bluuebunny/crossref_metadata_2025_split huggingface dataset:
The 2‐methylpropene degradation pathway in Mycobacteriaceae family strains
Authors: Helbich Steffen , Barrantes Israel , dos Anjos Borges Luiz Gustavo , Pieper Dietmar H. , Vainshtein Yevhen , Sohn Kai , Engesser Karl‐Heinrich 
Published: June 2023

AbstractMycolicibacterium gadium IBE100 and Mycobacterium paragordonae IBE200 are aerobic, chemoorganoheterotrophic bacteria isolated from activated sludge from a wastewater treatment plant. They use 2‐methylpropene (isobutene, 2‐MP) as the sole source of carbon and energy. Here, we postulate a degradation pathway of 2‐methylpropene derived from whole genome sequencing, differential expression analysis and peptide‐mass fingerprinting. Key genes identified are coding for a 4‐component soluble diiron monooxygenase with epoxidase activity, an epoxide hydrolase, and a 2‐hydroxyisobutyryl‐CoA mutase. In both strains, involved genes are arranged in clusters of 61.0 and 58.5 kbp, respectively, which also contain the genes coding for parts of the aerobic pathway of adenosylcobalamin synthesis. This vitamin is essential for the carbon rearrangement reaction catalysed by the mutase. These findings provide data for the identification of potential 2‐methylpropene degraders.
Caring through things at a distance: Intimacy and presence in teletherapy assemblages
Authors: Arribas‐Ayllon Michael A. M. 
Published: June 2023

AbstractThe COVID‐19 crisis in the UK precipitated a sharp rise in the use of remote technologies to provide therapy during the lockdown. With mental health care services migrating to devices and video‐conferencing platforms, nearly all forms of therapy had become ‘teletherapy’. Drawing on interviews with UK‐based practitioners, this paper explores how existing ideas of intimacy and presence are challenged when care is practiced at a distance. Against the background of concerns that remote technologies erode intimacy and degrade physical presence, the argument is made that presence, distance, intimacy and control are reconfigured within mediated therapy. Analysis of practitioners’ experiences of teletherapy examines the material and expressive components of ‘assemblages’ characterised by their stable and fluid properties. Two assemblages are identified and discussed: emergency care assemblages and assemblages of intimacy, both of which are aligned with specific sectors of mental health care. Evidence that therapeutic encounters are constrained by technologies are considered alongside the material conditions and inequalities of vulnerable groups, while assemblages with relatively stable properties are generative of new ways of relating to clients online. These findings highlight the material and expressive components of human and nonhuman assemblages that create new kinds of affective relations in distanced care.
‘It’s not as good as the face‐to‐face contact’: A sociomaterialist analysis of the use of virtual care among Canadian gay, bisexual and queer men during the COVID‐19 pandemic
Authors: Daroya Emerich , Grey Cornel , Klassen Ben , Lessard David , Skakoon‐Sparling Shayna , Perez‐Brumer Amaya , Adam Barry , Cox Joseph , Lachowsky Nathan J. , Hart Trevor A. , Gervais Jessie , Tan Darrell H. S. , Grace Daniel 
Published: June 2023

AbstractThe COVID‐19 pandemic led to the widespread adoption of virtual care—the use of communication technologies to receive health care at home. We explored the differential impacts of the rapid transition to virtual care during the COVID‐19 pandemic on health‐care access and delivery for gay, bisexual and queer men (GBQM), a population that disproportionately experiences sexual and mental health disparities in Canada. Adopting a sociomaterial theoretical perspective, we analysed 93 semi‐structured interviews with GBQM (n = 93) in Montreal, Toronto and Vancouver, Canada, conducted between November 2020 and February 2021 (n = 42) and June‐October 2021 (n = 51). We focused on explicating how the dynamic relations of humans and non‐humans in everyday virtual care practices have opened or foreclosed different care capacities for GBQM. Our analysis revealed that the rapid expansion and implementation of virtual care during the COVID‐19 pandemic enacted disruptions and challenges while providing benefits to health‐care access among some GBQM. Further, virtual care required participants to change their sociomaterial practices to receive health care effectively, including learning new ways of communicating with providers. Our sociomaterial analysis provides a framework that helps identify what works and what needs to be improved when delivering virtual care to meet the health needs of GBQM and other diverse populations.
Nail analysis for the assessment of caffeine abuse in Northwest China: A population‐based study
Authors: Qiao Hongwei , Shao Litao , Chen Jie , Jiang Shan , Su Mengxiang , Liu Peipei , Di Bin 
Published: June 2023

