bluuebunny/crossref_metadata_2025_split huggingface dataset:
TMT-based quantitative proteomics reveals that electroacupuncture has neuroprotective effect on Alzheimer's disease rats through synaptic vesicle cycle and glutamatergic synapse signal pathways
Authors: Ke Chao , Shan Shengtao , Tan Yan , Cao Yang , Xie zhengrong , Pan Jiang , Zhang Wei 
Published: May 2023

Abstract
Background༚Recently, there has been increasing evidence that acupuncture as a kind of Traditional Chinese therapeutic method is significantly effective and safe way to treat Alzheimer’s disease (AD). This study provides supporting evidence and mechanism explanation of the therapeutic benefits of EA in the treatment of AD.
Method, In this study, we used tandem mass tagged (TMT) quantitative proteomics and bioinformatics analysis to seek core differentially expressed proteins (DEPs) in the typical pathways. And the DEPs were validated by parallel reaction monitoring (PRM).
Results, weidentified 211 proteins thatwere differentiallyexpressed among the different groups. Finally, we identified the following key DEPs:A0A0G2QC31(protein tyrosine phosphatase, PTP) and P31647 (Sodium- and chloride-dependent GABA transporter 3, GAT3), which are enriched in the synaptic vesicle pathway and glutamatergic synaptic pathway respectively.Validation of protein expression by PRM, it was found that the downregulation of GAT3 and PTP in the hippocampus of AD rats after EA treatment. These key proteins and related signaling pathways might be the possible mechanisms of AD treatment.
Conclusion, EA may be providing a neuroprotective effect in cognitive disorders through glutamate synaptic signaling pathway and synaptic vesicle signalingpathway in Aβ1–42-induced AD rat model.
Motivation moderates gender differences in navigation performance
Authors: Schinazi Victor R. , Meloni Dario , Grübel Jascha , Angus Douglas J. , Baumann Oliver , Weibel Raphael P. , Jeszenszky Péter , Hölscher Christoph , Thrash Tyler 
Published: May 2023

Abstract

Gender differences in navigation performance are a recurrent and controversial topic. Previous research suggests that men outperform women in navigation tasks and that men and women exhibit different navigation strategies. Here, we investigate whether motivation to complete the task moderates the relationship between navigation performance and gender. Participants learned the locations of landmarks in a novel virtual city. During learning, participants could trigger a top-down map that depicted their current position and the locations of the landmarks. During testing, participants were divided into control and treatment groups and were not allowed to consult the map. All participants were given 16 minutes to navigate to the landmarks, but those in the treatment group were monetarily penalized for every second they spent completing the task. Results revealed a negative relationship between physiological arousal and the time required to locate the landmarks. In addition, gender differences in strategy were found during learning, with women spending more time with the map and taking 40% longer than men to locate the landmarks. Interestingly, an interaction between gender and treatment group revealed that women in the control group required more time than men and women in the treatment group to retrieve the landmarks. During testing, women in the control group also took more circuitous routes compared to men in the control group and women in the treatment group. These results suggest that a concurrent and relevant stressor can motivate women to perform similarly to men, helping to diminish pervasive gender differences found in the navigation literature.
WDR72 as a Novel Prognosis-Predictive Biomarker Linked to Immune Infiltration and Poor Prognosis in Clear Cell Renal Cell Carcinoma
Authors: liu Jinhui , Xiao Lei , Luo Sheng , Liu Jiayuan , Tang Yuanjia , Zhou Benzheng 
Published: May 2023

Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is a highly heterogeneous cancer type that lacks early diagnostic markers and effective targets for immunotherapy. WD repeat-containing protein 72 (WDR72) mutations have been linked to renal dysfunction and renal tubular acidosis. However, the potential prognosis-predictive value of WDR72 in ccRCC remains unelucidated.
Methods: The current research used The Cancer Genome Atlas (TCGA) data to explore the expression and prognosis-predictive value of WDR72 in various solid tumors, including ccRCC. The analysis was validated using the TCGA–ccRCC cohort and Human Protein Atlas. The biological processes and signaling pathways linked to the expression of WDR72 were identified by conducting Gene Ontology and gene set enrichment analysis. Cox regression analysis was utilized to measure the prognosis-predictive value of WDR72 in ccRCC. Moreover, the association of WDR72 expression levels with immune cell infiltration, methylation, m6A, tumor mutation burden, immune checkpoint molecules, and drug sensitivity was analyzed. Finally, the study was verified by conducting quantitative real-time polymerase chain reaction and cytological experiments in ccRCC.
Results: A pan-cancer analysis noted that low WDR72 expression levels were strongly linked to poor overall survival and progression-free interval in individuals with ccRCC. The WDR72 levels were remarkably reduced in ccRCC tissues in comparison with the adjacent tissues, and WDR72 was identified as an independent predictor of patients with ccRCC. Pathway enrichment analysis showed a close relationship between genes related to WDR72 and fat metabolism. Low WDR72 expression in ccRCC was linked to higher immune cell infiltration, stronger tumor mutation burden, and higher immune checkpoint molecules. Furthermore, individuals with ccRCC and low WDR72 expression had lower IC50 values for most drugs.
Conclusion: Low WDR72 expression is strongly linked to the progression, prognosis, and microenvironmental immune regulation of individuals with ccRCC. Moreover, it may hold potential as a valuable prognostic biological marker and therapeutic measure for ccRCC. The findings provide a basis for future research by exploring the role of WDR72 in ccRCC tumor development.
Parent-Child Interaction as an Effective Tool for Identifying Autism Spectrum Disorder: Evidence from EEG Analysis
Authors: Deng Lin , He Wei-zhong , Wei Ling , Dai Yuan , Liu Yu-qi , Chen Zi-lin , Gong Jing-bo , Li Fei 
Published: May 2023

Abstract
Background
Autism Spectrum Disorder (ASD) is a complex neurodevelopmental disorder that affects individuals across their lifespan. Early diagnosis and intervention are crucial for improving outcomes. However, current diagnostic methods are often time-consuming, subjective, and costly, making them inaccessible to many families. In this study, we aimed to investigate the use of parent-child interaction as a potential screening tool for identifying children with ASD.
Methods
We recruited 85 preschool ASD children with development delay (DD), ASD children without DD and typically developing (TD) children, along with their caregivers, to engage in parent-child interaction while simultaneously recording EEG and video signals.
Results
We found that the behavioral indicators of both ASD groups were worse than TD group and correlated with the severity of the symptoms of ASD. Both theta and alpha band PSD values increased in both ASD groups compared to TD children, primarily correlated with social behavior rather than IQ level.
Conclusion
Our results verified the behavioral paradigm of parent-child interaction as an efficient method for identifying children with ASD, further validated by the PSD of alpha and theta bands.
Synthesis of Novel Coumarin Derivatives from N,N-dimethylbarbituric Acid and Dimedone via Domino Knöevenagel Hetero-Diels–Alder Reactions
Authors: Asadi Mina , Zarezadeh Nahid , Bahadorikhalili Saeed , Mahdavi Mohammad 
Published: May 2023

Abstract
In this paper, the successful synthesis of several novel coumarin derivatives is reported. For this synthesis, (E)-N-(3-formyl-2-oxo-2H-chromen-4-yl)-N-methyl-2-phenylethene-1-sulfonamide was first prepared in three steps, from the conversion reaction of styrene to (E)-2-phenylethene-1-sulfonyl chloride, followed by 4-chloro-2-oxo-2H-chromene-3-carbaldehyde. The product is reacted with N,N-dimethylbarbituric acid, and dimedone in water. Whereas N,N-dimethylbarbituric underwent traditional domino Knöevenagel hetero-Diels–Alder reaction, affording pyranofused-δ-sultam-annulated coumarins, chromenocoumarin derivatives were obtained using dimedone through a novel type of domino Knöevenagel hetero-Diels–Alder reaction.
Risk-Benefits Assessment of Calcium Supplementation Based on Multi-Dimensional Nutritional Profiles in a Cross-sectional Study
Authors: Mirani Sara , Horváth László , Vecsernyés Miklós , Tóth Béla E. 
Published: May 2023

