bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA RBAT1 reduces chemosensitivity of cancer cells to carboplatin/paclitaxel by sponging miR‑27b in endometrial carcinoma
Authors: Fan Lingye , Wang Chunyan , Zhan Ping , Liu Yaofang 
Published: July 2023

Abstract
Background
A recent study reported the role of long non-coding RNA (lncRNA) RBAT1 in promoting the development of retinoblastoma and bladder cancer. However, its function in other cancers is unclear. We then studied the role of RBAT1 in endometrial carcinoma (EC).

Methods
The expression of RBAT1 and miR-27b in EC and paired non-tumor samples from advanced EC patients, as well as in plasma samples of EC patients and healthy controls were detected by RT-qPCR. The direct interaction between RBAT1 and miR-27b, and the subcellular location of RBAT1 were determined by RNA-RNA pulldown assay and subcellular fractionation assay, respectively.

Results
EC tissues showed increased expression levels of RBAT1 and decreased expression levels of miR-27b compared to that in non-tumor tissues. Moreover, EC patients showed higher plasma expression levels of RBAT1 and lower plasma expression levels of miR-27b compared to that in the controls. Drug-resistant (DR) patients showed higher expression levels of RBAT1 and lower expression levels of miR-27b in both EC tissues and plasma samples. RBAT1 was detected in both nuclear and cytoplasm and it directly interacted with miR-27b. RBAT1 and miR-27b did not affect the expression of each other. Upregulation of RBAT1 promoted the expression of multidrug-resistant-related protein (P-gp, MRP1, and BCRP). Overexpression of RBAT1 and inhibition of miR-27b promoted cell viability and impeded cell apoptosis and cell cycle arrest at G0-G1 phase, while knockdown of RBAT1 and overexpression of miR-27b inhibited cell viability and induced cell apoptosis and cell cycle arrest at G0-G1 phase. Moreover, miR-27b could abolish RBAT1-induced effects on cell viability, apoptosis and cell cycle.

Conclusion
RBAT1 may reduce the chemosensitivity of EC cells to carboplatin/paclitaxel by sponging miR-27b in EC.
‘Good farmers’ and ‘real vets’: social identities, behaviour change and the future of bovine tuberculosis eradication
Authors: Enticott Gareth 
Published: July 2023

AbstractThis paper considers the role of social research and human behaviour in attempts to eradicate bTB. Future attempts to eradicate bTB are likely to involve an increasing range of sophisticated technologies. However, the acceptance and use of these technologies is likely to depend on a range of behavioural incentives. The use of appropriate behavioural nudges may facilitate bTB eradication, but the paper contends that of more value are socio-cultural approaches to understanding behaviour. Specifically, the concepts of the ‘good farmer’ and ‘real vets’ are discussed to show how bTB eradication is dependent on social identities. In conclusion, the paper outlines four key roles for social research in assisting with future bTB eradication policies.
Arthroscopic Bankart repair versus conservative treatment for first-time traumatic anterior shoulder dislocation: a systematic review and meta-analysis
Authors: Hu Bin , Hong Jianqiao , Zhu Hanxiao , Yan Shigui , Wu Haobo 
Published: July 2023

AbstractBackgroundShoulder is vulnerable to dislocation owing to its anatomical structure and the increasing popularity of contact sports in young population. The management of first-time anterior shoulder dislocation in this group is still controversial and the prognosis are varied. This review aimed to compare the results of arthroscopic Bankart repair and conservative management for first-time traumatic anterior shoulder dislocation in young active patients.MethodsDatabases were searched till November 2021, and comparative studies between arthroscopic Bankart repair and conservative management for first-time traumatic anterior shoulder dislocation in young population were selected. Methodological quality of the studies was assessed according to the Cochrane Back Review Group 12-item scale. Outcome measures included recurrence of instability, return to play, subsequent instability surgery, and shoulder functional scores.ResultsThe search returned 12 eligible trials with 786 participants. All the trials were of prospective design. After arthroscopic Bankart repair, patients experienced significantly less re-dislocation (7.5% vs. 53.0%,p < 0.00001,I2 = 0%), subluxation (3.1% vs. 24.2%,p < 0.0001,I2 = 0%), positive apprehension test (7.3% vs. 25.8%,p = 0.002,I2 = 11%), and subsequent surgical treatment for instability (5.6% vs. 37.8%,p < 0.00001,I2 = 0%) when compared with those underwent conservative management. And more patients returned to play (83.5% vs. 66.0%,p = 0.03,I2 = 81%) after arthroscopic Bankart repair. Outcomes regarding the functional scores did not reach a significant difference between the two cohorts.ConclusionsArthroscopic Bankart repair showed superiority over conservative management in terms of recurrence, return to play, and subsequent instability surgery during the follow-up in young active patients that encountered first episode of dislocation. As long-term prognosis is comparable, an immediate surgical stabilization might not be suitable for everyone.
TGF-β promotes the proliferation and osteogenic differentiation of dental pulp stem cells a systematic review and meta-analysis
Authors: Gao Pengfei , Liu Chanjuan , Dong Hui , Li Qi , Chen Yunfang 
Published: July 2023

