bluuebunny/crossref_metadata_2025_split huggingface dataset:
ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis
Authors: Yang Min , Chen Yanping , Huang Xueshan , Shen Fang , Meng Yanni 
Published: July 2023

Abstract
Purpose
Bronchopulmonary dysplasia (BPD) is associated with hyperoxia-induced oxidative stress-associated ferroptosis. This study examined the effect of E26 oncogene homolog 1 (ETS1) on oxidative stress-associated ferroptosis in BPD.

Methods
Hyperoxia-induced A549 cells and neonatal mice were used to establish BPD models. The effects of ETS1 on hyperoxia-induced ferroptosis-like changes in A549 cells were investigated by overexpression of ETS1 plasmid transfection and erastin treatment. Glucose consumption, lactate production, and NADPH levels were assessed by the glucose, lactate, and NADP+/NADPH assay kits, respectively. The potential regulatory relationship between ETS1 and Nrf2/HO-1 was examined by treating hyperoxia-induced A549 cells with the Nrf2 inhibitor ML385. ETS1 effect on the Nrf2 promoter was explored by dual-luciferase reporter and chromatin immunoprecipitation assay. The effect of ETS1 on the symptoms of BPD mice was examined by injecting an adenovirus overexpressing ETS1.

Results
ETS1 overexpression increased hyperoxia-induced cell viability, glucose consumption, lactate production, and NADPH levels and reduced inflammation and apoptosis in A549 cells. In animal experiments, ETS1 overexpression prevented weight loss, airway enlargement, and reductions in radial alveolar counts in BPD mice, while reducing the mean linear intercept, mean alveolar diameter and inflammation. ETS1 overexpression suppressed PTGS2 and CHAC1 expression, reduced ROS, MDA and ferrous iron (Fe2+) production and increased GSH levels in hyperoxia-induced A549 cells and BPD mice. In addition, ETS1 can bind to the Nrf2 promoter region and thus promote Nrf2 transcription. ETS1 overexpression increased the mRNA and protein levels of Nrf2, HO-1, xCT, and GPX4 in hyperoxia-induced A549 cells and BPD mice. In hyperoxia-induced A549 cells, erastin and ML385 treatment abolished the effect of ETS1 overexpression.

Conclusion
ETS1 is important in oxidative stress-related ferroptosis in a hyperoxia-induced BPD model, and the effect is partially mediated by the Nrf2/HO-1 axis.
Exploring factors associated with higher education students’ learning outcomes in emergency remote teaching environments during the COVID-19 pandemic: General patterns and individual differences
Authors: Zou Yuqing , Zou Chunrui 
Published: July 2023

AbstractIn this study, we aimed to explore the factors associated with higher education students’ learning outcomes in emergency remote teaching environments (ERTEs) during the COVID-19 pandemic at both the population and individual levels, given the limited understanding in previous research. 9418 students from 41 countries were selected for analysis from a survey-based dataset that was collected with the aim of understanding the self-perceived impacts of the first-wave COVID-19 pandemic on higher education students. We conducted structural equation modeling to explore associated factors and latent profile analysis to identify student profiles based on these factors. Utilizing the identified profiles, we developed a random forest-based classifier to identify the membership of students’ profiles. The results showed that six environmental and individual factors—partially mediated by academic emotions—were significantly associated with learning outcomes. The positively associated factors, ranked by path coefficient, were satisfaction with synchronous course organization (SCO), computer skills (CS), satisfaction with asynchronous course organization (ACO), satisfaction with administration support (AS), and perceived teacher support (TS). The negatively associated factor was worry about life. Based on these factors, eight profiles were identified with varying learning outcomes and student characteristics. The classifier achieved a testing accuracy of 0.904. By integrating variable-centered and person-centered approaches, this study bridges the gap in understandings of general patterns and individual differences regarding key factors associated with higher education students’ learning outcomes. The findings have implications for designing individualized interventions and support strategies to enhance student learning outcomes and mitigate educational disparities in ERTEs during crisis situations.
Incidence, prevalence and mortality of chronic liver diseases in Sweden between 2005 and 2019
Authors: Nasr Patrik , von Seth Erik , Mayerhofer Raphaela , Ndegwa Nelson , Ludvigsson Jonas F. , Hagström Hannes 
Published: July 2023

Abstract
Background
Updated data on the incidence, prevalence, and regional differences of chronic liver disease are missing from many countries. In this study, we aimed to describe time trends, incidence, prevalence, and mortality of a wide range of chronic liver diseases in Sweden.

