bluuebunny/crossref_metadata_2025_split huggingface dataset:
Life satisfaction across the entire trajectory of Alzheimer's disease: A mediation analysis
Authors: Mank Arenda , van Maurik Ingrid S. , van Harten Argonde C. , Rhodius‐Meester Hanneke F. M. , Teunissen Charlotte E. , van Berckel Bart N. M. , Berkhof Johannes , van der Flier Wiesje M. , Rijnhart Judith J. M. 
Published: December 2022

AbstractIntroductionWe studied life satisfaction across Alzheimer's disease (AD) stages and studied mobility and meaningful activities as mediators of the associations between these AD stages and life satisfaction.MethodsIn this cross‐sectional study, we included n = 269 amyloid‐positive patients with subjective cognitive decline (SCD), mild cognitive impairment (MCI), and AD dementia from the Amsterdam Dementia Cohort. Life satisfaction was measured with the satisfaction with life scale. The mediating role of transportation, work, sports, and hobbies on life satisfaction was examined in single and multiple mediator models.ResultsPatients with dementia are less satisfied with life compared to SCD and MCI. These differences in life satisfaction are explained by reduced participation in meaningful activities, which in turn, was largely attributable to decreased transportation use.DiscussionOur findings suggest that improving access to transportation, therewith allowing participation in meaningful activities help to maintain life satisfaction and may be an important target for intervention.
Dyslipidemia and SARS‐CoV‐2 spike antibody titres after the second and third doses of the BNT162b2 vaccine among healthcare workers in Japan
Authors: Islam Zobida , Yamamoto Shohei , Mizoue Tetsuya , Oshiro Yusuke , Inamura Natsumi , Konishi Maki , Ozeki Mitsuru , Sugiura Wataru , Ohmagari Norio 
Published: December 2022

AbstractObjectiveThis study aimed to examine the sex‐associated differences in the relationship between dyslipidemia and severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike immunoglobulin (Ig)G antibodies among BNT162b2 vaccine recipients.MethodsParticipants were staff members (aged 21–75 years) of a medical and research institution who underwent an anti‐SARS‐CoV‐2 spike IgG antibody test after the second (n = 1872) and third doses (n = 1075) of the BNT162b2 vaccine. Dyslipidemia was defined as triglyceride level ≥150 mg/dl, high‐density lipoprotein‐cholesterol level <40 mg/dl, low‐density lipoprotein‐cholesterol level ≥140 mg/dl, or lipid‐lowering medication use. Multivariable linear regression was used to calculate the ratio of means for SARS‐CoV‐2 spike IgG titre according to dyslipidemia status.ResultsThe prevalence of dyslipidemia was 38.0% in men and 19.6% in women. The relationship between dyslipidemia and SARS‐CoV‐2 spike IgG titres after the second dose differed markedly by sex (P for interaction <0.001). In men, dyslipidemia was associated with significantly lower IgG titres: the ratio of means (95% confidence interval) was 0.82 (0.72–0.93). However, this association disappeared after the third dose (0.96 [0.78–1.18]). Of the dyslipidemia components, hypertriglyceridemia was inversely associated with SARS‐CoV‐2 spike IgG antibody titre after both the second and third doses (ratio of means: 0.82 [0.70–0.95] and 0.73 [0.56–0.95], respectively). In women, IgG titres did not differ according to dyslipidemia or hypertriglyceridemia status after either dose.ConclusionsThese results suggest a detrimental role of hypertriglyceridemia in the humoral immune response to the BNT162b2 vaccine for COVID‐19 in men but not in women.
Models combining multiple scales of inference capture hydrologic and climatic drivers of riparian tree distributions
Authors: Perry Laura G. , Jarnevich Catherine S. , Shafroth Patrick B. 
Published: December 2022

