bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sorbitol induces flower bud formation via the MADS‐box transcription factor EjCAL in loquat
Authors: Xu Hong‐Xia , Meng Dong , Yang Qing , Chen Ting , Qi Meng , Li Xiao‐Ying , Ge Hang , Chen Jun‐Wei 
Published: December 2022

ABSTRACTSorbitol is an important signaling molecule in fruit trees. Here, we observed that sorbitol increased during flower bud differentiation (FBD) in loquat (Eriobotrya japonica Lindl.). Transcriptomic analysis suggested that bud formation was associated with the expression of the MADS‐box transcription factor (TF) family gene, EjCAL. RNA fluorescence in situ hybridization showed that EjCAL was enriched in flower primordia but hardly detected in the shoot apical meristem. Heterologous expression of EjCAL in Nicotiana benthamiana plants resulted in early FBD. Yeast‐one‐hybrid analysis identified the ERF12 TF as a binding partner of the EjCAL promoter. Chromatin immunoprecipitation‐PCR confirmed that EjERF12 binds to the EjCAL promoter, and β‐glucuronidase activity assays indicated that EjERF12 regulates EjCAL expression. Spraying loquat trees with sorbitol promoted flower bud formation and was associated with increased expression of EjERF12 and EjCAL. Furthermore, we identified EjUF3GaT1 as a target gene of EjCAL and its expression was activated by EjCAL. Function characterization via overexpression and RNAi reveals that EjUF3GaT1 is a biosynthetic gene of flavonoid hyperoside. The concentration of the flavonoid hyperoside mirrored that of sorbitol during FBD and exogenous hyperoside treatment also promoted loquat bud formation. We identified a mechanism whereby EjCAL might regulate hyperoside biosynthesis and confirmed the involvement of EjCAL in flower bud formation in planta. Together, these results provide insight into bud formation in loquat and may be used in efforts to increase yield.
A culturally adapted parenting intervention for the Chilean context: Qualitative indicators of participant satisfaction and contextual and cultural relevance
Authors: Parra‐Cardona Rubén , Retamal Viviana Muñoz , Fajuri Paulina Peña , Rioseco Luz Cantizano , Mitchell Raúl Perry , Molina María Luisa Correa , Buenabad Nancy Amador 
Published: December 2022

AbstractParent training (PT) interventions constitute an empirically demonstrated alternative to promote effective parenting practices and prevent child behavioral and mental health problems. However, the dissemination of evidence‐based PT interventions across Latin America remains scarce. This qualitative study had the primary objective of evaluating the level of acceptability of a culturally adapted version of the PT intervention known as GenerationPMTO©, adapted for the Chilean context. According to qualitative reports provided by 24 Chilean caregivers exposed to the culturally adapted parenting intervention, the intervention was perceived by caregivers as useful for their parenting practices, as well as contextually and culturally relevant. Current qualitative findings indicate that the culturally adapted PT intervention holds promise for larger dissemination in the Chilean context.
The role of children's hospices in perinatal palliative care and advance care planning: The results of a national British survey
Authors: Tatterton Michael J. , Fisher Megan J. , Storton Helen , Walker Charlotte 
Published: December 2022

