bluuebunny/crossref_metadata_2025_split huggingface dataset:
การศึกษาการตรวจคัดกรองโรคโกเชร์อย่างเป็นระบบในผู้ป่วยที่มีม้ามโต และ/หรือ เกล็ดเลือดต่ำ
Authors:   , ชื่นกลิ่น ปิยะพร 
Published: September 2023

ที่มาและความสำคัญ: โรคโกเชร์ (Gaucher disease) เป็นโรคที่เกิดจากความผิดปกติทางพันธุกรรม ของยีนเบต้ากลูโคสิเดส ผู้ป่วยที่เป็นผู้ใหญ่มักจะมาด้วยม้ามโต และ/หรือเกล็ดเลือดต่ำ แต่จากการที่เป็นโรคที่พบได้น้อยมาก การวินิจฉัยโรคโกเชร์จึงอาจจะพลาดไป ถ้าไม่ได้มีการตรวจคัดกรองอย่างเป็นระบบ การศึกษาจากประเทศอิตาลีพบความชุกร้อยละ 3.6 ในผู้ป่วย 196 รายที่มาด้วยม้ามโตและ/หรือเกล็ดเลือดต่ำ อย่างไรก็ตาม ความชุกของโรคโกเชร์ในประเทศไทยยังไม่มีการศึกษาอย่างชัดเจน

วิธีการ : ศึกษาวิเคราะห์แบบตัดขวาง (cross-sectional study) เพื่อประเมินความชุกของโรคโกเชร์ในผู้ป่วยที่มีม้ามโต และ/หรือ เกล็ดเลือดต่ำ โดยใช้การตรวจคัดกรองอย่างเป็นระบบ ด้วยวิธี dry blood spot enzyme assay (DBS) และตรวจยืนยันผลด้วยการตรวจเอ็นไซม์และยีน ในกรณีที่ผลการตรวจคัดกรองเป็นบวก นอกจากนี้ยังศึกษาลักษณะต่างๆทางคลินิกที่ช่วยในการบ่งชี้การวินิจฉัยสุดท้ายแต่ละอย่าง

ผลการศึกษา : มีผู้ป่วยที่มาด้วยม้ามโต และ/หรือเกล็ดเลือดต่ำ จำนวนทั้งสิ้น 195 ราย เข้าร่วมในงานวิจัย อายุเฉลี่ยเท่ากับ 49.5 ปี (15-91 ปี) เป็นเพศหญิง 122 ราย (ร้อยละ 62.5) ผู้ป่วย 26 ราย มีม้ามโตและเกล็ดเลือดต่ำ โดยในกลุ่มนี้วินิจฉัยว่าเป็นตับแข็งจำนวน 14 ราย (ร้อยละ 53.8) วินิจฉัยว่าเป็นธาลัสซีเมีย 6 ราย (ร้อยละ 23) และเป็นกลุ่มโรคมะเร็งทางโลหิต 3 ราย (ร้อยละ 11.5) ผู้ป่วยจำนวน 56 รายมีม้ามโต โดยไม่มีเกล็ดเลือดต่ำ พบเป็นธาลัสซีเมียร้อยละ 71.4 พบเป็นมะเร็งทางโลหิต ร้อยละ 21.4 และ ตับแข็ง ร้อยละ 1.79 กลุ่มที่มีเกล็ดเลือดต่ำมีจำนวน 113 ราย โดยการวินิจฉัยที่พบมากที่สุดคือ immune thrombocytopenia (ITP) พบร้อยละ 51.3 วินิจฉัย myelodysplastic syndrome (MDS) ร้อยละ 17 กลุ่มมะเร็งโลหิตอื่นๆ และตับแข็ง อย่างละร้อยละ 7 จากผู้ป่วย 195 ราย มีผู้ป่วย 2 รายที่ตรวจพบมีเบต้ากลูโคสิเดสเอนไซม์ต่ำจาก DBS โดยเป็นผู้ป่วยที่มีม้ามโตและเกล็ดเลือดต่ำ แต่จากการตรวจยืนยันผลไม่พบความผิดปกติ โดยผู้ป่วยทั้งสองราย วินิจฉัยสุดท้ายเป็นตับแข็ง ในด้านการบ่งชี้การวินิจฉัยสุดท้าย โรคธาลัสซีเมียพบ ค่า Hb 10 ก/ดล โดยมี ความไว ร้อยละ 89.1 ความจำเพาะร้อยละ 71.1 ค่า MCV 80 fL โดยมีความไวร้อยละ 87 ความจำเพาะร้อยละ 81.9 ค่า RDW 20% โดยมีความไวร้อยละ 80.4 ความจำเพาะร้อยละ 91.3 และ ferritin ที่ 800 mcg/L โดยมีความไวร้อยละ 80 ความจำเพาะร้อยละ 80.8 นอกจากนี้ค่า albumin 3.5 ก/ดล สัมพันธ์กับโรคตับแข็ง โดยมี Odds ratio 5.32 (95% confidence interval 2.13 - 13.3)

