bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neutrophil Extracellular Trap–Borne Elastase Prevents Inflammatory Relapse in Intercritical Gout
Authors: Liu Lei , Shan Lizhen , Wang Han , Schauer Christine , Schoen Janina , Zhu Lingjiang , Lu Ci , Wang Zitao , Xue Yu , Wu Huaxiang , Herrmann Martin 
Published: December 2022

ObjectiveGout flares that occur during urate‐lowering therapy (ULT) are typically related to the shrinkage of tophi due to aggregated neutrophil extracellular traps (NETs) that have captured monosodium urate crystals in the tissues. The present study was undertaken to analyze the blocking effect of α1‐antitrypsin on neutrophil elastase, and it was found that α1‐antitrypsin induced rapid inflammation in the presence of unstable tophi.MethodsCell‐free DNA levels in serum samples were compared between patients who experienced a varying number of gout flares. We investigated whether cell‐free DNA in serum samples and α1‐antitrypsin could be altered after the initiation of ULT. In mice, an injection of monosodium urate monohydrate (MSU) crystals was used to form a mimic of tophi in the peritoneal cavity, which was then analyzed using immunofluorescence staining. Finally, we investigated the relapse of inflammation by analyzing the levels of α1‐antitrypsin in 2 kinds of artificial tophi and in tophus‐bearing mice.ResultsLevels of cell‐free DNA in serum samples correlated with the number of flares experienced by patients with tophaceous gout. ULT induced an increase in cell‐free DNA in the serum of patients with tophi. Increases in levels of α1‐antitrypsin were seen in patients with tophi who received ULT. Chalk‐like tophi removed from the peritoneal cavity of mice after MSU crystals induced inflammation showed abundant coexpression of interleukin‐1β (IL‐1β) and IL‐6–associated NETs. A relapse in inflammation was induced by α1‐antitrypsin during the spontaneous resolution of MSU crystal–induced peritonitis. We observed that α1‐antitrypsin blocks cytokine degradation by neutrophil elastase during the resolution phase of tophi.ConclusionULT causes shrinkage of the tophi reflected by an increase in the levels of cell‐free DNA in serum. In the resolution phase of tophi in mice, NET‐associated neutrophil elastase degrades proinflammatory cytokines and, thus, ameliorates inflammation.
Designing High‐Performing Symmetric Supercapacitor by Engineering Polyaniline on Steel Mesh Surface via Electrodeposition
Authors: Mahfoz Wael , Das Himadri Tanaya , Shah Syed Shaheen , Sanhoob Mohammed , Anjum Ahtisham , Al‐Betar Abdul‐Rahman , Aziz Md. Abdul 
Published: December 2022

AbstractEnergy storage is one of the most stimulating requirements to keep civilization on the wheel of progress. Supercapacitors generally exhibit a high‐power density, have a maximum life cycle, quick charging time, and are eco‐friendly. Polyaniline (PANI), a conductive polymer, is considered an efficacious electrode material for supercapacitors due to its good electroactivity, including pseudocapacitive behavior. Here, we present the fabrication of a symmetric supercapacitor device based on steel mesh electrodeposited with PANI. Due to its effective conductivity, porous nature, and low cost, steel mesh is a good choice as a current collector to fabricate a high‐performance supercapacitor at a low cost. The optimum fabricated supercapacitor has a high specific capacitance of ∼353 mF cm−2. Furthermore, the supercapacitor obtained an energy density of ∼26.4 μW h cm−2at a power density of ∼400 μW cm−2. The fabricated supercapacitor shows high stability, as the initial capacitance remained almost the same after 1000 charge/discharge cycles. PANI is a promising candidate for mass production and wide applications due to its low cost and high performance.
A high‐throughput expression and screening platform for applications‐driven PETase engineering
Authors: Zurier Hannah S. , Goddard Julie M. 
Published: December 2022

