bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Prospective Study of Dose Comparison Between Deep Inspiratory Breath Hold (DIBH) Technique Versus Free Breathing (FB) Technique in Reducing Cardiac Dose in Left Sided Post Mastectomy Breast Cancer Patients Treated With Radiotherapy
Authors: Ravishankar Dev , Sunil Devika , Yadala Nalini , Gandhi Arun , Mohanty Kirti Ranjan 
Published: December 2022

Abstract
Cardiac mortality and morbidity during breast cancer treatment can be caused by both radiotherapy and chemotherapy. As chemotherapy cannot be omitted due its proven overall survival benefit, newer radiotherapy techniques like DIBH with real time position monitoring (RPM), have been devised to minimise dose to the heart without compromising target coverage. Very sparse prospective data look into the benefit of DIBH in chest wall radiotherapy.
AIM - To determine dosimetrically whether DIBH reduces the cardiac (heart and left anterior descending [LAD] artery) and ipsilateral lung doses compared to FB, in patients receiving radiotherapy to left side chestwall with 3D-CRT technique and to quantify it.
MATERIAL AND METHODS − 25 consecutive left sided postmastectomy breast cancer patients with minimum breath hold duration more than 15 seconds were selected. 2 plans were made for each patient using 3D-CRT technique with a dose of 40Gy in 15 fractions- first in FB (control) and the second in DIBH with RPM system. Dose volume histograms (DVH) for each patient were generated, recorded and analysed.
RESULTS - The target coverage parameters (PTV) were found to be comparable in both plans. DIBH significantly reduced (p < 0.001) the mean heart dose by 28.19% (from 5.18Gy to 3.72Gy), mean LAD dose from 25.94Gy to 16.99Gy (ie 34.50%), maximum heart distance (MHD) from 2.17cm to 1.22cm (ie by 43.54%) and all heart parameters analysed. DIBH also reduced left lung V20Gy and mean lung dose and combined mean lung doses.
CONCLUSION - With appropriate patient selection and adequate training, DIBH is an acceptable technique in reducing both cardiac and lung doses in left sided post mastectomy patients receiving radiotherapy without compromising target coverage. This could result in fewer radiotherapy-related complications even when using hypofractionated radiation doses. However, long term follow up and studies with larger sample size are warranted in the future to consolidate the significance obtained in our study.
Characterization of functional amyloid curli in biofilm formation of an environmental isolate Enterobacter cloacae SBP-8
Authors: Misra Tripti , Tare Meghana , Jha Prabhat Nath 
Published: December 2022

Abstract
The biofilm formation by bacteria is a complex process that is strongly mediated by various genetic and environmental factors. Biofilms contribute to disease infestation, especially in chronic infections. It is, therefore important to understand the factors affecting biofilm formation. This study reports the role of a functional amyloid curli in biofilm formation at various abiotic surfaces, including medical devices, by an environmental isolate of Enterobacter cloacae (SBP-8). A knockout mutant of csgA, a structural gene of curli, was created to study the effect of curli on biofilm formation by E. cloacae SBP-8. Our findings confirm curli production at 25°C and 37°C in the wild-type strain. We further investigated the role of curli in the attachment of E. cloacae SBP-8 to glass, enteral feeding tube, and foley latex catheter. Contrary to the previous studies reporting the curli production below 30°C in most biofilm-forming bacterial species, we observed its production in E. cloacae SBP-8 at 37°C. The formation of more intense biofilm in wild-type strain on various surfaces compared to curli-deficient strain (ΔcsgA) at both 25°C and 37°C suggested a prominent role of curli in biofilm formation. Further, electron and confocal microscopy studies demonstrated the formation of diffused monolayers of microbial cells on the abiotic surfaces by ΔcsgA strain as compared to the thick biofilm by respective wild-type strain, indicating the involvement of curli in biofilm formation by E. cloacae SBP-8. Overall, our findings provide insight into biofilm formation mediated by curli in E. cloacae SBP-8. Further, we show that it can be expressed at a physiological temperature on all surfaces, thereby indicating the potential role of curli in the pathogenesis.
Breast cancer: how hippotherapy bridges the gap between healing and recovery. A randomized controlled clinical trial.
Authors: Viruega Hélène , Galy Corinne , Loriette Célia , Jacquot Stéphane , Houpeau Jean Louis , Gaviria Manuel 
Published: December 2022

