bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic use by poultry farmers in Kiambu County, Kenya: exploring practices and drivers of potential overuse
Authors: Kariuki Jeniffer Waiyego , Jacobs Jan , Ngogang Marie Paule , Howland Olivia 
Published: January 2023

Abstract
Background
Antibiotic resistance is a global concern threatening achievements in health care since the discovery of antibiotics. In Kenya, this topic remains understudied in a context of rising demand for livestock products, intensification and the concomitant increase in antibiotic use. Our study investigates drivers and practices of antibiotic use in poultry farming. The study was conducted in Kiambu County, Kenya.

Methods
A qualitative research methodology was employed: fourteen key informant interviews, twenty in-depth interviews, and four focus group discussions were undertaken. The interviews were semi-structured. Themes and subthemes from the interviews were generated through inductive analysis.

Findings
Of the farmers interviewed, sixty eight percent were female, thirty three percent of the sampled farmers could not read, and the majority (eight five percent) of farmers had reared poultry for at least ten years. Research findings showed that farmers extensively used antibiotics. Antibiotic use was influenced by factors such as high disease burden, access to medicines and economic pressure. Common practices included prophylactic use, use of antibiotics to enhance production, self-prescription use, use of combination antibiotics (A combination antibiotic is one in which two or more antibiotics are added together for additional therapeutic effect.), and antibiotics classified as critically important in human medicine. Key information sources for the farmers were agro- veterinary dispensers, sellers of day-old chicks, and peer-learning. External factors driving the inappropriate use of antibiotics included access to the antibiotics, influence by marketers such as sellers of day-old chicks, and branding. Use of antibiotics was also driven by economic factors among the farmers, sellers of day-old chicks and agro-veterinary dispensers.

Conclusions
Our findings indicate widespread use of antibiotics among poultry farmers in our study site. The use of antibiotics is influenced by an interplay of issues at the farmers’ level as well as broader social, economic and structural level factors. A multifaceted One Health approach focusing on regulatory frameworks, knowledge transfer, and research is required to promote stewardship and judicious use of antibiotics.
A pseudo-outbreak of MRSA due to laboratory contamination related to MRSA carriage of a laboratory staff member
Authors: Houkes Karlijn M. G. , Stohr Joep J. J. M. , Gast Karin B. , Couderé Karen , Weterings Veronica , Mutsaers - van Oudheusden Anne , Buiting Anton G. M. , Verweij Jaco J. 
Published: January 2023

Abstract
Background
Methicillin resistant Staphylococcus aureus (MRSA) is a major burden for hospitals globally. However, in the Netherlands, the MRSA prevalence is relatively low due to the ‘search and destroy’ policy. Routine multiple-locus variable-number of tandem repeat analysis (MLVA) of MRSA isolates supports outbreak detection. However, whole genome multiple locus sequence typing (wgMLST) is superior to MLVA in identifying (pseudo-)outbreaks with MRSA. The present study describes a pseudo-outbreak of MRSA at the bacteriology laboratory of a large Dutch teaching hospital.

Methods
All staff members of the bacteriology laboratory of the Elisabeth-TweeSteden hospital were screened for MRSA carriage, after a laboratory contamination with MRSA was suspected. Clonal relatedness between the index isolate and the MRSA isolates from laboratory staff members and all previous MRSA isolates from the Elisabeth-TweeSteden hospital with the same MLVA-type as the index case was examined based on wgMLST using whole genome sequencing.

Results
One of the staff members was identified as the probable source of the laboratory contamination, because of carriage of a MRSA possessing the same MLVA-type as the index case. Eleven other isolates with the same molecular characteristics were found in the database, of which seven were retrospectively suspected of contamination. Clonal relatedness was found between ten isolates, including the isolate found in the staff member and the MRSA found in the index patient with a maximum of eleven alleles difference. All isolates were epidemiologically linked through the laboratory staff member, who had worked on all these cultures.

