bluuebunny/crossref_metadata_2025_split huggingface dataset:
From sum of two squares to arithmetic Siegel–Weil formulas
Authors: Li Chao 
Published: January 2023

The main goal of this expository article is to survey recent progress on the arithmetic Siegel–Weil formula and its applications. We begin with the classical sum of two squares problem and put it in the context of the Siegel–Weil formula. We then motivate the geometric and arithmetic Siegel–Weil formula using the classical example of the product of modular curves. After explaining the recent result on the arithmetic Siegel–Weil formula for Shimura varieties of arbitrary dimension, we discuss some aspects of the proof and its application to the arithmetic inner product formula and the Beilinson–Bloch conjecture. Rather than being intended as a complete survey of this vast field, this article focuses more on examples and background to provide easier access to several recent works by the author with W. Zhang and Y. Liu.
Multimodal Analyses of the Aging Forehead and Their Clinical Implications
Authors: Alfertshofer Michael , Engerer Nina , Frank Konstantin , Moellhoff Nicholas , Freytag David L , Cotofana Sebastian 
Published: January 2023

Abstract

Background
Recent research has indicated that in the upper face a delicate arrangement exists between the muscles of facial expression and the skin. This arrangement allows for immediate transmission of movements following muscle contraction, resulting in skin movements and skin rhytid formation.

Objectives
To investigate age-related changes of the biomechanical unit formed by facial muscles, their connective tissue envelope, and the skin of the upper face.

Methods
A total of 76 Caucasian volunteers (30 males, 46 females) with a mean age of 42.2 years (SD 18.6) and a mean body mass index of 24.58 kg/m2 (SD 3.7) were investigated. Three upper facial regions were analyzed for skin firmness and elasticity with cutometric assessment, vertical and horizontal skin vector displacement using 3-dimensional imaging, and muscle activity with surface-derived electromyography.

Results
Study participants of older age (&gt;42.2 years), when compared with younger participants (≤42.2 years), showed increased skin firmness, at 0.20 mm vs 0.30 mm (P &lt; .001); decreased skin elasticity at 53.2% vs 69.0% (P &lt; .001); increased vertical (not horizontal) skin mobility at 3.56 mm vs 1.35 mm (P &lt; .001); and decreased surface-derived electromyography (sEMG) signal of the frontalis muscle with 174 µV vs 309 µV (P = .039).

Conclusions
This study reveals that age-related changes occur in each component of the biomechanical unit formed by facial muscles, connective tissue envelope, and skin in the upper face. Knowledge and understanding of such changes can allow for more targeted and individualized surgical and nonsurgical aesthetic treatments.
Sialyl Lewis X mediates interleukin-1 beta-induced trophoblast adhesion to endometrial cells during human embryo implantation
Authors: Ma Zhi , Kessler Mirjana , Chelariu-Raicu Anca , Sperandio Markus , Mahner Sven , Jeschke Udo , von Schönfeldt Viktoria 
Published: January 2023

AbstractCell surface carbohydrate antigens sialyl Lewis X (sLeX) and Lewis Y (LeY) are paramount glycoconjugates and are abundantly expressed in the receptive endometrium. Furthermore, among the important biological functions of both antigens is their role in leukocytes adhesion and extravasation. Interleukin-1 beta (IL-1β) is involved in the process of human embryo implantation and placenta development. Here, we used an in vitro model to investigate whether sLeX and LeY are playing a role in the embryo implantation process mediated by IL-1β. Our results are showing that the expression of cell surface sLeX was enhanced in endometrial RL95-2 cells after exposure to IL-1β. RT-qPCR detection indicated that the transcript level of glycosyltransferase gene fucosyltransferase 3 (FUT3) was significantly elevated and that of FUT4/7 and ST3 beta-galactoside alpha-2,3-sialyltransferase 3/4 (ST3GAL3/4) were decreased by treatment with IL-1β. Modulatory role of glycosyltransferase FUT3 on sLeX biosynthesis was determined by FUT3 siRNA transfection in RL95-2 cells. Results showed that the expression level of sLeX was suppressed, but no change was observed in regard to LeY. Moreover, IL-1β promoted the HTR-8/SVneo trophoblast spheroids attachment to the RL95-2 endometrial monolayer, which was partially blocked by anti-sLeX antibody and FUT3 knockdown. Gene expression analysis of the RNA-seq transcriptome data from human secretory endometrium demonstrated a significantly higher level of FUT3 in the mid-secretory phase compared to the early secretory phase, which was correlated with the expression of IL1B. In summary, the inflammatory microenvironment at the fetomaternal interface can regulate the glycosylation pattern of endometrial cells at the time of implantation. SLeX can be significantly induced by IL-1β via increasing FUT3 expression, which facilitates the trophoblast adhesion during embryo implantation.
Elevated insulin growth factor-1 in dentate gyrus induces cognitive deficits in pre-term newborns
Authors: Sharma Deep R , Cheng Bokun , Jaiswal Manoj Kumar , Zhang Xusheng , Kumar Ajeet , Parikh Nirzar , Singh Divya , Sheth Hardik , Varghese Merina , Dobrenis Kostantin , Zhang Xiaolei , Hof Patrick R , Stanton Patric K , Ballabh Praveen 
Published: January 2023

