bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective single-center study of health-related quality of life after COVID-19 in ICU and non-ICU patients
Authors: Herrmann Johannes , Müller Kerstin , Notz Quirin , Hübsch Martha , Haas Kirsten , Horn Anna , Schmidt Julia , Heuschmann Peter , Maschmann Jens , Frosch Matthias , Deckert Jürgen , Einsele Hermann , Ertl Georg , Frantz Stefan , Meybohm Patrick , Lotz Christopher 
Published: April 2023

AbstractLong-term sequelae in hospitalized Coronavirus Disease 2019 (COVID-19) patients may result in limited quality of life. The current study aimed to determine health-related quality of life (HRQoL) after COVID-19 hospitalization in non-intensive care unit (ICU) and ICU patients. This is a single-center study at the University Hospital of Wuerzburg, Germany. Patients eligible were hospitalized with COVID-19 between March 2020 and December 2020. Patients were interviewed 3 and 12 months after hospital discharge. Questionnaires included the European Quality of Life 5 Dimensions 5 Level (EQ-5D-5L), patient health questionnaire-9 (PHQ-9), the generalized anxiety disorder 7 scale (GAD-7), FACIT fatigue scale, perceived stress scale (PSS-10) and posttraumatic symptom scale 10 (PTSS-10). 85 patients were included in the study. The EQ5D-5L-Index significantly differed between non-ICU (0.78 ± 0.33 and 0.84 ± 0.23) and ICU (0.71 ± 0.27; 0.74 ± 0.2) patients after 3- and 12-months. Of non-ICU 87% and 80% of ICU survivors lived at home without support after 12 months. One-third of ICU and half of the non-ICU patients returned to work. A higher percentage of ICU patients was limited in their activities of daily living compared to non-ICU patients. Depression and fatigue were present in one fifth of the ICU patients. Stress levels remained high with only 24% of non-ICU and 3% of ICU patients (p = 0.0186) having low perceived stress. Posttraumatic symptoms were present in 5% of non-ICU and 10% of ICU patients. HRQoL is limited in COVID-19 ICU patients 3- and 12-months post COVID-19 hospitalization, with significantly less improvement at 12-months compared to non-ICU patients. Mental disorders were common highlighting the complexity of post-COVID-19 symptoms as well as the necessity to educate patients and primary care providers about monitoring mental well-being post COVID-19.
Rapid adsorptive removal of chromium from wastewater using walnut-derived biosorbents
Authors: Garg Rajni , Garg Rishav , Sillanpää Mika , Alimuddin  , Khan Mohammad Amir , Mubarak Nabisab Mujawar , Tan Yie Hua 
Published: April 2023

AbstractContamination of water resources by industrial effluents containing heavy metal ions and management of solid waste from agricultural and food industries is a serious issue. This study presents the valorization of waste walnut shells as an effective and environment-friendly biosorbent for sequestrating Cr(VI) from aqueous media. The native walnut shell powder (NWP) was chemically modified with alkali (AWP) and citric acid (CWP) to obtain modified biosorbents with abundant availability of pores as active centers, as confirmed by BET analysis. During batch adsorption studies, the process parameters for Cr(VI) adsorption were optimized at pH 2.0. The adsorption data were fitted to isotherm and kinetic models to compute various adsorption parameters. The adsorption pattern of Cr(VI) was well explained by the Langmuir model suggesting the adsorbate monolayer formation on the surface of the biosorbents. The maximum adsorption capacity, qm, for Cr(VI) was achieved for CWP (75.26 mg/g), followed by AWP (69.56 mg/g) and NWP (64.82 mg/g). Treatment with sodium hydroxide and citric acid improved the adsorption efficiency of the biosorbent by 4.5 and 8.2%, respectively. The endothermic and spontaneous adsorption was observed to trail the pseudo-second-order kinetics under optimized process parameters. Thus, the chemically modified walnut shell powder can be an eco-friendly adsorbent for Cr(VI) from aqueous solutions.
Scalable continuous-flow electroporation platform enabling T cell transfection for cellular therapy manufacturing
Authors: VanderBurgh Jacob A. , Corso Thomas N. , Levy Stephen L. , Craighead Harold G. 
Published: April 2023

