bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automated detection, delineation and quantification of whole-body bone metastasis using FDG-PET/CT images
Authors: Nigam R. , Field M. , Harris G. , Barton M. , Carolan M. , Metcalfe P. , Holloway L. 
Published: May 2023

AbstractNon-small cell lung cancer (NSCLC) patients with the metastatic spread of disease to the bone have high morbidity and mortality. Stereotactic ablative body radiotherapy increases the progression free survival and overall survival of these patients with oligometastases. FDG-PET/CT, a functional imaging technique combining positron emission tomography (PET) with 18 F-fluorodeoxyglucose (FDG) and computer tomography (CT) provides improved staging and identification of treatment response. It is also associated with reduction in size of the radiotherapy tumour volume delineation compared with CT based contouring in radiotherapy, thus allowing for dose escalation to the target volume with lower doses to the surrounding organs at risk. FDG-PET/CT is increasingly being used for the clinical management of NSCLC patients undergoing radiotherapy and has shown high sensitivity and specificity for the detection of bone metastases in these patients. Here, we present a software tool for detection, delineation and quantification of bone metastases using FDG-PET/CT images. The tool extracts standardised uptake values (SUV) from FDG-PET images for auto-segmentation of bone lesions and calculates volume of each lesion and associated mean and maximum SUV. The tool also allows automatic statistical validation of the auto-segmented bone lesions against the manual contours of a radiation oncologist. A retrospective review of FDG-PET/CT scans of more than 30 candidate NSCLC patients was performed and nine patients with one or more metastatic bone lesions were selected for the present study. The SUV threshold prediction model was designed by splitting the cohort of patients into a subset of ‘development’ and ‘validation’ cohorts. The development cohort yielded an optimum SUV threshold of 3.0 for automatic detection of bone metastases using FDG-PET/CT images. The validity of the derived optimum SUV threshold on the validation cohort demonstrated that auto-segmented and manually contoured bone lesions showed strong concordance for volume of bone lesion (r = 0.993) and number of detected lesions (r = 0.996). The tool has various applications in radiotherapy, including but not limited to studies determining optimum SUV threshold for accurate and standardised delineation of bone lesions and in scientific studies utilising large patient populations for instance for investigation of the number of metastatic lesions that can be treated safety with an ablative dose of radiotherapy without exceeding the normal tissue toxicity.
Phase Regulation and Defect Passivation Enabled by Phosphoryl Chloride Molecules for Efficient Quasi-2D Perovskite Light-Emitting Diodes
Authors: Li Mingliang , Zhao Yaping , Guo Jia , Qin Xiangqian , Zhang Qin , Tian Chengbo , Xu Peng , Li Yuqing , Tian Wanjia , Zheng Xiaojia , Xing Guichuan , Zhang Wen-Hua , Wei Zhanhua 
Published: May 2023

Highlights

The modification of perovskite precursor by a series of phosphoryl chloride molecules can indeed improve the performance of perovskite LEDs (Pero-LEDs).

The bis(2-oxo-3-oxazolidinyl) phosphinic chloride can not only regulate the phase distribution by controlling the crystallization rate but also passivate the defects of the quasi-2D perovskite.

Highly efficient and reproducible Pero-LEDs are achieved with an maximum external quantum efficiency (EQEmax) of 20.82% and an average EQE (EQEave) of around 20% on 50 devices.

Abstract
Quasi-2D perovskites have attracted tremendous interest for application as light-emission layers in light-emitting diodes (LEDs). However, the heterogeneous n phase and non-uniform distribution still severely limit the further development of quasi-2D perovskite LEDs (Pero-LEDs). Meanwhile, the increased defect density caused by the reduced dimension and grain size induces non-radiative recombination and further deteriorates the device performance. Here, we found that a series of molecules containing phosphoryl chloride functional groups have noticeable enhancement effects on the device performance of quasi-2D Pero-LEDs. Then, we studied the modification mechanism by focusing on the bis(2-oxo-3-oxazolidinyl) phosphinic chloride (BOPCl). It is concluded that the BOPCl can not only regulate the phase distribution by decreasing the crystallization rate but also remain in the grain boundaries and passivate the defects. As a result, the corresponding quasi-2D Pero-LEDs obtained a maximum external quantum efficiency (EQEmax) of 20.82% and an average EQE (EQEave) of around 20% on the optimal 50 devices, proving excellent reproducibility. Our work provides a new selection of molecular types for regulating the crystallization and passivating the defects of quasi-2D perovskite films.
Measuring Physiological Responses to Visualizations of Urban Planning Scenarios in Immersive Virtual Reality
Authors: Keil Julian , Weißmann Marco , Korte Annika , Edler Dennis , Dickmann Frank 
Published: May 2023

