bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trait–environment relationships of hydroids (Cnidaria: Hydrozoa) across large spatial and environmental gradients in the Atlantic Ocean
Authors: Fernandez Marina Oliveira , Jaimes‐Becerra Adrian , Marques Antonio Carlos 
Published: January 2023

AbstractAimThe aim was to test trait–environment relationships in hydroids across large spatial and environmental gradients and to evaluate associations between traits, environmental variables, space and phylogeny.LocationAtlantic Ocean and adjacent polar seas.Time periodPresent day.Major taxa studiedHydrozoa.MethodsTrait–environment relationships and their spatial and phylogenetic contexts were assessed in hydroids by using a combination of a fourth‐corner test and extended‐RLQ approach, in a multivariate ordination method that uses five matrices: (1) species across sites, (2) environmental data across sites, (3) traits across species, (4) latitude and longitude for each site, and (5) phylogenetic distance among species. We based our analyses on 3680 records of 431 species distributed at 1440 sites, 16 species traits, nine environmental variables and a phylogenetic tree of the studied species.ResultsHydroid traits are significantly correlated with environmental variables and geographical space, and hydroid phylogeny is correlated with the environment and geographical space. More evidently, we observed an increased presence of larger species (taller, more branched, with greater base diameter and polysiphonic) at sites richer in nitrate and silicate, with higher dissolved oxygen, lower temperatures, lower salinities and lower current velocities. The observation of phylogenetically related species with similar traits and distributions corroborates that historical processes interact with the environment in determining community assembly and explaining patterns of distribution of species traits.Main conclusionsSeveral environmental variables combined affect the distribution of hydroid traits, which are also influenced by spatial and historical factors. In the first analysis of its kind for hydrozoans, we have provided an overview of how hydroids are distributed across environments according to their traits and revealed the spatial and phylogenetic components of these trait–environment relationships.
Apoptosis versus necrosis in tubal ectopic pregnancies following Methotrexate
Authors: Gil Yaron , Zubkov Asia , Balayla Jacques , Cohen Aviad , Levin Ishai 
Published: January 2023

AbstractMethotrexate administration for the treatment of tubal ectopic pregnancies has been shown to cause tubal mass enlargement. Our hypothesis was that, by administrating Methotrexate, a local necrotic reaction occurs, leading to hematoma formation and eventually fallopian tube rupture. Salpingectomy specimens were collected, analysed and divided into three equal groups: patients who received Methotrexate but who ultimately failed medical treatment, patients who had a viable ectopic pregnancy and patients with a self‐resolving ectopic pregnancy that were operated due to other medical indications. The specimens were dyed using the Cleaved Caspase‐3 (Asp175) Rabbit mA. Specimens were divided into three equal groups and analysed. The patients in self‐resolving ectopic pregnancy group were older and had more pregnancies. Rates of apoptosis were found to be less than 1% per slide. Necrosis was not evident in any of the pathological specimens. It seems Methotrexate administration does not lead to a significant tubal necrotic reaction. Further studies are required.
Family and work‐related risk factors in children's social–emotional well‐being and parent–educator cooperation in flexibly scheduled early childhood education and care
Authors: Koivula Merja , Räikkönen Eija , Turja Leena , Poikonen Pirjo‐Liisa , Laakso Marja‐Leena 
Published: January 2023

AbstractNon‐standard work schedules (NSWS) have become typical, but their associations with childcare arrangements and children's well‐being are unknown. This study explores how risk factors are associated with the social–emotional well‐being of girls and boys using flexibly scheduled early childhood education and care. Furthermore, the study investigates whether well‐functioning cooperation between parents and educators buffers the negative effects of the risk factors. This study, which is a part of a larger survey carried out in three European countries, reports Finnish parents' (N = 146) perspectives. The results showed that high parental stress was associated with low child well‐being. Strong parent–educator cooperation positively impacted both boys' and girls' social–emotional well‐being. The risk factors of reconciling work and family life had negative associations with children's well‐being and the fulfilment of their basic needs. The results illustrate the complex interrelations between children's well‐being, risk factors relating to NSWS and the buffering effect of protective factors.
Pleistocene megafaunal extinction in the grasslands of Junín‐Peru
Authors: Rozas‐Davila Angela , Rodbell Donald T. , Bush Mark B. 
Published: January 2023

