bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical guideline variability in the diagnosis and care of people at risk for hereditary hematopoietic malignancy syndromes
Authors: Hamidi Adam , Roloff Gregory W. , Shaw Reid , Acevedo Maria , Smith Shaili , Drazer Michael W. 
Published: April 2023

AbstractA growing understanding of the complexities of hematopoietic malignancies necessitates the existence of clinical recommendations that are sufficiently comprehensive. Although hereditary hematopoietic malignancies (HHMs) are increasingly recognized for conferring risk of myeloid malignancy, frequently utilized clinical recommendations have never been appraised for the ability to reliably guide HHM evaluation. We assessed established society-level clinical guidelines for inclusion of critical HHM genes and graded the strength of testing recommendations. We uncovered a substantial lack of consistency of recommendations guiding HHM evaluation. Such heterogeneity in guidelines likely contributes to refusal by payers to support HHM testing, leading to underdiagnoses and lost opportunities for clinical surveillance.
Role of the exercise professional in metabolic and bariatric surgery
Authors: Stults-Kolehmainen Matthew A. , Bond Dale S. , Richardson Laura A. , Herring Louisa Y. , Mulone Bethany , Garber Carol Ewing , Morton John , Ghiassi Saber , Duffy Andrew J. , Balk Ethan , Abolt Charles J. , Howard Matt C. , Ash Garrett I. , Williamson Susannah , Marcon Emilian Rejane , Los Santos Melissa De , Bond Samantha , Huehls Janet , Alowaish Osama , Heyman Nina Brojan , Gualano Bruno 
Published: April 2023

ABSTRACTBackgroundPhysical activity (PA) is important for the long-term health and weight management of patients who undergo metabolic and bariatric surgery (MBS). However, the roles of exercise professionals in MBS settings have not been systematically determined.ObjectivesTo investigate: (1) who are the professionals implementing PA programming in MBS clinical settings; and (2) what patient-centric tasks do they perform?SettingClinical and academic exercise settings worldwide.MethodsThis multimethod study included a scoping review of PA programs in MBS described in the research literature. Data about job tasks were extracted and provided to 10 experts to sort into categories. Cluster analysis was utilized to find the hierarchical structure of tasks. A Delphi process was used to agree on a final model.ResultsThe majority of PA professionals were exercise physiologists in the USA and physiotherapists or other types of exercise professionals elsewhere. Forty-three tasks were identified, the most reported being: supervision of exercise, fitness testing, and exercise prescription. Seven higher-order categories were determined: (1) Exercise-related health assessment, (2) Body composition and physical fitness assessment, (3) Lifestyle physical activity and sedentary behavior assessment, (4) Education, instruction, and prescription, (5) Exercise monitoring, (6) Behavioral counseling and psychosocial support, and (7) Dietary support. The following statements were rated an average of 9.0, classifying them as “imperative”: 1) “Pre- and post-operative PA/exercise guidelines for MBS patients are needed”, 2) “MBS programs need to include PA/exercise as part of multidisciplinary care”.ConclusionsThe expert group reached a consensus on 7 major classifications of job tasks for the exercise professional. It is important for governing medical associations across the world to formally recognize experienced exercise professionals as playing pivotal roles in continuing, multidisciplinary care for MBS patients. These findings also provide evidence-based information in the effort to solidify these positions within the greater context of healthcare.HighlightsResults identified 43 patient-centered job tasks, which divided into 7 major categories.The most common tasks were exercise prescription, supervision, and fitness testing.Job tasks performed varied marginally by the type of exercise professional.Including exercise in MBS patient care was deemed “imperative” by the Expert Group.
The unequal functional redundancy of ArabidopsisINCURVATA11andCUPULIFORMIS2is not dependent on genetic background
Authors: Nadi Riad , Juan-Vicente Lucía , Mateo-Bonmatí Eduardo , Micol José Luis 
Published: April 2023

