bluuebunny/crossref_metadata_2025_split huggingface dataset:
Organic Geochemistry of Carbonate Rocks, Silicites and Black Shales of the Vendian (Ediacaran) Khatyspyt Formation, Northeastern Siberia
Authors: Melnik D.S. , Parfenova T.M. , Grazhdankin D.V. , Rogov V.I. 
Published: April 2023

Abstract
—The paper presents the results of the study of organic geochemistry of carbonate rocks, silicites, and calcareous mudstones of the Vendian (Ediacaran) Khatyspyt Formation in the northeast of the Siberian Platform. The distribution of dispersed organic matter (OM) is shown to be uneven. The formation comprises carbonate-dominated intervals depleted in OM, with total organic carbon content (TOC) &lt; 0.1%, or weakly enriched in OM (TOC up to 0.4%), interstratified with carbonate-shale, carbonate-shale-siliceous, carbonate-siliceous and siliceous intervals enriched in OM (TOC up to 1–4%). There is also a thin-layered calcareous mudstones anomalously enriched in OM (black shales with TOC at 10%) cropping out in the Khorbusuonka River valley (unit thickness 10 cm). The redox conditions in the water column varied during deposition of the Khatyspyt Formation. Certain intervals characterize a stratified water column and euxinic bottom water conditions. During the study, the assessment was conducted of the OM catagenesis and the generative potential of the Khatyspyt Formation. Based on the results of geochemical study and the synthesis of information on the geological structure of sedimentary succession in the northeastern Siberian Platform, the Khatyspyt Formation has been evaluated as one of the hydrocarbon sources for the territory.
A study on digital twin technology of Human-machine integration cross-belt Sorting System
Authors: Qu Yanbo , Zhao Ning , Zhang Haojue 
Published: April 2023

Abstract
So far nearly 110 billion pieces of express items have been handled by Chinese express delivery industry, with an average annual growth of 200%. Cross-belt sorting system serves as the most important express item sorting system and most of the sorting work has been automated fulfilled. However, operators are still needed and working pressure arisen with the growth of express items. Under the context of the industry 5.0, new technologies should be utilized to enhance both welfare of the workers and working efficiency of the cross-belt system. However, it’s hard to strike a good balance between express item handling efficiency and the happiness of operators. Digital twin technology bring new solution to this problem. Based on the ascertained human-machine features of the distribution center, the human-machine integration digital twin frame, consisting of physical space, virtual space, twin data center and supporting & application services, were proposed. The closed-loop interactions among biological human body, virtual human body, virtual equipment and physical equipment were achieved. The express item distribution center operation system was established based on the digital twin technologies. Besides, the physical-level data collection and storage, the human body fatigue efficiency conversion process on services levels and the virtual-level predication & decision-making were elaborated. Finally, methods to establish the cross-belt sorting system for the express item distribution center based on digital twin technologies were proposed. Feasibility of the aforesaid methods was verified by a simulation case study.
Chlorpyrifos in Fish and Seals from the Canadian Arctic
Authors: Muir Derek , Evans Marlene , Gamberg Mary , Houde Magali , Kirk Jane , Stern Gary , Ross Tyler , Thiemann Gregory , Morris Adam 
Published: April 2023

