bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary modulation of epigenetics: Implications for cancer prevention and progression
Authors: Zhu Si-Rui , Liang Xin-Yu , Bai Qian , Ni Xiang-Min , Wang Jian 
Published: January 2025

Abstract
Epidemiological studies indicate a strong correlation between various types of human cancer and dietary factors, whereas the specific mechanisms remain to be fully elucidated. Epigenetic alterations, such as DNA methylation, histone modifications, and noncoding RNA, are influenced by dietary components, especially phytochemicals and nutrients that participate in one-carbon metabolism. These alterations significantly impact cancer occurrence and progression. Consequently, epigenetic pathways may mediate the effects of diet on cancer risk. This review synthesizes the current information regarding the association of epigenetic alterations with cancer initiation and development, as well as the mechanisms by which diet exerts its influence on these changes. The goal of this minireview is to enhance the understanding of the roles of diet on epigenetic alterations to improve cancer prevention and treatment through diet.
The model of fear of movement/(re)injury runs clockwise from catastrophizing: evidence from a sample of outpatients with chronic non-specific low back pain
Authors: Monticone Marco , Arippa Federico , Frigau Luca , Foti Calogero , Ferrari Silvano , Guicciardi Marco , Rocca Barbara 
Published: January 2025

ABSTRACT

Objective
To provide evidence that catastrophizing is the primer of the cognitive-behavioural model of fear of movement/(re)injury (FAM).

Design
A cross-sectional analysis of 180 outpatients with chronic non-specific low back pain who completed the Pain Catastrophizing Scale (PCS), the Tampa Scale of Kinesiophobia (TSK), the Roland-Morris Disability Questionnaire (RMDQ), the Hospital Anxiety and Depression Scale – Depression (HADS-D), and a pain intensity numerical rating scale (NRS). The intercorrelations of the outcome measures were estimated using Pearson’s correlation coefficient (r), and regression analyses were used to examine their predictive values by following the left side of the FAM clockwise from the PCS (p = 0.05). A postdictive analysis dichotomizing the sample into high- and low-level catastrophizers evaluated the effects of addressing catastrophizing on disability and pain.

Results:
The intercorrelations of the PCS with the TSK, RMDQ, HADS-D and NRS were respectively r = 0.59, r = 0.54, r = 0.18, and r = 0.44. PCS was a significant predictor of TSK; PCS and TSK significantly predicted RMDQ and HADS-D; and PCS, TSK, RMDQ and HADS-D significantly predicted NRS. The postdictive analysis showed that addressing catastrophizing reduces disability and pain experience by 14% in high-level catastrophizers and 86% in low-level catastrophizers.

Conclusion
Our findings provide evidence that catastrophizing is the primer of the FAM.
ADAMTS4-Specific MR Peptide Probe for the Assessment of Atherosclerotic Plaque Burden in a Mouse Model
Authors: Mangarova Dilyana B. , Kaufmann Jan O. , Brangsch Julia , Kader Avan , Möckel Jana , Heyl Jennifer L. , Verlemann Christine , Adams Lisa C. , Ludwig Antje , Reimann Carolin , Poller Wolfram C. , Niehaus Peter , Karst Uwe , Taupitz Matthias , Hamm Bernd , Weller Michael G. , Makowski Marcus R. 
Published: January 2025

Introduction
Atherosclerosis is the underlying cause of multiple cardiovascular pathologies. The present-day clinical imaging modalities do not offer sufficient information on plaque composition or rupture risk. A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) is a strongly upregulated proteoglycan-cleaving enzyme that is specific to cardiovascular diseases, inter alia, atherosclerosis.

Materials and Methods
Male apolipoprotein E-deficient mice received a high-fat diet for 2 (n = 11) or 4 months (n = 11). Additionally, a group (n = 11) receiving pravastatin by drinking water for 4 months alongside the high-fat diet was examined. The control group (n = 10) consisted of C57BL/6J mice on standard chow. Molecular magnetic resonance imaging was performed prior to and after administration of the gadolinium (Gd)-based ADAMTS4-specific probe, followed by ex vivo analyses of the aortic arch, brachiocephalic arteries, and carotid arteries. A P value <0.05 was considered to indicate a statistically significant difference.

