bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asymmetry of sleep electrophysiological markers in patients with focal epilepsy
Authors: Sheybani Laurent , Mégevand Pierre , Roehri Nicolas , Spinelli Laurent , Kleinschmidt Andreas , van Mierlo Pieter , Seeck Margitta , Vulliémoz Serge 
Published: May 2023

Abstract
Sleep can modulate epileptic activities, but our knowledge of sleep perturbation by epilepsy remains sparse. Interestingly, epilepsy and sleep both present with defining electrophysiological features in the form of specific graphoelements on EEG. This raises the possibility to identify, within ongoing EEG activity, how epilepsy impacts and disrupts sleep. Here, we asked whether the presence of a lateralized epileptic focus interferes with the expression of the dominant electrophysiological hallmarks of sleep: slow oscillations, slow waves and spindles. To this aim, we conducted a cross-sectional study and analysed sleep recordings with surface EEG from 69 patients with focal epilepsy (age range at EEG: 17–61 years, 29 females, 34 left focal epilepsy). Comparing patients with left and right focal epilepsy, we assessed inter-hemispheric asymmetry of sleep slow oscillations power (delta range, 0.5–4 Hz); sleep slow wave density; amplitude, duration and slope; and spindle density, amplitude, duration as well as locking to slow oscillations. We found significantly different asymmetries in slow oscillation power (P &lt; 0.01); slow wave amplitude (P &lt; 0.05) and slope (P &lt; 0.01); and spindle density (P &lt; 0.0001) and amplitude (P &lt; 0.05). To confirm that these population-based differences reflect actual patient-by-patient differences, we then tested whether asymmetry of sleep features can classify laterality of the epileptic focus using a decision tree and a 5-fold cross-validation. We show that classification accuracy is above chance level (accuracy of 65%, standard deviation: 5%) and significantly outperforms a classification based on a randomization of epileptic lateralization (randomization data accuracy: 50%, standard deviation 7%, unpaired t-test, P &lt; 0.0001). Importantly, we show that classification of epileptic lateralization by the canonical epileptic biomarker, i.e. interictal epileptiform discharges, improves slightly but significantly when combined with electrophysiological hallmarks of physiological sleep (from 75% to 77%, P &lt; 0.0001, one-way ANOVA + Sidak’s multiple comparisons test). Together, we establish that epilepsy is associated with inter-hemispheric perturbation of sleep-related activities and provide an in-depth multi-dimensional profile of the main sleep electrophysiological signatures in a large cohort of patients with focal epilepsy. We provide converging evidence that the underlying epileptic process interacts with the expression of sleep markers, in addition to triggering well-known pathological activities, such as interictal epileptiform discharges.
miR-424-3p promotes metastasis of hepatocellular carcinoma via targeting the SRF-STAT1/2 axis
Authors: Feng Lan , Chen Xi , Li Peiyao , Li Yuanfeng , Zhai Yun , Liu Xinyi , Jin Qian , Zhang Hongxing , Yu Chaohui , Xing Baocai , Cui Ying , Cao Pengbo , Zhou Gangqiao 
Published: May 2023

Abstract
Although emerging evidence has established the roles of miRNAs in hepatocellular carcinoma (HCC), the global functional implication of miRNAs in this malignancy remains largely uncharacterized. Here, we aim to systematically identify novel miRNAs involved in HCC and clarify the function and mechanism of specific novel candidate miRNA(s) in this malignancy. Through an integrative omics approach, we identified ten HCC-associated functional modules and a collection of candidate miRNAs. Among them, we demonstrated that miR-424-3p, exhibiting strong associations with extracellular matrix (ECM), promotes HCC cells migration and invasion in vitro and facilitates HCC metastasis in vivo. We further demonstrated that SRF is a direct functional target of miR-424-3p, and is required for the oncogenic activity of miR-424-3p. Finally, we found that miR-424-3p reduces the interferon pathway by attenuating the transactivation of SRF on STAT1/2 and IRF9 genes, which in turn enhances the matrix metalloproteinases (MMPs)-mediated ECM remodeling. This study provides comprehensive functional relevance of miRNAs in HCC by an integrative omics analysis, and further clarifies that miR-424-3p in ECM functional module plays an oncogenic role via reducing the SRF-STAT1/2 axis in this malignancy.
Rituximab in the treatment of primary FSGS: time for its use in routine clinical practice?
Authors: Morris Adam D , Floyd Lauren , Woywodt Alexander , Dhaygude Ajay 
Published: May 2023

