bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotypic and genotypic detection of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in Accra, Ghana
Authors: Dwomoh Felicia P. , Kotey Fleischer C. N. , Dayie Nicholas T. K. D. , Osei Mary-Magdalene , Amoa-Owusu Felicia , Bannah Vida , Alzahrani Fuad M. , Halawani Ibrahim F. , Alzahrani Khalid J. , Egyir Beverly , Donkor Eric S. 
Published: December 2022

Aim
To describe the occurrence of carbapenem resistance among multidrug-resistant (MDR) Escherichia coli and Klebsiella pneumoniae isolated from clinical specimens in Accra using phenotypic and genotypic methods.

Methodology
The study was cross-sectional, involving 144 clinical MDR E. coli and K. pneumoniae isolates recovered from the Central Laboratory of the Korle Bu Teaching Hospital (KBTH). The isolates were re-cultured bacteriologically, identified using standard biochemical tests, and subjected to antibiotic susceptibility testing using the Kirby-Bauer method. Carbapenem resistance was determined based on imipenem, meropenem, and ertapenem zones of inhibition, as well as minimum inhibitory concentrations (MICs). Carbapenemase production was determined phenotypically by modified Hodge test (MHT) and modified carbapenem inactivation method (mCIM), and genotypically with multiplex PCR targeting the blaKPC, blaIMP, blaNDM, blaVIM, and blaOXA-48 genes.

Results
Of the 144 MDR isolates, 69.4% were E. coli, and 30.6% were K. pneumoniae. The distribution of antimicrobial resistance rates among them was ampicillin (97.2%), cefuroxime (93.1%), sulfamethoxazole-trimethoprim (86.8%), tetracycline (85.4%), cefotaxime and cefpodoxime (77.1% each), amoxicillin-clavulanate (75%), ceftriaxone (73.6%), ciprofloxacin (70.8%), levofloxacin (66.0%), cefepime (65.3%), ceftazidime (64.6%), gentamicin (48.6), piperacillin-tazobactam (40.3%), cefoxitin (14.6%), amikacin (13.9%), ertapenem and meropenem (5.6% each), and imipenem (2.8%). In total, 5.6% (8/144) of them were carbapenem-resistant (carbapenem MIC range = 0.094–32.0 μg/ml), with 75% (6/8) of these testing positive by the phenotypic tests and 62.5% (5/8) by the genotypic test (of which 80% [4/5] carried blaOXA-48 and 20% (1/5) blaNDM). The blaVIM, blaIMP, and blaKPC genes were not detected.

Conclusion
Although the rates of antibiotic resistance among the isolates were high, the prevalence of carbapenemase producers was low. The finding of blaOXA-48 and blaNDM warrants upscaling of antimicrobial resistance surveillance programmes and fortification of infection prevention and control programmes in the country.
A flashing light may not be that flashy: A systematic review on critical fusion frequencies
Authors: Lafitte Alix , Sordello Romain , Legrand Marc , Nicolas Virginie , Obein Gaël , Reyjol Yorick 
Published: December 2022

Background
Light pollution could represent one of the main drivers behind the current biodiversity erosion. While the effects of many light components on biodiversity have already been studied, the influence of flicker remains poorly understood. The determination of the threshold frequency at which a flickering light is perceived as continuous by a species, usually called the Critical Fusion Frequency (CFF), could thus help further identify the impacts of artificial lighting on animals.

Objective
This review aimed at answering the following questions: what is the distribution of CFF between species? Are there differences in how flicker is perceived between taxonomic classes? Which species are more at risk of being impacted by artificial lighting flicker?

Methods
Citations were extracted from three literature databases and were then screened successively on their titles, abstracts and full-texts. Included studies were critically appraised to assess their validity. All relevant data were extracted and analysed to determine the distribution of CFF in the animal kingdom and the influence of experimental designs and species traits on CFF.

Results
At first, 4881 citations were found. Screening and critical appraisal provided 200 CFF values for 156 species. Reported values of CFF varied from a maximum of between 300 Hz and 500 Hz for the beetle Melanophila acuminata D. to a mean of 0.57 (± 0.08) Hz for the snail Lissachatina fulica B. Insects and birds had higher CFF than all other studied taxa. Irrespective of taxon, nocturnal species had lower CFF than diurnal and crepuscular ones.

