bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of silver, gold and platinum nanoparticles on selected nematode trophic groups
Authors: Dzięgielewska Magdalena , Skwiercz Andrzej , Wesołowska Anna , Kozacki Dawid , Przewodowski Włodzimierz , Kulpa Danuta 
Published: June 2023

Abstract
Nematodes represent different trophic groups in the soil. They therefore play an important role in the soil environment: they regulate populations of other soil organisms, mineralize nutrients into plant-available forms, provide a source of food for other soil organisms and feed on pathogenic organisms and plant pests. In contrast, plant-parasitic nematodes are important pests of crops. Nanoparticles are increasingly being used in agriculture and other production sectors. They are present in the soil, not necessarily in trace amounts, and can affect a variety of soil organisms, including nematodes. Under laboratory conditions, the effects of silver (Ag), gold (Au) and platinum (Pt) nanoparticles on the biological activity of selected nematode species from two trophic groups: entomopathogenic nematodes (Heterorhabditis bacteriophora and Steinernema feltiae) and plant parasitic nematodes (Xiphinema diversicaudatum, Ditylenchus dipsaci, Heterodera schachtii) were studied. The plant-parasitic nematodes tested responded differently to nanoparticle solutions. The greatest sensitivity to Ag nanoparticles was observed in D. dipsaci. The best effect of Au nanoparticles was observed in H. schachtii. Lead caused a positive response in D. dipsaci, in contrast to H. schachtii, compared to the control. In contrast, X. diversicaudatum and H. baltica were not affected by Pt nanoparticles. Despite similar concentrations silver nanoparticles, compared to Au and Pt nanoparticles, had the strongest toxic effects on the infective juveniles of both EPN nematode species tested. However, during the migration of a new generation of EPN infective juveniles from a dead insect, the results were inconclusive. For H. bacteriophora, the number of IJs nematodes migrating outside the cadaver after contact with AgNP and PtNP was higher compared to the control and other nanoparticles. The results encourage field research into increasing the efficacy of entomopathogenic nematode biopreparations using nanoparticles and reducing the harmfulness of plant parasitic nematodes in crops.
Effectiveness of Self-assembling Peptide (P11-4) in Conjunction With Fluoride Varnish in the Management of White Spot Lesions in Primary Teeth (Randomized Controlled Clinical Trial)
Authors: Mamdouh Claudine , Dowidar Karin ML , Soliman Reham S 
Published: June 2023

Abstract
Background
Sodium fluoride (NaF) varnish is considered the gold standard for the treatment of white spot lesions (WSLs) in primary teeth. Biomimetic regeneration using Self-Assembling Peptide P11-4 (SAP) is an alternative approach for minimal invasive treatment of initial carious lesions. This study aimed to clinically assess the effect of P11-4 in conjunction with 5% NaF varnish versus 5% NaF varnish alone, on remineralization of WSLs in primary teeth.
Methods
Twenty-four children, aged 3 to 6 years with visible active WSLs (ICDAS II score 1–3) on primary teeth were randomly allocated into 2 equal groups. The test group was treated with Curodont™ Repair [(SAP (P11-4)] and Duraflor varnish (5%NaF), while the control group was treated with Duraflor varnish alone. Before treatment, baseline assessments were done using ICDAS II scores, Nyvad criteria for lesion activity and DIAGNOdent ™ measurements. After that the materials in both groups were applied according to the manufacture’s instructions. Patients were recalled after 3 and 6 months for lesion assessment and re-application of 5%NaF. Groups were statistically compared using Mann Whitney and Chi-square tests. Changes across time were assessed within each group using Friedman test followed by post hoc test.
Results
Intergroup comparison showed a decrease in the DIAGNOdent ™ readings with no significant difference at the 3 and 6 months follow up (P = 0.297 and 0.183 respectively). The study group showed a significant difference in the DIAGNOdent ™ readings between baseline and 6 months follow up (P = 0.009) while the control group showed no significant difference (P = 0.085). At 6 months follow up, in the study group (75%) of the WSL became inactive and in the control group (50%) became inactive with no significant difference between the 2 groups (P = 0.400). Regarding the ICDAS II scores no significant change was observed throughout the different time intervals where P = 0.368 and 0.368 respectively.
Conclusions
Self-Assembling Peptide in conjunction with 5% NaF varnish showed superior remineralization of WSLs compared to 5% NaF varnish only after 6 months follow up.
Trial registration:
the clinical trial was registered in ClinicalTrials.gov under the number NCT05721586 (10/02/2023)
Micropropagation of Ilex cassine ‘angustifolia’
Authors: Gui Qing , Zou Yiping , Zhang Donglin , Hao Mingzhuo , Yang Yujie 
Published: June 2023

