bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new competitive strategy to unveil the antibiotic-producing Actinobacteria
Authors: Hussain Aehtesham , Patwekar Umera , Majumder Anirban , Majeed Aasif , Shouche Yogesh S 
Published: February 2023

ABSTRACTThe bacterial phylum Actinobacteria encompasses microorganisms with incomparable metabolic versatility and deep resource of medicines. However, the recent decrease in the discovery rate of antibiotics warrants innovative strategies to harness actinobacterial resources for lead discovery. Indeed, microbial culturing efforts measuring the outcomes of specific genera lagged behind the detected microbial potential. Herein, we used a distinct competitive strategy that exploits competitive microbial interactions to accelerate the diversification of strain libraries producing antibiotics. This directed-evolution-based strategy shifted the diversity of Actinobacteria over the experimental time course (0-8 days) and led to the isolation of Actinobacterial strains with distinct antimicrobial spectrum against pathogens. To understand the competitive interactions over experimental time, the metagenomic community sequencing revealed that actinobacterial members from familiesNocardiaceaeandCellulomonadaceaewith relatively increased abundances towards end, are thus competitively advantageous. Whilst comparing the Actinobacteria retrieved in the competitive strategy to that of the routinely used isolation method, the Actinobacteria genera identified from competitive communities differed relatively in abundances as well as antimicrobial spectrum compared to actinobacterial strains retrieved in classical method. In sum, we present a strategy that influences microbial interactions to accelerate the likelihood of potential actinobacterial strains with antimicrobial potencies.
Towards estimating marine wildlife abundance using aerial surveys and deep learning with hierarchical classifications subject to error
Authors: Augustine Ben C. , Koneff Mark D. , Pickens Bradley A. , Royle J. Andrew 
Published: February 2023

Aerial count surveys of wildlife populations are a prominent monitoring method for many wildlife species. Traditionally, these surveys utilize human observers to detect, count, and classify observations to species. However, given recent technological advances, many research groups are exploring the combined use of remote sensing and deep learning methods to replace human observers in order to improve data quality and reproducibility, reduce disturbance to wildlife, and increase aircrew safety. Given that deep learning detection and classification are not perfect and that statistical inference from ecological models is generally very sensitive to misclassification, we require study designs and statistical models to accommodate these observation errors.As part of an ongoing effort by the U.S. Fish and Wildlife Service, Bureau of Ocean Energy Management, and U.S. Geological Survey to survey marine birds and other marine wildlife using digital aerial imagery and deep learning object detection and classification, we developed a general hierarchical model for estimating species-specific abundance that accommodates object-level errors in classification. We consider hierarchical deep learning classification at multiple taxonomic levels subject to misclassification, hierarchically-structured human validation data subject to partial and erroneous misclassification, and an image censoring process leading to preferential sampling. We demonstrate that this model can estimate species-specific abundance and habitat relationships without bias when the assumptions are met, and we discuss the plausibility of these assumptions in practice for this study and others like it.Finally, we use this model to demonstrate the relevance of the features of the ecological systems under study to the classification task itself. In models that couple the ecological and classification processes into a single, hierarchical model, the true classes are treated as latent variables to be estimated and are informed by both the classification probability parameters and the ecological parameters that determine the expected frequencies of each class at the level the data are being modeled (e.g., site or site by occasion). We show that ignoring the expected frequencies of each class (when they are imbalanced) can cause correction for misclassification to produce biased parameter estimates, but coupling the ecological and classification models allows for the variability in relative class frequency across space and time due to ecological and sampling conditions to be accommodated with spatial or temporal covariates. As a result, bias is removed, classification is more accurate, and uncertainty is propagated between the ecological and classification models. We therefore argue that ability of deep learning classifiers, and classifiers more generally, to produce reliable ecological inference depends, in part, on the ecological system under study.
Reducing environmentally mediated transmission to moderate impacts of an emerging wildlife disease
Authors: Hoyt Joseph R. , Parise Katy L. , DePue John E. , Kaarakka Heather M. , Redell Jennifer A. , Scullon William H. , O'Reskie Rich , Foster Jeffrey T. , Kilpatrick A. Marm , Langwig Kate E. , White J. Paul 
Published: February 2023

