bluuebunny/crossref_metadata_2025_split huggingface dataset:
rGO-ZnO nanowire deposited filamentous seaweed nanofibrous cellulose for paper supercapacitor
Authors: Bhutiya Priyank . L. , Kapdaia Rahul , Tripathi Brijesh , Sanjaliya Yash , Rasheed M. Abdul , Rao P. L.S. , Hasan S. Zaheer 
Published: February 2023

Abstract
Nanosized architectural (A Spider’s web) structure of cellulose
(Iα ) was extracted from green seaweed Chaetomorpha
antennina by using bleaching treatment. Further, reduced
graphene oxide (rGO) and zinc oxide (ZnO) nanowire deposited over
seaweed cellulose by using simple hydrothermal method. Simple press
method was used to prepare rGO-ZnO seaweed cellulose nanocomposite
for paper supercapacitor. This rGO-ZnO seaweed cellulose paper
anode material was characterized by using various analytical
techniques such as FT-IR, SEM, TGA, XRD and tensile test. XRD
peaks reveals that when graphene oxide powder mixed with seaweed
cellulose, it was reduced and gave Xrd peak of reduced Graphene
oxide (rGO). This paper supercapacitors were tested in CV, GCD and
EIS. From GCD, the specific energy density of ZnO- cellulose
paper device is found to be 0.00066 Wh/kg whereas, for rGO-ZnO
cellulose paper device have greater energy density of 5.21
Wh/kg. From EIS, the series resistance of ZnO- cellulose is found
to be 326 Ω and for ZnO-rGO -cellulose is 2.16 Ω. This marine
resources based rGO-ZnO seaweed cellulose paper supercapacitor have
application in various energy storage domains including electric
vehicles and electronic industries because it is bio-degradable,
cost effective, thinnest, safe to use as well as high
performance.
Phylogenetic relationships of Prunus (Rosaceae) distributed in North East India: insights from the ribosomal internal transcribed spacer sequence (ITS)
Authors: Mekrini Jennifer N. , Thongam Biseshwori 
Published: February 2023

Abstract
Background
Prunus L. is a large genus including trees and shrubs such as apricot, almond, peaches, plum and nectarines belonging to family Rosaceae. The phylogenetic analysis using ITS sequence was conducted to understand the evolutionary relationship between the Prunus sp. of North East India.
Methods and results
The present study focuses on the 32 accessions, comprising of six voucher specimen of Prunus cerasoides, one voucher specimen of Prunus campanulata, one voucher specimen of Prunus napaulensis, one voucher specimen of Prunus domestica and other twenty two voucher specimen of Prunuscomprising 5 subgenera according to Rehder (1940) and one voucher specimen of Pyrus communis (outgroup) archived from GenBank. The molecular marker (ITS) was utilized in the present study to understand the phylogenetic relationship. Various molecular markers are utilized in assessing the phylogenetic relationship and genetic diversity of Prunus. The reconstruction using the ITS region is to evaluate the evolutionary relationship of our species collected from North East region and to reflect on the relatedness of the taxa based on the ML and Bayesian method. The result of phylogenetic analyses based on ITS sequence clustered two major groups comprising of the Cerasus-Padus-Laurocerasus complex and the Prunus-Amygdalus complex.
Conclusion
The ITS sequences of the Prunus species from the present investigation would be helpful in the future study as it provides access to the sequences from the region of North East India as there is no phylogenetic study conducted till date.
Heavy rainfall events in the state of Acre, western Amazonia
Authors: Moreira José Genivaldo do Vale , de Almeida Maila Pereira , Silva Sonaira Souza da , Duarte Sergio Nascimento , Mendonça Fernando Campos 
Published: February 2023

Abstract

Currently, humanity is unable to prevent extreme hydrological events from occurring. However, the use of appropriate tools to estimate the probability associated with different hydrological variables, such as heavy rainfall, can help mitigate social and environmental damage. The objective of this study was to verify the hypothesis that the maximum rainfall records (over different intervals of duration) from the three main rainfall stations in the state of Acre fit the Gumbel distribution. Additionally, the periods of intense rainfall were evaluated based on the association between rainfall duration and return period. Using the maximum likelihood method, and once the baseline stationarity assumption established by the Mann-Kendall test was met, the relevant equations were fitted to the classical Gumbel model. Adherence to the model was assessed using the Anderson‒Darling test. The fitted distributions allowed the estimation of rainfall intensity for different intervals of duration and return periods for the cities of Cruzeiro do Sul, Tarauacá and Rio Branco. These results are relevant for the implementation of plans aimed at strengthening the water security in the state of Acre.
Induction of antitumor immunity by CCR2 transduced CD8+TILs in mouse lung carcinoma
Authors: Wan Yi , Fan TianYu , Wang Xin , Wang Bin , Zhang Bei , Zhang Li 
Published: February 2023

