bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship between serum uric acid-creatinine ratio and risk factors of cerebral Hemodynamics and stroke in physical examination population
Authors: Liu Dongxia , Dong Xunan , Jiang Yan , Zhu Yu , Wang Yushan 
Published: May 2023

Abstract
Background The serum uric acid to creatinine ratio (SUA/Cr) is important in cardiovascular diseases. The aim of this study was to investigate the correlation between SUA/Cr ratio and cerebral hemodynamics and to analyze the relationship between different levels of SUA/Cr ratio and risk factors associated with stroke.
Methods A population of 12,785 middle-aged and elderly medical checkups who completed CVHI testing at the First Affiliated Hospital of Xinjiang Medical University from June 2020 to May 2021 was selected for the study. The medical examination population was divided into Q1, Q2, Q3 and Q4 groups according to the quartiles of SUA/Cr ratio. The relationship between the SUA/Cr ratios and CVHI and stroke related risk factors in the middle-aged and elderly population was analyzed.
Results A total of 12785 cases were included in the study, with an age range of 35 to 91 years and a mean of (50.64 ± 10.12) years. In the SUA/Cr grouping, the differences in the composition ratios of the population by gender and age were significant (P < 0.05), and the composition ratios of the population with abnormal systolic blood pressure, diastolic blood pressure, BMI, and history of alcohol consumption, hypertension, diabetes mellitus, and hyperlipidemia in the SUA/Cr grouping showed statistical significance (P < 0.05). the composition ratios of the group with abnormal blood pressure, alcohol consumption, history of hypertension, history of diabetes mellitus, and hyperlipidemia in the Q4 group were significantly higher than those of the other groups (P < 0.05), The composition ratios of the group with abnormal blood pressure, alcohol consumption, history of hypertension, history of diabetes mellitus, and history of hyperlipidemia were all significantly higher than those of the other groups (P < 0.05). As the ratio of SUA/Cr increases, the CVHI integral value will show a corresponding decreasing trend.
Conclusions There is a correlation between SUA/Cr and CVHI, which should be given high priority.
O+ density distribution in the nightside ionosphere reconstructed from ISS-IMAP/EUVI data
Authors: Nakano Shin'ya , Hozumi Yuta , Saito Akinori , Yoshikawa Ichiro , Yamazaki Atsushi , Yoshioka Kazuo , Murakami Go 
Published: May 2023

Abstract
The O+ density distribution in the nightside ionosphere has been reconstructed from extreme ultraviolet (EUV) images taken by the EUVI-B imager of the International Space Station Ionosphere, Mesosphere, upper Atmosphere, and Plasmasphere mapping (ISS-IMAP) cameras. The EUVI-B imager covers the wavelength range from about 70 nm to 110 nm and mainly observes the 91.1 nm emission from the recombination of O+ ions and electrons. Assuming that the electron density is equal to the O+ density in the F-region where the imager observes, the EUV intensity observed by EUVI-B is approximately proportional to the line-of-sight integral of the square of the O+ density. This enables us to estimate the O+ density distribution in the F-region from a sequence of EUVI-B data in each International Space Station (ISS) orbit with a Bayesian method. We demonstrate the reconstruction of the O+ distribution. In particular, the O+ density structure of the equatorial anomaly (EA) in the vicinity of an ISS orbit is obtained.
A Multispecies Competitive Nanobody-Based Elisa for the Detection of Antibodies Against Hepatitis E Virus
Authors: Arce Lorena Paola , Pavan María Florencia , Bok Marina , Gutierrez Silvina Elena , Santos Agostina Tammone , Condorí Walter Ezequiel , Uhart Marcela María , Estein Silvia Marcela , Parreño Viviana , Vizoso-Pinto María Guadalupe , Ibañez Lorena Itatí 
Published: May 2023

