bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial inoculants alter resilience towards drought stress in wheat plants
Authors: Singh Madhulika , Sharma Jai Gopal , Giri Bhoopander 
Published: June 2023

Abstract
The present study aimed to investigate the role of Piriformospora indica, arbuscular mycorrhiza fungi (AMF), and plant growth-promoting bacteria (PGPB) in alleviating drought stress in the HD-2967 wheat cultivar. In a completely randomized design experiment, plants were subjected to different water regimes of 75 and 35% field capacity (FC) under greenhouse conditions. Under different water regimes, microbial inoculation significantly enhanced the morphological, physico-biochemical, and ultrastructural characteristics of the wheat plants. Plants inoculated with PGPB, P. indica, and AMF showed increased shoot and root length, shoot and root biomass, leaf area, photosynthetic rate, transpiration rate, stomatal conductance, and internal CO2 as compared to uninoculated plants under all water regimes. The PGPB, P. indica, and AMF-inoculated wheat plants accumulated higher content of glycine betaine, total sugars, trehalose, proline, putrescine, spermidine, carotenoids, proteins, α-tocopherol, and a decrease in lipid peroxidation, relative membrane permeability, and lipoxygenase enzyme (LOX) activity as compared to uninoculated plants. Besides, microbes-inoculated wheat plants showed a higher level of antioxidant enzymes viz., superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) than uninoculated plants. Microbial inoculation helped wheat plants to overcome water stress-induced deficiency of macro- (Ca2+, Mg2+, and K+) and micronutrient (Cu, Mn2+, Fe, and Zn2+), and reduced damage to the cell ultrastructure (plasma membrane and chloroplasts). Comparing the potential of microbial inoculants to increase growth and nutritional, biochemical, physiological, and ultrastructural changes, the PGPB-inoculated wheat plants showed greater drought resilience followed by AMF and P. indica inoculated plants. These microbial inoculants offer a significant potential to meet the challenges of sustainable agriculture under drought conditions.
Application of seismic attribute analysis techniques to multi-scale nature fractures description: A case study of the Longmaxi-Wufeng formation in H shale gas block, Yangtze plate, China
Authors: Xie Qinghui , Han Guosheng , Chen Junhao , Zhao Chunduan , Li Qingshan , Guan Shoujun 
Published: June 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Agreement and Precision of Wide and Cube Scan Measurements between Swept-source and Spectral-domain OCT in Normal and Glaucoma Eyes
Authors: Hou Huiyuan , EI-Nimri Nevin W. , Durbin Mary K. , Arias Juan D. , Moghimi Sasan , Weinreb Robert N. 
Published: June 2023

Abstract
This study aimed to evaluate agreement of Wide scan measurements from swept-source optical coherence tomography(SS-OCT) Triton and spectral-domain OCT(SD-OCT) Maestro in normal/glaucoma eyes, and to assess the precision of measurements from Wide and Cube scans of both devices. Three Triton and three Maestro operator/device configurations were created by pairing three operators, with study eye and testing order randomized. Three scans were captured for Wide (12mm×9mm), Macular Cube (7mmx7mm–Triton; 6mmx6mm-Maestro), and Optic Disc Cube (6mmx6mm) scans for 25 normal eyes and 25 glaucoma eyes. Thickness of circumpapillary retinal nerve fiber layer(cpRNFL), ganglion cell layer+inner plexiform layer(GCL+), and ganglion cell complex(GCL++) was obtained from each scan. A two-way random effect analysis of variance model was used to estimate the repeatability and reproducibility; agreement was evaluated by Bland-Altman analysis and Deming regression. Precision limit estimates were low: <5µm for macular and <10µm for optic disc parameters. Precision for Wide and Cube scans of both devices were comparablein both groups. Excellent agreement between the two devices was found for Wide scans, with the mean difference<3µm across all measurements (cpRNFL<3µm, GCL+<2µm, GCL++<1µm), indicating interoperability. A single Wide scan covering the peripapillary and macular regions may be useful for glaucoma management.
Adjuvant chemotherapy in pathological node-negative non-small cell lung cancer
Authors: Cheng Ya-Fu , Chen Yi-Ling , Liu Chia-Chi , Lin Ching-Min , Tong Shao-Syuan , Wang Bing-Yen 
Published: June 2023

