bluuebunny/crossref_metadata_2025_split huggingface dataset:
Análisis del escaso relevo generacional desde los modelos de desarrollo rural en Colombia
Authors: Rodríguez Espinosa Uriel , Correa Perdomo Andrea Victoria 
Published: February 2023

Contextualización: el escaso relevo generacional en el ámbito productivo rural es una problemática en constante crecimiento a nivel global, este fenómeno afecta, principalmente, la agricultura a pequeña escala.
Vacío de conocimiento: los modelos de desarrollo rural implementados por los Estados están inmersos en modelos de desarrollo y teorías que a su vez responden a paradigmas de desarrollo asimilados por los diferentes gobiernos. En la actualidad se carece de análisis que relacionen la incidencia de tales modelos de desarrollo en problemáticas como el escaso relevo generacional rural.
Propósito: describir como han incidido los diferentes modelos de desarrollo rural implementados en Colombia en la problemática de escaso relevo generacional de la agricultura a pequeña escala.
Metodología: se llevó a cabo una revisión descriptiva de literatura gris, enfatizando en textos históricos del desarrollo rural en Latinoamérica y Colombia.
Resultados y conclusiones: se encontró que la implementación de modelos de desarrollo rural que desconocen la importancia de la agricultura a pequeña escala y que por el contrario sólo se han interesado en la agricultura comercial moderna ha generado el atraso, pobreza y marginación de la pequeña agricultura, y en consecuencia han forzado la migración de personas, principalmente las más jóvenes, del campo a la ciudad que salen en búsqueda de mejores condiciones laborales, seguridad, educación y mejores condiciones de vida. Se concluye que entre las principales teorías del desarrollo que han incidido en la problemática se encuentran la teoría de la modernización, la estructuralista y la neoliberal.
Antibody Response to SARS-CoV-2 Infection in Asymptomatic Bangladeshi Female Garment Workers
Authors: Nur Jasmin , Alam Md. Sohrab , Belal Md. Hasan , Rahman Zeenat Farzana , Moniruzzaman Mohammad , Begum Tanjima , Khan Mansura , Hassan M. Sawkat 
Published: February 2023

Antibody titer study is very important to assess the degree of undetected transmission in the community. This study aimed to estimate the antibody response to SARS-CoV-2 infection in a group of asymptomatic female workers and compare the clinical and socio-demographic parameters of seropositive and seronegative subjects in Bangladesh. This cross-sectional descriptive study was conducted among 285 female workers who had come to BIRDEM General Hospital for the COVID-19 test in November 2021 from different areas of Bangladesh. RBD-specific antibody responses against SARS-CoV-2 were measured by using Chemiluminescent Microparticle Immunoassay (CMIA) for all participants. Overall antibody positivity was estimated at 91.9% (262), and only 8.1% of participants had a negative IgG antibody response. Of the 262 participants 96.18% were asymptomatic. In the age group, the highest seropositivity was between 21-30 years (58%). The median IgG titer of the antibody was estimated at 578.50 AU/ml. The participants of Khulna had the highest median IgG titer of 3966 AU/ml followed by other areas (p=0.043). There was no significant difference in median IgG titer in symptomatic, asymptomatic, and vaccinated, non-vaccinated participants. Based on the geographical area Dhaka and Barisal had significantly more seropositive subjects than seronegative subjects (p = 0.001) and (p = 0.011). The high number of undiagnosed COVID-19 patients in the population is reflected in the percentage of seropositive people. Our findings will assist academics in better understanding immune status and policymakers in taking proactive measures during pandemic situations and future health emergencies.
HUBUNGAN KEBIASAAN SARAPAN PAGI DAN KEBIASAAN JAJAN DENGAN STATUS GIZI ANAK SD NEGERI 026602 KOTA BINJAI
Authors: Hutabarat Suherlina 
Published: February 2023

