bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-specific effects of the sole C. elegans Daughterless/E protein homolog, HLH-2, on nervous system development
Authors: Masoudi Neda , Schnabel Ralf , Yemini Eviatar , Leyva-Díaz Eduardo , Hobert Oliver 
Published: January 2023

ABSTRACT
Are there common mechanisms of neurogenesis used throughout an entire nervous system? We explored to what extent canonical proneural class I/II bHLH complexes are responsible for neurogenesis throughout the entire Caenorhabditis elegans nervous system. Distinct, lineage-specific proneural class II bHLH factors are generally thought to operate via interaction with a common, class I bHLH subunit, encoded by Daughterless in flies, the E proteins in vertebrates and HLH-2 in C. elegans. To eliminate function of all proneuronal class I/II bHLH complexes, we therefore genetically removed maternal and zygotic hlh-2 gene activity. We observed broad effects on neurogenesis, but still detected normal neurogenesis in many distinct neuron-producing lineages of the central and peripheral nervous system. Moreover, we found that hlh-2 selectively affects some aspects of neuron differentiation while leaving others unaffected. Although our studies confirm the function of proneuronal class I/II bHLH complexes in many different lineages throughout a nervous system, we conclude that their function is not universal, but rather restricted by lineage, cell type and components of differentiation programs affected.
Expression of modified enhanced green fluorescent polyarginine protein in Saccharomyces cerevisiae INVSc1
Authors: Tandio Saputro Shania Safera , Wahyunita Khayu , Nurhasanah Astutiati , Nugraha Yudhi , Faizal Irvan , Pambudi Sabar , Pramono Andri Pramesyanti 
Published: January 2023

Background: The enhanced green fluorescent protein (EGFP) gene is a reporter gene that can be used to optimize protein isolation procedures and the functional working of a transduction protein. EGFP, with the addition of eleven arginine residues, has been engineered to functionally improve the protein transduction process, which can later be used for cell reprogramming like induced pluripotent stem cells. The addition of six histidine amino acid residues at its C-terminal is intended for the protein isolation process using the His-tag antibody. Methods: The study aimed to investigate the optimization of the EGFP polyarginine protein expression in Saccharomyces cerevisiae in sufficient quantities for the protein isolation stage. This study also analyzed EGFP expression without polyarginine to analyze the polyarginine addition effect on expression processes. Protein expression was qualitatively measured by looking at expression fluorescence and protein levels of EGFP and EGFP - PolyR proteins. Results: Bands on Western Blots with 6×His-tag monoclonal antibody (primary antibody) and Goat anti-mouse IgG HRP (secondary antibody) showed the EGFP polyarginine and EGFP proteins were expressed in Saccharomyces cerevisiae INVSc1 at relatively low levels. The lyticase incubation time modification and administration of 3-5 kDa microfilter to concentrate increased the yield of isolated protein. Conclusions: The sufficient amount of protein isolation in S. cerevisiae can be achieved by using lyticase and sonicators combination for the lysis process.
CZŁOWIEK I TOŻSAMOŚĆ NARODOWA POZA GRANICAMI KRAJU. DZIEJE ARCHIWUM POLSKIEGO HOSPICJUM I KOŚCIOŁA ŚW. STANISŁAWA W RZYMIE W PERSPEKTYWIE DALSZYCH BADAŃ
Authors: Skrabski Józef , Sumieniewski Cezary , Kochańczyk-Bonińska Karolina 
Published: January 2023

Artykuł prezentuje dotychczasowe prace inwentaryzacyjne, digitalizacyjne oraz naukowo-badawcze podjęte w Archiwum i Hospicjum św. Stanisława w Rzymie oraz zarysowuje dalsze perspektywy w zakresie ochrony, inwentaryzacji, udostępniania archiwaliów oraz możliwości badawcze związane z zasobami Archiwum, biorąc pod uwagę specyfikę zgromadzonych zbiorów i ich znaczenie dla rekonstrukcji losów poszczególnych osób, rodzin oraz historii organizacji i środowisk polonijnych rozsianych po całym świecie. To z kolei ma istotne znaczenie dla budowania tożsamości narodowej Polaków zamieszkałych poza granicami kraju.
Is vitamin D supplementation of potential benefit for community-living people with Alzheimer's disease?
Authors: Karaoglan Mehmet , Voegeli David 
Published: January 2023

