bluuebunny/crossref_metadata_2025_split huggingface dataset:
Network biology of lapatinib resistance in different types of HER2-positive breast cancers informs the prioritization of the combination anticancer treatments as anti-resistance interventions
Authors: Alkhawaja Bayan , Khaleel Anas , Abu Dayyih Wael , Hamad Mohammed F. , Hajjo Rima , Hailat Mohammad 
Published: March 2023

Background: With widespread therapeutic advancement, targeted anticancer therapeutics are taking over traditional treatment protocols. Nevertheless, the resistance to targeted therapeutics has halted the enthusiastic treatment response. An example of targeted therapy is Lapatinib. Lapatinib is a tyrosine kinase inhibitor used in HER2-positive breast cancer. It is widely used in HER2-positive metastatic breast cancer in combination with other drugs. Methods: This study's main objective was to provide a plausible mechanistic insight into lapatinib’s resistance in two HER2 positive breast cancer cell lines, SKBR3 and BT-474. We performed gene set analysis to identify the differentially expressed genes (DEG) in response to treatment with lapatinib from the gene expression profiles obtained from GSE38376 and GSE16179. The DEG was then analyzed by Ingenuity Pathway Analysis (IPA). Results: The IPA analysis showed that the increased expression of Hypoxia-inducible factor-1 alpha (HIF-1α) and Wnt/β-catenin and their related networks were associated with resistance and poor prognosis in SKBR3 and BT-474 cell lines, respectively. Although both cell lines are categorized as HER2 positive cell lines and in some reports were used interchangeably, in our hands, the two cell lines exhibited different biological pathways underlying resistance to lapatinib. In addition, among the other top canonical pathways, TNF was identified as the top upstream regulator in SKBR3 cell lines, whereas the Microphthalmia-associated transcription factor (MITF) was predicted as a top regulator in BT-474 cell lines. Conclusions: This study highlights the relevance of HIF-1α and Wnt/β-catenin compensatory networks in resistance toward lapatinib. Our findings outline the activation of angiogenesis and invasion processes in resistant cells with differential underlining gene networks in two different HER2 positive cell lines. The two cell lines reflect two different types of breast cancer, and hence the treatment strategy to avoid resistance should be planned differently.
A feasibility study of training in a local community aimed upon health promotion with special emphasis on musculoskeletal health effects
Authors: Prawiradilaga Rizky Suganda , Bendtsen Magnus , Esrup Simon , Jørgensen Niklas Rye , Yulianto Fajar Awalia , Helge Eva Wulff 
Published: March 2023

Background: To minimize fracture risk, multimodal training regimens are recommended. However, their effectiveness in community settings remains uncertain. This study evaluated the feasibility of 19-weeks of multimodal training in a local community center with emphasis on musculoskeletal health in postmenopausal women. Methods: In a controlled trial, 28 postmenopausal women (53-68-years-old) were assigned to a multimodal training group (MMT, n=15) or a control group (CON, n=13). The training consisted of high- and odd-impact, resistance and balance-coordination training 1-2 hours weekly. The outcomes were attendance rate, regional and total bone mineral density (BMD), bone mineral content (BMC), bone turnover markers (BTM), body composition, functional muscle strength and power, and dynamic balance. All were determined at baseline and after 19 weeks of training. BTM was assessed after three weeks. Results: Overall, 22(79%) participants (MMT, n=9; CON, n=13) completed the study, and the mean attendance rate for MMT was 65.5% of the maximum sessions (2) offered. Only right trochanter BMD increased (p<0.05) by 1.0±1.1% in MMT, which was higher(p<0.05) than CON. While whole-body BMC was not changed at 19 weeks from baseline in MMT, it decreased (p<0.05) in CON resulting in a significant difference (p<0.05) in whole-body BMC delta values between the two groups. Compared to baseline, body fat percentage(%BF), fat mass(FM), and visceral adipose tissue (VAT)-mass and -volume were decreased (p<0.01) in MMT, and were larger (p<0.05) than CON. No significant changes were observed in BTM, muscle strength and power, and dynamic balance after 19 weeks. Conclusions: Nineteen weeks of multimodal training 1-2 hours per week in a local community had a health-enhancing effect on %BF, FM, and VAT, whereas the musculoskeletal health impact was modest. We hypothesize that the reason might be too low training volume and frequency and supposedly too low musculoskeletal training intensity for some participants. Registration: ClinicalTrials.gov NCT05164679 (21/12/2021).
ISOLATION OF METHANOTROPHIC BACTERIA FROM NATURAL BIOCENOSES AND SELECTION OF NUTRIENT MEDIA OPTIMAL FOR CULTIVATION IN LABORATORY CONDITIONS
Authors: Prokudina Olga V. , Pestsov Georgiy V. , Tretyakova Anastasia V. 
Published: March 2023

