bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bufalin targets the SRC-3/c-Myc pathway in chemoresistant cells to regulate metastasis induced by chemoresistance in colorectal cancer
Authors: Chen Jinbao , Wu Chenqi , Yu Kun , Tang Xiaoxia , Xu Ke , Chen Yi , Li Wei 
Published: February 2023

Abstract
Metastasis and chemoresistance are often major challenges in advanced-stage colorectal cancer. Recent studies have found extensive crosstalk between them. Previous studies have shown that bufalin has a therapeutic effect on both metastasis and drug resistance, but how bufalin affects chemoresistance-mediated metastasis remains unclear. In our study, we found that bufalin inhibited resistance-induced epithelial-mesenchymal transition (EMT) and angiogenesis, which in turn inhibited the resulting metastasis. In addition, we demonstrated that targeting of the SRC-3 protein by bufalin reduced the expression level of c-Myc and inhibited the prometastatic effect mediated by chemoresistance. Overexpression of SRC-3 or c-Myc reversed the inhibitory effect of bufalin on chemotherapeutic resistance, promoting metastasis. More interestingly, we also found that the clinical drug cinobufacini and its main active monomer bufalin reduced liver metastasis of colorectal cancer caused by chemoresistance in vivo. In conclusion, bufalin can target the SRC-3/c-Myc signaling pathway to affect the prometastatic effect of chemoresistant cells, suggesting that bufalin may be used as a new adjuvant antimetastatic therapy for colorectal cancer.
Association between gastrointestinal perforation and patent ductus arteriosus in extremely-low-birth-weight infants: A retrospective study of our decade- long experience
Authors: Muto Mitsuru , Sugita Koshiro , Murakami Masakazu , Ikoma Shinichiro , Kawano Masato , Masuya Ryuta , Matsukubo Makoto , Kawano Takafumi , Machigashira Seiro , Nakame Kazuhiko , Torikai Motofumi , Ikee Takamasa , Noguchi Hiroyuki , Ibara Satoshi , Ieiri Satoshi 
Published: February 2023

Abstract
Purpose
Management of persistently patent ductus arteriosus (PDA) in extremely-low-birth-weight infants (ELBWIs) requires attention due to the risk of tissue hypoperfusion. We investigated the association between PDA and gastrointestinal perforation.
Methods
We performed a retrospective chart review from 2012 to 2021. Preterm (≤ 32 weeks) ELBWIs with PDA after birth who developed necrotizing enterocolitis (NEC), focal intestinal perforation (FIP), and idiopathic gastric perforation were included; ELBWIs with congenital heart disease were excluded. Data were analyzed using chi-squared tests with Yates' correction, and Student's t-test.
Results
Five hundred thirty-five preterm ELBWIs were analyzed, including 20 with NEC, 22 with FIP, and 1 with gastric perforation. In NEC and FIP, the ductus arteriosus remained open in 40% (4/10) and 63.6% (14/22) of cases, respectively, and cyclo-oxygenase inhibitor treatment showed poor efficacy (p = 0.492 and 0.240). The incidence of perforation in NEC (4/9 vs. 6/11, p = 0.653), mortality in NEC (3/4 vs. 3/6, p = 0.895) and FIP (6/14 vs. 3/8, p = 0.838) did not differ according to whether the PDA persisted or resolved.
Conclusion
The presentation of PDA did not affect mortality or morbidity of ELBWIs. However, it is essential to consider the possibility of gastrointestinal perforation due to decreased organ blood flow caused by ductal steal.
Exploring Exploration – How to Look for Deep- Sea Minerals
Authors: Trellevik Lars-Kristian 
Published: February 2023

Abstract
In the spring of 2023, the subsea industry in Norway is gearing up for an opening of the Norwegian continental shelf (NCS) for deep-sea mineral exploration and extraction. Existing research indicates that the profitability of deep-sea mining with today's technology heavily depends on high ore grades. This study explores the potential for emerging techno-operational concepts for exploration to affect the profitability of Seafloor Massive Sulfides (SMS) Industry on the NCS. By way of computer simulation, the study analyzes technologies currently, or soon, poised to enter the subsea market, with regard to their advantage or disadvantage for the SMS industry on the NCS. The study indicates that there is significant advantage in developing geophysical sampling technology for assessing mineral resources and some advantage in developing unmanned surface vessels for regional surveys. It further indicates that developing fleet operated autonomous underwater vehicle concepts for high-resolution surveys is not only moot, but possibly counterproductive. The study thereby contributes techno-operational insight for a budding industry currently looking for technology to improve commercial prospects.
Modeling of Water-invaded Horizontal Well With Inflow Control Device's at Gulf of Suez Area
Authors: Mohamed abdalla , Al-Fakharany Taher , Khalid Samir 
Published: February 2023

