bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunosuppressive Effects of Annona muricata L. Leaf Extract on
Cellular and Humoral Immune Responses in Male Wistar Rats
Authors: Jantan Ibrahim , Abdul Wahab Siti Mariam , Husain Khairana , Arshad Laiba , Haque Md. Areeful , Mohd Fauzi Norsyahida , Nafiah Mohd Azlan , Das Srijit 
Published: December 2022

Background:
Annona muricata L. (Annonaceae) (AM)'s remarkable anti-inflammatory
and anti-cancer activities make it a targeted plant to be explored for its immunomodulatory properties.
Traditional practitioners have employed various components of AM to cure a variety of ailments,
including cancer, diabetes, and inflammation.

Objective:
The present study evaluated the immunosuppressive effects of 80% ethanol extract of of
AM leaves in male Wistar rats on different parameters of humoral and cellular immune responses.

Methods:
AM leaf extract (AMLE) was analyzed using UHPLC-MS/MS to profile its secondary
metabolites. AMLE was rich in polyphenols which include (epi)catechin-(epi)catechin-(epi) catechin,
caffeic acid, coumaroylquinic acid, hyperin, kaempferol, quinic acid and rutin. The rats were
administered 100, 200 and 400 mg/kg bw of the extract daily for 14 days. The effects of AMLE on
innate immune responses were determined by evaluating phagocytosis, neutrophils migration, reactive
oxygen species (ROS) release, CD11b/CD18 integrin expression, and ceruloplasmin, lysozyme
and myeloperoxidase (MPO) levels. The adaptive immune parameters were evaluated by immunizing
the rats with sheep red blood cells (sRBC) on day 0 and administered orally with AMLE for 14
days.

Results:
AMLE established significant immunosuppressive effects on the innate immune parameters
by inhibiting the neutrophil migration, ROS production, phagocytic activity and expression of
CD11b/CD18 integrin in a dose-dependent pattern. AMLE also suppressed ceruloplasmin, MPO
and lysozyme expressions in the rat plasma dose-dependently. AMLE dose-dependently inhibited T
and B lymphocytes proliferation, Th1 and Th2 cytokine production, CD4+ and CD8+ co-expression
in splenocytes, immunoglobulins (IgM and IgG) expression and the sRBC-induced swelling rate of
rat paw in delayed-type hypersensitivity (DTH).

Conclusion:
The strong inhibitory effects on the different parameters of humoral and cellular
responses indicate that AMLE has potential to be an important source of effective immunosuppressive
agents.
Chloroquinolone Carboxamide Derivatives as New Anti-HSV-1 Promising
Drugs
Authors: Cirne-Santos Claudio Cesar , Souza Mariana , de Melo Camilly Pestana Pires , Faro Letícia Villafranca , Fragel-Madeira Luciane , Giongo Viveca , Abreu Paula Alvarez , da Costa Santos Boechat Fernanda , de Oliveira Silva David , de Carvalho Tolentino Nathalia Motta , de Souza Barros Caroline , Castro Helena Carla , de Souza Marcos Costa , de Souza Maria Cecília Bastos Vieira , de Palmer Paixão Izabel Christina Nunes 
Published: December 2022

Background:
Since the emergence of HSV resistant strains, new antiviral agents have emerged and still are urgently needed, especially those with alternative targets.

Objective:
In this work, we evaluated new quinolone derivatives as anti-HSV.

Methods:
For this study, cells were infected and treated with different components to evaluate the profile of HSV replication in vitro. In addition, studies were performed to determine the pharma-cokinetic toxicity and profile of the compound.

Results:
Indeed the EC50 values of these promising molecules ranged between 8 μM and 32 μM. We have also showed that all compounds inhibited the expression of ICP27 viral proteins, which gives new insights in the search for new target for antiherpetic therapy. Chlorine in positions C6 and phosphonate in position C1 have shown to be important for viral inhibition. The chloroquinolone carboxamide derivatives fulfilled “Lipinsky Rule of Five” for good oral bioavailability and showed higher intestinal absorption and blood brain barrier penetration, as well as lower toxicity profile.

