bluuebunny/crossref_metadata_2025_split huggingface dataset:
The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration
Authors: Corre Marie-Hélène , Rey Benjamin , David Shannon C. , Torii Shotaro , Chiappe Diego , Kohn Tamar 
Published: August 2023

SummaryEnteroviruses are human pathogens known to challenge water safety1,2. Among the microbial stressors found in water, bacterial serine proteases contribute to the control of enterovirus persistence3. However, the structural interactions accounting for the susceptibility of enteroviruses to proteases remains unexplained. Here, we describe the molecular mechanisms involved in the recruitment of serine proteases to viral capsids. Among the virus types used, coxsackievirus A9 (CVA9), but not CVB5 and echovirus 11 (E11), was inactivated by Subtilisin A in a host-independent manner, while Bovine Pancreatic Trypsin (BPT) only reduced CVA9 infectivity in a host-dependent manner. Predictive interaction models of each protease with capsid protomers indicate the main targets as internal disordered protein (IDP) segments exposed either on the 5-fold vertex (DE loop VP1) or at the 5/2-fold intersection (C-terminal end VP1) of viral capsids. We further show that a functional binding protease/capsid depends on both the strength and the evolution over time of protease-VP1 complexes, and lastly on the local adaptation of proteases on surrounding viral regions. Finally, we identified three residues on CVA9 capsid that trigger cleavage by Subtilisin A, one of which acts as a sensor residue contributing to enzyme recognition on the DE loop. Overall, this study describes an important biological mechanism involved in enteroviruses biocontrol.
PQS and Pyochelin inPseudomonas aeruginosaShare Inner Membrane Transporters to Mediate Iron Uptake
Authors: Zhang Heng , Yang Jianshe , Cheng Juanli , Zeng Jing , Ma Xin , Lin Jinshui 
Published: August 2023

ABSTRACTBacteria uptake different forms of iron through various channels to meet their needs. Our previous studies have shown that TseF, a type VI secretion system effector for Fe uptake, facilitates the delivery of outer membrane vesicle (OMV)-associated PQS-Fe3+to bacterial cells by involving the Fe(III) pyochelin receptor FptA and the porin OprF. However, the form in which the PQS-Fe3+complex enters the periplasm and how it is taken up into the cytoplasm remain unclear. Here, we first demonstrate that the PQS-Fe3+complex enters the cell directly through FptA or OprF. Next, we find that inner membrane transporters such as FptX, PchHI, and FepBCDG are not only necessary forP. aeruginosato uptake PQS-Fe3+and PCH-Fe3+, but also necessary for the virulence ofP. aeruginosatowardGalleria mellonellalarvae. Furthermore, we suggest that the function of PQS-Fe3+(but not PQS)-mediated quorum-sensing regulation is dependent on FptX, PchHI, and FepBCDG. Additionally, the findings indicate that, unlike FptX, both FepBCDG and PchHI play no role in the autoregulatory loop involving PchR, but further deletion offepBCDGandpchHIcan reverse the inactive PchR phenotype caused byfptXdeletion and reactivate the expression of the PCH pathway genes under iron-limited conditions. Finally, this work detected the interaction between FptX, PchHI, and FepBCDG, indicating that a larger complex could be formed to mediate uptake of PQS-Fe3+and PCH-Fe3+. These results pave the way for a better understanding of the PQS and PCH iron uptake pathway, and provide future directions to tackleP. aeruginosainfections.IMPORTANCEIron is a key factor forP. aeruginosato break through the host’s defense system and successfully infect. To acquire the necessary iron from the host,P. aeruginosahas evolved a number of strategies, the most common being the synthesis, secretion, and uptake of siderophores such as pyoverdine, pyochelin, and the quorum-sensing signaling molecule PQS. However, despite intensive studies of the siderophore uptake pathways ofP. aeruginosa, our understanding of how siderophores transport iron across the inner membrane into the cytoplasm is still far from complete. Here, we reveal that PQS and pyochelin inP. aeruginosashare inner membrane transporters such as FptX, PchHI and FepBCDG to mediate iron uptake. Meanwhile, PQS and pyochelin-mediated signaling operates to a large extent via these inner membrane transporters. Our study revealed an interesting phenomenon of shared uptake pathways between PQS and pyochelin, which will lead us to reexamine the role of these two molecules in the iron uptake and virulence ofP. aeruginosa.
Audit committees in public companies listed on the Warsaw Stock Exchange: compliance with hard law in practice
Authors: Gad Jacek 
Published: August 2023

