bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immersive Videography of Ancient DNA Extraction for Community Engagement and Educational Initiatives by the Anson Street African Burial Ground Project
Authors: Fleskes Raquel E. , Gilmore Joanna K. , Oubré La' Sheia , Ofunniyin Ade A. , Cabana Graciela S. , Schurr Theodore G. 
Published: January 2025

ABSTRACTObjectiveCommunity engagement is an increasingly important component of ancient DNA (aDNA) research, especially when it involves archeological individuals connected to contemporary descendants or other invested communities. However, effectively explaining methods to non‐specialist audiences can be challenging due to the intricacies of aDNA laboratory work. To overcome this challenge, the Anson Street African Burial Ground (ASABG) Project employed a GoPro camera to visually document the process of aDNA extraction for use in community engagement and education events.MethodsA GoPro Hero 6 camera enclosed in a decontaminated underwater case was used to film multiple rounds of aDNA extractions from first‐ and third‐person perspectives. The raw footage was edited into long (13‐minute) and short (5‐minute) format videos to summarize the steps of aDNA extraction for different educational aims.ResultsThe videos were used at community engagement events, as well as in classrooms and other educational venues for students of different age groups. General feedback from the community was solicited at the events. We found that the use of videographic methods increased the transparency and accessibility of the aDNA research conducted by the ASABG Project team.DiscussionProviding a visual guide to the often destructive nature of aDNA testing served as an important step in the continuing practice of informed (dynamic) consent with the descendant community. Future initiatives could expand these visualization efforts by illustrating other steps in the aDNA testing process, such as library preparation or sequencing, or incorporating approaches such as live streaming to foster trust and expand public science literacy.
A Needle in a Haystack: Transient Porosity in a Closed Pore Square Lattice Coordination Network
Authors: Koupepidou Kyriaki , Eaby Alan C. , Sensharma Debobroto , Javan Nikkhah Sousa , He Tao , Lusi Matteo , Vandichel Matthias , Barbour Leonard J. , Mukherjee Soumya , Zaworotko Michael J. 
Published: January 2025

AbstractGuest transport through discrete voids (closed pores) in crystalline solids is poorly understood. Herein, we report the gas sorption properties of a nonporous coordination network, {[Co(bib)2Cl2] ⋅ 2MeOH}n (sql‐bib‐Co‐Cl‐α), featuring square lattice (sql) topology and the bent linker 1,3‐bis(1H‐imidazol‐1‐yl)benzene (bib). The as‐synthesized sql‐bib‐Co‐Cl‐α has 11.3 % (313 Å3) of its unit cell volume in closed pores occupied by methanol (MeOH). Upon desolvation and air exposure, sql‐bib‐Co‐Cl‐α underwent a single‐crystal to single‐crystal (SC‐SC) phase transformation to sql‐bib‐Co‐Cl‐β′, wherein MeOH was replaced by water. Activation (vacuum or N2 flow) resulted in dehydration and retention of the closed pores, affording sql‐bib‐Co‐Cl‐β with 7.7 % (194 Å3) guest‐accessible space. sql‐bib‐Co‐Cl‐β was found to preferentially adsorb C2H2 (at 265 K) over CO2 (at 195 K) through gate‐opening mechanisms, at gate‐opening pressures of 59.8 and 27.7 kPa, respectively, while other C2 gases were excluded. PXRD was used to monitor transformations between the three phases of sql‐bib‐Co‐Cl, while in situ DSC, in situ SCXRD under CO2 pressure, and computational studies provided insight into the guest transport mechanism, which we attribute to the angular, flexible nature of the bib ligand. Further, the preferential adsorption of C2H2 over CO2 and other C2 gases suggests that transiently porous sorbents might have utility in separations.
Apprehending siRNA Machinery and Gene Silencing in Brinjal Shoot and Fruit Borer, Leucinodes orbonalis
Authors: Reddy N. Veeramanikanta , Suman T. C. , Gandhi Gracy R. , Pathak Jyoti , Yadu Y. K. , Venkatesan T. , Sushil Satya Nand 
Published: January 2025