Abstract
Chlorpyrifos (CPY), a widely used organothiophosphate insecticide, has been proposed for listing on Annex A of the Stockholm Convention on Persistent Organic Pollutants. While CPY has been widely measured in Arctic air and seawater, information on bioaccumulation of CPY in aquatic food webs in the Arctic is very limited. This study presents results of additional monitoring data on CPY in fish and marine mammal samples from the Canadian Arctic/sub-Arctic based on ongoing studies under Canada’s Northern Contaminants Program. The majority of the CPY data was from samples collected from 2011 to 2021 and analysed using USEPA Method 1699, involving quantification by high resolution mass spectrometry. The available dataset showed low detection frequencies of CPY ranging from zero in arctic grayling (Thymallus arcticus) to 52% in arctic cod (Boreogadus saida). To enable statistical analysis the non-detects were substituted with ½ the detection limit (½ DL) based on the assumption that the non-detects were not zero and previous studies showing CPY was consistently detected in water and air in the Canadian Arctic. Largest geometric mean CPY concentrations were found in lake whitefish (Coregonus clupeaformis) muscle (0.56 ng/g wet wt) while burbot (Lota lota) liver had the highest maximum concentration (8.2 ng/g). Log10 CPY concentrations (with ½ DL substitution) were significantly correlated with % lipid and length in lake trout (Salvelinus namaycush) muscle and with % lipid in burbot liver. Geomean concentrations in ringed seal (Pusa hispida) blubber from 8 communities ranged from 0.005 to 0.605 ng/g and showed no geo-spatial trends. Overall, the results indicate widespread but very low level contamination of Arctic fish and seals by CPY.
Therapeutic effects of the stromal vascular fraction and adipose-derived stem cells on osteoarthritis in an experimental rabbit model
Authors: Anjiki Kensuke , Matsumoto Tomoyuki , Kuroda Yuichi , Fujita Masahiro , Hayashi Shinya , Nakano Naoki , Maeda Toshihisa , Takashima Yoshinori , Kikuchi Kenichi , Ikuta Kenmei , Onoi Yuma , Tachibana Shotaro , Suda Yoshihito , Wada Kensuke , Matsushita Takehiko , Kuroda Ryosuke 
Published: April 2023

Abstract
Background The stromal-vascular fraction (SVF), comprising heterogeneous cell populations and adipose-derived stem cells (ADSCs), has therapeutic potential against osteoarthritis (OA); however, the underlying mechanism remains elusive. This study investigated the therapeutic effects of the heterogeneous cells in rabbit SVF on rabbit chondrocytes and compared them with those of two different ADSCs adjusted for their stem cell content.Methods Animals were divided into control, low-dose ADSCs (lADSC; 1×104 ADSCs), high-dose ADSCs (hADSC; 1×105 ADSCs), and SVF (1×105 SVF) groups. SVF and ADSCs isolated from rabbits were autografted into the knees after OA onset, and the animals were sacrificed 8 and 12 weeks after OA onset. OA progression and synovitis was analyzed by macroscopic and histological analyses, and the expression of collagen type II, SOX9, MMP-13, CD86 (M1) and CD163 (M2) was assessed using immunohistochemical and real-time polymerase chain reaction (RT-PCR). In vitro, chondrocytes were either co-cultured with SVF or lADSCs or alone (control), and the pellet size was evaluated. TGF-β and IL-10 concentrations in the medium were evaluated using an enzyme-linked immunosorbent assay. Additionally, immune-fluorescent double staining was performed for SVF and lADSCs to stain macrophages with F4/80, CD86, and CD163.Results OA progression was significantly suppressed, and synovitis was reduced in the SVF and hADSC groups. Compared to those in the control and lADSC groups, immunohistochemistry and RT-PCR revealed higher expression of collagen type Ⅱ and SOX9, lower expression of MMP-13 in cartilage, lower macrophage M1/M2 ratio, and higher expression of TGF-β, IL-10 in the synovium in the SVF and the hADSC groups. In the SVF group, the pellet size and TGF-β and IL-10 concentrations were significantly higher than those in the control and lADSC groups. Immune-fluorescent double staining revealed a significantly higher number of M2 than M1 macrophages in SVF.Conclusions The therapeutic effects of SVF and hADSCs superior on chondrocytes was superior with enhanced anabolic and inhibited catabolic factors than those of controls and lADSCs. Heterogeneous cells, mainly M2 macrophages in SVF, contributed to the therapeutic effect on chondrocytes and knee joint homeostasis by enhancing the growth factors and chondrocyte protective cytokines secretion.
MCDA-CRISPR-Cas12-based diagnostic platform for detection of Mycoplasma Pneumoniae Targeting the CARDS gene
Authors: Yang Tingyu , Xiao Jing , Li Feina , Wang Yonghong , Ma Qi , Chen Yuying , Wang Yi , Li Jieqiong 
Published: April 2023

