bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract P6-13-01: EPITHELIAL-MESENCHYMAL TRANSITION AND TUMOR STEM CELLS NETWORK IDENTIFICATION IN BREAST CARCINOMAS: IN SILICO STUDY
Authors: Dybal Vanessa , Cavalcante Bruno R. , Rocha Gisele V. , Gurgel Clarissa 
Published: March 2023

Abstract
INTRODUCTION: Breast cancer (BC) is the most prevalent malignant tumor in the female population, except for skin tumors. Despite therapeutic advances, over 20% of patients with localized disease will relapse. The research of epithelial-mesenchymal transition (EMT), tumor stem cells (TSC), and the biological mechanisms related to refractoriness and metastasis development is, therefore, an opportunity for new therapeutic strategies and better results. OBJECTIVES: To identify breast cancer cellular markers related to EMT and TSC according to their histological subtypes, stage and to analyze their relationship with clinical outcomes. METHODS: After selecting a public breast cancer patients database with interactome, we identified differentially expressed genes, their associated processes, coexpression networks and interactions with pathways related to the stem phenotype and epithelial-mesenchymal transition. The characterization of key genes and the correlation with histological subtypes and clinical outcome allowed us to determine a group of genes as potential breast cancer EMT/TSC prognostic markers. RESULTS: In the 989 patients studied, 1033 differentially expressed genes (DEGs) were found, categorized according to histological subtype (hormone receptor positive, HER2 positive, triple negative) and stage IV. Seven communities of gene coexpression were found, with the gray community showing greater interaction with hormone positive tumors, the green community with HER2 positive, and the turquoise community with the triple negative one. The hormone positive disease was related to extracellular matrix processes and neuronal communication, the HER2 positive to the extracellular matrix interaction and the triple negative tumors to mitotic processes. Investigation networks with EMT and TSC related genes demonstrated a strong correlation with HER2-positive and triple-negative tumors; being eight genes in HER2 positive subtypes correlated with survival (SYNDIG1, ​​COL10A1, SLC24A2, LINC00922, KLKP1, MMP11 and ECM2); and one of the hormone positive subtype (ITIH5). ECM2 was highlighted in terms of EMT/TSC connectivity and survival. CONCLUSION:The EMT/TSC processes are significant in the various subtypes of breast cancer and impact on survival, especially in the HER2 positive subtype.
Citation Format: Vanessa Dybal, Bruno R. Cavalcante, Gisele V. Rocha, Clarissa Gurgel. EPITHELIAL-MESENCHYMAL TRANSITION AND TUMOR STEM CELLS NETWORK IDENTIFICATION IN BREAST CARCINOMAS: IN SILICO STUDY. [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr P6-13-01.
Abstract PD15-08: PD15-08 Brazilian Randomized Study - BREAST-MRI Trial - Impact of Preoperative Magnetic Resonance in the Evaluation for Breast Cancer Conservative Surgery: Local recurrence and surgical outcomes
Authors: Mota Bruna S. , Reis Yedda N. , Barros Nestor , Cardoso Natalia , Mota Rosa s. , Shimizu Carlos , Tucunduva Tatiana , Gonçalves Rodrigo , Doria Maira T. , Ferreira Vera , Ricci Marcos , Trinconi Angela , Riera Rachel , Baracat Edmund C. , Soares José Maria , Filassi Jose Roberto 
Published: March 2023

