bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensory and aroma impact of mitigation strategies against sunburn in Riesling
Authors: Szmania Caterina , Waber Jonas , Bogs Jochen , Fischer Ulrich 
Published: August 2023

Climate change is an environmental challenge that impacts the wine industry due to frequent sunburn damage triggered by heat waves, sun radiation and severe water deficits. This leads to severe yield losses and sensory changes in the resulting wines, presumably caused by climate-induced off-flavours. This study aims to develop viticultural and oenological strategies to mitigate sunburn damage in the highly sensitive variety Riesling and its detrimental sensory properties. In 2020, we combined the timing and intensity of defoliation measures with the application of kaolin and calcium hydroxide suspensions, reflecting a portion of the solar radiation. Seven treatments were replicated in three field experiments. Grapes from each field replication were maintained as a fermentation replication and, therefore, separately vinified following a standardised protocol. Replicates, including field and fermentation replicates, were further used as sensory replications. Descriptive analysis (DA) and temporal dominance of sensations (TDS) revealed a significant sensory impact of experimental trials in eight of twelve attributes. Additionally, wines were analysed by gas chromatography-mass spectrometry. The early sun exposition treatment with partial defoliation of the grape zone after flowering, followed by a second defoliation at berry closure, reduced fruity aroma but increased smoky notes due to enhanced 4-vinylguajacol formation as well as the intensity of the atypical ageing note (ATA) reminiscent of acacia blossom, naphthalene and fusel alcohols. Applying kaolin or calcium hydroxide particles on the berry skin slightly mitigated these unpleasant effects and even increased fruitiness and sweetness. Late defoliation at berry closure diminished the green note, which was most prominent in the non-defoliated control. Further smokiness and ATA intensities were lowered, in contrast to their rise due to early defoliation. However, late defoliation increased 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN), causing the petrol off-flavour. TDS analysis revealed a higher fruity and sweet dominance during the first 10 seconds due to early defoliation, while late defoliation fostered dominant and lingering sourness. In conclusion, early defoliation, which lowers sunburn incidence due to an early adaptation towards sun exposure, increases smoky and ATA flavours and diminishes fruitiness. These detrimental sensory effects could be significantly mitigated by applying reflecting particles of kaolin and calcium hydroxide on berry skins.
CONTRIBUIÇÕES DO PIBID-QUÍMICA DA UNIVERSIDADE FEDERAL DO NORTE DO TOCANTINS NA FORMAÇÃO DOCENTE
Authors: DA SILVA  MELO DONIZETE , Dos Santos Barbosa Brenda , Barbosa Gomes Verenna 
Published: August 2023

O trabalho buscou investigar as contribuições das ações do Programa Institucional de Bolsa de Iniciação à Docência - PIBID na formação dos licenciandos em Química. A pesquisa foi realizada com 6 bolsistas discentes do curso de Licenciatura em Química da Universidade Federal do Norte do Tocantins. Os dados foram coletados por meio da análise dos relatórios dos bolsistas. Ao analisar os seus relatos, foi possível identificar que a vivência dos alunos no campo de suas futuras atuações profissionais proporciona-lhes uma proximidade entre a teoria e a prática, o desenvolvimento das habilidades de leitura e escrita e a apropriação de metodologias de ensino que dialogam com as tendências pedagógicas atuais. De modo geral, os resultados sinalizam que a vivência dos licenciandos no PIBID tem promovido a consolidação de uma iniciação à docência prática e, sobretudo, reflexiva permitindo compreender alguns processos inerentes à formação docente.
Palavras-chave: PIBID-QUÍMICA, Formação docente, Universidade-Escola.
Precise surface functionalization of PLGA particles for human T cell modulation
Authors: Hadley Pierce , Chen Yuanzhou , Cline Lariana , Han Zhiyuan , Tang Qizhi , Huang Xiao , Desai Tejal 
Published: August 2023

