bluuebunny/crossref_metadata_2025_split huggingface dataset:
Duplex RTqPCRs for detection and relative quantification of SARS-CoV-2 variants of concern (VOC)
Authors: Carcereny Albert , Garcia-Pedemonte David , Bosch Albert , Pintó Rosa M , Guix Susana 
Published: February 2023

Abstract

During the evolution of the SARS-CoV-2 pandemic, new variants of the virus have emerged and spread worldwide. The increased transmissibility and proclivity of some of these variants to cause more serious disease threatens public health responses against the virus, and they are classified as variants of concern (Variants of Concern, VOCs). While Next-Generation-Sequencing (NGS) is the gold standard to identify VOC, it cannot always be rapidly implemented in some settings to provide information as an early warning tool. Duplex quantitative real time RTqPCR assays offer a sensitive and easy-to-use tool to detect, discriminate, and estimate relative proportions of SARS-CoV-2 variants containing VOC-specific signature mutations from variants lacking it, using allelic discrimination probes. We developed seven multiplexed RTqPCR assays that can detect Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), Omicron (B.1.1.529), Omicron BA.1 (B.1.1.529.1) and Omicron BA.2 (B.1.1.529.2) VOCs by targeting 21765_21770DelTACATG, 22281_22289DelCTTTACTTG, 28262_28263InsAACA, 22029_22034DelAGTTCA, 28362_28370DelGAGAACGCA, 22121InsGAGCCAGAA, and 21633_21641DelTACCCCCTG deletions/insertions in their genomes, respectively. Alpha, Beta, Delta, Omicron BA.1 and Omicron BA.2 markers are mapped to the S gene (residues 69/70, 241/243, 157/158, 214, and 25/27 respectively), Gamma insertion is located between the end of ORF8 and the beginning of N gene, and Omicron (B.1.1.529) deletion is mapped to the N gene (residues 31/33). The duplex RTqPCR assay targeting 21765-21770DelTACATG mutation affecting residues HV69/70 has been previously used to estimate the relative proportion of Alpha VOC (Carcereny et al., 2021), and is also suitable to estimate the proportions of Omicron BA.4 and BA.5. All duplex RTqPCR assays targeting signature mutations of VOCs may be used as a complementary tool to NGS for rapid variant tracking and surveillance in wastewater-based epidemiology.
A Novel Multilayer Framework for Fake News Detection Based on Multivariate Data Features Mining
Authors: Zhi Xiaofan , Xue Li , Zhao Bo 
Published: February 2023

Abstract
The spread of fake news may disturb the financial markets in the background of program trading. Nowadays, news often contains different types of data and circulate on various new media platforms, which challenges to existing fake news detection models. It is not just because the features of fake news are getting more complicated, but also traditional methods often ignore that the user comments may also provide valuable information for this task. To address these gaps, we present a multivariate data feature mining based multilayer framework (MDFM-MF). In the bottom layer, different news carriers are uniformly converted into text data. The middle layer then extracts the opinion, sentiment, user and propagation features from the text data. Eventually, fake news detection is realized based on the BERT model in the top layer. The experiment results show that our proposed model has good performance on detection task.
Insulin-like Growth Factor-1 Gene Polymorphism and Its Effect on Egg Quality Traits of Funaab Alpha, Kuroiler and Sasso Chicken Reared in Hot Humid Tropics
Authors: Wheto Mathew , Chima Nkiruka Goodness , Sanda Adeyinka Julius , Ilori Babatunde Moses , Ayo-Ajasa Olapeju Yemsis , Oke Emmanuel Oyegunle , Iyasere Oluwaseun S. , Adebambo Ayotunde Olutumininu , Adebambo Olufunmilayo Ayoka 
Published: February 2023

