bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Combinational Therapy Using Topical Minoxidil and Microneedling for The Treatment of Androgenetic Alopecia: A Systematic Review and Meta-Analysis
Authors: Abdi Parsa , Awad Christian , Anthony Michelle R. , Farkouh Christopher , Kenny Bret , Maibach Howard 
Published: March 2023

Abstract
Androgenetic alopecia is a widespread condition that is the most common type of hair loss affecting approximately 58% and 40% of men and women by the age of 50, respectively. Patients have been known to experience severe distress due to androgenetic alopecia, including anxiety, low self-esteem, and depression. The objective of this study was to conduct a systematic review and meta-analysis to determine the efficacy of combination therapy using topical minoxidil and microneedling compared to topical minoxidil alone.
This systematic review of randomized controlled trials was carried out using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist and was registered in the International Prospective Register of Systematic Reviews (PROSPERO) database (CRD42023391164). The literature search was performed using Scopus, Cochrane, Embase, and the National Institutes of Health's United States National Library of Medicine from inception through January 20, 2022. Randomized controlled trials examining the efficacy of combinational therapy and monotherapy using microneedling and minoxidil on patients with clinically diagnosed androgenetic alopecia were included after screening titles, abstracts, and full texts. Two independent reviewers selected studies, extracted data, and appraised the risk of bias using the Cochrane risk of bias assessment tool. Ten randomized controlled trials, including ­­­466 patients,­ were selected for this review and eight studies were ultimately included in the meta-analysis. All eight studies displayed a statistically significant increase in total hair count (standard mean difference [SMD], 1.76; 95% CI, 1.26-2.26; P<0.00001); however, the evidence did not support a statistically significant increase in hair diameter (SMD, 0.82; 95% CI, -0.01–1.65; P=0.05). No scarring nor serious adverse events were reported in any of the studies.
The findings of this meta-analysis strongly support the utilization of a multimodal therapeutic approach of minoxidil and microneedling for hair growth in patients with androgenetic alopecia. However, variations in factors such as rating scale measurements, microneedling methods, and areas of treatment may have resulted in confounding. Further randomized controlled, large-sample trials employing rigorous methodologies are needed to gain a more comprehensive understanding regarding treatment efficacy, namely the impact of combinational therapy on hair diameter.
Integrative single-cell transcriptome analysis reveals new insights into post-COVID-19 pulmonary fibrosis and potential therapeutic targets
Authors: Kim Yumin , Lim Hyobin , Kim Dae-Kyum , Park Ji-Hwan , Oh Chang-Myung 
Published: March 2023

Abstract
The global COVID-19 pandemic caused by the SARS-CoV-2 virus has resulted in a significant number of patients experiencing persistent symptoms, including post-COVID pulmonary fibrosis (PCPF). This study aimed to identify novel therapeutic targets for PCPF using single-cell RNA-Sequencing data from lung tissues of COVID-19 patients, idiopathic pulmonary fibrosis (IPF) patients, and a rat TGF-β1-induced fibrosis model treated with antifibrotic drugs. Patients with COVID-19 had lower alveolar macrophage counts than healthy controls, whereas patients with COVID-19 and IPF presented with elevated monocyte-derived macrophage counts. A differential gene expression analysis showed that macrophages play a crucial role in IPF and COVID-19 development and progression, and fibrosis- and inflammation-associated genes were upregulated in both conditions. Pathway analysis revealed upregulation of inflammation and proteolysis and downregulation of ribosome biogenesis and respiratory gas exchange. Cholesterol efflux and glycolysis were augmented in both macrophage types. The study suggests that antifibrotic drugs may reverse critical lung fibrosis mediators in COVID-19. The results help clarify the molecular mechanisms underlying pulmonary fibrosis in patients with severe COVID-19 and IPF and highlight the potential efficacy of antifibrotic drugs in COVID-19 therapy. Thus, the study's results may have significant implications for the development of new treatment strategies for PCPF.
Degradation of methylene blue in aqueous phase by homogenous and heterogeneous catalytic Ozonation
Authors: Mohammadi Ali Akbar , Kamarehie Bahram , Jafari Ali , Hosseingholizade Nasrin , Ghaderpoori Mansour , Ahmadpour Mohammad , Baloch Muhammad Yousuf Jat 
Published: March 2023

