bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zuogui Jiangtang Shuxin formula Ameliorates diabetic cardiomyopathy mice via modulating gut-heart axis
Authors: Huang Ya-lan , Xiang Qin , Zou Jun-ju , Wu Yongjun , Yu Rong 
Published: February 2023

BackgroundThere is growing evidence demonstrating that the gut microbiota plays a crucial role in multiple endocrine disorders, including diabetic cardiomyopathy (DCM). Research shows that the Chinese herb reduces disease occurrence by regulating gut microbiota. Zuogui Jiangtang Shuxin formula (ZGJTSXF), a Chinese medicinal formula, has been clinically used for treatment of DCM for many years. However, there is still no clear understanding of how ZGJTSXF treatment contributes to the prevention and treatment of DCM through its interaction with gut microbiota and metabolism.MethodsIn this study, mice models of DCM were established, and ZGJTSXF’s therapeutic effects were assessed. Specifically, serum glycolipid, echocardiography, histological staining, myocardial apoptosis rate were assessed. Using 16s rRNA sequencing and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), we determined the impact of ZGJTSXF on the structure of gut microbiota and content of its metabolite TMAO. The mechanism of ZGJTSXF action on DCM was analyzed using quantitative real-time PCR and western blots.ResultsWe found that ZGJTSXF significantly ameliorated DCM mice by modulating gut-heart axis: ZGJTSXF administration improved glycolipid levels, heart function, cardiac morphological changes, inhibited cardiomyocytes apoptosis, and regulate the gut microbiota in DCM mice. Specifically, ZGJTSXF treatment reverse the significant changes in the abundance of certain genera closely related to DCM phenotype, including Lactobacillus, Alloprevotella and Alistipes. Furthermore, ZGJTSXF alleviated DCM in mice by blunting TMAO/PERK/FoxO1 signaling pathway genes and proteins.ConclusionZGJTSXF administration could ameliorate DCM mice by remodeling gut microbiota structure, reducing serum TMAO generation and suppressing TMAO/PERK/FoxO1 signaling pathway.
Assessment of oligometastasis status of prostate cancer following combined robot-assisted radical prostatectomy and androgen deprivation versus androgen deprivation therapy alone using PSA percentage decline rate
Authors: Li Xuwen , Xi Haibo , Cheng Xiaofeng , Yu Yue , Zhang Cheng , Wang Gongxian , Zhou Xiaochen 
Published: February 2023

ObjectiveTo compare the tumor control in prostate cancer patients with oligo-metastasis following combined robot-assisted radical prostatectomy and androgen deprivation versus androgen deprivation therapy alone based on total prostate-specific antigen (tPSA) assessment.MethodsMedical data of a total of 18 prostate cancer patients with oligometastasis administered in The First Affiliated Hospital of Nanchang University from March 2017 to March 2018 were prospectively collected. 10 patients received a combined therapy of robot-assisted radical prostatectomy and pharmaceutical androgen deprivation (RARP+ADT group), while 8 patients received pharmaceutical androgen deprivation therapy alone (ADT group). Then demographic characteristics, prostate volume, tumor characteristics and tPSA data were analysised and compared. Statistical analysis was performed using t-test for continuous variables and Pearson chi-square test or Fisher’s exact test for categorical variables.ResultsNo significant difference was found in patients’ age (p = 0.075), prostate volume (p = 0.134) and number of bone metastasis (p = 0.342). Pre-treatment Gleason score was significantly lower in RA group (p = 0.003). Patients in RARP+ADT group had significantly lower pre-treatment tPSA (p = 0.014), while no statistical difference was noted in reexamined tPSA (p = 0.140) on follow-up. No statistical difference was noted in tPSA decline rates (declined tPSA value per day) in RARP+ADT and ADT group (8.1 ± 4.7 verse 7.5 ± 8.0 ng/ml/d, p = 0.853). However, tPSA percentage decline rate (declined tPSA percentage per day) was significantly higher in RARP+ADT group (11.6 ± 1.5%/d verses 2.9 ± 2.2%/d, p&lt; 0.001). Immediate urinary continence was achieved in 9 patients (90%) upon removal of urethral catheter on post-operative day 7 in RARP+ADT group.ConclusionADT alone and in combination with RARP both provide effective tumor control in patients suffering from prostate cancer with oligometastasis. ADT combined with RARP exhibited significant advantage in PSA percentage decline rate without compromising patients’ urinary continence. Long-term tumor control requires further follow-up.
Variations, sources, and effects on ozone formation of VOCs during ozone episodes in 13 cities in China
Authors: Shan Danying , Du Zhenyu , Zhang Ting , Zhang Xiulan , Cao Guan , Liu Zirui , Yao Zhixiao , Tang Ka , Liang Siyuan 
Published: February 2023