ABSTRACTThe paralogous genesINCURVATA11(ICU11) andCUPULIFORMIS2(CP2) encode components of the epigenetic machinery in Arabidopsis and belong to the 2-oxoglutarate and Fe (II)-dependent dioxygenase superfamily. We previously inferred unequal functional redundancy betweenICU11andCP2from a study of the synergistic phenotypes of the double mutant and sesquimutant combinations oficu11andcp2mutations, although they represented mixed genetic backgrounds. To avoid potential confounding effects arising from different genetic backgrounds, we generated theicu11-5andicu11-6mutants via CRISPR/Cas genome editing in the Col-0 background and crossed them tocp2mutants in Col-0. The resulting mutants exhibited a postembryonic-lethal phenotype reminiscent of strongembryonic flower(emf) mutants. Double mutants involvingicu11-5and mutations affecting epigenetic machinery components displayed synergistic phenotypes, whereascp2-3did not besidesicu11-5. Our results confirmed the unequal functional redundancy betweenICU11andCP2and demonstrated that it is not allele or genetic background specific. An increase in sucrose content in the culture medium partially rescued the post-germinative lethality oficu11 cp2double mutants and sesquimutants, facilitating the study of their morphological phenotypes throughout their life cycle, which include floral organ homeotic transformations. We thus established that theICU11-CP2module is required for proper flower organ identity.
Single cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging and disease
Authors: Kilfeather Peter , Khoo Jia Hui , Wagner Katherina , Liang Han , Caiazza Maria-Claudia , An Yanru , Zhang Xingju , Chen Xiaoyan , Connor-Robson Natalie , Shang Zhouchun , Wade-Martins Richard 
Published: April 2023

SummaryThe brain is spatially organized and contains unique cell types, each performing diverse functions, and exhibiting differential susceptibility to neurodegeneration. This is exemplified in Parkinson’s disease with the preferential loss of dopaminergic neurons of the substantia nigra pars compacta. Using a Parkinson’s transgenic model, we conducted a single-cell spatial transcriptomic and dopaminergic neuron translatomic analysis of young and old mouse brains. Through the high resolving capacity of single-cell spatial transcriptomics, we provide a deep characterization of the expression features of dopaminergic neurons and 27 other cell types within their spatial context, identifying markers of healthy and aging cells, spanning Parkinson’s-relevant pathways. We integrate gene enrichment and GWAS data to prioritize putative causative genes for disease investigation, identifying CASR as a novel regulator of dopaminergic calcium handling. These datasets (see:spatialbrain.org) represent the largest public resource for the investigation of spatial gene expression in brain cells in health, aging and disease.
Characterization of Tryptophan Oxidation Affecting D1 Degradation by FtsH in the Photosystem II Quality Control of Chloroplasts
Authors: Kato Yusuke , Kuroda Hiroshi , Ozawa Shin-Ichiro , Saito Keisuke , Dogra Vivek , Scholz Martin , Zhang Guoxian , de Vitry Catherine , Ishikita Hiroshi , Kim Chanhong , Hippler Michael , Takahashi Yuichiro , Sakamoto Wataru 
Published: April 2023

Photosynthesis is one of the most important reactions for sustaining our environment. Photosystem II (PSII) is the initial site of photosynthetic electron transfer by water oxidation. Light in excess, however, causes the simultaneous production of reactive oxygen species (ROS), leading to photo-oxidative damage in PSII. To maintain photosynthetic activity, the PSII reaction center protein D1, which is the primary target of unavoidable photo-oxidative damage, is efficiently degraded by FtsH protease. In PSII subunits, photo-oxidative modifications of several amino acids such as Trp have been indeed documented, whereas the linkage between such modifications and D1 degradation remains elusive. Here, we show that an oxidative post-translational modification of Trp residue at the N-terminal tail of D1 is correlated with D1 degradation by FtsH during high-light stress. We revealed thatArabidopsismutant lacking FtsH2 had increased levels of oxidative Trp residues in D1, among which an N-terminal Trp-14 was distinctively localized in the stromal side. Further characterization of Trp-14 using chloroplast transformation inChlamydomonasindicated that substitution of D1 Trp-14 to Phe, mimicking Trp oxidation enhanced FtsH-mediated D1 degradation under high light, although the substitution did not affect protein stability and PSII activity. Molecular dynamics simulation of PSII implies that both Trp-14 oxidation and Phe substitution cause fluctuation of D1 N-terminal tail. Furthermore, Trp-14 to Phe modification appeared to have an additive effect in the interaction between FtsH and PSII core in vivo. Together, our results suggest that the Trp oxidation at its N-terminus of D1 may be one of the key oxidations in the PSII repair, leading to processive degradation by FtsH.Competing Interest StatementThe authors have no conflict of interest, financial or otherwise, in relation to this studyImpact StatementOxidative modification of Tryptophan residues in the reaction center protein D1 may be a key to drive the Photosystem II repair, likely enhancing accessibility of FtsH protease to D1.
Phase-specific stimulation of the human brain with real-time measurement instead of prediction
Authors: Guggenberger Robert , Gebühr Julian-Samuel , Keute Marius , Gharabaghi Alireza 
Published: April 2023

