bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping of structural arrangement of cells and collective calcium transients: an integrated framework combining live cell imaging using confocal microscopy and UMAP-assisted HDBSCAN-based approach
Authors: Gare Suman , Chel Soumita , Abhinav T K , Dhyani Vaibhav , Jana Soumya , Giri Lopamudra 
Published: January 2023

AbstractLive cell calcium (Ca2+) imaging is one of the important tools to record cellular activity during in vitro and in vivo preclinical studies. Specially, high-resolution microscopy can provide valuable dynamic information at the single cell level. One of the major challenges in the implementation of such imaging schemes is to extract quantitative information in the presence of significant heterogeneity in Ca2+ responses attained due to variation in structural arrangement and drug distribution. To fill this gap, we propose time-lapse imaging using spinning disk confocal microscopy and machine learning-enabled framework for automated grouping of Ca2+ spiking patterns. Time series analysis is performed to correlate the drug induced cellular responses to self-assembly pattern present in multicellular systems. The framework is designed to reduce the large-scale dynamic responses using uniform manifold approximation and projection (UMAP). In particular, we propose the suitability of hierarchical DBSCAN (HDBSCAN) in view of reduced number of hyperparameters. We find UMAP-assisted HDBSCAN outperforms existing approaches in terms of clustering accuracy in segregation of Ca2+ spiking patterns. One of the novelties includes the application of non-linear dimension reduction in segregation of the Ca2+ transients with statistical similarity. The proposed pipeline for automation was also proved to be a reproducible and fast method with minimal user input. The algorithm was used to quantify the effect of cellular arrangement and stimulus level on collective Ca2+ responses induced by GPCR targeting drug. The analysis revealed a significant increase in subpopulation containing sustained oscillation corresponding to higher packing density. In contrast to traditional measurement of rise time and decay ratio from Ca2+ transients, the proposed pipeline was used to classify the complex patterns with longer duration and cluster-wise model fitting. The two-step process has a potential implication in deciphering biophysical mechanisms underlying the Ca2+ oscillations in context of structural arrangement between cells.
Histology of Convergent Probing Appendages in Mormyridae
Authors: Peterson R D , Evans A J , Hernandez L P 
Published: January 2023

SynopsisMormyridae is an early diverging family of Teleostean fishes that produce an electric field for navigation and communication using an electric organ. This clade has a diverse array of soft-tissue rostral appendages, such as the chin-swelling, the Schnauzenorgan, and the tubesnout combined with a Schnauzenorgan, that have evolved multiple times. Here we assess if macroscopically convergent, soft-tissue rostral appendages are also histologically convergent. Further, we investigate how the histology of these appendages can inform their function. We sampled independent gains of the chin-swelling and Schnauzenorgan to understand similarities and differences in their anatomies. We show that macroscopically convergent rostral appendages are also convergent at a histological level, and different types of rostral appendages share a similar anatomy; that said, minor differences likely relate to their specific functions. Based on a comparison of the skeletal muscle distribution and the differing attachment shapes of each appendage to the dentary, we conclude that the Schnauzenorgan is capable of a wider range of movements than the chin swelling. Furthermore, the anatomy suggests that these soft-tissue rostral appendages likely function as electrosensory foveas (i.e., an appendage that focuses a sensory system). Lastly, these histological data support the hypothesis that the chin swelling may be a precursor to the Schnauzenorgan.
Jaw1/LRMP is associated with the maintenance of Golgi ribbon structure
Authors: Okumura Wataru , Tadahira Kazuko , Kozono Takuma , Tamura-Nakano Miwa , Sato Hiroyuki , Matsui Hitomi , Dohi Taeko , Rohrer Jack , Tonozuka Takashi , Nishikawa Atsushi 
Published: January 2023

