bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘I was eager to do anything I could to improve the situation’: A qualitative study of patients’ experiences and views of prehabilitation for ovarian cancer surgery
Authors: Saggu Rhia Kaur , Shaw Clare , Hughes Cathy , Lagergren Pernilla , Butler John , McGregor Alison H , Ghaem-Maghami Sadaf , Wells Mary 
Published: January 2023

Abstract
Background: Prehabilitation has shown promise in improving post-operative outcomes for several solid tumour groups. However, prehabilitation programmes are not widely established. Patients with advanced ovarian cancer experience life changing debulking surgery and could benefit from prehabilitation. This study aims to explore the views, experiences, facilitators and barriers surrounding prehabilitation in a demographically diverse cohort of advanced ovarian cancer patients. This would help to inform an acceptable patient-centred working programme model for a diverse group of patients.
Methods: Purposive, maximum variation sampling was used to recruit a diverse sample of women, due to undergo or following primary debulking surgery for advanced ovarian cancer, from two cancer centres in London. Semi-structured interviews were either conducted face to face or by telephone. All recordings were transcribed verbatim and analysed using thematic analysis.
Results: Twenty-one participants were interviewed. Twelve were prehabilitation ‘naïve’ and nine had participated in the Marsden Integrated Lifestyle and Exercise programme (MILE). The age range was 46-76 years and 8/21 participants were of Black, Asian or Mixed heritage. Factors influencing engagement with prehabilitation can be categorised under four major emerging themes (1) Mindset (2) Actual preparation (3) Support system (4) Delivery of prehabilitation.
Discussion/Conclusion: Patients with ovarian cancer welcome the concept of prehabilitation, however a blanket approach is not suitable to meet the needs of a demographically diverse cohort. The components of prehabilitation must be tailored to individual needs, with attention to existing mindset and support systems, building on preparations that women are already making for surgery and offering flexible delivery options.
Research on visualization of concrete crack depth detection based on tracer electromagnetic method
Authors: Wang Yulei , Tang Lei , Zhang Shenghang , Jia Yu , Tian Hui , Liang Jiahui 
Published: January 2023

Abstract
The invisibility of concrete cracks due to their expansion to the inside has resulted in no effective crack depth detection technology to date. The traditional concrete crack depth detection methods have the problems of insufficient accuracy and low detection efficiency. To solve these problems, this paper proposes a new concrete crack depth detection method based on the combination of tracer and hand-held radar. The method uses tracer diffusion to the bottom of the crack propagation, and then maps the location of the air-tracer interface by radar waves, thus visualizing the crack depth information. This paper elaborates the detection principle of this new method, conducts numerical analysis and simulation to verify the feasibility, makes standard concrete crack specimen, carries out relevant experimental studies, selects distilled water and saturated NaCl solution as tracer to verify the feasibility of the method, and compares and analyzes the detection effect of these two tracers by comparing the experimental test data with the actual depth of the specimen.
Efficient hip joint distraction using the AO large femoral distractor in treating acetabular fractures associated with marginal impaction and intraarticular incarcerated fragments
Authors: Badran Mahmoud , Khalifa Ahmed A. , Fergany Ali , Ibrahim Bahaaeldin , Moustafa Mohamed , Adem Ephrem , Gilyen Botond , Farouk Osama 
Published: January 2023