AbstractStakeholder participation is an important component of modern urban planning processes. It can provide information about potential social conflicts related to specific urban planning scenarios. However, acquiring feedback from stakeholders is usually limited to explicit response types such as interviews or questionnaires. Such explicit response types are not suitable for the assessment of unconscious responses to specific parameters of an urban planning scenario. To address this limitation, we propose an approach for the assessment of affective and stress responses using implicit measures. Using a measure for electrodermal activity (EDA) and a virtual reality (VR)-based 3D urban model, we demonstrate how implicit physiological measurements can be visualized and temporally matched to specific parameters in an immersive representation of an urban planning scenario. Since this approach is supposed to support conventional stakeholder participation processes in urban planning, we designed it to be simple, cost-effective and with as little task interference as possible. Based on the additional insights gained from measuring physiological responses to urban planning scenarios, urban planners can further optimize planning scenarios by adjusting them to the derived implicitly expressed needs of stakeholders. To support simple implementation of the suggested approach, we provide sample scripts for visualization of EDA data. Limitations concerning the evaluation of raw EDA data and potentials for extending the described approach with additional physiological measures and real-time data evaluation are discussed.
Why Is It so Difficult to Investigate Violent Radicalization?
Authors: Gómez Ángel , Vázquez Alexandra , Chinchilla Juana , Blanco Laura , Alba Beatriz , Chiclana Sandra , González-Álvarez José Luis 
Published: May 2023

Abstract
Imagine that you are a researcher interested in disentangling the underlying mechanisms that motivate certain individuals to self-sacrifice for a group or an ideology. Now, visualize that you are one of a few privileged that have the possibility of interviewing people who have been involved in some of the most dramatic terrorist attacks in history. What should you do? Most investigations focused on terrorism do not include empirical data and just a handful of fortunate have made face-to-face interviews with these individuals. Therefore, we might conclude that most experts in the field have not directly met the challenge of experiencing studying violent radicalization in person. As members of a research team who have talked with individuals under risk of radicalization, current, and former terrorists, our main goal with this manuscript is to synopsize a series of ten potential barriers that those interested in the subject might find when making fieldwork, and alternatives to solve them. If all the efforts made by investigators could save the life of a potential victim, prevent an individual from becoming radicalized, or make him/her decide to abandon the violence associated with terrorism, all our work will have been worthwhile.
Verification and Model Configuration Sensitivity of Simulated ABI Radiance Forecasts With the FV3‐LAM Model
Authors: Johnson Aaron , Wang Xuguang , Blake Benjamin T. , Rogers Eric , Wang Yunheng , Carley Jacob R. , Clark Adam , Beck Jeffrey , Alexander Curtis 
Published: May 2023

AbstractThis study evaluates simulated radiance forecasts from a series of controlled experiments consisting of FV3‐LAM forecasts with different configurations of model physics and vertical resolution. The forecasts were produced during the 2020 Hazardous Weather Testbed Spring Forecasting Experiments on the same forecast cases. The evaluation includes grid‐point, neighborhood‐based and object‐based verification. The experiments include forecasts that were identical except for the physics (EMC‐LAM vs. EMC‐LAMx), vertical resolution (EMC‐LAMx vs. NSSL‐LAM), or combined initial conditions, physics and vertical resolution (GSL‐LAM). It is found that the EMC‐LAM generally provided better simulated radiance forecasts than the other three configurations at most forecast lead times, due to its unique physics configuration. All configurations generally over‐forecasted high level clouds. EMC‐LAM reduced the over‐forecasting of high clouds, but also under‐forecasted the coverage of mid‐level clouds. In contrast, at early lead times the EMC‐LAM had relatively poor performance relative to the other forecasts. Furthermore, EMC‐LAM was an outlier in terms of the vertical structure of clouds. It is also found that the NSSL‐LAM consistently improved upon the EMC‐LAMx, which had fewer vertical levels than NSSL‐LAM. Compared to EMC‐LAMx, NSSL‐LAM had less cloud over‐forecasting bias, especially with small cloud objects, and less overall error. The differences between EMC‐LAMx and GSL‐LAM were generally much smaller than the differences between EMC‐LAMx and EMC‐LAM/NSSL‐LAM. Finally, it is found that a non‐linear bias correction conditioned on symmetric brightness temperature reduced the overall root‐mean‐square error by about a factor of 2 while improving the unrealistic vertical structure of clouds in the EMC‐LAM.
Seamount Subduction and Megathrust Seismicity: The Interplay Between Geometry and Friction
Authors: Menichelli I. , Corbi F. , Brizzi S. , van Rijsingen E. , Lallemand S. , Funiciello F. 
Published: May 2023

