bluuebunny/crossref_metadata_2025_split huggingface dataset:
Separation of Conjoined Twins Utilizing Cardiopulmonary Bypass
Authors: Banchieri Candace L. , Thompson Steven J. , Rivard Dennis C. 
Published: August 2023

The incidence of conjoined twins occurs once in every two million live births. Successful separation has occurred in 50% of these patients. Previous attempts to separate twins at the sagittal sinus have resulted in death. On September 5, 1987, the team at Johns Hopkins Hospital was the first to successfully separate conjoined twins at the sagittal sinus. One key to this success was the use of cardiopulmonary bypass, deep hypothermia and circulatory arrest.
Each patient was cannulated with a 14 Fr. aortic cannula and a 22 Fr. venous cannula in the right atrium. They were then connected to completely separate cardiopulmonary bypass circuits. Cardiopulmonary bypass was initiated utilizing a roller pump, pediatric membrane oxygenator with integral cardiotomy, and an arterial filter. The twins were cooled to 20 degrees centigrade in preparation for circulatory arrest. During circulatory arrest, separation occurred and repair was accomplished utilizing direct suture and pericardial patches. When repair was complete, cardiopulmonary bypass was resumed and rewarming begun. At a rectal temperature of 33 degrees centigrade, cardiopulmonary bypass was discontinued.
The two major benefits of extra-corporeal circulation in this procedure were maintaining hemodynamic stability and the ability to regulate metabolic demands. The use of cardiopulmonary bypass was a solution to the obstacles presented. The ability to control blood flow, temperature and volume resulted in a successful separation of these patients.
The Biomedical Technology/Information Explosion: Find Yourself A Foxhole
Authors: Tyndal Charles M. , Berryessa Richard G. 
Published: August 2023

Approximately 5500 biomedical papers are published every day and the half-life of this information is currently only 3-4 years. Therefore, it is not only important but nearly impossible to keep up-to-date on the literature relating to one's field.
This paper will propose a Journal Club (JC) format designed for perfusionists. Our experience with JC the last three years encourages us to share what we have learned about design, scheduling, benefits, pitfalls, and the evolution of a JC. We will suggest a list of 50+ journals that represent a cross section of the literature from disciplines affecting perfusionists. We will discuss techniques for surveying the literature, reporting at JC, recording participation, and creating a database for reference. JC is the best way to stay current with new information in our field and the benefits justify the effort required.
Stereoselective Synthesis of Highly Functionalized Aminobenzothiazole-Fused Spirooxindole Derivatives: in silico and in vitro Anti-Diabetic Studies
Authors: Velmathi Sivan , Nivetha Narayanasamy , Patil Shashank M. , Ramu Ramith , Sreenivasa Swamy 
Published: August 2023

AbstractA series of highly functionalized spirooxindole pyrro­lizidine/pyrrolothiazole derivatives have been synthesized by the three-component 1,3-dipolar cycloaddition reaction of benzothiazolyl amides with isatin-based azomethine ylides. The pharmacologically significant spirooxindole derivatives bearing one quaternary carbon and four stereocenters were obtained in excellent yields (up to 93%). The compounds were screened for their anti-diabetic activity against two enzymes, α-glucosidase and α-amylase. The results exhibited potent inhibitory activity against these enzymes, especially N-(benzo[d]thiazol-2-yl)-5-fluoro-2-oxo-7′-phenyl-1′,6′,7′,7a′-tetrahydro-3′H-spiro[indoline-3,5′-pyrrolo[1,2-c]thiazole]-6′-carboxamide (6b), which showed excellent activity compared to the standard acarbose. Molecular docking against the receptors showed excellent interactions of the synthesized compounds in a similar way to acarbose. Further, the docking results of the spirooxindole pyrrolothiazole (6b) evinced the strong binding interactions of the compound with the receptors. Additionally, molecular dynamics simulations were carried out and confirmed the stability of the spirooxindole pyrrolothiazole (6b) in the active pockets of enzymes over 100 ns.
Bibliometric Analysis of Scientific Output Growth in the Field of Lung Transplantation
Authors: Tokur Murat Emre , Alkan Sevil 
Published: August 2023

