bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revisiting Photocatalytic CO2 Reduction to Methanol: A Perspective Focusing on Metal‐Organic Frameworks
Authors: Fernandes de Almeida Vitor , Navalón Sergio , Dhakshinamoorthy Amarajothi , Garcia Hermenegildo 
Published: February 2025

AbstractPhotocatalytic CO2 reduction to CH3OH, particularly with metal‐organic frameworks (MOFs) as photocatalysts, has garnered significant attention due to its long‐term potential to harness sunlight for converting CO2 into a valuable fuel and chemical feedstock. Numerous studies in the literature report the successful formation of CH3OH from photocatalytic CO2 reduction, sometimes supplemented with sacrificial agents, with claims substantiated by isotopic labelling measurements. However, in this Scientific Perspective, we note that much of the existing evidence has not been obtained under sufficiently rigorous experimental conditions to conclusively confirm the formation of a highly reactive product like CH3OH from the chemically stable CO2 molecule. This Scientific Perspective outlines best practices designed to provide robust evidence for CH3OH formation in photocatalytic processes, which could be instrumental in clarifying the state‐of‐the‐art and accelerating the development of this technology toward practical applications.
Aging Behavior of PEEK, PTFE, and PI Insulation Materials Under Thermal Oxidative and Humid Conditions for Aerospace Applications
Authors: Ahmad Jawad , Niasar Mohamad Ghaffarian 
Published: February 2025

ABSTRACTPolyether ether ketone (PEEK), polytetrafluoroethylene (PTFE), and polyimide (PI) are widely used in aerospace due to their excellent properties. Understanding their aging behavior is essential for long‐term performance in extreme environments. This study examines the effects of thermal oxidative aging (at 250°C over varying periods) under humid conditions on their chemical, structural, thermal, mechanical, and dielectric properties. In PEEK, aging led to solidification and crosslinking phenomenon which resulted in increased tensile strength and storage modulus, while elongation at break and tan δ decreased. Dielectric permittivity, polarization charge density, and leakage current also declined with aging, while AC breakdown strength increased by 1.6% in PEEK. PTFE exhibited surface oxidation, thermal degradation, and a decrease in storage modulus, with an increase in loss tangent. Breakdown strength slightly decreased, while dielectric loss and leakage current increased over aging time. PI underwent severe mechanical degradation, with tensile strength reduced by 54% and elongation by 16%, along with oxidation‐induced discoloration. Low‐mass polar molecules generated in PI during thermal degradation which contributed to the deterioration of its dielectric properties, lead to increased permittivity, polarization, leakage current, and a lower breakdown strength observed after aging. These findings provide insights into degradation mechanisms, aiding aerospace material selection for extreme environments.
Robotic reconstruction of complex bladder neck stenosis: Single‐centre experience with three techniques
Authors: Rinderknecht Emily , Engelmann Simon Udo , Saberi Veronika , Haas Maximilian , Kälble Sebastian , Eckl Christoph , Hartmann Valerie , Goßler Christopher , Pickl Christoph , Denzinger Stefan , Burger Maximilian , Bründl Johannes , Mayr Roman 
Published: February 2025

AbstractObjectivesTo evaluate and compare surgical techniques for robot‐assisted reconstruction of recurrent bladder neck stenosis (BNS). BNS following a simple prostatectomy represents a rare but challenging condition in operative urology. Various robotic reconstructive techniques have been described, showing differing success rates. This monocentric case series reports on three distinct robotic surgical approaches for managing recurrent BNS.Patients and methodsA retrospective analysis was conducted on patients undergoing robot‐assisted surgical repair for recurrent BNS at our institution. Clinical data, including patient history, comorbidities (Charlson Comorbidity Index), surgical treatment, complications (Clavien‐Dindo classification) and follow‐up outcomes, were analysed.ResultsA total of 27 patients underwent robotic bladder neck reconstruction for recurrent BNS. Twelve patients were treated with YV plasty, 12 with stricture resection and end‐to‐end anastomosis and 3 with reconstruction using a buccal mucosa graft (BMG). At a median follow‐up of 18 months, therapy failure occurred in 9 patients (33.3%), with failure rates of 25.0% for YV plasty, 33.3% for stricture resection and 66.7% for BMG plasty. Nine patients (33.3%) experienced surgery‐related complications, including 7 minor complications (5 in the stricture resection group, 1 in the YV plasty group and 1 in the BMG group) and 2 major complications (1 in the stricture resection group and 1 in the YV plasty group). De novo incontinence occurred in five patients (19.2%), all of whom had undergone stricture resection with end‐to‐end anastomosis.ConclusionsRecurrent BNS poses a significant surgical challenge. Based on our experience, BMG reconstruction demonstrated suboptimal outcomes, while stricture resection was associated with the highest complication rate and the most frequent occurrence of de novo incontinence. YV plasty, with its relatively low morbidity and minimally invasive nature, has become the preferred technique in our institution for managing this condition. Prospective studies with larger cohorts are warranted to confirm these findings and further refine surgical approaches.
Enhancing the Learning Process in Structural Dynamics Through Jupyter Notebooks: A Project‐Based Learning Approach
Authors: Ledezma‐Ramírez Diego Francisco 
Published: February 2025

