bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA methylation and lipid metabolism in maize PCD DNA methylation and lipid metabolism are involved in maize aleurone PCD as revealed by transcriptome analysis
Authors: Wu Yequn , Hou Jiaqi , Ren Ruifei , Chen Zhenfei , Yue Mengxia , Li Le , Hou Haoli , Zheng Xueke , Li Lijia 
Published: August 2023

Abstract
Background
The aleurone layer is an integral part of many plant seeds, and during seed germination, aleurone cells undergo PCD, which is promoted by GA from the embryo. However, the numerous components of the GA signaling pathway that mediate PCD of the aleurone proteins remain to be identified. Few genes and transcriptomes have been studied thus far in aleurone layers to improve our understanding of how PCD occurs and how the regulatory mechanism functions during PCD. Our previous studies have shown that histone deacetylase (HDAC) is required in GA-induced PCD of aleurone layer. To further explore the molecular mechanisms by which epigenetic modifications regulate aleurone PCD, we performed a global comparative transcriptome analysis of embryoless aleurones treated with GA or histone acetylase (HAT) inhibitors.
Results
In this study, 7,919 differentially expressed genes (DEGs) had been analyzed, 2,554 DEGs of which were found to be common under two treatments. These DEGs were involved in various biological processes, including DNA methylation, lipid metabolism and ROS signaling. Further investigations revealed that inhibition of DNA methyltransferases prevented aleurone PCD, suggesting that active DNA methylation plays a role in regulating aleurone PCD. GA or HAT inhibitor induced lipoxygenase gene expression, leading to lipid degradation, but this process was not affected by DNA methylation. However, DNA methylation inhibitor could regulate ROS-related gene expression and inhibit GA-induced production of hydrogen peroxide (H2O2).
Conclusion
The linking of lipoxygenase, DNA methylation, and H2O2 may indicate that GA-induced higher HDAC activity in aleurones caused breakdown of lipids via regulating lipoxygenase gene expression, and increased DNA methylation positively mediated H2O2 production; thus, DNA methylation and lipid metabolism pathways may represent an important and complex signaling network in maize aleurone PCD.
Ionic Liquid Treated Bacterial Cellulose Sheets as Prospective Biodegradable Implant Materials
Authors: Munir Muniba , Nosheen Sadaf , Muhammad Nawshad , Uroos Maliha , Mustafa Waleed , Khan Rawaiz , Wang Rong , Sharif Faiza 
Published: August 2023

Abstract
The purpose of this research was to create BC membranes / sheets which can be degraded by the enzymes in body fluids on implantation for soft and hard tissue regeneration. Bacterial Cellulose has been explored for its use in hard and soft tissue regeneration such as bone, dental, wound, hernia, dura mater, skin, nerve, cornea, and blood vessels. The limiting factor in the use of BC as biomedical implant material is that it is practically non-biodegradable in vitro and in vivo. However, reactive hydroxyl groups on BC allow a variety of chemical modifications which can be beneficial for the development of smart degradable biomedical materials. The use of Ionic Liquids (ILs) is the greener and non-toxic alternative to the chemical treatment for the degradation of BC. The ILs affect the degradability of BC by interacting with the functional groups and decreasing its crystallinity.
Two non-toxic and biocompatible ILs i.e Pyridinium hydrogen sulfate (Py-HSO4) and 1-butyl-3-methyl imidazolium hydrogen sulfate (BMIM-HSO4) were used in the current study. The biodegradation of BC using these ILs has not been studied previously for biomedical implants. The characterizations of the IL treated BC were done using XRD, FTIR analysis, SEM, contact angle studies, degradation assay, drug delivery, and in vitro biocompatibility. SEM results suggest a clear change in the morphology of the BC nano fibers after treatment with ionic liquids. Furthermore, significant degradation was observed over 28 days where BC (Py-HSO4) degraded by 36% and BC (BMIM-HSO4) treated had degraded by 56%. Additionally, the IL treated BC could carry antibacterial drugs and showed potential for their sustained release. The modified membranes supported cell attachment and proliferation and were non-toxic and highly biocompatible. These results suggest that BC pellicles / sheets treated with ILs can be used as a degradable implant material for tissue engineering, regeneration, and drug delivery for various regenerative biomedical applications.
Reflections on the optimization of energy consumption of underground mine cars based on brake energy recovery
Authors: Hao Jian , Liu PengCheng , Hu Hui , Luan XueKun 
Published: August 2023