In recent years, ozone (O3) pollution has worsened in China and contributes frequently to air pollution problems. To support the implementation of coordinated control for ozone and fine particulate matter, it is essential to study the chemical compositions and sources of volatile organic compounds (VOCs), which are the crucial precursor of both ozone and fine particulate matter. In this study, 117 volatile organic compounds were monitored in 13 cities in Beijing-Tianjin-Hebei Urban Agglomeration and Fenwei plain. Concentrations of total volatile organic compounds ranged from 42 to 279 μg/m3 during the monitoring episode. In all 13 cities, alkanes, halogenated hydrocarbons, aromatics and oxygenated volatile organic compounds (OVOCs) were the dominant volatile organic compounds. Contributions of alkanes, halogenated hydrocarbons, aromatics and oxygenated volatile organic compounds to total ozone formation potential (OFP) were 21.7%–32.6%, 21.0%–27.9%, 24.3%–50.8% and 28.6%–52.3%, respectively. Furthermore, the results of source apportionment by positive matrix factorization (PMF) model indicated that solvent usage, gasoline evaporation, vehicle emissions, petrochemical industry and combustion were essential volatile organic compounds sources in 13 cities. Moreover, the sensitivity of ozone production was studied using an Empirical Kinetic Modeling Approach (EKMA) model, and it was found that ozone formation was volatile organic compounds limited in all 13 cities.
Diversity of soil fertility management options in maize-based farming systems in northern Benin: A quantitative survey
Authors: Tovihoudji Pierre G. , Akpo Firmin I. , Tassou Zakari F. , Ollabodé Nouroudine , Yegbemey Rosaine N. , Yabi Jacob A. 
Published: February 2023

Introduction: Maize-based production systems in Sub-Saharan Africa are largely based on family farming, which is characterized by low-input, nutrient-mining agriculture and practices. These systems usually promote soil degradation through loss of organic matter and erosion. The present study characterizes farms and main soil fertility management options in maize-based farming systems of the northern Benin.Methods: The study was conducted in the municipalities of Malanville, Banikoara and Bembèrèkè. We sampled randomly and interviewed 262 maize farmers, and the statistical analysis (distribution of means and frequencies, chi-square, ANOVA, etc.) was performed.Results and discussion: The results show that maize farming characteristics and socioeconomic conditions (land use and labor, production activities and land allocation, institutional arrangements on land, access to labor and capital, etc.) were diverse across locations and exhibited a wide variation within locations. Several practices were used for the management and sustainable maintenance of soil fertility in maize production systems in northern Benin: maize-legume intercropping, cotton-maize rotation, and mineral and organic fertilizers application. Most of farmers occasionally or regularly used mineral fertilizers (95.4%), followed by legume-cereal rotation/ intercropping (51.9%). Overall, 23.6% and 58.4% of farmers consistently used mineral fertilizer over the last 5 and 10 years, respectively. The amount of applied mineral fertilizer did not significantly vary between locations with an average (applied day after sowing, DAS) of 131.7 ± 13.7 (22 ± 8 DAS), 58.7 ± 9.6 (44 ± 5 DAS) and 164.7 ± 25.4 (38 ± 11 DAS) kg ha−1 for NPK, urea and Mix NPK + urea, respectively applied at, and days after sowing. Most farmers spread the fertilizer around the plants without covering with soil particles. Manure was applied exclusively to food crops through transporting and corralling (28.2%); and most farmers also used manure from their own livestock while few farmers used cattle corralling. Farmyard manure was mainly spread (100% of respondents) on the surface before plowing at the beginning of the rainy season. Maize farmers applied mineral fertilizer based on the level of initial soil fertility (naturally fertile or poor, degree of erosion etc.) and fertilizer purchase costs. Manure was not widely used as an alternative to chemical fertilizers; therefore, farmers need more strengthening and technical assistance on the production of organic fertilizers and manure storage. The findings are useful for policymakers on encouraging the successful implementation of sustainable soil fertility management strategies of maize-based farming systems in northern Benin.
Analytical validation and implementation of a pan cancer next-generation sequencing panel, CANSeqTMKids for molecular profiling of childhood malignancies
Authors: Schilter Kala F. , Smith Brandon A. , Nie Qian , Stoll Kathryn , Felix Juan C. , Jarzembowski Jason A. , Reddi Honey V. 
Published: February 2023

