bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis
Authors: Zhang Ning , Jiang Yue , Sun Yun-Juan , Jiang Jian-Chun , Tong Ya-Juan 
Published: January 2023

Introduction: Hemicellulose is an important component in lignocellulose materials, which is second only to cellulose, accounting for 15%–35% of the dry weight of plants. In the current situation of energy shortage, making full use of lignocellulose materials to produce fuel ethanol has become an important way to solve the energy problem. Xylanase plays a crucial role in the utilization of hemicellulose. It is a necessary means to reduce the cost of hemicellulose utilization by improving the activity of xylanase. Moreover, most naturally xylanases are mesophilic enzymes, which limits their industrial application.Methods:In this study, Myceliophthora thermophila was used to produce xylanases and a thermostable mutant M 2103 was obtained by atmospheric room temperature plasma (ARTP) mutagenesis. The research work started with exploring the effects of ARTP mutagenesis on the antioxidase system [superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO), and antioxidant capacity (AOC)] of M. thermophile, and found that superoxide dismutase activity increased by 221.13%, and polyphenol oxidase activity increased by 486.04% as compared with the original strain when the implantation time was 300 s. So as to determine the conditions for subsequent mutagenesis.Results and Discussion:For the mutant M 2103, the reaction temperature for xylanase production remained stable in the range of 70°C–85°C. Its optimum temperature was 75°C, which was 15°C higher than that of the original strain. And its xylanase activity increased by 21.71% as compared with the original strain. M 2103 displayed a significantly higher relative xylanase activity than the original strain in the acidic (pH 4.0–7.0) range, and the xylanase activity was relatively stable in the pH range of 6.0–8.5. These results provide an alternative biocatalyst for the production of xylooligosaccharide, and a potential usage of ARTP in the mutagenesis of thermostable mutant.
Rapid detection of influenza A viruses using a real-time reverse transcription recombinase-aided amplification assay
Authors: Cui Huan , Zhang Cheng , Tu Fei , Zhao Kui , Kong Yunyi , Pu Jie , Zhang Lei , Chen Zhaoliang , Sun Yuanyuan , Wei Yujie , Liang Chuncai , Liu Juxiang , Liu Jun , Guo Zhendong 
Published: January 2023

IntroductionInfluenza A viruses (IAVs) are important pathogens of respiratory infections, causing not only seasonal influenza but also influenza pandemics and posing a global threat to public health. IAVs infection spreads rapidly, widely, and across species, causing huge losses, especially zoonotic IAVs infections that are more harmful. Fast and sensitive detection of IAVs is critical for controlling the spread of this disease.MethodsHere, a real-time reverse transcription recombinase-aided amplification (real-time RT-RAA) assay targeting conserved positions in the matrix protein gene (M gene) of IAVs, is successfully established to detect IAVs. The assay can be completed within 20 min at 42°C.ResultsThe sensitivity of the real-time RT-RAA assay was 142 copies per reaction at 95% probability, which was comparable to the sensitivity of the RT-qPCR assay. The specificity assay showed that the real-time RT-RAA assay was specific to IAVs, and there was no cross-reactivity with other important viruses. In addition, 100%concordance between the real-time RT-RAA and RT-qPCR assays was achieved after testing 120 clinical specimens.DiscussionThe results suggested that the real-time RT-RAA assay we developed was a specific, sensitive and reliable diagnostic tool for the rapid detection of IAVs.
Impact of different guidewires on the implantation depth using the largest self-expandable TAVI device
Authors: Veulemans Verena , Wilde Nihal , Wienemann Hendrik , Adrichem Rik , Hokken Thijmen W. , Al-Kassou Baravan , Shamekhi Jasmin , Mauri Victor , Maier Oliver , Jung Christian , Horn Patrick , Adam Matti , Nickenig Georg , Baldus Stephan , Van Mieghem Nicolas M. , Kelm Malte , Sedaghat Alexander , Zeus Tobias 
Published: January 2023

