bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proximity to anthropogenic food sources determine roosting and nesting prevalence of feral pigeons (Columba livia) in a tropical city
Authors: Lim Kai Ning , Soh Malcolm C. K. , Leong Duncan Y. W. , Loo Adrian H. B. , Lee Benjamin P. Y.‐H. , Er Kenneth B. H. 
Published: May 2023

AbstractThe feral pigeon (Columba livia) is a globally commensal bird that can cause dis‐amenities such as soiling and is a potential vector of various diseases. Aside to limiting food as a management strategy, reducing the availability of roosting and nesting sites can help regulate feral pigeon populations. Despite their prevalence, current knowledge of their roosting and nesting preferences is lacking.Feral pigeons commonly use railway viaduct expansion gaps in Singapore for roosting and nesting. These gaps provided an ideal experimental platform to examine feral pigeon roost and nest site selection while controlling for differing cavity sizes which can significantly affect their reproductive success and site selection decisions. We also conducted an in‐situ experiment to test the efficacy of nest removal as a management option.Our nationwide surveys of 80.3 km of railway viaducts and 6048 gaps revealed that feral pigeon day roosting and nesting preferences are influenced by structural height and more importantly, their proximity to human food sources. There was a significantly higher probability of feral pigeon roosting in a gap if it had more pigeon feeding incidences in its vicinity and was higher. The probability of feral pigeon nesting in gap was higher if it was closer to a railway station, lower and further from water bodies.In our field experiment, we did not find any significant differences in the proportions of the abundances of feral pigeon to other urban commensal bird species at the gaps before and after nests were removed.Overall, our results suggest that a concerted effort to reduce anthropogenic food availability to feral pigeons is central in limiting their reproductive success and controlling their population.
Regulating Cathode‐Electrolyte Interphase by Confining Functional Aluminum Compound within Ni‐Rich Cathodes
Authors: Han Yongkang , Zhang Yingchuan , Lei Yike , Xiao Dongdong , Ni Jie , Lin Weiguang , Ming Pingwen , Zhang Cunman , Xiao Qiangfeng 
Published: May 2023

AbstractThe oxidative capability of Ni4+ and high operation voltage of nickel‐rich LiNi1−x−yCoxMnyO2 (Ni‐rich NCM) cause its continuous and deleterious side reactions with electrolyte and irreversible phase transition, which hinder its industrial application. To mitigate these issues, Al (CF3SO3)3 is proposed as a solid electrolyte additive that can be readily oxidized to regulate the cathode‐electrolyte interphase (CEI) due to the highest occupied molecular orbital‐level of CF3SO3−, meanwhile being confined within the single‐crystalline NCM811. CF3SO3− prior to the electrolyte is oxidized upon increasing voltage to produce sulfur components and involve CEI formation. Concurrently, the released Al3+ ions are combined with reactive oxygen from NCM811 particles and HF from the electrolyte to form Al2O3 and AlF3, respectively. A robust sandwich CEI film containing sulfur and aluminum species is formed, which cannot only prevent decomposition of the electrolyte, but also alleviate the formation of inactive rock‐salt phase on NCM811 surface. Consequently, such CEI leads to high‐performance batteries with a high‐capacity retention of 91.5% after 200 cycles under 0.5 C compared to 72.4% of pristine NCM811. This facile and environmentally benign method provides a new avenue to develop high‐capacity and durable cathodes for lithium‐ion batteries.
Engineered Bicomponent Adhesives with Instantaneous and Superior Adhesion Performance for Wound Sealing and Healing Applications
Authors: Zhao Kelu , Li Bo , Sun Yao , Jia Bo , Chen Jing , Cheng Wenhao , Zhao Lai , Li Jingjing , Wang Fan , Su Juanjuan , Sun Jing , Han Bing , Liu Yawei , Zhang Hongjie , Liu Kai 
Published: May 2023

