bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiology of cystic echinococcosis in Al-Najaf province
Authors: Al- Tufaili Rasha Amer Nouri 
Published: August 2023

Cystic echinococcosis (CE) is a zoonosis disease of worldwide distribution caused mainly by the metacestodeEchinococcusgranulosus. In Najaf, its distribution reaches endemic levels. This is results of this study were to investigate the trends in suspected cases of human CE recorded in Al-Sadder Medical City, Al-Hakim General Hospital and Al-Zahraa Teaching Hospital in Najaf city during a period November 2013to September 2014 and to study demographic and clinical characteristics of cases together with epidemiological factors associated with the disease. Clinical records of operated and/or diagnosed patients were reviewed with regard to this disease. 217 cases (68% female; mean age 21-30 years and 32% male; mean 21-30 ) were included in this retrospective study, 79% of which lived in rural areas. Indirect Haemagglutination (IHA) test to detect anti Echinococcusgranulosusantibody, and ultrasonography were used to diagnose the disease. Lung infection is the most common and most cases frequently
Effect of alcoholic extract of urticadioica leaves and Aspirin in experimentally induced prostatitis in mice
Authors: Hasan Huda F. 
Published: August 2023

Urticadioica leaves were powdered and extracted with methanol 70 %. 17 beta-estradiol was used to induce prostatitis. Fifty mice were divided into five groups, The first control group (T1) , The second induction group (T2) was treated by distilled water, the third induction group (T3)was treated with crude extract of Urticadioica leaves 300mg/kg B.W, the fourth induction group (T4) was treated with aspirin 50mg/kg B.W, the fifth induction group (T5)was treated with crude extract of Urticadioica leaves 150mg/kg B.W and aspirin 25mg/kg B.W. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) for induction groups treated with 17 beta-estradiol showed significant decrease as compared with (T1), while the T3,T4 and T5 showed no significant difference as compared with T1. The result of testosterone hormone of (T3) showed significant increase as compared with (T1) and other groups. The results of body and prostate weight for T3,T4 and T5 revealed no significant difference as compared with T1. The fertility index of (T3) was increased as compared with T1 while the T4 was decreased as compared with T1. The histopathological changes after induction of prostatitis revealed hyperplasia of the prostatic glands and infiltration of inflammatory cells, while the T3 revealed the return of the acini to normal shape, and hydropic degeneration in the epithelium of prostatic glands disappeared. The T4 showed decreased hydropic degeneration, few inflammatory cell and epithelial hyperplasia, while the T5 was characterized by few inflammatory cell infiltration and the acini showed very decreased hydropic degeneration. In this study we concluded that the alcoholic Urticadioica extract could markedly treat proststitis by its anti-inflammatory effect with reduce side effect of aspirin such as infertility.
Study the Effects of Ginger (Zingiber officinale) Extract on Serum Lipid in Hypothyroidism Male Rats Induce by Propylthiouracil
Authors: Al-Sharafi Nabeel M. N. , Al-Sharafi Mohammed R.A. 
Published: August 2023