BackgroundThe deployment process of the largest self-expandable device (STHV-34) during transcatheter aortic valve implantation (TAVI) might be challenging due to stabilization issues. Whether the use of different TAVI-guidewires impact the procedural success and outcome is not well-known. Therefore, we sought to evaluate the impact of non-Lunderquist (NLu) vs. the Lunderquist (Lu) guidewires during TAVI using the STHV-34 on the procedural and 30-day outcomes.MethodsThe primary study endpoint was defined as the final implantation depth (ID) depending on the selected guidewire strategy. Key secondary endpoints included VARC-3-defined complications.ResultsThe study cohort included 398 patients of four tertiary care institutions, of whom 79.6% (317/398) had undergone TAVI using NLu and 20.4% (81/398) using Lu guidewires. Baseline characteristics did not substantially differ between NLu and Lu patients. The average ID was higher in the Lu cohort (NLu vs. Lu: −5.2 [−7.0–(−3.5)] vs. −4.5 [−6.0–(−3.0)]; p = 0.022*). The optimal ID was reached in 45.0% of patients according to former and only in 20.1% according to nowadays best practice recommendations. There was no impact of the guidewire use on the 30-day outcomes, including conduction disturbances and pacemaker need (NLu vs. Lu: 15.1 vs. 18.5%; p = 0.706).ConclusionThe use of the LunderquistTM guidewire was associated with a higher ID during TAVI with the STHV-34 without measurable benefits in the 30-day course concerning conduction disturbances and associated pacemaker need. Whether using different guidewires might impact the outcome in challenging anatomies should be further investigated in randomized studies under standardized conditions.
CMR detects decreased myocardial deformation in asymptomatic patients at risk for heart failure
Authors: Hashemi Djawid , Doeblin Patrick , Blum Moritz , Weiss Karl Jakob , Schneider Matthias , Korosoglou Grigorios , Beyer Rebecca Elisabeth , Pieske Burkert , Edelmann Frank , Kelle Sebastian 
Published: January 2023

AimsThe main management strategy of heart failure with preserved ejection fraction (HFpEF) is prevention since HFpEF is associated with many cardiovascular (CV) risk factors, especially since HFpEF is linked to a high risk for both mortality and recurrent heart failure (HF) hospitalizations. Therefore, there is a need for new tools to identify patients with a high risk profile early. Regional strain assessment by CMR seems to be superior in describing deformation impairment in HF. The MyoHealth score is a promising tool to identify cardiac changes early.Methods and resultsHeart failure patients irrespective of LVEF and asymptomatic controls were recruited, and CMR based measures were obtained. For this analysis the asymptomatic control group (n = 19) was divided into asymptomatic subjects without CV co-morbidities or evidence of cardiac abnormalities and (n = 12) and asymptomatic subjects with CV co-morbidities or evidence of cardiac abnormalities (n = 7) as well as patients with HFpEF (n = 19). We performed CMR scans at rest and during a stress test using isometric handgrip exercise (HG). Assessing the MyoHealth score at rest revealed preserved regional strain in 85 ± 9% of LV segments in controls, 73 ± 11% in at Risk subjects and 73 ± 8% in HFpEF patients. During stress the MyoHealth score was 84 ± 7% in controls, 83 ± 7 in at risk subjects and 74 ± 11 in HFpEF patients.ConclusionIn summary, we show for the first time that asymptomatic subjects with increased CV risk present with HFpEF like impaired myocardial deformation at rest, while they show results like controls under HG stress. The potential of preventive treatment in this group of patients merits further investigation in future.Clinical trial registration[https://drks.de/search/de/trial/DRKS00015615], identifier [DRKS00015615].
An Olive Oil Mill Wastewater Extract Improves Chemotherapeutic Activity Against Breast Cancer Cells While Protecting From Cardiotoxicity
Authors: Benedetto Nadia , Calabrone Luana , Gutmańska Karolina , Macrì Nicoletta , Cerrito Maria Grazia , Ricotta Riccardo , Pelosi Giuseppe , Bruno Antonino , Noonan Douglas M. , Albini Adriana 
Published: January 2023

Graphical AbstractThe cartoon summarized the major approaches and insights of the manuscript: (A) the possibility to recover waste material from extra-virgin olive oil (EVOO) processing that allows the repurposing of polyphenol-rich extracts characterized by (B) antiangiogenic activities in vivo, antiproliferative activities in vitro on BC cell lines (C), cardioprotective activities on rat and human cardiomyocytes (HCMs) (D), and in vivo on the zebrafish embryo heart (E).
Influence radius of pressure relief of energy-accumulating blasting and water injection in the heading face of the liangbei coal mine
Authors: Wang Zhenfeng , Wang Yu , Wang Feng 
Published: January 2023

