bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thrombospondin‐1 mimic peptide PKHB1 induced endoplasmic reticulum stress‐mediated but CD47‐independent apoptosis in non‐small cell lung cancer
Authors: Ye Jiani , Yao Yinan , Zhao Jianfeng , Cui Luyun , Lu Guohua , Wang Qing , Zhang Pei , Zhou Jianying 
Published: January 2023

AbstractNon‐small cell lung cancer (NSCLC) is one of the most common malignancies with high morbidity and mortality. PKHB1, a serum‐stable Thrombospondin‐1 (TSP‐1) mimic peptide, has shown some effective ability in triggering cell death against several cancers. Here, we aimed to study the potential biological function of PKHB1 and its molecular mechanism in NSCLC. Our results revealed that PKHB1 significantly suppressed NSCLC cell proliferation, cell migration, and induced apoptosis in a dose‐dependent manner. Additionally, we found that PKHB1 treatment resulted in mitochondrial transmembrane potential depolarization, Ca2+ overloading as well as the upregulation of proapoptotic proteins. Mechanistically, PKHB1 induced NSCLC cells apoptosis in a CD47‐independent manner. Further study revealed that PKHB1 provoked endoplasmic reticulum (ER) stress principally through the activation of CHOP and JNK signaling, which could be alleviated in the presence of 4‐PBA, an ER stress inhibitor. Furthermore, xenograft tumor models showed that PKHB1 treatment could notably inhibit NSCLC tumor growth in vivo. In conclusion, these findings suggested that PKHB1 exerted antitumor efficacy in NSCLC via triggering ER stress‐mediated but CD47‐independent apoptosis, potentially functioned as a promising peptide‐based therapeutic agent for NSCLC.
“Where do I even start?” Recommendations for faculty diversifying syllabi in ecology, evolution, and the life sciences
Authors: Perrin‐Stowe Tolulope I. N. , Horner Melissa , Coon Jaime J. , Lynch Lauren R. , de Flamingh Alida , Alexander Nathan B. , Golebie Elizabeth , Swartz Timothy M. , Bader Alyssa C. , Halsey Samniqueka J. 
Published: January 2023

AbstractDiversifying curricula is of increasing interest in higher education, including in ecology and evolution and allied fields. Yet, many educators may not know where to start. Here we provide a framework for meeting standard curriculum goals while enacting anti‐racist and anti‐colonial syllabi that is grounded in the development of a sustainable network of educators. In addition to highlighting this professional learning process and sharing the list of resources our group has developed, we provide suggestions to help educators highlight contributions of minoritized groups, explore multiple ways of knowing, and perform critical assessments of foundational views of life and environmental science fields. We further discuss the key classroom dynamics that affect the success of such anti‐racist and anti‐colonial initiatives. The retention and success of minoritized students in ecology and evolution depends on whether we address injustices in our fields. Our hope is that our fellow educators will use this paper to catalyze their own efforts to diversify their courses.
Quantifying inflammation using interleukin‐6 for improved phenotyping and risk stratification in acute heart failure
Authors: Michou Eleni , Wussler Desiree , Belkin Maria , Simmen Cornelia , Strebel Ivo , Nowak Albina , Kozhuharov Nikola , Shrestha Samyut , Lopez‐Ayala Pedro , Sabti Zaid , Mork Constantin , Diebold Matthias , Péquignot Tiffany , Rentsch Katharina , von Eckardstein Arnold , Gualandro Danielle M. , Breidthardt Tobias , Mueller Christian 
Published: January 2023

