bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute inflammation induces acute megakaryopoiesis with impaired platelet production during fetal hematopoiesis
Authors: Hu Xiaojie , He Yirui , Li Shengwei , Jiang Yue , Yu Renjie , Wu Yi , Fu Xiaoying , Song Yuanbin , Lin Changdong , Shi Jiejun , Li Hua-Bing , Gao Yimeng 
Published: January 2025

ABSTRACT
Hematopoietic development is tightly regulated by various factors. The role of RNA m6A modification during fetal hematopoiesis, particularly in megakaryopoiesis, remains unclear. Here, we demonstrate that loss of m6A methyltransferase METTL3 induces formation of double-stranded RNAs (dsRNAs) and activates acute inflammation during fetal hematopoiesis in mouse. This dsRNA-mediated inflammation leads to acute megakaryopoiesis, which facilitates the generation of megakaryocyte progenitors but disrupts megakaryocyte maturation and platelet production. The inflammation and immune response activate the phosphorylation of STAT1 and IRF3, and upregulate downstream interferon-stimulated genes (ISGs). Inflammation inhibits the proliferation rate of hematopoietic progenitors and further skews the cell fate determination toward megakaryocytes rather than toward erythroid from megakaryocyte-erythroid progenitors (MEPs). Transcriptional-wide gene expression analysis identifies IGF1 as a major factor whose reduction is responsible for the inhibition of megakaryopoiesis and thrombopoiesis. Restoration of IGF1 with METTL3-deficient hematopoietic cells significantly increases megakaryocyte maturation. In summary, we elucidate that the loss of RNA m6A modification-induced acute inflammation activates acute megakaryopoiesis, but impairs its final maturation through the inhibition of IGF1 expression during fetal hematopoiesis.
Cadherins and growth factor receptors – ligand-selective mechano-switches at cadherin junctions
Authors: Vu Vinh , Sullivan Brendan , Hebner Evan , Rahil Zainab , Zou Yubo , Leckband Deborah 
Published: January 2025

ABSTRACT
This study investigated possible mechanisms underlying differences between heterophilic and homophilic cadherin adhesions that influence intercellular mechanics and multicellular organization. Results suggest that homophilic cadherin ligation selectively activates force transduction, such that resulting signaling and mechano-transduction amplitudes are independent of cadherin-binding affinities. Epithelial (E-) and neural (N-)cadherin cooperate with distinct growth factors to mechanically activate force transduction cascades. Prior results have demonstrated that E-cadherin and epidermal growth factor receptor form force-sensitive complexes at intercellular junctions. Here, we show that the reconstitution of N-cadherin force transduction requires the co-expression of N-cadherin and fibroblast growth factor receptor. Mechanical measurements further demonstrated that homophilic ligation initiates receptor tyrosine kinase-dependent force transduction cascades, but heterophilic cadherin ligands fail to activate signaling or generate stereotypical mechano-transduction signatures. The all-or-nothing contrast between mechano-transduction by heterophilic versus homophilic cadherin adhesions supersedes differences in cadherin adhesion strength. This mechano-selectivity impacts cell spreading and traction generation on cadherin substrates. Homophilic ligation appears to be a key that selectively unlocks cadherin mechano-transduction. These findings might reconcile the roles of cadherin recognition and cell mechanics in the organization of multicellular assemblies.
Determinants of viral haemorrhagic fever risk in Africa’s tropical moist forests: A scoping review of spatial, socio-economic, and environmental factors
Authors: Sopbué Kamguem Inès , Kirschvink Nathalie , Wade Abel , Linard Catherine 
Published: January 2025

Background
Viral haemorrhagic fevers (VHFs) are identified by international health authorities as priorities for research and development, as they pose a threat to global health and economy. VHFs are zoonotic diseases whose acute forms in humans present a haemorrhagic syndrome and shock, with mortality rates of up to 90%. This work aims at synthetizing existing knowledge on spatial and spatially aggregable determinants that support the emergence and maintenance of VHFs in African countries covered by tropical moist forest, to better identify and map areas at risk.

