bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meta-analysis of Genetic polymorphism of Enhancer of Zeste Homolog2 gene in cancer susceptibility
Authors: Gautam Nisha , Kaur Mandeep , Kashyap Surender 
Published: September 2023

ABSTRACT
The alteration in the expression of enhancer of zeste homolog-2 (EZH2) gene is very well known in the progression, severity, and aggressiveness of cancer. Hence, it is important to study the genomic variation of the EZH2 gene. Previously, many association-based studies investigated the association between rs2302427C>G and cancer susceptibility. However, the result had been inconsistent. Therefore, our meta-analysis aimed to identify the association between EZH2 rs2302427 and cancer risk. A systematic literature search was done for databases PubMed, Google Scholar, Science Direct, and Cochrane library up to September 2020 and statistical analysis was performed by RevMan v 5.3. A total of six studies comprised 1876 cases and 2555 controls were included in the current meta-analysis. The pooled analysis showed that overall, there is significant association of rs2302427 C>G change with reduced cancer risk (odds ratio = 0.60, 95% confidence interval [0.35–1.03], P = 0.07) but non-significantly. Further, the subgroup analysis also revealed that there is no significant difference between the Asian and European population, and both exhibit the protective nature of rs2302427 with cancer. The present meta-analysis indicated that EZH2 rs2302427 has an association with cancer in reducing the risk but for the Indian population studies are required as the Indian population comprises various sub-population genetically isolated for long.
Review of clinical applications and challenges with surface-guided radiation therapy
Authors: Bellala Ravishankar , Kuppusamy Anandakrishnan , Bellala Venkata Madhavi , Tyagi Tulika , Manoharan Surendhiran , Gangarapu Gunasekhar , Bellala Rishik 
Published: September 2023

ABSTRACT

Aim:
To evaluate the use of this new technique, surface-guided radiotherapy (SGRT), for patient setup and motion management in various cancers.

Materials and Methods:
Data was collected from 533 patients, who received treatment in our hospital for various malignancies using SGRT from October 2019 to April 2021. We studied patient setup, interfraction position, and patient position during the breath-hold (BH) technique. The main advantage of SGRT is that, it is completely non-invasive and uses visible light to compare the patient’s skin surface in the treatment room and planned treatment position. In this analysis, Monaco 5.51.10 (Elekta) treatment planning system, Versa HD Linear Accelerator, and AlignRT 6.2 (Vision RT) SGRT system were used.

Results:
With SGRT, treatment setup time can be reduced with more precision and techniques like Deep inspiration breathhold (DIBH) can be done with very good compliance.

Conclusion:
SGRT has shown improved accuracy in patient setup compared to conventional laser setup. The daily kilo voltage imaging frequency can be reduced; it helps in reducing additional radiation exposure due to imaging. SGRT has demonstrated reproducibility with adequate accuracy in BH treatments in DIBH for breast and SBRT.
Metaplastic breast carcinoma: Analysis of clinical and pathologic features, a five-year study
Authors: Chaudhary Dimple , Balhara Kirti , Mandal Shramana , Mallya Varuna , Tomar Reena , Khurana Nita , Bains Lovnish 
Published: September 2023

ABSTRACT

Background:
Metaplastic carcinoma breast (MCB) is a rare tumor comprising of both glandular and non-glandular patterns with epithelial and or mesenchymal components. Due to their varied clinicomorphological features, diagnosis has been challenging.

Aim:
To study the clinicopathological and histomorphology of cases of metaplastic carcinoma breast diagnosed in a tertiary care hospital along with literature review.

Materials and Methods:
This is a retrospective study including data of 11 patients who were diagnosed with MCB either on trucut or mastectomy specimens conducted between January 2014 and December 2018.

Results:
The study includes 11 patients, out of which 10 were diagnosed on mastectomy while one on trucut specimen. All the patients were women with the mean age of presentation being 43.8 years. The most common presentation was palpable breast lump with mean tumor size of 7.3 cm in greatest dimension. Skin involvement was seen in 36.3% of cases. While eight cases (72.7%) were classified as epithelial, three (27.2%) were classified as mixed. Amongst epithelial variety, in eight cases, squamous component was seen along with infiltrating ductal carcinoma (IDC) while one was pure squamous type. In mixed variety, one case showed spindled areas along with squamous areas and areas of IDC. Other two showed focal sarcomatous and cartilaginous areas in one and angiosarcomatous, bone and cartilage formation admixed with areas of IDC in other case.

