bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Landbird Population Change in the Southeastern United States
Authors: Michel Nicole L. , Demarest Dean , Jones-Farrand Todd , Gleason Jeff , McKnight Keith , Wilson Randy 
Published: May 2023

AbstractBirds and their habitats are facing unprecedented threats from a multitude of threats. Threats to birds, and consequently their population trajectories, vary both across space and among species groups, and greater knowledge of these patterns will help inform bird conservation and management. Here, we adapt existing methods to estimate continental bird population loss to a regional scale. Our objectives were to (1) identify patterns in regional population change (abundance) for 141 species of landbirds breeding in the Southeast; (2) compare these with continental patterns for the same suites of species, and (3) examine whether population-level changes in the Southeast suggest immediate, regional conservation actions. We found that landbird population losses were overall similar in the Southeast and across North America (−21%). Shrikes, nightjars, and swifts experienced the largest proportional losses among families in both regions. Birds associated with early seral and emergent wetland habitats experienced the greatest losses as did partial migrants and species listed on the Birds of Conservation Concern list for southeastern Bird Conservation Regions. Facultative aerial insectivores experienced the greatest losses in both regions, while obligate aerial insectivores increased in the Southeast in contrast to continental declines, due to rapid population growth in Cliff Swallows (Petrochelidon pyrrhonota). Within the Southeast, the greatest bird losses were in Peninsular Florida and Gulf Coastal Prairie, while the Mississippi Alluvial Valley Bird Conservation Region – where extensive reforestation efforts have been undertaken – had the smallest losses. We found clear differences in patterns of landbird population loss between the Southeastern United States and North America, as well as within the Southeast region. Results from these analyses should provide conservation agencies and partnerships with additional information and new perspectives to guide landscape-level planning and on-the-ground bird conservation delivery efforts to help bring back the nearly three billion birds lost across North America since 1970.DisclaimerThe findings and conclusions in this article are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service.
CoCl2triggered pseudohypoxic stress induces proteasomal degradation of SIRT4viapolyubiquitination of lysines K78 and K299
Authors: Hampel Nils , Georgy Jacqueline , Mehrabipour Mehrnaz , Lang Alexander , Scheller Jürgen , Ahmadian Mohammad R. , Floss Doreen M. , Piekorz Roland P. 
Published: May 2023

AbstractSIRT4 comprises together with SIRT3 and SIRT5 the mitochondrially localized subgroup of sirtuins. SIRT4 regulatesviaits NAD+-dependent enzymatic activities mitochondrial bioenergetics, dynamics (mitochondrial fusion), and quality control (mitophagy). Here, we address the regulation of SIRT4 itself by characterizing its protein stability and degradation upon CoCl2-induced pseudohypoxic stress that typically triggers mitophagy. Interestingly, within the mitochondrial sirtuins, only the protein levels of SIRT4 or ectopically expressed SIRT4-eGFP decrease upon CoCl2treatment of HEK293 cells. Co-treatment with BafA1, an inhibitor of autophagosome-lysosome fusion required for autophagy/mitophagy, or the use of the proteasome inhibitor MG132 prevented CoCl2-induced SIRT4 downregulation. Consistent with the proteasomal degradation of SIRT4, the lysine mutants SIRT4(K78R) and SIRT4(K299R) showed significantly reduced polyubiquitination upon CoCl2treatment and were more resistant to pseudohypoxia-induced degradation as compared to SIRT4. Moreover, SIRT4(K78R) and SIRT4(K299R) displayed increased basal protein stability as compared to wild-type SIRT4 when subjected to MG132 treatment or cycloheximide (CHX) chase assays. Thus, our data indicate that stress-induced protein degradation of SIRT4 occurs through two mechanisms, (i)viamitochondrial autophagy/mitophagy, and (ii) as a separate processviaproteasomal degradation within the cytoplasm.
Urbanization and fragmentation interact to drive mutualism breakdown and the rise of unstable pathogenic communities in forest soil
Authors: Tatsumi Chikae , Atherton Kathryn F. , Garvey Sarah , Conrad-Rooney Emma , Morreale Luca L. , Hutyra Lucy R. , Templer Pamela H. , Bhatnagar Jennifer M. 
Published: May 2023

