bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of critical hypoperfusion biomarkers on CT with futile recanalization and poor outcome after mechanical thrombectomy in acute ischemic stroke
Authors: Fu Meng , Yang Jun , Dong Xiaonan , Huang Changren , Yuan Zhengzhou , Jiang Li , Meng Renliang , Xie Yang , Li Jinglun 
Published: June 2023

Abstract

Background and Purpose
We aimed to investigate the association between critical perfusion delay and poor outcome among recanalized stroke patients with anterior large-vessel occlusion, and to use pretreatment hypoperfusion biomarkers on CT to predict futile recanalization even after successful thrombectomy.
Methods
An ischemic region with time-to-maximum (Tmax) > 12s or 10s was defined as critical hypoperfusion, Tmax > 8s as moderate hypoperfusion, and hypoperfusion intensity ratio (HIR, volumetric ratio of Tmax > 10s / Tmax > 6s) represented for severity of critical hypoperfusion and rCBF < 30% for ischemic core. The associations between these CT perfusion characteristics and favorable or unfavorable outcome (mRS 0–2 versus 3–6) were analyzed in univariable regression, and a multivariable model was then used to predict futile recanalization.
Results
Seventy-nine stroke patients were included and had good grades of instant recanalization. Forty-two patients (53%) had poor outcomes, and they had a significantly larger volume of critical hypoperfusion as seen with Tmax > 10s and > 12s (P = 0.032 and 0.008, respectively), a larger volume of ischemic core (P = 0.011) and a higher HIR (P = 0.002) than those patients achieving good outcomes. In the univariable analysis, a lower HIR (OR, 0.008; 95%CI, 0.001–0.254, P = 0.006) was associated with favorable outcome. The volume size of Tmax > 12s was significantly and positively correlated with the size of ischemic core. A HIR value higher than 0.491 might predict a futile recanalization and poor outcome (AUC = 0.696).
Conclusions
The critical hypoperfusion biomarkers on CTP could be useful in triaging endovascular treatment and identifying stroke patients at risk of futile recanalization.
Tumor Volume of Onset and MYCN Amplification Predict Poor Prognosis in Patients with Neuroblastoma
Authors: Ji Fengming , Zhu Jianjun , ZhangHuang Chenghao , Hang Yu , Tang Haoyu , Li Li , Yan Bing 
Published: June 2023

Abstract
Aim: To analysis the prognosis of neuroblastoma (NB) in children.
Method: Clinical and pathological data of NB patients who were diagnosed and treated in Kunming Children's Hospital from January 2016 to December 2022 were retrospective analyzed. The prognostic factors and independent prognostic factors of NB were analyzed by Kaplan-Meier survival analysis and Cox risk ratio model.
Result: According to the inclusion criteria and exclusion criteria, a total of 100 children were included in this study. The 1-year, 3-year and 5-year overall survival (OS) rates were 98.9%, 65.7% and 59.7%, respectively. The prognostic factors of OS were age, metastasis, stage of International Neuroblastoma Staging System (INSS), risk stratification, MYCN, ferritin (Fer), lactate dehydrogenase (LDH), neuron specific enolase (NSE), tumor volume of onset (TVO), image-defined risk factors (IDRFs), preoperative chemotherapy and recurrence. The independent prognostic factors were TVO and MYCN amplification.
Conclusion: TVO and N-myc amplification and MYCN amplification are the independent prognostic factors have predictive value for NB OS prognosis. Patients with TVO ≥751.25 or MYCN amplification have poor prognosis.
Plant-based diet index among male footballers and their non-athlete controls. Is it associated with oxidative stress biomarkers?
Authors: Zare Mahsa , Nouri Mehran , Zare Sahar , Leilami Kimia , Sarbakhsh Parvin , Eftekhari Mohammad Hasan , Gargari Bahram Pourghassem 
Published: June 2023

