bluuebunny/crossref_metadata_2025_split huggingface dataset:
C-X-C motif chemokine ligand 12 improves the developmental potential of bovine oocytes by activating SH2 domain-containing tyrosine phosphatase 2 during maturation
Authors: Zhang Min , Zhang Jingcheng , Wang Debao , Liu Zhengqing , Xing Kangning , Wang Yongsheng , Jiao Mei , Wang Yong , Shi Binqiang , Zhang Hexu , Zhang Yong 
Published: July 2023

AbstractIn vitro maturation of mammalian oocytes is an important means in assisted reproductive technology. Most bovine immature oocytes complete nuclear maturation, but less than half develop to the blastocyst stage after fertilization. Thus, inefficient in vitro production is mainly caused by a suboptimal in vitro culture process, in which oocyte quality appears to be the limiting factor. In our study, a potential maternal regulator, C-X-C motif chemokine ligand 12, was identified by analyzing transcriptome data. C-X-C motif chemokine ligand 12 supplementation promoted the developmental potential of oocytes by improving protein synthesis and reorganizing cortical granules and mitochondria during in vitro maturation, which eventually increased blastocyst formation efficiency and cell number after parthenogenesis, fertilization, and cloning. All these promoting effects by C-X-C motif chemokine ligand 12 were achieved by activating SH2 domain-containing tyrosine phosphatase 2, thereby promoting the mitogen-activated protein kinase signaling pathway. These findings provide an in vitro maturation system that closely resembles the maternal environment to provide high-quality oocytes for in vitro production.
MTM1 overexpression prevents and reverts BIN1-related centronuclear myopathy
Authors: Giraud Quentin , Spiegelhalter Coralie , Messaddeq Nadia , Laporte Jocelyn 
Published: July 2023

Abstract
Centronuclear and myotubular myopathies (CNM) are rare and severe genetic diseases associated with muscle weakness and atrophy as well as intracellular disorganization of myofibres. The main mutated proteins control lipid and membrane dynamics and are the lipid phosphatase myotubularin (MTM1), and the membrane remodelling proteins amphiphysin 2 (BIN1) and dynamin 2 (DNM2). There is no available therapy. Here, to validate a novel therapeutic strategy for BIN1- and DNM2-CNM, we evaluated adeno-associated virus-mediated MTM1 (AAV-MTM1 ) overexpression in relevant mouse models.
Early systemic MTM1 overexpression prevented the development of the CNM pathology in Bin1mck−/− mice, while late intramuscular MTM1 expression partially reverted the established phenotypes after only 4 weeks of treatment. However, AAV-MTM1 injection did not change the DNM2-CNM mouse phenotypes. We investigated the mechanism of the rescue of the myopathy in BIN1-CNM and found that the lipid phosphatase activity of MTM1 was essential for the rescue of muscle atrophy and myofibre hypotrophy but dispensable for the rescue of myofibre disorganization including organelle mis-position and T-tubule defects. Furthermore, the improvement of T-tubule organization correlated with normalization of key regulators of T-tubule morphogenesis, dysferlin and caveolin.
Overall, these data support the inclusion of BIN1-CNM patients in an AAV-MTM1 clinical trial.
What Influences Patients' Adherence to Healthcare Worker Prescription in Primary Healthcare Facilities in Burkina Faso? A Qualitative Account of Barriers and Facilitators
Authors: Compaoré Adélaïde , Nikièma Jacqueline , Kiemdé Francois , Tinto Halidou , Salami Olawale , Nkeramahame Juvenal , Olliaro Piero , Horgan Philip 
Published: July 2023

Abstract

Background
This study explores the factors influencing patients and caregivers' adherence to prescription of healthcare workers (HCWs).

Methods
The study was conducted in Temnaore and Pella, in the Nanoro health district in Burkina Faso. HCWs and community members were purposively recruited from 4 communities seeking care at the selected primary healthcare facilities for the clinical trial to attend in-depth interviews and focus group discussions on the factors influencing adherence to prescription. The Behaviour Change Wheel incorporating the Capability, Opportunity, and Motivation Behaviour approach was used.

Results
Factors influencing the ability of patients to obtain the prescribed medicine include the availability of medicines and money and the perception of consequences for not getting the medicine. Regarding compliance with the intake of medicines, communication was considered a key factor whose effectiveness depends on the performance of HCWs and on the attention of patients. It is followed by other factors such as adequate management of patients, social influences, the patient’s beliefs regarding treatment, and memory.

