bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virtual implant scan body switch by using computer‐aided design programs avoiding the need of obtaining a new intraoral implant digital scan: A novel protocol
Authors: Alonso Pérez‐Barquero Jorge , García‐Sala Bonmatí Fernando , de Fernández Estevan Lucía , Guinot Barona Clara , Maneiro Lojo Jesús , Revilla‐León Marta 
Published: September 2023

AbstractObjectiveThe present manuscript describes a technique to virtually switch an implant scan body eliminating the need of obtaining a new intraoral implant digital scan.Clinical ConsiderationsImplant scan bodies assist on transferring the 3‐dimensional position of the implants into the virtual definitive implant cast. However, if a different implant part is desired during the designing procedures of the implant restoration such as selecting a different implant abutment of varying height, angulation, or manufacturer, a new intraoral implant digital scan with the specific implant scan body is required.ConclusionsThis novel protocol aims to reduce possible complications that require capturing a new intraoral implant digital scan, facilitate prostheses design modifications after the obtention of the definitive intraoral implant digital scan, and to ease the manufacturing procedures.Clinical SignificanceThe novel technique may provide a solution for virtually switch implant scan bodies for fabricating implant‐supported single crowns or short‐span prostheses. Additional studies are needed before its clinical implementation.
Cerebrospinal fluid and blood serum biomarkers in neurodegenerative proteinopathies: A prospective, open, cross‐correlation study
Authors: Koníčková Dorota , Menšíková Kateřina , Klíčová Kateřina , Chudáčková Monika , Kaiserová Michaela , Přikrylová Hana , Otruba Pavel , Nevrlý Martin , Hluštík Petr , Hényková Eva , Kaleta Michal , Friedecký David , Matěj Radoslav , Strnad Miroslav , Novák Ondřej , Plíhalová Lucie , Rosales Raymond , Colosimo Carlo , Kaňovský Petr 
Published: September 2023

AbstractNeurodegenerative diseases are a broad heterogeneous group affecting the nervous system. They are characterized, from a pathophysiological perspective, by the selective involvement of a subpopulation of nerve cells with a consequent clinical picture of a disease. Clinical diagnoses of neurodegenerative diseases are quite challenging and often not completely accurate because of their marked heterogeneity and frequently overlapping clinical pictures. Efforts are being made to define sufficiently specific and sensitive markers for individual neurodegenerative diseases or groups of diseases in order to increase the accuracy and speed of clinical diagnosis. Thus said, this present research aimed to identify biomarkers in the cerebrospinal fluid (CSF) and serum (α‐synuclein [α‐syn], tau protein [t‐tau], phosphorylated tau protein [p‐tau], β‐amyloid [Aβ], clusterin, chromogranin A [chromogrA], cystatin C [cyst C], neurofilament heavy chains [NFH], phosphorylated form of neurofilament heavy chains [pNF‐H], and ratio of tau protein/amyloid beta [Ind tau/Aβ]) that could help in the differential diagnosis and differentiation of the defined groups of α‐synucleinopathies and four‐repeat (4R‐) tauopathies characterized by tau protein isoforms with four microtubule‐binding domains. In this study, we analyzed a cohort of 229 patients divided into four groups: (1) Parkinson's disease (PD) + dementia with Lewy bodies (DLB) (n = 82), (2) multiple system atrophy (MSA) (n = 25), (3) progressive supranuclear palsy (PSP) + corticobasal syndrome (CBS) (n = 30), and (4) healthy controls (HC) (n = 92). We also focused on analyzing the biomarkers in relation to each other with the intention of determining whether they are useful in distinguishing among individual proteinopathies. Our results indicate that the proposed set of biomarkers, when evaluated in CSF, is likely to be useful for the differential diagnosis of MSA versus 4RT. However, these biomarkers do not seem to provide any useful diagnostic information when assessed in blood serum.image
Randomized controlled trial of hydrolyzed fish diets in dogs with chronic enteropathy
Authors: Simpson Kenneth W. , Miller Meredith L. , Loftus John P. , Rishniw Mark , Frederick Carol E. , Wakshlag Joseph J. 
Published: September 2023

