bluuebunny/crossref_metadata_2025_split huggingface dataset:
Domain analysis of Drosophila Blimp‐1 reveals the importance of its repression function and instability in determining pupation timing
Authors: Sarhan Moustafa , Miyagawa Koichi , Ueda Hitoshi 
Published: March 2023

AbstractThe PRDM family transcription repressor Blimp‐1 is present in almost all multicellular organisms and plays important roles in various developmental processes. This factor has several conserved motifs among different species, but the function of each motif is unclear. Drosophila Blimp‐1 plays an important role in determining pupation timing by acting as an unstable transcriptional repressor of the βftz‐f1 gene. Thus, Drosophila provides a good system for analyzing the molecular and biological functions of each region in Blimp‐1. Various Blimp‐1 mutants carrying deletions at the conserved motifs were induced under the control of the heat shock promoter in prepupae, and the expression patterns of βFTZ‐F1 and Blimp‐1 and pupation timing were observed. The results showed that the regions with strong and weak repressor functions exist within the proline‐rich middle section of the factor and near the N‐terminal conserved motif, respectively. Rapid degradation was supported by multiple regions that were mainly located in a large proline‐rich region. Results revealed that pupation timing was affected by the repression ability and stability of Blimp‐1. This suggests that both the repression function and instability of Blimp‐1 are indispensable for the precise determination of pupation timing.
Mothering load: Underlying realities of professionally engaged Indian mothers during a global crisis
Authors: Mazumdar Ketoki , Parekh Sneha , Sen Isha 
Published: March 2023

AbstractThe current global crisis has had a significant impact on professionally engaged mothers and the proliferation of the invisible nature of the work that they are engaged in on a daily basis. Several research studies have indicated how mothers seem to have been particularly affected. Mothers experienced an exacerbation in their domestic household and child‐care responsibilities due to the absence of househelp and other child care arrangements while balancing their professional careers. These challenges crystallized the existing gender inequalities and the gendered nature of parenting. Using a feminist lens, this study explores the experiences of mothering load during COVID‐19 against the backdrop of urban India. A total of two themes and six sub‐themes were identified through the process of thematic analysis—Triad of work included increased care work, increased formal work, and increased worry work and Mothering experiences: Burdens and Biases included the lack of support, parental role overload, and gendered nature of parenting. This study adds to the limited empirical evidence of working mothers in India while straddling the worlds of feminism and mental health activism. Findings indicate the need to explicitly highlight the invisibilized phenomena of unpaid care work, worry work, and the gendered nature of parenting that contribute to the larger experience of mothering load. The findings also point toward acknowledging the importance of maternal mental health and the United Nations Sustainable Development Goal 5 to establish and enforce stronger policies around recognizing and appreciating unpaid care and domestic work to promote gender equality and empowering women at all levels. This may be enacted through the encouragement of shared responsibilities within the household and family units as contextually feasible and through the development of appropriate infrastructure, social protection policies, and the delivery of public services.
Japanese subgroup analysis of GLIMMER: A global Phase IIb study of linerixibat for the treatment of cholestatic pruritus in patients with primary biliary cholangitis
Authors: Tanaka Atsushi , Atsukawa Masanori , Tsuji Keiji , Notsumata Kazuo , Suyama Akari , Ito Hiroshi , Das Sugato , von Maltzahn Robyn , McLaughlin Megan M. 
Published: March 2023

AbstractAimTo compare patient characteristics and outcomes between the overall and Japanese populations of GLIMMER.MethodsGLIMMER was a multicenter, double‐blind, randomized, placebo‐controlled, Phase IIb study evaluating linerixibat for the treatment of pruritus in patients with primary biliary cholangitis.ResultsIn total, 147 patients were randomized in the GLIMMER overall population with 38 patients comprising the Japanese population. Demographics and baseline clinical characteristics were similar across treatment groups and between both populations. A reduction in mean worst daily itch score from baseline to week 16 (primary endpoint) was seen in all groups, with the largest reduction observed with linerixibat 40 mg twice daily (BID; −2.92 [95% confidence interval: −5.07, −0.76] and −2.86 [95% confidence interval: −3.76, −1.95] for Japanese and overall populations, respectively). The highest proportion of responders was generally in the 40 mg BID group in both populations regardless of the responder definition applied. Improvements in health‐related quality of life were generally consistent in both populations. In the Japanese and overall populations, on‐treatment drug‐related adverse events were reported in 25% and 19% of patients in the placebo group and 0%–86% and 31%–78% of patients in the linerixibat groups, respectively. Consistent with the mechanism of action, the most common events were gastrointestinal in nature. The effects of linerixibat on pharmacodynamic biomarkers favored BID dosing.ConclusionsTherapeutic responses and safety of linerixibat were consistent between the Japanese and overall populations of GLIMMER. Linerixibat may provide an effective treatment option for cholestatic pruritus in patients with primary biliary cholangitis.Clinical Trial RegistrationNCT02966834.
Patient public involvement (PPI) in health literacy research: Engagement of adults with literacy needs in the co‐creation of a hospital‐based health literacy plan
Authors: McKenna Verna B. , Sixsmith Jane , Byrne Niki 
Published: March 2023

