bluuebunny/crossref_metadata_2025_split huggingface dataset:
Persistence of ex vivo expanded tumour and pathogen specific T-cells after allogeneic stem cell transplant for myeloid malignancies (the INTACT study)
Authors: Jiang Wei , Avdic Selmir , Lee Koon , Street Janine , Castellano-González Gloria , Simms Renee , Clancy Leighton , Blennerhassett Richard , Patrick Ellis , Chan Adam , McGuire Helen , Myers Nadav , Gloss Brian , Gabriel Melissa , Bateman Caroline , Micklethwaite Ken , Gottlieb David , Blyth Emily 
Published: June 2023

Abstract
Disease relapse and infections cause significant morbidity and mortality post-allogeneic stem cell transplant (HSCT), which remains the only cure for many myeloid malignancies. We investigated a novel combination of donor-derived, tumour-associated, antigen-specific T-cells targeting Wilm’s tumour 1 (WT1) and preferentially expressed antigen in melanoma (PRAME), and multipathogen T-cells targeting CMV, EBV, Adenovirus and Aspergillus given prophylactically post-HSCT. Ten patients with acute myeloid leukaemia (n=6) or high risk myelodysplasia (n=4) who overexpressed WT1 and/or PRAME on diagnostic tumour samples received 1 infusion of multipathogen and 1-4 infusions of tumour-specific T-cells (all at 2x107cells/m2). There were no infusion-related severe adverse events. Low level viral reactivations occurred (CMV n=5, EBV n=7, Adenovirus n=1), however none required treatment. There were no cases of viral tissue disease or invasive fungal infections. At a median 2 years post-transplant, overall survival was 80%, all surviving patients were in complete remission and 6/8 patients had ECOG 0/1. Acute GVHD occurred in 2/10 patients, chronic GVHD in another 2/10. Infusion was associated with rapid, sustained reconstitution of pathogen- and tumour-specific immunity as measured by MHC tetramer for CMV and T-cell receptor based clone tracking. This novel combination of T-cell therapies was safe and associated with excellent clinical outcomes.
CELL-E: zero-shot sequence-to-image synthesis for protein localization prediction
Authors: Khwaja Emaad , Song Yun , Huang Bo 
Published: June 2023

Abstract
Accurately predicting cellular activities of proteins based on their primary amino acid sequences would greatly improve our understanding of the proteome. In this paper, we present CELL-E, a text-to-image transformer model that generates 2D probability density images describing the spatial distribution of proteins within cells. Given an amino acid sequence and a reference image for cell or nucleus morphology, CELL-E predicts a more refined representation of protein localization, as opposed to previous in silico methods that rely on pre-defined, discrete class annotations of protein localization to subcellular compartments.
The Cursoriality of Triassic Ornithodirans and Their Implications and Inferences for Early Ornithodiran Evolution
Authors: Meyer Gabriel 
Published: June 2023

Abstract
The phylogenetic placement of many Triassic Ornithodirans is convoluted and in need of more data for the best possible placement. Lagerpeptidae and Silesauridae are both under consideration for placement differing than their traditional setting in Dinosauromorpha. The cursoriality of many of these animals has never been assessed, and it could prove useful for further research. Measurements were taken for seventeen taxa from the literature and three types of cursoriality scores were constructed: tibial, third metatarsal, and lower leg length and then analyzed for significance. The scores add to the growing body of evidence placing Lagerpeptidae into Pterosauromorpha but fall short of any clear evidence regarding Silesauridae.
How does fine root influence aggregates? comparing root diameter distribution of two herbaceous plants: a pot experiment
Authors: Kang Zijian , Lou Guochong , Guo Yuanyuan , Xu Peng 
Published: June 2023

