bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA XIST facilitates hypertrophy of ligamentum flavum by activating VEGFA-mediated autophagy through sponging miR-302b-3p
Authors: Cao Yanlin , Li Jianjun , Qiu Sujun , Ni Songjia , Duan Yang 
Published: May 2023

Abstract
Background
Increasing evidences have shown that long non-coding RNAs (lncRNAs) display crucial regulatory roles in the occurrence and development of numerous diseases. However, the function and underlying mechanisms of lncRNAs in hypertrophy of ligamentum flavum (HLF) have not been report.

Methods
The integrated analysis of lncRNAs sequencing, bioinformatics analysis and real-time quantitative PCR were used to identify the key lncRNAs involved in HLF progression. Gain- and loss-function experiments were used to explore the functions of lncRNA X inactive specific transcript (XIST) in HLF. Mechanistically, bioinformatics binding site analysis, RNA pull-down, dual-luciferase reporter assay, and rescue experiments were utilized to investigate the mechanism by which XIST acts as a molecular sponge of miR-302b-3p to regulate VEGFA-mediated autophagy.

Results
We identified that XIST was outstandingly upregulated in HLF tissues and cells. Moreover, the up-regulation of XIST strongly correlated with the thinness and fibrosis degree of LF in LSCS patients. Functionally, knockdown of XIST drastically inhibited proliferation, anti-apoptosis, fibrosis and autophagy of HLF cells in vitro and suppressed hypertrophy and fibrosis of LF tissues in vivo. Intestinally, we uncovered that overexpression of XIST significantly promoted proliferation, anti-apoptosis and fibrosis ability of HLF cells by activating autophagy. Mechanistic studies illustrated that XIST directly medullated the VEGFA-mediated autophagy through sponging miR-302b-3p, thereby enhancing the development and progression of HLF.

Conclusion
Our findings highlighted that the XIST/miR-302b-3p/VEGFA-mediated autophagy axis is involved in development and progression of HLF. At the same time, this study will complement the blank of lncRNA expression profiles in HLF, which laid the foundation for further exploration of the relationship between lncRNAs and HLF in the future.
WONDER-01: immediate necrosectomy vs. drainage-oriented step-up approach after endoscopic ultrasound-guided drainage of walled-off necrosis—study protocol for a multicentre randomised controlled trial
Authors: Sato Tatsuya , Saito Tomotaka , Takenaka Mamoru , Iwashita Takuji , Shiomi Hideyuki , Fujisawa Toshio , Hayashi Nobuhiko , Iwata Keisuke , Maruta Akinori , Mukai Tsuyoshi , Masuda Atsuhiro , Matsubara Saburo , Hamada Tsuyoshi , Inoue Tadahisa , Ohyama Hiroshi , Kuwatani Masaki , Kamada Hideki , Hashimoto Shinichi , Shiratori Toshiyasu , Yamada Reiko , Kogure Hirofumi , Ogura Takeshi , Nakahara Kazunari , Doi Shinpei , Chinen Kenji , Isayama Hiroyuki , Yasuda Ichiro , Nakai Yousuke ,   for the WONDERFUL study group in Japan, collaborators
Published: May 2023

Abstract
Background
With the increasing popularity of endoscopic ultrasound (EUS)-guided transmural interventions, walled-off necrosis (WON) of the pancreas is increasingly managed via non-surgical endoscopic interventions. However, there has been an ongoing debate over the appropriate treatment strategy following the initial EUS-guided drainage. Direct endoscopic necrosectomy (DEN) removes intracavity necrotic tissue, potentially facilitating early resolution of the WON, but may associate with a high rate of adverse events. Given the increasing safety of DEN, we hypothesised that immediate DEN following EUS-guided drainage of WON might shorten the time to WON resolution compared to the drainage-oriented step-up approach.

Methods
The WONDER-01 trial is a multicentre, open-label, superiority, randomised controlled trial, which will enrol WON patients aged ≥ 18 years requiring EUS-guided treatment in 23 centres in Japan. This trial plans to enrol 70 patients who will be randomised at a 1:1 ratio to receive either the immediate DEN or drainage-oriented step-up approach (35 patients per arm). In the immediate DEN group, DEN will be initiated during (or within 72 h of) the EUS-guided drainage session. In the step-up approach group, drainage-based step-up treatment with on-demand DEN will be considered after 72–96 h observation. The primary endpoint is time to clinical success, which is defined as a decrease in a WON size to ≤ 3 cm and an improvement of inflammatory markers (i.e. body temperature, white blood cell count, and C-reactive protein). Secondary endpoints include technical success, adverse events including mortality, and recurrence of the WON.

