bluuebunny/crossref_metadata_2025_split huggingface dataset:
InSAR-based method for deformation monitoring of landslide source area in Baihetan reservoir, China
Authors: Liu Meishan , Yang Zhiquan , Xi Wenfei , Guo Junqi , Yang Hong 
Published: August 2023

As a cascading disaster, the surge caused by the reservoir bank landslide seriously affects the stability of the reservoir bank and the dam body. In addition, large-scale hydropower projects are usually built in mountain and canyon areas with active geological structure movement, which provides rich material sources for the occurrence of landslides, so it is of great significance to monitor the deformation in the landslide source area of the reservoir. As science and technology have been leaping forward, a wide variety of high and novel technologies have been proposed, which can be adopted to monitor landslide deformation. It is noteworthy that InSAR is capable of monitoring target monitoring areas all time under all weathers without the need to install any equipment. In this study, the time series deformation of the main landslide source area of the Baihetan reservoir after water storage was determined based on the time series InSAR method. The average annual deformation rate of the landslide source area of the Baihetan reservoir from April 2021 to January 2023 was determined by combining the Sentinel-1 SAR data of 55 ascending tracks and 46 descending tracks. Moreover, the vegetation cover variations from April 2021 to January 2023 in the study area were determined by combining the remote sensing data of Landsat8-9. A total of four typical source areas were selected based on the field investigation to analyze the deformation monitoring results and the vegetation cover variations. As indicated by the results: 1) After water storage, the slope deformation in all source areas was larger in the short term, and the deformation rate of the lower part turned out to be more significant, and the deformation rate exceeded 334.583 mm/year. 2) On the steep slope, the effect of different types of vegetation on restraining deformation was different. The optimal effect was reported in shrubs, followed by grasslands, and the worst effect was reported in woodlands. The results of this study can provide scientific support for the prevention and control of regional geological disasters.
Anti-diabetic combination therapy with pioglitazone or glimepiride added to metformin on the AGE-RAGE axis: a randomized prospective study
Authors: Ragazzi Eugenio , Burlina Silvia , Cosma Chiara , Chilelli Nino Cristiano , Lapolla Annunziata , Sartore Giovanni 
Published: August 2023

IntroductionThe ratio between advanced glycation end products (AGEs) and soluble form of receptor (s-RAGE) has been proposed as a risk marker for renal and cardiovascular diseases. The aim of this study was to evaluate in the diabetes condition the influence of two different oral anti-diabetic treatments on the AGE/s-RAGE ratio, during a 5-year observation period.MethodsSeventy-three patients with type 2 diabetes mellitus were randomly assigned to a drug therapy with pioglitazone or glimepiride, combined to metformin. Each subject was evaluated at baseline and after 5 years of treatment.ResultsIn both groups s-RAGE levels did not significantly vary, while the levels of AGE and AGE/s-RAGE were both significantly reduced, basal compared to 5-year values. Within pioglitazone group, as well within glimepiride group, significant variations (Δ, as difference between 5 years of treatment minus basal) were observed for AGE (Δ= ˗21.1±13.4 µg/ml, P&lt;0.001 for pioglitazone; Δ= ˗14.4±11.4 µg/ml, P&lt;0.001 for glimepiride) and in AGE/s-RAGE (Δ= -0.037±0.022 µg/pg, P&lt;0.001 for pioglitazone; Δ= -0.024±0.020µg/pg, P&lt;0.001 for glimepiride), suggesting an average decrease of the parameters by more than 50% in both treatments. Pioglitazone was more effective than glimepiride in reducing AGE/s-RAGE ratio after 5 years of therapy.ConclusionThese data can help to explain the benefits of oral anti-diabetic therapy in relation to the reduction of cardiovascular risk, as suggested by variations in AGE/s-RAGE ratio as biochemical marker of endothelial function; in particular, treatment with pioglitazone seems to offer greater long-term benefit on AGE-RAGE axis.
Development and validation of a Sarcopenia Geriatric Scale (SARCO-GS): a new short scale for the screening of sarcopenia
Authors: Rosas-Carrasco Oscar , Omaña-Guzmán Isabel , García-González Ana Isabel , Luna-López Armando 
Published: August 2023

