bluuebunny/crossref_metadata_2025_split huggingface dataset:
METTL3/IGF2BP2 Promotes the Malignant Progression of Esophageal Cancer by Activating the PIK3CA/AKT Pathway
Authors: Guo Xinmeng , Huang Anqi , Qi Ya'nan , Chen Jiaqi , Yang Meng , Jin Mulan 
Published: February 2025

ABSTRACTEsophageal cancer (EC) is a leading cause of cancer‐related mortality worldwide. Methyltransferase‐like 3 (METTL3), a key enzyme involved in m6A methylation, has been implicated in the development and progression of various cancers, including EC. However, its potential mechanism of action in EC progression remains unclear. METTL3 expression was found to be upregulated in EC tissues and cells. Knockdown of METTL3 suppressed EC cell proliferation, invasion, migration, and angiogenesis, while promoting apoptosis. Mechanistically, METTL3 maintained PIK3CA mRNA expression and stability in an m6A‐dependent and IGF2BP2‐dependent manner, respectively. METTL3 silencing inactivated the AKT pathway by regulating PIK3CA expression. Furthermore, overexpression of PIK3CA mitigated the effects of METTL3 silencing on the malignant growth of KYSE180 and TE1 cells in vivo and in vitro. METTL3/IGF2BP2 promoted the malignant progression of EC by activating the PIK3CA/AKT pathway. Targeting the METTL3‐PIK3CA axis may offer a novel therapeutic approach for EC treatment.
A new genus and two new species of gall midges (Diptera: Cecidomyiidae) feeding on Guinea grass Megathyrsus maximus (Poaceae) in Africa
Authors: Kolesik Peter , Sutton Guy F. , van Steenderen Clarke J. M. , Martins Dino J. , Plowes Rob , Paterson Iain D. 
Published: February 2025

AbstractTwo new species of gall midges are described whose larvae feed on the small morphotype of Guinea grass Megathyrsus maximus in Africa. Arabukodiplosis basalis Kolesik, gen. et sp. nov. causes galls at the base of plant's crown in Kenya and Arabukodiplosis vesicaria Kolesik, gen. et sp. nov. causes blister galls on the stems in South Africa and Kenya. Description of the morphology and the sequence of a fragment of the COI mitochondrial gene of the insects are provided. A new genus is erected to contain the two new species. Arabukodiplosis Kolesik, gen. nov. belongs to the supertribe Cecidomyiidi and its closest relative is Mitodiplosis Kieffer, 1914, an African genus containing a single species that induces stem thickening galls on pyp grass Ehrharta villosa (Poaceae) in South Africa. Like Mitodiplosis, Arabukodiplosis Kolesik, gen. nov. cannot be satisfactorily accommodated in any of the currently recognised tribes. The crown of the plant, where A. basalis Kolesik, gen. et sp. nov. forms galls, is where new tillers and shoots originate, so the galls develop where stems would usually form. Stems infested by A. vesicaria Kolesik, gen. et sp. nov. continue to develop above the galls, but the gall is expected to act as a resource sink, reducing the fitness of the host plant. Both species are possible candidates for biological control of M. maximus, which is a serious invasive alien pest outside of its native distribution.
Perioperative Mechanical Circulatory Support for Cardiac Assistance in Thoracic Surgery: A Scoping Review
Authors: Agosta Viviana Teresa , D’Andria Ursoleo Jacopo , Bottussi Alice , Bugo Samuele , Monaco Fabrizio 
Published: February 2025

