bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alteration of Ascending Colon Mucosal Microbiota in Patients after Cholecystectomy
Authors: Fan Miao-Yan , Lu You , Cui Meng-Yan , Zhao Meng-Qi , Wang Jing-Jing , Jiang Qiao-Li , Lu Ying-Ying 
Published: August 2023

Abstract
BACKGROUND
Cholecystectomy is an effective therapy for gallstones, however, the incidence of CRC has increased significantly in post-cholecystectomy (PC) patients. Whether it is related to the changed mucosal microbiota in ascending colon is still unclear.
AIM
To explore the association between gut microbiota and cholecystectomy.
METHODS
Mucosal biopsy samples were collected from 30 PC patients (the test group) with gallbladder stones and 28 healthy individuals (the control group) by colonoscopy. Subsequently, the test group was subdivided into the YMA group or SNR group(age over or under 60), DG group or NG group (with or without diarrhea) and Log group or Sht group(duration over or under 5 years) according to patients’ clinical characteristics. 16S-rRNA gene amplicon sequencing was performed and alpha diversity, beta diversity and composition analysis were determined. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States based on the Kyoto Encyclopedia of Genes and Genomes database was used to predict the function of the microbiome.
RESULTS
The PC patients showed similar richness and overall composition with healthy controls, but PC patients over 60 years showed a different structure than those under 60 years. At the phylum level, the richness of Bacteroidetes was significantly higher in PC patients. Similarly, the genus Bacteroides, Parabacteroidesand Bilophila were remarkably more abundant in PC patients compared with the controls. In addition, the PC patients had significant enrichments in both metabolic pathways, including Lipopolysaccharide and vancomycin group antibiotics biosynthesis compared to the controls.
CONCLUSION
Our study suggested that mucosal microbiota was changed in PC patients, which may reveal new insight into therapeutic options for colorectal cancer and diarrhea after cholecystectomy.
Identification of molecular subtypes based on histone arginine methylation in bladder cancer
Authors: Guo Xiaobin , Li Jinrong , Liang Zhongxia , Wu Fulin , Lin Junhao , Huang Guihai , Li Yue , Li Ximing , Chen Zhi , Hao Nan , Li Wei 
Published: August 2023

Abstract
Background
​Histone arginine methylation is an important feature in bladder cancer that affects prognosis and therapeutic response. However, the investigation of molecular subtypes based upon histone arginine methylation is still being exploited.
Methods
From the TCGA database, we obtained the RNA sequencing and somatic mutation results of 412 bladder cancer patients. To identify molecular subtypes associated with histone arginine methylation, consensus clustering was performed. These histone arginine methylation-associated subtypes were compared in terms of prognosis, clinical features, immune cell infiltration, and somatic mutation profiles. Moreover, we utilized the CTRP and GDSC databases to examine how drug sensitivity is related to mRNA expression of HAM-related genes.
Results
Based on our findings, bladder cancer could be divided into histone arginine methylation-low (HAM-low) and histone arginine methylation-high (HAM-high) subtypes with distinctive clinicopathological characteristics, tumor microenvironment and prognostic. There is a dismal prognosis associated with high histone arginine methylation subtypes and a high frequency of oncogene mutations in these subtypes. Conversely, histone arginine methylation-low subtypes are associated with a better clinical outcome and a lower frequency of oncogene mutations. There was a correlation between the HAM-high subtype and the immune-hot phenotype, while the HAM-low subtype was associated with the immune-cold phenotype. As well, we develop and validate a prognostic model associated with histone arginine methylation that has good predictive power. The results of drug sensitivity tests revealed that HAM-related genes are associated with resistance to most drugs. A positive correlation was found between XAV939 or Cetuximab and HAM-related gene expression, suggesting that these agents may be beneficial to patients who fall into the HAM-high subgroup of BC patients.
Conclusion
​In summary, we have established a novel bladder classification based on histone arginine methylation subtypes. This classification has significant consequences for the estimation of prognosis and drug sensitivity, as well as the tumor microenvironment.
Metagenomic Investigations on Antibiotic Resistance and Microbial Virulence in Oil-Polluted Soils from China
Authors: Qaria Majjid A , Xu Chunyan , Hussain Arif , Nawaz Muhammad Zohaib , Zhu Daochen 
Published: August 2023