PurposeThe article aims to determine the implementation extent of the regulations around appointment and characteristics of audit committees and regulations concerning disclosure of information about the audit committee in Polish practice.Design/methodology/approachThe author analyzed the informative content of management reports and corporate governance statements. The survey covered all domestic companies listed on the Warsaw Stock Exchange in the years from 2017 to 2021.FindingsThe new guidelines resulting from hard law had a significant impact on the corporate governance on the Polish capital market. According to the research results, over the analyzed years, the share of companies listed on the Warsaw Stock Exchange, which appointed an audit committee within the supervisory board, clearly increased. Moreover, the research found that in the period under study, not all companies fulfilled the obligation to disclose information about the audit committee resulting from hard law. In particular, this applies to disclosures on how the members of the audit committee acquired competencies in the area of accounting.Practical implicationsThe article concerns the operation of the audit committee in public companies listed on the Polish capital market. The study can serve as a reference point for further research on corporate governance. The results of the research may be an indication for those who create legal solutions in the area of corporate governance.Originality/valueThis is the first such comprehensive study on the characteristics of the audit committee and disclosures about the audit committee resulting from the introduction of hard law in this area.
How ambidextrous leadership enhances employee creativity: a quantitative approach
Authors: Yasmeen Aiza , Ajmal Saran Khan 
Published: August 2023

PurposeBased on the leader-members exchange (LMX) theory, the purpose of this paper is to examine the impact of ambidextrous leadership on employee creativity through ambidextrous behavior. The study also investigates an organizational culture that amplifies the positive influence of ambidextrous leadership on employee creativity.Design/methodology/approachPrimary data were collected form 300 permanent employees of the local government department of Azad Jammu and Kashmir (AJK). Hypotheses were tested by using Hayes PROCESS macro.FindingsResults indicated that ambidextrous style of leadership and employee creativity is mediated by ambidextrous behavior of employees. The findings of the study also established the fact that the relationship of ambidextrous leadership and employee creativity is subject to the organizational culture.Research limitations/implicationsThe current study was carried out in the local government department; researchers can conduct research by studying multiple government departments at once. In addition, the present study is cross-sectional; scholars in future can take a longitudinal approach to capture employee creativity at different points of time.Practical implicationsFor establishing a welcoming innovation atmosphere and assist workers' creative behaviors, ambidextrous leadership should be practiced by leaders from day-to-day operations. Leaders must create flexible abilities to adapt internal resources (knowledge and values) in the public sector into new behavioral patterns that encourage employee creativity.Originality/valueThis is the first study that investigates the ambidextrous behavior as a vital intervening mechanism in the ambidextrous leadership–employee creativity linkage. Further, this study provides first empirical evidence by study the organizational culture as a moderator in determine the impact of ambidextrous leadership on employee creativity.
Assessing the impact of COVID-19 on architectural education: a case study of Jordanian Universities
Authors: Alhusban Ahmad A. , Alhusban Safa A. , Alhusban Mohammad-Ward A. 
Published: August 2023

