bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of Extension and Advisory Services Provided by Input Dealers during COVID-19 Pandemic
Authors: Mathuabirami V. , Karthikeyan C. , Devi M. Nirmala , Ganapati Patil Santosh , J. Paul Mansingh 
Published: July 2023

Aim: The main aim of this research is to study the input dealers’ perception towards the extension ad advisory services provided during COVID-19 pandemic.
Study Design: Cross section research design.
Place and Duration of Study: Coimbatore and Tiruvallur districts of Tamil Nadu, India. Two years (2021-2022).
Methodology: From the selected two districts 70 extension officials were randomly selected for conducting the study. Data were collected from the input dealers who delivered extension and advisory services to farmers during COVID-19 pandemic. Paper-Pencil survey method was used to collect data from the farmers. The survey approach is useful for evaluating opinions and trends by gathering quantitative data. A separate scale was developed for assessing the input dealer’s perception towards the effectiveness of extension and advisory services. CIPP model of evaluation was used for developing scale to measure the input dealer’s perception towards the EAS. The developed scale were validated by the experts. A scale was measured using 5-point Likert scale with 1 denoting "strongly disagree and 5 denoting “strongly agree” were employed in this study.
Results: Majority of the input dealers (70.00%) had medium to very high level of perception towards the effectiveness of extension and advisory services. Input dealer’s perceived that they possessed necessary skills and experience for delivering extension and advisory services. They also perceived that services were made available to farmers on time, door delivery of services were provided, increased agricultural production and productivity of farmers which lead to increased income of the farmers.
Conclusion: Effectiveness of extension and advisory services provided by input dealers during COVID-19 pandemic was effective in input dealer’s perception. Therefore policy makers must propose a policy in a way that government service provider will work along with the input dealers for delivering extension and advisory services to the farming community. Pluralistic extension approach will be the best option for extension service provider.
Evaluation of Genetic Diversity in Sodic Soil-grown Barnyard Millet (Echinochloa frumentacea (Roxb.) Link) Germplasm
Authors: Sampath L. , Dhanalakshmi R. , Kalaimagal T. , Susmitha D. , Antony B. J. 
Published: July 2023

Aims: Barnyard millet (Echinochloa frumentacea) is a significant tiny millet crop characterized by because of its high nutritional value, exceptional ability to withstand extremes of climate, and short duration. Globally, the area under salt affected soil is expanding. Therefore, genetic diversity analysis was conducted to identify genetically diverse genotypes for yield improvement under sodic condition.
Study Design: Randomised block design.
Place and Duration of Study: An experiment was carried under sodic soil condition at Anbil Dharmalingam Agricultural College and Research Institute, Trichy, Tamil Nadu.
Methodology: Ninety two genotypes of barnyard millet, including two commercial check varieties, MDU1 and CO (KV) 2. Plant height (cm), inflorescence length (cm), inflorescence width (cm), lower raceme length (cm), flag leaf length (cm), flag leaf width (cm), number of leaves on main tiller, number of productive tillers per plant, and grain yield per plant (g) were the nine quantitative traits that were recorded. GENRES software version 3.11 was used for analysing the data.
Results: For all of the analysed attributes, the genotypes showed significant differences based on the analysis of variance. Ninety two genotypes were divided into nine groups according to nine quantitative features using Mahalanobis D2-Statistics. Cluster II was the one with the greatest number with 25 genotypes, followed by cluster V with 22 genotypes, Cluster VI with 17 genotypes, Cluster IV with 11 genotypes, Cluster I with 8 genotypes, Cluster VIII with 4 genotypes, Cluster III and VII with only 2 genotypes per cluster, and Cluster IX with just 1 genotype. Cluster IV was second in terms of intra-cluster distance, after Cluster VIII. The largest inter-cluster distance was observed between clusters I (TNAUF01000021 - EF 37, TNAUF01000022 - EF 38, TNAUF01000022 - EF 39, etc.) and IX (MDU1).
Conclusion: Choosing these genotypes as parents from genetically diverse clusters for breeding programmes might produce heterotic hybrids that produce enough genetic diversity in barnyard millet genotypes under sodic soil conditions.
Lived Experiences of Patients Hospitalized With Acute Decompensated Heart Failure and Kidney Dysfunction
Authors: Bernacki Gwen M. , O’Hare Ann M. , Assefa Mahlet , O’Brien Kevin D. , Prince David K. , Kirkpatrick James N. , Bansal Nisha , Butler Catherine R. 
Published: January 2025

