bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterotypic interaction promotes asymmetric division of human hematopoietic stem and progenitor cells
Authors: Candelas Adrian , Vianay Benoit , Gelin Matthieu , Faivre Lionel , Larghero Jerome , Blanchoin Laurent , Théry Manuel , Brunet Stephane 
Published: September 2023

AbstractHematopoietic Stem and Progenitor Cells (HSPCs) give rise to all cell types of the hematopoietic system through various processes including asymmetric divisions. However, the contribution of stromal cells of the hematopoietic niches in the control of HSPC asymmetric divisions remains unknown. Using polyacrylamide microwells as minimalist niches, we show that heterotypic interaction with osteoblast promotes asymmetric division of human HSPC. Upon interaction, HSPCs polarize in interphase with centrosome, the Golgi apparatus and lysosomes positioned close to the site of contact. Subsequently during mitosis, HSPCs orient their spindle perpendicular to the plane of contact. This division gives rise to siblings with unequal amounts of lysosomes and differentiation markers such as CD34. Such asymmetric inheritance generates heterogeneity in the progeny, which is likely to be a key contributor to the plasticity of the early steps of hematopoiesis.
Complex-specific subunits of endosomal tethering factors determine the balance between HOPS/CORVET complexes
Authors: Sőth Ármin , Molnár Márton , Lőrincz Péter , Simon-Vecsei Zsófia , Juhász Gábor 
Published: September 2023

AbstractThe endolysosomal tethering complexes HOPS and CORVET play pivotal roles in the homo- and heterotypic fusion of early and late endosomes, respectively, and HOPS also mediates the fusion of lysosomes with incoming vesicles including late endosomes and autophagosomes. These heterohexameric complexes share their four core subunits that assemble with additional two, complex-specific subunits. These features and the similar structure of the complexes allow the formation of hybrid complexes, and the complex specific subunits may compete for binding to the core. In our study, we decided to gain insight into how human HOPS and CORVET complexes form. We found that the overexpression of CORVET-specific Vps8 or Tgfbrap1 decreased the amount of core proteins Vps11 and Vps18 that are assembled with HOPS-specific subunits Vps41 or Vps39, which suggests reduced amount of intracellular HOPS. In line with this, we observed that the level of lipidated LC3 protein was elevated in these cells and the autophagic cargo p62 showed accumulation in cells overexpressing Vps8, suggesting failure in autophagosome-lysosome fusion in line with loss of HOPS function. In contrast, overexpression of HOPS-specific Vps39 or Vps41 did not affect the levels of these autophagic markers. Finally, we found that a hybrid complex containing Vps39 and Vps8 could be formed in HEK293 cells.
Comparative omics reveals unanticipated metabolic rearrangements in a high-oil mutant of plastid acetyl-CoA carboxylase
Authors: Kataya Amr , Nascimento Jose Roberto S. , Xu Chunhui , Garneau Matthew G. , Koley Somnath , Kimberlin Athen , Mooney Brian P. , Allen Doug K. , Bates Philip D. , Koo Abraham J. , Xu Dong , Thelen Jay J. 
Published: September 2023

ABSTRACTHeteromeric acetyl-CoA carboxylase (ACCase) catalyzes the ATP-dependent carboxylation of acetyl-CoA to produce malonyl-CoA, the committed step forde novofatty acid synthesis. In plants, ACCase activity is controlled at multiple levels, including negative regulation by biotin attachment domain-containing (BADC) proteins, of which thebadc1/3double mutant leads to increased seed triacylglycerol accumulation. Unexpectedly, the Arabidopsisbadc1/3mutant also accumulates more protein. The metabolic consequences from both higher oil and protein was investigated in developingbadc1/3seed using global transcriptomics, translatomics, proteomics, and metabolomics. Changes include: reduced plastid pyruvate dehydrogenase; increased acetyl-CoA synthetase; increased storage and lipid-droplet packaging proteins; increased lipases; and increased β-oxidation fatty acid catabolism. We present a model of how Arabidopsis adapted to deregulated ACCase, limiting total oil accumulation, and altering flux through pathways of carbon accumulation that presents possible targets for future bioengineering of valuable seed storage reserves.
Anionic phospholipids determine G protein coupling selectivity at distinct subcellular membrane compartments
Authors: Hernandez Evelyn H. , Manglik Aashish , Irannejad Roshanak 
Published: September 2023

