bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plastics shape the black soldier fly larvae gut microbiome and select for biodegrading functions
Authors: De Filippis Francesca , Bonelli Marco , Bruno Daniele , Sequino Giuseppina , Montali Aurora , Reguzzoni Marcella , Pasolli Edoardo , Savy Davide , Cangemi Silvana , Cozzolino Vincenza , Tettamanti Gianluca , Ercolini Danilo , Casartelli Morena , Caccia Silvia 
Published: September 2023

Abstract
Background
In the last few years, considerable attention has been focused on the plastic-degrading capability of insects and their gut microbiota in order to develop novel, effective, and green strategies for plastic waste management. Although many analyses based on 16S rRNA gene sequencing are available, an in-depth analysis of the insect gut microbiome to identify genes with plastic-degrading potential is still lacking.

Results
In the present work, we aim to fill this gap using Black Soldier Fly (BSF) as insect model. BSF larvae have proven capability to efficiently bioconvert a wide variety of organic wastes but, surprisingly, have never been considered for plastic degradation. BSF larvae were reared on two widely used plastic polymers and shotgun metagenomics was exploited to evaluate if and how plastic-containing diets affect composition and functions of the gut microbial community. The high-definition picture of the BSF gut microbiome gave access for the first time to the genomes of culturable and unculturable microorganisms in the gut of insects reared on plastics and revealed that (i) plastics significantly shaped bacterial composition at species and strain level, and (ii) functions that trigger the degradation of the polymer chains, i.e., DyP-type peroxidases, multicopper oxidases, and alkane monooxygenases, were highly enriched in the metagenomes upon exposure to plastics, consistently with the evidences obtained by scanning electron microscopy and 1H nuclear magnetic resonance analyses on plastics.

Conclusions
In addition to highlighting that the astonishing plasticity of the microbiota composition of BSF larvae is associated with functional shifts in the insect microbiome, the present work sets the stage for exploiting BSF larvae as “bioincubators” to isolate microbial strains and enzymes for the development of innovative plastic biodegradation strategies. However, most importantly, the larvae constitute a source of enzymes to be evolved and valorized by pioneering synthetic biology approaches.
Correlates of disordered eating and insulin restriction behavior and its association with psychological health in Taiwanese youths with diabetes mellitus
Authors: Chou Wei-Chih , Chou Yen-Yin , Pan Yu-Wen , Ou Tsung-Ying , Tsai Meng-Che 
Published: September 2023

AbstractBackgroundAdolescents and young adults (AYAs) with diabetes mellitus (DM) are prone to eating disorders that may worsen metabolic control. This study investigated the clinical and behavioral correlates of disordered eating and insulin restriction (DE/IR) behavior and its association with psychological health among AYAs with DM.MethodsWe enrolled patients with DM aged 10–30 years receiving insulin treatment in a tertiary medical center from 2019 to 2021. After obtaining informed consent, we assessed various visit-to-visit HbA1c measures indicating glycemic control, DE/IR behavior using the modified SCOFF questionnaire, weight-control practices (e.g., self-medication, induced vomiting, and over-exercising), and anxious and depressive symptoms using the Hospital Anxiety and Depression Scale. Correlation and hierarchical regression analyses were applied to understand the clinical and behavioral correlates of DE/IR behavior and its association with anxiety and depression.ResultsAmong the 110 patients with type 1 and type 2 DM recruited, we found 17.6% restricting insulin use and 6.3% self-medicating for weight control (higher in type 2 DM than type 1 DM). Hierarchical regression analyses showed HbA1c standard deviation (odds ratio = 2.18, [95% confidence interval 1.07–4.42]), body image (1.83, [1.05–3.20]), and dieting (4.74, [1.70–13.23]) associated with DE/IR behavior. Moreover, DE/IR behavior was further associated with anxiety (1.17 [1.08–1.27]) and depression (1.12 [1.03–1.22]).ConclusionDE/IR behavior is not uncommon among AYAs with DM, particularly those with type 2 DM, and may be associated with anxiety and depressive symptoms. In addition, HbA1c variability is correlated with DE/IR behavior, and the clinical implications need further exploration.
2‐year results of middle‐aged patients with two‐compartment cartilage lesions in one knee treated with two patient specific metal implants
Authors: Toom Daniel Aaron den , Rieke Markus , Elbadawi Allaeldin , Kösters Clemens ,   Maria‐Josef‐Hospital Greven
Published: September 2023