ImportanceFor patients hospitalized with acute decompensated heart failure (ADHF), the presence of kidney dysfunction can substantially shape prognosis and treatment options. Yet little is known about the lived experiences of these medically vulnerable patients.ObjectiveTo elicit accounts of the illness and care experiences of patients currently or recently hospitalized with ADHF and kidney dysfunction in order to identify potential opportunities to improve care.Design, Setting, and ParticipantsThis qualitative study of patients with ADHF and kidney dysfunction was conducted at 3 hospitals within an academic health system serving the Greater Seattle area between February 2022 and February 2023. Patients were recruited from those enrolled in a parent study of patients hospitalized with ADHF. A sample of patients who had either acute kidney injury and/or chronic kidney disease were invited to participate in 1 or more qualitative interviews during hospitalization or approximately 3 months postdischarge.Main Outcomes and MeasuresTranscribed interviews were analyzed using inductive thematic analysis.ResultsA total of 40 interviews were conducted among 28 participants. The mean (SD) participant age was 67 (12) years, and 8 (28%) identified as female, 4 as Black [14%], and 20 as White [71.4%]. Qualitative analysis resulted in 4 dominant themes: (1) pervasive impact of functional limitation, (2) adapting goals and changing expectations, (3) struggling to interpret clinical information, and (4) decisional roles and relationships with clinicians. Participants described how their illness had shaped their day-to-day function, life space, and sense of self. They did not always speak in explicit terms about prognosis but most recognized limits on longevity and life options and had started to rethink their priorities. Most participants were engaged in a process of trying to understand the medical aspects of their illness and care, often drawing on their own embodied experience of illness. They had particular difficulty grasping the significance of kidney dysfunction and connecting this to the symptoms they were experiencing. Participants did not always feel equipped or empowered to engage in clinical decision-making, tending to trust the clinicians caring for them.Conclusions and RelevanceInterviews with adults currently or recently hospitalized with ADHF and kidney dysfunction highlight the importance of improving education, communication, and engagement in care among members of this medically vulnerable group.
Biomimetic Fibrous Bone Substitute Manufacture Through Non‐Solvent‐Assisted 3D Printing
Authors: Zhang Kunxi , Fang Haowei , Cheng Xiangyang , Li Jinyan , Zeng Jiujiang , Zhang Tao , Cui Haiyan , Gu Huijie , Yin Jingbo 
Published: January 2025

AbstractThe manufacturing of biomimetic bone characterized by an organic–inorganic combination and fibrous structure has garnered significant attention. Inspired by the formation of multi‐layered fibrous structures in bone tissue, this study is based on the fibril assembled from poly(γ‐benzyl‐L‐glutamate) (PBLG) in helicogenic solvent, proposing a non‐solvent‐assisted 3D printing method for realizing the PBLG 3D printing while generating biomimetic fiber structures in One‐Step to mimic the formation of collagen fiber bundles. The unprintable mixture of PBLG and hydroxyapatite nanoparticles (nHA) in 1,4‐dioxane exhibits extrudability, self‐supporting properties, and plasticity in ethanol. Meanwhile, ethanol‐assisted printing leads to the spontaneous growth of PBLG‐fibrils into submicron‐fibers. Moreover, the integration of nHA with PBLG‐fibers through hydrogen bonding contributes to the improvement of printability and mechanical properties. This method of ethanol‐assisted fiber generation is successful with concentrated PBLG solutions, overcoming the limitation of previous research that focused only on dilute solutions. To expand the printable window, an ethanol‐gel is developed as a support to achieve omnidirectional printing, resolving the issue of interlayer collapse caused by gravity and the conflict between printability and biomimetic fibers generation, optimizing the biomimetic bone manufacturing, leading to the precise biomimetic design of bone structures.
Gravity‐Switch‐Triggered Triboelectric Nanogenerator for Multi‐Directional Wave Energy Harvesting
Authors: Huo Da , Yang Xinglin , Pan Yijiang , Su Jianye 
Published: January 2025

