bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suppressive effect of crepidatin on stemness of lung cancer cells
Authors:   , Bhummaphan Narumol 
Published: September 2023

Cancer stem cells (CSCs) have been perceived as rare populations driving cancer progression, metastasis, and drug resistance in leading cancers. The discovery of new compounds that attenuates CSCs’ properties is crucial for enabling advances in novel therapeutics to limit recurrence. We have reported the CSC-suppressing activity of chrysotoxine and crepidatin, two bibenzyl compounds isolated from Dendrobium pulchellum. Both chrysotoxine and crepidatin displayed suppression in CSC-like phenotypes, as determined in CSC-rich populations and primary CSCs in 3D culture and extreme limiting dilution assay. Individual treatment of chrysotoxine and crepidatin also affected the expression of CSC markers and associated proteins concomitantly decreased known CSCs markers. Importantly, chrysotoxine was shown to suppress active Src/Akt signal and intern depleted Sox2-mediated CSC. Our findings indicate a novel CSC-targeted role of chrysotoxine and its regulation by Src/Akt and Sox2, which may be exploited for the cancer treatment. At the same time, crepidatin showed the highest potency to kill human lung cancer cells. Crepidatin was accomplished as a drug targeting model for lung cancer stem cells. Crepidatin also suppressed CSC-like phenotypes, as determined by CSC-rich populations in 3D culture, clonogenic assay and extreme limiting dilution assay, when compared to cisplatin. The treatment of crepidatin showed dramatic suppression in the CSCs in lung cancer cells, as verified by several CSC phenotype assessments and CSC markers. In addition, crepidatin also suppressed epithelial to mesenchymal transition (EMT) markers and inhibited migratory behavior of CSCs. Importantly, we confirmed the CSC-targeted activity of crepidatin in CSC-rich primary lung cancer cells. The compound dramatically inhibited the formation of tumor spheres of primary lung cancer cells. Finally, we confirmed the effect of crepidatin on the aggressive stem‐like and invasive phenotype in lung cancer cells, where in vivo growth and aggressiveness of crepidatin and cisplatin treated cells were analyzed using the chorioallantoic membrane (CAM) assay. The results revealed that crepidatin significantly reduced tumor growth in lung cancer cells, when compared to nontreated cells, whereas the tumor sizes have been significantly increased in cisplatin treatment. These findings document a novel pharmacological action and establishes the underlying mechanism of chrysotoxine and crepidatin in negatively regulating CSC-like phenotypes and sensitizing resistant cancer cells. Therefore, the findings from this study would provide important insights that facilitate the further investigation and development of the two natural compounds, chrysotoxine and crepidatin, for CSC-targeted approaches.
Comparison of microtensile bond strength of universal adhesives utilizing various application methods: an in vitro study
Authors:   , Jeansuwannagorn Suthatta 
Published: September 2023

Purpose: To investigate microtensile bond strength (μTBS) of a conventional three-step etch and rinse, and universal adhesive systems with etch and rinse mode utilizing various application methods at 24 hour and after 6 month water-­storage.

Material and methods: Seventy-­two extracted human third molars were cut to expose a flat dentin surface. Optibond FL (OFL; Kerr, USA), Single Bond Universal (SU; 3M ESPE, USA), Clearfil Universal Bond Quick (CU; Kuraray Noritake Dental Inc., Japan), G-­Premio BOND (GP; GC Corporation, Japan), Prime&Bond Universal (PB; Dentsply, Germany) were used in etch and rinse mode following the manufacturers’ instruction and double application method. Subsequently, composite resins were constructed on prepared dentin and then sectioned into 1x1x8 mm3 stick to be tested after 24 hours and 6 months of water-­storage by microtensile bond strength (μTBS) testing. The data of universal adhesives were analyzed with two-­way ANOVA (p = 0.05). Additionally, the μTBS values were obtained and analyzed with one-­way ANOVA followed by a Tukey Post Hoc test and a Pair T-­test.

Results: The µTBS of universal adhesives were not significantly increased when using double application method compared to manufactures’ instruction at 24 hr (p = 0.652) and after 6 month water storage (p = 0.173). In contrast, kind of adhesives had statistically significant influence on µTBS at 24 hr (p < 0.001) and after 6 month water storage (p < 0.001). Optibond FL, Single Bond Universal Adhesive when applied in double application methods, G-Premio BOND when following the manufacturer’s instruction, and appling in double application method had statistically significantly decreased µTBS after 6 month water-storage.

