bluuebunny/crossref_metadata_2025_split huggingface dataset:
A structured, telephone-delivered intervention to reduce methamphetamine use: study protocol for a parallel-group randomised controlled trial
Authors: Lubman Dan I. , Manning Victoria , Arunogiri Shalini , Hall Kate , Reynolds John , Stragalinos Peta , Petukhova Rachel , Gerhard Robyn , Tyler Jonathan , Bough Anna , Harris Anthony , Grigg Jasmin 
Published: March 2023

AbstractBackgroundAustralia has one of the highest rates of methamphetamine (MA) use in the world; however, uptake of in-person psychological treatment remains extremely low due to numerous individual (e.g. stigma, shame) and structural (e.g. service accessibility, geographical location) barriers to accessing care. Telephone-delivered interventions are ideally placed to overcome many of the known barriers to treatment access and delivery. This randomised controlled trial (RCT) will examine the efficacy of a standalone, structured telephone-delivered intervention to reduce MA problem severity and related harms.MethodsThis study is a double-blind, parallel-group RCT. We will recruit 196 ± 8 individuals with mild to moderate MA use disorder from across Australia. After eligibility and baseline assessments, participants will be randomly allocated to receive either the Ready2Change-Methamphetamine (R2C-M) intervention (n= 98 ± 4; four to six telephone-delivered intervention sessions, R2C-M workbooks and MA information booklet) or control (n= 98 ± 4; four to six ≤5-min telephone check-ins and MA information booklet including information on accessing further support). Telephone follow-up assessments will occur at 6 weeks and 3, 6 and 12 months post-randomisation. The primary outcome is change in MA problem severity (Drug Use Disorders Identification Test, DUDIT) at 3 months post-randomisation. Secondary outcomes are as follows: MA problem severity (DUDIT) at 6 and 12 months post-randomisation, amount of methamphetamine used, methamphetamine use days, methamphetamine use disorder criteria met, cravings, psychological functioning, psychotic-like experiences, quality of life and other drug use days (at some or all timepoints of 6 weeks and 3, 6 and 12 months post-randomisation). Mixed-methods program evaluation will be performed and cost-effectiveness will be examined.DiscussionThis study will be the first RCT internationally to assess the efficacy of a telephone-delivered intervention for MA use disorder and related harms. The proposed intervention is expected to provide an effective, low-cost, scalable treatment for individuals otherwise unlikely to seek care, preventing future harms and reducing health service and community costs.Trial registrationClinicalTrials.govNCT04713124. Pre-registered on 19 January 2021.
The prevalence of hip osteoarthritis: a systematic review and meta-analysis
Authors: Fan Zijuan , Yan Lei , Liu Haifeng , Li Xiaoke , Fan Kenan , Liu Qiang , Li Jiao Jiao , Wang Bin 
Published: March 2023

AbstractObjectiveTo estimate the global prevalence of hip osteoarthritis (HOA) through a systematic review and meta-analysis, and to determine by regression analysis the respective relationships between age and sex, and sex and prevalence.MethodsEMBASE, PubMed, Web of science, CINAHL, and SCOPUS were searched from inception until August 2022. Two authors independently extracted data and assessed the quality of the retrieved literature. Random-effects meta-analysis was performed to derive the pooled prevalence. Variations in the prevalence estimate in different subgroups, including diagnostic methods, region, and patient sex, were examined by subgroup meta-analysis. Meta-regression was used to construct the age-specific prevalence of HOA.ResultsA total of 31 studies were included in our analysis, involving 326,463 participants. Quality evaluation showed that all studies included in the analysis had a Quality Score of at least 4. The most frequently used method for diagnosing HOA was the Kellgren–Lawrence (K-L) grade classification, accounting for 19/31 (61.3%) studies. The pooled prevalence of HOA diagnosed based on the K-L grade ≥ 2 criterion was 8.55% (95% CI 4.85–13.18) worldwide. The prevalence of HOA was lowest in Africa at 1.20% (95% CI: 0.40–2.38), followed by Asia at 4.26% (95% CI 0.02–14.93) and North America at 7.95% (95% CI 1.98–17.36), and highest in Europe at 12.59% (95% CI 7.17–19.25). There was no statistically significant difference in HOA prevalence between men (9.42%, 95% CI:4.81–15.34) and women at (7.94%, 95% CI: 3.57–13.81). The regression model showed a correlation between age and the prevalence of HOA.ConclusionHOA has high prevalence worldwide and increases with age. The prevalence varies significantly by region but not by patient sex. High-quality epidemiological studies are warranted to more accurately estimate the prevalence of HOA.
Clinical characteristics and prognostic significance of DNA methylation regulatory gene mutations in acute myeloid leukemia
Authors: Xu Xiaoyan , Wang Hong , Han Haohao , Yao Yifang , Li Xueqian , Qi Jiaqian , Cai Chengsen , Zhou Meng , Tang Yaqiong , Pan Tingting , Zhang Ziyan , Yang Jingyi , Wu Depei , Han Yue 
Published: March 2023

