bluuebunny/crossref_metadata_2025_split huggingface dataset:
De novo transcriptome assembly and gene annotation for the toxic dinoflagellate Dinophysis
Authors: Gaonkar Chetan C. , Campbell Lisa 
Published: June 2023

AbstractSpecies within the dinoflagellate genus Dinophysis can produce okadiac acid and dinophysistoxins leading to diarrhetic shellfish poisoning. Since the first report of D. ovum from the Gulf of Mexico in 2008, reports of other Dinophysis species across US have increased. Members of the D. cf. acuminata complex (D. acuminata, D. acuta, D. ovum, D. sacculus) are difficult to differentiate due to their morphological similarities. Dinophysis feeds on and steals the chloroplasts from the ciliate, Mesodinium rubrum, which in turn has fed on and captured the chloroplasts of its prey, the cryptophyte Teleaulax amphioxeia. The objective of this study was to generate de novo transcriptomes for new isolates of these mixotrophic organisms. The transcriptomes obtained will serve as a reference for future experiments to assess the effect of different abiotic and biotic conditions and will also provide a useful resource for screening potential marker genes to differentiate among the closely related species within the D. cf. acuminata-complex. The complete comprehensive detailed workflow and links to obtain the transcriptome data are provided.
Simulated sulfur K-edge X-ray absorption spectroscopy database of lithium thiophosphate solid electrolytes
Authors: Guo Haoyue , Carbone Matthew R. , Cao Chuntian , Qu Jianzhou , Du Yonghua , Bak Seong-Min , Weiland Conan , Wang Feng , Yoo Shinjae , Artrith Nongnuch , Urban Alexander , Lu Deyu 
Published: June 2023

AbstractX-ray absorption spectroscopy (XAS) is a premier technique for materials characterization, providing key information about the local chemical environment of the absorber atom. In this work, we develop a database of sulfur K-edge XAS spectra of crystalline and amorphous lithium thiophosphate materials based on the atomic structures reported in Chem. Mater., 34, 6702 (2022). The XAS database is based on simulations using the excited electron and core-hole pseudopotential approach implemented in the Vienna Ab initio Simulation Package. Our database contains 2681 S K-edge XAS spectra for 66 crystalline and glassy structure models, making it the largest collection of first-principles computational XAS spectra for glass/ceramic lithium thiophosphates to date. This database can be used to correlate S spectral features with distinct S species based on their local coordination and short-range ordering in sulfide-based solid electrolytes. The data is openly distributed via the Materials Cloud, allowing researchers to access it for free and use it for further analysis, such as spectral fingerprinting, matching with experiments, and developing machine learning models.
A new remarkable Early Cretaceous nelumbonaceous fossil bridges the gap between herbaceous aquatic and woody protealeans
Authors: Gobo William Vieira , Kunzmann Lutz , Iannuzzi Roberto , dos Santos Thamiris Barbosa , da Conceição Domingas Maria , Rodrigues do Nascimento Daniel , da Silva Filho Wellington Ferreira , Bachelier Julien B. , Coiffard Clément 
Published: June 2023

AbstractDating back to the late Early Cretaceous, the macrofossil record of the iconic lotus family (Nelumbonaceae) is one of the oldest of flowering plants and suggests that their unmistakable leaves and nutlets embedded in large pitted receptacular fruits evolved relatively little in the 100 million years since their first known appearance. Here we describe a new fossil from the late Barremian/Aptian Crato Formation flora (NE Brazil) with both vegetative and reproductive structures, Notocyamus hydrophobus gen. nov. et sp. nov., which is now the oldest and most complete fossil record of Nelumbonaceae. In addition, it displays a unique mosaic of ancestral and derived macro- and micromorphological traits that has never been documented before in this family. This new Brazilian fossil-species also provides a rare illustration of the potential morphological and anatomical transitions experienced by Nelumbonaceae prior to a long period of relative stasis. Its potential plesiomorphic and apomorphic features shared with Proteaceae and Platanaceae not only fill a major morphological gap within Proteales but also provide new support for their unexpected relationships first suggested by molecular phylogenies.
Growth patterns in children and adolescents with cerebral palsy from Argentina and Germany
Authors: Ruiz Brunner Maria de las Mercedes , Cuestas Eduardo , von Kries Rüdiger , Brooks Jordan , Wright Charlotte , Heinen Florian , Schroeder Andreas Sebastian 
Published: June 2023