Conclusions
The present study describes a MRSA pseudo-outbreak over a 2.5-year period due to laboratory contamination caused by a MRSA carrying laboratory staff member involving nine patients. In case of unexpected bacteriological findings, the possibility of a laboratory contamination should be considered.
Cysteine dioxygenase and taurine are essential for embryo implantation by involving in E2-ERα and P4-PR signaling in mouse
Authors: Zhang Di , Wang Zhijuan , Luo Xuan , Guo Hongzhou , Qiu Guobin , Gong Yuneng , Gao Hongxu , Cui Sheng 
Published: January 2023

Abstract
Background
Taurine performs multiple physiological functions, and the maintenance of taurine level for most mammals relies on active uptake from diet and endogenous taurine synthesis through its synthesis enzymes, including cysteine dioxygenase (CDO). In addition, uterus tissue and uterus fluid are rich in taurine, and taurine synthesis is regulated by estrogen (E2) and progesterone (P4), the key hormones priming embryo-uterine crosstalk during embryo implantation, but the functional interactions and mechanisms among which are largely unknown. The present study was thus proposed to identify the effects of CDO and taurine on embryo implantation and related mechanisms by using Cdo knockout (KO) and ovariectomy (OVX) mouse models.

Results
The uterine CDO expression was assayed from the first day of plugging (d 1) to d 8 and the results showed that CDO expression level increased from d 1 to d 4, followed by a significant decline on d 5 and persisted to d 8, which was highly correlated with serum and uterine taurine levels, and serum P4 concentration. Next, Cdo KO mouse was established by CRISPER/Cas9. It was showed that Cdo deletion sharply decreased the taurine levels both in serum and uterus tissue, causing implantation defects and severe subfertility. However, the implantation defects in Cdo KO mice were partly rescued by the taurine supplementation. In addition, Cdo deletion led to a sharp decrease in the expressions of P4 receptor (PR) and its responsive genes Ihh, Hoxa10 and Hand2. Although the expression of uterine estrogen receptor (ERα) had no significant change, the levels of ERα induced genes (Muc1, Ltf) during the implantation window were upregulated after Cdo deletion. These accompanied by the suppression of stroma cell proliferation. Meanwhile, E2 inhibited CDO expression through ERα and P4 upregulated CDO expression through PR.

Conclusion
The present study firstly demonstrates that taurine and CDO play prominent roles in uterine receptivity and embryo implantation by involving in E2-ERα and P4-PR signaling. These are crucial for our understanding the mechanism of embryo implantation, and infer that taurine is a potential agent for improving reproductive efficiency of livestock industry and reproductive medicine.
Identifying the shift in global wildfire weather conditions over the past four decades: an analysis based on change-points and long-term trends
Authors: Shi Ke , Touge Yoshiya 
Published: January 2023

AbstractMost of studies on change-point at a regional or global scale have only examined a single hydrometeorological variable and have been unable to identify any underlying explanations. In this study, we identified change-points and long-term trends of six wildfire-related variables and attempted to explain the cause of change-point from atmospheric–oceanic indices. As a result, we discovered that the main change-point dates for the precipitation, temperature, and drought codes, as well as the duff moisture code, fine fuel moisture code, and fire weather index, were 1995–2000 and 2000–2005, respectively. Furthermore, the relationship between the change-point of six variables and atmospheric–oceanic indices was discussed through the correlation coefficient. For example, the Atlantic Multidecadal Oscillation was found to dominate the precipitation in West Africa. In addition, we divided the globe into eight homogenous wildfire weather zones based on the change-point dates and long-term trends of the six variables.
Unprecedented warming in Northwestern India during April of 2022: roles of local forcing and atmospheric Rossby wave
Authors: Qin Jianhuang , Liu Heng , Li Baosheng 
Published: January 2023

AbstractA high surface air temperature (SAT) record over Northwestern India was reported in April 2022. This study examines the contribution of interannual variability on Indian SAT and possible reasons for the extreme SAT during April 2022. Result shows that the interannual variability of SAT is captured by the first two leading modes using the empirical orthogonal function (EOF) analysis. Both of them show highest values over the Northwestern India and are simultaneous energetic during 2022. The EOF1 is related to the Indian Ocean Basin Mode (IOBM) and an anomalous anti-cyclone in the troposphere, while the Rossby wave train from the North Atlantic to the north of India controls the EOF2. The active IOBM and strong Rossby wave source account for the extreme SAT over northwestern India during April 2022. In addition, the Indian regional mean SAT during April is well represented by the indices of IOBM and tropospheric anomalous anti-cyclone, which can help to improve the prediction of SAT over India.
Self-harm emergency department visits in Canada during the COVID-19 pandemic: evidence from a sentinel surveillance system
Authors: Saxena Shikha , Liu Li , Pollock Nathaniel , McFaull Steven R. 
Published: January 2023