Next-Generation Sequencing (NGS) allows rapid analysis of multiple genes for the detection of clinically actionable variants. This study reports the analytical validation of a targeted pan cancer NGS panel CANSeqTMKids for molecular profiling of childhood malignancies. Analytical validation included DNA and RNA extracted from de-identified clinical specimens including formalin fixed paraffin embedded (FFPE) tissue, bone marrow and whole blood as well as commercially available reference materials. The DNA component of the panel evaluates 130 genes for the detection of single nucleotide variants (SNVs), Insertion and Deletions (INDELs), and 91 genes for fusion variants associated with childhood malignancies. Conditions were optimized to use as low as 20% neoplastic content with 5 ng of nucleic acid input. Evaluation of the data determined greater than 99% accuracy, sensitivity, repeatability, and reproducibility. The limit of detection was established to be 5% allele fraction for SNVs and INDELs, 5 copies for gene amplifications and 1,100 reads for gene fusions. Assay efficiency was improved by automation of library preparation. In conclusion, the CANSeqTMKids allows for the comprehensive molecular profiling of childhood malignancies from different specimen sources with high quality and fast turnaround time.
Prognostic roles of a novel basement membranes-related gene signature in lung adenocarcinoma
Authors: Zhu Xingzhuang , Liu Xiaoyan , Qiu Xiaowen , Niu Zihao , Dong Wei , Song Yipeng 
Published: February 2023

Background: The basement membranes (BMs) are involved in tumor progression, while few comprehensive analyses to date are performed on the role of BM-related gene signatures in lung adenocarcinoma (LUAD). Thus, we aimed to develop a novel prognostic model in LUAD based on BMs-related gene profiling.Methods: The LUAD BMs-related gene profiling and corresponding clinicopathological data were obtained from the basement membrane BASE, The Cancer Genome Atlas (TCGA) and gene expression omnibus (GEO) databases. The Cox regression and least absolute shrinkage and selection operator (LASSO) methods were used to construct a BMs-based risk signature. The concordance index (C-index), receiver operating characteristic (ROC), and calibration curves were generated to evaluate the nomogram. The GSE72094 dataset was used to validate prediction of the signature. The differences in functional enrichment, immune infiltration, and drug sensitivity analyses were compared based on risk score.Results: In TCGA training cohort, 10 BMs-related genes were found, (e.g., ACAN, ADAMTS15, ADAMTS8, BCAN, etc). The signal signature based on these 10 genes was categorized into high- and low-risk groups regarding survival differences (p &lt; 0.001). Multivariable analysis showed that the signature of combined 10 BMs-related genes was an independent prognostic predictor. Such a prognostic value of BMs-based signature in validation cohort of the GSE72094 were further verified. The GEO verification, C-index, and ROC curve showed that the nomogram had accurate prediction performance. The functional analysis suggested that BMs were mainly enriched in extracellular matrix-receptor (ECM-receptor) interaction. Moreover, the BMs-based model was correlated with immune checkpoint.Conclusion: This study identified BMs-based risk signature genes and demonstrated their ability to predict prognosis and guide personalized treatment of patients with LUAD.
COVID-19 vaccine uptake, hesitancy and clinical effects on patients with Takayasu’s arteritis: A web-based questionnaire survey from a large cohort
Authors: Kong Xiufang , Dai Xiaojuan , Ma Lingying , Wang Jinghua , Sun Ying , Jiang Lindi 
Published: February 2023

