bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global research trends on COVID-19 and stroke: A bibliometric analysis
Authors: Zeng Youjie , Cao Si , Yang Heng 
Published: April 2023

BackgroundThe pandemic of COVID-19 has had a profound influence on worldwide healthcare systems. Our study aimed to conduct a bibliometric analysis to explore the impact of COVID-19 on stroke and to highlight the major research trends in this field.MethodsWe searched the original articles and review articles regarding COVID-19 and stroke from the Web of Science collection (WOSCC) database between January 1, 2020 and December 30, 2022. Subsequently, we performed bibliometric analyses and visualization using VOSviewer, Citespace, and Scimago Graphica.ResultsA total of 608 original articles or review articles were included. JOURNAL OF STROKE and CEREBROVASCULAR DISEASES published the most studies on this subject (n = 76), while STROKE was the source of the most-cited references (n = 2,393). The United States is the most influential country in this field, with the highest number of publications (n = 223) and citations (n = 5,042). Shadi Yaghi from New York University is the most prolific author in the field, while Harvard Medical School is the most prolific institution. In addition, through keyword analysis and reference co-citation analysis, three major research topics were identified: (i) the impact of COVID-19 on stroke outcomes (including risk factors, clinical characteristics, mortality, stress, depression, comorbidities, etc.); (ii) the management and care of stroke patients during the COVID-19 pandemic (including thrombolysis, thrombectomy, telemedicine, anticoagulation, vaccination, etc.); and (iii) the potential relationship and pathological mechanism between COVID-19 and stroke (including renin-angiotensin system activation, SARS-CoV-2 virus-induced inflammation leading to endothelial impairment, coagulopathy, etc.).ConclusionOur bibliometric analysis provides a comprehensive overview of the current state of research on COVID-19 and stroke and highlights key areas of focus in the field. Optimizing the treatment of COVID-19-infected stroke patients and elucidating the underlying pathogenic mechanisms of COVID-19 and stroke co-morbidity are key areas of future research that will be beneficial in improving the prognosis of stroke patients during the ongoing COVID-19 epidemic.
A prediction nomogram for moderate-to-severe bronchopulmonary dysplasia in preterm infants < 32 weeks of gestation: A multicenter retrospective study
Authors: Zhang Jing , Mu Kai , Wei Lihua , Fan Chunyan , Zhang Rui , Wang Lingling 
Published: April 2023

BackgroundModerate-to-severe bronchopulmonary dysplasia (msBPD) is a serious complication in preterm infants. We aimed to develop a dynamic nomogram for early prediction of msBPD using perinatal factors in preterm infants born at &lt;32 weeks' gestation.MethodsThis multicenter retrospective study conducted at three hospitals in China between January 2017 and December 2021 included data on preterm infants with gestational age (GA) &lt; 32 weeks. All infants were randomly divided into training and validation cohorts (3:1 ratio). Variables were selected by Lasso regression. Multivariate logistic regression was used to build a dynamic nomogram to predict msBPD. The discrimination was verified by receiver operating characteristic curves. Hosmer-Lemeshow test and decision curve analysis (DCA) were used for evaluating calibration and clinical applicability.ResultsA total of 2,067 preterm infants. GA, Apgar 5-min score, small for gestational age (SGA), early onset sepsis, and duration of invasive ventilation were predictors for msBPD by Lasso regression. The area under the curve was 0.894 (95% CI 0.869–0.919) and 0.893 (95% CI 0.855–0.931) in training and validation cohorts. The Hosmer−Lemeshow test calculated P value of 0.059 showing a good fit of the nomogram. The DCA demonstrated significantly clinical benefit of the model in both cohorts. A dynamic nomogram predicting msBPD by perinatal days within postnatal day 7 is available at https://sdxxbxzz.shinyapps.io/BPDpredict/.ConclusionWe assessed the perinatal predictors of msBPD in preterm infants with GA &lt; 32 weeks and built a dynamic nomogram for early risk prediction, providing clinicians a visual tool for early identification of msBPD.
Phytochemical component and toxicological evaluation of purple sweet potato leaf extract in male Sprague–Dawley rats
Authors: Hisamuddin Ahmad Safiyyu’d-din Bin , Naomi Ruth , Bin Manan Khairul Aiman , Bahari Hasnah , Yazid Muhammad Dain , Othman Fezah , Embong Hashim , Hadizah Jumidil Siti , Hussain Mohd Khairi , Zakaria Zainul Amiruddin 
Published: April 2023