ABSTRACTRNA interference (RNAi) technology is widely used in gene functional studies and has been shown to be a promising next generation alternative for insect pest management. To understand the efficiency of RNAi machinery in Leucinodes orbonalis (L. orbonalis) Guenee, a destructive pest of eggplant, core RNAi pathway genes Argonaute‐2, Dicer‐2, Loquacious, and Sid‐1 were mined from the transcriptome and characterized. The transcript abundance of these genes was studied after exposure to exogenous double‐stranded RNA (dsRNA). Domain structure analysis revealed that these genes have conserved domains required for the definite protein function in the siRNA pathway. The protein sequences when subjected to phylogenetic analysis showed a close relation with homologs obtained from Ostrinia sp. The insects fed with dsRNA designed for vacuolar sorting protein SNF7 gene showed significant downregulation at 48 h post treatment and about 79% larval mortality. The expression study of genes showed a significant spike in transcript abundance of Dicer‐2, Argonatute‐2, and downregulation of Loquacious at 24 and 48 h post dsRNA exposure. The results on siRNA machinery genes expression and target gene knockdown implies L. orbonalis has an ample response to exogenous dsRNA.
An Immunosenescent CD8+ T Cell Subset in Patients with Axial Spondyloarthritis and Psoriatic Arthritis Links Spontaneous Motility to Telomere Shortening and Dysfunction
Authors: Paldino Giorgia , Tedeschi Valentina , Proganò Valentina , Salvati Erica , Licursi Valerio , Vertecchi Eleonora , Bivolaru Alexandru L. , Molteni Emanuele , Scrivo Rossana , Congia Mattia , Cauli Alberto , Caccavale Rosalba , Paroli Marino , Kunkl Martina , Tuosto Loretta , Sorrentino Rosa , Fiorillo Maria Teresa 
Published: January 2025

ObjectiveA pathogenetic role of CD8+ T lymphocytes in radiographic axial spondyloarthritis (r‐axSpA) and other spondyloarthritis (SpA) is sustained by genome‐wide association studies and by the expansion of public T cell clonotypes in the target tissues. This study investigates the migration of CD8+ T cells along with their phenotype and functions in patients with r‐axSpA and psoriatic arthritis (PsA).MethodsPeripheral blood CD8+ and CD4+ T cells were isolated from patients with r‐axSpA (n = 128), PsA (n = 60), and rheumatoid arthritis (RA) (n = 74) and healthy donors (HDs) (n = 79). Transwell migration assay was performed in the presence of different chemokines. CD8+ T cell immunoprofiling and effector functions were assessed by multiparametric flow cytometry. Transcriptome signature was evaluated by RNA sequencing analysis, whereas telomere length and dysfunction were measured by reverse transcriptase–polymerase chain reaction and immunofluorescence‐fluorescence in situ hybridization, respectively.ResultsA significantly higher number of CD8+ T cells migrating in the absence of chemokine stimuli was found in patients with SpA compared with HDs and patients with RA. This subset, producing cytotoxic (granzyme B, perforin, granulysin) and proinflammatory molecules (tumor necrosis factor), was significantly enriched in terminally differentiated (CCR7−CD45RA+) and senescent (CD28−CD57+) cells having a gene expression profile characterized by cytolytic signature and natural killer markers. Remarkably, these spontaneously migrating CD8+ T cells showed DNA damage response activation, telomere shortening, and dysfunction.ConclusionThese data describe a terminally differentiated CD8+ T cell subset with a senescent and cytotoxic/proinflammatory profile and an intrinsic invasive potential enriched in patients with SpA that represents a possible player in disease pathogenesis.
Nanoarchitectonics for Regulating Molecular Conductance by Multi‐Channel Structure
Authors: Li Hui‐Xin , Liu Jiao‐Yang , Geng Bi‐Jun , Qiu Sheng‐Jie , Chen Zhong‐Ning , Yang Yang , Zhang Qian‐Chong 
Published: January 2025

