bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of work-related musculoskeletal disorders and associated factors among University of Zimbabwe Faculty of Medicine and Health Sciences non-academic workers: a cross-sectional study
Authors: Tembo Letwin Nomalungelo , Munyikwa Jacquiline Paidamoyo , Musoro Chipo , Majonga Grace , Mavindidze Edwin 
Published: June 2023

Abstract
Background:Work-related musculoskeletal disorders most commonly contribute to years lived with disability among workers. Heavy physical work, static work posture, awkward posture, force exertion, lifting and repetitive movements increase risk of developing work-related musculoskeletal disorders.
Objectives: The aim of the study was to determine the prevalence of work-related musculoskeletal disorders and associated factors among non-academic workers at the University of Zimbabwe Faculty of Medicine and Health Sciences.
Methods and materials: The study used a cross-sectional analytical design.108 non-academic workers at University of Zimbabwe Faculty of Medicine and Health Sciences were sampled through stratified proportional convenient sampling. An adapted Nordic Musculoskeletal Questionnaire capturing socio-demographics and ergonomic risk factors was used to gather data. SPSS v24 was used for data analysis.
Results:100 non-academic workers from University of Zimbabwe Faculty of Medicine and Health Sciencesresponded. The 3-month and 12-month prevalence of work-related musculoskeletal disorders were highest in lower back (72% and 75%) and wrists/hands (60% and 69%) respectively. Sociodemographic factors such as age, gender, work experience, educational level and worker category were associated with work-related musculoskeletal disorders.
Discussion and conclusion: Prevalence of work-related musculoskeletal disorders was high among the participants. This was influenced by the nature of work as well as cultural factors.
Recommendations: Employers should consider developing wellness programs for the workers and promoting healthy working environments.
Fibulin-3 is necessary to prevent cardiac rupture following myocardial infarction
Authors: Murtha Lucy A. , Hardy Sean A. , Mabotuwana Nishani S. , Bigland Mark J. , Bailey Taleah , Raguram Kalyan , Liu Saifei , Ngo Doan T. , Sverdlov Aaron L. , Tomin Tamara , Birner-Gruenberger Ruth , Hume Robert D. , Iismaa Siiri E. , Humphreys David T. , Patrick Ralph , Chong James J.H. , Lee Randall J. , Harvey Richard P. , Graham Robert M. , Rainer Peter P. , Boyle Andrew J. 
Published: June 2023

Abstract
Despite the high prevalence of heart failure in the western world, there are few effective treatments. Fibulin-3 is a protein involved in extracellular matrix (ECM) structural integrity, however its role in the heart is unknown. We have demonstrated, using single cell RNA-seq, that fibulin-3 was highly expressed in quiescent murine cardiac fibroblasts, with expression highest prior to injury and late post-infarct (from ~day-28 to week-8). In humans, fibulin-3 was upregulated in left ventricular tissue and plasma of heart failure patients. Fibulin-3 knockout (Efemp1-/-) and wildtype mice were subjected to experimental myocardial infarction. Fibulin-3 deletion resulted in significantly higher rate of cardiac rupture days 3-6 post-infarct, indicating a weak and poorly formed scar, with severe ventricular remodelling in surviving mice at day-28 post-infarct. Fibulin-3 knockout mice demonstrated less collagen deposition at day-3 post-infarct, with abnormal collagen fibre-alignment. RNA-seq on day-3 infarct tissue revealed upregulation of ECM degradation and inflammatory genes, but downregulation of ECM assembly/structure/organisation genes in Fibulin-3 knockout mice. GSEA pathway analysis showed enrichment of inflammatory pathways and a depletion of ECM organization pathways. Fibulin-3 originates from cardiac fibroblasts, is upregulated in human heart failure, and is necessary for correct ECM organisation/structural integrity of fibrotic tissue to prevent cardiac rupture post-infarct.
Functionalized Cellulose with EDTA-like Chelating Groups for Removal of Heavy Metals
Authors: Yang Lu , You Nan , Ding Baohong 
Published: June 2023

