bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastric hamartomatous inverted polyp: Report of three cases with a review of the endoscopic and clinicopathological features
Authors: Ohtsu Takuya , Takahashi Yu , Tokuhara Mitsuo , Tahara Tomomitsu , Ishida Mitsuaki , Miyasaka Chika , Tsuta Koji , Naganuma Makoto 
Published: January 2023

AbstractObjectivesA gastric hamartomatous inverted polyp (GHIP) is a rare submucosal tumor characterized histopathologically by a submucosal inverted proliferation of cystically dilated hyperplastic gastric glands. Only 42 GHIPs have been reported in English literature. Few GHIPs have been reported to accompany adenocarcinomas. We reported on three patients with a GHIP and reviewed the clinicopathological and endoscopic features of GHIPs.MethodsThis study included two men and one woman with a GHIP. The endoscopic, histopathological, and immunohistochemical features of the endoscopically resected specimens were analyzed. A gene mutation analysis was also performed.ResultsAll the tumors were located in the body of the stomach, with a median size of 20 mm. Two tumors were sessile, and the remaining tumor had a pedunculated appearance. The overlying mucosa mainly appeared normal but was reddish in one tumor. The histopathological examination of the tumors revealed a well‐circumscribed and lobular submucosal proliferation of cystically dilated hyperplastic glands. The immunohistochemical analysis revealed that the MUC5AC‐positive foveolar epithelium was located in the center, and MUC6‐positive pseudo‐pyloric or pepsinogen‐I and H+/K+ ATPase‐positive fundic‐type glands were located at the periphery of two tumors. No carcinomatous components were noted in any of the tumors. Moreover, no significant mutations in oncogenes or tumor suppressor genes were noted.ConclusionsOur review revealed that approximately three fourths of GHIP cases showed an submucosal tumor‐like feature, whereas endoscopic features, including the endoscopic ultrasonography findings, were not characteristic. Because an endoscopic diagnosis of a GHIP may be difficult, complete endoscopic resection may be required for a pathological diagnosis.
Range reexpansion after long stasis: Italian otters (Lutra lutra) at their northern edge
Authors: Marcelli Manlio , Striglioni Federico , Fusillo Romina 
Published: January 2023

AbstractSpecies range shifts and expansion are subjects of primary research interest in the context of climate warming and biological invasions. Few studies have focused on reexpansion of species that suffered severe declines. Here, we focused on population recovery of Eurasian otters (Lutra lutra) in Italy, first detected in 2003 after a southward range contraction. We modeled the rate of range expansion and occupancy at the northern expanding front (central Italy), to gain insights into the progress of recovery and mechanisms of reexpansion. We performed a field survey in 2021, which redefined the northern limit of distribution further north, in close proximity to the Gran Sasso National Park. Then we analyzed a time series (1985–2021) of distances of northernmost occurrences from the center of the 1985 range. Using segmented regression, we were able to identify a prolonged stasis of the northern range edge and a simultaneous increase in occupancy from 0.151 to 0.4. A breakpoint was estimated in 2006, after which the range expanded northwards at an average rate of 5.48 km/year. From 2006 to 2021, the overall northward shift was about 80 km. Occupancy continued to increase until 2019 and abruptly declined in 2021. These patterns suggest that the reexpansion of the range can be limited by low occupancy at the expanding front. As occupancy increases, long‐distance dispersal increases and then range expands. The low occupancy at the current distribution limit of otters may reflect a higher anthropogenic pressure on northern habitats, which could slow down the reexpansion process.
Nanostructured NiCu LDHs/polypyrrole nanotubes composite for nonenzymatic glucose sensing in human serum
Authors: Liu Rui , Zhang Sai , Wang Tianze , Hu Ningyan , Ma Xiao , Zhu Liande 
Published: January 2023

