bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Frailty and Cervical Radiographic Parameters on Postoperative Dysphagia Following Anterior Cervical Spine Surgery
Authors: Asada Tomoyuki , Singh Sumedha , Maayan Omri , Shahi Pratyush , Singh Nishtha , Subramanian Tejas , Araghi Kasra , Korsun Maximilian , Tuma Olivia , Pajak Anthony , Lu Amy , Mai Eric , Kim Yeo Eun , Dowdell James , Sheha Evan D. , Iyer Sravisht , Qureshi Sheeraz A. 
Published: September 2023

Study Design.
Retrospective review of a prospectively collected registry.

Objective.
The purpose of the present study was to investigate the impact of frailty and radiographical parameters on postoperative dysphagia after anterior cervical spine surgery (ACSS).

Summary of Background Data.
There is a growing body of literature indicating an association between frailty and increased postoperative complications following various surgeries. However, few studies have investigated the relationship between frailty and postoperative dysphagia after anterior cervical spine surgery.

Materials and Methods.
Patients who underwent anterior cervical spine surgery for the treatment of degenerative cervical pathology were included. Frailty and dysphagia were assessed by the modified Frailty Index-11 (mFI-11) and Eat Assessment Tool 10 (EAT-10), respectively. We also collected clinical demographics and cervical alignment parameters previously reported as risk factors for postoperative dysphagia. Multivariable logistic regression was performed to identify the odds ratio (OR) of postoperative dysphagia at early (2–6 weeks) and late postoperative time points (1–2 years).

Results.
Ninety-five patients who underwent ACSS were included in the study. Postoperative dysphagia occurred in 31 patients (32.6%) at the early postoperative time point. Multivariable logistic regression identified higher mFI-11 score (OR, 4.03; 95% CI: 1.24–13.16; P=0.021), overcorrection of TS–CL after surgery (TS−CL, T1 slope minus C2–C7 lordosis; OR, 0.86; 95% CI: 0.79–0.95; P=0.003), and surgery at C3/C4 (OR, 12.38; 95% CI: 1.41–108.92; P=0.023) as factors associated with postoperative dysphagia.

Conclusions.
Frailty, as assessed by the mFI-11, was significantly associated with postoperative dysphagia after ACSS. Additional factors associated with postoperative dysphagia were overcorrection of TS–CL and surgery at C3/C4. These findings emphasize the importance of assessing frailty and cervical alignment in the decision-making process preceding ACSS.
Construction and Analysis of an LncRNA-miRNA-mRNA Network Based on Competitive Endogenous RNA Reveal miRNAs Potentially Involved in In-stent Restenosis After Percutaneous Coronary Intervention
Authors: Jin Xiao , Wu Bingxin , Han Li , Zhu Xiaofeng 
Published: September 2023

Objective:
Percutaneous coronary intervention (PCI) is one of the most common procedures used for the invasive treatment of patients with coronary heart disease; the incidence of in-stent restenosis (ISR) after PCI is 5% to 15%. In this study, a competitive endogenous RNA (ceRNA) network was constructed to investigate potential mechanisms involved in ISR.

Methods:
The expression data for differentially expressed microRNAs (DEmiRNAs) and messenger RNAs (mRNAs) between patients with and without ISR were obtained using limma package. Long noncoding RNAs (lncRNAs) were predicted based on the DEmiRNAs using the miRDB, miRTarBase, and TargetScan databases. An ISR-specific ceRNA network was subsequently constructed and investigated. To verify the key miRNAs of ceRNA, patients with and without ISR were enrolled from Guangdong Provincial Hospital of Chinese Medicine between January 2017 and December 2018 (n = 8, respectively); plasma was collected from all enrolled patients.

