bluuebunny/crossref_metadata_2025_split huggingface dataset:
Staged Open Cranial Surgery for Primary Intra-axial Neoplasms: A Systematic Review
Authors: Bui Nick , Brown Nolan , Gendreau Julian , Patel Neal , Shahrestani Shane , Patel Saarang , Beyer Ryan , Koester Stefan , Singh Rohin , Abraham Mickey , Mammis Antonios 
Published: February 2023

Abstract
Background
Staged surgery for skull base lesions has been utilized to facilitate maximal safe resection and optimize outcomes while minimizing morbidity and complications. Conversely, staged surgery for primary intraparenchymal neoplasms is less commonly performed and has not been reported as extensively within the literature. As such, we performed a systematic review to examine the unique surgical indications for staging, timing between stages, specific surgical approaches employed, and postoperative complications of staged surgery for primary intra-axial neoplasms.
Methods
A literature search was conducted in August 2021 using PubMed, Web of Science, and Cochrane databases using the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) recommendations. Titles and abstracts were evaluated independently by two authors, after which articles were selected for final analysis based on application of strict inclusion criteria during full text screen. Each included article was then qualitatively assessed and relevant variables – including operative approaches, timing, and outcomes – were extracted for synthesis.
Results
Of 115 results, 7 articles were included for final analysis and consisted of 17 pediatric and 4 adult patients. Staged approaches were more commonly utilized in the pediatric patient population for resection of astrocytoma and glioma. Pediatric patients had a timing of surgeries ranging from 5–10 days between operations, compared to 18 days-4 months in adult patients. Complications in pediatric patients were most commonly hemiparesis, hydrocephalus, CN VI and VII palsies, truncal ataxia, and cerebellar mutism, while complications in adult patients included language and abstract thinking deficits, respiratory failure, and motor weakness.
Conclusion
This study reports the first comprehensive review of staged surgical procedures for primary, intra-axial cranial neoplasms. There exists a large degree of heterogeneity in complications resulting from staged surgeries for intra-axial neoplasms, which are similar to complications associated with single-stage surgery for intraparenchymal lesions as well as multi-stage surgeries for skull base lesions.
EDUVI: An Educational-Based Visual Question Answering and Image Captioning System for Enhancing the Knowledge of Primary Level Students
Authors: Gupta Manisha , Asthana Priya , Singh Preetvanti 
Published: February 2023

Abstract
Within the last several years, the revolution in online education has fundamentally transformed the idea of traditional education. The trend of online education is exploding in popularity in the modern educational system. However, it is challenging for primary-level students to adapt to learning in this novel environment. When enrolled in online courses, students at this level encounter a variety of difficulties. Hence, to provide better learning methods, this research study focuses on developing an education based EDUVI system for primary level students which helps the students in visual learning. The proposed system will help the students for self-learning without any assistance using a simplified and interactive platform. The developed system integrates visual question answering and image captioning system where students can import the image and extract answer based on the query asked by them or can generate the description or caption.
Agomir miRNA-150-5p alleviates pristane-induced lupus by suppressing myeloid dendritic cells activation and inflammation via TREM-1 axis
Authors: Yue Chenran , Gao Sheng , Ye Jianzhong , Zhang Ting , Xing Zhouhang , Xie Yuanyuan , Qian Hengrong , Zhou Xueyin , Li Shuting , Yu Anni , Wang Liangxing , Hua Chunyan 
Published: February 2023