Abstract
Jaw1/LRMP is a membrane protein that is localized to the endoplasmic reticulum and outer nuclear membrane. Previously, we revealed that Jaw1 functions to maintain nuclear shape by interacting with microtubules as a Klarsicht/ANC-1/Syne/homology (KASH) protein. The loss of several KASH proteins causes defects in the position and shape of the Golgi apparatus as well as the nucleus, but the effects of Jaw1 depletion on the Golgi apparatus were poorly understood. Here, we found that siRNA-mediated Jaw1 depletion causes Golgi fragmentation with disordered ribbon structure in the melanoma cell, accompanied by the change in the localization of the Golgi-derived microtubule network. Thus, we suggest that Jaw1 is a novel protein to maintain the Golgi ribbon structure, associated with the microtubule network.
How Verb Tense Shapes Persuasion
Authors: Packard Grant , Berger Jonah , Boghrati Reihane 
Published: January 2023

AbstractWhen sharing information and opinions about products, services, and experiences, communicators often use either past or present tense (e.g., “That restaurant was great” or “That restaurant is great”). Might such differences in verb tense shape communication’s impact, and if so, how? A multimethod investigation, including eight studies conducted in the field and lab, demonstrates that using present (vs. past) tense can increase persuasion. Natural language processing of over 500,000 online reviews in multiple product and service domains, for example, illustrates that reviews that use more present tense are seen as more helpful and useful. Follow-up experiments demonstrate that shifting from past to present tense increases persuasion and illustrate the underlying process through both mediation and moderation. When communicators use present (rather than past) tense to express their opinions and experiences, it suggests that they are more certain about what they are saying, which increases persuasion. These findings shed light on how language impacts consumer behavior, highlight how a subtle, yet central linguistic feature shapes communication, and have clear implications for persuasion across a range of situations.
Risk prediction models for endometrial cancer: development and validation in an international consortium
Authors: Shi Joy , Kraft Peter , Rosner Bernard A , Benavente Yolanda , Black Amanda , Brinton Louise A , Chen Chu , Clarke Megan A , Cook Linda S , Costas Laura , Dal Maso Luigino , Freudenheim Jo L , Frias-Gomez Jon , Friedenreich Christine M , Garcia-Closas Montserrat , Goodman Marc T , Johnson Lisa , La Vecchia Carlo , Levi Fabio , Lissowska Jolanta , Lu Lingeng , McCann Susan E , Moysich Kirsten B , Negri Eva , O'Connell Kelli , Parazzini Fabio , Petruzella Stacey , Polesel Jerry , Ponte Jeanette , Rebbeck Timothy R , Reynolds Peggy , Ricceri Fulvio , Risch Harvey A , Sacerdote Carlotta , Setiawan Veronica W , Shu Xiao-Ou , Spurdle Amanda B , Trabert Britton , Webb Penelope M , Wentzensen Nicolas , Wilkens Lynne R , Xu Wang Hong , Yang Hannah P , Yu Herbert , Du Mengmeng , De Vivo Immaculata 
Published: January 2023

Abstract

Background
Endometrial cancer risk stratification may help target interventions, screening, or prophylactic hysterectomy to mitigate the rising burden of this cancer. However, existing prediction models have been developed in select cohorts and have not considered genetic factors.

Methods
We developed endometrial cancer risk prediction models using data on postmenopausal White women aged 45-85 years from 19 case-control studies in the Epidemiology of Endometrial Cancer Consortium (E2C2). Relative risk estimates for predictors were combined with age-specific endometrial cancer incidence rates and estimates for the underlying risk factor distribution. We externally validated the models in 3 cohorts: Nurses’ Health Study (NHS), NHS II, and the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial.

Results
Area under the receiver operating characteristic curves for the epidemiologic model ranged from 0.64 (95% confidence interval [CI] = 0.62 to 0.67) to 0.69 (95% CI = 0.66 to 0.72). Improvements in discrimination from the addition of genetic factors were modest (no change in area under the receiver operating characteristic curves in NHS; PLCO = 0.64 to 0.66). The epidemiologic model was well calibrated in NHS II (overall expected-to-observed ratio [E/O] = 1.09, 95% CI = 0.98 to 1.22) and PLCO (overall E/O = 1.04, 95% CI = 0.95 to 1.13) but poorly calibrated in NHS (overall E/O = 0.55, 95% CI = 0.51 to 0.59).

