bluuebunny/crossref_metadata_2025_split huggingface dataset:
N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction
Authors: Qi Zhangyang , Zhang Chi , Jian Huan , Hou Mengfan , Lou Yongfu , Kang Yi , Wang Wei , Lv Yigang , Shang Shenghui , Wang Chaoyu , Li Xueying , Feng Shiqing , Zhou Hengxing 
Published: May 2023

AbstractN1-Methyladenosine (m1A) is an abundant modification of transcripts, plays important roles in regulating mRNA structure and translation efficiency, and is dynamically regulated under stress. However, the characteristics and functions of mRNA m1A modification in primary neurons and oxygen glucose deprivation/reoxygenation (OGD/R) induced remain unclear. We first constructed a mouse cortical neuron OGD/R model and then used methylated RNA immunoprecipitation (MeRIP) and sequencing technology to demonstrate that m1A modification is abundant in neuron mRNAs and dynamically regulated during OGD/R induction. Our study suggests that Trmt10c, Alkbh3, and Ythdf3 may be m1A-regulating enzymes in neurons during OGD/R induction. The level and pattern of m1A modification change significantly during OGD/R induction, and differential methylation is closely associated with the nervous system. Our findings show that m1A peaks in cortical neurons aggregate at both the 5’ and 3’ untranslated regions. m1A modification can regulate gene expression, and peaks in different regions have different effects on gene expression. By analysing m1A-seq and RNA-seq data, we show a positive correlation between differentially methylated m1A peaks and gene expression. The correlation was verified by using qRT-PCR and MeRIP-RT-PCR. Moreover, we selected human tissue samples from Parkinson’s disease (PD) and Alzheimer’s disease (AD) patients from the Gene Expression Comprehensive (GEO) database to analyse the selected differentially expressed genes (DEGs) and differential methylation modification regulatory enzymes, respectively, and found similar differential expression results. We highlight the potential relationship between m1A modification and neuronal apoptosis following OGD/R induction. Furthermore, by mapping mouse cortical neurons and OGD/R-induced modification characteristics, we reveal the important role of m1A modification in OGD/R and gene expression regulation, providing new ideas for research on neurological damage.
The real-world efficacy and safety of faricimab in neovascular age-related macular degeneration: the TRUCKEE study – 6 month results
Authors: Khanani Arshad M. , Aziz Aamir A. , Khan Hannah , Gupta Ashwin , Mojumder Ohidul , Saulebayeva Aigerim , Abbey Ashkan M. , Almeida David R. P. , Avery Robert L. , Banda Himanshu K. , Barakat Mark R. , Bhandari Ramanath , Chang Emmanuel Y. , Haug Sara J. , London Nikolas J. S. , Mein Luke , Sheth Veeral S. , Wolfe Jeremy D. , Singer Michael A. , Danzig Carl J. 
Published: May 2023

Abstract
Background/Objective
Investigate real-world patients receiving faricimab for the treatment of neovascular age-related macular degeneration (nAMD).

Subjects/Methods
Multicenter, retrospective chart review was conducted on patients treated with faricimab for nAMD from February 2022 to September 2022. Collected data includes background demographics, treatment history, best-corrected visual acuity (BCVA), anatomic changes, and adverse events as safety markers. The main outcome measures are changes in BCVA, changes in central subfield thickness (CST) and adverse events. Secondary outcome measures included treatment intervals and presence of retinal fluid.

Results
After one injection of faricimab, all eyes (n = 376), previously-treated (n = 337) and treatment-naïve (n = 39) eyes demonstrated a + 1.1 letter (p = 0.035), a + 0.7 letter (p = 0.196) and a + 4.9 letter (p = 0.076) improvement in BCVA, respectively, and a − 31.3 μM (p < 0.001), a − 25.3 μM (p < 0.001) and a − 84.5 μM (p < 0.001) reduction in CST, respectively. After three injections of faricimab, all eyes (n = 94), previously-treated (n = 81) and treatment-naïve (n = 13) eyes demonstrated a + 3.4 letter (p = 0.03), a + 2.7 letter (p = 0.045) and a + 8.1 letter (p = 0.437) improvement in BCVA, and a − 43.4 μM (p < 0.001), a − 38.1 μM (p < 0.001) and a − 80.1 μM (p < 0.204) reduction in CST, respectively. One case of intraocular inflammation was observed after four injections of faricimab and resolved with topical steroids. One case of infectious endophthalmitis was treated with intravitreal antibiotics and resolved.

