bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐Cell RNA Sequencing Reveals the Developmental Landscape of Wheat Roots
Authors: Du Zhenzhen , Zhang Bin , Weng Han , Gao Li 
Published: January 2025

ABSTRACTAllohexaploid wheat (Triticum aestivum L.) is one of the major crops worldwide, however there is very limited research on the transcriptional programmes of underlying cell type specification. Single‐cell RNA sequencing (scRNA‐seq) was used to unravel the transcriptome heterogeneity of cells and the composition of cell types in broad‐spectrum organisms. Here, we reported the scRNA‐seq transcriptomes of single cells from root tips of the wheat Chinese spring (CS) cultivar, defined cell‐type‐specific marker genes, and identified most of the major cell types. We further profiled the reconstructed developmental trajectories of the stem cell niche (SCN), proximal meristems and meristems, unveiled gene expression signature of water transportation, divulged cell‐type‐specific asymmetric gene transcription in subgenomes and explored the evolutionary conservation and divergence of wheat cultivar (CS) and rice cultivar (Nip and 93‐11, ZH11) cell types through interspecies comparison. Collectively, this work underscored the transcriptional landscape of wheat cultivar (CS) roots and provided a single‐cell perspective for differentiation trajectory application, unbalanced gene expression pattern and characteristics of cell types between two plant species, contributing to a better understanding of wheat cultivar (CS) root development at unprecedented resolution.
Effect of Brochure and Audio Recordings of Testicular Cancer Stories on Testicular Self‐Examination: Randomized Controlled Study
Authors: Ucar Ayse Kılıc , Aykac Irem , Tastan Sevinc 
Published: January 2025

ABSTRACTObjectivesThe aim of this study was to determine the effects of a training program based on educational brochures and audio recordings of testicular cancer (TC) stories on testicular self‐examination (TSE) and health beliefs among university students.DesignThe study had a pretest–posttest, control group experimental design.SampleThis study was conducted with 106 students in Turkey.MethodData were collected using a personal information form, the Health Beliefs Model (HBM) Scale for TC and TSE, and a form for determining TSE and sharing information.ResultsThere was a statistically significant difference between the posttest scores obtained by the intervention group, who received the training and the control group on the susceptibility, benefits and health motivation, barriers, self‐efficacy, and health motivation dimensions of the HBM Scale (p < 0.05). The percentage of TSE in the intervention group increased from 14.8% to 90.7% after receiving training on TC and TSE, which was significantly different from the pretest and posttest scores of the control group.ConclusionsTraining using educational brochures and audio recordings of TC stories had a positive effect on TSE and health beliefs. The results suggest that both training methods can be used to increase social awareness of TC.
Timing of Delivery of Low‐Risk Persons and the Risk of Attention‐Deficit Hyperactivity Disorder in Offspring: Sweden and British Columbia, Canada
Authors: Nguyen Thi Hoang Ha , Hossin M. Zakir , Schmauder Stefanie , Muraca Giulia M. , Lisonkova Sarka , Razaz Neda 
Published: January 2025

ABSTRACTBackgroundAn evidence gap exists concerning the timing of delivery at 37–42 weeks and the risk of attention‐deficit hyperactivity disorder (ADHD) in offspring.ObjectiveTo determine the association between timing of delivery in low‐risk pregnancies at term (37–42 weeks) gestations and ADHD in offspring.MethodsThis population‐based cohort study comprised 1,424,453 singletons in Sweden and 403,765 in British Columbia (BC), Canada, live‐born at 37–42 completed weeks to low‐risk pregnant women between 2000 and 2018. Children were followed up from age 1 until the date of death, emigration, their first diagnosis, or December 2020 (study's end date). The exposure was time of delivery assessed through gestational age, and the outcome was the diagnosis of ADHD. Cox regression models were used to examine the association between gestational age at delivery and ADHD.ResultsDuring the follow‐up period, 59,989 children in Sweden were diagnosed with ADHD (4.5 per 1000 child‐years). Correspondingly, in BC, during the same period, there were 27,445 children diagnosed with ADHD (7.4 per 1000 child‐years). In Sweden, the adjusted hazard of ADHD was 10%, 6%, and 3% higher at 37, 38, and 39 weeks gestation compared with those born at ≥ 38, ≥ 39, and ≥ 40 weeks, respectively. In BC, the corresponding hazards were 9%, 6%, and 3%, respectively. Both regions showed no elevated ADHD risks for infants born at 40 weeks compared to those born at ≥ 41 weeks, with slightly lower rates at 40 weeks.ConclusionsIn low‐risk pregnancies, births at 37 and 38 weeks were associated with a higher ADHD risk, while births at 40 weeks showed no increased risk compared with those born at later gestations.
Breaking down barriers: Improving accessibility and inclusivity for people with disabilities in veterinary ophthalmology
Authors: O'Leary Louise 
Published: January 2025

