bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laparoscopic common bile duct exploration with primary closure is preferred for selected elderly individuals with choledocholithiasis
Authors: Luo Tingyi , Huang Yonggang , Wang Shilin , Yang Tingsong , Gong Jian , Zhou Bo , Song Zhenshun , Meng Hongbo , Xu Bin 
Published: March 2023

AbstractBackgroundLaparoscopic common bile duct exploration with primary closure (LCBDE‐PC) exhibits more benefits than other surgeries for patients with choledocholithiasis. It remains unclear whether it is feasible for and beneficial to elderly individuals. This study aimed to clarify and stratify elderly patients who would benefit from LCBDE‐PC.MethodsA retrospective study of 1240 patients with choledocholithiasis who underwent laparoscopic procedures between 2011 and 2019 was conducted. Patients were divided into the young group (<65 years old, n = 708) and the elderly group (≥65 years old, n = 532). Perioperative outcomes were compared between the two groups.ResultsLaparoscopic common bile duct exploration with primary closure was successfully performed in 90.20% of the elderly and 94.20% of the young. No significant differences were observed between the two groups regarding reoperation, postoperative bile leakage, residual stones, drainage removal, and postoperative mortality. Compared with the young, the elderly had longer postoperative hospital stay (p = 0.035) and delayed postoperative eating time (p = 0.036) in the matched cohort. Independent risk factors for failed LCBDE‐PC were preoperative pancreatitis (p = 0.018), year of the surgeon's experience (p = 0.008), preoperative C‐reactive protein level (p = 0.034), preoperative total bilirubin (p = 0.021), impacted common bile duct (CBD) stones (p = 0.006), blood loss (p = 0.001), and edema of the CBD (p = 0.001). A novel nomogram for predicting failed LCBDE‐PC in elderly individuals exhibited a sufficient discriminative ability according to the estimated area under the curve (AUC) of 0.869 (95% CI: 0.817–0.921, p < 0.01).ConclusionLaparoscopic common bile duct exploration with primary closure is safe, feasible, and effective for elderly individuals with choledocholithiasis. Elderly patients with a high risk of failed LCBDE‐PC should be cautious of undergoing LCBDE‐PC.
Conditioning therapy with N‐acetyl‐L‐cysteine, decitabine and modified BUCY regimen for myeloid malignancies patients prior to allogeneic hematopoietic stem cell transplantation
Authors: Tang Yaqiong , Zhang Ziyan , Liu Silu , Yao Yifang , Pan Tingting , Qi Jiaqian , Kang Huizhu , Liu Yuejun , Cai Chengsen , Zhou Meng , He Xuefeng , Hu Xiaohui , Ma Xiao , Wu Depei , Han Yue 
Published: March 2023

AbstractConditioning therapy is an essential procedure prior to hematopoietic stem cell transplant (HSCT), imposing a great impact on the outcomes of recipients. We performed a prospective randomized controlled trial to assess the outcome of HSCT recipients with myeloid malignancies after receiving the conditioning therapy consisting of modified BUCY (mBUCY), N‐acetyl‐L‐cysteine (NAC), and decitabine. Enrolled patients were randomly allocated to either Arm A (decitabine, day −12 to −10; NAC, day −9 to +30; mBUCY, day −9 to −2), or Arm B (mBUCY regimen followed by stem cells infusion). Seventy‐six patients in Arm A and 78 patients in Arm B were finally evaluated. The results showed platelet recovery accelerate in Arm A, with more patients achieving a platelet count of ≥50 × 109/L than Arm B at day +30 and +60 (p = .004 and .043, respectively). The cumulative incidence of relapse is 11.8% (95% CI 0.06–0.22) in Arm A, and 24.4% (95% CI 0.16–0.35) in Arm B (p = .048). The estimated 3‐year overall survival rate was 86.4% (±4.4%) and 79.9% (±4.7%) in 2 arms, respectively (p = .155). EFS at 3 years was 79.2% (±4.9%) in Arm A and 60.0% (±5.9%) in Arm B (p = .007). Intracellular reactive oxygen species (ROS) level was found to be reversely correlated with platelet recovery, and fewer patients in Arm A displayed excessive ROS within hematopoietic progenitor cells compared to Arm B. In conclusion, the addition of decitabine and NAC to mBUCY is a feasible and promising conditioning therapy for myeloid malignancies patients.
Hematocolpos due to lower vaginal agenesis in an adolescent girl
Authors: Imai Kazunori , Aoyama Kohei , Goto Takato , Kitaori Tamao , Iguchi Toshiyuki , Sasano Hiroshi , Hattori Tomonori , Sugiura‐Ogasawara Mayumi , Saitoh Shinji 
Published: March 2023