ABSTRACT
Focal segmental glomerulosclerosis (FSGS) is a common cause of nephrotic syndrome and whilst advances have been made in the pathophysiology, diagnostics and management of other podocytopathies, primary FSGS remains the most elusive. It has been assumed for a long time that a circulatory permeability factor exists that mediates podocyte injury, and the potential for autoantibody-mediated disease therefore raises the question as to whether patients may benefit from targeted B-cell therapy with rituximab. The prospective case series of seven patients by Roccatello et al. adds to the limited but growing evidence suggesting that B-cell depletion therapy can be safe and effective in the treatment of primary FSGS. In this editorial we explore the available evidence that suggests how and in whom rituximab may play a role in the management of primary FSGS, as well as the limitations and other potential future treatments. Further research and randomized controlled trials are needed to include larger numbers of patients, feature genetic screening and incorporate data on B-cell kinetics as a potential guide for dosing and frequency of rituximab.
An Examination of School District Reopening Plans for Mental Health Supports amid COVID-19 School Reopening
Authors: Childs Tasha M , Iachini Aidyn L 
Published: May 2023

Abstract
The purpose of this exploratory qualitative study was to understand the types of mental health supports described in school district reopening plans in one southeastern state, and to understand school social workers’ involvement in developing these plans during the first full academic year of the COVID-19 pandemic. Publicly accessible school district reopening plans were collected from the 80 school districts in South Carolina, and 15 school social workers participated in virtual interviews. Out of the 80 school districts, 67 had reopening plans, and of those, only 43 percent mentioned mental health services and supports. Most school district reopening plans described Tier 1 universal prevention services and supports and a process for mental health assessment, identification, and referral. Fewer included Tier 2 early intervention and Tier 3 targeted and intensive services. Of the school social workers interviewed, only four were involved in helping to develop their school district reopening plans, and when involved, advocated for mental health services and supports for their students. The article concludes with a discussion of the study's implications for school social work practice.
Mid-term outcomes following the Hemi-Commando procedure for complex infective endocarditis involving the aortomitral junction
Authors: Marin-Cuartas Mateo , De La Cuesta Manuela , Davierwala Piroze M , Kang Jagdip , Stöger Guillermo , Misfeld Martin , Kiefer Philipp , Leontyev Sergey , Verevkin Alexander , Pfanmüller Bettina , Saaed Diyar , Borger Michael A , Noack Thilo 
Published: May 2023

Abstract

OBJECTIVES
Perivalvular abscesses with destruction of the aortomitral junction (AMJ) are a severe complication of infective endocarditis (IE) and are associated with high mortality and complex management. The Hemi-Commando procedure is a mitral valve-sparing alternative to the Commando procedure in suitable patients with complex IE and paravalvular destruction. This study reviews the mid-term outcomes in patients undergoing the Hemi-Commando procedure for treating IE with destruction of the AMJ.

METHODS
The clinical outcomes of patients with IE and AMJ involvement who underwent the Hemi-Commando procedure between 2015 and 2021 at the Leipzig Heart Center were retrospectively analysed. Primary outcomes were 30-day mortality and 1-year survival. Secondary outcome was 1-year freedom from reoperation.

RESULTS
A total of 22 patients underwent the Hemi-Commando procedure during the study period. The patients’ mean age was 59.8 ± 18.3 years. The study population was predominantly male (86.4%). Preoperative sepsis was present in 6 (27.3%) patients, and the median EuroSCORE II was 28.5%. Almost two-thirds (N = 14; 63.6%) of the patients presented with native IE. Streptococci were the most common pathogens (N = 8; 36.4%). Paravalvular abscess was found intraoperatively in 16 (72.7%) patients. The 30-day mortality was 13.6%. The estimated 1- and 3-year survival rates were 77.5% and 66.4%, respectively. The estimated freedom from reoperation at 1 and 3 years was 92.3%.