Abstract
For mass propagation of Ilex cassine ‘angustifolia’, tissue culture was used to shorten its propagation cycle. We sterilized its nodal segments as explants and then cultured them on MS basic medium supplemented with different concentrations of 6-BA and NAA, and placed them in the culture room for the induction and proliferation of adventitious shoots. The highest induction rate of MS medium with 6-BA 1.0 mg/L and NAA 1.0 mg/L was used for the induction of adventitious buds in Ilex cassine ‘angustifolia’ nodal segments, reaching 80%. Buds sprouted on the 10th day, while the average value of bud length reached 1.62 cm at 30 days. The adventitious buds grew fast and robust. The best proliferation coefficient was 5.7 when explants were cultured on MS basic medium containing 6-BA mg/Land NAA 0.5 mg/L. For adventitious root induction, the adventitious shoots were transferred to MS basic medium with different concentrations of IBA and NAA. The results showed that the highest rooting rate of 98.7% was achieved when explants were cultured on MS basic medium supplemented with NAA 0.5 mg/L and IBA 1.0 mg/L, and the adventitious roots induced were thick and robust. Rooted sterile seedlings had been planted in the substrate and transferred to a greenhouse with sprinkler irrigation for further cultivation. The survival rate was about 90%.
Sub-coronary aortic root replacement post-aortic valve dehiscence: A Case Report
Authors: Divya Aabha , Mangel Tobin , Seetharaman Rakesh , Taghavi John Fouad 
Published: June 2023

Abstract
This is a case report of a 78-year-old male who underwent a sub-coronary aortic valve and root replacement due to valve dehiscence and aortic root pseudoaneurysm. The patient had complex anomalous coronary anatomy and had undergone a previous tissue aortic valve replacement in 2013. The patient made an uneventful recovery and was discharged from the hospital five days later. The authors suggest that the sub-coronary root replacement technique should be considered in elderly patients and patients with complex coronary anatomy.
Conductive Glassy Non-conjugated Open-shell Radical Polymer with Organosulfur Backbone for Macroscopic Conductivity
Authors: Thi Quyen Vu , Nguyen Quynh H. , Jeon Seung-Yeol , Boudouris Bryan , Joo Yongho 
Published: June 2023

Abstract
Non-conjugated organic radical with open-shell radical active group exhibits unique functionality due to their radical pendant site. Compared to the previously studied doped conjugated polymer, radical polymers reveal superior processability, stability, and optical properties. Despite the success of organic radical polymer conductors based on the TEMPO radicals, it still requires the potential designing substitutions to meet the fundamental limits of charge transport in the radical polymer. To do so, we demonstrate that the amorphous, non-conjugated radical polymer with backbone-pendant group interaction and low-glass transition temperature enables the macromolecules to have rapid charge transport in the solid state, resulting in conductivity higher than 32 S m-1. This charge transport is due to the formation of the local ordered regime with energetically favored orientation caused by the strong coupling between the backbone and pedant group, which can significantly modulate the polymer packing with active electronic communications. The non-conjugate nature of radical polymer maintains the optical transparency up to 98% at 1.5 μm thick film. Thus, this effort will be a dramatically advanced model in the organic radical community for the creation of next-generation polymer conductors.
Screening for axonal retraction and cytotoxicity using dorsal root ganglia explants to treat pain caused by aberrant nerve sprouting
Authors: Lee Fei San , Nguyen Uyen N. , Munns Eliza J. , Wachs Rebecca A. 
Published: June 2023

