bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induction of epithelial-mesenchymal transition in thyroid follicular cells is associated with cell adhesion alterations and low-dose hyper-radiosensitivity
Authors: Mathur Ankit , Chinnadurai Vijayakumar , Bhalla Param Jit Singh , Chandna Sudhir 
Published: September 2023

BACKGROUND: Epithelial-mesenchymal transition (EMT) is associated with altered cellular adhesion. We previously demonstrated that cellular adhesion influences Low-dose Hyper-Radiosensitivity (HRS) in a variety of tumor cells. However, the relationship of low-dose HRS with the phenotypic plasticity incurred by EMT during the neoplastic transformation remains to be elucidated. OBJECTIVE: To investigate whether acquisition of EMT phenotype during progressive neoplastic transformation may affect low-dose radiation sensitivity. METHODS: Primary thyroid cells obtained from a human cystic thyroid nodule were first subjected to nutritional stress. This yielded immortalized INM-Thy1 cell strain, which was further treated with either multiple γ-radiation fractions (1.5 Gy each) or repetitive cycles of 3-methylcholanthrene and phorbol-12-myristate-13-acetate, yielding two progressive transformants, viz., INM-Thy1R and INM-Thy1C. Morphological alterations, chromosomal double-minutes, cell adhesion proteins, anchorage dependency, tumorigenicity in nude mice and cellular radiosensitivity were studied in these strains. RESULTS: Both transformants (INM-Thy1R, INM-Thy1C) displayed progressive tumorigenic features, viz., soft agar colony growth and solid tumor growth in nude mice, coupled with features of epithelial-mesenchymal transition and activated Wnt pathway. Incidentally, the chemical-induced transformant (INM-Thy1C) displayed a prominent HRS (αs/αr = 29.35) which remained unaffected at high cell density. However, the parental (INM-Thy1) cell line as well as radiation-induced transformant (INM-Thy1R) failed to show this hypersensitivity. CONCLUSION: The study shows that induction of EMT in thyroid follicular cells may accompany increased susceptibility to low-dose ionizing radiation, which was attenuated by adaptive resistance acquired during radiation-induced transformation.
A densely connected LDCT image denoising network based on dual-edge extraction and multi-scale attention under compound loss
Authors: Lina Jia , Xu He , Aimin Huang , Beibei Jia , Zhiguo Gui 
Published: September 2023

BACKGROUND: Low dose computed tomography (LDCT) uses lower radiation dose, but the reconstructed images contain higher noise that can have negative impact in disease diagnosis. Although deep learning with the edge extraction operators reserves edge information well, only applying the edge extraction operators to input LDCT images does not yield overall satisfactory results. OBJECTIVE: To improve LDCT images quality, this study proposes and tests a dual edge extraction multi-scale attention mechanism convolution neural network (DEMACNN) based on a compound loss. METHODS: The network uses edge extraction operators to extract edge information from both the input images and the feature maps in the network, improving the utilization of the edge operators and retaining the images edge information. The feature enhancement block is constructed by fusing the attention mechanism and multi-scale module, enhancing effective information, while suppressing useless information. The residual learning method is used to learn the network, improving the performance of the network, and solving the problem of gradient disappearance. Except for the network structure, a compound loss function, which consists of the MSE loss, the proposed joint total variation loss, and the edge loss, is proposed to enhance the denoising ability of the network and reserve the edge of images. RESULTS: Compared with other advanced methods (REDCNN, CT-former and EDCNN), the proposed new network achieves the best PSNR and SSIM values in LDCT images of the abdomen, which are 33.3486 and 0.9104, respectively. In addition, the new network also performs well on head and chest image data. CONCLUSION: The experimental results demonstrate that the proposed new network structure and denoising algorithm not only effectively removes the noise in LDCT images, but also protects the edges and details of the images well.
MANAJEMEN INFEKSI RABIES BERISIKO TINGGI: STUDI KASUS TANPA SERUM ANTI-RABIES
Authors: Ambarwaty Mahdika , B. Nara Mario , Theodorus Defranky , Ambarsarie Riry 
Published: September 2023

