bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dyeing of polyamide fabrics with a reverse micellar system using soybean oil as a solvent
Authors: Guimarães Letícia Fantinati , Granato Miguel Angelo 
Published: May 2023

AbstractThe current study reports the results of non‐aqueous dyeing on polyamide fabrics based on a reverse micellar system composed of a stable emulsion of soybean oil, a co‐surfactant, a nonionic surfactant and acid dyes. The system does not make use of water or electrolytes and significantly reduces the dyebath volume. The influence of the hydrophilic lipophilic balance of the surfactants was evaluated, and it was found that the more hydrophilic the surfactant, the better the interaction of the reverse micellar emulsion with the substrate and, consequently, the greater the resulting colour strength. The co‐surfactant plays an important role in the system, as its proportion to the surfactant/oil mixture is directly related to better exhaustion of the dyebath. The results were compared with those from a conventional water‐based dyeing system. Colour matching was performed using the CIELab colour difference formula (ΔE) measured by a reflectance spectrophotometer.
In vitro construction of liver organoids with biomimetic lobule structure by a multicellular 3D bioprinting strategy
Authors: Jian Honglei , Li Xin , Dong Qianqian , Tian Shaonan , Bai Shuo 
Published: May 2023

AbstractLiver disease is one of the serious threats to human life and health. Three‐dimensional (3D) liver models, which simulate the structure and function of natural liver tissue in vitro, have become a common demand in medical, scientific and pharmaceutical fields nowadays. However, the complex cellular composition and multi‐scale spatial arrangement of liver tissue make it extremely challenging to construct liver models in vitro. According to HepaRG preference and printing strategy, the formulation of bioink system with opposite charge is optimized. The sodium alginate‐based bioink 1 and dipeptide‐based bioink 2 are used to ensure structural integrity and provide flexible designability, respectively. The HepaRG/HUVECs/LX‐2‐laden liver organoids with biomimetic lobule structure are fabricated by a multicellular 3D droplet‐based bioprinting strategy, to mimic the cell heterogeneity, spatial structure and extracellular matrix (ECM) features. The liver organoids can maintain structural integrity and multicellular distribution within the printed lobule‐like structure after 7 days of culture. Compared with the 2D monolayer culture, the constructed 3D organoids show high cell viability, ALB secretion and urea synthesis levels. This study provides a droplet‐based and layer‐by‐layer 3D bioprinting strategy for in vitro construction of liver organoids with biomimetic lobule structure, giving meaningful insights in the fields of new drugs, disease modelling, and tissue regeneration.
A novel inducible haematopoietic cell‐depleting mouse model for chimeric complementation of blood cells
Authors: Cao Weiyun , Cao Jiani , Li Xing , Xu Haoyu , Tian Jiayi , Li Xiaoyan , Wang Fengchao , Zhao Tongbiao 
Published: May 2023

AbstractHaematopoietic stem cell transplantation (HSCT) is widely used in regenerative medicine. HSCT can be used not only to treat certain types of blood cancer and immune disorders but also to induce immune tolerance in organ transplantation. However, the inadequacy of HSCs available for transplantation is still a major hurdle for clinical applications. Here, we established a novel inducible haematopoietic cell‐depleting mouse model and tested the feasibility of using chimeric complementation to regenerate HSCs and their progeny cells. Large populations of syngeneic and major histocompatibility‐mismatched haematopoietic cells were successfully regenerated by this model. The stable allogeneic chimeric mice maintained a substantial population of donor HSCs and Tregs, which indicated that the donor allogeneic HSCs successfully repopulated the recipient blood system, and the regenerated donor Tregs played essential roles in establishing immune tolerance in the allogeneic recipients. In addition, rat blood cells were detected in this model after xenotransplantation of rat whole bone marrow (BM) or Lin− BM cells. This mouse model holds promise for regenerating xenogeneic blood cells, including human haematopoietic cells.
Cell spheroids culture array with modifiable chemical gradients
Authors: Yang Panhui , Wu Lei , Zhang Guoyuan , Ge Yuqing , Liu Ting , Mao Hongju , Zhao Jianlong 
Published: May 2023

