bluuebunny/crossref_metadata_2025_split huggingface dataset:
The atherogenic index of plasma (AIP) is a predictor for the severity of coronary artery disease
Authors: Li Ya , Feng Yujia , Li Shu , Ma Yulin , Lin Jiesheng , Wan Jing , Zhao Min 
Published: June 2023

ObjectiveDyslipidemia is a key risk factor for coronary artery disease (CAD). This study aimed to investigate the correlation between the atherogenic index of plasma (AIP) and the severity of CAD.Methods2,491 patients were enrolled in this study and analyzed retrospectively, including 665 non-CAD patients as the control group and 1,826 CAD patients. The CAD patients were classified into three subgroups according to tertiles of SYNTAX score (SS). Non-high-density lipoprotein cholesterol (Non-HDL-C) was defined as serum total cholesterol (TC) minus serum high-density lipoprotein cholesterol (Non-HDL-C), atherogenic index (AI) was defined as the ratio of non-HDL-C to HDL-C; AIP was defined as the logarithm of the ratio of the concentration of triglyceride (TG) to HDL-C; lipoprotein combine index (LCI) was defined as the ratio of TC∗TG∗ low-density lipoprotein cholesterol (LDL)to HDL-C; Castelli Risk Index I (CRI I) was defined as the ratio of TC to HDL-C; Castelli Risk Index II (CRI II) was defined as the ratio of LDL-C to HDL-C.ResultsThe levels of AIP (P &lt; 0.001), AI (P &lt; 0.001), and LCI (P = 0.013) were higher in the CAD group compared with the non-CAD group. The Spearman correlation analysis showed that AIP (r = 0.075, P &lt; 0.001), AI (r = 0.132, P &lt; 0.001), and LCI (r = 0.072, P = 0.001) were positively correlated with SS. The multivariate logistic regression model showed CRI I (OR: 1.11, 95% CI: 1.03–1.19, P = 0.005), CRI II (OR: 1.26, 95% CI: 1.15–1.39, P &lt; 0.001), AI (OR: 1.28, 95% CI: 1.17–1.40, P &lt; 0.001), AIP (OR: 2.06, 95% CI: 1.38–3.07, P &lt; 0.001), and LCI (OR: 1.01, 95% CI: 1.01–1.02, P &lt; 0.001) were independent predictors of severity of CAD After adjusting various confounders.ConclusionCRI I, CRI II, AIP, AI, and LCI were independent predictors of the severity of CAD, which could be used as a biomarker for the evaluation of the severity of CAD.
The pattern of late gadolinium enhancement by cardiac MRI in fulminant myocarditis and its prognostic implication: a two-year follow-up study
Authors: Jiang Luying , Zuo Houjuan , Liu Jingbo , Wang Jianyu , Zhang Kaiyue , Zhang Chunran , Peng Xiangyang , Liu Yujian , Wang Daowen , Li Haojie , Wang Hong 
Published: June 2023

BackgroundMyocardial fibrosis, as quantified by late gadolinium enhancement (LGE) in cardiac magnetic resonance (CMR), provides valuable prognostic information for patients with myocarditis. However, due to the low incidence rate of fulminant myocarditis (FM) and accordingly small sample size, the knowledge about the role of LGE to patients with FM is limited.Methods and resultsA total of 44 adults with viral-FM receiving the Chinese treating regimen were included in this retrospective study. They were divided into the low LGE group and the high LGE group according to the ratio of LGE to left ventricular mass (LGE mass%). CMR exams and LGE were performed after hemodynamic assistance at discharge in all patients with FM. Routine echocardiography parameters and global longitudinal strain (GLS) at discharge and at 2-year follow-up were obtained and then compared. Both left ventricular ejection fraction (LVEF) and GLS showed no significant difference in both groups at discharge, whereas significant differences were observed at 2-year follow-up between two groups. Moreover, there were significant improvements of LVEF and GLS in the low LGE group, but not in the high LGE group during the 2-year period. Furthermore, LGE mass% was negatively correlated with GLS and LVEF.ConclusionsThere were two distinct forms of LGE presentation in patients with FM. Moreover, the cardiac function of patients with low LGE was significantly better than those with high LGE at 2-year follow-up. LGE mass% at discharge provided significant prognosis information about cardiac function of patients with FM.
IFABP levels predict visceral malperfusion in the first hours after open thoracoabdominal aortic repair
Authors: Doukas Panagiotis , Bassett Cathryn , Krabbe Hanif , Frankort Jelle , Jacobs Michael J. , Elfeky Moustafa , Gombert Alexander 
Published: June 2023