CONCLUSIONS
The Hemi-Commando procedure offers an acceptable mid-term survival chance with low reoperation rates and is, therefore, a reasonable mitral valve-sparing alternative to the Commando procedure in suitable patients with extensive IE and perivalvular involvement.
Risk factors for gingival recessions after orthodontic treatment: a systematic review
Authors: Cadenas de Llano-Pérula Maria , Castro Ana Belén , Danneels Margaux , Schelfhout Alix , Teughels Wim , Willems Guy 
Published: May 2023

Summary

Background/objectives
To systematically review the available evidence concerning the risk factors for gingival recessions (GR) after orthodontic treatment (OT).

Data collection and analysis
Data was obtained and collected by systematically searching 3 data bases: Pubmed, EMBASE, and Web of Science until 20 April 2023. Controlled trials, cohort, case-control or cross-sectional studies describing GR or clinical crown height (CCH) after OT were included. The risk of bias in the selected studies was evaluated with the methodological index for non-randomized studies.

Results
Forty-eight articles were included, investigating the following six risk factors for GR: 1. OT (n = 21), 2. Type of orthodontic intervention (n = 32), 3. Patient’s baseline occlusal and skeletal characteristics (n = 14), 4. Mucogingival characteristics (n = 10), 5. Oral hygiene (n = 9), and 6. Others (n = 12). Significantly higher prevalence, severity and extent of GR were found in orthodontic patients by 10/15, 4/10, and 2/2 articles respectively. 10/16 articles reported significantly more GR and increased CCH in patients where orthodontic incisor proclination was performed. The evidence surrounding maxillary expansion and orthodontic retention was too heterogeneous to allow for? definitive conclusions. Pre-treatment angle classification, ANB, overjet, overbite, arch width and mandibular divergence were found not to be associated with GR (9/14), while pre-treatment crossbite, symphysis height and width were (5/7 studies). A thin gingival biotype, presence of previous GR, baseline width of keratinized gingiva and facial gingival margin thickness were correlated with increased risk of GR after OT by nine articles, while pocket depth was not. Oral hygiene, sex, treatment duration, and oral piercings were found not to be linked with GR in orthodontic patients, while GR was reported to increase with age in orthodontic patients by 50 per cent of the articles investigating this factor. The mean risk of bias for comparative and not comparative studies was 14.17/24 and 9.12/16.

Limitations
The selected studies were quite heterogeneous regarding study settings, variables reported and included very limited sample sizes.

Conclusion
Although studies regarding the risk factors for GR are relatively abundant, they are very heterogeneous concerning design, studied factors, methodology and reporting, which often leads to contradictory results. Uniform reporting guidelines are urgently needed for future research.

PROSPERO Registration
CRD42020181661.

Funding
This research received no funding.
Leaf litter combustion properties of Central European tree species
Authors: Ewald Michael , Labenski Pia , Westphal Erik , Metzsch-Zilligen Elke , Großhauser Michael , Fassnacht Fabian Ewald 
Published: May 2023

Abstract
Temperate forests of Central Europe are exposed to increasing fire risk. However, little is known about combustion properties of leaf litter, which plays an important role in the spread of surface fires. We used cone calorimetry to compare combustion properties of leaf litter samples from seven common tree species of Central European forests by reconstructing a litter layer of original depth in sample holders with a size of 10 cm × 10 cm. In addition to mono-specific leaf litter beds, combustion experiments included mixtures of different litter types, mixtures of litter and bryophytes and one mixture of litter and fine woody debris, totalling to 13 different setups (i.e. litter types). Recorded combustion properties included ignitability, flaming duration and heat release. Differences in combustion properties were analysed using analyses of variance followed by pairwise post-hoc tests. Combustion properties mainly differed between different litter types (broadleaf, pine needle, short needle). Highest total and peak heat release were observed for Scots pine (Pinus sylvestris), while peak heat release rates showed only minor differences for litter of the remaining species. Broadleaf litter was characterized by highest ignitability. For short-needle litter, we observed long flaming duration and incomplete combustion, resulting in the lowest total heat release on a sample mass basis. For litter mixtures of pine and broadleaf litter, we observed lower peak heat release rates in comparison to mono-specific pine litter. Mosses reduced peak heat release rates and increased the proportion of unburned biomass. However, the magnitude of this effect differed between bryophyte species included in the mixtures. The addition of fine woody debris strongly increased total heat release, highlighting the importance of fine woody fuels for fire behaviour. The results of this study provide valuable baseline information on combustion behaviour of leaf litter from Central European forests. Due to the limitations of laboratory combustion experiments to reproduce conditions of real forest fires, there is a need for future field studies investigating fire behaviour under natural conditions.
The Role of Repetitive Sequences in Repatterning of Major Ribosomal DNA Clusters in Lepidoptera
Authors: Dalíková Martina , Provazníková Irena , Provazník Jan , Grof-Tisza Patrick , Pepi Adam , Nguyen Petr 
Published: May 2023