AbstractSurgical adhesives are playing an important role in wound repair and emergency hemostasis in clinical treatment. However, the development of strong bioglue with rapid in situ adhesion, durable adhesiveness, and flexibility in dynamic and moist physiological environments is still challenging. Herein, a new type of biosynthetic protein bioadhesives with superior adhesion performance is reported by developing a protein aldimine condensation strategy. Lysine‐rich recombinant proteins are designed and massively biosynthesized to instantaneously react with aldehyde cross‐linkers to realize in situ strong adhesion. The obtained bioadhesives show an ultra‐high adhesion strength of ≈101.6 kPa on porcine skin, outperforming extant clinical bioglues. In addition, they possess super biocompatibility, flexibility, biodegradability, and compliance with the tissues. Owing to the strong and instantaneous adhesion properties, the bioadhesives are qualified for dynamic wound closure, facilitating wound repair, and noncompressible hemorrhage. Importantly, they can be industrially encapsulated into custom‐made cartridge delivery tubes at low cost for clinical use. Therefore, biosynthetic bioadhesives have great potential for biological applications and are capable of scaling up to the industrial level for clinical transformation, which will be a successful paradigm for reforming existing clinical products.
Programmed Death Ligand‐1‐Overexpressing Donor Exosomes Mediate Donor‐Specific Immunosuppression by Delivering Co‐Inhibitory Signals to Donor‐Specific T Cells
Authors: Luo Xuewei , Du Guicheng , Long Yufei , Zheng Mengchao , Chen Bingye , Li Weiting , Yan Guoliang , Qi Zhongquan , Lan Tianshu 
Published: May 2023

AbstractProgrammed death ligand‐1 (PD‐L1) and donor antigens are critical for donor immature dendritic cells (DCs) targeting donor‐specific T cells to induce transplant tolerance. This study aims to clarify whether DC‐derived exosomes (DEX) with donor antigens (H2b) and high levels of PD‐L1 expression (DEXPDL1+) can help to suppress graft rejection. In this study, it is demonstrated that DEXPDL1+ presents donor antigens, as well as PD‐L1 co‐inhibitory signals, directly or semi‐directly via DCs to H2b‐reactive T cells. This dual signal presentation can prolong the survival of heart grafts from B6 (H2b) mice but not from C3H (H2k) mice by inhibiting T cell activation, inducing activated T cell apoptosis, and modulating the balance of T cell differentiation from inflammatory to regulatory. Additionally, even though DEXPDL1+ treatment cannot induce tolerance after short‐term treatment, this study provides a new vehicle for presenting co‐inhibitory signals to donor‐specific T cells. This novel strategy may facilitate the realization of donor‐specific tolerance via the further optimization of drug‐loading combinations and therapeutic regimens to elevate their killing ability.
Whole Model Path Planning‐Guided Multi‐Axis and Multi‐Material Printing of High‐Performance Intestinal Implantable Stent
Authors: Wang Jiahang , Huang Jinjian , Li Ze , Chen Kang , Ren Huajian , Wu Xiuwen , Ren Jianan , Li Zongan 
Published: May 2023

AbstractThe problems of step effects, supporting material waste, and conflict between flexibility and toughness for 3D printed intestinal fistula stents are not yet resolved. Herein, the fabrication of a support‐free segmental stent with two types of thermoplastic polyurethane (TPU) using a homemade multi‐axis and multi‐material conformal printer guided with advanced whole model path planning is demonstrated. One type of TPU segment is soft to increase elasticity, and the other is used to achieve toughness. Owing to advancements in stent design and printing, the obtained stents present three unprecedented properties compared to previous three‐axis printed stents: i) Overcoming step effects; ii) Presenting comparable axial flexibility to a stent made of a single soft TPU 87A material, thus increasing the feasibility of implantation; and iii) Showing equivalent radial toughness to a stent made of a single hard TPU 95A material. Hence, the stent can resist the intestinal contractive force and maintain intestinal continuity and patency. Through implanting such stents to the rabbit intestinal fistula models, therapeutic mechanisms of reducing fistula output and improving nutritional states and intestinal flora abundance are revealed. Overall, this study develops a creative and versatile method to improve the poor quality and mechanical properties of medical stents.
High Performance Rotary‐Structured Triboelectric‐Electromagnetic Hybrid Nanogenerator for Ocean Wind Energy Harvesting
Authors: Cao Xiaole , Zhou Hanlin , Zhou Yuxuan , Hu Yiran , Wang Yuanyu , Wang Zhong Lin , Sun Qijun 
Published: May 2023

