bluuebunny/crossref_metadata_2025_split huggingface dataset:
Building Partnership for Social Innovation in Rural Development: Case Studies in Coastal Villages in Indonesia
Authors: Suryanto J , Rahmayanti AZ , Purwanto P , Nadjib M 
Published: May 2023

Abstract
As an archipelagic country, Indonesia has the potential for marine resources that can guarantee the welfare of the community. Coastal villages face infrastructure limitations that hinder the development of marine resource-based potential businesses. This paper elaborates on the importance of partnership to increase the coastal community’s participation in creating social innovation. It is argued that a solid and collaborative action can boost the acceleration of coastal development among community members. This paper was developed from a qualitative study in several West Java and Gorontalo province’s coastal villages. Data collected from interviews and discussions with coastal village community groups were collected in 2021. The result shows that community partnerships can be proposed to create more social innovation in optimizing marine resources. The result implies that social innovation can be developed by a close partnership between community members and the village government in development planning and its implementation. Community partnerships with the government and private businesses designed in village development programs can encourage social innovation so that marine resources can be managed economically. Furthermore, social innovation can become capital for developing local businesses to increase community marine economic activities’ capacity.
Strategic Behavior of Small-Scale Farmers toward Sustainability of Rice Farming: A study of Entrepreneurial skill to Farming Performance
Authors: Sujarwo  , Suciati Luh Putu , Haryati Novi , Meitasari Deny , Zainuddin Ahmad , Murtisari Rini , Lasitya Daffa Sandi , Irwandi Putra , Putri Rosita Widya , Aulia Balqis Mufti 
Published: May 2023

Abstract
Farm households are the most basic socio-economic and decision-making unit in rural areas. Their livelihood strategies will determine how they exploit natural resources and the efficiency of their use. In general, on-farm and off-farm activities are essential components of livelihood strategies that farmers often adopt to increase their incomes. This research aims to understand the most striking factors related to farmers’ entrepreneurship. The study was conducted with a quantitative approach and the location was in Wuluhan Village, Jember Regency Indonesia. Consisting of 50 respondents, this research used Structural Equation Modeling based on WARP-PLS 7.0 to analyze the prominent traits that lead to the best farming performance. While the findings show that most of the variables were significant, it can be clearly seen that initiative was the best trait that describes the farmers in the research area. Thus, in the future, dynamics, leadership and collective bargaining can be developed more in shaping their readiness to face the challenges in sustainable rice farming.
The Women’s Role in the Resilience of Farmers’ Households during the COVID-19 Pandemic in Gubugklakah Village, Poncokusumo District, Malang Regency
Authors: Hidayat K , Hanim F 
Published: May 2023

Abstract
The cause of rural changes, in terms of demographic, technological developments, climate changes, and the Covid-19 pandemic potential to cause vulnerabilities, especially for women as individuals in household members. These must be responded with livelihood resilience by involving the women’s role to contribute in the agricultural and non-agricultural sectors. This study aims to (1) describe the vulnerabilities of farmers’ households and (2) analyze women’s role in household resilience through the use of livelihood assets during the Covid-19 pandemic. This research was conducted in Gubugklakah village, Malang regency as a tourist village affected by the closure of TNBTS tourist visits due to the Covid-19 pandemic. This research used the simple random sampling technique, with total sample of 64 women farmers. Data were analyzed using WarpPLS software. The results showed that farmers’ households experienced several vulnerabilities by that the households’ livelihood assets: natural, physic, human, social and financial capital can be optimized to achieve a degree of resilience. The women’s role in resilience efforts is as the core of the household, because all financial cycles involve housewives’ role, such as reducing consumption expenditures, selling jewelry assets, taking savings, involving in farm worker, and others.
Cholesterol Contributes to Risk, Severity, and Machine Learning-Driven Diagnosis of Lyme Disease
Authors: Forrest Iain S , O’Neal Anya J , Pedra Joao H F , Do Ron 
Published: May 2023

