bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polygonatum odoratum polysaccharide attenuates lipopolysaccharide‐induced lung injury in mice by regulating gut microbiota
Authors: Liu Jia‐rui , Chen Bo‐xue , Jiang Mei‐ting , Cui Tian‐yi , Lv Bin , Fu Zhi‐fei , Li Xue , Du Yao‐dong , Guo Jin‐he , Zhong Xin‐qin , Zou Ya‐dan , Zhao Xin , Yang Wen‐zhi , Gao Xiu‐mei 
Published: August 2023

AbstractPolygonatum odoratum is appreciated for its edible and medicinal benefits especially for lung protection. However, the contained active components have been understudied, and further research is required to fully exploit its potential application. We aimed to probe into the beneficial effects of Polygonatum odoratum polysaccharide (POP) in lipopolysaccharide‐induced lung inflammatory injury mice. POP treatment could ameliorate the survival rate, pulmonary function, lung pathological lesions, and immune inflammatory response. POP treatment could repair intestinal barrier, and modulate the composition of gut microbiota, especially reducing the abundance of Klebsiella, which were closely associated with the therapeutic effects of POP. Investigation of the underlying anti‐inflammatory mechanism showed that POP suppressed the generation of pro‐inflammatory molecules in lung by inhibiting iNOS+ M1 macrophages. Collectively, POP is a promising multi‐target microecological regulator to prevent and treat the immuno‐inflammation and lung injury by modulating gut microbiota.
Brd activity regulates Müller glia‐dependent retinal regeneration in zebrafish
Authors: Lee Jiwoon , Lee Bum‐Kyu , Gross Jeffrey M. 
Published: August 2023

AbstractThe zebrafish retina possesses tremendous regenerative potential. Müller glia underlie retinal regeneration through their ability to reprogram and generate multipotent neuronal progenitors that re‐differentiate into lost neurons. Many factors required for Müller glia reprogramming and proliferation have been identified; however, we know little about the epigenetic and transcriptional regulation of these genes during regeneration. Here, we determined whether transcriptional regulation by members of the Bromodomain (Brd) family is required for Müller glia‐dependent retinal regeneration. Our data demonstrate that three brd genes were expressed in Müller glia upon injury. brd2a and brd2b were expressed in all Müller glia and brd4 was expressed only in reprogramming Müller glia. Utilizing (+)‐JQ1, a pharmacological inhibitor of Brd function, we demonstrate that transcriptional regulation by Brds plays a critical role in Müller glia reprogramming and regeneration. (+)‐JQ1 treatment prevented cell cycle re‐entry of Müller glia and the generation of neurogenic progenitors. Modulating the (+)‐JQ1 exposure window, we identified the first 48 h post‐injury as the time‐period during which Müller glia reprogramming occurs. (+)‐JQ1 treatments after 48 h post‐injury had no effect on the re‐differentiation of UV cones, indicating that Brd function is required only for Müller glia reprogramming and not subsequent specification/differentiation events. Brd inhibition also prevented the expression of reprogramming genes like ascl1a and lepb in Müller glia, but not effector genes like mmp9, nor did it affect microglial recruitment after injury. These results demonstrate that transcriptional regulation by Brds plays a critical role during Müller glia‐dependent retinal regeneration in zebrafish.
Radiomics analysis of intraoral ultrasound images for prediction of late cervical lymph node metastasis in patients with tongue cancer
Authors: Konishi Masaru , Kakimoto Naoya 
Published: August 2023

AbstractBackgroundWe investigated the predictability of late cervical lymph node metastasis using radiomics analysis of ultrasonographic images of tongue cancer.MethodsWe selected 120 patients with tongue cancer who underwent intraoral ultrasonography, 30 of which had late cervical lymph node metastasis. Radiomics analysis was used to extract and quantify the image features. Bootstrap forest (BF), support vector machine (SVM), and neural tanh boost (NTB) were used as the machine learning models, and receiver operating characteristic curve analysis was conducted to determine diagnostic performance.ResultsThe sensitivity, specificity, accuracy, and AUC in the validation group were, respectively, 0.600, 0.967, 0.875, and 0.923 for the BF model; 0.700, 0.967, 0.900, and 0.950 for the SVM model; and 0.900, 0.967, 0.950, and 0.967 for NTB model.ConclusionsRadiomics analysis and machine learning models using ultrasonographic images of pretreated tongue cancer could predict late cervical lymph node metastasis with high accuracy.
Prognostic value of pretreatment serum fibrinogen in young patients with small cell lung cancer: A cross‐sectional study
Authors: Tian Juncai , Tian Huohuan , Yang Mengzhu , Yang Linhui , Liu Dan 
Published: August 2023

