bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicción de pancreatitis poscolangiopancreatografía retrógrada mediante el uso de niveles séricos de amilasa y lipasa después de 4 horas
Authors: Gálvez Castillo Nicole Anahy , Ochoa Álvarez Michelle Andrea , Palacios Palacios Angely Tatiana , Bosmediano Laverde Mariela Lisbeth , Feijo Erazo Joseph Mateo 
Published: July 2023

Introducción. La predicción temprana de la pancreatitis (PEP) posterior a la colangiopancreatografía retrógrada endoscópica (CPRE) puede permitir el alta segura de los pacientes ambulatorios el mismo día después de la CPRE y un manejo adecuado más temprano. Objetivo. Este estudio tuvo como objetivo evaluar la utilidad de los niveles de amilasa y lipasa sérica 4 horas después de la CPRE para la predicción temprana de PEP e investigar los valores de corte predictivos para los niveles de amilasa y lipasa sérica 4 horas después de la CPRE para el alta segura y el inicio urgente. de reanimación. Metodología. Se revisaron retrospectivamente los datos de 516 pacientes consecutivos con papila nativa que se sometieron a CPRE entre enero de 2020 y agosto de 2022 en el Hospital General Macas. Resultados. Los niveles séricos de amilasa y lipasa se midieron antes y 4 y 24 horas después de la CPRE. PEP ocurrió en 16 (3,1%) pacientes. Valores de p < 0,001). El nivel de amilasa > 1,5 × el límite superior de referencia (ULR) resultó útil para la exclusión de PEP con una sensibilidad del 93,8 %, mientras que 4 × ULR resultó útil para guiar la terapia preventiva con la mejor especificidad del 93,2 %. De manera similar, el nivel de lipasa 2 × ULR mostró la mejor sensibilidad, mientras que 8 × ULR tuvo la mejor especificidad. El análisis de regresión logística mostró que 4 horas después de la CPRE, el nivel de amilasa > 4 × ULR, el nivel de lipasa > 8 × ULR, la esfinterotomía precortada y la esfinterotomía pancreática fueron predictores significativos de PEP. Conclusión. En conclusión, los niveles de amilasa y lipasa 4 horas después de la CPRE son predictores tempranos útiles de PEP que pueden garantizar un alta segura o una reanimación rápida después de la CPRE.
Análisis sobre el reconocimiento legal de la identidad de género en Ecuador. Su necesidad de reforma
Authors: Flores Vera Cristian Andrés , Bustos Gomezcoello Francisco Esaú , Martínez Calderón Karina del Rocío 
Published: July 2023

Introducción: En el Ecuador en el año 2016 entró en vigor la “Ley Orgánica de Gestión de la Identidad y Datos Civiles”, esta ley marco un precedente como un punto de partida para la reivindicación de los derechos humanos del grupo LGBTI; sin embargo, debido al sistema heteronormativo presente en nuestra legislación, hace que esta ley contenga regulaciones que resultan vulneradoras de derechos de estas personas. ya que solo reconoce a las identidades lo que es considerado como normal. Objetivos: En el presente estudio se analizaron las normativas vigentes con la necesidad de realizar una reforma parcial a la Ley Orgánica de Gestión a la Identidad y Datos Civiles del Ecuador, además se analizó la Constitución de la República del Ecuador, Tratados internacionales, Legislación ecuatoriana, Doctrina jurídica en lo que refiere al garantismo jurídico del grupo LGBTI con un estudio comparativo entre la legislación ecuatoriana y argentina en el reconocimiento de la identidad de género del grupo LGBTI. Metodología: La investigación desarrollada comprende un enfoque mixto: cualitativo y cuantitativo, cualitativo basado en la revisión de información, lo cuantitativo se enfocaron en encuestas a los representantes de los grupos LGTBI de la Región Sierra del Ecuador. Finalmente, la profundidad comprende desde un método descriptivo-explicativo, hasta concluir con el inductivo-deductivo. Resultados: Con la tabulación de las encuestas, la lucha de los grupos LGTBI, así como el esfuerzo de los Estados para garantizar su vinculación con la sociedad todavía sigue siendo difícil, es por ello que para pretender disminuir esta brecha de discriminación para marcar un hito en el país ecuatoriano. Conclusiones: Los resultados de este estudio justifican la necesidad de una reforma parcial a la ley orgánica de gestión a la identidad y datos civiles por resultar discriminatoria al momento de reconocimiento del grupo LGBTI. Área de estudio general: Ciencias Sociales. Área de estudio especifico: Derechos humanos.
Impact of Flash Floods On Agri-based Livelihoods In Sylhet Haor Basin
Authors: Akter N , Islam MR , Karim MA , Miah MG , Rahman MM 
Published: July 2023

