bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Remote Sensing Techniques in Determining the Risk Taking Level of Different Seasons on Fire Generation in Terms of NDVI Index During the Year Case Study: Golestan Province, Iran
Authors: Askari Kaveh Ostad Ali 
Published: January 2025

Abstract

Knowledge of the nature of seasons and months in terms of the fire risk is very important in environmental planning, land management and forest resource management in order to achieve the sustainable development. One of the applications of remote sensing in this regard is the continuous monitoring of the zone to detect changes. Currently, the vegetation mapping is used to generate information for macro and micro planning. In order to monitor changes across the Golestan province forests through different seasons in 2000-2015, all images of MOD13Q1 MODIS were prepared during this period. Then, the images of the Normalized Difference Vegetation Index (NDVI) were prepared for the four seasons and twelve months of the year. The classification of the indices included lands covered with excellent, moderate, weak and very poor coverage was conducted in order to investigate the changes. Then, the comparison was then performed by LAND FIRE points and the validity of the classification results was evaluated. It was concluded that the seasons of the year from high risk to low risk were winter, summer, fall and spring, respectively. In the high-risk season, winter, January was the most dangerous month and in the low risk season, spring, may was the lowest month of the year.
Cross-talk Between NLRP3 and AIM2 Inflammasomes in Macrophage Activation by LPS and Titanium Ions
Authors: Gálvez Ana Belén Carrillo 
Published: January 2025

Abstract

Background
Periodontitis and peri-implantitis are chronic inflammatory diseases that contribute to tissue destruction and bone loss. Periodontitis is triggered by pathogenic bacteria, while peri-implantitis also involves metallic particles, which increase the inflammatory response. Both conditions are linked to the activation of inflammasomes, such as NLRP3 and AIM2, which facilitate the release of pro-inflammatory cytokines like IL-1β and IL-18 and induce pyroptosis. This study aims to investigate the activation of NLRP3 and AIM2 inflammasomes in macrophages exposed to bacterial and metallic components, as well as to explore the potential interplay between these two signaling pathways.
Methods
Human THP-1-derived macrophages were treated with bacterial lipopolysaccharide (LPS) and titanium ions to evaluate inflammasome activation. IL-1β secretion, ROS production, mitochondrial DNA release and pyroptosis were assessed. Additionally, macrophages deficient in NLRP3 and AIM2 were used to examine the roles of these inflammasomes in inflammatory responses.
Results
LPS and titanium ions synergistically activated NLRP3, resulting in increased IL-1β secretion, ROS production, and pyroptosis. Under these conditions, AIM2 was indirectly activated, as indicated by elevated mitochondrial DNA release. Notably, AIM2 expression was reduced in wild-type macrophages treated with LPS and titanium ions compared to LPS alone, however, in NLRP3-deficient cells, AIM2 expression was increased following LPS and titanium ions treatment. This upregulation of AIM2 in NLRP3-deficient cells was further reduced by ROS inhibition, which decreased mitochondrial DNA release. Additionally, NLRP3 knockout had a more pronounced effect on reducing IL-1β secretion and pyroptosis compared to AIM2 knockout, indicating a greater role of NLRP3 in these inflammatory responses.
Conclusions
This study demonstrates that bacterial and metallic components drive the activation of both NLRP3 and AIM2 inflammasomes in macrophages, highlighting their roles in the inflammatory responses associated with periodontitis and peri-implantitis. The findings reveal a regulatory relationship between NLRP3 and AIM2, where the absence of one inflammasome can enhance the activity of the other. These results provide new insights into the mechanisms underlying inflammasome-mediated inflammation and suggest potential therapeutic targets for managing inflammatory diseases.
Prevalence and Associated Risk Factors of Typhoid Fever in Patients Attending Bishoftu town Health Centers, Oromia, Central Ethiopia
Authors: Mekonnen Gebeyehu , Tesfaye Solomon , Bekele Damtew , Asmare Esubalew 
Published: January 2025

