bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design and fabrication of a microfluidic system with embedded circular channels for rotary cell culture
Authors: Mitxelena‐Iribarren Oihane , Bujanda Xabier , Zabalza Laura , Alkorta Janire , Lopez‐Elorza Aitziber , Gracia Raquel , Dupin Damien , Arana Sergio , Ruiz‐Cabello Jesús , Mujika Maite 
Published: April 2023

AbstractThe development of functional blood vessels is today a fundamental pillar in the evaluation of new therapies and diagnostic agents. This article describes the manufacture and subsequent functionalization, by means of cell culture, of a microfluidic device with a circular section. Its purpose is to simulate a blood vessel in order to test new treatments for pulmonary arterial hypertension. The manufacture was carried out using a process in which a wire with a circular section determines the dimensions of the channel. To fabricate the blood vessel, cells were seeded under rotary cell culture to obtain a homogeneous cell seeding in the inner wall of the devices. This is a simple and reproducible method that allows the generation of blood vessel models in vitro.
Humoral and cellular immune response to second and third severe acute respiratory syndrome coronavirus 2 mRNA vaccine in patients with plasma cell dyscrasia
Authors: Suzuki Tomotaka , Kusumoto Shigeru , Kamezaki Yoshiko , Hashimoto Hiroya , Nishitarumizu Nozomi , Nakanishi Yoko , Kato Yukiyasu , Kawai Akimi , Matsunaga Naohiro , Ebina Toru , Nakamura Tomoyuki , Marumo Yoshiaki , Oiwa Kana , Kinoshita Shiori , Narita Tomoko , Ito Asahi , Inagaki Atsushi , Ri Masaki , Komatsu Hirokazu , Aritsu Takashi , Iida Shinsuke 
Published: April 2023

AbstractBackgroundThe recently developed severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) mRNA vaccine has a short history of use and further information is needed regarding its efficacy, especially in immunocompromised conditions, such as plasma cell dyscrasia (PCD).MethodsWe retrospectively measured serum SARS‐CoV‐2 antibodies against the spike protein (S‐IgG) after the second and third mRNA vaccine doses (doses 2 and 3, respectively) in 109 patients with PCD. We evaluated the proportion of patients with an adequate humoral response (defined as S‐IgG titers ≥300 antibody units/mL).ResultsAlthough active anti‐myeloma treatments prior to vaccination had a significantly negative impact on adequate humoral response, specific drug subclasses including immunomodulatory drugs, proteasome inhibitors, and monoclonal antibodies were not negatively associated, except for B‐cell maturation antigen‐targeted therapy. Dose 3 (booster vaccination) led to significantly higher S‐IgG titers and more patients acquired an adequate humoral response. Furthermore, evaluation of vaccine‐induced cellular immune response in patients using T‐spot Discovery SARS‐CoV‐2 kit, revealed an enhanced cellular immune response after Dose 3.ConclusionsThis study highlighted the significance of booster SARS‐CoV‐2 mRNA vaccination in patients with PCD with respect to humoral and cellular immunity. Moreover, this study highlighted the potential impact of certain drug subclasses on vaccine‐induced humoral immune response.
Relationship between coronary volume, myocardial mass, and post‐PCI fractional flow reserve
Authors: Mileva Niya , Ohashi Hirofumi , Paolisso Pasquale , Leipsic Jonathon , Mizukami Takuya , Sonck Jeroen , Norgaard Bjarne L. , Otake Hiromasa , Ko Brian , Maeng Michael , Munhoz Daniel , Nagumo Sakura , Belmonte Marta , Vassilev Dobrin , Andreini Daniele , Barbato Emanuele , Koo Bon‐Kwon , De Bruyne Bernard , Collet Carlos 
Published: April 2023

