bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of serum miRNAs expression in frail and robust subjects undergoing multicomponent exercise protocol (VIVIFRAIL)
Authors: Agostini Simone , Mancuso Roberta , Citterio Lorenzo Agostino , Mihali Gabriela Alexandra , Arosio Beatrice , Clerici Mario 
Published: February 2023

Abstract
Background
Frailty, defined as physical performance impairment, is a common condition in older adults and can anticipate the development of sarcopenia, a geriatric syndrome characterized by loss of muscle strength and mass. microRNAs (miRNAs) are short molecules of RNA endowed with the ability to modulate gene expression; miRNAs are present in serum and are considered potential biomarkers for several diseases. Serum concentration of miR-451a, miR-93-5p, miR-155-5p, miR-421-3p, miR-425-5p, miR-495-3p and miR-744-5p was recently shown to be altered in sarcopenic patients.

Methods
We verified if a particular miRNAs pattern could be detected in frailty as well by analyzing these molecules in 50 frail and 136 robust subjects. Additionally, a subgroup of these subjects (15 frail and 30 robust) underwent a 12-week program based on a multicomponent exercise protocol (VIVIFRAIL) consisting of resistance training, gait retraining, and balance training. After the program, serum miRNAs concentration was measured again, to verify whether the physical activity had an effect on their concentration. Moreover, clinical characteristics and indicators of physical performance of all subjects were compared before and after intervention to verify the effect of the VIVIFRAIL program.

Results
At the end of the multicomponent exercise program, Short Physical Performance Battery (SPPB) score as well right and left handgrip (p < 0.05) were significantly increased in frail subjects; right and left handgrip significantly were increased also in robust subjects (p < 0.05). Interestingly, the variation of SPPB was significantly higher in frail compared to robust subjects (p < 0.0001). Moreover, at the end of the program, in frail compared to robust subjects: miR-451a serum concentration was significantly increased (frail: 6.59 × 104; 1.12 × 104–2.5 × 105 c/ng; robust: 2.31 × 104; 1.94 × 103–2.01 × 105 c/ng) (p < 0.05); and 2) miR-93-5p and miR-495-3p serum concentration was reduced, whereas that of miR-155-5p was significantly increased (p < 0.05 in both cases). Serum concentration of miR-93-5p and miR-495-3p was decreased, and that of miR-155-5p was increased at the end of the program in robust subjects alone, statistical significance being reached for miR-93-5p alone (p = 0.02).

Conclusion
These results suggest that serum miR-451a should be investigated as a potential biomarker for frailty and show that the VIVIFRAIL multicomponent program modulates circulatory miRNAs expression, at least in older adults.
Proteomic analysis predicts anti-angiogenic resistance in recurred glioblastoma
Authors: Jeon Hanwool , Byun Joonho , Kang Hayeong , Kim Kyunggon , Lee Eunyeup , Kim Jeong Hoon , Hong Chang Ki , Song Sang Woo , Kim Young-Hoon , Chong Sangjoon , Kim Jae Hyun , Nam Soo Jeong , Park Ji Eun , Lee Seungjoo 
Published: February 2023

Abstract
Background
Recurrence is common in glioblastoma multiforme (GBM) because of the infiltrative, residual cells in the tumor margin. Standard therapy for GBM consists of surgical resection followed by chemotherapy and radiotherapy, but the median survival of GBM patients remains poor (~ 1.5 years). For recurrent GBM, anti-angiogenic treatment is one of the common treatment approaches. However, current anti-angiogenic treatment modalities are not satisfactory because of the resistance to anti-angiogenic agents in some patients. Therefore, we sought to identify novel prognostic biomarkers that can predict the therapeutic response to anti-angiogenic agents in patients with recurrent glioblastoma.

Methods
We selected patients with recurrent GBM who were treated with anti-angiogenic agents and classified them into responders and non-responders to anti-angiogenic therapy. Then, we performed proteomic analysis using liquid-chromatography mass spectrometry (LC–MS) with formalin-fixed paraffin-embedded (FFPE) tissues obtained from surgical specimens. We conducted a gene-ontology (GO) analysis based on protein abundance in the responder and non-responder groups. Based on the LC–MS and GO analysis results, we identified potential predictive biomarkers for anti-angiogenic therapy and validated them in recurrent glioblastoma patients.

