bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract 725: Prostate cancer risk disparities and sociocultural factors in men of African ancestry
Authors: Brown Joan M. 
Published: April 2023

Abstract
Introduction: The purpose of this research study was to quantify the prostate cancer (PCa) mortality rate in Men of African Ancestry (MAA) living in the State of Florida, the tri-county region of South Florida including Broward, Palm Beach, and Miami-Dade, and to explore the embodiment of sociocultural factors that contribute to the PCa disparity in the United State (U.S.).
Method: A quantitative, non-experimental, descriptive study design was used to identify differences in the resident PCa mortality rate in MAA stratified based on single race of Black and birth origin of Haiti (HA), Jamaica (JA), or the United States (U.S.). The intersection of health disparities and the embodiment of chronic exposure to sociocultural stressors was also explored.
Results: The Paired t-Test was used for statistical testing. The results indicated death from PCa is a health disparity in MAA in Florida when examined in aggregate by ancestry and disaggregate by birth origin. In the state of Florida, when the PCa death rate was viewed in aggregate by ancestry, a significant difference was found between MAA and Men of European Ancestry (MEA) (t(2)=4.303, p≤.01). There was a statistically significant relationship found between being MAA and death from PCa (r=.866, p≤.01). This suggests there are place based health disparities among MAA in the State of Florida. When the PCa mortality rate in MAA in the tri county region of South Florida was examined in aggregate by ancestry versus disaggregate by birth origin a significant difference was shown between MAA and HA born men (t(2)=4.30, p=0.05). No difference was shown between MAA and U.S., or JA born men. By county, the highest death rate was found in JA born men in Miami-Dade, and HA born men in Broward and Palm Beach. Overall, the lowest death rates in each county were found in U.S. born men. The results suggest that there are clear placed based and birth origin disparities in the South Florida region.
Conclusions: Examining differences in the PCa mortality rates in MAA in disaggregate by birth origin provides opportunity for the observation of sociocultural factors that affect health such as cultural beliefs and fears, chronic exposure to stressors such as structural and explicit racism and racialization in the U.S, immigration status and barriers to healthcare; all sociocultural factors that if examined may aid in explaining the increased rate of PCa death experienced by MAA and why the disparity exists.
Citation Format: Joan M. Brown. Prostate cancer risk disparities and sociocultural factors in men of African ancestry [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 725.
Abstract 975: Treatment prognostic signature of patients with non-small cell lung cancer: a retrospective single-institutional study
Authors: Smith Margaret R. , Wang Yuezhu , D' Agostino Ralph , Liu Yin , Ruiz Jimmy , Lycan Thomas , Oliver George , Topaloglu Umit , Pinkney Jireh , Abdulhaleem Mohammed N. , Chan Michael D. , Farris Michael , Su Jing , Xing Fei 
Published: April 2023

Abstract
Introduction: Currently, several types of treatment can be used to treat non-small cell lung cancer (NSCLC) depending on a potential druggable mutation or stage of cancer. However, a limited number of biomarkers are available to guide clinicians in selecting the most effective therapy for all patients.
Methods: The clinical characteristics of 642 NSCLC patients and tumor sequencing data were collected retrospectively at Atrium Health Wake Forest Baptist. Cox-proportional hazard regression models were fit to identify mutations that were “beneficial” (hazard ratio &lt; 1) or “detrimental” (hazard ratio &gt; 1) for patients on different treatment regimens, followed by the generation of mutation composite scores (MCS) for each treatment. The overall survival (OS) of patients receiving each treatment was plotted based on the patients’ MCS, and receiver operating characteristics (ROC) curves tested the predictive power of each MCS for each treatment group. We also identified novel co-occurring and mutually exclusive mutations in each treatment group by mutation interaction analysis.
Results: We identified treatment-specific mutations associated with either a better or worse OS. The MCS generated for each treatment group significantly enhanced the prediction power compared to a single mutation with limited application in patients with rare mutations. Mutation signatures to chemotherapy (NTRK1, FBXW7, BRAF, MPL, KRAS, and GATA3) and immunotherapy (MAP2K1, EGFR, CDK4, NTRK1, and NOTCH1) have a comparable prediction power with actual clinical response.
Conclusions: NSCLC patients’ responses to specific treatments are diverse because of tumor heterogeneity. Our work demonstrates how analyzing patients’ sequencing data facilitates the clinical selection of optimized treatment strategies.
Citation Format: Margaret R. Smith, Yuezhu Wang, Ralph D' Agostino, Yin Liu, Jimmy Ruiz, Thomas Lycan, George Oliver, Umit Topaloglu, Jireh Pinkney, Mohammed N. Abdulhaleem, Michael D. Chan, Michael Farris, Jing Su, Fei Xing. Treatment prognostic signature of patients with non-small cell lung cancer: a retrospective single-institutional study [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 975.
Abstract 985: An innovative spatial biology biomarker discovery approach to enable acceleration of drug development
Authors: Reeves Jason W. , Anguiano Esperanza , Devgan Vikram , Confuorto Nicholas , Schmid Joachim , Liang Yan , Beechem Joseph 
Published: April 2023