Abstract
Background
Calcium intake is an essential component of a healthy diet and has been linked to numerous health benefits, including the prevention of osteoporosis and reduction of fracture risk in children and adults. The most plausible consequence of insufficient calcium intake can lead to osteoporosis over decades, which is a significant cause of morbidity and mortality with high burden of disease. However, attention should be given to the risk of potential overdoses, since some trials have linked excessive calcium supplementation to increased risk of health complications.
Methods
Our study aimed to evaluate the possible factors influencing the participants’ calcium profile through a “dietary intake specific questionnaire” to achieve the optimal calcium and vitamin D intake in the prevention of or during therapies for osteoporosis. A comprehensive multi-dimensional dietary survey was administered to evaluate various aspects of the participants' diets, including dietary and supplemented calcium intake, vitamin D exposure, vitamin supplementation profile, and relevant lifestyle parameters. In this article, we specifically analyzed the calcium intake profiles and pharmacovigilance aspects of the study participants.
Results
We received 478 evaluable datasets. The dietary calcium intake, on average, was close to optimal in the general public. Supplementation safety concerns were raised for the public in less than 7% of patients, who were reported to have a potential overload in daily calcium intake. Albeit, one-third (34%) of the population had a significant deficiency (average: 544 mg/day), and this effect was more relevant among ≤ 25-years old individuals, of them only less than 37% of the responders had an optimal calcium intake. More than half (52%) of the dietary calcium deficient participants did not take any additional calcium supplements. Although, in 73% of general population, most calcium supplementation dosage in use was equal to or below 200 mg/day.
Conclusion
Our study highlights the importance to implement a “Patient Focused Motivation Strategy” as a broader approach in pharmaceutical care for increasing the patient’s knowledge, understanding the needs of supplementation, and selecting the correct form and dose of the available non-prescription products. It is important to maintain motivation and adherence to the recommended therapy to achieve optimal health outcomes.
Impact of Interplay Effects on Spot Scanning Proton Therapy with Motion Mitigation Techniques for Lung Cancer: SFUD versus Robustly Optimized IMPT Plans Utilizing a Four-dimensional Dynamic Dose Simulation Tool
Authors: Yamano Akihiro , Inoue Tatsuya , Yagihashi Takayuki , Yamanaka Masashi , Matsumoto Kazuki , Shimo Takahiro , Shirata Ryosuke , Nitta Kazunori , Nagata Hironori , Shiraishi Sachika , Minagawa Yumiko , Omura Motoko , Tokuuye Koichi , Chang Weishan 
Published: May 2023

Abstract

Background: The interaction between breathing motion and scanning beam causes interplay effects in spot-scanning proton therapy for lung cancer, resulting in compromised treatment quality. Therefore, in this study, we investigated the impact of the effects and clinical robustness of two types of spot-scanning proton therapy with motion mitigation techniques for locally advanced non-small cell lung cancer (NSCLC) by utilizing a new simulation tool (4DCT calculation).
Methods: Three-field single-field uniform dose (SFUD) and robustly optimized intensity-modulated proton therapy (IMPT) plans, combined with gating and rescanning techniques, were created using a VQA treatment planning system for 15 patients with locally advanced NSCLC (70 GyRBE/35 fractions). In addition, gating windows of three or five phases around the end-of-expiration phase and two internal gross tumor volumes (iGTVs) were created, and a rescanning number of four was used. At first, the static dose (SD) was calculated using end-of-expiration computed tomography (CT). Then, the four-dimensional dynamic dose (4DDD) was calculated using the SD plans, 4D-CT images, and deformable image registration technique on the end-of-expiration CT. The target coverage (V98%, V100%), homogeneity index, and conformation number for the iGTVs and organ-at-risk (OAR) doses were calculated for the SD and 4DDD groups and compared between both treatment plans.
Results: In the 3- and 5-phase SFUD, statistically significant differences between SD and 4DDD were observed for V100%, homogeneity, and conformity. In contrast, statistically significant differences were observed for V98%, V100%, and homogeneity in both the 3- and 5- phases of IMPT. The mean V98% and V100% in both 3-phase plans were within the clinical limits (>95%) when the interplay effects were considered; however, V100% decreased to 89.3% and 94.0% for the 5-phase SFUD and IMPT, respectively.
Conclusions: The interplay effects had a limited impact on target coverage and OAR doses in SFUD and robustly optimized IMPT with 3-phase gating and rescanning for patients with locally advanced NSCLC. Furthermore, the target coverage deteriorated considerably as the gating window increased. This study demonstrated that robustly optimized IMPT is more robust than SFUD to the interplay effect in terms of target coverage with the prescription dose and homogeneity.
Trial registration: None.
A comparative analysis of genetic differentiation between two sympatric and closely related species, D. bipectinata and D. malerkotliana
Authors: Singh Gurvachan , Singh Arvind Kumar 
Published: May 2023

