bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between Fine Motor Skill and the Use of Gadget in Pre-school Age Children
Authors: Hartati Sri Indah Yuli , Awaludin Sidik , Rahmawati Eni 
Published: March 2023

Aims: To analyze the relationship between fine motor skill and the use of gadget in pre school age children.
Methods: This study used a cross-sectional design. Sampling technique using quota sampling and the sample in this study amounted to 82 pre school age children in Purwanegara, Banyumas on March-April 2022. The adopted questionnaire used to be measured use of gadgets application and abilities acceptable fine motor be measured using Denver II. Data analyzed univariate and bivariate using Gamma Test.
Results: The univariate analysis showed that there were eight respondents (9.8%) with gadgets application in the category low, 13 respondents (15.9%) moderate, and 61 respondents (74.4%) high. Denver II results showed fine motor as much as one respondent (1.2%) in category advance, 24 respondents (29.3%) caution, 56 respondents (68.3%) delayed, and one respondent (1.2%) normal. The Gamma test showed a relationship between fine motor skill and the use of gadget in pre school age children with a p-value = 0,005 (p value<0.05).
Conclusion: There is a significant relationship between fine motor Skill and the use of gadget. Based on these results, periodic developmental screening and nursing interventions are needed to address fine motoric disorders in preschool-aged children who use gadgets, and it is expected that parents will limit the use of gadgets.
Honey for The Treatment of Diabetic Foot: A Literature Review
Authors: Wasilah Hinin , Farida Lisnawati Nur , Darni Zahri 
Published: March 2023

Background: Honey known as wound healer, but the use of honey for diabetes population is lack. Scientific studies needed to provide enough evidence to justify the benefits of honey for the treatment of diabetic foot ulcers.
Aims: To identify the benefit of honey as healing diabetic foot ulcer.
Methods: The author was used PICO question as the first step of this study, then the second step was searching the articles from several databases. The inclusion and exclusion criterias were using only honey as a diabetic foot treatment on human, randomized control trial study or quasy experimental study, published less than 10 years ago (2009 – 2019), available in English and provide full text. Meanwhile, for the exclusion criteria were literature review, systematic review or meta-analysis study, using honey as a treatment to animal such as rat and rabbit, combine honey with another treatment. By using PRISMA guideline, researchers were systematically review several articles from 4 databases.
Results: After conducted search strategy by using Boolean search strategy, 4 articles were included. The articles used different types of honey and the population ranged from 8 to 63 peoples. According to the results, majority of study showed honey is effective as wound healer and safe
Conclusion: Honey wound dressing is an option for managing diabetic foot
Recommendation: As a nurse, we can offer the honey as an effective treatment or intervention tools for targeted patients or populations
Knowledge-related Human Papillomavirus Vaccination: A Study of Indonesian Women
Authors: Mutiar Astri , Wulandari Tiara , Nurhayati Nunung , Marfuah Dewi , Noor Hayati Suci 
Published: March 2023

Aims: Human papillomavirus (HPV) is a sexually transmitted virus that can lead to various diseases, most known as cervical cancer’s etiology. HPV vaccination is one of the primary prevention strategies to reduce HPV transmission. To increase vaccination rates, it is essential that women have a good knowledge of receiving the HPV vaccine. The purpose of the study was to explore the knowledge of HPV vaccination among Indonesian women.
Methods: A cross-sectional study was conducted among 462 Indonesian women recruited by convenience sampling on March to May 2022. The adapted questionnaire of knowledge with 28 items was used with Cronbach's Alpha score estimated at 0.838. Women who are 15 to 49 years old, able to understand and speak the Indonesian language, and have a gadget were included in this study. Women who already get HPV vaccination are excluded. Univariate statistics were used to describe Indonesian women’s knowledge of HPV Vaccination.
Results: The research shown that the mean age of the respondents is 23.17± 5.065. Half of the respondent level of education is college as many as 245 people (53%). Half of the respondents are employed as many as 239 people (51.7%). The average knowledge is 16.02 with a standard deviation (SD) of 6.43, the knowledge value indicates less knowledge of HPV vaccination among women. In the item analysis, found that most respondents answer incorrectly to the question “HPV testing is done to show whether the HPV vaccine is needed?" (4.3%). Most of the respondents shown did not know answered the question "HPV vaccine requires three doses" (59.3%). Most of the reasons for refusing to be vaccinated answered that the price of the vaccine was not affordable (45.5%).
Conclusion: Women’s knowledge of the HPV vaccine was nearly low in this study. The campaign and effort to spread the information related to HPV vaccination are needed. Innovative media might be the best option to spread awareness. Studies with a large sample and broad range of area are preferable.
Understanding of penetrating resistance on an inclined plate to cohesive soil using two-dimensional discrete element method
Authors: Ge Jun , Nakashima Hiroshi , Wang Xiulun 
Published: March 2023

