bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recurrence monitoring using ctDNA in patients with metastasis of colorectal cancer: COSMOS- oligo study
Authors: Oki Eiji , Nakanishi Ryota , Ando Koji , Nambara Sho , Takemasa Ichiro , Watanabe Jun , Matsuhashi Nobuhisa , Kato Takeshi , Kagawa Yoshinori , Kotaka Masahito , Hirata Keiji , Sugiyama Masahiko , Kusumoto Tetsuya , Miyamoto Yuji , Toyosaki Kayo , Kishimoto Junji , Kimura Yasue , Yoshizumi Tomoharu , Nakamura Yoshiaki 
Published: May 2023

Abstract
The best treatment strategy for resectable metastatic colorectal cancer is surgical resection of the metastatic site. However, approximately 60% of patients show recurrence after the resection of metastatic lesions, and some patients require aggressive perioperative chemotherapy. We initiated new trials to evaluate the clinical benefits of circulating tumor DNA analysis and refine precision adjuvant therapy for resectable metastatic colorectal cancer, named COSMOS-oligo trials, including two studies. The COSMOS-CRC03 study is a prospective observational study to monitor circulating tumor DNA in patients with metastatic colorectal cancer who can undergo complete surgical resection. The AURORA trial is a randomized Phase II study designed to test whether postoperative mFOLFOXIRI plus bevacizumab is superior to the standard therapy with FOLFOX6 for 6 months in patients with metastatic colorectal cancer if the circulating tumor DNA status is positive at week 4 after curative surgery in the COSMOS-CRC03 study. In these studies, only patients with resectable distant metastases of colorectal cancer will be included. The study will examine the negative predictive value of circulating tumor DNA for recurrence, whether stratification using 28-day postoperative circulating tumor DNA results can select a population with a good prognosis, and whether circulating tumor DNA testing every 12 weeks will detect recurrence earlier than diagnostic imaging. Further, the Phase II trial will determine whether intensive treatment of circulating tumor DNA-positive cases can reduce recurrence. Stage IV colorectal cancer has no standard perioperative treatment. We designed this study to stratify patients using circulating tumor DNA and determine the optimal treatment. COSMOS-CRC03(jRCT2072220055); AURORA trial(jRCT1071220087)
AI-based drone system for medical support in congested areas
Authors: Kumar Chandrashekhar , T. Muthumanickam 
Published: May 2023

Abstract
Serving the needs of human beings is much more important to us. So, considering how to service the product or provide medicinal support is also very important to reach the destination. While supported by the local field-side forces, it is going through delays and getting affected by the delays in medical treatment. Why this failure in medical support? To avoid this delay, I have come up with a trend in technology in real life to send things like oxygen to the nearby hospital and make it emergency support on an urgent basis. At the same time, we can look for college and industry, food, and grocery items that we can utilize for the same methods. We can make it using AI technology used in the drone system and so by using different scales of sensors and cameras for recognition for bill service for auto-detection and bill payment as well. Nowadays, most people try to use their outside food purchases quickly, so we can get it via air drone easily without delay. This process is unintentionally free and GPS-based, with an advantage system to track the location. And confirmation of customer detection can help to unlock the lock-unlock the drone's locked body, get material, and lock the door once service is received. If there is any unwanted attack, it will be updated with the tracking system. There is an anti-drone system used here. This activity can be avoided.
The COP9 signalosome reduces neuroinflammation and attenuates ischemic neuronal stress in organotypic brain slice culture model
Authors: Tian Yuan , Milic Jelena , Monasor Laura Sebastián , Chakraborty Rahul , Wang Sijia , Yuan Yue , Asare Yaw , Behrends Christian , Tahirovic Sabina , Bernhagen Jürgen 
Published: May 2023

