bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective Association of APOC1/rs4420638 with Risk of Obesity: A case-control Study in Portuguese Children
Authors: Manco Licínio , Albuquerque David , Rodrigues Daniela , Machado-Rodrigues Aristides M. , Padez Cristina 
Published: June 2023

AbstractThe association of the rs4420638 polymorphism, near the APOC1 gene, was examined with the risk of obesity among Portuguese children. A sample of 446 Portuguese individuals (231 boys and 215 girls) of European descent, aged 3.2 to 13.7 years old (mean age: 7.98 years), were selected to conduct a case-control study. Body mass index (BMI), BMI Z-scores, and waist circumference were calculated. Genotyping was performed by real time PCR using a pre-designed TaqMan probe. Logistic regression and the nonparametric Mann-Whitney test were used to test the associations. The association results revealed a significant protective effect from the minor G-allele of SNP rs4420638 against obesity, with an odds ratio (OR) of 0.619 (95% CI 0.421–0.913; p = 0.0155) in the additive model, and OR of 0.587 (95% CI 0.383-0.9; p = 0.0145) in the dominant model. Moreover, comparing genotype groups (AA vs. AG + GG), significantly lower values (p < 0.05) for the anthropometric traits weight, height, BMI, BMI Z-score and waist circumference, were observed in the carriers of allele G. The present study provides further evidence for the APOE/APOC1 candidate-region association with the risk of obesity. This was the first study to describe the protective association of the rs4420638 minor G-allele against obesity in childhood exclusively.
Affective Atmosphere in an Art Fair Jewel Heist
Authors: Yates Donna , Bērziņa Diāna 
Published: June 2023

AbstractIn mid 2022, social media videos of an armed jewel heist during opening hours at the TEFAF art fair baffled the public. The thieves wore seemingly absurd costumes, and the art fair attendees only showed muted reactions to a violent and dramatic crime. Drawing on first-hand observational research at TEFAF before and after the heist, and on an extended observational methodology focused on the atmosphere of art commercial spaces, we argue that the affective atmosphere of the art fair had a direct influence both on how the thieves committed and how onlookers experienced crime. Within the art fair space, thieves conformed to art world conventions and fair attendees experienced dangerous events as being significantly less sinister. With this paper, we contribute to the growing concept of sensory criminology and the role that atmosphere and affect play in crime.
Deep learning for opinion mining and topic classification of course reviews
Authors: Koufakou Anna 
Published: June 2023

AbstractStudent opinions for a course are important to educators and administrators, regardless of the type of the course or the institution. Reading and manually analyzing open-ended feedback becomes infeasible for massive volumes of comments at institution level or online forums. In this paper, we collected and pre-processed a large number of course reviews publicly available online. We applied machine learning techniques with the goal to gain insight into student sentiments and topics. Specifically, we utilized current Natural Language Processing (NLP) techniques, such as word embeddings and deep neural networks, and state-of-the-art BERT (Bidirectional Encoder Representations from Transformers), RoBERTa (Robustly optimized BERT approach) and XLNet (Generalized Auto-regression Pre-training). We performed extensive experimentation to compare these techniques versus traditional approaches. This comparative study demonstrates how to apply modern machine learning approaches for sentiment polarity extraction and topic-based classification utilizing course feedback. For sentiment polarity, the top model was RoBERTa with 95.5% accuracy and 84.7% F1-macro, while for topic classification, an SVM (Support Vector Machine) was the top classifier with 79.8% accuracy and 80.6% F1-macro. We also provided an in-depth exploration of the effect of certain hyperparameters on the model performance and discussed our observations. These findings can be used by institutions and course providers as a guide for analyzing their own course feedback using NLP models towards self-evaluation and improvement.
Cost effectiveness, nitrogen, and phosphorus removal in field-based woodchip bioreactors treating agricultural drainage water
Authors: Plauborg Finn , Skjødt Maja H. , Audet Joachim , Hoffmann Carl C. , Jacobsen Brian H. 
Published: June 2023

