bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting self-regulated learning, autonomy, and self-efficacy of EFL learners through authentic assessment in EFL classrooms
Authors: Ismail Sayed M. , Nikpoo Iman , Prasad K. D. V. 
Published: May 2023

AbstractThe present research tried to prompt self-regulated learning, autonomy, and self-efficacy of EFL learners through authentic assessment in Iranian EFL classrooms. To do so, 57 Iranian EFL learners were chosen and assigned to two equal groups; the Experimental Group (CG) and the Control Group (CG). Then, three questionnaires were administered to assess the respondents’ self-regulated learning, autonomy, and self-efficacy before doing the treatment. After that, the EG received the treatment via using authentic assessments whereas the CG received the instruction through non-authentic assessments. After teaching 15 English passages to both groups, three post-tests were administered to them to determine the effects of the treatment on their self-regulated learning, autonomy, and self-efficacy. Lastly, the attitude questionnaire was given to the EG students to assess their attitudes toward implementing authentic assessments in EFL classes. The outcomes of ANCOVA revealed that there were significant differences between the post-tests of the EG and the CG. The results displayed that the EG noticeably outdid the CG in self-regulated learning, autonomy, and self-efficacy post-tests. In addition, the results showed that the EG held a positive attitude toward using authentic assessment in EFL classes. It can be concluded that applying authentic assessment in EFL classes can bring about constructive impacts for EFL learners. The findings of this investigation can be extremely valuable for EFL teachers and material designers to consider the impacts of authentic assessments and invest more in using these sorts of assessments.
Sucrose metabolism analysis in a high sucrose sugarcane mutant clone at a mature stage in contrast to low sucrose parental clone through the transcriptomic approach
Authors: Khan Qaisar , Qin Ying , Guo Dao-Jun , Chen Jiao-Yun , Zeng Xu-Peng , Mahmood Amir , Yang Li-Tao , Liang Qiang , Song Xiu-Peng , Xing Yong-Xiu , Li Yang-Rui 
Published: May 2023

Abstract
Background
The significance of sugarcane as a major crop is due to the production of sucrose and a significant source of ethanol. Sucrose phosphate synthase (SPS), Sucrose synthase (SuSy), and cell wall invertase (CWIN) genes regulate sucrose metabolism and accumulation in high sucrose sugarcane; such genes are significant for manipulating sucrose content. The current study explored the genes associated with sucrose metabolism and accumulation in a high sucrose sugarcane mutant clone GXB9 compared to its low sucrose parental clone B9.

Results
A total of 100, 262 differentially expressed genes were obtained, and 69, 637 (69.46%) got annotation in public databases COG, GO, KEGG, KOG, Pfam, eggNOG, NR, and Swiss-Prot. The SPS5 gene was significantly up-regulated in the maturing internodes of GXB9 and downregulated in B9. The SuSy and CWIN genes were up-regulated in the immature internodes; however, no expression was found in the maturing internodes of GXB9. The genes involved in trehalose synthesis were downregulated in GXB9 while up-regulated in B9. The cellulose synthase (CeS) genes were significantly up-regulated in the immature internodes than in maturing internodes of GXB9.

Conclusions
The key finding of this research suggests that the SPS5 gene has a predominant role in enhancing the sucrose accumulation ability of GXB9. No expression of SuSy and CWIN genes in maturing internodes and downregulation of trehalose genes in GXB9 may also have contributed to the higher sucrose accumulation in the stalk of high sucrose mutant. The current finding is a source for sugarcane breeding, particularly improving sucrose content.

Graphical Abstract
A systematic review and meta-analysis on the radiation dose of computed tomography in hybrid nuclear medicine imaging
Authors: Verfaillie Gwenny , Franck Caro , De Crop An , Beels Laurence , D’Asseler Yves , Bacher Klaus 
Published: May 2023

Abstract
Background
While diagnostic reference levels (DRLs) are well-established for the radiopharmaceutical part, published DRLs for the CT component of positron emission tomography/computed tomography (PET/CT) and single photon emission computed tomography/computed tomography (SPECT/CT) are limited. This systematic review and meta-analysis provides an overview of the different objectives of CT in hybrid imaging and summarizes reported CT dose values for the most common PET/CT and SPECT/CT examinations. Also, an overview of already proposed national DRLs is given.

