bluuebunny/crossref_metadata_2025_split huggingface dataset:
Observation of a 10-day Therapeutic Fast of An Obese Female
Authors: Purvis Alison , Ruddock Alan , Hembrough Dave , Ranchordas Mayur , Claxton David 
Published: March 2023

This case study features an observation of an obese female (body mass index ≥ 30 kgm-2) who undertook a 10-day water and electrolyte fast with the aim of reducing body fat and improving physiological markers of health. A comprehensive range of physiological measures (body mass, girths, blood pressure, body fluid balance, urine osmolarity, and resting oxygen consumption), mood indicators, metabolic markers (blood ketones, blood glucose, breath ketones, urine ketones), and measurement of intake and expenditure (fluid intake, food intake, nutritional macros, steps taken, and estimated movement caloric expenditure) were measured before, during and after the fasting period to determine the effectiveness of undertaking extended therapeutic fasting for improving markers of health and weight loss. Body mass on the first day of the fast was 95.1 kg and on the tenth and final day of the fast it was 88.1 kg representing a loss of 7 kg at a rate of 0.7 kg·day1. Mean body mass in the 15 days after the fast was 88.3 ± 0.4 kg. This case study is novel in reporting key physiological and psychological markers over a 10-day fast, and in pre- and post-fast periods. A 10-day fast appears to be a safe and effective method of weight loss, mood is positively affected by fasting and weight loss from fasting can be sustained providing care is taken in the approach to post-fasting nutrition.
Enseñanza de español L2 para migrantes haitianos: Metodología participativa
Authors: Markovits Jennifer , Abarca Cristian 
Published: March 2023

El siguiente artículo describe una experiencia de metodología curricular participativa para la realización de material didáctico para la enseñanza de español como L2 para la comunidad haitiana en Chile. Para lograr esta finalidad se diseñó y luego se aplicó un cuestionario sobre percepciones de conocimiento lingüístico (CPL) para medir las creencias de 42 migrantes adultos haitianos de la región de Valparaíso con respecto a su conocimiento del español. De manera paralela, se aplicó el Test de nombramiento multilingüe MINT (Gollan et.al, 2012) para medir cuantitativamente el conocimiento léxico de la lengua meta. Los resultados de ambos instrumentos revelaron las necesidades subjetivas y objetivas con respecto del español de la comunidad que se utilizaron para llegar al resultado esperado que fue la construcción de un manual de español como L2 con un enfoque comunicativo e intercultural.
Una PROPUESTA INNOVADORA PARA LA ENSEÑANZA DEL INGLÉS COMUNICACIONAL EN UNA UNIVERSIDAD ARGENTINA. SUGERENCIAS DE MEJORAS A PARTIR DE LA OPINIÓN DE ESTUDIANTES
Authors: Aguirre Céliz Cecilia , Rivarola Marcela , Domínguez María Belén 
Published: March 2023

En este trabajo se comenta sobre una experiencia inédita que se llevó a cabo en la Facultad de Ingeniería y Ciencias Agropecuarias de la Universidad Nacional de San Luis, Argentina. Esta experiencia consistió en el montaje e implementación de “Inglés Comunicacional” como asignatura electiva para los estudiantes de ingeniería de esta alta casa de estudios. Luego de dos años de intensa investigación para fundamentar la necesidad del inglés comunicativo en las carreras de ingeniería, y de dos años más de tareas del diseño de la asignatura en cuestión, esta se implementó como prueba piloto en el segundo semestre del año 2021. El objetivo de este trabajo es retomar las opiniones de los estudiantes respecto a la metodología de aula invertida, el uso de las tecnologías para el dictado de la asignatura, el cumplimiento de sus expectativas iniciales y sus sugerencias para mejorar esta propuesta pedagógica. Luego de haber analizado las respuestas de los estudiantes, se proponen una serie de mejoras para la próxima implementación en el segundo semestre del año 2022.
Palabras clave: universidad, enseñanza, lengua inglesa, innovación pedagógica
CUENTO DE LUZ NEGRA REALIZADO POR EL ALUMNADO DEL CICLO FORMATIVO DE EDUCACIÓN INFANTIL IMPLEMENTANDO METODOLOGÍAS ACTIVAS
Authors: Lumbreras Gil Mª Pilar 
Published: March 2023