AbstractBackgroundLyme disease is the most prevalent vector-borne disease in the US, yet its host factors are poorly understood and diagnostic tests are limited. We evaluated patients in a large health system to uncover cholesterol's role in the susceptibility, severity, and machine learning–based diagnosis of Lyme disease.MethodsA longitudinal health system cohort comprised 1 019 175 individuals with electronic health record data and 50 329 with linked genetic data. Associations of blood cholesterol level, cholesterol genetic scores comprising common genetic variants, and burden of rare loss-of-function (LoF) variants in cholesterol metabolism genes with Lyme disease were investigated. A portable machine learning model was constructed and tested to predict Lyme disease using routine lipid and clinical measurements.ResultsThere were 3832 cases of Lyme disease. Increasing cholesterol was associated with greater risk of Lyme disease and hypercholesterolemia was more prevalent in Lyme disease cases than in controls. Cholesterol genetic scores and rare LoF variants in CD36 and LDLR were associated with Lyme disease risk. Serological profiling of cases revealed parallel trajectories of rising cholesterol and immunoglobulin levels over the disease course, including marked increases in individuals with LoF variants and high cholesterol genetic scores. The machine learning model predicted Lyme disease solely using routine lipid panel, blood count, and metabolic measurements.ConclusionsThese results demonstrate the value of large-scale genetic and clinical data to reveal host factors underlying infectious disease biology, risk, and prognosis and the potential for their clinical translation to machine learning diagnostics that do not need specialized assays.
Single High Dose of Liposomal Amphotericin B in Human Immunodeficiency Virus/AIDS-Related Disseminated Histoplasmosis: A Randomized Trial
Authors: Pasqualotto Alessandro C , Lana Daiane Dalla , Godoy Cassia S M , Leitão Terezinha do Menino Jesus Silva , Bay Monica B , Damasceno Lisandra Serra , Soares Renata B A , Kist Roger , Silva Larissa R , Wiltgen Denusa , Melo Marineide , Guimarães Taiguara F , Guimarães Marilia R , Vechi Hareton T , de Mesquita Jacó R L , Monteiro Gloria Regina de G , Adenis Antoine , Bahr Nathan C , Spec Andrej , Boulware David R , Israelski Dennis , Chiller Tom , Falci Diego R 
Published: May 2023

Abstract

Background
Histoplasmosis is a major AIDS-defining illness in Latin America. Liposomal amphotericin B (L-AmB) is the drug of choice for treatment, but access is restricted due to the high drug and hospitalization costs of the conventional long regimens.

Methods
Prospective randomized multicenter open-label trial of 1- or 2-dose induction therapy with L-AmB versus control for disseminated histoplasmosis in AIDS, followed by oral itraconazole therapy. We randomized subjects to: (i) single dose 10 mg/kg of L-AmB; (ii) 10 mg/kg of L-AmB on D1, and 5 mg/kg of L-AmB on D3; (iii) 3 mg/kg of L-AmB daily for 2 weeks (control). The primary outcome was clinical response (resolution of fever and signs/symptoms attributable to histoplasmosis) at day 14.

Results
A total of 118 subjects were randomized, and median CD4+ counts, and clinical presentations were similar between arms. Infusion-related toxicity, kidney toxicity at multiple time-points, and frequency of anemia, hypokalemia, hypomagnesemia, and liver toxicity were similar. Day 14 clinical response was 84% for single-dose L-AmB, 69% 2-dose L-AmB, and 74% for control arm (P = .69). Overall survival on D14 was 89.0% (34/38) for single-dose L-AmB, 78.0% (29/37) for 2-dose L-AmB, and 92.1% (35/38) for control arm (P = .82).