IntroductionSarcopenia is a highly prevalent disease associated with adverse outcomes such as falls, disability, and death. The current international consensuses agree that muscle strength, muscle mass, and gait speed must be included in the definition. However, these proposed criteria require objective measurements that are not available for most populations. Since the timely identification of sarcopenia is a priority, several subjective screening scales have been developed; however, they have some limitations due to their low sensitivity. The objective of this work was to develop and validate SARCO-GS, a new short scale to screen sarcopenia that is affordable, easy, and accessible for all clinical care settings.Methods and materialsThe development of the SARCO-GS included four stages: (1) Review and analysis of documentary sources, (2) Contextualization of the theoretical model of sarcopenia, (3) Scale conformation, and (4) Reliability and validity analyses. SARCO-GS was validated in the FraDySMex study, which is a longitudinal cohort of community-dwelling adults.ResultsIn the studied population (n=852), the average age was 68.9 years (SD 10.21) and 80.1% of the participants were women. SARCO-GS is a seven-item scale with an innovative structure that included five subjective questions (gait speed, muscular strength, muscle mass) and two measurements of muscular strength and muscle mass (Chair stand test and calf circumference). The results regarding criterion validity showed that the cut-off point ≥ 3 had good sensitivity (77.68%) versus the EWGSOP2 consensus, with an adequate Area Under the Receiver Operating Characteristic (AUC) (0.73), in addition to showing higher values of sensitivity and AUC than SARC-F and SARC-CalF using as reference the same consensus. Furthermore, SARCO-GS presented good predictive validity for functional dependence (HR=2.22, p=0.046) and acceptable correlation with other related measurements (construct validity). Regarding reliability, the scale showed acceptable internal reliability (correlation between items and total score: 0.50 to 0.70). After the validation analysis, the scale was adapted to English.ConclusionsThe SARCO-GS is a novel scale to screen sarcopenia with high sensitivity, good construct, predictive validity, and internal reliability that may be useful for health professionals in different clinical settings and for clinical research.
Diagnosis of thyroid disease using deep convolutional neural network models applied to thyroid scintigraphy images: a multicenter study
Authors: Zhao Huayi , Zheng Chenxi , Zhang Huihui , Rao Maohua , Li Yixuan , Fang Danzhou , Huang Jiahui , Zhang Wenqian , Yuan Gengbiao 
Published: August 2023

ObjectivesThe aim of this study was to improve the diagnostic performance of nuclear medicine physicians using a deep convolutional neural network (DCNN) model and validate the results with two multicenter datasets for thyroid disease by analyzing clinical single-photon emission computed tomography (SPECT) image data.MethodsIn this multicenter retrospective study, 3194 SPECT thyroid images were collected for model training (n=2067), internal validation (n=514) and external validation (n=613). First, four pretrained DCNN models (AlexNet, ShuffleNetV2, MobileNetV3 and ResNet-34) for were tested multiple medical image classification of thyroid disease types (i.e., Graves’ disease, subacute thyroiditis, thyroid tumor and normal thyroid). The best performing model was then subjected to fivefold cross-validation to further assess its performance, and the diagnostic performance of this model was compared with that of junior and senior nuclear medicine physicians. Finally, class-specific attentional regions were visualized with attention heatmaps using gradient-weighted class activation mapping.ResultsEach of the four pretrained neural networks attained an overall accuracy of more than 0.85 for the classification of SPECT thyroid images. The improved ResNet-34 model performed best, with an accuracy of 0.944. For the internal validation set, the ResNet-34 model showed higher accuracy (p &lt; 0.001) when compared to that of the senior nuclear medicine physician, with an improvement of nearly 10%. Our model achieved an overall accuracy of 0.931 for the external dataset, a significantly higher accuracy than that of the senior physician (0.931 vs. 0.868, p &lt; 0.001).ConclusionThe DCNN-based model performed well in terms of diagnosing thyroid scintillation images. The DCNN model showed higher sensitivity and greater specificity in identifying Graves’ disease, subacute thyroiditis, and thyroid tumors compared to those of nuclear medicine physicians, illustrating the feasibility of deep learning models to improve the diagnostic efficiency for assisting clinicians.
Sex non-specific growth charts and potential clinical implications in the care of transgender youth
Authors: Bomberg Eric Morris , Miller Bradley Scott , Addo Oppong Yaw , Rogol Alan David , Jaber Mutaz M. , Sarafoglou Kyriakie 
Published: August 2023