AbstractNanostructured NiCu layered double hydroxides (NiCu LDHs) are synthesized in situ on polypyrrole nanotubes through convenient co‐precipitation and hydrothermal synthesis. The nanostructured composite (NiCu LDHs/PPy) shows high electrocatalytic activities towards the glucose oxidation reaction in alkaline electrolyte so that a nonenzymatic glucose sensor is developed. It is demonstrated that the sensor offers a wide linear range from 1.5 μM to 1.0 mM with a high sensitivity of 525.8 μA mM−1 cm−2 and a low limit of detection of 66 nM (S/N = 3). The nonenzymatic sensor has been successfully applied to real blood samples for glucose monitoring with high accuracy.
Deletion induced splicing in RIC3 drives nicotinic acetylcholine receptor regulation with implications for endoplasmic reticulum stress in human astrocytes
Authors: Yadav Navneesh , Thelma B. K. 
Published: January 2023

AbstractNicotinic acetylcholine receptor (nAChR) dysregulation in astrocytes is reported in neurodegenerative disorders. Modulation of nAChRs through agonists confers protection to astrocytes from stress but regulation of chaperones involved in proteostasis with pathological implications is unclear. Resistance to inhibitors of cholinesterase 3 (RIC3), a potential chaperone of nAChRs is poorly studied in humans. We characterized RIC3 in astrocytes derived from an isogenic wild‐type and Cas9 edited “del” human iPSC line harboring a 25 bp homozygous deletion in exon2. Altered RIC3 transcript ratio due to deletion induced splicing and an unexpected gain of α7nAChR expression were observed in “del” astrocytes. Transcriptome analysis showed higher expression of neurotransmitter/G‐protein coupled receptors mediated by cAMP and calcium/calmodulin‐dependent kinase signaling with increased cytokines/glutamate secretion. Functional implications examined using tunicamycin induced ER stress in wild‐type astrocyte stress model showed cell cycle arrest, RIC3 upregulation, reduction in α7nAChR membrane levels but increased α4nAChR membrane expression. Conversely, tunicamycin‐treated “del” astrocytes showed a comparatively higher α4nAChR membrane expression and upsurged cAMP signaling. Furthermore, reduced expression of stress markers CHOP, phospho‐PERK and lowered XBP1 splicing in western blot and qPCR, validated by proteome‐based pathway analysis indicated lowered disease severity. Findings indicate (i) a complex RNA regulatory mechanism via exonic deletion induced splicing; (ii) RIC‐3 as a disordered protein having contrasting effects on co‐expressed nAChR subtypes under basal/stress conditions; and (iii) RIC3 as a potential drug target against ER stress in astrocytes for neurodegenerative/nicotine‐related brain disorders. Cellular rescue mechanism through deletion induced exon skipping may encourage ASO‐based therapies for tauopathies.
The role of fathers in children's emotion regulation development: A systematic review
Authors: Islamiah Nur , Breinholst Sonja , Walczak Monika A. , Esbjørn Barbara H. 
Published: January 2023

AbstractThis systematic review summarizes the results of 43 studies that explored the potential role of fathers in emotion regulation (ER) development in children. Following a tripartite model, this review investigates the paternal modelling of ER strategies, emotion‐related paternal parenting practices, father–child emotional climate, and fathers' characteristics, by identifying 16 specific themes of paternal factors that could play a role in the child's ER development. Results show that while a large number of studies investigated father–child emotional climate and fathers' characteristics and their association with children's ER, the effects of paternal modelling and the father's emotion‐related parenting practices on children's ER are still understudied. This review reveals that several factors—fathers' modelling of ER strategies; positive reactions and support in responding to their child's expression of emotions; better quality of the father–child relationship; higher father–child attachment security; and positive parenting in terms of sensitivity, engagement, and expressiveness—had significant associations with children's higher ER skills. Conversely, fathers' psychopathology and harsh parenting were associated with poorer ER skills in children.Highlights
This study summarized existing literature that explored the association between paternal factors and ER in children.
The review showed some evidence supporting the paternal role in children's ER development.
Fathers' role in the development of child's ER is most prominent in infancy and toddlerhood.
Most paternal factors significantly associated with a child's ER reflected previous findings examining maternal factors.
Label‐free high‐resolution white light quantitative phase nanoscopy system
Authors: Tayal Shilpa , Tiwari Shubham , Mehta Dalip Singh 
Published: January 2023

