bluuebunny/crossref_metadata_2025_split huggingface dataset:
Summary-data based mendelian randomization identifies gene expression regulatory polymorphisms associated with bovine paratuberculosis by modulation of the nuclear factor Kappa β (NF-κß)-mediated inflammatory response
Authors: Badia-Bringué Gerard , Canive Maria , Fernández Nora , Lavín José Luis , Casais Rosa , Blanco-Vázquez Cristina , Vázquez Patricia , Fernández Almudena , Bilbao Jose Ramón , Garrido Joseba M. , Juste Ramón A. , González-Recio Oscar , Alonso-Hearn Marta 
Published: January 2023

Abstract
Genome-wide association studies (GWAS) have identified host genetic variants associated with paratuberculosis (PTB) susceptibility. Most of the GWAS-identified SNPs are in non-coding regions. Connecting these non-coding variants and downstream affected genes is a challenge and, up to date, only a few functional mutations or expression quantitative loci (cis-eQTLs) associated with PTB susceptibility have been identified. In the current study, the associations between imputed whole-genome sequence genotypes and whole RNA-Sequencing data from peripheral blood (PB) and ileocecal valve (ICV) samples of Spanish Holstein cows (N= 16) were analyzed with TensorQTL. This approach allowed the identification of 88 and 37 cis-eQTLs regulating the expression levels of 90 and 37 genes in PB and ICV samples, respectively (FDR ≤ 0.05). Next, we applied summary-based data Mendelian randomization (SMR) to integrate the cis-eQTL dataset with GWAS data obtained from a cohort of 813 culled cattle that were classified according to the presence or absence of PTB-associated histopathological lesions in gut tissues. After multiple testing corrections (False discovery rate, FDR ≤ 0.05), we identified two novel cis-eQTLs affecting the expression of the early growth response factor 4(EGR4) and the bovine neuroblastoma breakpoint family member 6-like protein isoform 2 (MGC134040) that showed pleiotropic associations with the presence of multifocal and diffuse lesions in gut tissues; P = 0.002 and P = 0.017, respectively. While EGR4acts as a brake on T-cell proliferation and cytokine production through interaction with the nuclear factor Kappa β (NF-κß), MGC134040 is a target gene of NF-κß. Although no other genes were pleiotropically associated with PTB susceptibility after correction for multiple testing, several genes involved in splicing, innate immune response, apoptosis, blood coagulation, and regulation of epithelial cell adhesion were significantly associated with the presence of multifocal and diffuse lesions (nominal P-value ≤ 0.05). Our findings provide a better understanding of the genetic factors influencing PTB outcomes, confirm that the multifocal lesions are localized/confined lesions that have different underlying host genetics than the diffuse lesions, and highlight regulatory SNPs and regulated-gene targets to design future functional studies.
Water uptake of Haloxylon ammodendron in desert regions of Iran
Authors: Rahimabadi Pouyan Dehghan , Faghihi Vahideh , Azarnivand Hossein , Fasihi Javad , Malekian Arash , Yadollahzadeh Behzad 
Published: January 2023

Abstract
The complex relationships in the functioning of desert ecosystems and their environmental conditions can be expressed by the patterns of plant water use. In these areas, the water use sources of plants are a useful tool for resource management. Therefore, it is necessary to identify and track the available water resources and water use patterns. In this study, we investigated water use pattern of Haloxylon ammodendron growing in Semnan province, a semi-arid area of IRAN, using the stable isotope method. We used a simple, home-made Cryogenic Vacuum Distillation (CVD) system to direct extract the water in soil samples and different plant components before isotope analysis by mass spectrometer. The contribution of each possible water source of the plant xylem water was estimated using the IsoSource mixing model. The patterns in δ18O values in plant xylem water indicates the ability of H. ammodendron to use groundwater as the most important water resources in wet season to maintain its life. The results showed that there is a significant correlation between the isotope values with sand particles in the soil layers (P < 0.05). The results of this research can be of great help in strategic planting of H. ammodendron to prevent damages to groundwater resources and maintaining the long-term sustainability of groundwater.
Effect Of Shocks on Indian Stock Market
Authors: b Ashok , Vishwanath Akshay , Santosh Anadhu 
Published: January 2023

