bluuebunny/crossref_metadata_2025_split huggingface dataset:
Refined read-out: The hUHRF1 Tandem-Tudor domain prefers binding to histone H3 tails containing K4me1 in the context of H3K9me2/3
Authors: Choudalakis Michel , Kungulovski Goran , Mauser Rebekka , Bashtrykov Pavel , Jeltsch Albert 
Published: July 2023

AbstractUHRF1 is an essential chromatin protein required for DNA methylation maintenance, mammalian development and gene regulation. We investigated the Tandem-Tudor domain (TTD) of human UHRF1 that is known to bind H3K9me2/3 histones and is a major driver of UHRF1 localization in cells. We verified binding to H3K9me2/3 but unexpectedly discovered stronger binding to H3 peptides and mononucleosomes containing K9me2/3 with additional K4me1. We investigated the combined binding of TTD to H3K4me1-K9me2/3vs. H3K9me2/3, engineered mutants with specific and differential changes of binding, and discovered a novel read-out mechanism for H3K4me1 in an H3K9me2/3 context that is based on the interaction of R207 with the H3K4me1 methyl group and on counting the H-bond capacity of H3K4. Individual TTD mutants showed up to 10,000-fold preference for the double modified peptides, suggesting that after a conformational change, WT TTD could exhibit similar effects. The frequent appearance of H3K4me1-K9me2 regions demonstrated in our TTD pulldown and ChIP-western blot data suggests that it has specific biological roles. Chromatin pull-down of TTD from HepG2 cells and ChIP-seq data of full-length murine UHRF1 correlate with H3K4me1 profiles indicating that the H3K4me1-K9me2/3 interaction of TTD influences chromatin binding of full-length UHRF1. We demonstrated the H3K4me1-K9me2/3 specific binding of UHRF1-TTD to enhancers and promoters of cell-type specific genes, at the flanks of cell-type specific transcription factor binding sites, and provided evidence supporting an H3K4me1-K9me2/3 dependent and TTD mediated down-regulation of these genes by UHRF1, illustrating the physiological function of UHRF1-TTD binding to H3K4me1-K9me2/3 double marks in a cellular context.
Mutual dependence of Osbp and PI4KII in the maturation of regulated secretory granules
Authors: Ma Cheng-I Jonathan , Brill Julie A. 
Published: July 2023

SummarySecretory granules (SGs) are crucial for normal animal physiology due to their role in regulated exocytosis of biologically active molecules. SG membranes are enriched in phosphatidylinositol 4-phosphate (PI4P) and cholesterol, and previous studies suggest lipid composition is important for SG biogenesis and function. Nonetheless, the molecular details of how lipids are regulated during SG biogenesis remain poorly understood. Here, we identify Oxysterol binding protein (Osbp) as a novel regulator of SG biogenesis in aDrosophilamodel. We show Osbp expression level positively correlates with SG size and that Osbp requires type II phosphatidylinositol 4-kinase (PI4KII) to increase SG size. Moreover, Osbp is needed for proper PI4KII and PI4P distribution, autophagic resolution and formation of cholesterol-rich endosomal tubules that are positive for PI4KII. Feeding larvae food supplemented with sterol leads to partial suppression of SG size and PI4P distribution defects inOsbpmutants. Our results indicate that reciprocal regulation of Osbp and PI4KII drives formation of membrane tubules that mediate SG maturation through elevating PI4P levels on SG membranes.Graphical AbstractHighlightsOsbp is needed for formation of PI4KII-positive endosomal tubules that enrich secretory granule membranes with PI4P and facilitate granule maturation.Reciprocally, PI4KII is needed for Osbp-mediated secretory granule growth in salivary gland cells.Changes in Osbp expression levels alter autophagy initiation and autophagosome resolution in salivary gland cells.PI4KII-positive endosomal tubules are enriched in sterols, and sterol feeding suppresses defects caused by loss of Osbp.
A conserved graft formation process inPicea abiesandArabidopsis thalianaidentifies the PAT gene family as central regulators of wound healing
Authors: Feng Ming , Zhang Ai , Nguyen Van , Bisht Anchal , Almqvist Curt , Veylder Lieven De , Carlsbecker Annelie , Melnyk Charles W. 
Published: July 2023