AbstractCancer cell spheroids have been shown to mimic in vivo tumour microenvironment and are therefore suitable for in vitro drug screening. Microfluidic technology can provide conveniences for spheroid assays such as high‐throughput, simplifying manual operation and saving reagent. Here, we propose a concentration gradient generator based on microfluidic technology for cell spheroid culture and assay. The chip consists of upper microchannels and lower microwells. After partitioning HepG2 suspension into the microwells with concave and non‐adhesive bottoms, spheroids can spontaneously form. By controlling the fluid replacement and flow in microchannels, the doxorubicin solution is diluted automatically into a series of concentration gradients, which spanning more than one order of magnitude. And then the effect of doxorubicin on spheroids is measured in situ by fluorescent staining. This chip provides a very promising approach to achieve the high‐throughput and standardized anti‐cancer drug screening in future.
An aggregation of human embryonic and trophoblast stem cells reveals the role of trophectoderm on epiblast differentiation
Authors: Wu Xulun , Zhao Wentao , Wu Hao , Zhang Qiancheng , Wang Yiming , Yu Kunyuan , Zhai Jinglei , Mo Fan , Wang Meijiao , Li Shiwen , Zhu Xili , Liang Xiaoyan , Hu Baoyang , Liu Guang‐Hui , Wu Jun , Wang Hongmei , Guo Fan , Yu Leqian 
Published: May 2023

AbstractThe interactions between extra‐embryonic tissues and embryonic tissues are crucial to ensure proper early embryo development. However, the understanding of the crosstalk between the embryonic tissues and extra‐embryonic tissues is lacking, mainly due to ethical restrictions, difficulties in obtaining natural human embryos, and lack of appropriate in vitro models. Here by aggregating human embryonic stem cells (hESCs) with human trophoblast stem cells (hTSCs), we revealed the hESCs robustly self‐organized into a unique asymmetric structure which the primitive streak (PS) like cells exclusively distributed at the distal end to the TS‐compartment, and morphologically flattened cells, presumed to be the extra‐embryonic mesoderm cells (EXMC) like cells, were induced at the proximal end to hTSCs. Our study revealed two potential roles of extra‐embryonic trophectoderm in regulating the proper PS formation during gastrulation and EXMCs induction from the human epiblast.
Dominant attitudes and values toward wildlife and the environment in coastal Alabama
Authors: Weber Hertel Sarah , Stupavsky Jana , Alford Kristine , Hicks Hannah R. , Heaton Andrew , Katlein Nathan , Hastings Brandon T. , Stern Adam , Jett Stephanie , Wang Andrew Y. , Wang Bin , Glaberman Scott , Chiari Ylenia 
Published: May 2023

AbstractSurveys assessing attitudes and values about the environment can help predict human behavior toward wildlife and develop effective conservation goals alongside local communities. Coastal Alabama, in the southeastern United States, is a hotspot for biodiversity and endemism and needs protection. Land and wildlife management practices in Alabama have moved from indigenous‐led, which is more in harmony with the environment, to larger‐scale exploitative uses for agriculture and plantations. We therefore predicted that a large proportion of the population has a dominant view of the environment in which land and wildlife are primarily for human benefit. To test this hypothesis, we surveyed over 1300 residents in Mobile and Baldwin counties—the two southernmost counties in Alabama—to assess attitudes toward local vertebrate wildlife, knowledge of the region's biodiversity, and whether individuals value protected areas where they live and/or work. As hunting is generally considered a dominant behavior, we used self‐identified hunters versus non‐hunters to examine the relationship between humans and the environment. Overall, hunters would kill or kill to eat more often than non‐hunters, and would kill even when it is not for food. Furthermore, regardless of hunting status, most participants in our survey would kill a snake, indicating that targeted environmental education is needed for this group. Both hunters and non‐hunters, independently of demographic differences including education and income levels, were not familiar with the especially rich biodiversity of the area and would not be willing to invest money to protect it. Our results indicate that targeted education about the unique and rich biodiversity of southern Alabama compared to the rest of the United States is needed to support successful environmental management, conservation actions, and local participation.
Comparative efficacy of treatments for molluscum contagiosum: A systematic review and network meta‐analysis
Authors: Chao Yuan‐Chen , Ko Mei‐Ju , Tsai Wan‐Chuan , Hsu Le‐Yin , Wu Hon‐Yen 
Published: May 2023