IntroductionThe Centers for Disease Control and Prevention (CDC) and World Health Organization (WHO) created separate growth charts for girls and boys because growth patterns and rates differ between sexes. However, scenarios exist in which this dichotomizing “girls versus boys” approach may not be ideal, including the care of non-binary youth or transgender youth undergoing transitions consistent with their gender identity. There is therefore a need for growth charts that age smooth differences in pubertal timing between sexes to determine how youth are growing as “children” versus “girls or boys” (e.g., age- and sex-neutral, compared to age- and sex-specific, growth charts).MethodsEmploying similar statistical techniques and datasets used to create the CDC 2000 growth charts, we developed age-adjusted, sex non-specific growth charts for height, weight, and body mass index (BMI), and z-score calculators for these parameters. Specifically, these were created using anthropometric data from five US cross-sectional studies including National Health Examination Surveys II-III and National Health and Nutrition Examination Surveys I-III. To illustrate contemporary clinical practice, we overlaid our charts on CDC 2000 girls and boys growth charts.Results39,119 youth 2-20 years old (49.5% female; 66.7% non-Hispanic White; 21.7% non-Hispanic Black) were included in the development of our growth charts, reference ranges, and z-score calculators. Respective curves were largely superimposable through around 10 years of age after which, coinciding with pubertal onset timing, differences became more apparent.DiscussionWe conclude that age-adjusted, sex non-specific growth charts may be used in clinical situations such as transgender youth in which standard “girls versus boys” growth charts are not ideal. Until longitudinal auxological data are available in these populations, our growth charts may help to assess a transgender youth’s growth trajectory and weight classification, and expectations surrounding these.
Polycystic ovary syndrome and thyroid disorder: a comprehensive narrative review of the literature
Authors: Palomba Stefano , Colombo Carla , Busnelli Andrea , Caserta Donatella , Vitale Giovanni 
Published: August 2023

BackgroundPublished data on the relationship between polycystic ovary syndrome (PCOS) and thyroid dysfunction are sparse and confusing.ObjectiveTo comprehensively review data available in the literature regarding the relationship between PCOS and the thyroid function, and its abnormalities.MethodsNine main areas of interest were identified and analyzed according to the available evidence: 1) Evaluation of thyroid function for PCOS diagnosis; 2) Epidemiology data on thyroid function/disorders in patients with PCOS, and vice versa; 3) Experimental data supporting the relationship between thyroid function/disorders and PCOS; 4) Effects of thyroid function/disorders on PCOS features, and vice versa; 5) Effect of thyroid alterations on the cardiometabolic risk in women with PCOS; 6) Effect of thyroid abnormalities on reproductive outcomes in women with PCOS; 7) Relationship between thyroid function/abnormalities in patients with PCOS who are undergoing fertility treatment; 8) Effect of treatments for thyroid diseases on PCOS; and 9) Effect of treatments for PCOS on thyroid function. An extensive literature search for specific keywords was performed for articles published from 1970 to March 2023 using PubMed and Web of Science. Data were reported in a narrative fashion.ResultsPCOS is a diagnosis of exclusion for which diagnosis is possible only after excluding disorders that mimic the PCOS phenotype, including thyroid dysfunctions. However, the tests and the cutoff values used for this are not specified. Many experimental and clinical data suggest a relationship between perturbations of the thyroid function and PCOS. Direct and unequivocal evidence on the effects of thyroid function/disorders on PCOS features are lacking. High thyroid-stimulating hormone levels and subclinical hypothyroidism may be associated with significant worsening of several intermediate endpoints of cardiometabolic risk in women with PCOS. Thyroid abnormalities may worsen reproductive outcomes, especially in patients undergoing fertility treatment. To date, there are no data demonstrating the efficacy of thyroid medications on fertility and cardiometabolic risk in women with PCOS. Lifestyle modification changes, metformin, and vitamin D seem to improve thyroid function in the general population.ConclusionPCOS and thyroid disorders are closely related, and their coexistence may identify patients with a higher reproductive and metabolic risk. Regular screening for thyroid function and thyroid-specific autoantibodies in women with PCOS, particularly before and during pregnancy, is highly recommended.
Grassland management effect on ecosystem services in the livestock system in an oxisol from the Eastern high plains of Colombia
Authors: Rodríguez-Hernández Nubia S. , Arango Miguel , Moreno-Conn Lina M. , Arguello José Orlando , Bernal-Riobo Jaime H. , Pérez-López Otoniel 
Published: August 2023

