bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non‐specific phospholipase C4 hydrolyzes phosphosphingolipids and phosphoglycerolipids and promotes rapeseed growth and yield
Authors: Yang Bao , Li Jianwu , Yan Jiayu , Zhang Ke , Ouyang Zhewen , Lu Yefei , Wei Huili , Li Qing , Yao Xuan , Lu Shaoping , Hong Yueyun , Wang Xuemin , Guo Liang 
Published: August 2023

ABSTRACTPhosphorus is a major nutrient vital for plant growth and development, with a substantial amount of cellular phosphorus being used for the biosynthesis of membrane phospholipids. Here, we report that NON‐SPECIFIC PHOSPHOLIPASE C4 (NPC4) in rapeseed (Brassica napus) releases phosphate from phospholipids to promote growth and seed yield, as plants with altered NPC4 levels showed significant changes in seed production under different phosphate conditions. Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated nuclease 9 (Cas9)‐mediated knockout of BnaNPC4 led to elevated accumulation of phospholipids and decreased growth, whereas overexpression (OE) of BnaNPC4 resulted in lower phospholipid contents and increased plant growth and seed production. We demonstrate that BnaNPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in vitro, and plants with altered BnaNPC4 function displayed changes in their sphingolipid and glycerolipid contents in roots, with a greater change in glycerolipids than sphingolipids in leaves, particularly under phosphate deficiency conditions. In addition, BnaNPC4‐OE plants led to the upregulation of genes involved in lipid metabolism, phosphate release, and phosphate transport and an increase in free inorganic phosphate in leaves. These results indicate that BnaNPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in rapeseed to enhance phosphate release from membrane phospholipids and promote growth and seed production.
Impact of home‐visit nursing service use on costs in the last 3 months of life among older adults: A retrospective cohort study
Authors: Sakano Tomomi , Anzai Tatsuhiko , Takahashi Kunihiko , Fukui Sakiko 
Published: August 2023

AbstractIntroductionConsidering Japan's aging society, the number of older individuals who die at home is expected to increase. In Japan, there are challenges in utilizing and promoting home‐visit nursing services at the end of life for community‐dwelling older adults. We examined the use of home‐visit nursing services at the end of patients' lives and the recommended use patterns of this service (utilization, timing of initiation, and continuity) that contribute to reducing the medical care and long‐term care costs (total costs) in the last 3 months of life.DesignThis was a retrospective cohort study.MethodsWe examined 33 municipalities in Japan, including depopulated areas. The analysis included 22,927 people aged 75 or older who died between September 2016 and September 2018. We used monthly medical care and long‐term care insurance claims data. Participants were classified into five groups based on their history of home‐visit nursing service use: (1) early initiation/continuous use, (2) early initiation/discontinued or fragment use, (3) not‐early initiation/continuous use, (4) not‐early initiation/fragment use, and (5) no use. Univariate and multivariate linear regression analyses were performed to examine the association between total costs in the last 3 months of life and patterns of home‐visit nursing service use.ResultsOverall, the median age was 85, and 12,217 participants were men (53.3%). In the last half year before death, 5424 (23.7%) older adults used home‐visit nursing services. Multivariable linear regression analysis of the log10‐transformed value of total costs revealed that compared with the no use group, the early initiation/continuous use group was estimated to have 0.88 times (95% confidence interval: 0.84, 0.93) the total costs in the last 3 months of life (p < 0.001).ConclusionEarly initiation use of home‐visit nursing services may contribute to reducing total costs in the last 3 months of life for Japanese people aged 75 years or older living at home as they approach the end of life.Clinical relevanceWhen approaching the end of life, many older adults require daily life care and palliative care. Policymakers are strengthening end‐of‐life care for community‐dwelling older adults in Japan. Although the current results do not demonstrate the effectiveness of home‐visit nursing services, they provide a perspective from which to assess the use of home‐visit nursing services and its impact on older adults. The findings can be helpful in considering how to provide nursing care in home‐care settings for older adults who prefer to spend their final days at home.
Low income is associated with impaired jaw function via anxiety and depression in patients with temporomandibular disorders
Authors: Zheng Yunhao , Zhou Xueman , Huang Yi , Lu Jinjin , Cheng Qiaoyu , Fan Peidi , Xiong Xin 
Published: August 2023