AbstractDespite recent therapeutic progress in lung carcinoma, the overall survival still remains poor. Therefore, exploration of novel therapeutic strategies remains a potential objective. Chemokine was first discovered as chemotactic factor during inflammation. The CCL2-CCR2 axis is one of the major chemokine signaling pathways, and has multiple functions in tumor microenvironment, such as tumor cell proliferation and invasiveness, angiogenesis, and recruitment of immune cells. In our previous study, CCR2 was found with the distinct expression modes from other cancer types such as breast cancer, pancreatic ductal adenocarcinoma, or prostate cancer: The expression was positive correlation with the prognosis and negative correlation with T and N classifications of lung carcinoma, and was higher in antitumor immune cells of CD8 + TILs, M1 macrophages, and active/resting CD4 + T memory cells compared other tumor-promoting immune cells. In the present study, we verified CCR2 expression in human and mouse lung carcinoma, and the antitumor functions were studied in mouse lung carcinoma isolated CD8 + TILs. We found that retroviral CCR2 transduction led to the activation in CD8 + TILs, improvement of its migration to the tumor microenvironment, promotion of the early apoptosis of tumor cells, and inhibition of the proliferation of tumor cells. All the data implied the antitumor function of CD8 + TILs with up-regulated CCR2 in lung carcinoma may be provide novel targeting therapeutic strategy in the near future.
Correlation between CBR and DCPT studies of different soils
Authors: ponduri Samatha chowdary , krosuri Siva Prasada rao , ponduri Siddharth chowdary 
Published: February 2023

Abstract
California bearing ratio test commonly referred as CBR test is an important test to be conducted for design of any pavement. However, since CBR can’t be easily determined in the field, the need for prediction of CBR from other soil support tests such as Dynamic cone penetrometer is a valuable alternative. In the present study dynamic cone penetrometer tests from the field were correlated with the CBR studies conducted in the laboratory. Unique models were developed for each classification of soil with good coefficient of determination (R2) and low standard error of estimate. The data thus combined gave a correlation between CBR and D that compare very well with studies from various other data.
Spontaneous pneumomediastinum in a COVID-19 patient after renal transplantation: a case report and literature review
Authors: Li Shaobo , Sikong Yinhe , Li Jianjun , Ding Meiling 
Published: February 2023

Abstract
Background: Spontaneous pneumomediastinum is a rare complication in COVID-19. Severe pneumonia complicated with pneumomediastinum after renal transplantation is rarely reported. Here we report a case of pneumomediastinum before invasive mechanical ventilation (IMV) in a COVID-19 patient with long-term immunosuppressive therapy after renal transplantation.
Case presentation: A 57-year-old man was admitted to our center with the main complaint of “fever and dyspnea for 5 days”. His past medical history was notable for renal transplantation and with long-term immunosuppressive and anti-rejection therapy. We made the diagnosis as COVID-19 pneumonia (severe type). We managed the patient with high flow nasal cannulae (HFNC), oral dexamethasone, broad-spectrum antibiotic, prophylactic anticoagulation, and anti-viral therapy with reduced dose of azvudine due to severe renal insufficiency. During hospitalization, the patient suffered from several times of aggravation of dyspnea. First bedside chest X-ray showed suspicious pneumomediastinum and subcutaneous emphysema, and the gas in the mediastinum gradually increased. The patient’s status deteriorated rapidly, we performed urgent trachea intubation and mechanical ventilation with low tidal volume lung-protective model and performed mediastinal decompression by suprasternal drainage. Despite our active rescue efforts, the patient still died of severe infection and multiple organ failure.
Conclusions: In conclusion, we are the first to report spontaneous pneumomediastinum in a renal transplant recipient with severe COVID-19 pneumonia. This case reminds us that pneumomediastinum is a severe complication and a poor prognostic factor of COVID-19 pneumonia, especially when it occurred without positive pressure ventilation and in immunocompromised patients.
A cytokine-responsive promoter is required for distal enhancer function mediating the hundreds-fold increase in milk protein gene expression during lactation
Authors: Hennighausen Lothar , Lee Hye Kyung , Liu Chengyu 
Published: February 2023

