bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pulp regeneration by transplantation of dental pulp with the synergy of concentrate growth factor: An in vitro and in vivo study
Authors: Xie Yuhong , Feng Xingrong , Hu Yuntong , Wang Zhi , Xia Xueling , Luo Xinxiao , Xiao Yuhong 
Published: March 2023

Abstract

Objectives This study aimed to investigate the mechanism of successful autologous pulp transplantation through semi-in situpulp regeneration in animal experiments and three case reports of autologous pulp transplantation protocols using concentrated growth factor (CGF)-enriched pulp. Material and methods Wisdom tooth pulp was removed, placed in the anterior molar canal, and implanted in the subcranial space of the cranial apex of SD rats to establish an animal model of hemi-in situpulp regeneration. Postoperative histological observations were performed. Three patients diagnosed with chronic periapical inflammation in a single canal of the anterior teeth and satisfied with the presence of wisdom teeth were recruited, and the CGF-rich autologous pulp transplantation protocol was selected after obtaining informed consent. Result Animal experiments showed no detachment of all SD rat grafts after surgery, a large amount of neovascularization by HE staining, and positive vascular expression by immunohistochemistry for both human CD31 and murine CD31. The three patients were followed at 3 and 6 months after surgery, and all teeth showed improvement in periapical lesions and positive pulp electrical vitality tests. Conclusion The results of animal experiments indicate that isolated pulp can survive and establish a blood supply with the host, and the addition of CGF facilitates regenerative pulp formation. The clinical results also demonstrated that CGF-rich autologous pulp transplantation protocols are a good regenerative pulp therapy (RET) for the treatment of chronic apical periodontitis in anterior teeth.
Organoid modeling reveals the tumorigenic potential of the alveolar progenitor cell state
Authors: Kim Carla , Li Jingyun , Dang Susanna , Schurmann Paul , Dost Antonella , Moye Aaron , Paschini Margherita , Bhetariya Preetida , Bronson Roderick , Sui Shannan Ho 
Published: March 2023

Abstract
Alveolar type 2 (AT2) cells, the epithelial progenitor cells of the distal lung, are known to be the prominent cell of origin for lung adenocarcinoma. The regulatory programs that control chromatin and gene expression in AT2 cells during the early stages of tumor initiation are not well understood. Here, we dissected the response of AT2 cells to Kras activation and p53 loss (KP) using combined single cell RNA and ATAC sequencing in an established tumor organoid system. Multi-omic analysis showed that KP tumor organoid cells exhibit two major cellular states: one more closely resembling AT2 cells (SPC-high) and another with loss of AT2 identity (hereafter, Hmga2-high). These cell states are characterized by unique transcription factor (TF) networks, with SPC-high states associated with TFs known to regulate AT2 cell fate during development and homeostasis, and distinct TFs associated with the Hmga2-high state. CD44 was identified as a marker of the Hmga2-high state, and was used to separate organoid cultures for functional comparison of these two cell states. Organoid assays and orthotopic transplantation studies indicated that SPC-high cells have higher tumorigenic capacity in the lung microenvironment compared to Hmga2-high cells. These findings highlight the utility of understanding chromatin regulation in the early oncogenic versions of epithelial cells, which may reveal more effective means to intervene the progression of Kras-driven lung cancer.
Prevalence of depression among elderly women in India- An intersectional analysis of the Longitudinal Ageing Study in India (LASI), 2017-2018
Authors: Panda Paramjot , Dash Prashansa , Behera Manas , Mishra Trupti 
Published: March 2023

