bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-life monocentric experience of venetoclax-based regimens for acute myeloid leukemia
Authors: Sciumè Mariarita , Bosi Alessandro , Canzi Marta , Ceparano Giusy , Serpenti Fabio , De Roberto Pasquale , Fabris Sonia , Tagliaferri Elena , Cavallaro Francesca , Onida Francesco , Fracchiolla Nicola Stefano 
Published: March 2023

IntroductionCombination of venetoclax and hypomethylating agents (HMAs) has become a standard of care in acute myeloid leukemia (AML) aged &gt;75 years or who have comorbidities that preclude intensive induction chemotherapy.MethodsWe conducted a monocentric retrospective analysis on adult patients affected by treatment-naïve AML not eligible for standard induction therapy or refractory/relapsed (R/R) AML treated with venetoclax combinations outside clinical trials. Venetoclax was administered at the dose of 400 mg/daily after a short ramp-up and reduced in case of concomitant CYP3A4 inhibitors.ResultsSixty consecutive AML were identified. Twenty-three patients (38%) were affected by treatment-naïve AML and 37 (62%) by R/R AML. Median age was 70 years. Among R/R AML 30% had received a prior allogeneic stem cell transplantation (allo-HSCT). In combination with venetoclax, 50 patients (83%) received azacitidine. Antifungal prophylaxis was performed in 33 patients (55%).Overall response rate was 60%, with 53% of complete remission (CR; 78% for treatment-naïve and 49% for R/R, p 0.017). Median overall survival was 130 days for R/R patients and 269 days for treatment-naïve patients; median event free survival was 145 days for R/R cohort and 199 days for treatment-naïve AML.Measurable residual disease was negative in 26% of evaluable patients in CR/CR with incomplete hematologic recovery after 2 cycles and in 50% after 4 cycles, with no significant association with survival.Eleven patients (18%) received an allo-HSCT after venetoclax combinations. Most common grade 3/4 adverse events were infectious (51% of the patients), or hematological without infections (25% of the patients). Use of CYP3A4 inhibitors was associated with a trend to shorter cytopenias and with a lower rate of infections. Invasive fungal infections were less frequent among patients receiving azole prophylaxis (6% vs 26%; p 0.0659).DiscussionVenetoclax-based regimens are a viable option for AML considered not eligible for standard induction therapy and a valid rescue therapy in the R/R setting.Azole prophylaxis did not significantly affect response and it was associated with a lower rate of invasive fungal infections. Despite a limited number of patients, the association of venetoclax and HMAs proved to be also a feasible bridging therapy to transplantation.
Serum neutralizing capacity and T-cell response against the omicron BA.1 variant in seropositive children and their parents one year after SARS-CoV-2 infection
Authors: Seidel Alina , Jacobsen Eva-Maria , Fabricius Dorit , Class Magdalena , Zernickel Maria , Blum Carmen , Conzelmann Carina , Weil Tatjana , Groß Rüdiger , Bode Sebastian F. N. , Renk Hanna , Elling Roland , Stich Maximillian , Kirchhoff Frank , Debatin Klaus-Michael , Münch Jan , Janda Aleš 
Published: March 2023

IntroductionDurability of immune protection against reinfection with SARS-CoV-2 remains enigmatic, especially in the pediatric population and in the context of immune-evading variants of concern. Obviously, this knowledge is required for measures to contain the spread of infection and in selecting rational preventive measures.MethodsHere, we investigated the serum neutralization capacity of 36 seropositive adults and 34 children approximately one year after infection with the ancestral Wuhan strain of SARS-CoV-2 by using a pseudovirus neutralization assay.ResultsWe found that 88.9% of seropositive adult (32/36) and 94.1% of seropositive children (32/34) convalescents retained the neutralizing activity against the SARS-CoV-2 Wuhan strain (WT). Although, the neutralization effect against Omicron BA.1 (B.1.1.529.1) was significantly lower, 70.6% (24/34) of children and 41.7% (15/36) of adults possessed BA.1 cross-neutralizing antibodies. The spike 1 (S1)-specific T cell recall capacity using an activation-induced marker assay was analyzed in 18 adults and 16 children. All participants had detectable S1-specific CD4 T cells against WT, and 72.2% (13/18) adults and 81,3% (13/16) children had detectable S1 WT-specific CD8 T cells. CD4 cross-reactivity against BA.1 was demonstrated in all investigated adults (18/18), and 66.7% (12/18) adult participants had also detectable specific CD8 BA.1 T cells while we detected BA.1 S1 reactive CD4 and CD8 T cells in 81.3% (13/16) children.DiscussionTogether, our findings demonstrate that infection with the ancestral strain of SARS-CoV-2 in children as well as in adults induces robust serological as well as T cell memory responses that persist over at least 12 months. This suggests persistent immunological memory and partial cross-reactivity against Omicron BA.1.
Treatment with paeoniflorin increases lifespan of Pseudomonas aeruginosa infected Caenorhabditis elegans by inhibiting bacterial accumulation in intestinal lumen and biofilm formation
Authors: Wang Yuxing , Zhang Le , Yuan Xiaoan , Wang Dayong 
Published: March 2023