Conclusion:
Although the inhibition activities of chloroquinolone carboxamide derivatives were lower than acyclovir, they showed different modes of action in comparison to the drugs currently available. These findings encourage us to continue pre-clinical studies for the development of new anti-HSV-1 agents.
Non-vesicular Lipid Transport Machinery in Trichomonas vaginalis: Novel Drug Targets against Trichomoniasis
Authors: Das Koushik , Debnath Utsab , Rahaman Seikh Batin , Upadhyay Shashi , Das Dikhita 
Published: December 2022

Abstract:
Eukaryotic cells have separate membrane-enclosed organelles with distinct biochemical identities and specialized functions. The unique characteristics of each organelle are regulated by asymmetric distribution and intra-cellular trafficking of two important biomolecules, proteins and lipids. Non-vesicular lipid transport facilitated by lipid transfer proteins performs essential roles in intracellular lipid trafficking and homeostasis, while vesicular transport regulates protein traffick-ing. Comparative analysis of lipid transport machinery in protists could help us to understand the basis of parasitism and insight into eukaryotic evolution. Trichomonas vaginalis, a parasitic protist, greatly depends on receptor-ligand-mediated signaling pathways for cellular differentiation, nutrient uptake, secretion of virulence factors, and pathogenesis. Lipids, despite being key molecules of sig-naling cascades, have mechanisms of intracellular transport that are largely unexplored in T. vaginalis. We have identified a repertoire of seventeen potential lipid transfer protein (LTP) homo-logs in T. vaginalis based on a domain-based search on TrichDB (genome database of Trichomo-nas) coupled with bioinformatics analyses, which indicates the presence of well-organized lipid transport machinery in this parasite. We emphasized here their evolutionary uniqueness and conser-vation and discussed their potential implications for parasite biology in regard to future therapeutic targets against Trichomoniasis.
Dehydrogenation of Cyclohexanones to Phenols: A Mini Review
Authors: Du Yueying , Ren Dezhang , Huang Chenxin , Li Yang , Zhang Nahui , Huo Zhibao 
Published: December 2022

Background:
Phenol and its derivatives are important intermediates in the chemical industry,
especially the pharmaceutical and electronic industries. The synthesis of phenols has attracted
the attention of scientists due to their importance. Dehydrogenation of cyclohexanones is one of the
promising aromatization strategies for phenols manufacture because the raw materials are low cost
and stable. In recent years, some efficient and green methods with the use of H2, O2 and air, alkene,
H2 and O2-free are described.

Objective:
This mini-review will summarize some recent developments relating to the dehydrogenation
of cyclohexanones to phenols, along with their interesting mechanism aspects. The challenges
and future trends of the transformation will be prospected.

Conclusion:
The synthesis of phenols from the dehydrogenation of cyclohexanones has recently attracted
much attention. Some synthetic methods have been established, and interesting mechanisms
have been proposed in some cases. Lots of catalysts were developed for the transformation to afford
the corresponding product. Although the present methods still have drawbacks and limitations, it is
supposed that many novel methods would probably be developed in the near future.
Mitochondria in Early Life
Authors: He Ling , Maheshwari Akhil 
Published: December 2022

Abstract:
Mitochondria are highly-dynamic, membrane-bound organelles that generate most of the chemical energy needed to power the biochemical reactions in eukaryotic cells. These orga-nelles also communicate with the nucleus and other cellular structures to help maintain somatic homeostasis, allow cellular adaptation to stress, and help maintain the developmental trajectory. Mitochondria also perform numerous other functions to support metabolic, energetic, and epigenet-ic regulation in our cells. There is increasing information on various disorders caused by defects in intrinsic mitochondrial or supporting nuclear genes, on different organ systems. In this review, we have summarized the ultrastructural morphology, structural components, our current understanding of the evolution, biogenesis, dynamics, function, clinical manifestations of mitochondrial dysfunc-tion, and future possibilities. The implications of deficits in mitochondrial dynamics and signaling for embryo viability and offspring health are also explored. We present information from our own clinical and laboratory research in conjunction with information collected from an extensive search in the databases PubMed, EMBASE, and Scopus.
Health-related Quality of Life among Type 2 Diabetes Patients in Southern
Province of Saudi Arabia using WHOQOL-BREF: A Cross-section Study
Authors: Homady Areej , Albasheer Osama , Bajawi Amenah , Hamdi Shatha , Awaf Aisha , Madkhali Tahani , Sabai Abdallah , Zaino Mohammad R. , Somaili Mohammed 
Published: December 2022