AbstractOver 25% of people in the United States are affected by physical disabilities, leading to regular interactions between the disabled community and veterinary ophthalmology. However, various barriers hinder individuals with disabilities from accessing care for their pets, working in veterinary clinics, pursuing training as veterinarians and veterinary ophthalmologists, and continuing their careers as disabilities develop. This article explores strategies for the veterinary ophthalmology community to implement positive changes that enhance access and inclusivity within clinics and the profession at practical, institutional, and cultural levels.
Effectiveness of cognitive behavioral therapy vs conservative pain management for occupational performance in patients with low back pain: a systematic review protocol
Authors: Delasas Gemiele Elaine , Delgado Ninfa Mae , Mojica Isabelle Kezia , Ragasa Stepanie , Dones Valentin , Manlapaz Donald 
Published: January 2025

Objective:
This review aims to evaluate the effectiveness of cognitive behavioral therapy (CBT) compared with conservative pain management for improving the occupational performance of individuals with low back pain.

Introduction:
Low back pain is a condition that describes pain between the lower edge of ribs and the buttocks. It is projected to affect 843 million people by 2050, with significant economic and occupational consequences. CBT is a growing treatment for chronic pain that aims to change maladaptive thoughts and behaviors. This review will examine the effectiveness of CBT for improving occupational performance.

Inclusion criteria:
The review will focus on the effect of CBT on adults with musculoskeletal low back pain, whether acute, subacute, or chronic. It will analyze randomized controlled trials, cohort studies, case-control studies, and quasi-experimental studies to assess CBT’s impact on occupational performance, including activities of daily living, work, and sleep.

Methods:
Two independent reviewers will screen titles, abstracts, and full-text manuscripts from PubMed, CINAHL (EBSCOhost), Web of Science Core Collection, Cochrane Library, OTSeeker, and ScienceDirect. Unpublished studies and gray literature will also be searched in ClinicalTrials.gov, Google Scholar, World Health Organization, and ProQuest Dissertations and Theses. A subgroup analysis will explore the effects of CBT on post-treatment outcomes, identifying sources of heterogeneity in its impact on activities of daily living, work, and sleep. Factors such as CBT type, delivery method, participant age, and low back pain chronicity will be considered. The Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach will assess evidence certainty, and a Summary of Findings will be provided.

Registration number:
PROSPERO CRD42023472522
The literature exploring the perspectives and experiences of racialized students in entry-level health professional education programs: a scoping review protocol
Authors: Arulchelvam Sarah , Rana Manshi , Jexy Joshua John , Kugathasan Siyani , Sohal Sukham , Ye Jocelyn , Young Nicholas , Dhir Jasdeep 
Published: January 2025

Objective:
The objective of this scoping review will be to determine the breadth of literature exploring the perspectives and experiences of racialized students in entry-level health professional education programs.

Introduction:
Despite the implementation of equity, diversity, and inclusion (EDI) policies and alternative admission criteria for minority students, racialized minorities continue to be underrepresented and have the highest attrition rate in health professional education programs. Furthermore, the students who eventually work in health care settings report experiences of microaggression and prejudice. By not exploring the experiences of racialized students in health professional programs, EDI policies and curricula risk becoming irrelevant or performative.