AbstractNitrogen (N) and phosphorus (P) losses to surface and coastal waters are still critically high across Europe and globally. Measures to mitigate and reduce these losses are being implemented both at the cultivated land surface and at the edge-of-fields. Woodchip bioreactors represent a new alternative in Denmark for treating agricultural drainage water, and the present study—based on two years of data from five Danish field-based bioreactors—determined N removal rates varying from 1.49 to 5.37 g N m−3 d−1 and a mean across all bioreactors and years of 2.90 g N m−3 d−1. The loss of phosphorus was relatively high the first year after bioreactor establishment with rates varying from 298.4 to 890.8 mg P m−3 d−1, but in the second year, the rates ranged from 12.2 to 77.2 mg P m−3 d−1. The investments and the costs of the bioreactors were larger than expected based on Danish standard investments. The cost efficiency analysis found the key issues to be the need for larger investments in the bioreactor itself combined with higher advisory costs. For the four woodchip bioreactors considered in the cost efficiency analysis, the N removal cost was around DKK 350 per kg N ($50 per kg N), which is ca. 50% higher than the standard costs defined by the Danish authorities. Based on the estimated costs of the four bioreactor facilities included in this analysis, a bioreactor is one of the most expensive nitrogen reduction measures compared to other mitigation tools.
New microalgae media formulated with completely recycled phosphorus originating from agricultural sidestreams
Authors: Beyer Albert S. , Meier Jasmin , Jiménez-Muñoz Marina , Meixner Raphael , Ende Stephan S. W. , Abomohra Abdelfatah , Henjes Joachim 
Published: June 2023

AbstractThis study investigated the impact of struvite as a sustainable phosphorus source on the growth and phycocyanin production by the blue-green alga Arthrospira platensis. Three modified growth media were compared to the typical SAG-spirul culture media. CS(+) refers to the completely recycled struvite from bovine urine as a phosphate source, while S(-) and S(+) refer to commercially available struvite as a phosphate source. On media with (+), a pre-treatment was conducted to evaporate NH4, as it negatively affects cell growth and functions of the photosynthetic apparatus at high concentrations, and to release phosphate due to the low solubility of struvite in water. For each medium, three cultures were cultivated in Erlenmeyer flasks for a duration of 42 days. Results showed that no statistically significant negative effect of struvite was found on the growth rates. However, C-phycocyanin (CPC-P) in CS(+) and S(+) was significantly higher compared to CPC-P in untreated growth media. The study hypothesized that low concentrations of NH3 remaining after the pre-treatment of struvite could have a positive impact on phycocyanin accumulation, as an energy efficient and quick nitrogen source for A. platensis.
Use of iRNA in the post-transcriptional gene silencing of necrosis-inducing Phytophthora protein 1(NPP1) in Phytophthora cinnamomi
Authors: Pascoal-Ferreira Patrick , Chahed Abdessalem , Costa Rodrigo , Branco Iuliia , Choupina Altino 
Published: June 2023

Abstract
Background
Phytophthora cinnamomi is an Oomycetes associated with soil, this Oomycete is one of the most destructive species of Phytophthora, being responsible for the decline of more than 5000 ornamental, forest, or fruit plants. It can secrete a class of protein NPP1 (Phytophthora necrosis inducing protein 1), responsible for inducing necrosis in leaves and roots of plants, leading to their death.

Objective
This work will report the characterization of the Phytophthora cinnamomi NPP1 gene responsible for the infection of Castaneasativa roots and will characterize the mechanisms of interaction between Phytophthora cinnamomi and Castanea sativa, by gene silencing NPP1 from Phytophthora cinnamomi mediated by RNAi.

Methods and results
For silencing a part of the coding region of the NPP1 gene, was placed in the sense and antisense directions between an intron and ligated to the integrative vector pTH210. Cassette integration was confirmed by PCR and sequencing on the hygromycin-resistant Phytophthora cinnamomi transformants. Transformants obtained with the silenced gene was used to infect Castanea sativa.

