bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of manure and biochar on the aluminum, copper, and iron bioaccumulation by Salicornia species in soil
Authors: Ashrafi Fahime , Heidari Ava , Farzam Mohammad , Karimi Alireza , Amini Malihe 
Published: January 2023

Abstract
Experiments were conducted to investigate the ability of two species of Salicornia persica and Salicornia perspolitana to extract heavy metals, including aluminum, iron, and copper. In this study, manure and biochar were applied to improve performance. The two species of S. persica and S. perspolitana were grown outdoor in experimental pots. The effect of experimental treatments including manure and biochar on the two species was investigated. The results showed that biochar significantly influenced (P < 0.05) the accumulation of heavy metals in both S. persica and S. perspolitana. In biochar treatment, the values of heavy metals in roots and shoots were found to be 3327 mg/kg, 109.17 mg/kg for aluminum, 3527 mg/kg and 142.17 mg/kg for iron, and 73.93 mg/kg and 11.90 mg/kg for copper, respectively. In the manure treatment, the values of heavy metals in the roots and shoots were reported as 3993 mg/kg and 119.29 mg/kg for aluminum, 5542 mg/kg and 179.17 mg/kg for iron, and 78.67 mg/kg and 12.74 mg/kg for copper, respectively. In general, our findings showed that using biochar and manure in the soil allowed heavy metals to be stabilized, which reduced heavy metal accumulation in both S. persica and S. perspolitana. Heavy metal accumulation was higher in S. perspolitana than in S. persica.
RNA Binding Protein-related bioinformatics analysis reveals that PCF11 is a prognostic biomarker in oral squamous cell carcinoma
Authors: Chen Gaoxiang , Song Yuxing , Zheng Zenan , Ai Yilong , Lv Xiaozhi 
Published: January 2023

Abstract
Background: RNA binding proteins (RBPs) play important roles in tumor carcinogenesis and progression. However, the underlying mechanisms of RBPs in oral squamous cell carcinoma (OSCC) are still unclear. This study was aimed to explore the prognostic value of RBP-related genes in OSCC.
Methods: Transcriptome and clinical data of OSCC were downloaded from The Cancer Genome Atlas (TCGA) database, and the differentially-expressed RBPs were screened via R software. Then, we constructed a risk model and preformed comprehensive bioinformatics analysis. After that, the prognostic related RBPs were screened out and verified by other cohorts from Gene Expression Omnibus (GEO) database subsequently. In the end, the target RBP, were investigated in OSCC cell lines and in 84 OSCC patients.
Results: A total of 249 differentially-expressd RBPs were screened out and PPI networks were established. 7 prognostic RBPs were obtained via Cox regression analysis. Furthermore, we successfully established a prognostic model and plotted a nomogram to visualize this model. Subsequently, 5 RBPs were selected out by survival analysis and only PCF11 was significantly differentially-expressed in OSCC after verifying them in GEO database. GSEA analysis showed that low expression of PCF11 was associated with the enhancement of oxidative phosphorylation(OXPHOS) and proteasome. In addition, PCR and immunohistochemical staining results revealed that PCF11 expression were down-regulated in OSCC cells and tissues. And the down-regulation of PCF11 was remarkably showed in advanced OSCC patients.
Conclusions: RBPs might be potential biomarkers in OSCC, provided a novel target for the diagnosis and therapy of OSCC.
Effect of Repeated Intravitreal anti-Vascular Endothelial Growth Factor Drugs on Corneal Endothelium
Authors: QI Yuan-Yuan , ZHANG Fan , LI Chun-Shi , JIN Cui-Hua , XUE Xin-Xia , CHU Meng , YI Ling-ling , Zhang LI-jun 
Published: January 2023

