bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of mesh bilaterally sacrospinous ligament suspension versus laparoscopic sacrocolpopexy in patients with uterine prolapse
Authors: Wang Gensheng , Li Qing , Xu Hongling , Zhao Zhu , Wang Dan , Zhang Yuyan , Gao Liuqin , Chen Zaoqin 
Published: May 2023

Abstract
Objective The aim in this study was to evaluate the efficacy of mesh bilaterally sacrospinous ligament suspension (MSSLS), as compared with laparoscopic sacrocolpopexy (LSC) in patients with uterine prolapse.Methods Our study evaluated 98 patients with uterine prolapse in our hospital from January 2021 to January 2023. Patients enrolled were equally divided into the study group (treated with MSSLS operation) and the control group (treated with LSC operation) by random number table. The operation conditions (including operation time, bleeding volume, indwelling catheter time, exhaust time, and hospital stay), stage of pelvic organ prolapse, postoperative recurrence rate, pain degree, quality of life, postoperative pelvic floor function, impact of sexual life, complications and recurrence rate were recorded.Results The study group demonstrated a marked reduction in the operation time, bleeding volume, indwelling catheter time, exhaust time and hospital stay when comparing with the control group (P < 0.05). Before operation, no significant difference was observed in Aa, Ba, Ap, Bp and C between the two groups (P > 0.05), while six months after operation, the five indexes in the study group were significantly lower than those in the control group (P < 0.05). The pain degree showed no significant difference between the two groups before operation (P > 0.05), while was evidently lower in the study group than control group 6 months after operation (P < 0.05). In terms of quality of life, pelvic floor function, and quality of sexual life, no significant difference was found before, 6 months and 12 months after operation (P > 0.05). All patients were followed up for 12–14 months, with an average follow-up time of (13.02 ± 1.36) months. The incidence of complications in the study group was significantly lower than the control group (P < 0.05). However, there was no recurrence in both groups, hence the difference was not statistically significant (P > 0.05).Conclusion MSSLS is a safe and effective treatment for uterine prolapse that significantly avoided complications and recurrence, which is believed available for wide application in clinical practice.
Cost-effectiveness analysis of Tislelizumab vs. Camrelizumab for the treatment of second-line locally advanced or metastatic esophageal squamous cell carcinoma
Authors: Chen Pingyu , Fu Chang , Shen Lin , Fei Zhengyang , Luo Mengjie , Chen Yanqiu , Li Hongchao 
Published: May 2023

AbstractBackgroundTislelizumab is a selective PD-1 inhibitor with efficacy proven in locally advanced or metastatic esophageal squamous cell carcinoma. The study aims to evaluate the cost-effectiveness of tislelizumab versus camrelizumab as the second-line treatment in locally advanced or metastatic esophageal squamous cell carcinoma (ESCC) patients in China.MethodsFrom the perspective of China’s healthcare system, the partitioned survival model with three health states was established in a 3-week cycle and a lifetime horizon. Anchored matching adjusted indirect comparison was used for survival analyses based on individual patient data from RATIONALE 302 trial and the published ESCORT study due to the lack of head-to-head clinical trials. Only direct medical costs were included. Costs and utility values were derived from local charges, the published literature, and related databases. Sensitivity analyses and a scenario analysis were also performed to verify the robustness of the model results.ResultsCompared with camrelizumab monotherapy, tislelizumab monotherapy incurred a lower lifetime cost ($8,346 vs. 8,851) and yielded higher quality-adjusted life-years (QALYs) (0.87 vs. 0.63), which resulted in an incremental cost-effectiveness ratio (ICER) of -$2,051/QALY. Tislelizumab monotherapy is a dominant option over camrelizumab monotherapy in China. The three primary parameters upon which this result was most sensitive were the unit cost of camrelizumab, the unit cost of tislelizumab, and the duration of reactive cutaneous capillary endothelial proliferation (RCCEP). According to the probabilistic sensitivity analysis (PSA), tislelizumab monotherapy was 100% cost-effective when the WTP was 1–3 times GDP per capita in China($11,207/QALY∼$33,621/QALY). Scenario analysis showed that the result was consistent.ConclusionTislelizumab monotherapy is a dominant option compared with camrelizumab monotherapy as the second-line treatment for locally advanced or metastatic ESCC in China.
Beyond Traditional Methods: Unleashing the Power of Neural Networks in Predicting Early Recurrence and Mortality after Curative Surgery in Colorectal Cancer Patients
Authors: Alinia Shayeste , Mahmoudi Leila , norouzi Solmaz , Roshanaei Ghodratollah , Safari Maliheh , Asghari-Jafarabadi Mohammad 
Published: May 2023