ABSTRACTBackgroundPatients with pre‐existing severe cardiovascular comorbidities are often deemed ineligible for potentially life‐saving thoracic surgeries and are referred to other conservative therapies. However, this patient population may theoretically benefit from the timely perioperative implantation of temporary mechanical circulation support (tMCS) to both mitigate the surgical stress and stabilize hemodynamics. We performed a scoping review to summarize the evidence regarding the use of tMCS in thoracic surgery.MethodsWe conducted a systematic search across PubMed/MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, Web of Science, Scopus, and Google Scholar from their inception, aided by a search string encompassing a combination of terms for the key research concepts: i) tMCS devices; ii) thoracic surgery procedures (except lung transplantation); and iii) the adult population.ResultsFifteen studies pertinent to the research question, which summarized data from 28 patients, were retrieved for inclusion. In 14 patients, tMCS implantation occurred as a “pre‐emptive” strategy, while the remaining patients had tMCS implanted either intra and/or postoperatively as a “bail‐out” strategy. Specifically, 14 patients required an intra‐aortic balloon pump, 10 veno‐arterial extracorporeal membrane oxygenation, two required a multidevice strategy, and one cardiopulmonary bypass. The relative risk analysis revealed that the mortality rate in the pre‐emptive group was half that of the bail‐out group. Additionally, the risk of both infectious and vascular complications was lower in the pre‐emptive group compared to the bail‐out tMCS strategy.ConclusionWe found that the timely implantation of tMCS in thoracic surgery—either to mitigate patients' heightened cardiovascular risk or as a rescue strategy in the event of life‐threatening surgical complications—may lead to better patient outcomes, as well as allowing them to undergo curative surgery with an acceptable safety profile, characterized by overall good survival rates and a low incidence of device‐related complications.
Laparoscopic Sigmoidectomy in a Male Colon Cancer Patient With Pelvic Arteriovenous Malformation Using Preoperative Interventional Radiology: A Case Report
Authors: Inaguma Gaku , Otsuka Koki , Masumori Koji , Hiro Junichiro , Kumamoto Tsutomu , Kamishima Megumu , Kobayashi Yosuke , Chong Yongchol , Omura Yusuke , Taniguchi Hiroko , Tsujimura Kazuki , Chikaishi Yuko , Tsurumachi Ayako , Akamatsu Hokuto , Uyama Ichiro , Suda Koichi 
Published: February 2025

ABSTRACTPelvic arteriovenous malformation (AVM) is a rare vascular condition with diverse clinical manifestations. Treatment‐related decision‐making is difficult for concurrent AVMs and colon cancer. Interventional radiology is effective for colon cancer patients with pelvic AVM. Herein, a 77‐year‐old man presented with fatigue. Computed tomography revealed thickening of the sigmoid colon wall without lymph node swelling or distant metastasis, confirming irregularly dilated pelvic blood vessels. Preoperative transcatheter embolization of the AVM was initially performed. Then, laparoscopic sigmoidectomy was performed without complications following confirmation of AVM shrinkage via computed tomography. The patient was discharged without complications. Thus, preoperative pelvic AVM embolization in patients with sigmoid colon cancer may facilitate safe minimally invasive surgery.
Effects of Moringa oleifera as a Feed Supplement on Performance and Blood Lipid Profiles of Broiler Chicken: A Meta‐Analysis
Authors: Sukria Heri Ahmad , Risyahadi Sazli Tutur , Foni Lydia Rosa , Aditama Ryzal Satria , Retnani Yuli , Kusuma Raihani Indah , Hasanah Qorry Nurul , Daulai Muhammad Sulaiman , Manik B. T. R. Sri Syntia , Wijayanti Indah , Jayanegara Anuraga 
Published: February 2025

ABSTRACTThis study aimed to evaluate the effects of Moringa oleifera (moringa) as a feed supplement on the performance and health status of broiler chicken through a meta‐analysis. The database was compiled from 35 studies comprising 191 experiments. The literatures from Scopus are screened by PRISMA (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses) protocol, and the data were analyzed using OpenMEE software. Feed containing moringa was the fixed effects and the different studies as random effects. The results showed that dietary moringa supplementation increased weight gain (p < 0.05), decreased feed intake (p < 0.05), and therefore decreased feed conversion ratio (FCR) of the broiler chicken. Blood lipid parameters were also affected by moringa supplementation. Feed containing moringa decreased cholesterol, triglyceride, and LDL (p < 0.05), while HDL was higher in comparison to that of control (p < 0.05). It can be concluded from the meta‐analysis that dietary moringa supplementation effectively improves performance and health status of broiler.
Effect of Telerehabilitation on Handwriting Performance in Children With Attention Deficit Hyperactivity Disorder: Randomized Controlled Trial
Authors: Kaplan Kılıç Büşra , Bumin Gonca , Öğütlü Hakan 
Published: February 2025