ABSTRAKAnak usia sekolah dasar merupakan salah satu kelompok yang rawan mengalami masalah gizi, maka dari itu di butuhkan perhatian khusus agar anak terhidar dari masalah gizi. Sarapan pagi salah satu sumber masuknya zat gizi yang baik untuk memenuhi asupan harian namun masih banyak anak yang tidak biasa sarapan pagi. Selain itu tingginya kebiasaan jajan anak juga dapat mempengaruhi status gizi anak karena umumnya jajanan yang di jual tinggi gula, tinggi lemak dan tinggi energi.Penelitian ini bertujuan untuk menganalisis hubungan kebiasaan sarapan pagi dan kebiasaan jajan dengan status gizi anak SD. Desain penelitian cross sectional. Lokasi penelitian yaitu di SD Negeri 026602 Kota Binjai. Waktu penelitian Mei 2022. Teknik pengambilan sampel sampel dilakukan secara total sampling sehingga jumlah sampel adalah 50 orang. Teknik pengumpulan data kebiasaan sarapan dan kebiasaan jajan diambil menggunakan skor interval yang diperoleh dari kuesioner, frekuensi konsumsi jenis menu sarapan pagi dan jajan diambil menggunkan skor rata-rata FFQ selama 1 minggu dan data status gizi di ambil menggunakan hasil pengukuran antropometri yaitu tinggi badan dan berat badan. Teknik analisis data yang digunakan adalah deskripsi data, uji korelasi Rank Spearman dan uji regresi linear berganda menggunakan uji F. Berdasarkan hasil uji korelasi rank spearman didapatkan hasil adanya hubungan yang positif dan signifikan antara kebiasaan sarapan pagi dengan status gizi dengan nilai korelasi 0,492 dan adanya hubungan yang positif dan signifikan antara kebiasaan jajan dengan status gizi dengan nilai korelasi 0,415. Berdasarkan hasil uji regresi linear berganda dengan uji F, adanya hubungan yang positif dan siginifikan antara kebiasaan sarapan pagi dan kebiasaan jajan dengan status gizi anak SD Negeri 026602 Kota Binjai. (Y = 1,341 + 0,033x1 + 0017x2 ). Kesimpulannya terdapat hubungan yang positif dan signifikan antara kebiasaan sarapan pagi dan kebiasaan jajan dengan status gizi pada anak SD Negeri 026602 Kota Binjai. Kata Kunci : Kebiasaan sarapan pagi, Kebiasaan jajan, Status gizi.
Matrix metalloproteinase-2 (MMP-2 ) and-9 (MMP-9) gene variants and microvascular complications in type 2 diabetes patients
Authors: Andjelic Jelic M , Radojkovic D , Nikolic A , Rakicevic Lj , Babic T , Jelic D , Lalic NM 
Published: February 2023

Abstract
Vascular complications are the leading cause of increased morbidity and mortality of diabetic patients. It has been postulated that matrix metalloproteinases MMP-2 and MMP-9, zinc-dependent endopeptidases through remodeling of the extracellular matrix, can contribute to the onset and progression of diabetic vascular complications. The aim of our study was to assess whether there is a major difference in single nucleotide polymorphisms in the MMP-2 (at position -1306C˃T) and MMP-9 (at position -1562C˃T) gene in type 2 diabetic patients and healthy controls and to determine whether there is an association of these gene variants with the presence of microvascular complications in diabetic patients. Our study included 102 type 2 diabetes patients and a control group which was comprised of 56 healthy controls. All diabetic patients were screened for microvascular diabetes complications. Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies were determined. The MMP-2 variant -1306C>T showed a negative correlation with type 2 diabetes (p=0.028). It was also shown that the presence of the -1306C allele increases the probability of developing type 2 diabetes. This was a 2.2 fold increase and that the -1306 T allele has a protective role in regards to type 2 diabetes. The MMP-2 variant -1306T showed a negative correlation with diabetic polyneuropathy (p=0.017), meaning that allele-1306T has a protective role in regards to diabetic polyneuropathy while the presence of allele -1306C increases the probability of developing diabetic polyneuropathy by 3.4 fold. Our study showed that the MMP-2 gene variant (-1306C) doubles the risk of developing type 2 diabetes, and for the first time an association of this gene variant and the presence of diabetic polyneuropathy was shown.
A novel Loss-of-function Mutation in MYBPC3 Causes familial hypertrophic cardiomyopathy with extreme intrafamilial phenotypic heterogeneity
Authors: Peng Y , Xu J , Wang Y , Zhao J , Zhang L , Chen Z , Jiang Y , Banerjee S , Zhang Z , Bai M 
Published: February 2023