Abstract
Background: Breast cancer is the most diagnosed women’s cancer with a high survival rate nowadays. Because cancer is a systemic disease characterized by variable course, heterogeneity and unequal environmental inputs, disparities in the persons’ future are the norm. Despite enormous progress in early accurate detection and treatment’s effectiveness/precision, life reconstruction is well beyond the current care path. It requires comprehensive cross-sectoral approaches between different knowledge areas and deeper consideration of the challenges the patients have to deal with. The psychological and social sciences must be integrated to the physiological sciences to build a robust patient-centered healthcare practice.
Methods: We conducted a randomized open, prospective, two-arms controlled trial on the effectiveness of hippotherapy versus conventional supportive care on adult women with a diagnosis of breast cancer, after the period of primary treatment (surgery, chemotherapy, radiotherapy), according to strict inclusion and exclusion criteria. The 6-months program included, in the treated group, an initial 1-week daily hippotherapy session followed by three short 2-days sessions with an interval of 2 months between each where the patients received conventional supportive care. On the other hand, the control group received a 6-month conventional supportive care. The primary end point was quality of life, whereas cognitive performances, fatigue, anxiety and depression, and body image were the secondary end points. Measurements were done through self-reported questionnaires.
Results: We observed statistical differences for each measurement test and items between the treated and control group. Indeed, the treated group showed a significant and continuous improvement of quality of life, cognitive performance, fatigue, anxiety and depression, and body image as early as the first week after hippotherapy treatment compared to the conventional supportive care group.
Conclusions: We demonstrate through this clinical trial, the therapeutic relevance of hippotherapy, an one-health approach, as a key initial stage after cancer diagnosis and treatment to foster recovery. Furthermore, hippotherapy has a strong impact on treatments’ efficiency and reconstruction of the individuals’ shattered life and their ecosystem. This work reveals a layer of complexity that needs to be broadly considered.
Trial registration: ClincalTrials.gov NCT04350398. Registered 17 April 2020, retrospectively registered; French Clinical Trials in Cancer Register RECF3818. Registered 18 March 2019, retrospectively registered.
A Social Dynamic Approach on Floristic Composition and Physiochemical Characteristics of Karankadu Mangrove Forest, Ramanathapuram District, Tamil Nadu
Authors: V Sankara Vel , A Arunprasath , Sudhir Sreeram 
Published: December 2022

Abstract
This study investigates the diversity of angiosperms and the assessment of physiochemical studies in accordance with seasonal variations on Karankadu Mangrove Forest, Ramanathapuram, Tamil Nadu. In the study area, 62 species of angiosperms including five true mangroves Avicennia marina, Ceriops tagal, Rhizophora apiculata, Rhizophora mucronata and Bruguiera cylindrica are identified. The seasonal variations affect both the physiological status of the true mangroves present in the study area, particularly during the North East monsoon. The water and soil samples are collected for four different seasons pre-monsoon, Monsoon, Post-monsoon and summer. Abiotic components include mainly water and soil which support the mangroves. A physical parameter is mainly the temperature, pH, electrical conductivity (EC), salinity, moisture content. Chemical parameters such as dissolved oxygen (DO), biological oxygen demand (BOD), organic carbon and mineral composition – Nitrogen, Sodium and Potassium. The maximum value for most of the parameters was recorded during the post-monsoon season; it is due to a higher concentration of dissolved salts in water. During monsoon, the water is almost pure with a lesser number of dissolved salts hence most of the parameters found low values in monsoon season. The results indicate that dissolved oxygen, biological oxygen demand and Nitrogen phosphorous potassium supplements are huge during monsoon season and the lowest values are recorded in the post-monsoon season. In conclusion, it may be noted this study provides a pathway for proper utilization, conservation and acts as supplement information for Mangrove growth which may be implemented further for the Conservation and Management of mangroves.
Physics and chemical properties of the volcanic ash material adsorbed with the hexavalent chromium by x-ray absorption spectroscopy
Authors: Lontin Gristianho Lontin , Sendja Bridinette Thiodjio , Kamgne Duclair Tchana , Aquilanti Giuliana , Ben-Bolie Germain Hubert 
Published: December 2022

