bluuebunny/crossref_metadata_2025_split huggingface dataset:
An in vitro study into three different PRF preparations for osteogenesis potential
Authors: Kosmidis Kostantinos , Ehsan Karishma , Pitzurra Luciano , Loos Bruno , Jansen Ineke 
Published: March 2023

AbstractObjectiveTo investigate the effect of Advanced Platelet‐Rich Fibrin (A‐PRF+), Leukocyte Platelet‐Rich Fibrin (L‐PRF), and injectable Platelet‐Rich Fibrin (i‐PRF) on osteogenesis of a human osteoblast‐like cell line in vitro.BackgroundDifferent PRF protocols are used in clinical dentistry in the last years. Recent literature documented the positive impact of PRF derivatives in vivo and in vitro, on different types of cells. However, hardly any literature comparing the new protocols for PRF (the A‐PRF+ and i‐PRF) with the original protocol of PRF (L‐PRF) is present for osteoblast‐like cells.Materials and MethodsA‐PRF+, L‐PRF, and i‐PRF were prepared from six male donors and pre‐cultured with 10 mL culture medium for 6 days. 5 x 103 cells/ml osteoblasts from the osteoblast cell line (U2OS) were seeded and cultured either with conditioned medium derived from the different PRF conditions or with regular culture medium. At five different time points (0, 7, 14, 21, 28 days), the osteogenic capacity of the cells was assessed with Alizarin Red S to visualize mineralization. Also in these cells, the calcium concentration and alkaline phosphatase activity were investigated. Using qPCR, the expression of alkaline phosphatase, osteocalcin, osteonectin, ICAM‐1, RUNX‐2, and collagen 1a was assessed.ResultsIn osteoblast‐like cells cultured with conditioned medium, the A‐PRF+ conditioned medium induced more mineralization and calcium production after 28 days of culturing compared with the control (p < .05). No significant differences were found in the extent of cell proliferation between the different conditions. RUNX‐2 and osteonectin mRNA expression in the cells were lower in all PRF‐stimulated cultures compared with control at different time points. The i‐PRF‐conditioned medium induced more ALP activity (p < .05) compared with control and osteoblasts‐like cells differentiated more compared with osteoblasts cultured with L‐PRF.ConclusionsThe three PRF preparations seem to have the capacity to increase the osteogenic potential of osteoblast‐like cells. A‐PRF+ seems to have the highest potential for mineralization, while i‐PRF seems to have the potential to enhance early cell differentiation.
Sleep electroencephalography biomarkers of cognition in obstructive sleep apnea
Authors: Gu Yusing , Gagnon Jean‐François , Kaminska Marta 
Published: March 2023

SummaryObstructive sleep apnea has been associated with cognitive impairment and may be linked to disorders of cognitive function. These associations may be a result of intermittent hypoxaemia, sleep fragmentation and changes in sleep microstructure in obstructive sleep apnea. Current clinical metrics of obstructive sleep apnea, such as the apnea–hypopnea index, are poor predictors of cognitive outcomes in obstructive sleep apnea. Sleep microstructure features, which can be identified on sleep electroencephalography of traditional overnight polysomnography, are increasingly being characterized in obstructive sleep apnea and may better predict cognitive outcomes. Here, we summarize the literature on several major sleep electroencephalography features (slow‐wave activity, sleep spindles, K‐complexes, cyclic alternating patterns, rapid eye movement sleep quantitative electroencephalography, odds ratio product) identified in obstructive sleep apnea. We will review the associations between these sleep electroencephalography features and cognition in obstructive sleep apnea, and examine how treatment of obstructive sleep apnea affects these associations. Lastly, evolving technologies in sleep electroencephalography analyses will also be discussed (e.g. high‐density electroencephalography, machine learning) as potential predictors of cognitive function in obstructive sleep apnea.
Nitrogen and phosphorus budgets of a polyculture system containing Penaeus vannamei, Siganus guttatus, and Selenotoca multifasciata
Authors: Wang Jinjiao , Luo Dongshui , Liu Jianyong 
Published: March 2023