Abstract

Typhoid fever remains a major public health problem globally and a major cause of morbidity in the developing world. The objective of the study was to assess the prevalence and associated risk factors of typhoid fever in Bishoftu town, central Ethiopia. A health center-based cross-sectional study was conducted in three health centers in Bishoftu Town, central Ethiopia from April to May 2022. Three hundred sixty-two blood samples were collected from patients attending in three health centers of Bishoftu town and were tested for typhoid by Widal test. Similarly, a pretested, structured questionnaire was used to collect socio-demographic and possible risk factors. SPSS version 22 was used for statistical analysis. Bivariate and multivariate logistic regression analyses were used to assess the association of various risk factors with typhoid prevalence. The overall prevalence of typhoid fever was 35.1%. Being female (AOR: 2.136, 95% CI: 1.012, 4.508, P = 0.047), not being able to read and write (AOR: 2.990, 95% CI: 1.903, 19.111, P = 0.007), not keeping food in hygienic conditions (AOR: 4.984, 95% CI: 1.574, 15.777, P = 0.006), open defecation (AOR: 3.914, 95% CI: 1.390, 10.660, P = 0.008), hand washing with soap before meals (AOR: 4.400, 95% CI: 1.390, 13.291, P = 0.012), hand washing with soap after defecations (AOR: 7.541, 95 CI: 2.309, 24.628, P = 0.001), and eating street food (AOR: 4.607, 95% CI: 0.778, 27.624, P = 0.001) were identified as associated risk factors to typhoid fever prevalence. The study showed typhoid fever is a significant public health problem in Bishoftu town and its surroundings. Therefore, care should be taken to keep food in hygienic conditions and to wash hands with soap before meals and after using the toilet in order to control the disease.
The Expression of the LDLR, LDLRAP1, and PCSK9 Genes has Prognostic Significance in Triple-negative Breast Cancer
Authors: Antipenko Ivan Denisovich , Olkhovik Darya Mikhailovna , Solopova Olga Nikolaevna , Khayretdinova Gulfia Amirovna , Kalacheva Olga Sergeevna , Makarova Julia Alekseevna , Shkurnikov Maxim Yurievich 
Published: January 2025

Aims:
The purpose of this study was to investigate the prognostic significance of
cholesterol uptake genes in predicting the survival of breast cancer patients.

Background:
Cholesterol plays a crucial role in the homeostasis of tumor cells. It is known
that cholesterol levels can influence important parameters of the disease, such as sensitivity
to therapy, progression, and metastasis of cancer. Previous studies suggest that breast cancer
subtypes exhibit differences in metabolism.

Objective:
The objectives of this study were to determine whether cholesterol uptake genes
have prognostic significance for overall survival in breast cancer patients, evaluate if this
prognostic significance varies between breast cancer subtypes, and identify differences in
the expression of cholesterol uptake genes among these subtypes.

Methods:
Data from mRNA sequencing of tumors from the Cancer Genome Atlas (TCGA)
portal were analyzed. Tumors were classified into molecular subtypes, and the prognostic
significance of cholesterol uptake gene expression levels was evaluated for each subtype.
DESeq2 and Fisher's test were used to assess differences in gene expression.

Results:
High expression levels of genes involved in de novo cholesterol synthesis were associated
with poor prognosis for the Basal-like and Luminal A breast cancer subtypes. The
prognostic significance of low-density lipoprotein receptor (LDLR), LDLR adapter protein
1 (LDLRAP1), and proprotein convertase subtilisin/kexin type 9 (PCSK9), which are responsible
for exogenous cholesterol uptake, varied across subtypes. Specifically, low expression
of LDLR was associated with a favorable prognosis for the luminal A (OR = 2.17;
FDR = 0.0048) and luminal B (OR = 2.21; FDR = 0.015) subtypes but indicated poor prognosis
in the basal-like subtype (OR = 0.48; FDR = 0.05). No genes were significant for prognosis
prediction in the HER2-positive subtype. The HER2+ subtype exhibited higher expression
of cholesterol uptake genes compared to the basal-like subtype based on the analysis of
tumor mRNA sequencing (OR = 6.45, p-value = 3.07E-05). This finding was also confirmed
through the study of publicly available single-cell sequencing data (OR = 40.3, p-value
= 2.19e-07), which may contribute to the differences in their prognostic significance.

Conclusion:
The prognostic significance of cholesterol uptake gene expression varies
among breast cancer subtypes. Precise fitting of biomarkers into breast cancer subtypes may
aid in more accurate patient stratification and improve treatment approaches.
The Effect of Gallic Acid on the Alleviation of the Chemotherapy-Induced Myelosuppression
Authors: Luo Junyi , Zhang Zhaoxia , Jin Liming , Wang Zhaoying , Sun Qiuyue , He Dawei 
Published: January 2025

Objective:
This study aims to investigate the effect of Gallic Acid (GA) on
the alleviation of chemotherapy-induced bone marrow suppression, with a comparison
to Diyu sheng bai tablets (DYSB) and RhG-CSF.