AbstractObjectiveThe association between jaw function and income in patients with temporomandibular disorders (TMDs) remain unclear. The aim of this study was to explore this association and its relationship with anxiety and depression.Subjects and MethodsA total of 451 TMD patients, including 361 males and 90 females, participated in this study. The sociodemographic information of patients and their questionnaires including the Generalised Anxiety Disorder 7‐item (GAD‐7), Patient Health Questionnaire 9‐item (PHQ‐9), and Jaw Functional Limitation Scale‐8 (JFLS‐8) were collected. Patients were divided into the high‐income and low‐income groups based on a household per capita income of 6000 RMB per month. Multiple regression and mediation analysis were used to explore the association between variables. The bootstrap method was applied to estimate confidence intervals (CIs).ResultsHigher JFLS‐8 scores were significantly correlated with higher GAD‐7 scores (r = 0.361, p < .001), PHQ‐9 scores (r = 0.339, p < .001). Females and patients with low income had statistically higher JFLS‐8 scores (p < .01, p < .001). Mediation analysis with 10 000 bootstrap simulations revealed a significant direct association between JFLS‐8 scores and income (−2.920, 95% CI [−4.757, −1.044], p = .002). A significant indirect association of JFLS‐8 scores with income via GAD‐7 scores and PHQ‐9 scores was also observed (−0.889, 95% CI [−1.728, −0.164], p = .025), accounting for 23.3% of the total association.ConclusionsLow income is associated with impaired jaw function via anxiety and depression in patients with TMD. Clinicians may need to pay more attention to the psychological status of low‐income TMD patients in clinical practice.
Tailoring cognitive behavioural therapy for insomnia across contexts, conditions, and individuals: What do we know, where do we go?
Authors: Crowther Meagan E. , Saunders William J. , Sletten Tracey L. , Drummond Sean P. A. , Bei Bei 
Published: August 2023

SummaryCognitive behavioural therapy for insomnia (CBT‐I) is considered the front‐line treatment for insomnia. Despite the demonstrated effectiveness of CBT‐I, it is necessary to consider how CBT‐I may be tailored to different individuals. The purpose of the present review is to provide a summary of literature on tailoring CBT‐I to different individuals and provide directions for future research. This review focused on the following domains of adaptation: (i) tailoring CBT‐I components to individuals with comorbid mental or physical health conditions such as comorbid depression and pain; (ii) adapting CBT‐I delivery for different contexts in which individuals exist, such as inpatient, educational, and different social/cultural settings, (iii) adapting CBT‐I to specific individuals via case‐formulation in clinical settings. We highlight current gaps in the exploration of tailored CBT‐I, including a lack of research methodology to evaluate tailored interventions, a need for the integration of ongoing individualised assessment to inform treatment, and the necessary involvement of consumers and stakeholders throughout the research and treatment development process. Together, this review showed abundant adaptations in CBT‐I already exist in the literature. Future research is needed in understanding when and how to apply adaptations in CBT‐I and evaluate the benefits of these adaptations.
Lost in Translation: A Preliminary Examination of Phonological Processing, Language, and Reading in Childhood Epilepsy
Authors: Bailey Katharine , Im‐Bolter Nancie 
Published: August 2023

ABSTRACTChildren with epilepsy are at increased risk for language and reading difficulties. Phonological processing and language underlie reading success, yet their association with reading achievement in children with epilepsy is unknown. We assessed phonological processing, oral language, and reading in children (ages 6 to 12) with epilepsy (EP; n = 6), language problems (LP; n = 14), and typical development (TD; n = 15). The EP and LP group had lower scores on all measures compared with the TD group, with the EP group scoring the lowest on reading comprehension. Phonological processing followed by epilepsy diagnosis predicted reading decoding. Language followed by epilepsy diagnosis predicted reading comprehension. Thus, reading problems in children with epilepsy are not fully explained by their phonological processing or language deficits. Further research that considers other cognitive processes important for reading (e.g., executive function) in this population may help inform our understanding of their reading success.
Observed trends in scavenging by common bottlenose dolphins (Tursiops truncatus truncatus) in for‐hire fisheries in the eastern U.S. Gulf of Mexico
Authors: Grewal Corie E. , Powell Jessica R. , Horstman Stacey C. , Farmer Nicholas A. , Ayala Oscar , Read Andrew J. 
Published: August 2023

AbstractInteractions between bottlenose dolphin and recreational rod and reel fisheries are a complex issue for resource managers in the United States, which may impact anglers' catch and lead to dangerous situations for scavenging or depredating dolphins. To examine this issue, we analyzed data collected by observers on for‐hire fishing vessels off the eastern U.S. Gulf Coast from 2009 to 2020. A generalized additive model indicated number of anglers, number of discarded fish, proximity to shore, prior scavenging events, type of released fish, and area fished were all significant predictors of scavenging by dolphins. The Florida panhandle had the highest odds of scavenging, while scavenging in the Big Bend and Tampa Bay has increased through time. The Florida panhandle is a well‐known area for illegal feeding of dolphins, suggesting human behavior may be influencing fisheries interactions. Model outputs indicate that dolphins are primarily cuing on fishing activity rather than number of discards, but are more likely to scavenge discards from the family Lutjanidae (snappers), which comprised 40% of observed discards but 80% of scavenging events. This study highlights factors influencing the frequency of dolphin scavenging events, guiding managers and scientists on additional studies and mitigation measures needed to address this issue.
The Phlebotomine sand fly fauna of Switzerland revisited
Authors: Schaffner Francis , Silaghi Cornelia , Verhulst Niels O. , Depaquit Jérôme , Mathis Alexander 
Published: August 2023

