bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole lung radiomic features are associated with overall survival in patients with locally advanced non-small cell lung cancer treated with definitive radiotherapy
Authors: Yan Meng , Zhang Zhen , Tian Jia , Yu Jiaqi , Dekker Andre , Ruysscher Dirk de , Wee Leonard , Zhao Lujun 
Published: January 2025

Abstract

Background
Several studies have suggested that lung tissue heterogeneity is associated with overall survival (OS) in lung cancer. However, the quantitative relationship between the two remains unknown. The purpose of this study is to investigate the prognostic value of whole lung-based and tumor-based radiomics for OS in LA-NSCLC treated with definitive radiotherapy.

Methods
A total of 661 patients with LA-NSCLC treated with definitive radiotherapy in combination with chemotherapy were enrolled in this study, with 292 patients in the training set, 57 patients from the same hospital from January to December 2017 as an independent test set (test-set-1), 83 patients from a multi-institutional prospective clinical trial data set (RTOG0617) as test-set-2, and 229 patients from a Dutch radiotherapy center as test-set-3. Tumor-based radiomic features and whole lung-based radiomic features were extracted from primary tumor and whole lungs (excluding the primary tumor) delineations in planning CT images. Feature selection of radiomic features was done by the least absolute shrinkage (LASSO) method embedded with a Cox proportional hazards (CPH) model with 5-fold cross-internal validation, with 1000 bootstrap samples. Radiomics prognostic scores (RS) were calculated by CPH regression based on selected features. Three models based on a tumor RS, and a lung RS separately and their combinations were constructed. The Harrell concordance index (C-index) and calibration curves were used to evaluate the discrimination and calibration performance. Patients were stratified into high and low risk groups based on median RS, and a log-rank test was performed.

Results
The discrimination ability of lung- and tumor-based radiomics model was similar in terms of C-index, 0.69 vs. 0.68 in training set, 0.68 vs. 0.66 in test-set-1, 0.61 vs. 0.62 in test-set-2, 0.65 vs. 0.64 in test-set-3. The combination of tumor- and lung-based radiomics model performed best, with C-index of 0.71 in training set, 0.70 in test-set-1, 0.69 in test-set-2, and 0.68 in test-set-3. The calibration curve showed good agreement between predicted values and actual values. Patients were well stratified in training set, test-set-1 and test-set-3. In test-set-2, it was only whole lung-based RS that could stratify patients well and tumor-based RS performed bad.

Conclusion
Lung- and tumor-based radiomic features have the power to predict OS in LA-NSCLC. The combination of tumor- and lung-based radiomic features can achieve optimal performance.
Hypoxia impairs decitabine-induced expression of HLA-DR in acute myeloid leukaemia cell lines
Authors: Humphries Sam , Burnard Sean M. , Eggins Courtney D. , Keely Simon , Bond Danielle R. , Lee Heather J. 
Published: January 2025

Abstract

Background
Hypomethylating agents (HMA), such as azacytidine (AZA) and decitabine (DAC), are epigenetic therapies used to treat some patients with acute myeloid leukaemia (AML) and myelodysplastic syndrome. HMAs act in a replication-dependent manner to remove DNA methylation from the genome. However, AML cells targeted by HMA therapy are often quiescent within the bone marrow, where oxygen levels are low. In this study, we investigate the effects of hypoxia on HMA responses in AML cells.

Results
AML cell lines (MOLM-13, MV-4-11, HL-60) were treated with DAC (100 nM) or AZA (500–2000 nM) in normoxic (21% O2) and hypoxic (1% O2) conditions. Hypoxia significantly reduced AML cell growth across all cell lines, with no additional effects observed upon HMA treatment. Hypoxia had no impact on the extent of DNA hypomethylation induced by DAC treatment, but limited AZA-induced loss of methylation from the genome. Transcriptional responses to HMA treatment were also altered, with HMAs failing to up-regulate antigen presentation pathways in hypoxia. In particular, cell surface expression of the MHC class II receptor, HLA-DR, was increased by DAC treatment in normoxia, but not hypoxia.

