bluuebunny/crossref_metadata_2025_split huggingface dataset:
A molecular study on recombinant pullulanase type I from Metabacillus indicus
Authors: Al-Mamoori Zahraa Z. , Embaby Amira M. , Hussein Ahmed , Mahmoud Hoda E. 
Published: April 2023

AbstractDespite the great potential of cold-adapted pullulanase type I in tremendous industrial applications, the majority of commercialized pullulnases type I are of mesophilic and thermophilic origin so far. Hence, the present study underlines cloning, heterologous expression in Escherichia coli, characterization, and in silico structural modeling of Metabacillus indicus open reading frame of cold-adapted pullulanase type I (Pull_Met: 2133 bp & 710 a.a) for the first time ever. The predicted Pull_Met tertiary structure by I-TASSER, was structurally similar to PDB 2E9B pullulanase of Bacillus subtilis. Purified to homogeneity Pull_Met showed specific activity (667.6 U/mg), fold purification (31.7), molecular mass (79.1 kDa), monomeric subunit and Km (2.63 mg/mL) on pullulan. Pull_Met had optimal pH (6.0) and temperature (40 oC). After 10 h pre-incubation at pH 2.6-6.0, Pull_Met maintained 47.12 ± 0.0–35.28 ± 1.64% of its activity. After 120 min pre-incubation at 30 oC, the retained activity was 51.11 ± 0.29%. At 10 mM Mn2+, Na2+, Ca2+, Mg2+, and Cu2+ after 30 min preincubation, retained activity was 155.89 ± 8.97, 134.71 ± 1.82, 97.64 ± 7.06, 92.25 ± 4.18, and 71.28 ± 1.10%, respectively. After 30 min pre-incubation with Tween-80, Tween-20, Triton X-100, and commercially laundry detergents at 0.1% (v/v), the retained activity was 141.15 ± 3.50, 145.45 ± 0.20, 118.12 ± 11.00, and 90%, respectively. Maltotriose was the only end product of pullulan hydrolysis. Synergistic action of CA-AM21 (α-amylase) and Pull_Met on starch liberated 16.51 g reducing sugars /g starch after 1 h at 40 oC. Present data (cold-adeptness, detergent stability, and ability to exhibit starch saccharification of Pull_Met) underpins it as a promising pullulanase type I for industrial exploitation.
Bifunctional role of some biogenic nanoparticles in controlling wilt disease and promoting growth of common bean
Authors: El-Sayed El-Sayed R. , Mohamed Samar S. , Mousa Shaimaa A. , El-Seoud Mohamed A. Abo , Elmehlawy Adel A. , Abdou Dalia A.M. 
Published: April 2023

AbstractIn the present era, nanomaterials are emerging as a powerful tool for management of plant disease and improving crop production to meet the growing global need for food. Thus, this paper was conducted to explore the effectiveness of five different types of nanoparticles (NPs) viz., Co3O4NPs, CuONPs, Fe3O4NPs, NiONPs, and ZnONPs as treatments for Fusarium wilt as well as their role in promoting growth of the common bean plant. The five types of NPs were applied as a treatment for wilt in two ways, therapeutic and protective plans under greenhouse conditions. In vivo experiments showed that all types of NPs significantly increased disease control and diminished the symptoms of Fusarium wilt for both incidence and severity. The recorded values for disease control using the respective NPs during the protective plan were 82.77, 60.17, 49.67, 38.23, and 70.59%. Meanwhile these values were 92.84, 64.67, 51.33, 45.61, 73.84% during the therapeutic plan. Moreover, CuONPs during the protective plan were the best among the five types of NPs employed in terms of wilt disease management. Regarding the use of these NPs as growth promoters, the obtained results confirmed the effectiveness of the five types of NPs in enhancing vegetative growth of the plant under greenhouse conditions, in comparison with control. Among the five NPs, CuONPs improved the plant vegetative growth and particularly increased the content of the photosynthetic pigments; chlorophyll-a (2.96 mg/g), -b (1.93 mg/g), and total carotenoids (1.16 mg/g). These findings suggest the successful and potential exploitation of nanomaterials in agriculture deployed as nano-based products including nano-fungicides and nano-fertilizers. In terms of sustainability, this promising and exceptional multifunctional role of these nanomaterials will surely exert positive impacts on both the environment and sustainable agriculture.
Improving biomass and carbohydrate production of microalgae in the rotating cultivation system on natural carriers
Authors: Mousavian Zahra , Safavi Maliheh , Salehirad Alireza , Azizmohseni Farzaneh , Hadizadeh Mahnaz , Mirdamadi Saeed 
Published: April 2023