Conclusions
Plants infected with these transformants showed a great reduction in disease symptoms, confirming iRNA as a potential alternative biological tool in the study of molecular factors, and in the control and management of Phytophthora cinnamomi.
The structural components of the Azotobacter vinelandii iron-only nitrogenase, AnfDKG, form a protein complex within the plant mitochondrial matrix
Authors: Johnston E. , Okada S. , Gregg C. M. , Warden A. C. , Rolland V. , Gillespie V. , Byrne K. , Colgrave M. L. , Eamens A. L. , Allen R. S. , Wood C. C. 
Published: June 2023

AbstractA long-held goal of synthetic biology has been the transfer of a bacterial nitrogen-fixation pathway into plants to reduce the use of chemical fertiliser on crops such as rice, wheat and maize. There are three classes of bacterial nitrogenase, named after their metal requirements, containing either a MoFe-, VFe- or FeFe-cofactor, that converts N2 gas to ammonia. Relative to the Mo-nitrogenase the Fe-nitrogenase is not as efficient for catalysis but has less complex genetic and metallocluster requirements, features that may be preferable for engineering into crops. Here we report the successful targeting of bacterial Fe-nitrogenase proteins, AnfD, AnfK, AnfG and AnfH, to plant mitochondria. When expressed as a single protein AnfD was mostly insoluble in plant mitochondria, but coexpression of AnfD with AnfK improved its solubility. Using affinity-based purification of mitochondrially expressed AnfK or AnfG we were able to demonstrate a strong interaction of AnfD with AnfK and a weaker interaction of AnfG with AnfDK. This work establishes that the structural components of the Fe-nitrogenase can be engineered into plant mitochondria and form a complex, which will be a requirement for function. This report outlines the first use of Fe-nitrogenase proteins within a plant as a preliminary step towards engineering an alternative nitrogenase into crops.
The Personal is Political: Internalized Sexual Stigma and the Desire for Gender Complementarity in (some) Same-Gender Relationships
Authors: Napier Jaime L. , van der Toorn Jojanneke , Vial Andrea C. 
Published: June 2023

AbstractWe examined whether gay men (Studies 1–2) and lesbian women (Study 1) who harbor internalized stigma due to their sexuality will desire a romantic relationship that reflects conventional, complementary gender roles where one partner is stereotypically feminine and the other is stereotypically masculine, in terms of both personality traits and division of household labor. Results showed that, among gay men with high (but not low) internalized stigma, self-ascribed masculinity was positively related to preferences for an ideal partner with stereotypically feminine traits. Preferences for partners with gender complementary traits did not emerge among women, or among men high in self-ascribed femininity. Contrary to predictions, internalized stigma was not associated with preferences for a gender-complementary division of household chores. Instead, internalized stigma was associated with the avoidance of tasks that are stereotypically gender incongruent—women high (vs. low) in stigma preferred for the partner (vs. self) to do so-called masculine (but not feminine) chores, whereas men high (vs. low) in stigma preferred for the partner (vs. self) to do stereotypically feminine (but not masculine) chores. Study 2 also included an experimental manipulation to test whether these effects were influenced by societal exclusion or acceptance, but there was no evidence of this.
University Research Centres, Scientific Freedom, and the Jester’s Paradox
Authors: Noe Egon Bjørnshave , Alrøe Hugo F. 
Published: June 2023

AbstractThe key norm of good science is research integrity, which includes the freedom to inquire as an independent, self-organising system, and the responsibility to identify, frame, and engage in the problems of society, in a scientific manner. This paper investigates the challenges to scientific integrity experienced by university research centres. Research centres are organised around specific problematic fields in society and are expected to have specific societal impacts. Therefore, they are born with the paradox of being restricted in terms of scientific freedom yet required to meet science standards. As an example, we analyse the Danish Centre for Rural Research (CLF) which, like many other institutions of science and research centres, has become increasingly dependent on various external funding over the past decades. In social systems theoretical terms, research centres are hybrid organisations that operate simultaneously in the function systems of science, politics, and economy. The question is whether it is possible for research centres to uphold the requisite research integrity to provide society with truthful and critical knowledge – i.e. to uphold the necessary autopoiesis of the science function system, operating in the medium of truth – and at the same time be able to navigate in the structures of power that the centre is faced with, in terms of funding, outside control, and expectations of expectations. The medieval court jester, who was able to speak unwelcome truths to the all-mighty king without getting his head cut off, was a solution to this kind of paradox. The question is how we can handle this paradox in contemporary sciences, increasingly depending on external funding.
Factors influencing somatic cell counts and bacterial contamination in unpasteurized milk obtained from water buffalo in Bangladesh
Authors: Singha Shuvo , Ceciliani Fabrizio , Rahman Md. Mizanur , Mannan Mohammad Abdul , Chowdhury Salma , Nath Sanjib Chandra , Paul Ovirup Bhushan , Persson Ylva , Boqvist Sofia 
Published: June 2023