Conclusion
Our results suggest that HMA-induced antigen presentation may be impaired by hypoxia. This study highlights the need to consider microenvironmental factors when designing co-treatment strategies to improve HMA therapeutic efficacy.
Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7- and pH-dependent manner
Authors: Liang Qi-Zhang , Ji Chun-Miao , Wang Bin , Chen Wei , Cong Feng , Huang Yu , Huang Yao-Wei 
Published: January 2025

Abstract
Porcine deltacoronavirus (PDCoV), also known as HKU15, is a swine enteropathogenic virus that is believed to have originated in birds. PDCoV belongs to the genus Deltacoronavirus (DCoV), the members of which have mostly been identified in diverse avian species. We recently reported that chicken or porcine aminopeptidase N (APN), the major cellular receptor for PDCoV, can mediate cellular entry via three pseudotyped retroviruses displaying spike proteins from three avian DCoVs (HKU11, HKU13, and HKU17). In the present work, to better understand how avian-origin CoVs may be transmitted to pigs, we investigated the unknown DCoV entry pathway in avian cells. We show that clathrin-mediated endocytosis is involved in the entry of these DCoV pseudoviruses into chicken-origin DF-1 cells. Pseudovirus entry was suppressed by means of pharmacological inhibitors, dominant-negative mutants, and siRNAs targeting various cellular proteins and signalling molecules, suggesting that PDCoV and avian DCoV pseudovirus entry into DF-1 cells depends on clathrin, dynamin-2, cathepsins and a low-pH environment but is independent of caveolae and macropinocytosis. Furthermore, we found that DCoV pseudovirus entry was linked to Rab5- and Rab7-dependent pathways. This is the first report demonstrating that these DCoVs utilize clathrin-mediated endocytosis pathways to enter avian-origin cells, providing new insights into interspecies transmission of DCoVs.
An overview of the transboundary dust events in southeastern Turkmenistan near the border of Iran (Sarakhs-Badkhyz region)
Authors: Mansouri Daneshvar Mohammad Reza , Rasouli Seyed Javad , Dehqanpour Aliaqa Aliasghar 
Published: January 2025

Abstract
This paper revealed the intensifying transboundary dust events in southeastern Turkmenistan near the border of Iran, using long-term spatial and statistical data (1980–2023). This study introduced the definition of a new source of dust storms, which is called the Sarakhs-Badkhyz region, apart from the Karakum and Kyzylkum deserts. The mentioned dust source with a surface area of 100,000 km2 is located in southeastern Turkmenistan and northeastern Iran. The remotely sensed investigation of this study was supported with local and international re-analyzed datasets (e.g., IRIMO, Eumetsat, and NASA). A drastic temperature increase triggers evaporation and also dryness of the sandy deserts in southeastern Turkmenistan. Then, dried sandy grounds and high wind speeds are important factors in creating favorable conditions for dust storm formation. Additionally, the increase in the intensity of general atmospheric circulation and the more energetic activity of the frontal cyclones, associated with the cold air intrusion of the Siberian anticyclone, are important mechanisms in the dust events of the study area. The decrease in precipitation in recent years in addition to declining the water flows of the Tejen and Murghab rivers from their basins is the reason for the dryness of the sandy ground of the Sarakhs-Badkhyz region. As a result, the defined region is a susceptible zone to source sediments of dust storms in the Middle East. Future research should analyze the possible effects of the dust storms, raised from the Sarakhs-Badkhyz region, on the cultivation, construction, immigration, and healthcare situations of the inhabitants and settlements, dominantly in Mashhad, Mary, and Herat urban regions (with a total of 4.35 million inhabitants).
Compact and cGMP-compliant automated synthesis of [18F]FSPG on the Trasis AllinOne™
Authors: Farooq Rizwan , Gendron Thibault , Edwards Richard S. , Witney Timothy H. 
Published: January 2025

Abstract

Background
(S)-4-(3-18F-Fluoropropyl)-ʟ-glutamic acid ([18F]FSPG) is a positron emission tomography radiotracer used to image system xc
−, an antiporter that is upregulated in several cancers. Not only does imaging system xc
− with [18F]FSPG identify tumours, but it can also provide an early readout of response and resistance to therapy. Unfortunately, the clinical production of [18F]FSPG has been hampered by a lack of robust, cGMP-compliant methods. Here, we report the automated synthesis of [18F]FSPG on the Trasis AllinOne™, overcoming previous limitations to provide a user-friendly method ready for clinical adoption.

Results
The optimised method provided [18F]FSPG in 33.5 ± 4.9% radiochemical yield in just 35 min when starting with 18–25 GBq. Importantly, this method could be scaled up to > 100 GBq starting activity with only a modest reduction in radiochemical yield, providing [18F]FSPG with a molar activity of 372 ± 65 GBq/µmol and excellent radiochemical purity (96.8 ± 1.1%). The formulated product was stable when produced with these high starting activities.