Abstract
Prematurely born infants are deprived of maternal hormones and cared for in the stressful environment of Neonatal Intensive Care Units (NICUs). They suffer from long-lasting deficits in learning and memory. Here, we show that prematurity and associated neonatal stress disrupt dentate gyrus (DG) development and induce long-term cognitive deficits and that these effects are mediated by insulin growth factor-1 (IGF1). Nonmaternal care of premature rabbits increased the number of granule cells and interneurons and reduced neurogenesis, suggesting accelerated premature maturation of DG. However, the density of glutamatergic synapses, mature dendritic spines, and synaptic transmission were reduced in preterm kits compared with full-term controls, indicating that premature synaptic maturation was abnormal. These findings were consistent with cognitive deficits observed in premature rabbits and appeared to be driven by transcriptomic changes in the granule cells. Preterm kits displayed reduced weight, elevated serum cortisol and growth hormone, and higher IGF1 expression in the liver and DG relative to full-term controls. Importantly, blocking IGF-1 receptor in premature kits restored cognitive deficits, increased the density of glutamatergic puncta, and rescued NR2B and PSD95 levels in the DG. Hence, IGF1 inhibition alleviates prematurity-induced cognitive dysfunction and synaptic changes in the DG through modulation of NR2B and PSD95. The study identifies a novel strategy to potentially rescue DG maldevelopment and cognitive dysfunction in premature infants under stress in NICUs.
Clinical Perspective on Use of Long-Read Sequencing in Prenatal Diagnosis of Thalassemia
Authors: Toledo Diana M , Lafferty Katherine A 
Published: January 2023

AbstractThis is an editorial focusing on the clinical perspective of a long-read sequencing method in the prenatal diagnosis of alpha- and beta-thalassemia, including a comparison between this method and standard PCR-based methods. Though incremental, the increased sensitivity and specificity using long-read sequencing is an important advantage of this methodology in the prenatal diagnostic arena due to false positive or false negative results having greater consequence when a family is making decisions about their pregnancy.
Constructing recombinantSaccharomyces cerevisiaestrains for malic-to-fumaric acid conversion
Authors: Steyn Annica , Viljoen-Bloom Marinda , Van Zyl Willem Heber 
Published: January 2023

AbstractSaccharomyces cerevisiae with its robustness and good acid tolerance, is an attractive candidate for use in various industries, including waste-based biorefineries where a high-value organic acid is produced, such as fumaric acid could be beneficial. However, this yeast is not a natural producer of dicarboxylic acids, and genetic engineering of S. cerevisiae strains is required to achieve this outcome. Disruption of the natural FUM1 gene and the recombinant expression of fumarase and malate transporter genes improved the malic acid-to-fumaric acid conversion by engineered S. cerevisiae strains. The efficacy of the strains was significantly influenced by the source of the fumarase gene (yeast versus bacterial), the presence of the XYNSEC signal secretion signal and the available oxygen in synthetic media cultivations. The ΔFUM1Ckr_fum + mae1 and ΔFUM1(ss)Ckr_fum + mae1 strains converted extracellular malic acid into 0.98 and 1.11 g/L fumaric acid under aerobic conditions.
Limosilactobacillus walteri sp. nov., a novel probiotic antimicrobial lipopeptide-producing bacterium
Authors: Saroha Tanu , Sharma Shikha , Choksket Stanzin , Korpole Suresh , Patil Prabhu B 
Published: January 2023