Abstract
Biofunctionalization of synthetic materials has broad utility in various biomedical applications but was limited by insufficient efficiency and controllability for bioconjugation. Therefore, we developed a new platform of building synthetic DNA-scaffolds on material surfaces to assemble and organize functional cargos, allowing for more precise control over cargo density and ratio. The adaptation of this technology for biomedical applications requires diverse expertise ranging from materials to bioconjugation chemistry to cell biology, and there are many critical checkpoints to ensure the quality of the platform for the expected biological function. In this protocol, we describe the three key fabrication procedures involved: 1) fabrication of polymeric particles engrafted with DNA-scaffolds (3 days), 2) attachment of functional cargos with complementary DNA strands (3-4 days), and 3) surface assembly control and quantification (<1 day). We have also provided additional experimental design considerations for modifying the platform—for example, varying the material composition, size, or cargo types—which may be required for different biological needs. An area of increasing interest is immunomodulation, where immune cells have been recognized for their ability to sense extracellular cues to shape their phenotypic adaptation. We have reported how their modulation can be advanced by this precision biomaterial platform. Here, we exemplify the protocol of primary human T cells activation and identified various parameters that can impact T cell ex vivo manufacturing. This protocol will equip investigators with the necessary fabrication procedures and validation assays to design high-fidelity DNA-scaffolded biomaterials for uses in diverse biomedical applications.
Improving Spectral Efficiency of PSSCH for D2D Communication
Authors: singh Balwant , Tripathy malay Ranajan , Asthana Rishi , Vashishtha Jaya Nidhi 
Published: August 2023

Abstract
Improvement in the spectral efficiency (SE) for Device is Device (D2D) is important and essential to get enhancement in the data rate, bandwidth per unit area, the number of connected devices per unit area, availability, coverage, energy usage, and latency. This can be achieved by the implementation of a higher-order modulation technique. But the basic problem is interference. This paper is going to implement higher-order modulation (up to 1024 QAM) with the help of Transport Block Size (TBS) for the Physical Sidelink Shared Channel (PSSCH). Here SE is improving as the order of the modulation or TBS rises. Block error rate (BLER) and the Average number of transmissions are also cultivating as TBS or order of the modulation increasing. Development in the BLER reflects the enrichment in block error due to interference. Here frequency selective fading and additive white gaussian noise (AWGN) are added to the channel to see the effect of fading and noise.
IL-6 and IL-17 as potential links between pre-existing hypertension and long-COVID sequelae in patients undergoing hemodialysis: A multicenter cross-sectional study
Authors: Stepanova Natalia , Driianska Victoria , Rysyev Andriy , Ostapenko Tetyana , Kalinina Nataliia 
Published: August 2023

Abstract
Objective. We hypothesized that an increase in the production of interleukins (IL)-6 and IL-17 could serve as a potential mechanism linking pre-existing uncontrolled blood pressure (BP) to the occurrence of long-COVID sequelae in patients undergoing hemodialysis (HD).
Methods. In this cross-sectional study, we examined serum IL-6 and IL-17 levels in 80 patients undergoing HD, taking into account preinfection BP, the presence of long-COVID sequelae, and the time interval after acute COVID-19 infection, which was either 5 or 10 months. The cytokines were measured by ELISA assay. Controlled BP was defined as a 3-month average pre-dialysis BP <140/90 mmHg and post-dialysis <130/80 mmHg.
Results. The prevalence of long-COVID sequelae was significantly higher in patients with uncontrolled BP than in the BP-controlled group. Both IL-6 and IL-17 concentrations were also significantly higher in patients with uncontrolled BP compared with the BP-controlled group. The patients with long-COVID sequelae had higher IL-6 and IL-17 values than the fully recovered patients at both time points, but their concentrations decreased significantly over time.
Conclusions. Elevated levels of serum IL-6 and IL-17 in patients undergoing HD with pre-existing uncontrolled BP may be associated with developing long-COVID sequelae.
Insights into ZmWAKL in Maize Kernel Development: Genome-wide Investigation and GA-Mediated Transcription
Authors: Hu Kun , Dai Qiao , Ajayo Babatope , Wang Hao , Hu Yufeng , Li Yangping , Huang Huanhuan , Liu Hanmei , Liu Yinghong , Wang Yayun , Gao Lei , Xie Ying 
Published: August 2023