Abstract
Low back pain, knee osteoarthritis and cancer patients suffer from chronic pain. Aberrant nerve growth into intervertebral disc, knee, and tumors, are common pathologies that lead to these chronic pain conditions. Retraction of nerve fibers via capsaicin (Caps) or resiniferatoxin denervation have been used to treat painful neuropathies and knee osteoarthritis but with short-term efficacy and discomfort. Herein, we propose to repurpose pyridoxine (Pyr), vincristine sulfate (Vcr) and ionomycin (Imy) as potential axonal retraction compounds for denervation. Previous literature suggests Pyr, Vcr, and Imy each have undesired axonal degeneration as an off-target effect. Thus, we performed axonal retraction phenotype screening using adult rat dorsal root ganglia explants in vitro and assessed neurotoxicity. Imy did not induce axonal retraction, while Pyr and Vcr produced robust axonal retraction within three days. All DRGs treated with Caps, Pyr, Vcr, Imy or solvent had minimal neurotoxicity. Further, neither Pyr nor Vcr triggered nucleus pulposus cell death or affected cellular metabolic activity after three days of incubation. Overall, our findings suggest Pyr and Vcr are non-cytotoxic to dorsal root ganglia and nucleus pulposus cells, and there is potential for repurposing as axonal retraction compounds for local denervation.
Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells
Authors: Cherianidou Anna , Kappenberg Franziska , Seidel Florian , Papazoglou Panagiota , Hescheler Jürgen , Leist Marcel , Hengstler Jan , Rahnenführer Jörg , Peng Luying , Acharya Aviseka , Sriniva Sureshkumar Perumal , Sachinidis Agapios 
Published: June 2023

Abstract
Animal studies for embryotoxicity evaluation of potential therapeutics and environmental factors are complex, costly, and time-consuming. Often, studies are not of human relevance because of species differences. In the present study, we recapitulated the process of cardiomyogenesis in human induced pluripotent stem cells (hiPSCs) by modulation of the Wnt signaling pathway to identify a key cardiomyogenesis gene signature that can be applied to identify compounds and/or stress factors compromising the cardiomyogenesis process. Among the 23 tested teratogens and 16 non-teratogens, we identified three retinoids including 13-cis-retinoic acid that completely block the process of cardiomyogenesis in hiPSCs. Moreover, we have identified an early gene signature consisting of 31 genes and associated biological processes that are severely affected by the retinoids. To predict the inhibitory potential of teratogens and non-teratogens in the process of cardiomyogenesis we established the “Developmental Cardiotoxicity Index” (CDI31g) that accurately differentiates teratogens and non-teratogens to do or do not affect the differentiation of hiPSCs to functional cardiomyocytes.
Effect of high doses of selenate application on melatonin synthesis, plant growth, sugar content, and reutilization efficiency of nutrition in sweet cherry
Authors: Sun Xieping , Yang Sha , Ling Min , Xiang Yuhui , Li Hongqun 
Published: June 2023

Abstract
Purpose: Root application of low-dose selenate does not significantly influence the melatonin content in potted sweet cherry leaves. We aimed to investigate the effects of high doses of selenate on sweet cherry plants to clarify the physiological and biochemical aspects of adaptive stress.
Methods: Different doses of 5-methoxytryptophan was foliar applied on the long branch leaves of the ‘Russia No. 8’, and the potted and field grown sweet cherry plants were treated with 0, 48, 96, 144, and 196 mg/plant. The substance content and enzyme activity involved in tryptophan metabolism were detected and the growth parameters were detected. The C, N, Se, sugar, and soluble protein contentsin mature, old, and fallen leaves in August, and in yong leaves in March of the following year.
Results: 15 mg/L of 5-methoxytryptophan significantly increased leaf melatonin content. Selenate significantly increased the melatonin content only at a dose of 96 mg/plant. Doses lower than 96 mg/plant increased plant growth parameters, whereas higher doses decreased plant growth parameter. Different leaf ages of sorbitol, amylaceum, fructose, saccharose, rhamnose, and maltose content was influenced by different Se doses treatments. With an increase in the Se dose, the leaf nitrogen content increased, and the carbon content was influenced slightly in field-cultivated trees. Conclusion: Leaf Se content was mainly distributed in mature leaves in response to low doses of Se and in old leaves in response to high doses of Se; The neat year, different Se dose also influence the leaf nutrition and sugar contents.
TGFBR1*6A enhances breast cancer development through downregulation of p27
Authors: Agyemang Kojo , Johansen Allan , Barker Grayson , Pennison Michael , Jimenez Hugo , Sheffield Kimberly , Wang Minghui , D'Agostino Ralph , Watabe Kounosuke , Cristofano Antonio di , Pasche Boris 
Published: June 2023