IntroductionIntestinal ischemia after open thoracoabdominal aortic repairs, is a rare but devastating complication, associated with high mortality. Notoriously challenging to diagnose, visceral malperfusion necessitates immediate surgical attention. Intestinal fatty acid-binding protein (IFABP) has been proposed as a biomarker for the diagnosis of intestinal wall damage. In this prospectively conducted, observational study we evaluated the diagnostic capacity of IFABP levels in patients' serum and their correlation with visceral malperfusion.Methods23 patients undergoing open thoracoabdominal aortic repairs were included in this study and 8 of them were diagnosed postoperatively with visceral malperfusion—defined as a partial or complete thrombotic occlusion of the superior mesenteric artery and/or the coeliac trunk. IFABP levels and laboratory parameters often associated with intestinal ischemia (leucocytes, CRP, PCT and lactate) were measured at baseline, directly postoperatively, and at 12, 24 and 48 h after surgery. Postoperative visceral malperfusion—as revealed in CT angiography—was assessed and the predictive ability of IFABP levels to detect visceral malperfusion was evaluated with receiver-operator curve analysis.ResultsPatients with visceral malperfusion had a relevant risk for a fatal outcome (p = .001). IFABP levels were significantly elevated directly postoperatively and at 12 h after surgery in cases of visceral malperfusion. High IFABP concentrations in serum detected visceral malperfusion accurately during the first 12 h after surgery, with the maximum diagnostic ability achieved immediately after surgery (AUC 1, Sensitivity 100%, Specificity 100%, p &lt; .001).ConclusionWe conclude, that IFABP measurements during the first postoperative hours after open thoracoabdominal aortic surgery can be a valuable tool for reliable and timely detection of visceral malperfusion.
Chaihu-Longgu-Muli decoction improves sleep disorders by restoring orexin-A function in CKD mice
Authors: Cao Xin-li , Peng Xue-mei , Li Gong-bo , Ding Wei-sen , Wang Kai-zhen , Wang Xiao-lei , Xiong Yan-ying , Xiong Wei-jian , Li Fan , Song Min 
Published: June 2023

IntroductionChaihu-Longgu-Muli decoction (CLMD) is a well-used ancient formula originally recorded in the “Treatise on Febrile Diseases” written by the founding theorist of Traditional Chinese Medicine, Doctor Zhang Zhongjing. While it has been used extensively as a therapeutic treatment for neuropsychiatric disorders, such as insomnia, anxiety and dementia, its mechanisms remain unclear.MethodsIn order to analyze the therapeutic mechanism of CLMD in chronic renal failure and insomnia, An adenine diet-induced chronic kidney disease (CKD) model was established in mice, Furthermore, we analyzed the impact of CLMD on sleep behavior and cognitive function in CKD mice, as well as the production of insomnia related regulatory proteins and inflammatory factors.ResultsCLMD significantly improved circadian rhythm and sleep disturbance in CKD mice. The insomnia related regulatory proteins, Orexin, Orexin R1, and Orexin R2 in the hypothalamus of CKD mice decreased significantly, while Orexin and its receptors increased remarkably after CLMD intervention. Following administration of CLMD, reduced neuron loss and improved learning as well as memory ability were observed in CKD mice. And CLMD intervention effectively improved the chronic inflflammatory state of CKD mice.DiscussionOur results showed that CLMD could improve sleep and cognitive levels in CKD mice. The mechanism may be related to the up-regulation of Orexin-A and increased phosphorylation level of CaMKK2/AMPK, which further inhibits NF-κB downstream signaling pathways, thereby improving the disordered inflammatory state in the central and peripheral system. However, More research is required to confirm the clinical significance of the study.
Agricultural socialized services to stimulate the green production behavior of smallholder farmers: the case of fertilization of rice production in south China
Authors: Shi Fang , Cai Baozhong , Meseretchanie Abate , Geremew Betelhemabraham , Huang Yuangji 
Published: June 2023