Abstract
Purpose
The results after acetabular fracture are primarily related to the quality of articular reduction. Using the AO large femoral distractor, incarcerated fragments can be easily removed, and elevation of marginally impacted fragments can be done under direct visualization without further re-dislocating the joint. The current study aimed to evaluate our early results of using the AO large femoral distractor as an assisting tool during ORIF of acetabular fractures associated with marginal impaction or intraarticular incarcerated fragments.
Methods
Eighteen patients were included in this retrospective case series study diagnosed with an acetabular fracture associated with either marginal impaction injury or an intraarticular incarcerated fragment. On a usual operative table, all patients were operated upon in a prone position through the Kocher Langenbeck approach. The AO large femoral distractor was used to facilitate hip joint distraction. Postoperative fracture reduction and joint clearance were assessed in the immediate postoperative CT scans.
Results
The average age of the patients was 30 ± 8.2 years; 13 (72.2%) were males. All cases had a posterior wall fracture, and it was associated with transverse fractures, posterior column fractures, and T-type fractures in five (27.8%), two (11.1%), and one (5.6%) patients, respectively. Intraarticular incarcerated fragments were present in 13 (72.2%) cases and marginal impaction in five (27.8%). Fracture reduction measured on the postoperative CT scans showed an anatomical reduction in 14 (77.8%) patients, imperfect in four (22.2%), and complete clearance of the hip joint of any incarcerated fragments.
Conclusion
The use of the AO large femoral distractor is a reliable and reproducible technique that can be applied to assist removal of incarcerated intraarticular fragments and to ease the reduction of marginally impacted injuries associated with acetabular fractures
The NLRP3 molecule influences the therapeutic effects of mesenchymal stem cells through reprogramming energy metabolism
Authors: Chen Jingrou , Xie Shujuan , Qiu Dongbo , Xie Maosheng , Wu Mengye , Li Xiaoping , Zhang Xiaoran , Wu Qili , Xiong Yi , Wu Changyou , Ren Jie , Peng Yanwen 
Published: January 2023

Abstract
Background
Numerous studies have demonstrated that NLRP3 is involved in the pathogenesis of inflammatory bowel disease (IBD). Mesenchymal stem cells (MSCs) have been highlighted as new candidates for treating IBD based on their immunomodulatory properties, including regulation of the NLRP3 inflammasome. However, the role of NLRP3 in shaping the immunoregulatory function of MSCs remains unclear. In this study, we demonstrated that NLRP3 influences the therapeutic effect of MSCs on colitis in an inflammasome-independent manner.
Methods
MSCs were isolated from the femurs of male C57/B6 mice or Nlrp3 KO mice, identified by osteogenic adipogenic differentiation assay and flow cytometry. In vitro, WT and Nlrp3 KO MSCs were treated with LPS, then the supernatant was collected; WT MSCs were stimulated with different concentrations of NLRP3-specific inhibitor MCC950, and the level of IL-10 was detected in the supernatant; Detect gene expression levels by RNA sequencing; Use Seahorse to detect cellular oxidative phosphorylation (OXPHOS) and glycolysis levels; The expression of glucose transporter 1 (Glut1) in MSCs was detected by Western blot; WT MSCs were stimulated with different concentrations of Glut1 inhibitor BAY876, and the level of IL-10 was detected. In vivo, DSS-induced colitis was treated with WT or Nlrp3 KO MSCs, body weight changes were monitored daily, measure colon length and collect colons for further evaluation; Then DSS-induced colitis was treated with pretreated MSCs, such as BAY876 pretreated WT MSCs or Glut1-overexpressed Nlrp3 KO MSCs (OE-Glut1), then the experimental operation is as above.
Results
Nlrp3 knockout did not alter MSC phenotypes, but promoted osteogenic differentiation. Further investigation indicated that deletion of Nlrp3reduced the production of the IL-10 in MSCs, leading to an impaired protective effect on DSS-induced colitis. Mechanistically, the deficiency of Nlrp3 downregulated Glut1 expression and glycolysis activation in MSCs, which reduced IL-10 production. Subsequently, overexpressing Glut1 in Nlrp3 KO MSCs recovered their therapeutic effect dampened due to Nlrp3 deletion.
Conclusions
These results improve the current understanding of the molecular mechanisms underlying the therapeutic effectiveness of MSCs and provide a basis for optimizing MSC-based therapeutic strategies for immune-mediated diseases.
Enzymatic and biochemical responses to high temperatures and drought stress during the reproductive stage in lentil (Lens Culinaris Medik.)
Authors: Haddad Noureddine El , En-nahli Youness , Choukri Hasnae , Aloui Khawla , Mentag Rachid , El-Baouchi Adil , Hejjaoui Kamal , Karthika Rajendran , Smouni Abdelaziz , Maalouf Fouad , Kumar Shiv 
Published: January 2023

