bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding HOXA-AS3 contributes to osteosarcoma progression through the miR-1286/TEAD1 axis
Authors: Xiao Xiangjun , Liu Mingjiang , Xie Songlin , Liu Changxiong , Huang Xinfeng , Huang Xiongjie 
Published: September 2023

AbstractLong non-coding RNA (lncRNA) HOXA cluster antisense RNA 3 (HOXA-AS3) regulates the progression of several types of human malignancy. However, the role and potential mechanism of HOXA-AS3 in osteosarcoma (OS) remain unknown. In this study, upregulation of HOXA-AS3 was observed in OS tissues and cell lines and associated with poor clinical outcomes. Silencing of HOXA-AS3 significantly inhibited the proliferation, migration and invasion of OS cells in vitro and suppressed the tumorigenesis of OS cells in vivo. Furthermore, knockdown of HOXA-AS3 inhibited the proliferation and migration of human umbilical vein endothelial cells (HUVECs) and epithelial-to-mesenchymal transition (EMT) in OS. Further investigation of this mechanism revealed that HOXA-AS3 could directly upregulate the expression of TEAD1 via its competing endogenous RNA (ceRNA) activity on miR-1286. This study clarified the oncogenic roles of the HOXA-AS3/miR-1286/TEAD1 axis in OS progression, suggesting a novel therapeutic target for OS.
Destruction, disruption and disaster: Sudan’s health system amidst armed conflict
Authors: Dafallah Alaa , Elmahi Osman K. O. , Ibrahim Maisoon Elbukhari , Elsheikh Rania Elfatih , Blanchet Karl 
Published: September 2023

AbstractThe ongoing armed conflict in Sudan has resulted in a deepening humanitarian crisis with significant implications for the country's health system, threatening its collapse. This article examines the destruction, disruption, and disastrous consequences inflicted upon Sudan's health system. The conflict has led to the severe compromise of healthcare facilities, with only one-third of hospitals in conflict zones operational. Artillery attacks, forced militarization, power outages, and shortages of medical supplies and personnel have further crippled the health system. The exodus of health workers and escalating violence have exacerbated the crisis. Disrupted service delivery has resulted in the interruption of essential health services, including obstetric care, emergency services, and dialysis. Financial losses to the health system are estimated at $700 million, impacting an already underfunded sector. We identify that in addition to restoration of peace and mobilization of urgent aid, immediate prioritization of the reconstruction of the health system is crucial to mitigate the long-term consequences of the war. Rebuilding a resilient health system is sine qua non for Sudan's progress towards universal health.
Synchronic development of hydrocele and groin hernia during pregnancy: a case report
Authors: Rasmussen Lise , Farooqui Waqas , Ries Annedorte , Stadeager Morten Willer 
Published: September 2023

Abstract
Introduction
Hydroceles of the canal of Nuck are rare, and have not been described in relation to pregnancy.

Case presentation
A 34-year-old Caucasian female patient had bilateral groin swelling debuted during her pregnancy. A preoperative magnetic resonance imaging scan found bilateral hydroceles of the canal of Nuck. Operative findings and histological examinations revealed a left-sided inguinal hernia and a right-sided hydrocele. The patient was discharged well and without signs of postoperative complications or hernia recurrence.

Discussion
In this case, a hydrocele and a hernia sac were morphologically identical in terms of preoperative appearance and development. Given the morphological correlation, it was surprising to find different operative findings confirmed by the histopathological examination.

Conclusion
This is the first ever report of the synchronic development of two morphologically identical cystic processes, with one being a hydrocele and the other a hernia sac. In addition, the hydrocele developed during pregnancy, making this case even more unique.
Human osteoarthritic articular cartilage stem cells suppress osteoclasts and improve subchondral bone remodeling in experimental knee osteoarthritis partially by releasing TNFAIP3
Authors: Li Zhi-Ling , Li Xiao-Tong , Hao Rui-Cong , Wang Fei-Yan , Wang Yu-Xing , Zhao Zhi-Dong , Li Pei-Lin , Yin Bo-Feng , Mao Ning , Ding Li , Zhu Heng 
Published: September 2023

Abstract
Background
Though articular cartilage stem cell (ACSC)-based therapies have been demonstrated to be a promising option in the treatment of diseased joints, the wide variety of cell isolation, the unknown therapeutic targets, and the incomplete understanding of the interactions of ACSCs with diseased microenvironments have limited the applications of ACSCs.

