bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of soft computing in methodology for calculating information protection from parameters of its distribution in social networks
Authors: Sapra Pooja , Paikaray Divya , Gusain Nutan , Abrol Monika , S Ramesh , BHARDWAJ SHAMBHU 
Published: March 2023

Abstract
Security of data has always been a big problem in information technology. Because the data are stored in a variety of locations, including all over the world, this problem becomes even more pressing in the context of cloud computing. Concerns about cloud technology stem primarily from users' concerns regarding data security and privacy. The heterogeneity of cloud resources and the numerous shared applications they serve can benefit from effective scheduling. Considering the quality of the service that is provided to users, this will cut costs and energy use for them. Goal of this study is to improve cloud soft computing's resource allocation and data protection using a secure channel model and machine learning architecture combined with distributed social networks. The cloud architecture data protection in the proposed network model is accomplished by developing the channel model using hierarchical lightweight cryptography analysis. Then, Q-bayes propagation quantum networks are used to allocate resources. Memory capacity, data protection analysis, throughput, end-end delay, and processing time are all used in experimental analysis.Proposed technique attained memory capacity of 73%, data protection analysis of 69%, throughput of 95%, end-end delay of 69%, processing time of 49%.
N-acetyltransferase 10 Promotes Cutaneous Wound Repair via the NF-κB-IL-6 Axis
Authors: Wang Ben , Zhang Jin , Li Guo , Xu Chengzhong , Yang Langmei , Zhang Jie , Liu Zuojun , Wang Ming , Li Ji , Tang Xiaolong , Liu Baohua 
Published: March 2023

Abstract
Cutaneous wound healing, an integral part for protection of skin barrier, is a complex biological process and intimately associated with keratinocyte migration. However, mechanisms regulating keratinocyte migration in the process of cutaneous wound repair remain largely unknown. Here, we found that N-acetyltransferase 10 (NAT10) is essential for cutaneous wound repair in an in vivo skin wound healing model – a significant delay of wound repair in Nat10 haploinsufficient mice and a remarkable inhibition of keratinocyte migration by NAT10 knockdown in an in vitro keratinocyte migration model. We further demonstrate that loss of NAT10 expression attenuates the wound-induced IL-6/IL-8 expression through inhibiting NF-κB/p65 activity in keratinocytes. By deeply digging, silencing NAT10 compromises the level of nuclear p65 by facilitating its poly-ubiquitination, thus accelerates its degradation in the nucleus. Notably, we detected a strong positive correlation between the expression of NAT10 and relevant NF-kB/p65-IL6 signaling activity in mouse wound skin tissues. Overall, our study reveals an important role of NAT10 on cutaneous wound repair by potentiating NF-κB/p65-IL-6/8-STAT3 signaling. Targeting NAT10 might be a potential strategy for the treatment of skin wound dysfunctions and related diseases.
Inflorescence patterns within the genus Portulaca (Portulacaceae): analyses of anatomy and morphology, based on species occurring in Brazil
Authors: Santos Thaíla Vieira Alves dos , Leite Kelly Regina Batista , Trindade Ana Fernanda Guimarães , Mortara Marcos , Hernandes-Lopes José , Ocampo Gilberto , Melo-de-Pinna Gladys Flávia de Albuquerque , de Oliveira Reyjane Patricia 
Published: March 2023

Abstract
The inflorescences of Portulacaceae have traditionally been considered capitula and their morphology and architecture have not been studied in detail. The purpose of this study was to expand the knowledge on the inflorescences within this family, comparing members of distinct lineages of Portulaca occurring in Brazil, to understand some systematic and evolutionary patterns within this group. Morphological characteristics of the inflorescences were analyzed using a light stereomicroscope, and for anatomical studies, we made transversal and longitudinal sections along the entire inflorescence, including involucral bracts, analyzing them using an optical microscope. We confirmed that observed inflorescences are determinate, composed of a first-order dichasium, with second-order helicoidal branches forming a cephalioid structure. The involucral leaves differed from adjacent bracts, which did not display a constriction at the base, as commonly observed in leaves. Thus, we conclude that the congested inflorescence of Portulaca is not a capitulum, but a cephalioid with different degrees of branch reduction within the genus. The Pilosa clade displayed the most congested cephalioid compared to members of the other analyzed clades. We showed present to show a new interpretation of the congested inflorescences in Portulacaand reinforce the need to analyze solitary inflorescences to understand the importance of these data to systematics, evolution, and pollinator-plant interactions within Portulacaceae.
Serological, Morphological and Molecular Diagnosis of Mycobacterium bovis in Cattle at Sulaimani Province of Iraq
Authors: Rashid Shorsh Gharib , Sulaiman Rizgar Raheem , Saeed Nahla Mohammed 
Published: March 2023