Abstract
Background
The functional roles of the WAK and WAKL kinase families in cellular expansion and developmental processes have been well-established. However, the molecular regulation of these kinases in maize development has been limited due to the absence of comprehensive genome-wide studies.
Results
In this investigation, we performed an in-depth analysis to identify 58 maize WAKL genes, classified into three distinct phylogenetic clusters. Moreover, through structural prediction analysis, we revealed functional conservation among WAKLs across maize. Promoter analysis uncovered the existence of cis-acting elements associated with the transcriptional regulation of ZmWAKL genes by Gibberellic acid (GA). To further elucidate the role of WAKL genes in maize kernels, we focused on three highly expressed genes, ZmWAKL38, ZmWAKL42 and ZmWAKL52, and conducted co-expression and expression analyses. Remarkably, their expression patterns exhibited a strong correlation with GA-responsive transcription factors TF5, TF6, and TF8, which displayed preferential expression in kernels. RT-qPCR analysis validated the upregulation of ZmWAKL38, ZmWAKL42, ZmWAKL52, TF5, TF6, and TF8 following GA treatment. Additionally, ZmWAKL52 promoter showed significant increase of transcriptional activity in the present of TF8, with ZmWAKL52 localized in both the plasma membrane and cell wall. TF5 positively regulated ZmWAKL38, while TF6 positively regulated ZmWAKL42.
Conclusions
Collectively, these findings provide novel insights into the characterization and regulatory mechanisms of specific ZmWAKL genes involved in maize kernel development, offering prospects for their utilization in maize breeding programs.
WITHDRAWN: Degradation of PET microplastic particles to monomers in human serum by PETase
Authors: Lopez-Lorenzo Ximena , Hueting David , Bosshard Eliott , Syrén Per-Olof 
Published: August 2023

Abstract
More than 8 bton of plastic waste has been generated posing severe environmental consequences and health risks. Due to prolonged exposure, microplastic particles are found in human blood and other bodily fluids. Despite a lack of toxicity studies regarding microplastics, harmful effects for humans seem plausible and cannot be excluded. As small plastic particles readily translocate from the gut to body fluids, enzyme-based treatment of serum could constitute a promising future avenue to clear synthetic polymers and their responding oligomers by their degradation into monomers of lower toxicity than the material they originate from. Still, whereas it is known that enzymatic depolymerization rate of synthetic polymers varies orders of magnitude depending on buffer and media composition, the activity of plastic degrading enzymes in serum was unknown. Here we report how an engineered PETase can expediently depolymerize crystalline microplastic-like particles of the commodity polymer polyethylene terephthalate (PET) into its non-toxic monomer terephthalic acid (TPA) alongside mono(2-hydroxyethyl) terephthalate (MHET) in human serum at 37°C. By developing an efficient method to depolymerize microplastics in vitro, our work takes a step closer to find a solution to the problem that microplastics in the bloodstream may pose in the future.
Clinical Significance of Circulating Tumor Cells in Metastatic Colorectal Cancer to the Peritoneum: A novel method for monitoring treatment response, and assessing minimal residual disease
Authors: Frühling Petter , Moberg Louice , Ghanipour Lana , Birgisson Helgi , Graf Wilhelm , Ericsson Christer , Cashin Peter 
Published: August 2023

Abstract
Cytoreductive surgery (CRS) (1), with or without hyperthermic intraperitoneal chemotherapy (HIPEC) (2), is the current standard treatment option for colorectal cancer with resectable peritoneal metastases (3) (4) (5). Systemic chemotherapy has often a limited response rate in colorectal peritoneal metastases leading to a controversy about its role in patients offered CRS (6) (7). Circulating tumor cells (CTCs) may constitute an important biomarker for monitoring disease progression, treatment response and residual disease in this setting. We prospectively followed 46 patients treated with CRS and HIPEC for colorectal cancer with peritoneal metastases and examined whether CTCs were present the week of surgery. The CTC measurements were made with the IsoPicTM technology, which is a platform technology used to detect CTCs based on the difference in biomechanical properties compared to blood resident cells. In 17 (37%) patients CTCs were detected. The presence of CTCs was associated with shorter recurrence free and overall survival after CRS and HIPEC. The main finding of our study was that both recurrence (HR 4.00, 95%CI 1.15-13.9; p=0.029) and overall survival (HR 5.91; 95% CI 1.18-29.7; p=0.03) were significantly worse if CTCs were detected after neoadjuvant treatment. Moreover, in the subgroup of patients with CTCs detected, adjuvant therapy tended to improve the prognosis while in CTC negative patients it did not appear to matter. Our results suggest that CTCs may constitute a dynamic personalized biomarker for prognostication, predicting response to therapy, and for monitoring disease progression in patients with colorectal cancer with metastases to the peritoneum. We anticipate that measuring CTCs dynamically before and after treatment, may contribute to a more personalized oncological therapy.
Database of Twitter Influencers in Cryptocurrency (2021-2023) with Sentiments
Authors: jahanbin kia , Chahooki Mohammed Ali Zare 
Published: August 2023