AbstractBackgroundThe responsiveness of the human brain to external input fluctuates. Timing the external perturbation with regard to the oscillatory brain state may improve the intended stimulation effects. However, current brain state-dependent interventions targeting phases of the oscillatory cycle need to apply prediction algorithms to compensate for latencies between measurement and stimulation, and are therefore imprecise.ObjectiveWe investigated the phase-specific precision of a novel non-predictive approach on the basis of integrated real-time measurement and brain stimulation.MethodsApplying a simulation, we estimated the circular standard deviation (SD) to hit 2, 4, 8, 16 or 32 equidistant phase bins of the oscillatory cycle with high precision. Furthermore, we used electroencephalography-triggered transcranial magnetic stimulation in healthy subjects to empirically determine the precision of hitting the targeted phase of the oscillatory cycle for 10 different frequencies from 4Hz to 40Hz using our approach.ResultsThe simulation revealed that SDs of less than 17.6°, 9.7°, 5.1°, 2.5°, and 1.3° were necessary to precisely hit 2, 4, 8, 16, and 32 distinct phase bins of the oscillatory cycle. By completing measurement, signal-processing and stimulation with a round-time of 1ms, our empirical approach achieved SDs of 0.4° at 4Hz to 4.3° at 40Hz. This facilitates selective targeting of 32 phases (at 4Hz), 16 phases (at 8, 12, 16, 20, 24Hz) and 8 phases (at 28, 32, 36, 40Hz), respectively.ConclusionIntegrated real-time measurement and stimulation circumvents the need for prediction and results in more precise phase-specific brain stimulation than with state-of-the-art procedures.
Diversity and abundance of pestiferous fruit flies (Diptera: Tephritidae) infesting cucurbit crops in Morogoro, eastern-central Tanzania
Authors: Tarimo Petronila , Kabota Sija , Mwatawala Maulid , Majubwa Ramadhan , Kudra Abdul , Virgilio Massimiliano , De Meyer Marc 
Published: April 2023

AbstractFruit flies represent a significant threat to cucurbit production. They inflict about 30% to 100% losses on cucurbit crops. The aim of this study was to characterize the community of fruit flies infesting cucurbit crops in Morogoro, Tanzania. We investigate the diversity and abundance of fruit fly species infesting cucumber, watermelon, and squash across the two agroecological zones of the Morogoro using three attractants (Cue Lure, Zingerone and Biolure). The attractants were deployed for 16 weeks from June to November 2020.In total, 12017 fruit fly specimens were collected. Of these, 77.23% were collected from the mountainous and the remaining 22.77% from the plateau zone. All specimens belonged to the generaDacus, ZeugodacusandBactrocera.Zeugodacus cucurbitaewas the most abundant species (77.6%) while the remaining species constituted 22.4%. The mountainous zone harboured a significantly higher number of fruit fly species than the plateau zone. The abundance of fruit flies was significantly influenced by altitudes and seasons. The study characterized the community of fruit flies in Morogoro and confirms the prevalence of Z.cucurbitae, D. bivittatus and D. punctatifronsas prominent species in this region. Therefore, management programs should focus on containing these species regardless of the agroecological zones and seasons.
Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons
Authors: Nishida Kazuhiko , Matsumura Shinji , Uchida Hitoshi , Abe Manabu , Sakimura Kenji , Badea Tudor Constantin , Kobayashi Takuya 
Published: April 2023

