bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laboratory methods for monitoring resistance to methycillin of Staphylococcus of zoonotic origin
Authors: Makavchik S. A. , Borisova M. S. 
Published: April 2023

The development of antibiotic resistance in microorganisms is considered a modern phenomenon, which is usually caused by empirical antibiotic therapy. One of the representatives with high antibiotic resistance is staphylococcus aureus.Staphylococcus aureus is a pathogen resistant to broad-spectrum antibiotics and is responsible for most nosocomial infections. This microorganism synthesizes penicillinase, an enzyme that breaks down penicillin molecules. To effectively combat Staphylococcus aureus, a modified penicillin, methicillin, was previously used, the molecule of which was not destroyed by this enzyme. However, methicillin-resistant strains of this pathogen are also known today.As part of the group of methicillin-resistant staphylococci (MRSA), there are three subgroups: nosocomial variants of MRSA, community-acquired MRSA, MRSA associated with farm animals, common among farm animals (livestockassociated MRSA, LA-MRSA). MRSA are characterized by multidrug resistance.It was previously believed that Staphylococcus intermedius is the most dangerous staphylococcus for pets. However, over the past few years it has been found that the most common is Staphylococcus pseudintermedius, which is gaining increasing relevance. Staphylococcus pseudintermedius (MRSP) is a bacterium commonly found on the skin, mouth, nasal mucosa, and gastrointestinal tract in approximately 50% of pets. Unlike MRSA, MRSP is much more common in dogs and cats. One of the main methods of laboratory control over their spread is a monitoring study of the epizootic and epidemiological situation among animals and the population.The home environment plays a major role in the transmission of MRSA and MRSP, therefore, by taking careful measures to prevent the spread of infection, the likelihood of infection in both companion animals and humans can be reduced.The determination of sensitivity and resistance by phenotypic methods is carried out using: disk diffusion method, broth microdilution method, agar dilution method, E-test method.Laboratory methods for monitoring the mechanisms of resistance of staphylococci to methicillin include phenotypic methods using disks with oxacillin, or cefoxitin.Genotypic methods using polymerase chain reaction allow detecting the mecA, mecC gene.For a serological diagnostic method, a latex agglutination test is used to identify MRSA and MSSA (PSB2a / PSB2 spicelin-binding protein).Treatment of animals requires special attention because infections caused by multi-resistant staphylococci are more severe, chronic or latent, which increases the duration of treatment.
Specifics of the use of bovine blood serum in the technology of cultivation of avian metapneumovirus
Authors: Skorik A. S. , Pankratov S. V. , Kron N. V. 
Published: April 2023

When obtaining virus-containing material of avian metapneumovirus infection (MPVI) on Vero cell culture, special attention should be paid to the composition of growth and supporting nutrient media, since their components directly affect the proliferation and viability of the cell culture, and hence the quality of the resulting target product.For Vero cell culture, the main nutrient medium is the DMEM medium, into which various additional components are added. It is customary to add up to 10% of fetal bovine serum (FBS) to the growth medium, as for the supporting medium, the question of the need to add blood serum of cattle (cattle) to it does not have an unambiguous solution. Since the financial costs of nutrient media have a significant impact on the cost of the final product, it is necessary to analyze the economic feasibility of using one or another component, in connection with which we have studied the role of blood serum of cattle (FBS and blood serum of adult cattle) in the technology of obtaining the MPVI virus.As a result of the conducted studies, it was revealed that in the growth medium for Vero cell culture, it is possible to replace FBS with blood serum of adult cattle with no more than two consecutive passages. However, if the Vero cell culture is replanted more than twice, then such a replacement is impossible, since already in the 3-4 passage there are signs of a decrease in the rate of cell division and cell culture degeneration. A large number of voids and rounded cells are noted in the cell layer, which makes it impossible to use it further for the cultivation of viruses or further replanting.It was also found that when cultivating the MPVI virus on a Vero cell culture, the DMEM maintenance medium containing 2% of cattle blood serum is optimal for use, since it ensures the production of virus-containing material with higher infectious activity compared to the DMEM medium, which does not contain or contains 10% of cattle blood serum at all.
Use of sexed semen for reproduction in dairy cattle breeding
Authors: Paderina R. V. , Vinogradova N. D. 
Published: April 2023