Colombia has a livestock population of approximately 28.2 million heads, of which 20.4% is found in the eastern high plains of the Orinoquia region. The extensive beef cattle system predominates, whose diet is based on native and introduced pastures of the genus Urochloa sp. which is deficiently managed, affecting productive and reproductive indexes due to the low adoption of technology. We evaluated potential sustainable intensive systems for cattle production, which contribute to maintaining the provision of environmental services. Between 2011 and 2015 in Agrosavia’s Research Center at Carimagua (4° 37'N and 71° 19' W) environmental and animal production variables were monitored in the following systems: a) degraded pastures recovered with tillage and fertilization, b) annual crop rotation with pastures, c) forest arrangements in strips, and d) forest remnants in perimeter areas. Productive and reproductive variables were determined in animals such as weight gain, calving interval, among others while the pasture/crop productive variables included yield and forage quality. Regarding soil ecosystem services (ES) the macrofauna biodiversity, biogeochemical cycles, soil physical and chemical variables were considered. Estimation of indicators was carried out through principal components analysis for soil physical, chemical and macrofauna variables to extract the two main components that explain the variance. For climate regulation of ES, measurement of soil organic carbon (SOC) storage at a depth of 20 cm and the annual accumulated greenhouse gas (GHG) emissions were included. The systems that involved lime amendments and fertilizers increased the value in the year of application. Values in the water regulation indicator did not show significant differences among the options implemented during the years. Edaphic macrofauna biodiversity indicator value was sensitive to changes in management practices, with termites being the group with highest abundance. The indicator related to SOC was higher in the forest area compared to the pasture-crop system. Systems under the agroforestry schemes which integrated various practices such as managed and recovered pastures, crop-pasture rotation, patched areas and tree strips contributed to maintain or improve the ES, although each one of the strategies proposed provided improvement in at leas one ES.
The complex HLA-E-nonapeptide in Behçet disease
Authors: Castaño-Núñez Ángel Luís , Montes-Cano Marco-Antonio , García-Lozano José-Raúl , Ortego-Centeno Norberto , García-Hernández Francisco José , Espinosa Gerard , Graña-Gil Genaro , Sánchez-Bursón Juan , Juliá María Rosa , Solans Roser , Blanco Ricardo , Barnosi-Marín Ana-Celia , Gómez de la Torre Ricardo , Fanlo Patricia , Rodríguez-Carballeira Mónica , Rodríguez-Rodríguez Luis , Camps Teresa , Castañeda Santos , Alegre-Sancho Juan-Jose , Martín Javier , González-Escribano María Francisca 
Published: August 2023

IntroductionThe knowledge of the aetiology of Behçet disease (BD), an immune-mediated vasculitis, is limited. HLA-B, mainly HLA-B51, and HLA-A molecules are associated with disease, but the ultimate cause of this association remains obscure. There is evidence that NK cells participate in the etiopathology of BD. NK cells have activator and inhibitor surface receptors, like the KIR and the NKG2 families. Classical HLA-class I molecules (A, B and C) are keys in the activity control of the NK because they are KIR ligands. Most NKG2 receptors bind HLA-E, which presents only nonapeptides derived from the signal peptide of other class-I molecules.ObjectiveThis study investigates the contribution of the pair HLA-E and ligand, nonapeptide derived from the 3-11 sequence of the signal peptides of class I classical molecules, to the susceptibility to BD.MethodsWe analyzed the frequency of the HLA-derivated nonapeptide forms in 466 BD patients and 444 controls and an HLA-E functional dimorphism in a subgroup of patients and controls. Results: In B51 negative patients, the frequency of VMAPRTLLL was lower (70.4% versus 80.0% in controls; P=0.006, Pc=0.04, OR=0.60, 95%CI 0.41-0.86), and the frequency of VMAPRTLVL was higher (81.6% versus 71.4% in controls; P=0.004, Pc=0.03, OR=1.78, 95%CI 1.20-2.63). In homozygosity, VMAPRTLLL is protective, and VMAPRTLVL confers risk. The heterozygous condition is neutral. There were no significant differences in the distribution of the HLA-E dimorphism.DiscussionOur results explain the association of BD with diverse HLA-A molecules, reinforce the hypothesis of the involvement of the NK cells in the disease and do not suggest a significant contribution of the HLA-E polymorphism to disease susceptibility.
Characterization of papillary and clear cell renal cell carcinoma through imaging mass cytometry reveals distinct immunologic profiles
Authors: Govindarajan Ameish , Salgia Nicholas J. , Li Haiqing , Castro Daniela V. , Mirzapoiazova Tamara , Armstrong Brian , Zhao Dan , Mercier Benjamin D. , Dizman Nazli , Chawla Neal , Zengin Zeynep , Meza Luis , Tripathi Nishita , Sayegh Nicolas , Chehrazi-Raffle Alex , Tripathi Abhishek , Pal Sumanta K. 
Published: August 2023

