bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sleep disturbances and their correlation with cardiovascular risk, obesity, and mood disorders in people with HIV
Authors: Mazzitelli Maria , Trunfio Mattia , Milinkovic Ana , Castelli Eleonora , Sasset Lolita , Leoni Davide , Salvucci Margherita , Cazzaro Riccardo , Calcinoni Ilaria , Balducci Pietro , Ribeiro Gustavo Coelho Quirino , Filagrana Giacomo , Scaglione Vincenzo , Cattelan Anna M. 
Published: February 2023

Background:
The relationship between sleep disorders (SDs), cardiovascular risk (CVR), and mood disorders (MDs) has been studied in detail in the general population, but far less in people with HIV (PWH).

Methods:
Cross-sectional analysis in single centre cohort of PWH. Sleep quality was assessed using by Epworth Sleepiness Scale (ESS), Insomnia Severity Index (ISI), Berlin Questionnaire (BQ), Pittsburgh Sleep Quality Index (PSQI); anxiety and depression were evaluated by the Generalized Anxiety Disorder-7 and Patient Health Questionnaire-9. Demographic, clinical and HIV-related data were collected, and Framingham and Data collection on Adverse effects of anti-HIV Drugs (DAD)-10 scores were computed in modelling associations with each SDs scale.

Results:
Data were collected for 721 PWH on stable combination antiretroviral therapy (cART) (median age of 53 years, 71.8% males, 96% with undetectable HIV RNA, 50.3% on cART potentially affecting sleep, and 20.4% on hypno-inducing drugs), 76.9% had SDs 60.3, 31.3, 31.1, and 7.9% at PSQI, BQ, ISI, and ESS, respectively. Anxiety and depression were detected in 28.3 and 16.1% participants, respectively. BQ score was independently associated with high BMI (P < 0.001), Framingham risk >10% (P < 0.001), and both DAD-10R and -10F score >10% (P < 0.001 and P = 0.031). PSQI and ISI scores were independently associated with depression and anxiety (P < 0.001). No association between SDs and specific antiretroviral regimens, nor HIV-related parameters was detected.

Conclusions:
In our cohort of PWH on stable ART, despite the alarmingly higher prevalence, SDs were associated with the same determinants (cardiovascular risk factors and MDs) observed in the general population.
Brief Report: Suboptimal Lopinavir Exposure in Infants on Rifampicin Treatment Receiving Double-dosed or Semisuperboosted Lopinavir/Ritonavir: Time for a Change
Authors: Jacobs Tom G. , Mumbiro Vivian , Chitsamatanga Moses , Namuziya Natasha , Passanduca Alfeu , Domínguez-Rodríguez Sara , Tagarro Alfredo , Nathoo Kusum J. , Nduna Bwendo , Ballesteros Alvaro , Madrid Lola , Mujuru Hilda A. , Chabala Chishala , Buck W. Chris , Rojo Pablo , Burger David M. , Moraleda Cinta , Colbers Angela 
Published: February 2023

Background:
Although super-boosted lopinavir/ritonavir (LPV/r; ratio 4:4 instead of 4:1) is recommended for infants living with HIV and receiving concomitant rifampicin, in clinical practice, many different LPV/r dosing strategies are applied due to poor availability of pediatric separate ritonavir formulations needed to superboost. We evaluated LPV pharmacokinetics in infants with HIV receiving LPV/r dosed according to local guidelines in various sub-Saharan African countries with or without rifampicin-based tuberculosis (TB) treatment.

Methods:
This was a 2-arm pharmacokinetic substudy nested within the EMPIRICAL trial (#NCT03915366). Infants aged 1–12 months recruited into the main study were administered LPV/r according to local guidelines and drug availability either with or without rifampicin-based TB treatment; during rifampicin cotreatment, they received double-dosed (ratio 8:2) or semisuperboosted LPV/r (adding a ritonavir 100 mg crushed tablet to the evening LPV/r dose). Six blood samples were taken over 12 hours after intake of LPV/r.

Results:
In total, 14/16 included infants had evaluable pharmacokinetic curves; 9/14 had rifampicin cotreatment (5 received double-dosed and 4 semisuperboosted LPV/r). The median (IQR) age was 6.4 months (5.4–9.8), weight 6.0 kg (5.2–6.8), and 10/14 were male. Of those receiving rifampicin, 6/9 infants (67%) had LPV Ctrough <1.0 mg/L compared with 1/5 (20%) in the control arm. LPV apparent oral clearance was 3.3-fold higher for infants receiving rifampicin.