Conclusions
This research highlights factors influencing adherence to HCWs’ prescription from the perspective of the community members and HCWs and therefore provides contextual enablers and barriers, which allows for the development of an intervention to support the clinical trial.
The Impact of Crohn’s Perianal Fistula on Quality of Life: Results of an International Patient Survey
Authors: Spinelli Antonino , Yanai Henit , Girardi Paolo , Milicevic Slobodan , Carvello Michele , Maroli Annalisa , Avedano Luisa 
Published: July 2023

Abstract

Background
Crohn’s perianal fistula is a disabling manifestation of Crohn’s disease. However, the additional burden of perianal fistula on patients with only Crohn’s disease remains to be addressed. This patient-reported survey considered outcomes of two domains: “diagnosis” (eg, symptoms) and “living with the disease” (eg, quality of life, well-being, and relationships).

Methods
Patients with perianal fistula and Crohn’s disease completed an online, self-selective, anonymous, 46-item survey available in 11 languages hosted on the European Federation of Crohn’s & Ulcerative Colitis Associations and national patient association websites. The survey was conducted between July and December 2019 in Europe and other regions. Likert scales and closed questions were used to assess outcomes.

Results
Of the 820 respondents with Crohn’s disease (67.2% women; median age, 40.0 years), 532 (64.9%) reported the presence of perianal fistula. Patients with perianal fistula reported a greater impact on overall quality of life (P &lt; .001), well-being (P &lt; .001), relationships (P &lt; .001), social life (P = .001), and work life (P = .012) than patients with only Crohn’s disease.

Conclusions
Perianal fistulas impact several domains of the life of patients with Crohn’s disease. These results may help healthcare practitioners plan therapeutic strategies that address the symptomatic and psychological burden experienced by patients with perianal fistulizing Crohn’s disease.
Heart age gap estimated by explainable advanced electrocardiography is associated with cardiovascular risk factors and survival
Authors: Lindow Thomas , Maanja Maren , Schelbert Erik B , Ribeiro Antônio H , Ribeiro Antonio Luiz P , Schlegel Todd T , Ugander Martin 
Published: July 2023

Abstract

Aims
Deep neural network artificial intelligence (DNN-AI)–based Heart Age estimations have been presented and used to show that the difference between an electrocardiogram (ECG)-estimated Heart Age and chronological age is associated with prognosis. An accurate ECG Heart Age, without DNNs, has been developed using explainable advanced ECG (A-ECG) methods. We aimed to evaluate the prognostic value of the explainable A-ECG Heart Age and compare its performance to a DNN-AI Heart Age.

Methods and results
Both A-ECG and DNN-AI Heart Age were applied to patients who had undergone clinical cardiovascular magnetic resonance imaging. The association between A-ECG or DNN-AI Heart Age Gap and cardiovascular risk factors was evaluated using logistic regression. The association between Heart Age Gaps and death or heart failure (HF) hospitalization was evaluated using Cox regression adjusted for clinical covariates/comorbidities. Among patients [n = 731, 103 (14.1%) deaths, 52 (7.1%) HF hospitalizations, median (interquartile range) follow-up 5.7 (4.7–6.7) years], A-ECG Heart Age Gap was associated with risk factors and outcomes [unadjusted hazard ratio (HR) (95% confidence interval) (5 year increments): 1.23 (1.13–1.34) and adjusted HR 1.11 (1.01–1.22)]. DNN-AI Heart Age Gap was associated with risk factors and outcomes after adjustments [HR (5 year increments): 1.11 (1.01–1.21)], but not in unadjusted analyses [HR 1.00 (0.93–1.08)], making it less easily applicable in clinical practice.

