bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive review on the photocatalysis of Congo red dye for wastewater treatment
Authors: Siddique Nida , Din Muhammad Imran , Khalid Rida , Hussain Zaib 
Published: August 2023

Abstract
Congo red (CR) dye, due to its structural stability and non-degradable nature, is hazardous for humans and the aquatic environment. Among various methods used for degradation of CR, photocatalysis have been widely reported as a cost effective and environment-friendly method. Besides, extensive studies have been carried out regarding the use of nanomaterial-based photocatalyst for degradation of CR. This review describes the basics of photocatalysis along with the factors affecting the process, mechanism, and kinetics in detail. Additionally, literature related to synthetic and bio-based stabilizing mediums in photocatalytic assemblies have been arranged in a systematic manner. Advanced hybrid materials, i.e., metal–organic frameworks (MOFs) have also been described briefly. Future directions are discussed to address existing shortcomings in this field and also to expand research in this area.
Altered Thalamocortical Signaling in a Mouse Model of Parkinson's Disease
Authors: Swanson Olivia K. , Yevoo Priscilla E. , Richard Dave , Maffei Arianna 
Published: August 2023

Activation of the primary motor cortex (M1) is important for the execution of skilled movements and motor learning, and its dysfunction contributes to the pathophysiology of Parkinson's disease (PD). A well-accepted idea in PD research, albeit not tested experimentally, is that the loss of midbrain dopamine leads to decreased activation of M1 by the motor thalamus. Here, we report that midbrain dopamine loss altered motor thalamus input in a laminar- and cell type-specific fashion and induced laminar-specific changes in intracortical synaptic transmission. Frequency-dependent changes in synaptic dynamics were also observed. Our results demonstrate that loss of midbrain dopaminergic neurons alters thalamocortical activation of M1 in both male and female mice, and provide novel insights into circuit mechanisms for motor cortex dysfunction in a mouse model of PD.SIGNIFICANCE STATEMENTLoss of midbrain dopamine neurons increases inhibition from the basal ganglia to the motor thalamus, suggesting that it may ultimately lead to reduced activation of primary motor cortex (M1). In contrast with this line of thinking, analysis of M1 activity in patients and animal models of Parkinson's disease report hyperactivation of this region. Our results are the first report that midbrain dopamine loss alters the input–output function of M1 through laminar and cell type specific effects. These findings support and expand on the idea that loss of midbrain dopamine reduces motor cortex activation and provide experimental evidence that reconciles reduced thalamocortical input with reports of altered activation of motor cortex in patients with Parkinson's disease.
Racionalidad, ética y crítica en el humanismo de la praxis de Franz Hinkelammert
Authors: Acosta Yamandú 
Published: August 2023

Se reflexiona sobre las relaciones entre racionalidad, ética y crítica en el humanismo de la praxis de Franz Hinkelammert en relación a su comprensión sobre estas relaciones en la crítica de Marx al capitalismo. A este propósito se presentan dos versiones de un mismo texto de Hinkelammert sobre Marx en el que esta cuestión es abordada. La comparación de las dos versiones permite calibrar la complejidad del problema, su elaboración y definitiva resolución en Hinkelammert.
En el marco de la que se entiende como una actualización y ampliación por parte de Hinkelammert de la crítica de Marx se considera el papel de la ética tanto en la construcción y legitimación del capitalismo como en su crítica. La crítica al neoliberalismo como profundización y totalización del capitalismo en la actualidad, la crítica al socialismo y la crítica a la sociedad moderna occidental desarrolladas por Hinkelammert, hacen parte de la actualización y ampliación de Marx, que se aborda.Por la mediación del “sujeto de la praxis” y su “humanismo de la praxis” se considera la perspectiva crítico-constructiva alternativa al capitalismo, al socialismo y a la sociedad moderna-occidental, que sobre el criterio de la “racionalidad reproductiva” del hombre y la naturaleza hace a la perspectiva de la ética universal del “universalismo del hombre concreto” que Hinkelammert propone.
L’allegoria svelata: elementi misteriosofici ed esegesi Porfiriana nell’Antro delle ninfe
Authors: Furlan Anna Lucia 
Published: August 2023