Abstract
Biomarker development in the clinical trial setting is frequently limited by the availability of small tissue specimens, such as core needle biopsies. To enable forward and reverse translational assays to best understand patient-specified biomarkers, high-plex assays which can be performed on individual slides are critical. We’ve developed the GeoMx® Digital Spatial Profiler (DSP) and CosMx™ Spatial Molecular Imager (SMI) to allow researchers to explore more hypotheses simultaneously from standard histopathology slides. We demonstrate on standard FFPE sections how the GeoMx and CosMx platforms integrate within the context of biomarker discovery. The GeoMx platform can resolve transcriptome-wide RNA expression profiles or 150+ protein targets from tissue structures selected by pathological review. The CosMx platform allows subcellular profiling of up to 1000 RNA transcripts or 64 proteins from a single slide. We demonstrate the integration of GeoMx and CosMx into a multi-modal approach to how high-plex spatial platforms can complement conventional diagnostic assays for translational research. This includes integration with downstream analytical tools such as digital pathology suites as well as machine learning approaches to model patient outcomes. Spatial technologies, such as GeoMx and CosMx, present groundbreaking opportunities for improving accuracy in biomarker discovery and for providing rich biological insight beyond low-plex profiling approaches.
Citation Format: Jason W. Reeves, Esperanza Anguiano, Vikram Devgan, Nicholas Confuorto, Joachim Schmid, Yan Liang, Joseph Beechem. An innovative spatial biology biomarker discovery approach to enable acceleration of drug development [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 985.
Abstract 991: Leveraging sequences missing from the human genome to detect cancer
Authors: Barnea Ofer Yizhar , Georgakopoulos-Soares Ilias , Ahituv Nadav , Chapman Jocelyn , Hemberg Martin , Mouratidis Ioannis , Syrigos Konstantinos , Syrigos Nikolaos , Vathiotis Ioannis , Mahajan Mayank , Panagiotou Emmanouil , Charpidou Andriani , Kvale Mark , Chan Candace S. , Easterlin Ryder 
Published: April 2023

Abstract
Introduction: Cancer diagnosis using cell-free DNA (cfDNA) has the potential to improve treatment and survival but has several technical limitations.
Methods: Here, we used neomers, short DNA sequences (13-17bp in length) that are largely absent from the healthy human genome, but appear in the tumor genome due to somatic mutations, to detect cancer at early stages from cfDNA. cfDNA was extracted from 1mL of plasma, and utilized from Whole Genome Sequencing at 5X coverage.
Results: First, we analyzed over 2,500 cancer whole-genome sequences (WGS), to show that a neomer-based classifier can distinguish twenty-one different tumor types with higher accuracy than state-of-the-art methods. Refinement of this classifier via manually selected neomer sets, identified additional cancer features with greater precision. We next generated cfDNA WGS data from &gt;600 patients and non-cancerous controls across four different types of cancers (prostate, lung, ovarian, colorectal), and demonstrated that neomers can precisely identify cancer from a limited amount of plasma for all four cancer types, with exampled sensitivities of 93.73% and 73.47% for lung and ovarian cancers, respectively. Additionally, using both luciferase and massively parallel reporter assays (MPRAs), we show that our neomer-driven approach can detect cancer-associated non-coding mutations that affect gene regulatory activity.
Conclusions: Our results show that neomers provide a novel, sensitive, specific and potentially broadly applicable cancer detection tool. This approach has several advantages over existing strategies for liquid biopsies: 1) it is based on standard next-generation sequencing which is already widely available in the clinic; 2) it requires low amounts of starting material and is completely tumor agnostic; 3) it searches the entire genome and is not restricted to known driver mutations. 4) it allows the detection of early-stage disease.
Citation Format: Ofer Yizhar Barnea, Ilias Georgakopoulos-Soares, Nadav Ahituv, Jocelyn Chapman, Martin Hemberg, Ioannis Mouratidis, Konstantinos Syrigos, Nikolaos Syrigos, Ioannis Vathiotis, Mayank Mahajan, Emmanouil Panagiotou, Andriani Charpidou, Mark Kvale, Candace S. Chan, Ryder Easterlin. Leveraging sequences missing from the human genome to detect cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 991.
Abstract PL04-05: LAG3: The third checkpoint inhibitor
Authors: Vignali Dario A. 
Published: April 2023

