bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the Match Patterns between Gag Protein and Primer Binding Site based on Bioinformatics Analysis
Authors: Chen Shaoming 
Published: April 2023

Abstract
Previous studies have suggested that the genomic Gag/Gag-Pol complex is involved in recruiting the LysRS/tRNA complex, and that the selective packaging of the tRNA primer requires HIV-1 Gag and Gag-Pol. Furthermore, in vitro experiments have demonstrated an interaction between LysRS and Gag, and HIV-1 initiates the process of reverse transcription by using LysRS to bind to the genomic RNA at the primer binding site. These findings strongly suggest that the gag protein plays a critical role in matching the primer binding site. In this study, the author investigated the amino acid sequences of Gag proteins of several retroviruses and their matching primer binding sites. The analysis showed that the amino acid sequence ARG of Gag proteins of HTLV-1, HTLV-2, STLV-1, and STLV-2 match their primer binding site GGGGGCTCG in the 3'-to-5' direction, while the amino acid sequence SPR of Gag proteins of HIV-1, HIV-2, SIV, and FIV match their primer binding site GGCGCCCGA in the 3'-to-5' direction. These findings suggest a potential mechanism for the specificity of reverse transcription in retroviruses. However, it is important to note that the primer binding sites of different viruses are extremely stable, and other amino acid sequences of Gag proteins may also match these sites. Further research is needed to confirm this hypothesis. Overall, the study provides valuable insights into the mechanisms of retroviral reverse transcription, and provides an advance for further research in this area.
The need to stabilize the bacterial community present in the cultures of the marine diatom Haslea ostrearia
Authors: Peticca Aurelie , Fodil Mostefa , Chenais Benoit , Sabot Francois , Casse Nathalie 
Published: April 2023

Abstract
Objectives: Haslea ostrearia is a microalgae associated with the marrenine, a blue pigment known for its economic interest when it attaches to oyster gills. Complex to grow under axenic conditions, H. ostrearia probably needs its own bacterial procession to survive. Consequently, this important bacterial presence greatly complicates any study of its ecological, physiological and genomic content.
Results: We therefore tested a first approach of a protocol to control the bacterial concentration without killing the microalgae, by growing four H. ostrearia NCC 532 cultures under two culture conditions: two with and two without antibiotic treatment. We also performed a first bacterial identification method by 16S sequencing adapted to the bacterial community of H. ostrearia. The V3V4 16S region allowed precise identification of bacteria present in H. ostrearia culture. An antibiotic treatment was found as necessary to stabilize the bacterial population of the cultures before the DNA extraction.
DC targeted therapy breaks immune tolerance against neoantigens in refractory lung adenocarcinoma
Authors: Lopez-Rodriguez Lucia , Bourdely Pierre , Terza Federica La , Rospo Giuseppe , Morosi Luciano , Amadio Roberto , Piperno Giulia Maria , Volponi Camilla , Vodret Simone , Joshi Sonal , Giannese Francesca , Lazarevic Dejan , Germano Giovanni , Stoitzner Patrizia , Bardelli Alberto , Dalod Marc , Caronni Nicoletta , Guermonprez Pierre , Benvenuti Federica 
Published: April 2023

Abstract
The efficacy of immune checkpoint blockade (ICB) in NSCLC depends on the tumor mutational burden (TMB). However, a fraction of patients with high TMB and predicted immunogenic neoantigens (neoAgs) do not respond. Here we show that in a model of highly mutated NSCLC, cross-presenting cDC1s are required to induce broad effector CD8+ T cell responses to both strong and weak endogenous neoAgs. Importantly, cDC1 amplification by Flt3L increases immunogenicity of MHC class-I neoepitopes and promotes tumor regression, whilst PD-L1 blockade is ineffective. cDC1 density correlates to CD8+ T cell scores and prognosis, particularly in hypermutated human NSCLC. Single-cell RNA sequencing reveals the molecular determinants of Flt3L-therapy including expansion of immunogenic lung cDC1 and proliferation of cytotoxic CD8+ T cells with reduced exhaustion. We conclude that boosting cDC1 activity is critical to leverage neoAgs content for therapeutic advantage in hypermutated lung tumors that do not respond to ICB.
Ascophyllum nodosum Le Jolis Harvesting Impacts and Management Options Using GPS Tracking of Mechanical Harvesters in Nova Scotia, Canada
Authors: Sharp Glyn J. , Sharp Joshua T 
Published: April 2023

