bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reducing oxidative protein folding alleviates senescence by minimizing ER‐to‐nucleus H2O2 release
Authors: Cheng Fang , Ji Qianzhao , Wang Lu , Wang Chih‐chen , Liu Guang‐Hui , Wang Lei 
Published: June 2023

AbstractOxidative protein folding occurs in the endoplasmic reticulum (ER) to generate disulfide bonds, and the by‐product is hydrogen peroxide (H2O2). However, the relationship between oxidative protein folding and senescence remains uncharacterized. Here, we find that the protein disulfide isomerase (PDI), a key oxidoreductase that catalyzes oxidative protein folding, accumulated in aged human mesenchymal stem cells (hMSCs) and deletion of PDI alleviated hMSCs senescence. Mechanistically, knocking out PDI slows the rate of oxidative protein folding and decreases the leakage of ER‐derived H2O2 into the nucleus, thereby decreasing the expression of SERPINE1, which was identified as a key driver of cell senescence. Furthermore, we show that depletion of PDI alleviated senescence in various cell models of aging. Our findings reveal a previously unrecognized role of oxidative protein folding in promoting cell aging, providing a potential target for aging and aging‐related disease intervention.
An AlphaFold2 map of the 53BP1 pathway identifies a direct SHLD3–RIF1 interaction critical for shieldin activity
Authors: Sifri Chérine , Hoeg Lisa , Durocher Daniel , Setiaputra Dheva 
Published: June 2023

Abstract53BP1 is a chromatin‐binding protein that promotes DNA double‐strand break repair through the recruitment of downstream effectors including RIF1, shieldin, and CST. The structural basis of the protein–protein interactions within the 53BP1‐RIF1‐shieldin‐CST pathway that are essential for its DNA repair activity is largely unknown. Here, we used AlphaFold2‐Multimer (AF2) to predict all possible pairwise combinations of proteins within this pathway and provide structural models of seven previously characterized interactions. This analysis also predicted an entirely novel binding interface between the HEAT‐repeat domain of RIF1 and the eIF4E‐like domain of SHLD3. Extensive interrogation of this interface through both in vitro pulldown analysis and cellular assays supports the AF2‐predicted model and demonstrates that RIF1‐SHLD3 binding is essential for shieldin recruitment to sites of DNA damage, and for its role in antibody class switch recombination and PARP inhibitor sensitivity. Direct physical interaction between RIF1 and SHLD3 is therefore essential for 53BP1‐RIF1‐shieldin‐CST pathway activity.
Experimentación con carcasas animales para reproducir patrones de marcas de corte: Aplicación a un yacimiento africano de hace dos millones de años
Authors: Domínguez-Rodrigo Manuel 
Published: June 2023

Una vertiente poco explotad de la Arqueogía Experimental es la experimentación con fauna, recreando procesos de intervención antrópica y no antrópica sobre los conjuntos óseos que constituyen los yacimientos arqueológicos. Uno de esos procesos son los patrones de marcas de corte.
Abundance and Characteristics of Microplastics Found in The Gastrointestinal Tract of Commercial Marine Fish from Bitung, North Sulawesi, Indonesia
Authors: Aunurohim Aunurohim , Risawati Eka Krisna , Rahmawati Eka Nur , Ashuri Nova Maulidina , Yalindua Fione Yukita , Ibrahim Putri Saphira , Citrasari Nita , Raufanda Miftakhul Sefti , Prabowo Romanus Edy 
Published: June 2023

Microplastic (MP) pollution is an emerging environmental problem that threatens food security, food safety, and human health since it has been reported to be found in commercial fish consumed by humans. Bitung, North Sulawesi, is one of the biggest contributors to capture fishery production in Indonesia. However, there is no data on microplastic pollution in commercial marine fish from Bitung. Therefore, this research aimed to investigate the presence and identify the visual characteristics (color, shape, size) and the polymer type of microplastics found in the gastrointestinal tract of commercial marine fish from Bitung, North Sulawesi. The gastrointestinal tract was extracted using KOH 10%, and the microplastic was observed under a stereo microscope. A total of 753 microplastic particles were found in the gastrointestinal tract of 74 individuals (prevalence 99%), and there was a statistically significant difference in the abundance of microplastics found in the gastrointestinal tract of pelagic and demersal fish. The average number of microplastic particles found in the gastrointestinal tract of pelagic fish (12,24 ± 2,43) is higher than in demersal fish (7,38 ± 3,48). The dominant color and shape of microplastic found in the gastrointestinal tract of the fish were black and fiber, respectively. At the same time, the dominant microplastic size found in the gastrointestinal tract of demersal fish was bigger (1,001-5,000 µm, 39,4%) compared to pelagic fish (150-500 µm, 47%). The Fourier Transform Spectroscopy (FTIR) analysis result shows that microplastics of the same polymer type can be found in the gastrointestinal tract of both pelagic and demersal fish.
Difusão da inovação na construção civil: um estudo de caso em Ligth Steel Frame
Authors: Haubrich Deise Bitencourt , Gomes Isabel Sparrenberger , Froehlich Cristiane , Nodari Cristine Hermann 
Published: June 2023