Conclusion:
MCB are rare breast tumors with aggressive course and are characterized by their large size and rapid growth rate. Recently, there has been an upsurge in the cases of MCB due to increase in recognition of this entity. It has to be distinguished from other tumors by the pathologists so as to guide proper treatment.
The XRCC1 and TP53 gene polymorphisms are associated with advanced-stage disease and early distant metastasis at diagnosis in non-small cell lung cancer
Authors: Karaağaç Mustafa , Geredeli Çağlayan , Yıldırım Mahmut Selman , Altınok Tamer , Dede İsa , İnal Ali , Zamani Ayşe Gül , Kaya Buğra , Demirkazık Ahmet , Bozcuk Hakan , Artaç Mehmet 
Published: September 2023

ABSTRACT

Background:
Studies on single nucleotide polymorphisms (SNPs) in non-small cell lung cancer (NSCLC) suggest that DNA repair capacity may have prognostic implications for disease recurrence and survival. However, there is no study investigating the relationship between SNPs and the risk of metastasis at the time of initial diagnosis in patients with NSCLC.

Objective:
This study aimed to investigate the potential predictive value of SNPs in detecting the risk of metastasis at the time of initial diagnosis and poor prognosis in patients with NSCLC.

Material and Methods:
In this prospective cohort study, we evaluated 275 patients with NSCLC. Analysis of SNPs from peripheral blood cells was performed by a polymerase chain reaction. Excision repair cross-complementing group 1 (ERCC1)- Asn118Asn, excision repair cross-complementing group 2 (ERCC2)-Lys751Gln, X-ray repair cross-complementing group 1 (XRCC1)-Arg399Gln, and tumor protein 53 (TP53)-Arg72Pro polymorphisms were evaluated in conjunction with the development of metastasis.

Results:
The ERCC1 normal genotype, ERCC2 heterozygote genotype, XRCC1 normal genotype, and TP53 normal genotype were associated with a higher stage and more advanced-stage disease at the time of initial diagnosis (P = 0.027, 0.005, <0.001, and 0.006, respectively). Also, XRCC1 normal genotype and TP53 normal genotype were associated with the risk of metastasis at the time of initial diagnosis (P = <0.001 and 0.002, respectively). Moreover, the XRCC1 normal genotype was associated with the risk of brain metastasis at the time of initial diagnosis (P = 0.031).

Conclusions:
We showed that SNPs are related to a higher stage and more advanced-stage disease at the time of initial diagnosis in patients with NSCLC, and XRCC1 and TP53 gene polymorphisms are associated with the risk of metastasis. These results may contribute to the identification of high-risk groups and may help to earlier diagnosis and treatment in patients with NSCLC.
Local therapy in advanced melanoma after immune checkpoint inhibitors aiming to achieve complete response
Authors: de Barros e Silva Milton José , Teixeira Marcos Rezende , Lobo Matheus de Melo , Molina André Sapata , Bertolli Eduardo , Filho Ivan Dunshee de Abranches Oliveira Santos , Ribeiro Heber Salvador Castro , Pelizon Antônio Cássio de Assis , Pinto Clóvis Antônio Lopes , Neto João Pedreira Duprat 
Published: September 2023

ABSTRACT

Background and Objectives:
New scenarios for local therapy have arisen after starting immune checkpoint inhibitors (ICIs) to treat advanced melanoma (AM). The aim of this study is to examine the role of local therapies with curative intention for patients with AM that have been on ICI.

Methods:
This was a single institution, retrospective analysis of unresectable stage III or IV melanoma patients on treatment with anti-PD1 ± anti-CTLA-4 who underwent local therapy with curative intention with no other remaining sites of disease (NRD).

Results:
Of the 170 patients treated with ICI, 19 (11.2%) met the criteria of curative intention. The median time on ICI before local therapy was 16.6 months (range: 0.92–43.2). At the time of the local treatment, the disease was controlled in 16 (84.25%) and progressing in 3 patients (15.75%); 14 patients (73.7%) treated a single lesion and 5 (26.3%) treated 2 to 3 lesions. In a median follow-up of 17 months (range: 1.51–38.2) after the local therapy and 9.8 months after the last ICI cycle (range: 0.56–31), only 2 (10.5%) out of 19 patients relapsed.