Methods:
A mouse model of bone marrow suppression was established in BALB/c mice
using intraperitoneal injections of cyclophosphamide (CTX). All procedures were performed
after obtaining ethical clearance from the institutional animal ethics committee.
Mice were treated with low (100 mg/kg/d), medium (200 mg/kg/d), and high (400
mg/kg/d) doses of Gallic Acid (GA) to mitigate CTX-induced bone marrow suppression.
In parallel, mice in the positive control group were also treated with DYSB and
RhG-CSF at their respective standard doses (DYSB: 100 mg/kg/day, RhG-CSF: 125
mg/kg/day). The efficacy of GA in alleviating chemotherapy-induced bone marrow suppression
was evaluated through blood cell counts, immune organ (thymus and spleen) indices,
bone marrow nucleated cell (BMNC) counts, cell cycle analysis, apoptosis, histopathology
of bone marrow and spleen, and analysis of splenic hematopoietic factors.

Results:
CTX induced a decrease in peripheral blood cells and BMNC counts, reduced
spleen and thymus indices, and diminished abnormal pathology of bone marrow and
spleen, as well as decreasing disturbances in hematopoietic factors. GA was able to alleviate
these abnormalities in the bone marrow. It modulated cell proliferation and apoptosis,
adjusted the proportion of cells in the G0/G1 phase, and reduced apoptosis in femoral
bone marrow.

Conclusion:
Gallic Acid (GA) alleviates chemotherapy-induced bone marrow suppression
by improving immune organ function, promoting bone marrow cell recovery, and
inhibiting apoptosis. These findings support GA as a potential adjunct therapy for chemotherapy,
with promising clinical applications.

conclusion:
GA can alleviate the atrophy of immune organs, relieve the proliferation blockade of bone marrow cells, inhibit bone marrow cell apoptosis, and promote the recovery of the spleen and hematopoietic factors, thereby mitigating CTX-induced bone marrow suppression. The study confirms the potential of the natural compound GA as an effective adjunct in alleviating CTX-induced bone marrow suppression, offering significant clinical application potential. These findings provide a theoretical basis and experimental evidence for developing new adjunct chemotherapy treatment strategies.
Integrated Transcriptome and Proteome Analyses Reveal Differentially Expressed Genes and Proteins in Granulosa Cells from Female Patients with Metabolic Syndrome-associated Infertility
Authors: Dong Fangli , Zhang Wanjun , Sun Bo , Niu Wenbin , Zhai Jun , Guo Yihong , Wang Fang 
Published: January 2025

Background:
Metabolic Syndrome (MS) is a cluster of conditions that significantly
increase the risk of infertility in women. Granulosa cells are crucial for ovarian
folliculogenesis and fertility. Understanding molecular alterations in these cells can provide
insights into MS-associated infertility.

Objective:
This study aimed to investigate Differentially Expressed Genes (DEGs) and
Proteins (DEPs) in granulosa cells from female patients with MS-associated infertility.

Method:
Transcriptome and proteome analyses were integrated to compare granulosa
cells from three MS patients with infertility to three control subjects. RNA sequencing
and quantitative proteomics analyses were conducted, followed by differential expression
analysis, Gene Set Enrichment Analysis (GSEA), and Protein-protein Interaction (PPI)
network construction. Functional enrichment of overlapping DEGs and DEPs and potential
drug-protein interactions were also explored. Hub genes identified by PPI were
validated via quantitative Polymerase Chain Reaction (qPCR) and western blot assays.

Results:
Principal Component Analysis (PCA) demonstrated a distinct separation between
MS and control groups, indicating significant differences in gene and protein expression.
A total of 1,046 upregulated and 23 downregulated DEGs, along with 222 upregulated
and 412 downregulated DEPs, were identified in the MS group. GSEA highlighted
enrichment in processes, like the cell cycle and immune response. Venn diagram
revealed 71 overlapping DEGs and DEPs, mainly related to immune regulation. Key
hub proteins and potential therapeutic candidates were identified, with hub genes upregulated
at the mRNA level, but downregulated at the protein level in granulosa cells of MS
patients.