AbstractMolecular electronics represents the cutting‐edge and interdisciplinary effort on the future miniaturization of electronic circuits. Benefiting from synthetic chemistry and theoretical insights, molecular circuit studies have promoted devices with increasingly complicated structures. Especially, the evolution of conductive backbones from simple chain‐shaped single‐channel configurations to complex multi‐channel architectures marks a pivotal progression. A comprehensive understanding of charge transport and conductance properties in multi‐channel molecular devices is crucial for further developing molecular circuits. In this review, we provide an overview of conductance properties, categorizing the effect on conductance into enhancement or suppression. The underlying mechanisms of conductance modulation are discussed. While conductance enhancement is attributed to factors of the quantum‐interference‐based superposition law, the charge‐induced self‐gating effect, and the facilitation of additional conductive channels through space‐mediated transport; while the conductance suppression originates from the destructive quantum interference in saturated conductive channels and the “electron selective transport” feature within multi‐channel structures where channels converge at a phenyl group.
Influence of Cell Physiological Status on the Intensified Fed‐Batch Cultures at Ultra‐High Seeding Density
Authors: Ran Qingyuan , Su Ying , Zhang Weijian , Zhang Xinran , Zhao Liang , Chen Min , Wan Yuxiang , Tan Wen‐song , Ye Qian 
Published: January 2025

ABSTRACTRecent years, intensified fed‐batch culture with ultra‐high seeding density (uHSD‐IFB) is coming to the forefront of manufacturers’ choice for its enhanced productivity. However, the effects of seed cell physiological state and aeration strategies on these processes remain underexplored due to the ultra‐high seeding density. Currently, the pre‐production seeding inoculum (N‐1) crucial for the uHSD‐IFB cultures relies heavily upon case‐by‐case empirical experiences. To develop a rational seeding approach as a guideline, we here explored the impact of perfusion rates and cell growth states on the subsequent uHSD‐IFB processes. It was found that seed cells in the exponential growth phase with high perfusion rates in the N‐1 perfusion stage allowed for higher viable cell density and titer in the production stage. In particular, lower levels of reactive oxygen species, higher proportions of G1 and S phase, and higher specific cell oxygen uptake rates (OURs) were exhibited in these cells, resulting in higher cell specific growth rates and integral of viable cell concentration (IVCC) throughout the production cultures. Further investigation into the effect of aeration strategies was carried out in the benchtop bioreactors. A final yield of 4.5 g/L, an increase of nearly 110%, was achieved by a sophisticated dual sparger system compared to the other two processes with either one l‐shaped or micro‐sparger. These results provide a direction for the design and establishment of high‐titer processes in intensified fed‐batch cultures at ultra‐high seeding density.Synopsis: In this work, we first explored the impact of perfusion rates and cell growth states on the subsequent uHSD‐IFB processes. Further investigation into the effect of aeration strategies of intensified fed‐batch process was carried out in the benchtop bioreactors. These results provide a direction for the design and establishment of high‐titer processes in intensified fed‐batch cultures at ultra‐high seeding density.
Selective Turn‐On Luminescent Recognition of Perchlorate Ion Using Pyridyl‐Benzimidazole–Based Probe
Authors: Naithani Sudhanshu , Goswami Nidhi , Yadav Vikas , Mangalam Jimmy , Goswami Tapas , Kumar Sushil 
Published: January 2025

ABSTRACTAnions play a crucial role in various environmental, chemical, and biological processes. Among various anions, the production of perchlorate (ClO4−) ion is expected to rise in upcoming years, and thus, an efficient method for the detection of perchlorate ion is highly desirable. In this effort, a pyridyl‐benzimidazole–based luminescent probe (RSB1) containing two N‐H donor sites has been synthesized for selective detection of perchlorate ion. Different spectral techniques such as FT‐IR, NMR, ESI‐mass, UV–Vis, and fluorescence analyses have been used to characterize this probe. High selectivity of RSB1 for ClO4− was realized even in presence of strongly interfering species in aqueous‐acetonitrile (CH3CN‐H2O; 4:1, v/v) solution. Notably, RSB1 served as a “turn‐on” perchlorate‐responsive probe and exhibited an emission enhancement at 363 nm when excited at 300 nm. The detection limit (LoD) and the binding constant (Kb) values were depicted to be 0.121 μM and 2.6 × 105 M−1, respectively, while the binding mechanism for RSB1‐ClO4− was validated via Job's plot, NMR, and DFT analyses. Furthermore, this probe was successfully employed to trace perchlorate in real samples such as tap water, distilled water, and soil samples with good to excellent recovery values.
A Flexible Hybrid Site‐Specific Integration‐Based Expression System in CHO Cells for Higher‐Throughput Evaluation of Monoclonal Antibody Expression Cassettes
Authors: Szkodny Alana C. , Lee Kelvin H. 
Published: January 2025