Results:
Based on the raw data obtained from the Gene Expression Omnibus database, 472 DEmiRNAs and 304 differentially expressed messenger RNAs (DEmRNAs) between patients with and without ISR were identified. A ceRNA network was constructed by combining 270 lncRNAs, 3 miRNAs (miR-125, miR-140, and miR-206), and 4 mRNAs (STRADB, TKT, PCTP, and BTG2). The hub genes of the ceRNA network of ISR included the following: miR-125, miR-206, miR-140, PCDHB9, CASC2, BAK1P1, CSPG4P3Y, CSPG4P4Y, STRCP1, and GRIP2. Verification of miRNAs of ceRNA also showed that the expression of miR-206 was upregulated in patients with ISR vs. those without ISR (P < 0.05). In contrast, the expression of miR-140 and miR-125 was downregulated in patients with ISR vs. those without ISR (P < 0.05).

Conclusions:
This study constructed noncoding RNA-related ceRNA networks for ISR. The results indicated that miR-206, miR-125, and miR-140 may be biomarkers of ISR.
Impact of Using a Precompleted Consent Form for Procedural Sedation in the Pediatric Emergency Department
Authors: McCollum Nichole , Silva Olivia , Sigman Laura , Breslin Kristen , Kline Jaclyn 
Published: September 2023

Objectives
This study aimed to compare elements discussed during the consent process for procedural sedation in the pediatric emergency department to documentation and parental recall before and after implementation of a standardized consent form.

Methods
This is a mixed-methods study combining retrospective electronic record review and cross-sectional surveys of providers and parents after consent for procedural sedation. Surveys were obtained before and after implementation of a precompleted consent form. Providers' survey responses were compared with consent documentation. Recall of consent elements discussed by linked parent-provider dyads were compared.

Results
Six hundred fifty-five encounters were reviewed. Pediatric emergency medicine fellows and pediatric emergency department-based pediatricians were more likely to document any benefit (odds ratio [OR], 1.5; 95% confidence interval [CI], 1.0–2.4) or alternative (OR, 2.7; 95% CI, 1.8–3.9) compared with PEM attendings. Providers were more likely to report discussion of failure to complete the procedure (OR, 7.3; 95% CI, 2.3–23.3) and parents were more likely to recall discussion of this risk (OR, 5.3; 95% CI, 1.0–27.8) in the postintervention group. Based on provider recall, using the precompleted consent form was associated with providers discussing at least 2 of the 3 benefits (84.0% vs 97.2%, P < 0.01), 5 of the 5 risks (31% vs 67.7%, P < 0.01), and improved parental recall of risks (5.7% vs 22.9%, P = 0.03). More providers reported taking less than 1 minute to complete the form in the postimplementation group (12.0% vs 43.7%, P < 0.01).

Conclusions
Implementing a precompleted consent form for procedural sedation was associated with providers reporting decreased time spent completing the consent form and better alignment of key consent elements between reported provider discussion and parental recall.
Successful Outcome of Manual Testicular Detorsion Using Point-of-Care Ultrasound Guidance
Authors: Hsu Chien-Te , Chiu Po-Wei 
Published: September 2023

AbstractTesticular torsion is a surgical emergency. It obstructs the blood supply to the testes, leading to testicular ischemia and necrosis. It presents with a sudden onset of severe unilateral testicular pain associated with nausea/vomiting, swollen scrotum, and high-riding testicles with an absent cremasteric reflex and negative Prehn sign. Prompt diagnosis of ischemic testicles using ultrasonography is challenging for emergency physicians. Color Doppler ultrasound may reveal a relative decrease or absence of blood flow in the affected testicle. The most specific ultrasonographic feature was the whirlpool sign of the spermatic cord. Manual detorsion should be performed as soon as possible before surgical intervention. However, manual detorsion may fail because of patient discomfort, incomplete torsion, and rotation of the testicle in a less common direction. We report a case demonstrating ultrasound-guided detorsion in a 14-year-old boy with right testicular torsion. The present case highlights the importance of incorporating ultrasound guidance into manual detorsion, which can improve the success rate of the procedure.
Middle Meningeal Artery Embolization Combined With Endoscopic Treatment for Chronic Subdural Hematoma
Authors: Guo Chong , Zhang Xinfan , Hu Zhongbo , Guo Ke , Li Zefu , Li Jianmin , Peng Jiangtao 
Published: September 2023

Objective:
To explore the clinical feasibility of middle meningeal artery (MMA) embolization combined with endoscopic treatment for new or recurrent chronic subdural hematoma (CSDH).