Abstract
Increasing temperatures and drought stress are two major factors limiting lentil (Lens culinaris Medik.) productivity, particularly during reproductive stage. The current research aimed to determine the response of lentil genotypes with varying heat and drought sensitivity. For this purpose, twelve genotypes were evaluated under high temperature stress (> 32°C), while seven genotypes were investigated under drought stress conditions during the reproductive stage (75% field capacity). Under stress conditions, the results revealed significant variation in enzymatic responses between tolerant and susceptible genotypes. Under high temperature and drought stress, tolerant genotypes accumulated higher concentrations of catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD), whereas susceptible genotypes had low enzymatic responses. Proline content (PC) increased significantly with high temperature and drought stress in tolerant genotypes, but not in susceptible genotypes. Because of high temperature and drought stress, the concentrations of total antioxidant activity (TAA), total phenolic content (TPC), tannins (TC), and total flavonoids (TFC) increased significantly in tolerant genotypes compared to susceptible genotypes. Furthermore, when tolerant genotypes were stressed, there were significant increases in total soluble sugars (TSS) and reducing sugars (RS) compared to normal conditions. Our results indicated that SOD had significant positive correlation with TAA, TC, PC and RS under high temperature, while it was correlated with CAT, TC, TAA under drought conditions. Highly significant correlation was also observed between CAT and AOP under both stressed conditions. Tolerant genotypes that responded better to high temperature and drought stress may be able to maintain physiological processes and generate high yield potential.
Neutrophil elastase as a potential biomarker related to the prognosis of gastric cancer and immune cell infiltration in the tumor immune microenvironment
Authors: Jia Wangqiang , Luo Qianwen , Wu Jiang , Shi Yuanchao , Guan Quanlin 
Published: January 2023

Abstract
Purpose: Exploring biomarkers interrelated the tumor immune microenvironment (TME) provides novel ideas for predicting the prognosis of gastric cancer (GC) and developing new treatment strategies.
Methods: We analyzed the differential gene expression levels between the high and low StromalScore and ImmuneScore groups. Neutrophil elastase (ELANE) was evaluated as a potential biomarker by conducting intersection analysis of the protein-protein interaction network (PPI) and univariate Cox regression analysis. Its prognostic value was evaluated using Kaplan-Meier (K-M) survival curves and multivariate Cox regression analysis and its potential biological molecular mechanism was examined by gene set enrichment analysis (GSEA). We applied the CIBERSORT computing method to analyze the relationship between ELANE and tumor immune-infiltrating cells (TIICs).
Results: K-M survival curve showed that higher ELANE expression was closely related to shorter overall survival. The Cox regression analysis indicated that the high expression of ELANE was an independent prognostic risk factor in patients with GC. The GSEA revealed that genes in the ELANE high-expression group were involved in the signaling pathways regulating immune response; genes in the ELANE low-expression group were involved in the signaling pathways that regulate metabolism. ELANE might be participate in the change of TME from immunodominant to metabolically dominant and its expression was closely related to tumor mutation burden and multiple TIICs.
Conclusion: ELANE is a potential biomarker for predicting the GC patients’ survival and prognosis. It influences the tumor immune cell infiltration in the TME, and affects the TME to maintain their immune status.
Geochemical signals of high-temperature fluid–rock interactions in a mélange unit, Japan: Implications for geochemical reactions along major subduction-related faults
Authors: Shimamoto Ayana , Ishikawa Tsuyoshi , Hirono Tetsuro 
Published: January 2023

Abstract
To understand the slip behavior and characteristics of major faults within subduction-related mélange units, we performed microscopic observations and mineralogical and chemical analyses of a primary slip zone and its surrounding host rocks in the Hidakagawa Group of the Shimanto accretionary complex, Kii Peninsula, Japan. The primary slip zone is characterized by the development of composite planar fabrics such as R1 shear planes and abundant sub-micrometer-sized albite particles. Concentrations of fluid-mobile elements such as Sr, Rb, and Cs in the slip zone were anomalous compared to those in the host rocks. Geochemical modeling suggests that fluid–rock interactions in the slip zone occurred at 250–300°C and were accompanied by albitization. The presence of such high-temperature fluids in slip zones could drastically weaken faults through thermal pressurization, perhaps promoting the occurrence of tsunamigenic earthquakes.
Mesenchymal stem cells reduced the extracellular mitochondrial DNA-mediated TLR9 activation in neonatal hyperoxia-induced lung injury
Authors: Kim Young Eun , Ahn So Yoon , Sung Se In , Yang Misun , Sung Dong Kyung , Park Won Soon , Chang Yun Sil 
Published: January 2023

