bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of non-parametric approaches to identify trends in streamflow for the Mae Klong River Basin, Thailand
Authors: Khalil Alamgir 
Published: May 2023

AbstractMean monthly streamflow records of ten gauging stations in the Mae Klong River Basin, Thailand, were analyzed for trends using non-parametric approaches for the period 2000–2015. The statistical significance of trends was tested on monthly, seasonal, and annual scales using three non-parametric tests; Mann–Kendall (MK), Spearman's rho (SR), and innovative trend analysis (ITA) on a 5% significance level. The streamflow stations were grouped into two categories: naturalized streamflow (four stations) and regulated streamflow (six stations). In both the dry and wet seasons, decreasing trends were observed in the naturalized inflows to the Srinagarind and Vajiralongkorn Dams. On an annual basis, all the naturalized streamflow stations showed decreasing trends. For the regulated streamflow stations, in the dry season, four out of six stations showed increasing trends, while in the wet season, five out of six stations indicated decreasing trends. On an annual basis, five stations showed decreasing trends by both the MK and SR tests, while the ITA method indicated decreasing trends for four regulated streamflow stations. The results of this study can help water resources managers for better assessment and planning of water resources in the basin.
Essential Trace Elements in Patients with Dyslipidemia: A
Meta-analysis
Authors: Li Cui-Ping , Song Yu-Xin , Lin Zi-Jun , Ma Mei-Lin , He Lian-Ping 
Published: May 2023

Background:
Lipid metabolism is a complex process that includes lipid uptake,
transport, synthesis, and degradation. Trace elements are vital in maintaining normal
lipid metabolism in the human body. This study explores the relationship between
serum trace elements and lipid metabolism.Lipid metabolism is a complex process that includes lipid uptake,
transport, synthesis, and degradation. Trace elements are vital in maintaining normal
lipid metabolism in the human body. This study explores the relationship between
serum trace elements and lipid metabolism.

Methods:
In this study, we reviewed articles on the relationship between alterations in somatic
levels of zinc, iron, calcium, copper, chrome, manganese, selenium, and lipid
metabolism. In this systematic review and mate-analysis, databases such as PubMed,
Web of Science, and China National Knowledge Infrastructure (CNKI), Wanfang was
searched for articles on the relationship published between January 1, 1900, and July 12,
2022. The meta-analysis was performed using Review Manager5.3 (Cochrane Collaboration).

Results:
No significant association was found between serum zinc and dyslipidemia,
while other serum trace elements (iron, selenium, copper, chromium, and manganese)
were associated with hyperlipidemia.

Conclusion:
The present study suggested that the human body's zinc, copper, and calcium
content may be related to lipid metabolism. However, findings on lipid metabolism
and Iron, Manganese have not been conclusive. In addition, the relationship between
lipid metabolism disorders and selenium levels still needs to be further studied. Further
research is needed on treating lipid metabolism diseases by changing trace elements.
A Review on Artificial Intelligence Approaches and Rational Approaches in Drug
Discovery
Authors: Srivathsa Anjana Vidya , Sadashivappa Nandini Markuli , Hegde Apeksha Krishnamurthy , Radha Srimathi , Mahesh Agasa Ramu , Ammunje Damodar Nayak , Sen Debanjan , Theivendren Panneerselvam , Govindaraj Saravanan , Kunjiappan Selvaraj , Pavadai Parasuraman 
Published: May 2023

Abstract:
Artificial intelligence (AI) speeds up the drug development process and reduces its time, as well as
the cost which is of enormous importance in outbreaks such as COVID-19. It uses a set of machine learning algorithms
that collects the available data from resources, categorises, processes and develops novel learning
methodologies. Virtual screening is a successful application of AI, which is used in screening huge drug-like
databases and filtering to a small number of compounds. The brain’s thinking of AI is its neural networking
which uses techniques such as Convoluted Neural Network (CNN), Recursive Neural Network (RNN) or Generative
Adversial Neural Network (GANN). The application ranges from small molecule drug discovery to the
development of vaccines. In the present review article, we discussed various techniques of drug design, structure
and ligand-based, pharmacokinetics and toxicity prediction using AI. The rapid phase of discovery is the
need of the hour and AI is a targeted approach to achieve this.
Folate Targeting Peptide Conjugates for Inflammatory Response Suppression
Authors: Ruff Elizabeth , Poh Scott 
Published: May 2023

Background and Objective:
Protein kinases known as mitogen-activated protein kinases (MAPKs) are
responsible for regulating a wide variety of physiological cell responses by generating and release of inflammatory
mediators. Suppressing these inflammatory mediators can be utilized to control the propagation of inflammation.
During the course of this research, we created folate-targeted MK2 inhibitor conjugates and analyzed the antiinflammatory
effects of these compounds.

