bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of modified bladder neck suspension on early recovery of continence after robot-assisted radical prostatectomy (RARP)
Authors: Moon Hyong Woo , Rhew Seung Ah , Yoon Chang Eil , Kwon Hyeok Jae , Park Yong Hyun , Lee Ji Youl 
Published: June 2023

AbstractThe incontinence after RARP significantly decreases the quality of life in prostate cancer patients. A number of techniques have been introduced for the recovery of continence after RARP. Although, the mechanism of the continence recovery is still unclear. We aimed to evaluate the early recovery of continence after RARP by inducing early anterior adhesion and reducing the hypermobility of the urethra through the modified bladder neck suspension (BNS) procedure. From March 2018 to February 2020, a total of 227 consecutive patients who underwent RARP (by single surgeon) were included. Patients were divided into two groups based on operation procedure (Standard procedure vs BNS procedure). Demographics, perioperative variables, and pathologic outcome were analyzed. We assessed recovery of continence at 1, 3, 6 and 9 months after surgery. Postoperative recovery of continence defined as the use of no pad during 24 h. Multivariable logistic regression analyses were performed to evaluate independent predictors of the early recovery of continence at 1 month. We performed RARP with standard procedure (n = 106) or BNS procedure (n = 121). There was no statistical difference in perioperative variables between the two groups except anastomosis time (21.6 ± 12.9 vs 17.0 ± 7.6, p = 0.003). The pad free continence rate were 80.2% (standard group) and 91.3% (BNS group) at 9 month after RARP (p = 0.037). However, early continence rate (1mo) were significantly higher in the BNS group (12.3% vs 29.1%, p = 0.004). On multivariate logistic analyses, BNS procedure (odds ratio [OR] 2.78, 95% confidence interval [CI] 1.03–7.45, p = 0.0426), age (OR 0.92, CI 0.86–0.98, p = 0.0154) were independent factor for early recovery of continence after RARP. The modified bladder neck suspension procedure showed significantly better outcomes than the standard procedure in terms of the early recovery of urinary continence.
The Effect of Lanthanum (III) Nitrate on the Osteogenic Differentiation of Mice Bone Marrow Stromal Cells
Authors: Wang Qian , Li Yi-Fan , Zhang Hai-Song , Li Xue-Zhong , Gao Yan , Fan Xing 
Published: June 2023

AbstractTo study the species of lanthanum (III) nitrate (La[NO3]3) dispersed in cell media and the effect on the osteoblast differentiation of bone marrow stroma cells (BMSCs). Different La-containing precipitations were obtained by adding various concentrations of La(NO3)3 solutions to Dulbecco’s modified Eagle medium (DMEM) or DMEM with fetal bovine serum (FBS). A series of characterisation methods, including dynamic light scattering, scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and protein quantification were employed to clarify the species of the different La-containing precipitations. The primary BMSCs were isolated, and the cell viability, alkaline phosphatase activity, and the formation of a mineralised nodule of BMSCs were tested when treated with different La-containing precipitations. The La(NO3)3 solutions in DMEM could form LaPO4, which exits in the particle formation, while the La(NO3)3 solutions in DMEM with FBS could form a La-PO4-protein compound. When treated with La(NO3)3 solutions in DMEM, the cell viability of the BMSCs was inhibited at the concentrations of 1, 10, and 100 μM at 1 day and 3 days. Meanwhile, the supernatant derived from the La(NO3)3 solutions in DMEM did not affect the cell viability of the BMSCs. In addition, the precipitate derived from the La(NO3)3 solutions in DMEM added to the complete medium inhibited the cell viability of the BMSCs at concentrations of 10 μM and 100 μM. When treated with La(NO3)3 solutions in DMEM with FBS, the derived precipitate and supernatant did not affect the cell viability of the BMSCs, except for the concentration of 100 μM La(NO3)3. The La-PO4-protein formed from the La(NO3)3 solutions in DMEM with FBS inhibited the osteoblast differentiation of BMSCs at the concentration of 1 μM La(NO3)3 (P < 0.05) but had no effect on either the osteoblast differentiation at the concentrations of 0.001 and 0.1 μM or on the formation of a mineralised nodule at all tested concentrations of La(NO3)3. Overall, La(NO3)3 solutions in different cell culture media could form different La-containing compounds: La-PO4 particles (in DMEM) and a La-PO4-protein compound (in DMEM with FBS). The different La-containing compounds caused different effects on the cell viability, osteoblast differentiation, and the formation of a mineralised nodule of the BMSCs. The La-containing precipitation inhibited the osteoblast differentiation by inhibiting the expression of osteoblast-related genes and proteins, providing a theoretical basis for clinical doctors to apply phosphorus-lowering drugs such as lanthanum carbon.
Myoepithelial Carcinoma of Sinonasal Cavity: Peculiar Diagnosis, Conventional Treatment
Authors: Dinker Diksha , Rajan Keshava , Sharma Swati , Kumar Naveena AN 
Published: June 2023

