bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stimulation of human synovial fibroblast plasminogen activator production by mononuclear cell supernatants.
Authors: Hamilton J A , Slywka J 
Published: December 2022

Abstract
Conditioned medium from concanavalin A-stimulated human peripheral blood mononuclear cells (c-MCCM) stimulates the plasminogen activator (PA) production of nonrheumatoid human synovial fibroblasts obtained from explant cultures. The effect of this synovial fibroblast-stimulating activity is observed within 2 to 4 hr and requires RNA and protein synthesis. Reversible morphological changes in the synovial cells can be observed as a result of c-MCCM action. These enzymatic and morphologic changes are similar to some of the effects of transforming viruses and tumor promoters on target cells. The possible significance of these data for an understanding of the cellular interactions involved in the formation and function of the rheumatoid "pannus" is discussed.
Interaction of C-reactive protein with liposomes. III. Membrane requirements for binding.
Authors: Mold C , Rodgers C P , Richards R L , Alving C R , Gewurz H 
Published: December 2022

Abstract
We have previously presented a model for CRP-membrane interactions using liposomes composed of dimyristoyl phosphatidylcholine (DMPC), cholesterol (CHOL), stearylamine (SA), and galactosyl ceramide. We have shown that the interaction of cRP with these liposomes in the presence of human serum results in consumption of hemolytic C and membrane lysis. In the present paper we have directly examined the binding reaction between CRP and liposomes using radiolabeled CRP. We have found that this binding is more characteristic of CRP interactions with polycations than CRP interactions with phosphocholine- (PC) containing molecules. CRP binding to liposomes was dependent on the presence of SA in the membrane and could occur with dimyristoyl phosphatidylethanolamine in place of DMPC. The binding was not inhibited by ethylenediaminetetraacetate (EDTA) but could be inhibited by CaCl2, whereas CRP binding to PC-Sepharose was inhibited by EDTA and required CaCl2. We have further examined the effects of changes in membrane composition on CRP binding to liposomes. In liposomes with a limiting density of SA, we found increased CRP binding with changes in composition that would increase membrane fluidity. In most cases, the amount of CRP binding correlated with the amount of C activation observed previously. However, increasing the amount of CHOL in the membrane was found to increase C activation while decreasing CRP binding. These findings indicate that CRP binding to membranes and subsequent C activation can occur through cationic molecules as well as phospholipids.
Relative lack of Epstein Barr virus (EBV) receptors on B cells from persistently EBV seronegative adults.
Authors: Gervais F , Wills A , Leyritz M , Lebrun A , Joncas J H 
Published: December 2022

Abstract
Viral receptors are essential for the entry of the virus into the cell. EBV receptors can be detected on fresh lymphocytes by a technique that uses EBV-coupled tanned red blood cells that form rosettes with lymphocyte-bearing receptors. This technique was found to detect viral receptors only and not surface immunoglobulins. T cell depletion of the lymphocyte population showed that these receptors were present on B lymphocytes. Study of the presence of these EBV receptors on the surface of fresh lymphocytes from 66 subjects (age 2 to 66), selected out of a group of over 2000 individuals, showed that the majority of these donors had receptors for the virus. However, a few of these adults persistently failed to develop anti-EBV antibodies, even if they were in close contact with the infectious agent. The lymphocytes of 11 such individuals were found to be lacking EBV receptors. Transformation assay of these lymphocytes did not give rise to lymphoblastoid cell lines whereas lymphocytes from 4 individuals, who were EBV seropositive or seronegative but receptor positive, yielded permanent lymphoblastoid cell lines. This would suggest that a few EBV seronegative adults (less than 0.5%) display natural resistance to EBV transformation of their lymphoid cells as a result of absolute or relative lack of EBV receptors on these cells.
Mycoplasma-mediated activation of normal mouse lymphocytes: induction of cytotoxic lymphocytes and lymphocyte transformation by Mycoplasma arthritidis are under Ir gene control.
Authors: Cole B C , Daynes R A , Ward J R 
Published: December 2022

