bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronosequence of invasion reveals minimal losses of population genomic diversity, niche expansion, and trait divergence in the polyploid, leafy spurge
Authors: Lake Thomas A. , Briscoe Runquist Ryan D. , Flagel Lex E. , Moeller David A. 
Published: April 2023

ABSTRACTRapid evolution may play an important role in the range expansion of invasive species and modify forecasts of invasion, which are the backbone of land management strategies. However, losses of genetic variation associated with colonization bottlenecks may constrain trait and niche divergence at leading range edges, thereby impacting management decisions that anticipate future range expansion. The spatial and temporal scales over which adaptation contributes to invasion dynamics remains unresolved. We leveraged detailed records of the ∼130 year invasion history of the invasive polyploid plant, leafy spurge (Euphorbia virgata), across ∼500km in Minnesota, U.S.A. We examined the consequences of range expansion for population genomic diversity, niche breadth, and the evolution of germination behavior. Using genotyping-by-sequencing, we found some population structure in the range core, where introduction occurred, but panmixia among all other populations. Range expansion was accompanied by only modest losses in sequence diversity, with small, isolated populations at the leading edge harboring similar levels of diversity to those in the range core. The climatic niche expanded during most of range expansion, and the niche of the range core was largely non-overlapping with the invasion front. Ecological niche models indicated that mean temperature of the warmest quarter was the strongest determinant of habitat suitability and that populations at the leading edge had the lowest habitat suitability. Guided by these findings, we tested for rapid evolution in germination behavior over the time course of range expansion using a common garden experiment and temperature manipulations. Germination behavior diverged from early to late phases of the invasion, with populations from later phases having higher dormancy at lower temperatures. Our results suggest that trait evolution may have contributed to niche expansion during invasion and that distribution models, which inform future management planning, may underestimate invasion potential without accounting for evolution.
Performance comparison of high throughput single-cell RNA-Seq platforms in complex tissues
Authors: Colino-Sanguino Yolanda , de la Fuente Laura Rodriguez , Gloss Brian , Law Andrew M. K. , Handler Kristina , Pajic Marina , Salomon Robert , Gallego-Ortega David , Valdes-Mora Fatima 
Published: April 2023

AbstractSingle-cell transcriptomics has emerged as the preferred tool to define cell identity through the analysis of gene expression signatures. However, there are limited studies that have comprehensively compared the performance of different scRNAseq systems in complex tissues. Here, we present a systematic comparison of two well-established high throughput 3’-scRNAseq platforms: 10x Chromium and BD Rhapsody, using tumours that present high cell diversity. Our experimental design includes both fresh and artificially damaged samples from the same tumours, which also provides a comparable dataset to examine their performance under challenging conditions. The performance metrics used in this study consist of gene sensitivity, mitochondrial content, reproducibility, clustering capabilities, cell type representation and ambient RNA contamination. These analyses showed that BD Rhapsody and 10x Chromium have similar gene sensitivity, while BD Rhapsody has the highest mitochondrial content. Interestingly, we found cell type detection biases between platforms, including a lower proportion of endothelial and myofibroblast cells in BD Rhapsody and lower gene sensitivity in granulocytes for 10x Chromium. Moreover, the source of the ambient noise was different between plate-based and droplet-based platforms. In conclusion, our reported platform differential performance should be considered for the selection of the scRNAseq method during the study experimental designs.
Distinct roles ofBdnf IandBdnf IVtranscript variant expression in hippocampal neurons
Authors: Bach Svitlana V. , Bauman Allison J. , Hosein Darya , Tuscher Jennifer J. , Ianov Lara , Greathouse Kelsey M. , Henderson Benjamin W. , Herskowitz Jeremy H. , Martinowich Keri , Day Jeremy J. 
Published: April 2023

SummaryBrain-derived neurotrophic factor (Bdnf) plays a critical role in brain development, dendritic growth, synaptic plasticity, as well as learning and memory. The rodentBdnfgene contains nine 5′ non-coding exons (I-IXa), which are spliced to a common 3′ coding exon (IX). Transcription of individualBdnfvariants, which all encode the same BDNF protein, is initiated at unique promoters upstream of each non-coding exon, enabling precise spatiotemporal and activity-dependent regulation ofBdnfexpression. Although prior evidence suggests thatBdnftranscripts containing exonI(Bdnf I) or exonIV(Bdnf IV) are uniquely regulated by neuronal activity, the functional significance of differentBdnftranscript variants remains unclear. To investigate functional roles of activity-dependentBdnf IandIVtranscripts, we used a CRISPR activation (CRISPRa) system in which catalytically-dead Cas9 (dCas9) fused to a transcriptional activator (VPR) is targeted to individualBdnfpromoters with single guide RNAs (sgRNAs), resulting in transcript-specificBdnfupregulation.Bdnf Iupregulation is associated with gene expression changes linked to dendritic growth, whileBdnf IVupregulation is associated with genes that regulate protein catabolism. Upregulation ofBdnf I, but notBdnf IV, increased mushroom spine density, volume, length, and head diameter, and also produced more complex dendritic arbors in cultured rat hippocampal neurons. In contrast, upregulation ofBdnf IV, but notBdnf I, in the rat hippocampus attenuated contextual fear expression. Our data suggest that whileBdnf IandIVare both activity-dependent, BDNF produced from these promoters may serve unique cellular, synaptic, and behavioral functions.
“It's your fault!” – said a public client to modernity advocates: an exploration of UK public sector’s viewpoints on the modern methods of construction
Authors: Saad Ali M. , Zulu Sambo Lyson , Dulaimi Mohammed 
Published: April 2023