Abstract
Introduction: Breast magnetic resonance imaging (MRI) has high sensitivity in detecting invasive neoplasms. However, controversy remains as to whether preoperative staging with breast MRI impacts surgical outcomes and local recurrence. Materials and Methods: BREAST-MRI is a randomized, open-label trial including female breast cancer patients older than 18 years old, with stage 0-III disease, eligible for BCS. We performed a 1:1 stratified randomization by breast density according to ACR-BIRADS to divide patients into two groups; one in which preoperative MRI was used and the control group where the MRI was not used. The primary outcome was local relapse-free survival (LR). Secondary outcomes were overall survival (OS), repeat operation, and the proportion of patients whose surgical management was modified to mastectomy. Results: 524 were randomized, 257 included in the MRI group, and 267 in the control group. The baseline characteristics were similar between groups, except for chemotherapy use (table 1). The survival analysis showed a 6-year local recurrence-free survival was 99.2% in MRI group versus 98.9% in the control group, p=0.702, overall survival of 95.3% in the MRI group versus 96.3% in the control group, p=0.481. No difference was found in reoperation rates, 22 (8.7%) in the MRI group versus 23 (8.7%) in the control group (p= 0.85)(table2). Surgical management changed in 21 ipsilateral breasts in the MRI group; 21 (8.3%) had mastectomies versus 1 in the control group (p&lt; 0.01). Conclusion: Preoperative MRI evaluation increased the mastectomy rates by 8%. The use of preoperative MRI did not influence local relapse-free survival, overall survival, or reoperation rates Keywords: breast magnetic resonance imaging; breast cancer; conservative surgery; MRI accuracy, surgical outcomes, randomized clinical trial
Citation Format: Bruna S. Mota, Yedda N. Reis, Nestor Barros, Natalia Cardoso, Rosa s. Mota, Carlos Shimizu, Tatiana Tucunduva, Rodrigo Gonçalves, Maira T. Doria, Vera Ferreira, Marcos Ricci, Angela Trinconi, Rachel Riera, Edmund C. Baracat, José Maria Soares Jr, Jose Roberto Filassi. PD15-08 Brazilian Randomized Study - BREAST-MRI Trial - Impact of Preoperative Magnetic Resonance in the Evaluation for Breast Cancer Conservative Surgery: Local recurrence and surgical outcomes [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr PD15-08.
Abstract PD3-06: Robotic Stereotactic APBI for Early-Stage Breast Cancer: 2-year Outcomes of a Prospective Multi-Institutional Trial
Authors: Cantalino Jonathan M. , D’Ambrosio David , Hirsch Arica , Collins Brian , Danner Malika , Matsanka Dawn , Rudra Sonali , Suy Simeng , Collins Sean , Marin Monica Pernia , Good Michael , Feng Jing , Lamond John , Markiewicz Deborah , Lanciano Rachelle , Obayomi-Davies Olusola 
Published: March 2023

Abstract
Purpose: Outcomes following adjuvant accelerated partial breast irradiation (APBI) in select women with early-stage breast cancer are comparable to whole breast irradiation. Robotic stereotactic accelerated partial breast irradiation (RSAPBI) with fiducial tracking is an attractive treatment option, but limited outcomes data are available for this approach. We report 2-year outcomes for a prospective multi-institutional trial treating select women with RSAPBI. Materials and Methods: Post-menopausal women with DCIS and Stage IA breast cancer were treated over a five-year period extending from November 2015 to November 2020 and were followed for a minimum of 18 months. Treatments were delivered with a robotic radiosurgery system. Four gold fiducials were implanted around the lumpectomy cavity prior to the start of treatment for tumor bed delineation and target tracking. The CTV was defined as the lumpectomy cavity with a uniform 5-15 mm expansion confined to the breast tissue and the PTV was defined as the CTV with a 0-5 mm uniform expansion. The PTV was prescribed 30 Gy in 5 fractions. Disease status assessments were completed at 4 weeks, 3 months, 6 months, 12 months, 18 months, 24 months and yearly intervals thereafter for five years. Results: Eighty-one patients (median age 68 years) with hormone receptor-positive tumors were treated over a median 9 days (range, 5-15). Sixty-eight women had invasive ductal carcinoma (84%) and thirteen had DCIS (16%). The median treated PTV was 108 cm3 (IQR 66-156) and the median prescription isodose line was 81% (IQR 79-83). The median CTV expansion was 10 mm (range 5-10) and the median PTV expansion was 3 mm (range 0-5). At a median follow up of 2 years there was one new ipsilateral breast tumor diagnosed. There were no local, regional, or distant treatment failures. Conclusions: Two-year results suggest that RSAPBI with fiducial tracking is an effective technique for the adjuvant treatment of post-menopausal women with hormone receptor-positive early-stage breast cancer. Additional follow-up is planned to confirm this preliminary finding.
Citation Format: Jonathan M. Cantalino, David D’Ambrosio, Arica Hirsch, Brian Collins, Malika Danner, Dawn Matsanka, Sonali Rudra, Simeng Suy, Sean Collins, Monica Pernia Marin, Michael Good, Jing Feng, John Lamond, Deborah Markiewicz, Rachelle Lanciano, Olusola Obayomi-Davies. Robotic Stereotactic APBI for Early-Stage Breast Cancer: 2-year Outcomes of a Prospective Multi-Institutional Trial [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr PD3-06.
Abstract PD6-02: PD6-02 Health-Related Quality of Life Trajectories and Predictors among Older Breast Cancer Survivors: A SEER-MHOS analysis
Authors: Lee Eunkyung , Rupesh Sushantti , Ferdowsi Katia , Hines Robert , Loerzel Victoria 
Published: March 2023