AbstractThe widespread use of plant grafting has enabled eudicots and gymnosperms to join with closely related species and grow as one. Gymnosperms have dominated forests for over 200 million years and despite their economic and ecological relevance, we know little about how they graft. Here, we developed a micrografting method in conifers using young tissues that allowed efficient grafting between closely related species and distantly related genera. Conifer graft junctions rapidly connected vasculature and differentially expressed thousands of genes including auxin and cell wall-related genes. By comparing these genes to those induced duringArabidopsis thalianagraft formation, we found a common activation of cambium, cell division, phloem and xylem-related genes. A gene regulatory network analysis inPicea abies(Norway spruce) predicted thatPHYTOCHROME A SIGNAL TRANSDUCTION 1(PAT1) acted as a core regulator of graft healing. This gene was strongly upregulation during bothP. abiesandArabidopsisgrafting, andArabidopsismutants lackingPAT-genes failed to attach tissues or successfully graft. ComplementingArabidopsisPAT mutants with theP. abies PAT1homolog rescued tissue attachment and enhance callus formation. Together, our data demonstrate an ability for young tissues to facilitate grafting with distantly related species and identifies the PAT gene family as conserved regulators of graft healing and tissue regeneration.
Elucidating the role of muscarinic acetylcholine receptor (mAChR) signaling in efferent mediated responses of vestibular afferents in mammals
Authors: Sinha Anjali K. , Lee Choongheon , Holt Joseph C. 
Published: July 2023

AbstractThe peripheral vestibular system detects head position and movement through activation of vestibular hair cells (HCs) in vestibular end organs. HCs transmit this information to the CNS by way of primary vestibular afferent neurons. The CNS, in turn, modulates HCs and afferents via the efferent vestibular system (EVS) through activation of cholinergic signaling mechanisms. In mice, we previously demonstrated that activation of muscarinic acetylcholine receptors (mAChRs), during EVS stimulation, gives rise to a slow excitation that takes seconds to peak and tens of seconds to decay back to baseline. This slow excitation is mimicked by muscarine and ablated by the non-selective mAChR blockers scopolamine, atropine, and glycopyrrolate. While five distinct mAChRs (M1-M5) exist, the subtype(s) driving EVS-mediated slow excitation remain unidentified and details on how these mAChRs alter vestibular function is not well understood. The objective of this study is to characterize which mAChR subtypes drive the EVS-mediated slow excitation, and how their activation impacts vestibular physiology and behavior. In C57Bl/6J mice, M3mAChR antagonists were more potent at blocking slow excitation than M1mAChR antagonists, while M2/M4 blockers were ineffective. While unchanged in M2/M4mAChR double KO mice, EVS-mediated slow excitation in M3 mAChR-KO animals were reduced or absent in irregular afferents but appeared unchanged in regular afferents. In agreement, vestibular sensory-evoked potentials (VsEP), known to be predominantly generated from irregular afferents, were significantly less enhanced by mAChR activation in M3mAChR-KO mice compared to controls. Finally, M3mAChR-KO mice display distinct behavioral phenotypes in open field activity, and thermal profiles, and balance beam and forced swim test. M3mAChRs mediate efferent-mediated slow excitation in irregular afferents, while M1mAChRs may drive the same process in regular afferents.
Lakhpati Kisan programme: boosting the income of marginal farmers through women empowerment
Authors: Ajgaonkar Mihir , Mankodi Tanvi 
Published: July 2023

Learning outcomes
This case will help students to analyse and develop insights into the concepts of servant leadership; to analyse and develop insights into women’s empowerment and a process to achieve such empowerment; and to explore the social business models for scaling up.

Case overview/synopsis
The Lakhpati Kisan programme under the aegis of Tata Trusts focussed on empowering women marginal farmers in the tribal belts in India to significantly increase their income from 2015. Ganesh Neelam, Executive Director, Collectives for Integrated Livelihood Initiatives (CInI), a nodal agency of Tata Trusts, advocated various livelihood options in agriculture, livestock, non-timber forest resources and water conversation. Initially, CInI faced the challenge of getting the farmers to sign up for the programme due to lack of trust. CInI facilitators educated the farmers about the purpose of the initiative and the benefits they would accrue and built trust. CInI created awareness through knowledge-sharing sessions on best practices in agriculture. They formed self-help groups of farmers for decision-making and for easy access to capital. CInI established farmer producer organisations (FPOs) to bring in a business perspective among farmers. The farmers as Board members and executives ran the FPOs like commercial organisations. CInI built capabilities to create a sustainable and autonomous ecosystem that looked impressive. But still the programme was falling short of the desired target. The farmers were so far reluctant to move forward independently. Ganesh felt that the social business model that CInI had evolved needed a re-look to achieve a significant and lasting impact on the majority of the marginal farmers in India.

