bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased pregnancy rates for recurrent implantation failure cases with embryos developed on autologous endometrial co-culture obtained in mono layer and determination of target patient group
Authors: Sertyel Nazike Semra Sümer , Çolakoğlu Mehmet Cengiz , Horasanlı Jule Eriç , İrez Tülay , Gümüş Meryem 
Published: August 2023

Abstract
Background: Although tremendous improvements have been implemented inconventional embryo culture systems, embryo side-products are still a major concern during extended culture. Although the cause of repeated implantation failure (RIF) seems to be multifactorial, several studies have indicated a possible beneficial role of endometrial co-culture systems on RIF cases via improving embryo quality and viability. This study is undertaken to analyze the cumulative contribution of endometrial co-culture (EC) in such cases compare with conventional culture results and to determine the most effective features to work.
Methods: Overall, 678 cycles in which co-culture with the indication of recurrent implantation failure (n≥3) have been performed were included in the study. Patients were retrospectively grouped according to culture media systems used. In 329 patients (group I) endometrial co-culture has been of choice throughout the culture period and in 349 cycles conventional culture media has been utilized. From pre-zygote stage to embryo transfer, daily embryo growth parameters were scored and clinical pregnancy rates compared between groups according to female age, the number of previous attempts and number of mature oocytes obtained.
Results: No statistically significant difference in main patient and cycle parameters such as female age, male age, BMI, gonadotropin ampoules used, MII oocytes collected and fertilized were found between groups. However, when embryo development characteristics were compared, PN (Pronucleus) arrest, cleavage arrest and 3rd day blastomere number and embryo fragmentation rates.
Conclusios: Our results show that culturing embryos on endometrial monolayer cells seems to increase the cleavage rate and the quality of embryos in recurrent implantation failure cases. Furthermore, by comparing all of the results the main target group of this co-culture patients defined (older than 38 age and higher than 2 items) is very important for identified in order to assure the usefulness of co-culture.
Clove oil down regulates the expression of genes encoding the efflux pump, virulence factors and quorum sensing in MDR Pseudomonas aeruginosa
Authors: El-Banna Tarek , Sonbol Fatma , Sallam Norhan , Al-Madboly Lamiaa 
Published: August 2023

Abstract
Background
The emergence of multi-drug resistant Pseudomonas aeruginosa and development of new resistant phenotypes are considered major public health issues. The present work aims to evaluate clove oil effect as anti-bacterial, anti-virulence, efflux pump and quorum sensing inhibitor.
Results
Susceptibility of P. aeruginosa to antimicrobials and clove oil either alone or combined with fluoroquinolones was tested using agar dilution method. Results revealed that 1/2 MIC of clove oil combined with fluoroquinolones showed synergism in 90% isolates. Effect of sub-MICs of clove oil on biofilm formation and some virulence factors was investigated. A significant reduction in virulence attributes as well as in the expression of efflux pump encoding genes (mexA and mexB) and quorum sensing regulatory genes (lasI, lasR, pqsA, pqsR) was recorded with 1/2 MIC of clove oil. An animal model for wound infection was used to assess the therapeutic effect of clove oil, in vivo. Clove oil reduced the bacterial burden in infected wounds by 92% and caused a decline in cell infiltration in excisional wound skin tissue.
Conclusions
Synergistic combinations between fluoroquinolones and sub-MICs of clove oil can be used to modify the antimicrobial resistance level. Clove oil (1/2 MIC) was reported to significantly decrease the expression of efflux and quorum sensing genes followed by reduction in expression of virulence machinery in P. aeruginosa. Clove oil may be used as an antimicrobial adjuvant in treatment protocols of P. aeruginosa infections by lowering the effective doses of the current antimicrobials.
A Nomogram for Diagnosis of BI-RADS 4 Breast Nodules Based on Three-dimensional Volume Ultrasound
Authors: Jiang Xianping , Chen Chen , Yao Jincao , Wang Liping , Yang Chen , Li Wei , Ou Di , Jin Zhiyan , Liu Yuanzhen , Peng Chanjuan , Wang Yifan , Xu Dong 
Published: August 2023