AbstractEpidemiological transition in India shows a shift in disease burden from youth to the elderly. As Life Expectancy increases, a greater burden is placed on the state, society, and families in India. Mental health disorders are insidious, debilitating Non-Communicable Diseases (NCDs) that afflict people, their families, and generations down the line. Globally, depression is the leading cause of mental health-related disability. It is estimated that mental illness contributes to 4.7% of Disability Adjusted Life Years (DALYs) in India. It is predicted that by 2026, the elderly's sex ratio will increase to 1,060 feminizing ageing. Research has shown that elderly women in developed countries like the United States are more prone to depression. Chronic morbidities are more common in women than in men, and they may suffer from poor vision, depression, impaired physical performance, and elder abuse. Mostly widowed, economically dependent, lacking proper food and clothing, fearing the future, and lacking proper care, they have difficulty coping with these health problems. There are surprisingly few studies on elderly female depression. Therefore, we want to hypothesize the prevalence of depression among women in different regions and demographic groups in India, and what factors may contribute to these differences. Using intersectional analysis with the data from Wave 1 (2017-2018) of the (Longitudinal Ageing Study in India) LASI (N = 16,737) we were able to explore the intersecting patterns between different variables and how people are positioned simultaneously and position themselves in different multiple categories based on the type of place of residence, age and level of education. Through the study we further aim to determine the prevalence of depression among elderly female in the age group of 60 in different states using the Chloropleth map. The findings of the study highlight the significance of the place of residence in the development of depression among elderly women, with the rural area being associated with a higher prevalence of depression compared to urban area. When compared to people with higher literacy, those with low literacy were significantly associated with depression. State-wise, there is a huge difference between the prevalence of elderly women depression in rural and urban areas. The study highlights the vulnerability of elderly women to depression. It is possible for the government to develop programs that address the needs of elderly women, both in urban and rural areas, to reduce depression. Multi-factor approaches to mental health, which consider age, literacy, and location, are essential. Programs targeting specific populations can be developed to address depression's root causes..
Zinc oxide nanoparticles induce renal injury by initiating oxidative stress, mitochondrial damage and apoptosis in renal tubular epithelial cells
Authors: Liu Shuang , Shi Yang , Zhou Han , Li Jingyan , Yi Simeng , Liao Bin , Cao Ji-Min , Li Guang 
Published: March 2023

Abstract

Zinc oxide nanoparticles (ZnO NPs) are widely used in many fields due to their unique physicochemical properties. However, the renal toxicity of ZnO NPs and the underlying mechanisms has not been well elucidated. Here we found that ZnO NPs induced injury of human renal proximal tubular epithelial cells (HK-2) in a dose- and size-dependent manner, as revealed by CCK-8, LDH and AnnexinV-FITC assay. Mechanistically, ZnO NPs promoted oxidative stress and mitochondrial damage by generating ROS, and finallyinduced apoptosis of HK-2 cells, as evidenced by upregulation of Bax and Caspase 3 and downregulation of Beclin 1. In vivo, ZnO NPs induced tubular epithelial cell apoptosis and elevations of serum creatinine, serum urea nitrogen, and urinary protein in mice suggesting damage of renal structure and function. Collectively, this study demonstrated that oral intake of ZnO NPs induced nephrotoxicity both in vitro and in vivo mainly via inducing oxidative stress, mitochondrial damage and apoptosis in the tubular epithelial cells.
Graph Convolutional Networks based Non-Negative Matrix Factorization aware Community detection
Authors: Scholar Pawan Meena Research , Professor Mahesh Pawar Associate , Professor Anjana Pandey Associate 
Published: March 2023

Abstract
Nonnegative matrix factorization (NMF) is widely used in community discovery because of its effectiveness and easy interpretability. However, most of the existing NMF-based community detection methods are linear. They cannot effectively deal with the nonlinear characteristics of complex networks, resulting in further improvement in community detection performance. Aiming at this problem, a convolution graph network (GCN) enhanced nonlinear NMF community discovery method NMFGCN is proposed. NMFGCN consists of two main modules: GCN and NMF, where GCN is used to learn network node representations, and NMF uses node representations as input to obtain network community representations. In addition, a joint optimization method is proposed to train NMFGCN, which enables NMFGCN to have nonlinear feature representation capabilities and enables GCN and NMF to promote each other and obtain better community segmentation results. Many experiments on artificial synthetic networks and entire networks show that NMFGCN is superior to current NMF-based community discovery methods , thus proving that NMFGCN can improve the performance of NMF community discovery methods. In addition, NMFGCN also outperforms Deep Walk and LINE standard graph representation learning methods.
College Students' Suicidal Behavior: Protective and Risk Factors in a State University in Pampanga, Philippines
Authors: Bangalan Sherna , Otanes Marie Eugenie , Escalona Arsenio 
Published: March 2023