ABSTRACTThe implementation of site‐specific integration (SSI) systems in Chinese hamster ovary (CHO) cells for the production of monoclonal antibodies (mAbs) can alleviate concerns associated with production instability and reduce cell line development timelines. SSI cell line performance is driven by the interaction between genomic integration location, clonal background, and the transgene expression cassette, requiring optimization of all three parameters to maximize productivity. Systematic comparison of these parameters has been hindered by SSI platforms involving low‐throughput enrichment strategies, such as cell sorting. This study presents a recombinase‐mediated cassette exchange (RMCE)‐capable SSI system that uses only chemical selection to enrich for transgene‐expressing RMCE pools in less than one month. The system was used to compare eight mAb expression cassettes containing two novel genetic regulatory elements, the Azin1 CpG island and the Piggybac transposase 5’ terminal repeat, in various orientations to improve the expression of two therapeutic mAbs from two genomic loci. Similar patterns of productivity and mRNA expression were observed across sites and mAbs, and the best performing cassette universally increased mAb productivity by 7‐ to 11‐fold. This flexible system allows for higher‐throughput comparison of expression cassettes from a consistent clonal and transcriptional background to optimize RMCE‐derived cell lines for industrial production of mAbs.
Management of Radix Entomolaris of Mandibular First Molar With Complex Root Canal Curvatures: A Case Report and Mini Review
Authors: Chamani Ali , Gharechahi Maryam , Shakiba Reza , Alipour Farshad 
Published: January 2025

ABSTRACTRadix entomolaris (RE) characterized by an extra root in mandibular molars is a rare phenotype. When this condition coincides with complex root canal curvatures or S‐shaped canals, root canal therapy (RCT) challenges arise. The present study presents three cases of RE along with complex root curvatures. The challenges of RCT and case management in this study are addressed within the framework of paying attention to the access cavity according to the extra root, instrumentation with the crown‐down technique, sufficient irrigation, using nickel–titanium rotary systems, dense obturation with the warm vertical technique, and patient follow‐up. Also, in the last case, the management of two broken files by loop and bypass techniques is described. Adequate knowledge of the root canal system's morphology and the use of appropriate techniques and materials for root canal therapy are the background for successful RCT.
Mitochondrial Localization of Antioxidant Nanodrug Suppresses Ocular Inflammation by Alleviating Oxidative Stress on Cells
Authors: Ren Zhibin , Liu Xiaoying , Lv Guanghao , Wang Xiaiting , Wang Jingli , Wu Wei , Li Xingyi , Wang Jiaqing 
Published: January 2025

AbstractExternal environments (e. g., pollutants, irritants, ultraviolet radiation, etc) probably activate oxidative stress on the ocular surface, further leading to inflammatory responses and cellular apoptosis. For treating ophthalmic diseases, one of the strategies is to regulate oxidative stress through antioxidants. Here we conjugate a polyphenolic antioxidant drug (i. e., caffeic acid) with a small peptide of protein tag to generate a peptide‐drug conjugate as a nanodrug. With a self‐assembled ability to form nanoparticles, the nanodrug mainly enters human corneal epithelial cells (HCEC) by caveolin‐mediated endocytosis, succeeds lysosomal escape, and achieves mitochondrial localization of caffeic acid. Revealed by free radical scavenging experiments, the nanodrug shows considerable antioxidant capacities. In mouse leukemia cells of monocyte macrophage (RAW 264.7) induced by lipopolysaccharide (LPS), the nanodrug inhibits various pro‐inflammatory cytokines (e. g., NO, IL‐6, and TNF‐α) by up‐regulating the expression of anti‐apoptotic protein (e. g., Bcl‐2). As an investigation of alleviating oxidative stress by the mitochondrial localization of antioxidant, this work may establish an experimental foundation for the regulation of cellular redox balance, as well as provide different insights for the clinical development of antioxidant drug delivery systems in the treatment of ocular disease.
Advances in the Release of Amide‐Containing Molecules
Authors: Wharton Thomas , Spring David R. 
Published: January 2025