Conclusion
A-ECG Heart Age Gap is associated with cardiovascular risk factors and HF hospitalization or death. Explainable A-ECG Heart Age Gap has the potential for improving clinical adoption and prognostic performance compared with existing DNN-AI-type methods.
Decision Times Reveal Private Information in Strategic Settings: Evidence from Bargaining Experiments
Authors: Konovalov Arkady , Krajbich Ian 
Published: July 2023

Abstract
People respond quickly when they have a clear preference and slowly when they are close to indifference. The question is whether others exploit this tendency to infer private information. In two-stage bargaining experiments, we observe that the speed with which buyers reject sellers’ offers decreases with the size of the foregone surplus. This should allow sellers to infer buyers’ values from response times, creating an incentive for buyers to manipulate their response times. We experimentally identify distinct conditions under which subjects do, and do not, exhibit such strategic behaviour. These results provide the first insight into the possible use of response time as a strategic variable.
Acute and mid-term outcomes of ablation for atrial fibrillation with VISITAG SURPOINT: the Japan MIYABI registry
Authors: Okumura Ken , Inoue Koichi , Goya Masahiko , Origasa Hideki , Yamazaki Makiho , Nogami Akihiko 
Published: July 2023

Abstract

Aims
The effectiveness of pulmonary vein isolation (PVI) guided by VISITAG SURPOINT (VS) has been demonstrated in Western populations. However, data for Asian populations are limited. VS settings may differ for Asians, given their smaller body size. This study aimed to describe outcomes of radiofrequency atrial fibrillation (AF) ablation guided by VS in a large Asian population.

Methods and results
The prospective, observational, multicentre MIYABI registry collected real-world data from patients undergoing VS-guided AF ablation using ThermoCool SmartTouch and ThermoCool SmartTouch SF catheters from 50 Japanese centres. All patients had paroxysmal AF or persistent AF for &lt;6 months. Primary adverse events (PAEs) were evaluated for safety. The primary efficacy endpoint was the proportion of patients with PVI at the end of the procedure. Mid-term effectiveness (up to 12 months) was evaluated by freedom from documented atrial arrhythmias. Of the 1011 patients enrolled, 1002 completed AF ablation. The mean number of VS values per procedure was 428.8 on the anterior wall and 400.4 on the posterior wall. Nine patients (0.9%) experienced PAEs. Upon procedure completion, 99.7% of patients had PVI. Twelve-month freedom from atrial arrhythmia recurrence was 88.5%; 5.7% of patients were re-ablated. At repeat ablation, 54% of RSPV, 73% of RIPV, 70% of LSPV, and 86% of LIPV evaluated remained durably isolated.

Conclusion
Despite lower anterior wall VS values compared with the CLOSE protocol (≥550), the present study demonstrated comparable efficacy outcomes, indicating that a VS of ≥550 for the anterior wall may not be necessary for Asian patients.
Early detection of neurodevelopmental disorders in paediatric primary care: A scoping review
Authors: O’Hara Paulette T , Talero Cabrejo Pamela , Earland Tracey V 
Published: July 2023

Abstract

Background
Earlier detection of children at risk for neurodevelopmental disorders is critical and has longstanding repercussions if not addressed early enough.

Objectives
To explore the supporting or facilitating characteristics of paediatric primary care models of care for early detection in infants and toddlers at risk for neurodevelopmental disorders, identify practitioners involved, and describe how they align with occupational therapy’s scope of practice.

Methods
A scoping review following the Joanna Briggs Institute framework was used. PubMed Central, Cumulative Index to Nursing & Allied Health Literature, and Scopus databases were searched. The search was conducted between January and February 2022. Inclusion criteria were: children aged 0–3 years old; neurodevelopmental disorders including cerebral palsy (CP) and autism spectrum disorder (ASD); models of care used in the paediatric primary care setting and addressing concepts of timing and plasticity; peer-reviewed literature written in English; published between 2010 and 2022. Study protocol registered at https://doi.org/10.17605/OSF.IO/MD4K5

Results
We identified 1,434 publications, yielding 22 studies that met inclusion criteria. Models of care characteristics included the use of technology, education to parents and staff, funding to utilize innovative models of care, assessment variability, organizational management changes, increased visit length, earlier timeline for neurodevelopmental screening, and collaboration with current office staff or nonphysician practitioners. The top 4 providers were paediatricians, general or family practitioners, nurse/nurse practitioners, and office staff. All studies aligned with occupational therapy health promotion scope of practice and intervention approach yet did not include occupational therapy within the paediatric primary care setting.