Abstract

The impact of major macro and micro economic factors (also known as shocks) on the Nifty 50 Index are studied. Various factors from interest rates, economic fallout and most recent events such as the covid pandemic and the Russian Ukraine war are considered. Results of this study reveal only the immediate impact on the benchmark index rather than the long-term effect.
Diet change and sustainability in Indigenous areas: Characteristics, drivers, and impacts of diet change in Gunayala, Panama
Authors: Lam Rodolfo Dam , Huynh Lam Thi Mai , Gasparatos Alexandros 
Published: January 2023

Abstract
Many Indigenous communities around the world have been experiencing rapid and profound diet changes. This Case Report uses a Sustainability Science lens to understand the characteristics of diet change in Indigenous Gunas communities of Panama, as well as its drivers and sustainability impacts. We observe the rapid westernization of diets mainly driven by modernization and development processes. However, this diet change has a series of intersecting sustainability impacts related to food security, health, and socio-cultural and environmental change. It is necessary to understand the intersection of these phenomena when designing programmes and interventions seeking to prevent or mitigate negative diet changes in Gunayala, and Indigenous contexts more broadly.
Quality of fish product fortified with guava peel powder as preserved in a prefabricated solar cooler **
Authors: Biswas Olipriya , Nanda Subhransu Mohan 
Published: January 2023

Abstract
In this work, guava (Psidium guajava L.) peel powder's antioxidant capacity and functional value in fish nuggets kept in a solar cooler at 5°C were examined. Guava powder peel was incorporated to fish nuggets at two distinct levels, 1.0%, and 1.5%, and the effect was compared to the control. High dietary fibre (56.93%), phenolics (43.99 mg GAE/g), and strong radical scavenging and reducing properties are observed in Guava powder. The addition of guava peel powder increased the emulsion stability and cooking yield and decreased the pH of emulsion. The incorporation of guava peel powder did not affect the amount of protein and ash content, but it increased the moisture content of fish nuggets. None of the textural qualities differed considerably except for springiness value. Guava peel powder was reported to prevent lipid peroxidation in fish nuggets during storage as determined by the TBARS level. The addition of guava peel powder decreased the growth of microbes due to its antimicrobial properties. Flavor and juiciness increased by the addition of guava peel powder in fish nuggets and can be employed as an antioxidant dietary fibre source in muscle foods.
Untargeted metabolomic study of lung cancer patients after surgery with curative intent: possible metabolites of good prognosis, intervention failure and recurrence
Authors: Sánchez-Espirilla Saida , Vega Antonio Pereira , Callejón-Leblic Belén , Díaz-Olivares Isabel , Santana-Martín Rafael , Rivera Carolina Gotera , Gómez-Ariza José Luis , López-Campos José Luis , Blanco-Orozco Ana Isabel , Seijo Luis , Rodríguez María , Fraysse Luis Padrón , Herrera-Chilla Ángela , Peces-Barba Germán , García-Barrera Tamara 
Published: January 2023

Abstract
Background
Lung cancer (LC) is a leading cause of mortality claiming more than 1.8 million deaths per year worldwide. Surgery is one of the most effective treatments when the disease is in early stages. The study of metabolic alterations after surgical intervention with curative intent could be used to assess the response to treatment or the detection of cancer recurrence.
Methods
In this study, we have evaluated the changes in the metabolomic profile in serum samples (n = 110) from preoperative (PRE) and postoperative (POST) LC patients collected at two different timepoints (1 month - A; 3–6 months - B) with respect to a group of healthy people. An untargeted metabolomic platform based on reversed phase (RP) and hydrophilic interaction chromatography (HILIC), both mounted into an ultra-high performance liquid chromatography (UHPLC) coupled to mass spectrometry (MS) was applied.
Results
A total of 33 altered metabolites belonging to carboxylic acids, organooxygen compounds, prenol lipids, fatty acyls, glycerolipids and glycerophospholipids classes were annotated comparing all the different studied groups. We found a group of metabolites altered in PRE (gabaculine, DG(14:0/22:1), stearamide, proline and E,e-carotene-3,3'-dione) whose levels returned to those of a baseline control group 3–6 months after surgery. Furthermore, humulinic acid A and 3-galactosyllactose levels may predict an unsuccessful surgery because they remained altered after the intervention in some patients. In addition, tranexamic acid could be studied more exhaustively to be proposed for monitoring the postoperative recurrence of the LC because its levels seem to be close to controls after one month of the intervention, but may differ significantly after 3–6 months.
Conclusions
This study provides unique insights into the metabolic profiles of LC patients after surgery at two different time points with a wide metabolic coverage combining complementary analytical methods.
Bilateral local primary parotid gland amyloidosis, a case report and literature review
Authors: Abdelwahed Mohammed , Kawam Alae , Rosca Oana C. 
Published: January 2023