Abstract
Mitochondrial DNA (mtDNA) released from the dead or injured cells can activate inflammation and mesenchymal stem cell (MSC) transplantation can reduce inflammatory injury. However, it has not been tested whether the release of mtDNA can be reduced by MSC transplantation. We hypothesized that level of extracellular mtDNA would be increased after hyperoxia-induced lung injury but reduced after lung injury attenuation by MSC therapy in our newborn rat model. In an in-vitro study using a rat lung epithelial L2 cell line, we found that the level of extracellular mtDNA was significantly increased with H2O2-induced cell death, but was reduced after MSC co-incubation. In an in-vivo study, we confirmed that the levels of cell death, extracellular mtDNA, and inflammatory cytokines were significantly increased in hyperoxic newborn rat lungs, but reduced after MSC transplantation. The levels of extracellular mtDNA were significantly positively correlated with the levels of the inflammatory cytokines. The TLR9/MyD88/NFκB pathway, which is activated by binding with mtDNA, was also significantly up-regulated but down-regulated after MSC transplantation. In intubated neonates, we found a significant positive correlation between inflammatory cytokines and extracellular mtDNA. The levels of inflammatory cytokines and extracellular mtDNA changed over time in a similar pattern in transtracheal aspirate samples from intubated neonates. In conclusion, increased levels of extracellular mtDNA were associated with increased inflammation in hyperoxia-induced lung injury, and attenuated lung inflammation by MSC therapy was associated with reduced levels of extracellular mtDNA.
Characteristics of patients with COVID-19 who have deteriorating chest X-ray findings within 48 hours: a retrospective cohort study
Authors: Kusumoto Tatsuya , Chubachi Shotaro , Namkoong Ho , Tanaka Hiromu , Lee Ho , Otake Shiro , Nakagawara Kensuke , Fukushima Takahiro , Morita Atsuho , Watase Mayuko , Asakura Takanori , Masaki Katunori , Kamata Hirofumi , Ishii Makoto , Hasegawa Naoki , Harada Norihiro , Ueda Tetsuya , Ueda Soichiro , Ishiguro Takashi , Arimura Ken , Saito Fukuki , Yoshiyama Takashi , Nakano Yasushi , Mutoh Yoshikazu , Suzuki Yusuke , Edahiro Ryuya , Murakami Koji , Sato Yasunori , Okada Yukinori , Koike Ryuji , Kitagawa Yuko , Tokunaga Katsushi , Kimura Akinori , Imoto Seiya , Miyano Satoru , Ogawa Seishi , Kanai Takanori , Fukunaga Koichi 
Published: January 2023

Abstract
Background
The severity of chest X-ray (CXR) findings is a prognostic factor in patients with coronavirus disease 2019 (COVID-19). However, the prognostic impact of deterioration of CXR findings and the clinical characteristics of patients with worsening CXR findings remain unclear. We aimed to investigate the clinical and genetic characteristics, as well as the prognosis, of patients with worsening CXR findings during early hospitalisation.
Methods
We retrospectively included 1656 consecutive Japanese patients with COVID-19 recruited through the Japan COVID-19 Task Force. Rapid deterioration of CXR findings was defined as increased pulmonary infiltrates in ≥ 50% of the lung fields within 48 h of admission.
Results
Rapid deterioration of CXR findings was an independent risk factor for death, most severe illness, tracheal intubation, and intensive care unit admission. The presence of consolidation on CXR, comorbid cardiovascular and chronic obstructive pulmonary diseases; high body temperature (≥ 37.7°C); and increased levels of serum aspartate aminotransferase (≥ 30 IU/L), potassium (≥ 4.3 mEq/L), and C-reactive protein (≥ 2.53 mg/dL) were independent risk factors for rapid deterioration of CXR findings. The risk variant at the ABO locus (rs529565-C) was associated with rapid deterioration of CXR findings in all patients with COVID-19. Further, the population-specific risk variant at the DOCK2 locus (rs60200309-A) was nominally associated with rapid deterioration of CXR findings in patients aged < 65 years.
Conclusions
This study revealed the clinical features, genetic features, and risk factors for rapid deterioration of CXR findings in patients with COVID-19. Rapid deterioration of CXR findings is a poor prognostic factor for patients with COVID-19.
CFD Analysis of Different edge shape of RCC Chimney using M25 Grade of Concrete
Authors: pandey Swati , Verma Priyanka , agrawal Gaurav kumar 
Published: January 2023