Methodology/principal findings
Using the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA-ScR) guidelines, extension for scoping reviews, we searched the PubMed, Embase, CAB Abstracts, and Scopus databases. English and French peer-reviewed documents were retrieved using Boolean logic and keyword search terms. The analysis of 79 articles published between 1993 and 2023 offers a comprehensive overview of the complex interactions among abiotic, biotic, demographic, socio-economic, cultural, and political risk factors in driving the emergence and maintenance of VHFs in African countries covered by tropical moist forests. Human-to-human transmission is mainly driven by socio-economic, political, and demographic factors, whereas zoonotic spillover is determined by almost all groups of factors, especially those of an anthropogenic nature.

Conclusions/significance
Many questions remain unanswered regarding the epidemiology of VHFs in tropical forests. By elucidating spatially relevant determinants which have already been studied, this review seeks to advance VHFs hotspot predictions, risk mapping for disease surveillance and control systems improvement.
Clinical Outcomes in a Multicenter Cohort Involving 919 Patients With Hypertriglyceridemia-Associated Acute Pancreatitis
Authors: Deng Hanzhang , Peng Kaixin , Zhang Liang , Lu Jiongdi , Mei Wentong , Shi Xiaolei , Peng Yunpeng , Xu Kedong , Li Haoxuan , Wang Zheng , Lu Guotao , Wang Gang , Lu Zipeng , Cao Feng , Wen Li ,   for the Chinese Hypertriglyceridemia-associated Pancreatitis Study Group (CHPSG)
Published: January 2025

INTRODUCTION:
Hypertriglyceridemia-associated acute pancreatitis (HTG-AP) is one of the most common etiologies of acute pancreatitis (AP) worldwide. Compared with other etiologies, patients with HTG-AP may develop more severe AP, but previous studies yielded controversial conclusion due to the lack of adequate adjustment for the confounders. Therefore, the aim of this study was to examine the possibility and risk factors of developing severe AP in HTG-AP.

METHODS:
Data from patients with an established diagnosis of AP were collected from January 2013 to December 2023 using a predesigned data collection form and were gathered from 5 tertiary cross-regional centers of China. HTG-AP was defined as serum triglyceride levels >500 mg/dL and excluded other etiologies. The possibility and risk factors of severe AP were assessed by multivariable logistic regressions after adjusting potential confounders. A prediction model was established and validated.

RESULTS:
Between 2013 and 2023, we identified a total of 6,996 patients with AP, of whom 4,378 were included in the final analysis. Compared with other etiologies, patients with HTG-AP had a higher risk of developing severe AP (odds ratio: 1.897; 95% confidence interval: 1.380–2.608; P < 0.001) and organ failure. HTG-AP patients showed higher possibility for developing respiratory and circulation failure but renal failure compared with other etiologies. In HTG-AP patients, risk factors of severe AP included age, fasting blood glucose, white blood cell counts, and presence of pleural effusion. TG level was found not significantly associated with severity in HTG-AP patients. A prediction model incorporating these risk factors demonstrated an area under the curve (AUC) of 0.837 in the training and 0.883 in the testing set, with adequate calibration.

DISCUSSION:
Using a multicenter cross-regional cohort, we demonstrated that HTG-AP had a higher risk of developing severe AP and organ failure. A risk prediction model for predicting severe AP was developed and effectively stratified patients.
Quantitative Change of Hepatitis B Surface Antigen Leading to Final Hepatitis B Surface Antigen Loss in Patients with Chronic Hepatitis B Receiving Nucleos(t)ide Analogs in China
Authors: Zhou Tianhui , Shu Meng , Luo Fangyun , Dong Sijia , Teng Jiaming , Du Yanan , Qiu Hong , Cai Wei 
Published: January 2025

INTRODUCTION:
Loss of hepatitis B surface antigen (HBsAg) is the pivotal component of functional cure in patients suffering from chronic hepatitis B (CHB). The predictive value of quantitative HBsAg (qHBsAg) in HBsAg loss among those undergoing nucleos(t)ide analog (NAs) therapy is an area of ongoing investigation.

METHODS:
A retrospective cohort study using electronic medical records was performed. CHB patients with NAs treatment between January 1, 2012, and December 31, 2020 were enrolled and followed up until discontinuation of NAs, as indicated by a gap more than 12 months in prescription refills, past medical record, or study end. Patients were grouped into NAs treatment-naïve cohort and treatment-experienced cohort. In both cohorts, Cox regression models assessed associations between 12-month reduction in qHBsAg, baseline qHBsAg, and HBsAg loss.