Methods:
Using RAW264.7 cells, which are generated from murine macrophages, as an in vitro model. We synthesize
and evaluated a folate linked peptide MK2 inhibitor. The cytotoxicity was assessed using the ELISA kits, CCK-
8 test kit, NO concentration and inflammatory factors TNF-, IL-1, and IL-6.

Results:
The cytotoxicity assay results suggested that the concentration for MK2 inhibitors less than 50.0 μM be
non-toxic. The ELISA Kits also demonstrated that MK2 peptide inhibitor treatment significantly decreased the content
of NO, TNF-, IL-1, and IL-6 in LPS-stimulated RAW264.7 cells. It was also demonstrated that a folate-targeted
MK2 inhibitor was more effective than a non-targeted inhibitor.

Conclusion:
This experiment demonstrates that LPS-induced macrophages can produce oxidative stress and inflammatory
mediators. According to our research, pro-inflammatory mediators can be reduced by targeting folate receptor-
positive (FR+) macrophages with an FR-linked anti-inflammatory MK2 peptide inhibitor in vitro, and the uptake
was FR-specific.
Smoking Affects the Predictive Roles of Antioxidant Enzymes in the
Clinical Response to Risperidone in Schizophrenia: A Large-scale Cohort
Study
Authors: Xiu Meihong , Song Xiuli , Yang Hanlun , Huang Xingjuan , Wu Fengchun , Zhang Xiangyang 
Published: May 2023

Objectives:
There is overwhelming evidence of the relationship between smoking and
schizophrenia (SZ). Tobacco smoke is considered to ameliorate the symptoms and reduce the side effects
of antipsychotics in SZ patients. However, the underlying biological mechanism by which tobacco
smoke improves symptoms in SZ remains unclear. This study was designed to examine the effects
of tobacco smoke on antioxidant enzyme activities and psychiatric symptoms after receiving 12-week
risperidone monotherapy.

Methods:
Two hundred and fifteen antipsychotic-naïve first-episode (ANFE) patients were recruited
and treated with risperidone for 3 months. The severity of the patient’s symptoms was assessed by the
Positive and Negative Syndrome Scale (PANSS) at baseline and at post-treatment. Plasma SOD,
GSH-Px, and CAT activities were determined at baseline and follow-up.

Results:
Relative to nonsmoking patients with ANFE SZ, patients who smoked had higher baseline
CAT activity. In addition, among non-smokers with SZ, baseline GSH-Px was associated with clinical
symptom improvement, while baseline CAT was associated with positive symptom improvement in
smokers with SZ.

Conclusion:
Our findings demonstrate that smoking affects the predictive role of baseline SOD, GSHPx,
and CAT activities on clinical symptom improvement in patients with SZ.
Catalytic One-pot Solvent Free Synthesis, Biological Activity, and Docking
Study of New Series of 1, 3-thiazolidine-4-one Derivatives Derived from 2-
(P-tolyl) Benzoxazol-5-amine
Authors: Khudhur Hussein Kaka Ahmed , Hussein Awaz Jamil 
Published: May 2023

Objective:
In this study, a simple triethylammonium salt of phosphoric acid (tri-ethylammonium dihydrogen phosphate) (4) in the liquid state was utilized as an inexpensive, effi-cient one-pot three components, solvent-free synthesis of thiazolidine-4-one derivatives, with good to excellent yields. Techniques such as FT-IR, 1H-NMR, 13C-NMR, 13C-NMR-DEPT-135, and MS. were used for the structural elucidation. The high biotic efficiency of the newly obtained compounds was confirmed by in vitro antimicrobial action against Gram-positive (S. Aureus), Gram-negative bacteria (P. Aeruginosa and E. Coli) and antifungal activity (C. Albicans) via mi-croplate titer dilution technique. Finally, a molecular docking study was performed with a re-solved crystal structure of S. Aureus D-alanine alanyl carrier protein ligase (PDB ID: 7VHV). This investigation aimed to synthesize a new series of thiazolidine-4-one derivatives combined with benzoxazole moiety.

