bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Compact and Hermetic Organic Rankine Cycle Turbine Generator With Upgraded Cooling Capability
Authors: Tanaka Toshimitsu , Takata Ryo , Hirado Takuma , Kawanami Akira 
Published: January 2025

Abstract
In this article, a hermetic and oil-free turbine generator for a cryogenic organic Rankine circle (ORC) is suggested and investigated. The generator mainly consists of a high-speed generator, a two-stage supersonic turbine, and magnetic bearings. Based on the hermetic design concept, these components are in a common casing. To upgrade the cooling capability of the generator, heat dissipation fins are installed between the first and second stages of the turbine. Performance of the turbine generator was preliminarily investigated by CFD calculations. These results show that the turbine efficiency peaks at 16,000 rpm under the design pressure ratio condition. Under the same condition, it is also predicted that comparatively high static pressure recovery coefficient and low total pressure loss can be obtained through the flow path with the fins. Moreover, a series of experimental campaign were conducted on an ORC test rig using −196 °C liquid nitrogen and 50 °C warm water as cold source and heat source, respectively. These results indicate that the target power output was achieved and the installed heat dissipation fins are effective to enhance the cooling capability of the generator. It was also indicated that under comparatively high loaded conditions, the turbine power output can be increased by heat transferred from the generator.
Comparing two-sample log-linear exposure estimation with Bayesian model-informed precision dosing of tobramycin in adult patients with cystic fibrosis
Authors: Tong Dominic M. H. , Hughes Maria-Stephanie A. , Hu Jasmine , Pearson Jeffrey C. , Kubiak David W. , Dionne Brandon W. , Hughes Jasmine H. 
Published: January 2025

ABSTRACT

Tobramycin dosing in patients with cystic fibrosis (CF) is challenged by its high pharmacokinetic (PK) variability and narrow therapeutic window. Doses are typically individualized using two-sample log-linear regression (LLR) to quantify the area under the concentration-time curve (AUC). Bayesian model-informed precision dosing (MIPD) may allow dose individualization with fewer samples; however, the relative performance of these methods is unknown. This single-center retrospective analysis included adult patients with CF receiving tobramycin from 2015 to 2022. Tobramycin concentrations were predicted using LLR or Bayesian estimation with two population PK models (Hennig and Alghanem). Then, both methods were used to estimate the AUC for simulated patients. For Bayesian estimation, AUC estimation with flattened priors and limited sampling strategies were also assessed. Predictions were evaluated using normalized root mean square error (nRMSE), mean percent error (MPE), and accuracy. The data set included 70 treatment courses, with 32 not evaluable by LLR due to detection limits or timing issues. Bayesian estimation demonstrated worse accuracy (47.1%–50.7% vs 75.7%), higher MPE (24.2%–32.4% vs −2.4%), and higher nRMSE (35.0%–39.4% vs 24.8%) than LLR for peak concentrations but performed better on troughs (accuracy: 92.0%–92.9% vs 84.6%). Bayesian estimation with flattened priors and a single sample at 4 h was comparable to LLR performance, with better accuracy (42.9%–68.0% vs 41.1% LLR), comparable MPE (−2.3% to −3.7% vs −0.5%) and nRMSE (11.3%–21.6% vs 17.3%). Bayesian estimation with one concentration and flattened priors can match LLR prediction accuracy. However, popPK models must be improved to better estimate peak samples.
Advancements in the fight against globally distributed OXA-48 carbapenemase: evaluating the new generation of carbapenemase inhibitors
Authors: Outeda-García Michelle , Arca-Suárez Jorge , Lence Emilio , Rodriguez-Coello Arianna , Maceiras Romina , Blanco-Martin Tania , Guijarro-Sánchez Paula , Gonzalez-Pinto Lucia , Alonso-Garcia Isaac , García-Pose Andrea , Muras Andrea , Rodriguez-Pallares Salud , Lasarte-Monterrubio Cristina , Gonzalez-Bello Concepción , Vázquez-Ucha Juan Carlos , Bou German , Beceiro Alejandro 
Published: January 2025