AbstractIntroductionPerinatal palliative care services are increasingly available globally, offering a range of clinical and psychological support services to families during pregnancy, in the neonatal period and following the death of a baby with a life‐limiting or life‐threatening condition. Little is understood about the role of children's hospice care and how it contributes to effective perinatal palliative care.DesignThe study aims to answer the question “what is the role of children's hospices in the provision of perinatal palliative care and advance care planning in the United Kingdom?”MethodsAn electronic survey was sent to all 54 children's hospices in the United Kingdom between May and June 2022.ResultsThirty hospices responded, representing 54% of the sector. All regions of all four counties are represented. Numbers of referrals to hospices for perinatal palliative care have increased significantly over the last 5 years. Hospices provide a range of services for families and babies, usually from the point of diagnosis or recognition of a life‐limiting or life‐threatening condition, underpinned with counseling and emotional support. Hospices worked with a range of professionals and services, most commonly fetal medicine and neonatal services. Advance care plans were an important element of effective perinatal palliative care, strengthening parent–professional and interprofessional relationships.ConclusionChildren's hospice services play an important and growing role in the perinatal care of babies and families following the diagnosis or recognition of a life‐limiting or life‐threatening condition. The family‐centered approach to care, from a broad, biopsychosocial perspective means that hospices make a unique and meaningful contribution to both the clinical and psychological needs of families.Clinical relevanceThe family‐centered approach to care, from a broad, biopsychosocial perspective means that hospices make an important contribution to both the clinical needs of babies, and psychological needs of families antenatally, in the neonatal period and after death.
Hypertension treatment in patients with sleep apnea from the European Sleep Apnea Database (ESADA) cohort – towards precision medicine
Authors: Svedmyr Sven , Hedner Jan , Bonsignore Maria Rosaria , Lombardi Carolina , Parati Gianfranco , Ludka Ondrej , Zou Ding , Grote Ludger ,   the European Sleep Apnea Database (ESADA) study group (full collaborator list in E‐supplement)
Published: December 2022

SummaryWe recruited 5,970 hypertensive patients with obstructive sleep apnea (OSA) on current antihypertensive treatment from the European Sleep Apnea Database (ESADA) cohort. The group was subdivided into those receiving monotherapy (n = 3,594) and those receiving dual combined therapy (n = 2,376). We studied how major OSA confounders like age, gender, and body mass index as well as the degree of sleep apnea modified office systolic and diastolic blood pressure. Beta‐blockers alone or in combination with a diuretic were compared with other antihypertensive drug classes. Monotherapy with beta‐blocker was associated with lower systolic blood pressure, particularly in non‐obese middle‐aged males with hypertension. Conversely, the combination of a beta‐blocker and a diuretic was associated with lower systolic and diastolic blood pressure in hypertensive patients with moderate–severe OSA. Systolic blood pressure was better controlled in female patients using this combined treatment. Our cross‐sectional data suggest that specific clinical characteristics and type of antihypertensive medication influence the degree of blood pressure control in hypertensive OSA patients. Controlled trials are warranted.
Direct and indirect genetic effects of a social supergene
Authors: Arsenault Samuel V. , Riba‐Grognuz Oksana , Shoemaker DeWayne , Hunt Brendan G. , Keller Laurent 
Published: December 2022

AbstractIndirect genetic effects describe phenotypic variation that results from differences in the genotypic composition of social partners. Such effects represent heritable sources of environmental variation in eusocial organisms because individuals are typically reared by their siblings. In the fire antSolenopsis invicta, a social supergene exhibits striking indirect genetic effects on worker regulation of colony queen number, such that the genotypic composition of workers at the supergene determines whether colonies contain a single or multiple queens. We assessed the direct and indirect genetic effects of this supergene on gene expression in brains and abdominal tissues from laboratory‐reared workers and compared these with previously published data from field‐collected prereproductive queens. We found that direct genetic effects caused larger gene expression changes and were more consistent across tissue types and castes than indirect genetic effects. Indirect genetic effects influenced the expression of many loci but were generally restricted to the abdominal tissues. Further, indirect genetic effects were only detected when the genotypic composition of social partners differed throughout the development and adult life of focal workers, and were often only significant with relatively lenient statistical cutoffs. Our study provides insight into direct and indirect genetic effects of a social supergene on gene regulatory dynamics across tissues and castes in a complex society.
SUMOylation regulates pre‐mRNA splicing to overcome DNA damage in fungi
Authors: Jian Yunqing , Chen Xia , Sun Kewei , Liu Zunyong , Cheng Danni , Cao Jie , Liu Jianzhao , Cheng Xiaofei , Wu Liang , Zhang Feng , Luo Yuming , Hahn Matthias , Ma Zhonghua , Yin Yanni 
Published: December 2022

Summary

Pathogenic fungi are subject to DNA damage stress derived from host immune responses during infection. Small ubiquitin‐like modifier (SUMO) modification and precursor (pre)‐mRNA splicing are both involved in DNA damage response (DDR). However, the mechanisms of how SUMOylation and splicing coordinated in DDR remain largely unknown.