AbstractA study was conducted on the effect of gene polymorphism ofInsulin like Growth Factor-1 (IGFI)on egg quality traits in five chicken genotype. A total of 250 chicken comprising 150 FUNAAB-Alpha (50 Normal feather, 50 Naked neck and 50 Frizzle feather), 50 Kuroiler and 50 Sasso were used for this experiment. The chicks were generated through artificial insemination and were raised to maturity on deep litter system. At point of lay, 30 hens per genotype were selected and transferred into a battery cage of one unit per bird. Data was collected on the egg quality traits, 30 eggs for each genotype was collected, the following parameters were determined: egg (weight, length, width), shape index, shell thickness, albumen (height, weight), yolk weight and color, ratio of (shell, yolk, albumen) and Haugh unit. All collected data was subject to analysis of variance using a completely randomized design, of which genotype was the interest factor. At 16 weeks, 1ml of blood was collected from each hen and extraction of genomic DNA from the blood was done. PCR was conducted using the pair of primer and condition as described by Nagaraga,et al. (2000). The PCR amplicons were digested usingPstIrestriction enzymes following the manufacturer’s procedure. The resulting fragments were analyzed using GenAnalyzer (GenAlEx 6.502) was used for the genetic diversity of theIGFIlocus. This data was subject to the PROC GLM of SAS 9.2. Results showed the chicken genotypes significantly (P<0.05) affect all the egg-quality traits except the shell weight, yolk ratio and albumen ratio. TheIGFIgene polymorphism had no significant effect (P>0.05) on egg quality traits for except the egg length and egg width.
Combined exposure of polystyrene microplastics and carbamazepine induced transgenerational effects on the reproduction of Daphnia magna
Authors: He Yide , Zhang Yiqun , Zhou Wei , Freitas Rosa , Zhang Yunhai , Zhang Yongjun 
Published: February 2023

Abstract
Polystyrene microplastics (PS MPs) and carbamazepine (CBZ) are frequently detected in freshwater ecosystems. However, the transgenerational effects of PS MPs and CBZ on the reproduction of aquatic organisms and the corresponding mechanisms are still unclear. In the present study, D. magna was used to evaluate the reproductive toxicity in two consecutive generations (F0, F1). Molting and reproduction parameters, the expression of reproduction, and toxic metabolism genes were examined after 21 d exposure. A significantly enhanced toxicity was observed in the presence of 5 μm PS MPs and CBZ. Chronic exposure results showed that the 5 μm PS MPs alone, CBZ alone, and their mixtures exerted significant reproductive toxicity of D. magna. The results of RT-qPCR showed transcripts of genes related to reproduction (cyp314, ecr-b, cut, vtg1, vtg2, dmrt93b) and toxic metabolism (cyp4, gst) were altered in both the F0 and F1. In addition, for the F0, gene transcriptional changes of reproduction were not fully translated into physiological performance, probably due to the compensatory responses caused by the low dose of PS MPs alone, CBZ alone, and their mixtures. Whereas for the F1, the trade-off between reproduction and toxic metabolism at gene levels was observed, which translated into a significant reduction in the total neonate number of F1. These findings suggest that long-term exposure to MPs and CBZ can cause serious reproduction damage to aquatic animals, which needs to be given sufficient attention.
Two-Port Dry Vitrectomy for Rhegmatogenous Retinal Detachment: a pilot study
Authors: Peiretti Enrico , Caporossi Tomaso , Tatti Filippo , Scampoli Alessandra , Mangoni Lorenzo , Carlà Matteo Mario , Pintor Emanuele Siotto , Carta Valentina , Iovino Claudio , Rizzo Stanislao 
Published: February 2023