Conclusions: Double application of universal adhesives did not improve the bond strength of tested universal adhesives at 24 hours and after 6 months of water storage. Single Bond Universal used following manufacturer’s instruction, Clearfil Universal Bond Quick and Prime&Bond Universal used both following manufacturer’s instruction and modified double application method, had stable dentin microtensile bond strength, while Optibond FL and G-Premio Bond used both techniques had deceased bond strength after 6 months of water storage.
Effects of Monovalent Ions on Low Salinity Waterflooding in Shaly-sandstone Reservoir
Authors:   , Yomchan Ativish 
Published: September 2023

Multi-component Ion Exchange (MIE) was proved to be a major mechanism occurred during Low Salinity Waterflooding (LSWF). Reduction of both total salinity and concentration of divalent ions of injected water together with a presence of monovalent ion results in dissolution of divalent ions linking between rock surface and oil. As a consequence, oil is liberated from rock surface.

This study aims to study effects of monovalent ions in MIE mechanism. Stirring test is performed and results show that ion with less hydration radius tends to be more active in MIE; hence, potassium ion and cesium ion illustrate potential in oil recovery mechanism. Then, the best three water formulations can be selected to perform imbibition and coreflood tests and both tests show results in the same direction. Pure cesium ion is very fast in MIE mechanism while pure potassium ion with a few water molecules can also function well in oil recovery. However, its mobility is less than cesium ion. The combination of cesium-sodium-calcium at 20-40-40 tends to be similar results of pure potassium ion. In comparision, Pure cesium ion is the best water formulation enhacing recovery factor up to 0.26. However, complexometric titration result shows remarkable contrast to the stirring test that both cesium ion and potassium ion tend to displace magnesium ion better than calcium ion. In conslusion, this study has already proved the theory regarding the hydrate of monovalent ion.
PM10 and PM2.5 concentrations in Bangkok over 10 years and implications for air quality
Authors:   , Thongthammachart Tin 
Published: September 2023

Atmospheric particulate matter particles with a diameter less than 10 micron (PM10) and less than 2.5 micron (PM2.5) have been adverse human health effect. Ambient air pollution data in Central Business District (CBD) of Bangkok monitored by Pollution Control Department and Bangkok Metropolitan Administration were collected over ten years in Bangkok from 2008 to 2017. Trend of the particulate matter concentrations data were investigated over ten year for implications of activities related to air pollution. Daily average air pollution data between year 2015 and year 2017 were used as the dependent variables to develop mathematic models with multiple linear regression (MLR) technique for forecasting PM2.5 concentrations in non-monitored areas. The predicted values of PM2.5 concentrations were validated by various statistical performance indicators. The Q-Q plots of the measured and predicted values of both selected ambient monitoring data and roadside monitoring data had significant correlations, with R2= 0.88 and 0.96, respectively. The non-carcinogenic health risk assessment of PM10 and PM2.5 was quantified as the hazard quotient (HQ) from both the measured and predicted data. The risk areas and HQ were compared using the Inverse Distance Weighting (IDW) interpolation technique and illustrated as GIS-based maps. The highest HQ value was discovered in the southern area of the CBD. The risk area could be used for greater precaution propose of public health awareness.
An empirical study of human capital on economic growth: An investigation using three indices of human capital
Authors:   , Fu Shan 
Published: September 2023

This research reexamines the augmented Solow’s growth model to find the relationship between human capital and economic growth by using three different proxies for human capital. The first proxy of human capital is the proportion of labor force with secondary education that has been widely used in many studies. PISA score is the second proxy to human capital. It is regarded as being quality of education because this standardized test score could directly reflect the cognitive ability of individuals. The third proxy is the human capital index constructed by Penn’s World Table 9.0 that included both quantitative and qualitative aspects of human capital. The study also includes physical capital into the estimation of economic growth. The equilibrium growth model was firstly estimated based on the cross-section data of 21 middle-income countries and 36 high-income countries from 2010 to 2016. An alternative approach of estimation is the panel fixed effects model of 46 countries from 2010 to 2013 to allow different country-specific effects. Consistently with most studies, our result indicated that both physical capital and human capital are crucial factors to drive economic growth. The study found that human capital has played a positive role in economic growth in all proxies. However, it has been found that the most significant proxy is an index from Penn’s. An important implication of the findings is to confirm that human capital indicator should not only deal with a number of highly educated workers but it should also cover features in labor productivity improvement in an economy.
The role of histone methylation of H3K4 in polarization of macrophages stimulated by immune complex and lipopolysaccharide and its therapeutic potential in sepsis
Authors:   , Ruenjaiman Vichaya 
Published: September 2023