Methods
In this register-based, nationwide observational study, patients with a register-based diagnosis of chronic liver disease, during 2005–2019, were retrieved from the Swedish National Board of Health and Welfare. Annual age-standardized incidence and mortality rates, and prevalence per 100,000 inhabitants was calculated and stratified on age, sex, and geographical region.

Results
The incidence of alcohol-related cirrhosis increased by 47% (2.6% annually), reaching an incidence rate of 13.1/100,000 inhabitants. The incidence rate of non-alcoholic fatty liver disease and unspecified liver cirrhosis increased by 217% and 87% (8.0 and 4.3% annually), respectively, reaching an incidence rate of 15.2 and 18.7/100,000 inhabitants, and a prevalence of 24.7 and 44.8/100,000 inhabitants. Furthermore, incidence rates of chronic hepatitis C declined steeply, but liver malignancies have become more common. The most common causes of liver-related mortality were alcohol-related liver disease and unspecified liver disease.

Conclusion
The incidence rates of diagnosed non-alcoholic fatty liver disease, alcohol-related cirrhosis, unspecified liver cirrhosis, and liver malignancies have increased during the last 15 years. Worryingly, mortality in several liver diseases increased, likely reflecting increasing incidences of cirrhosis in spite of a decreasing rate of hepatitis C. Significant disparities exist across sex and geographical regions, which need to be considered when allocating healthcare resources.
Highlighting Water-Bearing Zones in Tunnel by Inverse Wavefield Transform of TEM Data
Authors: Wang Gang , Wang Neng , zheng Xin , Fan Kerui 
Published: July 2023

AbstractTransient electromagnetic method (TEM) is an effective tool to map the groundwater distribution in tunnel construction. To highlight the structural information of the water-bearing zones, inverse wavefield transform is used to extract pseudo-wavefield from TEM data. To provide accurate and reliable pseudo-wavefield, pseudo-wavefield extraction should tackle the large dynamic range of the kernel function, ill-posed inverse transform function, and self-adaptive solving. In this paper, a series of works had been done to solve the above problems. Based on the property of the wavefield transform and its kernel function, we provided an empirical formula to shorten the integral interval of the inverse wavefield transform. A self-adaptive method combined with the precise integral method (PIM) was conducted to provide accurate equation solving. The data misfit between the modeling data and the fitted data is less than 2.5% in our numerical models. The traces of the pseudo-wavefield highlight the boundary of the low-resistivity layers of different depths and thicknesses. For the field data measured in a constructing tunnel, the reflection in our pseudo-wavefield traces provided massive subsurface electrical interfaces with a data misfit of less than 5%. Combined with the low-resistivity zones in the apparent resistivity results, three water-bearing zones were highlighted and then verified by a drilling test, which confirms the reliability and practicality of our method.
Also for k-means: more data does not imply better performance
Authors: Loog Marco , Krijthe Jesse H. , Bicego Manuele 
Published: July 2023

AbstractArguably, a desirable feature of a learner is that its performance gets better with an increasing amount of training data, at least in expectation. This issue has received renewed attention in recent years and some curious and surprising findings have been reported on. In essence, these results show that more data does actually not necessarily lead to improved performance—worse even, performance can deteriorate. Clustering, however, has not been subjected to such kind of study up to now. This paper shows that k-means clustering, a ubiquitous technique in machine learning and data mining, suffers from the same lack of so-called monotonicity and can display deterioration in expected performance with increasing training set sizes. Our main, theoretical contributions prove that 1-means clustering is monotonic, while 2-means is not even weakly monotonic, i.e., the occurrence of nonmonotonic behavior persists indefinitely, beyond any training sample size. For larger k, the question remains open.
No longer able to or no longer wanting to? Are intention violations failures to exert or decisions not to exert self-control?
Authors: Kilger Helena , Reis Dorota , Friese Malte 
Published: July 2023