Abstract
This study presented the removal of methylene blue in the batch system based on heterogeneous and homogenous catalytic ozonation. The effect of different variables such as initial (methylene blue) MB concentration, solution pH (3–11), contact time (5–40 min), and catalyst dosage on homogeneous and heterogeneous ozonation systems was examined. The findings of this research showed that MB removal was better in alkaline conditions. The amount of removed MB in acidic conditions was slightly better than in neutral conditions (approximately 10–15%). In addition, the removal efficiency of MB was decreased by increasing of initial concentration. Besides, as the amount of catalyst increased, the removal efficiency had been increased by 40%. The significant difference between the use of two processes of single ozonation processes (SOP) and catalytic ozonation processes (COPs) was due to the use of catalysts. Based on the findings, MB degradation in COPs was several times faster than SOP (approximately 4.7 times). It was due to the numerous advantages of these processes in the removal of environmental pollutants, they can be widely used in the removal of various pollutants if their cost can be reduced.
Application of GIS and feedforward back-propagated ANN models for predicting the ecological and health risk of potentially toxic elements in soils in Northwestern Nigeria
Authors: Omang Benjamin Odey , Omeka Michael Ekuru , Asinya Enah Asinya , Oko Peter Ereh , Aluma Victor Chukwuemeka 
Published: March 2023

Abstract
Potentially toxic elements occur naturally in most geologic materials. However, recent anthropogenic disturbances such as ore mining have contributed significantly to their enrichment in soils. Their occurrence in soil may portend a myriad of related risks to the environment and biota. Most traditional soil quality evaluation methods involve comparing the background values of the elements to the established guideline values, which is often time-consuming and fraught with computational mistakes. As a result, in order to conduct a comprehensive and unbiased evaluation of soil quality and its effects on the ecosystem and human health, this research combined geochemical, numerical, and GIS data for a composite health risk zonation of the full study region. Furthermore, the multilayer perceptron artificial neural network was used to forecast the most important toxic components influencing soil quality (MLP-NN). Geochemical, statistical, and quantitative soil pollution evaluation (pollution index and ecological risk index) showed that apart from mining, the spread and association of trace elements and oxides occur as a consequence of surface environmental conditions (e.g., leaching, weathering, and organo-metallic complexation). The hazard quotients (HQs) and hazard index (HI) of all PTEs were greater than one. This indicates that residents (particularly children) are more susceptible to risks from toxic components ingestion than skin exposure and inhalation. Ingestion of As and Cr resulted in higher cancer risks and lifetime cancer risk levels (> 1.0E 04), with risk levels increasing towards the northeastern, western, and southeastern directions of the study area. The low modeling errors observed from the sum of square errors, relative errors, and coefficient of determination confirmed the efficiency of the MLP-NN in pollution load prediction. Based on the sensitivity analysis, Hg, Sr, Zn, Ba, As, and Zr showed the greatest influence on soil quality. Focus on remediation should therefore be placed on the removal of these elements from the soil
What can Peano say about ‘2+3=5’?
Authors: Serény György 
Published: March 2023

Abstract
In a recent paper, Mary Leng expressed her conviction that the equality `2+3=5' should be justified by its derivability from the Peano axioms. It turns out, however, that proving `2+3=5' from the Peano axioms and counting apples in the appropriate collections are essentially the same game played with different pieces. Indeed, generally, proofs of simple arithmetical equalities from the Peano axioms can be shown to be really empirical demonstrations disguised as axiomatic proofs. Moreover, the other frequently used attempt to justify such equalities in a non-empirical way also fails.
Groundwater potentiality mapping using machine learning algorithms BouSbaa area, Marrakech, Morocco
Authors: Hanane Toudamrini , Ahmed Algouti , Abdellah Algouti 
Published: March 2023

