bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of Heilongjiang Nanwenghe Nature Reserve in Improving Habitat Quality in and around the Reserve
Authors: Li Daozheng , Liang Diling , Fakheran Sima , Li Tongning , Mumuni Joseph , Shrestha Anil , Sunderland Terry 
Published: April 2023

Abstract
Biodiversity loss is a critical challenge globally, and protected areas (PAs) has been established as an important policy tool for conservation. However, doubts exist regarding their effectiveness, and their policy effects and spatial spillover effects on surrounding areas are poorly understood. To address this, this study evaluated the effectiveness of Heilongjiang Nanwenghe National Nature Reserve (HNNNR) in China by using a combination of the InVEST model and the improved SDID model. The study covers a time span of approximately 31 years (1990–2020) and is divided into two periods (1990–1999 and 1999–2020), which allows for the assessment of the effects of nature reserves in the region. Our results showed that: (1) The establishment of HNNNR has improved the habitat quality in the reserve and Non-reserve area, with a greater impact on habitat quality in non-reserve areas than in the reserve; (2) The core zone within HNNNR showed the most significant improvement in habitat quality, while the buffer zone showed the least improvement; (3) The improvement of habitat quality in non-reserve area was mainly contributed by the policy spatial spillover effects, where the buffer zone has the strongest spillover benefits to the non-reserve, but the core zone has the weakest spillover effects to the non-reserve. Our results show the beneficial impact of a nature reserve for improving habitat quality in and around the reserve. This study provides a quantitative paradigm for assessing the conservation effectiveness of PAs across temporal and spatial scales.
XRCC1 gene rs72484243 polymorphism is associated with increased laryngeal cancer risk
Authors: Alimu Nilipaer , Maimaiti Aierpati , Maimaitituerxun Mulading , Muertizha Halimulati , Qukuerhan Ayiheng , Yasheng Yalikun , Yong Jun , Mierzhamu Nuerbiya , Mierzhakemu Muzhapaier , Aierken Aierpati , Abulaiti Aimitaji 
Published: April 2023

Abstract

Background The focus of this research is to discuss whether genetic polymorphisms in the DNA repair gene XRCC1 are linked to laryngeal cancer in patients. Methods In total 120 individuals, comprising 60 patients with Laryngeal squamous cell carcinoma (LSCC) and 60 healthy volunteers, participated in the present research. Blood samples were taken and analyzed and four XRCC1 polymorphisms (rs145135970, rs1799780, rs25489, and rs72484243) were genotyped. Results Sex, age, BMI, and smoking were shown to be the high-risk factors in the LSCC populations. Genotype and allele distributions for rs145135970, rs1799780, rs25489, and rs72484243 differed significantly between LSCC and control groups (allP < 0.05). Furthermore, carriers with the rs72484243 GTGT- allele exhibited an increased risk of LSCC relative to those who had the rs145135970 GTGTGTGTGTGTGT- allele, the rs1799780 G-A allele, or the rs25489 C-T allele, as determined by binary logistic regression analysis (OR = 2.74, 95% CI: 1.27–5.91, P = 0.01), after accounting for possible confounders like sex, age, BMI, drinking and smoking behavior, and special diet requirements. In addition, a TA haplotype and a GTGTGTGTGTGTGTTG haplotype were linked to LSCC in Chinese populations in a haploid association study of four SNP loci in the XRCC1 gene (P = 0.05;OR = 1.36, 95%CI = 1.1228–1.6406). Conclusions A large-scale population-based investigation is strongly recommended since rs72484243 gene variations are linked to an elevated risk of LSCC.
A negative feedback on El Niño events caused by extreme fire activity in equatorial Asia
Authors: Kasoar Matthew , Bayley Clara , Wells Christopher , Voulgarakis Apostolos 
Published: April 2023