Abstract
Background
Self-harm is a leading cause of morbidity and mortality globally, though the prevalence tends to be highest among adolescents. As an indicator in suicide surveillance, the incidence of self-harm is useful because it is sensitive to social, environmental, and economic conditions. During the COVID-19 pandemic, the epidemiology of self-harm has varied across contexts. This study aims to investigate the changes in self-harm emergency department visits in 2020 compared to a pre-pandemic period in 2018–2019.

Methods
Self-harm emergency department visits were extracted from the Canadian Hospitals Injury Reporting and Prevention Program database from 2018 to 2020. We compared the data in 2020 with the pre-pandemic baseline in 2018–2019. We examined the changes in volume, the percentages of self-harm cases among all intentional injuries by sex, age group, and the proportions of self-harm cases by method of injury. We also quantified the time trends of the percentages of self-harm cases among all intentional injuries using Joinpoint regression.

Results
The overall volume of emergency visits related to self-harm was higher in 2020 during weeks 24–51 compared to the average volumes for the same weeks of 2018–2019. Percentage of self-harm among all intentional injury emergency department visits was significantly higher by 6.1% among females (p < 0.05) and by 5.3% among males in 2020 than in 2018–2019 (p < 0.05). The 11-to-18-year age group showed an increase in the percentage of self-harm among all intentional injury emergency department visits by 7.4% in 2020 when compared to 2018–2019. Time trend analyses showed that the percentages of self-harm among all intentional injury emergency department visits were higher during weeks 4–52 in 2020 than in 2018–2019, for both males and females.

Conclusions
The percentage of emergency department visits related to self-harm among all intentional injury visits were higher during 2020 than in 2018–2019. These results underscore the importance of continued surveillance of self-harm in Canada to better understand the sociodemographic factors affecting self-harm and to inform the prevention strategies and policies.
Reply to comment by Williams on “spatial changes in inclusion band spacing as an indicator of temporal changes in slow slip and tremor recurrence intervals”
Authors: Nishiyama Naoki 
Published: January 2023

AbstractOur recent paper (Nishiyama et al. in Earth Planets Space 73:126, 2021) suggested that the sealing time of a crack-seal event recorded in quartz-filled shear veins in the Makimine mélange may temporally increase or decrease. Nishiyama et al. (Earth Planets Space 73:126, 2021) describes the optical estimates of the vapor–liquid ratio of primary two-phase fluid inclusions between solid inclusion bands in shear veins. The variation in vapor–liquid ratio was used as an indicator of fluid pressure conditions at the time of trapping of fluid inclusions during a crack-seal event. Comment on our paper (Williams in Earth Plantes Space 10.1186/s40623-022-01599-1 2023) raised the issue that our paper neglected the error in fluid pressure associated with the uncertainties in the optical estimate of the vapor–liquid ratio of fluid inclusions. Williams (Earth Plantes Space 2023 10.1186/s40623-022-01599-1) claimed that, if a certain uncertainty in the optical estimate of the vapor–liquid ratio is considered, there is a large error in the fluid pressure. The argument by Williams (Earth Plantes Space 2023 10.1186/s40623-022-01599-1) is based on the assumption that the uncertainty of the vapor–liquid ratio can be determined accurately. An accurate estimate of the uncertainty of the vapor–liquid ratio is possible if the homogenization temperature of the fluid inclusion is measured. However, it has been a challenging issue to measure the homogenization temperature of fluid inclusions between inclusion bands in a crack-seal vein, because the spacing of inclusion bands is too small (typically, a few tens of microns or less) to measure the homogenization temperature. Therefore, it is difficult to provide a confidential discussion regarding the error in the fluid pressure during a crack-seal event. Nishiyama et al. (Earth Planets 73:126, 2021) showed the sealing time of a crack-seal vein for a wide range of fluid pressure drops and discussed that the sealing time is comparable to the slow slip and tremor recurrence intervals when the fluid pressure drop is large. We consider that such a presentation and discussion are appropriate rather than discussing the error in fluid pressure based on the ambiguous uncertainty in the optical vapor–liquid ratio estimate.
Graphical Abstract
Research on interaction of innovation spillovers in the AI, Fin-Tech, and IoT industries: considering structural changes accelerated by COVID-19
Authors: Ho Chi-Ming 
Published: January 2023