สรุป : ความชุกของโรคโกเชร์ในประเทศไทย (0/195) อาจต่ำกว่าที่เคยมีการศึกษาในยุโรป นอกจากนี้พบว่าค่า Hemoglobin ดัชนีเม็ดเลือดแดง albumin และ ferritin มีประโยชน์ในการวินิจฉัยผู้ป่วยไทยที่มีม้ามโต และเกล็ดเลือดต่ำ
An affordable immunohistochemical approach to estimate the prevalence of BRAF V600E mutation in papillary thyroid cancer patients of King Chulalongkorn Memorial Hospital, Thailand.
Authors:   , Choden Sonam 
Published: September 2023

Background: Papillary thyroid carcinoma (PTC) accounts for the majority of diagnoses of thyroid carcinoma. BRAFV600E mutation is the most common genetic alteration in PTC, which has diagnostic and prognostic significance. The rate of BRAFV600E mutation in PTC from Thailand has not been reported. Our purpose was to estimate the prevalence of BRAF mutation in a large institutional series using an affordable approach, which combined mutation-specific immunohistochemistry (IHC) with VE1 antibody and tissue microarray (TMA).

Methods: A total of 476 PTC cases plotted on TMA were employed for determining the mutation status in this study. The cancer tissue of initial 100 cases (pilot study) were analyzed for BRAFV600E mutation by using both direct sequencing and VE1 immunostaining. For the subsequent PTC cases, VE1 IHC was used as an alternative to direct sequencing for the detection of mutation. Univariate and multivariate analyses were done to determine the association of clinicopathological variables with BRAFV600E mutation.

Results: In the pilot study, VE1 IHC showed excellent analytical performance (κ = 0.884) for detecting BRAFV600E mutation in PTC TMA as compared to direct sequencing. The prevalence of BRAFV600E in the whole cohort was 60.9% by using VE1 IHC. The mutation was commonly seen in tall cell (92.9%) and classic (70.2%) variants of PTC. Multivariate analysis (P<0.05) showed association of BRAFV600E with histological type of tumor, extrathyroidal extension, and absence of Hashimoto’s thyroiditis.

Conclusions: In conclusion, BRAFV600E mutation was detected in 60.9% of Thai PTC and it was associated with several aggressive clinicopathological variables of thyroid cancer. VE1 IHC proved as a reliable method able to replace direct sequencing for the detection of the mutation. A combination of mutation-specific IHC and TMA allows conducting large cohort studies more labor-saving and cost-efficiently.
Wage compensation differentials for job risk between formal and informal workers
Authors:   , Suwannarat Pinyada 
Published: September 2023

This research estimates compensating wage differentials for job risks for formal and informal workers in Thailand, the latter of whom are not covered by prevailing labor laws. Using data from the National Statistical Office’s Thai Labor Force Survey for years 2012 to 2018 merged with job fatality risk collected by the Social Security Office, the results show that the fatality risk rate has a positive, significant effect on wages. The value of statistical life for the median formal workers is 79.33 million baht compared to 41.5 million baht for the median informal worker. Also, we analyze the relationship between self-reported unsafe work conditions in the Labor Force Survey with wages. The results showed that the coefficient on safety issues for formal workers is positive, while the coefficient for informal workers is negative for both OLS and quantile regression in all wage distribution. All the results show that there is inequality in access to labor protection and the ability to negotiate with employers. This results in the compensation wage differentials for formal and informal workers.
Analyzing the impact of biofuel industry on Thailand’s
economy based on input-output model
Authors:   , Limchaikit Thipruedee 
Published: September 2023