AbstractViral vectors represent a bottleneck in the manufacturing of cellular therapies. Electroporation has emerged as an approach for non-viral transfection of primary cells, but standard cuvette-based approaches suffer from low throughput, difficult optimization, and incompatibility with large-scale cell manufacturing. Here, we present a novel electroporation platform capable of rapid and reproducible electroporation that can efficiently transfect small volumes of cells for research and process optimization and scale to volumes required for applications in cellular therapy. We demonstrate delivery of plasmid DNA and mRNA to primary human T cells with high efficiency and viability, such as > 95% transfection efficiency for mRNA delivery with < 2% loss of cell viability compared to control cells. We present methods for scaling delivery that achieve an experimental throughput of 256 million cells/min. Finally, we demonstrate a therapeutically relevant modification of primary T cells using CRISPR/Cas9 to knockdown T cell receptor (TCR) expression. This study displays the capabilities of our system to address unmet needs for efficient, non-viral engineering of T cells for cell manufacturing.
Expansion of mixed immune cells using CD3/CD161 co-stimulation for the treatment of cancer
Authors: Tsumura Ryo , Haruta Miwa , Kuwano Masataka , Yasunaga Masahiro 
Published: April 2023

AbstractAdoptive cell transfer (ACT) is a type of personalized immunotherapy in which expanded immune cells are administered to patients with cancer. However, single-cell populations, such as killer T cells, dendritic cells, natural killer (NK) cells, and NKT (NKT) cells, have been generally used, and their effectiveness remains limited. Here, we established a novel culture method via CD3/CD161 co-stimulation and successfully expanded CD3+/CD4+ helper T cells, CD3+/CD8+ cytotoxic T cells (CTLs), CD3−/CD56+ NK cells, CD3+/CD1d+ NKT cells, CD3+/CD56+ NKT cells, CD3+/TCRγδ+ T cells, and CD3−/CD11c+/HLA-DR+ dendritic cells in peripheral blood mononuclear cells from healthy donors; their respective numbers were 155.5, 1132.5, 5.7, 117.0, 659.2, 325.6, and 6.8 times higher than those before expansion. These mixed immune cells showed strong cytotoxicity against cancer cell lines Capan-1 and SW480. Moreover, both CD3+/CD8+ CTLs and CD3+/CD56+ NKT cells killed tumor cells in cell contact-dependent and -independent manners via granzyme B and interferon-γ/TNF-α, respectively. Furthermore, the cytotoxicity of the mixed cells was significantly superior to that of CTLs or NKTs alone. A bet-hedging CTL-NKT circuitry is one potential mechanism underlying this cooperative cytotoxicity. Collectively, CD3/CD161 co-stimulation may be a promising culture method to expand multiple, distinct immune cell populations for the treatment of cancer.
Monitoring genotoxic, biochemical and morphotoxic potential of penoxsulam and the protective role of European blueberry (Vaccinium myrtillus L.) extract
Authors: Acar Ali , Singh Divya 
Published: April 2023

AbstractThe present study aimed at exploring to explore the penoxsulam toxicity and protective effects of blueberry extract in roots of Allium cepa L. The effective concentration (EC50) of penoxsulam was determined at 20 µg/L by the root growth inhibition test as the concentration reducing the root length by 50%. The bulbs of A. cepa L. were treated with tap water, blueberry extracts (25 and 50 mg/L), penoxsulam (20 µg/L) and combination of blueberry extracts (25 and 50 mg/L) with penoxsulam (20 µg/L) for 96 h. The results revealed that penoxsulam exposure inhibited cell division, rooting percentage, growth rate, root length and weight gain in the roots of A. cepa L. In addition, it induced chromosomal anomalies such as sticky chromosome, fragment, unequal distribution of chromatin, bridge, vagrant chromosome and c-mitosis and DNA strand breaks. Further, penoxsulam treatment enhanced malondialdehyde content and SOD, CAT and GR antioxidant enzyme activities. Molecular docking results supported the up-regulation of antioxidant enzyme SOD, CAT and GR. Against all these toxicity, blueberry extracts reduced penoxsulam toxicity in a concentration-dependent manner. The highest amount of recovery for cytological, morphological and oxidative stress parameters was observed when using blueberry extract at a concentration of 50 mg/L. In addition, blueberry extracts application showed a positive correlation with weight gain, root length, mitotic index and rooting percentage whereas a negative correlation with micronucleus formation, DNA damage, chromosomal aberrations, antioxidant enzymes activities and lipid peroxidation indicating its protecting effects. As a result, it has been seen that the blueberry extract can tolerate all these toxic effects of penoxsulam depending on the concentration, and it has been understood that it is a good protective natural product against such chemical exposures.
A systematic review of neurophysiological sensing for the assessment of acute pain
Authors: Fernandez Rojas Raul , Brown Nicholas , Waddington Gordon , Goecke Roland 
Published: April 2023