This study was undertaken to investigate the protective role of Ginger against the hypothyroidism induce by Propylthiouracil in male rats on serum lipids.
Eighteen adult male rats were randomly divided into three equal groups (each of six) and treated as follows for four weeks, First group (C) was daily orally gavages with saline solution served as control.. Second group (G1) was daily orally gavages with Propylthiouracil (6 mg/kg B.W.). Third group (G2) was daily orally gavages with Propylthiouracil (6 mg/kg B.W.) in addition to ginger (200 mg/kg B.W.)
Overnight fasting blood samples were collected from the all animals of the experiment at end week of experiment for the determination of serum triiodothyonine (T3), thyroid stimulation hormone (TSH), total cholesterol (TC), triacylglycerol (TAG), very low density lipoprotein-cholesterol (VLDL-C) and high density lipoprotein-cholesterol (HDL-C).
The results showed that the A significant (P<0.05) statistical increase in the activity of TSH was observed in propylthiouracil treated groups G1 (3.72±0.73) and G2 (3.28±0.25) as compared to the control (0.0795±0.003) and A significant (P<0.05) statistical decrease in the activity of T3 in treated (G1) (2.62±0.2) as compared to the control (3.92±0.08). As well as the results show, a significant(P<0.05) elevation in serum TAG, TC and VLDL-C and decrease in HDL-C were observed in animals daily orally gavages with Propylthiouracil 6 mg/kg B.W. (G1) group as compared with control. Oral administration of ginger extract caused a non significant (P>0.05) depression in the elevated TAG and VLDL-C concentration in G2 group compared to G1 and control groups . From these results indicating that the effect of orally administration of propylthiouracil to induced hypothyroidism and induction of Dyslipidemia.
On conclusions, the present study confirmed the beneficial effects of ginger extract as a hypolipidemic effects gains the dyslipidemia due to hypothyroidism.
Epidemiological and Histopathological study of Hepatic Hydatidosis in slaughter animal house in AL- Najaf AL- Ashraf Province
Authors: Al-Shabbani MaythamAskarAlwan 
Published: August 2023

The study was carried out in Al-Najaf slaughterhouse to show the prevalence of hydatid cysts among the slaughtered animals during the period from 1/1/2011 to 31/12/2011 , the slaughtered animals were local breeds , both sex ( male & female ) , different ages and from different region in Al-Najaf province . the hydatid cysts was examined grossly by naked eye and palpation . The study was conducted on 114717 slaughtered animals ( 21799 cattle , 51332 sheep , 41586 goats ) . The morbidity of hydatidosis was 0.93% in cattle , 0.62% in sheep and 0.61% in goats . The gross pathological changes of liver revealed variety necropsy findings represented by enlargement, discoloration to dark and red zones surrounding the hydatid cysts as responses to the inflammatory reaction. Cysts mostly protruded from the surface of the organ and sometimes embedded in the parenchyma. White wall covered the cysts has been observed . The histological changes of livers represented by infiltration of inflammatory cells such as neutrophils, monocytes and phagocytes. Hepatocytes appeared separated from each other and there was vaculation in liver tissue, which contain huge number of kupffer cells .
Effect of ferulic acid on Myocardial Ischemia/Reperfusion Injury In Male Mice.
Authors: AL-Sheibani Bassim I. Mohammad , Hadi Najah Raiesh , Atia Najwan Abdulameer , Yousif Fadhil Ghaly. 
Published: August 2023

Myocardial ischemia–reperfusion represents a clinically relevant problem associated with thrombolysis, angioplasty and coronary bypass surgery. Injury of myocardium due to ischemia–reperfusion includes cardiac contractile dysfunction, arrhythmias as well as irreversible myocytes damage. A total of 24 Swiss albino adult male mice were randomized into 4 equal groups. Group (1) sham group; Group (2) control group; Group (3) control vehicle (DMSO) group; Group (4) Ferulic acid treated group.Results of this study showed that levels of myocardial IL-6, were increased (p<0.001) in the control group. Histologically, all mice in control group showed significant cardiac injury (p<0.001). Ferulic acid significantly counteract the increase in myocardium level of IL-6 (P < 0.001). Histological analysis revealed that ferulic acid markedly reduced (P < 0.001) the severity of cardiac injury in the mice underwent LAD ligation procedure.Keywords: Ischemia-reperfusion (I/R) ,systemic inflammatory response syndrome (SIRS), multiple organ dysfunction syndrome (MODS) , Ferulic acid (FA), left anterior descending (LAD), Dimethylsulfoxide (DMSO).
Molecular diagnosis of Newcastle disease Iraqi Virulent strain virus HN gene by specific primers design
Authors: Al-Shammari Ahmed M. , Al-Nassrawei Huda A. , Murtadha A. M. H Murtadha A. M. H 
Published: August 2023

