bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypertension as a prominent manifestation in pediatric Behcet's disease
Authors: Wang Xinning , Zhou Zhixuan , Li Jianguo , Su Gaixiu , Li Xiaohui 
Published: August 2023

Abstract
Objective:
Hypertension caused by vascular Behcet’s disease(BD) is an important prognostic factor of pediatric BD. However, much less is known about it’s clinical features. The objective of this study is to investigate the clinical characteristics of pediatric BD complicated by hypertension.
Methods:
A retrospective study was carried out in pediatric BD complicated by hypertension hospitalized at the Children’s Hospital Capital Institute of Pediatrics from Jan 2009 to Dec 2022.
Results:
Of 65 BD patients, 6 (9.2%) were complicated by hypertension, 4 patients were female, and the median ages of onset and diagnosis were9.8 years and 11.3 years, respectively. Five of the 6 patients with hypertension had right renal artery involvement,and all of them were treated with glucocorticoids and immunosuppressants. Four patients were treated with biological agents. One patient with severe renal artery stenosis received vascular interventional therapy, but it failed. After 3-6 years of follow-up, five patients were found to have renal atrophy, and one patient was in stable condition.
Conclusion:
Hypertension in pediatric BD is mainly caused by renal artery involvement. Early recognition and treatment of vascular involvement in BD is important to prevent poor prognosis.
Odourless vegetable oils as insect pest repellents for short-term protection of various food packaging materials
Authors: Shah Jawad Ali , Vendl Tomas , Aulicky Radek , Stejskal Vaclav 
Published: August 2023

Abstract
The repellent activity of two odourless vegetable oils (sweet almond - SAO and soybean - SO) applied on filter paper and four types of food packaging materials (polyethylene film, plastic gunnysacks, cardboard, and wood) was tested against three species of stored product pests (Sitophilus granarius, Tribolium castaneum and Oryzaephilus surinamensis). The repellence of three doses was evaluated after 24 hours, 7, 14, 21, and 28 days. Both oils exhibited a repellent effect when applied to filter paper, but its effect decreased with time and concentration. The most susceptible species was T. castaneum. The maximum repellence was 84% and 76% for SO and SAO, respectively. On packaging materials, the repellent effect diminished after a certain period of time, ranging from 7 to 28 days. Subsequently, the oils even became attractive. Generally, the repellent effects of both SO and SAO were higher on cardboard than on polyethylene film and plastic gunnysacks. For instance, in the case of SO, the repellence against T. castaneum was 88% 24 hours after application on cardboard, whilst 68% on plastic bag. This work demonstrated the repellent potential of vegetable oils in the short term for the protection of packaged food against stored product pests.
Comparative outcome of different treatment options for acetabulum fractures in elderly individuals - a retrospective analysis of 207 patients.
Authors: Husi Sebastian , Vach Werner , Cadosch Dieter , Jakob Marcel , Saxer Franziska , Eckardt Henrik 
Published: August 2023

Abstract
Purpose: Acetabular fractures are challenging injuries in the heterogeneous population of elderly patients. In patients able to partially bear weight, open reduction and internal fixation (ORIF) is indicated. In frail patients, ORIF combined with primary total hip arthroplasty allows early weight-bearing to preserve independence. This article systematically analyses a treatment algorithm that separates fractures into stable fractures treated conservatively and fractures needing surgical stabilization with osteosynthesis or osteosynthesis plus arthroplasty, dependent on patient characteristics but less on the fracture classification or energetic impact of the trauma.
Methods: Data on patients ≥50 years of age treated for acetabular fractures (2009-2019) were retrospectively analyzed. The primary outcome was loss of independence. In-hospital complications, length of stay, re-operations, the need for walking aids and pain were analyzed as secondary outcomes.
Results: Out of 207 patients, 135 were male, average age was 70 years. Eighty-five patients were treated conservatively, 89 ORIF, and 33 ORIF plus arthroplasty in one operation. The initial morbidity of patients treated with the combined operation was higher than osteosynthesis alone, but the long-term outcome was favorable with less pain and fewer secondary interventions. Age and female gender were associated with the decision to treat the fracture with the combination of osteosynthesis and arthroplasty.
Conclusions: The results suggest that aged and potentially frail patients with acetabular fracture have better long-term outcome after ORIF combined with arthroplasty at the price of an initially higher risk of adverse outcomes. Females were more frequently treated with the combined operation independent of other risk factors
Comparative transcriptome profiling of contrasting finger millet (Eleusine coracana (L.) Gaertn) genotypes under heat stress
Authors: Goyal Etika , Singh Amit Kumar , Mahajan Mahesh Mohanrao , Kumar Kanika 
Published: August 2023