Inclusion criteria:
Studies exploring the perspectives and experiences of racism and discrimination, or related concepts, among non-dominant ethno-racial (eg, Indigenous, Black, South Asian, Asian, Hispanic, Pacific Islander), entry-level health professional students will be included. Experimental study designs as well as observational and qualitative studies will also be included.

Methods:
The review will be conducted in line with the JBI methodology for scoping reviews. Five databases will be searched, namely, MEDLINE (Ovid), Embase (Ovid), CINAHL (EBSCOhost), ERIC, and Global Health (Ovid), with no limitations on language or year. A gray literature search of relevant websites will also be conducted. Two reviewers will independently screen articles against the inclusion criteria and extract and summarize data. Disagreements will be resolved through discussion or with a third reviewer.

Review registration:
Details of this review project can be found in Open Science Framework https://osf.io/4bhg6
Short-Term Changes of Anxiety in Adolescent Female Athletes
Authors: Young Julie A. , Quatman-Yates Catherine C. , Mohr Drew , Butz Catherine , Allison Katherine E. , Onate James A. , Valasek Amy E. 
Published: January 2025

Anxiety is common in adolescents but short-term changes in anxiety have not been studied. The purpose of this observational study was to describe how anxiety changes in high school female athletes over 16 weeks. Participants completed the Generalized Anxiety Disorder-7 (GAD-7) every other week. We categorized participants as elevated if their GAD-7 score was >9 and a score change of ≥4 classified those with clinically meaningful anxiety change. Of the 28 participants, 30% reported elevated GAD-7 scores at least once and 80% reported anxiety changes ≥4. Athletic trainers are poised to monitor anxiety and can promote optimal mental health by referring those with elevated GAD-7 scores for timely intervention.
Examining the Contradictions Between the Theory and Practice of Physical Literacy Assessment: Toward a New Assessment Framework
Authors: Fang Xinyuan , Zhang Zhen 
Published: January 2025

Purpose: With the development of physical literacy assessment tools such as Passport for Life and Physical Literacy Assessment for Youth, physical literacy assessment has become a hot topic in current academic research, and its assessment determines whether or not it can be put into practice, as well as leading the way in the design and implementation of pedagogical methods. Therefore, reviewing and analyzing currently available physical literacy assessment tools is valuable. Method: Based on the above, two scientific questions arise: (a) What exactly does the current physical literacy assessment tools measure, and does this contradict the original intent of physical literacy? (b) Does the current physical literacy assessment tool contradict the philosophical foundations? We reviewed physical literacy assessment tools and used a systematic review to search the Web of Science core collection. Results: (a) The results of physical literacy assessment tools are contradictory to their original purpose and cannot truly reflect the level of physical literacy. (b) Physical literacy assessment tools are contradictory to their philosophical foundations. Discussion/Conclusion: To solve these two problems, this paper draws on Wiggins’ “authentic assessment” paradigm. It proposes a new physical literacy assessment framework utilizing the Sports Game Plus Scale, which provides a reference for developing future physical literacy assessment tools.
Judgment of Learning in a Motor Task
Authors: Chatzipanteli Athanasia , Zafeiroudi Aglaia , Fotiadis Alexandros , Krommidas Charalampos 
Published: January 2025

Purpose: This study examined the judgments of learning in a motor task. Methods: Participants were 54 students, 33 fifth graders and 21 sixth graders. Students had to perform two trials in volleyball serve, one after the volleyball unit was complete and the second 10 days later. Before each trial, they had to estimate their score according to the American Alliance for Health, Physical Education, Recreation, and Dance test. Results: Statistical analysis showed that the majority of students overestimated their abilities. More specifically, fifth graders’ immediate judgments were more accurate than delayed ones, as they were more overconfident in the second trial. On the other hand, delayed judgments were more accurate in sixth graders, as they were more overconfident than fifth graders in the first trial. It should be noted, finally, that all students had better scores in their actual performances in the second trial. Conclusions: Physical educators and coaches could use judgments of learning to boost students’ ability to assess themselves more accurately and improve learning.
Psychometric Properties of the Basketball Throw Test as a Health-Related Field-Based Measure of Muscular Strength for Use With Adolescents in School Settings
Authors: Belhaidas Mohammed B. , Tahar Tahar , Yan Jin , Atallah Ahmed , O’Keeffe Brendan T. , Eather Narelle 
Published: January 2025