Abstract
Background
Dental pulp stem cells (DPSCs) are adult stem cells with multi-directional differentiation potential derived from ectoderm. Vitro experiments have shown that adding cytokines can help DPSCs to be transformed from multipotent stem cells to osteoblasts. TGF-β has been proved to have an effect on the proliferation and mineralization of bone tissue, but its effect on the osteogenesis and proliferation of dental pulp stem cells is still uncertain. We aim to determine the effect of TGF-β on the osteogenesis and proliferation of dental pulp stem cells.

Methods
We have identified studies from the Cochrane Central Register of Controlled Trials, PubMed, Embase, and China national knowledge infrastructure (CNKI) for studies interested in TGF-β and proliferation and differentiation of dental pulp stem cells in the following indicators: A490 (an index for evaluating cell proliferation), bone sialoprotein (BSP), Col plasmid-1 (Col-1), osteocalcin (OCN), runt-related transcription factor 2 (Runx-2); and the number of mineralized nodules. Any language restrictions were rejected. Furthermore, we drew a forest plot for each outcome. We conducted a sensitivity analysis, data analysis, heterogeneity, and publication bias test. We evaluate the quality of each study under the guidance of Cochrane's tool for quality assessment.

Results
The pooled data showed that TGF-β could promote the proliferation and ossification of dental pulp stem cells. All the included results support this conclusion except for the number of mineralized nodules: TGF-β increases the A490 index (SMD 3.11, 95% CI [0.54–5.69]), promotes the production of BSP (SMD 3.11, 95% CI [0.81–6.77]), promotes the expression of Col-1 (SMD 4.71, 95% CI [1.25–8.16]) and Runx-2 (SMD 3.37, 95% CI [− 0.63 to 7.36]), increases the content of OCN (SMD 4.32, 95% CI [1.20–7.44]) in dental pulp, and has no significant effect on the number of mineralized nodules (SMD 3.87, 95% CI [− 1.76 to 9.51]) in dental pulp stem cells.

Conclusions
TGF-β promotes the proliferation and osteogenesis of dental pulp stem cells.
The long-term gut bacterial signature of a wild primate is associated with a timing effect of pre- and postnatal maternal glucocorticoid levels
Authors: Anzà Simone , Schneider Dominik , Daniel Rolf , Heistermann Michael , Sangmaneedet Somboon , Ostner Julia , Schülke Oliver 
Published: July 2023

AbstractBackgroundDuring development, elevated levels of maternal glucocorticoids (GCs) can have detrimental effects on offspring morphology, cognition, and behavior as well as physiology and metabolism. Depending on the timing of exposure, such effects may vary in strength or even reverse in direction, may alleviate with age, or may concern more stable and long-term programming of phenotypic traits. Maternal effects on gut bacterial diversity, composition, and function, and the persistence of such effects into adulthood of long-lived model species in the natural habitats remain underexplored.ResultsIn a cross-sectional sample of infant, juvenile, and adult Assamese macaques, the timing of exposure to elevated maternal GCs during ontogeny was associated with the gut bacterial community of the offspring. Specifically, naturally varying maternal GC levels during early but not late gestation or lactation were associated with reduced bacterial richness. The overall effect of maternal GCs during early gestation on the gut bacterial composition and function exacerbated with offspring age and was 10 times stronger than the effect associated with exposure during late prenatal or postnatal periods. Instead, variation in maternal GCs during the late prenatal or postnatal period had less pronounced or less stable statistical effects and therefore a weaker effect on the entire bacterial community composition, particularly in adult individuals. Finally, higher early prenatal GCs were associated with an increase in the relative abundance of several potential pro-inflammatory bacteria and a decrease in the abundance ofBifidobacteriumand other anti-inflammatory taxa, an effect that exacerbated with age.ConclusionsIn primates, the gut microbiota can be shaped by developmental effects with strong timing effects on plasticity and potentially detrimental consequences for adult health. Together with results on other macaque species, this study suggests potential detrimental developmental effects similar to rapid inflammaging, suggesting that prenatal exposure to high maternal GC concentrations is a common cause underlying both phenomena. Our findings await confirmation by metagenomic functional and causal analyses and by longitudinal studies of long-lived, ecologically flexible primates in their natural habitat, including developmental effects that originate before birth.
Multispecies fish tracking across newly created shallow and deep habitats in a forward-restored lake
Authors: van Leeuwen Casper H. A. , de Leeuw Joep J. , van Keeken Olvin A. , Volwater Joey J. J. , Seljee Ferdi , van Aalderen Roland , van Emmerik Willie A. M. , Bakker Elisabeth S. 
Published: July 2023