AbstractTemperate forests are particularly threatened by urbanization and fragmentation, with over 20% (120lJ000 km2) of recently urbanized land in the U.S. subsuming natural forests. We leveraged a unique, well-characterized urban-to-rural and forest edge-to-interior gradient to identify the combined impact of these two land use changes - urbanization and forest fragmentation - on soil microbial community in native, remnant forests. We found evidence of mutualism breakdown between trees and their fungal root mutualists (ectomycorrhizal (ECM) fungi) with urbanization, where ECM fungi colonized fewer tree roots and had less connectivity in soil microbiome networks in urban forests compared to rural forests. However, urbanization did not reduce the relative abundance of ECM fungi in forest soils; instead, forest fragmentation alone led to strong reductions in ECM fungal abundance. At forest edges, ECM fungi were replaced by plant and animal pathogens, as well as copiotrophic, xenobiotics-degrading, and nitrogen-cycling bacteria, including nitrifiers and denitrifiers. Urbanization and fragmentation interacted to generate “suites” of microbes, with urban interior forests harboring highly homogenized microbiomes, while edge forests microbiomes were more heterogeneous and less stable, showing increased vulnerability to low soil moisture. When scaled to the regional level, we found that forest soils are projected to harbor high abundances of fungal pathogens and denitrifying bacteria, even in rural areas, due to extreme, widespread forest fragmentation. Our results highlight the potential for soil microbiome dysfunction - including increased greenhouse gas production - in temperate forest regions that are subsumed by urban expansion, both now and in the future.Significance StatementUrbanization and forest fragmentation are increasingly altering Earth’s ecosystems, yet the effects on soil microbiomes, crucial for plant health and climate regulation, remain unclear. Our data indicate that, in forested land, these two combined, compounding stressors reshape the soil microbiome in ways that could lead to more pathogen infections of plants and animals, higher rates of N loss due to denitrification, and the possibility of tree symbiont extinctions. By identifying the specific environmental stressors that lead to these microbiome shifts, our analysis can be used to inform urban development and forest management plans to mitigate impacts on the soil microbiome to sustain environmental quality and the ecosystem services that remnant native forests provide to society in the coming decades.ClassificationBiological Sciences/Ecology
Utility of FRET in studies of membrane protein oligomerization: the concept of the effective dissociation constant
Authors: McKenzie Daniel , Wirth Daniel , Pogorelov Taras V. , Hristova Kalina 
Published: May 2023

ABSTRACTThe activity of many membrane receptors is controlled through their lateral association into dimers or higher order oligomers. While Förster resonance energy transfer (FRET) measurements have been used extensively to characterize the stability of receptor dimers, the utility of FRET in studies of larger oligomers is unclear. Here we show that we can extract an effective equilibrium dissociation constant from FRET measurements for EphA2, a receptor tyrosine kinase (RTK) known to form active oligomers of heterogeneous distributions in response to its ligand ephrinA1-Fc. The newly introduced effective equilibrium dissociation constant has a well-defined physical meaning and biological significance. It denotes the receptor concentration for which half of the receptors are monomeric and inactive, and the other half are associated into oligomers and are active, irrespective of the exact oligomer size. This work illustrates how FRET, along with fluorescence fluctuation techniques which directly measure the oligomer size, can be a very powerful tool in studies of membrane receptor association and signaling in the plasma membrane.
3D Variability Analysis Reveals a Hidden Conformational Change Controlling Ammonia Transport in Human Asparagine Synthetase
Authors: Coricello Adriana , Nardone Alanya. J. , Lupia Antonio , Gratteri Carmen , Vos Matthijn , Chaptal Vincent , Alcaro Stefano , Zhu Wen , Takagi Yuichiro , Richards Nigel G. J. 
Published: May 2023

ABSTRACTHow motions in enzymes might be linked to catalytic function is of considerable general interest. Advances in X-ray crystallography and cryogenic electron microscopy (cryo-EM) offer the promise of elucidating functionally relevant conformational changes that are not easily studied by other biophysical methods. Here we use 3D variability analysis (3DVA) of the cryo-EM map for wild-type (WT) human asparagine synthetase (ASNS) to identify a functional role for the Arg-142 side chain as a gate that mediates ammonia access to a catalytically relevant intramolecular tunnel. Our 3DVA-derived hypothesis is assessed experimentally, using the R142I variant in which Arg-142 is replaced by isoleucine, and by molecular dynamics (MD) simulations on independent, computational models of the WT human ASNS monomer and its catalytically relevant, ternary complex with β-aspartyl-AMP and MgPPi. Residue fluctuations in the MD trajectories for the human ASNS monomer are consistent with those determined for 3DVA-derived structures. These MD simulations also indicate that the gating function of Arg-142 is separate from the molecular events that form a continuous tunnel linking the two active sites. Experimental support for Arg-142 playing a role in intramolecular ammonia translocation is provided by the glutamine-dependent synthetase activity of the R142 variant relative to WT ASNS. MD simulations of computational models for the R142I variant and the R142I/β-aspartyl-AMP/MgPPiternary complex provide a possible molecular basis for this observation. Overall, the combination of 3DVA with MD simulations is a generally applicable approach to generate testable hypotheses of how conformational changes in buried side chains might regulate function in enzymes.
Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing byPseudomonas putida
Authors: Volke Daniel C. , Gurdo Nicolas , Milanesi Riccardo , Nikel Pablo I. 
Published: May 2023