Abstract
Genes for major ribosomal RNAs (rDNA) are present in multiple copies mainly organized in tandem arrays. The number and position of rDNA loci can change dynamically and their repatterning is presumably driven by other repetitive sequences. We explored a peculiar rDNA organization in several representatives of Lepidoptera with either extremely large or numerous rDNA clusters. We combined molecular cytogenetics with analyses of second- and third-generation sequencing data to show that rDNA spreads as a transcription unit and reveal association between rDNA and various repeats. Furthermore, we performed comparative long read analyses among the species with derived rDNA distribution and moths with a single rDNA locus, which is considered ancestral. Our results suggest that satellite arrays, rather than mobile elements, facilitate homology-mediated spread of rDNA via either integration of extrachromosomal rDNA circles or ectopic recombination. The latter arguably better explains preferential spread of rDNA into terminal regions of lepidopteran chromosomes as efficiency of ectopic recombination depends on the proximity of homologous sequences to telomeres.
Rapid Evolution of the Embryonically Expressed Homeobox GeneLEUTXwithin Primates
Authors: Lewin Thomas D , Blagrove Josephine R , Holland Peter W H 
Published: May 2023

AbstractLEUTX is a homeodomain transcription factor expressed in the very early embryo with a function around embryonic genome activation. The LEUTX gene is found only in eutherian mammals including humans but, unlike the majority of homeobox genes, the encoded amino acid sequence is very different between divergent mammalian species. However, whether dynamic evolution has also occurred between closely related mammalian species remains unclear. In this work, we perform a comparative genomics study of LEUTX within the primates, revealing dramatic evolutionary sequence change between closely related species. Positive selection has acted on sites in the LEUTX protein, including six sites within the homeodomain; this suggests that selection has driven changes in the set of downstream targets. Transfection into cell culture followed by transcriptomic analysis reveals small functional differences between human and marmoset LEUTX, suggesting rapid sequence evolution has fine-tuned the role of this homeodomain protein within the primates.
Reticulons 1 and 3 are essential for axonal growth and synaptic maintenance associated with intellectual development
Authors: Zhou John , Shi Qi , Ge Ying Y , He Wanxia , Hu Xiangyou , Xia Weiming , Yan Riqiang 
Published: May 2023

Abstract
Reticulon (RTN) proteins are a family of proteins biochemically identified for shaping tubular endoplasmic reticulum, a subcellular structure important for vesicular transport and cell-to-cell communication. In our recent study of mice with knockout of both reticulon 1 (Rtn1) and Rtn3, we discovered that Rtn1−/−;Rtn3−/− (brief as R1R3dKO) mice exhibited neonatal lethality, despite the fact that mice deficient in either RTN1 or RTN3 alone exhibit no discernible phenotypes. This has been the first case to find early lethality in animals with deletion of partial members of RTN proteins. The complete penetrance for neonatal lethality can be attributed to multiple defects including the impaired neuromuscular junction found in the diaphragm. We also observed significantly impaired axonal growth in a regional-specific manner, detected by immunohistochemical staining with antibodies to neurofilament light chain and neurofilament medium chain. Ultrastructural examination by electron microscopy revealed a significant reduction in synaptic active zone length in the hippocampus. Mechanistic exploration by unbiased proteomic assays revealed reduction of proteins such as FMR1, Staufen2, Cyfip1, Cullin-4B and PDE2a, which are known components in the fragile X mental retardation pathway. Together, our results reveal that RTN1 and RTN3 are required to orchestrate neurofilament organization and intact synaptic structure of the central nervous system.
A Hölder-Type Inequality for the C0 Distance and Anosov–Katok Pseudo-Rotations
Authors: Joksimović Dušan , Seyfaddini Sobhan 
Published: May 2023

