bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Associations between Acidosis and the Skeleton in Patients with Kidney Disease
Authors: Levy Rebecca V. , McMahon Donald J. , Agarwal Sanchita , Dempster David , Zhou Hua , Misof Barbara M. , Guo X.E. , Kamanda-Kosseh Mafo , Aponte Maria Alejandra , Reidy Kimberly , Kumar Juhi , Fusaro Maria , Brown Denver D. , Melamed Michal L. , Nickolas Thomas L. 
Published: February 2023

Significance Statement
Renal osteodystrophy (ROD) contributes substantially to morbidity in CKD, including increased fracture risk. Metabolic acidosis (MA) contributes to the development of ROD, but an up-to-date skeletal phenotype in CKD-associated acidosis has not been described. We comprehensively studied associations between acidosis and bone in patients with CKD using advanced methods to image the skeleton and analyze bone-tissue, along with biochemical testing. Cross-sectionally, acidosis was associated with higher markers of bone remodeling and female-specific impairments in cortical and trabecular bone quality. Prospectively, acidosis was associated with cortical expansion and trabecular microarchitectural deterioration. At the bone-tissue level, acidosis was associated with deficits in bone mineral content. Future work investigating acidosis correction on bone quality is warranted.

Background:
Renal osteodystrophy is a state of impaired bone quality and strength. Metabolic acidosis (MA) is associated with alterations in bone quality including remodeling, microarchitecture, and mineralization. No studies in patients with CKD have provided a comprehensive multimodal skeletal phenotype of MA. We aim to describe the structure and makeup of bone in patients with MA in the setting of CKD using biochemistry, noninvasive imaging, and histomorphometry.

Methods:
The retrospective cross-sectional analyses included 180 patients with CKD. MA was defined as bicarbonate ≤22 mEq/L. We evaluated circulating bone turnover markers and skeletal imaging with dual energy x-ray absorptiometry and high-resolution peripheral computed tomography. A subset of 54 participants had follow-up. We assessed associations between baseline and change in bicarbonate with change in bone outcomes. Histomorphometry, microCT, and quantitative backscatter electron microscopy assessed bone biopsy outcomes in 22 participants.

Results:
The mean age was 68±10 years, 54% of participants were male, and 55% were White. At baseline, acidotic subjects had higher markers of bone turnover, lower areal bone mineral density at the radius by dual energy x-ray absorptiometry, and lower cortical and trabecular volumetric bone mineral density and impaired trabecular microarchitecture. Over time, acidosis was associated with opposing cortical and trabecular effects: cortical expansion but trabecular deterioration. Bone-tissue analyses showed reduced tissue mineral density with increased heterogeneity of calcium distribution in acidotic participants.

Conclusions:
MA is associated with multiple impairments in bone quality. Future work should examine whether correction of acidosis improves bone quality and strength in patients with CKD.
Lenguaje y envejecimiento. Discusión acerca del deterioro/preservación de la sintaxis en los adultos mayores
Authors: Rivieri Eugenia Marta 
Published: February 2023

El estudio del procesamiento sintáctico en la vejez ha sido abordado, fundamentalmente a partir de dos enfoques: uno basado en la memoria y, otro basado en estructura y jerarquía. El primero vinculado a la memoria de trabajo y el segundo a la habilidad sintáctica. Las evidencias respecto de este procesamiento durante el envejecimiento son divergentes pues unas sostienen que la dificultad para procesar oraciones con complejidad sintáctica se debe a la disminución de la capacidad de almacenamiento y retención de la memoria de trabajo; mientras que otras sostienen que si bien se ralentiza el proceso, la habilidad sintáctica se preserva. El foco del fenómeno radica en que algunos autores ignoran en el procesamiento las distancias estructurales – nodos sintácticos- y solo consideran en la distancia sintáctica la dependencia de dos elementos (pronombre/referente). Esto abre un debate respecto de los sistemas de memoria a la base del procesamiento sintáctico. En este marco el objetivo de este artículo de reflexión es discutir estas dos aproximaciones teóricas e identificar las ventajas y limitaciones de cada uno de ellos a la hora de explicar el fenómeno.
Działalność Polskiego Stronnictwa Ludowego w Stanach Zjednoczonych Ameryki i Kanadzie (występującego pod nazwą Związek Przyjaciół Wsi Polskiej) w okresie prezesury Stanisława Mikołajczyka (1947–1966)
Authors: Turkowski Romuald 
Published: February 2023