SummaryPseudomonas putida, a soil bacterium widely used for synthetic biology and metabolic engineering, processes glucose through convergent peripheral pathways that ultimately yield 6-phosphogluconate. Such a periplasmic gluconate shunt (PGS), composed by glucose and gluconate dehydrogenases, sequentially transforms glucose into gluconate and 2-ketogluconate. Although the secretion of these organic acids byP.putidahas been extensively recognized, the mechanism and spatiotemporal regulation of the PGS remained elusive thus far. To address this challenge, we have developed a novel methodology for metabolic flux analysis,D-fluxomics, based on deuterated sugar substrates. D-Fluxomics demonstrated that the PGS underscores a highly dynamic metabolic architecture in glucose-dependent batch cultures ofP.putida, characterized by hierarchical carbon uptake by the PGS throughout the cultivation. Additionally, we show that gluconate and 2-ketogluconate accumulation and consumption can be solely explained as a result of the interplay between growth rate-coupled and decoupled metabolic fluxes. As a consequence, the formation of these acids in the PGS is inversely correlated to the bacterial growth rate—unlike the widely studied overflow metabolism ofEscherichia coliand yeast. Our findings, which underline survival strategies of soil bacteria thriving in their natural environments, open new avenues for engineeringP.putidatowards efficient, sugar-based bioprocesses.
High-Dimensional Encoding of Movement by Single Neurons in Basal Ganglia Output
Authors: Zur Gil , Larry Noga , Joshua Mati 
Published: May 2023

AbstractThe Substantia Nigra pars reticulata (SNpr), an output structure of the basal ganglia, has been hypothesized to gate the execution of movements. Previous studies focusing mostly on saccadic eye movements have reported that SNpr neurons are tonically active and either pause or increase their firing during movements, consistent with the gating role. We recorded activity in the SNpr of two monkeys during smooth pursuit and saccadic eye movements. SNpr neurons exhibited highly diverse reaction patterns during pursuit, including frequent increases and decreases in firing rate, uncorrelated responses in different movement directions and in reward conditions resulting in the high dimensional activity of single neurons. These diverse temporal patterns surpassed those in other oculomotor areas in the frontal cortex, basal ganglia, and cerebellum. These results suggest that temporal properties of the responses enrich the coding capacity of the basal ganglia output beyond gating or permitting movement.
What we do know and don’t know about marketing communications on mature consumers
Authors: Yannopoulou Natalia , Manika Danae , Chandrasapth Koblarp , Tajvidi Mina , Wells Victoria 
Published: May 2023

Purpose
Given the increased significance and rapid growth of an ageing population, this review paper aims to define the mature consumer segment chronologically to resolve definitional inconsistencies found in prior marketing communications literature, identify the current state of the marketing communications field in terms of mature consumer research and highlight future research directions on mature consumers for marketing communications academics and practitioners.

Design/methodology/approach
A synthesis of existing marketing communication research on mature consumers (those aged 50+), published in top-tier journals since 1972, is provided. In total, 106 papers were identified in 21 marketing journals.

Findings
Three existing research themes were identified: market segmentation of mature consumers (we ground this theme in three inter-related facets: chronological age, health [physical and neurological] and self-perception of age [also referred to as cognitive age]); attitudes and behaviours of mature consumers; and marketing to mature consumers. This paper also proposes several future research themes: further definition of mature consumers and widening the scope of examination; segmenting mature consumers to account for heterogeneity; information processing of mature consumers cannot use a one-size-fits-all approach; the influence of marketing mix elements on mature consumers; and alternative methodologies to better understand mature consumers.

Research limitations/implications
Recognising the heterogeneity within the chronologically based mature consumer segment, this paper proposes an extended mature consumer definition which includes biological, psychological and social dimensions, as well as life events and life circumstances, rather than biological age alone.

Practical implications
In practical terms, understanding information processing of mature consumers cannot use a one-size-fits-all approach and marketing mix elements may affect behaviour differently within this segment. This will require alternative methodologies to understand these processes fully.

