bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Kombinasi Relaksasi Benson dan Slow Deep Breath Terhadap Intensitas Nyeri Post Operasi Sectio Caesarea di RSAB Harapan Kita Jakarta
Authors: Mahmudi Mahmudi , Dinaryanti Ratna Sari 
Published: June 2023

Postoperative pain is still one of the problems in nursing care, there are many therapies that can be done to reduce pain. One of the non-pharmacological actions is relaxation. Relaxation is a technique that relaxes the mind and body through a process that will progressively release muscle tension. The purpose of this study was to determine the effect of the combination of Benson's relaxation therapy and slow deep breath on the pain intensity of postoperative sectio caesarea patients. The research design used “ Quasy Experiment ” one group pre post test design without control. Samples were all post sectio caesaria pain patients in RSAB Harapan Kita Jakarta using purposive sampling method as many as 18 patients. Collecting data by interview and direct observation using the standard pain value Numeric Rate Scale. Analysis of the data using the Dependent T test . The results showed the mean value before the combination of Benson relaxation therapy and slow deep breath was 4.72 and the mean value after therapy was 3.00. So that the combination of Benson relaxation theraphy and slow deep breath can reduce the intensity of pain in patients post section caecaria with p value 0,0000. With this study, it is expected that the combination of Benson relaxation therapy and slow deep breath can be used as an non-pharmacology intervention to reduce pain in patients post operation sectio caecaria.
Peningkatan Nilai Dissolved Oksigen dan Penetralan pH pada Air Laut Menggunakan Destilasi Sederhana
Authors: Sangadjisowohy Idayani 
Published: June 2023

The issue of water pollution in big cities in Indonesia has shown very concerning signs. This study aims to determine the value of Dissolved Oxygen and pH before and after treatment using distillation. The method is simple experimental design with post test only group control design, carried out in the chemistry laboratory of the Ternate Polytechnic, with sample of 10 liters sea water. The researchers used simple distillation system consisting of electric stove, hose and container filled with cold water. Dissolved Oxygen in seawater samples before treatment did not meet the requirements for class 3 water which based on Government Regulation RI No 22 of 2021 must exceed 3 ppm while the test results showed that Dissolved Oxygen was only 1.7 ppm. dissolved oxygen in seawater samples with distillation treatment has met the requirements for class 3 water which must exceed 3 ppm while the test results show that DO is 6.7 ppm. pH in seawater is still in the category that meets the requirements. pH in seawater after distillation has changed from alkaline to neutral with a test number of 7. In conclusion, distillation is able to increase Dissolved Oxygen and neutralize pH. The recommendation for further research to carry out sampling at several points in order to obtain broader water characteristics, so that it can be used as a reference for decision making at the local government.
Genetic risk factors for postoperative atrial fibrillation—a nationwide genome-wide association study (GWAS)
Authors: Christensen Mathias A. , Bonde Alexander , Sillesen Martin 
Published: June 2023

BackgroundAtrial fibrillation (AF) is a major cause of morbidity with a high prevalence among the elderly and has an established genetic disposition. Surgery is a well-known risk factor for AF; however, it is currently not recognized how much common genetic variants influence the postoperative risk. The purpose of this study was to identify Single Nucleotide Polymorphisms associated with postoperative AF.MethodsThe UK Biobank was utilized to conduct a Genome-Wide Association Study (GWAS) to identify variants associated with AF after surgery. An initial discovery GWAS was performed in patients that had undergone surgery with subsequent replication in a unique non-surgical cohort. In the surgical cohort, cases were defined as newly diagnosed AF within 30 days after surgery. The threshold for significance was set at 5 × 10−8.ResultsAfter quality control, 144,196 surgical patients with 254,068 SNPs were left for analysis. Two variants (rs17042171 (p = 4.86 × 10−15) and rs17042081 (p = 7.12 × 10−15)) near the PITX2-gene reached statistical significance. These variants were replicated in the non-surgical cohort (1.39 × 10−101 and 1.27 × 10−93, respectively). Several other loci were significantly associated with AF in the non-surgical cohort.ConclusionIn this GWAS-analysis of a large national biobank, we identified 2 variants that were significantly associated with postoperative AF. These variants were subsequently replicated in a unique non-surgical cohort. These findings bring new insight in the genetics of postoperative AF and may help identify at-risk patients and guide management.
Mechanisms by which statins protect endothelial cells from radiation-induced injury in the carotid artery
Authors: Ait-Aissa Karima , Leng Linette N. , Lindsey Nathanial R. , Guo Xutong , Juhr Denise , Koval Olha M. , Grumbach Isabella M. 
Published: June 2023