Conclusions:
Patients with AM on treatment with ICI were able to achieve NRD after local treatment and may benefit from long-term disease control without systemic treatment.
A study on the anticancer activity of imidazolyl benzamide derivative-IMUEB on a 549 lung cancer cell line
Authors: Bhat Suhail Ahmad , Pajaniradje Sankar , Bhunia Sayandeep , Subramanian Srividya , Chandramohan Sathyapriya , Parthasarathi D , Bhaskaran Savitha , Padushah Syed Ali M , Rajagopalan Rukkumani 
Published: September 2023

ABSTRACT

Background:
Cancer is a deadly disease, which is due to the uncontrolled division of cells with abnormal or unusual characteristics. It is a consequence of lethal mutations occurring due to various chemical and physical carcinogens, affecting many cellular signalling pathways and leading to uncontrolled proliferation. In this study, we analyzed the effect of 4-(1H-imidazol-1-yl)-N-(2-(3-(4-methylbenzyl) ureido) ethyl)benzamide (IMUEB), an imidazole derivative, on A549 cells (lung cancer cells).

Methods:
The MTT and LDH assays were performed to measure the cytotoxicity of IMUEB against A549 cells. Apoptotic mode of cell death of A549 cells was determined by fluorescence imaging by using different stains. Flow cytometry was performed to detect the cell cycle arrest. Western blotting was performed to determine the levels of apoptotic protein. Wound healing assay was performed to find the effect of IMUEB on cell migration. In silico molecular docking of IMUEB was performed to predict its affinity towards apoptotic proteins and metastasis related enzymes.

Result and Discussion:
The MTT assay showed an increase in cytotoxicity with increasing concentrations of IMUEB. In addition, it was found that IMUEB arrests cell cycle at G1 phase as detected by flow cytometry analysis and induces apoptosis. The treatment with IMUEB drastically decreased the migratory potential of A549 cells as evaluated by migration and invasion assay. By Western blotting analysis, it was found that the concentration of caspase-3 was increased after the treatment with IMUEB.

Conclusion:
Altogether, our results indicate that IMUEB shows antitumor activity by inhibiting proliferation and inducing apoptosis in A549 cells.
Life after total skin electron irradiation; A perspective through the eyes of a radiation oncologist
Authors: Parida Dillip K. , Barik Sandip K. 
Published: September 2023

ABSTRACT
Mycosis fungoides (MF) remains a challenge as a disease from its diagnosis through treatment and follow-up. The rarity of the disease and uncharacteristic clinical manifestations pose difficulty in diagnosis, and the lack of treatment facilities adds to the management woes. Though the Stanford technique is the most accepted modality of total skin electron beam therapy (TSEBT), the implementation details are still unstandardized. Different centers adopt different methodologies as per their convenience and suitability. We present a patient of MF with many dimensions of prediagnosis clinical features to the diagnosis, treatment, and follow-up with subsequent developments over a period of 24 years that may help to understand the disease and management in a better manner.
A Dosimetric Comparison of Volumetric-modulated Arc Therapy and IMRT for Cochlea-sparing Radiation Therapy in Locally Advanced Nasopharyngeal Cancer
Authors: Nanda Siddhartha , Parida Sourajit , Ahirwar Manish Kumar 
Published: September 2023

Background:
Head-and-neck cancer treatment includes radiotherapy as a crucial component. However, radiotherapy, like other treatment modalities, has its own side effects, some of which can be avoided using the latest medical technology and understanding the illness. Despite being a relatively uncommon subtype of head-and-neck cancer, radiation is essential in the treatment of nasopharyngeal carcinoma (NPC). Because of the complex anatomy of the nasopharyngeal region, it is difficult to plan radiotherapy without sparing the cochlea, an important part of the auditory system, and the radiotherapy dosage to it may cause sensorineural hearing loss. In the modern era, volumetric-modulated arc therapy (VMAT) and intensity-modulated radiotherapy (IMRT) have become the gold standard in radiotherapy. With the advancement of these techniques, cochlear sparing is now possible without compromising the tumor dose.

Materials and Methods:
We reviewed 14 plans for patients with locally advanced NPC who had received radiation in our department. VMAT plans were created for patients who had IMRT radiotherapy and vice versa. Both approaches were evaluated in terms of cochlea sparing while maintaining the coverage of the planned target volume (PTV).

