bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is a giant incisional hernia a contraindication for laparoscopic cholecystectomy?
Authors: Boyer Nicolas , Koliakos Nikolaos , Pau Luca , Poras Mathilde , Maréchal Marie-Therese , Farinella Eleonora 
Published: June 2023

Abstract
Laparoscopic cholecystectomy (LC) is one of the most commonly performed surgical procedures worldwide. A previous abdominal operation is not considered a significant risk factor for conversion to open cholecystectomy. We describe the case of an 80-year-old woman with a surgical history of a giant uncomplicated incisional midline hernia presenting at our department with choledocholithiasis and acute cholangitis. After an ERCP with extraction of common bile duct stones, a LC was planned. The first trocar was inserted in the right midclavicular line, using an open technique and a careful inspection of the abdominal cavity and the hernia sac content. An uncomplicated cholecystectomy was performed and the postoperative course was uneventful.
‘Chest gossypiboma after spinal surgery, not so easy to forget’
Authors: Endara Santiago A , Dávalos Gerardo A , Zamora E Elizabeth , Redrobán Ligia M , Molina Gabriel A 
Published: June 2023

Abstract
During any surgical procedure, complications may arise, some of which are fortuitous, whereas others, unfortunately, occur because of errors of the surgical team. Fortunately, most are minor and do not affect the patient’s recovery, but others can cause severe morbidity and even mortality. A retained cotton or gauze surgical sponge inadvertently left in the body during an operation is known as a gossypiboma. This dreadful oversight is a marked complication that can cause serious postoperative complications, a severe economic burden on the healthcare system, and many medicolegal implications. We report the case of a 30-year-old male, who suffered a spinal fracture which was repaired through an anterior fixation approach 12 years ago in a local state hospital without complications. Suddenly, he presented with chest pain and cough, and sought medical attention. An 8 × 5 × 8 cm low-density heterogeneous mass was discovered on his chest; after successful surgery, a gossypiboma formed by several gauzes without radiopaque markers was discovered.
Idiopathic pneumoperitoneum in a single case: an unusual entity
Authors: Sodade Oluwafolaranmi E , Austin Cindy L , Draper Brian B 
Published: June 2023

Abstract
Idiopathic pneumoperitoneum (IP) cases are rare and presents with varying symptoms, which makes propositions of standard treatments, clinically impracticable. There are limited IP therapies in the literature, necessitating a need, to continually highlight unique cases for the purpose of clinical education and training. This case describes an IP and management of a 34-year-old male who recently underwent a laparoscopic cholecystectomy. Patient presented to the emergency room with recurrent gastrointestinal (GI) symptoms. Despite two negative exploratory laparotomies without confirmatory evidence of GI perforations, the GI symptoms persisted, making it an unusual case. The surgeons elected to a multispecialty approach, detailing patient-specific symptoms, and corresponding treatments of the case. Based on the successful outcome of this patient, detailed knowledge of medical history, repeated physical assessments and patient-specific and comprehensive approach was shown to reduce unnecessary exploratory laparotomy, improved clinical outcomes and decrease in complications.
New diagnosis of conversion disorder following anterior lumbar interbody fusion: a case report
Authors: Mitchell Brendan P , Bianco Jake M , Kim Faith M G , Whitaker M Camden 
Published: June 2023

AbstractNeurologic deficit after lumbar spine surgery is a rare and serious complication that must be promptly diagnosed and treated to avoid long-term neurologic disability. Anterior lumbar interbody fusion (ALIF) is an effective technique for the treatment of recurrent disc herniation and lumbar disc degeneration. This case report describes a 20-year-old female with L5-S1 recurrent disc herniation and lumbar degeneration. She underwent an L5-S1 ALIF complicated by post-operative lower left extremity paralysis. Revision surgery with downsizing of the ALIF cage was performed with normal neuromonitoring throughout the procedure. The patient displayed persistent post-operative neurologic deficits despite no evidence of central or foraminal compression. Patient was later diagnosed with conversion disorder by neurology during her hospitalization. This case report presents the initial diagnosis of conversion disorder after a routine ALIF procedure, which led to surgical re-exploration and prolonged inpatient hospital stay. Psychiatric diagnoses must be considered when neurologic deficits are present with no apparent organic cause.
A rare case of benign pneumatosis intestinalis in Sjogren’s syndrome
Authors: Tsang Chi F , Davis Brandon , Chan Daniel L , Yeo David 
Published: June 2023