Abstract
The purpose of chimney is to vent hot flue gases from furnaces/boilers. The structure is vertical and mostly of circular or rectangular shape. The objective of current research to investigate the wind load effect on chimney using Fluid Structure Interaction (FSI) studies. The FSI investigation is conducted on chimney using ANSYS simulation package. The induced pressure, drag force and velocity fields are evaluated across the chimney with different shapes i.e., sharp edged, chamfered, and filleted edged shapes. The FSI study is conducted on chimney with M25 concrete material. The drag force values are obtained for each design and minimum drag force is obtained for filleted design of chimney. The drag force induced on filleted chimney is 3.33 times lower than generic design. The drag force induced on chamfered chimney is 16.39% lower than generic design The lowest induced pressure is observed on filleted chimney and highest induced pressure is observed on generic design.
Resource Allocation Methods in Vanets:a Systematic Literature Review
Authors: Trombly Andrew , Alsmadi Izzat 
Published: January 2023

Abstract
Autonomous vehicles take on a more prominent part of our every day life activities. As the number of vehicles grows so does the need for resources to ensure safe and consistent operations of these vehicles. Today's networks and power grid are already used heavily just supplying resources for our day to day lives and leisure. The continuous increase in demand for such resources drives the need for more advanced resource management tools and techniques and more precise resource allocation schemes. This paper is a systematic literature review on the current methods and research trends for resource allocation in vehicular networks. The purpose of this review is to understand current research trends and motivations. Our hope is to provide a comparative analysis of resource allocation solutions, research trends and also challenges in vehicular networks.
Randomized Double-blind Placebo-controlled Study of Salivary Substitute With Enzimatic System for Xerostomia in Patient Irradiated in Head and Neck Region
Authors: Porangaba Leticia Pacheco , Garcia Flavio de Melo , Rabelo Ana Paula Alvarenga Antonio , Andrade Amanda Puche , Alves Fabio de Abreu , Pellizzon Antonio Cassio Assis , Jaguar Graziella Chagas 
Published: January 2023

Abstract
To compared whether the use of Bioxtra Spray® clinically reduces xerostomia intensity, impacting on the quality of life of patients who received radiotherapy in the head and neck region. A total of 40 patients were allocated in: Bioxtra Spray® group (n = 21) and Placebo arm (n = 19). Patients used both products three times a day for 30 days. For the analysis, xerostomia grade, unstimulated (UWS) and stimulated (SWS) salivary flow rate and quality of life through the University of Washington Quality of Life Questionnaire, validated in Portuguese (UW-QoL) were performed in two phases: Phase 1 (before the use of both products); Phase 2 (after 30 days of using both products). All clinical data were collected from medical records. Regarding salivary flow rate, it was observed that after 30 days of use, the Bioxtra Spray® group showed UWS and SWS significantly when compared to the Placebo arm (p < 0.05). Analyzing the Bioxtra Spray® Group, they showed improvement in xerostomia complaints compared to 30 days after using the product. Dispite this, we did not observed statistical differences (p > 0.05).Regarding quality of life, no significant differences were observed in relation to the UW-QoL saliva domain between the groups in the two phases of the study (p > 0.05). We concluded that Bioxtra Spray® may be effective in reducing radio-induced xerostomia symptoms. However, further research is necessary to evaluate the efficacy of BioXtra on oral health.
Trial registration number: RBR-9sdf3k
Date of trial registration submission: 10th June 2019