bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel PPOX mutations in two variegate porphyria pedigrees
Authors: Lei Shubin , Yang Lu , Zhang Ruixuan , Guan Xin , Zhao Xiuli , Wang Tao 
Published: September 2023

Abstract
Objective
Variegate porphyria (VP) is an autosomal dominant inherited disease caused by mutation of the PPOX gene and impacted activity of the protoporphyrinogen oxidase (PPOX). We report two pedigrees of young women who present VP-related manifestation such as blistering, flushing, scarring, and hyperpigmentation after sunlight exposure. The genetic analysis of PPOX gene was performed.
Methods
Whole exome sequencing was conducted for both probands, followed by poly chain reaction (PCR) to amplify interested region and validate the suspicious mutations. Reverse transcriptive PCR was conducted to explore the impact of intronic mutation on the splicing process.
Results
The genetic analysis revealed two novel mutations: c.82_83delCC:p.Pro28* and c.222 + 2delT in heterozygous state. The first mutation leads to a premature termination of PPOX translation and the second one causes the insertion of intron2 between exon2 and exon3. Both of the probands inherit PPOX mutation from their healthy father.
Conclusions
Our study provides molecular diagnosis for two VP pedigrees and identified two novel PPOX mutations. We propose a hypothesis that the incomplete penetrance of VP in these two cases might be associated with sexuality and hormone level.
Toxocara canis infection in multiple types of animals: ophthalmological and pathological observations
Authors: Zheng Shuxin , Sun Limei , Huang Li , Xie Yue , Ding Xiaoyan 
Published: September 2023

Abstract

Human ocular toxocariasis (OT), caused by pet roundworm Toxocara canis (Nematoda Ascaridoidea), is a worldwide ocular parasitic infection severe threat to eyesight, especially in school-aged children. However, the infection process and pathological mechanism of Toxocara canis on the ocular are difficult to study in human body. This study was designed to explore long-term ocular manifestation in multiple types of animals infected with Toxocara canis, uncovering the specific pathological mechanism, migration pathway of larvae after infection. Three experinment animals including mice, gerbils and rats were selected and infected with Toxocara eggs, and ocular changes were closely observed and recorded for at least 2 months. The ocular infection rate and mortality tended to be stable at 7 day-post-infection (dpi). At 7dpi, the achievement ratio of mice infected with 2000 T. canis eggs (M2k), gerbils infected with 1000 (G1k), 2000 (G2k) and 4000 (G4k) T. canis eggs were higher, respectively 62.5%, 75%, 100% and 100%. Hemorrhagic lesions, mechanical injury of retina and larval migration can be observed after 3dpi. And the larval tissue, the structure disorder and the inflammation could be observed in the pathological sections. In conclusion, the mice infected with 2000 T. canis eggs and gerbils infected with 1000, 2000, 4000 T.canis eggs represented obvious ocular lesions, could be served for long-term observation.
A Cross-Sectional Study on Health-Related Quality of Life, Sleep, and Psychiatric Disorders in Individuals with Pelvic and Acetabulum Fractures
Authors: ALI KHAN AKHTAR , He LINGXIAO , LI Wenkai , Zhang Weikai , Huang Hui 
Published: September 2023

Abstract
Purpose: The purpose of this study is to assess the long-term (4 years) health-related quality of life (HRQOL), sleep disorders, anxiety, depression, and psychiatric disorders in individuals with pelvic and acetabulum fractures through a cross-sectional study design.
Methods: A study was conducted on 265 patients with pelvic and acetabulum fractures from 2018 to 2022. The study investigated factors such as age, gender, cause of injury, hospital stay, blood loss, surgery duration, fracture reduction, surgical procedures, complications, and injury severity. The patient's quality of life was evaluated using EuroQoL-5D, VAS, and MPS, and sleep and psychological disorders were also assessed.
Results: In a 2-year study of 216 patients with pelvic and acetabulum fractures, 6.6% experienced borderline clinical depression and 45.2% reported mild mood disturbance. Anxiety was mild to moderate in 46% of Tile C and posterior acetabulum wall fracture patients. Insomnia and sleep movement disorders were reported by 24.8% and 23.1% of patients, respectively, but no significant correlation was found between fracture types and sleep disorders. Mean MPS and EQ-5D vas scores were 89.68 and 10.04, respectively. Differences in fracture types were revealed through logistic regression analysis, except for two MPS subscales.
Conclusions. We found that 80% of patients with pelvic and acetabular fractures had improved functional outcomes and quality of life. Older patients may experience anxiety and depression, as well as numbness or irritation related to implants. Posterior acetabulum wall fractures and Tile-C pelvic fractures require careful monitoring due to associated mild pain.
Risk Factors for Atrial Fibrillation after Video-assisted Thoracoscopic Surgery for Lung Cancer
Authors: Ruan Yingding , Han Jianwei , Yang Aiming , Ding Qingguo , Xu Jincheng , Zhang Ting 
Published: September 2023

