bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel findings about the mode of action of the antifungal protein PeAfpA against Saccharomyces cerevisiae
Authors: Giner-Llorca Moisés , Locascio Antonella , del Real Javier Alonso , Marcos Jose F. , Manzanares Paloma 
Published: September 2023

Abstract
Antifungal proteins (AFPs) from filamentous fungi offer the potential to control fungal infections that threaten human health and food safety. AFPs exhibit broad antifungal spectra against harmful fungi, but limited knowledge of their killing mechanism hinders their potential applicability. PeAfpA from Penicillium expansum shows strong antifungal potency against plant and human fungal pathogens and stands above other AFPs for being active against the yeast Saccharomyces cerevisiae. We took advantage of this and used a model laboratory strain of S. cerevisiae to gain insight into the mode of action of PeAfpA by combining (i) transcriptional profiling, (ii) PeAfpA sensitivity analyses of deletion mutants available in the S. cerevisiae genomic deletion collection and (iii) cell biology studies using confocal microscopy. Results highlighted and confirmed the role of the yeast cell wall (CW) in the interaction with PeAfpA, which can be internalized through both energy-dependent and independent mechanisms. The combined results also suggest an active role of the CW integrity (CWI) pathway and the cAMP-PKA signalling in the PeAfpA killing mechanism. Besides, our studies revealed the involvement of phosphatidylinositol metabolism and the participation of ROX3, which codes for the subunit 19 of the RNA polymerase II mediator complex, in the yeast defence strategy. In conclusion, our study provides clues about both the killing mechanism of PeAfpA and the fungus defence strategies against the protein, suggesting also targets for the development of new antifungals.

Key points
• PeAfpA is a cell-penetrating protein with inhibitory activity against S. cerevisiae.
• The CW integrity (CWI) pathway is a key player in the PeAfpA killing mechanism.
• Phosphatidylinositol metabolism and ROX3 are involved in the yeast defence strategy.
Thermal treatment of the tourmaline Fe-rich princivalleite Na(Mn2Al)Al6(Si6O18)(BO3)3(OH)3O
Authors: Altieri Alessandra , Luppi Riccardo , Skogby Henrik , Hålenius Ulf , Tempesta Gioacchino , Pezzotta Federico , Bosi Ferdinando 
Published: September 2023

AbstractNatural Fe2+-rich princivalleite was thermally treated in the air at 700 °C to study crystal-chemical and color variations due to changes in oxidation states of Fe and Mn and atom ordering. Overall, the experimental data (electron microprobe, structural refinement, Mössbauer, infrared, and optical absorption spectroscopy) show that thermal treatment of princivalleite results in an almost total Fe2+ oxidation to Fe3+ and an oxidation of approximately one-third of Mn2+ to Mn3+ along with a minor degree of disorder of Al–Fe–Mn over the Y and Z sites. This process is accompanied by a significant deprotonation of the sample. The YFe and YMn oxidation from + 2 to + 3 yields in a decrease in a-parameter, whereas the increased content of ZFe3+ results in a minor increase in the c-parameter. Optical absorption spectroscopy shows that the faint blue (azure) color of untreated princivalleite is caused by the presence of Fe2+ and the absence of Ti4+. Thermal treatment in air (700 °C) changed the color to dark brown due to the progressive oxidation of Fe2+ to Fe3+ and Mn2+ to Mn3+, as demonstrated by the evolution of optical absorption bands caused by electron transitions in these 3d-cations. However, the most evident result of the thermal treatment of the Fe-rich princivalleite sample is the simultaneous presence of Fe2+, Fe3+, Mn2+, and Mn3+, with a Fe3+/ΣFe and Mn3+/ΣMn ratio of 0.92 and 0.25, respectively. This observation suggests that the oxidation process during the heating experiments was largely controlled by kinetic factors.
Optimizing congenital cytomegalovirus detection by pool testing in saliva by a rapid molecular test
Authors: Izquierdo Giannina , Farfan Mauricio J , Villavicencio Leonel , Montecinos Luisa , Tarque Felipe , Acevedo William , Reyes Roberto , Guerra Carolina , Araya Leslie , Sepúlveda Belén , Cabrera Camila , Medina Pamela , Mendez Jocelyn , Mardones Elieder , Torres Juan P 
Published: September 2023