ABSTRACTG protein-coupled receptors (GPCRs) activate different signaling pathways through coupling to heterotrimeric G proteins consisting of four types of G alpha (Gα) subunits and the beta gamma (ýy) subunits. The C-terminal regions of Gα proteins is thought to determine coupling selectivity. However, there are several reports indicating that some receptors are promiscuous and can couple to multiple Gα proteins. The precise mechanism promoting promiscuous coupling is not fully understood. Here we show that anionic phospholipids such as phosphoinositide 4,5 bisphosphate (PI(4,5)P2) promote electrostatic interactions between anionic lipid head groups and positively charged amino acids in the transmembrane 4 (TM4) region of beta adrenergic receptors (βARs) to prime receptor coupling to the cognate Gαs and non-cognate Gαi proteins. The promiscuous coupling can only occur at the plasma membrane, a membrane compartment enriched in PI(4,5)P2, while receptors only couple to cognate Gαs proteins in compartments that are enriched in less negatively charged phosphoinositides such PI4P at the Golgi membranes. We took advantage of the rapamycin dimerization system to rapidly and inducibly deplete PI(4,5)P2 by recruiting a phosphatase to the plasma membrane and found that in the absence of anionic phospholipids, βARs couple only to their cognate Gαs protein. Finally, we found that mutating βARs PI(4,5)P2 binding motifs or depleting PI(4,5)P2 results in enhanced βARs-mediated cAMP response. Together, these findings reveal a role for anionic phosphoinositides in regulating GPCR activity at different subcellular compartments.
Definitions of digital biomarkers: a systematic mapping of the biomedical literature
Authors: Alonso Ana Karen Macias , Hirt Julian , Woelfle Tim , Janiaud Perrine , Hemkens Lars G. 
Published: September 2023

ABSTRACTBackgroundTechnological devices such as smartphones, wearables, sensors, or virtual assistants allow to collect data on health and disease processes and are thus increasingly considered a useful digital alternative to conventional biomarkers. We aimed to provide a systematic overview of the emerging literature on “digital biomarkers” with their definitions, features, and citations in biomedical research.MethodsWe analyzed all articles in PubMed that used the term “digital biomarker(s)” in title or abstract, considering any study involving humans and any review, editorial, perspective, or other opinion-based article up to 8 March 2023. We systematically extracted characteristics of publications and research studies, and any definitions and features of “digital biomarkers” mentioned. We described the most influential literature on digital biomarkers and their definitions using thematic categorizations of definitions considering the FDA BEST framework (i.e., data type, data collection method, purpose of biomarker), analysing the structural similarity of definitions by performing text analyses (hierarchical clustering on the distance-matrix) and citation analyses (based on citation metrics obtained from OpenAlex via Local Citation Network; last search 26 June 2023).FindingsWe identified 415 articles prominently using the term “digital biomarker”. They were published between 2014 and 2023 (median 2021), mostly describing primary research (283 articles; 68%). Most articles did not provide a definition of a digital biomarker (n=287; 69%). The 128 articles providing a definition of a digital biomarker reported 127 different definitions. Of these 127 definitions, 78 considered data collection, 56 data type, 50 the purpose, and 23 were based on all three key components. The 128 articles with a definition were cited a median of 6 times (interquartile range 2-20) with up to 517 citations. Of the ten most frequently cited articles using a definition, all used a different one.InterpretationThe most frequently used definitions for digital biomarkers are highly different and there is no consensus about what this emerging term means. Our overview highlights key defining characteristics of digital biomarkers which can inform the development of a harmonized and more widely accepted definition.FundingNo specific funding.
Distinct genomic signatures and modifiable risk factors underly the comorbidity between major depressive disorder and cardiovascular disease
Authors: Bergstedt Jacob , Pasman Joëlle A. , Ma Ziyan , Harder Arvid , Yao Shuyang , Parker Nadine , Treur Jorien L. , Smit Dirk J.A. , Frei Oleksandr , Shadrin Alexey , Meijsen Joeri J. , Shen Qing , Hägg Sara , Tornvall Per , Buil Alfonso , Werge Thomas , Hjerling-Leffler Jens , Als Thomas D. , Børglum Anders D. , Lewis Cathryn M. , McIntosh Andrew M. , Valdimarsdóttir Unnur A. , Andreassen Ole A. , Sullivan Patrick F. , Lu Yi , Fang Fang 
Published: September 2023