Abstract
Background
The world's fourth leading cause of death among young people is a suicide, a serious public health concern. In the Philippines, there is an increasing number of suicide deaths.
Objective
The present study aims to investigate the prevalence of suicidal behavior among college students in a state university in Pampanga, Philippines, and examine the risk and protective factors most associated with suicidal behavior.
Method
A cross-sectional study used data from 522 college students obtained through an online survey; 443 were included in the final analysis. Six (6) standardized scales were used to assess the study variables. 24% of the participants have suicidal ideation, 14% have suicidal plans, and 9% would attempt to commit suicide.
Results
A multiple logistic regression analysis revealed that family support and spiritual well-being were protective factors against suicide attempts. On the contrary, depressive symptoms and adverse childhood experiences increased the likelihood of the participants committing suicide.
Discussion
Even though an association does not imply causation, suicide prevention programs and policies would benefit from understanding how family support, spiritual well-being, and suicidal behavior are interconnected. Moreover, treating depressive symptoms and adverse childhood experiences should be integrated into targeted mental health interventions to reduce suicidal behavior.
The Values of k-th Order of Entire Functions Share 1 CM with its Linear Differential Polynomial
Authors: Wei Huanhuan , Hu Wenju 
Published: March 2023

Abstract
In this paper, we discuss the values of k-th order of the entire functions which with its linear differential polynomial share 1 CM. We prove that if k(≥ 3) is an integer, for the given non-negative real number λ (may be infinity), then there exists an entire function ƒ(z) which with its linear differential polynomial share 1 CM, such that the k-th order of ƒ(z) is λ.
MSC Classification: 30D35 , 30D20
The feasibility of adding wood quality traits as selection criteria in the Galician Pinus pinaster Aiton breeding program: case study
Authors: TORRES-SÁNCHEZ Esteban , PRADA Eva , DÍAZ Raquel 
Published: March 2023

Abstract
Pinus pinaster is a very important species for the Galician wood industry. A genetic breeding program was started in the 1980s to select plus trees based on growth and straightness. In this study, we estimated genetic parameters, juvenile-mature correlations and genetic gains in basic density (BD) and the dynamic modulus of elasticity (MOEd) in Galician breeding families, as well as their relation to volume, straightness, and tree slenderness. All traits were measured at a tree age of 12 years in three half-sib progeny trials. Wood quality traits were also measured at 24 years in two other trials. All progeny trials followed a randomized complete block design and the data were analyzed using several mixed-model approaches. The individual heritability of MOEd remained stable at both ages (~ 0.39) but decreased with age for BD (from 0.44 at age 12 to 0.24 at age 24). The high juvenile-mature correlations (0.51 for BD, 0.57 for MOEd) observed support the viability of early selection for both traits at 12 years. Both wood quality traits correlated positively with each other and weakly with volume, straightness and tree slenderness. Selecting a minimum number of the best families, based on volume or any wood quality trait, would generate genetic gains for the selecting trait and prevent genetic losses of non-selecting traits. We also identified specific families showing positive genetic gains for all traits. The research indicates that either or both of these wood quality traits could be included as new selection criteria in the Galician breeding program.
Rapid genotyping of plant samples using TaqMan TM assays
Authors: Hicks Leannah K. , Fili Stavroula , Walker Elsbeth L. 
Published: March 2023

Abstract
Insertional mutagenesis is a routine method used in reverse genetics, but commonly-used end-point PCR molecular screening methods are flawed because they rely on negative results and require labor intensive gDNA extractions. To address this, we have developed TaqMan™ genotyping assays that allow us to compare the copy number of specific alleles of a gene of interest to an internal control. The method is effective with ‘on-the-fly’ template prep with no purification that allows for streamlined genotyping of up to 95 samples in ~ 2 hours. TaqMan™ genotyping has been effective in both identifying all possible genotypes (wild type, heterozygous, and homozygous) for insertional mutants and for determining transgene copy numbers of individual transformation events in transgenic lines. Expression of maize transposon-insertion alleles was determined and the correlation between the transposon’s insertion location and its ability to effect gene expression was examined.
Extremal parameter for double phase problem with concave-convex nonlinearity
Authors: Mishra Pawan Kumar , Silva Kaye , Tripathi Vinayak Mani 
Published: March 2023