Abstract
Background
This study aimed to analyze risk factors for postoperative atrial fibrillation (POAF) in video-assisted thoracoscopic surgery (VATS) for non-small cell lung cancer (NSCLC) resection.
Methods
A retrospective analysis was performed on a cohort of patients who underwent VATS for NSCLC resection at our hospital between January 2016 to October 2019. Patient demographics, preoperative data, and postoperative outcomes were collected and reviewed. Univariate and multivariate analyses were performed to identify significant risk factors for POAF.
Results
Among 276 NSCLC VATS patients, 29 developed POAF during hospitalization without in-hospital deaths. 25 cases of POAF emerged within the initial three postoperative days. With a cutoff age of 73, exceeding this age resulted in notably higher POAF incidence compared to non-POAF (10 vs. 46, P = 0.045). Additionally, POAF group had significantly more retrieved mediastinal lymph nodes (P = 0.011) and explored node stations (P = 0.005) compared to non-POAF. Intraoperative bleeding volume (P = 0.034) and postoperative pulmonary infections (P = 0.001) were also higher in POAF group. Preoperative hemoglobin levels differed significantly between POAF and non-POAF (P = 0.024). POAF group experienced extended postoperative hospitalization (P = 0.024) and drainage time (P = 0.011) compared to non-POAF. Multivariate analysis identified postoperative pulmonary infection as independent POAF risk factor following NSCLC VATS.
Conclusion
Our study revealed that pulmonary infection was one of the independent risk factors for POAF following VATS in patients with NSCLC. Furthermore, POAF is associated with prolonged drainage time and extended postoperative hospitalization.
Trial Registration
NO
Cellulose reinforced carbon nanocomposite hydrogels for wearable sensors
Authors: meng lingling , ding shijie , li weihao , liu da , liu en 
Published: September 2023

Abstract
he application of hydrogel in the field of wearable flexible sensors has been widely discussed. However, most hydrogels have some defects in mechanics, stability, and self-recovery, which greatly limit their application in flexible sensors. In this paper, carbon nanotube composite hydrogels with good stability and high stretchability were prepared by the one-pot method in water/glycerol binary solvent using acrylamide (AM), cellulose nanocrystals (CNC) and multi-walled carbon nanotubes (MWCNTs) as raw materials. The composite hydrogels were characterized by scanning electron microscopy and Fourier transform infrared, and their mechanics, conductivity, and stability were tested. The results show that when the content of cellulose nanocrystals (CNC) is 1 wt%, the composite hydrogel has a higher elongation at break (650.96%) and a higher tensile strength (0.26 MPa). This is due to the presence of hydrogen bonds, and the conductivity of the composite hydrogel can be improved with the addition of carbon nanotubes (MWCNTs). When 0.04 g of carbon nanotubes (MWCNTs) were added, the conductivity of the composite hydrogel reached 0.244 S/m, showing good conductivity. In addition, the wearable flexible sensor based on the composite hydrogel has a measurement factor of 7.33 (strain 400%), which can detect human movement. Therefore, carbon nanotube composite hydrogels with good stability and high stretchability have broad application prospects in the field of wearable flexible sensors.
Shotgun Metagenomic insights into the Plastisphere microbiome: Unveiling potential for clinical and industrial enzymes production along with plastic degradation
Authors: Saleem Mahnoor , Ali Akhtar , Razzak Safina Abdul , Khawaja Shariqa , Yahya Saira 
Published: September 2023