AbstractPurposeFocal chondral lesions of the femur are currently treated with biological repair or arthroplasty. However, some patients are not suitable for either one due to lesion size, age, or prior biological treatment attempts. While singular patient‐specific focal mini metal implants already showed good results, the outcomes of bicompartmental implantation of these implants have not been discussed in the literature yet. This study aims to evaluate clinical outcomes of patients who underwent bicompartmental implantation of two patient‐specific implants.MethodsThis prospective, non‐randomized, non‐comparative pilot study evaluates results up to two years after bicompartmental implantation of two implants (Episealer Implant, Episurf, Stockholm, Sweden).A damage report is compiled using a special MRI program and patient specific implants are manufactured, including 3D‐printed surgical instruments to provide exact placement of the implant.The patients were assessed repeatedly using the Knee Injury and Osteoarthritis Outcome Score (KOOS) and Visual Analogue Scale (VAS) for pain during the follow‐up.ResultsThe scores were evaluated three, 12, and 24 months after surgery and showed good results. The median in both scores improved from 37.7 for the KOOS5 preoperatively to 69.1 after 24 months and from 69 for the VAS for pain preoperatively to 9 after 24 months.ConclusionOverall, for the small study group presented, the early results are promising. With noticeable improvement in KOOS and VAS for pain after two years, patient specific implants appear to become relevant in future standardized treatment of femoral chondral lesions. Especially with bicompartmental implantation, full arthroplasty can be delayed even further.Level of EvidenceIV
A whole-body diffusion MRI normal atlas: development, evaluation and initial use
Authors: Sjöholm Therese , Tarai Sambit , Malmberg Filip , Strand Robin , Korenyushkin Alexander , Enblad Gunilla , Ahlström Håkan , Kullberg Joel 
Published: September 2023

Abstract
Background
Statistical atlases can provide population-based descriptions of healthy volunteers and/or patients and can be used for region- and voxel-based analysis. This work aims to develop whole-body diffusion atlases of healthy volunteers scanned at 1.5T and 3T. Further aims include evaluating the atlases by establishing whole-body Apparent Diffusion Coefficient (ADC) values of healthy tissues and including healthy tissue deviations in an automated tumour segmentation task.

Methods
Multi-station whole-body Diffusion Weighted Imaging (DWI) and water-fat Magnetic Resonance Imaging (MRI) of healthy volunteers (n = 45) were acquired at 1.5T (n = 38) and/or 3T (n = 29), with test-retest imaging for five subjects per scanner. Using deformable image registration, whole-body MRI data was registered and composed into normal atlases. Healthy tissue ADCmean was manually measured for ten tissues, with test-retest percentage Repeatability Coefficient (%RC), and effect of age, sex and scanner assessed. Voxel-wise whole-body analyses using the normal atlases were studied with ADC correlation analyses and an automated tumour segmentation task. For the latter, lymphoma patient MRI scans (n = 40) with and without information about healthy tissue deviations were entered into a 3D U-Net architecture.

Results
Sex- and Body Mass Index (BMI)-stratified whole-body high b-value DWI and ADC normal atlases were created at 1.5T and 3T. %RC of healthy tissue ADCmean varied depending on tissue assessed (4–48% at 1.5T, 6–70% at 3T). Scanner differences in ADCmean were visualised in Bland-Altman analyses of dually scanned subjects. Sex differences were measurable for liver, muscle and bone at 1.5T, and muscle at 3T. Volume of Interest (VOI)-based multiple linear regression, and voxel-based correlations in normal atlas space, showed that age and ADC were negatively associated for liver and bone at 1.5T, and positively associated with brain tissue at 1.5T and 3T. Adding voxel-wise information about healthy tissue deviations in an automated tumour segmentation task gave numerical improvements in the segmentation metrics Dice score, sensitivity and precision.

