bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cobalt–Samarium Oxide Composite as a Novel High-Performance Catalyst for Partial Oxidation and Dry Reforming of Methane into Synthesis Gas
Authors: Loktev A. S. , Arkhipova V. A. , Bykov M. A. , Sadovnikov A. A. , Dedov A. G. 
Published: February 2023

Abstract
The paper describes a novel high-performance catalyst that was developed for partial oxidation of methane (POM) and dry reforming of methane (DRM) into synthesis gas. The catalyst is based on samarium cobaltite dispersed in a samarium oxide matrix. Unlike its known counterparts based on samarium cobaltate, the novel catalyst is resistant to carbonization and contains active sites that exhibit higher syngas productivity.
Pediatric Extracorporeal Cardiopulmonary Resuscitation: Development of a Porcine Model and the Influence of Cardiopulmonary Resuscitation Duration on Brain Injury
Authors: Slovis Julia C. , Volk Lindsay , Mavroudis Constantine , Hefti Marco , Landis William P. , Roberts Anna L. , Delso Nile , Hallowell Thomas , Graham Kathryn , Starr Jonathan , Lin Yuxi , Melchior Richard , Nadkarni Vinay , Sutton Robert M. , Berg Robert A. , Piel Sarah , Morgan Ryan W. , Kilbaugh Todd J. 
Published: February 2023

Background
The primary objective was to develop a porcine model of prolonged (30 or 60 minutes) pediatric cardiopulmonary resuscitation (CPR) followed by 22‐ to 24‐hour survival with extracorporeal life support, and secondarily to evaluate differences in neurologic injury.

Methods and Results

Ten‐kilogram, 4‐week‐old female piglets were used. First, model development established the technique (n=8). Then, a pilot study was conducted (n=15). After 80% survival was achieved in the final 5 pilot animals, a proof‐of‐concept randomized study was completed (n=11). Shams (n=6) underwent anesthesia only. Severe neurological injury was determined by a composite score of mitochondrial function, neuropathology, and cerebral metabolism: scale of 0–6 (severe: >3). Among 15 piglets in the pilot study, overall survival was 10 (67%); of the final 5, overall survival was 4 (80%). Eleven piglets were then randomized to 60 (CPR60, n=5) or 30 minutes of CPR (CPR30, n=5); 1 animal was excluded from prerandomization for intra‐abdominal hemorrhage (10/11, 91% survival). Three of 5 animals in the CPR60 group had severe neurological injury scores versus 1 of 5 in the CPR30 group (
P
=0.52). During ECMO, CPR60 animals had lower pH (CPR60: 7.4 [IQR 7.4–7.4] versus CPR30: 7.5 [IQR 7.4–7.5],
P
=0.022), higher lactate (CPR60: 6.8 [IQR 6.8–11] versus CPR30: 4.2 [IQR 4.1–4.3] mmol/L;
P
=0.012), and higher ICP (CPR60: 19.3 [IQR 11.7–29.3] versus CPR30: 7.9 [IQR 6.7–9.3] mm Hg;
P
=0.037). Both groups had greater mitochondrial injury than shams (CPR60:
P
<0.001; CPR30:
P
<0.001). CPR60 did not differ from CPR30 in mitochondrial respiration, neuropathology, or cerebral metabolism.

Conclusions
A pediatric porcine model of extracorporeal cardiopulmonary resuscitation after 60 and 30 minutes of CPR consistently resulted in 24‐hour survival with more severe lactic acidosis in the 60‐minute cohort.
Association Between Triglycerides and Incident Cognitive Impairment in Black and White Adults in the Reasons for Geographic and Racial Differences in Stroke Study
Authors: Rosenson Robert S. , Cushman Mary , McKinley Emily C. , Muntner Paul , Wang Zhixin , Vaisar Tomas , Heinecke Jay , Tangney Christy , Judd Suzanne , Colantonio Lisandro D. 
Published: February 2023

Background
Elevated nonfasting triglycerides were associated with non‐Alzheimer dementia in a recent study. However, this study neither evaluated the association of fasting triglycerides with incident cognitive impairment (ICI) nor adjusted for high‐density lipoprotein cholesterol or hs‐CRP (high‐sensitivity C‐reactive protein), known risk markers for ICI and dementia.

Methods and Results
We examined the association between fasting triglycerides and ICI among 16 170 participants in the REGARDS (Reasons for Geographic and Racial Differences in Stroke) study without cognitive impairment or a history of stroke at baseline in 2003 to 2007 and who had no stroke events during follow‐up through September 2018. Overall, 1151 participants developed ICI during the median follow‐up of 9.6 years. The relative risk for ICI associated with fasting triglycerides of ≥150 mg/dL versus <100 mg/dL including adjustment for age and geographic region of residence was 1.59 (95% CI, 1.20–2.11) among White women and 1.27 (95% CI, 1.00–1.62) among Black women. After multivariable adjustment, including adjustment for high‐density lipoprotein cholesterol and hs‐CRP, the relative risk for ICI associated with fasting triglycerides ≥150 mg/dL versus <100 mg/dL was 1.50 (95% CI, 1.09–2.06) among White women and 1.21 (95% CI, 0.93–1.57) among Black women. There was no evidence of an association between triglycerides and ICI among White or Black men.