AbstractPain is a complex and personal experience that presents diverse measurement challenges. Different sensing technologies can be used as a surrogate measure of pain to overcome these challenges. The objective of this review is to summarise and synthesise the published literature to: (a) identify relevant non-invasive physiological sensing technologies that can be used for the assessment of human pain, (b) describe the analytical tools used in artificial intelligence (AI) to decode pain data collected from sensing technologies, and (c) describe the main implications in the application of these technologies. A literature search was conducted in July 2022 to query PubMed, Web of Sciences, and Scopus. Papers published between January 2013 and July 2022 are considered. Forty-eight studies are included in this literature review. Two main sensing technologies (neurological and physiological) are identified in the literature. The sensing technologies and their modality (unimodal or multimodal) are presented. The literature provided numerous examples of how different analytical tools in AI have been applied to decode pain. This review identifies different non-invasive sensing technologies, their analytical tools, and the implications for their use. There are significant opportunities to leverage multimodal sensing and deep learning to improve accuracy of pain monitoring systems. This review also identifies the need for analyses and datasets that explore the inclusion of neural and physiological information together. Finally, challenges and opportunities for designing better systems for pain assessment are also presented.
Assessing nocturnal scratch with actigraphy in atopic dermatitis patients
Authors: Ji Ju , Venderley Jordan , Zhang Hui , Lei Mengjue , Ruan Guangchen , Patel Neel , Chung Yu-Min , Giesting Regan , Miller Leah 
Published: April 2023

AbstractNocturnal scratch is one major factor leading to impaired quality of life in atopic dermatitis (AD) patients. Therefore, objectively quantifying nocturnal scratch events aids in assessing the disease state, treatment effect, and AD patients’ quality of life. In this paper, we describe the use of actigraphy, highly predictive topological features, and a model-ensembling approach to develop an assessment of nocturnal scratch events by measuring scratch duration and intensity. Our assessment is tested in a clinical setting against the ground truth obtained from video recordings. The new approach addresses unmet challenges in existing studies, such as the lack of generalizability to real-world applications, the failure to capture finger scratches, and the limitations in the evaluation due to imbalanced data in the current literature. Furthermore, the performance evaluation shows agreement between derived digital endpoints and the video annotation ground truth, as well as patient-reported outcomes, which demonstrated the validity of the new assessment of nocturnal scratch.
A Complexity Approach to Promoting Within- and Cross-Language Generalization in Bilingual Children With Phonological Delays
Authors: Irizarry-Pérez Carlos D. , Fabiano-Smith Leah , Martinez-Fisher Andrea 
Published: April 2023

Purpose:
The purpose of this study was to explore the effects of generalization when treating complex targets with shared sounds in Spanish for a 5-year-old Spanish–English bilingual child with a phonological delay.

Method:
Two complex clusters (/fl/ and /fɾ/) and one additional target (/l/) were chosen for treatment. Intervention sessions were held in Spanish over the course of 1 year on a weekly basis. The accuracy of the treated and untreated targets was monitored using a single-subject case design and was assessed using visual analysis.

Results:
The accuracy of the production of treated targets increased upon administering the intervention. Accuracy also increased for untreated /fl/ targets in Spanish and English, /l/ in English, and untreated /fɾ/ clusters in Spanish.

Conclusions:
The results suggest that choosing complex targets consisting of shared sounds helps promote the generalization of skills within and across languages. Future studies should examine the outcomes of selecting additional forms of complex targets for bilingual children.
A sequential acid-base mechanism in the interstellar medium: The emergence of cis-formic acid in dark molecular clouds
Authors: García de la Concepción J. , Jiménez-Serra I. , Corchado J. C. , Molpeceres G. , Martínez-Henares A. , Rivilla V. M. , Colzi L. , Martín-Pintado J. 
Published: April 2023