Background:
Quality of life has become a more important health outcome with advancements
in medical therapies and disease management, leading to better lives for people in
general, particularly those living with chronic diseases. Diabetes has a direct impact on the physical,
psychological, and social aspects of personal health. This study aimed to determine healthrelated
quality of life (HRQOL) and its predictors in patients with type 2 diabetes.

Methods:
A cross-sectional study was carried out on 420 patients with type 2 diabetes who presented
to primary healthcare centers in Samtah Town, Saudi Arabia, from March 2017 to February
2018. The HRQOL of the study participants was determined using the World Health Organization
Quality of Life-BREF. Multi-level linear regression was employed to ascertain the factors associated
with HRQOL among patients with type 2 diabetes.

Results:
Female sex was significantly associated with higher HbA1c, anxiety, depression, and
stress scores and a lower HRQL in the physical, psychological, and environmental health subscales
than males (P<0.001). All subscales of HRQOL were significantly lower in the > 50 years age
group. When compared to those with low levels of education (illiterate, primary, or elementary
school), patients with secondary and bachelor’s degrees had significantly higher HRQOL in all
subscales (P<0.001). In addition, there were significant differences in glycemic control and HRQL
with a longer duration of diabetes, the presence of one or more diabetes complications, and the
presence of comorbid hypertension (P<0.001). However, no significant differences in the Depression
Anxiety and Stress Scale (DASS) index were observed with a longer duration of diabetes and
the presence of comorbid hypertension. Patients with diabetes on combined therapy and healthy
diet and exercise showed significantly higher HRQOL on physical functioning (P<0.001).

Conclusion:
Type 2 diabetes was significantly associated with impaired health-related quality of
life and mental health among study participants. Females appeared to have worse quality of life
and mental health than males. Age, duration of diabetes, comorbid hypertension, diabetes complications,
and level of education were significantly associated with lower HRQOL and mental health
scores. Healthy diet and exercise, when combined with hypoglycemic and insulin therapy, were
found to improve HRQOL and mental health among patients with diabetes. Scales based on a
broad definition of health, such as the WHOQOL-BREF, are appropriate for use in primary care
settings and can enhance patient management and care.
Salvia aucheri Exhibits Antihypertensive Activity in Hypertensive Rats
Authors: Azzane Amine , Amssayef Ayoub , Eddouks Mohamed 
Published: December 2022

Aims:
The present work aimed to assess the antihypertensive activity of Salvia aucheri.

Background:
Salvia aucheri (S. aucheri) is an aromatic and medicinal herb belonging to the Lamiaceae family. In Morocco, this plant is locally used for used to treat stomach, digestive disorders, rheumatism, and hypertension. Nevertheless, the effect of Salvia aucheri on hypertension has not yet been studied.

Objective:
The objective of this investigation was to evaluate the beneficial effect of the aqueous extract of S. aucheri leaves on arterial blood pressure, systolic blood pressure (SBP), mean blood pressure (MBP), diastolic blood pressure (DBP), and heart rate (HR) in normotensive and hypertensive rats. In addition, the effect of the aqueous extract of S. aucheri leaves on vasodilatation was assessed in isolated rat aortic rings with functional endothelium precontracted with epinephrine EP or KCl.

Methods::
The aqueous extract of the aerial parts of S. aucheri (AESA) was obtained, and its antihypertensive
ability was pharmacologically investigated in L-NAME hypertensive and normotensive
rats. The rats received AESA orally at two selected doses of 100 and 140 mg/kg for six hours
(acute experiment) and seven days (sub-chronic). Thereafter, systolic, diastolic, mean arterial
blood pressure and heart rate were evaluated. Moreover, the vasorelaxant activity of AESA was
performed in thoracic aortic ring rats. In addition, the mechanisms of action involved in the vasorelaxant
effect were studied.

