bluuebunny/crossref_metadata_2025_split huggingface dataset:
An efficient and reproducible Agrobacterium-mediated genetic transformation method for the ornamental monocotyledonous plant Ornithogalum dubium Houtt.
Authors: Tripathi Pankaj Kumar , Ayzenshtat Dana , Kumar Manoj , Zemach Hanita , Yedidia Iris , Bocobza Samuel 
Published: March 2023

Abstract
Ornithogalum is a genus from the Hyacinthaceae (Asparagaceae) family that comprises about 200 species with remarkable white, yellow, or orange flowers that display exceptional vase life. These properties have made it a popular cut flower and pot plant. Forward genetics approaches may be advantageous to generate novel phenotypes, but the Agrobacterium-mediated transformation of plants from this genus remains challenging. Here, a stable and efficient Agrobacterium-mediated transformation system was established for O. dubium. We found that the timing of transformation with respect to light exposure of the tissue affected transformation rates more than other tested parameters. In the transgenic plants obtained, T-DNA integrations were confirmed by polymerase chain reactions, and positive plants were established in the greenhouse and displayed weak transgene expression. This study exposed an efficient platform for gene function research and germplasm improvement in O. dubium plants. The present protocol is now available for the development of novel improved O. dubium varieties.
The use of Palliative Performance Scale as the Sole Prognostication Tool among Patients Transferred Under Palliative Care: A Single Institution’s Experience in Qatar
Authors: Abdullah Hodan , Allam Ayman , Singh Kalpana , keldi Shaikhah Al , Idris Zeinab , Hassan Azza , Lenjawi Badriya Al 
Published: March 2023

Abstract
Background
The demand for cancer palliative care services in Qatar is increasing due to the increase in the number of people with advanced cancer who require supportive measures and end of life care. End-of-life prognostication is a vital step to determine survival; however, this is extremely challenging, and requires precise tools besides clinicians’ judgments. The palliative performance scale (PPS) tool is used to prognosticate survival and to assess patients ‘symptoms at the National Centre for Cancer Care and Research (NCCCR). The aim is to analyse the correlation between PPS and overall survival (OS) and to report the experience of using PPS as a sole prognostication tool among patients transferred under palliative care at NCCCR in Qatar.
Method
This is a retrospective cohort study. Data of advanced cancer patients who were accepted under the palliative care program were retrieved from the electronic medical record from January 1, 2017 until December 31, 2021, to test the accuracy of PPS in estimating the prognosis and survival time. The PPS scores were compared with the dates of death for each patient.
Result
The findings revealed that the initial PPS is a significant predictor for overall survival, along with the type of cancer, but not with age or gender. There are clinical differences between PPS scores of ≤ 30% and those of 40% or more; therefore, PPS ≤ 30% was chosen as a cut-off value in this present study. The results revealed a statistically significant higher OS for patients with PPS of 40–80% compared to those with PPS of ≤ 30% (p = 0.03).
Conclusion
There is a need to couple PPS with other prognostication tools to achieve accuracy in predicting overall survival time for cancer patients under palliative care.
Prospective Validation of the Venous Excess Ultrasound “(VExUS)” Score
Authors: Longino August , Martin Katharine , Leyba Katarina , Siegel Gabriel , Gill Edward , Douglas Ivor , Burke Joseph 
Published: March 2023

Abstract
Venous congestion is an under-recognized contributor to mortality in critically ill patients. Unfortunately, venous congestion is difficult to measure, and right heart catheterization (RHC) remains the gold standard. Recently, a novel “Venous Excess Ultrasound (VExUS)” score was developed to noninvasively quantify venous congestion using inferior vena cava (IVC) diameter and doppler flow through the hepatic, portal, and renal veins. A preliminary retrospective study of post-cardiac surgery patients showed promising results, including a high positive-likelihood ratio of high VExUS grade for acute kidney injury. However, further validation studies have not been reported in broader patient populations, and validity of VExUS as compared to other measures of venous congestion such as IVC diameter is unknown. To address this gap, the authors prospectively validated the VExUS against right atrial pressure (RAP), with comparison to inferior vena cava (IVC) diameter. Patients undergoing RHC at the Denver Health Medical Center underwent VExUS examination before their procedure. VExUS grades were assigned before RHC, blinding ultrasonographers to RHC outcomes. After controlling for age, sex, and common comorbidities, there was a significant positive linear association between RAP and VExUS grade (p < 0.001, R2 = .68). VExUS had a favorable AUC for prediction of a RAP ≥ 12 mmHg (0.99, 95% CI 0.96-1) compared to IVC diameter (0.79, 95% CI 0.65–0.92). These results suggest a strong physiologic correlation between VExUS and RAP in a diverse patient population, and support future studies of VExUS as a tool to assess venous congestion and guide management in a variety of critical illnesses.
Topical Tacrolimus for Vernal Keratoconjunctivitis: A Systematic Review
Authors: Chandra Michael , Angelina Monica 
Published: March 2023