Methods
A systematic literature search was performed to identify original articles reporting CT dose index volume (CTDIvol), dose-length product (DLP) and/or national DRLs for the most frequently performed PET/CT and/or SPECT/CT examinations. Data were grouped according to the clinical objective: diagnostic (D-CT), anatomical localisation (AL-CT) or attenuation correction (AC-CT) CT. Random-effects meta-analyses were conducted.

Results
Twenty-seven articles were identified of which twelve reported national DRLs. For brain and tumour PET/CT imaging, CTDIvol and DLP values were higher for a D-CT (brain: 26.7 mGy, 483 mGy cm; tumour: 8.8 mGy, 697 mGy cm) than for an AC/AL-CT (brain: 11.3 mGy, 216 mGy cm; tumour: 4.3 mGy, 419 mGy cm). Similar conclusions were found for bone and parathyroid SPECT/CT studies: D-CT (bone: 6.5 mGy, 339 mGy cm; parathyroid: 15.1 mGy, 347 mGy cm) results in higher doses than AL-CT (bone: 3.8 mGy, 156 mGy cm; parathyroid: 4.9 mGy, 166 mGy cm). For cardiac (AC-CT), mIBG/octreotide, thyroid and post-thyroid ablation (AC/AL-CT) SPECT/CT pooled mean CTDIvol (DLP) values were 1.8 mGy (33 mGy cm), 4.6 mGy (208 mGy cm), 3.1 mGy (105 mGy cm) and 4.6 mGy (145 mGy cm), respectively. For all examinations, high variability in nuclear medicine practice was observed.

Conclusion
The large variation in CT dose values and national DRLs highlights the need for optimisation in hybrid imaging and justifies the clinical implementation for nuclear medicine specific DRLs.
Bones and all: a new critically endangered Pantepui species of Stefania (Anura: Hemiphractidae) and a new osteological synapomorphy for the genus
Authors: Kok Philippe J. R. 
Published: May 2023

AbstractThe hemiphractid frog genusStefaniais one of the many ancient (near-) endemic lineages of vertebrates inhabiting the biodiverse Pantepui biogeographical region in the Guiana Shield Highlands of northern South America—the famous “Lost World” of Arthur Conan Doyle. Previous molecular analyses of the genusStefaniahave indicated that species boundaries and phylogenetic relationships are often incongruent with morphological traits in that clade. A substantial number of “taxonomically cryptic” species, often microendemic, remain to be described. This is notably the case for an isolated population from the summit of Wei-Assipu-tepui, a small table-top mountain at the border between Guyana and Brazil. That population was previously known asStefaniasp. 6 and belongs to theS. riveroiclade. The new species is phylogenetically distinct, but phenotypically extremely similar toS. riveroi, a taxon found only on the summit of Yuruaní-tepui in Venezuela and recovered as sister to all the other known species in theS. riveroiclade. The new taxon is described based on morphology and osteology. Data about genetic divergences within theS. riveroiclade are provided. A new synapomorphy for the genusStefaniais proposed: the presence of a distal process on the third metacarpal. Amended definitions are offered for the three other species in theS. riveroiclade (S. ayangannae,S. coxi,S. riveroi). The new species should be listed as Critically Endangered according to IUCN criteria.
Bone regeneration in inflammation with aging and cell-based immunomodulatory therapy
Authors: Kushioka Junichi , Chow Simon Kwoon-Ho , Toya Masakazu , Tsubosaka Masanori , Shen Huaishuang , Gao Qi , Li Xueping , Zhang Ning , Goodman Stuart B. 
Published: May 2023