Methods
In this study, the human ACSCs have been isolated from osteoarthritic articular cartilage by advantage of selection of anatomical location, the migratory property of the cells, and the combination of traumatic injury, mechanical stimuli and enzymatic digestion. The protective effects of ACSC infusion into osteoarthritis (OA) rat knees on osteochondral tissues were evaluated using micro-CT and pathological analyses. Moreover, the regulation of ACSCs on osteoarthritic osteoclasts and the underlying mechanisms in vivo and in vitro were explored by RNA-sequencing, pathological analyses and functional gain and loss experiments. The one-way ANOVA was used in multiple group data analysis.

Results
The ACSCs showed typical stem cell-like characteristics including colony formation and committed osteo-chondrogenic capacity. In addition, intra-articular injection into knee joints yielded significant improvement on the abnormal subchondral bone remodeling of osteoarthritic rats. Bioinformatic and functional analysis showed that ACSCs suppressed osteoarthritic osteoclasts formation, and inflammatory joint microenvironment augmented the inhibitory effects. Further explorations demonstrated that ACSC-derived tumor necrosis factor alpha-induced protein 3 (TNFAIP3) remarkably contributed to the inhibition on osteoarhtritic osteoclasts and the improvement of abnormal subchondral bone remodeling.

Conclusion
In summary, we have reported an easy and reproducible human ACSC isolation strategy and revealed their effects on subchondral bone remodeling in OA rats by releasing TNFAIP3 and suppressing osteoclasts in a diseased microenvironment responsive manner.

Graphical abstract
MRE11:p.K464R mutation mediates olaparib resistance by enhancing DNA damage repair in HGSOC
Authors: Zhuang Xucui , Xiao Rourou , Fu Yu , Yang Bin , Fan Junpeng , Lu Funian , Qin Tianyu , Yang Xiaohang , Hu Xingyuan , Yin Jingjing , Li Wenting , Kang Xiaoyan , Chen Gang , Hu Dianxing , Sun Chaoyang 
Published: September 2023

Abstract
Background
Although the clinical application of PARP inhibitors has brought hope to ovarian cancer, the problem of its resistance has become increasingly prominent. Therefore, clinical experts have been focused on finding specific indicators and therapeutic targets that can be used for resistance monitoring of PARP inhibitors.

Results
By cfDNA detecting during Olaparib maintenance therapy in platinum-sensitive relapsed ovarian cancer, we found the presence of MRE11:p.K464R mutation was strongly associated with acquired Olaparib resistance. Structural analysis revealed that the MRE11:p.K464R mutation is situated at a critical site where the MRE11 protein interacts with other biomolecules, leading to potential structural and functional abnormalities of MRE11 protein. Functionally, MRE11:p.K464R mutation enhanced the tolerance of Olaparib by reducing the DNA damage. Mechanistically, MRE11:p.K464R mutation improved the efficiency of DNA damage repair and induce Olaparib resistance by enhancing its binding activity with the interacting proteins (including RAD50 and RPS3). Among them, the enhanced binding of MRE11:p.K464R mutation to RAD50/RPS3 facilitated non-homologous end joining (NHEJ) repair in tumor cells, thereby expanding the scope of research into acquired resistance to PARP inhibitors.

Conclusions
Our findings provide a theoretical basis for MRE11:p.K464R mutation as a specific indicator of resistance monitoring in Olaparib treatment, and the exploration of its resistance mechanism provides a novel insights for the formulation of combination ther therapies after Olaparib resistance.
Numerical simulation of ground thermal response in Canadian seasonal frost regions to climate warming
Authors: Marrah Mohammed Yassir , Fall Mamadou , Almansour Husham 
Published: September 2023