Abstract
Background
DNA methylation is a form of epigenetic modification that regulates gene expression. However, there are limited data on the comprehensive analysis of DNA methylation regulated gene mutations (DMRGM) in acute myeloid leukemia (AML) mainly referring to DNA methyltransferase 3α (DNMT3A), isocitrate dehydrogenase 1 (IDH1), isocitrate dehydrogenase 2 (IDH2), and Tet methylcytidine dioxygenase 2 (TET2).

Results
A retrospective study of the clinical characteristics and gene mutations in 843 newly diagnosed non-M3 AML patients was conducted between January 2016 and August 2019. 29.7% (250/843) of patients presented with DMRGM. It was characterized by older age, higher white blood cell count, and higher platelet count (P < 0.05). DMRGM frequently coexisted with FLT3-ITD, NPM1, FLT3-TKD, and RUNX1 mutations (P < 0.05). The CR/CRi rate was only 60.3% in DMRGM patients, significantly lower than in non-DMRGM patients (71.0%, P = 0.014). In addition to being associated with poor overall survival (OS), DMRGM was also an independent risk factor for relapse-free survival (RFS) (HR: 1.467, 95% CI: 1.030–2.090, P = 0.034). Furthermore, OS worsened with an increasing burden of DMRGM. Patients with DMRGM may be benefit from hypomethylating drugs, and the unfavorable prognosis of DMRGM can be overcome by hematopoietic stem cell transplantation (HSCT). For external validation, the BeatAML database was downloaded, and a significant association between DMRGM and OS was confirmed (P < 0.05).

Conclusion
Our study provides an overview of DMRGM in AML patients, which was identified as a risk factor for poor prognosis.
Invest in the future: “Hands-on Radiology” summer school
Authors: Segger Laura , Marth Adrian A. , Gosch Vitus , Oppenheimer Jonas , Lüken Sophia , Bierbrauer Annika , Nilssen Martine S. , Jahn Mona , Hamm Bernd , Lerchbaumer Markus , Auer Timo A. 
Published: March 2023

Abstract
Purpose
The field of radiology is currently underestimated by undergraduate medical students. The “Hands-on Radiology” summer school was established to improve radiology knowledge and interest among undergraduates. The purpose of this questionnaire survey was to analyze whether a radiological hands-on course is an effective tool to reach and motivate undergraduate students.

Materials and methods
The three-day course held in August 2022 included lectures, quizzes, and small group hands-on workshops focusing on practical work with simulators. All participants (n = 30) were asked to rate their knowledge and motivation to specialize in radiology at the beginning of the summer school (day 1) and the end (day 3). The questionnaires included multiple choice questions, 10-point scale questions and open comment questions. The second questionnaire (day 3) included additional questions regarding the program (topic choice, length, etc.).

Results
Out of 178 applicants, 30 students (16.8%) from 21 universities were selected to participate (50% female and 50% male students). All students completed both questionnaires. The overall rating was 9.47 on a 10-point scale. While the self-reported knowledge level increased from 6.47 (day 1) to 7.50 (day 3), almost all participants (96.7%, n = 29/30) mentioned an increased interest in the specialization of radiology after the event. Interestingly, most students (96.7%) preferred onsite teaching instead of online teaching and chose residents over board-certified radiologists as teachers.

