bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel TPD52L2-ROS1 gene fusion expanding the molecular alterations in inflammatory myofibroblastic tumor: case report and literature review
Authors: Liu Xuguang , Duan Yaqi , Wang Guoping , Zhu Pengcheng 
Published: September 2023

Abstract
Background
Inflammatory myofibroblastic tumor (IMT) is a distinctive tumor composed of spindle cells accompanied by mixed inflammatory cells, and immunohistochemical positivity for ALK (anaplastic lymphoma kinase protein) can be detected in half of IMTs. The diagnosis of ALK-negative IMT could be a challenge. Recently, the fusions of some kinase genes, such as RET, NTRK1, ROS1, etc., are revealed in ALK-negative IMT.

Case presentation
A 19-year-old woman presented with swelling of the left upper arm. Magnetic resonance imaging (MRI) scan revealed a tumor in the left postbrachium extended to the left axillary, serratus anterior muscle, and latissimus dorsi muscle. Histopathologically, the irregular-circumscribed tumor was composed of dense spindle-shaped cells with eosinophilic abundant cytoplasm and hyalinized mesenchyme in an inflammatory background. Immunohistochemically (IHC), tumor cells were positive for SMA, MDM2, and p16; the cells were negative for desmin, MyoD1, Myogenin, pan-cytokeratin, S100, SOX10, HMB45, Malen-A, CD34, CD31, CD99, and ALK. By RNA-based NGS, a novel fusion between TPD52L2 3’ end of exon 1–4 and ROS1 5’ end of exon 36–43 was revealed. ROS1 IHC staining was negative. The final diagnosis of IMT with TPD52L2-ROS1 fusion was made. Subsequently, the patient experienced a good clinical response to Crizotinib, and clinical follow-up showed stable disease after 9 months.

Conclusion
This report expands the spectrum of ROS1 gene rearrangements in the IMT and highlights the importance of molecular analysis of IMT for getting a diagnostic clue and determining potential therapeutic strategies.
Implementation of coordinated spontaneous awakening and breathing trials using telehealth-enabled, real-time audit and feedback for clinician adherence (TEACH): a type II hybrid effectiveness-implementation cluster-randomized trial
Authors: Grissom Colin K. , Holubkov Richard , Carpenter Lori , Hanna Bridgett , Jacobs Jason R. , Jones Christopher , Knighton Andrew J. , Leither Lindsay , Lisonbee Dee , Peltan Ithan D. , Winberg Carrie , Wolfe Doug , Srivastava Rajendu 
Published: September 2023

Abstract
Background
Intensive care unit (ICU) patients on mechanical ventilation often require sedation and analgesia to improve comfort and decrease pain. Prolonged sedation and analgesia, however, may increase time on mechanical ventilation, risk for ventilator associated pneumonia, and delirium. Coordinated interruptions in sedation [spontaneous awakening trials (SATs)] and spontaneous breathing trials (SBTs) increase ventilator-free days and improve mortality. Coordination of SATs and SBTs is difficult with substantial implementation barriers due to difficult-to-execute sequencing between nurses and respiratory therapists. Telehealth-enabled remote care has the potential to overcome these barriers and improve coordinated SAT and SBT adherence by enabling proactive high-risk patient monitoring, surveillance, and real-time assistance to frontline ICU teams.

Methods
The telehealth-enabled, real-time audit and feedback for clinician adherence (TEACH) study will determine whether adding a telehealth augmented real-time audit and feedback to a usual supervisor-led audit and feedback intervention will yield higher coordinated SAT and SBT adherence and more ventilator-free days in mechanically ventilated patients than a usual supervisor-led audit and feedback intervention alone in a type II hybrid effectiveness-implementation cluster-randomized clinical trial in 12 Intermountain Health hospitals with 15 ICUs. In the active comparator control group (six hospitals), the only intervention is the usual supervisor-led audit and feedback implementation. The telehealth-enabled support (TEACH) intervention in six hospitals adds real-time identification of patients eligible for a coordinated SAT and SBT and consultative input from telehealth respiratory therapists, nurses, and physicians to the bedside clinicians to promote adherence including real-time assistance with execution. All intubated and mechanically ventilated patients ≥ 16 years of age are eligible for enrollment except for patients who die on the day of intubation or have preexisting brain death. Based on preliminary power analyses, we plan a 36-month intervention period that includes a 90-day run-in period. Estimated enrollment in the final analysis is up to 9900 mechanically ventilated patients over 33 months.