Abstract
Cardiomyopathies are a heterogeneous group of diseases predominantly affecting the heart muscle and often lead to progressive heart failure-related disability or cardiovascular death. Hypertrophic cardiomyopathy (HCM) is a cardiac muscle disorder mostly caused by the mutations in genes encoding cardiac sarcomere. Germ-line mutations in MYBPC3 causes hypertrophic cardiomyopathy (HCM). However, most of the HCM associated MYBPC3 mutations were truncating mutations. Extreme phenotypic heterogeneity was observed among HCM patients with MYBPC3 mutations. In this study, we investigated a Chinese man who presented with HCM. Whole exome sequencing identified a novel heterozygous deletion (c.3781_3785delGAGGC) in exon 33 of the MYBPC3 in the proband. This heterozygous variant causes frameshift (p.Glu1261Thrfs*3), which predicted to form a truncated MYBPC3 protein. The proband’s father also carries this variant in a heterozygous state while the proband’s mother did not harbor this variant. Here, we report on a novel deletion in the MYBPC3 gene associated with HCM. We also highlight the importance of whole exome sequencing for molecular diagnosis for the patients with familial HCM.
Single nucleotide polymorphisms in IL-1A RS1800587, IL-1B RS1143634 and vitamin D receptor rs731236 in stage III grade B/C periodontitis
Authors: Özturk Özener H. , Tacal Aslan B. , Eken B.F. , Agrali Ö.B. , Yildrim H.S. , Altunok E.Ç. , Ulucan K. , Kuru L. 
Published: February 2023

Abstract
The purpose of the study is to determine the prevalence of interleukin (IL)-1A (rs1800587), IL-1B (rs1143634) and vitamin D receptor (VDR) (TaqI, rs731236) gene polymorphisms in the Turkish population and their association with Stage III Grade B/C periodontitis. Systemically and periodontally healthy individuals (N = 100) and Stage III Grade B/C periodontitis patients (N=100) based on clinical and radiographic examination were included in this research. Clinical attachment level, probing depth, bleeding on probing, plaque and gingival indices of the subjects were measured. Genotyping of IL-1A (rs1800587), IL-1B (rs1143634) and VDR (rs731236) polymorphisms was conducted by Real Time PCR. Allelic and genotypic distributions of IL-1A (rs1800587) gene polymorphism were not associated with periodontitis (p>0.05). In IL-1B (rs1143634) gene polymorphism, the C allele was detected more frequently in healthy individuals compared with the periodontitis patients (p=0.045). CC genotype and C allele in VDR (rs731236) gene polymorphism was higher in periodontitis patients (p=0.031, p=0.034, respectively). In comparison with Grade B periodontitis patients and healthy subjects, CC genotype and C allele were observed more frequently in the Grade B periodontitis in terms of alleles (C/T) and genotypes for VDR (rs731236) polymorphism (p=0.024, p=0.008, respectively). This study presents that the VDR (rs731236) polymorphism are associated with enhanced susceptibility to Stage III periodontitis in the Turkish population. Furthermore, VDR (rs731236) polymorphism may be used as an identification criteria to discriminate Grade B and Grade C in Stage III periodontitis.
A novel likely pathogenic variant in the RUNX1 gene as the cause of congenital thrombocytopenia
Authors: Despotović M , Pereza N , Peterlin B , Ostojić S , Golob B , Maver A , Roganović J 
Published: February 2023

