bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contribution of pecan (Carya illinoinensis [Wangenh.| K. Koch) to Sustainable Development Goal 2 under the dual perspective of carbon storage and human nutrition
Authors: Cambareri Gustavo , Frusso Enrique Alberto , Herrera-Aguirre Esteban , Zoppolo Roberto , Leite Fernanda Figueiredo Granja Dorilêo , Beltrán Marcelo , Martins Carlos , Mendoza Carlos 
Published: April 2023

This work aims to contextualize and analyze the potential contribution of pecan to SDG2 under the dual perspective of carbon storage and human nutrition. Particularly, the study focuses on the pecan agroecosystems in the Americas, representing the most important pecan-producing countries (the United States, Mexico, Brazil, Argentina, Uruguay, and Peru). We observed that pecan is a reliable sink for storing atmospheric C and also for quality nuts with high nutritional density. The Americas, hold a population of ca. 23 M pecan trees, with the younger tree populations and the highest C-storing potential in South America. This pecan tree population has removed 51.3 Mt CO2eq immobilizing the OC in their aboveground biomass, but if the C sequestration for the whole system is considered, the value reaches nearly 80 Mt CO2eq. From a nutritional perspective, there are different dietary needs to cover according to the country, although the common analysis output is a low proportion of nuts in the diet, which is expected to improve, given the efforts of each country to promote domestic consumption. All the mentioned countries in this study have a low pecan consumption going from 8 to 293 g per capita yr-1, which in the light of the Global Burden of Disease represents 0.08 to 3.2% of the recommended yearly dietary basis for nuts overall. The inclusion of pecan nuts in the daily diet is of utmost importance to offset the food nutrient dilution carbohydrates-based, linked to the excess of atmospheric CO2. Also, pecan orchards function as a platform to integrate sustainable systems. The global benefit of having pecan and alley crops has been proved in regions other than the Americas with interesting economic outputs leading to energizing the life of rural communities. Pecan orchards and pecan agroforestry may lead to sustainable agri-food systems, with global gains in SOC and nutritional richness and diversity. Therefore, more in-depth studies are needed not only to fully understand the functioning of the systems at a productive level but also to design and plan sustainable landscapes in rural land.
Flow-controlled expiration reduces positive end-expiratory pressure requirement in dorsally recumbent, anesthetized horses
Authors: Brandly Jerrianne E. , Midon Monica , Douglas Hope F. , Hopster Klaus 
Published: April 2023

IntroductionEquine peri-anesthetic mortality is higher than that for other commonly anesthetized veterinary species. Unique equine pulmonary pathophysiologic aspects are believed to contribute to this mortality due to impairment of gas exchange and subsequent hypoxemia. No consistently reliable solution for the treatment of peri-anesthetic gas exchange impairment is available. Flow-controlled expiration (FLEX) is a ventilatory mode that linearizes gas flow throughout the expiratory phase, reducing the rate of lung emptying and alveolar collapse. FLEX has been shown to improve gas exchange and pulmonary mechanics in anesthetized horses. This study further evaluated FLEX ventilation in anesthetized horses positioned in dorsal recumbency, hypothesizing that after alveolar recruitment, horses ventilated using FLEX would require a lower positive end-expiratory pressure (PEEP) to prevent alveolar closure than horses conventionally ventilated.MethodsTwelve adult horses were used in this prospective, randomized study. Horses were assigned either to conventional volume-controlled ventilation (VCV) or to FLEX. Following induction of general anesthesia, horses were placed in dorsal recumbency mechanically ventilated for a total of approximately 6.5 hours. Thirty-minutes after starting ventilation with VCV or FLEX, a PEEP-titration alveolar recruitment maneuver was performed at the end of which the PEEP was reduced in decrements of 3 cmH2O until the alveolar closure pressure was determined. The PEEP was then increased to the previous level and maintained for additional three hours. During this time, the mean arterial blood pressure, pulmonary arterial pressure, central venous blood pressure, cardiac output (CO), dynamic respiratory system compliance and arterial blood gas values were measured.ResultsThe alveolar closure pressure was significantly lower (6.5 ± 1.2 vs 11.0 ± 1.5 cmH2O) and significantly less PEEP was required to prevent alveolar closure (9.5 ± 1.2 vs 14.0 ± 1.5 cmH2O) for horses ventilated using FLEX compared with VCV. The CO was significantly higher in the horses ventilated with FLEX (37.5 ± 4 vs 30 ± 6 l/min).DiscussionWe concluded that FLEX ventilation was associated with a lower PEEP requirement due to a more homogenous distribution of ventilation in the lungs during expiration. This lower PEEP requirement led to more stable and improved cardiovascular conditions in horses ventilated with FLEX.
LcWRKY17, a WRKY Transcription Factor from Litsea cubeba, Effectively Promotes Monoterpene Synthesis
Authors: Gao Jing , Chen Yicun , Gao Ming , Wu Liwen , Zhao Yunxiao , Wang Yangdong 
Published: April 2023