Abstract
Aiming at the problem that underground mine cars need to brake frequently in the mine and cause a large amount of energy waste, we analyzed the working characteristics of underground mine cars and put forward the idea of energy consumption optimization based on braking energy recovery; we designed a braking energy recovery device suitable for underground mine cars; we calculated the comparison of energy consumption before and after installing the energy recovery system in mine car locomotives through mathematical modeling, and estimated the energy recovery benefit in the operating conditions of the underground mines. The results show that in the operation process, the energy recovery system can be used to recover energy from the brakes. The results show that the braking energy recovery system can successfully recover more than 50% of the braking energy during operation, increase the range of the mine car motorcar by about 20%, significantly improve the energy utilization efficiency of the coal mine tram, and reduce the system energy consumption and CO2 emissions.
Pharmacokinetics of Ethanamizuril Dispersed by Different Solvents In Chickens After Oral Administration
Authors: Wang Xiaoyang , Wang Chunmei , Zhang Zhihao , Zhang Keyu , Xue Feiqun , Zhang Lifang , Fei Chenzhong , Hang Taijun 
Published: August 2023

Abstract
In this study Ethanamizuril in different dispersants were administrated orally to target animal, chickens for pharmacokinetic study. An ultra-performance liquid chromatography method was established for sample detection and the extraction method was validated. Internal standard was used to calculate the drug concentration in chicken plasma. The results showed that the peak time of Ethanamizuril in chicken under the three dispersants was 4 ~ 5 hours, and the half-life (t1/2) of Ethanamizuril in sodium carboxymethyl cellulose solution (CMC-Na), PEG300 and Ethanamizuril solution diluted by water had a significant difference (P < 0.05) and AUC and Cmax had no significant difference which also showed no significant difference in drug exposure. AUC and Cmax were important indicators of drug exposure in toxicity studies. The study showed that different dispersants had little effect on the evaluation of toxicity studies of the drug.
Satisfaction with Assistive Technology among Users with disabilities: Findings from a Sub-national Study in India
Authors: Senjam Suraj Singh , Manna Souvik , Titiyal Jeewan Singh , Kumar Anil , Kishore Jugal 
Published: August 2023

Abstract

Background
User satisfaction with Assistive Technology (AT) is one of the integral outcomes of successful AT service. The current study aimed to estimate the satisfaction with AT and reasons for dissatisfaction among users with disabilities in India
Methods
A cross-sectional study was conducted in eight districts, representing four zones of India, using the WHO Rapid Assistive Technology Assessment tool. Multi-stage cluster random sampling along with the probability proportional to size technique was used to select smaller administrative units from the larger ones. Satisfaction was reported in terms of assistive products and service delivery.
Results
In total, 8486 participants were surveyed out of 8964 individuals enumerated with a response rate of 94.6%. Around 22.2% (1888) of participants with disabilities reported using AT, out of which 7.8% (147) were dissatisfied with their products. The proportion of respondents with disabilities reporting satisfaction with their products, AT services in terms of assessment and training, and AT repair and maintenance were 92.2%, 88.4%, and 85.2%. However, 12.4% (234) and 14.9% (282) of respondents reported that their AT was not suitable for home and public environments, respectively. Another 13.4% (253) respondents reported that their AT does not help them in executing their daily living activities. Discomfort (56.6%), poor fitting (37.7%), low quality of service (20.7%), and poor aesthetic values (18.9%) were identified as reasons for dissatisfaction. Satisfaction was good for AT received from friends and family but was poor for those received from the public sector.
Conclusion
The study shows that overall satisfaction and suitability with AT were high among users with disabilities, yet few have reported barriers to effective device use and facing challenges in accessing repair and follow-up services.
Neutralization against Omicron sublineages (BA.2/BA.5/BQ.1.1/XBB/XBB.1.5) in bivalent BNT162b2-vaccinated HCWs with or without risk factors, or following BT infection with Omicron
Authors: Amano Masayuki , Otsu Sachiko , Uemura Yukari , Ichikawa Yasuko , Matsumoto Shota , Higashi-Kuwata Nobuyo , Matsushita Shuzo , Shimada Shinya , Mitsuya Hiroaki 
Published: August 2023