BackgroundThe incidental use of statins during radiation therapy has been associated with a reduced long-term risk of developing atherosclerotic cardiovascular disease. However, the mechanisms by which statins protect the vasculature from irradiation injury remain poorly understood.ObjectivesIdentify the mechanisms by which the hydrophilic and lipophilic statins pravastatin and atorvastatin preserve endothelial function after irradiation.MethodsCultured human coronary and umbilical vein endothelial cells irradiated with 4 Gy and mice subjected to 12 Gy head-and-neck irradiation were pretreated with statins and tested for endothelial dysfunction, nitric oxide production, oxidative stress, and various mitochondrial phenotypes at 24 and 240 h after irradiation.ResultsBoth pravastatin (hydrophilic) and atorvastatin (lipophilic) were sufficient to prevent the loss of endothelium-dependent relaxation of arteries after head-and-neck irradiation, preserve the production of nitric oxide by endothelial cells, and suppress the cytosolic reactive oxidative stress associated with irradiation. However, only pravastatin inhibited irradiation-induced production of mitochondrial superoxide; damage to the mitochondrial DNA; loss of electron transport chain activity; and expression of inflammatory markers.ConclusionsOur findings reveal some mechanistic underpinnings of the vasoprotective effects of statins after irradiation. Whereas both pravastatin and atorvastatin can shield from endothelial dysfunction after irradiation, pravastatin additionally suppresses mitochondrial injury and inflammatory responses involving mitochondria. Clinical follow-up studies will be necessary to determine whether hydrophilic statins are more effective than their lipophilic counterparts in reducing the risk of cardiovascular disease in patients undergoing radiation therapy.
Clustering features and seismogenesis of the 2014 M6.6 Jinggu earthquake in Yunnan Province, China
Authors: Zheng Jianchang 
Published: June 2023

Seismic activities can be seen as the composition of background and clustering earthquakes. It is important to identify seismicity clusters from background events. Based on the Nearest Neighbour Distance algorithm proposed by Zaliapin, we use the Gaussian mixture model (GMM) to fit its spatiotemporal distribution and use the probability corresponding to clustering seismicity in the GMM model as the clustering ratio. After testing with synthetic catalogues under the ETAS (epidemic-type aftershock sequence) model, We believe the method can discriminate cluster events from randomly occurring background seismicity in a more physical background. We investigate the seismicity and its clustering features before the M6.6 Jinggu earthquake in Yunnan Province, China on 7 October 2014. Our results show the following: 1) The seismogenic process of this strong earthquake has three stages, which are already described by the IPE model (the model is similiar to dilatancy diffusion model, growth of cracks is also involved but diffusion of water in and out of the focal region is not required); 2) The main shock might have been caused by the breaking of a local locked barrier in the hypocentre, and the meta-instability stage was sustained for about 1 year on the fault. From this study, we conclude that the evolution of seismicity clustering features can reflect changes in stress in the crust, and it is closely connected to the seismogenic process of a strong earthquake.
Machine learning-assisted analysis of epithelial mesenchymal transition pathway for prognostic stratification and immune infiltration assessment in ovarian cancer
Authors: Li Qian , Xiao Xiyun , Feng Jing , Yan Ruixue , Xi Jie 
Published: June 2023