Abstract
Background

Mycobacterium bovis (M. bovis) causes bovine tuberculosis (BTB), is an endemic disease in cattle and poses a high risk of spreading to humans.

Objective

This study aimed to determine M. bovis in cattle and assess the similarities between cattle and humans through molecular methods and histopathological examinations.
Methodology
Randomly, blood samples from 411 healthy appearance cows (1% of the target population) in five districts in Sulaimani province, Iraq, were collected from January to March 2022. Sera were obtained immediately and used for ELISA test to determine M. bovis. Additionally, the disease prevalence was confirmed by gross lesions at the slaughterhouse and histopathological examination of collected lymph nodes. Moreover, a PCR assay was used to detect M. bovis in suspected cow samples and previously diagnosed human samples. Gene sequencing and phylogenetic tree analysis were also done to determine the molecular differences between animal and human M. bovis.

Results

Using an ELISA test, 46 (11.11%) of 414 samples were positive, while 368 (88.89%) were negative without significant differences between the districts (p > 0.05). According to postmortem lesions at the slaughterhouse, only three cows were infected with TB, and typical gross lesions were calcified necrotic and multiple well-demarcated granulomas. The molecular test using two primers (CSB2 and oxyR gene) revealed that M. bovis was found in animal and human extra-pulmonary lymph nodes with no molecular change.

Conclusion

Healthy cows harbored M. bovis, the causative agent of a contagious disease that spreads and causes a persistent health problem in humans.
IL4I1-catalyzed tryptophan metabolites mediate the anti-inflammatory function of cytokine-primed human muscle stem cells
Authors: Shao Changshun , Zuo Muqiu , Fang Jiankai , Huang Peiqing , Liu Shisong , Hou Pengbo , Wang Shiqing , Liu Zhanhong , Feng Chao , Cao Lijuan , Li Peishan , Shi Y 
Published: March 2023

Abstract
Muscle stem cells (MuSCs) have been demonstrated to exert impressive therapeutic efficacy in disease settings through orchestrating inflammatory microenvironments. Nevertheless, the mechanisms underlying the immunoregulatory property of MuSCs remain largely uncharacterized. Here, we showed that interleukin-4-induced-1 (IL4I1), an essential enzyme that catalyzes indole metabolism in humans, was highly expressed in human MuSCs exposed to IFN-γ and TNF-α. Functionally, the MuSCs were found to inhibit the infiltration of neutrophils into sites of inflammation in a IL4I1-dependent manner and thus ameliorate acute lung injury in mice. Mechanistically, the indole metabolites, including indole-3-pyruvic acid (I3P) and indole-3-aldehyde (I3A), produced by IL4I1, acted as ligands to activate aryl hydrocarbon receptor (AHR), leading to augmented expression of TNF-stimulated gene 6 (TSG-6) in inflammatory cytokine-primed MuSCs. Furthermore, I3P administration alone suppressed neutrophil infiltration in damaged lungs. I3P could also reduce the level of reactive oxygen species in neutrophils. Therefore, our study has uncovered a novel mechanism by which MuSCs acquire their immunoregulatory property and may help to develop or optimize MuSC-based therapies for inflammatory diseases.
Magnetic carbon Fe3O4 nanocomposites synthesized via Magnetic Induction Heating
Authors: Cervera-Gabalda Laura , Gómez-Polo Cristina 
Published: March 2023