AbstractAimsSystemic inflammation may be central in the pathophysiology of acute heart failure (AHF). We aimed to assess the possible role of systemic inflammation in the pathophysiology, phenotyping, and risk stratification of patients with AHF.Methods and resultsUsing a novel Interleukin‐6 immunoassay with unprecedented sensitivity (limit of detection 0.01 ng/L), we quantified systemic inflammation in unselected patients presenting with acute dyspnoea to the emergency department in a multicentre study. One‐year mortality was the primary prognostic endpoint. Among 2042 patients, 1026 (50.2%) had an adjudicated diagnosis of AHF, 83.7% of whom had elevated interleukin‐6 concentrations (>4.45 ng/L). Interleukin‐6 was significantly higher in AHF patients compared to patients with other causes of dyspnoea (11.2 [6.1–26.5] ng/L vs. 9.0 [3.2–32.3] ng/L, p < 0.0005). Elevated interleukin‐6 concentrations were independently predicted by increasing N‐terminal pro‐B‐type natriuretic peptide and high‐sensitivity cardiac troponin T, as well as the clinical diagnosis of infection. Among the different AHF phenotypes, interleukin‐6 concentrations were highest in patients with cardiogenic shock (25.7 [14.0–164.2] ng/L) and lowest in patients with hypertensive AHF (9.3 [4.8–21.6] ng/L, p = 0.001). Inflammation as quantified by interleukin‐6 was a strong and independent predictor of 1‐year mortality both in all AHF patients, as well as those without clinically overt infection at presentation (adjusted hazard ratio [95% confidence interval] 1.45 [1.15–1.83] vs. 1.48 [1.09–2.00]). The addition of interleukin‐6 significantly improved the discrimination of the BIOSTAT‐CHF risk score.ConclusionAn unexpectedly high percentage of patients with AHF have subclinical systemic inflammation as quantified by interleukin‐6, which seems to contribute to AHF phenotype and to the risk of death.
Palladium‐Catalyzed Ring‐Opening Addition of Activated Vinyl Cyclopropanes with N‐Tosylhydrazones
Authors: Wang Yiding , Shen Lei , Wang Helin , Luo Yixin , Zeng Qingle 
Published: January 2023

AbstractRing‐opening addition reaction of activated vinyl cyclopropanes with N‐tosylhydrazones in the presence of palladium(0) and triphenylphosphine affords N‐tosylhydrazone butenylmalonate compounds. Aromatic aldehyde‐derived N‐tosylhydrazones produced terminal and internal N‐allylated products, in which the terminal products are the main. While ketone‐derived tosylhydrazones produced only terminal addition products. The transformation relationship of the terminal and internal N‐allylated products in the reaction was also observed. A reasonable mechanism is proposed based on preliminary experimental results.
Differential pulse voltammetric determination of selected nitrophenols on novel type of porous silver working electrode prepared by powder metallurgy
Authors: Bountas Georgios , Vyskocil Vlastimil , Kratochvil Bohumil , Michalcova Alena , Economou Anastasios , Barek Jiri 
Published: January 2023

AbstractNitrophenols are important environmental pollutants and their monitoring is important because of their genotoxic and ecotoxic properties. Easy electrochemical reduction of nitro groups can be used for their voltammetric determination using mercury based electrodes. However, requirements of green analytical chemistry prompted us to investigate a novel type of silver porous electrode (AgPE) prepared by powder metallurgy compatible with both “green” and “white” analytical chemistry requirements. In this paper, AgPE was for the first time successfully used for differential pulse voltammetric determination of micromolar concentrations of 2‐nitrophenol (NP), 2,4‐dinitrophenol (DNP), and 2,4,6‐trinitrophenol (TNP) in aqueous media. The main advantage of the novel method is the possibility to use small sample volume (down to 25 μL) and to work in the presence of oxygen when using supporting electrolyte of pH 3. This advantage partially compensates the fact that the obtained sensitivity and limit of detection are not better than with the previously investigated electrodes.
Sensitive adsorptive stripping voltammetric procedure of Imatinib determination in spiked urine samples using solid lead microelectrode
Authors: Gęca Iwona , Korolczuk Mieczyslaw 
Published: January 2023

AbstractThis article reports for the first time the application of solid lead microelectrode for organic compound determination. The proposed sensor was used for anticancer drug Imatinib quantification by adsorptive stripping voltammetry. Procedure of Imatinib determination was developed utilizing advantages ensured by solid lead microelectrode: reusability and durability for a long period of time and its ecological character as compared to film electrodes. The detection limit of Imatinib determination was calculated to be 1.9 × 10−10 mol L−1. The analytical usability of the developed procedure was confirmed by acceptable recoveries of Imatinib determination in spiked urine samples.
Influence of the Crystallinity of Silver Nanoparticles on Their Magnetic Properties
Authors: Lin Long , Peng Xiaogang , Voirin Emilie , Donnio Bertrand , Rastei Mircea V. , Vileno Bertrand , Gallani Jean‐Louis 
Published: January 2023

