bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comparative GC/MS Analysis of Citrus Essential Oils: Unveiling the Potential Benefits of Herb‐Drug Interactions in Preventing Paracetamol‐Induced Hepatotoxicity
Authors: Shalaby Aya S. , Eid Hanaa H. , El‐Shiekh Riham A. , Youssef Fadia S. , Al‐Karmalawy Ahmed A. , Farag Nahla A. , Sleem Amany A. , Morsy Fatma Adly , Ibrahim Khaled M. , Tadros Soad H. 
Published: August 2023

AbstractOur study aimed to test the potential of Citrus oils in protecting against paracetamol (PAR)‐induced hepatotoxicity. The essential oils of Pineapple sweet orange (OO), Murcott mandarin (MO), Red grapefruit (GO), and Oval kumquat (KO) were investigated using gas chromatography coupled with mass spectrometry (GC/MS). Twenty‐seven compounds were identified, with monoterpene hydrocarbons being abundant class. d‐Limonene had the highest percentage (92.98 %, 92.82 %, 89.75 %, and 94.46 % in OO, MO, GO, and KO, respectively). Hierarchical cluster analysis (HCA) and principal components analysis (PCA) revealed that octanal, linalool, germacrene D, and d‐limonene were the principal discriminatory metabolites that segregated the samples into three distinct clusters. In vitro antioxidant capacities were ranged from 1.2–12.27, 1.79–5.91, and 235.05–585.28 μM Trolox eq/mg oil for 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic (ABTS), ferric‐reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC), respectively. In vivo, citrus oils exhibited a significant reduction in alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and nitric oxide (NO). Additionally, there was an increase in glutathione reductase (GSH), and the liver architecture was nearly normal. Molecular docking revealed that d‐limonene exhibited a good inhibitory interaction with cytochrome P450 (CYP450) isoforms 1A2, 3A4, and 2E1, with binding energies of −6.17, −4.51, and −5.61 kcal/mol, respectively.
Engineering an O‐methyltransferase for the Regioselective Biosynthesis of Hesperetin Dihydrochalcone
Authors: Kunzendorf Andreas , Zirpel Bastian , Milke Lars , Ley Jakob P. , Bornscheuer Uwe T. 
Published: August 2023

AbstractDirected evolution of the O‐methyltransferase ZgOMT from Zooshikella ganghwensis focusing on active site residues resulted in highly regioselective biocatalysts (regioisomeric ratios up to 99 : 1) for the preparation of the taste active hesperetin dihydrochalcone and related compounds. These newly constructed enzyme variants provide an attractive synthesis route for para‐methylation of catechol scaffolds, which is challenging to perform with high regioselectivity utilizing wild‐type O‐methyltransferases.
One‐Step Synthesis of Pseudo‐Boehmite by Carbonation in a Microchannel Reactor
Authors: Zhang Xing , Chen Jiayu , Jiang Tao , Fu Jianmin , Wu Xuantao , Wang Hui , Sun Yuhan 
Published: August 2023

AbstractPseudo‐boehmite with a high specific surface area and large pore volume was continuously synthesized in a microchannel reactor using the carbonation method. The effects of the microchannel on the prepared pseudo‐boehmite, strongly present in gas‐liquid mixing efficiency, were studied. In time scale, the crystallinity of pseudo‐boehmite in the microchannel, without the aging process of high‐temperature stirring, reaches the standard of industrial products. Besides, the fluid and mass transfer effect of the gas‐liquid mixing process in the microreactor was simulated under experimental conditions in computational fluid dynamics. The result illustrated the base‐liquid surround by the acid‐gas model in the microreactor, which is significantly different from the batch reactor.
Structure and Flexibility of Copper‐Modified DNA G‐Quadruplexes Investigated by 19F ENDOR Experiments at 34 GHz**
Authors: Schumann Simon L. , Kotnig Simon , Kutin Yury , Drosou Maria , Stratmann Lukas M. , Streltsova Yana , Schnegg Alexander , Pantazis Dimitrios A. , Clever Guido H. , Kasanmascheff Müge 
Published: August 2023