AbstractSand flies (Diptera: Psychodidae, Phlebotominae; Newstead, 1911) are widespread in Europe, being particularly common in the Mediterranean region but rare north of the Alps. Thus, Switzerland is an opportune place to investigate the sand fly fauna on both sides of the Alpine crest, in southern sub‐Mediterranean climate and northern oceanic temperate climate. We reinvestigated the Swiss sand fly fauna with the aim to assess changes in composition, altitudinal distribution, abundance and seasonality. Thirty‐eight sites were investigated with light traps and/or interception sticky traps in 4 years. Ninety and 380 specimens were caught by light traps and sticky traps, respectively, at 15 collecting sites. Four species were identified. Phlebotomus mascittii (Grassi, 1908), Phlebotomus perniciosus (Newstead, 1911) and Sergentomyia minuta (Rondani, 1843) were confirmed in Ticino, and P. mascittii for the first time in neighbouring Grisons. Also, Phlebotomus neglectus (Tonnoir, 1921) is for the first time reported, though at a very low density compared to P. perniciosus at the same site. Its presence in Ticino supports the northward spread observed in Italy. Sand flies were detected north of the Alps at one site only, endorsing a historical report. Overall, the low density of P. perniciosus and very low density of P. neglectus suggest that canine leishmaniosis may not be an important disease risk in Switzerland.
Molar‐incisor hypomineralization in a cohort of individuals born with cleft lip and palate
Authors: Wanderley Lacerda Rosa Helena , Filgueiras Vitor Marques , Guedes Mendonça Alice Castro , Vieira Alexandre Rezende 
Published: August 2023

AbstractObjectiveThis study aimed to investigate the frequency of molar‐incisor hypomineralization (MIH) in individuals born with cleft lip and or cleft palate.Settings and SampleThree hundred eighty‐six individuals born with cleft lip and/or palate before orthodontic treatment.MethodsAll the individuals were submitted to a clinical examination and intraoral standardized photos. The registration of MIH was taken by two orthodontists and analysed in association with the cleft type and laterality. The Kruskal–Wallis test and the regression test were used to compare the frequency of molars and incisors affected according to cleft type and laterality, sex and age.ResultsWe found a frequency of 67.87% of MIH in the studied sample. The frequency varied from 25% (in individuals born with cleft palate) to 77% in individuals born with bilateral cleft lip and palate). The number of affected molars was statistically different depending on cleft type and laterality (P < .001‐ Kruskal–Wallis test). Differences were found between individuals born with unilateral cleft lip and palate and unilateral cleft lip and alveolus (P = .03), and with isolated cleft palate (P = .03), and between individuals born with bilateral cleft lip and palate and born with unilateral cleft lip and alveolus (P = .01), and cleft palate (P = .01). Sex (P = .21) and age (P = .36) had no influence on the frequency of MIH. A positive correlation was found between the number of molars affected and incisors affected (P < .001).ConclusionIndividuals born with cleft lip and palate have a higher frequency of MIH, and the complexity of cleft type was associated with the number of affected molars.
Changes in the light environment: Short‐term responses of photosynthesis and metabolism in spinach
Authors: Darko Eva , Gondor Kinga O. , Kovács Viktória , Janda Tibor 
Published: August 2023

AbstractModification in the light environment can induce several changes even within a short time. In this article, light intensity and spectrum‐dependent changes in photosynthetic and metabolic processes were investigated in spinach leaves. Short‐term exposure of the youngest fully developed leaves provided an elevated CO2 assimilation capacity under red light compared with blue or white light, although the electron transport rate was lower. The stomatal opening was mainly stimulated by blue light. These spectrum‐induced changes also depended on light intensity. When white light was used to activate the photosynthesis, the white light showed a similar light response to blue light regarding the electron transport processes and red light in terms of stomatal opening. In contrast, concerning CO2 assimilation characteristics, the white light resembled blue light at low and red light at high light intensities. These results indicate that the photosynthetic processes strongly interact with the light intensity and spectral composition. Furthermore, changes in spectral composition modified the primary metabolic processes as well. Red light induced the sugar accumulation, while more organic acids that belong to the respiration pathway were produced under blue and white lights. These changes occurred even within a short (30 min) time frame. These results also draw attention to the importance of the light environment used during the measurements of the photosynthetic activity of plants and/or sample collections.
Frequent vascular access thrombosis in a patient with end stage kidney disease on hemodialysis
Authors: Kearney David , Leonberg‐Yoo Amanda , Cohen Raphael 
Published: August 2023