Abstract

Objective
To investigate whether the use of a nomogram prediction model with automated breast ultrasound (ABUS) can help improve accuracy in the differentiation of benign and malignant Breast Imaging Reporting and Data System (BI-RADS) 4 breast nodules.
Methods
Data were collected for a total of 257 nodules with breast nodules corresponding to BI-RADS 4 who underwent ABUS examination and for whom pathology results were obtained from January 2019 to August 2022. The patients were divided into a benign group (188 cases) and a malignant group (69 cases) using a retrospective study method. Ultrasound imaging features were recorded. Logistic regression analysis was used to screen the clinical and ultrasound characteristics. Using the results of these analyses, a nomogram prediction model was established accordingly.
Results
Age, distance between nodule and nipple, calcification and C-plane convergence sign were independent risk factors that enabled differentiation between benign and malignant breast nodules (all P < 0.05). A nomogram model was established based on these variables. The AUC values for the nomogram model, age, distance between nodule and nipple, calcification, and C-plane convergence sign were 0.86, 0.735, 0.645, 0.697, and 0.685, respectively. Thus, the AUC value for the model was significantly higher than a single variable.
Conclusions
A nomogram based on the clinical and ultrasound imaging features of ABUS can be used to improve the accuracy of the diagnosis of benign and malignant BI-RADS 4 nodules. It can function as a relatively accurate predictive tool for sonographers and clinicians and is therefore clinically useful.
Advances in knowledge statement:
we retrospectively analyzed the clinical and ultrasound characteristics of ABUS BI-RADS 4 nodules and established a nomogram model to improve the efficiency of the majority of ABUS readers in the diagnosis of BI-RADS 4 nodules.
Maximally informative feature selection using Information Imbalance: Application to COVID-19 severity prediction
Authors: Wild Romina , Sozio Emanuela , Margiotta Riccardo Giuseppe , Dellai Fabiana , Acquasanta Angela , Ben Fabio Del , Tascini Carlo , Curcio Francesco , Laio Alessandro 
Published: August 2023

Abstract

Clinical databases typically include, for each patient, many heterogeneous features, for example blood exams, the clinical history before the onset of the disease, the evolution of the symptoms, the results of imaging exams, and many others. We here propose to exploit a recently developed statistical approach, the Information Imbalance, to compare different subsets of patient features and automatically select the set of features which is maximally informative for a given clinical purpose, especially in minority classes. We adapt the Information Imbalance approach to work in a clinical framework, where patient features are often categorical, and are generally available only for a fraction of the patients. We apply this algorithm to a data set of ~ 1,300 patients treated for COVID-19 in Udine hospital before October 2021. Using this approach, we find combinations of features which, if used in combination, are maximally informative of the clinical fate and of the severity of the disease. The optimal number of features, which is determined automatically, turns out to be between 10 and 15. These features can be measured at admission. The approach can be used also if the features are available only for a fraction of the patients, does not require imputation and, importantly, is able to automatically select features with small inter-feature correlation. Clinical insights deriving from this study are also discussed.
Prediction error in implicit adaptation during visually- and memory-guided reaching tasks
Authors: Numasawa Kosuke , Miyamoto Takeshi , Kizuka Tomohiro , Ono Seiji 
Published: August 2023

Abstract
Human movements are acquired by motor adaptation in order to maintain their accuracy. There are two systems in motor adaptation, referred to as explicit or implicit adaptation. It has been suggested that the implicit adaptation is based on the prediction error and has been used in a number of motor adaptation studies. This study aimed to examine the effect of visual memory on prediction error in implicit visuomotor adaptation by comparing visually- and memory-guided reaching tasks. The visually-guided task is thought to be implicit learning based on prediction error, whereas the memory-guided task requires more cognitive processes. We observed the adaptation to visuomotor rotation feedback that is gradually rotated. We found that the adaptation and retention rates were higher in the visually-guided task than in the memory-guided task. Furthermore, the delta-band power obtained by electroencephalography (EEG) in the visually-guided task was increased immediately following the visual feedback. Our results suggest that the prediction error is larger in the visually-guided task, which contributes to updating the internal model to enhance the implicit visuomotor adaptation effectively. In contrast, the prediction error could be attenuated in the memory-guided task due to voluntary and cognitive processes.
Evolutionary diversification and succession of soil huge phages in glacier foreland
Authors: Liao Hu , Li Jian , Wang Yan-Zi , Li Hu , An Xin-Li , Wang Tao , chang ruiying , Zhu Yong-Guan , Su Jian-Qiang 
Published: August 2023

