bluuebunny/crossref_metadata_2025_split huggingface dataset:
Embryoglycan ectodomains regulate biological activity of FGF-2 to embryonic stem cells
Authors: Dvořák P. , Hampl A. , Jirmanová L. , Pacholíková J. , Kusakabe M. 
Published: June 2023

ABSTRACT
Basic fibroblast growth factor (FGF-2) functions as a natural inducer of mesoderm, regulator of cell differentiation and autocrine modulator of cell growth and transformation. The FGF-2 signals are transduced through receptors with intrinsic protein tyrosine kinase activity. However, receptor binding and activation is governed by extracellular matrix, cell surface or soluble proteoglycans. This paper focuses on the role of proteoglycans synthesized by embryonic cells, embryoglycans, in FGF-2 signaling via FGF receptor-1 (FGFR-1).
We found that embryoglycan ectodomain Lewis X, analog of developmentally regulated embryonic cell surface epitope TEC 1, promotes oligomerization of FGF-2 in the cell free chemical crosslinking. In vitro assays show that a large molar excess of extracellular Lewis X does not inhibit binding of FGF-2 to embryonic stem (ES) cells, but prevents the mitogenic effect of FGF-2. Western blot analysis of ES cells revealed the presence of abundant 52 kDa and trace amounts of 67 and 125 kDa isoforms of FGFR-1. However, none of these isoforms undergo any detectable changes in tyrosine phosphorylation under the conditions that modulate the mitogenic effect of FGF-2. Rather, a primary substrate of all receptor tyrosine kinases, phospholipase Cγ (PLCγ), is activated by both FGF-2 and Lewis X. The combination, FGF-2 plus Lewis X, leads to weak inhibition, when compared with the effects of FGF-2 and Lewis X, respectively. In accordance, the level of phosphorylation of non-receptor tyrosine kinase c-Src is reduced in a reversed pattern to PLCγ. Furthermore, in this particular cell type we show the presence of activated forms of extracellular signal-related kinase (ERK) in all nontreated and treated cells.
These findings demonstrate that embryoglycan ectodomains may act as negative regulators of FGF-2-induced ES cell proliferation, most likely through the FGFR-1-independent signaling pathway.
Polarized localizations of annexins I, II, VI and XIII in epithelial cells of intestinal, hepatic and pancreatic tissues
Authors: Massey-Harroche Dominique , Mayran Nathalie , Maroux Suzanne 
Published: June 2023

ABSTRACT
The cellular and subcellular localizations of annexins I, II, VI and XIII in the rabbit intestine, liver and pancreas were studied by performing immunofluorescence labeling on thin frozen tissue sections using specific monoclonal antibodies. The expression of annexins was found to be finely regulated. Annexins XIII and I were expressed exclusively in the small intestine and the colon, respectively, whereas annexin II was present in all the tissues tested and annexin VI specifically in the liver and pancreas. These different annexins were concentrated in the basolateral domain of polarized cells, and some of them had an extra-apical localization: annexin XIII was concentrated in the lower 3/4 of enterocyte brush border microvilli; annexin II was present in the upper part of the terminal web in intestinal absorbent cells as well as in the bile canalicular area in hepatocytes, whereas annexin VI was detected on some apical vesicles concentrated around the bile canaliculi. In pancreatic acinar cells, the presence of annexin II on some zymogen granules provides further evidence that annexin II may be involved in exocytic events. In conclusion, this study shows that the basolateral domain of polarized cells appears to be the main site where annexins are located, and they may therefore be involved in the important cellular events occurring at this level.
Regulation of mRNA localization by transmembrane signalling: Local interaction of HB-GAM (heparin-binding growth-associated molecule) with the cell surface localizes β-actin mRNA
Authors: Fages Carole , Kaksonen Marko , Kinnunen Tarja , Punnonen Eeva-Liisa , Rauvala Heikki 
Published: June 2023

