bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fusobacterium detected in Barrett’s esophagus and esophageal adenocarcinoma tissues
Authors: Tahara Tomomitsu , Shijimaya Takuya , Yamazaki Jumpei , Kobayashi Sanshiro , Horitani Anna , Matsumoto Yasushi , Nakamura Naohiro , Okazaki Takashi , Takahashi Yu , Tomiyama Takashi , Honzawa Yusuke , Fukata Norimasa , Fukui Toshiro , Naganuma Makoto 
Published: June 2023

Abstract

Possible correlation of microbiota with Barrett’s esophagus (BE) and esophageal adenocarcinoma (EAC) remain unclear. Previous studies have linked Fusobacterium species to colon and esophageal squamous cell carcinomas. We examined Fusobacterium species in BE tissues of patients without and those with EAC as well as EAC tissues and also investigated its association with molecular changes, such as TP53 mutation status, telomere length and DNA methylation status. Using three different groups of biopsies obtained from non-neoplastic BE from patients without cancer (n=67; N group), with EAC (n=27; ADJ group) and EAC (n=22; T group), Fusobacterium nucreatum (F. nucleatum) and whole Fusobacterium species (Pan-fusobacterium) were examined by quantitative real-time PCR. Data were correlated with clinical and molecular characteristics. F. nucleatum was detectable in 31.8% (7/22) of T group, but not in ADJ and N groups. The amount of Pan-fusobacterium was significantly higher in T group compared to that in both ADJ and N groups (P=0.002, 0.017, respectively), while it was not significantly different among ADJ and N groups. Higher amount of Pan-fusobacterium in EAC tissues seemed to be closely associated with higher T stage (P=0.001), lymphatic invasion positive (P=0.02) and lymph node metastasis positive cases (P=0.007) and TP53 mutation negative cases (P=0.004). Higher amount of Pan-fusobacterium in non-neoplastic BE tissues (ADJ and N groups) was correlated with presence of hital hernia (P=0.04) and telomere shortening (R=-0.22, P=0.024). Our result suggested the pathogenic role of Fusobacterium species in the EAC and BE, featuring clinicopatological and molecular changes.
Trust-Based Mechanism for the Isolation of Sink Hole Attack in Wireless Sensor Networks
Authors: Sharma Swedika , Singh Arvind Kumar , Bharti Vishal 
Published: June 2023

Abstract
This paper presents a two-stage technique for detecting malicious nodes in wireless sensor networks (WSNs) where a centralized controller is absent. WSNs are dynamic networks that allow nodes to join or leave, making them vulnerable to sinkhole attacks. In this type of attack, an attacker node pretends to be a sink and intercepts data packets. The proposed technique first determines the presence of the attacker node in the network and then identifies the malicious node. The effectiveness of the technique is evaluated using NS2 simulation, considering metrics such as throughput, packet loss, and delay. The results demonstrate that the developed methodology outperforms traditional methods in detecting malicious nodes.
Copper IUD does not increase incidence of vaginal and pelvic infection; internal vaginal douching does: a cross-sectional study
Authors: Mohamed Ahmed , Youssef Ahmed Alaa , Shaaban Omar Mamdouh , Kamal Mariam , Shaltout Asmaa , Abbas Ahmed Mohamed 
Published: June 2023

Abstract
Background: We compared the rate, type, and severity of vulvovaginitis in women who are practicing internal vaginal douching (IVD) or not while using an intrauterine contraceptive device (IUCD).
A cross-sectional study on 604 consecutive attendees of the outpatient clinics using IUCD for at least 6 months prior to recruitment. Eligible participants were divided into two groups. Group I (302 women) performing IVD and group II (302 women) not practicing this habit (NIVD). The frequency of vaginal infections in IUCD users who perform IVD compared to women who were abstaining from this habit was our primary outcome.
Results: IUCD users who were practicing IVD more frequent history of vaginal infection (VI) compared with NIVD-IUCD users {260 (88.1%) versus 151(43.4%); respectively} (P<0.001). Current diagnosis of VI was significantly higher in IVD group (275; 91.05%) compared to NIVD group (115; 38.1%) (P<0.001). Bacterial vaginosis was the most common infection (287; 47.5%) followed by candida vulvovaginitis (278; 46.03%). Moreover, both types of infections were significantly more common in IVD.
Conclusion: IUCD does not increase the rate of VI; but IVD does either with or without IUCD. When VI encountered with IUCD, bacterial vaginosis is the most common type followed by candida infection.
Electrochemical and Microbial Decolourization of Congo Red Dye-contaminated Wastewater: Experimental and Computational Studies
Authors: Anene Chikwado Emmanuel , Oguzie Kanayo L. , Ogbulie Toochukwu E. , Akalezi Chris O. , Oguzie Emeka E. 
Published: June 2023