Discussion
The WONDER-01 trial will investigate the efficacy and safety of immediate DEN compared to the step-up approach for WON patients receiving EUS-guided treatment. The findings will help us to establish new treatment standards for patients with symptomatic WON.

Trial registration
ClinicalTrials.gov NCT05451901, registered on 11 July 2022. UMIN000048310, registered on 7 July 2022. jRCT1032220055, registered on 1 May 2022.
Diagnostic performance of standardized typical CT findings for COVID-19: a systematic review and meta-analysis
Authors: Nam Bo Da , Hong Hyunsook , Yoon Soon Ho 
Published: May 2023

AbstractObjectiveTo meta-analyze diagnostic performance measures of standardized typical CT findings for COVID-19 and examine these measures by region and national income.MethodsMEDLINE and Embase were searched from January 2020 to April 2022 for diagnostic studies using the Radiological Society of North America (RSNA) classification or the COVID-19 Reporting and Data System (CO-RADS) for COVID-19. Patient and study characteristics were extracted. We pooled the diagnostic performance of typical CT findings in the RSNA and CO-RADS systems and interobserver agreement. Meta-regression was performed to examine the effect of potential explanatory factors on the diagnostic performance of the typical CT findings.ResultsWe included 42 diagnostic performance studies with 6777 PCR-positive and 9955 PCR-negative patients from 18 developing and 24 developed countries covering the Americas, Europe, Asia, and Africa. The pooled sensitivity was 70% (95% confidence interval [CI]: 65%, 74%;I2 = 92%), and the pooled specificity was 90% (95% CI 86%, 93%;I2 = 94%) for the typical CT findings of COVID-19. The sensitivity and specificity of the typical CT findings did not differ significantly by national income and the region of the study (p > 0.1, respectively). The pooled interobserver agreement from 19 studies was 0.72 (95% CI 0.63, 0.81;I2 = 99%) for the typical CT findings and 0.67 (95% CI 0.61, 0.74;I2 = 99%) for the overall CT classifications.ConclusionThe standardized typical CT findings for COVID-19 provided moderate sensitivity and high specificity globally, regardless of region and national income, and were highly reproducible between radiologists.Critical relevance statementStandardized typical CT findings for COVID-19 provided a reproducible high diagnostic accuracy globally.Key pointsStandardized typical CT findings for COVID-19 provide high sensitivity and specificity.Typical CT findings show high diagnosability regardless of region or income.The interobserver agreement for typical findings of COVID-19 is substantial.Graphical abstract
Acute abdominal pain in women of reproductive age: keys to suggest a complication of endometriosis
Authors: Coutureau Juliette , Mandoul Caroline , Verheyden Cecile , Millet Ingrid , Taourel Patrice 
Published: May 2023

AbstractAlthough endometriosis is a common gynecological condition in women of reproductive age, a complication of endometriosis is rarely considered as the differential diagnosis of acute abdominal pain in that context. However, acute events in women with endometriosis can represent life-threatening conditions, which require emergent treatment and often surgical management. Mass effect of endometriotic implants can give rise to obstructive complications, specifically occurring in the bowel or in the urinary tract, while inflammatory mediators released by ectopic endometrial tissue can lead to inflammation of the surrounding tissues or to superinfection of the endometriotic implants. Magnetic resonance imaging is the best imaging modality to reach the diagnosis of endometriosis, but an accurate diagnosis is possible on computed tomography, especially in the presence of stellar, mildly enhanced, infiltrative lesions in suggestive areas. The aim of this pictorial review is to provide an image-based overview of key findings for the diagnosis of acute abdominal complications of endometriosis.
Graphical Abstract
Short-term PET-derived kinetic estimation for the diagnosis of hepatocellular carcinoma: a combination of the maximum-slope method and dual-input three-compartment model
Authors: Wang Tao , Li Boqiao , Shi Hong , Li Pengfei , Deng Yinglei , Wang Siyu , Luo Qiao , Xv Dongdong , He Jianfeng , Wang Shaobo 
Published: May 2023

Abstract
Background
Kinetic estimation provides fitted parameters related to blood flow perfusion and fluorine-18-fluorodeoxyglucose (18F-FDG) transport and intracellular metabolism to characterize hepatocellular carcinoma (HCC) but usually requires 60 min or more for dynamic PET, which is time-consuming and impractical in a busy clinical setting and has poor patient tolerance.

