bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polyoxidovanadates [MoVIVV9O28]5- and [H2PtIVVV9O28]5- interact with CHO cell plasma membrane lipids causing aggregation and activation of a G protein-coupled receptor
Authors: Kostenkova Kateryna , Althumairy Duaa , Rajan Ananthu , Kortz Ulrich , Barisas B. George , Roess Deborah A. , Crans Debbie C. 
Published: April 2023

Mono substituted heteropolyoxidovanadates, when compared to effects of a corresponding isopolyoxidovanadate (POV), were found to be more effective initiators of signal transduction by a G protein-coupled receptor (GPCR), specifically the luteinizing hormone receptor (LHR). Here we report that LHRs signal productively when CHO cells expressing the receptor are treated with two heteropolyoxidovanadates PtIV in monoplatino(IV)nonavanadate(V) ([H2PtVIVV9O28]5-, V9Pt), and MoIV in monomolybdo(VI)nonavanadate(V) (Mo[VIVV9O28]5-, V9Mo). Both substituted decavanadate derivatives were more effective than decavanadate which is more charged, has greater stability and forms the [V10O28]6- anion (V10) in cell culture medium at pH 7.4. For viable CHO cells expressing 10 k or 32 k LHR/cell and treated with 11 μM V9Pt and 13 μM V9Mo, mono substituted heteropolyoxidovanadates significantly decreased the packing of plasma membrane lipids for about 1 h. This brief change in membrane structure was accompanied by increased aggregation of LHR and cell signaling as indicated by increased intracellular levels of cAMP. More pronounced changes in lipid packing and LHR signaling were associated with short acting heteropolyoxidovanadates than with the more stable V10. When LHR was overexpressed, V9Pt and V9Mo had little or no effect on membrane lipid packing or receptor aggregation and the LHR was constitutively activated as indicated by elevated intracellular cAMP levels. Speciation of V9Pt and V9Mo in H2O and cell medium was monitored using 51V NMR spectroscopy and confirmed that V9Pt and V9Mo had greater effects on CHO cells despite decomposing more rapidly in the cell growth medium. Thus, under conditions that promote CHO cell growth, V9Pt and V9Mo, despite their smaller molecular charge and their reduced stability, favor LHR signaling over that induced by V10. Importantly, under the same experimental conditions, CHO cells treated with V9Pt and V9Mo do not exhibit as strong toxic effects observed for cells treated with the longer lived V10. In summary, unlike the longer lived V10 which is more growth inhibitory to cells, monosubstituted heteropolyoxidovanadates are more effective in transiently initiating signaling by a G protein-coupled receptor but, because of rapid hydrolysis, inhibit cell growth less.
The integrative analysis based on super-enhancer related genes for predicting different subtypes and prognosis of patient with lower-grade glioma
Authors: Hu Yungang , Yang Qingqing , Cai Shuzhou , Wang Wei , Fu Shiyin 
Published: April 2023

Objective: Emerging evidence revealed that super-enhancer plays a crucial role in the transcriptional reprogramming for many cancers. The purpose aimed to explored how the super-enhancer related genes affects the prognosis and tumor immune microenvironment (TIME) of patients with low-grade glioma (LGG).Methods: In this study, the differentially expressed genes (DEGs) between LGG cohorts and normal brain tissue cohort were identified by the comprehensive analysis of the super-enhancer (SE) related genes. Then non-negative matrix factorization was performed to seek the optimal classification based on the DEGs, while investigating prognostic and clinical differences between different subtypes. Subsequently, a prognostic related signature (SERS) was constructed for the comprehensive evaluation in term of individualized prognosis, clinical characteristics, cancer markers, genomic alterations, and immune microenvironment of patients with LGG.Results: Based on the expression profiles of 170 DEGs, we identified three SE subtypes, and the three subtypes showed significant differences in prognostic, clinicopathological features. Then, nine optimal SE-related genes were selected to construct the SERS through the least absolute shrinkage and selection operator Cox regression analysis. Survival analysis showed that SERS had strong and stable predictive ability for the prognosis of LGG patients in the The Cancer Genome Atlas, China Glioma Genome Atlas, and Remdrandt cohorts, respectively. We also found that SERS was highly correlated with clinicopathological features, tumor immune microenvironment, cancer hallmarks, and genomic alterations in LGG patients. In addition, the predictive power of SERS for immune checkpoint inhibitor treatment is also superior. The qRT-PCR results and immunohistochemical results also confirmed the difference in the expression of four key genes in normal cells and tumors, as well as in normal tissues and tumor tissues.Conclusion: The SERS could be suitable to utilize individualized prognosis prediction and immunotherapy options for LGG patients in clinical application.
Effect of inclined pile on seismic response of bridge abutments undergoing liquefaction—Induced lateral displacement: Case study of Nishikawa bridge in the 2011 Great East Japan earthquake
Authors: Xue Ying-Ying , Wang Xing-Gang , Cai Fei 
Published: April 2023