AbstractThe magnetic properties of noble‐metal nanoparticles are a puzzling phenomenon, tentatively often explained as a size effect or a ligand effect. Many experimental studies performed to date have attempted to vary these readily available parameters without reaching a definitive conclusion. In an attempt at better understanding the role of core crystallinity on these magnetic properties, we have compared the behavior of silver nanoparticles, which were either single‐crystalline or multi‐twinned, of almost identical sizes and with the same ligand coating. Our results indicate that single‐crystalline nanoparticles tend to behave as classical paramagnetic materials, whereas multi‐twinned ones exhibit a combination of para‐ and ferro‐magnetic behaviors. Our hypothesis is that lattice defects within the core bear magnetic moments which couple through conduction electrons, with dipolar interactions also playing a local and macroscopic role.
Financial conflicts of interest of physicians followed by neurosurgical journals on Twitter
Authors: Powell Kerrington , McCall Kyle , Hooda Karan , Prasad Vinay , Kakkilaya Akash 
Published: January 2023

AbstractBackgroundSocial media, particularly Twitter, has played an increasing role in networking and the dissemination of neurosurgical research. Despite extensive study on financial conflicts of interest (FCOI) influencing medical research, little is known about the function of conflicts of interest on social media and the influence they may have. In this study, we sought to evaluate the FCOI of physicians followed on Twitter by the top three neurosurgical journals.Materials and MethodsWe analysed the FCOI of United States (US) physicians followed by the top three neurosurgical journals (Journal of Neurosurgery, World Neurosurgery, Neurosurgery) on Twitter. We determined the FCOIs of each physician using the Open Payments Search Tool located at https://openpaymentsdata.cms.gov and summed the data between 2014 and 2021.ResultsWe examined 2651 Twitter accounts followed by the top three neurosurgical journals on Twitter and determined 705 (26.6%) belonged to US physicians. Of the 705 US physicians, 577 (81.8%) received general payments between 2014 and 2021. After excluding US physicians currently in residency or fellowship (n = 157), this percentage increased to 93.2% (n = 511/548). In total, nearly $70 million in general payments were made between 2014 and 2021.ConclusionThese findings raise questions regarding the interaction between neurosurgical journals and the medical community on Twitter. This study may serve as the basis for future work on best practices for medical journals navigating their affiliations on Twitter.
Coinfections with SARS‐CoV‐2 variants and influenza virus during the 2019 Coronavirus disease pandemic in Burkina Faso: A surveillance study
Authors: Lingani Moussa , Cissé Assana , Tialla Dieudonné , Ilboudo Abdoul Kader , Savadogo Madi , Sawadogo Catherine , Gampini Sandrine , Tarnagda Grissoum , Tao Maria , Diagbouga Serge , Bamba Sanata , Tarnagda Zekiba 
Published: January 2023

AbstractBackground and AimSevere acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) particularly the variants of concern coinfections with influenza is a public health concern in Africa. We aimed to characterize the SARS‐CoV‐2 variants and determine the rate of coinfections with influenza in Burkina Faso.MethodsCOVID‐19 surveillance study was conducted between August 2021 and January 2022 using reverse transcription polymerase chain reaction (RT‐PCR). Positive specimens were further screened for SARS‐CoV‐2 variants using the multiple variants real‐time PCR kits. In addition, influenza virus strains were detected by RT‐PCR in SARS‐CoV‐2 positive specimens using the CDC primers, probes, and protocols.ResultsOf 324 specimens assessed, the Omicron and Delta variants of SARS‐CoV‐2 were the most prevalent with 27.2% [95% confident interval (CI): 22.5–32.4] and 22.2% [95% CI: 17.9–27.2], respectively. The Beta and Gamma variants were detected in 4.3% [95% CI: 2.4–7.1] and 0.3% [95% CI: 0.0–1.7], respectively. Coinfections of Omicron and Beta variants were reported in 21.3% [95% CI: 17.0–26.2], Omicron and Delta variants in 1.2% [95% CI: 0.3–3.1] of specimens, and the Omicron–Gamma variants' coinfections in 0.6% [95% CI: 0.1–2.2]. One COVID‐19 specimen with an undetected SARS‐CoV‐2 variant was also tested positive for the seasonal influenza A (H3N2) virus. No cases of pandemic influenza A (H1N1)pdm09, seasonal A/H1N1, and influenza B were detected.ConclusionsThe current World Health Organization SARS‐CoV‐2 variants of concern were prevalent and their coinfections with influenza were uncommon. Continuous surveillance of both pathogens is, however, needed because of their public health implications.
Can a robot lie? Young children's understanding of intentionality beneath false statements
Authors: Peretti Giulia , Manzi Federico , Di Dio Cinzia , Cangelosi Angelo , Harris Paul L. , Massaro Davide , Marchetti Antonella 
Published: January 2023

