bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endocytosis in Dictyostelium discoideum amoebae: Inhibition by cyclohexlmide and other inhibitors of protein synthesis
Authors: Gonzalez Carlos , Satre Michel 
Published: June 2023

ABSTRACT
Cycloheximide, an inhibitor of protein synthesis, inhibited endocytosis pathways in the vegetative amoebae of the cellular slime mold Dictyostelium discoideum. Half-maximal inhibition of fluid-phase pinocytosis in nutrient medium or in buffer occurred at 0.08 mM or 0.35 mM cycloheximide, respectively. Other inhibitors of eucaryotic protein synthesis inhibited fluid-phase pinocytosis, with the following IC50 in nutrient medium: 0.15 mM anisomycin, ImM emetine, 0.25 mM puromycin. Kinetic studies showed that the inhibitory effects of cycloheximide occurred rapidly, with a half-time of less than 10 min, and were fully reversible upon removal of the drug. Both fluid-phase pinocytic influx and efflux were inhibited by cycloheximide, which furthermore prevented the vanadate-induced efflux of fluorescein-labeled dextran, the fluid-phase marker used in these experiments. The massive secretion of lysosomal enzymes: acid phosphatase, N-acetylhexosaminidase or α-mannosidase, which follows overloading of the endosomal compartment by sucrose, was also inhibited by cycloheximide. All these phenomena were unimpaired by cycloheximide in the cycloheximide-resistant (cycAl) mutant strain X6.
Bidirectional Approach with PIPAC and Systemic Chemotherapy for Patients with Synchronous Gastric Cancer Peritoneal Metastases (GCPM)
Authors: Casella Francesco , Bencivenga Maria , Brancato Giorgio , Torroni Lorena , Ridolfi Cecilia , Puccio Carmelo , Alloggio Mariella , Meloni Francesca , Fusario Daniele , Marrelli Daniele , Giacopuzzi Simone , Roviello Franco , de Manzoni Giovanni 
Published: June 2023

Abstract
Background
This study evaluated the efficacy of pressurized intraperitoneal aerosol chemotherapy (PIPAC) with systemic chemotherapy as a bidirectional approach for gastric cancer (GC) patients with synchronous peritoneal metastases (SPM).

Methods
A retrospective analysis of a prospective PIPAC database was queried for patients who underwent a bidirectional approach between October 2019 and April 2022 at two high-volume GC surgery units in Italy (Verona and Siena). Surgical and oncological outcomes were analyzed.

Results
Between October 2019 and April 2022, 74 PIPAC procedures in 42 consecutive patients with Eastern Cooperative Oncology Group performance status ≤2 were performed—32 patients treated in Verona and 10 in Siena. Twenty-seven patients (64%) were female and median age at first PIPAC was 60.5 years (I–III quartiles: 49–68 years). Median Peritoneal Cancer Index (PCI) was 16 (I–III quartiles: 8–26) and 25 patients (59%) had at least two PIPAC procedures. Major complications according to the Common Terminology Criteria for Adverse Events (CTCAE; 3 and 4) occurred in three (4%) procedures, and, according to the Clavien–Dindo classification (>3a), one (1%) severe complication occurred. There were no reoperations or deaths within 30 days. Median overall survival (mOS) from diagnosis was 19.6 months (range 14–24), and mOS from first PIPAC was 10.5 months (range 7–13). Excluding cases with very heavy metastatic peritoneal burden, with PCI from 2 to 26, treated with more than one PIPAC, mOS from diagnosis was 22 months (range 14–39). Eleven patients (26%) underwent curative-intent surgery after a bidirectional approach. R0 was achieved in nine (82%) patients and complete pathological response was obtained in three (27%) cases.

Conclusions
Patient selection is associated with bidirectional approach efficacy and feasibility for SPM GC treatment, which may allow potentially curative surgical radicalization in highly selected cases.
Regulating Gender Diversity: Evidence from California Senate Bill 826
Authors: Allen Abigail , Wahid Aida Sijamic 
Published: June 2023