Introduction: This study investigates the impact of Agricultural Socialized Services (ASSs) on the green production behavior (GPB) of smallholder farmers in the main rice production region of southern China. The research aims to address the gap in understanding the relationship between ASSs and the adoption of sustainable agricultural practices by smallholder farmers.Methodology: Data was collected from 741 smallholder farmers in Hunan, Jiangxi, and Zhejiang provinces using a structured questionnaire. A probit model was employed to examine the relationship between ASSs and smallholder farmers’ GPB.Results and Discussion: The study revealed that ASSs have a significant and positive impact on smallholder farmers’ GPB. Farmers who received ASSs tended to adopt more sustainable agricultural practices such as using organic fertilizer and soil-tested formula fertilizer. The findings of this study have important implications for policymakers. The results suggest that policymakers should prioritize the development of services to agricultural producers and strengthen Agricultural Service Systems. This can be achieved through optimizing the public administration service system, establishing joint service organizations, and creating a good financial and legal service environment. By doing so, policymakers can promote the adoption of sustainable agricultural practices and the overall development of the agricultural sector.
Platelet-derived microparticles provoke chronic lymphocytic leukemia malignancy through metabolic reprogramming
Authors: Gharib Ehsan , Veilleux Vanessa , Boudreau Luc H. , Pichaud Nicolas , Robichaud Gilles A. 
Published: June 2023

BackgroundIt is well established that inflammation and platelets promote multiple processes of cancer malignancy. Recently, platelets have received attention for their role in carcinogenesis through the production of microvesicles or platelet-derived microparticles (PMPs), which transfer their biological content to cancer cells. We have previously characterized a new subpopulation of these microparticles (termed mito-microparticles), which package functional mitochondria. The potential of mitochondria transfer to cancer cells is particularly impactful as many aspects of mitochondrial biology (i.e., cell growth, apoptosis inhibition, and drug resistance) coincide with cancer hallmarks and disease progression. These metabolic aspects are particularly notable in chronic lymphocytic leukemia (CLL), which is characterized by a relentless accumulation of proliferating, immunologically dysfunctional, mature B-lymphocytes that fail to undergo apoptosis. The present study aimed to investigate the role of PMPs on CLL metabolic plasticity leading to cancer cell phenotypic changes.MethodsCLL cell lines were co-incubated with different concentrations of human PMPs, and their impact on cell proliferation, mitochondrial DNA copy number, OCR level, ATP production, and ROS content was evaluated. Essential genes involved in metabolic-reprogramming were identified using the bioinformatics tools, examined between patients with early and advanced CLL stages, and then validated in PMP-recipient CLLs. Finally, the impact of the induced metabolic reprogramming on CLLs’ growth, survival, mobility, and invasiveness was tested against anti-cancer drugs Cytarabine, Venetoclax, and Plumbagin.ResultsThe data demonstrated the potency of PMPs in inducing tumoral growth and invasiveness in CLLs through mitochondrial internalization and OXPHOS stimulation which was in line with metabolic shift reported in CLL patients from early to advanced stages. This metabolic rewiring also improved CLL cells' resistance to Cytarabine, Venetoclax, and Plumbagin chemo drugs.ConclusionAltogether, these findings depict a new platelet-mediated pathway of cancer pathogenesis. We also highlight the impact of PMPs in CLL metabolic reprogramming and disease progression.
Marine biodiversity discovery: the metrics of new species descriptions
Authors: Bouchet Philippe , Decock Wim , Lonneville Britt , Vanhoorne Bart , Vandepitte Leen 
Published: June 2023

