bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reproducing in hot water: Experimental heatwaves deteriorate multiple reproductive traits in a freshwater ectotherm
Authors: Breedveld Merel C. , Devigili Alessandro , Borgheresi Oliviero , Gasparini Clelia 
Published: January 2023

Abstract

Heatwaves are occurring at an increasing frequency and intensity under ongoing climate change. As many reproductive traits—including mating behaviour and gamete traits—are sensitive even to small temperature changes, the impact of heatwaves on reproduction and sexual selection processes is likely to be vast. Also, evaluating whether the sexes respond differently to these extreme events is crucial to understand the impact on fecundity and the consequences at the population level. Nonetheless, our knowledge of the effects of heatwaves on these key aspects of animal life is still limited.

Here, we expose recently mated male and female guppies Poecilia reticulata to an experimental heatwave (32°C, 6°C above the control, for 5 days) to determine its effects on several traits, including sexual behaviour, condition, ornamentation and fertility. Using this design, in contrast to alternative experimental setups, we had the possibility to attribute the effects of the heatwave to males' and females' reproductive traits independently.

Overall, our results indicate that heatwaves can drastically affect key reproductive traits and unravel sex‐specific responses. In males, there was no effect of the heatwave on survival, but both pre‐ and postcopulatory reproductive traits were affected. After the heatwave, we detected a decrease in orange colouration (the most important ornament on which female choice is based) and the overall level of sexual activity, and a shift in the preferred mating tactic towards forced copulation attempts. The latter suggests implications in sexual conflict dynamics, as forced copulations override female mate choice. Also, after the heatwave, males had more sperm but of lower quality, and, in addition, an increased variance in sperm number. Overall, heatwaves may thus result in a compromised ability to secure matings and fertilizations. In females, the heatwave significantly affected survival, with increased mortality in the short term, and impaired fecundity, with many females from the heatwave treatment not reproducing at all.

The negative effects of heatwaves on key reproductive traits unravelled by our study could have major implications for population dynamics and persistence. It highlights the need for further studies on how these extreme events affect reproduction, to improve our understanding of the impacts of climate change.

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Who Delivers Maternal and Child Health Services? The Contributions of Public Health and Other Community Partners
Authors: QUINLAN TARYN A. G. , MITCHELL AMELIA L. , MAYS GLEN P. 
Published: January 2023

Policy Points
Local health departments with direct maternal and child health service provisions exhibit greater social service collaboration, thereby enhancing community capacity to improve health care access and social determinant support.
These findings may prioritize collaboration as a community‐based effort to reduce disparities in maternal and child health and chronic disease.
ContextImproving maternal and child health (MCH) care in the United States requires solutions to address care access and the social determinants that contribute to health disparities. Direct service provision of MCH services by local health departments (LHDs) may substitute or complement public health services provided by other community organizations, impacting local service delivery capacity. We measured MCH service provision among LHDs and examined its association with patterns of social service collaboration among community partners.MethodsWe analyzed the 2018 National Longitudinal Survey of Public Health Systems and 2016 National Association of County and City Health Officials Profile data to measure the LHD provision of MCH services and the types of social services involved in the implementation of essential public health activities. We compared the extensive and intensive margins of social service collaboration among LHDs with any versus no MCH service provision. We then used latent class analysis (LCA) to classify collaboration and logistic regression to estimate community correlates of collaboration.FindingsOf 620 LHDs, 527 (85%) provided at least one of seven observed MCH services. The most common service was Special Supplemental Nutrition Program for Women, Infants, and Children (71%), and the least common was obstetric care (15%). LHDs with MCH service provision were significantly more likely to collaborate with all types of social service organizations. LCA identified two classes of LHDs: high (n = 257; 49%) and low (n = 270; 51%) collaborators. Between 74% and 96% of high collaborators were engaged with social service organizations that provided basic needs services, compared with 31%‐60% of low collaborators. Rurality and very high maternal vulnerability were significantly correlated with low collaboration among MCH service–providing LHDs.ConclusionsLHDs with direct MCH service provision exhibited greater social service collaboration. Collaboration was lowest in rural communities and communities with very high maternal vulnerability. Over half of MCH service–providing LHDs were classified as low collaborators, suggesting unrealized opportunities for social service engagement in these communities.
Study on structural characteristics and physicochemical properties of starches extracted from three varieties of kudzu root (Pueraria lobata starch)
Authors: Duan Xuantong , Guan Yongmei , Dong Huanhuan , Yang Mei , Chen Lihua , Zhang Hua , Naeem Abid , Zhu Weifeng 
Published: January 2023

