bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Derivatization on the Metabolite Profiling of the Cadmium-Tolerant Mangrove Fungus Trichoderma atroviride Using GC-MS Analysis
Authors: Jamali Siti Athirah Mohamad , Azizan Kamalrul Azlan , Baharum Syarul Nataqain , Taib Mariam 
Published: December 2022

The mangrove fungus Trichoderma atroviride was found to be tolerant to the heavy metal cadmium and it is of high interest to profile its metabolites to gain insight into its response to cadmium toxicity. This study aimed to investigate the effect of derivatization agents on the number and types of metabolites present in the cadmium-tolerant T. atroviride, detected using GC-MS analysis. The intracellular and extracellular metabolites of T. atroviride treated with cadmium for ten days were derivatized using silylation and alkylation reactions. The results showed that a higher number of metabolites were identified when the three different derivatization agents were used: BSTFA, TBDMSTFA, and MCF. More types of metabolites were identified by silylation, making it suitable for non-targeted metabolites profiling study. Silylation is efficient for the analysis of sugars and their derivatives while alkylation is suitable for a targeted study involving amino acids and organic acids. Statistical analysis for the data set of identified metabolites was performed using Metaboanalyst 3.0 followed by visualization using Partial Least Square-Discriminant Analysis. The plots showed clear separations of metabolites between the different types of derivatization agents and between control and cadmium-treated samples. A more comprehensive metabolite profile of T. atroviride obtained using different derivatization agents in this study, followed by distinct metabolites detected between control and treated samples, will provide good baseline information for future investigations including the pathways and biomarkers responsible for the fungal tolerance to cadmium toxicity.
Investigation of Smart City Components by AHP-BWM-FUCOM and DEMATEL Methods
Authors: EKİN Emre , SARUL L. Sinem 
Published: December 2022

The concept of smart city has emerged with the development of technology in the 21st century, based on the ideal city concept introduced in the 19th century.
Although over forty smart city components can be counted in the literature, the main six elements which are smart economy, smart people, smart governance, smart mobility, smart environment and smart living are mostly included in the studies.
In this study, these six main smart city components is weighted by one of the most widely used MCDM techniques AHP, DEMATEL and more recently developed techniques BWM and FUCOM. The results of comparison is given at the end.
Ocupação de espaços públicos pela Cidadania - uma alternativa possível
Authors: Virgílio Fabiana Cristina , Cortese Tatiana Tucunduva Phillippi 
Published: December 2022

Quando dirigir o olhar a um espaço em degradação permanente, muda a tônica ditada pela contemporaneidade e o quanto exercer o sentimento de pertença auxilia na legitimação da palavra CIDADANIA. A situação da Praça das Bandeiras era mais uma na história das cidades contemporâneas, quando a violência urbana se achega e utiliza os espaços públicos para trazer insegurança e desmobilizar a verdadeira vocação de uma praça, que é encontro e convivência. Para reverter esse processo de degradação, a sociedade civil organizada se reúne em uma Associação para ocupar culturalmente a praça e reavivar a vocação desse espaço tornando possível a democratização da cultura com: Sessões de Cinema, poesia/ saraus, teatro, música... transformando o espaço que antes era tomado pelo tráfico de drogas em música ao pôr do sol aos domingos, espetáculos de teatro de Rua, em Saraus de poesia, jogos culturais, árvores de livros (para a sociedade levar para a casa), Geladeiroteca, festivais de música e muito mais. Invocando o sentimento de pertencimento para a transformação de um território, pelo foco da cultura e do exercício da cidadania.
Vivência Permacultural para Idosos
Authors: Verni Bianca Batelani 
Published: December 2022

O presente trabalho busca estudar as relações do crescimento da população idosa, fenômeno que vem sendo protagonista nas discussões que impactam o futuro, além de entender como isso reflete na estruturação da Instituições de Longa Permanência para Idosos, os populares “asilos”, demonstrando como esse modelo não satisfaz as reais demandas e necessidades desse público, oferecendo o mínimo para o bem-estar dos idosos. Visto isso, objetiva-se implantar um modelo de instituição, destinada a idosos de baixa renda na cidade de Birigui, a fim de suprir essas carências, que não são somente físicas, mas também afetivas e psicológicas. Esse novo modelo de instituição se baseará em preceitos permaculturais, procurando desenvolver um ambiente comunitário, solidário e sustentável em harmonia com a natureza e demais conceitos como a sustentabilidade, agroecologia e bioconstrução.
Palavras-Chave: Idosos, Permacultura, Sustentabilidade, Bioconstrução, Instituição Pública
Perceived Organizational Support and Voice Behavioural Performance in Public Organizations in Nigeria
Authors: Akhimien Okharedia Goodheart , Kadiri Alasa Paul 
Published: December 2022