AbstractPredicting species geographic distributions is key to managing invasive species, conserving biodiversity, and understanding species' environmental requirements. Species distribution models (SDMs) commonly focus on climatic predictors, but other environmental factors can also be essential, particularly for species with specialized habitats defined by hydrologic, topographic, or edaphic conditions (e.g., riparian, wetland, alpine, coastal, serpentine). Here, we demonstrate a novel approach for capturing strong effects of both hydrologic and climatic predictors in SDMs for riparian plants, by merging analyses targeted at environmental drivers within riparian ecosystems and across the western USA (3.8 × 106 km2). We developed presence‐background SDMs from five algorithms for three invasive riparian trees (Tamarix ramossisima/chinensis [saltcedar], Elaeagnus angustifolia [Russian olive], and Ulmus pumila [Siberian elm]) and three native Populus spp. (cottonwoods). We used separate background datasets to develop models with different spatial scales of inference: (1) spatially filtered random points to represent available habitat across the study area and (2) target‐group points from Salix (willow) occurrences to represent available riparian habitat. Random‐background models captured hydrologic drivers of riparian tree distributions relative to the largely upland western USA, whereas Salix‐background models captured climatic drivers within the context of riparian ecosystems. Combining predictions from the two backgrounds identified hydrologically suitable habitats within climatically suitable regions, resulting in fewer false “absences” than either background alone, improving predictions over previous SDMs, and providing more complete information to guide management decisions. Surprisingly, the predicted habitat for U. pumila, a newly recognized riparian invader, was as or more extensive than Populus deltoides/fremontii, T. ramossisima/chinensis, and E. angustifolia, the most common riparian tree complexes in the western USA. Watersheds constituting 20% of U. pumila predicted habitat contained no occurrence records, indicating high risk of future and unrecognized invasions. Combining models from random and ecosystem‐specific target‐group backgrounds may improve SDMs for species from many specialized habitats, providing a method to link predicted distributions to localized geographic features while capturing broad‐scale climatic requirements.
Guidelines for mouse and human DC functional assays
Authors: Clausen Björn E. , Amon Lukas , Backer Ronald A. , Berod Luciana , Bopp Tobias , Brand Anna , Burgdorf Sven , Chen Luxia , Da Meihong , Distler Ute , Dress Regine J. , Dudziak Diana , Dutertre Charles‐Antoine , Eich Christina , Gabele Anna , Geiger Melanie , Ginhoux Florent , Giusiano Lucila , Godoy Gloria J. , Hamouda Ahmed E.I. , Hatscher Lukas , Heger Lukas , Heidkamp Gordon F. , Hernandez Lola C. , Jacobi Lukas , Kaszubowski Tomasz , Kong Wan Ting , Lehmann Christian H. K. , López‐López Tamara , Mahnke Karsten , Nitsche Dominik , Renkawitz Jörg , Reza Rifat A. , Sáez Pablo J. , Schlautmann Laura , Schmitt Madeleine T. , Seichter Anna , Sielaff Malte , Sparwasser Tim , Stoitzner Patrizia , Tchitashvili Giorgi , Tenzer Stefan , Tochoedo Nounagnon R. , Vurnek Damir , Zink Fabian , Hieronymus Thomas 
Published: December 2022

AbstractThis article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non‐lymphoid tissues. Recent studies have provided evidence for an increasing number of phenotypically distinct conventional DC (cDC) subsets that on one hand exhibit a certain functional plasticity, but on the other hand are characterized by their tissue‐ and context‐dependent functional specialization. Here, we describe a selection of assays for the functional characterization of mouse and human cDC. The first two protocols illustrate analysis of cDC endocytosis and metabolism, followed by guidelines for transcriptomic and proteomic characterization of cDC populations. Then, a larger group of assays describes the characterization of cDC migration in vitro, ex vivo, and in vivo. The final guidelines measure cDC inflammasome and antigen (cross)‐presentation activity. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer‐reviewed by leading experts and approved by all co‐authors, making it an essential resource for basic and clinical DC immunologists.
Staphylococcus aureus‐derived factors promote human Th9 cell polarization and enhance a transcriptional program associated with allergic inflammation
Authors: Badolati Isabella , van der Heiden Marieke , Brodin David , Zuurveld Marit , Szilágyi Szilvia , Björkander Sophia , Sverremark‐Ekström Eva 
Published: December 2022

