bluuebunny/crossref_metadata_2025_split huggingface dataset:
Total Force Fitness Standardization: Leveraging Policy and Metrics to Inform and Accelerate Implementation
Authors: Stepanenko Bryan A , Givens Melissa 
Published: September 2023

ABSTRACT
Total Force Fitness (TFF) metrics inform leaders at every level as they develop and evaluate policies, practices, and programs that enable soldiers, airmen, sailors, marines, guardians, and operators to achieve human performance optimization in all eight domains as defined by the TFF framework. The Standardization Workgroup was a facilitated discussion breakout session among a group of uniquely informed and engaged attendees who were invited to be part of the TFF summit that was hosted in Bethesda, Maryland, on September 14 and 15, 2021. The Standardization Workgroup explored the 2018 TFF shift in perspective to consider human performance optimization in the context of a military unit’s fitness for missions and identified several challenges, opportunities, and action items for improving the implementation and sustainment of TFF throughout the services. Key opportunities and recommendations synthesized from the workgroup discussion include the following: Creating true ownership of TFF by an entity with authority and engagement across the services; creating divisions of labor and assigning champion organizations with distinct areas of responsibility related to metric development, data accessibility, line-leader decision support, and data-driven policy and program review; leveraging and expanding the use of data repositories, secure data clouds, and artificial intelligence platforms to support a robust and integrated learning health and performance system; and prioritizing the operational line-leader stakeholder engagement at every stage of metric development and refinement as they are ultimately responsible for and have direct influence over the fitness of the individual service member and readiness to deploy of the military unit.
What drives the variance of galaxy spectra?
Authors: Sharbaf Zahra , Ferreras Ignacio , Lahav Ofer 
Published: September 2023

ABSTRACT
We present a study aimed at understanding the physical phenomena underlying the formation and evolution of galaxies following a data-driven analysis of spectroscopic data based on the variance in a carefully selected sample. We apply principal component analysis (PCA) independently to three subsets of continuum-subtracted optical spectra, segregated into their nebular emission activity as quiescent, star-forming, and active galactic nuclei (AGNs). We emphasize that the variance of the input data in this work only relates to the absorption lines in the photospheres of the stellar populations. The sample is taken from the Sloan Digital Sky Survey (SDSS) in the stellar velocity dispersion range 100–150 km s−1, to minimize the ‘blurring’ effect of the stellar motion. We restrict the analysis to the first three principal components (PCs) and find that PCA segregates the three types with the highest variance mapping SSP-equivalent age, along with an inextricable degeneracy with metallicity, even when all three PCs are included. Spectral fitting shows that stellar age dominates PC1, whereas PC2 and PC3 have a mixed dependence of age and metallicity. The trends support – independently of any model fitting – the hypothesis of an evolutionary sequence from star formation to AGN to quiescence. As a further test of the consistency of the analysis, we apply the same methodology in different spectral windows, finding similar trends, but the variance is maximal in the blue wavelength range, roughly around the 4000 Å break.
ZEB1 promotes non-homologous end joining double-strand break repair
Authors: Genetta Thomas L , Hurwitz Joshua C , Clark Evan A , Herold Benjamin T , Khalil Shadi , Abbas Tarek , Larner James M 
Published: September 2023

Abstract
Repair of DSB induced by IR is primarily carried out by Non-Homologous End Joining (NHEJ), a pathway in which 53BP1 plays a key role. We have discovered that the EMT-inducing transcriptional repressor ZEB1 (i) interacts with 53BP1 and that this interaction occurs rapidly and is significantly amplified following exposure of cells to IR; (ii) is required for the localization of 53BP1 to a subset of double-stranded breaks, and for physiological DSB repair; (iii) co-localizes with 53BP1 at IR-induced foci (IRIF); (iv) promotes NHEJ and inhibits Homologous Recombination (HR); (v) depletion increases resection at DSBs and (vi) confers PARP inhibitor (PARPi) sensitivity on BRCA1-deficient cells. Lastly, ZEB1’s effects on repair pathway choice, resection, and PARPi sensitivity all rely on its homeodomain. In contrast to the well-characterized therapeutic resistance of high ZEB1-expressing cancer cells, the novel ZEB1-53BP1-shieldin resection axis described here exposes a therapeutic vulnerability: ZEB1 levels in BRCA1-deficient tumors may serve as a predictive biomarker of response to PARPis.
Structural basis of transcriptional activation by the OmpR/PhoB-family response regulator PmrA
Authors: Lou Yuan-Chao , Huang Hsuan-Yu , Yeh Hsin-Hong , Chiang Wei-Hung , Chen Chinpan , Wu Kuen-Phon 
Published: September 2023

