bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cerebral localization of chronic myelomonocytic leukemia and improvement with high‐dose cytarabine: A case report
Authors: Haméon Clémence , Machet Antoine , Drieu La Rochelle Laurianne , Vallet Nicolas , Lejeune Julien , Ravalet Noémie , Cottier Jean‐Philippe , Hérault Olivier , Gyan Emmanuel 
Published: June 2023

AbstractChronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative neoplasm characterized by the infiltration of blood and bone marrow by immature monocytes, in which extra‐hematopoietic localization is uncommon. We report the case of a 69‐year‐old‐man with highly likely ectopic brain CMML involvement by MRI. Without the possibility of cerebral biopsy and with a negative infectious disease assessment, high‐dose cytarabine‐based chemotherapy was successfully administered. The favorable evolution in this case highlights the potential benefit of such treatment, even without a cerebral biopsy to confirm the disease. This case can aid clinical decision‐making in the future.
Assessment of body composition in pediatric intestinal failure: A comparison study
Authors: Yanchis Dianna , So Stephanie , Patterson Catherine , Belza Christina , Garofalo Elizabeth , Wong‐Sterling Sylvia , Silva Carina , Avitzur Yaron , Wales Paul W. , Hulst Jessie M. , Kong Dehan , Xuyx Libai , Courtney‐Martin Glenda 
Published: June 2023

AbstractBackgroundThe objective of the study was to compare bioelectrical impedance analysis (BIA) and skinfolds with dual energy x‐ray absorptiometry (DXA) in the assessment of body composition of children with intestinal failure. DXA is the reference method for body composition assessment in clinical settings.MethodsChildren aged 1–18 years with intestinal failure whohave DXA as part of routine clinical monitoring were eligible. BIA measured total body water on the same day as DXA. Skinfold measurements were taken at four sites: triceps, biceps, subscapular, and suprailiac. Percentage of fat mass (%FM) and fat‐free mass (%FFM) were derived from resistance and reactance measured by BIA by using age‐specific equations. Percentage of FM was calculated from skinfold measures by using age‐specific equations. Data on patient characteristics, intestinal failure–related factors, and feeding method were collected. Paired t test examined differences in %FM and %FFM and Bland‐Altman analysis determined the agreement between BIA, skinfolds, and DXA. Marginal linear model assessed the effect of age, sex, and feeding method on the difference in body composition obtained between DXA and BIA and between DXA and skinfolds.ResultsSixty‐eight children with intestinal failure, mean age 8.9 ± 4.2 years, were studied. There was no difference between %FFM and %FM obtained by DXA and BIA (P = 0.26), with a mean bias (95% CI) of −0.69 (−1.9 to 0.5) for %FFM. Sex and age were individually and jointly associated with the bias observed between DXA and BIA (P < 0.05). Skinfold and DXA measurements were significantly different (P < 0.05).ConclusionsBIA is an acceptable clinical tool for assessing body composition in pediatric intestinal failure.
The combination of high‐throughput sequencing and LC–MS/MS reveals the mechanism of Staphylococcus inoculation on bacterial community succession and taste development during the processing of dry‐cured bacon
Authors: Wu Xueyi , Pan Daodong , Xia Qiang , Sun Yangying , Geng Fang , Cao Jinxuan , Zhou Changyu 
Published: June 2023

AbstractBACKGROUNDTo understand the mechanism of co‐inoculation of Staphylococcus vitulinus and Staphylococcus xylosus (SX&SV) on taste quality of dry‐cured bacon, physicochemical parameters, microbial community, metabolite compositions and taste attributes were investigated during the processing of dry‐cured bacon with Staphylococcus inoculation. The potential correlation between core bacteria and metabolites was evaluated, and the metabolic pathway of key metabolites was further explored.RESULTSThe values of pH, water activity and adhesiveness were significantly lower in SX&SV, and more than 2.56‐ and 2.15‐fold higher values in richness and overall acceptance were found in SX&SV bacon than in CK bacon. The overwhelming advantage of Staphylococcus was confirmed in SX&SV by high‐throughput sequencing. Sixty‐six metabolites were identified by liquid chromatography–tandem mass spectrometry, and oligopeptides, amino acid derivatives and organic acids were the key components. Pearson correlation demonstrated that the accumulation of oligopeptides, amino acid derivatives and organic acids were positively correlated with high abundance of Staphylococcus. The pathways of purine metabolism, glutathione metabolism and glutamate metabolism were mainly involved in developing the taste quality of SX&SV.CONCLUSIONThe co‐inoculation of Staphylococcus vitulinus and Staphylococcus xylosus enhanced the taste attributes of dry‐cured bacon. The present study provides the theoretical reference with respect to regulating the taste quality of fermented meat products by starter cultures of Staphylococcus during manufacture. © 2023 Society of Chemical Industry.
Effects of regulated deficit irrigation and foliar kaolin application on quality parameters of almond [Prunus dulcis (Mill.) D.A. Webb]
Authors: Barreales David , Fernandes Ângela , Barros Lillian , Capitão Susana , Castro Ribeiro António 
Published: June 2023