AbstractThe study was designed and conducted to optimize the structure of polyculture for Penaeus vannamei, Siganus guttatus (omnivorous fish), and Selenotoca multifasciata (carnivorous fish), and to compare the changes in yield, survival, and utilization rates of nitrogen (N) and phosphorus (P). Five treatments were performed as follows: Control (C) (0.0719 kg/m3, 0, 0), PSS1(0.0719 kg/m3, 0.0018 kg/m3, 0.0008 kg/m3), PSS2 (0.0719 kg/m3, 0.0033 kg/m3, 0.0016 kg/m3), PSS3 (0.0719 kg/m3, 0.0047 kg/m3, 0.0023 kg/m3), and PSS4 (0.0719 kg/m3, 0.0064 kg/m3, 0.0031 kg/m3) (with P:Penaeus vannamei, S:Siganus guttatus, and S: Selenotoca multifasciata). The yield and survival of P. vannamei in different treatments were significantly different (P<0.05), with the highest yields being P. vannamei (718.75 kg/hm3), S. guttatus (32.97 kg/hm3), and S. multifasciata (15.63 kg/hm3) for treatment PSS2. The highest survival of P. vannamei was 89.4 ± 1.6% for treatment PSS2, while the highest N and P utilization rates were 27.7% and 37.5% for treatment PSS3. The polyculture mode when using a suitable density can improve production and survival of P. vannamei, as well as the utilization rates of N and P.
Genome‐wide phylogeography reveals cryptic speciation in the circumglobal planktonic calcifier Limacina bulimoides
Authors: Choo L. Q. , Spagliardi G. , Malinsky M. , Choquet M. , Goetze E. , Hoarau G. , Peijnenburg K. T. C. A. 
Published: March 2023

AbstractLittle is known about when and how planktonic species arise and persist in the open ocean without apparent dispersal barriers. Pteropods are planktonic snails with thin shells susceptible to dissolution that are used as bio‐indicators of ocean acidification. However, distinct evolutionary units respond to acidification differently, and defining species boundaries is therefore crucial for predicting the impact of changing ocean conditions. In this global population genomic study of the shelled pteropod Limacina bulimoides, we combined genetic (759,000 single nucleotide polymorphisms) and morphometric data from 161 individuals, revealing three major genetic lineages (FST = 0.29–0.41): an “Atlantic lineage” sampled across the Atlantic, an “Indo‐Pacific lineage” sampled in the North Pacific and Indian Ocean, and a “Pacific lineage” sampled in the North and South Pacific. A time‐calibrated phylogeny suggests that the lineages diverged about 1 million years ago, with estimated effective population size remaining high (~10 million) throughout Pleistocene glacial cycles. We do not observe any signatures of recent hybridization, even in areas of sympatry in the North Pacific. While the lineages are reproductively isolated, they are morphologically cryptic, with overlapping shell shape and shell colour distributions. Despite showing that the circumglobal L. bulimoides consists of multiple species with smaller ranges than initially thought, we found that these pteropods still possess high levels of genetic variability. Our study adds to the growing evidence that speciation is often overlooked in the open ocean, and suggests the presence of distinct biological species within many other currently defined circumglobal planktonic species.
Social and paternal female choice for male MHC genes in golden snub‐nosed monkeys (Rhinopithecus roxellana)
Authors: Zhang Pei , Zhang Bingyi , Dunn Derek W. , Song Xiaoyue , Huang Kang , Dong Shixuan , Niu Fei , Ying Meijing , Zhang Yingying , Shang Yixin , Pan Ruliang , Li Baoguo 
Published: March 2023

AbstractGenes of the major histocompatibility complex (MHC) play important roles in vertebrate immunocompetence. MHC genes thus offer females indirect benefits to mate choice through the production of offspring of an optimal MHC genotype. Females may choose males with specific MHC haplotypes, dissimilar MHC genotypes, MHC heterozygous males or MHC‐diverse males. We tested these four alternatives for both female social and paternal choice in wild golden snub‐nosed monkeys (Rhinopithecus roxellana) by examining overall genetic variability (via microsatellites) and four MHC‐genes (DRB1, DRB2, DQA1 and DQB1). Monte Carlo randomization tests showed that MHC dissimilarity was favoured for social choice (males to which females were socially affiliated) and intermediate MHC dissimilarity was favoured in paternal choice (fathers of offspring). No evidence of inbreeding avoidance was found for either social or paternal mates. We found that MHC heterozygotes, higher microsatellite multilocus heterozygosity and higher microsatellites diversity were favoured for social mates, and higher microsatellite diversity was favoured for paternal mates. Independent of male age, we found that the formation of male–female social pairings is significantly predicted by compatibility based on the sharing of MHC haplotypes. However, we found no evidence of independent genetic effects on the duration of male–female social pairings, male social status (achieving OMU leader male status or not), the number of females with which individual leader males paired, the likelihood of potential male–female pairings producing offspring, or whether males fathered offspring or not. Overall, our findings suggest different genetic factors are involved in social and paternal choice in R. roxellana.
Evolution of the jasmonate ligands and their biosynthetic pathways
Authors: Chini Andrea , Monte Isabel , Zamarreño Angel M. , García‐Mina José M. , Solano Roberto 
Published: March 2023

