bluuebunny/crossref_metadata_2025_split huggingface dataset:
Liquid Manipulator with Printed Electrode Patterns for Soft Robotic Systems
Authors: Zhu Pingan , Tang Wei , Jiao Zhongdong , Xu Huxiu , Hu Yu , Qu Yang , Yang Huayong , Zou Jun 
Published: April 2023

AbstractSoft robots are able to safely operate in a dynamically evolving or highly unstructured environment by virtue of their inherited compliance. Among the available actuation methods for soft robotics, fluid‐driven is frequently preferred due to holistic design considerations that enable multipurpose yet straightforward mechanical solutions to complicated issues. The motion of a soft fluid‐driven robotic system usually relies on the flow of its internal fluid, however, existing fluid pumps only enable a single linear flow, which severely hinders the integrated multifunctional design of soft robots. Herein, a class of liquid manipulator is proposed based on electrohydrodynamic (EHD) effect, which consists of a flexible substrate with a printed planar electrode pattern. By streamlining the electric field distribution via electrode pattern, the liquid manipulator can manipulate the fluid flow, allowing fluid to perform multiple movement behaviors, including tangential, radial, and linear motions. These liquid manipulators are fabricated by printing planar electrode patterns onto flexible substrates using the digital printing method, in which both the printed electrodes and substrate are flexible and bendable. These liquid manipulators are flexible, fast‐response, thin, lightweight, and scalable, which can be easily embedded into soft fluidic systems with insignificant footprint and weight and actuate their rapid movements. Three liquid manipulators are embedded into a continuously rotating motor, focus‐tunable liquid lens, and soft linear actuator to achieve their multiple motions, i.e., continuous rotation, radial motion, and linear motion, illustrating broad potential of liquid manipulators in soft robotic systems.
Mixed Diethanolamine and Polyethyleneimine with Enhanced CO2 Capture Capacity from Air
Authors: Miao Yihe , Wang Yaozu , Ge Bingyao , He Zhijun , Zhu Xuancan , Li Jia , Liu Shanke , Yu Lijun 
Published: April 2023

AbstractSupported polyethyleneimine (PEI) adsorbent is one of the most promising commercial direct air capture (DAC) adsorbents with a long research history since 2002. Although great efforts have been input, there are still limited improvements for this material in its CO2 capacity and adsorption kinetics under ultradilute conditions. Supported PEI also suffers significantly reduced adsorption capacities when working at sub‐ambient temperatures. This study reports that mixing diethanolamine (DEA) into supported PEI can increase 46% and 176% of pseudoequilibrium CO2 capacities at DAC conditions compared to the supported PEI and DEA, respectively. The mixed DEA/PEI functionalized adsorbents maintain the adsorption capacity at sub‐ambient temperatures of −5 to 25 °C. In comparison, a 55% reduction of CO2 capacity is observed for supported PEI when the operating temperature decreases from 25 to −5 °C. In addition, the supported mixed DEA/PEI with a ratio of 1:1 also shows fast desorption kinetics at temperatures as low as 70 °C, resulting in maintaining high thermal and chemical stability over 50 DAC cycles with a high average CO2 working capacity of 1.29 mmol g−1. These findings suggest that the concept of “mixed amine”, widely studied in the solvent system, is also practical to supported amine for DAC applications.
Expanding the phenotype of Seckel syndrome associated with biallelic loss‐of‐function variants in CEP63
Authors: Pekkola Pacheco Nadja , Pettersson Maria , Lindstrand Anna , Grigelioniene Giedre 
Published: April 2023

AbstractSeckel syndrome is an ultrarare autosomal recessive genetically heterogenous condition characterized by intrauterine and postnatal growth restriction, proportionate severe short stature, severe microcephaly, intellectual disability, and distinctive facial features including a prominent nose. Up to now, 40 patients with molecularly confirmed Seckel syndrome have been reported with biallelic variants in nine genes: ATR, CENPJ, CEP63, CEP152, DNA2, NIN, NSMCE2, RBBP8, and TRAIP. Homozygosity for nonsense variant (c.129G>A, p.43*) in CEP63 was described in three cousins with microcephaly, short stature, mild to moderate intellectual disability and diagnoses of Seckel syndrome. Here, we report a second family with three siblings who are compound heterozygous for loss‐of‐function variants in CEP63, c.1125T>G, p.(Tyr375*) and c.595del, p.(Glu199Asnfs*11). All siblings present with microcephaly, prominent nose, and intellectual disability but only one has severe short stature. Two siblings have aggressive behavior, a feature previously not reported in Seckel syndrome. This report adds two novel truncating variants in CEP63 and extends the clinical knowledge on CEP63‐related conditions.
Isobaric Stable Isotope N‐Phosphorylation Labeling (iSIPL) for Ultrasensitive Proteome Quantification
Authors: Wang Xiao‐Yu , Chen Chun‐Jing , He Yao‐Hui , Ding Lian‐Shuai , Wu Yi‐Fan , Huang Cheng‐Ting , Wu Jun , Ding Rong , Xue Yu‐Hua , Lin Zhi‐Wei , Xu Peng‐Xiang , Wu Yi‐Le , Liu Wen , Li Ji‐Jun , Chen Si‐Ming , Zhao Yu‐Fen , Dong Ji‐Yang , Zhou Qiang , Gao Xiang 
Published: April 2023

