bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histological assessment of nanostructured fibrin‐agarose skin substitutes grafted in burnt patients. A time‐course study
Authors: Martin‐Piedra Miguel Angel , Carmona Gloria , Campos Fernando , Carriel Víctor , Fernández‐González Ana , Campos Antonio , Cuende Natividad , Garzón Ingrid , Gacto Purificación , Alaminos Miguel 
Published: July 2023

AbstractA previously developed fibrin‐agarose skin model—UGRSKIN—showed promising clinical results in severely burnt patients. To determine the histological parameters associated to the biocompatibility and therapeutic effects of this model, we carried out a comprehensive structural and ultrastructural study of UGRSKIN grafted in severely burnt patients after 3 months of follow‐up. The grafted epidermis was analogue to native human skin from day 30th onward, revealing well‐structured strata with well‐differentiated keratinocytes expressing CK5, CK8, CK10, claudin, plakoglobin, filaggrin, and involucrin in a similar way to controls, suggesting that the epidermis was able to mature and differentiate very early. Melanocytes and Langerhans cells were found from day 30th onward, together with a basement membrane, abundant hemidesmosomes and lack of rete ridges. At the dermal layer, we found an interface between the grafted skin and the host tissue at day 30th, which tended to disappear with time. The grafted superficial dermis showed a progressive increase in properly‐oriented collagen fibers, elastic fibers and proteoglycans, including decorin, similarly to control dermis at day 60‐90th of in vivo follow‐up. Blood vessels determined by CD31 and SMA expression were more abundant in grafted skin than controls, whereas lymphatic vessels were more abundant at day 90th. These results contribute to shed light on the histological parameters associated to biocompatibility and therapeutic effect of the UGRSKIN model grafted in patients and demonstrate that the bioengineered skin grafted in patients is able to mature and differentiate very early at the epithelial level and after 60–90 days at the dermal level.
Should we abandon hormonal therapy in endometrial cancer? Outcomes of recurrent and metastatic endometrial cancer treated with systemic progestins
Authors: Kulkarni A. , Wright N. M. Andrews , Forget A. N. , Ramsay T. , Mallick R. , Weberpals J. I. 
Published: July 2023

AbstractPurposeThe objective of this study is to determine primary survival endpoints in women with recurrent and metastatic endometrial carcinoma (RMEC) treated with progestins.MethodsA retrospective chart review was conducted at The Ottawa Hospital using electronic medical records. Inclusion criteria were a diagnosis of RMEC between 2000 and 2019, endometrioid histology, and ≥one line of progestin treatment. Progression‐free survival (PFS) and overall survival (OS) were estimated using the Kaplan–Meier method.ResultsOf 2342 cases reviewed, 74 met inclusion criteria. Sixty‐six (88.0%) patients received megestrol acetate and 9 (12.0%) received a progestin alternative. The distribution of tumors by grade was: 1: 25 (33.3%), 2: 30 (40.0%), and 3: 20 (26.7%). The PFS and OS for the entire study sample was 14.3 months (95% CI 6.2–17.9) and 23.3 months (14.8–36.8), respectively. The PFS for patients with Grade 1–2 RMEC was 15.7 months (8.0, 19.5), compared to 5.0 months (3.0, 23.0) with Grade 3 disease. The OS for patients with Grade 1–2 versus Grade 3, was 25.9 months (15.3, 40.3) versus 12.5 months (5.7, 35.9), respectively. Thirty‐four (45.9%) and 40 (54.1%) patients were treated with 0 and ≥1 line of chemotherapy. The PFS for chemotherapy‐naïve patients was 17.9 months (14.3, 27.0), versus 6.2 months (3.9, 14.8) following ≥1 line of treatment. The OS was 29.1 months (17.9, 61.1) for chemotherapy‐naïve patients versus 23.0 months (10.5, 37.6) for patients previously exposed.ConclusionsThis real‐world data on RMEC suggests there is a role for progestins in select subgroups of women. The PFS for chemotherapy‐naïve patients was 17.9 months (14.3, 27.0), versus 6.2 months (3.9, 14.8) following ≥1 line of treatment. The OS was 29.1 months (17.9, 61.1) for chemotherapy‐OS was 29.1 months (17.9, 61.1) for chemotherapy‐naïve patients versus 23.0 months (10.5, 37.6) for patients previously exposed.
In Vitro Anthelminthic Activity and Ultrastructural Analysis of Barbatic Acid against Schistosomulae and Juvenile Worms of Schistosoma mansoni**
Authors: Fagundes Silva Hianna Arely Milca , Andrade de Araújo Hallysson Douglas , Pessoa de Azevedo Albuquerque Mônica Camelo , de Vasconcelos Lima Maíra , Barroso Martins Mônica Cristina , Alves Luiz Carlos , Brayner Fábio André , de Lima Aires André , Mendonça de Albuquerque Melo Ana Maria , da Silva Nicácio Henrique 
Published: July 2023