Based on the World Register of Marine Species (WoRMS), there are currently c. 242,000 known valid marine species living in the world's oceans and marine biota continue to be discovered and named steadily at a current average of 2,332 new species per year. The “average” newly described marine species is a benthic crustacean, annelid, or mollusc between 2 and 10 mm in size, living in the tropics at depths of 0–60 m, and represented in the description by 7–19 specimens. It is described after a shelf life of 13.5 years in an article with two to three authors in a journal with an IF &lt;1, published by an academic institution or society or a small commercial publisher. It is highly likely that the description is not accompanied by molecular data and that its authors do not work in an institution in a region of the world where the new species comes from. At the current pace of discovery and characterization, it will take several hundred years to describe the remaining 1–2 million unknown marine species. With increased facilitation of access to literature, marine taxonomy will increasingly rely on retired professionals and citizen scientists. The barriers to new marine species descriptions are in part technological (access to habitats that are difficult to sample) and educational (training to generate and use molecular barcodes), but mostly institutional (funding of taxonomic work) and regulatory (restrictions imposed by access and benefit sharing legislation).
Systematic review of SLC4A11, ZEB1, LOXHD1, and AGBL1 variants in the development of Fuchs’ endothelial corneal dystrophy
Authors: Tsedilina Tatiana Romanovna , Sharova Elena , Iakovets Valeriia , Skorodumova Liubov Olegovna 
Published: June 2023

IntroductionThe pathogenic role of variants in TCF4 and COL8A2 in causing Fuchs’ endothelial corneal dystrophy (FECD) is not controversial and has been confirmed by numerous studies. The causal role of other genes, SLC4A11, ZEB1, LOXHD1, and AGBL1, which have been reported to be associated with FECD, is more complicated and less obvious. We performed a systematic review of the variants in the above-mentioned genes in FECD cases, taking into account the currently available population frequency information, transcriptomic data, and the results of functional studies to assess their pathogenicity.MethodsSearch for articles published in 2005–2022 was performed manually between July 2022 and February 2023. We searched for original research articles in peer-reviewed journals, written in English. Variants in the genes of interest identified in patients with FECD were extracted for the analysis. We classified each presented variant by pathogenicity status according to the ACMG criteria implemented in the Varsome tool. Diagnosis, segregation data, presence of affected relatives, functional analysis results, and gene expression in the corneal endothelium were taken into account. Data on the expression of genes of interest in the corneal endothelium were extracted from articles in which transcriptome analysis was performed. The identification of at least one variant in a gene classified as pathogenic or significantly associated with FECD was required to confirm the causal role of the gene in FECD.ResultsThe analysis included 34 articles with 102 unique ZEB1 variants, 20 articles with 64 SLC4A11 variants, six articles with 26 LOXHD1 variants, and five articles with four AGBL1 variants. Pathogenic status was confirmed for seven SLC4A11 variants found in FECD. No variants in ZEB1, LOXHD1, and AGBL1 genes were classified as pathogenic for FECD. According to the transcriptome data, AGBL1 and LOXHD1 were not expressed in the corneal endothelium. Functional evidence for the association of LOXHD1, and AGBL1 with FECD was conflicting.ConclusionOur analysis confirmed the causal role of SLC4A11 variants in the development of FECD. The causal role of ZEB1, LOXHD1, and AGBL1 variants in FECD has not been confirmed. Further evidence from familial cases and functional analysis is needed to confirm their causal roles in FECD.
Atopic dermatitis and chronic kidney disease: a bidirectional Mendelian randomization study
Authors: Zhang Han , Yuan Shuai , Li Yong , Li Doudou , Yu Zengli , Hu Lidan , Li Xue , Wang Yuming , Larsson Susanna C. 
Published: June 2023

BackgroundA bidirectional association between atopic dermatitis and chronic kidney disease (CKD) has been revealed in observational studies, whereas the causality of this association was unclear. We conducted a Mendelian randomization study to determine the bidirectional causal association between atopic dermatitis and CKD.MethodsIndependent genetic instruments associated with atopic dermatitis and CKD at the genome-wide significance level were chosen from corresponding meta-analyses of genome-wide association studies. Summary-level data for atopic dermatitis were obtained from the EAGLE Eczema consortium (30,047 cases and 40,835 controls) and FinnGen consortium (7,024 cases and 198,740 controls). Summary-level data for CKD were derived from CKDGen consortium (64,164 cases and 625,219 controls) and FinnGen consortium (3,902 cases and 212,841 controls). The inverse-variance weighted method was used in the main analysis and supplemented with three sensitivity analyses.ResultsGenetic predisposition to atopic dermatitis was associated with an increased risk of CKD. For a one-unit increase in the prevalence of atopic dermatitis, the odds ratio of CKD was 1.07 (95% confidence interval: 1.01–1.12). In the reverse Mendelian randomization analysis, the odds ratio of atopic dermatitis was 1.14 (95% confidence interval: 1.03–1.26) for a one-unit increase in the prevalence of CKD. The associations persisted in sensitivity analyses and no pleiotropy was detected.ConclusionThis Mendelian randomization study suggests a bidirectional positive association between atopic dermatitis and CKD.
Qi-fu-yin attenuated cognitive disorders in 5xFAD mice of Alzheimer's disease animal model by regulating immunity
Authors: Yang Xiuzhao , Ye Tianyuan , He Yun , Wen Lei , Cheng Xiaorui 
Published: June 2023

