bluuebunny/crossref_metadata_2025_split huggingface dataset:
The endophytobiome of wild Rubiaceae as a source of antagonistic fungi against the American Leaf Spot of coffee (Mycena citricolor)
Authors: Escudero-Leyva Efraín , Granados-Montero María del Milagro , Orozco-Ortiz Cristofer , Araya-Valverde Emmanuel , Alvarado-Picado Eduardo , Chaves-Fallas José Miguel , Aldrich-Wolfe Laura , Chaverri Priscila 
Published: April 2023

Abstract

Aims
The American leaf spot, caused by Mycena citricolor, is an important disease of coffee (Coffea arabica), mostly in Central America. Currently, there are limited pathogen control alternatives that are environment friendly and economically accessible. The use of fungi isolated from the plant endomycobiota in their native habitats is on the rise because studies show their great potential for biological control. To begin to generate a green alternative to control M. citricolor, the objectives of the present study were to (i) collect, identify, screen (in vitro and in planta), and select endophytic fungi from wild Rubiaceae collected in old-growth forests of Costa Rica; (ii) confirm endophytic colonization in coffee plantlets; (iii) evaluate the effects of the endophytes on plantlet development; and (iv) corroborate the antagonistic ability in planta.

Methods and results
Through in vitro and in planta antagonism assays, we found that out of the selected isolates (i.e. Daldinia eschscholzii GU11N, Nectria pseudotrichia GUHN1, Purpureocillium aff. lilacinum CT24, Sarocladium aff. kiliense CT25, Trichoderma rifaii CT5, T. aff. crassum G1C, T. aff. atroviride G7T, T. aff. strigosellum GU12, and Xylaria multiplex GU14T), Trichoderma spp. produced the highest growth inhibition percentages in vitro. Trichoderma isolates CT5 and G1C were then tested in planta using Coffea arabica cv. caturra plantlets. Endophytic colonization was verified, followed by in planta growth promotion and antagonism assays.

Conclusions
Results show that Trichoderma isolates CT5 and G1C have potential for plant growth promotion and antagonism against Mycena citricolor, reducing incidence and severity, and preventing plant mortality.
In Memoriam: Fred M. Utter, a founder of fisheries genetics
Authors: Allendorf Fred W , Ryman Nils , Waples Robin S 
Published: April 2023

Abstract
Fred Utter died in his sleep during the night of 5 March 2023 at the age of 91. In this paper, 3 friends and colleagues of Fred describe the important role that his genetics research has played in the management and conservation of fish populations.
Visibility or Impact? International Efforts to Defend LGBTQI+ Rights in Africa
Authors: Brown Stephen 
Published: April 2023

Abstract
Most struggles for LGBTQI+ rights play out at the national level. However, the question of sexual and gender minorities’ rights periodically appears as a point of friction in international relations as well. This article analyses the question of international efforts to defend LGBTQI+ rights in countries of the Global South, with a particular focus on Western countries’ endeavours in Africa. Combining policy analysis, critique and recommendations, it asks how and when international actors should and should not intervene. It recognizes that motives for intervening can be problematic and the means often counterproductive, especially when exhibiting neo-imperialist tendencies and constituting ad hoc reactions to events in the media. Countering essentialist arguments about ‘authentic’ African culture and values, influenced by religious beliefs, is also a significant challenge. I argue that more fruitful efforts should instead be centred on local rights defenders’ perspectives and supporting their priorities and initiatives, based on concerted, long-term, principled strategies. International actors, however, are reluctant to adopt such approaches because of a desire for short-term visible action, even if less effective or not effective at all. This conundrum is a fundamental problem in the area of foreign aid writ large, as greater impact often requires less visibility on the part of international actors, but donor countries want domestic and international recognition of their efforts. The article distils key lessons learnt and principles for action that have emerged over the past 15 to 20 years, brought together in one place for the first time. It aims to stimulate discussions among practitioners and academics. It should be of particular interest to human rights practitioners, especially those who are involved or contemplating getting involved in defending the rights of sexual and gender minorities internationally.
Emergency contraception – pharmacists’ knowledge and attitude on emergency contraceptives’ dispensing practices
Authors: Akande-Sholabi Wuraola , Dehinde-Joseph Valerie , Showande Segun Johnson 
Published: April 2023

Abstract

Objectives
Emergency contraceptives have the potential to reduce health complications in women with unintended/unwanted pregnancies. Access to availability and use of emergency contraceptive pills (ECPs) is influenced by pharmacist knowledge and attitudes, and there are limited data in Nigeria on pharmacists’ ECPs dispensing practices. This study assessed pharmacists’ knowledge, attitude and practice towards the use of emergency contraceptives.