AbstractSchistosomiasis affects about 260 million people worldwide and the search for new schistosomicidal compounds is urgent. In this study we evaluated the in vitro effect of barbatic acid against schistosomulae and young worms of Schistosoma mansoni. The barbatic acid was evaluated through the bioassay of motility and mortality, cellular viability and ultrastructural analysis of juvenile stages through Scanning Electron Microscopy. Barbatic acid showed a schistosomicidal effect against schistosomulae and young worms of S. mansoni after 3 h of exposure. At the end of 24 h, barbatic acid showed 100 %, 89.5 %, 52 % and 28.5 % of lethality for schistosomulae at the concentrations of 200, 100, 50 and 25 μM, respectively. For young worms, barbatic acid showed 100 % and 31.7 % of lethality at the concentrations of 200 and 100 μM, respectively. Motility changes were observed at all sublethal concentrations. There was a significant reduction in the viability of young worms after exposure to barbatic acid at 50, 100 and 200 μM. Extensive damage to the schistosomulae and young worm's tegument, was observed from 50 μM. This report provides data showing the schistosomicidal effect of barbatic acid on schistosomulae and young worms of S. mansoni, causing death, motility changes and ultrastructural damage to worms.
Synthesis and in Silico Study of Novel Benzisoxazole‐Chromene Derivatives as Potent Inhibitors of Acetylcholinesterase: Metal‐Free Site‐Selective C−N Bond Formation via Aza‐Michael Reaction
Authors: Govada Grace Victoria , Rajasekhara Reddy Sabbasani 
Published: July 2023

AbstractAn efficient metal‐free approach for site selective C−N coupling reaction of benzo[d]isoxazole and 2H‐chromene derivatives has been designed and developed against AchE. This nitrogen containing organo‐base promoted methodology, which is both practical and environmentally friendly, provides an easy and suitable pathway for synthesizing Benzisoxazole‐Chromene (BC) possessing poly heteroaryl moieties. The synthesized BC derivatives 4 a–n was docked into the active sites of AChE to obtain more perception into the binding modes of the compounds. Out of them, compound 4 a and 4 l displayed potent activity and high selectivity against the AChE inhibition. Final docking results indicates that compound 4 l showed the lowest binding energy of −11.2260 kcal/mol with AChE. The synthesized BC analogs would be potential candidates for promoting suitable studies in medicinal chemistry research.
Environmentally Friendly Approach to the Synthesis of 3‐[Benzylideneamino]‐2‐methylquinazolin‐4(3H)‐one Derivatives and Calculation of Their Toxicity
Authors: Molnar Maja , Gazivoda Kraljević Tatjana , Pavić Valentina , Rastija Vesna , Komar Mario 
Published: July 2023

AbstractApplication of deep eutectic solvents in synthesis of different heterocyclic compounds was proven very efficient. These solvents are a new generation of green solvents showing excellent potential for different purposes, where they are used as environmentally acceptable substitute for toxic and volatile organic solvents. This research describes their application in the synthesis of series of quinazolinone Schiff bases in combination with microwave, ultrasound‐assisted and mechanochemical methods. First, a model reaction was performed in 20 different deep eutectic solvents to find the best solvent and then reaction conditions (solvent, temperature and reaction time) were optimized for each method. Afterwards, 40 different quinazolinone derivatives were synthesized in choline chloride/malonic acid (1 : 1) DES by each method and compared by their yields. Here we show that deep eutectic solvents can be very efficient in the synthesis of quinazolinone derivatives as an excellent substitution for volatile organic solvents. With green chemistry approach in mind, we have also performed a calculation on compounds’ toxicity and solubility, showing that most of them possess toxic and mutagenic properties with low water solubility.
Synthesis and Evaluation of Novel Metacetamol Derivatives with Hydrazone Moiety as Anticancer and Antimicrobial Agents
Authors: Şenkardeş Sevil , Atlıhan İrem , Çayır Elif , Mega Tiber Pınar , Orun Oya , Nigiz Şeyma , Özkul Ceren , Gündüz Miyase Gözde , Küçükgüzel Ş. Güniz 
Published: July 2023