AbstractTo ensure that public infrastructure can safely provide essential services and support economic activities in seasonal frost regions, the design of their foundation systems must be updated and/or adapted to the impacts of climate change. This objective can only be achieved, if the impact of global warming on the soil thermal behaviour in Canadian seasonal frost regions is well-known and can be predicted. In the present paper, the results of a modeling study to assess and predict the effect of global warming on the thermal regimes of grounds in three Canadian seasonal frost regions (Ottawa, Sudbury, Toronto) are presented and discussed. The results show that future climate changes will significantly affect the soil thermal regimes in seasonal frost Canadian areas. The simulation results indicated a gradual loss in the frost penetration depth due to the climate change, in the three representative sites. The frost period duration will be shorter due to climate change in the three selected regions and will completely disappear in Ottawa and Toronto. However, the impact of climate change would not appear clearly in the first 40 years “up to 2060”. The response of the ground to the effect of climate change is a function of the geotechnical characteristics of the ground and the climate conditions. The numerical tool developed and results obtained will be useful for the geotechnical design of climate-adaptive transportation structures in Canadian seasonal frost areas.
A niche-mimicking polymer hydrogel-based approach to identify molecular targets for tackling human pancreatic cancer stem cells
Authors: Murota Yoshitaka , Nagane Mariko , Wu Mei , Santra Mithun , Venkateswaran Seshasailam , Tanaka Shinji , Bradley Mark , Taga Tetsuya , Tabu Kouichi 
Published: September 2023

Abstract
Background
Pancreatic adenocarcinoma (PAAD) is one of the most fatal human cancers, but effective therapies remain to be established. Cancer stem cells (CSCs) are highly resistant to anti-cancer drugs and a deeper understanding of their microenvironmental niche has been considered important to provide understanding and solutions to cancer eradication. However, as the CSC niche is composed of a wide variety of biological and physicochemical factors, the development of multidisciplinary tools that recapitulate their complex features is indispensable. Synthetic polymers have been studied as attractive biomaterials due to their tunable biofunctionalities, while hydrogelation technique further renders upon them a diversity of physical properties, making them an attractive tool for analysis of the CSC niche.

Methods
To develop innovative materials that recapitulate the CSC niche in pancreatic cancers, we performed polymer microarray analysis to identify niche-mimicking scaffolds that preferentially supported the growth of CSCs. The niche-mimicking activity of the identified polymers was further optimized by polyethylene glycol (PEG)-based hydrogelation. To reveal the biological mechanisms behind the activity of the optimized hydrogels towards CSCs, proteins binding onto the hydrogel were analyzed by liquid chromatography with tandem mass spectrometry (LC–MS/MS), and the potential therapeutic targets were validated by looking at gene expression and patients’ outcome in the TCGA database.

Results
PA531, a heteropolymer composed of 2-methoxyethyl methacrylate (MEMA) and 2-(diethylamino)ethyl methacrylate (DEAEMA) (5.5:4.5) that specifically supports the growth and maintenance of CSCs was identified by polymer microarray screening using the human PAAD cell line KLM1. The polymer PA531 was converted into five hydrogels (PA531-HG1 to HG5) and developed to give an optimized scaffold with the highest CSC niche-mimicking activities. From this polymer that recapitulated CSC binding and control, the proteins fetuin-B and angiotensinogen were identified as candidate target molecules with clinical significance due to the correlation between gene expression levels and prognosis in PAAD patients and the proteins associated with the niche-mimicking polymer.

Conclusion
This study screened for biofunctional polymers suitable for recapitulation of the pancreatic CSC niche and one hydrogel with high niche-mimicking abilities was successfully fabricated. Two soluble factors with clinical significance were identified as potential candidates for biomarkers and therapeutic targets in pancreatic cancers. Such a biomaterial-based approach could be a new platform in drug discovery and therapy development against CSCs, via targeting of their niche.
Modeling spatial patterns of longleaf pine needle dispersal using long-term data
Authors: Blaydes Suzanne H. , Cannon Jeffery B. , Aubrey Doug P. 
Published: September 2023

AbstractBackgroundPredicting patterns of fire behavior and effects in frequent fire forests relies on an understanding of fine-scale spatial patterns of available fuels. Leaf litter is a significant canopy-derived fine fuel in fire-maintained forests. Litter dispersal is dependent on foliage production, stand structure, and wind direction, but the relative importance of these factors is unknown.ResultsUsing a 10-year litterfall dataset collected within eighteen 4-ha longleaf pine (Pinus palustrisMill.) plots varying in canopy spatial pattern, we compared four spatially explicit models of annual needle litter dispersal: a model based only on basal area, an overstory abundance index (OAI) model, both isotropic and anisotropic litter kernel models, and a null model that assumed no spatial relationship. The best model was the anisotropic model (R2 = 0.656) that incorporated tree size, location, and prevailing wind direction, followed by the isotropic model (R2 = 0.612), basal area model (R2 = 0.488), OAI model (R2 = 0.416), and the null model (R2 = 0.08).ConclusionsAs with previous studies, the predictive capability of the litter models was robust when internally validated with a subset of the original dataset (R2 = 0.196–0.549); however, the models were less robust when challenged with an independent dataset (R2 = 0.122–0.319) from novel forest stands. Our model validation underscores the need for rigorous tests with independent, external datasets to confirm the validity of litter dispersal models. These models can be used in the application of prescribed fire to estimate fuel distribution and loading, as well as aid in the fine tuning of fire behavior models to better understand fire outcomes across a range of forest canopy structures.
Probable impact of environmental radiation on thyroid swellings in areas of Eastern Hyderabad and Nalgonda
Authors: Singh Namit Kant , Hage Neemu , Prabhala Shailaja , Ramamourthy Balaji , Nagaraju Sushmitha , Kappagantu Krishna Medha 
Published: September 2023