Summary
Different plant species employ different jasmonates to activate a conserved signalling pathway in land plants, where (+)‐7‐iso‐JA‐Ile (JA‐Ile) is the ligand for the COI1/JAZ receptor in angiosperms and dn‐cis‐OPDA, dn‐iso‐OPDA and Δ4‐dn‐iso‐OPDA act as ligands in Marchantia polymorpha. In addition, some jasmonates play a COI1‐independent role.
To understand the distribution of bioactive jasmonates in the green lineage and how their biosynthetic pathways evolved, we performed phylogenetic analyses and systematic jasmonates profiling in representative species from different lineages.
We found that both OPDA and dn‐OPDA are ubiquitous in all tested land plants and present also in charophyte algae, underscoring their importance as ancestral signalling molecules. By contrast, JA‐Ile biosynthesis emerged within lycophytes coincident with the evolutionary appearance of JAR1 function.
We identified that the OPR3‐independent JA biosynthesis pathway is ancient and predates the evolutionary appearance of the OPR3‐dependent pathway. Moreover, we identified a negative correlation between dn‐iso‐OPDA and JA‐Ile in land plants, which supports that in bryophytes and lycophytes dn‐iso‐OPDA represents the analogous hormone to JA‐Ile in other vascular plants.
ArabidopsisSRPKII family proteins regulate flowering via phosphorylation of SR proteins and effects on gene expression and alternative splicing
Authors: Wang Tongtong , Wang Xiaofeng , Wang Haiyan , Yu Chao , Xiao Chengyun , Zhao Yiwu , Han Huanan , Zhao Shuangshuang , Shao Qun , Zhu Jianhua , Zhao Yanxiu , Wang Pingping , Ma Changle 
Published: March 2023

Summary
Alternative splicing of pre‐mRNAs is crucial for plant growth and development. Serine/arginine‐rich (SR) proteins are a conserved family of RNA‐binding proteins that are critical for both constitutive and alternative splicing. However, how phosphorylation of SR proteins regulates gene transcription and alternative splicing during plant development is poorly understood.
We found that the Arabidopsis thaliana L. SR protein‐specific kinase II family proteins (SRPKIIs) play an important role in plant development, including flowering. SRPKIIs regulate the phosphorylation status of a subset of specific SR proteins, including SR45 and SC35, which subsequently mediates their subcellular localization.
A phospho‐dead SR45 mutant inhibits the assembly of the apoptosis‐and splicing‐associated protein complex and thereby upregulates the expression of FLOWERING LOCUS C (FLC) via epigenetic modification. The splicing efficiency of FLC introns was significantly increased in the shoot apex of the srpkii mutant. Transcriptomic analysis revealed that SRPKIIs regulate the alternative splicing of c. 400 genes, which largely overlap with those regulated by SR45 and SC35‐SCL family proteins.
In summary, we found that Arabidopsis SRPKIIs specifically affect the phosphorylation status of a subset SR proteins and regulate the expression and alternative splicing of FLC to control flowering time.
Cloning and functional analysis of the root‐knot nematode resistance gene NtRk1 in tobacco
Authors: Zhang Luyang , Xu Zhiqiang , Jiang Zhimin , Chen Xiaoxiang , Li Bo , Xu Liping , Zhang Zhiqiang 
Published: March 2023

AbstractTobacco (Nicotiana tabacum L.) is an economically important crop worldwide. Root‐knot nematodes (RKNs) are responsible for yield losses in tobacco and other crops, such as tomato, potato, peanut, and soybean. Therefore, screening for resistance genes that can prevent RKN infestation and the associated damage is crucial. However, there is no report of cloning tobacco RKN resistance genes to date. Here, we cloned the tobacco RKN resistance gene NtRk1 from the resistant variety TI706, using rapid amplification of cDNA ends. NtRk1 has high homology with other RKN resistance genes (CaMi in pepper, Mi‐1.1 and Mi‐1.2 in tomato). Under normal conditions, NtRk1 was barely expressed in the roots; however, following RKN infection, its expression level rapidly increased. Overexpression of NtRk1 in the susceptible cultivar “Changbohuang” enhanced its resistance to Meloidogyne incognita, while RNA interference of NtRk1 in the resistant cultivar K326 resulted in its susceptibility to M. incognita. Moreover, compared with resistant variety K326, we found the salicylic acid and jasmonic acid contents of RNAi plants decreased after inoculation with M. incognita, and confirmed that the function of NtRk1 is related to these phytohormones. These findings indicate that NtRk1 is an RKN resistance gene, which is abundantly expressed in response to RKN infection and may enhance host defense responses by elevating salicylic acid and jasmonic acid levels.
Greening energy governance through agencification in the Global South: Drivers and implications
Authors: Prontera Andrea , Rubino Alessandro 
Published: March 2023