Methods
This study preliminarily evaluated the equivalence of liver kinetic estimation between short-term (5-min dynamic data supplemented with 1-min static data at 60 min postinjection) and fully 60-min dynamic protocols and whether short-term 18F-FDG PET-derived kinetic parameters using a three-compartment model can be used to discriminate HCC from the background liver tissue. Then, we proposed a combined model, a combination of the maximum-slope method and a three-compartment model, to improve kinetic estimation.

Results
There is a strong correlation between the kinetic parameters K1 ~ k3, HPI and $${{\varvec{V}}}_{{\varvec{b}}}$$

V

b

in the short-term and fully dynamic protocols. With the three-compartment model, HCCs were found to have higher k2, HPI and k3 values than background liver tissues, while K1, k4 and $${{\varvec{V}}}_{{\varvec{b}}}$$

V

b

values were not significantly different between HCCs and background liver tissues. With the combined model, HCCs were found to have higher HPI, K1 and k2, k3 and $${{\varvec{V}}}_{{\varvec{b}}}$$

V

b

values than background liver tissues; however, the k4 value was not significantly different between HCCs and the background liver tissues.

Conclusions
Short-term PET is closely equivalent to fully dynamic PET for liver kinetic estimation. Short-term PET-derived kinetic parameters can be used to distinguish HCC from background liver tissue, and the combined model improves the kinetic estimation.

Clinical relevance statement
Short-term PET could be used for hepatic kinetic parameter estimation. The combined model could improve the estimation of liver kinetic parameters.

Graphical Abstract
Differentiation of placenta percreta through MRI features and diffusion-weighted magnetic resonance imaging
Authors: Li Hang , Lu Tao , Li Mou , Wang Yishuang , Zhang Feng , Yuan Yi , Zhu Meilin , Zhao Xinyi 
Published: May 2023

Abstract
Objectives
To identify whether parameters measured from diffusion kurtosis and intravoxel incoherent motion help diagnose placenta percreta.

Methods
We retrospectively enrolled 75 patients with PAS disorders including 13 patients with placenta percreta and 40 patients without PAS disorders. Each patients underwent diffusion-weighted imaging (DWI), intravoxel incoherent motion (IVIM), and diffusion kurtosis imaging (DKI). The apparent diffusion coefficient (ADC), perfusion fraction (f), pure diffusion coefficient (D), pseudo-diffusion coefficient (D*), mean diffusion kurtosis (MK) and mean diffusion coefficient (MD) were measured by the volumetric analysis and compared. MRI features were also analyzed and compared. The receiver operating characteristic (ROC) curve and logistic regression analysis were used to evaluate the diagnostic efficiency of different diffusion parameters and MRI features for distinguishing placental percreta.

Results
D* was an independent risk factor from DWI for predicting placenta percreta with sensitivity of 73% and specificity of 76%. Focal exophytic mass remained as independent risk factor from MRI features for predicting placenta percreta with sensitivity of 72.7% and specificity of 88.1%. When the two risk factors were combined together, the AUC was the highest, 0.880 (95% CI 0.8–0.96).

Conclusion
D* and focal exophytic mass were associated with placenta percreta. A combination of the 2 risk factors can be used to predict placenta percreta.