Abstract
The Gulf of Suez contains a lot of fields most of these fields suffering from water invasion. Inflow control technology is used to manacle the unfavorable effects of water coning that delay water breakthrough. It balances the inflow between well bore and completion by adding more pressure drop between the reservoir and the well bore. This paper describes the first models of using inflow control devices in Gulf of Suez wells and such technique in Egypt and also compare this modeling of prediction data with the actual data for horizontal well already completed and produced from long time suffered from water infringement. The analysis was done for heterogeneity reservoir with different models using ICD and AICV that assumed three different cases and compared to actual open hole well. The results showed that the well completed with ICD and AICV significantly higher oil production performance, meanwhile ICD is somewhat getting better than autonomous ICV. Inflow control device technique would be able to delay water production for long time than the actual perforated open hole well.
Cobalt nanostructures electrochemical nucleation from a type-IV deep eutectic solvent based on CoCl 2 ·6H 2 O and ethylene glycol
Authors: Hoang Thi Thanh Thuy , Ho Nang Xuan , Duy Vinh Nguyen , Thi Thuy Cao , Anh Tuan Pham , Morales-Gil Perla , Palomar-Pardave Manuel , Hieu Nguyen , Chu Thi-Xuan , Manh Tu Le 
Published: February 2023

Abstract

In the present work, electrodeposition and electronucleation of cobalt on a glassy carbon electrode from a type-IV deep eutectic solvent, mixture of CoCl2·6H2O and ethylene glycol, were studied. It was found, by cyclic voltammetry, that cathodic efficiencies of Co electrodeposition were high for both cases without and with supporting electrolyte, yielding their values of 95 % and 96 %, respectively, which helped to consider negligible contribution of side reaction during the electrodeposition process. The selection of used supporting electrolyte concentration was based on Gibbs free energy for nucleation criterion. The presence of supporting electrolyte improved the voltammogram’s shape and reduction current density by controlling the system under diffusional regime. From chronoamperometry, it was established that the instantaneous nucleation was confirmed to be predominant at lower deposition potentials (i.e., E < -0.90 V), while progressive nucleation was governed at higher potentials (i.e., E > -0.92 V) during the Co electrodeposition. Current density transients of the Co(II) system could be analyzed by the three-dimensional nucleation and diffusion-controlled growth model of Co metallic nuclei, to derive kinetic parameters such as frequency of nucleation, A, number of active sites, N0, and diffusion coefficient, D, of the Co (II) in type-IV DES system. Moreover, excellent quality of Co electrodeposits with different morphological features was obtained from the type-IV eutectic mixture and it was even better without the use of the supporting electrolyte, for the same applied potential and electrodeposition time. Surface characterization methods, including scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Raman spectroscopy, have revealed the presence of high purity electrodeposited Co nanoparticles on the electrode surfaces. Both cases, with and without supporting electrolyte, produced cobalt electrodeposited nanostructures with the hexagon-close-packed crystal lattice, which was verified by X-ray measurements.
Hydrogen sulfide promotes the stemness of liver cancer stem cells through the Wnt/β-catenin signaling pathway
Authors: Liu Bihan , Wang Shanshan , Sun Rui , Ding Huiguo , Li Lei 
Published: February 2023

Abstract
Background
LCSCs is a key factor in the occurrence and development of HCC. Hydrogen sulphide (H2S) is the third gas signalling molecule after carbon monoxide (CO) and nitrogen monoxide (NO). Exogenous H2S has been shown to inhibit the progression of HCC by increasing apoptosis and autophagy. However, whether H2S can affect LCSCs in HCC microenvironment still remains poorly understand.
Methods
In vitro, Flow cytometry (FCM), imaging quantitive flow cytometry, RNA-sequencing, and Tumor sphere-forming assay were carried out in the HCC cells and LCSCs to investigate the effect of H2S on biological functions and Wnt/β-catenin signaling pathway. Data was analyzed using unpaired Student's t-test and nonlinear regression. P < 0.05 was considered to indicate a statistically significant difference.
Results
Based on the FCM, we found that exogenous H2S were able to induce HCC cells apoptosis and promote stemness in HCC cells. RNA-sequencing, imaging quantitive flow cytometry showed that 23 genes was regulated in NaHS group, and NaHS can activate the Wnt/β-catenin signaling pathway in HCC cell lines. Treatment with the Wnt/β-catenin signaling inhibitor ICRT3 alleviated the NaHS-induced stemness increase in HepG2 and Hep3B cells. Furthermore, the tumor sphere-forming assay show that the number of sphere-forming cells was significantly increased in NaHS treated group and was inhibited significantly in ICRT3 treated group.
Conclusion
Exogenous H2S increased the expression of CD133, CD44 and β-catenin and promoted the stemness in LCSCs by activating the Wnt/β-catenin signalling, which strongly clarify the relationship between Exogenous H2S and LCSC stemness and may provide theoretical guidance and potential therapeutic approaches for liver cancer.
Curtailing environmental impact assessment unlikely to speed implementation of natural resource management
Authors: Struthers Cory , Murenbeeld Kathryn , Williamson Matthew 
Published: February 2023