AbstractCellulose is a renewable and promising material. However, native cellulose has to face the challenge of the removal of heavy metals with low efficiency which limits its application. In this work, a cellulose derivative with EDTA-like chelating groups is designed and prepared by the chemical grafting of cellulose. Cellulose is partially oxidized to dialdehyde cellulose which is treated with 20% excess of diethylenetriamine through a Schiff base reaction for the preparation of the aminated cellulose. The amine groups of the aminated cellulose are carboxymethylated by reacting with 20% excess of bromoacetic acid through a substitution reaction. A EDTA-like cellulose derivative functionalized with multidentate N,O‑donor atoms (EDTA-CL) is obtained for accessing the adsorptive property of Cd2+and Pb2+. The high-efficient adsorption of the both ions by the EDTA-CL with high adsorptive amounts (Pb2+: 438.3 mg g− 1and Cd2+: 287.2 mg g− 1) can be accomplished by controlled parameters (pH in the range of 4–6 with contact time 30 min) using the dosage of 1 g L− 1of the EDTA-CL in aqueous solution. The adsorptive processes of the both ions onto the EDTA-CL can be well fitted by pseudo-second-order and Langmuir equations. Thermodynamics data reveal that the adsorption of the both ions onto the EDTA-CL is a spontaneous and endothermic process. The loaded EDTA-CL by simple acid-base treatment can be regenerated 5 times with loss of adsorptive amounts (Cd2+: 14% and Pb2+: 17%).
Visualization of Latent Fingerprints Based on Composites of Dye-Doped Cellulose with Red Emissive Fluorescence
Authors: Li Zhan-Chao , Li Da-Wu , Liu Zhao-Hui , Wu Li-Ping , lv Xiao-Sen 
Published: June 2023

Abstract
Novel cellulose-based composites by integrating the rhodamine B (RhB) with cellulose microparticles are developed and used to detect latent fingerprints (LFPs). The cellulose-based composites containing the various amounts of RhB (5.4 ± 0.2, 9.7 ± 0.3, and 19.6 ± 0.3 mg g− 1) with the good photostability are obtained by simple adsorption method. Under 365 nm light irradiation, cellulose-based composite can produce the bright red fluorescence in solid-state. The images of the LFPs on the surface of the substrates with variable textures and colors are detected with integral ridge patterns and finely clear detail characteristics in level 2 and 3. The fresh and aging LFPs can be finely detected by the cellulose-based composite with the little interference of background because the red-emissive can efficiently avoid the interference from self-fluorescence of the substrates. These results suggest that the cellulose-based composite with favorable applicability and dependability can be a promising candidate for the visualization of the LFPs.
First report of the Olive flathead gudgeon, Butis gymnopomus (Bleeker 1853) from Kerala waters with taxonomic notes on B. butis (Hamilton 1822) and B. koilomatodon (Bleeker 1849)
Authors: Hariprakash Krishnaprasad Puthupillayalukkal Kallarikkal , Varada Mugtha Sukumaran , Krishnasadanam Melbin Lal , Hamsa Anvar Ali Palakkaparambil 
Published: June 2023

Abstract
This article provides information on the taxonomy and distribution of the poorly known gobioid genus Butisfrom a tropical Ramsar site, Vembanad-Kole in Kerala state, India. The results of integrative taxonomy confirm the presence of B. gymnopomus in Kerala. Identity of the other two species – B. butis and B. koilomatodon in the study area was also confirmed through morphology and mitochondrial Cytochrome C Oxidase 1 gene. Butis gymnopomus is distinguished from B. butis by the absence of scales on the interorbital region and the absence of axillary scales. The interorbital region is narrow in B. gymnopomus compared to B. butis. The overall K2P genetic distance between the species was found to be 14%. The study highlights the necessity of a detailed study on systematics of gobioids based on large regional sampling.
Aquaporins (AQPs) as marker in the Physiology of inflammation and its interaction studies with Garcinol
Authors: Kalita Anuradha , Das Manas 
Published: June 2023