IntroductionCognitive impairment is the main symptom of Alzheimer's disease (AD). Accumulating evidence implicate that immunity plays an important role in AD. Here, we investigated the effect of Qi-fu-yin (QFY) on cognitive impairment and cytokine secretion of 5xFAD mice.MethodsWe used 2.5-month-old 5xFAD transgenic mice for behavioral tests to observe the changes in cognitive function after QFY treatment. After the behavioral experiment, the whole brain, cortex and plasma of each mouse were collected for soluble Aβ analysis, immunohistochemical experiment and cytokine analysis.ResultsHere we found that the treatment of QFY ameliorated the ability of object recognition, passive avoidance responses and the ability of spatial learning and memory in 5xFAD mice. The deposits of β1 − 42 and Aβ1 − 40 were alleviated and the ration of Aβ1 − 42/Aβ1 − 40 was decrease in the plasma and brain of 5xFAD mice administrated with QFY. The administration of QFY promoted the secretion of anti-inflammatory cytokines, IL-5, IL-10 and G-CSF, and reduced the content of proinflammatory cytokines IFN-γ in plasma of 5xFAD mice. Notably, we found that the treatment of QFY decreased the concentration of CCL11 in the brain and plasma of 5xFAD mice.ConclusionThis suggested that QFY improved cognition and reduced Aβ deposits in 5xFAD mice by regulating abnormal immunity in 5xFAD mice. QFY may be as a potential therapeutic agent for AD.
Plasma lipidomic profiling of thiopurine-induced leukopenia after NUDT15 genotype-guided dosing in Chinese IBD patients
Authors: Li Pan , Chao Kang , Hu Zhanhua , Qin Lulu , Yang Ting , Mao Jing , Zhu Xia , Hu Pinjin , Wang Xueding , Gao Xiang , Huang Min 
Published: June 2023

IntroductionThiopurines, azathiopurine (AZA) and mercaptopurine (6-MP) have been regularly used in the treatment of inflammatory bowel disease (IBD). Despite optimized dosage adjustment based on the NUDT15 genotypes, some patients still discontinue or change treatment regimens due to thiopurine-induced leukopenia.MethodsWe proposed a prospective observational study of lipidomics to reveal the lipids perturbations associated with thiopurine-induced leukopenia. One hundred and twenty-seven IBD participants treated with thiopurine were enrolled, twenty-seven of which have developed thiopurine-induced leucopenia. Plasma lipid profiles were measured using Ultra-High-Performance Liquid Chromatography-Tandem Q-Exactive. Lipidomic alterations were validated with an independent validation cohort (leukopenia n = 26, non-leukopenia n = 74).ResultsUsing univariate and multivariate analysis, there were 16 lipid species from four lipid classes, triglyceride (n = 11), sphingomyelin (n = 1), phosphatidylcholine (n = 1) and lactosylceramide (n = 3) identified. Based on machine learning feature reduction and variable screening strategies, the random forest algorithm established by six lipids showed an excellent performance to distinguish the leukopenia group from the normal group, with a model accuracy of 95.28% (discovery cohort), 79.00% (validation cohort) and an area under the receiver operating characteristic (ROC) curve (ROC-AUC) of 0.9989 (discovery cohort), 0.8098 (validation cohort).DiscussionOur novel findings suggested that lipidomic provided unique insights into formulating individualized medication strategies for thiopurines in IBD patients.