Abstract

Introduction
Heterozygous pathogenic and likely pathogenic sequence variants in the RUNX1 (Runt-related Transcription Factor 1) gene are a common genetic cause of decreased platelet count and/or platelet dysfunction and an increased risk of developing myelodysplasia and acute myeloid leukemia. The majority of causative variants are substitutions, which rarely occur de novo. The aim of this case report is to present a patient with congenital thrombocytopenia caused by a deletion variant in exon 9 in the RUNX1 gene.

Case report
A one-month-old male infant was admitted to the Clinical Hospital Center Rijeka because of anemia and thrombocytopenia verified in the course of an acute viral infection. During follow-up, he occasionally had petechiae and ecchymoses on the lower extremities after mild trauma, with no other symptoms. The patient had persistent slightly decreased values of platelets with normal morphology, but with pathological aggregation with adrenaline and adenosine diphosphate. Due to the unclear etiology of persistent mild thrombocytopenia, he was referred for genetic testing at the age of five. Genomic DNA was isolated from the patient’s peripheral blood and whole-exome sequencing was performed using the next-generation sequencing method. A heterozygous frameshift variant, c.1160delG (NM_001754.4), was identified in exon 9. The variant is classified as likely pathogenic.

Conclusion
To the best of our knowledge, the heterozygous variant c.1160delG in the RUNX1 gene was first described in our patient. Although pathogenic variants in the RUNX1 genes are very rare, persistently low platelet counts of unclear etiology should raise suspicion of an underlying genetic disorder.
A novel variant in the LIPA gene associated with distinct phenotype
Authors: Sarajlija A. , Armengol L. , Maver A. , Kitic I. , Prokic D. , Cehic M. , Djuricic M.S. , Peterlin B. 
Published: February 2023

Abstract
Deficiency of lysosomal acid lipase (LAL-D) is caused by biallelic pathogenic variants in the LIPA gene. Spectrum of LAL-D ranges from early onset of hepatosplenomegaly and psychomotor regression (Wolman disease) to a more chronic course (cholesteryl ester storage disease - CESD). The diagnosis is based on lipid and biomarker profiles, specific liver histopathology, enzyme deficiency, and identification of causative genetic variants. Biomarker findings are a useful for diagnostics of LAL-D, including high plasma concentration of chitotriosidase as well as elevated oxysterols. Current treatment options include enzyme replacement therapy (sebelipase-alpha), statins, liver transplantation, and stem cell transplantation.
We present two pairs of siblings from Serbia with a distinctive phenotype resembling LAL-D with a novel variant of unknown significance (VUS) detected in the LIPA gene and residual LAL activity. All patients presented with hepatosplenomegaly at early childhood. In siblings from family 1, compound heterozygosity for a pathogenic c.419G>A (p.Trp140Ter) variant and a novel VUS c.851C>T (p.Ser284Phe) was detected. Patients from family 2 were homozygous for c.851C>T VUS and both have typical histopathologic findings for LAL-D in the liver. Enzyme activity of LAL was tested in three patients and reported as sufficient, and therefore enzyme replacement therapy could not be approved.
When confronted with a challenge of diagnosing an inherited metabolic disorder, several aspects are taken into consideration: clinical manifestations, specific biomarkers, enzyme assay results, and molecular genetic findings. This report brings cases to light which have a considerable discrepancy between those aspects, namely the preserved LAL enzyme activity in presence of clinical manifestations and rare variants in the LIPA gene.
APOE4 Status and cognitive function in middle-aged and elderly people
Authors: Pavel N.A. , Paun M.R. , Matei P.V. , Dutu I. , Tudose C. 
Published: February 2023

Abstract

Introduction

APOE is one of the prominent genes involved in the increased risk of developing Alzheimer’s disease, but its effect on cognition in patients who are not yet diagnosed with dementia or mild cognitive impairment is relatively understudied. We aimed to examine the effect of ApoE4 on cognitive performance in unimpaired middle-aged and elderly persons.