This study aims to reasonably determine the influence radius of pressure relief of energy-accumulating blasting and water injection in the Liangbei Coal Mine and effectively eliminate local stress concentration and coal seam outburst risk. The change in gas parameters in each test hole before and after energy-accumulating blasting and water injection is determined through ANSYS/LSDYNA numerical simulation, theoretical analysis and calculation, and the application of the gas parameter method in the 32051 heading face of the Liangbei Coal Mine. The influence radius of pressure relief technology of energy-accumulating blasting and water injection in the Liangbei Coal Mine in the direction of energy-accumulating trough is studied, which provides reliable data for the layout parameters of energy-accumulating blasting and water injection hole in the working face of the Liangbei Coal Mine. The experimental results are as follows: 1) The simulation via ANSYS/LSDYNA shows that a blasting cavity is formed by an explosive shock wave in the borehole. The diameter of the blasting cavity in the energy-accumulating direction is 42 cm, which is 10 times the diameter of the energy-accumulating blasting and water injection hole. The diameter of the blasting cavity in the non-energy-accumulating direction is 14 cm, which is 3.3 times the diameter of the energy-accumulating blasting and water injection hole. The stress in the energy-accumulating direction is 2.9 times larger than that in the non-energy-accumulating direction. The crack length in the energy-accumulating direction is 294 cm, and the crack length in the non-energy-accumulating direction is 80 cm. 2) Theoretical analysis and calculation present that the crushing area of shaped charge blasting is 39 cm, the fracture area is 267 cm, and the vibration area is 159 cm. 3) Comparison of the change in gas parameters of each test hole in the field experiment before and after energy-accumulating blasting and water injection indicates that the influence radius of pressure relief in the direction of energy-accumulating trough is 300 cm. 4) The layout of energy-accumulating blasting and water injection in the working face of the Liangbei Coal Mine with 550 cm borehole spacing demonstrates that the verification index of gas drilling decreases, the analytical values (Δh2) decrease from 120–140 Pa to 100–120 Pa, the drilling cutting weight (S) decreases from 3.2 to 4.6 kg/m to 3.0–3.8 kg/m, and stress is released and transferred to deep coal. This study has guiding significance for outburst elimination by local arrangement of energy-gathering blasting and water injection borehole in the working face of the Liangbei Coal Mine.
Hydrocarbon accumulation conditions and favorable exploration zones in the Lunpola Basin, Tibet
Authors: Zhang Yunbo , Wang Rui , Xu Jie , Wu Xinhe , Li Yinglie , Lin Yanhua , Wang Buqing 
Published: January 2023

By systematically reviewing and summarizing the previous studies on the petroleum geology of the Lunpola Basin, combined with the latest oil and gas exploration results, the oil and gas accumulation conditions of this basin are summarized, and the favorable zones with the most exploration potential are pointed out. The results show that: ① The Paleogene mainly develops three sets of source rocks, including Niu-1 section, Niu-2 section and lower Niu-3 subsection. The lithology is mainly semi-deep and deep lacustrine gray-dark shale. Among them, the source rock of Niu-2 section is the best. However, the source rock of Niu-1 section is only developed in the Jiangjiacuo depression. The effective source rocks have the largest thickness in the central and western parts of the basin and have the best hydrocarbon-generating capacity. The thickness and abundance of the source rocks have decreased eastward. The organic matter are mainly Type I and Type II1, with a maturity of 0.58%–1.08%. ② The study area is dominated by clastic rock reservoirs, which can be divided into three types according to the strata distribution, and the overall reservoir conditions are not good. The reservoir porosity is between 0.1% and 32.6%, mainly between 2.0% and 10.0%, with an average of 6.5%; the permeability is between 0.0005 mD and 116.0 mD, mainly between 0.01 mD and 0.5 mD, which means ultra-low porosity and ultra-low permeable fracture-porous reservoirs. ③ Three sets of seals are mainly developed in the Lunpola Basin, including two regional seals of the middle Niu-2 subsection and Dingqinghu section, and a direct seal of upper Niu-2 subsection. These three sets of seals are well developed and have good preservation conditions in the relatively stable central depression and the footwall of thrust nappe structural belt . ④ The oil and gas reservoirs in the Lunpola Basin have the distribution law of “structural traps in the north and south, and lithologic traps in the center”. Structural traps are mainly distributed in the thrust nappe belt in the north and the Changshan uplift in the south, forming “self-generation and self-storage” or “lower-generation and upper-storage” reservoirs. While lithologic traps are mainly distributed in the central sag, forming “self-generation and self-storage” reservoirs. ⑤ The source-reservoir-caprock combination conditions are good in the structural traps in the footwall of the northern thrust nappe belt and the lithologic traps in the southern slope belt of Jianjiacuo depression,, and they are the most favorable oil and gas exploration zones in the Lunpola Basin.
Association of bone mineral density with prediabetes risk among African-American and European-American adult offspring of parents with type 2 diabetes
Authors: Liu Zhao , Asuzu Peace , Patel Avnisha , Wan Jim , Dagogo-Jack Sam 
Published: January 2023