Critical relevance statement
A combination of D* and focal exophytic mass can be used to differentiate placenta percreta.
Potential advantages of genetically modified mesenchymal stem cells in the treatment of acute and chronic liver diseases
Authors: Sani Farnaz , Sani Mahsa , Moayedfard Zahra , Darayee Maryam , Tayebi Lobat , Azarpira Negar 
Published: May 2023

AbstractLiver damage caused by toxicity can lead to various severe conditions, such as acute liver failure (ALF), fibrogenesis, and cirrhosis. Among these, liver cirrhosis (LC) is recognized as the leading cause of liver-related deaths globally. Unfortunately, patients with progressive cirrhosis are often on a waiting list, with limited donor organs, postoperative complications, immune system side effects, and high financial costs being some of the factors restricting transplantation. Although the liver has some capacity for self-renewal due to the presence of stem cells, it is usually insufficient to prevent the progression of LC and ALF. One potential therapeutic approach to improving liver function is the transplantation of gene-engineered stem cells. Several types of mesenchymal stem cells from various sources have been suggested for stem cell therapy for liver disease. Genetic engineering is an effective strategy that enhances the regenerative potential of stem cells by releasing growth factors and cytokines. In this review, we primarily focus on the genetic engineering of stem cells to improve their ability to treat damaged liver function. We also recommend further research into accurate treatment methods that involve safe gene modification and long-term follow-up of patients to increase the effectiveness and reliability of these therapeutic strategies.
Mesenchymal stem/stromal cells-derived extracellular vesicles as a potentially more beneficial therapeutic strategy than MSC-based treatment in a mild metabolic osteoarthritis model
Authors: Warmink Kelly , Rios Jaqueline L. , Varderidou-Minasian Suzy , Torres-Torrillas Marta , van Valkengoed Devin R. , Versteeg Sabine , Eijkelkamp Niels , Weinans Harrie , Korthagen Nicoline M. , Lorenowicz Magdalena J. 
Published: May 2023

AbstractBackgroundMesenchymal stromal/stem cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs) hold promise as a disease modifying treatment in osteoarthritis (OA). Obesity, and its associated inflammation, contribute to OA development and metabolic OA represents a specific and significant group of the OA patient population. Given their immunomodulatory properties, MSC and MSC-EVs are especially interesting for this group of patients as a therapeutic option. Here, we were the first to compare the therapeutic efficacy of MSCs and MSC-EVs in a mild OA model taking these metabolic aspects into consideration.MethodsMale Wistar-Han rats (Crl:WI(Han) (n = 36) were fed a high fat diet for 24 weeks, with unilateral induction of OA by groove surgery after 12 weeks. Eight days after surgery rats were randomized in three treatment groups receiving MSCs, MSC-EVs or vehicle injection. Pain-associated behavior, joint degeneration, and local and systemic inflammation were measured.ResultsWe demonstrated that despite not having a significant therapeutic effect, MSC-EV treatment results in lower cartilage degeneration, less pain behaviour, osteophytosis and joint inflammation, than MSC treatment. Suggesting that MSC-EVs could be a more promising therapeutic strategy than MSCs in this mild metabolic OA model.ConclusionIn summary, we find that MSC treatment has negative effects on the joint in metabolic mild OA. This is an essential finding for the significant group of patients with metabolic OA phenotype, and might help to understand why clinical translation of MSC treatment shows varying therapeutic efficacy thus far. Our results also suggest that MSC-EV-based treatment might be a promising option for these patients, however MSC-EV therapeutic efficacy will need improvement.
Nile tilapia (Oreochromis niloticus) invasion impacts trophic position and resource use of commercially harvested piscivorous fishes in a large subtropical river
Authors: Shuai Fangmin , Li Jie , Lek Sovan 
Published: May 2023

Abstract
Background
Although freshwater ecosystems cover less than 1% of the earth's surface, they support extremely high levels of biodiversity and provide vital ecosystem services. However, due to the introduction of non-native fishes, aquatic ecosystem functioning has been altered, and in some cases, declined sharply. Quantifying the impacts of invasive species has proven problematic. In this study, we examined the relative trophic position of native piscivorous fishes to estimate the effects of invasive Nile tilapia on food webs in the downstream sections of an invaded large subtropical river, the Pearl River, China. Furthermore, we quantified how native piscivorous fish diets changed as the Nile tilapia invasion progressed.