Discussion
The TEACH study will enhance implementation science by providing insight into how a telehealth intervention augmenting a usual audit and feedback implementation may improve adherence to coordinated SAT and SBT and increase ventilator-free days.

Trial registration
Clinicaltrials.gov, NCT05141396, registered 12/02/2021.
Impact of multimodal analgesia on postoperative anxiety and depression following total knee arthroplasty
Authors: Zheng Changjian , Hou Zijun , Wang Tingting , Yi Lingmei , Chen Yongquan 
Published: September 2023

Abstract
Background
Postoperative pain after total knee arthroplasty (TKA) can cause negative emotions, such as anxiety and depression, which can severely affect a patient’s long-term quality of life.

Objective
This study aimed to investigate the impact of multimodal analgesia (MMA) on postoperative anxiety and depression following total knee arthroplasty.

Methods
This study included 161 patients who underwent TKA from October 2020 to October 2022 in the First Affiliated Hospital of Wannan Medical College, including 79 cases in the control group and 82 cases in the multimodal analgesia group (MMA). The MMA group were administered acetaminophen 0.5 g/d orally 3 days before the surgery, and an ultrasound-guided fascia iliac compartment block (FICB) with 0.25% ropivacaine 30 ml in the inguinal region ipsilateral to the surgery was performed 1 h before surgery. After the surgery, 100 ml solution includes 100 mg ropivacaine, 2.5 mg morphine, and 0.25 mg epinephrine for intra-articular and periarticular injection. Postoperative conventional intravenous analgesia was used in the control group, including 100 mg ropivacaine, 2.5 mg morphine, and 0.25 mg epinephrine for intra-articular and periarticular injection. Patients were scored for pain, anxiety, and depression in the ward at 3 and 7 days postoperatively, and postoperative patients were scored using telephone callbacks at 3 months postoperatively.

Results
It was found that the visual analog scale (VAS) scores for pain at rest at 3 days, 7 days, and 3 months postoperatively were significantly lower in the MMA group than in the control group (P < 0.05). The scores for pain with movement were significantly lower in the MMA group than in the control group at 3 days and 7 days postoperatively (P < 0.01), but they were similar at 3 months postoperatively. Compared to the control group, the MMA group had significantly higher American Knee Society scores (AKS) at 3 days, 7 days, and 3 months postoperatively (P < 0.05). Compared to the control group, the MMA group had significantly higher Lower Extremity Functional Scale and Hospital Anxiety and Depression Scale scores (HADS) (P < 0.05) at 3 days and 7 days postoperatively; compared to the control group, the MMA group had a significantly shorter hospital stay (P < 0.01).

Conclusion
Multimodal analgesia can alleviate postoperative anxiety and depression in the short term, reduce perioperative pain, improve postoperative recovery, and shorten the length of hospital stay.
Pulmonary and clinical outcomes of patients with severe rigid scoliosis and type I respiratory failure treated with halo-pelvic distraction
Authors: Zhao Deng , Wang Fei , Hu Zhengjun , Zhong Rui , Liang Yijian 
Published: September 2023

Abstract
Background
The severe rigid scoliosis patients with type I respiratory failure could not tolerate complicated corrective surgery. Preoperative halo-pelvic distraction (HPD) is used to reduce the curve magnitude and improve the pulmonary function before surgery. The present study aimed to retrospectively analyze the pulmonary and clinical outcomes of preoperative HPD in severe rigid spinal deformity with type I respiratory failure.

Methods
Eighteen cases of severe rigid scoliosis and type I respiratory failure treated with preoperative HPD and corrective surgery for spinal deformity between 2016 and 2018 were retrospectively reviewed. Patient demographics, major coronal curve and kyphosis, correction rates, heights, pulmonary function, distraction time, and postoperative neurological complications were recorded for all cases.

Results
The averaged duration of distraction was 9.1 ± 2.3 months. The coronal curve was corrected from 168° ± 14° to 58° ± 11° at the end of HPD. The kyphosis curve reduced from 151° ± 29° to 65° ± 10°. Meanwhile, the mean stand body height increased by 23.9 ± 5.3 cm. Significantly increased mean FVC (1.52 ± 0.43 L vs. 0.95 ± 0.44 L) and improved percent-predicted values for FVC (37 ± 10% vs. 23 ± 9%) were observed after HPD. The pressure of oxygen (PaO2) increased from 54.5 ± 2.0 to 84.8 ± 4.7 mmHg. Scoliosis and kyphosis curve, respectively, averaged 48 ± 8°and 30 ± 14° after final fusion and instrumentation, with a mean correction of 71% and 80%, respectively. No severe complication occurred during the distraction.