ABSTRACT
Localization of mRNAs is currently thought to be partially responsible for molecular sorting to specific compartments within the cell. In mammalian cells the best-studied example is the β-actin mRNA that is localized to the cell processes, and its localization is necessary in migratory responses of cells. It is reasonable to assume that mRNA localization within cells is coupled to transmembrane signalling due to extracellular factors, but little is known about such putative mechanisms. We show here that HB- GAM, an extracellular matrix-associated factor that enhances migratory responses in cells, is able to localize β- actin mRNA when locally applied to cells via microbeads. The HB-GAM-induced mRNA localization is specifically inhibited by low concentrations of heparin and by heparitinase treatment of cells, showing that cell-surface heparin-type glycans are required for the effect. The finding that soluble N-syndecan is also inhibitory suggests that the transmembrane proteoglycan N-syndecan, previously identified as an HB-GAM receptor, is involved in the mRNA-localizing effect of HB-GAM. Inhibition of the mRNA localization by the src-kinase inhibitor PP1 is compatible with an N-syndecan-mediated effect since the receptor function of N-syndecan has been recently found to depend on the src-kinase signalling pathway. The mRNA- localizing activity of N-syndecan is also suggested by the finding that affinity-purified anti-N-syndecan antibodies coated on microbeads are able to localize β-actin mRNA.
Ye1013p (Vac8p), an armadillo repeat protein related to plakoglobin and importin α, is associated with the yeast vacuole membrane
Authors: Fleckenstein Diana , Rohde Manfred , Klionsky Daniel J. , Rüdiger Manfred 
Published: June 2023

ABSTRACT
Proteins of the armadillo family are involved in diverse cellular processes in higher eukaryotes. Some of them, like armadillo, β-catenin and plakoglobins have dual functions in intercellular junctions and signalling cascades. Others, belonging to the importin-α-subfamily are involved in NLS recognition and nuclear transport, while some members of the armadillo family have as yet unknown functions.
Here, we introduce the Saccharomyces cerevisiae protein Ye1013p as a novel armadillo (arm) repeat protein. The ORF Ye1013w was identified in the genome project on chromosome V (EMBL: U18530) and codes for an acidic protein of 578 residues with 8 central arm-repeats, which are closely related to the central repeat-domain of Xenopus laevis plakoglobin. We show that Ye1013p (Vac8p) is constitutively expressed in diploid and haploid yeasts and that it is not essential for viability and growth. However, the vacuoles of mutant cells are multilobular or even fragmented into small vesicles and the processing of aminopeptidase I, representing the cytoplasm-to-vacuole transport pathway, is strongly impaired. Consistent with these observations, subcellular fractionation experiments, immunolocalization and expression of green fluorescent protein (GFP) fusion proteins revealed that Ye1013p (Vac8p) is associated with the vacuolar membrane.
Our data provide evidence for the involvement of an arm-family member in vacuolar morphology and protein targeting to the vacuole.
Integrin ligation and PKC activation are required for migration of colon carcinoma cells
Authors: Rigot Véronique , Lehmann Maxime , André Frédéric , Daemi Noucha , Marvaldi Jacques , José Luis 
Published: June 2023

ABSTRACT
The activation of protein kinases C (PKCs) is an essential step in integrin-dependent cell adhesion and spreading. In this report we examined the effect of the phorbol ester PMA, a PKC activator, on adhesion, spreading and migration of a colon carcinoma cell line, HT29-D4. Treatment with PMA increased the rate of cell spreading and induced the migration of these cells towards purified matrix proteins in haptotaxis assays on Boyden chambers. PMA-induced effects were the result of PKCs activation, as shown by using the inactive isomer 4α-PMA and PKCs inhibitors. The involvement of integrins in the phorbol ester-induced cell migration was demonstrated both by the absence of migration of cells plated on membranes coated with poly-L-lysine and by the use of function blocking antibodies. Thus, interactions between α2β1, α3β1, α6β4, αvβ5, αvβ6 integrins and their specific ligands are necessary for the PKC-mediated migration. However, adhesion, immunoprecipitation and immunocytofluorometry experiments clearly showed that HT29-D4 cell haptotaxis induced by PKC activation is not a consequence of quantitative or qualitative changes in the cell surface integrins. We also demonstrated that PKCs were able to activate the MAP kinase pathway and that the impediment of MAP kinase activation resulted in the loss of cell migration. Moreover, stimulation of the insulin-like growth factor I signalling pathway led to MAP kinase activation and to the induction of cell migration. In addition, the growth factor-induced motility of HT29-D4 cells was affected both by PKC and MAP kinase cascade inhibitors. It thus appears that both integrin ligation and MAP kinase activation by PKCs are required to promote the migration of HT29-D4 cells.
Effect of para-net shade level on plant microenvironment, growth, and yield of three strawberry varieties
Authors: SUMINARTI NUR EDY , SEBAYANG HUSNI THAMRIN , MAGHFOER MOHAMAD DAWAM , BULAN BULAN 
Published: June 2023

