bluuebunny/crossref_metadata_2025_split huggingface dataset:
Baseline quality of life and chemotherapy toxicities in patients with early breast cancer
Authors: Licaj Idlir , Coquan Elodie , Dabakuyo‐Yonli Tienhan Sandrine , Dauchy Sarah , Vaz Luis Ines , Charles Cecile , Lemogne Cedric , Tredan Olivier , Vanlemmens Laurence , Jouannaud Christelle , Levy Christelle , Rigal Olivier , Fournier Marion , Petit Thierry , Dalenc Florence , Rouanet Philippe , Lemonnier Jerome , Everhard Sibille , Cottu Paul , Joly Florence 
Published: January 2023

AbstractBackgroundThe authors used the French breast cancer Cancer and Toxicities (CANTO) cohort to study the associations between baseline quality of life and chemotherapy dose‐reductions (CDRs) or postchemotherapy‐toxicities (PCTs).MethodsIn total, 3079 patients with breast cancer who received chemotherapy were included in this analysis. The associations between baseline physical functioning (PF) and fatigue measured using the European Organization for Research and Treatment of Cancer Quality‐of‐Life Questionnaire Core 30, and two endpoints—CDRs during adjuvant or neoadjuvant chemotherapy; and selected PCTs were estimated with odds ratios (ORs) and the corresponding 95% confidence intervals (CIs) using logistic regression models.ResultsAmong the 3079 patients from the CANTO cohort who were included, 718 (33.0%) received chemotherapy in the neoadjuvant setting, and 2361 (67.0%) received chemotherapy as adjuvant treatment. The chemotherapy included taxanes in 94.2% of patients and anthracyclines in 90.5% of patients. Overall, 15.5% of patients experienced CDRs and, 31.0% developed PCTs. Women with low baseline PF scores (<83) had higher multivariate odds of developing CDRs compared with those who had PF scores ≥83 (OR, 1.54; 95% CI, 1.13–2.09). The corresponding OR for PCTs was 1.50 (95% CI, 1.13–2.00). Women with high baseline fatigue scores had higher odds of CDRs (OR, 1.43; 95% CI, 1.13–1.76) and PCTs (OR, 1.32; 95% CI, 1.10–1.59).ConclusionsBy using the national CANTO cohort, baseline PF and fatigue were independently associated with CDRs and PCTs.
Discovery of 4‐(4‐aminophenyl)‐6‐phenylisoxazolo[3,4‐b]pyridine‐3‐amine derivatives as novel FLT3 covalent inhibitors for the intervention of acute myeloid leukemia
Authors: Wang Qing‐Xin , Wang Yi‐Bo , Sha Jiu‐Kai , Zhou Hai , Liu Jia‐Chuan , Wu Jia‐Zhen , Tong Zhen‐Jiang , Cai Jiao , Chen Zi‐Jun , Zhang Chen‐Qian , Zheng Xin‐Rui , Wang Jing‐Jing , Wang Xiao‐Long , Xue Xin , Yu Yan‐Cheng , Ding Ning , Leng Xue‐Jiao , Dai Wei‐Chen , Sun Shan‐Liang , Chang Liang , Li Nian‐Guang , Shi Zhi‐Hao 
Published: January 2023

AbstractSmall molecule covalent drugs have proved to be desirable therapies especially on drug resistance related to point mutations. Secondary mutations of FLT3 have become the main mechanism of FLT3 inhibitors resistance which further causes the failure of treatment. Herein, a series of 4‐(4‐aminophenyl)‐6‐phenylisoxazolo[3,4‐b]pyridine‐3‐amine covalent derivatives were synthesized and optimized to overcome the common secondary resistance mutations of FLT3. Among these derivatives, compound F15 displayed potent inhibition activities against FLT3 (IC50 = 123 nM) and FLT3‐internal tandem duplication (ITD) by 80% and 26.06%, respectively, at the concentration of 1 μM. Besides, F15 exhibited potent activity against FLT3‐dependent human acute myeloid leukemia (AML) cell lines MOLM‐13 (IC50 = 253 nM) and MV4‐11 (IC50 = 91 nM), as well as BaF3 cells with variety of secondary mutations. Furthermore, cellular mechanism assays indicated that F15 inhibited phosphorylation of FLT3 and its downstream signaling factors. Notably, F15 could be considered for further development as potential drug candidate to treat AML.
Western blot normalization: Time to choose a proper loading control seriously
Authors: Wang Qinhong , Han Wenqiang , Ma Chuanzhen , Wang Tianyu , Zhong Jingquan 
Published: January 2023