Abstract
Environmental impact assessment (EIA) is a central procedure that governments use to evaluate the merits and risks of natural resource management decisions and is often labeled red tape. Increasingly, decisionmakers from across the political spectrum are exempting EIA in order to expedite implementation of necessary actions for climate resilience and clean energy. Yet few studies have quantified the extent that EIA is the main barrier to efficient implementation of government priorities. We gather administrative data from the US Forest Service (USFS) on more than 4,500 silviculture, fuels, and invasive species management actions initiated 2009–2021 and use survival analysis to compare the time it takes agency offices to comply with the 1970 National Environmental Policy Act (NEPA) relative to other implementation tasks. Results indicate that for most actions the USFS takes as long or longer to award first contracts and rollout first on-the-ground activities than to complete NEPA processes, and that the NEPA process accounts for one-fifth of planned implementation time. Results call attention toward efficiency barriers that slow implementation after EIA and away from exemption strategies that deliver short-term political benefits at the expense of public trust and sound decision-making under a shifting climate.
Age and sex variation of SARS-CoV-2 cycle threshold value during prevalence of Omicron BA.5 sublineage
Authors: Pighi Laura , Henry Brandon M. , De Nitto Simone , Gianfilippi Gianluca , Salvagno Gian Luca , Lippi Giuseppe 
Published: February 2023

Abstract
Background: In this work we explore the possibility that age and sex may have an impact on the nasopharyngeal viral load of subjects infected by the SARS-CoV-2 Omicron BA.5 sublineage.
Methods: The study population was a cohort of outpatients who initially presented to the COVID-19 diagnostic facility of Hospital Pederzoli (Peschiera del Garda, Verona, Italy) ≤3 days from onset of suggestive symptoms, and subsequently tested positive for SARS-CoV-2. The study took place when the local prevalence of SARS-CoV-2 Omicron BA.5 sublineage was >90%. A nasopharyngeal swab was collected and immediately transported to the local diagnostic laboratory, where it was used for SARS-CoV-2 testing with Xpert Xpress SARS-CoV-2.
Results: The final study population consisted in 112 subjects, with median age of 45 years (77 females). In univariate analysis, no significant correlation was found between Ct value and sex (r= 0.02; p=0.817) or age (r= 0.02; p=0.798). the mean Ct value did not differ between males (24.7; IQR, 21.7-29.3) and females (25.2; IQR, 21.1-30.4; p=0.389). In multivariate analysis, the mean Ct values was not associated with sex (p=0.779) or age (p=0.979).
Conclusion: The viral load in patients with SARS-CoV-2 Omicron BA.5 infection is not dependent on age and sex.
Spectral simulation and materials design for camouflage textiles coloration against materials of multidimensional combat backgrounds in visible and near infrared spectrum
Authors: Hossain Md. Anowar 
Published: February 2023