Abstract
Immunotherapies targeting the PD1/PDL1 pathway have had a major impact on cancer treatment. However, only a proportion of patients respond, and an even smaller proportion exhibit a long-term, durable cure. Several mechanisms of resistance and potential combinatorial approaches will be discussed. Lack of response to inhibitory receptor (IR) blockade therapy and increased disease burden has been associated with circulating, peripheral CD8+ T cell exhaustion, which is defined by poor T cell function linked to increased IR expression (eg: PD1, LAG3, neuropilin-1 [NRP1]). LAG3 is the third IR to be targeted in the clinic, consequently garnering considerable interest and scrutiny. However, persistent antigen exposure in the tumor microenvironment results in sustained PD1/LAG3 expression, contributing to a state of exhaustion manifest in impaired proliferation and cytokine production. This presentation will include a brief introduction LAG3, discussion of the signaling mechanism used by LAG3, and assessment of new insight in to the function of LAG3, alone and in combination with PD1, from mouse models of cancer and analysis of samples from patients treated with LAG3/PD1 therapeutics.
Citation Format: Dario A. Vignali. LAG3: The third checkpoint inhibitor. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr PL04-05.
Abstract SY04-01: Understanding and targeting RNA splicing in breast cancer
Authors: Anczukow Olga A. 
Published: April 2023

Abstract
Upregulation of SR proteins, a family of 14 essential splicing factors (SFs), often occurs in solid tumors, including in breast, and promotes mammary cell transformation and metastasis in breast cancer models. SR protein alterations often occur without genomic copy number changes, suggesting misregulation at the transcriptional or post-transcriptional levels; however the underlying molecular mechanisms are incompletely understood. Here we investigate the transcriptional and post-transcriptional regulatory mechanisms that control SF-levels in normal mammary cells and their misregulation in cancer cells. We demonstrate the MYC oncogene directly regulates a network of splicing factors that are co-expressed in tumors and act together to regulate their downstream targets and promote cell invasion. In parallel, our work reveals the role of non-coding ‘poison exon’ in regulating SR proteins levels during cell differentiation and in tumors. We uncover an extensive cross-regulatory network used by the SR protein family to control their expression. Finally, we develop splice-switching antisense oligonucleotides to reverse the increased skipping of TRA2β poison exon detected in tumors, leading to selective anti-proliferative effects across a variety of cancer models. In summary, our work provides new insights into the regulatory mechanisms of SR proteins and identifies novel oncogenic spliced isoforms that represent potential biomarkers and targets for therapeutics development.
Citation Format: Olga A. Anczukow. Understanding and targeting RNA splicing in breast cancer. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr SY04-01.
Abstract SY17-03: Age-related changes in the tumor microenvironment drive tumor progression
Authors: Stewart Sheila A. 
Published: April 2023