Abstract
Plastic pollution is one of the most resilient types of pollution, considered a global environmental threat, particularly in the marine environment. This study aimed to identify plastic-degrading bacteria from the plastisphere and their pharmaceutical and therapeutic potential. We collected samples from soil and aquatic plastisphere to identify the bacterial communities using shotgun metagenomic sequencing and bioinformatic tools. Results showed that the microbiome comprised 93% bacteria, 0.29% archaea, and 3.87% unidentified microbes. Of these 93% of bacteria, 54% were proteobacteria, 23.9% were firmicutes, 13% were actinobacteria, and 2.1% were other phyla. We found that the plastisphere microbiome was involved in degrading synthetic and polyhydroxyalkanoate (PHA) plastic, biosurfactant production, and can thrive under high temperatures. However, except Pseudomonas, no association existed between thermophiles, synthetic plastic or PHA degraders, and biosurfactant-producing bacterial species. Other plastisphere inhabiting plastic degrading microbes include Streptomyces, Bacillus, Achromobacter, Azospirillum, Bacillus, Brevundimonas, Clostridium, Paenibacillus, Rhodococcus, Serratia, Staphylococcus, Thermobifida, and Thermomonospora. However, the plastisphere microbiome showed potential for producing secondary metabolites that were found to act as anticancer, antitumor, anti-inflammatory, antimicrobial, and enzyme stabilizers under harsh environments. These results revealed that the plastisphere microbiome upholds clinical and environmental significance as it can open future portals in a multi-directional way.
The Gender Dimension of Vulnerability in Disaster Caused by the Corona Virus (Covid-19)
Authors: Cvetković Vladimir M. , Čvorović Milica , Beriša Hatidža 
Published: September 2023

Abstract
The subject of the research is an examination of the way in which the disease COVID-19 affects the gender aspects of the vulnerability of citizens, with special reference to their physical, socio-economic and psychological vulnerability. For the purposes of this research, the perception of risk and the preparedness of men and women for the SARS-CoV-2 virus pandemic was examined. The results of this research were obtained by applying a quantitative survey of the citizens of the Republic of Serbia about their experiences and attitudes regarding their vulnerability due to the COVID-19 pandemic, where special attention is paid to the gender dimension. A survey of 273 adult citizens was conducted and the results showed that there is no significant connection between the gender category and the physical vulnerability of people caused by the COVID-19 pandemic. The obtained data indicate that approximately the same percentage of women and men had severe symptoms of the disease. Nevertheless, the results showed that there is a partial connection between the gender category and socio-economic vulnerability, as well as a clear connection between the gender category and the psychological vulnerability of people caused by the pandemic. In both cases, the results indicate that women are more vulnerable socio-economically and psychologically than men. As this topic is not sufficiently represented in foreign, and especially not in domestic literature, the results of this research can serve as guidelines for future investigations of the gender dimension of people in the conditions of disasters, such as the COVID-19 pandemic.
Improving Antimicrobial Treatment in Terms of Antimicrobial Stewardship and Health Costs by an Opat Service a Single Centre Prospective Cohort Study
Authors: Burch Andrea R. , Ledergerber Bruno , Ringer Martin , Padrutt Maria , Reiber Claudine , Mayer Fabienne , Zinkernagel Annelies S. , Eberhard Nadia , Kaelin Marisa B. , Hasse Barbara 
Published: September 2023

Abstract
Purpose: Outpatient parenteral antimicrobial therapy (OPAT) is a standard for antimicrobial therapy internationally. With this prospective cohort study, we aimed to assess the impact of an OPAT service as part of antimicrobial stewardship (ABS) and evaluate the safety and efficiency of the program while illuminating the financial benefit for the hospital.
Methods: Socio-demographic data, treatment regimen and outcomes were prospectively recorded for all patients assigned to the program of the OPAT unit of the University Hospital of Zurich between November 2018 and September 2022.
Results: In total, we recorded 303 OPAT assignments of which 260 resulted in effective OPAT episodes. The 260 OPAT episodes were further optimized towards the choice of antimicrobial substance (n=18) and length of therapy (n=6). Moreover, OPAT resulted in alteration of patient assessment and care led by ABS strategies in 247 of 260 episodes (95%). While the bed days saved per year increased consistently with time, a total of 3934 in-hospital treatment days were saved amounting to a cost saving of 9’835’000 CHF over 47 months. Adverse events were recorded in 46 cases whilst only two of these have been the reason for readmission during OPAT treatment. Clinical cure was noted in 77% (199/260) and was negatively associated with Charlson Comorbidity Index (CCI; OR per 1 unit higher 0.85 (95% CI 0.78-0.93)).
Conclusion: This study demonstrates the impact of an OPAT service in the framework of ABS as well as its benefits for the hospital whilst preserving safety and efficacy for the patient’s parenteral antimicrobial treatment.
Hubungan Pola Perilaku Pengunjung dengan Karakteristik Arsitektur Istana Datu Luwu Kota Palopo
Authors: Rizaldy Mohammad , Wikantari Ria , Sir Mohammad Mochsen 
Published: September 2023