The WRKY gene family is one of the most significant transcription factor (TF) families in higher plants and participates in many secondary metabolic processes in plants. Litsea cubeba (Lour.) Person is an important woody oil plant that is high in terpenoids. However, no studies have been conducted to investigate the WRKY TFs that regulate the synthesis of terpene in L. cubeba. This paper provides a comprehensive genomic analysis of the LcWRKYs. In the L. cubeba genome, 64 LcWRKY genes were discovered. According to a comparative phylogenetic study with Arabidopsis thaliana, these L. cubeba WRKYs were divided into three groups. Some LcWRKY genes may have arisen from gene duplication, but the majority of LcWRKY evolution has been driven by segmental duplication events. Based on transcriptome data, a consistent expression pattern of LcWRKY17 and terpene synthase LcTPS42 was found at different stages of L. cubeba fruit development. Furthermore, the function of LcWRKY17 was verified by subcellular localization and transient overexpression, and overexpression of LcWRKY17 promotes monoterpene synthesis. Meanwhile, dual-Luciferase and yeast one-hybrid (Y1H) experiments showed that the LcWRKY17 transcription factor binds to W-box motifs of LcTPS42 and enhances its transcription. In conclusion, this research provided a fundamental framework for future functional analysis of the WRKY gene families, as well as breeding improvement and the regulation of secondary metabolism in L. cubeba.
Influence of the Antimicrobial LL-37 Peptide on Legionella dumoffii Phospholipids Adsorbed at the Air–Liquid Interface
Authors: Pastuszak Katarzyna , Kowalczyk Bożena , Tarasiuk Jacek , Jurak Małgorzata , Palusińska-Szysz Marta 
Published: April 2023

Legionella dumoffii is an intracellular pathogen of freshwater protozoans capable of infecting and multiplying in mammalian cells, causing a severe respiratory disease called Legionnaires’ disease. The pathomechanism of infection development is very complex and depends on many factors, including the structure and properties of macromolecules that build the components of the L. dumoffii cell envelope. Phospholipids (PLs) forming biological membranes have a significant impact on the integrity of the membrane as well as on the interactions with the host cells. L. dumoffii changes its lipid profile under the influence of external factors, which allows it to adapt to the living environment. One of the factors altering the PL composition is the presence of exogenous choline. The aim of this study was to determine the physicochemical properties of the model bacterial membranes adsorbed at the air–liquid interface (Langmuir monolayers). They were composed of phospholipids isolated from L. dumoffii cultured with (PL+choline) and without (PL−choline) choline. Moreover, the effect of the human cathelicidin (LL-37 peptide) added to the subphase on these monolayers was analyzed in terms of phospholipid–peptide interactions. The results indicated that the monolayers of PL+choline were slightly more condensed than PL−choline. In the presence of LL-37, the elasticity of both monolayers increased; thus, their molecular packing and ordering decreased. The disturbing effect was related to the peptide’s antibacterial activity.
Virome of ancient wolf mummy (<i>Canis lupus</i>) as a potential source of virulent bacteriophages for phage therapy
Authors: Goncharov A. E. , Ahremenko Ya. A. , Azarov D. V. , Cheprasov M. J. , Fedorov S. E. , Solomenny A. P. , Kolodzhieva V. V. 
Published: April 2023

Aim. To investigate the potential for searching new virulent bacteriophages in the paleontological material extracted from the permafrost zone.Material and methods. The virome structure of the colon content of the wolf pup mummy (Canis lupus) from the late Pleistocene was evaluated by means of shotgun metagenomic sequencing.Results. The study demonstrated the predominance of Myoviridae tailed bacteriophages, including PhiKZ-like phages, in the structure of the virome.Conclusion. The results of the study indicate the possibility of using paleontological material preserved in the ancient Arctic permafrost as a resource for searching and isolating new virulent bacteriophages.
Association of gene polymorphisms of angiotensin metabolism with left ventricular diastolic dysfunction in hypertensive patients
Authors: Kalinkina T. V. , Lareva N. V. , Chistyakova M. V. , Serkin M. A. 
Published: April 2023

