bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Pemahaman Dan Praktik Makna Frasa “Istri Tunduk Kepada Suami” Berdasarkan Efesus 5:22-33 Terhadap Keharmonisan Rumah Tangga Kristen Di Gereja Bethel Indonesia Jemaat “Kristus Gembala” Juwana–Pati Jawa-Tengah
Authors: Widodo Agus 
Published: August 2023

The purpose of this study was to reveal and analyze the effect on the understanding and practice of “Wives obey Your Husband” based on Ephesians 5:22-33 through the harmony’s Christian household on Indonesian Bethel Church ‘Kristus Gembala’ Juwana-Pati Central java. The method used in this research is a survey research with quantitative research approach. As for the population in this research is all husband and wife on the Indonesian Bethel Church ‘Kristus Gembala’ Juwana-Pati Central java. The results showed that the understanding and practice of “Wives obey your husbans” influence the positive and significant impact on the harmony’s Christian household on Indonesian Bethel Church ‘Kristus Gembala’ Juwana-Pati Central java. The description of understanding and practice show good results.
Abstrak Indonesia

Tujuan penelitian ini adalah mengungkapkan dan menganalisis pengaruh pemahaman dan praktik makna frasa ‘Istri Tunduk kepada Suami’ Berdasarkan Efesus 5:22-33 terhadap keharmonisan Rumah Tangga Kristen di Gereja Bethel Indonesia Jemaat ‘Kristus Gembala’ Juwana-Pati Jawa Tengah. Metode yang digunakan dalam penelitian ini yaitu penelitian survei dengan pendekatan penelitian kuantitatif. Adapun yang menjadi populasi pada penelitian ini yaitu seluruh pasangan suami istri di Gereja Bethel Indonesia Jemaat ‘Kristus Gembala’ Juwana-Pati Jawa-Tengah. Hasil penelitian menunjukkan bahwa pemahaman dan praktik istri tunduk kepada suami berpengaruh positif dan signifikan terhadap keharmonisan rumah tangga Kristen di Gereja Bethel Indonesia Jemaat ‘Kristus Gembala’ Juwana Pati Jawa Tengah. Deskripsi pemahaman dan praktik menunjukkan hasil yang baik. Begitu pun dengan deskripsi keharmonisan rumah tangga Kristen di Gereja Bethel Indonesia jemaat ‘Kristus Gembala’ Juwana Pati Jawa Tengah, serta nilai korelasi pemahaman dan praktik ‘istri tunduk kepada suami’ terhadap keharmonisan rumah tangga kristen berada pada posisi kuat.
Model Penggembalaan Menurut I Petrus 5 : 2 – 3
Authors: Antjura Adolf 
Published: August 2023

Pastoral care is a very important thing in the spiritual growth of the congregation, where the role is a congregation pastor who must give his life truly according to his calling in serving God. As in 1 Peter 5:2-3, it must be done voluntarily without coercion because the service must be sincere and pure so that it can have an impact through example. This research was specifically conducted in churches to find officials' service models so that the formulation of the problem in this study: Is the service model appropriate according to 1 Peter 5:2-3? research that uses a qualitative phenomenological approach, namely by formulating and making conclusions based on data, information and facts that will be obtained through observing these phenomena, interviews and investigations, has the aim of explaining the shepherding model of a shepherd.
ABSTRAK BAHASA INDONESIA
Penggembalaan merupakan hal yang sangat penting di dalam pertumbuhan rohani jemaat, dimana yang menjadi pemerannya adalah seorang gembala sidang yang harus memberikan hidupnya sungguh-sungguh sesuai panggilannya dalam pengabdian kepada Tuhan. Seperti dalam 1 Petrus 5:2-3, harus dilakukan dengan sukarela tanpa paksaan karena pelayanan itu harus tulus dan murni sehingga bisa berdampak lewat keteladanan. Penelitian ini secara khusus dilakukan di gereja menemukan model pelayanan Pejabat sehingga rumusan masalah dalam penelitian ini: Apakah model pe;layanan sesuai menurut 1 Petrus 5:2-3? penelitian yang menggunakan pendekatan kualitatif fenomenologi, yaitu dengan merumuskan dan membuat kesimpulan-kesimpulan berdasarkan data-data, informasi dan fakta-fakta yang akan diperoleh melalui pengamatan fenomena-fenomena, wawancara dan investigasi ini, memiliki tujuan yaitu untuk menjelaskan model penggembalaan seorang gembala.
Landasan Teologis Gerakan Transformasi Gereja di Abad XXI
Authors: Maukar Fengky 
Published: August 2023