AbstractDNA G‐quadruplexes (GQs) are of great interest due to their involvement in crucial biological processes such as telomerase maintenance and gene expression. Furthermore, they are reported as catalytically active DNAzymes and building blocks in bio‐nanotechnology. GQs exhibit remarkable structural diversity and conformational heterogeneity, necessitating precise and reliable tools to unravel their structure‐function relationships. Here, we present insights into the structure and conformational flexibility of a unimolecular GQ with high spatial resolution via electron‐nuclear double resonance (ENDOR) experiments combined with Cu(II) and fluorine labeling. These findings showcase the successful application of the 19F‐ENDOR methodology at 34 GHz, overcoming the limitations posed by the complexity and scarcity of higher‐frequency spectrometers. Importantly, our approach retains both sensitivity and orientational resolution. This integrated study not only enhances our understanding of GQs but also expands the methodological toolbox for studying other macromolecules.
Factors associated with participation in cardiac rehabilitation in patients with acute myocardial infarction: A systematic review and meta‐analysis
Authors: Wang Lingyu , Liu Jingyu , Fang Haiyan , Wang Xiang 
Published: August 2023

AbstractBackgroundCardiac rehabilitation (CR) is effective in reducing morbidity and mortality in patients with acute myocardial infarction (AMI), but the participation rate is low and its influencing factors vary. Our study aimed to systematically review the literature and investigate the participation rates and influencing factors of CR in patients with AMI.MethodsWe searched 10 databases, including PubMed, Web of Science, Cochrane Library, and so forth. A systematic review and meta‐analysis were conducted on the studies on the factors affecting CR participation in AMI. The Q tests and the I2 tests were used to assess heterogeneity between studies. The combined effect size and odds ratio (OR) and their respective 95% confidence interval (CI) for CR participation rate and its influences are expressed, respectively. Stata 17.0 software was used for statistical analysis.ResultsWe included 14 studies with 114 542 participants. Current evidence indicates a CR participation rate of 34% (95% CI: 21%–46%) in patients with AMI. The pooled OR values and CI of each influencing factor are as follows: over 60 years old (OR = 0.865; 95% CI: 0.772–0.969), male (OR = 1.690; 95% CI: 1.276–2.239), college education or above (OR = 2.526; 95% CI: 1.117–5.711), ST‐segment elevation myocardial infarction (OR = 4.257; 95% CI: 2.004–9.045), decrease in left ventricular ejection fraction (OR = 0.918; 95% CI: 0.868–0.971), higher economic level (OR = 1.282; 95% CI: 1.108–1.483), history of coronary heart disease(OR = 0.667; 95% CI: 0.509–0.875), smoking (OR = 0.665; 95% CI: 0.550–0.805), combined hypertension (OR = 0.638; 95% CI: 0.562–0.723), and combined hyperlipidemia (OR = 0.577; 95% CI: 0.512–0.651).ConclusionsThe overall participation rate of CR in AMI patients is low, and various factors affect the participation rate. Specialist medical staff are needed to further promote CR rehabilitation concepts and scientific knowledge, and take appropriate measures to address the influencing factors to increase CR utilization and improve patient prognosis.
pH‐Controlled Transition from Disaggregation to Reaggregation of Bis‐indolyl(phenyl)methane−β‐CD Conjugate Appended with Multi‐logic Operations
Authors: Paul Provakar , Samanta Saikat , Mondal Bibhas , Saha Swadhin Kumar , Roy Ujjal Kanti , Mallick Arabinda , Majumdar Tapas 
Published: August 2023

