bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purpureocillium lilacinum strain AUMC 10620 as a biocontrol agent against the citrus nematode Tylenchulus semipenetrans under laboratory and field conditions
Authors: El-Marzoky Amr M. , Elnahal Ahmed S. M. , Jghef Muthana M. , Abourehab Mohammed A. S. , El-Tarabily Khaled A. , Ali Mohamed A. M. S. 
Published: May 2023

AbstractThree concentrations (1.25, 2.5, and 5 × 107 spores ml−1) (of the biocontrol fungus Purpureocillium lilacinum (strain AUMC 10620) were tested on citrus nematode Tylenchulus semipenetrans under in vitro and field conditions. Larvae and eggs were exposed to the fungal spores in vitro for 24, 48, and 72 h, and the findings were recorded at each time point. These results were compared with the application of the nematicide abamectin. Strain AUMC 10620 effectively reduced larval activity and egg hatching of T. semipenetrans under laboratory conditions. The highest concentration (5× 107 spores ml−1) of P. lilacinum, resulted in 89.01% immobility in the larvae, compared to abamectin, which resulted in 65.93% immobility after 48 h of exposure. These percentages of immobility were increased after 72 h of exposure (100 and 85.09%) when P. lilacinum at a concentration of 5 × 107 spores ml−1 and abamectin were used, respectively. On the other hand, the two other P. lilacinum concentrations (1.25, and 2.5 × 107 spores ml−1) affected the T. semipenetrans larvae to a lesser extent. The highest fungal concentration 5× 107 spores ml−1 inhibited the hatching of T. semipenetrans eggs in vitro with 71.34, 80, and 86.67% after 24, 48 and 72 h of treatment compared to the abamectin treatment which showed 76.67, 78, and 87% after the abovementioned periods, respectively. In addition, the application of P. lilacinum (5 × 107 spores ml−1) or abamectin under field conditions significantly (P < 0.05) reduced the population of the major nematode species (T. semipenetrans, Tylenchorhynchus spp., Helicotylenchus spp., and Pratylenchus spp.) infesting citrus after one, two, and three weeks of treatment compared to the control treatment but with no significant (P > 0.05) differences between the two treatments. Three weeks after the field application, the percentage of nematode reduction was significantly (P < 0.05) smaller than the control treatment at concentrations of 5, 2.5, and 1.25 × 107 spores ml−1, respectively, by 78.42, 64.03, and 58.35%. It is evident from these results that the application of P. lilacinum strain (AUMC 10620) can be used in integrated pest management programs to control nematodes infesting citrus trees.
Graphical abstract
Effects of Temperature and Advanced Cleaning Practices on the Removal of Select Organic Chemicals from Structural Firefighter Gear
Authors: Forester Crystal D. , Tarley Jay 
Published: May 2023

AbstractThere are approximately 1.2 million firefighters in the United States. In addition to fighting fires, they also participate in various tasks including emergency rescues, providing emergency medical care, driving, operating and maintaining fire vehicles and other apparatuses and continued training. During the course of their duties, firefighters are often exposed to carcinogenic chemicals that may accumulate on their turnout clothing. To determine if these chemicals can be removed and to measure the efficacy of current cleaning protocols, a study was developed to assess these protocols which call for a maximum water temperature of 105°F and no solvents. The results showed that volatile and small semi-volatile chemicals such as dimethyl phthalate and chlorinated phenols had cleaning efficiencies of 85% or greater. However, for polycyclic aromatic hydrocarbons (PAHs), the cleaning efficiency was only 20% to 50%. To determine if it is possible to remove the more persistent PAHs, temperatures were increased to 125°F and 140°F, and a respective increase in cleaning efficiencies of the PAHs was seen. The protocols were then adapted to add a pre-soak cycle which, when combined with the increased temperatures, resulted in an increase of the PAH cleaning efficiency range from 50 to 80%, compound dependent. This work provides a starting point for discussions between the National Fire Protection Association (NFPA) and other standards organization and manufacturers to determine if increased temperatures and chemical soaks would be detrimental to gear as they are currently not advised.
Assessment of the salinity tolerance, response mechanisms and nutritional imbalance to heterogeneous salt supply in Populus alba L. clone ‘Marte’ using a split-root system
Authors: Della Maggiora Lorenzo , Francini Alessandra , Giovannelli Alessio , Sebastiani Luca 
Published: May 2023