AbstractT helper (Th) 9 cells, characterized by robust secretion of IL‐9, have been increasingly associated with allergic diseases. However, whether and how Th9 cells are modulated by environmental stimuli remains poorly understood. In this study, we show that in vitro exposure of human PBMCs or isolated CD4 T‐cells to Staphylococcus (S.) aureus‐derived factors, including its toxins, potently enhances Th9 cell frequency and IL‐9 secretion. Furthermore, as revealed by RNA sequencing analysis, S. aureus increases the expression of Th9‐promoting factors at the transcriptional level, such as FOXO1, miR‐155, and TNFRSF4. The addition of retinoic acid (RA) dampens the Th9 responses promoted by S. aureus and substantially changes the transcriptional program induced by this bacterium, while also altering the expression of genes associated with allergic inflammation. Together, our results demonstrate a strong influence of microbial and dietary factors on Th9 cell polarization, which may be important in the context of allergy development and treatment.
Non‐synonymous, synonymous, and non‐coding nucleotide variants contribute to recurrently altered biological processes during retinoblastoma progression
Authors: Stachelek Kevin , Harutyunyan Narine , Lee Susan , Beck Assaf , Kim Jonathan , Xu Liya , Berry Jesse L. , Nagiel Aaron , Reynolds C. Patrick , Murphree A. Linn , Lee Thomas C. , Aparicio Jennifer G. , Cobrinik David 
Published: December 2022

AbstractRetinoblastomas form in response to biallelic RB1 mutations or MYCN amplification and progress to more aggressive and therapy‐resistant phenotypes through accumulation of secondary genomic changes. Progression‐related changes include recurrent somatic copy number alterations and typically non‐recurrent nucleotide variants, including synonymous and non‐coding variants, whose significance has been unclear. To determine if nucleotide variants recurrently affect specific biological processes, we identified altered genes and over‐represented variant gene ontologies in 168 exome or whole‐genome‐sequenced retinoblastomas and 12 tumor‐matched cell lines. In addition to RB1 mutations, MYCN amplification, and established retinoblastoma somatic copy number alterations, the analyses revealed enrichment of variant genes related to diverse biological processes including histone monoubiquitination, mRNA processing (P) body assembly, and mitotic sister chromatid segregation and cytokinesis. Importantly, non‐coding and synonymous variants increased the enrichment significance of each over‐represented biological process term. To assess the effects of such mutations, we examined the consequences of a 3′ UTR variant of PCGF3 (a BCOR‐binding component of Polycomb repressive complex I), dual 3′ UTR variants of CDC14B (a regulator of sister chromatid segregation), and a synonymous variant of DYNC1H1 (a regulator of P‐body assembly). One PCGF3 and one of two CDC14B 3′ UTR variants impaired gene expression whereas a base‐edited DYNC1H1 synonymous variant altered protease sensitivity and stability. Retinoblastoma cell lines retained only ~50% of variants detected in tumors and enriched for new variants affecting p53 signaling. These findings reveal potentially important differences in retinoblastoma cell lines and tumors and implicate synonymous and non‐coding variants, along with non‐synonymous variants, in retinoblastoma oncogenesis.
Structural Flexibility of Metal Chelate Complexes and Its Relation to Supramolecular Chemistry
Authors: Maverick Andrew W. 
Published: December 2022

AbstractCrystal structures of metal chelate and related complexes in the Cambridge Structural Database have been analyzed, with respect to their use as components in supramolecular metal‐organic compounds. In β‐diketonate complexes, the distribution of angles between ligands is relatively broad; other ligands, such as 2,2′‐bipyridine, yield significantly narrower distributions. According to the principle of structure correlation, these distributions reflect the ease of distorting the various families of complexes. A comparison through density functional theory calculations also indicates that angular distortions require significantly less energy for M(β‐diketonate)3 than for M(2,2′‐bipyridine)3. The differences are likely to affect the construction of supramolecular systems from different combinations of metals and ligands, including the likelihood that the desired structures will be obtained.
Epigenetics applied to child and adolescent mental health: Progress, challenges and opportunities
Authors: Cecil Charlotte A. M. , Neumann Alexander , Walton Esther 
Published: December 2022