BackgroundHematocolpos due to imperforate hymen is an important differential diagnosis of abdominal pain in early adolescent stage. However, hematocolpos due to lower vaginal agenesis must be considered because the management differs.Case PresentationA healthy 11‐year‐old girl presented with a 2‐day left lower abdominal pain history. Her breast development had begun, but she had not reached menarche. Computed tomography showed high absorptive value liquid filling the upper vaginal to uterine cavity, a pale highly absorptive fluid component suggestive of hemorrhagic ascites in the abdominal cavity on both sides of the uterus, and normal bilateral ovaries. Magnetic resonance imaging diagnosed hematocolpos due to lower vaginal agenesis. The blood clot was aspirated with a transabdominal ultrasound‐guided transvaginal puncture.ConclusionHistory‐taking, imaging tests, and appropriate collaboration with obstetrician/gynecologist with awareness of secondary sexual characteristics were crucial in this case.
Construction of Multiform Hollow‐Structured Covalent Organic Frameworks via a Facile and Universal Strategy for Enhanced Sonodynamic Cancer Therapy
Authors: Liu Sainan , Dou Kunkun , Liu Bin , Pang Maolin , Ma Ping'an , Lin Jun 
Published: March 2023

AbstractThe special structural morphology of hollow covalent organic frameworks (HCOFs) has an important influence on their applications. However, the rapid and precise control of morphology for HCOFs still remains largely challenging. Herein, we present a facile and universal two‐step strategy based on solvent evaporation and oxidation of imine bond for the controllable synthesis of HCOFs. The strategy enables to prepare HCOFs in a greatly shortened reaction time and seven kinds of HCOFs are fabricated by the oxidation of imine bond via hydroxyl radicals (⋅OH) generated from Fenton reaction. Importantly, a fascinating library of HCOFs with diverse nanostructures, including bowl‐like, yolk‐shell, capsule‐like and flower‐like morphologies, has been ingeniously constructed. Owing to the large cavities, the obtained HCOFs are ideal candidates for drug delivery, which are employed to load five small molecule drugs, achieving the enhanced sonodynamic cancer therapy in vivo.
Behavioral correlates of fascicular organization: The confluence of muscle architectural anatomy and function
Authors: Dickinson Edwin , Hartstone‐Rose Adam 
Published: March 2023

AbstractMuscle is a complex tissue that has been studied on numerous hierarchical levels: from gross descriptions of muscle organization to cellular analyses of fiber profiles. In the middle of this space between organismal and cellular biology lies muscle architecture, the level at which functional correlations between a muscle's internal fiber organization and contractile abilities are explored. In this review, we summarize this relationship, detail recent advances in our understanding of this form‐function paradigm, and highlight the role played byThe Anatomical Recordin advancing our understanding of functional morphology within muscle over the past two decades. In so doing, we honor the legacy of Editor‐in‐Chief Kurt Albertine, whose stewardship of the journal from 2006 through 2020 oversaw the flourishing of myological research, including numerous special issues dedicated to exploring the behavioral correlates of myology across diverse taxa. This legacy has seen theThe Anatomical Recordestablish itself as a preeminent source of myological research, and a true leader within the field of comparative anatomy and functional morphology.
Evaluation of suitable reference genes for expression analysis using quantitative real‐time polymerase chain reaction in the parasitoid Exorista sorbillans (Diptera: Tachinidae)
Authors: Yang Jin , Jiang Zhe , Xu Qian , Liu Xinyi , Dai Minli , Li Bing , Wei Jing 
Published: March 2023