Abstract
Background
Since well-chosen nutrition exerts a vital role in modulating oxidative status, in addition plant-based diets represent a growing trend among athletes, our purpose was to determine plant-based diet index (PDI) score among male footballers and their non-athlete controls, as well as its association with oxidative biomarkers assessed by urinary excretions of F2alpha-isoprostane (F2a-IP) and 8-hydroxy-2'-deoxyguanosine (8-OHdG).
Methods
A footballer group and a healthy non-athlete group were recruited comprising N = 45 participants each. The two groups were matched individually based on body mass index (BMI) and age. Anthropometric indices and body composition were evaluated, and fasted urine samples were collected to measure oxidative biomarkers. A validated semi quantitative food frequency questionnaire was used to calculate -overall PDI, healthful plant-based diet index (hPDI), and unhealthful plant-based diet index (uPDI). Generalized estimating equation (GEE) model was used for all data analysis.
Results
Compared to the non-athlete group, the PDI score was significantly greater in the footballer group (β = 9.8; P < 0.001), while, the differences between the two groups in uPDI and hPDI scores were not significant. Overall, the consumption of plant-based foods was higher in footballers. On the association of mentioned dietary indices and oxidative biomarkers, only a significant and negative association was shown between PDI scores and F2a-IP levels in all participants (β= -1.33; P = 0.02).
Conclusions
Based on the results, football athletes were more adherent to plant-based diet compared to the non-athletes. Furthermore, it is likely that higher PDI may be useful in lowering oxidative stress with decreased urinary F2a-IP levels.
Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH
Authors: Makai Diána , Mihók Edit , Polgári Dávid , Cseh András , Lenykó-Thegze Andrea , Sepsi Adél , Sági László 
Published: June 2023

Abstract
Background Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situdetection of proteins and nucleic acids are still sporadic in plant cell biology. A major bottleneck has been the availability of high-quality plant nuclei with a balance between preservation of 3D ultrastructure and maintaining immunoreactivity.
The aim of this study was to develop a quick and reliable procedure to prepare plant nuclei suitable for various combinations of immunolabelling and fluorescence in situ hybridisation methods (immunoFISH-GISH).
Results The mechanical removal of the cell wall and cytoplasm, instead of enzymatic degradation, resulted in a gentle, yet effective, cell permeabilisation. Rather than manually releasing the nuclei from the fixed tissues, the procedure involves in-solution cell handling throughout the fixation and the preparation steps as ended with pipetting the pure nuclei suspension onto microscope slides. The optimisation of several critical steps is described in detail. Finally, the procedure is shown to be compatible with immunolabelling, FISH and GISH as well as their simultaneous combinations.
Conclusion A simple plant cell nuclei preparation procedure was developed for combined immunolabelling-in situ hybridisation methods. The main and critical elements of the procedure are: a short period of fixation, incorporation of detergents to facilitate the fixation of tissues and the penetration of probes, tissue grinding to eliminate unwanted cell components, and an optimal buffer to handle nuclei. The procedure is time efficient and is easily transferable without prior expertise.
Diclofenac Sodium Nanomedicine Results in Pain-relief and Differential Expression of the RNA Transcriptome in the Spinal Cord of Spared Nerve Injury Rats
Authors: Zhang Yan , YANG DONG , Shuai Bo , Ding Hui , Yang Jinghan , Wang Jia , TANG LI , Yao Shanglong , Zhang Yan 
Published: June 2023

Abstract
Neuropathic pain is chronic pain caused by a lesion or disease of the somatosensory nervous system. Neuropathic pain, with high incidence and complex pathogenesis, is one of the hot spots in clinical medicine and basic research. Currently, prescribed treatments are still unsatisfactory or have limited effectiveness. A medicinal preparation is required that relieves the neuropathic pain and prolongs action time, which has not been discovered. In this study, MIL-101 (Fe) was used to prepare as a drug carrier to control the release of diclofenac sodium, thus achieving the effect of analgesic and sustained release. The release curves revealed that diclofenac sodium could be consecutively released from MIL-101 (Fe) for more than 48 hours. There was no toxicity in vitro and in vivo, and the safety of MIL-101 (Fe) is confirmed by hematoxylin and eosin (HE) and ELISA tests in vivo. The results of behavioral testing, pharmacokinetics, and RNA sequencing analysis showed that MIL-101 (Fe) loaded with diclofenac sodium could improve the mechanical withdrawal threshold (MWT) and cold allodynia induced by SNI, extending the work time for three days. The results indicated that MIL-101 (Fe) possessed good biocompatibility, and the MIL-101 (Fe)-DS takes on analgesic and controlled-release effects, which provides a scientific basis for the clinical treatment of neuropathic pain and the preparation of a new formulation.
Application of ID3 Algorithm in Fault Diagnosis of Continuously Variable Transmission
Authors: huang he , ouyang lifang 
Published: June 2023