AbstractBackgroundFractional flow reserve (FFR) measured after percutaneous coronary intervention (PCI) carries prognostic information. Yet, myocardial mass subtended by a stenosis influences FFR. We hypothesized that a smaller coronary lumen volume and a large myocardial mass might be associated with lower post‐PCI FFR.AimWe sought to assess the relationship between vessel volume, myocardial mass, and post‐PCI FFR.MethodsThis was a subanalysis with an international prospective study of patients with significant lesions (FFR ≤ 0.80) undergoing PCI. Territory‐specific myocardial mass was calculated from coronary computed tomography angiography (CCTA) using the Voronoi's algorithm. Vessel volume was extracted from quantitative CCTA analysis. Resting full‐cycle ratio (RFR) and FFR were measured before and after PCI. We assessed the association between coronary lumen volume (V) and its related myocardial mass (M), and the percent of total myocardial mass (%M) with post‐PCI FFR.ResultsWe studied 120 patients (123 vessels: 94 left anterior descending arteries, 13 left Circumflex arteries, 16 right coronary arteries). Mean vessel‐specific mass was 61 ± 23.1 g (%M 39.6 ± 11.7%). The mean post‐PCI FFR was 0.88 ± 0.06 FFR units. Post‐PCI FFR values were lower in vessels subtending higher mass (0.87 ± 0.05 vs. 0.89 ± 0.07, p = 0.047), and with lower V/M ratio (0.87 ± 0.06 vs. 0.89 ± 0.07, p = 0.02). V/M ratio correlated significantly with post‐PCI RFR and FFR (RFR r = 0.37, 95% CI: 0.21−0.52, p < 0.001 and FFR r = 0.41, 95% CI: 0.26−0.55, p < 0.001).ConclusionPost‐PCI RFR and FFR are associated with the subtended myocardial mass and the coronary volume to mass ratio. Vessels with higher mass and lower V/M ratio have lower post‐PCI RFR and FFR.
Lead‐Free Perovskite Cs2SnBr6/rGO Composite for Photocatalytic Selective Oxidation of 5‐Hydroxymethylfurfural to 2,5‐Diformylfuran
Authors: Yin Liwen , Wu Yaqiang , Bao Xiaolei , Liu Xiaolei , Dai Dujuan , Zhang Minghui , Wang Zeyan , Zheng Zhaoke , Liu Yuanyuan , Cheng Hefeng , Dai Ying , Huang Baibiao , Wang Peng 
Published: April 2023

AbstractSevere poisonousness and prolonged instability existing in organic‐inorganic lead‐based perovskite are two matters seriously hindering its potential future application in photocatalysis. Therefore, it is particularly important to explore ecology‐friendly, air‐stable and highly active metal‐halide perovskites. Herein, a new and stable lead‐free perovskite Cs2SnBr6 decorated with reduced graphene oxide (rGO), is synthesized and employed in the photocatalytic organic conversion. The as‐prepared Cs2SnBr6 is ultrastable, exhibiting no clear changes after being placed in the air for six months. The Cs2SnBr6/rGO composite shows excellent photocatalytic activity in photo‐driven‐oxidation of 5‐hydroxymethylfurfural (HMF) to high value enclosed 2,5‐diformylfuran (DFF), achieving>99.5 % conversion of HMF and 88 % DFF selectivity in the presence of green oxidant O2. Comprehensive characterizations disclose a multistep reaction mechanism, demonstrating that the molecular oxygen, photogenerated carriers, ⋅O2− and 1O2 altogether synergistically participate in the effective photo‐driven conversion of HMF to DFF. This work expands the material gallery towards selective organic conversion and environmentally friendly perovskite options for photocatalytic application.
σ,π‐Conjugated Bis(germylene) Adducts with NHC and CAACs
Authors: Thömmes Anna‐Lena , Morgenstern Bernd , Zimmer Michael , Andrada Diego M. , Scheschkewitz David 
Published: April 2023

AbstractHeavier tetrylenes attract attention for their potential in synthesis, catalysis and small molecule activation. The coordination byN‐heterocyclic carbenes (NHCs) and cyclic (alkyl)(amino)carbenes (CAACs) results in substantial structural and electronic differences although typically only one of these yields stable derivatives for one and the same tetrylene. We now report both NHC‐ and CAAC‐coordination to a bridged bis(germylene) motif. The NHC‐coordinated bis(germylene) exhibits pyramidal germanium centers with lone pairs of electrons, while with CAAC an unprecedented stable bis(germene) with two Ge=C bonds is isolated. Spectroscopic and crystallographic evidence as well as DFT calculations confirm the effects of σ,π‐conjugation between the two germanium centers in both cases. The coordination of NHC is reversible as the reaction with BPh3liberates the transient bis(germylene) and thus provides an alternative low‐temperature route towards polymers with Ge=Ge bonds.
Proton acceleration from TiH2 target plasma produced by different pulse laser intensity
Authors: Torrisi L. , Torrisi A. 
Published: April 2023