Abstract

Objectives: With the expansion of social networks such as Twitter, many experts share their opinions on various topics. The opinions of experts, who are also known as influencers, can be very influential. Combining these tweets and the historical prices of cryptocurrencies makes it possible to predict their price trends accurately. A Hybrid of RoBERTa deep neural network and BiGRU has been used for Sentiment Analysis (SA). Sentiments of tweets can be of great help to investors to understand the future behavior of the market and manage the stock portfolio. Unlike the tweets that are only extracted using the cryptocurrency name hashtag, the tweets of this dataset have specialized opinions and can determine the market trend.
Data description: The dataset created in this research concerns the opinions of more than 52 influencers (persons or companies) regarding eight cryptocurrencies. This dataset was collected through the Apify Twitter API from February 2021 to June 2023. This dataset contains five Excel files and tweets, compound score,importance coefficient of each tweet, sentiment polarity, and historical prices of our cryptocurrencies: Bitcoin, Ethereum, Binance, and other information. These tweets cover the opinions of 52 influencers on more than 300 cryptocurrencies,although most comments are related to Bitcoin, Ethereum, and Binance. For thisreason, three Excel files containing the historical prices of polarity and compoundsentiment related to Bitcoin, Ethereum, and Binance cryptocurrencies have beenplaced separately in the dataset. The polarity of sentiment in these Excel showsthe maximum number of polarities by applying the importance coefficient, whichdetermines the dominant polarity of sentiment related to a particular day for thecryptocurrency.
NK-92 cells labeled with Fe3O4-PEG-CD56/Avastin@Ce6 nanoprobes for the targeted treatment and noninvasive therapeutic evaluation of breast cancer
Authors: Lian Jingge , Duan Meng , Wang Zilin , Guo Xinyu , Ji Zaixiong , Gao Guo , Li Kangan 
Published: August 2023

Abstract
Adoptive cellular immunotherapy is critical for future clinical applications as a promising and alternative cancer therapy platform. Natural killer (NK) cells have attracted attention as an important type of innate immune regulatory cell that can rapidly kill multiple adjacent cancer cells. However, these cells are significantly less effective in treating solid tumors than in treating hematological tumors. Herein, we report the synthesis of a Fe3O4-PEG-CD56/Avastin@Ce6 nanoprobe labeled with NK-92 cells that can be used for adoptive cellular immunotherapy, photodynamic therapy and dual-modality imaging-based in vivo fate tracking. The labeled NK-92 cells specifically target the tumor cells, which increases the amount of cancer cell apoptosis in vitro. Furthermore, the in vivo results indicated that the labeled NK-92 cells can be used for tumor magnetic resonance imaging and fluorescence imaging, adoptive cellular immunotherapy, and photodynamic therapy after tail vein injection. These data show that the developed multifunctional nanostructure is a promising platform for efficient innate immunotherapy, photodynamic treatment and the noninvasive therapeutic evaluation of breast cancer.
The Rogdi Knockout Mouse is a Model for Kohlschütter–Tönz Syndrome
Authors: Jimenez-Armijo Alexandra , Morkmued Supawich , Ahumada José Tomás , Kharouf Naji , de Feraudy Yvan , Gogl Gergo , Riet Fabrice , Niederreither Karen , Laporte Jocelyn , Birling Marie Christine , Selloum Mohammed , Herault Yann , Hernandez Magali , Bloch-Zupan Agnès 
Published: August 2023