Title: The Relationship between Visitor Behavior Patterns and the Architectural Characteristics of Datu Luwu Palace, Palopo City

The research aims to find the relationship between visitor behavior patterns and the architectural characteristics of the Datu Luwu Palace in Palopo City. Datu Luwu Palace has important historical and cultural significance as a historical building complex in South Sulawesi. Factors such as the layout, visual design, and architectural elements within the palace can influence visitor behavior patterns. The research used a qualitative approach with a survey of visitors. Data were analyzed using spatial techniques to identify visitor behavior patterns and their relationship to the architectural characteristics of the palace. The results show a significant relationship between visitor behavior patterns, visit time, activities, and space preferences with the architectural characteristics of the Datu Luwu Palace. Architectural characteristics, such as traditional ornamentation, room layout, and materials, play an important role in shaping visitors' experiences and their activities within the palace. Research provides new insights into the management and development of historic tourist destinations. By better understanding the influence of architectural characteristics on visitor behavior patterns, tourism authorities and managers can take more effective steps to maintain, preserve, and enhance the visitor experience. The results of the research can be the basis for better planning in maintaining historical and cultural values, and on the other hand, fulfilling visitors' expectations in exploring tourist destinations. Research has a positive impact on the development and promotion of South Sulawesi's cultural heritage.
Predicting rainfall response to climate change and uncertainty analysis: Introducing a novel downscaling CMIP6 models technique based on the stacking ensemble machine learning
Authors: Anaraki Mahdi Valikhan , Kadkhodazadeh Mojtaba , Morshed-Bozorgdel Amirreza , Farzin Saeed 
Published: September 2023

Abstract

This study proposes a novel downscaling technique based on stacking ensemble machine learning (SEML) to predict rainfall under climate change. The SEML consists of two levels. Rainfall time series predicted by level 1 algorithms MLR, MNLR, MARS, M5, RF, LSBoost, LSSVM-GS, and a novel hybrid algorithm namely LSSVM-RUN) are used as inputs to the level 2 machine learning algorithm (MARS and LSSVM_RUN). Then, meta-algorithms of SEML predict rainfall based on eight predicted rainfall in level 1. This approach boosts prediction accuracy by utilizing the strong points of different machine learning (ML) algorithms. Results showed that MARS and LSSVM-RUN could be employed to improve the modeling results as meta-algorithms (level 2 of the SEML). Three global climate models (GCMs) in the historical period (1985–2014) and three SSP scenarios in the future period (2021–2050) were considered for downscaling and predicting rainfall at Lake Urmia and Sefidrood basins. Using meta-algorithms, the prediction results showed that rainfall in all scenarios and stations decreased between 0.02 and 0.20% (except Takab station in model CanESM5 scenarios). Hence, the proposed stacking ensemble ML has the potential for modeling and predicting precipitation with good accuracy and high reliability.
Short- to Long-Term Effects of Virtual Reality on Motor Skill Learning in Children With Cerebral Palsy: Systematic Review and Meta-Analysis
Authors: Kilcioglu Seyma , Schiltz Benoît , Araneda Rodrigo , Bleyenheuft Yannick 
Published: September 2023

Background
Many studies have started integrating virtual reality (VR) into neurorehabilitation for children with cerebral palsy (CP). The results of the effects of VR on motor skill learning, including the short- to long-term results of relevant studies, must be pooled in a generic framework.

Objective
This systematic review and meta-analysis aimed to investigate the short- to long-term effects of therapies including VR on motor skill learning in children with CP.