Abstract
Background Lung transplantation (LT) has recently emerged as a scientifically validated curative therapeutic modality for patients afflicted with end-stage lung disease. This study aimed to conduct a global bibliometric analysis of research articles on LT between 1983 and 2021.
Methods Employing the Web of Science database, a bibliometric analysis was conducted to assess the expansion of scientific output within the field of LT. We searched specific bibliometric characteristics such as language, and year of publication, first author, institutional affiliation, main publishing journals, and highly cited articles. Additionally, we made comparisons of the most productive countries. The VOSviewer program and the open-source visualization software Biblioshiny (version 2.0) were used to perform the bibliometric analysis.
Results We identified 10,467 articles on LT published between 1983 and 2021, of which 94.898% were published in the Science Citation Index Expanded. The articles were from 101 different research areas. The publications were from 81 different countries globally, and mostly from the United States (41.196%), Germany (7.118%), and Canada (6.372%). The Journal of Heart and Lung Transplantation was the most published journal. Four thousand seven hundred and ninety three of the publications were published in the last 10 years with a 78,781 citation number in total. The highest number of publications and citations was in 2021.
Conclusion The majority of cutting-edge research findings are focused on only a few developed nations, and exchanges with emerging nations are still in their infancy. The United States has a strong, commanding position among the active countries in LT.
Preoperative Preparatory Talk (PPT): Developing Operative Expertise of Plastic Surgery Trainees by Three Rounds of Preceptor-Trainee Discussions
Authors: Vathulya Madhubari , Pasricha Arush , Mohapatra Devi Prasad , Jayaprakash Praveen A. 
Published: August 2023

Abstract
Background Plastic surgery training requires the trainee to assist in surgeries to improve their on-table decision making and hone their surgical skills, but this results in an increased risk of intraoperative complications and increased operative time. It is important to have a training method that orients the trainee toward the surgery to ensure patient safety.
Materials and Methods A training method called preoperative preparatory talk (PPT) was devised in which the preceptor orients the trainee toward the planned surgery in three phases. Comparison and statistical analysis of mean operative times of four stages of free flap surgeries after PPT and without PPT were done. Objective Structured Assessment of Technical Skill (OSATS) scores of surgical trainees were also documented for surgeries done with and without PPT and statistical analysis was done for comparing these scores.
Results Statistical analysis via unpaired t-test confirmed that after applying PPT, there was a significant decrease in time taken in three out of four stages of free flap surgeries: flap planning and harvesting, recipient site preparation and vessel dissection, and flap division and partial inset. Trainees were found to be better oriented toward the surgery which resulted in a better performance on table that was confirmed by statistical analysis of OSATS score via unpaired t-test.
Conclusion PPT ensures better learning for the resident and improves patient safety because of better orientation of the operating team toward the procedure and operating steps. This reduces the operative time of free flap surgeries. We recommend this training method to be incorporated in plastic surgery training programs.
Glycated Albumin and Glycemia in Pregnancy and Postpartum: A Pilot Study
Authors: Soffer Marti D. , James Kaitlyn E. , Thaweethai Tanayott , Callahan Michael , Barth William H. , Powe Camille E. 
Published: August 2023

Objective Percent glycated albumin (%GAlb) is a marker of glycemia over the past 2 to 3 weeks in nonpregnant individuals. Longitudinal changes in %GAlb extending throughout pregnancy and postpartum (PP) have not been described. We aimed to describe levels of %GAlb throughout pregnancy and PP and relationships with glycemia.
Study Design Fifty women among those in the Study of Pregnancy Regulation of INsulin and Glucose cohort underwent 75-g oral glucose tolerance tests (OGTTs) at a mean of 13 weeks (V1) and 26 weeks (V2) of gestation and 11 weeks' PP. %GAlb was measured on frozen plasma samples.
Results Total albumin decreased from V1 to V2 and increased PP to levels higher than at V1. %GAlb declined between V1 and V2 (β = − 0.63% 95% CI [−0.8, −0.6] p < 0.001) and remained stable between V2 and PP (β = − 0.04% [−0.3, 0.2] p = 0.78). Body mass index (BMI) was inversely related to %GAlb in pregnancy (V1: rho = − 0.5, p = 0.0001; V2 rho = − 0.4, p = 0.006), but not PP (rho = − 0.15, p = 0.31). The longitudinal changes in %GAlb persisted after adjusting for BMI. Neither glycemia measurements nor hemoglobin A1c were associated with %GAlb at any time point, and adjustments for BMI did not reveal additional associations.
Conclusion %GAlb decreases between early and late gestation and remains decreased PP, despite a PP increase in total albumin above early pregnancy values. Given the lack of correlation with OGTT values or A1c, %GAlb is unlikely to be useful in assessing glycemia in pregnant or PP women.
Key Points
An Account of a Giant Adolescent Vulvar Lipoma
Authors: Vats Garima , DCunha Aureen , Rai Sandeep B. , Raghuraj U. 
Published: August 2023