AbstractThe ability to release a molecule from a larger construct in a controlled manner is of great importance to produce effective prodrugs, antibody‐drug conjugates, and chemical probes. Amides are ubiquitous functional groups and yet methods to utilise them as molecular release handles are seldom reported. This concept article highlights the advances made in amide release strategies and how these approaches have been utilised.
Effects of Cholesterol on the Breast Cancer Resistance Protein: Studies through the Synthesis and Biological Evaluation of Chemical Tools
Authors: Fatima Zattoni Ingrid , Estelita Rugisnk Bruna , da Silva Zanzarini Isadora , Guilherme Gonçalves Alan , Rotuno Moure Vivian , Valdameri Glaucio , Boumendjel Ahcène 
Published: January 2025

AbstractThe breast cancer resistance protein (BCRP/ABCG2) plays a major role in the multidrug resistance of cancers toward chemotherapeutic treatments. It was demonstrated that cholesterol regulates the ABCG2 activity, suggesting that lower levels of membrane cholesterol decrease the ABCG2 activity in mammalian cells. However, the precise mechanism remains unclear. To better understand the role of cholesterol in the ABCG2 activity, we studied the ABCG2‐mediated efflux of different substrates in the presence of different concentrations of cholesterol. Moreover, we synthetized derivatives of cholesterol linked either to known ABCG2 inhibitors or fluorescents probes. A chalcone‐cholesterol was synthetized to investigate the influence of cholesterol on ABCG2 inhibition, and a BODIPY‐cholesterol was developed to track cholesterol trafficking on mammalian cells and investigate the behavior of cholesterol as an ABCG2 substrate. The obtained results with three different substrates of ABCG2 showed that cholesterol did not affect the intracellular amount of substrates nor the transport activity.
Value of Systematic and MRI‐Ultrasound Fusion Prostate Biopsy in Different Prostate Specific Antigen (PSA) Levels
Authors: Ohadian Moghadam Solmaz , Haddadi Mohammad , Amini Erfan , Momeni Seyed Ali , Bitaraf Masoud , Nowroozi Mohammad Reza 
Published: January 2025

ABSTRACTBackgroundCurrent approach to clinically suspicious biopsy‐naïve men consists performing prostate MRI, followed by combined systematic (TRUS‐Bx) and MRI‐Ultrasound fusion biopsy (MRI‐TBx) in those with PIRADS score ≥ 3. Researchers have attempted to determine who benefits from each biopsy method, but the results do not support the safe use of one method alone. This study aims to determine the optimal approach in biopsy‐naïve men, according to their PSA levels.Methods and ResultsA retrospective chart review of clinically suspicious biopsy‐naïve men who underwent both TRUS‐Bx and MRI‐TBx was done. Prostate specific antigen (PSA) levels were compared between patients only positive for MRI‐TBx and those with positive TRUS‐Bx. Further, cancer cases were divided to < 10 and ≥ 10 PSA groups and the pathology results, obtained by each method, were compared. Out of 195 men, 36 were diagnosed with prostate cancer (PCa). PCa was diagnosed by both MRI‐TBx and TRUS‐Bx in 26 men, half of whom had PSA > 10 ng/mL. At PSA ≤ 10 ng/mL, PCa would have been missed in 4 men (11.1%) had MRI‐TBx not been done, and in 6 men (16.6%) had TRUS‐Bx not been done.ConclusionDespite attempts to perform only one biopsy method in men with clinical suspicion of prostate cancer, we propose that at least in men with PSA ≤ 10 ng/mL, both systematic and MRI‐targeted biopsies be performed.
Uncertainty and Green Innovation Nexus: The Moderating Influence of Ownership Structure and Product Market Competition
Authors: Khan Muhammad Arif , Meng Bin , Ullah Irfan 
Published: January 2025

ABSTRACTRecently, there has been a growing interest among researchers, academics, and corporations regarding whether and how uncertainties influence corporate decision‐making. This study seeks to explore how various forms of uncertainty, including firm‐specific uncertainty (Fs_u), market‐based uncertainty (M_u), and economic policy uncertainty (Ep_u), influence corporate green innovation. This study also examines whether state ownership and product market competition moderate the relationship between uncertainty and green innovation. Using a sample of Chinese‐listed manufacturing firms from 2003 to 2020, the findings show that uncertainties (Fs_u, M_u, and Ep_u) negatively affect corporate green innovation. This negative effect is weakened by state ownership, whereas is strengthened by product market competition. The results remain consistent and valid following thorough robustness checks and additional analyses. This research contributes to real options theory by delineating how internal and external uncertainties impede green innovation.