Conclusions
Whole-body DWI and ADC normal atlases were created at 1.5T and 3T, and applied in whole-body voxel-wise analyses.
Localizability estimation using correlation on occupancy grid maps
Authors: Kondo Maiku , Hoshi Masahiko , Hara Yoshitaka , Nakamura Sousuke 
Published: September 2023

AbstractIn the field of autonomous mobile robotics, reliable localization is important. However, there are real environments where localization fails. In this paper, we propose a method to estimate localizability based on occupancy grid maps. The localizability indicates the reliability of localization. There are several approaches to estimate localizability, we propose a method to estimate localizability as a covariance matrix of the Gaussian distribution using local map correlation. Our method can estimate the magnitude of the localization error and the characteristics of the error. To confirm the effectiveness of the proposed method, we constructed simulation environments that include representative shapes of indoor environments. We conducted an experiment to investigate the characteristics of the distribution of local map correlation. Furthermore, we also conducted an experiment of our method to estimate localizability on occupancy grid maps. The simulation experiment results showed that the proposed method could estimate the magnitude of the localization error and the characteristics of the error on occupancy grid maps. The proposed method was confirmed to be effective in estimating localizability.
Prevalence of hypertension among travelers and stability of blood pressure control during travel: a cross-sectional descriptive study and prospective cohort study
Authors: Gultawatvichai Watsapol , Matsee Wasin , Pisutsan Phimphan , Kusolsuk Teera , Silachamroon Udomsak , Mansanguan Chayasin , Lawpoolsri Saranath , Flaherty Gerard T. , Piyaphanee Watcharapong 
Published: September 2023

Abstract
Background
Hypertension is a common and important risk factor for cardiovascular disease which is the leading cause of death among the general population and travelers. Data on hypertension among travelers are very limited due to the scarcity of research reports in this specific population. Therefore, this study aimed to determine the prevalence of hypertension among adult travelers and the stability of blood pressure control during international trips using a mobile automated blood pressure device.

Methods
This was a cross-sectional descriptive study conducted at the Thai travel clinic, Hospital for Tropical Diseases in Bangkok, Thailand. All adult travelers completed a questionnaire which included demographic data, medical history, medication use, trip characteristics and hypertension awareness and knowledge. Standard two time blood pressure measurements were performed at the clinic to detect possible undiagnosed hypertension. Travelers with pre-existing hypertension were also invited to monitor their blood pressure level before and during their trip for a total of 14 days by using an automated blood pressure device and reporting the readings back to the study team.

Result
During July and October 2022, a total of 1,359 adult travelers visited the Thai Travel Clinic before their international trip. The overall prevalence of hypertension was 28.8%, including those with pre-existing hypertension (6.7%) and those with newly diagnosed hypertension (22.2%). Travelers with newly diagnosed hypertension were significantly younger than travelers with pre-existing hypertension (38.5 years vs. 55.6 years, p < 0.001). Eleven travelers agreed to monitor their blood pressure, Most (90.9%, 10/11) had stable blood pressure control during their trip. One participant had > 10 mmHg higher blood pressure during the trip, however this was not clinically significant. All participants remained well, and acute symptoms secondary to hypertension were not reported.