Abstract
A Gram-positive facultative anaerobe, nonspore forming, and nonmotile bacterial strain M31 was isolated from faecal contaminated soil. The strain is previously reported to produce a novel antimicrobial lipopeptide and displayed probiotic properties. The strain M31 is catalase negative and fermented d-galactose, d-glucose, esculin, d-maltose, d-lactose, d-melibiose, d-raffinose, d-saccharose (weak reaction), d-xylose (weak reaction), d-ribose (weak reaction), and l-arabinose (weak reaction). The majority of fatty acids were C16:0 (53.9%), C18:0 (26.9%), and C19:0 cyclo ω8c (19.1%). The genome is 2 234 040 bp long with 38.81% guanine-cytosine (GC) content. The pairwise ortho average nucleotide identity and digital DNA-DNA hybridization values of strain M31 with its closest relative species from Limosilactobacillus reuteri clade and Lm. rudii is below the recommended cut-off of 95% and 70%, respectively. Herein, we propose Lm. walteri sp. nov. as a novel species of the genus Limosilactobacillus with M31 = MTCC 12838 = JCM 32759 = KCTC 25569.
A cohort-based nutrition ECHO for community health workers
Authors: T. Thomas Karmella , Friedman Sarah A , J. Larson Madalyn , C. Jorgensen Troy , Sharma Sneha , Smith Amie , S. Lavi Mordechai 
Published: January 2023

AbstractProject Extension for Community Healthcare Outcomes (ECHO) Nevada applied the ECHO virtual hub-and-spoke telementoring model over nine 6-week cohorts (between November 2019 and November 2021) supporting community health workers (CHWs) who advise clients with diabetes or pre-diabetes. This study describes the program implementation, including evaluation data collection efforts. Didactic topics included ‘Intro to Healthy Eating and Easy Wins’ to ‘Grocery Shopping, Cooking Tips, Reading Labels, Meal Plans’ and ‘Reducing Bias and Being a Good Role Model’. Spoke participants signed up to review cases. Seventy-three of the enrolled participants (n = 100) attended three or more of the six sessions. Spoke participants completed 42 case presentations. The average self-efficacy increased from 2.7 [standard deviation (SD): 1.1] before completing the program to 4.1 (SD: 0.8) after completing the program. Average knowledge scores increased from 71 (SD: 16) before completing the program to 83 (SD: 14) after completing the program. Five group interviews drew actionable feedback that was incorporated into the program. Key elements of the ECHO model were successfully incorporated to support educational goals of a cohort of CHWs in nutritional coaching. Our program evaluation data tracking system shows non-significant but encouraging results regarding self-efficacy improvement and knowledge retention.
Predicting brain-regional gene regulatory networks from multi-omics for Alzheimer’s disease phenotypes and Covid-19 severity
Authors: Khullar Saniya , Wang Daifeng 
Published: January 2023

Abstract
Neuroinflammation and immune dysregulation play a key role in Alzheimer’s disease (AD) and are also associated with severe Covid-19 and neurological symptoms. Also, genome-wide association studies found many risk single nucleotide polymorphisms (SNPs) for AD and Covid-19. However, our understanding of underlying gene regulatory mechanisms from risk SNPs to AD, Covid-19 and phenotypes is still limited. To this end, we performed an integrative multi-omics analysis to predict gene regulatory networks for major brain regions from population data in AD. Our networks linked transcription factors (TFs) to TF binding sites (TFBSs) on regulatory elements to target genes. Comparative network analyses revealed cross-region-conserved and region-specific regulatory networks, in which many immunological genes are present. Furthermore, we identified a list of AD–Covid genes using our networks involving known and Covid-19 genes. Our machine learning analysis prioritized 36 AD–Covid candidate genes for predicting Covid severity. Our independent validation analyses found that these genes outperform known genes for classifying Covid-19 severity and AD. Finally, we mapped genome-wide association study SNPs of AD and severe Covid that interrupt TFBSs on our regulatory networks, revealing potential mechanistic insights of those disease risk variants. Our analyses and results are open-source available, providing an AD–Covid functional genomic resource at the brain region level.
Are heat-killed probiotics more effective than live ones on colon length shortness, disease activity index, and the histological score of an inflammatory bowel disease-induced murine model? A meta-analysis
Authors: Poaty Ditengou Junior Isaac Celestin , Ahn Sung-Il , Chae Byungho , Choi Nag-Jin 
Published: January 2023