Methods
A cross-sectional study conducted among 100 community pharmacists in Ibadan metropolis, Nigeria with the use of a self-administered structured questionnaire. The questionnaire contained Likert-type 17-item knowledge, 9-item attitude and 15-item practice scales. The scales utilised 5–7 graded responses. The scaled score was graded as good and poor knowledge, positive and negative attitude and high, moderate or low-level practice of emergency contraception. The ability to predict the level of practice of emergency contraception, based on the pharmacist’s knowledge and attitude towards the use of ECP, was determined with hierarchical multiple regression as P &lt; 0.05.

Key findings
Good knowledge of emergency contraceptives was displayed by 51.5% of the pharmacists, and 51.9% had a positive attitude towards the use of ECPs. The level of ECP dispensing practices was high among 43.4% of pharmacists and low among 30.1% of pharmacists. More than 30% of the pharmacists offer a high level of educational services practice for ECPs. Pharmacists’ knowledge (β = 0.348, P = 0.002) and attitude (β = −0.302, P = 0.007) were predictive of the dispensing practice of ECPs.

Conclusion
The community pharmacists have good knowledge of emergency contraceptive use and showed a positive attitude towards the dispensing of emergency contraceptive pills.
Adverse childhood experiences and sexual dysfunction in midlife women: Is there a link?
Authors: Saadedine Mariam , Faubion Stephanie , Kingsberg Sheryl , Enders Felicity , Kuhle Carol , Kling Juliana M , Mara Kristin , Kapoor Ekta 
Published: April 2023

Abstract

Background
One in 3 children has had at least 1 adverse childhood experience (ACE), and ACEs have been associated with multiple medical and psychiatric morbidities in women later in life, including greater menopause symptom burden.

Aim
To evaluate the association between ACEs and female sexual dysfunction (FSD) in midlife women.

Methods
A cross-sectional analysis from DREAMS—the Data Registry on Experiences of Aging, Menopause, and Sexuality—was conducted with questionnaires completed by women aged 40 to 65 years who presented to a women’s health clinic at Mayo Clinic in Rochester, Minnesota, from May 2015 to December 2016. History of ACEs was obtained with the validated ACE questionnaire. FSD was assessed by the Female Sexual Function Index and the Female Sexual Distress Scale–Revised.

Outcomes
The association between ACEs and FSD (defined as Female Sexual Function Index score ≤26.55 and Female Sexual Distress Scale–Revised score ≥11) was evaluated via a multivariable logistic regression model, adjusting for age, menopause status, hormone therapy use, anxiety, depression, relationship satisfaction, hot flash severity, and history of abuse in the past year.

Results
Women (N = 1572) had a mean age of 53.2 years. Overall 59% reported having at least 1 ACE. When compared with no ACEs, a history of ≥4 ACEs significantly increased the odds of not being sexually active (odds ratio, 1.83; 95% CI, 1.30-2.57; P &lt; .001). Among sexually active women, the proportion of women with FSD increased sequentially as the number of ACEs increased. In the univariate analysis, a history of ≥4 ACEs significantly increased the odds of FSD as compared with no ACEs (odds ratio, 2.12; 95% CI, 1.50-2.99; P &lt; .001). The association remained statistically significant in the multivariable analysis after adjusting for confounders (odds ratio, 1.75; 95% CI, 1.15-2.68; P = .009).

Clinical Implications
The findings highlight an opportunity for clinicians to screen for ACEs in women with sexual dysfunction and offer appropriate treatment and counseling as indicated.