Today, farms use traditional semen and sememen divided by sex for insemination. The use of sexed sperm and the increase in the number of heifers in the offspring due to this will allow breeding enterprises: to ensure optimal parameters for entering the first calves into their own herd; refuse to acquire expensive breeding young from other farms; significantly reduce the cost of raising gobies sold for meat; reduce the number of difficult calving and reduce culling of animals for gynecological diseases; improve the economic condition of the enterprise.The article provides the results of the use of sexed sperm in one of the farms of the Kirov region. As a result of the studies, it was found that the use of sexed semen makes it possible to significantly increase the number of calves in the obtained offspring by 2 times compared to the use of traditional seed 94.3% versus 48.2% of the use of traditional semen.The effectiveness of the use of sexed semen is significantly higher when used on heifers: an increase of 7.5% in the effectiveness of the first insemination; a 41.9% decrease in steely animal attrition and a 0.2% decrease in abortion rates.An essential condition for the successful use of sexed semen is the health of the herd, the qualification of an artificial insemination technician and a veterinarian.
Economic efficiency of castration of boars using different methods on the background of immunocorrection under the conditions of industrial technology
Authors: Reshetnyak V. V. , Stekolnikov A. A. , Burdeyny V. V. , Treskin M. S. 
Published: April 2023

The article presents the results of determining the economic efficiency of the use of two immunocorrectors — thymogen and mikovit (ribav) during castration of boars in industrial technology.The studies were performed on 49 boars (aged 5 months), divided into seven groups — two control and five experimental ones (n=7 in each).All animals were castrated: in the 1st control, 1st, 2nd, 3rd and 4th experimental groups — using open method; in the 2nd control and the 5th experimental groups — using closed method. Preoperative support was carried out according to the methodology used to the farm.The boars of the experimental groups were additionally treated with immunomodulators: in the first one — with thymogen (five-day course before surgery), in the second one — with mycovit (ribav), according to the scheme of the first group with local application after surgery, in the third — according to the scheme of the first group, but after the surgery; in the 4th and 5th — only with the working solution of mikovit (ribav) according to the method of the 2nd experimental group.The economic effect of the developed schemes was defined as the difference between the damage prevented as a result of veterinary measures and veterinary costs, taking into account the additional cost obtained by increasing the quantity of manufactured products and improving their quality. As an additional method, the total index of the effectiveness of veterinary measures was used, which was calculated by multiplying the economic damage by veterinary costs (per animal in each group) and then dividing the resulting number by their minimum multiplication.It was shown that the inclusion of thymogen at a dose of 100 μg/cm3 into the basic regimen of intramuscular injections with a five-day course after surgery using an open castration method or irrigation of the wound surface according to the above scheme with the solution of mycovit (ribav) can significantly increase the economic effect of veterinary measures. In the conditions of industrial pig breeding (taking into account the higher manufacturability of the application), preference should be given to thymogen.
Analysis of the pharmacokinetic properties of a native drug on the basis of a complex of sorbents
Authors: Popova O. S. 
Published: April 2023

Absolutely all preclinical studies involve three groups of experiments: preclinical studies of the pharmacokinetics of the substance, including toxicokinetics, preclinical studies of the pharmacokinetics of the drug and the clinical study of pharmacokinetics on target animals. Pharmacokinetic studies of native drugs are complex because they usually involve the administration of complex components. Thus, only the use of the whole complex of pharmacognostic, chemical, spectral, physicochemical and biological methods can provide an objective assessment of the authenticity and quality of medicinal herbal raw materials and medicinal herbal preparations. The study used Wistar white rats, weight 150-160g according to the Guidelines for Evaluation of Medicinal Products (3).The purpose of the study was to evaluate the pharmacokinetic properties of a new feed additive in the form of a premix of sorbents and phytocompositions: milk thistle (DV-silibinin, Pharmcenter VILAR CJSC), thyme (DV-timol, Akhma100). Supplements in the form of finished dosage forms were standardized for the active substance, and have a constant composition. Thus, it allowed to create a study design for a new substance in one animal species. Since the composition of the sorption component was studied earlier, the introduced substances were considered separately and in combination with sorbents.After a single injection, the linearity of the pharmacokinetics was assessed, based on the administered different doses, the analysis of the main biochemical parameters of the blood was carried out, and the bioavailability was also calculated. Thus, the new premix based on sorbents and plant substances in the form of milk thistle and thyme does not have a negative effect on the body of laboratory animals. As a result of the studies, LD50 and LD100 were not established for the drug, the drug is classified as low-toxic, belongs to the fourth hazard class according to GOST 12.1.007-76 "Harmful substances", which allows further toxico-therapeutic tests.
Fluxomics as a methodological tool for designing drugs
Authors: Ponamarev V. S. 
Published: April 2023