AbstractThe environmental consequences of plastic waste have impacted all kingdoms of life in terrestrial and aquatic ecosystems. However, as the burden of plastic pollution has increased, microbes have evolved to utilize anthropogenic polymers as nutrient sources. Of depolymerase enzymes, the best characterized is PETase, which hydrolyzes aromatic polyesters. PETase engineering has made impressive progress in recent years; however, further optimization of engineered PETase toward industrial application has been limited by lower throughput techniques used in protein purification and activity detection. Here, we address these deficiencies through development of a higher‐throughput PETase engineering platform. Secretory expression via YebF tagging eliminates lysis and purification steps, facilitating production of large mutant libraries. Fluorescent detection of degradation products permits rapid screening of depolymerase activity in microplates as opposed to serial chromatographic methods. This approach enabled development of more stable PETase, semi‐rational (SR) PETase variant containing previously unpublished mutations. SR‐PETase releases 1.9‐fold more degradation products and has up to 7.4‐fold higher activity than wild‐type PETase over 10 days at 40°C. These methods can be adapted to a variety of chemical environments, enabling screening of PETase mutants in applications‐relevant conditions. Overall, this work promises to facilitate advancements in PETase engineering toward industrial depolymerization of plastic waste.
Direct production of 4‐hydroxybenzoic acid from cellulose using cellulase‐displaying Pichia pastoris
Authors: Inokuma Kentaro , Miyamoto Shunya , Morinaga Kohei , Kobayashi Yuma , Kumokita Ryota , Bamba Takahiro , Ito Yoichiro , Kondo Akihiko , Hasunuma Tomohisa 
Published: December 2022

Abstract4‐hydroxybenzoic acid (4‐HBA) is an industrially important aromatic compound, and there is an urgent need to establish a bioprocess to produce this compound in a sustainable and environmentally friendly manner from renewable feedstocks such as cellulosic biomass. Here, we developed a bioprocess to directly produce 4‐HBA from cellulose using a recombinant Pichia pastoris strain that displays heterologous cellulolytic enzymes on its cell surface via the glycosylphosphatidylinositol (GPI)‐anchoring system. β‐glucosidase (BGL) from Aspergillus aculeatus, endoglucanase (EG) from Trichoderma reesei, and cellobiohydrolase (CBH) from Talaromyces emersonii were co‐displayed on the cell surface of P. pastoris using an appropriate GPI‐anchoring domain for each enzyme. The cell‐surface cellulase activity was further enhanced using P. pastoris SPI1 promoter‐ and secretion signal sequences. The resulting strains efficiently hydrolyzed phosphoric acid swollen cellulose (PASC) to glucose. Then, we expressed a highly 4‐HBA‐resistant chorismate pyruvate‐lyase (UbiC) from Providencia rustigianii in the cellulase‐displaying strain. This strain produced 975 mg/L of 4‐HBA from PASC, which corresponding to 36.8% of the theoretical maximum yield, after 96 h of batch fermentation without the addition of commercial cellulase. This 4‐HBA yield was over two times higher than that obtained from glucose (12.3% of the theoretical maximum yield). To our knowledge, this is the first report on the direct production of an aromatic compound from cellulose using cellulase‐displaying yeast.
Hard carbon anodes derived from phenolic resin/sucrose cross‐linking network for high‐performance sodium‐ion batteries
Authors: Xu Ran , Sun Ning , Zhou Huanyu , Chang Xiaqing , Soomro Razium A. , Xu Bin 
Published: December 2022

AbstractHard carbons are widely studied as anode materials for sodium‐ion batteries (SIBs) due to their high Na‐storage capacity, long cycle life, and low cost. However, the low initial coulombic efficiency (ICE) and poor cycle performance remain bottleneck concerns that necessitate a comprehensive material engineering solution. Herein, we propose a facile strategy to synthesize amorphous carbons with pseudo‐graphitic dominated crystalline, expanded interlayer spacing, and reduced surface defects via carbonization of the cross‐linking network of phenolic resin and sucrose. An elaborate structural and electrochemical characteristics analysis has been investigated against different sucrose contents and carbonization temperatures. The representative PF‐S‐55‐1200 with the optimum cross‐linking degree as well as carbonization temperature realizes a high reversible Na‐storage capacity of 323.0 mAh g−1 with an ICE as high as 86.4%, much superior to the pristine phenolic resin pyrolytic carbon with a capacity of 267.1 mAh g−1 and an ICE of 46.3%. The hybrid hard carbons also exhibit robust structural stability with a prolonged cycle lifespan evidenced by a retained capacity of 238.3 mAh g−1 at a current density of 200 mA g−1 over 1500 cycles. The proposed route promises low‐cost and high‐performance hybrid hard carbons with optimized structural configuration for advanced SIBs.
Anatomy of small canals around the jugular foramen: Special reference to Jacobson's and Arnold's nerves
Authors: Komune Noritaka , Suzuki Tomoharu , Miyamoto Yusuke , Iwanaga Joe , Matsuo Satoshi , Akiyama Osamu , Tubbs R. Shane , Nakagawa Takashi 
Published: December 2022