Conclusions
We identified nine crepuscular and nocturnal species that could be impacted by the potential adverse effects of anthropogenic light flicker. We emphasize that there remains a huge gap in our knowledge of flicker perception by animals, which could potentially be hampering our understanding of its impacts on biodiversity, especially in key taxa like bats, nocturnal birds and insects.
Influence of metformin intake on serum vitamin B12 levels in patients with type 2 diabetes mellitus
Authors: Al-Fawaeir Saad , Al-Odat Ibrahim 
Published: December 2022

Objective
To describe the prevalence of vitamin B12 deficiency among Jordanian patients with type 2 diabetes mellitus treated with metformin and to compare the findings with those who did not receive metformin.

Design and method
Total 155 patients with type 2 diabetes mellitus, aged between 48 and 82 years were enrolled in the current study. They were divided into two groups; the first (n = 120) was treated with metformin while the second (n = 35) was not. Patients’ demographics (age, gender, duration of type 2 diabetes mellitus, smoking status), medication parameters (daily dosage and duration of metformin therapy), and biochemical parameters (hemoglobin level, mean corpuscular volume (MCV), serum vitamin B12, and folate level) were recorded. Definite deficiency was defined as serum vitamin B12 levels of < 150 pg/ml, whereas < 200 pg/ml indicated possible deficiency.

Results
The mean serum ± standard deviation (SD) vitamin B12 level was significantly lower in patients who were treated with metformin (268.5 ± 35.8 vs. 389.5 ± 29.8 pg/ml, p = 0.029). The metformin group had significantly higher prevalence of definite deficiency (32% vs. 9%, p < 0.02) and possible deficiency (48% vs. 30%, p < 0.02). Within the metformin group, the mean serum ± SD vitamin B12 level was significantly lower in those on high dosage (175.2 ± 30.5 vs. 315.6 ± 37.8 pg/ml, p < 0.001). MCV (μm3) levels ± SD were higher in the metformin group (87.5 ± 2.9 vs. 83.7 ± 2.4) with no statistical significance.

Conclusion
There is a significant association between metformin intake and vitamin B12 deficiency. Serum vitamin B12 levels should be checked by physicians and serial monitoring is necessary in patients who are treated with metformin.
Uptake of appointment spacing model of care and associated factors among stable adult HIV clients on antiretroviral treatment Northwest Ethiopia
Authors: Asrade Abaynew Assemu , Moges Nurilign Abebe , Meseret Maru , Alemu Kasaye Demeke , Tsega Tilahun Degu , Petrucka Pammla , Telayneh Animut Takele 
Published: December 2022

Introduction
Ethiopia launched an Appointment Spacing Model in 2017, which involved a six-month clinical visit and medication refill cycle. This study aimed to assess the uptake of the Appointment Spacing Model of care and associated factors among stable adult HIV clients on ART in Ethiopia.

Methods
A cross-sectional study was conducted from October 3 to November 30, 2020 among 415 stable adult ART clients. EpiData version 4.2 was used for data entry and SPSS version 25 was used for cleaning and analysis. A multivariable logistic regression model was fitted to identify associated factors, with CI at 95% with AOR being reported to show the strength of association.

Results
The uptake of the appointment spacing model was 50.1%. Residence [AOR: 2.33 (95% CI: 1.27, 4.26)], monthly income [AOR: 2.65 (95% CI: 1.13, 6.24)], social support [AOR: 2.21 (95% CI: 1.03, 4.71)], duration on ART [AOR: 2.41 (95% CI: 1.48, 3.92)], baseline regimen change [AOR: 2.20 (95% CI: 1.02, 4.78)], viral load [AOR: 2.80 (95% CI: 1.06, 7.35)], and alcohol abstinence [AOR: 2.02 (95% CI: 1.21, 3.37)] were statistically significant.

Conclusions
The uptake of the ASM was low. Behavioral change communication, engaging income-generating activities, and facility-level service providers’ training may improve the uptake.
Education and employment status among adults with Loeys-Dietz syndrome and vascular Ehlers-Danlos syndrome in Norway, a questionnaire based study
Authors: Johansen Heidi , Velvin Gry , Lidal Ingeborg B. 
Published: December 2022

Objectives
To describe education level and employment status among adults with Loeys-Dietz syndrome and vascular Ehlers-Danlos syndrome, and explore factors related to work participation.

Materials and methods
Cross-sectional postal survey in 2018. Individuals with molecularly verified diagnosis were recruited through a National Resource Centre for Rare Disorders. A study specific questionnaire included topics on disease burden and validated instruments regarding education level, employment, pain, fatigue, psychological distress, and satisfaction with life.

