bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilization of modified mRNA encoding bone morphogenetic protein-2 for periodontal regeneration: an in vitro study
Authors:   , Kulthanaamondhita Promphakkon 
Published: September 2023

Current modalities for periodontal regeneration provide modest success, however complete periodontal regeneration is still not achievable. Recently in vitro synthesized nucleoside-modified messenger RNA (mRNA) has emerged as a novel platform in regenerative medicine. The aims of this study are to investigate the ability of human periodontal ligament cells (PDLCs), clinically relevant target cells, to produce bone morphogenetic protein-2 (BMP-2), a significant protein for bone formation after transfected with modified mRNA that encode this protein. We investigated the ability of translated protein for enhancing PDLC proliferation and promotes endothelial cell tube formation, marker of angiogenesis. Isolated PDLCs from healthy periodontal tissue were transfected with N1-methylpseudouridine modified mRNA encoding BMP-2 complexed with transfecting agent, Lipofectamine 2000. Cell lysates and supernatants were collected at 24, 48 and 72 hours (h) after transfection for protein production by ELISA and cell viability by AlamarBlue assay.

High levels of BMP-2 production were detected intracellulary and extracellulary. Secreted BMP-2 gradually increased up to 72 h. Cell viability was maintained above 90% throughout the observation period. The post-transfection supernatants were able to promote PDLC proliferation and endothelial cell tube formation. In conclusion, transfection of PDLCs with N1-methylpseudouridine modified mRNA encoding BMP-2 in lipofectamine 2000 led to high levels of functional BMP-2 protein. Using the in vitro synthesized nucleoside-modified mRNA may allow future application as novel therapeutics platform for periodontal regeneration, however further researches are required.
The use of modified mRNA encoding platelet-derived growth factor-BB as an innovation in periodontal regeneration
Authors:   , Surisaeng Theeraphat 
Published: September 2023

Since the mRNA platform has been recently introduced to several fields in medicine and the achievement of periodontal regeneration is currently unpredictable, therefore, the use of mRNA technology is a promising approach to pursue the complete periodontal regeneration. The aims of this study are to learn if mRNA encoding platelet-derived growth factor-BB (PDGF-BB) induces PDGF production in human periodontal ligament cells (PDLCs) and to investigate the effect of secreted PDGF on PDLC proliferation and promoting endothelial cell tube formation. PDLCs were obtained from extracted teeth of healthy periodontal patients. The N1-methylpseudouridine modified mRNA encoding PDGF-BB were transfected into PDLCs. The supernatants were collected from 24-, 48- and 72 h and measured the protein production using ELISA assay. PDLCs were also tested for cell viability. In addition, the supernatants collected at 48 h and 72 h were used for proliferation assay and induction of endothelial cell tube formation, respectively. The results showed that PDLCs transfected with mRNA encoding PDGF-BB, produced higher level of intracellular PDGF-BB than the control at 24 h. PDGF-BB was detected in the supernatants started from 24 h and constantly secreted up to 72 h. The transfection of PDLCs by mRNA had no effect on the cell viability. The supernatants containing secreted PDGF-BB were able to promote PDLC proliferation and endothelial cell tube formation. In conclusion, modified mRNA encoding PDGF-BB promotes PDGF-BB production both intra- and extracellularly. Moreover, the supernatants containing PDGF-BB induce PDLC proliferation and endothelial cell tube formation. Thus, mRNA platform technology is possibly applicable for periodontal tissue regeneration.
Screening and identification of probiotic lactic acid bacteria from plant samplesd
Authors:   , Nuhwa Ratthanatda 
Published: September 2023