AbstractAimTo determine the timing of megafaunal extinction in the high plains of Peru and also to determine if the timing was delayed in grasslands compared with previously published forested settings to dissociate the effects of succession from human or climatic impacts.LocationThe Junín Plateau, Peru.TaxonFlowering plants and Ascomycetes.MethodsThe sediments used in this analysis were collected from the edge of Lake Junín, Peru. Eleven ages derived from 14C accelerator mass spectrometry provide a chronology. We provide a paleoecological reconstruction of past climate and vegetation change, fire history and megafaunal herbivore presence from c. 20,000–7000 years ago based on fossil pollen, charcoal and Sporormiella spores. Data were analysed using multivariate analysis and Bayesian change point analysis.ResultsMegafaunal populations appear to have been positively impacted by dry climatic oscillations until c. 15,200 years ago. A reduction in Sporormiella abundance between 13,000 and 12,300 years before the present coincides with increased charcoal abundance and is identified as the period of megafaunal population collapse leading to extinction. The timing of the extinction does not differ substantially from that observed in wooded Andean settings.Main ConclusionThe timing of the collapse of megafaunal populations in high grasslands was very similar to that of lower, now‐forested settings. Upticks in fire activity, during what is generally seen to be a wet period, formed the backdrop to extinction and are strongly associated with human activity.
Comparing dominance relationships and movement of native marble trout (Salmo marmoratus) and introduced rainbow trout (Oncorhynchus mykiss)
Authors: Pengal Polona , Cokan Blaž , Økland Finn , Höjesjö Johan , Tambets Meelis , Thorstad Eva Bonsak 
Published: January 2023

AbstractBehaviour observations of the endangered native marble trout (Salmo marmoratus, Cuvier, 1829) and introduced rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) in the laboratory and in a tributary to the Idrijca River in Slovenia were combined to study the movements and dominance relationships between individuals of the two species in an open field test. Under laboratory conditions, no difference between the species was detected for neither time spent actively or distance moved. In species paired tests, rainbow trout initiated more aggressive behaviours towards marble trout than vice versa, and rainbow trout were clearly the dominant individuals. After simultaneous release in the river, marble trout immediately left the release area and spent twice as long time as rainbow trout until they settled in an area of the river; therefore, the release site was immediately occupied exclusively by rainbow trout. Thus, the dominant and aggressive behaviour of rainbow trout seen in the laboratory before release might have influenced marble trout's subsequent behaviour in the river, by marble trout leaving the areas occupied by rainbow trout and moving to locations further away from the release site. In the field, the marble trout occupied sites individually, whereas rainbow aggregated at a few locations. Rainbow trout showed higher movement activity in the morning compared to marble trout. There was a positive correlation between swimming speed in the laboratory and movement in the field for marble trout but not for rainbow trout. In conclusion, the results in this study support the need to end stocking of rainbow trout in rivers with native marble trout. To better understand the interaction between the species, and to develop efficient management plans to protect the native marble trout, reference behaviours should first be understood, and future research in sites where the two species do not co‐exist is needed. This is especially important for marble trout for which behavioural research and data are lacking.
Metataxonomic and metabolic evaluation of three water kefir microbiomes cultured in sugar cane juice
Authors: Wilches‐López Lisett , Correa‐Espinal Alexander , Pérez‐Monterroza Ezequiel José , Rojas Luisa F. 
Published: January 2023