Paeoniflorin is one of the important components in Paeoniaceae plants. In this study, we used Caenorhabditis elegans as a model host and Pseudomonas aeruginosa as a bacterial pathogen to investigate the possible role of paeoniflorin treatment against P. aeruginosa infection in the host and the underlying mechanisms. Posttreatment with 1.25–10 mg/L paeoniflorin could significantly increase the lifespan of P. aeruginosa infected nematodes. After the infection, the P. aeruginosa colony-forming unit (CFU) and P. aeruginosa accumulation in intestinal lumen were also obviously reduced by 1.25–10 mg/L paeoniflorin treatment. The beneficial effects of paeoniflorin treatment in increasing lifespan in P. aeruginosa infected nematodes and in reducing P. aeruginosa accumulation in intestinal lumen could be inhibited by RNAi of pmk-1, egl-1, and bar-1. In addition, paeoniflorin treatment suppressed the inhibition in expressions of pmk-1, egl-1, and bar-1 caused by P. aeruginosa infection in nematodes, suggesting that paeoniflorin could increase lifespan of P. aeruginosa infected nematode by activating PMK-1, EGL-1, and BAR-1. Moreover, although treatment with 1.25–10 mg/L paeoniflorin did not show obvious anti-P. aeruginosa activity, the P. aeruginosa biofilm formation and expressions of related virulence genes (pelA, pelB, phzA, lasB, lasR, rhlA, and rhlC) were significantly inhibited by paeoniflorin treatment. Treatment with 1.25–10 mg/L paeoniflorin could further decrease the levels of related virulence factors of pyocyanin, elastase, and rhamnolipid. In addition, 2.5–10 mg/L paeoniflorin treatment could inhibit the swimming, swarming, and twitching motility of P. aeruginosa, and treatment with 2.5–10 mg/L paeoniflorin reduced the cyclic-di-GMP (c-di-GMP) level. Therefore, paeoniflorin treatment has the potential to extend lifespan of P. aeruginosa infected hosts by reducing bacterial accumulation in intestinal lumen and inhibiting bacterial biofilm formation.
Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium
Authors: Mi Yaolei , He Ruikun , Wan Huihua , Meng Xiangxiao , Liu Di , Huang Wenjun , Zhang Yanjun , Yousaf Zubaida , Huang Hongwen , Chen Shilin , Wang Ying , Sun Wei 
Published: March 2023

IntroductionFlower color is an ideal trait for studying the molecular basis for phenotypic variations in natural populations of species. Epimedium (Berberidaceae) species exhibit a wide range of flower colors resulting from the varied accumulation of anthocyanins and other pigments in their spur-like petals and petaloid sepals.MethodsIn this work, the anthocyanidins of eight different Epimedium species with different floral pigmentation phenotypes were analyzed using HPLC. Twelve genes involved in anthocyanin biosynthesis were cloned and sequenced, and their expression was quantified.ResultsThe expression levels of the catalytic enzyme genes DFR and ANS were significantly decreased in four species showing loss of floral pigmentation. Complementation of EsF3’H and EsDFR in corresponding Arabidopsis mutants together with overexpression of EsF3’5’H in wild type Arabidopsis analysis revealed that these genes were functional at the protein level, based on the accumulation of anthocyanin pigments.DiscussionThese results strongly suggest that transcriptional regulatory changes determine the loss of anthocyanins to be convergent in the floral tissue of Epimedium species.
The moderating role of spirituality and gender in Canadian and Iranian emerging adolescents’ theory of mind and prosocial behavior
Authors: Khalili Nadia , Bosacki Sandra , Talwar Victoria 
Published: March 2023