Bangladesh is most vulnerable to natural disasters and climate change. Over the past few decades, flash floods have frequently affected the livelihood of people in the Sylhet Haor Basin. This study highlights the causes of increased flash floods, their impact on farming practices and livelihoods, and possible solutions to flash flood risk in the Sylhet Haor Basin. Data were collected using a pre-tested structured questionnaire from randomly selected 298 Haor farmers in high and moderately vulnerable flash flood zones across the Haor region during November 2021 and April 2022. In moderately vulnerable areas, the lack of rivers and canal dredging was the main factor contributing to flash flood deterioration. Index scores and ranks revealed that flash floods most severely affect the primary production sector (agriculture) and threaten the lives and livelihoods of the local populace in high- and moderately flood-prone areas. After the severe flash flood, farmers were forced to turn to other occupations instead of agriculture for their livelihood. To reduce the impact of flash floods on particular features or entire wetlands, most farmers preferred the construction of embankments over sandbags, concrete or stone dams, submergible embankments, and rubber dams. For flash flood risk management, farmers in high- and moderately flash flood-vulnerable areas emphasize the construction of higher dams and embankments and the installation of more flood barriers (dams/embankments). The concerned flash flood management agencies could incorporate the key findings of this study while formulating risk mitigation strategies for the Sylhet Haor Basin.
Ann. Bangladesh Agric. (2022) 26 (1) : 61-73
Perhitungan Loss of Load Probability (LOLP) dengan Metode Monte Carlo
Authors: Yuniahastuti Irna Tri 
Published: July 2023

Perhitungan kendalan pada pembangkit PLTU Anggrek 2x25 MW Gorontalo dengan metode monte carlo didapatkan nilai LOLP sebesar nilai 35,55 hari/ tahun dilakukan dengan 2 kali running yaitu 200 sampel dan 500 sampel. Nilai ini masih diatas nilai keandalan standar PLN, hal ini disebabkan pada unit 2 terjadi PO (Planned Outage) dan MO (Maintance Outage) pada bulan Juni-Juli 2021 yaitu berupa first year inspection pada bulan Juli. Nilai FOR (Force Outage Rate) dihitung berdasarkan data gangguan dari pembangkit di tahun 2021. Sedangkan dengan perhitungan manual perhitungan nilai LOLP didapatkan hasil 37,84 hari/ tahun.
Effects of OmniGen® PRO on ruminal fermentation, stress, and inflammation of Holstein cattle induced with a subacute ruminal acidosis
Authors: Jiang Yun , Chapman James D. , Humphrey Brooke , Garcia Miriam 
Published: July 2023

The aim of our study was to examine the effects of an immunomodulatory feed additive (OmniGen® PRO, Phibro Animal Health, Teaneck, NJ) on ruminal fermentation homeostasis and biomarkers of stress and inflammation in dairy heifers following subacute ruminal acidosis (SARA). Holstein heifers (n = 32, 8.4 ± 0.3 months old) were allocated to receive two treatments based on body weight (BW). Treatments were (1) control (CON; n = 16, no additive) and (2) OmniGen® PRO (OGPRO, n = 16, 10 g/100 kg BW, top-dressed). From d 1 to 69, and d 72 to 77, heifers were fed ad libitum a basal TMR formulated for early lactation. On d 70, the TMR offerings were reduced by 50% based on the intake of the previous 3 days. On d 71, heifers were fed ad libitum a starch challenge diet, which was a 50/50 mix steam-rolled barley and the basal TMR on a DM basis, to induce SARA. Rumen fluid and blood samples were collected on d 66, 71, and 73. Prior to the challenge, DMI, ADG, and feed efficiency were not affected by treatment. Following the challenge, all heifers experienced a 43% decline in DMI. Rumen pH was lower on the challenge day than pre and post challenge but was unaffected by treatment. Ruminal lactate was negligible pre and post challenge but increased on the challenge day; OGPRO reduced ruminal lactate compared to CON. At all sampling points, rumen total VFA were higher in OGPRO than in CON. The challenge caused fluctuations in the acetate to propionate ratio in CON, while OGPRO heifers had less variation. Two days post challenge, OGPRO heifers tended to have lower plasma cortisol, haptoglobin, and non-esterified fatty acids (NEFA) than CON heifers. The OGPRO heifers maintained the mean corpuscular hemoglobin concentration (MCHC) and platelet concentration after the challenge, while their levels declined in CON. In this study, supplementing OGPRO to heifers fed an early lactation diet improved rumen fermentation measures prior to the starch challenge and reduced the negative effects of the challenge on rumen fermentation. In addition, following the challenge, indicators of systemic inflammation tended to be lower in heifers supplemented with OGPRO.
Expression and characterization of PrnC—a flavin-dependent halogenase from the pyrrolnitrin biosynthetic pathway of Pseudomonas protegens Pf-5
Authors: Gebauer Jan , Pietruszka Jörg , Classen Thomas 
Published: July 2023