This study investigated the relationship between perceived organisational support and voice behavioural performance in selected public sector organisations in Nigeria. Two dimensions of employees’ voice behavioural performance, which are, promotive voice behavioural performance and prohibitive voice behavioural performance were empirically examined. The organisational support dimensions explored are: employers support, supervisors support, and fellow employees support. A survey research design which employed the use of the questionnaire was used to collect the needed date from the respondents. Data which was generated from three hundred and fifty selected respondents were analyzed using descriptive statistics such as percentages analysis and inferential statistics such as multiple regression. The result of the study revealed that all the three dimensions of organisational support examined, that is, employers support, supervisors support, and fellow employees support were significantly related to promotive voice behavioural performance and prohibitive voice behavioural performance. It is recommended that public sector organisations in Nigeria should provide more support to its employees; direct heads of units and departments in the various ministries and government institutions to put measures in place to improve the well-being of their subordinates and also encourage employees to engage in prosocial behaviour as this might enhance behavioural voice performance.
Multi-Stage Protection Using Pixel Selection Technique for Enhancing Steganography
Authors: Abuzanouneh Khalil Ibrahim Mohammad , Hadwan Mohammed 
Published: December 2022

Steganography and data security are extremely important for all organizations. This research introduces a novel stenographic method called multi-stage protection using the pixel selection technique (MPPST). MPPST is developed based on the features of the pixel and analysis technique to extract the pixel's characteristics and distribution of cover-image. A pixel selection technique is proposed for hiding secret messages using the feature selection method. The secret file is distributed and embedded randomly into the stego-image to make the process of the steganalysis complicated. The attackers not only need to deter which pixel values have been selected to carry the secret file, they also must rearrange the correct sequence of pixels. MPPST generates a complex key that indicates where the encrypted elements of the binary sequence of a secret file are. The analysis stage undergoes four stages, which are the calculation of the peak signal-to-noise ratio, mean squared error, histogram analysis, and relative entropy. These four stages are used to demonstrate the characteristics of the cover image. To evaluate the proposed method, MPPST is compared to the standard technique of Least Significant Bit (LSB) and other algorithms from the literature. The experimental results show that MPPST outperforms other algorithms for all instances and achieves a significant security enhancement.
Assessment of clinical, biochemical and radiological profile of SARS COVID-19 patients in a tertiary healthcare hospital and subsequent prediction of prognostic indicators
Authors: Jayaram Shreya , Rao Raghavendra 
Published: December 2022