AbstractStable isotope chemical labeling methods have been widely used for high‐throughput mass spectrometry (MS)‐based quantitative proteomics in biological and clinical applications. However, the existing methods are far from meeting the requirements for high sensitivity detection. In the present study, a novel isobaric stable isotope N‐phosphorylation labeling (iSIPL) strategy was developed for quantitative proteome analysis. The tryptic peptides were selectively labeled with iSIPL tag to generate the novel reporter ions containing phosphoramidate P−N bond with high intensities under lower collision energies. iSIPL strategy are suitable for peptide sequencing and quantitative analysis with high sensitivity and accuracy even for samples of limited quantity. Furthermore, iSIPL coupled with affinity purification and mass spectrometry was applied to measure the dynamics of cyclin dependent kinase 9 (CDK9) interactomes during transactivation of the HIV‐1 provirus. The interaction of CDK9 with PARP13 was found to significantly decrease during Tat‐induced activation of HIV‐1 gene transcription, suggesting the effectiveness of iSIPL strategy in dynamic analysis of protein‐protein interaction in vivo. More than that, the proposed iSIPL strategy would facilitate large‐scale accurate quantitative proteomics by increasing multiplexing capability.
Endocranial anatomy of the early prozostrodonts (Eucynodontia: Probainognathia) and the neurosensory evolution in mammal forerunners
Authors: Kerber Leonardo , Roese‐Miron Lívia , Bubadué Jamile M. , Martinelli Agustín G. 
Published: April 2023

AbstractProzostrodon brasiliensis and Therioherpeton cargnini are non‐mammaliaform cynodonts that lived ~233 million years ago (late Carnian, Late Triassic) in western Gondwana. They represent some of the earliest divergent members of the clade Prozostrodontia, which includes “tritheledontids”, tritylodontids, “brasilodontids”, and mammaliaforms (including Mammalia as crown group). Here, we studied the endocranial anatomy (cranial endocast, nerves, vessels, ducts, ear region, and nasal cavity) of these two species. Our findings suggest that during the Carnian, early prozostrodonts had a brain with well‐developed olfactory bulbs, expanded cerebral hemispheres divided by the interhemispheric sulcus, and absence of an unossified zone and pineal body. The morphology of the maxillary canal represents the necessary condition for the presence of facial vibrissae. A slight decrease in encephalization is observed at the origin of the clade Prozostrodontia. This new anatomical information provides evidence for the evolution of endocranial traits of the first prozotrodonts, a Late Triassic lineage that culminated in the origin of mammals.
The Conservation Potential of Smart Irrigation Controllers for Over‐Irrigators
Authors: Taylor Nicholas , Atkinson Connor , Price Kaitlin Robb , Johnson Parker , Spatz Bradley 
Published: April 2023

Key TakeawaysWith water demand projected to rise substantially in Florida, strategically designed and targeted conservation programs are important for reducing overall water use.According to a Florida study, over‐irrigators could cut their monthly water bills by more than $35 by using a smart irrigation controller.Utilities stand to benefit from establishing effective conservation programs; in the case of this study, water demand could drop by 1.62 mgd if smart irrigation controller rebates are targeted to over‐irrigators.
Ribosomal protein L32 enhances hepatocellular carcinoma progression
Authors: Hou Guoxin , Lu Zhimin , Jiang Jialu , Yang Xinmei 
Published: April 2023