Conclusion:
Double-dosed or semisuperboosted LPV/r for infants aged 1–12 months receiving rifampicin resulted in substantial proportions of subtherapeutic LPV levels. There is an urgent need for data on alternative antiretroviral regimens in infants with HIV/TB coinfection, including twice-daily dolutegravir.
Inguinal Sentinel Lymph Node in a Patient With Recurrent Breast Cancer
Authors: Álvarez-Moreno Marta , Álvarez-Santos Juan , Manzarbeitia-Arroba Begoña , Balsa-Bretón María Ángeles , Sandoval-Moreno Cristina 
Published: February 2023

Abstract
Sentinel lymph node biopsy is the standard of care for axillary staging in early, clinically node-negative breast cancer. The lymphatic pathways could be altered after receiving axillary treatments such as radiation and/or axillary lymph node dissection. We report a case of inguinal and contralateral axilla SLNs in breast cancer recurrence.
Bilateral Lymphadenopathy After COVID Vaccine in 18F-Choline PET/MRI Performed for Hyperparathyroidism
Authors: Kibar Ali , Aghazada Fuad , Asa Sertac , Uslu Besli Rabia Lebriz , Sonmezoglu Kerim 
Published: February 2023

Abstract
We describe a case of a 56-year-old woman with primary hyperparathyroidism. 18F-Choline PET/MRI revealed incidental bilateral axillary lymphadenopathy with mild-moderate increased 18F-choline uptake. The patient had her first and third doses of COVID-19 vaccines from the left arm and second dose of vaccine from the right arm before PET examination.
Application of Various Techniques to Gain Insights Into the Complex Urinary Tract Microbial Communities of Renal Transplant Recipients
Authors: Sathiananthamoorthy Sanchutha , Florman Katia , Richard Damien , Cheng Ka Kei , Torri Vittoria , McCaig Fiona , Harber Mark , Rohn Jennifer L. 
Published: February 2023

Background.
Urinary tract infections (UTIs) are prevalent in renal transplant (RTX) recipients and associated with worse outcomes. Early detection by sensitive diagnostic tests and appropriate treatment strategies in this cohort is therefore crucial, but evidence has shown that current methods may miss genuine infections. Research has shed light on the urinary tract microbial ecology of healthy individuals and nontransplant patients with UTI, but information on the RTx cohort is scant. We conducted a cross-sectional study to (i) compare the gold standard diagnostic culture with alternative techniques and (ii) characterize RTx patient urinary microbial communities.

Methods.
Midstream urine specimens were collected from 51 RTx patients attending a renal transplant clinic and 27 asymptomatic controls. Urinary microscopy, dipstick, and routine culture were performed. To improve sensitivity of microbial detection, we cultured the urinary cell sediment and performed 16S rRNA gene sequencing on urine. Uroplakin-positive urothelial cells shed in urine were analyzed by immunofluorescence staining for any bacterial association.

Results.
Sediment culture and 16S rRNA sequencing confirmed detection deficiencies of diagnostic culture and revealed differences in the urobiomes of RTx patients and controls. Specifically, Gardnerella, Escherichia, and Lactobacillus were most abundant in patients, whereas Lactobacillus, Streptococcus, and Gardnerella were most abundant in controls. The application of both culture and sequencing provided a more nuanced view of the urinary microbial communities.

Conclusions.
This study provides insight into the potential problems of diagnostic culture within RTx patients and sheds light on their urinary microbial inhabitants. Further work may identify key microbial signatures and facilitate the development of better tools for UTI detection within this cohort, which could allow targeted intervention before an infection leads to serious consequences.

http://links.lww.com/TXD/A479
Treatment of De Novo Renal Transplant Recipients With Calcineurin Inhibitor–free, Belatacept Plus Everolimus–based Immunosuppression
Authors: Peddi V. Ram , Marder Bradley , Gaite Luis , Oberholzer Jose , Goldberg Ryan , Pearson Thomas , Yang Harold , Allamassey Lisa , Polinsky Martin , Formica Richard N. 
Published: February 2023

Background.
Compared with calcineurin inhibitor–based immunosuppression, belatacept (BELA)-based treatment has been associated with better renal function but higher acute rejection rates. This phase 2 study (NCT02137239) compared the antirejection efficacy of BELA plus everolimus (EVL) with tacrolimus (TAC) plus mycophenolate mofetil (MMF), each following lymphocyte-depleting induction and rapid corticosteroid withdrawal.