This study assessed the toxicity of lutein-rich purple sweet potato leaf (PSPL) extract in male Sprague–Dawley rats.Methods and study design: A total of 54 adult male Sprague–Dawley rats were used. For the acute toxicity study, three rats in the acute control group were fed 2,000 mg/kg of PSPL for 14 days. The subacute toxicity study included six rats each in four groups administered 50, 250, 500, or 1,000 mg/kg for 28 days and observed for further 14 days without treatment in the subacute control and subacute satellite groups. Changes in body weight; blood biochemistry; hematological parameters; relative organ weight; and histological sections of the heart, kidney, liver, pancreas, aorta, and retina were observed for signs of toxicity.Results: The gradual increase in weekly body weight, normal level full blood count, normal liver and kidney profile, relative organ weight, and histological sections of all stained organ tissue in the treated group compared with the acute, subacute, and satellite control groups demonstrated the absence of signs of toxicity.Conclusion: Lutein-rich PSPL extract shows no signs of toxicity up to 2,000 mg/kg/day.
Cationic lipid-assisted nanoparticles for simultaneous delivery of CD47 siRNA and R848 to promote antitumor immune responses
Authors: Li Simin , Chen Yichang , Ma Ruolin , Du Ye , Han Bing 
Published: April 2023

Graphical AbstractThe PEG-PLGA nanoparticles effectively delivered R848 and CD47 siRNA into tumor cells, resulting in simultaneous activation of DCs and downregulation of CD47 expression on tumor cells, thereby enhancing antitumor immune responses by T cells.
Identification and characterization of Dof genes in Cerasus humilis
Authors: Liu Weili , Ren Weichao , Liu Xiubo , He Lianqing , Qin Chen , Wang Panpan , Kong Lingyang , Li Yang , Liu Yunwei , Ma Wei 
Published: April 2023

IntroductionDof genes encode plant-specific transcription factors, which regulate various biological processes such as growth, development, and secondary metabolite accumulation.MethodsWe conducted whole-genome analysis of Chinese dwarf cherry (Cerasus humilis) to identify ChDof genes and characterize the structure, motif composition, cis-acting elements, chromosomal distribution, and collinearity of these genes as well as the physical and chemical properties, amino acid sequences, and phylogenetic evolution of the encoded proteins.ResultsThe results revealed the presence of 25 ChDof genes in C. humilis genome. All 25 ChDof genes could be divided into eight groups, and the members of the same group had similar motif arrangement and intron-exon structure. Promoter analysis showed that cis-acting elements responsive to abscisic acid, low temperature stress, and light were dominant. Transcriptome data revealed that most ChDof genes exhibited tissue-specific expression. Then, we performed by qRT-PCR to analyze the expression patterns of all 25 ChDof genes in fruit during storage. The results indicated that these genes exhibited different expression patterns, suggesting that they played an important role in fruit storage.DiscussionThe results of this study provide a basis for further investigation of the biological function of Dof genes in C. humilis fruit.
The impact of road traffic context on secondary task engagement while driving
Authors: Cuentas-Hernandez Sandra , Li Xiaomeng , King Mark J. , Oviedo-Trespalacios Oscar 
Published: April 2023