RESULTS:
Overall, 2,627 CHB patients with NAs treatment was identified, including 1,179 in treatment-naïve cohort and 1,448 in treatment-experienced cohort. In treatment-naïve cohort, 9 patients had HBsAg loss (0.51/100 person-years). In treatment-experienced cohort, 30 patients had HBsAg loss (1.03/100 person-years). HBsAg loss was significantly associated with a 0.5–1 log10 (treatment-naïve: adjusted hazard ratio [aHR] 8.06, 95% confidence interval [CI] 1.29–50.40; treatment-experienced: aHR 4.34, 95% CI 1.40–13.47) and >1 log10 qHBsAg decrease (treatment-naïve: aHR 9.19, 95% CI 1.47–57.65; treatment-experienced: aHR 8.02, 95% CI 1.76–36.57) compared with qHBsAg not reduced. HBsAg loss was significantly associated with lower baseline qHBsAg in treatment-experienced cohort, while such difference was not significant in treatment-naïve cohort.

DISCUSSION:
A rapid decline of qHBsAg in 12 months during NAs therapy, as opposed to merely maintaining a low level of qHBsAg, was associated with HBsAg loss.
A patient choice‐driven lifestyle intervention lowers HbA1c in type 2 diabetes: A feasibility study
Authors: Weeldreyer Nathan R. , McEntee Mindy L. , Martin Matthew P. , Lee Chong D. , Marvasti Farshad Fani , Gaesser Glenn A. , Kessler Rodger , Angadi Siddhartha S. 
Published: January 2025

AbstractType 2 diabetes (T2D) is a common metabolic disorder in which only 25% of patients meet management targets. While the primary care setting is positioned to provide lifestyle management education, studies are lacking which integrate behavior interventions in this setting utilizing clinic staff. Thus, we evaluated a 90‐day lifestyle intervention for management of glycemia at a family practice clinic administered by clinic medical assistants. Twenty patients with non‐insulin‐dependent T2D completed a 90‐day intervention driven by patient choices of nutrition and physical activity. Medical assistants were trained by members of the study team and administered the intervention under nurse practitioner supervision. HbA1c trended toward significant reduction 8.59 ± 0.9% to 8.15 ± 1.2% (p = 0.051, 95% CI: −0.88 to 0.003). Modest reductions were observed for waist circumference (115.5 ± 12.6 vs. 112.5 ± 15.2 cm; p = 0.014, 95% CI: −5.66 to −0.26), body weight (97.7 ± 21.9 vs. 95.6 ± 23.9 kg; p = 0.016. 95% CI: −3.84 to −0.31), and BMI (33.7 ± 7.2 vs. 32.8 ± 7.5 kg/m2; p = 0.028, 95% CI: −1.29 to −0.12). This 90‐day, patient choice‐intervention was successful at lowering HbA1c in patients with T2D. Our study is limited by a lack of control group, and results should be interpreted as such. These data have implications for team‐based care models in clinic settings to improve health outcomes in patients with T2D.
Implementation of data transmission mechanism in a Bi-directional optical fiber network
Authors: Gummadi Dinesh Venkat , Karanam Harish Naidu , Samudrala Sivanaga Akshaya , Nampally Harshavardhan , Prasanna Preethi Priya Darshini Sagabala , Sindhwani Manoj , Sachdeva Shippu , Shukla Manoj Kumar 
Published: January 2025

Abstract
Passive optical network (PON) has become the leader in delivering broadband high-speed connectivity. The paper introduces a detailed description of PON, its working principle, key features, and their potential applications. In addition, we discuss the types of PON architectures including GPON, EPON, and XG-PON with merits and demerits. Finally, we discuss the technical considerations of PONs in data transmission mechanisms, network design, and component selection. This paper further demonstrates the use of PON technology via a case study on the design and implementation of a bidirectional optical fiber network. Within this project, we went ahead to demonstrate the use of PON components including optical splitters, transmitters, receivers, and power controllers towards the development of an efficient and functional network structure. It mainly covered the results of the project since the PONs appeared to be a promising solution for obtaining high-performing and reliable broadband services.
The case of romantic relationships: analysis of the use of metaphorical frames with ‘traditional family’ and related terms in political Telegram posts in three countries and three languages
Authors: Höhn Sviatlana 
Published: January 2025