AbstractAs global energy demands increase, reliance on fossil fuels intensifies environmental and resource pressures, driving the exploration of renewable sources. With its high energy density and potential to meet global energy needs, wave energy attracts significant attention. Despite the abundance of wave energy resources, efficiently harvesting multi‐directional wave energy remains challenging. This study introduces a gravity‐switch‐triggered triboelectric nanogenerator (GS‐TENG) designed to enhance the efficiency of wave energy collection. The GS‐TENG uses a gravity switch to control circuit connections and disconnections, optimizing the internal structure and experimentally adjusting the number of nylon balls to enhance output performance. In experiments, the GS‐TENG demonstrated adaptability to various wave directions and achieved a short‐circuit current of 22.2 µA, an open‐circuit voltage of 196 V, and a transferred charge of 75 nC under specific conditions. This showcases its superior performance in low‐frequency wave energy collection. Additionally, the device successfully lit 100 LEDs in a simulated wave environment, validating its potential for practical applications. The research on GS‐TENG provides an effective approach for harvesting wave energy and lays significant theoretical and experimental foundations for further development of wave energy collection technologies.
Covalent Affibody‐Molecular Glue Drug Conjugate Nanoagent for Proximity‐Enabled Reactive Therapeutics
Authors: Gao Wenhui , Yang Xiaoyuan , Li Qingrong , Liu Yingchun , Huang Wei , Xia Xuelin , Yan Deyue 
Published: January 2025

AbstractSulfur‐fluoride exchange (SuFEx) reaction is an emerging class of click chemistry reaction. Owing to its efficient reactivity under physiological conditions, SuFEx reaction is used to construct covalent protein drugs. Herein, a covalent affibody‐molecular glue drug conjugate nanoagent is reported, which can irreversibly bind with its target protein through proximity‐enabled SuFEx reaction. As a proof of concept, a latent bioreactive unnatural amino acid fluorosulfate‐L‐tyrosine (FSY) is first introduced at site 36 of the affibody with cysteine mutation (ZHER2:342‐Cys) to produce ZHER2:342‐36FSY‐Cys. Subsequently, ZHER2:342‐36FSY‐Cys is coupled with a molecular glue drug (CR8) to yield an amphiphilic conjugate of ZHER2:342‐36FSY‐CR8, which can self‐assemble into affibody–drug conjugate nanoagent (ZHER2:342‐36FSY‐CR8 ADCN) in PBS. When ZHER2:342‐36FSY‐CR8 ADCN specific binds to human epidermal growth factor receptor 2 (HER2) on cancer cells, the FSY36 of ZHER2:342 approaches to the His490 of HER2 and ultimately reacts with each other to form a covalent bond via SuFEx reaction. Such a covalent binding mode endows ZHER2:342‐36FSY‐CR8 ADCN with permanent binding ability to effectively increase the concentration of drugs in tumor. Eventually, the covalent ZHER2:342‐36FSY‐CR8 ADCN exhibits an outstanding tumor inhibition ratio of 90.03 ± 4.29% in HER2‐positive ovary tumor models, strikingly higher than that of the noncovalent one (64.25 ± 7.71%).
Machine Vision Applications for Welfare Monitoring in Aquaculture: Challenges and Opportunities
Authors: Fitzgerald Amy , Ioannou Christos C. , Consuegra Sofia , Dowsey Andrew , Garcia de Leaniz Carlos 
Published: January 2025

ABSTRACTIncreasing consideration of welfare in aquaculture has prompted interest in non‐invasive methods of monitoring that avoid unnecessary stress and handling. Machine vision (MV) provides a potential solution to these needs, as it can be used for non‐invasive monitoring of animal health and welfare in real‐time. We examined the practical applications of MV for welfare monitoring in aquaculture, the hardware and algorithms used for automated data collection, and the main challenges and solutions for data processing and analysis. The most common application of MV has been the estimation of size‐related metrics (growth, biomass) in fish, but key aspects of welfare, such as monitoring of parasites and disease or detection of stress‐related behaviours, are lagging behind. Numerous camera setups have been used, ranging from single to stereoscopic cameras and from emersed to submerged cameras, but these have often been used under optimal conditions that may not always reflect those prevalent in industry (high densities, low visibility), likely overestimating performance. Object detection algorithms, such as YOLO, have been the approach of choice for most MV applications in aquaculture, but our review has identified an increasing number of alternatives that can help circumvent some of the challenges posed by high densities and poor lighting typical of commercial farms. MV has the potential to transform welfare monitoring in aquaculture, but there are still important challenges that need to be overcome before it can become mainstream, namely the ability to detect ectoparasites and diseases, identify abnormal behaviours, and work across taxa, particularly in crustaceans.
3D Rotary Wet‐Spinning (RoWS) Biofabrication Directly Affects Proteomic Signature and Myogenic Maturation in Muscle Pericyte–Derived Human Myo‐Substitute
Authors: Reggio Alessio , Fuoco Claudia , De Paolis Francesca , Deodati Rebecca , Testa Stefano , Celikkin Nehar , Volpi Marina , Bernardini Sergio , Fornetti Ersilia , Baldi Jacopo , Biagini Roberto , Seliktar Dror , Cirillo Carmine , Swieszkowski Wojciech , Grumati Paolo , Cannata Stefano , Costantini Marco , Gargioli Cesare 
Published: January 2025