Context. The different abundance ratios between isomers of an organic molecule observed in the interstellar medium (ISM) provide valuable information about the chemistry and physics of the gas and the history of molecular clouds. In this context, the origin of an abundance of cis-formic acid (c-HCOOH) of only 6% the trans isomer (t-HCOOH) abundance in cold cores remains unknown.
Aims. In this work, we aim to explain the presence of c-HCOOH in dark molecular clouds through the destruction and back formation of c-HCOOH and t-HCOOH in a cyclic process that involves HCOOH and highly abundant molecules such as HCO+ and NH3.
Methods. We used high-level ab initio methods to compute the potential energy profiles for the cyclic destruction and formation routes of c-HCOOH and t-HCOOH. Accurate global rate constants and branching ratios are calculated based on the transition state theory and the master equation formalism under the typical conditions of the ISM.
Results. The destruction of HCOOH by reaction with HCO+ in the gas phase leads to three isomers of the cation HC(OH)2+. The most abundant cation can react in a second step with other abundant molecules of the ISM such as NH3 to form back c-HCOOH and t-HCOOH. This mechanism explains the formation of c-HCOOH in dark molecular clouds. Considering this mechanism, the fraction of c-HCOOH with respect t-HCOOH is 25.7%. To explain the 6% reported by the observations, we propose that further destruction mechanisms of the cations of HCOOH by collisions with abundant molecules or interconversion reactions on dust grains should be taken into account.
Conclusions. The sequential acid-base (SAB) mechanism proposed in this work involves fast processes with very abundant molecules in the ISM. Thus, HCOOH very likely suffers our proposed transformations in the conditions of dark molecular clouds such as B5 and L483. This is a new approach in the framework of the isomerism of organic molecules in the ISM, which has the potential to explain the ratio between isomers of organic molecules detected in the ISM.
Von einer enterovesikalen Fistel zum Mammakarzinom
Authors: Comploj Evi , Hanspeter Esther , Bonatti Matteo , Palermo Salvatore , Mazzucato Giovanni , Pycha Armin , Trenti Emanuela 
Published: April 2023

ZusammenfassungWir berichten über den Fall einer 83-jährigen Patientin, die unter dem Verdacht einer enterovesikalen Fistel hospitalisiert und operiert wurde und schlussendlich die Diagnose eines intestinal metastasierten Mammakarzinoms erhielt.
Vaginal Progesterone Is Associated with Intrahepatic Cholestasis of Pregnancy
Authors: Tsur Abraham , Leonard Stephanie A. , Kan Peiyi , Datoc Imee A. , Girsen Anna I. , Shaw Gary M. , Stevenson David K. , El-Sayed Yasser Y. , Druzin Maurice L. , Blumenfeld Yair J. 
Published: April 2023

Objective The frequency of intrahepatic cholestasis of pregnancy (ICP) peaks during the third trimester of pregnancy when plasma progesterone levels are the highest. Furthermore, twin pregnancies are characterized by higher progesterone levels than singletons and have a higher frequency of cholestasis. Therefore, we hypothesized that exogenous progestogens administered for reducing the risk of spontaneous preterm birth may increase the risk of cholestasis. Utilizing the large IBM MarketScan Commercial Claims and Encounters Database, we investigated the frequency of cholestasis in patients treated with vaginal progesterone or intramuscular 17α-hydroxyprogesterone caproate for the prevention of preterm birth.
Study Design We identified 1,776,092 live-born singleton pregnancies between 2010 and 2014. We confirmed second and third trimester administration of progestogens by cross-referencing the dates of progesterone prescriptions with the dates of scheduled pregnancy events such as nuchal translucency scan, fetal anatomy scan, glucose challenge test, and Tdap vaccination. We excluded pregnancies with missing data regarding timing of scheduled pregnancy events or progesterone treatment prescribed only during the first trimester. Cholestasis of pregnancy was identified based on prescriptions for ursodeoxycholic acid. We used multivariable logistic regression to estimate adjusted (for maternal age) odds ratios for cholestasis in patients treated with vaginal progesterone, and in patients treated with 17α-hydroxyprogesterone caproate compared with those not treated with any type of progestogen (the reference group).
Results The final cohort consisted of 870,599 pregnancies. Among patients treated with vaginal progesterone during the second and third trimester, the frequency of cholestasis was significantly higher than the reference group (0.75 vs. 0.23%, adjusted odds ratio [aOR]: 3.16, 95% confidence interval [CI]: 2.23–4.49). In contrast, there was no significant association between 17α-hydroxyprogesterone caproate and cholestasis (0.27%, aOR: 1.12, 95% CI: 0.58–2.16)
Conclusion Using a robust dataset, we observed that vaginal progesterone but not intramuscular 17α-hydroxyprogesterone caproate was associated with an increased risk for ICP.
Key Points
Alteration of Lipid Profile Between Subclinical Hypothyroidism and
Well-Matched Controls: A Meta-Analysis
Authors: Liu Jinjin , Chen Yun , Ren Bingxuan , He Yanhong , Li Fan , Wang Lingbo , Wan Siyuan , Liu Lixiang , Shen Hongmei 
Published: April 2023