Abstract
Amyloidosis of the salivary glands is a rare occurrence. It is an extremely uncommon manifestation of systemic amyloidosis and an even less common manifestation of primary amyloidosis. Here, we present a case of an 80-year-old female with a history of rheumatoid arthritis who presented with bilateral parotid masses and ear pain radiating to the shoulders. Fine needle aspiration revealed a cellular specimen with no atypical features, with abundant amorphous material. On Diff-Quick preparation the amorphous material showed a deep blue, while on Pap stain it was brightly eosinophilic. A cell block was prepared and demonstrated characteristic staining with Congo red. The material displayed the typical birefringence under polarized light microscopy. This case is an example of a rare presentation of amyloidosis and demonstrates the utility of fine needle aspiration (FNA) for the diagnosis of amyloidosis.
Non-targeted metabolomic analysis of highland barley original powder and enzymatic hydrolysis powder (pullulanase combined with α-glucosidase)
Authors: li yan , Sun Kangna , Song Xiaofan , Chen Fuzhang , Yuan Zhenzhen 
Published: January 2023

Abstract
Highland barley was used as raw material to prepare slow-digested starch by double-enzyme enzymatic hydrolysis process. The non-targeted metabolomics method was used to analyze the correlation between the differences in metabolites of small molecules before and after double-enzyme enzymatic hydrolysis, and to explore the effect on the metabolism of highland barley starch after double-enzyme enzymatic hydrolysis. UHPLC-QTOF-MS detection showed that the main up-regulated metabolites and metabolic pathways in enzymolysis group were mainly flavonoids, and the main down-regulated metabolites and metabolic pathways were mainly lipid metabolism. The results showed that compared with the control group, the enzymatic hydrolysis group regulated the content of sugars and flavonoids in the enzymatic hydrolysis starch by affecting the metabolic pathways such as glycolysis and flavonoid production, thereby affecting the starch characteristics of the double enzyme enzymatic hydrolysis of highland barley flour and making it produce a more gentle blood glucose response in the human body.
Materials for outer shell of 1.170 GWh (1.00669 kilo ton TNT) fusion device - weight basis
Authors: Rafique Muhammad Musaddique Ali 
Published: January 2023

Abstract
With recent developments in fusion engineering, interest has sparked in development of fusion devices for deterrent. Enormous amount of energy generated by combining two light nuclei could be contained and manipulated at will to trigger and accelerate micro explosions (from shock wave, x-rays or ion beam focusing) which finally result in full scale blast. Materials required to make such device are critical. They must possess high strength, high hardness, ductility, formability, drawability, and anisotropic properties. High entropy alloys (HEA) are new class of materials which nicely fulfils this requirement. Essentially, they are solid solutions of multi principal elements (usually > 5) eliminating the need of base metal as in conventional alloys. This gives them many unique properties which may be tailored at will (heat treatment, cold rolling, precipitation, irradiation). They also exhibit excellent directional properties with formation of distinct bands along certain preferred crystallographic planes even in hexagonal close packed structures. These anisotropic properties are strong function of rolling, working, or forging (swaging) direction and can be utilized to benefit. This study encompasses making outer shell of a typical fusion device selected on the basis of the weight, which is a function of area of pay load bay of carrier aircraft.
The expression and role of NADPH Oxidase 2 in colon cancer
Authors: Takiguchi Koichi , Shimizu Hiroki , Shoda Katsutoshi , Shiraishi Kensuke , Furuya Shinji , Hosomura Naohiro , Akaike Hidenori , Kawaguchi Yoshihiko , Amemiya Hidetake , Kawaida Hiromichi , Mochizuki Kunio , Kondo Tetsuo , Ichikawa Daisuke 
Published: January 2023