The purpose of this writing is to investigate some of the forms of the transformation movement in the Bible, and to develop a theological basis for the transformation movement, which can be used to realize real Christian transformation. This departs from the fact that the transformation movement that has taken place in the last 10 years has been filled with various forms of ministry that prioritize demonstrations of miracles and healing, prayer concerts of praise and worship, and are not directed at spiritual maturity and character building. The transformation movement is a process of changing Christian life for the better, occurring in a person personally, or a community, church, nation and state society, which covers all human life holistically. A study of the text on Romans 12:2 shows that the use of the word μεταμορφόω means transformation. Theological transformation is God's initiative to save and renew sinful humanity. The Lord Jesus came as a transformer who redeemed and saved mankind from sin. The Holy Spirit is the one who renews, and leads everyone into the truth. Ecclesiological transformation means that the church carries out the mission of the Kingdom of God in the midst of the world. Furthermore, transformation is a holistic mission that touches all aspects of human life. Transformation is an ongoing process and has an excchatological aspect.
ABSTRAK BAHASA INDONESIA
Tujuan penulisan ini adalah untuk menyelidiki sebagian bentuk-bentuk gerakan transformasi di dalam Alkitab, dan menyusun suatu landasan teologis bagi gerakan transformasi, yang dapat dipakai untuk mewujudkan transformasi Kristen sesungguhnya. Hal ini berangkat dari kenyataan gerakan transformasi yang terjadi pada 10 tahun terakhir yang dipenuhi dengan berbagai bentuk pelayanan yang mengutamakan demonstrasi mujizat dan kesembuhan, konser doa pujian dan penyembahan, dan tidak diarahkan kepada pendewasaan rohani serta pembentukan karakter. Gerakan transformasi adalah suatu proses perubahan kehidupan Kristen ke arah yang lebih baik, terjadi dalam diri seseorang secara pribadi, atau suatu komunitas, gereja, masyarakat bangsa dan negara, yang mencakup seluruh kehidupan manusia secara holistik. Studi teks pada Roma 12:2, menunjukkan bahwa kata pemakain kata μεταμορφόω memiliki arti transformasi. Transformasi secara teologis adalah inisiatif Allah untuk menyelamatkan dan memperbarui manusia yang berdosa. Tuhan Yesus datang sebagai transformator yang menebus dan menyelamatkan manusia dari dosa. Roh Kudus adalah pribadi yang memperbarui, dan memimpin setiap orang ke dalam kebenaran. Transformasi eklesiologis berarti gereja melaksanakan misi Kerajaan Allah ditengah-tengah dunia. Selanjutnya transformasi adalah misi holistik yang menyentuh seluruh aspek kehidupan manusia. Transformasi adalah proses yang terus terjadi dan memiliki aspek ekskatologi.
Metagenomics of African Empogona and Tricalysia (Rubiaceae) reveals the presence of leaf endophytes
Authors: Verstraete Brecht , Janssens Steven , De Block Petra , Asselman Pieter , Méndez Gabriela , Ly Serigne , Hamon Perla , Guyot Romain 
Published: August 2023

Background
Leaf symbiosis is a phenomenon in which host plants of Rubiaceae interact with bacterial endophytes within their leaves. To date, it has been found in around 650 species belonging to eight genera in four tribes; however, the true extent in Rubiaceae remains unknown. Our aim is to investigate the possible occurrence of leaf endophytes in the African plant genera Empogona and Tricalysia and, if present, to establish their identity.