BackgroundOvarian cancer is the most lethal gynaecological malignancy, and serous ovarian cancer (SOC) is one of the more important pathological subtypes. Previous studies have reported a significant association of epithelial tomesenchymal transition (EMT) with invasive metastasis and immune modulation of SOC, however, there is a lack of prognostic and immune infiltration biomarkers reported for SOC based on EMT.MethodsGene expression data for ovarian cancer and corresponding patient clinical data were collected from the TCGA database and the GEO database, and cell type annotation and spatial expression analysis were performed on single cell sequencing data from the GEO database. To understand the cell type distribution of EMT-related genes in SOC single-cell data and the enrichment relationships of biological pathways and tumour functions. In addition, GO functional annotation analysis and KEGG pathway enrichment analysis were performed on mRNAs predominantly expressed with EMT to predict the biological function of EMT in ovarian cancer. The major differential genes of EMT were screened to construct a prognostic risk prediction model for SOC patients. Data from 173 SOC patient samples obtained from the GSE53963 database were used to validate the prognostic risk prediction model for ovarian cancer. Here we also analysed the direct association between SOC immune infiltration and immune cell modulation and EMT risk score. and calculate drug sensitivity scores in the GDSC database.In addition, we assessed the specific relationship between GAS1 gene and SOC cell lines.ResultsSingle cell transcriptome analysis in the GEO database annotated the major cell types of SOC samples, including: T cell, Myeloid, Epithelial cell, Fibroblast, Endothelial cell, and Bcell. cellchat revealed several cell type interactions that were shown to be associated with EMT-mediated SOC invasion and metastasis. A prognostic stratification model for SOC was constructed based on EMT-related differential genes, and the Kapan-Meier test showed that this biomarker had significant prognostic stratification value for several independent SOC databases. The EMT risk score has good stratification and identification properties for drug sensitivity in the GDSC database.ConclusionsThis study constructed a prognostic stratification biomarker based on EMT-related risk genes for immune infiltration mechanisms and drug sensitivity analysis studies in SOC. This lays the foundation for in-depth clinical studies on the role of EMT in immune regulation and related pathway alterations in SOC. It is also hoped to provide effective potential solutions for early diagnosis and clinical treatment of ovarian cancer.
Restless leg syndrome in multiple sclerosis: a case–control study
Authors: Aljarallah Salman , Alkhawajah Nuha , Aldosari Omar , Alhuqbani Mohammed , Alqifari Faisal , Alkhuwaitir Bassam , Aldawood Abdullah , Alshenawy Omar , BaHammam Ahmed S. 
Published: June 2023

ObjectivesThis study assessed the prevalence of restless leg syndrome (RLS) among patients with multiple sclerosis (pwMS) and the association between RLS and MS disease duration, sleep disturbance, and daytime fatigue.MethodsIn this cross-sectional study, we interviewed 123 patients via phone calls using preset questionnaires containing the International Restless Legs Syndrome Study Group (IRLSSG) diagnostic criteria, Pittsburgh Sleep Quality Index (PSQI), and Fatigue Severity Scale (FSS) diagnostic criteria validated in both Arabic and English. The prevalence of RLS in MS was compared to a group of healthy controls.ResultsThe prevalence of RLS in pwMS, defined by meeting all four requirements included in the IRLSSG diagnostic criteria, was 30.3% compared to 8.3% in the control group. About 27.3% had mild RLS, 36.4% presented with moderate, and the remaining had severe or very severe symptoms. Patients with MS who experience RLS had a 2.8 times higher risk of fatigue compared to pwMS without RLS. pwMS with RLS had worse sleep quality, with a mean difference of 0.64 in the global PSQI score. Sleep disturbance and latency had the most significant impact on sleep quality.ConclusionThe prevalence of RLS among MS patients was significantly higher compared to the control group. We recommend educating neurologists and general physicians to increase their awareness of the increasing prevalence of RLS and its association with fatigue and sleep disturbance in patients with MS.
Increased neurocardiological interplay after mindfulness meditation: a brain oscillation-based approach
Authors: Gao Junling , Sun Rui , Leung Hang Kin , Roberts Adam , Wu Bonnie Wai Yan , Tsang Eric W. , Tang Andrew C. W. , Sik Hin Hung 
Published: June 2023