Abstract

Large peatland fires in equatorial Asia during recent El Niño years have resulted in widespread smoke pollution, with record-breaking impacts on air quality and carbon emissions. Here we show that large fires during El Niño years also create a negative feedback on El Niño intensity and regional teleconnections. By comparing climate model simulations using prescribed fire emissions from a strong El Niño event versus from a non-El Niño year, we show that El Niño-enhanced fire emissions result in large surface cooling and atmospheric heating of up to 40 Wm− 2 over much of equatorial Asia. This atmospheric heating shifts cloud cover westward in the Pacific, increasing cloud amount and precipitation over equatorial Asia, and strengthening the tropical Walker circulation. This opposes the typical El Niño circulation in the Pacific, and drives a sizeable reduction in peak sea surface temperature anomalies over the Niño 3.4 region by around 0.4 K, or 22%.
Genetic Variants of Vitamin D, Estrogen α, Parathyroid and Collagen Type I alpha Receptor Gene and its Influence on Circulating Serum Osteocalcin in Postmenopausal Osteoporosis: A Cohort Study
Authors: Fernandez Chrisanne , JebasinghTennyson  , S Priscilla A 
Published: April 2023

Abstract

Introduction: Osteocalcin is an abundant, highly conserved bone specific protein and the serum levels of OC have been used as a biochemical marker of bone turnover. The genetic variation of certain candidate genes impacts osteocalcin levels in the postmenopausal period and may predispose some women to high bone turnover. To identify the genes influencing variation in serum OC levels, we investigated the polymorphisms of Vitamin D, Estrogen α, Parathyroid and Collagen Type I alpha Receptor genes and its association with bone turnover evaluated by serum osteocalcin in postmenopausal women from south India.
Materials and Methods: The polymerase chain reaction-restriction fragment length polymorphism strategy was used to detect the polymorphisms at all the four gene receptors (i.e., for VDR, ERα, PTH and COLIA1) in 300 postmenopausal women from South India. Serum osteocalcin levels were measured by immunoassay (ELISA).
Results: The serum osteocalcin levels for the Apa I polymorphisms showed varied results, in which, subjects in the control group with “GG” genotype and the osteopenic group with “TT” genotype of the ApaI polymorphism had a significantly higher serum osteocalcin concentration (p<0.05). The PTH-AA group in controls had a significantly higher level of serum osteocalcin, this suggests a higher state of bone turnover in the AA genotype.
Conclusion: The outcome of this study proposes the probability of a small impact of the VDR- ApaI (GG) genotype, the VDR-TaqI (TT) genotype and the (AA) genotype of the PTH-BstBI polymorphism indicating a higher rate of bone turnover in the healthy postmenopausal women.
Estimation of the economic value of irrigation water in canal and tube well command areas
Authors: Upadhyaya Ashutosh , Jeet Pawan , Singh Anil Kumar , Sundaram Prem Kumar 
Published: April 2023

AbstractIn recent years, inequality in the distribution of tube wells and non-availability or non-accessibility of canal water during the rabi season leads to a lack of economic value fixation of irrigation water to crops. The main objective of this paper is to calculate the economic value of irrigation water for major crops in the Paliganj distributary of the Sone canal system and the Nalanda corridor site of tube well irrigation, Bihar, India using the Residual Value Method (RVM). Results revealed that the average irrigation water economic value on the basis of applied irrigation through the canal and tube well in the Paliganj distributary for rice, kharif maize, wheat, lentil, khesari, gram, rabi maize, potato, onion, and green gram crops was assessed as 3.73, 22.60, 11.67, 21.50, 27.42, 23.27, 14.75, 98.06, 84.92, and 27.17 Rs./m3, respectively. In the Nalanda corridor, the average irrigation water economic value of rice, kharif maize, wheat, lentil, gram, pea, mustard, potato, rabi maize, and green gram crops was assessed as 12.54, 24.61, 18.71, 44.15, 39.53, 37.85, 32.35, 96.93, 15.44, and 30.25 Rs./m3, respectively.
Draft Genome Sequence of Enterobacter cloacae S23 a Plant Growthpromoting
Passenger Endophytic Bacterium Isolated from Groundnut
Nodule Possesses Stress Tolerance Traits
Authors: Ramakrishnan Pavithra , Ariyan Manikandan , Rangasamy Anandham , Rajasekaran Raghu , Ramasamy Krishnamoorthy , Murugaiyan SenthilKumar , Janahiraman Veeranan 
Published: April 2023