AbstractMyoepithelial carcinoma is a morphologically diverse tumor which either arises de novo or from the malignant transformation of its benign counterpart i.e. myoepithelioma. These are relatively lesser known entities and are rarely found in head and neck region. Although rare, their first presentation is usually a painless growing mass as seen in our case presentation as well and are infamous for lymph node recurrence and distant metastasis. Due to their clinical presentation and varied morphology these become tedious to diagnose and pose difficulty for a surgeon when presented at a later date due to their effect on the adjacent vital structures. We report a case of myoepithelial carcinoma in head and neck region arising from the nasal cavity, it’s mass effect on the adjacent vital organs and the diagnosis and treatment plan to render the patient free of this tumor, preservation of the vision and keeping the recurrence of the tumor at bay.
Liprin-α1 Expression in Tumor-Infiltrating Lymphocytes Associates with Improved Survival in Patients with HPV-Positive Oropharyngeal Squamous Cell Carcinoma
Authors: Sjöblom Anni , Pehkonen Henna , Jouhi Lauri , Monni Outi , Randén-Brady Reija , Karhemo Piia-Riitta , Tarkkanen Jussi , Haglund Caj , Mattila Petri , Mäkitie Antti , Hagström Jaana , Carpén Timo 
Published: June 2023

Abstract
Background
Liprin-α1 is a scaffold protein involved in cell adhesion, motility, and invasion in malignancies. Liprin-α1 inhibits the expression of metastatic suppressor CD82 in cancers such as oral carcinoma, and the expression of these proteins has been known to correlate negatively. The role of these proteins has not been previously studied in human papillomavirus (HPV)-related head and neck cancers. Our aim was to assess the clinical and prognostic role of liprin-α1 and CD82 in HPV-positive oropharyngeal squamous cell carcinoma (OPSCC) in comparison to HPV-negative OPSCC.

Methods
The data included 139 OPSCC patients treated at the Helsinki University Hospital (HUS) during 2012–2016. Immunohistochemistry was utilized in HPV determination and in biomarker assays. Overall survival (OS) was used in the survival analysis.

Results
Stronger expression of liprin-α1 in tumor-infiltrating lymphocytes (TILs) was linked to lower cancer stage (p < 0.001) and HPV positivity (p < 0.001). Additionally, we found an association between elevated expression of liprin-α1 and weak expression of CD82 in tumor cells (p = 0.029). In survival analysis, we found significant correlation between favorable OS and stronger expression of liprin-α1 in TILs among the whole patient cohort (p < 0.001) and among HPV-positive patients (p = 0.042).

Conclusions
Increased liprin-α1 expression in the TILs is associated with favorable prognosis in OPSCC, especially among HPV-positive patients.
An updated description of Parkia barnebyana (Leguminosae: Caesalpinioideae, mimosoid clade) from Amazonian Venezuela and Brazil, and comparison with other species in sect. Parkia with erect capitula
Authors: Hopkins Helen C. F. , Oliveira Lorena C. , Hopkins Michael J. G. 
Published: June 2023