AbstractBy exploiting the wide biological potential of the hydrazone scaffold, a series of hydrazone derivatives were synthesized, starting from N‐(3‐hydroxyphenyl)acetamide (metacetamol). The structures of the compounds were determined using IR, 1H and 13C‐NMR, and mass spectroscopic methods. The obtained molecules (3 a–j) were evaluated for their anticancer potential against MDA‐MB‐231 and MCF‐7 breast cancer cell lines. According to the CCK‐8 assay, all tested compounds showed moderate to potent anticancer activity. Among them, N‐(3‐(2‐(2‐(4‐nitrobenzylidene)hydrazinyl)‐2‐oxoethoxy)phenyl)acetamide (3 e) was found to be the most effective derivative with an IC50 value of 9.89 μM against MDA‐MB‐231 cell lines. This compound was further tested for its potential effects on the apoptotic pathway. Molecular docking studies was also carried out for 3 e in the colchicine binding pocket of tubulin. Additionally, compound 3 e also demonstrated effective antifungal activity, particularly against Candida krusei (MIC=8 μg/ml), indicating that nitro group at the 4th position of the phenyl ring was the most preferable substituent for both cytotoxic and antimicrobial activity. Our preliminary findings suggest that compound 3 e could be exploited as a leading structure for further anticancer and antifungal drug development.
A Modular Chemistry Platform for the Development of a Cereblon E3 Ligase‐Based Partial PROTAC Library
Authors: Almodóvar‐Rivera Chelsi M. , Zhang Zhen , Li Jingyao , Xie Haibo , Zhao Yu , Guo Le , Mannhardt Marissa G. , Tang Weiping 
Published: July 2023

AbstractProteolysis targeting chimeras (PROTACs) are a promising therapeutic strategy to selectively promote the degradation of protein targets by exploiting the ubiquitin‐proteasome system. Among the limited number of E3 ligase ligands discovered for the PROTAC technology, ligands of cereblon (CRBN) E3 ligase, such as pomalidomide, thalidomide, or lenalidomide, are the most frequently used for the development of PROTACs. Our group previously reported that a phenyl group could be tolerated on the C4‐position of lenalidomide as the ligand of CRBN to develop PROTACs. Herein, we report a modular chemistry platform for the efficient attachment of various ortho‐, meta‐, and para‐substituted phenyls to the C4‐position of the lenalidomide via Suzuki cross‐coupling reaction, which allows the systematic investigation of the linker effect for the development of PROTACs against any target. We examined the substrate scope by preparing twelve lenalidomide‐derived CRBN E3 ligase ligands with different linkers.
Comparison of drug‐coated balloon angioplasty versus common balloon angioplasty for arteriovenous fistula stenosis: A systematic review and meta‐analysis
Authors: Zhang Yong , Yuan Fan‐Li , Hu Xiang‐Yang , Wang Qi‐Bing , Zou Zhen‐Wu , Li Zhen‐Guo 
Published: July 2023

AbstractDrug‐coated balloons (DCBs) have been used in dialysis patients with arteriovenous fistula (AVF) stenosis, but whether DCBs have advantages over ordinary balloons is still controversial. A meta‐analysis was designed to investigate the safety and efficacy of DCBs and common balloons (CBs) in the treatment of AVF stenosis. We searched the PubMed, EMBASE, and China National Knowledge Internet (CNKI) databases for randomized controlled trials that evaluated the comparison of DCB angioplasty versus CB angioplasty for AVF stenosis in dialysis patients and reported at least one outcome of interest. The results showed that the DCB group had a higher first‐stage patency rate of the target lesion 6 months [odds ratio, OR = 2.31, 95% confidence interval, CI: (1.69, 3.15), p < .01] and 12 months [OR = 2.09, 95% CI: (1.50, 2.91), p < .01] after surgery. There was no statistically significant difference in all‐cause mortality between the two groups at 6 months [OR = 0.85, 95% CI: (0.47, 1.52), p = .58] and 12 months [OR = 0.99, 95% CI: (0.60, 1.64), p = .97]. Compared with CB, DCBs as a new endovascular treatment for AVF stenosis have a higher primary patency rate of target lesions and can delay the occurrence of restenosis. There is no evidence that DCB can increase the mortality of patients.
K‐means cluster analysis of characteristic patterns of allergen in different ages: Real life study
Authors: Zhao Lei , Fang Jie , Ji Yong , Zhang Yingying , Zhou Xin , Yin Junfeng , Zhang Min , Bao Wuping 
Published: July 2023