Abstract

Aquaporins mainly AQP1, AQP3 and AQP4 have been known to be involved in the pathophysiology of inflammation based on earlier reports. Thisin vitrostudy using both RAW264.7 and THP-1 cells aimed to evaluate the involvement of Aquaporins as potential target of inflammation and the efficacy of methanolic extract ofGarcinia(GME) and its potent phytocompound (Garcinol) against the different markers of inflammation including the Aquaporins involved. siRNA silencing of AQP3 was carried out in RAW264.7 cells followed by LPS stimulation (1µg/ml) and assessment of important markers of inflammation including NO, PGE2, TNF-α, IL-6, IL-1β, CCL20, iNOS and COX-2. To assess the anti-inflammatory potential ofGarciniaextract and its active component Garcinol, cells were treated with 1µg/ml LPS in the absence and presence of increasing concentrations of GME and Garcinol. During the experimental period, extract concentrations (115µg/ml and 230 µg/ml for RAW264.7; 118 µg/ml and 236 µg/ml for THP-1) and Garcinol concentrations (6µM and 12µM for RAW264; 3µM and 6µM for THP-1) based on the IC50determined by MTT assay were used. The anti-inflammatory effects were assessed by measuring the levels of secretory proteins of TNF-α, IL-1β, IL-6, and CCL20 in LPS-activated cells. qPCR and Western blot analysis were performed to measure the AQP expression both at transcriptional and translational levels respectively. AQP3 knock down significantly decreased the NO, PGE2, TNF-α, IL-1β levels as well as iNOS and COX-2 mRNA expression. LPS stimulation led to significant increase in the mRNA and protein level expression AQP1, AQP3 and AQP4 in RAW264.7 cells; AQP1 and AQP3 in THP-1 cells indicating their role as markers of inflammation. GME and Garcinol effectively suppressed the LPS-induced proinflammatory cytokine release in dose-dependent manner in both the cell lines. These results indicate that AQP1, AQP3 and AQP4 could play a crucial role as markers of inflammation. Anti-inflammatory agents likeGarciniacould potentially decrease the expression of such AQPs, thus inhibiting the process of inflammation.
Morbidity and Etiology-Based Success Rate of Combined Endoscopic Ventriculostomy and Choroid Plexus Cauterization: A Systematic Review and Meta-analysis of 1918 Infants
Authors: Albalkhi Ibrahem , Garatli Sarah , Helal Baraa , Saleh Tariq , Ramadan Abdullah Al , Warf Benjamin Curtis 
Published: June 2023

Abstract
Approaches to the treatment of infant hydrocephalous vary among centers. Standard shunting carries a significant infection rate, an unpredictable time-to-failure, and the life-long risk of recurrent failures. Combined choroid plexus cauterization (CPC) and endoscopic third ventriculostomy (ETV) has been increasingly employed over the past decade as an alternative approach in an attempt to avoid shunt dependency. We performed a systematic review and meta-analysis to explore the reported morbidity associated with ETV/CPC and its rate of success reported for specific etiologies of infant hydrocephalus. The protocol of this study was registered with the International prospective register of Systematic Reviews (PROSPERO) with the following registration number CRD 42022343898. The study utilized four databases of medical literature to perform a systematic search following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Several parameters were extracted from the included studies including authors, publication year, region, study design, sample size, mean age, success rate, complication rate, reported complications hydrocephalus etiology, median time-to-failure, secondary management after failure and mean follow up time. The outcomes of interest, success, and complication rates were pooled using 95% confidence intervals (CI) and a random effects model. Heterogeneity was assessed using the I2 test. Twenty-eight studies met the inclusion criteria from an initial search result of 472 studies. The study included 1938 infants (1918 of which were included in the meta-analysis). The overall success rate of combined ETV/CPC is 0.59 (95% CI [0.53, 0.64], I2 = 82%). Etiology-based success rate is 0.71, 0.70, 0.64, and 0.52 for aqueductal stenosis, myelomeningocele, postinfectious hydrocephalus, and posthemorrhagic hydrocephalus, respectively. The overall complication rate is 0.04 (95% CI [0.02, 0.05], I2 = 14%). Our study presents a comprehensive analysis of the current evidence on the use of ETV/CPC for treating hydrocephalus in infants. The findings demonstrate the potential efficacy of this procedure; however, it is crucial to acknowledge the limitations inherent in the included studies, such as selection bias and limited follow-up, which could have impacted the reported outcomes.
Stimulus awareness is associated with secondary somatosensory cortex activation in an inattentional numbness paradigm
Authors: Peters Antje , Bruchmann Maximilian , Dellert Torge , Moeck Robert , Schlossmacher Insa , Straube Thomas 
Published: June 2023