Abstract
Background Eleusine coracana (L.) Gaertn is a crucial C4 species renowned for its stress robustness and nutritional significance. Because of its adaptability traits, finger millet (ragi) is a storehouse of critical genomic resources for crop improvement. However, more knowledge about this crop's molecular responses to heat stress must be gained.Methods and Results In the present study, a comparative RNA sequencing analysis was done in the leaf tissue of the finger millet between the heat-sensitive (KJNS-46) and heat-tolerant (PES-110) cultivars of Ragi in response to high temperatures. On average, each sample generated about 24 million reads. Interestingly, a comparison of transcriptomic profiling identified 684 transcripts which were significantly differentially expressed genes (DEGs) examined between the heat-stressed samples of both genotypes. The heat-induced change in the transcriptome was confirmed by qRT-PCR using a set of randomly selected genes. Pathway analysis and functional annotation analysis revealed the activation of various genes involved in response to stress, precisely heat, oxidation-reduction process, water deprivation, heat shock protein (HSP) and transcription factors, calcium, and kinase signaling. The basal regulatory genes, such as bZIP, were involved in response to heat stress, indicating that heat stress activates genes related to basal regulatory processes or housekeeping. A substantial percentage of the DEGs belonged to proteins of unknown functions (PUFs), i.e., uncharacterized.Conclusion The finding highlights the importance of HSPs, candidate genes, and pathways that can confer tolerance towards heat stress in ragi. These results will provide valuable information to improve heat tolerance in heat-susceptible agronomically important varieties of ragi and other crop plants.
Efficacy of adding sodium hyaluronate eye drops to tobramycin and dexamethasone in the treatment of post- cataract surgery dry eye disease: A Retrospective Analysis and Prospective Questionnaire Assessment
Authors: Amer Ahmed Ali , Ali Essam Ghanim Abu El Wafa , Hamed Mohamed Ateto , elkareem Ashraf Mohammed Gad , Sengab Alaa Abdel Sadek , Awny Islam 
Published: August 2023

Abstract
Background
One of the cataract-related complications is dry eye disease (DED). Dry eye disease imposes a negative impact on the quality of vision (QOV) and quality of life (QOL). This study aimed to compare the efficacy of using a combination of antibiotic and corticosteroid eye drops to using the same combination in addition to sodium hyaluronate eye drops.
Patients and methods:
This study included patients who underwent phacoemulsification for age-related cataract and presented to the ophthalmology department or outpatient clinic of the two hospitals in the study with DED during the period from January 2020 to January 2023. The study involved a two-pronged approach in which we retrospectively analyzed the prospectively maintained data of the patients and prospectively called the patients for an evaluation of the dry eye impact on vision-related function. The study patients were recruited from two hospitals. During the study period, patients who were treated with a combination of antibiotic and corticosteroid eye drops were enrolled in Group A, and those who were treated with the same combination with sodium hyaluronate eye drops were enrolled in Group B.
Results
The present study included 143 patients with post-phacoemulsification DED. Group A included 70 patients, and Group B included 73 patients. There was statistically significant improvement 4 weeks after treatment (p < 0.05) in the two groups. Comparing the two groups showed statistically significant improvements in group B compared to group A (p < 0.05). Assessment of the overall efficacy outcome revealed a statistically significant high percentage of cure (35.6% vs. 1.4%) and high effectiveness (42.5% vs. 13.6%) and a low percentage of effectiveness (21.9% vs. 74.3%) and ineffectiveness (0.0% vs. 5.7%) in Group B compared to Group A (p < 0.001).
Conclusion
Combining sodium hyaluronate eye drops with tobramycin and dexamethasone eye drops yielded obviously better efficacy outcomes compared to using tobramycin and dexamethasone eye drops alone.
Application of Optical Genome Mapping in Complex Chromosomal Structural Rearrangements
Authors: Li Tao , Liu Shanquan , Lai Yi 
Published: August 2023