Rodari (1973) defendía la necesidad de que la imaginación tuviera un puesto en el proceso educativo, la confianza en la creatividad infantil y el valor liberador que puede tener la palabra. El alumnado del Ciclo Formativo de Educación Infantil (futuras educadoras y educadores infantiles que trabajarán en la etapa de 0 a 6 años) desarrollan cuentos con la técnica de luz negra que se representarán para los niños y las niñas de Educación Infantil. Es un proyecto combinado con la metodología de aprendizaje-servicio en el que tienen que implicarse en todos los pasos: escribir la historia teniendo en cuenta la técnica de luz negra y al público receptor, construir los personajes, organizar los ensayos y representar los cuentos. Es un proceso muy motivador por su carácter práctico y por el feedback de los niños y las niñas. El teatro negro atrapa la atención con su magia e ingenio.
Evaluation of ATBS Polymers for Mangala Polymer Flood
Authors: Shankar Vivek , Zagitov Robert , Shekhar Sunit , Gupta Abhishek Kumar , Kumar M. Suresh , Kumar Ritesh , Veerbhadrappa Santhosh , Nakutnyy Petro 
Published: March 2023

Summary
Mangala field has been under polymer flood since 2015. The polymer flood has been more successful in accelerating recovery compared to waterflood. As the flood matured, field performance indicated that part of the injected polymer was degrading in the reservoir. Laboratory studies and polymer samples collected from the reservoir suggest that the most likely reason for the degradation is increased hydrolysis due to thermal aging. This degradation compels higher dosing of polymer to make up for the lost viscosity and increases operating costs. Polymer precipitation in the reservoir may also lead to loss of reservoir permeability.
Literature surveys and preliminary laboratory studies showed that polymers with acrylamide-tertiary-butyl-sulfonic acid monomer units (referred to as ATBS polymers) could be a suitable option for Mangala. To evaluate the hypothesis, the team did a series of laboratory and coreflood studies. The studies include accelerated thermal ageing, rheology, dynamic adsorption, injectivity, waterflood with fresh and degraded samples, and compatibility studies with topside chemicals. Two hydrolyzed polyacrylamide (HPAM) polymers with different degrees of hydrolysis (DOH) and two ATBS polymers were evaluated. The selected ATBS polymer was then tested for compatibility with surface topside chemicals.
The studies show that the classic 20 to 25% DOH HPAM suffers viscosity degradation and possible precipitation in Mangala reservoir conditions. ATBS polymers and a lower DOH HPAM provide superior results to the incumbent HPAM with an acrylamide (AM) (86)-ATBS (14) copolymer providing the best results. ATBS polymers were especially resistant to cloudpoint lowering and provide some superiority in shear degradation. The ATBS monomer was resistant to hydrolysis during the period of testing. Contrary to the published literature, ATBS polymers showed higher adsorption and their propagation through cores required a higher pressure drop. ATBS polymer seemed to plug a low-permeability section of the core stack. All polymers reach their peak viscosity at 30 to 40% hydrolysis and decline sharply after 40%, but viscosity and cloudpoints measured during accelerated aging are possibly conservative. A large-scale pilot of ATBS injection in Mangala is under way to validate the laboratory test results.
ATBS polymer can be a suitable polymer for some layers of Mangala with a high residence time and permeability. The choice is driven by the economics of the incremental cost of ATBS for the benefits it offers. In some sands with shorter interwell spacing, a lower DOH HPAM may be a more cost-effective solution.
The study results in this paper provide insights to operators to understand the reservoir performance of existing polymer floods and plan for future polymer floods.
Research on elliptic curve crypto system with curves Bitcoin- SECP256k1,NIST256p,NIST521p and LLL
Authors: Ulla Mohammed Mujeer , Sakkari Deepak S 
Published: March 2023