Complexity academic level
The case can be used in the organisation behaviour, human resource management courses and courses on social enterprises as part of the MBA or post-graduate management programme or in executive education programmes.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 6: Human Resource Management.
Italy and Spain at a crossroads: the politics of active social policies in southern Europe under a gender perspective
Authors: Giuliani Giovanni Amerigo , Rizza Roberto 
Published: July 2023

PurposeThe article explores to what extent party politics has influenced the different trajectories in Spain and Italy in terms of gendered active social policies (ASPs) (i.e. ALMPs and WLBPs). Second, it investigates how social and political modernization in the two countries has facilitated or hindered party competition on gendered ASPs.Design/methodology/approachTo investigate to what extent parties support gendered ASPs, the article relies on an original content analysis of party manifestos issued during the 2010s national elections. A total of 1387 quasi-sentences have been coded. The results were then quantified to graphically show how positions differentiate across parties and countries.FindingsThe content analysis of party manifestos displays that party politics matters: gendered ASPs are backed in a very different way by the Spanish and Italian parties. While in Spain all political parties have strongly championed ALMPs and WLBPs, this is not the case for the Italian parties. The research has also stressed that the specific path of social and political modernization is an important intervening variable that alters positively or negatively parties' support for gendered ASPs.Originality/valueThe article contributes to widen theoretically and empirically the literature on ASPs in the Southern European countries. Theoretically, it questions the supposed homogeneity of the Southern social model and investigated the alleged bifurcation between Italy and Spain, focusing on those policies – ASPs – that constitute the foundations of the Southern model: familialism and dualization. Furthermore, this bifurcation was analyzed adopting a gender perspective, and exploring adherence to or departure from the Southern model. Third, the article focuses on the politics of ASPs demonstrating that inspecting the political arena can contribute to explain policy change.
Gender diversity and collaboration with universities: drivers of innovation in family firms
Authors: Rodríguez-Gulías María Jesús , Rodeiro-Pazos David , Calvo Nuria , Fernández-López Sara 
Published: July 2023

PurposeThis paper provides empirical evidence for how gender diversity in top management teams (TMTs) and collaboration with university and technological centres lead to innovation outcomes. The authors review past research on these concepts and illustrate their individual and joint effects on process innovation specifically in the unique context of family firms (FFs).Design/methodology/approachThe authors used a sample of 788 Spanish manufacturing family firms in 2016 and applied logistic regression models since the dependent variables are dummies.FindingsThe authors found a positive relationship between gender-diverse TMTs, process innovation and research and development (R&D)-based process innovation. Similarly, the collaboration with university technological centres is positively associated with higher innovation outcome of FFs. In addition, the authors also found that the presence of women in TMTs shapes the relationship between the collaboration with university technological centres and process innovation.Originality/valueThis paper contributes to the research on collaborative innovation in FFs by emphasizing the collaboration with university technological centres, an external partner often ignored by this stream of literature. This research also responds to the calls for further study of the effect of the heterogeneity of the TMTs on the innovation outcome of FFs, from the perspective of the resource-based view (RBV) of the firms.
How and when social media influencers' intimate self-disclosure fosters purchase intentions: the roles of congruency and parasocial relationships
Authors: Koay Kian Yeik , Lim Weng Marc , Kaur Simran , Soh Kimberly , Poon Wai Ching 
Published: July 2023