Abstract. Suminarti NE, Sebayang HT, Maghfoer MD, Bulan. 2023. Effect of para-net shade level on plant microenvironment, growth, and yield of three strawberry varieties. Biodiversitas 24: 2149-2155. Plant environmental management through the application of para-net shade and the selection of adaptive varieties for strawberry plants has been carried out from March to June 2020 in Jatikerto.The study used a split-plotdesign by placing shade treatments on the main plot consisting of four types, namely without shade (0%), 25% shading, 50% shading, and 75% shading, while the variety treatment was placed in subplots consisting of three kinds, namely Sweet Charlie, California, and Earlibrite varieties.The 5% F test was used to determine the interaction or significant effect of the treatments, while the 5% HSD test was used to determine differences between treatments.The results showed that there was a significant interaction in observing fruit weight per hectare. In the treatment without shade and with 25% shade, the highest fruit weight was produced by the California variety, 1.06 t ha-1,and 0.80 t ha-1, respectively. Whereas at 50% shade, the highest fruit weight per hectare was found in the Sweet Charlie variety, namely 0.66 t ha-1, and at 75% shade, higher yields were found in the Sweet Charlie and Earlibrite varieties, each of 0.65 t ha-1and 0.55 t ha1. Conclusion: California is a fairly tolerant variety to be developed in the Jatikerto area.
Age, growth, mortality, and population characteristics of the red snapper (Lutjanus malabaricus) in the Timor Sea waters, Indonesia
Authors: HERWATY SUSY , MALLAWA ACHMAR , NAJAMUDDIN NAJAMUDDIN , ZAINUDDIN MUKTI 
Published: June 2023

Abstract. Herwaty S, Mallawa A, Najamuddin, Zainuddin M. 2023. Age, growth, mortality, and population characteristics of the red snapper (Lutjanus malabaricus) in the Timor Sea waters, Indonesia. Biodiversitas 24: 2217-2224. Red snapper (Lutjanus malabaricus Bloch & Schneider 1801) is a fishery resource with export commodities, high market demand is suspected of causing overfishing and a decrease in stocks, thus threatening its sustainability. This study aims to analyze the red snapper's age, growth, mortality, and population characteristics. Fish samples were collected at the Oeba Fish Landing Base Kupang from April to June 2020. The data analysis were as follows: the descriptively analyzed size structure, age group using the Bhattacharya method, growth parameter values (L? and K) based on the von Bertalanffy equation, and natural mortality using the Pauly and Y/R using the Beverton and Holt methods. The results of the red snapper observation are as follows: the smallest size at 12.8 cm (FL), the largest size at 72.8 cm (FL), the average length at 42.03 cm (FL) ± 16.03, the growth parameter value (L?)=99.40 cm (FL), (K)=0.51 year-1, t0=-0.23018 year-1, the population consisted of two and three age groups, the total mortality value (Z) 1.98 year-1, natural mortality (M) 0.84 year-1, fishing mortality (F) 1.14 year-1, and exploitation rate (E) 0.58 year-1. The actual Y/R value was 0.049, and the relative Y/R was 0.060 grams recruit-year-1. The fishing mortality is higher than the natural mortality value; a high exploitation value (E>0.5) indicates that over-exploitation and stock conditions are unhealthy. The results of this study can be used as a reference in managing red snapper resources in the future.
Metabolomic and sensomic approach to characterize key aroma of aromatic mangoes from Indonesia
Authors: BELGIS MARIA , WITONO YULI , GIYARTO GIYARTO , SADEK NUR FATHONAH , MASRUROH ELOK LUTFI , HERLINA HERLINA 
Published: June 2023

Abstract. Belgis M, Witono Y, Giyarto, Sadek NF, Masruroh EL, Herlina. 2023. Metabolomic and sensomic approach to characterize key aroma of aromatic mangoes from Indonesia. Biodiversitas 24: 2242-2250. Garifta Merah, Garifta Orange, and Agri Gardina 45 are new mango cultivars with a strong aroma and attractive color from Indonesia. This study investigated aromatic constituents and sensory profiles using a combination of metabolomic and sensomic approaches of Garifta Merah, garifta Orange, and Gardina 45. Volatile compounds were extracted using Head Space-Solid Phase Microextraction (HS-SPME), then identified using Gas Chromatography-Mass Spectrometry (GC-MS). Sensory descriptive Rate All That Apply (RATA) and hedonic tests were used for sensory attributes profiling and aroma-liking evaluation. Monoterpene hydrocarbons were the predominant volatile compound in all cultivars; however, it was also accompanied by esters in Garifta Orange and Agri Gardina 45. The Principal Component Analysis (PCA) classified those cultivars into different groups. Garifta Merah was characterized by higher ?-pinene, while Garifta Orange was characterized by high ethyl butanoate. Agri Gardina was in the separate group characterized by ?-trans ocimene, ethyl butanoate, and isopentyl butanoate. The identified compounds in all cultivars had Odor Active Value (OAV)>1 and correlated to their sensory profile described by assessors. Garifta Orange was perceived to have the highest overall aroma liking among all cultivars. It was presumably due to the high ethyl butanoate content related to the pineapple, mango, fruity, and fermented attributes.
The importance of DNA barcode reference libraries and selection primer pair in monitoring fish diversity using environmental DNA metabarcoding
Authors: ROESMA DEWI IMELDA , TJONG DJONG HON , SYAIFULLAH SYAIFULLAH , NOFRITA NOFRITA , JANRA MUHAMMAD NAZRI , PRAWIRA FURQAN DWIKI LINTANG , SALIS VIOLA MUTIARA , AIDIL DYTA RABBANI 
Published: June 2023