Abstract
Non–small cell lung cancer (NSCLC) is associated with a poor survival rate, even for patients with early-stage cancer. Identifying patients with pathological N0 NSCLC who may benefit from adjuvant chemotherapy treatment after surgery is essential. We conducted a retrospective cohort study used data from the Surveillance, Epidemiology, and End Results database and included 26,380 patients with pathological N0 NSCLC after surgery between January 2018, and December 2019. Among 26,380 patients, 24,273 patients received surgery alone and the other 2,107 patients received surgery plus adjuvant chemotherapy. After 1:1 propensity score matching, both groups contained 2,107 patients. Adjuvant chemotherapy did not show significantly better 24-month survival in T2aN0 NSCLC patients (83.41% vs. 78.79%, p = 0.056), although it did for T2bN0 patients (84.07% vs. 66.93%, p < 0.001). Poorly-differentiated NSCLC remained a high-risk factor for pT2N0, and adjuvant chemotherapy provided better 24-month survival after matching (86.36% vs. 77.51%, p = 0.003). In conclusion, when treating pN0 NSCLC, adjuvant chemotherapy had a beneficial effect when the tumor size was larger than 4 cm. The effect when the tumor size was between 3 cm and 4 cm was not remarkable. Poorly-differentiated NSCLC was a high-risk factor in the pT2N0 stage.
Patient transfer patterns and the spread of hospital-acquired infections in subnetworks of a country: an example of federal states in Germany
Authors: Xia Hanjue , Horn Johannes , Piotrowska Monika J. , Sakowski Konrad , Karch André , Kretzschmar Mirjam , Mikolajczyk Rafael 
Published: June 2023

Abstract
Pathogens typically responsible for hospital-acquired infections (HAIs) constitute a major threat to healthcare systems worldwide. They spread via hospital (or hospital-community) networks by readmissions or patient transfers. Therefore, knowledge of these networks is essential to develop and test strategies to mitigate and control the HAI spread. Until now, no methods for comparing healthcare networks across different systems were proposed. Based on healthcare insurance data from four German federal states (Bavaria, Lower Saxony, Saxony and Thuringia), we constructed hospital networks and compared them in a systematic approach regarding population, hospital characteristics, and patient transfer patterns. Direct patient transfers between hospitals had only a limited impact on HAI spread. Whereas, with low colonization clearance rates, readmissions to the same hospitals posed the biggest transmission risk of all inter-hospital transfers. We then generated hospital-community networks, in which patients either stay in communities or in hospitals. We found that network characteristics affect the final prevalence and the time to reach it. However, depending on the characteristics of the pathogen (colonization clearance rate and transmission rate or even the relationship between transmission rate in hospitals and in the community), the studied networks performed differently. The differences were not large, but justify further studies.
WebRTC over 5G: A Study of Remote Collaboration QoS in Mobile Environment
Authors: Nakazato Jin , Tsukada Manabu , Nakagawa Kousuke , Ito Koki , Fontugne Romain , Esaki Hiroshi 
Published: June 2023

Abstract
The increasing demand for remote collaboration and remote working has become crucial to daily life owing to the Covid-19 pandemic and the development of internet-based video distribution services. Furthermore, low-latency remote collaboration, such as teleoperation and support applications designed for in-vehicle environments, has gained considerable attention. The 5G technology is considered as a key infrastructure for remote collaboration. This study aimed to evaluate the actual 5G capability to achieve high quality of service (QoS) for remote collaboration. We designed and implemented a measurement tool to monitor the QoS of remote collaboration under real-world 5G conditions. We performed measurements encompassing the various 5G frequency bands. During these experiments, we employed various tools to obtain detailed mobile signal conditions to analyze the relationship between various environmental factors (e.g. signal quality, band, handoff, geographic conditions, and mobility) and the QoS performance of remote collaboration in a real-world 5G environment. This study elucidated the correlation between the WebRTC performance and various environmental factors as well as the performance improvement potential by leveraging the communication technologies of multiple mobile carriers. The collected data has been made publicly available to foster research on QoS and 5G.
Blind 2-Microphone Acoustic Noise Reduction Algorithms Using Efficient Variable Step-Size Adapted by Minimizing the Intercorrelation Function
Authors: BENDOUMIA Redha 
Published: June 2023