Conclusions
Faricimab has demonstrated improvement or maintenance of visual acuity for patients with nAMD, along with rapid improvement of anatomical parameters. It has been well-tolerated with low incidence of treatable intraocular inflammation. Future data will continue to investigate faricimab for real-world patients with nAMD.
RapaCaspase-9-based suicide gene applied to the safety of IL-1RAP CAR-T cells
Authors: Bouquet Lucie , Bôle-Richard Elodie , Warda Walid , Neto Da Rocha Mathieu , Trad Rim , Nicod Clémentine , Haderbache Rafik , Genin Delphine , Ferrand Christophe , Deschamps Marina 
Published: May 2023

AbstractEven if adoptive cell transfer (ACT) has already shown great clinical efficiency in different types of disease, such as cancer, some adverse events consistently occur, and suicide genes are an interesting system to manage these events. Our team developed a new medical drug candidate, a chimeric antigen receptor (CAR) targeting interleukin-1 receptor accessory protein (IL-1RAP), which needs to be evaluated in clinical trials with a clinically applicable suicide gene system. To prevent side effects and ensure the safety of our candidate, we devised two constructs carrying an inducible suicide gene, RapaCasp9-G or RapaCasp9-A, containing a single-nucleotide polymorphism (rs1052576) affecting the efficiency of endogenous caspase 9. These suicide genes are activated by rapamycin and based on the fusion of human caspase 9 with a modified human FK-binding protein, allowing conditional dimerization. RapaCasp9-G- and RapaCasp9-A-expressing gene-modified T cells (GMTCs) were produced from healthy donors (HDs) and acute myeloid leukemia (AML) donors. The RapaCasp9-G suicide gene demonstrated better efficiency, and we showed its in vitro functionality in different clinically relevant culture conditions. Moreover, as rapamycin is not pharmacologically inert, we also demonstrated its safe use as part of our therapy.
Risk factor analysis and nomogram establishment and verification of brain astrocytoma patients based on SEER database
Authors: Wang Ruiqi , Cui Jiaxue , Diao Yizhuo , Jin Chenxin , Chen Yongxing , Lv Xiupeng , Li Xiaofeng 
Published: May 2023

AbstractAstrocytoma is a common brain tumor that can occur in any part of the central nervous system. This tumor is extremely harmful to patients, and there are no clear studies on the risk factors for astrocytoma of the brain. This study was conducted based on the SEER database to determine the risk factors affecting the survival of patients with astrocytoma of the brain. Patients diagnosed with brain astrocytoma in the SEER database from 2004 to 2015 were screened by inclusion exclusion criteria. Final screened brain astrocytoma patients were classified into low grade and high grade according to WHO classification. The risk factors affecting the survival of patients with low-grade and high-grade brain astrocytoma were analyzed by univariate Kaplan–Meier curves and log-rank tests, individually. Secondly, the data were randomly divided into training set and validation set according to the ratio of 7:3, and the training set data were analyzed by univariate and multivariate Cox regression, and the risk factors affecting the survival of patients were screened and nomogram was established to predict the survival rates of patients at 3 years and 5 years. The area under the ROC curve (AUC value), C-index, and Calibration curve are used to evaluate the sensitivity and calibration of the model. Univariate Kaplan–Meier survival curve and log-rank test showed that the risk factors affecting the prognosis of patients with low-grade astrocytoma included Age, Primary site, Tumor histological type, Grade, Tumor size, Extension, Surgery, Radiation, Chemotherapy and Tumor number; risk factors affecting the prognosis of patients with high-grade astrocytoma include Age, Primary site, Tumor histological type, Tumor size, Extension, Laterality, Surgery, Radiation, Chemotherapy and Tumor number. Through Cox regression, independent risk factors of patients with two grades were screened separately, and nomograms of risk factors for low-grade and high-grade astrocytoma were successfully established to predict the survival rate of patients at 3 and 5 years. The AUC values of low-grade astrocytoma training set patients were 0.829 and 0.801, and the C-index was 0.818 (95% CI 0.779, 0.857). The AUC values of patients in the validation set were 0.902, 0.829, and the C-index was 0.774 (95% CI 0.758, 0.790), respectively. The AUC values of high-grade astrocytoma training set patients were 0.814 and 0.806, the C-index was 0.774 (95% CI 0.758, 0.790), the AUC values of patients in the validation set were 0.802 and 0.823, and the C-index was 0.766 (95% CI 0.752, 0.780), respectively, and the calibration curves of the two levels of training set and validation set were well fitted. This study used data from the SEER database to identify risk factors affecting the survival prognosis of patients with brain astrocytoma, which can provide some guidance for clinicians.
Evidence of inter-state coordination amongst state-backed information operations
Authors: Wang Xinyu , Li Jiayi , Srivatsavaya Eesha , Rajtmajer Sarah 
Published: May 2023