PurposeThis study aims to explore the impact of social media influencers' (SMIs) intimate self-disclosure on consumers' purchase intentions, with a focus on the role of parasocial relationships and congruence among the influencer, product and consumer. The study finds its theoretical foundation in parasocial theory and self-congruency theory.Design/methodology/approachThis study employs a quantitative approach, utilizing a sample of 232 collected survey responses. The proposed hypotheses are evaluated using partial least squares structural equation modeling (PLS-SEM).FindingsContrary to initial assumptions, the study reveals that parasocial relationships do not mediate the link between SMIs' intimate self-disclosure and purchase intentions. However, an interesting moderating effect was discovered: the congruence between the consumer and the influencer influences the relationship between SMIs' intimate self-disclosure and parasocial relationships, and subsequently, between parasocial relationships and purchase intentions.Originality/valueThis study brings fresh insights by pinpointing a boundary condition that dictates the influence of SMIs' intimate self-disclosure on purchase intentions via parasocial relationships. In doing so, this study provides a novel perspective in understanding the dynamics between SMIs and consumers in the rapidly evolving marketing landscape.
The role of letter knowledge acquisition ability on children's decoding and word identification: Evidence from an artificial orthography
Authors: Acha Joana , Rodriguez Nuria , Perea Manuel 
Published: July 2023

BackgroundLetter knowledge is crucial in the first stages of reading development. It supports learning letter‐sound mappings and the identification of the letters that make up words. Previous studies have investigated the longitudinal impact of early letter knowledge on children's further word reading abilities. This study employed an artificial orthography learning paradigm to explore whether the rate of letter learning modulates children's reading and word identification skills.MethodsIn an initial training phase, 8‐year‐old Spanish children (N = 30) learned nine artificial letters and their corresponding sounds (two vowels and six consonants). The letter learning rate was set according to the number of attempts needed to name at least seven letters (i.e., 80% correct). These ranged from 1 to 4. In a second training phase, children visualized words made up of the trained letters while listening to their pronunciations. Some words included a context‐dependent syllable (i.e., leading to grapheme‐to‐phoneme inconsistency), and others had an inconsistent syllable (i.e., phoneme‐to‐grapheme inconsistency). The post‐test consisted of a reading aloud task and an orthographic‐choice task in which the target word was presented with a distractor equal to the target except for the substitution of a letter.ResultsChildren showed a high accuracy rate in the post‐test tasks, regardless of whether words contained context‐dependent or inconsistent syllables. Critically, the letter learning rate predicted both reading aloud and identification accuracy of words in the artificial orthography.ConclusionsWe provide evidence for the vital role of letter knowledge acquisition ability in children's decoding and word identification skills. Training children on this ability facilitates serial letter‐sound mapping and word identification skills. Artificial orthography paradigms are optimal for exploring children's potential to achieve specific literacy skills.
Inequalities and content moderation
Authors: De Gregorio Giovanni , Stremlau Nicole 
Published: July 2023

AbstractAs the harms of hate speech, mis/disinformation and incitement to violence on social media have become increasingly apparent, calls for regulation have accelerated. Most of these debates have centred around the needs and concerns of large markets such as the EU and the United States, or the aggressive approach countries such as Russia and China adopt to regulate online content. Our focus in this article is with the rest, the smaller markets at the periphery of the advertising industry, and the deep inequalities that current approaches to content moderation perpetuate. We outline the depth of the unequal practice of moderation, particularly across Africa, and explore the underlying political and economic factors driving this gap. While recognizing content moderation has many limitations, we conclude by underlining potential approaches to increase oversight in content moderation.
Identification of predictive factors for early relapse in patients with unresectable stage III non‐small cell lung cancer receiving consolidation durvalumab after concurrent chemoradiation therapy
Authors: Nam Jung Hyun , Yeo Chang Dong , Park Chan Kwon , Kim Sung Kyoung , Kim Ju Sang , Kim Yong Hyun , Kim Jin Woo , Kim Seung Joon , Lee Sang Haak , Kang Hye Seon 
Published: July 2023