Abstract
Purpose
The main objective of this study was to investigate the impact and importance of herb fine roots in different diameter ranges on soil aggregate stability.
Materials and methods
A laboratory experiment was conducted to disentangle and evaluate the effect of fine roots on soil aggregates. Two herbs, alfalfa (Medicago sativa) with taproots and tall festuca (Festuca arundinacea) with fibrous roots, were selected for single and mixed planting. Root configuration and aggregate stability were measured after different growth periods (30, 60, and 120D).
Results and discussion
During the growth period of our study, fine roots (< 0.5mm) accounted for the vast majority in soil, while coarse roots (> 1mm) appeared only in soil containing taproots. Planting could improve the mechanical and water stability of aggregates, with enhancement effects displayed as tall festuca > alfalfa, and mixed planting > single planting. Mechanical stability has a higher demand for finer roots. The density increase of very fine roots (< 0.25mm) can improve mechanical stability continuously, but other diameter roots, especially for diameter > 1mm, will have adverse effects on stability after the density reaches a certain threshold. Roots in each diameter range can improve aggregate water stability, but the correlation between root density and water-stable indices decreased gradually with the increase of root diameter.
Conclusions
The higher ratio of finer roots makes fibrous roots have a better effect on aggregate stability than taproots. Fine roots improve the stability of aggregates mainly by combining 0.5-5mm aggregates to form > 5mm aggregates, and soil organic matter provides raw materials for this process by combining microaggregates (< 0.25mm). RLD is a suitable parameter for predicting changes in aggregate stability, and finer roots could explain more variation in aggregate stability.
Functional connectivity of the amygdala subnuclei in various mood states of bipolar disorder
Authors: KRYSTAL Sidney , Gracia Laure , Piguet Camille , Henry Chantal , Alonso Mariana , Polosan Mircea , Savatovsky Julien , Houenou Josselin , Favre Pauline 
Published: June 2023

Abstract

Amygdala functional dysconnectivity lies at the heart of the pathophysiology of bipolar disorder (BD). Recent preclinical studies suggest that the amygdala is a heterogeneous group of nuclei, whose specific connectivity could drive positive or negative emotional valence. We investigated functional connectivity (FC) changes within these circuits emerging from each amygdala’s subdivision in 127 patients with BD in different mood states and 131 healthy controls (HC), who underwent resting-state functional MRI. FC was evaluated between lateral and medial nuclei of amygdalae (lAMY-rh, mAMY-rh, lAMY-lh, mAMY-lh), and key subcortical regions of the emotion processing network: anterior and posterior parts of the hippocampus (aHIP, pHIP), and core and shell parts of the nucleus accumbens (NAc-core and NAc-shell). FC was compared across groups, and subgroups of patients depending on their mood states, using linear mixed models. We also tested correlations between FC and depression (MADRS) and mania (YMRS) scores. We found no difference between the whole sample of BD patients vs. HC but a significant correlation between MADRS and lAMY-rh/aHIP-rh, lAMY-rh/pHIP-rh and lAMY-rh/aHIP-lh FC (r=-0.53, r=-0.36, r=-0.28, respectively, all pFDR<0.05). Subgroup analysis revealed decreased lAMY-rh/aHIP-rh and lAMY-rh/pHIP-rh FC in depressed vs. non-depressed patients, and increased mAMY-lh/NAc-shell-lh FC in manic vs non-manic patients. These results demonstrate that acute mood states in BD concur with FC changes in individual nuclei of the amygdala implicated in distinct emotional valence processing. Overall, our data highlight the importance to consider the amygdala subnuclei separately when studying its FC patterns including patients in distinct homogeneous mood states.
Not taking sick leave is negatively associated with women undergoing gynecologic cancer treatment returning to the same workplace
Authors: nakamura keiichiro , Matsuoka Hirofumi , Kubo Kotaro , Shirakawa Shinsuke , Ida Naoyuki , Haraga Junko , Ogawa Chikako , Okamoto Kazuhiro , Nagao Shoji , Masuyama Hisashi 
Published: June 2023

Abstract
BACKGROUND: Gynecologic cancers are one of the most common types of malignancies in working-age women. We aimed to determine which factors impede women from returning to the same workplace after treatment for such cancers.
METHODS: A questionnaire-based survey was conducted on 194 women who underwent treatment for gynecologic cancer at Okayama University (≥1 year after cancer treatment and <65 years of age). We compared patient characteristics by using the Mann–Whitney U-test. We also performed univariate logistic regression analysis to determine the relationship between returning to the same workplace and several patient characteristics.
RESULTS: The median age at diagnosis was 49.0 years, and the median time from cancer treatment to questionnaire completion was 3.8 years. Not returning to the same workplace was positively associated with not taking sick leave (P<0.001). Not taking sick leave was positively associated with working ≤5 hours per day and having a low personal income (P=0.049 and P=0.018, respectively). Time to return to the same workplace was positively related to the duration of cancer treatment (P=0.002).
CONCLUSION: Not taking sick leave likely was negatively associated with returning to the same workplace after treatment for gynecologic cancer. Therefore, we suggest that steps be taken to formally introduce a sick leave system over and above the paid leave system in Japan.
The effect of 12-weeks of head down and strong abdominal breathing on cognitive function in patients with stable COPD under dual task: a singer randomized controlled trial study protocol
Authors: Ding Kexin , Song Feiyun , Sun Wen , Liu Hongrui , Sun Mingyun 
Published: June 2023