AbstractModern ship is an important transportation and even military equipment on the ocean, which has ubiquitous ocean wind energy with high‐speed and stable wind direction. However, much higher‐speed wind energy during the movement of the ship is often abandoned. Triboelectric nanogenerator (TENG) that can generate electrical energy by scavenging ambient mechanical energy is one of the emerging energy harvesting technologies. By coupling the TENG and electromagnetic generators (EMG), a high‐performance rotary‐structured triboelectric‐electromagnetic hybrid nanogenerator (RS‐HG) is designed in this study. By introducing a matched inductor in the paired energy management circuit (EMC), the charging efficiency of RS‐TENG can be effectively enhanced by 15 times compared to that without EMC. At a simulated wind speed of 15 m s−1, the peak power of lateral TENG (L‐TENG), top TENG (T‐TENG) and EMG are 7.54, 7.85, and 22.5 mW, respectively. The demonstrated RS‐HG comprising two RS‐TENGs and one EMG can readily charge a 1 mF capacitor to 8 V within 15 s under the wind speed of 15 m s−1 and continuously power the electronic devices. Moreover, the RS‐HG can also successfully charge a smartphone through the step‐down and voltage stabilization module to further expand its application scenarios. The applications of powering portable electronics reveal the huge prospects of hybrid nanogenerators in energy harvesting and self‐powered environmental monitoring during navigation.
Amisulpride as a potential disease‐modifying drug in the treatment of tauopathies
Authors: Jahreis Kathrin , Brüge Alina , Borsdorf Saskia , Müller Franziska E. , Sun Weilun , Jia Shaobo , Kang Dong Min , Boesen Nicolette , Shin Seulgi , Lim Sungsu , Koroleva Anastasia , Satała Grzegorz , Bojarski Andrzej J. , Rakuša Elena , Fink Anne , Doblhammer‐Reiter Gabriele , Kim Yun Kyung , Dityatev Alexander , Ponimaskin Evgeni , Labus Josephine 
Published: May 2023

AbstractINTRODUCTIONHyperphosphorylation and aggregation of the microtubule‐associated protein tau cause the development of tauopathies, such as Alzheimer's disease and frontotemporal dementia (FTD). We recently uncovered a causal link between constitutive serotonin receptor 7 (5‐HT7R) activity and pathological tau aggregation. Here, we evaluated 5‐HT7R inverse agonists as novel drugs in the treatment of tauopathies.METHODSBased on structural homology, we screened multiple approved drugs for their inverse agonism toward 5‐HT7R. Therapeutic potential was validated using biochemical, pharmacological, microscopic, and behavioral approaches in different cellular models including tau aggregation cell line HEK293 tau bimolecular fluorescence complementation, primary mouse neurons, and human induced pluripotent stem cell–derived neurons carrying an FTD‐associated tau mutation as well as in two mouse models of tauopathy.RESULTSAntipsychotic drug amisulpride is a potent 5‐HT7R inverse agonist. Amisulpride ameliorated tau hyperphosphorylation and aggregation in vitro. It further reduced tau pathology and abrogated memory impairment in mice.DISCUSSIONAmisulpride may be a disease‐modifying drug for tauopathies.
Photoredox‐Catalyzed Labeling of Hydroxyindoles with Chemoselectivity (PhotoCLIC) for Site‐Specific Protein Bioconjugation
Authors: Singha Roy Soumya Jyoti , Loynd Conor , Jewel Delilah , Canarelli Sarah E. , Ficaretta Elise D. , Pham Quan A. , Weerapana Eranthie , Chatterjee Abhishek 
Published: May 2023

AbstractWe have developed a novel visible‐light‐catalyzed bioconjugation reaction, PhotoCLIC, that enables chemoselective attachment of diverse aromatic amine reagents onto a site‐specifically installed 5‐hydroxytryptophan residue (5HTP) on full‐length proteins of varied complexity. The reaction uses catalytic amounts of methylene blue and blue/red light‐emitting diodes (455/650 nm) for rapid site‐specific protein bioconjugation. Characterization of the PhotoCLIC product reveals a unique structure formed likely through a singlet oxygen‐dependent modification of 5HTP. PhotoCLIC has a wide substrate scope and its compatibility with strain‐promoted azide‐alkyne click reaction, enables site‐specific dual‐labeling of a target protein.
Sterically‐Hindered Molecular p‐Dopants Promote Integer Charge Transfer in Organic Semiconductors
Authors: Charoughchi Somaiyeh , Liu Jiang Tian , Berteau‐Rainville Melissa , Hase Hannes , Askari Mohammad S. , Bhagat Shubham , Forgione Pat , Salzmann Ingo 
Published: May 2023

AbstractMolecular p‐dopants designed to undergo electron transfer with organic semiconductors are typically planar molecules with high electron affinity. However, their planarity can promote the formation of ground‐state charge transfer complexes with the semiconductor host and results in fractional instead of integer charge transfer, which is highly detrimental to doping efficiency. Here, we show this process can be readily overcome by targeted dopant design exploiting steric hindrance. To this end, we synthesize and characterize the remarkably stable p‐dopant 2,2′,2′′‐(cyclopropane‐1,2,3‐triylidene)tris(2‐(perfluorophenyl)acetonitrile) comprising pendant functional groups that sterically shield its central core while retaining high electron affinity. Finally, we demonstrate it outperforms a planar dopant of identical electron affinity and increases the thin film conductivity by up to an order of magnitude. We believe exploiting steric hindrance represents a promising design strategy towards molecular dopants of enhanced doping efficiency.
Amine‐Free, Directing‐Group‐Free and Redox‐Neutral α‐Alkylation of Saturated Cyclic Ketones
Authors: Qiao Jia , Ci Rui‐Nan , Gan Qi‐Chao , Huang Cheng , Liu Zan , Hu Hui‐Lan , Ye Chen , Chen Bin , Tung Chen‐Ho , Wu Li‐Zhu 
Published: May 2023