Abstract
Background
Freshwater fish communities typically thrive in heterogenous ecosystems that offer various abiotic conditions. However, human impact increasingly leads to loss of this natural heterogeneity and its associated rich fish communities. To reverse this trend, we need guidelines on how to effectively restore or recreate habitats for multiple fish species. Lake Markermeer in the Netherlands is a human-created 70,000-ha lake with a uniform 4 m-water depth, steep shorelines, high wind-induced turbidity, and a declining fish community. In 2016, a forward-looking restoration project newly created a 1000-ha five-island archipelago in this degrading lake, which offered new sheltered shallow waters and deep sand excavations to the fish community.

Methods
In 2020, we assessed how omnivorous and piscivorous fish species used these new habitats by tracking 78 adult fish of five key species across local and lake-scales. We monitored spring arrival of adult fish and assessed local macro-invertebrate and young-of-the-year fish densities.

Results
Adult omnivorous Cyprinidae and piscivorous Percidae arrived at the archipelago in early spring, corresponding with expected spawning movements. During the productive summer season, 12 species of young-of-the-year fish appeared along the sheltered shorelines, with particularly high densities of common roach (Rutilus rutilus) and European perch (Perca fluviatilis). This suggests the sheltered, shallow, vegetated waters formed new suitable spawning and recruitment habitat for the fish community. Despite highest food densities for adult fish in the shallowest habitats (< 2-m), adult fish preferred minimally 2-m deep water. After spawning most Cyprinidae left the archipelago and moved long distances through the lake system, while most Percidae remained resident. This may be related to (1) high densities of young-of-the-year fish as food for piscivores, (2) medium food densities for omnivores compared to elsewhere in the lake-system, or (3) the attractiveness of 30-m deep sand excavations that were newly created and frequently used by one-third of all tracked fish.

Conclusions
New littoral zones and a deep sand excavation constructed in a uniform shallow lake that lacked these habitat types attracted omnivorous and piscivorous fish species within four years. Both feeding guilds used the littoral zones for reproduction and nursery, and notably piscivorous fish became residents year-round.
Mitochondrial DNA mutations in Medulloblastoma
Authors: Funke Viktoria L. E. , Sandmann Sarah , Melcher Viktoria , Seggewiss Jochen , Horvath Judit , Jäger Natalie , Kool Marcel , Jones David T. W. , Pfister Stefan M. , Milde Till , Rutkowski Stefan , Mynarek Martin , Varghese Julian , Sträter Ronald , Rust Stephan , Seelhöfer Anja , Reunert Janine , Fiedler Barbara , Schüller Ulrich , Marquardt Thorsten , Kerl Kornelius 
Published: July 2023

AbstractTo date, several studies on genomic events underlying medulloblastoma (MB) biology have expanded our understanding of this tumour entity and led to its division into four groups—WNT, SHH, group 3 (G3) and group 4 (G4). However, there is little information about the relevance of pathogenic mitochondrial DNA (mtDNA) mutations and their consequences across these. In this report, we describe the case of a female patient with MB and a mitochondriopathy, followed by a study of mtDNA variants in MB groups. After being diagnosed with G4 MB, the index patient was treated in line with the HIT 2000 protocol with no indications of relapse after five years. Long-term side effects of treatment were complemented by additional neurological symptoms and elevated lactate levels ten years later, resulting in suspected mitochondrial disease. This was confirmed by identifying a mutation in the MT-TS1 gene which appeared homoplasmic in patient tissue and heteroplasmic in the patient’s mother. Motivated by this case, we explored mtDNA mutations across 444 patients from ICGC and HIT cohorts. While there was no statistically significant enrichment of mutations in one MB group, both cohorts encompassed a small group of patients harbouring potentially deleterious mtDNA variants. The case presented here highlights the possible similarities between sequelae caused by MB treatment and neurological symptoms of mitochondrial dysfunction, which may apply to patients across all MB groups. In the context of the current advances in characterising and interpreting mtDNA aberrations, recognising affected patients could enhance our future knowledge regarding the mutations’ impact on carcinogenesis and cancer treatment.
The potential of fibre optic UV-Vis-NIR spectroscopy to distinguish vital and devitalised forms of microbial contamination of paper substrates
Authors: Paračková Patrícia , Čeppan Michal , Kaliňáková Barbora , Reháková Milena , Gál Lukáš 
Published: July 2023