AbstractGroundwater recharge is crucial for managing freshwater resources. Machine learning algorithms are used to discuss the important aspects of groundwater exploration. For maximum accuracy, Extreme Gradient Boosting and Random Forest have been tested for modeling groundwater potential maps. A database of water point inventories has been prepared, randomly divided into 75% for training and 25% for model validation. A database of flows is used to confirm the feasibility of the model. Groundwater potential maps are generated using various relevant factors (elevation, slope, precipitation, etc.). After validation of the model using ROC-AUC and confirmation of feasibility with flow diagrams, these methods have shown high accuracy and relevant results for groundwater potential models.
Positive differences of overpartitions with separated parts
Authors: Bachraoui Mohamed El 
Published: March 2023

Abstract
In this note we investigate partition differences of overpartitions where overlined and non-overlined part are separated. As a consequence of our main results, we deduce a variety of positivity results for overpartition differences.
Date: March 2, 2023 .
2000 Mathematics Subject Classification. 11P81; 05A17; 11D09.
The implication of Epstein-Barr virus latent membrane protein 1 on the composition and prognostic significance of the tumor microenvironment of classical Hodgkin lymphoma
Authors: Zawati Imen , Adouni Olfa , Manai Maroua , Reduzzi Carolina , Nagara Majdi , Tacam Moisis , Hamdeni Tasnime , Gamoudi Amor , Manai Mohamed 
Published: March 2023

Abstract
Purpose Epstein-Barr virus (EBV) is crucial for the pathogenesis of classical Hodgkin lymphoma (CHL). In this study, we evaluated whether the EBV could predict the clinical behavior of CHL and impact the tumor microenvironment(TME) signatures.
Methods We conducted immunohistochemical analyses for the expression of EBV-latent membrane protein 1 (LMP1) and markers reflecting immune cells, including CD3, CD4, CD8, FOXP3, CD20, and CD68 in 102 CHL samples and calculated the ratios between cell subsets for each specimen.
Results EBV-LMP1 positivity was detected in 35 (34%) cases and remained an independent poor prognostic factor for overall survival (OS) (p = 0.022). In addition, the CD8/FOXP3+ ratio was significantly increased in LMP1+ than in LMP1- tumors (p = 0.027). Using Cox modeling, a high CD8/CD68+ ratio was associated with shorter OS in LMP1+ tumors compared to a low CD8/CD68+ ratio (p = 0.039); however, neither CD8+ nor CD68+ separately was correlated to the CHL survival. Moreover, the Kaplan-Meier method showed an association of a high CD8/CD68+ ratio with inferior OS in LMP1+ tumors (p  = 0.013). In multivariate analysis, a high CD8/CD68+ ratio and B-symptoms tended to be independent prognostic factors for OS (HR = 9.80 (0.56-171.5), p = 0.11; HR = 10.28 (1.00-106.0), p= 0.05; respectively).
Conclusion EBV-LMP1+ is an independent unfavorable prognostic factor for CHL, modulating the TME towards a cytotoxic profile. Besides, CD8/CD68+ ratio could be a potential factor acccruately predicting the clinical outcomes of LMP1+ CHL patients, providing more prgnostic information than each cell seperately.
Predation avoidance behavior and web investment are not constrained by individual nutritional status in a shelter-building spider species
Authors: Ceribelli Paloma , Moura Rafael Rios , Kloss Thiago Gechel 
Published: March 2023

Abstract

Optimal foraging and individual specialization theories suggest that different properties of the interaction between prey and predators determine foraging strategies. However, none of them consider how the nutritional status of the predators and the risk of being attacked by other predators can affect the option for these strategies. Some spider species can build webs as dynamic traps to capture prey and perform behaviors that optimize capture efficiency while adopting strategies to minimize their exposure to predators, such as building a shelter. In this sense, the risk of predation during foraging and the nutritional status of spiders may be important factors mediating predator-prey interactions, because capturing prey involves leaving the shelter, which may contribute to an increased risk of exposure to predators lurking close to the web. In this study, we evaluated the hypotheses that individuals of the shelter-building spider Metazygia laticeps (Araneidae) with poor nutritional status (i) are more exposed to predators during foraging and (ii) invest more in web structures to capture prey. Nutritional status was unrelated to exposure to predation. However, M. laticeps individuals left the shelter faster at night than during the day, regardless of their nutritional status, probably to reduce exposure to visually oriented predators. In addition, individuals with poor nutritional status did not show higher investment in webs when compared to well-nourished spiders. Nutritional status, therefore, was not a determining factor modulating investment in web foraging structures by M. laticeps.
Identification of potential molecular targets for the imaging and treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization
Authors: Vit Ondrej , Talacko Pavel , Musil Zdenek , Hartmann Igor , Pacak Karel , Petrak Jiri 
Published: March 2023