Abstract
The ability of M. arthritidis, a potent arthritogenic agent, to induce normal unsensitized lymphocytes to undergo blast transformation and to become nonspecifically cytotoxic for syngeneic and allogeneic target fibroblasts was found to be dependent upon the donor strain of mouse. Lymphocytes from CBA (H2k) and BALB/c (H2d) mice were identified as high responders and lymphocytes from C56BL/10 mice (H2b) as low or nonresponders. In the cytotoxicity assay the extent of cell lysis was dependent upon the origin of both the effector cell and the target cell. By comparing the ability of M. arthritidis to activate lymphocytes from various congenic and H-2 recombinant mouse strains we demonstrated that control of the degree of responsiveness was determined by the H2 gene complex. Further studies revealed that the region controlling induction of cytotoxic lymphocytes is located to the right of the I-J subregion and to the left of the D region, most probably the I-E and/or I-C subregions of the major histocompatibility complex. These observations may be of importance in regard to the susceptibility of different mouse strains to the pathogenic effects of M. arthritidis.
Correlation between clinical activity of systemic lupus erythematosus and the amounts of DNA in DNA/anti-DNA antibody immune complexes.
Authors: Morimoto C , Sano H , Abe T , Homma M , Steinberg A D 
Published: December 2022

Abstract
The relationship between clinical activity of systemic lupus erythematosus (SLE) and molecular sizes of DNA fragments isolated from DNA/anti-DNA antibody immune complexes were examined. Among sera from twenty-eight patients with SLE examined, three different molecular sizes of DNA were identified, namely, DNA of m.v. 25,000 with 30 to 50 base pairs (bp); m.w. 100,000 with 150 to 200 bp; and m.w. 200,000 with 300 bp. On the basis of the molecular sizes of DNA fragments, we can divide patients with SLE into four groups. The first group contained DNA predominantly of m.w. 25,000. The second group contained DNA predominantly of m.w. 100,000. The third group contained DNA of m.w. 200,000. The fourth group contained both m.w. 25,000 and 100,000. A method to quantitate the amounts of DNA in DNA/anti-DNA immune complexes was developed. The amount of DNA fragments was estimated by measuring the amount of 32P-phosphate incorporated into 5 ends of DNA. Patients with severe disease tended to have greater amounts of DNA (up to 400 ng/ml serum). Two serial studies also support this result. Thus, the quantitative analysis shows that the amount of DNA in the immune complexes is highly correlated with disease activity (r = 0.864; p less than 0.001). These results suggest that DNA/anti-DNA immune complexes may play some role in the pathogenesis of lupus nephritis.
Oligoclonal IgG bands with and without measles antibody activity in sera of patients with subacute sclerosing panencephalitis (SSPE).
Authors: Mehta P D , Patrick B A , Thormar H , Wisniewski H M 
Published: December 2022

Abstract
Oligoclonal IgG bands from SSPE sera were isolated by combination of Protein A-Sepharose 4B column and preparative isoelectric focusing gel procedures. Each eluted fraction, when examined in analytic IEF, showed two or three individual bands with isoelectric points close to one another, compared to approximately fifteen IgG bands seen in whole serum. When the bands were tested for measles antibody activity in immunofixation with measles virus followed by peroxidase staining, the bands eluted in pH region 8.5 to 9.3 were found to be measles specific, whereas those in pH 7.0 to 8.4 lacked significant measles activity. When eluted fractions containing groups of bands were absorbed with measles virus, the bands in pH region 8.5 to 9.3 were removed, whereas those in pH 7.0 to 8.4 region remained unchanged; this indicated that a number of oligoclonal IgG bands without measles virus activities are present in SSPE. The bands lacking measles-specific activity may be synthesized against other infectious agents or they may represent nonspecific activation of B cell clones.
Anti-idiotypes to monoclonal anti-H-2 antibodies. III. Syngeneic anti-idiotypes on antibodies induced by in vivo administration of xenogeneic anti-idiotypes.
Authors: Bluestone J A , Auchincloss H , Cazenave P A , Ozato K , Sachs D H 
Published: December 2022

Abstract
Idiotype-positive molecules induced by xenogeneic anti-idiotypes (anti-Id) to monoclonal anti-H-2 antibodies were studied using syngeneic anti-Id reagents. Syngeneic reagents detected idiotypes on both H-2 antigen-binding and nonbinding Id' induced by xenogeneic anti-Id. In addition, idiotopes were readily detected by alloimmunization after xenogeneic anti-Id reagents interact with some of the same antibodies as syngeneic anti-Id and support the hypothesis that treatment with xenogeneic anti-Id may affect a physiologically important idiotypic network.
Virulent P. berghei malaria: prolonged survival and decreased cerebral pathology in cell-dependent nude mice.
Authors: Finley R W , Mackey L J , Lambert P H 
Published: December 2022