AbstractRecent research has questioned the validity of housekeeping proteins in Western blot. Our present study proposed new ideas for Western blot normalization that improved the reproducibility of scientific research. We used the Gene Expression Omnibus (GEO) database and the web tool GEO2R to exclude unstable housekeeping genes quickly. In ischemic heart tissues, actin and tubulin changed significantly, whereas no statistically significant changes were observed in the expression of genes relative to glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH). Besides, the reliability of GAPDH was further examined by Western blot. Additionally, unstable housekeeping genes were found in other animal models of cardiovascular medicine. We also found that sodium dodecyl sulfate and temperature significantly impacted the results of Ponceau S staining. Membranes stained with Ponceau S after immunodetection could avoid this interference, and the coefficients of variation for post‐immunodetection staining are lower than those produced by GAPDH immunodetection. Overall, we described a new use of differential gene expression analysis and proposed a modified Ponceau S staining method, which provided researchers with a proper loading control for Western blot and hence could improve reproducibility in research.
Quantitative interpretation of ToxTracker dose–response data for potency comparisons and mode‐of‐action determination
Authors: Boisvert Lorrie , Derr Remco , Osterlund Torben , Hendriks Giel , Brandsma Inger 
Published: January 2023

AbstractToxTracker is an in vitro mammalian stem cell‐based reporter assay that detects activation of specific cellular signaling pathways (DNA damage, oxidative stress, and/or protein damage) upon chemical exposure using flow cytometry. Here we used quantitative methods to empirically analyze historical control data, and dose–response data across a wide range of reference chemicals. First, we analyzed historical control data to define a fold‐change threshold for identification of a significant positive response. Next, we used the benchmark dose (BMD) combined‐covariate approach for potency ranking of a set of more than 120 compounds; the BMD values were used for comparative identification of the most potent inducers of each reporter. Lastly, we used principal component analysis (PCA) to investigate functional and statistical relationships between the ToxTracker reporters. The PCA results, based on the BMD results for all substances, indicated that the DNA damage (Rtkn, Bscl2) and p53 (Btg2) reporters are functionally complementary and indicative of genotoxic stress. The oxidative stress (Srxn1 and Blvrb) and protein stress (Ddit3) reporters are independent indicators of cellular stress, and essential for toxicological profiling using the ToxTracker assay. Overall, dose–response modeling of multivariate ToxTracker data can be used for potency ranking and mode‐of‐action determination. In the future, IVIVE (in vitro to in vivo extrapolation) methods can be employed to determine in vivo AED (administered equivalent dose) values that can in turn be used for human health risk assessment.
Mid‐height seismic isolation of equipment in nuclear power plants
Authors: Lal Kaivalya M. , Whittaker Andrew S. , Constantinou Michael C. 
Published: January 2023

AbstractAn innovative seismic isolation solution for designers of safety‐class equipment in advanced nuclear power plants is introduced. The test specimen was a tall, slender, carbon steel vessel that could represent a reactor vessel, steam generator, or a heat exchanger in a nuclear power plant: 240 inches tall, outer diameter of 60 inches, and wall thickness of 1 inch. The vessel was supported by three radial mounts at its mid‐height, near its center of gravity, on a steel frame. The vessel was subjected to three‐component ground motions using a 6DOF earthquake simulator. The specimen was filled with water for testing to indirectly account for the fluid and internal equipment present inside a prototype vessel. Three configurations were tested: non‐isolated, isolated using single Friction Pendulum (SFP) bearings, and isolated using triple Friction Pendulum (TFP) bearings. The test results demonstrate that mid‐height seismic isolation is practical and enables a significant reduction in horizontal spectral accelerations. These outcomes are not specific to the spherical sliding bearings used in the experiments but are broadly applicable to mid‐height, seismically isolated equipment.
DUSP12 ameliorates myocardial ischemia–reperfusion injury through HSPB8‐induced mitophagy
Authors: Cheng Jing , Ji Meihua , Jing Haijuan , Lin Hongqi 
Published: January 2023

