bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glasdegib, A Hedgehog Signaling Inhibitor Quantification by Development of Validated LC-ESI-MS/MS (QTRAP) Method in Human Plasma
Authors: rao Yamarthi Venkateswara , Chimakurthy Jithendra 
Published: May 2023

Abstract
Cancer medication Glasdegib, created by Pfizer, has FDA approval. It serves as a tiny molecule an inhibitor of sonic hedgehog, a protein that is overexpressed in several cancer types. Like the majority of medications in its class, it inhibits the smoothened form that contains the hedgehog's sonic receptor (SMO). Clinical trials in phase II are ongoing in four instances. One is testing glasdegib's ability to treat myelofibrosis in patients whose condition was resistant to ruxolitinib treatment. We're aware of no method for precisely measuring Glasdegib, an anti-cancer medication, in biological fluids at the moment. A novel, simple& robust LC-MS/MS method was developed in to detect Glasdegib in a biological fluid. Here, we came up with a novel bioanalytical method using isocratic elution with a symmetric C18 column (150 x 4.6 mm, 3.5 µm), acn: 0.1% formic acid (30:70) was used as mobile phase pH of the mobile phase was adjusted to 4.0 using 0.1% formic acid at rate of flow for 1 ml/min. The drug retention was found as 2.622 minutes, and the total analysis time was set for 6 minutes. The Glasdegib calibration curve was drawn at concentrations ranging from 6 ng to 120 ng/ml of Glasdegib, with regression coefficient (r2) of 0.999. System suitability parameters for resolution, tailing factor and theoretical plates, are found in acceptable limits. The recovery studies indicated that 99.94% of the drug can be extracted using the developed method in an acceptable ratio. The results of the matrix effect study show that the matrix has no impact on recovery; the result is 98.55%, and some other validation parameters are accuracy, linearity, robustness, LOD and LOQ are found in acceptable limits. The developed method should follow an analytical approach like precise, sensitive, and accurate for the evaluation of Glasdegib in biological matrix. The results of the accelerated stability studies reveal that the drug is stable under various conditions, according to USFDA Guidelines.
Myocardial protection strategy: Reducing cardiac arrest time to improve myocardial protection in patients undergoing mitral valve replacement accompanying coronary artery bypass grafting
Authors: Yan Wenlong , Han Bo , Wang Yangyang , Zheng Xin , Wu Yuhui , Hu Haoyu , Yang Sumin 
Published: May 2023

Abstract
Background: Compared with isolated mitral valve replacement (MVR) or isolated coronary artery bypass grafting (CABG), MVR + CABG surgery is associated with higher mortality and complication rate. This study investigated the safety and efficacy of the myocardial protection technique to short cardioplegic arrest time in patients who underwent MVR + CABG.
Methods:This study enrolled patients diagnosed with mitral valve disease and coronary artery disease who underwent MVR + CABG between January 2014 and January 2022. The patients were divided into the CABG without cardioplegic arrest (CA) group (novel group) and CABG with CA group (conventional group) according to surgical methods. The baseline data of the two groups of patients were corrected by the propensity score matching (PSM) method. Endpoints assessed were operative mortality (OM), major morbidity (MM)and 5-year follow-up clinical outcomes.
Results: 810 patients (256 in the novel group and 554 in the conventional group) were enrolled in this study. PSM resulted in a total of 460 patients (230 pairs). There was no significant difference in OM between the two groups (6.5% vs 10.8%, P= 0.098). The MM was significantly lower in the novel group than the conventional group (24.3% vs 33.9%, P= 0.024). Mean operation time (285 ± 55 vs 298 ± 60 minutes, P= 0.016), cardiopulmonary bypass (CPB) time (115 ± 33 vs 139 ± 38 minutes, P < 0.001), CA time (66 ± 11 vs 85 ± 16 minutes, P < 0.001), intensive care unit stay time (33.6 ± 12.3 vs 36.6 ± 14.5 hours, P = 0.017) and post-operative length of stay (11.1 ± 3.6 vs 11.9 ± 4.1 days, P = 0.027) were significantly shorter in the novel group than in the conventional group. There was no significant difference between the novel group and the conventional group in terms of death from any cause, valve-related complications, myocardial infarction and any repeat revascularisation in 5 years follow-up.
Conclusions: The novel group is associated with excellent short-term outcomes and comparable medium-term outcomes for patients undergoing MVR + CABG. Myocardial protection strategy to short cardioplegic arrest time in patients undergoing MVR + CABG was surgically feasible, reproducible, and safe.
Determination of electrical facies using MRGC and ANN clustering methods
Authors: Eftekhari Seyedeh Hajar , Memariani Mahmoud , Maleki Zahra , Aleali Mohsen , Kianoush Pooria 
Published: May 2023

