bluuebunny/crossref_metadata_2025_split huggingface dataset:
Irinotecan‐induced gastrointestinal damage alters the expression of peptide transporter 1 and absorption of cephalexin in rats
Authors: Imaoka Ayuko , Hattori Tomoki , Akiyoshi Takeshi , Ohtani Hisakazu 
Published: July 2023

AbstractIrinotecan causes severe gastrointestinal damage, which may affect the expression of intestinal transporters. However, neither the expression of peptide transporter 1 (Pept1) nor the pharmacokinetics of Pept1 substrate drugs has been investigated under irinotecan‐induced gastrointestinal damage. Therefore, the present study quantitatively investigated the effects of irinotecan‐induced gastrointestinal damage on the intestinal expression of Pept1 and absorption of cephalexin (CEX), a typical Pept1 substrate, in rats. Irinotecan was administered intravenously to rats for 4 days to induce gastrointestinal damage. The expression of Pept1 mRNA and the Pept1 protein in the upper, middle, and lower segments of the small intestine of irinotecan‐treated rats was assessed by quantitative real‐time polymerase chain reaction (PCR) and western blotting, respectively. The pharmacokinetic profile of CEX was examined after its oral or intravenous administration (10 mg/kg). In irinotecan‐treated rats, ∼2‐fold increases in Pept1 protein levels were observed in all three segments, whereas mRNA levels remained unchanged. The oral bioavailability of CEX significantly decreased to 76% of that in control rats. The decrease in passive diffusion caused by intestinal damage may have overcome the increase in Pept1‐mediated uptake. In conclusion, irinotecan may decrease the intestinal absorption of Pept1 substrate drugs; however, it increased the expression of intestinal Pept1.
The outcome and complications of modern staged repair surgery in newborns with classic bladder exstrophy in different genders: A retrospective study
Authors: Esmaeilizadeh Amin Haj , Ebrahimisaraj Gholamreza , Sarafi Mehdi , Rouzrokh Mohsen , Mohajerzadeh Leily , Ghoroubi Javad , Tabari Ahmad Khaleghnejad , Ebrahimian Manoochehr 
Published: July 2023

AbstractIntroductionClassic bladder exstrophy (CBE) is a rare anterior midline birth defect that remains a challenge for pediatric surgeons. Despite multiple reconstructive methods, outcomes vary widely in various reports. This study aims to compare the success rate and complications of modern staged repair of exstrophy (MSRE) in each gender and compare together.MethodsThis retrospective cross‐sectional study included cases of CBE between 2010 and 2020 that underwent MSRE. Short‐term follow‐up results, including incontinence rate, vesicoureteral reflux (VUR), urinary infections, deformed genitalia, and so on, were measured in each gender, and their differences were reported.ResultsAmong the 40 newborns with CBE who underwent MSRE, 25 (62.5%) were boys, while the others had non‐male genitalia. The rates of incontinence, VUR, dehiscence, and fistulas did not differ significantly between genders. However, chronic urinary tract infections (UTIs) were more frequent in girls, and boys were more likely to have malformed genitalia (p < .05).ConclusionOur findings indicate a similar rate of complications in each gender. However, chronic UTIs and external genitalia deformities were significantly more common in girls and boys, respectively. Further large‐sized controlled trials may be needed to corroborate these findings.
Functional analyses conducted via telehealth with caregivers as primary therapists
Authors: Farros Jesslyn N. , Legaspi David , Wallace Michele 
Published: August 2023

AbstractConducting efficient and accurate assessments prior to developing a treatment to eliminate problem behavior is necessary. When surveyed, most clinicians report functional analyses (FAs) to be the most informative in determining the function of problem behaviors when compared to descriptive and indirect methods. However, they also report not conducting FAs as a standard practice. Several barriers are reported, such as lack of time and lack of space. Most FA are designed to be conducted in‐person; however, some have utilized a telehealth service delivery model to conduct such assessments. The current study sought to extend the current telehealth FA literature and address the two most reported barriers by using a video conferencing with caregivers as the primary therapists to determine the function of problem behavior. The function of problem behavior was determined across both participants. Benefits, barriers, and future direction of utilizing such an approach are discussed.
Motivating children with feeding disorders to self‐feed: An evaluation of using differential reinforcement and manipulation of establishing operations to increase self‐feeding
Authors: Hansen Bethany A. , Phipps Laura E. , Drayton Amy K. , Demchuk Nicole C. , Knight Rachel M. , Elson Lindsey A. 
Published: August 2023