AbstractBiofilm-based algal technologies have gained popularity due to higher biomass productivity, efficient harvesting, and water-saving over suspended growth systems. A rotating attached system was designed to assess the biofilm-forming capacity of different isolated microalgal strains from the Persian Gulf. Four microalgal strains, including two Chlorella sp., one Picochlorum sp. and one filamentous cyanobacterium Desmonostoc sp. were cultivated on four carriers: jute, cotton, yarn and nylon. The carriers’ physicochemical surface characteristics and attachment effects, like contact angle, were investigated. The incorporated biomass and exopolysaccharides (EPS) content in the suspended and biofilm system was calculated and compared. The results showed that the cyanobacterium strain had the biofilm formation capability on both jute and cotton in the attached cultivation system. Under the same culture conditions, the biomass productivity on jute and cotton carriers was significantly higher (4.76 and 3.61 g m− 2 respectively) than the growth in aqueous suspension (1.19 g m− 2 d− 1). The greatest incorporated exopolysaccharides amount was observed on jute (43.62 ± 4.47%) and the lowest amount was obtained from the growth on positive charge yarn (18.62 ± 1.88%). This study showed that in comparison with planktonic growth, the colonization of cyanobacterial cells and subsequent production of extracellular matrix and biofilm formation can lead to increased biomass production.
Are ChatGPT and large language models “the answer” to bringing us closer to systematic review automation?
Authors: Qureshi Riaz , Shaughnessy Daniel , Gill Kayden A. R. , Robinson Karen A. , Li Tianjing , Agai Eitan 
Published: April 2023

AbstractIn this commentary, we discuss ChatGPT and our perspectives on its utility to systematic reviews (SRs) through the appropriateness and applicability of its responses to SR related prompts. The advancement of artificial intelligence (AI)-assisted technologies leave many wondering about the current capabilities, limitations, and opportunities for integration AI into scientific endeavors. Large language models (LLM)—such as ChatGPT, designed by OpenAI—have recently gained widespread attention with their ability to respond to various prompts in a natural-sounding way. Systematic reviews (SRs) utilize secondary data and often require many months and substantial financial resources to complete, making them attractive grounds for developing AI-assistive technologies. On February 6, 2023, PICO Portal developers hosted a webinar to explore ChatGPT’s responses to tasks related to SR methodology. Our experience from exploring the responses of ChatGPT suggest that while ChatGPT and LLMs show some promise for aiding in SR-related tasks, the technology is in its infancy and needs much development for such applications. Furthermore, we advise that great caution should be taken by non-content experts in using these tools due to much of the output appearing, at a high level, to be valid, while much is erroneous and in need of active vetting.
Perioperative glycemic management in adults presenting for elective cardiac and non-cardiac surgery
Authors: Sreedharan Roshni , Khanna Sandeep , Shaw Andrew 
Published: April 2023

AbstractPerioperative dysglycemia is associated with adverse outcomes in both cardiac and non-cardiac surgical patients. Hyperglycemia in the perioperative period is associated with an increased risk of postoperative infections, length of stay, and mortality. Hypoglycemia can induce neuronal damage, leading to significant cognitive deficits, as well as death. This review endeavors to summarize existing literature on perioperative dysglycemia and provides updates on pharmacotherapy and management of perioperative hyperglycemia and hypoglycemia in surgical patients.
Loneliness and trust issues reshape mental stress of expatriates during early COVID-19: a structural equation modelling approach
Authors: Billah Md Arif , Akhtar Sharmin , Khan Md. Nuruzzaman 
Published: April 2023

Abstract
Objectives
To explore relationship among perceived stress regarding loneliness, interpersonal trust and institutional trust of expatriates during the early COVID-19 period (from 30th March to 30th May 2020).

Methods
Data from 21,439 expatriates were extracted from COVIDiSTRESS global survey. The outcome variable was perceived stress. The explanatory variables were age, perceived loneliness, trust (interpersonal and institutional). Pairwise correlation, and structural equation modelling were used to determine relationship among outcome and explanatory variables.