AbstractThe laser irradiation of TiH2 targets has been investigated to measure the proton energy transferred by hot non‐equilibrium plasmas in vacuum. The proton and titanium emission have been monitored by time‐of‐flight measurements to evaluate their maximum ion energy and dependence on the laser intensity. Backward and forward plasma have been generated in the range 1010–1016 W/cm2 intensity using ns and sub‐ns laser shots. Proton energy has been measured by tens eV up to about 4 MeV. The interest in proton beams generated by laser‐plasma is presented and discussed.
Bethesda system reporting rates, histological follow‐up and HPV genotypes distribution of abnormal cytology in Anhui province of China: Analysis of 93,168 cervical liquid‐based cytology test reports
Authors: Xu Hongliang , Chen Yong , Zhao Caixia , Wang Qin , Tang Man , Zhang Weiqin , Wang Qing , Lou Rong , Liu Tingting , Feng Yi , Zhang Heping 
Published: April 2023

AbstractObjectiveTo analyze the Bethesda System reporting rates, histological follow‐up, and HPV genotypes distribution of abnormal cytology in Anhui province of China.MethodsAccording to the Bethesda Reporting System of Cervical Cytology (2014), a retrospective analysis of the cervical liquid‐based cytology (LBC) results, abnormal cytology with concurrent HPV genotype testing, and immediate histological follow‐up. HPV genotype testing was performed for 15 High‐risk types and 6 Low‐risk types. Immediate histological correlation results within 6 months after the LBC and HPV results.Results6.70% of women with abnormal LBC results, and ASC/SIL was 1.42. The severe histological results in abnormal cytology were ASC‐US (18.58%), ASC‐H (53.76%), LSIL (16.62%), HSIL (82.07%), SCC/ACa (100.00%), AGC (63.77%). The total HPV‐positive rate in abnormal cytology was 70.29%, of which ASC‐US, ASC‐H, LSIL, HSIL, SCC/ACa, and AGC were 60.78%, 80.83%, 83.05%, 84.93%, 84.51%, 33.33%. The top three detected genotypes were HR HPV 16, 52, and 58. The most commonly detected genotype in HSIL and SCC/ACa was HPV 16. Of the 91 AGC patients, 34.78% were cervical lesions, and 42.03% were endometrial lesions. The HPV‐positive rate in the group of AGC‐FN was highest and lowest in the group of AGC‐EM.ConclusionThe Bethesda System reporting rates of cervical cytology were all within the benchmark range of the CAP laboratory. HPV 16, 52, and 58 were the most common genotypes in our population, and HPV 16 infection has a higher degree of malignancy of cervical lesions. Among patients with ASC‐US results, HPV positive patients had a higher rate of biopsy‐detected CIN2+ than HPV negative patients.
All‐trans retinoic acid inhibits oxidative stress via ACE2/Ang (1–7)/MasR pathway in renal tubular epithelial cells stimulated with high glucose
Authors: Zhu Xiaojuan , Mou Zhixiang , Han Wei , Chen Lan 
Published: April 2023

AbstractThe aim of this study was to investigate the effects of all‐trans retinoic acid (atRA) on oxidative stress in renal tubular epithelial cells induced by high glucose (HG) and its potential mechanism. We investigated the effects of atRA in HG‐induced renal epithelial cell line HK‐2. Seven groups were designed for this experiment: negative control, mannitol, high‐glucose (HG), HG combined with a low concentration of atRA, HG combined with a middle concentration of atRA, HG combined with a high concentration of atRA, and HG combined with captopril. After 48 h of incubation, oxidative stress factor expression in the supernatant was detected by enzyme‐linked immunosorbent assay. Reactive oxygen species and cell apoptosis expression were assessed by flow cytometry. NADPH oxidase, fibrosis factor, and angiotensin‐converting enzyme 2/angiotensin (1–7)/mas receptor (ACE2/Ang (1–7)/MasR) pathway‐related protein expressions were determined by western blot analysis. The expressions of oxidative stress factors, NADPH oxidase components, and fibrosis factors were significantly higher after HG treatment. Apoptosis of HK2 cells in the HG group was also significantly higher. AtRA could reverse the above abnormal changes in a concentration‐dependent manner. HG significantly promoted the expression of ACE, Ang II, and Ang II type 1 receptor (AT1R), whereas it inhibited the expression of ACE2, Ang (1–7), and MasR. With the elevation of concentration, atRA could gradually suppress the expression of ACE, Ang II, and AT1R, but facilitate ACE2, Ang (1–7), and MasR. These results were statistically significant. AtRA could significantly inhibit oxidative stress and apoptosis of renal tubular epithelial cells induced by HG. The mechanism may inhibit the ACE/Ang II/AT1R pathway and/or activate ACE2/Ang (1–7)/MasR pathway.
Future of dermal fillers
Authors: Strat Nicholas , Snyder Alan , Schlesinger Todd 
Published: April 2023