This article defines fluxomics as a biomedical science and an area of metabolic research, which is used as a methodological tool for the design of medicinal substances, including for veterinary use.The scientific novelty of the publication lies in the complexity of the ongoing review of existing research in the field of the latest methods of designing pharmaceutical substances (including those based on digital transformation), such as the study of cell flux, with a description of the main methodological approaches and principles. The main author's hypothesis of this study is the possibility of identifying the most promising approaches in terms of veterinary pharmacology for their further possible introduction into industry practice.The purpose of the review is to substantiate the priority use of the Fluxomics methodology as a stage in the development of new drugs for veterinary use with the study of the main methodological approaches.The information retrieval methodology was based on such general scientific methods of cognition as: a review of specialized search engines and databases of scientific and research data (Scopus, WoS, PubMed) over the past 10 years, of which the most informative ones were selected, analysis of the identified results, and their comparison by relevance.As a result of the analysis, the role of fluxomics in the structure of "ohmic" sciences was revealed, the main concepts that determine the development of fluxomics as a set of algorithms for the study of metabolic networks, analytical and instrumental methods for implementing fluxomic models were considered, the main problems and prospects for using this methodological apparatus in the context of designing and further validation of drug candidate substances.
Modern antihelminth medications for cattle (review)
Authors: Yaroshchuk A. I. 
Published: April 2023

The article contains an analysis of existing modern anthelmintic drugs for cattle. Trade names of drugs are divided into groups according to the active substances that make up their composition. The article discusses in detail the spectrum of action of the studied anthelmintic drugs on parasitic worms, and also displays special instructions from drug manufacturers for the use of milk and meat from animals that have been dewormed.Even the competent use of anthelmintic drugs will not help to achieve the desired economic results from the livestock complex if the veterinary rules for keeping and feeding animals are not observed at the production site, and it is also important to correctly plan and implement all necessary veterinary and sanitary measures.
PILOMATRIOXOMA EN UN PACIENTE CON VIH - PRESENTACION DE UN CASO INUSUAL
Authors: Rivera Ivan , Ramirez Marianela 
Published: April 2023

El pilomatrixoma es una tumuracion benigna poco frecuente piloso, es mas frecuente durante la infancia. La forma multiple de pilomatrixoma es rara y mas persinas con VIH, su presencia se asocia a sindromes sistemcios, entre los cuales destaca la distrodia miotonica. El diagnóstico definitivo de pilomatrixoma es histopatológico, aunque los estudios complementarios como la ecografía son útiles para confirmar el mismo. El tratamiento curativo es la resección quirúrgica de la lesión, con baja tasa de recurrencia.
Se presenta el caso clínico de un paciente de sexo masculino con VIH, de 50 años de edad, con diagnóstico de pilomatrixoma de características clínicas inusuales: forma múltiple y crecimiento rápido.
ESTILOS Y TÉCNICAS DE APRENDIZAJE UTILIZADAS POR ESTUDIANTES DE FISIOTERAPIA Y KINESIOLOGíA DE PRIMER SEMESTRE-GESTIÓN 2015 EN LA UNIVERSIDAD PRIVADA DEL VALLE
Authors: Guzmán Miranda Cinthia Verónica 
Published: April 2023

