bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of an epicuticular wax crystal deficiency gene Brwdm1 in Chinese cabbage (Brassica campestris L. ssp. pekinensis)
Authors: Song Gengxing , Liu Chuanhong , Fang Bing , Ren Jie , Feng Hui 
Published: June 2023

IntroductionThe cuticle wax covering the plant surface is a whitish hydrophobic protective barrier in Chinese cabbage, and the epicuticular wax crystal deficiency normally has higher commodity value for a tender texture and glossy appearance. Herein, two allelic epicuticular wax crystal deficiency mutants, wdm1 and wdm7, were obtained from the EMS mutagenesis population of a Chinese cabbage DH line ‘FT’.MethodsThe cuticle wax morphology was observed by Cryo-scanning electron microscopy (Cryo-SEM) and the composition of wax was determined by GC-MS. The candidate mutant gene was found by MutMap and validated by KASP. The function of candidate gene was verified by allelic variation.ResultsThe mutants had fewer wax crystals and lower leaf primary alcohol and ester content. Genetic analysis revealed that the epicuticular wax crystal deficiency phenotype was controlled by a recessive nuclear gene, named Brwdm1. MutMap and KASP analyses indicated that BraA01g004350.3C, encoding an alcohol-forming fatty acyl-CoA reductase, was the candidate gene for Brwdm1. A SNP 2,113,772 (C to T) variation in the 6th exon of Brwdm1 in wdm1 led to the 262nd amino acid substitution from threonine (T) to isoleucine (I), which existed in a rather conserved site among the amino acid sequences from Brwdm1 and its homologs. Meanwhile, the substitution changed the three-dimensional structure of Brwdm1. The SNP 2,114,994 (G to A) in the 10th exon of Brwdm1 in wdm7 resulted in the change of the 434th amino acid from valine (V) to isoleucine (I), which occurred in the STERILE domain. KASP genotyping showed that SNP 2,114,994 was co-segregated with glossy phenotype. Compared with the wild type, the relative expression of Brwdm1 was significantly decreased in the leaves, flowers, buds and siliques of wdm1.DiscussionThese results indicated that Brwdm1 was indispensable for the wax crystals formation and its mutation resulted in glossy appearance in Chinese cabbage.
The patients‘ perspective - a qualitative analysis of experiencing a fracture-related infection
Authors: Wimalan Bravena , Rupp Markus , Alt Volker , Walter Nike 
Published: June 2023

IntroductionFracture-related infection is a devastating complication confronting the patient with several challenges. To improve the management and to enhance the patients’ wellbeing the focus of this study was to understand the emotional impact and patients’ experiences during the process to identify challenges, difficulties, and resources. For this, a qualitative content analysis of semi-structured interviews according to Graneheim and Lundman was performed.MethodsIn total n = 20 patients of a German university orthopedic trauma centre specialized in bone and joint infections were recruited using a purposive sampling strategy. The patients were treated at the hospital between 2019 and 2021 and underwent at least one surgery. Individual in-person interviews were performed by one researcher based on a semi-structured guide, which was previously conceptualized. Content analysis according to Graneheim and Lundman was performed on the transcripts by two of the researchers independently.ResultsThe following major themes emerged: (i) the emotional and mental aspects highlighting the fact that FRI patients faced severe restrictions in their day-to-day life, which resulted in dependency on others and frustration, as well as future concerns showing that patients could not overcome a state of anxiety and fear even after successful treatment, (ii) socioeconomic consequences confronting patients with consequences on the job and in finances where they often feel helpless, and (iii) resources emphasizing the role of spirituality as a coping strategy and yoga exercises for keeping the positivity.ConclusionThis study emphasized the challenge of fracture-related infection management and associated consequences from the patients’ perspective. Not being well informed about possible negative outcomes or restrictions makes it harder for patients to accept the situation and patients expressed a need for better information and certainty. Also, patients developed constant anxiety and other psychological disturbances, highlighting the potential benefit of psychological support and patient-peer support to exchange experiences.
Developing a mechanism-based therapy for acute psychiatric inpatients with psychotic symptoms: an Intervention Mapping approach
Authors: Gussmann Eva , Lucae Susanne , Falkai Peter , Padberg Frank , Egli Samy , Kopf-Beck Johannes 
Published: June 2023