Background. One of the most pressing problems in the world is the production of food protein. The global protein deficit is estimated at 30 million tons per year, for the Russian Federation it is estimated at 2-2.5 million tons of protein per year.
The problem of protein deficit can be partially solved with the help of methanotrophic bacteria. Under optimal conditions, methanotrophic bacteria are able to oxidise actively natural gas – methane and increase their biomass, rich in valuable protein, vitamins and other biologically active substances. The feed product from methanotrophic bacteria is comparable to fish meal and soybean meal, the concentration of pure protein in it is more than 80%.
Purpose. Isolation of methanotrophic bacteria from natural biocenoses and their cultivation on various nutrient media in laboratory conditions.
Materials and Methods. The study was conducted from 2019 to 2022 in the microbiological laboratory of the Center for Technological Excellence “Advanced Chemical and Biotechnology” of the Tolstoy Tula State Pedagogical University. The object of the study was methanotrophic bacteria isolated from various natural and artificial ecosystems, collection strains of Methylococcus capsulatus B-2990 and Methylomonas methanica B-2110. To isolate methanotrophic bacteria, the method of seeding a accumulative culture on a solid agiarized sterile nutrient medium in Petri dishes was used. For the cultivation of methanotrophs, nutrient media of various compositions containing all the necessary macro- and microelements for the normal growth of bacterial colonies were used.
Results. Colonies of methanotrophic bacteria were found in one sample of bottom sediments taken from the sphagnum swamp of the village of Kochaki in the Tula region and in samples from a methane reactor. As a result of comparison of nutrient media for isolation and cultivation of methanotrophs, it was found that the most suitable medium for isolation of methanotrophic bacteria is the standard nitrate mineral medium NMS. For the cultivation of methanotrophic bacteria, the best media were: the nutrient medium with the addition of anaerobic sludge, the ammonium mineral nutrient medium (AMS), and AMS with addition of methanol, the nutrient medium No.221 with addition of methanol.
Conclusions. The use of various nutrient media for the cultivation of methanotrophic bacteria makes it possible to assess the effect of various organic substances on the growth and development of bacterial colonies. The study of various natural and artificial ecosystems makes it possible to expand taxonomic knowledge about this group of microorganisms, as well as to increase the potential of using methanotrophs in solving various problems of ecology and biotechnology.
THE ISSUES OF CHARACTER ACCENTUATION ADOLESCENTS WITH DIABETES
Authors: Kolkova Svetlana M. , Zhivaeva Yulia V. , Stoyanova Ekaterina I. , Kazakova Tatiana V. , Kembel Vera R. , Guseva Tatiana A. 
Published: March 2023