Abstract
Objective
To investigate the potential effect of repeated intravitreal injection of anti-Vascular Endothelial Growth factor(anti-VEGF)drugs on corneal endothelium in patients with macular edema.
Methods
This is a prospective study in which a total of 64 patients (64 eyes) were treated with intravitreal injection of anti-VEGF. There were 19 cases of neovascular Age-Related Macular Degeneration (nAMD), 20 cases of Diabetic Macular Edema (DME)and 25 cases of Retinal Vein Occlusion (RVO). 29 cases were treated with Aflibercept (2mg/0.05ml) and 35 cases with Ranibizumab (0.5mg /0.05ml). 3 + PRN therapy was used. Corneal endothelial microscopy was used to measure the corneal thickness and corneal endothelium. The various changes in corneal thickness, corneal endothelial cell density, average cell area, coefficient of variation, and proportion of hexagonal cells were compared between one month after each injection and before injection.
Results
The proportion of corneal endothelial hexagonal cells (Hex%) in the operative eyes after the 2nd and 3rd injection was found to be significantly lower than that of baseline. Hex% in the operative eyes after the 4th injection was lower than that of baseline, 2nd as well 3rd injection and the difference was statistically significant (P < 0.05). The number of injections was irrelevant to the changes of corneal endothelium. There were no statistically significant differences in all corneal endothelial indexes in contralateral eye.
Conclusion
Repeated intravitreal anti-VEGF drugs can affect the morphology of corneal endothelial cells. Thus, for patients with fundus disease who need multiple intravitreal injection of anti-VEGF drugs, attention should be paid to the changes of corneal endothelium, especially for those suffering with diabetes, a history of eye surgery (such as cataract surgery), or patients with high intraocular pressure and other diseases that can adversely affecting corneal endothelium.
Efficiency of Botulinum Toxin Injection into the Arm on Postural Balance and Gait after Stroke
Authors: Lee Junhee , Park Ji Eun , Kang Byung Heon , Yang Seung Nam 
Published: January 2023

Abstract
The purpose of this study was to clarify the association between improvement of spasticity in hemiplegic patient’s upper extremity with Botulinum toxin injection and improvement in postural balance and gait function. For this prospective cohort study, sixteen hemiplegic stroke patients with upper extremity spasticity were recruited. The plantar pressure with gait parameters, postural balance parameters, Modified Ashworth Scale, and Modified Tardieu Scale were evaluated before, 3weeks and 3months after Botulinum toxin A (BTxA) injection. Spasticity of hemiplegic upper extremity before, and after BTxA injection were significantly changed. Plantar pressure overload in affected side was reduced after BTxA injection. The mean X-speed and the horizontal distance decreased in postural balance analysis with eyes-opened test. Improvement in hemiplegic upper extremity spasticity showed positive correlation with gait parameters. In addition, improvement in hemiplegic upper extremity spasticity was positively correlated with change in balance parameters in postural balance analysis with eyes-closed and dynamic tests. This study focused on the effect of stroke patient’s hemiplegic upper extremity spasticity on their gait and balance parameters and identified that the BTxA injection on hemiplegic patient’s spastic upper extremity improve postural balance and gait function.
Oxidative stress and genotoxicity in oral epithelial cells of subjects undergoing Fixed Orthodontic Appliances
Authors: Ángeles-Estrada Lucía , Pérez-Soto Elvia , Pérez-Vielma Nadia Mabel , Gómez-López Modesto , Sánchez-Monroy Virginia 
Published: January 2023

Abstract
Objectives The aim of this study was to analyze changes in oxidative stress (OS) and the genotoxic damage in oral epithelial cells caused by fixed orthodontic appliances.
Materials and methods Samples of oral epithelial cells were obtained from fifty-one healthy voluntary subjects who had an indication for orthodontic treatment. The samples were obtained before and after 6- and 9-months starting treatment. OS was evaluated by quantitation of 8-hydroxy-2’deoxyguanosine (8-OHdG) and by relative gene expression of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT). DNA degradation and instability were evaluated by multiplex PCR and fragment analysis for human identification.
Results Quantitation of 8-OHdG evidenced increase during treatment, although not statistically significant. SOD showed increase of 2.5 and 2.6-fold after 6 and 9 moths of treatment. CAT shows an increase of 3-fold, after six months of treatment, while after 9 months of treatment the level expression decreased to level similar to that before treatment. DNA degradation was found in 8% and 12% of DNA samples after 6 and 9 months of treatment,respectively, while DNA instability was detected in only 2% and 8 % of DNA samples after 6 and 9 months of treatment, respectively.
Conclusions Results show light changes in OS and genotoxicity after fixed orthodontic appliances, but also suggesting a biological adaptation response to treatment after 6 months.
Clinical relevance OS in buccal cavity represents risk to oral and systemic diseases. Implementation of antioxidant supplementation, use of thermoplastic materials, and time reduction during orthodontic therapy may reduce the risk.
Microbial hazard identification and exposure assessment of raw milk consumption in Jimma zone, south west Ethiopia
Authors: Gume Beje , Berhanu Leykun , Kassa Tesfaye , Bediru Habib , Fikre Assegid Getaneh , Dadi Lelisa Sena , Mereta Seid Tiku 
Published: January 2023