Abstract
Objective: The aim of this study was to assess the applicability of optical genome mapping in chromosomal karyotype analysis. Optical genome mapping validation was conducted on three patients with complex chromosomal structural rearrangements previously identified through chromosomal G-banding karyotype analysis. The primary objective was to elucidate the breakpoints and patterns of chromosomal recombination.
Methods: A comprehensive analysis and validation were conducted on three patients utilizing various techniques, including chromosome G-banding karyotyping, Chromosome Microarray Analysis, and optical genome mapping.
Results: Combined with the results of optical genome mapping and G-banding karyotyping, all three patients exhibited complex chromosomal rearrangements, with the following karyotype descriptions:
Patient 1: 46,XX,der(9)ins(9)(q34.3q21.1q21.1)inv(9)(q21.1q21.3);
Patient 2: 46,XY,inv(2)(p23q33),t(3;7)(q26.1;q35)inv(7)(q31.2q35);
Patient 3: 46,XY,t(1;4;11)(p21.3;q23;q25)inv(11)(p12q14.3)inv(11)(q14.3 q25).
Conclusion: Optical genome mapping (OGM) demonstrated superior resolution and sensitivity compared to traditional methods like G-banding karyotyping. OGM proved valuable for validating the results obtained from karyotyping analysis, enhancing diagnostic accuracy.
Tuberculum meningioma with recovery of glaucomatous visual field defects after chiasmal decompression: a case report
Authors: Hanai Kaori , Hashimoto Masato , Nakamura Hirohiko 
Published: August 2023

Abstract
Background: To report a case of tuberculum meningioma with recovery of glaucomatous visual field defects after chiasmal decompression.
Case presentation: A 39-year-old woman presenting with headache was found to have a suprasellar tumor compressing the anterior chiasm upwards on magnetic resonance imaging of the brain. The patient had bilateral arcuate retinal nerve fiber layer (RNFL) thinning on optical coherence tomography (OCT). The visual fields showed arcuate scotomas compatible with RNFL loss, suggesting glaucomatous change. But after resection of the mass, which was pathologically diagnosed as meningothelial meningioma, the glaucomatous visual field defects resolved despite the RNFL thinning on the OCT showing no improvement.
Conclusions: Chiasmal compression in this case is extremely rare in the way that it produced arcuate scotoma such as glaucoma rather than temporal visual field loss. There is a possibility that the development of chiasmal compression somehow converted preperimetric glaucoma into a more advanced form accompanied by visual field defects and that the glaucoma reverted to the preperimetric state after chiasmal decompression.
NAT10-mediated RNA ac4C acetylation contributes to the myocardial infarction induced cardiac fibrosis
Authors: Li Jun , Yushanjiang Feierkaiti , Fang Zhao , Liu Wan-li 
Published: August 2023

Abstract
Cardiac fibrosis is featured cardiac fibroblast activation and extracellular matrix accumulation. Ac4C acetylation is an important epigenetic regulation of RNAs that has been recently discovered, and it is solely carried out by NAT10, the exclusive enzyme used for the modification. However, the potential regulatory mechanisms of ac4C acetylation in myocardial fibrosis following myocardial infarction remain poorly understood. In our study, we activated fibroblasts in vitro using TGF-β1 (20 ng/mL), followed by establishing a myocardial infarction mouse model to evaluate the impact of NAT10 on collagen synthesis and cardiac fibroblast proliferation. We utilized a NAT10 inhibitor, Remodelin, to attenuate the acetylation capacity of NAT10. In the cardiac fibrosis tissues of chronic myocardial infarction mice and cultured cardiac fibroblasts in response to TGF-β1 treatment, there was an elevation in the levels of NAT10 expression. This increase facilitated proliferation, the accumulation of collagens, as well as fibroblast-to-myofibroblast transition. Through the administration of Remodelin, we effectively reduced cardiac fibrosis in myocardial infarction mice by inhibiting NAT10's ability to acetylate mRNA. Inhibition of NAT10 resulted in changes in the collagen-related gene expression and ac4C acetylation levels. Mechanistically, we identified that the inhibition of NAT10 mRNA ac4C modification activated caspase-3 and promoted cardiac fibroblast apoptosis. Therefore, the crucial involvement of NAT10-mediated ac4C acetylation is significant in the cardiac fibrosis progression, affording promising molecular targets for the treatment of fibrosis and relevant cardiac diseases.
Analyzing Factors that Influence Depression and Cognitive Dysfunction Following Cerebral Hemorrhage Surgery
Authors: Wen Bin , Zhang Ping , Niu Xiang , Cheng Hangtian , Li Zhong , Zhang Mingjie , Zhao Ziyu Y 
Published: August 2023