Seventy lactic acid bacteria (LAB) were isolated from flowers (17 strains), 3 tree barks (7 strains), one fruit (2 strains), ten fermented tea leaves (31 strains) and two silage (13 strains). They were identified as Lactobacillus pentosus (12 strains), L. plantarum subsp. plantarum (9 strains), L. paracasei subsp. tolerans (5 strains), L. brevis (1 strain), L. silagincola (1 strain), L. kunkeei (1 strain), and L. formosensis (1 strain), Enterococcus durans (3 strains), E. lactis (2 strains), E. faecalis (1 strain), E. faecium (1 strain), E. gallinarum (1 strain) and E. gilvus (1 strain), Pediococcus acidilactici (1 strain) and P. pentosaceus (1 strain), and Aerococcus urinaeequi (1 strain) based on their phenotypic characteristics and 16S rRNA gene sequences. Strain FM11-1T, a novel species isolated from the flower of Solanum torvum, was closely related to E. faecium NRIC 1145T (98.79 %), E. durans NBRC 100479T (98.72 %), E. lactis LMG 25958T (98.49 %), and E. ratti DSM 15687T (98.02 %) based on the 16S rRNA gene sequence, genome sequence, and phylogenetic tree. Therefore, it was proposed as Enterococcus solani sp. nov. The screening of bile salt hydrolase, cholesterol assimilation, antimicrobial activity, and cytotoxicity against Caco-2 cells revealed that 28 strains showed bile salt hydrolase activity while 19 strains exhibited cholesterol assimilation more than 45 %. Strain CRM44-2 showed the highest cholesterol assimilation of 85.5 %. Furthermore, the strains FM3-1, FM11-1, FM11-2, CM28-3, CM38-1, CRM41-1, CRM55-2, and NWM60-2 exhibited bile salt hydrolase activity and cholesterol assimilation more than 45 %. Strain CRM51-2 showed antimicrobial activity against Bacillus subtilis ATCC 6633. Strains CM28-3 and CRM42-1 showed cytotoxic effects against colorectal cancer cell lines (Caco-2 cells) with non-toxicity to Vero cells. All isolates showed no cytotoxic effects against Vero and HepG2 cells. For the probiotic properties, 31 strains showed pH 3.0 and 0.3 % of bile acid tolerance for 3 hours by decreasing 2 log cycles while 13 strains showed the adhesion ability. L. brevis strain CM38-1 isolated from fermented tea leaves, showed bile salt hydrolase activity, cholesterol assimilation of 62.5 % and adhesion ability of 6.0 + 1.0 % as the candidate probiotics with cholesterol-lowering. Moreover, L. plantarum subsp. plantarum strain CM28-3 isolated from fermented tea leaves, showed bile salt hydrolase activity, cholesterol assimilation of 46.5 %, cytotoxicity against Caco-2 cells of 64.0 + 0.1 %, and adhesion ability of 0.7 + 1.0 %. Therefore, it is suggested to be the candidate probiotics with higher cholesterol-lowering activity compared to L. rhamnosus GG as positive control.
Continuous flow selective hydrogenation of 5-hydroxymethylfurfural to 2.5-dimethylfuran using Pd-Cu/reduced graphene oxde catalysts
Authors:   , Mhadmhan Sareena 
Published: September 2023

2,5-Dimethylfuran (DMF) has been considered a promising biofuel, potentially derived from biomass. There have been various reports on DMF production from hydrogenation of 5-hydroxymethylfurfural (HMF). However, most reports employed high hydrogen pressure, long reaction times, and reactions under batch reactor. In this study, Pd-Cu bimetallic catalysts incorporated on reduced graphene oxide (RGO) were used for hydrogenation of HMF to DMF using 2-propanol as hydrogen donor under continuous ﬂow system. Synthesized catalysts were characterized by N2 physisorption, scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and temperature programmed reduction of hydrogen (H2-TPR) techniques. 10Cu1Pd/RGO exhibited 96% HMF conversion with 95% DMF yield under optimum reaction conditions with good stability with time on stream. XRD and XPS results pointed to the presence of a palladium−copper alloy, which could enhance both the activity and especially the stability in the conversion of HMF toward DMF. The effect of temperature, pressure, and feed ﬂow rate were also investigated on the catalytic performance. The stability of catalyst was tested for 8 h time on stream, where it was found that the catalyst provided 67% HMF conversion with 65% DMF yield.
Taxonomy, bioactivities, and secondary metabolites of endophytic actinomycetes isolated from medicinal plants
Authors:   , Klykleung Nattaporn 
Published: September 2023

