bluuebunny/crossref_metadata_2025_split huggingface dataset:
METTL3‐Mediated m6A Methylation Stabilizes IFI27 to Drive Esophageal Squamous Cell Carcinoma Progression Through an IGF2BP2‐Dependent Mechanism
Authors: Zhang Xinhua , Bai Yu , Shang Linlin , Wang Yinghao , Yao Wenjian , Wu Sen 
Published: February 2025

ABSTRACTDysregulation of m6A modification has emerged as a vital factor in the development of esophageal squamous cell carcinoma (ESCC). Here, we sought to explore the critical role of m6A methylation mediated by the m6A methyltransferase METTL3 in ESCC. Protein expression analysis was performed by immunohistochemistry and immunoblot assays. The mRNA levels of METTL3 and IFI27 were detected by quantitative PCR. Cell sphere formation potential, migration, invasiveness, apoptosis, proliferation and viability were assessed by standard sphere formation, wound healing, transwell, flow cytometry, EdU and CCK‐8 assays, respectively. The impact of METTL3 or IGF2BP2 on IFI27 mRNA was evaluated by methylated RNA immunoprecipitation (MeRIP), RIP or mRNA stability analysis. Xenograft assays were used to detect the in vivo function of METTL3. Elevated levels of METTL3 were observed in ESCC tumors and cells, and these increased levels were associated with the declined prognosis of ESCC. MELLT3 depletion impeded ESCC cell growth, invasiveness, migration, and sphere formation, and induced cell apoptosis in vitro. Elevated IFI27 expression was positively correlated with METTL3 levels in ESCC. Moreover, METTL3 mediated m6A methylation of IFI27 mRNA to stabilize the mRNA. The m6A reader IGF2BP2 also affected m6A methylation and expression of IFI27 mRNA. Additionally, IFI27 re‐expression had a counteracting impact on the effects of METTL3 deficiency on in vitro ESCC cell behaviors and in vivo KYSE30 xenograft growth. Our findings demonstrate that METTL3‐mediated IFI27 mRNA m6A methylation drives ESCC development through an IGF2BP2‐dependent mechanism. Blocking the METTL3/IFI27 axis may be effective for preventing ESCC.
Unveiling Novel Targets in Lung Tumors for Enhanced Radiotherapy Efficacy: A Comprehensive Review
Authors: Muhammad Faris Anad , Adhab Ayat Hussein , Mahdi Morug Salih , Jain Vicky , Ganesan Subbulakshmi , Bhanot Deepak , Naidu K. Satyam , Kaur Sharnjeet , Mansoor Aseel Salah , Radi Usama Kadem , Abd Nasr Saadoun , Kariem Muthena 
Published: February 2025

ABSTRACTRadiotherapy is a cornerstone of lung cancer management, though its efficacy is frequently undermined by intrinsic and acquired radioresistance. This review examines the complexity of lung tumors, highlighting their potential as a reservoir of novel targets for radiosensitization. Ionizing radiation (IR) primarily exerts its effects through oxidative damage and DNA double‐strand breaks (DSBs). Lung cancer cells, however, develop mutations that enhance DNA damage response (DDR) and suppress cell death pathways. Additionally, interactions between tumor cells and tumor microenvironment (TME) components—including immune cells, stromal cells, and molecular mediators such as cytokines, chemokines, and growth factors—contribute to resistance against IR. Understanding these intricate relationships reveals potential targets to improve radiotherapy outcomes. Promising targets include DDR pathways, immunosuppressive cells and molecules, hypoxia, proangiogenic mediators, and other key signaling pathways. This review discusses emerging strategies, such as combining radiotherapy with immunomodulators, hypoxia and proangiogenic inhibitors, DDR‐targeting agents, and other innovative approaches. By offering a comprehensive analysis of the lung TME, this review underscores opportunities to enhance radiotherapy effectiveness through targeted radiosensitization strategies.
Mechanism of the microRNA‐373‐3p/LATS2 Axis in the Prognosis and Metastasis of Thyroid Cancer Patients
Authors: Gu Yingchao , Liu Hongbing , Shi Ming , Pu Fei 
Published: February 2025