Abstract
TGFBR1*6A (rs1466445) is a common low penetrance tumor susceptibility allele with a minor allelic frequency of 0.051. Several meta-analyses show that TGFBR1*6A is associated with breast cancer risk with an OR ranging from 1.15 to 1.30. We developed a novel knockin mouse model of TGFBR1*6A to study mammary cancer development and progression in MMTVneu mice. Here we show that TGFBR1*6A accelerates tumor onset, increases the percentage of mice with tumors, and the percentage of mice with multiple tumors. Age at tumor onset, tumor formation, and tumor multiplicity depend on TGFBR1*6A allelic dosage. Functional characterization shows that TGFBR1*6A confers higher tumor growth and clonogenicity and enhances G0/G1 cell cycle progression through downregulation of p27. These findings establish TGFBR1*6A as a potent modifier of breast cancer development and progression, which may affect more than 10% of patients with breast cancer.
The impact of time interval on prognosis in patients with non-small cell lung cancer brain metastases after metastases surgery: a retrospective study
Authors: Yang Zhi , Chen Haibin , Jin Tao , Sun Liang , Li Liwen , Zhang Shuyuan , Wu Bin , Jin Kai , Zou Yangfan , Xia Liang , Sun Caixing 
Published: June 2023

Abstract
Purpose
Lung cancer is a leading cause of cancer-related deaths worldwide and frequently gives rise to brain metastases (BM), which significantly worsen patient prognosis. This study aimed to identify risk factors associated with overall survival (OS) following non-small cell lung cancer (NSCLC) brain metastases (BM) surgery and to assess the impact of treatment timing on OS.
Methods
We analyzed data from 74 patients (2011–2021) who underwent BM surgery at our institution. The relationship between various clinical, radiological, and histopathological factors, as well as time intervals (TI) and OS, was examined.
Results
The median TI was 19 months (range: 9–36 months). Shorter TI < 23 months (aOR 2.87, 95% CI 1.03–8.02, p = 0.045), age ≥ 60 years at BM diagnosis (aOR 2.51, 95%CI 1.07–5.92, p = 0.035), initial M stage of lung cancer as M1 (aOR 2.80, 95%CI 1.04–7.59, p = 0.043), postoperative adjuvant radiotherapy for BM (aOR 0.50, 95%CI 0.25–0.99, p = 0.047), and molecular targeted therapy after BM (aOR 0.15, 95%CI 0.06–0.35, p = 0.015) were independently factors associated with OS after brain metastases surgery. Late-onset BM (TI ≥ 36 months) was observed in 15 cases (20.3%), and it was independently associated with an age at lung cancer diagnosis of ≥ 60 years [adjusted odds ratio (aOR 7.24, 95%CI 1.59–32.95, p = 0.011] ,targeted therapy (aOR 2.27, 95%CI 1.70–3.03, p༜0.001)and absence of adjuvant chemotherapy (aOR 6.46, 95%CI 1.52–27.43, p = 0.011).
Conclusions
Multiple factors impact the TI between NSCLC and BM. The occurrence of brain metastases (BM) within 23 months after a diagnosis of non-small cell lung cancer (NSCLC) was independently associated with a worse prognosis following BM surgery. In NSCLC patients aged 60 years or older who did not receive adjuvant chemotherapy, there was an increased likelihood of developing brain metastases (BM) after a long progression-free survival period. Additionally, postoperative patients with brain metastases from non-small cell lung cancer should undergo genetic testing to determine the need for targeted molecular therapy. These findings highlight the importance of careful follow-up and consideration of targeted therapy to potentially prolong the survival of these patients.
Peer support interventions in maternal and child healthcare delivery in sub-Saharan Africa: Protocol for a realist review
Authors: Dugle Gordon , Antwi John , Quentin Wilm 
Published: June 2023

