bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene therapy using optimized LentiHBBT87Qvector in two patients with transfusion dependent β-thalassemia
Authors: Han Nan , Li Yue , Ouyang Wenjie , Dong Guoyi , Guo Honglian , Chen Yue , Huang Yan , Zeng Xinru , Zou Huilin , He Jiajun , Yao Wenwen , Liu Chao , Liu Sixi 
Published: March 2023

AbstractBackgroundGene therapy is gradually becoming recognized as a possibly curative therapeutic strategy for transfusion-dependent β-thalassemia (TDT). Gene therapy addresses the problem of donor scarcity through the application of autologous hematopoietic stem cells (HSCs), which also can reduce the risks that accompany allogeneic HSC transplantation. When using gene addition strategy, lentiviral vector is critical for the efficacy and safety of β-thalassemia gene therapy. In our preclinical studies, LentiHBBT87Qvector with optimized backbone was developed to efficiently restore β–globin expression in HSCs-derived erythroblasts of TDT patients with minimal risk of tumorigenesis. Here, we presented the clinical trial results of gene therapy using LentiHBBT87Qvector in two TDT patients.MethodIn an ongoing phase 1/2 trial (NCT05745532), auto-HSCs were mobilized from two TDT patients, and then transduced with LentiHBBT87Qvector. The gene-modified auto-HSCs is called HGI-001 injection. After four-day consecutive myeloablative conditioning, these two patients were administrated with HGI-001 injection via intravenous infusion. Medical examinations were performed in the transplantation unit to monitor patients’ status till the patients were clinically stable. Then, 24-month following-up visits are conducted to assess the safety and efficacy of HGI-001 injection. The safety endpoints of this clinical study include the incident and severity of adverse events (AEs); transplant-related mortality or disability events within 100 days post drug product infusion; vector-related replication competent lentivirus (RCL) and clonal variations containing specific viral integration sites; overall survival during this clinical trial. The major efficacy endpoint is the percentage of subjects with average vector copy number (VCN) in peripheral blood mononuclear cells (PBMCs) >0.1, and average expression of exogenous HbAT87Q>2.0g/dL at the 24thmonth after reinfusion of HGI-001 injectionResultsThe rapid neutrophil and platelet engraftment successfully happened after reinfusion of HGI-001 injection. The two patients with non-β0/β0genotype have been transfusion-independent for 24 months and 21 months post-treatment. At the last visit, the levels of HbAT87Qare 7.3 and 6.9g/dL, and the levels of total hemoglobin are 9.8 and 10.1 g/dL. After the two subjects stopped transfusions, the iron overload has been alleviated without iron chelation treatment. Most AEs are myeloablative conditioning related, and can be controlled through clinically standard therapeutic managements. No clone dominance related to vector integration nor RCL has been observed.ConclusionGene therapy with optimized LentiHBBT87Qvector (HGI-001 injection) assist two TDT patients become transfusion-independent without serious adverse events related to the product.
“Informative Use of Cycle-Threshold Values to Account for Sampling Variability in Pathogen Detection”
Authors: Nikiforuk Aidan M. , Jassem Agatha N. 
Published: March 2023

AbstractNucleic acid amplification tests, like real-time polymerase chain reaction, are widely used for pathogen detection; however, their interpretation rarely accounts for sampling variability. Instead, cycle threshold values are often categorized reducing precision. We describe how pathogen cycle threshold values can be normalized to endogenous host gene expression to correct for sampling variability and compare the validity of this approach to standardization with a standard curve. Normalization serves as a valid alternative to standardization, does not require making a standard curve, increases precision, accounts for sampling variability, and can be easily applied to large clinical or surveillance datasets for informative interpretation.
The Prevalence and Management of Atrial Fibrillation in New Zealand Māori Detected through an Abdominal Aortic Aneurysm Screening Programme
Authors: Sandiford P. , Poppe K. , Grey C , Doughty R. , Chambers E. , Kim K.J. , Hill A. , Bartholomew K. 
Published: March 2023