AbstractIncluding robots in children's lives calls for reflection on the psychological and moral aspects of such relationships, especially with respect to children's ability to differentiate intentional from unintentional false statements, that is, lies from mistakes. This ability calls for an understanding of an interlocutor's intentions. This study examined the ability of 5‐6‐year‐olds to recognize, and morally evaluate, lies and mistakes produced by a human as compared to a NAO robot, and to attribute relevant emotions to the deceived party. Irrespective of the agent, children had more difficulty in understanding mistakes than lies. In addition, they were disinclined to attribute a lie to the robot. Children's age and their understanding of intentionality were the strongest predictors of their performance on the lie‐mistake task. Children's Theory of Mind, but not their executive function skills, also correlated with their performance. Our findings suggest that, regardless of age, a robot is perceived as an intentional agent. Robot behaviour was more acceptable for children because his actions could be attributed to someone who programmed it to act in a specific way.HighlightsThe ability to recognize an intention to lie or not in different agents represents a significant developmental step.Children saw a human/robot making intentionally or unintentionally false statements, and understanding the mistake was more difficult than the lie.Robots may be associated with the human concept by younger children with important implications for use of cHRI in education.
Current testing programs for pesticides adequately capture endocrine activity and adversity for protection of vertebrate wildlife
Authors: Staveley Jane P. , Freeman Elaine L. , McArdle Margaret E. , Ortego Lisa S. , Coady Katherine K. , Bone Audrey , Lagadic Laurent , Weltje Lennart , Weyers Arnd , Wheeler James R. 
Published: January 2023

Abstract
The toxicity and ecotoxicity of pesticide active ingredients are evaluated by a number of standardized test methods using vertebrate animals. These standard test methods are required under various regulatory programs for the registration of pesticides. Over the past two decades, additional test methods have been developed with endpoints that are responsive to endocrine activity and subsequent adverse effects. This article examines the available test methods and their endpoints that are relevant to an assessment of endocrine-disrupting properties of pesticides. Furthermore, the article highlights how weight-of-evidence approaches should be applied to determine whether an adverse response in (eco)toxicity tests is caused by an endocrine mechanism of action. The large number of endpoints in the current testing paradigms for pesticides make it unlikely that endocrine activity and adversity is being overlooked. Integr Environ Assess Manag 2023;19:1089–1109. © 2023 Bayer CropScience and The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Toxicological evaluation of protein powder derived from Cupriavidus necator
Authors: Modica Vickie , Glávits Róbert , Murbach Timothy S. , Endres John R. , Hirka Gábor , Vértesi Adél , Béres Erzsébet , Pasics Szakonyiné Ilona 
Published: January 2023

AbstractMicroorganisms have the potential to produce nutrient‐rich products that can be consumed as food or feed. The protein‐rich powder derived from heat treatment of the whole‐cell biomass of polyhydroxybutyrate‐deficient Cupriavidus necator, a metabolically versatile organism that uses elements found in the air, is an example of such a product. To assess the safety of the protein powder for use as a nutritional ingredient in human food, in accordance with internationally accepted standards, its genotoxic potential and repeated‐dose oral toxicity were investigated. A bacterial reverse mutation test, an in vitro mammalian chromosomal aberration test, and an in vivo mammalian micronucleus test were performed. No evidence of mutagenicity or genotoxicity was found. Additionally, a 90‐day repeated‐dose oral toxicity study in rats was completed, in which a total of 100 male and female Wistar rats were exposed by gavage to daily doses of 1000, 2000, or 3000 mg/kg bw/day of the test material. Following 90 days of continuous exposure, no mortality or treatment‐related adverse effects were observed and no target organs were identified. Therefore, a no observed adverse effect level was determined at 3000 mg/kg bw/day, the highest dose tested.
Elective genomic testing: Practice resource of the National Society of Genetic Counselors
Authors: Blout Zawatsky Carrie L. , Bick David , Bier Louise , Funke Birgit , Lebo Matthew , Lewis Katie L. , Orlova Ekaterina , Qian Emily , Ryan Lauren , Schwartz Marci L. B. , Soper Emily R. 
Published: January 2023