Results:
Our study compared the results of two different radiation techniques for locally advanced NPC, IMRT, and VMAT in 14 cases, and we found that VMAT was associated with a lower maximum dose to the cochlea, a lower mean dose to the cochlea, a higher PTV D98% (Gy), a lower PTV D2% (Gy), a higher PTV V95% (%), a lower heterogeneity index, and a higher conformity index. The P value for each comparison was <0.05, which indicates that the difference is statistically significant. These results suggest that VMAT is a better radiation technique than IMRT for locally advanced NPC. VMAT is associated with a lower dose to the cochlea and other organs at risk, which can improve the quality of life and survival of patients.

Conclusion:
These results suggest that VMAT is a better radiation technique than IMRT for locally advanced NPC. VMAT is associated with a lower dose to the cochlea and other organs at risk, which can improve the quality of life and survival of patients.
Evaluation of International Atomic Energy Agency Technical Report Series-483 Detector-specific Output Correction Factor for Various Collimator Systems
Authors: Kannan Mageshraja , Saminathan Sathiyan , Chandraraj Varatharaj , Raj D. Gowtham , Ganesh K. M. 
Published: September 2023

Aim:
In this study, a 6MV flattening filter (FF) and 6MV FF Free (FFF) photon beam small-field output factors (OF) were measured with various collimators using different detectors. The corrected OFs were compared with the treatment planning system (TPS) calculated OFs.

Materials and Methods:
OF measurements were performed with four different types of collimators: Varian Millennium multi-leaf collimator (MLC), Elekta Agility MLC, Apex micro-MLC (mMLC) and a stereotactic cone. Ten detectors (four ionization chambers and six diodes) were used to perform the OF measurements at a depth of 10 cm with a source-to-surface distance of 90 cm. The corrected OF was calculated from the measurements. The corrected OFs were compared with existing TPS-generated OFs.

Results:
The use of detector-specific output correction factor (OCF) in the PTW diode P detector reduced the OF uncertainty by <4.1% for 1 cm × 1 cm Sclin. The corrected OF was compared with TPS calculated OF; the maximum variation with the IBA CC01 chamber was 3.75%, 3.72%, 1.16%, and 0.90% for 5 mm stereotactic cone, 0.49 cm × 0.49 cm Apex mMLC, 1 cm × 1 cm Agility MLC, and 1 cm × 1 cm Millennium MLC, respectively.

Conclusion:
The technical report series-483 protocol recommends that detector-specific OCF should be used to calculate the corrected OF from the measured OF. The implementation of OCF in the TPS commissioning will reduce the small-field OF variation by <3% for any type of detector.
Effects of oral melatonin premedication on hemodynamic responses to intubation, anesthetic requirements and postoperative sedation: A randomized trial
Authors: Rajan Sunil , Abubaker Reema , Kala Revathy Ajayachandran , Sasikumar Niranjan Kumar , Kannan Mani Vignesh , Kumar Lakshmi 
Published: September 2023

Abstract

Background and Aims:
Sedative effects of melatonin may have an additive effect on general anesthesia (GA). We compared hemodynamic response to intubation following oral premedication with melatonin versus placebo. Induction dose of propofol, isoflurane and fentanyl consumption were also compared.

Material and Methods:
This prospective, double-blinded study was conducted in fifty patients randomized into two equal groups. Group M received oral melatonin 6 mg and group P a placebo two hours before surgery. All patients were induced with intravenous propofol of 1.5–2.5mg/kg till loss of response to verbal commands, three minutes after vecuronium, laryngoscopy was done and trachea was intubated. Heart rate (HR) and mean arterial pressures (MAP) were recorded before premedication, before induction, immediately after induction and then at 1,3,5 and 10 minutes after intubation.

Results:
Mean HR was comparable in both groups throughout the study period. Group M had significantly lower MAP before induction and immediately after induction (P < 0.05). At all other time points MAP remained comparable in both groups. Mean isoflurane consumption was significantly lower in group M compared to group P (14.8 ± 4.2 vs 19.7 ± 3.2 mL). Propofol requirement for induction was also significantly lower in group M (102.4 ± 19.6 vs 122.4 ± 26.3mg). Intraoperative fentanyl consumption was comparable.