Abstract
A cross-sectional study was carried out in Jimma zone, Southwest Ethiopia to evaluate the presence of five target pathogenic bacteria including Escherichia coli O157:H7, Salmonella enterica Typhimurium, Staphylococcus aureus, Listeria monocytogenes, and Campylobacter jejuni in raw milk and to assess the exposure level of consumers. Raw milk samples were analyzed in laboratory and semi-structured interview questions and observational checklists were administered to collect data on the amount and frequency of consumption. Statistical analysis of data obtained from laboratory results and questionnaire surveys were analyzed using Statistical Package for Social Science (SPSS) version 23. Among the total of 150 raw milk samples collected from the study area, about 61.3% were found to be contaminated by one or more types of pathogens along the dairy value chain. The highest and the least bacterial counts recorded from E. coli and L. monocytogenes contamination in the milk were 4.88 log10cfu/ml and 3.45 log10cfu/ml respectively; whereas Campylobacter jejuni were totally absent in the retailer outlets. The mean concentrations of all pathogens demonstrated significant statistical difference (p < 0.05) using 95% confidence interval where the prevalence percentage of isolates increased as milk transported from dairy farms to the retail outlets. All pathogens were detected in the unsatisfactory level along the dairy value chain, particularly at retail outlets, where raw milk is ready for consumption. The estimated mean annual risk of acquiring intoxication of E. coli across retailer outlets is 100% whereas salmonellosis, S. aureus intoxication, and listeriosis are 84%, 65% and 63% respectively. The results of our study, therefore, demonstrated that the high annual probability of infection could be ascribed to the traditional production and consumption patterns of raw milk in Ethiopia. Consuming such ready-to-eat foods with unacceptable microbiological quality is one of the concerns that develop public health risks that increase the chance of food-borne illness. Hence, it requires regular monitoring and implementing hazard identification and critical control point principles along raw milk production to retail points.
NIBAN2 promotes osteogenic differentiation and antagonizes osteoporosis by regulating RUNX2 alternative splicing
Authors: Zhang Sheng , Yang Zhiqiang , Wei Renxiong , Zhang Yufeng , Chen Zhe , Lv Xuan , Deng Zhouming , Huang Zan , Xie Yuanlong , Cai Lin 
Published: January 2023

Abstract
Osteoporosis is characterized by excessive bone resorption and/or defects in bone formation. The identification of factors promoting osteoblast differentiation provides potential targets for osteoporosis therapy. Through integral analyses of multiple datasets, we discovered that NIBAN2 was tightly associated with bone formation and osteoporosis. NIBAN2 promoted osteoblast differentiation in vitro, and conditional Niban2 knockout in osteoblasts caused bone loss and insufficient mineralization in mice. Transcriptomic and mass spectrometry analyses demonstrated that NIBAN2 decreased the expression of repressive Runx2 alternative splicing transcripts. NIBAN2 did so by interacting with the Hnrnpu-cored spliceosome complex and switch components. In vivo, Niban2 rescued bone loss in ovariectomy-induced osteoporosis in mice. Thus, our research provides a potential anabolic therapy target for osteoporosis involving regulation of alternative splicing.
Polar Solomon Rings in Ferroelectric Nanocrystals
Authors: Wang Jing , Liang Deshan , Ma Jing , Fan Yuanyuan , Ma Ji , Jafri Hasnain , Yang Huayu , Zhang Qinghua , Wang Yue , Guo Changqing , Dong Shouzhe , Liu Di , Wang Xueyun , Hong Jiawang , Zhang Nan , GU Lin , Yi Di , Zhang Jinxing , Lin Yuan-Hua , Chen Long-Qing , Huang Houbing , Nan Ce-Wen 
Published: January 2023