Abstract
D. bipectinata and D. malerkotliana are two phylogenetically closely related species that share common ecological niches throughout their distribution zone which comes under Oriental-Australian zoogeographical regions. These two species have been found to share several common genetic characteristics and due to this, they may experience interspecific mating under laboratory conditions and produce hybrid progeny with sterile males and less fertile females. The population genetical work on these two species has been inadequately done by considering cosmopolitan inversions and enzyme polymorphisms. We decided to consider the genetic polymorphism involving commonly persistent chromosomal inversions, allozymes and microsatellite variants of the two species to envisage genetic differentiation among the natural populations of these two species sampled from distant localities of Indian cities. The results of this study indicate that Indian populations of both the species are genetically structured. There exists graded variation (clinal variation) in the level of heterozygosity from north to south as an increase in the observed heterozygosity prevailed from north to south. This trend was observed in the populations of both the species that hints towards similar genetic changes being experienced by its members all along their distribution area. The phylogenetic trees based on the extent of genetic identity between the paired populations of these two species portray two distinct clusters, one for the two populations of north and the other for the remaining populations of south. Further, through this study, it can be stated with certainty that there exists ‘isolation by distance’ as the north and south populations of both the species genetically significantly vary from each other.
ECG Signal Quality in Intermittent Long-Term Dry Electrode Recordings with Controlled Motion Artifacts
Authors: Joutsen Atte , Cömert Alper , Kaappa Emma , Vanhatalo Kirsi , Riistama Jarno , Vehkaoja Antti , Eskola Hannu 
Published: May 2023

Abstract
Wearable long-term monitoring applications are becoming more and more popular in both the consumer and the medical market. In wearable ECG monitoring, the data quality depends on the properties of the electrodes and on how they contact the skin. Dry electrodes do not require any action from the user. They usually do not irritate the skin, and they provide sufficiently high-quality data for ECG monitoring purposes during low-intensity user activity. We investigated prospective motion artifact–resistant dry electrode materials for wearable ECG monitoring. The tested materials were 1) porous: a conductive polymer, conductive silver fabric; and 2) solid: stainless steel, silver, and platinum. ECG was acquired from test subjects in a 10-minute continuous settling test and in a 48-hour intermittent long-term test. In the settling test, the electrodes were stationary, whereas both stationary and controlled motion artifact tests were included in the long-term test. The signal-to-noise ratio (SNR) was used as a figure of merit to quantify the results. The SNR of all electrode types increased during the settling test. In the long-term test, the SNR was generally elevated further. The introduction of electrode movement reduced the SNR markedly. Solid electrodes had a higher SNR than porous electrodes. In the stationary testing, stainless steel showed the highest SNR, followed by platinum, silver, conductive polymer, and conductive fabric. In the movement testing, the order was platinum, stainless steel, silver, conductive polymer, and conductive fabric.
Efficacy and mechanism of a biomimetic nanosystem carrying doxorubicin and an IDO inhibitor for treatment of advanced triple-negative breast cancer
Authors: Hu Chuling , Liu Yan , Cao Wei , Li Na , Gao Shen , Wang Zhuo , Gu Fenfen 
Published: May 2023

Abstract
As a kind of “cold tumor”, triple-negative breast cancer has a bottleneck in immunotherapy. In this study, mesoporous silica nanoparticles were coated with the chemotherapeutic drug doxorubicin and indoleamine 2, 3-dioxygenase 1 inhibitor 1-MT, and the outer layer was coated with a triple-negative breast cancer cell membrane to construct the tumor dual-targeted delivery system CDIMSN for chemotherapy and immunotherapy, and to investigate the immunogenic death effect of CDIMSN. The system targeted the delivery of tumor therapeutic drugs to the tumor microenvironment. Doxorubicin induced tumor immunogenic death, while 1-MT reversed immunosuppression. In vitro experiments showed that IC50 value of CDIMSN was 0.34µg/ml, significantly lower than that of DIMSN (0.56µg/ml). In vivo findings showed that the tumor size in the CDIMSN group was 2.66-fold and 1.56-fold smaller than that in DOX and DIMSN groups, respectively. CDIMSN group was better than naked DIMSN in stimulating CD8+T cells, CD4+T cells and promoting DCs cell maturation. In addition, blood analysis, biochemical analysis and Hematoxylin staining analysis of mice showed that the bionic nanoparticles had good biological safety.
Energy efficient cluster head using modified fuzzy logic with WOA and path selection using Enhanced CSO in IoT-enabled smart agriculture systems
Authors: C Senthil kumar , R Vijay Anand 
Published: May 2023