IntroductionDriver distraction has been recognized for a long time as a significant road safety issue. It has been consistently reported that drivers spend considerable time engaged in activities that are secondary to the driving task. The temporary diversion of attention from safety-critical driving tasks has often been associated with various adverse driving outcomes, from minor driving errors to serious motor vehicle crashes. This study explores the role of the driving context on a driver’s decision to engage in secondary activities non-critical to the driving task.MethodThe study utilises the Naturalistic Engagement in Secondary Tasks (NEST) dataset, a complementary dataset derived from the SHRP2 naturalistic dataset, the most extensive naturalistic study to date. An initial exploratory analysis is conducted to identify patterns of secondary task engagements in relation to context variables. Maximum likelihood Chi-square tests were applied to test for differences in engagement between types of driver distraction for the selected contextual variables. Pearson residual graphs were employed as a supplementary method to visually depict the residuals that constitute the chi-square statistic.Lastly, a two-step cluster analysis was conducted to identify common execution scenarios among secondary tasks.ResultsThe exploratory analysis revealed interesting behavioral trends among drivers, with higher engagement rates in right curves compared to left curves, while driving uphill compared to driving downhill, in low-density traffic scenarios compared to high-density traffic scenarios, and during afternoon periods compared to morning periods. Significant differences in engagement were found among secondary tasks in relation to locality, speed, and roadway design. The clustering analysis showed no significant associations between driving scenarios of similar characteristics and the type of secondary activity executed.DiscussionOverall, the findings confirm that the road traffic environment can influence how car drivers engage in distracted driving behavior.
Mediating effects of meaning in life on the relationship between family care, depression, and quality of life in Chinese older adults
Authors: Zhou Jing-Jing , Zhang Yu , Ren Qing-Zhuo , Li Ting , Lin Gui-Ding , Liao Min-Yi , Chen Shao-Hua , Tong Pei , Gao Yu-Lin 
Published: April 2023

BackgroundThe study explored sources of meaning in older adults and the action path among family care, meaning in life, quality of life, and depression.Materials and methodsWe investigated 627 older adults using the Sources of Meaning in Life Scale for the Elderly (SMSE), the Family Care Index (APGAR), the Center for Epidemiological Studies Depression Scale-10 (CES-D-10), and the EuroqOL-5 Dimensions (EQ-5D).ResultsScores categorized 454 older adults with good family function, 99 with moderate, and 47 with severe family dysfunction; 110 older adults had depression. The structural equation model showed that family care affected the quality of life and depression by influencing meaning, and depression had a significant negative effect on the quality of life (P &lt; 0.05). The model was a good fit for the data (χ2/df = 3.300, SRMR = 0.0291, GFI = 0.975, IFI = 0.971, TLI = 0.952, CFI = 0.971, RMSEA = 0.062).ConclusionMeaning in life is an intermediary factor that affects depression and quality of life in older adults. Family care had a significant positive impact on SMSE and a negative influence on depression. The SMSE effectively clarifies the sources of meaning in life and can be used to improve meaning and promote mental health in older adults.
Factors that provide protection against intimate partner physical violence among married adolescents in Bangladesh
Authors: Rahman Mizanur , Jamil Kanta , Nahar Quamrun , Chakraborty Nitai , Haider M. Moinuddin , Khan Shusmita 
Published: April 2023

BackgroundIntimate partner violence (IPV), and especially intimate partner physical violence (IPPV), perpetrated by husbands, and within adolescence marriage are pervasive in Bangladesh. Younger women are more vulnerable to IPPV.ObjectivesWe examined factors associated with IPPV experienced by married adolescents ages 15–19 and tested four hypotheses: (1) adolescent girls married to relatively older husbands, (2) adolescents living in extended families with parents or parents-in-law, (3) adolescents who are minimally controlled by husbands, and (4) adolescents who have a child after marriage are protective of IPPV.MethodsWe analyzed IPPV data from 1,846 married girls ages 15–19 obtained from a national adolescent survey conducted in 2019–20. IPPV is defined as the respondent having physical violence perpetrated by her husband at least once in the last 12 months. We implemented logistic regression models to test our hypotheses.ResultsSixteen percent of married adolescent girls experienced IPPV. Girls living with parents-in-law or parents had adjusted odds ratio (AOR) of 0.56 (p &lt; 0.001) of IPPV compared to those girls who lived with husband alone. Girls with husbands ages 21–25 years and 26 years or older had AORs of 0.45 (p &lt; 0.001) and 0.33 (p &lt; 0.001) of IPPV compared to those girls with their husband ages 20 and younger. Married adolescent girls who did not own a mobile phone (an indicator of spousal power dynamics) had an AOR of 1.39 (p &lt; 0.05) compared to those girls who had a phone. IPPV risk increases with an increased duration of marriage for those with no living children (p &lt; 0.001) but not for those with at least one living child; the risk was higher among those who had a child within the 1st year of marriage than those who had not yet had a child. At a duration of 4 years and longer, IPPV risk was higher among those with no living children than those with children.DiscussionFindings related to those living with parents-in-law or parents, girls married to relatively older boys/men, having the ability to communicate with outside world, and having a child are protective of IPPV in Bangladesh are new, to our knowledge. Strictly adhering to the law that requires men waiting until the age of 21 to marry can reduce married girls' risk of IPPV. Raising girls' legal marriage age can minimize adolescents' IPPV and other health risks associated with adolescent childbearing.
In-home environment and happiness among older adults in Thailand
Authors: Pekalee Alongkorn , Gray Rossarin Soottipong 
Published: April 2023