AbstractMajor depressive disorder (MDD) and cardiovascular disease (CVD) are often comorbid, resulting in excess morbidity and mortality. Using genomic data, this study elucidates biological mechanisms, key risk factors, and causal pathways underlying their comorbidity. We show that CVDs share a large proportion of their genetic risk factors with MDD. Multivariate genome-wide association analysis of the shared genetic liability between MDD and atherosclerotic CVD (ASCVD) revealed seven novel loci and distinct patterns of tissue and brain cell-type enrichments, suggesting a role for the thalamus. Part of the genetic overlap was explained by shared inflammatory, metabolic, and psychosocial/lifestyle risk factors. Finally, we found support for causal effects of genetic liability to MDD on CVD risk, but not from most CVDs to MDD, and demonstrated that the causal effects were partly explained by metabolic and psychosocial/lifestyle factors. The distinct signature of MDD-ASCVD comorbidity aligns with the idea of an immunometabolic sub-type of MDD more strongly associated with CVD than overall MDD. In summary, we identify plausible biological mechanisms underlying MDD-CVD comorbidity, as well as key modifiable risk factors for prevention of CVD in individuals with MDD.
Latent Classes of Anthropometric Growth in Early Childhood Using Uni- and Multivariate approaches in a South African Birth Cohort
Authors: van Biljon Noëlle , Lake Marilyn T , Goddard Liz , Botha Maresa , Zar Heather J , Little Francesca 
Published: September 2023

AbstractBackgroundConventional methods for modelling longitudinal growth data focus on the analysis of mean longitudinal trends or the identification of abnormal growth based on cross-sectional standardized z-scores. Latent Class Mixed Modelling (LCMM) considers the underlying heterogeneity in growth profiles and allows for the identification of groups of subjects that follow similar longitudinal trends.MethodsLCMM was used to identify underlying latent profiles of growth for univariate responses of standardized height, standardized weight, standardized body mass index and standardized weight-for-length/height measurements and multivariate response of joint standardized height and standardized weight measurements from birth to five years for a sample of 1143 children from a South African birth cohort, the Drakenstein Child Health Study (DCHS). Allocations across latent growth classes were compared to better understand the differences and similarities across the classes identified given different composite measures of height and weight as input.ResultsFour classes of growth within standardized height (n1=516, n2=112, n3=187, n4=321) and standardized weight (n1=263, n2=150, n3=584, n4=142), three latent growth classes within Body Mass Index (BMI) (n1=481, n2=485, n3=149) and Weight for length/height (WFH) (n1=321, n2=710, n3=84) and five latent growth classes within the multivariate response of standardized height and standardized weight (n1=318, n2=205, n3=75, n4=296, n5=242) were identified, each with distinct trajectories over childhood. A strong association was found between various growth classes and abnormal growth features such as rapid weight gain, stunting, underweight and overweight.ConclusionsWith the identification of these classes, a better understanding of distinct childhood growth trajectories and their predictors may be gained, informing interventions to promote optimal childhood growth.Key MessagesFour latent classes of growth were identified within standardized height and standardized weight.Three latent classes of growth were identified within standardized body mass index and standardized weight-for-length/height.Five latent classes of growth were identified within a multivariate response of standardized height and standardized weight.Latent classes identified using various composite measures of standardized height and standardized weight (standardized body mass index and standardized weight-for-length/height and a multivariate response of standardized height and standardized weight) were distinct, reiterating the benefit of examining each outcome.A strong association was found between various growth classes and abnormal growth features such as rapid weight gain, stunting, underweight and overweight.
Nepenthespitchersversusthermogenic flowers: Thermal patterns and their role in prey capture and pollination
Authors: Sujatha Gokul Baburaj , Johnson Anil John , Hussain Abdul Azeez , Baby Sabulal 
Published: September 2023