Purpose. Theoretical justification and empirical study of the characteristics of adolescents’ personality with diabetes mellitus
Method. The study was conducted on a sample of 40 adolescents (20 with diabetes and 20 without). To determine the character accentuations, the following were used: Leonhard’s questionnaire and the method of identification characterological verbal portraits – E.G. Eidemiller. To compare the groups, correlation analysis was used (Mann-Whitney U test).
Results. Within group of adolescents with diabetes, the sensitive (40%) and schizoid (30%) types of accentuation prevail, while in healthy adolescents, the hyperthymic (55%) type of accentuation prevails. Adolescents with diabetes were more conflicted and less capable of self-control than a group of adolescents without a diagnosis. On average, accentuations were more pronounced in healthy adolescents, the average scores for each of the accentuations in patients with diabetes mellitus were lower. Also, the correlation coefficients between the indicators of the K. Leonhard method and the indicators of the E.G. Eidemiller method on samples of adolescents with and without a diagnosis of diabetes mellitus turned out to be different. In the group of adolescents with diabetes mellitus, correlations were observed between the following parameters: “emotive type” and “neurasthenic type” (r=0.81, p=0.05), “anxious type” and “paranoid type” (r=0.65, p=0.05), “excitable type” and “unstable type” (r=0.65, p=0.05). In a group of healthy adolescents, the following correlations were found between the indicators of the methods of K. Leonhard and E. G. Eidemiller: “pedantic type” and “hyperthymic type” (r=0.63, p=0.05), “exalted type” and “paranoid type” (r=0.65, p=0.05), “pedantic type” and “hysterical type” (r=0.70, p=0.05), “conformal type” (r=0.63, p=0.05), “cyclothymic type” and “conformal type” (r=0.67, p=0.05).
Conclusion. Such results will allow psychologists to more accurately take into account the personal characteristics of adolescents with diabetes and provide them with more effective assistance. Targets for work with adolescent with diabetes mellitus can be their introversion and, accordingly, assiste them in social adaptation, help with lack of self-control, aggressiveness.
EVOLUTION OF MASSAGE AS ONE OF THE DIRECTIONS OF MANUAL MEDICINE IN RUSSIA
Authors: Didur Mihail D. , Egorova Irina A. , Novoseltsev Svyatoslav V. , Zinkevich Elena R. 
Published: March 2023

Background. The article presents the evolution of massage in Russia - one of the directions of manual medicine. The study of such an evolutionary process is due to the need to comprehend the mental, historical and methodological foundations of massage, allowing to assess the importance of this direction for the treatment of patients. The content of the article shows the attitude of people to massage in Ancient Russia, illustrates its progressive development from the beginning of the XVI to the end of the XVIII centuries, describes the milestones of the development of massage as an independent method of treatment in the XIX–XX centuries.
Purpose. Studying the evolution of massage as one of the directions of manual medicine in Russia, interconnected with the mentality of our people and traditions that are rooted in the long past.
Materials and methods. The study was carried out through a theoretical method – the analysis of scientific literature on the history of massage, the history of medicine and, to some extent, the history of medical education. The authors in their theoretical study relied on historical and cultural approaches that allow us to consider the evolution of massage in Russian medicine.
Results. Theoretical analysis of scientific literature made it possible to visualize in a scientific text the evolution of massage as one of the directions of manual medicine in Russia.
Conclusions. The results of the theoretical research contribute to the history of medicine and medical education, are a source that allows us to rethink the contribution of Russian scientists to the development of massage as one of the directions of manual medicine in Russia.
PANDEMIC LESSONS: DIGITALIZING TRENDS ON MEDICAL EDUCATION IN THE COVID-19 ERA
Authors: Soloveva Irina A. , Yuryeva Elena A. , Kustova Tatyana V. , Belyaeva Anna V. , Tkachenko Oksana V. , Narkevich Artem N. 
Published: March 2023