Results
In the mass spectrometry-based approach, 4957 unique proteins were quantified with high confidence across clinical parameters. Unsupervised clustering analysis highlighted distinct proteomic patterns (n = 269 proteins) between responders and non-responders. The GO term enrichment analysis revealed a cluster of genes related to immune cell-related pathways (e.g., TMEM173, FADD, CD99) in the responder group, whereas the non-responder group had a high expression of genes related to nuclear replisome (POLD) and damaged DNA binding (ERCC2). Immunohistochemistry of these biomarkers showed that the expression levels of TMEM173 and FADD were significantly associated with the overall survival and progression-free survival of patients with recurrent GBM.

Conclusions
The candidate biomarkers identified in our protein analysis may be useful for predicting the clinical response to anti-angiogenic agents in patients with recurred GBM.
Quantification of the three-dimensional root system architecture using an automated rotating imaging system
Authors: Wu Qian , Wu Jie , Hu Pengcheng , Zhang Weixin , Ma Yuntao , Yu Kun , Guo Yan , Cao Jing , Li Huayong , Li Baiming , Yao Yuyang , Cao Hongxin , Zhang Wenyu 
Published: February 2023

Abstract
Background
Crop breeding based on root system architecture (RSA) optimization is an essential factor for improving crop production in developing countries. Identification, evaluation, and selection of root traits of soil-grown crops require innovations that enable high-throughput and accurate quantification of three-dimensional (3D) RSA of crops over developmental time.

Results
We proposed an automated imaging system and 3D imaging data processing pipeline to quantify the 3D RSA of soil-grown individual plants across seedlings to the mature stage. A multi-view automated imaging system composed of a rotary table and an imaging arm with 12 cameras mounted with a combination of fan-shaped and vertical distribution was developed to obtain 3D image data of roots grown on a customized root support mesh. A 3D imaging data processing pipeline was developed to quantify the 3D RSA based on the point cloud generated from multi-view images. The global architecture of root systems can be quantified automatically. Detailed analysis of the reconstructed 3D root model also allowed us to investigate the Spatio-temporal distribution of roots. A method combining horizontal slicing and iterative erosion and dilation was developed to automatically segment different root types, and identify local root traits (e.g., length, diameter of the main root, and length, diameter, initial angle, and the number of nodal roots or lateral roots). One maize (Zea mays L.) cultivar and two rapeseed (Brassica napus L.) cultivars at different growth stages were selected to test the performance of the automated imaging system and 3D imaging data processing pipeline.

Conclusions
The results demonstrated the capabilities of the proposed imaging and analytical system for high-throughput phenotyping of root traits for both monocotyledons and dicotyledons across growth stages. The proposed system offers a potential tool to further explore the 3D RSA for improving root traits and agronomic qualities of crops.
Recurrence rate of intervertebral disc disease in surgically treated French Bulldogs: a retrospective study (2009–2019)
Authors: Leu Dominique , Vidondo Beatriz , Stein Veronika , Forterre Franck 
Published: February 2023