AbstractBackgroundWater stress during the growing season of the almond tree is the factor that most limits its yield. Different strategies have been studied in recent years to reduce its negative effects, such as deficit irrigation and the application of reflective spray compounds. A 3‐year experiment (2019–2021) was set in a factorial design in which the effect of regulated deficit irrigation and foliar kaolin spray was evaluated on morphological characteristics (weight, length, width, and thickness of the nut and kernel, shell thickness, kernel yield, double kernels, and damaged kernels), color properties, nutritional value (carbohydrates, fat, proteins and ash) and chemical parameters (free sugars and fatty acids profiles).ResultsIn general, the significant differences between the treatments did not have a similar trend in the 3 years of the study. Regulated deficit irrigation and kaolin had no detrimental impact on almond morphological and color characteristics. The almond free sugars concentration was relatively stable under deficit irrigation and kaolin application. On the other hand, kaolin application positively affected the synthesis of linoleic acid.ConclusionReducing the amount of irrigation water applied to almonds contributes to the sustainability of production without negatively affecting quality and even improving some quality parameters. In general, the foliar application of kaolin did not show significant differences in the evaluated morphological parameters. However, in terms of chemical composition, kaolin led to an increase in the concentration of linoleic acid and sucrose. © 2023 Society of Chemical Industry.
Efficacy of baricitinib 4 mg in patients with moderate‐to‐severe atopic dermatitis previously treated with systemic therapy
Authors: Costanzo Antonio , Tanaka Akio , Torres Tiago , Pérez‐García Bibiana , Sontag Angelina , Chen Yun‐Fei , Yang Fan Emily , Kolodsick Jill , Torisu‐Itakura Hitoe , Somani Najwa 
Published: June 2023

AbstractBackgroundThe Janus kinase (JAK)1/JAK2 inhibitor baricitinib in combination with topical corticosteroids (TCS) improved moderate‐to‐severe atopic dermatitis (AD) in the phase 3 BREEZE‐AD7 trial. Patients with previous systemic therapy may have more severe, treatment‐resistant disease.ObjectivesTo assess the efficacy of baricitinib 4 mg in adults with moderate‐to‐severe AD with and without previous use of systemic therapy.MethodsPatients were randomized 1:1:1 to TCS plus once‐daily oral placebo, 2 mg baricitinib or 4 mg baricitinib for 16 weeks. Outcomes were assessed in patients treated with baricitinib 4 mg with and without previous use of systemic therapy (N = 68, N = 43) versus placebo (N = 75, N = 34). Prior use of systemic therapy was defined as treatment of AD with oral corticosteroids, immunosuppressants or biologics. Endpoints included the proportions of patients achieving a vIGA‐AD® score 0 or 1 with >2‐point improvement from baseline, 75% improvement in the Eczema area and severity index score (EASI75), ≥4‐point improvement on the Itch numeric rating scale (NRS) and ≥4‐point improvement in the dermatology life quality index (DLQI). Logistic regression was used to evaluate consistent treatment effects across subgroups.ResultsAt Week 16, among patients with and without previous use of systemics treated with baricitinib 4 mg versus placebo, respectively, vIGA‐AD score (0, 1) was achieved in 27.9% and 34.9% versus 13.3% and 17.6%, EASI75 was met in 47.1% and 48.8% versus 22.7% and 23.5%, Itch NRS ≥ 4‐point improvement was observed in 44.4% and 43.2% versus 14.1% and 33.3% and DLQI ≥ 4‐point improvement was seen in 75.8% and 69.2% versus 52.1% and 54.8%. Consistent treatment effects were observed between patients with or without prior use of systemics (treatment‐by‐subgroup interactions p = 0.085 for Itch NRS, p > 0.1 for all others).ConclusionsBaricitinib 4 mg significantly reduced disease severity in AD compared to placebo regardless of prior systemic therapy.
Temporal Expression of Hox Genes and Phox2b in the Rat Nucleus Ambiguus During Development: Implications on Laryngeal Innervation
Authors: Honzel Emily , Hernandez‐Morato Ignacio , Joshi Abhinav , Pennington‐Fitzgerald William , Moayedi Yalda , Pitman Michael J. 
Published: June 2023