Results
With advancing atherosclerosis, a significant increase in the contrast-to-noise ratio was measured after intravenous application of the probe (mean precontrast = 2.25; mean postcontrast = 11.47, P < 0.001 in the 4-month group). The pravastatin group presented decreased ADAMTS4 expression. A strong correlation between ADAMTS4 content measured via immunofluorescence staining and an increase in the contrast-to-noise ratio was detected (R
2 = 0.69). Microdissection analysis revealed that ADAMTS4 gene expression in the plaque area was significantly greater than that in the arterial wall of a control mouse (P < 0.001). Laser ablation–inductively coupled plasma–mass spectrometry confirmed strong colocalization of areas positive for ADAMTS4 and Gd.

Conclusions
Magnetic resonance imaging using an ADAMTS4-specific agent is a promising method for characterizing atherosclerotic plaques and could improve plaque assessment in the diagnosis and treatment of atherosclerosis.
Virtual Monitoring Technician Performance in High-Fidelity Simulations of Remote Patient Monitoring
Authors: Sanghavi Harsh , Peng Yuhao , Tetteh Emmanuel , Parker Sarah Henrickson , Wolf Laurie D. 
Published: January 2025

Introduction
Virtual Monitor Technicians (VMTs) are crucial in remotely monitoring inpatient telemetry. However, little is known about VMT workload and intratask performance changes, and their potential impact on patient safety. This exploratory study used a high-fidelity simulation aimed to evaluate VMTs' workload and performance changes over time in telemetry monitoring and identify future research directions for performance improvement.

Methods
The research team created a simulation of the current remote telemetry stations with 36 patient waveforms across 3 screens alongside a documentation screen, replicating VMTs' work. Twelve VMTs participated in a 1-hour session, and time-to-escalate and detection accuracy to auditory/visual alerts were recorded. Workload was measured using the NASA-Task Load Index.

Results
The post-task NASA-Task Load Index score showed an increased workload score of 64 of 100 from a prescore of 38 of 100, with mental and temporal demands being the largest contributors. The performance of VMTs did not change significantly over time, with a 52% correct response rate. Participants' ability to detect signals was slightly better than chance (d′ = 0.477), and they tended to be cautious in their responses, β (M = 1.989, SD = 1.635). Urgent, Warning, and Medium audiovisual alerts were recognized in 9, 35, and 39 seconds, respectively, whereas advisory alerts (visual only) were recognized in 13 minutes.

Conclusion
This study sets a foundation for future work on VMT workload expectations. Although our work is exploratory, the results indicate a significant increase in VMT workload with no decline in performance; VMTs responded most quickly and accurately to urgent alerts, whereas overall response accuracy to nonurgent alerts was marginally better than chance. Future research needs to explore techniques to improve response accuracy rate beyond the 52% measured in this study.
Evaluating Delta-8-THC–Induced Psychosis: A Systematic Review
Authors: Ralston Megan Jayne , Osman Alim 
Published: January 2025

Objectives
This study reviews literature on the psychiatric effects of delta-8-THC, particularly psychosis and severe mental health outcomes, to highlight the need for further research and regulation.

Background
Marijuana, the most widely used illicit drug in the United States, sees increasing use due to legalization. Although moderate use is generally safe, adverse effects can occur, especially in those with preexisting conditions. Delta-9-THC is known for its psychoactive effects and potential to induce psychosis. Delta-8-THC, another cannabinoid, is gaining popularity and has been linked to severe adverse events but remains under-researched.

Methods
A comprehensive search of PubMed and Web of Science followed PRISMA guidelines to identify studies and case reports on delta-8-THC and psychosis. Articles on delta-9-THC or other cannabinoids were excluded. Relevant studies were screened, and duplicates removed. The included studies were evaluated using the Critical Appraisal Skills Programme Checklist for Case Reports.

Results
The search identified 201 studies, with 12 meeting the inclusion criteria for full-text analysis. Six case reports, involving 9 patients, were reviewed. Most patients were male and in their 20s, with varied psychiatric histories, including no prior psychiatric history, schizophrenia, posttraumatic stress disorder, and generalized anxiety disorder. Reported symptoms included psychosis, mood lability, and cannabinoid hyperemesis syndrome. Treatments varied, with different clinical outcomes.