AbstractBackgroundIntervertebral disc disease (IVDD) is a common diagnosis and well-investigated pathological condition in French Bulldogs with neurological deficiencies. However there is currently only one recently published retrospective descriptive study looking for recurrence rates of IVDD in French Bulldogs. Medical reports of French Bulldogs with a first episode of IVDD and surgical treatment were evaluated and reviewed for clinical signs of recurrence. Risk factors for Total-Recurrence, Cervical- and Thoracolumbar-Recurrence were evaluated by means of logistic regression models. The aim of this study was to assess frequency and risk factors associated with the recurrence of IVDD in French Bulldogs.ResultsOne hundred twenty-seven French Bulldogs with a first episode of IVDD and surgical treatment were evaluated. 52.7% (67/127) of these patients showed signs of recurrence. The recurrence rate in the cervical spine was slightly lower (47%) compared to the thoracolumbar spine (56.6%). A significant association with recurrence could be found for the factor age: French Bulldogs with a first episode of IVDD ≤ 3 years seem to be prone for Total-Recurrence (P = 0.002) and Cervical-Recurrence (with ORs ranging from 0.02 to 0.03 for patients older than 3 years). 50% of the recurrences (median) occurred within the first 12 month after the first episode of IVDD.ConclusionsRecurrence of IVDD can be expected in more than half of French Bulldogs affected by IVDD. Especially young French Bulldogs are prone for recurrence in cervical spine. Almost every fourth patient with IVDD suffers from a recurrence within 12 months. Future dog owners should be informed about the risk of IVDD and the early onset of recurrences in French Bulldogs.
Lateral compression type 1 fracture fixation in the elderly (L1FE): study protocol for a randomised controlled trial (with internal pilot) comparing the effects of INFIX surgery and non-surgical management for treating patients with lateral compression type 1 (LC-1) fragility fractures
Authors: Cook Elizabeth , Laycock Joanne , Acharya Mehool , Backhouse Michael Ross , Corbacho Belen , Doherty Laura , Forward Daren , Hewitt Catherine , Hilton Catherine , Hull Peter , Kassam Jamila , Maturana Camila , Mcdaid Catriona , Roche Jenny , Sivapathasuntharam Dhanupriya , Torgerson David , Bates Peter 
Published: February 2023

Abstract
Background
Lateral compression type1 (LC-1) fragility fractures are a common, painful injury in older adults resulting in reduced mobility. The incidence of these fractures is increasing with the growing older adult population. The current standard of care is non-surgical management; however, patients with this injury are at risk of long-term immobility and related complications. INFIX is a pelvic fixation device used in younger patients with high-energy fractures. The device is fitted via a percutaneous technique with no external pin sites and has good purchase even in osteoporotic bone. It therefore has the potential to be well tolerated in patients with LC-1 fragility fractures. INFIX could improve patients’ ability to mobilise and reduce the risk of immobility-related complications. However, there is a risk of complications related to surgery, and robust evidence is required on patient outcomes. This study will investigate the clinical and cost-effectiveness of surgical fixation with INFIX compared to non-surgical management of LC-1 fragility fractures in older adults.

Methods
A multi-centre randomised controlled trial of 600 patients allocated 1:1 to non-surgical management or INFIX surgery. The study will have a 12-month internal pilot to assess recruitment and trial feasibility. The primary outcome will be the patient quality of life over 6 months, measured by the patient-reported EQ-5D-5L. The secondary outcomes will include physical function, mental health, pain, delirium, imaging assessment, resource use, and complications.

Discussion
The L1FE study aims to compare the clinical and cost-effectiveness of surgical and non-surgical management of people aged 60 years and older with LC-1 fragility fractures. The trial is sufficiently powered and rigorously designed to inform future clinical and patient decision-making and allocation of NHS resources.

Trial registration
International Standard Randomised Controlled Trial Number Registry ISRCTN16478561. Registered on 8 April 2019
Draft genomes of Blastocystis subtypes from human samples of Colombia
Authors: Higuera Adriana , Salas-Leiva Dayana E. , Curtis Bruce , Patiño Luz H. , Zhao Dandan , Jerlström-Hultqvist Jon , Dlutek Marlena , Muñoz Marina , Roger Andrew J. , Ramírez Juan David 
Published: February 2023

Abstract
Background
Blastocystis is one of the most common eukaryotic microorganisms colonizing the intestines of both humans and animals, but the conditions under which it may be a pathogen are unclear.

Methods
To study the genomic characteristics of circulating subtypes (ST) in Colombia, we established nine xenic cultures from Blastocystis isolated from human fecal samples, we identified 10 different subtypes, since one sample had a mixed infection. Thus, the genomes of the subtypes ST1 (n = 3), ST2 (n = 1), ST3 (n = 2), ST6 (n = 1), ST7 (n = 1), and ST8 (n = 2) were sequenced using Illumina and Oxford Nanopore Technologies (ONT).