AbstrakRabies merupakan masalah kesehatan masyarakat yang cukup serius terutama di daerah dan telah masuk dalam program daerah namun terabaikan selama masa pandemi. Seorang anak laki laki berusia empat tahun dibawa ke RSUD TC Hillers Maumere datang dengan keluhan demam dan mual muntah lebih dari lima kali. Dari anamnesis melalui keluarganya diketahui bahwa pasien memiliki riwayat digigit anjing di area wajah dan pergelangan tangan kanan pada 14 hari sebelum muncul keluhan. Pada kasus ini pasien sudah mendapatkan perawatan luka dan Vaksin Anti Rabies (VAR) dosis kedua tetapi tidak mendapatkan Serum Anti Rabies (SAR). Pada kasus dengan area gigitan resiko tinggi, pemberian VAR harus disertai pemberian SAR untuk mengurangi moralitas. Kata kunci: rabies, serum anti-rabies, anakAbstract Rabies is a serious public health problem, especially in the regions and has been included in regional programs but neglected during the pandemic. A four-year-old boy taken to TC Hillers Maumere Hospital came in complaining of fever and nausea, vomiting more than five times. From the history through his family, it is known that the patient had a history of being bitten by a dog in the face and right wrist area in the 14 days before complaints appeared. In this case, the patient has received wound care and the second dose of Anti-Rabies Vaccine (VAR) but did not get Anti-Rabies Serum (SAR). In cases with high-risk bite areas, VAR should be accompanied by SAR to reduce mortality. Keywords: rabies, anti-rabies serum, child
The Impacts of Fecal Microbiota Transplantation from Same Sex on the Symptoms of Ulcerative Colitis Patients
Authors: Shang Shu , Zhu Jian , Liu Xi , Wang Wei , Dai Tingting , Wang Li , Li Baojun 
Published: September 2023

Abstract
We aimed to compare the clinical efficacy of fecal microbiota transplantation (FMT) from the same sex on ulcerative colitis (UC) patients. A total of 272 UC patients were selected in the prospective clinical study, which incorporated four distinct groups, each comprising male and female patients, who were either receiving FMT or placebo, respectively. FMT was performed by sending the gut microbiota of healthy female or male adolescents to the same gender patients via gastroscope three times (one time/three weeks), and a placebo was used with an equal volume of saline. Abdominal pain, diarrhea, thick bloody stool, intestinal mucosal lesion, and Mayo scores were measured. Self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were evaluated. The changes of intestinal flora were detected by the 16S rRNA sequencing. FMT reduced the scores of diarrhea, abdominal pain, mucosal lesion, and Mayo, SAS, and SDS in UC patients compared to the placebo group (p < 0.05). Clostridiales and Desulfovibrionaceae were dominant in gut microbiota from male patients and were reduced after FMT. Meanwhile, the abundance of Prevotella, Lactobacillus, and Bifidobacterium was increased in the male group. Female patients had a higher abundance of Escherichia-Shigella, Desulfovibrionaceae, and Staphylococcaceae before FMT, and it was reduced after FMT. Meanwhile, the abundance of Porphyromonadaceae, Prevotella, Lactobacillus, and Bifidobacterium was increased in the female group. There were no significant changes for the species in the corresponding placebo groups. FMT improved the UC symptoms of male and female patients, which may be associated with different gut microbiota changes.
Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from Arthrobacter sp. CSAJ-16 in Cangshan Mountain
Authors: Yan Xin , Hu Wei , Zhu Yun-Guo , Liu Qing-Qing , Wang Shuai , Liu Hong-Yan , Zhu Dan , Lv Zhi-Hua , Li Lin-Hua , Yin Yi-Rui 
Published: September 2023

Abstract
Uricase (or Urate oxidase), a key enzyme involved in purine metabolism, is commonly used in treating conditions such as gout, hyperuricemia, and tumor lysis syndrome. In this study, a uricase-producing strain (named CSAJ-16) was isolated from the soil sample of Cangshan Mountain, Yunnan Province, China. This strain was identified as Arthrobacter sp. CSAJ-16. Based on the gene sequence alignment, the uricase gene (named aruox) of Arthrobacter sp. CSAJ-16 was amplified and heterologously expressed. The recombinant uricase (ArUOX) was about 32 kDa. The optimal pH and temperature of ArUOX were pH 7 and 20°C, respectively. The ArUOX remained above 50% relative activity after incubation at 37°C for 100 min or at pH 6.0–8.6 for 24 h. Moreover, metal ions such as K+, Mg2+, Ca2+, Ba2+ and Pb2+ can significantly enhance the activity of ArUOX (> 200%). These enzymatic properties indicate that ArUOX has potential applications in pharmaceutical enzymes and uric acid detection kits.
Screening of Potential Probiotic Lactobacillaceae and Their Improvement of Type 2 Diabetes Mellitus by Promoting PI3K/AKT Signaling Pathway in db/db Mice
Authors: Li Yueyang , Tong Tong , Li Peifan , Peng Yian , Zhang Michael , Liu Jia , She Yongxin , Li Zuming , Li Yongli 
Published: September 2023