Methods
Total DNA was extracted from the leaves of four species of the Coffeeae tribe (Empogona congesta, Tricalysia hensii, T. lasiodelphys, and T. semidecidua) and sequenced. Bacterial reads were filtered out and assembled. Phylogenetic analysis of the endophytes was used to reveal their identity and their relationship with known symbionts.

Results
All four species have non-nodulated leaf endophytes, which are identified as Caballeronia. The endophytes are distinct from each other but related to other nodulated and non-nodulated endophytes. An apparent phylogenetic or geographic pattern appears to be absent in endophytes or host plants. Caballeronia endophytes are present in the leaves of Empogona and Tricalysia, two genera not previously implicated in leaf symbiosis. This interaction is likely to be more widespread, and future discoveries are inevitable.
Vitamin D receptor-deficient keratinocytes-derived exosomal miR-4505 promotes the macrophage polarization towards the M1 phenotype
Authors: Sun Wen , Chen Jianqin , Li Jingting , She Xiaoguang , Ma Hu , Wang Shali , Liu Jing , Yuan Yuan 
Published: August 2023

Background
The vitamin D receptor (VDR) has a low level of expression in the keratinocytes of patients with psoriasis and plays a role in the development of the disease. Furthermore, the crosstalk between macrophages and psoriatic keratinocytes-derived exosomes is critical for psoriasis progression. However, the effects of VDR-deficient keratinocytes-derived exosomes (Exos-shVDR) on macrophages and their underlying mechanisms remain largely unknown.

Methods
VDR-deficient keratinocytes were constructed by infecting HaCaT cells with a VDR-targeting lentivirus, mimicking the VDR-deficient state observed in psoriatic keratinocytes. Exosomes were characterized using transmission electron microscopy, nanoparticle tracking analysis, and Western blot. The effect of Exos-shVDR on macrophage proliferation, apoptosis, and M1/M2 polarization was assessed using cell counting kit-8 assay (CCK-8), flow cytometer, real-time quantitative polymerasechain reaction (RT-qPCR), and enzyme-linked immunosorbent assay (ELISA). The mechanism underlying the effect of Exos-shVDR on macrophage function was elucidated through data mining, bioinformatics, RT-qPCR, and rescue experiments.

Results
Our results revealed that both Exos-shVDR and Exos-shNC exhibited typical exosome characteristics, including a hemispheroid shape with a concave side and particle size ranging from 50 to 100 nm. The levels of expression of VDR were significantly lower in Exos-shVDR than in Exos-shNC. Functional experiments demonstrated that Exos-shVDR significantly promoted macrophage proliferation and polarization towards the M1 phenotype while inhibiting macrophage apoptosis. Moreover, miR-4505 was highly expressed in the skin tissue of patients with psoriasis. Its overexpression significantly increased macrophage proliferation and polarization towards M1 and inhibited apoptosis. Furthermore, the effects of Exos-shVDR on macrophage function occur through miR-4505.

Conclusions
Exos-shVDR exacerbates macrophage proliferation, promotes polarization towards the M1 phenotype, and inhibits macrophage apoptosis by increasing the levels of miR-4505. These results indicate that modulation of macrophage function is a potential strategy for developing new drugs for the treatment of psoriasis.
The dosimetric impact of titanium implants in spinal SBRT using four commercial treatment planning algorithms
Authors: Liu Chieh‐Wen , Cho Young‐Bin , Magnelli Anthony , Angelov Lilyana , Balagamwala Ehsan H. , Chao Samuel T. , Xia Ping 
Published: August 2023