Materials and methods
Our study included 51 cognitively unimpaired participants divided into ApoE4 positive patients and controls by APOE genotyping. The following clinical and demographic characteristics were collected: age, gender, education, social status, BMI, history of medical or psychiatric disorders. Patients with current anxiety or depressive disorders were excluded. Cognitive function was evaluated using MMSE, Rey Auditory-Verbal Learning Test, Rey Complex Figure test, TMT A and B and verbal fluency test. The two groups were matched for age, sex, and education. Categorial data was analyzed using Chi-Square and continuous data using Student-T test (parametric variables) or Mann-Whitney test (non-parametric variables). Statistical significance was considered at p≤.05.

Results
There were 11 (21.6%) ApoE4 positive patients and 40 (78.4%) controls. There were no significant differences between the groups regarding socio-demographic and clinical characteristics. The ApoE4 positive group performed slightly worse on cognitive evaluations compared to controls but only the mean scores of the Rey Complex Figure Test – Memory reached statistical significance (p=.019).

Conclusion
Cognitive evaluation generally rendered lower scores in the ApoE4 group compared to the control group. However, only visual memory impairment scores were significantly lower in the ApoE4 positive individuals than in controls.
PELATIHAN STIMULASI KETERAMPILAN LITERASI AWAL ANAK USIA DINI MELALUI MEDIA POSTER DI MASA PANDEMI
Authors: Baiti Noor , Zulkarnaen Muhammad 
Published: February 2023

Kemampuan bahasa berkaitan erat dengan kemampuan literasi anak. Literasi dapat dikategorikan dalam berbagai bidang kemampuan diantaranya ialah kemampuan membaca, menulis dan memahami. Pemahaman terhadap menjaga kebersihan lingkungan sekitar anak dan menggunakan protokol kesehatan selama berada di luar rumah sangat perlu dilakukan. Penanaman pemahaman terhadap anak dapat dilakukan dengan berupa stimulasi literasi yang diberikan kepada anak salah satunya dapat berupa poster. Terutama dalam meningkatkan pemahaman anak terhadap gejala dan pencegahan virus covid-19. Kegiatan pengabdian kepada masyarakat dilaksanakan dengan menggunakan cara tatap muka, metode ceramah dan demonstrasi serta pendampingan dalam praktek. Pelatihan ini bertujuan untuk menstimulasi keterampilan literasi awal anak menggunakan media poster. Hasil kegiatan pelatihan ini diharapkan dapat membantu stimulasi keterampilan literasi awal anak menggunakan media poster di Desa Sungai Punggu Baru Kecamatan Anjir Muara Kabupaten Barito Kuala.
PELATIHAN SURFING TINGKAT DASAR BERBASIS PERMAINAN UNTUK ANAK PESISIR BARAT LAMPUNG
Authors: Siregar Nofi Marlina , Fitri Novita Sari Eka , Budiningsih Marlinda , Suryani Lilis 
Published: February 2023