Abstract
While inattentional blindness and deafness studies have revealed neural correlates of consciousness (NCC) without the confound of task relevance in the visual and auditory modality, comparable studies for the somatosensory modality are lacking. Here, we investigated NCC using functional magnetic resonance imaging (fMRI) in an inattentional numbness paradigm. Participants (N = 44) received weak electrical stimulation on the left hand while solving a demanding visual task. Half of the participants were informed that task-irrelevant weak tactile stimuli above the detection threshold would be applied during the experiment, while the other half expected stimuli below the detection threshold. Unexpected awareness assessments after the experiment revealed that altogether 10 participants did not consciously perceive the somatosensory stimuli during the visual task. Awareness was not significantly modulated by prior information. The fMRI data show that awareness of stimuli led to increased activation in the contralateral secondary somatosensory cortex. We found no significant effects of stimulus awareness in the primary somatosensory cortex or frontoparietal areas. Thus, our results support the hypothesis that somatosensory stimulus awareness is mainly based on activation in higher areas of the somatosensory cortex and does not require strong activation in extended anterior or posterior networks, which is usually seen when perceived stimuli are task-relevant.
Antidepressant use during pregnancy and the risk of preterm birth – a cohort study
Authors: Amit Guy , Yanover Chen , Bivas-Benita Maytal , Girshovitz Irena , Helbitz Tal , Sudry Tamar , Bar Vered , Tang Alice , Le Brian , Roger Jacquelyn , Woldemariam Sarah , Costello Jean , Kosti Idit , Oskotsky Tomiko T. , Akiva Pinchas , Sirota Marina 
Published: June 2023

Abstract
Background
Major depressive disorder and exposure to antidepressants during pregnancy have been previously associated with preterm birth (PTB). However, the reported results are difficult to interpret due to the inherent confounding by indication bias. We aimed to estimate the disentangled effects of antidepressants and maternal depression on the risk of PTB using data from electronic health records (EHRs).
Methods
This is a population-based retrospective cohort study, utilizing data from a primary care EHR. The cohort included 216,070 deliveries of 176,866 patients from the United Kingdom between January 1996 and February 2019. We analyzed the effects of antidepressant exposure, in particular selective serotonin reuptake inhibitors (SSRI), during pregnancy on the risk of PTB and additional control outcomes in subgroups of patients with a history of depression, and with recent pre-pregnancy treatment. We also assessed the effect of depression alone, in a subgroup of untreated patients. The analysis utilized large-scale propensity score matching that included all demographic and clinical covariates (conditions, drug prescriptions, observations, and procedures), and effect estimation using a Cox model.
Results
The main cohort included 17,615 (8.2%) PTBs of 16,946 mothers. Among patients who were not exposed to antidepressants during pregnancy, a history of depression was associated with an increased risk of PTB (HR 1.10, CI [1.04,1.15], P < 0.01]. Patients exposed to SSRIs during the first 22 weeks of gestation were at a marginally higher risk of PTB (during weeks 23 to 37) compared to unexposed patients (HR 1.08, CI [0.98,1.18], P = 0.11). However, subgroup analysis demonstrated that among patients with pre-pregnancy use of SSRIs, treatment continuation during pregnancy did not affect the risk of PTB (HR 1.03 [0.92,1.15], P = 0.61), as well as among patients with history of depression (HR 1.00 [0.90,1.11], P = 0.98). Additionally, in these subgroups exposure to antidepressants was associated with a reduced risk of some common medical conditions during the PTB follow-up window. These conditions, which are otherwise increased by depression, included upper respiratory infection, constipation, and headache.
Conclusion
Depression is associated with a higher risk of PTB, while exposure to antidepressants during pregnancy does not affect the observed risk. The clinical decision regarding antidepressant treatment during pregnancy should therefore consider the patient-specific risk-benefit potential, regardless of the concern of PTB.
A composite of blood-based biomarkers to distinguish major depressive disorder and bipolar disorder in adolescents and adults
Authors: Huang Jieping , Hou Xuejiao , Li Moyan , Xue Yingshuang , An Jiangfei , Wen Shenglin , Wang Zi , Cheng Minfeng , Yue Jihui 
Published: June 2023