Background Vitamin D is associated not only with effects on calcium and bone metabolisms but also with many chronic diseases. Low vitamin D levels in patients with Alzheimer's disease have been widely reported in the literature. Aim The purpose of this study was to critically review the potential benefit of vitamin D supplementation in individuals with Alzheimer's disease living in the community. Methods: A systematic literature search was conducted in PubMed, CINAHL, EMBASE and the Cochrane Library for papers published 2011–2018. Results Seven papers were selected, consisting of one clinical trial, five cohort studies and one systematic review. Studies showed an association only between vitamin D deficiency and lower attention in older people. None of the reviewed studies provided evidence of a positive impact of vitamin D supplementation on cognitive function in older people with Alzheimer's disease. Conclusion There was no evidence that vitamin D supplementation has a direct benefit for Alzheimer's disease. The review synthesised the existing body of knowledge and concluded that optimum levels of vitamin D (neither too low nor too high) do appear to have positive effects on patient outcomes and quality of life. It is still unclear why vitamin D intake is inadequate as people age. Further research is needed to clarify vitamin-D-related aspects of Alzheimer's disease.
Hypothalamic Revascularization and Rejuvenation
Authors: Rafael Hernando , Valadez María Teresa 
Published: January 2023

Background: Since 1990 and to date, four therapeutic methods have been used against aging. All of them, to increase the plasma growth hormone levels.
Material and Method: We choose 38 patients to analyse the postoperative rejuvenation. Previously, all of them have been received omental transplantation on the optic chiasma, the carotid bifurcation and the anterior perforated space. Patients with stroke in 14 cases, Alzheimer´s disease (AD) in 23 and Huntington´s disease (HD) in 1. The clinical data of rejuvenation were obtained by observation, videotape and photography.
Results: Neurological improvement in patients with stroke, AD and HD were better during the first weeks after surgery than in the following months or years. Besides this, between 1 to 4 months after surgery, 32 patients (84.21%) showed rejuvenation and in the rest, there were not changes. These 6 last patients presented dilation of the third ventricle.
Conclusions: These results indicate that the primary cause of aging is of ischemic origin, in the arcuate nucleus of the hypothalamus. Because, in contrast to this, its revascularization by means of omental tissue produce rejuvenation. Therefore, our surgical method improve the function of the residual arcuate nucleus and adjacent zones.
Efficiency of 0.01% Dexamethasone Solution in Comprehensive Therapy of Dry Eye Disease
Authors: Vladimir Brzheskiy , Vladimir Popov , Irina Kalinina , Natalia Kalinina 
Published: January 2023

Introduction: At present anti-inflammatory therapy of patients with dry eye disease is based mainly on glucocorticoids' instillations. In spite of the fact that dexamethasone in officinal dosage (0.1%) has a marked local anti-inflammatory effect, its wide use is limited by the presence of a destructive process in the cornea. Taking this into account, the authors developed a drug containing 0.01% dexamethasone phosphate in combinations with 6% polyvinylpyrolidone and 1.5-5.5% dextrose solution.
Objective: To study the impact of the developed medication on the inflammatory process dynamics in the tissues of the eye surface in patients with dry eye disease of various etiology.
Materials and Methods: The material of this study was based on the results of the examination and treatment of 25 volunteers (50 eyes) with corneal-conjunctival xerosis developed on the background of 7 cases of meibomian blepharitis (14 eyes), 8 cases of perimenopause (16 eyes), and 10 cases of Sjögren's syndrome (20 eyes).
All patients used the developed medication in the form of eye drops 3-4 times daily on the background of tear replacement therapy. Prior to the therapy and on day 28 of the study the following parameters were assessed, lower tear meniscus index, precorneal tear film production, stability and osmolarity, staining of eye surface epithelium with sodium fluoresce in solution, as well as bengal rose and lissamine green. The quantity of cytokines was determined in the tear fluid and blood plasma with ELISA method: interleukins-1β, 2, 4, 6, 8, 10, 17A, interleukin 1 receptor antagonist, TNF α, interferons α and γ. Besides, all the subjects were asked to fill in a questionnaire to evaluate subjective signs of the ocular surface epithelium xerosis or Ocular Surface Disease Index (OSDI).
Results: By day 28 of the study statistically valid increase of the tear meniscus index, precorneal tear film stability, main and total tear production and decrease of tear film osmolarity were observed. Besides, the staining degree of the ocular surface epithelium with bengal rose and lissamine green (van Bijsterv?ld scale) and with sodium fluoresce in (Oxford scale) decreased. Also, the positive dynamics of the objective parameters of the ocular surface epithelium is confirmed by the subjects' patients evaluation of their quality of life.
Conclusion: The results of the study performed prove the high clinical efficiency of the developed medication that has a marked local anti-inflammatory effect in the therapy of dry eye disease of various etiology.
Biochemical, Immunological, DNA and Histopathological Changes Caused by Cannabis Sativa in the Rat
Authors: Abdel-Salam Omar M.E. , Youness Eman R. , Shaffee Nermeen 
Published: January 2023