Abstract
Mycoplasma pneumoniae (MP) is a leading cause of pediatric community-acquired pneumonia (CAP) and advanced techniques for Mycoplasma pneumoniae pneumonia (MPP) diagnosis are urgently needed. Here, a novel diagnostic test combined multiple cross displacement amplification (MCDA) with CRISPR-Cas12a system targeting the CARDS gene of MP, termed MP-MCC, were developed for MPP detection. The MCDA assay was employed for nucleic acid amplification, and the CRISPR-Cas12a/CrRNA complex was used to decode the amplification products. Then, the detection result was observed via real-time fluorescence. The optimal conditions for our assay include a MCDA reaction at 61°C for 40 min and a CRISPR detection at 37°C for ~ 5 min. The results showed that reaction products were detectable from as little as 10fg of pure MP templates and from approximately 10 copies of plasmids containing CARDS genes. The specificity in detecting MP strains was 100%, and there was no cross-reaction to non-MP strains. Furthermore, our assay was further validated using clinical samples, which offered high sensitivity and specificity for MPP diagnostic. Taken together, the detection technique developed here has advantages on rapidity, sensitivity and specificity for MP detection, which could be used as a potential tool for clinical MPP diagnosis.
The nurses' perceptions of the obligatory continuing professional development program in a large tertiary hospital
Authors: Zaitoun Rasha Abu , Battat Maher , sakleh Israa , Zabin Loai , ihudiebube-splendor Chikaodili 
Published: April 2023

Abstract
Background
A crucial strategy for preserving skills and job motivation is continuing professional development. This research aims to evaluate how nurses at NNUH perceived the hospital's mandatory continuing professional development (CPD) program.
Method
The study was carried out in a tertiary hospital in Nablus, Palestine. A cross-sectional quantitative descriptive study was conducted from July to September 202 for 202 full-time and in-duty nurses at NNUH using the Chikaodili Ihudiebube-splendor questionnaire.
Results
The Web survey of 202 nurses shows that the nurse’s perception of mandatory continuing education for professional development is statistically significant with age, sex and marital status. The highest mean score was 3.29 for "Continuing education should be made mandatory for nurses," and 2.39 for that mandatory CPD programs are useful for nurses. However, the overall perceived relevance of these items was not positive (2.39 ± 0.77). The mean score for all items related to the reason for participation was greater than 2.5 and the participants had a significant positive score (3.19 ± 0.49). The remaining perceived obstacles to requiring ongoing education for professional growth were greater than 2.5, and the total perceived obstacle was positive (2.80 ± 0.66). Only one item, "Lack of employer's cooperation," fell below the cut-off point of 2.25.
Conclusions
Nurses mostly perceive that continuing education should be made mandatory for nurses, and the things I learn in mandatory continuing education programs will be useful to me. There are many reasons for participating in required education programs, and finally, nurses face many barriers to required continuing education for professional development at NNUH.
Implications
Special attention and targeted support should be provided to promote the continuing professional development of nursing staff. Nurses maintained a positive attitude towards continued professional development, but faced challenges.
Assessment of Multi-Perspective and Multi-Sensor Data for Urban Strata Mapping
Authors: Ramlal Bheshem , Archibald Daniel , Al-Tahir Raid , Sutherland Michael , Davis Dexter 
Published: April 2023

Abstract
Many countries have seen a significant increase in the number of high-rise and multi-story buildings with strata units with the shrinking availability of horizontal land space in urban areas. Most existing cadastres record tenure and associated information in a two-dimensional format, and therefore will need to be upgraded to 3D cadastres to facilitate the recording of title information for these strata units. The geospatial aspects of implementing a 3D cadastre require cost-effective, rapid, accessible, non-labor intensive, and accurate means by which complete data may be collected to model strata units. Existing data sources such as Building Information Models (BIMs) have been used to delineate these units. However, BIMs are not easily accessible in developing countries. There is no single spatial data source that can easily fulfill all the criteria of a 3D data source for mapping urban strata boundaries. However, leveraging multi-perspective and multi-sensor data can theoretically serve this purpose. In this paper, we report on research conducted to demonstrate the feasibility of using low cost and accessible cell phone data, integrated with higher accuracy terrestrial laser scan data, and drone aerial imagery to produce a multi-perspective 3D data source. It was found that the integration of these data sets as a 3D data source, resulted in absolute accuracies within centimetres and decimetres in the horizontal plane and within millimetres in the vertical plane. Overall, the integration of these three sources of data may be appropriate in meeting the needs for a low-cost source of 3D data.
Health-related internet use among outpatient oncology patients and the impact of the Covid-19 pandemic
Authors: Seynstahl Sarah , Erickson Nicole , Jost Nicole , Wuerstlein Rachel , Fey Theres , Nasseh Daniel , Casuscelli Jozefina , Theurich Sebastian , Heinemann Volker 
Published: April 2023

