bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pulmonary recruitment maneuver reduces the intensity of post-laparoscopic shoulder pain: a systematic review and meta-analysis
Authors: Deng Xiao , Li Hao , Wan Yantong , Lin Xuemei 
Published: January 2023

Abstract
Background: Post-laparoscopic shoulder pain (PLSP) is a commonplace complication following laparoscopic surgeries. This meta-analysis aimed to investigate whether pulmonary recruitment maneuver (PRM) was beneficial to alleviate shoulder pain after laparoscopic procedures.
Methods: We conducted literature research in electronic database from the date of inception to 31 January 2022. The relevant RCTs were independently selected by two authors, followed by data extraction, risk of bias assessing, and results comparison.
Results: Fourteen studies were included in our study involving 1504 patients, among which 607 patients were offered pulmonary recruitment maneuver (PRM) alone or PRM combined with intraperitoneal saline instillation (IPSI) and 573 patients were treated with passive abdominal compression. The administration of PRM significantly decreased post-laparoscopic shoulder pain score at 12h (MD (95%CI) -1.12(-1.57 to -0.66),801 patients, P<0.00001, I2=88%); at 24h (MD (95%CI) -1.45(-1.74 to -1.16), 1180 patients, P<0.00001, I2=78%) and at 48h (MD (95%CI) -0.97(-1.57 to -0.36),780 patients, P<0.00001, I2=85%).
Conclusion: This systematic review and meta-analysis showed that PRM can reduce the intensity of PLSP. However, more studies may be needed to explore the usefulness of PRM in more types of laparoscopic operations other than gynecological surgery and determine the optimal pressure of PRM or appropriate combination with other measures.
Determination of As(V), Se(VI) and Mo(VI) bonding environments in MgAlFe(III)CO3SO4 Hydrotalcites relevant to Uranium mill neutralization processes and tailings
Authors: Gomez Mario Alberto , Ma Xu , Chen Yiwen , Wang Shaofeng , Pollastri Simone , Aquilanti Giuliana , Cui Yubo , Yao Shuhua , Xiao Tangfu 
Published: January 2023

AbstractThe removal of toxic elements (AsO4, SeO4,and MoO4) from wastewater by Hydrotalcites (HTLCs) has been noted, demonstrated, and implemented during mill neutralization processes of Uranium and other mining industries. Unfortunately, the exact knowledge of how these complex to commonly occurring MgAlFe(III)CO3SO4HTLCs has remained uncertain. In this work, the use of K-edge Extended X-ray Absorption Fine Structure Spectroscopy (EXAFS), Density Functional Theory (DFT), Powder X-ray Diffraction (PXRD), and Vibrational Spectroscopy (Infrared and Raman) were combined to answer this question. Based on this data, it was found that all these toxic elements formed surface outer-sphere like complexes with our MgAlFe HTLC. These results clear up the debate with respect to how these toxic elements interact with the MgAlFe HTLCs produced during such mill neutralization processes to correct current geochemical models and build a fundamental basis to create lacking surface complexation models of HTLCs.
Dynamics of the Middle Ear with an Implantable Hearing Device. An improved Electromechanical Model
Authors: Rusinek Rafal , Weremczuk Andrzej 
Published: January 2023

Abstract
An implantable middle ear hearing device is a promising and novel apparatus that can help improve hearing for patients with both sensorineural and conductive hearing loss. Therefore, it is of key importance to have an adequate mathematical and numerical model for testing. In this paper, a multi-degree-of-freedom lumped parameter model with electromechanical coupling is proposed. The model has been significantly improved compared with the previous one reported in the literature. Periodic vibrations of the stapes are calculated by means of the harmonic balance method and verified numerically. Irregular and chaotic behaviour patterns are found as well as numerical simulations are performed. As a result, the maps of possible regular and irregular solutions are developed, indicating that bistable periodic solutions and subharmonic stapes motion can be observed even for stimulation necessary for normal hearing.
Occurrence and mass loads of N-nitrosamines discharged from different anthropogenic activities in Desheng River, South China
Authors: Xia Jingxuan , Chen Yingjie , Huang Huanfang , Li Haixiang , Huang Dabao , Liang Yanpeng , Zeng Honghu , Chen Wenwen 
Published: January 2023