Abstract
Microorganisms, especially microscopic filamentous fungi, represent one of the most significant factors influencing the degradation of cultural heritage cellulosic objects. Early detection, identification and characterisation of microbial communities can help select a suitable conservation intervention and, in addition, allow possible control of its effectiveness. A combination of spectral methods and statistical data processing appears to be a suitable alternative to conventional methods for monitoring microbial contamination. The main goal of the presented research was the investigation of the spectral properties of vital and devitalised filamentous fungi Alternaria alternata, Aspergillus niger, Cladosporium herbarum, Penicillium chrysogenum, and Trichoderma atroviride, inoculated on a paper substrate Whatman in two phases—dormant (conidia) and active (mycelium). The combination of the UV-Vis-NIR and NIR Fibre Optics Reflection Spectroscopy (FORS) with the Principal Component Analysis (PCA) was set to determine whether the spectra of vital and devitalised forms of studied samples differ. The obtained results prove differences between the spectra of vital and devitalised forms of filamentous fungi, at least in one studied spectral region. Therefore, UV-Vis-NIR and NIR FORS combined with PCA seems to be a promising tool for monitoring paper-based objects' microbial contamination.
The significance of sample preparation of historical and contemporary poly(vinyl chloride) objects to investigate the distribution and changes of molar mass by SEC-MALS-dRI
Authors: Rijavec Tjaša , Pawcenis Dominika , Kruczała Krzysztof , Strlič Matija , Kralj Cigić Irena 
Published: July 2023

Abstract
The distribution of molar mass of historical and contemporary poly(vinyl chloride) objects was determined using size exclusion chromatography with multi-angle light scattering detection. The weight average molar mass was determined for a collection of 57 samples, ranging from 75 to 186 kg/mol with a median of 102 kg/mol. Rigid PVC objects were found to have a significantly lower weight average molar mass (Mw = 81 kg/mol) than plasticized ones (Mw = 102 kg/mol). Thin objects also exhibited significantly lower Mw than bulky objects. A substantial presence of aggregates was noted and characterized as an ‘aggregate ratio’ for the entire collection. Heating a solution of poly(vinyl chloride) in THF at 55 °C for 5 h proved successful in the dissociation of the aggregates and allowed for an accurate determination of molar mass. The uncertainty of the Mw determination was statistically evaluated and used to study the effects of accelerated degradation on Mw. No statistically significant changes in Mw were observed in samples artificially aged at 50 °C to 80 °C for up to 15 weeks, indicating that Mw does not decrease during degradation of a PVC heritage object and that accelerated degradation does not lead to polymer crosslinking.
Feasibility, clinical efficacy, and well-being outcomes of an online singing intervention for postnatal depression in the UK: SHAPER-PNDO, a single-arm clinical trial
Authors: Bind Rebecca H. , Sawyer Kristi , Hazelgrove Katie , Rebecchini Lavinia , Miller Celeste , Ahmed Subeyda , Dazzan Paola , Sevdalis Nick , Bakolis Ioannis , Davis Rachel , Lopez Maria Baldellou , Woods Anthony , Crane Nikki , Manoharan Manonmani , Burton Alexandra , Dye Hannah , Osborn Tim , Greenwood Lorna , Perkins Rosie , Fancourt Daisy , Pariante Carmine M. , Estevao Carolina 
Published: July 2023

Abstract
Background
Postnatal depression (PND) affects over 12% of mothers, with numbers rising during COVID-19. Singing groups can support mothers with PND; however, online delivery has never been evaluated. SHAPER-PNDO, a single-arm clinical trial, evaluated the feasibility, clinical efficacy, and well-being outcomes of a 6-week online version of Breathe Melodies for Mums (M4M) singing intervention developed for mothers with PND during COVID-19 lockdowns.