Conclusions
Delta-8-THC poses significant psychiatric risks despite being less intoxicating than delta-9-THC. The lack of Food and Drug Administration regulation and the availability of delta-8-THC products heighten these risks. More rigorous studies are needed to understand delta-8-THC's impact on mental health and inform regulatory actions.
Harmonizing the inner orchestra: the impact of urbanization and evolution of stress, inflammation, diet, and lifestyles in depression
Authors: Su Kuan-Pin 
Published: January 2025

Purpose of review
This review explores the intersection of urbanization, stress, inflammation, diet, lifestyle changes, and digital evolution as key factors influencing depression, emphasizing the necessity for integrative and evolutionary perspectives in mental health and intervention.

Recent findings
Epidemiological studies reveal a dramatic increase in common mental disorders, particularly depression, alongside parallel increases in suicide, unemployment, and divorce rates. Evolutionary perspectives suggest that mechanisms once advantageous for survival, such as stress responses and inflammatory processes, may contribute to mental health challenges in modern urban environments. Moreover, the mental health crisis among generation Z underscores the impact of digital evolution, where the shift from play-based to phone-based childhood contributes significantly to rising depression rates. Recent research highlights the significant role of omega-3 fatty acids, circadian rhythm regulation, mindfulness, and anti-inflammatory lifestyle interventions in mitigating depressive symptoms.

Summary
The interplay between our evolutionary heritage and rapid societal changes has created a “symphony” of factors contributing to depression. Understanding these evolutionary mismatches in stress responses, inflammatory processes, dietary shifts, circadian disruptions, and digitalization provides new insights into the etiology of depression. This holistic approach, conceptualized as “harmonizing the inner orchestra,” offers promising avenues for prevention and treatment strategies that address the multifaceted nature of depression in our modern world.
Warming increases richness and shapes assemblages of eukaryotic parasitic plankton
Authors: Rajarajan Amruta , Cerbin Sławek , Beng Kingsly C. , Monaghan Michael T. , Wolinska Justyna 
Published: January 2025

AbstractAnthropogenic activities have led to a global rise in water temperatures, leading to an increased interest in how warming affects infectious disease ecology. While most studies have focused on individual host-parasite systems, there is a gap in understanding the impact of warming on multi-host, multi-parasite assemblages in natural ecosystems. To address this gap, we examined freshwater eukaryotic parasite communities in ten natural lakes near Konin, Poland: five artificially heated and five nearby non-heated “control” lakes. Since 1958, the heated lakes have experienced a mean annual temperature increase of 2°C due to hot water discharge from two adjacent power plants. We collected seasonal environmental DNA (eDNA) samples from surface waters over a two-year period and applied targeted metabarcoding to compare the richness and distribution of eukaryotic parasites across lake types, with a focus on protists and fungi. Using literature searches and sequence metadata from GenBank, we identified putative parasites which included Alveolates, Stramenopiles, basal Fungi and Ichthyosporeans, as well as their associated hosts. Heated lakes exhibited distinct parasite assemblages, with higher richness of parasitic chytrids and aphelids particularly during summer, suggesting thermal preferences among some freshwater parasite species. A general linear model suggests that increased chytrid richness in heated lakes may be partly due to higher richness of their green algal, diatom- and dinoflagellate hosts. These findings highlight how warming can drive shifts in parasite richness and assemblages, potentially altering host-parasite dynamics in freshwater ecosystems.
Statistical Analysis Plan for the manuscript reporting the primary outcome in the TESSA study
Authors: Grunnet Louise Groth , Quist Jonas Salling , Færch Kristine , Blond Martin Bæk , Kengne Andre , Dave Joel , Goedecke Julia , Mendham Amy 
Published: January 2025

AbstractThis paper describes the statistical analyses plan (SAP) for the following study: Exploring the effects of time-restrictedeating on body weight and associated cardiometabolic outcomes inSouthAfrican women living with HIV (TESSA): a protocol for a randomised controlled trial.This document is a supplement to the TESSA study protocol and contains the statistical analysis plan (SAP) for the main paper of the trial in which the primary outcome will be reported. This document complies with the guidelines for the content of statistical analysis plans in clinical trials. The SAP does not cover the statistical analysis of data for outcomes to be included in secondary manuscripts.
Unveiling BPDE Induced Carcinogenic Signaling: Computational Insights into NF-κB, MAPK, and PI3K/Akt Pathway Activation
Authors: Kathpalia Karishma Vivek , Kumar Anil , Verma Awadhesh Kumar 
Published: January 2025