AbstractBackgroundPeople with literacy needs can experience many challenges in accessing, understanding and using health services and health information. Such challenges can adversely impact patient‐provider interactions and ultimately, health outcomes. Healthcare providers need to be aware of health literacy (HL) to address the demands of healthcare systems, improve their interactions with communities and patients and promote patient engagement for improved health outcomes.MethodsThis paper reports on a process of patient and public involvement (PPI) with participants in an adult literacy programme acting as PPI contributors to identify priority areas for a local hospital HL action plan and to develop a protocol for a PPI process with other groups. A qualitative community‐based participatory research study design informed by principles of PPI was undertaken, drawing on the tools of participatory and visual methods, open discussion and workshop format to facilitate a process of co‐creation. Three workshops with six PPI contributors took place to identify issues to be included in the hospital action plan. PPI contributors identified issues and grouped these into priority areas using discussion and ranking procedures.ResultsKey areas prioritised for HL action by the PPI contributors were: verbal communication, emphasising the patient's right to understand, and improved understanding of medication use. These were incorporated into the action plan. The workshop format and process were deemed acceptable to the group and input on improvements will be incorporated into further work in this area.ConclusionPPI acts as a lever in the knowledge translation process. Genuine engagement with service users can meaningfully contribute to relevant and sustainable changes to services as well as foster the empowerment of service users.Patient or Public ContributionMembers of the public with literacy needs actively participated in the co‐creation of a HL action plan for a local hospital and in the development of a protocol for a patient and public process for HL research.
Development of the Pharm‐SAVES educational module for gatekeeper suicide prevention training for community pharmacy staff
Authors: Stover Amanda N. , Lavigne Jill E. , Shook Alexis , MacAllister Catherine , Cross Wendi F. , Carpenter Delesha M. 
Published: March 2023

AbstractIntroductionPharmacists are one of the most accessible health professionals in the United States, who, with training, may serve as gatekeepers who recognize suicide warning signs and refer at‐risk individuals to care. Our objective was to codesign a 30‐min online gatekeeper training module (Pharm‐SAVES) specifically for community pharmacy staff.MethodsOver a period of 8 months, a nine‐member pharmacy staff stakeholder panel and the Finger Lakes (New York) Veterans Research Engagement Review Board each worked with the study team to codesign Pharm‐SAVES. Formative data from previous interviews with community pharmacists were presented to the panels and guided website development.ResultsFour key topics were identified for brief skills‐based modules that could be delivered asynchronously online. To help pharmacy staff understand their opportunities as gatekeepers in suicide prevention, statistics and statements from the Joint Commission and pharmacy professional organizations were highlighted in Module 1 (‘Why Me?’). Module 2 (‘What can I do?’) presents the five gatekeeping steps (SAVES): (1) Recognize suicide warning Signs, (2) Ask if someone is considering suicide, (3) Validate feelings, (4) Expedite referral, and (5) Set a reminder to follow‐up. Module 3 (‘How does it work?’) provides three video scenarios modeling SAVES steps and two interactive video cases for participant practice. Module 3 demonstrates use of the 24/7 National Suicide Prevention Lifeline, including the DOD/VA Crisis Line. Module 4 (Resources) includes links to national resources and a searchable zip code‐based provider directory. Pharm‐SAVES was codesigned with pharmacy and veteran stakeholders to deliver brief, skills‐focused, video‐based interactive training that is feasible to implement in busy community pharmacy settings.ConclusionPharm‐SAVES is a brief, online suicide prevention gatekeeper training program codesigned by researchers, community pharmacy and veteran stakeholders. By actively engaging stakeholders at each stage of the design process, we were able to create training content that was not only realistic but more relevant to the needs of pharmacy staff. Currently, Pharm‐SAVES is being evaluated in a pilot randomized controlled trial for changes in pharmacy staff suicide prevention communication behaviors.Patient or Public ContributionStakeholder engagement was purposefully structured to engage pharmacy staff and pharmacy consumers, with multiple opportunities for study contribution. Likewise, the involvement of patient/public contribution was paramount in study design and overall development of our study team.
How do social media influencers inspire consumers' purchase decisions? The mediating role of parasocial relationships
Authors: Ashraf Arif , Hameed Irfan , Saeed S. Amir 
Published: March 2023