Do gender diversity quotas enhance firm value? This broad question has sparked significant debate because, whereas diversity is generally thought to improve board functioning, prior research in international settings suggests mandating diversity may have adverse consequences if it moves firms away from market equilibrium. Exploiting the political events leading up to the passage of California Senate Bill (SB) 826 as an exogenous shock to assess the market implications of board gender composition, we document either significantly positive or insignificant two-day abnormal returns for California firms across a variety of model specifications. Importantly, this finding contradicts the strong claims of contemporary studies of SB 826 that the California mandate was value-destructive. We reconcile these findings by providing critical evidence that contemporaneous results lack robustness to alternative model specifications and provide an incomplete picture by failing to comprehensively examine a broader set of event dates or account for mixed messaging on the final event date. We also provide some evidence of significant negative abnormal returns to litigation milestones that threaten to overturn SB 826 with the magnitude increasing in the size of board gender deficits. Finally, we provide evidence that there is no observable decline in newly appointed female director quality following the implementation of SB 826 relative to either preregulation or male director appointments. Collectively, our results suggest nonnegative (if not positive) consequences to California firms arising from SB 826 and directly counter assertions that mandated gender diversity is detrimental to firm value. This paper was accepted by Brian Bushee, accounting. Funding: The authors gratefully acknowledge research funding from Brigham Young University and the University of Toronto. Supplemental Material: The online appendix and data are available at https://doi.org/10.1287/mnsc.2023.4825 .
TÜRK HALK MÜZİĞİNDE VERSİYON (BENZER METİN) TERİMİNİN NEŞET ERTAŞ’A AİT İKİ ESER ÜZERİNDEN İNCELENMESİ
Authors: CAMGÖZ Nafiz 
Published: June 2023

Sözlü kültür ortamında aktarılan anonim ve ferdi yaratmalar folklorik oluşum süreci içinde çeşitlenebilmektedir. Bu çeşitlenmeler halkbilimi ürününün az ya da çok değişmesi anlamına gelmektedir. İleri düzeyde görülen bu farklılıklar için varyant (eş metin); aynı halkbilimi ürününün tekrarı sırasında oluşan küçük farklılıklar için ise versiyon (benzer metin) terimleri kullanılmaktadır. Neşet Ertaş’ın eserlerine versiyon terimi çerçevesinden bakıldığında, Ertaş’ın, aynı eseri farklı zamanlarda, farklı biçimlerde icra ettiğine rastlamaktayız. Bu farklılıklar eserin tümüne hâkim olan farklılıklar olmayıp, bazı müzik cümlelerinde ve eserin ara nağme (saz bölümü) kısımlarında görülmektedir. Bu küçük farklılıklar eserin kimliğini değiştirmediği gibi dinleyicide de farklı bir eser dinliyormuş hissiyatı uyandırmaz. Bu çalışmada, aynı kaynak kişinin, aynı eserinin farklı zamanlardaki ve farklı biçimlerdeki icrasını tanımlamak için versiyon (benzer metin) terimi kullanılmış olup Neşet Ertaş’a ait iki örnek eserin söz bölümlerinde yaptığımız müzikal karşılaştırmalar neticesinde versiyon teriminin somutlaştırılması amaçlanmıştır. Bu doğrultuda Neşet Ertaş’a ait “Zülüf Dökülmüş Yüze” adlı eserin iki versiyonu; “Neredesin Sen” adlı eserin ise dört versiyonu olduğu tespit edilmiştir. İncelenen örnek eserlerin sözlerinde bir değişikliğin olmadığı; sadece melodik ve ritmik yapılarında küçük farklılıkların olduğu görülmüştür ancak bu değişiklikler eserin bütününe hâkim olmadığı bir diğer ifadeyle eserin başkalaşmasına sebep olmadığı için versiyon terimi ile ifade edilmiştir.
Çalışmada, araştırma konusuyla ilgili önceden yapılmış çalışmalar incelenerek literatür taraması yapılmış ve dijital ortamdaki ses-görüntü kayıtları karşılaştırma yapılarak incelenmiştir.
Work-Based Learning Experiences and Students With Intellectual Disability
Authors: Rooney-Kron Magen , Dymond Stacy K. 
Published: June 2023