Abstract
The course of lethal Plasmodium berghei infection was examined in nu/+ and T cell-deficient nu/nu BALB/c mice. A rapidly fatal neurologic syndrome, including ataxia, hemiparesis, and seizures, was seen in the nu/+ mice early in the infection, whereas this syndrome was absent in the nu/nu mice. The nu/nu mice also developed anemia more slowly, had lower levels of immune complexes and total IgG, and had smaller decreases in serum C3 compared with the nu/+ mice. Histopathologic examination of the brains revealed cerebral malaria lesions, including vascular plugging and micro-hemorrhages, in the nu/+ mice but not in the nu/nu mice. Cerebral lesions similar in frequency and severity to those in nu/+ mice developed in nu/nu mice given spleen cells from normal nu/+ mice. The results suggest that an intact immune system is necessary for the expression of cerebral malaria.
Purification and characterization of a high molecular weight eosinophil chemotactic factor from Schistosoma japonicum eggs.
Authors: Owhashi M , Ishii A 
Published: December 2022

Abstract
A high m.w. eosinophil chemotactic factor (ECF-SjE) was isolated and purified from a soluble egg antigen preparation (SEA) of Schistosoma japonicum by gel filtration on Sephacryl S-200, anion-exchange chromatography on DE52, and isoelectric focusing. ECF-SjE had a m.w. of more than 900,000 and an isoelectric point of 4.1. It contained 40% (w/w) sugar residues and bound to concanavalin A (Con A). The chemotactic activity of ECF-SjE was heat stable (100 degrees C, 60 min) and resistant to pronase digestion, but was destroyed by periodate oxidation. IgG antibody to ECF-SjE was detected in the serum of a rabbit infected with S. japonicum, demonstrating the antigenic nature of ECF-SjE. The antigenicity of ECF-SjE was also sensitive to periodate oxidation. Thus, ECF-SjE is a glycoprotein or proteoglycan from the eggs of S. japonicum, and the sugar chain is important for the expression of chemotactic and antigenic activities. However ECF-SjE differs from the major allergenic components of S. japonicum (JEAL) in m.w. and isoelectric point. A low m.w. eosinophil chemotactic factor was also detected in SEA. Together they are proposed to have a role in the direct accumulation of eosinophils in the egg-induced granulomas in S. japonicum infection.
Monoclonal antibodies to human alpha-interferon and their use for affinity chromatography.
Authors: Novick D , Eshhar Z , Rubinstein M 
Published: December 2022

Abstract
A new screening procedure has been developed and used for the identification of three hybridomas secreting monoclonal antibodies to human leukocyte interferon (IFN-alpha). The screening procedure is based on immune precipitation with a second antibody, acid dissociation of the precipitate, and bioassay of the recovered interferon. This procedure selects for monoclonal antibodies that are suitable for affinity chromatography, as demonstrated by construction of immunoadsorbents from these monoclonal antibodies and purification to homogeneity in IFN-alpha.
Results from the Use of the Blood Clot Method of Immunization
Authors: Anderson John F. , Leonard George F. 
Published: December 2022

Abstract
Zinsser and Grinnell in 19252 reported a novel method for the immunization of horses against pneumococcus, and suggested its application in the case of other bacteria. When this procedure came to our attention, we were immediately interested in the possibility of its use, particularly in the immunization of horses against certain types of streptococci, pneumococci and other bacteria. We have used the method for the immunization of horses against different strains of streptococci, pneumococci, the green producing diplococcus isolated by Tunnicliff from cases of measles, and a combination of B. coli and streptococci.
The essential feature in the blood clot method is the injection of whole oxalated blood to which the bacteria have been added, either immediately before injection or sometime previous, together with sufficient calcium chloride to induce the formation of a clot within a few minutes. It was the idea of the originators that the toxic factor from the pneumococcus developed when the pneumococci grew in the blood under conditions analogous to those prevailing in pneumonia, although they were undecided whether this toxic factor was antigenic.
Interferon-gamma induces class I HLA and beta 2-microglobulin expression by human amnion cells.
Authors: Hunt J S , Wood G W 
Published: December 2022