Aim. To investigate the relationship of SNP ACE ((AluIns/DelI>D), rs 4646994), SNP of angiotensinogen 1 gene AGT :521 C>T ((Thr 174 Met), rs 4762), SNP of angiotensinogen 2 gene AGT:704 T>C ((Met 235 Thr), rs699), SNP of angiotensin-2 type 1 receptor gene AGTR1: A1166C; A>C (rs5186) with left ventricular diastolic dysfunction in hypertensive patients.Methods and materials. 74 patients (26 women and 48 men) with stage 1-2 hypertension were examined. The analysis of genetic polymorphisms was carried out by means of polymerase chain reaction method with the analysis of DNA RFLP (restriction fragment length polymorphism).Results. CC polymorphisms of genotype AGT:704 T>C (Met 235 Thr), (χ2 = 8.18; p = 0.017) were detected in the group of patients with left ventricular diastolic dysfunction, which nearly triples the probability of impaired myocardial relaxation (OR 2.85) and CC polymorphisms of genotype of angiotensinogen type 1 receptor gene (AGTR1: A1166C), (χ2 = 1.77; p = 0.041), which doubles the probability of left ventricular diastolic dysfunction (OR 2.39).Conclusion. CC genotype AGT:704 T>C (Met 235 Thr) and CC genotype of the angiotensinogen type 1 receptor gene (AGTR1: A1166C) are associated with the development of left ventricular diastolic dysfunction in patients with hypertension.
<i>Paragonimus westermani ichunensis:</i> evolution of parasitological situation
Authors: Popov A. F. , Besprozvannykh V. V. , Sukhanova G. I. , Ivanis V. A. , Kolpakov S. L. 
Published: April 2023

The paper presents an analysis of parasitological situation with paragonimiasis in Primorsky Krai over the past 30 years. In the early 1990s regular medical checkups detected paragonimiasis in 1–6% of the inhabitants of the region. Since 1996, the situation in the south of the Russian Far East has changed dramatically. Within 1–2 years, the crayfish population decreased significantly in watercourses from southern Primorye up to the Amur basin. Therefore, the incidence of the disease in population of the region has decreased to zero level since 2000. From 2014 the number of crayfish in watercourses of Primorsky Krai started growing. In 2019 the first patient with larval paragonimiasis was reported. The paper reviews the experience of the Regional Clinical Hospital of Vladivostok in treatment of 368 patients with larval paragonimiasis for the period from 1967 to 1996. On average, the disease manifested itself in 1–4 weeks from the moment of infection. The diagnosis was confirmed in 97.9% of cases via serological method. The praziquantel treatment of patients with larval paragonimiasis resulted in a cure rate of 89%. The disease duration ranged from 1–2 to 10 or more years.
Bacterial biofilm formation on ceramics of various composition used for orthopedic endoprosthetics
Authors: Skurikhina Yu. E. , Papynov E. K. , Zaitseva E. A. , Shichalin O. O. 
Published: April 2023

Aim. To assess the intensity and characteristics of bacterial biofilms formed by non-fermenting gram-negative bacteria Pseudomonas aeruginosa and Acinetobacter baumannii on ceramic biocomposite samples based on wollastonite and zirconia, including those modified with the bioactive phase of hydroxyapatite (HA).Materials and methods. Biofilms formed on bioceramic samples, prepared according to the author’s original method, were examined by scanning electron microscopy (SEM). The studied samples included non-composite ceramics based on wollastonite (CaSiO3); composite ceramics based on wollastonite with HA (CaSiO3-HA); non-composite ceramics based on zirconium dioxide ZrO2; ceramics based on zirconia with HA 15 wt% (ZrO2-(15 wt % HA); ceramics based on zirconia with (ZrO2-(50 wt% HA). Biofilms were obtained by placing the samples in a nutrient medium with P. aeruginosa and A. baumannii. Cultivation was carried out at 37 °С for 24 and 48 h, respectively. The dynamics of bacterial growth of the surface of the studied samples was assessed by analyzing SEM images using an LBP classifier.Results. A noticeable difference was observed in the activity and structure of bacterial biofilm formation on all studied samples. The intensity of biofilm formation (surface coverage of ZrO2-based samples) determined using an LBP-based classifier was as follows: ZrO2 ceramics without HA – 28.13%; ZrO2 + 15 wt% HA – 28.33%; and ZrO2 + 50 wt% HA – 88.46%. All samples with HA addition demonstrated higher susceptibility to biofilm formation.Conclusion. The presence of HA in the composition of bioceramics increases the intensity of biofilm formation. This also indicates a higher biocompatibility of such materials. When selecting bioceramic materials for bone defect repair, preference should be given to materials containing no more than 15 wt% HA in the view of reducing the risk of infection.
Accelerated bacteriological examination of urine and dialysate in the diagnosis of urinary tract infections in children
Authors: Boronina L. G. , Samatova E. V. , Ustyugova S. S. , Panova S. A. , Kutsevalova O. Y. 
Published: April 2023