AbstractThe activation of the α‐C−H bond of ketones typically requires an amine and a directing group to guide the reaction selectivity in amine‐catalysis carbonyl chemistry. For an α‐C−H bond activation of ketone, directing groups are also required to control the reaction selectivity. Reported herein is the first α‐alkylation of cyclic ketones in the absence of an amine catalyst and directing group. 1H NMR, XPS, EPR studies and DFT calculations indicate that an α‐carbon radical intermediate is formed through direct and selective activation of the inert α‐C−H bond of ketones chelating on the surface of colloidal quantum dots (QDs). Such an interaction is essential for weakening the C−H bond, as exemplified, using CdSe QDs as the sole photocatalyst to execute α‐C−H alkylation of cyclic ketones under visible‐light irradiation. Without an amine catalyst and directing group, the high step‐ and atom‐economy transformation under redox‐neutral condition opens a new way for α‐C−H functionalization of ketones in carbonyl chemistry.
Orderly Self‐Assembly of Organic Fluorophores for Sensing and Imaging
Authors: Li Zhe , Liang Ping‐Zhao , Ren Tian‐Bing , Yuan Lin , Zhang Xiao‐Bing 
Published: May 2023

AbstractFluorescence imaging utilizing traditional organic fluorophores is extensively applied in both cellular and in vivo studies. However, it faces significant obstacles, such as low signal‐to‐background ratio (SBR) and spurious positive/negative signals, primarily due to the facile diffusion of these fluorophores. To cope with this challenge, orderly self‐assembled functionalized organic fluorophores have gained significant attention in the past decades. These fluorophores can create nanoaggregates via a well‐ordered self‐assembly process, thus prolonging their residency time within cells and in vivo settings. The development of self‐assembled‐based fluorophores is an emerging field, and as such, in this review, we present a summary of the progress and challenges of self‐assembly fluorophores, focusing on their development history, self‐assembly mechanisms, and biomedical applications. We hope that the insights provided herein will assist scientists in further developing functionalized organic fluorophores for in situ imaging, sensing, and therapy.
Regio‐ and Chemoselective Formal (4+1) Carbocyclization of Chalcones with Internal Alkynes via Rhodium(III) Catalysis
Authors: Song Shuaishuai , Lai Yunfei , Tuo Zekun , Zhong Jianming , Zhou Wang 
Published: May 2023

AbstractA rhodium(III)‐catalyzed oxidative cyclization of chalcones with internal alkynes is reported, generating biologically important 3,3‐disubstituted 1‐indanones along with reusable aromatic aldehydes. This transformation features unique (4+1) reaction mode, excellent regioselectivity in alkyne insertion, broad substrate scope, allows for the construction of quaternary carbon centers, and is scalable. Steric hindrance from substrate and ligand probably controls the chemoselectivity of this carbocyclization. Importantly, this discovery enables a practical two‐step protocol switching the overall reaction of acetophenones with internal alkynes from a (3+2) to a (4+1) annulation.
CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution
Authors: Chen Duo , Huang Wanting , Zhang Yun , Chen Bo , Tan Jie , Yuan Quan , Yang Yanbing 
Published: May 2023