Introduction: The antimicrobial pyrrolnitrin from Pseudomonas strains is formed in four steps from tryptophan and comprises two flavin-dependent halogenases. Both PrnC and PrnA can carry out regioselective chlorination and bromination and are carrier protein-independent. Whilst the tryptophan halogenase PrnA has been studied in detail in the past, this study focuses on the pyrrole halogenating enzyme PrnC.Methods: The halogenating enzyme PrnC, as well as the essential electron suppliers, the flavin reductases, have been produced soluble in E. coli. Furthermore, a screening of a rational compound library revealed that the pyrrole is essential for substrate recognition; however, the substitution pattern of the benzene ring is not limiting the catalysis.Results and discussion: This renders PrnC to be a synthetically valuable enzyme for the synthesis of pyrrolnitrin congeners. For its natural substrate monodechloroaminopyrrolnitrin (MDA), the KM value was determined as 14.4 ± 1.2 µM and a kcat of 1.66 ± 0.02 min−1, which is comparable to other halogenases.
Influence of timing of Levosimendan administration on outcomes in cardiac surgery
Authors: Schiefenhövel Fridtjof , Berger Christian , Penkova Liubov , Grubitzsch Herko , Haller Bernhard , Meyer Alexander , Heringlake Matthias , Sander Michael , Erb Joachim M. , Balzer Felix , Treskatsch Sascha 
Published: July 2023

PurposeThough a subgroup analysis has shown improved survival for patients suffering severely reduced ventricular function undergoing coronary artery bypass grafting, RCTs were not able to demonstrate overall beneficial effects of perioperative Levosimendan in cardiac surgery. This might be due to Levosimendan’s pharmacokinetics reaching a steady-state concentration only 4–8 h after administration. Thus, this study now analysed the influence of timing of Levosimendan administration on perioperative outcome in cardiac surgery patients preoperatively presenting with severely reduced ventricular function and therefore considered at high-risk for intra- or postoperative low cardiac output syndrome. We hypothesized that prolonged preoperative Levosimendan administration (“preconditioning”) would reduce mortality.MethodsAll adult patients undergoing cardiac surgery between 2006 and 2018 perioperatively receiving Levosimendan were included in this retrospective, observational cohort study (n = 498). Patients were stratified into 3 groups: Levosimendan started on the day prior to surgery (“preop”), Levosimendan started on the day of surgery (“intraop”) or post ICU admission (“postop”). After propensity score matching (PSM) was performed, outcomes defined according to proposed standard definitions for perioperative outcome research were compared between groups.ResultsAfter PSM, there were no significant differences in patients’ characteristics, comorbidities and type/priority of surgery between groups. Compared to intraop or postop Levosimendan treatment, preop treated patients had significantly lower in-hospital-mortality (preop vs. intraop. vs. postop = 16,7% vs. 33,3% vs. 42,3%), duration of mechanical ventilation and rate of continuous renal replacement therapy.ConclusionsProlonged preoperative treatment with Levosimendan of cardiac surgery patients preoperatively presenting with severely reduced left ventricular function might be beneficial in terms of postoperative outcome. Our results are in line with recent experts’ recommendations concerning the prolonged perioperative use of Levosimendan. We strongly recommend that future randomized trials include this “preconditioning” treatment as an experimental arm.
The underlying regulatory mechanisms of colorectal carcinoma by combining Vitexin and Aspirin: based on systems biology, molecular docking, molecular dynamics simulation, and in vitro study
Authors: Chen Dengsheng , Chen Ying , Huang Fang , Zhang Xiaoling , Zhou Yulv , Xu Luning 
Published: July 2023