AbstractCaffeine is the most widely consumed psychoactive agent worldwide and has the potential for abuse, but studies monitoring caffeine abuse in China are scarce. This study aims to estimate the prevalence of caffeine abuse in northwest China and investigate the correlation between caffeine and other drugs in hair and nails using an ultra‐performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS). Fingernail clippings were collected from 376 participants in northwest China to detect caffeine and 13 other illicit psychoactive drugs and their metabolites. Paired hair and nail samples were collected from 39 participants to investigate the correlation between caffeine and other drugs in hair and nails. The samples were decontaminated, pulverized, and extracted by a high‐throughput nail sample preparation method and analyzed by UPLC‐MS/MS. The results showed a risk of caffeine abuse in northwest China, with concentrations ranging from 0.43 to 10.6 ng/mg for healthy volunteers, 0.49–246 ng/mg for caffeine abusers, and 0.25–363 ng/mg for drug addicts in community rehabilitation centers. Caffeine was detected together with other illicit psychoactive drugs and their metabolites. Furthermore, positive detection correlations were found between hair and nail samples. This study provides a current perspective on caffeine abuse in northwest China and demonstrates the practical use of UPLC‐MS/MS for the simultaneous detection of caffeine and 13 illicit psychoactive drugs and their metabolites in hair and nails. The results highlight the potential of nails as a supplementary matrix when hair samples are unavailable and emphasize the need for handling caffeine carefully given its potential for abuse.
Compounds identified in an illicit generic Xanax tablet are the result of a failed synthesis of alprazolam
Authors: Kizzire Koby L. , Langlois Emily , Brown Savannah 
Published: June 2023

AbstractUnintended compounds produced by inexperienced clandestine chemists may present a challenge in laboratories tasked with their identification. In March 2020, an anonymously submitted tablet purchased as a generic form of Xanax was analyzed by Erowid's DrugsData.org. The gas chromatography–mass spectrometry (GC–MS) results publicly released online indicated several unidentified compounds due to a lack of database references at that time. Elucidation by our group indicated the presence of several structurally related compounds that were linked to a failed synthesis of alprazolam. For this case study, a published procedure for the synthesis of alprazolam starting with the chloroacetylation of 2‐amino‐5‐chlorobenzophenone was identified as a potential source of this failure. The procedure was reproduced to identify pitfalls of the methodology and examine its possible link to the illicit tablet. Reaction outcomes were analyzed via GC–MS and compared to the tablet submission data. The major compound in this submission, N‐(2‐benzoyl‐4‐chlorophenyl)‐2‐chloroacetamide, along with several related byproducts were successfully reproduced indicating that the tablet contents potentially stem from a failure to synthesize alprazolam.
Occurrence of pesticide residues in honey from apiaries with incidents of honeybee poisoning in East China and a corresponding risk assessment for honeybees and Chinese consumers
Authors: He Liang , Zhang Jie , Shen Leiding , Ji Xiaofeng , Li Rui 
Published: June 2023