AbstractThis study aimed to explore the role of dual specificity phosphatase 12 (DUSP12) in regulating myocardial ischemia–reperfusion (I/R) injury and the underlying mechanism. The expression of DUSP12 in myocardial tissues and heat‐shock protein beta‐8 (HSPB8) and mitophagy‐related proteins in myocardial tissues and H9c2 cells were detected by western blot analysis. The serum creatine kinase isoenzymes (CK‐MB) and lactate dehydrogenase (LDH), levels of reactive oxygen species and malondialdehyde, superoxide dismutase activity in myocardial tissues and H9c2 cells, and caspase‐3 activity in H9c2 cells were analyzed by corresponding assay kits. The infarct area in the rat's heart was observed by triphenyl tetrazolium chloride staining. The apoptosis of myocardial cells in myocardial tissues and H9c2 cells was detected by terminal‐deoxynucleotidyl transferase dUTP‐biotin nick‐end labeling assay. The interaction between DUSP12 and HSPB8 was clarified by the coimmunoprecipitation assay. The transfection efficacy of si‐HSPB8#1 and si‐HSPB8#2 in H9c2 cells was confirmed by real‐time quantitative‐polymerase chain reaction and western blot analysis. As a result, DUSP12 expression was downregulated in I/R rats, which was promoted by lentivirus‐expressing DUSP12. DUSP12 overexpression reduced the serum creatine kinase isoenzymes (CK‐MB) and LDH, decreased the infarct area in the rat's heart, and suppressed the apoptosis and oxidative stress in myocardial tissues. DUSP12 overexpression also upregulated the expression of HSPB8 to promote mitophagy. The coimmunoprecipitation assay indicated that DUSP12 could be combined with HSPB8. In addition, DUSP12 overexpression could inhibit hypoxia/reoxygenation‐elicited apoptosis as well as oxidative stress in H9c2 cells by upregulating HSPB8 expression to promote mitophagy, which was countervailed by HSPB8 deficiency. In conclusion, DUSP12 overexpression decreased the apoptosis and oxidative stress in myocardial I/R injury through HSPB8‐induced mitophagy.
Bacterial outer membrane vesicles induce a transcriptional shift in arabidopsis towards immune system activation leading to suppression of pathogen growth in planta
Authors: Chalupowicz Laura , Mordukhovich Gideon , Assoline Nofar , Katsir Leron , Sela Noa , Bahar Ofir 
Published: January 2023

AbstractGram‐negative bacteria form spherical blebs on their cell periphery, which later dissociate from the bacterial cell wall to form extracellular vesicles. These nano scale structures, known as outer membrane vesicles (OMVs), have been shown to promote infection and disease and can induce typical immune outputs in both mammal and plant hosts. To better understand the broad transcriptional change plants undergo following exposure to OMVs, we treated Arabidopsis thaliana (Arabidopsis) seedlings with OMVs purified from the Gram‐negative plant pathogenic bacterium Xanthomonas campestris pv. campestris and performed RNA‐seq analysis on OMV‐ and mock‐treated plants at 2, 6 and 24 h post challenge. The most pronounced transcriptional shift occurred at the first two time points tested, as reflected by the number of differentially expressed genes and the average fold change. OMVs induce a major transcriptional shift towards immune system activation, upregulating a multitude of immune‐related pathways including a variety of immune receptors. Comparing the response of Arabidopsis to OMVs and to purified elicitors, revealed that OMVs induce a similar suite of genes and pathways as single elicitors, however, pathways activated by OMVs and not by other elicitors were detected. Pretreating Arabidopsis plants with OMVs and subsequently infecting with a bacterial pathogen led to a significant reduction in pathogen growth. Mutations in the plant elongation factor receptor (EFR), flagellin receptor (FLS2), or the brassinosteroid‐insensitive 1–associated kinase (BAK1) co‐receptor, did not significantly affect the immune priming effect of OMVs. All together these results show that OMVs induce a broad transcriptional shift in Arabidopsis leading to upregulation of multiple immune pathways, and that this transcriptional change may facilitate resistance to bacterial infection.
Stagnation in quality of next‐generation sequencing assays for the diagnosis of hereditary hematopoietic malignancies
Authors: Roloff Gregory W. , Shaw Reid , O'Connor Timothy E. , Hathaway Feighanne , Drazer Michael W. 
Published: January 2023