Abstract
Objective
To evaluate the safety and efficacy of a new surgical technique for the management of primary rhegmatogenous retinal detachment (RRD), consisting of localized PPV near to the retinal break(s), without infusion line, associated with a drainage of subretinal fluid and cryoretinopexy.
Methods
Multicentric prospective study conducted at the University Hospital of Cagliari and IRCCS Fondazione Policlinico Universitario A. Gemelli, Roma. 20 eyes affected by RRD with the causative retinal break(s) in the superior meridians were enrolled between February 2022 and June 2022. Patients with cataract ≥ 3, aphakia, significant posterior capsule opacification, giant retinal tears, retinal dialysis, history of trauma and PVR ≥ C2 were excluded. All eyes underwent a two-port 25-gauge PPV with localized removal of the vitreous surrounding retinal break(s), followed by 20% SF6 injection and cryopexy. The surgical time was recorded for each procedure. Best corrected visual acuity (BCVA) was measured at baseline and postoperative 6-month.
Results
Primary anatomic success at 6 months was achieved by 85% of patients. No complications occurred, excepted for three (15%) retinal re-detachments. The average surgical time was 8.61 ± 2.16 minutes. Overall, the difference between pre- and last post-operative mean BCVA was statistically significant. (p 0.02)
Conclusions
Two-port dry PPV demonstrated safety and efficacy for the treatment of RRD, reaching 85% of anatomical success rate. Although further studies are necessary to confirm the efficacy and long-term benefit of this treatment, we believe that this surgical technique could be considered a valid and safe alternative for the management of primary RRD.
Sex-associated differences in incentive salience and drinking behavior in a rodent model of alcohol relapse
Authors: Winter Christine , Hakus Aileen , Foo Jerome , Casquero-Veiga Marta , Gül Asude , Hintz Franziska , Rivalan Marion , Winter York , Priller Josef , Hadar Ravit 
Published: February 2023

Abstract
The ability of environmental cues to trigger alcohol-seeking behaviors is thought to facilitate problematic alcohol use. The tendency to attribute incentive salience to cues varies between individuals and may constitute a risk factor for the development of addiction. Understanding the relationship between incentive salience and alcohol addiction may help inform prevention and treatment strategies for addiction. Animal studies have focused predominantly on males of alcohol-preferring strains of rodents. We sought to study the relationship between incentive salience and alcohol addiction using non-preferring rats to model the heterogeneity of human alcohol consumption, investigating both males and females. Here, adult RccHan Wistar rats were subjected to the alcohol deprivation effect (ADE) paradigm, mimicking alcohol relapse in humans. They were given voluntary access to different alcohol solutions in a four-bottle paradigm with repeated interruptions by deprivation and reintroduction phases over a protracted period (5 ADE cycles). Before each ADE cycle, rats were tested in the Pavlovian Conditioned Approach (PCA) paradigm, which quantifies the individual tendency toward a conditional cue and the reward, thus allowing us to trace the process of attributing incentive salience to reward cues. During the final ADE cycle (ADE5), animals were tested for compulsive-like behavior using quinine taste adulteration. Associations between longitudinal drinking patterns, PCA performance, and eventual compulsive-like behavior were analyzed. We investigated sex differences in drinking behavior and PCA performance; females drank significantly more alcohol than males throughout all ADE phases and displayed more sign-tracking (ST) behavior in the PCA, whereas males showed goal-tracking (GT) behavior. PCA phenotypes emerged at the first ADE cycle and remained stable over subsequent cycles. When looking at the correlation between alcohol drinking and PCA performance, high drinkers exhibited more ST behavior, and low drinkers exhibited more GT behavior; initial PCA phenotype was correlated with later alcohol consumption. Our findings indicate a more complex relationship between incentive salience and alcohol addiction than previously suggested and emphasize the importance of considering individual differences and both sexes in preclinical research.
WITHDRAWN: Design of 5G MIMO Aerial With Non-Ionizing Radiation Protection.
Authors: S AJITH A 
Published: February 2023

Abstract
Novel antenna designs that utilise antenna arrays to provide improved coverage, minimise interference,
and boost data-carrying capacity are needed to meet 5G targets. This paper provides an approach for
designing an inverted-L 5G MIMO antenna with SAR (Specific Absorption Rate) calculation. The designed
antenna will be resonating at 5.84GHz, with optimal parameters like reflection coefficient, gain and
voltage standing wave ratio (VSWR). The measured bandwidth is in the range of 3.07GHz to 6.6GHz. The
simulated human body SAR values are 0.925 W/kg, which is less than the ICNIRP-recommended SAR
safety limit of 2 W/kg. The user-friendly CST Microwave Studio Software is used for all simulations and
design.
Effect of the vertical facial pattern on the developmental relationship between the nasal bone and maxillary central incisors
Authors: Shi Jianwei , Alak'hali Mohammed , Cai Dingjun , Guo Qiutong , Cao Yuming , Alhammadi Maged , Mashrah Mubarak Ahmed , Yang Yang 
Published: February 2023