AbstractTo evaluate the dosimetric impact of titanium implants in spine SBRT using four dose calculation algorithms. Twenty patients with titanium implants in the spine treated with SBRT without density override (DO) were selected. The clinical plan for each patient was created in Pinnacle and subsequently imported into Eclipse (AAA and AcurosXB) and Raystation (CC) for dose evaluation with and without DO to the titanium implant. We renormalized all plans such that 90% of the tumor volume received the prescription dose and subsequently evaluated the following dose metrics: (1) the maximum dose to 0.03 cc (Dmax), dose to 99% (D99%) and 90% (D90%) of the tumor volume; (2) Dmax and volumetric metrics of the spinal cord. For the same algorithm, plans with and without DO had similar dose distributions. Differences in Dmax, D99% and D90% of the tumor were on average <2% with slightly larger variations up to 5.58% in Dmax using AcurosXB. Dmax of the spinal cord for plans calculated with DO increased but the differences were clinically insignificant for all algorithms (mean: 0.36% ± 0.7%). Comparing to the clinical plans, the relative differences for all algorithms had an average of 1.73% (−10.36%–13.21%) for the tumor metrics and −0.93% (−9.87%–10.95%) for Dmax of the spinal cord. A few cases with small tumor and spinal cord volumes, dose differences of >10% in both D99% and Dmax of the tumor, and Dmax of the spinal cord were observed. For all algorithms, the presence of titanium implants in the spine for most patients had minimal impact on dose distributions with and without DO. For the same plan calculated with different algorithms, larger differences in volumetric metrics of >10% could be observed, impacted by dose gradient at the plan normalization volume, tumor volumes, plan complexity, and partial voxel volume interpolation.
Correlation between the setting of gating window width and setup accuracy in left breast cancer radiotherapy based on deep inspiration breath hold
Authors: Yucheng Li , Yibo Shao , Wenming Zhan , Yongshi Jia , Qiang Li , Lingyun Qiu , Liu Huaxin , Zhang YingHao , jieni Ding , Weijun Chen 
Published: August 2023

AbstractPersonalized precision irradiation of patients with left‐sided breast cancer is possible by examining the setup errors of 3‐ and 4‐mm gated window widths for those treated with deep inspiration breath‐hold (DIBH) treatment. An observational study was performed via a retrospective analysis of 250 cone‐beam computed tomography (CBCT) images of 60 left‐breast cancer patients who underwent whole‐breast radiotherapy with the DIBH technique between January 2021 and 2022 at our hospital. Among them, 30 patients had a gated window width of 3 mm, while the remaining 30 had a gated window width of 4 mm; both groups received radiotherapy using DIBH technology. All patients underwent CBCT scans once a week, and the setup errors in the left‐right (x‐axis), inferior‐superior (y‐axis), and anterior‐posterior (z‐axis) directions were recorded. The clinical‐to‐planning target volume (CTV‐PTV) margins of the two gating windows were calculated using established methods. The setup error in the Y direction was 1.69 ± 1.33 mm for the 3‐mm – wide gated window and 2.42 ± 3.02 mm for the 4‐mm – wide gated window. The two groups had statistically significant differences in the overall mean setup error (Dif 0.7, 95% CI 0.15–1.31, t = 2.48, p= 0.014). The Z‐direction setup error was 2.32 ± 2.12 mm for the 3‐mm – wide gated window and 3.15 ± 3.34 mm for the 4‐mm – wide gated window. The overall mean setup error was statistically significant between the two groups (Dif 0.8, 95% CI 0.13–1.53, t= 2.34, p = 0.020). There was no significant difference in the X‐direction setup error (p > 0.05). Therefore, the CTV‐PTV margin values for a 3‐mm gated window width in the X, Y, and Z directions are 5.51, 5.15, and 7.28 mm, respectively; those for a 4‐mm gated window width in the X, Y, and Z directions are 5.52, 8.16, and 10.21 mm, respectively. The setup errors of the 3‐mm – wide gating window are smaller than those of the 4‐mm – wide gating window in the three dimensions. Therefore, when the patient's respiratory gating window width is reduced, the margin values of CTV‐PTV can be reduced to increase the distance between the PTV and the organs at risk (OARs), which ensures adequate space for the dose to decrease, resulting in lower dose exposure to the OARs (heart, lungs, etc.), thus sparing the OARs from further damage. However, some patients with poor pulmonary function or unstable breathing amplitudes must be treated with a slightly larger gating window. Therefore, this study lays a theoretical basis for personalized precision radiotherapy, which can save time and reduce manpower in the delivery of clinical treatment to a certain extent. Another potential benefit of this work is to bring awareness to the potential implications of a slightly larger gating window during treatment without considering the resulting dosimetric impact.
Aging Associates with Cilium Elongation and Dysfunction in Kidney and Pancreas
Authors: Adametz Fabian , Müller Annika , Stilgenbauer Stephan , Burkhalter Martin D. , Philipp Melanie 
Published: August 2023