AbstractThis article offers the first comprehensive analysis of the emergent modes for greening electricity governance through agencification in the Global South by examining the drivers and role of renewable energy agencies (REAs) in various countries in the Middle East and North Africa region. Furthermore, the article illustrates the impact of this form of agencification on the deployment of renewables and the “democratization” of energy governance. We found that the diffusion of REAs is facilitated by the intermediation of international and transnational actors, whereas their role in national energy governance is constrained by the fossil fuels rentier political economy. As an institutional strategy for greening energy governance, agencification has the potential to foster the deployment of renewables: agencies can catalyze external funding overcoming regulatory and policy barriers. However, this strategy can reproduce top‐down approaches to policymaking, hindering the “democratic” potentials of the energy transition.
Hereditary thrombocytopenia with platelet sialic acid deficiency and mutations in the GNE genes
Authors: Montcrieff Caitlin , Ferreira Karen A. , Cheves Tracey A. , Massingham Lauren J. , Sweeney Joseph D. 
Published: March 2023

AbstractBackgroundThe inherited macrothrombocytopenias are rare disorders and the underlying cause can be identified in many cases but in some, this can remain enigmatic. Platelet transfusions are often administered during hemorrhagic events.MethodsA patient with previously unexplained inherited macrothrombocytopenia with a platelet count between 3–20 × 109/L is described in which studies were performed using exome sequencing (ES) and platelet flow cytometry.ResultsBoth the hemoglobin and white cell counts were normal. ES revealed two suspicious variants, one likely pathogenic and one a variant of uncertain significance, in the UDP‐N‐acetylglucosamine 2‐epimerase/N‐acetylmannosamine kinase (GNE) gene, and flow cytometry showed diminished expression of surface platelet sialic acid (about 5%) but normal red cell sialic acid. The Thrombopoietin (TPO) level was low, and the patient responded to TPO‐mimetic treatment with an increase in the platelet count.ConclusionTwo variants in the GNE gene were able to be upgraded to pathogenic with apparently restricted expression to the megakaryocyte lineage. Platelet transfusion may be avoided in these patients with TPO‐mimetic treatment.
Ballistic trauma to the axial skeleton in 13 animals
Authors: Linder Jessica , Daniel Jonathon , Vadurro Francesca , Londono Leonel , Beasley Michaela , Garcia Gabriel A. , Carrera‐Justiz Sheila 
Published: March 2023

AbstractObjectiveTo describe the clinical presentation and outcome of small animals that sustained ballistic trauma to the axial skeleton.DesignRetrospective, multicenter observational study.SettingTwo university teaching hospitals.AnimalsTwelve client‐owned dogs and 1 client‐owned cat sustaining ballistic trauma to the axial skeleton.InterventionsNone.Measurements and Main ResultsThirteen animals sustaining ballistic trauma to the axial skeleton with complete medical records were included in this study. Twelve of 13 animals survived to discharge; 1 animal was euthanized shortly after presentation. Two animals had ophthalmic abnormalities, 9 animals had neurologic lesions, and 2 animals had no significant ophthalmic or neurologic deficits. Neurolocalization of injury included peripheral vestibular (n = 1), multifocal brain (n = 1), brainstem (n = 1), C1–C5 (n = 1), C6–T2 (n = 1), T3–L3 (n = 3), and L4–S3 (n = 1). Seven dogs underwent surgical intervention: 5 neurosurgical, 1 enucleation, and 1 laparotomy. Median hospitalization time was 6 days with a range from 1 to 31 days.ConclusionsOverall prognosis and outcome are variable and dependent on specific injury location and degree of injury.
Successful management of pelvic urethral strictures with balloon dilation in 3 cats
Authors: Hammond Tara N. , King Ryan G. , Danielson Kelson C. 
Published: March 2023