ABSTRACTThis study focused on the role of the microRNA (miR)‐373‐3p/LATS2 axis in the prognosis and metastasis of thyroid cancer patients. miR‐373‐3p and LATS2 expression were assessed in thyroid cancer tissues and cells. The relationship between miR‐373‐3p and clinicopathological characteristics of patients with thyroid cancer and the impact of miR‐373‐3p and LATS2 expression levels on the survival and prognosis of thyroid cancer patients were analyzed. The targeting relationship between miR‐373‐3p and LATS2 was predicted and verified, and their impact on the malignant cell phenotype was assessed. Compared with adjacent normal tissues and normal human thyroid cells, miR‐373‐3p was highly expressed, while LATS2 was expressed at low levels in thyroid cancer tissues and cells (both p < 0.001). miR‐373‐3p expression was independent of age (p = 0.201) and gender (p = 0.516), and it was correlated with lymph node metastasis and TNM stage of thyroid cancer (both p < 0.001). Moreover, high miR‐373‐3p expression was associated with poor patient prognosis (p = 0.034). Interference with miR‐373‐3p or overexpression of LATS2 repressed KMH‐2 cell malignant phenotypes (all p < 0.05). miR‐373‐3p targeted and suppressed LATS2 expression. Interference with miR‐373‐3p blocked its inhibition on LATS2, thereby repressing thyroid cancer progression and metastasis.
The Impact of P‐Glycoprotein on CNS Drug Efflux and Variability in Response
Authors: Paul Priyanka R. , Mishra Manish K. , Bora Shivangi , Kukal Samiksha , Singh Anju , Kukreti Shrikant , Kukreti Ritushree 
Published: February 2025

ABSTRACTResistance against CNS drugs may arise from various mechanisms, with limited drug penetration across the blood‐brain barrier (BBB) being a significant contributing factor. The BBB employs efflux transporters like P‐glycoprotein (P‐gp) to safeguard the brain by removing toxins and xenobiotics, however, P‐gp also pumps out therapeutic drugs, and its upregulation in disease states can contribute to variability in drug response. While inhibiting P‐gp to prevent drug efflux seems appealing, it could lead to toxicity since P‐gp is also important for expulsion of toxins from the brain. This necessitates the incorporation of P‐gp substrate liability assessment into early drug discovery stages using appropriate experimental approaches. Therefore, this review aims to draw interest in this crucial area by analyzing the existing research on P‐gp's impact on brain distribution of major CNS drugs and exploring the detection methods for identifying P‐gp substrates. By identifying confirmed P‐gp substrates and evaluating effective detection methods, this work emphasizes the continued importance of monitoring P‐gp‐mediated CNS drug efflux out of the brain tissue. This knowledge can empower clinicians to anticipate potential treatment inefficacy and guide therapeutic decision‐making, ultimately leading to improved patient treatment outcomes.
Trophic Factors in Muscle‐Nerve Cross‐Talk Signaling Augment Muscle Fiber and Motor Endplate Development
Authors: Redden James T. , Kothe Sophie , Cohen David J. , Schwartz Zvi , McClure Michael J. 
Published: February 2025

ABSTRACTSynaptogenesis requires complex coordination between the terminating motor neuron and the developing myofiber endplate. Cross‐talk research has focused on in vivo models or singular treatments with known signaling molecules identified from these animal studies. However, in vivo models are inefficient at measuring dynamic signaling changes due to assay resolution and cost. Further, despite advances in culture methods relying on microfluidic platforms, much remains unknown about the dynamic cross‐talk between these two key cell types. As such, there is an unmet investigation into simple and reproducible coculture studies. In this study, we characterize both myoblast (C2C12) and motor neuron (NSC‐34) changes that occur in either a conditioned media model, a transwell coculture, and a 2D migration coculture. We successfully demonstrate repeatable changes in synaptogenesis with ~38% increase in Chrng protein levels (p < 0.05) in each model, increased myotube alignment in cocultured myoblasts measured with FFT analysis, and show motor neurons are preferentially chemo‐attracted to myotubes without the use of neurite‐path constraining microfluidics. Lastly, we identified a potential new signaling protein responsible for motor endplate development, apolipoprotein E (ApoE). This coculture approach reveals changes to myotube myogenesis and synaptogenesis providing a consistent platform for cross‐talk and pathway analysis for future studies.
Comparative Single‐Cell Analysis Reveals Tendon Progenitor Dysfunction by Age‐Associated Oxidative Stress and Its Restoration by Antioxidant Treatments
Authors: Jeong Youngjae , Yang Dongwook , Solidum Jea Giezl , Ortinau Laura , Park Dongsu 
Published: February 2025