Abstract
Background: Chronic obstructive pulmonary disease (COPD) is an irreversible disease characterised by persistent respiratory symptoms and decreased airflow. To improve the overall clinical picture of COPD, it is necessary to better understand all relevant comorbidities. Despite the fact that impairments in cognitive function are linked to higher mortality and disability rates in COPD, little is known about how these impairments can be improved. The purpose of this study is to investigate how head down and strong abdominal breathing and three other interventions affect oxygen utilization in COPD patients' dorsolateral prefrontal cortex (PFC) and general and specific cognitive domains, as well as to fill gaps in clinical evidence related to these interventions.
Methods: We recruit 108 COPD patients in stable GOLD I-II stages at the Naval Anqing Hospital, using the blind method of evaluators and data analysts. There are four groups: head down and strong abdominal breathing training (Angle of inclination to 60°) , Ba Duan Jin, strong abdominal breathing training, and conventional treatment. Respiratory frequency is 35 breaths/min for 40 minutes three times a week for 12 weeks. The primary outcome is an assessment of general and four specific cognitive domains of function, as well as functional near infrared spectroscopy (fNIRS) recorded in the dorsolateral PFC of patients with normal or fast walking while performing motor or cognitive dual tasks containing oxygenated haemoglobin (oxy-Hb), deoxygenated haemoglobin (deoxy-Hb), and total oxygenated haemoglobin (total-Hb). Secondary outcomes include dyspnea symptoms, executive functions, anxiety and depressive symptoms, and balance. The outcomes will be measured one week before, six weeks after, and 12 weeks after.
Discussion: The study may determine how effective head down and strong abdominal breathing exercises are for COPD patients with mild to moderate stable COPD. Also, it may help determine if it's more effective than other pulmonary rehabilitation treatments.
Differential diagnosis of unipolar versus bipolar depression by GSK3 levels in peripheral blood: a pilot experimental study.
Authors: Rosso Gianluca , Maina Giuseppe , Teobaldi Elena , Balbo Ilaria , Salvo Gabriele Di , Montarolo Francesca , Pesci Nicola Rizzo , Tempia Filippo , Hoxha Eriola 
Published: June 2023

Abstract
Background
The differential diagnosis of patients presenting for the first time with a depressive episode into unipolar disorder versus bipolar disorder is crucial to establish the correct pharmacological therapy (antidepressants vs mood stabilizers), but no biological markers are currently available. Several lines of evidence indicate an involvement of Glycogen Synthase Kinase-3 (GSK3) in the pathophysiology of depression. However, previous reports about GSK3 in peripheral blood were incomplete or inconsistent, so a specific marker is not yet available. The aim was to search for consistent differences in GSK3α and GSK3β or of their phosphorylated forms in samples of peripheral blood from patients with unipolar and bipolar depression.
Methods
Mononucleate peripheral blood cells (PBMCs) of samples from patients presenting with a depressive episode were analyzed with the western blot technique.
Results
The total amount of GSK3β in PBMCs was significantly lower in patients with bipolar disorder than in patients with unipolar depression. The sensitivity based on GSK3β was 85%. GSK3α was not significantly different but allowed a correct detection of 57% of BD patients. The combination in series of GSK3β and GSK3α yields a sensitivity of about 100%, but with 26.7% false negatives.
Conclusions
Our results suggest that PBMC GSK3β could be a candidate biomarker for the differential diagnosis of bipolar disorder versus unipolar depression. To our knowledge, this is the first identification of an easily detectable biological marker that enables a differential diagnosis between unipolar and bipolar disorder in patients presenting for with a depressive episode.
Integrating brain function and structure in the study of the human attentional networks
Authors: Signes Mar Martín , Paz-Alonso Pedro , de Schotten Michel Thiebaut , Chica Ana 
Published: June 2023