Abstract
The technical performance of modern cars is becoming better and better, and the automobile gearbox plays an important role in which. For instance, the use of a Continuously Variable Transmission (CVT), an automatic transmission that can change seamlessly through a continuous range of effective gear ratios, makes the driving more comfortable and consistent. But it is also disadvantageous in that the transmission belt of the CVT is easily damaged. In order to diagnose CVT faults in time and effectively, data mining technology is introduced in. Decision tree classifier is a kind of classification algorithm based on examples, which is widely used all over the world. This paper is based on the data set of the fault diagnosis of CVT, and the ID3 algorithm is used to build the decision tree. Then the rules are extracted to help judge the fault diagnosis, so as to ensure the normal operation of CVT.
A Novel Approach to Perform Linear Discriminant Analyses for a 4-way Alzheimer's Disease Diagnosis Based on an Integration of Pearson’s Correlation Coefficients and Empirical Cumulative Distribution Function
Authors: Mabrouk Besma , Hamida Ahmed Ben , Mabrouki Noura , Bouzidi Nouha , Mhiri Chokri 
Published: June 2023

Abstract
The diagnosis of Alzheimer's disease (AD) in his prodromal stage is a major topic. About 50% of the well-known Mild Cognitive Impairment (MCI) cohort are estimated to develop AD, and the reasons for conversion or not are still unknown. An efficient diagnosis of the earlier (EMCI) and later (LMCI) cohorts among normal control subjects (CN) is still a big research challenge. Encouraged by the available data provided by the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database, we aim to develop a 4-way framework for multi-class diagnosis. One of the standard methods for supervised classification is Linear Discriminant Analysis (LDA). In contrast to the traditional method based on Principal Component Analysis (PCA) followed by LDA, the current paper proposes a novel approach where optimal LDA subspace is integrated with Pearson’s correlation coefficient (PCC) method to overcome the singularity problem resulting in the case of an underdetermined dataset. We choose, for innovation, to operate the brain connectivity reconstructed from diffusion-weighted imaging modality. First, Diffusion Tensor and Magnetic Resonance brain images of 229 subjects have been preprocessed, their correspondent brain connectivity maps have been required, and connections inside and between hemispheres have been extracted. Second, correlation coefficients between features and classes have been determined, and empirical cumulative distribution functions have been reconstructed (ECDF). Features concerned by the transformation into the LDA space are those exhibiting a cumulative frequency above a determined percentile of the ECDF, in condition to guarantee the non-singularity of the within-class variance matrix. Finally, different machine learning algorithms have been performed and evaluated thanks to the repeated five-fold Cross-Validation procedure. Compared to other methods, the originality of this work is that an accuracy of 100% has been achieved for the LMCI class diagnosis. Furthermore, the connectivity between hemispheres has been identified as a biomarker for disease diagnosis.
Short-term outcomes after laparoscopic colorectal surgery in patients over 90 years old: a Japanese multicenter study
Authors: Yamashita Mariko , Tominaga Tetsuro , Nonaka Takashi , Hisanaga Makoto , Takeshita Hiroaki , Fukuoka Hidetoshi , To Kazuo , Tanaka Kenji , Sawai Terumitsu , Nagayasu Takeshi 
Published: June 2023

Abstract
Background
The effect of laparoscopic surgery on short-term outcomes in colorectal cancer patients over 90 years old were still unknown.
Methods
We reviewed 87 colorectal cancer patients aged over 90 years who underwent surgery between 2016 and 2022. Patients were divided into an OP group (n = 22) that underwent open surgery and a LAP group (n = 65) that underwent laparoscopic surgery. Clinicopathological features were compared between groups.
Results
Seventy-eight patients (89.7%) had comorbidities. Frequency of advanced T stage was lower with laparoscopic surgery (p = 0.021). Operation time was longer (OP 146 min vs. LAP 203 min; p = 0.002) and blood loss was less (105 mL vs. 20 mL, p < 0.001) with laparoscopic surgery. Length of hospitalization was longer with open surgery (22 days vs. 18 days; p = 0.007). Frequency of infectious complications was lower with laparoscopic surgery (18.5%) than with open surgery (45.5%; p = 0.021). Multivariate analysis revealed open surgery (P = 0.026; odds ratio, 3.535; 95% confidence interval, 1.159–10.781) as an independent predictor of postoperative infectious complications.
Conclusions
Laparoscopic colorectal resection for patients over 90 years old is useful procedure that reduces postoperative infectious complications.
Carotid Endarterectomy using Regional Anesthesia: Technique and Considerations.
Authors: Padmanaban Varun , Caldwell Catherine , Milne Indigo , Hazard Sprague W. , Harbaugh Robert E. , Church Ephraim W. 
Published: June 2023