Abstract
Introduction
Thyroid swellings are prevalent in 4 to 7% of the Indian population and are commonly present in females. Thyroid swelling has been attributed to various causes, such as ingestion of goitrogens and low iodine in the diet in benign, radiation exposure, and genetic in malignancies. Eastern Hyderabad and Nalgonda have a high fluoride content in the ground water, which can potentially disrupt thyroid functioning. Moreover, the region also harbors high uranium content and environmental radiation; hence, a retrospective analysis of the FNAC and histopathology of the thyroid swellings was done to evaluate the paradigm.

Method
A retrospective analysis of the patient’s records from February 1, 2022, to January 31, 2023, was conducted considering all the patients who presented with thyroid swelling. The variables taken into consideration were age, gender, and FNAC findings. A correlation was also made with the histopathology of the specimen of operated patients.

Result
A total of 88 medical records of the patients were evaluated, and it was determined that there was a preponderance of females with a ratio of 8.77:1. Majority of the swellings were Benign labeled as Bethesda category 2 (n = 61) followed by category 3 (n = 11). To be considered are the cases of malignancies falling into category 6, which comprise 7.95% (n = 7). The majority of the patients were seen between the ages of 31 to 40 (n = 28), followed by 41 to 50 (n = 25).
On comparing with the histopathological reports, two cases of category 5 were found to be having papillary thyroid carcinoma and another with follicular thyroid carcinoma making the number of proven malignancies to be 10.22% (n = 9). Other histopathological findings correlated with the results of FNAC.

Conclusion
Through this pilot study, we conclude that most thyroid swellings were benign, but there is a higher incidence of thyroid malignancies (10.22%), significantly higher than the country average of 2 to 4%. The higher number of malignant cases can be attributed to many factors, including high fluoride content and environmental radiation. Further studies need to be conducted, taking each element individually.
Development of a smartphone-based Turkish digits in noise test
Authors: Çetinkaya Ümit Can , Konukseven Özlem 
Published: September 2023

Abstract
Background
Pure-tone audiometry is the traditional gold standard for assessment and screening of hearing impairment, but it requires the use of calibrated devices and soundproof booth. Mobile apps can offer a good alternative to traditional methods in limited circumstances and in some emergency situations, especially when traditional methods are not available. The aim of the study was to develop an easily accessible mobile-based Turkish Digit in Noise Test screening test for the assessment of hearing.

Methods
The digits were read by a male speaker whose native language is Turkish. A mobile application was developed in accordance with the Android operating system. Twenty-five individuals with normal hearing, 25 individuals with bilateral mild sensorineural hearing loss, 25 with bilateral mild mixed hearing loss, and 25 with bilateral mild conductive hearing loss total of 100 participated in the study. All participants were between the ages of 18–60. The Turkish Digit in Noise Test mobile application was applied to the participants and the test scores were compared among the types of hearing loss.

Results
The Turkish Digit in Noise Test scores of participants with bilateral mild sensorineural hearing loss (19.4 ± 5.39) and mixed hearing loss (22.96 ± 4.52) were lower than those of participants with normal hearing (39.68 ± 6.82) (p < .01). In addition, the results of participants with bilateral mild sensorineural hearing loss (19.4 ± 5.39) and mixed hearing loss (22.96 ± 4.52) were lower than those of participants with conductive hearing loss (36.88 ± 6.31) (p < .01).