Abstract
Note: Please see pdf for full abstract with equations.
In this work, we study the following problem
−Δpu − div(μ(x)|∇u|q−2∇u) = λf(x)|u|γ−2u + g(x)|u|r−2u in Ω,
u = 0 on ∂Ω,
where Ω ⊂ RN,N ≥ max{2, p} is a bounded smooth domain, 1 < γ < p < q < r < p∗ = Np/(N−p) and λ is a positive real parameter. The weights f, g : Ω → R are continuous and bounded functions, where g may change sign on Ω. The function 0 ≤ μ ∈ Cc(Ω) ∩ L∞(Ω) and μ ≢ 0. The objective of this work is to explore the optimal control on λ to apply Nehari manifold
Mathematics Subject Classification (2010). Primary 35A02; Secondary 35A15, 35B32.
Stability Analysis of a Fractional Order of a New Corona Virus Disease (Covid-19) Model
Authors: Labzai Abderrahim , Belam Mohamed , Rachik Mostafa 
Published: March 2023

Abstract
In this paper, we propose a fractional order SEIHR pandemic model of Covid-19 that describesthe spreadability of Corona virus within a population. We prove the global existence, positivity, and bound-edness of solutions. In addition, the local and the global stability of the disease-free equilibrium and thedisease equilibrium are studied by using Routh-Hurwitz criteria and construction an appropriate Lyapunovfunctions. Finally, numerical simulations are performed to verify the theoretical results.
The conservation status of the Cretan Endemic Arthropods under Natura 2000 network
Authors: Bolanakis Giannis , Paragkamian Savvas , Chatzaki Maria , Kotitsa Nefeli , Kardaki Liubitsa , Trichas Apostolos 
Published: March 2023

Abstract

Arthropods' decline has been documented in global and local studies, yet they are still not sufficiently protected on a global scale. Crete (Greece), a Mediterranean hotspot is a continental island, renowned for its diverse geology, ecosystems and endemicity of flora and fauna, with many studies on all of the above disciplines dating back to the 19th century. Here we investigate the conservation status of the Cretan Arthropods using Preliminary Automated Conservation Assessments (PACA) and the overlap of Cretan Arthropod distributions with the Natura 2000 protected areas. In order to perform this analysis, we assembled occurrences of the endemic Arthropods in Crete located in the collections of the Natural History Museum of Crete together with literature data. These assessments resulted in 75% of endemic Arthropods as potentially threatened. The hotspots of endemic and threatened taxa are distributed mostly on the mountainous areas where the Natura 2000 protected areas have great coverage. Yet human activities have significant impact even in those areas, while some taxa are not sufficiently covered by Natura 2000. These findings call for countermeasures and conservation actions.
Menger curve and Spherical CR uniformization of a closed hyperbolic 3-orbifold
Authors: Ma jiming , Xie Baohua 
Published: March 2023

Abstract

Let G6,3= ⟨a0, · · · , a5|a3i = id, aiai+1 = ai+1ai, i ∈ Z/6Z⟩ be a hyperbolic group with boundary the Menger curve. Granier [10] constructed a discrete, convex cocompact and faithful representation p of G6,3 into PU(2,1). We show the 3-orbifold at infinity of p(G6,3 )is a closed hyperbolic 3-orbifold, with underlying space the 3-sphere and singular locus the Z_3-coned chain-link C(6,-2). This answers the second part of Kapovich's Conjecture 10.6 in [14], and it also provides the second explicit example of a closed hyperbolic 3-orbifold that admits uniformizable spherical CR-structure after Schwartz's first example in [25].
Curcumin and omega-3 ameliorate experimental osteoarthritis progression in terms of joint pain and mitochondrial dysfunction
Authors: Jhun JooYeon , Lee Donghwan , Na Hyun Sik , Cho Keun-Hyung , Lee Seung Yoon , Lee Jeong Su , Kim Seok Jung , Cho Mi-La , Park Sung-Hwan 
Published: March 2023