AbstractAging of the global population increases the incidence of osteoporosis and associated fragility fractures, significantly impacting patient quality of life and healthcare costs. The acute inflammatory reaction is essential to initiate healing after injury. However, aging is associated with “inflammaging”, referring to the presence of systemic low-level chronic inflammation. Chronic inflammation impairs the initiation of bone regeneration in elderly patients. This review examines current knowledge of the bone regeneration process and potential immunomodulatory therapies to facilitate bone healing in inflammaging.Aged macrophages show increased sensitivity and responsiveness to inflammatory signals. While M1 macrophages are activated during the acute inflammatory response, proper resolution of the inflammatory phase involves repolarizing pro-inflammatory M1 macrophages to an anti-inflammatory M2 phenotype associated with tissue regeneration. In aging, persistent chronic inflammation resulting from the failure of M1 to M2 repolarization leads to increased osteoclast activation and decreased osteoblast formation, thus increasing bone resorption and decreasing bone formation during healing.Inflammaging can impair the ability of stem cells to support bone regeneration and contributes to the decline in bone mass and strength that occurs with aging. Therefore, modulating inflammaging is a promising approach for improving bone health in the aging population. Mesenchymal stem cells (MSCs) possess immunomodulatory properties that may benefit bone regeneration in inflammation. Preconditioning MSCs with pro-inflammatory cytokines affects MSCs’ secretory profile and osteogenic ability. MSCs cultured under hypoxic conditions show increased proliferation rates and secretion of growth factors. Resolution of inflammation via local delivery of anti-inflammatory cytokines is also a potential therapy for bone regeneration in inflammaging. Scaffolds containing anti-inflammatory cytokines, unaltered MSCs, and genetically modified MSCs can also have therapeutic potential. MSC exosomes can increase the migration of MSCs to the fracture site and enhance osteogenic differentiation and angiogenesis.In conclusion, inflammaging can impair the proper initiation of bone regeneration in the elderly. Modulating inflammaging is a promising approach for improving compromised bone healing in the aging population.
QPOLE: A Quick, Simple, and Cheap Alternative for POLE Sequencing in Endometrial Cancer by Multiplex Genotyping Quantitative Polymerase Chain Reaction
Authors: Van den Heerik Anne Sophie V.M. , Ter Haar Natalja T. , Vermij Lisa , Jobsen Jan J. , Brinkhuis Mariel , Roothaan Suzan M. , Leon-Castillo Alicia , Ortoft Gitte , Hogdall Estrid , Hogdall Claus , Van Wezel Tom , Lutgens Ludy C.H.W. , Haverkort Marie A.D. , Khattra Jas , McAlpine Jessica N. , Creutzberg Carien L. , Smit Vincent T.H.B.M. , Gilks C. Blake , Horeweg Nanda , Bosse Tjalling 
Published: May 2023

PURPOSE Detection of 11 pathogenic variants in the POLE gene in endometrial cancer (EC) is critically important to identify women with a good prognosis and reduce overtreatment. Currently, POLE status is determined by DNA sequencing, which can be expensive, relatively time-consuming, and unavailable in hospitals without specialized equipment and personnel. This may hamper the implementation of POLE-testing in clinical practice. To overcome this, we developed and validated a rapid, low-cost POLE hotspot test by a quantitative polymerase chain reaction (qPCR) assay, QPOLE. MATERIALS AND METHODS Primer and fluorescence-labeled 5′-nuclease probe sequences of the 11 established pathogenic POLE mutations were designed. Three assays, QPOLE-frequent for the most common mutations and QPOLE-rare-1 and QPOLE-rare-2 for the rare variants, were developed and optimized using DNA extracted from formalin-fixed paraffin-embedded tumor tissues. The simplicity of the design enables POLE status assessment within 4-6 hours after DNA isolation. An interlaboratory external validation study was performed to determine the practical feasibility of this assay. RESULTS Cutoffs for POLE wild-type, POLE-mutant, equivocal, and failed results were predefined on the basis of a subset of POLE mutants and POLE wild-types for the internal and external validation. For equivocal cases, additional DNA sequencing is recommended. Performance in 282 EC cases, of which 99 were POLE-mutated, demonstrated an overall accuracy of 98.6% (95% CI, 97.2 to 99.9), a sensitivity of 95.2% (95% CI, 90.7 to 99.8), and a specificity of 100%. After DNA sequencing of 8.8% equivocal cases, the final sensitivity and specificity were 96.0% (95% CI, 92.1 to 99.8) and 100%. External validation confirmed feasibility and accuracy. CONCLUSION QPOLE is a qPCR assay that is a quick, simple, and reliable alternative for DNA sequencing. QPOLE detects all pathogenic variants in the exonuclease domain of the POLE gene. QPOLE will make low-cost POLE-testing available for all women with EC around the globe.
Taxis of cargo-carrying microswimmers in traveling activity waves(a)
Authors: Muzzeddu Pietro Luigi , Roldán Édgar , Gambassi Andrea , Sharma Abhinav 
Published: May 2023