AbstractThe Carcinogen benzo[a]pyran-7, 8 dihydrodiol 9, 10 epoxide(BaP-DNP) the active metabolite of benzo[a]pyrene has been postulated to induce carcinogenesis through formation of adduct with DNA and through alteration of critical pathways involved in signaling. Besides genotoxicity, BPDE has more recently been reported to alter the NF-κB, MAPK, and PI3K/Akt, that are involved in inflammation, cell survival and cell proliferation. From the above molecular docking analysis of BPDE binding to the potential proteins of these pathways, the mechanism of action of the compound is examined. The studies conducted on docking reveal moderate binding affinity of BPDE with NF-κB, that is, -6.11 kcal/mol, which points out its role in inflammation and oncogenic signaling. In addition, BPDE has more protein-binding affinities within the MAPK and PI3K/Akt pathway for CDK1 (-7.46 kcal/mol), LOX (-7.47 kcal/mol), and CDK6 (-6.84 kcal/mol). These indicate that influence of BPDE in tumour progression should not be only through the activation of NF-κB but would rather be supported by significant inputs of pathways that include PI3K/Akt and MAPK. This interaction would seem to favor cell survival, proliferation, and apoptosis resistance. Broader analysis of the data presented allows defining NF-κB as one of the key molecules contributing to BPDE-induced carcinogenesis; however, the data also point to the significant participation of the MAPK and PI3K/Akt pathways in this process. Future work should also more fully detail how these pathways interconnect and also evaluate the effectiveness of inhibiting NF-κB and related pathways in preventing BPDE’s carcinogenic action. This study adds to understanding of molecular mechanisms and therapeutic targets of BPDE.Graphical Abstract
Establishing a preclinical chronic wound model to characterise the pathophysiology of snakebite envenoming
Authors: Dawson Charlotte A , Harijanto Pruistinne , Nawilaijaroen Yonlada , Casewell Nicholas R. , Cash Jenna L 
Published: January 2025

SummarySnakebite claims 138,000 lives a year with an additional 400,000 patients left permanently disabled or disfigured1. Morbidity following envenoming includes the development of chronic wounds around the bite site. The understanding of the underlying pathophysiology of chronic snakebite wounds has been severely limited by the historical reliance on a preclinical model that only captures acute local envenoming pathology. Through the application of three medically important snake venoms (Echis ocellatus, Bothrops atrox and Naja nigricollis) to a recently developed preclinical model of chronic wounds, we have been able to characterise key features of venom wounds. We have been able to show that venom wounds share consistencies with non-venom induced preclinical wounds, and also display unique characteristics such as extracellular matrix degradation and eosinophilic infiltrate. This model will not only serve to increase our understanding the underlying pathophysiology of venom wounds, but will also provide a platform for exploring therapeutic interventions to reduce or resolve snakebite wounds.
IMPORTANT: Advanced Pollen Classification of Indian Medicinal Plants through SEM and Computer Vision
Authors: Khalane Jaidev Sanjay , Gawande Nilesh D. , Raman Shanmuganathan , Sankaranarayanan Subramanian 
Published: January 2025

AbstractPollen grains of plant species have unique morphological characteristics. The variability in shape, size, and microscopic pollen surface features can be efficiently used to determine the plant species to which they belong. This approach can be instrumental in regions with rich biodiversity in plant species, specifically in medicinal plant. The creation of a pollen dataset for these species using SEM images and a computer vision application can be beneficial for their identification. We have developed a robust approach utilizing scanning electron microscopy (SEM) to generate high-resolution pollen images from 28 plant species of medicinal importance and employed computer vision techniques for accurate segmentation and classification based on diverse morphological features. In this study, a dataset comprising 269 images for segmentation and 5842 images was used for classification across 28 classes. In addition, we have created a globally accessible database named IMPORTANT (Indian Medicinal Plants Pollen Images Dataset for Research, Training, and Analysis of Neural Networks) to facilitate easy retrieval and sharing of pollen images. This study can effectively identify medicinal plant species by utilizing the microscopic features of pollen SEM images through the IMPORTANT database. Since the pollen of even closely related species vary in their morphological characteristics, this approach can efficiently determine the closely related species with accuracy and precision.
Endogenous falcarinol-type polyacetylenes in carrots and their putative influence on post-harvest fungal pathogensMycocentrospora acerinaandBotrytis cinerea
Authors: He Wanying , Dunemann Frank 
Published: January 2025