Abstract
The decolourization of congo red (CR) dye-contaminated wastewater was carried out by indirect electrochemical oxidation (IEO) and microbial degradation processes. The effect of various electrochemical parameters such as anode materials, current densities, supporting electrolytes, electrolyte concentrations and pH on the degradation of CR dye were investigated. Microbial degradation was accomplished using indigenous isolates, including Bacillus megaterium, Lactobacillus delbrueckii, Bacillus sphaericus, Pseudomonas sp., Bacillus lentus, Erwinia sp., Bacillus pumilus, Aspergillus flavus and Aspergillus niger, under varied conditions of mineral salt and time. The results of the IEO experiment show the copper anode to be more effective than the graphite anode in degrading CR dye. At the same time, the degradation rate of the dye increased with current density, electrolytes concentration and pH. The microbial degradation of CR dye increased with time, and dye to mineral salt ratio – 2:1 enhanced the degradation rate. Density functional theory (DFT) computation was deployed to investigate the degradation mechanism of CR dye which revealed the sulfonate moeity, azo chromophore and amide group to be the most susceptible sites for attack by oxidizing species.
Comparison of Mechanical Isolation Procedures of Stromal Vascular Fraction through Five Different Commercial Device
Authors: Koçak Polen , Canikyan Serli , Denizci Yağız , Beksaç Burak , Kul Yaren , Tiryaki Tunç 
Published: June 2023

Abstract
Regenerative medicine has gained immense popularity in recent years due to its potential in treating various degenerative and inflammatory conditions, including osteoarthritis (OA). Adipose-derived mesenchymal stem cells (MSCs) and stromal vascular fraction (SVF) have been identified as promising cell sources in the field of regenerative medicine and orthopedics. Adipose tissue is an abundant and easily accessible source of MSCs, making it an attractive alternative to other sources, such as bone marrow. SVF is a heterogeneous population of cells that includes MSCs, endothelial cells, pericytes, and immune cells, among others. SVF can be obtained through enzymatic digestion of adipose tissue or by mechanical means, such as centrifugation or filtration. The advantage of mechanical methods is that they have fewer regulatory restrictions and are cost-effective for clinical use. Several commercial systems are available for the mechanical isolation of SVF, including Lipogems, Lipocell, Mystem, Lipocube Nano, and Lipocube Hybrid. However, there is limited data comparing the effectiveness of these systems in yielding viable cells with optimal regenerative potential. Therefore, this study aimed to compare these five commercial systems in terms of cell yield, cellular activity, and differentiation capacity.
Passivity of gypsum application on soil pH and its significance on exchangeable calcium, available sulphur and yield of paddy, maize and groundnut under different soil conditions
Authors: Prakash Nagabovanalli B. , Dhumgond Prabhudev , Shruthi . , Goiba Pema Khandu , Laxmanarayanan M , GS Yogesh 
Published: June 2023

Abstract
Changes in the pH of acidic, neutral, and alkaline soils in response to various rates and types of gypsum such as slag-based gypsum (SBG) and commercial gypsum (CG), and their effects on soil exchangeable calcium (Ca2+) & available sulphur (S) and yield of different crops were studied in field conditions. Statistical investigation such as linear regression model was used to know the relationship of gypsum application with crop yield revealed that application of SBG and CG was positively and significantly regressed with the yield of paddy, maize and groundnut in all the soils. Significant linear relationship with the pH of acid and neutral soils of paddy have been noticed with application of SBG and CG. Paddy grown in alkaline soil and maize grown in acid and neutral soils had a significant relationship between pH and SBG & CG application. Increased rate of application of SBG as basal and split significantly increased the kernel yield of groundnut. Application of SBG and CG linearly and significantly increased the exchangeable Ca2+ and available S content of soils in which paddy, maize and groundnut were grown. Acid soils responded well in increasing exchangeable Ca2+ and available S compared to other paddy, maize and groundnut soils. Linear relationship was noticed between exchangeable Ca2+ and pH of acid soils paddy, maize and groundnut as influenced by SBG and CG. Whereas, exchangeable Ca2+ content of alkaline soils of paddy also regressed strongly with pH. An increase in available S increased the soil pH of paddy, maize and groundnut, but it was significant with neutral soils of paddy and maize.
HIV Knowledge, Self-Perception of HIV Risk, and Sexual Risk Behaviors among Male Tajik Labor Migrants who inject Drugs in Moscow
Authors: Luc Casey Morgan , Levy Judith , Bakhromov Mahbat , Jonbekov Jonbek , Mackesy-Amiti Mary E. 
Published: June 2023