AbstractThe jugular foramen harbors anatomically complex bony, venous and neural structures. It is closely associated with small canals including the mastoid, tympanic, and cochlear canaliculi, and the stylomastoid foramen. The minute intraosseous branches of Arnold's and Jacobson's nerves (<1 mm in length) remain difficult to study with current imaging techniques, and cadaveric dissection is the most reliable approach. Our aim was to examine the variations of Jacobson's and Arnold's canaliculi and nerves and to provide detailed cadaveric graphics. To reveal the anatomical structures of small canals around the jugular foramen, 25 sides of dry skulls and 14 sides of cadaveric heads were examined. Intraosseous branches varied more in Arnold's nerve than Jacobson's nerve. In our cadaveric dissection, all specimens formed a single canal for Jacobson's nerve connecting the jugular foramen to the tympanic cavity. The intraosseous course of Arnold's nerve varied in its communication with the facial nerve. A descending branch crossing the facial nerve was identified in five of 14 sides, an ascending branch in 13. In two specimens, an ascending branch clearly reached the base of the stapedius muscle. Classical anatomical studies of cadavers remain a supplementary tool for analyzing these tiny structures. The present study confirms Gray's findings of 1913. Variations of these nerves could be even more complex than previously reported. Our study provides additional information regarding the anatomy of Jacobson's and Arnold's nerves.
Adipose and lipoma stem cells: A donor‐matched comparison
Authors: Conte Francesco , Beier Justus P. , Ruhl Tim 
Published: December 2022

AbstractLipomas are slow growing benign fat tumors that develop in soft tissues of the mesoderm. Thus, the specific (dys‐)function of mesenchymal stem cells (MSCs) has been suggested in the development of lipomas, but details of the tumor pathogenesis remain unclear. Existing studies comparing stem cells from native adipose (adipose stem cells [ASCs]) and lipomatous tissues (LSCs) have reported contradicting findings. However, harvesting ASCs and LSCs from different individuals might have influenced proper comparison. Therefore, we aimed to characterize donor‐matched ASCs and LSCs to investigate metabolic activity, proliferation, capability for tri‐linear differentiation (chondrogenesis, adipogenesis, osteogenesis), and the secretome of mature adipocytes and lipomacytes. Both stem cell types did not differ in metabolic activity, but ASCs demonstrated stronger proliferation than LSCs. While there was no difference in proteoglycan accumulation during chondrogenic differentiation, adipogenesis was higher in ASCs, with more lipid vacuole formation. Conversely, LSCs showed increased osteogenesis by higher calcium deposition. Lipomacytes showed stronger secretory activity and released higher levels of certain adipokines. Our findings indicated that LSCs possessed important characteristics of MSCs, including ASCs. However, LSCs' low proliferation and adipogenic differentiation behavior did not appear to account for enhanced tissue proliferation, but the secretome of lipomacytes could contribute to lipomatous neoplasm.
A successful Vasovasostomy operation
Authors: Dorjey Yeshey 
Published: December 2022

AbstractVasectomy is performed as a permanent method of contraception for men after completing the family. Many men are seeking vasectomy reversal operations after remarrying new partners. The reversal operation is seen as a challenging procedure that requires a skilled surgeon and sophisticated instruments. This write‐up reports a case of vasovasostomy performed successfully on an elderly man using the minimal available facilities at Phuentsholing General Hospital, Bhutan.
Dispersibility of TiO2 Nanoparticles in Less Polar Solvents: Role of Ligand Tail Structures
Authors: Sudo Tatsuya , Yamashita Shohei , Koike Natsumi , Kamiya Hidehiro , Okada Yohei 
Published: December 2022

AbstractNanoparticles (NPs) are inherently prone to aggregation and loss of their size‐derived properties, thus it is essential to enhance their dispersibility for applications. In less polar solvents, organic ligands containing oleyl groups are known as good dispersants due to their inefficient shell packing and inhibition of chain–chain crystallization as well as interdigitation between adjacent NPs. However, reagents with oleyl structures, such as oleic acid and oleylamine, can contain trans double bonds and saturated impurities, which might affect the chemical and/or physical properties of the NPs. Nevertheless, the effect of slight differences in surface ligand structure, including isomers, on the dispersibility of NPs has been little studied. We have synthesized five phosphonic acid ligands to investigate the structure–dispersibility relationship in detail. Dynamic light scattering and visible light transmittance revealed that not only regio‐ but also the stereochemistries of the C=C double bond in the ligand molecule, as well as the choice of solvent, are key factors in enhancing dispersibility.
Ab Initio Metadynamics Simulations of Hexafluoroisopropanol Solvent Effects: Synergistic Role of Solvent H‐Bonding Networks and Solvent‐Solute C−H/π Interactions
Authors: Hu Xinmin , Zhao Xia , Lv Xiangying , Wu Yan‐Bo , Bu Yuxiang , Lu Gang 
Published: December 2022