AbstractThough they are commonly known as “universal tumors,” lipomas are rarely found in the vulva. In this report, we discuss the case of a 15-year-old girl who presented with an enormous (26.5 × 21.5 × 6.5 cm) left labial swelling that had been progressively enlarging for a year and had led to extensive distortion of the vulvar anatomy. Following imaging, surgical excision with vulvar reconstruction was performed focusing on esthesis and function. Pathological examination confirmed the clinical and radiological diagnosis of a lipoma. The patient is doing clinically well at 1 year of follow-up with significant improvement in her academic performance and self-esteem.
Closure of Nasal Septal Perforations Using a Diced Cartilage in Fascia Graft
Authors: Spatz Cornelia , Kühnel Thomas , Stegmann Achim , Schwan Franziska , Bumm Klaus , Bohr Christopher 
Published: August 2023

AbstractThe spectrum of surgical techniques in the repair of nasal septal defects is wide. The objective of this study was to assess the feasibility of using a diced cartilage in fascia (DC-F) graft for successful closure of nasal septal perforations and to evaluate symptom reduction. This was a retrospective study of 18 patients undergoing surgical repair of symptomatic nasoseptal perforations of different etiologies using a DC-F graft from 2020 until 2021. The procedure was feasible in all of the 18 patients. Reconstruction of septal defects with a DC-F graft led to reduction of crust formation, reduction of epistaxis, and improvement of nasal breathing in 13 out of the 18 patients when seen for their 2-month follow-up. Reperforation occurred in three cases, leaving defects of 1, 7, and 5 mm in diameter. In one case, the reperforation was symptomatic. A DC-F graft proved to be a reliable and reproducible method for the closure of nasoseptal perforations of variable sizes, of different locations, and of different etiologies.
The Effect of Immersion Duration of java barb larvae (Barbornymus gonionotus) in the combination of thyroxine hormone and recombinant growth hormone (rGH) on the egg yolk absorption and growth
Authors: Yuniarti T , Hastuti S , Nugroho R A , Siswandha S A 
Published: August 2023

Abstract
Java barb (Tawes) is one of the endemic freshwater fish in Indonesia which has being widely cultivated. The time rearing of Java barb from larva to market size is generally 5-6 months. Egg yolk absorption rate and growth of Java barb fish can be increased by hormonal manipulation using thyroxine hormone and recombinant growth hormone (rGH). The length of immersion time affects the performance of hormones in the fish’s body. The purpose of this study is to determine the effect of immersion duration and the best immersion time in a combination of thyroxine and rGH on the egg yolk absorption and the growth of Java barb larvae. This research was conducted on 23 August-1 November 2021 at Laboratorium Pengujian Kesehatan Ikan dan Lingkungan (LPKIL) in Muntilan, Magelang regency. The testing fishes used were early hatched Java barb fish larvae that still had egg yolks. The method used was a completely random design (CRD) with 4 treatments and 3 replications and each was done with a combination of 0.1 mg/l thyroxine hormone and 2.5 mg/l rGH with treatments A (control), B (1 hour immersion time), C (2 hours immersion time) and D (3 hours immersion time). The results showed that the effect of immersion duration in combination of thyroxine and rGH had a significant effect on the rate of egg yolk absorption and the growth of Java barb fish larvae. The best immersion time was treatment D (3 hours immersion duration) which resulted in 4%/hour of the absorption rate of egg yolk absorption rate, 12.53±0.15 mm absolute length growth, 0.047±0.002 g absolute weight growth, 13.82±0.15 % of specific growth rate, and 54.67±4.62 % survival rate.
Characteristics of octopus fishery in Ampana Tojo Una-Una Sea waters
Authors: Noegroho T , Kembaren D D , Nurdin E , Panggabean A S , Taufik M , Fauzi M , Wibowo S 
Published: August 2023