SummaryBackground and objectivesVarious interventions have been applied to treat molluscum contagiosum, but benefits and efficacy remain unclear. To assess the comparative efficacy and safety of interventions for molluscum contagiosum, a network meta‐analysis was performed.Patients and methodsEmbase, PubMed, and the Cochrane Library were searched for articles published between January 1, 1990, and November 31, 2020. Eligible studies were randomized clinical trials (RCTs) of interventions in immunocompetent children and adults with genital/non‐genital molluscum contagiosum lesions.ResultsTwelve interventions from 25 RCTs including 2,123 participants were assessed. Compared with the placebo, ingenol mebutate had the most significant effect on complete clearance (odds ratio [OR] 117.42, 95% confidence interval [CI] 6.37–2164.88), followed by cryotherapy (OR 16.81, 95% CI 4.13–68.54), podophyllotoxin (OR 10.24, 95% CI 3.36–31.21), and potassium hydroxide (KOH) (OR 10.02, 95% CI 4.64–21.64). Data on adverse effects were too scarce for quantitative synthesis.ConclusionsIngenol mebutate, cryotherapy, podophyllotoxin, and KOH were more effective than the other interventions in achieving complete clearance, but safety concerns regarding ingenol mebutate have recently been reported. Due to the possibility of spontaneous resolution, observation is also justified for asymptomatic infection. Factors including adverse effects, cost, patient preference, and medical accessibility should be considered.
Experimental study on thermal fatigue damage and failure mechanisms of basalt exposed to high‐temperature treatments
Authors: Niu Yong , Wang Gang , Wang Jinguo , Liu Xiqi , Zhang Ranran , Qiao Jiaxing , Zhang Jianzhi 
Published: May 2023

AbstractUnderstanding the effects of thermal fatigue damage on the failure mechanisms of rocks is a key concern in underground engineering. The effects of high temperature on the physical–mechanical behaviors and the failure mechanism of basalt under uniaxial compression are investigated with a combination of acoustic emission (AE), computed tomography (CT), and scanning electron microscope (SEM). The high temperature heavily affects the physical–mechanical properties of basalt but has no effect on the mineral compositions. The evolution characteristics of inter‐event time function F(τ) and cumulative AE energy can be employed to characterize the fracture process of thermally damaged basalt. The damage mechanisms of thermal fatigue are attributed to the occurrence of intergranular cracks, intragranular cracks, and transgranular cracks and irregular holes within basalt. The failure mechanisms of basalt change from shear fracture to mixed tensile–shear fracture and finally to tensile fracture based on the statistical characteristics of low and high dominant frequencies.
Incentives, audits and procurement: Evidence from a district‐level field experiment in Ghana
Authors: Denny Elaine K. , Phan Ngoc , Romero Diego , Wibbels Erik 
Published: May 2023

AbstractThere is growing affinity for audits as a tool to promote political accountability and reduce corruption. Nevertheless, knowledge about the mechanisms through which audits work remains limited. While most work on audits shows that they can work via citizen sanctions of bad performers, we emphasize that audit effects can also run through prospective incentives, that is, the desire to avoid poor audit results in the first place. We distinguish audits' impact on prospective incentives and sanctions using a field experiment in Ghana; districts were randomized into audit treatment conditions targeting district procurement and oversight of development projects. We assess the effect of audits on political officials using survey experimental data and show that officials respond powerfully to prospective incentives. In districts treated with top‐down audits, in‐party favoritism falls from 60 percent at baseline to 20 percent at midline, and rates remain at 19 percent at endline. This suggests that the audit's main effect occurred before the audit results were made public, and that prospective mechanisms play an important role in audit efficacy.
Neutrophils extracellular traps and ferroptosis in diabetic wounds
Authors: Huang Yumeng , Ding Youjun , Wang Beizhi , Ji Qian , Peng Chen , Tan Qian 
Published: May 2023