The aim of this study was to investigate the effect of repeated administration of Cannabis sativa extract on some biochemical, immunological parameters, on DNA damage and on brain and liver histology in the rat. Rats received either saline or Cannabis sativa at 5, 10 or 20 mg/kg (expressed as ?9-tetrahydrocannabinol), intraperitoneally daily for 30 days. Cholesterol, triglycerides, low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), total proteins, glucose were determined in serum. DNA fragmentation was measured in the liver. The level of DNA damage of peripheral blood mononuclear cells (PBMCs) was evaluated by alkaline single cell gel electrophoresis (comet assay). Brain and liver histopathology and caspase-3 immunohistochemistry were performed. Cannabis treatment resulted in increased cholesterol (41.2% by 20 mg/kg cannabis) and triglycerides (17.8-31.8% by 10-20 mg/kg cannabis). HDL-C decreased by 21.9-38.4% while LDL-C increased by 14.7-174.7% by 10-20 mg/kg cannabis. Total proteins were unaltered but glucose increased by 34.2-53.3% by 10-20 mg/kg of cannabis. CD4 increased by 16.6-19.5% by 10-20 mg/kg cannabis. Liver DNA fragmentation increased by 16.7% after cannabis at 20 mg/kg. The comet percentage of PBMCs was higher after 5, 10 and 20 mg/kg cannabis (7.0 ± 0.36, 9.0 ± 0.92 and 21.0 ± 0.97) than that in saline control group (5 ± 0.36). Cannabis caused dark neurons, decreased size of nuclei, cellular infiltration and increased caspase-3 immunoreactivity. In the liver, cannabis treatment was associated with fibrosis, vacuolar degeneration, cellular infiltration in the portal area, dilatation of portal vein and positive reaction to caspase-3 antibody. These effects of cannabis were dose-dependent.
Effect of Type II Diabetes Mellitus on the 8th Cranial Nerve: A Prospective Study
Authors: Viswanatha B. , Priyadarshini V. , Vijayashree M.S. , Rasika R. , Krishna Nisha 
Published: January 2023

Objective: To establish the effect of type II diabetes mellitus on the 8th cranial nerve and to analyse the association of degree of hearing loss with the factors such as duration of diabetes, age and gender of the subject and glycaemic control. Study Design: Prospective comparative type of study conducted on 100 cases and 100 age and gender matched controls. Results: Hearing loss was more prevalent and of a higher degree among the diabetics as compared to the non-diabetics. The hearing loss was significantly more among males and in those with higher post prandial blood sugar levels.
Cancer and Associated Recent Findings on Metastasis
Authors: Beeram Eswari 
Published: January 2023

Background: Cancer is one of the disease with up rised constant mutations and difficult to cure due to metastatic tumors and cell- cell communications between the tumors and the cell through stromal microenvironment and cell signaling.
Discussion: Integrin α11β1 acts as a stromal receptor and remodifies collagen through communication between the stroma and collagen related genes contributing majorly to metastasis in NSCLC. Extracellular matrix protein-1, Osteopontin and Osteonectin are the secreted proteins which are differentially expressed based on the metastatic phenotype. Thrombospondin-1 is responsible for migratory property of the wild cells by promoting intravasation and hyperplasia in many cancers like prostate cancer. Hemopexin and Ferritin light chain (FTL) are the proteins majorly expressed in cancer cell cytoplasm and stromal fibroblasts and contribute to metastasis through secretions mediated by extracellular vesicles.
Conclusion: Other proteins like TWIST, BRCA-1, BRCA-2, cell adhesion proteins, MMPs, Tetraspanin can contribute to metastasis in cancer through promoting either cell migration, Extravasation, Hyperplasia or Angiogenesis facilitating the secondary tumor formation in distant organs.
Effect of Different Etching Modes on Shear Bond Strength of Universal Adhesives to Enamel
Authors: Haider Nida , Nagpal Rajni , Singh Udai Pratap , Umar Madeha , Mehmood Nida 
Published: January 2023