ABSTRACT

Carbapenemase OXA-48 and its variants pose a serious threat to the development of effective treatments for bacterial infections. OXA-48-producing Enterobacterales are the most prevalent carbapenemase-producing bacteria in large parts of the world. Although these bacteria exhibit low-level carbapenem resistance
in vitro
, the infections they cause are challenging to treat with conventional therapies, owing to their spread and complex detection in clinical settings. However, numerous β-lactamase inhibitors (BLIs) are currently in the pipeline or late clinical stages. To assess the potential of these compounds, this study compared the efficacy against OXA-48 of novel β-lactamase inhibitors, specifically the 1,6-diazabicyclo[3,2,1]octanes (DBOs) avibactam, relebactam, zidebactam, nacubactam, and durlobactam, along with the cyclic and bicyclic boronates vaborbactam, taniborbactam, and xeruborbactam. The extensive kinetics assays identified xeruborbactam, taniborbactam, and durlobactam, together with the already established avibactam, as BLIs with superior biochemical performance. Susceptibility testing further validated these findings but also demonstrated significantly improved bacterial killing by the DBOs zidebactam, nacubactam, and durlobactam. On the other hand, binding studies demonstrated the superior inhibitory capacity of the BLIs durlobactam and xeruborbactam. Combinations, such as cefepime/zidebactam, meropenem/nacubactam, and sulbactam/durlobactam, show promising activity against OXA-48-producing Enterobacterales, while ceftazidime/avibactam, cefepime/taniborbactam, and meropenem/xeruborbactam combinations also appear highly active, largely due to the excellent kinetics of these new inhibitors. Overall, this comprehensive analysis provides important insights into the effectiveness of new BLIs against OXA-48-producing Enterobacterales, highlighting xeruborbactam, durlobactam, and avibactam as leading candidates. Additionally, BLIs like zidebactam, nacubactam, and taniborbactam also showed potential in addressing the clinical challenges posed by OXA-48-mediated antimicrobial resistance.
Cultivation and mating of the truffle
Tuber japonicum
in plantations of ectomycorrhizal
Quercus serrata
seedlings
Authors: Nakamura Noritaka , Kinoshita Akihiko , Nakano Shota , Furusawa Hitomi , Obase Keisuke , Yamaguchi Muneyoshi , Noguchi Kyotaro , Kitade Yuki , Yamanaka Takashi 
Published: January 2025

ABSTRACT

Tuber japonicum
, a white-colored truffle that is endemic to Japan, is promising for culinary purposes due to its unique aroma. We were able to cultivate
T. japonicum
in plantations of inoculated
Quercus serrata
seedlings for the first time. Ascocarps were found after 43 months at one site and after 61 months at another. We developed simple sequence repeat markers for multilocus genotyping of glebal tissue and ascospores and confirmed that the harvested ascocarps were derived from inocula. All paternal individuals matched the multilocus genotypes of neighboring maternal individuals, indicating frequent hermaphroditism and the absence of externally introduced individuals. Our findings provide important clues to understanding the reproductive biology of
T. japonicum
during the early period after the truffle plantation establishment.

IMPORTANCE

Truffles are highly prized as a delicacy, but only a select few species have been successfully cultivated. In our study, we succeeded for the first time in cultivating
Tuber japonicum
. Two out of four plantations produced ascocarps shortly after planting, with one of them yielding a comparable weight to other cultivated truffle species. This promising productivity suggests that the fungus has potential when cultivated. Our analysis of the ascocarps' maternal and paternal genotypes, using simple sequence repeat markers, revealed hermaphroditic behavior in the fungus at our planting site. Our findings provide crucial insights into the truffle mating events.
Ligilactobacillus salivarius
LZZAY01 accelerated autophagy and apoptosis in colon cancer cells and improved gut microbiota in CAC mice
Authors: Yang Wenhong , Li Tao , An Shixiang , Chen Rong , Zhao Yuxin , Cui Jiaxian , Zhang Mingyu , Lu Jingkun , Tian Yunpeng , Bao Lili , Zhao Pengwei 
Published: January 2025

