bluuebunny/crossref_metadata_2025_split huggingface dataset:
The nocturnal swarming activity of the malaria mosquito Anopheles coluzzii: it’s not just the males.
Authors: Vielma Sofia , Minuz Roxana , Trusso Alessandro , Sawadogo Simon , Cribellier Antoine , Diabaté Abdoulaye , Muijres Florian , Simoni Alekos , Müller Ruth 
Published: July 2023

Abstract
Anopheles gambiae sensu lato is the primary malaria vector, endemic to the African continent and has been found to mate in large swarms. To date, the spatial-temporal characterization of mosquito swarms has been restricted to male-focused interactions and relying on visual observations of field events during sunset. We recorded large-cage laboratory simulated swarm events of Anopheles coluzzii with Photonic Fence Monitoring Device (Photonic Sentry, USA) to characterize various spatial-temporal aspects of male, female, and mixed-sex swarms prior and posterior sunset. Results suggests that male and female mosquitoes form swarms in a spatially similar manner with no visible structural and physical variations within the tested groups. The study of temporal dynamics of swarms reveals a unique composition of swarm hotspot formation for up to one hour in total during night, which is contrary to literature. Our results challenge the paradigm that female mosquitoes are acting as reactive individuals to male activity only. We demonstrate that females can form stable and comparable swarms to those of only males or mixed sex, at least under laboratory conditions. This discovery merits further research regarding the purpose of female swarming activity and the missing field observations of females during sunset.
My Diet Study: A Protocol for An Observational, Longitudinal, Cross-Sectional Psycho- Biological Study of Dieting in Young People
Authors: Okada Mirei , Pehlivan Melissa , Miskovic-Wheatley Jane , Barakat Sarah , Griffiths Kristi , Touyz Stephen , Simpson Stephen J. , Maguire Sarah , Holmes Andrew 
Published: July 2023

Abstract
Background
Self-directed dieting (i.e., unsupervised) is very common among adolescents and young adults but has had almost no direct research. This paper describes the protocol for the My Diet Study, a two-arm observational investigation of the natural progression of dieting among young people over a period of 6-months. The study aims to examine the links between self-directed dieting, general physiological and psychological metrics of wellbeing (e.g., depressive symptoms) and biomarkers of gut-brain axis functions (e.g., microbiome and hormones) that are predicted to influence diet adherence through appetite, mood and metabolism regulation.
Methods
Young people aged 16-25, intending to start a diet will be invited to participate in this observational study. For Part 1 (psychological arm), participants will be asked to complete a set of questionnaires and diaries at the beginning of every month for 6 months, to assess overall mental (e.g., psychological distress, disordered eating) and physical (e.g., weight) health, perceived diet success, food intake and gastrointestinal movements. For Part 2 (biological arm), a subsample of 50 participants will be asked to provide faeces, blood and saliva for bio-sampling each month for the first 3-months of their participation in Part 1.
Discussion
The My Diet Study will be the first longitudinal, observational study of dieting in young people combining in-depth psychological and biological data. It is anticipated that the findings will yield psychological & biological information about the impacts and effectiveness of self-directed dieting in young people, inform a framework for advice on safety in dieting among young people and help to establish the potential for biomarkers for risk management and improvement of diet-based lifestyle interventions.
Resumption of intravenous anti-cancer therapy in breast cancer: a real-world experience during the SARS-CoV-2 pandemic
Authors: Yang Fang , Li Cong , Qiu Yuling , Chen Xinjie , Zheng Kelin , Kang Donglin , Zhang Yin , Liu Baorui , Yao Yongzhong , Xie Li 
Published: July 2023