Abstract
Cancer is a disease of the aged. While the accumulation of cell autonomous mutations contributes to tumorigenesis, the central role age-related changes in the tumor microenvironment play in the transformation process is becoming more fully appreciated. Underscoring the importance of an aged microenvironment in cancer development are findings that senescent fibroblasts, which accumulate with age, directly stimulate preneoplastic and neoplastic cell growth and tumor progression. Investigations into how senescent fibroblasts promote tumorigenesis revealed that they express a plethora of growth factors, extracellular matrix remodeling enzymes, chemokines, and cytokines collectively referred to as the senescence associated secretory phenotype (SASP). To identify a role for senescent stromal cells in a spontaneous model of breast cancer, we used single cell RNA-Sequencing (scRNA-Seq) to interrogate the cell types and their functional status in the MMTV-PyMT breast tumor model. This analysis revealed the presence of senescent cancer associated fibroblasts (senCAFs) that we also identified in a human triple negative breast cancer scRNA-Seq database. To establish a role for senCAFs in breast cancer, we mated the PyMT model to the INKATTAC (INK) mouse that allowed us to inducibly eliminate senescent cells. We found that the elimination of senCAFs reduced tumor growth and unleashed the killing power of NK cells. We will discuss the mechanism by which senCAFs impact NK cell killing.
Citation Format: Sheila A. Stewart. Age-related changes in the tumor microenvironment drive tumor progression. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr SY17-03.
Implementasi Model Pembelajaran STEM Tipe Index Card Match Terhadap Keterampilan Berpikir Kritis Pada Materi Alat-Alat Optik
Authors: Nursabrina Fakhira , Laksanawati Wahyu Dian 
Published: April 2023

Penelitian ini bertujuan untuk mengimplentasikan model pembelajaran STEM STEM tipe Index Card Match terhadap keterampilan berpikir kritis pada materi alat-alat optik. Penelitian ini dilaksanakan pada semester genap tahun ajaran 2021-2022. Metode yang digunakan dalam penelitian ini yaitu Pre-Experimental Desain dengan jenis penelitian One-Group Pretest-Posttest Design. Populasi dalam penelitian ini yaitu peserta didik kelas XI MIPA 5 di SMAN 6 Kabupaten Tangerang. Teknik pengambilan sampel dengan menggunakan yaitu Cluster Random Sampling. Data yang dikumpulkan berdasarkan hasil dari Pretest, Posttest, dan aktivitas belajar peserta didik yang disusun berdasarkan indikator berpikir kritis, yaitu: (1) merumuskan masalah; (2) memberikan argument; (3) melakukan deduksi; (4) melakukan induksi; (5) melakukan evaluasi. Kemudian data tersebut dianalisis dengan menggunakan uji-t. Hasil uji hipotesis didapatkan dan . Karena , maka terdapat pengaruh pada model pembelajaran STEM Tipe Index Card Match. Dan juga aktivitas peserta didik mengalami peningkatan selama tiga kali pertemuan. Dapat dikatakan demikian karena setelah melalui proses perhitungan pada pertemuan ke-1 diperoleh persentase pada setiap aspek adalah 45%, 34%, 39%, dan 39%. Pada pertemuan ke-2 diperoleh presentase sebesar 60%, 57%, 51%, dan 56%, pada pertemuan ke-3 diperoleh presentase sebesar 71%, 72%, 75%, dan 78%. Oleh sebab itu, dapat dikatakan bahwa aktivitas peserta didik meningkat. Maka dapat disimpulkan bahwa terdapat peningkatan keterampilan berpikir kritis dengan mengimplementasikan pembelajaran STEM tipe Index Card Match pada materi Alat-Alat Optik.
Microcontroller for Measuring Train Speed as a Learning Resource for Physics Students
Authors: Ermawati Imas Ratna , Martin Martin , Hartini Tri Isti , Arjo Sugianto , Supriyatna Supriyatna , Salsabilla Reza Annisa 
Published: April 2023

The simulation of uniform straight motion using a miniature train based on the Arduino Uno has been carried out to determine velocity through a complex number approach. The experiment was conducted repeatedly at the same distance to obtain a constant velocity. The equation used to determine the velocity of the train is the application of complex number a + ib with the function in variation of power. The results show that the velocity of the trains obtained has the same value, thus proving that the uniform straight motion is valid with the same acceleration which is zero (a=0) in this experiment.
Subjection and Freedom among the Angels
Authors: Schwartz Daniel 
Published: April 2023