AbstractWe investigated the occurrence of 80 pesticide residues in 96 honey samples from apiaries with honeybee poisoning incidences by liquid chromatography–tandem mass spectrometry and subsequently conducted risk assessments of exposure for in‐hive honeybees and Chinese consumers. Six pesticides were detected with residue concentrations ranging from 0.5 to 130.9 µg/kg. The mean concentrations of acetamiprid, dinotefuran, hexythiazox, propargite, semiamitraz, and carbendazim in positive samples were 7.9 ± 9.1, 5.9 ± 1.7, 3.0 ± 1.6, 44.2 ± 50.0, 9.0 ± 9.4, and 5.5 ± 4.1 µg/kg, respectively. Carbendazim, semiamitraz, and acetamiprid were the major contaminants in honey, with incidences of 99.0%, 93.8%, and 49.0%, respectively. The cooccurrence of pesticides (≥2 pesticides) was detected in 95.9% of the samples, with up to six residual pesticides found in one sample. The HQ (hazard quotient) values of the six pesticides to in‐hive honeybees were from 4.7 × 10−8 to 0.021, less than 1, indicating their acceptable exposure risk to honeybees. In terms of the representative‐case and worst‐case scenarios, the sum of separate HQs of each pesticide yielding an HI (hazard index) ranged from 0.012 to 0.016 for in‐hive worker honeybees and from 0.015 to 0.021 for in‐hive larva honeybees, indicating an overall acceptable potential cumulative risk of multiple pesticides to in‐hive honeybees. Both the %ARfD (acute reference dose) value (0.0001–0.075) and %ADI (acceptable daily intake) value (0.00002–0.0046) of risky pesticides were much less than 100, revealing acceptable risk exposure to risky pesticides via honey consumption for human health. Thus, our results showed that multipesticide residual honey from apiaries with honeybee poisoning incidents in East China was safe for humans and in‐hive honeybees.Practical ApplicationThis analytical approach will be used in detecting multiple pesticide residues in honey and risk assessment for dietary exposure to pesticide residues. It can support various surveillance programs about honey safety and in‐hive honeybee health evaluation.
Evaluation of impacts of climate change on natural and managed wetland basins
Authors: Shin Satbyeol , Her Younggu , Khare Yogesh 
Published: June 2023

AbstractLow floodplain wetlands such as the Western Everglades in South Florida are vulnerable to extreme weather events, and their water quality and ecosystem functions vary greatly depending on changes in water levels and discharges. The future (i.e., the mid and late 21st century) climate is projected to result in increased frequency and magnitude of extreme events, which could negatively affect the hydroecological function of the wetlands. Wetland management practices have commonly been implemented to protect wetlands and their functions, but it is not clear whether the current management practices can still be effective in projected climate change scenarios. The main goal of this study was to evaluate the impacts of climate change on the runoff and total phosphorus (TP) of natural (L28 Gap) and managed (L28) wetland watersheds in the Western Everglades. For the assessment, we employed future climate projections made using 29 general circulation models (GCMs) and the Watershed Assessment Model (WAM), a watershed loading model. The WAM was calibrated and validated for the baseline period (2000–2014), and the bias‐corrected climate projections were incorporated into the model to project the runoff discharge and TP loads for the near‐future (2030–2044) and far‐future (2070–2084) periods in two carbon emission scenarios. The modeling results show that the natural wetland watershed would be more vulnerable to projected climate change than the managed wetland watershed. The impact of projected climate change scenarios on daily runoff and TP loads was modulated by water control facilities and practices in the managed watershed, highlighting the significance of watershed management practices for improved water quality under projected climate change. This study demonstrates how the local natural and managed wetland watersheds distinctly respond to the global‐scale changes and emphasizes the role of water management practices in wetland basins, which are expected to help develop effective climate change adaptation plans for improved sustainability of wetland systems.
Increased glutamate in type 2 diabetes in the Korean population is associated with increased plasminogen levels
Authors: Lee Hyo Jung , Yeom Jeong Won , Yun Ji Ho , Jang Han Byul , Yoo Min‐Gyu , Kim Hyo‐Jin , Koo Soo Kyung , Lee Hye‐Ja 
Published: June 2023

AbstractBackgroundGlutamate is a major neurotransmitter, although it causes cytotoxicity and inflammation in nonneuronal organs. This study aimed to investigate the metabolic disorders in which glutamate, associated with type 2 diabetes onset, is induced in the liver.MethodsAn analysis of Korean community‐based Ansan‐Ansung cohort study data as well as functional research using in vitro and mouse models were performed.ResultsGroups with high plasma glutamate levels (T2, T3) had a significantly increased risk of diabetes incidence after 8 years, compared to the group with relatively low glutamate levels (T1). Analysis of the effect of glutamate on diabetes onset in vitro showed that glutamate induces insulin resistance by increasing glucose‐related protein 78 (GRP78) and phosphoenolpyruvate carboxykinase (PEPCK) expression in SK‐Hep‐1 human liver cells. In addition, three different genes, FRMB4B, PLG, and PARD3, were significantly associated with glutamate and were identified via genome‐wide association studies. Among glutamate‐related genes, plasminogen (PLG) levels were most significantly increased in several environments in which insulin resistance was induced, and was also upregulated by glutamate. Glutamate‐induced increase in PLG in liver cells was caused by metabotropic glutamate receptor 5 activation, and PLG levels were also upregulated after extracellular secretion. Moreover, glutamate increased the expression of plasminogen activator inhibitor‐1 (PAI‐1). Thus, extracellular secreted PLG cannot be converted to plasmin (fibrinolytic enzyme) by increased PAI‐1.ConclusionsIncreased glutamate is closely associated with the development of diabetes, and it may cause metabolic disorders by inhibiting the fibrinolytic system, which plays an important role in determining blood clots, a hallmark of diabetes.
Boron foliar supplementation as a strategy to attenuate drought stress in soybean
Authors: Souza Júnior Paulo César , Marchiori Paulo Eduardo Ribeiro , Hippler Franz Walter Rieger , Bastos Ludmila da Silva , Linhares Paulo Cassio Alves , Peralta Sanchez Marlon Alexander 
Published: June 2023