AbstractCilia are best known and most studied for their manifold functions enabling proper embryonic development. Loss of cilia or dysfunction thereof results in a great variety of congenital malformations and syndromes. However, there are also cilia‐driven conditions, which manifest only later in life, such as polycystic kidney disease. Even degenerative diseases in the central nervous system have recently been linked to alterations in cilia biology. Surprisingly though, there is very little knowledge regarding cilia in normally aged organisms absent any disease. Here, it is provided evidence that cilia in naturally aged mice are considerably elongated in the kidney and pancreas, respectively. Moreover, such altered cilia appear to have become dysfunctional as indicated by changes in cellular signaling.
Engineering of Uniform Epidermal Layers via Sacrificial Gelatin Bioink‐Assisted 3D Extrusion Bioprinting of Skin
Authors: Ahn Minjun , Cho Won‐Woo , Lee Hanju , Park Wonbin , Lee Seok‐Hyeon , Back Jae Woo , Gao Qiqi , Gao Ge , Cho Dong‐Woo , Kim Byoung Soo 
Published: August 2023

AbstractTo reconstruct an ideal full‐thickness skin model, basal keratinocytes must be distributed as a confluent monolayer on the dermis. However, the currently available extrusion bioprinting method for the skin is limited when producing an air‐exposed cellular monolayer because the cells are encapsulated within a bioink. This is the first study to use sacrificial gelatin‐assisted extrusion bioprinting to reproduce a uniform and stratified epidermal layer. Experimental analyses of the rheological properties, printability, cell viability, and initial keratinocyte adhesion shows that the optimal gelatin bioink concentration is 4 wt.%. The appropriate thickness of the bioprinted gelatin structure for achieving a confluent keratinocyte layer is determined to be 400 µm. The suggested strategy generates a uniform keratinocyte monolayer with tight junctions throughout the central and peripheral regions, whereas manual seeding generates non‐uniform cellular aggregates and vacancies. These results influence gene expression, exhibiting a propensity for epidermal differentiation. Finally, the gelatin‐assisted keratinocytes are bioprinted onto a dermis composed of gelatin methacryloyl and dermis‐derived decellularized extracellular matrix to establish a full‐thickness skin model. Thus, this strategy leads to significant improvements in epidermal differentiation/stratification. The findings demonstrate that the gelatin‐assisted approach is advantageous for recreating reliable full‐thickness skin models with significant consistency for mass production.
A Biomimetic Nanoplatform with Improved Inflammatory Targeting Behavior for ROS Scavenging‐Based Treatment of Ulcerative Colitis
Authors: Wang Xiaofen , Jiao Menglu , Tian Fangzhen , Lu Xuan , Xiong Huihuang , Liu Fan , Wan Yiqun , Zhang Xiaodong , Wan Hao 
Published: August 2023