Combining with biochemical analysis, RNA‐Seq method, and biological analysis, we report that SUMO pathway participates in DDR and virulence in Fusarium graminearum, a causal agent of Fusarium head blight of cereal crops world‐wide. Interestingly, a key transcription factor FgSR is SUMOylated upon DNA damage stress.

SUMOylation regulates FgSR nuclear‐cytoplasmic partitioning and its phosphorylation by FgMec1, and promotes its interaction with chromatin remodeling complex SWI/SNF for activating the expression of DDR‐related genes. Moreover, the SWI/SNF complex was found to further recruit splicing‐related NineTeen Complex, subsequently modulates pre‐mRNA splicing during DDR.

Our findings reveal a novel function of SUMOylation in DDR by regulating a transcription factor to orchestrate gene expression and pre‐mRNA splicing to overcome DNA damage during the infection of F. graminearum, which advances the understanding of the delicate regulation of DDR by SUMOylation in pathogenic fungi, and extends the knowledge of cooperation of SUMOylation and pre‐mRNA splicing in DDR in eukaryotes.
Above‐ and belowground interplay: Canopy CO2 uptake, carbon and nitrogen allocation and isotope fractionation along the plant‐ectomycorrhiza continuum
Authors: Scartazza Andrea , Sbrana Cristiana , D'Andrea Ettore , Matteucci Giorgio , Rezaie Negar , Lauteri Marco 
Published: December 2022

AbstractIn forests, mycorrhizal fungi regulate carbon (C) and nitrogen (N) dynamics. We evaluated the interplay among ectomycorrhizas (ECM), ecosystem C fluxes, tree productivity, C and N exchange and isotopic fractionation along the soil‐ECM‐plant continuum in a Mediterranean beech forest. From bud break to leaf shedding, we monitored: net ecosystem exchange (NEE, a measure of the net exchange of C between an ecosystem and the atmosphere), leaf area index, stem growth, N concentration, δ13C and δ15N in rhizosphere soil, ectomycorrhizal fine root tips (ERT), ECM‐free fine root portions (NCR) and leaves. Seasonal changes in ERT relative biomass were strictly related to NEE and mimicked those detected in the radial growth. The analysis of δ13C in ERT, leaves and NCR highlighted the impact of canopy photosynthesis on ERT development and an asynchronous seasonal C allocation strategy between ERT and NCR at the root tips level. Concerning N, δ15N of leaves was negatively related to that of ERT and dependent on seasonal 15N differences between ERT and NCR. Our results unravel a synchronous C allocation towards ERT and tree stem driven by the increasing NEE in spring‐early summer. Moreover, they highlighted a phenology‐dependent 15N fractionation during N transfer from ECM to their hosts. This evidence, obtained in mature beech trees under natural conditions, may improve the knowledge of Mediterranean forests functionality.
Dye Degradation and Sulfur Oxidation of Methyl Orange and Thiophenol via Newly Designed Nanocomposite GQDs/NiSe‐NiO Photocatalyst Under Homemade LED Light
Authors: Srivastava Shivangi , Yadav Rajesh K. , Pande Poorn Prakash , Singh Satyam , Chaubey Surabhi , Singh Pooja , Gupta Sarvesh Kumar , Gupta Shivani , Kim Tae Wu , Tiwary Dhanesh 
Published: December 2022

AbstractPhotocatalytic processes triggered by graphene‐based photocatalysts under solar light have sparked interest as a new sort of instrument for solar chemical synthesis. Herein we investigated self‐assembled graphene quantum dots (GQDs)/NiSe‐NiO composite photocatalyst for organic transformation as well as dye degradation. The synthesized GQDs/NiSe‐NiO composite photocatalyst has an excellent suitable band gap, high molar extinction coefficient, low toxicity and chemical/thermal stability. The GQDs/NiSe‐NiO composite photocatalyst emerges as a new standard for sulfur oxidation and dye degradation reactions under homemade LED light with high yield.
Environmental considerations in the European Union's pharmaceuticals legislation: Key instruments and their challenges in addressing global manufacturing supply chains
Authors: Kittery Anita , Miettinen Mirella 
Published: December 2022