IntroductionWhile research has found a link between ToM and prosociality in terms of caring and helping others which may also vary across cultures, the moderating role of spirituality and culture of this association in emerging adolescence has received little attention.MethodsThe current study empirically “examined” the role of spirituality and gender in relation to ToM and prosocial behavior in Canadian and Iranian emerging adolescents. A total of 300 (153 girls) emerging adolescents (M = 11.502, SD = 2.228) were recruited from Montreal, Canada and Karaj, Iran. A series of double moderation analysis and ANOVA was conducted.Results and discussionResults indicated the difference between direct and indirect influences of ToM and its interactions with culture, gender, and spirituality on prosocial behavior. This implies an emerging complex framework which suggests the dynamic nonlinear interactions between these factors. Implications for youth’s social-emotional understanding will be discussed.
The relationship between urinary selenium levels and risk of gestational diabetes mellitus: A nested case–control study
Authors: Liu Yuanxia , Chen Hongmei , Zhang Mengtian , Zhu Gangjiao , Yang Yan , Li Yuanyuan , Lu Wei , Zhang Hongling 
Published: March 2023

BackgroundSelenium (Se) is an essential trace element for the human body. Serum Se and urinary Se are also biomarkers to assess Se exposure status. However, studies focusing on the association between urinary Se and the risk of gestational diabetes mellitus (GDM) are rare.ObjectiveTo investigate the association between urinary Se and the risk of GDM.MethodsA nested case–control study based on a prospective birth cohort in Wuhan, China, which focuses on the effects of prenatal environmental factors exposure on pregnant women and children’s health was conducted. Two hundred and twenty-six cases and 452 controls were included. Maternal urine samples were collected before GDM diagnosis, and the urinary Se levels were determined. We assessed the association of urinary Se with GDM by conditional logistic regression with maternal urinary Se level as a categorical variable, and estimated the association between Se and glucose levels by multiple linear regression. The potential modifier roles of maternal age and fetal sex have also been assessed.ResultsLower urinary level of Se was significantly associated with a higher risk of GDM (OR = 2.35 for the tertile 1, 95% CI:1.36-4.06; adjusted OR = 1.79 for the tertile 2, 95%CI:1.09-2.95; p for trend = 0.01). Fetal sex had an interaction with Se in the association with GDM. The association was more pronounced among pregnant women with female fetuses than with male fetuses.DiscussionOur study suggested a significant negative association between urinary Se and the risk of GDM, and this association may vary depending on the fetal sex.
Radiological outcomes of PEEK rods in patients with lumbar degenerative diseases: A minimum 5-year follow-up
Authors: Huang Weimin , Wang Wenqiao , Xu Xiaoduo , Wang Lei , Wang Jingming , Yu Xiuchun 
Published: March 2023

PurposeTo determine the long-term radiological outcomes of PEEK rods in patients with lumbar degenerative diseases.MethodsRadiological outcomes of cohort cases with lumbar degenerative diseases following PEEK rods were retrospectively studied. Disc height index (DHI) and range of motion (ROM) were measured by x-rays. The CT scans and reconstruction were used to determine screw breakage, rods fracture, screw loosening and intervertebral bony fusion status. The MRI scans were used to evaluate the changes of intervertebral discs at the non-fusion segments and adjacent segments in terms of Pfirrmann Classification.ResultsA total of 40 patients completed the mean of 74.8 ± 9.6 months follow-up, with 32 patients undergoing hybrid surgery and 8 patients undergoing non-fusion surgery. The mean DHI changed from preoperative 0.34 to 0.36 at the final follow-up and the ROM declined from 8.8° preoperatively to 3.2° at the final visit, however, both had no statistical differences. Of the 40 levels underwent non-fusion procedure, 9 levels showed disc rehydration with 7 patients from Grade 4 to Grade 3 and 2 patients from Grade 3 to Grade 2. The other 30 cases did not show distinctive change. No screw loosening or rods breakage were detected during the follow-up periods.ConclusionPEEK rods have obvious protective effects on degenerated intervertebral disc of non-fusion segments and the incidence of complications related to internal fixation is low. PEEK rods pedicle screw system is safe and effective in the treatment of lumbar degenerative diseases.
Assessment of New and Genome-Reduced Pseudomonas Strains Regarding Their Robustness as Chassis in Biotechnological Applications
Authors: Cárdenas Espinosa María José , Schmidgall Tabea , Pohl Jessica , Wagner Georg , Wynands Benedikt , Wierckx Nick , Heipieper Hermann J. , Eberlein Christian 
Published: March 2023