In order to know the effect of varied grousers on the reaction of soil, the reaction of soil by a plate at different angles of inclination using the two-dimensional discrete element method (DEM) has been investigated. Either the simulation or the experiment, a flat iron plate with 5 × 77 × 100 (mm) was used, and the inclination angle between the plate and the soil surface ranged from 0 to 60 degrees with 15-degree intervals. According to the result, the cohesive soil model introduced in the 2D DEM could successfully replicate the experimental results considering the effects of different inclination angles of the plate and the values of the root mean square error between the simulation and the experiment were found as 6.62, 36.60, 58.63, 60.84, and 66.25 N for inclination angles from 0 to 60 degrees in the horizontal direction. Moreover, the RMSE values of 127.91, 216.06, 75.88, 110.40, and 25.49 N were obtained for the vertical direction. Overall, a minimal discrepancy was observed between the results of DEM simulation and those of experiments in the vertical component of reaction force when the inclination angle of the plate was increased.
Single center experience and early outcomes of Impella 5.5
Authors: Funamoto Masaki , Kunavarapu Chandra , Kwan Michael D. , Matsuzaki Yuichi , Shah Mahek , Ono Masahiro 
Published: March 2023

BackgroundAcute decompensated heart failure (HF) and cardiogenic shock (CS) frequently are refractory to conservative treatment and require mechanical circulatory support (MCS). We report our early clinical experience and evaluate patient outcomes with the newer generation surgical Impella 5.5.MethodsSeventy patients that underwent Impella 5.5 implantation between October 2019 and December 2021 at a single center were enrolled in this study. Pre-operative characteristics, peri-operative clinical course information, and post-operative outcomes were retrospectively collected.ResultsFifty-seven (81%) patients survived to discharge, and 51 (76%) patients survived at the time of the first 30 days post-discharge visit. Thirty-one patients (44%) received Impella support for a bridge to advanced surgical heart failure therapy (transplant or durable left ventricular assist device [LVAD]), 27 (39%) cases were used for a bridge to recovery/decision and 12 (17.1%) cases was used for planned perioperative support for high-risk cardiac surgery procedure.ConclusionOur results suggest that Impella 5.5 provides favorable survival in the management of HF and CS, particularly used for a bridge to heart transplant or LVAD. Early extubation and mobilization with high flow circulatory support allowed effective tailoring of MCS approaches from peri-operative support for high-risk cardiac surgery, bridge to recovery, and to advanced surgical heart failure therapy.
Identification of copper metabolism-related subtypes and establishment of the prognostic model in ovarian cancer
Authors: Zhao Songyun , Zhang Xin , Gao Feng , Chi Hao , Zhang Jinhao , Xia Zhijia , Cheng Chao , Liu Jinhui 
Published: March 2023