Abstract
The constitutive photomorphogenesis 9 (COP9) signalosome (CSN) is a deNEDDylase controlling ubiquitination activity of cullin-RING-E3 ligases (CRLs) and thus the levels of key cellular proteins. While the CSN and its catalytic subunit CSN5 have been extensively studied in cancer, its role in inflammatory and neurological diseases is less understood. Following verification that CSN5 is expressed in mouse and human brain, here we studied the role of the CSN in neuroinflammation and ischemic neuronal damage employing models of relevant brain-resident cell types, an ex vivo organotypic brain slice culture model, and the CRL NEDDylation state-modifying drugs MLN4924 and CSN5i-3, which mimic and inhibit, respectively, CSN5 deNEDDylase activity. Unbiased mass spectrometry-based proteomics revealed that MLN4924 and CSN5i-3 substantially alter the microglial proteome, including inflammation-related proteins. Applying these drugs, mimicking microglial and endothelial inflammation as well as ischemic neuronal stress by TNF-α and oxygen-glucose-deprivation/reoxygenation (OGD­/RO) treatment, we could link CSN5/CSN-mediated cullin deNEDDylation to reduction of microglial inflammation, attenuated cerebral endothelial inflammation, improved barrier integrity, as well as protection from ischemia stress-induced neuronal cell death. Specifically, MLN4924 reduced phagocytic activity, motility, and inflammatory cytokine expression of BV2 and primary microglial cells, and this was linked to inhibition of inflammation-induced NF-kB, MAPK, and Akt signaling. Inversely, Csn5 knockdown and CSN5i-3 increased NF-kB signaling. Moreover, MLN4924 abrogated TNF-a-induced NF-kB and MAPK signaling in cerebral microvascular endothelial cells (hCMECs) and rescued hCMEC monolayers from OGD/RO-triggered barrier leakage via restoring tight junctions, while CSN5i-3 exacerbated permeability. In an ex vivo organotypic brain slice model of ischemia/reperfusion stress, MLN4924 protected from neuronal death, while CSN5i-3 impaired neuronal survival. Neuronal damage was attributable to microglial activation and inflammatory cytokines, as indicated by microglial shape tracking and TNF-a-blocking experiments. Our results indicate a protective role of the CSN in neuroinflammation via several brain-resident cell types involved in ischemic brain disease and implicate CSN activity-mimicking deNEDDylating drugs as potential therapeutics.
Effect of the oxidation potential of shielding gas on Cr (VI) generation during Metal Inert/ Active Gas Welding (MIG/ MAG) and Flux Cored Arc Welding (FCAW) processes
Authors: Vats Vishal , Melton Geoff , Islam Meez , Krishnan Venkatesan Venkata 
Published: May 2023

Abstract
In this work, the role played by shielding gases in welding environments on carcinogenic Cr(VI) fume formation, is assessed. Shielding gases are characterized by oxidation index (OI), which is a crucial parameter likely to influence Cr(VI) formation in arc welding. The study found that the behavior of shielding gases towards Cr(VI) production differs between Flux Cored Arc Welding (FCAW) and Solid wire welding (MIG/ MAG), with the OI of the shielding gas playing a much more significant role for solid wire welding. The study also found that arc stability and ionization potential, induced by the choice of the shielding gas, influence the amount of Cr(VI) produced, as well. The use of a mixture of CO2 and O2 resulted in the least amount of Cr(VI) formation for the solid wire welding. In Flux cored wires (FCW) welding, the highest amount of Cr(VI) was observed when using Argon as the shielding gas due to the presence of Na and K in the wires, which promote the oxidation of Cr(III) to Cr(VI). The use of oxidizing shielding gases (higher values of OI) reduces the amount of Cr(VI) formation as Na and K react with oxygen to form their oxides, reducing the tendency to form chromates and dichromates, which are the most significant Cr(VI) containing compounds in the fumes. Inductive Coupled Plasma – Mass Spectrometry (ICP-MS), Ion Chromatography (IC) and Fourier Transform Infra-Red Spectroscopy (FTIR) were used primarily to obtain these findings, coupled with statistical techniques such as Pearson’s Correlation Coefficient.
Effect of Life-space on Quality of life of Older Adult in Chinese Communities: the Chain Mediating Role of Social Support and Subjective Well-being
Authors: zhang LJ , Chen WJ , Hong X , Li GR , Wu HB 
Published: May 2023

Abstract
OBJECTIVE To explore the factors influencing the quality of life of older adult by assessing their current life-space, and to examine the mechanisms mediating the effects of social support and Subjective Well-being on the quality of life in older adults’ life-space.METHODS This study surveyed 311 older adults with the Chinese version of the Life Space Assessment, Generic Quality of Life Inventory-74, Memorial University of Newfoundland Scale of Happiness, and Social Support Scale.RESULTS The results of Pearson correlation analysis showed a two-way correlation between life-space, quality of life, social support, and Subjective Well-being in older adults (r = 1.141, 0.164, 0.294, 0.304,0.447, 0.597, P < 0.001), life-space significantly positively predicted the quality of life of older adults (β = 0.294, t = 5.399, P < 0.001); the analysis of mediating effects showed that social support and Subjective Well-being mediated the chain effect between life-space and quality of life in older adults.CONCLUSION In addition to having a direct impact on older individuals' quality of life, their life-space can also have an indirect impact through social support and Subjective Well-being.
A High-Efficiency Primary-Side Regulation Control Strategy for Half-Bridge LLC Resonant Converter with Wide Input Voltage Range
Authors: Dai Mian , Sun Daying , Wei Xinyu , Cong Lihui , Wang Chong 
Published: May 2023