Newcastle disease is considered the most contagious poultry disease and may cause severe economic loss in the poultry industry, The first report in which NDV was consider as zoonotic disease and a human pathogen that was published by Burnet, in 1943. The aims of this study was to detect HN gene of two NDV Iraqi isolates (Najaf APMV1/ Chicken/ Iraq-najaf/ ICCMGR/2012and Baghdad ICCMGR) by designing specific priemrs for the Iraqi isolates. DNV isolates propagated in embryonated chicken eggs and showed to kill embryos in different times intervals ( more than 40 hours and less than 72 hours) with marked sever hemorrhage in infected embryos and it was similar in the two isolates, agglutination activity reflect titer (1024) for Najaf isolate and (512) for Baghdad isolate. The present study successfully detected part of (HN) gene by using specific primers designed for one-step RT-PCR undertaken to use them on molecular diagnosis of the hemagglutinin-neuraminidase (HN) gene in the Iraqi isolates and can be used for NDV virus detection in Iraq.
Etanercept ameliorate cardiac damage and Apoptosis Induced by Myocardial Ischemia/Reperfusion in male mice.
Authors: Hadi Najah R. , Yousif Fadhil G. , Yousif Maitham G , Hassan Saif M. 
Published: August 2023

Ischemia-reperfusion of cardiac tissues may lead to a prominent damage of the cardiomyocyte through either necrosis or apoptosis that seems to be the predominant modes of death during this period. In this study, we investigated the effects of Etanercept in regional ischemia/ reperfusion injury and apoptosis. White albino adult male mice were divided into four groups (six mice per group). Sham group, mice were subjected for all surgical procedure without ligation of the left interior descending coronary artery (LAD). Control group, in which LAD was ligated. Control vehicle and Etanercept treated groups, mice subjected to the surgical procedure with ligation of LAD for 30 minutes followed by 120 minutes reperfusion, mice treated with normal saline (etanercept vehicle) and Etanercept (5 mg/kg, I.P. 5 minute before reperfusion). At the end of reperfusion, cardiac tissue caspase 3 and Bcl-2, as well as plasma cardiac troponin I (cTnI) were measured. It has been found that Etanercept treated group showed significant reduction (P˂0.01) in caspase 3and cTnI but increase (P˂0.01) the level of the Bcl-2 as compare with the control groups. Histopathology study revealed that the treatment with Etanercept significantly (P˂0.01) improved cardiac injury as compared with control groups and the total severity scores showed showed 16.7 % of the group had no damage and 50% had mild cardiac injury and 33.3% had moderate cardiac injury of Etanercept treated group. It is concluded that Etanercept reduces cardiac damage and apoptosis associated with ischemia/reperfusion injury.
Effect of probiotics “Vetlactoflorum-M” and “Vetlactoflorum-C” on some serum blood biochemical parameters of broiler chickens
Authors: Al-Aqaby AamerRassam Ali , Glaskovich A.A. 
Published: August 2023

The aims of this study were to evaluate effect of using liquid preparation "Vetlactoflorum-M" (dissolved in diluted milk) and "Vetlactoflorum-C" (dissolved in whey) on some parameters such as concentration of cholesterol, triglyceride, glucose and uric acidin blood of broiler chickens. One hundred fifty broilers chicken breed "Ross-308" used in one day old, divided into three groups (each of 50 chicks as follow: (control" un supplemented probiotic" , first treatment group received “Vetlactoflorum-C” and second treatment group received “Vetlactoflorum-M”). Blood samples were taken in 7, 14, 21, 28, 35 and 42 day-old. The results showed that the selected probiotics ("Vetlactoflorum-M" and "Vetlactoflorum-C") are able to reduce uric acid, increase glucose. Lower concentrations of serum cholesterol and triglycerides were observed in the treatment groups. So the results were improved by using probiotic.
Determination of some biochemical parameters in clinically healthy and anemic goats
Authors: AL-Dujaily A.H. , AL-Hadithy H. AH 
Published: August 2023