AbstractKudzu root (Pueraria lobata) is well known for its traditional use as a medicinal food homologous plant in China. Three varieties of kudzu roots, such as Gange‐1, Gange‐2, and Gange‐6, are commonly used. Nowadays, kudzu starch (KS) is commercially available as satiating foods or product ingredients. Differentiation and selection of the variety are important components of quality control for KS‐based products. Thus, the present work was aimed at comparing the physicochemical properties, such as thermodynamic properties, pasting properties, solubility, swelling, as well as the structural characteristics of the starches extracted from the three varieties of kudzu roots. The results show that KS‐6 has a higher content of functional ingredients thus can be used as an ideal functional starch. However, KS‐6 has a higher amylopectin:amylose ratio of 4.65, resulting in a better solubility, higher transition temperature, and higher gelatinization enthalpy. KS‐2 showed lower transition temperature and gelatinization enthalpy, as well as higher peak viscosity, through viscosity, and final viscosity. KS‐1 could result in a soft texture after pasting. The appropriate variety of KS should be differentiated and selected according to application scenarios. This study provided valuable insights into the potential use of different KS in the food and nonfood industries.Practical Application1. KS‐1 was found to be suitable for use as a food supplement. 2. KS‐6 has the highest nutritional value. 3. They can be used as a substitute for other similar starches.
Insights into gene tissue specificity and protein–protein interactions in the context of purifying selection in humans
Authors: Mezzavilla Massimo , Cocca Massimiliano 
Published: January 2023

AbstractBackgroundHow much are natural selection and gene characteristics, such as the number of protein‐protein interactions (PPIs), tissue specificity (𝞽), and expression level, connected?MethodsIn order to investigate these relationships, we combined different metrics linked to genetic constraints and analyzed their distribution concerning PPIs, 𝞽 and expression levels.ResultsWe discovered a positive correlation between genetic constraints, PPIs, and expression levels in all tissues. On the other hand, we obtained a negative correlation between genetic constraints and 𝞽. Furthermore, the fraction of variance in PPI and 𝞽 explained by the constraints metrics is around 6% and 10%, respectively.ConclusionsWe observed that the variance of expression of tissue‐specific genes seems not related to their level of selection constraints, which is the opposite of what is found on non‐tissue‐specific genes. Overall these observations would help to elucidate the relationship between natural selection and gene features.
Multiple interfaces
Authors: Udupa Sahana , Kramer Max 
Published: January 2023

AbstractIn India and its diaspora in the UK, online activities of various sorts—tweeting, blogging, messaging, trolling, and tagging—have become central to tensions surrounding religion's presence in public life and the stakes of belonging to the nation. Three clusters of social media practices undergird these digital mediations: piety, surveillance, and fun. Such practices reveal how internet‐enabled mediations reenergize religion as a political category of difference under majoritarian right‐wing regimes and the transnational context of Islamophobia, while also offering distinct possibilities for imagining politics through the pleasures, visibilities, and reflections induced by digital circulations. Rather than approaching the internet as an abstract technological context or discrete channels for communication, this analysis theoretically positions it as an arena of “multiple interfaces.” It signals contiguities and collisions that digital practice has opened up among the very real communities and structures of authority, under conditions shaped by longer colonial histories.
Up‐regulation of oxytocin receptors on peripheral sensory neurons mediates analgesia in chemotherapy‐induced neuropathic pain
Authors: Li Lixuan , Li Pupu , Guo Jing , Wu Yifei , Zeng Qian , Li Nan , Huang Xiaoting , He Yongshen , Ai Wen , Sun Wuping , Liu Tao , Xiong Donglin , Xiao Lizu , Sun Yanyan , Zhou Qiming , Kuang Haixia , Wang Zilong , Jiang Changyu 
Published: January 2023