AbstractWe present a high‐resolution white light quantitative phase nanoscopy (WLQPN) system that can be utilized to visualize nanoparticles and subcellular features of the biological specimens. The five‐phase shifting technique, along with deconvolution, is adopted to obtain super‐resolution in phase imaging. The phase shifting technique can provide full detector resolution, making it beneficial as compared to the well‐known Fourier analysis method. The Fourier transform method requires minimum angle of , where is maximum achievable spatial frequency. It limits the highest achievable resolution to much below the actual diffraction limit of the system. Thus, to obtain a high‐resolution phase map of the biological specimen, a two‐step process is adopted. First, the phase map is recovered using the five‐phase shifting algorithm, with full detector spatial resolution. Second, the complex field is obtained from the recovered phase map and further processed using the Richardson Lucy total variation deconvolution algorithm to obtain super‐resolution phase images. The present technique was tested on 1951 USAF resolution chart, 200 nm polystyrene beads and Escherichia coli bacteria using a 50×, 0.55NA objective lens. The 200 nm polystyrene beads are visually resolvable and subcellular features of the E. coli bacteria are also observed, suggesting a significant improvement in the resolution.
Acoustic radiation force optical coherence elastography: A preliminary study on biomechanical properties of trabecular meshwork
Authors: Ai Sizhu , Zhang Yubao , Shi Gang , Wang Yidi , Liu Guo , Han Xiao , Zhao Yanzhi , Yang Hongwei , He Xingdao 
Published: January 2023

AbstractEvaluating biomechanical properties of trabecular meshwork (TM) is of great significance for understanding the mechanism of aqueous humor circulation and its relationship to some eye diseases such as glaucoma; however, there is almost no relevant study due to the lack of clinical measurement tool. In this paper, an acoustic radiation force optical coherence elastography (ARF‐OCE) system is developed with the advantages of noninvasive detection, high resolution, high sensitivity, and high‐speed imaging, by which elastic modulus of the porcine and human TMs is accurately quantified. As the first OCE imaging of TM, our study demonstrates that ARF‐OCE may be an effective approach to advance the research of diseases related to aqueous humor circulation.
Identification of faecal extracellular vesicles as novel biomarkers for the non‐invasive diagnosis and prognosis of colorectal cancer
Authors: Zhang Zhaowei , Liu Xuehui , Yang Xiaoqing , Jiang Ying , Li Ang , Cong Jiying , Li Yuwei , Xie Qinjian , Xu Chen , Liu Dingbin 
Published: January 2023

AbstractColorectal cancer (CRC) is one of the most common malignancies that is usually detected late in the clinic. The currently available diagnostic tools for CRC are either invasive or insensitive to early lesions due to the dearth of reliable biomarkers. In this study, we discovered that the extracellular vesicles (EVs) in the faeces of CRC patients can act as a potent biomarker for the non‐invasive diagnosis and prognosis of CRC. This finding is based on the identification of two transmembrane proteins—CD147 and A33—on faeces‐derived EVs (fEVs) that are intrinsically associated with CRC. The detection results show that the levels of CD147 and A33 on fEVs were upregulated in the CRC patients (n = 48), dramatically distinguishing them from the healthy donors (n = 16). The CD147/A33‐enriched EVs offer a clinical sensitivity of 89%, much higher than that (40%) of carcinoembryonic antigen (CEA), a clinically‐established serum biomarker for CRC diagnosis. In addition, the analysis of longitudinal faeces samples (n = 29) demonstrated that the CD147/A33‐enriched fEVs can be utilized to track the prognosis of CRC. Due to the high compliance of faeces‐based detection, the CD147/A33‐enriched fEVs could serve as new‐generation CRC biomarkers for large‐scale, non‐invasive CRC screening as well as real‐time monitoring of patient outcomes during clinical interventions.
Visual discrimination and inhibitory control deficits in mouse models of Down syndrome: A pilot study using rodent touchscreen technology
Authors: Siegel Ashley Emily , Bianchi Diana W. , Guedj Faycal 
Published: January 2023