Abstract
Objective The aim of this study was to assess eHealth usage among oncology patients undergoing infusion therapy. Methods We used an extended version of the validated 21-item questionnaire “Use of information and communication technologies questionnaire (ICT)” developed by Seifert et al., in order to assess patient-reported information related to general internet abilities including health-related applications.
Results 303 patients participated. 299/303 participants reported regular internet usage. 72.6% (217/299) use the internet to search for health-related information and 79.1% (235/297) expressed readiness to communicate digitally with health care providers. Decreasing age and higher internet literacy correlated with a more frequent use of eHealth applications (p<0.001). Users of IOS tended to use therapy apps more frequently compared to users of other operating systems (p=0.034). 25% (68/275) reported increased internet usage during the pandemic.
Conclusion The majority of patients were regular internet users and expressed an openness to eHealth. Factors such as internet literacy and average age are important to consider when implementing new eHealth applications. Despite the positive influence of the pandemic on internet usage, there remains a gap between self-reported readiness and real use of eHealth applications.
Practice Implications eHealth applications should be introduced slowly into clinical settings with provided help.
eHealth applications should be introduced slowly into clinical settings regular support is provided
It is important to carefully consider the population’s characteristics to ensure successful implementation of eHealth applications
The pandemic is a driver for the use of the Internet including eHealth
Research on Carbon Allowance Allocation for Public Buildings in China Based on the Target of Carbon Emission Intensity Reduction in 2030
Authors: Zhang Luqi , Liu Yisheng , Du Zhuoqun 
Published: April 2023

Abstract
Due to the increasing energy crisis and climate warming, carbon emission reduction has attracted the attention of all countries. Carbon trading is an effective way to reduce carbon emissions. As one of the three major carbon emitters, the construction sector urgently needs to establish and improve the construction carbon trading market. Since the allocation of carbon emission rights is the first step to carbon trading, it is necessary to reasonably allocate carbon emissions to each province. This paper establishes a set of carbon emission allocation methods based on the principles of equity, efficiency, historical responsibility, emission reduction capacity and environmental level according to the relative emission reduction action target of 2030 proposed by the Chinese government. Correspondingly, urban population, carbon emission efficiency calculated by DEA model, historical cumulative CO2 emissions, ratio of R&D expenditure to GDP and forest coverage are selected as indicators and the entropy weight method is applied to allocate the amount of CO2. Taking the achievement of China's carbon peak target as an example, carbon emission quotas for public buildings in 2030 were calculated for each province. After verification of Gini coefficient and contribution coefficient, the allocation scheme is derived to be compatible with the economy, population and environment. The results show that the CO2 allocation shows a trend of gradual decrease from southeast to northwest, with Guangdong and Jiangsu having the highest carbon quotas and Qinghai and Xinjiang having the lowest. 33.3% of the provinces are under pressure to reduce emissions, with Xinjiang having the highest pressure to reduce emissions, and economically developed regions like Beijing, Zhejiang and Guangdong still face some pressure to reduce emissions. Finally, the allocation method proposed in this paper can be used as a reference for the building sector, and the allocation scheme can help policy makers in each province to formulate an effective and differentiated emission reduction strategy that ensure the successful achievement of emission reduction targets.
Surfactant deficiency increases SARS-CoV-2 canonical and non-canonical viral entry to lung epithelial cells and exacerbates intrapulmonary inflammatory responses
Authors: Leibel Sandra , McVicar Rachael , Kwong Elizabeth , Clark Alex , Alvarado Asuka , Grimmig Bethany , Nuryyev Ruslan , Young Randee , Lee Jamie , Peng Weiqi , Zhu Yanfang , Griffis Eric , Nowell Cameron , James Brian , Alarcon Suzie , Malhotra Atul , Gearing Linden , Hertzog Paul , Galapate Cheska , Galenkamp Koen , Commisso Cosimo , Smith Davey , Sun Xin , Carlin Aaron , Croker Ben , Snyder Evan 
Published: April 2023