ABSTRACTNumerical simulation is nowadays an essential tool for different engineering disciplines, and in the case of mechanical vibrations and structural dynamics, it finds several important applications not only in theoretical approaches but also in virtual experiments and data analysis. However, most of the commercial applications are expensive or limited for student use, and as a result, open source and free alternatives have become popular in academia. Jupyter is an open‐source project that allows for the creation of interactive notebooks using Python, which is a powerful learning tool for engineering learning. In this paper, a new strategy for the course of structural dynamics is discussed, implementing the use of Jupyter notebooks, considering topics such as signal analysis, and free and forced vibration of systems with one and several degrees of freedom, among others. These notebooks combine background theory, text, images, and interactive code. Apart from being used as a learning tool with provided examples, the students are encouraged to use this tool to develop their reports in an integrative project. The description of the process and case study is presented, and the student learning process is assessed through anonymous surveys, demonstrating a great level of engagement and motivation resulting from the implementation of the strategy.
Improved Quantification of Circulating Tumor DNA in Translocation‐Associated Myxoid Liposarcoma by Simultaneous Detection of Breakpoints and Single Nucleotide Variants
Authors: Schmid A. , Lausch U. , Runkel A. , Kiefer J. , Pauli T. , Boerries M. , Bogner B. , Eisenhardt S. U. , Braig D. 
Published: February 2025

ABSTRACTBackgroundMyxoid liposarcomas (MLS) can exhibit a disseminated metastatic pattern, necessitating extensive diagnostics during follow‐up. With no tumor markers available, early diagnosis of recurrences and tumor monitoring is difficult. The detection of circulating tumor DNA (ctDNA; liquid biopsy) in MLS with the characteristic translocations t(12;16) and t(12;22) can provide an additional diagnostic. However, due to the often very low tumor fraction, distinguishing actual tumor variants from sequencing artifacts remains a key challenge.MethodsUsing MLS as a model for translocation‐driven tumors, this study evaluates a refined analytical approach for detecting both single nucleotide variants (SNVs) and structural variants (SVs) with the highest possible sensitivity and specificity. Different analysis pipelines using Unique Molecular Identifiers (UMIs) were compared in dilution series of tumor DNA from MLS patients (n = 11) and a cell line. The results were validated on plasma samples (n = 36) from two MLS patients and one patient with Synovial Sarcoma (SS).ResultsIn dilution series, the use of UMIs significantly reduced false positive events in SNV analysis while maintaining high sensitivity without significant differences. In SV analysis, the effect of UMIs was not consistently detectable, as some dilution series exhibited no false positive events even without UMI correction. Additional filter criteria further improved specificity without significantly compromising assay sensitivity. Validation on patient plasma samples confirmed these findings, demonstrating the advantages of the differentiated analytical approach.ConclusionBy integrating a refined analytical approach for SNVs and SVs, we achieved reliable ctDNA detection that corresponded with the clinical course of the patients’ disease. This method enables non‐invasive tumor detection in translocation‐driven tumors with low mutational burden and can easily be adapted into routine clinical diagnostics.
To Adopt or Adapt: A Scoping Review of Dialectical Behaviour Therapy With Patients With Minoritised Identity Constructs
Authors: Aunon Frances M. , Ingram Polly , Wolkowicz Noah , Martino Steve , Kacos Heather , Spana Ethan , Honsberger Mark , Rielage Jennifer Klosterman , Doran Neal , Carretta Rachel , Ridley Josephine , Giovannelli Thorayya Said , Bannister Jenny , Merians Addie , Howell Brittany , Demirelli Stacey , Smith Jason G. , Decker Suzanne E. 
Published: February 2025

