bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA GABPB1‑IT1 inhibits the tumorigenesis of renal cancer via the miR‐21/PTEN axis
Authors: Tan Chao , Du Huasheng , Wang Yang , Zhao Jianjun , Cheng Xiaolong , Lan Haihe 
Published: February 2023

AbstractLong noncoding RNA (lncRNA) (GABPB1‑IT1) has been reported to be downregulated in lung cancer, while its expression and function in other cancers are unknown. In this study, the expression levels of GABPB1‑IT1 in tissue samples from 62 ccRCC patients were measured by performing RT‐qPCR. Potential base pairing formed between GABPB1‑IT1 and miR‐21 was explored using the online program IntaRNA 2.0 and further confirmed by Dual‐luciferase activity assay and RNA pulldown assay. The role of GABPB1‑IT1 and miR‐21 in regulating the expression of PTEN was evaluated by RT‐qPCR and Western blot. The role of GABPB1‑IT1, miR‐21, and PTEN in regulating the proliferation of Caki‐2 cells was explored by CCK‐8 assay. It was observed that GABPB1‑IT1 was downregulated in ccRCC and predicted poor survival. GABPB1‑IT1 directly interacted with miR‐21, while it did not regulate the expression of each other. Moreover, upregulation of PTEN, which is a target of miR‐21, was observed in ccRCC cells with overexpression of GABPB1‑IT1. Overexpression of GABPB1‑IT1 and PTEN decreased the proliferation rates of ccRCC cells. In addition, overexpression of GABPB1‑IT1 reduced the enhancing effects of miR‐21 on cell proliferation. Therefore, GABPB1‑IT1 may upregulate PTEN by sponging miR‐21 in ccRCC to inhibit cancer cell proliferation. Our study characterized a novel GABPB1‑IT1/miR‐21/PTEN axis in ccRCC.
Initial perception of the mentoring role and related mentors' approach of autonomy support or control in formal youth mentoring relationships
Authors: Javornicky Brumovska Tereza , Seidlova Malkova Gabriela 
Published: February 2023

AbstractMentors' initial perceptions of the mentoring role in formal youth mentoring bonds; and the subsequent characteristics of autonomy support or autonomy control in mentoring interactions developed by mentors after 5 months of mentoring experience are discussed in this paper. The data is drawn from a longitudinal phenomenological study conducted in the Czech mentoring scheme between 2010 and 2017. In‐depth semi‐structured interviews were collected with 10 mentoring matches over 1 year of mentoring involvement. The results of Interpretive phenomenological analysis showed differences in mentors' initial perceptions of the role, and related autonomy‐supportive or autonomy‐controlling characteristics in mentors' approach. The benefits and risks of resulting autonomy support or control in mentoring interactions are discussed. The results argue for the theoretical conceptualisation of a child‐centred perspective in youth mentoring that aims at mentees' support of autonomy, active agency and empowement, thus arguing for further in‐depth exploration of natural mentoring principles in child‐centred perspective, supporting approaches such as youth‐initiated mentoring, and broadening the discussion on good evidence‐based mentoring practice in the EU context.
Legacy phosphorus in Alabama Hartsells soil after long‐term amendment with broiler litter
Authors: Waldrip Heidi M. , Campbell Terra N. , Koziel Jacek A. , Watts Dexter B. , Torbert Henry Allen 
Published: February 2023

AbstractNumerous studies have investigated effects of long‐term manure application on total phosphorus (P) and inorganic P (Pi), but few have evaluated soil organic P (Po). Little is known about crop management effects on Po in soils with varying minerology. In this study, sequential fractionation was used to characterize specific P forms after 25 years of broiler litter (BL) or ammonium nitrate (Con) applications to an Alabama Hartsells soil. Crops (corn [Zea mays L.], soybean [Glycine Willd.], and corn or soybean with a wheat [Triticum aestivum L.] cover crop) were under conventional tillage (CT) or no‐tillage (NT). Regardless of crop, tillage, or fertilizer type, the proportion of extractable Pi was relatively stable at 21%–49% at 0–5 cm and 25%–45% at 5–10 cm. Extractable Pi ranged from 0.69 to 2.4 mg g−1. BL increased total extractable Pi (p ≤ 0.001) at 0–5 cm and 5–10 cm. Total extractable P was influenced at 0–5 cm (p ≤ 0.006) by both tillage and fertilization type, but not at 5–10 cm or at either depth in soybean plots. Long‐term BL application increased total extractable soil P at 0–5 cm. In corn systems, CT did not reduce P loading to topsoil or result in P leaching to lower soil depths, compared to NT. Soybean and soybean–wheat reduced P loading in BL plots, compared to corn and corn–wheat. Soil Po was classed in the order of monoesters > phytate and polyphosphates, where most was extractable with NaOH. BL increased extractable Po in all fractions. Care should be taken when applying BL to highly weathered soils to avoid legacy Po accumulation. Soybean rotations and cover crops could help remediate P‐laden soils after repeated BL application.
Metal/catalyst/alkyne/alkene‐free one‐pot synthesis of indolizines from 2‐(pyridin‐2‐yl)acetate, DMF‐DMA and α‐halomethylenes
Authors: Jalani Hitesh B. , Lee Doha , Lee Ju‐Young , Jeong Jin‐Hyun 
Published: February 2023

