bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrical Burn and Associated Factors That Prolong In-patient Stay in a Level Three Burn Unit
Authors: Tróchez-Sanchez Juan Pablo , Garcia-Perdomo Herney Andres 
Published: March 2023

Abstract
Electrical trauma is associated with significant morbidity and mortality, which have been reduced by improved medical care, as measured by the length of stay (LOS) as a marker for this population’s quality of care. This paper will review the clinical and demographic characteristics, LOS in the hospital, and variables related to patients with electrical burns. A retrospective cohort study was conducted at a specialized burn unit in Southwest Colombia. Five hundred seventy-five electrical burn-related admissions from 2000 to 2016 were reviewed for the LOS and variables including patient-related (age, gender, marital status, education, and occupation), location of the accident (domestic vs labor-related), voltage, direct contact, arc, flash, flame, clinical presentation (burn surface area, depth, single or multiple organ injury, secondary infection, and abnormal labs), and treatment (surgical procedures and intensive care unit [ICU] admission). Univariate and bivariate analysis, with its 95% CI (confidence interval). We also performed a multiple logistic regression. LOS was correlated to males, age greater than 20 years, construction workers, high voltage injuries, severe burns by area and depth, infection, ICU admission, and multiple surgical procedures or extremity amputation. LOS due to electrical injury was observed to be significantly associated with the following variables, carpal tunnel release (OR [odds ratio]= 4.25, 95% CI [confidence interval] 1.70–5.20); amputation (OR = 2.81, 95% CI 1.60–5.10); infection (OR = 2.60, 95% CI 1.30–5.20); site of infection, mainly wound (OR = 1.30, 95% CI 1.10–1.44); associated injury (OR = 1.72, 95% CI 1.00–3.24); work or domestic accident (OR = 1.83, 95% CI 1.00–3.32); aged 20–40 years (OR = 1.41, 95% CI 1.00–2.10); CPK (OR = 1.40, 95% CI 1.00–2.00); and third-degree burns (OR = 1.55, 95% CI 1.00–2.80). Risk factors for LOS secondary to electrical injury should be appropriately addressed. Prevention at high-risk workplaces is imperative. Mitigating the injury with appropriate management of infection and timely surgical interventions play an essential role in the successful treatment of these patients.
Cryptic diversity in endoparasitic isopods (Bopyroidea: Entoniscidae) from mud crabs (Panopeidae) along the Atlantic coast of North America, with the description of a new genus and new species as revealed by molecular and larval characters: the long and the short of it
Authors: Williams Jason D , Boyko Christopher B , Tepolt Carolyn K , Blakeslee April M H 
Published: March 2023

AbstractAlthough intertidal crabs from the western Atlantic coast are relatively well studied for some parasite fauna, their parasitic isopods are poorly known. We document two endoparasitic entoniscids (Cancrion carolinusPearse & Walker, 1939 and Cryptocancrion brevibrachiumn. gen., n. sp.) from mud (Brachyura: Panopeidae) and stone (Brachyura: Menippidae) crabs based on molecular and morphological data, including descriptions of their epicaridium larvae. Although these species cannot be distinguished based on adult female characters, molecular data and larval and male morphologies show that two species are present, sometimes sympatrically. The only other species of CancrionGiard & Bonnier, 1887 from the Atlantic coast of North America is Cancrion needleriPearse & Walker, 1939, described from adult specimens parasitizing Dyspanopeus sayi (Smith, 1869) from Prince Edward Island, Canada. Larvae of this species were never described and we consider it a species inquirenda. Cryptocancrion brevibrachiumn. gen., n. sp. is documented to have epicaridium larvae with a “short-armed” morphology (sixth pereopod subequal in length to pereopods 1–5), and the adults parasitize at least three mud crabs: D. sayi, Panopeus herbstiiH. Milne Edwards, 1834, and Rhithropanopeus harrisii (Gould, 1841). By contrast, Cancrion carolinus (originally described from P. herbstii collected in Beaufort, NC, USA) has epicaridium larvae with a “long-armed” morphology (sixth pereopod approximately twice the length of pereopods 1–5) and is found in at least four host species: Eurypanopeus depressus (Smith, 1869), P. herbstii, and R. harrisii and the stone crab Menippe mercenaria (Say, 1818). We describe C. brevibrachiumn. gen., n. sp. and redescribe C. carolinus based on type material and fresh specimens. The cryptic nature of these entoniscids indicates that examination of epicaridium larvae and/or molecular data are needed to properly identify species.
Phylogenetic analysis of problematic Asian species ofArtemiaLeach, 1819 (Crustacea, Anostraca), with the descriptions of two new species
Authors: Asem Alireza , Yang Chaojie , Eimanifar Amin , Hontoria Francisco , Varó Inmaculada , Mahmoudi Farnaz , Fu Chun-Zheng , Shen Chun-Yang , Rastegar-Pouyani Nasrullah , Wang Pei-Zheng , Li Weidong , Yao Liping , Meng Xinyu , Dan Ya-Ting , Rogers D Christopher , Gajardo Gonzalo 
Published: March 2023