Results
Analyses of these draft nuclear genomes indicated remarkable diversity in terms of genome size and guanine-cytosine (GC) content among the compared STs. Illumina sequencing-only draft genomes contained 824 to 2077 scaffolds, with total genome size ranging from 12 to 13.2 Mb and N50 values ranging from 10,585 to 29,404 base pairs (bp). The genome of one ST1 isolate was sequenced using ONT. This assembly was more contiguous, with a size of 20 million base pairs (Mb) spread over 116 scaffolds, and an N50 of 248,997 bp.

Conclusion
This work represents one of the few large-scale comparative genomic analyses of Blastocystis isolates, providing an additional glimpse into its genomic diversity.

Graphical Abstract
“Role of radiology in a multidisciplinary approach to patient care”: summary of the ESR International Forum 2022
Authors:   European Society of Radiology (ESR)
Published: February 2023

AbstractAt the ESR International Forum 2022, held at ECR 2022 (July 16 in Vienna, Austria) different views, initiatives, and ideas from participating societies were presented with regard to the position and role of radiology in the changing landscape of health care and its role as an integral part of multidisciplinary teams. While it is unanimously agreed that multidisciplinarity is a key aspect of modern patient care, it creates new challenges that need to be addressed.
Monkeys do not show sex differences in toy preferences through their individual choices
Authors: Pittet Florent , Heng Victoria , Atufa Jala , Bliss-Moreau Eliza 
Published: February 2023

Abstract
Background
As interest in evaluating sex differences in nonhuman animals grows, the finding that male and female monkeys have toy preferences that differ, and that parallel those documented in human children, has garnered significant attention and is leveraged as an argument in favor of a biological contribution for human sex differences. To date, however, only two studies have investigated sex differences in monkeys’ toy preferences, both documenting that males prefer toys considered to be “masculine” (such as vehicles) and females prefer toys considered to be “feminine” (such as dolls). Monkeys in these studies were tested in their social groups, making it hard to determine if the sex differences reported reflect actual individual preferences or result from social dynamics present at the time of testing.

Method
Here, we assessed the preferences of 14 rhesus macaques (N = 7 males; N = 7 females) who were singly tested in a choice test with a variety of toys characterized as masculine (hard non-zoomorphic wheeled toys), feminine (zoomorphic soft toys), neutral (hard non-zoomorphic toys) and ambiguous (zoomorphic or plush vehicles) based on criteria from previous studies.

Results
Males and females showed similar preferences for neutral and “masculine” toys and preferred them (i.e., were more likely to interact with them) to “feminine” and sex-ambiguous toys. When they interacted with the toys, both males and females interacted more with neutral than with “masculine” toys. Females, but not males, interacted more with neutral and “masculine” toys than with “feminine” toys. The highest frequency of interaction for any single toy for the male monkeys was with the doll—standing is stark contrast to previous findings.

Conclusions
Our results contrast greatly with the previous study in rhesus monkeys, as well as findings in human children, suggesting that the previously documented sex differences are likely context dependent, and question the existence of a strong biological basis to sex differences in toy preferences.
RNA binding protein SAMD4: current knowledge and future perspectives
Authors: Wang Xin-Ya , Zhang Li-Na 
Published: February 2023

AbstractSAMD4 protein family is a class of novel RNA-binding proteins that can mediate post-transcriptional regulation and translation repression in eukaryotes, which are highly conserved from yeast to humans during evolution. In mammalian cells, SAMD4 protein family consists of two members including SAMD4A/Smaug1 and SAMD4B/Smaug2, both of which contain common SAM domain that can specifically bind to different target mRNAs through stem-loop structures, also known as Smaug recognition elements (SREs), and regulate the mRNA stability, degradation and translation. In addition, SAMD4 can form the cytoplasmic mRNA silencing foci and regulate the translation of SRE-containing mRNAs in neurons. SAMD4 also can form the cytosolic membrane-less organelles (MLOs), termed as Smaug1 bodies, and regulate mitochondrial function. Importantly, many studies have identified that SAMD4 family members are involved in various pathological processes including myopathy, bone development, neural development, and cancer occurrence and progression. In this review, we mainly summarize the structural characteristics, biological functions and molecular regulatory mechanisms of SAMD4 protein family members, which will provide a basis for further research and clinical application of SAMD4 protein family.
Sacrococcygeal epidural administration of 0.5% bupivacaine in seven cats undergoing pelvic or hind limb orthopaedic procedures
Authors: Torruella Xavier , Potter Joanna , Huuskonen Vilhelmiina 
Published: February 2023