Results
The majority of the expatriates were female (73.85%), married (60.20%), had college degree (47.76%), and employed (48.72%). Over 63% of the total expatriates reported that the COVID-19 pandemic changed their lives. The average age of the respondents was 40.4 years (± 13.7), and the average score of perceived stress, loneliness, interpersonal and institutional trust were 25.5, 7.4, 14.2 and 40.4, respectively. We found a moderate correlation of perceived stress with age, perceived loneliness, interpersonal trust and institutional trust (p < 0.001). They were also found moderately related to each other. Structural equation modelling evaluated that a lack of trust can cause loneliness among expatriates, which later lead to perceived stress. Interpersonal trust was more likely to be associated with stress than institutional trust, whereas perceived loneliness mediated between both trusts and perceived stress.

Conclusion
Perceived stress can be reduced through trusting others and alleviating the loneliness. Making strong linkage among migrants as well as between migrants and local community is important to ensure proper mental wellbeing of expatriates.
The influence of weight on psychosocial well-being in diabetes
Authors: Telaak Sydney H. , Costabile Kristi A. , Persky Susan 
Published: April 2023

Abstract
Background
Individuals with diabetes experience a wide variety of psychosocial responses to their illness due, in part, to the nature of type 1 and type 2 diabetes. Variation in patient weight may play a central role in these differences, yet its influence on psychosocial variation is largely unknown. The current study investigates the relationship between patients’ perceived weight status and aspects of psychosocial well-being among individuals with type 1 diabetes (T1D) and type 2 diabetes (T2D).

Methods
Individuals who were diagnosed with type 1 or type 2 diabetes were assessed via an online survey from the Diabetes, Identity, Attributions, and Health Study. Participants were categorized into a lower v. higher weight status group based on their self-reported perceived weight. Analyses of covariance were conducted to assess differences in measures of disease onset blame, diabetes stigma, and identity concerns among diabetes type and perceived weight status. Covariates included in our models were gender, age, education, and time since diagnosis. Bonferroni correction was used for post-hoc tests to assess any significant interactions found in our models.

Results
Findings indicated that weight moderates multiple psychosocial outcomes pertinent to illness experience. Those with T2D and lower weight blamed themselves less for their disease onset, while those with higher weight felt blamed more for their disease onset by others, regardless of diabetes type. Individuals with T1D and higher weight were more frequently and more concerned about being mistaken for having the other disease type (i.e., T2D) compared to those with lower weight.

Conclusions
Weight is a key influence on the psychosocial outcomes for people with diabetes, but it operates differently in type 1 versus type 2 diabetes. By further examining the unique interaction between disease type and weight status we may be able to improve psychological well-being among affected individuals of all sizes.
Transfer learning approach based on satellite image time series for the crop classification problem
Authors: Antonijević Ognjen , Jelić Slobodan , Bajat Branislav , Kilibarda Milan 
Published: April 2023

AbstractThis paper presents a transfer learning approach to the crop classification problem based on time series of images from the Sentinel-2 dataset labeled for two regions: Brittany (France) and Vojvodina (Serbia). During preprocessing, cloudy images are removed from the input data, the time series are interpolated over the time dimension, and additional remote sensing indices are calculated. We chose TransformerEncoder as the base model for knowledge transfer from source to target domain with French and Serbian data, respectively. Even more, the accuracy of the base model with the preprocessing step is improved by 2% when trained and evaluated on the French dataset. The transfer learning approach with fine-tuning of the pre-trained weights on the French dataset outperformed all other methods in terms of overall accuracy 0.94 and mean class recall 0.907 on the Serbian dataset. Our partially fine-tuned model improved recall of crop types that were poorly classified by the base model. In the case of sugar beet, class recall is improved by 85.71%.
Outcomes of a food voucher program and factors associated with the recovery rate of children with moderate acute malnutrition in Far North Cameroon
Authors: Teta Ismael , Foudjo Brice Ulrich Saha , Nielsen Jennifer N. , Oben Julius , Nguefack-Tsague Georges , Ntentie Françoise Raissa , Cakir Volkan , Klemm Rolf , Kang Yunhee 
Published: April 2023

Abstract
Background
Research on moderate acute malnutrition (MAM) is limited, despite its high prevalence. This study examined outcomes of bi-weekly locally available foods provided via a food voucher program (FVP) on nutritional recovery [mid-upper arm circumference (MUAC) ≥ 125 mm] from MAM (defined as MUAC between 115 and 124 mm) and identified the factors associated with recovery rate in Kaélé health district, Far North Region of Cameroon.