Abstract
Background: Medical research frequently relies on Cox regression to analyze the survival distribution of cancer patients. Nonetheless, in specific scenarios, neural networks hold the potential to serve as a robust alternative. In this study, we aim to scrutinize the effectiveness of Cox regression and neural network models in assessing the survival outcomes of patients who have undergone treatment for colorectal cancer.
Materials and Methods: We conducted a retrospective study on 284 colorectal cancer patients who underwent surgery at Imam Khomeini clinic in Hamadan between 2001 and 2017. The data was used to train both Cox regression and neural network models, and their predictive accuracy was compared using diagnostic measures such as sensitivity, specificity, positive predictive value, accuracy, negative predictive value, and area under the receiver operating characteristic curve (ROC). The analyses were performed using STATA 17 and R4.0.4 software.
Results: The study revealed that the best neural network model had a sensitivity of 74.5% (95% CI: 61.0-85.0), specificity of 83.3% (65.3-94.4), positive predictive value of 89.1% (76.4-96.4), negative predictive value of 64.1% (47.2-78.8), AUC of 0.79 (0.70-0.88), and accuracy of 0.776 for death prediction. For recurrence, the best neural network model had a sensitivity of 88.1% (74.4%-96.0%), specificity of 83.7% (69.3%-93.2%), positive predictive value of 84.1% (69.9%-93.4%), negative predictive value of 87.8% (73.8%-95.9%), AUC of 0.86 (0.78-0.93), and accuracy of 0.859. The Cox model had comparable results, with a sensitivity of 73.6% (64.8-81.2) and 85.5% (78.3-91.0), specificity of 89.6% (83.8-93.8) and 98.0% (94.4-99.6), positive predictive value of 84.0% (75.6-90.4) and 97.4% (92.6-99.5), negative predictive value of 82.0% (75.6-90.4) and 88.8% (0.83-93.1), AUC of 0.82 (0.77-0.86) and 0.92 (0.89-0.95), and accuracy of 0.88 and 0.92 for death and recurrence prediction, respectively.
Conclusion: In conclusion, the study found that both Cox regression and neural network models are effective in predicting early recurrence and death in patients with colorectal cancer after curative surgery. The neural network model showed slightly better sensitivity and negative predictive value for death, while the Cox model had better specificity and positive predictive value for recurrence. Overall, both models demonstrated high accuracy and AUC, indicating their usefulness in predicting these outcomes.
Evaluation of expression profiles due to garlic intake in young men and women
Authors: Brusgaard klaus , Burton Mark 
Published: May 2023

Abstract
Garlic has shown many and diverse beneficial effects on health. Focus has been on which compounds have effect. We analyze the transcriptional consequences of digesting limited amounts of raw garlic and analyze the overall outcome. This study focuses on the reported health related effects of garlic digestion in relation to the observed transcriptional changes to identify and understand the eventual casual outcome. We utilize a non-hypothesis driven global approach to analyze the transcriptional consequences of eating moderate amounts of garlic in a group of young people. Analyzing changes in gene expression of RNA extracted from peripheral blood using microarrays and performing unsupervised principal components analysis to generate heatmaps. Pathway analysis was performed using fast gene set enrichment analysis with KEGG, Reactome and Gene Ontology defined gene sets as input. A number of significant pathway changes was observed. When correcting for gender difference a possible relation to beneficial effects observed in both men and women was observed. Especially the gender specific immune dimorphism upon garlic digestion Our results support the notion of a link between olfaction, neurology and the immune system and implies that a common mechanism explain the link between garlic ingestion and the previous reported pleiotropic positive effects.
Obesity aggravates ischemia–reperfusion injury-induced acute kidney injury in mice
Authors: Silva Igor O. , Menezes Nicole K. , Jacobina Heloisa D. , Parra Antonio C. , Souza Felipe L. , Castro Leticia Cardoso , Roelofs Joris , Tammaro Alessandra , Gomes Samirah Abreu , Sanches Talita R. , Andrade Lucia 
Published: May 2023