Abstract
Engine oil spills have been associated with a wide range of human health problems. However, little is known about the effects of petroleum hydrocarbon pollution on soil microbial communities. In this study, three samples were collected from oil-polluted soils (OPS) and one control soil (CS), from Taolin town, China, near the old engine’s scrapes were used. The aims of this study were to conduct metagenomic sequencing and subsequently perform resistome and Virulome analysis. We also aimed to validate antimicrobial resistance and virulence genes and antibacterial sensitivity profiles among the isolates from oil polluted soils. The OPS microbial community was dominated by bacterial species compared to the control samples which were dominated by metazoans and other organisms. Secondly, the resistosome and virulome analysis showed that ARGs and virulence factors were higher among OPSs microbial communities. Antibiotic susceptibility assay and qPCR analysis for ARGs and virulence factors showed that the oil polluted soil samples had remarkably enhanced expression of these ARGs and virulence genes. Our study suggests that oil pollution contributes to shifting microbial communities to more resilient types that could survive the toxicity of oil pollution and subsequently became more resilient in terms of higher resistance and virulence potential.
Dust events control nitrogen cycling and nitrate/(per)chlorate relationships on Mars
Authors: Fu Xiaohui , Mao Wenshuo , Wu Zhongchen , Zhang Jiang , Ling Zongcheng , Liu Yang , Zhao Yu-Yan , Liu Jiacheng , Cui He , Changela Hitesh , Ni Yuheng , Yan Fabao , Michalski Joseph 
Published: August 2023

Abstract
Nitrogen is an important part of any biosphere/organic cycling, but aspects of the nitrogen cycle for Mars are quite unclear. Nitrate and (per)chlorate have been detected in sedimentary rocks and aeolian sediments in Gale crater on Mars as well as in martian meteorites. Their occurrences have implications for global nitrogen/chlorine cycles and the habitability on Mars. However, the origins of nitrate and (per)chlorate, and their possible formation are not well understood. Interaction between the atmosphere and surface of Mars during dust events have cause electrostatic discharge (ESD). By simulating this process in a Mars chamber, nitrate and (per)chlorate can form from halite precursors under martian atmospheric conditions. The obtained nitrate/(per)chlorate ratio (0.28 ± 0.19) is in agreement with Gale crater observations (0.23 ± 0.12), which is much lower than in terrestrial Mars analogues and the products of other pathways proposed for Mars. The electrochemistry during Mars dust events represents a new nitrogen fixation mechanism, which may also act as a crucial part of global nitrogen cycling on present-day Mars even throughout the whole Amazonian period. These nitrate salts in martian dusts potentially provide an accessible source of nitrogen for possible organic molecule formation on Mars.
Selection, characterization and in vivo evaluation of novel CD44v6-targeting antibodies for targeted molecular radiotherapy
Authors: Mortensen Anja Charlotte Lundgren , Berglund H. , Segerström L. , Hofström C. , Persson H. , Nygren P-Å. , Nilvebrant J. , Frejd F. , Nestor M. 
Published: August 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Factors influencing the postoperative flexion angle in cruciate-sacrificing rotating platform of total knee arthroplasty
Authors: Matsuoka Daisuke , Inagaki Yusuke , Mawarikado Yuya , Ogawa Munehiro , Seko Munehito , Fujii Tadashi , Tanaka Yasuhito 
Published: August 2023