The beginning of the COVID-19 pandemic and the response of the Institutes of health aimed at reducing the spread of the disease required an immediate change in the traditional approach to education, especially medical. Against the background of severe restrictions in the implementation of educational programs – normative, situational and managerial - universities have an acute question about the available opportunities for mastering the competencies of the educational standard in a remote format. Forced emergency changes in approaches, forms, and methods of training allowed overcoming the crisis in the field of medical education. The article highlights the main trends in medical education formed during the crisis associated with the new coronavirus infection. The experience of organizing training in a pandemic in an educational organization is presented.
The purpose of this work is to identify the main trends in the digitalization of medical education during the period of the threat of the spread of a new coronavirus infection.
Methods of comparative analysis, description and subject modeling of indicators recorded in the electronic educational environment of the University for the period of 2020 were applied, as well as information from the Portal of continuing medical and pharmaceutical education was used.
As a result, the main trends in the digitalization of medical education during the period of high alert on the example of COVID-19 were identified: the introduction of online education; personalization of medical education; transformation of working time and revision of process management practices at the university.
The identified trends have led to the preservation of the quality of training for practical health care and have become a new starting point in the strategy for the development of medical education.
DEVELOPMENT OF THE PRODUCTION OF OIL IN THE VOLGOGRAD REGION
Authors: Belikina Anna V. , Sukhareva Elena P. 
Published: March 2023

The Volgograd region is one of the largest regions producing sunflower oil seeds. In the approved State program of the Volgograd region “Development of agriculture and regulation of the markets of agricultural products, raw materials and food” until 2025, targets for the production and sale of sunflower seeds are fixed, and within the framework of the federal project “Export of agricultural products”, the share of the region in the volume of Russian exports of oil and fat products by 2024.
Purpose. Analyze the possibility of meeting the fixed targets in the state plans.
Materials and methods. In the process of writing the article, publicly available documents of state authorities, information on agricultural production of the State Statistics Committee of the Russian Federation and the Volgograd region were used. The work was carried out using the graphical-analytical method and simple extrapolation. Microsoft Office Excel 8 was used for the calculation work.
Results. In 2020, over 709 thousand hectares of sunflower crops were located in the region, and the yield of its seeds was 1.53 t/ha. The article considers the dynamics of the current state of sunflower seed production in the Volgograd region and the resource base on the basis of which the sub-sector operates. A forecast has been made, where it is determined that the indicator of the volume of sunflower seeds produced will be successfully achieved. Two scenarios are considered, according to which the development of sunflower seed production is possible and the results of their implementation are determined.
Conclusion. After analyzing the current state of production of sunflower oil seeds, we can conclude that the level of their production enshrined in the State Program will be successfully achieved. Scenarios for the implementation of the established goals are proposed.
STUDY OF BIOLOGICAL ACTIVITY COLLOIDAL SOLUTIONS OF IRON SYNTHESIZED ON THE BASIS OF AQUEOUS CUFF EXTRACT
Authors: Nikishina Maria B. , Ivanova Evgeniya V. , Tretyakova Anastasia V. , Mukhtorov Loik G. , Atroshchenko Yuri M. 
Published: March 2023

Background. Anthropogenic impact on agricultural areas leads to pollution of environmental objects, as well as to an increase in the resistance of pathogens to modern means of protection. One of the ways to solve these problems is the development of new drugs based on colloidal solutions of metals synthesized by the “green synthesis” method using aqueous extracts of medicinal plants from central Russia. The biological activity of metal nanoparticles obtained using plant extracts is currently being actively studied in relation to various biological objects. It is advisable to study the effect of iron colloids obtained with the help of an aqueous extract of the common mantle on the growth and development of wheat plants, as well as on phytopathogen fungi, the causative agents of the main diseases of agricultural plants.
Purpose. To study the biological activity of iron colloids synthesized on the basis of aqueous extracts of the common cuff. To establish the effect of iron nanoparticles on the growth and development of wheat plants, and to study the fungicidal effect of colloids on pathogenic fungi.
Materials and methods. Iron colloids were obtained from plant extracts of various dilutions and iron salt solution.
The content of sugars, tannins and dyes, photosynthetic pigments, vegetable protein, flavonoids and ascorbic acid was determined in the composition of the common cuff.
The influence of iron colloids on the growth and development of agricultural plants was studied by analyzing the seed germination energy and biometric parameters of wheat germs treated with initial extracts and synthesized colloids.
The fungicidal activity of common mantle extracts and iron colloids synthesized on their basis was studied in vitro on the following fungi – phytopathogens: F. moniliforme and F. oxysporum, V. inaequalis, R. solani, B. sorokiniana, P. ostreatus and A. alternata.
Results. Growth-stimulating activity is exhibited by aqueous extracts of the common cuff of an average degree of dilution and iron colloids synthesized on their basis. Only extracts of the common cuff have fungistatic properties in relation to the fungus B. sorokiniana.
Conclusion. Biological activity is shown by extracts of medium dilution and colloids synthesized on their basis. In further research on this topic, it is advisable to study the effect of various variations in salt and extract concentrations on the manifestation of fungicidal and growth-stimulating activity.
REVALENCE, EVALUATION CRITERIA AND DIAGNOSIS METHODS OF INTERNET ADDICTION
Authors: Moskalenko Olga L. , Tereshchenko Sergey Yu. , Kasparov Eduard V. 
Published: March 2023