Abstract

In critically ill patients, overweight and obesity are associated with acute respiratory distress syndrome and acute kidney injury (AKI). Although obesity is known to increase oxidative stress, worsen inflammation, and induce a state of Klotho deficiency, its effect on ischemia–reperfusion injury (IRI)-induced AKI is unknown. We hypothesized that obesity would aggravate renal IRI in mice. We fed mice a standard or high-fat diet for eight weeks. The mice were divided into four groups and submitted to sham surgery or IRI: obese, normal, normal + IRI, obese, and obese + IRI. All studies were performed 48 h after the procedures. Body weights were higher in the obese and obese + IRI groups than in the normal and normal + IRI groups, respectively. Serum glucose and cholesterol did not differ among the groups. Creatinine clearance also did not differ among the groups. Survival and urinary osmolality were lower in the obese + IRI group than in the normal + IRI group, whereas urinary neutrophil gelatinase-associated lipocalin levels, tubular injury scores, and caspase 3 expression were higher. In the obese + IRI group, the PCNA-positive cell counts were highest, as were the levels of oxidative stress (urinary levels of thiobarbituric acid-reactive substances and renal heme oxygenase-1 protein expression), and renal Klotho protein expression was lowest. Notably, macrophage infiltration was greater in the obese group mice than in the normal group mice, despite the fact that none of those mice were induced to AKI. In conclusion, greater oxidative stress might aggravate IRI in obese individuals, and Klotho could be a therapeutic target in those with AKI.
Understanding inequality in health and other expenditures in Kerala
Authors: Kattuman Paul , Nair Ashwin 
Published: May 2023

Abstract
There is renewed interest in understanding economic inequality motivated by unprecedented extremes in the top shares of expenditure, income and wealth, worldwide. We present an empirical account of changes in household expenditure distributions and inequality in Kerala with particular emphasis on health expenditure. The analysis of data for a panel of households surveyed in 2004-5 and 2011-12 indicates that, overall, inequality in household expenditure and income in Kerala declined between those years, contrary to the widely held view. At the same time, inequality among households at the top end of the expenditure distribution is exceptionally extreme for health expenditures, to the point that an infinite population variance cannot be rejected. Conditioned on total household expenditure, health expenditure is highly unequal relative to other types of expenditures. Evidence suggests that households’ higher health expenses go hand in hand with curtailing other types of expenditures, and that the trade-off is more pronounced among households at the lower end of total household expenditure. Remarkably extreme upper-tail expenditure inequality that characterises quasi-public goods in Kerala carries implications for social welfare and health policy.
Severe generalized periodontitis as a risk factor for serious outcomes of COVID- 19: a structural equation modelling approach
Authors: Costa Camila Alves , Vilela Ana Carolina Serafim , Oliveira Suzane Aparecida , Perazzo Matheus França , Leles Cláudio Rodrigues , Costa Nádia Lago 
Published: May 2023