Aim. To evaluate the feasibility of an accelerated method for determining the etiology of infections in urine, dialysate and residual antimicrobial activity by using laser light scattering technology.Materials and methods. From January to September 2019, 106 urine samples and 42 dialysate samples from children aged 1–16 with various urinary pathologies and those on peritoneal dialysis underwent culture-based examination on an ALIFAX HB&L LIGHT analyzer (Alifax, Italy) using laser light scattering technology.Results. Three hours after inoculation, 81 samples (76.4%) appeared to be negative and 25 samples (23.6%) proved to be positive. No microorganisms were detected in 38 dialysate samples (90.5%), two samples had Staphylococcus aureus (104 CFU/ml), and one dialysate sample had Corynebacterium sp. and associations of Escherichia coli + Candida albicans + Staphylococcus haemolyticus, associated with peritoneal catheter colonization. Bacteriuria was caused by Enterobacterales in 39.3% (E. coli, Morganella morganii, Citrobacter amalonaticus, Enterobacter cloacae), non-fermenting gram-negative bacteria – in 14.3% (Pseudomonas aeruginosa, Ralstonia picketii, Oligella sp., Acinetobacter baumannii), Enterococcus sp. – 21.4%, S. haemolyticus, Staphylococcus epidermidis – 10.7%, Candida albicans – 3.6%, contamination: Streptococcus viridians, Corynebacterium sp. – 7.1%. Residual antimicrobial activity in urine was detected in 30.1% of patients.Conclusion. The laser light scattering technology enables a minimal concentration of microorganisms to be detected in a smaller amount of urine or dialysate, which is very important for accelerated diagnosis of urinary tract infections and complications of peritoneal dialysis in children.
Anti-bacterial and anti-biofilm activity of bacteriophages against <i>Klebsiella pneumoniae</i> and <i>Pseudomonas aeruginosa</i> isolated from orthopedic patients
Authors: Gordina E. M. , Bozhkova S. A. , Smirnova L. N. 
Published: April 2023

Objective. To investigate the susceptibility of K. pneumoniae and P. aeruginosa to a polyvalent bacteriophage preparation and its effect on biofilm formation and the strain biofilms isolated from orthopedic patients.Materials and methods. The research sample included 50 clinical isolates of K. pneumoniae and 50 clinical isolates of P. aeruginosa. Identification was performed by MALDI-TOF-MS; antibiotic susceptibility was assessed in accordance with EUCAST v 21. Detection of carbapenemase genes was carried out by real-time PCR. The strain susceptibility to the bacteriophage was determined by a spot test; K. pneumoniae ATCC 33495 and P. aeruginosa ATCC 27853 were determined by assessing their growth curves. Biofilms of strains sensitive to bacteriophages were formed according to the O’Toole method by co-incubation of bacteria with phages. The effect of bacteriophages on 24-hour biofilms was assessed by comparing the optical density of dye extracts of bacteriophage-treated wells and control wells at 570 nm. The data were analyzed using the Statistica environment.Results. It was found that 7 (14%) of K. pneumoniae and 15 (30%) of P. aeruginosa were resistant to carbapenems. Six strains of K. pneumoniae produced NDM-cabapenemase, while four isolates of P. aeruginosa produced VIM-carbapenemases. The bacteriophage preparation under study was active against 36% and 56% of K. pneumoniae and P. aeruginosa strains, respectively. The majority of the studied strains reduced biofilm production upon co-incubation with a phage; however, a decrease in biomass of greater than 80% was observed only for P. aeruginosa. The effect of the bacteriophage on the already formed biofilms was less pronounced, despite a decrease in the biofilm biomass in 78% and 68% of K.pneumoniae and P. aeruginosa strains, respectively.Conclusion. The results obtained confirm the need for further research into the action of bacteriophages against pathogens caused by implant-associated infections and the development of bacteriophage therapy for orthopedic patients.
Simultaneous vegetation of opportunistic oral microorganisms in chronic periodontitis with recurrent course
Authors: Strelnikova N. V. , Tsarev V. N. , Antonova A. A. , Shapovalenko E. S. , Skorobogatova V. E. 
Published: April 2023