Abstract
During lactation, specialized cells in the mammary gland produce milk to nourish the young. Milk protein genes are controlled by distal enhancers activating expression several hundred-fold during lactation. However, the role of promoter elements is not understood. We addressed this issue using the Csn2 gene, which accounts for 10% of mRNA in mammary tissue. We identified STAT5 and other mammary transcription factors binding to three distal candidate enhancers and a cytokine-response promoter element. While deletion of the enhancers or the introduction of an inactivating mutation in a single promoter element had a marginable effect, their combined loss led to a 99.99% reduction of Csn2 expression. Our findings reveal the essential role of a promoter element in the exceptional activation of a milk protein gene and highlight the importance of analyzing regulatory elements in their native genomic context to fully understand the multifaceted functions of enhancer clusters and promoters.
A case of brucellosis spondylitis complicated by neurobrucellosis-a Case Report
Authors: Zhu Shu-lai , Pan Xin-xin , Liu Ying , Peng Yu-hua 
Published: February 2023

Abstract
Introduction: We report a case of neurobrucellosis following brucellosis spondylitis. To date ,this is the first description of a patient suffering neurobrucellosis following brucellosis spondylitis.
Case Presentation: The patient was an elderly men with a history of feeding sheep and cows. The common symptoms included cervicoldynia and progressive paralysis of limbs. According to the results of blood bacterial culture, brucella agglutination test, CSF culture, tissue culture and Next-generation sequencing, the patient was considered meningitis following brucellosis spondylitis. According to the progressive weakness of the limbs, the patient received discectomy and anti-brucella therapy, the clinical symptoms gradually improved. But remained with mild paralysis of limbs although by 1-month rehabilitative training.
Conclusion: When central nervous system presented with patient of brucellosis spondylitis, we should be aware of neurobrucellosis.
Endophenotype Network-based Approach reveals the Pharmacological Mechanism of Osthole against D-Galactose Induced Cognitive Disorder in Rats
Authors: Fu Xiaomei , Wang Xue , Lai Yiyi , Cai Chuipu , Liao Yanfang , Dai Zhao , Xu Huilin , Fang Shuhuan , Fang Jiansong 
Published: February 2023

AbstractBackground:Osthole, a natural coumarin, found in many medicinal plants. Previous studies have shown its neuroprotective effects, whereas the effect and fundamental mechanism of Osthole for alleviating AD-associated dysmnesia is still not fully clear.Purpose:This study aimed to examine the neuroprotection of Osthole against cognitive impairment in the D-galactose-induced rats and its pharmacological mechanism.Method:The rat was constructed by subcutaneous injection of D-galactose at a dose of 150 mg/kg/day for 56 days as a model. The effect of Osthole on cognitive impairment was evaluated by behavior and biochemical analysis. Subsequently, a combination ofin silicoprediction and experimental validation was performed todetermine the underlying mechanisms of Osthole against Alzheimer's disease, while to verify the network-based predictions, western blot, Nissl staining, and immunofluorescence were applied.Result:Osthole could improve memory dysfunction induced by D-galactose in Sprague Dawley male rat. Endophenotype-based network approach highlight several AD-related pathological processes that may be regulated by Osthole, including neuronal apoptosis, neuroinflammationand endoplasmic reticulum stress. Among them, the proapoptotic markers (Bax), antiapoptotic protein (Bcl-2), moreover, the microgliosis (Iba-1), Astrocytosis (GFAP), and inflammatory cytokines (TNF-α1), levels of ER stress-associated proteins (BIP, p-PERK/PERK, Caspase12, CHOP and XBP1s) were evaluated in both hippocampus and cortex. And the results indicated that Osthole significantly ameliorated neuronal apoptosis, neuroinflammation and ER stress in D-galactose induced rats.Conclusion:This study explored the pharmacological mechanism of Osthole against D-galactose induced memory impairment and identified Osthole as a potential anti-AD drug candidate targeting multiple signaling pathways by endophenotype network-based.
Universal Shape Replication Via Self-Assembly With Signal-Passing Tiles
Authors: Alseth Andrew , Hader Daniel , Patitz Matthew J. 
Published: February 2023