Methods:
Twenty patients with CSDH treated in the Binzhou Medical University Hospital from June 2020 to October 2022 were analyzed retrospectively. The clinical information, prognosis, imaging results, and surgical results of the patients were collected and analyzed. The authors first performed MMA embolization, and then endoscopic treatment of CSDH was performed after successful embolization of MMA. Results: All 20 patients with CSDH were successfully treated with MMA embolization combined with endoscope-assisted evacuation. The symptoms of all patients were relieved, no surgical complications occurred, and no rebleeding and recurrence were found in follow-up computed tomography.

Conclusion:
Middle meningeal artery embolization combined with endoscopic treatment of CSDH has a good clinical effect, and it may prevent postoperative recurrence.
Monitoring Heart Failure Patients with Mobile Health Technologies: Outcomes from a 180-day Prospective Study
Authors: Mohapatra Sukanya , Issa Mirna , Ivezic Vedrana , Doherty Rose , Marks Stephanie , Lan Esther , Rozett Keith , Cullen Lauren , Reynolds Wren , Rocchio Rose , Fonarow Gregg C. , Ong Michael K. , Speier William F. , Arnold Corey W. 
Published: September 2023

AbstractMobile health (mHealth) methods have grown in popularity in preventative medicine due to their convenience, cost, and ability to acquire actionable data. At the same time, the burden of many diseases has grown due to their prevalence and high rates of morbidity and mortality. To combat this burden, mHealth can be customized to improve adherence to recommended regimens and decrease doctor or hospital visits for patients and their families. Heart failure (HF) is a disease with an especially high burden for which mHealth can be used to promote adherence to advised care plans with the goal of decreasing exacerbations and their associated urgent and emergency care visits. Our study compared adherence to different mHealth monitoring regimens that used activity trackers, scales, and a mobile app with gamification features and a financial incentive. In a prospective analysis of 111 HF patients monitored for 180 days, we found that a regimen including a mobile app with a gamified financial incentive led to significantly higher adherence to activity tracker (95% vs. 72.2%, p=0.0101) and weight (87.5% vs. 69.4%, p=0.0016) monitoring compared to a regimen that included the monitoring devices alone. Our findings indicate that mobile apps with added engagement features can be useful tools to reduce temporal adherence decline and may thus increase the impact of mHealth driven interventions.
Brain map of aging-induced alterations in membrane ganglioside pattern
Authors: Jha Durga , Dovrtelova Gabriela , Telensky Petr , Olkowicz Seweryn , Coufalikova Katerina , Opalka Lukas , Kubicek Karel , Černá Bára , Hajek Roman , Belaskova Silvie , Klanova Jana , Hampl Ales , Damborsky Jiri , Spacil Zdenek 
Published: September 2023

AbstractRecent efforts to develop comprehensive metabolome and proteome brain atlases for animal models have yielded significant progress. However, the ganglioside (GSs) profile of these models remains largely unexplored. As essential components of the brain, GSs play a crucial role in neuronal function. To address this gap in knowledge, we conducted an in-depth analysis of the young and adult rat brain, as well as its major brain regions, using ultra-high performance liquid chromatography and tandem mass spectrometry in positive and negative ion modes. We also analyzed GSs in cerebrospinal fluid (CSF) and serum from matched samples. Our findings indicate a shift in the ratio of a-series to b-series GSs with age, along with region-specific changes accompanied by aging. This could improve our understanding of brain aging and neurodegenerative diseases. Our study complements existing brain atlases of lipidome and protein expression and highlights the importance of further investigating the mechanisms underlying these GSs changes and the potential therapeutic implications of our findings.
Is the neuropeptide PEN a ligand of GPR83?
Authors: Giesecke Yvonne , Asimi Vahid , Stulberg Valentina , Kleinau Gunnar , Scheerer Patrick , Koksch Beate , Grötzinger Carsten 
Published: September 2023