AbstractIn recent years, social media influencers (SMIs) have become part of the strategic communication of firms because the traditional form of mass media communication is losing its effectiveness. Thus, it is imperative to study the effects of communication through SMIs on marketing outcomes, such as purchase intentions. Based on the fairness dimensions, that is, information, distributive, interpersonal, and procedural, and dimensions of source credibility, that is, attractiveness, expertise, trustworthiness, and similarity, this research investigated how technology‐oriented SMIs (T‐SMIs) foster parasocial relationships (PSRs) with followers. Purposive sampling was employed to collect data from 506 active social media users. Data were analysed through IBM AMOS and SPSS 26. The results of the study suggested that attractiveness, expertise, trustworthiness, similarity, interpersonal, procedural, and information fairness are positively related to fostering PSR with followers. In contrast, distributive fairness does not strengthen PSR with followers; furthermore, the study findings suggested an indirect positive influence of attractiveness, expertise, trustworthiness, similarity, interpersonal, procedural, and information fairness on the purchase intentions of consumers via PSR. This study is useful for academia and industry since it fills important gaps in the literature and provides recommendations to brand managers of technology companies about selecting appropriate SMIs for the endorsement of their brands.
The current state of family caregiver burden and support of toilet problems for elderly with mild cognitive impairment and Alzheimer's disease
Authors: Kamiya Masaki , Osawa Aiko , Shinoda Yusuke , Nishii Hisae , Kondo Izumi 
Published: March 2023

ObjectiveTo conduct an exploratory examination of caregiver burden involving toilet problems in patients with mild cognitive impairment (MCI) and Alzheimer's disease (AD) and related characteristics.MethodsWe included 50 outpatients with amnestic MCI and AD and their caregivers. Patients were subclassified into three groups: MCI, mild AD, and moderate/severe AD. We used the Japanese version of the Zarit Burden Interview (J‐ZBI) to evaluate caregiver burden and conducted a questionnaire on the frequency of lower urinary tract symptoms and related caregiver burden. We compared the frequency of questionnaire items with the level of burden in each group and subsequently determined the J‐ZBI correlation coefficient.ResultsAmong the questionnaire items, the caregiver's burden of “increased daytime urinary frequency,” “nocturia,” “urinary incontinence,” and “they cannot clean the toilet” statistically significantly correlated with J‐ZBI scores (ρ = 0.52, 0.65, 0.79, and 0.83, respectively). Items including “they cannot clean the toilet,” “the smell of excrement is bothersome,” “assistance for transfer is necessary,” “they soil the clothes and bed,” and “they cannot clean the genital region” were significantly more common in the patient group with moderate/severe AD.ConclusionsLower urinary tract symptoms and toilet problems were significantly correlated with caregiver burden. Toilet problems differ depending on the severity of dementia. Therefore, a support system based on dementia severity is required to address toilet problems.
Correlation between serum copeptin and urinary aquaporin‐2 levels in children with primary monosymptomatic nocturnal enuresis
Authors: Fathi Basem A. , El Gammal Ahmed A. , Abd Alrahim Nosaiba M. , Abd Elalim Alshaymaa A. , Abd Elhalim Eatemad N. 
Published: March 2023