Abstract

In this paper, we investigate shape-assembling power of a tile-based model of self-assembly called the Signal-Passing Tile Assembly Model (STAM). In this model, the glues that bind tiles together can be turned on and off by the binding actions of other glues via "signals". Specifically, the problem we investigate is "shape replication" wherein, given a set of input assemblies of arbitrary shape, a system must construct an arbitrary number of assemblies with the same shapes and, with the exception of size-bounded junk assemblies that result from the process, no others. We provide the first fully universal shape replication result, namely a single tile set capable of performing shape replication on arbitrary sets of any 3-dimensional shapes without requiring any scaling or pre-encoded information in the input assemblies. Our result requires the input assemblies to be composed of signal-passing tiles whose glues can be deactivated to allow deconstruction of those assemblies, which we also prove is necessary by showing that there are shapes whose geometry cannot be replicated without deconstruction. Additionally, we modularize our construction to create systems capable of creating binary encodings of arbitrary shapes, and building arbitrary shapes from their encodings. Because the STAM is capable of universal computation, this then allows for arbitrary programs to be run within an STAM system, using the shape encodings as input, so that any computable transformation can be performed on the shapes. This is the full version, containing all construction and proof details, of a previously published extended abstract version that had most details omitted.
Efficient Removal of Methylene Blue by Micro-/Mesoporous Heteroatom-doped Biochar Derived from Waste Bovine Horn
Authors: Peng Qifan , Du Zhen , Ma Chaoqiang , Guan Yuepeng , Feng Zhenxing , Huang Yaqin 
Published: February 2023

Abstract
Efficient removal of dyes from wastewater has become an urgent issue due to the rapid industrial development and increasing health concern. Here, micro-/mesoporous heteroatom-doped biochars (BHBs) were prepared from waste bovine horn by controlled pyrolysis. The BHB that had undergone a pre-oxidation reaction before pyrolysis has honeycomb-like porous structure with high specific surface area of 2447.41 m2 g− 1 and the pore size of lower than 5 nm. The tunable porous structure of the biochar could shorten the travel distance of dye molecules from external surface of biochar to inner surface of pores. And the heteroatoms could enhance the interactions, including electrostatic interaction and hydrogen bonding interaction between carbon surface and methylene blue molecules. For adsorption of methylene blue, the BHB exhibited high adsorption capacity of 1720.92 mg g− 1 at 30 ℃ and could reach a removal efficiency of near 100% in 0.5 h. The excellent adsorption performance of BHB for dye makes it a potential adsorbent for wastewater purification.
Factors associated with risk sexual behaviors among sexually active college students in Sichuan, China: a cross- sectional survey
Authors: Dai Yingxue , Li Yajie , Zhou Dinglun , Zhang Jianxin 
Published: February 2023

Abstract
Background
Males account for a significant share of new infections among young people in China. This study aimed to identify the factors associated with risk sexual behaviours, and provide evidence of leverage for effective interventions among male college students.
Methods
The cross-sectional study was conducted from December 2020 to December 2021 in 16 colleges, located in Sichuan Province, one of the high-risk areas in China. Overall 1640 male college students who reported sexually experienced were analyzed in this study. Multivariable logistic regression analysis was applied to determine factors associated with risk sexual behaviours.
Results
The average age of included male students was 19.95 ± 1.56. Of them, 27.74% initiated sexual behavior early, and 16.52% did not use condoms at the latest sexual intercourse. Students who were older (age 20-21, AOR=0.43, 95%CI: 0.33-0.55; age≥22, AOR=0.13, 95%CI: 0.08-0.21) (versus age ≤ 19) were less likely to have early sexual debut. The likelihood of engaging in condomless sex at the latest sexual intercourse was lower among male college students who had a senior high school educated mother (vs junior high school and below, AOR=0.47, 95%CI: 0.28-0.80), were knowledgeable about sex and reproductive health (vs unknowledgeable, AOR=0.48, 95%CI: 0.27-0.85), and held a higher level of condom use efficacy confident vs unconfident, AOR=0.50, 95%CI: 0.35-0.71; very confident vs unconfident, AOR=0.26, 95%CI: 0.16-0.42). The odds of condomless sex at the latest sexual intercourse increased among participants reporting perceived risk of HIV infection (vs no, AOR=1.96, 95%CI: 1.27-3.03) and early sexual debut (vs no, AOR=1.61, 95%CI: 1.12-2.32).
Conclusion
Comprehensive sex education curricula might be an effective approach by emphasizing fundamental sex and reproductive information and empowering students with assertiveness and negotiation skills with regard to condom use during or before college.
An optimized CT-dense agent perfusion and micro-CT imaging protocol for chick embryo developmental stages
Authors: Naija Azza , Mutlu Onur , Khan Talha , Seers Thomas Daniel , Yalcin Huseyin C 
Published: February 2023