AbstractUniversal congenital cytomegalovirus (cCMV) screening in saliva is increasingly recommended. The aim of our study was to correlate the performance of a point-of-care rapid molecular test with CMV real time PCR (CMV RT-PCR) detection, using saliva pool-testing in newborns under a universal screening strategy. Saliva swabs were prospectively collected from newborns < 21 days old and tested by Alethia-LAMP-CMV assay in pools of 5 samples. In positive pools, subjects were tested individually and by saliva and urine CMV RT-PCR. A subset of negative pools were studied with both techniques and viral loads in whole blood were determined in positive patients. From 1,642 newborns included in 328 pools, 8 were confirmed by urine CMV RT-PCR, (cCMV prevalence 0,49%). The PPA and NNA of the pooled saliva Alethia-LAMP-CMV testing were 87,5% and 99,8% with a negative and positive predictive value of 99,9% and 77,7%, respectively. Two false positives were detected (0,12%). A subset of 17 negative pools (85 samples), studied by saliva CMV RT-PCR, showed 100% concordance. Conclusion: CMV pool-testing using a rapid molecular test in saliva proved feasible when compared to PCR gold standards. This strategy could improve cost-effectiveness for cCMV universal neonatal screening, based on the low prevalence of the infection and could be a more affordable approach in less developed regions with reduced detection capacity.

What is Known:• cCMV is the most frequent congenital infection and a leading nongenetic cause of sensorineural hearing loss and brain disease.
• Universal screening could allow early detection of congenitally infected infants, improving clinical outcome.

• Saliva PCR is the preferred and non-invasive test for newborn cCMV screening.

What is New:
• The feasibility of a universal cCMV screening by pool-testing in saliva using a rapid test in pools of 5 samples.

• PPA and NPA were 87,5 and 99,8% compared to CMV PCR in urine.

• This strategy could be relevant specially in LMIC where detection capacity is reduced and could improve cost-effectiveness.

• cCMV prevalence in our center was 0,49%.
The influence of volcano topographic changes on infrasound amplitude: lava fountains at Mt. Etna in 2021
Authors: Iozzia Adriana , Watson Leighton M. , Cantarero Massimo , De Beni Emanuela , Di Grazia Giuseppe , Ganci Gaetana , Johnson Jeffrey B. , Privitera Eugenio , Proietti Cristina , Sciotto Mariangela , Cannata Andrea 
Published: September 2023

AbstractInfrasound signals are used to investigate and monitor active volcanoes during eruptive and degassing activity. Infrasound amplitude information has been used to estimate eruptive parameters such as plume height, magma discharge rate, and lava fountain height. Active volcanoes are characterized by pronounced topography and, during eruptive activity, the topography can change rapidly, affecting the observed infrasound amplitudes. While the interaction of infrasonic signals with topography has been widely investigated over the past decade, there has been limited work on the impact of changing topography on the infrasonic amplitudes. In this work, the infrasonic signals accompanying 57 lava fountain paroxysms at Mt. Etna (Italy) during 2021 were analyzed. In particular, the temporal and spatial variations of the infrasound amplitudes were investigated. During 2021, significant changes in the topography around the most active crater (the South East Crater) took place and were reconstructed in detail using high resolution imagery from unoccupied aerial system surveys. Through analysis of the observed infrasound signals and numerical simulations of the acoustic wavefield, we demonstrate that the observed spatial and temporal variation in the infrasound signal amplitudes can largely be explained by the combined effects of changes in the location of the acoustic source and changes in the near-vent topography, together with source acoustic amplitude variations. This work demonstrates the importance of accurate source locations and high-resolution topographic information, particularly in the near-vent region where the topography is most likely to change rapidly and illustrates that changing topography should be considered when interpreting local infrasound observations over long time scales.
Revised and wider classes of isotropic space-time covariance functions
Authors: Posa D. 
Published: September 2023