AbstractLittle has been published on the factors influencing the safety and quality of milk derived from water buffalo in Bangladesh. This study aims to describe the milk hygiene parameters and milk chain characteristics of unpasteurized raw milk sold to consumers in order to improve milk hygiene. A quantitative study design evaluated somatic cell counts, total bacterial counts, and specific gram-negative (Enterobacteria) and gram-positive (staphylococci) pathogens in 377 aseptically collected milk samples. Samples were collected at multiple nodes along the buffalo milk value chain: 122 bulk tank milk samples were collected at the farm level, 109 milk samples at the middlemen level, and 111 milk samples at the milk collection centers. In addition, 35 samples were taken from various milk products at the retail level. It was found that progressively increasing somatic cell counts and bacterial counts, including potential pathogens, occurred along the milk chain. A seasonal increase in spring was found, varying based on the farming system (semi-intensive versus intensive). Other factors included water purity and cleanliness of containers, mixing buffalo and cow’s milk, and the location of the water buffalo milk producer (coastal or river basin). This study demonstrated how improving udder health and milk hygiene along the water buffalo milk value chain would increase the safety and quality of water buffalo milk in the study area.
Essential oils and extracts of plants as biocides against microorganisms isolated from the ruins of the Roman city of Conímbriga (Portugal)
Authors: Mateus Dina M. R. , Ferraz Eduardo , Perna Vera , Sales Pedro , Hipólito-Correia Virgílio 
Published: June 2023

AbstractBiodeterioration of monumental complexes is in large part due to the proliferation of various microorganisms that attack the physical–chemical structures of support materials. Various conservation and restoration interventions use commercial biocides of synthetic origin, which exhibit some human and environmental toxicity and sometimes side effects on support materials. The main objective of this work is the assessment of new biocides obtained from endemic Mediterranean plants, to be used in the preservation of cultural heritage with the goal of contributing to the sustainable use of ecosystems and to the development of Mediterranean local communities. The biocidal potential of essential oils (EOs) and solvent extracts (SEs) (ethanol and n-hexane) obtained from four plants were evaluated: Thymus mastichina (Tm), Mentha pulegium (Mp), Foeniculum vulgare (Fv), and Lavandula viridis (Lv). Microorganisms collected at an emblematic site of Portuguese cultural heritage, the ruins of the Roman city of Conímbriga, were used to evaluate the biocidal activity of the EOs and SEs. It can be concluded that (i) SEs did not exhibit fungicidal nor bactericidal activity, except for one fungus specie; (ii) biocidal activity of EOs depends on the microorganism specie. The EOs showed a relative average biocidal activity (when compared to the commercial biocide Biotin T (1% v/v)) of 64%, 32%, 30%, and 25% for Mp, Fv, Lv, and Tm. On carbonate rocks, the application of Fv and Mp EOs up to 3 layers do not promote significant color/tonality changes in the surface of the rock. And the application of three layers of Lv and four layers of Fv, Mp, and Lv OEs only promote the occurrence of blurs or stains (variation of tonality) on rocks that presents very low porosity. It can also be noted that the EO of Mp has the broadest spectrum of activity. The results allow considering the use of Mp, Fv, Lv, and Tm EOs as valid alternatives to commercial biocides, providing a prospective application in the field of green conservation of building heritage.
Radiomics applications in cardiac imaging: a comprehensive review
Authors: Polidori Tiziano , De Santis Domenico , Rucci Carlotta , Tremamunno Giuseppe , Piccinni Giulia , Pugliese Luca , Zerunian Marta , Guido Gisella , Pucciarelli Francesco , Bracci Benedetta , Polici Michela , Laghi Andrea , Caruso Damiano 
Published: June 2023