BackgroundBrain oscillations facilitate interaction within the brain network and between the brain and heart activities, and the alpha wave, as a prominent brain oscillation, plays a major role in these coherent activities. We hypothesize that mindfully breathing can make the brain and heart activities more coherent in terms of increased connectivity between the electroencephalogram (EEG) and electrocardiogram (ECG) signals.MethodsEleven participants (28–52 years) attended 8 weeks of Mindfulness Based Stress Reduction (MBSR) training. EEG and ECG data of two states of mindful breathing and rest, both eye-closed, were recorded before and after the training. EEGLAB was used to analyze the alpha band (8–12 Hz) power, alpha peak frequency (APF), peak power and coherence. FMRIB toolbox was used to extract the ECG data. Heart coherence (HC) and heartbeat evoked potential (HEP) were calculated for further correlation analysis.ResultsAfter 8 weeks of MBSR training, the correlation between APF and HC increased significantly in the middle frontal region and bilateral temporal regions. The correlation between alpha coherence and heart coherence had similar changes, while alpha peak power did not reflect such changes. In contrast, spectrum analysis alone did not show difference before and after MBSR training.ConclusionThe brain works in rhythmic oscillation, and this rhythmic connection becomes more coherent with cardiac activity after 8 weeks of MBSR training. Individual APF is relatively stable and its interplay with cardiac activity may be a more sensitive index than power spectrum by monitoring the brain-heart connection. This preliminary study has important implications for the neuroscientific measurement of meditative practice.
Botulinum toxin A decreases neural activity in pain-related brain regions in individuals with chronic ocular pain and photophobia
Authors: Reyes Nicholas , Huang Jaxon J. , Choudhury Anjalee , Pondelis Nicholas , Locatelli Elyana V. , Felix Elizabeth R. , Pattany Pradip M. , Galor Anat , Moulton Eric A. 
Published: June 2023

IntroductionTo examine the effect of botulinum toxin A (BoNT-A) on neural mechanisms underlying pain and photophobia using functional magnetic resonance imaging (fMRI) in individuals with chronic ocular pain.MethodsTwelve subjects with chronic ocular pain and light sensitivity were recruited from the Miami Veterans Affairs eye clinic. Inclusion criteria were: (1) chronic ocular pain; (2) presence of ocular pain over 1 week recall; and (3) presence of photophobia. All individuals underwent an ocular surface examination to capture tear parameters before and 4–6 weeks after BoNT-A injections. Using an event-related fMRI design, subjects were presented with light stimuli during two fMRI scans, once before and 4–6 weeks after BoNT-A injection. Light evoked unpleasantness ratings were reported by subjects after each scan. Whole brain blood oxygen level dependent (BOLD) responses to light stimuli were analyzed.ResultsAt baseline, all subjects reported unpleasantness with light stimulation (average: 70.8 ± 32.0). Four to six weeks after BoNT-A injection, unpleasantness scores decreased (48.1 ± 33.6), but the change was not significant. On an individual level, 50% of subjects had decreased unpleasantness ratings in response to light stimulation compared to baseline (“responders,” n = 6), while 50% had equivalent (n = 3) or increased (n = 3) unpleasantness (“non-responders”). At baseline, several differences were noted between responders and non-responders; responders had higher baseline unpleasantness ratings to light, higher symptoms of depression, and more frequent use of antidepressants and anxiolytics, compared to non-responders. Group analysis at baseline displayed light-evoked BOLD responses in bilateral primary somatosensory (S1), bilateral secondary somatosensory (S2), bilateral anterior insula, paracingulate gyrus, midcingulate cortex (MCC), bilateral frontal pole, bilateral cerebellar hemispheric lobule VI, vermis, bilateral cerebellar crus I and II, and visual cortices. BoNT-A injections significantly decreased light evoked BOLD responses in bilateral S1, S2 cortices, cerebellar hemispheric lobule VI, cerebellar crus I, and left cerebellar crus II. BoNT-A responders displayed activation of the spinal trigeminal nucleus at baseline where non-responders did not.DiscussionBoNT-A injections modulate light-evoked activation of pain-related brain systems and photophobia symptoms in some individuals with chronic ocular pain. These effects are associated with decreased activation in areas responsible for processing the sensory-discriminative, affective, dimensions, and motor responses to pain.
Sex-specific responses in glucose-insulin homeostasis and lipoprotein-lipid components after high-dose supplementation with marine n-3 PUFAs in abdominal obesity: a randomized double-blind crossover study
Authors: Laupsa-Borge Johnny , Grytten Elise , Bohov Pavol , Bjørndal Bodil , Strand Elin , Skorve Jon , Nordrehaug Jan Erik , Berge Rolf K. , Rostrup Espen , Mellgren Gunnar , Dankel Simon N. , Nygård Ottar K. 
Published: June 2023