AbstractPurposeThe underlying mechanisms of hepatocellular carcinoma (HCC) have not been fully investigated, and effective biomarkers for HCC are still needed to be explored. Therefore, our study sought to thoroughly examine the clinical significance and biological functions of the ribosomal protein L32 (RPL32) in HCC by coupling bioinformatic methods with experimental analysis.MethodsTo determine the clinical significance of RPL32, bioinformatic analyses were performed to examine RPL32 expression in HCC patient samples and to correlate RPL32 expression and HCC patient survival rates, genetic alterations, and immune cell infiltration. Cell counting kit‐8 assays, colony formation, flow cytometry, and transwell assays were performed to examine the effects of RPL32 on HCC cell proliferation, apoptosis, migration, and invasion in HCC cell lines (SMMC‐7721 and SK‐HEP‐1) where RPL32 was silenced using small interfering ribonucleic acid.ResultsIn the current study, we show that RPL32 was highly expressed in HCC samples. Moreover, high levels of RPL32 were associated with unfavorable outcomes in patients with HCC. Promoter methylation and copy number variation of RPL32 were associated with RPL32 mRNA expression. Results from the RPL32 silencing experiments indicated that the proliferation, apoptosis, migration, and invasion of SMMC‐7721 and SK‐HEP‐1 cells were attenuated upon RPL32 depletion.ConclusionRPL32 correlates with a favorable prognosis in patients with HCC and promotes the survival, migration, and invasion of HCC cells.
Comparative analysis of adjuvant therapy for stage III BRAF‐mut melanoma: A real‐world retrospective study from single center in China
Authors: Zhong Jingqin , Sun Wei , Hu Tu , Wang Chunmeng , Yan Wangjun , Luo Zhiguo , Liu Xin , Xu Yu , Chen Yong 
Published: April 2023

AbstractBackgroundBRAF V600 mutation is the most common oncogenic alternation in melanoma and is visible in around 50% of cutaneous and 10%–15% of acral or mucosal subtypes. Currently, immunotherapy with anti‐PD‐1 blockade and dual‐targeted therapy with Dabrafenib plus trametinib (D + T) target therapy have been approved as adjuvant therapies for Stage III melanoma with BRAF V600 mutation. According to their phase III clinical trials, 3‐year recurrence‐free survival (RFS) is around 60% for both types of treatment. However, early disease control was slightly more effective with targeted therapy than immunotherapy. With different drug approval deadlines in China, anti‐PD1 monotherapy, D + T combination, and Vemurafenib (V) monotherapy have all been used in real clinical practice as adjuvant settings for stage III BRAF‐mut melanoma in recent years. We conducted this retrospective study to evaluate the efficacy of different treatments in the Chinese melanoma population.MethodsPatients who underwent radical surgery and were diagnosed as Stage III melanoma harboring BRAF V600 mutation by pathological report were retrospectively identified at Fudan University Shanghai Cancer Center from January 2017 to December 2021. Patients with mucosal melanoma, or with follow‐up of <6 months, or receiving other adjuvant treatment were excluded. Pearson's chi‐squared test or Fisher's exact test was performed for univariable analysis of the different adjuvant groups. Log‐rank analysis was used to identify prognostic factors for relapse‐free survival (RFS).ResultsNinety‐three patients with resected stage III melanoma with BRAF V600E mutation were identified in our study, including 25 patients receiving adjuvant anti‐PD‐1 immunotherapy (PD‐1), 25 receiving adjuvant D + T, 23 receiving V, and 20 patients with observation‐only (OBS). There were no statistical differences between treatment groups in baseline characteristics including age, gender, subtypes, primary thickness, ulceration, and nodal involvement. Median relapse‐free survival (RFS) time was not reached in the D + T group, 15 months in the V group, 15 months in the PD‐1 group, and 10 months in the OBS group, respectively. Compared to OBS, all three other groups showed a tendency to benefit from RFS, while only D + T achieved a statistical difference (p = 0.002). However, compared to D + T, anti‐PD‐1 monotherapy also showed significantly worse relapse control (p = 0.032).ConclusionsFor Chinese stage III melanoma with BRAF mutation, both novel targeted therapy and immunotherapy showed potential benefits in relapse‐free survival compared to observation only. Dual‐targeted D + T therapy may still be the best choice for adjuvant therapy because anti‐PD‐1 monotherapy has failed to report equivalent efficacy in real‐world practice.
The impact of psychological distress and university counselling on academic outcomes: Analysis of a routine practice‐based dataset
Authors: Scruggs Robert , Broglia Emma , Barkham Michael , Duncan Charlie 
Published: April 2023