IntroductionColorectal cancer (CRC) is a highly prevalent digestive system malignancy. Aspirin is currently one of the most promising chemopreventive agents for CRC, and the combination of aspirin and natural compounds helps to enhance the anticancer activity of aspirin. Natural flavonoids like vitexin have an anticancer activity focusing on colorectal carcinoma.MethodsThis study investigated the potential mechanism of action of the novel combination of vitexin and aspirin against colorectal cancer through network pharmacology, molecular docking, molecular dynamics simulation, and in vitro experiments.ResultsThe results of network pharmacology suggested that vitexin and aspirin regulate multiple signaling pathways through various target proteins such as NFKB1, PTGS2 (COX-2), MAPK1, MAPK3, and TP53. Cellular experiments revealed that the combined effect of vitexin and aspirin significantly inhibited HT-29 cell growth. Vitexin dose-dependently inhibited COX-2 expression in cells and enhanced the down-regulation of COX-2 and NF-κB expression in colorectal cancer cells by aspirin.DiscussionThis study provides a pharmacodynamic material and theoretical basis for applying agents against colorectal cancer to delay the development of drug resistance and improve the prognosis of cancer patients.
Addressing gross motor function by functional repetitive neuromuscular magnetic stimulation targeting to the gluteal muscles in children with bilateral spastic cerebral palsy: benefits of functional repetitive neuromuscular magnetic stimulation targeting the gluteal muscles
Authors: Grosse Leonie , Späh Malina A. , Börner Corinna , Schnabel Julian F. , Meuche Anne C. , Parzefall Barbara , Breuer Ute , Warken Birgit , Sitzberger Alexandra , Hösl Matthias , Heinen Florian , Berweck Steffen , Schröder Sebastian A. , Bonfert Michaela V. 
Published: July 2023

BackgroundImpaired selective motor control, weakness and spasticity represent the key characteristics of motor disability in the context of bilateral spastic cerebral palsy. Independent walking ability is an important goal and training of the gluteal muscles can improve endurance and gait stability. Combining conventional physical excercises with a neuromodulatory, non-invasive technique like repetitive neuromuscular magnetic stimulation probably enhances effects of the treatment. This prospective study aimed to assess the clinical effects of repetitive neuromuscular magnetic stimulation in combination with a personalized functional physical training offered to children and adolescents with bilateral spastic cerebral palsy.MethodsEight participants Gross Motor Function Classification System level II and III (10.4 ± 2y5m; 50% Gross Motor Function Classification System level II) received a personalized intervention applying functional repetitive neuromuscular magnetic stimulation (12 sessions within 3 weeks; 12,600 total stimuli during each session). At baseline and follow up the following assessments were performed: 10-m-walking-test, 6-min-walking-test, GMFM-66. Six weeks after the end of treatment the patient-reported outcome measure Gait Outcome Assessment List was completed.ResultsGMFM-66 total score improved by 1.4% (p = 0.002), as did scoring in domain D for standing (1.9%,p = 0.109) and domain E for walking, jumping and running (2.6%,p = 0.021). Gait speed or distance walked during 6 min did not improve from baseline to follow up. Patient-reported outcome showed improvement in 4 patients in altogether 14 ratings. Caregiver-reported outcome reported benefits in 3 participants in altogether 10 ratings.ConclusionRepetitive neuromuscular magnetic stimulation promises to be a meaningful, non-invasive treatment approach for children and adolescents with bilateral spastic cerebral palsy that could be offered in a resource-efficient manner to a broad number of patients. To further investigate the promising effects of repetitive neuromuscular magnetic stimulation and its mechanisms of action, larger-scaled, controlled trials are needed as well as comprehensive neurophysiological investigations.
Motor function improvement and acceptability of non-invasive brain stimulation in patients with Parkinson's disease: a Bayesian network analysis
Authors: Qiu Youjia , Yin Ziqian , Wang Menghan , Duan Aojie , Xie Minjia , Wu Jiang , Wang Zhong , Chen Gang 
Published: July 2023