ABSTRACTObjectiveDialectical behaviour therapy (DBT) is an evidence‐based psychological intervention (EBP) for reducing suicide behaviour. While DBT, like many EBPs, was developed and first evaluated in largely white samples in Western contexts, it is now broadly applied. This review assessed how DBT has been adopted or adapted when used with patient populations with minoritised identity constructs.MethodsWe conducted a PRISMA‐guided scoping review of articles using DBT with a minoritised patient population. We used an implementation science framework, Framework for Reporting Adaptations and Modifications‐Enhanced, to characterise adaptations to the content and context of DBT application. Through PubMed, Embase and PsycINFO, we identified 273 articles where the title or abstract mentioned DBT and described a minoritised adult patient population.Principle FindingsWe found 83.5% (228/273) articles reviewed reported adaptations to DBT content. Specifically, 66.3% (181/273), 44.6% (122/273) and 39.6% (108/273) of articles described reducing, expanding or changing DBT content, respectively. We found that 42.1% of articles (115/273) reported adaptations to the DBT context. Moreover, 57.5% of content adaptations and 65.3% of context adaptations did not specify a reason for adaptation.ConclusionsThis scoping review indicates that the majority of articles of studies using DBT with patients with minoritised identity constructs made adaptations to DBT, but few provided justification for these adaptations. We encourage clinicians and clinical administrators, researchers and policymakers to document adaptations and justifications thereof, and evaluate clinical and implementation outcomes.
Investigating T‐Cell Receptor Dynamics Under In Vitro Antibody‐Based Stimulation Using Imaging Flow Cytometry
Authors: Isozaki Akihiro , Kita Kazuma , Tiffany Ishii Natsumi , Oka Yuma , Herbig Maik , Yamagishi Mai , Wakamiya Tsubasa , Araki Taketo , Matsumura Hiroki , Harmon Jeffrey , Shirasaki Yoshitaka , Huang Kangrui , Zhao Yaqi , Yuan Dan , Hayashi Mika , Ding Tianben , Okamoto Yuji , Kishimoto Ayuko , Ishii Masaru , Yanagida Masatoshi , Goda Keisuke 
Published: February 2025

ABSTRACTT cells play a pivotal role in the immune system's response to various conditions. They are activated by antigen‐presenting cells (APCs) via T‐cell surface receptors, resulting in cytokine production and T‐cell proliferation. These interactions occur through the formation of immunological synapses. The advent of imaging flow cytometry has enabled detailed statistical analyses of these cellular interactions. However, the dynamics of T‐cell receptors in response to in vitro stimulation are yet to receive attention, despite it being a crucial aspect of understanding T‐cell behavior. In this article, we explore the responses of T cells to in vitro antibody‐based stimulation without APCs. Specifically, we established a Th1 cell clone, subjected it to a combination of centrifugation‐induced mechanical stress and anti–human CD3 and anti–human CD28 antibody stimulation as the in vitro antibody‐based stimulation, and captured and analyzed bright‐field and fluorescence images of single cells various hours after stimulation using an imaging flow cytometer. Our results indicate distinct temporal dynamics of CD3 and CD28. Notably, CD3 and CD28 relocated on the T‐cell surface immediately after stimulation, with CD3 receptors dispersing after 3.5 h, whereas CD28 remained clustered for 7.5 h. These receptor morphological changes precede cytokine production, suggesting their potential as early indicators of T‐cell activation.
Gender Disparities in Melanoma: Advances in Diagnosis, Treatment, and the Role of Artificial Intelligence
Authors: Kakish Diala Ra'Ed Kamal , Alsamhori Jehad Feras , Qaqish Lana N. , Aburumman Layan , Sarsur Razan , Salkhadi Asham Al , Nassif Zbeida Bassam , Akmal Mustafa Ahmed , Nashwan Abdulqadir J. 
Published: February 2025