Conclusion:
The integrative analyses revealed significant molecular alterations in granulosa
cells from MS patients with infertility. Identified DEGs, DEPs, and hub proteins
suggested potential therapeutic targets and pathways for addressing MS-associated infertility.
Plasmalogens Activate AKT/mTOR Signaling to Attenuate Reactive Oxygen Species Production in Spinal Cord Injury
Authors: Cheng Mengdan , Gao Yan , Wu Yiqing , Zhang Liangliang , Xu Bai , Lu Xiaojie 
Published: January 2025

Background:
Plasmalogens, the primary phospholipids in the brain, possess intrinsic antioxidant
properties and are crucial components of the myelin sheath surrounding neuronal axons.
While their neuroprotective effects have been demonstrated in Alzheimer's disease, their potential
benefits in spinal cord injury remain unexplored. This study investigates the reparative effects of
plasmalogens on spinal cord injury and the underlying mechanisms.

Methods:
In vitro, we developed dorsal root ganglion (DRG) and RAW 264.7 cell models under
high-reactive oxygen species (ROS) conditions to assess ROS levels, neuronal damage, and inflammatory
microenvironment changes before and after plasmalogen application. In vivo, we used a
complete mouse spinal cord transection model to evaluate changes in ROS levels, neuronal demyelination,
and apoptosis following plasmalogen treatment. Additionally, we assessed sensory and
motor function recovery and investigated the regulatory effects of plasmalogens on the AKT/mTOR
signaling pathway.

Results:
In high-ROS cell models, plasmalogens protected DRG neurons (TUJ-1) from axonal
damage and modulated the proinflammatory/anti-inflammatory balance in RAW 264.7 cells. In vivo,
plasmalogens significantly reduced ROS levels, improved the immune microenvironment, decreased
the proinflammatory (iNOS)/anti-inflammatory (ARG-1) ratio, lowered neuronal (TUJ-1)
apoptosis (Caspase-3, BAX), and reduced axonal degeneration while promoting myelin (MBP) regeneration,
indicating a neuroprotective effect. These findings are linked to the activation of the
AKT/mTOR signaling pathway.

Conclusion:
Plasmalogens reduce ROS levels and regulate inflammation-induced damage, contributing
to neuroprotection. This study reveals that plasmalogens promote remyelination, reduce axonal
degeneration and neuronal apoptosis, and—used here for the first time in spinal cord injury repair—
may protect neurons by reducing ROS levels and activating the AKT/mTOR signaling pathway.
A Mechanistic Insight into the Effects of Marine Sources as Functional Foods Against Metabolic Syndrome: A Review Article
Authors: Ghasemi Javad , Shamsabadi Shahnam , Mahzoon Erfan , Nazer Yazdan , Rahimi Vafa Baradaran , Askari Vahid Reza 
Published: January 2025

Introduction:
Marine organisms are a rich source of metabolites that can be considered
functional foods and produce new medicines against metabolic syndrome, including hypertension,
dyslipidemia, hyperglycemia, obesity, insulin resistance, and hyperinsulinemia.

Method:
A total of 609 publications were searching results, 283 were screened entirely, and 43 duplications
were removed.

Results:
These compounds, found in marine organisms such as algae, sponges, and mollusks, have
vasodilatory properties, inhibit certain enzymes involved in blood pressure regulation, and exhibit
anti-oxidant activity, making them promising candidates for developing hypertension treatments.
Marine organisms showed an improvement in insulin sensitivity and blood glucose level regulation,
suggesting their potential in developing novel therapies for diabetes management. Furthermore, some
marine sources inhibit lipid accumulation, regulate metabolism, and suppress appetite, indicating
their potential as therapeutic agents in combating obesity.

Conclusion:
However, further studies on the pharmacological effects of marines should be performed
to evaluate the potential of these compounds to produce novel drugs, but they hope this study
paves and lights the way.
The Usability and Effect of a Novel Intelligent Rehabilitation Exergame System on Quality of Life in Frail Older Adults: Prospective Cohort Study
Authors: Chang Chien-Hsiang , Wei Chun-Chun , Lien Wei-Chih , Yang Tai-Hua , Liu Bo , Lin Yimo , Tan Poh Thong , Lin Yang-Cheng 
Published: January 2025

Abstract

Background
Aging in older adults results in a decline in physical function and quality of daily life. Due to the COVID-19 pandemic, the exercise frequency among older adults decreased, further contributing to frailty. Traditional rehabilitation using repetitive movements tends not to attract older adults to perform independently.