Abstract
Pneumatosis intestinalis (PI)—the presence of intramural bowel gas—is an uncommon radiological finding, the severity of which depends on the underlying pathological process, ranging from benign disease to life-threatening ischaemia and intra-abdominal sepsis. PI has been described in systemic sclerosis and mixed connective tissue disease; however, few cases have been reported in Sjogren’s syndrome (SjS). The exact pathogenesis of PI in systemic connective tissue disorders is not fully understood and likely multifactorial. We have described a unique case of PI without evidence of peritonitis in a stable patient with long-standing SjS managed non-operatively. An awareness of such benign PI, particularly amongst patients with systemic connective tissue disease, is crucial for diagnostic accuracy and safe patient care, particularly in preventing unnecessary surgical intervention.
Evaluation of the antifungal and antibiofilm activity of postbiotics derived fromLactobacillus spp. onPenicillium expansoum in vitroand in food model
Authors: Khani Nader , Soleimani Roya Abedi , Milani Payam Gonbari , Rad Aziz Homayouni 
Published: June 2023

AbstractFood degradation made by mycotoxigenic molds represents a significant challenge too food security. Postbiotics are associated with soluble compounds liberated by living bacterial cells or their construction release after lysis, and these metabolites offer the host biological action and specific physiological benefits. In this work, the postbiotics from tree strains of Lactobacillus spp. (Limosilactobacillus reuteri ATCC 367, Lacticaseibacillus casei431and Levilactobacillus brevisATCC) were lyophilized, filtered, and tested to evaluate the antimicrobial and anti-biofilm activity in vitro and milk against P. expansoum. Also, to assess the antioxidant efficacy and the free radical scavenging possibility of the postbiotic, DPPH, and ABTS + methods were used. Antimicrobial activity and biofilm removal activity of postbiotics depended on the Lactobacillus strains used. The minimum inhibitory concentration (MIC) of the prepared postbiotic was determined to be 70ug/ml. The lowest minimum effective concentration (MEC) of postbiotics were significantly differed, in the food matrix, and a low MEC index (100 mg/ml) was detected for postbiotic of L. brevis. Postbiotics derived from L. brevis showed the highest antimicrobial activity compared to L. casei and L. reuteri. The postbiotic extracted from Lactobacillus strain may have functional properties (potential antimicrobial and anti-biofilm) in vitro and food models.
The impact of smartphone app–based interventions on adolescents’ dietary intake: a systematic review and evaluation of equity factor reporting in intervention studies
Authors: Schaafsma Holly N , Jantzi Heather A , Seabrook Jamie A , McEachern Louise W , Burke Shauna M , Irwin Jennifer D , Gilliland Jason A 
Published: June 2023

Abstract

Context
Adolescence is a critical stage for improving nutrition. The popularity of smartphones makes them an ideal platform for administering interventions to adolescents. A systematic review has yet to assess the impact of smartphone app–based interventions exclusively on adolescents’ dietary intake. Furthermore, despite the impact of equity factors on dietary intake and the claim for mobile health of increased accessibility, there is minimal research on the reporting of equity factors in the evaluation of smartphone app–based nutrition-intervention research.

Objectives
This systematic review examines the effectiveness of smartphone app–based interventions on adolescents’ dietary intake and the frequency with which equity factors and statistical analyses specific to equity factors are reported in these intervention studies.

Data Sources
Databases (ie, Scopus, CINAHL, EMBASE, MEDLINE, PsycINFO, ERIC, and Cochrane Central Register for Randomized Control Trials) were searched for studies published from January 2008 to October 2022. Smartphone app–based intervention studies that were nutrition focused, evaluated at least 1 dietary intake variable, and included participants with a mean age between 10 and 19 years were included. All geographic locations were included.

Data Extraction and Analysis
Study characteristics, intervention results, and reported equity factors were extracted. Because of the heterogeneity of dietary outcomes, findings were reported as a narrative synthesis.