Abstract
Solomon rings, upholding the symbol of wisdom with profound historical roots, had been widely used as decoration in ancient architecture and clothes. Yet, it was only recently discovered that such topological structures can be formed by self-organization in biological/chemical molecules, liquid crystals, etc. Here, we report the observation of polar Solomon rings in a ferroelectric nanocrystal, which are composed of two interwoven vortices and mathematically equivalent to a 412 link in topology. By combining piezoresponse force microscopy observations and phase-field simulations, we demonstrate the reversible switching between polar Solomon rings and vertex textures by an electric field. The two types of topological polar textures exhibit distinct absorption of terahertz infrared waves, which can be utilized in infrared displays with a nanoscale resolution. Our study establishes, both experimentally and computationally, the existence and electric manipulation of polar Solomon rings, a new form of topological polar structures, which may provide a simple way for fast, robust, and high-resolution optoelectronic devices.
The role of I4.0 in a data-driven shared platform for a Circular product and service package
Authors: Atif Sehrish 
Published: January 2023

Abstract
This study aims to explore the role of Industry 4.0 (I4.0) technologies with the circular economy (CE) in the shadow of a Product Service System (PSS). Integrating innovative technologies creates a circular economic system that enhances the value and legitimacy of their trade. Recently, many global industries have shifted their focus towards product-based services to perpetuate in today’s competitive market without negatively influencing environmental detrition. This is the first study to highlight how the service-centric paradigm has a significant impact on the creation of circular products, the management of their lifecycles, and their cost analysis. Additionally, this study will advance our understanding of how I4.0 technologies use waste to create sustainable energy sources rather than consuming virgin resources (normally considered a linear model). This research used the Systematic Literature Review (SLR) methodology to analyse and categorise a literature survey of 126 research articles published over ten years (2013-2022). The analysis indicates that the early research is mostly focused on defining the key variables (CE, I4.0, and PSS) and developing various frameworks to promote eco-efficient services and/or sustainable development using I4.0 technologies and is engineering focused. It is established that two propositions leverage CE; the first one is value co-creation, which enhances perceived value. In contrast, the other one is related to the application of data-driven platforms using I4.0 technologies for sculpting the strategy and other decision support. There need to be more studies analysing CE, I4.0, and PSS. Therefore, this study has analysed the suitability of the theoretical propositions regarding the impact of applying I4.0 technologies with CE and PSS towards this paradigm shift.
High-Resolution Population Genetic Structure of Tawny Crazy Ant (Nylanderia fulva Mayr: Hymenoptera: Formicidae) from the Origin in South America and Introduced Regions of the United States
Authors: Holt Jocelyn R. , Lerma James Montoya , Calcaterra Luis A. , Raszick Tyler J. , Medina Raul F. 
Published: January 2023

Abstract
Background
The tawny crazy ant (Nylanderia fulva Mayr) is native to South America and was first reported in the continental United States (US) in 1938. It was not until the 1990s in Florida and 2000s in Texas that this ant was considered a serious pest in the US. Tawny crazy ant (TCA) is currently considered an invasive pest in six US states and this ant’s invasion success is attributed in part to a unicolonial nature, multiple queens per nest, natural enemies release in the invasive range, and ability to detoxify venom from other competitor ant species. A limited number of low-density molecular markers have previously shown little genetic differentiation among TCA populations across their geographic distribution in the US.
Results
Using High Throughput Sequencing (HTS) we obtained high-density molecular markers (i.e., SNPs) for TCA samples. With 26,657 SNPs we identified genetic variation among TCA populations in different states across the US (i.e., Texas, Louisiana, Alabama, Mississippi, Georgia, and Florida) and in South America (i.e., Argentina, Colombia, and Peru).
Conclusion
Our results underscore that for recently introduced invasive species, increasing the number of molecular markers used in population genetic studies can provide greater resolution. High-resolution information on regional genetic differences can help inform pest management strategies.
Plasma-derived exosomes contributes to Endothelial-to-mesenchymal transition in moyamoya disease
Authors: Liu Jilan , Chen Chao , Qin Xianyun , Lu Yan , Zhang Bin , Jin Feng 
Published: January 2023