Abstract
Background
Epidural administration of local anaesthetic agents provides good intraoperative antinociception for orthopaedic procedures of the pelvis and the pelvic limb. However, in cats the spinal cord extends approximately to the level of the first sacrococcygeal vertebra, and therefore the sacrococcygeal epidural could be a safer alternative to the lumbosacral epidural in cats. This case series describes perioperative analgesia and the haemodynamic status of seven client-owned cats that received sacrococcygeal epidural injection of 0.5% bupivacaine and underwent orthopaedic hind leg or pelvic surgeries under general anaesthesia.

Case presentation
Each cat received either 0.2 or 0.3 mL/kg of 0.5% bupivacaine with or without 0.2 mg/kg of morphine in the sacrococcygeal epidural space. Intraoperative antinociceptive response to surgical stimulus and haemodynamic changes were monitored and reported.

Conclusion
In these seven anaesthetised cats, 0.2 or 0.3 mL/kg of 0.5% bupivacaine, administered alone or in combination with morphine into the sacrococcygeal epidural space, enhanced antinociception so that intraoperative rescue analgesia was unnecessary in all but one cat. It also reduced the anticipated requirement for postoperative opioid use. However, a high incidence of hypotension was observed in the cats in this report, and hence intraoperative blood pressure monitoring should be considered mandatory in anaesthetised cats following epidural injection of local anaesthetic agents, regardless of injection site.
ISAC system assisted by RIS with sparse active elements
Authors: Asif Haider Mirza , Zhang Yimin D. , Aboutanios Elias 
Published: February 2023

AbstractIn this paper, we consider an integrated sensing and communications system assisted by a reconfigurable intelligent surface (RIS). A small number of sparsely placed active sensors are applied in the RIS to perform effective channels and, thereby, enable optimized beamforming for both communications and sensing objectives, namely, establishing reliable communication links with communication users (CUs) and effectively localize targets. The time-varying multipath channels between the RIS and the CU as well as the time-varying channel between the RIS and the targets are estimated by exploiting an interpolated Hermitian and Toeplitz covariance matrix followed by direction-of-arrival estimation using the MUSIC algorithm. Based on such results, we jointly optimize the transmit beamformer at the base station and the unit-modulus RIS passive beamformer. The RIS beamformer is optimized to maximize its minimum beam-pattern gain towards the desired sensing angles subject to the minimum signal-to-noise ratio requirement at the CU. Simulation results verify the effectiveness of the proposed approach, and the performance of different sparse array configurations is compared.
Supporting crime script analyses of scams with natural language processing
Authors: Lwin Tun Zeya , Birks Daniel 
Published: February 2023

AbstractIn recent years, internet connectivity and the ubiquitous use of digital devices have afforded a landscape of expanding opportunity for the proliferation of scams involving attempts to deceive individuals into giving away money or personal information. The impacts of these schemes on victims have shown to encompass social, psychological, emotional and economic harms. Consequently, there is a strong rationale to enhance our understanding of scams in order to devise ways in which they can be disrupted. One way to do so is through crime scripting, an analytical approach which seeks to characterise processes underpinning crime events. In this paper, we explore how Natural Language Processing (NLP) methods might be applied to support crime script analyses, in particular to extract insights into crime event sequences from large quantities of unstructured textual data in a scalable and efficient manner. To illustrate this, we apply NLP methods to a public dataset of victims’ stories of scams perpetrated in Singapore. We first explore approaches to automatically isolate scams with similar modus operandi using two distinct similarity measures. Subsequently, we use Term Frequency-Inverse Document Frequency (TF-IDF) to extract key terms in scam stories, which are then used to identify a temporal ordering of actions in ways that seek to characterise how a particular scam operates. Finally, by means of a case study, we demonstrate how the proposed methods are capable of leveraging the collective wisdom of multiple similar reports to identify a consensus in terms of likely crime event sequences, illustrating how NLP may in the future enable crime preventers to better harness unstructured free text data to better understand crime problems.