AbstractThe spinal dorsal horn comprises heterogeneous neuronal populations, that interconnect with one another to form neural circuits modulating various types of sensory information. Decades of evidence has revealed that transcription factors expressed in each neuronal progenitor subclass play pivotal roles in the cell fate specification of spinal dorsal horn neurons. However, the development of subtypes of these neurons is not fully understood in more detail as yet and warrants the investigation of additional transcription factors. In the present study, we examined the involvement of the POU domain-containing transcription factor Brn3a in the development of spinal dorsal horn neurons. Analyses of Brn3a expression in the developing spinal dorsal horn neurons in mice demonstrated that Brn3a was downregulated in majority of the Brn3a-lineage neurons during embryonic stages (Brn3a-transient neurons), whereas a limited population continued to express Brn3a after E18.5 (Brn3a-persistent neurons). Brn3a knockout disrupted the localization pattern of Brn3a-persistent neurons, indicating a critical role of this transcription factor in the development of these neurons. In contrast, Brn3a overexpression in Brn3a-transient neurons directed their localization in a manner similar to that in Brn3a-persistent neurons. Moreover, Brn3a-overexpressing neurons exhibited increased axonal extension to the ventral and ventrolateral funiculi, where the axonal tracts of Brn3a-persistent neurons reside. These results suggest that Brn3a controls the soma localization and axonal extension patterns of Brn3a-persistent spinal dorsal horn neurons.
Persistently increased post-stress activity of paraventricular thalamic neurons is essential for the emergence of stress-induced maladaptive behavior
Authors: Jász Anna , Biró László , Buday Zsolt , Király Bálint , Szalárdy Orsolya , Horváth Krisztina , Komlósi Gergely , Bódizs Róbert , Kovács Krisztina J. , Diana Marco A. , Hangya Balázs , Acsády László 
Published: April 2023

ABSTRACTTraumatic events can immediately lead to debilitating symptoms collectively called Acute Stress Disorder (ASD), however the mechanisms of ASD are poorly understood. Using a rodent model of ASD here we identify a crucial communication bottleneck between the brainstem and the forebrain, the calretinin-positive neurons in the paraventricular thalamus (PVT/CR+), that controls ASD. We show that following a single acute stress event, the pre-sleep behavior of the mice is altered for several days in parallel with a persistent increase in the firing rate of PVT/CR+ cells. Optogenetic inhibition of PVT/CR+ neuronal activity after the stress event for one hour was sufficient to rescue both the ASD symptoms and the prolonged increase in PVT/CR+ firing rate. Inhibition applied 5 days later was still able to ameliorate some of the symptoms. These data suggest that post-stress activity of PVT/CR+ neurons play a critical role in mediating the acute forms of stress-related affective dysfunctions.One-Sentence SummaryPost-stress inhibition of paraventricular thalamic neurons prevents the emergence of acute stressed phenotype.
Enhancer evolution as a driving force for lineage-specific paralog usage in the central nervous system
Authors: Fujimori Chika , Sugimoto Kohei , Ishida Mio , Yang Christopher , Kayo Daichi , Tomihara Soma , Sano Kaori , Akazome Yasuhisa , Oka Yoshitaka , Kanda Shinji 
Published: April 2023

AbstractExpression patterns of paralogous genes in the functionally homologous cells sometimes show differences across species. However, no reasonable explanation for the mechanism underlying such phenomena has been discovered. To understand this mechanism, the present study focused on the hypophysiotropic GnRH neurons in vertebrates as a model. These neurons express eithergnrh1orgnrh3paralogs depending on species, and apparent switching of the expressed paralogs in them occurred at least four times in vertebrate evolution. First, we found redundant expressions ofgnrh1andgnrh3in a single neuron in piranha and hypothesized that this situation may indicate an ancestral condition. We tested this hypothesis by examining the activity of piranhagnrh1/gnrh3enhancers in zebrafish and medaka, in which the twognrhparalogs are not co-expressed. Here, thegnrh1/gnrh3enhancer of piranha induced reporter RFP/GFP co-expressions in a single hypophysiotropic GnRH neuron in both zebrafish and medaka. From these results, we propose that long-lasting (∼550 My) redundancy aftergnrh1/3duplication in 1R/2R WGD may be the key to apparent switching of the paralog usage among the present-day species. Moreover, interspecies analyses of enhancers indicated that the loss of enhancers rather than changes in trans-regulatory elements drove the role-division of these paralogs.
Dysregulation of GABAergic System in the Hippocampus and Inferior Colliculi of Rats during the Development of Audiogenic Epilepsy
Authors: Ivlev Andrey P. , Chernigovskaya Elena V. , Nikolaeva Svetlana D. , Kulikov Alexey A. , Glazova Margarita V. , Naumova Alexandra A. 
Published: April 2023