AbstractHereditary hematopoietic malignancies (HHMs) are inherited syndromes that confer the risk of blood cancer development. With the rapid acceleration of next‐generation sequencing (NGS) into commercial biotechnology markets, HHMs are increasingly recognized by genetic counselors and clinicians. In 2020, it was demonstrated that most diagnostic test offerings for HHMs were insufficient for accurate diagnosis, failing to sequence the full spectrum of genetic events known to cause HHMs. We hypothesized the number of genes on commercially available HHM assay increased from 2020 to 2022, consistent with a more comprehensive sequencing approach. Here, we analyzed assays from eight commercial laboratories to determine the HHM‐related genes sequenced by these assays. We compared these assays with panels from 2020 to determine trends in sequencing quality. Most HHM diagnostic assays did not change and remain insensitive for the detection of all HHM‐related variants. Most (75%) HHM assays do not sequence CHEK2, the gene most frequently mutated in HHMs, and 25% of HHM assays does not sequence DDX41, the second most frequent HHM driver. The quality of HHM diagnostic assays stagnated despite the discovery of novel HHM‐related genes and prior work demonstrating heterogeneity in the quality of HHM testing. Most commercially available HHM tests remain insufficient.
Effect of Averaging Measurements From Multiple MRI Pulse Sequences on Kidney Volume Reproducibility in Autosomal Dominant Polycystic Kidney Disease
Authors: Dev Hreedi , Zhu Chenglin , Sharbatdaran Arman , Raza Syed I. , Wang Sophie J. , Romano Dominick J. , Goel Akshay , Teichman Kurt , Moghadam Mina C. , Shih George , Blumenfeld Jon D. , Shimonov Daniil , Chevalier James M. , Prince Martin R. 
Published: January 2023