Abstract

Emerging infectious diseases have caused population declines and biodiversity loss. The ability of pathogens to survive in the environment, independent of their host, can exacerbate disease impacts and increase the likelihood of species extinction. Control of pathogens with environmental stages remains a significant challenge for conservation and effective management strategies are urgently needed.

We examined the effectiveness of managing environmental exposure to reduce the impacts of an emerging infectious disease of bats, white‐nose syndrome (WNS). We used a chemical disinfectant, chlorine dioxide (ClO2), to experimentally reduce Pseudogymnoascus destructans, the fungal pathogen causing WNS, in the environment. We combined laboratory experiments with 3 years of field trials at four abandoned mines to determine whether ClO2 could effectively remove P. destructans from the environment, reduce host infection and limit population impacts.

ClO2 was effective at killing P. destructans in vitro across multiple concentrations. In field settings, higher concentrations of ClO2 treatment were needed to sufficiently reduce viable P. destructans conidia in the environment.

The reduction in the environmental reservoir at treatment sites resulted in lower fungal loads on bats compared to untreated control populations. Survival following treatment was also higher in little brown bats (Myotis lucifugus), and trended higher for tricolored bats (Perimyotis subflavus).

Synthesis and applications. Our results highlight that targeted management of sources for environmental transmission can be an effective control strategy for wildlife disease. We found that successfully reducing pathogen in the environment decreased disease severity and increased survival, but required higher treatment exposure than was effective in laboratory experiments, and the effects varied among species. More broadly, our findings have implications for other emerging wildlife diseases with free‐living pathogen stages by highlighting how the degree of environmental contamination can have cascading impacts on hosts, presenting an opportunity for intervention.
Group versus individual occupational therapy for toddlers with autism as a means to improve access to public health‐care services. Randomised controlled pilot study
Authors: Hirschmann Sivan , Magnezi Racheli , Bassan Haim , Tal Orna 
Published: February 2023

AbstractIntroductionIn recent years, the increasing prevalence of autism‐spectrum disorder has resulted in an increased demand for therapies including occupational therapy. In this pilot trial, we aimed to compare the efficacy of group versus individual occupational therapy among toddlers with autism as a means to improve accessibility to care.MethodsToddlers (2–4 years) undergoing autism evaluation in our public child developmental centre were recruited and randomised to receive 12 weekly sessions of group or individual occupational therapy based on the same mode of intervention: Developmental, Individual‐Differences and Relationship‐based (DIR). Primary outcomes related to intervention implementation included waiting days, nonattendance, intervention period, number of sessions attended and therapist satisfaction. Secondary outcomes were the Adaptive Behaviour Assessment System questionnaire, the Paediatric Quality of Life Inventory and the Peabody Developmental Motor Scale (PDMS‐2).ResultsTwenty toddlers with autism were included, 10 in each occupational therapy mode of intervention. Children waited fewer days before beginning group occupational therapy compared to individual therapy (52.4 ± 28.1 vs. 108.8 ± 48.0 days p < 0.01). Mean numbers of nonattendance was similar for both interventions (3.2 ± 2.82 vs. 2 ± 1.76, p > 0.05). Worker satisfaction scores were similar at the beginning and end of the study (6.1 ± 0.4 vs. 6.07 ± 0.49, p > 0.05). There were no significant differences between the percentage changes in individual and group therapy outcomes for adaptive score (6.0 ± 16.0 vs. 4.5 ± 17.9, p > 0.05), quality of life (1.3 ± 20.9 vs. 18.8 ± 24.5, p > 0.05) and fine motor skills (13.7 ± 36.1 vs. 15.1 ± 41.5, p > 0.05).ConclusionsIn this pilot study, the group DIR‐based occupational therapy for toddlers with autism improved access to services and allowed earlier interventions, with no clinical inferiority to individual therapy. Further research is required to examine group clinical therapy benefit.
Hsa_circ_0000591 drives osteosarcoma glycolysis and progression by sequestering miR‐194‐5p and elevating HK2 expression
Authors: Wang Dawei , Wang Yang , Wang Huadong , Yang Yafeng , Li Li , Liu Yihao , Yin Xin 
Published: February 2023