AbstractSalinity responses of Populus alba L. clone ‘Marte’ were evaluated using a split-root system to simulate the heterogeneous soil conditions in the field and to assess the salinity tolerance of clone ‘Marte’. During the 26 days of experiment, we measured morpho-physiological parameters as well as the mineral content in substrate and plant organs in split-root plants watered with no salt on both sides of the system (0/0 mM - S1), salt on one side (0/100 mM – S2) and salt on both sides (100/100 mM – S3). Shoot elongation, stem diameter increase, and number of new leaves were not significantly different among and within each salt treatment. Leaf relative chlorophyll content and chlorophyll a fluorescence did not show any difference, while there was a decrease of 85% in terms of net photosynthesis (Pn) and 88% in stomatal conductance (gs) in S3 plants compared to S1. Na, absorbed by one side of the 0/100 mM plants, was equally distributed in all aerial organs and reached a concentration in-between the other two groups, as a confirmation of the physiological results obtained. An increase in Mn concentrations in woody cutting, stems and leaves of S2 and S3 system compared to S1 group was observed. Moreover, S3 plants showed an increase of K concentration in leaves. In ‘Marte’ poplar, Na increment was only translated in a reduction of photosyntetic performances, and the increase of some key mineral elements at leaf level such as K and Mn suggested an attempt to overcome the stress.
Focal pulsed field ablation and ultrahigh-density mapping — versatile tools for all atrial arrhythmias? Initial procedural experiences
Authors: Ruwald Martin H. , Johannessen Arne , Hansen Morten Lock , Haugdal Martin , Worck Rene , Hansen Jim 
Published: May 2023

Abstract

Background
Focal pulsed field ablation (FPFA) is a novel and promising method of cardiac ablation. The aim of this study was to report the feasibility, short-term safety, and procedural findings for a broad spectrum of ablated atrial arrhythmias.

Methods
Patients (n = 51) scheduled for ablation of atrial arrhythmias were prospectively included and underwent FPFA using the Galvanize CENTAURI generator with energy delivery through commercially available ablation catheters with ultrahigh-density (UHDx) 3D electroanatomic voltage/local activation time map evaluations. Workflow, procedural data, and peri-procedural technical errors and complications are described.

Results
Planned ablation strategy was achieved with FPFA-only in 48/51 (94%) of the cases. Ablation strategy was first-time pulmonary vein isolation (PVI) in 17/51 (36%), repeat ablation in 18/51 (38%), PVI + in 13/51 (28%), and cavotricuspid isthmus block (CTI)-only in 3/51 (6%). The mean procedure time was 104 ± 31 min (first-time PVI), 114 ± 26 min (repeat procedure), 152 ± 36 min (PVI +), and 62 ± 17 min (CTI). Mean UHDx mapping time to assess lesion formation and block after ablation was 7 ± 4 min with 5485 ± 4809 points. First pass acute (linear) isolation with bidirectional block for anatomical lesion sets was 120/124 (97%) for all PVs, 17/17 (100%) for (any) isthmus, and 14/17 (82%) for left atrium posterior wall (LAPW). We observed several time-consuming integration errors with the used ablation system (mean 3.4 ± 3.7 errors/procedure), one transient inferior ST elevation when ablating CTI resolved by intravenous nitroglycerine and one transient AV block requiring temporary pacing for > 24 h.

Conclusions
FPFA was a highly versatile method to treat atrial arrhythmias with high first-pass efficiency. UHDx revealed acute homogenous low-voltage lesions in ablated areas. More data is needed to establish lesion durability and limitations of FPFA.
The effects of digital servitization on business competitiveness: A case study of Spanish manufacturers
Authors: Minaya Pedro E. , Avella Lucía , Trespalacios Juan A. 
Published: May 2023