AbstractBackgroundThe role of diet in the pathogenesis and treatment of chronic enteropathies (CE) in dogs is unresolved.ObjectivesTo compare the ability of diets composed of hydrolyzed fish, rice starch, and fish oil without (HF) or with prebiotics, turmeric, and high cobalamin (HF+) against a limited ingredient diet containing mixed nonhydrolyzed antigens and oils (control) to resolve clinical signs and maintain serum cobalamin and folate concentrations in dogs with nonprotein losing CE (non‐PLE). To determine the ability of hydrolyzed fish diets to support recovery and remission in dogs with PLE.AnimalsThirty‐one client‐owned dogs with CE: 23 non‐PLE, 8 PLE.MethodsRandomized, blinded, controlled trial. Diets were fed for 2 weeks; responders continued for 12 weeks. Nonresponders were crossed over to another diet for 12 weeks. Response was determined by standardized clinical evaluation with long‐term follow‐up at 26 weeks. Concurrent medications were allowed in PLE.ResultsNineteen of 23 (83%; 95% confidence interval [CI], 60%‐94%) non‐PLE CE responded clinically to their initial diet, with no difference between diets (P > .05). Four nonresponders responded to another diet, with sustained remission of 18/18 (100%; 95%CI, 78%‐100%) at 26 weeks. Serum cobalamin concentration was increased (P < .05) and maintained by diet. Serum folate concentration decreased posttreatment (P < .05) but was restored by dietary supplementation. Hydrolyzed fish diets supported weight gain, serum albumin concentration, and recovery (P < .05) in dogs with PLE.Conclusions and Clinical ImportanceChanging diet, independent of antigen restriction or supplemental ingredients, induced long‐term remission in dogs with non‐PLE CE. Serum cobalamin and folate concentrations were maintained by diet. Hydrolyzed fish diets supported clinical recovery and remission in PLE.
Perinatal dietary patterns and symptomatic depression: A prospective cohort study
Authors: Chan Lin‐Chien , Wang Hsiu‐Hui , Wahlqvist Mark L. , Liu Cheng‐Chieh , Liu Jah‐Yao , Lee Meei‐Shyuan 
Published: September 2023

AbstractTo promote maternal and infant health, there is a need to optimise the dietary pattern of pregnant women to reduce perinatal depression. This prospective cohort study was conducted from June 2020 to February 2022, 300 women from a medical center were interviewed during late pregnancy and at 4–6 weeks postpartum. Dietary patterns were derived by factor analysis using a semiquantitative food frequency questionnaire. Symptomatic depression was defined using the Edinburgh Postpartum Depression Scale (EPDS, ranged 0–30). Their dairy, vegetable and fruit intakes were below the Taiwanese recommendations for pregnant women. Symptomatic depression (EPDS ≥10) affected 31.3% in the third trimester and 35.7% postpartum. Pre‐ and post‐EPDS scores were positively correlated (r = 0.386, p < 0.001). Approximately 55% of those depressed before delivery were also depressed postpartum. For late pregnancy, four dietary patterns were identified (‘Good oil’, ‘Vegetables and fruits’, ‘Omnivorous’ and ‘Refined‐grain and organ meats’). Dietary patterns were classified according to quartiles (Q). Higher omnivorous pattern scores reduced the risk of depression. For prenatal depression, with Q1 as a reference, the risk was reduced by 38% for Q2, 43% for Q3 and 59% for Q4 (p for trend = 0.068). These findings became evident postpartum (reduced risk by 68% for Q2, 69% for Q3 and 70% for Q4 (p = 0.031; p for trend = 0.0032). The association between dietary patterns and depression encourages the routine nutritional management of pregnant women.
Not All Osteophytes Are Located on the Right Side of the Vertebrae in Diffuse Idiopathic Skeletal Hyperostosis: A Quantitative Analysis in Relation to the Position of Aorta
Authors: Chen Haojie , Zhou Qingshuang , Wang Sinian , Pu Xiaojiang , Zhou Haicheng , Wang Bin , Zhu Zezhang , Qiu Yong , Sun Xu 
Published: September 2023