AbstractThis paper aims to probe the influence of innovation spillovers in the artificial intelligence (AI) and financial technology (Fin-tech) industries on the value of the internet of things (IoT) companies. Python was utilized to download public information from Yahoo Finance, and then the GARCH model was used to extract the fluctuations of cross-industry innovation spillovers. Next, the Fama–French three-factor model was used to explore the interactive changes between variables. The panel data regression analysis indicates that the more firms accept innovation spillovers from other industries, the better the excess return; however, this effect differs because of industrial attributes and the environmental changes induced by COVID-19. Additionally, this study finds that investing in large-cap growth stocks of IoT firms is more likely to yield excess returns. Finally, the study yields lessons for policy leverage to accelerate the upgrading and transformation of innovation-interactive industries by referring to the practices of Singapore and South Korea.
Ecological validity of don’t remember and don’t know for distinguishing accessibility- versus availability-based retrieval failures in older and younger adults: knowledge for news events
Authors: Umanath Sharda , Coane Jennifer H. , Huff Mark J. , Cimenian Tamar , Chang Kai 
Published: January 2023

AbstractWith pursuit of incremental progress and generalizability of findings in mind, we examined a possible boundary for older and younger adults’ metacognitive distinction between what is not stored in memory versus merely inaccessible with materials that are not process pure to knowledge or events: information regarding news events. Participants were asked questions about public events such as celebrity news, tragedies, and political events that were widely experienced in the previous 10–12 years, responding “I don’t know” (DK) or “I don’t remember” (DR) when retrieval failed. Memories of these events are relatively recently acquired in rich, naturalistic contexts and are likely not fully separated from episodic details. When retrieval failed, DR items were recognized with higher accuracy than DK items, both immediately and 2 years later, confirming that self-reportednot rememberingreflects failures of accessibility, whereasnot knowingbetter captures a lack of availability. In fact, older adults distinguished between the causes of retrieval failures more precisely than younger adults. Together, these findings advance the reliability, validity, and generalizability of using DR and DK as a metacognitive tool to address the phenomenological experience and behavioral consequences of retrieval failures of information that contains both semantic and episodic features. Implications for metacognition in aging and related constructs like familiarity, remembering, and knowing are discussed.
Reliability and efficiency enhancement of a radial distribution system through value-based auto-recloser placement and network remodelling
Authors: Ghosh Bratati , Chakraborty Ajoy Kumar , Bhowmik Arup Ratan 
Published: January 2023

AbstractThe electric distribution system (EDS) is prone to faults leading to power interruptions. The present energy market demands that electricity utilities invest more in different measures to improve the performance of the EDS. The approach proposed here details a composite dual-phased methodology to improve the reliability and efficiency of the power delivered by the EDS. In the first phase, the optimal allocation of auto-reclosers (AR) is undertaken by employing a newly formulated algorithm. The determination of the total number and location for AR placement is based on the economic analysis of two factors, i.e., AR investment-maintenance cost and total benefit earned in terms of reliability improvement due to AR placement. The analysis also takes into account the impact of power outages on different load types, the load growth rate, and the inflation rate. Further, to enhance the efficiency of the AR-incorporated EDS, the technique of Radial Distribution System Remodelling is employed in the second phase. This method searches for a radial configuration that delivers power at minimum line losses. These phases comprising complex combinatorial operations are aided by a fresh hybrid of the Sine Cosine Algorithm, Krill Herd Algorithm, and a genetic operator of Differential Evolution. The results obtained from its application on the IEEE 69-bus distribution test system prove the credibility of the suggested formulation.
Post-traumatic epilepsy in Cameroon: a retrospective study in a referral hospital
Authors: Motah Mathieu , Gams Massi Daniel , Assonfack Sinju Franklin , Magnerou Annick Mélanie , Bila Lamou Eric Guemekane , Nkouonlack Cyrille , Doumbe Jacques , Esene Ignatius , Siysi Verla Vincent , Eyenga Victor-Claude , Mapoure Yacouba Njankouo 
Published: January 2023