Results
The trophic position of the widely distributed and locally important economically harvested piscivorous culter fish (Culterrecurviceps), mandarinfish (Sinipercakneri), and catfish (Pelteobagrusfulvidraco) lowered significantly in the invaded Dongjiang River compared to an uninvaded reference Beijiang River. The lower trophic position of these piscivorous fishes was reflected by a major reduction in the proportion of prey fish biomass in their diets following the Nile tilapia invasion. Small fishes in the diet of culter fish from the reference river (33% small fishes, 17% zooplankton) shifted to lower trophic level zooplankton prey in the invaded river (36% zooplankton, 25% small fish), possibly due to the presence of Nile tilapia. Additionally, small fishes in the diet of mandarinfish in the reference river (46% small fishes, 11% aquatic insects) declined in the invaded river (20% aquatic insects, 30% small fishes). Similarly, the diet of catfish from the reference river shifted from fish eggs (25% fish eggs, 25% aquatic insects) to aquatic insects in the invaded river (44% aquatic insects, 5% fish eggs).

Conclusions
The results of this study contributed to a growing body of evidence, suggesting that Nile tilapia can modify trophic interactions in invaded ecosystems. It is crucial to understand the processes outlined in this study in order to better assess non-native aquatic species, conserve the stability of freshwater ecosystems, and improve current conservation strategies in reaches of the Pearl River and other similar rivers that have experienced invasions of non-native species.
Better sleep quality and higher physical activity levels predict lower emotion dysregulation among persons with major depression disorder
Authors: Rezaie Leeba , Norouzi Ebrahim , Bratty Alexandra J. , Khazaie Habibolah 
Published: May 2023

Abstract
Background
People with Major Depression Disorders (MDD) often complain about sleep problems and experience emotion dysregulation. Prior research suggests physical activity can improve both sleep quality and emotional control. However, there is limited research on emotion regulation and the impact of physical activity and sleep in this population.

Objectives
The present study examined the relationships between sleep quality, emotion regulation, and physical activity levels among patients with MDD.

Methods
The sample consisted of 118 patients with MDD (mean age: 31.85 years) who completed questionnaires on sleep quality, physical activity, emotion regulation, and depression.

Results
Results showed that more sleep problems were associated with worse emotion dysregulation, and more physical activity was associated with fewer sleep problems and less emotion dysregulation. Furthermore, physical activity and sleep quality significantly predicted emotion dysregulation, with physical activity being the stronger predictor.

Conclusions
Results from this study suggest that individuals with MDD who are able to engage in physical activity and get better sleep could experience emotional regulation benefits.
De novo ADGRV1 variant in a patient with ictal asystole provides novel clues for increased risk of SUDEP
Authors: Ji Tuo , Downs Aaron W. , Dorris Luong , Zhong Ning 
Published: May 2023

Abstract
Background
Various cardiac and autonomic manifestations are frequently reported during seizures. Among the seizure-related arrhythmia, ictal tachycardia is the most common, followed by ictal bradycardia, with ictal asystole being the rarest. The occurrence of ictal asystole may obscure the clinical presentation and delay the diagnosis, representing a life-threatening presentation of epilepsy, with an elevated risk of sudden unexpected death in epilepsy patients (SUDEP). These cardiac abnormalities are being increasingly recognized as the key to elucidating the mechanisms of SUDEP.

Case presentation
We present a 35-year-old man with a history of focal-onset seizures with impaired consciousness since his mid-20 s. He developed different types of seizures for 2 years, described as tonic seizure and atonic seizure (drop attack). During such clinical events, he suffered from falls and cardiac arrest. However, thorough cardiac electrophysiology and imaging workup failed to reveal a cardiac etiology. Subsequent video electroencephalograph (EEG) monitoring was performed, and ictal bradycardia and ictal asystole were discovered. A cardiac pacemaker was implanted, and at 3-year follow-up, the patient did not suffer more atonic seizures, or falls. Genetic tests discovered a de novo variant of Adhesion G Protein-Coupled Receptor V1 (ADGRV1), which may provide a clue for the patient’s ictal asystole and the increased risk of SUDEP.

Conclusions
Considering the important impact of ictal bradycardia and asystole on the morbidity and potential mortality of epileptic patients, it is important to simultaneously utilize EEG and electrocardiogram to confirm the diagnosis. This case report highlights the link between the de novo variant of ADGRV1 and the ictal bradycardia/asystole phenotype and implicates the importance of genetic testing in adult epilepsy patients.