Abstract
N-nitrosamines are widespread in various bodies of water, which is of great concern due to their carcinogenic risks and harmful mutagenic effects. Livestock rearing, domestic, agricultural, and industrial wastewaters are the main sources of N-nitrosamines in environmental water. However, information on the amount of N-nitrosamines these different wastewaters contribute to environmental water is scarce. Here, we investigated eight N-nitrosamines and assessed their mass loadings in the Desheng River to quantify the contributions discharged from different anthropogenic activities. NDMA (8.5 ± 5.2 ng/L), NMEA (0.1 ± 0.4 ng/L), NDEA (1.2 ± 1.0 ng/L), NPYR (1.6 ± 4.1 ng/L), NMOR (7.0 ± 7.1 ng/L), NPIP (0.3 ± 0.7 ng/L), and NDBA (7.8 ± 3.8 ng/L) were detected. NDMA and NDBA were the dominant compounds contributing 89% and 92% to the total N-nitrosamine concentrations. The mean cumulative concentrations of N-nitrosamines in the livestock rearing area (26 ± 11 ng/L) and industrial area (24 ± 4.8 ng/L) were higher than those in the residential area (16 ± 6.3 ng/L) and farmland area (15 ± 5.1 ng/L). The significant negative correlation between NDEA and dissolved oxygen indicated the light-screening effect. The mean concentration of N-nitrosamines in the tributaries (22 ng/L) was slightly higher than that in the mainstem (17 ng/L), probably due to the dilution effect of the mainstem. However, the mass loading assessment suggested the negligible mass emission of N-nitrosamines into the mainstem from tributaries, which could be due to the small water flow of tributaries. The average mass loads of N-nitrosamines discharged into the mainstem were ranked as the livestock rearing area (742.7 g/d), industrial area (558.6 g/d), farmland area (93.9 g/d), and residential areas (83.2 g/d). In the livestock rearing, residential, and industrial area, NDMA (60.9%, 53.6%, and 46.7%) and NDBA (34.6%, 33.3%, and 44.9%) contributed the most mass loads; NDMA (23.4%), NDEA (15.8%), NPYR (10.1%), NPIP (12.8%), and NDBA (37.8%) contributed almost all the mass loads in the farmland area. Photodegradation amounts of NDMA (0.65 ~ 5.25 µg/(m3·day)), NDEA (0.37 ~ 0.91 µg/(m3·day)), and NDBA (-0.06 ~ 0.66 µg/(m3·day)) were also calculated according to the mass loading. Quantifying the contribution of different anthropogenic activities to the river will provide important information for regional river water quality protection.
Magnetite nanoparticles modulate microbial nitrate reduction pathways: Implications for nitrogen cycle in ferruginous oceans
Authors: Wang Pengcong , Luo Genming , Papineau Dominic , Liu Deng , Wang Hongmei , Li Yi-Liang , Zhu Zongmin 
Published: January 2023

Abstract
Nitrogen (N) is an essential element for living organisms that limits the productivity of biospheres through time. It has been suggested that ferruginous anoxic waters could enhance the fraction of dissimilatory nitrate reduction to ammonium (DNRA) and conserve the biologically available N in the ecosystems. However, the role of mixed-valent iron [Fe(II)-Fe(III)] minerals (e.g., magnetite) in the microbially-mediated N cycle has been largely overlooked. Here we examine the microbial nitrate reduction in the presence/absence of magnetite nanoparticles (NPs) through a series of anaerobic bench experiments with a common denitrifying bacterium-Pseudogulbenkiania sp. strain 2002. Our results show that NH4+ yield is remarkably enhanced by the presence of magnetite NPs; otherwise the reduction products are mainly N2O/N2. Upon microbial nitrate reduction, magnetite NPs are partially oxidized to form a maghemite-magnetite solid solution. These findings demonstrate that magnetite NPs can partially modulate microbial nitrate reduction from denitrification to DNRA. This metabolic shift has significant implications for N retention in ferruginous water bodies that predominated the Precambrian oceans.
Purification of SARS-CoV-2 RBD in Affinity Chromatography Using a Novel Nanobody Ligand
Authors: DI ZHU , Lin Jing , Qiu Mei , Liu Jianghai , Zeng Xin , Li Xinlei 
Published: January 2023