Abstract. Roesma DI, Tjong DH, Syaifullah, Nofrita, Janra MN, Prawira FDL,Salis VM, Aidil DR. 2023.The importance of DNA barcode reference libraries and selection primer pair in monitoring fish diversity using environmental DNA metabarcoding. Biodiversitas 24: 2251-2260. Environmental DNA (eDNA) metabarcoding has become an alternative method used for biodiversity monitoring of an ecosystem. The eDNA metabarcoding has advantages compared to the conventional method because it is non-invasive, quick, and requires less cost. However, the effectiveness of the eDNA method is highly dependent on the coverage of the DNA barcode reference and primerpair. A study using the eDNA method was conducted for fish biodiversity monitoring in Singkarak Lake. Two-liter water samples were collected using sterile bottle samples at each sampling site (five sites). The universal primers (Fish FI and Fish R1) used for Next-generation sequencing (GRIDION, Nanopore, Oxford Technologies). The study detected 152 fish species using eDNA metabarcoding. Ten species out of the 30 originally reported in Singkarak Lake were detected using eDNA metabarcoding. The low percentage of fish detected is thought to be due to several factors; incomplete/unavailability of freshwater fish DNA barcodes in Indonesia registered in the database repository, inappropriate primer pair selection, low DNA quality, and the absence of target species DNA in collected water samples. The results demonstrated the significance of correctly registering DNA barcodes to the database and appropriate primer pair selection to identify eDNA metabarcoding. This study provides recommendations using eDNA metabarcoding for monitoring in future work.
Nanoscale plasmonic combinational logic circuits based on an elliptical resonator
Authors: Alali Mohammed J. , Raheema Mithaq Nama , Alwahib Ali A. 
Published: June 2023

The half-adder (HA) and half-subtractor (HS) plasmonic combinational
logic circuits are explained using a finite element method with a
COMSOL software package. The combinational circuits are created using
insulator-metal-insulator technology with nanoscale plasmonic
structures. In order to achieve an excellent transmission value, the
phase angle of optical waves and the position of the control and input
ports are the more crucial elements. In this design, the nanoscale
combinational circuits are realized at a 35% transmission threshold to
distinguish between the logic “0” and logic “1” stand on the
interference between the input and control ports with 540nm×250nm dimensions and an 850 nm
resonant wavelength. The modulation depth, contrast ratio, and
insertion loss have 97.38%, and 11.84 and 3.3 dB for the HA,
and they have 92.38%, and 7.12 and −1.41dB for the HS, respectively.
Spatial analysis of the epidemiological risk of leprosy in the municipalities of Minas Gerais
Authors: Bueno Isabela de Caux , Lages Daniele dos Santos , Lana Francisco Carlos Felix 
Published: June 2023

Background
Leprosy remains a significant public health problem of high importance. This investigation aims to analyze the spatial distribution of the leprosy epidemiological risk in the municipalities of Minas Gerais.

Methods
This ecological study was conducted with new leprosy cases diagnosed from 2004 to 2019 in the municipalities of the state of Minas Gerais. Based on the epidemiological indicators, a composite indicator called the leprosy epidemiological risk index was estimated, classifying municipalities as high, medium, low and very low risk. For the spatial analysis, the global and local spatial autocorrelation statistics were used to identify the spatial distribution of the leprosy epidemiological risk in the periods 2004–2011 and 2012–2019 and classified as High/High, Low/Low, High/Low and Low /High.