AbstractSeveral theoretical models describe two pathways linking self-control demands with subsequent goal violations. The volitional pathway suggests that these goal violations should be interpreted as failures, while the motivational pathway suggests an interpretation as decisions. In this article, we examined (a) which psychological processes may explain the relationship between self-control demands and subsequent intention violations and (b) to what extent these violations reflect self-control failures rather than deliberate decisions. Results of two experience sampling studies showed that facing demands can trigger two opposing processes: fatigue, which leads to more subsequent violations of intentions, and the feeling that one deserves a reward, which leads to fewer subsequent violations of intentions due to boosts in self-efficacy. The actor may attribute intention violations to either an inability to act otherwise (indicating an actual failure) or a deliberate decision (indicating no failure). The different attributions have marked implications for the cognitive and affective downstream consequences of violating one’s goals, pointing to the importance of distinguishing between actual and apparent failures in self-control.
The Utility of CYP2D6 and CYP2C19 Variants to Guide Pharmacological Treatment in Complex Unipolar Major Depression: A Pilot Longitudinal Study
Authors: Ramaraj Reshma , Al-Mahayri Zeina N. , Saleous Reema , Abdel Aziz Karim , Al-Mugaddam Fadwa , Al-Sabousi Mouza , Alhassani Aysha , Ahbabi Noura Ali Al , Stip Emmanuel , Patrinos George P. , Ali Bassam R. , Arnone Danilo 
Published: July 2023

AbstractMajor depression is a frequent condition which variably responds to treatment. In view of its high prevalence, the presence of treatment resistance in major depression significantly impacts on quality of life. Tailoring pharmacological treatment based on genetic polymorphisms is a current trend to personalizing pharmacological treatment in patients with major depressive disorders. Current guidelines for the use of genetic tests in major depression issued by the Clinical Pharmacogenomics Implementation Consortium (CPIC) are based on CYP2D6 and CYP2C19 polymorphisms which constitute the strongest evidence for pharmacogenomic guided treatment. There is evidence of increased clinical response to pharmacological treatment in major depression although largely in non-treatment resistant patients from Western countries. In this study, well characterised participants (N = 15) with complex, largely treatment resistant unipolar major depression were investigated, and clinical improvement was measured at baseline and at week-8 after the pharmacogenomics-guided treatment with the Montgomery Åsberg Depression Rating Scale (MÅDRS). Results suggested a statistically significant improvement (p = 0.01) of 16% at endpoint in the whole group and a larger effect in case of changes in medication regime (28%, p = 0.004). This small but appreciable effect can be understood in the context of the level of treatment resistance in the group. To our knowledge, this is the first study from the Middle East demonstrating the feasibility of this approach in the treatment of complex major depressive disorders.
Effect of Salmonella pathogenicity island 1 and 2 (SPI-1 and SPI-2) deletion on intestinal colonization and systemic dissemination in chickens
Authors: Pico-Rodríguez Jwerlly Tatiana , Martínez-Jarquín Hugo , Gómez-Chávez José de Jesús , Juárez-Ramírez Mireya , Martínez-Chavarría Luary Carolina 
Published: July 2023

AbstractSalmonella’s virulence genes are located in two regions known as Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2). SPI-1 allows the bacteria to invade the intestine, while SPI-2 is important for intracellular survival and replication, although it is also necessary for intestinal disease. The aim of this study was to evaluate the effect of the deletion of SPI-1 or SPI-2 genes on the intestinal and systemic salmonellosis using the avian model. Groups of chickens were orally infected with 1010 Colony-Forming Units (CFU) of S. Typhimurium SL1344 WT strain, as well as mutants ∆SPI-1 or ∆SPI-2. At different times post-infection, 5 chickens from each group were euthanized and examined postmortem. Cecum and liver were taken from each chicken for determination of CFU’s, histopathological analysis and immunochemistry. Bacterial colonies were recovered from the liver and cecum samples infected with WT strain, while in the cultures from the organs infected with the mutant strains no colonies were recovered or were drastically affected in the ability to survive. In histopathological analysis, the WT strain produced lesions in liver and ceca, and it was detected by immunohistochemistry throughout the course of the infection. On the other hand, organs of chickens infected with ∆SPI-1 or ∆SPI-2 showed attenuated lesions and the immunohistochemistry revealed less bacteria compared to the WT strain. Taken together, our results show the importance of SPI-1 and SPI-2 genes for the complete intestinal and systemic disease in an in vivo avian model.
Obstructive sleep apnea treatment improves temporomandibular disorder pain
Authors: Alessandri-Bonetti Anna , Lobbezoo Frank , Mangino Gilda , Aarab Ghizlane , Gallenzi Patrizia 
Published: July 2023