BackgroundTreatment guidelines for psychosis recommend offering psychotherapy already in the acute illness phase. However, there is a lack of available interventions adapted to the specific needs and key change mechanisms of inpatients experiencing severe symptoms and crisis. In this article we outline the scientific development process of a needs-oriented and mechanism-based group intervention for acute psychiatric inpatients with psychosis (MEBASp).MethodsTo guide our intervention design, we used Intervention Mapping (IM), a six-step framework for developing evidence-based health interventions that consisted of an extensive literature review, an in-depth problem definition and needs analysis, the modeling of change mechanisms and outcomes and the production of an intervention prototype.ResultsOur low-threshold modularized group intervention consists of nine stand-alone sessions (two per week) within three modules and targets different aspects of metacognitive and social change mechanisms. Module I and II aim to reduce acute symptoms by fostering cognitive insight, Module III focuses on reducing distress via cognitive defusion. Therapy contents are adapted from existing metacognitive treatments such as the Metacognitive Training and presented in a destigmatizing, simply understandable and experience-oriented way.ConclusionMEBASp is currently evaluated in a single-arm feasibility trial. Using a systematic and rigorous development methodology and providing a detailed description of the development steps demonstrated to be invaluable in improving the intervention’s scientific foundation, validity, and replicability for similar research.
The association between childhood trauma and adolescent cyberbullying: chain mediating roles of emotional intelligence and online social anxiety
Authors: Cao Guanghai , Wei Xinyu , Liu Juan , Li Xianyin 
Published: June 2023

ObjectiveThis study explored the impact of childhood trauma on adolescent cyberbullying and the mediating roles of emotional intelligence and online social anxiety between them.MethodsThe Childhood Trauma Scale, Emotional Intelligence Scale, and Chinese Brief Version of the Social Media User Social Anxiety Scale and Cyber Bullying Scale were used to assess 1,046 adolescents [boys: 297, girls: 749, average age = 15.79 years] from four schools in Shandong Province, China. SPSS 25.0 and AMOS 24.0 were used for statistical analysis.Results(1) Childhood trauma was positively associated with adolescents’ cyberbullying; (2) Emotional intelligence and online social anxiety played partial mediating roles in the relationship between childhood trauma and cyberbullying; (3) Emotional intelligence and online social anxiety played a chain mediating role in the relationship between childhood trauma and cyberbullying.ConclusionThis study reveals the relationship and mediating mechanisms between childhood trauma and cyberbullying. It provides implications for the theory and prevention of cyberbullying.
Understanding the roles of the healthcare and child welfare systems in promoting the safety and well-being of children
Authors: Joh-Carnella Nicolette , Livingston Eliza , Kagan-Cassidy Miya , Vandermorris Ashley , Smith Jennifer N. , Lindberg Daniel M. , Fallon Barbara 
Published: June 2023

IntroductionThe accurate identification and appropriate investigation of child maltreatment is a key priority for promoting the optimal health and development of children. Healthcare providers are often well-positioned professionals to report suspected child abuse and neglect, and, therefore, interact regularly with child welfare workers. Little research has examined the relationship between these two groups of professionals.MethodsWe interviewed healthcare providers and child welfare workers in order to examine the referral and child welfare investigation processes to understand strengths and identify areas of improvement for future collaboration. Thirteen child welfare workers from child welfare agencies and eight healthcare providers from a pediatric tertiary care hospital in Ontario, Canada were interviewed to meet the study’s objectives.ResultsHealthcare providers spoke about positive experiences making reports, factors impacting reporting decisions, areas for improvement (e.g., difficulties communicating, lack of collaboration, and disruption of therapeutic alliance), training, and professional roles. For interviews with child welfare workers, identified themes included healthcare professionals’ perceived expertise and understanding the role of child welfare. Both groups brought up the need for increased collaboration as well as systemic barriers and legacies of harm.DiscussionOur core finding was a reported lack of communication between the groups of professionals. Other identified barriers in collaboration included a lack of understanding of each other’s roles, hesitation for healthcare providers making reports, as well as legacies of harm and systemic inequities in both institutions. Future research should build on this examination by including the voices of healthcare providers and child welfare workers to identify sustainable solutions for increased collaboration.
Medical malpractice in hospitals—how healthcare staff feel
Authors: Tsai Shang-Feng , Wu Chieh-Liang , Ho Yu-Ying , Lin Pei-Yi , Yao Ai-Chu , Yah Ya-Hui , Hsiao Chia-Min , You Yu Huei , Yeh Te-Feng , Chen Cheng-Hsu 
Published: June 2023