AbstractWhole university approaches to student mental health and well‐being increasingly involve university counselling and mental health services (UCMHSs) as key stakeholders in higher education and the fulfilment of good academic outcomes. However, previous research using routine outcome measures has focussed on psychological distress only. Research is needed to demonstrate the value of university counselling on academic outcomes. This study aimed at profiling the psychological distress of a student sample according to the Clinical Outcomes in Routine Evaluation—Outcome Measure (CORE‐OM); measuring the change in perceived impact of problems on academic outcomes, and measuring the perceived impact of counselling on academic outcomes. Students from two UK university counselling services completed the CORE‐OM and the Counselling Impact on Academic Outcomes (CIAO) questionnaire as part of routine practice. After counselling, 67.4% (n = 323) of students with planned endings to counselling showed at least reliable improvement on the CORE‐OM. Significant reductions in the perceived impact of problems on all academic outcomes were also found. On average, 83% (n = 398) of students found counselling helpful for academic outcomes to at least a limited extent. University counselling was found to reduce psychological distress and the impact of problems on academic outcomes. Psychometric examination of the CIAO tool is warranted to strengthen its use. The need for robust data across UCMHSs is demonstrated by both the strengths and limitations of this study.
Recent Progress on Cyclic Peptides’ Assembly and Biomedical Applications
Authors: Wu Chongyang , Wang Huaimin 
Published: April 2023

AbstractCyclic peptides are important building blocks for forming functional structures and have been applied in various fields. Considering the significant structural and functional roles of cyclic peptides in materials science and the attributed biophysical advantages, we provide an overview of cyclic peptide types that can self‐assemble to form nanotubes, recent progress in stimuli‐triggered cyclic peptide assembly, and methods to construct peptide and polymer conjugates based on cyclic peptides with alternative chirality. Specifically, we highlight the roles that stimuli‐triggered cyclic peptides and their conjugates play in biomedical applications. Recent progress in other cyclic peptides acting as gelators in drug delivery and biomedicine are also summarized. These cyclic peptides with self‐assembly properties are expected to act as adaptive systems for drug delivery and selective disease targeting.
Biophysical and Computational Approaches to Study Ternary Complexes: A ‘Cooperative Relationship’ to Rationalize Targeted Protein Degradation
Authors: Ward Jake A. , Perez‐Lopez Carles , Mayor‐Ruiz Cristina 
Published: April 2023

AbstractDegraders have illustrated that compound‐induced proximity to E3 ubiquitin ligases can prompt the ubiquitination and degradation of disease‐relevant proteins. Hence, this pharmacology is becoming a promising alternative and complement to available therapeutic interventions (e. g., inhibitors). Degraders rely on protein binding instead of inhibition and, hence, they hold the promise to broaden the druggable proteome. Biophysical and structural biology approaches have been the cornerstone of understanding and rationalizing degrader‐induced ternary complex formation. Computational models have now started to harness the experimental data from these approaches with the aim to identify and rationally help design new degraders. This review outlines the current experimental and computational strategies used to study ternary complex formation and degradation and highlights the importance of effective crosstalk between these approaches in the advancement of the targeted protein degradation (TPD) field. As our understanding of the molecular features that govern drug‐induced interactions grows, faster optimizations and superior therapeutic innovations for TPD and other proximity‐inducing modalities are sure to follow.
Toward Rational Design of Carbon Nanodots with High Photothermal Efficiency for Tumor Photothermal Therapy†
Authors: Liu Enshan , Wu Jun , Liang Tao , Zhang Bohan , Tang Zikang , Qu Songnan 
Published: April 2023

Comprehensive SummaryCarbon nanodots (CDs) with high photothermal performance are ideal candidates for tumor photothermal therapy (PTT). Herein, we investigated the photothermal performance of CDs synthesized from urea and citric acid via solvothermal method in dimethyl sulfoxide (DMSO) at different temperatures. Photothermal conversion efficiency (PCE) of up to 61.3% was obtained in the CDs synthesized at 150 °C (150‐CDs), which is much better than the CDs synthesized at 180 °C (180‐CDs). By analyzing the morphologies, chemical structures, and absorption spectra of these CDs, we found that the photothermal effect of the CDs was due to the lattice vibration of their carbonized cores, in which moderate carbonization with high content of surface carbonyl group (C=O) contributed for enhanced absorption band in deep red region with improved photothermal performance. Moreover, we also demonstrated that 150‐CDs with moderate carbonized cores exhibit much better tumor PTT performance in vivo than 180‐CDs with higher carbonized cores.
Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications
Authors: Kümmerlen Katja , Raspe Sophie , Harzsch Steffen 
Published: April 2023