Strengths and Limitations
Strengths of the study include the large cohort, the use of validated tools for assessment of ACEs and FSD, and the adjustment for multiple potential confounding factors. Limitations include the cross-sectional study design, recall bias in reporting ACEs and recent abuse, and the low representation of racially and ethnically diverse women in the cohort.

Conclusion
The study demonstrates an increased risk of sexual inactivity and sexual dysfunction in midlife women who experienced childhood adversity. The sexual dysfunction in women with ACEs seems to be independent of other factors that potentially affect female sexual function in midlife.
Plant species with larger extrafloral nectaries produce better quality nectar when needed and interact with the best ant partners
Authors: Alencar Cícero Luanderson da Silva , Nogueira Anselmo , Vicente Ricardo Eduardo , Coutinho Ítalo Antônio Cotta 
Published: April 2023

Abstract
Few studies have explored the phenotypic plasticity of nectar production on plant attractiveness to ants. Here, we investigate the role of extrafloral nectary (EFN) size on the productivity of extrafloral nectar in three sympatric legume species. We hypothesized that plant species with larger EFNs (i) have higher induced nectar secretion after herbivory events, and (ii) are more likely to interact with more protective (i.e. dominant) ant partners. We target 90 plants of three Chamaecrista species in the field. We estimated EFN size and conducted field experiments to evaluate any differences in nectar traits before and after leaf damage to investigate the phenotypic plasticity of nectar production across species. We conducted multiple censuses of ant species feeding on EFNs over time. Plant species increased nectar descriptors after leaf damage, but in different ways. Supporting our hypothesis, C. duckeana, with the largest EFN size, increased all nectar descriptors, with most intense post-herbivory-induced response, taking its place as the most attractive to ants, including dominant species. EFN size variation was an excellent indicator of nectar productivity across species. The higher control over reward production in plants with larger sized EFNs reflects an induction mechanism under damage that reduces costs and increases the potential benefits of indirect biotic defences.
“I would I had that corporal soundness”: Pervez Rizvi's Analysis of the Word Adjacency Network Method of Authorship Attribution
Authors: Egan Gabriel , Eisen Mark , Ribeiro Alejandro , Segarra Santiago 
Published: April 2023

Abstract
In his two-part article ‘An Analysis of the Word Adjacency Network Method—Part 1—The evidence of its unsoundness’ and ‘Part 2—A true understanding of the method’ Digital Scholarship in the Humanities, 38: 347-78 (2022), Pervez Rizvi attempts to replicate the Word Adjacency Network (WAN) method for authorship attribution and show that it does not produce the new knowledge that we, its inventors, claim for it. In the present essay, we will show that Rizvi misrepresents fundamental aspects of the WAN method, that his attempted replication fails not because the method is flawed but because he erred in replicating it, and that Rizvi misunderstands key aspects of the mathematics of Information Theory that the method uses.
Massive core/star formation triggered by cloud–cloud collision – II. High-speed collisions of magnetized clouds
Authors: Sakre Nirmit , Habe Asao , Pettitt Alex R , Okamoto Takashi , Enokiya Rei , Fukui Yasuo , Hosokawa Takashi 
Published: April 2023

ABSTRACT
We present a study of the effects of magnetic fields on the formation of massive, self-gravitationally bound cores (MBCs) in high-speed cloud–cloud collisions (CCCs). Extending our previous work, we perform magnetohydrodynamic simulations of the high-speed (20–40 km s−1) collisions between two magnetized (initial 4 $\mu\!$ G), turbulent clouds of different sizes in the range of 7–20 pc. We show that a magnetic field hinders the growth of cores, particularly in the case of short-duration collisions where cores are not seen to reach a highly bound state. In such cases, a shocked region created by the collision rapidly expands into the ambient medium owing to the enhanced magnetic pressure, resulting in the destruction of the highly unbound cores and suppression of gas accretion. This negative effect on the formation of MBCs has not been reported in previous hydrodynamic simulations of CCCs. Together with our previous work, we conclude that magnetic fields provide two competing effects on the MBC formation in CCCs; while they promote the mass accumulation onto cores during a collision, they operate to destroy cores or hinder the core growth after the collision. The duration of such collisions determines which effect prevails, resulting in a maximum collision speed for the MBC formation within given colliding clouds. Our results agree with the observed trend among CCC samples in the column density range probed in the simulations presented here; CCCs with higher relative velocities require higher column densities for the formation of massive stars.
Galaxy quenching time-scales from a forensic reconstruction of their colour evolution
Authors: Bravo Matías , Robotham Aaron S G , Lagos Claudia del P , Davies Luke J M , Bellstedt Sabine , Thorne Jessica E 
Published: April 2023