Abstract
Background: Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors. New drug targets and proteins that would assist sensitive PPGL imagining could improve therapy and quality of life of patients with PPGL, namely those with recurrent or metastatic disease. Using a combined proteomic strategy, we looked for such clinically relevant targets among integral membrane proteins (IMPs) upregulated on the surface of tumor cells and non-membrane druggable enzymes in PPGL.
Methods: We conducted a detailed proteomic analysis of 22 well-characterized human PPGL samples and normal chromaffin tissue from adrenal medulla. A standard quantitative proteomic analysis of tumor lysate, which provides information largely on non-membrane proteins, was accompanied by specific membrane proteome-aimed methods, namely glycopeptide enrichment using lectin-affinity, glycopeptide capture by hydrazide chemistry, and enrichment of membrane-embedded hydrophobic transmembrane segments.
Results: The study identified 67 cell surface integral membrane proteins strongly upregulated in PPGL compared to control chromaffin tissue. We prioritized the proteins based on their already documented direct role in cancer cell growth or progression. Increased expression of the seven most promising drug targets (CD146, CD171, ANO-1, CD39, ATP8A1, ACE and SLC7A1) were confirmed using specific antibodies. Our experimental strategy also provided expression data for soluble proteins. Among the druggable non-membrane enzymes upregulated in PPGL, we identified three potential drug targets (SHMT2, ARG2 and autotaxin) and verified their upregulated expression.
Conclusions: Application of a combined proteomic strategy recently presented as “Pitchfork” enabled quantitative analysis of both, membrane and non-membrane proteome, and resulted in identification of 10 potential drug targets in human PPGL. Seven membrane proteins localized on the cell surface and three non-membrane druggable enzymes proteins were identified and verified as significantly upregulated in PPGL. All the proteins have been previously shown to be upregulated in several human cancers, and play direct role in cancer progression. Marked upregulation of these proteins along with their localization and established direct roles in tumor progression make these molecules promising candidates as drug targets or proteins for sensitive PPGL imaging.
Self-Initiation Enhances Perceptual Processing of Auditory Stimuli in an Online Experiment
Authors: Kiepe Fabian , Kraus Nils , Hesselmann Guido 
Published: March 2023

Abstract
Understanding how the brain incorporates sensory and motor information will enable better theory building on human perception and behavior. In this study, we aimed to estimate the influence of predictive mechanisms on the magnitude and variability of sensory attenuation in an online sample. After the presentation of a visual cue stimulus, participants (N = 224) compared the loudness of two consecutive tones in a two-alternative forced choice task. The first tone was either self-initiated or not (active and passive condition, respectively). We further manipulated identity prediction (i.e., the congruence of pre-learned cue-sound combinations; congruent versus incongruent), and the duration of the sound offset (to account for effects of attentional differences between the passive and active condition, 50ms versus 0ms). Contrary to our preregistered hypothesis, we observed enhanced perceptual processing, instead of attenuation, for self-initiated auditory sensory input. Further, our results reveal an effect of fixed sound delays on the processing of motor and non-motor-based predictive information, and may point to according shifts in attention, leading to a perceptual bias. These results challenge previous explanatory models of sensory attenuation and suggest that the role of motor and non-motor-based predictions may need to be reevaluated.
Culturally responsive elementary science teaching: A meta-analysis of current science teaching studies and implications
Authors: Pinneo Lundon , Benton Amy L 
Published: March 2023