Abstract
The hepatitis E virus (HEV) is an emergent zoonotic virus causing viral hepatitis worldwide. Clinically, hepatitis E is not easily distinguished from other types of acute viral hepatitis. There is a need for HEV diagnostic assays to detect and prevent interspecies transmission among susceptible populations. Nanobodies (Nbs) are expressed recombinantly in different systems, produced with high yields, and have superior physicochemical properties compared with conventional antibodies (Ab). Several Nbs against ORF2, the capsid protein and main antigen, were selected and produced in E. coli. Nb39 and Nb74 specifically recognized HEV ORF2 (genotypes 3 and 4). A competitive ELISA (cELISA) was developed and validated using a reference panel of human (n = 86) and swine sera (n = 116) tested in comparison with a commercial kit. The optimal cutoff values determined by ROC analysis were 69.16% (human) and 58.76% (swine); the sensitivity and specificity were high: 97.4% (IC95%; 86.5–99.5%) and 95.8% (IC95%; 86.0-98.8%) for human vs. 100% (IC95%: 93.5–100%) and 98.3% (IC95%: 91.0-99.7%) for swine. Further, the cELISA detected total anti-HEV antibodies in wild boar, deer, and mice. To our knowledge, this is the first report of production of Nbs against HEV-3 ORF2 for diagnostic purposes.
Producing hydrogen from electricity: how modeling additionality drives the emissions impact of time matching requirements
Authors: Schittekatte Tim , Cybulsky Anna , Giovanniello Michael , Mallapragada Dharik 
Published: May 2023

Abstract
The United States’ Inflation Reduction Act (IRA) includes a hydrogen (H2) production tax credit (PTC) to subsidize low-carbon H2 up to $3/kg. It is hard to quantify the emissions impact of electrolyzers using grid electricity and contracting “additional” grid-interconnected renewable electricity; the H2 producer offtakes its electricity supply from newly installed low-carbon generators that are not co-located with the electrolyzer. Recent research offers conflicting guidance on qualifying time-matching requirements, which we find can be explained by differences in the modeling of additionality requirements. One approach considers any resources that are not operating prior to the installation of the electrolyzer to be additional — i.e., H2 and non-H2 electricity demand “compete” for entering renewables. Another approach enforces a stricter definition of additionality by only considering low-carbon electricity supply that would otherwise not be deployed without H2 production to be additional — we call this a “non-compete” framework. We model both approaches for case studies of the Texas and Florida grids and confirm that the additionality framework drives the consequential emissions impact of H2 production. We estimate significantly less consequential emissions under an annual time matching requirement in the “non-compete” framework. Introducing an upper limit to the electrolyzer’s capacity factor can reduce consequential emissions with annual time-matching under the “compete” framework. We argue that because the demand for electrolytic H2 is still relatively small, today’s context more closely resembles a “non-compete” framework and, thus, a low consequential emissions impact with annual time-matching is likely. However, as demand for electrolytic H2 grows, the risk of a higher consequential emissions impact increases under annual time-matching, as the paradigm shifts to resemble the modeled “compete” framework. Hence, we argue for a “phased approach” in the requirements for the attribution of the PTC: annual matching in the near term with a re-evaluation leaning towards hourly matching later on in the decade.
Human Platelet Lysate stimulates neurotrophic properties of Human Adipose-Derived Stem Cells better than Schwann Cell-like Cells
Authors: Brambilla Stefania , Guiotto Martino , Torretta Enrica , Armenia Ilaria , Moretti Matteo , Gelfi Cecilia , Palombella Silvia , Summa Pietro G. di 
Published: May 2023

Abstract
Background: Trauma-associated peripheral nerve injury is a widespread clinical problem causing sensory and motor disabilities. Schwann cells (SCs) contribute to nerve regeneration, mainly by secreting nerve growth factor (NGF) and brain-derived neurotrophic factor. In the last years, adipose-derived stem cells (ASCs) differentiated into SCs (SC-ASCs) were considered as promising cell therapy. However, the cell trans-differentiation process has not been effectively showed and presents several drawbacks, thus an alternative approach for increasing ASCs neurotrophic properties is highly demanded. In the context of human cell-based therapies, Good Manufacturing Practice directions indicate that FBS should be substituted with a xenogeneic-free supplement, such as Human Platelet Lysate (HPL). Previously, we demonstrated that neurotrophic properties of HPL-cultured ASCs were superior compared to undifferentiated FBS-cultured ASCs. Therefore, as following step, here we compared the neurotrophic properties of differentiated SC-like ASCs and HPL-cultured ASCs.
Methods: Both cell groups were investigated for gene expression level of neurotrophic factors, their receptors and neuronal markers. Moreover, the expression of nestin was quantitatively evaluated by flow cytometry. The commitment towards the SC phenotype was assessed with immunofluorescence pictures. Proteomics analysis was performed on both cells and their conditioned media to compare the differential protein profile. Finally, neurotrophic abilities of both groups were evaluated with a functional co-culture assay, assessing dorsal root ganglia survival and neurite outgrowth.
Results: HPL-cultured ASCs demonstrated higher gene expression of NGF and lower expression of S100B. Moreover, nestin was present in almost all HPL-cultured ASCs and only in one quarter of SC-ASCs. Immunofluorescence confirmed that S100B was not present in HPL-cultured ASCs. Proteomics analysis validated the higher expression of nestin and the increase of cytoskeletal and ECM proteins involved in neural regeneration processes. The co-culture assay highlighted that neurite outgrowth was higher in the presence of HPL-ASCs or their conditioned medium compared to SC-ASCs.
Conclusions: All together, our results show that HPL-ASCs were more neurotrophic than SC-ASCs. We highlighted that the HPL triggers an immature neuro-induction state of ASCs, while keeping their stem properties, paving the way for innovative therapies for nerve regeneration.
Identification of the Shared Gene Signatures and Biological Mechanism in Recurrent Implantation Failure and Decreased Embryo Quality Undergoing In Vitro Fertilization
Authors: Huang Jingyu , Tan Yong , Liu Kailu , Liu Li , Gao Mengya , Xia Guicheng , Nie Xiaowei 
Published: May 2023