BackgroundThe fact that housing can play a critical role in maintaining the health and independence related to happiness of the older population has been studied in more developed countries. However, research on the effect of housing conditions on happiness is rare in less developed countries. This study aimed to construct and test a structural equation model describing the structural relationship among personal aspects (living alone and physical disability), in-home environment (sleeping place and toilet/bathroom), and happiness among older adults in Thailand.MethodThe data on the population age 75 years or over were extracted from the 2017 national Survey of Older Persons in Thailand (n = 7,829).ResultsThe median age of the sample population was 79. Almost 60 percent were women. The structural equation model showed a good fit with the data. Living alone did not directly influence happiness. Physical disability had a statistically significant negative direct effect on happiness. In-home environment not only had an impact on happiness directly, but also moderated the relationship between physical disability and happiness.ConclusionThe research suggested that interventions to improve happiness of older adults, particularly those with physical disability, should aim to adapt their housing, including sleeping place and toilet design.
“Ah, it's best not to mention that here:” Experiences of LGBTQ+ health professionals in (heteronormative) workplaces in Canada
Authors: Bizzeth Stephanie R. , Beagan Brenda L. 
Published: April 2023

IntroductionDespite human rights protections for lesbian, gay, bisexual, transgender, and queer (LGBTQ+) people, LGBTQ+ professionals may continue to experience discrimination working in heteronormative systems and spaces.MethodsIn this qualitative study 13 health professionals (nurses, occupational therapists, and physicians) from across Canada participated in in-depth qualitative interviews to explore their experiences with work-related microaggressions and heteronormativity.ResultsHeterosexist microaggressions from both patients/clients and colleagues were the norm, perpetuating and bolstered by heteronormative workplace and professional cultures. In turn, LGBTQ+ professionals navigated disclosure-decision-making, in power-laden contexts where all options carried potential negative consequences.DiscussionDrawing on the notion of “heteroprofessionalism,” we argue that the concept of professional carries encoded within it demands that the occupant of that category be—or present as—heterosexual, an unmarked status that can be readily desexualized. Acknowledging sex and sexuality disrupts “professionalism.” We argue that such disruption, indeed dissention, is necessary to open (hetero)professional spaces to LGBTQ+ workers.
Interdependencies of Gene Expression and Function between Two Redox Enzymes and REG Family Proteins in Murine Pancreatic Islets and Human Pancreatic Cells
Authors: Wang Hong , Vatamaniuk Marko Z. , Zhao Zeping , Lei Xin Gen 
Published: April 2023

Our laboratory previously revealed that regenerating islets-derived protein 2 (REG2) was diminished in pancreatic islets of glutathione peroxidase-1-overexpressing mice (Gpx1-OE). It remained unknown if there is an inverse relationship between the expression and function of all Reg family genes and antioxidant enzymes in the pancreatic islets or human pancreatic cells. This research was to determine how altering the Gpx1 and superoxide dismutase-1 (Sod1) genes alone or together (dKO) affected the expression of all seven murine Reg genes in murine pancreatic islets. In Experiment 1, Gpx1-/-, Gpx1-OE, their wild-type (WT), Sod1-/-, dKO, and their WT (male, 8-wk old, n = 4–6) were fed a Se-adequate diet and their islets were collected to assay the mRNA levels of Reg family genes. In Experiment 2, islets from the six groups of mice were treated with phosphate-buffered saline (PBS), REG2, or REG2 mutant protein (1 µg/mL), and/or GPX mimic (ebselen, 50 µM) and SOD mimic (copper [II] diisopropyl salicylate, CuDIPS, 10 µM) for 48 h before the proliferation assay using bromodeoxyuridine (BrdU). In Experiment 3, human pancreatic cells (PANC1) were treated with REG2 (1 µg/mL) and assayed for REG gene expression, GPX1 and SOD1 activities, viability, and responses to Ca2+. Compared with the WT, knockouts of Gpx1 and/or Sod1 up-regulated (p < 0.05) the mRNA levels of most of the murine Reg genes in islets whereas the Gpx1 overexpression down-regulated (p < 0.05) Reg mRNA levels. REG2, but not the REG2 mutant, inhibited islet proliferation in Gpx1 or Sod1-altered mice. Such inhibition was abolished by co-incubation the Gpx1-/- islets with ebselen and the Sod1-/- islets with CuDIPS. Treating PANC1 cells with murine REG2 protein induced expression of its human orthologue REG1B and three other REG genes, but decreased SOD1 and GPX1 activities and cell viability. In conclusion, our results revealed an interdependence of REG family gene expression and/or function on intracellular GPX1 and SOD1 activities in murine islets and human pancreatic cells.
A Screening Model of Antibacterial Agents Based on Escherichia coli Cell-Division Protein
Authors: Fan Qiuyu , Wu Jianwen , Xi Bolin , Li Chunxiao , Wang Xiumin , Li Huanrong 
Published: April 2023