AbstractUlcerative colitis (UC), a refractory disease, has become a global problem. Herein, a biomimetic nanoplatform (AU‐LIP‐CM) comprising Au cluster enzymes (AU)‐loaded liposomes (AU‐LIP) camouflaged with the fusion membrane (CM) consisting of neutrophil (NC) and red blood cell (RBC) membrane is designed for the treatment of UC. Briefly, revealed by second near‐infrared (NIR‐II) imaging through collection of fluorescence emitting >1200 nm from AU, the improved inflammatory targeting behavior contributed by CM cloaking, which inherits abilities of inflammatory targeting and immune escape from NC and RBC, respectively, promotes specific accumulation of AU within inflammatory intestines with up to ≈11.5 times higher than that of bare AU. Afterward, AU possessing superoxide dismutase‐ and catalase‐like activities realizes high‐efficiency scavenging of reactive oxygen species (ROS), leading to repair of intestinal barriers, regulation of the immune system, and modulation of gut microbiota, which surpass first‐line UC drug. In addition, study of underlying therapeutic mechanism demonstrated that the treatment with AU‐LIP‐CM can alter the gene signature associated with response to ROS for UC mice to a profile similar to that of healthy mice, deciphering related signal pathways. The strategy developed here provides insights of learning from properties of natural bio‐substances to empower biomimetic nanoplatform to confront diseases.
Natural Wood‐Derived Macroporous Cellulose for Highly Efficient and Ultrafast Elimination of Double‐Stranded RNA from In Vitro‐Transcribed mRNA
Authors: Yuan Xiushuang , Wu Zhanfeng , Guo Jing , Luo Dengwang , Li Tianyao , Cao Qinghao , Ren Xiangyu , Fang Han , Xu Dawei , Cao Yuhong 
Published: August 2023

AbstractDouble‐stranded RNA (dsRNA) is a major impurity that can induce innate immune responses and cause adverse drug reactions. Removing dsRNA is an essential and non‐trivial process in manufacturing mRNA. Current methods for dsRNA elimination use either high‐performance liquid chromatography or microcrystalline cellulose, rendering the process complex, expensive, toxic, and/or time‐consuming. This study introduces a highly efficient and ultrafast method for dsRNA elimination using natural wood‐derived macroporous cellulose (WMC). With a naturally formed large total pore area and low tortuosity, WMC removes up to 98% dsRNA within 5 min. This significantly shortens the time for mRNA purification and improves purification efficiency. WMC can also be filled into chromatographic columns of different sizes and integrates with fast‐protein liquid chromatography for large‐scale mRNA purification to meet the requirements of mRNA manufacture. This study further shows that WMC purification improves the enhanced green fluorescent protein mRNA expression efficiency by over 28% and significantly reduces cytokine secretion and innate immune responses in the cells. Successfully applying WMC provides an ultrafast and efficient platform for mRNA purification, enabling large‐scale production with significant cost reduction.
Electro‐Responsive Breathing Transition of Conductive Hydrogel for Broadband Kinetic Energy Harvesting
Authors: Li Zhou , Yun Huiru , Yan Yuke , Yuan Man , Zhao Yang , Zhao Fei 
Published: August 2023

AbstractReclaiming kinetic energy from vibrating machines holds great promise for sustainable energy harvesting technologies. Nevertheless, the impulsive current induced by vibrations is incompatible with conventional energy storage devices. The energy‐management system necessitates novel designs of soft materials for lightweight, miniaturized, and integrated high‐frequency electrochemical devices. Here, this work develops a conductive hydrogel with an electro‐responsive polymeric network. The electro‐responsive breathing transition of the crosslinking points facilitates the expeditious formation of a localized electrolyte layer. This layer features an exceedingly high local charge density, surpassing that of a saturated electrolyte solution by an order of magnitude, and thus enabling rapid charge transport under the influence of an applied voltage. The micro‐capacitor based on the gel exhibits record‐high capacitance of ≈2 mF cm−2 when the frequency of energy input reaches up to 104 Hz. This work also demonstrates a prototype battery charger that harvests energy from a running car engine. This study presents a feasible strategy for waste energy recycling using integrated electrochemical devices, opening a new avenue for ambient energy management.