Abstract

Aim To identify the shared gene signatures and biological mechanism in recurrent implantation failure and decreased embryo quality undergoing in vitro fertilization.Methods Relevant microarray data were selected from the GEO to screen genes that differentially expressed in response to decreased embryo quality and RIF.Results We discovered upregulated and downregulated genes. Simultaneously, we identified hub genes associated with decreased embryo quality and RIF. GO and KEGG analysis results revealed a high correlation with hypoxia-induced, inflammatory, cell-cell junction, and ubiquitin ligase complex. HIF1A, Jun, SP1, SIRT1, and POU2F1, considered TFs for decreased embryo quality and RIF.Conclusion This study explored the molecular mechanisms underlying these features and screened potential biological targets associated with decreased embryo quality and RIF.
Face Mask Reduces Gaze-Cueing Effect
Authors: Jia Han , Wang Qi , Feng Xinghe , Hu Zhonghua 
Published: May 2023

Abstract
Recent studies have found that face masks affect social cognition and behaviour in the context of the novel coronavirus (COVID-19) pandemic. The eyes, the only part of the face not covered by face masks, are an important spatial attention cue that can trigger social attention orienting. Here, we adopted a spatial gaze-cueing task to investigate whether face masks affect social attention orienting triggered by eye gaze cues. In Experiment 1, participants were asked to determine the orientation of a target line under two types of cues—masked and non-masked faces—and two stimulus onset asynchrony (SOA) conditions (300 ms and 1000 ms). The results showed that masked faces induced a smaller gaze-cueing effect (GCE) compared to non-masked faces at 300 ms SOA, while two face types induced similar GCEs at 1000 ms SOA. Experiment 2 used mouth-obscured faces and non-masked faces as cues and found that no significant difference in GCE between the two types at either 1000 ms or 300 ms SOA, indicating that the reduction of GCE caused by the masked face was due to the social meaning expressed by the mask rather than a physical effect of masking. The present study extends previous findings to support the idea that high-level social information affects the processing of eye gaze direction and provides evidence that face masks affect social cognition and behaviour in the context of COVID-19.
High-throughput Sequencing-based Analysis of Microbial Diversity in Daihai Lake
Authors: Li Xing , Chen Xiaojiang , Li Jiajia 
Published: May 2023