AbstractOsteosarcoma (OS) is the most common bone tumour with a high risk of metastatic progression and recurrence after treatment. Circular RNA hsa_circ_0000591 (circ_0000591) plays a compelling role in OS aggressiveness. However, the function and regulatory mechanism of circ_0000591 need to be further elucidated. As a subject of this study, a differential circRNA circ_0000591 was screened by circRNA microarray expression profiling (GSE96964). Expression changes of circ_0000591 were detected using real‐time quantitative polymerase chain reaction (RT‐qPCR). Effects of circ_0000591 silencing on OS cell viability, proliferation, colony formation, apoptosis, invasion, and glycolysis were determined via functional experiments. The mechanism by which circ_0000591 functions as a molecular sponge for miRNAs was predicted using bioinformatics analysis and validated using dual‐luciferase reporter and RNA pull‐down assays. Xenograft assay was done to validate the function of circ_0000591. Circ_0000591 was strongly expressed in OS samples and cells. Silencing of circ_0000591 lessened cell viability, repressed cell proliferation, invasion, glycolysis, and promoted cell apoptosis. Importantly, circ_0000591 regulated HK2 expression by serving as a miR‐194‐5p molecular sponge. MiR‐194‐5p silencing impaired circ_0000591 downregulation‐mediated suppression of OS cell malignancy and glycolysis. HK2 overexpression weakened the inhibiting impacts of miR‐194‐5p on OS cell malignancy and glycolysis. Also, circ_0000591 silencing decreased xenograft tumour growth in vivo. Circ_0000591 drove OS glycolysis and growth by upregulating HK2 by sequestering miR‐194‐5p. The study highlighted the tumour‐promoting function of circ_0000591 in OS.
Diversity, evolution and life strategies of CbK‐like phages
Authors: Ma Ruijie , Chen Xiaowei , Li Yingying , Jiao Nianzhi , Zhang Rui 
Published: February 2023

AbstractCaulobacter phage CbK has been extensively studied as a model system in virology and bacteriology. Lysogeny‐related genes have been found in each CbK‐like isolate, suggesting a life strategy of both lytic and lysogenic cycles. However, whether CbK‐related phages can enter lysogeny is still undetermined. This study identified new CbK‐like sequences and expanded the collection of CbK‐related phages. A common ancestry with a temperate lifestyle was predicted for the group, however, which subsequently evolved into two clades of different genome sizes and host associations. Through the examination of phage recombinase genes, alignment of attachment sites on the phage and bacterial genomes (attP–attB pairing), and the experimental validation, different lifestyles were found among the different members. A majority of clade II members retain a lysogenic lifestyle, whereas all clade I members have evolved into an obligate lytic lifestyle via a loss of the gene encoding Cre‐like recombinase and the coupled attP fragment. We postulated that the loss of lysogeny may be a by‐product of the increase in phage genome size, and vice versa. Clade I is likely to overcome the costs through maintaining more auxiliary metabolic genes (AMGs), particularly for those involved in protein metabolism, to strengthen host takeover and further benefit virion production.
Validation of a new method of providing case‐specific time‐of‐death estimates using cadaver temperatures
Authors: Abraham John , Wei Tie , Cheng Lijing 
Published: February 2023