Background and PurposeChemotherapy‐induced neuropathic pain (CINP) currently has limited effective treatment. Although the roles of oxytocin (OXT) and the oxytocin receptor (OXTR) in central analgesia have been well documented, the expression and function of OXTR in the peripheral nervous system remain unclear. Here, we evaluated the peripheral antinociceptive profiles of OXTR in CINP.Experimental ApproachPaclitaxel (PTX) was used to establish CINP. Quantitative real‐time polymerase chain reaction (qRT‐PCR), in situ hybridization, and immunohistochemistry were used to observe OXTR expression in dorsal root ganglia (DRG). The antinociceptive effects of OXT were assessed by hot‐plate and von Frey tests. Whole‐cell patch clamp was performed to record sodium currents, excitability of DRG neurons, and excitatory synapse transmission.Key ResultsExpression of OXTR in DRG neurons was enhanced significantly after PTX treatment. Activation of OXTR exhibited antinociceptive effects, by decreasing the hyperexcitability of DRG neurons in PTX‐treated mice. Additionally, OXTR activation up‐regulated the phosphorylation of protein kinase C (pPKC) and, in turn, impaired voltage‐gated sodium currents, particularly the voltage‐gated sodium channel 1.7 (NaV1.7) current, that plays an indispensable role in PTX‐induced neuropathic pain. OXT suppressed excitatory transmission in the spinal dorsal horn as well as excitatory inputs from primary afferents in PTX‐treated mice.Conclusion and ImplicationsThe OXTR in small‐sized DRG neurons is up‐regulated in CINP and its activation relieved CINP by inhibiting the neural excitability by impairment of NaV1.7 currents via pPKC. Our results suggest that OXTR on peripheral sensory neurons is a potential therapeutic target to relieve CINP.
Overcoming the challenges associated with identification of deep intronic variants by whole genome sequencing
Authors: Dirix Marie , Gribouval Olivier , Arrondel Christelle , Benjelloun Saadia , Boyer Olivia , Charbit Marina , Antignac Corinne , Heidet Laurence , Dorval Guillaume 
Published: January 2023

AbstractWhole‐genome sequencing (WGS) now allows identification of multiple variants in non‐coding regions. The large number of variants identified by WGS however complicates their interpretation. Through identification of the first deep intronic variant in NPHS2, which encodes podocin, a protein implicated in autosomal recessive steroid resistant nephrotic syndrome (SRNS), we compare herein three different tools including a newly developed targeted NGS‐based RNA‐sequencing to explore the splicing effect of intronic variations.WGS identified two different variants in NPHS2 eventually involved in the disease. Through RT‐PCR, exon‐trapping Minigene assay and targeted RNA sequencing, we were able to identify the splicing defect in NPHS2 mRNA from patient kidney tissue. Only targeted RNA‐seq simultaneously analyzed the effect of multiple variants and offered the opportunity to quantify consequences on splicing. Identifying deep intronic variants and their role in disease is of utmost importance. Alternative splicing can be predicted by in silico tools but always requires confirmation through functional testing with RNA analysis from the implicated tissue remaining the gold standard. When several variants with potential effects on splicing are identified by WGS, a targeted RNA sequencing panel could be of great value.
Nomadic boat‐dwelling children of Southeast Asia: Discourses on theSama‐Bajauchildren and implications on decentring child migration studies
Authors: Andal Aireen Grace 
Published: January 2023

AbstractThis article examines the discursive narratives in academic literature about the nomadic boat‐dwelling children of the Sama‐Bajau group in Southeast Asia. Through examining academic literature from 1989 to 2021, this paper explores how the literature shapes and mediates the narratives about Sama‐Bajau children. Findings suggest two threads of discursive narratives—one takes a developmentalist lens, and the other offers alternative narratives that reveal the complex identities of Sama‐Bajau children. These observations highlight the importance of nuanced conversations on child sea nomadism towards further developments of critical childhood studies on child migration in Southeast Asia and beyond.
Latent classes and longitudinal patterns of material hardship as predictors of child well‐being
Authors: Thomas Margaret M. C. 
Published: January 2023