SummaryAn updated description, including characters of the capitulum and fertile flowers, is provided for Parkia barnebyana. Originally recorded from southern Venezuela, this small canopy tree from igapó (blackwater flooded forest) is now also known by two collections from north-western Brazil. The capitula are yellow and held erect, with the fertile flowers uppermost; they are borne on short, robust, upright peduncles that arise from long compound inflorescence axes projecting directly above the crown. The structure and shape of the capitula place this species in pantropical sect. Parkia and its pod morphology suggests that it lies outside the largely Amazonian nitida-group; both conclusions are supported by a recent phylogenetic analysis. The indehiscent pods remain in the tree at maturity and most seeds are apparently removed by arboreal dispersers and vertebrate seed predators. Two other neotropical species in sect. Parkia, P. cachimboensis and P. decussata, are known to have erect, yellow or largely yellow capitula and in both, a well-developed fringe of staminodia projecting from the basal flowers is absent. Both these species are chiropterophilous and although we predict that P. barnebyana is also likely to be bat-pollinated, we have made no observations. A fourth neotropical species, P. nana, also with yellow flowers, is reported to have erect capitula but they do have staminodial fringes; its pollination has not been studied. Within sect. Parkia, erect capitula occur only in the Neotropics, probably because of the head-down position in which quite large generalist phyllostomid bats land on them to access nectar. Erect capitula appear to have evolved more than once within neotropical sect. Parkia and at least sometimes from ancestors with pendent capitula, but further phylogenetic data are needed to confirm this.
Study of hydrogen sulfide biosynthesis in synovial tissue from diabetes-associated osteoarthritis and its influence on macrophage phenotype and abundance
Authors: Lendoiro-Cino Natalia , Rodríguez-Coello Arianna , Saborido Anna , F-Burguera Elena , Fernández-Rodríguez Jennifer A. , Meijide-Faílde Rosa , Blanco Francisco J. , Vaamonde-García Carlos 
Published: June 2023

Abstract
Type 2 diabetes (DB) is an independent risk factor for osteoarthritis (OA). However, the mechanisms underlying the connection between both diseases remain unclear. Synovial macrophages from OA patients with DB present a marked pro-inflammatory phenotype. Since hydrogen sulphide (H2S) has been previously described to be involved in macrophage polarization, in this study we examined H2S biosynthesis in synovial tissue from OA patients with DB, observing a reduction of H2S-synthetizing enzymes in this subset of individuals. To elucidate these findings, we detected that differentiated TPH-1 cells to macrophages exposed to high levels of glucose presented a lower expression of H2S-synthetizing enzymes and an increased inflammatory response to LPS, showing upregulated expression of markers associated with M1 phenotype (i.e., CD11c, CD86, iNOS, and IL-6) and reduced levels of those related to M2 fate (CD206 and CD163). The co-treatment of the cells with a slow-releasing H2S donor, GYY-4137, attenuated the expression of M1 markers, but failed to modulate the levels of M2 indicators. GYY-4137 also reduced HIF-1α expression and upregulated the protein levels of HO-1, suggesting their involvement in the anti-inflammatory effects of H2S induction. In addition, we observed that intraarticular administration of H2S donor attenuated synovial abundance of CD68+ cells, mainly macrophages, in an in vivo model of OA. Taken together, the findings of this study seem to reinforce the key role of H2S in the M1-like polarization of synovial macrophages associated to OA and specifically its metabolic phenotype, opening new therapeutic perspectives in the management of this pathology.
The D-amino acid oxidase-carbon nanotubes: evaluation of cytotoxicity and biocompatibility of a potential anticancer nanosystem
Authors: Rosini Elena , Boreggio Marta , Verga Matteo , Caldinelli Laura , Pollegioni Loredano , Fasoli Elisa 
Published: June 2023