Conclusion
In total, 3087 studies were retrieved, 14 of which met the inclusion criteria. Eleven studies reported a statistically significant improvement in at least 1 dietary outcome because of the intervention. Reporting of at least 1 equity factor across articles’ Introduction, Methods, Results, and Discussion sections was minimal (n = 5), and statistical analyses specific to equity factors were rare, occurring in only 4 of the 14 included studies. Future interventions should include a measurement of intervention adherence and report the impact of equity factors on the effectiveness and applicability of interventions for equity-deserving groups.
Does Color Matter: Review Article of Skin Color, Power, and Politics in America
Authors: Masuoka Natalie 
Published: June 2023

Abstract
This is a review article of Skin Color, Power, and Politics in America by Mara Cecilia Ostfeld and Nicole Yadon, published by Russell Sage Foundation in 2022. The core premise of the book argues that skin color is another important dimension of the construction of race in America and that it can also provide insight into how individuals experience the world. This article provides an overview of the book and offers reflections on its interventions into the fields of race, ethnicity and politics, public opinion, and political psychology.
PLAAT1 deficiency alleviates high‐fat diet‐induced hepatic lipid accumulation in mice
Authors: Rahman S. M. Khaledur , Sasaki Sumire , Uyama Toru , Hussain Zahir , Sikder Mohammad Mamun , Saiga Hiroyuki , Ohmura‐Hoshino Mari , Ohta Ken‐ichi , Miki Yoshimi , Hoshino Katsuaki , Ueno Masaki , Murakami Makoto , Ueda Natsuo 
Published: June 2023

AbstractThe phospholipase A and acyltransferase (PLAAT) family is composed of three isoforms in mice (PLAAT1, 3, and 5), all of which function as phospholipid‐metabolizing enzymes exhibiting phospholipase A1/A2 and acyltransferase activities. Plaat3‐deficient (Plaat3−/−) mice were previously reported to show lean phenotype and remarkable hepatic fat accumulation under high‐fat diet (HFD) feeding, while Plaat1−/− mice have not been analyzed. In the present study, we generated Plaat1−/− mice and investigated the effects of PLAAT1 deficiency on HFD‐induced obesity, hepatic lipid accumulation, and insulin resistance. After HFD treatment, PLAAT1 deficiency caused a lower body weight gain compared to wild‐type mice. Plaat1−/− mice also showed reduced liver weight with negligible hepatic lipid accumulation. In accordance with these findings, PLAAT1 deficiency improved HFD‐induced hepatic dysfunction and lipid metabolism disorders. Lipidomics analysis in the liver revealed that in Plaat1−/− mice, the levels of various glycerophospholipids tended to increase, while all classes of lysophospholipids examined tended to decrease, suggesting that PLAAT1 functions as phospholipase A1/A2 in the liver. Interestingly, the HFD treatment of wild‐type mice significantly increased the mRNA level of PLAAT1 in the liver. Furthermore, the deficiency did not appear to elevate the risk of insulin resistance in contrast to PLAAT3 deficiency. These results suggested that the suppression of PLAAT1 improves HFD‐induced overweight and concomitant hepatic lipid accumulation.
Proximity‐dependent biotin identification links cholesterol catabolism with branched‐chain amino acid degradation in Mycobacterium smegmatis
Authors: Veyron‐Churlet Romain , Lecher Sophie , Lacoste Anne‐Sophie , Saliou Jean‐Michel , Locht Camille 
Published: June 2023