Purpose: review of domestic and foreign studies in the field of Internet addiction.
Materials and methods. A review of the literature is presented and scientific studies on the prevalence of Internet addiction according to researchers from various countries are analyzed. The authors conducted a scientific search using the relevant keywords in the PubMed and Google Scholar search engines, in the Scopus, Web of Science, MedLine, The Cochrane Library, EMBASE, Global Health, CyberLeninka, RSCI (eLIBRARY.RU) and others databases.
Results. Internet addiction is widespread among children, adolescents and adolescents, and every year their number is increasing. In most of the studies conducted to identify internet addiction, the most accessible research methods were used – questionnaires, questionnaires. Individuals of early adolescence who are prone to Internet addiction have anxiety-depressive states, difficulties in doing work, and high excitability.
Conclusion. The results of studies of Internet addiction conducted in different countries of the world prove that this phenomenon has a worldwide prevalence and currently there are questions about the nosological affiliation, diagnostic criteria, clinical manifestations, and especially the prevention of Internet addiction.
PALEOECOLOGICAL CONDITIONS AND CARBON ACCUMULATION IN GENESIS OF INUNDATED MIRE OF MID-RUSSIAN UPLAND
Authors: Volkova Elena M. , Leonova Olga A. , Mironov Vasyliy V. 
Published: March 2023

The aim is to study of influence of the ecological conditions on the carbon accumulation at the different stages of genesis of the inundated mire.
Materials and methods. The macrofossil analysis and the degree of peat decomposition were studied by the microscopic method, the moisture content and the dry bulk density were studied by the gravimetric method. The ash content was determined by burning, the content of organic matter was calculated by the difference between the mass of dry peat and the ash content. The carbon content (%) in peat samples was calculated based on the dry bulk density, the organic matter content and the mass fraction of carbon for each volume of peat. The rate of vertical growth of peat and intensity of peat accumulation in periods of Holocene were calculated based on the results of radioactive dating.
The parameters of surrounding landscapes during the mire genesis were characterized by the results of the palynology analysis and the estimation of fire activity. The intensity of fires in paleolandscapes was studied by the content of coal particles in each (1 cm3) sample of wet peat.
Results. The genesis of the Podkosmovo mire is presented by 5 stages which were took place under the stable conditions of water-mineral nutrition. The carbon accumulation in the genesis proceeded at the rate of 45.7 g/m2/year on average. At the same time, the surrounding landscapes differed in the degree of afforestation and the intensity of anthropogenic press.
Conclusion. The moderate hydration and nutrition with mineralized waters ensure the stability of the mire development and the intensity of carbon accumulation, despite the change of surrounding landscapes under the influence of climate or human. However, the active transformation of the surrounding landscapes can have a negative impact on the functioning of the mire and reduce the intensity of carbon accumulation.