Aim:
This study aims to reveal the passenger endophytic bacterium Enterobacter cloacae
S23 isolated from groundnut nodules and to underpin the molecular mechanism and genes responsible
for abiotic stress tolerance.

Background:
A variety of microorganisms that contribute to nodulation and encourage plant development
activity in addition to the nodulating Rhizobium. Passenger endophytes (PE) are endophytes
that accidentally penetrate the plant without any selective pressure keeping them in the interior tissue
of the plant. PE possesses characteristics that encourage plant development and boost output while reducing
pathogen infection and improving biotic and abiotic stress tolerance. However, there is a lack
of molecular evidence on the passenger endophyte-mediated alleviation of abiotic stresses.

Objective:
This study was formulated to reveal the draft genome sequence of Enterobacter cloacae
S23, as well as genes and characteristics involved in plant growth promotion and stress tolerance.

Method:
The data were submitted to PATRIC and the TORMES-1.0 Unicyclker tools were used to
conduct a complete genome study of Enterobacter cloacae S23. The TORMES-1.0 platform was used
to process the reads. RAST tool kit (RASTtk) was used to annotate the S23 sequence. The plant
growth-promoting traits such as indole acetic acid production, siderophore secretion, production of extracellular
polysaccharides, biofilm formation, phosphate solubilization, and accumulation of osmolytes
were examined under normal, 7% NaCl and 30% polyethylene glycol amended conditions to determine
their ability to withstand salt and moisture stressed conditions, respectively.

Result:
We report the size of Enterobacter cloacae S23 is 4.82Mb which contains 4511 proteincoding
sequences, 71 transfer RNA genes, and 3 ribosomal RNA with a G+C content of DNA is
55.10%. Functional analysis revealed that most of the genes are involved in the metabolism of amino
acids, cofactors, vitamins, stress response, nutrient solubilization (kdp, pho, pst), biofilm formation
(pga) IAA production (trp), siderophore production (luc, fhu, fep, ent, ybd), defense, and virulence.
The result revealed that E. cloacae S23 exhibited multiple plant growth-promoting traits under abiotic
stress conditions.

Conclusion:
Our research suggested that the discovery of anticipated genes and metabolic pathways
might characterise this bacterium as an environmentally friendly bioresource to support groundnut
growth through several mechanisms of action under multi-stresses.
The Effect of Beta-lactoglobulin Nanocapsules Containing Astaxanthin
and 5-fluorouracil on the Antioxidant Enzymes Activity of Superoxide
Dismutase, Catalase and Glutathione Peroxidase in HCT116 Colorectal
Cancer Cell Line
Authors: Gholami Hamid , Abbasalipourkabir Roghayeh , Ziamajidi Nasrin , Divsalar Adeleh , Saeidifar Maryam , Zarei Sadegh 
Published: April 2023

Background:
The use of nanoparticle drug delivery systems to enhance the therapeutic efficacy
and reduce the side effects of anticancer drugs is taken into consideration. Astaxanthin (ATX)
is a natural xanthophyll carotenoid with antioxidant, anti-inflammatory, and antiapoptotic properties
used to prevent and treat some cancers.

Objectives:
In the present study, the antioxidant effect of beta-lactoglobulin (β-LG) nanocapsules containing
ATX and 5-fluorouracil (5-FU; the first-line therapy for colorectal cancer) on the antioxidant
enzymes activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX)
in HCT116 colorectal cancer cell line was examined.