Abstract

Compared to classical techniques of morphological analysis, micro-CT (µ-CT) has become an effective approach allowing rapid screening of morphological changes. In the present work, we aimed to provide an optimized µ-CT dense agent perfusion protocol and µ-CT guidelines for different stages of chick embryo cardiogenesis. Our study was conducted over a period of 10 embryonic days (Hamburger-Hamilton HH36) in chick embryo hearts. During the perfusion of the µ-CT dense agent at different developmental stages (HH19, HH24, HH27, HH29, HH31, HH34, HH35, and HH36), we demonstrated that durations and volumes of the injected contrast agent gradually increased with the heart developmental stages contrary to the flow rate that was unchanged during the whole experiment. Analysis of the CT imaging confirmed the efficiency of the optimized parameters of the heart perfusion.
Synonymous Codon Pattern of Cauliflower Mosaic Virus in phylogeny and evolution
Authors: Pouresmaeil Mahin , Pazhouhandeh Maghsoud , Shirzad Akbar , Khosroushahi Ahmad Yari 
Published: February 2023

Abstract

Cauliflower Mosaic Virus (CaMV) is a plant Pararetrovirus with a double-stranded DNA genome distributed worldwide. This study analyzed migration, evolution, and synonymous codon pattern of CaMV and the factors that shape it. We extracted genomic sequences of 121 isolates of CaMV, which were reported from various regions-hosts, from the NCBI database. The evolution of viruses has been widely studied by analyzing their nucleotides and coding regions/codons using different methods. Analysis of the CaMV phylogenetic tree shows that it divides most of the sequences into two main groups: Group I includes Irananin, Japanese, and American-European subgroups, and Group II includes Grecian, Turkish, and Iranian subgroups. Analysis of effective codon count, and relative codon deoptimization index, showed that natural selection is a major driving force in CaMV. Furthermore, Relative synonymous codon usage (RSCU) and neutrality analyses show that CaMV prefers A-ending codons and that one codon, namely GGA, was overrepresented. Analysis of dinucleotide composition demonstrates that nucleotide A was the most abundant in the CaMV coding sequences, and that the most frequent nucleotide at the third position of the codon was A3S. In CaMV, host adaptation was highest for Brassica oleracea and lowest for Raphanus sativus. Therefore the CaMV codon pattern is mostly shaped by the need to escape antiviral responses associated with host dinucleotides and translational efficiency. These values indicate that the study provides useful information on the codon usage analysis of CaMV and can be used to understand host adaptation to the virus environment and its evolution. This is the first study on codon usage bias of CaMV in the world.
Analysis of Prognostic Value, Immune Escape Significance and Ferroptosis Correlation of Ubiquitination-related Genes UBTD1 in Colorectal Carcinogenesis
Authors: Jin Yuzhao , Chen Qianping , Tang Bufu , Mao Wei , Wang Lu , Lan Huiyin , Bai Minghua , Zhu Ji , jiang jin 
Published: February 2023

Abstract
Immunotherapy based on immune checkpoint inhibitors is currently a hot topic of investigation in the therapy of colorectal cancer (CRC). The most reliable predictors of immune effectiveness are, at present, microsatellite instability (MSI) and mismatch repair gene status (MMR).In contrast, other immune efficacy predictors remain desirable to facilitate individualized immunotherapy for CRC patients. Ubiquitination and its associated ubiquitin-binding enzymes and ligases regulate the tumor microenvironment and antitumor immunity to mediate tumor pathogenesis and progression. Here, we examined the molecular characteristics and immunomodulatory effects of ubiquitination-associated genes mediating the prognosis of CRC cancer. UBTD1 was identified as a significant prognostic, predictive gene for CRC, involved in regulating the immune checkpoint levels and immune cell function of CRC patients. Briefly, high expression of UBTD1 tended to enhance the presence of immune checkpoints to induce immune escape and inhibit the onset of ferroptosis. Our study demonstrated that UBTD1 was a prognostic marker for CRC in the regulation of ubiquitination and the tumor immune microenvironment and may serve as a predictor of immune efficacy and a modulator of resistance to immune checkpoint suppressive therapies.