AbstractWe have described a simple and robust one‐pot synthesis of 1,3‐disubstitued‐indolizines from 2‐(pyridin‐2‐yl)acetate, DMF‐DMA and α‐halomethylenes without using metal/catalyst/alkyne/alkene. This protocol provides variety of indolizine analogs via in‐situ formed 3‐(dimethylamino)‐2‐(pyridin‐2‐yl)acrylate intermediate, undergoes N‐alkylation followed by Michael addition/cyclization. The gram scale synthesis of indolizine developed by this protocol does not require chromatographic purification. The post synthesis application of alkyne bearing indolizine provided triazole and a beta‐ketoester containing indolizine provided indolizine‐tethered pyrimidine heterocyclic system.
Acceptability and retention of the key population‐led HIV treatment service for men who have sex with men and transgender women living with HIV in Thailand
Authors: Lujintanon Sita , Amatavete Sorawit , Leenasirimakul Prattana , Meechure Jantana , Noopetch Preudtipong , Sangtong Supakarn , Sittikarn Satayu , Phoopisutthisak Poonnanat , Seekaew Pich , Mills Stephen , Phanuphak Praphan , Ramautarsing Reshmie A. , Phanuphak Nittaya 
Published: February 2023

AbstractIntroductionIn Thailand, where the HIV epidemic is concentrated among key populations (KPs), particularly men who have sex with men (MSM) and transgender women (TGW), an HIV service delivery model tailored to KPs was piloted. This study evaluated the acceptability and retention of clients who accepted and declined the KP‐led HIV treatment service.MethodsA retrospective cohort study was conducted using secondary data from three community‐based organizations (CBOs) and three hospitals in Thailand. KP lay providers were trained to lead HIV treatment service in which MSM and TGW living with HIV received counselling and a 3‐month antiretroviral therapy (ART) supply at CBOs. Thai MSM and TGW who were at least 18 years, on ART for at least 6–12 months, without co‐morbidities/co‐infections, and virally suppressed were eligible and offered the service. Those who declined received ART via other service models offered by the hospitals and served as a comparison group.ResultsOf 220 clients screened between February 2019 and February 2020, 72% (159/220) were eligible of which 146 were MSM and 13 were TGW. Overall, 45% (72/159) accepted the KP‐led service. Of those who declined, 98% (85/87) preferred to see the physician at the hospital. After 12 months of follow‐up, among those accepted, 57% were in care at the CBO, 32% were referred back to and in care in other service models offered by the hospital, 10% were successfully transferred out to other hospital and 1% were lost to follow‐up (LTFU); among those declined, 92% were in care in any service models offered by the hospital, 5% were successfully transferred out to other hospital, 2% were LTFU and 1% died (p‐value<0.001).ConclusionsDespite moderate acceptability and retention in care at the CBO among the clients accepting the KP‐led service, almost all clients were engaged in care overall. Multiple service models that meet the preferences and needs of KPs living with HIV should be available to optimize engagement in care.
Gender‐focused or gender mainstreaming programmes? The gender dimension of international aid
Authors: Su Fang‐Ying , Yang Wan‐Ying 
Published: February 2023