Abstract
Huge phages (genome length > 200 kbp) have been found in diverse habitats worldwide, infecting a variety of prokaryotes. Yet, our understanding of their evolution and adaptation strategy in soil is limited due to the scarcity of soil-derived genomes. Here we carried out a size-fractioned (0.22µm) metagenomic survey across a 130-year chronosequence of a glacier foreland in the Tibetan plateau and discovered 597 novel huge phage populations. Their communities in glacier foreland revealed a distinct pattern between the early (≤ 40 years) and late stages (> 40 years). A significant increasing in the diversity and abundance of huge phages in the late stage following glacier retreat were illuminated, which coincided with soil development and vegetation formation. The phages within the late-stage demonstrated remarkable higher microdiversity, gene flow frequency, and stronger phylogenomic clustering, suggesting that natural selection and genetic drift are key drivers of the huge phage speciation. Alongside the shift in huge phage communities, we noted a functional transition between the early and late stages, contributing to the survival and growth of their hosts. This study unravels the hidden diversity of huge phages in soil and highlights the importance of environmental changes in shaping the composition, evolutionary trajectories and function of huge phage communities.
Why do newborns die? Perspectives of community members and Health care providers in the Lawra Municipality in Upper West Region, Ghana
Authors: Bagrmwin Lawrence , Ziem Bernard , Kobekyaa Francis , Azie Reuben , Dun-Dery Frederick , Ajanaba Asakeboba Philomena , Nukpezah Ruth Nimota 
Published: August 2023

Abstract
BACKGROUND
Neonatal deaths contribute significantly to under-five deaths and are a crucial indicator of a country's socioeconomic development, quality of life, and health status. Neonatal deaths occur both at home and in health facilities. Hence community members' and health workers' perspectives collectively define neonatal survival.
AIM
This study explored the perceived causes of neonatal deaths among health service providers and community members.
METHODS
This study employed a qualitative descriptive design with a purposive sampling method of 30 participants (18 community members and 12 healthcare providers). Data were collected using Focus Group Discussion (FGD), transcribed verbatim, coded and analysed using the content analysis technique.
RESULTS
Three categories emerged to describe the factors perceived to cause newborn deaths at health facilities and in the community. These categories were: (1) human factors (newborns, their mothers/families and health staff); (2) place factors (logistics, equipment and their interaction with newborns); and (3) time factors (delays, transport and referral).
CONCLUSIONS
The study identified differences in perspectives between health workers and community members. These differences can affect interventions in neonatal care. Health authorities are encouraged to engage their communities with geographic-specific factors causing neonatal deaths to identify and understand the influencing factors and take everyday actions towards reducing neonatal deaths.
Long-term Treatment of congenital glaucoma and Turner syndrome associated with DMPK gene mutation
Authors: Guo Anqi , Zhang Weijia , Hong Ying 
Published: August 2023

Abstract
Background: Turner syndrome (TS) is a rare disorder associated with complete or partial deletion of the X chromosome, with clinical manifestations including short stature, hypogonadism, cardiovascular system abnormalities, autoimmune disease, and other systemic abnormalities. TS patients with congenital glaucoma are rare.
Case presentation: A 27-year-old female with the karyotype of (45,X) and the DMPK gene Kc.335T>C(p.M112T) on chromosome 19 was diagnosed with congenital glaucoma and TS, presenting with right eye atrophy, cataract, blue sclera, ptosis, amblyopia, and blue-violet blindness in the left eye. Systematic examination revealed the absence of menstruation, congenital uterine agenesis, hypertension, aortic valve malformation, mild aortic regurgitation, diabetes mellitus, abnormal thyroid function, frequent diarrhea, uneven density of the right posterior patella, left pleural adhesions, left renal atrophy, and congenital webbed neck. Multiple anti-glaucoma surgeries were performed, and the intraocular pressure (IOP) was under control in the left eye.
Conclusion: The case exhibited a rare coexistence of primary congenital glaucoma, Turner syndrome, and a DMPK gene mutation. Long-term treatment can keep IOP stable. The difficulty of congenital glaucoma treatment may be related to a specific type of genetic mutation.
The Practice and Determinants of Ambulance Service Utilization in Pre-Hospital Settings, Jimma City, Ethiopia
Authors: Adem Mohammed Ahmed , Tezera Zewdu Baye , Agegnehu Chilot Desta 
Published: August 2023