Abstract
The present research was performed on Iran's most crucial reservoir formation (Asmari Formation) in the Mansouri oil field in southwest Iran. This Formation is generally composed of carbonate lithologic, but it has sandstone layers in some zones. It is also the youngest reservoir rock in Iran. In the first step, the lithology of the Asmari Formation was determined by cross-lithology diagrams, and a combination of lime, dolomite, anhydrite, and sandstone with shaley layers was estimated. Also, in the sequence of this geological/reservoir zoning formation, five zones were identified with different geological/reservoir conditions, and the average of each of these petrophysical parameters was calculated in each zone, with the best reservoir quality in zones 3 and 5 with sandstone/shale lithology. Also, this study determines facies clustering methods MRGC and ANN and reading of gamma, neutron, density, acoustic and resistivity diagrams, facies having common geological/reservoir conditions in different subdivisions. For this purpose, the combined log readings of DT, RHOB, GR, Sw, NPHI, and PHIE were used. After applying the software (Geolog) method, an optimal model with 8 clusters (facies) was obtained. The reservoir features and plots in the software for better separation of rock species studied. Finally, five facies with different lithological compositions and reservoir conditions were identified in this study. The information obtained from determining electrical facies by clustering method determines the reservoir zone from non-reservoir and qualitatively (good, medium, and poor) of Mansouri oilfield were achieved.
Association between specialist pediatric cardiac transfer and prognosis of neonates with critical congenital heart disease: A cohort study
Authors: Li Chunxiang , Gong Xiaolei , Xu Zhuoming , Liu Jinlong , Zhang Haibo , Zhu Limin 
Published: May 2023

Abstract
Objective: To explore whether specialist pediatric cardiac transfer could improve the prognosis of neonates with critical congenital heart disease (CCHD).
Methods: This cohort study retrospectively collected the clinical data of neonates diagnosed with CCHD who underwent treatment at the cardiac intensive care unit of Shanghai Children’s Medical Center between January 2018 and December 2021. The neonates were classified into the specialist pediatric cardiac transfer (SPCT) and non-SPCT groups. Propensity score matching (PSM) was used to match the two groups. The surgical outcome was the postoperative survival of the neonates.
Results: During the study period, 357 neonates with CCHD were treated and included, of which 16 died before surgery, all in the non-SPCT group. After PSM, compared with the non-SPCT group, the SPCT group showed a lower rate of inotropic drug use (57.3% vs. 77.5%, P=0.004), a lower rate of unplanned emergent operation (29.2% vs. 53.9%, p=0.001), a higher total survival rate (92.1% vs. 82.0%, p =0.044), and a lower preoperative mortality rate (0% vs. 4.5%, p =0.043). The multivariable analysis showed that body weight at surgery (HR=0.444, 95%CI: 0.273-0.711, p=0.001) and unplanned emergent surgery (HR=5.227, 95%CI: 2.521-10.834, p<0.001) were independently associated with mortality in neonates with CCHD.
Conclusion:Low body weight and unplanned emergency surgery are independent risk factors for the death of neonates with CCHD. Although SPCT is unrelated to the surgery mortality rate of newborns with CCHD, it can reduce the incidence of unplanned emergency surgery and increase the total survival rate.
Comparison of the relative expression of the IGF gene family in the bone marrow of patients with AML compared with normal individuals
Authors: Moonesi Mohammadreza , Omran Hanie Mehdizade , Khosravi Saeed Zaka , Ramshe Samira Molaei , Farsani Mehdi allahbakhshian , Solali Saeed , Hagh Majid Farshdousti 
Published: May 2023