Results
Although leprosy is declining in the state of Minas Gerais, the Global Moran Index confirmed the spatial dependence between municipalities for the two analyzed periods, characterizing the formation of clusters. When performing the local spatial autocorrelation, it was found that the macroregions with the highest number of municipalities with high indices, surrounded by other municipalities with high indices (high-high), were Northwest, East, South East, North, and Northeast. The low risk macroregions were Southeast, Center, South-Center and South.

Conclusion
Leprosy has a heterogeneous spatial pattern and remains concentrated in historically endemic areas of the state. It underscores the importance of intensifying actions to combat leprosy in these municipalities and macroregions. Promote improved access to health services and combat stigma and prejudice to eliminate leprosy as a public health problem.
Prevalence of HIV, risk behaviours and vulnerabilities of female sex partners of the HIV positive people who inject drugs (PWID) in Dhaka city, Bangladesh
Authors: Reza Md. Masud , Rana A. K. M. Masud , Morshed Khan Mohammad Niaz , Safiullah Sarker Md. , Chowdhury Sujan , Uddin Md. Ziya , Rahman Lima , Chowdhury Mohammad Ezazul Islam , Taher Md. Abu , Khan Sharful Islam 
Published: June 2023

Background
The HIV epidemic in Bangladesh is largely being driven by people who inject drugs (PWID) and mainly concentrated in Dhaka city. Intregrated biological and behavioural survey (IBBS) data of 2016 showed that a considerable percentage of the HIV positive PWID had unsafe sex with their female sex partners. Prevalence of HIV, risk behaviorus and vulnerabilities among the female sex partners of the PWID still remain unexplored.

Methods
To measure HIV prevalence, risk behaviours (drugs/injection/sexual) and vulnerabilities (treatment of and knowledge of sexually transmitted infections (STIs) and HIV/uptake of the routine HIV testing and HIV prevention services/physical and sexual violence), a quantitative survey was conducted among 227 female sex partners of the HIV positive PWID in Dhaka city in 2019 by adopting a take-all sampling technique.

Results
The median age of participants was 34.0 years. Prevalence of HIV was 16.7% (95% CI: 12.4–22.2). Only 6.8% to 18.7% of the participants used condoms consistently with different male sex partners; only 6.8%cto 18.7% during last year. Seventy five percent (95% CI: 69.2–80.8) had no knowledge on STI symptoms. Self-reported symptoms of STIs were reported by 26% (95% CI: 20.7–32.1) and half sought treatment during last year. Nineteen percent (95% CI: 14.7–25.1) had comprehensive knowledge of HIV. As part of the routine HIV prevention services by the PWID drop-in-centres (DICs), 42.7% (95% CI: 36.4–49.3) of the participants were tested for HIV and knew their result within the last year. One-third never received HIV prevention services. During the last one year preceding the survey, 46% (95% CI: 39.3–52.6) reported been beaten and 20.2% (95% CI: 15.3–26.1) been raped.

Conclusion
It is urgently necessary to consider the high-risk behaviours and vulnerabilities in designing or to strengthen targeted interventions for female sex partners of the HIV positive PWID in Dhaka city to ensure equality in accessing and utilization of services.
Agnosti Chora: Ellada kai Dysi stis Arches tou 20ou Aiona (‘Unknown country: Greece and the west at the beginning of the twentieth century’), Effi Gazi (2020)
Authors: Pateridou Georgia 
Published: June 2023

Review of: Agnosti Chora: Ellada kai Dysi stis Arches tou 20ou Aiona (‘Unknown country: Greece and the west at the beginning of the twentieth century’), Effi Gazi (2020)
Athens: Polis, 354 pp.,
ISBN 978-960-435-735-2, p/bk, €20
Public Subsidies and Cooperation in Research and Development. Evidence from the LAB
Authors: Acconcia Antonio , Beraldo Sergio , Capuano Carlo , Stimolo Marco 
Published: June 2023

Abstract
We implement an experimental design based on a duopoly game in order to analyse the impact of public subsidies on the willingness to cooperate in research and development (R&D) activities. We first implement six experimental markets by exogenously varying the level of knowledge spillovers (low or high) and the intensity of competition in the product market (low, intermediate, or high). We find that the probability of cooperation increases in the level of spillovers and decreases in that of market competition. We then replicate the six experimental markets by subsidising subjects who cooperate. Whenever they are sufficient to change the incentive structure of the game, subsidies substantially increase the probability of cooperation, causing, however, a reduction of R&D investments. Overall, our evidence suggests that, depending on the characteristics of the market, the provision of subsidies is not always desirable. These might be redundant because firms have sufficient private incentives to invest cooperatively in R&D, or even counterproductive, as they might induce firms to significantly reduce R&D investments compared to the noncooperative scenario.