It is reported that the inclined pile could be either beneficial or detrimental for the abutment they support in the condition of liquefaction—induced lateral displacement. To clarify the effect of inclined pile on seismic response of bridge abutments undergoing liquefaction—induced lateral displacement, numerical analyses were carried out on the damaged abutment that was supported by inclined piles and that was displaced riverwards 100 mm–150 mm due to soil liquefaction during 2011 Great East Japan earthquake. Accordingly, a fully coupled dynamic effective stress finite element model was developed for the soil—pile—superstructure system. It was found that the shear failure of the bridge abutment was initialized from the inclined piles and then followed by the middle vertical pile. Moreover, the earthquake—induced liquefaction caused substantial lateral displacement of soils around the piles and thus dominated the backward rotation of the abutments supported by inclined piles. Additionally, the exclusion of the deck pinning effect may lead to a marked increase in the lateral displacement and rotation of abutments. If the abutment were supported by vertical piles, a much larger lateral displacement was expected and the promotion of the earth pressure behind the abutment was the main cause of the forward rotation of the piled abutment.
The efficacy and safety of Xueshuantong (lyophilized) for injection in the treatment of unstable angina pectoris: A systematic review and meta-analysis
Authors: Xi Junyu , Wei Ruili , Cui Xin , Liu Yi , Xie Yanming 
Published: April 2023

Objective: Xueshuantong (lyophilized) for injection (XST) is an effective botanical drug for treating unstable angina pectoris (UAP). However, a meta-analysis of XST combined with conventional treatment (CT) against UAP has not been conducted. Therefore, this study aimed to investigate the effectiveness and safety of XST combined with CT for UAP patients compared to CT alone.Methods: Randomized controlled trials (RCT) of XST in UAP patients were retrieved from the Cochrane Library, PubMed, Web of Science, EMBASE, CNKI, VIP, Wanfang, and Chinese Biological Medicine Database databases. A meta-analysis was performed using Revman 5.4 and Stata 16.0, and the quality of the included literature was evaluated based on the Cochrane risk-of-bias 2.0 (RoB2.0) tool. The aggregate 95% confidence intervals (CIs), mean difference (MD), and relative risk (RR) estimates were calculated. A GRADE assessment was performed using GRADEprofiler 3.6, and trial sequent analysis was performed using TSA 0.9.Results: Thirty-four studies involving 3,518 patients were included in the analysis. The combination of CT with XST improved the comprehensive clinical efficacy (RR = 1.22, 95% CI: 1.18–1.26, p &lt; 0.00001) and ECG improvement (RR = 1.24, 95% CI: 1.18–1.31, p &lt; 0.00001). The frequency of angina attacks was lower (MD = −0.73, 95% CI: −0.92 to −0.55, p &lt; 0.00001), and the duration was shorter (MD = −1.08, 95% CI: −1.44 to −0.72, p &lt; 0.00001) in the group that received CT combined with XST compared to the one without XST. Total cholesterol levels (MD = −1.30, 95% CI: −1.83 to −0.78, p &lt; 0.00001) and triglyceride levels (MD = −0.76, 95% CI: −0.93 to −0.59, p &lt; 0.00001) were lower in patients who received CT in combination with XST than those who received CT alone. CT combined with XST reduced whole blood viscosity (MD = −0.72, 95% CI = −0.99 to −0.44, p &lt; 0.00001) and plasma viscosity (MD = −0.24, 95% CI: −0.46 to −0.03, p = 0.03). There was no statistically significant difference in the incidence of cardiovascular events or adverse events among patients treated with the combination of XST and CT compared to CT alone. The GRADE assessment indicated that the composite quality of the evidence was low. The trial sequent analysis showed an adequate sample size and stable findings for the clinical efficacy of CT combined with XST for unstable angina.Conclusion: The present systematic review and meta-analysis conditionally indicate that XST combined with CT improved the clinical outcomes of patients with unstable angina more than CT alone with a better safety profile. However, the results need further validation due to limitations in the quality of the included studies.Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022357395.
Podocytes are likely the therapeutic target of IgA nephropathy with isolated hematuria: Evidence from repeat renal biopsy
Authors: Luo Mian-Na , Yin Yanqing , Li Shangmei , Hao Junfeng , Yao Cuiwei , Xu Yong-Zhi , Liu Hua-feng , Yang Lawei 
Published: April 2023