Results
Fifty persons (56% women) aged 18–67 years, participated. Almost 60% reported education level ≤13 years. Two thirds (66%) received disability benefits, 21 (42%) had full-time disability pension. The median age at ending work was 41 years. Full-time employed and students were younger (p = 0.014), less fatigued (p = 0.035), had less sleep problems (p = 0.028) and higher satisfaction with life (p<0.001) than those who received disability pension. A third (32%) were currently or used to be in sedentary work, and 68% currently had or used to be in practical work requiring much standing and walking (23%), much walking and lifting (34%) or heavy manual work (11%).

Conclusions
There is a potential that more adults with these diagnoses can sustain employment for more years. Health and social service follow-up routines and future studies should include details on employment perspectives to reveal those at risk of poor employment and to identify modifiable factors for work participation.
Association between myopia progression and quantity of laser treatment for retinopathy of prematurity
Authors: Hwang Eileen S. , Kassem Iris S. , Allozi Rawan , Kravets Sasha , Al-Kirwi Khalid Y. , Hallak Joelle A. , Costakos Deborah M. 
Published: December 2022

Background
Previous studies found that infants with retinopathy of prematurity (ROP) who were treated for more posterior disease with a greater number of laser spots developed higher myopia. These studies included multiple physicians with variations in laser density. In treatments by a single physician, laser spot count is a better surrogate for area of avascular retina and anterior-posterior location of disease, so that the relationship with myopia can be better assessed.

Methods
Our retrospective study included infants treated with laser for ROP by a single surgeon at a single center. Exclusion criteria were irregularities during laser and additional treatment for ROP. We assessed correlation between laser spot count and change in refractive error over time using a linear mixed effects model.

Results
We studied 153 eyes from 78 subjects treated with laser for ROP. The average gestational age at birth was 25.3±1.8 weeks, birth weight 737±248 grams, laser spot count 1793±728, and post-treatment follow up 37±29 months. Between corrected ages 0–1 years, the mean spherical equivalent was +0.4±2.3 diopters; between ages 1–2, it was -1.3±3.2D; and ages 2–3 was -0.8±3.1D. Eyes that received more laser spots had significantly greater change in refractive error over time (0.30D more myopia per year per 1000 spots). None of the eyes with hyperopia before 18 months developed myopia during the follow-up period.

Conclusions
Greater myopia developed over time in infants with ROP treated by laser to a larger area of avascular retina.
Cardiac strain is lower among women with HIV in relation to monocyte activation
Authors: Toribio Mabel , Awadalla Magid , Drobni Zsofia D. , Quinaglia Thiago , Wang Melissa , Durbin Claudia G. , Alagpulinsa David A. , Fourman Lindsay T. , Suero-Abreu Giselle Alexandra , Nelson Michael D. , Stanley Takara L. , Longenecker Christopher T. , Burdo Tricia H. , Neilan Tomas G. , Zanni Markella V. 
Published: December 2022

Background
Women with HIV (WWH) face heightened risks of heart failure; however, insights on immune/inflammatory pathways potentially contributing to left ventricular (LV) systolic dysfunction among WWH remain limited.

Setting
Massachusetts General Hospital, Boston, Massachusetts.

Methods
Global longitudinal strain (GLS) is a sensitive measure of LV systolic function, with lower cardiac strain predicting incident heart failure and adverse heart failure outcomes. We analyzed relationships between GLS (cardiovascular magnetic resonance imaging) and monocyte activation (flow cytometry) among 20 WWH and 14 women without HIV.

Results
WWH had lower GLS compared to women without HIV (WWH vs. women without HIV: 19.4±3.0 vs. 23.1±1.9%, P<0.0001). Among the whole group, HIV status was an independent predictor of lower GLS. Among WWH (but not among women without HIV), lower GLS related to a higher density of expression of HLA-DR on the surface of CD14+CD16+ monocytes (ρ = -0.45, P = 0.0475). Further, among WWH, inflammatory monocyte activation predicted lower GLS, even after controlling for CD4+ T-cell count and HIV viral load.

Conclusions
Additional studies among WWH are needed to examine the role of inflammatory monocyte activation in the pathogenesis of lower GLS and to determine whether targeting this immune pathway may mitigate risks of heart failure and/or adverse heart failure outcomes.