ABSTRACTImpaired healing of adult tendons with fibrosis remains clinical challenges while neonatal tendons have full functional restoration. However, age‐associated cellular and molecular changes in tendon cells and tendon stem/progenitor cells (TSPCs) remain unknown. Here, comparative single cell transcriptomics of early postnatal (2 weeks old) and adult (20 weeks old) mouse tendons revealed that adult tendons have reduced number of TSPCs, decreased gene expression in tendon and cartilage development, and a greater population of fibro‐tenogenic cells. Notably, adult TSPCs and tenocytes exhibit increased expression of immune‐response and oxidative‐stress genes with higher EGFR but decreased IGF signaling. Adult tendon cells show increased levels of intracellular reactive oxygen species (ROS) in vivo. In contrast, antioxidant treatment of adult tendons significantly reduces intracellular ROS of TSPCs and improves tendon strength in vivo. Hence, these findings suggest that increased inflammation and ROS during tendon aging deteriorates tendon function and regeneration that can be mitigated by antioxidant treatment.
Association between early phosphate intake and refeeding syndrome in extremely low‐birth‐weight infants: A retrospective cohort study
Authors: Wright Thomas B. , Bloomfield Frank H. , Alexander Tanith , Cormack Barbara E. 
Published: February 2025

AbstractBackgroundPreterm infants are at risk of refeeding syndrome, a constellation of biochemical changes associated with nutrition. We aimed to determine whether increased early phosphate intake with routine biochemical monitoring is associated with a reduction in refeeding syndrome.MethodsRetrospective cohort study in two Auckland neonatal intensive care units comparing infants born <1000 g before (2014–2018, standard phosphate intake) and after (2020–2021, early phosphate intake) changes to intravenous nutrition protocols that increased phosphate intake and introduced biochemical monitoring. The standard phosphate intake cohort comprised the participants who received placebo in a randomized controlled trial of early increased amino acid intake. The early phosphate intake cohort was identified prospectively. Data were retrieved from the trial database or prospectively from electronic medical records. Groups were compared using either the chi‐square test or pooled t test and logistic or multiple logistic regression analysis. Refeeding syndrome was defined as concurrent hypophosphatemia (serum phosphate <1.4 mmol L−1) and hypercalcemia (serum calcium >2.8 mmol L−1).ResultsThe incidence of refeeding syndrome in the first 5 days after birth decreased from 11.9% to 2.9%, hypophosphatemia from 53.5% to 21.2%, and severe hypophosphatemia (<0.9 mmol L−1) from 11.3% to 1.2%. Probable early‐ and late‐onset sepsis reduced from 51.4% to 28.2% and from 62.5% to 28.0%, respectively.ConclusionsIncreased early phosphate intake with routine biochemical monitoring is associated with a lower incidence of refeeding syndrome, hypophosphatemia, and associated comorbidities. Whether these associations are causal requires further investigation.
Humidity‐Assisted High‐Resolution Laser Patterning of Perovskite Nanocrystals on Glass Beyond the Diffraction Limit
Authors: Wang Zhiqiang , Li Xinkuo , Chen Chenduan , Ruan Chao , Li Zengling , Zhai Renshuang , Sun Ke , Qiu Jianrong , Tan Dezhi 
Published: February 2025

AbstractLuminescent micro‐patterns of perovskite nanocrystals in glass written by femtosecond laser offer a novel alternative for integrated optoelectronic applications such as μ‐LED, image sensors, and optical communication. However, improving the resolution, specifically, reducing the sizes of pixel dots, of luminescent perovskite patterns directly processed by femtosecond laser in glass remains challenging due to the optical diffraction limit and the crystallographic nature of perovskites in glass. Herein, high‐resolution luminescent patterning is reported (with diameters of luminescent dots down to nanometer scale, beyond the diffraction limit) of perovskite nanocrystals on glass surfaces assisted by humidity treatment. The generation of photoluminescence (PL) emission from laser‐written non‐luminescent dot patterns is recognized as originating from the H2O‐promoted nanocrystallization of perovskites in the local region of pattern dots. By manipulating the humidity level or halide stoichiometry, arbitrary patterns with tunable emission colors can be fabricated. The current luminescent patterning strategy expanded practical applications in anti‐counterfeiting, information encryption/decryption, and optical storage. This work provides a high‐quality and versatile route toward high‐resolution perovskite patterns, which would unlock more new applications in optics and optoelectronics.
Recent Progress in CsPbX3 (X = Cl, Br, and I) Perovskite Quantum Dot Glasses: Synthesis, Matrix Optimization, and Photonic Application Potentials
Authors: Duan Yongmin , Gu Keyi , Li Shugang , Du Juan , Zhang Junjie 
Published: February 2025