Abstract
Triggering receptors expressed on myeloid cell-1 (TREM-1) has been shown to participate in inflammatory autoimmune diseases. Nevertheless, the detailed underlying mechanisms and therapeutic benefits remain elusive, especially in myeloid dendritic cells (mDCs) and systemic lupus erythematosus (SLE). In this study, we screen TREM-1 as one of the hub genes closely correlated with the progression of SLE and identify soluble TREM-1 (sTREM-1) in serum as a valuable diagnostic biomarker for SLE. Moreover, activation of TREM-1 by its agonist promotes activation and chemotaxis of mDCs and increases production of inflammatory cytokines and chemokines, showing higher expression of IL-6, TNF-α and MCP-1. Disorders of epigenetic processes including non-coding RNA give rise to SLE, resulting in complicated syndromes. Herein, we show that lupus mice display a unique miRNA signature in spleen, among which miR-150 is the most significantly expressed miRNA that targeting TREM-1 compared with wild type group. Transfection of miRNA-150-5p mimics directly suppresses the expression of TREM-1 by binding to its 3' UTR. Our in vivo experiments first indicates that administration of miR-150-5p agomir effectively ameliorates lupus symptoms. Intriguingly, miR-150 inhibits the over activation of mDCs through TREM-1 signal pathway in lymphatic organs and renal tissues. Overall, TREM-1 represents a potentially novel therapeutic target and we identify miR-150-5p as one of the mechanisms to alleviate lupus disease, which is attributable for inhibiting mDCs activation through TREM-1 signaling pathway.
Synthesis, antileishmanial, antimalarial evaluation and molecular docking study of some hydrazine-coupled pyrazole derivatives
Authors: Berhe Halefom Gebreselasse , Birhan Yihenew Simegniew , Beshay Botros Youssef , Habib Huda Jawad , Hymete Ariaya , Bekhit Adnan Ahmed 
Published: February 2023

Abstract
Pyrazole-bearing compounds are known for their diverse pharmacological effects including potent antileishmanial and antimalarial activities. Herein, some hydrazine-coupled pyrazole derivatives were successfully synthesized and their structures were verified by employing elemental microanalysis, FTIR, and 1H NMR techniques. The in vitro antileishmanial and in vivo antimalarial activities of the synthesized pyrazole derivatives (9–15) were assessed using the clinical isolate of the Leishmania aethiopica strain and mice infected with Plasmodium berghei ANKA strain, respectively. The result revealed that compound 13 displayed superior antipromastigote activity (IC50 = 0.018) that was 174- and 2.6-fold more potent than the standard drugs miltefosine (IC50 = 3.130) and amphotericin B deoxycholate (IC50 = 0.047). The molecular docking study conducted on Lm-PTR1 justified the better antileishmanial activity of compound 13. Furthermore, the target compounds 14 and 15 elicited better inhibition effects against Plasmodium berghei with 70.2% and 90.4% suppression, respectively. In conclusion, the hydrazine-coupled pyrazole derivatives may be considered potential pharmacophores for the synthesis of safe and effective antileishmanial and antimalarial agents.
Da Vinci robot versus thoracoscopic surgery for lymph node dissection in radical lung cancer: a propensity score matching study
Authors: Hong Ziqiang , Sheng Yannan , Bai Xiangdou , Cui Baiqiang , Wang Kai , Wu Xusheng , Cheng Tao , Jin Dacheng , Gou Yunjiu 
Published: February 2023

Abstract
Objective: To compare the efficacy of robot-assisted thoracoscopic surgery (RATS) with video-assisted thoracoscopic surgery (VATS) in lymph node dissection for radical lung cancer.
Methods: Retrospective analysis of 262 minimally invasive surgically treated lung cancer patients who underwent radical lung cancer surgery via RATS (n=122) or VATS (n=140) completed by the same operator in the Department of Thoracic Surgery, Gansu Provincial People’s Hospital from August 2019 to August 2022. Propensity score matching (PSM) analysis was performed to compare the procedure-related information of the two groups of patients.
Results: All patients in both groups successfully completed radical lung cancer surgery with no perioperative deaths. There are 100 patients in each group after PSM. The RATS group had an advantage in terms of intraoperative bleeding, postoperative drainage time, postoperative hospital stay, number of lymph nodes cleared (6, 10, 11 stations), number of lymph nodes cleared, and escalation of lymph node pathological stage (cN0 to pN1). In terms of total postoperative chest drainage and hospital costs, the VATS group was more advantageous. There was no statistically significant difference between the two groups in terms of operative time, postoperative complications and intraoperative intermediate chest opening (P>0.05).
Conclusion: RATS has technical and short-term efficacy advantages, especially in lymph node dissection, but has the disadvantage of expensive.
Involvement of the excretory/secretory and surface-associated antigens of Dirofilaria immitis adult worms in the angiogenic response in an in-vitro endothelial cell model
Authors: Machado Cristian David Cardona , Alarcón-Torrecillas Claudia , Pericacho Miguel , Rodríguez-Escolar Iván , Carretón Elena , Montoya-Alonso José Alberto , García Rodrigo Morchón 
Published: February 2023