Artykuł dotyczy działalności Polskiego Stronnictwa Ludowego, które ze względu na przepisy prawa amerykańskiego i kanadyjskiego nie mogło działać pod swoją nazwą. Funkcjonowało tam jako Związek Przyjaciół Wsi Polskiej. Organizacja ta kierowana przez grono polonijnych działaczy ludowych (Stanisław Mermel, Józef Migała, Bolesław Krakowski, Bolesław Biłogan, Tadeusz Paul, Feliks Rembiałkowski), przy wsparciu prezesa PSL Stanisława Mikołajczyka zdołała zorganizować kilkanaście kół PSL/Związku Przyjaciół Wsi Polskiej skupiających kilkuset członków i członkiń. Większość kół PSL/ZPWP działało w Chicago i okolicy. Udało się zorganizować koła PSL na wschodnim wybrzeżu i w kilku stanach środkowych. Koła PSL/ZPWP były oparciem politycznym dla przebywającego na stałe w Ameryce prezesa PSL S. Mikołajczyka. Poprzez swoich członków, którzy zdołali uzyskać dość wysoki status materialny, koła zorganizowały świetlice, domy ludowe oraz zespoły ludowe muzyczne i chóralne, co pozwoliło im zaistnieć w środowisku polonijnym. Koła wspierały linię polityczną S. Mikołajczyka, szczególnie w okresie licznych kryzysów wewnętrznych oraz w czasie ataków przeciwników na to ugrupowanie polityczne, które było spychane na margines emigracyjnej sceny politycznej. Na krótko pojawiło się też koło PSL/ZPWP na terenie Kanady. W artykule opisano działalność tej organizacji w okresie kierowania Naczelnym Komitetem Wykonawczym PSL przez S. Mikołajczyka. Organizacja ta przetrwała na terenie amerykańskim do końca lat 80. XX w. Poprzez zespół amerykański PSL/ZPWP dość wyraźnie oddziaływała na całe emigracyjne Stronnictwo.
Estimation of Phytochemicals and Antioxidant potency of Trigonella foenum-graecum Leaves (Variety HM444)
Authors: Moond Monika , Singh Sushila , Devi Ritu , . Pinki , . Rajita 
Published: February 2023

Background: Plants contain phytochemicals that are crucial to their growth and development. The potential health benefits of dietary phytochemicals including flavonoids, isoflavones and other polyphenols. Polyphenolic substances, the largest class of phytochemicals, act as strong antioxidants due to abundance of hydroxyl groups. Trigonella foenum-graceum also known as Fenugreek is rich source of phytochemicals, therefore possess extraordinary healing and medicinal properties. Methods: The present study is designed to evaluate chief phytochemical constituents and antioxidant capacity of acetone extract of dried Trigonella foenum-graecum leaves (Variety HM 444). The phytochemicals like total phenolic content by Folin-Ciocalteu method, total flavonoids by AlCl3 colorimetric assay, total sugars by method of Dubois and reducing sugars by method of Nelson as modified by Somogyi are determined in the acetone extract of leaves. The antioxidant capacity is determined by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and phosphomolybdenum assay. Result: Various phytochemicals such as total phenolic content (3.17 mg GAE/g), total flavonoids (1.34 mg CE/g), total sugars (32.35 mg/g), Reducing sugars (0.53 mg/g) and Non- reducing sugars (31.82 mg/g) are investigated in acetone extract of leaves. The antioxidant activity of leaves extract is evaluated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reported an IC50 value of 737.50 μg/mL. The total antioxidant capacity using phosphomolybdenum assay is 28.21 mg AAE/g.
A escola como heterotopia: aceitação e convivência entre bailarinos(as) e não bailarinos(as) em uma escola pública catarinense
Authors: Ambrósio-Accordi Andreia , Accordi Iury de Almeida 
Published: February 2023