AbstractThe near ubiquitous presence of numerical simulation has made case‐specific calculations of body temperatures following death possible so that accurate calculations of body temperatures can provide valuable information for estimating the time of death and can aid in forensic investigations. Here, a computational approach is described that has been validated against multiple, independent, and experimental investigations. The approach only requires one subjective input parameter (the heat transfer coefficient). A simple algorithm serves as a guidepost to the selection of this parameter. The algorithm incorporates clothing and the space in which the body is housed. Heat transfer coefficients that range from h = 2 W/m2/°C for bodies that are heavily clothed to h = 9 W/m2/°C for bodies that are nude (in air). The method also requires setting of ambient temperature conditions (ambient temperature)—however, that input is often available. The paucity of inputs makes this technique remarkably easy to employ. The new method is also able to calculate cadaver cooling rates for situations where the cadaver is in a timewise or spatially changing thermal environment (diurnal temperature variations, bodies partially submerged in water, changes to weather, insolation, etc.). Results from the present calculations are compared with a large body of measurements from the literature; it was found that the predictions and measurements were in excellent agreement, regardless of the ambient temperature conditions and the nature of the clothing of the body. This new calculation approach can be used with reasonable accuracy for determining cadaver cooling and time since death.
Formation and Evolution of Chromitites in the Hongshishan Complex, Beishan Orogenic Collage, NW China: Constraints from Mineralogical Compositions, Re‐Os Isotopes and Platinum‐Group Element Geochemistry
Authors: WANG Zhaolin , YAN Jiayong , ZHENG Xiaoming , TANG Hejun , YUAN Lulu , DENG Zhen , FANG Tonghui 
Published: February 2023

AbstractThe Hongshishan chromitite deposits are situated to the north of the Beishan orogenic collage, in the southern part of the Central Asian Orogenic Belt. This study describes the mineral chemistry, Re‐Os isotopes and platinum‐group elements geochemistry of the Hongshishan chromitites for the purpose of constraining the origin, evolution and composition of their parental melts. The restricted ranges of Al2O3, Cr2O3 and Cr#‐Mg# variation of chromite‐cores and chromites fall within the field of the mid‐ocean ridge and ophiolitic podiform chromite settings. The (187Os/188Os)i ratios of the chromitites are in the range of 0.12449–0.12745 (average 0.12637) and the γOs are from –1.92 to –0.06 (average –0.83). In the Re‐Os isotope diagrams, all the samples fall in the field of chromitites and show a residual peridotitic trend. The range of Os isotopic compositions and γOs values indicate that they overlap the depleted MORB mantle (DMM) as well as being close to global Os isotopic data and γOs of ophiolite chromitites. The characteristics of the PGE contents can be roughly subdivided into two groups: podiform chromitites and Ural‐Alaskan type complexes. For the ferritchromite cores, the calculated Al2O3 concentrations of the parental melt are higher (average 16.65 wt%) in high‐Cr than high‐Al chromitite (average 16.17 wt%) and for the chromite, the calculated Al2O3 concentrations are even higher (average 16.48 wt%) in the high‐Cr than the high‐Al examples (average 15.38 wt%). In the (TiO2)melt vs. TiO2 diagrams, most high‐Al melts fall in the MORB, while the high‐Cr melts fall in the ARC field. The calculated FeO/MgO ratios for the parental melt show the closest resemblance to a MORB magma composition. The inferred parental melt composition for studied chromitites falls in the field of mid‐ocean ridge basalt (MORB) magmas and far away from boninite. The calculated degrees of partial melting producing the chromitites are 16%–22% (average 19%), which is around the range of those of the MORB magmas. The chromitites are suggested to have been formed in a MORB setting. The chromites and ferritchromite cores are mostly scattered along the MORB and SSZ harzburgite–dunite fields. Ferritchromite rims and ferritchromites with high YFes formed as a result of alteration during serpentinization. .
Ross procedure after prosthetic valve thrombosis in a patient with antiphospholipid syndrome and recurrent bleeding
Authors: Mirzai Saeid , Badwan Osamah Z. , Sankar Parvathy R. , Karmali Rehan , Almaaitah Saja , Gomes Marcelo P. , Miyasaka Rhonda , Quatromoni Neha , Tong Michael Zhen‐Yu , Wassif Heba 
Published: February 2023