The study was conducted on 130 healthy and 72 affected with iron deficiency anemia (IDA) by used local breed goats to determine serum iron, total iron binding capacity (TIBC), unbound iron binding capacity (UIBC), copper concentration and transferrin saturation (TS%). The 130 normal goats (40 males and 90 females). while 72 diagnosed iron deficient anemic goats (18males and 54females), both groups aged 6 months- 4 years in Najaf governorate. Blood samples were collected from the jugular veins into plain tubes during October 2013 till February2014, and the separated sera were directly used for chemical investigation. Result showed in normal and iron deficient anemic goats; Serum iron concentration was 7-17.9 and 11.8±0.22, 1.2-6.9 and 4.3±0.17 respectively, TIBC 67.5-107.3 and 89.7±0.69, 67.3-265 and 112.4±4.35 respectively ,UIBC 59.1-94.9 and 78±0.69, 60.9-260 and 108.1±4.36 respectively, TS% 7.4-20 and 13.1±0.25, 0.90-10.1 and 4.1±0.23 respectively and serum copper 1.1-4.6 and 2.4±0.05, 0.7-3.0 and 1.7±0.06 respectively.However, There were a significant (P<0.05) decrease in serum iron, TS% and copper, while there was a significant increase (P<0.05) in serum TIBC and UIBC of IDA goats as compared with normal control. Also, a significant differences(P<0.05) between males and females, as well as, in subgroups of normal and anemic goats have been recorded . In conclusion the present data recorded reference ranges of some biochemical parameters in local breed goats with a significant differences between normal, anemic ,males and females, and in different physiologic status .
Serosurvillance on Toxoplasmosis in Camels (Camelusdromedarius) at Al-Najaf Province
Authors: Mahmoud , Marai H. S , Al-Rubaie ,Abdel-Elah S.M , Al-Jeburii , Kefah O. S , Taha ,  Abdel-Kareem A. 
Published: August 2023

The current study was conducted to evaluate the seroprevalence of toxoplasmosis in camels resident in Al-Najaf province in Iraq . Serum samples were collected from 360 camels, 219(60.83%) of samples represents males and 141(39.16%) were collected from females. Latex Agglutination Test(LAT) was applied to screen all serum samples for Toxoplasmosis while ELISA was also used to confirm the positive result obtained by LAT Using LAT, out of 360 serum samples 91 (25.2% ) were positive to Toxoplasmagondii. Percentage of positive cases was more in females (30.4 %) than it is in males (21.9 %); the percent of seropositivity increased by increasing animals age. The ELISA test showed that 15 (16.4 %) samples were positive for toxoplasmosis, the males were 7 (14.5 %)which was lower than the percentage in females 8 (18.6 %). Also the percentage of positivity increased with increasing animals age.
The influence of chitosan on immune status and survival rate of Cyprinus carpioL. challenged with Aeromonas hydrophila
Authors: Mustafa Sanaa A. , Alfaragi Jamal K. , Aref Zeyad 
Published: August 2023

The aim of our study was to evalute the effect of chitosan on non- specific immunity and survival rate of Cyprinus carpio challenged with Aeromonas hydrophila. A total of 80 common carp (36.4 ± 0.73 g) were devided into four dietary groups: The first group (G1) was served as control fed on chitosn-free diet, the second group (G2) was fed on diet supplemented with 0.75% chitosan, the third group (G3) was fed on diet supplemented with 1.5% chitosan and the fourth group (G4) was fed on diet supplemented with 2% chitosan for 45 days. After 45 day of feeding trail, 6 fish per treatment were sampled for immunity determination (respirotary burst activity, bacteriocidal actvity and lysozyme arctivity). 36 fish in all treatment groups were challenged intramusculary with Aeromonas hydrophila on day 45. The results indicated that the fish fed on diet containing 2% chitosan (G4) had the significant increased (P<0.05) in respirotary burst activity, bacteriocidal actvity and lysozyme arctivity compared to control group and to other treatment groups, followed by G3 and G2 respectively. Also, G4 showed highest resistance to challenged A. hydrophila compared to other groups. Control group (G1) showed the decreased performance in all non sepecific immune parameters and simultaneously increased in mortality rate. Therefore, these results indicate that 2% chitosan as addititive fed could be used as prophylactic in common carp culture to enhance the protection against any possible infection by A. hydrophila which is useful for practical fish culture.
Isolation and identification of vibrio fluvailis from goldfish Carassiusauratus In AL Najaf Al-Ashraf Governorate
Authors: AL-Darwesh Ali Adnan 
Published: August 2023