ABSTRACT
The time-scales on which galaxies move out of the blue cloud to the red sequence ($\tau ^{}_\mathrm{Q}$ ) provide insight into the mechanisms driving quenching. Here, we build upon previous work, where we showcased a method to reconstruct the colour evolution of observed low-redshift galaxies from the Galaxy And Mass Assembly (GAMA) survey based on spectral energy distribution (SED) fitting with ProSpect, together with a statistically driven definition for the blue and red populations. We also use the predicted colour evolution from the shark semi-analytic model, combined with SED fits of our simulated galaxy sample, to study the accuracy of the measured $\tau ^{}_\mathrm{Q}$ and gain physical insight into the colour evolution of galaxies. In this work, we measure $\tau ^{}_\mathrm{Q}$ in a consistent approach for both observations and simulations. After accounting for selection bias, we find evidence for an increase in $\tau ^{}_\mathrm{Q}$ in GAMA as a function of cosmic time (from $\tau ^{}_\mathrm{Q}$ ∼ 1 Gyr to $\tau ^{}_\mathrm{Q}$ ∼ 2 Gyr in the lapse of ∼4 Gyr), but not in shark ($\tau ^{}_\mathrm{Q}$ ≲ 1 Gyr). Our observations and simulations disagree on the effect of stellar mass, with GAMA showing massive galaxies transitioning faster, but is the opposite in shark. We find that environment only impacts galaxies below ∼1010 M⊙ in GAMA, with satellites having shorter $\tau ^{}_\mathrm{Q}$ than centrals by ∼0.4 Gyr, with shark only in qualitative agreement. Finally, we compare to previous literature, finding consistency with time-scales in the order of couple Gyr, but with several differences that we discuss.
A radio-emitting outflow produced by the tidal disruption event AT2020vwl
Authors: Goodwin A J , Alexander K D , Miller-Jones J C A , Bietenholz M F , van Velzen S , Anderson G E , Berger E , Cendes Y , Chornock R , Coppejans D L , Eftekhari T , Gezari S , Laskar T , Ramirez-Ruiz E , Saxton R 
Published: April 2023

ABSTRACT
A tidal disruption event (TDE) occurs when a star is destroyed by a supermassive black hole. Broad-band radio spectral observations of TDEs trace the emission from any outflows or jets that are ejected from the vicinity of the supermassive black hole. However, radio detections of TDEs are rare, with &lt;20 published to date, and only 11 with multi-epoch broad-band coverage. Here we present the radio detection of the TDE AT2020vwl and our subsequent radio monitoring campaign of the outflow that was produced, spanning 1.5 yr post-optical flare. We tracked the outflow evolution as it expanded between 1016 and 1017 cm from the supermassive black hole, deducing it was non-relativistic and launched quasi-simultaneously with the initial optical detection through modelling the evolving synchrotron spectra of the event. We deduce that the outflow is likely to have been launched by material ejected from stream-stream collisions (more likely), the unbound debris stream, or an accretion-induced wind or jet from the supermassive black hole (less likely). AT2020vwl joins a growing number of TDEs with well-characterized prompt radio emission, with future timely radio observations of TDEs required to fully understand the mechanism that produces this type of radio emission in TDEs.
The effect of stellar encounters on the dark matter annihilation signal from prompt cusps
Authors: Stücker Jens , Ogiya Go , White Simon D M , Angulo Raul E 
Published: April 2023