AbstractThe solvent effects in Friedel–Crafts cycloalkylation of epoxides and Cope rearrangement of aldimines were investigated by using ab initio molecular dynamics simulations. Explicit molecular treatments were applied for both reactants and solvents. The reaction mechanisms were elucidated via free energy calculations based on metadynamics simulations. The results reveal that both reactions proceed in a concerted fashion. Key solvent‐substrate interactions are identified from the structures of transition states with explicit solvent molecules. The remarkable promotion effect of hexafluoroisopropanol solvent is ascribed to the synergistic effect of H‐bonding networks and C−H/π interactions with substrates.
Fine‐needle aspiration cytology for the diagnosis of plasma cell neoplasms in the head and neck region: A systematic analysis of the literature
Authors: Felix Fernanda Aragão , de Sena Ana Carolina Velasco Pondé , de Arruda José Alcides Almeida , Tavares Thalita Soares , Rocha Amanda Leal , Rodrigues‐Fernandes Carla Isabelly , de Cáceres Cinthia Verónica Bardález López , Vargas Pablo Agustin , Abreu Lucas Guimarães , Amaral Tania Mara Pimenta , Travassos Denise Vieira , de Sousa Silvia Ferreira , Fonseca Felipe Paiva , Silva Tarcília Aparecida , Mesquita Ricardo Alves 
Published: December 2022

AbstractBackgroundCytopathologic analysis is feasible and provides detailed morphological characterisation of head and neck lesions.AimsTo integrate the available data published on fine‐needle aspiration cytology (FNAC) used for the diagnosis of plasma cell neoplasms (PCN) of the head and neck region.Materials and MethodsSearches on PubMed, Web of Science, Embase, and Scopus were performed to compile data from case reports/case series published in English. The Joanna Briggs Institute tool was used for the critical appraisal of studies.ResultsA total of 82 studies comprising 102 patients were included in this review. There was a predilection for men (68.6%) (male/female ratio: 2.1:1). Individuals in their 50s (29.4%), 60s (22.5%), and 70s (22.5%) were more often affected. The thyroid gland (26.2%) was the main anatomical location, followed by scalp (15.5%), neck/cervical region (15.5%), jaws (13.6%), and major salivary glands (13.6%). For FNAC analysis, a smear was employed in 41 (40.6%) cases and a cell block was used in four (3.9%). In 56 (55.4%) reports, no cytological methods were available. Morphologically, 34 (56.7%) cases had a diagnosis of PCN with agreement between cytopathology and histopathology. The rate of wrong diagnoses when using cytology was 27.5%. Immunophenotyping was performed in 49 (48%) of the cases. The 69‐month disease‐free survival rate was 60.2%, while the 27‐month overall survival rate was 64.1%.ConclusionThis study reinforces that FNAC can be an ancillary tool in the first step towards the diagnosis of PCN of the head and neck region, especially when applying a cell block for cytological analysis.
Expression dynamics of lymphoid enhancer‐binding factor 1 in terminal Schwann cells, dermal papilla, and interfollicular epidermis
Authors: Song Hongzhi , Zhao Xu‐Bo , Chu Qing‐Song , Zhang Jianyu , Gao Lipeng , Liao Xin‐Hua 
Published: December 2022