AbstractA multifunctional molecular logic system is designed based on the pH‐responsive emission properties of bis‐indolyl(phenyl)methane (BIPM)−β‐CD conjugates in aqueous medium. In our previous studies, BIPM molecules in water were found to form large molecular self‐aggregates that disaggregated in presence of β‐CD molecules through inclusion complexation. In the present study, transition from the disaggregated to reaggregated form of bis‐indolyl(phenyl)methane−β‐CD conjugate was followed through alteration of the local pH from 7 to 13.2. The BIPM molecules are expected to reaggregate at high alkaline medium due to the expulsion of probes from the nano‐cages of β‐CD. The pH dependent emission spectroscopic changes are harvested to design a multi‐functional molecular logic system that can mimic the functions of several fundamental logic gates and an ‘erase‐read‐write‐read’ memory unit. A macro‐molecular security system with three chemical inputs is also presented that can authenticate all‐active‐all‐possible (33) three‐character passwords with their respective unique fluorescence trajectories. Designing of molecular logic system based on the pH‐responsive emission properties of the aggregated and disaggregated forms of an organic probe is rather uncommon and the multifunctional logic, memory and security applications of the present system will carry significant importance in the molecular logic research.
Complementary analysis of proteome‐wide proteomics reveals changes in RNA binding protein‐profiles during prostate cancer progression
Authors: Aikio Erika , Koivukoski Sonja , Kallio Elina , Sadeesh Nithin , Niskanen Einari A. , Latonen Leena 
Published: August 2023

AbstractBackgroundAccumulating evidence indicates importance of RNA regulation in cancer. This includes events such as splicing, translation, and regulation of noncoding RNAs, functions which are governed by RNA binding proteins (RBPs).AimsTo find which RBPs could be relevant for prostate cancer, we performed systematic screening of RBP expression in clinical prostate cancer.Methods and ResultsWe interrogated four proteome‐wide proteomics datasets including tumor samples of primary, castration resistant, and metastatic prostate cancer. We found that, while the majority of RBPs are expressed but not significantly altered during prostate cancer development and progression, expression of several RBPs increases in advanced disease. Interestingly, most of the differentially expressed RBPs are not targets of differential posttranscriptional phosphorylation during disease progression. The RBPs undergoing expression changes have functions in, especially, poly(A)‐RNA binding, nucleocytoplasmic transport, and cellular stress responses, suggesting that these may play a role in formation of castration resistance. Pathway analyzes indicate that increased ribosome production and chromatin‐related functions of RBPs are also linked to castration resistant and metastatic prostate cancers. We selected a group of differentially expressed RBPs and studied their role in cultured prostate cancer cells. With siRNA screens, several of these were indicated in survival (DDX6, EIF4A3, PABPN1), growth (e.g., EIF5A, HNRNPH2, LRRC47, and NVL), and migration (e.g., NOL3 and SLTM) of prostate cancer cells. Our analyzes further show that RRP9, a U3 small nucleolar protein essential for ribosome formation, undergoes changes at protein level during metastasis in prostate cancer.ConclusionIn this work, we recognized significant molecular alterations in RBP profiles during development and evolution of prostate cancer. Our study further indicates several functionally significant RBPs warranting further investigation for their functions and possible targetability in prostate cancer.
Organic Molecule Bifunctionalized Polymeric Carbon Nitride for Enhanced Photocatalytic Hydrogen Peroxide Production
Authors: Ba Guiming , Hu Huilin , Chen Xin , Hu Shan , Ye Jinhua , Wang Defa 
Published: August 2023

AbstractModifying the polymeric carbon nitride (CN) with organic molecules is a promising strategy to enhance the photocatalytic activity. However, most previously reported works show that interchain embedding and edge grafting of the organic molecule can hardly be achieved simultaneously. Herein, we successfully synthesized organic molecule bifunctionalized CN (MBCN) through copolymerization of melon and sulfanilamide at a purposely elevated temperature of 550 °C. In MBCN, the edge grafted and interchain embedded benzene rings act as the electron‐donating group and charge‐transfer channel, respectively, rendering efficient photocatalytic H2O2 production. The optimal MBCN exhibits a significantly improved non‐sacrificial photocatalytic H2O2 generation rate (54.0 μmol g−1 h−1) from pure water, which is 10.4 times that of pristine CN. Experimental and density functional theory (DFT) calculation results reveal that the enhanced H2O2 production activity of MBCN is mainly attributed to the improved photogenerated charge separation/transfer and decreased formation energy barrier (▵G) from O2− to the intermediate 1,4‐endoperoxide (⋅OOH). This work suggests that simultaneous formation of electron donating group and charge transfer channel via organic molecule bifunctionalization is a feasible strategy for boosting the photocatalytic activity of CN.
An optimal linear and elliptical antenna array design using black widow optimisation for 5G communication
Authors: Saha Rajrup , Das Avishek , Mandal Durbadal , Kar Rajib 
Published: August 2023