Abstract
Area based management of Ascophyllum nodosum Le Joli in the Canadian Maritimes has advanced over the past 60 years from open buying stations in Bays to quota-based area management under coastal leases. In 1989 the resource was divided into geographical sectors containing .9 to 17.6 ha containing 92 t to 2105 t of harvestable biomass. Sectors are the units of management plans under guidelines of the provincial government. GPS tracking mechanical harvesting to Nova Scotia began in 2017 using a new mechanical harvester in a pilot harvest. GPS tracking allowed the calculation of yield with the time, distance, and cutting swath. Harvests were conducted within target bed polygons of 200 m− 2 to 1280 m− 2. Production per hour averaged 1135 ± 169 kg h− 1 yielding 5.96 ± 1.01 kg m− 2 of swept track. The average exploitation rate within targeted polygons was 33.1 ± 14.5%. This level of geographic resolution of the harvest permits significant improvements in management practices, control of management plans, pre and post assessments of the resource. It is a method of addressing landscape scale questions relating to harvesting impacts.
Jump connection generative adversarial network for fabric defect detection
Authors: Cheng Haoming , Liang Jiuzhen , Liu Hao 
Published: April 2023

Abstract
Among the fabric defect detection methods, unsupervised methods are based on the principle of training a network to restore a fabric image with defects to a flawless image with a consistent background and no visible defects, and to obtain specific information about the defects by comparing the two images for defect detection. However, most of the restored images cannot remove the defective part completely, and the more obvious defects are still visible. To solve the above problem, this paper proposes the jump connection generative adversarial network for fabric defect detection (JCGAN). JCGAN uses a jump connection structure to improve the ability of the network to extract detail information by introducing the detail information from downsampling into the upsampling process. It introduces a low-dimensional loss function to control the training of the network to improve the quality of the generated images. It uses two detection algorithms (SSIM computational detection algorithm, multi-channel defect detection algorithm) to compute the disparity grayscale information of the defected and recovered images separately, and finally fuses the information from both sides to obtain more detailed detection results. Compared with the six commonly used methods, the f-value of JCGAN is improved by 13.40\% on average compared with other methods.
Tau-PET abnormality change along with Alzheimer’s disease: a topological perspective
Authors: Ding Jie , Shen Chushu , Wang Zhenguo , Yang Yongfeng , Fakhri Georges El , Lu Jie , Liang Dong , Zheng Hairong , Zhou Yun , Sun Tao 
Published: April 2023

Abstract
Background: Tau PET imaging is an important biomarker for studying the progression of Alzheimer’s disease. The most commonly utilized quantification index in tau-PET is the standardized uptake value ratio (SUVR). Topological information derived from different brain regions has also been linked to tau pathology. However, previous studies on PET-based brain functional connectivity were mostly conducted at the group level.
Methods: We propose to explore the change of tau deposition from a topological perspective across different neurocognitive levels of dementia both at the individual and group levels. We have developed a method for building subject-level tau networks to investigate the correlations between brain regions. We included 18F-flortaucipir scans of participants diagnosed with cognitive normal (CN), early mild cognitive impairment (EMCI), late mild cognitive impairment (LMCI), and Alzheimer’s disease from the ADNI database. Each scan was used to build a covariance network using SUVR at regions of interest (ROIs). Topological segregation and integration information was extracted and linked to disease progression. We then extracted seven functional clusters from each subject's covariance network and analyzed the connectivity strength for each: medial temporal lobe (MTL), cognitive control, executive control, default mode (DMN), visual (VIS), somatomotor, and language. Clusters with significant change over disease progression were identified. Two converted individuals with insignificant SUVR change were then investigated from a topological perspective.
Results: Overall, topological data analysis outperformed conventional composite SUVR from signature brain regions. Connectivity strength with functional clusters can provide more accurate staging than SUVR (P<0.01), and the longitudinal change is associated better with future disease development at the subject level. MTL, DMN and VIS were identified as hubs vulnerable to early disease conversion before mild cognitive impairment. The main findings from ADNI were replicated in an independent cohort HABS.
Conclusions: The proposed method can potentially monitor Alzheimer’s disease progression at the subject level, in contrast to group-based approaches, which has great potential in monitoring disease progression and response to treatment.
AIM2 is a novel biomarker for predicting prognosis and immunotherapy response of clear cell renal cell carcinoma
Authors: Zhang Zhiyong , Zheng Zaosong , Zeng Xiangbo , Yao Zhihao , Zhu Yuanchao , Yan Guang , Zhou Jiawei , Bahaerguli Miuhuitijiang , Jie Jirong , Xie Kunfeng , Sun Jinwei , Wang Tong , Liang Jing , Liu Lei , Pang Shiyu , Tan Wanlong 
Published: April 2023