AbstractSeveral classes of space-time correlation models have been proposed by various authors in the last years. However, most of these families utilize non negative covariance functions to be adapted to different case studies: indeed, the traditional classes of covariances, such as the Whittle–Matern class and the several families constructed by applying the classical properties are not so flexible to describe covariance functions characterized by negative values. A recent analysis, regarding the difference between two isotropic covariance functions, has underlined that these new families of models are more flexible than the traditional ones because the same models are able to select covariance functions which are always positive in their domain, as well as covariance functions which could be negative in a subset of their field of definition. Moreover, within the same class of models, it is possible to select covariance models which present different behaviours in proximity of the origin. In this paper several families of isotropic space-time covariance functions, among the ones proposed in the literature, have been reviewed in order to enrich the same families including models characterized by negative values in a subset of their domain. Furthermore, the definition of separability has been revised in order to enlarge the classical definition. Apart from the theoretical importance related to the new aspects, these new classes of covariance models are characterized by an extremely simple formalism and can be easily adapted to several case studies.
Hidden in plain sight: how individual ADHD stakeholders have conflicting ideas about ADHD but do not address their own ambivalence
Authors: van Langen Myrte J. M. , van Hulst Branko M. , Durston Sarah 
Published: September 2023

AbstractPsychiatric classifications refer to clusters of behavioral symptoms. We know much about how psychiatric classifications are intended to be used in theory. Yet the scientific study of thepractice of classificationto date is limited. We aimed to explore how individuals navigate and make sense of the complexity surrounding an ADHD classification. We used thematic analysis to analyse stakeholder perspectives from seven focus groups: adults classified with ADHD, adolescents classified with ADHD, parents of children classified with ADHD, clinicians, researchers, teachers, and policy makers. We found seven themes in how stakeholders navigate the classification ADHD. Yet, what stood out was an overarching discursive pattern: individual stakeholders expressed highly ambivalent ideas about ADHD but did not address their own ambivalence. We suggest that promoting a social kinds perspective on ADHD may help us navigate the complexity and ambivalence associated with ADHD more competently.
An integrated perspective of electrochemistry teaching in science and technology
Authors: Brett Christopher M. A. , Oliveira-Brett Ana Maria 
Published: September 2023

AbstractA perspective on the teaching of electrochemistry for science and engineering students is discussed followed by the elements and components of a wide-ranging course that can be adapted to the needs of each science and/or engineering students and, indeed, the scientific community and society at large. For all, there is a pressing need to be able to reflect critically on the knowledge gained, so that in their future careers they are able to take scientific, political and economic decisions involving different aspects of electrochemistry in the best way, particularly regarding energy, materials, health, foods and environment.
Three separate acquisitions of blaNDM-1 in three different bacterial species from a single patient
Authors: Mataseje L. F. , Pitout J. , Croxen M. , Mulvey M. R. , Dingle T. C. 
Published: September 2023

AbstractTo investigate the acquisition and relatedness of New Delhi Metallo-beta-lactamase among multiple separate species from one patient. Five isolates from three species (Pseudomonas aeruginosa; Pa, Acinetobacter baumannii; Ab and Proteus mirabilis; Pm) suspected of harbouring a carbapenemase were investigated by phenotype (antimicrobial susceptibilities) and whole genome sequencing. Epidemiological data was collected on this patient. Three different carbapenemase genes were detected; blaVIM-1 (Pa; ST773), blaOXA-23 (Ab, ST499) and blaNDM-1 identified in all isolates. NDM regions were found chromosomally integrated in all isolates. Data showed no evidence of NDM-1 transfer within this patient suggesting the enzyme was acquired in three separate events.
Understanding Thermally Driven Slope Winds: Recent Advances and Open Questions
Authors: Farina Sofia , Zardi Dino 
Published: September 2023