Abstract
Octopus fishing activity have been established in Ampana Waters and surrounding areas since the 1990s. The goal of this research is to examine the octopus fishery in Ampana waters and its surroundings. The research methodology used in this study are CPUE and Length-based assessment which includes some analysis of catch per unit effort, fishing season, size structure, weigh distribution, length-weight relationship, fishing ground, fishing gear operation and specification, and the marketing flow. CPUE ranges from 7–15 kg/units/day, with an average of 11 kg/units/day; the fishing season runs from August to November; mantle length ranges from 8–22 cmML, and the length mode is 14 cmML; male octopus length ranges from 9–19 cmML, while the female ranges from 8–22 cmML; weight ranges from 300–2500 gr, with the male dominant at 800 gr and the female dominant at 1000 gr. Growth patterns for both males and females are negative allometric. Ampana fishermen fished around Tanjung Api, while Pagimana fishermen fished north of Bunta beach. Fishing equipment used included an octopus fishing rod, hook, and arrow, as well as aggregating devices such as gara-gara and a crustacean-like octopus fishing rod. The fishermen sold their catch to the first collector, who then delivered it to octopus middlemen in Ampana, Pagimana, and Luwuk before transporting it to Makassar.
Post-Catch Handling Affects the Quality of Skipjack Tuna (Katsuwonus pelamis)
Authors: Litaay C , Opier RDA , Horhoruw SM , Hehuwat J , Arfah H , Rugebregt MJ 
Published: August 2023

Abstract
Skipjack tuna is a fishery product that has important economic value and high nutritional value but is easily damaged and rotten. This study aims to determine the post-catch handling of skipjack tuna, from onboard to the fish landing site. This research was an experimental study with quality test parameters including pH value, sensory analysis, and temperature for 12 hrs from the time on the board until the fish landing centre. The results showed that the post-capture handling of skipjack tuna with the 1:1 and 1:2 cooling methods was on the same good freshness level. Treatment methods ice and fish with a ratio of 1:1 was better than ice and fish with a ratio of 1:2 based on pH value, sensory analysis, and temperature.
Mangrove damage based on canopy density derived on SPOT 6 at Ngurah Rai forest park, Bali
Authors: Anggraini N , Suardana A A M A P , Nandika M R , Ulfa A , Ginting D N B , Adawiah S W , Mukhoriyah  , Dewi E K , Setiawan K T , Marpaung S 
Published: August 2023

Abstract
The Indonesian government has launched a mangrove rehabilitation program of 600,000 ha in 2021-2024. This program needs information about mangrove forest damage obtained quickly and accurately. Remote sensing technology is a solution to the availability of the required speed of information. Mangrove damage information can be obtained from the canopy density derived from the vegetation index. This study aims to detect mangrove damage based on the density level derived from the vegetation index. The data used are SPOT 6 satellite imagery recorded in 2020 with the research location in Ngurah Rai forest park. The vegetation index used is Normalized Different Vegetation Index (NDVI), Green Normalized Different Vegetation Index (GNDVI), and Optimized Soil Adjusted Vegetation Index (OSAVI). Field data for validation were collected by the vertical photos that provide information on the percentage of canopy density. Furthermore, the density model was built using linear regression and validation using the coefficient of determination (R2). The results show that the vegetation index positively correlates with canopy density. NDVI and OSAVI have an identical R2 of 0.75, while GNDVI has an R2 of 0.70. Generally, the mangroves in Ngurah Rai forest park are dominated by mangroves in good condition and only 2.81% in some locations in damaged condition.
Ship detection using Google Earth Pro and SAR Sentinel-1 Satellite in Karimunjawa, Indonesia
Authors: Satya Eridhani Dharma , Sabdono Agus , Wijayanti Diah Permata , Helmi Muhammad , Widiaratih Rikha , Satriadi Alfi , Puryajati Ardiansyah Desmont 
Published: August 2023

Abstract
Karimunjawa is one of the tourist icon due to the beauty of its coral reefs. Many tourists visit for snorkeling and diving in Karimunjawa which requires ships for transportation. This study aims to determine the distribution of ships in Karimunjawa before and during the COVID-19 pandemic using the Google Earth Pro satellite and Synthetic Aperture Radar (SAR) Sentinel-1. There were 6 islands selected to represent the anchorage level based on the number of tourist arrivals. The results of this study indicate that the Sentinel-1 SAR has high capability in detecting ships by using the Constant False Alarm Rate (CFAR) algorithm method, which was detected with a total of 83 ships on 31st March 2021 in six island representatives. This funding is supported by SAR images that have a high spatial resolution of 5m, moreover, ships are typically constructed from large flat metal sheets and hence are usually radar bright and detectable in SAR imagery. Even though Google Earth Pro has a higher resolution of 30 cm, the ship detection process is identified manually. This study showed the Covid-19 pandemic condition had a significant decreasing effect on the number of ships detected, there was a 41.6% decrease from 2019 to 2020 and a 41.95% decrease from 2020 to 2021 due to the temporary closure of Karimunjawa.
Sensory Value, Proximate Analysis, Amino Acid and Fatty Acid Profile of Steamed and Boiled Blue Swimming Crab (Portunus pelagicus)
Authors: Suhaeli Fahmi A , Agustini Tri Winarni , Jayanto Bogi Budi , Prihantoko Kukuh Eko 
Published: August 2023