AbstractSpecies of Artemia are regionally endemic branchiopod crustaceans composed of sexual species and parthenogenetic lineages, and represent an excellent model for studying adaptation and speciation to extreme and heterogeneous hypersaline environments. We tested hypotheses of whether populations from the Tibetan Plateau belong to A. tibetianaAbatzopoulos, Zhang & Sorgeloos,1998 and whether a population from Kazakhstan is a new species, using other Asian species of Artemia as outgroups. We conducted a multitrait phylogenetic study based on the complete mitogenome, mitochondrial (COI, 12S, 16S) and nuclear (microsatellites, ITS1) markers, and a suit of uni- and multivariate morphological traits. Our results led to the discovery of two new species, one from the Tibetan Plateau (Haiyan Lake) in China (Artemia sorgeloosin. sp.) and a second from Kazakhstan (Artemia amatin. sp.). Our analysis demonstrate that A. tibetiana and A. amatin. sp. are monophyletic, whereas A. sorgeloosin. sp., and A. tibetiana are polyphyletic. Evolutionary relationships based on mitochondrial and nSSR markers suggest that A. tibetiana may have arisen from a past hybridization event of a maternal ancestor of A. tibetiana with A. sorgeloosin. sp. or its ancestor. We present the complete mitogenome of A. tibetiana, A. amatin. sp., and A. sorgeloosin. sp. We also provide a novel taxonomic identification key based on morphology, emphasizing the phenotype as a necessary component of the species concept.
Glucose- and disaccharide-containing baits impede secondary mortality in glucose-averse German cockroaches
Authors: McPherson Samantha , Wada-Katsumata Ayako , Silverman Jules , Schal Coby 
Published: March 2023

AbstractGlucose aversion in the German cockroach, Blattella germanica (L.), results in behavioral resistance to insecticidal baits. Glucose-averse (GA) cockroaches reject foods containing glucose, even in relatively low concentrations, which protects the cockroaches from ingesting lethal amounts of toxic baits. Horizontal transfer of baits and the resulting secondary mortality have been documented in German cockroaches, including in insecticide resistant strains. However, the effects of the GA trait on secondary mortality have not been investigated. We hypothesized that ingestion of insecticide baits that contain glucose or glucose-containing disaccharides would result in behaviorally relevant glucose levels in the feces, possibly deterring coprophagy by GA nymphs. We fed adult female cockroaches hydramethylnon baits rich in either glucose, fructose, sucrose, or maltose and compared secondary mortality of GA and wild-type (WT) nymphs via coprophagy. When adult females were fed baits containing glucose, sucrose, or maltose and their feces offered to nymphs, secondary mortality was significantly lower in GA nymphs than in WT nymphs. However, survival of GA and WT nymphs was similar on feces generated by adult females fed fructose bait. Analysis of feces indicated that disaccharides in baits were hydrolyzed into glucose, some of which was excreted in the feces of females that ingested the bait. Based on these results, we caution that baits containing glucose or glucose-containing oligosaccharides may impede cockroach interventions; while GA adults and large nymphs avoid ingesting such baits, first instars reject the glucose-containing feces of any WT cockroaches that consumed the bait.
Energy Geopolitics and Climate Law: Interdisciplinary Environmental Law Scholarship in a Geopolitical World
Authors: Boute Anatole 
Published: March 2023

Abstract
Energy geopolitics has received limited attention in the climate law scholarship, despite the importance of the energy sector for climate change and the impact of energy crises on the effectiveness of decarbonisation mechanisms. The shock caused by the current war in Ukraine shows that, in a geopolitical world, geopolitical analysis must be integrated into climate, and more generally environmental, law analysis.
Access to Data for Environmental Purposes: Setting the Scene and Evaluating Recent Changes in EU Data Law
Authors: Finck Michèle , Mueller Marie-Sophie 
Published: March 2023