Abstract
The study aimed to isolate Lactobacillaceae strains with in vitro hypoglycemic activity and probiotic properties and to determine their antidiabetic abilities in vivo. Lactiplantibacillus plantarum 22, L. plantarum 25, Limosilactobacillus fermentum 11, and L. fermentum 305 with high in vitro hypoglycemic activity were screened from 23 strains of Lactobacillaceae isolated from human feces and identified by 16S rDNA sequencing. The fasting blood glucose (FBG) of the mice was recorded weekly. After 12 weeks, liver, kidney, and pancreas tissues were stained with hematoxylin and eosin (H&E) to observe histomorphology; the inflammatory factors were assayed by Quantitative Real-time PCR; PI3K and AKT were measured by Western blot; the short-chain fatty acids (SCFAs) were determined by LC-MS/MS. Inhibitory activities of L. plantarum 22, L. plantarum 25, L. fermentum 11, and L. fermentum 305 against α-amylase were 62.29 ± 0.44%, 51.81 ± 3.65%, 58.40 ± 1.68%, and 57.48 ± 5.04%, respectively. Their inhibitory activities to α-glucosidase were 14.89 ± 0.38%, 15.32 ± 0.89%, 52.63 ± 3.07%, and 51.79 ± 1.13%, respectively. Their survival rate after simulated gastrointestinal test were 12.42 ± 2.84%, 9.10 ± 1.12%, 5.86 ± 0.52%, and 8.82 ± 2.50% and their adhesion rates to Caco-2 cell were 6.09 ± 0.39%, 6.37 ± 0.28%, 6.94 ± 0.27%, and 6.91 ± 0.11%, respectively. The orthogonal tests of bacterial powders of the four strains showed that the maximum inhibitory activities to α-amylase and α-glucosidase were 93.18 ± 1.19% and 75.33 ± 2.89%, respectively. The results showed that the mixture of Lactobacillaceae could lower FBG, reduce inflammation, and liver, kidney, and pancreas damage, promote PI3K/AKT signaling pathway, and increase the content of SCFAs. The combination of L. plantarum 22, L. plantarum 25, L. fermentum 11, and L. fermentum 305 can potentially improve type 2 diabetes mellitus (T2DM).
Kestose Increases the Relative Abundance of Faecalibacterium spp. and Nominally Increases Cow Milk Tolerant Dose in Children with Cow's Milk Allergy – Preliminary Results
Authors: Kubota Shohei , Sugiura Shiro , Takahashi Mayuko , Kadota Yoshihiro , Takasato Yoshihiro , Matsui Teruaki , Kitamura Katsumasa , Tochio Takumi , Ito Komei 
Published: September 2023

Abstract
A single-arm study was conducted with 10 children aged 2–12 years with severe cow's milk allergy (CMA) requiring complete allergen elimination. Subjects were administered kestose, a prebiotic, at 1 or 2 g/day for 12 weeks. Results of a subsequent oral food challenge (OFC) showed a statistically significant increase in the total dose of cow's milk ingestion (1.6 ml vs. 2.7 ml, p = 0.041). However, the overall evaluation of the OFC results, TS/Pro (total score of Anaphylaxis Scoring Aichi (ASCA)/cumulative dose of protein), showed no statistically significant improvement, although the values were nominally improved in seven out of 10 subjects. The 16S rDNA analysis of fecal samples collected from the subjects revealed a statistically significant increase in the proportion of Faecalibacterium spp. (3.8 % vs. 6.8%, p = 0.013), a type of intestinal bacterium that has been reported to be associated with food allergy. However, no statistically significant correlation was found between Faecalibacterium spp. abundance and the results of the OFC.
The Prokaryotic Microalga Limnothrix redekei KNUA012 to Improve Aldehyde Decarbonylase Expression for Use as a Biological Resource
Authors: Kim Young-Saeng , Baek Haeri , Yun Hyun-Sik , Lee Jae-Hak , Lee Kyoung-In , Kim Han-Soon , Yoon Ho-Sung 
Published: September 2023