AbstractThree water kefir grains (WKG) C, B, M with higher heterogeneity in the consortia C and M, and different biodiversity, dominated mainly by the genus Lactobacillus were selected from three Colombian locations and evaluated metataxonomically and metabolically using sugar cane juice (SCJ) as a culture medium, the molecular characterization was performed by sequencing in Illumina Miseq, and microbial diversity used Mothur platforms. The objective was to evaluate the SCJ on the consortium and the bacterial and fungal diversity immersed in the WKG. Biomass and secondary metabolites were monitored during 152 h. Growth kinetic data were fitted to the Logistic, Richards, and Gompertz models. WKG C rRNA 16S sequencing by Simpson's index (0.298) evidenced low species biodiversity, dominated by Lactobacillus sp. In C (0.986), the ITS1 marker indicated a more heterogeneous community than B and M, with Saccharomyces sp. as the predominating species. Parameters of the Gompertz model fitted to C biomass were: μmax (0.032 h−1), latency time (1.736 h), and adaptation (6.48 h). WKG consortia B showed higher acetic acid and ethanol production with 5.269 and 0.333 g/L, respectively. SCJ is a suitable medium for Lactobacillus sp. and Saccharomyces sp. strains in WKG.
Effect of rpoS on the survival and gene expression of Salmonella Enteritidis in low water activity foods
Authors: Ma Zhuolin , Li Shaoting , Xu Weiying , Chen Yingqi , Lu Ziying , Fu Rong , He Xinyi , Zhang Hongmei 
Published: January 2023

AbstractThis study evaluated the effect of the transcriptional regulator RpoS on bacterial survival, tolerance to simulated gastrointestinal conditions, and the expression of stress resistance genes in wild‐type (WT) and rpoS‐deletion (ΔrpoS) Salmonella Enteritidis CICC 21482 in low water activity (Aw) foods at 24 hr of drying and 7 days of storage. The ΔrpoS strain was produced by allelic exchange using a suicide plasmid. Bacteria were inoculated into ginger powder, nonfat milk powder, and chocolate powder in the presence of simulated gastric and intestinal fluids, and survival was measured. Gene expression was measured using real‐time quantitative polymerase chain reaction. The ΔrpoS strain was less resistant to desiccation stress than the WT strain. The bacterial tolerance to gastrointestinal conditions varied depending on food composition, and high‐fat and protein content enhanced the survival of S. Enteritidis in the gastrointestinal conditions. In addition, under low Aw conditions, rpoS was weakly correlated with the expression of fadA but strongly correlated with the expression of grpE, otsA, otsB, dnaK, proV, rpoH, and sigDE in food substrates. This study elucidates the role of rpoS in desiccation stress and serves as the basis for preventing and controlling Salmonella contamination in low Aw foods.
The parental umwelt: Effects of parenthood on sensory processing in rodents
Authors: Wilson Kerianne M. , Arquilla April M. , Saltzman Wendy 
Published: January 2023

AbstractAn animal's umwelt, comprising its perception of the sensory environment, which is inherently subjective, can change across the lifespan in accordance with major life events. In mammals, the onset of motherhood, in particular, is associated with a neural and sensory plasticity that alters a mother's detection and use of sensory information such as infant‐related sensory stimuli. Although the literature surrounding mammalian mothers is well established, very few studies have addressed the effects of parenthood on sensory plasticity in mammalian fathers. In this review, we summarize the major findings on the effects of parenthood on behavioural and neural responses to sensory stimuli from pups in rodent mothers, with a focus on the olfactory, auditory, and somatosensory systems, as well as multisensory integration. We also review the available literature on sensory plasticity in rodent fathers. Finally, we discuss the importance of sensory plasticity for effective parental care, hormonal modulation of plasticity, and an exploration of temporal, ecological, and life‐history considerations of sensory plasticity associated with parenthood. The changes in processing and/or perception of sensory stimuli associated with the onset of parental care may have both transient and long‐lasting effects on parental behaviour and cognition in both mothers and fathers; as such, several promising areas of study, such as on the molecular/genetic, neurochemical, and experiential underpinnings of parenthood‐related sensory plasticity, as well as determinants of interspecific variation, remain potential avenues for further exploration.
Fetal left ventricular myocardial performance index measured at 11–14 weeks of gestation in fetuses with an increased nuchal translucency
Authors: Sezer Salim , Oğlak Süleyman Cemil , Kaya Başak , Behram Mustafa , Gedik Özköse Zeynep , Süzen Çaypınar Sema , Acar Züat , Gezdirici Alper , Bornaun Helen 
Published: January 2023