SummaryThis research illustrates a precise linear and elliptical antenna array design for synthesising the optimal far‐field radiation pattern in the fifth‐generation (5G) communication spectrum using a meta‐heuristic optimisation technique known as black widow optimisation (BWO). 5G communication is an emerging technology with revolutionary changes in the wireless communication system with ultra‐high data rate, enhanced capacity, low latency and good quality of service. An accurate antenna array design for an ideal far‐field radiation pattern synthesis with a suppressed side lobe level (SLL) value and half power beam width (HPBW) is the most crucial aspect of 5G communications. A suppressed SLL is necessary to reduce interference in the entire side lobe region, whereas a low HPBW is required for long‐distance communication. Here, the BWO is employed to find the optimal feeding current to each array element to lower the SLL and the HPBW value. The BWO algorithm sustains impeccable equity between the exploration and exploitation stages to impact different potential regions of the search space and generate new solutions to attain the global optima by evading the trap of local optima. The design examples of the linear antenna array (LAA) and elliptical antenna array (EAA) are illustrated in this article by applying the optimal feeding currents to each array element. Compared to the uniform antenna array and methodologies described in the recently published literature, the results obtained utilising the BWO algorithm for designing the LAAs and EAAs demonstrate a substantial development in the reduction of SLL and HPBW.
Vicarious facilitation of facial responses to pain
Authors: Göller Peter J. , Reicherts Philipp , Lautenbacher Stefan , Kunz Miriam 
Published: August 2023

AbstractIntroductionObserving facial expressions of pain has been shown to lead to increased subjective, neural and autonomic pain responses. Surprisingly, these vicarious facilitation effects on its corresponding response channel, namely facial responses to pain have mostly been neglected. We aim to examine whether the prior exposure to facial expressions of pain leads to a facilitation of facial responses to experimental pain; and whether this facilitation is linked to the valence (pain vs. neutral expression) or also linked to specific motor‐features of the facial pain expressions (different facial muscle movements).MethodSubjective (intensity and unpleasantness ratings) and facial responses (Facial Action Coding System) of 64 participants (34 female) to painful and non‐painful heat stimuli were assessed. Before each heat stimulus, video clips of computer‐generated facial expressions (three different pain expressions and a neutral expression) were presented.ResultsThe prior exposure to facial expressions of pain led to increased subjective and facial responses to pain. Further, vicarious pain facilitation of facial responses was significantly correlated with facilitation of unpleasantness ratings. We also found evidence that this vicarious facilitation of facial responses was not only linked to the presentation of pain versus neutral expressions but also to specific motor‐features of the pain cue (increase in congruent facial muscle movements).DiscussionVicarious pain facilitation was found for subjective and facial responses to pain. The results are discussed with reference to the motivational priming hypothesis as well as with reference to motor priming.SignificanceOur study uncovers evidence that facial pain responses are not only influenced by motivational priming (similar to other types of pain responses), but also by motor‐priming. These findings shed light on the complexity ‐ ranging from social, affective and motor mechanisms ‐ underling vicarious facilitation of pain.
Optimization of rAAV capture step purification using SO3 monolith chromatography
Authors: Bažec Kaja , Krašna Mirjam , Mihevc Andrej , Leskovec Maja , Štrancar Aleš , Tajnik Sbaizero Mojca 
Published: August 2023