Abstract
Very recent attacks like ladder leak demonstrated feasibility to recover private key with side channel attacks using just one bit of secret nonce. ECDSA nonce bias can be exploited in many ways.Some attacks on ECDSA involve complicated Fourier analysis and lattice mathematics. In this paper will enable cryptographers to identify efficient ways in which ECDSA can be cracked on curves NIST256p,SECP256k1,NIST521p and weak nonce, kind of attacks that can crack ECDSA and how to protect yourself. Initially we begin with a ECDSA signature to sign a message using private key and validate the generated signature using the shared public key. Then we use a nonce or a random value to randomize the generated signature.Every time we sign, a new verifiable random nonce value is created and way in which the intruder can discover the private key if the signer leaks any one of the nonce value. Then we use Lenstra–Lenstra–Lovasz (LLL) method as a black box, we will try to attack signatures generated from bad nonce or bad random number generator(RAG) on NIST256p,SECP256k1 curves. The analysis is performed by considering all the three curves for implementation of Elliptic Curve Digital Signature Algorithm (ECDSA).The comparative analysis for each of the selected curves in terms of computational time is done with leak of nonce and with Lenstra–Lenstra–Lovasz method to crack ECDSA.The average computational costs to break ECDSA with curves NIST256p,NIST521p and SECP256k1 are 0.016,0.34,0.46 respectively which is almost to zero depicts the strength of algorithm. The average computational costs to break
Association between gut microbiota and erectile dysfunction: a two-sample Mendelian randomization study
Authors: Chen Wenkang , Zhang Yijing , Zou Hede , Li Bolin , Liu Hanfei , Wang Ruikun , Zhao Jiayou 
Published: March 2023

Abstract
Objective: Several observational studies have recently been reported to suggest an association between gut microbiotaand erectile dysfunction（ED）. However, the causal relationship between gut microbiota and erectile dysfunction remains unclear. Therefore, the aim of this study was to clearly elaborate the causal relationship between gut microbes and erectile dysfunction.
Methods: A two-sample Mendelian randomization study was conducted with summary statistics of the gut microbiota from the largest genome-wide association study Meta-analysis conductedby the MiBiogen Consortium (n=13, 266). ED patients’ information was collected from the European ancestry (6175 cases vs 217,630 controls). The causal relation between gut microbiota and ED was investigated using inverse variance weighting (IVW), MR-Egger, weighted median, weighted model, and MR-PRESSO. Reverse Mendelian randomization（MR） analysis was performed on bacteria found to be causally involved in erectile dysfunction in forward Mendelian random analysis. Cochran's Q statistic was employed to quantify the heterogeneity of instrumental variables.
Results: IVW estimate indicates that Lachnospiraceae(OR=1.26, 95%CI=1.06 to 1.5, P=0.008), Oscillibacter(OR=1.2, 95%CI=1.04 to 1.39, P=0.016), Tyzzerella3(OR=1.14, 95%CI=1.02 to 1.27, P=0.024) and Erysipelotrichaceae UCG003(OR=2.05, 95%CI=1.54 to 2.56, P=0.006) were risk factor for ED. Sensitivity analyses were performed to verify the causal relations obtained by MR. The results of Cochran’s IVW Q test showed no significant heterogeneity of these IVs. Based on the results of the inverse MR analysis, no obvious causal effect of ED on intestinal flora was found. There was no significant heterogeneity found for instrumental variables or horizontal pleiotropy.
Conclusions: This two-sample MR study found that gut microbes including family Lachnospiraceae、genus Erysipelotrichaceae UCG003、genus Oscillibacter、genus Tyzzerella3 were causally associated with ED. Further studies are needed to clarify the mechanism of gut microbes’s pathogenesis role in ED.
Effect of Probiotic Lactic Acid Bacteria and Citric Acid on Performance, Serum Biochemistry, Organ Weight and Carcass Characteristics of Broiler Chicks
Authors: Heidary Arezoo , de Neeff Diane Valganon , Shahbazi Hosseinreza , Seidavi Alireza , Oliveira Carlos Augusto Fernandes 
Published: March 2023