AbstractSubducting seamounts are recognized as one of the key features influencing megathrust earthquakes. However, whether they trigger or arrest ruptures remains debated. Here, we use analog models to study the influence of a single seamount on megathrust earthquakes, separating the effect of topography from that of friction. Four different model configurations have been developed (i.e., flat interface, high and low friction seamount, low friction patch). In our models, the seamount reduces recurrence time, interseismic coupling, and fault strength, suggesting that it acts as a barrier: 80% of the ruptures concentrate in flat regions that surround the seamount and only smaller magnitude earthquakes nucleate above it. The low‐friction zone, which mimics the fluid accumulation or the establishment of fracture systems in natural cases, seems to be the most efficient in arresting rupture propagation in our experimental setting.
The Role of a Strong Out‐Of‐Plane Magnetic Field at the X‐Line in Antiparallel Magnetic Reconnection as a Guide Field
Authors: Zhang Y. C. , Wang C. , Dai L. , Tang S. Y. , Burch J. L. 
Published: May 2023

AbstractThe magnetic reconnection process substantially changes the configuration of the magnetic field in plasma and explosively releases magnetic energy. The asymptotic magnetic field lines during magnetic reconnection events can be strictly antiparallel or only have one antiparallel component, and the magnetic reconnection features are distinct under different asymptotic magnetic fields. Here, we provide the first observational evidence demonstrating the existence of a strong out‐of‐plane magnetic field at the X‐line in antiparallel reconnection. The analyses described herein show that this strong out‐of‐plane magnetic field significantly changes the structure of the diffusion region, thereby altering the reconnection features in terms of particle dynamics and energy dissipation by way of a guide field. A new pattern of the normal electric field near the X‐line is responsible for producing this out‐of‐plane magnetic field. Our findings suggest that, in addition to asymptotic conditions, local processes inside the diffusion region can also determine whether magnetic reconnection events develop as antiparallel reconnection or component reconnection.
Glia maturation factor beta deficiency protects against diabetic osteoporosis by suppressing osteoclast hyperactivity
Authors: Shi Si , Gu Huijie , Xu Jinyuan , Sun Wan , Liu Caiyin , Zhu Tong , Wang Juan , Gao Furong , Zhang Jieping , Ou Qingjian , Jin Caixia , Xu Jingying , Chen Hao , Li Jiao , Xu Guotong , Tian Haibin , Lu Lixia 
Published: May 2023