ObjectiveDiffuse idiopathic skeletal hyperostosis (DISH) is characterized by osteophytes in the anterior vertebrae, and the presence of aorta may have an impact on their formation. However, the anatomical positional relationship between the aorta and osteophytes in patients with DISH remains controversial. This study aimed to evaluate the position of osteophytes in relation to aorta in DISH, and the influence of aortic pulsation on the formation of osteophytes from the perspective of morphology.MethodsWe conducted a retrospective review of 101 patients diagnosed with DISH and symptomatic lumbar spinal stenosis between June 2018 and December 2021. A total of 637 segments with heterotopic ossification in DISH were used for quantitative measurements on CT scans. The Cartesian coordinate system was built up on the axial CT scans to reflect the relative position between aorta and osteophytes. Osteophytes were divided into adjacent aorta group (AD group) and non‐adjacent aorta group (N‐AD group). In terms of the morphology, osteophytes in the AD group were further divided into convex, flat, and concave types. The relative position between aorta and osteophytes, and the aorta‐osteophyte distance and morphology of osteophytes were compared. Univariate analysis of variance was performed for multiple groups, and two independent‐samples t‐tests were used for two groups.ResultsFrom T5 to L4, aorta gradually descended from left side to middle of vertebrae, and osteophytes gradually shifted from right side of vertebrae (T5‐T10) to bilateral sides (T11‐L4). Of 637 osteophytes in DISH, 60.1% (383/637) were in AD group, including convex type 0.6% (4/637), flat type 34.7% (221/637), and concave type 24.8% (158/637). The N‐AD group accounted for 39.9% (254/637). Flat osteophytes were concentrated in T5‐T12, while concave osteophytes in T11‐L4. Overall, the aorta‐osteophyte distance of concave type was significantly smaller than that of flat type.ConclusionOsteophytes are not always located on the right side of vertebrae, but move with the position of the descending aorta. Furthermore, the morphology of osteophytes varies by vertebral segment in DISH, which is related to aorta descending anteriorly in the spine.
Petiole XLA (xylem to leaf area ratio) integrates hydraulic safety and efficiency across a diverse group of eucalypt leaves
Authors: Blackman Chris J. , Halliwell Ben , Hartill Gabrielle E. , Brodribb Timothy J. 
Published: September 2023

AbstractA theoretical trade‐off between the efficiency and safety of water transport systems in plants is used to explain diverse ecological patterns, from tree size to community structure. Despite its pervasive influence, this theory has marginal empirical support. This may be partially due to obfuscation of associations by wide phylogenetic sampling or non‐standard sampling between studies. To address this, we examine the coordination of structural and anatomical traits linked to hydraulic safety and efficiency in the leaves of an ecologically diverse group of eucalypts. We introduce a new trait for characterising leaf water transport function measured as the cross‐sectional XA at the petiole divided by the downstream leaf area (XLApetiole). Variation in XLApetiole revealed support for a safety‐efficiency trade‐off in eucalypt leaves. XLApetiole was negatively correlated with theoretical petiole xylem conductivity (Ks_petiole) and strongly negatively correlated with leaf cavitation vulnerability (Ψ50leaf). Species with lower Ψ50leaf exhibited petiole xylem with narrower vessels and greater fibre wall area fractions. Our findings highlight XLApetiole as a novel integrative trait that provides insights into the evolution of leaf form and function in eucalypts and holds promise for wider use among diverse species.
Iodixanol supplementation in freezing extender improves the antioxidant capacity of semen
Authors: Marqui Fernanda N. , Martins Alicio , da Cruz Tairini Erica , Berton Tatiana Issa Uherara , de Paula Freitas‐Dell’Aqua Camila , Dell’Aqua José A. , Oba Eunice 
Published: September 2023

AbstractThe aim of this study was to evaluate the effect of supplementing bovine semen freezing extender with different concentrations of iodixanol on post‐thaw sperm characteristics. Six ejaculates of three Nellore bulls were pooled and diluted in commercial extender (BotuBov®) and then divided into 4 groups: control group (without adding iodixanol); groups G1.5, G3, or G6 according to the concentration of iodixanol solution (RedCushion®). After dilution, the samples were cooled and frozen. Post‐thaw semen evaluation included sperm motility by CASA immediately after thawing and after 60 min of incubation at 37°C, flow cytometry analysis for integrity of plasma and acrosomal membranes, membrane destabilization and translocation of phosphatidylserine, mitochondrial membrane potential, and formation of intracellular anion superoxide (), hydrogen peroxide (H2O2), and membrane lipid peroxidation. The group G6 presented significantly higher (p < .05) total and progressive motility, percentage of plasma and acrosomal membrane integrity, and H2O2than control and group G1.5. Furthermore, group G6 showed lower (p < .05) lipid peroxidation than control. In addition, regardless of the concentration used, the percentage of spermatozoa without phosphatidylserine translocation was higher (p < .05) in all iodixanol supplemented groups. In conclusion, iodixanol supplementation preserved the motility and integrity of sperm membranes during cryopreservation and protected against lipid peroxidation.
Teaching digital interprofessional collaboration
Authors: Cresswell Nikki Rose , Walker Steven Thomas , Harrison Claire , Kent Fiona 
Published: September 2023