Conclusions
We have developed the first automated synthesis of [18F]FSPG on the Trasis AllinOne™. The method produces [18F]FSPG with excellent radiochemical purity and in high amounts suitable for large clinical trials and off-site distribution. The method expands the number of synthesis modules capable of producing [18F]FSPG and has been carefully designed for cGMP compliance to simplify regulatory approval for clinical production. The methods developed for the purification of high-activity [18F]FSPG are transferrable and should aid the development of clinical [18F]FSPG productions on other synthesis modules.
Hybrydowość kulturowa w przestrzeniach wielokulturowych
Authors: Kuzmany Börries 
Published: January 2025

CULTURAL HYBRIDITY IN MULTICULTURAL REGIONS: HISTORIES OF GALICIAN CULTURE
This article investigates the complex interplay of cultures in Habsburg Galicia, a region marked by its diverse linguistic, religious, and ethnic makeup. It focuses on cultural practices and suggests understanding multilingualism, overarching tropes, and intersecting identities as a form of cultural hybridity. As Galician culture was more than agglutinating several national cultures, this article suggests narrating its history as a plurale tantum. Galician cultural histories are manifest in the province’s theatre and music scene, literary production, architecture and fine arts. The paper tackles both the entangled cultural backgrounds and multilingualism of many authors, artists, and cultural entrepreneurs and recurrent topics that appear throughout Galicia’s cultural production like the Carpathian Mountains or the theme of poverty and religiosity. The article further examines the impact of national movements on the cultural landscape of Galicia. The rise of Polish, Ukrainian, and Jewish nationalisms in the 19th and early 20th centuries led to the formation of distinct cultural institutions and the promotion of specific national narratives. However, the paper argues that these nationalistic projects not only resembled each others in their mechanisms of nationalizing culture, but often coexisted with more cosmopolitan and hybrid cultural practices. This paper argues that despite manifold social, linguistic, religious and national borders and hierarchies being omnipresent, these fractures also interconnected the hybrid cultural space of Galicia.
Efficacy of General Magnetic Therapy and Motor-Evoked Potential-Guided Precision Magnetic Therapy in Patients with Overactive Bladder: A Randomized Controlled Trial
Authors: Xu Hongzhe , Zhu Zhangji , Lv Yinan , Gong Jiawei , Duan Yue 
Published: January 2025

Aims/Background Overactive bladder (OAB) is a prevalent chronic condition affecting approximately 12% of adults, with incidence increasing with age. While pharmacological and behavioural therapies are standard treatments, their efficacy is often limited by side effects and poor adherence. This study aimed to compare the therapeutic effects of precision magnetic stimulation guided by motor-evoked potential with general magnetic therapy in patients with OAB. Methods This randomized controlled trial included 45 patients diagnosed with OAB at The Second Affiliated Hospital of Zhejiang Chinese Medicine University between January 2022 and September 2022. Participants were randomly divided into Group 1 (n = 15) which received bladder training (BT) alone, Group 2 (n = 15) received BT combined with general magnetic therapy, and Group 3 (n = 15) received BT combined with precision magnetic therapy. Clinical efficacy was evaluated using the Overactive Bladder Symptom Score (OABSS), urination diaries, initial bladder volume, and maximum bladder volume before and after treatment. Results At the end of the intervention, Groups 2 and 3 demonstrated significant improvements in OABSS scores, urgency of urination, initial bladder volume, and maximum bladder volume compared to Group 1 (p < 0.05). Group 3 exhibited superior urination frequency and initial bladder volume improvements compared to Group 2 (p < 0.05). Conclusion General magnetic therapy combined with BT and precision magnetic therapy combined with BT were more effective than BT alone in treating OAB. While there were no significant differences between the two magnetic stimulation methods in terms of OABSS scores, urgent urination, nocturnal urination, or maximum bladder volume, precision magnetic therapy showed greater efficacy in reducing urination frequency and improving initial bladder volume. These findings suggest that precision magnetic therapy offers an enhanced therapeutic benefit. Clinical Trial Registration Chinese Clinical Trial Registry ( ChiCTR2400087888 ).
Application of the Diagnosis Grouping System to Pediatric Emergency Department Encounters
Authors: Ramgopal Sriram , Fant Colleen , Marin Jennifer R. , Alpern Elizabeth R. 
Published: January 2025

OBJECTIVE
Diagnosis grouping systems are essential for health services and epidemiological and health outcomes research. The Diagnosis Grouping System (DGS) was developed to classify International Classification of Diseases, 10th Revision diagnosis codes for pediatric emergency department (ED) encounters. We applied and compared the DGS to large samples of US ED encounters for children using 3 large health system databases.