AbstractSelf‐feeding is a behavioral cusp vital to independence, growth, and development. Previous studies demonstrate that interventions like escape extinction in the form of physical guidance are effective at increasing self‐feeding in children with feeding disorders. However, these interventions may not be effective for all children. In the present study, we evaluated the effects of a treatment package that involved increasing the quality of feeder attention and access to tangibles to decrease the comparative value of escape from the self‐feeding demand for two children with feeding disorders using a nonconcurrent multiple baseline design. Despite demonstrating the skills to self‐feed preferred foods and consume target foods, neither child self‐fed target foods independently. Following differential reinforcement with the manipulation of establishing operations, both children demonstrated improvement in self‐feeding bites of target foods. In addition, caregivers were trained to implement the protocol with high procedural integrity.
Chemical Constituents and Biological Activities of Piper as Anticancer Agents: A Review
Authors: Woon Choy Ker , Ahmad Fasihuddin Badruddin , Zamakshshari Nor Hisam 
Published: July 2023

AbstractCancer has become the primary cause of death worldwide, and anticancer drugs are used to combat this disease. Synthesis of anticancer drugs has limited success due to adverse side effects has made compounds from natural products with minimal toxicity gain much popularity. Piper species are known to have a biological effect on human health. The biological activity is due to Piper species rich with active secondary metabolites that can combat most diseases, including cancer. This review will discuss the phytochemistry of Piper species and their anticancer activity. The identification and characterization of ten active metabolites isolated from Piper species were discussed in detail and their anticancer mechanism. These metabolites were mainly found could inhibit anticancer through caspase and P38/JNK pathways. The findings discussed in this review support the therapeutic potential of Piper species against cancer due to their rich source of active metabolites with demonstrated anticancer activity.
N‐terminal LysSN‐His‐tag improves the production of intracellular recombinant protein in Bacillus subtilis
Authors: Le Ngan Thi Phuong , Phan Trang Thi Phuong , Truong Tuom Thi Tinh , Schumann Wolfgang , Nguyen Hoang Duc 
Published: July 2023

AbstractChoosing fusion tags to enhance the recombinant protein levels in the cytoplasm of Bacillus subtilis has been limited. Our previous study demonstrated that His‐tag at the N‐terminus could increase the expression levels of the low‐expression gene egfp, while significantly reducing the high‐expression genes gfp+ and bgaB in the cytoplasm of B. subtilis. In this study, we aimed to prove the potential of a fusion tag, the combination of the N‐terminal domain of B. subtilis lysyl tRNA synthetase (LysSN) and His‐tag with varying numbers of histidine (6xHis, 8xHis, 10xHis) by investigating their effects on the expression levels of egfp, gfp+ and bgaB in B. subtilis. For the low‐expression gene, LysSN‐xHis‐tag could enhance the fluorescent intensity of EGFP 23.5 times higher than EGFP without a fusion tag, and 1.5 times higher than that fused with only His‐tag. For high‐expression genes, the expression level of BgaB and GFP+ was 2.9 and 12.5 times higher than that of His‐tag, respectively. The number of histidines in LysSN‐xHis‐tag did not influence the expression levels of the high‐expression genes but affected the expression levels of the low‐expression gene.
Selection of principal variables through a modified Gram–Schmidt process with and without supervision
Authors: Skogholt Joakim , Liland Kristian H. , Næs Tormod , Smilde Age K. , Indahl Ulf G. 
Published: July 2023

AbstractIn various situations requiring empirical model building from highly multivariate measurements, modelling based on partial least squares regression (PLSR) may often provide efficient low‐dimensional model solutions. In unsupervised situations, the same may be true for principal component analysis (PCA). In both cases, however, it is also of interest to identify subsets of the measured variables useful for obtaining sparser but still comparable models without significant loss of information and performance. In the present paper, we propose a voting approach for sparse overall maximisation of variance analogous to PCA and a similar alternative for deriving sparse regression models influenced closely related to the PLSR method. Both cases yield pivoting strategies for a modified Gram–Schmidt process and its corresponding (partial) QR‐factorisation of the underlying data matrix to manage the variable selection process. The proposed methods include score and loading plot possibilities that are acknowledged for providing efficient interpretations of the related PCA and PLS models in chemometric applications.
Platinum(IV)–Gold(I) Agents with Promising Anticancer Activity: Selected Studies in 2D and 3D Triple‐Negative Breast Cancer Models
Authors: López‐Hernández Javier E. , Nayeem Nazia , Cerón‐Carrasco José P. , Ahad Afruja , Hafeez Aiman , León Ignacio E. , Contel Maria 
Published: July 2023