AbstractCover and uncover: is the attempt to unveil and reveal the hidden meaning of the mysteries a paradox (Porph. Antr. 4. 16–17)? The present contribution aims at exploring Porphyry's interest in the commentary Περὶ τοῦ ἐν Ὀδυσσείᾳ τῶν νυμφῶν ἄντρου (Cave of the Nymphs) for the allegorical interpretation of images, symbols and terms variously linked with the Mysteries and encapsulating truths on the nature of the divine, the soul and ultimately the cosmos. More specifically, attention will be drawn mostly (but not only) on features connected with Orphism, here defined and understood as a form of ‘mysteriosophy’. The scope of this analysis falls within the study of Porphyry's use of allegorical exegesis but also within the broader discussion of different forms of philosophical religion in Late Antiquity, underscoring the complex but fruitful relationship between theology, philosophy and the Mysteries.
CO-SEGREGATED POLYNOMIAL OF GRAPHS
Authors: Tapeing Aziz B. , Laja Ladznar S. 
Published: August 2023

A graph $G$ is co-segregated if $\text{deg}_G(x)=\text{deg}_G(y),$ then $xy \in E(G)$. The co-segregated polynomial of a graph $G$ of order $n$ is given by $CoS(G,x)=\sum_{k=1}^{n}C(k)x^k$, where $C(k)$ is the number of co-segregated subgraphs of $G$ of order $k$. We characterize a co-segregated subgraph of a graph and also of a graph under some binary operations. Using these characterizations, we obtain co-segregated polynomials of such graphs.
Metagenomic Study on the Influence of Enterocytozoon hepatopenaei (EHP) Infection on the Gut Microbiota in Penaeus vannamei
Authors: Babu S. Ganesh , Uma A. , Kumar K. Anbu , Shanmugam S.A. , Pandian A. Kathirvel 
Published: August 2023

Background: Enterocytozoon hepatopenaei (EHP), infects P.vannamei and causes hepatopancreatic microsporidiasis associated with severe growth retardation in shrimp culture. The influence of EHP in the shrimp gut microbiota is poorly studied and this would be an interesting area to investigate. In this study, metagenomic approach was followed to compare the overall species richness of the gut microbiota in healthy and EHP-infected P. vannamei. Methods: Bacterial genomic DNA from gut samples of EHP-infected and healthy shrimps were profiled for the 16S rRNA gene, targeting the V3-V4 conserved region. Operational Taxonomic Units (OTUs), were identified and clustered together with a cutoff of 97% identity using UCLUST. The OTUs were then used for the computation of alpha diversity and beta diversity for each sample. Result: Gut samples from EHP-infected shrimp showed lower bacterial abundance throughout the family, class, order, and genus levels. This research also highlights that EHP not only affects the hepatopancreas of the shrimp, but it also has the ability to affect the shrimp gut, predisposing them to other opportunistic infections.
Nutritional Performance of Five Citrus Rootstocks under Different Fe Levels
Authors: Pestana Maribela , García-Caparros Pedro , Saavedra Teresa , Gama Florinda , Abadía Javier , Varennes Amarilis de , Correia Pedro José 
Published: August 2023

Iron is an essential micronutrient for Citrus, playing an important role in photosynthesis and yield. The aim of this paper is to evaluate the tolerance to Fe deficiency of five Citrus rootstocks: sour orange (S), Carrizo citrange (C), Citrus macrophylla (M), Troyer citrange (T) and Volkamer lemon (V). Plants were grown for 5 weeks in nutrient solution that contained the following Fe concentrations (in µM): 0, 5, 10, 15 and 20. At the end of the experiment, biomass (dry weight), leaf area, total leaf chlorophyll (CHL) and the activity of root chelate activity (FCR) were registered. Additionally, the mineral composition of roots (R) and shoots (S) was evaluated. Principal Component Analysis was used to study the relationships between all parameters and, subsequently, the relations between rootstocks. In the first component, N-S, P-S, Ca-S, Cu-S, Zn-S, Mn-S and Zn-R and Mn-R concentrations were related to leaf CHL and FCR. Increases in leaf CHL, Mg-R, DW (shoots and roots) were inversely related to Cu-R, which was shown in the second component. The values obtained were consistent for V10, C15, and C20, but in contrast for S0 and S5. Overall, the Fe/Cu ratio was significantly related to CHL, which may be used to assist rootstock performance.
Determination of oxidative stress in 3-dimensional reconstructed human tissues grown at the air-liquid interface using Promega’s GSH/GSSG-Glo™ Assay kit, using single lysate preparations
Authors: Patel Vivek S. , Wahab Adam , Behrsing Holger P. 
Published: August 2023