AbstractThe Ross procedure allows replacement of a diseased aortic valve with pulmonary root autograft, possibly avoiding the highly thrombotic mechanical valves and immunologic deterioration of tissue valves in antiphospholipid syndrome (APS). Here, we present the use of the Ross procedure in a 42‐year‐old woman with mild intellectual disability, APS, and a complex anticoagulation history after she presented with thrombosis of her mechanical On‐X aortic valve previously implanted for non‐bacterial thrombotic endocarditis.
Debunking the myth of the “robust control regime”: UK arms export controls during war and armed conflict
Authors: Stavrianakis Anna 
Published: February 2023

AbstractThe UK's commitments to conflict prevention and the protection of human rights and international humanitarian law in its arms export controls are now over 20 years old. However, the outbreak of war or conflict has had little or no restraining effect on UK arms exports. This article explores the function of the UK's arms export control regime given that its primary effect is not to restrict arms transfers. I argue that the mantra that the UK has one of the most robust control regimes in the world is not a plausible description of the realities of UK export policy – rather, it is a myth that needs to be debunked. Export controls are primarily mobilised by the state to manage controversy once criticism emerges from civil society and Parliament and thus primarily serve a legitimating function. I illustrate this argument with examples of arms exports in relation to the conflicts in Kashmir, Sri Lanka, the Occupied Palestinian Territory and Yemen, demonstrating three ways in which UK export controls are missing in action: the routine misuse of UK‐supplied weapons; the narrow interpretation of risk in licensing policy; and self‐serving reviews to manage reputation once controversy breaks out.
Unpacking the storytelling around French arms sales: Demystifying the “strategic autonomy” argument
Authors: Soubrier Emma 
Published: February 2023

AbstractThis article looks at the way France's arms exports have historically been framed as an inherent part of the country's core policies to achieve “strategic autonomy” at the domestic and international levels. It posits that taking this argument for granted without subjecting it to critical scrutiny is hazardous because it enables the continued unquestioned support for French arms sales and hampers the development of more stringent export control processes. Today, the need to look beyond this blanket storytelling is heightened in a context where unchecked exports relying on outdated views of global dynamics have precisely started to undermine France's strategic autonomy and interests. Not only does the country's growing dependence on exports create an antinomy of arms sales as a means of strategic independence, but the phenomenon of clients' reverse influence onto suppliers amidst a consolidated buyer's market also calls into question the autonomy of France's foreign policy.
Managers' private communications with analysts: The effect of SEC v. Siebel Systems, Inc.
Authors: Ali Ashiq , Durney Michael T. , Fisch Jill , Kyung Hoyoun 
Published: February 2023

AbstractIn 2005, the SEC suffered a high‐profile loss in its first court case, SEC v. Siebel Systems, Inc., in an effort to enforce Regulation Fair Disclosure (Reg FD). We examine the impact of this loss on managers' selective disclosure to sell‐side analysts. We provide evidence that the informativeness of analyst reports increased after the Siebel decision, especially for observable instances of private meetings. This finding suggests that such selective disclosure increased significantly after the court's decision. Our results also suggest that the increased selective disclosure faded as the SEC resumed enforcement actions related to Reg FD in 2009. In exploratory analyses, we survey and interview law firm partners to investigate possible mechanisms for our results; their responses suggest that the Siebel outcome reduced manager concern about liability from selective disclosure. Collectively, our results highlight how the anticipated costs of regulatory enforcement affect private information flow from managers to analysts in particular.