Abstract
SARS-CoV-2-BA.4/5-adapted-bivalent-BNT162b2-vaccine (bvBNT), developed in response to the recent emergence of immune-evasive Omicron-variants, has been given to individuals who completed at least 2-doses of the monovalent-BNT162b2-vaccine (mvBNT).
In the present cohort study, we evaluated neutralization-titers (NT50s) against Wuhan-strain (SCoV2Wuhan) and Omicron-sublineages including BA.2/BA.5/BQ.1.1/XBB/XBB.1.5, and vaccine-elicited S1-binding-IgG in sera from participants-vaccinated with 5th-bvBNT following 4th-mvBNT.
The 5th-bvBNT-dose elicited good protective-activity against SCoV2Wuhan with geometric-mean (gMean)-NT50 of 1,966~2,091, higher than the peak-values post-4th-mvBNT, and favorable neutralization-activity against not only BA.5 but also BA.2, with ~3.2-/~2.2-fold greater gMean-NT50 compared to the peak-values post-4th-mvBNT-dose, in participants with or without risk-factors. However, neutralization-activity of sera post-5th-bvBNT-dose was low against BQ.1.1/XBB/XBB.1.5. Interestingly, participants receiving bvBNT following breakthrough (BT) infection during Omicron-wave had significantly enhanced neutralization-activity against SCoV2Wuhan/BA.2/BA.5 with ~4.6-/~6.3-/~8.1-fold greater gMean-NT50, respectively, compared to uninfected participants receiving bvBNT. Sera from BT-infected-participants receiving bvBNT had enhanced neutralization-activity against BQ.1.1/XBB/XBB.1.5 by ~3.8-fold compared to those from the same participants post-4th-mvBNT-dose, and had enhanced gMean-NT50 ~5.4-fold greater compared to those of uninfected-participants’ sera post-bvBNT.
These results suggest that repeated stimulation brought about by exposure to BA.5’s-Spike elicit favorable cross-neutralization-activity against various SARS-CoV-2-variants, and that bvBNT vaccination be administered in particular to the individuals who experienced BT-infection.
Malignant pheochromocytoma with multiple bone metastases: case report and literature review
Authors: Sun Chunyue , Zhao Yonghua , Yang Can , Ren Shaokun , Shen Daqing 
Published: August 2023

Abstract
Background
Pheochromocytomas are neuroendocrine tumors originating from the adrenal medulla, with an incidence of 2–9 cases per million people per year. Malignant pheochromocytomas account for approximately 10% of cases, and they can metastasize to the lymph nodes, bone, liver, and lungs. In this paper, we report a case of a patient with malignant pheochromocytes and multiple bone metastases.
Case presentation
We report a 63-year-old male patient who presented with multiple bone metastases 4 years after the first pheochromocytoma resection. The patient presented with low back pain, and outpatient CT suggested bone destruction and hypodense shadows in the left lamina and transverse process of L1. By reviewing the medical history, we found that the patient had a history of pheochromocytoma. The patient underwent further imaging and tissue biopsy. The patient's history and findings confirmed the presence of multiple bone metastases from pheochromocytoma. The patient underwent lumbar spine surgery and radiotherapy.
Conclusions
Patients with pheochromocytoma should be followed up for a long period for early diagnosis and treatment, which will help them to survive disease-free for a long time.
The effect of self-care education with smart phone applications on the severity of mucositis and infection after stem cell transplantation in leukemia patients
Authors: Jalal Rezaei , Roya Azouji , Masoumeh ZakeriMoghadam 
Published: August 2023