AbstractNew heterometallic binuclear and trinuclear platinum(IV)–gold(I) compounds of the type [Pt(L)nCl2(OH){(OOC‐4‐C6H4‐PPh2)AuCl}x] (L=NH3, n=2; x=1, 2; L=diaminocyclohexane, DACH, n=1; x=2) are described. These compounds are cytotoxic and selective against a small panel of renal, bladder, ovarian, and breast cancer cell lines. We selected a trinuclear PtAu2 compound containing the PtIV core based on oxaliplatin, to further investigate its cell‐death pathway, cell and organelle uptake and anticancer effects against the triple‐negative breast cancer (TNBC) MDA‐MB‐231 cell line. This compound induces apoptosis and accumulates mainly in the nucleus and mitochondria. It also exerts remarkable antimigratory and antiangiogenic properties, and has a potent cytotoxic effect against TNBC 3D spheroids. Trinuclear compounds do not seem to display relevant interactions with calf thymus (CT) DNA and plasmid (pBR322) even in the presence of reducing agents, but inhibit pro‐angiogenic enzyme thioredoxin reductase (TrxR) in TNBC cells.
Dual Chalcogen‐Bonding Interactions for the Conformational Control of Urea**
Authors: Inoue Takumi , Ota Moe , Amijima Yui , Takahashi Haru , Hamada Shohei , Nakamura Seikou , Kobayashi Yusuke , Sasamori Takahiro , Furuta Takumi 
Published: July 2023

AbstractDual chalcogen‐bonding interactions is proposed as a novel means for the conformational control of urea derivatives. The formation of a chalcogen‐bonding interaction at both sides of the urea carbonyl group was unambiguously confirmed by X‐ray diffraction as well as computational studies including non‐covalent interaction (NCI) plot index analysis, quantum theory of atoms in molecules (QTAIM) analysis, and natural bond orbital (NBO) analysis via DFT calculations. By virtue of this dual interaction, urea derivatives that bear chalcogen atoms (X=S and Se) adopt a planar structure via the carbonyl oxygen (O) with an X⋅⋅⋅O⋅⋅⋅X arrangement on the same side of the molecule. The rigidity of the conformational lock was evaluated using the molecular arrangement in the crystal and the rotational barrier of benzochalcogenophene ring, which indicated a stronger conformational lock in benzoselenophene than in benzothiophene urea derivatives. Furthermore, the acidity of the urea derivatives increases according to the Lewis‐acidic properties of the chalcogen‐bonding interactions, whereby benzoselenophene urea is more acidic than benzothiophene urea. Tweezer‐shaped urea derivatives were prepared, and their stereostructure proved the viability of the conformational control for defining the location of the substituents on the urea framework.
Anaphylaxis management in a French pediatric emergency department: Lessons from the ANA‐PED study
Authors: Clark Evangéline , Kase Tanno Luciana , Vo Tram , Blanc Brigitte , Demoly Pascal , Caimmi Davide 
Published: August 2023

AbstractBackgroundAnaphylaxis is a serious systemic hypersensitivity reaction that requires immediate recognition and prompt administration of epinephrine/adrenaline. The present study aimed to assess the appropriateness of epinephrine/adrenaline use in children identified as allergic by physicians in the emergency department (ED) at the time of the reaction, and to identify factors that are possibly associated with epinephrine/adrenaline administration, auto‐injector prescription, and further referral to an allergist.MethodsWe performed a retrospective cross‐sectional study at the pediatric ED of the University Hospital of Montpellier, France. We included all consecutive children who attended the ED between 2016 and 2020 with an allergy‐related diagnosis at discharge.ResultsWe included 1056 allergy‐related visits, including 224 (21.2%) with a diagnosis of anaphylaxis at discharge; only 17.0% of them received an epinephrine/adrenaline injection, and 57.1% consulted an allergist after the acute episode. An auto‐injector was prescribed to 63 (28.1%) patients at discharge from the ED. Besides the severity of the clinical presentation, factors associated with a guidelines‐based management of the anaphylactic reaction and with an increased administration rate of epinephrine/adrenaline included presence of asthma symptoms and presence of extended skin reactions.ConclusionsOur study underlines persistent gaps in the management of pediatric anaphylaxis in ED, focusing on hereby identified levers. By disseminating current knowledge and guidelines on anaphylaxis and allergies, specialists could work together with emergency physicians to establish effective management algorithms and improve anaphylaxis management and care pathways for children experiencing allergic reactions, especially anaphylaxis.Trail RegistrationClinical Trials, number NCT05112367.
{111}‐ Faceted Silver Nanoplates: An Automated and Customized Design for Functionality
Authors: Pistonesi Denise B. , Belén Federico , Centurion María E. , Sica María G. , Ruso Juan M. , Messina Paula V. 
Published: August 2023