Methods.
Patients who were de novo renal transplant recipients seropositive for Epstein-Barr virus were randomized to receive BELA+EVL or TAC+MMF maintenance therapy after rabbit antithymocyte globulin induction and up to 7 d of corticosteroids. The primary endpoint was the rate of biopsy-proven acute rejection at month 6.

Results.
Because of an unanticipated BELA supply constraint, enrollment was prematurely terminated at 68 patients, of whom 58 were randomized and transplanted (intention-to-treat [ITT] population: n = 26, BELA+EVL; n = 32, TAC+MMF). However, 25 patients received BELA+EVL‚ and 33 received TAC+MMF (modified ITT population). In the ITT population, the 6-mo biopsy-proven acute rejection rates were 7.7% versus 9.4% in the BELA+EVL versus TAC+MMF group. The corresponding 24-mo biopsy-proven acute rejection rates were 19.2% versus 12.5% in the ITT population and 16.0% versus 15.2% in the mITT population; all events were Banff severity grade ≤IIA and similar between groups. One patient in each group experienced graft loss unrelated to acute rejection. The 24-mo mean unadjusted estimated glomerular filtration rates were 71.8 versus 68.7 mL/min/1.73 m2 in the BELA+EVL versus TAC+MMF groups. Posttransplant lymphoproliferative disorder was reported for 1 patient in each group. No deaths or unexpected adverse events were observed.

Conclusions.
A steroid-free maintenance regimen of BELA+EVL may be associated with biopsy-proven acute rejection rates comparable to TAC+MMF.
In VitroValidation of Computationally Predicted Oncogenic Driver Mutations in EGFR Tyrosine Kinase Domain
Authors: Warrier Vidya P , Paruchuri Anoosha , Ramasamy Sakthivel , Gromiha Michael M , Karunagaran Devarajan 
Published: February 2023

AbstractEpidermal Growth Factor Receptor (EGFR) signaling is known to play essential roles in growth and development; nevertheless, overexpression and mutation of EGFR have been reported in several cancers. Non-small cell lung cancer (NSCLC), the most observed type of lung cancer, harbors the highest number of EGFR tyrosine kinase mutations and therefore, EGFR has become an important therapeutic target for treatment of these tumors. Tyrosine Kinase Inhibitors (TKIs) are found to be effective in patients whose tumors contain activating mutations in the tyrosine kinase region of the receptor. This would seem to be beneficial in the treatment of EGFR mutation-positive NSCLC patients but the activating mutations should be sensitive to TKIs. Earlier, a machine learning approach was developed to classify single amino acid polymorphisms (SAPs) in EGFR into driver (cancer-causing) and passenger (neutral) mutations using structural and functional features (Anooshaet al., 2015). This study screened all possible point mutations in EGFR and predicted a list of mutations with high probability of being a driver or a passenger. From this list, we selected 2 mutations (G729E and G719F) with high evolutionary conservation score forin vitrovalidation. If proven to be oncogenic drivers and sensitive to EGFR TKIs, these mutations can aid in the early diagnosis and successful therapy of EGFR mutation-positive NSCLC.
Metagenomic analysis of Pigs’ faecal microbiome and its functional response associated with dietary fibre
Authors: Barman Keshab , Choudhury Manasee , Banik Santanu , Pegu Seema Rani , Kumar Sunil , Deb Rajib , Rahman JaizIsfaqure , Rajkhowa Swaraj , Jat Shankar Lal , Rakshit Sujay , Gupta Vivek Kumar , Das Pranab Jyoti 
Published: February 2023