Results:
The results indicated that AESA significantly reduced the systolic, diastolic, and mean arterial blood pressure in hypertensive rats over both single and repeated oral administration. However, AESA did not change the blood pressure parameters in normotensive rats. Concerning the results of vasorelaxant activity, the results showed that AESA was able to provoke potent vasorelaxant ability, which seems to be mediated through direct nitric oxide (NO) and NO-cyclic guanosine monophosphate pathways.

Conclusion:
The study elucidates the beneficial action of AESA as an antihypertensive and vasorelaxant agent.
Parents’ Experience and Healthcare Needs of having a Hospitalized Child with Burn Injury in Jordanian Hospitals: A Phenomenological Study Protocol
Authors: Al-Naimat Ibtihal Awwad , Abdel Razeq Nadin M. 
Published: December 2022

Background:A burn injury is a traumatic experience for the affected children and their parents. During the children’s burn injury treatment and hospitalization journey, parents experience many unmet needs. However, parents’ experiences and healthcare requirements during their children’s hospitalization have not been adequately addressed in the literature, especially in Jordan.Objective:A study is designed to gain in-depth knowledge of the parents’ experiences of children who require hospitalization for treatment of unintentional burn injuries.Methods:A descriptive phenomenological approach will be applied. Purposive sampling will be used to select parents from one teaching hospital and one public hospital. The researcher intends to collect data from the parents throughout their hospital experience from their child’s admission until after the child is discharged. The investigation and data gathering will continue until data saturation is reached. Data will be collected in three ways: multiple face-to-face semi-structured interviews with parents, the researcher’s field notes, and the parents’ diaries. The description of the phenomena will be performed following Colaizzi’s analysis method.Result/Discussion:The findings of this study will be an original contribution to the current literature in enhancing the understanding of pediatric burn victims’ parents’ experiences and healthcare needs during their child’s hospitalization. It is believed that the findings will help improve the quality of healthcare services provided to the parents of pediatric burn victims.Conclusion:This study protocol is shared to promote research quality and transparency and to inspire interested researchers.
Benzeneseleninic Acid Promoting the Selenofunctionalization of 2-
Naphthol Derivatives: Synthesis and Antioxidant Activity of 1-
Organoselanyl-naphthalen-2-ols
Authors: Mailahn Daniela H. , Araujo Daniela R. , Nobre Patrick C. , Fonseca Caren A. R. , Penteado Filipe , Lenardão Eder J. , Luchese Cristiane , Wilhelm Ethel A. , Perin Gelson 
Published: December 2022

Background:
We report the synthesis and antioxidant evaluation of 1-organoselanyl-naphthalen-2-ols. The title compounds were selectively prepared through the selenofunctionalization of 2-naphthol derivatives using benzeneseleninic acids as a selenium source.

Objective:
We aimed at synthesizing 1-organoselanyl-naphthalen-2-ol by functionalizing 2-naphthol with arylseleninic acids using glycerol as the solvent and further evaluating the pharmacological activity.

Methods:
The products were synthesized at 70 °C using glycerol as a solvent in an oil bath. Reactive species (RS) tests, DPPH radical scavenging activity assay, and ABTS+ radical scavenging activity assay were performed. A statistical analysis of the data was performed.

Results and Discussion:
A total of fifteen 1-organoselanyl-naphthalen-2-ols were selectively obtained in yields of 58-95% in 0.25-48 h of reaction. Additionally, all the synthesized seleno-derivatives exhibited antioxidant activity, as revealed by their ability to scavenge DPPH and ABTS+ radicals and reduce the reactive species (RS) levels.