AbstractG protein-coupled receptor 83 (GPR83) is a class A G protein-coupled receptor with predominant expression in the cerebellum and proposed function in the regulation of food intake and in anxiety-like behavior. The neuropeptide PEN has been suggested as a specific GPR83 ligand. However, conflicting reports exist about whether PEN is indeed able to bind and activate GPR83. This study was initiated to evaluate PEN as a potential ligand of GPR83. Employing several second-messenger and other GPCR activation assays as well as a radioligand binding assay, and using multiple GPR83 plasmids and PEN peptides from different sources, no experimental evidence was found to support a role of PEN as a GPR83 ligand.
Immigration delays but does not prevent adaptation following environmental change: experimental evidence
Authors: Durkee Lily F. , Olazcuaga Laure , Melbourne Brett A. , Hufbauer Ruth A. 
Published: September 2023

AbstractIn today’s rapidly changing world, it is critical to examine how animal populations will respond to severe environmental change. Following events such as pollution or deforestation that cause populations to decline, extinction will occur unless populations can adapt in response to natural selection, a process called evolutionary rescue. Theory predicts that immigration can delay extinction and provide novel genetic material that can prevent inbreeding depression and facilitate adaptation. However, when potential source populations have not experienced the new environment before (i.e.,are naive), immigration can counteract selection and constrain adaptation. This study evaluated the effects of immigration of naive individuals on evolutionary rescue using the red flour beetle,Tribolium castaneum,as a model system. Small populations were exposed to a challenging environment, and three immigration rates (zero, one, or five migrants per generation) were implemented with migrants from a benign environment. Following an initial decline in population size across all treatments, populations receiving no immigration gained a higher growth rate one generation earlier than those with immigration, illustrating the constraining effects of immigration on adaptation. After seven generations, a reciprocal transplant experiment found evidence for adaptation regardless of immigration rate. Thus, while the immigration of naive individuals briefly delayed adaptation, it did not increase extinction risk or prevent adaptation following environmental change.
Transcriptomic profiling of Schlemm’s canal cells reveals a lymphatic-biased identity and three major cell states
Authors: Balasubramanian Revathi , Kizhatil Krishnakumar , Li Taibo , Tolman Nicholas , Bhandari Aakriti , Clark Graham , Bupp-Chickering Violet , Kelly Ruth A. , Zhou Sally , Peregrin John , Simón Marina , Montgomery Christa , Stamer W Daniel , Qian Jiang , John Simon W.M. 
Published: September 2023

AbstractSchlemm’s canal (SC) is central in intraocular pressure regulation but requires much characterization. It has distinct inner and outer walls, each composed of Schlemm’s canal endothelial cells (SECs) with different morphologies and functions. Recent transcriptomic studies of the anterior segment added important knowledge, but were limited in power by SEC numbers or did not focus on SC. To gain a more comprehensive understanding of SC biology, we performed bulk RNA sequencing on C57BL/6J SC, blood vessel, and lymphatic endothelial cells from limbal tissue (∼4500 SECs). We also analyzed mouse limbal tissues by single-cell and single-nucleus RNA sequencing (C57BL/6J and 129/Sj strains), successfully sequencing 903 individual SECs. Together, these datasets confirm that SC has molecular characteristics of both blood and lymphatic endothelia with a lymphatic phenotype predominating. SECs are enriched in pathways that regulate cell-cell junction formation pointing to the importance of junctions in determining SC fluid permeability. Importantly, and for the first time, our analyses characterize 3 molecular classes of SECs, molecularly distinguishing inner wall from outer wall SECs and discovering two inner wall cell states that likely result from local environmental differences. Further, and based on ligand and receptor expression patterns, we document key interactions between SECs and cells of the adjacent trabecular meshwork (TM) drainage tissue. Also, we present cell type expression for a collection of human glaucoma genes. These data provide a new molecular foundation that will enable the functional dissection of key homeostatic processes mediated by SECs as well as the development of new glaucoma therapeutics.Significance statementSchlemm’s canal is a critical ocular drainage structure that maintains intraocular pressure with important consequences for glaucoma. Despite this, Schlemm’s canal has been relatively understudied with the molecular process that mediate its function, maintain its health, or that are compromised in glaucoma needing much deeper characterization. This study is important as it provides the most comprehensive molecular characterization of Schlemm’s canal cells to date providing a wealth of molecular details. In addition to highlighting important biological pathways, this information will guide studies to develop new treatments against glaucoma.
Timing-dependent modulation of working memory by VTA dopamine release in medial prefrontal cortex
Authors: Ge Chaofan , Chen Zhaoqin , Sun Fangmiao , Hou Ruiqing , Fan Hongmei , Li Yulong , Li Chengyu T. 
Published: September 2023