Abstract
Purpose
The existence of a bidirectional relationship between poor sleep and pain intensity has been studied, and good sleep quality has been found to be a key factor underlying pain control. The purpose of this prospective cohort study was to observe if OSA treatment provides a reduction in temporo-mandibular disorder (TMD) pain and headache attributed to TMD in patients with obstructive sleep apnea (OSA) after 18 months of OSA treatment.

Methods
A prospective cohort study was conducted on consecutive patients suffering from OSA. Patients underwent polysomnography and TMD examination according to the DC/TMD protocol at baseline and after 18 months. Intensity of TMD pain and headache attributed to TMD were analyzed.

Results
Of 40 patients (31 men, mean age 51.3 ± 10.3 years), 33 underwent OSA treatment. At the follow-up examination after 18 months, significant improvements in the intensity of pain-related TMD and headache attributed to TMD were observed (p < 0.05). Seven patients did not start treatment for OSA or discontinued treatment. These patients did not show any significant difference in intensity of TMD-pain or headache attributed to TMD after 18 months (p > 0.05).

Conclusions
Significant reductions in intensity of pain-related TMD and headache attributed to TMD were observed in patients with OSA after 18 months of OSA treatment onset, while no difference was observed in subjects not undergoing or discontinuing OSA treatment.

Trial registration
The protocol was registered on ClinicalTrials.gov database with number NCT04948541.
Integrative Analysis of Inflammatory Response-Related Gene for Predicting Prognosis and Immunotherapy in Glioma
Authors: Zhao Zhen , Zheng Baoping , Zheng Jianglin , Zhang Yi , Jiang Cheng , Nie Chuansheng , Jiang Xiaobing , Yao Dongxiao , Zhao Hongyang 
Published: July 2023

AbstractInflammatory response plays a crucial role in the development and progression of gliomas. Whereas the prognostic esteem of inflammatory response-related genes has never been comprehensively explored in glioma, the RNA-seq information and clinical data of patients with glioma were extracted from TCGA, CGGA, and Rembrandt databases. The differentially expressed genes (DEGs) were picked out between glioma tissue and non-tumor brain tissue (NBT). Then, the least absolute shrinkage and selection operator (LASSO) regression analysis was performed to construct the prognostic signature in the TCGA cohort and verified in other cohorts. Kaplan–Meier survival analyses were conducted to compare the overall survival (OS) between the high and low-risk groups. Univariate and multivariate Cox analyses were subsequently used to confirm the independent prognostic factors of OS, and then, the nomogram was established based them. Furthermore, immune infiltration, immune checkpoints, and immunotherapy were also probed and compared between high and low-risk groups. The four genes were also analyzed by qRT-PCR, immunohistochemistry, and western blot trials between glioma tissue and NBT. The 39 DEGs were identified between glioma tissue and NBT, of which 31 genes are associated to the prognosis of glioma. The 8 optimal inflammatory response-related genes were selected to construct the prognostic inflammatory response-related signature (IRRS) through the LASSO regression. The effectiveness of the IRRS was verified in the TCGA, CGGA, and Rembrandt cohorts. Meanwhile, a nomogram with better accuracy was established to predict OS based on the independent prognostic factors. The IRRS was highly correlated with clinicopathological features, immune infiltration, and genomic alterations in glioma patients. In addition, four selective genes also verified the difference between glioma tissue and NBT. A novel prognostic signature was associated with the prognosis, immune infiltration, and immunotherapy effect in patients with gliomas. Thus, this study could provide a perspective for glioma prognosis and treatment.
The Rule of Law in Cities of the Medieval Low Countries: Community-Building in Context
Authors: De ruysscher D. 
Published: July 2023