AbstractObjectiveTo explored the prognostic value of pretreatment serum fibrinogen level in young patients with small cell lung cancer (SCLC).MethodsThe concentrations of serum fibrinogen of 183 young (<50 years old) SCLC patients were measured. The association of baseline serum fibrinogen level and clinical outcome was analyzed by survival analysis.ResultsHyperfibrinogenemia was significantly associated with American Veterans Administration Lung Study Group stage and surgery treatment. The survival analysis indicated that patients with hypefibrinogenemia had worse outcome than patients with normal fibrinogen. Pretreatment serum fibrinogen level was identified as a worse independent survival predictor in young SCLC. The hazard ratio was 1.420 (95% confidence interval: 1.035−1.947).ConclusionsPretreatment serum fibrinogen is independent associated with overall survival in patients with young SCLC.
Study of weakly nonlinear double‐diffusive magnetoconvection under concentration modulation
Authors: Jakhar Atul , Kumar Anand , Gupta Vinod Kumar 
Published: August 2023

AbstractThis article explains the heat and mass transfer of electrically conducting Newtonian fluid in double‐diffusive magnetoconvective flow. We have considered two infinite horizontal plates at a constant distance apart under the concentration‐modulated boundary condition. A constant magnetic field is considered in vertically upward directions, which generates an induced magnetic field. We have used the weakly nonlinear analysis to obtain the heat and mass transfer rate using the Ginzburg–Landau equation. The software MATHEMATICA is used to determine the solution of the Ginzburg–Landau equation by inbuilt function. The effects of physical parameters that occurred in the study on the Nusselt number and Sherwood number have been examined graphically. Modulation has a negligible effect on the threshold value of the thermal Rayleigh number, that is, on stationary convection. Moreover, it was found that the Chandrasekhar number, magnetic‐Prandtl number, amplitude of modulation, and frequency of modulation are proportional to the heat and mass transports.
Mechanism of Robo1 in the pentylenetetrazol‐kindled epilepsy mouse model
Authors: Liu Zheng , Huang Wei , Zhu Man‐Min , Xu Zhong‐Xiang , Xu Zu‐Cai , Yu Chang‐Yin , Huang Hao 
Published: August 2023

AbstractThe neural network hypothesis is one of the important pathogenesis of drug‐resistant epilepsy. Axons guide molecules through synaptic remodeling and brain tissue remodeling, which may result in the formation of abnormal neural networks. Therefore, axon guidance plays a crucial role in disease progression. However, although Robo1 is one of the important components of axon guidance, the role of Robo1 in epilepsy remains unclear. In this study, we aimed to explore the mechanism of Robo1 in epilepsy. Male adult C57BL/6 mice were intraperitoneally injected with pentylenetetrazol to establish an epilepsy model. Lentivirus (LV) was given via intracranial injection 2 weeks before pentylenetetrazol injection. Different expressions of Robo1 between the control group, LV‐mediated Robo1 short hairpin RNA group, empty vector control LV group, and normal saline group were analyzed using Western blot, immunofluorescence staining, Golgi staining, and video monitoring. Robo1 was increased in the hippocampus in the pentylenetetrazol‐induced epilepsy mouse model; lentiviral Robo1 knockdown prolonged the latency of seizure and reduced the seizure grade in mice and resulted in a decrease in dendritic spine density, while the number of mature dendritic spines was maintained. We speculate that Robo1 has been implicated in the development and progression of epilepsy through its effects on dendritic spine morphology and density. Epileptic mice with Robo1 knockdown virus intervention had lower seizure grade and longer latency. Follow‐up findings suggest that Robo1 may modulate seizures by affecting dendritic spine density and morphology. Downregulation of Robo1 may negatively regulate epileptogenesis by decreasing the density of dendritic spines and maintaining a greater number of mature dendritic spines.
Bladder stone formation around polyethylene glycol after use of SpaceOAR Hydrogel
Authors: Tanaka Gen , Tsuboi Ichiro , Mitani Kazutaka , Yoshioka Saori , Yokoyama Shuhei , Kobayashi Yusuke , Nakajima Hirochika , Nagami Taichi , Ogawa Kohei , Wada Koichiro 
Published: August 2023