Abstract
Few policy issues will be as defining to the EU’s future as its reaction to environmental decline, on the one hand, and digitalisation, on the other. Whereas the former will shape the (quality of) life and health of humans, animals and plants, the latter will define the future competitiveness of the internal market and relatedly, also societal justice and cohesion. Yet, to date, the interconnections between these issues are rarely made explicit, as evidenced by the European Commission’s current policy agendas on both matters. With this article, we hope to contribute to, ideally, a soon growing conversation about how to effectively bridge environmental protection and digitalisation. Specifically, we examine how EU law shapes the options of using data—the lifeblood of the digital economy—for environmental sustainability purposes, and ponder the impact of on-going legislative reform.
Risk, Reward, and Resilience Framework: Integrative Policy Making in a Complex World
Authors: Roberts Anthea 
Published: March 2023

ABSTRACT
In the last few years, many states around the world have faced a series of shocks to their economies—from the seizing up of global supply chains to the weaponization of trade and finance—that have catapulted concerns about how to strike a balance between risk, reward, and resilience into the center of public discussions and policy-making. This Article provides a new Risk, Reward, and Resilience Framework, which synthesizes and integrates insights from diverse disciplines and domains. I set out the drivers of each element, how they are connected, and resulting policy choices. To illustrate its practical application, I apply Risk, Reward, and Resilience Framework to two case studies involving economic interdependence: the COVID-19 supply chain shock and China’s economic coercion of Australia. In doing so, I provide policymakers with a new mental model for thinking about and working through complex policy challenges.
Phagocytosis via complement receptor 3 enables microbes to evade killing by neutrophils
Authors: Smirnov Asya , Daily Kylene P , Gray Mary C , Ragland Stephanie A , Werner Lacie M , Brittany Johnson Morgan , Eby Joshua C , Hewlett Erik L , Taylor Ronald P , Criss Alison K 
Published: March 2023

Abstract
CR3 (CD11b/CD18; αmβ2 integrin) is a conserved phagocytic receptor. The active conformation of CR3 binds the iC3b fragment of complement C3 as well as many host and microbial ligands, leading to actin-dependent phagocytosis. There are conflicting reports about how CR3 engagement affects the fate of phagocytosed substrates. Using imaging flow cytometry, we confirmed that binding and internalization of iC3b-opsonized polystyrene beads by primary human neutrophils was CR3-dependent. iC3b-opsonized beads did not stimulate neutrophil reactive oxygen species, and most beads were found in primary granule-negative phagosomes. Similarly, Neisseria gonorrhoeae that does not express phase-variable Opa proteins suppresses neutrophil reactive oxygen species and delays phagolysosome formation. Here, binding and internalization of Opa-deleted (Δopa) N. gonorrhoeae by adherent human neutrophils was inhibited using blocking antibodies against CR3 and by adding neutrophil inhibitory factor, which targets the CD11b I-domain. No detectable C3 was deposited on N. gonorrhoeae in the presence of neutrophils alone. Conversely, overexpressing CD11b in HL-60 promyelocytes enhanced Δopa N. gonorrhoeae phagocytosis, which required the CD11b I-domain. Phagocytosis of N. gonorrhoeae was also inhibited in mouse neutrophils that were CD11b-deficient or treated with anti-CD11b. Phorbol ester treatment upregulated surface CR3 on neutrophils in suspension, enabling CR3-dependent phagocytosis of Δopa N. gonorrhoeae. Neutrophils exposed to Δopa N. gonorrhoeae had limited phosphorylation of Erk1/2, p38, and JNK. Neutrophil phagocytosis of unopsonized Mycobacterium smegmatis, which also resides in immature phagosomes, was CR3-dependent and did not elicit reactive oxygen species. We suggest that CR3-mediated phagocytosis is a silent mode of entry into neutrophils, which is appropriated by diverse pathogens to subvert phagocytic killing.
Review of three neglected species of Ctenomys (Rodentia: Ctenomyidae) from Argentina
Authors: Sánchez R Tatiana , Tomasco Ivanna H , Díaz M Mónica , Barquez Rubén M 
Published: March 2023