Abstract
Purpose
The coronavirus disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, has delayed medical consultations, especially for patients receiving intravenous anti-cancer therapy. We aimed to investigate alterations in immune function among breast cancer patients who experienced delayed intravenous therapy due to SARS-CoV-2 infection.
Methods
We performed an observational investigation of breast cancer patients in Nanjing Drum Tower Hospital from December 27, 2022 to January 20, 2023. The patients recovered from SARS-CoV-2 infection were eligible for enrollment. Peripheral blood samples were taken prior to the restart of intravenous anti-cancer therapy to examine hematologic parameters.
Results
A total of 131 patients were included in the final analysis. Cough (74.0%), fever (62.6%), and expectoration (46.6%) were identified as the most presenting symptoms of SARS-CoV-2 infection in breast cancer. The average nucleic acid conversion time and delayed treatment time was 13.4 days and 13.9 days, respectively. The patients > 60 years old experienced prolonged nucleic acid conversion time (P = 0.017) and delayed treatment time (P = 0.028) compared to those < = 60 years old. Dysregulated lymphocyte subsets and cytokines were found post-SARS-CoV-2 infection. Treatment-related adverse events of grade 3 or 4 occurred in 7.6% after resuming intravenous anti-cancer therapy.
Conclusions
Our findings reveal that the SARS-CoV-2 infection led to imbalanced immune responses and postponed intravenous anti-cancer therapy in breast cancer. The safety report encourages the timely resumption of intravenous anti-cancer therapy after adequately weighing the risks and benefits.
Are TERT promoter mutations a poor prognostic factor in anaplastic thyroid carcinoma?
Authors: Ryu Hyun Jin , Oh Young Lyun , Heo Jung , Park Hyunju , Kim Tae Hyuk , Kim Sun Wook , Chung Jae Hoon 
Published: July 2023

Abstract
Purpose
Telomerase reverse transcriptase (TERT) promoter mutations are a poor prognostic factor in differentiated thyroid carcinoma (DTC). However, their prognostic value in anaplastic thyroid carcinoma (ATC) is unclear. Therefore, we investigated whether TERT promoter mutations also act as an independent poor prognostic factor in ATC.
Methods
We reviewed the medical records of 28 patients with ATC who underwent the TERT promoter mutations test at Samsung Medical Center between November 1995 and May 2020. The aggressive treatment group was defined as patients who underwent surgery, external radiotherapy, and systemic therapy.
Results
Among 28 patients, TERT promoter mutations were found in 10 patients (35.7%). There were no differences in the clinicopathological characteristics between the TERT-mutant and wild-type groups except tumor size and treatment modality. Median tumor size in the TERT-mutant group was 5.9 cm (3.7–11.0), which was significantly larger than that in the wild-type group (4.2 cm, 0.8–6.8, P = 0.006). Aggressive treatment was performed more frequently in the TERT-mutant group (60.0% vs. 22.2%, P = 0.046). The median overall survival (OS) was 6.9 months (0.4–39.5). The OS of the TERT-mutant group was longer that of than the wild-type group, but the difference was not significant (9.1 months [0.4–39.5] vs. 6.1 months [0.4–39.0], P = 0.432). In multiple regression analysis, old age (≥ 68.5 years), lymph node metastasis, and distant metastasis were significant prognostic factors, but TERT promoter mutations were not.
Conclusion
Unlike DTC, TERT promoter mutations were not an independent poor prognostic factor in ATC.
Propyl gallate induces cell death in human pulmonary fibroblast through increasing reactive oxygen species levels and depleting glutathione
Authors: Park woo hyun 
Published: July 2023

Abstract

Background Propyl gallate (PG) has demonstrated anti-growth effects on various cell types. This study aimed to investigate the impact of PG on reactive oxygen species (ROS) and glutathione (GSH) levels in primary human pulmonary fibroblast (HPF) cells. Additionally, the study explored the effects of N-acetyl cysteine (NAC), L-buthionine sulfoximine (BSO), and small interfering RNA (siRNAs) targeting antioxidant genes on ROS, GSH levels, and cell death in PG-treated HPF cells. Methods and Results Intracellular ROS and GSH levels were measured using different fluorescent probe dyes (H2DCFDA, DHE, MitoSOX™Red and CMFDA) through flow cytometer analysis. PG treatment at concentrations ranging from 100 to 800 µM resulted in increased levels of total ROS and O2•−at early time points (30–180 min and 24 h). Higher concentrations of PG (800–1600 µM) led to an increased number of cells depleted of GSH at 24 h and reduced GSH levels at 30–180 min. PG treatment downregulated the activity of superoxide dismutase (SOD) and upregulated the activity of catalase in HPF cells. Treatment with 800 µM PG increased the number of apoptotic cells and cells with loss of mitochondrial membrane potential (MMP; ∆Ψm) as assessed by Annexin V-FITC/PI and rhodamine 123 staining, respectively. NAC treatment mitigated PG-induced cell death and loss of MMP in HPF cells, accompanied by a decrease in GSH depletion. Conversely, BSO exacerbated cell death and MMP loss induced by PG, independent of ROS and GSH depletion levels. Following transfection with siRNA, the HPF cells were exposed to PG treatment. The siRNAs against SOD1, SOD2, and catalase successfully attenuated cell death in PG-treated HPF cells. In contrast, siRNA against GSH peroxidase enhanced cell death. Conclusions PG induced cell death in HPF cells by increasing ROS levels and depleting GSH. NAC was found to decrease HPF cell death induced by PG, while BSO enhanced cell death. The findings suggest that modulating the antioxidant defense system can influence the cytotoxic effects of PG in HPF cells.
Genome-wide genetic mutations accumulated in genome-edited pigs and their filial generation for xenotransplantation
Authors: Du Xiaoyan , Huo Xueyun , Sun Xianhui , Xing Xiangyang , Lu Jing , Zhang Jingjing , Jiang Yanyan , Zhu Xiao , Li Changlong , Lv Jianyi , Guo Meng , Cao Lixue , Liu Xin , Chen Zhenwen , Pan Dengke , He Shunmin , Zhang Chen 
Published: July 2023