Abstract

Introduction:
Pre-hospital treatments and means of transport to arrive at appropriate health centers are as vital as in-hospital care, especially, in developing countries that severely suffer from mortality burden arising from emergency conditions. Yet, being a recent phenomenon in Ethiopia, empirical studies are inadequate with regard the practice and determinants of ambulance service utilization in pre-hospital settings. Hence, this study aimed to assess the ambulance service utilization and its determinants among patients admitted to the emergency departments within the context of pre-hospital care system in public hospitals of Jimma City.
Method
An institutional-based cross-sectional study design was conducted in two public hospitals in Jimma city, from June to July 2022. A systematic sampling technique was used to select 468 sample size. Interviewer-administered questionnaire was used to collect data. Data analysis was done using SPSS version 26.0; descriptive and logistic regressions were done, where statistical significance was determined at p < 0.05.
Results
Ambulance service was rendered to bring about 39.5% (of total sample, 451) patients to hospitals. The distribution of service by severity of illnesses was 48.7% among high, and 39.4% among moderately acute cases. The main determinants of ambulance service utilization were: service time (with AOR, 0.35, 95%CI, 0.2–0.6 for those admitted to ED in the morning, and AOR, 2.36, 95%CI, 1.3–4.4 for those at night); referral source (with AOR, 0.2, 95%CI, 0.1–0.4 among the self-referrals); mental status (with AOR, 1.9, 95%CI, 1-3.5 where change in the level of consciousness is observed); first responder (AOR, 6.3 95%CI, 1.5–26 where first responders were the police, and AOR, 3.4, 95%C1, 1.7–6.6 in case of bystanders); distance to hospital (AOR,0.37, 95%CI, 0.2–0.7 among the patients within ≤ 15km radius); and prior experience in ambulance use (AOR, 4.1,95%CI, 2.4-7).
Conclusion
Although the utilization of ambulance in pre-hospital settings was, generally, good in Jimma City; lower levels of service use among patients in more acute health conditions is problematic. Community-based emergency care should be enhanced to improve the knowledge and use of ambulance services.
Effect of spatial moving structure and topology optimization of the CNC turning machine Tools
Authors: Chan Tzu-Chi , Reddy Sabbella Veera Venkata Satyanarayana , Ullah Aman , roy Bedanta 
Published: August 2023

Abstract
In order to enhance the accuracy and productivity of CNC machine tools, Finite Element Method (FEM) is utilized for analyzing machining operations and machine tool structures. Through this approach, it becomes possible to assess and reduce machining errors, thereby improving the precision of machined parts. Additionally, by subjecting machine tool components to analysis of stress and deformations under real operating conditions, the overall performance and lifespan of the machine tool structures can be augmented. This study provides a comprehensive review of recent published papers focusing on the implementation of finite element methods for the analysis and optimization of CNC machine tool operations and structures. In this study, a virtual model of the Goodway CNC turning machine was used to study the machine's behavior. The first three vibrational modes analyzed through a modal analysis were 108.2Hz, 133.4Hz, and 191.7Hz, which were experimentally validated using modal tests. The design precision was supported using the harmonic response. For an applied external load of 500N on the head chuck, the tool turret's transient response was established through transient analysis at 0.4s. However, after the static analysis, the maximum deformation of the machine at the same external applied load on the tool turret was upheld at 7.6µm. The spatial position analysis rectified the modal performance of the machine at a variety of set working positions. As the principal cause of rigidity originates from the base of the machine, three sets of ground support combinations were claimed to test the machine's rigidity. After the topology optimization analysis was achieved, a better-optimized version of the design is suggested. The old and new models were compared, and an increase of 1.5% in the first three modal frequencies was observed.
Numerical simulation of the effect of urban transportation on the spread of air pollution in the residential part of city
Authors: Shadman Ehsan , Nia Pezhman Bahmani 
Published: August 2023