AbstractBackgroundInterprofessional communication in Australian clinical practice has recently shifted from paper records to electronic medical records (EMRs), and there is therefore a need for universities to prepare health graduates for digital communication.ApproachAn interprofessional team co‐designed and co‐facilitated an online interprofessional activity for third year pharmacy and fifth year medical students utilising a simulated EMR (EHR Go™). Students completed a series of profession‐specific tasks relating to a simulated patient, then came together in interprofessional groups of 10–12 to establish a collaborative medication charting plan.EvaluationA total of 640 students participated in the activity, of which 60% (386/640) were medical students. Immediately after the interprofessional workshop, students were invited to complete a voluntary anonymous evaluation. Five‐point Likert scales were used to rate the relevance to practice, achievement of learning outcomes, organisation and overall quality of the activity. Students were also asked to contribute qualitative feedback. Of the participants, 28% (180/640) completed the survey, and 53% (96/180) of respondents were medical students. A majority of survey respondents agreed or strongly agreed that the activity was relevant to practice (94%; 170/180), achieved the learning outcomes (84%; 151/180), was well organised (74%; 133/180) and was a quality learning experience (79%; 143/180). The positive feedback focused on appreciating the opportunity to discuss with interdisciplinary colleagues the rationale for admission medications. Constructive feedback included the need for clearer directives regarding pre‐workshop activities.ImplicationsThis interprofessional education (IPE) activity presents a feasible, innovative approach to teaching pharmacy and medical students digital communication and collaboration using a simulated EMR. Overall, a majority of learners were satisfied with the learning experience and felt that the IPE activity achieved the learning outcomes and was relevant to practice.
Telehealth in paediatric physical therapy education: Strategies and perceptions of interns and caregivers of children with disabilities in Brazil
Authors: Sudati Isabella Pessóta , Monteiro Rayane Félix Lôbo , Nasser Ana Beatriz , Rocha Nelci Adriana Cicuto Ferreira , de Campos Ana Carolina 
Published: September 2023

AbstractAimsTo describe the implementation of paediatric rehabilitation telehealth at a physical therapy (PT) unit in Brazil during the COVID‐19 pandemic and to describe the perception about this modality by two groups: (1) undergraduate PT students using telehealth during their clinical rotations in this unit and (2) the caregivers of children with disabilities receiving the services.MethodsTwenty‐one PT interns (19 females; 25 ± 2 years of age) and seven caregivers (seven females; 40 ± 6 years of age) of seven children with disabilities (five females; 10 ± 4 years of age; five children diagnosed with cerebral palsy) responded to an online questionnaire about their experience with the telehealth programme. Participant attendance and frequency of objective responses were reported descriptively; open‐ended responses were analysed qualitatively and grouped according to broad themes.Results71.4% of interns rated telehealth as an excellent or good experience, and 28.6% did not appreciate it. In addition, 28.6% of them thought that telehealth should be part of the mandatory internship. Regarding caregivers, 85.8% judged the telehealth programme as excellent or good. Both interns and caregivers cited pros and cons related to technology, professional resources, communication skills and caregiver‐related aspects, among others.ConclusionsSpecific teaching strategies had to be utilised for implementation of telehealth. Despite being a novel modality for interns and caregivers, telehealth was well accepted.
Fast Meshless Solution With Lumped Friction for Laminar Fluid Transients
Authors: Xu Yuanzhi , Deng Yang , Jiao Zongxia 
Published: September 2023