Abstract
Background: Angiogenesis is a process by which new vessels are formed from pre-existing ones when the physiological conditions of the vascular endothelium are altered. Heartworm disease (Dirofilaria immitis) causes changes in the vascular endothelium of the pulmonary arteries due to obstruction, friction and hypoxia. The aim of this study was to analyze whether the excretory/secretory antigen of adult worms interacts and modulates the angiogenic mechanism, viable cell number and cell migration, as well as the formation of pseudo-capillaries.
Methods: Cultures of human vascular endothelial cells (HUVECs) stimulated with excretory/secretory antigens (DiES), surface-associated antigens (Cut) from D. immitis adult worms, VEGF, as well as DiES+VEGF and Cut+VEGF were used. The production of VEGF-A and other proangiogenic [soluble VEGFR-2 (sVEGFR-2), membrane Endoglin (mEndoglin)] and antiangiogenic [VEGFR-1/soluble Flt (sFlt), soluble Endoglin (sEndoglin)] molecules was assessed using commercial ELISA kits. Cell viability was analyzed by live cell count and cytotoxicity assays by a commercial kit. In addition, viable cell number by MTT-based assay, cell migration by wound-healing assay carrying out scratched wounds, and the capacity of pseudo-capillary formation to analyze cell connections and cell groups in Matrigel cell cultures, were evaluated. In all cases, non‑stimulated cultures were used as controls.
Results: DiES+VEGF and Cut+VEGF significantly increased the production of VEGF and VEGFR2, and only Cut+VEGF significantly increased the production of VEGFR1/sFlt compared to other groups and non-stimulated cultures. Moreover, only DiES+VEGF produced a significant increase in viable cell number and cell migration, as well as in the organization and number of cell connections.
Conclusions: Excretory/secretory and surface-associated antigens of adult D. immitis activated the angiogenic mechanism by mainly stimulating the synthesis of proangiogenic factors, and only excretory/secretory antigens increased viable cell number, activated cell migration and the formation of pseudo-capillaries. These processes could lead to vascular endothelial remodeling of the infected host and favor the long-term survival of the parasite.
The composition of Gut microflora of stingless honey bee (Apidae: Tetragonula spp.) from Dharangaon taluka of Jalgaon district
Authors: Nikam Varun B. , Chopda Manojkumar. Z. , Salunkhe Hemant. P. , Mahajan Namrata G. , Talele Bharati D. , Sonawane Deveshri S. , Maheshwari Nayan R. 
Published: February 2023

Abstract
Stingless bees are the main pollinators in tropical regions because they entirely rely on flowers for nectars and pollen to feed their larvae. They help in pollinating many forests and are valued pollinators in biodiversity. Tetragonula /Trigona is known as the largest genera of stingless bees comprising 32 species worldwide and (meliponini) is by far the largest group of eusocial bees on earth with more than 500 described species and 43 species occurring in the Asian region. In a sterile environment, we surface-sterilized the bees in 75% alcohol for 20 s and then washed them with sterile water. Finally, the entire gut was removed from the abdomen of the bee. The sample was homogenized with one ml of Insect saline solution. 16S RNA analysis was performed by outsourcing service from the Rajiv Gandhi Center for Biotechnology, Thiruvananthapuram. The results showed that the gut of stingless bees contained the bacteria of the Priestia genus
Choosing to Lie: Strategic and Pathological Deception
Authors: Corlett Philip , Rossi-Goldthorpe Rosa 
Published: February 2023

Abstract
Humans lie to one another frequently with local and global consequences. Here we examine and operationalize the choice to lie using a mathematical model which we fit to participant behavior. This model combines perceptions of exploitability and fairness attitudes to explore the interaction between two types of utility: instrumental utility and moral utility. These utilities differed in concert with individual differences in participants’ behavioral traits, and those differences underwrote lies with distinct motivations. Strategic deception – wherein the lie benefits the individual - was common amongst people with elevated subclinical psychopathic traits in the absence of paranoia. It was driven by differences in instrumental utility. Whereas pathological deception – which has no apparent economic or strategic benefit – was common among highly paranoid participants (who also had elevated scores on dark triad personality traits) and was governed by the moral utility of the lie. They lied for little apparent benefit because they failed to modulate their fairness attitudes in line with the decision context. These data challenge accounts of deception that suggest deceiving oneself is a prerequisite to successful lying. They also oppose accounts of psychopathology that connect psychopathic traits to pathological lying, and theories that implicate strategic social decision-making dysfunction in paranoia. More broadly, these data highlight the importance of individual differences in efforts to mitigate deception, which have thus far, largely focused on incentive structures and decision architectures.
Outbreaks of Severe Acute BVDV in Quebec, Ontario and New York State, Occurring Between 1993 and 1995, Linked to the Same Strain of Type 2 BVDV
Authors: Ridpath Julia F. , Neill John D. 
Published: February 2023