This research tries to examine the impact of the biofuel industry focusing on ethanol and biodiesel on Thailand's economy. It uses input-output analysis. In the past 10 years, Thailand's Alternate Energy Development Plan (AEDP) has played a significant role in launching policies and promoting the biofuel industry. The plan focuses on increasing internal energy security by increasing the use of alternate sources of energy which is a perfect replacement for the importation of fuels. However, the main challenge is price competitiveness which requires subsidization to sustain its market in the industry. Therefore, the main objective of this study is to focus on the impacts of biofuels on every sector and the total output of the Thailand economy. The biofuel industry can increase the household income and the total output of the Thailand economy. However, the government might slightly loose on value-added tax but that benefit is channeled to household income. This calls for collective efforts in promoting the biofuel industry.
Policy for sustainable informal transport - a case study of feeder services in Bangkok, Thailand
Authors:   , Amrapala Chutaporn 
Published: September 2023

One informal public transport service in Bangkok is Silor (SR), given the meaning in Thai as four-wheeler. SR facilitates urban mobility both in terms of major travel mode and feeder bus and mass transit lines in the city. This research aims to investigate service characteristics and challenges of SR service, identify factors affecting the use and non-use of SR, explore travel behavior and attitudes to determine service delivery gaps in order to propose policy recommendations for the better functioning of SR service. Interviews are conducted through questionnaire survey to collect data from supply side, including drivers and regulators, and demand sides, which involves both users and non-users. Five SR routes are selected for study namely, Bangbon-Taladplu, Siriraj-Taladplu, Charansanitwong-Klongsan, Vibhavadi-Rachadapisek, and Sukhumvit Soi 39. Findings from supply side reveal challenges in terms of work hour, working condition, registration of vehicles and drivers, experiences when called by polices as well as competitions among transport modes. For demand side, reasons for using SR are convenience, accessibility, and cheap fare whereas difficulties for non-users seem to associate with connection, transfer and crowdedness. Importance-performance analysis are performed with users’ attitudes on service quality and found that reliability, connection and transfer, seat availability and fare are the aspects of high importance and highly satisfied while safety and security seem to be of high importance but low satisfaction. In addition, exploratory factor analysis and logistic regression model illustrate the four service factors that significantly affect the overall satisfaction of users, including reliability, in-vehicle environment, comfort and convenience, and environmental impact. Further, the study applies user segmentation through cluster analysis based on the obtained factors which results in four user subgroups having diverse profiles and expectations. Moreover, travel behavior and perceptions of Thai and Japanese SR users are comparatively analyzed and considerable variations among them are found. For sustainability aspects, travelling by SR is more desirable than alternative modes in terms of less commute time, more affordable, less energy consumption and emissions; however, there still need more attention on provision of affordable, reliable, comfort and convenient services to users of all socioeconomic groups. Most feasible options for future policy suggest transport authorities and relevant sectors in formalizing and integrating SR into urban transportation network in order to provide society with efficient alternative mode for a sustainable means of travelling.
Enhancement of municipal wastewater management system with polluter pays principle (PPP): a case study of cities in Thailand
Authors:   , Meethavorn Kwanmanas 
Published: September 2023

Poor water quality in public water bodies caused by several reasons mainly due to a lack of efficient WWM system and sufficient financial support. Polluter pays principle has long been a promising strategy to help improving water quality in pubic water bodies in Thailand. However, WW charge has not yet been practically levied. This research ains to evaluate factors affecting residents' preferences on WTPs for water quality improvement. Three different characteristic cities were selected as case studies. The technique applied to estimate WTPs is CVM to reveal key factors influencing WTP decision as well as WTP pay-out level. The result has evidenced that cities with different characteristics have factors influencing WTPs differently. However, a common factor of all city is, ones who perceive that their houses are located in the WW service area, are more likely to pay for WW charge in the higher amount than the others who do not reside within service area or uncertain whether their house are covered in the service area. As a case study, Pattaya (coastal city) has the highest rate of WW charge pay-out level followed by Bangkok (urbanised city) and Tha Rae (rural city) respectively. Key factors influencing WTPs as well as range of WTP for WW charge identified in this research could help supporting strategic planners to design suitably pragmatic WWM schemes and approaches for enhancing sustainable WWM in different context cities in Thailand and other countries.
Evaluation of carbon dioxide and nitrate utilization from recirculating aquaculture system for microalgal production
Authors:   , Sucunthowong Kittikoon 
Published: September 2023