How to conceive a school as a space of acceptance and coexistence between subjects who act in such different ways? Where do many no longer fit within the pre-established (hetero) norm that judges, sentences and punishes behavior outside of male / female binarism? The idea of a non-heteronormative school fits within the concept of heterotopy, a place or space that operates in non-hegemonic conditions. It is intended to describe situations in which students who have different forms of subjectivity assume a posture of acceptance and conviviality of their differences. A study is proposed using post-structuralist and Queer Theory assumptions, aiming to demonstrate how students (dancers) and non-dancers live in a non-heteronormative way. It was analyzed through ethnography how the students positioned themselves in relation to issues of heteronormative differences and the interactions between dancers and non-dancers students at different times and spaces in the school were described. The analyzed school can be considered a heterotopy, where most students accept each other and live together in order to respect their subjectivities.
An approach for prioritizing candidate genes from RNA-seq using preclinical cocaine self-administration datasets as a test case
Authors: Vannan Annika , Dell'Orco Michela , Perrone-Bizzozero Nora I. , Neisewander Janet L. , Wilson Melissa A. 
Published: February 2023

Abstract
RNA-sequencing (RNA-seq) technology has led to a surge of neuroscience research using animal models to probe the complex molecular mechanisms underlying brain function and behavior, including substance use disorders (SUDs). However, findings from rodent studies often fail to be translated into clinical treatments. Here, we developed a novel pipeline for narrowing candidate genes from preclinical studies by translational potential and demonstrated utility of this model in three RNA-seq studies of rodent self-administration. This pipeline uses evolutionary conservation and preferential expression of genes across brain tissues to prioritize candidate genes, increasing the translational utility of RNA-seq in model organisms. We found only 1 differentially-expressed gene (DEG) in any dataset after correcting for multiple testing (FDR < 0.05 or < 0.1), raising concerns about false positives and low statistical power that may impact these and other RNA-seq datasets. Thus, we demonstrate the utility of our prioritization pipeline using an uncorrected p-value. Curiously, even with an uncorrected p-value of 0.05 we saw low overlap of DEGs across the 3 selected datasets. Thus, we also advocate for improved RNA-seq data collection, statistical testing, and metadata reporting that will bolster the field’s ability to identify reliable candidate genes and compare results across studies.
Characterization of COVID-19 Vaccine Clinical Trial Discussions on the Social Question-and-Answer Site Quora
Authors: Xu Qing , McMann Tiana J. , Li Jiawei , Wenzel Christine , Mackey Tim Ken 
Published: February 2023

Abstract
Introduction:
Throughout the COVID-19 pandemic underserved populations, such as racial and ethnic minorities, were disproportionately impacted by illness, hospitalization, and death. Equity in clinical trials means that the participants in clinical trials represent the people who are most likely to have the health condition and need the treatment that the trial is testing. Infodemiology approaches examining user conversations on social media platforms have the potential to elucidate specific barriers and challenges related to clinical trial participation.
Methodology:
The study retrospectively collected and analyzed user question and answer posts from Quora in October 2021 using an inductive content coding approach. We also examined user’s publicly available profile metadata to identify racial and ethnic minority populations to capture their experiences, attitudes, topics, and barriers to COVID-19 vaccine trials.
Result
A total of 1,073 questions and 7,479 answers were collected based on structured automated keyword queries and data mining. A total of 763 questions and 2,548 answers were identified as related to COVID-19 vaccine clinical trials. The majority of these online interactions focused on asking questions and sharing knowledge and opinions about COVID-19 vaccine trials, including major topics related to: (a) interpreting whether clinical trial results could be trusted; (b) questions about vaccine efficacy and safety; (c) understanding trial design, regulatory considerations, and vaccine platforms; and (d) questions about trial enrollment, length, and adequate representation. Additionally, four major barriers discussed included: (i) disagreement from users regarding whether clinical trials require representation from different racial and ethnic minorities; (ii) concerns regarding the safety of trials when participating; (iii) lack of knowledge on how to register for a trial; and (iv) whether participants could withdraw from a trial to receive a more rapidly approved COVID-19 vaccine.
Conclusions
Our study found active user discussions related to COVID-19 vaccine clinical trials on Quora, including those specific to minority health topics and posted by self-identified racial and ethnic minority online users. Results from this study can help identify near real-time barriers to participation among underrepresented groups and support the design of future outreach strategies to help with recruitment and inclusive participation.
Childhood Trauma Questionnaire-Short Form: Evaluation of Factor Structure and Measurement Invariance
Authors: Cruz Daniel 
Published: February 2023