IntroductionLiterature is limited on quantified acute stress reaction, the impact of event scale on medical staff when facing medical malpractice (MMP), and how to individually care for staff.MethodsWe analyzed data in the Taichung Veterans General Hospital from October 2015 to December 2017, using the Stanford Acute Stress Reaction Questionnaire (SASRQ), the Impact of Event Scale-Revised (IES-R), and the medical malpractice stress syndrome (MMSS).Results and DiscussionOf all 98 participants, most (78.8%) were women. Most MMPs (74.5%) did not involve injury to patients, and most staff (85.7%) indicated receiving help from the hospital. The internal-consistency evaluations of the three questionnaires showed good validity and reliability. The highest score of IES-R was the construct of intrusion (30.1); the most severe construct of SASRQ was “Marked symptoms of anxiety or increased arousal,” and the most were having mental and mild physical symptoms for MMES. A higher total IES-R was associated with younger age (&lt;40 y/o), and more severe injury on patients (mortality). Those who indicated receiving very much help from the hospital were those having significantly lower SASRQ sores. Our study highlighted that hospital authorities should regularly follow up on staff’s response to MMP. With timely interventions, vicious cycles of bad feelings can be avoided, especially in young, non-doctor, and non-administrative staff.
Effect of scattering angle on DSCOVR/EPIC observations
Authors: Wen Guoyong , Marshak Alexander 
Published: June 2023

The Earth Polychromatic Imaging Camera (EPIC) on the Deep Space Climate Observatory (DSCOVR) routinely captures reflected radiation from the whole sunlit side of the Earth in the near backward direction to monitor the changing planet. The instrument had routinely operated until 27 June 2019, when the spacecraft was placed in an extended safe hold due to degradation of an inertial navigation unit. DSCOVR returned to full operations on 2 March 2020. Since then, the range of scattering angles between the incident sunlight and sensor direction has been larger than before and the largest scattering angle reaches ∼178°, only 2° from perfect backscattering, proving a unique opportunity to study the top-of-atmosphere (TOA) reflectance under such extreme conditions. In the paper, we compare EPIC global spectral reflectances in 2021–2016. We found that there are four occasions when the scattering angle reaches about 178° and associated with them enhanced global daily average spectral reflectances in 2021. The scattering angle related reflectance enhancements are not found in 2016 data when the maximum scattering angle is about 174.5°. CERES data do not show such occasions in global daily reflected shortwave flux. As a result, those enhanced reflectance occasions are primarily due to the change in scattering angle. The enhancement due to changes in scattering angle depends strongly on wavelength, primarily because of wavelength dependence of cloud scattering phase function. Radiative transfer calculations show that the change in scattering angles has the largest impact on reflectance in the red and NIR channels at 680 nm and 780 nm and the smallest influence on reflectance in the UV channel at 388 nm, consistent with EPIC observations. The change of global average cloud amount also plays an important role in the reflectance enhancement. The influence of the cloud effect depends on whether the change is in phase or not with the change of scattering angle.
Case report: Shock after percutaneous vertebroplasty of the 5th thoracic vertebra
Authors: Hao Shenshen , Yu Xin , Li Laihao , Liu Shuai , Li Hongke , Dong Shengli , Cao Xinhao 
Published: June 2023

BackgroundPercutaneous vertebroplasty (PVP) is a common treatment for osteoporotic vertebral compression fracture (OVCF). Perioperative bleeding is usually rare, so there are few reports of shock. However, we developed shock after treating a case of OVCF of the 5th thoracic vertebra with PVP.Case presentationAn 80 years old female patient received PVP due to OVCF of the 5th thoracic vertebra. The operation was successfully completed and the patient returned to the ward safely after the operation. At 90 min after operation, she developed shock, which was induced by subcutaneous hemorrhage up to 1500 ml at the puncture site. Before using vascular embolization, transfusion and blood transfusion were used to maintain blood pressure, and local ice bag compression was used to reduce swelling and stop bleeding, which achieved successful hemostasis. She recovered and discharged after 15 days, with the hematoma having absorbed. There was no recurrence during the 17-month follow-up.ConclusionAlthough PVP is considered to be a safe and effective method to treat OVCF, the possible hemorrhagic shock still needs to arouse the vigilance of surgeons.
Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults
Authors: Trujillo-Peralta Carolina , Latorre Juan David , Chai Jianmin , Senas-Cuesta Roberto , Forga Aaron , Coles Makenly , Zhao Jiangchao , Hernandez-Velasco Xochitl , Tellez-Isaias Guillermo , Barta John , Bielke Lisa , Hargis Billy , Graham Danielle 
Published: June 2023

