bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serological assays to measure dimeric IgA antibodies in SARS‐CoV‐2 infections
Authors: Wei Zihui , Angrisano Fiona , Eriksson Emily M , Mazhari Ramin , Van Huy , Zheng Shuning , Center Rob J , Boo Irene , McMahon James , Lau Jillian , Kiernan‐Walker Nicholas , Ruybal‐Pesántez Shazia , Mueller Ivo , Robinson Leanne J , Anderson David A , Drummer Heidi E 
Published: August 2023

AbstractCurrent serological tests cannot differentiate between total immunoglobulin A (IgA) and dimeric IgA (dIgA) associated with mucosal immunity. Here, we describe two new assays, dIgA‐ELISA and dIgA‐multiplex bead assay (MBA), that utilize the preferential binding of dIgA to a chimeric form of secretory component, allowing the differentiation between dIgA and monomeric IgA. dIgA responses elicited through severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection were measured in (i) a longitudinal panel, consisting of 74 samples (n = 20 individuals) from hospitalized cases of coronavirus disease 2019 (COVID‐19); (ii) a longitudinal panel, consisting of 96 samples (n = 10 individuals) from individuals with mild COVID‐19; (iii) a cross‐sectional panel with PCR‐confirmed SARS‐CoV‐2 infection with mild COVID‐19 (n = 199) and (iv) pre–COVID‐19 samples (n = 200). The dIgA‐ELISA and dIgA‐MBA demonstrated a specificity for dIgA of 99% and 98.5%, respectively. Analysis of dIgA responses in the longitudinal panels revealed that 70% (ELISA) and 50% (MBA) of patients elicited a dIgA response by day 20 after PCR diagnosis with a SARS‐CoV‐2 infection. Individuals with mild COVID‐19 displayed increased levels of dIgA within the first 3 weeks after diagnosis but responses appeared to be short lived, compared with sustained IgA levels. However, in samples from hospitalized patients with COVID‐19 we observed high and sustained levels of dIgA, up to 245 days after PCR diagnosis. Our results suggest that severe COVID‐19 infections are associated with sustained levels of plasma dIgA compared with mild cases.
Using immunogenic cell death to improve anticancer efficacy of immune checkpoint inhibitors: From basic science to clinical application
Authors: Ghiringhelli François , Rébé Cédric 
Published: August 2023

SummaryEven though the discovery of immune checkpoint inhibitors (ICIs) has revolutionized cancer treatment, a high proportion of patients do not respond. Moreover, some types of cancers are refractory to these treatments. Thus, the need to find predictive biomarkers of efficacy and to evaluate the association with other treatments, such as chemotherapy or radiotherapy, appears to be essential. Because ICIs reactivate or maintain an active status of T cells, one possibility is to combine these treatments with therapies that engage an immune response against tumor cells. Thus, by inducing immunogenic cell death (ICD) of cancer cells, some conventional anticancer treatments induce such immune response and may have an interest to be combined with ICIs. In this review, we explore preclinical studies and clinical trials that evaluate the combination of ICIs with ICD inducers. More than inducing ICD, some of these treatments appear to modulate the tumor microenvironment and more particularly to inhibit immunosuppression, thus improving treatment efficacy.
Oestrogen treatment restores dentate gyrus development in premature newborns by IGF1 regulation
Authors: Sharma Deep R. , Cheng Bokun , Sahu Rauhin , Zhang Xusheng , Mehdizadeh Rana , Singh Divya , Iacobas Dumitru , Ballabh Praveen 
Published: August 2023

AbstractPrematurely‐born infants cared for in the neonatal units suffer from memory and learning deficits. Prematurity diminishes neurogenesis and synaptogenesis in the hippocampal dentate gyrus (DG). This dysmaturation of neurons is attributed to elevated PSD95, NMDR2A, and IGF1 levels. Since oestrogen treatment plays key roles in the development and plasticity of DG, we hypothesized that 17β‐estradiol (E2) treatment would ameliorate neurogenesis and synaptogenesis in the DG, reversing cognitive deficits in premature newborns. Additionally, E2‐induced recovery would be mediated by IGF1 signalling. These hypotheses were tested in a rabbit model of prematurity and nonmaternal care, in which premature kits were gavage‐fed and reared by laboratory personnel. We compared E2‐ and vehicle‐treated preterm kits for morphological, molecular, and behavioural parameters. We also treated kits with oestrogen degrader, RAD1901, and assessed IGF1 signalling. We found that E2 treatment increased the number of Tbr2+ and DCX+ neuronal progenitors and increased the density of glutamatergic synapses in the DG. E2 treatment restored PSD95 and NMDAR2A levels and cognitive function in preterm kits. Transcriptomic analyses showed that E2 treatment contributed to recovery by influencing interactions between IGF1R and neurodegenerative, as well as glutamatergic genes. ERα expression was reduced on completion of E2 treatment at D7, followed by D30 elevation. E2‐induced fluctuation in ERα levels was associated with a reciprocal elevation in IGF1/2 expression at D7 and reduction at D30. ERα degradation by RAD1901 treatment enhanced IGF1 levels, suggesting ERα inhibits IGF1 expression. E2 treatment alleviates the prematurity‐induced maldevelopment of DG and cognitive dysfunctions by regulating ERα and IGF1 levels.
Association between probiotic consumption and periodontitis: Evidence from NHANES 2009–2014
Authors: Ren Zhengyun , Xue Yan , Zhang Hui , Zhang Tongtong , Wang Mengyuan , Guo Tailin , Xie Jiang 
Published: August 2023