Conclusion
Up to 28.8% of adult travelers seen in pre-travel consultations had hypertension. Most of them were unaware of their blood pressure condition. Vital signs including blood pressure should be evaluated in all pre-travel visits in order to prevent undiagnosed severe hypertension that might lead to hypertensive crisis.
Co-creating active communities: processes and outcomes of linking public rehabilitation programs with civic engagement for active living in a Danish municipality
Authors: Hansen Anders Blædel Gottlieb , Hansen Marie Lønberg , Golubovic Sanja , Bloch Paul , Lorenzen Janne Kunchel , Almdal Thomas Peter , Ried-Larsen Mathias , Thorsen Ida Kær 
Published: September 2023

Abstract
Background
Increased levels of physical activity are associated with beneficial health effects for people with type 2 diabetes, cardiovascular disease and/or severe obesity; however, transforming knowledge about these effects into action is challenging. The aim of this paper is to explore lessons learnt from a co-creation process in a partnership project involving local stakeholders, including citizens, and researchers. The purpose of the process was to link a public health care institution with civil society organisations in the local community to make it possible for citizens to continue to be physically active after ending their public rehabilitation. Secondarily, this paper aims to develop a conceptual model of the above process.

Methods
The study constitutes the first part of Project Active Communities and was based on a partnership between three research institutions and a Danish rural municipality, involving municipal and civil society stakeholders and citizens with type 2 diabetes, cardiovascular disease and/or severe obesity in co-creation of concrete interventions for implementation. The co-creation process was divided into two tracks, one involving citizens (two workshops) and one involving municipal and civil society stakeholders (two workshops). The two tracks were concluded with a final workshop involving all stakeholders, including local politicians. Data sources are focus groups and bilateral meetings, workshop observations, and questionnaires.

Results
Lessons learnt include the importance of having a flexible timeframe for the co-creation process; giving room for disagreements and matching of mutual expectations between stakeholders; the value of a coordinator in the municipality to achieve acceptance of the project; and the significance of engaging local politicians in the co-creation process to accommodate internal political agendas. We have developed a conceptual model for a co-creation process, where we outline and explain three distinct phases: stakeholder identification and description, co-creation, and prototyping. The model can be adapted and applied to other sectors and settings.

Conclusions
This study documents lessons learnt in a co-creation process aiming to link a public health care institution with civil society organisations in the local community. Further, this study has specified productive co-creative processes and documented the various phases in a conceptual model.
A clinical retrospective study of implant as a risk factor for medication-related osteonecrosis of the jaw: surgery vs loading?
Authors: Kwon Yong-Dae , Jo Hyunmi , Kim Jae-Eun , Ohe Joo-Young 
Published: September 2023

Abstract
Background
Risk factors for developing medication-related osteonecrosis of the jaw (MRONJ) include the general condition of the patient, smoking habit, poor oral hygiene, and the type, duration, and administration route of the drug, dentoalveolar surgery, such as implant placement. This study aimed to discuss whether implants may induce osteonecrosis in older patients receiving long-term medication and to analyze the radiological pattern of the bone necrosis.

Methods
This study included 33 patients diagnosed with dental implant-associated medication-related osteonecrosis of the jaw. Data regarding the medical history, type of medication used, durations of administration, laboratory test results, onset of bone necrosis since implant placement, type of opposing teeth, and radiological pattern of the bone necrosis on cone-beam computed tomography were recorded in patients with and without implants.

Results
The most commonly used drug was bisphosphonate, with an average duration of use of 61.37 (± 53.72) months. The laboratory results showed average serum C-terminal cross-linking telopeptide (CTX) level of 0.23 ng/mL, vitamin D, 23.42 ng/mL, and osteocalcin, 4.92 ng/mL. Osteonecrosis occurred after an average of 51.03 (± 39.75) months following implant placement. Radiological evaluation revealed obvious sequestration in the implant-absent group, and the formation of a unit sequestration with an implant fixture (en bloc) was observed in the implant-present group. The patients underwent surgical treatment of sequestrectomy and explantation.