Materials and Methods:
Ionic liquid assistance one-pot solvent-free synthesis method used to synthesize a new series of thiazolidine-4-one derivative 10(a-e).

Results:
Structural identification of new synthesis and biological evaluation via techniques of (IR, 1H-NMR, 13C-NMR, 13C-NMR-DEPT-135, and MS).

Conclusion:
Ionic liquid is utilized as an inexpensive, efficient one-pot three-component solventfree
synthesis of thiazolidine-4-one derivatives with good to excellent yields.

:
Most of the synthesized compounds showed high biological and anti-fungal activity, in line with
the docking study against mentioned microorganism and crystal structure of PDB (ID: 7VHV), respectively.
Design and In-silico Screening of Short Antimicrobial Peptides (AMPs) as
Anti-Tubercular Agents Targeting INHA
Authors: Mehta Kanchan , Vyas Pallavi , Mujawar Shama , Hazam Prakash Kishore , Vyas Ashish 
Published: May 2023

Introduction:
Tuberculosis has been studied as a potentially serious bacterial infection affecting
the lung caused by Mycobacterium tuberculosis. In addition to its severe impact on health, resistance
to existing drugs has also been seen as a rising concern in the space of medicinal solutions.
Therapeutic peptides have the potential to complement existing drug designs to provide effective outcomes
against Mycobacterium tuberculosis-resistant strains.

Methods:
This study illustrated a computational approach to design and test peptides against NADHdependent
enoyl-acyl carrier protein reductase of Mycobacterium tuberculosis. A human antimicrobial
peptide LL-37 was used as a template, and a further 6 peptides were designed, and their binding and interactions
against NADH-dependent enoyl-acyl carrier protein reductase were examined. Further, toxicity,
immunogenicity, and a broad spectrum of physicochemical properties were calculated to evaluate
the therapeutic and safety profile of these peptides.

Results:
These peptides were structurally modelled and docked with the protein to determine their binding
poses and affinity. The molecular interaction of LL-37 with protein was treated as a reference to
evaluate the effectiveness of designed peptides. Solvent accessible surface area (SASA) and ΔG binding
free energy of docked complexes assisted in the ranking of these peptides. Eventually, peptides P1:
LLGDFFRKSKEK, P3: LLFGDRFLLKEK and P7: LLGDFFRLLKEK were selected for 100 ns molecular
dynamic simulation as they showed predicted dissociation constants of 8.7×10-4 M, 3.3×10-4 M
and 1.2×10-4 M, respectively. These peptides showed direct hydrogen bond formation with ILE21 and
LYS165, which are critical active site residues of the protein. The structural variation pattern collected
from the MD simulation suggested a strong and stable binding of P3 and P1 with the protein with
RMSD 4-5 Å with the starting conformation under the non-fluctuating state. These two peptides showed
relatively similar binding results compared with the control peptide LL-37. Comprehensive structural
analysis was performed for the middle structures of the most populated cluster generated from 100 ns
MD simulation trajectory.

Conclusion:
Later, MMPBSA binding energies of these structures were computed, where the average
binding free energies of P1, P3, and P7 peptides were -146.93 kcal/mole, -161.16 kcal/mole, and -
151.44 kcal/mole, respectively. These energies suggested that P3 is strongly bound to the active site of
NADH-dependent enoyl-acyl carrier protein reductase. Overall, this study proposed the application of
these peptides as a possible therapeutic solution to inhibit the growth of Mycobacterium tuberculosis.
Anti-inflammatory Activity: In silico and In vivo of Sapogenins Present in Agave brittoniana subsp. brachypus (Trel.)
Authors: Resino-Ruiz Dayana , Gonzalez-Madariaga Yisel , Nieto Leisy , Linares Yilka Mena , Guerra León Jose Orestes , Martín Arlena Vázquez , Díaz Arianna Valido , Torrens Francisco , Castillo-Garit Juan A. 
Published: May 2023

Background:
Agave brittoniana subsp. brachypus is an endemic plant of Cuba, which contains different steroidal sapogenins with anti-inflammatory effects. This work aims to develop computational models which allow the identification of new chemical compounds with potential anti-inflammatory activity.