Methods:
In this experimental study, HCT116 cells were treated with different treatments of encapsulation
of ATX in β-LG, encapsulation 5-FU in β-LG, co-encapsulation of ATX and 5-FU in β-LG, free
ATX, free 5-FU, free ATX and free 5-FU, or β-LG nanocapsules without drugs for 24, 48 and 72
hours. There is a control group in which HCT116 cells were not treated with any drug. Then, 50% inhibitory
concentration (IC50) and cell viability were determined using an MTT assay. The antioxidant
enzyme activity of SOD, CAT, and GPX was measured by a colorimetric method in HCT116 cells.

Results:
Different treatments reduced the cell viability and increased apoptotic cells in a timedependent
manner, which was significant for beta-lactoglobulin nanocapsules treatment (P<0.05). It
means receiving more 5-FU or ATX in the encapsulated form by HCT116 cells. The antioxidant enzyme
activity of SOD, CAT, and GPX in HCT116 cells treated with beta-lactoglobulin nanocapsule
treatment significantly increased compared to the control group (P<0.001). Moreover, the antioxidant
activity of these enzymes in different treatments containing ATX (free or encapsulation) was significantly
higher than in other treatments (P<0.05). The most increase in the activity of antioxidant enzymes
is recorded in the treatment of nanocapsules containing ATX and 5-FU simultaneously.

Conclusion:
Increased activity of antioxidant enzymes in addition to the induction of apoptosis in colorectal
cancer cells by various treatments of beta-lactoglobulin nanocapsules indicates more effective
drug administration in encapsulated form as well as synergistic thera[peutic effects of ATX and 5-FU.
Moreover, the main increase in antioxidant enzyme activity may be related to ATX.
ОСОБЛИВОСТІ ПЕРЕБІГУ ПНЕВМОНІЇ, АСОЦІЙОВАНОЇ З КОРОНАВІРУСНОЮ ХВОРОБОЮ COVID-19, У ПАЦІЄНТІВ, ЯКІ ОТРИМУВАЛИ СТАТИНОТЕРАПІЮ
Authors: Н. М. Середюк  , О. З. Скакун  
Published: April 2023

Незважаючи на вакцинацію, а також використання специфічних моноклональних антитіл, коронавірусна хвороба (COVID-19) є важли- вою медико-соціальною проблемою. Коморбідна патологія, зокрема ар- теріальна гіпертензія, можуть обтяжувати перебіг COVID-19. Метою дослідження було вивчити особливості клінічного перебігу COVID-19 у пацієнтів з артеріальною гіпертензією, які отримували статинотера- пію. У дослідження включено 29 пацієнтів, які отримували статини у додатку до базової терапії (основна група), та 77 пацієнтів, які отриму- вали лише базову терапію (контрольна група). Рівень sIL-2R на момент виписки був нижчим у пацієнтів основної групи (3,4 [2,6–4,2] нг/мл), ніж у пацієнтів контрольної групи (4,7 [3,3–6,8] нг/мл) (р=0,03). Статини продемонстрували тенденцію до зменшення кількості пацієнтів, у яких спостерігалося зниження сатурації до рівня <93% за час усього стаці- онарного лікування (відношення шансів – 0,46 [0,19–1,14], р=0,09). Аналіз по підгрупах пацієнтів показав, що статини зменшували ймовірність зни- ження сатурації киснем крові до рівня <93% за весь час стаціонарного лікування серед пацієнтів віком ≥65 років на 72,9% (р=0,03), а також у пацієнтів з ІМТ ≥25,0 кг/м2 на 71,7% (р=0,02). Також статини зменшували ймовірність зниження сатурації киснем крові до рівня <93% за весь час стаціонарного лікування у хворих на цукровий діабет (р=0,07). Встановлено тенденцію до статистичної достовірності щодо зменшення ймовірності розвитку тяжкого/критичного перебігу COVID-19 у пацієнтів основної групи з ІМТ ≥25,0 кг/м2 (р=0,08) та з цукровим діабетом (р=0,098). Таким чином, статини полегшували клінічний перебіг COVID-19, а особливо зна- чний позитивний ефект від застосування статинів спостерігався у паці- єнтів віком ≥65 років, з ІМТ ≥25,0 кг/м2, а також у пацієнтів з цукровим діабетом.
Ключові слова: COVID-19, коронавірусна хвороба, статини, артері- альна гіпертензія.
The Influence of Paid Memberships on Physician Rating Websites With the Example of the German Portal Jameda: Descriptive Cross-sectional Study
Authors: Armbruster Friedrich Aaron David , Brüggmann Dörthe , Groneberg David Alexander , Bendels Michael 
Published: April 2023