AbstractThis study examined the associations of multifaceted material hardship measured cross‐sectionally and longitudinally with children's well‐being in the United States. Results from linear regression and child fixed effects models indicated that more intense material hardship had consistent, detrimental associations with child health status and internalizing and externalizing behaviours. More intense longitudinal patterns of material hardship were consistently associated with behaviours only. These findings examine new, multifaceted measures of material hardship and suggest associations between child well‐being, particularly behaviour challenges, and exposure both to multiple forms of material hardship and to more intense long‐term patterns of hardship.
The assessment of cliques in bound child and adolescent groups: A systematic scoping review
Authors: Robertson Madison A. , Sutcliffe Jordan T. , McGuire Cailie S. , Bruner Mark W. , Côté Jean , Martin Luc J. 
Published: January 2023

AbstractThe purpose of this systematic scoping review was to examine research that has assessed cliques within bound child and adolescent groups. A total of 10,878 studies were obtained from six research databases, with 68 studies ultimately being included. Results highlighted a range of methods used for the assessment of cliques with social network analysis and peer nominations being most prevalent. Moreover, research question(s) were encompassed within the following categories: antecedent‐oriented (n = 13), outcome‐oriented (n = 43) or the general nature of cliques (n = 12). These findings demonstrate the complexity of cliques and the need for nuanced approaches to exploring their implications for youth.
Effects of forest fragmentation on avian breeding activity
Authors: Coddington Charles P. J. , Cooper W. Justin , Luther David A. 
Published: January 2023

AbstractBiodiversity declines and ecosystem decay follow forest fragmentation; initially, abundant species may become rare or be extirpated. Underlying mechanisms behind delayed extirpation of certain species following forest fragmentation are unknown. Species declines may be attributed to an inadequate number of breeding adults required to replace the population or decreased juvenile survival rate due to reduced recruitment or increased nest predation pressures. We used 10 years of avian banding data, 5 years before and 4 years after fragment isolation, from the Biological Dynamics of Forest Fragments Project, carried out near Manaus, Brazil, to investigate the breeding activity hypothesis that there is less breeding activity and fewer young after relative to before fragment isolation. We compared the capture rates of active breeding and young birds in 3 forest types (primary forest, fragment before isolation, and fragment after isolation) and the proportion of active breeding and young birds with all birds in each unique fragment type before and after isolation. We grouped all bird species by diet (insectivore or frugivore) and nesting strategy (open cup, cavity, or enclosed) to allow further comparisons among forest types. We found support for the breeding activity hypothesis in insectivorous and frugivorous birds (effect sizes 0.45 and 0.53, respectively) and in birds with open‐cup and enclosed nesting strategies (effect sizes 0.56 and 0.44, respectively) such that on average there were more breeding birds in fragments before isolation relative to after isolation. A larger proportion of birds in the community were actively breeding before fragment isolation (72%) than after fragment isolation (11%). Unexpectedly, there was no significant decrease in the number of young birds after fragment isolation, although sample sizes for young were small (n = 43). This may have been due to sustained immigration of young birds to fragments after isolation. Together, our results provide some of the strongest evidence to date that avian breeding activity decreases in response to fragment isolation, which could be a fundamental mechanism contributing to ecosystem decay.
Dedifferentiated leiomyosarcoma of the uterus: a clinicopathologic and immunohistochemical analysis of 23 cases
Authors: Chapel David B , Maccio Livia , Bragantini Emma , Zannoni Gian F , Quade Bradley J , Parra‐Herran Carlos , Nucci Marisa R 
Published: January 2023