Abstract
Background
As an indispensable component of the inflammasome, absent in melanoma 2 (AIM2) plays an essential role in the initiation of the innate immune response, while its effects on clear cell renal cell carcinoma (ccRCC) still remain unclear. In this research, we aimed to evaluate the predictive value of AIM2 on prognosis and immunotherapy effects in patients suffering from ccRCC.
Methods
In this study, genomic and phenotypic data obtained from public databases and ccRCC patient samples from NanFang hospital were collected for exploring the correlation between AIM2 and ccRCC progression. Then we also investigated the association between AIM2 and tumor immune microenvironment of ccRCC patients. Finally, the efficacy of AIM2 was tested to predict the response to immunotherapy of ccRCC patients.
Results
Our study verified that AIM2 was significantly overexpressed in ccRCC tissues compared to adjacent normal tissues with the potential contributing factors including low methylation level and high copy number amplification level of AIM2. AIM2 was an independent prognostic marker of ccRCC patients and significantly associated with higher malignancy. Further analysis suggested that AIM2 was implicated in tumor immune microenvironment (TIME), showing a closely positive association with most inhibitory immune checkpoints. Thus, we further elucidated that ccRCC patients with higher AIM2 mRNA expression levels had more sensitive immunotherapy responses.
Conclusions
This research determined the predictive value of AIM2 in predicting the prognostic and immunotherapy effects of ccRCC patients and revealed its potential to efficiently pick out certain patients that may benefit from cancer immunotherapy.
Intercomparison between METAR- and SYNOP-based fog climatologies
Authors: Yabra Melina Sol , de Elía Ramón , Vidal Luciano , Nicolini Matilde 
Published: April 2023

Abstract
Weather observations and forecasts play a crucial role in the safety and economics of aviation, particularly in the presence of hazards such as fog. Climatological studies of this phenomenon are hence standard information for the aviation industry. Some aeronautical meteorological stations located at airports are also combined with synoptic stations and have to provide local and current weather conditions in two different types of reports: SYNOP for synoptic purposes and METAR for aeronautical purposes. When climatological studies are to be carried out, the question arises of which type of report is more appropriate for the task. In this work, a comparison is made between these two types of hourly meteorological observation reports, with the aim of identifying the conditions under which differences can have a significant impact on the final results. For this purpose, SYNOP and METAR reports for the period 2000-2019 from 13 Argentine airports were selected from diverse databases. A perfect agreement between the two reports is not found, but a varying degree of similarity depends on the variable. A key difference is found in “present weather” mainly due to different reporting rules. In this context, six different selection criteria using visibility, dew point depression, and present weather are used to evaluate the impact of the differences found in the fog climatology. In conclusion, SYNOP data could be used instead of METAR in statistical studies if the need arises, but with the caveat that fog frequency of occurrence should not be interpreted too strictly.
Lorcaserin: Worthy of Further Insights? Results from Recent Research
Authors: Mazza Marianna , Kotzalidis Georgios D. , Marano Giuseppe , De Berardis Domenico , Martinotti Giovanni , Romagnoli Enrico , Biondi-Zoccai Giuseppe , Abbate Antonio , Sani Gabriele 
Published: April 2023