Abstract

This study utilizes the meta-analysis method described by Timulak (2009) to create a more cohesive understanding of culturally responsive (CR) science teaching. This phenomenon is explored by defining the characteristics of CR teaching from the extant literature and comparing those characteristics to current CR science teaching studies with a focus on the elementary level. Six themes emerge from the selected studies, representing both the extant literature and the current CR science teaching studies. As this study demonstrates, additional themes of CR science teaching are not represented in the extant literature. Additionally, no one study addresses all six themes. This paper will make the argument to include these six themes in future studies, which will result in more cohesive findings and lead to evidence-based strategies for implementing CR teaching in elementary preservice teacher settings.
A study on reservoir space type of Ordovician ultra-deep marine carbonate reservoir in T-Sh Oilfield, Tarim basin
Authors: Kang Yuan , Xiang Min , Liu Shuang , An Ran , Yang Fanfan , Zhang Fengwei , Zhang Xuhui 
Published: March 2023

Abstract
The T-Sh reservoir is a typical ultra-deep marine carbonate reservoir with numerous large strike-slip faults. While the large strike-slip faults are preferred as the drilling target because it has previously been understood that they are the main reservoir space. In the Sh-1 and Sh-5 fault zones, the application outcome of this understanding is effective, but invalid in the Sh-7 fault zones. Therefore, the permeability of Ordovician Yijianfang Formation faults in the Sh-5 and Sh-7 fault zone have been analyzed by using geomechanics theory and the finite element method. Meanwhile, the reservoir space types in various T-Sh reservoir regions in conjunction with mud loss during drilling have been evaluated. It is found that in Sh-1 and Sh-5 fault zones, the main reservoir space is the fault, and the critical Ts (slip tendency coefficient) of the Sh-5 fault zone is 0.2. However, it is of high-permeability micro-fracture groups-type in the Sh-7 fault zone reservoir. This discovery explains why the Sh-7 fault zone cannot benefit from the development experience learned from the Sh-1 and Sh-5 fault zones. The breakthrough of these results could serve as a reference for the T-Sh oil field's further exploration and development.
Inhibition of Lipid peroxidation by ALR protects the kidney from ischemia-reperfusion injury
Authors: Huang Lili , Zhang Ling , Tan Fangyan , Ma Yixin , Zeng Xujia , Cao Dan , Deng Lili , Liu Qi , Sun Hang , Zhang Zheng , Liao Xiaohui 
Published: March 2023

Abstract
Background
Ferroptosis, characterized by lipid accumulation in intracellular compartments, is related to acute kidney injury (AKI), but the mechanism remains obscure. In our previous study, we reported important roles for augmenter of liver regeneration (ALR) in antioxidant mechanisms. However, the roles of ALR in ferroptosis, especially the morphological changes in mitochondria induced by this type of regulated cell death, remain unclear and warrant further investigation.
Methods
We subjected Kidney-specific deletion of the ALR gene (ALR-K-KO), as well as HK-2 cells, to ischemia-reperfusion (I/R) induced AKI models. We assessed the kidney function and ferroptosis of proximal tubular epithelial cells. We also examined the level of lipid peroxidation by MS/MS. ALR and Long chain acyl-CoA synthetase 4 (ACSL4) were colocalized and interacting regions were detected by protein docking-analyses.
Results
Here, we hypothesize that ALR regulates oxylipin accumulation in proximal tubular cells and attenuates ferroptosis induced by ischemia-reperfusion (I/R) injury in AKI. Kidney-specific deletion of the ALR gene (ALR-K-KO) aggravated ferroptosis, accompanied by increased ROS production and mitochondrial damage, whereas overexpression of the ALR gene attenuated lipid accumulation. Moreover, acsl4 loss reduced mostly polyunsaturated fatty acids. In addition, ALR and ACSL4 colocalize in the mitochondria of HK-2 cells and protein docking analysis found the interacting regions.
Conclusion
We showed for the first time that ALR binds to ACSL4 and regulates ferroptosis in proximal tubular cells by attenuating oxylipin accumulation.