Abstract
Magnetic Induction Heating (MIH) of magnetite nanoparticles is employed as a novel synthesis procedure of carbon based magnetic nanocomposites. Magnetic nanoparticles (Fe3O4) and fructose (1:2 weight ratio) were mechanically mixed and submitted to a RF magnetic field (305 kHz). The heat generated by the nanoparticles leads to the decomposition of the sugar and to the formation of an amorphous carbon matrix. Two sets of nanoparticles, with mean diameter sizes of 20 and 100 nm, are comparatively analysed. Structural (X-ray diffraction, Raman spectroscopy, Transmission Electron Microscopy (TEM)), electrical and magnetic (resistivity, SQUID magnetometry) characterizations confirm the nanoparticle carbon coating through the MIH procedure. The percentage of the carbonaceous fraction is suitably increased controlling the magnetic heating capacity of the magnetic nanoparticles. The procedure enables the synthesis of multifunctional nanocomposites with optimized properties to be applied in different technological fields. Particularly, Cr (VI) removal from aqueous media is presented employing the carbon nanocomposite with 20 nm Fe3O4 nanoparticles.
The belowground mechanisms of phosphite-mediated protection against root rot disease in Rhododendron species
Authors: Liu Yu , Burke David J. , Medeiros Juliana S. , Carrino-Kyker Sarah R. , Burns Jean H. 
Published: March 2023

Abstract
Background and Aims
The soil-borne pathogen Phytophthora cinnamomi causes a deadly plant disease. Phosphite is widely used as an effective treatment to protect plants from Phytophthora cinnamomi. Phosphite as a common fungicide might influence the composition of soil fungal communities. However, whether the belowground mechanisms of phosphite-mediated protections are direct or indirectly mediated through soil biota are unknown. Therefore, exploring belowground mechanisms could contribute to the evaluation of the sustainability of phosphite use and tests hypotheses about direct versus indirect mechanisms in pathogen response.
Methods
Our greenhouse pot experiment on Rhododendron species had either an after-pathogen or a before-pathogen use of phosphite to compare and evaluate plant and soil fungal responses to phosphite and the presence of an oomycete pathogen phytophthora cinnamomi. The factorial experiment also included with and without pathogen and soil biota treatments, for a test of interactive effects. High throughput sequencing analyzed the soil fungal communities, and we measured the diversity, evenness and richness of soil fungi.
Results
Phosphite effectively increased survival of Rhododendron species. It altered the composition of soil fungal communities, and the timing of using phosphite determined the way in which the fungal communities changed. Trichoderma taxa also responded to soil phosphite and Phytophthora cinnamomi.
Conclusions
The benefits of antagonistic fungi such as Trichoderma are context-dependent, suggesting protection against pathogens depends on the timing of phosphite application. This study provides the first evidence that phosphite-mediated pathogen protection includes both direct benefits to plants and indirect effects mediated through the soil microbial community.
Dynamic Full-Field Optical Coherence Tomography module adapted to commercial microscopes for longitudinal in vitro cell culture study
Authors: Monfort Tual , Azzollini Salvatore , Brogard Jeremy , Clémençon Marilou , Brec Amélie Slembrouck , Forster Valérie , Picaud Serge , Goureau Olivier  , Reichman Sacha , Thouvenin Olivier , Grieve Kate 
Published: March 2023

Abstract
Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Here, a D-FFOCT module design is presented which can be coupled to a commercial microscope with a stage top incubator, allowing non-invasive label-free longitudinal imaging over periods of minutes to weeks on the same sample. Long term volumetric imaging on human induced pluripotent stem cell-derived retinal organoids is demonstrated, highlighting tissue and cell organisation as well as cell shape, motility and division. Imaging on retinal explants highlights single 3D cone and rod structures. An optimal workflow for data acquisition, postprocessing and saving is demonstrated, resulting in a time gain factor of 10 compared to prior state of the art. Finally, a method to increase D-FFOCT signal-to-noise ratio is demonstrated, allowing rapid organoid screening.
Temporal and spatial variation of chlorophyll a concentration and ecological environment assessment in Bohai Sea
Authors: Zhao Zihan , Xiao Rushui , Guo Junting , Zhang Yuming , Zhang Shufang , Lv Xianqing , Shi Honghua 
Published: March 2023