Diante da elevada quantidade de recursos consumidos e resíduos gerados pela indústria da construção civil, a busca por sistemas construtivos que levem em consideração os aspectos do desenvolvimento sustentável de forma equilibrada se torna legítima. Novos estudos podem elevar o nível das discussões acerca da disseminação de produtos sustentáveis. O objetivo da pesquisa consiste em verificar através da Teoria da Difusão da Inovação, sob o ponto de vista do empreendedor, em qual situação o sistema construtivo Light Steel Frame se encontra atualmente no mercado. Foi realizado um estudo de caso único em uma empresa situada na Região do Vale do Rio dos Sinos, no Rio Grande do Sul, com foco em construções sustentáveis. Foram realizadas entrevistas semiestruturadas com os gestores da empresa com o intuito de saber, pelo ponto de vista do empreendedor, como o produto está posicionado em relação aos seus consumidores. Os resultados indicam que as construções em Light Steel Frame contemplam as dimensões do tripé da sustentabilidade, e no que se refere a difusão da inovação, se encontram em um intervalo entre os grupos de adotantes iniciais e maioria precoce, intitulado como “abismo”. Ao investigar um produto específico (Light Steel Frame) que faz parte de um mercado importante para a economia do país, sob o prisma de teorias como a da Difusão da Inovação e o Triple Bottom Line, o trabalho fomenta a discussão sobre a sustentabilidade e gera evidências a respeito do status do produto em relação a sua disseminação no mercado local.
A regulação sanitária nos tratados de investimentos brasileiros
Authors: Esis Villarroel Ivette Susana , Achtschin Leonardo Vieira Arruda 
Published: June 2023

A pandemia de Covid-19 tem imposto aos Estados desafios regulatórios em termos de saúde pública, ressoando no direito dos investimentos com o questionamento do tradicional foco na proteção do investidor. A problemática do artigo questiona se o Acordo de Cooperação e Facilitação de Investimentos possui instrumentos normativos garantidores do direito de regular em saúde pública. Para isso, a investigação analisa o trinômio “legitimação-reforço-concretização”. Primeiramente, investiga-se a exceção de saúde pública como legitimação do direito de regular estatal. Depois, investiga-se a responsabilidade social corporativa como reforço desse direito de regular. Por fim, analisa-se o sistema de governança institucional como mecanismo de garantia concreta do direito de regular estatal. O método utilizado é o qualitativo-teórico, a partir de fontes primárias e secundárias. Conclui-se, ao final, que o modelo brasileiro se reveste de potencialidade para promover uma conciliação entre os interesses do investidor e a necessidade do Estado de regular a saúde pública.
Adjacent Segment Disease After Spinal Fusion
Authors: McDonald Christopher L. , Alsoof Daniel , Glueck Jacob , Osorio Camilo , Stone Benjamin , McCluskey Leland , Diebo Bassel G. , Daniels Alan H. , Basques Bryce A. 
Published: June 2023

» Adjacent segment disease is characterized by a degenerative process adjacent to a previously fused spine segment, with new onset of clinical symptoms such as radiculopathy, myelopathy, or instability.» Etiology is related to the natural history of the disease process, increased biomechanical stress at adjacent segments, clinical factors specific to the individual patient, intraoperative factors, and malalignment.» Treatment is usually nonoperative, but surgical intervention can be indicated. Decompression and fusion remain the mainstay of operative treatment, and isolated decompression should be considered in specific cases.» Further randomized controlled trials are needed to establish how the treatment should progress, particularly with the development of minimally invasive and endoscopic surgery.
Wake-induced response of vibro-impacting systems
Authors: Chawla Rohit , Rounak Aasifa , Bose Chandan , Pakrashi Vikram 
Published: June 2023