Abstract
Objectives This study aimed to assess the periodontal condition in COVID-19 patients and determine the multidirectional association between severe generalized periodontitis and COVID-19 outcomes.Materials and Methods Periodontal conditions were clinically assessed in 214 hospitalized patients due to COVID-19, and the course of the disease was registered prospectively from hospital admission. A conceptual model was developed to predict the impact of severe generalized periodontitis on poor symptomatology, intensive care unit (ICU) admission, and death of COVID-19 patients. The model was tested using Structure Equation Modeling (SEM) to predict severe COVID-19 outcomes as affected by periodontitis, when including comorbidities, patient's age, and smoking habit as potential confounding variables.Results Severe generalized periodontitis was diagnosed in 51 (23.8%) patients, and 52 (26.8%) died due to aggravated COVID-19. SEM analysis revealed that severe generalized periodontitis was positively associated with severe-critical COVID-19 symptoms in hospitalized patients [standard coefficient (sc) = 0.166; 95%CI = 0.014–0.319; p = 0.032] and influenced by confounders (age and smoking). Significant associations were observed in the pathway from presentation of severe-critical symptoms to ICU admission (sc = 0.995; 95%CI = 0.924–1.065; p < 0.001) and death (sc = 0.512; 95%CI = 0.392–0.631; p < 0.001].Conclusions Findings suggest that severe generalized periodontitis may play a role in the aggravation of COVID-19 symptoms and the risks of subsequent need for ICU admission and death.Clinical Relevance: These results elucidate the probable course of the relationship between periodontitis and COVID-19, emphasizing the importance of periodontitis as a risk factor and accentuating the needs of prevention and handle of periodontal disease in the inhibition of COVID-19 complications.
Characterisation of thermally stable bamboo fibre sheet loaded with cobalt ferrite nanoparticles
Authors: - Sheetal , Kaur Pawandeep , Kaur Varinder 
Published: May 2023

Abstract
Magnetic fibres were synthesized by lumen loading method using bamboo fibres as a cellulose source, as well as cobalt ferrites nanoparticles with polyethyleneimine (PEI) as a retention aid. Bamboo fibre is a significant fibrous raw material, and cobalt ferrites particles are suitable for magnetic modification. The resulting composite retained the intrinsic features of cellulose fibre while gaining the magnetic properties of nanoparticles. Cobalt ferrites nanoparticles were synthesised using a sol-gel technique and coated with tetraethyl orthosilicate (TEOS) molecules before being functionalized with polyethyleneimine (PEI). Scanning electron microscopy (SEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD), Fourier transform infrared (FTIR), and a vibrating sample magnetometer (VSM) were used to characterise the samples The nanoparticles produced had an inverse cubic spinel crystalline structure and exhibited ferrimagnetic activity. The coercivity value for Modified fibre produced is greater than 200 Oe, indicating that it may be utilised for magnetic recording.
Comprehensive Analysis of Nematode-Plant-Mycorrhizal Fungus System for Bio Based Products Development
Authors: Garita Sebastián Andrés , Bernardo Valeria Fernanda , Gonzalez Matias , Arango María Cecilia , Ruscitti Marcela 
Published: May 2023

Abstract
Nacobbus aberrans is a plant parasite that causes significant economic losses in the Americas and is considered a quarantine pest in many countries worldwide. Some mycorrhizal fungi have been shown to have the ability to decrease the population of this pathogen, however, most studies have focused on quantifying N. aberrans population variations without considering the effects on the crop or the possibilities of survival of the mycorrhizal fungi. This work consisted of selecting a mycorrhizal fungus strain based on a comprehensive analysis of the Nematode-Plant-Mycorrhizal Fungus system. The test was conducted on Solanum licopersicum, grown in 10-liter pots. The mycorrhizal fungi inoculation was done at planting, and the nematode inoculation was done at the time of transplanting. The evaluated strains were Funneliformis moseeae, Rhizoglomus intraradices A2, and Rhizoglomus intraradices B1. During the crop cycle, net photosynthesis and stomatal conductance were quantified. After removing the plants, biomass weight, stem diameter, chlorophyll concentration, soluble proteins, malonyldialdehyde, proline, phenol content, and relative conductivity were determined in leaf and root tissues. Regarding the nematode population, the number of eggs, mobile forms, final population, and reproduction factor were analyzed. Regarding the mycorrhizal fungus, the percentage of mycorrhization and viability of fungal structures for each treatment were quantified. The analysis of all the mentioned variables contributed to determine that the strain with the highest capacity to compensate for the negative effects of parasitism in the crop, with high possibilities of survival in the substrate and capable of decreasing the population of Nacobbus aberrans, is Funneliformis moseeae.
OSGIN1 is a novel TUBB3 regulator that promotes tumor progression and gefitinib resistance in non-small cell lung cancer
Authors: Xie Xiaomeng , Laster Kyle Vaughn , Li Jian , Nie Wenna , Yi Yong Weon , Liu Kangdong , Seong Yeon-Sun , Dong Zigang , Kim Dong Joon 
Published: May 2023