Objective
Intelligent Rehabilitation Exergame System (IRES), a novel retro interactive exergame that incorporates real-time surface electromyography, was developed and evaluated.

Methods
Frail older adults were invited to use the IRES for rehabilitation using lower limb training twice per week for 4 weeks. Participants were required to have no mobility or communication difficulties and be willing to complete the 4-week study. The enrolled cohort had baseline scores ranging from 1 to 5 on the Clinical Frailty Scale, as described by Rockwood et al. Three major lower limb movements (knee extension, plantar flexion, and dorsiflexion) were performed 20 times for each leg within 30 minutes. The surface electromyography collected and analyzed muscle potential signals for review by health care professionals to customize the protocol for the next training. The System Usability Scale (SUS) and Taiwanese version of the EuroQol-5 Dimensions (EQ-5D) were administered after completing the first (week 1, baseline) and last training (week 4, one-month follow-up) to evaluate the usability of the IRES and its effects on the quality of life of participants.

Results
A total of 49 frail older adults (mean age 74.6 years) were included in the analysis. The usability of the IRES improved according to the mean SUS score, from 82.09 (good) at baseline to 87.14 (good+) at 1-month follow-up. The willingness to use (t96=−4.51; P<.001), learnability (t96=−4.83; P<.001), and confidence (t96=−2.27; P=.02) in working with the IRES increased. After using the IRES for 1 month, significant improvements were observed in the ease of use (t47=2.05; P=.04) and confidence (t47=2.68; P<.001) among participants without previous rehabilitation experience. No sex-based differences in the SUS scores were found at baseline or 1-month follow-up. The quality of life, as assessed by the EQ-5D, improved significantly after 1 month of IRES training compared to that at baseline (t96=6.03; P<.001).

Conclusions
The novel IRES proposed in this study received positive feedback from frail older adults. Integrating retro-style exergame training into rehabilitation not only improved their rehabilitation motivation but also increased their learning, system operability, and willingness to continue rehabilitation. The IRES provides an essential tool for the new eHealth care service in the post–COVID-19 era.
Exploring Social-Ecological Pathways From Sexual Identity to Sleep Among Chinese Women: Structural Equation Modeling Analysis
Authors: Wu Chanchan , Chau Pui Hing , Choi Edmond Pui Hang 
Published: January 2025

Abstract

Background
Women and sexual minority individuals have been found to be at higher risk for experiencing poor sleep health compared to their counterparts. However, research on the sleep health of sexual minority women (SMW) is lacking in China.

Objective
This study aimed to examine sleep quality and social support for Chinese women with varied sexual identities, and then investigate the in-depth relationships between sexual identity and sleep.

Methods
This was a cross-sectional web-based survey. All participants completed a structured questionnaire containing a set of sociodemographic items referring to the social-ecological model of sleep health, the Pittsburgh Sleep Quality Index, the Social Support Rating Scale, and social relationships and environment domains of the World Health Organization Quality of Life-abbreviated short version. Pearson correlation coefficients were used to examine the relationship between sleep quality and social support as well as the two domains of quality of life. Structural equation modeling analysis was used to explore the social-ecological relationships.

Results
A total of 250 cisgender heterosexual women (CHW) and 259 SMW were recruited from July to September 2021. A total of 241 (47.3%) women experienced poor sleep quality and the rate was significantly higher in SMW than in CHW (55.2% vs 39.2%, P<.001). Around one-fifth of SMW reported low levels of social support, which was significantly higher than that of CHW (21.6% vs 5.6%, P<.001). Pearson correlations showed that overall sleep quality was significantly negatively associated with social support with weak correlations (r=−0.26, P<.001). The final structural equation modeling analysis with satisfactory fit indices identified 6 social-ecological pathways, showing that alcohol use, objective support, utilization of support, and perceived social relationship and environment quality of life played important roles in the sleep quality of individuals from their sexual identity.

Conclusions
SMW experienced poorer sleep quality compared to CHW. Further research is recommended to address the modifiable factors affecting sleep and then implement tailored sleep improvement programs.