Purpose
The staggering demand for construction projects to meet a spectrum of public needs is projected to outstrip the industry’s supply capability. The modern methods of construction (MMC) offers wider control due to shifting key construction processes offsite. Public clients play a significant role due to their purchasing power; however, their uptake of MMC is low, despite the benefits. The purpose of this study is to reveal the reasoning behind such low adoption. The research gap, herewith, is our lack of understanding of the influence of public clients perceptions on their adoption’s indecision.

Design/methodology/approach
This study used a qualitative approach to investigate the motives behind the public sector’s low MMC adoption. Semi-structured interviews with 14 of the United Kingdom’s public sector decision-makers, industry leaders and experts have been conducted. Perspectives were argued against the diffusion of innovation (DOI) theory.

Findings
Overall, the innovation’s attributes informed the authors of the positive perceptions from the public sector, demonstrating that the low adoption of MMC is not linked to any embedded issues with the innovation itself rather being predominantly related to the dynamics between supply and demand. The former (supply), reflected a failure in communicating confidence, and the latter (demand), attained characteristics that are limiting wider uptake.

Originality/value
To the best of the authors’ knowledge, this is the first study to apply the DOI theory to reveal the relationship between UK public clients’ perceptions and their decision-making. Moreover, this paper addresses the scant attention to the use of theories to explain the flow of innovations in the construction context.
Simmelian ties for SMEs' green-innovation improvement in manufacturing industries: mediating and moderating effects
Authors: Cheng Xinsheng , Xu Yingjie , Li Fengshu 
Published: April 2023

PurposeThis study had a threefold aim: to examine the impact of a Simmelian-tie tripartite alliance on corporate green innovation; to determine the chain-mediating roles of knowledge acquisition and knowledge integration; and to identify the moderating effect of network routines on the relationship between a Simmelian tie and green innovation.Design/methodology/approachData were collected through 487 valid survey questionnaires from Chinese small and medium-sized manufacturing enterprises (SMEs). The authors examined the data through a structural model using partial least-squares structural-equation modeling (PLS-SEM) to test the research hypotheses.FindingsThe results reveal several key factors with positive impacts on enterprise green innovation. Specifically, a Simmelian tie significantly and positively affects enterprise green innovation. The results further reveal that knowledge acquisition and integration play mediating roles, while a network routine positively moderates the relationships among a Simmelian tie, knowledge acquisition and integration, and corporate green innovation.Originality/valueThis study is among the earliest empirical studies to investigate the influence of Simmelian ties on corporate green innovation for manufacturing companies. This study provides a theoretical basis for managers of firms, especially those of SMEs with limited resources, to fully use Simmelian ties to achieve environmentally sustainable innovation. In addition, this study validates and extends knowledge-management theory by verifying the linking roles of knowledge acquisition and integration and facilitating role of network routines.
Technology adaptation: micro new ventures in a COVID-19 lockdown
Authors: Felzensztein Christian , Tretiakov Alexei 
Published: April 2023