Abstract
Recently, there have been advancements in adaptive reduction of noise signal using 2-microphones adaptive algorithms. Specifically, the normalized form of least-mean-square algorithm (NLMS) with fixed-step-size parameters (FS) has been combined with direct-and-recursive structures of source separation. Compared to conventional one-microphone methods, these combinations provide speech quality superiority. However, the main limitation of these 2-microphones adapting algorithms (Direct combination: Forward NLMS and Recursive combination: Backward NLMS) is their poor steady state regime with large FS value, while small step-sizes values result a slow speed of convergence. To address these issues, we presented in this study a new step-size approach (VS) based the intercorrelation function minimization in the time domain for the basic FNLMS and BNLMS algorithms. Our approach is proposed exactly to obtain an optimal value of VS parameters by intercorrelation minimization among the enhanced signal and noisy microphone ones. The proposition optimization improves both the steady state values and convergence speed simultaneously. The proposed 2-microphones adapting algorithms were evaluated through simulations conducted in highly noisy environments, using the system of mismatch criterion and output estimation of signal-to-noise ratio ones. The comparative simulations results confirmed that our approach outperforms existing methods in terms of effectiveness and efficiency.
A quantization of the Loday-Ronco Hopf algebra
Authors: Esteves João N. 
Published: June 2023

Abstract
We propose a quantization algebra of the Loday-Ronco Hopf algebra $k[Y^\infty]$, based on the Topological Recursion formula of Eynard and Orantin. We have shown in previous works that the Loday-Ronco Hopf algebra of planar binary trees is a space of solutions for the genus 0 version of Topological Recursion, and that an extension of the Loday Ronco Hopf algebra as to include some new graphs with loops is the correct setting to find a solution space for arbitrary genus. Here we show that this new algebra $k[Y^\infty]_h$ is still a Hopf algebra that can be seen in some sense to be made precise in the text as a quantization of the Hopf algebra of planar binary trees, and that the solution space of Topological Recursion is a sub-algebra $\mathcal{A}^h_{\text{TopRec}}\subset k[Y^\infty]_h$ that nevertheless doesn't inherit the Hopf algebra structure. We end the paper with a discussion on the cohomology of $\mathcal{A}^h_{\text{TopRec}}$ in low degree.
Potent mosquitocidal effect of ivermectin-treated nettings and sprayed walls on Anopheles gambiae
Authors: Hamid-Adiamoh Majidah , Muhammad Abdul Khalie , Assogba Benoit Sessinou , Soumare Harouna Massire , Jadama Lamin , Diallo Moussa , D’Alessandro Umberto , Ndiath Mamadou Ousmane , Erhart Annette , Amambua-Ngwa Alfred 
Published: June 2023

Abstract
Ivermectin (IVM) has been proposed as a new tool for malaria control as it is toxic on vectors feeding on treated humans or cattle. Nevertheless, IVM may have a direct mosquitocidal effect when applied on bed nets or sprayed walls. Laboratory-reared, insecticide-susceptible Kisumu Anopheles gambiae were exposed to IVM on impregnated netting materials and sprayed plastered- and mud walls using cone bioassays. All mosquitoes died within 6 hours when exposed to IVM-treated nettings, and within 18 hours for both sprayed walls. Mosquito mortality rate on the IVM-treated nettings was similar to that of positive control (100% mortality at 2 hour) while survival was significantly longer on both IVM-sprayed walls (100% mortality at 18 hour) (Log rank X2 = 36.28, p<0.001). Adjusted Cox model predicted a significant interaction between IVM treatment and surface type, as well as significantly higher mosquito mortality on IVM-treated nettings [HR=12.92 (95% CI: 7.69-18.14), p<0.001] compared to plastered- and mud walls. IVM also inhibited mosquito blood feeding and oviposition. Our results show a direct mosquitocidal effect of IVM on An. gambiae Kisumu strain and suggest its potential application as a new insecticide for effective vector control strategies.
Prolonged Exposure to Plant Volatiles Does Not Significantly Affect Pban Expression and Mating Behavior in Diamondback Moth [plutella Xylostella (Lepidoptera: Plutellidae)]
Authors: TIAN HOUJUN , XU RUIBIN , LI HENG , LIN SHUO , CHEN YIXIN , CHEN YONG , YANG GUANG , WEI HUI 
Published: June 2023