Abstract
Childhood trauma is known to put individuals at risk for mental and physical challenges later in life. Retrospective assessment of early abuse and neglect is critical for prevention and intervention efforts targeted at reducing the negative impacts of childhood trauma. The Childhood Trauma Questionnaire-Short Form (CTQ-SF) is a widely used measure to assess trauma among adolescents and adults, though there are some inconsistencies with regard to its factor structure and psychometric properties. The purpose of the current study was twofold: 1) to evaluate the hypothesized five-factor structure of CTQ-SF and 2) to test measurement invariance (equivalence) of the instrument across gender and race in a large, nationally representative sample of US adults (N = 863). Confirmatory factor analysis results indicate that the five-factor model fit the data well after some re-specification. The results also show that the five-factor model is generally invariant by gender and racial/ethnic groups and that the form’s subscales positively correlate with depression, anxiety, and abnormal inflammatory biomarker activity. Assessment is critical for the advocacy and treatment of individuals who have experienced abuse and neglect as children and adolescents. Our findings suggest that the CTQ-SF is a valuable tool for assessing childhood trauma and can be used in advocacy and treatment efforts.
a1,3-fucosylation of MEST promotes embryo implantation by activating translation initiation
Authors: Wang Hao , Cui Xinyuan , Wang Luyao , Fan Ningning , Qin Huamin , liu shuai , yan qiu 
Published: February 2023

Abstract
Embryonic trophoblast implanting into the uterus is the gateway for successful pregnancy. Dysfunctions of trophoblast cause pregnancy failure. Protein glycosylation plays crucial roles in reproduction process. However, it remains unclear if the glycosylation of trophoblasts involves in embryo implantation. By glycomics, proteomics combined with translatomics, our results revealed the that decreased α1,3-fucosylation, especially difucosylated Lewis Y (LeY) glycan, in the villus trophoblast of miscarriage patients compared with normal pregnancy women. Downregulating LeY by silencing key enzyme fucosyltransferase IV (FUT4) inhibited trophoblast implantation potential. Using proteomics analysis, we identified MEST scaffolding LeY at Asn163, and its expression was enhanced trophoblast implantation. We also provided novel evidence showing that decreased LeY modification on MEST dramatically hampered it binding with translation factor eIF4E2, and inhibited implantation-related gene translation initiation, which caused embryo implantation failure. The α1,3-fucosylation of MEST by FUT4 may serves as a new biomarker for evaluating the functional state of pregnancy and target for infertility treatment.
Ferroelectric hafnia as an ionic conductor
Authors: Zhao Guo-Dong , Liu Xingen , Xu Zhongshan , Ren Wei , Zhu Xiaona , Yu Shaofeng 
Published: February 2023

Abstract
The intensively concerned hafnia-based ferroelectric (FE) material has been controversial over whether the origin of its observed ferroelectricity being structural or electrochemical. We revisit the rigorous application of modern theory of polarization on displacive FE-HfO2, and make clear the microscopic mechanism of ionic conductance intertwined with continuous nucleation-and-growth FE switching in HfO2 from first principles. Independent from the involvement of vacancies, active oxygen ions in FE-HfO2 can be collectively conducted along continuous FE uniaxial-connected-paths (UCPs) in a typical nucleation-and-growth manner. The ionic conductance should have a nonlinear electric-field dependence from the Merz’s law, which is consistent with the strongly correlated ionic conductance. Based on our established physical picture, some abnormal experimental observations of HfO2 may be explained beyond the pristine understanding of FE switching within double-well potentials.
Detection and Mitigation of Malicious DDoS Floods in Software Defined Networks
Authors: Ahmad Furqan , Rehman Ubaid ur , Saleem Maham 
Published: February 2023