METHODS
We conducted a retrospective cross-sectional study of children (younger than age 18 years) between 2016 and 2021 using 3 ED datasets: the National Hospital Ambulatory Medical Care Survey (NHAMCS; a complex survey of US ED encounters), COMPdata (a dataset of encounters for Illinois), and the Pediatric Health Information Systems (PHIS; an administrative database of US children’s hospitals). We categorized principal diagnoses using the DGS and compared their distribution.

RESULTS
We included 181 607 978 survey-weighted encounters from NHAMCS, 5 352 016 encounters from COMPdata, and 20 859 019 encounters from PHIS. A higher proportion of encounters in the PHIS sample were admitted or transferred (12.7%) compared with those in the NHAMCS (6.1%) or COMPdata (4.2%) samples. Trauma was the most common major group identified among all datasets (22.0% in NHAMCS, 22.2% in COMPdata, and 17.0% in PHIS). Some conditions, such as sickle cell anemia, diabetes mellitus, appendicitis, seizures, and asthma, presented more frequently among children presenting to the PHIS hospitals.

CONCLUSIONS
We identified comparable diagnostic distributions across different datasets, with differences that are likely attributable to specific characteristics of the data source. Our findings provide a baseline for pediatric diagnoses to facilitate future research on pediatric ED utilization.
Maternal gut microbiome and its impact on developing idiopathic late fetal growth retardation
Authors: Zhelezova M. E. , Bektur B. К. , Maltseva L. I. , Sharipova R. I. , Shakirzyanova D. B. 
Published: January 2025

Aim: to analyze a rectal microbiome composition in pregnant women and assess its potential impact on developing of idiopathic late fetal growth retardation (FGR) syndrome.Materials and Methods. We analyzed a course of pregnancy and labour in 80 pregnant women: main group consisted of 40 pregnant women with FGR, control group – of 40 healthy pregnant women. The analysis of the intestinal microbiota in 53 pregnant women (25 pregnant women with late FGR and 28 healthy pregnant women) was carried out by using nitrogenous base sequencing in the 16S ribosomal RNA (rRNA) genes.Results. Analyses of the gut microbiome composition in pregnant women in study groups revealed significant differences particularly showing a 1.0 % increase in the proportion of Clostridiales Ruminococcaceae Oscillospira that correlated with a 331.7 g increase in neonatal weight gain; a 1.0 % increase in the level of Coriobacteriales Coriobacteriaceae Collinsella was associated with a 476.2 g decrease in neonatal weight gain. The Firmicutes/Bacteroides ratio was 2,0 and 3.6 in main and control group (p = 0.02), respectively, which may suggest about a microbiological dysbiosis with putative pathophysiological outcomes. A significant association between elevated leucocyte counts (in the absence of other manifestations of inflammatory processes) and the probability of FGR development was found. A white blood cell count ≥ 11.05×109/L predicted the risk of FGR with a sensitivity of 60.6 % and a specificity of 79.2 %. Also, erythrocyte sedimentation rate level of ≥ 41.5 mm/hour was associated with increased FGR risk, demonstrating a sensitivity of 85.7 % and specificity of 70.6 %.Conclusion. It can be concluded that gut dysbiosis may play a role in development of late idiopathic FGR. A negative correlation between a decreased Firmicutes/Bacteroides ratio as well as higher Actinobacteria proportion and fetal birth weight was found.
O pierwszych powieściach Teodora Tomasza Jeża (z rzutem oka na twórczość pisarza w ogóle). Kilka propozycji badawczych
Authors: Radowska-Lisak Mirosława 
Published: January 2025