AbstractThis article analyses how two key instruments of European Union pharmaceuticals legislation—good manufacturing practices and environmental risk assessment of pharmaceuticals—address known risks posed by the release of pharmaceuticals in the environment to human health and the environment and identifies the challenges that they face in regulating global manufacturing supply chains. The analysis reveals that these instruments fail to address the environmental challenges posed by pharmaceuticals within global manufacturing supply chains. Based on these findings, we propose to revise European pharmaceuticals legislation by expanding the scope and information requirements of both human and veterinary environmental risk assessment and increasing its weight in the risk–benefit analysis for human pharmaceuticals; by including direct environmental requirements for certain prioritized pharmaceuticals; and by integrating environmental considerations and related verification mechanisms into good manufacturing practices.
What drives local communities to engage in climate change mitigation activities? Examining the rural–urban divide
Authors: Zeigermann Ulrike , Kammerer Marlene , Böcher Michael 
Published: December 2022

AbstractThe National Climate Initiative (NKI) in Germany provides a strong incentive for local governments to apply for funding to engage in climate protection activities. Yet, the latest evaluation of the NKI confirms that there is a great disparity in the distribution of funding across regions. Taking this regional fragmentation as a starting point, we ask the following: How can we explain disparity in the amount of funding across counties and cities? We draw on climate policy literature pointing to the role of socioeconomic and political determinants and the settlement structure to answer this question. We study all 400 cities and counties to test hypotheses concerning disparity in the likelihood and amount of climate funding, using data from the German Ministry for the Environment and further regional statistics. Running different multilevel regression models, we show that local climate funding is more likely in cities and more urbanized counties than in rural counties with less population and oftentimes also with less young and educated people. We argue that despite the increasing number of local governments getting active in climate protection in Germany, there is important unused potential for climate change mitigation in rural counties. Therefore, we conclude by proposing a new research agenda focusing on climate change mitigation in rural areas.
Severe COVID‐19 patients show a dysregulation of the NLRP3 inflammasome in circulating neutrophils
Authors: Leal Vinicius N. C. , Andrade Milena M. S. , Teixeira Franciane M. E. , Cambui Raylane A. G. , Roa Mariela E. G. V. , Marra Letícia G. , Yamada Suemy M. , Alberca Ricardo W. , Gozzi‐Silva Sarah C. , Yendo Tatiana M. , Netto Lucas C. , Duarte Alberto J. S. , Sato Maria N. , Pontillo Alessandra 
Published: December 2022

AbstractSARS‐CoV‐2 triggers inflammasome‐dependent release of pro‐inflammatory cytokine IL‐1β and pyroptosis, therefore, contributes to the huge inflammatory response observed in severe COVID‐19 patients. Less is known about the engagement of inflammasome in neutrophils, main players in tissue injury and severe infection. We studied the activation of the inflammasome in neutrophils from severe COVID‐19 patients and assessed its consequence in term of cells contribution to disease pathogenesis. We demonstrated that NLRP3 inflammasome is dramatically activated in neutrophils from severe COVID‐19 patients and that the specific inhibition of NLRP3 reverts neutrophils' activation. Next, the stimulation of severe patients' neutrophils with common NLRP3 stimuli was not able to further activate the inflammasome, possibly due to exhaustion or increased percentage of circulating immature neutrophils. Collectively, our results demonstrate that the NLRP3 inflammasome is hyperactivated in severe COVID‐19 neutrophils and its exhaustion may be responsible for the increased susceptibility to subsequent (and possibly lethal) infections. Our findings thus include a novel piece in the complex puzzle of COVID‐19 pathogenesis.
Mass spectrometry in sports drug testing—Analytical approaches and the athletes' exposome
Authors: Thevis Mario , Görgens Christian , Guddat Sven , Thomas Andreas , Geyer Hans 
Published: December 2022