ABSTRACTBackgroundChildren with attention deficit hyperactivity disorder (ADHD) frequently encounter challenges in their fine motor skills, an area that therapists target for enhancement through therapeutic interventions. This study aimed to effect of telerehabilitation on handwriting performance in children with ADHD.MethodsIn this randomized controlled trial, 70 children (33 girls and 37 boys) with ADHD aged between 6 and 8 years were randomly assigned to intervention and control groups. Fine motor skills and handwriting performance of both groups were evaluated pre–post‐intervention using the Bruininks‐Oseretsky Test of Motor Proficiency‐2nd Edition (BOT‐2) and Minnesota Handwriting Assessment (MHA) tests. The intervention group received fine motor skills training through telerehabilitation for 8 weeks, 3 days a week, 40 min each session (totalling 24 sessions). The control group was placed on a waiting list for 8 weeks and did not receive any intervention. Statistical methods analysed changes within and between groups pre–post‐intervention.ResultsIn the intervention group, intra‐group change pre–post‐intervention was observed in all subdomains of MHA except legibility (p < 0.05). Compared with the control group, the intervention group showed significant differences in BOT‐2 and MHA's subdomains.DiscussionThe results of this study suggest that fine motor skills training through telerehabilitation may be effective in improving the handwriting performance of children with ADHD.
Clinical Presentation and Nutrition Management of Non‐IgE‐Mediated Food Allergy in Children
Authors: Groetch Marion , Venter Carina , Meyer Rosan 
Published: February 2025

ABSTRACTNon‐IgE‐mediated food allergy encompasses a wide spectrum of allergic disorders ranging from mild to severe presentations, including food protein‐induced allergic proctocolitis, food protein‐induced enterocolitis syndrome, and food protein‐induced enteropathy. Other gastrointestinal symptoms such as constipation and gastroesophageal reflux disease have not previously been recognised as symptoms of non‐IgE‐mediated food allergy in food allergy guideline publications. Recently, two new consensus documents from the European Academy of Allergy, Asthma and Immunology address a potential role of food allergens in these disorders, where standard treatment has failed, and provide guidance for diagnosis and management of these conditions. Additional updates include the World Allergy Organisation guidance on the diagnosis and management of cow's milk allergy, the most commonly implicated food in non‐IgE‐mediated food allergy. These documents all help practitioners to distinguish between common manifestations in infancy that are not pathologic and non‐IgE‐mediated food allergy. Understanding diagnostic criteria is vital to prevent overdiagnosis, limit unnecessary elimination diets and preserve breastfeeding. Conversely, proper diagnosis may reduce symptoms in infants who are affected. Therefore, the first step in management of non‐IgE‐mediated food allergy is establishing a correct diagnosis, which requires understanding the clinical presentations and if needed, applying an appropriate short‐term diagnostic elimination diet to observe for symptom resolution, followed by food reintroduction to assess for symptom recurrence. Once diagnosed, treatment requires removal of the trigger food, most commonly cow's milk, which is not without burden and nutrition risk. Non‐IgE‐mediated food allergies typically resolve early in childhood with some disorders resolving in infancy therefore early reassessment for tolerance is vital. Management of non‐IgE‐mediated food allergy entails following diagnostic algorithms, developing an individualised dietary plan, and timely assessment of tolerance development to reduce burden and nutrition risk.
A Randomized Controlled Trial on the Timing of Soft Tissue Augmentation in Immediate Implant Placement: Soft Tissue Changes and Esthetic Outcome
Authors: Struys Thibault , Christiaens Véronique , De Bruyckere Thomas , Pitman Jeremy , Van Hove Pieter‐Jan , Cosyn Jan 
Published: February 2025

ABSTRACTAimTo assess the impact of the timing of soft tissue augmentation on soft tissue changes and esthetic outcome following single immediate implant placement (IIP) in the anterior maxilla.Materials and MethodsPatients with a failing tooth and intact buccal bone wall in the anterior maxilla (15–25) were enrolled in this RCT. Following single IIP and socket grafting, they were randomly allocated to the control group (immediate soft tissue augmentation performed in the same surgical procedure—ISTA) or the test group (delayed soft tissue augmentation performed 3 months later—DSTA). Implants were placed with a surgical guide and immediately restored with an implant‐supported provisional crown. Changes in soft tissue dimensions were assessed by a blinded clinician using superimposed STL files taken prior to surgery and at 1‐year follow‐up. The esthetic outcome was rated by a blinded clinician and the patient at 1‐year follow‐up. The study adhered to the STA‐COSM (Soft Tissue Augmentation—Core Outcome Set and Measurements for implant dentistry trials) guidelines.ResultsTwenty patients were randomized to each group (control: 16 females, 4 males, mean age 57.6; test: 9 females, 11 males, mean age 54.2). The mean change in buccal soft tissue profile was −0.251 and 0.083 mm for ISTA and DSTA, respectively. The mean difference of −0.318 mm in favor of DSTA was not significant (95% CI: −0.860 to 0.224, p = 0.241). Median midfacial recession was −0.200 and − 0.350 mm for ISTA and DSTA, respectively. The median difference of 0.200 mm in favor of ISTA was not significant (95% CI: −0.210 to 0.700, p = 0.311). Mesial and distal papillary recession, Pink Esthetic Score and patients' esthetic satisfaction failed to demonstrate a significant difference between the groups.ConclusionIn patients with an intact buccal bone wall, the timing of soft tissue augmentation following IIP had no significant impact on soft tissue changes and esthetic outcome.Trial Registration: ClinicalTrials.gov: NCT05537545
SIRT1 Alleviates Oxidative Stress‐Induced Mitochondrial Dysfunction and Mitochondria‐Associated Membrane Dysregulation in Stress Urinary Incontinence
Authors: Chen Liying , Tang Jianming , Zuo Xiaohu , Li Bingshu , Liu Cheng , Hong Shasha , Min Jie , Hu Ming , Li Suting , Zhou Min , Chen Mao , He Yong , Xiao Ya , Huang Xiaoyu , Hong Li 
Published: February 2025