AbstractSeveral non‐verbal cognitive and behavioral tests have been developed to assess learning deficits in humans with Down syndrome (DS). Here we used rodent touchscreen paradigms in adult male mice to investigate visual discrimination (VD) learning and inhibitory control in the Dp(16)1/Yey (C57BL/6J genetic background), Ts65Dn (mixed B6 X C3H genetic background) and Ts1Cje (C57BL/6J genetic background) mouse models of DS. Dp(16)1/Yey and Ts1Cje models did not exhibit motivation or learning deficits during early pre‐training, however, Ts1Cje mice showed a significant learning delay after the introduction of the incorrect stimulus (late pre‐training), suggesting prefrontal cortex defects in this model. Dp(16)1/Yey and Ts1Cje mice display learning deficits in VD but these deficits were more pronounced in the Dp(16)1/Yey model. Both models also exhibited compulsive behavior and abnormal cortical inhibitory control during Extinction compared to WT littermates. Finally, Ts65Dn mice outperformed WT littermates in pre‐training stages by initiating a significantly higher number of trials due to their hyperactive behavior. Both Ts65Dn and WT littermates showed poor performance during late pre‐training and were not tested in VD. These studies demonstrate significant learning deficits and compulsive behavior in the Ts1Cje and Dp(16)1/Yey mouse models of DS. They also demonstrate that the mouse genetic background (C57BL/6J vs. mixed B6 X C3H) and the absence of hyperactive behavior are key determinants of successful learning in touchscreen behavioral testing. These data will be used to select the mouse model that best mimics cognitive deficits in humans with DS and evaluate the effects of future therapeutic interventions.
Enhanced precipitation prediction using DEM‐based predictors and satellite imagery
Authors: Antal Alexandru , Dumitrescu Alexandru , Cheval Sorin , Guerreiro Pedro M. P. 
Published: January 2023

AbstractOver the last decades, climatic changes have triggered considerable impacts across the globe with detrimental effects on all ecosystems. Given the complexity of topography and climate, Romania is one of the most exposed countries in the South‐Eastern Europe to extreme hydrological events. As a consequence, the spatial distribution of precipitation is of greater importance for future analysis. This study examines the performance of Empirical Bayesian Kriging Regression Prediction (EBKRP) and Geographically Weighted Regression (GWR) to predict the spatial distribution of annual and seasonal precipitation over Romania. Twelve co‐variables derived from topography, and remote sensing products data were used to improve the prediction. The co‐variable selection process was performed prior to the regression model to reduce the complexity and processing time, using the Boruta Algorithm (BA), which is an innovative approach. The performance of BA was compared with Gini Coefficient. Our findings confirmed that 10 co‐variables were relevant to predict annual precipitation and nine for seasonal. The overall prediction of precipitation is more influenced by topography (altitude, slope, surface roughness) and the distance to marine bodies (Black Sea and Adriatic Sea). Cross‐validation and five statistical metrics were applied to assess the performance of the regression models. The results show similar spatial distribution pattern of precipitation, whereas the highest annual precipitation is found in the Carpathian Mountains (EBKRP: 1,399.2 mm, GWR: 1,249.7 mm) and the lowest in the lowlands (EBKRP: 355.9 mm, GWR: 346.8 mm). For seasons, both methods predicted the highest precipitation in summer and lowest in autumn. In all seasons, the precipitation is underestimated by both methods, however, for annual, only GWR does so. Our study revealed GWR as the best method to predict annual and seasonal precipitation over Romania, as it yielded the highest correlation coefficient Spearman (S), Pearson (P), determination coefficient (R2) and lowest mean absolute error (MAE) and root mean square error (RMSE).
The underestimation of spring precipitation over South China is caused by the weak simulations of the dynamic motion in CMIP6 models
Authors: Ai Yawen , Chen Huopo , Sun Jianqi 
Published: January 2023