Abstract

Background
Various factors affect the improvement of range of motion (ROM) after total knee arthroplasty (TKA). However, there are few reports specific to cruciate-sacrificing rotating platform (CSRP) TKA. In this study, factors affecting postoperative ROM improvement of CSRP TKA were investigated.
Methods
The study included 79 patients with knee osteoarthritis who underwent unilateral CSRP TKA at our institution. The group with an improvement of 5 degrees or more (Δflexion angle) than the preoperative was defined as the good Δflexion group (38 knees), and that with less than 5 degrees was defined as the poor Δflexion group (41 knees). The assessments were performed one day and one year after surgery. Factors including rest and walking pain, knee flexion and extension angle, isometric knee extension strength, the five subscales of KOOS, α, β, γ and δ angles, FTA, and condylar twist angle were assessed. Unpaired t-test, Mann–Whitney U test, and chi-square test were used to test differences between the good and poor Δflexion groups. Multiple logistic regression examined the association between each factor and the dependent variables (good Δflexion or poor Δflexion).
Results
Significant differences in the preoperative knee flexion, postoperative knee flexion, preoperative knee extension, and postoperative knee extension angles, postoperative KOOS pain and activity of daily living, β, ɤ angles were observed between the good and poor Δflexion groups. The model chi-squared test revealed that the ɤ angle was significantly affected with Δ flexion angle.
Conclusions
With the CSRP TKA, flexion insertion of the femoral component was associated with postoperative flexion ROM improvement.
RGB-D Salient Object Detection Based on Feature Extraction and Integration Convolutional Capsule Network
Authors: Xu Kun , Guo Jichang 
Published: August 2023

Abstract
Fully Convolutional Neural Networks (FCNs) have shown advantages in the salient object detection (SOD) by using the RGB images or RGB-D images. However, there is a object-part dilemma since most FCNs inevitably lead to an incomplete segmentation of the salient object. Although the Capsule Network(CapsNet) is capable of recognizing a complete object which has better performance, it is highly computational demanding and time consuming. In this paper, we propose a novel Feature Extraction and Integration Convolutional Capsule Network(FEICaps) based on Convolutional Capsule Network for dealing with the object-part relationship, with less computation demanding. First and foremost, RGB features are extracted and integrated by using the VGG backbone and feature extraction module. Then, these features, integrating with depth images by using Feature Depth Module(FDM), are upsampled progressively to produce a feature map. In the next step, the feature map is fed into the Feature-integrated convolutional Capsule Network(FiCaps) to explore the object-part relationship. The FiCapsextracts object-part features by using convolutional capsules with locally-connected routing and predicts the final salient map based on the deconvolutional capsules. Experimental results on four RGB-D benchmark datasets show that the proposed method outperforms 23 state-of-the-art algorithms.
High temperature dielectric breakdown and energy storage properties of polyetherimide nanocomposites improved by hindering molecular motion
Authors: yang lingyu , Min Daomin , Gao Ziwei , Yang Liuqing , Zhu Yuanwei , Liu Wenfeng 
Published: August 2023

Abstract
Polyetherimide (PEI) has excellent thermal and electrical properties, and is widely used as a dielectric material for high-temperature high-power film capacitors in power systems, new energy vehicles and other fields. However, as the operating temperature increases, the electrical conductivity increases and the breakdown strength decreases, reducing the energy storage density of capacitors significantly and limiting the practical applications. To clarify the influencing mechanism of high temperature on breakdown properties and energy storage performance of dielectrics, this paper establishes a charge transport and molecular displacement modulated (CTMD) breakdown model based on the expansion movement of molecular chain segments to investigate change regularity in charge transport and molecular chain motion of PEI nanocomposites (PNCs) at high temperatures. The results show that at high temperatures of 100°C, PEI PNCs with an appropriate nanoparticle content (3wt%) show a 5.35% reduction in maximum internal temperature, a 28.79% reduction in maximum molecular displacement and an 11.20% increase in breakdown strength compared to pure PEI. Nano-doping can effectively increase the difficulty of molecular segment motion, thereby reducing the excitation volume in which they provide energy for charge transport. Thus, charge transport is inhibited, current density is reduced and excess Joule heat is avoided. Eventually, high temperature dielectric breakdown and energy storage properties of PEI PNCs can be significantly improved.
Understanding vulnerability through variability: a longitudinal twin study linking sex differences in neurodiversity, neurodevelopment and X-linked genetic mechanisms.
Authors: Wierenga Lara , Zabihi M. , Drunen Lina van , Meulen Mara van der , Achterberg Michelle , Rutherford Sage , Marquand Andre , Crone Eveline A. 
Published: August 2023