ABSTRACT
Prompt cusps are the densest quasi-equilibrium dark matter objects; one forms at the instant of collapse within every isolated peak of the initial cosmological density field. They have power-law density profiles, ρ ∝ r−1.5 with central phase-space density set by the primordial velocity dispersion of the dark matter. At late times, they account for $\sim 1~{{\ \rm per\ cent}}$ of the dark matter mass but for $\gt 90~{{\ \rm per\ cent}}$ of its annihilation luminosity in all but the densest regions, where they are tidally disrupted. Here we demonstrate that individual stellar encounters rather than the mean galactic tide are the dominant disruptors of prompt cusps within galaxies. Their cumulative effect is fully (though stochastically) characterized by an impulsive shock strength $B_* = 2\pi G\int \rho _*({\bf x}(t))\, \mathrm{d}t$ where ρ*, the total mass density in stars, is integrated over a cusp’s entire post-formation trajectory. Stellar encounters and mean tides have only a small effect on the halo annihilation luminosity seen by distant observers, but this is not true for the Galactic halo because of the Sun’s position. For a 100 GeV WIMP, Earth-mass prompt cusps are predicted, and stellar encounters suppress their mean annihilation luminosity by a factor of two already at 20 kpc, so that their annihilation emission is predicted to appear almost uniform over the sky. The Galactic centre γ-ray excess is thus unaffected by cusps. If it is indeed dark matter annihilation radiation, then prompt cusps in the outer Galactic halo and beyond must account for 20–80 per cent of the observed isotropic γ-ray background in the 1–10 GeV range.
From methodological limitations to the function of metallothioneins - a guide to approaches for determining weak, moderate, and tight affinity zinc sites
Authors: Pomorski Adam , Drozd Agnieszka , Kocyła Anna , Krężel Artur 
Published: April 2023

Abstract
Mammalian metallothioneins (MTs) are small cysteine-rich proteins whose primary role is participation in zinc and copper homeostasis. Ever since their discovery, MTs have been investigated in terms of metal-binding affinity. The initial concept of seven Zn(II) ions (Zn7MT) bound with the same, undifferentiated low-picomolar affinity in the α and β domains prevailed for many years and derived from spectroscopic studies. The application of fluorescent zinc probes has changed the perception of MTs, showing that they function in nanomolar to subnanomolar free zinc concentrations due to the presence of tight, moderate, and weak binding sites. The discovery of Zn(II)-depleted MTs in many tissues and determination of cellular free Zn(II) concentrations with differentiated zinc affinity sites revealed the critical importance of partially saturated Zn4–6MTs species in cellular zinc buffering in a wide picomolar to nanomolar range of free Zn(II) concentrations. Until today, there was no clear agreement on the presence of differentiated or only tight zinc sites. Here, we present a series of spectroscopic, mass spectrometry-based, and enzymatic competition experiments that reveal how weak, moderate, or high-affinity ligands interact with human MT2, with special attention to the determination of Zn(II) affinities. The results show that the simplification of the stability model is the major reason for determining significantly different stability data that obscured the actual MTs function. Therefore, we emphasize that different metal affinities are the single most important reason for their presumed function, which changed over the years from tight binding and, thus, storage to one that is highly dynamic.
Led-Seq: ligation-enhanced double-end sequence-based structure analysis of RNA
Authors: Kolberg Tim , von Löhneysen Sarah , Ozerova Iuliia , Wellner Karolin , Hartmann Roland K , Stadler Peter F , Mörl Mario 
Published: April 2023

AbstractStructural analysis of RNA is an important and versatile tool to investigate the function of this type of molecules in the cell as well as in vitro. Several robust and reliable procedures are available, relying on chemical modification inducing RT stops or nucleotide misincorporations during reverse transcription. Others are based on cleavage reactions and RT stop signals. However, these methods address only one side of the RT stop or misincorporation position. Here, we describe Led-Seq, a new approach based on lead-induced cleavage of unpaired RNA positions, where both resulting cleavage products are investigated. The RNA fragments carrying 2′, 3′-cyclic phosphate or 5′-OH ends are selectively ligated to oligonucleotide adapters by specific RNA ligases. In a deep sequencing analysis, the cleavage sites are identified as ligation positions, avoiding possible false positive signals based on premature RT stops. With a benchmark set of transcripts in Escherichia coli, we show that Led-Seq is an improved and reliable approach based on metal ion-induced phosphodiester hydrolysis to investigate RNA structures in vivo.