Abstract
Introduction: Side effects after stem cell transplantation are still widespread despite huge advances, and self-care education is of paramount importance in such patients. This study aimed to determine the effect of self-care education using smart phone applications on the post-SCT severity of mucositis and infection in leukemia patients.
Materials and methods: This clinical trial study was conducted on 104 SCT patients with leukemia who were assigned to two intervention and control groups with the blocked randomization method. In the control group, patients received routine care, and self-care education with smart phone applications, in addition to routine care, was provided to the intervention group. The questionnaires for transplantation side effects were completed by the control and intervention groups 2 weeks, 1 month, and 3 months after the start of the intervention. Data were analyzed with Chi-square, Fisher's exact, Mann-Whitney, and Friedman tests using SPSS version 25 software.
Results: The severity of mucositis in patients was significantly different in the two groups at all three post-transplantation time points (2 weeks, 1 month, and 3 months) (p-value = 0.000). Patients' infection levels in the two groups differed significantly at all three post-transplantation time points (2 weeks, 1 month, and 3 months) (p-value = 0.000).
Conclusion: The post-SCT severity of mucositis and infection is influenced by self-care education using smart phone applications.
Clinical trial code: IRCT20100725004443N28
Binding affinity predictions with hybrid quantum-classical convolutional neural networks
Authors: Domingo Laia , Djukic Marko , Johnson Christine , Borondo Florentino 
Published: August 2023

Abstract
Central in drug design is the identification of biomolecules that uniquely and robustly bind to a target protein, while minimizing their interactions with others. Accordingly, precise binding affinity prediction, enabling the accurate selection of suitable candidates from an extensive pool of potential compounds, can greatly reduce the expenses associated to practical experimental protocols. In this respect, recent advances revealed that deep learning methods show superior performance compared to other traditional computational methods, especially with the advent of large datasets. These methods, however, are complex and very time-intensive, thus representing an important clear bottleneck for their development and practical application. In this context, the emerging realm of quantum machine learning holds promise for enhancing numerous classical machine learning algorithms. In this work, we take one step forward and present a hybrid quantum-classical convolutional neural network, which is able to reduce by 20% the complexity of the classical counterpart while still maintaining optimal performance in the predictions. Additionally, this results in a significant cost and time savings of up to 40% in the training stage, which means a substantial speed-up of the drug design process.
Multi-omics joint analysis to explore new therapeutic targets for Crohn's disease
Authors: Wang Songjie , Zhang Zhe , Liu Xia , Wang Dandan , Yao Yao , Feng Baisui 
Published: August 2023

Abstract
Background
Infliximab (IFX), the mainstay of current treatment for moderate-to-severe Crohn's disease (CD), markedly improved clinical symptoms and quality of life for patients. However, not all patients can benefit from IFX. This study aims to identify new therapeutic targets for primary nonresponders through combination of transcriptomic and proteomic analyses.
Methods
The plasma and peripheral leukocytes were collected from seven good responders and four primary nonresponders with CD. To identify differentially expressed genes (DEGs) and proteins (DEPs), we performed reference transcriptome analyses and Olink proteomics analyses by Leukocytes and plasma respectively. Immune-related genes (IRGs) were then intersected with DEGs to screen for the differentially expressed immune-related genes (DEIRGs). We subsequently construct the protein-protein interaction (PPI) network with the DEIRGs. We also investigated the expression of DEGs and DEPs in the intestinal mucosa.
Results
A total of 1170 DEGs were identified, including 533 upregulated and 637 downregulated genes in primary nonresponders. After mapping DEGs to IRGs, 128 DEIRGs were screened. We identified five hub genes in the PPI network mapped by the DEIRGs, namely IL1B, MMP9, CXCL10, CD8A and CCR7 genes. Similarly, the Olink proteomics analysis discovered 13 DEPs. In the intestinal mucosa of primary nonresponders, hub genes IL1B, MMP9, CXCL10, and DEPs SLAFM1, MMP1, CXCL11, HGF, IFN-γ, and CXCL10 were also substantially expressed. Combined analysis of DEGs and DEPs revealed a common enrichment in the IL 17 signaling pathway, with CXCL10 being the only DEGs to overlap.
Conclusions
We first connected the intestinal biopsy series expression matrix to the peripheral blood transcriptome and proteome, confirming CXCL10 as a new therapeutic target. IL1B, MMP9, SLAFM1, MMP1, CXCL11, HGF, and IFN-γ were also deserving of consideration as prospective therapeutic targets.
Real-time Multimodal Sensory Detection Using Widefield Hippocampal Calcium Imaging
Authors: French Chris , Sun Dechuan , Yu Yang , Habibollahi Forough , Unnithan Ranjith 
Published: August 2023