ABSTRACTBackgroundMelanoma, a highly aggressive skin cancer, demonstrates significant gender disparities, with men facing later‐stage diagnoses, more aggressive tumor characteristics, and worse survival rates. This review examines the biological, behavioral, and environmental factors driving these disparities, alongside recent advancements in diagnosis and treatment. Additionally, it explores how artificial intelligence (AI) can address gender‐specific differences in melanoma incidence and outcomes.ResultsGender disparities in melanoma stem from biological factors, such as hormonal and genetic differences, and behavioral patterns like delayed health‐seeking among men. AI‐driven diagnostic tools, including convolutional neural networks (CNNs), show promise but often reflect biases in training data sets, underrepresenting darker skin tones and gender‐specific patterns. Ensuring diverse data sets, integrating “super‐prompts” or region‐specific demographic prompts, and utilizing bias‐aware algorithms can help mitigate these biases, thereby improving diagnostic accuracy and equity.ConclusionReducing gender disparities in melanoma requires integrating innovative technologies with equitable healthcare policies and education. Early detection using inclusive AI models tailored to diverse skin tones and genders, alongside targeted therapeutic strategies, is critical to improving outcomes for high‐risk groups, particularly men and underserved populations.
An analysis of contractile and protrusive cell behaviors at the superficial surface of the zebrafish neural plate
Authors: Araya Claudio , Boekemeyer Raegan , Farlie Francesca , Moon Lauren , Darwish Freshta , Rookyard Chris , Allison Leanne , Vizcay‐Barrena Gema , Fleck Roland , Aranda Millaray , Tada Masa , Clarke Jonathan D. W. 
Published: February 2025

AbstractBackgroundThe forces underlying convergence and internalization of the teleost neural plate remain unknown. To help understand this morphogenesis, we analyzed collective and individual cell behaviors at the superficial surface of the neural plate as internalization begins to form the neural keel in the hindbrain region of the zebrafish embryo.ResultsConvergence to the midline is not accompanied by anteroposterior elongation at this stage, and it is characterized by oscillatory contractile behaviors at the superficial surface of the neural plate, a punctate distribution of Cdh2 and medially polarized actin‐rich protrusions at the surface of the neural plate. We also characterize the intimate relationship and dynamic protrusive cell behaviors between the surfaces of the motile neural plate and the stationary overlying non‐neural enveloping layer.ConclusionsSuperficial neural plate cells are coupled by a punctate distribution of Cdh2‐rich adhesions. At this surface, cells tug on neighbors using oscillatory contractions. Oscillatory contractions accompany convergence and shrinkage of the cells' superficial surface for internalization during keeling. Some shrinkage for internalization occurs without oscillations. The deep surface of the overlying non‐neural enveloping layer is in contact with the superficial surface of the neural plate, suggesting that it may constrain the neural plate movements of convergence and internalization.
Controlling the Morphology of Carbon Xerogel by Diluents and Preparation Parameters for Supercapacitor Application
Authors: Medhavi Amit , Singh Vivek Kumar , Sharma Utkarsh Kumar , Singh Saras Prakash 
Published: February 2025

ABSTRACTThis work examined organic xerogels derived from resorcinol‐formaldehyde polycondensation and investigated bio‐alcohols as diluents. The concentrations of water, methanol, and ethanol in the precursor solution were shown to affect the porosity properties of the materials and their conductivity behavior. The xerogels synthesized with lesser catalyst and methanol as a diluent demonstrate enhanced properties. All samples exhibited an amorphous structure. A maximum double layer conductance of 50.28 F/g was observed for the sample synthesized at a formaldehyde to resorcinol ratio of 2.0 and a precursor to catalyst ratio of 200. The xerogel's pore volume and surface area grew significantly, reaching a BET surface area of 463.02 m2/g. XPS and TGA studies demonstrated a more pronounced graphitic nature in the xerogel. Electrochemical experiments showed relevant property for methanol‐mediated carbon xerogel. The results are noteworthy, as diluting agents decrease synthesis time, facilitating the fabrication of more competitive supercapacitor materials with better surface and porous properties.
Open Science in Impact Evaluation: What Impact Evaluators Can Learn From the Replication Crisis in Social Psychology
Authors: McNamee Ben , Forscher Patrick S. , Lenczner Michael 
Published: February 2025