ObjectivesTo determine the utility of serum copeptin and urinary aquaporin‐2 (AQP2) levels in diagnosing primary monosymptomatic nocturnal enuresis (PMNE) in children.MethodsThis study comprised 58 children (30 males and 28 females), aged 9.7 (±2.9) years with PMNE enuresis. Another 29 children (16 males and 13 females) aged 10.2 (±3.3) without nocturnal enuresis (NE) were recruited as a control group. History taking, clinical examination, and assessment of serum copeptin (blood) and AQP‐2 levels (urine) were performed in all participants.ResultsSerum levels of copeptin, potassium and urinary AQP‐2, and urine creatinine levels were lower in the PMNE group compared to the control group (p < 0.001 for all). No significant differences in body mass index, urine specific gravity, serum sodium, serum creatinine, or estimated glomerular filtration rate were observed between groups. This study evaluated both serum copeptin and AQP‐2 levels in healthy and enuretic children.ConclusionsIn this study, serum levels of copeptin (blood) and AQP2 (urine) were significantly lower in enuretic patients compared to healthy controls. Further, the measurement of urinary AQP‐2 levels is more practical than serum copeptin levels due to lower invasiveness.
An insight into population structure and genetic progress of Argentinean Holstein cattle
Authors: Pardo Alan M. , Casanova Daniel , Rubio Natalia , Andere Cecilia , Rodríguez Edgardo , Corva Pablo M. 
Published: March 2023

AbstractThe objective of the present study was to investigate the most critical issues associated with the limited genetic progress evidenced in the Argentinean Holstein (“Holando Argentino”) breed in the last 20 years (only 26% of the phenotypic trend in milk yield was due to genetics). The study comprised the analysis of population structure, realized genetic selection differentials, genetic progress and partition of genetic trends by sex and country of origin from 1936 to 2019 (1,045,582 records; 24,680 sires and 619,322 dams in the pedigree). Average inbreeding steadily increased in the last 15 generations (ΔF = 0.6%, which translates to Ne = 75). Partition of genetic trends revealed that local genetics made a negligible contribution to genetic progress, which for most traits was highly dependent on imported genetics (>80%). Mean generation intervals were fairly constant until 2009 (8–9 years for males and 5–6 years for females, respectively) and then decreased, especially in the paths of sires of bulls and dams of bulls (to 5 and 4 years, respectively) mostly due to the influence of imported sires. The reduction in generation intervals was counterbalanced by a marked deterioration of realized selection differentials, particularly in the path of sires of bulls that nevertheless made the largest contribution to genetic progress. In the last 20 years, realized selection differentials in this path went from 533.6 to 170.8 kg for milk yield and from 16.7 to 13.3 kg for protein yield (1.7–0.5 and 1.6–1.3 standard deviation units, respectively). Among all considered traits (milk yield, fat yield, protein yield, stature, final score and daughter pregnancy rate) in the analysed period, annual genetic gain was negative for milk yield, fairly constant for composition and conformation traits, and positive only in the case of daughter pregnancy rate. Considered together, these results suggest that limited genetic progress is due to the absence of a sound breeding programme that includes genomic selection and a carefully defined selection objective, together with the absence of stronger regulations in germplasm importation; however, other factors such as potential genetics by environment interactions cannot be ruled out.
Distribution of excitation recoverable myocardium after radiofrequency ablation and its relation to energy application time and irrigation
Authors: Saitoh Osamu , Kasai Takumi , Fuse Kyogo , Chinushi Masaomi 
Published: March 2023

AbstractIntroductionRadiofrequency (RF) catheter ablation induces excitation recoverable myocardium around durable core lesions, and its distribution may be different depending on energy delivery methods.Methods and ResultsIn coronary perfusing porcine hearts, pacing threshold through the ventricle was measured using eight‐pole (1‐mm distance) needle electrodes vertically inserted into myocardium before, within 3 min after and 40 min after 40 W ablation with 10‐g catheter contact (Group 1: irrigation catheter for 15 s, Group 2: irrigation catheter for 40 s, Group 3: nonirrigation catheter for 15 s, Group 4: nonirrigation catheter for 40 s). Ablation was accomplished in all 12 ablations in Groups 1–3 whereas in 8/12 ablations in Group 4 because of high‐temperature rise. Within 3 min after ablation, 10.0 V pacing uncaptured electrodes were distributed from the surface to inside the myocardium, and its depth was deeper in 40 s than in 15 s ablation. 40 min after ablation, excitation recovery at one or more electrodes below the durable lesion was observed in all Groups. Excitation recovery electrodes were also observed on the surface in Group 1 but not the other Groups. Accordingly, the number of excitation‐recovered electrodes were larger in Group 1 than the other Groups.ConclusionsRegardless of the ablation methods, excitation recoverable myocardium was present around 1.0 mm below the durable lesions. Lesions created by short application time using an irrigation catheter may have included large excitation recoverable myocardium soon after ablation because of the presence of reversible myocardium on well‐irrigated myocardial surfaces.
Ambient circulation surrounding an ablation catheter tip affects ablation lesion characteristics
Authors: Nussinovitch Udi , Wang Paul , Babakhanian Meghedi , Narayan Sanjiv M. , Viswanathan Mohan , Badhwar Nitish , Zheng Lijun , Sauer William H. , Nguyen Duy T. 
Published: March 2023