Abstract
This study investigates the bifurcation behaviour of wake-induced vibro-impacting systems considering
fluid-structure interaction (FSI). The primary structure and the near wake dynamics are
modelled as a harmonic oscillator a Van der Pol oscillator, respectively, and are weakly coupled
to each other. A non-deformable barrier obstructing the motion of the oscillator gives rise
to the impacting dynamics. Instantaneous velocity reversal occurs when the structure impacts
with this barrier. Qualitative changes in the dynamical behaviour of this system are investigated
in the context of discontinuity-induced bifurcations (DIBs) resulting from the interaction
of fluid flow and non-smoothness from the primary structure. Phenomenological behaviour like
co-existence of attractors and period-adding cascades of limit cycles leading to chaos are examined.
Existence of these phenomena are demonstrated via stability analysis using Floquet theory
and associated Lyapunov spectra. A new approach to capture the impact dynamics of the hybrid
system through a transverse discontinuity map is proposed. Orbits obtained using this new algorithm
is demonstrated to accurately predict both stable and chaotic regimes as observed from
the corresponding bifurcation diagrams. The effect of the presence of non-smoothness on the
lock-in phenomenon in systems that involve fluid-structure interaction is studied for the first time.
The Realization of a Digital Atlas of Wetlands using remote sensing and web mapping case of the Oran region
Authors: Faouzi Berrichi , Djemoui Chamakhi , Naima Benshila 
Published: June 2023

Abstract
Wetlands like any other ecosystem have special conditions that differentiate them from other ecosystems. This difference is reflected by the presence of specific plant species related to these biotopes. Among the humid zones of the wilaya of Oran, the great Sebkha, the marshes of the Macta, the Telamine Lake and the saline of Arzew. These sites are known for their fauna and flora biodiversity. This work aims to develop an operational geomatic approach to delineation, evaluation and exploitation of information on the wetlands of the wilaya of Oran. The Digital Atlas made it possible to discover another aspect of this astonishing diversity of wetlands in the Oran region through a new technique for presenting the results exploited under a web mapping. This presentation shows the importance of wetlands in the Oran region that will allow citizens to become acquainted with the richness, beauty and extent of this heritage, will contribute to the awareness raising of the populations and will constitute a valuable instrument of assessment and evaluation available to decision-makers and professionals in nature management and conservation. The results of this study revealed, among other things, that remote sensing research has many scientific achievements of an operational nature. These can potentially be related to managers' need for tools to inventory and delineate wetlands, monitor the evolution and conservation status of wetland habitats. The Digital Atlas made it possible to discover another aspect of this amazing diversity of wetlands of the Oran region through a new technique of presentation of results exploited under a web mapping.
Melt-quenched Carboxylate Metal–Organic Framework Glasses
Authors: Kim Minhyuk , Lee Hwa-Sub , Seo Dong-Hyun , Jeon Eun-chae , Moon Hoi Ri 
Published: June 2023

Abstract
Although carboxylate-based frameworks are commonly used architectures in metal-organic frameworks (MOFs), liquid/glass MOFs have thus far mainly been obtained from azole- or weakly coordinating ligand-based frameworks.1,2 This is because strong coordination bonds of carboxylate ligands to metals block the thermal vitrification pathways of carboxylate-based MOFs.3 In this study, we present the first example of carboxylate-based melt-quenched MOF glasses comprising Mg2+ or Mn2+ and an aliphatic carboxylate ligand, adipate. These MOF have a low melting temperature (Tm) of 284 °C, compared to zeolitic-imidazolate framework (ZIF) glasses,4,5 and superior mechanical properties in terms of hardness and elastic modulus.6 The low Tm is due to the flexibility and low symmetry of the aliphatic carboxylate ligand (raising entropy of fusion (ΔSfus))7,8 and the lack of crystal field stabilization energy on metal ions (reducing enthalpy of fusion (ΔHfus)).9 This research will serve as a cornerstone for the integration of numerous carboxylate-based MOF into MOF glasses.
The Tropical Total Electron Content Enhancement
Authors: Valladares Cesar Enrique , Eccles James Vincent 
Published: June 2023