Abstract
In recent years, interest has grown in servitization strategies as business models for selling combined packages of goods and services to create competitive advantage to provide great customer value. Various studies have examined the benefits servitization offers to both businesses and their customers; however, there is very little research on the moderating role played by the digital enablers of Industry 4.0 on servitization. It is this research gap that the present study aims to fill. Using a framework developed from the extant literature, case studies, with an exploratory objective, were conducted to the aim of identifying and analyzing the challenges and opportunities that could be presented in those companies that decide to develop digital servitization. To achieve it, this study focuses on a deep analysis of the experience in five manufacturing companies, and, from that, we draw significant conclusions (both for further research and for business practice) about the strategy of servitization and the moderating role of Industry 4.0 on business competitiveness. We first carried out detailed interviews with key personnel from companies, complementing that with additional information from various sources before analyzing the results to identify emerging topics. The qualitative study allowed us to highlight how the different kinds of digital technologies supported servitization and product innovation. In addition, it provides empirical evidence about the different servitization strategies and Industry 4.0 digital enablers, which, in combination, allows companies achieving increased competitiveness, generate greater returns, find new customers, access new markets, and develop new business ideas.
Revascularization and angiogenesis for bone bioengineering in the craniofacial region: a review
Authors: AL-Fotawi Randa , Fallatah Waleed 
Published: May 2023

AbstractThe revascularization of grafted tissues is a complicated and non-straightforward process, which makes it challenging to perform reconstructive surgery for critical-sized bone defects. This challenge is combined with the low vascularity that results from radiotherapy. This low vascularity could result from ischemia–reperfusion injuries, also known as ischemia which may happen upon grafting. Ischemia may affect the hard tissue during reconstruction, and this can often cause resorption, infections, disfigurement, and malunion. This paper therefore reviews the clinical and experimental application of procedures that were employed to improve the reconstructive surgery process, which would ensure that the vascularity of the tissue is maintained or enhanced. It also presents the key strategies that are implemented to perform tissue engineering within the grafted sites aiming to optimize the microenvironment and to enhance the overall process of neovascularization and angiogenesis. This review reveals that the current strategies, according to the literature, are the seeding of the mature and progenitor cells, use of extracellular matrix (ECM), co-culturing of osteoblasts with the ECM, growth factors and the use of microcapillaries incorporated into the scaffold design. However, due to the unstable and regression-prone capillary structures in bone constructs, further research focusing on creating long-lasting and stable blood vessels is required.
Graphical Abstract
Eigen Background Subtraction for Industrial Flaw Detection: Application to High-Pressure Turbine Blade CT Scans
Authors: Remacha C. , Redoulès G. , Aublet A. 
Published: May 2023

AbstractWe propose a state-of-the-art approach that is the first to use Eigen background subtraction to reveal flaws in three-dimensional Computed Tomography data. Our method is composed of two main steps. During the first step, principal component analysis (PCA) is applied on flaw-free blade stack data. From a statistical perspective, a series of “flaw-free” characteristic functions is extracted. The second step consists of decomposing the blade of interest according to the functions calculated from PCA. This projection allows the construction of a synthetic blade without any flaws. A subtraction between the synthetic blade and real blade highlights the abnormal variations. The main advantage of this technique is that the processing remains applicable even when the measurement system or parts of the system have variability that is greater than the flaw size.
Liquid pressure fluctuations around a needle during bubble departures
Authors: Dzienis P. , Mosdorf R. 
Published: May 2023

AbstractThe bubble–bubble interaction and bubble–needle interaction are usually caused by liquid flow in the needle or the needle’s neighborhood. Moreover, nonlinear changes in the liquid’s velocity and pressure can lead to chaotic bubble departures. In the present paper, the liquid pressure changes in the needle’s neighborhood were experimentally and numerically investigated. In the experiment, bubbles were generated from a brass needle into distilled water. A second needle was placed at a distance of 1.5 mm from the needle which was used to generate the bubble and was used to measure the changes of liquid pressure during successive stages of bubble departures. It was shown that, during the bubble waiting time, the liquid pressure in the needle’s neighborhood fluctuates slightly. Significant changes in the liquid’s pressure were observed during the bubble growth stage. The influence of liquid pressure and liquid velocity above the needle on the bubble growing stage and bubble waiting time was discussed.
A review on the role of NDRG1 in different cancers
Authors: Ghafouri-Fard Soudeh , Ahmadi Teshnizi Sara , Hussen Bashdar Mahmud , Taheri Mohammad , Sharifi Guive 
Published: May 2023