Abstract
Chlorophyll-a (Chl-a) is an important indicator of phytoplankton bloom and water eutrophication, as well as a basic parameter to assess coastal ecosystem health. This study utilizes a significant amount of observational data regarding Chl-a concentration. The linear Radial Basis Function (RBF-Linear) is a fitting method used to reconstruct the in-situ observation data of Chl-a concentration in the Bohai Sea in August 2017, with two advantages of small comprehensive error and reasonable spatial distribution. This method is selected through 10-fold cross-validation, correlation analysis and spatial distribution comparison. This will provide a potential application prospect for evaluating satellite products and improving the accuracy of ecological models. Then we use this method to obtain the complete three-dimensional spatial field of Chl-a concentration in the Bohai Sea during March, May, August and October from 2016 to 2018. The spatial and temporal distribution of Chl-a concentration in the Bohai Sea shows obvious spatial and temporal distribution characteristics: spatially, the high Chl-a concentration is mainly distributed in coastal waters, especially near estuaries and mariculture area; Temporally, there are two obvious peaks in March and August. Additionally, two indicators of total Chl-a and area with high Chl-a concentration are given in order to assess the marine ecological environment in four sub-regions of the Bohai Sea (Bohai Bay, Liaodong Bay, Laizhou Bay, and Central Bohai Sea), with a focus on the impacts of special weather events and bottom water quality. Along with restoring the marine natural environment, attention should be given to balancing urban development.
Very-early-onset inflammatory bowel disease with a partial RIPK1/ BPHL deletion in an infant
Authors: Kırsaçlıoğlu Ceyda Tuna , Frohne Alexandra , Kuloğlu Zarife , Demir Engin , Altuntaş Cansu , Haskoloğlu Zehra Şule , Çobanoğlu Fatma Nazan , Kendirli Tanıl , Özdemir Halil , Özçakar Zeynep Birsin , Savaş Berna , Doğu Figen , İkincioğulları Aydan , Boztuğ Kaan , Kansu Aydan 
Published: March 2023

Abstract

The monogenic causes of very early-onset inflammatory bowel disease (VEO-IBD) have been defined by advanced genetic studies, which were usually related to primary immunodeficiencies. Receptor-interacting serine/threonine-protein kinase-1 RIPK-1 protein is an important signalling molecule in inflammation and cell death pathways. Its deficiency may lead to different clinical features, via immunodeficiency and/or inflammation. Here we discussed an infant with malnutrition, VEO-IBD, reccurrent infections and polyathritis, who had a partial deletion in RIPK1 gene, adjacent biphenyl hydrolase-like (BPHL) gene, and a homozygous p.Glu148Gln mutation in Mediterranean fever (MEFV) gene. The superimposing effect of immune dysregulation of these two defective genes may lead to severe clinical presentation of our patient.
Quantitative and qualitative assessment to predict fall events for older inpatients at a tertiary referral hospital
Authors: Komiya Hitoshi , Imaizumi Takahiro , Suzuki Yusuke , Ando Masahiko , Shiratori Yoshimune , Nagao Yoshimasa , Kuzuya Masafumi 
Published: March 2023

Abstract
Purpose
Falls among older patients are one of the most common adverse events in hospitals. Many hospitals have established and adopted their own multifaceted fall assessment tool. There has not been much research on fall assessment tools’ validation by incorporating various factors as covariates. We aimed to validate a fall risk assessment tool for older inpatients who are admitted to a tertiary referral hospital.
Methods
This study used electronic medical record (EMR) of inpatients aged ≥60 years who were admitted to Nagoya University Hospital from January 1st, 2020 to December 31st, 2020 were included in the study. On admission, the patients’ background information and fall risk assessment were extracted retrospectively from the EMR. Fall events within 30 days of hospitalization were the outcome, which were prospectively recorded by the nurses. We examined the association between fall risk assessment scores and the outcome as well as the C-statistics to examine if adding the score improved the models.
Results
A total of 10,353 patients were included in the study. The number of fall event was 224. The hazard ratio (95% confidence interval) for the fall risk assessment score was 1.30 (1.18–1.45) in multivariable Cox models. By adding the risk assessment scores, C-statistics of the multivariable models improved by 0.031 (0.007–0.054).
Conclusions
Even after adjusting for numerous quantitative factors, we validated the usefulness of the risk assessment tool in predicting fall events, demonstrating the utility of a qualitative tool assessed by healthcare providers in predicting fall events.
Global patterns of thermal niche filling in ectotherms
Authors: Moore Nikki , Morales-Castilla Ignacio , Hargreaves Anna , Olalla-Tárraga Miguel , Villalobos Fabricio , Calosi Piero , Clusella-Trullas Susana , Rubalcaba Juan , Algar Adam , Martínez Brezo , Rodríguez Laura , Gravel Sarah , Bennett Joanne , Carrete-Vega Greta , Rahbek Carsten , Araújo Miguel , Bernhardt Joey , Sunday Jennifer 
Published: March 2023