AbstractBackgroundPatients with locally advanced, unresectable, non‐small cell lung cancer (NSCLC) receiving definitive concurrent chemoradiation therapy (CCRT) benefit from durvalumab consolidation therapy. However, predictive factors for early relapse during durvalumab maintenance have not yet been identified.MethodsThe present study included the lung cancer cohort of the Catholic Medical Centers at the Catholic University of Korea from January 2018 to December 2021. A total of 51 NSCLC patients treated with durvalumab consolidation therapy after definitive CCRT were included in the analysis. Early relapse was defined as patients experiencing relapse within 6 months of starting initial durvalumab therapy.ResultsAmong the 51 patients, 15 (29.4%) relapsed during the study period. Median time from initial therapy of durvalumab to progression was 451.00 ± 220.87 days (95% confidence interval [CI]: 18.10–883.90) in overall patients. In multivariate analysis, younger age (adjusted odds ratio [aOR], 0.792; 95% CI: 0.642–0.977; p = 0.030), higher pack‐years (aOR, 1.315; 95% CI: 1.058–1.635; p = 0.014), non‐COPD (aOR, 0.004; 95% CI: 0.000–0.828; p = 0.004) and anemia (aOR, 234.30; 95% CI: 1.212–45280.24; p = 0.042), were independent predictive factors for early relapse during durvalumab consolidation therapy.ConclusionYounger age, higher number of pack‐years, non‐COPD, and anemia were independent predictive factors for early relapse during durvalumab consolidation therapy in patients with unresectable stage III NSCLC after definitive CCRT. Careful patient selection and clinical attention are needed for high‐risk individuals.
Water conditions influence the detectability of Crossodactylus gaudichaudii (Anura, Hylodidae) in streams of the Atlantic Forest
Authors: Pereira‐Ribeiro Juliane , Linause Thais , Ferreguetti Átilla C. , Cozer Jonathan , Bergallo Helena G. , Rocha Carlos Frederico D. 
Published: July 2023

AbstractStream‐dwelling amphibians' occurrence, behaviour and reproductive success are strongly influenced by dynamic abiotic factors, for example, water flow and spray. These factors can disproportionately affect these frogs due to their dependency on specific favourable conditions for development and incapacity to disperse from unfavourable environments. We analysed the influence of environmental covariates on the detectability of Crossodactylus gaudichaudii, an amphibian species endemic to streams in the Brazilian Atlantic Forest. We conducted sampling in the streams of the Duas Bocas Biological Reserve, Brazil, and we measured air and water temperature, air humidity and pH of water on each sampling occasion. We estimated the effects of variables on the detectability of the species using single‐season occupancy models. Our results indicated that the detectability of the species increases on occasions with higher water temperatures (24–26°C) and lower pH (5.0–5.5). We investigated the influence of these covariates only on the detectability of adult frogs, but it is likely that the physiochemical properties of stream water are more important to aquatic larvae, and hence, the probability of their detection. Given this, further studies should examine the relevance of covariates on the detectability of adult frogs as well as larvae.
Risk factors of portal vein system thrombosis after splenectomy: a meta‐analysis
Authors: Lin Yu‐Kai , Cai Xin‐Ran , Hong Hai‐Jie , Chen Jiang‐Zhi , Chen Yan‐Ling , Du Qiang 
Published: July 2023

AbstractBackgroundThe primary aim of the present study was to explore risk factors for portal vein system thrombosis following splenectomy.MethodsA systematic search of PubMed, Embase and Cochrane libraries was conducted to identify original studies that fulfilled the inclusion criteria. Raw data on potential risk factors for portal vein system thrombosis after splenectomy were extracted for meta‐analysis. Subsequently, a sensitivity analysis was conducted to verify the stability of the results.ResultsEighteen studies with 626 thrombosis events from 1807 splenectomy met the inclusion criteria. Larger spleen volume (SMD 0.44, P = 0.000), broader splenic vein diameter (WMD 2.30, P = 0.000), broader portal vein diameter (WMD 2.08, P = 0.000), a lower velocity of portal blood flow (WMD −0.91, P = 0.001), decreased platelet count (WMD −5.14, P = 0.007), decreased white blood cell (WMD −0.40, P = 0.027), decreased haemoglobin (WMD −9.14, P = 0.002), ascites (OR 1.81, P = 0.003) and bleeding history before surgery (OR 1.88, P = 0.002) were identified to be factors that exacerbated the risk of portal vein system thrombosis after splenectomy. Sex, age, preoperative prothrombin time, postoperative platelet count, postoperative D‐dimer, operation time and intraoperative blood loss, did not increase the risk of thrombosis.ConclusionLarger spleen volume, broader splenic vein diameter, broader portal vein diameter, a lower velocity of portal blood flow, ascites, bleeding history before surgery, decreased platelet count, white blood cell and haemoglobin may increase the risk of portal vein system thrombosis.