AbstractTo analyze growth patterns of children with CP between countries; to examine differences in growth; and to assess the fit of growth charts. Cross-sectional study in children with CP from 2 to 19 years old, 399 from Argentina and 400 from Germany. Growth measures were converted into z-scores and compared to WHO reference and US CP growth charts. Generalized Linear Model was used to analyze the growth expressed as mean z-scores. 799 children. Mean age 9 years (± 4). Compared to the WHO reference, the decrease in Height z-scores (HAZ) with age in Argentina (− 0.144/year) was double that in Germany (− 0.073/year). For children in GMFCS IV–V, BMI z-scores (BMIZ) decreased with age (− 0.102/year). Using the US CP charts, both countries showed decreasing HAZ with age, in Argentina (− 0.066/year) and in Germany (− 0.032/year). BMIZ increased more among children with feeding tubes (0.062/year), similar in both countries. Argentinian children with oral feeding decrease their Weight z-score (WAZ) by − 0.553 compared to their peers. With WHO charts BMIZ presented an excellent fit for GMFCS I–III. HAZ presents a poor fit to growth references. BMIZ and WAZ presented a good fit to US CP Charts. Growth differences due to ethnicity also act in children with CP, and are related to motor impairment, age and feeding modality, possibly reflecting differences in environment or health care.
Fabrication and characterization of graphene oxide-based polymer nanocomposite coatings, improved stability and hydrophobicity
Authors: Kumar Sachin Sharma Ashok , M. Nujud Badawi , Batoo Khalid Mujasam , Wonnie Ma I. A. , Ramesh K. , Ramesh S. , Shah Mohd Asif 
Published: June 2023

AbstractIn this study, acrylic-epoxy-based nanocomposite coatings loaded with different concentrations (0.5–3 wt.%) of graphene oxide (GO) nanoparticles were successfully prepared via the solution intercalation approach. The thermogravimetric analysis (TGA) revealed that the inclusion of GO nanoparticles into the polymer matrix increased the thermal stability of the coatings. The degree of transparency evaluated by the ultraviolet–visible (UV–Vis) spectroscopy showed that the lowest loading rate of GO (0.5 wt.%) had completely blocked the incoming irradiation, thus resulting in zero percent transmittance. Furthermore, the water contact angle (WCA) measurements revealed that the incorporation of GO nanoparticles and PDMS into the polymer matrix had remarkably enhanced the surface hydrophobicity, exhibiting the highest WCA of 87.55º. In addition, the cross-hatch test (CHT) showed that all the hybrid coatings exhibited excellent surface adhesion behaviour, receiving 4B and 5B ratings respectively. Moreover, the field emission scanning electron microscopy (FESEM) micrographs confirmed that the presence of the functional groups on the GO surface facilitated the chemical functionalization process, which led to excellent dispersibility. The GO composition up to 2 wt.% showed excellent dispersion and uniform distribution of the GO nanoparticles within the polymer matrix. Therefore, the unique features of graphene and its derivatives have emerged as a new class of nanofillers/inhibitors for corrosion protection applications.
Assessing the representational structure of softness activated by words
Authors: Cavdan Müge , Goktepe Nedim , Drewing Knut , Doerschner Katja 
Published: June 2023

AbstractSoftness is an important material property that can be judged directly, by interacting with an object, but also indirectly, by simply looking at an image of a material. The latter is likely possible by filling in relevant multisensory information from prior experiences with soft materials. Such experiences are thought to lead to associations that make up our representations about perceptual softness. Here, we investigate the structure of this representational space when activated by words, and compare it to haptic and visual perceptual spaces that we obtained in earlier work. To this end, we performed an online study where people rated different sensory aspects of soft materials, presented as written names. We compared the results with the previous studies where identical ratings were made on the basis of visual and haptic information. Correlation and Procrustes analyses show that, overall, the representational spaces of verbally presented materials were similar to those obtained from haptic and visual experiments. However, a classifier analysis showed that verbal representations could better be predicted from those obtained from visual than from haptic experiments. In a second study we rule out that these larger discrepancies in representations between verbal and haptic conditions could be due to difficulties in material identification in haptic experiments. We discuss the results with respect to the recent idea that at perceived softness is a multidimensional construct.
No impacts of glyphosate or Crithidia bombi, or their combination, on the bumblebee microbiome
Authors: Straw Edward A. , Mesnage Robin , Brown Mark J. F. , Antoniou Michael N. 
Published: June 2023