ObjectiveThis study aimed to investigate the Coronavirus disease 2019 (COVID-19) vaccination rate, reasons for vaccine hesitancy and clinical effects on patients with Takayasu’s arteritis (TAK).MethodsA web-based survey was administered to a TAK cohort established by the Department of Rheumatology, Zhongshan Hospital through WeChat in April, 2022. Responses from a total of 302 patients were received. The Sinovac or Sinopharm inactivated vaccination rate, side effects, and vaccine hesitancy reasons were analyzed. In addition, disease flare, new disease onset, and changes of immune-related parameters after vaccination were analyzed in vaccinated patients.ResultsAmong 302 patients, 93 (30.79%) received the inactivated COVID-19 vaccination. Among the 209 unvaccinated patients, the most common reason for hesitancy were concern about side effects (136, 65.07%). Vaccinated patients had a longer disease duration (p = 0.08) and lower use of biologic agents (p &lt; 0.001); 16 (17.20%) of the 93 vaccinated patients developed side effects, and most of them were mild; 8 (8.60%) developed disease flares or new-onset disease 12-128 days post-vaccination and 2 (2.15%) developed serious adverse effects (vision defect and cranial infarction). Immune-related parameters of 17 patients indicated decreases in IgA and IgM after vaccination (p &lt; 0.05). Eighteen (19.35%) of the 93 vaccinated patients were diagnosed post-vaccination.These patients had a significantly higher percentage of CD19+ B cells at disease onset (p &lt; 0.05) than the unvaccinated patients diagnosed at the same time.ConclusionThe vaccination rate was low in TAK, which was mainly caused by concerns about negative effects of vaccination on their disease. An acceptable safety profile was observed in vaccinated patients. The risk of disease flare associated with COVID-19 vaccination warrants further investigation.
Role of immune cell infiltration and small molecule drugs in adhesive capsulitis: Novel exploration based on bioinformatics analyses
Authors: Liu Hailong , Yu Baoxi , Deng Zengfa , Zhao Hang , Zeng Anyu , Li Ruiyun , Fu Ming 
Published: February 2023

BackgroundAdhesive capsulitis (AC) is a type of arthritis that causes shoulder joint pain, stiffness, and limited mobility. The pathogenesis of AC is still controversial. This study aims to explore the role of immune related factors in the occurrence and development of AC.MethodsThe AC dataset was downloaded from Gene Expression Omnibus (GEO) data repository. Differentially expressed immune-related genes (DEIRGs) were obtained based on R package “DESeq2” and Immport database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed to explore the functional correlation of DEIRGs. MCC method and Least Absolute Shrinkage and Selection Operator (LASSO) regression were conducted to identify the hub genes. The immune cell infiltration in shoulder joint capsule between AC and control was evaluated by CIBERSORTx, and the relationship between hub genes and infiltrating immune cells was analyzed by Spearman’s rank correlation. Finally, potential small molecule drugs for AC were screened by the Connectivity Map database (CMap) and further verified by molecular docking.ResultsA total of 137 DEIRGs and eight significantly different types of infiltrating immune cells (M0 macrophages, M1 macrophages, regulatory T cells, Tfh cells, monocytes, activated NK cells, memory resting CD4+T cells and resting dendritic cells) were screened between AC and control tissues. MMP9, FOS, SOCS3, and EGF were identified as potential targets for AC. MMP9 was negatively correlated with memory resting CD4+T cells and activated NK cells, but positively correlated with M0 macrophages. SOCS3 was positively correlated with M1 macrophages. FOS was positively correlated with M1 macrophages. EGF was positively correlated with monocytes. Additionally, dactolisib (ranked first) was identified as a potential small-molecule drug for the targeted therapy of AC.ConclusionsThis is the first study on immune cell infiltration analysis in AC, and these findings may provide a new idea for the diagnosis and treatment of AC.
Urinary exosome tsRNAs as novel markers for diagnosis and prediction of lupus nephritis
Authors: Chen Shanshan , Zhang Xiaoshan , Meng Kaifang , Sun Yifan , Shu Ruilu , Han Yan , Feng Qingxiu , Li Zhiyang , Yang Ping , Liang Jun 
Published: February 2023

