practice Strategies (HELPS) reading fluency program for use in Brazilian Portuguese (referred to as, HELPS-PB); and (b) conduct a preliminary quasi-experimental study of the HELPS-PB program with 23 students in grades 3 to 5 who needed a reading fluency intervention.Results and DiscussionThis report documents the processes and successful adaptation of existing English- and Spanish-versions of HELPS into a new HELPS-PB program. It also offers preliminary evidence showing that students receiving HELPS-PB significantly improved their text reading fluency comparted to students in a control group. Implications for research, practice, and the adaptation of reading fluency programs into other languages are discussed.
Neural mechanisms of musical structure and tonality, and the effect of musicianship
Authors: Jiang Lei , Zhang Ruiqing , Tao Lily , Zhang Yuxin , Zhou Yongdi , Cai Qing 
Published: February 2023

IntroductionThe neural basis for the processing of musical syntax has previously been examined almost exclusively in classical tonal music, which is characterized by a strictly organized hierarchical structure. Musical syntax may differ in different music genres caused by tonality varieties.MethodsThe present study investigated the neural mechanisms for processing musical syntax across genres varying in tonality – classical, impressionist, and atonal music – and, in addition, examined how musicianship modulates such processing.ResultsResults showed that, first, the dorsal stream, including the bilateral inferior frontal gyrus and superior temporal gyrus, plays a key role in the perception of tonality. Second, right frontotemporal regions were crucial in allowing musicians to outperform non-musicians in musical syntactic processing; musicians also benefit from a cortical-subcortical network including pallidum and cerebellum, suggesting more auditory-motor interaction in musicians than in non-musicians. Third, left pars triangularis carries out online computations independently of tonality and musicianship, whereas right pars triangularis is sensitive to tonality and partly dependent on musicianship. Finally, unlike tonal music, the processing of atonal music could not be differentiated from that of scrambled notes, both behaviorally and neurally, even among musicians.DiscussionThe present study highlights the importance of studying varying music genres and experience levels and provides a better understanding of musical syntax and tonality processing and how such processing is modulated by music experience.
The influence of the COVID-19 pandemic on work connectivity behavior
Authors: Liu Yingyan , Zhang Zaisheng , Zhao Heng , Liu Li 
Published: February 2023

IntroductionBased on event system theory, this study analyzed the influence of the event strength of major public health outside the organization on work connectivity behavior.MethodsThe study collected data from 532 employees on their psychological status and working style during the COVID-19 pandemic through an online questionnaire survey.ResultsThe results show that driven by financial risk perception, female employees are more willing to pay work connectivity behavior than male employees and unmarried employees are more willing to pay work connectivity behavior than married employees. The risk perception of employees aged 28–33 has the greatest impact on workplace behavior. The impact of financial risk perception on behavior of employees without children is much higher than that of employees with children. The influence of financial risk perception and social risk perception on their behavior of employees with master’s degree is much higher than that of health risk perception, but the workplace behavior of employees with doctor’s degree is mainly affected by health risk perception.DiscussionThe novelty of the Corona Virus Disease event has a negative influence on work connectivity duration. The criticality, disruption of the Corona Virus Disease event has a positive influence on work connectivity duration. The criticality of the Corona Virus Disease event has a positive influence on work connectivity frequency. Employees’ social risk perception, financial risk perception and health risk perception has a positive influence on the work connectivity duration and work connectivity frequency.
The global prevalence of autism spectrum disorder: A three-level meta-analysis
Authors: Talantseva Oksana I. , Romanova Raisa S. , Shurdova Ekaterina M. , Dolgorukova Tatiana A. , Sologub Polina S. , Titova Olga S. , Kleeva Daria F. , Grigorenko Elena L. 
Published: February 2023

Autism spectrum disorder (ASD) is one the most disabling developmental disorders, imposing an extremely high economic burden. Obtaining as accurate prevalence estimates as possible is crucial to guide governments in planning policies for identification and intervention for individuals with ASD and their relatives. The precision of prevalence estimates can be heightened by summative analyses of the data collected around the world. To that end, we conducted a three-level mixed-effects meta-analysis. A systematic search of the Web of Science, PubMed, EMBASE, and PsycINFO databases from 2000 up to 13 July 2020 was performed, and reference lists of previous reviews and existing databases of prevalence studies were screened. Overall, 79 studies were included in the analysis of ASD and 59–in the analysis of previously existing relevant diagnoses: 30 for Autistic Disorder (AD), 15 for Asperger Syndrome (AS), and 14 for Atypical Autism (AA) and Pervasive Developmental Disorder - Not Otherwise Specified (PDD-NOS); these research reports covered the period from 1994 to 2019. Pooled prevalence estimates were 0.72% (95% CI = 0.61–0.85) for ASD, 0.25% (95% CI = 0.18–0.33) for AD, 0.13% (95% CI = 0.07–0.20) for AS, and 0.18% (95% CI = 0.10–0.28) for the combined group of AA and PDD-NOS. Estimates were higher (1) for the studies that used records-review surveillance rather than other designs; (2) in North America compared with other geographical regions; and (3) in high-income compared with lower-income countries. The highest prevalence estimates were registered in the USA. There was an increase in autism prevalence estimates over time. The prevalence was also significantly higher for children aged between 6 and 12 years compared to children under the age of 5 and over the age of 13 years.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42019131525, identifier CRD42019131525.