Abstract

BACKGROUND
Carotid endarterectomy (CEA) is one of the most effective neurosurgical operations in minimizing stroke risk in both symptomatic and asymptomatic patients with carotid stenosis. Awake CEA with regional anesthesia may decrease both perioperative complications and length of hospital stay. The awake carotid operation is not often described in published literature.
OBJECTIVE
To describe our experience with carotid endarterectomy using regional anesthesia with a focus on patient selection, anatomic variations, and surgical technique including cervical regional block. We particularly focus on nuances of the awake approach.
METHODS
Carotid endarterectomy using regional anesthesia is described in detail.
RESULTS
Successful use of regional anesthesia during carotid endarterectomy without complication.
CONCLUSION
Regional anesthesia for CEA is an advantageous approach for cervical plaque removal in appropriate patients. Thoughtful patient selection, as well as understanding of anatomy and its variants, is required. Potential advantages and disadvantages are discussed.
Proving Linearizability Using Linearizations of Delete Operations
Authors: Wen Tangliu , Peng Jie , Song Lan 
Published: June 2023

Abstract
Linearizability is a commonly accepted correctness criterion for concurrent data structures. Concurrent queues and stacks are among themost fundamental concurrent data structures. In this paper, we presentnecessary and sufficient conditions for proving linearizability of concurrent queues and stacks, which make use of linearizations of dequeueand pop operations. The verification conditions intuitively express the FIFO/LIFO semantics of concurrent queues/stacks and can be verified just by reasoning about the happened-before order of operations. Wehave successfully applied the proof technique to prove 9 challenging concurrent queues and stacks and optimize the TS queue. We believe thatour proof technique can be extended to the concurrent data structureswhich have the ordering requirements when their elements are removed.
A Critical Evaluation of Pictogram-Based Patient Information Leaflet on Hypothyroidism Patients with Metabolic Syndrome: A Visual Approach to Enhance Health Literacy
Authors: Shaji Bipin , Joel Juno Jerold , Sharma Raghava , Shetty Shraddha , Shastry C. S. , Mateti UdayVenkat 
Published: June 2023

Abstract
Hypothyroidism is a condition with an underactive thyroid gland. Since thyroid hormones play a major role in metabolism, hypothyroidism is often associated with metabolic syndrome.Thus, the patient’s awareness regarding metabolic syndrome is crucial.Considering this background, we intended to develop a Pictogram-based Patient Information Leaflet (P-PIL) for hypothyroidism with metabolic syndrome to provide concise patient education. The developed leaflet was evaluated for its accuracy and reliability. This is a quasi-experimental study without a control group. The leaflet's FleschReading Ease (FRE) and Flesch-Kincaid Grade Level (FKGL) scores were 62.0 and 7.1 respectively. The Baker Able Leaflet Design (BALD) index of English, Kannada, and Malayalam versions of the P-PIL were 28, 27, and 27 respectively. The user testing of the P-PIL was assessed in 72 patients. The overall mean knowledge assessment scores showed a significant improvement from 52.92 ± 6.90 to 77.92 ± 9.31.The majority of the patients, specifically 84.72%, had an opinion that the design and layout of the P-PIL is good.The evaluation results strongly suggest that this P-PIL can be an effective educational tool for hypothyroidism patients with metabolic syndrome.
Applications of CRISPR Cas-9 in Ovarian Cancer Research
Authors: Sherazi Syed Aoun Mehmood , Rafique Fareena , Haris Muhammad , Arshad Abida , Qaiser Hammad , Uzair Mohammad , Arshad Muhammad 
Published: June 2023