PurposeThis research aimed to define the factors that may impact the effectiveness of online architectural education during COVID-19 and to examine the degree of students' and instructors' satisfaction with these factors among Jordanian governmental universities. Further, the research examines the relationships/interrelationships between the degree of their satisfaction with these factors and the students' and instructors' age, gender, education level, home size, and family size.Design/methodology/approachDifferent research methods were used to achieve the research purposes, including semi-structured interviews, online questionnaires and reviewing recent literature. This research used descriptive statistics and Pearson product-moment correlation coefficient (Pearson r) to analyze the data.FindingsThis research found that online architectural education during COVID-19 was stressful. It was ineffective in teaching practical architectural courses like design and negatively affected the architectural learning process and outcomes. In contrast, the online teaching of architectural theoretical courses was reliable and convenient if the students and instructors had intentional, sufficient technological and appropriate resources. Online architectural education negatively affected the students' design ability and skills, peer review, intended learning outcomes (ILOs) achievements, the quality of feedback, workload, interaction mode, participation, collaboration, productivity and increased cheating on online exams. The home environment was unsuitable for online architectural education. Family interventions, privacy, home size and family size significantly influence online architectural education's effectiveness.Originality/valueStudents' and instructors' satisfaction determines the continuity of using online teaching mode, which depends on information quality, system quality, service quality, perceived ease of use, perceived usefulness, achieved intended learning outcomes, cognitive absorption, skills, motivation, engagement, implementing resources and strategies and positive emotions as hope and enjoyment. Students' and instructors' satisfaction reflects how they view their learning experience, which is crucial in assessing the effectiveness of online education quality that focuses on the context, input, process and product, which is still not clearly understood, particularly for developing countries.
Prediction of storey drift for reinforced concrete structures subjected to pulse-like ground motions using machine learning classification models
Authors: Wani Faisal Mehraj , Vemuri Jayaprakash , Chenna Rajaram 
Published: August 2023

PurposeNear-fault pulse-like ground motions have distinct and very severe effects on reinforced concrete (RC) structures. However, there is a paucity of recorded data from Near-Fault Ground Motions (NFGMs), and thus forecasting the dynamic seismic response of structures, using conventional techniques, under such intense ground motions has remained a challenge.Design/methodology/approachThe present study utilizes a 2D finite element model of an RC structure subjected to near-fault pulse-like ground motions with a focus on the storey drift ratio (SDR) as the key demand parameter. Five machine learning classifiers (MLCs), namely decision tree, k-nearest neighbor, random forest, support vector machine and Naïve Bayes classifier , were evaluated to classify the damage states of the RC structure.FindingsThe results such as confusion matrix, accuracy and mean square error indicate that the Naïve Bayes classifier model outperforms other MLCs with 80.0% accuracy. Furthermore, three MLC models with accuracy greater than 75% were trained using a voting classifier to enhance the performance score of the models. Finally, a sensitivity analysis was performed to evaluate the model's resilience and dependability.Originality/valueThe objective of the current study is to predict the nonlinear storey drift demand for low-rise RC structures using machine learning techniques, instead of labor-intensive nonlinear dynamic analysis.
Investigating the conceptual link between appearance-related self-discrepancies and retail therapy shopping behavior through motivational routes
Authors: Lee Jonggeun , Sadachar Amrut 
Published: August 2023

PurposeThe purpose of this study was to propose and examine a conceptual model delineating how two different types of appearance-related self-discrepancies (i.e. the ideal appearance self-discrepancy vs. the ought appearance self-discrepancy) influence retail therapy shopping behavior through motivational route (i.e. approach motivation vs. avoidance motivation).Design/methodology/approachThis study utilized the online survey for data collection. Using a national sample of 532 US consumers who had retail therapy shopping experience, the conceptual model was tested through various statistical techniques, including confirmatory factor analysis, exploratory factor analysis and structural equation modeling.FindingsResults revealed that the ought appearance self-discrepancy positively influenced retail therapy shopping behavior through avoidance motivation and emotion-focused coping strategy, whereas the ideal appearance self-discrepancy did not influence retail therapy shopping behavior. The results also suggested that the effect of two different types of appearance-related self-discrepancies on motivations in retail therapy shopping context varied depending on the gender (i.e. male vs. female).Originality/valueResults suggest implications regarding potential target market strategies to retailers and provide a better understanding of retail therapy shoppers' characteristics and psychological mechanisms for consumer researchers.
ChlOR, a GMC family oxidoreductase that evolved independently from the actinomycete, confers resistance to amphenicol antibiotics
Authors: Qian Yingying , Cheng Minggen , Lai Lin , Zhou Jing , Zylstra Gerben J. , Huang Xing 
Published: August 2023