Abstract
Continuous and regional measurements of total electron content (TEC) over the Americas have revealed that the most significant TEC values during the June solstice frequently reside over Central America. These TEC enhancements are observed during the June solstice afternoon hours, last for a few hours, are constrained to regions of eastward magnetic declination, and occur at magnetic latitudes poleward of 20°. During the December solstice, similar TEC enhancements are seen over the southern coast of Brazil and northern Argentina, also poleward of 20°, but in a region where the declination is westward. Using a physics-based model, it is proposed that the TEC enhancements are produced by an equatorward meridional wind but intensified by the zonal component of the wind, blowing at longitudes where the Earth’s magnetic declination differs from zero. Due to their location near + 23° latitude (tropic of Cancer) in the northern hemisphere and near − 23° latitude (tropic of Capricorn) in the southern hemisphere, the TEC enhancements have been named the tropical TEC enhancement.
Cas13b-mediated RNA targeted therapy alleviates genetic dilated cardiomyopathy in mice
Authors: Li Jiacheng , Kuang Xin , Li Yahuan , Lian Hong , NIE YU 
Published: June 2023

Abstract
Background
Recent advances in gene editing technology have opened up new avenues for in vivo gene therapy, which holds great promise as a potential treatment method for dilated cardiomyopathy (DCM). The CRISPR-Cas13 system has been shown to be an effective tool for knocking down RNA expression in mammalian cells.
Results
In this study, we delivered PspCas13b with hTNNT2R141W gRNA into human TNNT2R141W (hTNNT2R141W) transgenic DCM mouse model using adeno-associated virus (AAV), resulting in effective knockdown of hTNNT2R141W transcripts. Cas13b-mediated knockdown significantly increased myofilament sensitivity to Ca2+, improved cardiac function, and reduced myocardial fibrosis in DCM mice.
Conclusions
These findings suggest that targeting genes through Cas13b represents a promising approach for in vivo gene therapy for genetic diseases caused by aberrant gene expression. Our study provides further evidence of Cas13b's application in genetic disease therapy and paves the way for future applications of genetic therapies for cardiomyopathy.
Better Living with Non-memory Led Dementia: protocol for a feasibility randomised controlled trial of a web-based caregiver educational programme
Authors: Gonzalez Aida Suarez , John Amber , Brotherhood Emilie , Camic Paul ,   Roberta McKee-Jackson1, Melville Mel , Sullivan Mary Pat ,   Rhiannon Tudor-Edwards4, Windle Gill , Crutch Sebastian , Stott Joshua 
Published: June 2023

Abstract

Background:
Non-memory led dementias such as posterior cortical atrophy (PCA), primary progressive aphasia (PPA) and behavioural variant frontotemporal dementia (bvFTD) are low prevalent and often affect individuals under the age of 65. Tailored educational and support resources for caregivers of people living with these dementia phenotypes are scarce and unevenly distributed geographically. Web-based educational programmes are emerging as promising alternatives to improve caregiver self-efficacy and well-being. The aim of this study is to assess the feasibility of a co-produced online educational programme for caregivers of people living PCA, PPA and bvFTD: the Better Living with Non-memory Led Dementia programme.
Methods:
A randomised controlled feasibility trial will be conducted on a sample of 30 caregivers of people living with PCA, PPA and bvFTD. Participants will be recruited among members of the support organisation Rare Dementia Support (based at UCL in the UK). The intervention group will be given access to an 8-week co-produced web-based educational programme consisting of 6 modules addressing education about PCA, PPA and bvFTD, support strategies for the person with dementia, and for the caregiver. The control group will receive treatment as usual (TAU). Feasibility will be measured through feasibility of recruitment, clinical measurement tools and acceptability. Clinical measures will be used to assess preliminary efficacy and data on completion rates, missing data and variability used to decide on measures to be included in a full-scale trial.
Allocation ratio will be 2:1 (intervention: control) stratified by diagnosis. Feasibility of recruitment and acceptability will be assessed. Clinical measures will be administered at baseline and eight weeks and three months post-randomisation. The control group will be offered access to the intervention at the completion of data collection. Participants will be unblinded and all measures will be self-reported online.
Discussion: Online delivered educational programmes show potential for improving care competency of caregivers and may contribute to overcoming geographical inequalities in local provision of support services. This pilot study will inform a fully powered international trial to determine the effectiveness of Better Living with Non-memory led Dementia.
Trial registration: This trial has been registered retrospectively on the Clinical Trials registry on 1st September 2022, registration number NCT05525377.
Preclinical Lymph Node Model for Intraoperative Molecular Imaging of Cancer
Authors: Bou-Samra Patrick , Chang Austin , Arambepola Sachinthani , Guo Emily , Azari Feredun , Kennedy Gregory , Segil Alix , Singhal Sunil 
Published: June 2023