Abstract
Language technology – starting from the handwriting in early ages of humanity and perfectionated in the age of social media and micro-targeting - is used to gain and maintain power, and to rebel against it. Social media such as Telegram provide a safe space for freedom fighters in authoritarian regimes but also offer a stage for extremist, aggressive and manipulative content. This work uses romantic relationships as an example topic. The article discusses how the language used shapes the interpretation of political reports based on literature. Further, this work analyses metaphorical frames around the concepts related to romantic relationships and family in influential news outlets from three countries in three languages. The analysis shows that conservative political actors use similar metaphorical frames for relationship-related concepts such as LGBT and ‘traditional family’, and with similar, manipulative purposes. The results suggest that anti-democratic discourses build on exploitation of social categories rendered subordinate to men in a similar way across conservative movements. However, changes in political leadership can facilitate societal changes towards more open and more democratic societies, but they can also change cultures and traditions to suit power interests.
Chronic pelvic pain treatment understanding what matters: a social media survey
Authors: Johnson Selina , Evans Emma , Hapangama Dharani K 
Published: January 2025

AbstractChronic pelvic pain (CPP) is a debilitating condition that reduces quality of life (QoL). In the United Kingdom, there is currently no standardised treatment pathway for women suffering from CPP. Therefore, it is essential to understand individuals’ concerns regarding CPP, their treatment experiences and what they seek from treatment. To do this, we conducted a two-month social media survey focused on the UK population to explore treatment experiences and identify the factors that people consider important to managing their condition. Of 1,279 respondents, women who completed ≥50% of the questions were included (n = 864; 68%). Results suggest that many women are living with moderate-intensity CPP and experience symptoms for 6 years (average) before receiving a diagnosis. Initially, most women see general practitioners and gynaecologists (90%), with varied care beyond these providers. Using an adapted STarT Back tool, 85% of respondents were classified as medium–high risk of poor outcomes based on physical, psychosocial, and psychological risk. Thematic analysis identified that people desire treatment validation/understanding, self-management, and support to manage pain and QoL. Notably, only 26% of respondents report satisfaction with their healthcare experience, suggesting that current treatment approaches do not address these themes. In conclusion, results suggest that treatment should focus on quality-of-life improvement to enhance CPP treatment outcomes and satisfaction. Findings endorse the need for improved and standardised treatment approaches that address patients’ needs.Lay summaryCPP is persistent pain in the lower abdomen or pelvis for at least 6 months. It is common and affects approximately 1 in 6 women in the UK. To improve treatment, it is important to understand people’s treatment experiences and treatment needs. We conducted a social media survey to understand how people with CPP experience treatment and what they would like from treatment. The survey was posted online for two months (May and June 2023) and received 897 responses. Responses suggested that people experience long waits before receiving help for their pain and that treatment journeys vary greatly. Overall, people reported low treatment satisfaction. People felt that effective treatment should improve pain and QoL. Themes of understanding their pain, knowing how to manage their pain and understanding treatments were identified as important. Clinicians should consider QoL and pain education as part of treatment.
Experiences of Gender-Diverse Youth Living With Chronic Pain
Authors: Boerner Katelynn E. , Fox Danya A. , Du Levi , Metzger Daniel L. , Marshall Sheila , Moore Eva M. , Narang Pam , Wharton Marie-Noelle , Oberlander Tim F. 
Published: January 2025

BACKGROUND
Although sex differences in pain are well documented, little is known regarding the relationship between gender and pain. Gender-diverse youth experience unique pain risk factors, including minority stress exposure, but are underrepresented in research.

OBJECTIVE
Elicit experiences of gender-diverse youth who live with chronic pain.

METHODS
Semistructured interviews were conducted with youth virtually using Zoom. Youth were recruited from a Canadian tertiary care pediatric hospital, community-based clinics, and the general population. Interviews were recorded, transcribed, and analyzed with a patient partner using reflexive thematic analysis, integrating relevant existing theoretical and empirical models for understanding gender and pain, identity development, minority stress, and intersectionality.

RESULTS
The final sample included 19 youth who represented a variety of gender identities and pain conditions and reported accessing a range of types and levels of care. Three themes were identified through qualitative analysis: (1) the fight to legitimize both their pain and gender, (2) the tension between affirming gender and managing pain and the role of gender euphoria as a buffer against pain, and (3) the role of intersecting (eg, neurodiversity and race) identities in understanding gender-diverse youths’ pain experiences.