Abstract
In this work, the effects of the addition of probiotics and citric acid to drinking water on performance, serum biochemistry, relative organ weight and carcass characteristics of broiler chicks were evaluated. Two hundred forty-one-day-old chicks (female Ross 308) were divided into 6 groups and submitted to 6 different treatments for 42 days, with 4 replicates in each treatment, with 10 birds in each. Drinking water was supplemented with a commercial probiotic mixture (Lactofeed®, Takgene Co, Iran) at 0 (control), 0.1 g/L (day 1 to 21) and 0.05 g/L (day 22 to 42), and citric acid at concentrations of 0 (control), 0.2 and 0.4% (day 1 to 42). Compared with controls, no significant changes (P > 0.05) were found in the performance, serum biochemistry parameters and relative organ weights of broiler chicks receiving water supplemented with probiotics or citric acid after 42 days of experiment. However, a significant (P < 0.05) interaction effect was observed between probiotic and citric acid supplementation on feed intake within the first 21 days. Moreover, chicks receiving water supplemented with probiotic and 0.2% of citric acid had highest (P < 0.05) body weight at 42 days of age, as well total carcass weight. Results indicate that water supplementation with Lactofeed® and 0.2% of citric acid are promising strategies to enhance growth performance of broilers.
The role of nano-chelated iron on anatomical and biochemical characteristics and concentration of mineral nutrients in lettuce (Lactuca sativa L.) under cadmium toxicity
Authors: Heydari Roghayeh , kolahi maryam , Kazemi Elham Mohajel , Nosratia Houshang , Movafeghia Ali 
Published: March 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Lipids Productivity of Cyanobacterium Anabaena vaginicola in an Internally Illuminated Photobioreactor Using LED Bar Lights
Authors: Goldoost Hootan , Vahabzadeh Farzaneh , Fallah Narges 
Published: March 2023

Abstract

Concerns over environmental issues exists and desire to decrease of their extent, have directed efforts toward green energy production. Growth behavior of Anabaena vaginicola, was determined in a photobioreator which illuminated internally (IIPBR) using LED bar light. Excessive heat generated in the IIPBR was taken care of by applying a novel air-cooled system. Further note in experimentation was to find favorable cultivation conditions in the IIPBR for A. vaginicola growth and its lipids production capacity. The following results are expressed: 80 µmol photons m-2 s-1 as light intensity, 0.5 g/l as NaNO3, and 120 ml/min as CO2 amount being expressed in terms of aeration rate. The findings were interpreted in terms of a two-component system where the genes encoded to the relevant proteins are present in cyanobacteria and their expressiveness depends on environmental stress. By determining growth rate constant as 0.11 d-1, the productivity in terms of biomass formation was calculated as 202.6 mg L−1 d−1. While rate of lipids production by the test cyanobacterium is 15.65 mg L−1 d−1. Based on total energy used for IIPBR performance, biomass productivity per unit power input equals to 0.74 g W-1 d-1 and this is in favorable position compared with other photobioreactors.
Designing and Development of a DNA Vaccine Expressing HER2/ HER3 and CCL20 as a Novel Candidate for Breast Cancer Vaccine
Authors: Heshmatzad Katayoun , Gholamzad Amir , Gholamzad Mehrdad , Eslami Maryam 
Published: March 2023

Abstract
Background: Breast cancer (BC) is one of the most common diseases among women worldwide and about 2.1 million new cases were diagnosed in 2018. Despite effective treatments such as chemotherapy, radiotherapy and Hormone therapy, BC has been remained as one of the significant reasons of women ‘s death. Recent progress in the new field called as immunotherapy have changed the management of breast cancer completely and plays a major role in the efficacy improvement of BC conventional treatments. One of the significant types of Antigen-specific immunotherapy is DNA vaccine. This type of vaccine is based on designing a plasmid which can encode peptides which are important in cancer immunothrapy. In this study Bioinformatics Tools and In-silico prediction methods were used in order to select the appropriate HER2, HER3, CCL20 sequences and the standard form of the recombinant Vector(PIRES2-GFP). PIRES2-GFP transfection in to HECK 293 T cell line was carried out and its expression was evaluated by fluorescence microscope.
Reuslt: This study results indicated that plasmid containing HER2 and HER3 and CCL20 can be expressd as an fusion protein that can be used as breast cancer vaccine.
Conclusion: Cancer vaccines have led to modest effects in treatment of cancer affected patients due to immunosuppression in micro environment of Tumors. Some strategies have been taken in order to overcome this problem including Optimization of the Antigens and the combination of vaccine with other complementary therapies. The cytokine-based adjuvants such as interleukin-12 (IL-12) have been suggested as one of the most effective enhancing immune responses options.
Plant community compositional stability over 40 years in a Fraser River Estuary tidal freshwater marsh
Authors: Lane Stefanie , Shackelford Nancy A. , Bradfield Gary E. , Denoth Madlen , Martin Tara G. 
Published: March 2023