AbstractBackgroundAtopy varies in people of different ages owing to different physical conditions and exposure to allergens. We aimed to cluster ages based on atopic severity using K‐means cluster analysis and identify atopic incidence, severity, as well as the association among peripheral eosinophils, IgE and sensitisation.MethodsConsecutive patients (n = 7654) with allergic symptoms and undergoing allergen‐specific IgE tests were included from 2013 to 2017. Age, sex, specific‐IgE, peripheral eosinophil counts and total‐IgE were collected.ResultsFive age categories were identified: 1–17, 18–36, 37–52, 53–69 and 70–100 years. The incidences of atopy and poly‐sensitisation decreased with increasing age. Similar trend was observed for aeroallergens, egg and milk but not for peanuts, soy or seafood. Dust mites remain the crucial factor bothering patients with allergic symptoms, especially for children and adolescents. In patients aged <52 years, sensitisation to aeroallergens was more prevalent than food. In group 37–52 years, incidence of females' atopy was higher than that of males. The overlap of atopy, high eosinophils, and high total‐IgE was found in only 19.18% of patients. The trend of allergen‐test positivity is not parallel to total IgE and peripheral eosinophil counts.ConclusionAge‐grouping based on cluster analysis helps to find the changes in atopic status and distribution of sensitised allergens with age. Allergen tests are still necessary in the clinical diagnosis and treatment. An innovative exploration of the influence of age and allergens on total‐IgE and eosinophil counts is helpful for the development of bio‐targeted precision therapy.Clinical Trial RegistrationChiCTR2300067700.
Digital Examination of LYmph node CYtopathology Using the Sydney system (DELYCYUS): An international, multi‐institutional study
Authors: Caputo Alessandro , Fraggetta Filippo , Cretella Pasquale , Cozzolino Immacolata , Eccher Albino , Girolami Ilaria , Marletta Stefano , Troncone Giancarlo , Vigliar Elena , Acanfora Gennaro , Zarra Karen Villar , Torres Rivas Héctor Enrique , Fadda Guido , Field Andrew , Katz Ruth , Vielh Philippe , Eloy Catarina , Rajwanshi Arvind , Gupta Nalini , Al‐Abbadi Mousa , Bustami Nadwa , Arar Tala , Calaminici Maria , Raine Juliet I. , Barroca Helena , Canão Pedro Amoroso , Ehinger Mats , Rajabian Nilofar , Dey Pranab , Medeiros L. Jeffrey , El Hussein Siba , Lin Oscar , D’Antonio Antonio , Bode‐Lesniewska Beata , Rossi Esther Diana , Zeppa Pio 
Published: July 2023

AbstractBackgroundAfter a series of standardized reporting systems in cytopathology, the Sydney system was recently introduced to address the need for reproducibility and standardization in lymph node cytopathology. Since then, the risk of malignancy for the categories of the Sydney system has been explored by several studies, but no studies have yet examined the interobserver reproducibility of the Sydney system.MethodsThe authors assessed interobserver reproducibility of the Sydney system on 85 lymph node fine‐needle aspiration cytology cases reviewed by 15 cytopathologists from 12 institutions in eight different countries, resulting in 1275 diagnoses. In total, 186 slides stained with Diff‐Quik, Papanicolaou, and immunocytochemistry were scanned. A subset of the cases included clinical data and results from ultrasound examinations, flow cytometry immunophenotyping, and fluorescence in situ hybridization analysis. The study participants assessed the cases digitally using whole‐slide images.ResultsOverall, the authors observed an almost perfect agreement of cytopathologists with the ground truth (median weighted Cohen κ = 0.887; interquartile range, κ = 0.210) and moderate overall interobserver concordance (Fleiss κ = 0.476). There was substantial agreement for the inadequate and malignant categories (κ = 0.794 and κ = 0.729, respectively), moderate agreement for the benign category (κ = 0.490), and very slight agreement for the suspicious (κ = 0.104) and atypical (κ = 0.075) categories.ConclusionsThe Sydney system for reporting lymph node cytopathology shows adequate interobserver concordance. Digital microscopy is an adequate means to assess lymph node cytopathology specimens.
Pharmacokinetic Similarity of ABP 654, an Ustekinumab Biosimilar Candidate: Results from a Randomized, Double‐blind Study in Healthy Subjects
Authors: Chow Vincent , Mytych Daniel T. , Das Shyamal , Franklin Janet 
Published: July 2023