AbstractEpilepsy is tightly associated with dysfunction of inhibitory γ-aminobutyric acid (GABA) neurotransmission. In this study, Krushinsky-Molodkina (KM) rats genetically prone to audiogenic seizures (AGS) were used. KM rats are characterized by the development of audiogenic epilepsy during postnatal ontogenesis, with AGS onset at the age of 1.5-2 months and fully developed AGS expression by 3rdmonth. We analyzed GABAergic system of the inferior colliculi (IC) and the hippocampus of KM rats at different stages of postnatal development. Wistar rats were used as a control. In the IC of young KM rats, Na+/K+/Cl−cotransporter 1 (NKCC1) expression was increased, while in adults K+/Cl−cotransporter 2 (KCC2) was downregulated indicating impairment of postsynaptic GABA action both at early and later stages of postnatal development. In the hippocampus of young KM rats, we observed a decrease in activity of GABAergic neurons and downregulation of KCC2 and NKCC1. Adult rats, in opposite, demonstrated elevated activity of the hippocampal GABAergic cells and unchanged expression of chlorine transporters indicating upregulation of GABA transmission. Thus, GABA dysregulation in the IC can mediate the seizure susceptibility in adult KM rats, while in the hippocampus, upregulation of GABAergic system can restrict the spreading of epileptiform activity from the brainstem.
MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport inMethanosarcina acetivorans
Authors: Gupta Dinesh , Chen Keying , Elliott Sean J. , Nayak Dipti D. 
Published: April 2023

AbstractMethanogens are a diverse group of Archaea that couple energy conservation to the production of methane gas. While most methanogens have no alternate mode of energy conservation, strains likeMethanosarcina acetivoransare known to also conserve energy by dissimilatory metal reduction (DSMR) in the presence of soluble ferric iron or iron-containing minerals. The ecological ramifications of energy conservation decoupled from methane production in methanogens are substantial, yet the molecular details are poorly understood. In this work, we conductedin vitroandin vivostudies with a multihemec-type cytochrome (MHC), called MmcA, to establish its role during methanogenesis and DSMR inM. acetivorans. MmcA purified fromM. acetivoranscan donate electrons to methanophenazine, a membrane-bound electron carrier, to facilitate methanogenesis. In addition, MmcA can also reduce Fe(III) and the humic acid analog anthraquinone-2,6-disulfonate (AQDS) during DSMR. Furthermore, mutants lackingmmcAhave slower Fe(III) reduction rates. The redox reactivities of MmcA are consistent with the electrochemical data where MmcA displays reversible redox features ranging from -100 to -450 mV versus SHE. MmcA is prevalent in members of the OrderMethanosarcinalesbut does not belong to a known family of MHCs linked to extracellular electron transfer, bioinformatically, and instead forms a distinct clade that is closely related to octaheme tetrathionate reductases. Taken together, this study shows that MmcA is widespread in methanogens with cytochromes where it acts as an electron conduit to support a variety of energy conservation strategies that extend beyond methanogenesis.
Comparative genomics of pesticide-degradingEnterococcussymbionts ofSpodoptera frugiperda(Lepidoptera: Noctuidae) leads to the identification of two new species and the reappraisal of insect-associatedEnterococcusspecies
Authors: Gomes Ana Flávia Freitas , de Almeida Luís Gustavo , Cônsoli Fernando Luis 
Published: April 2023

AbstractEnterococcusspecies have been described as core members of the microbial community ofSpodoptera frugiperda(Lepidoptera:Noctuidae) and have been reported in previous studies as insecticide degrading agents. Phenotypic assays and comparative genomics analyses of several pesticide-degradingEnterococcusisolated from the larval gut ofS. frugiperdaled to the identification ofEnterococcus entomosociumn. sp. andEnterococcus spodopteracolusn. sp. Their identities as new species were confirmed by whole genome alignment using the cut-offs of 95-96% for the average nucleotide identity (ANI) and 70% for the digital DNA:DNA hybridization (dDDH) values. The systematic positioning of these new species within the genusEnterococcuswas resolved using genome-based analysis, placingEnterococcus casseliflavusas the sister group ofE. entomosociumn. sp., andEnterococcus mundtiiofE. spodopteracolusn. sp. Comparative genomic analyses of several isolates ofE. entomosociumn. sp. andE. spodopteracolusn. sp. led to a better assessment of the interactions established in the symbiotic association withS. frugiperda, and the discovery of misidentified new species ofEnterococcusassociated with insects. Our analyses also indicated the potential ofE. entomosociumn. sp. AndE. spodopteracolusn. sp. to metabolize different pesticides arises from molecular mechanisms that result in the rapid evolution of new phenotypes in response to environmental stressors; in this case, the pesticides their host insect is exposed to.