Abstract
Although xenotransplantation has revolutionarily developed based on the usage of genome-edited pigs, it is still unknown whether these pigs and their offspring remains genomic stable. Here, we show that GGTA1-knockout (GTKO) pigs accumulated an average of 1205 genome-wide genetic mutations, and their filial 1 offspring contained an average of 18 de novo mutations compared with wild control and their parents, respectively. The majority of mutations were annotated in intergenic regions without altering protein functions and predicted off-target mutations. RNA-sequencing analysis and phenotype observation indicated that accumulated mutations may have a limited influence on GTKO pigs and most genetic mutations in GTKO pigs can be explained by electrotransfection of plasmids into cells. This is the first report that the genetic mutations of genome-edited pigs are inherited stably by their filial generation and provide a reference for safe application and the standard approach to breeding genome-edited pigs for xenotransplantation.
High-throughput sequencing unveils HBV DNA integration and its oncogenic role in gastric cancer
Authors: He Yifu , Li Mengge , Wu Shushen , Niu Jiayu , Luo Huiqin , Chen Wenju , Cao Lulu , Yan Ying 
Published: July 2023

Abstract
Growing evidence showed an association between hepatitis B virus (HBV) infection and gastric cancer (GC). HBV DNA integration is one of the key mechanisms contributing to hepatocellular carcinoma (HCC) development. However, the status of HBV integration in GC has not been studied yet. In this study, HBV DNA was detected in 7/10 GC and 8/10 para-tumor tissues. By high-throughput viral integration detection and long-read sequencing, a total of 176 and 260 HBV integration breakpoints were identified from GC and para-tumor tissues, respectively. In the HBV genome, the breakpoints were more frequently occurred at X gene and C gene. In the host genome, these breakpoints distribution was correlated with CpG islands. Seven protein-coding genes and one non-coding RNA genes were inserted by HBV DNA for more than once in different samples. Combined with the bioinformatics analysis and functional experiments, we highlight SPRY3 and CHD6, as potential driver genes for GC. Besides, we also revealed the spatial relationship of HBV integration and its various structural variations. Taken together, our results first indicated that HBV DNA can integrate in GC. These findings provide insight into the HBV integration and its oncogenic progression in GC.
Developing Global Open Access COVID-19 Education for Frontline Healthcare Workers
Authors: Christianson Jacqueline , Frank Erica , Keating Stacen , Chickering Miriam 
Published: July 2023