Abstract
The purpose of this study was to describe the extent to which students with intellectual disability participate in work-based learning experiences and determine whether student characteristics (e.g., gender, race/ethnicity, grade level, and support needs) relate to student participation in these activities. An online questionnaire was emailed to high school special education teachers in four states who had a state-issued teaching license in intellectual disability. A total of 266 teachers completed the questionnaire. Descriptive and inferential statistics were used to answer the research questions. Students participated in a variety of work-based learning experiences, with more students participating in school experiences than community experiences. The majority of work experiences were in one of three career clusters: (a) hospitality and tourism; (b) business, management, and administration; or (c) general work skills. Amount of time spent in work experiences varied greatly, with students spending more time in school experiences than community experiences. Student support needs and grade level were related to participation in community experiences. Grade level was also related to the amount of time students spent in school and community experiences.
Postnatal Dynamics and Clinical Associations of Fecal Calprotectin in Very Preterm Infants: Implications for Necrotizing Enterocolitis and Feeding Intolerance
Authors: Hong Luyang , Huang Yihuang , Jiang Siyuan , Han Junyan , Li Shujuan , Zhang Lan , Zhou Qi , Cao Xincheng , Yu Weiyin , Yang Yi , Cao Yun 
Published: June 2023

Introduction:
To elucidate the postnatal dynamics and clinical associations of fecal calprotectin (FC) in very preterm infants, with a focus on necrotizing enterocolitis (NEC) and feeding intolerance (FI).

Methods:
We performed a prospective observational cohort study in infants with a gestational age of <32 weeks or birth weight <1500 g with weekly feces collection. The relationships between FC, NEC, and FI were investigated, adjusting for demographic and clinical factors.

Results:
A total of 1,086 fecal samples were collected from 194 preterm infants. Postnatal FC levels of non-NEC infants were highly variable and followed an age-dependent patterned progression. FC levels were elevated in NEC patients before and at NEC onset, distinguishing them from non-NEC infants and those at sepsis onset. Among infants without NEC or sepsis, those with FI exhibited lower FC concentrations throughout hospitalization and displayed a significant delay in reaching high FC levels after meconium compared to non-FI infants. The age to reach the first high non-meconial FC levels was positively associated with the time to achieve full enteral feeding.

Conclusion:
Postnatal FC dynamics among premature infants followed a patterned progression but were disturbed in patients with NEC and FI. Due to the high variations, the use of FC levels in NEC diagnosis should be implemented with caution in clinical practice. FC may help understand FI and feeding progression in very preterm infants. Further research is needed to validate these findings and explore the potential clinical applications of FC in this population.
Transcutaneous spinal stimulation in people with and without spinal cord injury: Effect of electrode placement and trains of stimulation on threshold intensity
Authors: Finn Harrison T. , Bye Elizabeth A. , Elphick Thomas G. , Boswell‐Ruys Claire L. , Gandevia Simon C. , Butler Jane E. , Héroux Martin E. 
Published: June 2023

AbstractTranscutaneous spinal cord stimulation (TSS) is purported to improve motor function in people after spinal cord injury (SCI). However, several methodology aspects are yet to be explored. We investigated whether stimulation configuration affected the intensity needed to elicit spinally evoked motor responses (sEMR) in four lower limb muscles bilaterally. Also, since stimulation intensity for therapeutic TSS (i.e., trains of stimulation, typically delivered at 15–50 Hz) is sometimes based on the single‐pulse threshold intensity, we compared these two stimulation types. In non‐SCI participants (n = 9) and participants with a SCI (n = 9), three different electrode configurations (cathode–anode); L1‐midline (below the umbilicus), T11‐midline and L1‐ASIS (anterior superior iliac spine; non‐SCI only) were compared for the sEMR threshold intensity using single pulses or trains of stimulation which were recorded in the vastus medialis, medial hamstring, tibialis anterior, medial gastrocnemius muscles. In non‐SCI participants, the L1‐midline configuration showed lower sEMR thresholds compared to T11‐midline (p = 0.002) and L1‐ASIS (p < 0.001). There was no difference between T11‐midline and L1‐midline for participants with SCI (p = 0.245). Spinally evoked motor response thresholds were ~13% lower during trains of stimulation compared to single pulses in non‐SCI participants (p < 0.001), but not in participants with SCI (p = 0.101). With trains of stimulation, threshold intensities were slightly lower and the incidence of sEMR was considerably lower. Overall, stimulation threshold intensities were generally lower with the L1‐midline electrode configuration and is therefore preferred. While single‐pulse threshold intensities may overestimate threshold intensities for therapeutic TSS, tolerance to trains of stimulation will be the limiting factor in most cases.
Curvature of gastrocnemius muscle fascicles as function of muscle–tendon complex length and contraction in humans
Authors: Heieis Jule , Böcker Jonas , D'Angelo Olfa , Mittag Uwe , Albracht Kirsten , Schönau Eckhard , Meyer Andreas , Voigtmann Thomas , Rittweger Jörn 
Published: June 2023