PurposeThere is a paucity of evidence on how small new ventures cope with shifts from physical space to cyberspace imposed by external crises, such as pandemics. Further, even though the concept of space is highly relevant to understanding entrepreneurship, the concept has been underutilised in entrepreneurship research. In particular, the potential of understanding entrepreneurship in terms of the interplay between physical space and cyberspace is yet to be explored. The authors address these research gaps by pursuing the following research question: How did micro new ventures experience the shift from physical space to cyberspace (technology adaptation) imposed by the coronavirus disease 2019 (COVID-19) crisis?Design/methodology/approachData were collected via semi-structured interviews with founders of start-ups associated with two incubators, in Spain and Monaco. Thematic analysis of interview transcripts was conducted, approaching the data with the focus on firm positioning in “real” space and in cyberspace and on possible transformations of business models.FindingsThe pandemic opened new opportunities for small new ventures, as many start-ups were successful in shifting into cyberspace by undergoing a radical digital transformation and ended up with more scalable business models and in many cases transformed themselves into micro-multinationals.Research limitations/implicationsOverall, firms tended to shift from physical space to cyberspace, following the firms' customers to cyberspace, finding new and more international, customers in cyberspace or guiding the firms' existing customer base into cyberspace. Firms that maintained the pre-pandemic position were either already fully digital or had sufficient resources to hold position in the anticipation of the post-pandemic future.Originality/valueThe authors introduce the concept of cyberspace in the context of entrepreneurship studies and explore the trajectories of firms in a crisis.
Tourism and public insecurity: what are its roots?
Authors: Coutinho Ana Catarina , Nóbrega Wilker Ricardo 
Published: April 2023

Purpose
Studies examining public insecurity and tourism tend to develop their research from the tourist's perspective, thereby focusing on its consequences, which insufficiently and poorly contribute to the development process. With that in mind, the purpose of this study is to discuss the root causes of public insecurity and the urban development process surrounding tourism dynamics in Brazil's northeast region.

Design/methodology/approach
The complexity method was used to collect both documentary and normative data to understand the reality beyond the data on accommodations and vertical real estate developments over time. These data were organized in ArcGIS (version 10.5) and are discussed within the context of urban development, public insecurity and tourism theories.

Findings
The results of this study demonstrate that the relationship between tourism and public insecurity is rooted in discussions surrounding the urban development process, remodeled by the physical and symbolic dimensions surrounding the production of space. Nevertheless, the symbolism of tourism is not a constant when it comes to socio-spatial changes but rather acts as a physical and secondary dynamic throughout the entire reconfiguration process.

Originality/value
The findings of this study indicate that this study can be used for the elaboration of security policies in tourist destinations in Latin America that present high levels of violence, considering the discourses at implementing urban legislation.
Factors leading to sustainable consumption behavior: an empirical investigation among millennial consumers
Authors: Ghaffar Abdul , Islam Tahir 
Published: April 2023

PurposePromoting sustainable consumption is key to global sustainable development. The current study aims to explore the impact of environmental risk perception, environmental knowledge, social pressure and health consciousness on sustainable behavior intention among millennial consumers.Design/methodology/approachThis study used a survey method and a self-administered questionnaire to obtain data from the millennial generation. Smart partial least square (PLS) 4.0 was used to analyze data. This research used judgmental sampling and collected 596 valid responses.FindingsThe present study indicates that sustainable consumption is becoming more prevalent among millennials. The authors observed that a high level of environmental risk perception, environmental knowledge, social pressure and health consciousness are the antecedents of sustainable behavioral intention, ultimately leading to sustainable consumption behavior.Research limitations/implicationsThe current study highlights the millennial generation's sustainable consumption behavior. Researchers, policymakers, scientists, managers, industry professionals and brand managers can use the research findings to establish a sustainable framework.Originality/valueThis research finds that promoting ecological consumption and environmental consciousness can help developing countries achieve environmental sustainability and ecological balance. The research findings add to the literature by offering new insights into customers' pricing perception for sustainable products and sustainable product availability toward sustainable consumption behavior in developing countries.
The power of presentation: How attire, cosmetics, and posture impact the source credibility of women expert witnesses
Authors: Jones Ashley C. T. , Repke Alexandra , Batastini Ashley B. , Sacco Donald , Dahlen Eric R. , Mohn Richard S. 
Published: April 2023

AbstractGender stereotypes may negatively affect perceptions of women professionals' credibility, including forensic experts. This study investigated the impact of behavior‐based and appearance‐based factors on women expert witness's credibility. Jury‐eligible adults were shown one of 16 conditions depicting a woman expert which varied based on combinations of three primary independent variables: (1) attire, (2) cosmetic use, and (3) posture. Expert attractiveness and participants' sexist attitudes served as covariates. Results revealed that women experts were seen as marginally more credible when wearing a skirt suit with a closed posture stance than when wearing a pant suit with a closed posture. Secondary analyses indicated expert attractiveness and participant sexist attitudes accounted for the most variability in credibility scores. Credibility of women expert witnesses may be impacted by irrelevant peripheral cues. Findings can inform discussions aimed at mitigating extraneous factors that inadvertently undermine the reception of women expert witness testimony.
Factors behind the threshold‐like changes in lake ecosystems along a water colour gradient: The effects of dissolved organic carbon and iron on euphotic depth, mixing depth and phytoplankton biomass
Authors: Horppila Jukka , Keskinen Saana , Nurmesniemi Miia , Nurminen Leena , Pippingsköld Ella , Rajala Salla , Sainio Kari , Estlander Satu 
Published: April 2023

Abstract

The effects of water colour, dissolved organic carbon (DOC), iron (Fe) and fetch on the mixing depth (Zmix), euphotic depth (Zeu) and phytoplankton biomass of lakes were studied to clarify the factors behind changes in lake productivity along a DOC gradient.