Methods
This was a prospective study with 474 MAM children aged 6–59 months. Food voucher distribution and MUAC screening were conducted at 6 bi-weekly visits or until the child was recovered. Time to recovery was evaluated with multivariate Cox proportional regression hazard models with associations quantified using adjusted hazard ratio (aHR). The trend for MUAC, including its determinants, was examined with multivariate linear mixed effect models.

Results
The recovery rate was 78.3% by 6 weeks after the first food basket; 3.4% remained MAM, and 5.9% were transferred for treatment for severe acute malnutrition (SAM defined as MUAC < 115 mm). Boys were 34% more likely to recover from MAM than girls [aHR = 1.34, 95%CI (1.09, 1.67)]. Children aged 24–53 months were 30% more likely to recover than those aged 6–11 months [aHR = 1.30, 95%CI (0.99, 1.70)]. A one unit increase in weight-for-height Z-score (WHZ) was associated with 1.89-fold greater likelihood of recovery [aHR = 1.89, 95%CI (1.66, 2.14)]. Male children had on average 1.82 mm greater increase in MUAC than female children (p < 0.001). One unit increase in WHZ was associated with 3.42 mm increase in MUAC (p = 0.025). Children aged 12–23 and 24–53 months had 1.03 mm and 2.44 mm, respectively, greater increase in MUAC over the program than children aged 6–11 months (all p < 0.01).

Conclusion
The recovery rate of MAM children treated with the FVP met the Sphere standards for targeted supplementary feeding programs (> 75%). Child’s WHZ, gender and age were significant factors associated with MUAC increase and recovery from MAM in the FVP. These findings indicate the FVP approach shows promise as an effective alternative treatment for MAM with consideration of associated factors and merits further evaluation.
Anatomical and histological study on the tongue of two different species Ptychadena mascareniensis and Hemidactylus turcicus from the Egyptian environment
Authors: Abdel Moghith Zainab SH. , Salama Maiada M. , Hassan Basma N. , Abdelkader Ebrahim Y. , El-Habit Ola H. 
Published: April 2023

Abstract
Background
The tongue plays definite and important role in simplifying food intake and also, exhibits significant morphological distinctions to acclimatize the environment. This study aimed to investigate the variations in gross and microscopic anatomy of the tongue in two tetrapod classes Ptychadena mascareniensis from class: Amphibia and Hemidactylus turcicus from class: Reptilia to correlate the structure and functions of the tongue in relations to the habitat and feeding mechanisms.

Results
All the modifications perceived in the tongues of the premeditated animals explained the relationship between adaptation of each animal to its feeding habit and habitat. Amphibians which live in and nearby freshwater showed no keratinization in their lingual epithelium. However, reptiles, which live in terrestrial habitats, have shown keratinization and ideal forms of lingual papillae which considered the most remarkable feature of the histological structures of the reptilian tongues and reflect their adaptations to the dry habitat in which they live.

Conclusion
There were some variations reported in histological structure, gross anatomy and ultrastructure morphology of the tongue from the two tetrapod classes, the Amphibia (Ptychadena mascareniensis) and Reptilia (Hemidactylus turcicus). The present study is the first one in Egypt that deals with the tongue structures and functions in one of the amphibians Ptychadena mascareniensis. Concerning the lizard Hemidactylus turcicus.
Effect of HCV treatment with DAAs on serum intestinal fatty acid binding protein (I-FABP) as a marker of intestinal permeability in HCV/HIV co-infected patients
Authors: Abdelhaleem Hanan , Khairy Marwa , Abdo Mahmoud , Cordie Ahmed , Elsharkawy Marwa , Alem Shereen Abdel , Elsharkawy Aisha , Al sehemy Lamiaa , Esmat Gamal 
Published: April 2023

Abstract

Background
HCV and HIV co-infected patients develop cirrhosis more rapidly than HCV mono-infection. Intestinal injury and microbial translocation are postulated mechanisms for the rapid progression of cirrhosis.

Aim
Study the effect of HCV treatment with DAAs on serum intestinal fatty acid binding protein (I-FABP) as a marker of intestinal injury in HCV/HIV co-infected patients and its relation to hepatic fibrosis. Comparing the level of I-FABP in HCV mono-infection and HCV/HIV co-infection was a secondary aim.