Abstract
Introduction:
One of the most common types of leukemia is acute myeloid leukemia (AML). Intrinsic and extrinsic factors may lead to AML. Insulin-like growth factor (IGF) is a mitogenic intermediate from the liver that regulates growth and proliferation in response to GH. In this study, we examined the expression of IGF family genes in AML patients (M3 and Non-M3) and compared them with normal samples.
Methods
Forty bone marrow samples from recently diagnosed AML patients along with 15 samples from subjects without hematological malignancies were collected. For molecular tests, RNA extraction and cDNA synthesis were performed. Finally, IGF1, IGF2, IGFBP3, IGF1R, and IGF2R gene expression were examined by Real-Time PCR.
Results
IGF1, IGF1R, and IGFBP3 gene expression were significantly increased in patients with AML. In contrast, IGF2 and IGF2R genes did not show significant expression changes between the two groups.
Conclusion
The expression in this gene family soared in AML patients' bone marrow, compared to normal subjects. This can be caused by malignant cells in the bone marrow. These malignant cells express proteins that increase the number of malignant cells. Moreover, they can be considered as diagnostic biomarkers or therapeutic targets with further research.
Characterization and synthesis of mesoporous silica nanoparticles modified using Zn, NH2, and graphene oxide containing Doxorubicin and assessment of its apoptosis induction, cytotoxicity, and anti-metastatic effects on MDA-MB-231 cell line
Authors: Akhtari Niyayesh , Tafizi Farzaneh , Naseh Vahid 
Published: May 2023

Abstract
In the current study, Mesoporous silica nanoparticles (MSNs) were provided and functionalized using zinc, amine, and graphene oxide (GO) (MZNG). Then, they were applied to deliver Doxorubicin, an anticancer drug, to breast cancer cells. The characterization findings indicated that MZNG loaded with DOX had a smooth surface and a spherical shape without homogeneous distribution with a particle size of around 215 nm. The high entrapment efficiency of DOX was observed for MZNGs at pH 7.4. Cytotoxicity results indicated that free DOX had high compatibility with HFF cells compared to DOX loaded into MZNG formulations, while DOX-loaded nanoparticles significantly increased the cytotoxicity against MDA-MB-231compared to free drugs and non-loaded nanoparticles. Moreover, DOX-loaded nanoparticles displayed increased apoptotic potential in MDA-MB-231 compared to free DOX and non-loaded nanoparticles (MZNGs). Upon treatment with samples, a downregulation of MMP-9 and Bcl-2 genes and an upregulation of Bax, Caspase 3, and Mir-193 genes were found. The prepared Nano-formulation holds great promise for treating breast cancer.
Magnetic properties of roadside dust: A case study in Baghdad City, Iraq
Authors: Ameen Nawrass , Khwedim Kareem 
Published: May 2023

Abstract
Magnetic measurements were carried out on roadside dust from the main urban area of Baghdad City, Iraq. The area is heavy traffic as the highway passes through the area from the southern provinces and the roadside represents an effective trap for dust. Concentration-related magnetic parameters, such as magnetic susceptibility (χ), anhysteretic remanent magnetization (ARM), saturation isothermal remanent magnetization (SIRM), and S-ratio of samples had been measured for four areas (A, B, C, and D). Results showed that a magnetite-like phase prevails the magnetic phases of the street dust. Concentration-dependent magnetic parameters of samples increased on the main roads. Mass-specific magnetic susceptibility (ꭕ) is strongly correlated with the susceptibility of Anhysteritic remanent magnetization (χARM) where the correlation coefficients are 0.7 and 0.9, respectively, indicating that the major participant is ferro(i)magnetic minerals (magnetite). S-ratio supports our hypothesis as the mean values were 0.96, 0.95, 0.97, and 0.96 for the four areas (A, B, C, and D), respectively. The primary anthropogenic sources of street dust magnetic particles are vehicle emissions, tire abrasion, and building materials. These findings enable the use of magnetic techniques as straightforward, quick, and non-destructive tools for determining the levels of heavy metal contamination during the process of urbanization.
Microbiome analysis of chronic suppurative otitis media and middle-ear cholesteatoma in China
Authors: Liang Qiulin , Long Ruiqing , Li Shuling , Jiang Chaowu , Gao Jingyu , Cheng Sheng , Liu Zhuohui , Ruan Biao 
Published: May 2023