IntroductionRadiation therapy is used as primary, adjuvant, and salvage therapy for prostate cancer. When using radiation therapy, the SpaceOAR® system is considered easy to use and useful for reducing the irradiated dose and toxicity to the rectum. Although SpaceOAR® system have been reported some adverse event including death.Case presentationA 74‐year‐old male was diagnosed with prostate cancer of clinical stage cT2aN0M0 and intermediate risk by the National Comprehensive Cancer Network guidelines. We inserted the SpaceOAR® Hydrogel before performing intensity‐modulated radiation therapy, as the patient had ulcerative colitis. We did not recognize any complications during or after the procedure, although magnetic resonance imaging revealed hydrogel in the bladder retrospectively. Fourteen months after the procedure, the patient was presented with macrohematuria and we found a bladder stone including hydrogel.ConclusionWe report the first case of a bladder stone after use of SpaceOAR® Hydrogel. We must be careful of taking place it.
Asymptomatic renal granuloma diagnosed 3 years after Bacillus Calmette–Guérin intravesical injection: A case report and a literature review
Authors: Tsuru Ibuki , Nakamura Masaki , Izumi Taro , Ono Akihiro , Masuda Yoshio , Kusakabe Masashi , Morikawa Teppei , Kume Haruki , Shiga Yoshiyuki 
Published: August 2023

IntroductionIntravesical Bacillus Calmette–Guérin immunotherapy is an effective treatment for non‐muscle‐invasive bladder cancer, which is occasionally associated with side effects and complications. The incidence of significant renal complications after intravesical Bacillus Calmette–Guérin immunotherapy is less than 2%. We report a case of renal granuloma after intravesical Bacillus Calmette–Guérin immunotherapy for bladder cancer, which radiologically resembled a papillary renal cell carcinoma.Case presentationA 65‐year‐old man, who had a medical history of urothelial carcinoma and received intravesical Bacillus Calmette–Guérin therapy, was referred to our Urology Department with a right renal tumor. Imaging findings suggested papillary renal cell carcinoma. Robot‐assisted partial nephrectomy was performed, and the histopathological examination revealed epithelioid cell granuloma, which were considered to be Bacillus Calmette–Guérin‐related renal granuloma.ConclusionBacillus Calmette–Guérin‐related renal granuloma mimicking papillary renal cell carcinoma have been reported. We should consider the possibility of renal granulomas when encountering image abnormalities for patients treated with intravesical Bacillus Calmette–Guérin therapy.
Effect of irrigation with plasma‐activated wastewater on radish growth
Authors: Belov Alexander 
Published: August 2023

AbstractCold atmospheric plasma technology activates water, but the effect of plasma‐activated wastewater on vegetables remains unexplored. The first garden bed was irrigated with wastewater from the Zhulebinsky collector after ultraviolet treatment, and the second bed was irrigated after plasma activation. The third garden bed was irrigated with plasma‐activated wastewater from the Kuryanovsky channel. The bacterial concentration in the water for all experiments was 106 CFU mL−1, the nitrate content in the water for the first garden bed was 6.3 mg L−1, and for the second and third garden beds, it was 10 mg L−1. The average fresh weight of radish roots from the garden bed when irrigated with plasma‐activated water from the Zhulebinsky collector was 7% higher than that when irrigated with ultraviolet‐treated water. The increase in the average fresh weight of stems with leaves was 13%. The highest total and average fresh weights of radish were observed in the garden bed when irrigated with plasma‐activated water from the Kuryanovsky channel. Compared with water irrigation from the Zhulebinsky collector, the increase in average fresh weight was 16%, and compared with irrigation with ultraviolet‐treated water, it was 29%. Reuse of plasma‐activated wastewater increases the yield of vegetables during irrigation and contributes to the saving of fresh water resources.
The longitudinal relationship between social support and victimization among latino teens
Authors: Sabina Chiara , Cardenas Iris , Vásquez Desi , Mariscal Susana , Cuevas Carlos A. 
Published: August 2023