Abstract
The hippocampus is a complex structure that has a major role in learning and memory. It also integrates information from multisensory modalities, supporting a comprehensive “cognitive map” for both spatial and non-spatial information. Previous studies have been limited to real-time spatial decoding, typically using electrodes. However, decoding hippocampal non-spatial information in real-time has not been previously described. Here, we have constructed a real-time optical decoder driven by calcium activity of large neuronal ensembles to decode spatial, visual, and auditory information effectively. Using advanced machine learning techniques, our rapid end-to-end decoding achieves high accuracy and provides a “cognitive translation” method. This method enables the real-time investigation of hippocampal neural coding and allows for direct neural communication with animals and patients affected by functional impairments. The ability to decode multimodal sensory inputs in real-time thus forms the basis for a novel all-optical brain-computer interface.
Facial Emotion Recognition using Deep Learning: Advancements, Challenges, and Future Directions
Authors: Kaur Navneet , Kaur Kanwarpreet 
Published: August 2023

Abstract
The study of facial emotion recognition (FER) holds significant importance within the realm of academic research, since it has wide-ranging ramifications across multiple areas such as mental health assessment and human-computer interaction. This work introduces a novel methodology for FER that integrates Gabor filter-based feature extraction with a deep Convolutional Neural Network (CNN). The utilisation of Gabor filters enables extraction of prominent textural characteristics from facial images, whilst CNNs acquire informative representations to achieve precise emotion classification. The proposed methodology is assessed using the FER2013 dataset and compared with pre-existing methodologies. The findings illustrate the efficacy of our methodology in accurately identifying facial expressions of emotions, emphasising its potential for practical implementation in the fields of mental health research and emotion-sensitive systems. The method demonstrates improved accuracy and resilience by combining Gabor filters and CNNs, showing potential for enhancing mental health evaluation and enabling adaptive human-computer interaction. This study makes a valuable contribution to the fields of health, mental health, and adaptation by advancing the creation of emotion-aware technologies that are designed to address the unique emotional requirements of individuals.
Performance of the MeltPro TB assay as initial test for diagnosis of pulmonary tuberculosis with drug-resistance detection
Authors: Liu Zhi-bin , Cheng Li-ping , Pan Hong-qiu , Wu Xiao-cui , Lu Fu-hui , Cao Jie , Wang Lei , Wei Wei , Chen Hong-yu , Sha Wei , Sun Qin 
Published: August 2023

Abstract
Background
The MeltPro TB assay (MeltPro) is a molecular rapid diagnostic test designed for detecting resistance to antituberculosis drugs. However, the performance of MeltPro as an initial diagnostic test for simultaneously detecting the presence of Mycobacterium tuberculosis (MTB) and drug resistance has not been evaluated. This study aims to assess the performance of MeltPro as initial diagnostic test for simultaneous detection of MTB and drug resistance in clinical samples from patients with presumptive pulmonary tuberculosis (PTB).
Methods
A retrospective analysis was conducted on 1283 patients with presumptive PTB from two clinical centers, out of which 875 were diagnosed with PTB. The diagnostic accuracy of MeltPro, Xpert MTB/RIF (Xpert), and MGIT 960 for PTB detection was evaluated. Rifampicin (RIF), isoniazid (INH), ethambutol (EMB), streptomycin (STR), and fluoroquinolone (FQ) resistance were detected using MeltPro, with Xpert and/or the broth microdilution plate method (MYCOTB) results as references.
Results
For the diagnosis of PTB, MeltPro showed a sensitivity of 69.0%, which was similar to Xpert (72.7%; P > 0.05) and higher than MGIT (58.1%; P < 0.001). The specificity of MeltPro was 97.1%, similar to Xpert (98.0%; P > 0.05). In smear-negative patients, MeltPro's sensitivity was 50.9%, similar to Xpert (56.5%; P > 0.05), and higher than MGIT (33.1%; P < 0.001). Based on Xpert and/or MYCOTB results, MeltPro exhibited a sensitivity and specificity of 98.3% and 99.2%, respectively, for detecting RIF resistance. Based on MYCOTB results, MeltPro's sensitivity for detecting resistance to INH, EMB, STR, and FQ was 96.4%, 89.1%, 97.5%, and 90.3%, respectively, with specificities of 96.0%, 96.0%, 95.2%, and 99.4%, respectively.
Conclusion
The MeltPro TB assay proves to be an effective alternative as the initial test for rapid diagnosis of PTB with drug-resistance detection in clinical practice.