Abstract
Health-related quality of life (HRQOL) is an important issue for breast cancer patients and clinicians in the treatment decision process. In addition, older women are more vulnerable due to pre-existing comorbidities and socioeconomic status. This study explored 10-year trajectories of HRQoL in older breast cancer survivors and their predictors using patient-reported outcomes data queried from the Surveillance, Epidemiology and End Results - Medicare Health Outcomes Survey (SEER-MHOS) data resources among Medicare beneficiaries. Older women diagnosed with breast cancer in 1998-2012 and who participated in the surveys before and at least once after diagnosis were included in the analysis. HRQOL was measured using SF-36/VR-12 questionnaire and summarized as Physical Component Summary (PCS) Score and Mental Component Summary (MCS) Score. Latent Class Growth Mixture Modeling was conducted to identify distinct groups of women with a similar trajectory of HRQOL. A total of 1089 women with breast cancer completed surveys an average of 2.6 times (range 2 - 19). The results showed that there were three latent classes of PCS trajectory: high-declining (46% of the sample), mid-declining (37%), and low-improving (17%). Two latent classes of MCS trajectory were identified: high-stable (76%) and low-declining (24%). The univariate analysis showed that age at diagnosis, body mass index, level of education, geographic region, tumor grade, tumor size, and the number of comorbidities were related to PCS and MCS scores. Multivariable multinomial logistic regression analysis identified the number of comorbidities as the most significant predictor for PCS score and level of education as the most significant predictor for MCS score. Future research needs to identify the most common comorbidities that influence HRQOL deterioration in older breast cancer survivors to develop interventions that better the physical HRQOL in patients. Also, interventions that target less educated, underserved patients to improve mental HRQOL.
Citation Format: Eunkyung Lee, Sushantti Rupesh, Katia Ferdowsi, Robert Hines, Victoria Loerzel. PD6-02 Health-Related Quality of Life Trajectories and Predictors among Older Breast Cancer Survivors: A SEER-MHOS analysis [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr PD6-02.
Abstract PD9-01: Stromal mediators of lymphocyte exclusion and dysfunction in triple negative breast cancer
Authors: Chen Julia , Wu Sunny , Ruan Travis , Slapetova Iveta , Millar Ewan , Lim Elgene , Swarbrick Alex 
Published: March 2023