In this study we compared the virulence and genetic sequence of four BVDV strains to each other, and to strains isolated in North America from 1989 to 1996.
Preliminary Validation of a 'Calf-Side' Test for Measurement of Serum IgG in Dairy Calves
Authors: Stewart S. , Godden S. , Schrupp M. 
Published: February 2023

The importance of adequate passive transfer (APT, IgG ≥ 10 mg/mL at 24-48 h) of immunoglobulin (lg) for newborn calves is widely known in the dairy industry. Failure of passive transfer (FPT) is associated with increased risk of morbidity, mortality, reduced growth rate, and decreased production at first lactation. The gold standard test is measurement of serum IgG concentrations in an accredited veterinary laboratory using either RID or turbidimetric immunoassay (TIA) methods. Laboratory measures are accurate but expensive ($6 to $12 per sample), plus take time to ship samples and wait for results. A common on-farm test is to measure serum total protein (TP) concentrations using a hand held refractometer to indirectly predict serum IgG concentrations. This approach is inexpensive and rapid but is less accurate than direct IgG measurement in a laboratory. Calloway and others (2002) reported that serum TP concentrations of 5.0 g/dL had a sensitivity and specificity of 0.89 and 0.84, respectively, in detecting calves with FPT. Ideally, the industry would have available an accurate, rapid, convenient, and inexpensive calf-side test to measure serum IgG concentrations. The Midland BioProducts QTII™ Bovine Serum IgG test is a portable hand-held chemistry analyzer that works on the principle of a TIA method (Midland BioProducts Inc., Boone, IA). The chemistry analyzer weighs about 2 lb (0.91 kg), is about the size of a 1 L bottle and costs $1,950.00 as a one time purchase. Test kits are available in sets of 5 at approx. $7 .50 per test for the bovine species IgG. Test kits are also available for other bovine analytes including serum albumin, total protein, calcium, magnesium and phosphorus. It takes approx. six minutes to run one test. The objective of this study was to describe the test characteristics of the MBC QTII™ Bovine Serum IgG test.
Treat the Treatable and Manage the Rest
Authors: Welcome Frank , Zurakowski Mike , Welch Gerald , Schukken Ynte 
Published: February 2023

Farm income for many dairies can be improved by increasing the value of milk by taking advantage of somatic cell count (SCC) based milk quality premiums and reducing treatment costs. Recent research has shown that the likelihood of an animal to have a repeat case of clinical mastitis after a first clinical case is about 30%. Pathogen-based treatment protocols should provide a science-based process for selecting the most appropriate treatment and duration of treatment resulting in high bacteriological cure rates and fewer repeated clinical cases. Many infections caused by gram-positive organisms, particularly streptococcal species, respond well to appropriate treatment; cure rates can approach 80% or higher. Most gram-negative pathogens respond poorly to intramammary treatment. Mastitis caused by other pathogens, such as yeast, are exacerbated by intramammary treatment and others, like Prototheca species and mycoplasma, have no known treatment. Generally the normal distribution of mastitis pathogens is about one-third gram-positive organisms, one-third gram-negative pathogens, and one-third no growth culture result, suggesting that pathogen-based treatment protocols have the potential for substantial savings.
Duration of treatment for specific mastitis pathogens should have a major influence on cure rates, particularly for a range of streptococcal species and select Staphylococcus aureus infections.
There are also opportunities to manage chronic subclinical infections which are often the major contributors to the bulk tank SCC. Milk from high cell count cows can be managed through treatment, segregation of high SCC milk at the quarter level, quarter dry-off, and culling.
Bovine Coronavirus Infections in Transported Commingled Beef Calves and Sole-source Ranch Cattle
Authors: Fulton Robert W. , Step D. L. , Wahrmund Jackie , Burge L. J. , Eberle R. , D'Offay J. M. , Confer Anthony W. , Cook Billy , Burken Dick , Richards Chris , Payton Mark 
Published: February 2023