AimsTo morphologically and immunophenotypically characterize dedifferentiated uterine leiomyosarcoma (LMS).Methods and resultsWe identified 23 dedifferentiated uterine LMS, defined as a malignant uterine smooth muscle tumour containing discrete differentiated and dedifferentiated components (i.e. with and without morphologic and immunophenotypic evidence of smooth muscle differentiation, respectively). The differentiated component was leiomyosarcoma in most cases (17/23), though some arose from a leiomyoma (n = 4) or smooth muscle tumour of uncertain malignant potential (n = 2). The dedifferentiated tumour component showed noncohesive polygonal cells with moderate to abundant cytoplasm, pleomorphic nuclei with coarse vesicular to smudged chromatin, one or more macronucleoli, frequent multinucleation, and atypical mitoses. Three cases showed heterologous osteosarcomatous or chondrosarcomatous differentiation. Immunohistochemistry revealed alterations characteristic of uterine LMS, including Rb loss (18/19); strong diffuse p16 (17/19); strong diffuse (9/19) or complete absence of (5/19) p53; and ATRX loss (6/16). Compared to a control cohort of uterine LMS without dedifferentiation, dedifferentiated uterine LMS showed significantly shorter disease‐specific (median, 54 versus 20 months; 5‐year DSS, 46% versus 36%; P = 0.04) and disease‐free (median, 31 versus 8 months; 5‐year DFS, 42% versus 8%; P = 0.002) survival. Of 19 dedifferentiated uterine LMS with follow‐up, 12 had died of disease at median 14 (range, 2–73) months; four were alive with disease at 4, 12, 44, and 50 months; and three were alive with no evidence of disease at 56, 109, and 114 months.ConclusionRoutine prospective recognition of dedifferentiated uterine LMS and distinction from mimics is advocated for accurate prognostication and for further characterisation of these tumours.
Recent patent applications for coffee and coffee by‐products as active ingredients in cosmetics
Authors: de Mello Valéria , de Mesquita Júnior Gilmar Alves , Alvim Julia Gabriela Eurico , Costa Juliana de Carvalho da , Vilela Fernanda Maria Pinto 
Published: January 2023

AbstractObjectiveCoffee is one of the most consumed beverages worldwide, and its production and consumption generate large amounts of by‐products annually. Coffee by‐products and coffee beans are rich in bioactive compounds of great commercial value, including potential applications as active ingredients in skin care products and cosmetic formulations. In addition, there has been growing interest in the use of natural ingredients for cosmetic purposes. Considering the importance of coffee in the world economy, its chemical constituents with potential for cosmetic and dermatological application, and the importance of patents for innovation and technological development, the present study aimed to review recent patents involving coffee and coffee by‐product use in cosmetics.MethodsThis review was carried out using Espacenet. The following inclusion criteria were established: patents that included the terms “coffee” and “skin” in the title, abstract and claims and belonged to the classification A61Q, which is related to the “specific use of cosmetics or similar toilet preparations” considering the International Patent Classification (IPC) or Cooperative Patent Classification (CPC).ResultsConsidering the 52 patents analysed, the bean was the main way to obtain extracts (39), followed by green beans (7), silverskin (3), peel and pulp (1), pulp (1) and beans and leaves (1). The formulations are mainly intended for use in nonspecific areas of skin (29), eye areas (12), scalp hair (9) and lip skin (2) with claims of anti‐ageing, moisturizers, sun protection, hair growth, anti‐dandruff, etc.ConclusionCoffee and its residues have high amounts of phenolic compounds, caffeine, fatty acids and other substances known to have important biological properties for the skin. Coffee and its by‐products are promising ingredients to be incorporated into topical formulations, ensuring skin health benefits and reducing the environmental impact.
Dysphoric milk ejection reflex (D‐MER) and its implications for mental health nursing
Authors: Frawley Timothy , McGuinness Denise 
Published: January 2023

AbstractDysphoric milk ejection reflect (D‐MER) is a dysphoria which women may experience within seconds of commencing breastfeeding. It is only recently gaining recognition in the academic literature and may have important implications for breastfeeding continuation, differential diagnosis and perinatal mental health. This perspective piece introduces the topic, sets out the physiological processes underpinning the experience and outlines why increased awareness of D‐MER is important for the profession of mental health nursing.