AbstractRadiomics is a new emerging field that includes extraction of metrics and quantification of so-called radiomic features from medical images. The growing importance of radiomics applied to oncology in improving diagnosis, cancer staging and grading, and improved personalized treatment, has been well established; yet, this new analysis technique has still few applications in cardiovascular imaging. Several studies have shown promising results describing how radiomics principles could improve the diagnostic accuracy of coronary computed tomography angiography (CCTA) and magnetic resonance imaging (MRI) in diagnosis, risk stratification, and follow-up of patients with coronary heart disease (CAD), ischemic heart disease (IHD), hypertrophic cardiomyopathy (HCM), hypertensive heart disease (HHD), and many other cardiovascular diseases. Such quantitative approach could be useful to overcome the main limitations of CCTA and MRI in the evaluation of cardiovascular diseases, such as readers’ subjectiveness and lack of repeatability. Moreover, this new discipline could potentially overcome some technical problems, namely the need of contrast administration or invasive examinations. Despite such advantages, radiomics is still not applied in clinical routine, due to lack of standardized parameters acquisition, inconsistent radiomic methods, lack of external validation, and different knowledge and experience among the readers. The purpose of this manuscript is to provide a recent update on the status of radiomics clinical applications in cardiovascular imaging.
Epigenetic Connections of the TRPA1 Ion Channel in Pain Transmission and Neurogenic Inflammation — a Therapeutic Perspective in Migraine?
Authors: Fila Michal , Pawlowska Elzbieta , Szczepanska Joanna , Blasiak Janusz 
Published: June 2023

AbstractPersistent reprogramming of epigenetic pattern leads to changes in gene expression observed in many neurological disorders. Transient receptor potential cation channel subfamily A member 1 (TRPA1), a member of the TRP channels superfamily, is activated by many migraine triggers and expressed in trigeminal neurons and brain regions that are important in migraine pathogenesis. TRP channels change noxious stimuli into pain signals with the involvement of epigenetic regulation. The expression of the TRPA1 encoding gene, TRPA1, is modulated in pain-related syndromes by epigenetic alterations, including DNA methylation, histone modifications, and effects of non-coding RNAs: micro RNAs (miRNAs), long non-coding RNAs, and circular RNAs. TRPA1 may change epigenetic profile of many pain-related genes as it may modify enzymes responsible for epigenetic modifications and expression of non-coding RNAs. TRPA1 may induce the release of calcitonin gene related peptide (CGRP), from trigeminal neurons and dural tissue. Therefore, epigenetic regulation of TRPA1 may play a role in efficacy and safety of anti-migraine therapies targeting TRP channels and CGRP. TRPA1 is also involved in neurogenic inflammation, important in migraine pathogenesis. The fundamental role of TRPA1 in inflammatory pain transmission may be epigenetically regulated. In conclusion, epigenetic connections of TRPA1 may play a role in efficacy and safety of anti-migraine therapy targeting TRP channels or CGRP and they should be further explored for efficient and safe antimigraine treatment. This narrative/perspective review presents information on the structure and functions of TRPA1 as well as role of its epigenetic connections in pain transmission and potential in migraine therapy.
Repressor Element-1 Binding Transcription Factor (REST) as a Possible Epigenetic Regulator of Neurodegeneration and MicroRNA-Based Therapeutic Strategies
Authors: Nassar Ajmal , Satarker Sairaj , Gurram Prasada Chowdari , Upadhya Dinesh , Fayaz SM , Nampoothiri Madhavan 
Published: June 2023

AbstractNeurodegenerative disorders (NDD) have grabbed significant scientific consideration due to their fast increase in prevalence worldwide. The specific pathophysiology of the disease and the amazing changes in the brain that take place as it advances are still the top issues of contemporary research. Transcription factors play a decisive role in integrating various signal transduction pathways to ensure homeostasis. Disruptions in the regulation of transcription can result in various pathologies, including NDD. Numerous microRNAs and epigenetic transcription factors have emerged as candidates for determining the precise etiology of NDD. Consequently, understanding by what means transcription factors are regulated and how the deregulation of transcription factors contributes to neurological dysfunction is important to the therapeutic targeting of pathways that they modulate. RE1-silencing transcription factor (REST) also named neuron-restrictive silencer factor (NRSF) has been studied in the pathophysiology of NDD. REST was realized to be a part of a neuroprotective element with the ability to be tuned and influenced by numerous microRNAs, such as microRNAs 124, 132, and 9 implicated in NDD. This article looks at the role of REST and the influence of various microRNAs in controlling REST function in the progression of Alzheimer’s disease (AD), Parkinson’s disease (PD), and Huntington’s disease (HD) disease. Furthermore, to therapeutically exploit the possibility of targeting various microRNAs, we bring forth an overview of drug-delivery systems to modulate the microRNAs regulating REST in NDD.
Graphical abstract