Abstract
The advent of Internet of Things (IoT) in agriculture has revolutionized the way farmers monitor and manage their crops. IoT-enabled sensors can provide real-time data on various environmental parameters such as temperature, humidity, soil moisture, and crop growth, which can be used to make informed decisions and optimize crop yield. However, the vast amount of data generated by these sensors poses a significant challenge in terms of data processing and communication. To address this challenge, clustering is often used to group the sensors into clusters and elect a Cluster Head (CH) to communicate with the gateway node. The selection of an appropriate CH and the optimal path for data transmission are critical factors that affect the performance of the IoT system. In this paper, we propose a novel approach to optimize the CH selection and path selection using modified Fuzzy Logic, Whale optimization algorithm (WOA) and Enhanced Crow Swarm Optimization (ECSO). Fuzzy Logic is used to evaluate the relevant parameters such as energy, distance, overhead, trust, and node degree to select the most suitable CH. ECSO is then employed to find the optimal path for data transmission based on the selected CH. We evaluate the proposed approach using simulation experiments in a smart agriculture scenario. The results show that our approach outperforms existing approaches in terms of throughput, packet delivery ratio, delay, and energy efficiency. Our proposed approach can significantly improve the performance of IoT-enabled smart agriculture systems, leading to better crop yield and higher profitability for farmers. The results of our simulation experiments demonstrate the superiority of our approach over existing one’s throughput, Packet Delivery Ratio (PDR), delay, energy consumption efficiency is found in the result section.
Proving Concurrency by Loci
Authors: Salehi Saeed 
Published: May 2023

Abstract
A fascinating and catchy method for proving that a number of special lines are concurrent is using the concept of locus. This is now the classical method for proving the concurrency of the internal angle bisectors and perpendicular side bisectors of a triangle. In this paper, we prove the concurrency of the altitudes and the medians by showing that they are loci of some interesting points. Our proofs for these ancient theorems seem to be new. We also provide loci method proofs for the concurrency theorems of Ceva and Carnot.
AMS 2020 MSC: 51M04.
Effects of temperature and salinity on tissue structure, antioxidant capacity and heat shock protein gene expression of Penaeus monodon
Authors: Yang Qibin , Yang Wanli , Jiang Song , Kong Dewei , Jiang Shigui , Huang Jianhua , Yang Lishi , Li Yundong , Zhou Falin 
Published: May 2023

Abstract
In order to explore the influence of temperature and salinity on stress response of P. monodon, four experimental groups were set, respectively were normal temperature and normal salt (Control group), normal temperature and low salt(Group A), high temperature and low salt(Group B), high temperature and normal salt(Group C). The stress experiment was conducted for 96 hours, and the activities of acid phosphatase(ACP), alkaline phosphatase(AKP), total superoxide dismutase (T-SOD) and total antioxidant capacity (T-AOC) in hepatopancreas, the changes of tissue structure and the expression of related heat shock protein genes were measured．The results showed that the branchial structures of the three stress groups were found to be damaged to varying degrees. In Group A, AKP and ACP levels increased initially and then decreased, while T-SOD levels decreased initially and then steadily increased. In Group B and Group C, the four enzymes showed similar trends.There was no significant difference in the expression level of Hsps in the low salt group. The expression levels of Hsp-10, Hsp-60 and Hsp-90 genes showed a similar trend, and the maximum expression levels of the four genes were all at 96h. The expression levels of Hsps in muscle tissue were similar to those in hepatopancreas tissue. However, the highest expression levels of Hsp-70 and Hsp-90 in high temperature and low salt group were at 24h. Through this work on P. monodon stress responses to high temperature and low salinity, we have gained a deeper understanding of the physiological responses to environmental stressors, which will be useful in the development of protective measures for the shrimps under aquaculture conditions that replicate these stressors.