Conclusions
One day induction therapy with 10 mg/kg of L-AmB in AIDS-related histoplasmosis was safe. Although clinical response may be non-inferior to standard L-AmB therapy, a confirmatory phase III clinical trial is needed. A single induction dose would markedly reduce drug-acquisition costs (&gt;4-fold) and markedly shorten and simplify treatment, which are key points in terms of increased access.
Utilizing Mass Spectrometry to Detect and Isotype Monoclonal Proteins in Urine: Comparison to Electrophoretic Methods
Authors: Moonen Danelle H , Kohlhagen Mindy , Dasari Surendra , Willrich Maria A , Kourelis Taxiarchis , Dispenzieri Angela , Murray David L 
Published: May 2023

Abstract

Background
Matrix assisted laser desorption ionization time of flight mass spectrometry coupled to immune enrichment (MASS-FIX) as an alternative to serum immunofixation electrophoresis has demonstrated increased sensitivity in monoclonal protein (MP) detection with improved laboratory workflow. This study explored similar replacement of urine immunofixation electrophoresis (u-IFE) with urine MASS-FIX (u-MASS-FIX) by method comparison.

Methods
Residual urine (n = 1008) from Mayo Clinic patients with a known plasma cell disease were assayed neat by u-MASS-FIX analysis. Each sample was paired with the following: u-IFE, urine total protein, urine protein electrophoresis, serum κ/λ free light chain (LC) ratio (rFLC), and serum MASS-FIX (s-MASS-FIX). Analytical sensitivities were measured in pooled urine spiked with daratumumab.

Results
u-IFE and u-MASS-FIX had 91% agreement in determining the presence/absence of MPs (Cohen kappa = 0.8200). In discrepant cases, serum rFLC statistically aligned more closely with positive u-MASS-FIX cases than u-IFE. Patients positive by both s-MASS-FIX and u-MASS-FIX had matching MP masses (±20 daltons) in 94% of cases. The u-MASS-FIX spectra further identified κ/λ LC fragments and glycosylated LCs not appreciated on u-IFE. The unconcentrated u-MASS-FIX limit of detection of 0.156 mg/mL was determined equivalent to 100× concentrated u-IFE.

Conclusion
u-MASS-FIX is a reliable alternative to u-IFE with the added benefits of LC glycosylation detection and MP mass tracking between serum and urine. Furthermore, u-MASS-FIX is performed using neat urine. Eliminating the need to concentrate urine for u-IFE has potential to increase productivity by decreasing labor minutes per test.
Driving Under the Influence of Cannabis: Impact of Combining Toxicology Testing with Field Sobriety Tests
Authors: Fitzgerald Robert L , Umlauf Anya , Hubbard Jacqueline A , Hoffman Melissa A , Sobolesky Philip M , Ellis Shannon E , Grelotti David J , Suhandynata Raymond T , Huestis Marilyn A , Grant Igor , Marcotte Thomas D 
Published: May 2023

Abstract

Background
Cannabis is increasingly used both medically and recreationally. With widespread use, there is growing concern about how to identify cannabis-impaired drivers.

Methods
A placebo-controlled randomized double-blinded protocol was conducted to study the effects of cannabis on driving performance. One hundred ninety-one participants were randomized to smoke ad libitum a cannabis cigarette containing placebo or delta-9-tetrahydrocannabinol (THC) (5.9% or 13.4%). Blood, oral fluid (OF), and breath samples were collected along with longitudinal driving performance on a simulator (standard deviation of lateral position [SDLP] and car following [coherence]) over a 5-hour period. Law enforcement officers performed field sobriety tests (FSTs) to determine if participants were impaired.

Results
There was no relationship between THC concentrations measured in blood, OF, or breath and SDLP or coherence at any of the timepoints studied (P &gt; 0.05). FSTs were significant (P &lt; 0.05) for classifying participants into the THC group vs the placebo group up to 188 minutes after smoking. Seventy-one minutes after smoking, FSTs classified 81% of the participants who received active drug as being impaired. However, 49% of participants who smoked placebo (controls) were also deemed impaired at this same timepoint. Combining a 2 ng/mL THC cutoff in OF with positive findings on FSTs reduced the number of controls classified as impaired to zero, 86 minutes after smoking the placebo.