AbstractSilver nanoparticles (Ag‐NPs) exhibit the highest efficiency of localized surface plasmon resonance (LSPR) excitation that can be tuned to any wave length in the visible spectrum. Its performance depends to a large extent on its physicochemical characteristics such as size and shape; which, in turn, can be modulated by the selective growth of their crystalline facets. We used a simple direct chemical reduction method with a precise manipulation of seed‐mediated growth control through an automated single‐phase continuous flow‐batch system to induce customized geometries on Ag‐NPs. Optimization of the experimental design was carried out from a multivariate analysis, where the height/width ratio of LSPR band was used as response signal. Proposed methodology controls the critical steps in the synthesis of Ag‐NPs that modulate their morphology to attain customized surface plasmon resonance in an interval of 380 nm (spherical‐shaped nanoparticles) to 925 nm ({111}‐ faceted prism‐shaped nanoplates) absorptions, leading to a versatile platform to extend their potential applications. Although the present work focused on silver nanoparticles, we believe that this methodology can be extended to any free‐electron metals.
Concentration–QTc Relationship from a Single Ascending Dose Study of ANAVEX3‐71, a Novel Sigma‐1 Receptor and Allosteric M1 Muscarinic Receptor Agonist in Development for the Treatment of Frontotemporal Dementia, Schizophrenia, and Alzheimer's Disease
Authors: Fadiran Emmanuel O. , Hammond Edward , Tran James , Xue Hongqi , Chen Janell , Kaufmann Walter E. , Missling Christopher U. , Darpo Borje 
Published: July 2023

AbstractThis is the cardiodynamic evaluation of a single ascending dose study in healthy participants with the primary objective of assessing the effect of ANAVEX3‐71, formerly AF710B, on ECG parameters. Twelve‐lead ECGs were obtained at 3 time points within 1 hour prior to dosing to establish a baseline and then serially postdose. Concentration–QTc analysis of plasma concentrations of ANAVEX3‐71 and metabolite M8 was conducted. ANAVEX3‐71 at the studied doses did not have a clinically relevant effect on heart rate or on the PR and QRS intervals. ANAVEX3‐71 alone was retained in the primary model due to small fit differences between models which included the metabolite M8. The estimated population slope of the concentration–QTcF relationship was small and slightly negative: −0.017 ms per µg/L, with a small treatment effect‐specific intercept of −0.49 ms. An effect on the placebo‐corrected, change‐from‐baseline QTc exceeding 10 ms can be excluded within the full observed ranges of plasma concentrations of ANAVEX3‐71 and M8 up to ∼996 and ∼58 µg/L, respectively. The results from this cardiodynamic evaluation demonstrated that ANAVEX3‐71 at single ascending doses of 5‐200 mg had no clinically relevant effects on any of the studied ECG parameters.
Inclusion Complexes of a Metastable‐State Photoacid with High Acidity and Chemical Stability
Authors: Yuan Ya‐Nan , Yan Gao‐Jie , Wang Zi‐Xuan , Song Yao‐Yao , Wang Zhao‐Yang , Wang Qing‐Lun , Yang Chun , Liao Yi 
Published: July 2023