Abstract
PmrA, an OmpR/PhoB-family response regulator, triggers gene transcription responsible for polymyxin resistance in bacteria by recognizing promoters where the canonical-35 element is replaced by the pmra-box, representing the PmrA recognition sequence. Here, we report a cryo-electron microscopy (cryo-EM) structure of a bacterial PmrA-dependent transcription activation complex (TAC) containing a PmrA dimer, an RNA polymerase σ70 holoenzyme (RNAPH) and the pbgP promoter DNA. Our structure reveals that the RNAPH mainly contacts the PmrA C-terminal DNA-binding domain (DBD) via electrostatic interactions and reorients the DBD three base pairs upstream of the pmra-box, resulting in a dynamic TAC conformation. In vivo assays show that the substitution of the DNA-recognition residue eliminated its transcriptional activity, while variants with altered RNAPH-interacting residues resulted in enhanced transcriptional activity. Our findings suggest that both PmrA recognition-induced DNA distortion and PmrA promoter escape play crucial roles in its transcriptional activation.
Mental health and vitality predict spinal pain in healthcare workers
Authors: Espin A , Núñez-Cortés R , Irazusta J , Rodriguez-Larrad A , Torres-Unda J , Vinstrup J , Jakobsen M D , Andersen L L 
Published: September 2023

Abstract

Background
Despite extensive investigation of ergonomic risk factors for spinal pain in healthcare workers, limited knowledge of psychological risk factors exists.

Aims
To assess the prospective association of mental health and vitality with development of spinal pain in healthcare workers.

Methods
A prospective cohort study was carried out involving 1950 healthcare workers from 19 hospitals in Denmark. Assessments were done at baseline and at 1-year follow-up. Mental health and vitality were measured using the Short Form-36 Health Survey, while spinal pain intensity was measured using a 0–10 scale in the low-back, upper-back and neck, respectively. Cumulative logistic regressions adjusted for several confounding factors were applied, reporting risk estimates as odds ratios (ORs) and 95% confidence intervals (CIs).

Results
Using good mental health as reference, moderate (but not poor) mental health at baseline was associated with increased pain intensity in the low-back (OR: 1.41 [95% CI: 1.21–1.77]), upper-back (OR: 1.63 [95% CI: 1.31–2.02]) and neck (OR: 1.31 [95% CI: 1.07–1.61]) at 1-year follow-up. Likewise, using high vitality as reference, both moderate and low vitality at baseline were associated with increased pain intensity in the low-back (OR: 1.54 [95% CI: 1.22–1.94] and OR: 2.34 [95% CI: 1.75–3.12], respectively), upper-back (OR: 1.72 [95% CI: 1.34–2.23] and OR: 2.46 [95% CI: 1.86–3.25], respectively) and neck (OR: 1.66 [95% CI: 1.34–2.06] and OR: 2.06 [95% CI: 1.61–2.63], respectively) at 1-year follow-up.

Conclusions
Compared to healthcare workers with good mental health and high vitality, those with moderate mental health and low/moderate vitality, respectively, were more likely to increase spinal pain intensity at 1-year follow-up. These components should also be considered in the prevention of spinal pain in healthcare workers.
Discovery of a molecular cloud possibly associated with the youngest Galactic SNR G1.9+0.3
Authors: Enokiya Rei , Sano Hidetoshi , Filipović Miroslav D , Alsaberi Rami Z E , Inoue Tsuyoshi , Oka Tomoharu 
Published: September 2023