Abstract
In contrast to other human nucleated cells, amnionic epithelial cells do not normally express class I HLA antigens. It is not known whether the failure of display of those antigens occurs because genes for HLA expression are deleted or are defective, or if gene expression is repressed. The results of the present study demonstrate that amnionic epithelial cells exposed to IFN-gamma express levels of class I HLA antigens and beta 2-microglobulin that are readily detectable in immunoperoxidase and immunofluorescence stains. The findings suggest that failure of transcription may be responsible for failure of normal expression of class I HLA antigens and beta 2-microglobulin by cells of the amnion membrane. Class II antigens, which are normally expressed by very few cell types, were not expressed by amnion cells under any test conditions.
Reorientation and fusion of cytotoxic T lymphocyte granules after interaction with target cells as determined by high resolution cinemicrography.
Authors: Yannelli J R , Sullivan J A , Mandell G L , Engelhard V H 
Published: December 2022

Abstract
The interaction of murine cytotoxic T lymphocyte (CTL) clones with human lymphoblastoid target cells was studied in thin preparations by using high resolution cinemicrography. CTL not bound to target cells were morphologically polar, possessing a broad leading edge containing the nucleus, and a tapered tail containing a large number of granules. The CTL were observed to move by the extension of pseudopods from the leading edge. Initial contact with a target cell was made via the leading edge of the CTL. If the human target cell expressed the appropriate HLA antigen, distinct morphologic changes occurred in the CTL as early as 2 min after initial contact. The CTL rounded up, and the nucleus moved from a position adjacent to the zone of contact to be replaced by the cytoplasmic granules. Redistribution of the granules was completed as early as 10 min after initial contact was made. These morphologic changes did not occur when the CTL made contact with other CTL, or with target cells that did not express the appropriate HLA antigens. In studies that make use of Nomarski optics, an apparent fusion of CTL cytoplasmic granules with the membrane in the vicinity of the target cell contact area was observed 4 min after binding, and before granule reorientation was complete. These data provide direct evidence for the occurrence of both reorientation of the cytoplasmic contents and granule fusion in CTL with a time course similar to that of administration of the lethal hit.
Clonal analysis of the anti-Qa-1 cytotoxic T lymphocyte repertoire: definition of the Qa-1d and Qa-1c alloantigens and cross-reactivity with H-2.
Authors: Aldrich C J , Jenkins R N , Rich R R 
Published: December 2022

Abstract
To characterize the four common Qa-1 allelic products, we examined in detail the CTL-defined determinants encoded by Qa-1. In previous studies with anti-Qa-1 CTL and alloantisera, investigators have described antigenic determinants present on Qa-1a and Qa-1b antigens, but they have defined Qa-1c and Qa-1d exclusively by their cross-reactivity with Qa-1a and/or Qa-1b determinants. To delineate further the CTL-defined determinants encoded by Qa-1d, we generated CTL clones with Qa-1d specificity and demonstrated that the Qa-1d molecule expressed determinants that were not detected on Qa-1a, Qa-1b, or Qa-1c target cells. Other CTL clones derived from anti-Qa-1d MLC recognized new antigenic determinants on Qa-1c that cross-reacted with Qa-1d. Each of the four common Qa-1 phenotypes was shown to exhibit unique antigenic determinants. In addition, Qa-1d anti-Qa-1a and Qa-1d anti-Qa-1b CTL confirmed extensive cross-reactivity among these Qa-1 alloantigens. Analysis of CTL from these four immunizations also resulted in the isolation of Qa-1a-specific and Qa-1d-specific CTL clones that cross-reacted with H-2Df and H-2Ks, respectively.
Ia-positive nonlymphoid cells and T cell development in murine fetal thymus organ cultures: monoclonal anti-Ia antibodies inhibit the development of T cells.
Authors: DeLuca D 
Published: December 2022

Abstract
A fetal thymus organ culture system has been developed to study the differentiation of murine thymus-derived immunocompetent cells (T cells) such that cell yields can be easily monitored. This system has been used to study the effects of monoclonal anti-I-A antibodies on the growth of T cells. The addition of anti-I-A antibodies, but not anti-H2K monoclonal antibodies, to fetal thymus organ cultures resulted in a decreased yield of lymphoid cells. Anti-I-A-treated cultures did not produce cells that gave an immune response in MLC assays. Anti-I-A antibodies stained a small subpopulation of nonlymphoid cells in untreated cultures by indirect immunofluorescence that were no longer detectable in cultures that had been pretreated with anti-I-A antibody. Culture of fetal thymus lobes at low temperature (20 degrees C) for 1 wk resulted in a decrease in lymphocyte production, as well as a concomitant increase in the frequency of Ia-positive nonlymphoid cells. Co-culture of fetal liver or anti-thy-1 plus complement-treated adult bone marrow with such Ia-positive cell-enriched fetal thymus lobes at 37 degrees C resulted in the production of T cells. Anti-Thy-1.1 or -1.2 staining by indirect immunofluorescence of cells obtained from co-cultures that differed at the Thy-1 locus showed that the T cells produced were derived from the bone marrow or fetal liver. T cell production occurred in both syngeneic and allogeneic cocultures. However, if co-cultures were made by using 14-day gestation fetal thymus instead of fetal liver or bone marrow as donors of T cell precursors, T cell growth was observed only in syngeneic combinations. These results suggest that Ia-positive nonlymphoid cells play a role in the development of T cells in the fetal thymus, and that "thymus processed" T cell progenitors (but not the more immature progenitors in the fetal liver or bone marrow) are self-Ia restricted in their differentiation.
Two major classes of mitogen-reactive B lymphocytes defined by life span.
Authors: Freitas A A , Rocha B , Coutinho A A 
Published: December 2022