Conclusion:
Oral premedication with melatonin 6mg administered two hours before surgery significantly reduced MAP before and after induction of GA with a significant reduction in dose of propofol requirement.Titrating induction dose of propofol till loss of response to verbal commands did not effectively attenuate responses to laryngoscopy and intubation following melatonin oral premedication.
Efficacy of Ayurveda formulations, Panchamrit Lauha Guggulu and Panchguna Taila in the management of Cervical Spondylosis (Greevagraha)
Authors: Negi Vineeta K. , Singh Renu , Chandla Anubha , Sharma Omraj , Sharma Surinder K. , Rana Rakesh , Singhal Richa , Sharma Bhagwan S. , Khanduri Shruti , Kumar Adarsh , Srikanth Narayanam 
Published: September 2023

BACKGROUND:
Cervical spondylosis (CS) is a degenerative disease of intervertebral discs with the formation of osteophytes in the cervical vertebrae. Based on the similarity in symptoms such as intermittent neck and shoulder pain, pain and stiffness radiating to the shoulder or occipital region, restricted neck movements, numbness, and weakness in arms, hands, and fingers, CS can be correlated with Greevagraha—a disease of the neck narrated in Ayurveda. In Ayurveda, the formulations Panchamrit Lauha Guggulu (PLG) and Panchguna Taila (PGT) have been narrated in treating musculoskeletal disorders.

OBJECTIVE:
The objective of the study was to evaluate the clinical efficacy and safety of PLG and PGT in the management of CS.

MATERIALS AND METHODS:
It was a prospective, single-arm, open-label clinical study. A total of 63 patients aged 30–60 years diagnosed with CS have been treated with PLG 250 mg twice a day orally with lukewarm water after food and an external application of PGT at the neck region. The total duration of the treatment was 12 weeks, with a follow-up every 2 weeks and a post treatment follow-up after 2 weeks of the treatment. The outcome measures include change in Northwick Park Neck Pain Questionnaire (NPNPQ) score, Visual Analog Scale (VAS) score for cardinal features, SF-36 (research and development [RAND]) Health Survey Score, and incidence of adverse events (if any).

RESULTS:
At baseline, X-ray findings of all participants (100%) had degenerative changes at different levels of cervical vertebrae; however, posttreatment X-ray findings (on the 84th day of study) showed normal X-ray findings in eight participants (5.04%). A significant reduction in the total VAS score (P < 0.001) was observed on day 84 and day 98. The mean NPNPQ score reduced significantly (P < 0.0001), and a highly significant change was observed in the SF-36 Health Survey Score (P < 0.0001). No adverse events were reported during the study period.

CONCLUSIONS:

PLG and PGT have shown promising outcomes in managing CS and were also safe. However, randomized control trials with adequate sample size, standard care as control, and a longer follow-up may be planned to establish the findings of this preliminary study.
Efficacy of Phala Ghrita and cow ghee in the management of oligozoospermia
Authors: Varsakiya Jitendra Nathabhai , Goyal Mandip , Thakar Anup , Donga Shilpa 
Published: September 2023

BACKGROUND:
Oligozoospermia is one of the major causes of infertility in males. A special branch of Ayurveda called Vajeekarana (aphrodisiac therapy) is specially related to management of infertility. Phala Ghrita (PHG) is an Ayurved formulation specially indicated in infertility due to oligospermia, and it is also narrated that cow ghee (CG) also possesses spermatogenic efficacy. Therefore, the present work was planned to compare the clinical efficacy of PHG and CG on oligozoospermia.

MATERIALS AND METHODS:
Ninety-nine male patients suffering from oligospermia and having sperm count less than 15 million/mL were selected for the study. Patients’ underwent Ayurveda Bio-cleansing procedure by using Haritakyadi Yoga–a laxative Ayurveda formulation. PHG and CG at a dose of 10 g, twice daily were administered in 51 and 42 patients, respectively, with warm milk for 8 weeks. Assessment of seminal parameters were done before and after completion of treatment.

RESULTS AND DISCUSSION:
Statistical data showed that PHG and CG showed a significant (P < 0.001) change in sperm such as increase in sperm motility, decrease in abnormal forms of sperm, and increase in semen volume. However, the effect of PHG is found to be better than CG except in increasing semen volume.

CONCLUSION:
PHG and CG both have significant spermatogenic potential when administered after bio-cleansing of body by using Haritakyadi Yoga.

Study registration:
CTRI/20164/01/006559