Trial registration
Clinical trials.gov registration: NCT02874703.
The Immersive Enclosure: Virtual Reality in Japan, Paul Roquet (2022)
Authors: Wohar Natalia 
Published: December 2022

Review of: The Immersive Enclosure: Virtual Reality in Japan, Paul Roquet (2022)
New York: Columbia University Press, 254 pp.,
ISBN 978-0-23120-534-4, h/bk, $140.00
ISBN 978-0-23120-535-1, p/bk, $35.00
ISBN 978-0-23155-596-8, e-book, $16.99
Numerical investigation of the effect of ocean depth variations on the dynamics of a ship mounted two-DoF manipulator system
Authors: Ramadiansyah Mohamad Luthfi , Yazid Edwar , Ng Cheng Yee 
Published: December 2022

The dynamics of a ship need to be considered in the development of a manipulator system that will be applied to the ocean-based operation. This paper aims to investigate the effect of ocean depth variations on the ship motion as disturbances to a ship-mounted two-DoF (Degrees of Freedom) manipulator joint torque using an inverse dynamics model. Realization is conducted by deriving the mathematical model of a two-DoF manipulator system subject to six-DoF ship motion, which is derived by using Lagrange-Euler method. It is then combined with numerical hydrodynamic simulation to obtain the ship motions under ocean depth variations, such as shallow (50 m), intermediate (750 m), and deep (3,000 m) waters. Finding results show that randomness of the ship motions appears on the manipulator joint torque. In the azimuth link, maximum joint torque is found in shallow water depth with an increment of 8.271 N.m (285.69 %) from the undisturbed manipulator. Meanwhile, the maximum joint torque of the elevation link is found in intermediate water depth with an increment of 53.321 N.m (6.63 %). However, the difference between depth variations is relatively small. This result can be used as a baseline for sizing the electrical motor and developing the robust control system for the manipulator that is mounted on the ship by considering all ocean depth conditions.
THE DISCOVERY OF CHALCORANA MEGALONESA IN JAVA AND THE EVALUATION OF ITS TAXONOMIC STATUS
Authors: Sabinhaliduna Efendi , Arisuryanti Tuty , Hamidy Amir 
Published: December 2022

Chalcorana chalconota was firstly described as Hyla chalconotus Schlegel from Java, and widely recognized as Rana chalconota Boulenger. This ranid frog species has widespread distribution in Southeast Asia. Molecular studies revealed that Rana chalconota is a complex species and consisted of several cryptic species. Among Sundaland population, this group was split into five species (C. chalconota sensu stricto, C. parvaccola, C. rufipes, C. raniceps, C. megalonesa), and together with C. labialis and C. eschatia, they nested in a monophyletic group. During herpetological survey in West Java, we collected two morphotypes of Rana chalconota from Pasir Angin, Bogor and evaluated their taxonomy status using morphology approach and molecular data with 16S rRNA mitochondrial gene. The results revealed two morphotypes belong to C. chalconota sensu stricto and C. megalonesa. The genetic distance of C. megalonesa between Java population and Sarawak (Borneo) population ranged from 2.4 to 2.6% that still below the threshold of species delimitation on the 16S rRNA gene. Therefore, these two populations are conspecific. This discovery of C. megalonesa in Java has contributed to increase the number of species of amphibians in Java to become 46 species.
TRADE AND THREAT ON ORNITHOPTERA CROESUS (LEPIDOPTERA: PAPILIONIDAE), THE WALLACE’S BIRDWING BUTTERFLY IN NORTH MALUKU, INDONESIA
Authors: Peggie Djunijanti 
Published: December 2022

Ornithoptera croesus, Wallace’s Golden Birdwing butterfly, is one of the most highly-demanded butterflies in the world. Annual trades of this endemic species have been monitored and mechanisms to control the trades have been developed to ensure the sustainability of the species. This paper aims to address and clarify the issues on the trade and threat to the species. The methodology involves the probe into the CITES Trade Database, into the threat to the species based on observation during field surveys, and into the possible solution. The results showed that there were some discrepancies in the data provided by the exporter and importer of the species and that the threats to the species are real. An approach to improve the condition is discussed. The solution to meet the demand for this species is offered through captive breeding and ranching for sustainable use.
Sharenting of Young Mothers in Yogyakarta: A Phenomenological Study
Authors: Hasanah Fitria Fauziah , Ermawati Mela 
Published: December 2022