This study evaluated carbon dioxide and nitrate utilization from tilapia cultivating system by means of microalgal cultivation. The first part of this study evaluated the feasibility of using nitrate-rich effluent from aquaculture to cultivate Scenedesmus armatus in comparison with the cultivation using BG-11 media. Comparable biomass productivity and pigment content of S. armatus from the cultivation indicated that nitrate-rich effluent from aquaculture could be used as substitute for a more expensive BG-11 growth media. The second part of this study evaluated the feasibility of utilizing carbon dioxide concentrated air from roughly 3 kg/m3 tilapia cultured tank to grow S. armatus. It was found that the maximum productivity of S. armatus at 110 mg/L·day was associated with using BG-11 media and carbon dioxide from fish tank although biomass dried weights and maximum specific growth rates were insignificant different as compared to other treatments using ambient air and BG-11 media without Na2CO3. The final part of the study examined the effect of fish biomass on carbon dioxide and nitrate production and their utilization by means of microalgal cultivation. Approximately 3, 5 and 10 kg/m3 of fish (tilapia) biomass were introduced into the recirculating aquaculture system and cultivated for 8 days and measured for carbon dioxide and nitrate production. Nitrate concentrations were relatively constant at 45.3 mg N/L for the range of fish biomass considered. The highest carbon dioxide production was found in 10 kg/m3 fish cultivation, measured at 1,605 and 915 ppm from outlet of fish tank and solid separating unit, respectively. Outlet air from this particular fish cultivation (i.e., 10 kg/m3) also yielded significantly higher biomass of S. armatus as compared to those using ambient air. Carbon and nitrogen mass balance calculation from tilapia cultivation indicated that 7.99% to 16.42% of carbon and 7.58% to 15.58% of nitrogen from feed were accounted in fish biomass while significantly larger fraction of carbon in feed from 50.67% to 73.46% was converted to carbon dioxide. Only 2.2% to 3.4% of carbon dioxide produced were accounted into the biomass of S. armatus, whereas 33.40% to 74.63% of input nitrogen, mostly nitrate, from solid separating unit were transferred into microalgal biomass. The results from this work clearly demonstrated that microalgal cultivation was able to utilize wastes, namely carbon dioxide and nitrate, from recirculating aquaculture system to produce valuable products, and moreover, reduced adverse environmental impacts. In the broader picture, this work followed closely the concept of bio-, circular- and green (i.e., BCG) economy, which is one of the flagship policies of Thai government to propel the country from middle-income trapped status.
Effects of astaxanthin supplementation on biological parameters in Thai healthy volunteers
Authors:   , Sratongfaeng Chuenjai 
Published: September 2023

Astaxanthin, a powerful antioxidant, is widely used as a dietary supplement to promote several health benefits. However, the effects of long-term supplementation on general biological parameters in healthy adults is still insufficient. The objective of this study was to investigate the effects of 12-week astaxanthin supplementation on blood glucose, lipid profile and hematological parameters in healthy volunteers. Thirty-three healthy participants (16 in the experimental group and 17 in the placebo group) were enrolled in the study. The participants in the experimental group were supplemented with 4 mg/day of astaxanthin while those in the placebo group were supplemented with soybean oil capsule for 12 weeks. Body mass index and blood pressure were recorded, and blood sample was collected to determine serum astaxanthin, fasting blood glucose (FBG), lipid parameters, and hematological parameters before and after the supplementation. Moreover, the daily dietary intake was assessed by 3-day food record, and adverse effects during supplementation were investigated. After astaxanthin supplementation, serum astaxanthin concentration significantly increased from baseline (p < 0.05), and significantly 5-fold higher than those in the placebo group (p < 0.05). There were no significant changes in FBG, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol from baseline after 12-week supplementation in both groups. Although significant increased triglyceride level was found in the experimental group at week 12 (p < 0.05), the level was still in the reference range. No differences in FBG and lipid parameters at week 12 between groups were observed. In this study, most of hematological parameters were not affected by astaxanthin supplementation. Interestingly, the results showed reduced total energy and carbohydrate intakes of the participants in the experimental group at week 12 (p < 0.05), and their sodium intake was significantly lower than those in the placebo group (p < 0.05). No serious adverse effects were reported. Red stool, mild itching and loosing appetite occurred and then became better without discontinuing the supplementation. The findings suggested no effects on general biological parameters and potential dietary intake-lowering effect of astaxanthin in healthy individuals.
Geoinformatics and predictive model for malaria risk in Thailand
Authors:   , Krainara Patcharaporn 
Published: September 2023