Abstract
Understanding how temperature determines the distribution of life is necessary to assess species’ sensitivities to contemporary climate change. Here we test the importance of temperature in limiting geographic ranges of ectotherms by comparing temperatures across occupied ranges to those species could potentially occupy based on their physiological thermal tolerances. Whereas marine and tropical terrestrial species occupy temperatures that closely match their thermal tolerances, high-latitude terrestrial species under-occupy warm temperatures and are absent from thermally tolerable areas towards the equator. This suggests that on land, temperature less often limits the equatorward range edge of temperate species, supporting the hypothesis that their historic expansion ‘out of the tropics’ was associated with tropical biotic exclusion. Our findings predict more direct responses to climate warming of marine ranges and cool range edges of terrestrial species.
Liquid chromatography as analytical tool for the study of natural and early synthetic dyes in traditional Saxon textiles
Authors: Petroviciu Irina , Teodorescu Iulia , Vasilca Silvana , Albu Florin , Medvedovici Andrei 
Published: March 2023

Abstract
Confirmed since the 12-th century, the Saxon community in Transylvania developed over the years in a rigorous powerful society, with its own lifestyle, social, economic, cultural and artistic standards. Together with research in historic documents, this society is now revealed by material studies of 18-th to 20-th century objects in the Emil Sigerius collection, preserved in the ASTRA Museum, Sibiu. Embroideries made in Saxon households as well as representative Saxon coats manufactured in workshops and dated between 1892 and 1908 (years embroidered on objects) were studied in terms of dye analysis in order to understand if adoption of new materials goes together with the stylistic improvements. Dye analysis were performed by liquid chromatography with UV-Vis and mass spectrometric detection (LC-DAD-MS), nowadays the most appropriate technique for the characterization and identification of colour components in heritage textiles. MS/MS detection, in its tri-quadrupolar architecture, with the middle quadrupole used as collision cell, was used to achieve undoubted identification of each dye in a complex matrix. Construction of suitable spectral libraries through studies on standard dyes and dyed textiles definitely improved the ability to undoubtedly detect natural and early synthetic dyes. Identification of fuchsine (1856), methyl violet (1861), orange II (1876) brilliant green (1879), quinoline yellow (1881) and rhodamine B (1887) in textiles dated about 20 years after the dyes first synthesis prove that transition from natural to synthetic dyes in the Saxon community was very fast, especially in what textiles made in specialized workshops are concerned. The analytical technique used for the natural and early synthetic dyes characterisation and identification, and especially the triple quadrupole mass spectrometer with its possibility for tuning the selectivity through appropriately exploiting the functioning modes, as well as to increase sensitivity, confirms its ability in studies aiming in understanding textiles through dye analysis and promotes the use of advanced analytical techniques in heritage science applications.
A Single Camera Unit-based Three-Dimensional Surface Imaging Technique
Authors: Wang Yinhe , Guo Xiangyu , Kim Jungsub , Lin Pengfei , Lu Kuan , Lee Hyunjae , Lee ChaBum 
Published: March 2023

Abstract
This paper introduces a simple three-dimensional (3D) stereoscopic method using a single unit of an imaging device consisting of a charge-coupled device (CCD) and a zoom lens. Unlike conventional stereoscopy, which requires a pair of imaging devices, 3D surface imaging is achieved by 3D image reconstruction of two images obtained from two different camera positions by scanning. The experiments were performed by obtaining two images of the measurement target in two different ways: (1) by moving the object while the imaging device is stationary, and (2) by moving the imaging device while the object is stationary. Conventional stereoscopy is limited by disparity errors in 3D image reconstruction because a pair of imaging devices is not ideally identical and alignment errors are always present in the imaging system setup. The proposed method significantly reduced the disparity error in 3D image reconstruction, and the calibration process of the imaging system became simple and convenient. The proposed imaging system showed a disparity error of 0.26 in the camera pixel.