AbstractPerovskite quantum dots (PQDs) have emerged as prominent candidates for a variety of optoelectronic applications, including solar cells, white light‐emitting diodes (WLED), and liquid crystal displays (LCD), owing to their remarkable optical and electronic properties. These properties encompass high absorption coefficients, elevated quantum yields, tunable bandgaps, and narrow emission peaks. Nonetheless, PQDs are inherently unstable due to their low formation energy and ionic crystal nature, making them susceptible to degradation upon exposure to light, heat, and moisture. To address these challenges, encapsulating PQDs within an inorganic glass matrix has been proven effective. The rigid structure of the glass matrix significantly enhances the stability of PQDs while preserving their exceptional optical characteristics. This review provides an overview of recent advancements in PQD glasses and aims to offer critical insights to propel future research in this domain. The commonly employed glass matrix and preparation techniques for PQD glasses are reviewed, highlighting their fundamental properties such as structural integrity, optical performance, andstability, and discussing the approaches to improve the optical properties of PQD glasses, which include glass component regulation and PQD structure regulation. Additionally, the potential applications of PQD glasses in diverse areas, including WLED, LCD, and temperature sensing are explored.
Performance Enhancement of Austenitic Stainless Steels Based on the Adjustment of Grain Size by Reversion, Recrystallization, and Grain Growth
Authors: Kalhor Alireza , Sohrabi Mohammad Javad , Mobasheri Mohammad , Rodak Kinga , Dehghanian Changiz , Mirzadeh Hamed 
Published: February 2025

ABSTRACTThe effects of grain size on the properties of AISI 304L and 316L stainless steels were critically discussed. Different grain sizes of 0.5, 2.7, 5.6, 15, 34, 90, and 192 μm were obtained via cold rolling followed by reversion, recrystallization, and grain growth annealing. Cyclic potentiodynamic polarization tests revealed that the uniform corrosion resistance deteriorated and the pitting resistance improved with grain refinement. Accordingly, a specific range at moderate grain sizes can be recommended to improve the overall corrosion behavior, which was in the range of 5.6 to 15 μm. Moreover, tensile tests revealed that the optimum strength‐ductility balance can be obtained at the grain size of 5.6 μm in both alloys. Accordingly, the grain sizes of ~5 μm can be considered as the optimum for both the strength‐ductility trade‐off and the balance between uniform and pitting corrosion resistance. While the uniform corrosion resistances of AISI 304L and 316L stainless steels were comparable, the latter alloy showed better pitting resistance due to the high Mo content, while the former alloy showed better strength‐ductility balance due to its high work‐hardening ability.
The Corrosion Resistance Study of Ni‐P‐CS Coating Improved by Carbon Nanoparticle
Authors: Liu Guiqun , Zhang Boyu , Wang Heming , Ma Qisheng , Lv Guanglei , Zhang Xiaoli , Wu Yishan , Li Ding 
Published: February 2025

ABSTRACTIn the present study, Ni‐P‐CS composite coating was prepared on Q235 carbon steel surface by electroless plating method. Candle soot (CS) is the soot produced by incomplete combustion of candles, and it is mainly composed of amorphous carbon and carbon nanoparticles. The results show that: the results indicated that the corrosion resistance of the Ni‐P deposit improved with the incorporation of candle soot (CS). The electrochemical test of Q235 carbon steel, Ni‐P and Ni‐P‐CS with 3.5% NaCl solution was performed at room temperature. The test results show that the protection efficiency of the new coating (Ni‐P‐CS) reaches 78.5%. Compared with the Ni‐P coating with the protection efficiency of 47.08%, its corrosion resistance increased by 31.42 percentage points. This study demonstrates for the first time that Ni‐P‐CS composite coating is a feasible method for metal corrosion prevention, with inexpensive raw materials and easy preparation. It provides a novel idea for petroleum, chemical and other metal anticorrosion fields.
Improved Galvanic Corrosion Model Considering the Corrosion Product Hindering Characteristic and Maximum Corrosion Depth
Authors: Han Shanling , Yin Jie , Li Xiaodong , Ping Kai , Li Yong 
Published: February 2025