AbstractUrban communities were established in the twelfth and thirteenth century with the aid of legal concepts that comprised early notions of the rule of law. Cities were envisaged as “communes”, which referred to popular sovereignty. In a first period, urban citizenship was flexible and closely related to place of residence. From around 1220 this model came under increasing pressure. In order to safeguard the interests of the most affluent citizens, large guilds were established. Status determined rights, and there were significant inequalities even among citizens. Ideas of democratic democracy and the civic virtues of citizenship were fostering reforms after 1250. Existing urban governments were expanded to include councils and burgomasters. A framework of checks and balances developed because the commune, now considered as the body of citizens and residents, was seen as a force coexisting with metropolitan institutions. The medieval examples show that, in response to economic and even global conditions, community-building and rule-of-law thinking were solutions offering a “unity-in-diversity”.
Service quality and business performance: the mediating role of innovation
Authors: Kankam George 
Published: July 2023

AbstractLiterature is replete with studies indicating how innovation influences service quality and business performance. In addition to highlighting the research trends in the service sector under investigation, this paper explores the impact factor of innovation on service quality leading to business performance. The cross-sectional survey was the research approach used, while confirmatory factor analysis and structural equation modeling (CB-SEM) was used to analyze the data. In order to study the mediation effect of innovation on service quality and business performance among restaurants bootstrap was used to conduct mediation analysis. The study highlighted that innovation fully mediates service quality and business performance among restaurants in Ghana. Moreover, a business model that highlighted the effectiveness of innovation to service quality leading to business performance among restaurants in Ghana is being advocated. This study will encourage restaurant owners to use innovation in their daily routine to improve service to improve their company’s success.
Establishing best practices in the use of an upgraded airborne teaching laboratory
Authors: Daniels S. , Braithwaite G. , Gratton G. 
Published: July 2023

AbstractSince the 1980s National Flying Laboratory Centre has used the Jetstream family of aircraft as a flying classroom, providing university students and developing professionals with real-world exposure to theoretical concepts in the form of practical flight test instruction. Recently the Jetstream was replaced with a newer Saab-340B. The work in this paper presents an experimental analysis of instruction using the Jetstream, compared with known best practices, to inform its replacement process. Flight activities were observed, and participating students (n = 60) were surveyed at four set intervals to establish their mood and interest towards the module. A pen and paper test, comparing what participants learned compared to a controlled group was also administered. While the module was still able to excite, motivate and re-contextualise previously taught information to students, upgrades to the aging technology suite, specifically to support data analysis and briefing was one of the greatest needs from the newer aircraft.
Hindsight2020: Characterizing Uncertainty in the COVID-19 Scientific Literature
Authors: Dobolyi Kinga , Sieniawski George P. , Dobolyi David , Goldfrank Joseph , Hampel-Arias Zigfried 
Published: July 2023

Abstract
Following emerging, re-emerging, and endemic pathogen outbreaks, the rush to publish and the risk of data misrepresentation, misinterpretation, and even misinformation puts an even greater onus on methodological rigor, which includes revisiting initial assumptions as new evidence becomes available. This study sought to understand how and when early evidence emerges and evolves when addressing different types of recurring pathogen-related questions. By applying claim-matching by means of deep learning Natural Language Processing (NLP) of coronavirus disease 2019 (COVID-19) scientific literature against a set of expert-curated evidence, patterns in timing across different COVID-19 questions-and-answers were identified, to build a framework for characterizing uncertainty in emerging infectious disease (EID) research over time. COVID-19 was chosen as a use case for this framework given the large and accessible datasets curated for scientists during the beginning of the pandemic. Timing patterns in reliably answering broad COVID-19 questions often do not align with general publication patterns, but early expert-curated evidence was generally stable. Because instability in answers often occurred within the first 2 to 6 mo for specific COVID-19 topics, public health officials could apply more conservative policies at the start of future pandemics, to be revised as evidence stabilizes.