Methods
Two examiners followed the inclusion and exclusion criteria of the “Participant, Intervention, Control, and Outcome” framework. Randomized controlled trials (RCTs) and non-RCTs were considered if they compared VR-included interventions with control groups on motor functions and daily life activities in children with CP. PubMed, ScienceDirect, Embase, and IEEE Xplore databases were searched. The modified Downs and Black assessment was used to assess the methodological quality of the included studies. Meta-analyses and subgroup analyses for RCTs were conducted whenever possible.

Results
A total of 7 RCTs, 2 non-RCTs, and 258 children with CP were included. The priority focus of 78% (7/9) of the studies was upper limb functions. There was a significant short-term effect of adding VR to conventional therapies on upper limb functions when compared with conventional therapies (P=.04; standardized mean difference [SMD]=0.39, 95% CI 0.01-0.76). The overall medium- to long-term effects showed a trend toward favoring the VR group, although the difference was not statistically significant (P=.06; SMD=0.37, 95% CI −0.02 to 0.77). For balance (P=.06; SMD=1.04, 95% CI −0.04 to 2.12), gross motor functions (P=.30; SMD=2.85, 95% CI −2.57 to 8.28), and daily life activities outcomes (P=.21; SMD=0.29, 95% CI −0.16 to 0.74), the overall effect in the short term also showed a trend toward favoring the VR group, but these results were not statistically significant.

Conclusions
VR seems to have additional benefits for motor skill learning in children with CP. Studies with follow-up outcomes of VR training focusing on balance and gross motor functions in patients with CP were quite limited. Future research on balance and gross motor function outcomes should target particularly long-term results of therapies including VR on motor skill learning.

Trial Registration
PROSPERO International Prospective Register of Systematic Reviews CRD42021227734; https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021227734
Construction of an Emotional Lexicon of Patients With Breast Cancer: Development and Sentiment Analysis
Authors: Li Chaixiu , Fu Jiaqi , Lai Jie , Sun Lijun , Zhou Chunlan , Li Wenji , Jian Biao , Deng Shisi , Zhang Yujie , Guo Zihan , Liu Yusheng , Zhou Yanni , Xie Shihui , Hou Mingyue , Wang Ru , Chen Qinjie , Wu Yanni 
Published: September 2023

Background
The innovative method of sentiment analysis based on an emotional lexicon shows prominent advantages in capturing emotional information, such as individual attitudes, experiences, and needs, which provides a new perspective and method for emotion recognition and management for patients with breast cancer (BC). However, at present, sentiment analysis in the field of BC is limited, and there is no emotional lexicon for this field. Therefore, it is necessary to construct an emotional lexicon that conforms to the characteristics of patients with BC so as to provide a new tool for accurate identification and analysis of the patients’ emotions and a new method for their personalized emotion management.

Objective
This study aimed to construct an emotional lexicon of patients with BC.

Methods
Emotional words were obtained by merging the words in 2 general sentiment lexicons, the Chinese Linguistic Inquiry and Word Count (C-LIWC) and HowNet, and the words in text corpora acquired from patients with BC via Weibo, semistructured interviews, and expressive writing. The lexicon was constructed using manual annotation and classification under the guidance of Russell’s valence-arousal space. Ekman’s basic emotional categories, Lazarus’ cognitive appraisal theory of emotion, and a qualitative text analysis based on the text corpora of patients with BC were combined to determine the fine-grained emotional categories of the lexicon we constructed. Precision, recall, and the F1-score were used to evaluate the lexicon’s performance.

Results
The text corpora collected from patients in different stages of BC included 150 written materials, 17 interviews, and 6689 original posts and comments from Weibo, with a total of 1,923,593 Chinese characters. The emotional lexicon of patients with BC contained 9357 words and covered 8 fine-grained emotional categories: joy, anger, sadness, fear, disgust, surprise, somatic symptoms, and BC terminology. Experimental results showed that precision, recall, and the F1-score of positive emotional words were 98.42%, 99.73%, and 99.07%, respectively, and those of negative emotional words were 99.73%, 98.38%, and 99.05%, respectively, which all significantly outperformed the C-LIWC and HowNet.

Conclusions
The emotional lexicon with fine-grained emotional categories conforms to the characteristics of patients with BC. Its performance related to identifying and classifying domain-specific emotional words in BC is better compared to the C-LIWC and HowNet. This lexicon not only provides a new tool for sentiment analysis in the field of BC but also provides a new perspective for recognizing the specific emotional state and needs of patients with BC and formulating tailored emotional management plans.