Abstract
Triple negative breast cancer (TNBC) is the most aggressive breast cancer subtype with poor prognosis and limited treatment options. One of the mechanisms contributing to this is the ability of TNBC tumors to evade anti-tumour response, limiting the success of immune-checkpoint therapy. In recent studies of the tumor microenvironment, stromal cells have emerged as potential important mediators of lymphocyte function in TNBC. Multi-omics studies from our group and others have elucidated the heterogeneity of stromal cells and their interactions with immune cells in TNBC. However, the clinical relevance and functional characteristics of these relationships remain poorly explored. Our previous work revealed functionally distinct subpopulations of stromal cells in breast cancer: endothelial cells, myofibroblast-like cancer-associated fibroblasts (myCAFs), inflammatory-CAFs (iCAFs), and perivascular-like cells which resemble pericyte and smooth muscle cells in immature and differentiated states (imPVLs; dPVLs), respectively. Here we directly explored the clinical relevance of these stromal subpopulations and their association with immune evasion in a large independent TNBC cohort with long-term survival data. Using markers derived from our single-cell multi-omics studies, we performed multiplex immunofluorescence using the OPAL9 platform on tumor microarrays from 222 TNBC patients to mark myCAFs, iCAFs, dPVLs, endothelial cells, CD8 and/or PD1 positive T-cells. Digital imaging analysis (QuPath) revealed a significant negative correlation between the abundance of stromal cells and CD8 T cells. This cytotoxic T-cell exclusion is primarily driven by smooth muscle dPVLs. Parallel to this T-cell exclusion, our functional studies demonstrate that stromal cells suppress T-cell proliferation. Using multiple ex vivo co-culture models of primary TNBC CAFs and peripheral blood mononuclear cells (PBMCs) from healthy and matched donors, we show that CAFs suppress the proliferative capability of CD4 and CD8 T-cells. ScRNA-seq of these co-culture models demonstrate that CAF educated T cells are driven into a LAG3+ exhausted state enriched for canonical pathways of immunosuppressive cytokine signaling. Our findings suggest that manipulation of these stromal subpopulations could elicit a more effective immune response in a subset of patients through inhibiting T-cell dysfunction and exclusion.
Citation Format: Julia Chen, Sunny Wu, Travis Ruan, Iveta Slapetova, Ewan Millar, Elgene Lim, Alex Swarbrick. Stromal mediators of lymphocyte exclusion and dysfunction in triple negative breast cancer [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr PD9-01.
Abstract WS1-2: How to measure and improve drug adherence in clinical trials
Authors: Hershman Dawn 
Published: March 2023

Abstract
The past decade has seen a dramatic increase in the number of Food and Drug Administration approval of oral anti-cancer drugs (OACDs). In 2018 alone, OACDs comprised ten of the sixteen newly approved oncology therapeutics in the United States. Despite the clinical benefits and convenience of OACDs, these new oral options present potential challenges, including polypharmacy, and can result in increased toxicities, drug–drug interactions (DDIs), inappropriate consumption of medications, and medication non-adherence. Adverse drug events have the potential to affect quality of life, healthcare utilization, treatment response, and survival.
For the treatment of breast cancer, Endocrine therapies have remained a critical component of care for early stage and advance disease. Despite the proven efficacy adherence to therapy is suboptimal. Issues related to non-adherence are increasingly important, as analyses from prospective randomized trials show compliance to endocrine therapy is associated with improved disease-free survival. The reasons for non-adherence to hormonal therapy are multifactorial. Barriers to compliance include patient, physician, medication and system related variables and poor compliance is usually associated with a combination of these factors. Characteristics associated with non-adherence include very high and low age, minority race, being single, increased number of comorbidities, lack of knowledge about efficacy of AI therapy, history of non-adherence to other chronic medications, limited insurance status, and higher out of pocket costs. Side effects from hormonal therapy is the most common reason for early discontinuation. An understanding of factors that contribute to non-adherence and interventions that have been tested to improve adherence will be discussed.
Medication compliance has become a more salient issue in cancer care with the increased availability of oral antineoplastic therapies for breast cancer. The treatment of BC now routinely encompasses targeted therapies such as oral poly(ADP-ribose) polymerase (PARP) inhibitors and cyclin-dependent kinase 4 (CDK4) and CDK6 inhibitors, PI3 Kinase(Pi3K) Inhibitors, in addition to traditional ET. Furthermore, as advances in BC treatment have improved long-term prognosis, the management of other chronic conditions has become an essential component of BC survivorship care. It remains unclear how this increased oral medication burden on BC survivors affects compliance with both ET and other chronic medications. Issues related to adherence measurement will be discussed.
Citation Format: Dawn Hershman. How to measure and improve drug adherence in clinical trials [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr WS1-2.
Intraarterial Transplantation of Mitochondria After Ischemic Stroke Reduces Cerebral Infarction
Authors: Norat Pedro , Sokolowski Jennifer D. , Gorick Catherine M. , Soldozy Sauson , Kumar Jeyan S. , Chae Youngrok , Yagmurlu Kaan , Nilak Joelle , Sharifi Khadijeh A. , Walker Melanie , Levitt Michael R. , Klibanov Alexander L. , Yan Zhen , Price Richard J. , Tvrdik Petr , Kalani Mohammad Yashar S. 
Published: March 2023