Abstract
Objective
This study aimed to identify the factors influencing depression and cognitive dysfunction in hypertensive patients after surgery for cerebral haemorrhage.
Methods
A total of 94 patients who underwent surgery were included, and their demographic information, pre- and post-surgical haemorrhagic data, and psychological assessments were collected.
Results
At 2 weeks post-surgery, the HAMD score for the right Basal Ganglia Haemorrhage (BGH) group was significantly higher than that of the right Basal Ganglia Breaking into Ventricular Haemorrhage (BGBVH), Ventricular Infarction and Haemorrhage (VIH), or Cerebellar Haemorrhage (CLH) groups (all P < 0.05). At 3 months, there was a significant difference in HAMD score between the high-risk right BGH and the low-risk VIH groups (P = 0.023). There was a correlation between Functional Independence Measure (FMA) ,Activities of Daily Living (ADL) and HAMD scores, as well as a linear relationship between Glasgow Coma Scale (GCS)/ADL scores and Mini-Mental State Examination (MMSE) scores, and a linear relationship between haemorrhage location, ADL score, and Montreal Cognitive Assessment (MoCA) scores.
Conclusion
The primary factor contributing to depression in patients with intracerebral haemorrhage (ICH) is the decrease in ADL and FMA scores after surgery. Additionally, ADL, GCS, surgical methods and bleeding sites can affect the cognitive function of patients.
Lifestyle profile is independently associated with incidence and mortality of nonalcoholic fatty liver disease
Authors: Tu Beilin , Li Wei , Xiao Haitao , Xu Xuewen , Zhang Yange 
Published: August 2023

Abstract
The aim of this study was to evaluate the association between lifestyle profile and disease incidence/mortality in patients with non-alcoholic fatty liver disease (NAFLD). Lifestyle profiles ascertainment was based on the latent profile analysis (LPA). The associations of lifestyle profile and outcomes were analyzed by multivariate logistic or Cox regressions. Four lifestyle profiles (profile 1 and 2 for male, profile 3 and 4 for female) were established for all participants. Compared to profile 1, profile 2 (P = 0.042) and profile 3 (P = 0.013) had lower incidence for NAFLD. In contrast, profile 4 showed similar NAFLD prevalence compared to profile 1 (P = 0.756). Individuals with NAFLD within profile 3 had the best long-term survival, and the HR was 0.55 (95% CI, 0.40–0.76) for all-cause mortality (compared to profile 1). Profile 4 (P = 0.098) and profile 2 (P = 0.546) had similar all-cause survival compared to profile 1. We explored the associations of healthy lifestyle score with mortality and incidence of NAFLD stratified by lifestyle profiles. We observed that with the increase of healthy lifestyle score, participants within profile 2 did not display lower NAFLD incidence and better long-term survival in NAFLD cases. In this study, lifestyle profiles were constructed in NHANES participants. The distinct lifestyle profiles may help optimize decision-making regarding lifestyle management in preventing NAFLD development, as well as selection of a more personalized approach for improving NAFLD survival.
Simultaneous evaluation ochratoxin A, aflatoxins, and zearalenone contamination level in Iran’s frijoles during years 2019-2022 using HPLC-FLD
Authors: kardani fatemeh , jelyani Aniseh zarei , Hashemi Mohammad , Rashedinia Marzieh , Shariati Saeedeh , Mahdavinia Masoud , Noori Seyyed Mohammad Ali 
Published: August 2023

Abstract
Considering the importance of frijoles in Iran as a favorite and widely consumed food, for the first time, research was conducted on the contamination of frijoles with mycotoxins. Mycotoxins were measured with a recovery of 95–102% by high-performance liquid chromatography (HPLC) and extracted and purified using a conventional commercial immunoaffinity column. In this research, 450 samples of frijoles, including 150 beans, 150 lentils, and 150 peas were examined. All the beans and peas were free of mycotoxins, and only 5 lentil samples contained mycotoxins. Two samples were contaminated with Aflatoxin B2 (AFB2) and (Aflatoxin G1) AFG1 toxins, one sample was contaminated with Ochratoxin A (OTA), one sample was contaminated with (Zearalenone) ZEA and another sample was contaminated with AFG1) was identified, the value was detected 14.2 ± 0.16 ng/g and < LOQ, which was lower than the detectable level in Iran and the European Union. The results in this research show the improvement of the sanitary conditions of cultivation, harvesting, and storage after the harvesting of frijoles in Iran.
Analysis m6A-related genes of peripheral white blood cell in spinal cord injury as potential targets for prognosis and treatment
Authors: Wang Qian , Luo Mingran 
Published: August 2023