AbstractThe advancement of dermal fillers has progressed from animal collagen extraction to a spectrum of recombinant DNA, nanotechnology, and biostimulation. Consumers now have a variety of fillers to choose from, including organic fillers composed of hyaluronic acid and calcium hydroxyapatite, as well as synthetic fillers made from poly‐l‐lactic acid and polymethylmethacrylate. This piece serves to add to the open dialogue discussing the prognosis, prospects, and potential pitfalls of dermal fillers. Expansion of product indications and technical alterations are currently changing the dermal fillers' landscape. The potential for new approaches to side effect mitigation or adjunct pigment modification is still exploratory. Furthermore, with social implications increasingly coming into frame, healthcare providers and companies seem geared toward developing effective, safe, and equitable products while maintaining a process of rigorous review and quality control processes. As such, future developments of this medical tool appear promising; fillers seem poised to imbue a role as dynamic as the tissues they were conceived to restore.
Phylogeography and cohesion species delimitation of California endemic trapdoor spiders within the Aptostichus icenoglei sibling species complex (Araneae: Mygalomorphae: Euctenizidae)
Authors: Newton Lacie G. , Starrett James , Jochim Emma E. , Bond Jason E. 
Published: April 2023

AbstractSpecies delimitation is an imperative first step toward understanding Earth's biodiversity, yet what constitutes a species and the relative importance of the various processes by which new species arise continue to be debatable. Species delimitation in spiders has traditionally used morphological characters; however, certain mygalomorph spiders exhibit morphological homogeneity despite long periods of population‐level isolation, absence of gene flow, and consequent high degrees of molecular divergence. Studies have shown strong geographic structuring and significant genetic divergence among several species complexes within the trapdoor spider genus Aptostichus, most of which are restricted to the California Floristic Province (CAFP) biodiversity hotspot. Specifically, the Aptostichus icenoglei complex, which comprises the three sibling species, A. barackobamai, A. isabella, and A. icenoglei, exhibits evidence of cryptic mitochondrial DNA diversity throughout their ranges in Northern, Central, and Southern California. Our study aimed to explicitly test species hypotheses within this assemblage by implementing a cohesion species‐based approach. We used genomic‐scale data (ultraconserved elements, UCEs) to first evaluate genetic exchangeability and then assessed ecological interchangeability of genetic lineages. Biogeographical analysis was used to assess the likelihood of dispersal versus vicariance events that may have influenced speciation pattern and process across the CAFP's complex geologic and topographic landscape. Considering the lack of congruence across data types and analyses, we take a more conservative approach by retaining species boundaries within A. icenoglei.
Synthesis of Methylene Tetrahydrofurane‐Fused Carbohydrates
Authors: Ahmadian‐Moghaddam Maryam , Reintjens Niels R. M. , Witte Martin D. , Minnaard Adriaan J. 
Published: April 2023

AbstractA reliable method is disclosed to introduce a fused methylene tetrahydrofuran ring into carbohydrates. The resulting bicyclic saccharides can be used as scaffolds in medicinal chemistry and drug design. In addition, the enol ether functionality serves as a handle that enables modification in biological systems via photoclick chemistry. The approach is based on the regioselective oxidation of the C‐3 hydroxy group in gluco‐configured pyranosides, followed by stereoselective indium‐mediated allylation. The ring formation is induced by an iodocyclization reaction with a neighboring hydroxy group. Subsequent dehydrohalogenation affords the desired methylene‐tetrahydrofuran‐containing carbohydrates.
Materials and device designs for thermophotovoltaic power conversion
Authors: McCann Patrick J. , Khodr Majed 
Published: April 2023