AbstractTest methods in anti‐doping, most of which rely on the most modern mass spectrometric instrumentation, undergo continuous optimization in order to accommodate growing demands as to comprehensiveness, sensitivity, retrospectivity, cost‐effectiveness, turnaround times, etc. While developing and improving analytical approaches is vital for appropriate sports drug testing programs, the combination of today's excellent analytical potential and the inevitable exposure of humans to complex environmental factors, specifically chemicals and drugs at the lowest levels, has necessitated dedicated research, particularly into the elite athlete's exposome. Being subjected to routine doping controls, athletes frequently undergo blood and/or urine tests for a plethora of drugs, chemicals, corresponding metabolic products, and various biomarkers. Due to the applicable anti‐doping regulations, the presence of prohibited substances in an athlete's organism can constitute an anti‐doping rule violation with severe consequences for the individual's career (in contrast to the general population), and frequently the question of whether the analytical data can assist in differentiating scenarios of ‘doping’ from ‘contamination through inadvertent exposure’ is raised. Hence, investigations into the athlete's exposome and how to distinguish between deliberate drug use and potential exposure scenarios have become a central topic of anti‐doping research, aiming at supporting and consolidating the balance between essential analytical performance characteristics of doping control test methods and the mandate of protecting the clean athlete by exploiting new strategies in sampling and analyzing specimens for sports drug‐testing purposes.
Remittance flows and informal economies in post‐Soviet transition countries
Authors: Kakhkharov Jakhongir , Rohde Nicholas 
Published: December 2022

AbstractThis paper studies the impact of remittance flows on the size of informal economies in post‐Soviet countries. We compile a unique data set from a cluster of transition economies and use panel data econometric models to estimate effect sizes. Identification comes from varying macroeconomic conditions in Russia, which are plausibly exogenous to currency demand in remittance–recipient countries. Our estimates indicate that, across our models, it seems plausible that around 25% of all remittances flow into the informal economies of the region. Since shadow economies facilitate numerous illegal activities (such as organised crime, informal money lenders and money laundering), stronger regulatory and institutional frameworks accompanied with provision of incentives for informal firms to be integrated in the formal economy are likely to curb undesirable economic behaviour.
The COVID‐19 pandemic and trade in agricultural products
Authors: Ridley William , Akhundjanov Sherzod B. , Devadoss Stephen 
Published: December 2022

AbstractIn this study, we develop a structural gravity model to analyse the effects of the ongoing COVID‐19 pandemic on international trade in food and agriculture. Using detailed data on trade flows, we estimate the trade impacts of the pandemic for major sectors in food and agriculture. Supply‐side impacts on trade caused by reductions in labour tend to be largest in labour‐intensive sectors such as meat processing and processed fruit and vegetables. The supply‐side export effects are dwarfed by the demand‐side import effects, as the recessionary impact of the pandemic drives significant decreases in imports, largely in processed goods and labour‐intensive commodities.
The associations between diode laser (810 nm) therapy and chronic wound healing and pain relief: Light into the chronic wound patient's life
Authors: Tang Di , Liu Chunmei , Chen Xia , Lv Xian , Yuan Lili , Xue Dongdong , Song Huapei 
Published: December 2022

AbstractChronic wounds have become one of the major issues in medicine today, the treatments for which include dressing changes, negative pressure wound therapy, hyperbaric oxygen, light irradiation, surgery and so forth. Nevertheless, the application of diode lasers in chronic wounds has rarely been reported. This retrospective cohort study aimed to evaluate the therapeutic effect of diode laser (810 nm) irradiation on chronic wounds. Eighty‐nine patients were enrolled in the study. The control group (41 patients) received traditional dressing change therapy, while the diode laser treatment group (48 patients) were patients received additional treatment with diode laser (810 nm) irradiation for 10 min at each dressing change. Wound healing time was compared between two groups, while the pain relief index was creatively introduced to evaluate the effect of relieving wound pain, which was calculated by the difference in pain scores between the first and last dressing changes divided by the number of treatment days. The wound healing time of the diode laser treatment group was 22.71 ± 8.99 days, which was significantly shorter than that of the control group (37.44 ± 23.42 days). The pain relief index of the diode laser treatment group was 0.081 ± 0.055, which was significantly increased compared with that of the control group (0.057 ± 0.033). Our findings suggest that diode laser irradiation has the potential to promote healing in chronic wounds and relieve wound pain.