AbstractMetastable‐state merocyanine photoacids (MCHs) have been widely applied to various chemical, material and biomedical areas to drive or control chemical processes with light. In this work, stoichiometry and association constants have been determined for inclusion complexes of a photoacid MCH1 ((E)‐3‐(2‐(2‐hydroxystyryl)‐3,3‐dimethyl‐3H‐indol‐1‐ium‐1‐yl)propane‐1‐sulfonate) with β‐cyclodextrin (β‐CD), 2‐hydroxypropyl‐β‐CD (HP‐β‐CD), γ‐CD and HP‐γ‐CD by means of UV‐Vis absorption spectroscopic titrations. The inclusion complexes were studied to enhance acidity and chemical stability. Kinetic study showed that CDs stabilized the acidic metastable state and slowed its thermal relaxation. The acidity of the ground and metastable state (pKaGS and pKaMS) increased upon addition of CDs. The pKaMS of [MCH1 ⋅ (γ‐CD)2] is as low as 0.92 in comparison with 2.24 for MCH1, which is close to the lowest pKaMS values (1.20 and 1.03) reported previously, in which case the MCH1 was structurally modified with alkylammonium side chains. Addition of CDs also significantly enhanced the chemical stability of MCH1 against hydrolysis, which is one of the major concerns for the application of MCHs. In particular, the addition of HP‐β‐CD increased the half‐life of MCH1 in aqueous solution more than four‐fold. Moreover, the quantum chemical calculations confirmed the stoichiometry and analyzed the binding sites and hydrogen bonds of the inclusion complexes.
The influence of radio frequency‐based toothbrush on the accumulation of calculus and periodontal health: A randomized double‐blind controlled prospective study
Authors: Khoury Juan , Giladi Hadar Z. , Ginesin Ofir , Gabay Eran , Mayer Yaniv 
Published: July 2023

AbstractObjectivesThe use of a toothbrush with radio frequency (RF) has shown to be of benefit regarding the reduction of plaque, calculus, and dental staining and improving teeth shade compared to conventional powered and manual toothbrushes. Aim: To evaluate the efficacy of the RF toothbrush in the reduction of calculus accumulation and its effect on periodontal parameters as well as subject satisfaction as compared to an identical sham‐tooth brush.Materials and MethodsPatients who are under a strict maintenance program were included. Patients were allocated to test (RF toothbrush) or control (sham) randomly and were examined at baseline, one and three months. Clinical photos were taken and a consequential calculus assessment via ImageJ software. Clinical assessment included the following: plaque index (PI), bleeding on probing (BOP), probing pocket depth (PPD), and recession (REC). Patient satisfaction was assessed via a questionnaire.ResultsFifty‐eight patients (29 control, 29 test) were included. At baseline mean PPD, BOP, PI, REC, and calculus accumulation were similar between the groups. Mean buccal calculus was lower in the test group at one month 4.0% versus 6.7%, p < .05. Calculus accumulation within the groups was lower in the test group at 1 and 3 months when compared to baseline at the buccal aspect (2.8% vs. 8.9%, p < .05% and 3.8% vs. 8.9%, p < .05) and lingual aspect (6.7% vs. 16.5%, p < .05% and 8.9% vs. 16.5%, p < .05). No statistically significant results were found regarding periodontal parameters PPD, BOP, PI, and REC. No difference was found between groups regarding patient satisfaction.ConclusionRF seems to have an additive effect on preventing calculus accumulation on the buccal aspect of anterior mandibular teeth at 1 month. Nevertheless, at 3 months, no difference between the toothbrushes is seen regarding calculus formation and maintaining periodontal health (ClinicalTrials.gov, Identifier NCT04640857).
Recycling Graphite from Spent Lithium Batteries for Efficient Solar‐Driven Interfacial Evaporation to Obtain Clean Water
Authors: Han Sheng‐Jie , Xu Lei , Liu Pan , Wu Jia‐Li , Labiadh Lazhar , Fu Ming‐Lai , Yuan Baoling 
Published: August 2023

AbstractSolar‐driven interfacial evaporation technology is regarded as an attracting sustainable strategy for obtaining portable water from seawater and wastewater, and the recycle of waste materials to fabricate efficient photothermal materials as evaporator to efficiently utilize solar energy is very critical, but still difficult. To this purpose, graphite recovered from spent lithium‐ion batteries (SLIBs) was realized using a simple acid leaching method, and a reconstructed graphite‐based porous hydrogel (RG‐PH) was subsequently fabricated by crosslinking foaming method. The incorporation of reconstructed graphite (RG) improves the mechanical characteristics of hydrogels and the light absorption performance significantly. The evaporation rate of optimized RG‐PH can constantly reach 3.4278 kg m−2 h−1 for desalination under a one solar irradiation, and it also showed the excellent salt resistance in various salty water. Moreover, RG‐PH has a strong elimination of a variety of organic contaminants in wastewater, including the typical volatile organic compound of phenol. This research shows the potential application of flexible and durable solar evaporators made from waste materials in purifying seawater and wastewater, not only contributing to carbon neutrality by recycling graphite from SLIBs, but also ensuring the cost‐effectiveness harvest of solar energy for constantly obtaining clean water.