Background: The present study aimed to prove the progression of immunoglobulin A nephropathy (IgAN) patients with isolated hematuria based on repeat renal biopsy data for the first time.Methods: 29 IgAN patients with isolated hematuria who received repeat renal biopsies were analyzed retrospectively, while 29 non-isolated hematuria IgAN patients with similar age and background were randomly selected as the control group. Clinical parameters were collected at the time of biopsy. The treatment strategies (conservative treatment with RASS blocker or immunosuppressive treatment) were choosen according to the pathological results at the first renal biopsy. The activity and chronicity indexes of renal lesions were evaluated. Markers of cell inflammation and proliferation were tseted by immunochemistry. The ultrastructure of podocytes was observed by transmission electron microscopy (TEM). Podocyte and oxidative stress marker (NPHS2 and 4-HNE) were detected by immunofluorescence.Results: The IgAN patients with isolated hematuria had better clinical indicators than those with no-isolated hematuria, such as better renal function, higher albumin and lower uric acid. The interval between two biopsies in IgAN patients with isolated hematuria was 630 (interquartile range, 409.5–1,171) days. The hematuria of the patients decreased significantly from 30 (IQR, 4.00–35.00) RBC/ul in the first biopsy to 11 (IQR, 2.50–30.00) RBC/ul in the repeated biopsy (p &lt; 0.05). The level of triglyceride decreased significantly (p &lt; 0.05). The other clinical indicators were not statistically significant (p &gt; 0.05). Deposits of IgA and C3 in the glomerulus were persistent. The activity index decreased, especially cellular crescent formation, while the chronicity index increased. The ultrastructure of podocytes was improved after treatment. The oxidative stress products of podocytes reduced after treatment.Conclusion: Although the clinical indicators of the IgAN patients with isolated hematuria were in the normal range, various acute and chronic pathological changes have occurred, and irreversible chronic changes have been progressing. Cell inflammation and proliferation persisted. Oxidative stress of podocytes was likely to be the therapeutic target. This study provided a strong basis for the progress of IgAN with isolated hematuria through pathological changes before and after treatment. This study will help clinicians recognize the harm of hematuria, change the traditional treatment concept, and help such patients get early treatment.
Nurses in China lack knowledge of inhaler devices: A cross-sectional study
Authors: Xie Ning , Zheng Zheng , Yang Qilian , Li Man , Ye Xiaofen 
Published: April 2023

Objective: To understand the level of knowledge about inhaler devices among medical staff.Methods: This study evaluated the knowledge of inhalation therapy and the use of inhaler devices among nurses in China. We administered a new self-designed online questionnaire to 1,831 nurses. The questionnaire comprised 11 questions, including the storage location of inhaler devices, steps involved in using inhaler devices, and common errors when using various devices.Results: Among the 1,831 participants, 816(44.57%), 122(6.66%), and 893(48.77%) nurses worked in community, secondary, and tertiary hospitals, respectively. Adequate knowledge of inhaler devices was demonstrated by 20.10%, 8.20%, and 13.10% of nurses working in community, secondary, and tertiary hospitals, respectively. Of the nurses working in community hospitals, 27.70% knew the key points for using inhalers compared to 15.57% in secondary hospitals and 23.18% in tertiary hospitals (p &lt; 0.01). Only 9.50%–26.00% of participants chose correct answers to the 9 questions about the use of inhalers. The accuracy rate of the responses was generally low, and the highest accuracy rate was 26.00%.Conclusion: Knowledge of inhalation therapy was better among nurses working in community hospitals than among those working in high-level hospitals. This is because of the clearer division of work and higher workload in high-level hospitals. Overall, nurses’ knowledge of inhalation therapy is low. Furthermore, knowledge about inhaler devices should be strengthened among nurses in Chinese hospitals. It is necessary to create training opportunities for nurses in China to increase their awareness and knowledge regarding the management of chronic respiratory diseases.
Evaluation of clinical trials of ethnomedicine used for the treatment of diabetes: A systematic review
Authors: Elmi Gul Rehman , Anum Kamil , Saleem Kalsoom , Fareed Rameesha , Noreen Sobia , Wei Haiyan , Chen Yongxing , Chakraborty Avirup , Rehman Masood Ur , Liyuan Shi , Abbas Muhammad , Duan Yongtao 
Published: April 2023