Abstract
The relative numbers of short- and long-lived mitogen-reactive B cells in the peripheral pool were evaluated by studying the decay of lipopolysaccharide (LPS)-reactive B lymphocytes in LPS nonresponder, histocompatible hosts for periods of up to 3 wk after cell transfer. The results obtained demonstrate the existence of two major classes of mitogen-reactive B cells defined by life span. The "short-lived" cell component comprises about 80 to 90% of the reactive cells and decays with a life expectancy of 18 to 24 hr. The long-lived cell component, with life expectancies of 10 to 20 days, comprises about 10 to 20% of the reactive cells and is preferentially enriched in circulating B cells. The present ratio for short- and long-lived B cells implies a highly dynamic state for the immune system which must be advantageous in the selection of available repertoires.
Immunoglobulin light and heavy chain isotypes in skin diseases: restricted distribution in bullous pemphigoid and linear IgA bullous dermatosis.
Authors: Flotte T J , Baird L G 
Published: December 2022

Abstract
To assess the heterogeneity of immunoglobulins involved in various skin diseases, direct and indirect immunofluorescence studies of skin biopsies and sera, respectively, for kappa and lambda light chains, were performed. The anti-basement membrane zone (anti-BMZ) antibodies of patients with bullous pemphigoid showed a predominance of kappa light chains, and patients with linear IgA bullous dermatosis showed a predominance of one light chain that was sometimes kappa and sometimes lambda. The bullous pemphigoid autoantibodies were then studied for IgG subclass distribution; a predominance of IgG4 was found. Although other explanations are possible, the light chain restriction in bullous pemphigoid most likely reflects heavy chain restriction and preferential association of heavy and light chain isotypes. The basis of the heavy chain restriction is not apparent. The light chain restriction in linear IgA bullous dermatosis may represent a restricted idiotypic repertoire.
Human alveolar macrophages: HLA-DR-positive macrophages that are poor stimulators of a primary mixed leukocyte reaction.
Authors: Lipscomb M F , Lyons C R , Nunez G , Ball E J , Stastny P , Vial W , Lem V , Weissler J , Miller L M 
Published: December 2022

Abstract
Previous studies demonstrated that alveolar macrophages (AM) from most normal human volunteers failed to stimulate the antigen-induced proliferation of peripheral blood T lymphocytes although greater than 90% of AM expressed HLA-DR antigens. The current studies establish that AM also fail to induce allogeneic peripheral blood mononuclear cells to proliferate in a mixed leukocyte reaction (MLR). Suppressive activity by AM was not an explanation for their failure to induce an MLR. Indirect immunofluorescence established the presence of both HLA-DR and DQ antigens on the majority of AM and the persistence of these antigens on cells in culture for up to 6 days, the period of time required to observe a maximal MLR. Metabolic labeling experiments also demonstrated that HLA-DR antigens were synthesized by AM. It was recently reported that AM secrete relatively small amounts of IL 1, an important ancillary signal provided by accessory cells to enhance the stimulation of lymphocyte proliferation. However, addition of optimal concentrations of IL 1 to cultures containing AM failed to enhance the MLR. Thus, there is at least one additional, but as yet undefined, requirement for an accessory cell to induce an optimal MLR besides the display of HLA-D region antigens and the secretion of IL 1. In contrast, AM were effective in specifically stimulating proliferation of alloreactive T cell lines, suggesting that at least some cell lines do not require this nonspecific undefined second signal. We speculate that although AM may not initiate primary immune responses in the lung, they may be important in maintaining immune-mediated inflammatory responses by specifically restimulating already activated T cells.