AbstractIn previous studies, subclinical hypothyroidism (SCH) has been associated with
altered lipid profiles. However, since the discrepancy between these study
results may reside in the great heterogeneity of the populations studied, this
relationship is controversial. This study aimed to explore the changes in total
cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol
(LDL-c), and high-density lipoprotein cholesterol (HDL-c) between subclinical
hypothyroidism (SCH) and well-matched euthyroid (EU) groups. Multiple databases
were searched for publications before December 1, 2021, including
cross-sectional studies on the association between SCH and lipid profile matched
by age, gender, and BMI. Twenty-five articles with 3347 participants were
included for meta-analysis. The results showed that the TC, TG, and LDL-c levels
of the SCH groups were higher than the EU groups (TC, SMD=0.49,
95% CI 0.27, 0.71, p<0.001) (TG, SMD=0.43, 95%
CI 0.21, 0.64, p<0.05 ) (LDL-c, SMD=0.75, 95% CI 0.46,
1.03, p<0.001 ). The HDL-c levels of the SCH group were lower than the
control group (SMD=–0.53, 95% CI –0.81,
–0.25, p<0.05). SCH has a larger impact on LDL-c than the other
three indicators. After subgroup analyses, there was a larger impact on lipid
alteration in the subgroup of TSH>10 μIU/ml, especially
on LDL-c. This study found that SCH was associated with altered lipid profiles.
Appropriate clinical treatment may be needed to prevent dyslipidemia and related
diseases.
Borylative Difunctionalization of Unsaturated Hydrocarbons Using Borylcopper Species
Authors: Fujihara Tetsuaki 
Published: April 2023

AbstractThis account summarizes the Cu-catalyzed borylative functionalizations of unsaturated hydrocarbons that we have developed over the past decade. First, we focus on the regioselective hydroboration of unsymmetrical internal alkynes and 1,2-dienes. Secondly, a borylative allyl–allyl coupling using 1,2-dienes, B2pin2, and an allyl phosphate is presented. Thirdly, we introduce the boroacylation and boroformylation of 1,2-dienes by using an acid anhydride or formate, respectively, as the electrophile. Lastly, we describe the synthesis of 2-boryl-1,3-butadienes and cyclic allylborates. These reactions offer a versatile method for synthesizing a broad range of useful compounds and also provide a detailed understanding of the reaction mechanism, which could lead to the development of catalysts that are both more efficient and more selective.1	Introduction2	Hydroboration of Alkynes3	Hydroboration of 1,2-Dienes and 1,3-Dienes4	Synthesis of 2-Boryl-1,3-butadienes5	Boroallylation of 1,2-Dienes6	Boroacylation of 1,2-Dienes7	Boroformylation of 1,2-Dienes and 1,3-Dienes8	Synthesis of Cyclic Allylborates9	Conclusion and Outlook
Novel Zinc(II) Phthalocyanine Dyes for Color Photoresists
Authors: Chen Pengzhong , Wang Wanying , Yao Qichao , Xu Runfeng , Wang Ankang , Peng Xiaojun 
Published: April 2023

AbstractColor photoresists are the key materials for the fabrication of color filters (CFs). Organic dyes offer a promising alternative to the conventional pigment-based system to make CFs with higher resolution. However, the stability of dye molecules is an urgent problem to be solved. Herein, we designed and synthesized a series of highly stable zinc phthalocyanine dyes containing polymerizable acrylamide groups. Upon light exposure, dense and insoluble network structures were formed in the prepared CFs, which increase the thermal stability and anti-migration capacity of these dyes.
Electrochemical Cyclization of Hydrazones and Amidines To Access Trisubstituted 1,2,4-Triazoles
Authors: Tang Dong , Jiang Rui , Mu Yangxiu , Hou Jing , Wan Yaya , Hong Yu , Yang Zhixiang 
Published: April 2023

AbstractA KI-mediated electrochemical method has been developed for the synthesis of trisubstituted 1,2,4-triazoles from easily accessible hydrazones and amidines derivatives. The protocol provides various aryl and alkyl 1,2,4-triazoles in moderate to good yields with good functional-group tolerance and without the need for any chemical oxidants in an undivided cell.
Solvent-Free Tandem Reaction of P(O)–OH and an Epichlorohydrocarbon for Controllable O-Alkylation through a Ring-Opening and Annulation Process
Authors: Yang Jia , Xu Qian , Luo Yi , Huang Yirui , Wang Zixuan , Chen Wenyu , Zhu Liang , Yuan Huihui , Wang Shuai , Zhong Hong 
Published: April 2023

AbstractWe report an efficient additive- and solvent-free O-alkylation of P(O)–OH compounds with epichlorohydrocarbons for the synthesis of epoxy-group-containing phosphinates. The transformation proceeds through a tandem reaction involving ring-opening and annulation processes. The ring-opened products and dechlorinated products can be obtained selectively by controlling the reaction conditions. This protocol is expected to be useful in industry because it is readily scaled up to a gram level and involves simple posttreatment.