ABSTRACT

Colorectal cancer (CRC) is one of the malignant tumors globally, with high morbidity and mortality rates. The mainstay treatment of CRC includes surgery, radiotherapy, and chemotherapy. However, these treatments are associated with a high recurrence rate, poor prognosis, and highly toxic side effects. The probiotics have the potential to prevent CRC, and they display a favorable safety performance. Probiotics could provide a potential strategy to prevent and treat CRC. The impact of LZZAY01 on cancer cell lines CT-26, HCT-116, and SW-620 was evaluated by conducting cytotoxicity and clonogenicity tests. A model of colitis-associated cancer (CAC) was established in C57BL/6j mice following induction with AOM/DSS. The levels of autophagy and apoptosis proteins, tight junction proteins, and inflammatory factors were detected by western blotting, immunofluorescence assay, and enzyme-linked immunosorbent assay. High-throughput sequencing of gut 16S rRNA was performed to analyze the abundance and diversity of the gut microbiome. LZZAY01, a new strain of
Ligilactobacillus salivarius
, was certified by an evolutionary tree and average nucleotide identity. LZZAY01 enhanced autophagy and apoptosis in CT-26, HCT-116, and SW-620 cell lines. It preserved the integrity of the intestinal barrier by regulating the tight junction protein ZO-1 and claudin-1. The tumor necrosis factor-α and interleukin-6 were reduced by LZZAY01. The abundance and diversity of the intestinal microbiota were enhanced, especially the beneficial bacterial species maintaining the balance of the intestinal flora such as
Bifidobacterium
and
Lactobacillus
.
L. salivarius
LZZAY01 improved CAC
via
suppressing the growth of colon cancer cells, promoting autophagy and apoptosis, enhancing intestinal tight junctions, reducing intestinal barrier degradation, modifying the gut microbiota abundance, and decreasing inflammatory reactions.

IMPORTANCE

Although similar probiotics have been shown to have anticancer potential in colorectal cancer (CRC), there is a paucity of research related to the preventive function of probiotics against CRC. And there are fewer studies about the mechanism of probiotics’ preventive effects on CRC. The regulation of tumor cell proliferation and apoptosis by the active ingredients of probiotics may be one of the mechanisms of their prevention of CRC. In this study, we explored the effects of
L. salivarius
LZZAY01 on autophagy and apoptosis of colon cancer cells
in vitro
and
in vivo
and proposed a possible mechanism for the prevention of CRC by probiotics.
Genomic characterization of noroviruses from an outbreak associated with oysters
Authors: Flint Annika , Harlow Jennifer , McLeod Madison , Blondin-Brosseau Madeleine , Weedmark Kelly , Nasheri Neda 
Published: January 2025

ABSTRACT

Human noroviruses are the leading cause of non-bacterial shellfish-associated gastroenteritis. In 2022, a multi-jurisdictional norovirus outbreak associated with contaminated oysters occurred that involved hundreds of illnesses. Here, we conducted genetic analysis on 30 clinical samples associated with this oyster outbreak. We first determined the capsid genotypes by Sanger sequencing and viral titers by droplet-digital reverse transcription PCR. Multiple genotypes were identified in this outbreak, which could indicate contamination with wastewaters. The majority of samples belonged to GII.3[P12], followed by GII.2[P16], GII.17[P17], and GII.4 Sydney[P16]. We next proceeded with whole-genome sequencing and obtained full genomes for 19 samples. Phylogenetic analysis revealed that some of the isolates showed high similarity with the sequences isolated from the United States related to the same outbreak. We also analyzed amino acid variations in the sequenced genomes and found that overall the GII.3[P12] isolates have lower variations compared to other genotypes.

IMPORTANCE
Norovirus outbreaks associated with contaminated shellfish occur frequently. Whole-genome sequencing (WGS) could play a critical role in understanding and controlling norovirus outbreaks as it allows for source attribution, tracking transmission pathways, and detecting recurrent or linked outbreaks. Here, we described how the data obtained by WGS were employed for understanding transmission patterns and norovirus epidemiology.
Maternal perinatal anxiety and infant primary care use in 1998–2016: a UK cohort study
Authors: Smith Holly Christina , Archer Charlotte , Bailey James , Chew Graham Carolyn , Evans Jonathan , Fisher Tamsin , Kessler David , Kingstone Tom , Procter Janine , Shivji Noureen , Silverwood Victoria , Spruce Amy , Turner Katrina , Wu Pensée , Yu Dahai , Petersen Irene 
Published: January 2025