IntroductionDrug-sensitive live coccidiosis vaccines have been used to control coccidiosis and renew drug sensitivity in commercial chicken operations. However, only limited species coverage vaccines have been available for commercial turkey producers. This study aimed to assess the effect of an E. meleagrimitis vaccine candidate, with and without amprolium intervention, on performance and oocyst shedding. Additionally, the effect of vaccination, amprolium treatment, and E. meleagrimitis challenge on intestinal integrity and microbiome composition was evaluated.MethodsExperimental groups included: (1) NC (non-vaccinated, non-challenged control); (2) PC (non-vaccinated, challenged control); (3) VX + Amprol (E. meleagrimitis candidate vaccine + amprolium); and 4) VX (E. meleagrimitis candidate vaccine). For VX groups, 50% of the direct poults were orally vaccinated at DOH with 50 sporulated E. meleagrimitis oocysts and were comingled with contact or non-vaccinated poults for the duration of the study. From d10-14, VX + Amprol group received amprolium (0.024%) in the drinking water. All groups except NC were orally challenged with 95K E. meleagrimitis sporulated oocysts/mL/poult at d23. At d29, ileal and cecal contents were collected for 16S rRNA gene-based microbiome analysis.Results and DiscussionVX did not affect performance during the pre-challenge period. At d23-29 (post-challenge), VX groups had significantly (P &lt; 0.05) higher BWG than the PC group. Contacts and directs of VX groups in LS had significantly reduced compared to PC. As anticipated, amprolium treatment markedly reduced fecal and litter OPG for the VX + Amprol group compared to the VX group which did not receive amprolium. The ileal and cecal content results showed that the PC group had different bacterial diversity and structure, including alpha and beta diversity, compared to NC. Linear discriminant analysis Effect Size (LEfSe) identified that Lactobacillus salivarius (ASV2) was enriched in PC’s ileal and cecal content. Compared to NC and PC, the vaccinated groups showed no distinct clusters, but there were similarities in the ileal and cecal communities based on Bray-Curtis and Jaccard distances. In conclusion, these results indicate that vaccination with this strain of E. meleagrimitis, with or without amprolium intervention, caused a very mild infection that induced protective immunity and challenge markedly affected both the ileal and cecal microbiome.
Evaluation of the effect of the indoor environment on the physiological responses of early-gestation sows in a commercial house in China
Authors: Li Yangyang , Li Tong , Shang Bin , Zhao Yang , Tao Xiuping , Peng Feng , Zou Xiaojun , Zhang Sixin 
Published: June 2023

ObjectiveThe environment influences the sow's health and physiology during gestation. This study was conducted to evaluate indoor environmental parameters and physiological responses of early-gestation sows and investigate the possible methods for assessing the thermal environment in commercial houses.MethodsA total of 20 early-gestation sows (commercial purebred Yorkshire) with an average body weight of 193.20 ± 3.62 kg were used for this study in winter, spring, summer, and autumn. The indoor environment parameters comprising dry-bulb temperature (Tdb), relative humidity (RH), and carbon dioxide (CO2) were recorded in 30-min intervals. Physiological parameters including heart rate (HR) and respiration rate (RR) of sows were also measured every 30 min. Wet-bulb temperature (Twb) was calculated using Tdb, RH and atmospheric pressure was recorded at a nearby weather station.ResultsThe average indoor Tdb and RH were 12.98 ± 2.03°C and 80.4 ± 6.4% in winter, 18.98 ± 2.68°C and 74.4 ± 9.0% in spring, 27.49 ± 2.05°C and 90.6 ± 6.4% in summer, and 17.10 ± 2.72°C and 64.5 ± 10.9% in autumn. A higher average concentration of CO2 was observed in winter (1,493 ± 578 mg/m3) than in spring (1,299 ± 489 mg/m3), autumn (1,269 ± 229 mg/m3), and summer (702 ± 128 mg/m3). Compared with the HR and RR in the optimum environment, high RH in the house led to a significant decrease in both HR and RR (P &lt; 0.05). In addition, a significant decline in HR was also obtained at high temperatures (P &lt; 0.05). A temperature humidity index (THI), THI = 0.82 × Tdb + 0.18 × Twb, was determined for early-gestation sows, and the THI thresholds were 25.6 for HR. The variation in THI in summer showed that heat stress still occurred under the pad-fan cooling system.ConclusionThis study demonstrated the critical significance of considering physiological responses of early-gestation sows in commercial houses and THI thresholds. We recommend that much more cooling measures should be taken for early-gestation sows in summer.
Ensino de bioquímica: uma abordagem fundamentada nas premissas do movimento CTS
Authors: Ferreira Luan Daniel Silva , Soares Raimundo Rafael de Sousa , Da Silva Luan Cleyton Ramos , Dos Santos Geovana Silva , Sobrinho Dábila Carla de Almeida , Filho Antonio Carlos Monteiro , Tavares Romário da Silva 
Published: June 2023