AbstractThis study investigates the performance of the 23 CMIP6 models in simulating spring precipitation over China and preliminarily explores the reasons for the underestimation of spring precipitation over South China (SC). The results show that the CMIP6 models can generally capture spring precipitation over China but have distinct underestimations in SC. In addition to analysing the results of the multimodel ensemble mean (MME), based on the regional mean spring precipitation differences over SC, we also analyse five models with greater overestimated precipitation amounts composing the high‐value MME (H_MME) and five models with greater underestimated precipitation amounts composing the low‐value MME (L_MME) as comparisons. Moisture budget analysis reveals that the underestimated dynamic component of vertical moisture advection mainly causes the negative biases of spring precipitation over SC. Further analyses indicate that the underestimation of the intensity of the East Asian subtropical jet (EASJ) generally causes weakened secondary meridional circulation to the south of 30°N and moisture convergence over SC in CMIP6 models. Consequently, less moistened air would be lifted to high levels, providing an unfavourable background for the occurrence of spring precipitation and inducing underestimation compared with the observations. The modelling EASJ shows much stronger (weaker) H_MME (L_MME), which generally enhances (weakens) the dynamic component of the secondary circulation over SC, favourable for the increase (decrease) in spring precipitation.
The impact of free‐radical stabilization techniques on in vivo subsurface mechanical properties in highly cross‐linked polyethylene acetabular liners
Authors: Kump Jordan , Teeter Matthew G. , Matheson John , Klassen Robert , Lanting Brent A. , Decker Michael M. 
Published: January 2023

AbstractHighly cross‐linked polyethylene (HXLPE) for total hip arthroplasty was developed to improve wear resistance in vivo and associated complications in comparison to ultrahigh molecular weight polyethylene. This material typically goes through various free‐radical stabilization techniques by remelting, single‐annealing, or sequentially annealing the polyethylene to improve in vivo oxidation and wear properties. The purpose of this study is to determine if there is evidence of subsurface microhardness changes in retrieved HXLPE liner at the rim and articular subsurface after extended in vivo time that could be associated with oxidation and its effects on mechanical properties and implant integrity. Retrieved HXLPE liners were chosen based on peak subsurface Fourier transform infrared spectroscopy oxidation values. Each was mechanically tested for subsurface microhardness at both the rim and articular surface using a validated microindentation technique. Rim testing demonstrated a decrease in mechanical integrity that corresponded to higher subsurface oxidation values regardless of the free‐radical stabilization technique. At the articular surface, a decrease in mechanical integrity was observed near the surface corresponding to peak oxidation and Vicker's hardness, which decreased with increasing depths. This was found in all groups, with the exception of the single‐annealed liners, which demonstrated decreased mechanical integrity trends at greater depths between 1.0 and 2.0 mm. Our results suggest that subsurface mechanical properties do change in vivo for certain implants. Though it is likely that the mechanical failures are multifactorial, we have shown that mechanical property degradation of HXLPE liners does occur with long‐term in vivo exposure and should be considered a possible risk factor.
Thermal, structural, and spectroscopic characterization of an ionic [Cu(H2O)4](ReO4)2 compound in a wide temperature range
Authors: Hetmańczyk Joanna , Hetmańczyk Łukasz , Nitek Wojciech , Pawlukojć Andrzej 
Published: January 2023

AbstractWe present complementary investigations of water and anion dynamics in an ionic [Cu(H2O)4](ReO4)2. This compound has one reversible phase transition in the solid state upon heating and cooling at 247 and 224 K, respectively. The value of entropy change indicates a moderate degree of molecular dynamical disorder. At room temperature, tetraaquacopper(II) rhenate(VII) crystallizes in a triclinic crystal system, within the space group No. 2 = P‐1, with one molecule in the unit cell. X‐ray single crystal diffraction measurements indicate that the crystal structure does not change significantly at the phase transition. However, neutron diffraction experiments performed on the powdered sample revealed some subtle changes in the registered patterns. Vibrational–reorientational dynamics of H2O ligands in the high (I) and low‐temperature (II) phases of [Cu(H2O)4](ReO4)2 was probed by a set of complementary spectroscopy techniques in a wide temperature range. The dynamics of the H2O molecules in both phases was investigated by means of band shape analysis performed for infrared bands. The H2O ligands perform fast (τR ≈ 10−11 to 10−12 s) stochastic reorientational motions in the phases I and II with a mean value of activation energy: 6.13 kJ⋅mol−1. However, a continuous change of bands connected with hydrogen bonds is observed. The Raman measurements revealed that in the phase transition region the intensity of bands connected with perrhenate anion significantly increases. Using vibrational spectroscopy methods all characteristic wavenumbers of the H2O and ReO4− vibrations were detected.