AbstractAimThe study aimed to provide evidence of the relationship between probiotics consumption and periodontitis.Materials and MethodsA total of 4577 adults who participated in the National Health and Nutrition Examination Survey (NHANES) during 2009–2014 were included in the study. The weighted prevalence of periodontitis was compared among different groups, and a weighted binary logistic regression analysis was conducted to explore the relationship between probiotic consumption and periodontitis. Receiver operating characteristic (ROC) curves were used to assess the role of probiotic consumption in the periodontitis prediction model.ResultsParticipants who consumed probiotics had a significantly lower prevalence of periodontitis than those who did not (41.08% vs. 27.83%, p < .001). After fully adjusting for all factors, the odds ratio associated with periodontitis for consuming probiotics was 0.70 (95% confidence interval 0.54–0.92, p = .01) when compared with those who did not consume probiotics. A predictive model including age, sex, ethnicity, poverty income ratio, smoking status and probiotics had 77.0% sensitivity and 60.3% specificity in detecting periodontitis in US adults and achieved an area under the ROC curve of 0.749.ConclusionsThese results indicate that consuming probiotics is associated with a reduced risk of periodontitis.
The needs of family caregivers of persons living with dementia cared for in primary care practices
Authors: Vetter Michael , Donelan Karen , Guzikowski Sophia , Michael Carie , Ritchie Christine S. , Vogeli Christine , Forester Brent P. 
Published: August 2023

AbstractRationaleThe informal caregivers who provide unpaid support for persons living with dementia (PLWD) are often unprepared to appropriately manage symptoms and navigate health services to support themselves or the PLWD.AimTo understand informal caregivers' perceived capabilities of handling dementia symptomology and perceived support from providers.MethodsWe identified and surveyed caregivers of primary care patients in the Mass General Brigham health system. We included a self‐efficacy questionnaire to assess caregivers' ability and confidence in access to dementia care, symptom management, and provider support.ResultsRespondents indicated that although their provider had knowledge of dementia and memory care, they were least likely to agree (39.2%) that their provider helped them with these challenging symptoms. Those who live with the care recipient were least likely to receive advice about common symptoms (43.6%) and to access community services (63.8%), and in general felt moderately knowledgeable about the progression of the disease (47.9%).ConclusionsThe findings imply that caregivers are aware of disease progression, dementia symptoms, and do not feel supported by their providers in managing care or accessing support services. There is opportunity to support informal caregivers in a primary care setting by appropriately uptraining providers in dementia care.
Transcriptome analysis of Fenneropenaeus merguiensis in response to Vibrio proteolyticus infection
Authors: Zhong Yunqi , He Zihao , Long Xinxin , Hou Danqing , Hu Xianye , Sun Chengbo 
Published: August 2023