Macrophage plasticity is a process that allows macrophages to switch between two opposing phenotypes based on differential stimuli. Functional plasticity of macrophages contributes to disease pathogenesis such as cancer, metabolic diseases, autoimmune diseases and systemic infections. Epigenetics plays an important role in regulating this process. Macrophages stimulated with lipopolysaccharide (LPS) (M(LPS)) produce high level of pro-inflammatory cytokines such as IL-12, TNFα and IL-6 and low level of anti-inflammatory cytokine IL-10, while macrophages stimulated with LPS in the presence of immune complex (IC) (M(IC)) produce high level of IL-10 and low level of IL-12. In this study, we investigated the plasticity of M(LPS) to become M(IC) in vitro and compared the active histone mark (trimethylation on lysine 4 of histone 3 (H3K4me3)) between M(LPS) and M(IC) using murine bone marrow-derived macrophages. We found that macrophages exhibited functional plasticity from M(LPS) to M(IC) upon re-polarization after two days of resting in vitro. Phosphorylation of p38, p44/42, p65 and Akt in M(IC) re-polarized from M(LPS) was similar to M(IC) stimulated from resting macrophages. To obtain the epigenetic profiles of M(LPS) and M(IC), global H3K4me3 enrichments in both activated macrophages were compared. M(LPS) and M(IC) displayed marked differences in genome-wide enrichment of H3K4me3. M(IC) showed increased global enrichment of H3K4me3 whereas M(LPS) decreased enrichment when compared to unstimulated macrophages. Furthermore, M(IC) exhibited high H3K4me3 enrichment in all cis-regulatory elements. At individual gene, increased H3K4me3 enrichments were observed in the promoters of known genes associated with M(IC) including Il10, Cxcl1, Csf3 and Il33 when compared with M(LPS). Finally, to evaluate the therapeutic application of M(IC), we investigated the impact of M(IC) on systemic immune response by adoptive transfer of M(IC) in LPS-induced endotoxemia model. Cytokine profiles revealed that mice receiving an adoptive transfer of M(IC) acutely reduced the serum inflammatory cytokines IL-1β and IL-p12p70. This study highlighted the importance of epigenetics in regulating macrophages activation and functions of M(IC) that may influence macrophage plasticity and the potential therapeutic use of macrophage in vivo.
Extraction of spent green tea leaves as bioactive-compound source
Authors:   , Yannarat Monsichar 
Published: September 2023

Spent green tea leaves (SGTL) are a major contributor to the high cost of commercial tea products because of the large amount of leftover and the cost of disposal. This research investigates on the solvent and time for the extraction of SGTL that provides good yield of potential compound and to separate bioactive compounds as enrich-fractions and pure compounds. The obtained fractions were evaluated for antioxidant, antityrosinase, anticholinesterase and plant growth regulator activities. Caffeine was found in all samples. Among five extracts, the dichloromethane extract revealed high caffeine content (92.8 %peak area), while the methanol extract showed the highest five-catechin content (64.5 %peak area). Further separation of the latter by liquid-liquid extraction, the aqueous phase was extracted with ethyl acetate to gain high catechin family content (EGC, C, EC, EGCG, ECG) fraction with low caffeine. Four subfractions were separated from enrich-catechin family fraction. The enrich-caffeine and enrich-catechin family fractions displayed remarkable biological effects. Caffeine, gallic acid and ECG were bioactive compounds responsible for the biological activity in SGTL. Bioactive compound were isolated from SGTL showed bilogical activity including antioxidant, antityrosinase, anticholinesterase and plant growth regulation. SGTL was proved to be an excellent source of bioactive compounds. The enrich fractions served as prospective materials for the exploration of new multi-functional food and drug formulations.
Metabolic engineering of synechococcus elongatus pcc 7942 for mycosporine-2-glycine production under salt stress condition
Authors:   , Pingkhanont Panwad 
Published: September 2023