Abstract
In the spike protein of SARS-CoV-2, the receptor-binding domain (RBD) contains multiple dominant neutralizing epitopes and can be used as an antigen for developing COVID-19 vaccines and neutral antibodies. Affinity chromatography is one of the most extensively used methods for rapid one-step protein purification. However, there is a lack of commercially available affinity ligands for RBD purification. Here, we report the rapid isolation of a nanobody suitable for purifying RBD as an affinity ligand from immune phage display libraries. After bio-panning, the enriched clones were sequenced on next-generation sequencing (NGS) platforms and classified into four groups based on the CDRH3 amino acid sequence. The representative sequences with high nanomolar affinities to RBD were further categorized into two groups via epitope binning analysis. Finally, from the two epitope bins, we found that SS3 showed easy elution under a mild eluting condition and could be used as a functional affinity ligand to purify RBD. These results also indicate that categorizing the bio-panned sequences via high-throughput sequencing (HTS) techniques followed by epitope binning represents a fast workflow to select specific binders with desired properties.
Inhibition of proliferation and migration of hepatocellular carcinoma by knockdown of KIF3A via NF-κB signal pathway
Authors: Zhang Xuewei , Dong Mingming , Zheng Guoxing , Zhu Jinhao , An Bang , Zhou Zibin , Bi Yonghao , Sun Meng , Zhang Chuzhao , Lian Junfeng , Tang Shijie , Wang Xinjia , Liu Wenjie 
Published: January 2023

AbstractBackground The up-regulation of KIF3A possibly predicts the dismal prognostic outcome of hepatocellular carcinoma (HCC). The present work is focused on investigating KIF3A’s function in the growth and migration of HCC cells. Methods KIF3A expression and its role in predicting HCC prognosis were assessed using the TCGA and Genotype-Tissue Expression (GTEx) databases. KIF3A detection conditions in HCC patients were studied using an immunohistochemical panel. siKIF3A was created and then transfected into HepG2 HCC cells. Cell proliferation was examined with the use of the EDU and CCK8. Using the scratch wound healing assays, cell migration was assessed. RT-PCR and Western-blot (WB) assays were adopted for evaluating the expression of genes and proteins. Results KIF3A expression increased in HCC tissues as compared to matched non-carcinoma samples, and it was tightly associated with poor survival and risk factors (Ps < 0.05). KIF3A knockdown hindered the proliferation and migration of HCC cells (Ps < 0.05). KIF3A silencing reduced RelA (NF-κBp65) expression, thus, affecting the activity of HCC cells (Ps < 0.05). Conclusion In this study, the oncogene of hepatocellular carcinoma is KIF3A. Silencing KIF3A inhibited HCC cell growth and migration by suppressing the NF-κB signal pathway. KIF3A was identified as a potential new anti-HCC therapeutic target.
Effect of probiotic supplementation on the gut microbiota composition of infants delivered by cesarean section: An exploratory, randomized, open-label, parallel-controlled trial
Authors: Gong Yujiao , Zhong Hui , Wang Jing , Wang Xianggeng , Huang Linsheng , Zou Yutong , Qin Huanlong , Yang Rong 
Published: January 2023

Abstract
Background: Infants born via caesarean section (CS) are at an increased risk of immune-related diseases later in life, potentially due to altered gut microbiota. Recent research has focused on the administration of probiotics in the prevention of gut microbiota dysbiosis in neonates delivered by CS. This study was performed to investigate the effects of probiotic supplementation on the gut microbiota of CS-delivered infants.
Methods: Thirty full-term neonates delivered by CS were randomized into the intervention (supplemented orally with a probiotic containing Bifidobacterium longum, Lactobacillus acidophilus, and Enterococcus faecalis for 2 weeks) and control groups. Stool samples were collected at birth and 2 weeks and 42 days after birth. The composition of the gut microbiota was analyzed using 16S rRNA sequencing technology.
Results: The applied bacterial strains were abundant in the CS-delivered infants supplemented with probiotics. Probiotics increased the abundance of some beneficial bacteria, such as Bacteroides, Acinetobacter, Veillonella, and Faecalibacterium. Low colonization of Klebsiella, a potentially pathogenicbacterium, was observed in the intervention group.
Conclusions: Our results showed that probiotics supplemented immediately after CS enriched the gut microbiota composition and altered the pattern of early gut colonization.
Effect of optimizing the induction regimen of drugs in preventing cough reactions in patients undergoing general anesthesia: a single-center, randomized, controlled trial
Authors: Wang Lei , Lu Xing , Cheng Yi , Zhang You-chuan , Zhao De-xiang , Zhu Yan-hong 
Published: January 2023

Abstract
Backgroud

During the induction of general anesthesia, opioids and endotracheal intubation may cause coughing. This study aimed to determine the safety and clinical effects of an optimized drug induction scheme for general anesthesia to prevent coughing in patients.