AbstractWound healing is an extremely complex process involving multiple levels of cells and tissues. It is mainly completed through four stages: haemostasis, inflammation, proliferation, and remodelling. When any one of these stages is impaired, it may lead to delayed healing or even transformation into chronic refractory wounds. Diabetes is a kind of common metabolic disease that affects approximately 500 million people worldwide, 25% of whom develop skin ulcers that break down repeatedly and are difficult to heal, making it a growing public health problem. Neutrophils extracellular traps and ferroptosis are new types of programmed cell death identified in recent years and have been found to interact with diabetic wounds. In this paper, the normal wound healing and interfering factors of the diabetic refractory wound were outlined. The mechanism of two kinds of programmed cell death was also described, and the interaction mechanism between different types of programmed cell death and diabetic refractory wounds was discussed.
Analysis of etiological factors of nursing diagnosis of impaired comfort in children and adolescents with cancer
Authors: Leandro Tânia Alteniza , da Silva Viviane Martins , Lopes Marcos Venícios de Oliveira , de Souza Nayana Maria Gomes , Lourenço Penaforte Kiarelle , Gueiros Emanuela Aparecida Teixeira , Nascimento de Oliveira Marisa 
Published: May 2023

AbstractAimTo analyse the etiological factors of the nursing diagnosis of impaired comfort in children and adolescents with cancer.DesignThis cross‐sectional study was carried out in the referral unit for the treatment of childhood cancer in a tertiary hospital located in northeastern Brazil.MethodsA total of 200 children and adolescents who were undergoing cancer treatment were included in this study. Data collection instruments and protocols were constructed with operational and conceptual definitions of clinical indicators and etiological factors for the nursing diagnosis of impaired comfort. A latent class model with adjusted random effects was used to determine impaired comfort and measures of sensitivity and specificity of clinical indicators. A univariate logistic regression analysis was performed for each etiological factor of impaired comfort.ResultsThe analysis of etiological factors for the nursing diagnosis of impaired comfort in children and adolescents with cancer showed the high prevalence of four factors: noxious environmental stimuli, insufficient situational control, insufficient resources and insufficient environmental control. Illness‐related symptoms, noxious environmental stimuli, and insufficient environmental control increased the chance of impaired comfort occurring.ConclusionThe etiological factors with the highest prevalence and most significant impact on the occurrence of impaired comfort were noxious environmental stimuli, insufficient situational control and illness‐related symptoms.ImpactThe results obtained in this investigation can support more accurate nursing diagnostic inference of impaired comfort in children and adolescents with cancer. Moreover, the results can inform direct interventions for the modifiable factors that trigger this phenomenon to avoid or minimize the signs and symptoms of the nursing diagnosis.
‘How is our job affecting us?’ Neonatal Intensive Care Unit healthcare providers and their pregnancy: A descriptive qualitative study
Authors: Dussi Gaia , Bembich Stefano , Crevatin Roberta , Buchini Sara , Schreiber Silvana , Bicego Livia , Cassone Andrea , Dobrina Raffaella 
Published: May 2023

AbstractAimThe aim of this study was to understand the Neonatal Intensive Care Unit (NICU) healthcare providers' (HCPs) experiences and needs during their own past, present and desired pregnancies. It has been reported that HCPs' personal and professional lives are mutually interconnected. Given the NICU HCPs' knowledge of the risks and potential adverse outcomes of newborns admitted to the NICU, their pregnancy experience may be more challenging than that of the general population. However, to date, these aspects are still little studied.DesignA qualitative descriptive study design was adopted.MethodsSemi‐structured interviews were conducted between January and April 2021 in a single third‐level NICU in northeastern Italy. Transcripts were analysed using inductive content analysis. Findings are reported according to the COREQ guidelines.ResultsNineteen HCPs participated in this study. Participants included 12 nurses, 6 medical doctors, and 1 paediatric physical therapist. All participants reported that their professional knowledge and experience influenced their pregnancy‐related experiences, emotions and behaviours. Some participants employed adaptive coping strategies, while others were likely to experience post‐traumatic stress reactions. The narratives of the men and women were similar. Three themes were identified: ‘Feeling different’; ‘Influence of work experience on decision‐making’; ‘Coping with difficulties’.ConclusionTo reduce the potential impact of NICU HCPs' work experience on pregnancy, family functioning and infant health, management of parental emotions should be considered for this population.ImpactHospital managers could prevent the potential distress of vulnerable NICU healthcare workers during pregnancy by tailoring interventions aimed at supporting them in becoming aware and making sense of their work experiences or sensitizing individual psychological support. Moreover, university students should be offered self‐help strategies to face potential dual role conflicts in their future careers.Patient and Public InvolvementNo patient or public contribution.
Mobile collaborative learning with social network awareness
Authors: Lin Jian‐Wei , Huang Hsieh‐Hong , Tsai Chia‐Wen 
Published: May 2023