AbstractThe parasitoid Exorista sorbillans (Diptera: Tachinidae) is a larval endoparasitoid of the silkworm Bombyx mori, causing severe damage to silkworm cocoon industry. It is also an important natural enemy resource of insect pests in agriculture and forestry. Despite their roles in biocontrol and pest status on sericulture, there has been limited research on the functional studies of dipteran parasitoids. Quantitative real‐time polymerase chain reaction (qRT‐PCR) is the most commonly used to address gene functions. Using qRT‐PCR, stably expressed reference genes under different experimental conditions are required to normalize the expression of target genes. However, no information regarding suitable qRT‐PCR reference genes in dipteran parasitoids has been reported. In this study, we evaluate the expression stability of nine commonly used reference genes in insects including eukaryotic translation elongation factor 1δ (eEF1δ), elongation factor 2, 18S ribosomal RNA (18S rRNA), tubulin 3, actin87, ribosomal protein 49 (RP49), ribosomal protein S15, glyceraldehyde‐3‐phosphate dehydrogenase, and TATA‐box binding protein (TBP) in E. sorbillans under different treatments, including tissues, developmental stages, genders, feeding density and pesticide stress, using ∆Ct, BestKeeper, geNorm, Normfinder and RefFinder, respectively. The results showed that the genes RP49, eEF1δ and 18S rRNA were recommended as the most suitable reference genes in E. sorbillans across all experimental conditions. This finding provides the necessary foundation for future functional studies in E. sorbillans and its effective use in both sericulture and pest control.
Clinical and genetic analysis of Vietnamese patients diagnosed with early‐onset Parkinson's disease
Authors: Do Minh Duc , Tran Tai Ngoc , Luong An Bac , Le Linh Hoang Gia , Van Le Tuan , Le Khuong Thai , Van Vo Niem Thanh , Le Thuc‐Nhi Nguyen , Vu Hoang Anh , Mai Thao Phuong 
Published: March 2023

AbstractBackgroundGenetic factors play a crucial role in the pathogenesis of Parkinson's disease (PD). However, no comprehensive study has described genetic alterations in Vietnamese patients diagnosed with PD. This study aimed to identify genetic causes and their association with clinical phenotypes in a Vietnamese PD cohort.MethodsA total of 83 patients with early‐onset PD (disease onset before the age of 50) were recruited for genetic analysis using a combination of multiplex ligation‐dependent probe amplification and next‐generation sequencing for a panel of 20 PD‐associated genes.ResultsIt was found that 37 out of 83 patients carried genetic alterations, with 24 pathogenic/likely pathogenic/risk variants and 25 variants of uncertain significance. The pathogenic/likely pathogenic/risk variants were mostly detected in LRRK2, PRKN, and GBA, while the variants of uncertain significance were identified in 12 different genes that were studied. The most common genetic alteration was LRRK2 c.4883G>C (p.Arg1628Pro), and patients with PD carrying this variant were found to have a distinct phenotype. Participants carrying pathogenic/likely pathogenic/risk variants had a significantly higher rate of a family history of PD.ConclusionThese results provide a further understanding of genetic alterations associated with PD in a South‐East Asian population.
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth
Authors: Lam Cynthia , Carver Joseph , Ng Domingos , Zhan Dejin , Tang Danming , Kandamkalam Thara , Snedecor Brad , Barnard Gavin , Shen Amy , Misaghi Shahram 
Published: March 2023

AbstractChinese hamster ovary (CHO) cells are commonly used for the expression of therapeutic proteins. To increase the titer output of CHO production cultures either specific productivity (Qp), growth, or both need to be increased. Generally, Qp and growth are inversely correlated and cell lines with high Qp have slower growth and vice versa. During the cell line development (CLD) process, the faster‐growing cells tend to take over the culture and represent the majority of the isolated clones post single cell cloning. In this study, combinations of regulated and constitutive expression systems were used to supertransfect targeted integration (TI) cell lines expressing the same antibody either constitutively or under‐regulated expression. Clone screening with a hybrid expression system (inducible + constitutive) allowed identification and selection of higher titer clones under uninduced conditions, without a negative impact on cell growth during clone selection and expansion. Induction of the regulated promoter(s) during the production phase increased the Qp without negatively affecting growth, resulting in approximately twofold higher titers (from 3.5 to 6–7 g/L). This was also confirmed using a 2‐site TI host where the gene of interest was expressed inducibly from Site 1 and constitutively from Site 2. Our findings suggest that such a hybrid expression CLD system can be used to increase production titers, providing a novel approach for expression of therapeutic proteins with high titer market demands.
A systematic review of cognitive interventions for adult patients with brain tumours
Authors: Kirkman Matthew A. , Ekert Justyna O. , Hunn Benjamin H. M. , Thomas Michael S. C. , Tolmie Andrew K. 
Published: March 2023