Abstract
Purpose
Lymph node(LN) dissection is part of most oncologic resections. Intraoperatively identifying a positive LN(+ LN), that harbors malignant cells, can be challenging. We hypothesized that intraoperative molecular imaging(IMI) using a cancer-targeted fluorescent prober can identify + LNs. This study aimed to develop a preclinical model of a + LN and test it using an activatable cathepsin-based enzymatic probe, VGT-309.
Procedures
In the first model, we used peripheral blood mononuclear cells (PBMC), representing the lymphocytic composition of the LN, mixed with different concentrations of human lung adenocarcinoma cell line A549. Then, they were embedded in a Matrigel® matrix. A black dye was added to mimic LN anthracosis. Model two was created using a murine spleen, the largest lymphoid organ, injected with various concentrations of A549. To test these models, we co-cultured A549 cells with VGT-309. Mean fluorescence intensity(MFI) was. An independent sample t-test was used to compare the average MFI of each A549:negative control ratio.
Results
A significant difference in MFI from our PBMC control was noted when A549 cells were 25% of the LN (p = 0.046) in both 3D cell aggregate models-where the LNs native parenchyma is replaced and the one where the tumor grows over the native parenchyma. For the anthracitic equivalents of these models, the first significant MFI compared to the control was when A549 cells were 9% of the LN (p = 0.002) in the former model, and 16.7% of the LN (p = 0.033) in the latter. In our spleen model, we first noted significance in MFI when A549 cells were 16.67% of the cellular composition.(p = 0.02)
Conclusions
A + LN model allows for a granular evaluation of different cellular burdens in + LN that can be assessed using IMI. This first exvivo + LN model can be used in preclinical testing of several existing dyes and in creating more sensitive cameras for IMI-guided LN detection.
High-intensity focused ultrasound for endometrial ablation in adenomyosis: A clinical study
Authors: Wu Siyun , Liu Jun , Liu Xiaoshan , Han Yanhua 
Published: June 2023

Abstract

This study aimed to investigate the clinical efficacy of endometrial ablation with high-intensity focused ultrasound (HIFU) for symptom relief in women with adenomyosis. Between July 2014 and July 2020, 167 patients with adenomyosis treated at the Zhongshan City People’s Hospital were enrolled in this study. Patients were divided into two groups according to patient aspirations: the control group, including patients who only underwent ablation of adenomyosis lesions (group A) and the treatment group, including patients who underwent removal of adenomyosis lesions and endometrial ablation (group B). The reduced dysmenorrhea scores (visual analog scale) and menstrual volume scores (pictorial blood assessment chart) were measured before and after treatment. Scores were obtained by subtracting postoperative scores from preoperative scores and were compared to determine whether the symptoms had alleviated. Compared with the menstrual volume of group A, group B showed significant improvements. The average relief rates of dysmenorrhea in the two groups also showed significant improvement. However, the scores in group B showed significant improvement compared with those in group A. Therefore,the findings suggest thatendometrial ablation using HIFU may be superior to conventional therapy for adenomyosis at improving the symptoms of increased menstruation.
Subslab ultra low velocity anomaly uncovered by and facilitating the largest deep earthquake
Authors: Chen Weiwen , Wang Weitao , Wei Shengji 
Published: June 2023

Abstract

It is enigmatic that M8+ earthquakes can take place at depth greater than 600 km inside the slab, where the P-T conditions generally do not favor seismic slip rate (~m/s) on faults. Here we provide new insights to the initial rupture and mechanism of the Mw 8.3 Sea of Okhotsk earthquake by analyzing high-frequency teleseismic array data. We determine the relative location and timing of two early subevents and the geometry and velocity perturbation of a nearby structure anomaly. We found a small-scale (~30x60x60 km) ultralow (-18±2%) P-wave velocity anomaly located beneath the Pacific slab around the 660km discontinuity. The highly melted nature of the anomaly provides significant buoyancy, inducing stress in the slab dramatically closer to the critical condition for thermal runaway weakening that allows the rupture to propagate beyond the metastable olivine wedge, forming M8+ events. Extremely large velocity reduction urges for further mineral physics and geodynamic investigations.