Abstract

X-ray absorption spectroscopy (XAS) was used to characterize the volcanic ash (VA) materials as a low-cost and natural environmental adsorbent for the sorption of Cr(VI). The batch adsorption method was employed to synthesize the sample of chromium(VI) sorbed by VA. The results showed that the Cr-VA material is in the chromium oxidation state(III) and can be described as a composition of Cr2O3 (47.9%), FeCr2O7 (45.2%), and Na2Cr2O7 (6.9%). The promotion of the 1s Cr core electron implies the electronic transitions 1𝑠→3𝑑(𝑡2𝑔) and 1𝑠→3𝑑(𝑒𝑔). The first and second shells in the Cr-VA material were coordinated to the oxygen and chromium atoms in a distorted geometrical arrangement. The adsorption mechanism was associated with the chemical reduction of Cr(VI) to Cr(III) via an ion-exchange process. The XAS technique significantly contributes to the characterization of environmental VA, which can be proposed as an economical and alternative adsorbent for the sorption of Cr from contaminated wastewater.
An intelligent diagnosis and treatment system for in-hospital cardiac arrest based on deep reinforcement learning in the Utstein style
Authors: Shao Yan , Yang Zhou , Chen Wei , Zhang Yingqi 
Published: December 2022

Abstract
Background: Both in-hospital and out-of-hospital cardiac arrest have several causes and complexities. Therefore, it is difficult to analyze and create targeted treatment plans for cardiac arrest. Moreover, even basic patient informationis insufficient or missing in many cases. To address these challenges, we developed an intelligent diagnosis and treatment system for cardiopulmonary resuscitation and restoration of spontaneous circulation to reasonably complete the diagnosis and treatment process and improve the success rate of treatment during in-hospital cardiac arrest.
Methods: Using 10 years of data from Utstein style in-hospital cardiac arrest registries in seven hospitals in China’s Hebei Province, we developed a two-stage intelligent diagnosis and treatment system based on the basic situation of different cases during cardiac arrest using deep reinforcement learning. This approach is aimed at maximizing the success rates of cardiopulmonary resuscitation and maintaining blood pressure at an optimal value during the restoration of spontaneous circulation. We employed Monte Carlo simulation to analyze the uncertainty.
Results: When comparing the actual diagnosis and treatment with those based on other models during cardiopulmonary resuscitation and restoration of spontaneous circulation in different conditions (sufficient and insufficient basic patient information), the average reward scores of the treatment plan provided by the intelligent diagnosis and treatment system developed in this study increased to 9.9–23 from 2.39.
Conclusions: The intelligent diagnosis and treatment system for in-hospital cardiac arrest based on deep reinforcement learning under the Utstein mode developed in this study can propose a reasonable diagnosis and treatment plan when in-hospital cardiac arrest occurs. Moreover, it can effectively induce cardiopulmonary resuscitation and restoration of spontaneous circulation processes in different patients even when basic patient informationis insufficient or missing.
Does repeated exposure to hydrogen peroxide induce Candida auris resistance?
Authors: Cobrado Luis , Ricardo Elisabete , Ramalho Patricia , Fernandes Angela Rita , Rodrigues Acacio Goncalves 
Published: December 2022

Abstract
Background
In order to minimize environmental colonization by microorganisms that may persist and thrive in healthcare settings, thus reducing healthcare-associated infections (HAIs), new insights over already known biocides are certainly of relevance. Although the efficacy of hydrogen peroxide (H2O2) against the emergent yeast Candida auris is moderately documented, concerns over the potential induction of resistance after repeated exposure do persist. The main objective of the present study was to evaluate the hypothetical induction of Candida auris resistance following 30 days of consecutive exposure to lethal and sublethal concentrations of H2O2. Furthermore, the authors aimed to elucidate about the rank of efficacy of H2O2 against C. auris comparing to other Candida species and whether different strains of C. auris may display different susceptibility to H2O2.
Methods
During the induction of resistance assays, both type strains and clinical isolates of Candida auris, Candida albicans and Candida parapsilosis were exposed repeatedly to defined concentrations of H2O2, for 30 days.
Results
After that period, no significant differences were found when comparing the minimal inhibitory concentration values of H2O2 in case of the induced strains versus each respective control. Moreover, H2O2 displayed similar effectiveness against all the tested Candida species and no differences were demonstrated among the distinct strains of C. auris.
Conclusions
The adoption of H2O2 solutions in routine protocols in order to promote disinfection standards against Candida auris, improving patient safety and reducing healthcare costs, is certainly welcomed.
Role of maturity status on the quality and volatile properties of mango fruits dried by infrared radiation
Authors: Li Dantong , Dai Taotao , Chen Mingshun , Liang Ruihong , Liu Wei , Liu Chengmei , Sun Jian , Chen Jun , Deng Lizhen 
Published: December 2022