Fifty endophytic actinomycetes isolated from six medicinal plants in Thailand were identified as Micromonospora (34 strains), Streptomyces (8 strains), Microbispora (3 strains), Nonomuraea (2 strains), Plantactinospora (2 strains), and Amycolatopsis (1 strain) based on phenotypic characteristics and 16S rRNA gene sequencing. Fifteen strains exhibited antimicrobial activity against Gram-positive bacteria. Strain CR1-09T, proposed as Microbispora cathalanthi sp. nov. was active against Staphylococcus aureus ATCC 6538 and Kocuria rhizophila ATCC 9341. Streptomyces sp. 3MP-10 and 3MP-14, novel species were active against S. aureus ATCC 6538, K. rhizophila ATCC 9341, and Bacillus subtilis ATCC 6633. Strains CR1-09T and PA1-07 showed cytotoxicity against HepG2 cells but not Vero cells based on MTT assay. Streptomyces sp. 3MP-10 and 3MP-14 produced ikarugamycin ([M+H]+ = 479.2912 m/z; UV 224, 326 nm) and fumaquinone ([M+H]+ = 303.1225 m/z; UV 220, 236, 304, 428 nm) while 3MP-14 produced a new structural compound, pudicin ([M+Na]+ = 600.2576 m/z; UV 204, 246, 344 nm). Nonomuraea sp. PA1-05 and PA1-10 produced dilactonmycin ([M+H]+ = 539.3223 m/z; UV 222, 242, 316 nm) and spithioneine A ([M+H]+ = 492.1833 m/z; UV 250, 274, 336 nm), respectively. Micromonospora sp. BL1-08 produced riboflavin ([M+H]+ = 377.1466 m/z; UV 222, 268, 370, 446 nm) based on liquid chromatography-mass spectrometry (LC/MS) with the dictionary of natural products. Moreover, all strains produced a siderophore as nocardamine ([M+H]+ = 565.1552 m/z; UV 214, 272, 336 nm). The secondary metabolite biosynthetic gene clusters (BGCs) identified in the draft genome of strain 3MP-14 were related to the biosynthesis of polyketides by polyketide synthase (PKS-I, II, and III) as 41.7 % of all putative BGCs and non-ribosomal peptides by non-ribosomal peptide synthetase (NRPS).
In vitro hepatic metabolism of curcumin diethyl disuccinate in different animal species
Authors:   , Jithavech Ponsiree 
Published: September 2023

Curcumin diethyl disuccinate (CDD) is an ester prodrug of the active compound, curcumin. CDD has been improved chemical stability and intestinal permeability more than curcumin. CDD demonstrated biological activity higher than curcumin. The aim of this study is to investigate a suitability of using liver S9 fraction (LS9) in drug metabolism profiling as function of animal sources of enzyme regarding possible translation of in vitro outcome to clinical study. The metabolites, in vitro kinetics and esterase enzyme involved in CDD hydrolysis by LS9 of human, monkey, dog and rat ware determined. The high-performance liquid chromatography conjugated with high-resolution mass spectrometry-based methodologies indicated that CDD was hydrolysed to curcumin through the monoethylsuccinyl curcumin. Ultra-high-performance liquid chromatography analysis was used to determined kinetic parameters, including degradation rate constants, half-lives and hepatic intrinsic clearances. The hydrolysis profile of CDD was similar in all test species and showed a two-step, consecutive, pseudo-first-order irreversible reaction. CDD was rapidly metabolized in LS9 of all test species and ranked as human > dog > monkey > rat. The predicted clearance of CDD in liver might be ranked as dog > monkey > rat > human. The identification of CDD hydrolyzing enzymes from HLS9, MLS9, DLS9 and RLS9 evaluated by the effect of specific esterase inhibitor. Carboxylesterase was identified as the major hepatic enzyme responsible for CDD hydrolysis in LS9 of four species, followed by butyrylcholinesterase. The in vitro hepatic metabolism outcomes would be of benefit to future in vivo studies and the clinical translation of the ester CDD prodrug.
Physicochemical, permeability, antioxidant and anti-inflammatory properties of oxyresveratrol ester prodrugs
Authors:   , Thaweesest Wuttinont 
Published: September 2023