AbstractThis study was conducted to compare the efficiency of heat-killed and live probiotics against colon length shortness, disease activity index (DAI), and the histological score of an inflammatory bowel disease (IBD) via a meta-analysis. In February 2022, the eligible papers were collected from four databases (Google Scholar, PubMed, ScienceDirect, and Scopus). Using common- and random-effects models, the effect sizes were estimated throughout the standardized mean difference. Forty-three papers were recorded for our meta-analysis, and the heterogeneity of the effect sizes was determined with Cochran’s Q test, followed by meta-ANOVA and meta-regression analyses. The probiotics (live and heat-killed) had globally an improving or preventive effect on colon length shortness, DAI, and histological score. The sub-group analysis revealed that the heat-killed probiotics had statistically (P &gt; .05) the same improving effect on colon length shortness, DAI, and histological score as live probiotics. In conclusion, this study suggested that live and heat-killed probiotics had a similar impact on IBD symptoms investigated in this study. The present outcomes would be a good base for researchers willing to further compare the effects of live and heat-killed probiotics on IBD.
Prognostic awareness in Japanese patients with advanced cancer: a follow-up cohort study
Authors: Hiratsuka Yusuke , Oishi Takayuki , Miyashita Mitsunori , Morita Tatsuya , Mack Jennifer W , Imai Hiroo , Mori Takahiro , Sakayori Masato , Mori Masanori , Maeda Isseki , Hamano Jun , Ishioka Chikashi , Inoue Akira 
Published: January 2023

AbstractBackgroundPatients with advanced cancer have been reported to be more likely to receive goal-concordant care if they have accurate prognostic awareness. However, many patients do not have this awareness. This study aimed to examine the prognostic awareness among Japanese patients with advanced cancer.MethodsThis single-center, follow-up cohort study included Japanese patients with advanced cancer who received chemotherapy at Tohoku University Hospital between January 2015 and January 2016. Patients were surveyed at enrollment and followed up for clinical events for 5 years thereafter. We compared (i) the patients’ prognostic awareness with both actual survival time and physician’s prediction of survival and (ii) physician’s prediction of survival time with actual survival. Factors associated with accurate prognostic awareness were identified by univariate analysis.ResultsOf the 133 patients eligible for the study, 57 patients were analyzed. Only 10 (17.5%) patients had accurate prognostic awareness. Forty-three patients (75.4%) were optimistic about their prognosis; &gt;80% of patients were more optimistic than their physicians about their prognosis. The physicians’ predictions were accurate in for patients (37.5%). Accurate prognostic awareness was associated with physician’s explanation of the prognosis and patients’ perception of a good death.ConclusionsA majority of the patients with advanced cancer in this study had prognostic awareness that was more optimistic in comparison with their actual survival, and most were more optimistic than their physicians about their prognosis. Further research is needed to develop programs to facilitate the discussion of life expectancy with patients in a manner that is consistent with their preferences.
Integration of chromatin accessibility and gene expression reveals new regulators of cold hardening to enhance freezing tolerance inPrunus mume
Authors: Li Ping , Zheng Tangchun , Li Lulu , Liu Weichao , Qiu Like , Ahmad Sagheer , Wang Jia , Cheng Tangren , Zhang Qixiang 
Published: January 2023