Abstract
Here, the bacterial diversity in chronic suppurative otitis media (CSOM) and middle-ear cholesteatoma (MEC) was analysed. Nine and twenty-nine patients with CSOM and MEC, respectively, were recruited. Middle-ear lesion tissue was collected intraoperatively after opening the tympanic sinus and mastoid cavity under general anaesthesia and sterile conditions. The full-length 16S rRNA genome sequenced using third-generation sequencing (TGS) was then used to profile the bacterial community of each patient. Principal coordinate analysis showed that PC1 and PC2 could explain more than 50% of the between-group differences. Similarity analysis using the binary Jaccard distance matrix indicated that between-group differences were greater than within-group differences (P < 0.05). Staphylococcus aureus was the most common strain in both groups. At the species level, the abundance of Anaerococcus_octavius was significantly different between both groups (P < 0.05). According to the linear discriminant effect size analysis, at the class and genus levels, Alphaproteobacteria and Bacillus were abundant in the CSOM group, respectively. Peptoniphilus_grossensis and Peptostreptococcaceae_bacterium_oral_taxon_929 were abundant at the species level in the MEC group (P < 0.05). Four COG (Clusters of Orthologous Groups ) functions at level 2 were significantly different between the two groups (P < 0.05). The CSOM and MEC groups were inhabited by more diverse microbial communities. This could guide the regular use of antibiotics and decrease the likelihood of multidrug-resistant bacteria formation. Further research on the pathogenic diseases of CSOM and MEC will focus on the functional differences between flora.
In-Silico Drug Designing of Anti-Polymerizing Activity of Caffeic acid derivatives for the Treatment Of Sickle Cell Disease
Authors: Verma Deepshikha , namdeo KP 
Published: May 2023

Abstract
A series of novel caffeic acid derivatives that were designed utilizing autodock vina software are being developed as a potent anti-sickling agent in an effort to produce anti-polymerizing activity that will lessen complications in sickle cell disease. When different chemical groups are substituted on the hydroxyl benzaldehyde ring at the meta-position to create caffeic acid analogues, docking studies reveal strong binding affinity to the protein's active region. The results of this study may lead to the development of more effective anti-sickling therapies. Caffeic acid, in its many forms, is one of the most promising secondary metabolites for the treatment of a wide range of illnesses and diseases. The toxicity & biological activity of 8 chemicals are analyzed in this study using ADMET and Auto dock vina software for calculating binding score. Out of 8 compounds from the Caffeic acid derivatives substitution at meta position can be regarded as the safest showing best binding affinity can be further synthesized to get new drug.
Impact of Microfinance Credit Access on Household Income and Welfare: Evidence from Ethiopia
Authors: Wassie Asmamaw Getnet 
Published: May 2023

Abstract
This study examined the impact of microfinance credit access on households’ income and welfare case study in rural South Gondar zone, Ethiopia. The main objective of the study was investigating the impact of microfinance credit access on households’ income and welfare. The study used cross sectional data. The study surveyed 422 households. Half of the surveyed households were credit takers but not the remaining. The treatment variable (D) of the study is credit access. The outcome variables of the study are households’ income and welfare. The study employed both descriptive and econometric model to achieve the objective of the study. The propensity score matching (PSM) was employed in the econometric part of the study to estimate the impact of the credit on outcome variables of the study. Among matching methods, kernel (0.1) was employed since it satisfies the criterion of best matching between treatment unit and controlled unit. The study found that average treatment effect (ATT) on the treated group in terms of income is less than its counterpart. However, average treatment effect (ATT) on treated group in terms of welfare is greater than its counterpart.
Investigation of double layer corrugated steel plate shear walls in multi-story frames
Authors: Kiani Mahdi , Enayati Hamed 
Published: May 2023