Abstract
Purpose
Vernal keratoconjunctivitis (VKC) is a severe ocular inflammatory disease that requires potent anti-inflammatory drugs as a reliever. Despite the unpopularity, tacrolimus could admirably control inflammations. However, studies regarding topical tacrolimus effectivity for VKC are still lacking.
Methods
We conduct a systematic review to evaluate the effect of topical tacrolimus for VKC. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) checklist for this systematic review. Relevant articles were retrieved from Cochrane Library, PubMed, EBSCO Host, and ProQuest database. We specifically included publication in English. This study was registered with PROSPERO under the number CRD42022302291.
Results
A total of 7 studies were included, involving 232 participants, with a mean age of 10.6 ± 4.3 years. In general, topical tacrolimus reduced the total objective signs score (TOSS) as well as the total subjective symptoms score (TSSS). Adverse events reported from topical tacrolimus were insignificant.
Conclusion
The results of this systematic review suggest that topical tacrolimus could significantly reduce VKC signs and symptoms. Further randomized trials and analyses are required to provide more evidence regarding this intervention.
Selenium protects yellow catfish from low temperature damage via the perspective analysis of metabonomics and intestinal microbes
Authors: Hu Junru , Wang Lei , Zhao Hongxia , Lu Huijie , Peng Kai , Huang Wen , Liu Zhenxing , Liu Ding , Sun Yuping 
Published: March 2023

Abstract
Selenium is an essential trace element in animals, which can improve growth performance and anti-stress ability. Temperature is a crucial environmental factor affecting the health of fish, while Pelteobagrus fulvidraco (P. fulvidraco) can not tolerate low temperature. Does dietary selenium affect the resistance to low temperature stress of this fish? Metabolomics and microbiome analysis were used to investigate it. Feeding results showed selenium supplementation increased the weight gain rate (WGR), specific growth rate (SGR), and survival rate (SR) of P. fulvidraco, and decreased the feed conversion rate (FCR) (P > 0.05), under low temperature stress, selenium also enhanced the glutathione peroxidase (GSH-Px) activity, reduced the malondialdehyde (MDA) content in serum, but these differences were not significant (P > 0.05). Metabonomics analysis revealed lipids and lipid-like molles, organic acids and derivatives, fatty acyls were the mainly differential metabolites caused by selenium, among these, indoles and derivatives, steroids and steroid derivatives, glycerolipids and sphingolipids were significantly up-regulated, while erythrina alkaloids, organic oxygen compounds, organic nitrogen compounds and purine nucleosides were significantly down-regulated. Choline, linoleic acid and glycerophospholipid metabolism pathways, d-arginine and d-ornithine metabolism, valine, leucine, and isoleucine degradation and biosynthes pathways as well as pyrimidine metabolism pathways produced these metabolites. In addition, selenium supplementation also increased the diversity of intestinal microbes in P. fulvidraco and decreased the relative abundance of Plesiomonas under low temperature stress, however, the intestinal microbes change did not affect the metabolites production. In summary, selenium supplementation actived lipid, carbohydrate and amino acid metabolism for energy substances provision, meanwhile reduced oxidation and other harmful substances production, increased the intestinal microbes diversity of P. fulvidraco to improve the low temperature stress resistance.
Fabrication of supramolecular system derived from Poly β-cyclodextrin coupling quinoline derivative and its fluorescence sensing of zinc ion in pure water environment
Authors: Zhu Wenping , Chen Yinlong , Liu Zengchen , Yang Weijie , Li Yanxia , Chen Yahong , Li Qingfeng , Wang Baodui 
Published: March 2023