BackgroundTransplantation of autologous mitochondria into ischemic tissue may mitigate injury caused by ischemia and reperfusion.MethodsUsing murine stroke models of middle cerebral artery occlusion, we sought to evaluate feasibility of delivery of viable mitochondria to ischemic brain parenchyma. We evaluated the effects of concurrent focused ultrasound activation of microbubbles, which serves to open the blood–brain barrier, on efficacy of delivery of mitochondria.ResultsFollowing intraarterial delivery, mitochondria distribute through the stroked hemisphere and integrate into neural and glial cells in the brain parenchyma. Consistent with functional integration in the ischemic tissue, the transplanted mitochondria elevate concentration of adenosine triphosphate in the stroked hemisphere, reduce infarct volume and increase cell viability. The addition of focused ultrasound leads to improved blood–brain barrier opening without hemorrhagic complications.ConclusionOur results have implications for the development of interventional strategies after ischemic stroke and suggest a novel potential modality of therapy after mechanical thrombectomy.
Problematizing Realism: Confronting Colonial Perspectives and Engaging Complexities of Landscape Through “Ghost Textures”
Authors: Ackerman Aidan , Koudelka Christopher , Hirsch Paul , Zaengle Owen 
Published: March 2023

Abstract
Realistic virtual reality visualizations of a physical place, whether historic or contemporary, can exude a sense of certainty about their accuracy. They can also evade complexity by excluding underrepresented narratives about place such as those held by indigenous and other marginalized communities. Through engaging with a framework of complexity, ethics, and humility, we propose a technique of immersive virtual storytelling (IVS) that foregrounds uncertainty through a combination of realistic material treatments and translucent, ephemeral “ghost textures.” By applying these treatments to two virtual three-dimensional models telling a pre- and post-construction story of a World War II-era rifle range, we were able to test the use of these treatments in exposing gaps in archival data. This highlighted the predominance of a colonial lens in guiding historic site visualization, raising a question of how other narratives might be included without conforming to a colonial framework.
Removal of the Vertical Structure Ambiguity in the Thermodynamic Retrieval from Multiple-Doppler Radar Synthesized Wind Fields
Authors: Liou Yu-Chieng , Teng Yung-Lin 
Published: March 2023

Abstract
It has been long recognized that in the retrieved thermodynamic fields using multiple-Doppler radar synthesized winds, an unknown constant exists on each horizontal level, leading to an ambiguity in the retrieved vertical structure. In this study, the traditional thermodynamic retrieval scheme is significantly improved by the implementation of the Equation of State (EoS) as an additional constraint. With this new formulation, the ambiguity of the vertical structure can be explicitly identified and removed from the retrieved three-dimensional thermodynamic fields. The only in situ independent observations needed to perform the correction are the pressure and temperature measurements taken at a single surface station. If data from multiple surface stations are available, a strategy is proposed to obtain a better estimate of the unknown constant. Experiments in this research were conducted under the observation system simulation experiment (OSSE) framework to demonstrate the validity of the new approach. Problems and possible solutions associated with using real datasets and potential future extended applications of this new method are discussed.
What makes cities happy? Factors contributing to life satisfaction in European cities
Authors: Castelli Chiara , d’Hombres Beatrice , Dominicis Laura de , Dijkstra Lewis , Montalto Valentina , Pontarollo Nicola 
Published: March 2023

The purpose of this study is to identify the main factors of city life satisfaction across Europe. Data come from the recent fifth survey on quality of life in European cities and cover 83 cities located in the European Union, the European Free Trade Association countries, the United Kingdom, the Western Balkan Region and Turkey. In addition to running classical econometric analysis, we quantify the relative importance of the various determinants of overall satisfaction with life in cities, thus offering novel insights to shape evidence-based urban policies. The results highlight that two main policy-relevant areas contribute to the satisfaction with city life: the presence of amenities, on the one hand, and the inclusiveness and safety feeling, on the other hand. Socio-economic characteristics are generally not relevant, with the exception of economic insecurity. JEL Codes: R10, R58, I31