La visión deductiva del uso combinado de técnicas y estilos de aprendizaje nos lleva a determinar los que son utilizados por los estudiantes de primer semestre (gestión 2015) de la carrera de Fisioterapia y Kinesiología de la Universidad del Valle. Para ello se realizó un trabajo de Investigación de tipo prospectivo, transversal y analítico. El universo de estudio cuenta con 56 estudiantes de la Carrera de Fisioterapia del primer semestre de los periodos 1-11/2015.
El instrumento utilizado fue una encuesta preelaborada con fuentes de referencia que contenían preguntas abiertas y cerradas. Los datos emergentes fueron registrados en el paquete Excel v. 2013 para su tabulación y análisis respectivo. Los resultados encontrados indican que los estudiantes utilizan dos técnicas de estudio: la lectura y videos y audios; mientras que en cuanto a estilos de aprendizaje se refiere, predominan el estilo de aprendizaje visual, y kinestésico.
Estos resultados nos indican que el universo estudiado demuestra deficiencia con el uso de técnicas de estudio y la relación con los estilos de aprendizaje con los que cuenta cada estudiante. Después de realizar la encuesta, identificar las técnicas y estilos de aprendizaje se pudo determinar que los estudiantes de la carrera de Fisioterapia y Kinesiología del primer semestre necesitan, a partir del fortalecimiento de sus nociones, aplicar técnicas y estilos que se traducirán en la adquisición conceptual de teoría y práctica necesaria para obtener aprendizajes significativos.
CASO CLINICO: VARÓN DE 50 AÑOS CON PANICULITIS MESENTÉRICA
Authors: Mariño Ortiz Rodrigo S. , Peñaranda Molina Juan J. , Hernández Gaillard Lydia M. 
Published: April 2023

La paniculitis mesentérica es una enfermedad inflamatoria crónica, rara, con afección focal o multifocal del tejido adiposo del mesenterio intestinal con una demostrada predilección por el intestino delgado, de causa desconocida, de la cual se han descrito menos de 300 casos en la literatura médica hasta el momento.
En los casos descritos, se la asocia frecuentemente a otras enfermedades no transmisibles, como ser trastornos autoinmunitarios (vasculitis, artritis reumática, enfermedades granulomatosas), procesos neoplásicos e inflamatorios; lo cual puede orientarnos a proponer, como mecanismo patogénico, una respuesta autoinmune a fuentes desconocidas o una respuesta isquémica del mesenterio. La sintomatología provocada por esta enfermedad es de igual forma inespecífica, provocando dolor abdominal, astenia, alteraciones en el tránsito intestinal, pérdida de peso y otros.
Según estudios realizados, se aproxima que alrededor del 90% de los diagnósticos de esta enfermedad son incidentales al hacer estudios de TC en patologías no asociadas, o durante intervenciones quirúrgicas abdominales por laparoscopía o laparotomía. En nuestro centro fue diagnosticado, por histopatología, un varón de 50 años con un cuadro clínico compatible con el de una suboclusión intestinal alta y sospecha de linfoma intestinal. El paciente fue tratado exitosamente con administración de corticoides una vez realizado el diagnóstico.
INCIDENCIA DE PROTOZOOS INTESTINALES EN PACIENTES DEL SISTEMA INTEGRAL DE SALUD ATENDIDOS EN EL HOSPITAL "LA PAZ" EN EL SEGUNDO SEMESTRE DE LA GESTIÓN 2014
Authors: Oblitas Jerez Soledad del Carmen , Bravo Lecoña Gabriela 
Published: April 2023

El presente estudio ha determinado la incidencia de protozoos intestinales en pacientes del Sistema Integral de Salud atendidos en el Hospital La Paz en el segundo semestre de la gestión 2014. Bajo el siguiente método, el estudio es de carácter descriptivo, bajo un corte transeccional, donde el universo está determinado por los casos de estudio de Coproparasitológico simple, Coproparasitológico seriado y Moco fecal, realizados en el mismo hospital, además se efectuó una encuesta a cien pacientes entre niños, mujeres y ancianos que son parte del Sistema Integral de Salud.
Se cumplió un estudio estadístico de 491 casos, de los cuales 71 casos fueron positivos que representan 14,4% del total. El análisis mayormente llevado a cabo fue el Análisis de Moco Fecal, debido a que es el que permite dar un diagnóstico rápido, que en este caso se presentó en un total de 289 casos, representado más del 50% del total de análisis revisados. En esta dimensión, el estudio de Coproparasitológico Seriado, es el que mayores resultados positivos ha determinado, encontrando un total de 37 casos positivos que representan más del 7,5% de los casos analizados. Del total de casos positivos analizados, 36 de ellos mostraron la presencia de Entamoeba Coli, que representa el 7,4% del total de la población estudiada.
CONTROL DE PLACA PERIODONTAL EN PACIENTES CONCIENTIZADOS EN HIGIENE DENTAL EN LA CLÍNlCA ODONTOLÓGICA UNIVALLE GESTIÓN 2010 - 2011 LA SALUD
Authors: Delgadillo Trigo Martin 
Published: April 2023