Abstract
Background: The interplay of HIV knowledge and self-perception of risk for HIV among people who inject drugs is complex and understudied, especially among temporary migrant workers (MWID) who inject drugs while in a host country. In Russia, Tajik migrants make up the largest proportion of Moscow’s foreign labor. Yet, HIV knowledge and self-perceived risk in association with sexual risk behavior among Tajik MWID in Moscow remains unknown.
Objective: This research examines knowledge about HIV transmission, self-perception of HIV risk, and key psychosocial factors that possibly contribute to sexual risk behaviors among male Tajik MWIDs living in Moscow.
Methods: Structured interviews were conducted with 420 male Tajik MWIDs. Modified Poisson regression models investigated possible associations between major risk factors and HIV sexual risk behavior.
Results: Of the 420 MWIDs, 255 men (61%) reported sexual activity in the last 30 days. Level of HIV knowledge was not associated in either direction with condom use or risky sexual partnering, as measured by sex with multiple partners or female sex workers. Higher self-perceived HIV risk predicted less risky sexual partnering, but not condom use. Depression and police-enacted societal stigma were positively associated with risky sexual partnering, while loneliness and depression were associated with condomless sex.
Conclusions: HIV prevention programing for male Tajik MWIDs must go beyond solely educating about factors associated with HIV transmission to include increased awareness of personal risk based on engaging in these behaviors. Additionally, psychological services to counter loneliness, depression, and societal stigma through police harassment are needed.
Improving maternal and child nutrition services in Community based Health Planning and Services zones in the Jirapa municipality of northern Ghana- Challenges and strategies: The perspective of community health officers
Authors: Tengepare Francis Xavier , Chirawurah Dennis , Apanga Stephen 
Published: June 2023

Abstract

Background
The Community-based Health Planning and Services (CHPS) initiative plays a key role in delivering maternal and child health nutrition services in Ghana. This study explored bottle necks associated with delivering maternal and child nutrition services and strategies to address them at the CHPS zones from the perspective of community health workers in rural Northern Ghana.
Methods
An exploratory qualitative cross-sectional study design using key informant interviews involving the municipal nutrition officer and Community Health Officers (CHOs) from eleven CHPS zones was carried out in April 2019. Manual data analysis was done using the framework analysis approach in qualitative data analysis for applied policy research.
Results
Participants identified challenges and strategies to be first: municipal health directorate based which consisted of logistics/medicines; training; supervision/monitoring; and financial support/motivation/incentives and second: CHPS zones based which comprised of planning; home visits; commitment; and community meetings/engagement.
Conclusion
In order to improve maternal and child nutrition services at CHPS zones, there is the need to address certain systemic challenges at both the municipal or district health directorate and CHPS zones levels of the primary health care system. It is therefore recommended that the Ministry of Health, the Ghana Health Service and other stakeholders involved in improving maternal and child nutrition services at the community level actively engage CHOs to help address the systemic challenges.
Probability distributions of model parameters obtained by grid search – application to the geomagnetic anomaly source estimation problem
Authors: Ichihara Hiroshi , Kuwatani Tatsu , Tada Noriko , Nagata Kenji 
Published: June 2023

Abstract
Grid search is widely used in geophysical studies to determine model parameters that explain observed data. The residual between observed data and the model-predicted response, such as root mean square misfit, is usually used as a criterion to select the optimal model parameters. However, root mean square misfit is not a direct criterion for evaluating the reliability of a solution. In this study, we present a method to convert distributions of root mean square misfits obtained from a grid search into probability distributions to evaluate the results statistically based on a Bayesian framework. We applied the proposed method to synthetic geomagnetic anomaly datasets to evaluate the source position and magnitude of magnetic moments. The results are effectively visualized using marginal probability distributions for both well- and ill-posed problems, which are difficult for only root mean square misfits to evaluate. Then we applied the method to real geomagnetic anomaly data reflecting temporal magnetic variations due to volcanic activity in the Nishinoshima volcano. The resultant probability distributions indicate that the source must be in a narrow area northwest of the summit of the volcano with a large magnitude of demagnetization. The method is convenient and thus can be widely applied to multiple geophysical problems, including searches for the source locations of earthquakes, surface geodetic deformation, and magnetic change, and to their joint analyses. In addition, the method easily utilizes previous grid search results to evaluate the probability of model parameters.
Prognostic signature constructed of seven ferroptosis related lncRNAs predicts the prognosis of HBV related HCC
Authors: Wang Wenwen , Wang Lifen , Song Chunxia , Mu Tong , Hu Jinhua , Feng Hua 
Published: June 2023