BackgroundThere is some evidence that perinatal anxiety (PNA) is associated with lower rates of infant vaccinations and decreased access to preventative infant healthcare, but results across studies have not been conclusive.ObjectiveTo investigate the relationship between maternal PNA and infant primary care use.MethodsCohort study of mother-infant pairs identified between 1998 and 2016 using IQVIA Medical Research Database (IMRD). PNA was identified through prescription, diagnosis and symptom records from start of pregnancy up to 1 year after birth. Outcomes include primary care consultation rate, attendance at the 6–8 week infant check and uptake of the infant 5-in-1 vaccination, comparing unadjusted rates of consultations and using logistic regression to analyse other outcomes.FindingsOf the 248 618 women, 11 558 (4.7%) had a record of PNA. Infants of mothers with PNA had, on average, one more primary care consultation/person-year compared with those without (9.7 vs 8.7) in the year after birth. Mothers with PNA were more likely to have an infant who was vaccinated (adjusted OR (aOR) 1.33, 95% CI 1.20 to 1.48) but were less likely to have a record of attendance at the 6–8 week infant check (aOR 0.88, 95% CI 0.81 to 0.95).ConclusionsInfants of mothers with PNA had, on average, a slightly higher rate of primary care consultations and were more likely to be vaccinated but less likely to have a record of an infant check.Clinical implicationsMidwives and General Practitioners (GPs) providing care should consider how PNA may impact on infant health and how infant health may impact on maternal anxiety.
Sex hormone-dependent host-microbiome interactions and cardiovascular risk (XCVD): design of a longitudinal multi-omics cohort study
Authors: Franz Kristina , Markó Lajos , Mähler Anja , Chakaroun Rima , Heinitz Sascha , Schlögl Haiko , Sacher Julia , Steckhan Nico , Dechend Ralf , Adams Noah , Andersen Marianne , Glintborg Dorte , Viehweger Martin , Bahr Lina Samira , Forslund-Startceva Sofia Kirke 
Published: January 2025

IntroductionCardiovascular diseases (CVDs) present differently in women and men, influenced by host-microbiome interactions. The roles of sex hormones in CVD outcomes and gut microbiome in modifying these effects are poorly understood. The XCVD study examines gut microbiome mediation of sex hormone effects on CVD risk markers by observing transgender participants undergoing gender-affirming hormone therapy (GAHT), with findings expected to extrapolate to cisgender populations.Methods and analysesThis observational, longitudinal cohort study includes baseline, 1- and 2-year follow-ups with transgender participants beginning GAHT. It involves comprehensive phenotyping and microbiome genotyping, integrating computational analyses of high-dimensional data. Microbial diversity will be assessed using gut, skin, and oral samples via 16S rRNA and shotgun metagenomic sequencing of gut samples. Blood measurements will include sex hormones, CVD risk markers, cardiometabolic parameters, cytokines, and immune cell counts. Hair samples will be analysed for cortisol. Participants will complete online questionnaires on physical activity, mental health, stress, quality of life, fatigue, sleep, pain, and gender dysphoria, tracking medication use and diet to control for confounders. Statistical analyses will integrate phenomic, lifestyle, and multi-omic data to model health effects, testing gut microbiome mediation of CVD risk as the endocrine environment shifts between that typical for cisgender men to women and vice versa.Ethics and disseminationThe study adheres to Good Clinical Practice and the Declaration of Helsinki. The protocol was approved by the Charité Ethical Committee (EA1/339/21). Signed informed consent will be obtained. Results will be published in peer-reviewed journals and conferences and shared as accessible summaries for participants, community groups, and the public, with participants able to view their data securely after public and patient involvement review for accessibility.Trial registration numberThe XCVD study was registered on ClinicalTrials.gov (NCT05334888) as ‘Sex-differential host-microbiome CVD risk — a longitudinal cohort approach (XCVD)" on 4 April 2022. Data set link can be found athttps://classic.clinicaltrials.gov/ct2/show/NCT05334888.
Evaluation of the implementation in primary care of genetic testing for the screening of MODY2 (iMOgene): protocol for an implementation pilot study
Authors: Legeay Samuel , Poitras Marie-Eve , Tremblay-Vaillancourt Vanessa , Fournier Amélie , Bouchard Luigi , Leblanc Josianne , Caron Philippe , Savard Catherine , Bettez Nathalie , Tremblay Karine 
Published: January 2025