Aim: To evaluate the effect of different etching modes on the bonding efficacy of two universal adhesives to enamel.
Materials and method: The study was performed in fifty five extracted human molar teeth. For shear bond strength testing, flat buccal surfaces were prepared. Teeth were randomly divided into five experimental groups according to two universal adhesives Single Bond Universal (3M ESPE, U.S.A) and Tetric N-Bond Universal (IvoclarVivadent) used in different etching modes i.e etch-and-rinse and self-etch mode and one control group of etch & rinse adhesive (Adper Single bond-2). Teeth were mounted in self-cure acrylic resin and composite restorations were placed in all the samples after application of adhesives according to the manufacturer’s instructions. Ten samples from each group were subjected to shear bond strength evaluation. Additionally one sample per group was subjected to scanning electron microscopic analysis for observing resin-enamel interfacial adaptation.
Statistical analysis: Data collected was subjected to statistical analysis using one –way ANOVA and post-hoc tukey’s test at a significant level of p<0.05.
Results: Enamel bond strength was significantly better in etch-and-rinse mode than the self etch mode for both Single Bond Universal (gp 2 & 3; p=0.017) and Tetric N-Bond Universal adhesives (gp 4 & 5; p=0.046) respectively.
Conclusion: Phosphoric acid etching of enamel prior to the application of a mild universal adhesive is an advisable strategy for optimizing bonding.
Dental Light-Cured Polymer Nanocomposites: A Brief Review of Experimental Results Obtained from the Study of Some Physicochemical Properties
Authors: Vouvoudi Evangelia C. , Sideridou Irini D. 
Published: January 2023

This article is a brief review of the experimental results obtained from the study of some physicochemical properties of five dental commercial light-cured nanocomposites: Filtek Supreme XT Body (FSB) and Filtek Supreme XT Translucent (FST), both characterized as nanofill composites, and Grandio (GR), Tetric EvoCeram (TEC) and Protofill-nano (PR) characterized as nanohybrid composites. The properties presented are: volumetric shrinkage due to polymerization, sorption, solubility and volume increase after sorption of composites in water or artificial saliva SAGF® medium for 30 days, flexural strength and modulus after immersion in water or artificial saliva for one or 30 days and finally the dynamic mechanical properties (storage modulus E', loss modulus E'', tand=E''/E' and Tg) determined 1h after light-curing or after immersion in an oral/food simulating liquid (water, artificial saliva or ethanol/water solution 75%vol) for 1 month. It was found via physicochemical properties that the most corrosive solvent is EtOH which heavily affects the polymeric matrix, while water and saliva are smoother media. Moreover, the more rigid and concrete polymeric chains may sustain higher load during mechanical tests, so that the composites with stronger networks shall demonstrate resistance.
MgAl2O4:Mn2+ Red-Emitting Phosphor: Influence of MgF2 Flux on the Microstructure and Luminescence Properties
Authors: Li Shan , Zhao Shu-Yu , Ye Tian-Nan , Wu Xue-Yan , Wang Kai-Xue , Wei Xiao , Chen Jie-Sheng 
Published: January 2023

A serial of Mn ions doped MgAl2O4 phosphors, exhibiting only red light centred at 650 nm, have been prepared by a solid state reaction (SSR) with and without MgF2 flux. These phosphors have been characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and electron paramagnetic resonance (EPR) measurements. EPR result demonstrates that Mn ions exist as +2 valence. The emission intensity of the phosphors varies with Mn2+ concentration. The addition of MgF2 flux to the reactants enhances the luminescence properties through elevating the crystallinity, regulating the morphology and particle size of the MgAl2O4:Mn2+ phosphor. The phosphor MgAl2O4:0.10%Mn2+ with 12 wt% MgF2 flux shows the best emission performance.
Meteorological Conditions for the Variability of 7Be and 210Pb Concentrations in Surface Layer Air in Poland
Authors: Krajny Ewa , Osrodka Leszek , Wojtylak Marek 
Published: January 2023