ObjectivesRecurrent laryngeal nerve (RLN) injury results in synkinetic reinnervation and vocal fold paralysis. Investigation of cues expressed in the developing brainstem that influence correct selective targeting of intrinsic laryngeal muscles may elucidate post‐injury abnormalities contributing to non‐functional reinnervation. Primary targets of interest were Hoxb1 and Hoxb2, members of the Hox family that create overlapping gradients in the developing brain, and their target Phox2b, a transcription factor necessary for cranial nerve branchio‐ and visceromotoneuron survival.MethodsRat embryos at developmental days E14, E16, E18, and E20 (4 animals/age) were sectioned for RNA in situ hybridization to detect Hoxb1, Hoxb2, and Phox2b mRNA within the brainstem. Slides were costained with Islet1 antibody for identification of the nucleus ambiguus. Results were confirmed using immunohistochemistry. Sections were imaged on a confocal microscope. RNA and protein expressions were quantified using QuPath. Statistical analyses were performed using R.ResultsHoxb1, Hoxb2, and Phox2b expressions varied according to embryologic age. Hoxb1 and Hoxb2 expression peaked at E16, with significant decreases at E18 and E20 (one‐way ANOVA p = 0.001 for both). Phox2b expression was highest at E14 and trended downward with increased embryologic age (one‐way ANOVA p = 0.005).ConclusionPeak expression of Hoxb1 and Hoxb2 is observed at time points when the RLN arrives at the larynx and begins to branch toward individual muscles, positioning these gene products to be involved in cueing laryngeal motoneuron identity and target identification. Higher expression of Phox2b earlier in development suggests a role in laryngeal motoneuron formation.Level of EvidenceNA Laryngoscope, 133:3462–3471, 2023
Cow manure compost‐based products as alternative rhizobial carrier materials
Authors: Li Yantao , Shabir Rahat , Rashti Mehran Rezaei , Megharaj Mallavarapu , Chen Chengrong 
Published: June 2023

AbstractCow manure compost (CMC) has been used widely as soil amendment to improve soil fertility and health, and to minimize environmental impacts. However, direct use of commercial CMC‐based products as alternative rhizobial carrier materials is rare, it could promote inoculant commercialization. This research aimed to explore the potential of four CMC‐based products (CMC powder, CMC granular, CMC + 50% gypsum, and CMC + 30% biochar) as a rhizobial carrier. Peat was used as a standard (control) material. All the carriers were directly mixed with Bradyrhizobium japonicum CB 1809 strain to prepare four CMC‐based inoculants. Shelf life of inoculants was evaluated up to 120 days of storage and survival rate in soil was determined after 30 days under both optimum moisture (55% WHC—water‐holding capacity) and drought stress (15% WHC) conditions. Results revealed that overall, CMC + 50% gypsum product showed better physicochemical characteristics such as WHC (126.9 ± 6.87%), Ca (134.15 ± 5.78 mg kg−1), and S (104.9 ± 6.52 mg kg−1) with the best shelf life during storage at 28°C and 35°C temperatures. The difference in shelf life between the CMC + 50% gypsum and other CMC‐based products became more apparent at higher temperature (35°C) and longer‐term storage (120 days). Additionally, when introduced into the soil, the CMC + 50% gypsum carrier outperformed the peat (control), showing the best strain survival rate of 95% (under optimum moisture) and 87% (under drought) after 30 days of storage. The strain population of CMC + 30% biochar as carrier was the lowest with values 8.66 Log 10 CFU g−1 and 7.52 Log 10 CFU g−1 in the shelf‐life test at both storage temperatures 28°C and 35°C, respectively, likely due to its alkaline pH. Multivariate analysis revealed that the carriers' WHC, Ca (involved in nodule formation and stability), and S (essential for nodule metabolism and nitrogenase activity) contents had a strong positive correlation with the long‐term shelf life and the survival rate in soil, confirming the importance of carrier materials' moisture retention and availability of specific elements (Ca, S). Hence, CMC + 50% gypsum showed the highest values of WHC, Ca and S, and optimum pH, reflecting its highest rhizobial population density and survival rate as compared to other carriers. This work has demonstrated the great potential of CMC‐based commercial products as alternative carrier materials, opening an alternative avenue for rapid commercial development of inoculants.
Laryngeal cancer treatment decision making: A conjoint analysis of general public attitudes and priorities
Authors: Upton Montana , Reddy Neha , Aker Mamdouh , James Kevin , Wang Marilene , Mendelsohn Abie H. 
Published: June 2023