AbstractBackgroundEpigenetic processes are fast emerging as a promising molecular system in the search for both biomarkers and mechanisms underlying human health and disease risk, including psychopathology.MethodsIn this review, we discuss the application of epigenetics (specifically DNA methylation) to research in child and adolescent mental health, with a focus on the use of developmentally sensitive datasets, such as prospective, population‐based cohorts. We look back at lessons learned to date, highlight current developments in the field and areas of priority for future research. We also reflect on why epigenetic research on child and adolescent mental health currently lags behind other areas of epigenetic research and what we can do to overcome existing barriers.ResultsTo move the field forward, we advocate for the need of large‐scale, harmonized, collaborative efforts that explicitly account for the time‐varying nature of epigenetic and mental health data across development.ConclusionWe conclude with a perspective on what the future may hold in terms of translational applications as more robust signals emerge from epigenetic research on child and adolescent mental health.
Serum extracellular vesicle‐derived ASS1 is a promising predictor for the occurrence of HEV‐ALF
Authors: Xiang Ze , Jiang Chun , Yang Jiajia , Huang Lan , Jiang Bin , Wang Xuanli , Gao Ce , Li Mo , Meng Yiling , Tong Ling , Ling Bai , Wang Ying , Wu Jian ,   The Chinese Consortium for the Study of Hepatitis E (CCSHE)
Published: December 2022

AbstractDevelopment of biomarkers for predicting the occurrence of hepatitis E virus related‐acute liver failure (HEV‐ALF) is conducive to prevention and early intervention. Serum samples from 250 HEV‐ALF patients, 250 patients with acute hepatitis E (AHE) and 250 health controls (HCs) were collected. We assessed the predictive ability of extracellular vesicle (EV)‐derived argininosuccinate synthase 1 (ASS1) levels for HEV‐ALF occurrence. Serum EVs were successfully isolated. EV‐derived ASS1 levels in the HEV‐ALF patients were significantly higher than those in the AHE patients and HCs. In HEV‐ALF patients, EV‐derived ASS1 levels were positively correlated with the number of failed organs and disease progression. The logistical regression showed that EV‐derived ASS1 level is an independent risk factor for HEV‐ALF, and orthogonal partial least squares discriminant analysis (OPLS‐DA) also suggested that EV‐derived ASS1 level has high predictive capability. Besides, the area under the curve (AUC) of EV‐derived ASS1 level to predict HEV‐ALF occurrence was 0.728 (0.684–0.772) with the sensitivity and specificity being 72.80% and 64.80%, which had a high decision‐making ability. Furthermore, there existed no significant difference between the age ≥60 and age <60 groups in EV‐derived ASS1 levels. Serum EV‐derived ASS1 level is a promising predictor for the occurrence of HEV‐ALF.
Am I the only one? Consequences of change championing (a)symmetry on group‐ and individual‐level change outcomes
Authors: Kanitz Rouven , Gonzalez Katerina , Berger Stefan , Reinwald Max , Huettermann Hendrik , Franczak Jennifer 
Published: December 2022

SummaryEmployee change championing (i.e., discretionary behaviors to promote change to others) is critical for implementing organizational change successfully. However, extant research has been focused on individual‐level championing without considering the broader group context in which championing occurs. Our study adopts a multilevel perspective to provide insights into the effects of change championing at both the group and the individual level. We collected data at two points in time from 267 employees in 69 groups undergoing an organizational change in a German technology company. Multilevel modeling results show that group championing asymmetry (i.e., the degree to which group members differ in their championing) weakens the positive effects of group championing level on change implementation effectiveness. Moreover, we shed light on the individual‐level processes that underpin group championing dynamics. Our findings reveal that employees who are embedded in groups with high average championing levels perceive a more positive change impact (but do not experience higher levels of enthusiasm) and report higher levels of individual championing at a later point in time compared to employees in groups with lower championing levels. Our study expands the championing literature to the group‐level and offers a multilevel perspective on the championing dynamics between individuals and groups.
Photoresponsive Adhesives Based on Arylazoisoxazoles‐Containing Polymers
Authors: Rossi Ruggero , Salvini Antonella , Pizzo Benedetto , Frediani Marco , Wiersma Diederik Sybolt , Parmeggiani Camilla , Martella Daniele 
Published: December 2022