IntroductionType 2 diabetes mellitus (T2DM) is associated with alterations in bone mineral density (BMD), but association between prediabetes and BMD is unclear.MethodsWe analyzed BMD among the initially normoglycemic participants in the Pathobiology of Prediabetes in a Biracial Cohort (POP-ABC) study in relation to incident prediabetes during 5 years of follow-up.Results and DiscussionA total of 343 participants (193 Black, 150 White) underwent DEXA during Year 1 of POP-ABC and were followed quarterly for 5 years. The mean age was 44.2 ± 10.6 years; BMI was 30.2 ± 7.23 kg/m2. At baseline, the mean BMD was 1.176 ± 0.135 g/cm2 (1.230 ± 0.124 g/cm2 in men vs. 1.154 ± 0.134 g/cm2 in women, P&lt;0.0001; 1.203 ± 0.114 g/cm2 in Black vs. 1.146 ± 0.150 g/cm2 in White participants, P=0.0003). During 5 years of follow-up, 101 participants developed prediabetes and 10 subjects developed T2DM (progressors); 232 were nonprogressors. Progressors to prediabetes had numerically higher baseline BMD and experienced lower 1-year decline in BMD (P&lt;0.0001) compared with nonprogressors. From Kaplan-Meier analysis, the time to 50% prediabetes survival was 2.15 y among participants in the lowest quartile of baseline BMD, longer than those in higher quartiles (1.31 – 1.41 y). Values for BMD correlated inversely with age and adiponectin levels, and positively with BMI. In logistic regression analysis, BMD z score significantly predicted incident prediabetes: more negative BMD z scores were associated with decreased incident prediabetes (odds ratio 0.598 [95% confidence interval 0.407 - 0.877], P=0.0085), after controlling for age, BMI, change in BMI, ethnicity, blood glucose and adiponectin.ConclusionsAmong initially normoglycemic individuals, higher baseline BMD was associated with higher risk of incident prediabetes during 5 years of follow-up.
Association between transferred embryos and multiple pregnancy/live birth rate in frozen embryo transfer cycles: A retrospective study
Authors: Wu Xian , Zhou Wen-jie , Xu Bu-fang , Chen Qian , Xia Lan , Zhao Shen , Xu Hui-hui , Zhang Ai-jun , Niu Zhi-hong 
Published: January 2023

BackgroundPhysicians need an appropriate embryo transfer strategy to address the challenge of reducing multiple birth rates, while maintaining the couples’ live birth rate during assisted reproductive technology.MethodsWe included 10,060 frozen embryo transfer cycles from January 2015 to March 2020 in reproductive medical center of Ruijin hospital, Shanghai, China. Patients were grouped according to the number and grade of cleavage-stage embryo or blastocysts transferred. Live birth rate and multiple live birth rate were compared among groups of women of different ages. Multivariable logistic regression models were used to estimate the risk of multiple live birth using different combinations of transferred embryos.ResultsThe transfer of double good-quality embryos was an independent predictor for multiple birth in women aged &lt;30 years and those aged 36−39 years [&lt;30 years: aOR =1.54 (95% CI: 1.14−2.06, P &lt; 0.01); 36−39 years: aOR =1.84 (95% CI: 1.0−3.4, P &lt; 0.01)]. Further, for women aged &lt;36 years, the transfer of good-quality + poor-quality blastocysts was an independent predictor for multiple birth rate [&lt;30 years: aOR=2.46 (95% CI: 1.45−4.18, P &lt; 0.01); 31−35 years: aOR =4.45 (95% CI: 1.97−10.06, P &lt; 0.01)].ConclusionsSingle-good-quality blastocyst transfer is recommended for women of all ages. When good-quality cleavage embryos are available, the choice of single or double embryo transfer with good- or average-quality embryo should depend on the age of women. Double embryo transfer with the highest possible grade of embryos is recommended for women aged ≥40 years.
Survival association of XRCC1 for patients with head and neck squamous cell carcinoma: A systematic review and meta-analysis
Authors: Yang Fan , Zhou Liuqing , Chen Jingcai , Luo Yao , Wang Yanjun 
Published: January 2023