Abstract
Background: There are marked sex/gender differences in the prevalence and expression of neurodiversity. Yet, it is unclear how sex/gender related mechanisms may affect differences in expression of symptoms. In the present study we test whether previously reported greater male than female variability in neuroanatomy is related to sex differences in X-linked mechanisms and may explain behavioral and cognitive variation in neurodiversity. Method: The present study included a longitudinal twin study (N=990 twins; 56% monozygotic, 7-14 yo). Results: Greater male than female variability was most pronounced in structure and development of cortical surface area. Twin modeling results support a significant role of X-chromosome expression related to neuroanatomical variation. In addition, normative modeling analysis, showed that deviations in brain anatomy were associated to neurodiverse behavior. Conclusions: These findings together highlight the importance of studying sex and gender linked mechanisms in the brain and mental illness beyond mean group modeling.
Quality of Life in the Community Versus Rehabilitation Hospital Setting During COVID-19 Quarantine in Shanghai, China.
Authors: Ferland Amanda , Zhang Zhe’An , Sun Tian’Bao , Bai Chen’Yuan , Ma Xia , Geng Su’Ping 
Published: August 2023

Abstract
Aims The mutated strain of the new coronavirus, the OMICRON virus, began to spread in Shanghai in early April 2022. The government adopted a quarantine policy that lasted for two months. This study is looking at the effect that this specific time period had on mental health and quality of life (QOL) for the people of Shanghai 2022.
Methods Patients, caregivers, and community members who did not suffer from Covid-19, but did experience the closure of the city, completed 281 valid questionnaires either online or on paper within one week of unblocking. Questionnaires included 9-item Patient Health Questionnaire (PHQ-9), 7-item Generalized Anxiety Disorder (GAD-7), and the Short Form-12 (SF-12).
Results 281 valid questionnaires were completed; females 111, males 170, with an age range of 14 to 92,52.32 ± 16.26. Gender and subject’s education had no significant effect on depression or anxiety. For source, more patients in the rehabilitation setting scored＞9 on PHQ and GAD questionnaires (PHQ 28%, GAD 16%) than the other 2 groups, and a significant difference was found in PHQ score (p value=0.032), while the GAD difference was not significant (p value=0.339). For age, more participants from group 1 (age 19-39) scored ＞9 on PHQ and GAD questionnaires (PHQ 29%, GAD 16%) compared to the other 2 groups. A significant difference was found in PHQ score (p value=0.022), while the GAD difference was not significant (p value=0.392). Regarding populations, there was also significant differences in PCS12 scores between patients and community members, p value <0.0001; patients and caregivers have significant differences, p value <0.0001; caregivers and community members have no significant differences, p value is 0.9434. There is significant difference in PCS12 scores between patients and caregivers, p value < 0.0001. Regarding age, group 1 (age 19-39) and group 3 (age 60+) have significant difference in PCS12 scores, p value < 0.0001; and group 1 and group 2 (age 40-59) have no significant difference, with the p value 0.3903.
Discussion/Conclusion This cross-sectional study looking at quality of life in three different populations of Shanghai residents during Covid-19 lockdown demonstrated no significant differences in overall QOL between people living in the community versus those living in a rehabilitation hospital setting. There were significant differences specifically in physical functional scores (PCS12) and depression scores (PHQ-9) between patients in the rehabilitation setting when compared to their caregivers or community members. Patients demonstrated decreased physical functioning and increased levels of depression. More surprisingly, there were differences in depression and anxiety scores between age groups in all populations, with younger Shanghai residents demonstrating increased levels of depression and anxiety when compared to older residents following lockdown.
Mitochondrial SIRT3 as a Protective Factor against Cyclosporine A-Induced Nephrotoxicity
Authors: Kim Ji Eun , Jo Min Jee , Bae So Yeon , Ahn Shin Young , Ko Gang Jee , Kwon Young Joo 
Published: August 2023