Abstract
Drying characteristics and quality attributes are greatly dependent on the maturity status of fresh fruits. Hence, this work investigated the changes of quality and volatile properties of mangoes at different ripening stages (increased from RS-1 to RS-4) during infrared drying. Results showed that, the increases of hardness and total soluble solids/titratable acid during drying were varied with the ripening stage of fruits. Fruits at RS-3 obtained the highest increase in total phenolic (8.72 mg/g), the most dramatic decrease in vitamin C (5.58 mg/g), and the lowest decrease in β-carotenoid contents (56.49 mg/100g) during drying. After drying, the lower L* values, higher a* and b* values were obtained at products with higher ripeness, and the RS-2 and RS-3 achieved better color. Multivariate data analysis revealed that the aroma of dried mangoes had a clear distinction at different ripening stage, and the fruity aroma from RS-3 was the most intense. According to the comprehensive quality evaluation model, the qualities of dried mangoes were in the order: RS-3 > RS-4 > RS-2 > RS-1.
Longitudinal relationships among loneliness, social anxiety, self-esteem and self-concept in rural left-behind children: a cross-lagged study
Authors: Li Xiaolin , Zhou Zhenqian , Peng Dianhui , Lu Chunxia , Long jiayi , Xiong Yan , Dong Weixin 
Published: December 2022

Abstract
Background: Loneliness is one of the most common mental health problems among Rural Left-behind Children (RLBC) in China. Social anxiety, self-esteem and self-concept are primary correlates that are linked with loneliness, yet there is limited evidence of causal or temporal associations between these variables in RLBC. Therefore, this study explores the gender differences in RLBCs loneliness, social anxiety, self-esteem and self-concept, and aims to clarify the longitudinal cross-lagged relationship among the four.
Method: Using Children’s Loneliness Scale (CLS), Social Anxiety Scale for Children (SASC), Rosenberg’s Self-Esteem Scale (SES) and Piers-Harris Child Self-concept Scale (PHCSS) to assess the 812 RLBC. All assessments of CLS, SASC, SES, and PHCSS were measured in identical conditions at schools at the baseline (T1) and the end of the 1 year (T2). Descriptive statistics and Mann-Whitney U test were used for gender differences in variables. Partial correlation analysis of controlling gender was used to investigate the internal relationship of variables. A cross-lagged analysis were used to examine the longitudinal relationship between these variables.
Result: The result show that gender differences between CLS and SES were not statistically significant (P>0.05), but the gender difference between SASC and PHCSS was statistically significant (P<0.05). Additionally, both simultaneous and sequential correlations between CLS, SASC, SES and PHCSS were significant (all P<0.001). Lastly, CLS (β=0.491, P<0.001), SASC (β=0.229, P<0.01), SES (β=-0.140, P<0.05) and PHCSS (β=0.121, P<0.001) at T1 significantly predicted CLS at T2.
Conclusion: There were gender differences in social anxiety and self-concept of RLBC. Meanwhile, social anxiety, self-esteem and self-concept have unidirectional forecasting effect on loneliness.
Molecular pathological characteristics and tumour immune microenvironment of lung malignancies with RET rearrangement
Authors: Gao Qing-Yun , Xiao Fa-Man , Lin Xiao-cheng , Chen Yu-Qing , Li Yu-Fa , Lu Chang , Su Jun-Wei , Tan Quan-Quan , Zhang Chan-Yuan , Yang Jiao , Wu Yi-Long , Chen Hua-Jun , Yang Jin-Ji 
Published: December 2022