ObjectiveLupus nephritis (LN) is one of the most severe organ manifestations of systemic lupus erythematosus (SLE). Early identification of renal disease in SLE is important. Renal biopsy is currently recognized as the gold standard for diagnosing LN, however, it is invasive and inconvenient for dynamic monitoring. Urine has been considered more promising and valuable than blood in identifying inflamed kidney tissue. Here, we determine whether the signatures of tRNA-derived small noncoding RNA (tsRNA) in urinary exosomes can serve as novel biomarkers for the diagnosis of LN.MethodstsRNA sequencing was performed in exosome extracted from pooled urine of 20 LN patients and 20 SLE without LN, and the top 10 upregulated tsRNAs were screened as candidate markers of LN. The candidate urinary exosomal tsRNAs were primarily elected by TaqMan probe-based quantitative reverse transcription-PCR (RT-PCR) in 40 samples (20 LN and 20 SLE without LN) in the training phase. In the validation phase, selected tsRNAs from the training phase were further confirmed in a larger cohort (54 LN patients and 39 SLE without LN). Receiver operating characteristic curve (ROC) analysis was conducted to evaluate the diagnostic efficacy.ResultsUpregulated levels of tRF3-Ile-AAT-1 and tiRNA5-Lys-CTT-1 in the urinary exosomes were observed in LN compared with SLE without LN (P &lt; 0.0001 and P &lt; 0.001) and healthy controls (P &lt; 0.01 and P &lt; 0.01), with the area under the curve (AUC) of 0.777 (95% CI: 0.681-0.874, sensitivity 79.63%, specificity 66.69%) and 0.715 (95% CI: 0.610-0.820, sensitivity 66.96%, specificity 76.92%) for discriminating LN from SLE without LN patients. SLE patients with mild activity and moderate to severe activity had higher levels of urinary exosome derived tRF3-Ile AAT-1 (P = 0.035 and P &lt; 0.001) and tiRNA5-Lys-CTT-1 (P = 0.021 and P &lt; 0.001) compared with patients with no activity. Moreover, bioinformatics analysis revealed that both of the tsRNAs regulate the immune process by modulating metabolism and signal pathway.ConclusionIn this study, we demonstrated that urinary exosome tsRNAs can be served as noninvasive biomarkers for the efficient diagnosis and prediction of nephritis in SLE.
Depth and temperature profiles reflect individual differences in the daytime diving behaviours of pelagic thresher sharks
Authors: Oliver Simon P. , Grothues Thomas M. , Mayo Zoe J. , Williams Amie L. , Silvosa Medel , Cases Gary 
Published: February 2023

We used acoustic telemetry to investigate the roles of depth and temperature in the daytime foraging behaviours of 13 tagged pelagic thresher sharks by monitoring their fine scale vertical movements in the Philippines. Cumulatively, pelagic thresher shark dives traversed the entire water column where they encountered temperatures that ranged from 33°C at the surface to 12°C at 250 m depths throughout the day, but the movements of individuals varied in the extent of both their deep and shallow water limits. Dives were not synchronized to diurnal cycles, and periodicity reflected cycles of similar dives, the dives themselves, deviations, cruising, and individuality. Pelagic thresher shark movements between the warm surface layer and cooler waters below the thermocline (155 – 175 m) may reflect a common Alopiid strategy that balances maintaining tolerable ambient water temperatures with opportunities to search for and forage on spatially patchy distributions of prey.
Impact of the COVID-19 pandemic on imaging case volumes in King Abdullah University Hospitals (KAUH)
Authors: Gharaibeh Maha , El-Obeid Eyhab , Khasawneh Ruba , Karrar Musaab , Salman Mohamed , Farah Ahmad , Ahmmed Sammah , Al-Omari Mamoon , Elheis Mwaffaq , Abualigah Laith 
Published: February 2023

ObjectiveCOVID-19 has an increased burden on the delivery of services because the measures taken by the governments forced hospitals to cancel most of their elective procedures and led to the shutting down of outpatient clinics. This study aimed to evaluate the impact COVID-19 pandemic on the volume of radiology exams based on patient service locations and imaging modality in the North of Jordan.MethodsThe imaging case volumes that were performed at the King Abdullah University Hospital (KAUH), Jordan, from 1 January 2020 to 8 May 2020, were retrospectively collected and compared to those from 1 January 2019 to 28 May 2019, to determine the impact of the pandemic of COVID-19 on the volume of radiological examinations. The 2020 study period was chosen to cover the peak of COVID-19 cases and to record the effects on imaging case volumes.ResultsA total of 46,194 imaging case volumes were performed at our tertiary center in 2020 compared to 65,441 imaging cases in 2019. Overall, the imaging case volume in 2020 decreased by 29.4% relative to the same period in 2019. The imaging case volumes decreased for all imaging modalities relative to 2019. The number of nuclear images showed the highest decline (41.0%) in 2020, followed by the number of ultrasounds (33.2%). Interventional radiology was the least affected imaging modality by this decline, with about a 22.9% decline.ConclusionThe number of imaging case volumes decreased significantly during the COVID-19 pandemic and its associated lockdown. The outpatient service location was the most affected by this decline. Effective strategies must be adopted to avoid the aforementioned effect on the healthcare system in future pandemics.