AbstractNDRG1 is a member of the α/β hydrolase superfamily that resides in the cytoplasm and participates in the stress responses, hormone response, cell growth, and differentiation. Several studies have pointed to the importance of NDRG1 in the carcinogenesis. This gene has been found to be up-regulated in an array of cancer types such as bladder, esophageal squamous cell carcinoma, endometrial, lung and liver cancers, but being down-regulated in other types of cancers such as colorectal, gastric and ovarian cancers. The current study summarizes the evidence on the role of NDRG1 in the carcinogenic processes in different types of tissues.
Natural killer cells strengthen antitumor activity of cisplatin by immunomodulation and ameliorate cisplatin-induced side effects
Authors: Wang Zhu , Yang Zhan , Qu Changbao , Li Jinmin , Wang Xiaolu 
Published: May 2023

Abstract
Purpose
Cisplatin-based chemotherapy is now an important treatment for improving bladder cancer prognosis. However, challenges in clinical treatment remain due to the numerous side effects of chemotherapy. Natural killer (NK) cells regulate certain immune responses and play a significant role in tumor surveillance and control. The efficacy of NK cells combined with cisplatin for chemoimmunotherapy in bladder cancer remains poorly understood.

Methods
In this study, we established an MB49 tumor-bearing mouse model, tumor growth was measured in a control group and in groups treated with cisplatin, NK cells or both. Organ indices, biochemical indicators of blood serum, and expression of apoptotic proteins were used to assess the extent of organ damage. ELISA and immunohistochemistry were used to analyze the levels of immune cells and cytokine expression in serum, spleen, and tumor tissue.

Results
NK cells combined with cisplatin exhibited better antitumor activity. NK cells also alleviated the organ damage caused by cisplatin and improved the survival rate. Treatment with NK cells increased the expression of IL-2 and IFN-γ as well as the number of CD4 + T cells. Additionally, cisplatin increased the expression of natural killer group 2, member D (NKG2D) ligands thus activating NK cells to kill tumor cells.

Conclusion
NK cells could alleviate the side effects of cisplatin treatment and enhance antitumor activity. The combination of NK cells and cisplatin thus provides a promising option for chemoimmunotherapy for bladder cancer.
A comparative metabolomics investigation of flavonoid variation in faba bean flowers
Authors: Elessawy Fatma M. , Hughes Jessa , Khazaei Hamid , Vandenberg Albert , El-Aneed Anas , Purves Randy W. 
Published: May 2023

Abstract
Introduction
Faba bean (Vicia faba L.) flowers are edible and used as garnishes because of their aroma, sweet flavor and attractive colors. Anthocyanins are the common plant pigments that give flowers their vivid colors, whereas non-anthocyanin flavonoids can serve as co-pigments that can modify the color intensity of flowers.

Objectives
To explore the polyphenol diversity and differences in standard and wing petals of faba bean flowers; and identify glycosylated flavonoids that contribute to flower color.

Methods
Flower standard and wing petals from 30 faba bean genotypes (eight color groups with a total of 60 samples) were used for polyphenol extraction. Samples were analyzed using a targeted method and a semi-untargeted analysis using liquid chromatography–high resolution mass spectrometry (LC–HRMS) combined with photodiode array (PDA) detection. Compound Discoverer software was used for polyphenol identification and multivariate analysis.

Results
The semi-untargeted analysis guided by the PDA detected 90 flavonoid metabolites present in faba bean flower petals. Ten anthocyanins largely influenced the flower colors, but other flavonoids (63 flavonols and 12 flavones) found with variable levels in different flower color groups appeared to also influence color, especially in mixed colors.

Conclusion
Analysis of the different colored faba bean flowers confirmed that the color variation between the flowers was mainly controlled by anthocyanins in brown, red and purple-red flowers. Of the other flavonoids, multiglycosylated kaempferols were abundant in white and brown flowers, monoglycosylated kaempferols were common in red and purple-red flowers, and quercetin and apigenin glycosides were abundant co-pigments in purple-red flowers.
Sustainable hierarchical multi-modal hub network design problem: bi-objective formulations and solution algorithms
Authors: Nasiri Mohammad Mahdi , Khaleghi Amir , Govindan Kannan , Bozorgi-Amiri Ali 
Published: May 2023