AbstractPurposeThis study examines the relationship between social support and victimization of Latino youth over time, utilizing the stress prevention and support deterioration models.MethodsTo address the research questions we utilized data from Waves 1 and 2 (n = 574) of the Dating Violence among Latino Adolescents (DAVILA) study, a national bilingual phone survey of self‐identified Latino youth and their caregiver. Cross‐lagged panel modeling was used to assess the fit of the two theoretical models to observed patterns of covariance among the victimization and social support variables specified.ResultsResults show that victimization at Wave 1 was positively and strongly related to victimization at Wave 2 and social support at Wave 1 was positively and moderately associated with social support at Wave 2. As hypothesized, higher levels of victimization at Wave 1 were significantly related to decreases in social support at Wave 2 (β = −.15). Wave 1 social support was not significantly related to victimization at Wave 2.ConclusionsWe did not find support for the stress prevention model but did find support for the support deterioration model. Teens who were victimized tended to have lower levels of subsequent social support, highlighting the need to equip peers, family, and significant others to adequately respond to victimization disclosures.
Rapid label‐free detection of early‐stage lung adenocarcinoma and tumor boundary via multiphoton microscopy
Authors: Xi Gangqin , Huang Chen , Lin Jie , Luo Tianyi , Kang Bingzi , Xu Mingyu , Xu Huizhen , Li Xiaolu , Chen Jianxin , Qiu Lida , Zhuo Shuangmu 
Published: August 2023

AbstractLung cancer is the most commonly diagnosed cancer and the leading cause of cancer‐related deaths in China. Rapid and precise evaluation of tumor tissue during lung cancer surgery can reduce operative time and improve negative‐margin assessment, thus increasing disease‐free and overall survival rates. This study aimed to explore the potential of label‐free multiphoton microscopy (MPM) for imaging adenocarcinoma tissues, detecting histopathological features, and distinguishing between normal and cancerous lung tissues. We showed that second harmonic generation (SHG) signals exhibit significant specificity for collagen fibers, enabling the quantification of collagen features in lung adenocarcinomas. In addition, we developed a collagen score that could be used to distinguish between normal and tumor areas at the tumor boundary, showing good classification performance. Our findings demonstrate that MPM imaging technology combined with an image‐based collagen feature extraction method can rapidly and accurately detect early‐stage lung adenocarcinoma tissues.
Development of heparinized and hepatocyte growth factor‐coated acellular scaffolds using porcine carotid arteries
Authors: Cheng Jin , Wang Cong , Guo Lianrui , Gu Yongquan 
Published: August 2023

AbstractTissue‐engineered blood vessel substitutes have been developed due to the lack of suitable small‐diameter vascular grafts. Xenogeneic extracellular matrix (ECM) scaffolds have the potential to provide an ideal source for off‐the‐shelf vascular grafts. In this study, porcine carotid arteries were used to develop ECM scaffolds by decellularization and coating with heparin and hepatocyte growth factor (HGF). After decellularization, cellular and nucleic materials were successfully removed with preservation of the main compositions (collagen, elastin, and basement membrane) of the native ECM. The ultimate tensile strength, suture strength, and burst pressure were significantly increased after cross‐linking. Pore size distribution analysis revealed a porous structure within ECM scaffolds with a high distribution of pores larger than 10 μm. Heparinized scaffolds exhibited sustained release of heparin in vitro and showed potent anticoagulant activity by prolonging activated partial thromboplastin time. The scaffolds showed an enhanced HGF binding capacity as well as a constant release of HGF as a result of heparin modification. When implanted subcutaneously in rats, the modified scaffolds revealed good biocompatibility with enzyme degradation resistance, mitigated immune response, and anti‐calcification. In conclusion, heparinized and HGF‐coated acellular porcine carotid arteries may be a promising biological scaffold for tissue‐engineered vascular grafts.
microRNA‐92b‐3p augments colon cancer development through inhibiting KLF3
Authors: Liu Xuezhong , Zhang Lei 
Published: August 2023