Abstract

Visible and near infrared spectra of “sixteen materials for textile coloration/ finishing/patterning” such as titanium dioxide, calcium oxide, aluminum, tin metal, tin oxide, iron powder, boron carbide, magnesium powder, carbon black pigment, titanium carbide, isolan black 2S LDN, isolan orange, telon blue A 2R, telon red A 2R, telon violet 3R and telon yellow A 2R; and ‘nine materials of combat backgrounds (CBs) such as dry leaves, green leaves, tree bark-woodland CB; water-marine CB; sand-desertland CB; stone-stoneland CB; snow-snowland CB; sky CB and ice-iceland CB (DGTWSIB) are obtained by Fourier transform infrared spectrophotometry and colorflex EZ spectrophotometer. A method of ‘Monte Carlo cross validation’ was applied for spectral simulation in visible and near infrared spectrums through experimental data information. The characterized reflection spectra of zero reflection (ZR), low reflection (LR), high reflection (HR) and HR-LR (HLR) materials are coalesced and simulated for camouflage materials design and textile applications against multidimensional CBs, DGTWSIB. The reflections of aluminium, titanium dioxide, calcium oxide, tin metal, tin oxide and iron powder are irradiated as HR materials. Oppositely boron carbide, magnesium powder, carbon black pigment and titanium carbide are illuminated as LR materials. Consequently, the mixing principle of HR and LR materials are also classified as HLR materials. Spectral properties of CB materials are also depicted as ZR materials against selected CBs. Spectral signal of ZR, LR, HR and HLR materials are identified as more expedient camouflage materials for concealment of target signature than six selected synthetic dyes such as Isolan Black 2S LDN, Isolan Orange, Telon Blue A 2R, Telon Red A 2R, Telon Violet 3R and Telon Yellow A 2R. The reflection spectra of ZR, LR, HR and HLR materials are simulated and correlated against DGTWSIB in visible and NIR spectrums. Simulated spectral signals are considered for camouflage materials design and camouflage textiles formulation against DGTWSIB combat location, the CBs are mostly practiced by defence professional. Furthermore, the reflection principle of camouflage textiles coloration/finishing/patterning has been accumulated under spectral signal of DGTWSIB, camouflage materials and synthetic dyes, synthetic dye-metal and synthetic dye-pigment combination. Therefore depth analysis and graphical results of ZR, LR, HR and HLR materials are the potential findings for selection of camouflage materials, right development of camouflage textile products and camouflage assessment of hyperspectral imaging for defence protection in entire spectrums of UV-visible-IR. This optical parameters of ZR, LR, HR and HLR materials have also applications to the materials community of multidimesional branches of material research.
Spatial distribution and seasonal profile of parasitic contamination in sewage water samples from the city of Santana do Ipanema, Northeast region of Brazil
Authors: Lima P. D. , Alencar Vitória Jordana Bezerra , Machado João Paulo Vieira , Júnior Aécio Prado Lima , Lima Maria Wilma da Silva , Silva Laryssa Oliveira , Gomes Sheilla Conceição , Bispo Martha Rejane Souza , Silva Ádrian Cabral , Bezerra Letícia Pereira , Cirilo Tatyane Martins , Paz Wandklebson Silva da , Santos Israel Gomes de Amorim , Ramos Rosália Elen Santos 
Published: February 2023

Abstract
Wastewater released into the environment without proper treatment contributes to the high prevalence of parasitic infections. We aimed to investigate the occurrence of parasites in sewage waters in both rainy and dry seasons in the city of Santana do Ipanema, Brazil. This is a descriptive cross-sectional study carried out between the months of June, July (rainy season) and October (dry season) of 2021. A total of 50 streets were selected by a sample calculation in the Epi Info™ program. From each street, two wastewater samples were collected in both climatic periods. In the laboratory, the water samples were submitted to the Bailenger method with some adaptations and analyzed under an optic microscope. In the rainy season, we detected Strongyloides stercoralis (87.6%; 134), Strongylus spp. (4.6%; 7), hookworms (2.0%; 3) and Iodamoeba butschlii (62.5%; 5), and in the dry season, it was detected S. stercoralis (55.0%; 71), Metastrongylids (19.4%; 25), hookworms (12.4%; 16) and I. butschlii (76.5%; 13). The spatial analysis has shown that most hotspots were associated with S. stercoralis, mainly during periods of intense rainfall and close to river areas. Thus, the city population is subject to infections, especially by helminths regardless of seasonality.
Role of miRNA-99a-5p in Modulating the Function of Hepatocellular
Carcinoma Cells: Bioinformatics Analysis and In Vitro Assay
Authors: Zhang Jia-Ning , Wei Feng , Zheng Bin Bin , Tang Liang , Chen Feng-Yuan 
Published: February 2023

Aim:
This study aimed to investigate the biological functions of miRNAs in hepatobiliary
tumors as the focus of targeted therapy research.

Background:
Hepatobiliary tumors are among the leading causes of cancer-related deaths worldwide.
Many microRNAs (miRNAs) play an important regulatory role in tumor progression. Our study aims
to explore some biologically functional miRNAs from different datasets of hepatobiliary tumors for
disease diagnosis or treatment.