This research is motivated by the rising phenomenon of young mothers who do sharenting. Sharenting is a phenomenon of parents' habit of sharing information about their children on social media. This study aimed to identify descriptions sharenting by young mothers in Yogyakarta. This study employed descriptive qualitative research, collecting data through interviews, observations, and recordings. The data analysis technique in this study adopts phenomenological research analysis. Findings suggest that sharenting among young mothers includes young mothers sharing using social media accounts to make information about their children more accessible to others; young mothers sharenting without regard for anonymous sharing; and young mothers accidentally exposing their children's real whereabouts or full names; young mothers don't give their children veto rights over online creations; young mothers accidentally share pictures of their children undressing; and young mothers don't think the sharenting effect will affect their children's present and future state.
Keywords: Sharenting, Young mothers, Early Childhood.
An examination of some physiological and anthropometric characteristics of 10-12 year-old soccer players
Authors: Söğüt Tarkan , Akkuş Hasan 
Published: December 2022

Research aim: This study aimed to examine some physiological and anthropometric characteristics of 10-12 years old soccer players. Method: 20 subjects aged 11.05±0.94, who have been actively participating in Konya Besiktas Football Schools for 3 years, voluntarily participated in the study. These subjects’ body weight, height, body fat percentage, arm span, leg length, bust length, back strength, leg strength, hand grip strength, anaerobic power, maximum oxygen consumption and 30 m sprint test measurements were made. Averages and standard deviations of the measured and calculated values were analysed using the “IBM SPSS Statistics Version v.21” statistical program. Results: As a result of the tests and measurements made, the subjects average body weight was 37.31±7.71 kg, height 147.66±9.25 cm, body fat percentage 17.99±3.92%, arm span 145.4±10,1 cm, leg length 69.62±6.57 cm, bust length 76.51±3.4 cm, back strength 51.95±12.86 kg, leg strength 52.77±14.36 kg, hand grip strength 17.67±5.34 kg, anaerobic power value 46.07±12.22 kgm/sec, maximum oxygen consumption (maxVO2) 44.61±3.23 ml/kg/min, and 30 m sprint test score 5.74±0.32 sec. Discussion and Conclusion: As a result of the literature review, it was determined that children in the same age group have similar physiological and anthropometric characteristics.
RELATIONSHIP BETWEEN ANXIETY LEVEL IN PANDEMIC AND MEDICAL ADHERENCE IN HYPERTENSIVE ELDERLY
Authors: Affandy Haidar Yusuf 
Published: December 2022

Abstract Background: Hypertension in Indonesia increases with age. People with hypertension need continuous treatments according to doctor’s recommendation, if not it will cause serious complications. Medication non-adherence of hypertensive elderly caused by many factor, one of them is psychological factor. Health care visits decrease during pandemic because of the COVID-19 transmission anxiety.Objective: To find out the correlation between anxiety level and medical adherence of hypertensive elderly in Kangkung Village, Mranggen during the COVID-19 pandemic.Methods: Cross-sectional study was conducted with Proportional Random Sampling approach on 96 hypertensive elderly in Kangkung, Mranggen, Demak Regency. The study was conducted by interview using Zung Self-rating Anxiety Scale and Morisky Medical Adherence Questionnaire. Data analysis using Rank Spearman correlation test. This study had got Ethical Clearance from Health Research Ethics Committee of Faculty of Medicine of Diponegoro University.Results: There was a correlation between anxiety level and medical adherence, p value = 0,005 (p < 0,01) and the higher anxiety level will decrease the medical adherence (ρ = – 0,287).Conclusion: Anxiety that were experienced by the elderly has been shown to be related to medical adherence during the COVID-19 pandemic.
Elementy magii i religii w rzeczywistości społecznej kapitalizmu
Authors: Kufliński Mateusz 
Published: December 2022

The article is dedicated to the issue of magic and religion in the social reality of capitalism. The goal of the article is to present examples proving that capitalism in various places (post-communist Central Europe, San Cristobal in the Mexican state of Chiapas, Colombian department Valle del Cauca, New York) and at various times (from the 1970s to the second decade of the 21st century) was associated with elements of religion and magic through social thinking and practice, and therefore elements of social reality. On the other hand, in the discourse, capitalism is often presented as a part of nature: the existence that acts objectively, like the laws of physics. In the article, this apparent contradiction is presented as a dialectical aspect of the present day, in which the discourse and social reality are complementary.
Escape room na lekcji języka obcego
Authors: Łagowska Kinga 
Published: December 2022

The idea of implementing an escape room as part of foreign language teaching fits perfectly with the needs of today's learner, where we create a natural learning environment using digital tools. The escape room, whether stationary or virtual, gives space for the use of different types of language tasks. The article first describes the escape room in the light of theoretical considerations. It then presents the results of a survey, conducted among 31 teachers of various foreign languages. The aim of the survey was to gather feedback from the teachers who themselves use escape rooms in their language classes.