AbstractPesticides are recognised as a key threat to pollinators, impacting their health in many ways. One route through which pesticides can affect pollinators like bumblebees is through the gut microbiome, with knock-on effects on their immune system and parasite resistance. We tested the impacts of a high acute oral dose of glyphosate on the gut microbiome of the buff tailed bumblebee (Bombus terrestris), and glyphosate’s interaction with the gut parasite (Crithidia bombi). We used a fully crossed design measuring bee mortality, parasite intensity and the bacterial composition in the gut microbiome estimated from the relative abundance of 16S rRNA amplicons. We found no impact of either glyphosate, C. bombi, or their combination on any metric, including bacterial composition. This result differs from studies on honeybees, which have consistently found an impact of glyphosate on gut bacterial composition. This is potentially explained by the use of an acute exposure, rather than a chronic exposure, and the difference in test species. Since A. mellifera is used as a model species to represent pollinators more broadly in risk assessment, our results highlight that caution is needed in extrapolating gut microbiome results from A. mellifera to other bee species.
A single-phase direct buck-boost AC–AC converter with minimum number of components
Authors: Adel Fawzy , Lashine Azza E. , El-Sabbe Awad E. , Osheba Dina S. M. 
Published: June 2023

AbstractIn this paper, a single-phase direct pulse width modulation (PWM) buck-boost AC–AC converter is proposed. The proposed converter utilizes a minimum number of semiconductor switches and passive components that decreases the converter power losses and offers high efficiency. It can be operated with simple PWM control and doesn’t require soft-commutation strategies. It does not suffer from input source shoot-through and commutation problems. Moreover, it supplies both continuous input and output currents. The common sharing ground of the input and output gives the proposed converter the feature that it can be utilized for voltage sag and swell compensation. A comparison of the proposed converter performance with similar existing converters is presented. Also, detailed circuit analysis, component design guidelines, and simulation results using the MATLAB/Simulink environment are demonstrated. A laboratory prototype has been built and tested to validate the converter performance and confirm the results obtained by computer simulation.
Biosynthesis of Co3O4 nanomedicine by using Mollugo oppositifolia L. aqueous leaf extract and its antimicrobial, mosquito larvicidal activities
Authors: Gowthami P. , Kosiha A. , Meenakshi S. , Boopathy G. , Ramu A. G. , Choi Dongjin 
Published: June 2023

AbstractNanotechnology is a relatively revolutionary area that generates day-to-day advancement. It makes a significant impact on our daily life. For example, in parasitology, catalysis and cosmetics, nanoparticles possess distinctive possessions that make it possible for them in a broad range of areas. We utilized Mollugo oppositifolia L. aqueous leaf extract assisted chemical reduction method to synthesize Co3O4 nanoparticles. Biosynthesized Co3O4 Nps were confirmed via UV–Vis spectroscopy, scanning electron microscope, X-ray diffraction, EDX, Fourier-transform infrared, and HR-TEM analysis. The crystallite size from XRD studies revealed around 22.7 nm. The biosynthesized Co3O4 nanoparticle was further assessed for mosquito larvicidal activity against south-urban mosquito larvae Culex quinquefasciatus, and antimicrobial activities. The synthesized Co3O4 particle (2) displayed significant larvicidal activity towards mosquito larvae Culex quinquefasciatus with the LD50 value of 34.96 µg/mL than aqueous plant extract (1) and control Permethrin with the LD50 value of 82.41 and 72.44 µg/mL. When compared to the standard antibacterial treatment, Ciprofloxacin, the Co3O4 nanoparticle (2) produced demonstrates significantly enhanced antibacterial action against the pathogens E. coli and B. cereus. The MIC for Co3O4 nanoparticles 2 against C. albicans was under 1 μg/mL, which was much lower than the MIC for the control drug, clotrimale, which was 2 µg per milliliter. Co3O4 nanoparticles 2, with a MIC of 2 μg/mL, has much higher antifungal activity than clotrimale, whose MIC is 4 μg/mL, against M. audouinii.
Combination of triheptanoin with the ketogenic diet in Glucose transporter type 1 deficiency (G1D)
Authors: Avila Adrian , Málaga Ignacio , Sirsi Deepa , Kayani Saima , Primeaux Sharon , Kathote Gauri A. , Jakkamsetti Vikram , Kallem Raja Reddy , Putnam William C. , Park Jason Y. , Shinnar Shlomo , Pascual Juan M. 
Published: June 2023