Diabetes mellitus (DM) is a widespread metabolic disorder with a yearly 6.7 million deaths worldwide. Several treatment options are available but with common side effects like weight gain, cardiovascular diseases, neurotoxicity, hepatotoxicity, and nephrotoxicity. Therefore, ethnomedicine is gaining the interest of researchers in the treatment of DM. Ethnomedicine works by preventing intestinal absorption and hepatic production of glucose as well as enhancing glucose uptake in muscles and fatty tissues and increasing insulin secretion. A variety of plants have entered clinical trials but very few have gained approval for use. This current study provides an evaluation of such clinical trials. For this purpose, an extensive literature review was performed from a database using keywords like “ethnomedicine diabetes clinical trial”, “clinical trials”, “clinical trial in diabetes”, “diabetes”, “natural products in diabetes”, “ethno-pharmacological relevance of natural products in diabetes”, etc. Clinical trials of 20 plants and natural products were evaluated based on eligibility criteria. Major limitations associated with these clinical trials were a lack of patient compliance, dose-response relationship, and an evaluation of biomarkers with a small sample size and treatment duration. Measures in terms of strict regulations can be considered to achieve quality clinical trials. A specific goal of this systematic review is to discuss DM treatment through ethnomedicine based on recent clinical trials of the past 7 years.
FEATURES OF FORMATION OF FUTURE MUSIC TEACHER’S COMPETENCE IN WORKING WITH CHILDREN'S CHOIR
Authors: Yunda V. 
Published: April 2023

The article reveals the peculiarities of the formation of future music teachers' competence in working with children's choirs. It is emphasized that the formation of future professionals' competence is an essential condition for the development of the musical culture of the younger generation, children's interest in the traditions of the musical culture of the Ukrainian people's creative music, and vocal and choral work in children's choir promotes musical abilities and emotional sphere. It is proven that the professional training of future music teachers to work with children's school choirs should be creative, taking into account individual capabilities, music theory, and performance training of students. It is revealed that the future music teacher must be prepared for various types and forms of creative vocal and choral work with children of different school ages, in which the future music teacher will perform as an educational institution, accompanist, accompanist, and illustrator.
It is emphasized that the modern school needs new forms and methods in the organization of musical activity for schoolchildren, which will promote the development of musical culture and interest of pupils in musical creativity.
METHODICAL PREREQUISITES FOR TRAINING MUSIC TEACHERS TO TEACH ART TO PUPILS
Authors: Ying Yue 
Published: April 2023

The purpose of the article is to analyze the requirements of general school education regarding the education of art students as determinants of the professional future music teacher methodical training. Scientific sources of Ukrainian and Chinese art pedagogy were involved in the theoretical analysis. The analysis was carried out in the context of identifying the potential of the achievements of Ukrainian artistic pedagogy and science for their possible introduction into the Chinese music and music-pedagogical education system, which is currently being integrated into the world's cultural process. The methods of theoretical analysis are used to determine the essence of the fundamental definition of the music teacher's readiness to teach art students, comparing the positions of scientists to reveal the essence of the requirements for the complex of methodical training of music teachers in Ukraine and China. The analysis of music teacher training requirements in the Ukrainian education system is based on the conceptual ideas of the New Ukrainian School. The differences in the ways of musical and pedagogical education formation in China in the context of historical and cultural processes and national specificities are shown. The joint determinant of the successful teacher-musician's professional activity in the direction of integrative art education of pupils is formulated. It is multi-artistic readiness centered on musical art and systematically encompasses other types of art in practical creativity. The qualities of a music teacher, which are aimed at successful professional activity, are described: as universal, musical and professional (professional musicianship and music-pedagogical), and personal. The content structure of teacher-musician training in teaching art to pupils has been specified, which includes artistic-reflective, artistic-empathic, motivational-value, artistic-active, and artistic-communicative components. Emphasis is placed on increasing the requirements for the spiritual and personal development of the music teacher in the regulatory documents and practice of Chinese music-pedagogical education.
CONTEMPORARY BLENDED LEARNING BACKGROUNDS OF TEACHING ENGLISH FOR LAW ENFORCEMENT
Authors: Loputko O. , Bohutskyi V. , Romanov I. 
Published: April 2023

The article is devoted to the implementation of contemporary blended learning backgrounds into teaching English for specific purposes in the higher educational establishments of the Ministry of Internal Affairs of Ukraine. The analysis of the method’s usage in the special conditions of the war state is introduced. It is underlined that the implementation of such kind of technology will benefit not only the possibility to hold an educational process in the distant form in stressful conditions but also give the opportunity to the formation of a specific discipline’s knowledge using inter­disciplinary ways, to support the skills and abilities of the active self and group work.
The contemporary background of using blended learning technology is worked out in the paper. Special attention is paid to the respectful points of the international language learning centers that work in the sphere of new technologies implemented into teaching English in higher educational establishments. The peculiarities of the special cadets and officers’ regimes of study and daily routine are analyzed, and their advantages and disadvantages for the organization of the educational process are implemented in the process of the model’s building. Many possibilities to make the model flexible with the help of supporting the educational process through the different structural elements and units of the educational establishment are introduced. Active participation and correlation of all the members of the educational process on each level of the model’s organization are considered to be the central idea of the study. The organization of interdisciplinary events and the realization of creative and scientific directions of the educative work are also crucial for the successful realization of the model.