Se ha determinado que una de las enfermedades que más prevalencia e incidencia tiene en la sociedad es la enfermedad periodontal. Se determinó mediante la aplicación del índice de O'Leary la cantidad de placa dental en el paciente.
Esta investigación se desarrolló en la Clínica Odontológica Univalle con el objetivo principal de determinar el control de placa periodontal en pacientes concientizados en higiene dental, para poder determinar si los métodos preventivos de enseñanza de aseo bucal y concientización que son utilizados, fueron eficaces en los pacientes tratados periodontalmente. Fue una investigación de tipo prospectivo, transversal y experimental. Esta información fue obtenida por medio de 50 historias clínicas de pacientes atendidos en la cátedra de Preventiva y Periodoncia durante la gestión 2010 - 2011, confeccionadas por los estudiantes de cuarto año de la Clínica Odontológica Univalle.
Los principales resultados fueron: el 90% de los pacientes utilizaban una técnica de cepillado combinada (horizontal, vertical y circular), con una frecuencia de cepillado de dos veces por día. La diferencia entre los que usan hilo dental y los que no Io usan es de 8 pacientes. Se observó una reducción promedio del índice de O'leary de 36,3% con la técnica combinada a 15,5% con la técnica de cepillado de Stillman, lo cual es compatible con salud bucal.
PRINCIPIOS FISICOS Y TERAPÉUTICOS DE LA HIDROCINESITERAPIA (TERAPIA ACUÁTICA)
Authors: Rivero Callejas Lenny Helen 
Published: April 2023

Dentro del proceso rehabilitador, la gravedad es un fenómeno físico que puede tornarse en un elemento que dificulta la progresión de algunas capacidades en el paciente, siendo éste un factor muy importante de fracaso durante la terapia física, debido a que el paciente siente gran frustración al no poder realizar con éxito los ejercicios solicitados.
La rehabilitación dentro el agua, gracias a sus diversas propiedades, promueve el éxito de la terapia para la recuperación funcional. Esta herramienta kinésica de primer orden nos da la posibilidad de acortar los plazos terapéuticos y de recuperar la independencia del paciente en las Actividades de la Vida Diaria (AVD), reinsertándolo a su medio social o laboral en un menor tiempo, recuperando así la autoestima e independencia del individuo.
BENEFICIOS DE LA ACELERACIÓN DE FLUJO ESPIRATORIO EN EL TRATAMIENTO DE ATELECTASIA OBSTRUCTIVA ASOCIADA A VENTILAClÓN MECÁNICA EN PACIENTES DE LA UNIDAD DE CUIDADOS INTENSIVOS NEONATALES
Authors: Vasquez Quisberth Minerva , Valenzuela Andia Abel 
Published: April 2023

La atelectasia es el colapso parcial o total del pulmón con disminución del volumen pulmonar generado por factores multietiológicos.
La ventilación mecánica es un procedimiento invasivo cuya aplicación implica en ocasiones severos riesgos que deben preverse y, en lo posible, deben ser identificados en forma temprana para implementar un tratamiento inmediato y efectivo.
El rol del kinesiólogo es de gran importancia en la resolución de complicaciones respiratorias, principalmente de la atelectasia, puesto que la rehabilitación respiratoria tiene como objetivo facilitar la eliminación de secreciones traqueobronquiales.
La técnica kinésica de vibraciones o aceleración de flujos espirados en el tórax es un procedimiento que se realiza durante la fase espiratoria, su frecuencia es de 5 a 25 Hz por segundo. Las vibraciones aplicadas en la pared torácicas de los neonatos deben ser muy cuidadosas y suaves