Abstract
Purpose
For patients with lung malignancies with RET rearrangement, the efficacy of immune checkpoint inhibitors is limited. The characteristics of the tumour immune microenvironment (TIME) and molecular pathological features of these patients have not been well elucidated.
Methods
The pathology and TIME characteristics of 29 patients with lung malignancies with RET rearrangement were retrospectively analysed, and their relationships with clinical efficacy and prognosis were investigated. Gene detection relied on high-throughput sequencing, and TIME detection was based on multiplex immunohistochemistry technology.
Results
Of the 29 patients, 86% (25/29) had adenocarcinoma, and the acinar type accounted for the greatest percentage of patients, followed by the solid type, regardless of whether the disease was early or locally advanced and metastatic. In addition, we report a novel KIF5B-RET(k24:R8) rearrangement in pulmonary sarcoma. The density of CD8 + T cells in the tumour stroma in the early-stage patients was significantly higher than that in the locally advanced and metastatic patients (P = 0.014). The proportion of M2 macrophages in the tumour stroma was significantly higher than that in the tumour parenchyma (P = 0.046). Although the difference was not statistically significant (P = 0.098), patients positive for M2 macrophage infiltration into the tumour parenchyma (≥ 5%) may have had a better prognosis.
Conclusions
A novel KIF5B-RET rearrangement variant in pulmonary sarcoma shows similar TIME characteristics to lung cancer. Among patients with lung malignancies with RET rearrangement, patients with M2 macrophage infiltration into the tumour parenchyma may have a better prognosis, but further studies with larger cohorts are needed.
More accurate detection and attribution by use of cointegrating vector time series models
Authors: Stephenson David Brian , Turasie Alemtsehai , Cummins Donald 
Published: December 2022

Abstract
Scaling factors in detection and attribution studies are typically estimated by performing a least squares regression of the observed trending variable, e.g. annual Global Mean Surface Temperature (GMST), on the equivalent variable simulated by a climate model.
This study proposes instead to obtain the scaling factors by dynamically modelling the time series as a cointegrating Vector Auto-Regressive (VAR) time series process. It is shown that a 2nd order cointegrating VAR(2) model is theoretically justified if the observed and simulated variables can be represented as a one-box AR(1) response to a common integrated forcing. The VAR(2) model can be expressed as a Vector Error-Correction Model (VECM) and then fitted to the data to obtain the cointegration relationship, the stationary linear combination of the two variables, from which the scaling factor is then easily obtained.
Estimates of the scaling factor from the VAR(2) model are critically compared to those from Ordinary Least Squares (OLS) and Total Least Squares (TLS) for GMST data simulated by a simple stochastic model of the carbon-climate system and for historical simulations from 16 climate models in the Coupled Model Intercomparison Project 5 (CMIP5) experiment. Results from the toy model simulations show that the slope estimates from OLS are negatively biased, TLS estimates are less biased but have high variance, and the VAR(2) estimates are unbiased and have lower variance and provide the most accurate estimates with smallest mean squared error. Similar behaviour is noted in the CMIP5 data. Hypothesis tests on the VAR(2) fits found strong evidence of a cointegrating relationship with the observations for all the CMIP5 simulations.
Tissue stretching is a confounding factor for the evaluation of neurodegeneration in the ever-growing killifish
Authors: Bergmans Steven , Serneels Pieter-Jan , Masin Luca , Moons Lieve 
Published: December 2022

Abstract
The fast-ageing killifish, Nothobranchius furzeri, has gained increasing attention as a promising gerontology model to study age-related processes and neurodegeneration. Interestingly, it is the first vertebrate model organism that shows physiological neurodegeneration at old age in its central nervous system (CNS), including its brain and retina. However, the fact that the killifish brain and retina are ever-growing tissues complicates studying neurodegenerative events. Indeed, recent studies showed that the method of tissue sampling, either using sections or whole-organs, has a large effect on the observed cell densities in the fast-growing CNS. In this study, we elaborated on how these two sampling methods alter neuronal frequency within the retina and how this tissue grows throughout life.
Analysis of cellular density across the different retinal layers in cryosections revealed age-dependent cell loss, that was not observed in retinal whole-mounts, as a result of an extremely fast retinal expansion with age. Using BrdU pulse-chase experiments, we were able to show that the young adult killifish retina mainly grows by cell addition, largely facilitated by the ciliary marginal zone. However, with increasing age, the neurogenic potency of this zone declines while the tissue keeps on growing. Further histological analyses revealed tissue stretching, including cell size increase, as the main driver of retinal growth in older fish. Indeed, both cell size and distance between retinal neurons augments with ageing, thereby decreasing neuronal density.
All in all, these findings highlight the need for tissue-wide counting methods to reliably quantify cell numbers in the fast-growing killifish.