Abstract

Sirtuin3 (SIRT3), a mitochondrial deacetylase, has been shown to be involved in various kidney diseases. In this study, we aimed to clarify the role of SIRT3 in cyclosporine-induced nephrotoxicity and the associated mitochondrial dysfunction. Madin-Darby canine kidney (MDCK) cells were transfected with Flag-tagged SIRT3 for SIRT3 overexpression or SIRT3 siRNA for the inhibition of SIRT3. Subsequently, the cells were treated with cyclosporine A (CsA) or vehicle. Wild-type and SIRT3 knockout (KO) mice were randomly assigned to receive cyclosporine A or olive oil. Our results revealed that CsA treatment inhibited mitochondrial SIRT3 expression in MDCK cells. Inhibition of SIRT3 through siRNA transfection exacerbated apoptosis, impaired the expression of the AMP-activated protein kinase-peroxisome proliferator-activated receptor gamma coactivator 1 alpha (AMPK-PGC1α) pathway, and worsened mitochondrial dysfunction induced by CsA treatment. Conversely, overexpression of SIRT3 through Flag-tagged SIRT3 transfection ameliorated apoptosis, increased the expression of mitochondrial superoxide dismutase 2, and restored the mitochondrial regulator pathway, AMPK-PGC1α. In SIRT3 KO mice, CsA treatment led to aggravated kidney dysfunction, increased kidney tubular injury, and accumulation of oxidative end products indicative of oxidative stress injury. Overall, our findings suggest that SIRT3 plays a protective role in alleviating mitochondrial dysfunction caused by CsA through the activation of the AMPK-PGC1α pathway, thereby preventing further kidney injury.
A prediction model of sperm retrieval in males with idiopathic non-obstructive azoospermia for microdissection testicular sperm extraction
Authors: Zheng Yi , Li Ding-Ming , Jiang Xiao-Hui , Bai Heng-Zhou , Zhao Gui-Cheng 
Published: August 2023

Abstract
Patients with Idiopathic non-obstructive azoospermia (iNOA) can achieve fertility by extracting testicular sperm through microdissection testicular sperm extraction (mTESE). But more than half of iNOA patients still cannot benefit from mTESE. In recent years, some studies had reported that serum hormones may be related to the outcome of sperm retrieval, but few had been verified. We hope to obtain a predictive method that is convenient for clinical application and can help judge the outcome of sperm extraction before implementing mTESE. We performed a retrospective analysis of NOA patients who underwent mTESE in the same andrology center from June 2020 to November 2022. A total of 261 patients with complete data were collected, logistic regression analysis was performed and a predictive model was constructed. Then, from December 2022 to May 2023, one prospective cohort of 48 NOA patients who met the inclusion criteria from the same center, were recruited to validate the risk prediction model. We successfully constructed a logistic regression model to predict the outcome of iNOA patients undergoing mTESE and found that higher serum anti-mullerian hormone (AMH) levels were associated with failure sperm retrieval, resulting in an AMH cut-off of 2.60 ng/ml. The area under the receiver operating curve was 0.811, the sensitivity was 0.870, and the specificity was 0.705. Decision curve analysis demonstrated that the threshold probability was above 4%, and unnecessary mTESE could be reduced using this model. In a prospective cohort at the same center, 85.42% (41/48) of iNOA patients correctly identified the mTESE outcome using this model. A logistic regression model with AMH as an independent predictor can predict mTESE outcomes in iNOA patients. Preoperative selection of mTESE in patients with iNOA using this model had clinical benefit in reducing unnecessary surgery. The model demonstrated good accuracy in a small prospective cohort validation.