BackgroundOvarian cancer (OC) is one of the most common and most malignant gynecological malignancies in gynecology. On the other hand, dysregulation of copper metabolism (CM) is closely associated with tumourigenesis and progression. Here, we investigated the impact of genes associated with copper metabolism (CMRGs) on the prognosis of OC, discovered various CM clusters, and built a risk model to evaluate patient prognosis, immunological features, and therapy response.Methods15 CMRGs affecting the prognosis of OC patients were identified in The Cancer Genome Atlas (TCGA). Consensus Clustering was used to identify two CM clusters. lasso-cox methods were used to establish the copper metabolism-related gene prognostic signature (CMRGPS) based on differentially expressed genes in the two clusters. The GSE63885 cohort was used as an external validation cohort. Expression of CM risk score-associated genes was verified by single-cell sequencing and quantitative real-time PCR (qRT-PCR). Nomograms were used to visually depict the clinical value of CMRGPS. Differences in clinical traits, immune cell infiltration, and tumor mutational load (TMB) between risk groups were also extensively examined. Tumour Immune Dysfunction and Rejection (TIDE) and Immune Phenotype Score (IPS) were used to validate whether CMRGPS could predict response to immunotherapy in OC patients.ResultsIn the TCGA and GSE63885 cohorts, we identified two CM clusters that differed significantly in terms of overall survival (OS) and tumor microenvironment. We then created a CMRGPS containing 11 genes to predict overall survival and confirmed its reliable predictive power for OC patients. The expression of CM risk score-related genes was validated by qRT-PCR. Patients with OC were divided into low-risk (LR) and high-risk (HR) groups based on the median CM risk score, with better survival in the LR group. The 5-year AUC value reached 0.74. Enrichment analysis showed that the LR group was associated with tumor immune-related pathways. The results of TIDE and IPS showed a better response to immunotherapy in the LR group.ConclusionOur study, therefore, provides a valuable tool to further guide clinical management and tailor the treatment of patients with OC, offering new insights into individualized treatment.
Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma
Authors: Nan Han , Guo Pengkun , Fan Jianing , Zeng Wen , Hu Chonghan , Zheng Can , Pan Bujian , Cao Yu , Ge Yiwen , Xue Xiangyang , Li Wenshu , Lin Kezhi 
Published: March 2023

BackgroundSuccinate dehydrogenase (SDH), one of the key enzymes in the tricarboxylic acid cycle, is mainly found in the mitochondria. SDH consists of four subunits encoding SDHA, SDHB, SDHC, and SDHD. The biological function of SDH is significantly related to cancer progression. Colorectal cancer (CRC) is one of the most common malignant tumors globally, whose most common histological subtype is colon adenocarcinoma (COAD). However, the correlation between SDH factors and COAD remains unclear.MethodsThe data on pan-cancer was obtained from The Cancer Genome Atlas (TCGA) database. Kaplan-Meier survival analysis showed the prognostic ability of SDHs. The cBioPortal database reflected genetic variations of SDHs. The correlation analysis was conducted between SDHs and mitochondrial energy metabolism genes (MMGs) and the protein-protein interaction (PPI) network was built. Consequently, Univariate and Multivariate Cox Regression Analysis on SDHs and other clinical characteristics were conducted. A nomogram was established. The ssGSEA analysis visualized the association between SDHs and immune infiltration. Immunophenoscore (IPS) explored the correlation between SDHs and immunotherapy, and the correlation between SDHs and targeted therapy was investigated through Genomics of Drug Sensitivity in Cancer. Finally, qPCR and immunohistochemistry detected SDHs’ expression.ResultsAfter assessing SDHs differential expression in pan-cancer, we found that SDHB, SDHC, and SDHD benefit COAD patients. The cBioPortal database demonstrated that SDHA was the top gene in mutation frequency rank. Correlation analysis mirrored a strong link between SDHs and MMGs. We formulated a nomogram and found that SDHB, SDHC, SDHD, and clinical characteristics correlated with COAD patients’ survival. For T helper cells, Th2 cells, and Tem, SDHA, SDHB, SDHC, and SDHD were significantly enriched in the high expression group. Moreover, COAD patients with high SDHA expression were more suitable for immunotherapy. And COAD patients with different SDHs’ expression have different sensitivity to targeted drugs. Further verifying the gene and protein expression levels of SDHs, we found that the tissues were consistent with the bioinformatics analysis.ConclusionsOur study analyzed the expression and prognostic value of SDHs in COAD, explored the pathway mechanisms involved, and the immune cell correlations, indicating that SDHs might be biomarkers for COAD patients.
Cross-reactive antibodies against Langat virus protect mice from lethal tick-borne encephalitis virus infection
Authors: Kubinski Mareike , Beicht Jana , Zdora Isabel , Saletti Giulietta , Kircher Magdalena , Petry-Gusmag Monique , Steffen Imke , Puff Christina , Jung Klaus , Baumgärtner Wolfgang , Rimmelzwaan Guus F. , Osterhaus Albert D. M. E. , Prajeeth Chittappen Kandiyil 
Published: March 2023