AbstractObjectiveTo describe the successful management of pelvic urethral strictures in 3 young cats (1 after perineal urethrostomy, 1 after a third‐time urethral obstruction, and 1 with prolonged lower urinary tract signs post‐urethral obstruction) using balloon dilation and a short‐term, indwelling urethral catheter.Case SummariesA 9‐month‐old neutered male domestic longhair cat with a urethral obstruction and a suspected congenitally narrowed urethra was treated via perineal urethrostomy. The cat later developed acute kidney injury, multidrug‐resistant urinary tract infections, and a pelvic urethral stricture. A second case, a 2.3‐year‐old neutered male domestic shorthair cat, developed a stricture of the pelvic urethra after multiple obstructions and catheterizations. A third case, a 1.2‐year‐old neutered male domestic shorthair cat, had persistent and prolonged lower urinary tract signs after treatment for a urinary obstruction. The cat also had an abnormally small urethral opening and was ultimately found to have a proximal urethral stricture. The strictures in all 3 cases were successfully treated with a combination of fluoroscopic‐guided balloon dilation and short‐term indwelling urethral catheterization while managing any present infection.New or Unique Information ProvidedTo the authors’ knowledge, this is the first published case series of successful balloon dilations paired with the use of a short‐term indwelling urethral catheter in cats that developed urethral strictures after catheterization due to urinary obstructions. This procedure appears safe and well tolerated and appears to offer a long‐term, cost‐effective solution to urethral strictures at the level of the pelvis.
Organizational factors and safe food handling by food service workers: a scoping review protocol
Authors: Nascimento Letícia Guimarães Perdomo , Ferreira Silvana Margarida Benevides , Palacio Fabiana Gulin Longhi , Takahashi Juliana , Puschel Vilanice Alves de Araújo , da Silva Ageo Mario Candido , da Cunha Diogo Thimoteo 
Published: March 2023

Objective:
The objective of this scoping review is to explore and map the scientific evidence on organizational factors related to safe food handling by food service workers to prevent foodborne disease.

Introduction:
According to the World Health Organization, more than 600 million people become ill each year due to foodborne diseases. Improper food handling is one of the main causes of such diseases. Despite the growing literature on safe food handling, many studies focus on assessing and discussing cognitive variables, such as food safety knowledge. This indicates a need to study this topic from a new perspective, for example, through its organizational factors.

Inclusion criteria:
The review will include quantitative and qualitative studies on food handlers working in food services. Food handlers involved in industrial processing, planting, harvesting, and working on the street (street food) or in the home will be excluded.

Methods:
This review will use the JBI methodology for scoping reviews to identify published and unpublished studies in all languages, with no time limit. The following databases will be searched: Scopus, CINAHL, Embase, Web of Science, MEDLINE, PsycINFO, and Food Science and Technology Abstracts. A structured search for gray literature will also be conducted. Titles, abstracts, and full texts of articles will be screened by 2 reviewers for inclusion, with discrepancies resolved by a third reviewer. Data will be extracted using a standardized form. The results will be summarized in tabular or graphical format, accompanied by a narrative summary.

Key details of this review project are available in Open Science Framework:

https://osf.io/hxfe4
Hybrid Kinematic-Dynamic Sideslip and Friction Estimation
Authors: Carnier Stefano , Corno Matteo , Savaresi Sergio M. 
Published: March 2023

AbstractVehicle sideslip and tyre/road friction are crucial variables for advanced vehicle stability control systems. Estimation is required since direct measurement through sensors is costly and unreliable. In this paper, we develop and validate a sideslip estimator robust to unknown road grip conditions. Particularly, the paper addresses the problem of rapid tyre/road friction adaptation when sudden road condition variations happen. The algorithm is based on a hybrid kinematic-dynamic closed-loop observer augmented with a tyre/road friction classifier that reinitializes the states of the estimator when a change of friction is detected. Extensive experiments on a four wheel drive electric vehicle carried out on different roads quantitatively validate the approach. The architecture guarantees accurate estimation on dry and wet asphalt and snow terrain with a maximum sideslip estimation error lower than 1.5 deg. The classifier correctly recognizes 87% of the friction changes; wrongly classifies 2% of the friction changes while it is unable to detect the change in 11% of the cases. The missed detections are due to the fact that the algorithm requires a certain level of vehicle excitation to detect a change of friction. The average classification time is 1.6 s. The tests also indicate the advantages of the friction classifiers on the sideslip estimation error.