Abstract
Ctenomys is one of the most speciose genera among South American rodents, whose systematics have not yet been fully elucidated. One of the difficulties for clarifying the identity of its species is the limited information available on some of them, erected more than a century ago and of which only the original descriptions are known, generally very poorly detailed. Our study provides new information about Ctenomys coludo Thomas 1920, based on the collection of topotype specimens that allowed us to perform more detailed morphological and molecular analyses, and to compare them with the available information referred to the holotype, with specimens deposited in museum collections, and other species of the genus that have been proposed as related to C. coludo. Our results allow us to place C. coludo as a member of the mendocinus group, proposing C. famosus Thomas, 1920 as a junior synonym for C. coludo. We also recognize Ctenomys johannis as a valid and full species, and provide unpublished information on it from the collection of specimens at the type locality. Thus, we add new areas for the distribution of these species and present their phylogenetic relationships.
Wei-Tong-Xin exerts anti-inflammatory effects through TLR4-mediated macrophages M1/M2 polarization and affects GLP-1 secretion
Authors: Zhang Xiaoying , Yang Xihan , Zhang Shuanglin , Wang Jinyu , Wang Mengshi , Ma Tiancheng , Wan Meiqi , Lv Xinyan , Yan Tingxu , Jia Ying 
Published: March 2023

Abstract

Objectives
The present study was undertaken to explore the effects and mechanisms of Wei-Tong-Xin (WTX) in inhibiting lipopolysaccharide (LPS)-induced inflammatory response of macrophages, in turn, to study the influences on GLP-1 secretion of GLUTag cells.

Methods
We first evaluated the activation of Raw 264.7 cells and measured the intracellular ROS, CD86 and CD206 levels by flow cytometry. The expressions of proteins were detected by western blot and immunofluorescence. GLP-1 levels were detected by ELISA kits. TLR4 siRNA was used to investigate the role of TLR4 in the regulation of macrophage polarization by WTX.

Key findings
The results showed that WTX inhibited LPS-induced polarization of macrophages toward the M1 phenotype, but promoted the M2 phenotype. Meanwhile, WTX inhibited the TLR4/MyD88 pathway. The polarization of M1 phenotype promoted GLP-1 secretion by GLUTag cells, which was inhibited by WTX. The results of siRNA showed that WTX exhibited anti-inflammatory effects through targeting TLR4.

Conclusions
Overall, WTX inhibited polarization of macrophages towards M1 phenotype but promoted the amounts of M2 phenotype, further the macrophages regulated by WTX alleviated GLP-1 content secreted by GLUTag cells. The aforementioned results were produced by WTX-mediated TLR4.
Radio-pathological diagnosis of a retroperitoneal cavernous hemangioma
Authors: Khazaal Ahmed S , Sultan Omar M , Rasheed Inas A A M 
Published: March 2023

AbstractRetroperitoneal cavernous hemangioma (RCH) is a rare benign vascular malformation. Only a few cases of RCH were reported. Here we present a case of RCH in a 66-year-old female complaining of long-standing progressive dull abdominal pain.
Methotrexate-associated lymphoproliferative disease detected as a colorectal mass lesions: a case report
Authors: Shirakabe Katsudai , Mizokami Ken 
Published: March 2023

AbstractMethotrexate-related lymphoproliferative disorder (MTX-LPD) is a rare but serious complication that occurs in patients treated with methotrexate (MTX); although MTX-LPD has been reported recently, the incidence in the colon is very low. A 79-year-old woman who had been receiving MTX for 15 years came to our hospital complaining of postprandial abdominal pain and nausea. Computed tomography scan showed the dilation of the small bowel and a tumor in the cecum. In addition, numerous nodular lesions were seen in the peritoneum. Ileal-transverse colon bypass surgery was performed for small bowel obstruction. Histopathological findings of both the cecum and the peritoneal nodules revealed the diagnosis of MTX-LPD. We report MTX-LPD occurring in the colon; it is important to consider MTX-LPD when intestinal symptoms occur during MTX therapy.
Metastatic small cell lung cancer presenting as acute appendicitis: a case report
Authors: Connell Louis , Ng Ziqin , Tai Wei Zhong , Allanson Ben 
Published: March 2023