Conclusions
Using data from the largest, most heterogeneous study population to date (to our knowledge), prediction models based on epidemiologic factors alone successfully identified women at high risk of endometrial cancer. Genetic factors offered limited improvements in discrimination. Further work is needed to refine this tool for clinical or public health practice and expand these models to multiethnic populations.
Bystander signals from low- and high-dose irradiated human primary fibroblasts and keratinocytes modulate the inflammatory response of peripheral blood mononuclear cells
Authors: Testard Isabelle , Garcia-Chartier Elizabeth , Issa Amani , Collin-Faure Véronique , Aude-Garcia Catherine , Candéias Serge M 
Published: January 2023

AbstractIrradiated cells can propagate signals to neighboring cells. Manifestations of these so-called bystander effects (BEs) are thought to be relatively more important after exposure to low- vs high-dose radiation and can be mediated via the release of secreted molecules, including inflammatory cytokines, from irradiated cells. Thus, BEs can potentially modify the inflammatory environment of irradiated cells. To determine whether these modifications could affect the functionality of bystander immune cells and their inflammatory response, we analyzed and compared the in vitro response of primary human fibroblasts and keratinocytes to low and high doses of radiation and assessed their ability to modulate the inflammatory activation of peripheral blood mononuclear cells (PBMCs). Only high-dose exposure resulted in either up- or down-regulation of selected inflammatory genes. In conditioned culture media transfer experiments, radiation-induced bystander signals elicited from irradiated fibroblasts and keratinocytes were found to modulate the transcription of inflammatory mediator genes in resting PBMCs, and after activation of PBMCs stimulated with lipopolysaccharide (LPS), a strong inflammatory agent. Radiation-induced BEs induced from skin cells can therefore act as a modifier of the inflammatory response of bystander immune cells and affect their functionality.
Exosome-like vesicles released from ob/ob mouse adipose tissue enhance cell survival of cells with radiation-induced genomic instability
Authors: Ariyoshi Kentaro , Fujishima Yohei , Goh Valerie Swee Ting , Nakata Akifumi , Kasai Kosuke , Yoshida Mitsuaki A , Miura Tomisato 
Published: January 2023

AbstractMultiple epidemiological studies have shown that obesity is a serious risk factor for cancer development. While the underlying mechanisms between obesity and cancer are still unknown, obesity disrupts the role of adipocytes in energy homeostasis, and the alteration of adipokine, insulin and sex steroid signaling. Recently, it has been identified that adipose tissue-derived exosome-like vesicles (ELVs) regulate metabolic homeostasis. In this study, we collected ELVs from adipose tissue of an obese mouse (ob/ob) strain and control mouse (C57BL/6) strain, and checked whether adipose ELVs influence radiation-induced cell death on mouse fibroblast cells (m5S). Furthermore, we analyzed the micronucleus (MN) frequency in survived cells after radiation exposure to investigate the effect of ELVs on radiation-induced genomic instability. We first observed that ELVs from control and obese mice showed enhanced colony forming ability in un-irradiated m5S cells. However, enhanced survival was observed only in 3 Gy-irradiated m5S cells with obese ELV treatment. Despite no ELV effect on colony size, interestingly, the frequency of MN in survived m5S cells after 3 Gy irradiation was elevated when treated obese ELVs compared to control ELVs. These results suggested that obese mouse adipose ELVs could enhance the survival of irradiated cells harboring increased radiation-induced genomic instability.
Comparison of nutritional value, bioactivity, and volatile compounds of soybean meal–corn bran mixed substrates fermented by different microorganisms
Authors: Huang Jinqing , Dai Yuming , Huang Tao , Du Tonghao , Xiong Tao 
Published: January 2023