AbstractObjectiveThis study aimed to evaluate the effect of an increase in nuchal translucency (NT) thickness on the myocardial performance index (MPI) in fetuses without cardiac anomaly in the first trimester and to determine whether a difference in MPI between those with and without trisomy 21 in these fetuses could be determined.MethodsThe study group consisted of 53 pregnancies complicated with increased NT thickness without any associated structural anomalies. Forty‐six gestational age‐matched pregnant women whose fetuses had normal NT thickness were enrolled as the control group.ResultsIn the increased NT thickness group, the mean isovolumetric relaxation time (IRT) value (0.050 ± 0.011 s) was significantly higher and the mean ejection time (ET) value (0.149 ± 0.010 s) was significantly lower than those values in the normal NT thickness group (0.045 ± 0.005 and 0.155 ± 0.009 s, p = 0.023 and p = 0.009, respectively). We found a significantly higher mean left MPI value in the increased NT thickness group (0.574 ± 0.153) versus the normal NT thickness group (0.487 ± 0.107, p < 0.001). Within the increased NT thickness group, the mean left MPI value was similar in the fetuses with normal karyotype and those with trisomy 21 (p = 0.419).ConclusionWe demonstrated a significantly greater mean MPI value in the increased NT thickness group than in the normal NT thickness group. Within the increased NT thickness group, no differences in the left MPI value in the fetuses with normal karyotype and the fetuses with trisomy 21 were found.
Associations between snoring and dental sleep conditions: A systematic review
Authors: Huang Zhengfei , Zhou Ning , Chattrattrai Thiprawee , van Selms Maurits K. A. , de Vries Ralph , Hilgevoord Antonius A. J. , de Vries Nico , Aarab Ghizlane , Lobbezoo Frank 
Published: January 2023

AbstractBackgroundIt is important for dentists to know if the presence of snoring is associated with the presence of other dental sleep conditions (e.g. obstructive sleep apnea [OSA], sleep bruxism [SB], gastroesophageal reflux disease [GERD], xerostomia and oro‐facial pain). If so, dentists could play a significant role in the early recognition and management of these conditions.ObjectivesThis systematic review aimed to: (i) investigate the associations between the presence of snoring and the presence of other dental sleep conditions; and (ii) determine if it is clinically relevant that dentists assess snoring in their population.MethodsThe literature search was performed in PubMed and Embase.com in collaboration with a medical librarian. Studies were eligible if they employed regression models to assess whether snoring was associated with other dental sleep conditions, and/or investigated the incidence of snoring in patients with other dental sleep conditions and vice versa.ResultsOf the 5299 retrieved references, 36 eligible studies were included. The available evidence indicates that the presence of snoring is associated with higher probabilities of OSA, GERD and headache. Due to limited evidence and conflicting findings, the currently available articles are not indicative of associations between the presence of snoring and the presence of SB and oral dryness.ConclusionWithin the limitations of this study, it can be concluded that the presence of snoring is associated with higher probabilities of OSA, GERD and headache. Therefore, it is clinically relevant that dentists assess snoring in their patient population.
Possible adverse effects of low‐level laser on oral and oropharyngeal cancer cells: A scope review
Authors: Xavier Maria Gabriella Apolinário , Moura Maria de Lourdes Neves de , Ribeiro Lucas Nascimento , Carvalho Marianne de Vasconcelos , Ferreira Stefânia Jeronimo 
Published: January 2023