AbstractIn mandibulate arthropods, the primary olfactory centers, termed olfactory lobes in crustaceans, are typically organized in distinct fields of dense synaptic neuropils called olfactory glomeruli. In addition to olfactory sensory neuron terminals and their postsynaptic efferents, the glomeruli are innervated by diverse neurochemically distinctive interneurons. The functional morphology of the olfactory glomeruli is understudied in crustaceans compared with insects and even less well understood and described in a particular crustacean subgroup, the Peracarida, which embrace, for example, Amphipoda and Isopoda. Using immunohistochemistry combined with confocal laser scanning microscopy, we analyzed the neurochemistry of the olfactory pathway in the amphipod Parhyale hawaiensis. We localized the biogenic amines serotonin and histamine as well as the neuropeptides RFamide, allatostatin, orcokinin, and SIFamide. As for other classical neurotransmitters, we stained for γ‐aminobutyric acid and glutamate decarboxylase and used choline acetyltransferase as indicator for acetylcholine. Our study is another step in understanding principles of olfactory processing in crustaceans and can serve as a basis for understanding evolutionary transformations of crustacean olfactory systems.
Improved aquila optimization‐based parameter evaluation framework and meta‐heuristic algorithm in radio‐over‐fiber SpatialMux multi‐input multi‐output long‐term evolution system
Authors: Rather Irfan Ahmad , Kumar Gulshan , Saha Rahul 
Published: April 2023

SummaryIn heterogeneous access network, Multiple‐Input Multiple‐Output (MIMO) radio‐over‐fiber (RoF) system is an efficient approach for multiple signal transmission with low cost and complexity. The performance of RoF fronthaul system in MIMO system will be varied with different nonlinear effects. By adjusting various transmission parameters, such as the input signal power or the laser bias current, the nonlinear impacts produced by the RoF system can be reduced. In this paper, a novel algorithm Improved Aquila Optimization (IAO) is proposed to optimize transmission circumstances of MIMO RoF system. It determines the appropriate bias current for both lasers and Radio Frequency (RF) signal power in a short period. The input signals are wavelength multiplexed with Intensity Modulation and Direct Detection (IM/DD) applied. The carrier as well as transmission frequency is governed by the MIMO‐Long‐Term Evolution (LTE) standard. The proposed system is implemented in MATLAB, and the performance is evaluated. The experimental results show that fast convergence and trade‐off between noise and nonlinearity are obtained with varying bandwidth. In the experimental scenario, the maximum Error Vector Magnitude (EVM) of 1.88, 3.14, and signal‐to‐noise ratio (SNR) of 3.204, and 2.698 was attained for both quadrature phase shift keying (QPSK) and quadrature amplitude modulation (QAM) modulation. [Correction added on 24 April 2023, after first online publication: the SNR values were corrected in the preceding sentence.] For 100 iterations, the processing time was reduced to 0.137 s. When compared with the conventional state‐of‐the‐art approaches, the accuracy and computational complexity of the proposed approach are improved.
Study on nutritional components evaluation and origin differences of Agrocybe cylindracea from different regions
Authors: Chen Lisha , Chen Yu , Wang Jiang , Wang Zhiqiang , Huang Xiaojun 
Published: April 2023

AbstractThe aim of this study is to investigate the nutrients of Agrocybe cylindracea from different regions and discover the law of its nutritional value. In this study, 43 A. cylindracea samples were collected from 13 provinces in China. The differences in nutritional components of this A. cylindracea were explored. The main nutritional components, including protein, dietary fiber, amino acids, fat, ash, and moisture, were evaluated. Further factor and cluster analysis were applied to analyze these nutrients. The results showed A. cylindracea had high protein (14.09–27.24 g/100 g dw) and insoluble dietary fiber (25.27–34.17 g/100 g dw), but low soluble dietary fiber (0.49–3.19 g/100 g dw) and fat (1.69–3.45 g/100 g dw). Seventeen amino acids were detected from these mushrooms, of which glutamic (18.24–46.78 g/100 g dw) was the most abundant. Based on Eta analysis, factor analysis, and cluster analysis, it was found that the comprehensive nutritional value of A. cylindracea was related to the factors of both the geographical origin and maturity stage. In addition, geographical origin had a greater influence on the nutritional value of A. cylindracea than the maturity stage. This study will serve as a useful reference for the selection of the consumption and further processing directions of A. cylindracea.