Abstract
Cyclodextrin (CD) is an important guest material owing to the water solubility and biocompatibility. In the paper, an organic small molecule was synthesized. According to supramolecular self-assembly, the organic molecule was bounded to the cavity of Poly β-cyclodextrin, which was characterized by IR, SEM and TEM et. al. After self-assembly interaction, the morphology has changed obviously comparing with precursors. Simultaneously, the supramolecular self-assembly complex exhibited good water solubility. Moreover, By Gaussian calculation, the high binding activity between organic molecule and cyclodextrin was confirmed. By fluorescence investigation, the supramolecular system showed high fluorescence sensing activity for Zn2+ in pure water environment, which could track the dynamic change of Zn2+ in organisms. In addition, the supramolecular system exhibited low cytotoxicity. The work provided an interesting pathway for constructing water-soluble and hypotoxic fluorescence sensor for Zn2+.
Effect of Gamma-Irradiated Borosilicate Glass on Ciprofloxacin Drug Delivery and Cytotoxicity: In-vitro Study for Medical Applications
Authors: Tiama Taha M. , gweily Noha , Ibrahim Medhat A. , Elshaer Yasser H. 
Published: March 2023

Abstract
Modified borosilicate glasses (BSG) with the composition [50 B2O3-5 SiO2-15 ZnO-30 CaO] were fabricated with melt quenching technique. Samples were then irradiated with gamma radiation at dose rate of 1.4 kGy/h at a temperature of 30°C. Samples were characterized with Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX). FTIR and SEM monitored the microstructural changes of BSG samples before and after gamma irradiation with doses of 50, 100 and 200 M rad, with 50 M rad step. Furthermore, to verify the transformation of the glass and the presence of bioactive glass particles in BSG samples, EDX was implemented to detect oxygen peaks, which are the main component of BSG. Later on, the efficacy of gamma-irradiated BSG in Ciprofloxacin drug delivery and cytotoxicity were studied to be dedicated for medical applications.
A taste for local food: Analysis of archaeological cereal-based foods from the East of England
Authors: Carretero Lara González 
Published: March 2023

AbstractThe study of food preparation and cooking is a research area which is rapidly evolving within archaeobotany and archaeological sciences. Many are the methods available today for the investigation into past culinary and dietary choices and, within these, high-resolution microscopy of preserved remains of cooked foods has become a leading approach. This research presents the application of a fully integrated microscopic approach to the study of archaeological remains of food recovered from sites in Cambridgeshire, England. Applied methods included optical, digital and scanning electron microscopy for the detailed analysis of the identified food products. For the first time, this paper presents the results from the analysis of charred food remains recovered from sites in Britain, specifically from the A14 Cambridge to Huntingdon Improvement Scheme excavations led by MOLA Headland Infrastructure.
Treatment paradigm and prognostic factor analysis of rectal squamous cell carcinoma- a retrospective study
Authors: Liu Rui , Zhang Jiahui 
Published: March 2023

Abstract
Background：Rectal squamous cell carcinoma（rSCC）is a rare pathological type of rectal malignant tumors. There is no consensus on the treatment paradigm of patients with rSCC. This study aims to provide a paradigm for clinical treatment via analyzing the efficacy of different treatment regimens for patients with different TNM stages.
Methods：Patients diagnosed with rSCC between 2010 and 2019 were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. According to the TNM staging system, Kaplan-Meier（K-M）survival analysis was used to identify the survival benefits of patients with rSCC in different treatment groups. The Cox regression method was used to identify independent prognostic risk factors. Nomograms were evaluated by Harrell's concordance index, calibration curves, DCA and K-M curve.
Results：463 patients with rSCC were extracted from the SEER database. Survival analysis showed that there was no significant difference in cancer specific survival (CSS) among radiotherapy（RT）, chemoradiotherapy（CRT）and surgeryin Stage 1 (P = 0.249). In TNM Stage 2, there was significant difference in CSS among surgery, RT, and CRT (P = 0.003). In TNM Stage 3, there was significant difference in CSS between CRT, no treatment and CRT plus surgery (P < 0.001). In TNM Stage 4, there was significant difference in CSS between CRT, no treatment and CT (P = 0.041). COX regression analysis showed that Age, Marital status, N, M, CEA, PIN, Size, RT, CT, and surgery were the independent risk factors. The 1-, 3-, and 5-year C-index was 0.869,0.777,0.759, respectively. The calibration curve showed that the model had excellent calibration. The DCA curve showed that the model had excellent clinical application value.
Conclusion：RT or surgery is recommended for patients with rSCC at Stage 1, and CRT is recommended for patients with rSCCat Stage 2, Stage 3, and Stage 4. Age, marital status, N, M, PIN, size, RT, CT and surgery are independent risk factors for CSS in patients with rSCC. The prediction model composed of the above independent risk factors has excellent prediction efficiency.
Mycorrhizal-based inoculants in the root microbiome enhanced phytocannabinoid production in medical Cannabis cultivars
Authors: Ahmed Bulbul , Beneš František , Hajšlová Jana , Fišarová Lenka , Vosátka Miroslav , Hijri Mohamed 
Published: March 2023