ObjectiveTo characterize and further compare the immune cell populations of the tumor microenvironment (TME) in both clear cell and papillary renal cell carcinoma (RCC) using heavy metal-labeled antibodies in a multiplexed imaging approach (imaging mass cytometry).Materials and methodsFormalin-fixed paraffin-embedded (FFPE) baseline tumor tissues from metastatic patients with clear cell renal cell carcinoma (ccRCC) and papillary renal cell carcinoma (pRCC) were retrospectively requisitioned from an institutional biorepository. Pretreated FFPE samples from 33 RCC patients (10 ccRCC, 23 pRCC) were accessioned and stained for imaging mass cytometry (IMC) analysis. Clinical characteristics were curated from an institutional RCC database. FFPE samples were prepared and stained with heavy metal-conjugated antibodies for IMC. An 11-marker panel of tumor stromal and immune markers was used to assess and quantify cellular relationships in TME compartments. To validate our time-of-flight (CyTOF) analysis, we cross-validated findings with The Cancer Genome Atlas Program (TCGA) analysis and utilized the CIBERSORTx tool to examine the abundance of main immune cell types in pRCC and ccRCC patients.ResultsPatients with ccRCC had a longer median overall survival than did those with pRCC (67.7 vs 26.8 mo, respectively). Significant differences were identified in the proportion of CD4+ T cells between disease subtypes (ccRCC 14.1%, pRCC 7.0%, p&lt;0.01). Further, the pRCC cohort had significantly more PanCK+ tumor cells than did the ccRCC cohort (24.3% vs 9.5%, respectively, p&lt;0.01). There were no significant differences in macrophage composition (CD68+) between cohorts. Our results demonstrated a significant correlation between the CyTOF and TCGA analyses, specifically validating that ccRCC patients exhibit higher levels of CD4+ T cells (ccRCC 17.60%, pRCC 15.7%, p&lt;0.01) and CD8+ T cells (ccRCC 17.83%, pRCC 11.15%, p&lt;0.01). The limitation of our CyTOF analysis was the large proportion of cells that were deemed non-characterizable.ConclusionsOur findings emphasize the need to investigate the TME in distinct RCC histological subtypes. We observed a more immune infiltrative phenotype in the TME of the ccRCC cohort than in the pRCC cohort, where a tumor-rich phenotype was noted. As practical predictive biomarkers remain elusive across all subtypes of RCC, further studies are warranted to analyze the biomarker potential of such TME classifications.
Uncovering the shared molecule and mechanism between ulcerative colitis and atherosclerosis: an integrative genomic analysis
Authors: Huang Jinke , Wang Fengyun , Tang Xudong 
Published: August 2023

BackgroundUlcerative colitis (UC) and atherosclerosis (AS) are closely related. However, the pathologic mechanisms underlying the co-occurrence of UC and AS are not well understood.ObjectsTo reveal the hub molecule and mechanism involved in the co-occurrence of UC and AS.MethodsDifferentially expressed genes (DEGs) of UC and AS were obtained, and the shared DEGs of UC and AS were explored for biological function. Next, the hub genes were explored using the cytoHubba plugin. The predictive ability of the hub genes was measured by constructing the receiver operating characteristic curve. Analyses of immune infiltration and the single-gene gene set enrichment analysis (GSEA) for the hub genes were further carried out.ResultsIdentification of 59 DEGs (55 were upregulated and four were downregulated) shared by both UC and AS was performed. Enriched pathways of the shared DEGs were mainly related to immunity and inflammation. Protein tyrosine phosphatase, receptor type, C (PTPRC) was identified as the hub crosstalk gene for the comorbidity of UC and AS. The upregulation of PTPRC was correlated with mast cells resting, T cells CD4 memory resting, macrophages M0, and macrophages M1. Pathways of immune and inflammatory processes, including NF-kappa B, viral protein interaction with cytokine and cytokine receptor, and cytokine–cytokine receptor interaction, were significantly correlated with high expression of PTPRC in UC and AS.ConclusionAt the transcriptional level, our study reveals that imbalanced inflammatory and immune responses are the key pathological mechanisms underlying the comorbidity of UC and AS and that PTPRC is a key biomarker for the comorbidity of UC and AS.