Abstract

Background
Ferroptosis play crucial roles in cancer. Many lncRNAs were expressed aberrantly and regulated the tumorigenesis and progression of HCC. But the roles of ferroptosis related lncRNAs (FRLncs) in HBV-related HCC (HBV-HCC) remain ambiguous.
Methods
The Cancer Genome Atlas (TCGA) database was applied to achieve gene expression profile and clinical data. The risk signature was constructed by FRLncs based on the univariate and multivariable cox regression analysis. The survival curve, cox regression analysis and time-dependent receiver operating characteristic (ROC) curve was adopted to verify the independence and reliability of the signature. A nomogram was established. Immune infiltrating cells, immune functions and check points were also analyzed.
Results
A risk signature composed of 7 FRLncs (LINC00942, AC131009.1, POLH-AS1, AC090772.3, MKLN1-AS, AC009403.1, AL031985.3) was constructed. The signature divided HBV-HCC patients into high- and low-risk groups. Patients in high-risk group showed a poor prognosis. Even though compared to the stage (areas under curves (AUC) = 0.715), the risk score (AUC = 0.703) seemed not perfect enough, its long-term prediction effect is satisfactory. And the AUC of the risk score for 1-, 3-, 5-year was 0.703, 0.839 and 0.767, respectively. A nomogram composed of gender, stage, age, grade, and risk signature was established. The risk signature and nomogram displayed appreciable independence and reliability in HBV-HCC patients. The endothelial cell, stroma score, hematopoietic stem cell and microenvironment score were expressed differently significantly in the high- and low-risk group according to the XCELL algorithm. PDCD1 and CTL4 were expressed higher in the high-risk group of HCC patients, but no common target was found in the HBV-HCC group.
Conclusion
A 7-lncRNA signature was identified as a potential prognostic predictor for HBV-HCC patients. The current study may help provide new ideas for individualized and precise treatment of HCC, especially HBV-HCC.
Effectiveness of Citrus lemon juice's waste essential oil and aqueous phase as a preservative against Salmonella enteritidis in chicken meat
Authors: Chakoun Hanen , Alabdalall Amira H. , Ababutain Ibtisam , Alyami Reem , Debbabi Ahlem , Yangui Thabèt 
Published: June 2023

Abstract

Through hydro-distillation of industrial lemon juice waste, the study obtainedCitrus lemon's aqueous phase (AP) and essential oil (EO), with the EO yield at approximately 0.89% and a high monoterpene content (50.77% limonene), while the AP contained high total phenols (36.96 ± 1.2 mg g− 1). The EO and AP exhibited Strong bactericidal effects with minimal cidal concentrations values (MCC) ranging from 2.5 to 10 g mL− 1and 187.5 to 1500 g mL− 1, respectively. The inhibitory efficacy of various concentrations of the EO and AP against a foodborne pathogen belonging toSalmonella enteritidisinoculated in chicken meat showed that AP demonstrated a bacteriostatic effect of 0.25–0.5 and 1 mg AP g− 1meat. However, the bacterial count (> 102CFU/25 g meat) rendered it inedible for humans. Additionally, at the maximum concentration (1% v/w), the EO exhibited bactericidal action, but even at the minimum concentration (0.5 v/v EO/v box), the EO vapor action was still the most effective. Sensory analysis indicated that meat treated with the highest concentrations of EO and AP had an unpleasant taste and was discarded. Nonetheless, the study suggests that the AP and EO of lemon juice industrial waste could be a viable alternative to chemical preservatives in the food and pharmaceutical industries.
Depletion of Natural Killer Cells improve cholestasis in a murine model of primary biliary cholangitis
Authors: Liu Bin , Liu Zhaoyang , Liu Qixuan , zhao chenyang , Zang Bo , She Chunhui , yang Yifei , Han Yibing 
Published: June 2023