Abstract
The youngest known Galactic supernova remnant (SNR) G1.9+0.3 has high-velocity supernova shock beyond 10000 km s−1, and it is considered to be one of the major candidates of a PeVatron. Despite these outstanding properties, the surrounding interstellar matter of this object is poorly understood. We investigated the interstellar gas toward G1.9+0.3 using the 12CO(J = 3−2) data with the angular resolution of 15″ obtained by the CHIMPS2 survey by the James Clerk Maxwell Telescope, and discovered three individual clouds at −1, 7, and 45 km s−1. From its morphological and velocity structures, the −1 km s−1 cloud, having the largest velocity width &gt;20 km s−1 and located at the distance of the Galactic Center, is possibly associated with the SNR. The associated cloud shows a cavity structure both in space and velocity and coincides well with the SNR. We found that the associated cloud has higher column densities toward three bright, radio synchrotron-emitted rims where the radial expansion velocity of the supernova shock is decelerated, and the cloud is faint in the other parts of the SNR. This is the first direct evidence indicating that the highly anisotropic expansion of G1.9+0.3 observed by previous studies results from the deceleration by the interaction between the supernova shock and surrounding dense interstellar medium.
Spatiotemporal distribution of reactive oxygen species production, delivery, and use in Arabidopsis root hairs
Authors: Kuběnová Lenka , Haberland Jan , Dvořák Petr , Šamaj Jozef , Ovečka Miroslav 
Published: September 2023

Abstract
Fluorescent selective probes for reactive oxygen species (ROS) detection in living cells are versatile tools for the documentation of ROS production in plant developmental or stress reactions. We employed high-resolution live-cell imaging and semiquantitative analysis of Arabidopsis (Arabidopsis thaliana) stained with CM-H2DCFDA, CellROX Deep Red, and Amplex Red for functional characterization of the spatiotemporal mode of ROS production, delivery, and utilization during root hair formation. Cell viability marker fluorescein diacetate served as a positive control for dye loading and undisturbed root hair tip growth after staining. Using a colocalization analysis with subcellular molecular markers and two root hair mutants with similar phenotypes of nonelongating root hairs, but with contrasting reasons for this impairment, we found that: (i) CM-H2DCFDA is a sensitive probe for ROS generation in the cytoplasm, (ii) CellROX Deep Red labels ROS in mitochondria, (iii) Amplex Red labels apoplastic ROS and mitochondria and shows high selectivity to root hairs, (iv) the root hair defective 2-1 (rhd2-1) mutant with nonfunctional NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN C/ROOT HAIR-DEFECTIVE 2 (AtRBOHC/RHD2) has a low level of CM-H2DCFDA-reactive ROS in cytoplasm and lacks Amplex Red-reactive ROS in apoplast, and (v) the ACTIN2-deficient deformed root hairs1-3 (der1-3) mutant is not altered in these aspects. The sensitivity of CellROX Deep Red was documented by discrimination between larger ROS-containing mitochondria and small, yet ROS-free premature mitochondria in the growing tip of root hairs. We characterized spatial changes in ROS production and compartmentalization induced by external ROS modulators, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and ionophore valinomycin. This dynamic and high-resolution study of ROS production and utilization opens opportunities for precise speciation of particular ROS involved in root hair formation.
The school counselor’s role in addressing ACEs: an intervention strategy
Authors: Prasetya Yovian Yustiko , Setiawan Dedy , Si Suwarjo M , Aditama Mint Husen Raya , Selfiardy Syafrida 
Published: September 2023

Abstract
Children growing up with unemployed parents may be at higher risk of adverse childhood experiences (ACEs). The role of school counselors in identifying and providing services to children at risk of ACEs is important. In addition, the intervention strategy is expected to bridge the role between schools and the government in overcoming the risks of ACEs in the community.
Abstract 4 Excellent Event-Free-Survival and Immunoglobulin Independence for Patients with Severe Combined Immunodeficiency Transplanted with Umbilical Cord Blood Stem Cells
Authors: Ubico Erin Morales , MacMath Derek , Omer Bilal , Doherty Erin , Llaurador Gabriela , Sasa Ghadir , Salem Baheyeldin , Khaled Yassine , Steffin David , Craddock John , George Anil , Brenner Malcolm , Heslop Helen , Bhar Saleh , Forbes Lisa , Nicholas Sarah , Noroski Lenora , Rider Nicholas , Chinn Ivan , Seeborg Filiz , Chinen Javier , Davis Carla , Pena Amanda , Wan Tao , Wu Mengfen , Shaver Kristen , Krance Robert , Martinez Caridad 
Published: September 2023

Abstract

Introduction
An HLA matched related donor (MRD) is the preferred donor source for performing a hematopoietic stem cell transplant (HSCT) in patients with severe combined immunodeficiency (SCID) (Heimall 2017). However, many patients lack an MRD and thus an alternative donor, must be used. In these situations, an umbilical cord blood (UCB) represents a promising stem cell source (Gragert 2014, Martinez 2022).