AbstractMass pathogen screening is critical to preventing the outbreaks and spread of infectious diseases. The large‐scale epidemic of COVID‐19 and the rapid mutation of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) virus have put forward new requirements for virus detection and identification techniques. Here, we report a CRISPR‐based Amplification‐free Viral RNA Electrical Detection platform (CAVRED) for the rapid detection and identification of SARS‐CoV‐2 variants. A series of CRISPR RNA assays were designed to amplify the CRISPR‐Cas system‘s ability to discriminate between mutant and wild RNA genomes with a single‐nucleotide difference. The identified viral RNA information was converted into readable electrical signals through field‐effect transistor biosensors for the achievement of highly sensitive detection of single‐base mutations. CAVRED can detect the SARS‐CoV‐2 virus genome as low as 1 cp μL−1 within 20 mins without amplification, and this value is comparable to the detection limit of real‐time quantitative polymerase chain reaction. Based on the excellent RNA mutation detection ability, an 8‐in‐1 CAVRED array was constructed and realized the rapid identification of 40 simulated throat swab samples of SARS‐CoV‐2 variants with a 95.0 % accuracy. The advantages of accuracy, sensitivity, and fast speed of CAVRED promise its application in rapid and large‐scale epidemic screening.
Polyketide Synthase‐Mediated O‐Methyloxime Formation in the Biosynthesis of the Oximidine Anticancer Agents
Authors: Vriens Eveline , De Ruysscher Dries , Weir Angus N. M. , Dekimpe Sofie , Steurs Gert , Shemy Ahmed , Persoons Leentje , Santos Ana Rita , Williams Christopher , Daelemans Dirk , Crump Matthew P. , Voet Arnout , De Borggraeve Wim , Lescrinier Eveline , Masschelein Joleen 
Published: May 2023

AbstractBacterial trans‐acyltransferase polyketide synthases (trans‐AT PKSs) are modular megaenzymes that employ unusual catalytic domains to assemble diverse bioactive natural products. One such PKS is responsible for the biosynthesis of the oximidine anticancer agents, oxime‐substituted benzolactone enamides that inhibit vacuolar H+‐ATPases. Here, we describe the identification of the oximidine gene cluster in Pseudomonas baetica and the characterization of four novel oximidine variants, including a structurally simpler intermediate that retains potent anticancer activity. Using a combination of in vivo, in vitro and computational approaches, we experimentally elucidate the oximidine biosynthetic pathway and reveal an unprecedented mechanism for O‐methyloxime formation. We show that this process involves a specialized monooxygenase and methyltransferase domain and provide insight into their activity, mechanism and specificity. Our findings expand the catalytic capabilities of trans‐AT PKSs and identify potential strategies for the production of novel oximidine analogues.
Identification of Distinct Genetic Profiles of Palindromic Rheumatism Using Whole‐Exome Sequencing
Authors: Zheng Chenxiang , Wang Fan , Sun Yue , Zhou Zhuochao , You Yijun , He Dongyi , Zhu Xiaoxia , Jiang Lindi , Lu Cui , Wu Lijun , Wang Hongzhi , Mei Hanying , Zeng Ting , Zheng Hui , Teng Jialing , Liu Honglei , Cheng Xiaobing , Su Yutong , Shi Hui , Hu Qiongyi , Jian Xueming , Fahira Aamir , Yang Qiangzhen , Wang Ke , Wen Yanqin , Wang Zhuo , Huang Jinyan , Yang Chengde , Shi Yongyong , Ye Junna 
Published: May 2023

ObjectivePrevious studies have underlined the genetic susceptibility in the pathogenesis of palindromic rheumatism (PR), but the known PR loci only partially explain the disease's genetic background. We aimed to genetically identify PR by whole‐exome sequencing (WES).MethodsThis multicenter prospective study was conducted in 10 Chinese specialized rheumatology centers between September 2015 and January 2020. WES was performed in 185 patients with PR and in 272 healthy controls. PR patients were divided into PR subgroups who were negative for anti–citrullinated protein antibody (ACPA−) and positive for ACPA (ACPA+) according to ACPA titer (cutoff value 20 IU/liter). We conducted whole‐exome association analysis for the WES data. We used HLA imputation to type HLA genes. In addition, we used the polygenic risk score to measure the genetic correlations between PR and rheumatoid arthritis (RA) and the genetic correlations between ACPA− PR and ACPA+ PR.ResultsAmong 185 patients with PR enrolled in our study, 50 patients (27.02%) were ACPA+ and 135 PR patients (72.98%) were ACPA−. We identified 8 novel loci (in the ACPA− PR group: ZNF503, RPS6KL1, HOMER3, HLA–DRA; in the ACPA+ PR group: RPS6KL1, TNPO2, WASH2P, FANK1) and 3 HLA alleles (in the ACPA− PR group: HLA–DRB1*0803 and HLA–DQB1; in the ACPA+ PR group: HLA–DPA1*0401) that were associated with PR and that surpassed genome‐wide significance (P < 5 × 10−8). Furthermore, polygenic risk score analysis showed that PR and RA were not similar (R2 < 0.025), whereas ACPA+ PR and ACPA− PR showed a moderate genetic correlation (0.38 < R2 < 0.8).ConclusionThis study demonstrated the distinct genetic background between ACPA− and ACPA+ PR patients. Additionally, our findings strengthened that PR and RA were not genetically similar.