AbstractAid in support of gender equality and women's empowerment has gathered significant momentum over the past decade and has also sparked a renewed debate over the effectiveness of foreign aid. Is gender aid a panacea that will help the recipient countries confront the challenges of the gender inequality deeply ingrained in their societies, or is it just a placebo pretending to be effective? Is there any difference between gender‐focused aid and gender mainstreaming aid in terms of aid effectiveness? To answer these key questions, this article examines the impact of gender aid on gender inequality using a sample of 116 recipient countries that covers the period from 2010 to 2019. By adopting a hybrid method that incorporates fixed‐ and random‐effects models, we obtain results indicating that increasing amounts of gender mainstreaming aid over time successfully reduce gender inequality and close the gender gap. In stark contrast, gender‐focused aid lacks the statistical significance needed to prove its potency. In addition to confirming gender aid's effectiveness, this article provides further policy implications regarding the gender mainstreaming strategy in the field of international development, particularly when considered in the context of the 2030 Agenda for Sustainable Development.
Cross‐reactivity of SARS‐CoV‐2 with other pathogens, especially dengue virus: A historical perspective
Authors: Dutta Debrupa , Ghosh Anisa , Dutta Chiroshri , Sukla Soumi , Biswas Subhajit 
Published: February 2023

AbstractDengue is a vector‐borne viral disease caused by a Flavivirus whereas the COVID‐19 pandemic was caused by a highly contagious virus, SARS‐CoV‐2 belonging to the family Coronaviridae. However, COVID‐19 severity was observably less in dengue‐endemic countries and vice versa especially during the active years of the pandemic (2019–2021). We observed that dengue virus (DENV) antibodies (Abs) could cross‐react with SARS‐CoV‐2 spike antigen. This resulted in SARS‐CoV‐2 false positivity by rapid Ab test kits. DENV Abs binding to SARS‐CoV‐2 receptor‐binding domain (and the reverse scenario), as revealed by docking studies further validated DENV and SARS‐CoV‐2 cross‐reactivity. Finally, SARS‐CoV‐2 Abs were found to cross‐neutralize DENV1 and DENV2 in virus neutralization test (VNT). Abs to other pathogens like Plasmodium were also cross‐reactive but non‐neutralizing for SARS‐CoV‐2. Here, we analyze the existing data on SARS‐CoV‐2 cross‐reactivity with other pathogens, especially dengue to assess its impact on health (cross‐protection?) and differential sero‐diagnosis/surveillance.
Immunogenicity of novel DNA vaccines encoding receptor‐binding domain (RBD) dimer‐Fc fusing antigens derived from different SARS‐CoV‐2 variants of concern
Authors: Zhang Ting , Wang Zhirong , Yang Jiaojiao , Xu Xuemei 
Published: February 2023

AbstractThe continuously emerging of severe acute respiratory syndrome coronavirus‐2 variants of concern (VOCs) led to a decline in effectiveness of the first‐generation vaccines. Therefore, optimized vaccines and vaccination strategies, which show advantages in protecting against VOCs, are urgently needed. Here we constructed an optimized DNA vaccine plasmid containing built‐in CpG adjuvant, and designed vaccine candidates encoding five forms of antigens derived from Wuhan‐Hu‐1. The results showed that plasmid with receptor binding domain (RBD) dimer‐Fc fusing antigen (2RBD‐Fc) induced the highest level of RBD‐specific IgG and neutralizing antibodies in mice. Then 2dRBD‐Fc and 2omRBD‐Fc vaccines, respectively derived from delta and omicron VOCs, were constructed. The 2dRBD‐Fc induced potent humoral and cellular immune responses, while the immunogenicity of 2omRBD‐Fc was low. We also observed that sequential immunization with 2RBD‐Fc, 2dRBD‐Fc and 2omRBD‐Fc effectively elicited neutralizing antibodies against each immunized strain, and RBD‐specific T cell responses. To be noted, the Wuhan‐Hu‐1, delta and omicron neutralizing antibody titers induced by sequential immunization were comparable to that induced by repetitive immunization with 2RBD‐Fc, 2dRBD‐Fc or 2omRBD‐Fc respectively. The results suggest that sequential immunization with DNA vaccines encoding potent antigens derived from different VOCs, may be a promising strategy to elicit immune responses against multiple variants.
Whole‐genome single molecule real‐time sequencing of SARS‐CoV‐2 Omicron
Authors: Nicot Florence , Trémeaux Pauline , Latour Justine , Carcenac Romain , Demmou Sofia , Jeanne Nicolas , Ranger Noémie , De Smet Clémentine , Raymond Stéphanie , Dimeglio Chloé , Izopet Jacques 
Published: February 2023