Objective:
In this study, we tried to filter out differentially expressed miRNAs in different tumor datasets
from the GEO database.

Methods:
In this study, we first perform analyses in different GEO data sets. After taking the intersection,
the initial scope is limited to several differential RNAs. Then, combined with the existing research
results from Kaplan-Meier survival analysis and literature, the candidate molecule was finally
identified to be studied. Furthermore, the biological characteristics analysis of the candidate molecule
was performed on the basis of Cancermirnome online tool, including expression levels in tumors,
KEGG and GO analysis, ROC analysis, and target gene prediction. Furthermore, the effect of the candidate
molecule on the biological functions of liver cancer was verified by in vitro assay.

Results:
The preliminary analysis of bioinformatics shows that 16 differentially expressed miRNAs
may play an important role in HCC or ICC. Ultimately, we identified miRNA-99a-5p as the only molecule
to study. The results showed that miRNA-99a-5p is abnormally expressed in many tumors, and
in liver cancer, its level of expression in tumor tissue is significantly lower than that in normal tissue.
Then, the KEGG and GO analysis found that it functions in multiple pathways. At the same time, the
ROC analysis found that it showed great potential for prognostic prediction in HCC and we also predicted
that RUNDC3B is the most likely target to which it binds. Finally, the experimental results of
overexpression and knockdown confirmed that miRNA-99a-5p could inhibit cell proliferation in HCC,
which also suggested that it may be an important tumor suppressor in HCC.

Conclusion:
MiRNA-99a-5p was negatively correlated with HCC progression and could act as a novel
therapeutic target for HCC.
Role of Gut Microbiome in Atherosclerosis: Molecular and Therapeutic
Aspects
Authors: Salazar Juan , Morillo Valery , Suárez María K , Castro Ana , Ramírez Paola , Rojas Milagros , Añez Roberto , D'Marco Luis , Chacín-González Maricarmen , Bermúdez Valmore 
Published: February 2023

Abstract:
Atherosclerosis is one of the most relevant and prevalent cardiovascular diseases of our
time. It is one of the pathological entities that increases the morbidity and mortality index in the
adult population. Pathophysiological connections have been observed between atherosclerosis and
the gut microbiome (GM), represented by a group of microorganisms that are present in the gut.
These microorganisms are vital for metabolic homeostasis in humans. Recently, direct and indirect
mechanisms through which GM can affect the development of atherosclerosis have been studied.
This has led to research into the possible modulation of GM and metabolites as a new target in the
prevention and treatment of atherosclerosis. The goal of this review is to analyze the physiopathological
mechanisms linking GM and atherosclerosis that have been described so far. We also aim
to summarize the recent studies that propose GM as a potential target in atherosclerosis management.
Multi-Sectoral Involvement in the National Action Plan on Anti-Microbial Resistance in Bangladesh: A Qualitative Study
Authors: Khandker Salamat , Thomas Shruthi Anna , Hossain Syed Billal , Akhter Aivee , Bakibillah Abul Hasan , Muhammad Faisal , Mathew Philip , Ahmad Sheikh Akhtar 
Published: February 2023

Background
To combat anti-microbial resistance in Bangladesh, a national action plan is designed with the involvement of multiple sectors.

Methods
Sixteen participants were interviewed virtually due to the COVID-19 pandemic. Semi-structured, pre-tested questionnaires were used for data collection using the Key-Informant Interview (KII) technique. Qualitative and quantitative analysis was carried out of relevant information.

Results
Bangladesh has no specific monitoring body for antibiotic prescription, and professionals do not follow national guidelines. Most healthcare settings don't have any protocol for Infection Prevention and Control (IPC), Standard Operating Procedure (SOP), and monitoring facilities. The main factor of Anti-Microbial Resistance (AMR) is the availability and accessibility of antibiotics and the dealers who supply antibiotics. Patients consume antibiotics without consulting any qualified doctor. The COVID-19 pandemic and the occurrence of AMR have escalated the use of antibiotics. Abuse of antibiotics in the animal industry is a worrying trend. The Bangladesh National Action Plan 2017 on AMR (BNAP), however, persuaded the top court to issue a ruling restricting the sale of antibiotics without a prescription and to establish an antibiotic surveillance system across the nation. The BNAP on AMR policy has been more effectively implemented thanks to multi-sectoral cooperation.

Conclusion
To monitor current facilities and spot trends in antibiotic resistance, a multi-sectoral regulatory organization specifically focused on AMR is desperately needed.