Abstract
Software-defined networking (SDN) has revolutionized network management by providing modular control and data plane attributes for flexible network management. It implies the concept of separating the control and data plane attributes for flexible network management. However, centralized management due to control plane separation in SDN also exposes it to cyber threats such as Distributed Denial-of-service (DDoS) attacks that can compromise the SDN controllers. In recent research, entropy-based attack detection approaches showed much significance among other detection methods but relying on entropy itself can neglect detection in several variables such as variations in flow specification. Based on these limitations, in this work, we have designed a DDoS attack detection framework inside the SDN control plane by integrating the packet flow initiation and its specifications properties with entropy-based algorithm to ensure correct measures of attack detection. The simulation is performed on Mininet network simulator, for implementing SDN architecture and the testbed is created on UDP flood attacks on commonly used data-centric tree topologies. Based on experimentation, this lightweight framework is designed to mitigate DDoS attacks by detecting its effects in the early stages to prevent SDN controller being hijacked due to immense packet flooding Based on the results, the proposed solution assures the SDN-based DDoS attack detection and mitigation under 150 packets maintaining significantly low detection time and high accuracy.accuracy.
Effective bioremediation of clarithromycin and diclofenac in wastewater by microbes and Arundo donax L.
Authors: Ercoli Laura , Rossetto Rudy , Giorgi Sabrina Di , Raffaelli Andrea , Nuti Marco , Pellegrino Elisa 
Published: February 2023

Abstract
Bioremediation of pharmaceuticals (PhCs) has gained large research efforts, but there is still a need to improve the performance of bioremediation systems by selecting effective organisms. In this study, we characterized the capability to remove clarithromycin (CLA) and diclofenac (DCF) by the bacterium Streptomyces rochei, and the fungi Phanerochaete chrysosporium and Trametes versicolor. Growth and content of the PhCs and a DCF metabolite (MET) by the energy crop Arundo donax L. were also evaluated under hydroponic conditions. The removal rate (RR) by S. rocheiincreased from 24 to 40% at 10 and 100 µg CLA L-1, respectively, averaged over incubation times. At 144 h, the RR by P. chrysosporium was 84%, while by T. versicolor was 70 and 45% at 10 and 100 CLA µg L-1. The RR by S. rochei did not exceed 30% at 1 mg DCF L-1 and reached 60% at 10 mg DCF L-1, whereas approached 95% and 63% by P. chrysosporium and T. versicolor, respectively, at both doses. Root biomass and length of A. donax were strongly affected at 100 µg CLA L-1. CLA concentration in roots and shoots increased with the increase of the dose and translocation factor (TF) was about 1. DCF severely affected both shoot fresh weight and root length at the highest dose and concentration in roots and shoots increased with the increase of the dose. DCF concentrations were 16-19 times higher in roots than in shoots and TF was about 0.1. MET was detected only in roots and its proportion over the parent compound decreased with the increase of the DCF dose. This study highlights the potential contribution of A. donax and the tested microbial inoculants for improving the effectiveness of bioremediation systems for CLA and DCF removal.
Comparing Classic and Genomic Selection for Iranian water buffaloes simulated population in the Khuzestan region for milk trait
Authors: Dezfouli Bahareh Taheri 
Published: February 2023

Abstract
The purpose of this study is to evaluate the cost-income and genetic progress in a simulated buffalo population by implementing classical and genomic selection programs with different ratios of artificial insemination. The target buffalo population was simulated based on the progressive process in 10 replications and a genome consisting of 4 chromosomes with 2500 markers and 100 QTLs, using QMSim software. A population with an effective size of 1500 animals was created in order to make a linkage disequilibrium between markers and QTLs. From the last generation of the base population, two populations were considered: buffalo cows with artificial insemination and those with natural mating. Five evaluation and selection methods were examined for 5 generations: progeny testing with 100% artificial insemination, 20% milk recording and 4 superior buffalo and genomic selection with 20, 50, 80 and 100% artificial insemination and 4, 5, 6 and 7 superior bulls. Comparing two genomic and classical evaluations with 100% artificial insemination, the results showed that genomic evaluation can enhance the genetic progress and also lower the average of inbreeding in the studied buffalo population. Also, comparing the cost of genomic evaluation programs with different levels of artificial insemination showed that increasing the level of artificial insemination elevated the program costs. The highest cost and income were obtained for the genomic selection program with 100% artificial insemination. Results showed that despite the high investment and maintenance cost, genomic evaluation is more economical in terms of profit than classical evaluation in the selection of buffalo bulls.