AbstractExcessive osteoclast activation, which depends on dramatic changes in actin dynamics, causes osteoporosis (OP). The molecular mechanism of osteoclast activation in OP related to type 1 diabetes (T1D) remains unclear. Glia maturation factor beta (GMFB) is considered a growth and differentiation factor for both glia and neurons. Here, we demonstrated that Gmfb deficiency effectively ameliorated the phenotype of T1D-OP in rats by inhibiting osteoclast hyperactivity. In vitro assays showed that GMFB participated in osteoclast activation rather than proliferation. Gmfb deficiency did not affect osteoclast sealing zone (SZ) formation but effectively decreased the SZ area by decreasing actin depolymerization. When GMFB was overexpressed in Gmfb-deficient osteoclasts, the size of the SZ area was enlarged in a dose-dependent manner. Moreover, decreased actin depolymerization led to a decrease in nuclear G-actin, which activated MKL1/SRF-dependent gene transcription. We found that pro-osteoclastogenic factors (Mmp9 and Mmp14) were downregulated, while anti-osteoclastogenic factors (Cftr and Fhl2) were upregulated in Gmfb KO osteoclasts. A GMFB inhibitor, DS-30, targeting the binding site of GMFB and Arp2/3, was obtained. Biocore analysis revealed a high affinity between DS-30 and GMFB in a dose-dependent manner. As expected, DS-30 strongly suppressed osteoclast hyperactivity in vivo and in vitro. In conclusion, our work identified a new therapeutic strategy for T1D-OP treatment. The discovery of GMFB inhibitors will contribute to translational research on T1D-OP.
The role of the genomic mutation signature and tumor mutation burden on relapse risk prediction in head and neck squamous cell carcinoma after concurrent chemoradiotherapy
Authors: Wang Hui-Ching , Moi Sin-Hua , Chan Leong-Perng , Wu Chun-Chieh , Du Jeng-Shiun , Liu Pei-Lin , Chou Meng-Chun , Wu Che-Wei , Huang Chih-Jen , Hsiao Hui-Hua , Pan Mei-Ren , Chen Li-Tzong 
Published: May 2023

AbstractPersonalized genetic profiling has focused on improving treatment efficacy and predicting risk stratification by identifying mutated genes and selecting targeted agents according to genetic testing. Therefore, we evaluated the role of genetic profiling and tumor mutation burden (TMB) using next-generation sequencing in patients with head and neck squamous cell carcinoma (HNSC). The relapse mutation signature (RMS) and chromatin remodeling mutation signature (CRMS) were explored to predict the risk of relapse in patients with HNSC treated with concurrent chemoradiotherapy (CCRT) with platinum-based chemotherapy. Patients in the high RMS and CRMS groups showed significantly shorter relapse-free survival than those in the low RMS and CRMS groups, respectively (p < 0.001 and p = 0.006). Multivariate Cox regression analysis showed that extranodal extension, CCRT response, and three somatic mutation profiles (TMB, RMS, and CRMS) were independent risk predictors for HNSC relapse. The predictive nomogram showed satisfactory performance in predicting relapse-free survival in patients with HNSC treated with CCRT.
Mesenchymal stem cell-derived extracellular vesicles protect against abdominal aortic aneurysm formation by inhibiting NET-induced ferroptosis
Authors: Chen Liang , Liu Yuting , Wang Zheyu , Zhang Leiyang , Xu Yi , Li Yinan , Zhang Lan , Wang Guiming , Yang Shuofei , Xue Guanhua 
Published: May 2023

AbstractNeutrophil extracellular traps (NETs) play an important role in abdominal aortic aneurysm (AAA) formation; however, the underlying molecular mechanisms remain unclear. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) may exert therapeutic effects on AAA through their immunomodulatory and regenerative abilities. This study aimed to examine the role and mechanism of MSC-EVs in regulating the development of NET-mediated AAA. Excessive release of NETs was observed in patients with AAA, and the levels of NET components were associated with the clinical outcomes of the patients. Datasets from the Gene Expression Omnibus database were analyzed and revealed that the PI3K/AKT pathway and ferroptosis were strongly associated with NETosis during AAA formation. Further experiments verified that NETs promoted AAA formation by inducing ferroptosis in smooth muscle cells (SMCs) by inhibiting the PI3K/AKT pathway. The PI3K agonist 740 Y-P, the ferroptosis inhibitor ferrostatin-1, and Padi4 deficiency significantly prevented AAA formation. MSC-EVs attenuated AAA formation by reducing NET release in an angiotensin II-induced AAA mouse model. In vitro experiments revealed that MSC-EVs reduced the release of NETs by shifting NETosis to apoptosis. Our study indicates an important role for NET-induced SMC ferroptosis in AAA formation and provides several potential targets for AAA treatment.
Epigenetic regulation of SMAD3 by histone methyltransferase SMYD2 promotes lung cancer metastasis
Authors: Kim Kwangho , Ryu Tae Young , Jung Eunsun , Han Tae-Su , Lee Jinkwon , Kim Seon-Kyu , Roh Yu Na , Lee Moo-Seung , Jung Cho-Rok , Lim Jung Hwa , Hamamoto Ryuji , Lee Hye Won , Hur Keun , Son Mi-Young , Kim Dae-Soo , Cho Hyun-Soo 
Published: May 2023