AbstractBackgroundThe effects of laser therapy on normal cells are well known and accepted, but the impact of this therapy on malignant cells are not yet fully understood. This review aims to map and outline what the scientific literature addresses on the effects of laser therapy on malignant cells.MethodsThis review article followed the guidelines of the PRISMA‐ScR protocol, being all the search, analysis, and selection of articles based on it.ResultsAfter all application of the predetermined criteria, five studies were included, dated between the years 2013 and 2021. With the complete reading of the selected studies, 100% of the articles were classified as category III of the Agency for Healthcare as Research and Quality classification. Similar themes among the papers included were investigated and compared. In these five studies, the visible red and near infrared wavelengths were used, and energy densities varied between 1 and 5 J/cm2. It was observed that low‐level laser could alter the expression of cell proliferation and migration proteins, such as cyclin D1, E‐cadherin, and β‐catenin. In addition, changes related to increased cell viability and metabolism were also identified.ConclusionThe low‐level laser seems to positively regulate the proliferative, migratory, and viability capacity of neoplastic cells, depending on the protocol used. All these studies included in the review are equivalent to in vitro studies; the cells are not in such a complex environment as is an organized tissue, making it necessary to carry out more complex tests, such as in vivo research.
Comparative evaluation of gingival fibroblast growth on 3D‐printed and milled zirconia: An in vitro study
Authors: Zandinejad Amirali , Khurana Saumya , Liang Yongxi , Liu Xiaohua 
Published: January 2023

AbstractPurposeThe purpose of this study was to analyze the fibroblast growth and proliferation on 3D‐printed zirconia in presence and absence of porosities.Material and methodsA total of 40 bars (8 × 4 × 3) were included in this study. Thirty 3D‐printed and 10 milled zirconia samples were prepared. The 3D‐printed samples had different porosities, 0% (PZ0), 20% (PZ20), and 40% (PZ40) with 10 specimens in each group. Milled zirconia samples were used as the control (MZ). Rat gingival fibroblasts were cultured for 48 h, and the proliferation of fibroblasts on each sample in each group (n = 10) was determined by MTT assays. The differences among the four groups were compared by one‐way ANOVA. To test the significance of the observed differences between two groups, an unpaired Student's t‐test was applied. The significance level was set at p < 0.05. Qualitative analysis for the cell culture was performed using scanning electron microscopy.ResultsOne‐way ANOVA showed that the numbers of the fibroblasts among the four groups had a statistical difference. Post hoc Bonferroni test revealed that there was no significant difference between PZ0 and MZ; however, all other groups and among groups were significantly different.ConclusionsFibroblasts had a better affinity toward the MZ and PZ0 in a short period of cell culture time.
Intussusception as a rare manifestation of immunoglobulin A vasculitis: Risk factors and treatment
Authors: Acar Banu Ç , Tekin Zahide E , Güngörer Vildan , Çelikel Elif , Kurt Tuba , Tekgöz Nilüfer , Sezer Müge , Karagöl Cüneyt , Coşkun Serkan , Kaplan Melike M , Polat Merve C , Öner Nimet , Kuruç Aylin I , Erten Elif E , Şenel Emrah 
Published: January 2023

AimTo identify the risk factors associated with intussusception in children with immunoglobulin A vasculitis (IgAV)‐gastrointestinal (GI) tract involvement and to evaluate the outcomes of medical treatment and surgical intervention and the course of patients with intussusception.MethodsThis retrospective study was conducted in 157 patients under 18 years of age who were followed up with the diagnosis of IgAV‐GI tract involvement between January 2015 and September 2022. The characteristics of the patients who developed intussusception were evaluated in detail.ResultsOne hundred and fifty‐seven patients with GI tract involvement were included in the study. The mean age of patients with IgAV‐GI tract involvement was 8.7 ± 3.7 years. The female‐to‐male ratio was 1:1.5. Intussusception was detected in 14 patients (8.9%). Two patients (14.3%) underwent surgery, and the remaining 12 patients (85.7%) had their medical therapy intensified. Patients with GI tract involvement were divided into two groups as with (n = 14) and without (n = 143) intussusception. There was a statistically significant difference between the groups in the time from the onset of the first symptom of IgAV to the onset of steroids (P = 0.001). There were no statistically significant differences between the groups in age at onset of IgAV, gender distribution, erythrocyte sedimentation rate and C‐reactive protein levels.ConclusionsThe time from the onset of the first symptom of IgAV to the start of steroids is a risk factor for the development of intussusception in patients with IgAV‐GI tract involvement. In these patients, medical treatment usually reduces intussusception without the need for surgical intervention.