AbstractObjectivePatients with advanced laryngeal cancer are typically presented with divergent treatment options, namely chemoradiation versus total laryngectomy. This study aims to understand general perspectives of the factors involved in this decision‐making process.MethodsSurveys were constructed using specialized conjoint analysis software. Seven attributes integral to the decision‐making process for advanced laryngeal cancer treatment were included.ResultsThree hundred one healthy adult volunteers completed the decision‐making program. The relative impact of each treatment attribute on decision making across all participants was scored with an average importance score (standard deviation) as follows: Lifespan 22.2% (±8.5), Voicing 21.4% (±5.9), Swallowing 19.1% (±7.3), Cancer Cure 14.9% (±6.2), Mode of Breathing 11.0% (±3.7), Self‐Image 6.7% (±2.9), and Treatment Type 4.8% (±3.0).ConclusionsGeneral public opinion ranked lifespan, voicing, and swallowing aspects as similarly important, and all were ranked more important than probability of cure. These data demonstrate a variety of priorities among participants and the need for tailored discussions when determining treatment choice for advanced laryngeal cancer.Level of EvidenceLevel 4.
Ultrahigh Precision Angular Velocity Measurement using Frequency Shift of Partially Coherent Beams
Authors: Zhao Xuechun , Wang Zhuoyi , Lu Xingyuan , Zhang Hao , Zhu Junan , Gao Jianbo , Zhan Qiwen , Cai Yangjian , Zhao Chengliang 
Published: June 2023

AbstractIn the recent decade, the rotational Doppler effect has garnered considerable attention for stimulating the development of applications such as rotational Doppler velocity and topological charge measurements. Previous studies performed measurements under sources with one or multiple amplitude, phase, and polarization modulations. However, the applicability of these schemes is limited by the crucial factor of alignment between the source and object, especially if the magnitude of the source is greater than the object size. Therefore, this study proposes a partially coherent angular velocity measurement model that allows the rotational axes of targets to deviate from the source center and is even less susceptible to external jitters. Accordingly, a proof‐of‐principle experiment to determine the angular velocity under arbitrary alignment conditions is conducted. Tracing the rotational motion by rotating the coherent structure of the source results in a frequency shift—red shift for the same rotation and blue shift for a reverse rotation. The angular velocity vectors (both magnitude and direction) of two anisotropic sub‐Rayleigh objects are successfully measured with ultrahigh precision. The lowest angular velocity is 0.001 r s−1. The average relative error is less than 0.05% with sufficient sampling. Thus, the present findings can be applied to velocity metrology and micromanipulation.
Blood immune inflammatory indicators predict prognosis in patients with coronary artery disease
Authors: Liu Ju‐E , Zheng Shufen , Chen Kai , Wang Jing , Liu Xiaoqi , Lai Weihua , Zhu Qian , Wu Zhuoyi , Meng Jinxiu , Xia Shuang , Liu Yong , Zhong Shilong 
Published: June 2023