AbstractEpigenetic alterations, especially histone methylation, are key factors in cell migration and invasion in cancer metastasis. However, in lung cancer metastasis, the mechanism by which histone methylation regulates metastasis has not been fully elucidated. Here, we found that the histone methyltransferase SMYD2 is overexpressed in lung cancer and that knockdown of SMYD2 could reduce the rates of cell migration and invasion in lung cancer cell lines via direct downregulation of SMAD3 via SMYD2-mediated epigenetic regulation. Furthermore, using an in vitro epithelial-mesenchymal transition (EMT) system with a Transwell system, we generated highly invasive H1299 (In-H1299) cell lines and observed the suppression of metastatic features by SMYD2 knockdown. Finally, two types of in vivo studies revealed that the formation of metastatic tumors by shSMYD2 was significantly suppressed. Thus, we suggest that SMYD2 is a potential metastasis regulator and that the development of SMYD2-specific inhibitors may help to increase the efficacy of lung cancer treatment.
Clinical molecular subtyping reveals intrinsic mesenchymal reprogramming in gastric cancer cells
Authors: Jang Eunji , Shin Min-Kyue , Kim Hyunki , Lim Joo Yeon , Lee Jae Eun , Park Jungmin , Kim Jungeun , Kim Hyeseon , Shin Youngmin , Son Hye-Young , Choi Yoon Young , Hyung Woo Jin , Noh Sung Hoon , Suh Jin-Suck , Sung Ji-Yong , Huh Yong-Min , Cheong Jae-Ho 
Published: May 2023

AbstractThe mesenchymal cancer phenotype is known to be clinically related to treatment resistance and a poor prognosis. We identified gene signature-based molecular subtypes of gastric cancer (GC,n = 547) based on transcriptome data and validated their prognostic and predictive utility in multiple external cohorts. We subsequently examined their associations with tumor microenvironment (TME) features by employing cellular deconvolution methods and sequencing isolated GC populations. We further performed spatial transcriptomics analysis and immunohistochemistry, demonstrating the presence of GC cells in a partial epithelial-mesenchymal transition state. We performed network and pharmacogenomic database analyses to identify TGF-β signaling as a driver pathway and, thus, a therapeutic target. We further validated its expression in tumor cells in preclinical models and a single-cell dataset. Finally, we demonstrated that inhibition of TGF-β signaling negated mesenchymal/stem-like behavior and therapy resistance in GC cell lines and mouse xenograft models. In summary, we show that the mesenchymal GC phenotype could be driven by epithelial cancer cell-intrinsic TGF-β signaling and propose therapeutic strategies based on targeting the tumor-intrinsic mesenchymal reprogramming of medically intractable GC.
Crystal structure of LRG1 and the functional significance of LRG1 glycan for LPHN2 activation
Authors: Yang Jimin , Yin Guo Nan , Kim Do-Kyun , Han Ah-reum , Lee Dong Sun , Min Kwang Wook , Fu Yaoyao , Yun Jeongwon , Suh Jun-Kyu , Ryu Ji-Kan , Kim Ho Min 
Published: May 2023

AbstractThe serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling. Serum LRG1 and neutrophil-derived LRG1 have different molecular weights due to differences in glycosylation, but the impact of the differential glycan composition in LRG1 on its cellular function is largely unknown. We previously reported that LRG1 can promote both angiogenic and neurotrophic processes under hyperglycemic conditions by interacting with LPHN2. Here, we determined the crystal structure of LRG1, identifying the horseshoe-like solenoid structure of LRG1 and its four N-glycosylation sites. In addition, our biochemical and cell-biological analyses found that the deglycosylation of LRG1, particularly the removal of glycans on N325, is critical for the high-affinity binding of LRG1 to LPHN2 and thus promotes LRG1/LPHN2-mediated angiogenic and neurotrophic processes in mouse tissue explants, even under normal glucose conditions. Moreover, the intracavernous administration of deglycosylated LRG1 in a diabetic mouse model ameliorated vascular and neurological abnormalities and restored erectile function. Collectively, these data indicate a novel role of LRG1 glycans as molecular switches that can tune the range of LRG1’s cellular functions, particularly the LRG1/LPHN2 signaling axis.