AbstractBackgroundTranscription factor lymphoid enhancer‐binding factor 1 (LEF1) is a downstream mediator of the Wnt/β‐catenin signaling pathway. It is expressed in dermal papilla and surrounding cells in the hair follicle, promoting cell proliferation, and differentiation.ResultsHere, we report that LEF1 is also expressed all through the hair cycle in the terminal Schwann cells (TSCs), a component of the lanceolate complex located at the isthmus. The timing of LEF1 appearance at the isthmus coincides with that of hair follicle innervation. LEF1 is not found at the isthmus in the aberrant hair follicles in nude mice. Instead, LEF1 in TSCs is found in the de novo hair follicles reconstituted on nude mice by stem cells chamber graft assay. Cutaneous denervation experiment demonstrates that the LEF1 expression in TSCs is independent of nerve endings. At last, LEF1 expression in the interfollicular epidermis during the early stage of skin development is significantly suppressed in transgenic mice with T‐cell factor 3 (TCF3) overexpression.ConclusionWe reveal the expression dynamics of LEF1 in skin during development and hair cycle. LEF1 expression in TSCs indicates that the LEF1/Wnt signal might help to establish a niche at the isthmus region for the lanceolate complex, the bulge stem cells and other neighboring cells.
GABI‐I: The global ant biodiversity informatics‐island database
Authors: Liu Cong , Economo Evan P. , Guénard Benoit 
Published: December 2022

AbstractIsland systems are known to harbor disproportionate amounts of geographically restricted biodiversity and to experience high rates of species loss, and they ultimately represent critical systems with significant conservation values. However, knowledge of the biodiversity value of insular systems remains highly fragmented and incomplete for many groups of organisms, especially insects. This gap limits our understanding of their global significance for biodiversity and inhibits prioritization for future exploration and conservation efforts. Here, we developed a new database to present current knowledge on ants on 2678 islands globally, based on nominal species, and provide information on their native or exotic status. In total, this database contains 7010 ant species (44.6% of the known global ant fauna) that have been recorded on islands globally from 449,232 records. In addition, this database identifies 108 large islands (area > 200 km2) that have received no ant sampling efforts globally. This new data set provides the most comprehensive understanding of ant diversity and composition on islands globally, opening up new opportunities to address questions on a multitude of research questions and fields related to biogeography, ecology, and evolution. This data set also provides a roadmap for future exploration and conservation actions in connection with ants on islands as well as overall ant diversity, with updates available as new records and taxonomic updates are published. There are no copyright restrictions on this database and users should cite this data paper in publications when using the data.
Natural products of relevance in the management of attention deficit hyperactivity disorder
Authors: Custódio Luísa , Vizetto‐Duarte Catarina , Cebeci Fatma , Özçelik Beraat , Sharopov Farukh , Gürer Eda Sönmez , Kumar Manoj , Iriti Marcello , Sharifi‐Rad Javad , Calina Daniela 
Published: December 2022

AbstractAttention deficit hyperactivity disorder (ADHD) is among the most common neurodevelopmental disorders characterized by motor hyperactivity, attention deficit, distraction, impulsive behavior, and relationship difficulties. It affects children, and thus over the years, there has been growing attention on the use of natural product formulations and their naturally‐occurring bioactive for overcoming the problem of ADHD. Concerning this issue, this review aims to discuss the ADHD treatment potential of natural products/formulations in vitro, in vivo as well as in clinical trials. To gather the most relevant data regarding bioactive compounds with potential therapeutic effects in the management of ADHD, the following databases were searched: PubMed/MedLine, ScienceDirect, and Google Scholar for articles in English. Various medicinal plants and their isolated biomolecules have been evaluated in vitro, in vivo, and in clinical trials for their ability to treat ADHD. Most of the analyzed research results showed an improvement of ADHD signs and symptoms in in vivo models, but the molecular mechanisms of action of the bioactive compounds are unclear and incompletely elucidated. Further studies are needed to determine the bioactive compound responsible for health‐promoting effects in ADHD. In addition, clinical studies should be performed over longer periods with more participants.
Katritzky Salts for the Synthesis of Unnatural Amino Acids and Late‐Stage Functionalization of Peptides
Authors: Yousif Ali Munaim , Colarusso Stefania , Bianchi Elisabetta 
Published: December 2022

AbstractPeptide drug discovery often benefits from the large structural diversity permitted by unnatural amino acids (UAAs). Indeed, numerous approved peptide drugs include UAAs in their sequences. Therefore, innovative chemical approaches either to synthesize UAAs or to allow late‐stage functionalization of peptides are emerging themes in peptide drug discovery. Thanks to the recent advances in deaminative strategies using alkylpyridiniums salts, often referred to as Katritzky salts, a variety of radical alkylation methods have been developed. In recent years the use of Katritzky salts have become popular in peptide chemistry due to their ease of preparation from a primary amine, which is a predominant functional group in amino acids. This review highlights the progress that has been made by using Katritzky salts in the synthesis of UAAs, late‐stage peptide functionalization, and peptide macrocyclization.