Abstract
Background: This study aimed to investigate the effect of the vertical facial pattern on the developmental relationship between the nasal bone and maxillary central incisors.
Methods: In this retrospective comparative study, the lateral cephalograms of 213 subjects (51 Male; 162 Female) with skeletal Class I malocclusion (aged 18 to 32 years) were classified into three equal groups; hyperdivergent, normodivergent, and hypodivergent facial patterns based on the mandibular plane inclination (S-N/Go-Me). The following measurements were extracted; gradient and length of the nasal bone and maxillary central incisor, the distance from apex and root of the nasal bone, and maxillary central incisor to the true perpendicular from the digitized lateral cephalograms. The significance level was considered at P< 0.05.
Results: The inclination angle and length between nasal bone and maxillary central incisor were positively correlated independent of vertical facial type. The inclination angle of the nasal bone in the hypodivergent group was significantly larger than the other two vertical facial types. The inclination angle of the maxillary central incisor increased successively in the hyperdivergent, normodivergent, and hypodivergent groups. The length of the nasal bone in the hyperdivergent group was significantly longer than that of the hypodivergent and normodivergent groups. The maxillary central incisor length in the hyperdivergent group is significantly longer than in the hypodivergent group.
Conclusion: There was a correlation between nasal bone and maxillary central incisor during the growth and development of the maxillofacial region. In Class I malocclusion subjects, hypodivergent patients were more likely to have a prominent and relatively short nasal bone and maxillary central incisor and vice versa.
Cytokine and chemokine map of peripheral specific immune cell subsets in Parkinson's disease
Authors: Pu Jiali , Jiang SiSi , wang yiling , xu qiuhan , gu luyan , kang ruiqing , yang wenyi , Zhang Baorong , tian jun 
Published: February 2023

Abstract
Background: Peripheral immune cells play a vital role in the development of Parkinson's disease (PD). However, their cytokine and chemokine secretion functions remain unclear.
Objective: To explore the cytokine and chemokine secretion functions of specific immune cell subtypes in drug-naïve patients with PD at different ages of onset. Methods: We included ten early-onset and ten late-onset patients with PD and age-matched healthy controls (HCs). We used mass cytometry to select specific immune cell subsets and evaluate intracellular cytokine and chemokine expression. Statistical tests included t-tests, analysis of variance, bivariate correlation analysis, and linear regression analysis.
Results: Compared with HCs, patients with PD exhibited significantly decreased intracellular pro-inflammatory cytokines and chemokines in selected clusters (e.g., tumor necrosis factor [TNF]-α, interleukin [IL]-8, IL-1β, and CC-chemokine ligand17). Specific cytokines and cell clusters were associated with clinical symptoms. TNF-α played an important role in cognitive impairment. Intracellular TNF-α levels in the naïve CD8+ T-cell cluster C16 (CD57-naïve CD8+T) and natural killer (NK) cell cluster C32 (CD57-CD28-NK) were negatively correlated with Montreal Cognitive Assessment scores. The C16 cluster affected cognitive function and motor symptoms. Increased TNF-α and decreased interferon-γ expression in C16 correlated with increased Unified Parkinson’s Disease Rating Scale III scores in patients with PD.
Conclusions: We developed a more detailed cytokine and chemokine map of peripheral specific CD8+ T-cell and NK cell subsets, which revealed disrupted secretory function in patients with PD and provided unique clues for further mechanistic exploration.
Classification of wearables use cases in the mirror of JCAHO patient safety goals for Hospitals
Authors: Askari Mehdi , Kalankesh Leila R. , Asadzadeh Afsoon , Yousefi-Rad Khatereh 
Published: February 2023