Background
The majority of Germans see a deficit in information availability for choosing a physician. An increasing number of people use physician rating websites and decide upon the information provided. In Germany, the most popular physician rating website is Jameda.de, which offers monthly paid membership plans. The platform operator states that paid memberships have no influence on the rating indicators or list placement.

Objective
The goal of this study was to investigate whether a physician’s membership status might be related to his or her quantitative evaluation factors and to possibly quantify these effects.

Methods
Physician profiles were retrieved through the search mask on Jameda.de website. Physicians from 8 disciplines in Germany’s 12 most populous cities were specified as search criteria. Data Analysis and visualization were done with Matlab. Significance testing was conducted using a single factor ANOVA test followed by a multiple comparison test (Tukey Test). For analysis, the profiles were grouped according to member status (nonpaying, Gold, and Platinum) and analyzed according to the target variables—physician rating score, individual patient’s ratings, number of evaluations, recommendation quota, number of colleague recommendations, and profile views.

Results
A total of 21,837 nonpaying profiles, 2904 Gold, and 808 Platinum member profiles were acquired. Statistically significant differences were found between paying (Gold and Platinum) and nonpaying profiles in all parameters we examined. The distribution of patient reviews differed also by membership status. Paying profiles had more ratings, a better overall physician rating, a higher recommendation quota, and more colleague recommendations, and they were visited more frequently than nonpaying physicians’ profiles. Statistically significant differences were found in most evaluation parameters within the paid membership packages in the sample analyzed.

Conclusions
Paid physician profiles could be interpreted to be optimized for decision-making criteria of potential patients. With our data, it is not possible to draw any conclusions of mechanisms that alter physicians’ ratings. Further research is needed to investigate the causes for the observed effects.
Physicochemical Characterization Patin Fish Oil (Pangasius micronema) Is Refined Using Bentonite and Activated Carbon.
Authors: Indah Indah , Rohman Abdul , Lestari Lily Arsanti 
Published: April 2023

Abstract: This study aims to determine the physical and chemical quality of fish oil from the flesh of Pangasius microcinema. The quality of extracted fish oil can be improved through a refining process. Before and after the purification process, the quality of crude fish oil was analyzed, including acid value (AV), saponification value (SV), iodine value (IV), peroxide value (PV), and p-anisidine value (An-V). The results of the characterization of patin fish oil (PFO) in meat were carried out with 3 purifications, namely purification without activated carbon purification treatment with bentonite, the value of susceptibility to acid value, peroxide value, saponification value, iodine value, and p-anisidinee value, which ranged from 1.92-2.64 mg KOH/g, peroxide value 3.76-4.87 meq O2/kg, iodine value 80.46-81.39 g I2/100g, saponification value 189.48-196.75 mg KOH/g, and p-anisidinee value 5.95-8.93 meq/kg. The best refining process is the oil extracted from patin fish oil meat oil and distilled using bentonite from dry rendering because the processed fish oil is good in the standards set by the International Association of Fish Flour Producers, Indonesian Fish Oil Standards, and as food fish oil grades.
HEAVY CADMIUM METAL DETERMINATION CONCENTRATION IN CHEESE
Authors: MUNEAM RUAA R. , ABOJASSIM ALI ABID , JAAFAR KATHEM GHADEER HAKIM 
Published: April 2023