ABSTRACTSince 2011, the academic field of social psychology has been undergoing a crisis of confidence in its results. This so‐called “replication crisis” has inspired a widespread examination of the practices that can lead to less‐than credible results, the incentives that inspire such practices, and the practices that could protect or enhance research credibility. We believe that the credibility of the field of impact evaluation may be similarly threatened by incentives to use poor research practices. We assess the history of both social psychology and impact evaluation and conclude that, although the source of the incentives for impact evaluators are different in kind than those for social psychologists, impact evaluators may be similarly incentivized to make their results look better than they really are. We review three parallels in the history of the two fields, including the use of research as sales, the pressurized, competitive environments in which the work takes place, and the use of research methods as rhetorical devices. Impact evaluators may be able to learn from the credibility‐enhancing solutions that worked for social psychology, though the field can consider new tools, such as standards, investment mechanisms, administrative data labs, and innovations in evidence‐sharing and synthesis. Rather than sleepwalking through a crisis we cannot see, impact evaluators should consider that their field may be in crisis, a crisis that may require widespread and collective efforts—a credibility renaissance—to discover and deploy methods to protect its legitimacy.
The Development and Application of a Method for the Detection of Viable but Nonculturable Staphylococcus aureus by Magnetic Bead Separation Combined With PMA‐qPCR
Authors: Wu Yong , He Jikun , Cai Zijian , Hsiao Hsin‐I. , Tang Junni , Chen Juan 
Published: February 2025

ABSTRACTStaphylococcus aureus is a major food‐borne pathogen and easy to be induced into a viable but nonculturable state under stress conditions from food processing, causing a significant and potential risk to public health. In this study, the primers for femB gene were examined to be specific to S. aureus. 10 μM of PMA was determined as the optimal treatment concentration to minimize the PCR amplification signal produced by dead bacterial DNA. When ∼107 CFU/mL of S. aureus was treated with sorbic acid, about 85% of S. aureus ATCC 6538 cells and 76% S. aureus‐1 cells were induced into VBNC state after 6 days, respectively. 1 mg/mL of Fe3O4 nanomagnetic beads, at 37°C for 10 min were determined as the optimal condition to separate VBNC S. aureus cells. The detection capability of Fe3O4‐PMA‐qPCR for VBNC S. aureus in rice, chicken, and milk was comparable with DNeasy PowerFood Microbial Kit‐PMA‐qPCR, and the detection efficiency of Fe3O4‐PMA‐qPCR was comparatively more excellent. Compared to commercial Kit‐PMA‐qPCR, the established Fe3O4‐PMA‐qPCR combined with plating assay was applied to quantify VBNC S. aureus inoculated into food samples, with advantages of specificity to S. aureus, short time, low cost, and sensitive results.
A MAZ::NCOA2 Subcutaneous Small Round Cell Sarcoma of Infancy With Diffuse S100/SOX10 Positivity: A Novel Entity
Authors: Chen Huiyao , Zhang Pu , Zhou Lingli 
Published: February 2025

ABSTRACTSmall round cell sarcomas (SRCSs) constitute a heterogeneous group of high‐grade tumors with a poor prognosis, predominantly affecting children and young adults. According to the 2020 WHO Soft Tissue Tumor classification, SRCSs are categorized into Ewing sarcoma, round cell sarcoma with EWSR1‐non‐ETS fusions, CIC‐rearranged sarcoma, and sarcoma with BCOR genetic alterations. Herein, we report a case of a 10‐month‐old boy presenting with a progressively enlarging left lumbar mass. Histopathological examination revealed a well‐demarcated lesion composed of small, round to oval cells with scant cytoplasm and mildly irregular nuclei. Immunohistochemical staining demonstrated strong, diffuse positivity for S100 and SOX10, indicating neurocristic differentiation. Next‐generation sequencing identified an in‐frame fusion between MAZ exon 3 on chromosome 16 and NCOA2 exon 12 on chromosome 8. Fluorescence in situ hybridization (FISH) confirmed a break‐apart signal at the NCOA2 locus. To the best of our knowledge, this represents the first documented instance of an NCOA2 rearrangement involving MAZ in SRCSs. This case broadens the molecular spectrum of SRCSs, highlights the importance of incorporating molecular techniques into diagnostic workflows, and may have implications for future diagnostic and therapeutic strategies.