Methods

A total of 220 patients aged 18 to 65 years who underwent surgery under general anesthesia with endotracheal intubation were randomly assigned to two groups with 110 cases each; one group was administered with a divided sufentanil bolus (group A) and the other with a single sufentanil bolus (group B). Anesthesia induction was performed according to the drug induction scheme of 0, 1, and 3 min. In group A, intravenous sufentanil was administered at 0.1 µg/kg for 2–3 s; intravenous propofol was administered at 1 min for 20–30 s, rocuronium bromide for 10–15 s, and the remaining sufentanil for 3–5 s after rocuronium bromide administration. In group B, sufentanil was administered intravenously after rocuronium bromide was administered once, and the doses and bolus injection rates of the remaining drugs were the same as those in group A. The primary outcome was a cough reaction caused by opioids. We also recorded the pain associated with drug injection, hemodynamics, and blood oxygen saturation during the induction of general anesthesia.

Results

All patients were included in the statistical analysis. There were 10 cases of sufentanil-induced choking reactions in group A and 0 cases in group B, and the difference was statistically significant (P < 0.05). There was no choking reaction of tracheal intubation in the two groups (both P > 0.05). There was no severe pain due to propofol and rocuronium injection in the two groups (P > 0.05). The mean arterial pressure (MAP), heart rate (HR), and peripheral oxygen saturation (SpO2) values were within the normal range at each time point during the induction period.

Conclusion

Following the concise general anesthesia induction scheme at 0, 1, and 3 min, an optimized general anesthesia induction regimen of rocuronium bromide that was rapidly administered, and a single final intravenous bolus of sufentanil, sufentanil-induced coughing reaction was completely eliminated.

Trial registration:

Chinese Clinical Trial Registry: ChiCTR2200062749 (registration date: 17/08/2022).
Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosis
Authors: Matsubayashi Hideaki , Mountain Jack , Yao Tony , Peterson Amy , Roy Abhijit Deb , Inoue Takanari 
Published: January 2023

Abstract
Class IA phosphoinositide 3-kinase (PI3K) galvanizes fundamental cellular processes such as migration, proliferation, and differentiation. To enable multifaceted roles, the catalytic subunit p110 utilizes a multi-domain, regulatory subunit p85 through its inter SH2 domain (iSH2). In cell migration, their product PI(3,4,5)P3 generates locomotive activity. While non-catalytic roles are also implicated, underlying mechanisms and its relationship to PI(3,4,5)P3 signaling remain elusive. Here, we report that a disordered region of iSH2 contains previously uncharacterized AP-2 binding motifs which can trigger clathrin and dynamin-mediated endocytosis independent of PI3K catalytic activity. The AP-2 binding motif mutants of p85 aberrantly accumulate at focal adhesions and upregulate both velocity and persistency in fibroblast migration. We thus propose the dual functionality of PI3K in the control of cell motility, catalytic and non-catalytic, arising distinctly from juxtaposed regions within iSH2.
Early-Onset Anterior Endplate Fractures of Adjacent Vertebras in Balloon Kyphoplasty and Spinejack®
Authors: Chou KuanNien , Chung Ming-Hsuan , Wang Peng-Wei , Ju Da-Tong 
Published: January 2023