ABSTRACTAccurate corrosion prediction model can help to warn corrosion problems in advance. Therefore, to predict the galvanic corrosion process, this paper introduces the maximum corrosion depth, proposes the concept of corrosion product hindering characteristic k, and establishes a novel semi‐empirical galvanic corrosion model. The model shows different corrosion states under different k values and is suitable for corrosion processes under different environments and materials. Through the multiple sets of corrosion data obtained from the self‐piercing riveted joints of carbon fiber reinforced composites and 5052 aluminum alloy under various corrosion cycles, the model under experimental conditions is solved, and the prediction effects of the other three models are compared. The new model has a coefficient of determination R2 = 0.9974, which is closest to 1, and MAE = 0.4973 is the smallest, so the model is considered effective.
Mechanism of Zinc Corrosion in Accelerated Corrosion Tests
Authors: Popova Kateryna , Prošek Tomáš 
Published: February 2025

ABSTRACTUnderstanding into the effect of parameters of cyclic accelerated corrosion tests on the corrosion response is essential for proper test design and interpretation of results. Real‐time corrosion rate of zinc was measured using electrical resistance sensors and statistically evaluated in seven tests. The chloride deposition phase was the most corrosive, influencing the corrosion behavior throughout the tests. Despite corrosion deceleration between chloride deposition events, zinc was repeatedly reactivated by the application of NaCl solution and zinc corrosion rate increased during the tests. It is in contrast to field observations. Prolonged exposure to high humidity and elevated temperature during the transition and wet phases accelerated corrosion, while prolonged drying at higher temperature inhibited corrosion. The results suggest that the corrosivity of future corrosion tests needs to be reduced by lowering the salt concentration, shortening the chloride deposition phase, and including sufficiently intense drying.
Knocking Down Ferredoxin 1 Inhibits the Progression of Colorectal Cancer and Regulates Cuproptosis via Mediating the Hippo Signaling Pathway
Authors: Hu Ying , Liu Haihua , Tan Xiaobin , Wu Xiongjian 
Published: February 2025

ABSTRACTCuproptosis is a form of programmed cell death dependent on mitochondrial respiration and is crucial in cancer treatment. The study attempted to screen cuproptosis‐associated genes in colorectal cancer (CRC) and reveal regulatory pathways. Weighted gene co‐expression network analysis (WGCNA) was applied to screen the co‐expression modules based on gene expression in CRC patients. The cuproptosis‐associated genes were screened at the intersection of co‐expression modules and cuproptosis gene data set. RNA sequencing was performed to assess the transcriptome changes, followed by functional enrichment analyses to reveal the potential pathways. Ferredoxin 1 (FDX1) was knocked down in in vivo and in vitro experiments to investigate the effects of FDX1 knockdown on CRC progression and cuproptosis. FDX1 was found as a cuproptosis‐associated gene and was highly expressed in CRC tumor and CRC cells. Knockdown of FDX1 regulated cuproptosis in CRC cells, and inhibited CRC cell growth, migration and invasion. We screened 1956 upregulated DEGs and 2201 downregulated DEGs in si‐FDX1 cells, which were mainly enriched in mitogen‐activated protein kinase (MAPK) signaling pathway, tumor necrosis factor (TNF) signaling pathway and Hippo signaling pathway. Knockdown of FDX1 inhibited CRC progression by increasing the levels of dihydrolipoamide S‐succinyltransferase (DLST), lipoic acid synthetase (LIAS) and phosphorylation Yes‐associated protein (pYAP)/YAP, and downregulated transcriptional coactivator with a PDZ‐binding domain (TAZ). The inhibitor of Hippo pathway GA‐017 blocked this process. Knocking down FDX1 regulated cuproptosis and inhibited CRC progression by mediating the Hippo signaling pathway, which shed new insights into the development of biomarkers for CRC treatment.