AbstractAdhesives exist in many forms and are essential in the daily life, in industrial processes, and in healthcare, calling for continuous improvements in terms of application fields, strength, biocompatibility, recyclability, and reversibility. Regarding the last two points, embedding photoresponsive molecules into the adhesive allows the use of light to attach or detach reversibly different surfaces. Photoresponsive adhesives can be removed without the use of high temperature or solvents, which is ideal for delicate environments. To date, azobenzenes are the most explored chromophore to prepare smart adhesive formulations. Here, the synthesis of polymer containing arylazoisoxazoles (AIZs) — azobenzene analogs containing a heterocycle — and their application as adhesives is described. Mechanical tests demonstrate an enhancement of the adhesion strength from 0.39 to 2.79 MPa, passing from low molecular weight monomer to selected AIZs acrylate polymer. Irradiation with UV induces the formation of the cis isomer with a consequent drop of more than 70% in the adhesion strength. The different compounds result as possible candidates as adhesives on the basis of the increased load that can be held, together with the possibility to obtain joints that can be either stable or detachable under light irradiation.
Mesothelioma cancer cells are glutamine addicted and glutamine restriction reduces YAP1 signaling to attenuate tumor formation
Authors: Adhikary Gautam , Shrestha Suruchi , Naselsky Warren , Newland John J. , Chen Xi , Xu Wen , Emadi Ashkan , Friedberg Joseph S. , Eckert Richard L. 
Published: December 2022

AbstractGlutamine addiction is an important phenotype displayed in some types of cancer. In these cells, glutamine depletion results in a marked reduction in the aggressive cancer phenotype. Mesothelioma is an extremely aggressive disease that lacks effective therapy. In this study, we show that mesothelioma tumors are glutamine addicted suggesting that glutamine depletion may be a potential therapeutic strategy. We show that glutamine restriction, by removing glutamine from the medium or treatment with inhibitors that attenuate glutamine uptake (V‐9302) or conversion to glutamate (CB‐839), markedly reduces mesothelioma cell proliferation, spheroid formation, invasion, and migration. Inhibition of the SLC1A5 glutamine importer, by knockout or treatment with V‐9302, an SLC1A5 inhibitor, also markedly reduces mesothelioma cell tumor growth. A relationship between glutamine utilization and YAP1/TEAD signaling has been demonstrated in other tumor types, and the YAP1/TEAD signaling cascade is active in mesothelioma cells and drives cell survival and proliferation. We therefore assessed the impact of glutamine depletion on YAP1/TEAD signaling. We show that glutamine restriction, SLC1A5 knockdown/knockout, or treatment with V‐9302 or CB‐839, reduces YAP1 level, YAP1/TEAD‐dependent transcription, and YAP1/TEAD target protein (e.g., CTGF, cyclin D1, COL1A2, COL3A1, etc.) levels. These changes are observed in both cells and tumors. These findings indicate that mesothelioma is a glutamine addicted cancer, show that glutamine depletion attenuates YAP1/TEAD signaling and tumor growth, and suggest that glutamine restriction may be useful as a mesothelioma treatment strategy.
Improved seasonal definition and projected future seasons in South Korea
Authors: Lee Eungul , Im Ahyeong , Oh Jieun , Song Miseon , Choi Youngeun , Choi Dasom 
Published: December 2022

AbstractSeasons are fundamental climatological phenomena, and their changes have significantly affected the sustainability of nature and human society. Suitable criteria to define the seasonal indicators of start date and length of seasons are required for the timely planning of socio‐economic activities. However, unsuitable cases of seasonal indicators have been identified in the seasonal definition used by Korea Meteorological Administration (KMA). We devised the data processing method to calculate three seasonal indicators: start date, length, and abnormal days of seasons. The past (1921–2010) and future (2021–2100) climate data of daily mean temperature were used in estimating the three seasonal indicators; they were obtained from the weather stations in South Korea and under the Representative Concentration Pathway (RCP) scenarios, respectively. The modified definitions with the moving averages of 15‐, 31‐, 45‐, 61‐, and 91‐day improved in estimating the start date and length of seasons compared with the current KMA definition. Additionally, abnormal days of seasons in the modified definitions were found to reduce significantly. Under the RCP8.5 scenario, the earlier advance of spring and summer by 4.9 and 3.9 days per decade, respectively, and the delayed autumn and winter by 3.2 and 2.0 days per decade, respectively, were expected over the 21st century. Correspondingly, the extended summer and the shortened winter by 7.1 days per decade and 5.1 days per decade were projected, respectively. The estimated future changes in seasons using the improved seasonal definition can be used for socio‐economic sectors and the general public to adapt to a warmer climate.