Abstract
Objective
The purpose of the present study was to construct a diagnostic model to distinguish major depressive disorder (MDD) and bipolar disorder (BD) using potential commonly tested blood biomarkers.
Methods
Information of 721 inpatients with an ICD-10 diagnosis of major depressive disorder or bipolar disorder were collected from the electronic medical record system. Variables in the nomogram were selected by best subset selection method after a prior univariable screening, and then constructed using logistic regression with inclusion of the psychotropic medication use. The discrimination, calibration and internal validation of the nomogram were evaluated by the receiver operating characteristic curve, the calibration curve, cross validation and subset validation method.
Results
The nomogram consisted of five variables, including age, eosinophil count, plasma concentrations of prolactin, total cholesterol, and low-density lipoprotein cholesterol. The model could discriminate between MDD and BD with an AUC of 0.858, with a sensitivity of 0.716 and a specificity of 0.890.
Conclusion
The comprehensive nomogram constructed by the present study can be convenient to distinguish MDD and BD since the incorporating variables were common indicators in clinical practice. It could help avoid misdiagnoses and improve prognosis of the patients.
Carpets with visual cues can improve gait in Parkinson’s disease patients: may be independent of executive function
Authors: Hu Ze-Di , Zhu Shi-Guo , Huang Jie-Fan , Chen Jin-Yu , Huang Shi-Shi , Liu Rong-Pei , Chen Zhu-Ling , Ma Lu-Lu , Zhang Xiong , Wang Jian-Yong 
Published: June 2023

Abstract
Background Gait impairment is common in Parkinson’s disease (PD) patients, which greatly reduces their quality of life. Executive dysfunction is associated with gait impairment. Compensatory strategies, including visual cues, have been shown to be effective in improving PD gait. In this study, we aimed to understand whether carpets with visual cues could improve PD gait, and how the improvement varies across patients with different executive function state.Methods We designed carpets with chessboard and stripe cues. A total of 65 Chinese PD patients were recruited. Movement Disorder Society Unified Parkinson’s Disease Rating Scale, L-dopa equivalent daily dosage, Hoehn & Yahr stage, Frontal Assessment Battery, Mini Mental State Examination Scale, Hamilton Anxiety Scale, and Hamilton Depression Scale were evaluated. Gait parameters including stride length, gait speed and fall risk were recorded by a wearable electronic device.Results The stride length and gait speed were significantly improved and the fall risk was significantly mitigated when PD patients walked on carpets with chessboard and stripe patterns. Further analysis showed the amelioration of gait parameters was independent of executive dysfunction.Conclusions Our study demonstrates that carpets with visual cues can improve the gait of PD patients even in those with partial executive dysfunction.
The impact of chronic TMAO administration on liver oxidative stress, inflammation, and fibrosis
Authors: Florea Cristian Marius , Rosu Radu , Moldovan Remus , Vlase Laurian , Toma Vlad , Decea Nicoleta , Baldea Ioana , Filip Gabriela Adriana 
Published: June 2023

Abstract
Purpose
TMAO, a gut microbiota derived byproduct, has been associated with various cardiometabolic diseases by promoting oxidative stress and inflammation. The liver is the main organ for TMAO production and chronic exposure to high doses of TMAO could alter its function. In the present study we evaluated the effect of chronic exposure of high TMAO doses on liver oxidative stress, inflammation and fibrosis.
Methods
TMAO was administered daily via gastric gavage to 24 male Wistar rats for 3 months. The animals were divided in three groups, a high-dosage group that received 40 mg of TMAO/day, a low-dose group that received 20 mg of TMAO/day and a control group treated with vehicle. Blood was drawn for the quantification of TMAO and liver tissues were harvested for the assessment of oxidative stress (MDA, GSH, GSSG, GPx, CAT, and 8-oxo-dG) and inflammation by quantification of NOS and COX-2 expression. The evaluation of fibrosis was made by western blot analysis of α-SMA and Collagen-3 protein expression. Moreover, histological investigation and immunohistochemical staining of iNOS were performed in order to assess the liver damage.
Results
After 3 months of TMAO exposure, TMAO serum levels enhanced in parallel with significant increases in MDA and GSSG levels in liver and lower values of GSH and GSH/GSSG ratio as well as significant decrease in GPx and CAT activities. Inflammation was also highlighted, with a significant increase in iNOS and COX-2 expression, but without structural changes and without induction of liver fibrosis.
Conclusion
Chronic administration of TMAO for 3 months induced liver oxidative stress and inflammation without the appearance of fibrosis or liver structural changes.