IntroductionNaturally attenuated Langat virus (LGTV) and highly pathogenic tick-borne encephalitis virus (TBEV) share antigenically similar viral proteins and are grouped together in the same flavivirus serocomplex. In the early 1970s, this has encouraged the usage of LGTV as a potential live attenuated vaccine against tick-borne encephalitis (TBE) until cases of encephalitis were reported among vaccinees. Previously, we have shown in a mouse model that immunity induced against LGTV protects mice against lethal TBEV challenge infection. However, the immune correlates of this protection have not been studied.MethodsWe used the strategy of adoptive transfer of either serum or T cells from LGTV infected mice into naïve recipient mice and challenged them with lethal dose of TBEV.ResultsWe show that mouse infection with LGTV induced both cross-reactive antibodies and T cells against TBEV. To identify correlates of protection, Monitoring the disease progression in these mice for 16 days post infection, showed that serum from LGTV infected mice efficiently protected from developing severe disease. On the other hand, adoptive transfer of T cells from LGTV infected mice failed to provide protection. Histopathological investigation of infected brains suggested a possible role of microglia and T cells in inflammatory processes within the brain.DiscussionOur data provide key information regarding the immune correlates of protection induced by LGTV infection of mice which may help design better vaccines against TBEV.
Human umbilical cord mesenchymal stem cell-derived exosomes promote murine skin wound healing by neutrophil and macrophage modulations revealed by single-cell RNA sequencing
Authors: Liu Yuanyuan , Zhang Mingwang , Liao Yong , Chen Hongbo , Su Dandan , Tao Yuandong , Li Jiangbo , Luo Kai , Wu Lihua , Zhang Xingyue , Yang Rongya 
Published: March 2023

IntroductionFull-thickness skin wound healing remains a serious undertaking for patients. While stem cell-derived exosomes have been proposed as a potential therapeutic approach, the underlying mechanism of action has yet to be fully elucidated. The current study aimed to investigate the impact of exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-Exosomes) on the single-cell transcriptome of neutrophils and macrophages in the context of wound healing.MethodsUtilizing single-cell RNA sequencing, the transcriptomic diversity of neutrophils and macrophages was analyzed in order to predict the cellular fate of these immune cells under the influence of hucMSC-Exosomes and to identify alterations of ligand-receptor interactions that may influence the wound microenvironment. The validity of the findings obtained from this analysis was subsequently corroborated by immunofluorescence, ELISA, and qRT-PCR. Neutrophil origins were characterized based on RNA velocity profiles.ResultsThe expression of RETNLG and SLC2A3 was associated with migrating neutrophils, while BCL2A1B was linked to proliferating neutrophils. The hucMSC-Exosomes group exhibited significantly higher levels of M1 macrophages (215 vs 76, p &lt; 0.00001), M2 macrophages (1231 vs 670, p &lt; 0.00001), and neutrophils (930 vs 157, p &lt; 0.00001) when compared to control group. Additionally, it was observed that hucMSC-Exosomes elicit alterations in the differentiation trajectories of macrophages towards more anti-inflammatory phenotypes, concomitant with changes in ligand-receptor interactions, thereby facilitating healing.DiscussionThis study has revealed the transcriptomic heterogeneity of neutrophils and macrophages in the context of skin wound repair following hucMSC-Exosomes interventions, providing a deeper understanding of cellular responses to hucMSC-Exosomes, a rising target of wound healing intervention.
Search, identification, and curation of cell and gene therapy product regulations using augmented intelligent systems
Authors: Schaut William , Shrivastav Akash , Ramakrishnan Srikanth , Bowden Robert 
Published: March 2023