Conclusions
No studies included occupational therapy as a healthcare provider that could be used within the paediatric primary care setting. However, all studies demonstrated models of care facilitating characteristics aligning with occupational therapy practice. Models of care facilitating characteristics identified interdisciplinary staff as a major contributor, which can include occupational therapy, to improve early detection within paediatric primary care.
Exploring mechanisms and contexts in a Peer Education Project to improve mental health literacy in schools in England: a qualitative realist evaluation
Authors: Curtin E L , Widnall E , Dodd S , Limmer M , Simmonds R , Russell A E , Kaley A , Kidger J 
Published: July 2023

Abstract
Poor adolescent mental health calls for universal prevention. The Mental Health Foundation’s ‘Peer Education Project’ equips older students (‘peer educators’) to teach younger students (‘peer learners’) about mental health. The peer-led lessons cover defining good and bad mental health, risk and protective factors, self-care, help-seeking and looking after one another. While previous pre-post evaluations have suggested effectiveness, the mechanisms through which the intervention improves mental health literacy remain unclear. We purposively recruited seven secondary schools across England from 2020 to 2022 and collected data through five observations, 12 staff interviews and 15 student focus groups (totalling 134 students; 46 peer educators aged 14–18 years and 88 peer learners aged 11–13 years). Our realist analysis adopted retroductive logic, intertwining deductive and inductive approaches to test the initial programme theory against insights arising from the data. We developed Context–Mechanisms–Outcome configurations related to four themes: (i) modelling behaviours and forming supportive relationships, (ii) relevant and appropriate content, (iii) peer educators feeling empowered and (iV) a school culture that prioritises mental health support. Our refined programme theory highlights key mechanisms, contexts conducive to achieving the outcomes and ways to improve training, recruitment and delivery to maximise effectiveness for similar peer-led initiatives.
Evaluation of the Live-Attenuated Intranasal Respiratory Syncytial Virus (RSV) Vaccine RSV/6120/ΔNS2/1030s in RSV-Seronegative Young Children
Authors: Karron Ruth A , Luongo Cindy , Woods Suzanne , Oliva Jennifer , Collins Peter L , Buchholz Ursula J ,   the RSVPed Team, Council-Dibitetto Christine , Gatto Milena , Ghasri Tina , Gormley Amanda , Herbert Kristi , Jordan Maria , Loehr Karen , Morsell Jason , Mateo Jocelyn San , Schappell Elizabeth , Smith Khadija , Soro Paula , Wanionek Kimberli , Weadon Cathleen 
Published: July 2023

Abstract

Background
Respiratory syncytial virus (RSV) is the leading cause of pediatric lower respiratory illness (LRI) and a vaccine for immunization of children is needed. RSV/6120/ΔNS2/1030s is a cDNA-derived live-vaccine candidate attenuated by deletion of the interferon antagonist NS2 gene and the genetically stabilized 1030s missense polymerase mutation in the polymerase, conferring temperature sensitivity.

Methods
A single intranasal dose of RSV/6120/ΔNS2/1030s was evaluated in a double-blind, placebo-controlled trial (vaccine to placebo ratio, 2:1) at 105.7 plaque-forming units (PFU) in 15 RSV-seropositive 12- to 59-month-old children, and at 105 PFU in 30 RSV-seronegative 6- to 24-month-old children.

Results
RSV/6120/ΔNS2/1030s infected 100% of RSV-seronegative vaccinees and was immunogenic (geometric mean RSV plaque-reduction neutralizing antibody titer [RSV-PRNT], 1:91) and genetically stable. Mild rhinorrhea was detected more frequently in vaccinees (18/20 vaccinees vs 4/10 placebo recipients, P = .007), and LRI occurred in 1 vaccinee during a period when only vaccine virus was detected. Following the RSV season, 5 of 16 vaccinees had ≥4-fold rises in RSV-PRNT with significantly higher titers than 4 of 10 placebo recipients with rises (1:1992 vs 1:274, P = .02). Thus, RSV/6120/ΔNS2/1030s primed for substantial anamnestic neutralizing antibody responses following naturally acquired RSV infection.

Conclusions
RSV/6120/ΔNS2/1030s is immunogenic and genetically stable in RSV-seronegative children, but the frequency of rhinorrhea in vaccinees exceeded that in placebo recipients.