AbstractIn recent years, due to the destruction of the culture environment and serious ecological pressure, especially in the process of culture, residual bait, faeces and fishery drug abuse will lead to the accumulation of harmful metabolites such as ammonia nitrogen and nitrite, and biological denitrification is the most economical and effective method to remove the single. Therefore, in this study, a nitrite removal strain XA19 was isolated and screened from a shrimp biofloc culture pond. This strain was identified as a clade of Vibrio proteolyticus because the homology between XA19 and V. proteolyticus WDVP was as high as 99.86% by using 16S rDNA gene sequence analysis and NCBI database comparison. Scanning electron microscopy images showed that V. proteolyticus is short‐rod‐shaped with a curved body and no budding spores, pods and flagella. Antimicrobial susceptibility test proved that V. proteolyticus was resistant to ampicillin, oxacillin, penicillin, vancomycin and clindamycin. In the median lethal concentration 50 (LC50) test, at 7‐day post‐infection (dpi), LC50 of V. proteolyticus for Fenneropenaeus merguiensis was 1.69 × 104 CFU/mL. Transcriptome sequencing analysis was carried out on hepatopancreas of F. merguiensis at 24 and 48 hpi. A total of 176 differentially expressed genes (DEGs) were screened at 24 hpi, including 104 up‐regulated DEGs and 72 down‐regulated DEGs, and a total of 52 DEGs were screened at 48 hpi, including 32 up‐regulated DEGs and 20 down‐regulated DEGs. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs, many immune‐related signalling pathways were significantly enriched, including Hippo signalling pathway, phagosome, Toll and Imd signalling pathways and Wnt signalling pathway. In addition, some pathways related to Warburg effect were also enriched, including Glycolysis/Gluconeogenesis, Biosynthesis of amino acids, amino sugar and nucleotide sugar metabolism and so on. In this study, the toxicity and drug sensitivity of V. proteolyticus were systematically studied, and the immune response of hepatopancreas of F. merguiensis to V. proteolyticus infection was preliminarily revealed from the molecular level. The results may provide a reference for the prevention and control of V. proteolyticus.
Identification of clinical phenotypes associated with poor prognosis in patients with nonalcoholic fatty liver disease via unsupervised machine learning
Authors: Ito Takanori , Morooka Hikaru , Takahashi Hirokazu , Fujii Hideki , Iwaki Michihiro , Hayashi Hideki , Toyoda Hidenori , Oeda Satoshi , Hyogo Hideyuki , Kawanaka Miwa , Morishita Asahiro , Munekage Kensuke , Kawata Kazuhito , Tsutsumi Tsubasa , Sawada Koji , Maeshiro Tatsuji , Tobita Hiroshi , Yoshida Yuichi , Naito Masafumi , Araki Asuka , Arakaki Shingo , Kawaguchi Takumi , Noritake Hidenao , Ono Masafumi , Masaki Tsutomu , Yasuda Satoshi , Tomita Eiichi , Yoneda Masato , Tokushige Akihiro , Ishigami Masatoshi , Kamada Yoshihiro , Ueda Shinichiro , Aishima Shinichi , Sumida Yoshio , Nakajima Atsushi , Okanoue Takeshi ,   for the Japan Study Group of Nonalcoholic Fatty Liver Disease (JSG‐NAFLD)
Published: August 2023

AbstractBackground and AimsBoth fibrosis status and body weight are important for assessing prognosis in nonalcoholic fatty liver disease (NAFLD). The aim of this study was to identify population clusters for specific clinical outcomes based on fibrosis‐4 (FIB‐4) index and body mass index (BMI) using an unsupervised machine learning method.MethodsWe conducted a multicenter study of 1335 biopsy‐proven NAFLD patients from Japan. Using the Gaussian mixture model to divide the cohort into clusters based on FIB‐4 index and BMI, we investigated prognosis for these clusters.ResultsThe cohort consisted of 223 cases (16.0%) with advanced fibrosis (F3–4) as assessed from liver biopsy. Median values of BMI and FIB‐4 index were 27.3 kg/m2 and 1.67. The patients were divided into four clusters by Bayesian information criterion, and all‐cause mortality was highest in cluster d, followed by cluster b (P = 0.001). Regarding the characteristics of each cluster, clusters d and b presented a high FIB‐4 index (median 5.23 and 2.23), cluster a presented the lowest FIB‐4 index (median 0.78), and cluster c was associated with moderate FIB‐4 level (median 1.30) and highest BMI (median 34.3 kg/m2). Clusters a and c had lower mortality rates than clusters b and d. However, all‐cause of death in clusters a and c was unrelated to liver disease.ConclusionsOur clustering approach found that the FIB‐4 index is an important predictor of mortality in NAFLD patients regardless of BMI. Additionally, non‐liver‐related diseases were identified as the causes of death in NAFLD patients with low FIB‐4 index.
IPO performance of portfolio ventures funded by impact investors versus venture capital investors
Authors: Hirschmann Mirko , Fisch Christian 
Published: August 2023