Conclusions
HPD may be useful for severe rigid scoliosis patients with type I respiratory failure. Pulmonary functions in patients with severe rigid scoliosis can be significantly improved by HPD. They are then better able to tolerate complicated corrective surgery.
Effectiveness of neuromuscular electrical stimulation in severe acute pancreatitis complicated patients with acute respiratory distress syndrome: study protocol for a randomized controlled trial
Authors: Zhou Feng , Fan Dingrong , Feng Yan , Zhou Cuijuan , Chen Xiaodong , Ran Xiaoyun , Tan Botao 
Published: September 2023

Abstract
Background
Severe acute pancreatitis complicated by acute respiratory distress is a common cause of intensive care unit (ICU) admission. These patients are at risk of a decline in physical activity due to bed rest. Neuromuscular electrical stimulation (NMES) has been recommended for ICU patients to strengthen muscles, but its effects on muscle atrophy, respiratory function, multiple organ dysfunction, and functional status of these patients remain to be proven.

Methods
Patients (n = 80) will be prospectively randomized into an NMES group and a control group. The NMES group will receive NMES for 1 h per day for 7 days, and both the control and NMES groups will receive usual care. The efficacy will be assessed by an experienced physiotherapist and sonographer who will be blinded to the patient’s group assignment. Muscle power assessment (MRC scale), lower extremity circumference, grip strength, activities of daily living (Barthel index), and Marshall scores will be measured at baseline and posttreatment. The functions of the diaphragm assessments will be measured daily. Barthel index measurements will be followed up in the 1st month, 3rd month, and 6th month after discharge.

Discussion
The trial will explore the effectiveness of NMES in functional status and diaphragm function in patients with SAP complicated with ARDS. The results of this trial will provide strong evidence of the efficacy of NMES in treating SAP patients with ARDS.

Trial registration
This trial has been registered at the Chinese Clinical Trial Registry, and the registry name is “Effectiveness of neuromuscular electrical stimulation in severe acute pancreatitis complicated patients with acute respiratory distress syndrome: study protocol for a randomized controlled trial,” URL: https://www.chictr.org.cn, numbered ChiCTR2300068995. Date of Registration: 2023-03-03.
Loop-mediated isothermal amplification identifies nematode Leidynema in the hindgut of non-pest cockroach
Authors: Schnell Laura J , Khan Faraz , Hart Mel , Davis Maria C 
Published: September 2023

AbstractCockroach microbiome studies generally focus on pest cockroach species belonging to the Blattidae and Ectobiidae families. There are no reports characterizing the gut microbiome of non-pest cockroach species Blaberus discoidalis (family Blaberidae), which is commonly used as a food source for insectivorous animals. We discovered the parasitic nematode Leidynema appendiculata in the B. discoidalis hindgut during initial work characterizing the gut microbiome of this organism. To determine the proportion of the B. discoidalis colony that was colonized by L. appendiculata, 28 S rDNA was amplified using two Methods: endpoint polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). B. discoidalis colonies were raised on three diet types (control, high fibre, and high fat and salt) for 21 days before dissection. Each individual was sexed during dissection to identify potential sex-based effects of colonization. Data collected were analysed to determine if diet and sex impacted parasite colonization patterns. LAMP detected a higher proportion of parasite positive samples when compared to endpoint PCR. No sex- or diet-based differences in L. appendiculata colonization were found. This study adds to the limited existing knowledge of the B. discoidalis gut microbiome.
A polo ball in the right atrium, importance of echocardiographic characteristics of intracardiac myxomas: a case report
Authors: Hamdan Mira , Alam Boutros , Kossaify Antoine 
Published: September 2023

Abstract
Background
Myxomas are the most prevalent type of heart tumors, mainly occurring in the left atrium, with approximately 20% of cases found in the right atrium. Timely diagnosis and appropriate management of myxomas are crucial for favorable outcomes and to minimize complications.