Pengabdian ini bertujuan untuk membuat Pelatihan Surfing Tingkat Dasar Berbasis Permainan Untuk Anak Pesisir Barat Lampung. Dengan tujuan pengabdian untuk mengembangkan keterampilan bermain surfing seperti menggayuh papan, berdiri dipapan, dan bermain dilaut Pengabdian ini menggunakan metode pengabdian berupa Pratek dan Ceramah, dimana narasumber meberikan materi teori dan pratek tentang surfing kepada peserta pelatihan surfing. Pelatihan ini dilakukan oleh 2 orang narasumber, 8 orang tim pendukung, dilaksanakan di Pesisir Pantai Krui yang berada di wilayah Labuhan Jukung Krui Pesisir Barat Lampung dengan subjek pengabdian sebanyak 30 anak yang berada di sekiitar Pesisir Pantai Krui Pesisir Barat Lampung. Dari hasil pengamatan saat pelatihan ini, anak anak peserta pelatihan memiliki semangat dan motivasi besar dari awal sampai akhir pelatihan. peserta sangat puas dan senang dengan adanya pelatihan seperti ini, karena sebelumnya tidak ada pelatihan surfing terhadap mereka, mereka hanya melihat lihat saja ketika ada masyarakat lokal maupun para turis mancanegara yang sedang bermain surfing. Berdasarkan hasil pengabdian diatas dapat disimpulkan bahwa Motivasi dan antusiasme anak anak dalam mengikuti Pelatihan ini sangat baik.bahkan orang tua dan masyarakat setempat sangat mendukung diadakan pelatihan ini. Terjadi interaksi yang aktif antara tim pelaksana dengan pengurus surfing, pelatih dan peserta pelatihan ini. Bertambahnya pengetahuan dan pemahanan dalam keterampilan bermain surfing. Bertambahnya wawasan tentang laut, ombak dan lain lain. Bertambahnya wawasan dan pengetahuan tentang model latihan surfing dari yang ringan sampai yang berat. Pelatihan surfing tingkat pemula sangat lah penting agar bisa mengenalkan olahraga surfing pada anak-anak, sehingga mereka termotivasi dan tujuan memasyarakat kan olahraga dan mengolahragakan masyarakat dapat terwujud.
Anagram Game to Teach Vocabulary Mastery for Islamic Junior High School Students
Authors: Mutiah Wardah , Harida Eka Sustri , Oktapian Ardi 
Published: February 2023

This research has investigated the students’ vocabulary mastery using Anagram for the Islamic Junior High School students in Padangsidimpuan. It was done through experimental method with pre-test and post-test design, with multiple choice test. There was 39 students as experimental class as the sample of this research, and mean-score was used to analysis the data in this research. After getting the data, the mean score of pre-test result is 51.72 and after using Anagram became 62.09. It can be said that anagram game has given good effect to the students’ vocabulary mastery of the VII grade students of the Islamic Junior high school students.
Assessment of Groundwater Suitability for Irrigation and Livestock Purposes in Sayed Al-Shuhadaa Agricultural City, Karbala, Central Iraq
Authors: Mahmoud Rafal Ghassan , Al Shamma'a Ayser , Al-Ghanimy Maithem 
Published: February 2023

The study investigates the anion and cation concentrations and their distribution in the Dammam aquifer to assess the groundwater suitability for irrigation and livestock in Sayed Al-Shuhadaa, Karbala, central Iraq. It lies between longitudes 43º 29' 00'' – 43º 40' 00'' E and latitudes (32º 17' 00'' – 32º22' 00'') N. The physicochemical properties, cations and anions were measured in 14 active wells distributed in the study area. The assessment was conducted based on the sodium adsorption rate (SAR), sodium percentage (Na%), electrical conductivity (EC), total dissolved solids (TDS), and Hydrogen number (pH). Groundwater in the study area is very good for livestock and characterised by no damage, as the rate of sodium adsorption was good. The percentage of sodium and pH values are within the permissible values for irrigation, except for some wells that have high TDS and EC, which leads to an increase in salinity.
Extended Eigenvalues and Eigenoperators of Some Weighted Shift Operators
Authors: Hussein Kareem M. , Ahmed Buthainah A. 
Published: February 2023

A complex number is called an extended eigenvalue for an operator on a Hilbert space H if there exists a nonzero operator such that: such is called an extended eigenoperator corresponding to. The goal of this paper is to calculate extended eigenvalues and extended eigenoperators for the weighted unilateral (Forward and Backward) shift operators. We also find an extended eigenvalues for weighted bilateral shift operator. Moreover, the closedness of extended eigenvalues for the weighted unilateral (Forward and Backward) shift operators under multiplication is proven.