Abstract
Fluid transients without friction in pipelines can be solved by a time-domain exact solution, using a simple recursive process without computational grid. However, the calculation time cost of this approach is very high because of the recursion algorithm. Its improved method, named as the fast meshless solution (FMS), was developed to speed up the computation by introducing time-line interpolation. However, when the friction is considered, the conventional distributed friction model cannot be employed directly for the FMS is meshless. To address this problem, the fluid friction is lumped at pipe ends, and both steady and unsteady laminar flow are investigated. Three kinds of lumped models are proposed here, and compared with a numerical case of the water hammer. The laminar fluid transients can be calculated quickly by the present method, with a little reduced accuracy. This method may be of interest in a quick assessment of the piping fluid transients.
Experimental Investigation of High Frequency Flame Response on Injector Coupling in a Perfectly Premixed Multi-Jet Combustor
Authors: Rosenkranz Jan-Andre , Sattelmayer Thomas 
Published: September 2023

Abstract
High frequency injector-coupled thermoacoustic instabilities are a major threat to multi-jet combustors in rocket and gas turbine engines. The complex three-dimensional acoustic coupling between the combustion chamber and injector acoustics cause local fluctuations in heat release. In turn, multiple thermoacoustic feedback mechanisms close the thermoacoustic loop and serve as a source of the thermoacoustic instability. Except for the flame deformation and flame displacement mechanism, the underlying feedback mechanisms for high frequency instabilities are to a large extent unknown. The paper at hand gives new insights into the injector-coupled convective driving mechanisms that are present in multi-jet combustors at perfectly premixed conditions. The forced flame response to the first transverse combustor mode is investigated for two distinct injector tube lengths: one with an axial acoustic velocity node and one with a velocity antinode coupling at the injector–combustor interface. Phase locked OH* images reveal convectively transported coherent vortex structures as the main source of the flame response. The origin of the flame response can be linked to the axial acoustic velocity at the injector–combustor interface using numerical simulations. Both configurations show a clear oscillation of the heat release fluctuations in-phase with the acoustic pressure fluctuations. In similarity to time delay models in low frequency thermoacoustics, a wave number model is proposed to estimate the local flame response due to feed flow modulations and validated with the experimental results.
An underlying mechanism for MleR activating the malolactic enzyme pathway to enhance acid tolerance in
Lacticaseibacillus paracasei
L9
Authors: Huan Ran , Cao Zeyu , Zhai Zhengyuan , Feng Xin , Hao Yanling 
Published: September 2023

ABSTRACT

Tolerance to acid stress is a crucial property of probiotics against gastric acids. The malolactic enzyme pathway is one of the most important acid resistance systems in lactic acid bacteria. It has been reported that the malolactic enzyme pathway was regulated by the transcriptional regulator, MleR. However, regulatory mechanisms underlying malolactic enzyme pathway to cope with acid stress remain unknown. In this study, the acid tolerance ability of the
ΔmleR
deletion strain was significantly lower than that of the wild-type strain, and the complementation of the
mleR
gene into the
ΔmleR
strain restored the acid tolerance of the
ΔmleR
strain, indicating that MleR was involved in acid tolerance response of
Lacticaseibacillus paracasei
L9. Real-time quantitative PCR and transcriptional fusion experiments confirmed MleR-activated transcription of the
mleST
gene cluster. Furthermore, MleR was confirmed to directly bind to the promoter region of the
mleST
operon using ChIP assays and EMSAs. The transcription start site G of the
mleST
operon was located at position −198 relative to the start codon of the
mleS
gene. The region from −80 to −61 upstream of the transcription start site was determined to be essential for MleR binding. Moreover, L-malic acid acted as an effector for MleR to activate the transcription of the
mleST
operon in a dose-dependent manner. These results revealed the regulatory mechanism behind MleR-mediated activation of the malolactic enzyme pathway to enhance acid tolerance in
Lc. paracasei
L9.

IMPORTANCE

Lacticaseibacillus paracasei
is extensively used as probiotics in human health and fermented dairy production. Following consumption,
Lc. paracasei
is exposed to a variety of physico-chemical stresses, such as low pH in the stomach and bile salts in the intestines. The high acidity of the stomach severely inhibits bacterial metabolism and growth. Therefore, the acid tolerance response is critical for
Lc. paracasei
to survive. It has been reported that the malolactic enzyme (MLE) pathway plays an important role for LAB to resist acid stress. However, the regulatory mechanism has not yet been investigated. In this study, we determined that the LysR-type regulator MleR positively regulated the MLE pathway to enhance acid tolerance by binding −80 to −61 upstream of the transcription start site of the
mleST
operon. Further, L-malic acid acts as a co-inducer for MleR transcriptional regulation. Our study provides novel insights into acid tolerance mechanisms in LAB.