BackgroundClinical studies on effects of marine-derived omega-3 (n-3) polyunsaturated fatty acids (PUFAs), mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and the plant-derived omega-6 (n-6) PUFA linoleic acid (LA) on lipoprotein-lipid components and glucose-insulin homeostasis have shown conflicting results, which may partly be explained by differential responses in females and males. However, we have lacked data on sexual dimorphism in the response of cardiometabolic risk markers following increased consumption of n-3 or n-6 PUFAs.ObjectiveTo explore sex-specific responses after n-3 (EPA + DHA) or n-6 (LA) PUFA supplementation on circulating lipoprotein subfractions, standard lipids, apolipoproteins, fatty acids in red blood cell membranes, and markers of glycemic control/insulin sensitivity among people with abdominal obesity.MethodsThis was a randomized double-blind crossover study with two 7-week intervention periods separated by a 9-week washout phase. Females (n = 16) were supplemented with 3 g/d of EPA + DHA (fish oil) or 15 g/d of LA (safflower oil), while males (n = 23) received a dose of 4 g/d of EPA + DHA or 20 g/d of LA. In fasting blood samples, we measured lipoprotein particle subclasses, standard lipids, apolipoproteins, fatty acid profiles, and markers of glycemic control/insulin sensitivity.ResultsThe between-sex difference in relative change scores was significant after n-3 for total high-density lipoproteins (females/males: −11%*/−3.3%, p = 0.036; *: significant within-sex change), high-density lipoprotein particle size (+2.1%*/−0.1%, p = 0.045), and arachidonic acid (−8.3%*/−12%*, p = 0.012), and after n-6 for total (+37%*/+2.1%, p = 0.041) and small very-low-density lipoproteins (+97%*/+14%, p = 0.021), and lipoprotein (a) (−16%*/+0.1%, p = 0.028). Circulating markers of glucose-insulin homeostasis differed significantly after n-3 for glucose (females/males: −2.1%/+3.9%*, p = 0.029), insulin (−31%*/+16%, p &lt; 0.001), insulin C-peptide (−12%*/+13%*, p = 0.001), homeostasis model assessment of insulin resistance index 2 (−12%*/+14%*, p = 0.001) and insulin sensitivity index 2 (+14%*/−12%*, p = 0.001), and quantitative insulin sensitivity check index (+4.9%*/−3.4%*, p &lt; 0.001).ConclusionWe found sex-specific responses after high-dose n-3 (but not n-6) supplementation in circulating markers of glycemic control/insulin sensitivity, which improved in females but worsened in males. This may partly be related to the sex differences we observed in several components of the lipoprotein-lipid profile following the n-3 intervention.Clinical trial registrationhttps://clinicaltrials.gov/, identifier [NCT02647333].
Deep eutectic solvent-ultrasound assisted extraction as a green approach for enhanced extraction of naringenin from Searsia tripartita and retained their bioactivities
Authors: El Maaiden Ezzouhra , El Kahia Houda , Nasser Boubker , Moustaid Khadija , Qarah Nagib , Boukcim Hassan , Hirich Abdelaziz , Kouisni Lamfeddal , El Kharrassi Youssef 
Published: June 2023

BackgroundNaringenin (NA) is a natural flavonoid used in the formulation of a wide range of pharmaceutical, fragrance, and cosmetic products. In this research, NA was extracted from Searsia tripartita using an environmentally friendly, high efficiency extraction method: an ultrasound-assisted extraction with deep eutectic solvents (UAE-DES).MethodsSix natural deep eutectic solvent systems were tested. Choline chloride was used as the hydrogen bond acceptor (HBA), and formic acid, ethylene glycol, lactic acid, urea, glycerol, and citric acid were used as hydrogen bond donors (HBD).ResultsBased on the results of single-factor experiments, response surface methodology using a Box-Behnken design was applied to determine the optimal conditions for UAE-DES. According to the results, the optimal NA extraction parameters were as follows: DES-1 consisted of choline chloride (HBA) and formic acid (HBD) in a mole ratio of 2:1, an extraction time of 10 min, an extraction temperature of 50°C, an ultrasonic amplitude of 75 W, and a solid-liquid ratio of 1/60 g/mL. Extracted NA was shown to inhibit the activity of different enzymes in vitro, including α-amylase, acetylcholinesterase, butyrylcholinesterase, tyrosinase, elastase, collagenase, and hyaluronidase.ConclusionThus, the UAE-DES technique produced high-efficiency NA extraction while retaining bioactivity, implying broad application potential, and making it worthy of consideration as a high-throughput green extraction method.