ABSTRACTBackgroundAtrial fibrillation (AF) screening was incorporated into an abdominal aortic aneurysm screening (AAA) programme for New Zealand (NZ) Māori.MethodsAF screening was performed as an adjunct to AAA screening of Māori men aged 60-74 and women aged 65-74 registered with primary health care practices in Auckland, NZ. Pre-existing AF was determined through coded diagnoses or medications in the participant’s primary care record. Subsequent audit of the record assessed accuracy of pre-screening coding, medication use and clinical follow-up.ResultsAmong 1955 people screened, the prevalence of AF was 144 (7.4%), of which 46 (2.4% of the cohort) were patients without AF coded in the medical record. More than half of these were revealed to be known AF but that was not coded. Thus, the true prevalence of newly detected AF was 1.1% (n=21). An additional 48 (2.5%) of the cohort had been coded as AF but were not in AF at the time of screening. Among the 19 at-risk screen-detected people with AF, 10 started appropriate anticoagulation within 6 months. Of the 9 who did not commence anticoagulation, five had a subsequent adverse clinical outcome in the follow-up period, including one with ischaemic stroke; two had contraindications to anticoagulants. Among those with previously diagnosed AF, the proportion receiving anticoagulation rose from 57% pre-screening to 83% at 6 months post-screening (p<0.0001); among new AF the proportion rose from 0% to 53% (p<0.01).ConclusionsThere is a high prevalence of AF in NZ Māori. AF screening is a feasible low-cost adjunct to AAA screening with potential to detect previously undiagnosed AF and reduce ethnic inequities in stroke. It may also prompt better coding and management of previously diagnosed AF. However, effective fail-safe measures are needed to ensure that high-risk newly diagnosed AF is managed according to best practice guidelines.
Urgent Transcatheter Edge-to-Edge Repair for Severe Mitral Regurgitation with Flail Leaflet in Critically Ill Patients
Authors: Perel Nimrod , Amsalem Itshak , Gilad Or , Hitter Rafael , Maller Tomer , Asher Elad , Harari Emanuel , Marmor David , Carasso Shemy , Dvir Danny , Glikson Michael , Shuvy Mony 
Published: March 2023

AbstractIntroductionDegenerative mitral valve disease (DMR) is a common valvular disorder, and flail leaflet due to ruptured chordae represents an extreme variation of this pathology. Ruptured chordae can present as acute heart failure which requires urgent intervention. Mitral valve surgery is the preferred mode of intervention; however, some patients represent as a significant surgical risk and are often deemed to be inoperable. We aim to characterize patients with ruptured chordae undergoing urgent transcatheter edge-to-edge repair (TEER), and to analyze their clinical and echocardiographic outcomes.MethodsAt a referral center for several hospitals across Israel, we screened all patients who underwent TEER. We included patients with DMR with flail leaflet due to ruptured chordae, and categorized them into elective and critically ill groups. We evaluated the echocardiographic, hemodynamic and clinical outcomes of these patients.ResultsThe cohort included 49 patients with DMR due to ruptured chordae and flail leaflet, who underwent TEER. Seventeen patients (35%) underwent urgent intervention and 32 patients (65%) underwent an elective procedure. In the urgent group, the average age of the patient was 80.3, with 41.8% being female. Fourteen (82%) received noninvasive ventilation, while three patients (18%) required invasive mechanical ventilation. One patient died due to tamponade, while the other 16 patients all had successful reduction of ≥2 in the MR grade. Left atrial V wave decreased from 41.6 mmHg to 17.9 mmHg (p<0.001), and pulmonic vein flow pattern changed from reversal (68.8%) to a systolic dominant flow in all patients (p=0.001). After the procedure, 78.5% of patients were in New York Heart Association (NYHA) class I or II (p<0.001). There was no significant difference in the overall mortality between the urgent and elective groups, with similar survival rates of 6 months for each group.ConclusionTEER can be safe and feasible with favorable hemodynamic, echocardiographic and clinical outcomes in patients undergoing urgent intervention for flail mitral valve disease.Clinical PerspectivesMitral transcatheter edge-to-edge repair (TEER) is a proven therapy for patients with chronic mitral regurgitation, however data on patients with acute mitral regurgitation limited. This article focuses on acute TEER intervention and provides further information on the safety, feasibility and effectiveness of TEER in critically ill patients due to flail leaflet.The clinical finding supports the role of acute TEER intervention in acutely ill patients with acute mitral regurgitation due to ruptured chordae and flail leaflet.
Attribute-based choice model and willingness to pay for industrial services
Authors: Rakesh V.T. , Menon Preetha , Raman Ramakrishnan 
Published: March 2023

PurposePricing is widely acknowledged as a market entry challenge for servitising companies. The purpose of this research is to ascertain the attributes that contribute to willingness to pay (WTP) for industrial services and suggest incorporating those attributes to a pricing model.Design/methodology/approachThree attributes (Quality of Service, Nearness of Service Provider and Brand Equity of Service Provider) were analyzed at three respective levels to ascertain their importance on WTP. Conventional conjoint analysis (CCA), using an orthogonal design, was the method used. The 346 respondents were decision-makers and top management professionals from various industries.FindingsBrand Equity emerged as the most significant attribute contributing to WTP, having more than 45% importance – followed by the Quality and Nearness.Research limitations/implicationsThe scope of the study is limited to the industries and its Allies. However, the relative importance of the attributes may vary depending on the type of service.Practical implicationsThe importance of attributes and their WTP preference helps future researchers create a pricing model involving these attributes. This helps service providers price their services rationally, thus succeeding in servitization.Social implicationsProduct life is extended because the manufacturers themselves are servicing it and also help recycle the product with their expertise. Servitization is also helpful for the Indian economy, as it is turning into a manufacturing economy.Originality/valueThis research investigates three attributes that contribute to WTP, in accordance with their level of contribution. It also provides a direction to establish an adequate pricing model for industrial services.
Fractional equivalent circuit model and parameter identification of reactance components in high-frequency operation
Authors: Li Yixuan , Chen Yanfeng , Zhang Bo , Qiu Dongyuan , Xie Fan , Cheng Chao 
Published: March 2023