AbstractIntroductionThe association between ambient circulating environments (CEs) and ablation lesions has been largely underexplored.MethodsViable bovine myocardium was placed in a saline bath in an ex vivo endocardial model. Radiofrequency (RF) ablation was performed using three different ablation catheters: 3.5 mm open irrigated (OI), 4, and 8 mm. Variable flow rates of surrounding bath fluids were applied to simulate standard flow, high flow, and no flow. For in vivo epicardial ablation, 24 rats underwent a single OI ablation and performed with circulating saline (30 ml/min; n = 12), versus those immersed in saline without circulation (n = 12).ResultsHigh flow reduced ablation lesion volumes for all three catheters. In no‐flow endocardial CE, both 4 mm and OI catheters produced smaller lesions compared with standard flow. However, the 8 mm catheter produced the largest lesions in a no‐flow CE. Ablation performed in an in vivo model with CE resulted in smaller lesions compared with ablation performed in a no‐flow environment. No statistically significant differences in steam pops were found among the groups.ConclusionA higher endocardial CE flow can decrease RF effectiveness. Cardiac tissue subjected to no endocardial CE flow may also limit RF for 4 mm catheters, but not for OI catheters; these findings may have implications for RF ablation safety and efficacy, especially in the epicardial space without circulating fluid or in the endocardium under varying flow conditions.
Comparison of three different approaches to very high‐power short‐duration ablation using the QDOT‐MICRO catheter
Authors: Yamaguchi Junji , Takigawa Masateru , Goya Masahiko , Martin Claire A. , Yamamoto Tasuku , Ikenouchi Takashi , Shigeta Takatoshi , Nishimura Takuro , Tao Susumu , Miyazaki Shinsuke , Sasano Tetsuo 
Published: March 2023

AbstractBackground/ObjectivesThe QDOT‐MICRO™ catheter allows very high‐power and short‐duration (vHPSD) ablation. This study aimed to investigate lesion characteristics using different ablation settings.MethodsRadiofrequency applications (90 W/4 s, temperature‐control mode with 55°C or 60°C target) were performed in excised porcine myocardium using three different approaches: single (SA), double nonrepetitive (DNRA), and double repetitive applications (DRA). Applications were performed with an interval of 1 min for DNRA, and without interval for DRA.ResultsA total of 480 lesions were analyzed. Lesion depth and volume were largest for DRA followed by DNRA and SA regardless of catheter direction (depth: 3.8 vs. 3.3 vs. 2.6 mm, p < .001 for all comparisons; volume: 176.6 vs. 145.1 vs. 97.0 mm3, p < .001 for all comparisons). Surface area was significantly larger for DRA than for SA (45.1 vs. 38.3 mm2, p < .001) and larger for DNRA than for SA (44.5 vs. 38.3 mm2, p < .001), but was similar between DRA and DNRA (45.1 vs. 44.5 mm2, p = .54). Steam‐pops more frequently occurred for DRA than for SA (15.6% vs. 4.4%, p = .004) and DNRA (15.6% vs. 6.9%, p = .061), but the incidence was similar between SA and DNRA (4.4% vs. 6.9%, p = 1). Although surface area and lesion volume were larger in lesions with steam‐pops than without steam‐pops (46.5 vs. 38.1 mm2, p = .018 and 128.3 vs. 96.8 mm3, p = .068, respectively), lesions were not deeper (pop(+): 2.5 mm vs. pop(−): 2.6 mm, p = .75).ConclusionsDNRA produces larger lesions than SA without increasing the risk of steam‐pops. DRA produces the largest lesions among the three groups, but with an increased risk of steam‐pops. Even with steam‐pops, lesions do not become deeper in vHPSD ablation.