Oxyresveratrol (OXY) is known to exert antioxidant and anti-inflammatory properties but has low oral bioavailability in rats and low permeability in the Caco-2 cell monolayer model. Three ester prodrugs with different acyl chain lengths (oxyresveratrol tetraacetate (OXY-TAc), oxyresveratrol tetrapropionate (OXY-TPr), and oxyresveratrol tetrabutyrate (OXY-TBu) were synthesized to improve the permeability of OXY. Transport of three OXY ester prodrugs across Caco-2 monolayers was higher than that of OXY, and OXY-TAc exhibited the highest permeation. The degradation of OXY-TAc in HCl buffer (pH 1.2), phosphate buffer (pH 6.8 and 7.4), simulated gastric and intestinal fluids, as well as human plasma at 37 °C was followed pseudo-first-order kinetics. Antioxidant and anti-inflammatory effects, as well as mechanisms of action in LPS-stimulated murine macrophage cells (RAW264.7) under oxidative stress and inflammation of bioavailable fraction of OXY-TAc (BF-OXY-TAc), were investigated and compared with the bioavailable fraction of OXY (BF-OXY). All three antioxidant assays (1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) assays) showed that BF-OXY-TAc exhibited higher antioxidant activities than that of BF-OXY. In the LPS-stimulated RAW264.7 macrophage cells, BF-OXY-TAc attenuated the LPS-induced reactive oxygen species (ROS), nitric oxide (NO), and interleukin-6 (IL-6) levels were 2.5, 2.5, and 1.6-fold higher than that of BF-OXY respectively. Western blot analysis revealed that BF-OXY-TAc decreased inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions were 1.2 and 1.4-fold higher than that of BF-OXY, respectively. The results suggest that OXY-TAc can overcome the limitations of OXY in terms of improving permeation, leading to better antioxidant and anti-inflammatory activities of OXY against LPS-stimulated oxidative stress and inflammation in RAW264.7 macrophage cells.
The effect of surface treatment on shear bond strength of resin-matrix ceramics and dual-cured resin cement
Authors:   , Bunchuansakul Nuttapong 
Published: September 2023

Objective:The purpose of this study was to evaluate the effect of Tetrahydrofuran (THF) on shear bond strength (SBS) between resin matrix ceramics (RMC) materials and resin cements.Methods:RMC materials(Enamic,Cerasmart,Shofu block HC) were cut into square piece of approximately 6x6x2 mm3 and randomly divided into 10 groups following the surface treatment:no treatment(C),adhesive
agent(Ad),THF1min(T1),silane/adhesive(Si/Ad),THF1min/adhesive(T1/Ad),THF1min/silane/adhesive(T1/Si/Ad),THF2mins/silane/adhesive(T2/Si/Ad),THF3mins/silane/adhesive(T3/Si/Ad),THF4mins/silane/adhesive(T4/Si/Ad),THF5mins/silane/adhesive(T5/Si/Ad).Specimens were cemented to composite resin rod with resin cement and kept them in water at 37oC for 24 hours.The SBS measurements were tested with universal testing machine.Failure modes were examined by stereomicroscope.The SBS values were analyzed with two-way ANOVA and Bonferroni’s post hoc tests (α = 0.05).Results: The highest mean SBS for all RMC materials was found in group T3/Si/Ad (25.37 ± 4.73 MPa) significantly greater than almost all groups (p < 0.05), except for T4/Si/Ad and T5/Si/Ad.In addition, Enamic showed the highest SBS value (28.12 ± 5.45 MPa) followed by Cerasmart and Shofu block HC,respectively. Mixed failure was the most common found in THF with silane and adhesive agent
groups.Conclusion:Tetrahydrofuran with silane and adhesive agent affected to bond strength of RMC materials and resin cements.
Microhardness of light- and dual-cured resin cements after light curing through various translucencies of translucent monolithic zirconia
Authors:   , Pechteewang Sawanya 
Published: September 2023

Purpose: This study aims to investigate the microhardness of light- and dual-cured resin cements under various translucencies of monolithic zirconia specimen; translucent (T), super translucent (ST), high translucent (HT), and extra translucent (XT). Materials and Methods: Four different translucencies of zirconia specimen were prepared in the square shape, size 10×10×1 mm. The light-cured resin cement (Variolink® N LC) and the dual-cured (RelyX™ U200) resin cement were mixed and loaded in a 2-mm height and 5-mm diameter of space in a customized mold and cured through the zirconia specimens and 1-mm thick glass slide in a control group, using the light curing unit for 20 seconds (n=5). All samples were stored dry in a dark room at 37°C for 24 h. The microhardness test was performed on the top of the cement layer with a Vickers hardness tester with a 300-g load. The results of mean and standard deviation of Vickers hardness number (VHN) were recorded and statistically analyzed with two-way ANOVA and post hoc analysis (α=0.05). Results: The mean VHN values of both cements were significantly lower when cured under T group compared to the XT, HT, and ST groups. RelyX™ U200 resin cement showed higher VHN value than Variolink® N LC in all groups. Conclusion: The VHN of both light- and dual-cured resin cement was influenced by different translucencies of zirconia. For both light- and dual-cured resin cements under the lowest translucency of T zirconia showed significantly lower than the other groups.
The experience of the application of safeguards on certain iron or steel products in Indonesia : lesson for Thailand
Authors:   , Santimetvirul Nattanit 
Published: September 2023