AbstractMany fascinating properties of biological active matter crucially depend on the capacity of constituting entities to perform directed motion,e.g., molecular motors transporting vesicles inside cells or bacteria searching for food. While much effort has been devoted to mimicking biological functions in synthetic systems, such as transporting a cargo to a targeted zone, theoretical studies have primarily focused on single active particles subject to various spatial and temporal stimuli. Here we study the behavior of a self-propelled particle carrying a passive cargo in a travelling activity wave and show that this active-passive dimer displays a rich, emergent tactic behavior. For cargoes with low mobility, the dimer always drifts in the direction of the wave propagation. For highly mobile cargoes, instead, the dimer can also drift against the traveling wave. The transition between these two tactic behaviors is controlled by the ratio between the frictions of the cargo and the microswimmer. In slow activity waves the dimer can perform anactive surfingof the wave maxima, with an average drift velocity equal to the wave speed. These analytical predictions, which we confirm by numerical simulations, might be useful for the future efficient design of bio-hybrid microswimmers.
Regulation of Alternative Splicing by Steroid Hormones
Authors: Le Billan Florian , Umogbai Gloria , Cummins Carolyn L 
Published: May 2023

Abstract
Steroid hormone signaling pathways are critical for organismal development and act through binding to nuclear receptors (NRs) driving transcriptional regulation. In this review, we summarize evidence for another—underrated—mechanism of action for steroid hormones: their ability to modulate the alternative splicing of pre–messenger RNA. Thirty years ago, pioneering studies used in vitro transfection of plasmids expressing alternative exons under the control of hormone-responsive promoters in cell lines. These studies demonstrated that steroid hormones binding to their NRs affected both gene transcription and alternative splicing outcomes. The advent of exon arrays and next-generation sequencing has allowed researchers to observe the effect of steroid hormones at the whole-transcriptome level. These studies demonstrate that steroid hormones regulate alternative splicing in a time-, gene-, and tissue-specific manner. We provide examples of the mechanisms by which steroid hormones regulate alternative splicing including 1) recruitment of dual-function proteins that behave as coregulators and splicing factors, 2) transcriptional regulation of splicing factor levels, 3) the alternative splicing of splicing factors or transcription factors that feed-forward regulate steroid hormone signaling, and 4) regulation of elongation rate. Experiments performed in vivo and in cancer cell lines highlight that steroid hormone–mediated alternative splicing occurs both in physiological and pathophysiologic states. Studying the effect of steroid hormones on alternative splicing is a fruitful avenue for research that should be exploited to discover new targets for therapeutic intervention.
Evaluation of Medical Confidentiality Breaches on Twitter Among Anesthesiology and Intensive Care Health Care Workers
Authors: Pineau Iris , Pineau Marc , Selim Jean , Compère Vincent , Besnier Emmanuel , Zoé Demailly , Popoff Benjamin , Clavier Thomas 
Published: May 2023

BACKGROUND:
With the generalization of social network use by health care workers, we observe the emergence of breaches in medical confidentiality. Our objective was to determine, among anesthesiology and intensive care health care workers, the rate of medical confidentiality breaches among professional tweets.

METHODS:
We performed a retrospective analysis of public Twitter data available through the official Twitter application program interface. The profiles of anesthesiology and intensive care professionals were identified thanks to keywords in their biography. All the tweets with a photograph and all the text-only tweets containing at least one specific keyword related to anesthesiology or intensive care were extracted. We selected only the tweets with a health care–related character. Then, we analyzed 10% of the tweets with a photograph and 10% of the text-only tweets extracted and noted those presenting a breach of medical confidentiality.

RESULTS:
After a first screening of 12,705 accounts, we manually analyzed 431 tweets with photograph(s) and 9000 text-only tweets from 1831 accounts. We found 44 (10.2%) breaches of medical confidentiality among the photographs and 76 (0.8%) among text-only tweets. These 120 problematic tweets came from 96 profiles (96/1831; 5.2%); 3.7% of North American profiles breached medical confidentiality versus 6.3% of profiles from other areas; P = .03. When comparing the distribution of the number of followers and tweets, accounts with breach of medical confidentiality tweets had more tweets and followers than profiles without (both P < .0001).