Abstract
Purpose The bacterioplankton community is an important part of the lake ecosystem, which not only influences the physical and chemical environmental factors of lake water quality, but also affects the functional processes of the lake ecosystem. However, little is known about the formation and maintenance mechanisms of bacterial plankton diversity and community structure composition in the inland saline-alkali lakes.
Methods In this study, the inland saline-alkali lake Daihai was used as the object of study, 9 sampling areas were set up according to the actual situation of Daihai Lake, and 16S rRNA Illumina Miseq™/Hiseq™ high-throughput sequencing technology was applied to study the spatial distribution characteristics of bacterial microbial diversity and community structure composition of lake water bodies and their key influencing factors.
Results The bacterioplankton community composition and the relative abundance of each group of bacteria in 9 sample areas of Daihai Lake were high, with the main bacterial taxa at the phylum level being Actinobacteria, Bacteroidetes, Cyanobacteria_Chlorophyceae, Bacteroides, Cyanobacteria_Chloroplast, Bacteroidetes, Firmicutes, unclassified_Bacteria, Proteobacteria and Verrucomicrobia, with a maximum value of 4.14 (DH5) and a minimum value of 3.53 (DH3) for the bacterial community diversity index. Proteobacteria at the phylum level and Sulfurimonas at the genus level were sensitive to the evolution of lake degradation; the water quality physicochemical parameters DO (dissolved oxygen), pH, con(conductivity), TP (total phosphorus), sal(salinity) and TN (total nitrogen) had the most significant effect on the overall bacterial community structure with the highest correlation coefficient, and the Mantel test showed that this correlation was significant.
Conclusion The bacterioplankton community structure of the inland saline-alkali lake Daihai is highly variable, and the genus Sulfurimonas plays an important role in the biogeochemical cycling of Daihai Lake waters and should receive more attention in future studies.
Peripheral nervous system involvement in SSPE as a parainfectious manifestation - A case report
Authors: Nangla Parnika , Bansal Bhavik , George Jerry A , Singh Mamta Bhushan , Das Sumanto , Sharma Mehar C , Suri Vaishali , Brijwal Megha , Garg Ajay , Tripathi Manjari , Vibha Deepti , Singh Rajesh Kumar , Elavarasi Arunmozhimaran 
Published: May 2023

Abstract
Background
Rapidly progressive encephalopathy in a young adult has a range of differentials requiring an exhaustive workup. We present a man with a history of encephalopathy with neuropathy and a rare diagnosis.
Case presentation
A 40 year-old man presented with a 6-month history of cognitive dysfunction and an asymmetric gradually progressing ascending quadriparesis with the involvement progressing in the order of motor, sensory, autonomic and bulbar. MRI of the brain showed diffuse atrophy. Nerve conduction studies revealed sensorimotor neuropathy affecting all four limbs. CSF showed a negative autoimmune panel and no signs of an infectious etiology. The patient was started on plasma exchange with a provisional diagnosis of an immune mediated disorder.
A nerve biopsy showed axonolysis and demyelination. EEG showed delta wave slowing and periodic discharges. A possibility of SSPE was considered, although peripheral neuropathy is an atypical presenting feature, following which the measles IgG antibodies titers in CSF and serum were found to be raised at 377.2 U/mL and 197 U/mL respectively. He had persistent sepsis and several episodes of respiratory distress requiring invasive mechanical ventilation. He was started on intrathecal interferon with a period of objective stability after which the sensorium gradually deteriorated and the outcome was fatal.
Conclusions
Subacute sclerosing panencephalitis (SSPE) is a progressive disorder caused by a persistent defective measles virus and causes death several years after the measles infection. It is suspected based on characteristic clinical features, EEG findings and demonstration of measles antibodies in CSF. The diagnosis is made based on Dyken's criteria. SSPE can present in an atypical fashion with peripheral nerve involvement. Neuropathy may occur due to para-infectious demyelination with no cellular infiltration seen on the nerve biopsy. A high index of suspicion aids diagnosis in rarer presentations.
DNA barcoding, aggressiveness of Bipolaris sorokiniana isolates, and pathogenicity of emerging B. gossypina in barley in subtropical southern Brazil
Authors: Rodrigues Paula Cristina dos Santos , Angelotti-Zampar Priscila , Feksa Heraldo Rosa , Seron Giovanna , Tessmann Dauri José 
Published: May 2023