Abstract
Attention is a heterogeneous function theoretically divided into alerting, orienting, and executive attention. While functional magnetic resonance imaging (fMRI) has extensively characterized their functioning, the role of white matter in cognitive function has gained recent interest due to diffusion-weighted imaging advancements. However, most evidence relies on correlations between white matter properties and behavioral or cognitive measures. This study used a new method that combines the signal from distant voxels of fMRI images using the probability of structural connection given by a high-resolution normative tractography. We analyzed three fMRI datasets with a visual perceptual task and three attentional manipulations (phasic alerting, spatial orienting, and executive attention). The phasic alerting network engaged temporal areas and their communication with frontal and parietal regions, with a left hemisphere dominance. The orienting network involved bilateral fronto-parietal and midline regions communicating by association tracts and interhemispheric fibers. The executive attention network engaged a broad set of brain regions and white matter tracts connecting them, with a particular involvement of frontal areas and their connections with the rest of the brain. These results confirm and extend previous knowledge on the neural substrates of the attentional system, offering a more comprehensive understanding by integrating structure, function, and behavior.
IFR estimation of overlapped multicomponent signals from time frequency rate distribution
Authors: Li Po , Dong Peng , Luo Qiao 
Published: June 2023

Abstract
Instantaneous frequency rate (IFR) estimation from multi-component signals can be applied in a variety of applications. Viterbi algorithm (VA) on cubic phase function (CPF) is a highly performed IFR estimator for the mono-component signal. However, for the multi-component signal, when signal components are overlapped on the time frequency rate (TFR) plane, inaccurate IFRs may be tracked due to switch problem (SP) in VA and cross-terms in CPF. To overwhelm these two challenges, a directionally smoothed pseudo CPF (DSPCPF) is firstly proposed, the algorithm assumes that the IFR curve can be approximately linear along a direction within an adequately short time window, at a time instant, while the TFR points corresponding to cross-terms are dispersed in a entire TFR plane, then, TFR points of CPF along the IFR curve are integrated to enhance the auto-terms and suppress the cross-terms. In addition, to suppress the SP, the direction matrix of DSPCPF is utilized to construct an additional directional penalty function for VA. The new VA combined with DSPCPF is applied to estimate IFRs. In this way, more accurate IFRs can be obtained, and the performance of novel algorithms are validated by several artificial multi-component frequency modulated signals.
Urinary incontinence and its associated factors in nursing home residents: a multi-centre cross-sectional study in Central Catalonia
Authors: Jerez-Roig Javier , Farrés-Godayol Pau , Yildirim Meltem , Escribà-Salvans Anna , Moreno-Martin Pau , Goutan-Roura Ester , Rierola-Fochs Sandra , Romero-Mas Montse , Booth Joanne , Skelton Dawn , Giné-Garriga Maria , Minobes-Molina Eduard 
Published: June 2023

Abstract
Background
Urinary incontinence (UI) is a common geriatric syndrome with high health and socio-economic impacts in nursing home (NH) residents.
Objectives
To verify the prevalence and types of UI and its associated factors in older people living in NHs in Central Catalonia (Spain). We also determined the proportion of residents who were receiving behavioural strategies to prevent/manage UI.
Design and setting
Cross-sectional study in 5 NHs conducted from January to March 2020.
Methods
We included consenting residents aged 65 + permanently living in the NHs. Residents who were hospitalized, in a coma or palliative care were excluded. UI was assessed using Section H of the Minimum Data Set. Sociodemographic and health-related variables were examined. Descriptive, bivariate and multivariate (logistic regression) analyses were performed.
Results
We included 132 subjects (82.6% women), mean age of 85.2 (SD = 7.4) years. The prevalence of UI was 76.5% (95% CI: 68.60-82.93). The most common type was functional UI (45.5%), followed by urgency UI (11.4%). Most sedentary behaviour variables were significant in the bivariate analyses, but none remained in the final model. Moderate-severe cognitive impairment (OR = 4.44, p = .003), anticholinergic activity (OR = 3.50, p = .004) and risk of sarcopenia using SARC-F (OR = 2.75, p = .041) were significantly associated with UI. Only 46.2% of residents received any behavioural strategy to manage UI.
Conclusions
The prevalence of UI was high in this sample of NH residents compared to the literature, yet less than half received prompted voiding as a strategy to prevent/reduce UI. UI was associated with cognitive impairment, anticholinergic activity, and risk of sarcopenia.
Thermoring basis for the TRPV3 bio-thermometer
Authors: Wang Guangyu 
Published: June 2023