Artykuł służy przypomnieniu twórczości Teodora Tomasza Jeża (Zygmunta Miłkowskiego) i osadzeniu jej zarówno w kontekście literatury i kultury XIX wieku, jak i w obrębie zainteresowań współczesnej humanistyki. Główna uwaga skoncentrowana jest na pierwszych powieściach Jeża: Wasylu Hołubie (1858) i Handzi Zahornickiej (1859), lecz stanowią one zarazem punkt wyjścia do dyskusji na temat oryginalności i żywotności prozy Miłkowskiego w ogóle. Sformułowanie postulatów badawczych potraktować można jako inspirację do dalszych badań, które w efekcie prowadziłyby do nowych interpretacji i szczegółowych analiz porównawczych.
Application of Elastomer Microsphere Particles as Water-Based Drilling Fluid Lost Circulation Material
Authors: Yang Lili , Ou Zhiting , Chang Xiangyang , Wu Jiale , Chen Haozhe , Jiang Guancheng , Ma Jiaying , Dong Tengfei , Wu Yunpeng , Yu Changjiang , Feng Shangjiang 
Published: January 2025

Summary
Lost circulation is a common issue during oil and gas well drilling, presenting significant safety hazards and resulting in substantial economic losses. Bridging materials typically exhibit limited deformation and poor adaptability to formation pores and fractures, primarily due to size mismatching. Polymer gel materials have the potential to address these issues effectively. While strength is a crucial factor affecting plugging performance, other properties—such as the toughness and elasticity of polymer gel particles—are often overlooked. In this research, we introduce a novel elastomer plugging material that exhibits high toughness and elasticity. The material is composed of polyampholytes, which are synthesized through the copolymerization of an acrylate monomer with long alkyl side groups and a pair of oppositely charged monomers. The acrylic monomer enhances the mobility of the polymer chain, while the oppositely charged monomer generates a gradient interaction, resulting in the formation of ionic aggregates with a broad range of sizes and stiffness. The multigradient strong interaction affords the elastomer remarkable strength and elasticity, which, in turn, safeguards the structural integrity of microsphere particles when subjected to elevated pressure and shear stress. Additionally, the presence of weak interactions, enabled by high mobility, promotes gradual energy dissipation, ultimately enhancing the toughness of the material. PABD [poly (AMPS-BXSZ-DMDAAC)] microsphere particles possess the ability to effectively plug leakage channels by infiltrating pore cracks that are smaller than their own size. Additionally, they can form an elastomer block layer to seal cracks that are larger than their own size, which is facilitated by self-healing through the accumulation of particles and the intermolecular forces between them. As the pressure increases, the plugging layer compresses and becomes more dense, thereby effectively preventing the leakage of drilling fluid into the formation. The experimental results demonstrate that PABD microsphere particles effectively obstruct 8-darcy and 180-darcy consolidated sand trays at temperatures as high as 150°C. Moreover, these particles can be used to seal unconsolidated sand beds of 40−60 mesh, and their plugging performance is significantly enhanced when combined with traditional rubber. This study emphasizes the considerable application potential of elastomer materials in drilling operations.
Lung tumoroids as a testing platform for precision CAR T-cell therapy
Authors: Schmueck-Henneresse Michael , Ehlen Lukas , Farrera-Sal Martí , Szyska Martin , Arndt Janine , Schallenberg Simon , Scholz Cedric , Yang Mingxing , Vollbrecht Claudia , Löwa Anna , Friedrich Rebecca , Mai Marco , Peter Lena , Picht Samira , Schulenberg Sarah , Geray Daniel , Korus Gabriela , Sommerfeld Anke , Treue Denise , Strauchmann Julia , Elsner Aron , Kath Jonas , Vallone Valeria Fernandez , Joosten Maria , Klatte-Schulz Franka , Petersen Ansgar , Stachelscheid Harald , Wagner Dimitrios , Spies Claudia , Rückert Jens-Carsten , Hocke Andreas , Polansky Julia , Stark Regina , Klein Oliver 
Published: January 2025

Abstract

Lung cancer, the leading cause of cancer-related mortality, presents major challenges for both standard-of-care therapies and innovative treatments like CAR T cells due to tumor heterogeneity and resistance. To aid in personalized treatment decisions, preclinical models that incorporate these patient-specific factors are essential. In this study, we developed a platform using matched lung tumoroids and healthy lung organoids. The tumoroids retained the identity of the original tumors, as confirmed by genomic, epigenomic, histological, and proteomic analyses. These tumoroids accurately replicated individual patient responses to standard-of-care therapies, highlighting their predictive value. Our platform enabled the identification of individual responses to CAR T cells and associated mechanisms, indicating that efficacy may be influenced by immunoregulatory factors such as autophagy, IL-8 secretion, and PDL1 overexpression. Our modeling strategy shows promise for guiding patient-specific treatment decisions and may facilitate the preselection of lung cancer patients for CAR T-cell therapy.