AbstractIt has been shown that muscle fascicle curvature increases with increasing contraction level and decreasing muscle–tendon complex length. The analyses were done with limited examination windows concerning contraction level, muscle–tendon complex length, and/or intramuscular position of ultrasound imaging. With this study we aimed to investigate the correlation between fascicle arching and contraction, muscle–tendon complex length and their associated architectural parameters in gastrocnemius muscles to develop hypotheses concerning the fundamental mechanism of fascicle curving. Twelve participants were tested in five different positions (90°/105°*, 90°/90°*, 135°/90°*, 170°/90°*, and 170°/75°*; *knee/ankle angle). They performed isometric contractions at four different contraction levels (5%, 25%, 50%, and 75% of maximum voluntary contraction) in each position. Panoramic ultrasound images of gastrocnemius muscles were collected at rest and during constant contraction. Aponeuroses and fascicles were tracked in all ultrasound images and the parameters fascicle curvature, muscle–tendon complex strain, contraction level, pennation angle, fascicle length, fascicle strain, intramuscular position, sex and age group were analyzed by linear mixed effect models. Mean fascicle curvature of the medial gastrocnemius increased with contraction level (+5 m−1 from 0% to 100%; p = 0.006). Muscle–tendon complex length had no significant impact on mean fascicle curvature. Mean pennation angle (2.2 m−1 per 10°; p < 0.001), inverse mean fascicle length (20 m−1 per cm−1; p = 0.003), and mean fascicle strain (−0.07 m−1 per +10%; p = 0.004) correlated with mean fascicle curvature. Evidence has also been found for intermuscular, intramuscular, and sex‐specific intramuscular differences of fascicle curving. Pennation angle and the inverse fascicle length show the highest predictive capacities for fascicle curving. Due to the strong correlations between pennation angle and fascicle curvature and the intramuscular pattern of curving we suggest for future studies to examine correlations between fascicle curvature and intramuscular fluid pressure.
Characterization of Hofbauer cell polarization and VEGF localization in human term placenta from active and inactive pregnant individuals
Authors: Goudreau Alexandra D. , Everest Catherine , Tanara Layli , Tzaneva Velislava , Adamo Kristi B. 
Published: June 2023

AbstractPhysical activity (PA) during pregnancy is associated with parental and fetal health benefits; however, the mechanisms through which these benefits arise are yet to be fully understood. In healthy pregnancies Hofbauer cells (HBCs) comprise a heterogenous population containing CD206+ and CD206− phenotypes. In healthy pregnancies, CD206+ represent the majority, while dysregulations have been associated with pathological conditions. HBCs have also been identified as potential drivers of angiogenesis. As PA induces changes in macrophage polarization in non‐pregnant populations, this novel study examined the relationship between PA and HBC polarization and to identify which HBC phenotypes express VEGF. Participants were classified as active or inactive, and immunofluorescence cell‐labelling was used to quantify total HBCs, CD206+ HBCs, and the proportion of total HBCs expressing CD206. Immunofluorescent colocalization assessed which phenotypes expressed VEGF. Protein and mRNA expression of CD68 and CD206 were measured in term placenta tissue using Western blot and RT‐qPCR, respectively. Both CD206+ and CD206− HBCs expressed VEGF. The proportion of CD206+ HBCs was elevated in active individuals; however, CD206 protein expression was observed to be lower in active participants. Combined with a lack of significant differences in CD206 mRNA levels, these findings suggest potential PA‐mediated responses in HBC polarization and CD206 translational regulation.
Exploring Tibetan residents’ everyday language practices in Danba county, Southwest China: a case study
Authors: Ai Bin , Ma Siwei , Liu Xia 
Published: June 2023

Abstract
In this case study, seventy Tibetan residents selected from two Tibetan villages in Danba county, Southwest China, were invited to participate in an investigation of their everyday use of Tibetan, standard Chinese, and English, as well as their expectations of future multilingual practices. It was found that there are intergenerational differences in the participants’ use of these languages. This paper, in the context of neoliberal ideology and rural development, suggests that attention should be given to support local Tibetan residents’ language learning and improve their multilingual competence, thus enabling the conversion of their linguistic/human capital to economic capital. The study contributes to understandings of how local multilingual resources can be exploited for the betterment of living standards and opportunities, and it calls for governments to address the issues regarding poverty reduction, rural development, and language preservation by improving the quality of multilingual education in ethnic minority areas.
Microstructural evolution and dynamic recrystallization model of extruded homogenized AZ31 magnesium alloy during hot deformation
Authors: Yan Zehua , Zhou Jingkai , Zhang Wei 
Published: June 2023