AbstractBackgroundThe relationship between the combined hematological parameters and echocardiography and long‐term prognosis in patients with coronary artery disease (CAD) remains unclear.MethodsWe examined the ability of hematological parameters to predict all‐cause death and major adverse cardiovascular events (MACE) based on Lasso Cox regression analysis. The significant predictors of hematological parameters from the Lasso Cox model were analyzed via multivariate Cox regression analysis and by adjusting for echocardiographic data. We calculated the continuous net reclassification improvement (cNRI) and integrated discrimination improvement (IDI) of the hematological parameters to assess the improvement in prediction.ResultsA low hemoglobin and lymphocyte ratio and high hematocrit, red blood cell distribution width‐coefficient of variation, and monocyte ratio significantly increased the risk of MACE and death in CAD patients. Neutrophil‐to‐lymphocyte ratio was associated with MACE but not death in CAD patients. After adjustment for echocardiographic parameters, hemoglobin, hematocrit, and lymphocyte ratio remained independently related to death and MACE. The addition of hematological and echocardiographic parameters to the Framingham risk score model significantly improved the area under the curve of mortality (0.794 vs. 0.713, p = 0.0007) and reclassification with cNRI of 30.6% (p = 0.002) and IDI of 0.055 (p < 0.001). Mendelian randomization analyses identified that fibrinogen and neutrophil‐to‐lymphocyte ratio were associated with increased brain natriuretic peptide and decreased left ventricular ejection fraction.ConclusionsThese findings suggest that the blood immune inflammatory indicators fibrinogen and neutrophil‐to‐lymphocyte ratio were causally associated with the risk of heart failure after CAD. The combination of hematological biomarkers and echocardiography parameters as predictor variables is a useful predictive tool for all‐cause mortality in patients with CAD.
Evaluation of the clinical effects of non‐invasive prenatal screening for diseases associated with aneuploidy and copy number variation
Authors: Zhu Shaohua , Jia Chunyang , Hao Shengju , Zhang Qinghua , He Jing , Wang Xing , Lin Pengwu , Guo Yuanyuan , Li Yigang , Feng Xuan 
Published: June 2023

AbstractBackgroundTo explore and compare the clinical effects of high‐resolution non‐invasive prenatal screening (NIPS‐Plus) for common/uncommon chromosomal aneuploidy and microdeletion/microduplication syndromes (MMS).MethodsThe current prospective study included a total of 25,380 pregnant women who performed NIPS‐Plus, and amniocentesis was performed on women with MMS with the screening results to diagnose patients with suspected MMS.ResultsThere were 415 samples with positive results for NIPS‐Plus, included 275 with aneuploidy and 140 with MMS. After diagnosis by amniocentesis, 188 cases were confirmed as true positive, included46 cases of T21, 9 cases of T18, 1 case of T13, 34 cases of SCA, 41 cases of other chromosomal euploidy and 57 cases of MMS. In addition, no false negative cases were found, MMS was classified with 5 Mb with the cutoff value, and the PPV of different fragment size was counted, respectively.ConclusionWe found that the corresponding PPV was 44.66% with the fragment of copy number variation (CNV) being less than or equal to 5 Mb, and when it was greater than 5 Mb, the PPV was 29.73%, which suggested that NIPS‐Plus was more suitable for screening the PPV of small fragment abnormalities. NIPS‐Plus has a good application effect in routine aneuploidy screening and had the best detection effect for T21; moreover, it performed well in screening of MMS and had better detection effect on MMS with CNV fragment length less than 5 Mb. Based on the current results, we suggested that NIPS‐Plus should be used as a comprehensive elementary prenatal screening method for all pregnant women, but for MMS caused by abnormal large fragment CNV, the detection method and efficiency still need to be improved.
From variant of unknown significance to likely pathogenic: Characterization and pathogenicity determination of a large genomic deletion in the MLH1 gene
Authors: Bouras Ahmed , Legrand Clementine , Kourda Jihen , Ruano Eric , Grand‐Masson Chloé , Lefol Cedrick , Wang Qing 
Published: June 2023

AbstractBackgroundThe MLH1 gene is one of the DNA mismatch repair genes (MMR), implicated in Lynch syndrome (LS), an autosomal dominant hereditary tumor susceptibility disease. The advent of next‐generation sequencing (NGS) technologies has accelerated the diagnosis of inherited diseases and increased the percentage of diagnosis of inherited cancers. However, some complex genomic alterations require the combination of several analytical strategies to allow correct biological interpretations. Here, we describe a novel MLH1 deletion and its pathogenicity determination in a patient suspected of LS.MethodsThe index case was a French 73‐year‐old man diagnosed with colorectal cancer displaying microsatellite instability and the loss of MLH1 and PMS2 expression. NGS analysis was used as the primary method for MMR genes screening. Long‐range PCR and reverse transcriptase polymerase chain reaction (RT‐PCR) were used for breakpoints and pathogenicity determinations.ResultsA large genomic deletion was detected which removed the last six nucleotides of MLH1 exon 11 together with a large part of intron 11. It was initially considered as a variant of unknown significance (VUS). Genomic breakpoints were subsequently characterized defining the deletion as c.1033_1039‐248del. Further RNA analysis demonstrated that this variant activated a cryptic donor splice site at the 5′ of the breakpoint, leading to a premature truncated protein: p.Thr345Alafs*13.ConclusionOur finding suggested that although NGS technologies have increased variant detection yield, combined approaches were still needed for complex variant characterization and pathogenicity assessment.