BackgroundParkinson's disease (PD) is a neurodegenerative disorder defined by progressive motor and non-motor symptoms. Currently, the pro-cognitive effects of transcranial direct current stimulation (tDCS) and repetitive transcranial magnetic stimulation (rTMS) are well-supported in previous literatures. However, controversy surrounding the optimal therapeutic target for motor symptom improvement remains.ObjectiveThis network meta-analysis (NMA) was conducted to comprehensively evaluate the optimal strategy to use rTMS and tDCS to improve motor symptoms in PD.MethodsWe searched PubMed, Embase, and Cochrane electronic databases for eligible randomized controlled studies (RCTs). The primary outcome was the changes of Unified Parkinson's Disease Rating Scale (UPDRS) part III score, the secondary outcomes were Time Up and Go Test (TUGT) time, and Freezing of Gait Questionnaire (FOGQ) score. The safety outcome was indicated by device-related adverse events (AEs).ResultWe enrolled 28 studies that investigated various strategies, including high-frequency rTMS (HFrTMS), low-frequency rTMS (LFrTMS), anodal tDCS (AtDCS), AtDCS_ cathode tDCS (CtDCS), HFrTMS_LFrTMS, and Sham control groups. Both HFrTMS (short-term: mean difference (MD) −5.21, 95% credible interval (CrI) −9.26 to −1.23, long-term: MD −4.74, 95% CrI −6.45 to −3.05), and LFrTMS (long-term: MD −4.83, 95% CrI −6.42 to −3.26) were effective in improving UPDRS-III score compared with Sham stimulation. For TUGT time, HFrTMS (short-term: MD −2.04, 95% CrI −3.26 to −0.8, long-term: MD −2.66, 95% CrI −3.55 to −1.77), and AtDCS (short-term: MD −0.8, 95% CrI −1.26 to −0.34, long-term: MD −0.69, 95% CrI −1.31 to −0.08) produced a significant difference compared to Sham stimulation. However, no statistical difference was found in FOGQ score among the various groups. According to the surface under curve ranking area, HFrTMS ranked first in short-term UPDRS-III score (0.77), short-term (0.82), and long-term (0.84) TUGT time, and short-term FOGQ score (0.73). With respect to the safety outcomes, all strategies indicated few and self-limiting AEs.ConclusionHFrTMS may be the optimal non-invasive brain stimulation (NIBS) intervention to improve motor function in patients with PD while NIBS has generally been well tolerated. However, further studies focusing on the clinical outcomes resulting from the different combined schedules of tDCS and rTMS are required.Systematic review registrationhttps://inplasy.com/inplasy-2023-4-0087/, identifier: 202340087.
Burden and trends of stroke attributable to dietary risk factors from 1990 to 2019 in the Belt and Road Initiative countries: an analysis from the global burden of disease study 2019
Authors: Zhang Yue , Luo Zheng , Yi Juan , Zhu Junjie , Qiu Yun , Xu Xiaoyun , Xie Wanying , Wu Jinyi , Lv Huihui , Mou Changhua , Zhang Wei , Li Xiaopan 
Published: July 2023

ObjectivesThis study aimed to compare the burden and trends of stroke attributed to dietary risk factors in the Belt and Road (“B&amp;R”) countries from 1990 to 2019.MethodsThe 2019 Global Burden of Disease (GBD) Study was used to gather information on the burden of stroke attributable to dietary risk factors. Numbers and age-standardized rates (ASRs) of deaths, disability-adjusted life years (DALYs) were determined in 1990 and 2019 among the “B&amp;R” countries. The average annual percent change (AAPC) was used to analyze the temporal trends of diet-induced stroke DALYs from 1990 to 2019 and in the final decade (2010–2019) by Joinpoint regression analysis.ResultsIn 2019, the absolute number of stroke deaths and DALYs attributable to dietary risk factors were 671,872 cases (95% UI 436,354–937,093) and 1.67 million cases (95% UI 1.15–2.24) in China. We found geographical differences in mortality and DALYs of diet-attributable stroke among member countries, with Bulgaria, Hungary and Serbia being the three highest countries in 1990, Bulgaria, North Macedonia and Montenegro in Central Asia in 2019. The ASRs of diet-induced stroke mortality and DALYs were generally declining in most member states from 1990 to 2019, however, the corresponding metrics in Mongolia remained high. The fastest decline in ASR of mortality and DALYs for diet-induced stroke was seen in Estonia, Eastern Europe, with AAPC values of −7.09% (95%CI: −7.72, −6.46%) and − 6.62% (95%CI: −7.20, −6.03%), respectively. We noted a substantial downward trend in ASR of mortality and DALYs from diet-induced stroke changes in the final decade (2010–2019) for most member states. The ASR of DALYs for diet-induced stroke decreased greater in females than in males. For those aged 50–74, the DALYs for stroke due to dietary risk factors in all other member countries of the “B&amp;R” showed a decreasing trend, except for the Philippines, which rose (AAPC = 2.13, 95%CI: 1.40–2.87%) and Turkmenistan, which remained stable (AAPC = 0.05, 95%CI: −0.43–0.33%).ConclusionThe burden of diet-induced stroke varies substantially across “B&amp;R” countries and threaten public health, relevant evidence-based policies and interventions should be adopted to address the future burden of stroke in “B&amp;R” countries through extensive collaboration.