IntroductionMODY2 (maturity-onset diabetes of the young type 2, MIM125851) is a monogenic diabetes with an autosomal dominant transmission caused by a variant of theGCKgene. MODY2 is often confused with type 1 or type 2 diabetes, but despite a slightly elevated blood glucose level, it does not induce long-term vascular complications, nor does it require pharmacological treatment. Genetic testing for the diagnosis of MODY2 is currently reserved for genetic specialists and some physicians. Still, access to it by primary care healthcare professionals (HCPs), coupled with appropriate training, would improve the diagnosis and management of patients with MODY2. Thus, to evaluate the implementation in primary care of genetic testing for the screening of MODY2 (iMOgene study), an implementation pilot study has been designed supported by the RE-AIM (Reach, Effectiveness, Adoption, Implementation, Maintenance) framework.Method and analysisTwo primary care clinics will be involved in the region of Saguenay-Lac-Saint-Jean (Québec, Canada). An asynchronous online training on MODY2 and genetic testing, including pre/post questionnaires, will be provided to the HCPs. Satisfaction, adoption and maintenance indicators will be collected throughout the project for each clinic. Questionnaires for patients and professionals and focus groups with HCPs will be conducted to assess implementation. This study will document the implementation process of genetic testing in primary care by identifying facilitating and limiting factors to establish specifications for scaling up.Ethics and disseminationThe present protocol has been approved by the research ethic committee of the ‘Centre intégré universitaire de santé et de services sociaux of Saguenay-Lac-Saint-Jean’(CIUSSS-SLSJ) on 9 January 2024 and by the ‘Comité central d’éthique de la recherche’ (CCER) of the ‘Ministère de la Santé et des Services Sociaux’ of Quebec (Canada) on 30 January 2024. The informed consent of participants will be obtained orally. Dissemination of the study results will involve peer-review publications, presentations at major national and international scientific conferences.
Mobile health applications for older people in Asia: protocol for a systematic review of end-user perceptions and recommendations
Authors: Leung Wilson , Lam Simon Ching , Sum Kim Wai Raymond , Yang Yijian , Chan Ching Lam , Chow Janice Ngar Lam , Wong Yvonne , Cheung Jasmine , Shum Edward , Yip Agnes Wing Ki , Wan Winsy Wing Yu , Luk Kevin , Ha Kong Nam , Suen Lorna Kwai Ping 
Published: January 2025

IntroductionMobile technology has revolutionised the way people interact with others and gain access to healthcare services. Given that cultural background is a strong moderator for technology penetration, this systematic review aims to examine end-user perceptions and design recommendations for mobile health applications among Asian older people.Methods and analysisFive electronic databases (PubMed, CINAHL, PsycINFO, Medline and Cochrane Central Register of Controlled Trials) will be searched until May 2025. Studies conducted on Asian older people aged 60+ years, with English/Chinese full text available, will be included. Narrative approaches and effect direction plots will be used for data analyses. Risk of bias across studies will be examined using Cochrane Risk of Bias 2 and Risk of Bias in Nonrandomised Studies of Interventions, whereas the quality of evidence will be assessed by Shekelle’s classification scheme.Ethics and disseminationNo ethical approval will be required. The findings will be disseminated through peer-reviewed journal articles.PROSPERO registration numberCRD42024562861.
Content and outcome of non-pharmacological rehabilitation in hospital, or community-based care, for women with traumatic brain injury: a scoping review protocol
Authors: Holmqvist Kajsa Lidström , Strandberg Thomas , Simpson Grahame , Massey Jessica , Matérne Marie 
Published: January 2025

IntroductionTraumatic brain injury (TBI) is a global health issue and a leading cause of long-term disabilities and mortality worldwide. There is growing evidence that TBI rehabilitation should be differentiated and individualised according to gender to provide more effective healthcare and rehabilitation. However, there is a lack of reviews focusing on the rehabilitation for women with TBI and there is a need to summarise existing knowledge to guide and individualise their rehabilitation. This scoping review aims to identify and map evidence on content and outcome of non-pharmacological rehabilitation for women with TBI aged below 65 years.Methods and analysisThis scoping review will follow the methodological guidelines of the Joanna Briggs Institute (JBI) and reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for scoping reviews (PRISMA-ScR). The databases searched will be PubMed, CINAHL and PsycINFO. The following inclusion criteria will be applied: peer-reviewed studies published in English over the years 2000–2024 including description of content and outcomes of non-pharmacological TBI rehabilitation for women aged between 16 and 65 years in both inpatient and outpatient contexts. All severities of TBI ranging from concussion through to extremely severe will be included. Text and opinion papers, conference abstracts and grey literature will be excluded. Studies fulfilling the inclusion criteria will be independently reviewed by three researchers. A data extraction form will be used including specific details about the participants, concept, context, study methods and key findings. The results will be presented in tabular format accompanied by a narrative summary.Ethics and disseminationDue to the nature of data, no approval from an ethics committee is required. Dissemination of results are planned in an open-access peer-reviewed journal and in professional networks.Review registration numberOSF,https://doi.org/10.17605/OSF.IO/QUY3T.