Methods:
The in vivo anti-inflammatory activity was evaluated in two rat models: carrageenan-induced paw edema and cotton pellet-induced granuloma. In each study, we used 30 Sprague Dawley male rats divided into five groups containing six animals. The products isolated and administrated were fraction rich in yuccagenin and sapogenins crude.

Results:
The obtained model, based on a classification tree, showed an accuracy value of 86.97% for the training set. Seven compounds (saponins and sapogenins) were identified as potential anti-inflammatory agents in the virtual screening. According to in vivo studies, the yuccagenin-rich fraction was the greater inhibitor of the evaluated product from Agave.

Conclusion:
The evaluated metabolites of the Agave brittoniana subsp. Brachypus showed an interesting anti-inflammatory effect.
Clinical Dilemma, Bernard Soulier Syndrome versus Immune Thrombocytopenic
Purpura: A Case Report
Authors: J Bhavya , Patel Nitish Kumar , Kumar Nilesh , Singh Jitendra , Kumar Kailash 
Published: May 2023

Background:
Bernard Soulier Syndrome (BSS) is a rare autosomal recessive disorder due
to deficiency or dysfunction of the glycoprotein GPIb-V-IX complex on the platelet surface. It is
also known as hemorrhagiparous thrombocytic dystrophy or congenital hemorrhagiparous thrombocytic
dystrophy. The patient usually presents with severe and prolonged bleeding along with characteristics
of giant blood platelets and low platelet counts. Manifestations of BSS include epistaxis,
gum bleeding, purpuric rashes, menorrhagia, rarely melena, and hematemesis. On the other hand,
immune thrombocytopenic purpura (ITP) is an acquired autoimmune disorder in which there is accelerated
platelet destruction and reduced platelet production. Isolated thrombocytopenia without
fever, lymphadenopathy, and organomegaly usually lead to the diagnosis of immune thrombocytopenia.

Case Presentation:
A 20 years old female presented with complaints of recurrent episodes of epistaxis
since childhood and menorrhagia during menarche. She was misdiagnosed as ITP elsewhere.
Later, based on thorough clinical examination and investigation, the diagnosis was confirmed as
BSS.

Conclusion:
BSS should always be taken in the differential diagnosis of ITP, especially when persistent,
refractory, and treated unsuccessfully with steroids or splenectomy.
The Effect of the Seed of Descurainia Sophia on Functional Constipation
in Iranian Women Aged 50-70 Years: A Randomized Controlled Trial
Authors: Tabatabaeichehr Mahbubeh , Mortazavi Hamed , Pournaghi Seyed-Javad , Kasaian Jamal , Yaaghoobian Barmak , Akbari Hadi , Kheivegi Neda , Alesheikh Peiman 
Published: May 2023

Background:
Constipation in older adults is a common issue and is more prevalent in
women. The current study aimed to assess the effectiveness of the seed of Descurainia Sophia as
an herbal medicine on the severity of constipation in older women.

Methods:
Total, sixty-four women aged between 50 to 70 participated in the study. The study used
a randomized controlled trial to investigate the effectiveness of the seed of Descurainia Sophia on
constipation. Data were collected using a constipation assessment scale and analyzed using independent
samples t-test and gain score procedure.

Results:
It was shown that taking a mixture of water and seeds of Descurainia Sophia significantly
reduces constipation severity in older women.

Conclusion:
It was concluded that the seed of Descurainia Sophia is a safe and effective alternative
for treating constipation in older women.
Preparation of Encapsulated Turmeric Extract for Food Fortification
Authors: Uslu Mehmet Emin , Öder Gizem , Bayraktar Oguz 
Published: May 2023

Introduction:
Cross-linked alginate beads containing turmeric extract were produced using the electrospraying method to achieve the release of bioactive compounds in a sustained manner.

Methods:
An aqueous alginate solution containing turmeric extract was electrosprayed into an aqueous phase containing a crosslinking agent (calcium chloride) at different process variables (alginate concentrations, flow rates and voltages). Then, particle size and distribution were investigated with an optical microscope.