AbstractPrey capture inNepenthesand pollination in angiosperms are two antithetical events; one designed to trap insects and other arthropods (carnivory) and the other to transfer pollen through pollinators (reproduction). In this study infrared thermography is extensively used to obtain thermal profiles ofNepenthespitchers and thermogenic flowers in field conditions.N. khasianapitchers displayed below ambient temperatures during evening-night-morning hours (5 pm to 8-9-10 am); pitcher spots recorded lowest and highest temperatures as 14.4 (6-7 am) and 45.6°C (2 pm), respectively. In the evening-night-morning hours top pitcher spots displayed significant decrease (upto 6.7°C) from the ambient temperature. The average humidities in the night and day periods were 77.15% and 50.15%, respectively. Thermographic tracking of the pitcher (lid) opening inN. khasianademonstrated initial ‘wet’ pitcher in night, which gradually switched to a relatively dry surface in the morning-day times; but the peristome region retained the ‘wetness’ till 10.30 a.m. The prey capturing zones inNepenthespitchers (peristome, lid and their intersection) are colder favoring prey capture, whereas in thermogenic flowers, floral portions are hotter, providing the thermal requirements for the pollinator. In thermogenic plants, floral zones assist pollination by offering a ‘thermal reward’ through enzymatic processes; butNepenthestraps achieve lower temperature spots by physical (surface microstructures), chemical (extrafloral nectar) and ecological (rain, humidity) factors.
Is your company competent, interpersonal or community focused? The effect of values and brand portfolio on company reputation
Authors: Katsanis Lea Prevel , Williams Alan , Srirangan Kajan 
Published: September 2023

Purpose
The purpose of this study is twofold: first, to determine if pharmaceutical companies can be grouped based on their espoused values, and second, to examine the relationship between these values and company reputation.

Design/methodology/approach
A descriptive study design is used with two separate analyses: cluster analysis for grouping the companies; and descriptive data analysis for determining cluster differences.

Findings
The findings suggest that there are three value clusters: competent, community and interpersonal, with the community group showing the highest relative reputation, and the interpersonal cluster as the lowest. Brand portfolio composition appears to positively contribute to reputation. The effect of portfolio specialization is based on a company’s closeness to its therapeutic community, which may be influenced by the outward characteristics of its values.

Research limitations/implications
Future research should examine the longitudinal effects of values on reputation combined with case studies.

Practical implications
Regardless of cluster classification, all firms should develop strong ties with their therapeutic communities using both personal and digital/omnichannel strategies.

Social implications
A company’s values are becoming an important consideration for all customers and stakeholders.

Originality/value
To the best of the authors’ knowledge, this study is the first to systematically examine the activities of leading pharmaceutical firms to link a specific value cluster to company reputation.
How does digital transformation affect innovation in knowledge-intensive business services firms? The moderating effect of R&D collaboration portfolio
Authors: Duan Yunlong , Yang Meng , Liu Hanxiao , Chin Tachia 
Published: September 2023

Purpose
Firms are driven to ride on the digital wave in today’s open innovation ecosystem. This study aims to explore the effect of digital transformation (DT) on knowledge-intensive business services (KIBS) firms’ innovation ambidexterity, namely, radical versus incremental innovation, respectively. Meanwhile, the authors evaluated the moderating role of the complexity of R&D collaboration portfolio (i.e. organizational diversity and geographic diversity) in the above relationships.

Design/methodology/approach
Using a panel data set of 171 Chinese listed firms in the information and communications technology services industry from 2010 to 2018, the proposed hypotheses were empirically attested.