AbstractWith the global rise of impact investments to achieve societal goals, an increasing number of new ventures that aim to create societal impact now seek to go public. However, while many conceptual studies suggest that impact investors are less likely to pursue financially oriented exit plans in exchange for generating societal impact, there is little empirical evidence to back up this claim. Using a propensity score matching approach, we draw on data from Crunchbase and empirically compare the initial public offering (IPO) performance of a large sample of 3398 investments by impact investors with 3398 investments by matched venture capital (VC) investors. We find that impact investor investments are less likely to lead to an IPO than VC investments. Besides, our results indicate that syndication by impact investors increases the likelihood of an IPO. This suggests that companies funded by impact investors benefit more from syndication than their VC‐funded counterparts. Our findings have practical implications for impact investors, portfolio ventures, and policymakers.
Adverse reactions to cosmetic implants after COVID‐19 vaccination: A literature review
Authors: Cheng Wen‐jie , Cai Zi‐xing , Tang Xiao‐jun 
Published: August 2023

AbstractBackgroundAs the world's population of people vaccinated with the COVID‐19 vaccine increases, adverse reactions are increasingly being reported. There have been progressive reports of the effects of COVID‐19 vaccination on cosmetic fillers or prostheses, but they have not been reviewed based on their clinical morphologic patterns. This article reviewed the progress of research on adverse reactions to cosmetic implants after COVID‐19 vaccination.MethodsWe researched the English‐language literature up to October 15, 2022, using predefined keywords to identify relevant studies about adverse reactions to cosmetic implants after the COVID‐19 vaccination, collecting patient characteristics, implant type, the time interval between vaccination and implantation or injection, time of onset, symptoms, treatments, and outcomes.ResultsAmong the adverse reactions to implants associated with COVID‐19 vaccination, we distinguished between (1) injectable fillers and (2) surgical prosthetic implants. The most common adverse reactions were at the site of hyaluronic acid injection and breast prosthesis after Pfizer vaccination, mainly DIRs, and mainly manifested as edema, rash, fever, and capsular contracture. This paper also reported the possible causes, treatments of DIRs, and limitations of current studies.ConclusionsIn this article, we attempted to investigate and discuss all the adverse reactions of cosmetic implants related to COVID‐19 vaccination in the current literature, to unmask these reactions and make a more accurate assessment of vaccine safety.
Cosmetic therapies for Chinese patients with acne taking concomitant or recent intake of oral isotretinoin: A retrospective study
Authors: Qin Xiaolei , Huang Haiyan , Zou Yanfen , Yu Bo , Tu Ping 
Published: August 2023

AbstractBackgroundThe current standard recommendation is to initiate the cosmetic therapies after discontinuing taking oral isotretinoin for at least 6 months. However, this recommendation has been questioned in several recent publications, and it is difficult to operate in clinical practice as early initiation of effective treatment is desirable for patients with acne sequelae.ObjectiveThe purpose of this study is to evaluate the efficacy and safety of chemical peeling and light/laser or radiofrequency treatments combined with oral isotretinoin for patients with acne vulgaris and acne scars.MethodA retrospective study of 511 patients on/or recently administered with isotretinoin treated with glycolic acid, intense pulsed light, nonablative fractional laser, fractional radiofrequency, and ablative carbon dioxide laser. A total of 1352 interventions were performed. The medical follow‐up lasted for at least 1 year. The efficacy and safety of different procedures were evaluated.ResultsA total of 511 patients, who were treated with isotretinoin orally or stopped for <6 months, received 477 sessions of glycolic acid chemical peeling treatment, 588 sessions of intense pulsed light treatment, 61 sessions of nonablative fractional laser treatment, 101 sessions of fractional radiofrequency treatment, and 125 sessions of ablative fractional carbon dioxide laser treatment. No hypertrophic scars and keloids were found, and the incidence of serious adverse reactions such as scarring, erythema, blisters, and postinflammatory hyperpigmentation did not increase.ConclusionsIt is safe to perform skin procedures in patients with acne and acne scars during or after discontinuation of isotretinoin for <6 months. Invasive treatments such as ablative fractional carbon dioxide laser treatment can be performed, as appropriate, by an experienced physician. The guideline of avoiding chemical and physical procedures in such patients taking oral isotretinoin should to revised.
Molecular biological analysis revealed a case of synchronous endometrial and ovarian cancer with different histological grade as metastatic ovarian cancer from endometrial cancer: Case report and review of literature
Authors: Yumisashi Riho , Saito Ryosuke , Togami Shinichi , Kobayashi Yusuke , Kitazono Ikumi , Tanimoto Akihide , Kobayashi Hiroaki 
Published: August 2023