Abstract
Background
Nicotinamide adenine dinucleotide phosphate (NADPH) Oxidases (NOXs) are transmembrane proteins that generate reactive oxygen species. Recent studies have reported that NOXs play important roles in the progression of various cancers. However, the expression and role of NOX2 in colon cancer is unclear. Therefore, this study aimed to investigate the expression of NOX2 and effects on the prognosis of patients with colon cancer, and also examined the pathophysiological role of NOX2 in cell proliferation and motility.
Methods
We used 116 primary colon cancer samples obtained from patients who underwent curative resection for stage II or III colon cancer for immunohistochemistry to assess the relationship between NOX2 expression and clinicopathological factors and evaluate the prognostic significance of NOX2 expression in colon cancer patients. The expression levels of NOX2 protein and mRNA in human colon cancer cell lines were analyzed and two highly NOX2 expressed cell lines (HCT116 and RKO) were used for further study. Knockdown experiments with NOX2 siRNA were performed, and the effects on cell proliferation, cell cycle progression, migration, and invasion were analyzed.
Results
Immunohistochemical staining revealed that NOX2 protein was scarcely expressed in noncancerous tissue compared to cancer tissue, and 45 samples (38.8%) had positive staining for NOX2 expression in cancer tissue. No clinicopathological factor was significantly associated with NOX2 expression. The 5-year recurrence-free survival rate of the NOX2 positive group was significantly lower than that of the NOX2 negative group (61.1% vs. 79.3%, p = 0.029). NOX2 depletion using siRNA significantly inhibited cell proliferation, G1 to S phase cell cycle progression, migration, and invasion in the two cell lines.
Conclusions
NOX2 expression affects the prognosis of colon cancer patients, as well as the tumorigenesis of colon cancer cells. NOX2 may be a novel biomarker and therapeutic target for colon cancer patients.
Expression of hornerin in skin lesions of atopic dermatitis and skin diseases
Authors: Makino Teruhiko , Mizawa Megumi , Takemoto Keita , Shimizu Tadamichi 
Published: January 2023

Abstract
Cornification is an essential process that confers the skin barrier function. Among the proteins associated with cornification, filaggrin (FLG) plays an important role, and mutations in the FLG gene were shown to be an important predisposing factor for atopic dermatitis (AD). We previously identified an FLG-like protein, hornerin (HRNR). The exact function remains unknown; however, it is hypothesized that HRNR is associated with cornification. Recently, there have been several reports regarding a relationship between HRNR and the pathogenesis of AD. In the present study, we examined the HRNR expression in the skin lesions of 7 unrelated AD patients to clarify the role of HRNR in the pathogenesis of AD. Three of 7 AD specimens were obtained from erythematous lesions, suggesting an acute phase; the others were obtained from lichenified or pruriginous eruptions, suggesting a chronic phase. HRNR was detected in chronic AD lesions, although no signals of HRNR were observed in acute AD lesions. HRNR was detected in the cytokeratin6-expressing area, while Ki67-positive keratinocytes were more abundant in HRNR-positive epidermis. These findings suggest that the expression of HRNR may be associated with the hyperproliferation of epidermis in chronic AD lesions. We next examined the HRNR expression in skin diseases associated with hyperkeratosis, including ichthyosis vulgaris, epidermolytic ichthyosis, Darier’s disease, lichen planus, pustulosis et plantaris, actinic keratosis, and seborrheic keratosis. The expression of HRNR was decreased in ichthyosis vulgaris and was increased in lichen planus and pustulosis et plantaris, in parallel with the expression of FLG. In contrast, HRNR signals were irregularly observed in different cells from those expressing FLG in epidermolytic ichthyosis or actinic keratosis. The abnormal epidermal differentiation in these diseases may affect the unique expression of HRNR. Accordingly, HRNR may play an overlapping role with FLG in cornification; however, this unique expression pattern of HRNR in some diseases supports that HRNR may have a distinct role in the molecular processes of cornification.
Anatomical characteristics and morphometric analyses of the internal carotid artery using retrospective angiographic images
Authors: Kirisattayakul Woranan , Kiatkitkul Nukanda , Trakulsantirat Montita , Munkong Waranon , Pattum Panuwat , Iwanaga Joe , Tubbs R. Shane , Chaiyamoon Arada , Iamsaard Sitthichai 
Published: January 2023