Objectives
The primary outcome of interest was one-year event free survival (EFS) and the secondary outcome was time to immunoglobulin therapy independence based on donor source.

Methods
All patients diagnosed with SCID who underwent HSCT at Texas Children’s Hospital from 2003 to 2021 were included. EFS was defined as survival 365 days post-transplant without additional transplant, stem cell “top-off”, or donor lymphocyte infusion. The date of last immunoglobulin infusion was recorded to track time to immunoglobulin therapy independence.

Results
A total of 67 patients were included in the cohort. One-year EFS was 74.25% for all patients using the Kaplan-Meier method. When stratified by donor/stem cell source, EFS was 88.94% for UCB, 72.92% for MRD, 60.00% for URD, and 41.67% for haplo. There was statistical significance in EFS between URD UCB and the haploidentical group (p = 0.0006).
The median time to IVIG independence was 8.6 months for all patients, 7.3 months for UCB, 23.4 months for URD, 26.6 months for haplo, and 32.5 months for MRD. When stratified by donor/stem cell source, cumulative incidence analysis showed UCB patients achieved Immunoglobulin independence earlier when compared to all other groups (UCB vs haplo p = 0.0335, UCB vs MRD p = 0.0012, UCB vs URD p = 0.0488).

Discussion
This single-center cohort of 67 patients demonstrates excellent survival for SCID patients undergoing HSCT, confirming prior studies (Pai 2014, Heimall 2017). EFS was highest in patients treated with an UCBT. In our analysis of immune reconstitution, we found that SCID patients transplanted with UCB, had a significantly shorter time to IVIG independence compared to other stem cell donors/sources. Given the high survival of UCB patients and this robust immune reconstitution, UCB may represent the best alternative stem cell source for SCID patients without a MRD option.
Abstract 6 Combined Umbilical Cord Mesenchymal Stromal/Stem Cell Application For Primary Torsion Dystonia Treatment (Case Report)
Authors: Petriv Taras , Vorodi Milan , Tsymbaliyk Yaroslav , Tsymbaliuk Yulia , Luzan Boris , Tsymbaliuk Vitaliy 
Published: September 2023

Abstract

Introduction
Primary torsion dystonia (PTD) is a neurological disorder characterized by sustained or intermittent muscle contractions causing abnormal movements and/or postures with severe disability of young patients. Traditional therapies for PTD include oral anti-parkinsonism drugs, botulinum neurotoxin, pallidotomy and deep brain stimulation. Stem cell therapy is another promising treatment option, due to its neuroregenerative properties.

Objectives
The objective of the study was to investigate the effect of combined (intrathecal and intravenous) administration of umbilical cord derive stromal/stem cells (UC-MCS) on PTD reduction.

Methods
Patient M, born on 25.06.2004, fell ill gradually, 8 years ago developed signs of muscle dystonia, especially in right hand. Genetic tests were carried out, which revealed mutations in the DYT-1 gene. The diagnosis of primary torsion dystonia was established. Conservative treatment was not effective. The patient was offered treatment in the frame of research scientific program "To investigate the effectiveness of regenerative cell technologies in the neurosurgical treatment of patients with demyelinating diseases of the CNS and cerebral palsy. № 0119U000112" UC-MSCs for this study were isolated from healthy human umbilical cord and prepared due to the minimal criteria established by the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy. 3 injections of UC-MSC were performed with time interval of 6 month (intravenously and intrathecally in a dose of 6 × 107at each injection). Intrathecal administration was performed according to the typical method of performing lumbar puncture in compliance with the rules of asepsis and antiseptics in the operation room using local anesthesia.

Results
Prior to stem cells injection, the patient's Unified Dystonia Rating Scale (UDRS) dystonia movement score was 15, which progressively decreased after procedure to 13, 10, 7 and 4 after 6, 12, 18 and 24 months respectively. There was a significant improvement in the patient's quality of life.