This study aimed to diagnose the Vibrio fluvailis and identify some of the virulence genes, Eighty six samples were obtained from gold fishCarassiusauratus which were suffering from infection. Fourty one samples of gold fishCarassiusauratus gave a positive result for the Vibrio fluvailis, in percentage ( 47.6%) . The biochemical tests were conducted to confirm the infection as well as the examination of API 20. Polymerase chain technique was used to identify virulence genes hupO that have a role in the pathogenesis of bacteria isolated.
Effect of vildagliptin on atherosclerosis progression in high cholesterol –fed male rabbits
Authors: Majeed Sahar A 
Published: August 2023

Back ground: Atherosclerosis is a disease of large and medium-sized muscular arteries and is a progressive disease characterized by the accumulation of lipids and fibrous elements in the large arteries.Objective: This study was undertaken to assess the effect of vildagliptin on atherosclerosis via interfering with inflammatory and oxidative pathwaysMaterials and Methods: 18 local domestic male rabbits were included in this study. The animals were randomly divided into three groups (6rabbits in each group): Group I rabbits fed normal chow (oxiod) diet for 12 weeks. Group II rabbits fed 1% cholesterol enriched diet for 12 weeks. Group III rabbits fed with cholesterol enriched diet for 6 weeks, and then continued on cholesterol enriched diet and treated with vildaglipin 50mg/kg/day orally for the next 6 weeks. Blood samples were collected at the start of the study, at 6weeks of the study and then at the end of treatment course to measure Serum lipids profile [(TC), (TG), (HDL)] , hsCRP and TNFα. At the end of the study the aorta were removed for measurement of aortic MDA ,glutathione, sectioning for histopathology and measuring aortic intima- media thicknessResults: Treatment of rabbits with vildagliptin for 6 weeks results in a significant reduction (P<0.05) in serum level of TC, TG, hsCRP and TNFα and a significant increase (P<0.05) in serum HDL level. There was a significant reduction (P<0.05) in aortic MDA and intima-media thickness, in comparison to the rabbits in the induced untreated control group. vildagliptin treatment cause significant increment (P<0.05) in aortic GSH in comparison to induced untreated group .Regarding histopathological results, vildagliptin treatment for 6 weeks results in a significant reduction (P<0.05) in atherosclerotic lesions in comparison to the induced untreated group and significant reduction in aortic intima- media thickness(P<0.05)Conclusions : vildagliptin reduced atherosclerosis progression in hyperlipidemic rabbit via its effect on lipid parameters and interfering with inflammatory and oxidative stress pathway.
The hematological parameters in clinically normal lactating and ewes affected with mastitis
Authors: AL- Hadithy Harith Abdul-Hadi , Suleiman Jassim Mohamed 
Published: August 2023