Objective: The purpose of this study was to look into the concentration of the heavy element Cadmium and its health risks in cheese samples available in Iraqi markets.
Methods: 72 random samples of six country groups (15 Iran, 9 Iraq, 9 Egypt, 15 Turkey, 15 Hungary, and 9 Saudi Arabia) available in Iraqi markets were collected and analyzed using an atomic absorption spectrophotometer, Shimadzu model AA7000, USA.
Results: The highest average Cd metal level in the samples was found in Iranian cheese, while the lowest was in Iraqi ones. The descending Cd order of the countries was Turkey>Hungary>Egypt>Iran>Saudi Arabia>Iraq, according to the T-test confirmation. The daily Cd metal intake ( ), Cd target hazard quotient ( ), and Cd carcinogenic risk ( ) values were less than the permissible and risk values.
Conclusion: Eventually, the health risk parameters revealed that there are no pose risks from those cheeses to Iraqi consumers.
OBJETIVOS DE DESENVOLVIMENTO SUSTENTÁVEL (ODS): UM ESTUDO DE CASO DA RAÍZEN S/A E ENGIE BRASIL ENERGIA
Authors: Oliveira Silva Fernanda , Streit Jorge Alfredo Cerqueira 
Published: April 2023

Objetivo: identificar e analisar as estratégias organizacionais adotadas pelas empresas pesquisadas para o cumprimento de metas da Agenda 2030 da ONU.
Método / abordagem: a pesquisa caracteriza-se como qualitativa-descritiva. Os métodos utilizados na pesquisa foram a pesquisa documental, onde foram analisados os relatórios de sustentabilidade das empresas pesquisadas entre os anos de 2020 a 2021. E a revisão bibliográfica realizada através da análise dos artigos publicados voltados para o tema, sustentabilidade corporativa publicados nos últimos 10 anos.
Principais resultados: observa-se que o tempo de atuação das empresas, o segmento e as políticas de gestão influenciam diretamente no engajamento sustentável das organizações. Questões voltadas para competitividade e consolidação da marca também são fatores para as empresas se tornarem cada vez mais sustentáveis. Os indicadores de enfrentamento aos problemas sociais e a mudança de comportamento dos consumidores também são considerados um grande desafio para as empresas se adequarem aos padrões de sustentabilidade. Na análise documental das empresas Raízen e Engie do Brasil, identica-se que as práticas organizacionais adotadas contribuiram de forma significativa para as empresas atenderem aos indicadores de desempenho exigidos pela Agenda 2030 e como consequência se tornarem organizações que buscam atender os padrões de sustentabilidade.
Contribuições metodológicas / sociais / gerenciais: as práticas, bem como, o arcabouço histórico voltado para o tema. Contribuem para que outras organizações entendam a importância de implementação voltada para a sustentabilidade em suas cadeias produtivas e nas ações de cunho social. Os impactos gerados por práticas como estas, tem ligação direta, não só economicamente para as organizações que as promovem, mas para o meio social e ambiental. Nota-se que as regiões atingidas pelas práticas promovidas por essas companhias possuem um avanço significativo, nas questões sociais, ambientais e econômicas.
Originalidade / relevância: o estudo de caso serviu como instrumento de pesquisa acerca das narrativas empíricas apresentadas. Sua relevância no engajamento temático e no entendimento a luz dos objetivos de desenvolvimento sustentável ODS possuem grande notoriedade tanto na esfera social, econômica e ambiental. Empresas sustentáveis atraem para si, menores custos nas cadeias produtivas, consolidação da marca (marketing verde), facilidades para entrada de novos mercados, melhores condições fiscais e tributárias e satisfação dos colaboradores e dos clientes.