Discussion
UC-MSC significantly reduce dystonia in patient. This result is probably achieved due to the neuroregenerative and neurotrophic effects of UC-MSCs. Study results suggest the need to provide larger multicenter randomized studies with more sophisticated methods of investigations.
Abstract 11 Monitoring Cord Blood Inventory Reliability by Key Quality Indicators At and After Release
Authors: Frenet Emeline Masson , Sung Dorothy , Watanabe Chiseko , Chowtee Bryana , Bentsen Helen , Liu WenJun , Tabar Sara , Romeo Cynthia , Lu Ling , Shi Patricia , Jimenez Alexandra 
Published: September 2023

Abstract

Introduction
As per FACT standards, Cord Blood Banks (CBB) have to verify that they provide a safe and effective product for transplant. Validated processing methods and storage conditions are the first steps towards this goal, but what are the final steps to monitor the quality of the cord blood units (CBU) released for transplant?

Objectives
We present here a retrospective analysis of our Program’s final verification process to meet this FACT requirement.

Methods
CBU shipped between January 2018 and December 2022 were reviewed for out of specification events. The following Quality Control (QC) parameters were measured on attached segments before shipment: total nucleated cells (TNC), viable CD34 cells (vCD34), and colony forming units (CFU). Confirmed date of infusion and engraftment data were obtained from CIBMTR.

Results
A total of 619 CBU were shipped during the study period. 550 had a segment analyzed at the time of shipment. Average post-thaw CD34 viability on the segments was 93.5% (Table 1). Average recoveries for TNC, viable CD34 and CFU were 83.3%, 64.90% and 60.86%, respectively (Table 1). 6 (1%) CBUs were found to have CD34 viability lower than FACT requirement (≥70%) (Table 1). Investigations were performed for each CBU, concluding that these were technical issues with no repercussion on the final product. No segments demonstrated absence of CFU growth. 544 CBUs had an infusion date recorded by CIBMTR (Table 1). 250 of the CBU infused had engraftment data, and 242 engrafted. 8 (3%) CBU transplants resulted in non-engraftment. All had QC results within expected range (Table 1).

Discussion
Our QC results confirm the continued stability of the CBU until shipment. The small subset of CBU with out of specification results were linked to technical issues and did not affect the outcome of transplant. Outcome data is another way of assessing the quality of the CBUs, even though it may be influenced by many factors out of the CBB’s responsibility (such as diagnosis, conditioning, multiple transplants, etc…). The combined analysis of the two datasets is a good way for a CBB to ensure processes are performing as expected over time and monitor the quality of the final product.
Abstract 32 Expanded Umbilical Cord Blood CD34+ Cells Are Immunomodulatory in a Rat Model of Neonatal Brain Injury
Authors: Penny Tayla , Watt Ashalyn , Marzan Ricki , Pham Yen , Sutherland Amy , Smith Madeleine , Jenkin Graham , Miller Suzanne , McDonald Courtney 
Published: September 2023

Abstract

Introduction
Hypoxic ischemic (HI) insults during pregnancy and birth can result in neurodevelopmental disorders, including cerebral palsy. We have previously shown that multiple doses of umbilical cord blood (UCB) cells are needed to effectively reduce long-term neuropathology and behavioural outcomes. Unfortunately, for multiple doses it is likely that UCB expansion will be required to increase the number of cells available for treatment.

Objectives
In this study, we aimed to investigate the efficacy of expanded UCB derived CD34+ cells in modulating perinatal brain injury.

Methods
UCB derived CD34+ cells were expanded over a 14-day period using a previously optimised expansion protocol. HI injury was induced in postnatal day (P) 10 rats by left carotid artery ligation, followed by 90min hypoxia (8% oxygen). Pups were administered 1 million expanded UCB cells (eUCB) on P11, 13 and 20. Rats underwent extensive behavioural testing and post-mortem was performed on either P17 or P50 where tissue was collected for flow cytometry or immunohistochemistry analysis.

Results
After the 14-day expansion there was a ~800-fold increase in cell number with 70.7±2.18% CD34+ cells. Flow cytometry at P17 showed a significant increase in peripheral regulatory T-cells following eUCB administration (P=0.047). At P50, HI brain injury resulted in significant behavioural deficits and adverse neuropathologies. Multiple doses of eUCB significantly reduced microglial activation compared to HI in the cortex (P=0.02) and the hippocampus (P=0.018).

Discussion
Results from this study show for the first time that expanded UCB-derived CD34+ cells are a promising treatment option for perinatal brain injury as they have significant anti-inflammatory properties.