Abstract
Whole blue swimming crab (Portunus pelagicus) is usually steamed or boiled before taking the meat for canning. The choice of the cooking process between steaming or boiling can determine the characteristics of blue swimming crab (Portunus pelagicus) meat. This study aims to determine and compare the flavor profile of blue swimming crab cooked by steaming and boiling. Whole blue swimming crab were steamed and boiled for 15 minutes at 100°C respectively. The cooked blue swimming crab meat was then tested for yield, sensory value, proximate analysis, amino acid and fatty acid profile. The results showed that the steaming process gave higher yields and sensory value than boiled blue swimming crab.
Antioxidant Activity of Gelatin Hydrolysate from Nile Tilapia (Oreochromis niloticus) Skin by Hydrolysis Using Flavorzyme
Authors: Artwiastia A , Riyadi P H , Anggo A D , Arifin M H 
Published: August 2023

Abstract
Nile tilapia skin is one of the wastes produced by the tilapia fillet industry that contains quite high protein. One form of Nile tilapia skin utilization that is very potential is gelatin, which is known to have bioactive potential when hydrolyzed properly. The choice of Flavorzyme as a hydrolyzing agent is because it can hydrolyze proteins specifically and does not cause damage. The result of gelatin hydrolysis by flavorzyme at 50°C was the gelatin hydrolysate liquid product. The purposes of this research were to determine the effect of different concentrations of Flavorzyme on the antioxidant activity of Nile tilapia skin gelatin hydrolysate and the best enzyme concentration on Nile tilapia skin gelatin hydrolysate. This research method used a completely randomized design with different enzyme concentrations (0%, 0.5%, 1% and 1.5%). The data obtained were tested by normality test, homogeneity test, ANOVA test, and followed by the honest significant different test to find out the real difference in treatment. The results of data analysis showed that the addition of different concentrations of the flavorzyme had a significantly different effect (P<5%) on the degree of hydrolysis, protein content, and DPPH value. The best Nile tilapia skin gelatin hydrolysate was the product with addition of 1% flavorzyme enzyme concentration with a hydrolysis degree value of 51.52±3.66%, protein content of 8.46±0.24%, and an antioxidant activity value of 31.97. ±1.88%.
Optimization of Enzymatic Hydrolysis of Mackerel (Rastrelliger sp.) Muscle Protein Hydrolysate Using Response Surface Methodology
Authors: Riyadi P H , Romadhon  , Bramantyo L. , Arifin M H 
Published: August 2023

Abstract
Mackerel (Rastrelliger sp.) is a widely distributed epipelagic species in South East Asia. Mackerel has a high amount nutrient such as protein (20.83 %) and fat (1.03 %). The high amount of protein and low amount of fat will allow it to be used as a material to produce a good protein hydrolysate. The aim of this study is to determine the optimal enzymatic hydrolysis conditions (time, temperature, and pH) using Response Surface Methodology (RSM). Mackerel Protein Hydrolysate (MPH) was prepared using commercial Flavourzyme. Optimization of MPH was performed by employing Box Behnken Design method of RSM. SN-TCA method was used to calculate the degree of hydrolysis (DH) which is the key parameter in hydrolysis reaction. Optimum hydrolysis conditions were obtained at pH 7, temperature 55°C and 60 min of process. Under these conditions the DH obtained was 17.7293 % with 4% enzyme to substrate ratio. The suggested model for the hydrolysis process is quadratic with the desirability factor of 1. The MPH was further assessed for its amino acid composition using High Performance Liquid Chromatography (HPLC). The hydrolysis process increases the amino acid amounts namely L-Glutamic Acid (19.77%), L-Valin (14.20%), L-Aspartic Acid (11.42%), Glycine (11.04%), L-Alanin (14.20%), L-Prolin (16.80%), and L-Histidin (27.06%). The study suggested that mackerel muscle can be considered to be utilized as fish protein hydrolysis materials.