CONCLUSIONS:
We found a significant proportion of tweets with breach of medical confidentiality among anesthesiology and intensive care professionals accounts.
“A Clean Conscience behind the Dark Bars”
Authors: Altınay Rüstem Ertuğ 
Published: May 2023

AbstractThe iconification of political prisoners enhances their visibility, credibility, and power. Nevertheless, iconification may also reduce, reimagine, or otherwise distort the biographies and experiences of political prisoners. Moreover, iconicity’s blurring of prisoners’ views and activities may result in the recirculation of their stories in the service of political projects that do not fully align with their own. The incarceration of the Islamist icon Şule Yüksel Şenler (1938–2019) in 1971 presents an excellent vantage point from which to analyze these dynamics and how gender informs them in fundamental ways. The diverse media representations of Şule Yüksel Şenler demonstrate how historical tropes became entangled with critical references to the law, religion, and the discourses of freedom and democracy in the iconification of an Islamist political prisoner in Cold War Turkey. Şenler’s legacy and the recent references to her story show how the tendency of iconification to occlude or distort prisoners’ ideological investments and activities may in fact enhance their ability to integrate into new political projects. This case study of a right-wing political prisoner exposes how the histories of political incarceration, combined with the discourses of injustice and victimization, may also be used to legitimize authoritarian political regimes and new incarcerations.
MVPA and Motor Proficiency between Children with ADHD and Typical Development: Associations with Sleep Quality
Authors: LIANG XIAO , QIU HUI , TSAI CHIA-LIANG , LI CHUNXIAO , SIT CINDY H. P. 
Published: May 2023

ABSTRACT

Purpose
This study examined the associations of motor proficiency with moderate-to-vigorous physical activity (MVPA) and sleep quality in children with attention-deficit/hyperactivity disorder (ADHD) and children with typical development (TD).

Methods
This cross-sectional study included 88 medical-naive children with ADHD age 6–12 yr (mean (SD) age, 8.43 (1.38) yr; 81.8% boys) and 40 age-matched children with TD (mean (SD) age, 8.46 (1.44) yr; 60% boys). MVPA was recorded by a wGT3X-BT accelerometer for 7 consecutive days. Motor proficiency was assessed using the Test of Gross Motor Development—Third Edition. Sleep quality was assessed using a self-report questionnaire.

Results
Children with ADHD spent significantly less time in MVPA daily, displayed less proficiency in locomotor and ball skills, and reported poorer sleep quality (e.g., longer sleep latency, less sleep duration, and lower sleep efficiency) than children with TD. MVPA guideline attainment and sleep duration significantly predicted locomotor skills development; in turn, locomotor skills predicted MVPA guideline attainment. MVPA and ball skills increased by age in children with ADHD.

Conclusions
Our results highlight the importance of promoting MVPA, motor proficiency, and sleep duration in children with ADHD and TD since childhood.
Expression of MAP2, GFAP, and calcium in CA3 area of a modified organotypic hippocampal slice culture treated with kainic acid
Authors: Husna Machlusil , Handono Kusworini , Sujuti Hidayat , Aulanni'am Aulanni'am , Rukmigarsari Ettie 
Published: May 2023

Background: Neurodegeneration due to neurotoxicity is one of the phenomena in temporal lobe epilepsy. Experimentally, hippocampal excitotoxicity process can occur due to kainic acid exposure, especially in the CA3 area. Neuronal death, astrocyte reactivity and increased calcium also occur in hippocampal excitotoxicity, but few studies have investigated immediate effect after kainic acid exposure. The organotypic hippocampal slice culture (OHSC) is a useful model for studying the neurodegeneration process, but there are still many protocol differences. In this study, minor modifications were made in the OHSC protocol. Methods: OHSC was obtained from two healthy wild type Wistar rats aged P10. Healthy culture slices were obtained and lasted up to 10 days in vitro (DIV 10). Bath application of kainic acid for 48 hours in DIV 10 followed by observation of its initial effects on neurons, astrocytes, and calcium via the expression of MAP2, GFAP, and intracellular calcium, subsequently. Results: After 48 h of kainic acid administration, there was a significant increase in intracellular calcium (p = 0.006 < α), accompanied by a significant decrease in MAP2 (p = 0.003 < α ) and GFAP (p = 0.010 < α) expression. Conclusion: These findings suggest early neuronal and astrocyte damage at the initial onset of hippocampal injury. This implies that astrocyte damage occurs early before an increase in GFAP that characterizes reactive astrogliosis found in other studies. Damage to neurons and astrocytes may be associated with increased intracellular calcium. It is necessary to develop further research regarding expression of calcium, MAP2, and GFAP at a spatial time after exposure to kainic acid and strategies to reduce damage caused by excitotoxicity.