ABSTRACTSkeletal muscle tissue engineering (SMTE) has recently emerged to address major clinical challenges such as volumetric muscle loss (VML). Here, we report a rotary wet‐spinning (RoWS) biofabrication technique for producing human myo‐substitutes with biomimetic architectures and functions. Here, we demonstrate how the proposed technique may be used to establish a well‐tailored, anisotropic microenvironment that promotes myogenic differentiation of human skeletal muscle–derived pericytes (hPeri). Using high‐resolution mass spectrometry–based proteomics with the integration of literature‐derived signaling networks, we uncovered that (i) a 3D biomimetic matrix environment (PEG‐fibrinogen) confers a less mitogenic microenvironment compared to standard 2D cultures, favoring the formation of contractile‐competent bundles of pericyte‐derived myotubes in an anchoring‐independent 3D state and (ii) the RoWS method promotes an upregulation of muscle matrix structural protein besides increasing contractile machinery proteins with respect to 3D bulk cultures. Finally, in vivo investigations demonstrate that the 3D‐biofabricated myo‐substitute is fully compatible with the host ablated muscular tissue, exhibiting myo‐substitute engraftment and muscle regeneration in a mouse model of VML. Overall, the results show that RoWS offers a superior capability for controlling the myogenic differentiation process on a macroscale and, with future refining, may have the potential to be translated into clinical practice.
Sprayed Aqueous Microdroplets for Spontaneous Synthesis of Functional Microgels
Authors: Li Xin , Zhang Wenjing , Li Helin , Shuai Qi , Zhang Xingcai , Pich Andrij 
Published: January 2025

AbstractThe development of sustainable synthesis route to produce functional and bioactive polymer colloids has attracted much attention. Most strategies are based on the polymerization of monomers or crosslinking of prepolymers by enzyme‐ or cell‐mediated reactions or specific catalysts in confined emulsions. Herein, a facile solution spray method was developed for spontaneous synthesis of microgels without use of confined emulsion, additional initiators/catalysts and deoxygenation, which addresses the challenges in traditional microgel synthesis. The polarization of air‐water interface of the microdroplets can spontaneously split hydroxide ions in water to produce hydroxyl radicals, thereby initiating polymerization and crosslinking in air environment. This synthesis strategy is applicable to a variety of monomers and enables the fabrication of microgels with tunable chemical structures and variable sizes. Importantly, the synthesis route also allows for the preparation of enzyme‐ or drug‐loaded microgels via the in situ encapsulation, which also display high enzymatic activity and stimuli‐triggered drug release. Therefore, this work not only is of great significance to macromolecular science and microdroplet chemistry, but also may bring new insights into cellular biochemistry and even prebiotic chemistry due to the prevalence of microdroplets in the environment.
Ultrasonic‐Assisted Adsorptive Uptake of Malachite Green Dye Onto Zn/Cu‐Layered Double Oxide: Spectrophotometric and Smartphone‐Based Detection
Authors: Pandey Bhamini , Singh Poonam , Kumar Ravinder 
Published: January 2025