Methods
The primary objective of this study was to assess the feasibility of a group online singing intervention for new mothers with postnatal depression. This was ascertained through recruitment rates, study retention rates, attendance rates to the singing sessions, and study completion rates. The secondary objective of the study was to assess the clinical efficacy and well-being outcomes of the singing intervention. Specifically, we measured change in Edinburgh Postnatal Depression Scale (EPDS), State-Trait Anxiety Inventory (STAI), Perceived Stress Scale (PSS), and Office for National Statistics Wellbeing Scale (ONS) scores from baseline to end-of-intervention (week 6); follow-up assessments were completed at weeks 3, 16, and 32. Mothers were eligible if they scored ≥10 on the baseline EPDS.

Results
Eighty-seven percent of the 37 recruited mothers completed the study, attending, on average, 5 of the 6 group singing sessions. With regard to secondary outcomes, at end-of-treatment, mothers experienced significant reductions in depression (EPDS, 16.6 ± 3.7 to 11.2 ± 5.3, 95% CI [0.79,1.65]), anxiety (STAI-S, 48.4 ± 27.1 to 41.7 ± 26.8, 95% CI [4.96, 17.65]) and stress (PSS, 29.0 ± 5.7 to 19.7 ± 5.3, 95% CI [1.33, 7.07]); and, furthermore, significant improvements in life satisfaction (ONS, 50.5 ± 23.0 to 72.8 ± 11.7, 95% CI [− 39.86, − 4.64]) and feelings of worthwhileness (ONS, 51.7 ± 30.4 to 78.6 ± 15.1, 95% CI [− 52.79, − 0.85]). Reduction on the EPDS correlated with a reduction on the BDI and the STAI-S and maternal childhood maltreatment was predictive of a smaller treatment response.

Conclusions
M4M online was feasible to mothers who partook in the programme. Furthermore, M4M online supports the mental health and well-being of new mothers experiencing PND, especially when barriers to in-person treatment are present.

Trial registration
ClinicalTrials.gov NCT04857593. Registered 22 April 2021, retrospectively registered;
Efficacy of pure beta tricalcium phosphate graft in dentoalveolar surgery: a retrospective evaluation based on serial radiographic images
Authors: Choi Young-Jin , Chang Hoon-Je , Kim Min Jae , Lee Jee-Ho , Lee Bu-Kyu 
Published: July 2023

Abstract
Background
The use of beta-tricalcium phosphate (beta-TCP) in dental surgery is limited owing to its rapid absorption compared to mixed formulations of hydroxyapatite. However, newly developed pure beta-TCP crystals have demonstrated slow absorption; hence, they last longer within the defect and act as a scaffold until new bone formation. The oral environment is unique and can prove unfavorable for bone grafts due to the high infection rate in the oral cavity and the fragile condition of the oral mucosa. The aim of this study was to evaluate the feasibility of using pure beta-TCP bone grafts in various dental treatments.

Methods
Panoramic X-ray images of 25 patients who underwent bone grafting during dental surgery were analyzed. A specially treated pure beta-TCP crystal, Neo Bone® (Neo Bone®, SN Biologics Co., Ltd, Seoul, Korea), was used in this study. The bone density at the graft site was compared with that of the surrounding bone using the ImageJ software (Wayne Rasband, NIH USA).

Results
Six months after surgery, the bone graft density was similar to that of the surrounding bone in 20 patients and increased in 5 patients. No adverse effects, such as infection, dehiscence, or graft failure, were observed.

Conclusion
The newly developed pure beta-TCP crystal was slowly absorbed and served as support until new bone formation at the defect site, thus demonstrating its potential for use in various oral conditions requiring bone grafting.
Iatrogenic uvular injury after endotracheal intubation: recommendations for clinical practice
Authors: Zameer Sheharyar , Inam Syed Muhammad , Faisal Mahvish , Khan Huma Ahmed , Fatima Anmol , Anwar Umer 
Published: July 2023

Abstract
Background
Uvular injury is a rare complication of endotracheal intubation. It presents as a triad of sore throat, foreign body sensation in pharynx, and halitosis.

Case presentation
A 26-year-old male who underwent endotracheal intubation for general anesthesia developed sore throat and foreign body sensation after the procedure. The patient was diagnosed as a case of iatrogenic uvular necrosis (grade III uvular injury) leading to auto-amputation (grade IV uvular injury) attributed to compression of the uvula due to the endotracheal tube placement. The patient was counseled regarding the etiology of the disease and was treated conservatively.

Conclusions
Uvular injury is a rare complication that can easily be prevented by corrections in technique and experience. This report emphasizes the establishment of principles of diagnosis, prevention, and treatment of uvular injury.