Abstract
We prove a Hölder-type inequality for Hamiltonian diffeomorphisms relating the $C^0$ norm, the $C^0$ norm of the derivative, and the Hofer/spectral norm. We obtain as a consequence that sufficiently fast convergence in Hofer/spectral metric forces $C^0$ convergence. The second theme of our paper is the study of pseudo-rotations that arise from the Anosov–Katok method. As an application of our Hölder-type inequality, we prove a $C^0$ rigidity result for such pseudo-rotations.
Spectral Gap Characterizations of Property (T) for II1 Factors
Authors: Tan Hui 
Published: May 2023

Abstract
For II$_1$ factors, we show that property (T) is equivalent to having weak spectral gap in any inclusion into a larger tracial von Neumann algebra. We also show that not having non-zero almost central vectors in weakly mixing bimodules characterizes property (T) for II$_1$ factors, which allows us to obtain a stronger characterization of property (T) where only weak spectral gap in any irreducible inclusion is required.
Why delegate to the IMF? Congressional preference and blame avoidance
Authors: Fujita Masafumi 
Published: May 2023

AbstractWhy has the United States delegated most of its crisis lending to the International Monetary Fund (IMF) in recent years, although it provided large-scale bilateral bailouts to strategically important countries until the mid-1990s? Previous research on the choice of bailout strategy has failed to explain this important change, and a major problem with such research is that it has focused on executive branch preferences, overlooking those of the legislative branch. The legislature can significantly influence the choice of bailout policies, and existing research also implies that the US Congress has steered the recent change. This article hypothesizes that, caught in a dilemma between the need for bailouts and voters’ opposition caused by widening inequality, Congress delegated bailouts to the IMF for blame avoidance. To test this hypothesis, the study conducts a statistical analysis of the IMF’s capital increase votes and case analyses of the Mexican and Asian crises.
Non-randomized evaluation of hospitalization after a prescription for nirmatrelvir/ritonavir versus molnupiravir in high-risk COVID-19 outpatients
Authors: Cowman Kelsie , Miller Alexander , Guo Yi , Chang Mei H , McSweeney Terrence , Bao Hongkai , Simpson Roxanne , Braithwaite Claire , Sunu Evans , Ros Theary , Rodriguez Maria , Laboy Eric , Bard Linda , Alsina Leslie , Cintron Angelica , Andrews Erin , Nori Priya 
Published: May 2023

Abstract

Objectives
To assess and compare subsequent hospital admissions within 30 days for patients after receiving a prescription for either oral nirmatrelvir/ritonavir or oral molnupiravir.

Methods
We conducted a retrospective review of 3207 high-risk, non-hospitalized adult COVID-19 patients who received a prescription for molnupiravir (n = 209) or nirmatrelvir/ritonavir (n = 2998) at an academic medical centre in New York City from April to December 2022. Variables including age, vaccination status, high-risk conditions and demographic factors were pulled from the electronic medical record. We used multivariable logistic regression to adjust for potential confounding variables.

Results
All-cause 30 day hospitalization was not significantly different between patients who received nirmatrelvir/ritonavir compared with molnupiravir (1.4% versus 1.9%, P value = 0.55). The association between COVID-related hospitalization and medication was also not significant (0.7%versus 0.5%, P value = 0.99). Patients who received molnupiravir were more likely to have more underlying high-risk conditions. After adjusting for potential confounders, the odds of all-cause hospitalizations were not significantly different between patients who received nirmatrelvir/ritonavir compared with molnupiravir (OR = 1.16, 95% CI: 0.4–3.3, P value = 0.79).

Conclusions
These data provide additional evidence to support molnupiravir as a suitable alternative when other COVID-19 antivirals cannot be given.