AbstractCholesterol is a crucial component in Mycobacterium tuberculosis virulence as it is required for phagocytosis of mycobacteria by macrophages. In addition, the tubercle bacilli can grow using cholesterol as the sole carbon source. Thus, cholesterol catabolism represents a valuable target for the development of new antitubercular drugs. However, the molecular partners of cholesterol catabolism remain elusive in mycobacteria. Here, we focused on HsaC and HsaD, enzymes involved in two consecutive steps of cholesterol ring degradation and identified putative partners, using a BirA‐based proximity‐dependent biotin identification (BioID) approach in Mycobacterium smegmatis. In rich medium, the fusion protein BirA‐HsaD was able to fish the endogenous cognate HsaC, thus validating this approach to study protein–protein interactions and to infer metabolic channeling of cholesterol ring degradation. In chemically defined medium, both HsaC and HsaD interacted with four proteins, BkdA, BkdB, BkdC, and MSMEG_1634. BkdA, BkdB, and BkdC are enzymes that participate in the degradation of branched‐chain amino acids. As cholesterol and branched‐chain amino acid catabolism both generate propionyl‐CoA, which is a toxic metabolite for mycobacteria, this interconnection suggests a compartmentalization to avoid dissemination of propionyl‐CoA into the mycobacterial cytosol. Moreover, the BioID approach allowed us to decipher the interactome of MSMEG_1634 and MSMEG_6518, two proteins of unknown function, which are proximal to the enzymes involved in cholesterol and branched‐chain amino acid catabolism. In conclusion, BioID is a powerful tool to characterize protein–protein interactions and to decipher the interconnections between different metabolic pathways, thereby facilitating the identification of new mycobacterial targets.
Transmorphic phage‐guided systemic delivery of TNFα gene for the treatment of human pediatric medulloblastoma
Authors: Al‐Bahrani Mariam , Asavarut Paladd , Waramit Sajee , Suwan Keittisak , Hajitou Amin 
Published: June 2023

AbstractMedulloblastoma is the most common childhood brain tumor with an unfavorable prognosis and limited options of harmful treatments that are associated with devastating long‐term side effects. Therefore, the development of safe, noninvasive, and effective therapeutic approaches is required to save the quality of life of young medulloblastoma survivors. We postulated that therapeutic targeting is a solution. Thus, we used a recently designed tumor‐targeted bacteriophage (phage)‐derived particle, named transmorphic phage/AAV, TPA, to deliver a transgene expressing the tumor necrosis factor‐alpha (TNFα) for targeted systemic therapy of medulloblastoma. This vector was engineered to display the double‐cyclic RGD4C ligand to selectively target tumors after intravenous administration. Furthermore, the lack of native phage tropism in mammalian cells warrants safe and selective systemic delivery to the tumor microenvironment. In vitro RGD4C.TPA.TNFα treatment of human medulloblastoma cells generated efficient and selective TNFα expression, subsequently triggering cell death. Combination with the chemotherapeutic drug cisplatin used clinically against medulloblastoma resulted in augmented effect through the enhancement of TNFα gene expression. Systemic administration of RGD4C.TPA.TNFα to mice‐bearing subcutaneous medulloblastoma xenografts resulted in selective tumor homing of these particles and consequently, targeted tumor expression of TNFα, apoptosis, and destruction of the tumor vasculature. Thus, our RGD4C.TPA.TNFα particle provides selective and efficient systemic delivery of TNFα to medulloblastoma, yielding a potential TNFα anti‐medulloblastoma therapy while sparing healthy tissues from the systemic toxicity of this cytokine.
Disruption of maternal ACKR3 has profound effects on embryos and offspring
Authors: Fukuoka Ayumi , Wilson Gillian J. , Pitmon Elise , Foley Lily Koumbas , Johnsson Hanna , Pingen Marieke , Graham Gerard J. 
Published: June 2023

AbstractACKR3, scavenges and degrades the stem cell recruiting chemokine CXCL12 which is essential for proper embryonic, and in particular hematopoietic, development. Here we demonstrate strong expression of ACKR3 on trophoblasts. Using a pharmacological blocker we demonstrate that ACKR3 is essential for preventing movement of CXCL12 from the mother to the embryo with elevated plasma CXCL12 levels being detected in embryos from ACKR3-blocker treated mothers. Mice born to mothers treated with the blocker are lighter and shorter than those born to vehicle-treated mothers and, in addition, display profound anaemia associated with a markedly reduced bone marrow hematopoietic stem cell population. Importantly, whilst the hematopoietic abnormalities are corrected as mice age, our studies reveal a postnatal window during which offspring of ACKR3 blocker treated mice are unable to mount effective inflammatory responses to inflammatory/infectious stimuli. Overall, these data demonstrate that ACKR3 is essential for preventing CXCL12 transfer from mother to embryo and for ensuring properly regulated CXCL12 control over the development of the hematopoietic system.
OverlookedCandida glabratapetites are echinocandin tolerant, induce host inflammatory responses, and display poorin vivofitness
Authors: Arastehfar Amir , Daneshnia Farnaz , Hovhannisyan Hrant , Fuentes Diego , Cabrera Nathaly , Quintin Christopher , Ilkit Macit , Ünal Nevzat , Hilmioğlu-Polat Suleyha , Jabeen Kauser , Zaka Sadaf , Desai Jigar V. , Lass-Flörl Cornelia , Shor Erika , Gabaldon Toni , Perlin David S. 
Published: June 2023