AbstractSince 2018, Twitter has steadily released into the public domain content discovered on the platform and believed to be associated with information operations originating from more than a dozen state-backed organizations. Leveraging this dataset, we explore inter-state coordination amongst state-backed information operations and find evidence of intentional, strategic interaction amongst thirteen different states, separate and distinct from within-state operations. We find that coordinated, inter-state information operations attract greater engagement than baseline information operations and appear to come online in service to specific aims. We explore these ideas in depth through two case studies on the coordination between Cuba and Venezuela, and between Russia and Iran.
Low eating self-efficacy is associated with unfavorable eating behavior tendencies among individuals with overweight and obesity
Authors: Oikarinen Noora , Jokelainen Terhi , Heikkilä Laura , Nurkkala Marjukka , Hukkanen Janne , Salonurmi Tuire , Savolainen Markku J. , Teeriniemi Anna-Maria 
Published: May 2023

AbstractSuccess in long-term weight management depends partly on psychological and behavioral aspects. Understanding the links between psychological factors and eating behavior tendencies is needed to develop more effective weight management methods. This population-based cross-sectional study examined whether eating self-efficacy (ESE) is associated with cognitive restraint (CR), uncontrolled eating (UE), emotional eating (EE), and binge eating (BE). The hypothesis was that individuals with low ESE have more unfavorable eating behavior tendencies than individuals with high ESE. Participants were classified as low ESE and high ESE by the Weight-Related Self-Efficacy questionnaire (WEL) median cut-off point. Eating behavior tendencies were assessed with Three Factor Eating Questionnaire R-18 and Binge Eating Scale, and additionally, by the number of difficulties in weight management. The difficulties were low CR, high UE, high EE, and moderate or severe BE. Five hundred and thirty-two volunteers with overweight and obesity were included in the study. Participants with low ESE had lower CR (p < 0.03) and higher UE, EE, and BE (p < 0.001) than participants with high ESE. Thirty-nine percent of men with low ESE had at least two difficulties in successful weight control while this percentage was only 8% in men with high ESE. In women, the corresponding figures were 56% and 10%. The risk of low ESE was increased by high UE [OR 5.37 (95% CI 1.99–14.51)], high EE [OR 6.05 (95% CI 2.07–17.66)], or moderate or severe BE [OR 12.31 (95% CI 1.52–99.84)] in men, and by low CR [OR 5.19 (95% CI 2.22–12.18)], high UE [OR 7.20 (95% CI 2.41–19.22)], or high EE [OR 23.66 (95% CI 4.79–116.77)] in women. Low ESE was associated with unfavorable eating behavior tendencies and multiple concomitant difficulties in successful weight loss promotion. These eating behavior tendencies should be considered when counseling patients with overweight and obesity.
Association of accelerometer-measured physical activity, back static muscular endurance and abdominal obesity with radicular pain and non-specific low back pain
Authors: Bayartai Munkh-Erdene , Määttä Juhani , Karppinen Jaro , Oura Petteri , Takatalo Jani , Auvinen Juha , Raija Korpelainen , Niemelä Maisa , Luomajoki Hannu 
Published: May 2023