Abstract
Glutathione is an endogenous antioxidant known for its protective role in various oxidative stress-mediated diseases. It is abundantly present in all human cell types and its levels, reduced (GSH) and oxidized (GSSG) are frequently used to determine the oxidative state of biological systems (Marrocco et al., 2017). It is the ratio of these biomarkers that provide more comprehensive oxidative stress information about the tissues, as opposed to other kits measuring only one. While GSH/GSSG assay kits are available from several manufacturers, Promega’s GSH/GSSG-Glo™ Assay kit seems to be highly sensitive and can be used with a low number of cells. However, the Promega assay is designed to measure total glutathione (tGSH) and GSSG in cell lysate samples from different wells or separate 3-dimensional (3D) tissues (reconstructed human tissues grown at the air-liquid interface (ALI)) or precision-cut tissue slices, with different lysis reagents to be used for assessments of each molecule. Using this assay for 3D tissues is not cost-effective as researchers would need to double the number of tissues for the experiment just for this single oxidative stress ratio endpoint. In this protocol, we describe a simple lysis method for 3D tissues that enables the use of a single tissue for measuring both GSH and GSSG using the GSH/GSSG-Glo™ Assay. The 3D tissues can be lysed using sulfosalicylic acid and then the lysate supernatants can be used immediately or stored for use at a later time. Before running the Promega assay, the samples must be neutralized using HEPES buffer and diluted as necessary to get the signal within a linear range. The samples can then be used for measuring the tGSH and GSSG using Promega’s assay procedure. The entire procedure can be completed within 5 hours.
Evaluation of the stress reactive countermeasure effectiveness in workplace: a randomized, controlled, cross-over study
Authors: Ráčková Lucie , Diamond Madison , Butin Inès , Boyer Stanislas , Boyer Laure , Chabani Emma 
Published: August 2023

Abstract
This protocol serves to examine the effectiveness of a workplace-based reactive stress countermeasure (e.g., an action or device intended to prevent or mitigate undesirable effects of acute stress). It’s designed specifically for the context where decrease of acute stress reaction is desired while preserving vigilance and avoiding sleepiness. The study adopts a cross-over, controlled design. The order of interventions is randomized and blinded to the data collector and data analyst. This approach ensures increased validity and reliability of research outcomes while minimizing the number of participants required. The protocol employs affordable state-of-the-art subjective indicators measured using questionnaires, as well as objective physiological indicators of arousal (heart rate, respiration rate, electrodermal activity, frontal EEG). Baseline stress levels are measured, followed by induced acute stress using the Maastricht Acute Stress Task, and subsequent sessions of either countermeasure or control. Data analysis includes descriptive, time series, and ANCOVA or general linear models approaches. The protocol is estimated to take approximately 2 to 2.5 hours for completion.
scNanoHi-C: a single-cell long-read concatemer sequencing method to reveal high-order chromatinstructures within individual cells
Authors: Li Wen , Lu Jiansen , Tang Fuchou 
Published: August 2023

Abstract
The high-order three-dimensional (3D) organization of regulatory genomic elements provides a topological basis for gene regulation, but it remains unclear how multiple regulatory elements across the mammalian genome interact within an individual cell. To address this, herein, we developed scNanoHi-C, which applies Nanopore sequencing to explore genome-wide proximal high-order chromatin contacts within individual cells. Evaluation of the method suggested that scNanoHi-C reliably and effectively profiled 3D chromatin structure and distinguished structure subtypes among single cells. This method could also be used to detect genomic variations,including CNVs and SVs, as well as to scaffold the de novo assembly of single-cell genomes. Importantly, our results suggested that extensive high-order chromatin structures exist in active chromatin regions across the genome, and multiway interactions between enhancers and their target promoters were identified within single cells. Altogether, scNanoHi-C offers new opportunities to investigate high-order 3D genome structures at the single-cell level.
The SAFE Procedure: A Practical Stopping Heuristic for Active Learning-Based Screening in Systematic Reviews and Meta-Analyses
Authors: Boetje Josien , de Schoot Rens van 
Published: August 2023