Originality/value
This synthesis of mature consumers research within the marketing communications field provides key research questions for future research to better understand this market segment and its implications for marketing communications, theory development and practice.
Analysis of legal framework solutions to protect retail consumers
Authors: Ge Xiukun 
Published: May 2023

PurposeThis article analyses the existing retail and distribution legal framework in which Internet companies operate, focusing on data breaches and other issues of relevance to these companies. In order to identify who should be responsible for the largest share of improving people's quality of life, this study takes into account the perspectives of both consumers and businesses (or service provider). The author states that where there is a high probability of a security or privacy breach and the customer suffers moderate to severe damage, the burden of proof may shift to the corporation. However, the customer's obligation is conditioned by factors such as the customer's risk tolerance, the customer's losses and the efficiency of the security investment.Design/methodology/approachThe author suggests that the decentralized nature of blockchain, information sharing, immutability and smart contracting capabilities have the potential to disrupt established business models and social norms. Challenges related to trust, customs oversight and payments are discussed, as well as the process of creating the framework for electronic commerce. As part of this research, the author has taken into consideration the increasing popularity of Internet shopping.FindingsThe author demonstrates that due to the worldwide reach of the internet and the fast advancement of computer technology, the economies of the globe have grown increasingly linked. Even though e-commerce has been growing rapidly in recent years due to innovations in both technology and international retail and distribution forms, it still confronts a number of challenges.Research limitations/implicationsIn e-commerce that makes use of blockchain technology, there are significant costs associated with transferring data formats, a lack of consensus and limited emissivity in the flow of law and information. Reduced costs and associated negative externalities would be tremendously beneficial for both private enterprise and forward-thinking public policy.Practical implicationsThis paper examines the potential liability concerns that may arise in the context of electronic transactions should a breach of security or privacy occur, as the author shows from a practical standpoint. Computers, mobile devices, tablets, sensors, smart meters and even autos are just some of the many channels via which data may be sent. It is conceivable for data flows in e-commerce, cloud and the Internet of Things to follow a regular pattern. This may endanger the confidentiality or security of the data. These have evolved into a significant barrier that web stores must overcome.Originality/valueThe author argues that resolving disputes related to the processing of electronic transactions is crucial to the growth of e-commerce businesses since customer happiness is directly correlated with business success.
Pathways for the social impact of research in Barcelona's tourism policy
Authors: Viana-Lora Alba , Nel-lo-Andreu Marta 
Published: May 2023

Purpose
This paper aims to analyse Barcelona City Council's tourism policy documents to detect how, through the influence of research, different pathways are produced to achieve social impact.

Design/methodology/approach
Using the case study approach, a qualitative content analysis is applied to review 31 tourism policy documents of Barcelona City Council.

Findings
The results show that the influence of tourism research on Barcelona City Council's policy documents occurs through the following pathways that drive potential social impact: the development of shared research programmes, joint projects, the creation of information exchange platforms, support for academia, the creation of debates, the founding of institutes, the referencing of scientific articles and studies commissioned directly by the City Council from higher education bodies for implementation in the city.

Originality/value
The originality of this paper is to highlight the social relevance of research and to contribute to raising awareness among researchers. The social impact of research is an under-explored topic in the field of tourism. Moreover, there is little research that conducts this analysis through policy documents.
Conducting Monte Carlo simulations with PLS-PM and other variance-based estimators for structural equation models: a tutorial using the R package cSEM
Authors: Schamberger Tamara 
Published: May 2023

PurposeStructural equation modeling (SEM) is a well-established and frequently applied method in various disciplines. New methods in the context of SEM are being introduced in an ongoing manner. Since formal proof of statistical properties is difficult or impossible, new methods are frequently justified using Monte Carlo simulations. For SEM with covariance-based estimators, several tools are available to perform Monte Carlo simulations. Moreover, several guidelines on how to conduct a Monte Carlo simulation for SEM with these tools have been introduced. In contrast, software to estimate structural equation models with variance-based estimators such as partial least squares path modeling (PLS-PM) is limited.Design/methodology/approachAs a remedy, the R packagecSEMwhich allows researchers to estimate structural equation models and to perform Monte Carlo simulations for SEM with variance-based estimators has been introduced. This manuscript provides guidelines on how to conduct a Monte Carlo simulation for SEM with variance-based estimators using the R packagescSEMandcSEM.DGP.FindingsThe author introduces and recommends a six-step procedure to be followed in conducting each Monte Carlo simulation.Originality/valueFor each of the steps, common design patterns are given. Moreover, these guidelines are illustrated by an example Monte Carlo simulation with ready-to-use R code showing that PLS-PM needs the constructs to be embedded in a nomological net to yield valuable results.