AbstractThe diagnosis of synchronous endometrial and ovarian cancer or metastatic cancer of the same histological type is difficult. In this study, molecular biology analysis was performed to determine ovarian metastasis from endometrial cancer. A 38‐year‐old woman had pathological evidence of endometrial cancer (endometrioid carcinoma, grade 1) and ovarian cancer (endometrioid carcinoma, grade 3); a disseminated nodule in the serosa uteri was also diagnosed as endometrioid carcinoma (grade 3). Customized panel sequencing revealed a common mutation pattern in ovarian cancer and disseminated nodules. Furthermore, endometrial cancer showed the same mutation patterns for FGFR3 and PTEN as ovarian cancer and disseminated nodules. All tumors were microsatellite instability high. Clinicopathological and molecular biology analyses suggested that the patient had ovarian metastasis from endometrial cancer. The patient underwent adjuvant chemotherapy with paclitaxel and carboplatin, with no recurrence. Molecular biology techniques may enable appropriate treatment based on clinically accurate diagnosis.
Seasonal upwelling conditions promote growth and calcification in reef‐building coralline algae
Authors: Pulecio‐Plaza Lauri , Diaz‐Pulido Guillermo , García‐Urueña Rocío 
Published: August 2023

AbstractCrustose coralline algae (CCA) are important components of reef ecology contributing to reef framework construction. However, little is known about how seasonal upwelling systems influence growth and calcification of tropical CCA. We assessed marginal and vertical growth and net calcification rates of two dominant but morphologically different reef‐building CCA,Porolithon antillarumandLithophyllumcf.kaiseri, in a shallow coral reef of the Colombian Caribbean during upwelling and non‐upwelling seasons. Growth and calcification rates varied seasonally with higher values during the upwelling compared to the non‐upwelling (rainy) season. Annual vertical growth showed rates of 4.48 ± 1.58 and 4.31 ± 2.17 mm · y−1, net calcification using crust growth estimates of 0.75 ± 0.30 g and 0.68 ± 0.60 g CaCO3 · cm−2 · y−1and net calcification using the buoyant weight method of 1.49 ± 0.57 and 0.52 ± 0.11 g CaCO3 · cm−2 · y−1inP. antillarumandL. kaiseri, respectively. Seawater temperature was inversely related with growth and calcification; however, complex oceanographic interactions between temperature and resource availability (e.g., light, nutrients, and CO2) are proposed to modulate CCA vital rates. Although CCA calcification rates are comparable to hard corals, CCA vertical accretion is much lower, suggesting that the main contribution of CCA to reef construction is via cementation processes. These results provide baseline data on CCA in the region and generate useful information for monitoring the impacts of environmental changes on tropical upwelling environments.
Binge drinking episode causes acute, specific alterations in systemic and hepatic inflammation‐related markers
Authors: Stankevic Evelina , Israelsen Mads , Juel Helene Bæk , Madsen Anne Lundager , Ängquist Lars , Aldiss Peter Stuart Jacob , Torp Nikolaj , Johansen Stine , Hansen Camilla Dalby , Hansen Johanne Kragh , Thorhauge Katrine Holtz , Lindvig Katrine Prier , Madsen Bjørn Stæhr , Sulek Karolina , Legido‐Quigley Cristina , Thiele Maja Sofie , Krag Aleksander , Hansen Torben 
Published: August 2023

AbstractBackgroundFrequent binge drinking is a known contributor to alcohol‐related harm, but its impact on systemic and hepatic inflammation is not fully understood. We hypothesize that changes in immune markers play a central role in adverse effects of acute alcohol intake, especially in patients with early liver disease.AimTo investigate the effects of acute alcohol intoxication on inflammation‐related markers in hepatic and systemic venous plasma in people with alcohol‐related liver disease (ArLD), non‐alcoholic fatty liver disease (NAFLD) and healthy controls.MethodsThirty‐eight participants (13 with ArLD, 15 with NAFLD and 10 healthy controls) received 2.5 mL of 40% ethanol per kg body weight via a nasogastric tube. Seventy‐two inflammation‐related markers were quantified in plasma from hepatic and systemic venous blood, at baseline, 60 and 180 min after intervention.ResultsAlcohol intervention altered the levels of 31 of 72 and 14 of 72 markers in the systemic and hepatic circulation. All changes observed in the hepatic circulation were also identified in the systemic circulation after 180 min. Only FGF21 and IL6 were increased after alcohol intervention, while the remaining 29 markers decreased. Differences in response to acute alcohol between the groups were observed for 8 markers, and FGF21 response was blunted in individuals with steatosis.ConclusionAcute alcohol intoxication induced changes in multiple inflammation‐related markers, implicated in alcohol metabolism and hepatocellular damage. Differences identified between marker response to binge drinking in ArLD, NAFLD and healthy controls may provide important clues to disease mechanisms and potential targets for treatment.Clinical trial number: NCT03018990