Abstract
The prokaryotic microalga Limnothrix redekei KNUA012 isolated from a freshwater bloom sample from Lake Hapcheon, Hapcheon-gun, South Korea, was investigated for its potential as a biofuel feedstock. Microalgae produce straight-chain alkanes/alkenes from acyl carrier protein-linked fatty acyls via aldehyde decarbonylase (AD; EC 1.2.1.3), which can convert aldehyde intermediates into various biofuel precursors, such as alkanes and free fatty acids. In L. redekei KNUA012, long-chain ADs can convert fatty aldehyde intermediates into alkanes. After heterologous AD expression in Escherichia coli (pET28-AD), we identified an AD in L. redekei KNUA012 that can synthesize various alkanes, such as pentadecane (C15H32), 8-heptadecene (C17H34), and heptadecane (C17H36). These alkanes can be directly used as fuels without transesterification. Biodiesel constituents including dodecanoic acid (C13H26O2), tetradecanoic acid (C15H30O2), 9-hexa decenoic acid (C17H32O2), palmitoleic acid (C17H32O2), hexadecanoic acid (C17H34O2), 9-octadecenoic acid (C19H36O2), and octadecanoic acid (C19H38O2) are produced by L. redekei KNUA012 as the major fatty acids. Our findings suggest that Korean domestic L. redekei KNUA012 is a promising resource for microalgae-based biofuels and biofuel feedstock.
Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot
Authors: Li Si , Zhang Fu-Mei , Shang Xiao-Jing , Hou Rui 
Published: September 2023

Abstract

Fusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on F. oxysporum and F. commune. We found that the inhibitory effects of TM11 volatiles and broth metabolites on F. oxysporum were significant, but only F. commune volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of F. oxysporum and F. commune. The pot experiment showed that TM11 had significant control effects against F. oxysporum and F. commune, and inoculation of TM11 prior to that of F. oxysporum and F. commune was more effective. The TM11, TM11 and F. oxysporum, or F. commune and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and F. oxysporum or F. commune > distilled water. The results showed that TM11 provided effective control of blueberry root rot.
Aspek Teknis Budidaya Dan Profitabilitas Pendederan Ikan Nila Merah (Oreochromis niloticus) Studi Kasus Di Turbo Farm, Kota Bogor Jawa Barat
Authors: Vinasyiam Apriana , Hadiroseyani Yani , Agustiyana Chandrika 
Published: September 2023

Nursery culture of tilapia (Oreochromis niloticus) is an important segment to support a comprehensive tilapia culture business. Requirements for business sustainability involve the high profitability of the company and high growth of the reared fish. This experiment aimed to observe the profitability aspect, fish growth, water quality, and culture techniques applied in a tilapia farm. The experiment was done using case study method, where Turbo farm, located in Bogor West Java, was used as a model. The data collected involved primary data from field observation and secondary data from interview activities. The results showed that tilapia juvenile had a good growth, and that the water quality (temperature and pH) ranged within optimal range for tilapia culture. At stocing density of 2.000 juvenile per production unit in one cycle, the total production was 68.400 juvenile per year, in which 10% of the total produced juvenile were sold at size of 7-8 cm, while the remaining juveniles were at size 11-12 cm. Price of juvenile at size 7-8 cm was half of the size 11-12 cm, Rp 600 vs Rp 1.200 per juvenile, respectively. The profit of the business was Rp 43.989.000 per year with value of revenue/cost (R/C) ratio 2,29, and payback period (PP) 6,27 year. Based on the previously mentioned profitability parameters, the nursery culture of tilapia performed by Turbo farm was profitable. However, an efficiency of facility use was needed to increase profit and shorten the return on investment period.
Faktor-Faktor Risiko yang Berhubungan dengan Hipertensi di Kelurahan Belawan 1
Authors: Rezha Diandra Kayladifa , Hasibuan Rapotan , Maipiana Data Rizqueen , Lubis Citra Mutiara , Difhanny Charunnisyah Nurma , Marpaung Suci Yustrianti 
Published: September 2023