CONCLUSIONS
In a diverse sample of gender-diverse youth who live with chronic pain, experiences of invalidation and difficulty managing pain were experienced in the context of unique stressors and sources of joy in living as a gender-diverse individual. These results point to the need for more intersectional and affirming pain research and integration of findings into clinical practice.
Predictors and Outcomes of Extubation Failure in Preterm Neonates: A Systematic Review
Authors: Hoff Calegari Lisiane , Goyal Medha , Dutta Sourabh , Mukerji Amit 
Published: January 2025

CONTEXT
Extubation failure (EF) is common in preterm neonates and may be associated with adverse outcomes.

OBJECTIVE
To systematically review and meta-analyze the existing literature on predictors and outcomes of EF in preterm neonates.

DATA SOURCES
MEDLINE, Epub Ahead of Print, In-Process & Other Non-Indexed Citations, Embase (OvidSP), CINAHL (EBSCOHost), and Cochrane Library (Wiley) from 1995 onward. The search strategy was developed by a reference librarian.

STUDY SELECTION
Experimental or observational studies reporting on predictors and/or outcomes related to EF (defined as reintubation within 7 days) in preterm neonates less than 37 weeks were eligible. Predictors included machine learning (ML) algorithms and lung ultrasound (LUS). Main outcome of interest was association of EF with mortality and/or bronchopulmonary dysplasia (BPD).

DATA EXTRACTION
Studies identified by the search strategy were screened based on title and abstract. Data from included studies were extracted independently by 2 authors, along with adjudication of risk of bias. RevMan Web was used to conduct meta-analyses.

RESULTS
Out of 8336 studies screened, 120 were included. Neonates with lower gestational age at birth, birthweight, postmenstrual age, and weight at extubation were more likely to experience EF. Higher level of pre-extubation respiratory support, indicated by lower pre-extubation pH and higher pre-extubation mean airway pressure, fraction of inspired oxygen, and Pco2 were associated with EF risk. ML models showed variable accuracy and lower external validity. LUS may be a promising predictor, though scoring systems varied. EF was associated with higher odds of mortality and/or BPD (pooled odds ratio [OR], 4.7; 95% CI, 2.84–7.76) as well as the individual components of the composite: mortality (pooled OR, 3.87; 95% CI, 2.35–6.36) and BPD (pooled OR, 3.27; 95% CI, 2.54–4.21).

LIMITATIONS
Associations were derived from unadjusted data, precluding a definitive causal relationship between EF and predictors/outcomes.

CONCLUSIONS
Lower gestational and chronological age and higher levels of pre-extubation ventilation support were associated with EF. ML models and LUS scores require further validation in larger studies. EF was associated with mortality and/or BPD.
Health Care Stereotype Threat During the COVID-19 Pandemic Among Sexual and Gender Minority Individuals
Authors: Manning Wendy D. , Dush Claire Kamp , Gustafson Kristen 
Published: January 2025

Objectives. To determine the levels and differentials in health care stereotype threat experienced during the COVID-19 pandemic by individuals with sexual or gender minority identities. Methods. The National Couples’ Health and Time Use Study is a national cross-sectional US population‒ based sample of partnered individuals interviewed during the pandemic between September 2020 and April 2021 with oversamples of sexual and gender minority individuals. The analytic sample consisted of 3614 individuals (n = 2043 heterosexual and n = 1571 sexual minority individuals along with 3489 cisgender and 125 noncisgender people). Results. Individuals with sexual minority identities experienced significantly more health care stereotype threat than heterosexual people. Cisgender women and those with another gender identity experienced significantly more health care stereotype threat than cisgender men. These results persisted after the inclusion of indicators of health conditions, insurance, COVID-19 experience, and sociodemographic factors. Conclusions. Gender and sexual minority‒identifying people reported high levels of worries about health discrimination. Our work suggests that providing positive health care experiences may reduce these worries and offer a potential antidote to sexual and gender minority health disparities that are driven by structural and interpersonal discrimination. These findings support calls for the further education and transformation of health care provision and systems. ( Am J Public Health. 2025;115(3):425–433. https://doi.org/10.2105/AJPH.2024.307926 )