Results:
Prepared alginate beads with 1 wt % alginate concentration, 20 kV and 1 ml/h exhibited the desired beads with turmeric extract content. Alginate beads containing turmeric extract were kept in water for 72 hours and total amount of antioxidant and total amount of phenol which was released to liquid were investigated. Afterwards, solution with alginate beads were centrifuged and dried to observe structural changes. Total antioxidant activity and total phenol in the dried beads were studied. Some samples released most amount of antioxidants and phenol to the medium. Similarly, some samples kept all antioxidants and phenol.

Conclusion:
In conclusion, electroencapsulation can be used in food applications in order to con-serve the bioactivity of active compounds.
Metacognitive skills of student in solving problems of two-variable linear equation systems in terms of self-regulated learning
Authors: Rahmawati Erlina Zulva , Setyaningsih Nining 
Published: May 2023

It is empirically known that metacognitive skill contributes to self-regulated learning. This study aims to describe students’ metacognitive skills in three levels of self-regulated learning, particularly in solving problems of a two-variable linear equation system. Three indicators were used in metacognition skills, namely planning indicators, monitoring indicators, and evaluation indicators. Employing a descriptive qualitative approach, this study involved All students of grade VIII A of the academic year 2021/2022 from a state junior school as subjects involving each category for high, medium, and low self-regulated learning. The data were collected using questionnaires, tests, interviews, documentation, and observation. A triangulation method, The data were then analyzed through condensation, data presentation, and drawing conclusions. is the current study found that high learning students used their metacognitive skills well and met three indicators, namely planning indicators, monitoring indicators, and evaluation indicators. Students with moderate self-regulated learning were able to meet two indicators, including planning indicators and monitoring indicators, while those with low self-regulated learning only met planning indicators.
Ontarians’ Perceptions of Public Health Communications and Misinformation During the COVID-19 Pandemic: Survey Study
Authors: Fahim Christine , Cooper Jeanette , Theivendrampillai Suvabna , Pham Ba' , Straus Sharon 
Published: May 2023

Background
Clear, accurate, and transparent risk communication is critical to providing policy makers and the public with directions to effectively implement public health strategies during a health emergency.

Objective
We aimed to explore the public’s preferred sources of obtaining COVID-19 information, perceptions on the prevalence and drivers of misinformation during the pandemic, and suggestions to optimize health communications during future public health emergencies.

Methods
We administered a web-based survey that included Likert scale, multiple choice and open-ended response questions to residents of Ontario, Canada. We aimed to recruit a sample that reflected population diversity with respect to age and gender. Data were collected between June 10, 2020, and December 31, 2020, and were analyzed using descriptive statistics; open-ended data were analyzed using content analysis. Subgroup analyses to explore perceptions by age and gender were conducted using ordinal regression.

Results
A total of 1823 individuals participated in the survey (n=990, 54% women; n=703, 39% men; n=982, 54% aged 18-40 years; n=518, 28% aged 41-60 years; and n=215, 12% aged ≥61 years). Participants most commonly obtained COVID-19 information from local television news (n=1118, 61%) followed by social media (n=938, 51%), national or international television news (n=888, 49%), and friends and family (n=835, 46%). Approximately 55% (n=1010) of the participants believed they had encountered COVID-19–related misinformation; 70% (n=1284) of the participants reported high levels of trust in health authority websites and health care providers; 66% (n=1211) reported high levels of trust in health ministers or public health organizations. Sources perceived to be less trustworthy included friends and family, talk radio, social media, as well as blogs and opinion websites. Men were more likely to report encountering misinformation and to trust friends or family (odds ratio [OR] 1.49, 95% CI 1.24-1.79) and blogs or opinion websites (OR 1.24, 95% CI 1.03-1.50), compared to women. Compared to those aged 18-40 years, participants aged ≥41years were more likely to trust all assessed information sources, with the exception of web-based media sources, and less likely to report encountering misinformation. Of those surveyed, 58% (n=1053) had challenges identifying or appraising COVID-19 information.

Conclusions
Over half of our participants perceived that they had encountered COVID-19 misinformation, and 58% had challenges identifying or appraising COVID-19 information. Gender and age differences in perceptions of misinformation and trust in information sources were observed. Future research to confirm the validity of these perceptions and to explore information-seeking patterns by population subgroups may provide useful insights on how to optimize health communication during public health emergencies.