Abstract
Background
Patient safety can be improved by using wearable devices in hospitals. Wearable technology has the potential to provide enormous advantages to healthcare providers and patients. This study aims to explore the use cases of wearable technology in patient safety in hospitals and classify them based on the healthcare wearable taxonomy, focusing on the JCAHO patient safety goals for hospitals.
Materials and Methods
The search strategy was performed in Medline (PubMed), Scopus, and Google Scholar using keywords related to wearable technology and patient safety on September 4, 2021. English papers that presented the applications of wearable technology on patient safety of inpatients were included. A qualitative synthesis was performed by applying the taxonomy of wearables in healthcare and the JCAHO ( Joint Commission on Accreditation of Healthcare Organizations ) goals for hospital patient safety.
Results
Of the 345 papers retrieved, ten were included in this study. The results demonstrated the usefulness of wearable devices in patient safety in hospitals. Monitoring, prevention, and assistive technology were three applications of wearables that had been more commonly reported in the studies. This research showed that the two goals of the JCAHO are the most frequently addressed through the application of wearables in hospitals. These goals include 1) identifying patient safety risks in the hospital by measuring various parameters such as vital signs and 2) reducing patient harm associated with the clinical alarm.
Conclusion
This study provides evidence for the potential applications of wearable technology for improving patient safety in hospitals. Considering the multiple functionalities of the wearables and their potential applications, hospitals could put wearables on their agenda for improving patient safety and quality of care.
Influence of the filter grain morphology on separation efficiency in dielectrophoretic filtration
Authors: Kepper Mariia , Karim Md Nurul , Baune Michael , Thöming Jorg , Pesch Georg R. 
Published: February 2023

Abstract
Recovery of noble materials from waste is essential for industries around the globe. Dielectrophoretic (DEP) filtration, an electrically switchable particle separation technique, can be applied to tackle this challenge. It is highly selective regarding particle size, material, or shape. Expanding the scope of DEP towards high throughput and improving the trapping efficiency are vital to make DEP a viable robust alternative to conventional separation methods. DEP filtration works by selective immobilisation of particles in a porous medium by the action of an inhomogeneous electric field. The field inhomogeneity comes from scattering an electric field at the phase boundary between the particle suspension and the filer surface. In this article, we show how the filter structure affects the DEP separation. We study fixed bed filters of three different grain types and find that the morphology of the grains highly influences the DEP filter efficiency. Specifically, grains with irregular surface structure and high perceived angularity show high separation efficiency. We believe insights into the design of DEP filtration will pave the way towards its application in, e.g., the recovery of valuable materials from electronic waste dust.
Drug-Drug Interaction Potential of SH-1028, a Third-generation EGFR-TKI: In Vitro and Clinical Trials
Authors: Li Xiaoli , Liu Yuyan , Xu Yuanyuan , Ding Jiaxiang , Wang Ying , Shan Rongfang , Liu Yuanyuan , Liu Bingyan , Ding Yuzhou , Xie Jing , Zhou Huan , Wang Zhiqiang , Li Hongtao 
Published: February 2023

Abstract
SH-1028 is an irreversible third-generation EGFR tyrosine kinase inhibitor (EGFR-TKI) for the treatment of locally advanced or metastatic non-small cell lung cancer (NSCLC). Considering the possibility of combination therapy in patients with NSCLC, we investigated the drug-drug interaction (DDI) potential of SH-1028 both in vitro and in clinical trials. The in vitro studies were conducted to determine the potential of SH-1028 as a substrate, inducer, or inhibitor of cytochrome P450 (CYP) subtypes. A phase I drug-drug interaction study in healthy volunteers was performed to evaluate the impact of co-administering rifampicin (a strong CYP3A4 inducer) and itraconazole (a strong CYP3A4 inhibitor) on the pharmacokinetics of SH-1028. The in vitro experiments showed that SH-1028 was mainly metabolized by CYP3A4. The activities of CYP1A2, 2B6, 2C19, 2D6 and 3A4 enzymes were slightly inhibited in vitro with SH-1028. SH-1028 has no obvious induction effect on CYP1A2 and CYP2B6 activities, but has potential induction effect on CYP3A4 mRNA expression. However, SH-1028 may not induce or inhibit human CYPs significantly at the clinically expected dose (200 mg). The geometric mean ratios of pharmacokinetic parameters and their corresponding 90% confidence intervals for SH-1028 in combination and alone did not fall within the range of 80–125%. It is speculated that itraconazole and rifampicin affect the metabolism of SH-1028. In the clinical application of SH-1028, special attention should be paid to the interaction between SH-1028 and drugs or foods that affect the activity of CYP3A4. (Clinical trial registration number: CTR20210558)