AbstractDrought is the major abiotic stress that limits growth, development and yield of crops worldwide. In this scenario, mineral nutrients, such as boron (B), have been promising for increasing the tolerance of plants to abiotic stresses because of their physiological roles in plants. We aimed to evaluate the benefits of foliar supplementation of B in either relieving or reducing the physiological damages caused by water stress in soybean (Glycine max L.). A greenhouse trial was carried out in a 2 × 3 factorial scheme, with two water conditions (well‐watered and drought stress) and three doses of B (0, 150 and 300 mg B L−1). Foliar application of B was before inducing the water stress, which remained for 20 days during vegetative stage. Plants were evaluated at three moments, according to the water conditions: maximum stress, rehydration and just before harvesting. The levels of hydrogen peroxide and lipid peroxidation increased in soybean leaves and roots under water stress, resulting in impaired plant growth. However, the foliar supplementation with B before the stress increased activities of the antioxidant enzymes and reduced the levels of stress markers. Furthermore, B applied foliar increased the nutrient concentration in the leaves of plants and stimulated root growth, which resulted in higher harvest index related to yield. The foliar application of B has shown as an alternative management to mitigate the damages caused by drought stress in soybean.
Contact chemoreceptive mate recognition in Cerambyx welensii Küster (Coleoptera: Cerambycidae)
Authors: Sánchez‐Osorio Israel , López‐Pantoja Gloria , Domínguez Luis , López‐Manzano María R. , Rosell Gloria , Guerrero Ángel 
Published: June 2023

Abstract
Cerambyx welensii Küster (Coleoptera: Cerambycidae) is a wood‐borer responsible for the decline of Mediterranean oaks in open woodlands.
To establish that contact pheromones are involved in mate recognition of C. welensii, we extracted the cuticular hydrocarbons by solid phase microextraction at pre‐reproductive, beginning and ending of the reproductive period, and by solvent extraction of prothorax and elytra. The extracts were analysed by GC–MS under electron impact and chemical ionization conditions. Cuticular hydrocarbon profiles varied according to the reproductive period, although differences between sexes were not significant. Two compounds, 11/13‐methylheptacosane and 11‐methylnonacosane were more abundant in females at the beginning and ending of the reproductive periods. Compound 11/13‐methylheptacosane was also more representative in female prothorax than in males, and 2‐methyloctacosane was richer in male elytra than in females.
We also studied the role of cuticular hydrocarbons in mate recognition in arena bioassays. Treatment of solvent‐washed dead females and glass dummies with one female equivalent (FE) of cuticular extract elicited mating responses in males, especially at the beginning of the reproductive period, with copulation attempts reaching 61.9% on solvent‐washed dead females and 23.8% on dummies. The successive treatment with synthetic compounds approaching a male cuticular profile inhibited male response.
Our results confirm that contact pheromones mediate mate recognition in C. welensii. Knowledge of the precise role played by the major compounds 11/13‐methylheptacosane and 11‐methylnonacosane and other minor compounds representative in female prothorax may contribute to the development of novel management strategies against C. welensii.
Family Feud: Succession Tournaments and Risk‐Taking in Family Firms*
Authors: Lee Jongsub , Shin Hojong , Yun Hayong 
Published: June 2023