AbstractThe ‘enzyme prodrug therapy’ represents a promising strategy to overcome limitations of current cancer treatments by the systemic administration of prodrugs, converted by a foreign enzyme into an active anticancer compound directly in tumor sites. One example is D-amino acid oxidase (DAAO), a dimeric flavoenzyme able to catalyze the oxidative deamination of D-amino acids with production of hydrogen peroxide, a reactive oxygen species (ROS), able to favor cancer cells death. A DAAO variant containing five aminoacidic substitutions (mDAAO) was demonstrated to possess a better therapeutic efficacy under low O2 concentration than wild-type DAAO (wtDAAO). Recently, aiming to design promising nanocarriers for DAAO, multi-walled carbon nanotubes (MWCNTs) were functionalized with polyethylene glycol (PEG) to reduce their tendency to aggregation and to improve their biocompatibility. Here, wtDAAO and mDAAO were adsorbed on PEGylated MWCNTs and their activity and cytotoxicity were tested. While PEG-MWCNTs-DAAOs have shown a higher activity than pristine MWCNTs-DAAO (independently on the DAAO variant used), PEG-MWCNTs-mDAAO showed a higher cytotoxicity than PEG-MWCNTs-wtDAAO at low O2 concentration. In order to evaluate the nanocarriers’ biocompatibility, PEG-MWCNTs-DAAOs were incubated in human serum and the composition of protein corona was investigated via nLC-MS/MS, aiming to characterize both soft and hard coronas. The mDAAO variant has influenced the bio-corona composition in both number of proteins and presence of opsonins and dysopsonins: notably, the soft corona of PEG-MWCNTs-mDAAO contained less proteins and was more enriched in proteins able to inhibit the immune response than PEG-MWCNTs-wtDAAO. Considering the obtained results, the PEGylated MWCNTs conjugated with the mDAAO variant seems a promising candidate for a selective antitumor oxidative therapy: under anoxic-like conditions, this novel drug delivery system showed a remarkable cytotoxic effect controlled by the substrate addition, against different tumor cell lines, and a bio-corona composition devoted to prolong its blood circulation time, thus improving the drug’s biodistribution.
Emulsion formation during enzymatic protein hydrolysis and its effect on protein recovery and molecular weight distribution of protein hydrolysates from sardine (Sardina pilchardus) by-products
Authors: Chiodza Kudzai , Goosen Neill J. 
Published: June 2023

AbstractRecovery of protein is one of the important variables to optimise enzymatic hydrolysis of fish processing by-products. This study investigated the role played by emulsion formation on protein recovery and the molecular weight distribution of the protein hydrolysates at different solids concentration of fish processing by-products with a high oil content. Solids concentration of fish processing by-products was varied between 26 and 50% at constant pH of 7.8, 60 °C hydrolysis temperature and 4% enzyme dosage using Alcalase 2.4L. The results showed that emulsion formation, more than inhibition of enzyme activity, at high solids concentration contributed to the reduction in yield of dry solids and protein recovery. Emulsion formation also led to the presence of high molecular weight lipo-protein complexes in the protein hydrolysates, which caused an increase in the average molecular weight of the hydrolysates. The findings show the negative relationship between solids concentration, protein recovery and emulsion formation in enzymatic hydrolysis, and the need for methods that eliminate or reduce emulsion formation at high solids concentration without increasing process complexity or reducing protein recovery.
Nonemptiness of severi varieties on enriques surfaces
Authors: Ciliberto Ciro , Dedieu Thomas , Galati Concettina , Knutsen Andreas Leopold 
Published: June 2023

Abstract
Let

$(S,L)$

be a general polarised Enriques surface, with L not numerically 2-divisible. We prove the existence of regular components of all Severi varieties of irreducible nodal curves in the linear system

$|L|$

, that is, for any number of nodes

$\delta =0, \ldots , p_a(L)-1$

. This solves a classical open problem and gives a positive answer to a recent conjecture of Pandharipande–Schmitt, under the additional condition of non-2-divisibility.
List constructions in two signed languages
Authors: Wilcox Sherman , Xavier André Nogueira , Siltaloppi Satu 
Published: June 2023

AbstractThis paper examines how signers make lists. One way is to use the fingers on the signer’s nondominant hand to enumerate items on a list. The signer points to these list-fingers with the dominant hand. Previous analyses considered lists to be nondominant, one-handed signs, and thus were called list buoys because the nondominant hand often remains in place during the production of the list. The pointing hand was largely ignored as a nonlinguistic gesture. We take a constructional approach based on Cognitive Grammar. In our approach, we analyze lists as a type of pointing construction consisting of two meaningful components: a pointing device (the pointing hand) used to direct attention; and a Place, also consisting of form and a meaning. Using data from Brazilian Sign Language (Libras) and Finland–Swedish Sign Language (FinSSL), we examine the semantic role of each component, showing how the nondominant list-fingers identify and track discourse referents, and how the pointing hand is used to create higher-order entities by grouping list-fingers. We also examine the integration of list constructions and their components with other conventional constructions.
A DRAWING EDUCATION PROGRAMME FOCUSING ON ENHANCING ABSTRACTION ABILITY
Authors: Yagisawa Masaki , Iijima Junichi 
Published: June 2023