AbstractFuel influx and metabolism replenish carbon lost during normal neural activity. Ketogenic diets studied in epilepsy, dementia and other disorders do not sustain such replenishment because their ketone body derivatives contain four carbon atoms and are thus devoid of this anaplerotic or net carbon donor capacity. Yet, in these diseases carbon depletion is often inferred from cerebral fluorodeoxyglucose-positron emission tomography. Further, ketogenic diets may prove incompletely therapeutic. These deficiencies provide the motivation for complementation with anaplerotic fuel. However, there are few anaplerotic precursors consumable in clinically sufficient quantities besides those that supply glucose. Five-carbon ketones, stemming from metabolism of the food supplement triheptanoin, are anaplerotic. Triheptanoin can favorably affect Glucose transporter type 1 deficiency (G1D), a carbon-deficiency encephalopathy. However, the triheptanoin constituent heptanoate can compete with ketogenic diet-derived octanoate for metabolism in animals. It can also fuel neoglucogenesis, thus preempting ketosis. These uncertainties can be further accentuated by individual variability in ketogenesis. Therefore, human investigation is essential. Consequently, we examined the compatibility of triheptanoin at maximum tolerable dose with the ketogenic diet in 10 G1D individuals using clinical and electroencephalographic analyses, glycemia, and four- and five-carbon ketosis. 4 of 8 of subjects with pre-triheptanoin beta-hydroxybutyrate levels greater than 2 mM demonstrated a significant reduction in ketosis after triheptanoin. Changes in this and the other measures allowed us to deem the two treatments compatible in the same number of individuals, or 50% of persons in significant beta-hydroxybutyrate ketosis. These results inform the development of individualized anaplerotic modifications to the ketogenic diet.ClinicalTrials.gov registration NCT03301532, first registration: 04/10/2017.
Quantifiable peptide library bridges the gap for proteomics based biomarker discovery and validation on breast cancer
Authors: Kim Sung-Soo , Shin HyeonSeok , Ahn Kyung-Geun , Park Young-Min , Kwon Min-Chul , Lim Jae-Min , Oh Eun-Kyung , Kim Yumi , Han Seung-Man , Noh Dong-Young 
Published: June 2023

AbstractMass spectrometry (MS) based proteomics is widely used for biomarker discovery. However, often, most biomarker candidates from discovery are discarded during the validation processes. Such discrepancies between biomarker discovery and validation are caused by several factors, mainly due to the differences in analytical methodology and experimental conditions. Here, we generated a peptide library which allows discovery of biomarkers in the equal settings as the validation process, thereby making the transition from discovery to validation more robust and efficient. The peptide library initiated with a list of 3393 proteins detectable in the blood from public databases. For each protein, surrogate peptides favorable for detection in mass spectrometry was selected and synthesized. A total of 4683 synthesized peptides were spiked into neat serum and plasma samples to check their quantifiability in a 10 min liquid chromatography-MS/MS run time. This led to the PepQuant library, which is composed of 852 quantifiable peptides that cover 452 human blood proteins. Using the PepQuant library, we discovered 30 candidate biomarkers for breast cancer. Among the 30 candidates, nine biomarkers, FN1, VWF, PRG4, MMP9, CLU, PRDX6, PPBP, APOC1, and CHL1 were validated. By combining the quantification values of these markers, we generated a machine learning model predicting breast cancer, showing an average area under the curve of 0.9105 for the receiver operating characteristic curve.
Observed hydrological changes associated with active tectonic blocks before three consecutive earthquakes in Qinghai, China
Authors: Yu Huaizhong , Liu Lei , Ma Yuchuan , Yan Rui , Liu Jie , Ma Yawei , Li Zeping , Zhang Xiaotao , Zhao Jing , Yu Chen 
Published: June 2023