Abstract

Long-term data sets documenting temporal changes in vegetation communities are uncommon, yet imperative for understanding trends and triggering potential conservation management interventions. For example, decreasing species diversity and increasing non-native species abundance may be indicative of decreasing community stability. We explore long-term plant community change over a 40-year period through the contribution of data collected in 2019 to two historical datasets collected in 1979 and 1999 to evaluate decadal changes in plant community biodiversity in a tidal freshwater marsh in the Fraser River Estuary in British Columbia, Canada. We examine whether characteristic plant assemblages are consistent over time, whether alpha (α) and beta (β) diversity change within and between assemblages, and whether associated indicator species change. We found that plant assemblages were characterized by the same dominant indicator species, but most other indicator species changed, and that overall α-diversity decreased while β-diversity increased. Further, we found evidence for plant assemblage homogenization through the increased abundance of non-native invasive species such as yellow flag iris (Iris pseudacorus) and reed canary grass (Phalaris arundinacea). These observations may inform concepts of habitat stability in the absence of pulse disturbance pressures, and corroborate globally observed trends of native species loss and non-native species encroachment. Our results indicate that within the Fraser River Estuary, active threat management may be necessary in areas of conservation concern in order to prevent further native species biodiversity loss.
What factors significantly impact Chinese subjective well-being and survival? A longitudinal study using joint modelling
Authors: Qiao Xiaoyan , Nie Huilan , Chen Zhi , Lan Yifu , Zhang Han , Hao Yabin , Webb Lucy , Shi Xin 
Published: March 2023

Abstract
Background

Well-being is the goal that everyone and every country pursues. However, studies of the dependence between time-varying longitudinal measures of subjective well-being and survival outcomes are still scarce. The aim of this study was to conduct joint model analysis of longitudinal data and survival data to explore the influencing factors of Chinese residents' subjective well-being and survival, and to assess the impact of longitudinal dynamic measurements on survival outcomes.

Methods

Based on adult data (≥16) from the China Family Panel Studies obtained between 2010-2018, a spatiotemporal analysis of Chinese subjective well-being levels was conducted and joint modelling was used to analyze subjective well-being. In addition, the individual dynamic survival probability was predicted.

Results

In terms of the demographic characteristics, Han nationality, male, urban living, and married or cohabiting individuals are happier than non-Han, female, rural living, and being single. The older group was happier than the youth group. People with fewer children were happier. In terms of the SES, access to medical insurance, higher education, high social status, high per capita net income of households, large housing area, and ownership of other properties were protective factors for subjective well-being, while being a member in organizations was significantly negative. In terms of the living habits, smokers had worse subjective well-being than non-smokers; Physical exercise could enhance people's subjective well-being and drinking had a positive impact on subjective well-being. In terms of the health status, physical health had a positive impact on subjective well-being, while both hospitalization and chronic diseases had a negative impact on subjective well-being. As regards the survival outcomes, the study demonstrated that each unit increase in subjective well-being was associated with a 13% reduction in the risk of death.

Conclusions

In general terms, population subjective well-being in China tends to decline over time. This study aims to provide powerful evidence and decision-making support for government-level improvements to the subjective well-being of residents and prolong their lifespan.
Application of a novel improved Manhattan distance on bearing fault diagnosis
Authors: Wang Wei , Li Shaohui , Sun Yongjian 
Published: March 2023

Abstract
Rolling bearing is a common rotating machine in industry. Once it is damaged, the industrial machinery associated with it will be affected, and if it is serious, it may threaten life safety. Thus, an effective fault diagnosis method can reduce the occurrence of accidents. In the light of the principle of Manhattan distance and symmetrized dot pattern (SDP), the Manhattan distance is improved and a new variable is obtained. It is used as characteristic parameter to diagnose fault type. Firstly, sample data of rolling bearing under various working conditions is extracted, and it’s split into 10 pieces of data. Then, through the SDP principle, the equal part of the data is became a picture, and the symmetrical image in polar coordinate system is obtained. After binarization of the SDP image, the local area of the binarized SDP image is selected, and the mean value matrix of the local matrix is computed, and salt and pepper denoising is carried out for each local matrix and mean value matrix, and the maximum characteristic value of average value array after salt and pepper denoising is computed. Finally, local images are corresponded with their average images one by one, and the Manhattan distance between them is calculated. According to the feature method, the improved new value is obtained by linear transformation, which is called the improved Manhattan distance, and then the average value is obtained. Through the experimental data to verify whether the method can effectively distinguish the fault type.