AbstractThe paper reviews recent advances in our understanding about the dynamics of thermally driven winds over sloping terrain. Major progress from recent experiments, both in the field and in the laboratory, are outlined. Achievements from numerical modelling efforts, including both parameterized turbulence and large eddy simulation approaches, up to direct numerical simulations, are also reviewed. Finally, theoretical insights on the nature of turbulence in such winds are analyzed along with applications which benefit from progress in understanding of these flows. Open questions to be faced for further investigations are finally highlighted.
Prognostic impact of adjuvant endocrine therapy for estrogen receptor-positive and HER2-negative T1a/bN0M0 breast cancer
Authors: Sasada Shinsuke , Kondo Naoto , Hashimoto Hiroya , Takahashi Yuko , Terata Kaori , Kida Kumiko , Sagara Yasuaki , Ueno Takayuki , Anan Keisei , Suto Akihiko , Kanbayashi Chizuko , Takahashi Mina , Nakamura Rikiya , Ishiba Toshiyuki , Tsuneizumi Michiko , Nishimura Seiichiro , Naito Yoichi , Hara Fumikata , Shien Tadahiko , Iwata Hiroji 
Published: September 2023

Abstract
Purpose
Mammography screening has increased the detection of subcentimeter breast cancers. The prognosis for estrogen receptor (ER)-positive and human epidermal growth factor receptor 2 (HER2)-negative T1a/bN0M0 breast cancers is excellent; however, the necessity of adjuvant endocrine therapy (ET) is uncertain.

Methods
We evaluated the effectiveness of adjuvant ET in patients with ER-positive and HER2-negative T1a/bN0M0 breast cancer who underwent surgery from 2008 to 2012. Standard ET was administrated after surgery. The primary endpoint was the cumulative incidence of distant metastasis. All statistical tests were 2-sided.

Results
Adjuvant ET was administered to 3991 (83%) of the 4758 eligible patients (1202 T1a [25.3%] and 3556 T1b [74.7%], diseases). The median follow-up period was 9.2 years. The 9-year cumulative incidence of distant metastasis was 1.5% with ET and 2.6% without ET (adjusted subdistribution hazard ratio [sHR], 0.54; 95% CI, 0.32–0.93). In multivariate analysis, the independent risk factors for distant metastasis were no history of ET, mastectomy, high-grade, and lymphatic invasion. The 9-year overall survival was 97.0% and 94.4% with and without ET, respectively (adjusted HR, 0.57; 95% CI, 0.39–0.83). In addition, adjuvant ET reduced the incidence of ipsilateral and contralateral breast cancer (9-year rates; 1.1% vs. 6.9%; sHR, 0.17, and 1.9% vs. 5.2%; sHR, 0.33).

Conclusions
The prognosis was favorable in patients with ER-positive and HER2-negative T1a/bN0M0 breast cancer. Furthermore, adjuvant ET reduced the incidence of distant metastasis with minimal absolute risk difference. These findings support considering the omission of adjuvant ET, especially for patients with low-grade and no lymphatic invasion disease.
Implementing Suicide Risk Screening in a Virtual Addiction Clinic
Authors: Glasner Suzette , Wei August X. , Ryan Patrick C. , Michero Darcy N. , Monico Laura B. , Pielsticker Peyton E. , Horowitz Lisa M. 
Published: September 2023

AbstractThe purpose of this study was to describe the feasibility of implementing suicide risk screening in a virtual addiction clinic. Suicide risk screening was implemented in a virtual addiction clinic serving individuals with substance use disorders (SUD) using a quality improvement framework. One-hundred percent (252/252) of eligible patients enrolled in the clinic were screened for suicide risk (44% female; M[SD] age = 45.0[11.0] years, range = 21–68 years). Nineteen patients (8%) screened positive for suicide risk. After screening, no patients required emergency suicide interventions (100% non-acute positive). Notably, 74% (14/19) of those who screened positive did so by endorsing at least one past suicide attempt with no recent ideation. Suicide risk screening in virtual addiction clinics yields important clinical information for high-risk SUD populations without overburdening workflow with emergency services. Given the high proportion of non-acute positive screens based on suicide attempt histories with no recent ideation, clinicians may utilize information on suicide attempt history to facilitate further mental healthcare.
Media and Information Literacy in the Prescribed Curriculum: A Systematic Review on its Integration
Authors: Rojas-Estrada Elizabeth-Guadalupe , Aguaded Ignacio , García-Ruiz Rosa 
Published: September 2023