Malaria is an infectious disease caused by the parasite Plasmodium spp., and transmitted by an anopheles mosquito as a vector. After an infected female Anopheles mosquito bites a person, a parasite from salivary glands is delivered into the human’s blood flow. The incubation period of the disease is between 10-14 days and in the severe cases, it can cause jaundice, seizures, coma, or death. Many researches indicated that the occurrence of malaria requires many factors, including environmental factors, landscape and climate. Therefore, every healthcare organization delivered policies to control these typical factors towards human’s living styles. Though malaria has been cleared for decades, there is one serious problem that there were emergences of malaria parasite that resisted various types of medicines in use. So, it causes the complication in malaria control processes. To solve such complication, this study collected data mainly from the National Statistical Office database and applied statistical method, combining with the machine learning technique and Geographic Information System to determine risk factors, risk model, and risk map for malaria distribution in Thailand. As a result of these methods, this study proposed a new set of monitoring parameters, 28 parameters, a logistic model tree (LMT) risk model, and a risk map for malaria distribution derived by the machine learning mechanism. The precision and recall of this tree are 0.780 and 0.821, respectively, which are higher than using the risk model created from the typical factors. As a result of this finding, the government can utilize these newly identified factors to develop suitable strategies to control malaria considering proper criteria. For example, the government should control the quality of rivers or even increase the amount of river monitoring in some areas to reduce the malaria risk. Once these factors are completely managed with well-developed plans, each community can reduce the risk of malaria without concern regarding drug resistance. Moreover, according to the risk map, each area in Thailand has been defined as high, medium, low distribution of malaria based on the discovery factors. In such result, the government can set suitable the malaria monitoring and protection strategies for each individual area with a proper budget to be provided.
Adsorption of haloacetonitriles by carbonized metal-organic frameworks
Authors:   , Rodbutr Phattarawarin 
Published: September 2023

This research investigated adsorption efficiency of four including; Chloroacetonitrile (MCAN), Dichloriacetonitrile (DCAN), Bromoacetonitrile (MBAN) and Dibromoacetonitrle (DBAN) on five acid-washed carbonized metal-organic frameworks (MOFs) (WC-ZIF-8, WC-MIL-53, WC-MIL-88B, WC-UiO-66 and WC-HKUST-1) compared with powder activated carbon (PAC S10). The synthesized materials were characterized by XRD, SEM-EDS, nitrogen adsorption isotherms, FTIR and XPS. The screening experiment revealed that WC-MIL-88B, WC-ZIF-8, and PAC had highest adsorption capacities for four HANs, respectively. The HANs molecular size and their hydrophobicity influenced the adsorption rates of HANs in the order of DBAN>DCAN>MBAN>MCAN. The adsorption isotherms showed that Di-halogenic HANs (DBAN and DCAN) can be adsorbed by WC-MOFs much better than mono-halogenic HANs (MBAN and MCAN). From XRD analysis, O-C=O and Fe-O functional groups of WC-MIL-88B might play the important role for adsorption of four HANs., adsorption sites of WC-MIL-88B are expected to be enough for mixed-solute HANs adsorption in low concentration range (0-100ppb), and the order of adsorption capacities does not change from the single-solute adsorption.
Increasing levodopa and carbidopa loading quantity in oral pullulan thin film
Authors:   , Krisai Kitisak 
Published: September 2023

Due to the non-compliance of Parkinson’s patient, both youth and elderly patients commonly suffer from the consequent of tablet administration. Therefore, the way to solve this patient’s non-compliance is the development of levodopa (LD) and carbidopa (CD) as an oral thin film (OTF) formulation. The current study focuses on the increasing solubility of LD and CD in order to improve the loading quantity for OTF preparation including formulation development and short-term stability investigation. The technique of solubilization by ionization approach was selected. Quantitative determination of solubilized LD and CD in various acid solvents showed the highest solubility of both drugs in 0.1 M HCl acid/0.1 M citric acid pH 1.5. The utilization of cosolvency, surfactant and high polymer concentration were also applied. The result revealed that they were not provide synergistic effect on the improving of LD and CD solubility in acid solvent. OTFs containing LD and CD were prepared by solvent casting method using pullulan as polymeric material. Glycerin and ascorbic acid were used as a plasticizer and antioxidant, respectively. The OTF formulation was assessed according to various physico-mechanical properties. The OTF produced without glycerin was breakable whereas 5% and 10%w/w addition provided tackier film. However, small level of glycerin at 1% and 2%w/w were successfully applied to gain appropriate OTF. Although high pullulan content of 8 %w/w was remarkably showed acceptable film characteristic, loading content of both drugs was negligibly improved. Therefore, the developed OTFs that met the requirements was found to be composed of 8 %w/w of pullulan with either glycerin added of 1% or 2 %w/w of dry pullulan. When considering the stability of mentioned products, they were not exhibited appreciable stability as shown with the significant disappearing of LD and CD content during storage. Further formulation development should be conducted by pointing out with specific additives and method of preparation.