AbstractThis paper presents a bi-objective model for the design and optimization of a sustainable hierarchical multi-modal hub network. The proposed model focuses on sustainability by considering economic, environmental, and social aspects of the decisions in a hierarchical network. A case of Turkish network for freight transportation is used to validate the proposed model. To solve the small-sized problems, the augmented epsilon constraint method version 2 (AUGMECON2) is applied. It can be inferred from the Pareto-optimal set obtained by AUGMECON2 that the effect of increasing the number of hubs after a threshold is marginal. The current contribution proposes two multi-objective genetic algorithms (NSGA-II and NRGA), which incorporate LP solving and Dijkstra algorithm. The results show the superiority of NRGA compared to NSGA-II in terms of solution time. Also, we present an alternative, more efficient formulation to the problem. Based on the alternative formulation, in addition to AUGMECON2, we use two exact methods, including Torabi and Hassini (TH) method and augmented weighted Tchebycheff procedure (AWTP), to find Pareto-optimal solutions for small, medium, and large-sized problems (including the case study). The performance of the proposed solution methods is measured using some multi-objective indicators. The results show the superiority of AUGMECON2.
Tissue Outcome Prediction in Patients with Proximal Vessel Occlusion and Mechanical Thrombectomy Using Logistic Models
Authors: Welle Florian , Stoll Kristin , Gillmann Christina , Henkelmann Jeanette , Prasse Gordian , Kaiser Daniel P. O. , Kellner Elias , Reisert Marco , Schneider Hans R. , Klingbeil Julian , Stockert Anika , Lobsien Donald , Hoffmann Karl-Titus , Saur Dorothee , Wawrzyniak Max 
Published: May 2023

AbstractPerfusion CT is established to aid selection of patients with proximal intracranial vessel occlusion for thrombectomy in the extended time window. Selection is mostly based on simple thresholding of perfusion parameter maps, which, however, does not exploit the full information hidden in the high-dimensional perfusion data. We implemented a multiparametric mass-univariate logistic model to predict tissue outcome based on data from 405 stroke patients with acute proximal vessel occlusion in the anterior circulation who underwent mechanical thrombectomy. Input parameters were acute multimodal CT imaging (perfusion, angiography, and non-contrast) as well as basic demographic and clinical parameters. The model was trained with the knowledge of recanalization status and final infarct localization. We found that perfusion parameter maps (CBF, CBV, and Tmax) were sufficient for tissue outcome prediction. Compared with single-parameter thresholding-based models, our logistic model had comparable volumetric accuracy, but was superior with respect to topographical accuracy (AUC of receiver operating characteristic). We also found higher spatial accuracy (Dice index) in an independent internal but not external cross-validation. Our results highlight the value of perfusion data compared with non-contrast CT, CT angiography and clinical information for tissue outcome-prediction. Multiparametric logistic prediction has high potential to outperform the single-parameter thresholding-based approach. In the future, the combination of tissue and functional outcome prediction might provide an individual biomarker for the benefit from mechanical thrombectomy in acute stroke care.
Electroconductive poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticle-loaded silk fibroin biocomposite conduits for peripheral nerve regeneration
Authors: Escobar Ane , Serafin Aleksandra , Carvalho Mariana R. , Culebras Mario , Cantarero Andres , Beaucamp Anne , Reis Rui L. , Oliveira J. Miguel , Collins Maurice N. 
Published: May 2023

AbstractPeripheral nerve injury (PNI) often clinically relies on the use of nerve grafts taken from the patient to establish a therapeutic effect, though secondary site of injury and morbidity have prompted the medical community to find alternative solutions. A new trend in the development of biomaterials arises in the form of electro-conductive biomaterials, especially for electrically active tissues such as the peripheral nerves. In this work, novel poly(3,4-ethylenedioxythiophene) PEDOT nanoparticles (PEDOT NPs) were synthetized via the mini-emulsion method and were combined with silk fibroin (SF) to create conduits for PNI repair. The synthesized PEDOT NPs-loaded SF conduits showed optimal properties for peripheral nerve substitution from the physico-chemical and biological point of view. They displayed excellent mechanical and conductivity performance with the tensile moduli reaching 6.61 ± 0.55 MPa and the conduits reaching 5.4 · 10–4 S cm−1, respectively. The conduits did not possess apatite-forming capacity, which were resistant to bending occlusions for angles up to 50° and to suturing. The developed conduits are promising as a novel biomaterial for applications in peripheral nerve regeneration; in vitro experiments showed that they did not allow BJ fibroblast infiltration, avoiding scar tissue formation in the lumen, and they did not show any toxic effect for Schwann cells.