Case presentation
A 77-year-old Asian male with no significant medical history presented with intermittent trepopnea and palpitations. Physical examination revealed regular heart sounds and no other relevant findings. A transthoracic echocardiogram showed a large, round-shaped, smooth-edged mass with diameter of 86 mm, occupying most of the right atrium. Mild tricuspid flow obstruction and mild left ventricular systolic dysfunction were also observed. Cardiac tomography confirmed the size, smooth edges, and showed microcalcifications of the mass, with no invasion of surrounding tissues. Surgical intervention successfully removed the spheroid mass, leading to the alleviation of symptoms. Histopathology confirmed the myxoma nature of the mass. A comprehensive discussion based on relevant medical literature is provided, with emphasis on echocardiographic characteristics of the mass with relation to potential embolic disease.

Conclusion
This case shows an atypical presentation of an exceptionally large myxoma in the right atrium, resembling the size of a polo ball, in a patient presenting with supraventricular arrhythmia and trepopnea. Early diagnosis and appropriate management played a vital role in achieving a successful outcome for the patient.
Imaging of fibrogenesis in the liver by [18F]TZ-Z09591, an Affibody molecule targeting platelet derived growth factor receptor β
Authors: Wegrzyniak Olivia , Zhang Bo , Rokka Johanna , Rosestedt Maria , Mitran Bogdan , Cheung Pierre , Puuvuori Emmi , Ingvast Sofie , Persson Jonas , Nordström Helena , Löfblom John , Pontén Fredrik , Frejd Fredrik Y. , Korsgren Olle , Eriksson Jonas , Eriksson Olof 
Published: September 2023

Abstract
Background
Platelet-derived growth factor receptor beta (PDGFRβ) is a receptor overexpressed on activated hepatic stellate cells (aHSCs). Positron emission tomography (PET) imaging of PDGFRβ could potentially allow the quantification of fibrogenesis in fibrotic livers. This study aims to evaluate a fluorine-18 radiolabeled Affibody molecule ([18F]TZ-Z09591) as a PET tracer for imaging liver fibrogenesis.

Results
In vitro specificity studies demonstrated that the trans-Cyclooctenes (TCO) conjugated Z09591 Affibody molecule had a picomolar affinity for human PDGFRβ. Biodistribution performed on healthy rats showed rapid clearance of [18F]TZ-Z09591 through the kidneys and low liver background uptake. Autoradiography (ARG) studies on fibrotic livers from mice or humans correlated with histopathology results. Ex vivo biodistribution and ARG revealed that [18F]TZ-Z09591 binding in the liver was increased in fibrotic livers (p = 0.02) and corresponded to binding in fibrotic scars.

Conclusions
Our study highlights [18F]TZ-Z09591 as a specific tracer for fibrogenic cells in the fibrotic liver, thus offering the potential to assess fibrogenesis clearly.

Graphical abstract
A fault segment location method for distribution networks based on spiking neural P systems and Bayesian estimation
Authors: Wang Yi , Wang Tao , Liu Liyuan 
Published: September 2023

AbstractWith the increasing scale of distribution networks and the mass access of distributed generation, traditional centralized fault location methods can no longer meet the performance requirements of speed and high accuracy. Therefore, this paper proposes a fault segment location method based on spiking neural P systems and Bayesian estimation for distribution networks with distributed generation. First, the distribution network system topology is decoupled into single-branch networks. A spiking neural P system with excitatory and inhibitory synapses is then proposed to model the suspected faulty segment, and its matrix reasoning algorithm is executed to obtain a preliminary set of location results. Finally, the Bayesian estimation and contradiction principle are applied to verify and correct the initial results to obtain the final location results. Simulation results based on the IEEE 33-node system validate the feasibility and effectiveness of the proposed method.
Pre-emptive ultrasound-guided superior hypogastric plexus block in pelvic cancer surgeries: a randomized double-blinded study
Authors: Mahran Essam , Wadod Mohamed A. 
Published: September 2023

Abstract
Background
Superior hypogastric plexus is a retroperitoneal plexus that receives visceral sensation from pelvic viscera. Superior hypogastric plexus block (SHPB) was used for chronic pelvic pain and recently studied for postoperative pain. We examined the safety and efficacy of preemptive anterior US-guided SHPB to reduce postoperative morphine consumption. Thirty-six patients undergoing pelvic cancer surgery were randomly divided into two equal groups; group S in which patients received anterior US-guided SHPB immediately after induction of general anesthesia and before skin incision using 20 ml bubivacaine 0.5%, group C control group in which 20 ml normal saline was given by the same technique. Patients of both groups received morphine via PCA postoperative and followed for 24 h. In both groups, we measured the total morphine consumption, VAS, vital signs, and side effects.