Conclusion
Implant placement, especially loading, may be considered a potential risk factor for the development of osteonecrosis in patients undergoing antiresorptive treatment.
Two-stage stochastic-robust model for the self-scheduling problem of an aggregator participating in energy and reserve markets
Authors: Wang Jian , Xie Ning , Huang Chunyi , Wang Yong 
Published: September 2023

AbstractThis paper addresses a two-stage stochastic-robust model for the day-ahead self-scheduling problem of an aggregator considering uncertainties. The aggregator, which integrates power and capacity of small-scale prosumers and flexible community-owned devices, trades electric energy in the day-ahead (DAM) and real-time energy markets (RTM), and trades reserve capacity and deployment in the reserve capacity (RCM) and reserve deployment markets (RDM). The ability of the aggregator providing reserve service is constrained by the regulations of reserve market rules, including minimum offer/bid size and minimum delivery duration. A combination approach of stochastic programming (SP) and robust optimization (RO) is used to model different kinds of uncertainties, including those of market price, power/demand and reserve deployment. The risk management of the aggregator is considered through conditional value at risk (CVaR) and fluctuation intervals of the uncertain parameters. Case studies numerically show the economic revenue and the energy-reserve schedule of the aggregator with participation in different markets, reserve regulations, and risk preferences.
Association between alcohol consumption and subclinical atrial fibrillation
Authors: Yu Ga-In , Kim Daehoon , Yu Hee Tae , Kim Tae-Hoon , Oh Il-Young , Park Jong Sung , Park Hyung-Seob , Park Junbeom , Lee Young Soo , Kang Ki-Woon , Shim Jaemin , Sung Jung-Hoon , Choi Eue-Keun , Joung Boyoung ,   The AF-Pacemaker Study Group
Published: September 2023

Abstract
Background
It has become important to identify and manage risk factors for subclinical atrial fibrillation (AF) with an increase in its detection rate. Thus, this research aimed to investigate whether alcohol consumption contributes to the development of subclinical AF.

Methods
This prospective study enrolled 467 patients without AF from a multicenter pacemaker registry. The incidence of subclinical AF (episodes of atrial rate > 220 beats per minute without symptoms) was compared between alcohol-drinking and non-drinking groups.

Results
During followup (median 18 months), the incidence and risk of long-duration atrial high-rate episodes (AHRE) ≥ 24 h were increased in the alcohol group compared to the non-alcohol group [5.47 vs. 2.10 per 100 person-years, adjusted hazard ratio (HR), 2.83; 95% confidence interval (CI), 1.14–7.04; P = 0.03]. After propensity score matching, the incidence and risk of long-duration AHRE were higher in the alcohol group (6.97 vs. 1.27 per 100 person-years, adjusted HR, 7.84; 95% CI, 1.21–50.93; P = 0.03). The mean burden of long-duration subclinical AF was higher in the alcohol group than in the non-alcohol group (0.18 vs. 1.61% during follow-up, P = 0.08).

Conclusion
Alcohol consumption was associated with an increased risk of subclinical AF. Long-duration AHRE incidence and AHRE burden were higher in alcohol drinkers than in non-drinkers.
Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
Authors: Ohnishi Chie , Ohnishi Takashi , Ntiamoah Peter , Ross Dara S. , Yamaguchi Masahiro , Yagi Yukako 
Published: September 2023

AbstractIn the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for calibrating the color variation due to the scanner. The results of the automated HER2 assessment were compared to confirm the effectiveness of two calibration slides. The IHC-CS and HER2 breast cancer cases were stained on different days. All stained slides and CCS were digitized by two different WSI scanners. Results revealed 100% concordance between automated evaluation and the pathologist’s assessment with both the scanner and staining calibration. The proposed method may enable consistent evaluation of HER2.
Designing, assessing, and effectiveness a psychological interventions program with a robot for children sexual care
Authors: Tahan Mohammad , Afrooz Gholamali , Bolhari Jafar 
Published: September 2023

Abstract
Background
Child sexual abuse has deep and profound effects on personality and growth of children so that it is recognized as one of the major health issues in different countries. A psychological interventions program with a robot was designed and its effects on children sexual care was examined.

Results
The results showed that experts confirmed and supported 85% of the total content of the program and the sessions, and the program was effective in improving sexual cares in the subjects (p < 0.01).

Conclusion
The program to protect children against sexual abuse through empowering them by the skills to protect themselves, recognize good and bad physical touches, and find a trustable adult can be considered as a therapeutic method by consultants, psychologists, and authorities.