AbstractABP 654 is a proposed biosimilar to ustekinumab reference product (RP) which works through antagonism of interleukin‐12 and interleukin‐23. Ustekinumab RP is used for the treatment of chronic inflammatory conditions, including some forms of plaque psoriasis, psoriatic arthritis, Crohn's disease, and ulcerative colitis. A randomized, double‐blinded, single‐dose, 3‐arm, parallel‐group study was conducted to assess the pharmacokinetic (PK) similarity of ABP 654 with ustekinumab RP sourced from the United States (US) and the European Union (EU); the PK similarity of ustekinumab US versus ustekinumab EU; and the comparative safety, tolerability, and immunogenicity of all 3 products. A total of 238 healthy subjects were randomized 1:1:1 and stratified by gender and ethnicity (Japanese versus non‐Japanese) to receive a single 90 mg subcutaneous injection of ABP 654 or ustekinumab US or ustekinumab EU. PK similarity was established based on 90% confidence intervals (CIs) for the primary endpoints of area under the concentration‐time curve from time 0 extrapolated to infinity (AUCinf) and maximum observed serum concentration (Cmax) being contained within the prespecified margin of 0.8–1.25. No clinically meaningful differences in immunogenicity were found among the 3 products. Adverse events were similar between treatment groups and consistent with the safety profile of ustekinumab RP. Results indicate that ABP 654, ustekinumab US and ustekinumab EU share similar PK and safety profiles.
Mapping tree‐shaped workflows on systems with different memory sizes and processor speeds
Authors: Kulagina Svetlana , Meyerhenke Henning , Benoit Anne 
Published: July 2023

AbstractDirected acyclic graphs are commonly used to model scientific workflows, by expressing dependencies between tasks, as well as the resource requirements of the workflow. As a special case, rooted directed trees occur in several applications, for instance in sparse matrix computations. Since typical workflows are modeled by large trees, it is crucial to schedule them efficiently, so that their execution time (or makespan) is minimized. Furthermore, it is usually beneficial to distribute the execution on several compute nodes, hence increasing the available memory, and allowing us to parallelize parts of the execution. To exploit the heterogeneity of modern clusters in this context, we investigate the partitioning and mapping of tree‐shaped workflows on two types of target architecture models: in AM1, each processor can have a different memory size, and in AM2, each processor can also have a different speed (in addition to a different memory size). We design a three‐step heuristic for AM1, which adapts and extends previous work for homogeneous clusters [Gou C, Benoit A, Marchal L. Partitioning tree‐shaped task graphs for distributed platforms with limited memory. IEEE Trans Parallel Dist Syst 2020; 31(7): 1533–1544]. The changes we propose concern the assignment to processors (accounting for the different memory sizes) and the availability of suitable processors when splitting or merging subtrees. For AM2, we extend the heuristic for AM1 with a two‐phase local search approach. Phase A is a swap‐based hill climber, while (the optional) Phase B is inspired by iterated local search. We evaluate our heuristics for AM1 and AM2 with extensive simulations, and we demonstrate that exploiting the heterogeneity in the cluster significantly reduces the makespan, compared to the state of the art for homogeneous processors.
Terrorist attacks and environmental social and governance performance: Evidence from cross‐country panel data
Authors: Al Amosh Hamzeh , Khatib Saleh F. A. , Ananzeh Husam 
Published: July 2023

AbstractThis study is motivated by the absence of similar research that investigates the link between terrorism level and social and environmental performance measured by the ESG score. This study adopts cross‐country panel data represented by 5082 worldwide companies during the period 2011 to 2020. The terrorism level has been measured using the Global Terrorism Index as reported by The Institute for Economics and Peace Limited. Our findings indicate that the occurrence of terrorist events has a significant negative impact on ESG performance, both collectively and individually, due to the various consequences. Many businesses may suspend operations in countries where the threat of terrorism is increasing, resulting in a flight of investors and the disruption of development projects, which may weaken overall economic performance and voluntary projects. Thus, it is likely to result in undermining companies' environmental and social practices. In addition, as fears of terrorist attacks grow, the general system of corporate governance may deteriorate, halting sustainable development initiatives in these countries. Terrorist attacks introduce complex moral dilemmas, challenge stakeholder priorities, affect a company's legitimacy, and reshape the institutional landscape. Companies must navigate these multifaceted challenges, balancing immediate stakeholder concerns, reputation restoration, compliance obligations, and evolving societal expectations. Our findings are significant, with numerous implications for different stakeholders such as regulators, policymakers, investors, managers, and government agencies.
Effect of Temperature and H Flux on the NH3 Synthesis via Electrochemical Hydrogen Permeation
Authors: Ripepi Davide , Schreuders Herman , Mulder Fokko M. 
Published: July 2023

AbstractInvited for this month′s cover is the group of Prof. Fokko M. Mulder at the Delft University of Technology. The image on the cover shows how in the NH3 synthesis via hydrogen‐permeable electrode the N, H species on the catalyst surface can be controlled, using the analogy of a traffic controller. The Research Article itself is available at 10.1002/cssc.202300460.