Organic olvent-tolerant strains of the Gram-negative bacterial genus Pseudomonas are discussed as potential biocatalysts for the biotechnological production of various chemicals. However, many current strains with the highest tolerance are belonging to the species P. putida and are classified as biosafety level 2 strains, which makes them uninteresting for the biotechnological industry. Therefore, it is necessary to identify other biosafety level 1 Pseudomonas strains with high tolerance towards solvents and other forms of stress, which are suitable for establishing production platforms of biotechnological processes. In order to exploit the native potential of Pseudomonas as a microbial cell factory, the biosafety level 1 strain P. taiwanensis VLB120 and its genome-reduced chassis (GRC) variants as well as the plastic-degrading strain P. capeferrum TDA1 were assessed regarding their tolerance towards different n-alkanols (1-butanol, 1-hexanol, 1-octanol, 1-decanol). Toxicity of the solvents was investigated by their effects on bacterial growth rates given as the EC50 concentrations. Hereby, both toxicities as well as the adaptive responses of P. taiwanensis GRC3 and P. capeferrum TDA1 showed EC50 values up to two-fold higher than those previously detected for P. putida DOT-T1E (biosafety level 2), one of the best described solvent-tolerant bacteria. Furthermore, in two-phase solvent systems, all the evaluated strains were adapted to 1-decanol as a second organic phase (i.e., OD560 was at least 0.5 after 24 h of incubation with 1% (v/v) 1-decanol), which shows the potential use of these strains as platforms for the bio-production of a wide variety of chemicals at industrial level.
Accuracy Assessment and Validation of Multi-Source CHIRPS Precipitation Estimates for Water Resource Management in the Barada Basin, Syria
Authors: Alsilibe Firas , Bene Katalin , Bilal Ghada , Alghafli Khaled , Shi Xiaogang 
Published: March 2023

The lack of sufficient precipitation data has been a common problem for water resource planning in many arid and semi-arid regions with sparse and limited weather monitoring networks. Satellite-based precipitation products are often used in these regions to improve data availability. This research presents the first validation study in Syria for Climate Hazards Group InfraRed Precipitation with Stations (CHIRPS) estimates using in-situ precipitation data. The validation was performed using accuracy and categorical statistics in the semi-arid Barada Basin, Syria, between 2000 and 2020. Multiple temporal scales (daily, pentad, monthly, seasonally, and annual) were utilized to investigate the accuracy of CHIRPS estimates. The CHIRPS results indicated advantages and disadvantages. The main promising result was achieved at the seasonal scale. Implementing CHIRPS for seasonal drought was proven to be suitable for the Barada Basin. Low bias (PBwinter = 2.1%, PBwet season = 12.7%), high correlation (rwet season = 0.79), and small error (ME = 4.25 mm/winter) support the implementation of CHIRPS in winter and wet seasons for seasonal drought monitoring. However, it was observed that CHIRPS exhibited poor performance (inland pentads) in reproducing precipitation amounts at finer temporal scales (pentad and daily). Underestimation of precipitation event amounts was evident in all accuracy statistics results, and the magnitude of error was higher with more intense events. CHIRPS results better corresponded in wet months than dry months. Additionally, the results showed that CHIRPS had poor detection skill in drylands; on average, only 20% of all in-situ precipitation events were correctly detected by CHIRPS with no effect of topography found on detection skill performance. This research could be valuable for decision-makers in dryland regions (as well as the Barada Basin) for water resource planning and drought early warning systems using CHIRPS.
Magie théurgique, angélologie et vision béatifique dans le Liber sacratus sive juratus attribué à Honorius de Thèbes
Authors: Boudet Jean-Patrice 
Published: March 2023

Inspiré à la fois par la magie juive et par la théurgie chrétienne de l’ars notoria,
le Liber sacratus sive juratus, attribué fictivement à Honorius de Thèbes, a pour principal objectif de donner ici-bas à son adepte la vision béatifique. Conçu peut-être à l’origine comme un simple manuel de magie rituelle, ce texte s’en écarte nettement par sa dimension théurgique et il comprend un prologue qui constitue une attaque en règle contre la papauté et les cardinaux, accusés d’être inspirés par le Diable lorsqu’ils s’en prennent aux magiciens. L’on peut donc se demander s’il n’a pas été composé, dans la version qui a été conservée, en réponse aux prises de position de Jean XXII sur la magie et sur la vision béatifique graduelle. Visé clairement par les condamnations parisiennes de 1398, il représente par ailleurs un précieux témoin de ce que pouvait être l’angélologie et la démonologie magiques de la fin du Moyen Âge.
Le origini del concetto di feudo come istituto giuridico
Authors: Brancoli Busdraghi Piero 
Published: March 2023