Conclusion:
The synthesis of 1-arylselanyl-naphthalen-2-ols was developed under mild reaction conditions using benzeneseleninic acid derivatives in reactions with 2-naphthol. All synthesized 2-naphthol derivatives exhibited antioxidant activity, as revealed by their DPPH and ABTS+ radical scavenging activity and reduced RS levels.
Digitalization in early childhood education in Vietnam
Authors: Anh Truong Ngoc , Ha Nguyen Thi 
Published: December 2022

Digital transformation is the process of transforming interactions between actors on a traditional platform to interactions on a digital platform. This is the revolution that takes digitization or data as the foundation for development. This process is continuous and never stops. Digital transformation is a systematic process, so when all political, economic and social fields undergo digital transformation such as e-government, digital economy, digital businesses, digital banking, population, digital health, digital tourism... education is also in this inevitable trend. The article points out that digital transformation of education and training is a core part of taking advantage of the impact of the global industrial revolution 4.0. and how technology is transforming early childhood education. Why is technology important in early childhood education?
Entrevista com Eloy Rodrigues: “Não Haverá Ciência Aberta, Se Não For Abandonado o Uso Excessivo e Errado das Métricas”
Authors: Costa e Silva Elsa 
Published: December 2022

Eloy Rodrigues é membro do Grupo de Especialistas em Ciência 2.0/Ciência Aberta da Associação das Universidades Europeias, em representação do Conselho de Reitores das Universidades Portuguesas. Profundo conhecedor das questões da ciência aberta e um dos principais atores deste movimento em Portugal, é diretor dos Serviços de Documentação e Bibliotecas da Universidade do Minho (UMinho). É um dos principais advogados da adesão a práticas de ciência aberta e da inclusão do acesso aberto nas políticas institucionais. Coordenou a participação da UMinho em mais de uma dezena de projetos (como o OpenAIRE, https://www.openaire.eu/, e o FOSTER, https://www.fosteropenscience.eu/) financiados pela União Europeia e relacionados com os repositórios e a ciência aberta, sendo uma figura ímpar na concretização do acesso aberto a partir dos repositórios institucionais. Foi presidente do Conselho Executivo da Confederação de Repositórios de Acesso Aberto (https://www.coar-repositories.org/), de 2015 a 2021, e coordena, desde 2008, a equipa da UMinho que desenvolve o projeto Repositórios Científicos de Acesso Aberto de Portugal (www.rcaap.pt).
Comparative Performance Evaluation of the Public Health Surveillance Systems in 6 Gulf Cooperation Countries: Cross-sectional Study
Authors: Albali Nawaf , Almudarra Sami , Al-Farsi Yahya , Alarifi Abdullah , Al Wahaibi Adil , Penttinen Pasi 
Published: December 2022

Background
Evaluating public health surveillance systems is important to ensure that events of public health importance are appropriately monitored. Evaluation studies based on the Centers for Disease Control and Prevention (CDC) guidelines have been used to appraise surveillance systems globally. Previous evaluation studies undertaken in member countries of the Gulf Cooperation Council (GCC) were limited to specific illnesses within a single nation.

Objective
We aimed to evaluate public health surveillance systems in GCC countries using CDC guidelines and recommend necessary improvements to enhance these systems.

Methods
The CDC guidelines were used for evaluating the surveillance systems in GCC countries. A total of 6 representatives from GCC countries were asked to rate 43 indicators across the systems’ level of usefulness, simplicity, flexibility, acceptability, sensitivity, predictive value positive, representativeness, data quality, stability, and timeliness. Descriptive data analysis and univariate linear regression analysis were performed.

Results
All surveillance systems in the GCC covered communicable diseases, and approximately two-thirds (4/6, 67%, 95% CI 29.9%-90.3%) of them covered health care–associated infections. The mean global score was 147 (SD 13.27). The United Arab Emirates scored the highest in the global score with a rating of 167 (83.5%, 95% CI 77.7%-88.0%), and Oman obtained the highest scores for usefulness, simplicity, and flexibility. Strong correlations were observed between the global score and the level of usefulness, flexibility, acceptability, representativeness, and timeliness, and a negative correlation was observed between stability and timeliness score. Disease coverage was the most substantial predictor of the GCC surveillance global score.

Conclusions
GCC surveillance systems are performing optimally and have shown beneficial outcomes. GCC countries must use the lessons learned from the success of the systems of the United Arab Emirates and Oman. To maintain GCC surveillance systems so that they are viable and adaptable to future potential health risks, measures including centralized information exchange, deployment of emerging technologies, and system architecture reform are necessary.