Abstract
Active learning has become an increasingly popular method for screening large amounts of data in systematic reviews and meta-analyses. The active learning process continually improves its predictions on the remaining unlabeled records, with the goal of identifying all relevant records as early as possible. However, determining the optimal point at which to stop the active learning process is a challenge. The cost of additional labeling of records by the reviewer must be balanced against the cost of errors made by the current model. This paper introduces the SAFE procedure, a practical and conservative set of stopping heuristics that offers a clear guideline for determining when to end the active learning process in screening software like ASReview. Our main conclusion of this paper is that relying on a single stopping rule is not sufficient and employing an eclectic mix of stopping heuristics helps to minimize the risk of missing relevant papers in the screening processThe SAFE procedure combines different heuristics to avoid stopping too early and potentially missing relevant records. The SAFE procedure takes into account the model's accuracy and uncertainty, as well as the cost of continuing to label records. This procedure for using active learning in systematic literature review screening provides a practical and efficient approach that can save significant time and resources while ensuring a conservative approach to determining when to end the active learning process. The proposed stopping heuristic balances the costs of continued screening with the risk of missing relevant records, providing a practical solution for reviewers to make informed decisions on when to stop screening. The proposed method can assist researchers in identifying relevant records early, which can ultimately lead to improved evidence synthesis and decision-making in many fields.
Clinical Evaluation of MyoCare in Europe (CEME): study protocol for a prospective, multicenter, randomized, double-blinded, and controlled clinical trial.
Authors: Alvarez-Peregrina Cristina , Sanchez-Tena Miguel Angel , Martinez-Perez Clara , Villa-Collar Cesar , Group Clinical Evaluation of MyoCare in Europe the CEME Study , Ohlendorf Arne 
Published: August 2023

Abstract
Background
Myopia prevalence has been increasing in the last decades and its pathological consequences, including myopic maculopathy and high myopia-associated optic neuropathy, are now one of the most common causes of visual impairment. It is estimated that by 2050 more than 50% of Europeans and Americans, which is alarming due to the high morbidity of myopes over − 6.00D. Once myopia has appeared, there are different options with scientific evidence to try to slow the axial length growth. Ophthalmic lenses are the less invasive treatment to control myopia, and there is evidence about the efficacy of different designs, mainly in the Asiatic population. However, new designs have been launched and it is not known if efficacy is the same between Asiatic and European subjects. Thus, we have setup a randomized, controlled, double-blind, and multicenter trial to investigate the efficacy of a new design of ophthalmic lenses for myopia control in European children.
Methods
A 2-year prospective, multicenter, randomized controlled, and double-blind clinical trial is used to investigate the efficacy of a new design of ophthalmic lenses to slow the progression of myopia. 300 children aged from 6 to 13 years old will be recruited and randomly assigned to a study or control group. The study group will be composed of 150 children wearing MyoCare while the control group will be composed of 150 children wearing Clearview. The inclusion criteria will be myopia with a spherical equivalent between − 0.75D and − 5.00D, astigmatism < 1.50D, and anisometropia < 1.00D, as well as having a historical evolution of at least − 0.50 The primary outcome is to compare the mean annual progression of the spherical equivalent between both groups. The secondary outcomes are axial length, choroidal thickness, phorias, and accommodative status of both groups.
Discussion
This study will be the first randomized and controlled clinical trial in European children with spectacle lenses based on simultaneous competing defocus. The results will shed light on the clinical evidence of spectacle lenses relying on this new design for the management of myopia with results of efficacy in the non-Asiatic population.
Trial registration: EU Clinical Trials Register, EudraCT: 2022 − 001696. Registered on 27 April 2022, and ClinicalTrials.gov, ID NCT05919654. Registered on 26 June 2023
12-month outcomes of treatment-naïve retinal vein occlusion initially treated with either dexamethasone implants or vascular endothelial growth factor inhibitors: Data from the Fight Retinal Blindness! registry
Authors: GARAY-ARAMBURU GONZAGA , Hunt Adrian , Arruabarrena Carolina , Mehta Hemal , Invernizzi Alessandro , Gabrielle Pierre-Henry , Guillaumie Tremeur , Wolff Benjamin , Gillies Mark , Zarranz-Ventura Javier 
Published: August 2023