Results
Demographic data, duration, and type of surgery were comparable in both groups. Total 24 h morphine consumption in mg was significantly lower in group S (43.8 ± 2) than in group C (54.83 ± 2) with P value < 0.001. VAS was significantly lower in group S in all time intervals from 2 till 24 h postoperative. Side effects were minimal with no significant difference between both groups.

Conclusion
Preemptive US-guided SHPB is a relatively safe and effective method to reduce postoperative opioid demands after pelvic cancer surgeries.

Trial registration
ClinicalTrials.gov NCT04732234 in 1–2-2021.
Assessing genetic variation in Gossypium barbadense L. germplasm based on fibre characters
Authors: Alagarsamy Manivannan 
Published: September 2023

Abstract
Background
Gossypium barbadense L. has specific fibre in terms of its length, strength, and fineness, and known as extra-long staple (ELS) cotton, Sea–Island cotton, or Egyptian cotton. Narrow genetic base with less genetic variability is observed in G. barbadense germplasm. Hence, this study was aimed to evaluate the genetic variability present in 108 germplasm accessions of G. barbadense and to identify the superior genotypes based on the fibre traits.

Results
We evaluated 108 accessions for five fibre quality traits along with three checks in augmented block design. All fibre traits showed significant differences among genotypes, indicating that there is genetic potential for improvement. Fibre strength and micronaire (MIC) showed high phenotypic and genotypic coefficients of variation. High heritability combined with high genetic advance as percentage of mean (GAM) was recorded for fibre length, strength, and micronaire. Fibre strength and fibre length were significantly correlated with each other, while both showed negative correlation with micronaire. Principal component analysis and Biplot analysis showed that uniformity index discriminated all the genotypes in higher level, while fibre length and strength were medium in discrimination power. Biplot revealed genotypes DB 16, EC959191, GSB 39, ARBB 20, 5746U, EA 203, and EA 201 were genetically diverse. Hierarchal cluster analysis based on unweighted paired group method using arithmetic average (UPGMA) grouped the genotypes into four clusters, with each cluster consisting of 4, 18, 48, and 38 genotypes, respectively.

Conclusion
Among the genotypes, 34 for fibre length (> 35 mm), 18 for fibre strength (> 40.4 g·tex−1) and 66 for micronaire (3.7-4.2, A grade) were identified as potential accessions based on their superiority. The superior fibre genotypes identified in this study are potential lines for the ELS cotton breeding program.
Nature can cool cities, but proceed with caution
Authors: Mabon Leslie , Connor Ben , Moncaster Alice , Pearce Catherine , Pratt Eleanor , Shih Wan-Yu , Tsai Meng-Chin , Vseteckova Jitka , Waights Verina , Wolstenholme Ruth 
Published: September 2023

AbstractIncreased extreme heat events draw attention to the potential of urban nature as a heat adaptation strategy for cities. This is reflected in multiple scientific perspective pieces, policy documents and science media publications advocating for urban greening as a cooling approach. Although attention to the dangers of heat and the benefits of urban nature is welcomed, it is vital that nature-based approaches to cooling are underpinned by diverse knowledge and a sound understanding of what nature in cities can and cannot do. We explain why an evidence-driven and cautious approach to heat adaptation through urban greening is so important, and propose three actions that urban actors can take towards effective and equitable long-term cooling through urban nature: enabling dialogue between different sectors with multiple remits; including diverse knowledge systems in planning and governance processes; and investing in long-term stewardship for the climatological and societal conditions of the coming decades.Policy and practice recommendation• Create fora for dialogue between governments, residents, civil society and developers from planning stage for green cooling;• Cooling through nature must be driven by expertise spanning diverse knowledge systems, combined with local knowledge and community needs;• Consider future climates and stewardship when planning urban cooling via nature.Science highlights• Understanding link between urban thermal environment and nature is an inter- and transdisciplinary task;• Critical need for evidence of how greening reduces heat impacts across different social and cultural contexts;• Evidence of how species perform under future climates required for stewardship of urban nature.