Background: Epidemiologic studies have demonstrated that X-ray repair cross-complementary group 1 (XRCC1) is one of the susceptibility factors in head and neck squamous cell carcinoma (HNSCC) patients. However, its clinical prognostic impact remains controversial. Thus, a meta-analysis was performed to clarify the association between XRCC1 and the survival outcomes in HNSCC patients.Methods: Following the Preferred Reporting Items or Systematic Reviews Meta Analyses (PRISMA) 2020 guidelines, literature searches were systematically performed in PubMed, EMBASE, Web of Science, Wanfang, and Chinese National Knowledge Infrastructure (CNKI) databases with manual retrieval. Hazard ratios (HRs) and 95% confidence intervals (CIs) were collected to estimate the correlation between XRCC1 and the survival outcomes of HNSCC patients.Results: Ten studies including 1995 HNSCC patients who satisfied the inclusion and exclusion criteria were included in this meta-analysis. Pooled analysis indicated that XRCC1 Arg399Gln and XRCC1 high protein expression were significantly correlated with poor overall survival with HR of 1.31 (95% CIs: 1.03-1.66, p = 0.027) and 2.32 (95% CIs: 1.55-3.48 p = 0.000) in HNSCC patients. In addition, our results demonstrated that XRCC1 was significantly associated with poor progression-free survival (HR = 1.42, 95% CIs: 1.15-1.75, p = 0.001) in HNSCC patients.ConclusionThis meta-analysis demonstrated that XRCC1 Arg399Gln and XRCC1 high protein expression increase the risk of poor survival for HNSCC patients. XRCC1 is a potential therapeutic target for HNSCC.
Supervised learning techniques for dairy cattle body weight prediction from 3D digital images
Authors: Gebreyesus Grum , Milkevych Viktor , Lassen Jan , Sahana Goutam 
Published: January 2023

Introduction: The use of automation and sensor-based systems in livestock production allows monitoring of individual cows in real-time and provides the possibility of early warning systems to take necessary management actions against possible anomalies. Among the different RT monitoring parameters, body weight (BW) plays an important role in tracking the productivity and health status.Methods: In this study, various supervised learning techniques representing different families of methods in the machine learning space were implemented and compared for performance in the prediction of body weight from 3D image data in dairy cows. A total of 83,011 records of contour data from 3D images and body weight measurements taken from a total of 914 Danish Holstein and Jersey cows from 3 different herds were used for the predictions. Various metrics including Pearson’s correlation coefficient (r), the root mean squared error (RMSE), and the mean absolute percentage error (MAPE) were used for robust evaluation of the various supervised techniques and to facilitate comparison with other studies. Prediction was undertaken separately within each breed and subsequently in a combined multi-breed dataset.Results and discussion: Despite differences in predictive performance across the different supervised learning techniques and datasets (breeds), our results indicate reasonable prediction accuracies with mean correlation coefficient (r) as high as 0.94 and MAPE and RMSE as low as 4.0 % and 33.0 (kg), respectively. In comparison to the within-breed analyses (Jersey, Holstein), prediction using the combined multi-breed data set resulted in higher predictive performance in terms of high correlation coefficient and low MAPE. Additional tests showed that the improvement in predictive performance is mainly due to increase in data size from combining data rather than the multi-breed nature of the combined data. Of the different supervised learning techniques implemented, the tree-based group of supervised learning techniques (Catboost, AdaBoost, random forest) resulted in the highest prediction performance in all the metrics used to evaluate technique performance. Reported prediction errors in our study (RMSE and MAPE) are one of the lowest in the literature for prediction of BW using image data in dairy cattle, highlighting the promising predictive value of contour data from 3D images for BW in dairy cows under commercial farm conditions.