Abstract The curricular integration of Media and Information Literacy (MIL) is crucial for cultivating informed, critical, and engaged citizens in contemporary society. It assists in addressing the challenges of the digital era and capitalizing on the opportunities presented by the ever-changing media landscape. Thus, the present systematic literature review uses the PRISMA guidelines to examine three dimensions in the process of integration of Media and Information Literacy (MIL) in the prescribed curriculum: formulation, implementation, and evaluation and challenges. Starting with the search criteria, 131 studies were found in the Web of Science, Scopus, ERIC, Dialnet and Google Scholar databases, published between January, 2013, and March, 2023, written in English, Spanish, Portuguese, and Turkish. The findings suggest that the will of the political sphere and the activism of the triad composed by passionate teachers, civil society, and academia, are key factors for promoting the introduction of MIL in formal education. Likewise, it is underlined that the evaluation of this education policy requires special attention, in order to guarantee the analysis of its reach, effectiveness, and capacity to adapt against the challenges that emerge in the media ecosystem. Thus, the intention is to provide up-to-date information for the creation of policies, research studies, and curricular content on this subject.
Renewable energy deployment in Europe: Do politics matter?
Authors: Silva Nuno , Fuinhas José Alberto , Koengkan Matheus , Kazemzadeh Emad , Kaymaz Volkan 
Published: September 2023

AbstractThis study analyzes non-hydroelectric renewable capacity across 27 European countries from 2000 to 2020, using advanced econometric techniques like the Dumitrescu and Hurlin panel Granger non-causality method, Panel Autoregressive Distributed Lag and Panel Quantile Autoregressive Distributed Lag estimations. This investigation reveals complex relationships extending beyond immediate variables. These relationships, rooted in pairwise causalities and broader interactions, underpin observed phenomena. Causality tests show that achieving non-hydroelectric renewable capacity is a long-term endeavor, emphasizing persistent policy approaches for effective energy transition. Three key variables emerge as potent policy drivers: maintaining an environment conducive to economic freedom, fostering financial development, and driving non-hydroelectric renewable patents’ research and development. These variables play a pivotal role in capacity expansion. Additionally, the findings of this empirical investigation spotlight the role of political orientations. Leftist governments have lagged in prioritizing energy transition, prompting questions about neglecting environmental concerns and necessitating comprehensive policy reform. In essence, this study offers novel insights into non-hydroelectric renewable energy deployment. By unraveling complex relationships, emphasizing persistent policies, and identifying key variables, this investigation provides a nuanced perspective in line with sustainable energy transition urgency. As global ecological imperatives heighten, our work guides informed policy decisions for a greener future.
The spatial nexus of monuments, memorability and identity formation in Chatsworth and Durban Central, South Africa
Authors: Mukwidigwi Tariro , Naidu Maheshvari , Govender Subashini , Membele Garikai 
Published: September 2023

AbstractThe monumentalization of public space has received significant scholarly attention both globally and locally. In the South African context, this interest has been further heightened by the #Rhodes Must Fall campaign in 2015, which raised questions about the relevance of colonial statues in the post-transformation era. This campaign sparked extensive debates regarding monumentalization within the country’s cultural landscape. Against this backdrop, the present empirical study aims to contribute to these discussions by examining the role of monuments, particularly their spatial characteristics, in shaping processes of memory and identity reconstruction within urban public spaces. The study employed cultural mapping techniques, including participatory GIS (PGIS) and semi-structured interviews, to generate data in the areas of Chatsworth and Durban Central. Additionally, the study draws upon Henri Lefebvre’s (1974) conceptualizations of monuments and Proshansky’s (1983) place-identity theory to explore the constitutive relationship between the spatiality of monuments and embodied memories in the production of spatial identities. The empirical findings of the study affirm monuments as sites for negotiating spatial identities, acknowledging the diversity and multiplicity of memories and identities that emerged. Furthermore, the study highlights the significance of the spatial context of monuments, including dimensions such as aesthetic appeal, geographic prominence, positionality, and discernibility, in influencing the symbolic value of monuments in memory-making and identity formation processes. In conclusion, the paper emphasizes the importance of reconfiguring the spatial context of monuments to ensure their efficacy in processes of memorialization and identity reconstruction.