Abstract
Via plant volatiles, herbivorous insects locate hosts, find food, and release spawn for better survival and reproduction. Moreover, plant volatiles not only regulate the synthesis and release of sex pheromones in insects but also help them in the search and orientation of sources of sex pheromones. However, after prolonged exposure to plant volatiles, the changes mediating the mating behavior of diamondback moth (DBM) [Plutella xylostella (L.) (Lepidoptera: Plutellidae)] are unclear. DBMs were treated with allyl isothiocyanate, a volatile from cruciferous vegetables; however, it did not improve the mating rate and had a limited effect on mating rhythm. Moreover, the treatment inhibited mating behaviors in 3-day-old DBMs and decreased mating time in 5-day-old DBMs. After prolonged exposure to allyl isothiocyanate, the total mating time of DBM was not significantly different from that after prolonged exposure to n-hexane (control). However, the longest mating time after emergence in DBM after prolonged exposure to allyl isothiocyanate was delayed by 1 day compared with the exposure to n-hexane. Prolonged exposure to plant volatiles intensified the response behavior of DBM to sex pheromones; however, the content of Z11-16: Ald, a major component of sex pheromones, in female DBM gonads exhibited no change. Pheromone biosynthesis activating neuropeptide gene (PBAN) was downregulated in DBMs after prolonged exposure to plant volatiles. These findings suggested that prolonged exposure (6 h) to plant-derived volatiles has little effect on the mating behavior of DBM. The study provided practical guidance for applying phytochemicals in pest control by regulating insect behavior.
Sleep, eating habits and socioeconomic changes in children: Effects of the COVID-19 pandemic
Authors: Hermes Fernanda Nascimento , Silva Amanda Caroline Queiroz da , Ribeiro Willian Silva , Bruzi Alessandro Teodoro , de Melo Camila Maria 
Published: June 2023

Abstract
Introduction: The current pandemic scenario has brought consequences for the health of the child population, including changes in sleep and eating behavior.
Objectives: The present study aimed to evaluate changes in sleep, eating habits and socioeconomic status during the pandemic outbreak.
Methods: A longitudinal study with 37 children between 4-10 years old from a public school. Sleep habits were evaluated through the Questionnaire of Sleep Habits for Children (QSTC), food ingestion, through the 24-hour Recall, and collected socioeconomic data.
Results: There was an increase in total sleep time (p<0.01) and a decrease in the overall sleep quality (p<0.01). A reduction in the number of meals consumed was observed (p<0.01). Higher consumption of ultraprocessed food was observed in relation to in natura, with no changes after pandemic. A socioeconomic decline was observed in 43.2% of families (p<0.01).
Conclusion: Improvements in children's sleep quality and duration, a reduction in the number of meals per day and the loss of access to school meals was observed.
WITHDRAWN: Preparation of Lycium barbarum active glycopeptide and investigate its apoptotic effects on melanoma
Authors: Qi Jinghua , Qi Xingli , Huang Dan , Yuan Meng , Xu Xia , Guo Wenlong , Lu Lili , Meng Mingjie , Rao Guirong , Chen Hongyuan , Rui Wen 
Published: June 2023