ABSTRACTThe pathogenesis of stress urinary incontinence (SUI), a condition common in women, remains to be fully elucidated. This study revealed that the incidence of SUI is associated with mitochondrial homeostasis dysregulation following oxidative stress in the fibrous connective tissue of the pelvic floor. SIRT1 is an essential factor for maintaining mitochondrial homeostasis; however, its potential role and mechanism of action in SUI pathogenesis remain unclear. Both in vitro and in vivo, we observed that oxidative stress reduced SIRT1 expression to inhibit the PGC‐1α/NRF1/TFAM and PINK1/Parkin signalling pathways, eliciting impairment of mitochondrial biogenesis and mitophagy in L929 cells and SUI mice. Decreased SIRT1 levels induced endoplasmic reticulum (ER) stress and altered the structure of mitochondria‐associated membranes (MAMs), disrupting ER‐mitochondrial calcium homeostasis and exacerbting ROS accumulation. SIRT1 activation can restore mitochondrial function and the structure of MAMs and alleviate ER stress in fibroblasts, promoting anterior vaginal wall repair and improving urodynamic parameters in the SUI model. Our findings provide novel insights into the role and associated mechanism of SIRT1 in ameliorating oxidative stress‐induced mitochondrial dysfunction in fibroblasts of the anterior vaginal wall and propose SIRT1 as a potential therapeutic target for SUI.
Modeling the Interplay Between Viral and Immune Dynamics in HIV: A Review and Mrgsolve Implementation and Exploration
Authors: Vegas Rodriguez Alberto , Velez de Mendizábal Nieves , Girish Sandhya , Trocóniz Iñaki F. , Feigelman Justin S. 
Published: February 2025

ABSTRACTSince its initial discovery, HIV has infected more than 70 million individuals globally, leading to the deaths of 35 million. At present, the annual number of deaths has significantly decreased due to 75% of HIV‐positive individuals being on antiretroviral therapy. Although there is no cure yet, available treatments extend life expectancy, enhance quality of life, and reduce transmission by maintaining viral load below the detection limit of 50 copies/mL, making the individual's levels undetectable and untransmittable. HIV has attracted considerable attention in the computational modeling area, with various models having been developed with different degrees of complexity in an attempt to explain the viral dynamics of the disease. It is important to note that no single model can fully incorporate and predict all the critical factors influencing the dynamics of the disease and its response to treatments. Since the number of published models is large, the purpose of this article is to review several relevant models found in the literature that describe biologically plausible scenarios of HIV infection, including key features of disease progression with or without treatment. A total of 15 models are described, with some implemented in the mrgsolve package in R Studio and shared for the benefit of the scientific community. The modeling framework concerning HIV infection aids in identifying the most impactful parameters within the system and their implications in the model outcomes. Insights provided by these models may help in confirming targets for current and novel therapies, thereby contributing to the exploration of new strategies.
Spitz Spindle Cell/Reed Nevus With SQSTM1::NTRK2 Fusion and Atypical Features in an Older Male Patient: A Case Report and Review of Literature
Authors: Scarfò Federico , Brunetto Emanuela , Magliacane Gilda , Pecciarini Lorenza , Ferrara Gerardo , Rizzo Nathalie 
Published: February 2025