Abstract
Background: Vertebral compression fractures (VCFs) occur most commonly on the thoracolumbar junction, which are the most vulnerable biomechanical regiments at the thoracolumbar junction between the thoracic kyphosis and lumbar lordosis. Percutaneous vertebroplasty and kyphoplasty are currently the primary minimally invasive surgical treatments for thoracolumbar VCFs. The principal aim of this study is to compare the clinical efficacies of percutaneous balloon kyphoplasty (BKP) and SpineJack® (SJ) techniques in the treatment of thoracolumbar VCFs by evaluating the risk factors for early-onset adjacent level fractures (ALFs) over a 6-month follow-up.
Methods: A total of 106 patients with thoracolumbar (T11-L2) VCFs, treated with kyphoplasty techniques (64 with BKP and 42 with SJ), were enrolled in this retrospective study. Patterns of preoperative, postoperative day 1, and postoperative 6-month thoracolumbar spine images and discrepancies of long-term kyphosis correction functions were compared using two different kyphoplasty procedures.
Results: A total of 19 new early-onset ALFs were found within one month postoperatively (9 in BKP and 10 in SJ groups), and all presented as anterior vertebral endplate fractures. The early-onset ALFs in the BKP group were anterior inferior endplate fractures at the superior vertebral levels mainly. The preoperative anterior-type and severe VCFs were the statistically significant risk factors for early-onset ALFs. The timing of kyphoplasty intervention for acute VCFs within 1 month and patients with advance age were also presented risk factors to developed early-onset ALFs in our study (p = 0.018; p < 0.001 ).
Conclusions: There was no superiority in the occurrence rate of early-onset ALFs and kyphosis correction effects of BKP or SJ techniques in the treatment of thoracolumbar VCFs. The factors, including pre-OP anterior-type and severe VCFs, patients with advance ages, and kyphoplasty timing within one month since the appearance of symptoms, are the primary risk factors for subsequent early-onset ALFs after kyphoplasty to thoracolumbar VCFs.
Comparison of sentinel lymph node distribution and lymphatic drainage pathway between high- and low-risk endometrial cancers
Authors: Chen Jiayu , Liaing Sichen , He Miao , Wang Zhiqi , Deng Hao , Zhang Guo , Yang Xin , Liang Xudong , Sun Xiuli , Zhou Rong , Li Xiaowei , Li Yi , Wang Jianliu 
Published: January 2023

Abstract
Purpose
This study aimed to compare the distribution and drainage pathway of sentinel lymph nodes between high- and low-risk endometrial cancers.
Methods
In total, 429 patients with endometrial cancer who underwent sentinel lymph node biopsy in Peking University People’s Hospital from July 2015 to April 2022 were retrospectively enrolled. There were 148 patients in the high-risk group and 281 patients in the low-risk group.
Results
The unilateral and bilateral detection rates of sentinel lymph nodes were 86.5% and 55.9%, respectively. The highest detection rate was achieved in the subgroup with a combined use of Indocyanine green (ICG) and Carbon nanoparticles (CNP) (94.9% for unilateral detection and 66.7% for bilateral detection). The upper paracervical pathway (UPP) was detected in 93.3% of cases in the high-risk group and 96.0% of cases in the low-risk group (p = 0.261). The lower paracervical pathway (LPP) was detected in 10.0% of cases in the high-risk group and 17.9% of cases in the low-risk group (p = 0.048). Remarkably increased detection rates of SLN in the common iliac (7.5%) and para-aortic or precaval areas (2.9%) were observed in the high-risk group. In contrast, a markedly decreased detection rate of SLN in the internal iliac area (1.9%) was observed in the high-risk group.
Conclusion
The combined use of ICG and CNP improved the bilateral detection rate of SLN in the high-risk group. The detection of UPP is important for both high-risk and low-risk cases, while LPP detection plays a more important role in the low-risk group. Lymphadenectomy in the common iliac and para-aortic or precaval areas is essential for patients with high-risk EC. Removal of internal iliac lymph nodes is essential for patients with low-risk EC, in case of ineffective SLN mapping.
Stability of synchronisation manifold in coupled fractional order nonlinear oscillators: a master stability function approach
Authors: Rajagopal Karthikeyan , Jafari Sajad , Duraisamy Prakash , Boulaaras Salah Mahmoud , Karthikeyan Anitha 
Published: January 2023

Abstract
Master stability function (MSF) plays a substantial role in understanding the synchronisation behaviour of coupled nonlinear oscillators. Recent attention in the fractional calculus and its applications in nonlinear dynamics has expanded to investigate the network dynamics of them. Hence, we derive the MSF for couped fractional order nonlinear oscillators and investigate their relation with coupling strength and fractional order. To make the comparison between integer and fractional order MSF, we have used well known nonlinear oscillators for investigation. Similar to the integer order, the fractional order coupled nonlinear oscillators too exhibit MSFs which are analysed for existence of negative with in the finite interval of normalized coupling parameter value. We have used the same classifications of integer order MSFs to define different classes for fractional order MSF’s. By using numerical simulations, we could show that majority of fractional order coupled oscillators exhibit higher classes of MSF confirming better synchronisation compared to their integer order counterparts.