Abstract
Spinal cord injury (SCI) is a destructive neurological and pathological state that causes major motor, sensory and autonomic dysfunctions. N6-methyladenosine (m6A) is a reversible RNA modification and has been implicated in various biological processes. However, there are few studies on m6A in SCI. This project mainly explores the prognostic value of m6A-related genes as potential biomarkers in SCI, in order to establish a set of accurate diagnosis and prognostic prediction model. In this study, we used GSE151371 dataset in GEO database including 38 SCI and 10 healthy samples to screen out 289 SCI related candidate genes and 5 m6A-related candidate genes with high SCI correlation and high differential expression through differentially expressed gene analysis, WGCNA and m6A correlation analysis. These genes are involved in inflammatory response, negative regulation of immune system process and molecular function of carbohydrate binding, iron ion binding, organic acid binding, long-chain fatty acid binding and so on. Furthermore, the key genes were evaluated through a protein-protein interaction (PPI) network combined with a co-expression network. The genes associated with the prognosis of SCI were screened out by MRI related analysis. The comparatively higher expression of PPARG in WBC correlates with worse prognosis in SCI patients, while lower expression of AK5 predicts worse prognosis. Our integrated analysis illustrated the hub genes involved in SCI, shedding light on the development of prognostic markers. Further understanding of the function of the identified SCI hub genes could provide deep insights into the molecular mechanisms of SCI.
Genetic Associations between Autoimmune Diseases and the Risks of Sepsis and 28-day Mortality in critical care : A Two-Sample Mendelian Randomization Study
Authors: Tie Xin , Zhao Yanjie , Su Jing , Liu Xing , Zou Tongjuan , Yin Wanhong 
Published: August 2023

Abstract
Background
Sepsis is a prominent reason for admission in the Intensive Care Unit (ICU), where certain autoimmune diseases exhibit dysregulation of cytokines similar in sepsis. Existing research suggests that individuals with autoimmune disorders are more susceptible to developing sepsis and experiencing higher mortality rates. This highlights the need for more precise strategies. However, current observational studies provide conflicting conclusions regarding the relationship between autoimmune conditions and sepsis. Therefore, we utilize the Mendelian randomization(MR) to further investigate this association.
Methods
We conducted a two-sample MR study in European population to assess causal relationships between autoimmune diseases and sepsis, we employed the inverse variance-weighted (IVW) method and used Cochran's Q test for heterogeneity. We performed MR Egger intercept and MR pleiotropy residual sum and outlier (MR-PRESSO) global test to test for potential imbalanced pleiotropy.
Results
Genetically predicted Crohn's disease (β = 0.067, se = 0.034, p = 0.046, OR = 1.069, 95% CI = 1.001–1.141) and idiopathic thrombocytopenic purpura (β = 0.069, se = 0.031, p = 0.023, OR = 1.071, 95% CI = 1.009–1.136) were positively associated with an increased risk of sepsis in critical care. Conversely, rheumatoid arthritis (β=-0.104, se = 0.047, p = 0.025, OR = 0.901, 95% CI = 0.823–0.987), ulcerative colitis (β=-0.208, se = 0.084, p = 0.013, OR = 0.812, 95% CI = 0.690–0.957), and narcolepsy (β=-0.202, se = 0.092, p = 0.028, OR = 0.818, 95% CI = 0.684–0.978) were associated with a reduced risk of sepsis in critical care. Moreover, Crohn's disease (β = 0.234, se = 0.067, p = 0.001, OR = 1.263, 95% CI = 1.108–1.440) and idiopathic thrombocytopenic purpura (β = 0.158, se = 0.061, p = 0.009, OR = 1.171, 95% CI = 1.041–1.317) were also linked to an increased risk of 28-day mortality of sepsis in critical care. In contrast, multiple sclerosis (β=-0.261, se = 0.112, p = 0.020, OR = 0.771, 95% CI = 0.619—0.960) and narcolepsy (β=-0.536, se = 0.184, p = 0.003, OR = 0.585, 95% CI = 0.408—0.838) were linked to a decreased risk of 28-day mortality of sepsis in critical care.
Conclusion
This MR study identified causal associations between certain autoimmune diseases and risks of sepsis in critical care, and 28-day mortality in the European population. These findings provide us with a more refined approach to preventing the incidence of sepsis among individuals afflicted with autoimmune diseases. Additionally, exploring the underlying mechanisms of autoimmune diseases may potentially yield innovative approaches to diagnosing and treating sepsis.