Pathogenic Escherichia coli cannot be killed by most antibiotics (including colistin, a last-resort drug) due to the rapid development of antibiotic resistance. A highly conserved prokaryotic mitotic protein, filamenting temperature-sensitive protein Z (FtsZ) with GTPase activity, plays a key role in cell division and has become a promising target for screening novel antibacterial agents. In this study, the amplified ftsZ gene was inserted into cloning/expression vectors and recombinantly produced in E. coli; the recombinant FtsZ protein was purified by the Ni2+-NTA affinity column and then was used to screen for natural antibacterial agents. The results showed that the ftsZ gene with a size of 1170 bp was successfully amplified from E. coli and inserted into the pET-28a expression vector. After induction with 0.2 mM isopropyl β-D-1-thiogalactopyranoside (IPTG), FtsZ was expressed in E. coli BL21 as inclusion bodies. After purification, the recombinant FtsZ protein showed GTPase activity. The highest GTPase activity (0.998 nmol/mL/min) of FtsZ was observed at a GTP concentration of 1.25 mM. Several alkaloids were screened by a constructed model of FtsZ inhibitors. Sanguinarine chloride exhibited higher antibacterial activity against E. coli and Salmonella enteritidis (with minimum inhibitory concentrations (MICs) of 0.04–0.16 mg/mL and minimum bactericidal concentrations (MBCs) of 0.16–0.32 mg/mL) than tetrandrine (0.16–0.32 mg/mL) and berberine hydrochloride (0.32–0.64 mg/mL). Berberine hydrochloride prevented FtsZ polymerization in a concentration-dependent manner and bound to FtsZ protein by hydrogen bonding interaction. This study suggested that the FtsZ-based E. coli screening model could be exploited for the development of novel antibacterial agents for clinical applications.
Shipwrecks of Feasibility in the Field of Investment Business
Authors: Parashtheo Bintang , Cahyono Akhmad Budi 
Published: April 2023

Indonesian Valuable Objects on the Load of Sinking Ships (BMKT) have a large potential. This makes the ships buried in Indonesian territorial seas a tremendous resource. Illegal treasure hunters have targeted Indonesia's BMKT because to its great potential, causing considerable losses. Realizing this, it is vital to pay attention to Valuable Items on the Load of Sinking Ships (BMKT) and develop rules to maximize their potential. This study aims to analyze the policies governing BMKT to support investors in participating in investing in the business sector of BMKT management and creating BMKT management that is optimal and feasible for all aspects of the values contained in the BMKT itself. The approach method used to achieve this research is an approach focused on regulations or policies (statute approach) and conceptual analysis. The result showed that there is still substantial overlap between regulations controlling BMKT, making BMKT administration feasible. In spite of this, the Economic Analysis of Law is used to explain the expansion of the legal dimension through its studies. In addition, the economic value and cultural legacy of BMKTs that cannot be appointed due to the difficulties of appointment or the impossibility of relocation may be used on-site via marine tourist activities, notably diving tourism. As such, investors who wish to participate in the business sector of BMKT management may use BMKT more properly and optimally, including local and international investors.