Abstract
Background
Osteoarthritis (OA), a chronic degenerative disorder, induces pain, joint inflammation, and destruction of the articular cartilage matrix. Curcumin and omega-3 have been used as dietary supplements for OA due to their anti-inflammatory and antioxidant properties. However, there is no evidence demonstrating a synergistic effect in OA. The current study aimed to investigate the therapeutic effects and underlying mechanism of a combination of curcumin and omega-3 in the treatment of OA.
Methods
Wistar rats were injected with monosodium iodoacetate to induce OA. Oral treatments of a vehicle, curcumin, curcumin and omega 3, or celecoxib were administered. Pain was analyzed according to the paw withdrawal latency, paw withdrawal threshold, and weight bearing ability. The joint was isolated from OA rats, and cartilage damage was evaluated using histomorphological techniques, the Mankin scoring system, and micro computed tomography analysis. Protein expression in the joint was examined using immunohistochemistry. The expression levels of catabolic markers were measured in curcumin and omega-3-treated OA chondrocytes.
Results
The OA animal model revealed diminished pain and cartilage conservation in response to the combined treatment. mRNA levels of matrix metalloproteinase 1 (MMP1), MMP3, and MMP13 were reduced in interleukin-1 beta-simulated human OA chondrocytes. Additionally, mitochondrial markers, cytochrome c oxidase 4, and TOMM20, were increased by the combination treatment.
Conclusions
These findings suggest promising therapeutic outcomes for the combined treatment of curcumin and omega-3 in OA patients.
Knowledge Framework Construction, Question Bank Promotion and Case Collection Driving on Grade Point Average and the Medical Licensing Examination
Authors: Qu Xiaoying , Cao Jinfeng , Huang Xiaoming , Li Zhuoquan 
Published: March 2023

Abstract
Objectives:Passing the medical licensing exam is a primary objective of MBBS (Bachelor of Medicine and Bachelor of Surgery) education and a major milestone in medical careers. This study aimed to explore the influence of various learning methods on the participation and pass rate of the license exam.
Methods: Three different study tactics were employed among two grades (A: n=15 and B: n=23) of MBBS students. These tactics were Knowledge framework construction (KFC), Question bank promotion (QBP) and Case collection driving (CCD).
Results: The percentage distribution of KFC, QBP and CCD at the 1st AY in grade A was 40.0%, 40.0% and 20.0%, respectively, while at the 6th AY, it was 33.3%, 40.0% and 26.7%. At the 1st AY in grade B, the distribution was 52.2%, 21.7% and 26.1%, and at the 6th AY, it was 13.1%, 47.8% and 39.1%. The overall participation and pass rates in grade A were higher than in grade B (73.3% to 78.3%, 53.3% to 60.9%). The pass rates of participants in KFC, QBP and CCD in grade A were 50.0%, 100.0%, 66.7%, respectively, and in grade B were 0.0%, 100.0%, 57.1%. The grade point average (GPA) was significantly higher in QBP (4.2 ± 0.4) than in KFC (3.8 ± 0.2), but not in CCD (3.9 ± 0.4).
Conclusions: The pass rate of MBBS students in the license exam was higher in grade A than in grade B, and the study method of QBP had a better effect on the passing rate and GPA than KFC and CCD.
Distal Radius Reconstruction Using Proximal Non-Vascularized Fibula Graft in a Patient during the Syrian Conflict: A Case Study on Treatment Outcomes
Authors: Khatib Mohamad , Hasani Ibrahim 
Published: March 2023

Abstract
Giant cell tumor (GCT) is a bone tumor that is encountered frequently and can have both benign and aggressive characteristics. It is commonly found in individuals between the ages of 20 and 45, with a slightly higher incidence in females. A 22-year-old female presented with a progressively increasing painful and tender lump in the distal forearm, along with restricted wrist movements and inability to use her right hand. The patient's medical history was complicated by the ongoing Syrian conflict, which limited her access to medical care and resources. Physical examination showed a large lump in the distal radius and severely restricted pronation and supination, additionally, Plain radiographs and computerized tomography (CT) were suggestive of an aggressive giant cell tumor (GCT) of the proximal humerus, which was confirmed on histopathological examination. After appropriate investigation, we decided to proceed with En bloc resecting and reconstructing the resultant defect with a proximal non-vascularized fibula graft. Despite the challenges posed by the Syrian conflict, our surgical intervention successfully restored the patient's arm function and provided her with a positive outcome. This case highlights the importance of adapting to difficult circumstances in order to provide necessary medical care to patients affected by conflict and other adverse situations.
Horizontal Mergers and Innovation with Knowledge Spillovers
Authors: Hwangbo Changwoo , Lee Jihong 
Published: March 2023

Abstract
We introduce knowledge spillovers in a model of innovation competition à la Federico et al. (2017) and Denicolò and Polo (2018), which otherwise features horizontal mergers that harm innovation due to the business stealing effect. With knowledge spillovers, competition discourages investment in R&D due to free-riding, and by internalizing such externalities, a merger can improve the incentives for innovation. Horizontal mergers raise (reduce) innovation if the spillover effect is large (small), in contrast with the standard account of spillover effects on mergers.
JEL codes: G34, L13, L40, O30