ไมโครสปอรีน-ไลก์ อะมิโน แอซิด (MAAs) เป็นกลุ่มของสารเมแทบอไลต์ทุติยภูมิ สังเคราะห์โดยจุลินทรีย์และสาหร่ายหลายชนิด สารกลุ่มนี้มีหน้าที่สำคัญในการคัดกรองรังสียูวี และยังมีหน้าที่อื่นๆ ได้แก่ การต้านอนุมูลอิสระ ปกป้องเซลล์จากภาวะเครียด และควบคุมการออสโมซิส โดยไมโครสปอรีน-2-ไกลซีน (M2G) เป็นหนึ่งในสารกลุ่ม MAAs ที่มีความสามารถในการต้านอนุมูลอิสระมากกว่าสารอื่นๆ ในกลุ่มเดียวกัน ดังนั้นจึงเป็นที่สนใจที่จะเพิ่มการผลิต M2G เพื่อนำไปประยุกต์ใช้ในอุตสาหกรรมความงาม และเวชสำอาง ในการศึกษานี้ประสบความสำเร็จในการแสดงออกของยีนในชีวสังเคราะห์ M2G (Ap3858-3855) จาก Aphanothece halophytica ในไซยาโนแบคทีเรียน้ำจืด Synechococcus elongatus PCC 7942 จากการวิเคราะห์การถอดรหัสของยีน Ap3858-3855 พบว่า ยีนทั้ง 4 ยีนนี้เป็น monocistronic mRNA heterologous expression ของยีน Ap3858-3855 ใน S. elongatus พบว่าทุกยีนมีการแสดงออกมากขึ้นภาวะเครียดจากเกลือ NaCl ยีน Ap3857 แสดงออกเพิ่มมากที่สุด 2.39 ± 0.25 เท่า และภายใต้ภาวะเครียดจากเกลือ KCl ยีน Ap3856 แสดงออกเพิ่มมากที่สุด 3.70 ± 0.35 เท่า ระดับการแสดงออกของยีนที่เกี่ยวข้องกับภาวะเครียดออกซิเดชั่น (sodB, cat และ tpxA) ในเซลล์แสดงออก พบว่าภายใต้ภาวะเครียดจากเกลือ NaCl มีเพียงยีน tpxA แสดงออกมากขึ้น 4.58 ± 0.58 เท่า ขณะที่ภาวะเครียดจากเกลือ KCl กระตุ้นการแสดงออกของ sodB และ tpxA 4.09 ± 0.86 และ 11.81 ± 1.02 เท่า ตามลำดับ ส่วนการแสดงออกของยีนที่เกี่ยวข้องกับสารออสโมไลท์ ได้แก่ Synpcc7942_0808 และ Synpcc7942_2522 พบว่าในเซลล์แสดงออกที่เจริญภายใต้ภาวะเครียดจากเกลือ NaCl มีเพียงยีน Synpcc7942_0808 มีการแสดงออกมากขึ้น 3.89 ± 0.30 เท่า และภายใต้ภาวะเครียดจากเกลือ KCl พบว่า Synpcc7942_0808 และ Synpcc7942_2522 แสดงออกมากขึ้น 9.40 ± 0.88 และ 3.52 ± 0.29 เท่า ตามลำดับ จากตรวจสอบการแสดงออกของโปรตีน โดยวิธีเวสเทิร์น บลอท พบแบนจำเพาะของโปรตีนเป้าหมาย คือ DDG-synthase (ถอดรหัสจากยีน Ap3858) โดยในเซลล์แสดงออกที่เจริญภายใต้ภาวะเครียดจากเกลือ NaCl มีการแปลรหัสได้โปรตีนมากขึ้นถึง 7.20 เท่า ส่วนการวิเคราะห์สารเมแทบอไลต์ พบว่ามีการสะสมของ M2G เพิ่มขึ้นภายใต้ภาวะเครียดจากเกลือทั้งสองชนิด โดยมีการสะสมของ M2G เพิ่มมากที่สุดภายใต้ภาวะเครียดจากเกลือ NaCl เมื่อเติมเซอรีน 7.35 ng/mgFW
Perceptions, expectations, and satisfaction of patients undergoing dental implant therapy in the Faculty of Dentistry, Chulalongkorn University
Authors:   , Sermsiripoca Khunsiri 
Published: September 2023

The aims of this study were to evaluate patient’s perception before and after receiving dental implant therapy and to investigate patient’s perception, expectation, and satisfaction of dental implant treated by postgraduate dental students and faculty members. This study was designed with a quantitative cross-sectional technique. The data were collected by 3 questionnaires, from patients who intended to receive dental implant treatment during September 2017 to February 2019. Two hundred and fifty participants participated in this study. Most of them were female, aged 55-64 years, graduated bachelor’s degree, and facial and teeth appearance did not affect their career. The proportion of monthly income was quite equally between groups. Most of participants were treated by faculty members and most likely to receive only 1 dental implant placement without bone augmentation. Most common site for implanting was posterior region. The perceptions after dental implant insertion were generally more accurate than prior treatment perceptions, except the higher level of agreement with statement “Dental implant therapy is appropriate for all patients.” after the treatment. In addition, the expertise of dentist significantly affected patient’s perceptions toward dental implant therapy. In conclusion, there were statistically significant differences between pre- and post-treatment perception of patients in almost all statements. Most participants improved their realistic perception after dental implant treatment. However, some patients still had improper perception and unrealistic expectation. Therefore, the appropriate information about dental implant including surgical procedure, post-operative difficulties, and preparation for maintenance program should be more intense provided to prevent the wrong conception, which might affect their satisfaction to the treatment outcome.
Comparison Of Single-Dose And Multi-Dose Antibiotic Prophylaxis In Orthognathic Surgery: A Retrospective Cohort Study
Authors:   , Pochana Teeraphat 
Published: September 2023