AbstractBackgroundNeurocognitive impairments are common in patients with current or previously treated brain tumours, and such impairments can negatively affect patient outcomes including quality of life and survival. This systematic review aimed to identify and describe interventions used to ameliorate (improve) or prevent cognitive impairments in adults with brain tumours.MethodsWe performed a literature search of the Ovid MEDLINE, PsychINFO and PsycTESTS databases from commencement until September 2021.ResultsIn total, 9998 articles were identified by the search strategy; an additional 14 articles were identified through other sources. Of these, 35 randomised and nonrandomised studies were deemed to meet the inclusion/exclusion criteria of our review and were subsequently included for evaluation. A range of interventions were associated with positive effects on cognition, including pharmacological agents such as memantine, donepezil, methylphenidate, modafinil, ginkgo biloba and shenqi fuzheng, and nonpharmacological interventions such as general and cognitive rehabilitation, working memory training, Goal Management Training, aerobic exercise, virtual reality training combined with computer‐assisted cognitive rehabilitation, hyperbaric oxygen therapy and semantic strategy training. However, most identified studies had a number of methodological limitations and were judged to be at moderate‐to‐high risk of bias. In addition, it remains unclear whether and to what extent the identified interventions lead to durable cognitive benefits after cessation of the intervention.ConclusionThe 35 studies identified in this systematic review have indicated potential cognitive benefits for a number of pharmacological and nonpharmacological interventions in patients with brain tumours. Study limitations were identified and further studies should focus on improved study reporting, methods to reduce bias and minimise participant drop‐out and withdrawal where possible, and consider standardisation of methods and interventions across studies. Greater collaboration between centres could result in larger studies with standardised methods and outcome measures, and should be a focus of future research in the field.
Elemane‐Type Sesquiterpene, Acetonide Derived Polyacetylene and Other Constituents from the Whole Plant of Gymnanthemum theophrastifolium (Schweinf. ex Oliv. & Hiern) H.Rob. and Their Chemophenetic Significance
Authors: Tafouo Tsakou Armelle , Laure Ndontsa Blanche , Carly Nono Nono Eric , Tseme Wandji Nadine , Thierry Mbahbou Bitchagno Gabin , Jules Kezetas Bankeu Jean , Azefack Tapondjou Leon , Sewald Norbert , Ndjakou Lenta Bruno 
Published: March 2023

AbstractThe chemical investigation of the methanol extract of the whole plant of Gymnanthemum theophrastifolium (Schweinf. ex Oliv. & Hiern) H.Rob. (Asteraceae) led to the isolation of a new elemane‐type sesquiterpene (1), a new acetonide derived polyacetylene (2) and a naturally occurring compound (3) from the plant kingdom along with sixteen known compounds (4–19). Their structures were elucidated by extensive NMR and MS analysis. This is the first report on the chemical constituents of G. theophrastifolium. Furthermore, compounds 12, 13, and 14 are reported for the first time from the family Asteraceae, while compound 9 is reported for the first time from the genus Gymnanthemum. Thus, the present results provide valuable insights to the chemophenetic knowledge of G. theophrastifolium, which is also discussed in this work.
Bioorthogonal Peptide Enrichment from Complex Samples Using a Rink‐Amide‐Based Catch‐and‐Release Strategy
Authors: van Leeuwen Tyrza , Doelman Ward , van den Kieboom Robin W. R. , Florea Bogdan I. , van Kasteren Sander I. 
Published: March 2023

AbstractUptake and processing of antigens by antigen presenting cells (APCs) is a key step in the initiation of the adaptive immune response. Studying these processes is complex as the identification of low abundant exogenous antigens from complex cell extracts is difficult. Mass‐spectrometry based proteomics – the ideal analysis tool in this case – requires methods to retrieve such molecules with high efficiency and low background. Here, we present a method for the selective and sensitive enrichment of antigenic peptides from APCs using click‐antigens; antigenic proteins expressed with azidohomoalanine (Aha) in place of methionine residues. We here describe the capture of such antigens using a new covalent method namely, alkynyl functionalized PEG‐based Rink amide resin, that enables capture of click‐antigens via copper‐catalyzed azide‐alkyne [2 + 3] cycloaddition (CuAAC). The covalent nature of the thus formed linkage allows stringent washing to remove a‐specific background material, prior to retrieval peptides by acid‐mediated release. We successfully identified peptides from a tryptic digest of the full APC proteome containing femtomole amounts of Aha‐labelled antigen, making this a promising approach for clean and selective enrichment of rare bioorthogonally modified peptides from complex mixtures.
Green Synthesis of Gold Nanoparticles Using Pithecellobium dulce Leaf Extract and Its Biological Activities
Authors: Vincent Jacob , Lekha N. Chandra 
Published: March 2023