AbstractThis study considers the 'three sub-abilities' that constitute the abstraction ability and focuses on drawing as an education for acquiring them. Focusing on the similarity between the process of drawing and the semiotic triangle, elucidating their relationship with the sub-abilities that constitute the abstraction ability, it devises a drawing education programme that focuses on 'observing' rather than 'drawing'. The drawing education programme formulated is implemented on 177 students, and the result is determined using tests that enable objective evaluation to prove the effectiveness of the program in helping students acquire the 'three sub-abilities' that constitute the abstraction ability.The educational programme proposed in this research, which focuses on the universality of the effects of learning drawing, as well as the quantitative criteria for evaluating it, will contribute to familiarize practical education in the field of art to the general public.
A DATA DRIVEN TOOL TO SUPPORT DESIGN TEAM COMPOSITION MEASURING SKILLS DIVERSITY
Authors: Chiarello Filippo , Spada Irene , Barandoni Simone , Giordano Vito , Fantoni Gualtiero 
Published: June 2023

AbstractTeam composition in Project Based Learning is the first task for the class and has a great impact on the learning experience. Anyway, little space is dedicated in literature about team composition, considering their personal inclinations towards design tasks.For these reasons we propose a tool that aims to map the design skills of students to optimise team composition. The tool is based on a questionnaire grounded in the design theory and aims at measuring the willingness of students at performing certain design tasks. The results of the questionnaires are analysed using Principal Component Analysis to normalise each students’ answers to the whole class, and to show the distribution of students in the space of engineering design skills.We present the design process of the tool, and a first experimentation on two classes of master's degree students in Management Engineering and Data Science, testing the tool on a total of 72 students. The results are promising and demonstrate the robusteness of the questionnaire and of the analytical method. Also, we propose next steps for our research activity, calling for other researchers to test our method in different contexts.
COMPARING ACADEMICS AND PRACTITIONERS Q & A TUTORING IN THE ENGINEERING DESIGN STUDIO
Authors: Hurst Ada , Lin Shirley , Treacy Claire , Nespoli Oscar G. , Gero John S. 
Published: June 2023

AbstractIn the design studio, academic (professor) and practitioner tutors provide individual mentoring to students as they progress in their design projects. Prior studies suggest that design practitioners may follow a different design process compared to academics, but little is known about how this difference relates to their design tutoring. This study explores the similarities and differences in tutoring by academics and practitioners. We use a question-asking lens to characterize the tutoring styles of four tutors - two academics and two practitioners - over a five-week design project in an engineering design studio. We find that academic tutors ask questions at a significantly higher rate than practitioner tutors, suggesting a more question-centred tutoring style. We also find that proportionally more of practitioner tutors’ questions are generative in nature, while the academic tutors employ more convergent thinking in their questioning. This may be an indicator of the practitioners' own design thinking, which might be more solution-focused than that of academics. These preliminary findings motivate future investigations of the relationship between differences in tutoring and impact on student design learning.
COMPLEX SYSTEMS DESIGN: SUSTAINABILITY CHALLENGES FOR SHIPBUILDING
Authors: Bouyssou Anne , Baumler Raphaël , Öhrwall Rönnbäck Anna 
Published: June 2023

AbstractShips are complex technical systems resulting from large scale and scope projects in which integration plays a key role, particularly because trade-offs have to be made between conflicting objectives.Merchant ships are usually built with a perspective of twenty-five years of service. Ship owners detail their requirements and ship specifications in line with their strategy to remain competitive on specific segments of the shipping markets. Ships serve and organize global trade flows. The rise in environmental regulations and technological changes generate unprecedented uncertainties for ship owners.Ships do not follow the usual systems engineering process, as there is no full-scale prototyping. Rules and standards deeply influence the design of ships and limit the possibilities to ’think outside the box’.The purpose of this paper is to present environmental drivers relating to the operation of the ship which have, or will have, an influence on the way it is designed.