AbstractLow back pain (LBP) is the leading cause of disability worldwide and often associated with lifestyle factors. However, studies further examining the role of these lifestyle factors in non-specific low back pain in comparison with radicular pain are sparse. The aim of this cross sectional study was to investigate how diverse lifestyle factors are associated with LBP. The study population of 3385 middle aged adults with and without low back pain was drawn from a large Birth 1966 Cohort. Outcome measures were steps per day, abdominal obesity, physical activity and endurance of the back muscles. Back static muscular endurance, abdominal obesity and physical activity were measured by means of the Biering–Sørensen test, waist circumference and a wrist worn accelerometer, respectively. Logistic regression analysis was applied to estimate associations of back static muscular endurance, abdominal obesity and accelerometer-measured physical activity with non-specific low back pain and radicular pain. An additional 1000 steps per day were associated with 4% lower odds of having non-specific low back pain. Participants with abdominal obesity had 46% higher odds of having radicular pain, whereas increases of 10 s in back static muscular endurance and 10 min in daily vigorous physical activity were associated with 5% and 7% lower odds of having radicular pain, respectively. In this population-based study, non-specific low back pain and radicular pain were associated with different lifestyle and physical factors at midlife. Non-specific low back pain was associated only with the average daily number of steps, whereas abdominal obesity was the strongest determinant of radicular pain, followed by vigorous physical activity and back static muscular endurance. The findings of this study contribute to better understand the role of lifestyle factors in both non-specific low back pain and radicular pain. Future longitudinal studies are required to explore causality.
The complex regulation of competence in Staphylococcus aureus under microaerobic conditions
Authors: Feng Shi Yuan , Hauck Yolande , Morgene Fedy , Mohammedi Roza , Mirouze Nicolas 
Published: May 2023

AbstractTo perform natural transformation, one of the three main Horizontal Gene Transfer mechanisms, bacteria need to enter a physiological differentiated state called genetic competence. Interestingly, new bacteria displaying such aptitude are often discovered, and one of the latest is the human pathogen Staphylococcus aureus.Here, we show an optimized protocol, based on planktonic cells cultures, leading to a large percentage of the population activating the development of competence and a significant improvement of S. aureus natural transformation efficiencies. Taking advantage of these conditions, we perform transcriptomics analyses to characterize the regulon of each central competence regulator. SigH and ComK1 are both found essential for activating natural transformation genes but also important for activation or repression of peripheral functions. Even though ComK2 is not found important for the control of transformation genes, its regulon shows an important overlap with that of SigH and ComK1. Finally, we propose that microaerobic conditions, sensed by the SrrAB two-component system, are key to activate competence in S. aureus.
Three-dimensional kinematics of the India–Eurasia collision
Authors: Wang Lifeng , Barbot Sylvain 
Published: May 2023

AbstractThe collision between India and Eurasia mobilizes multiple processes of continental tectonics. However, how deformation develops within the lithosphere across the Tibetan Plateau is still poorly known and a synoptic view is missing. Here, we exploit an extensive geodetic observatory to resolve the kinematics of this diffuse plate boundary and the arrangement of various mechanisms down to upper-mantle depths. The three-dimensional velocity field is compatible with continental underthrusting below the central Himalayas and with delamination rollback below the western syntaxis. The rise of the Tibetan Plateau occurs by shortening in the Indian and Asian crusts at its southern and northwestern margins. The subsidence of Central Tibet is associated with lateral extrusion and attendant lithospheric thinning aided by the downwelling current from the opposite-facing Indian and Asian collisions. The current kinematics of the Indian-Eurasian collision may reflect the differential evolution of the inner and outer Tibetan Plateau during the late Cenozoic.
A review: Monitoring situational awareness of smart grid cyber‐physical systems and critical asset identification
Authors: Alrowaili Yazeed , Saxena Neetesh , Srivastava Anurag , Conti Mauro , Burnap Pete 
Published: May 2023

AbstractCyber‐Physical Systems (CPSs) are becoming more automated and aimed to be as efficient as possible by enabling integration between their operations and Information Technology (IT) resources. In combination with production automation, these systems need to identify their assets and the correlation between them; any potential threats or failures alert the relevant user/department and suggest the appropriate remediation plan. Moreover, identifying critical assets in these systems is essential. With numerous research and technologies available, assessing IT assets nowadays can be straightforward to implement. However, there is one significant issue of evaluating operational technology critical assets since they have different characteristics, and traditional solutions cannot work efficiently. This study presents the necessary background to attain the appropriate approach for monitoring critical assets in CPSs' Situational Awareness (SA). Additionally, the study presents a broad survey supported by an in‐depth review of previous works in three important aspects. First, it reviews the applicability of possible techniques, tools and solutions that can be used to collect detailed information from such systems. Secondly, it covers studies that were implemented to evaluate the criticality of assets in CPSs, demonstrates requirements for critical asset identification, explores different risks and failure techniques utilised in these systems and delves into approaches to evaluate such methods in energy systems. Finally, this paper highlights and analyses SA gaps based on existing solutions, provides future directions and discusses open research issues.
Quiet-time suprathermal ions in the inner heliosphere during the rising phase of solar cycle 25
Authors: Mason G. M. , Ho G. C. , Allen R. C. , Kouloumvakos A. , Wimmer-Schweingruber R. F. , Rodriguez-Pacheco J. 
Published: May 2023