Background: To describe the clinical characteristics and outcomes of SARS COVID-19 patients in Karnataka state, India and to evaluate risk factors and laboratory abnormalities leading to adverse outcomes. Radiological chest x-ray features of patients were studied and further elaborated upon.Methods: A hospital-based, single centre observational prospective study was conducted which included 144 adults with confirmed cases of SARI as per the accepted definition admitted to Kasturba hospital, Manipal in a one-month time frame period who tested positive for the coronavirus. Symptoms taken into consideration were cough, sore throat, breathlessness, and fatigue commonly seen co-morbidities were diabetes, hypertension, IHD and chronic kidney disease. Laboratory variables taken were haemoglobin, CRP, urea, creatinine, NT-Pro BNP and LDH. Final outcome measured was the need for mechanical ventilation which correlates to disease severity. Multivariate logistic regression analysis was done for NLR ratio. Also evaluated was the abnormal radiological appearance of affected patients on chest X-ray describing baseline status and distribution of infiltrates.Results: Median age of patients was 58 years, 69.4% males (100) and the remaining 30.6% females (44). The mean symptom duration before hospitalization was 3.5 days with a maximum of 30 days and minimum of 1 day. Breathlessness (52.8%) was most commonly seen symptom with almost half (50%) patients having pre-existing hypertension as a co-morbid condition. 46.5% patients had pre-existing diabetes. Abnormal lab values of parameters studied was shown to have a positive association with disease severity. Chi square statistics for decreased haemoglobin was 18.5363, urea-44.6823, creatinine-33.4771, NT PRO BNP-39.812, LDH- 81.3515, with p<0.00001 in all cases. An elevated NLR ratio was found in most patients requiring mechanical ventilation with a significant p<0.05. The 59.7% patients had bilateral lung involvement on CXR with 38.9% showing basal infiltration.Conclusions: Early detection of laboratory abnormalities in haemoglobin, urea, creatinine, NT pro BNP, LDH and NLR ratio were found to be efficacious in forecasting need for mechanical ventilation in COVID-19 patients and can help better predict patient outcomes to avoid delays in management and care.
Comparison of Amritsar scoring system using quantitative contrast enhanced ultrafast magnetic resonance mammography and diffusion technique with conventional Kaiser scoring system
Authors: Kapoor Atul , Kapur Aprajita , Sidhu Bholla Singh , Singh Paramjit , Brar Navjot , Singh Jasdeep 
Published: December 2022

Background: Magnetic resonance imaging (MRI) mammography has been recommended as a problem solving tool in patients with breast lump for which newer imaging protocols like abbreviated, ultrafast MRI and diffusion MRI are now available. For interpretation lexicons like BIRADS and Kaiser score system are available but there is a scope for improvement of results and need for newer lexicons.Methods: Retrospective study of 175 patients of breast lump who had MRI mammography was done. The lesions were labelled as malignant by Kaiser score system (KSS) and a newer scoring system “Amritsar score system” (AMSS). Final diagnoses was confirmed by histological examination with hormone and Her2neu receptor studies. Statistical analysis was done for correlation, sensitivity, specificity and accuracy along with area under curves and the results compared.Results: Study comprised 32/175 patients with malignant nodules. Mean age of 47.2 (range: 44.2-49.6) years with mean nodule size of 2.2 cm (range 1.8-5.5 cm). ADC and Ktrans, Kep, TTE, MS and IAUC60 showed high correlation with size of malignant nodule. Sensitivity of detection was 87.4%, 87.5%, 88.6%, 71.8% and 80% respectively for ADC, Ktrans, Kep, TTE, MS and IAUC60 while specificities were 94, 88.7, 88.7% 90% and 90% respectively. The sensitivity, specificity and accuracy for KSS and AMSS were 62.5%, 88.9%, 72% and 96.5%, 90% and 94% respectively.Conclusions: AMSS is more accurate than KSS and improves the sensitivity and specificity of cancer detection. Ktrans and ADC imaging parameters not only show high sensitivity for cancer detection but also have a good correlation with the size and nuclear grade to be used as imaging biomarkers.
Prospective study of predictive factors for conversion of laparoscopic cholecystectomy to open surgery
Authors: Shah Unnati , Sukhlecha Akhilesh 
Published: December 2022

Background: Gallstone diseases are a common gastrointestinal illness in the general population that frequently requires hospitalization with a prevalence of around 11% to 36% The goal of this study was to determine the conversion rate and identify the factors responsible for the conversion of laparoscopic cholecystectomy (LC) to open cholecystectomy (OC).This study intends to evaluate the causes of conversion from LC to OC and to establish the efficacy and safety of the procedure. Methods: This case series was conducted in the department of general surgery at Gujarat Adani institute of medical science, Bhuj, Gujarat from October 2018 to July 2020. Patients more than 18 years of age presenting with OPD with symptomatic gallstones and patients with acalculous cholecystitis were included in the study. Patients with dilated CBD (>8 mm in diameter), jaundice, gallbladder malignancy, and perforated gallbladder were excluded. Results: A total of 100 patients were included in the study; 68 were females and 32 were males. Eleven (11%) patients were converted to OC. The most common cause of conversion was dense adhesions followed by obscure anatomy at Calot's triangle. Other common causes were bleeding, bile leakage, visceral injuries, and instrument failure Conclusions: Most common cause of conversion from LC to OC was dense adhesions causing obscure anatomy at Calot's triangle followed by bleeding and CBD injury.
A prospective study on vertical mattress suture and subcuticular suture skin closure techniques in inguinal hernia repair cases
Authors: R. Saranya , S. Padma , K. Manoj S. 
Published: December 2022