Abstract
The requirement of the half-bridge LLC resonant converter with a wide input voltage range is becoming higher in photovoltaic applications because of its simple structure and low switching loss. Conventional frequency modulation (FM) requires a wide switching frequency range and a high-quality factor circuit design, leading to reduced efficiency and large component volumes at light loads. To solve the problems, a high-efficiency control strategy using adaptive pulse-width and frequency modulation (APWFM) is proposed. APWFM adjusts the gain by changing the switching frequency and duty cycle simultaneously. When the output power is below the reference value, the switching frequency decreases linearly as the output power decreases, and the duty cycle is simultaneously modulated to achieve constant output voltage, so the switching frequency variation range is smaller than FM. This results in improved light or medium load efficiency in a limited frequency range while keeping a small volume of magnetic components. Also, the proposed control strategy is realized with primary-side regulation (PSR) to eliminate the optocoupler and simplify the control circuit. Experimental results demonstrate a significant improvement in efficiency at medium and light loads compared to FM, and the average efficiency is improved by 5% based on low cost and simple operation.
First report on comparative assessment of real-time polymerase chain reaction to standard PCR and microscopy for detection of Babesia bigemina in cattle and buffaloes of North India.
Authors: Kaur Paramjit , Juyal Paryag Dutt , Sharma Amrita , Chachra Deepti , Mukhopadhyay Chander Sekhar , Singla Lachhman Das 
Published: May 2023

Abstract

Purpose: Detection efficacy of real-time PCR targeting 18s SSU ribosomal RNA (18S rRNA) gene of Babesia bigemina in comparison to standard PCR and classical microscopy was evaluated from the samples suspected to be positive for bovine babesiosis.
Materials & methods: Blood samples (n =95) suspected to be positive B. bigemina based on clinical signs and history of tick infestation were analyzed comparatively by three techniques. Real-time PCR was standardized targeting the 18s rRNA gene with the 10 fold serial dilutions in duplication of the given positive control (2x106 copy number) ranging from 106- 100 copy number/µL and mean Ct value of each dilution was taken to extrapolate the curve.
Results: Blood samples (n=95) analysis revealed 5.26, 22.10 and 51.58% to be positive by microscopy, standard PCR and real-time PCR, respectively. Samples positive by microscopy, PCR and real-time PCR were cut down in range of >106-104, 104-103, and 103-<10 copy number/ µL, respectively of 18s rRNA gene. The concordance of real-time PCR with conventional PCR and microscopy was moderate (Kappa= 0.523) and mild (Kappa= 0.09), respectively. Real-time PCR assay indicated cows to be at four times more at risk than buffaloes (Odds ratio:3.85, CI:1.4255 - 10.4370) for getting B. bigemina infection and also indicated as most important risk factor associated with the prevalence of babesiosis.
Conclusion: Results of this first report on comparative analysis indicated real-time PCR to be more perceptive than conventional PCR and microscopy. This needs further investigations on large random sample size to evaluate the factual depiction.
lncRNA: a promising target for cataracts
Authors: dong xiaoming , liu yuxuan , ji liyang , wang jing , zhang jisong 
Published: May 2023

Abstract
Long noncoding RNAs (lncRNAs) are nonprotein-coding transcripts containing >200 nucleotides. lncRNA was first considered the “noise” of genome transcription and had no biological function. With the deepening of research, lncRNA has been found to regulate gene expression at epigenetic, transcriptional, and translation levels, thus affecting cell proliferation, apoptosis, viability, immune response, and oxidative stress. More and more studies have shown that the expression and function of lncRNA are closely related to ophthalmic diseases, such as cataracts, glaucoma, and diabetic retinopathy. This review summarized the classification and mechanism of known lncRNAs and the role of lncRNAs in eye diseases, especially in cataracts, and further clarified their mechanism.
Comparison of metabolic risk factors, lipid indices, healthy eating index and physical activity among premenopausal, menopausal, and Postmenopausal women
Authors: Moradi Leila , Hashemi Sayed Jalal , Zaman Ferdos , Alipour Meysam , Farhangiyan Zahra , Sharifzadeh Maryam 
Published: May 2023