Conclusion
Intensive three-day courses are valuable tools to strengthen interest in radiology and increase knowledge among medical students. Particularly, students who already have a tendency to specialize in radiology are further motivated.
Pathobionts from chemically disrupted gut microbiota induce insulin-dependent diabetes in mice
Authors: Yang Xin , Wang Zhiyi , Niu Junling , Zhai Rui , Xue Xinhe , Wu Guojun , Fang Yuanyuan , Meng Guangxun , Yuan Huijuan , Zhao Liping , Zhang Chenhong 
Published: March 2023

Abstract
Background
Dysbiotic gut microbiome, genetically predisposed or chemically disrupted, has been linked with insulin-dependent diabetes (IDD) including autoimmune type 1 diabetes (T1D) in both humans and animal models. However, specific IDD-inducing gut bacteria remain to be identified and their casual role in disease development demonstrated via experiments that can fulfill Koch’s postulates.

Results
Here, we show that novel gut pathobionts in the Muribaculaceae family, enriched by a low-dose dextran sulfate sodium (DSS) treatment, translocated to the pancreas and caused local inflammation, beta cell destruction and IDD in C57BL/6 mice. Antibiotic removal and transplantation of gut microbiota showed that this low DSS disrupted gut microbiota was both necessary and sufficient to induce IDD. Reduced butyrate content in the gut and decreased gene expression levels of an antimicrobial peptide in the pancreas allowed for the enrichment of selective members in the Muribaculaceae family in the gut and their translocation to the pancreas. Pure isolate of one such members induced IDD in wildtype germ-free mice on normal diet either alone or in combination with normal gut microbiome after gavaged into stomach and translocated to pancreas. Potential human relevance of this finding was shown by the induction of pancreatic inflammation, beta cell destruction and IDD development in antibiotic-treated wildtype mice via transplantation of gut microbiome from patients with IDD including autoimmune T1D.

Conclusion
The pathobionts that are chemically enriched in dysbiotic gut microbiota are sufficient to induce insulin-dependent diabetes after translocation to the pancreas. This indicates that IDD can be mainly a microbiome-dependent disease, inspiring the need to search for novel pathobionts for IDD development in humans.
Spatial distribution and tourism competition of intangible cultural heritage: take Guizhou, China as an example
Authors: Dong Baoling , Bai Kai , Sun Xiaolong , Wang Mengtao , Liu Yang 
Published: March 2023

Abstract
As a province inhabited by ethnic minorities in southwest China, Guizhou is rich in tourism and ICH (intangible cultural heritage) resources. The spatial distribution of ICH and the utilization of tourism and ICH resources in Guizhou worth studying, which is better for understanding the tourism competition situation and the tourism utilization of ICH. This research uses GIS tools for spatial analysis, mathematical formulas for calculating the abundance of ICH and tourism resources, as well as the matrix analysis for ICH and tourism competition, to identify the spatial distribution and tourism competition situation of ICH in Guizhou Province in 2019. The results show that: (1) in terms of the structural characteristics of the number and types of ICH in Guizhou Province, folk custom, traditional craftsmanship and traditional music have the highest number and proportion, while Quyi has the lowest. (2) Grade A scenic spots are mainly located in Zunyi City, while ICH scenic spots are mainly in Southeast Guizhou, Zunyi City, South Guizhou and Southwest Guizhou. Zunyi City ranks first in the abundance of tourism resources, and Liupanshui City ranks last. Southeast Guizhou has the highest ICH resources abundance, while Liupanshui City has the lowest. (3) Both the distribution of national-level and provincial-level ICH in Guizhou are aggregated. The national-level ICH are clustered in a large core area in geographical space, which is located in the southeast of Guizhou. The provincial-level ICH sites form a belt extending from southwest to southeast of the province, which is distributed at the junction of southeast of Guizhou, Guiyang and Anshun. (4) The provincial-level ICH in Guizhou has formed a belt-like high-density zone extending from southwest to southeast and three high-density core areas. (5) Through the analysis of a tourism competition matrix, we found that there are three types of development in nine cities in Guizhou Province. Zunyi City and Southeast Guizhou show the development characteristics of “tourism prosperity-ICH tourism prosperity”. Guiyang, Bijie, Anshun, Southwest Guizhou, Liupanshui and Tongren City show the development characteristics of “tourism depression-ICH tourism depression”. South Guizhou shows “tourism depression-ICH tourism prosperity”. These findings and methods will help cities determine their own strengths and weaknesses according to their resources, and narrow the regional development gap through formulating cultural and tourism development plans.
Morphological characterization of intraspecific variation for trichome traits in tomato (Solanum lycopersicum)
Authors: Kaur Satinderpal , Khanal Neetu , Dearth Robert , Kariyat Rupesh 
Published: March 2023