AbstractNew variants and genetic mutations of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) genome can only be identified using accurate sequencing methods. Single molecule real‐time (SMRT) sequencing has been used to characterize Alpha and Delta variants, but not Omicron variants harboring numerous mutations in the SARS‐CoV‐2 genome. This study assesses the performance of a target capture SMRT sequencing protocol for whole genome sequencing (WGS) of SARS‐CoV‐2 Omicron variants and compared it to that of an amplicon SMRT sequencing protocol optimized for Omicron variants. The failure rate of the target capture protocol (6%) was lower than that of the amplicon protocol (34%, p < 0.001) on our data set, and the median genome coverage with the target capture protocol (98.6% [interquartile range (IQR): 86–99.4]) was greater than that with the amplicon protocol (76.6% [IQR: 66–89.6], [p < 0.001]). The percentages of samples with >95% whole genome coverage were 64% with the target capture protocol and 19% with the amplicon protocol (p < 0.05). The clades of 96 samples determined with both protocols were 93% concordant and the lineages of 59 samples were 100% concordant. Thus, target capture SMRT sequencing appears to be an efficient method for WGS, genotyping and detecting mutations of SARS‐CoV‐2 Omicron variants.
Projected changes in synoptic circulations over Europe and their implications for summer precipitation: A CMIP6 perspective
Authors: Herrera‐Lormendez Pedro , John Amal , Douville Hervé , Matschullat Jörg 
Published: February 2023

AbstractProjected changes in summer precipitation deficits partly depend on alterations in synoptic circulations. Here, the automated Jenkinson–Collison classification is used to assess the ability of 21 global climate models (GCMs) to capture the frequency of recurring circulation types (CTs) and their implications for European daily precipitation amounts in summer (JJA). The ability of the GCMs to reproduce the observed present‐day climate features is evaluated first. Most GCMs capture the observed links between the mean CTs directional flow characteristics and the occurrence of dry days and related dry months. The most robust relationships are found for anticyclonic and easterly CTs which are generally associated with higher‐than‐average occurrences of dry conditions. Future changes in summer CTs' frequencies are estimated in the high‐emission SSP5‐8.5 scenario for the sake of a high signal‐to‐noise ratio. Our results reveal consistent changes, mainly in the zonal CTs. A robust decrease in frequency of the westerlies and an increase in the frequency of easterly CTs favour more continental, dry and warm air masses over central Europe. These dynamical changes are shown to enhance the projected summer drying over central and southern Europe.
Dynamic downscaling of climate simulations and projected changes in tropical South America using RegCM4.7
Authors: da Silva Maria Leidinice , de Oliveira Cristiano Prestrelo , Santos e Silva Cláudio Moisés , de Araújo João Medeiros 
Published: February 2023

AbstractHigh‐resolution (dx = 25 km) simulations of the regional climate model RegCM4.7 coupled with the Community Land Model version 4.5 (CLM4.5) under low (RCP2.6) and high (RCP8.5) emissions scenarios, which interact with the Fifth Assessment Report of the Intergovernmental Panel on Climate Change (AR5‐IPCC), were carried out over tropical South America (TSA). These simulations were produced through boundary conditions from simulations driven by the general circulation model HadGEM2‐ES. With the goal of verifying the added value (AV) of RegCM4.7, reproducing in an adequate and coherent way the regional aspects of the historical period (1986–2005), as well as evaluating the regional aspects simulated by the model in the scope of the change projections for the far‐future (2080–2099). For this study, the climate in the TSA was characterized based on the variables of precipitation and near‐surface air temperature. For the evaluation of the spatial–temporal representation, frequency and distribution of the regional and global simulation, the high‐resolution observational dataset of the Climate Research Unit version ts4.02 (CRU) was used. Although some differences and biases still persist, RegCM4.7 presents AV in the spatial representation of precipitation and temperature over the northeast region of Brazil and part of the Andes, especially in winter. However, it does not adequately represent precipitation over the Amazon Basin, especially in summer. Results for future projections indicate that the more refined simulation of RegCM4.7 improves the projected change patterns of the coarser resolution simulation of HadGEM2‐ES and even modifies the precipitation signal in some cases. Both models project increase temperature with greater magnitude for RCP8.5. RegCM4.7 presents a much more refined and realistic spatial distribution. HadGEM2‐ES simulates the major aspects of climate enough to consider forcing RegCM4.7 to generate simulations with better performance and more realistic projections.
World Reference Base for Soil Resources—Its fourth edition and its history
Authors: Schad Peter 
Published: February 2023