Abstract
Introduction: The prevalence of cardiovascular diseases increases with age in women, especially at menopause. Metabolic syndrome is one of the important factors that cause heart disease. Therefore, the importance screening of people at risk of this syndrome, especially with cheap tests, led to the introduction of new cardio metabolic indicators such as AIP, TyG and WWI. Also, due to the importance of healthy eating and physical activity in preventing metabolic syndrome, more emphasis should be placed on their use.Methods In this cross-sectional study, the number of 4732 women participating in the Hoveyzeh cohort study, after considering the entry and exit criteria, were placed into three groups: pre-menopausal (n = 736), menopausal (n = 396) and post-menopausal (n = 917).Results The prevalence of metabolic syndrome was 43.3%, 55.6%, and 62.8% in pre menopause, menopause, and post menopause, respectively. The prevalence of hypertension (50.2%), dyslipidemia (61.2%), diabetes (37.7%) and abdominal obesity according to the Iranian definition (75.9%) was higher after menopause than before menopause. Cardiovascular disease had the highest prevalence after menopause (23%). The WWI index had the highest OR among the indices with a value of 2.94 and 1.93 in menopause and after menopause, respectively. (P < 0.001). In the HEI-2015 survey, the consumption of total fruit, vegetables, seafood and protein in premenopausal women was more than after menopause, and the consumption of foods containing sugar in menopause was more than in premenopausal women. The amount of physical activity had the highest and lowest values ​​in pre menopause and post-menopause, respectively. (P < 0.001)Conclusion Menopause leads to an increase in the prevalence of metabolic syndrome. The indices of AIP, TyG index, WWI and physical activity increase in women after menopause compared to before menopause. TyG-INDEX ،WWI ،HEI-2015 healthy eating index in this study did not show significant differences between groups after multi-regression analysis.
Textural analysis of MR images as an additional evaluation tool of Parotid Glands in Sjögren's Syndrome in children and adolescents - initial findings
Authors: Grzywińska Małgorzata , Tuzimek Magdalena , Pomorska Anna , Irga-Jaworska Ninela , Świętoń Dominik 
Published: May 2023

Abstract
Magnetic Resonance Imaging (MRI) is often a reference imaging method in soft tissue pathologies, particularly of the head and neck region. A significant increase in MRI popularity is observed, especially concerning salivary gland pathologies like neoplasm or chronic inflammatory processes of salivary glands like in Sjogren syndrome. The development of MRI techniques like MR sialography set a new non-invasive method alternative to classical sialography, especially in advanced cases. Another rising MRI technique - texture analysis - appears to be a promising tool in assessing structural changes in parenchymal organs and can be an additional parameter for assessing the inflammatory process in salivary glands. This research aimed to explore the correlation between apparent diffusion coefficient (ADC), based on T2 weighted images MRI sialography of parotid in children with and without Sjögren's Syndrome (SS). Both groups (20 healthy and 36 patients with SS) underwent 3.0 Tesla MRI, including diffusion-weighted imaging (DWI) (b = 0, 500 and 1000 s/mm2) and T2-weighted imaging. First-order statistics (FSO), second-order, and higher-order statistical texture analysis of the bilateral parotid glands were performed through texture analysis. A multimodal analysis, including texture analysis (TA) clinical classification; MRI evaluation for this disease entity to identify factors of disease activity. The examination group with SS was divided by the Tonami scale. The parotid ADC values from whole volume ROI were significantly lower in group 1 than in the higher activity group. The lowest kurtosis values in the highest grade of the Tonami Scale were found. The FSO parameters showed a positive correlation in TA from T2 weighted images- a moderate correlation with individual parameters. Our findings make TA a promising tool for assessing the grade of parotid gland inflammation. However, the effect of binning and ROI (region of interest) size is yet to be determined and needs further studies.
Nonlinearity Association between Hyperuricemia and All-cause Mortality of Patients with Chronic Kidney Disease
Authors: Liu Ya-Fei , Han Liang , Geng Yin-Hong , Wang Huan-Huan , Yan Jia-Hui , Tu Sheng-Hao 
Published: May 2023