Aim. To evaluate clinical significance of simultaneous vegetation by conducting microbiological research of the species composition in the periodontal pockets and on the surface of the tongue root and back in chronic (generalized) periodontitis.Materials and methods. A prospective microbiological analysis of clinically significant species of opportunistic microorganisms in pathological periodontal pockets and the microbiome of the tongue root and back, in chronic (generalized) periodontitis of mild, moderate and severe degree, was carried out in 88 patients of the WHO key group, aged 35–44 (CI 95% 34.8–44.3), of the Unistom Dental Clinic of the Far Eastern State Medical University, Khabarovsk Krai, for the period 2016–2019.Results. The species composition of opportunistic microorganisms in the biotopes of the pathological periodontal pockets and tongue root and back was the same in chronic periodontitis, with a mean value of 89.7% ±2.9% (CI 95% 85.8–94.4). The mean value of the number of species of clinically significant opportunistic microorganisms in the periodontal pockets and root and back of the tongue in associations accounted for 3.9 (CI 95% 1.8–5.4). Bacteria of the genus Leptotrichia were detected simultaneously in periodontal pockets and on the root and back of the tongue in clinically significant associations with Staphylococcus aureus (CI 95% 37.3–65.4), mean Shannon index H value 3.1 ± 0.4; mean Simpson index C value 0.39 ± 0. Bacteria of the genus Leptotrichia in association with Streptococcus pyogenes (CI 95% 35.8–79.3), mean Shannon index H 2.9 ± 0.3; mean value of the Simpson index C 0.28 ± 0.06, reliably support the recurrent course of the opportunistic infectious and inflammatory process in the periodontium. The dominant role of Staphylococcus aureus and Streptococcus pyogenes in the biotopes of periodontal pockets and the root and back of the tongue in exacerbations of chronic periodontitis has been established. Titre of opportunistic microorganisms in pathological periodontal pockets of 2–5lgCFU (colony-forming units) in mild and moderate degree of severity is 1–2 order lower than titre of opportunistic microorganisms in root and back of the tongue of 4–6lgCFU (95% CI 2.1–6.5).Conclusions. Evaluation of simultaneous vegetation of opportunistic microorganisms in the oral microbiome provides a means for improving laboratory (microbiological) diagnostics in chronic periodontitis and applying a non-invasive microbiological method for indirect diagnosis of the species composition of opportunistic microorganisms in periodontal pockets according to the composition of opportunistic microorganisms of the root and back of the tongue in chronic periodontitis for prophylaxis and treatment personalization.
Bioprofiles of <i>Staphylococcus spp.</i> isolated from prostate secretion in men with chronic bacterial prostatitis
Authors: Kartashova O. L. , Pashinina O. A. , Pashkova T. M. , Gritsenko V. A. , Mikhailenko S. V. 
Published: April 2023

Objective. To characterize pathogenic pheno- and genoprofiles of staphylococci of different species, isolated from the secretion of the prostate gland in male patients with chronic bacterial prostatitis.Materials and methods. The bacterial spectrum of microflora was studied by a bacteriological method; the species identification of microorganisms was carried out by mass spectrometry. Detection of genes determining pathogenicity factors was carried out by PCR. The biofilm-forming ability of staphylococci, as well as their anti-lysozyme, hemolytic, and adhesive activity, were determined by photometry.Results. Staphylococci were found to be dominant in the pathogen structure of chronic bacterial prostatitis. These microorganisms, regardless of their species, had a pronounced pathogenic potential. Specific features in the bioprofiles of cultures of different species were determined according to the severity of the studied biological properties. Thus, the hemolytic activity and biofilm-forming ability was significantly higher in S. aureus. Conversely, CNS strains were characterized by high anti-lysozyme activity. A significant difference was noted in the prevalence of genes that determine pathogenicity factors in the studied staphylococci of different species.Conclusion. The pathogenic bioprofile of staphylococci of various species isolated from patients with chronic bacterial prostatitis can be used as a criterion in the search and identification of the pathogen, as well as in the development of effective therapeutical approaches.