AbstractIn the past 2 years, three earthquakes of magnitude 6.0 and above occurred consecutively in Qinghai province, China, i.e., the 22 May 2021 Ms7.4 Maduo, 8 January 2022 Ms6.9 Menyuan, and 26 March 2022 Ms6.0 Delingha earthquakes. The hydrological observation instruments set up by the China Earthquake Administration allow us to study the dynamic processes in the well-aquifer systems during the establishment of criticality. Particularly, the observations played an important role in the prediction of the 8 January 2022 Ms6.9 Menyuan earthquake that was approved by the People’s Government of Qinghai province. This work presents the hydrological data recorded by 7 stations to show the short-term anomalies before these earthquakes. To explore the performance of the hydrological observations in detecting earthquakes that occurred on different active tectonic blocks, we calculate the relative amplitudes of the pre-seismic changes. Results indicate that markedly pre-seismic change is found if the observation station and the detection earthquake are on the same block, and moderate change is found if they are on the adjacent blocks, while the precursor is hard to be identified if they are on the separated blocks. The variations in the hydrological responses may be caused by the strength weakening (or dilatancy) of source media. And the increased volumes in the crust can be evidenced by the changes in the geodetic time series in the same neighborhoods and during the same period, augmenting stress loading between the blocks.
Interlayer angle control of the electronic mini-gaps, band splitting, and hybridization in graphene–WS2 moiré heterostructures
Authors: Hidalgo Francisco , Sánchez-Ochoa Francisco , Noguez Cecilia 
Published: June 2023

AbstractUnderstanding the electronic properties modulation in graphene/tungsten disulfide (G-WS2) at different interlayer angles is essential for promising building blocks of two-dimensional (2D) heterostructures. G-WS2 heterostructures with four different interlayer angles are studied using periodic first-principles calculations and an unfolding method to decipher the supercell crowded-band structure. Electronic mini-gaps of different sizes, band splittings, and band hybridizations are identified because of the interactions between out-of-plane orbitals from both layers. These electronic changes are modulated depending on the interlayer angle in a given energy window and space region. At the same time, the replicas emerging because of the superperiodic potential associated with moiré patterns also modify such electronic alterations, inducing new electronic repulsions or avoided crossings. Therefore, the number of mini-gaps as well as their energy values and positions are intrinsically related to the interlayer angle. Finally, it is anticipated that these results might be essential for designing the electronic properties of 2D heterostructures.
Investigating the operation mechanism of light-emitting electrochemical cells through operando observations of spin states
Authors: Katsumata Junya , Osawa Fumiya , Sato Go , Sato Atsushi , Miwa Kazumoto , Ono Shimpei , Marumoto Kazuhiro 
Published: June 2023

AbstractLight-emitting electrochemical cells (LECs) are next-generation devices that are flexible, emit light and have several advantages over organic light-emitting diodes, such as a simpler structure and lower cost. However, the operation mechanism of LECs remains unknown from a microscopic viewpoint. Here, we perform an operando microscopic investigation of LECs with Super Yellow, a typical light-emitting material, by observing the spin states of electrically doped charges using electron spin resonance. The operando electron spin resonance and light emission increase as the voltage applied to the LECs increases. Through density functional theory, we determine that the origin of the electron spin resonance increases to be from electrochemically doped holes and electrons in Super Yellow. We find that the doping progress correlates with the luminance increase, suggesting that electrochemically doped charges are distributed over the light-emitting layer as the operation mechanism. Moreover, we deduce the molecular orientation of electrochemically charge-doped Super Yellow.
Degree conditions for path-factor critical deleted or covered graphs
Authors: Liu Hongxia 
Published: June 2023

A path-factor of a graph G is a spanning subgraph of G whose components are paths. A P≥d-factor of a graph G is a path-factor of G whose components are paths with at least d vertices, where d is an integer with d ≥ 2. A graph G is P≥d-factor covered if for any e ∈ E(G), G admits a P≥d-factor including e. A graph G is (P≥d, n)-factor critical deleted if for any Q ⊆ V(G) with |Q| = n and any e ∈ E(G − Q), G – Q − e has a P≥d-factor. A graph G is (P≥d, n)-factor critical covered if for any Q ⊆ V(G) with |Q| = n, G − Q is a P≥d-factor covered graph. In this paper, we verify that (1) an (n + t + 2)-connected graph G of order p with p ≥ 4t + n + 7 is (P≥3, n)-factor critical deleted if max $ \left\{{d}_G({v}_1),{d}_G({v}_2),\dots,{d}_G({v}_{2t+1})\right\}\ge \frac{p+2n}{3}$ for any independent set {v1, v2, …, v2t+1} of G, where n and t are two nonnegative integers with t ≥ 1; (2) an (n + t + 1)-connected graph G of order p with p ≥ 4t + n + 5 is (P≥3, n)-factor critical covered if max $ \left\{{d}_G({v}_1),{d}_G({v}_2),\dots,{d}_G({v}_{2t+1})\right\}\ge \frac{p+2n+2}{3}$ for any independent set {v1, v2, …, v2t+1} of G, where n and t are two nonnegative integers with t ≥ 1.