AbstractThe fourth edition of the international soil classification system World Reference Base for Soil Resources (WRB) was released in 2022. It maintains the 32 Reference Soil Groups at the first classification level. Most qualifiers (second level) and most diagnostic horizons, properties and materials were maintained but some were abolished and new ones introduced. The main part of the fourth edition is followed by six annexes, most of them are new. For the first time, the WRB has a Field Guide (Annex 1) to facilitate field survey and to assure that all field characteristics required in the classification are reported. The fourth edition also provides designations for horizons and layers (Annex 3), which was not the case in the second and the third edition. The wordings of the definitions were harmonized, and the same features are worded in the same way throughout the text (including the annexes). Ambiguities have been corrected and many definitions written in a more concise and a more didactical way. The WRB has a long history. Four editions have been published: 1998, 2006 (with update 2007), 2014 (with update 2015) and 2022. Editor is the Working Group WRB of the International Union of Soil Sciences. The WRB followed the Legend and the Revised Legend of the Soil Map of the World. This map was edited by FAO (Food and Agriculture Organization of the United Nations) and UNESCO, and the system is known as the FAO Soil Classification System. In addition, WRB incorporated ideas from the former Working Group International Reference Base for Soil Classification that existed from 1982 to 1994.
Matrine regulates Th1/Th2 inflammatory responses by inhibiting the Hsp90/NF‐κB signaling axis to alleviate atopic dermatitis
Authors: Huang Pan , Hu Fan , Yang Zhi‐Bo , Pan Yi , Zhou Rong , Yan Yi‐Ning , Wang Hai‐Zhen , Wang Chang 
Published: February 2023

AbstractAtopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine Sophora flavescens, and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and its underlying mechanism. An AD mouse model and inflamed human epidermal keratinocyte cells (HaCaT) cells were established. Histopathological aspects were examined using hematoxylin and eosin staining, toluidine blue staining, and immunohistochemistry. The mRNA and protein expressions were assessed using quantitative real‐time polymerase chain reaction and Western blot, respectively. The secretions of cytokines and chemokines were examined by enzyme‐linked immunosorbent assay. Flow cytometry was carried out to analyze the proportions of T‐helper (Th) 1 and Th2 cells. Herein, our results displayed that matrine diminished AD symptoms and decreased heat shock protein 90 (Hsp90) expression. Matrine decreased the Th2 cytokine levels in the ear tissues and serum, and it also significantly repressed inflammatory cytokines (thymus activation regulated chemokine and interleukin‐6) secretions by repressing the Hsp90/NF‐κB signaling axis in inflamed HaCaT cells. Furthermore, matrine inhibited Th2 differentiation of CD4+ T cells when co‐cultured with inflamed HaCaT cells. Matrine can regulate the Th1/Th2 inflammatory response by inhibiting the Hsp90/NF‐κB signaling axis to alleviate AD. Therefore, it may be a candidate for AD treatment.
Cochlear Implantation for Unilateral and Asymmetric Hearing Loss: Long‐Term Subjective Benefit
Authors: Thompson Nicholas J. , Lopez Erin M. , Dillon Margaret T. , Rooth Meredith A. , Richter Margaret E. , Pillsbury Harold C. , Brown Kevin D. 
Published: February 2023

ObjectivesAssess the long‐term patterns of perceived tinnitus severity and subjective benefit for adult cochlear implant (CI) users with asymmetric or unilateral hearing loss (AHL or UHL).MethodsForty adults underwent cochlear implantation as part of a prospective clinical trial assessing the outcomes of CI use in cases of AHL (n = 20) and UHL (n = 20). Subjective measures included the Tinnitus Handicap Inventory (THI), the Speech, Spatial, & Qualities of Hearing Scale (SSQ), and the Abbreviated Profile of Hearing Aid Benefit (APHAB). Responses were obtained preoperatively and at routine intervals out to 5 years post‐activation.ResultsFor subjective benefit, participants with AHL and UHL reported a significant improvement as compared to preoperative abilities, which was maintained with long‐term CI use. For perceived tinnitus severity, participants with AHL and UHL reported a significant reduction with CI use as compared to preoperative perceptions. The perceived tinnitus severity significantly differed for the AHL and UHL cohorts over time. This pattern of results is likely influenced by the worse perceived severity levels for the UHL cohort preoperatively and the fluctuating perceived severity levels for some participants in the AHL cohort post‐activation.ConclusionAdults with AHL and UHL report an early, significant reduction in perceived tinnitus severity and improvement in quality of life with CI use that is generally maintained with long‐term device use. Questionnaires such as the THI, SSQ, and APHAB may contribute to a more holistic assessment of the benefits of cochlear implantation in this population.Level of Evidence2 Laryngoscope, 133:2792–2797, 2023