BackgroundManually keeping up-to-date with regulations such as directives, guidance, laws, and ordinances related to cell and gene therapy is a labor-intensive process. We used machine learning (ML) algorithms to create an augmented intelligent system to optimize systematic screening of global regulations to improve efficiency and reduce overall labor and missed regulations.MethodsCombining Boolean logic and artificial intelligence (i.e., augmented intelligence) for the search process, ML algorithms were used to identify and suggest relevant cell and gene therapy regulations. Suggested regulations were delivered to a landing page for further subject matter expert (SME) tagging of words/phrases to provide system relevance on functional words. Ongoing learning from the repository regulations continued to increase system reliability and performance. The automated ability to train and retrain the system allows for continued refinement and improvement of system accuracy. Automated daily searches for applicable regulations in global databases provide ongoing opportunities to update the repository.ResultsCompared to manual searching, which required 3–4 SMEs to review ~115 regulations, the current system performance, with continuous system learning, requires 1 full-time equivalent to process approximately 9,000 regulations/day. Currently, system performance has 86% overall accuracy, a recommend recall of 87%, and a reject recall of 84%. A conservative search strategy is intentionally used to permit SMEs to assess low-recommended regulations in order to prevent missing any applicable regulations.ConclusionCompared to manual searches, our custom automated search system greatly improves the management of cell and gene therapy regulations and is efficient, cost effective, and accurate.
Phenotypic and genotypic characterization of Shiga toxin-producing Escherichia coli strains recovered from bovine carcasses in Uruguay
Authors: Mussio Paula , Martínez Inés , Luzardo Santiago , Navarro Armando , Leotta Gerardo , Varela Gustavo 
Published: March 2023

IntroductionShiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that cause food-borne diseases in humans. Cattle and derived foodstuffs play a known role as reservoir and vehicles, respectively. In Uruguay, information about the characteristics of circulating STEC in meat productive chain is scarce. The aim was to characterize STEC strains recovered from 800 bovine carcasses of different slaughterhouses.MethodsTo characterize STEC strains we use classical microbiological procedures, Whole Genome Sequencing (WGS) and FAO/WHO risk criteria.ResultsWe analyzed 39 STEC isolated from 20 establishments. They belonged to 21 different O-groups and 13 different H-types. Only one O157:H7 strain was characterized and the serotypes O130:H11(6), O174:H28(5), and O22:H8(5) prevailed. One strain showed resistance in vitro to tetracycline and genes for doxycycline, sulfonamide, streptomycin and fosfomycin resistance were detected. Thirty-three strains (84.6%) carried the subtypes Stx2a, Stx2c, or Stx2d. The gene eae was detected only in two strains (O157:H7, O182:H25). The most prevalent virulence genes found were lpfA (n = 38), ompA (n = 39), ompT (n = 39), iss (n = 38), and terC (n = 39). Within the set of STEC analyzed, the majority (81.5%) belonged to FAO/WHO’s risk classification levels 4 and 5 (lower risk). Besides, we detected STEC serotypes O22:H8, O113:H21, O130:H11, and O174:H21 belonged to level risk 2 associate with diarrhea, hemorrhagic colitis or Hemolytic-Uremic Syndrome (HUS). The only O157:H7 strain analyzed belonged to ST11. Thirty-eight isolates belonged to the Clermont type B1, while the O157:H7 was classified as E.DiscussionThe analyzed STEC showed high genomic diversity and harbor several genetic determinants associated with virulence, underlining the important role of WGS for a complete typing. In this set we did not detect non-O157 STEC previously isolated from local HUS cases. However, when interpreting this findings, the low number of isolates analyzed and some methodological limitations must be taken into account. Obtained data suggest that cattle constitute a local reservoir of non-O157 serotypes associated with severe diseases. Other studies are needed to assess the role of the local meat chain in the spread of STEC, especially those associated with severe diseases in humans.