The aim of the present study was to the determine the ranges and means ± SE of hematological parameters in Naemi clinically healthy lactating and ewes with mastitis . The study was conducted on 50 normal and 50 ovine mastitis ( 19 clinical and 31 subclinical ) infected with staphylococcus aureus, both groups aged 2-5 years in Salah al-ddin governorate / Iraq . The blood samples were collected from jugular vein during the period October 2012 till April 2013 . Results showed that the means ± SEin normal lactating and ovine mastitis were as follows ; Packed cell volume ( PCV ) 28 ± 0.27 % and 21.76 ± 0.46 % , hemoglobin (Hb) 9.4 ± 0.12g /dl and 7.56 ± 0.14 g / dl , red blood cell count ( RBC ) 9.52 ± 0.11 ×10⁶/µL and 7.67±0.19 × 10⁶/µL , mean cell volume ( MCV ) 29.39 ± 0.19 fl and 28.43 ± 0.32 fl , mean cell hemoglobin ( MCH ) 9.83 ± 0.08 pg and 9.88 ± 0.08 pg , mean cell hemoglobin concentration ( MCHC ) 33.51 ± 0.19 g/dl and 34.94 ± 0.33 g/dl , erythrocyte sedimentation rate ( ESR ) 6.28 ± 0.15 mm / 24 hrs and 27.08 ± 2.97 mm / 24 hrs , Platelets 247.22 ± 17.88 × 10³/µL and 185.58 ± 14.55 ×10³/µL , white blood cell count ( WBC ) 8.18 ± 0.22 x10³/µL and 14.38±0.26 ×10³/µL, lymphocytes 4509.6 ± 127.6 and 5033.4 ± 412.7 / µL , neutrophils 2857.2 ± 129.2 and 7714.8 ± 415.5/µL , monocytes 371.3 ± 32.7 and 846.9 ± 38.8/µL , eosinophils 331.2 ± 18.8 and 294.7 ± 24.4/µL and basophils 100.6 ± 7.3 and 463 ± 33 / µL respectively . The PCV, Hb , RBC and platelets were significantly higher ( P < 0.05) in normal lactating compared to that of ovine mastitis . The MCV, MCH and MCHC showed no significant differences between normal and ewes with matitis. While ESR and WBC count were significantly lower in normal lactating. Moreover the results revealed significant differences (P < 0.05) in some hematological values of different subgroups .
Isolation EnterohemorrhagicEscherichia coli from gall bladder of buffaloes and determine its sensitivity for antibiotics
Authors: Najum Ali Anok , Esmaeel Jameela Radi , Mizher Balsam Miri 
Published: August 2023

Enterohemorrhagic Escherichia coli (EHEC) has been associated with hemorrhagic colitis, a severe form of diarrhea, and with hemolytic uremic syndrome, This study was preformed to find EHEC in gall bladder of buffaloes , (150 gall bladder) samples were collected from slaughter house and cultured on MacConky agar then identified by culture on CefiximTellurite Sorbitol-MacConkey, agar then tested with several types of antibiotics and serotyping of isolates to determine EHEC, in addition to conjucation methods were preformed .Results showed that 13 isolates of E.coli were obtained and 6 strainS were grown on CefiximTellurite Sorbitol-MacConkey agar and positive for specific antisera of (EHEC O157:H7), and all strains were only sensitive for nitrofurane, When conjucation done all recipent cell were resistant for antibiotics: (ampicillin,streptomycin,sulfonamide,trimthoprine,tetracycline,chloramphenicol,gentamycin and amikacin).
Molecular detection of Hydatid cyst isolated from Goats in AL-Qadisiyia province by Polymerase Chain Reaction
Authors: Alkhaled Mansoor Jadaan Ali 
Published: August 2023

The present study was conducted during the period from August 2013 to April 2014 in AL-Qadisiyia province to detect the infection of hydatidiosis in Goat using Molecular and conventional methods. One hundred and seventy nine Goat were examined at slaughters in Aldewanyia abattoir. Caprinehydatid cyst were removed from infected liver and lung after visceral inspection of these organs then investigated the viability of protoscolices by staining with eosin stain then examining under light microscope of 40x.The results showed 45 hydatid cyst were found from 179 goat , the prevalence rate of infection (25.13%) . 20 cyst were found fertile only whereas 25 cyst were sterile. Regarding to infection site in organs, out of 45 hydatid cyst collected , 28(62.22%) cyst were isolated from liver whereas 17 (37.77%) isolated from lung with significant differences at p<0.05. Out of 45 hydatid cyst samples were taken from infected goats, and examined by conventional PCR , the PCR product demonstrated an expected fragment of 440 bp in the length , 10samples were given positive cases , 7(25%) samples from liver whereas only 3(12%) from lung samples gave positive PCR result, with statistically significant difference (P<0.05) between two organs.