ABSTRACTSpitz lesions display a set of genetic alterations that differ from classical melanocytic lesions: examples include mutations in HRAS and fusions involving ALK, ROS1, MET, MAP3K8, BRAF, and the NTRK genes. We present a Spitz spindle cell/Reed nevus with atypical junctional features and an NTRK2 translocation in a patient of unusual age. The patient was a 61‐year‐old man with a pigmented brown flat 6 mm lesion growing on the skin over the left scapula. The lesion was composed of spindled and epithelioid melanocytes and was arranged in nests with some scattered focal pagetoid cells as well as intraepidermal nests at the center of the lesion and occasional mitotic figures. The melanocytes showed diffuse staining for pan‐Trk antibodies. p16 staining was focally and weakly positive. The cells showed staining for HMB‐45, MART‐1, and tyrosinase, whereas they were negative for PRAME, ALK‐1, and ROS‐1 immunostaining. BAP‐1 was preserved. Next‐generation sequencing detected a SQSTM1::NTRK2 fusion and showed no alterations of ALK, ROS1, RET, NTRK1, and NTRK3 genes, as well as no pathogenic variants of BRAF. Fluorescent in situ hybridization showed NTRK2 translocation in all melanocytes evaluated. This case presents a Spitz nevus with a rare translocation in an older patient.
Emotional Leadership and Teachers' Psychological Well‐Being: The Mediating Role of Emotional Labour and Moderating Roles of Cultural Values in JD‐R Theory
Authors: Zhu Xiaoshuang , Tian Guoxiu , Liu Rui , Li Yanjun 
Published: February 2025

ABSTRACTUsing the job demands‐resources (JD‐R) theory, this study aimed to understand how and when emotional leadership affected teachers' psychological well‐being. It explored the mediating effect of emotional labour and the moderating effects of collectivism and two Confucian cultural values (i.e., harmony and obedience to authority). The sample included 2361 Chinese teachers from primary and secondary schools. Findings showed that emotional leadership as a job resource from leaders promoted deep acting (DA) and expression of naturally felt emotions (ENFE), eventually enhancing psychological well‐being. Moreover, emotional leadership caused more surface acting (SA) only when teachers had a lower level of harmony, which reduced psychological well‐being; collectivism, harmony, and obedience to authority weakened the positive impacts of emotional leadership on psychological well‐being. This study contributes to the JD‐R theory by revealing that leader factors could act as both resources and demands to influence personal factors under the moderating effect of cultural values.
Towards a More Nuanced Understanding of Long‐Distance Rafting: Case Studies From the Southern Ocean
Authors: Spencer Hamish G. , Fraser Ceridwen I. , Poulin Elie , González‐Wevar Claudio A. 
Published: February 2025

ABSTRACTProblemRafting is now recognised as a key process influencing the distribution and connectivity of several marine and coastal species. Rafting scenarios are, however, often invoked in ways that do not account for clear differences in biogeographic outcomes. Here, we illustrate the value of utilising multiple lines of evidence (e.g. different life histories, ecologies, and dispersal frequencies) in elucidating the historical, modern, and future significance of ocean rafting. We discuss these issues using a range of exemplar studies from the Southern Ocean, where rafting clearly underlies many different distributional patterns, although our conclusions hold generally.ExplanationsSuch differences appear to be underpinned by variations in several life‐history characters, with, for instance, direct‐developing taxa more suited to long‐distance rafting events that might span multiple generations. Rafting success is also shaped by a diverse suite of factors including the durability of the raft, the presence/absence of resources and competitors (intra‐ or inter‐specific) at the destination, species' environmental tolerances, latitudinal movements in the position of oceanographic fronts, and the frequency and intensity of extreme events such as storms. Several of these factors are influenced by climate change, so a detailed understanding of their roles is increasingly important—particularly as many species' distributions are shifting.Future ProspectsThe Southern Hemisphere—which has considerably more ocean than the Northern Hemisphere (81% vs. 61%)—provides biogeographers with a wealth of information on such processes, as well as intriguing examples of the puzzles we still face. Powerful new tools, including high‐resolution genomic analyses, ancient DNA, and environmental, ecological and oceanographic modelling, are providing a more granular picture of biogeographical patterns. These novel methods, together with a broader consideration of the factors affecting rafting success, can pave the way for an improved and properly integrated understanding of the eco‐evolutionary outcomes of long‐distance dispersal via rafting.