Abstract
Aim: To compare baseline characteristics, initial response and 12-month efficacy and safety outcomes in eyes with branch and central retinal vein occlusion (BRVO and CRVO) initially treated with either dexamethasone implants (DEX) or vascular endothelial growth factor (VEGF) inhibitors where both are available as first-line therapy.
Methods: Multi-centre study from European Fight Retinal Blindness! centres using the retinal vein occlusion module in routine clinical care.
Results: Of 725 eligible eyes, only 10% received DEX initially with very frequent adjunctive VEGF inhibitors (BRVO-DEX 49%, CRVO-DEX 60%). The primary outcome of mean adjusted change in VA at 12 months with DEX and VEGF inhibitors initiated groups were not statistically significantly different (BRVO: DEX +6.7, VEGF +10.6 letters; CRVO: DEX +2.8, VEGF +6.8 letters). DEX initiated eyes had fewer injections and visits than VEGF inhibitors initiated eyes but intraocular pressure required treatment more often in BRVO with DEX than VEGF inhibitors. We found the BRVO-DEX eyes had greater initial mean changes in VA and central subfield thickness (CST) and achieved inactivity sooner than BRVO-VEGF eyes. The mean CST after the first three months was above 350μm in all but the BRVO-VEGF group, suggesting undertreatment in routine care.
Conclusion:In routine care DEX is uncommonly used when available as initial treatment of BRVO and CRVO and is often supplemented with VEGF inhibitors within the first year. The 12-month outcomes were similar, but DEX initiated eyes did have fewer injections and visits but more episodes of raised IOP compared with those starting VEGF inhibitors.
Origin of the Laurentian Great Lakes fish fauna through upward adaptive radiation cascade prior to the Last Glacial Maximum
Authors: Backenstose Nathan , Macguigan Daniel , Osborne Christopher , Bernal Moisés , Thomas Elizabeth , Normandeau Eric , Yule Daniel , Stott Wendylee , Ackiss Amanda , Albert Victor , Bernatchez Louis , Krabbenhoft Trevor 
Published: August 2023

Abstract

The evolutionary histories of adaptive radiations can be marked by dramatic demographic fluctuations. However, the demographic histories of ecologically-linked co-diversifying lineages remains understudied. The Laurentian Great Lakes provide a unique system of two lineages that are dispersed across depth gradients with a predator-prey relationship. We show that the North American Coregonus species complex radiated rapidly prior to the Last Glacial Maximum (80–90 ka), a globally warm period, followed by rapid expansion in population size. Similar patterns of demographic expansion were observed in the predator species, Salvelinus namaycush, following a brief time lag, which we hypothesize to be driven by predator prey dynamics. Diversification of prey into deepwater created ecological opportunities for the predators, facilitating their demographic expansion through an upward adaptive radiation cascade. This study provides a new timeline and environmental context for the origin of the Laurentian Great Lakes fish fauna, and firmly establishes this system as drivers of ecological diversification and rapid speciation through cyclical glaciation.
Effects of sex steroids on the regulation of pituitary gonadotropins in the pituitary and hypothalamus
Authors: Yacca Susdiaman S , Kanasaki Haruhiko , Tumurbaatar Tuvshintugs , Cairang Zhuoma , Oride Aki , Okada Hiroe , Kyo Satoru 
Published: August 2023

Abstract
Sex steroids play a pivotal role in the control of the hypothalamic-pituitary-gonadal axis. In this study, we examined how the sex steroids estradiol (E2), progesterone (P4), and dihydrotestosterone (DHT) influence the synthesis of three pituitary gonadotropin subunits in a pituitary gonadotroph cell model (LβT2 cells) in vitro and ovary-intact rats in vivo. The effects of sex steroids on Kiss1 gene expression in the hypothalamus were also examined in ovary-intact rats. E2 increased common glycoprotein alpha (Cga) and luteinizing hormone beta (Lhb) subunit promoter activity as well as their mRNA expression. Although gonadotropin subunit promoter activity was not modulated by P4, Cga and Lhb mRNA expression was increased by P4. DHT inhibited Cga and Lhb mRNA expression with a concomitant decrease in their promoter activity. During the 2-week administration of exogenous E2 to ovary-intact rats, the estrous cycle, which was determined by vaginal smears, was disrupted. P4 or DHT administration completely eliminated the estrous cycle in rats. Protein expression of all three gonadotropin subunits within the pituitary gland was inhibited by E2 or P4 treatment in vivo; however, DHT reduced Cga expression but did not modulate Lhb or follicle-stimulating hormone beta subunit expression. E2 administration significantly repressed Kiss1 mRNA expression in a posterior hypothalamic region that included the arcuate nucleus. P4 and DHT did not modulate Kiss1 mRNA expression in this region. In contrast, P4 administration significantly inhibited Kiss1 mRNA expression in a posterior region of the hypothalamus that included the anteroventral periventricular nucleus. Our findings suggest that sex steroids have different effects in the hypothalamus and pituitary gland.