AbstractOveruse of the amphenicol antibiotics chloramphenicol (CHL) and thiamphenicol (TAP) poses a great threat to ecosystem safety and human health. The strain, Nocardioides sp. LMS‐CY, Nocardioides sp. QY071 and Nocardioides sp. L‐11A, classified as a gram‐positive actinomycete, harbours a complete CHL metabolic pathway. However, the metabolic genes (clusters) involved in the entire pathway in gram‐positive actinomycetes are still limited. Here, chlORLMS, chlORQY071 and chlORL‐11A completely from the actinomycete Nocardioides spp. were found to act on the C1‐OH of the CHL/TAP side chain, directly converting CHL/TAP to 4‐nitrobenzaldehyde (PNBD)/4‐methylsulfonyl benzaldehyde (PMBD) and transforming PNBD/PMBD into 4‐nitrobenzyl alcohol (PNBM)/4‐methylsulfonyl phenyl methanol (PMBM). Furthermore, oxidoreductases can transform PNBM into 4‐nitrobenzoate (PNBA). The oxidoreductases ChlORLMS, ChlORQY071 and ChlORL‐11A were all classified as cellobiose dehydrogenases from the glucose methanol choline (GMC) family. Based on the Swiss‐Prot database, ChlORQY071 exhibited a lower identity (27.12%–35.10% similarity) with the reported oxidoreductases. Enzymatic and molecular docking analyses showed that ChlORQY071 and ChlORL‐11A from the two similar genomes were remarkably more effective in metabolizing CHL than ChlORLMS. Overall, the detailed resistance mechanism of CHL/TAP by actinomycete strains isolated from soil and livestock manure will provide insights into the occurrence of CHL/TAP resistance genes in the environment, resistance risk and bioremediation of CHL/TAP‐contaminated environments.
Living a ‘Digital Life’ and ready to cope with crises? Highlighting young adults' conceptions of crisis and emergency preparedness
Authors: Eriksson Mats 
Published: August 2023

AbstractIncreasing numbers of people are spending more and more time in digital landscapes, with many still unknown consequences for crisis and emergency management. This exploratory, in‐depth, qualitative interview study (N = 14) explores conceptions about local and individual crisis and emergency preparedness among a small group of young adults representing the most digitally savvy generation in Sweden. The results show that the respondents exhibit a complex and ambivalent attitude to crisis and emergency preparedness issues. Considering their digital habits and skills, the respondents emphasise their own responsibility, social ties, and expectations of help from the authorities and the local community when describing how they intend to face and manage a crisis in practice. This exploratory study contributes preliminary and tentative theoretical knowledge to a highly limited body of work specifically addressing citizens' crisis and emergency preparedness in a digitalized world.
Retrospective observational study of changes in serum cytokines and adiponectin with continuous plasma exchange with dialysis therapy for severe COVID‐19
Authors: Suzuki Yuya , Nakae Hajime , Satoh Kasumi , Hirasawa Nobuhisa , Kameyama Komei , Irie Yasuhito , Kitamura Toshiharu , Nara Tasuku , Maeno Kyohei , Yoshida Kenji , Okuyama Manabu 
Published: August 2023

AbstractIntroductionCoronavirus disease 2019 (COVID‐19) is associated with a marked increase in the inflammatory cytokines, IL‐6 and IL‐18. Blood purification therapy aimed at controlling cytokines is one treatment option; however, evidence of its effectiveness is needed. Plasma exchange with dialysis (PED) is a blood purification method involving selective plasma exchange with dialysate flowing through the outer hollow fiber of the plasma separator. In this retrospective study, we investigated the efficacy of continuous PED (cPED) over 48 h in five patients with severe COVID‐19.MethodsWe assessed changes in IL‐6 and IL‐18, as well as adiponectin (APN).ResultsThere were no significant differences in changes in IL‐6 and IL‐18, but there was a marked improvement in cases with abnormally high IL‐6 and IL‐18 levels at baseline. APN, which inhibits inflammatory cytokines, was significantly elevated post‐cPED.ConclusionOur results suggest that cPED therapy is an effective treatment for COVID‐19.
Mapping stream and floodplain geomorphometry with the Floodplain and Channel Evaluation Tool
Authors: Hopkins Kristina G. , Ahmed Labeeb , Claggett Peter R. , Lamont Samuel , Metes Marina J. , Noe Gregory B. 
Published: August 2023