Na atualidade, as sociedades passam por várias transformações de caráter: econômico, político, social e cultural. Essas transformações afetam a educação levando a uma reflexão sobre o papel das escolas e dos professores no processo educacional. Diante disso, o objetivo do estudo foi analisar a aplicabilidade de uma sequência didática (SD) para trabalhar conteúdos de Bioquímica, na educação básica, articulando Ciência-Tecnologia-Sociedade (CTS). A SD foi planejada e executada no formato de um minicurso em uma turma de ensino médio da rede pública de educação. Para tanto, foi utilizada uma abordagem de pesquisa qualitativa. Para a coleta dos dados, foram utilizados questionários e a observação. Os dados foram analisados à luz de três categorias: i) Articulação da tríade CTS; ii) Metodologia de ensino e recursos didáticos utilizados; iii) Percepções dos 3alunos sobre o processo de ensino-aprendizagem desenvolvido. Dessa forma, se fez necessário promover uma atmosfera de interação, discussão, reflexão e dialogicidade durante as aulas, possibilitando um processo educativo visando à cidadania. Diante dos resultados, observou-se que os estudantes avaliaram de maneira positiva a proposta de ensino de Bioquímica com enfoque CTS e se mostraram satisfeitos com as metodologias de ensino e com os recursos didáticos utilizados.
Humanização do cuidado sob a ótica de enfermeiros de uma unidade neonatal
Authors: Tavares Rosangela Cunha Machado , Reis Danielle Bonotto Cabral , De Camargo Fátima Cristina Mattara , Tavares Tainan Maria Cruz Lopes , Vieira Claudia Cristina Augusto Rodrigues , Menaguali Richely Ritta , De Oliveira Rosane , Olivo Katlen Susane da Silva 
Published: June 2023

a humanização pode ser compreendida como uma iniciativa que valoriza a excelência do cuidado. No que tende ao cuidado de recém-nascidos, as unidades neonatais têm como principal objetivo prover recursos contínuos, priorizando as melhores práticas, propondo estratégias individualizadas, respeitando as particularidades e necessidades de cada um. Aliando, assim, a personalidade humanizada às ações dos profissionais de saúde. Objetivos: descrever o perfil de enfermeiros que atuam em uma unidade neonatal; analisar as práticas humanizadas realizadas em uma unidade neonatal; e discutir a visão dos enfermeiros acerca da humanização neonatal. Metodologia: pesquisa descritiva, exploratória, de abordagem qualitativa, realizada em um instituto nacional de saúde. Operacionalizada por meio de formulário e entrevista semiestruturada. Resultados: as estratégias de humanização mais citadas, pelas entrevistadas foram a questão da inclusão familiar no cuidado, o aconchego do bebê e a sucção não nutritiva. Conclusão: percebeu-se uma equipe com graus de entendimento variados sobre a temática. Além de uma dificuldade, de alguns profissionais, em estabelecer interface da teoria com a prática, limitando a utilização das ferramentas de humanização. Notou-se, também, uma grande referência à inserção da família no cuidado como estratégia, além da hipervalorização da relação mãe-bebê em detrimento da paterna, evidenciando a necessidade de apropriação da temática por partes dos enfermeiros, para uma melhor aplicação.