ABSTRACTPig husbandry is the most valued and economically sustainable husbandry in the livestock farming system. The performance and productivity of the pig are chiefly dependent on the nutritional factor steered by gut beneficial bacteria. The swine gut microbiome has a direct relationship with feed efficiency. As a consequence, identifying microbial taxonomy and functional capacity is critical for proper nutrient digestion. In the present investigation, eighteen grower pigs aged 3 months and weighing 30±0.55 kg were allocated into three different groups using a randomized block layout and supplemented with QPM maize fodder of 0, 5, and 10% to the basal diet by substituting (wt/wt on DM) the maize grains, and named as T0, T1, and T2to probe the increase in gut beneficial bacteria found in the faecal contents. For comparison with T0, T1, and T2, a random faecal sample designated R was collected from six grower pigs of the same age group and bodyweight fed a maize-soya bean-based diet. The experiment is being carried out to investigate the effect of various maize fodder levels on the metagenomic profiles of pig gut microbiota using the 16S rRNA gene. All of the experimental diets were iso-nitrogenous, with protein content ranging from 18.37 to 18.63 per cent. The data generated by 16S rRNA amplicon analysis was 68, 56, 61, and 39 Mb in R, T0, T1, and T2 samples, respectively. From taxonomic distribution, bacterial phyla namelyFirmicutes, Bacteroidetes, Proteobacteria, andSpirochetesare found in descending order of relative abundance in the R, T0, and T1 groups, respectively, whileSpirochaetes, Proteobacteria, Fibrobacteres, Bacteroidetes, andFirmicutesare found in descending order of relative abundance in the T2 group. The relative profusion ofFibrobacter succinogenesis 0.89% and 16.84% in the T1 and T2 groups. According to the findings, a higher level of maize fodder in the diet of grower pigs promotes the growth of fibre-degrading bacteria in the gut microbiota, particularlyFibrobacter succinogenes. Moreover, feeding green maize has decreased the population of methanobacteria in the gut of pigs, which in turn has limited the production of methane.
Lateral and longitudinal compaction of PRC1 overlap zones drive stabilization of interzonal microtubules
Authors: Fermino do Rosário Carline , Zhang Ying , Stadnicki Jennifer , Ross Jennifer L. , Wadsworth Patricia 
Published: February 2023

AbstractDuring anaphase, antiparallel overlapping midzone microtubules elongate and form bundles, contributing to chromosome segregation and the location of contractile ring formation. Midzone microtubules are dynamic in early but not late anaphase; however, the kinetics and mechanisms of stabilization are incompletely understood. Using photoactivation of cells expressing PA-EGF-α-tubulin we find that immediately after anaphase onset, a single highly dynamic population of midzone microtubules is present; as anaphase progresses, both dynamic and stable populations of midzone microtubules coexist. By midcytokinesis, only static, non-dynamic microtubules are detected. The velocity of microtubule sliding also decreases as anaphase progresses, becoming undetectable by late anaphase. Following depletion of PRC1, midzone microtubules remain highly dynamic in anaphase and fail to form static arrays in telophase despite furrowing. Cells depleted of Kif4a contain elongated zones of PRC1 and fail to form static arrays in telophase. Cells blocked in cytokinesis form short PRC1 overlap zones that do not coalesce laterally; these cells also fail to form static arrays in telophase. Together, our results demonstrate that dynamic turnover and sliding of midzone microtubules is gradually reduced during anaphase and that the final transition to a static array in telophase requires both lateral and longitudinal compaction of PRC1 containing overlap zones.
Outcome ofDrosophilamicrobiota manipulation depends on dietary preservative formula and batch variation in dietary yeast
Authors: Sannino David R. , Dobson Adam J. 
Published: February 2023

AbstractGut microbiota are fundamentally important for healthy function in their animal hosts. The flyDrosophila melanogasteris a powerful system for understanding the underlying host/microbe interactions, with modulation of the microbiota inducing phenotypic changes that are conserved across animal taxa. The context-dependence of these responses has not been explored systematically, which may confound repeatability. Here we show that the microbiota’s impact on fly triacylglyceride (TAG) levels - a commonly-measured metabolic index - depends on factors in fly media that are rarely considered or controlled, and are not standardized among laboratories: media preservative formula, yeast batch, and the interactive effect of their combinatorial variation. In studies of conventional, axenic and gnotobiotic flies, we found that microbial impacts were apparent only on specific yeast-by-preservative conditions, with TAG levels determined by a tripartite interaction of the three experimental factors. When comparing axenic and conventional flies, we found that preservatives rather than microbiota status was the main driver of variance in host TAG, and certain yeast-preservative combinations reversed microbiota effects on TAG levels. Further, comparisons between TAG levels of axenic flies and those associated withAcetobacter pomorumorLevilactobacillus brevisdetermined that preservatives, microbiota status, and their interaction were the major drivers of TAG variation. Our results suggest that the microbiota shapes the host TAG response in a manner dependent on the combination of the dietary factors of preservative formulation and yeast batch, with implications for repeatability, interpretation, and optimal experimental standards.ImportanceDrosophila melanogasteris a premier model for microbiome science, which has greatly enhanced our understanding of the basic biology of host-microbe biology. However, often overlooked factors such as dietary composition, including yeast batch variability and preservative formulation used, may cofound data interpretation of experiments within the same lab and lead to different findings when comparing between labs. Our study supports this concept; we find that host TAG levels are not solely dependent on the presence or absence of microbiota members, but rather the combinatorial effects of microbiota members, yeast batch, and preservative formulation used, with preservatives being the largest driver. It serves as a cautionary tale that underappreciated components of fly rearing can mask or drive phenotypes that are believed to be impacted by microbiota members.
Open innovation, enterprise value and the mediating effect of ESG
Authors: Luan XiangYu , Wang XiaoHong 
Published: February 2023