Abstract
Moyamoya disease was a cerebrovascular disease with a high disability rate, and its pathogenesis was still unknown. Endothelium-mesenchymal transition (EndMT) was the pathological basis of many vascular diseases, however, whether EndMT played a key role in moyamoya disease has not been reported. Multiplex fluorescent immunohistochemistry staining confirmed that CD31, VE-cadherin and E-cadherin were down-regulated, α-SMA and Vimentin were significantly up-regulated in moyamoya vascular endothelial cells. Therefore, we proposed for the first time that EndMT may exist in the vessels of moyamoya disease. Plasma-derived exosomes (PDEs) can transmit information between cells and tissues and are of great value in many disease studies. PDEs significantly promoted cell proliferation and migration, and make cells slender. PDEs induced EndMT phenotype changes in cerebral vascular endothelial cells including decreased endothelial cell markers expression and increased mesenchymal cell markers expression. We demonstrate that EndMT phenotypic alterations are mediated in part by microRNA. Thus, we concluded that PDEs induce the EndMT phenotype to promote the development of moyamoya disease. This study aimed to provide a new theoretical basis for elucidating the pathogenesis of moyamoya disease.
How to measure snoring: A systematic review
Authors: Kim Su Geun , Cho Sung-Woo , Rhee Chae-Seo , Kim Jeong-Whun 
Published: January 2023

Abstract
Objective
Snoring is the most common and main symptom of obstructive sleep apnea. therefore, it should be managed effectively. Various methods of measuring snoring are available, and even if the measurement is performed the same way, communication is difficult because there are no common reference values between the researcher and clinician with regard to intensity and frequency, among other variables. In other words, no consensus regarding measurement has been reached. Therefore, this study aimed to review the literature related to the measurement of snoring, such as measurement devices, definitions, and device locations.
Methods
A literature search based on the PubMed, Cochrane, and Embase databases was conducted from the date of inception to April 30, 2022. Twenty-seven articles were included in this study. Papers that mentioned only the equipment used for measurement and did not include individual details were excluded from the study.
Results
Three representative methods for measuring snoring emerged. These include (1) a microphone, which measures snoring sound; (2) piezoelectric sensor, which measures snoring vibration; and (3) nasal transducer, which measures airflow. In addition, recent attempts have been made to measure snoring using smartphones and applications.
Conclusion
Numerous studies have investigated both obstructive sleep apnea and snoring. However, the methods of measuring snoring and snoring-related concepts vary across studies. To understand the pathophysiology underlying obstructive sleep apnea and address sleep-related diseases, consensus in the academic community on how to measure and define snoring is required.
The Effect of Coronary Artery Bypass Graft Operations on Serum Asprosin Values
Authors: Kafadar Huseyin , Guven Cengiz 
Published: January 2023

Abstract
Introduction:
The main purpose of this study was to investigate the effects of coronary artery bypass graft operations (CABGO) on serum asprosin levels.
Methods
Twenty-five patients who underwent CABGO with the diagnosis of coronary artery disease (CAD) were included in the study. The patients were divided into three groups according to the study time; preoperative (preop), postoperative 1st day (postop1), and postoperative 7th day (postop7). The effects of the operation on the asprosin levels were discussed in the light of the literature data by evaluating the fasting plasma asprosin levels for at least 8 hours from all three groups.
Results
There were 13 men and 12 women in the Study Group with a mean age of 64.4 ± 9.9. It was found that the asprosin levels that were measured in postop1 and postop7 groups were significantly higher than in the preop group (P < 0.001). When the factors that affected the asprosin hormone levels at different times were examined, it was found that only the gender factor was significant (P = 0.013). Although a significant increase was observed in postop7 and postop1 when compared to preop value in both genders, a decrease was detected in postop7 when compared to postop1; and although this decrease was statistically significant in women (P = 0.010), it was not significant in men (P = 0650).
Conclusion
In CABGO patients, increased serum asprosin levels are detected in the early postoperative period. This increase was more significant in women, which may be because of the protective effects of asprosin, which also has antioxidant features.