Bovine respiratory diseases (BRD) occurring in the feedlot represent the major disease entity during the feeding period. Several bacteria, viruses, and Mycoplasma spp are reported as causative agents. Feedlot BRD may occur at various times, although the early disease appearing after arrival and processing often receives the most attention. In addition to bovine herpesvirus-1 (BHV-1), bovine viral diarrhea viruses (BVDV), bovine respiratory syncytial virus (BRSV), bovine parainfluenza-3 virus (PI-3V), bovine coronavirus (BC) infections have been found in BRD cases. Potentially the case can be made for the use of vaccines to control BCV disease. There are no USDA APHIS CVB licensed vaccines for respiratory disease in the US.
The purpose of this study was to: 1) determine the presence of BCV in cattle entering the feedlot from auction markets commingled and shipped to the feedlot and the dynamics of infection over time, and 2) determine the BCV antibody levels in ranch raised (direct from the ranch and not commingled with other cattle) cattle entering the feedlot.
Impact of Ceftiofur Use on the Recovery of Salmonella and Resistant E. coli in Dairy and Beef Cattle Populations
Authors: Kleinhenz K. E. , Mollenkopf D. F. , Heider L. C. , Funk J. A. , Wittum T. E. 
Published: February 2023

The use of the third generation cephalosporin, ceftiofur, is common in animal agriculture for the treatment of a wide range of production-limiting disease conditions. The therapeutic use of ceftiofur in livestock can select for the blaCMY-2 genetic element in the intestinal flora. The presence of resistant organisms is a potential public health threat to the consumer. This project investigated the impact of ceftiofur removal from cattle populations and the meat products they produced.
The working hypothesis is that the use of ceftiofur in food animal populations selects for the emergence and rapid dissemination of third generation cephalosporin resistant microorganisms, leading to the potential risk of food-borne zoonotic pathogen transfer via the food chain.
From pasture to package
Authors: Hatcher Charles W. 
Published: February 2023

The dairy industry in the United States has undergone significant structural change in the last 50 years. In Williamson County, Tennessee, which is my home county, there were over 500 dairies in the county during the 1950s. Now there are 4, and the Hatcher Family Dairy is one of those 4 remaining dairies.
This is the story of how our farm family reached for the retail dollar to save the dairy.
Keys to successful robotic milking systems
Authors: Salfer Jim , Endres Marcia 
Published: February 2023

The use of robotic milking systems is growing rapidly in North America. Most installs have been on smaller farms that desire an improved lifestyle and to minimize the need to hire labor. The majority of robotic milkers are single box systems where one robot arm services one milking box, but some newer designs are multi-box where one robotic arm will service two to five milking boxes.
The two types of cow traffic in robotic milking systems are guided flow and guided flow. In free flow cow have free access to feed, the lying area and the milking robot. Guided flow systems have pre-selection gates that help guide cows that need milking to the robot.
There are several keys to making robotic systems successful. One major factor is that successful producers really like working with cows. Robotic milking systems require excellent management for success. Other major factors that drive success include feeding management and well-designed facilities that facilitates excellent cow flow and efficient labor management. It is important that producers develop realistic cash flows. Taking a whole system approach in design and management will maximize the RMS performance.
Bacterial isolates and factors associated with infection and outcome in calves with septic arthritis
Authors: Constant C. , Nichols S. , Fecteau G. , Babkine M. , Larde H. , Francoz D. 
Published: February 2023

Lameness is an important problem in cattle and is often associated with an important economic loss in beef and milk production. Because of the pain, it is becoming an important welfare issue and represents 1 of the major culling causes in dairy herds. The joint is the second most important cause of lameness after the digit.
There is in an important lack in the literature regarding prognosis and factors associated with septic arthritis in calves. The objective of the study is to determine clinical characteristics, clinicopathologic data, and bacterial culture results associated with septic arthritis in calves less than 180 days old and to establish long-term prognosis of the condition.