Purpose
The purpose of this paper is to find a simpler model for the reactance components in the high-frequency range on the premise of ensuring the accuracy.

Design/methodology/approach
In this paper, based on the fractional calculus theory and the traditional integer-order model, a reactance model suitable for high frequency is constructed, and the mutation cross differential evolution algorithm is used to identify the parameters in the model.

Findings
By comparing the integer-order model, high-frequency fractional-order model and the actual impedance characteristic curve of inductance and capacitance, it is verified that the proposed model can more accurately reflect the high-frequency characteristics of inductance and capacitance. The simulation and experimental results show that the oscillator constructed based on the proposed model can analyze the frequency and output waveform of the oscillator more accurately.

Originality/value
The model proposed in this paper has a simple structure and contains only two parameters to be identified. At the same time, the model has high precision. The fitting errors of impedance curve and phase-frequency characteristic curve are less than 5%. Therefore, the proposed model is helpful to improve the simplicity and accuracy of circuit system analysis and design.
A tree dries from the top: how manager’s knowledge hiding is morally disengaging employees to hide knowledge
Authors: Khan Muhammad Mumtaz , Mubarik Muhammad Shujaat , Ahmed Syed Saad , Islam Tahir 
Published: March 2023

Purpose
The purpose of this study was to explicate how leaders’ knowledge hiding results in employees’ knowledge hiding. In addition, the study was intended to explore under what conditions leaders’ knowledge hiding affects employees’ moral disengagement more deleteriously.

Design/methodology/approach
Data were collected from 321 employees at three different times which were two months apart from each other. Structural equation modeling was used for data analysis.

Findings
The study found leaders’ knowledge hiding to be related to employee moral disengagement. In addition, the study found moral disengagement to affect employees’ knowledge-hiding behavior. Moral disengagement was found to mediate the relationship between leaders’ knowledge hiding and employees’ knowledge hiding. Finally, the study found that employees with high moral identity show more perseverance to preserve their moral engagement when led by knowledge-hiding leaders.

Originality/value
To the best of the authors’ knowledge, the study was first to establish a relationship between a leader’s knowledge hiding and employees’ moral disengagement. The study also established the mediating role of moral disengagement to work as a mediating mechanism linking leaders’ knowledge hiding to employees’ knowledge hiding. Finally, the study found that moral identity moderates the relationship between leaders’ knowledge hiding and employees’ moral disengagement.
Contributing to sustainable development goals (SDGs) in environmental sustainability through public-private investment in energy: empirical evidence from EAGLE economies
Authors: Tabash Mosab I. , Farooq Umar , Anagreh Suhaib , Al-Faryan Mamdouh Abdulaziz Saleh 
Published: March 2023

Purpose
This study aims to explore the empirical relationship between public–private investment (PPI) in energy and environmental quality.

Design/methodology/approach
The authors hypothesize that PPI can reduce pollution emissions and test this hypothesis by sampling the 20-year data of emerging and growth-leading economies (EAGLE) and adopting two estimation techniques named panel estimated generalized least square and fully modified ordinary least square models.

Findings
The empirical analysis vows that PPI has an inverse relationship with CO2 emissions, corroborating the sustainable development driving role of PPI. In addition, the empirical outcomes suggest a negative/positive role of energy imports and economic growth. Meanwhile, foreign direct investment is negatively linked with CO2 emissions, corroborating the pollution halo hypothesis in the case of EAGLE. However, financial development shows a positive relationship with CO2 emissions.

Practical implications
This study offers an important policy outlay regarding the pollution mitigation role of PPI in EAGLE. The environmental sustainability in underlying economies can be achieved by enhancing the magnitude of public–private cooperation in energy investment. The empirical analysis supplements cutting-edge empirical evidence regarding PPI as a driver of important sustainable development goal (SDG), i.e. environmental sustainability.