Abstract
Background
Post-traumatic epilepsy is defined as the onset of at least one seizure beyond the first week following a traumatic brain injury (TBI). High prevalence of TBI in our setting may contribute to the burden of epilepsy in adult population. This is a retrospective review of medical records of patients admitted from January 1st, 2010 to December 31st, 2019) at Douala General Hospital. We included patients aged ≥ 18 years with seizure onset at least one week after TBI. Incomplete files and previously known epilepsy were excluded. Data on sociodemography, clinical and para-clinical features, treatment and outcome were analysed using R software version 36.2.

Results
We finally included 65 patients with post-traumatic epilepsy among 993 medical records of epilepsy. The mean age was 35.1 ± 12.6 years, with 64.6% of male. Road traffic accident was the main aetiology of brain trauma (78.5%), resulting in haemorrhagic contusions (21.5%), sub-dural haematoma (15.4%), and diffuse axonal lesions (15.4%) mainly. Seizure onset was within 2 years post-trauma in 73.8% of cases. Generalized tonic–clonic seizures were the commonest seizure’s type. Electroencephalogram was abnormal in 81%, including 47% of focal discharges. Antiepileptic drugs were mainly sodium valproate, carbamazepine, and phenobarbital. Seizure freedom was obtained in 67.7% of cases.

Conclusions
Post-traumatic epilepsy is a heterogeneous, frequent and often disabling complication of traumatic brain injury. Road traffic accident is the main cause of brain trauma. It affects a young and active population. About half of cases presented GTCS. With antiepileptic drugs, more than two-thirds of patients become seizure-free.
Stroke-related early seizures: clinical and neurophysiological study in a sample of Egyptian population
Authors: Mohamed Aya Taha , El Rakawy Mahmoud Hemeda , Abdelhamid Yousry Abo Elnaga , Hazzou Ahmed Mohammed , Wahid el din Mona Mokhtar 
Published: January 2023

Abstract
Background
Available data collected from patients of different types of acute cerebrovascular strokes can detect risk factors, clinical data (including semiology of seizures), EEG findings and functional outcome of these patients. Stroke, both ischemic and hemorrhagic, has been considered an essential cause of epilepsy, especially in the elderly. Complications of acute stroke during the early days after the insult determine the ultimate outcome of events. The aim of this study is to determine the clinical and neurophysiological characteristics of stroke patients with or without clinical evident fits for early detection and better management.

Results
The brain imaging of the cases group who developed seizures showed that 50% of the cases have arterial ischemic infarction, 30% venous infarction, 6.67% ischemic infarction with haemorrhagic transformation, 6.67% intracerebral haemorrhage and 6.67% have subarachnoid haemorrhage. Based on the 2017 ILAE criteria, 43.33% of the patients developed focal to bilateral tonic clonic seizures, 33.33% developed focal aware seizures, 16.67% of the patients developed generalized tonic clonic seizures and 6.67% of the patient developed status epilepticus. EEG findings of the group of patients who developed seizures showed, focal slowing in 46.67%, focal epileptiform activity in 13.33%, focal activity with secondary generalization in 10%, PLEDS in 6.67%, generalized epileptiform activity in 6.67%, generalized slowing in 6.67% and normal EEG in 10% of the patients. Non convulsive status was found in 2 patients (6.67%) of the group with altered mental status. There was no PLEDS in EEG of group of patients without clinical seizures. This study did not find age and sex differences in patients with and without seizures. In addition, it was found that there was no statistically significant difference between the three groups as regard history of diabetes mellitus, hypertension, heart diseases, atrial fibrillation, carotid stenosis and collagen diseases. There was no significant relationship between seizures and early treatment with Rtpa and thrombectomy.

Conclusions
Focal to bilateral tonic clonic and focal aware seizures were the most prevalent type of early onset seizures after stroke, followed by generalized tonic clonic seizures and status epilepticus. Most EEG findings in this study were focal slowing, focal epileptiform activities, generalized epileptiform activities and PLEDS.