Conclusion
An easy and accessible Turkish Digit in Noise Test mobile application has been developed using which one can assess his/her hearing.
Efficiency of energy harvesting out of colored Lévy fluctuations by a harmonic oscillator with piezoelectric transducer
Authors: Giuliano M. E. , Sánchez A. D. , Combi B. , Deza R. R. 
Published: September 2023

Abstract
The efficiency of a linear kinetic energy harvester by piezoelectric transduction —when driven by colored α-stable Lévy noise— is the focus of this study. Analytical results are obtained for the harvested power (in the adiabatic approximation

and for very large load circuit's time constant τ

p
), which are accurately validated by numerical simulations. In addition, an analytical expression is obtained for the characteristic time the system takes to reach a stationary regime; this information is crucial to calculate averaged quantities through simulations, given the large dispersion between realizations due to the infinite variance of the Lévy noise.
Political Economy for Hispanic America: José Joaquín de Mora as a Bridge between Continents (1825–43)
Authors: Astigarraga Jesús , Usoz Javier , Zabalza Juan 
Published: September 2023

Abstract
In the early nineteenth century, the gradual transformation of Spain's former American territories into new independent republics required redefining their political and economic frameworks. This process demanded economic reforms, policies, and institutional changes based on the ideas of the Enlightenment and liberalism that fueled a transatlantic flow of political and economic ideas from Europe to the new republics. This article examines the crucial contribution of the Spanish economist José Joaquín de Mora to this migration of ideas. His main achievement was an institutional and economic agenda for the Republic of Chile based on classical political economy and adapted to the new republic's economic, political, and social environment. The primary economic growth process, which Mora also considered appropriate for the rest of the newly created American republics, was the result of a long and successive building process, which the research also tackles.
UNC-116 and UNC-16 function with the NEKL-3 kinase to promote axon targeting
Authors: Drozd Cody J. , Quinn Christopher C. 
Published: September 2023

ABSTRACT
KIF5C is a kinesin-1 heavy chain that has been associated with neurodevelopmental disorders. Although the roles of kinesin-1 in axon transport are well known, little is known about how it regulates axon targeting. We report that UNC-116/KIF5C functions with the NEKL-3/NEK6/7 kinase to promote axon targeting in Caenorhabditis elegans. Loss of UNC-116 causes the axon to overshoot its target and UNC-116 gain-of-function causes premature axon termination. We find that loss of the UNC-16/JIP3 kinesin-1 cargo adaptor disrupts axon termination, but loss of kinesin-1 light chain function does not affect axon termination. Genetic analysis indicates that UNC-16 functions with the NEKL-3 kinase to promote axon termination. Consistent with this observation, imaging experiments indicate that loss of UNC-16 and UNC-116 disrupt localization of NEKL-3 in the axon. Moreover, genetic interactions suggest that NEKL-3 promotes axon termination by functioning with RPM-1, a ubiquitin ligase that regulates microtubule stability in the growth cone. These observations support a model where UNC-116 functions with UNC-16 to promote localization of NEKL-3 in the axon. NEKL-3, in turn, functions with the RPM-1 ubiquitin ligase to promote axon termination.
Actin and CDC-42 contribute to nuclear migration through constricted spaces in C. elegans
Authors: Ho Jamie , Guerrero Leslie A. , Libuda Diana E. , Luxton G. W. Gant , Starr Daniel A. 
Published: September 2023

ABSTRACT
Successful nuclear migration through constricted spaces between cells or in the extracellular matrix relies on the ability of the nucleus to deform. Little is known about how this takes place in vivo. We have studied confined nuclear migration in Caenorhabditis elegans larval P cells, which is mediated by the LINC complex to pull nuclei towards the minus ends of microtubules. Null mutations of the LINC component unc-84 lead to a temperature-dependent phenotype, suggesting a parallel pathway for P-cell nuclear migration. A forward genetic screen for enhancers of unc-84 identified cgef-1 (CDC-42 guanine nucleotide exchange factor). Knockdown of CDC-42 in the absence of the LINC complex led to a P-cell nuclear migration defect. Expression of constitutively active CDC-42 partially rescued nuclear migration in cgef-1; unc-84 double mutants, suggesting that CDC-42 functions downstream of CGEF-1. The Arp2/3 complex and non-muscle myosin II (NMY-2) were also found to function parallel to the LINC pathway. In our model, CGEF-1 activates CDC-42, which induces actin polymerization through the Arp2/3 complex to deform the nucleus during nuclear migration, and NMY-2 helps to push the nucleus through confined spaces.