Abstract:
Lorcaserin is a 3-benzazepine that binds 5-HT2C serotonin receptors in the hypothalamus,
where it mediates lack of hunger and/or satiety, and in the ventral tegmental area, the site of origin of
the mesolimbic and mesocortical dopaminergic projections, which mediate pleasure and reward. The
drug has been first developed for the treatment of obesity, where it has shown efficacy, and subsequently
trialed to counter substance use (mostly cocaine, cannabis, opioids, and nicotine) and craving,
but showed inconsistent effects. Since 2020, the US Food and Drug Administration obtained that the
drug was voluntarily withdrawn from the US market on the grounds that its long-term use was found
to be associated with a greater incidence of some types of cancer. Provided it can show to be free from
cancerogenic effects, ongoing research suggests that lorcaserin may have therapeutic potential for a
variety of disorders and conditions beyond obesity. Since 5-HT2C receptors are involved in many diversified
physiological functions (mood, feeding, reproductive behavior, neuronal processes related to
impulsiveness, and modulating reward-related mechanisms) this drug has the potential to treat different
central nervous system conditions, such as depression and schizophrenia.
Experimental Investigation on Mechanical Properties of AA2195-based MMCs for Space Applications
Authors: M Srinivasa Rao , Balaram Padal Dr. K. T. 
Published: April 2023

Background::
The modern Aluminium-Copper-Lithium (Al-Cu-li) based composites have a great demand in aerospace applications due to their lightweight, high strength, high stiffness, and superior mechanical properties.

Objective::
The present study explored the effect of reinforcements like graphite and boron carbide on the mechanical behaviour and microstructure of AA2195 composite

Methods::
The Medium Frequency Induction Furnace (MFIF) was used to fabricate the composites with two factors each at four levels ordered in Taguchi L16 orthogonal array.

Results::
The microstructures revealed that the reinforcements were distributed uniformly throughout the composite. The elemental investigation observed by X-ray diffraction (XRD) revealed that the formed intermetallic compounds (IMCs) helped in refining the microstructure and further increasing the mechanical properties.

Conclusion::
The composite fabricated at 8% boron carbide (B4C) and 6% graphite (Gr) exhibited better ultimate tensile strength, % elongation, and hardness as 190.74 MPa, 0.58, and 87.2VHN, respectively.

conclusion:
The composite fabricated at 8

other:
.
Optimization of Gelling Agent and Sunscreen Activity Test of 4-OH-chalcone Gel by In Vitro Method
Authors: Zulkarnain Abdul Karim , Jumina Jumina , Kharismawati Galih Titiasari , Larasati Fina Anjani 
Published: April 2023

ABSTRACT
The 4-OH-chalcone was suspected to absorb and reduce ultraviolet radiation intensity that exposed to the skin. The 4-OH-chalcone was formulated as gel dosage form using combination of carbopol in content of 0.50-1.00% w/w; propylene glycol in content of 19-20% w/w; and CMC-Na in content of 0.50-1.00% w/w. This study’s purpose was to optimize the three gel bases, evaluate physical properties, physical stability, and activity of 4-OH-chalcone gel as sunscreen by in vitro method. Simplex Lattice Design (SLD) method was used to optimize gel base with Design Expert software version 10.0. Activity of 4-OH-chalcone gel as sunscreen was determined spectrophotometrically by determining Sun Protecting Factor (SPF) value, percent transmission of erythema (% TE), and percent transmission of pigmentation (% TP). Data were analyzed statistically with SPSS Statistic software. Optimum formula of 4-OH-chalcone gel with 0.50% w/w carbopol content, 0.50% w/w carboxymethylcellulose sodium content, and 20,0% w/w propylene glycol content has pH of 5.59, viscosity of 37.4 dPa.s, spreadability of 28.8 cm2, adhesive time of 1.28 seconds, SPF value of 28.65, %, TE value of 16.44%, %TP value of 0.24%, and relatively stable physical properties.
RESTORATIVE JUSTICE SEBAGAI SOLUSI MENGATASI OVERKAPASITAS LAPAS DI INDONESIA
Authors: Triana Ikama Dewi Setia , Erowati Eti Mul 
Published: April 2023