AbstractWe present a case of extensive stage small cell lung cancer presenting as perforated appendicitis secondary to an appendiceal metastasis. This is a rare presentation with only six reported cases in the literature. Surgeons must be aware of unusual causes for perforated appendicitis as in our case the prognosis can be dire. A 60-year-old man presented with an acute abdomen and septic shock. Urgent laparotomy and a subtotal colectomy were performed. Further imaging suggested the malignancy was secondary to a primary lung cancer. Histopathology demonstrated a ruptured small cell neuroendocrine carcinoma in the appendix with thyroid transcription factor 1 positive immunohistochemistry. Unfortunately, the patient deteriorated due to respiratory compromise and was palliated day six postoperatively. Surgeons should consider a broad differential diagnosis for the cause of acute perforated appendicitis as this can rarely be due to a secondary metastatic deposit from a widespread malignant process.
A rare case of retropharyngeal liposarcoma: a rare location of a rare diagnosis
Authors: Arshad Wajiha , Maqbool Shahzaib , Kiany Javeria A , Raza Ali , Farooq Umer , Ali Qasim , Lee Ka Y 
Published: March 2023

Abstract
We report a case of retropharyngeal liposarcoma in a 53-year-old female, who had complaints of neck swelling accompanied with dysphagia, orthopnea and dysphonia. Clinical examination revealed huge multinodular swelling in front of neck with bilateral extension, more prominent on left side and moving with deglutition. The diagnosis of retropharyngeal liposarcoma was established following CT scan, MRI and incisional biopsy. Surgical excision of mass along with near total thyroidectomy was performed. Postoperative hospital stay was uneventful. She remained well in follow-up period of 1 year as well. In conclusion, retropharyngeal liposarcoma is a rare tumor. A review of the literature explores the reasons behind the late presentation as well as the difficulties in diagnosis and treatment of this rare tumor.
The role of SWEET4 proteins in the post-phloem sugar transport pathway of Setaria viridis sink tissues
Authors: Chen Lily , Ganguly Diep R , Shafik Sarah H , Danila Florence , Grof Christopher P L , Sharwood Robert E , Furbank Robert T 
Published: March 2023

Abstract
In the developing seeds of all higher plants, filial cells are symplastically isolated from the maternal tissue supplying photosynthate to the reproductive structure. Photoassimilates must be transported apoplastically, crossing several membrane barriers, a process facilitated by sugar transporters. Sugars Will Eventually be Exported Transporters (SWEETs) have been proposed to play a crucial role in apoplastic sugar transport during phloem unloading and the post-phloem pathway in sink tissues. Evidence for this is presented here for developing seeds of the C4 model grass Setaria viridis. Using immunolocalization, SvSWEET4 was detected in various maternal and filial tissues within the seed along the sugar transport pathway, in the vascular parenchyma of the pedicel, and in the xylem parenchyma of the stem. Expression of SvSWEET4a in Xenopus laevis oocytes indicated that it functions as a high-capacity glucose and sucrose transporter. Carbohydrate and transcriptional profiling of Setaria seed heads showed that there were some developmental shifts in hexose and sucrose content and consistent expression of SvSWEET4 homologues. Collectively, these results provide evidence for the involvement of SWEETs in the apoplastic transport pathway of sink tissues and allow a pathway for post-phloem sugar transport into the seed to be proposed.
The glutathione-dependent alarm triggers signalling responses involved in plant acclimation to cadmium
Authors: Iven Verena , Vanbuel Isabeau , Hendrix Sophie , Cuypers Ann 
Published: March 2023

Abstract
Cadmium (Cd) uptake from polluted soils inhibits plant growth and disturbs physiological processes, at least partly due to disturbances in the cellular redox environment. Although the sulfur-containing antioxidant glutathione is important in maintaining redox homeostasis, its role as an antioxidant can be overruled by its involvement in Cd chelation as a phytochelatin precursor. Following Cd exposure, plants rapidly invest in phytochelatin production, thereby disturbing the redox environment by transiently depleting glutathione concentrations. Consequently, a network of signalling responses is initiated, in which the phytohormone ethylene is an important player involved in the recovery of glutathione levels. Furthermore, these responses are intricately connected to organellar stress signalling and autophagy, and contribute to cell fate determination. In general, this may pave the way for acclimation (e.g. restoration of glutathione levels and organellar homeostasis) and plant tolerance in the case of mild stress conditions. This review addresses connections between these players and discusses the possible involvement of the gasotransmitter hydrogen sulfide in plant acclimation to Cd exposure.