Abstract
Purpose
Anatomical variations of the internal carotid artery (ICA) can cause complications during endovascular treatments. Therefore, the aim of this study was to investigate the features of the ICAs obtained from 2D digital subtraction angiography (2D DSA).
Methods
The morphometrics and angles of the ICA segments from 2D DSA images from a total of 100 patients (45 males and 55 females) were investigated. The lengths (L1-3), angles (A1-3), and diameters (D1-6) through the ICA measurement points (5 segments: C2 [petrous], C3 [lacerum], C4 [cavernous], C5 [clinoid], C6 (ophthalmic] were systemically recorded by two observers. All measured parameters were compared for both sexes and sides.
Results
The lengths (mm) and angles of the ICA were 7.20 ± 2.22 (L1), 15.71 ± 2.32 (L2), 10.99 ± 1.66 (L3) and 109.31 ± 17.77 (A1), 107.87 ± 20.51 (A2), 80.81 ± 16.33 (A3), respectively. There were no differences in the angulations of the A1-A3 segments between the sexes (p > 0.05). The L2 (C3-C4) was significantly longer in males, but only the left side of females showed a greater length (p < 0.05). The averaged mean diameters of the ICA (D1-6) in both sexes were 4.17 ± 0.55 mm. However, the diameters of the D1–D6 from left and right sides of males except the right D6 were significantly longer than those of females (p < 0.05). Also of significance was that the right diameters of females (D1) and males (D1 and D2) were shorter compared to left sides.
Conclusion
This study demonstrates some differences in lengths, diameters, and angles in both sexes and sides of the normal ICA. Neurosurgeons and neurointerventionalists should be aware of such variations when operating, performing interventional procedures, and interpreting 2D DSA.
Packaging materials and storage conditions affect nutritional quality of blended guava nectar
Authors: Singh Ajit Kumar , Sagar Vidya Ram , Rudra Shalini Gaur , Kumar Raju 
Published: January 2023

Abstract
The present study was carried out to elucidate the influence of packaging materials and storage conditions on changes in nutritional quality of blended guava and coloured grapes nectar (50:50) during 4 month of storage. blended juice was packaged in amber colour as well as transparent glass bottle followed by stored at low (6 ± 1°C) and ambient temperature ((28 ± 2°C)). Total soluble solid (TSS), titratable acidity (TA), reducing sugar (RS) and total sugar (TS), total phenolic content (TPC), total anthocyanin (TAC), ascorbic acid (AA), antioxidant activity (AOX) and sensory evolution were recorded in freshly prepared blended juice and during the 4 month of storage. the result showed that the TSS, acidity reducing and total sugar increased whereas antioxidant, ascorbic acid, phenolic and total anthocyanin content decreased during the storage with respective of packaging material. Loss of ascorbic acid, phenolic and total anthocyanin content were higher in blended nectar which was stored at ambient temperature as compared to low temperature. The ratio of 50:50 of guava and coloured grape blends is most acceptable for the preparation of anthocyanin rich guava nectar with maximum retention of anthocyanins, flavor and other nutrient.
Cyclic Feeding Regime Stimulates Chromatin Condensation in the Hepatocytes Nuclei of Old Rats
Authors: Danladi Ceasar Dubor , Serakinci Nedime , Ivanovich Bozhkov Anatolii , Girich Mishel 
Published: January 2023

Abstract
Aging related Human malignancies and diseases are linked to flaws in chromatin condensation, which may be due to its significance in the segregation of chromosome, regulation of genes and apoptosis. Investigations of CR (Calorie Restriction) have given a significant information on the mechanisms that control cell reactions to metabolic variations and how these structures function in synergy towards ensuring a harmonized reaction, chromatin plays a vital part in this process. CFR (Cyclic feeding regime) is more beneficial than CR in that it is easy to carry out. Therefore the reason for this investigation was to investigate the effect of CFR on chromatin condensation in the hepatocytes nuclei of 20 months old rats. DNA accessibility assay was used as a marker for chromatin condensation using Acridine Orange,DNA accessibility is the ability of A.O to intercalate and stain the DNA structure .Thus from the DNA accessibility results DNA condensation increased to 66% with 34% DNA accessibility after the first fasting with a reduction to 41% with 59% DNA accessibility following refeeding. The second fasting-refeeding regime lead to a sharp 74% increase and 35% decrease in DNA condensation with 26% and 65% DNA accessibility respectively. They results showed that old animals responded to CFR, with they consecutive fasting regime increasing the chromatin condensation.