ABSTRACTIn this work, efforts have been made to synthesize novel Zn/Cu‐layered double oxide by performing calcination of Zn/Cu‐layered double hydroxide, which was later used as an adsorbent for the uptake of toxic azo dye. Malachite green (MG) was used as a model cationic dye for assessing the practicability of dye wastewater treatment. From batch experiments study, it was evident that ultrasonication‐assisted adsorption enables 90% of the MG to be removed from water within 30 min at neutral pH conditions. The ultrasound‐assisted sorption process was driven by the acoustic cavitation process, which facilitated a homogeneous distribution of active adsorption sites on surface of the adsorbent resulting efficient removal of dye molecules. As predicted by the Langmuir isotherm model, the sorbent exhibited maximum adsorption capacity of 128.20 mg/g, and based on kinetic measurements, the pseudo‐second‐order adsorption model was found to be most accurate. The kinetic studies also revealed that intraparticle diffusion is the rate‐limiting step of the adsorption process. The current study also demonstrates smartphone‐assisted estimation of adsorbed MG dye in order to provide a simple, convenient, cost‐effective, and instrument‐free method to quantify dye concentration. A smartphone‐based app called “ColorPicker” was used to calculate MG dye adsorption rate in real time based on RGB values and colorimetric responses. The percent removal obtained using smartphone app (87%) was in agreement with the data obtained using UV–vis spectroscopy (90%). As a result of its excellent properties, Zn/Cu‐LDO appears to be a potential candidate for wastewater remediation.
Highly Sensitive Spectrofluorimetric and Colorimetric Quantitation of Neomycin Using Nanosilver‐Fluorescein Hybrid: A Comprehensive Assessment of Greenness and Blueness
Authors: El‐Didamoony Mai A. , Elkady Ehab F. , Sayed Rawda M. , Batakoushy Hany A. , Mostafa Eman A. 
Published: January 2025

ABSTRACTTwo versatile yet simple methods, colorimetric and spectrofluorimetric, were utilized for the quantitation of nonchromophore neomycin using silver nanoparticles modified with fluorescein. Fluorescein was excited at 485 nm (emission at 515 nm); when it is deposited on the surface of silver nanoparticles, its fluorescence intensity at 515 nm is quenched. Neomycin restores the fluorescence level at 515 nm by displacing fluorescein from nanoparticle binding sites. When neomycin reacts with silver nanoparticles modified with fluorescein, it causes reduction of silver nanoparticles' plasmon resonance surface band at 395 nm and changes of the solution's color from yellow to pale pink. For the spectrofluorimetric and colorimetric techniques, the calibration graphs with a linearity range of 5–45 ng/mL and 0.5–10 μg/mL, respectively. The suggested techniques were used to determine neomycin in neomycin tablets, yielding mean recovery of 99.696 ± 0.764 and 99.925 ± 1.515 for the spectrofluorimetric and colorimetric methods, respectively. The established techniques were validated according to the ICH guidelines. Analytical greenness calculator (AGREE) along with greenness tool for sample preparation (AGREEprep) was used to evaluate how environmentally friendly the sample preparation and analytical process were. In addition, the suggested approaches' practicality is assessed using the Blue Applicability Grade Index (BAGI).
Drivers of Palliative Care and Hospice Use Among Patients With Advanced Lung Cancer
Authors: Edmonds Megan C. , Mazor Melissa , Jain Mayuri , Li Lihua , Augustin Marsha , Morillo José , Allen Olivia S. , Avril Amina , Wisnivesky Juan P. , Smith Cardinale B. 
Published: January 2025

ABSTRACTPurposeDespite rigorous evidence of improved quality of life and longer survival, disparities in the utilization of palliative and hospice care persist for racial and ethnic minority patients with cancer. This study evaluated the impact of psychosocial factors on utilization of these services.MethodsPatients with advanced lung cancer were recruited at a large academic urban hospital. Patients were surveyed about their knowledge of palliative care and hospice and their beliefs regarding medical mistrust, lung cancer care, palliative care and hospice. We used univariate and multivariable logistic regression analyses to examine the association between mistrust, knowledge and beliefs among the entire cohort and minority (Black and Hispanic) and non‐minority patients on utilization of palliative care consultation and hospice care use.ResultsNinety‐nine of the enrolled participants had a mean age of 64 years. Minority patients were more likely to receive a palliative care referral (p < 0.001) and attend a consult (p = 0.003). Similarly, they were more likely to receive a hospice referral (p = 0.04), however there was no difference in hospice care use based on minority status (p = 0.102). In our adjusted model, older patients and those reporting negative lung cancer beliefs were more likely to receive hospice care (OR: 1.06, 95% CI: 1.004–1.138; OR: 1.04, 95% CI: 1.002–1.093, respectively).ConclusionMinority patients with advanced lung cancer were more likely to receive a palliative care referral and specialty level consultation when compared to non‐minority patients. Our work highlights the importance of proactive referral processes in facilitating access to palliative and hospice services, particularly among younger patients.