Abstract
Previous studies have shown that the L. barbarum fruit polysaccharides could inhibit the growth of cancer cells, but the active ingredient and mechanism of melanoma inhibition are unknown. The preparation of L. barbarum active glycopeptide (LBAG) and its composition was studied to investigate its mechanisms of action on melanoma. LBAG from L. barbarum was extracted and isolated using aqueous-alcoholic precipitation and identified by ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry. Then，how LBAG affected the apoptosis and cell cycle of B16 cells was explored by colony formation assay, cell scratch test, flow cytometry, and Western blot. LBAG had a molecular weight of 10-15 kDa and contained Man, Rha, GlcA, Glc, Gal, and Ara as well as 18 amino acids. Treatment with LBAG significantly decreased B16 cell proliferation and induced cycle arrest at phase G0/G1, which was accompanied by the accumulation of reactive oxygen species. Western blot revealed that LBAG induced the apoptosis and cycle arrest of B6 cells by decreasing the phosphorylation of p38-MAPK and Akt, and the effects were inhibited by N-acetyl-L-cysteine. In mouse xenografts, LBAG inhibited tumor growth via p38-MAPK and AKT signaling. LBAG induces apoptosis in B16 cells through ROS-mediated activation of p38-MAPK and Akt signaling pathways.
Establishment and validation of lung adenocarcinoma aging-related genes prognostic model based on TCGA database
Authors: Xing Long , Wu Shuangli , Wu Tiecheng , Xu Jingxuan , Li Xingya 
Published: June 2023

Abstract
Background To explore the differentially expressed aging-related genes (ARGs) in lung adenocarcinoma (LUAD) and develop a prognostic model for LUAD based on aging-related genes.Methods RNA high-throughput transcriptome data of LUAD were downloaded from The Cancer Genome Atlas (TCGA) database, and ARGs were obtained from the Aging Atlas database. A prognostic model for LUAD was constructed based on differentially expressed aging-related genes in LUAD and validated. A nomogram chart and calibration curve were further constructed to explore the clinical application value of the model.Results A total of 80 differentially expressed aging-related genes were obtained by Venn diagram analysis. Seven differentially expressed ARGs with independent prognostic significance were screened by univariate and multivariate Cox regression analysis. LASSO regression analysis was performed on the seven genes to construct a prognostic model for LUAD. Kaplan-Meier survival curve analysis showed that compared with the low-risk group, the high-risk group was significantly associated with poor overall survival (OS) and the difference was statistically significant (P < 0.05). The area under the receiver operating characteristic (ROC) curve for 1, 3, and 5 years were 0.706, 0.725, and 0.642 respectively, indicating that the risk model had high sensitivity and specificity. Univariate and multivariate Cox regression analysis showed that the risk score was an independent prognostic factor for LUAD. The calibration curve and nomogram chart of gene risk score were further constructed, and the overall consistency of the calibration curve nomogram chart was 0.729, indicating that the model had high accuracy in predicting outcomes.Conclusion The risk model constructed based on differentially expressed ARGs in this study can serve as a prognostic feature for LUAD, providing a reference for individualized treatment of LUAD patients.
Influence of the COVID-19 outbreak in vulnerable patients (pediatric patients, pregnant women, and elderly patients) on an emergency medical service system: a pre- and post-COVID-19 pandemic comparative study using the population-based ORION registry
Authors: Ota Koshi , Nitta Masahiko , Komeya Tomonobu , Matsuoka Tetsuya , Takasu Akira 
Published: June 2023

Abstract
Background
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread all over the world.
Objective
To assess the influence of the COVID-19 pandemic on emergency medical services (EMS) for vulnerable patients transported by ambulance.
Methods
This study was a retrospective, descriptive study with a study period from 1 January 2019 to 31 December 2021 using the Osaka Emergency Information Research Intelligent Operation Network (ORION) system. We included all pediatric patients, pregnant women, and elderly patients ≥65 years of age transported by ambulance in Osaka Prefecture. The main outcome of this study was difficult-to-transport cases. We calculated the rate of difficult-to-transport cases under several conditions.
Results
For the two year-long periods of 1 January 2019 to 31 December 2019 and 1 January 2021 to 31 December 2021, a total of 887,647 patients were transported to hospital by ambulance in Osaka Prefecture. The total number of vulnerable patients was 579,815 (304,882 in 2019 and 274,933 in 2021). Multivariate logistic regression analysis showed that difficult-to-transport cases were significantly more frequent in 2021 than in 2019. Difficult-to-transport cases were significantly less frequent in the vulnerable population than in the non-vulnerable population (adjusted odds ratio 0.81, 95% confidence interval 0.80–0.83; P < 0.001).
Conclusion
During the pandemic (2021), difficult-to-transport cases were more frequent compared to pre-pandemic (2019), however, vulnerable patients were not the cause of difficulties in obtaining hospital acceptance for transport.