Background: Sutures are used to keep tissues in place until enough healing has taken place to produce endogenous wound strength. The method of closure should be quick, simple, affordable, and successful while maximizing the cosmetic of the wound and patient outcomes. So, this study was an attempt to compare subcuticular and vertical mattress suture techniques in inguinal hernia repair. Methods: A prospective study was carried out among patients posted for inguinal hernia repair. The wound closure of the first 25 patients was done using a subcuticular suture and another by classical vertical mattress suture. Duration required for wound closure, complications, postoperative pain and cosmesis were assessed and analyzed using descriptive and inferential statistics. Results: The duration required for wound closure was higher in the subcuticular suture compared with the classical vertical mattress suture. There was no significant difference in post-operative pain, wound complications and wound cosmesis in both groups. Conclusions: Among cases with inguinal hernia repair there was no superiority in subcuticular and classical vertical mattress sutures from the patient side.
Association of Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in breast cancer patients of Bihar: Case-control study
Authors: Verma Tanu , Lal Rohit , Kumar Manish , Kumar Uday , Shahi S. K. 
Published: December 2022

Breast cancer (BC) is the most frequent cancer in women globally and the main cause of cancer-related mortality. MTHFR C677T is a functional polymorphism in the genes encoding a one-carbon metabolism enzyme that affect folate metabolism and may be associated with breast cancer susceptibility. We have conducted a case-control study taking 100 cases of diagnosed breast cancer and 100 healthy control. PCR-RFLP technique was used to detect the genotype of C677T polymorphism in the study population.About 35% of cases had normal genotype(C677C), 49% had the genotype C677T & rest 16% had the genotype T677T for MTHFR. Among control the frequency of C677C genotype was 55% C677T was 40% & T677T was 5%. C677T & T677T MTHFR genotype were more common in case as compared to control [χ2 (2, N = 200) = 11.12, p = 0.004]. T allele frequency was significantly higher in case (40%) as compared to control (5%) [χ2 (1, N = 400) = 10.91, p = 0.001]. Cases with MTHFR 677TT genotype had a significantly higher risk of BC than 677CC individual [OR=1.64 (1.03 to 2.10)] and increasing T-allele was significant in BC [OR=1.07 (0.71 to 1.33)] (P for trend = 0.04).C677T of MTHFR gene has a strong correlation with breast cancer in the studied population and can be used as a prognostic marker for the development of breast cancer.
Evaluation of Dyslipidemia with HbA1c levels in Diabetes Mellitus
Authors: Malathi R. D. , Fatima Aliya , Ahmed Mohd Iqbal 
Published: December 2022

Diabetes is a metabolic disease which has hyperglycaemia resulting from defects in insulin secretion or action. HbA1C is one of the most reliable test used for estimation of glycaemic index. Diabetes is often accompanied by undiagnosed dyslipidaemia, characterized by increased triglyceride level, decreased high-density lipoprotein levels and increase in low-density lipoprotein (LDL) particles. Diabetes is a significant risk factor for atherosclerotic cardiovascular disease (ASCVD). The purpose of this study was to correlate lipid parameters with different levels of HbA1c to assess dyslipidaemia with diabetic status. An observational study was conducted in biochemistry laboratory at Gandhi Hospital, Telangana.After informed consent, 5ml of venous blood sample was collected in plain tube and EDTA tube from diabetic and apparently non diabetic patients. Estimation of serum triglycerides was done by glycerol phosphate oxidase peroxidase, serum cholesterol by cholesterol oxidase method, serum HDL by direct clearance method in Beckmann Coulter AU 500 and HbA1c was estimated by high performance liquid chromatography in Bio-Rad D10.A total of 90 samples were divided into 3 groups according to their HbA1c results. Group 1 with HbA1c less than 5.6, Group 2 with HbA1c 5.6-7 and Group 3 with HbA1c more than 7.The present study showed no variation in the lipid profile in Group1 with HbA1c &#60; 5.6. In Group 2 with HbA1c between 5.6-7, there was significant increase in LDL cholesterol. In Group 3 with HbA1c &#62;7 there was marked increase in LDL, serum triglyceride. Hence we can conclude that dyslipidemia is increased in poor glycemic control and evaluation of HbA1c may also be used as a predictor of dyslipidaemia in diabetics.
Angiotensin II type I receptor gene polymorphism in essential hypertensive and normotensive subjects
Authors: Sharma Hariom , Patel Sapna , Sharma Shreyansh , Rao Drashti , Dholakia Jagruti , Baraiya Hina 
Published: December 2022