Purpose: This study evaluated the objectivity, test/retest reliability, and validity of the Basketball Throw Test (BTT) test administered in the school setting by physical education teachers for assessing the upper-body muscular strength in adolescents and its relationship with body mass index. Method: Four hundred and seventy Algerian adolescents (223 males and 247 females; mean age = 14.71 ± 1.95 years) performed the BTT on two occasions and the handgrip test (criterion). Convergent reliability was estimated using an intraclass correlation coefficient (ICC2,1) and graphically examined following the Bland–Altman approach. Pearson correlation coefficient employed to determine validity. Results: The BTT demonstrated excellent objectivity ICC2,1 = .963 (95% confidence interval [.948, .973]) and test–retest reliability ICC2,1 with values from .994 to .997 (95% confidence interval [.992, .997]). The Bland–Altman plot confirmed reliability results. The BTT was positively correlated with handgrip test (r = .75, p < .001). Conclusion: The BTT is an objective, valid, and reliable measure of adolescents’ upper-body muscular strength
Report of two severe cases of Japanese encephalitis virus in travelers returning to France, 2023-2024: clinical, diagnostic, and public health implications
Authors: Ayhan Nazli , Pezzi Laura , Do Rego Hermann , Thy Michael , Devatine Sandra , Melica Giovanna , Gautier Matthieu , Klitting Raphaelle , Brichler Ségolène , Durand Guillaume André , de Lamballerie Xavier , Grard Gilda 
Published: January 2025

ABSTRACT

Background

Japanese encephalitis virus (JEV) is a mosquito-borne pathogen responsible for Japanese encephalitis (JE) clinical cases in Asia and the Western Pacific. Non-JE-vaccinated patients can develop potentially life-threatening neurologic forms.

Case Summary

Here, we present two severe, non-fatal JEV cases in returning travelers from Cambodia (Case 1) and Cambodia and Vietnam (Case 2), imported to France in 2023–2024. Neither patient was vaccinated, and both presented neurologic symptoms requiring hospitalization. Cases were confirmed by RT-qPCR, IgM, and IgG rise and seroneutralization. Interestingly, this is the second report to describe the detection of the JEV genome in urine by RT-qPCR.

Conclusion

This study highlights the critical importance of JE vaccination and the implementation of other personal protective measures to avoid mosquito bites when traveling to a JEV-endemic region.
Botulinum toxin type A for the treatment of patients with post-stroke spasticity in Thailand: cost-utility and budget impact analysis
Authors: Hadnorntun Phorntida , Prawjaeng Juthamas , Kongmalai Tanawan , Tanvijit Phakamas , Chueluecha Chuenchom , Jintakul Nawarat , Saringcarinkul Tipyarat , Srinonprasert Varalak , Kumthornthip Witsanu , Leelahavarong Pattara 
Published: January 2025