SUMMARYDopamine significantly modulates working memory (WM)1–8, a fundamental cognitive function that maintains information during a brief delay period9–11. The temporal precision of dopaminergic modulation in WM and related neural mechanisms remain elusive. Here we unveiled the pivotal role of dopamine timing in WM performance with head-fixed mice engaged in learning an olfactory WM task. Through electrophysiology and optogenetics, we found that dopaminergic neurons in the ventral tegmental area (VTA) could encode WM information during the delay period, and manipulating dopaminergic neuronal activity bidirectionally modulated WM performance in an inverted U-shaped manner. Optogenetic manipulation of VTA activity also induced bidirectional changes in WM-related neural activity in the medial prefrontal cortex (mPFC). Imaging with a dopamine-sensitive GPCR-activation-based sensor (GRABDA)12,13revealed transient dopamine peaks in the mPFC specifically during the early-delay period. Optogenetic stimulation of VTA-to-mPFC dopaminergic projections during the early- and late-delay period enhanced and impaired WM performance, respectively. Manipulations outside these periods or involving the nucleus accumbens had no effect. Single-unit recordings demonstrated that optogenetic excitation of VTA-to-mPFC projections could modulate mPFC neuronal activity in a manner consistent with behavioral modulation. Thus, the timing of dopamine release is critical in modulation of neuronal activity and behavior.
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells
Authors: Torcq Léa , Majello Sara , Vivier Catherine , Schmidt Anne A. 
Published: September 2023

AbstractHematopoietic stem cells emerge in the embryo from an aortic-derived tissue called the hemogenic endothelium (HE). The HE appears to give birth to cells of different nature and fate but the molecular principles underlying this complexity are largely unknown. Here we show, in the zebrafish embryo, that two cell types emerge from the aortic floor with radically different morphodynamics. With the support of live imaging, we bring evidence suggesting that the mechanics underlying the two emergence types rely, or not, on apicobasal polarity establishment. While the first type is characterized by reinforcement of apicobasal polarity and maintenance of the apical/luminal membrane until release, the second type emerges via a dynamic process reminiscent of trans-endothelial migration. Interfering with Runx1 function suggests that the balance between the two emergence types depends on tuning apicobasal polarity at the level of the HE. In support of this and unexpectedly, we show that the zebrafish Pard3 isoform - Pard3ba - is sensitive to interference with Runx1 activity, in aortic endothelial cells. This supports the idea of a signaling cross talk controlling cell polarity and its associated features, between aortic and hemogenic cells. In addition, using new transgenic fish lines that express Junctional Adhesion Molecules and functional interference, we bring evidence for the essential role of ArhGEF11/PDZ-RhoGEF in controlling the HE-endothelial cell dynamic interface, including cell-cell intercalation, which is ultimately required for emergence completion. Overall, we highlight critical cellular and dynamic events of the endothelial-to-hematopoietic transition that support emergence complexity, with a potential impact cell fate.