Methods
I-FABP levels were measured in 50 non-cirrhotic HCV/HIV co-infected patients pre- and post-HCV treatment (SVR 12) (25 patients were HIV treatment naive, and 25 patients were on HAART) and in 25 chronic HCV patients as a control group. Hepatic fibrosis was assessed by FIB4 score, APRI score, and transient elastography.

Results
HCV/HIV co-infected patients had significantly higher levels of I-FABP compared to the HCV-mono-infected patients (P = 0.001). After HCV treatment in HCV/HIV co-infected patients, I-FABP level was significantly elevated (P < 0.001) and was positively correlated with baseline FIB4 values and serum ALT levels (r = 0.283, P-value = 0.047) and (r = 0.340, P-value = 0.016), respectively.

Conclusion
HCV/HIV co-infection is associated with significantly higher intestinal injury and subsequent hepatic fibrosis than HCV mono-infection. HIV infection is associated with intestinal epithelial injury and microbial translocation and may play a role in the persistence of systemic inflammation after HCV eradication.
The effect of investment in the brand value chain on profitability and market value of the firm: lesson of success taken from Amazon
Authors: Elsayed Raghda Abdellatif Abdelkhalik 
Published: April 2023

AbstractThis study aims to contribute to the existing business strategy literature by exploring how brand-building activities contribute to the firm's value by analyzing the direct and indirect effect of investment in the brand value chain on brand resonance, profitability, and market value. This study is based on a random sample of 237 Certified European Financial Analysts and a questionnaire was employed. Harman’s single-factor test was conducted to define the presence of common method bias. Hayes Process Macro Model 5 was used to check the multiple mediation processes and indirect effects between the study's variables. The study employs the financial statements of Amazon.com, Inc. from 2010 to 2021 which has been analyzed under the standard multiple regression model. The study results reveal that Harman’s single-factor test extracts 10.397% of the total variance. On the other hand, there are significant positive relationships between the effect of investment in the brand value chain on brand resonance, profitability, and market value. The empirical evidence of this study provides an opportunity to learn about the effect of the investment in the brand value chain leads to increase brand equity which builds up in the customers' mindset and then transforms to market performance and profitability. The findings of this study can be a useful reference for future studies in academic research related to strategic thinking to maximize profitability and market value through the brand value chain.
Isometric chin tuck exercise with and without scapular postural correction for patients with chronic neck pain: a longitudinal study
Authors: Yong Joon Hyoung , Moon Il Young , Lim Jin Seok , Yi Chung Hwi 
Published: April 2023

Background/AimsChronic neck pain is common in individuals with poor posture and motor control impairments. There has been increased interest in scapular postural correction in conjunction with the isometric chin tuck exercise. The aim of this study was to investigate the clinical efficacy of scapular postural correction while performing isometric chin tuck exercises for the improvement of neck pain, neck and shoulder alignment and superficial neck muscle activity in patients with chronic neck pain.MethodsA total of 32 participants with chronic, non-specific neck pain were randomly divided into two groups (groups A and B, both n=16), who performed isometric chin tuck exercises alone or isometric chin tuck with scapular postural correction respectively. The participants were instructed to perform five sets of the exercise with 10 repetitions for 6 weeks. Measurements were taken pre- and post-intervention to determine Neck Disability Index scores, postural parameters (head, cervical and shoulder angle) and superficial cervical muscle activity (sternocleidomastoid, anterior scalene, splenius capitis). Analysis of covariance was used to compare the post-intervention scores.ResultsSignificant differences were observed between groups A and B in the cervical angle (F1,29=4.538, P=0.042) and shoulder angle (F1,29=8.357, P=0.007), when compared to baseline values. Both group A (t=12.097, P<0.001) and group B (t=8.032, P<0.001) showed a significant reduction in Neck Disability Index scores compared to baseline scores. Both group A and group B showed a significant decrease in sternocleidomastoid (group A: t=6.637, P<0.001; group B: t=10.143, P<0.001), anterior scalene (group A, t=5.484, P<0.001; group B, t=8.063, P<0.001), and splenius capitis (group A, t=8.591, P<0.001; group B, t=8.258, P<0.001) muscle activity compared to baseline values.ConclusionsIsometric chin tuck alone and isometric chin tuck combined with scapular postural correction both showed significant reductions in neck pain and superficial neck muscle activity. Additional scapular postural correction training significantly increased the cervical angle and decreased the shoulder angle.