ObjectivesTo evaluate the cost-utility of botulinum toxin A (BoNT-A) for treating upper limb (UL) and lower limb (LL) post-stroke spasticity.DesignUsing a Markov model, adopting a societal perspective and a lifetime horizon with a 3% annual discount rate, the cost-utility analysis was conducted to compare BoNT-A combined with standard of care (SoC) with SoC alone. Costs, utilities, transitional probabilities and treatment efficacy were derived from 5-year retrospective data from tertiary hospitals and meta-analysis. Uncertainty analyses were performed.SettingTertiary hospitals in Thailand.ParticipantsCohort of post-stroke patients aged 55 years with UL or LL spasticity and a Modified Ashworth Scale score ≥1+.InterventionsBoNT-A (abobotulinumtoxinA: aboBoNT-A, onabotulinumtoxinA: onaBoNT-A or prabotulinumtoxinA: praBoNT-A) combined with SoC versus SoC alone.Primary outcome measuresExpected life years, quality-adjusted life years (QALYs), costs and incremental cost-effectiveness ratios (ICERs), considering a cost-effectiveness threshold of 160 000 THB (US$4468) per QALY gained.ResultsThe combination of aboBoNT-A and SoC yielded the highest QALYs gained (0.013 for UL and 0.11 for LL), followed by onaBoNT-A and SoC and praBoNT-A and SoC. The additional costs for treating UL and LL cases were highest for onaBoNT-A US$75 and US$95, respectively, followed by aboBoNT-A and praBoNT-A. ICER values for treating UL with aboBoNT-A, onaBoNT-A and praBoNT-A ranged from US$4669 to US$7541 per QALY. For LL treatments, aboBoNT-A and onaBoNT-A had ICER values ranging from US$7072 to US$15 182 per QALY. Integrating BoNT-A treatment delivery into the healthcare system would require a budget outlay of approximately US$413 246–US$966 103 that may vary annually by an additional US$50 260–US$335 064.ConclusionBoNT-A effectively reduces focal spasticity and improves quality of life in post-stroke patients. However, its cost-effectiveness in Thailand necessitates price negotiations as a condition for inclusion in the pharmaceutical reimbursement list.
Implementing and evaluating the comprehensive integration of physical activity into a major health system: study design and protocol
Authors: Stoutenberg Mark , Estabrooks Paul A , Brooks John M , Jindal Meenu , Wichman Christopher , Rosemeyer James , Schumacher Leah M , McNulty Lia K , Ewing Alex , Eskuri Stephanie , Bennett Frankie , Trilk Jennifer L 
Published: January 2025

IntroductionThe healthcare sector has great potential for promoting physical activity (PA) for chronic disease prevention, treatment and management; however, multiple adoption and implementation barriers exist, ranging from practice integration to information flow. In 2016, Exercise is Medicine Greenville (EIMG), a comprehensive clinic-to-community approach that involves PA assessment, recommendation and/or prescription and provider-based referral of patients to community-based PA programmes, was launched by Prisma Health in Greenville, South Carolina, USA. Since inception, variability has emerged in adoption and implementation, impacting patient reach, referral rates and engagement in the community-based PA programmes, highlighting the need for closer evaluation and refinement of strategies to maximise programme impact.Methods and analysisThis pragmatic study will examine the adoption, implementation and reach of EIMG. 20 Prisma Health primary care clinics will be invited to adopt EIMG. In Phase I, adopting clinics will receive a standardised EIMG instructional video followed by EIMG activation, allowing providers to refer eligible patients to a 12-week evidence-informed PA programme offered at local community facilities. In Phase II, adopting clinics will receive a more in-depth EIMG onboard training. At adopting clinics, referral rates of eligible patients will be tracked over both phases (each lasting 4 months). A mixed-methods approach will explore factors related to EIMG adoption, achieving optimal implementation and reach, and patient enrolment in the PA programmes. The Reach, Effectiveness, Adoption, Implementation and Maintenance framework will inform the assessment of implementation outcomes, while the integrated Promoting Action on Research Implementation in Health Services framework will be used to explore contextual factors influencing patient-level and clinic-level outcomes.Ethics and disseminationWe received ethical approval to conduct this study from the Prisma Health IRB Committee A (#1963762). The results of this study have the potential to significantly enhance clinical practice and improve health outcomes related to integrating a clinic-to-community PA model in health systems to connect patients with community-based PA resources. Information gained from this study will lead to the refinement of a generalisable approach to inform future implementation strategies on optimising and scaling up the integration of comprehensive PA models in US health systems and be disseminated through conference presentations, publication in peer-reviewed journals and direct work with health systems.Trial registration numberNCT06073041.