AbstractPithecellobium dulce leaf extract was used to synthesize gold nanoparticles (AuNPs) for the first time. This bio‐synthesized novel material was investigated by UV‐visible spectra, high‐resolution transmission electron microscopy, powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy‐dispersive analysis by X‐ray. Excellent antibacterial activity was revealed against Staphylococcus aureus and Escherichia coli. Significant antifungal activities by the AuNPs were also demonstrated. The analysis of AuNPs by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay proved 100 % toxic effect and the minimum value of half‐maximal inhibitory concentration (IC50) indicates that it is safe to be used as drug in the medical field.
Correlation of Experimental and Calculated Reaction Enthalpies with Ligand Donor Strengths
Authors: Löffler Julian , Vanden Broeck Sofie M. P. , Cazin Catherine S. J. , Nolan Steven P. , Gessner Viktoria H. 
Published: March 2023

AbstractYlide‐functionalized phosphines (YPhos) have recently proven to be strongly donating ligands that enable high catalyst activities in gold(I)‐mediated transformations. We now report on a calorimetric study dealing with the [Au(YPhos)Cl] system and assess YPhos‐Au bond dissociation enthalpies (BDE). Comparison with other commonly used phosphines confirmed the high binding strengths of the YPhos ligands. Furthermore, the values of the reaction enthalpies were shown to correlate with the electronic properties of the ligands measured via the Tolman electronic parameter or the calculated molecular electrostatic potential at phosphorus. Notably, the reaction enthalpies can conveniently be derived by computational methods, thus making these easy‐to‐obtain descriptors for ligand donor property quantification.
Pyridoxine‐dependent epilepsy: Current perspectives and questions for future research
Authors: Coughlin Curtis R. , Gospe Sidney M. 
Published: March 2023

AbstractPyridoxine‐dependent epilepsy (PDE) was historically defined by a dramatic clinical response to a trial of pyridoxine and the re‐emergence of seizures after withdrawal of pyridoxine. Research conducted over the last seven decades has revealed that the phenotype of PDE results from multiple genetic disorders, and the most common disorder, PDE‐ALDH7A1, is caused by a deficiency of an enzyme involved in lysine metabolism. PDE‐ALDH7A1 is characterized by more than epilepsy, as many patients have abnormalities of brain development, and most patients have intellectual and developmental disability. Treatment aimed at the underlying metabolic defect, in addition to pyridoxine supplementation, has improved clinical outcomes. Recently discovered biomarkers and genetic testing allow for the diagnosis of PDE‐ALDH7A1 without the need of a pyridoxine trial and hold the promise for newborn screening. Despite these many advances, PDE‐ALDH7A1 remains a clinical and biochemical conundrum. The increasing use of model systems and an international collaboration of clinician‐scientists are among the reasons to be optimistic that these questions will be answered in the near future and that the clinical outcomes and quality of life will continue to improve for patients with PDE‐ALDH7A1.
Transition Metal‐Catalyzed C−H Functionalization Through Electrocatalysis
Authors: Baroliya Prabhat Kumar , Dhaker Mukesh , Panja Subir , Al‐Thabaiti Shaeel Ahmed , Albukhari Soha M. , Alsulami Qana A. , Dutta Arnab , Maiti Debabrata 
Published: March 2023

AbstractElectrochemically promoted transition metal‐catalyzed C−H functionalization has emerged as a promising area of research over the last few decades. However, development in this field is still at an early stage compared to traditional functionalization reactions using chemical‐based oxidizing agents. Recent reports have shown increased attention on electrochemically promoted metal‐catalyzed C−H functionalization. From the standpoint of sustainability, environmental friendliness, and cost effectiveness, electrochemically promoted oxidation of a metal catalyst offers a mild, efficient, and atom‐economical alternative to traditional chemical oxidants. This Review discusses advances in the field of transition metal‐electrocatalyzed C−H functionalization over the past decade and describes how the unique features of electricity enable metal‐catalyzed C−H functionalization in an economic and sustainable way.