In comparison to other trade remedies under WTO, the number of safeguard measures imposed by WTO members has been relatively low as the requirements of safeguard is difficult to fulfill. However, there is change to such trend as there has been an increase in number of safeguard measures imposed by Indonesia. The Appellate Body Report on Indonesia — Safeguard on Certain Iron or Steel Products has provided important lesson to Thailand concerning on the determination of safeguard measure which has not been clearly mentioned in the WTO cases. The importance of safeguard characterization has been emphasized. This dispute has given an significant takeaway that the panel is free to perform objective and independent assessment on the characterization of safeguard measure despite the determination of safeguard measures by domestic authorities. This thesis will address the facts and issues arising in Indonesia — Safeguard on Certain Iron or Steel Products. The underlying reasons and effects resulting from the imposition of specific duty on the imports of galvalume which is unbound product under the Schedule of Concessions will also be examined. More importantly, Indonesia — Safeguard on Certain Iron or Steel Products has given significant implication on the determination of safeguard measure, which will consequently reflect the way the WTO Dispute Settlement Body will handle the application of safeguard measure in the future. Ultimately, this thesis will provide recommendation for Thailand in protecting domestic steel industry.
Effect of different silane coupling agents on the wettability and shear bond strength between glass ceramic and resin cement
Authors:   , Tarateeraseth Tanapon 
Published: September 2023

Silane coupling agents are synthetic hybrid inorganic-organic compounds that act as adhesion promoters which chemically unify dissimilar materials. Nowadays, many brands of silane coupling agent are commercially available in the market. Each brand formulate differently from one another providing the dentists various options to choose from. The aims of this study was to investigate the effect of treating the lithium disilicate glass ceramic surface with various types of silane coupling agents in term of bonding capability and wettability. The experiment was divided into two parts.

The first part of the research was to evaluate the effect of various type of silane coupling agent on the shear bond strength between lithium disilicate glass ceramic and resin cement. Three commercial dental silanes and one experimental silane were used, in vitro, to promote the bonding of resin cement to lithium disilicate glass ceramic surfaces.

The second part of the research was to investigate the effect of treating the hydrofluoric acid-etched/unetched lithium disilicate surface with various types of silane coupling agents on the contact angle measurement between lithium disilicate glass ceramic surface and deionized water. The silane coupling agents used were the same as those in the first part of the study.

The results of this study showed that the type of silane coupling agent used significantly influence the bond strength between lithium disilicate and resin cement and that application of silane coupling agents significantly reduced the wettability of deionized water on the silane-coated surface. The type of silane coupling agent selected significantly influenced the wettability of deionized water. Etching the surface with hydrofluoric acid prior to silane application significantly increased surface wettability in all treatment groups except for groups that were treated with resin-containing silane coupling agent.
A comparison of emotion regulation strategies’ effectiveness under cognitive fatigue
Authors:   , Churassamee Sirinapa 
Published: September 2023

This research compared the effectiveness of three emotion regulation strategies including reappraisal, distraction, and affect labeling under cognitive fatigue. In the 2 (fatigue vs. non-fatigue) × 3 (emotion regulation strategies) within-subject design, 46 participants were randomly assigned into conditions using an incomplete block design method. Self-report negative emotions and skin conductance responses to emotion-eliciting pictures were measured to compare the effectiveness of the strategies. Results showed that reappraisal was more effective in regulating negative emotions than distraction and affect labeling in both fatigue and non-fatigue conditions. While reappraisal was a robust method of regulating emotion, the other two less-demanding strategies show some promising results. The present study provided a consistent conclusion with previous works which showed that reappraisal worked better than distraction and affect labeling. However, no difference in emotional responses was found when comparing the cognitive fatigue conditions.