AbstractGenetic counseling for patients who are pursuing genetic testing in the absence of a medical indication, referred to as elective genomic testing (EGT), is becoming more common. This type of testing has the potential to detect genetic conditions before there is a significant health impact permitting earlier management and/or treatment. Pre‐ and post‐test counseling for EGT is similar to indication‐based genetic testing. Both require a complete family and medical history when ordering a test or interpreting a result. However, EGT counseling has some special considerations including greater uncertainties around penetrance and clinical utility and a lack of published guidelines. While certain considerations in the selection of a high‐quality genetic testing laboratory are universal, there are some considerations that are unique to the selection of a laboratory performing EGT. This practice resource intends to provide guidance for genetic counselors and other healthcare providers caring for adults seeking pre‐ or post‐test counseling for EGT. Genetic counselors and other genetics trained healthcare providers are the ideal medical professionals to supply accurate information to individuals seeking counseling about EGT enabling them to make informed decisions about testing and follow‐up.
The synthesis of novel 2, 3‐disubstituted 1,6‐dihydropyrrolo[2,3‐g]indazole derivatives via one‐pot three component reactions
Authors: Chen Sai , Wu Shuang‐Ling , Liu Jia‐Yan , Chen Dong‐Sheng 
Published: January 2023

AbstractA concise and efficient synthetic approach was developed for the synthesis of functionalized 2,3‐disubstituted 1,6‐dihydropyrrolo[2,3‐g]indazole via a one‐pot, three‐component reaction of 1H‐indazol‐6‐amine, arylglyoxal monohydrates, and 4‐hydroxy‐2H‐chromen‐2‐one or 4‐hydroxyquinolin‐2(1H)‐one promoted by acetic acid in ethanol under reflux conditions. The attractive features of this protocol are operational simplicity, short reaction time, easy purification of products, and good to excellent yields.
An innovative approach to career development and promotion of diverse faculty
Authors: Jones Danielle , Fluker Shelly‐Ann N. , Walker Tiffany A. , Manning Kimberly D. , Bussey‐Jones Jada C. 
Published: January 2023

AbstractWomen and persons from racial and ethnic populations underrepresented in medicine (URiM) comprise a substantially lower proportion of academic internal medicine faculty, particularly at senior ranks (associate professors and professors). Numerous factors lead to this inequity which has broad implications for medical education and healthcare. The Emory University Division of General Internal Medicine Grady Section (GIMG) formed the Faculty Review Committee (FRC) in 2013 to address low promotion rates to senior ranks as part of a strategy to foster a more inclusive, equitable environment. The FRC systematically and proactively reviews all GIMG faculty years prior to possible promotion to provide tailored recommendations to bolster professional development and with a goal to expedite successful advancement to senior ranks. Deidentified GIMG academic rank data was compared with aggregate data from Emory University School of Medicine and the American Association of Medical Colleges. In 2020, GIMG had significantly more senior faculty compared with pre‐FRC intervention (odds ratio [OR]: 3.94, 95% confidence interval [CI]: 1.65–9.42). Subgroup analyses of non‐URiM women GIMG senior faculty compared with preintervention (OR: 11.6, 95% CI: 2.52–53.7), showed a significant increase. A trend toward increased URiM women faculty was also seen. Descriptive analysis suggests that the GIMG group had a higher promotion to senior ranks among women and URiM compared with national and institutional comparators. The FRC is associated with significant increases in the promotion of all faculty and non‐URiM women faculty, and an increasing trend of URiM women faculty, effects which help contribute to an equitable academic medicine environment, fostering a more diverse workforce and improved patient outcomes.