The study was conducted by field sampling 102 lakes in Finland. The lakes were situated mainly in forested areas.

At DOC concentrations below 15 mg/L, light attenuation was explained both by Fe and DOC, but at higher DOC concentrations the effect of Fe faded. For the absorption of different wavelengths of photosynthetic light, both DOC and Fe had a significant effect up to 475 nm, but for longer wavelengths only the effect of DOC was significant.

Both Zmix and Zeu decreased logarithmically with increasing DOC and water colour. Water colour was determined by DOC and Fe. Zmix increased along with increasing fetch. Zmix exceeded Zeu when DOC concentration was >14.4 mg/L and water colour >106 mg Pt/L (platinum as chloroplatinate ion 3).

Above the 14.4 mg/L DOC threshold, the illuminated part of the mixed layer decreased logarithmically with increasing water colour, leaving an increasing part of the mixed epilimnion below the photosynthetic layer. Chlorophyll‐a concentration showed a unimodal relationship with DOC, with the highest biomass occurring at DOC concentrations of 15.7–15.9 mg/L.

The results suggested that above DOC concentration 14 mg/L and water colour 106 mg Pt/L, both quality and quantity of light in the epilimnion restrict production by phytoplankton. This can explain the observed threshold‐like changes in lake productivity along a DOC gradient.
Toward developing care outcome quality indicators for home care for older people: A prospective cohort study in Japan
Authors: Eltaybani Sameh , Kitamura Satomi , Fukui Chie , Igarashi Ayumi , Sakka Mariko , Noguchi‐Watanabe Maiko , Takaoka Manami , Inagaki Asa , Yasaka Taisuke , Kobayashi Hiromi , Yamamoto‐Mitani Noriko 
Published: April 2023

IntroductionCare quality in Japan's long‐term care (LTC) agencies, including home care, is the responsibility primarily of individual agencies, and the evaluation of service processes and outcomes is minimal.ObjectivesTo describe the development of quality indicators for LTC (QIs‐LTC) in Japan.MethodsQIs‐LTC were developed through literature review and expert panel discussions and then were piloted and used in a 2‐year longitudinal survey. The survey (launched in September 2019) targeted older people receiving home care (n = 1450), their family members (n = 880), their professional home care providers (n = 577), and managers of home care agencies (n = 122).ResultsAcross eight domains (maintaining dignity, minimizing symptoms and disease deterioration, maintaining nutritional status, maintaining bladder/bowel control, encouraging physical activities, experiencing sound sleep, maintaining serenity and contentedness, and maintaining family's well‐being), 24 care quality objectives were set with 24 outcome QIs‐LTC and 144 process QIs‐LTC. In the survey, 84.8% of clients were using home care nursing, 26.3% were living alone, and 39.5% had dementia. In the month preceding the data collection, 13.9% of clients had a new disease or worsening of an existing disease, 8.8% were hospitalized at least once, and 47.9% did not participate in activities of interest. About 20% of clients' families were unable to spend time peacefully, and 52.8% were exhausted from the client's care.ConclusionsThe QIs‐LTC developed in the current study are generic and client‐ and family‐centered. They encompass objective and subjective information and would facilitate standardized monitoring if adopted and comparison between LTC settings, including home care. In addition, future research directives are outlined. Geriatr Gerontol Int 2023; 23: 383–394.
Amplifying invisibility: COVID‐19 and Zimbabwean migrant farm workers in South Africa
Authors: Addison Lincoln 
Published: April 2023

AbstractHow does the COVID‐19 pandemic impact migrant worker visibility? This paper examines how the pandemic underscores the invisibility of Zimbabwean migrant farm workers employed at ZZ2, one of the largest commercial farms in South Africa. I argue that Zimbabweans are made invisible in three ways. First, employer and state restrictions on mobility, alongside rising xenophobia in South Africa, leave migrant workers hyper‐visible to ZZ2 management, yet invisible to most people outside the farm. Second, ZZ2 avoids discussion of its migrant workforce in public forums, even as it faces increased scrutiny for its treatment of its workers during the pandemic. Third, the most prominent critic of ZZ2—the Economic Freedom Fighters (EFF)—grants migrant workers only a partial visibility as undifferentiated foreigners with no voice, a construction that ultimately maintains their invisibility at the company. Taken together, these interlocking forms of invisibilization diminish the structural and associational power of workers.