Lo scritto ha per tema la genesi della nozione di «feudo » nel senso preciso, rigorosamente giuridico, che fu proprio del termine sino alla fine del XVIII secolo : quello cioè di una concessione reale tipica, qualificata e condizionata da uno specifico comportamento (fedeltà ed eventualmente servizio) dovuto dal concessionario (vassallo) nei riguardi del concedente (signore). Il concetto perviene a compiuta maturazione intorno alla metà del XII secolo a seguito dell’elaborazione, avutasi nella giurisprudenza e nella consuetudine, delle norme emanate con l’Edictum de beneficiis dell’imperatore Corrado II sulla materia degli stipendi (beneficia, feuda) soliti a elargirsi alla militia del Regno Italico. Tali stipendi non hanno alcun rapporto con le concessioni reali fondiarie praticate a quell’epoca, ma consistono in assegnazioni di denaro o derrate precariamente imputate a determinati immobili, secondo una prassi generalmente seguita per ogni sorta di servizio. Le statuizioni dell’editto decisive per l’individualizzazione della categoria dei feudi militari riguardarono l’irrevocabilità dell’assegnazione, il divieto di trasferire lo stipendio da un immobile a un altro e il rinnovo dell’assegnazione al figlio del miles
defunto. Tali statuizioni, in seguito arricchite e modificate in via negoziale e giurisprudenziale di pari passo con il declino dell’importanza del servizio (svolto in antico in modo continuato), si risolsero nel XII secolo nella comparsa del «feudo » come specifica concessione reale qualificata dalla semplice fedeltà, ossia dall’obbligo di non ledere i diritti e gli interessi del signore concedente e di aiutare quest’ultimo in situazioni di pericolo. La nuova figura così emersa, che venne teorizzata sul piano dogmatico da Oberto dall’Orto e da Pillio, si rivelò in campo politico, in molteplici situazioni, come un utile strumenti di raccordo fra i centri di potere locali e l’autorità di superiori ordinamenti statuali o egemonici.
Diagnostic limitations of lung fiber counts in asbestos-related diseases
Authors: Schneider Joachim , Arhelger Rolf , Brückel Bernd , Baur Xaver 
Published: March 2023

Background Lung dust fibre analyses have been used by some pathologists to estimate past asbestos exposure in the workplace and its related health risks. Asbestos, however, especially the predominately applied chrysotile asbestos type, undergoes translocation, clearance and degradation in the lungs. Objectives We quantified the asbestos fibre and ferruginous (asbestos) body (FB) content in human tissue with respect to the German asbestos ban in 1993 and the interim period of more than 20 years in order to evaluate the diagnostic evidence of these analyses for asbestos-related diseases (ARD). Methods Lung dust analyses have been used in empirical assessments of ARD since 1982. Tissue samples of about 2 cm3 were used and processed in standardized manner. FB was analysed by light microscopy and asbestos fibres by scanning transmission electron microscopy (STEM). Results Chrysotile and amphibole fibre concentrations in the lung tissue depend roughly on the cumulative asbestos exposure levels in the workplace. However, the concentration of lung asbestos fibre and FB depends on the year of examination and especially on the interim period. As the interim period increases, the asbestos fibre burden decreases. There is no relationship between FB and chrysotile asbestos fibre concentrations and only a weak correlation between FB and crocidolite fibre concentrations. There was no significant difference in chrysotile and amphibole fibre concentrations as well as in FB counts between the different ARD. Conclusions Due to the length of interim periods, a past exposure to chrysotile or amphibole asbestos can no longer be detected with FB or asbestos fibre measurement in lung tissue. This means that negative results of such measurements cannot disprove a qualified occupational case history of asbestos exposures and the related health risks due to the fibrogenic and carcinogenic potential of asbestos.
Geometry-based Stochastic Channel Model for Vehicle-to-vulnerable Road Users Communications in Critical Scenarios - Part I: Parameterization
Authors: Rashdan Ibrahim , de Ponte Muller Fabian , Sand Stephan , Caire Giuseppe 
Published: March 2023

<p>This work presents the first complete parameterization for WINNER-type geometry-based stochastic channel model (GSCM) at 5.2 GHz based on channel measurements. In order to parameterize the GSCM channel model, the large scale parameters (LSPs) and their correlations are estimated in the log domain. The results show that the log-normal distribution provides a good fit to the distributions of the LSPs. The proposed model parameterization can be used to generate synthetic channel instances which are required to develop and evaluate V2VRU communication systems and collision avoidance algorithms in critical accident scenarios.</p>