Abstract
The flat steel plate shear walls (FPSW) are widely used as a part of lateral force resisting systems, however easily buckle under lateral cyclic loads. As an alternative are corrugated steel plate shear walls (CPSW) in which the infill flat steel plate is replaced with a corrugated steel plate. CPSW has some advantages over FPSW such as in-plane and out of plane stiffness, strength and ductility. Despite these advantages, the applicable thickness of corrugated plates due to the instrumental capability is limited thus, a double layer corrugated steel plate shear wall (DCPSW) composed of two similar corrugated steel plates was introduced. As previous studies in this context more focused on single-story frames and there is not a rich literature on DCPSW systems, in this paper, 48 two-dimensional multi-story frames were numerically studied under monotonic pushover lateral excitations. According to results, corrugated shear walls have less lateral strength comparing to FPSW and with increasing story numbers the difference are smaller. The corrugated walls are more ductile, especially in the case of shorter frames. In addition, Frames with DCPSWs are associated with the highest inelastic deformations which can be considered as an advantage over CPSWs.
Valorization of sweet potato (Ipomoea batatas) leaves by producing a novel herbal tea and its fermentation with kombucha consortium
Authors: Weber Caroline Trevisan , Angnes Fabiane , Carvalho Débora Gonçalves , Trierweiler Luciane Ferreira , Trierweiler Jorge Otávio 
Published: May 2023

Abstract
Tea and kombucha consumption has increased in recent years. Despite the big Brazilian sweet potato production, the leaves are generally discarded. This work aims to evaluate the potential of using this residual raw material to produce tea and kombucha and analyze its phenolic profile. Sweet potato leaves' tea presented a total phenolic content higher than the commercial herbal teas evaluated and an antioxidant capacity similar to chamomile and fennel teas. For kombucha, a blend of up to 75% of sweet potato leaves' tea can be used and still match the phenolic content of the commercial kombuchas. The sweet potato leaves' tea and kombucha phenolic profile performed by LC-MS consisted of: quinic, chlorogenic, dicaffeoylquinic, ferulic, and azelaic acids; rutin, and a compound derived from quercetin. These compounds present several health benefits, suggesting that producing tea and kombucha from sweet potato leaves is an excellent opportunity to add value to this material.
Determination of the Androgen Receptor Status of Disseminated Tumor Cells in Primary Breast Cancer Patients
Authors: Natalia Krawczyk , Jäger Bernadette , Martina Piperek-Jäger , Cristina Lopez-Cotarelo Rodriguez-Noriega , Melissa Neubacher , Maggie Banys-Paluchowski , Franziska Meier-Stiegen , Hans Neubauer , Dieter Niederacher , Eugen Ruckhäberle , Svjetlana Mohrmann , Jürgen Hoffmann , Thomas Kaleta , Irene Esposito , Tanja Fehm 
Published: May 2023

Abstract

Purpose Androgen receptor (AR) can serve as a new therapy target since it was shown to play a proliferative role in several BC subtypes. Moreover AR-positivity has been suggested to reflect the metastatic potential of tumor cells in some of BC subtypes. The aim of this study was to determine the AR-expression on disseminated tumor cells (DTCs) as a surrogate marker of minimal residual disease (MRD) and potential precursor of metastasis in early BC. Methods Bone marrow (BM) aspirates from 62 DTC-positive early BC patients were included into this study and analysed by immunofluorescence triple staining for the presence of AR-positive DTCs. CK-positive, CD45-negative cells that contained an intact nucleus (DAPI-positive) were identified as DTCs. The AR expression of the PT was assessed by immunohistochemistry on formalin-fixed, paraffin-embedded (FFPE) tumor sections from core biopsies and surgical specimens. Results AR-status of DTCs could be determined in 21 patients and 9 of those (43%) had AR-positive DTCs. No correlation could be found between positive AR-status of DTC and any of the clinicopathological factors. AR expression of DTC and corresponding PT showed a concordance rate of 33%. No significant correlation could be observed between the AR status of the PT and any of the established prognostic factors for BC. Conclusion AR-positive DTCs can be detected in BM of early BC patients with a marked discordance of the AR status between DTCs and corresponding PTs. The clinical significance of these findings needs further investigation.