AbstractTrichomes, the hairlike protuberances in plants, have been well known to act as the first line of defense against herbivores, and abiotic stresses, along with other structural defenses such as spines, thorns, and waxes. We previously reported the tremendous variation in trichome traits among different wild and cultivated Solanum species and demonstrated that trichomes types and density are traditionally miscalculated and often misnamed. However, intraspecific variation in trichome traits is poorly understood, although this has implications for stress tolerance and resistance breeding programs in economically important crop species and can also mediate ecological interactions at multiple trophic levels in their wild congeners. In this study, using tomato as a model, we characterized the trichomes from 10 commonly grown varieties using a minimal sample prep desktop scanning electron microscopy, and followed up with estimating their dimensions across the varieties and trichome types. We hypothesized that although trichome number may vary, the varieties will have similar trichome types, based on current literature. Our results show that there is significant variation for trichome number as well as dimensions of trichome types among these varieties. Furthermore, when we separately analyzed the number and dimensions of commonly found glandular and non-glandular trichomes, the results were consistent with broad assessment of trichomes, showing consistent variation among varieties, suggesting that trichome studies should not be limited to basic classification into glandular and non-glandular, and should accommodate the sub-types and their dimensions.
Novel antibacterial and apatite forming restorative composite resin incorporated with hydrated calcium silicate
Authors: Yang Song-Yi , Han A Ruem , Choi Ji-Won , Kim Kwang-Mahn , Kwon Jae-Sung 
Published: March 2023

Abstract

Background
White Portland cement is a calcium silicate material. It exhibits antibacterial properties and is biocompatible. In addition, calcium silicate-based materials are known to release calcium ions and form apatite. The purpose of this study was to develop a novel bioactive restorative resin composite with antibacterial and apatite forming properties to prevent tooth caries at the interface of teeth and restorative materials, by incorporation of hydrated calcium silicate (hCS) derived from white Portland cement.

Methods
To prepare the experimental composite resins, a 30 wt% light-curable resin matrix and 70 wt% filler, which was mixed with hCS and silanized glass powder were prepared in following concentrations: 0, 17.5, 35.0, and 52.5 wt% hCS filler. The depth of cure, flexural strength, water sorption, solubility, and antibacterial effect were tested. After immersion in artificial saliva solution for 15, 30, 60, and 90 days, ion concentration by ICP-MS and apatite formation using SEM-EDS, Raman spectroscopy and XRD from experimental specimens were analyzed.

Results
All experimental groups showed clinically acceptable depths of cure and flexural strength for the use as the restorative composite resin. Water sorption, solubility, released Ca and Si ions increased with the addition of hCS to the experimental composite resin. Experimental groups containing hCS showed greater antibacterial effects compared with the 0 wt% hCS filler group (p < 0.05). The 52.5 wt% hCS filler group produced precipitates mainly composed of Ca and P detected as hydroxyapatite after immersion in artificial saliva solution for 30, 60, and 90 days.

Conclusions
This results show that composite resins containing hCS filler is effective in antibacterial effects. hCS has also apatite formation ability for reducing gap size of microleakage by accumulating hydroxyapatite precipitates at the restoration-tooth interface. Therefore, novel composite resin containing hCS is promising bioactive resin because of its clinically acceptable physiochemical properties, antibacterial properties, and self-sealing potential for prevention of microleakage for longer usage of restorations.
Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc
Authors: Koo Young Won , Lim Chang Su , Darai Anjani , Lee JiUn , Kim Wonjin , Han Inbo , Kim Geun Hyung 
Published: March 2023

Abstract

Background
Intervertebral disc degeneration (IVDD) is a common cause of chronic low back pain (LBP) and a socioeconomic burden worldwide. Conservative therapies and surgical treatments provide only symptomatic pain relief without promoting intervertebral disc (IVD) regeneration. Therefore, the clinical demand for disc regenerative therapies for disc repair is high.