BackgroundTotal kidney volume (TKV) is an important biomarker for assessing kidney function, especially for autosomal dominant polycystic kidney disease (ADPKD). However, TKV measurements from a single MRI pulse sequence have limited reproducibility, ± ~5%, similar to ADPKD annual kidney growth rates.PurposeTo improve TKV measurement reproducibility on MRI by extending artificial intelligence algorithms to automatically segment kidneys on T1‐weighted, T2‐weighted, and steady state free precession (SSFP) sequences in axial and coronal planes and averaging measurements.Study TypeRetrospective training, prospective testing.SubjectsThree hundred ninety‐seven patients (356 with ADPKD, 41 without), 75% for training and 25% for validation, 40 ADPKD patients for testing and 17 ADPKD patients for assessing reproducibility.Field Strength/SequenceT2‐weighted single‐shot fast spin echo (T2), SSFP, and T1‐weighted 3D spoiled gradient echo (T1) at 1.5 and 3T.Assessment2D U‐net segmentation algorithm was trained on images from all sequences. Five observers independently measured each kidney volume manually on axial T2 and using model‐assisted segmentations on all sequences and image plane orientations for two MRI exams in two sessions separated by 1–3 weeks to assess reproducibility. Manual and model‐assisted segmentation times were recorded.Statistical TestsBland–Altman, Schapiro–Wilk (normality assessment), Pearson's chi‐squared (categorical variables); Dice similarity coefficient, interclass correlation coefficient, and concordance correlation coefficient for analyzing TKV reproducibility. P‐value < 0.05 was considered statistically significant.ResultsIn 17 ADPKD subjects, model‐assisted segmentations of axial T2 images were significantly faster than manual segmentations (2:49 minute vs. 11:34 minute), with no significant absolute percent difference in TKV (5.9% vs. 5.3%, P = 0.88) between scans 1 and 2. Absolute percent differences between the two scans for model‐assisted segmentations on other sequences were 5.5% (axial T1), 4.5% (axial SSFP), 4.1% (coronal SSFP), and 3.2% (coronal T2). Averaging measurements from all five model‐assisted segmentations significantly reduced absolute percent difference to 2.5%, further improving to 2.1% after excluding an outlier.Data ConclusionMeasuring TKV on multiple MRI pulse sequences in coronal and axial planes is practical with deep learning model‐assisted segmentations and can improve TKV measurement reproducibility more than 2‐fold in ADPKD.Evidence Level2Technical EfficacyStage 1
In Situ Growth of Cyclodextrin‐Based Metal Organic Framework Air Filters for Reusable SO2 Adsorbent Applications
Authors: Kim Juran , Lee Jinwook , Kim Jooyoun 
Published: January 2023

AbstractIn situ growth of cyclodextrin‐based metal‐organic frameworks (CD‐MOFs) is investigated for the effective fabrication of MOF composites through the vapor diffusion method combined with O2 plasma treatment. The growth rate of γ‐CD‐MOFs on poly(ethylene terephthalate) fibers (CD‐MOFs–PET) is determined for 136 h, and the CD‐MOFs‐PET with 24 h growth time shows the highest surface area without crystal deformation. The specific surface area of the CD‐MOFs‐PET with 24 h growth time is 142.88 m2 g−1, which is 235 times higher than the pristine PET fibers. The 24 h CD‐MOFs‐PET shows removal efficiencies higher than 99% for PM0.1 and higher than 95% for SO2 gas. The CD‐MOFs‐PET demonstrates high recovery of SO2 adsorption ability by the repeated adsorption‐desorption cycles, maintaining up to 90% of initial adsorption performance. This study intends to provide an informative discussion of the applicability of textile‐based CD‐MOFs and the development of reusable filters for the removal of SO2.
Palliative radiotherapy for children: Symptom response and treatment‐associated toxicity according to radiation therapy dose and fractionation
Authors: Sudmeier Lisa J. , Madden Nicholas , Zhang Chao , Brock Katharine , Esiashvili Natia , Eaton Bree R. 
Published: January 2023

AbstractBackground/objectivesRadiotherapy is an effective palliative treatment in advanced cancer. Shorter palliative treatment courses are recommended for adults, though pediatric data addressing treatment efficacy and toxicity according to radiation therapy (RT) dose and fractionation are limited.Design/methodsTotal 213 patients aged 21 years or younger receiving 422 palliative radiotherapy treatment courses from 2003 to 2016 were included. Symptom response and treatment‐associated toxicity were recorded and analyzed in relationship to demographic and treatment variables.ResultsCommon diagnoses included sarcoma (32.5%), neuroblastoma (24.9%), leukemia/lymphoma (14.9%), and central nervous system tumors (10.9%). The most common indication for treatment was pain (46.7%). Patients received a median of 10 fractions, 2.5 Gy dose per fraction, and 21 Gy total dose. Number of RT fractions was five or less in 166 (39.3%), six to 10 fractions in 117 (27.2%), and 10 or more fractions in 139 (32.9%) of courses. Complete or partial pain relief was achieved in 85% (151 of 178 evaluable patients), including 77.8% receiving five or less fractions and 89.6% receiving more than five fractions. Highest toxicity was grade 1 in 159 (38.9%), grade 2 in 26 (6.4%), and grade 3 in two (0.5%) treatments. On multivariable analysis, RT delivered 30 or more days from death (OR 12.13, 95% CI: 2.13–69.2, p = .005) and no adjuvant chemotherapy (OR 0.14, 95% CI: 0.03–0.54, p = .005) were significantly associated with pain response, and five or less fractions were significantly associated with lower toxicity (OR 0.24, 95% CI: 0.06–0.97, p = .045).ConclusionsPalliative RT courses of five or less fractions result in high rates of pain control and are associated with low toxicity in pediatric patients with cancer.
A study on modified fabric‐contained melamine/bentonite polyurethane foam: Pb2+ adsorption and mechanical properties
Authors: Zhang Xiao , Lin Jia‐Horng , Li Ting‐Ting , Wang Zhi‐Ke , Shiu Bing‐Chiuan , Lou Ching‐Wen 
Published: January 2023