AbstractTwo IV–VI semiconductor alloys, Pb0.81Sn0.19Se and Pb0.8Sr0.2Se, are proposed for use in designing multiple quantum well (MQW) materials and devices for thermophotovoltaic (TPV) power conversion. These materials can be epitaxially grown on silicon substrates, so they offer the potential for a low cost TPV device manufacturing technology. MQW materials examples are provided for fabricating triple junction TPV devices for power conversion with a 1400°C radiator. Optimal n‐type and p‐type layer thicknesses for each junction were determined using internal quantum efficiency expressions derived assuming all photogenerated charge collection is by diffusion. Depending on MQW material quality and assumed optical absorption levels, predicted power generation for current matched triple junction devices ranged from 1.7 to 4.6 W/cm2 and power conversion efficiencies ranged from 15.9% to 35.7%. These device performance parameters were used in a levelized cost of energy (LCOE) calculation to predict the cost of energy provided by TPV devices with a molten silicon thermal energy storage system. Results show that even for TPV devices fabricated from low quality materials, such energy storage systems will have a low enough LCOE to be competitive with other forms of energy storage and power generation.
Effect of plasma jet on electrochemical properties of silk fibroin hydrogel doped with PEDOT:PSS
Authors: Zhou Jian , Lu Fu , Cong Jing , Wu Zhengwei 
Published: April 2023

AbstractIn this study, by injecting cold plasma into the silk fibroin (SF) solution, and adding poly (3,4‐ethylenedioxythiophene): polystyrene sulfonic acid (PEDOT:PSS) into the SF solution, the conductive SF‐PEDOT:PSS hydrogel modified by plasma was successfully prepared. The changes of the electrochemical parameters and structure of SF‐PEDOT:PSS hydrogels after plasma modification were observed with plasma discharge time (60, 120, 180 s) and the type of working gas (Ar, air, He) as variables. The results of electrochemical impedance spectroscopy show that both argon plasma and helium plasma can reduce the impedance of SF‐PEDOT:PSS hydrogel in the extremely low‐frequency range (10−2–10° Hz), and a longer discharge time shrinks the impedance, while the impact of air plasma on the impedance of hydrogel is not obvious. The capacitance results show that both argon and helium plasma can make the capacitance of hydrogel larger, and the longer discharge time leads to the larger capacitance. While air plasma can reduce the capacitance of hydrogel. The results of Fourier infrared absorption spectroscopy and X‐ray diffraction spectrum show that the secondary structure of SF is mainly β‐fold. The results of Raman show that PEDOT:PSS is successfully incorporated into hydrogel, and there exists random coil conformation of SF in hydrogel. The results of X‐ray photoelectron spectroscopy show that the SF‐PEDOT:PSS hydrogel mainly contains C, O, and N elements.
Investigation of the process of perforating hard roof in an innovative technology of directional roof‐cutting by composite blasting
Authors: Ma Bochao , Tai Yang , Li Yong , Xia Hongchun , Meng Xiangbin 
Published: April 2023

AbstractThe hard roof in coal mines will induce strong pressure problems for the working face and the lanes, which will seriously affect the safe and efficient production of the mine. For these issues, our team developed a new technology of cutting hard roof by composite blasting. With the new technology, a penetrating process is key to the directionally cut roof. Thus, an arbitrary Lagrange–Euler method is proposed to calculate the penetrating process. In this method, it was taken that the Jones–Wilkins–Lee constitutive model, Johnson–Cook constitutive model, and Holmquist–Johnson–Cook constitutive model to build a numerical model, by LS‐DYNA software, of the process of the penetrating the hard roof. Finally, the following conclusions were obtained: (1) The velocity variation law of metal liner from head to tail is revealed. The speeds of the metal liner's head and tail are 3500 and 450 m/s, respectively. (2) The shape of the channel formed by shaped charge jets is like a funnel, whose mouth's depth and diameter are 33 and 49 mm, respectively. The funnel's narrow diameter is 7.5 mm. (3) With the increase of the channel depth, the rock damage area changes in three different states: slow increase, rapid increase, and linear increase. Then, a shovel‐shaped geometric model of rock damage zone is established, whose key size parameters in the model are given.