AbstractAdeno‐associated virus (AAV) vectors are crucial tools for gene therapy applications. As AAVs are administered in vivo, stringent purity requirements must be met, necessitating the development of various downstream processing strategies in accordance with regulatory guidelines. In this context, we focus on the non‐affinity serotype‐independent recombinant AAV (rAAV) capture step, which involves the use of Convective Interaction Media (CIM) cation‐exchange SO3 monoliths. We analyzed differentially pretreated viral samples obtained from the Sf9 cell line and applied these samples to the capture SO3 chromatography step. We conducted screening experiments using CIM SO3 0.05 mL monolithic 96‐well plates with buffers of varying pH, sodium chloride concentrations, and the inclusion of poloxamer 188, aiming to select the optimal binding mobile phase. Dynamic binding capacity was defined for different pretreatments and the optimal conditions were subsequently retested using the industrial purification CIMmultus line. The results demonstrated a high overall vector recovery (51%) and a significant reduction in impurities (99.98% for protein reduction and 99.25% for DNA reduction) using the selected capture step parameters, thereby confirming the successful optimization of the rAAV capture step in the downstream process using monoliths.
Tandem catalysts CuSe/AuX for increasing local *CO concentration to promote the photocatalytic CO2 reduction to C2H4
Authors: Yan Yifan , Wang Hongzhi , Bi Xinze , Zhao Yuezhu , Wang Wenhang , Wu Mingbo 
Published: August 2023

AbstractIt is highly desired yet challenging to strategically steer CO2 reduction reaction (CO2RR) toward ethylene (C2H4) with high activity under visible light irradiation. The key to achieving this goal is increasing the local *CO concentration on the catalyst surface and promoting the C‐C coupling progress. Here, we prepare tandem catalysts of CuSe/AuX to realize the photocatalytic reduction of CO2 to C2H4 with high activity. Under light irradiation, the loaded Au NPs are used to activate and transfer CO2 to *CO. The generated *CO intermediate could migrate to the surface of CuSe and cause the C‐C coupling process. Moreover, the theoretical calculation results show that the transport process of *CO from Au NPs to CuSe is spontaneous, which plays a critical role in guaranteeing the high concentration of *CO intermediate on the surface of CuSe. This work not only reveals the effect of tandem catalysis on CO2RR to C2 products but also explores the most suitable tandem catalyst to produce C2H4 with high activity by adjusting the loading amounts of Au NPs. Thus, it provides a way to adjust the Cu‐based catalyst used in the production of C2 products by photocatalytic CO2RR, which may attract extensive attention in the field.
Long‐term seizure outcome and mobility after surgical treatment for Rasmussen encephalitis in children: A single‐center experience
Authors: Nava Brenda Cristina , Costa Ursula Thome , Hamad Ana Paula Andrade , Garcia Camila Araujo Bernardino , Sakamoto Americo Ceiki , Aragon Davi Casale , Machado Helio Rubens , Santos Marcelo Volpon 
Published: August 2023

AbstractObjectiveRasmussen Encephalitis (RE) is a rare inflammatory neurodegenerative disease associated with refractory seizures, hemiparesis, and cognitive deterioration, due to lateralized cortical atrophy. Hemispheric surgery (hemispherotomy) is the mainstay of treatment, but its unavoidable motor deficits and lack of long‐term data regarding seizure outcomes can make patients and families apprehensive to undergo this procedure. The present study aimed at analyzing the results of surgical treatment for RE from a motor and epilepsy standpoint, and mitigate such concerns.MethodsClinical and operative data were retrospectively collected from medical records of pharmacoresistant patients treated with functional hemispherectomy at a tertiary reference center for epilepsy surgery, during a 24‐year period (1996–2020). Variables such as age of epilepsy onset, seizure semiology, seizure frequency, immunomodulatory therapy, age at surgery, duration of epilepsy, surgical procedures and complications, number of medications used preoperatively and postoperatively were described and statistically analyzed.ResultsForty‐three (43) patients were included in this study. Mean age of epilepsy onset was 6.14 years, the average interval between epilepsy onset and hemispherotomy was 2.21 years. and the mean age at surgery was 8.28 years. Thirty patients (69.7%) were Engel I at their last follow‐up, of whom 23 (56.4%) were Engel Ia, within a mean follow‐up of 11.3 years. Duration of epilepsy, seizure frequency, and age at surgery, among others, did not correlate with seizure outcome, except the use of immunotherapy which led to worse outcomes (p < .05). Also, after surgery, motor functionality was significantly recovered (i.e., most patients returned to their previous status) with time.SignificanceThis study tackled some issues regarding the surgical treatment of this disease, particularly showing that hemispherotomy is safe and leads to potentially recoverable disability of motor functions while providing high rates of effective and long‐lasting seizure control; therefore, early surgical indication should be warranted once medical refractoriness has been established.