Abstract

The prevalence of “Long COVID”, including among vaccinated patients, is just one of the conundrums that indicate how much remains unknown about the lung’s response to viral infection, particularly to SARS-CoV-2 for which the lung is the point of entry. Therefore, we used an in vitro human lung system to enable a prospective, unbiased, sequential single cell level analysis of pulmonary cell responses following infection by multiple strains of SARS-CoV-2. By starting with human induced pluripotent stem cells (hiPSCs) and emulating lung organogenesis, three-dimensional lung organoids were generated and infected in which several unexpected but pertinent insights emerged. First, SARS-CoV-2 tropism is much broader than previously believed: most lung cell types can be infected, if not through a canonical receptor-mediated route (e.g., via ACE2) then via a non-canonical “backdoor” endocytosis/micropinocytosis route. Such entry can be abrogated by FDA-approved endocytosis blockers, suggesting novel adjunctive therapies. Regardless of route-of-entry, the virus triggers a heretofore unrecognized lung epithelial cell-intrinsic autonomous innate immune response involving interferons and cytokine/chemokine production in the absence of hematopoietic cells or their derivatives. The virus can spread rapidly throughout human lung organoid cell cultures resulting in mitochondrial apoptosis mediated by the pro-survival protein Bcl-xL. This host cytopathic response to the virus may help explain persistent inflammatory signatures in a dysfunctional pulmonary environment of long COVID. The host response to the virus is, in part, dependent on the presence of pulmonary Surfactant Protein-B (SP-B), which plays an unanticipated role in signal transduction, viral resistance, dampens systemic inflammatory cytokine production, and minimizes the induction of apoptosis.
Extreme hepatectomy with modified ALPPS in a rat model: Gradual portal vein restriction associated with hepatic artery restriction
Authors: He Xiaoqin , Zhang Yuefeng , Ma Peng , Mou Zuo , Wang Wei , Yu Kaihuan , Wang Weixing 
Published: April 2023

Abstract
Background & aim: Associating liver partition and portal vein ligation (PVL) for staged hepatectomy (ALPPS) is a creative strategy for enlarging the future liver remnant (FLR) and increasing the tumor resectability rate. However, the indications for ALPPS must have a certain limit when the FLR is too small. We aimed to establish a modified ALPPS model with more widen applicability in rats.
Methods: An extreme ALPPS model was established in rodents with only a 6.5% FLR. The portal vein (PV) was subjected to restriction to different degrees, then the portal vein pressure (PVP) was measured. Then, different modifications of ALPPS, including hepatic artery restriction (HAR), gradual portal vein restriction (GPVR), and GPVR-associated HAR (HAR+GPVR), were applied in the extreme ALPPS models.
Results: PVL or PVR provoked an immediate increase in the PVP. The PVP in the PVR -1.28 mm, PVR -0.81 mm, PVR -0.63 mm, and PVL groups was 11.05±1.57 cmH2O, 16.18±1.92 cmH2O, 20.66±1.99 cmH2O, and 24.10±3.33 cmH2O, respectively, and the corresponding 3-day survival rate was 100%, 90.09%, 36.33% and 0, respectively. Then, in the extreme ALPPS model, the growth ratio of the FLR in the control, HAR, GPVR, and HAR+GPVR groups was 0.43±0.21, 0.50±0.16, 4.80±0.86, and 7.40±2.56, and as a consequence, the corresponding 30-day survival rate was 9.09%, 15.38%, 84.61% and 92.90%, respectively.
Conclusion: ALPPS itself has a limit, and high PVP after PVL contributes to postoperative death in the extreme ALPPS model. Furthermore, a modified method for extreme ALPPS is proposed, i.e., GPVR+HAR in place of PVL, which significantly improves the survival rate of extreme hepatectomy in rat models.