Abstract
To evaluate the impact of fermentation with different microorganisms on the nutritional quality and bioactivity of soybean meal–corn bran mixed substrates (MS), five lactic acid bacteria (LAB) strains, two Bacillus, and two yeast strains with excellent probiotics were selected for solid-state fermentation of soybean meal and corn bran MS. The fermented mixed substrate (FMS) inoculated with Lacticaseibacillus casei, Lactobacillus fermentum, Lactiplantibacillus plantarum, and Lactobacillus acidophilus presents lower risk of infection with pathogenic bacteria, probably due to their low pH and high lactate content. Compared to the FMS with LAB and yeast, Bacillus subtilis and B. pumilus showed significant improvements in nutritional quality and bioactivity, including TCA-SP, small peptide, free amino acids, total phenol, and protein digestibility. More than 300 volatile compounds were identified in FMS, including alcohols, ketones, aldehydes, esters, acids, ethers, furans, pyrazines, benzene, phenols, amines, alkanes, and others. FMS with Bacillus was characterized as containing a greater number of compounds such as ketones, aldehydes, and pyrazines. This study showed that microbial fermented feeds differed with various microorganism, and fermentation was an effective way to improve the quality of soybean meal–corn bran mixed feeds. This study might be the basis for excellent strains screening for multi-microbial combined fermentation in the future.
Improving Star Cluster Age Estimates in PHANGS-HST Galaxies and the Impact on Cluster Demographics in NGC 628
Authors: Whitmore Bradley C , Chandar Rupali , Lee Janice C , Floyd Matthew , Deger Sinan , Lilly James , Minsley Rebecca , Thilker David A , Boquien Médéric , Dale Daniel A , Henny Kiana , Scheuermann Fabian , Barnes Ashley T , Bigiel Frank , Emsellem Eric , Glover Simon , Grasha Kathryn , Groves Brent , Hannon Stephen , Klessen Ralf S , Kreckel Kathryn , Kruijssen J M Diederik , Larson Kirsten L , Leroy Adam , Mok Angus , Pan Hsi-An , Pinna Francesca , Sánchez-Blázquez Patricia , Schinnerer Eva , Sormani Mattia C , Watkins Elizabeth , Williams Thomas 
Published: January 2023

ABSTRACT
A long-standing problem when deriving the physical properties of stellar populations is the degeneracy between age, reddening, and metallicity. When a single metallicity is used for all the star clusters in a galaxy, this degeneracy can result in ‘catastrophic’ errors for old globular clusters. Typically, approximately 10–20 per cent of all clusters detected in spiral galaxies can have ages that are incorrect by a factor of 10 or more. In this paper, we present a pilot study for four galaxies (NGC 628, NGC 1433, NGC 1365, and NGC 3351) from the PHANGS-HST survey. We describe methods to correct the age-dating for old globular clusters, by first identifying candidates using their colours, and then reassigning ages and reddening based on a lower metallicity solution. We find that young ‘Interlopers’ can be identified from their Hα flux. CO (2-1) intensity or the presence of dust can also be used, but our tests show that they do not work as well. Improvements in the success fraction are possible at the ≈15 per cent level (reducing the fraction of catastrophic age-estimates from between 13 and 21 per cent, to between 3  and 8 per cent). A large fraction of the incorrectly age-dated globular clusters are systematically given ages around 100 Myr, polluting the younger populations as well. Incorrectly age-dated globular clusters significantly impact the observed cluster age distribution in NGC 628, which affects the physical interpretation of cluster disruption in this galaxy. For NGC 1365, we also demonstrate how to fix a second major age-dating problem, where very dusty young clusters with E(B − V) &gt; 1.5 mag are assigned old, globular-cluster like ages. Finally, we note the discovery of a dense population of ≈300 Myr clusters around the central region of NGC 1365 and discuss how this results naturally from the dynamics in a barred galaxy.
Identifying meteorite droppers among the population of bright ‘sporadic’ bolides imaged by the Spanish Meteor Network during the spring of 2022
Authors: Peña-Asensio E , Trigo-Rodríguez J M , Rimola A , Corretgé-Gilart M , Koschny D 
Published: January 2023