Abstract

Background: Controversy surrounds the role of serum uric acid and whether treatment intervention is favorable in retarding the progress of chronic kidney disease (CKD). The association of serum uric acid levels and CKD patients’ mortality risk needs to furtherly be determined by large sample cohort studies. Methods: The National Health and Nutrition Examination Survey (NHANES) participants with CKD patients from 1998 to 2017 were enrolled to the study. Multivariable Cox regression models were used to reveal the association of serum uric acid concentrations and CKD mortality risks. Results: 9891 CKD patients were enrolled to the study and 3698 individuals deceased during the follow-up. Increasing serum uric acid levels are independently relevant to higher mortality risks of CKD patients (HR per SD increase). A restricted cubic spline curve showed a nonlinear association between serum uric acid and CKD mortality risks (pfor nonlinearity = 0.046). CKD patients with higher levels of serum uric acid (≥ 5.900 mg/dL) show a significant increment of mortality risks (HR = 1.102, 95% CI: 1.043-1.165). Sensitivity analysis demonstrated that the results were stable and robust. Conclusions: High serum uric acid levels (≥ 5.900 mg/dL) may be associated with increasing mortality risks in CKD patients. CKD populations with high serum uric acid may benefit from lowering the serum uric acid.
Identification and validation of autophagy-related genes in primary open-angle glaucoma
Authors: Xu Wanjing , Sun Yuhao , Zhao Shuang , Zhao Jun , Zhang Juanmei 
Published: May 2023

Abstract
Background As the most common type of glaucoma, the etiology of primary open-angle glaucoma (POAG) has not been unified. Autophagy may affect the occurrence and development of POAG, while the specific mechanism and target need to be further explored.Methods The GSE27276 dataset from the Gene Expression Omnibus (GEO) database and the autophagy gene set from the GeneCards database were selected to screen differentially expressed autophagy-related genes (DEARGs) of POAG. Hub DEARGs were selected by constructing protein-protein interaction (PPI) networks and utilizing GSE138125 dataset. Subsequently, immune cell infiltration analysis, genome-wide association study (GWAS) analysis, gene set enrichment analysis (GSEA) and other analyses were performed on the hub genes. Eventually, animal experiments were performed to verify the mRNA levels of the hub genes by quantitative real time polymerase chain reaction (qRT-PCR).Results A total of 67 DEARGs and 2 hub DEARGs, HSPA8 and RPL15, were selected. The hub genes were closely related to the level of immune cell infiltration. GWAS analysis confirmed that the causative regions of the 2 hub genes in glaucoma were on chromosome 11 and chromosome 3, respectively. GSEA illustrated that pathways enriched for highly expressed HSPA8 and RPL15 contained immunity, autophagy, gene expression and energy metabolism-related pathways. qRT-PCR confirmed that the expression of HSPA8 and RPL15 in the rat POAG model was consistent with the results of bioinformatics analysis.Conclusions This study indicated that HSPA8 and RPL15 may affect the progression of POAG by regulating autophagy and provided new ideas for the pathogenesis and treatment of POAG.
DAPT improves behavioral abnormalities and rescues the hippocampal Oligodendrocyte precursor cell differentiation in cuprizone-induceddemyelination mouse model
Authors: Chen Fei-fei , Che Ying , Mi Xiao-juan , Wu Xiao-min , Zhang Chun , Shao Yu , Wei Hui-ling , Feng Zi-qi , Li Jun-jie , Liu Juan 
Published: May 2023

Abstract
Introduction: Oligodendrocyte precursor cells (OPCs) differentiation dysfunction is closely related to demyelinating diseases and cognitive dysfunction. P75 neurotrophin receptor (P75NTR) is a prototypical co-receptor that induces Schwann cell death via γ-secretase-dependent regulated intramembrane proteolysis. This study hypothesizes that P75NTR may also assists in inhibiting OPCs differentiation.Methods Male C57BL/6 mice were fed 0.2% cuprizone (CPZ) continuously for 6 weeks to establish the acute demyelinating model (CPZ mice). Morris Water Maze and Elevated Plus Maze tests were used to assess the behavioral changes of these mice. Immunohistochemistry and Western blot were used to detect the OPCs and oligodendrocytes (OLs) protein markers. Furthermore, γ-secretase inhibitor DAPT (GSI-IX) was injected into the hippocampus at the fifth week of establishing the demyelinating model to investigate the effects of DAPT on OPCs differentiation and the mice’s behavioral changes.Results CPZ mice performed abnormal behavioral changes, and the protein expression of the OLs marker 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase) decreased. However, the OPCs marker neural/glial antigen 2 (NG2) protein expression increased. After DAPT treatment, the abnormal behavior improved, CNPase increased, and NG2 decreased.Conclusions P75 cleavage plays an inhibitory role during the OPCs differentiation resulting in inefficient OPCs differentiation and recurrent demyelinating diseases.