AbstractWe identify succession as a novel determinant of risk‐taking in family firms. We find significantly higher risk‐taking (mergers and acquisitions and cash flow volatility) and lower operating efficiency in firms controlled by families with multiple sons during the pre‐ rather than the postsuccession period compared to family firms with one or no sons. Presuccession risk‐taking by sons decreases the following inheritance law amendments that require sharing of wealth among heirs, bolstering the causal interpretation of our findings. An infusion of outside talent via daughters' marriages also alleviates the relative rank‐seeking behaviors of sons during succession tournaments.
The Indexification of Poverty: The Covert Politics of Small‐Area Indices
Authors: Kiely Ed , Strong Samuel 
Published: June 2023

AbstractIn recent decades statistical indices have become a dominant method for measuring many features of the social world. While the resulting enumerations are regularly cited by critical human geographers, the wider political stakes of indexing the world remain unaddressed. In this article, we theorise indexification as the process through which composite statistics transform theoretical constructs into epistemic objects, and then geographically bounded rankings. Rather than a neutral process, we argue that these epistemological manoeuvres can mask various forms of violence. Through a detailed analysis of the UK's Index of Multiple Deprivation (IMD), we highlight the clandestine politics of indexification and their tendency to conceal harms meted out by the state. Seeking a more critical reckoning with indices, we conclude by calling for and outlining a project of radical indexification—a participatory, democratic, and transparent endeavour that takes spatial justice as its organising principle.
Distinct role of CD8 cells and CD4 cells in antitumor immunity triggered by cell apoptosis using a Herpes simplex virus thymidine kinase/ganciclovir system
Authors: Umegaki Sho , Shirota Hidekazu , Kasahara Yuki , Iwasaki Tomoyuki , Ishioka Chikashi 
Published: June 2023

Abstract
Immune cells can recognize tumor‐associated antigens released from dead tumor cells, which elicit immune responses, potentially resulting in tumor regression. Tumor cell death induced by chemotherapy has also been reported to activate immunity. However, various studies have reported drug‐induced immunosuppression or suppression of inflammation by apoptotic cells. Thus, this study aimed to investigate whether apoptotic tumor cells trigger antitumor immunity independent of anticancer treatment. Local immune responses were evaluated after direct induction of tumor cell apoptosis using a Herpes simplex virus thymidine kinase/ganciclovir (HSV‐tk/GCV) system. The inflammatory response was significantly altered at the tumor site after apoptosis induction. The expression of cytokines and molecules that activate and suppress inflammation simultaneously increased. The HSV‐tk/GCV‐induced tumor cell apoptosis resulted in tumor growth suppression and promoted T lymphocyte infiltration into tumors. Therefore, the role of T cells after inducing tumor cell death was explored. CD8 T cell depletion abrogated the antitumor efficacy of apoptosis induction, indicating that tumor regression was mainly dependent on CD8 T cells. Furthermore, CD4 T cell depletion inhibited tumor growth, suggesting the potential role of CD4 T cells in suppressive tumor immunity. Tumor tissues were evaluated after tumor cell apoptosis and CD4 T cell depletion to elucidate this immunological mechanism. Foxp3 and CTLA4, regulatory T‐cell markers, decreased. Furthermore, arginase 1, an immune‐suppressive mediator induced by myeloid cells, was significantly downregulated. These findings indicate that tumors accelerate CD8 T cell‐dependent antitumor immunity and CD4 T cell‐mediated suppressive immunity. These findings could be a therapeutic target for immunotherapy in combination with cytotoxic chemotherapy.
Development of teaching in ni‐Vanuatu children
Authors: Brandl Eva , Emmott Emily H. , Mace Ruth 
Published: June 2023

AbstractTeaching is an important mechanism of social learning. In industrialized societies, 3‐year‐olds tend to teach through demonstrations and short commands, while 5‐year‐olds use more verbal communication and abstract explanations. However, it remains unclear whether this generalizes to other cultures. This study presents results from a peer teaching game with 55 Melanesian children (4.7–11.4 years, 24 female) conducted in Vanuatu in 2019. Up to age 8, most participants taught through a participatory approach, emphasizing learning‐by‐doing, demonstrations, and short commands (57.1% of children aged 4–6 and 57.9% of children aged 7–8). Contrary to Western findings, abstract verbal communication only became common in children aged 9–11 (63.6%), suggesting that the ontogeny of teaching is shaped by the socio‐cultural environment.