Conclusions
Requiring a positive toxicology result in addition to the FST observations substantially improved the classification accuracy regarding possible driving under the influence of THC by decreasing the percentage of controls classified as impaired.
Image-based ring size prediction for mitral valve repair
Authors: Akansel Serdar , Kofler Markus , Van Praet Karel M , Sündermann Simon H , Kukucka Marian , Jacobs Stephan , Falk Volkmar , Kempfert Jörg 
Published: May 2023

Abstract

OBJECTIVES
Annuloplasty rings are routinely used in mitral valve repair (MVr). However, accurate annuloplasty ring size selection is essential to obtain a favourable outcome. Moreover, ring sizing can be challenging in some patients and is highly influenced by surgeons' experience. This study investigated the utility of three-dimensional mitral valve (3D-MV) reconstruction models to predict annuloplasty ring size for MVr.

METHODS
A total of 150 patients undergoing minimally invasive MVr with annuloplasty ring due to Carpentier type II pathology and who were discharged with none/trace residual mitral regurgitation were included. 3D-MV reconstruction models were created with a semi-automated software package (4D MV Analysis) to quantitate mitral valve geometry. To predict the ring size, univariable and multivariable linear regression analyses were performed.

RESULTS
Between 3D-MV reconstruction values and implanted ring sizes, the highest correlation coefficients were provided by commissural width (CW) (0.839; P &lt; 0.001), intertrigonal distance (ITD) (0.796; P &lt; 0.001), annulus area (0.782; P &lt; 0.001), anterior mitral leaflet area (0.767; P &lt; 0.001), anterior–posterior diameter (0.679; P &lt; 0.001) and anterior mitral leaflet length (0.515; P &lt; 0.001). In multivariable regression analysis, only CW and ITD were found to be independent predictors of annuloplasty ring size (R2 = 0.743; P &lt; 0.001). The highest level of agreement was achieved with CW and ITD, and 76.6% of patients received a ring with no &gt;1 ring size difference from the predicted ring sizes.

CONCLUSIONS
3D-MV reconstruction models can support surgeons in the decision-making process for annuloplasty ring sizing. The present study may be a first step towards accurate annuloplasty ring size prediction using multimodal machine learning decision support.
Racial bias in media coverage: accounting for structural position and public interest
Authors: Shor Eran , van de Rijt Arnout 
Published: May 2023

Abstract
Is media coverage racially biased? Past studies documenting differences in the quantity of coverage are small scale or anecdotal. In this article, we investigate whether Blacks receive less coverage than Whites who have reached similarly prominent positions and enjoy similar public interest. We analysed 200 million newspaper references in English-language media to about 32,000 prominent Black and White individuals, predominantly US born. The results do not support the bias hypothesis: Blacks overall receive systematically more coverage than Whites in comparable structural positions and their coverage is on par with that of select Whites who attract equal public interest.
The social amoeba Dictyostelium discoideum rescues Paraburkholderia hayleyella, but not P. agricolaris, from interspecific competition
Authors: Medina James M , Queller David C , Strassmann Joan E , Garcia Justine R 
Published: May 2023

Abstract
Bacterial endosymbionts can provide benefits for their eukaryotic hosts, but it is often unclear if endosymbionts benefit from these relationships. The social amoeba Dictyostelium discoideum associates with three species of Paraburkholderia endosymbionts, including P. agricolaris and P. hayleyella. These endosymbionts can be costly to the host but are beneficial in certain contexts because they allow D. discoideum to carry prey bacteria through the dispersal stage. In experiments where no other species are present, P. hayleyella benefits from D. discoideum while P. agricolaris does not. However, the presence of other species may influence this symbiosis. We tested if P. agricolaris and P. hayleyella benefit from D. discoideum in the context of resource competition with Klebsiella pneumoniae, the typical laboratory prey of D. discoideum. Without D. discoideum, K. pneumoniae depressed the growth of both Paraburkholderia symbionts, consistent with competition. P. hayleyella was more harmed by interspecific competition than P. agricolaris. We found that P. hayleyella was rescued from competition by D. discoideum, while P. agricolaris was not. This may be because P. hayleyella is more specialized as an endosymbiont; it has a highly reduced genome compared to P. agricolaris and may have lost genes relevant for resource competition outside of its host.
Transcription factor fluctuations underlie cell-to-cell variability in a signaling pathway response
Authors: Ramu Avinash , Cohen Barak A 
Published: May 2023