Abstract:
Ovarian cancer is a highly prevalent malignancy among women and affects a significant
population worldwide. Different forms of hormonal treatments or chemotherapies are used to treat
ovarian cancer, but the possible side effects, including menopausal symptoms, can be severe, forcing
some patients to prematurely stop the treatment. The emerging genome editing technology, known as
clustered regularly interspaced short palindromic repeats (CRISPR)-caspase 9 (Cas9), has the potential
to treat ovarian cancer via gene editing strategies. Studies have reported CRISPR knockouts of several
oncogenes that are involved in the pathogenesis of ovarian cancer, such as BMI1, CXCR2, MTF1,
miR-21, and BIRC5, and demonstrate the potential of the CRISPR-Cas9 genome editing technique to
effectively treat ovarian cancer. However, there are limitations that restrict the biomedical applications
of CRISPR-Cas9 and limit the implementation of Gene therapy for ovarian cancer. These include offtarget
DNA cleavage and the effects of CRISPR-Cas9 in non-target, normal cells. This article aims to
review the current state of ovarian cancer research, highlight the significance of CRISPR-Cas9 in
ovarian cancer treatment, and establish the groundwork for potential clinical research.
Application of Computed Tomography Perfusion Imaging-guided Mechanical
Thrombectomy in Ischemic Stroke Patients with Large Vessel Occlusion beyond
the Therapeutic Time Window
Authors: Xiang Shifeng , Su Ya , Li Shuyuan , Yang Sujun , Wu Yiping 
Published: June 2023

Introduction:
To explore the feasibility of applying computed tomography perfusion (CTP) imaging-guided mechanical thrombectomy in acute ischemic stroke patients with large vessel occlusion beyond the therapeutic time window.

Methods:
The clinical data of acute cerebral infarction patients with large vessel occlusion who were beyond the therapeutic time window and admitted to Handan Central Hospital from January 2021 to March 2022 were retrospectively analyzed. All patients were assessed by the National Institutes of Health Stroke Scale (NIHSS) and were examined by one-stop CTP imaging. The preoperative onset time of the disease was more than 6 h. Fourteen patients underwent magnetic resonance imaging examination at the same time. Fifty-four patients were retrospectively divided into two groups based on the treatment methods: the mechanical thrombectomy group had 21 patients and the conservative treatment group had 33 patients. NIHSS scoring and computed tomography scan were performed before treatment, 6 h, 24 h, 7 days, and 30 days after treatment.

Results:
The NIHSS scores of the patients with acute cerebral large vessel occlusion who underwent CTP imaging-guided mechanical thrombectomy at 6 h, 24 h, 7 days, and 30 days after treatment were compared with those of the conventional treatment group. The NIHSS score of the mechanical thrombectomy group was significantly better, and the difference was statistically significant (P < 0.05). In terms of the prognosis rate and expansion rate of infarct core volume, the patients of the mechanical thrombectomy group had a better prognosis, and the difference was statistically significant (P < 0.05). Artificial intelligence-assisted CTP diagnosis can facilitate the automatic evaluation of diseases and enable quick judgments that are independent of radiologists evaluation, but it may pose a problem in the determination of infarct core volume (either being too high or too low).

Conclusion:
It is of great significance to apply CTP imaging in guiding the mechanical thrombectomy procedure in acute stroke patients with large vessel occlusion who are beyond the therapeutic time window.
Genotoxic and Mutagenic Assessment of PT-31, a Molecule with
Antipsychotic Potential
Authors: Bigolin Cassiana , Veiverberg Andriele , Prado Rodrigues Gabriela Zimmermann , Hilario Garcia Ana Letícia , Machado Kayser Juliana , Bertoldi Fernando , Dutra Arbo Marcelo , Galdino Pitta Marina , da Rocha Pitta Ivan , Gehlen Günther , Heemann Betti Andresa 
Published: June 2023

Abstract:
The PT-31 molecule, a potential antipsychotic, has demonstrated promising results when orally administrated to in vivo models. A recent study suggested the genotoxic and mutagenic po-tential of PT-31 after acute treatment by intraperitoneal route. This study aimed to evaluate PT-31 potential of inducing genotoxic or mutagenic damage after acute oral administration. For that, adult males and females Balb/C mice were treated acutely by oral administration with vehicle or PT-31 in three different doses (10, 20, and 40 mg kg-1). After 24 hours from PT-31 administration, animals were euthanized for performing the comet and micronucleus assays. None of the tested groups of PT-31 presented a significant increase in damage index and MN frequency. However, they presen-ted the following tendency on damage index: females presented a tendency at 40 mg kg-1 and males at 20 mg kg-1. Regarding the MN assay, male mice at the highest dose of 40 mg kg-1 presented a tendency of increased MN frequency. Also, there was a significant increase in PCE/NCE ratio in male mice. Results suggest that the male mice group presented higher susceptibility to damage. The tendency of increased damage to DNA and MN frequency suggests that the molecule PT-31 may induce reparable damage to DNA, and these DNA strand repairs may have originated from the MN. However, significant genotoxic and mutagenic effects were not observed. This study reinforces the atypical profile of the molecule as much as its safety by oral route administration.