Abstract
Background: Early in the Covid-19 pandemic, we identified a heightened need for a reliable, high-quality, accessible, and evidence-based educational resource for frontline healthcare workers. Open access virtual education can reduce disparities in access to education by minimizing cost barriers and providing equitable access to educational content. Our team of global healthcare educators responded by creating an open access competency-based online course to address access disparities around Covid-19 information. The course was aimed toward frontline healthcare workers globally and included design elements such as a built-in language translation tool and non-linear course design to facilitate access and address the individual’s educational needs.
Methods: Pre- and post-course surveys were collected to evaluate how the course addressed learner needs. Data were collected between the course launch in April 2020 through December 2020.
Results: An initial population of students (N=149) ranging from high school through doctoral education, living in 23 different countries, speaking 22 different native languages took the course and participated in the pre- and/or post-course surveys. Overall, participants rated the course highly.
Conclusion: Open access educational models can facilitate equitable access to education for a global audience.
Population-based Evidence for Using Serum Neurofilaments as Individual Diagnostic and Prognostic Biomarkers in Amyotrophic Lateral Sclerosis
Authors: Witzel Simon , Huss André , Nagel Gabriele , Rosenbohm Angela , Rothenbacher Dietrich , Peter Raphael , Baezner Hansjörg , Boertlein Axel , Dempewolf Silke , Schabet Martin , Hecht Martin , Kohler Andreas , Opherk Christian , Naegele Andrea , Sommer Norbert , Lindner Alfred , Alexudis Christoforos , Bachhuber Franziska , Halbgebauer Steffen , Brenner David , Ruf Wolfgang , Weiland Ulrike , Mayer Benjamin , Schuster Joachim , Dorst Johannes , Tumani Hayrettin , Ludolph Albert 
Published: July 2023

Abstract
Neurofilaments are biomarkers for neuroaxonal damage and are being evaluated in Amyotrophic Lateral Sclerosis (ALS) to support diagnosis, estimate prognosis, and monitor treatment effects. This study presents data on serum neurofilament levels in ALS on a population level to improve their application as individual diagnostic and prognostic biomarkers. We measured neurofilament light (NfL) and phosphorylated heavy chain (pNfH) levels in a large cohort of patients with ALS and controls from the epidemiological ALS registry Swabia. We observed that serum NfL was superior to pNfH. Using NfL Z-Scores instead of raw data and incorporating ALS-independent and ALS-specific influencing factors improved the diagnostic and prognostic ability. The high predictive value and individual longitudinal validity of serum NfL indicate its utility as an individual surrogate marker for survival and disease progression. Combiningepidemiological data and biomarker research in population-based Z-Scores might be a conceptual model of interest for other neurodegenerative diseases.
The solution comparison of fractional Heat transfer and porous media equations using analytical techniques
Authors: Khan Shahbaz , Sohail Muhammad , Khan Hassan , Tchier Fairouz , Omid Mohammad yar , Nadeem Muhammad 
Published: July 2023

Abstract
In this paper, the mathematical model related to the physical problem of electrostatic or gravitational area in the fractal domain is the Poisson equation, which analyses the potential area arising from a given charge with the possibility of the area identified being considered in fractional form. The Laplace residual power series method and the Laplace Adomian decomposition technique are used to compare the solutions of the fractional local Poisson equation. The phenomenon of electromagnetic mechanisms is typically modelled using elliptic partial differential equations. Furthermore, the Poisson equation is examined using the concept of fractional local derivative. For this reason, a few examples are presented to understand the fractional local Poisson equation in its more accurate form. The results show the simple and sophisticated procedures of the two proposed analytical approaches where partial differential equations are considered with fractional derivatives or local derivatives. The outcomes of the described methods demonstrate that they have an accurate algorithm to construct with exceptionally precise cost calculation capabilities. The present research is more related to the fractional local Poisson equation and also to the fractional local derivative of the Poisson equation, which produces pleasing results.
Proteomic Biomarkers for Survival in Systemic Sclerosis-associated Pulmonary Hypertension
Authors: MISMETTI Valentine , DELAVENNE Xavier , MONTANI David , BEZZEGHOUD Souad , DELEZAY Olivier , HODIN Sophie , Launay David , MARCHAND-ADAM Sylvain , NUNES Hilario , Ollier Edouard , REYNAUD-GAUBERT Martine , Pastre Jean , TRACLET Julie , QUETANT Sébastien , ZEGHMAR Sabrina , BERTOLETTI Laurent , COTTIN Vincent 
Published: July 2023