Objective: (1) to compare the prophylactic effect of a single versus a multiple dose antibiotic regimens and (2) to determine factors related to surgical site infection in orthognathic surgery. Materials and methods: Medical records of patients underwent orthognathic surgery in the Faculty of Dentistry, Chulalongkorn University between 2014 and 2016 were extracted for medical data including demographic data, American Society of Anesthesiologists (ASA)-classified past medical history, orthognathic surgical procedures and complications, prophylactic antibiotic regimen, and surgical site infection. The records also contained 30-day follow-up information, according to the Centers for Disease Control and Prevention (CDC) diagnostic criteria for surgical site infection. Results: Of 168 patients, three developed signs of infection and four presented maxillary sinusitis. In the infected group, one received 1.2g single-dose intravenous amoxicillin-clavulanate at induction while the remaining two patients were administered with 2 million units intravenous penicillin G every 4 hours perioperatively and one out of two received 1g oral amoxicillin twice a day for a week postoperatively. No significant association was found between types of antibiotic prophylaxis and surgical site infection (p = 0.472). Prolonged operation time significantly increased the risk of infection (p = 0.030). There was no significant difference in infection among other factors including age, gender, ASA score, smoking behavior, orthognathic surgical operations, blood loss, blood transfusion, bone grafting and bad split. Moreover, there was no significant association between contributing factors related to surgical site infection and postoperative maxillary sinusitis. Conclusion: The results suggest that the single-dose antibiotic prophylaxis can be sufficient in prevention of surgical site infection. Prolonged operation time increases the risk of infection.
TLR3 signaling modulates immunomodulatory property of human periodontal ligament stem cells.
Authors:   , Chaikiawkeaw Daneeya 
Published: September 2023

Toll like receptor 3 (TLR3) is a member of TLR family functions to recognizes double-stranded RNA (dsRNA) produced by positive-strand RNA viruses and DNA virusesand generate signaling that stimulate body immune system against virus infection. However, recent evidence suggests that TLR3 signaling may also play a role in initiating the immunosuppressive property of the cells. Study on skin regeneration has shown the anti-inflammatory responses of keratinocytes required for wound healing andthe increasing number of hair follicle formation after TLR3 signaling was activated by fragment of RNA released from damaged cells. Moreover, activation of TLR3 signaling have shown to elicit a higher success rate and lower side-effect for a grafted surgery in animal models. Taken together, TLR3 signaling may play an important role in immunomodulatory property of the cells. The aim of this study is to investigate the involvement of TLR3 signaling on immunomodulatory properties in periodontal ligament stem cells (PDLSCs). PDLSCs expressed TLR3, however, detailed mechanism in which TLR3 signaling modulate immunomodulatory property of PDLSCs has not yet been identified. PDLSCs were established from is periodontal tissue and expressed several multipotent stem cells markers. These cells could also differentiate under appropriate culture conditions. After activation with poly(I:C), a potent activator of TLR3, the expression of interferon-gamma, IDO (indolamine 2,3 dioxygenase) and HLA-G (human leukocyte antigen G), three major molecules that participate in the immunomodulatory function. Addition of bafilomycin A, a chemical inhibitor that prevent the cytoplasmic acidity, could inhibit this inductive property, indicating that activated TLR3 were located in cytoplasmic endosome. RNA silencing approaches could also abolish the inductive effect of poly(I:C) confirming the role of TLR3. Furthermore, the co-culture between poly(I:C)-treated PDLSCs and PBMCs was performed. The results showed the inhibitory effect of PBMCs and the upregulation of FOXO3, a marker of T-regulatory cells support the immunomodulatory role of PDLSCs after activated with poly(I:C). The knowledge from this study will provide more understanding on TLR3-induced immunosuppressive property on PDLSCs that could lead to the clinical application for stem cells therapy in the future.