Hypertension is a polygenic disorder that involves a complex interplay of genetic and environmental factors. Genes that influence the renin–angiotensin–aldosterone system (RAAS) appear to be particularly important among the many potential candidate components of EHT. The RAAS system plays an important role in BP regulation by maintaining vascular tone and renal hemodynamics. Most of these are mediated via the activation of the angiotensin II type 1 receptor (AT1R). Untreated hypertension increases the risk of developing cardiovascular diseases such as stroke, myocardial infarction, ischemic heart disease, and, target organ damage. A polymorphism in the 3’ untranslated region of the AT1R gene has been described with either an adenine (A) or a cytosine (C) base (A/C transversion) at the 1166 position that is associated with increased blood pressure. A total of 170 hypertensive patients and 170 normal subjects were included in the present study. Genomic DNA was isolated from peripheral blood leukocytes, amplified with PCR, and separated with gel electrophoresis to determine the AT1R genotype. On electrophoresis, the genotypes A/A-359 bp, A/C-220+139 bp, and C/C-220, 139, and 359 bp were identified. In hypertensive patients, the CC genotype was present in 21.18 percent (n = 36) as compared to 11.76 percent (n = 20) in non-hypertensive patients. In this study AT1R CC homozygote was significantly associated with hypertension (p = 0.019). The odds ratio related to the association of the prevalence of the CC genotype with hypertension was 2.014 (95% CI: 1.112–3.650).: From the present study, it is concluded that AT1R gene polymorphism is associated with hypertension, and with the CC genotype, the risk of hypertension increases in comparison to AC and AA. A allele is not significantly associated with hypertension, but the recessive and co-dominant modes of inheritance of allele C (CC) and its co-dominant mode (AC) are a genetic predisposition to hypertension.
Assessment of Lab request forms: How do our clinicians communicate with Laboratory?
Authors: Trivedi Dhiraj J 
Published: December 2022

Central clinical laboratory is an important division in the health care setup. Laboratory results help clinical decisions, follow up care and ensure patient safety. Laboratory request Form [LRF] is an important medium between the patient, a treating clinician and Laboratory. A meticulously filled LRF is important for patient care. Providing accurate and complete information in LRF is the doctor's responsibility. Erroneous LRF will have an impact on the quality of laboratory results. Present study evaluates the degree of completeness and correctness of quality indicators on laboratory request forms [LRF] to examine preanalytical standards of laboratory services.This study is a single center, prospective, cross sectional, descriptive type conducted at a 650 bed teaching hospital from Gujarat. In the span of a six months study period, 3735 [20% of total] LRFs were selected by simple random sampling method from the total LRF received at OPD blood collection center. They were analyzed for patient, clinician and sample identifier quality indicators along with completeness and correctness. Qualitative information was converted to quantitative by using two point scale, 0 score for incomplete information and 1 score for complete information.Among patient identifier quality indicators name, age, gender and location were filled in more than 75% forms whereas, very poorly filled 2% provisional diagnosis and 42% MRD number. Clinician identifier quality indicator was attended to in less than 50% forms. Time and date of request were absent on 100% forms. Sample identifier quality indicator shows 97% forms with the nature of the sample and 92% having investigation requests. Test requests on one third forms were invalid and inappropriate. 38% forms were incomplete and inappropriate whereas 46% forms had error in filling one or other data indicators.Appropriately filled LRF communicates well with the Central clinical laboratory. It will help in providing quality reports in time and benefit clinicians to manage quality care for patients. Hand written, poorly legible, inappropriately abbreviated, erroneous LRF are misleading and may compromise laboratory service and patient safety. Training and change in attitude towards LRF writing is required to maintain the standard of the health care system.