Clinical Trials Registration
NCT03387137.
Absence of Proviral Human Immunodeficiency Virus (HIV) Type 1 Evolution in Early-Treated Individuals With HIV Switching to Dolutegravir Monotherapy During 48 Weeks
Authors: Jörimann Lisa , Tschumi Jasmin , Zeeb Marius , Leemann Christine , Schenkel Corinne D , Neumann Kathrin , Chaudron Sandra E , Zaheri Maryam , Frischknecht Paul , Neuner-Jehle Nadia , Kuster Herbert , Braun Dominique L , Grube Christina , Kouyos Roger , Metzner Karin J , Günthard Huldrych F ,   for the Swiss HIV Cohort Study (SHCS)
Published: July 2023

Abstract
Human immunodeficiency virus type 1 (HIV-1) infection is treated with antiretroviral therapy (ART), usually consisting of 2–3 different drugs, referred to as combination ART (cART). Our recent randomized clinical trial comparing a switch to dolutegravir monotherapy with continuation of cART in early-treated individuals demonstrated sustained virological suppression over 48 weeks. Here, we characterize the longitudinal landscape of the HIV-1 reservoir in these participants, with particular attention to potential differences between treatment groups regarding evidence of evolution as a proxy for low-level replication. Near full-length HIV-1 proviral polymerase chain reaction and next-generation sequencing was applied to longitudinal peripheral blood mononuclear cell samples to assess proviral evolution and the potential emergence of drug resistance mutations (DRMs). Neither an increase in genetic distance nor diversity over time was detected in participants of both treatment groups. Single proviral analysis showed high proportions of defective proviruses and low DRM numbers. No evidence for evolution during dolutegravir monotherapy was found in these early-treated individuals.
A Green-and-White Integrative Analytical Strategy Combining Univariate and Chemometric Techniques for Quantifying Recently Approved Multi-Drug Eye Solution and Potentially Cancer-Causing Impurities: Application to the Aqueous Humor
Authors: Attia Khalid A M , El-Olemy Ahmed , Eid Sherif M , Abbas Ahmed Emad F 
Published: July 2023

Abstract

Background
Drug impurities are now seen as a major threat to the production of pharmaceuticals around the world and a major part of the global contamination problem, especially when it comes to carcinogenic impurities.

Objective
We present the first spectrophotometric strategy based on a combination of univariate and multivariate methods as impurity profiling methods for the estimation of lignocaine (LIG) and fluorescein (FLS) with their carcinogenic impurities: 2,6-xylidine (XYL) and benzene-1,3-diol (BZD).

Method
The data processing strategy depends on overcoming unresolved bands by employing five affordable, accurate, selective, and sensitive methods. The methods applied were a direct UV univariate spectrophotometric analysis (D0) and four multivariate chemometric methods, including classical least squares (CLS), principal component regression (PCR), partial least squares (PLS), and genetic algorithm (GA-PLS). FLS analysis (1–16 μg/mL) was performed using the D0 method at 478 nm; then, the application of the ratio subtraction method (RSM) allowed the removal of interference caused by the FLS spectrum. From the resulting ratio spectra, LIG, XYL, and BZD can be efficiently determined by chemometrics. The calibration set was carefully selected at five concentration levels using a partial factorial training design, resulting in 25 mixtures with central levels of 160, 40, and 3 μg/mL for LIG, XYL, and BZD, respectively. Another 13 samples were applied to validate the predictive ability.

Results
The statistical parameters demonstrated exceptional recoveries and smaller prediction errors, confirming the experimental model’s predictive power.

Conclusions
The proposed approach was effectively tested using newly FDA-approved LIG and FLS pharmaceutical preparation and aqueous humor. Additionally, it was effectively assessed for whiteness, greenness, and sustainability using five assessment tools.

Highlights
With its remarkable analytical performance, sustainability, affordability, simplicity, and cost-efficiency, the proposed strategy is an indispensable tool for quality control and in situ analysis in little-equipped laboratories, increasing the proposed approach’s surveillance ability.
Estimation of population size with heterogeneous catchability and behavioural dependence: applications to air- and water-borne disease surveillance
Authors: Bhuyan Prajamitra , Chatterjee Kiranmoy 
Published: July 2023

AbstractPopulation size estimation based on the capture–recapture experiment is an interesting problem in various fields including epidemiology, criminology, demography, etc. In many real-life scenarios, there exists inherent heterogeneity among the individuals and dependency between capture and recapture attempts. A novel trivariate Bernoulli model is considered to incorporate these features, and the Bayesian estimation of the model parameters is suggested using data augmentation. Simulation results show robustness under model misspecification and the superiority of the performance of the proposed method over existing competitors. The method is applied to analyse real case studies on epidemiological surveillance. The results provide interesting insight on the heterogeneity and dependence involved in the capture–recapture mechanism. The methodology proposed can assist in effective decision-making and policy formulation.