The aim of this study was to determine the effect of meteorological factors on the concentration of beryllium 7Be and lead 210Pb in the surface layer air on background their variability due to environmental conditions. Data analysis was carried out for 13 Polish stations for the period 2005-2009. The measurement cosmogenic and terrestrial radioactivity were collected on network station type ASS-500. The analysis includes the following meteorological elements from NWS stations: temperature, precipitation, atmospheric pressure, wind speed, humidity, solar radiation and in addition number of sunspots (SSN). It was considered that these meteorological elements mainly responsible for the transport and removal processes of 7Be and 210Pb from the atmosphere. The study method was used correlation analysis. Because of the relatively small area of Poland, it is hard to prove the dependence of 7Be activity concentration variability on the influence of such factors as geographical location or SSN. However, the activity concentration of 7Be and 210Pb are dependent on meteorological conditions. The inverse interaction of atmosphere stability conditions on 7Be and 210Pb allows one to use the 7Be/210Pb ratio as an indicator of vertical transport in the atmosphere.
Path Analysis for Soil Total Nitrogen in Relation to Selected Soil Properties in a Short-Term Flooding Wetland
Authors: Gao Zhaoqin , Bai Junhong , Ding Qiuyi , Liu Guihuan , Wang Junjing , Lu Qiongqiong , Zhao Qingqing 
Published: January 2023

Soil samples were collected in a typical short-term flooding wetland with two dominate vegetation types (i.e., Phragmites australis and Suaeda salsa) of the Yellow River Delta of China. Path analysis was applied to investigate the relationship between soil nitrogen and selected 12 soil properties. Correlation analysis showed that soil total nitrogen was significantly correlated with total carbon, nitrate nitrogen, available phosphorous, total potassium, total sulfur and soil organic matter (P <0.01). Path analysis demonstrated that total carbon, nitrate nitrogen and soil organic matter had higher direct and indirect positive effects to total nitrogen, whereas bulk density and salt exhibited higher negative effects. Additionally, available phosphorous, total potassium, total sulfur exhibited indirect effects on total nitrogen through total carbon.
Freight Service Platform Ecosystems – Value Co-Creation durch Einsatz mobiler Telematik für Transportladungsträger in Plattform-Ökosystemen
Authors: Heinbach Christoph , Thomas Oliver 
Published: January 2023

ZusammenfassungDer Einsatz innovativer Informationstechnologien in Gütertransportsystemen offenbart neue Entfaltungspotenziale, um die Servitization in der Branche voranzutreiben. Neue datenbasierte Dienstleistungsangebote entstehen dabei durch Internet of Things (IoT) Plattform-Ökosysteme und ermöglichen den Informationsaustausch zwischen den beteiligten Akteuren. Diese Entwicklung wird insbesondere durch die Nutzung mobiler Telematik als Enabler-Technologie deutlich, die zunehmend für Transportladungsträger (TLT, z. B. Trailer) im B2B Bereich angewendet wird, damit intelligente Dienste entstehen, die von den Akteuren gemeinsam genutzt werden können. Vor diesem Hintergrund ermöglichen telematikgestützte TLT eine Value Co-Creation durch die Einbeziehung des Kunden, die von den einzelnen Akteuren in übergreifenden Dienstleistungsbereichen realisiert wird. In diesem Beitrag stellen die Autoren das Konzept Freight Service Platform Ecosystems (FSPEs) vor, das eine prozessorientierte Value Co-Creation durch telematikgestützte TLT in Plattform-Ökosystemen orchestriert. Basierend auf den Ergebnissen einer durchgeführten Studie wird ein Gestaltungsvorschlag für FSPEs präsentiert. Anschließend wird auf Grundlage des Value in Interaction Modells die positive Gestaltung der Werte entlang der identifizierten Dimensionen in FSPEs herausgestellt. Durch das präsentierte Konzept und die plattformbasierten Interaktionen werden abschließend strategische Handlungsmaßnahmen für gemeinsame Wertschöpfungen formuliert. Eine Value Co-Creation entsteht für die Akteure insbesondere für die Leistungsbewertung, Anomalieerkennung, Risikobewertung, Kommunikation und den Frachtinformationsaustausch, die einen vernetzten Flottenbetrieb in digitalisierten und zunehmend autonomen Transportlogistikketten unterstützen.