Tujuan dari penelitian ini adalah untuk mengetahui dan menganalisa tentang restorative justice sebagai solusi mengatasi overkapasitas lapas di Indonesia. Penelitian tentang Restorative Justice Sebagai Solusi Mengatasi Overkapasitas Lapas Di Indonesia terdapat Penelitian hukum yang relevan dengan masalah hukum yang diteliti dan mendasarkan analisisnya pada peraturan perundang-undangan yang relevan disebut yuridis normatif. Berdasarkan penelitian yang dilakukan, didapatkan hasil tingkatan restorative justice di Indonesia secara tegas diatur dalam berbagai peraturan perundang-undangan misalnya Undang-Undang Dasar Republik Indonesia Tahun 1945; Undang-Undang Nomor 48 Tahun 2009 Tentang Kekuasaan Kehakiman, Undang-Undang Nomor 14 Tahun 1985 sebagaimana telah diubah oleh UndangUndang Nomor 5 Tahun 2004 sebagaimana telah diubah terakhir dengan Undang-Undang Nomor 3 Tahun 2009 Tentang Mahkamah Agung. Sasaran akhir dari adanya teori peradilan restorative yakni agar lebih sedikit orang yang dipenjara; menghapus stigma atau cap dan merehabilitasi pelaku kejahatan menjadi manusia biasa; penjahat lebih mungkin untuk belajar dari kesalahan mereka dan menghindari membuat kesalahan yang sama lagi, mengurangi beban kerja polisi, jaksa, pusat penahanan, pengadilan dan penjara; Karena korban telah memaafkan pelaku dan segera diberi ganti rugi, menyimpan uang negara tidak menimbulkan keinginan untuk balas dendam; membantu masyarakat memerangi kejahatan dan mengintegrasikan kembali pelaku ke dalam masyarakat.
PENGARUH NASIONALISME, TINGKAT PENDAPATAN, KEPERCAYAAN PADA OTORITAS PAJAK TERHADAP KEPATUHAN MEMBAYAR PAJAK PRIBADI
Authors: Yuli Prastyatini Sri Lestari , Rahmawati Wilis 
Published: April 2023

Individual taxpayer compliance starts from an internal decision whether to comply or not to pay the tax. It turns out that there are still individual taxpayers who do not fulfill their obligations to pay taxes. Seeing this phenomenon, this study aims to determine several factors that can influence compliance to pay personal taxes, namely nationalism, level of income, and trust in the tax authority, especially in the city of Yogyakarta. The data source used is primary data with quantitative methods from 122 respondents. The sampling method was incidental sampling, namely taking samples that were not accidentally encountered by the researcher and suitable for the sample, then processed based on the multiple linear regression analysis methods of the SPSS application version 20. The results of data processing showed that nationalism, income levels, and trust in the tax authorities were factors has a positive effect on compliance with paying personal taxes in the Yogyakarta City area.
Keywords: tax; compliance; influence; quantitative; primary
Kepatuhan wajib pajak orang pribadi dimulai dari keputusan internal apakah bersikap patuh atau tidak untuk membayar pajak tersebut. Ternyata masih ditemukan wajib pajak orang pribadi yang tidak memenuhi kewajibannya membayar pajak. Melihat fenomena tersebut maka penelitian ini bertujuan untuk mengetahui beberapa faktor yang dapat memengaruhi kepatuhan membayar pajak orang pribadi yaitu nasionalisme, tingkat pendapatan, dan kepercayaan pada otoritas pajak khususnya di wilayah Kota Yogyakarta. Sumber data yang digunakan adalah data primer dengan metode kuantitatif dari 122 responden. Metode pengambilan sampel melalui insidental sampling yaitu mengambil sampel yang tidak segaja ditemui oleh peneliti dan cocok untuk sampel kemudian diolah berdasarkan metode analisis regresi linear berganda dari aplikasi SPSS versi 20. Diperoleh hasil olah data yang menunjukkan bahwa faktor nasionalisme, tingkat pendapatan dan kepercayaan pada otoritas pajak berpengaruh positif terhadap kepatuhan membayar pajak orang pribadi di wilayah Kota Yogyakarta.
Kata kunci: pajak; kepatuhan; berpengaruh; kuantitatif; primer
The Hesychast Movement and the Liturgy
Authors: GETCHA Job 
Published: April 2023

This article shows the influence of the Hesychast movement on the liturgy, which led to a major liturgical reform in the Byzantine world. The ideal of praying without ceasing as a fruit of baptism led the hesychasts to consider it as the aim of the life of all Christians, monks and lay people, and to consider the neo-Sabaite Typikon as the most adapted ordo to serve as a school of prayer and to foster vigil and fasting, regarded in the patristic tradition as the main weapons against sin and passions. Conscious that life in Christ was anchored in the sacramental life of the Church, the hesychasts encouraged frequent communion and regarded the sacraments not as acts of individual piety but rather underlined their ecclesial and eschatological dimensions.