Findings
It is found that DT has a positive relationship with radical innovation and an inverted U-shaped relationship with incremental innovation. In terms of the R&D collaboration portfolio, organizational diversity positively moderates the relationships between DT and innovation ambidexterity, respectively. The geographic diversity weakens the inverted U-shaped effect of DT on incremental innovation; however, its moderating role in the link between DT and radical innovation is not empirically verified.

Originality/value
Extant scholars mainly addressed the interplay between KIBS firms and their manufacturing clients, while this study reveals the different consequences of DT on KIBS firms’ innovation ambidexterity to highlight the role of KIBS firms is an independent and essential innovator in a knowledge-driven economy. Notably, the findings contribute to knowledge management (KM) and R&D literature by confirming the diversity of the R&D collaboration portfolio is a critical KM strategy for KIBS firms to develop and promote external knowledge resources.
Moderating effect of gender on service convenience and customer satisfaction: an empirical study of Indian e-retailers
Authors: Shamsi Sheeraz , Khan Sablu , Khan Mohd Afaq 
Published: September 2023

PurposeThe present study has been carried out to assess the effect of constructs of service convenience on customer satisfaction of the Indian online shoppers.Design/methodology/approachThe primary data was collected through a structured questionnaire. Convenience sampling has been used to choose a sample (n = 260) of e-shoppers in India. Factor analyses (both EFA and CFA) have been done to validate different factors and its items. A conceptual model has been proposed to measure the effect of different factors of service convenience on customer satisfaction. Moreover, the perceived difference with respect to study variables has been measured. The path analysis through AMOS 22.0 has been done to test the hypotheses under study.FindingsIt can be concluded that the effect of access convenience, search convenience, and order convenience have significant effects on customer satisfaction. However, evaluation convenience and logistics and reverse logistics convenience have an insignificant effect on customer satisfaction. The present study has a unique contribution in the field of service convenience to e-retailing customers. Moreover, the present study indicates that gender does not moderate the effect of convenience on customer satisfaction.Originality/valueThis is one of the few papers that focuses solely on the effect of gender on service convenience and customer satisfaction. The findings will generate value with their originality and significant managerial implications for marketers, as well as future research directions for the researchers.
Greenwashing: a rapid escape from sustainability or a slow transition?
Authors: Yildirim Seda 
Published: September 2023

PurposeSome wrong methods have emerged in the form of greenwashing while on the way to becoming a green and sustainable brand or business. Recently, it has been questioned whether businesses or brands act by their green image. This study aims to determine whether greenwashing practices represent a slow transition to sustainability or a rapid escape.Design/methodology/approachThis study used secondary data sources and reviewed collected secondary sources. It was a qualitative study due to its methodology. The findings were mostly based on the author’s assumptions through review results.FindingsAs a result of the review of the literature and current sources, it was determined that there are two sides of greenwashing: a dark side and a white side. The dark side of greenwashing represented a rapid escape from sustainability and the white side of greenwashing represented a slow transition to sustainability. Positive and negative elements were summarized in the study and the study gave an original model showing two sides of greenwashing.Research limitations/implicationsThis study provides a viewpoint on greenwashing. This study is limited with its review results and used secondary data. Different approaches or different studies can explore different results for the related literature.Practical implicationsThe purpose of this study is not to justify companies or brands employing greenwashing. However, for a sustainable future, the contribution of manufacturers, businesses and brands as the supply side has a vital value. Therefore, it is recommended to criticize greenwashing by encouraging brands and companies that are trying to take more positive steps in the transition to sustainability.Social implicationsPolicymakers and individuals can improve the dark side of greenwashing. The biggest motive for sustainable production is the demand for green consumers. Accordingly, firms and brands will be sustainable and green in the long term in a good way as consumers and policymakers guide them in a good way.Originality/valueThis study gives an alternative view of greenwashing by comparing the dark side with the white side. The literature mostly expressed that greenwashing had bad implications but this study tried to find the good sides of greenwashing.