Methods
In this study, we used a rat tail nucleotomy model to develop mechanically stable collagen-cryogel and fibrillated collagen with shape-memory for use in minimally invasive surgery for effective treatment of IVDD. The collagen was loaded with hyaluronic acid (HA) into a rat tail nucleotomy model.

Results
The shape-memory collagen structures exhibited outstanding chondrogenic activities, having completely similar physical properties to those of a typical shape-memory alginate construct in terms of water absorption, compressive properties, and shape-memorability behavior. The treatment of rat tail nucleotomy model with shape-memory collagen-cryogel/HA alleviated mechanical allodynia, maintained a higher concentration of water content, and preserved the disc structure by restoring the matrix proteins.

Conclusion
According to these results, the collagen-based structure could effectively repair and maintain the IVD matrix better than the controls, including HA only and shape-memory alginate with HA.
Interdependency patterns of potential seaport risk factors in relation to supply chain disruption in Indonesia
Authors: Do. Bagus Muhammad Reza , Hanaoka Shinya 
Published: March 2023

AbstractAs an integral part of the global supply chain network, Indonesian supply chain entities should understand conditional seaport risk factors that could lead to seaport threats that affect supply chain continuity. This study aims to provide a procedure for evaluating the interdependencies, implications, and correlations among various seaport risk factors for supply chain threats, specifically by investigating current practices in the developed economic region of Indonesian seaport operations. The study uses a rough set method to solve feature selection problems and multivariate analysis of variance to assess the correlation between dependent and independent variables. We find 39 conditional seaport risk factors that are potentially influenced by about 21 dependent factors related to seaport-fulcrum supply chain entities. Furthermore, threats from the planning process, infrastructure, seaport service process, distribution process, financial costs of nuclear enterprises and security existed and affluent highest potential risk in Indonesia.
Stoss therapy versus weekly regimen of vitamin D in children with chronic liver disease: a randomized pilot study
Authors: Abouzeed Hebatallah Saad , Bahey Eldin Lerine , El Masry Shereen Mohamed , Naguib Gina Gamal , Nagy Mona Ali , Toaima Nadin Nabil , Abdel-Ghaffar Tawhida Yassin 
Published: March 2023

Abstract

Background
Vitamin D, a hormone involved in the regulation of mineral homeostasis, protects skeletal integrity and modulates cell growth and differentiation. Recently, its potential antifibrotic effects have also been identified. Children with chronic liver disease mostly suffer from vitamin D deficiency. However, little knowledge is known regarding the optimum regimen that can be utilized effectively and safely to correct vitamin D deficiency in these patients and whether it could be effective in reversal or at least halting the progressive process of liver fibrosis. This study is conducted to answer these questions.

Results
Twenty-four children with chronic liver disease (13 boys and 11 girls) were included in the study. Their age ranged from 4.5 to 11.5 years with median age of 8 years. The aetiology of liver disease was heterogenous with autoimmune hepatitis, glycogen storage disease, or chronic hepatitis, and hepatitis C affects the majority. The patients were divided into two matched groups: group A (n:12) that received stoss parenteral intramuscular vitamin D3 (cholecalciferol) therapy (200,000 IU) once followed by 600 IU/day orally for 6 months (this is equivalent to the RDA as maintenance therapy) and group B (n:12) that received 50,000 IU/week oral vitamin D3 (cholecalciferol) therapy in divided daily doses adding on the maintenance dose 600 IU/day for the first 4 weeks followed by only 600 IU/day orally for the rest of the 6 months (5 months). Following vitamin D3 supplementation, in group A (vitamin D stoss therapy group) and group B (vitamin D oral therapy group), there were statistically significant improvement of Ca, alkaline phosphatase, and vitamin D levels, though there was no difference in between both groups. No significant correlation could be found between vitamin D changes and fibroscan changes in either group.

Conclusion
Vitamin D therapy using stoss dose followed by oral therapy or oral vitamin D therapy from the start was equally safe and effective in improving the clinical and laboratory metabolic bone profile abnormalities. Vitamin D effect on liver fibrosis progression or reversion in children is still not understood, and further studies are needed in this field taking in consideration the various causes of liver disease in children.