AbstractBroad‐scale mapping of stream channel and floodplain geomorphic metrics is critical to improve the understanding of geomorphic change, biogeochemical processes, riverine habitat quality, and opportunities for management intervention. The Floodplain and Channel Evaluation Tool (FACET) was developed to provide an open‐source tool for automated processing of digital elevation models (DEMs) to generate regional‐scale estimates of bank height, channel width, floodplain width, and a suite of other fluvial geomorphic dimensions that can be summarized at the stream reach‐ or catchment‐scale. FACET was tested on 3‐m DEMs covering the Delaware River watershed and 85% of the Chesapeake Bay watershed in the United States (U.S.) and on 1‐m DEMs for a subset of the study area. Accuracy was assessed from data collected at 67 field sites in the study area. FACET successfully measured geomorphometry for over 270,000 stream reaches (88% of streams attempted) in the study area. Factors that reduced the ability of FACET to accurately estimate geomorphic metrics included errors in DEM hydro‐conditioning, gradually sloping banks, incised stream channels, and the use of fixed input parameters to define buffer lengths. Even with these limitations, FACET was able to map regional patterns in stream and floodplain geomorphometry providing a robust dataset that can enhance modeling and management efforts throughout the mid‐Atlantic region, U.S.
The interplay of episodic power in enabling and coercive budgetary designs in universities: A case study
Authors: Seneviratne Chaturika , Hoque Zahirul 
Published: August 2023

AbstractThis paper explores how power exercised at the individual level within existing power relations in a university influences its enabling and coercive budgeting forms. A qualitative case study in a Sri Lankan university involved interviews and document analysis. Findings demonstrate the dominance of coercive controls over enabling controls in the university due to the power and influence of dominant individuals stemming from ongoing struggles among those pursuing diverse interests and strategic actions. We contribute to the literature by demonstrating how power is mobilised by actors and its impact on the variations of the enabling and coercive budgetary forms and practice.
Abdominal aortic branches of red‐rumped agouti (Dasyprocta leporina Linnaeus, 1758)
Authors: Tertulino Moisés Dantas , de Sousa Ana Caroline Freitas Caetano , Gurgel João Vitor de Oliveira , Lopes Igor Renno Guimarães , Diniz João Augusto Rodrigues Alves , de Paula Valéria Veras , de Paula Antunes João Marcelo Azevedo , de Oliveira Radan Elvis Matias , de Oliveira Moacir Franco 
Published: August 2023

AbstractUnderstanding the cardiovascular system is fundamental in diagnosing pathologies and interpreting exams, such as contrast radiographs. In this context, the present study describes the collateral abdominal aorta artery branches of red‐rumped agouti. Ten red‐rumped agoutis, six males and four females, were assessed. The vascular system was perfused with Neoprene 450 latex coloured with a yellow pigment, dissected and analysed. Three euthanized animals were perfused with a barium sulfate solution (1 g mL−1) associated with latex Neoprene 450 at a 1:3 ratio to obtain contrast‐enhanced radiographs. The abdominal aorta emitted the celiac artery, which in turn originated the left gastric, hepatic and splenic arteries. The second collateral branch comprised the cranial mesenteric artery, followed by the renal arteries, which emitted the adrenal arteries, with the caudal emergence of the gonadal arteries. The caudal mesenteric artery appeared in a caudal direction. The abdominal aorta divided after reaching the pelvic cavity entrance, originating the right and left common iliac arteries. Before its bifurcation, the abdominal aorta dorsocaudally emitted its last collateral branch, the median sacral artery. The collateral branches of the aorta, therefore, resemble previously described rodent patterns, with few variations.