AbstractFalcarinol-type polyacetylenes are a main group of natural bitter compounds synthesized in carrot taproots and putatively contribute to defence mechanisms against soil-borne fungal pathogens. In this study, we analysed the relationships between the constitutive levels of these secondary metabolites and the extent of root infection with the two main carrot storage fungal pathogensMycocentrospora acerina(liquorice rot) andBotrytis cinerea(grey mold). Taproots of eight differently colored carrot cultivars exhibiting different levels of the three main falcarinol-type polyacetylenes were inoculated with the two fungi and evaluated for diseased area with a digital image analysis system after 6-weeks incubation in a cold storage facility. After inoculation on cortex tissue and the periderm of taproots, a progressed carrot breeding line and the cultivar Bolero demonstrated the highest tolerance to bothM. acerinaandB. cinerea, while Blanche and Senta were the most susceptible cultivars. Concentrations of falcarinol-type polyacetylenes were negatively correlated, though not significantly, withM. acerinaandB. cinereadisease severity. Nevertheless, our study has provided further insights into the naturally occurring variability for the accumulation of falcarinol-type polyacetylenes in cultivated carrots and their putative contributions to resistance against post-harvest fungal pathogensM. acerinaandB. cinerea. To our knowledge, this is the first study about the pathogenicity ofB. cinereaafter inoculation of different carrot cultivars with known root PA levels.
Vascular contribution to cognitive impairment in heart failure with preserved ejection fraction: TRPV4 and KLF2 as key mediators of neurovascular dysfunction in the ZSF1 model
Authors: Lambrichts Sara M.P. , van der Taelen Laura , Pastor Irene , Leenders Peter , Bitsch Nicole , Majcher Daria , Hermes Denise , Simmonds Steven J. , van Herwijnen Marcel , Kamps Rick , Weltjens Ellen , Steinbusch Hellen P. , Caiment Florian , Kutmon Martina , Jones Elizabeth A.V. , van Oostenbrugge Robert J. , Foulquier Sebastien 
Published: January 2025

ABSTRACTBackgroundThe development of vascular cognitive impairment (VCI) and heart failure with preserved ejection fraction (HFpEF) are strongly associated with comorbidities such as obesity, diabetes, hypertension, and aging. Microvascular dysfunction may be key a pathological step in the development of cognitive dysfunction during HFpEF. Hence, we aimed to evaluate the cerebrovascular and cognitive phenotype in ZSF1 rats and identify molecular processes central to the development of VCI during HFpEF.MethodsMale Lean and Obese rats underwent blood pressure and glucose measurements, echocardiography and a series of behavioural tasks at three different time points. Cerebral blood flow was measured over the barrel cortex using laser speckle contrast imaging and neurovascular coupling was assessed upon whisker stimulation. Brain immunohistochemistry was performed to assess blood- brain barrier (BBB) integrity and vascular density. Lastly, isolated cortical microvessels were used for transcriptomic analysis, and selected targets were validated in brain sections via fluorescent multiplex in-situ hybridization.ResultsObese ZSF1 rats exhibited neurovascular uncoupling, along with an impaired short- and long- term memory, as well as spatial learning. In addition, BBB permeability and cerebral vascular density were elevated in Obese vs Lean at 22-23 and 34-35 weeks of age, respectively. Transcriptomic analysis of brain microvessels revealed the regulation of processes related to angiogenesis, vasoreactivity, immune mechanisms and vascular remodelling. Among the top regulated biological processes,Trpv4andKlf2were found to be consistently downregulated in Obese vs Lean rats and involved in many of the top regulated biological processes. This was further verified in brain sections at 22-23 weeks of age.ConclusionObese ZSF1 rats develop cognitive impairment, which is related to dysfunction of the neurovascular unit. This cerebrovascular phenotype progresses along with the onset of HFpEF and is associated with downregulation ofTrpv4andKlf2in cerebral microvessels, two key genes known for their vasoprotective actions.