Abstract
Using a thermal simulation testing machine, hot compression experiments were carried out on extruded homogenized AZ31 magnesium alloy, and the hot deformation behavior was analyzed. Based on this, the constitutive equation of the alloy is constructed to explore the evolution law of microstructure during hot deformation of the alloy, which could provide theoretical guidance for the reasonable selection of parameter ranges during hot compression of extruded homogenized AZ31 magnesium alloy. The experimental result indicated that the flow stress of the alloy during the hot deformation decreases with increasing temperature, increases with the strain rate increasing, and the real stress–strain curves during deformation show dynamic recrystallization curves. According to the experimental results, the deformation activation energy Q is 126.882 kJ mol−1 and the stress exponent n is 4.36 calculated by the constitutive equation under the given parameters, which confirmed that the glide and climb of dislocations in the climb-controlled regime is the deformation mechanism in this work. Decreasing the compression temperature and increasing the strain rate are helpful to reduce the Zener–Hollomon parameter, control the dynamic recrystallization occurring, and refine grain size to improve the mechanical properties effectively. Moreover, the dynamic recrystallization model of the alloy was constructed using the work hardening rate method and regression method, including dynamic recrystallization critical condition model, dynamic model and grain size model.
Revisiting areal and lexical diffusion: the case of Viennese Monophthongization in Austria’s traditional dialects
Authors: Vergeiner Philip C. , Luttenberger Jan , Bülow Lars , Wallner Dominik , Britain David 
Published: June 2023

AbstractThis paper investigates the geographical and structural diffusion of “Viennese Monophthongization” (VM). By means of a new numerical measure to assess and compare formant movement in 18 lexical items, we provide evidence that VM is an ongoing, regular sound change transforming [aɛ̯] and [aɔ̯] gradually into [æː] and [ɒː]. Data are based on direct dialect recordings of 76 speakers in two age groups in 19 eastern and central Austrian rural locations. Results indicate that VM is diffusing from Vienna in a wave-like fashion. Even though VM is reported to have been established in other bigger cities for more than 30 years, the data show no evidence of diffusion from these cities. There are also other factors affecting the degree of formant movement: The phonetic-phonological environment (stress and the following consonant) explains most of the variance in the data, whereas no frequency effects could be found. Social identity, cultural space, and gender-related network structures are discussed as additional social factors.
Circular RNA Circ_0038467 promotes the maturation of miRNA-203 to increase lipopolysaccharide-induced apoptosis of chondrocytes
Authors: Gou Zhongkun , Wu Quanling , Jiang Changqing , Dong Wei 
Published: June 2023

Abstract
Circ_0038467 and miR-203 exert important functions in lipopolysaccharide (LPS)-induced inflammation, which contributes to osteoarthritis (OA). Our preliminary deep sequencing analysis revealed altered expression of Circ_0038467 and miR-203 in OA and a close correlation between them. This study was therefore to explore crosstalk between them in OA. The expression of Circ_0038467, mature miR-203, and miR-203 precursor in OA patients and controls was determined using RT-qPCR. An overexpression assay was performed to explore the role of Circ_0038467 in regulating the expression of mature miR-203 and miR-203 precursor. Cell apoptosis was analyzed by cell apoptosis assay. Circ_0038467 was upregulated in OA and positively correlated with mature miR-203 but not that of miR-203 precursor. In chondrocytes, increased expression levels of both Circ_0038467 and miR-203 were observed after LPS treatment. In chondrocytes, overexpression of Circ_0038467 increased the expression levels of mature miR-203 but not that of miR-203 precursor. Overexpression of Circ_0038467 and miR-203 increased cell apoptosis. Then, the miR-203 inhibitor reversed the effects of overexpression of Circ_0038467 on cell apoptosis. Interestingly, Circ_0038467 was detected in both the cytoplasm and nucleus. Circ_0038467 directly interacted with the precursor miR-203. Therefore, Circ_0038467 is highly expressed in OA and it may promote the production of mature miR-203 to increase apoptosis of chondrocytes induced by LPS.