Hypertension is also called the “silent killer” because people with hypertension often have no symptoms. The National Institute of Heart, Lung and Blood in Indonesia estimates that half of people who suffer from hypertension are not aware of their condition. The purpose of this study was to identify the factors associated with the onset of hypertension in the Belawan 1 Village area. This study used an analytical study with a case control approach. The sample of this study was 236 respondents divided by a ratio of 1:1, namely 118 people in the control group and 118 people in the case group. The results of factor analysis of excessive salt consumption, lack of physical activity, family history of disease, consumption of excess fat, lack of fruits and vegetables, and excessive sugar consumption obtained pValue = 0.000, 0.016, 0.020, 0.040 0.042, 0.049 which means there is a relationship between physical activity factors, smoking habits, obesity and stress with the onset of hypertension. Then the results of the risk factor analysis were retested using logistic regression to see which factors were the most influential among the independent variables with the incidence of hypertension, and the results obtained were excessive salt consumption, lack of physical activity, family history of disease, and lack of fruits and vegetables with AOR. = 0.216, 0.389, 0.339, 0.459. For this reason, it is recommended for health workers to provide counseling about hypertension, the causative factors and how to live a healthy and regular lifestyle so as to avoid hypertension.
Pengaruh Digital Marketing terhadap Peningkatan Volume Penjualan Kamar di The Patra Bali Resort & Villas
Authors: Sari P Luh Gede Putri , Suhardi Suhardi 
Published: September 2023

This study aims to find out and analyze the influence of digital marketing consisting of Websites, Social Media and Online Travel Agents (OTA), on the volume of sales increase in the recovery period at The Patra Bali Resort & Villas. This research uses quantitative data types and takes the form of associative research. The population in this study is all consumers of The Patra Bali Resort & Villas. The samples in this study are included in the category of non-probability sampling with the purposive sampling method. The sample criteria in this study were consumers (tourists and/or visitors) of The Patra Bali Resort & Villas who made transactions (bookings) through digital marketing channels, namely: email, social media (IG) and OTA). The method of data collection is carried out by distributing questionnaires. The data analysis method uses multiple linear regression analysis. The results of the analysis in this study show that digital marketing via email has a positive effect on the volume of sales increase at The Patra Bali Resort & Villas. This means that the higher and more effective the implementation of digital marketing via email, the more it will affect the increasing volume of sales at The Patra Bali Resort & Villas. Digital marketing through social media (Instagram) positively affects the volume of sales increase at The Patra Bali Resort & Villas. This means that the more effective the implementation of digital marketing via email, the more it will increase the volume of sales increase. Digital marketing through Online Travel Agents positively affects the volume of sales increase at The Patra Bali Resort & Villas. This means that the higher and more effective the application of Online Travel Agent, the more it will affect the increasing volume of sales at The Patra Bali Resort & Villas.
Optimalisasi Pemeliharaan Jalan Wilayah I Dinas Binamarga, Cipta Karya dan Tata Ruang Provinsi Sumatera Barat
Authors: Rita Eva , Carlo Nasfryzal , Anwar Khairul 
Published: September 2023

The occurrence of road damage causes road conditions to be less stable. This was triggered by the ineffective implementation of road maintenance in Region I Dinas Binamarga, Cipta Karya, and Tata Ruang provinsi Sumatra Barat. For this reason, efforts and strategies are needed so that road maintenance work becomes optimal and the road can reach the specified design age so that the road in question can be used safely, comfortably, and safely. To obtain the intended solution, a study was carried out on what factors influenced the effectiveness of road maintenance in area I of the Highways, Human Settlement, and Spatial Planning Offices of West Sumatra Province. This study uses descriptive qualitative and evaluative methods through literature study and primary data collection by interviewing predetermined informants. Halil's study shows that there are nine factors for the effectiveness of road maintenance, namely responsiveness, responsibility, community support, funds/budgets, time, human resources, coordination between policymakers, organizational effectiveness, and material resources. There are three dominant factors influencing the effectiveness of road maintenance, namely funds/budget, time, and human resources. SWOT analysis is used to determine the optimal solution to obtain the weight of the IE diagram (x, y = -0.85, -0.425) so that the proposed strategy is to maintain and maintain the existing system by carrying out periodic road damage surveys to find out damage so that potential damage and how to handle it, calculating budget requirements, determining priority scales for road maintenance, determining patterns of work implementation either by self-management or in collaboration with third parties, or collaboration with local communities, placing competent and experienced personnel and adjusting the number of road observers with the length of the existing roads.