AbstractColon cancer (CC) is a tumor of the large intestine. miR‐92b‐3p is often deregulated in the tumorigensis. Here, the role of miR‐92b‐3p in the development of CC was investigated. miR‐92b‐3p and Kruppel‐like factor 3 (KLF3) expression was examined in CC tissues and cells. miR‐92b‐3p inhibitor or KLF3 overexpression vector was transfected into CC cells, respectively to observe its role in CC cell proliferation, invasion, migration, and apoptosis. The targeting relationship between miR‐92b‐3p and KLF3 was validated. Meanwhile, rescue experiments were performed by co‐transfection of miR‐92b‐3p inhibitor and KLF3 siRNA, followed by determining CC cell proliferation, invasion, migration, and apoptosis. Higher miR‐92b‐3p and lower KLF3 expression levels were observed in CC tissues and cells. miR‐92b‐3p inhibition or KLF3 overexpression reduced proliferation, invasion, and migration whereas induced apoptosis of CC cells. KLF3 was validated to be the target gene of miR‐92b‐3p. Depletion of KLF3 could reverse the antitumor role of miR‐92b‐3p inhibition in CC cells. miR‐92b‐3p augments CC development through inhibiting KLF3, which may confers a novel way to develop future treatment target.
MYH16 upregulation is associated with lung adenocarcinoma aggressiveness and immune infiltration
Authors: Zhang Libin , Liu Jun , Wang Han , Xu Zheyuan , Wang Yang , Chen Yun , Peng Hao 
Published: August 2023

AbstractMyosin heavy chain 16 (MYH16) may significantly affect cell cycle progression. Nevertheless, there is a lack of evidence about the clinical relevance of MYH16 upregulation in pan cancers, including lung adenocarcinoma (LUAD). MYH16 expression patterns were evaluated in various bioinformatics databases using The Cancer Genome Atlas data set. Clinical and pathological factor data were employed to risk‐stratify patients. The Kaplan–Meier plotter approach was used to estimate survival rates. Tumor immune infiltration was explored via the TIMER tool, and gene set enrichment analysis (GSEA) was used to identify the pathways involved in MYH16 upregulation. The results showed that MYH16 was abnormally upregulated in pan cancers, including LUAD. MYH16 expression induction in LUAD was found to be related to the tumor stage. Furthermore, MYH16 upregulation was correlated with LUAD development and worse overall survival, particularly in women. Notably, MYH16 overexpression in LUAD tissues corresponded to the amount of immune infiltration in the tumor. Additionally, univariate Cox hazard regression analysis revealed that MYH16 may be an independent prognostic indicator for LUAD. Furthermore, a nomogram was constructed according to MYH16 expression and clinical characteristics. BMP6 expression deficiency may be a key factor contributing to MYH16 upregulation in LUAD. Finally, GSEA demonstrated that MYH16 might mediate meiosis and gene silencing through RNA signaling pathways. This study, for the first time, showed that MYH16 upregulation in LUAD is associated with various risk factors, increased cancer aggressiveness, enhanced infiltration of tumor immune cells, and reduced survival rates.
Immunomodulatory effect of glabridin in ovalbumin induced allergic asthma and its comparison with methylprednisolone in a preclinical rodent model
Authors: Muhammad Hafsa , Salahuddin Zari , Akhtar Tasleem , Aftab Usman , Rafi Ali , Hussain Safdar , Shahzad Muhammad 
Published: August 2023

AbstractGlabridin, a polyphenolic flavonoid derived fromGlycyrrhiza glabra(licorice) roots, has shown anti‐inflammatory and antioxidant properties. The current study sought to investigate glabridin's immunomodulatory effect in ovalbumin induced allergic asthma. Healthy male Wistar rats were divided into five groups. Group I served as a control group. Asthma was induced in groups II– IV. Groups III and IV were treated with glabridin (40 mg/kg) and methylprednisolone (15 mg/kg), respectively. Inflammatory cells counts were determined in blood and bronchoalveolar lavage fluid (BALF). Serum IgE levels and levels of catalase, superoxide dismutase and glutathione peroxidase in lung homogenate were measured. The levels of mRNA expression of pro‐inflammatory, anti‐inflammatory and oxidative stress markers were analysed. Delayed type hypersensitivity (DTH) and acute toxicity of glabridin were also checked. Glabridin significantly decreased inflammatory cells in the blood and BALF. It increased the concentration of antioxidant enzymes catalase, superoxide dismutase and glutathione peroxidase. Glabridin markedly decreased serum IgE levels and DTH when compared to asthmatic rats. It significantly alleviated the expression of TNF‐α, IL‐4, IL‐5, CXCL1, iNOS, and NF‐κB. Administering 10 times the therapeutic dose of glabridin did not show any signs of acute toxicity. Findings suggest that glabridin has the potential to ameliorate allergic asthma and its effects are comparable to those of methylprednisolone. The immunomodulatory effect of glabridin might be contributed by the suppression of pro‐inflammatory cytokines, oxidative stress markers, IgE antibodies, and elevation of antioxidant enzymes, suggesting future study and clinical trials to propose it as a candidate to treat allergic asthma.