Abstract
Background
The root microbiome of medical Cannabisplants has been largely unexplored due to past legal restrictions in many countries. To gain insight into the microbial communities of Cannabis sativaL. cultivars with different tetrahydrocannabinol (THC) and cannabidiol (CBD) profiles, a greenhouse trial was carried out with and without inoculants added to the growth substrate. Illumina MiSeq amplicon sequencing of the bacterial 16S rDNA and fungal ITS was used to analyze the root and rhizosphere microbiome of the five cultivars.
Results
Plant biomass production showed higher in three of five cultivars with K2 treatments (Rhizophagus irregularis and forest microbial suspension). Blossom dry weight of THE cultivar was greater when inoculated with R. irregularis and microbial suspension than no inoculation. Twelve phytocannabinoid compounds in mature Cannabisvaried among cultivars and were affected by inoculants. For example, CBG concentration was higher in CCL cultivar in response to F treatment than other treatments; and CBGA production was higher in ECC cultivar with K1 treatments. We found microbes which were shared among cultivars, Terrimicrobium sp., Actinoplanes sp., and Trichoderma reeseiwere shared by the cultivars ECC-EUS-THE, CCL-ECC, and EUS-THE, respectively.
Conclusion
This study showed that inoculants influenced the production of phytocannabinoids in five Cannabis cultivars. The microbial diversity and community structure associated with Cannabisroots and rhizosphere may be useful in identifying key taxa for inclusion in Cannabis inoculants.
An assessment of potentially space weather causing CMEs through analysis of associated interplanetary type II solar radio bursts and solar energetic particle events
Authors: Umuhire A. C. , Gopalswamy N. , Uwamahoro J. 
Published: March 2023

Abstract
We report on the results of a study conducted to analyze and characterize the space weather consequences of CMEs as inferred from the associated interplanetary (IP) type II Solar Radio Bursts (SRBs) during part of solar cycle (SC) 24. A sample of 39 metric type II bursts with Decametric-Hectometric (DH) counterpart was analyzed. The properties of SRBs are divided in three groups: all metric type II bursts with DH counterparts (m-DH), m-DH accompanied by a Solar Energetic Particle (SEP) event (m-DH-SEP), and m-DH not accompanied by SEP events (m-DH-NonSEP) were compared and the results are presented. We analysed the CME parameters such as widths, speeds and residual accelerations for the three groups. Among 39 m-DH type II bursts analysed in this study, 21 (54%) are accompanied by SEP events. The average CME speed of m-DH SEP-accompanied events (1198 km/s) is higher than both the m-DH non-SEP events (722 km/s) and all m-DH events (978 km/s). We found that the fraction of halos increases in the order of (i) m-DH-SEP (90.5%), (ii) m-DH (74.3%) and (iii) m-DH-NonSEP (55.6%). The sky-plane speed of CMEs and logarithmic peak intensity of SEP events are highly correlated (Pearson’s correlation coefficient cc = 0.802 with a standard error of SE cc = 0.137). Among 21 m-DH-SEP events, 15 originated from the western longitude and this is consistent with their solar source connected to the observer near Earth, hence a significant space weather implication. In general, the obtained results confirm previous findings that fast and wide (FW) CMEs are effective in accelerating energetic particles and DH type II bursts are good indicators of such events.
Cortical Grey Matter Changes, Behavior and Cognition in Children with Sleep Disordered Breathing
Authors: Tan Brendan , Tamanyan Knarik , Nixon Gillian M , Davey Margot J , Ditchfield Michael , Horne Rosemary SC 
Published: March 2023