Abstract
The thermosensitive transient receptor potential (TRP) channels are well-known as bio-thermometers with specific temperature thresholds and sensitivity. However, their structural origins are still mysterious. Here, graph theory was used to test how the temperature-dependent non-covalent interactions as identified in the 3D structures of thermo-gated TRPV3 could form a systematic fluidic grid-like mesh network with the thermal rings from the biggest grids to the smallest ones as necessary structural motifs for the variable temperature thresholds and sensitivity. The results showed that the heat-evoked melting of the biggest grids may control temperature thresholds to activate the channel while the smaller grids may act as thermo-stable anchors to secure the channel activity. Together, all the grids along the gating pathway may be necessary for the specific temperature sensitivity. Therefore, this grid thermodynamic model may provide an extensive structural basis for the thermo-gated TRP channels.
FishDNAIDs: DNA barcoding as a tool in the development of a detection system to four species of Characiformes of commercial importance in the Brazilian Amazon
Authors: Bevilaqua Danniel Rocha , Batista Jacqueline da SIlva , Mota Adolfo José da , Silva Ana Caroline Viana da , Mota Andreza Mikeyne Silva da , Formiga Kyara Martins , Freitas Carlos Edwar de Carvalho 
Published: June 2023

Abstract
Backgroud The COI mitochondrial gene has been chosen as the “DNA barcode in animals” and the large quantity of genetic information in public databanks gives solid support for the use of DNA barcoding as a promising tool for the development of a specific molecular detection system.
Methods and results The present study aimed to develop a specific molecular detection system (SMDS) (primers and probe sets) for the following four target species: Prochilodus nigricans, Potamorhina altamazonica, Psectrogaster rutiloides and Triportheus angulatus, in qPCR assays. In silico and in vitro tests (using gDNA) were performed to test these sets. The database generated contained the COI nucleotide sequence for 187 specimens of Characiformes, distributed in 35 species representing eight families. In silico, primers designed for the target species amplified different species from the same genus, except for P. rutiloides, which amplified only the target species. In the in vitro test, using the SYBRGreentm and TaqMan® fluorescence systems, both sets detected the respective target species (P. nigricans, P. altamazonica, P. rutiloides and T. angulatus). In the qPCR assays using SYBRGreentm, species considered to be related were also detected, in addition to the target species, with the exception of P. amazonica and P. essequibensis (correlated to P. rutiloides). All target species were detected in the qPCR assays using the TaqMan® system; however, with the SMDS PALT, the target species P. altamazonica was detected with low CT values (22.21± 0.17) as well as the correlates of P. latior and P. pristigaster, though with high CT values (23.51 ± 0.19 and 30.21 ± 0.95). This assay uniquely identifies known adult tissue samples from all four species.
Conclusions The primers and probe sets developed can act as powerful tools for detecting the target Characiformes species.
GCMR: General Cross-Modality Registration Framework for Visible and Infrared UAV Target Image Registration
Authors: Luo Yu , Cha Hao , Zuo Lei , Cheng Peng , Zhao Qing 
Published: June 2023

Abstract
In all-day-all-weather tasks, well-aligned multi-modality images pairs can provide extensive complementary information for image-guided UAV target detection. However, multi-modality images in real scenarios are often misaligned, and images registration is extremely difficult due to spatial deformation and the difficulty narrowing cross-modality discrepancy. To better overcome the obstacle, in this paper, we construct a General Cross-Modality Registration (GCMR) Framework, which explores generation registration pattern to simplify the cross-modality image alignment into a easier mono-modality registration with an Image Cross-modality translation Network (ICMTN) module and a Multi-level Residual Dense Registration Network (MRDRN). Specifically, ICMTN module is used to generate a pseudo infrared image taking a visible image as input and correct the distortion of structural information during the translation of image modalities. Benefiting from the favorable geometry correct ability of the ICMTN, we further employs MRDRN module which can fully extract and exploit the mutual information of misaligned images to better registered Visible and Infrared image in a mono-modality setting. We evaluate five variants of our approach on the public Anti-UAV datasets. The extensive experimental results demonstrate that the proposed architecture achieves state-of-the-art performance.