Abstract
Today, the main focus of human society is on environmental protection, and the observance of environmental standards in order to continue human life on Earth is an important and serious concern. The share of cars in large cities in the production and emission of air pollutants is about 70%, and this share is mainly due to fossil fuels. In the present paper, the behavior of air pollution in a part of the city is simulated by considering the wind pattern using computational fluid dynamics. The K-epsilon turbulence model was employed for the simulation. The governing equations for viscous flow included continuity equation, momentum, and turbulence model equations. CFD (Computational fluid dynamics) modeling provides a good description of flow field variables anywhere in the region with details. The resulting mathematical models and fluid flow patterns can be used in research and development, design, and even engineering economics. This simulation of the environment in the form of environmental models has many benefits, including accuracy and speed of modeling, especially using today's computers. Using the results of this simulation, researchers, authorities and policymakers can test the effects of new programs and ideas without spending time and time in the real world. In fact, by modeling instead of crisis management, you can predict or plan the risk.
Frequency of circulating B cell subpopulations in umbilical cord blood and venous blood
Authors: Goedicke-Fritz Sybelle , Bous Michelle , Weber Regine , Kaiser Elisabeth , Rogosch Tobias , Markert Sabrina , Kerzel Sebastian , Schmidt Tina , Maier Rolf Felix , Zemlin Michael 
Published: August 2023

Abstract
Background
The intrauterine and early extrauterine development represents a “window of opportunity” in the immuno-logical development. The underlying mechanisms are still poorly understood. The aim of this study was to provide reference values B cell subpopulations in cord blood of term newborns, juveniles and in adults to find the spectrum of their physiological age-related variation.
Methods
In this study, we used flow cytometry to evaluate human B lymphocytes and subpopulations in cord blood (n = 10), in peripheral blood from healthy juveniles aged 1 to 17 years (n = 20) and from donors aged 24 to 62 years (n = 10).
Results
Our findings showed increasing frequencies of IgM memory B cells, class-switched memory B cells, marginal zone B cells and plasmablasts, from cord blood to peripheral blood of juveniles and adults. In contrast, the percentage of naïve B cells was higher in newborns than in juveniles and adults. The frequencies of immature B cells were similar were similar in cord blood and peripheral blood of adults. Interestingly, transitional B cells frequencies were similar in cord blood and adults but significantly lower in juveniles.
Conclusions
The frequencies of circulating B cell subpopulation are subject to considerable changes during ontogeny, reflecting overlying effects of maturation and of the acquisition of an adaptive immune memory.
Gait speed and sagittal abdominal diameter: The results from the Handan Eye Study
Authors: Liu Juping , Hao Jie , Zhang Ye , Cao Kai , Li Xiaorong , Wang Ningli 
Published: August 2023

Abstract
The objective was to examine the relationship between GS and BMI, WC, waist-hip ratio (WHR), SAD-heigh ratio (SADHtR), WC-height ratio (WCHtR), SAD-WC ratio (SWR). This was a cross-sectional analysis. 2852 participants were included. Unadjusted analyses were performed to estimate the gender-specific means of GS according to each anthropometric index category. Furthermore, the mean of GS according to SWR interquartile was adjusted by age. Multivariate linear regression for GS was performed by SPSS. After adjustment for age, the negative correlation between SWR and GS was confirmed in overall population (t=-2.977, P = 0.003) and in women (t=-2.509, P = 0.012), not in men. The age-adjusted means of GS were 0.983 (95% CI 0.978–0.989) m/sec in SWR 1st IQR, 0.980 (95% CI 0.974–0.987) m/sec in 2nd IQR, and 0.980 (95% CI 0.973–0.986) m/sec in 3th IQR and 0.969 (95% CI 0.963–0.976) m/sec in 4th IQR. Adjust by serum albumin, hypertension and diabetes, the relationship between GS and SWR was still negative correlation in general population(t=-2.257, P = 0.024). This confirms Higher SWR is associated with lower GS. Age and gender should be considered when we explore the relationship between GS and SWR in older adults.