Abstract
Background
Peer support has been proposed as a promising policy intervention for addressing adverse maternal and child healthcare (MCH) outcomes in sub-Saharan Africa (SSA). Existing reviews on peer support largely draw on evidence from high-income countries or focus on single services like breastfeeding, nutrition or postnatal care. In contrast, this review aims at providing a comprehensive overview of the empirical literature on peer support interventions across various MCH services in sub-Saharan Africa. Specifically, we aim to understand how, why, for whom, and in what circumstances different forms of MCH peer support interventions contribute to improving healthcare outcomes in sub-Saharan Africa.
Methods
Our realist review methodology involves various iterative processes of search, appraisal, selection and synthesis of the literature. Four databases–Cochrane Library, PubMed, CINAHL, and EMBASE–were repeatedly searched between March and June 2021. From a large volume of records retrieved from the database and citation search, 61 papers have been selected for review. We will conduct a second search of the same database covering June 2021 to the present before the final extraction and synthesis. The final list of selected papers will be imported into NVivo 12 software and organised, extracted, analysed and synthesised iteratively to examine and illustrate the causal links between contexts, mechanisms and outcomes of MCH peer support interventions in SSA.
Discussion
The review will develop an explicit theory of peer support intervention in healthcare delivery and provide insights for developing evidence-informed policy on the intervention. Drawing lessons from the different national contexts and diverse areas of MCH in SSA, the review will provide an analytically generalizable programme theory that can guide intervention design and implementation. While focusing on MCH peer support interventions in SSA, the review contributes to evolving conversations on the use of theory for health policy planning and complex intervention design and implementation globally.
Systematic review registration: Submitted in PROSPERO (18/05/2023)
Evaluation of Experiences of the Patients Discharged from the COVID-19 Intensive Care Unit: A Qualitative Research
Authors: Torun Serap , Bulmuş Esra , Bilgin Osman 
Published: June 2023

Abstract
Background
Making arrangements by learning how intensive care patients feel due to a disease called as fatal worldwide can make it easier for patients to cope with the disease. For this reason, it is important for healthcare professionals to understand the patients who have been infected and discharged during the COVID-19 pandemic. The experiences of the patients may affect the perspective of the disease and cause different changes in the perception of it. This study, which was conducted based on this idea, aimed to examine the intensive care experiences of patients discharged from the COVID-19 intensive care unit.
Method
This study used a phenomenological qualitative approach. A semi-structured interview form was used to interview 23 patients discharged from the COVID-19 intensive care unit. The findings were reported on the basis of consolidated criteria for reporting qualitative research.
Findings:
In line with the data obtained from the interviews, five main themes and eight subthemes were created. The main themes were classified as emotional expressions (positive/negative) related to intensive care experience, coping methods, care experiences (respiration, nutrition, excretion and privacy, sleep, communication) and analogies for COVID-19 and nurses.
Conclusion
In this study, the participants experienced negative emotions such as fear of death/anxiety, sadness, loneliness, and helplessness during their intensive care experiences. Most of them stated that they tried to cope with prayer and communication. The participants compared COVID-19with deadly and respiratory-inhibiting tools and diseases. They expressed difficulties in breathing, nutrition, excretion and privacy, sleep and communication related to the care provided. In this process, they made positive analogies for the nurses who spent the most time with them, such as angels and family members.
DNA methylation difference in the liver of a fish with female-biased sexual-size dimorphism, Ancherythroculter nigrocauda
Authors: SUN Yanhong , HU Zhen , LI Qing , CHEN Jian , LI Pei , WEI Huijie , SONG Xinhua , FU Beide , WANG Guiying 
Published: June 2023

Abstract
Methylation is one of the most important epigenetic modifications in fishes. However, difference of methylation profiles about fish with sexual-size dimorphism (SSD) is really rear. In this study, we used Methyl-RAD to study the methylation profile in the liver of fish with female-biased SSD, Ancherythroculter nigrocauda. In total, we found 6,83 million methylated cytosine in the whole genome and 4.12 million of them were present in at least 5 samples. We also found the average methylation level for female liver was significantly higher than that of male samples (p = 0.03175). And 1,137 differentially methylated genes (DMGs) were identified, many of which were growth-rate related. These DMGs were enriched in several GO catalogs, such as fatty acid synthase activity (GO:0004312), insulin-like growth factor I binding (GO:0031994), and swimming behavior (GO:0036269). The results in this study provided more insights about the epigenetic role of DNA methylation on growth-related genes’ regulation.