Abstract

This paper investigated cortical thickness and volumetric changes in children to better understand the impact of obstructive sleep disordered breathing (SDB) on the neurodevelopment of specific regions of the brain. We also aimed to investigate how these changes were related to the behavioral, cognitive and autonomic deficits observed in the condition. Neuroimaging, behavioral and sleep data were obtained from 30 children (15 non snoring controls, 15 referred for assessment of SDB) aged 7 to 17 years. Gyral-based regions of interest were identified using the Desikan-Killiany Atlas. Student’s T-tests were used to compare regions of interest between the controls and SDB groups. We found cortical thickness was significantly greater in the right caudal anterior cingulate and right cuneus regions and volumetric increases in the left caudal middle frontal, bilateral rostral anterior cingulate, left, right and bilateral caudate brain regions in children with SDB compared to controls. Neither cortical thickness nor volumetric changes were associated with behavioral or cognitive measures. The findings of this study indicate disruptions to neural developmental processes occurring in structural regions of the brain; however, these changes appear unrelated to behavioral or cognitive outcomes.
Dynamics and Entrainment Mechanism of the Jet Flows from an Elliptical Nozzle: Time-Resolved Tomographic PIV Measurements
Authors: Xin Zeng , ZHANG Yuyao , HE Chuangxin , LIU Yingzheng 
Published: March 2023

Abstract
In this study, the coherent structure dynamics and entrainment capability of elliptical jets emitted from an elliptical nozzle with aspect ratio (AR) values of 1 (i.e., a circular jet), 2, and 4 at a fixed Reynolds number of 3,000 were experimentally characterized by flow reconstruction using tomographic particle image velocimetry and modal decomposition using the spectral proper orthogonal decomposition method. Statistical analysis indicated that the elliptical synthetic jet had a greater entrainment rate and momentum flux than the circular jet. The temporal dynamics of the coherent structure showed that all the jets emitted from the elliptical nozzle at AR = 1 (i.e., a circular jet), 2, and 4 had a dominant frequency at Strouhal number (St) = 0.39, representing the leading Kelvin–Helmholtz (K-H) vortex ring in each jet. The frequency of the trailing vortex was also at St = 0.39 at AR = 1 and 2, whereas that at AR= 4 was at St = 0.70. It was found that vortex ring pairing and merging, as well as axis switching, are common in elliptical jets. When AR was 4, there was also a large-scale single vortex ring between the two merged vortex rings, this large-scale single vortex alone underwent axis transformation and breakage. The merger always occurred in the major plane in elliptical jets because in the major plane, the leading and trailing vortices approached each other as a result of self-induction and mutual induction. The entrainment rate was strongly correlated with the K-H vortex ring dynamics in circular jets and weakly correlated with the K–H vortex ring passing in elliptical jets. The entrainment appeared in the upstream part of the K-H vortex ring structures in circular jets, whereas it was enhanced in the axis-switching region in elliptical jets. An analysis of the contribution of each mode to mass entrainment showed that the entrainment rate of the elliptical nozzle was better than that of the circular nozzle, mainly because many streamwise vortices were generated.
Agrobiodiversity in Quilombola Swiddens in the Municipality of Abaetetuba, Amazon Forest of Pará
Authors: Gonçalves Janaina Pinheiro , Torres Marivaldo Gomes , Pereira José Alex Batista , Alves Raynon Joel Monteiro , Miranda Thyago Gonçalves , Tavares-Martins Ana Cláudia Caldeira , Andrade Eloísa Helena de Aguiar 
Published: March 2023

Abstract
This study aimed to characterize and analyze the swiddens and the socioeconomic, cultural and environmental scenario of the Quilombola communities Ramal Bacuri and Rio Baixo Itacuruçá in the municipality of Abaetetuba, Pará. The sampling of informants was non-probabilistic and used the snowball method and the data were collected through participant observation, field journals, and semi-structured interviews with the support of forms for characterization of the swiddens, productive aspects and difficulties in swidden management. The data were analyzed by content analysis. The results indicated that the swiddens are traditionally planted on terra firme (non-flooded) areas (primary and secondary forests, the latter known as “capoeiras”) and that environmental, socioeconomic and cultural conditions are factors that influence the perspective of the product with the highest market value. A relative diversity of annual and perennial species was observed in the two communities, which serve as food and are also used for income generation. The conscious use of “capoeiras”, the control of fires by firebreaks, and the maintenance of varieties of “manivas” are knowledge/practices that favor the conservation of agrobiodiversity. Therefore, the swiddens of Bacuri and Itacuruçá are agrobiodiverse spaces that encompass biological diversity and traditional knowledge associated with the management and conservation of resources.