Originality/value
To the best of the authors’ knowledge, this study is the first study that examines how one can achieve an important SDG regarding environmental sustainability through PPI in energy.
A robust and simple collision avoidance approach for pick and place robot applications
Authors: Deniz Cengiz 
Published: March 2023

Purpose
The aim of this study is to create a robust and simple collision avoidance approach based on quaternion algebra for vision-based pick and place applications in manufacturing industries, specifically for use with industrial robots and collaborative robots (cobots).

Design/methodology/approach
In this study, an approach based on quaternion algebra is developed to prevent any collision or breakdown during the movements of industrial robots or cobots in vision system included pick and place applications. The algorithm, integrated into the control system, checks for collisions before the robot moves its end effector to the target position during the process flow. In addition, a hand–eye calibration method is presented to easily calibrate the camera and define the geometric relationships between the camera and the robot coordinate systems.

Findings
This approach, specifically designed for vision-based robot/cobot applications, can be used by developers and robot integrator companies to significantly reduce application costs and the project timeline of the pick and place robotics system installation. Furthermore, the approach ensures a safe, robust and highly efficient application for robotics vision applications across all industries, making it an ideal solution for various industries.

Originality/value
The algorithm for this approach, which can be operated in a robot controller or a programmable logic controller, has been tested as real-time in vision-based robotics applications. It can be applied to both existing and new vision-based pick and place projects with industrial robots or collaborative robots with minimal effort, making it a cost-effective and efficient solution for various industries.
A bibliometric analysis of artificial intelligence chatbots in educational contexts
Authors: Lin Yupeng , Yu Zhonggen 
Published: March 2023

Purpose
The application of artificial intelligence chatbots is an emerging trend in educational technology studies for its multi-faceted advantages. However, the existing studies rarely take a perspective of educational technology application to evaluate the application of chatbots to educational contexts. This study aims to bridge the research gap by taking an educational perspective to review the existing literature on artificial intelligence chatbots.

Design/methodology/approach
This study combines bibliometric analysis and citation network analysis: a bibliometric analysis through visualization of keyword, authors, organizations and countries and a citation network analysis based on literature clustering.

Findings
Educational applications of chatbots are still rising in post-COVID-19 learning environments. Popular research issues on this topic include technological advancements, students’ perception of chatbots and effectiveness of chatbots in different educational contexts. Originating from similar technological and theoretical foundations, chatbots are primarily applied to language education, educational services (such as information counseling and automated grading), health-care education and medical training. Diversifying application contexts demonstrate specific purposes for using chatbots in education but are confronted with some common challenges. Multi-faceted factors can influence the effectiveness and acceptance of chatbots in education. This study provides an extended framework to facilitate extending artificial intelligence chatbot applications in education.

Research limitations/implications
The authors have to acknowledge that this study is subjected to some limitations. First, the literature search was based on the core collection on Web of Science, which did not include some existing studies. Second, this bibliometric analysis only included studies published in English. Third, due to the limitation in technological expertise, the authors could not comprehensively interpret the implications of some studies reporting technological advancements. However, this study intended to establish its research significance by summarizing and evaluating the effectiveness of artificial intelligence chatbots from an educational perspective.

Originality/value
This study identifies the publication trends of artificial intelligence chatbots in educational contexts. It bridges the research gap caused by previous neglection of treating educational contexts as an interconnected whole which can demonstrate its characteristics. It identifies the major application contexts of artificial intelligence chatbots in education and encouraged further extending of applications. It also proposes an extended framework to consider that covers three critical components of technological integration in education when future researchers and instructors apply artificial intelligence chatbots to new educational contexts.
COVID-19, livestock supply chains and livelihoods in India: a review of impacts and implications
Authors: Verma Gautami , Singla Naresh , Singh Sukhpal 
Published: March 2023

Purpose The global outbreak of COVID-19 and its subsequent varied impacts on different economic activities necessitate to examine its disruptions and impacts on livestock sector in India due to its recently surging potential as an unrivaled alternative to boost farmer’s income.Design/methodology/approachThe studies for review were identified through search in different databases using relevant keywords. Only full text papers written in English language were reviewed. The review was organized and streamlined using Covidence software.FindingsAnalysis of the literature reveals adverse effects of COVID-19 on functioning of input and output stages of livestock supply chains. This has resulted in upstream and downstream economic losses that affect livelihoods of the producers.Research limitations/implicationsScale of unprecedented crisis due to COVID-19 pandemic requires creative policy decisions to make livestock production systems robust, resilient and sustainable. Organized production systems are required to integrate with livestock-tech startups to modernize their supply chains, whereas local supply chains are required to reorient with government’s intervention in terms of developing on-farm production and postproduction processing facilities.Originality/valueAlthough there exist some evidence on COVID-19-related impacts on livestock sector of India, but an integrated review of evidence on COVID-19 related disruptions at all the stages (from input supply to marketing) of livestock supply chains was missing.