AbstractBackgroundSocial network awareness (SNA) enables students to know the online learning context of peers in computer‐supported collaborative learning (CSCL) and it has been used to improve peer interaction and participation in collaborative learning tasks. Mobile learning enables students to access learning content and discuss content with peers regardless of location or time. Because almost every college student has their own mobile device and social networks are ubiquitous in their daily lives, a mobile learning application with social networking is promising for promoting effective learning if students are willing to continually use the application. However, few studies have investigated this.ObjectivesIn this study, SNA was integrated into a mobile‐based assessment (MBA–SNA). The study investigated whether students were willing to persist in the use of MBA–SNA and the factors influencing this intention.MethodsA 1‐month experiment was conducted to investigate the research question on the basis of the unified theory of acceptance and use of technology (UTAUT).ResultsThe results demonstrated that (1) students were willing to continue using the MBA–SNA, (2) the incentives influencing behavioural intention (BI) in initial use and continual use differed, and (3) social influence (SI) and perceived playfulness (PP) were the two key determinants of continual use.ImplicationsThe SNA increases the positive impact of SI and PP on MBA continual use so that continual use can be assured. Future research should integrate SNA into other mobile learning apps (e.g., mobile collaborative writing or discussion apps) to enhance continued system use and learning effectiveness.
How policy problems and solutions travel in the scientific literature: An international scientometric analysis of the French Model of opioid use disorder care
Authors: Kankanam Gamage Kasunka , Chiu Kellia , Ryk Justin , Grundy Quinn , Sud Abhimanyu 
Published: May 2023

AbstractRationaleThe 2007 article ‘Why buprenorphine is so successful in treating opiate addiction in France’ has been widely cited to promote various solutions to growing opioid‐related harms across multiple jurisdictions globally. However, selective promotion of aspects of the French experience or promotion of the French experience without considering relevant contextual factors may inform policies that will not bring the same outcomes as in France, including the introduction of possible unintended negative consequences. The scientific literature is one important arena in which policy solutions are identified, evaluated, promoted and disseminated. Scientific communication of the French opioid use disorder care model offers a timely and relevant example through which to examine how problem representations travel and to consider the effects of these representations.Aims and ObjectivesWe aimed to explore where, when, and how the content of this 2007 index article has travelled through the scientific literature.MethodInformed by Bacchi's understanding of problem representation, we conducted a scientometric analysis of the index article. This included categorical analyses using a combination of citation metadata and content data to identify patterns across locations and time.ResultsResearchers in the United States and Anglophone countries affirmatively cited specific index study content, namely less stringent regulations and positive outcomes, such as reductions in overdose deaths and increases in buprenorphine utilization. These citations were more common after 2015 and were more likely to be in discussion sections of nonempirical publications. Researchers from France cited similar content but did so nonaffirmatively, and throughout the study period. Likewise, the French citations were mostly agenda‐setting citations in the introductory sections of empirical studies. US studies received the highest attention based on number of citations and Altmetric scores.ConclusionUS studies, by focusing on less stringent buprenorphine regulation as the primary solution of concern, have constructed opioid‐related harms as a problem of restrictive regulations for buprenorphine. This selective focus on regulation, as opposed to other aspects of the French Model elucidated in the index article such as changes pertaining to the values and financing that structure health service delivery, represents an important missed opportunity for evidence‐informed policy learning across jurisdictions.