Abstract
Background
Oxidative stress induced growth inhibitor 1 (OSGIN1) regulates cell death. The role and underlying molecular mechanism of OSGIN1 in non-small cell lung cancer (NSCLC) are uncharacterized.
Methods
OSGIN1 expression in NSCLC samples was detected using immunohistochemistry and Western blotting. Growth of NSCLC cells and gefitinib-resistant cells expressing OSGIN1 or TUBB3 knockdown was determined by MTT, soft agar, and foci formation assays. The effect of OSGIN1 knockdown on in vivotumor growth was assessed using NSCLC patient-derived xenograft models and gefitinib-resistant patient-derived xenograft models. Potentially interacting protein partners of OSGIN1 were identified using IP-MS/MS, immunoprecipitation, and Western blotting assays. Microtubule dynamics were explored by tubulin polymerization assay and immunofluorescence. Differential expression of signaling molecules in OSGIN1 knockdown cells was investigated using phospho-proteomics, KEGG analysis, and Western blotting.
Results
We found that OSGIN1 is highly expressed in NSCLC tissues and is positively correlated with low survival rates and tumor size in lung cancer patients. OSGIN1 knockdown inhibited NSCLC cell growth and patient-derived NSCLC tumor growth in vivo. Knockdown of OSGIN1 strongly increased tubulin polymerization and re-established gefitinib sensitivity in vitro and in vivo. Additionally, knockdown of TUBB3 strongly inhibited NSCLC cell proliferation. Mechanistically, we found that OSGIN1 enhances DYRK1A-mediated TUBB3 phosphorylation, which is critical for inducing tubulin depolymerization. The results of phospho-proteomics and ontology analysis indicated that knockdown of OSGIN1 led to reduced propagation of the MKK3/6-p38 signaling axis.
Conclusions
We propose that OSGIN1 modulates microtubule dynamics by enhancing DYRK1A-mediated phosphorylation of TUBB3 at serine 172. Moreover, elevated OSGIN1 expression promotes NSCLC tumor growth and gefitinib resistance through the MKK3/6-p38 signaling pathway. Our findings unveil a new mechanism of OSGIN1 and provide a promising therapeutic target for NSCLC treatment in the clinic.
A quantitative method for determination of colistin E2 methane sulphonate sodium (CMS E2) in human plasma by 15N-labeled colistin E2
Authors: Feng Jun , Xu Hongjiang , Zhang Jinhua , Lu Jianguang , Wenjie Zhao , Yanan Li 
Published: May 2023

Abstract
The single-component colistin E2, with superior antibacterial activity and lower toxicity, was being developed as the latest generation of polymyxin drugs. However, colistin E2 has not been tested quantitatively in biological matrices. In this study, based on the quantitative detection of CMS and colistin by Zhao et al., 15N-labeled colistin E2 was used as an internal standard (IS) for a more accurate quantitative detection of CMS E2 in human plasma. A rapid ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) assay method was developed for determination of CMS E2 and colistin E2 in human plasma. After pretreatment of plasma samples by 96-well SPE Supra-Clean Weak Cation Exchange (WCX) plate, the formed colistin E2 was detected and quantified by UHPLC–MS/MS system. All plasma lots were found to be free of interferences with the analyte. The matrix has no effect on the quantitation of the analyte. No significant effect of the carryover was observed. The dilution integrity was demonstrated in plasma samples without the loss of accuracy and precision. The lower limit of quantification (LLOQ) was 0.0300 mg/L for colistin E2 in plasma with accuracy (relative error, 5.1 ~ 12.7%) and precision (relative standard deviation, -5.7 ~ 9.3%). Stability of CMS E2 and colistin E2 was demonstrated in biological samples before and during sample treatment, and in the extract. Furthermore, this method was successfully applied to the analysis of plasma samples obtained from Chinese healthy volunteers receiving a CMS E2 dose of 5 mg/kg. In conclusion, the detection method was characterized by speed and high accuracy, which laid a solid foundation for the subsequent development of CMS E2 drug.