Abstract
Stochastic differences among clonal cells can initiate cell fate decisions in development or cause cell-to-cell differences in the responses to drugs or extracellular ligands. One hypothesis is that some of this phenotypic variability is caused by stochastic fluctuations in the activities of transcription factors (TFs). We tested this hypothesis in NIH3T3-CG cells using the response to Hedgehog signaling as a model cellular response. Here, we present evidence for the existence of distinct fast- and slow-responding substates in NIH3T3-CG cells. These two substates have distinct expression profiles, and fluctuations in the Prrx1 TF underlie some of the differences in expression and responsiveness between fast and slow cells. Our results show that fluctuations in TFs can contribute to cell-to-cell differences in Hedgehog signaling.
eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment
Authors: He X , Jeffery N W , Stanley R R E , Hamilton L C , Rubidge E M , Abbott C L 
Published: May 2023

Abstract
Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.
APOBEC3F Is a Mutational Driver of the Human Monkeypox Virus Identified in the 2022 Outbreak
Authors: Suspène Rodolphe , Raymond Kyle A , Boutin Laetitia , Guillier Sophie , Lemoine Frédéric , Ferraris Olivier , Tournier Jean-Nicolas , Iseni Frédéric , Simon-Lorière Etienne , Vartanian Jean-Pierre 
Published: May 2023

Abstract

Background
On May 6, 2022, a powerful outbreak of monkeypox virus (MPXV) had been reported outside of Africa, with many continuing new cases being reported around the world. Analysis of mutations among the 2 different lineages present in the 2021 and 2022 outbreaks revealed the presence of G-&gt;A mutations occurring in the 5′GpA context, indicative of APOBEC3 cytidine deaminase activity.

Methods
By using a sensitive polymerase chain reaction (differential DNA denaturation PCR) method allowing differential amplification of AT-rich DNA, we analyzed the level of APOBEC3-induced MPXV editing in infected cells and in patients.

Results
We demonstrate that G-&gt;A hypermutated MPXV genomes can be recovered experimentally from APOBEC3 transfection followed by MPXV infection. Here, among the 7 human APOBEC3 cytidine deaminases (A3A-A3C, A3DE, A3F–A3H), only APOBEC3F was capable of extensively deaminating cytidine residues in MPXV genomes. Hyperedited genomes were also recovered in ∼42% of analyzed patients. Moreover, we demonstrate that substantial repair of these mutations occurs. Upon selection, corrected G-&gt;A mutations escaping drift loss contribute to the MPXV evolution observed in the current epidemic.

Conclusions
Stochastic or transient overexpression of the APOBEC3F gene exposes the MPXV genome to a broad spectrum of mutations that may be modeling the mutational landscape after multiple cycles of viral replication.
(Dis)possessive Borders, (Dis)possessed Bodies: Race and Property at the Postcolonial European Borders
Authors: Brito Tarsis 
Published: May 2023

Abstract
There has been a profusion of institutionalized practices of confiscation and destruction of migrants’ belongings during European bordering operations conducted by the police and border authorities. Clothes, shoes, money, food, mobile phones, and even water have been among the items seized by authorities, a practice that exposes migrants to multiple risks. That said, despite the pervasiveness of current (dis)possessive methods, scholars have not yet sufficiently theorized the historical and current links between property, race, and borders. This article argues that such (dis)possessive practices at Europe's borders are not simply another method of governance that emerges at Europe's borderzones. Rather, (dis)possession is seen here as central to the very (post)colonial functioning of the border itself. The argument is, on the one hand, that Europe's borders have been embedded within a (post)colonial and racial capitalist global order predicated upon multifaceted forms of (dis)possession. And, on the other hand, it is claimed that borders themselves have been sites of continual forms of colonial and racial (dis)possession. In so doing, the article shows how (dis)possession has historically allowed Europe to demarcate, reinforce, and police the status of racialized bodies as less than human and property-like, that is, as bodies available for colonial and capitalist consumption.