Abstract
Southern Brazil has experienced severe outbreaks of leaf blotch disease in barley, which have led to reduced grain quality and yield. A field survey was conducted in the major barley-producing areas of Paraná state using DNA barcoding techniques to identify Bipolaris sorokiniana isolates, the causative agent of spot blotch, aiming to determine the extent of pathogenic variability among the isolates. DNA barcoding and phylogenetic analyses were based on internal transcribed spacers 1 and 2 with the 5.8S region (ITS) of rDNA, glyceraldehyde-3-phosphate dehydrogenase (gapdh), and translation elongation factor 1-α (tef1) genes. Out of the 124 isolates examined, which were collected from 20 commercial barley fields each year in 2020 and 2021, 116 isolates were identified as B. sorokiniana and eight isolates as B. gossypina. Koch's postulates confirmed the pathogenicity of B. gossypina in barley, representing a novel occurrence worldwide. Previously, this pathogen was found only in cotton (Gossypium sp.) in Kenya. The fungus causes the development of elongated brown lesions surrounded by irregular yellow halos, starting at minute points. Subtle differences between these symptoms and spot blotch caused by B. sorokiniana are discussed. This study also assessed the aggressiveness of 16 B. sorokiniana isolates on potted barley grown under controlled conditions, using a visual infection rate (IR) scale ranging from 1 to 9. Significant differences in aggressiveness were recorded among the isolates, with the IR ranging from 5.1 to 7.4 in the cultivar ANA03 and 5.7 to 8.1 in the cultivar Imperatriz. The interaction between the isolates and cultivars was not significant. These findings could support breeding programs aiming to develop cultivars with genetic resistance to spot blotch disease in Brazil.
Ground deformations associated with an overpressurized hydrothermal systems at Azuma volcano (Japan) revealed by InSAR data
Authors: Himematsu Yuji , Ozawa Taku 
Published: May 2023

Abstract
Inflations at active volcanoes are indicators of overpressure in the subsurface, which is known to be a phenomenon that precedes eruptions. Volcanic overpressure is induced by the injection of magmatic fluids, accumulated magma, or heat supply from greater depths. Azuma volcano (Japan) has experienced several episodes of volcanic unrest with increase in seismicity at the depth of the hydrothermal system, implying a potential increase of phreatic eruptions. The time-series of interferometric synthetic aperture radar data, associated with the unrest episodes occurring in 2014–2015 and 2018–2019, revealed spatiotemporal variations of inflation episodes, centered around Oana crater, the most active fumarole of Azuma volcano. The modeled best-fit geometry of the elongated pressure source for the local deformation has the same dip as the overlying corresponded with the topographic slope direction and angle around Oana crater, suggesting the existence of topography-correlated layered structures within the hydrothermal system. In contrast, the broader deformation associated with the 2014–2015 unrest was explained by the overpressure of a horizontal flat source at 500 m below sea level, showing similar depth of the top as the conductive low-resistivity or low-viscous body suggested by previous studies. The unrest episodes were thus interpreted as mainly resulting from the supply of magmatic fluids or the volumetric thermal expansion of the hydrothermal system, caused by heat transfer from greater depths. Our study aids in understanding the shallow structure of this volcanic system and contributes towards evaluating the potential for forthcoming eruptions in Azuma volcano.
A nomogram model for predicting microvascular invasion of hepatocellular carcinoma based on multi-sequence MRI radiomics score and clinical-pathology-image parameters
Authors: wang qinghua , yang hongan , lei xiong , liu meng , he laichang , tan yongming 
Published: May 2023

Abstract
Objective:
Established aradiomics machine learning model based on multimodal MRI and clinical data, and analyzed the preoperative prediction value of this model formicrovascular invasion (MVI) of hepatocellular carcinoma (HCC).
Method:
The preoperative liver MRI data and clinical information of 130 HCC patients with pathologically confirmed were retrospectively studied. These patients were divided into MVI-positive group (MVI+) and MVI-negative group (MVI-) based on postoperative pathology. After a series of dimensionality reduction analysis, six features were finally selected. Then, linear support vector machine (linear SVM), support vector machine with rbf kernel function (rbf-SVM), logistic regression (LR), Random forest (RF) and XGBoost (XGB) algorithms were used to establish the MVI prediction model for preoperative HCC patients. Then, rbf-SVM with the best predictive performance was selected to construct the radiomics score (R-score). Finally, we combined R-score and clinical-pathology-image independent predictors to establish a combined nomogram model and corresponding individual models. The predictive performance of individual models and combined nomogram was evaluated and compared by receiver operating characteristic curve (ROC).
Result:
Alpha-fetoprotein concentration, peritumor enhancement, maximum tumor diameter, smooth tumor margins, tumor growth pattern, presence of intratumor hemorrhage, and RVI were independent predictors of MVI. Compared with individual models, the final combined nomogram model (AUC: 0.968, 95%CI: 0.920-1.000) constructed by radiometry score (R-score) combined with clinicopathological parameters and apparent imaging features showed the optimal predictive performance.
Conclusion:
This multi-parameter combined nomogram model hada good performance in predicting MVIof HCC, and hadcertain auxiliary value for the formulation of surgical plan and evaluation of prognosis.