AbstractSmall colony variants (SCVs) are relatively common among some bacterial species and are associated with poor prognosis and recalcitrant infections. Similarly,Candida glabrata– a major intracellular fungal pathogen – produces small and slow-growing respiratory-deficient colonies, termed “petite.” Despite reports of clinical petiteC.glabratastrains, our understanding of petite behavior in the host remains obscure. Moreover, controversies exist regarding in-host petite fitness and its clinical relevance. Herein, we employed whole-genome sequencing (WGS), dual-RNAseq, and extensiveex vivoandin vivostudies to fill this knowledge gap. WGS identified multiple petite-specific mutations in nuclear and mitochondrially-encoded genes. Consistent with dual-RNAseq data, petiteC.glabratacells did not replicate inside host macrophages and were outcompeted by their non-petite parents in macrophages and in gut colonization and systemic infection mouse models. The intracellular petites showed hallmarks of drug tolerance and were relatively insensitive to the fungicidal activity of echinocandin drugs. Petite-infected macrophages exhibited a pro-inflammatory and type I IFN-skewed transcriptional program. Interrogation of internationalC.glabratablood isolates (n=1000) showed that petite prevalence varies by country, albeit at an overall low prevalence (0–3.5%). Collectively, our study sheds new light on the genetic basis, drug susceptibility, clinical prevalence, and host-pathogen responses of a clinically overlooked phenotype in a major fungal pathogen.ImportanceCandida glabratais a major fungal pathogen, which is able to lose mitochondria and form small and slow-growing colonies, called “petite”. This attenuated growth rate has created controversies and questioned the clinical importance of petiteness. Herein, we have employed multiple omicstechnologies and in vivo mouse models to critically assess the clinical importance of petite phenotype. Our WGS identifies multiple genes potentially underpinning petite phenotype. Interestingly, petiteC. glabratacells engulfed by macrophages are dormant and therefore are not killed by the frontline antifungal drugs. Interestingly, macrophages infected with petite cells mount distinct transcriptomic responses. Consistent with our ex-vivo observations, mitochondrial-proficient parental strains outcompete petites during systemic and gut colonization. Retrospective examination ofC. glabrataisolates identified petite prevalence a rare entity, can significantly vary from country to country. Collectively, our study overcomes the existing controversies and provides novel insights regarding the clinical relevance of petiteC. glabrataisolates.
CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing
Authors: Carbonell-Sala Silvia , Lagarde Julien , Nishiyori Hiromi , Palumbo Emilio , Arnan Carme , Takahashi Hazuki , Carninci Piero , Uszczynska-Ratajczak Barbara , Guigó Roderic 
Published: June 2023

ABSTRACTLong-read RNA sequencing is essential to produce accurate and exhaustive annotation of eukaryotic genomes. Despite advancements in throughput and accuracy, achieving reliable end-to-end identification of RNA transcripts remains a challenge for long-read sequencing methods. To address this limitation, we developed CapTrap-seq, a cDNA library preparation method, which combines the Cap-trapping strategy with oligo(dT) priming to detect 5’capped, full-length transcripts, together with the data processing pipeline LyRic. We benchmarked CapTrap-seq and other popular RNA-seq library preparation protocols in a number of human tissues using both ONT and PacBio sequencing. To assess the accuracy of the transcript models produced, we introduced a capping strategy for synthetic RNA spike-in sequences that mimics the natural 5’cap formation in RNA spike-in molecules. We found that the vast majority (up to 90%) of transcript models that LyRic derives from CapTrap-seq reads are full-length. This makes it possible to produce highly accurate annotations with minimal human intervention.