Abstract
Kohlschütter–Tönz syndrome (KTS) is a rare autosomal recessive disorder characterized by severe intellectual disability, early-onset epileptic seizures, and amelogenesis imperfecta. Loss of ROGDI expression likely produces these severe patient defects. Here, we present a novel Rogdi mutant mouse demonstrating that Rogdi-/- loss of function recapitulates most KTS patient symptoms. Mutants displayed pronounced pentylenetetrazol-induced seizures, confirming epilepsy susceptibility. Spontaneous locomotion and circadian activity tests demonstrate Rogdi mutant hyperactivity mirroring patient spasticity. Object recognition impairment indicates memory deficits. Rogdi-/- mutant enamel was markedly less mature. Scanning electron microscopy confirmed its hypomineralized/hypomature crystallization, as well as its low mineral content. Transcriptomic RNA sequencing of postnatal day 5 lower incisors showed downregulated enamel matrix proteins Enam, Amelx, and Ambn. Enamel crystallization is highly pH-dependent, as an acidic pH is required to accelerate matrix protein degradation and to promote mineralization. Rogdi-/- teeth exhibit no signs of cyclic dental acidification. Additionally, expression changes in Wdr72, Slc9a3r2, and Atp6v0c were identified as potential contributors to these tooth acidification abnormalities. These proteins interact through the acidifying V-ATPase complex. Here, we present the Rogdi-/- mutant as a novel model to partially decipher KTS pathophysiology. Rogdi-/- mutant defects in acidification might explain the unusual combination of enamel and rare neurological disease symptoms.
Entropy creation, Waste work and Thermodynamic Efficiency of Galvanic Cells: Effects of Discharge Current and Environment Temperature
Authors: Guha Sirshendu 
Published: August 2023

Abstract
In this work, knowledge of efficiency from the perspective of second law for chemical processes (Guha
S. Environmental Progress and Sustainable Energy, 39(2), 2020; Guha S., International Journal of Industrial Chemistry, 11(2), 2020,) has been applied to find out how environment conditions and discharge current affect critical performance parameters such as Waste Work, Thermodynamic Efficiency and irreversible Entropy Creation of any Galvanic Cell. Calculations are carried out for Galvanic Cell discharge operation to evaluate magnitude of irreversible Entropy Creation, Waste Work and Thermodynamic Efficiency using second law efficiency as a basis rather than mere energy balance concept.
It is found that irreversible Entropy Creation, Waste Work and Thermodynamic Efficiency are strongly dependent on environment temperature and discharge current.
Finally, for maximizing electrical energy output, a Galvanic Cell with exothermic discharge reaction should be operated at lower discharge current and at a cell operating temperature which is close to the environment temperature ensuring minimum difference between these two temperature values.
Similarly, a Galvanic Cell with endothermic discharge reaction should also be operated at lower discharge current and at cell operating temperature which is close to the environment temperature maintaining minimum difference between these two temperatures for maximizing electrical energy output.
Lower cell discharge current and minimum difference between the cell and environment temperatures will ensure higher Availability which in turn will lead to higher Thermodynamic Efficiency.
Structural and Thermodynamic Insights into Antibody Light Chain Tetramer Formation through 3D Domain Swapping
Authors: Hirota Shun , Sakai Takahiro , Mashima Tsuyoshi , Kobayashi Naoya , Ogata Hideaki , Duan Lian , Fujiki Ryo , Hengphasatporn Kowit , Uda Taizo , Shigeta Yasuteru , Hifumi Emi 
Published: August 2023

Abstract
Monoclonal antibodies are successfully used in the fields of pharmacy and medicine. While antibodies easily form aggregates and may lose the ability to recognize antigens, atomic-level structural examinations of antibody light chain aggregates are sparse. In this study, we present an antibody light chain that maintains an equilibrium between its monomeric and tetrameric states. According to data from X-ray crystallography, thermodynamic and kinetic measurements, as well as theoretical studies, this antibody light chain engages in 3D domain swapping within its variable region. Here, a pair of domain-swapped dimers creates a tetramer through hydrophobic interactions, facilitating the revelation of the domain-swapped structure. The negative cotton effect linked to the β-sheet structure, observed around 215 nm in the circular dichroism spectrum of the tetrameric variable region, is more pronounced than that of the monomer. This suggests that the monomer contains less β-sheet structures and exhibits greater flexibility than the tetramer in solution. These findings not only clarify the domain-swapped structure of an antibody light chain but also contribute to controlling antibody quality and advancing the development of future molecular recognition agents and drugs.