PurposeThis paper aims to investigate the impact of open innovation (OI) in Chinese enterprises on enterprise value (EV). At the same time, this research explores the effects of OI in environmental, social and governance (ESG) and also reveals the intermediary role of ESG in the impact of OI on improving EV.Design/methodology/approachThis study builds a theoretical framework to define a set of hypotheses verified in empirical research. Based on the panel data of Chinese listed companies removing missing data from 2011 to 2020, a two-way fixed effect model is used to study the relationship between OI, ESG and EV.FindingsThe research shows that OI of enterprises has effectively improved their value. At the same time, OI can also improve the ESG of enterprises. That OI increases EV is partly by the mediating path of ESG.Originality/valueThis study determines the influence of OI on EV from a stakeholder and sustainable development perspective, clarifying the mediating pathway of ESG in the relationship. Doing so could inspire companies to improve their OI and desire to enhance competitiveness. It will also prompt enterprises to pay attention to social responsibility activities.
A cognitive BPM theory for knowledge-intensive processes
Authors: de Almeida Rodrigues Gonçalves João Carlos , Baião Fernanda Araujo , Santoro Flavia Maria , Guizzardi Giancarlo 
Published: February 2023

PurposeA literature review was conducted in order to establish a detailed definition of a knowledge-intensive process (KiP). Moreover, relevant theories from humanities – especially the fields of linguistics and philosophy – were thoroughly researched and adapted for establishing descriptive and analytical foundations for the phenomena involved. Finally, a cognitive business process management (BPM) theory was proposed in order to assess how sufficient are its respective explanatory and predictive powers. This study aims to discuss the aforementioned objective.Design/methodology/approachThis study proposes a novel theory for KiPs that describe the process flow based on the participants' interactions and their beliefs, desires and intentions as the main drivers of the process enactment and execution.FindingsThe proposal puts forth a comprehensive definition of a KiP, depicting knowledge intensity, participant interaction and decision-making. The inner dynamics of each of these two elements (as well as other associated elements) are described as an information systems (IS) theory that enables the study of KiPs in detail, going beyond the typical techniques of the BPM field and common obstacles.Originality/valueA theory proposal for KiP that applies concepts from speech act theory and intentional states as the main drivers for understanding the process dynamics are, to the best of the authors' knowledge, not present at the literature. Being an original proposal, the real-world scenario discussed brings up the explanatory and predictive powers of the theory as well as its innovative value for research in the field.
iScotland: building a unified model of activism in multi-platform communication environments from traditional PR theory
Authors: Diers-Lawson Audra 
Published: February 2023

PurposeIn recent years, there has been a growth in research aimed at understanding the foundations of modern activist communication in media-rich and multi-platform environments. For example, Chon and Park's analysis of the American Black Lives Matter (BLM) movement built on Kim and Grunig's STOPS model. Yet, social and political injustice can exist for extended periods of time without successful movements emerging, so what leads people to demand social and political change through activism? This paper posits that crisis is trigger that motivates people to activism and evaluates that within the context of the Scottish independence movement.Design/methodology/approachThe study is based on in-depth interviews with 26 advocates for Scottish independence, which yielded more than 32 h of data. Data were analysed using Strauss and Corbin's (1990) constant comparative method approach using open coding, axial coding and selective coding finding thematic saturation after only 10 interviews.FindingsThe study provides a clear extension of Chon and Park's model of activism by finding that crises are critical triggers for activism. Moreover, these findings also provide insights into not only the Scottish independence movement but more broadly the extension of traditional public relations and communication theory in multi-platform and multi-actor environments.Originality/valueThere are several contributions this piece makes. First, this paper extends activist, crisis and strategic communication research to more systematically consider the role that crisis plays in social and political advocacy. Second, this paper affords the opportunity to consider the challenges of communication, democracy and activism in the social media age. Finally, this paper supports an international view that discrimination and affective injustice experiences cut across many different kinds of identities and experiences instead of the traditionally considered ethnic, religious and gender-based experiences traditionally addressed.