Abstract

BACKGROUND:interstitial lung disease (ILD) and pulmonary hypertension (PH) represent the major causes of mortality in systemic sclerosis (SSc). Patients with systemic sclerosis and combined PH and ILD (SSc–PH-ILD) generally have a poor prognosis. Predictors of survival and of potential benefit of treatment are lacking in patients with SSc-PH-ILD.OBJECTIVE:To identify specific plasma protein expression patterns associated with survival in patients with SSc-PH-ILD.MATERIALS AND METHODS:Post-hoc analysis of a prospective multicenter French study in patients with PH-ILD. An untargeted proteomic analysis using mass spectrometry was performed to identify plasma protein changes associated with long-term overall survival in patients with SSc-PH-ILD.RESULTS:Thirty two patients were included in the analysis, of whom 13 died during follow-up (median survival: 76.5 months). At baseline, survivors had less severe hemodynamic impairment (pulmonary vascular resistance of 4.4 Wood Units [IQR 3 - 5.2] vs. 6.2 Wood Units [IQR 4.2 - 10.7]) and higher carbon monoxide diffusing capacity (median 39% [IQR 35% - 44%] vs. 25% [IQR 22% - 30.5%]), than the 13 patients who died. Seven proteins, associated with haemostasis and fibrosis, were differentially expressed according to patients’ survival. In the survivor group, two proteins were increased (ADAMTS13, SERPIND1) and five were decreased (PTGDS, OLFM1, C7, IGFBP7, FBN1) compared to the non-survivor groups.CONCLUSION:The prognosis of SSc-PH-ILD patients is poor. This proteomic approach found 7 plasma proteins (involved in haemostasis and fibrosis pathways) associated with survival. These potential biomarkers may be good candidates to prognostic enrichment.
Deciphering Tumor Metastasis and Immune Inhibitory Signature of Clear Cell Renal Cell Carcinoma by Single-Cell Transcriptome Analysis
Authors: Yin Xinbao , Wang Ying , Wang Ke , Zhu Guanqun , Zhang Zongliang , Zhao Kai , Zhang Shaojun , Shi Benkang 
Published: July 2023

Abstract
Clear cell renal cell carcinoma (ccRCC) is known for its high heterogeneity and tendency to metastasize through the bloodstream, leading to limited treatment options and poor overall survival rates. To overcome these challenges, it is crucial to acquire a comprehensive understanding of the underlying biology of ccRCC. Therefore, we conducted a thorough analysis using single-cell RNA sequencing data obtained from samples of non-metastatic and metastatic ccRCC. Our analysis revealed significant differences in the composition of infiltrating immune cells within the primary tumor lesions between metastatic and non-metastatic ccRCC cases. Additionally, we identified two distinct tumor cell states, particularly proximal tubule cells, which exhibited significant enrichment in metastatic ccRCC cases. We found that MDK was highly expressed in metastatic ccRCC and exhibited significant prognostic value for patients. In metastaic ccRCC, we observed enhanced interactions between tumor cells and macrophages mediated by MDK, resulting in the polarization of macrophages towards an angiogenic and immune-suppressive M2-like phenotype. Furthermore, we observed notable differences in the interactions between macrophages and CD8 + T cells in non-metastatic and metastatic ccRCC. Metastatic ccRCC exhibited stronger interactions mediated by immune inhibitory molecules such as SPP1 and CD24, potentially contributing to immune suppression within tumor microenvironment. These dignificant findings provide valuable insights into the molecular and cellular signatures associated with metastatic ccRCC. Moreover, they open up promising opportunities for the development of novel biomarkers and therapeutic targets, specifically tailored to address the challenges posed by metastatic ccRCC.
Developing generic skills for future health professionals: Student and faculty perceptions of a Recovery College curriculum and courses
Authors: Zouaoui Inès , Drolet Marie-Josée , Briand Catherine 
Published: July 2023

Abstract
As healthcare systems worldwide grapple with complex challenges such as limited resources, qualified personnel shortages, and rapid technological advancements, there is an urgent need for educational transformation in healthcare professions. This urgency arises from the necessity for healthcare professionals to evolve beyond traditional roles and acquire essential generic skills such as adaptive, epistemic, relational, ethical, and citizenship skills - areas identified as gaps in conventional university curricula. This study investigates the potential of the Recovery College model, integrated into a Canadian university's healthcare curriculum, to address these gaps. Through qualitative group interviews with eight students and three faculty members, and subsequent descriptive content analysis, we explored the perceived outcomes of this model. We discerned 15 themes within the five core categories of generic skills (Epistemic, Ethical, Relational, Adaptative, and Citizenship skills), with "experiential knowledge acquisition" central to the training input, and other significant themes including "ethical sensitivity", "collaborative communication", "self-care", and "open-mindedness to diversity". Our findings highlight the Recovery College model's potential in fostering these crucial skills among future healthcare professionals and challenging prevailing epistemic injustices in healthcare. Further investigations are needed to understand the long-term effects of this model on healthcare practice and to explore its potential integration into wider healthcare education programs.