AbstractIn this study, a porous composite foam with excellent adsorption property toward Pb2+ was obtained by combining modified polyester (PET) fabric with polyurethane (PU) foam and using melamine and bentonite as modifiers. The effects of bentonite content on the adsorption and mechanical property of the modified fabric were studied. Based on the position of the modified PET fabric in porous PU foam, the P‐PU and PU‐P‐PU composite foams are prepared, and the effects of the fabric‐foam interface structure on the mechanical properties and adsorption properties were investigated. Effects of different experimental conditions such as pH value, contact time and initial dye concentration on adsorption capacity were investigated, and kinetic and isotherm of adsorption were used to analyze the adsorption mechanism of Pb2+ by foam. It was found that the porous composite foam have outstanding mechanical property and high adsorption efficiency. The adsorption was well‐described by pseudo‐second‐order kinetic and Langmuir isotherm models. Thus, the porous composite foam has great promise in removing heavy metal ions from water.
Highly exo‐olefinic polyisobutene synthesized using a recyclable acid
Authors: Lewis Stewart P. , Damodaran Krishnan 
Published: January 2023

AbstractScandium(III) and ytterbium(III) trifluoromethanesulfonate are active for the terminationless polymerization of moderate reactivity olefins (β‐pinene, isobutene, styrene) in conjunction with a variety of initiators (e.g., Brønsted acids, halogens, and carbocation synthons). Neither Lewis acid requires chlorinated/toxic solvents to operate. The Yb congener is quantitatively recovered by aqueous extraction, reactivated by dehydration, and maintains its activity after multiple reuses. Both acids produce good yields (up to ~100%) of polyisobutene with an average exo‐olefinic end‐group content for Sc(OTf)3 of 86% (Alfa Asear) and 75% (Aldrich), and 94% for Yb(OTf)3 (Aldrich). The molecular weights of poly(β‐pinene) and polyisobutene made by scandium(III) and ytterbium(III) trifluoromethanesulfonate, respectively, in combination with methanol are quite favorable at ambient temperature.
Signatures of Topological Surface State and Unconventional Magnetotransport Properties in Elemental Ruthenium
Authors: Singha Ratnadwip , Sarkar Shuvam , Ghosh Arup , Roy Shubhankar , Barman Sajal , Balal Mohammad , Barman Sudipta Roy , Mandal Prabhat 
Published: January 2023

AbstractIn recent years, the pursuit of new topological materials has created a vast and ever growing catalogue of compounds. However, the example of elemental topological metals is still rather limited. So far, the non‐trivial topological states have been probed in only a handful of elements and that too rarely in a transition metal. By combining the angle‐resolved photoemission spectroscopy (ARPES) and magnetotransport measurements, here, ruthenium (Ru) is experimentally confirmed to be a topological metal, validating a recent theoretical prediction. The ARPES data for Ru(0001) provide evidence of the topological surface state related to the bulk Dirac node. Ru also exhibits a prominent planar Hall effect, thus further indicating its Dirac semimetallic nature. Large electron and hole mobilities are estimated, which are comparable to several topological semimetals. The non‐trivial nature of electronic band of Ru is also supported by the large non‐saturating transverse magnetoresistance.