Context. The Solar Orbiter spacecraft made its first close perihelion passes in 2022, reaching 0.32 au on 26 March and 0.29 au on 12 October. Transient activity was relatively low, making it possible to perform measurements of the quiet-time suprathermal ion pool over multi-day periods.
Aims. The inner heliosphere suprathermal ion pool is a source of seed particles accelerated by coronal mass ejection-driven shocks. Determining its constituents and their dependence on solar activity, location, and time is therefore critical to building physical models of particle acceleration from solar events.
Methods. By selecting low-activity periods on Solar Orbiter during perihelia passes, and comparing them with a nearly identical monitoring instrument at 1 au, the observed differences in intensities can be related to factors such as distance from the Sun, transient events, and interacting solar wind streams.
Results. Below ∼1 MeV/nucleon, the observed quiet-time spectra at 0.32 au for H, 4He, 3He, and Fe rise toward low energies, as observed previously during longer periods at 1 au, and they show a heavy ion composition that has markers of impulsive solar flare material, such as relatively high 3He:4He, and a high Fe/O ratio. The proton and helium abundances are much higher, consistent with a source in corotating interaction regions. Surveying all semi-quiet times during the mission, there is only a modest (∼15%) increase in the fluences in the inner heliosphere compared to 1 au, indicating small gradients in these populations between 1 and 0.3 au.
Complications des chirurgies urogénitales du mâle
Authors: Lallemand Élodie Anne 
Published: May 2023

Les chirurgies urogénitales du cheval mâle, hormis la castration, concernent principalement les chirurgies du pénis, de l’urètre et de la vessie. Les complications les plus fréquentes communes aux chirurgies de ces 3 structures sont notamment les saignements et hémorragies, les œdèmes et la déhiscence de plaie, comme de nombreuses autres chirurgies. Les complications plus spécifiques sont les irritations cutanées causées par l’urine pour les chirurgies du pénis et de l’urètre ; les obstructions urinaires, notamment s’il y a développement de sténoses urétrales qui peuvent être favorisées par l’aspect spiculé des calculs urinaires ; et les récidives tumorales lors de traitement de tumeurs. La meilleure stratégie reste la prévention, en particulier en soignant la réalisation des procédures chirurgicales. Une hémorragie sévère postopératoire pourra nécessiter une reprise chirurgicale, de même que les sténoses urétrales.
Imaging Recommendations for Positron Emission Tomography (PET) in Oncology
Authors: Chaturvedi Arvind K. , Mahajan Abhishek , Sen Saugata , Swamy Shivakumar S. , Shah Diva , Chakrabarty Nivedita 
Published: May 2023

AbstractCancer is one of the leading causes of morbidity and mortality. Imaging studies are central to the initial staging and follow-up management of cancers. In the past, oncologists have largely relied on anatomical imaging for staging, restaging, and therapy monitoring. The introduction of positron emission tomography-computed tomography (PET-CT) and its availability has transformed the practice of cancer imaging. PET-CT is an imaging technique that provides complementary information to imaging by CT or magnetic resonance imaging alone as it incorporates functional imaging to the anatomic information. It actually embeds tumor biology on the anatomical image. There are significant contributions of the CT component in adding value to the strength of PET-CT. PET-CT is useful for initial staging of cancers. It is particularly useful in detection of distant metastases, in assessing response to therapy and in detection of recurrence. Its utility in restaging and follow-up of cancers is now well established. Its role varies across different primary cancer sites. To cover the role of PET-CT in all cancer types is neither the intention nor feasible in a single article. In this article, an attempt will be made to highlight the generic concepts of PET-CT imaging and its role in primary staging and post-therapy follow-up across some common malignancies. Its pitfalls and limitations will also be discussed.