Abstract
Background and Objective: The aim of this study is to investigate the role of NK cells and their phenotypes in xenobiotic induced murine model of primary biliary cholangitis （PBC）by eliminating NK cells.
Methods: C57BL/6 female mice were immunized with 2OA-BSA and poly I:C to develop the murine model of PBC. Serologic, histologic, and immunologic testing of PBC mice were analyzed by depleting NK cells with ASGM1 before (ASGM1+PBC group) or after immunization (PBC+ASGM1 group). The proportions of NK cells, CD62L+NK cells, CD4+, and CD8+T in liver were analyzed.
Results: ALT, AST and ALP in PBC mice was increased. In contrast to PBC mice, AST in ASGM1+PBC group was decreased. Moreover, TBIL was decreased in PBC+ASGM1 group. Notably, ALP and titer of AMA in mice treated with ASGM1 was decreased when compared with PBC mice. Comparing with HC, ASGM1+PBC or PBC+ASGM1 group mice, the ratio of CD4+T cells in liver declined in PBC mice. In comparison to HC and ASGM1+PBC group mice, the ratio of CD4+/CD8+T cells in PBC mice was lower. The frequency of CD62L+NK cells in the liver of PBC mice increased. However, there was no significant difference in histological scores among ASGM1+PBC, PBC+ASGM1 and PBC mice.
Conclusions: Eliminating NK cells could reduce the degree of cholestasis and improve the immune imbalance. NK cells were functionally involved in pathogenesis and disease progression, especially CD62L+NK cells in liver might aggravate the progression of PBC.
The association between violence against women and chronic pain: A systematic review and meta-analysis
Authors: Uvelli Allison , Ribaudo Carola , Gualtieri Giacomo , Coluccia Anna , Ferretti Fabio 
Published: June 2023

Abstract
Violence against women is still a recurring phenomenon that involves at least 35% of women worldwide. Violence can be sexual, physical, and/or psychological, perpetrated by the partner, another family member, or a stranger. Violence is a public health problem because its consequences include higher morbidity, higher mortality, and short and long-term physical and psychological health diseases. Most studies prove an association between any type of violence and some chronic pain diagnoses but no one has done a complete collection of this evidence. This systematic review and meta-analysis aimed to evaluate whether this association is statistically significant, including the largest number of studies. Through the inclusion of 37 articles, the association has been demonstrated. Compared with no history of violence, women who did experience violence showed increased odds of developing chronic pain. The impact of violence was significant also on fibromyalgia separately, but not on pelvic pain.
Systematic review registration: PROSPERO CRD42023425477
Assessing the causal associations of sleep apnea with mental health and socioeconomic status: a bidirectional two-sample Mendelian randomization
Authors: WU Yuan , LI Zuming , WU Siyi , CHEN XUERU , Chen Hai , Li Jiqiang , Lu Yue , Chen Jiankun , Gan Kao 
Published: June 2023

Abstract
Objective
Traditional observational research has suggested a connection between socioeconomic position, mental health, and sleep apnea (SA), but the specifics of this connection are still unclear. Using the Mendelian randomization approach, we intended to evaluate the potential causal link between mental health, socioeconomic status, and SA.
Methods
Our research employed summary statistics data from large-scale genome-wide association studies (GWAS) on mental health, socioeconomic status, and SA. In the main study, the connection between mental health, socioeconomic status, and SA was examined using the inverse variance weighted approach. In addition, as a supplement, we also used other Mendelian randomization methods, including MR Egger, weighted median, simple mode, and weighted mold.
Results
The primary analysis showed that educational attainment, including longer years of schooling, college or university degree, and higher intelligence was associated with a lower risk of SA (OR = 0.750, 95%CI = 0.653–0.862; OR = 0.558, 95%CI = 0.423–0.735; OR = 0.871, 95%CI = 0.760–0.999, respectively), while social deprivation was associated with a higher risk of SA (OR = 1.821, 95%CI = 1.075–3.085). And the income was not associated with the risk of sleep apnea (OR = 0.877, 95%CI = 0.682–1.129). In mental health exposure, major depressive disorder was associated with a higher risk of sleep apnea (OR = 1.196, 95%CI = 1.015–1.409), while attention-deficit hyperactivity disorder, bipolar disorder, and schizophrenia were not associated with the risk of sleep apnea (OR = 1.064, 95%CI = 0.958–1.181; OR = 1.030, 95%CI = 0.942–1.127; OR = 0.990, 95%CI = 0.957–1.025, respectively). Reverse MR analysis failed to find a causal effect from SA to mental health and socioeconomic status.
Conclusions
This MR investigation offers proof of a possible causal relationship between SA, socioeconomic level, and mental health.