AbstractVascular access thrombosis (VAT) is common among patients receiving hemodialysis and leads to missed dialysis treatments, hospitalizations, catheter placement, and graft/fistula abandonment. This article reviews the association between hypercoagulability and VAT and the high prevalence of hypercoagulable states in end‐stage kidney disease (ESKD). This article reviews the role of antithrombotic and anticoagulant medications in preventing VAT. The article concludes by reviewing the unique challenges of using vitamin K antagonists in patients with ESKD.
An outbreak of anaphylactic transfusion reactions to group B plasma and platelets and its possible relationship to Alpha‐Gal syndrome
Authors: Gilstad Colleen W. , Conry‐Cantilena Kathleen , Zarpak Roya , Eder Anne F. 
Published: August 2023

AbstractBackgroundAlpha‐gal syndrome (AGS) is a potentially life‐threatening acquired meat allergy associated with tick bites. The allergen Galactose‐α‐1,3‐Galactose is antigenically similar to the B blood group antigen. B blood group status offers some protection against development of the allergy. Although not preferred practice, transfusion of plasma and platelets from group B donors is believed to be safe for group O recipients.Study Design and MethodsAnaphylactic transfusion reactions were reported for three patients in two Washington DC hospitals from Nov 2022 to February 2023. A chart review was performed for all three patients. Patients or family members were interviewed, and IgE levels to alpha‐gal were measured in two of the three patients.ResultsOne reaction was acutely fatal. All reactions were to blood group B Plasma or Platelets in blood group O recipients. One patient had two prior anaphylactic reactions to group O RBCs and group B Plasma in a previous admission. All patients came from southern Maryland where AGS is an emerging problem. Two patients had histories of tick bites, previously unexplained gastrointestinal complaints, and abnormal elevated levels of IgE to alpha gal. Two patients had cat allergies.DiscussionAGS is an emerging problem which may have implications for blood transfusion practice. Avoidance of blood group B antigen containing components may be prudent in non‐blood group B patients with established AGS. Investigation for AGS should be considered in the evaluation of anaphylactic transfusion reactions.
A detailed mathematical model of the human atrial cardiomyocyte: integration of electrophysiology and cardiomechanics
Authors: Mazhar Fazeelat , Bartolucci Chiara , Regazzoni Francesco , Paci Michelangelo , Dedè Luca , Quarteroni Alfio , Corsi Cristiana , Severi Stefano 
Published: August 2023

AbstractMechano‐electric regulations (MER) play an important role in the maintenance of cardiac performance. Mechano‐calcium and mechano‐electric feedback (MCF and MEF) pathways adjust the cardiomyocyte contractile force according to mechanical perturbations and affects electro‐mechanical coupling. MER integrates all these regulations in one unit resulting in a complex phenomenon. Computational modelling is a useful tool to accelerate the mechanistic understanding of complex experimental phenomena. We have developed a novel model that integrates the MER loop for human atrial cardiomyocytes with proper consideration of feedforward and feedback pathways. The model couples a modified version of the action potential (AP) Koivumäki model with the contraction model by Quarteroni group. The model simulates iso‐sarcometric and isometric twitches and the feedback effects on AP and Ca2+‐handling. The model showed a biphasic response of Ca2+ transient (CaT) peak to increasing pacing rates and highlights the possible mechanisms involved. The model has shown a shift of the threshold for AP and CaT alternans from 4.6 to 4 Hz under post‐operative atrial fibrillation, induced by depressed SERCA activity. The alternans incidence was dependent on a chain of mechanisms including RyRs availability time, MCF coupling, CaMKII phosphorylation, and the stretch levels. As a result, the model predicted a 10% slowdown of conduction velocity for a 20% stretch, suggesting a role of stretch in creation of substrate formation for atrial fibrillation. Overall, we conclude that the developed model provides a physiological CaT followed by a physiological twitch. This model can open pathways for the future studies of human atrial electromechanics.
imageKey points
With the availability of human atrial cellular data, interest in atrial‐specific model integration has been enhanced.
We have developed a detailed mathematical model of human atrial cardiomyocytes including the mechano‐electric regulatory loop. The model has gone through calibration and evaluation phases against a wide collection of available human in‐vitro data.
The usefulness of the model for analysing clinical problems has been preliminaryly tested by simulating the increased incidence of Ca2+ transient and action potential alternans at high rates in post‐operative atrial fibrillation condition.
The model determines the possible role of mechano‐electric feedback in alternans incidence, which can increase vulnerability to atrial arrhythmias by varying stretch levels.
We found that our physiologically accurate description of Ca2+ handling can reproduce many experimental phenomena and can help to gain insights into the underlying pathophysiological mechanisms.