AbstractLow temperature is one of the most important abiotic factors limiting the growth, development and geographical distribution of plants. Prunus mume is an attractive woody ornamental plant that blooms in early spring in Beijing. However, the molecular mechanisms underlying cold hardening to enhance freezing tolerance in Prunus genus remains elusive. This study examined the dynamic physiological responses induced by cold hardening, and identified freezing-tolerance genes by RNA-seq and ATAC-seq analyses. Cold hardening elevated the content of soluble substances and enhanced freezing resistance in P. mume. Transcriptome analysis indicated that the candidate differentially expressed genes (DEGs) were those enriched in Ca2+ signalling, mitogen-activated protein kinase (MAPK) cascade, abscisic acid signalling, and inducer of CBF expression 1 (ICE)-C-repeat binding factor (CBF) signalling pathways. The openness of gene chromatin positively correlated with the expression level of these genes. Thirteen motifs were identified in the open chromatin regions in the treatment group subjected to freezing after cold hardening. The chromatin opening of transcription start site at the proximal –177 region of cold-shock protein CS120-like (PmCSL) was markedly increased, while the expression level of PmCSL was significantly up-regulated. Overexpression of PmCSL in Arabidopsis significantly improved the freezing tolerance of transgenic plants. These findings provide new insights into the regulatory mechanism of freezing tolerance to improve breeding of cold-hardy P. mume plants.
Tracing of persistent Listeria monocytogenes contamination in ewe’s milk farm
Authors: Minarovičová Jana , Adriana Véghová , Zuzana Kubicová , Andrezál Michal , Hana Drahovská , Eva Kaclíková 
Published: January 2023

Abstract
Ewe’s milk farm production is permanently associated with the risk of contamination by pathogenic bacteria, including Listeria monocytogenes. In the present study, the prevalence and diversity of L. monocytogenes strains repeatedly isolated from tank ewe’s milk and the milking environment on a farm in Slovakia during a prolonged period were investigated to identify the source of potentially persistent contamination. A total of 140 samples along the milk production chain were collected during an 18-month period. From all these samples, 45 samples were found L. monocytogenes positive with 90.3% positivity of tank milk samples (28 positive samples from 31 analysed). Pulsed-field gel electrophoresis profiling resulted in strain discrimination into six profiles with one pulsotype (NS1) corresponding to MLST-ST14 being predominant. A total of 17 proportionally selected L. monocytogenes isolates, including 11 NS1/ST14 isolates, were subjected to whole genome sequencing. Resulted data were used to compare the genomes diversity and to confirm the persistent contamination when &lt;10 allelic differences threshold in cgMLST analysis was applied. The source of persistent contamination was localized inside the milking apparatus, probably in shelters that were very difficult to clean. Despite great efforts, the ewe’s milk contamination could not be eliminated during the reporting period.
Potential role of rumen bacteria in modulating milk production and composition of admixed dairy cows
Authors: Lilian Musembei , Rawlynce Bett , Charles Gachuiri , Felix Kibegwa 
Published: January 2023

AbstractCattle milk is an important food for the growing calf and humans because of its concentrated macro and micro nutrients. However, the quantity and quality of milk nutrients vary depending on several factors. This study evaluated the effect of dietary modification on rumen bacteria as well as the potential role of rumen bacteria in modifying milk production and composition profile. Using a 16S rRNA metagenomic approach, the study characterized the rumen bacterial community composition in four dairy cows and their milk production and composition. The results indicated that Bacteroidetes and Firmicutes were the dominant bacteria, totaling ∼83.7% of the rumen bacteria. An increase in concentrate proportion in diet led to an increase in the abundance of Bacteroidetes (P ≤ 0.05) and Proteobacteria while Firmicutes and Fibrobacter decreased. Milk production and composition were highly correlated with the abundance of various rumen bacterial members. Lentispaerae (P = 0.010), and Synergistetes (P = 0.011) exhibited a positive and significant correlation while Tenericutes (P = 0.009) showed a negative correlation with milk protein. Fusobacteria (P = 0.016) showed a negative correlation with milk lactose. Similarly, several genera showed correlations with milk parameters. The correlation between microbes with milk parameters implies that the bacterial community possesses the potential to influence milk production and composition.
What gets categorized counts: Controlled vocabularies, digital affordances, and the international digital humanities conference
Authors: Guiliano Jennifer , Estill Laura 
Published: January 2023

AbstractThis article explores how terms are incorporated into the conference submission and review process for the international digital humanities conference. This article provides an overview of the Alliance for Digital Humanities Organizations (ADHO) conference reviewing process and how the controlled vocabulary structures the review process. We show how expanding and rethinking the controlled vocabulary can impact the experience of those who submit, review, and attend the conference. We consider how ConfTool, the submission and reviewing portal used for the international digital humanities conference, processes the controlled vocabulary and algorithmically influences the review of submissions. Ultimately, we advocate for the ability to make intentional and careful changes to conference vocabularies including considering the adoption of a formal ontology. We also suggest that changes to the ConfTool algorithm are needed to ensure a diverse and equitable future for digital humanities.