Abstract
Medieval theologians commonly held that angels are subordinated one to the other. However, they did not agree on the foundations and nature of this order of subjection. This article traces the trajectory of the theological discussion on the nature of the angelic prelacy. While there is extensive scholarly literature on medieval theologians’ conceptions of the angelic hierarchy, there is next to nothing on their views of angelic prelacy. This article suggests that one of the questions that drives the route taken by the discussions on angelic prelacy is a tension internal to the very idea of angelic prelacy. To be a subject involves some degree of unfreedom. Yet it seemed unsuitable to medieval theologians to attribute unfreedom to angels. A satisfactory picture of angelic prelacy had to posit a form of subjection that did not imply any loss of liberty for the subject angels.
An update and review of commensals on Chilean inland water Decapoda
Authors: De los Ríos-Escalante Patricio R. , Jara-Seguel Pedro , Contreras Angel , Latsague Mirtha , Lara Gladys 
Published: April 2023

Abstract
The commensals on Chilean Decapoda of inland waters are characterized by a marked microendemism. The aim of the present study was to do an exhaustive review on the ectocommensals on Chilean inland-water decapods. The literature revealed the presence of species of Protozoa, viz., eight known only from Parastacus pugnax (Lagenophrys andos, Operculigera insolita, O. parastacis, O. seticola, O. striata, O. taura, Chilenophrys allendei, and Circolagenophrys shiftus), one for both P. pugnax and P. nicoletti (L. anticthos), one for Parastacus pugnax and Samastacus spinifrons (O. asymmetrica), and one reported for Aegla laevis (O. velata). In addition, the presence of flatworms was described, specifically Temnocephala chilensis, associated with A. laevis, P. pugnax, and S. spinifrons, and species of Polychaeta, i.e., Stratiodrilus platensis associated with A. abtao and A. bahamondei, S. aeglaphilus for A. laevis, and S. pugnaxi for P. nicoletti. Biogeographical, ecological and taxonomic implications are discussed.
Paracyclops fiersi sp. nov. (Copepoda, Cyclopoida, Cyclopidae), a new freshwater cyclopoid from north-western Mexico
Authors: Gómez Samuel 
Published: April 2023

Abstract
Several individuals of free-living copepods were found in tap water of the Unidad Académica Mazatlán (Instituto de Ciencias del Mar y Limnología). These copepods turned out to belong to a new species of the freshwater genus Paracyclops. Paracyclops poppei, P. hirsutus, and P. chiltoni were the only species reported in Mexican freshwater ecosystems, and the record of P. fiersi sp. nov. is the fourth confirmed species of the genus from Mexico. The new species seems to be related to P. hardingi and P. bromeliacola. The new species differs in the ornamentation of the female antennary coxobasis, and in the posterior, subdistal, medial spinules on the coxa of the first–fourth leg in both sexes. The city of Mazatlan is supplied with groundwater from an aquifer of the Presidio River (south of Mazatlan), and the new species is most probably distributed in the southern state of Sinaloa (north-western Mexico).
U.S.–Japan Economic Contention in Manchukuo: What did Manchukuo’s Economic Control Bring to the U.S.–Japan Relationship?
Authors: Yukawa Hayato 
Published: April 2023

Abstract
This article examines the impact of Japan’s economic control of Manchukuo on U.S.-Japan relations. From 1933 to 1935, ties between the two countries came to a temporary standstill. However, during these years, Washington and Tokyo waged a diplomatic war in the background over Japan’s control of Manchukuo’s economy. Although the United States accused Japan of violating the Nine Power Treaty it had signed endorsing the Open Door Policy, Japan established several special companies in Manchukuo, and some American firms withdrew from Manchuria. What kind of diplomatic negotiations developed between the United States and Japan during this period? What impact did they have on the relationship between the two countries? This article examines Japan’s development of economic control in Manchukuo and considers its impact, while situating the matter within the history of U.S.-Japan relations during the interwar period. In doing so, it will show how Japan’s Ministry of Foreign Affairs implemented measures that prevented Japan’s economic domination of Manchukuo from immediately worsening U.S.-Japan relations. At the same time, it demonstrates that Japan’s Ministry of Foreign Affairs, as well as its army, played an important role in the process of Japan asserting dominance over Manchuria.