ABSTRACTThe extraordinary weather conditions available between February and March 2022 over Spain have allowed us to analyse the brightest fireballs recorded by the monitoring stations of the Spanish Meteor Network (SPMN). We study the atmospheric flight of 15 large meteoroids to determine if they are meteorite dropper events to prepare campaigns to search for freshly fallen extraterrestrial material. We investigate their origins in the Solar system and their dynamic association with parent bodies and meteoroid streams. Employing our python pipeline 3d-firetoc, we reconstruct the atmospheric trajectory utilizing ground-based multistation observations and compute the heliocentric orbit. In addition, we apply an ablation model to estimate the initial and terminal mass of each event. Using a dissimilarity criterion and propagating backward in time, we check the connection of these meteoroids with known complexes and near-Earth objects. We also calculate if the orbits are compatible with recent meteoroid ejections. We find that ∼27 per cent of these fireballs are dynamically associated with minor meteoroid streams and exhibit physical properties of cometary bodies, as well as one associated with a near-Earth asteroid. We identify two meteorite-producing events; however, the on-site search was unsuccessful. By considering that these fireballs are mostly produced by cm-sized rocks that might be the fragmentation product of much larger meteoroids, our findings emphasize the idea that the population of near-Earth objects is a source of near-term impact hazards, existing large Earth-colliding meteoroids in the known complexes.
A ‘coronal-mass-ejection’ model for flares in Sagittarius A*
Authors: Lin Xi , Li Ya-Ping , Yuan Feng 
Published: January 2023

ABSTRACT
High-resolution near-infrared observations with GRAVITY instrument have revealed rapid orbital motions of a hotspot around Sagittarius A* (Sgr A*), the supermassive black hole in our Galactic Centre, during its three bright flares. The projected distances of the spot to the black hole are measured and seem to increase with time. The values of distance, combined with the measured orbiting time, imply that the spot is rotating with a super-Keplerian velocity. These results are hard to understand if the spot stays within the accretion flow thus provides strong constraints on theoretical models for flares. Previously we have proposed a ‘CME’ model for the flares by analogy with the coronal-mass-ejection model in solar physics. In that model, magnetic reconnection occurred at the surface of the accretion flow results in the formation of flux ropes, which are then ejected out. Energetic electrons accelerated in the current sheet flow into the flux rope region and their radiation is responsible for the flares. In this paper, we apply the model to the interpretation of the GRAVITY results by calculating the dynamics of the ejected flux rope, the evolution of the magnetic field and the energy distribution of accelerated electrons, and the radiation of the system. We find that the model can well explain the observed light curve of the flares, the time-dependent distance, and the super-Keplerian motion of the hotspot. It also explains why the light curve of some flares has double peaks.
Spectrophotometric analysis of magnetic white dwarf – II. Helium-rich compositions
Authors: Hardy François , Dufour Patrick , Jordan Stefan 
Published: January 2023

ABSTRACT
We present an analysis of all single white dwarf stars known to exhibit spectroscopic signatures of neutral helium line splitting due to the presence of a strong magnetic field. Using state-of-the-art models taking into account the effects of magnetic fields on the synthetic spectra, we determine effective temperatures, surface gravities, and masses for the stars in our sample. Our analysis uses data from the second and third Gaia (early) data releases, photometric data from diverse surveys such as the Sloan Digital Sky Survey and Pan-STARRS, and archived spectroscopic data. We are able to successfully reproduce the spectra of eight objects using an offset dipole geometry while several others seem to require either a more complex geometry or a different chemical composition. We also highlight a group of hot featureless white dwarfs that are most probably highly magnetic objects whose spectra are completely smeared due to the field strength distribution across the surface.
The study on transiting systems HAT-P-13, HAT-P-16, and WASP-32 through combining ground-based and TESS photometry
Authors: Sun L , Gu S , Wang X , Bai L , Schmitt J H M M , Perdelwitz V , Ioannidis P 
Published: January 2023

ABSTRACT
High-precision transit photometry supplies ideal opportunities for detecting new exoplanets and characterizing their physical properties, which usually encode valuable information for unveiling the planetary structure, atmosphere, and dynamical history. We present revised properties of three transiting systems (i.e. HAT-P-13, HAT-P-16, and WASP-32) through analyzing TESS photometry and ground-based transit observations, which were obtained at the 1-m and 2.4-m telescopes of Yunnan Observatories, China, and the 1.2-m telescope of Hamburg Observatory, Germany, as well as the data in the literature. During modelling the transit light curves, Gaussian process is employed to account for the potential systematic errors. Through comprehensive timing analysis, we find that both HAT-P-13b and HAT-P-16b show significant timing variations (TTVs) that can be explained by apsidal precession. TTVs of WASP-32b may be led by a decaying orbit due to tidal dissipation or apsidal precession. However, the current observations can not rule out the origins of three systems’ TTVs from gravitational perturbations of close planetary companions conclusively.