Conclusions
Elevated fasting triglycerides were associated with ICI in White women after full adjustment including high‐density lipoprotein cholesterol and hs‐CRP. The current results suggest that the association between triglycerides and ICI is stronger in women than men.
Trends and Costs of US Telehealth Use Among Patients With Cardiovascular Disease Before and During the COVID‐19 Pandemic
Authors: Lee Jun Soo , Lowe Beasley Kincaid , Schooley Michael W. , Luo Feijun 
Published: February 2023

Background
The COVID‐19 pandemic affected outpatient care delivery and patients' access to health care. However, no prior studies have documented telehealth use among patients with cardiovascular disease.

Methods and Results
We documented the number of telehealth and in‐person outpatient encounters per 100 patients with cardiovascular disease and the percentage of telehealth encounters from January 2019 to June 2021, and the average payments per telehealth and in‐person encounters across a 12‐month period (July 2020–June 2021) using the MarketScan commercial database. From February 2020 to April 2020, the number of in‐person encounters per 100 patients with cardiovascular disease decreased from 304.2 to 147.7, whereas that of telehealth encounters increased from 0.29 to 25.3. The number of in‐person outpatient encounters then increased to 280.7 in June 2020, fluctuated between 268.1 and 346.4 afterward, and ended at 268.1 in June 2021, lower than the prepandemic levels. The number of telehealth encounters dropped to 16.8 in June 2020, fluctuated between 8.8 and 16.6 afterward, and ended at 8.8 in June 2021, higher than the prepandemic levels. Patients who were aged 18 to 35 years, women, and living in urban areas had higher percentages of telehealth encounters than those who were aged 35 to 64 years, men, and living in rural areas, respectively. The mean (95% CI) telehealth and in‐person outpatient encounter costs per visit were $112.8 (95% CI, $112.4–$113.2) and $161.4 (95% CI, $160.4– $162.4), respectively.

Conclusions
There were large fluctuations in telehealth and in‐person outpatient encounters during the pandemic. Our results provide insight into increased telehealth use among patients with cardiovascular disease after telehealth policy changes were implemented during the pandemic.
A multicenter analysis of the outcomes with venetoclax in patients with relapsed mantle cell lymphoma
Authors: Sawalha Yazeed , Goyal Subir , Switchenko Jeffrey M. , Romancik Jason T. , Kamdar Manali , Greenwell I. Brian , Hess Brian T. , Isaac Krista M. , Portell Craig A. , Mejia Garcia Alex , Goldsmith Scott , Grover Natalie S. , Riedell Peter A. , Karmali Reem , Burkart Madelyn , Buege Michael , Akhtar Othman , Torka Pallawi , Kumar Anita , Hill Brian T. , Kahl Brad S. , Cohen Jonathon B. 
Published: February 2023

Abstract
To report the activity of venetoclax in patients with relapsed mantle cell lymphoma (MCL), we identified 81 patients treated with venetoclax monotherapy (n = 50, 62%) or in combination with a Bruton tyrosine kinase inhibitor (BTKi) (n = 16, 20%), an anti-CD20 monoclonal antibody (n = 11, 14%), or other active agents at 12 US academic medical centers. Patients had high-risk disease features including Ki67 &gt;30% (61%), blastoid/pleomorphic histology (29%), complex karyotype (34%), and TP53 alterations (49%), and received a median of 3 prior treatments including BTKis in 91%. Venetoclax alone or in combination resulted in an overall response rate (ORR) of 40% and median progression-free (PFS) and overall survival (OS) of 3.7 and 12.5 months, respectively. The receipt of ≤3 prior treatments was associated with higher odds of response to venetoclax in a univariable analysis. In a multivariable analysis, having a high-risk Mantle Cell Lymphoma International Prognostic Index score before receiving venetoclax and disease relapse or progression within 24 months of diagnosis were associated with inferior OS whereas the use of venetoclax in combination was associated with superior OS. Although most patients (61%) had low risk for tumor lysis syndrome (TLS), 12.3% of patients developed TLS despite the implementation of several mitigation strategies. In conclusion, venetoclax resulted in good ORR but short PFS in patients with MCL who are at high risk, and may have a better role in earlier lines of treatment and/or in conation with other active agents. TLS remains an important risk in patients with MCL who initiate treatment with venetoclax.
Physioxia improves the selectivity of hematopoietic stem cell expansion cultures
Authors: Igarashi Kyomi J. , Kucinski Iwo , Chan Yan Yi , Tan Tze-Kai , Khoo Hwei Minn , Kealy David , Bhadury Joydeep , Hsu Ian , Ho Pui Yan , Niizuma Kouta , Hickey John W. , Nolan Garry P. , Bridge Katherine S. , Czechowicz Agnieszka , Gottgens Berthold , Nakauchi Hiromitsu , Wilkinson Adam C. 
Published: February 2023

Abstract
Hematopoietic stem cells (HSCs) are a rare type of hematopoietic cell that can entirely reconstitute the blood and immune system after transplantation. Allogeneic HSC transplantation (HSCT) is used clinically as a curative therapy for a range of hematolymphoid diseases; however, it remains a high-risk therapy because of its potential side effects, including poor graft function and graft-versus-host disease (GVHD). Ex vivo HSC expansion has been suggested as an approach to improve hematopoietic reconstitution in low-cell dose grafts. Here, we demonstrate that the selectivity of polyvinyl alcohol (PVA)-based mouse HSC cultures can be improved using physioxic culture conditions. Single-cell transcriptomic analysis helped confirm the inhibition of lineage-committed progenitor cells in physioxic cultures. Long-term physioxic expansion also afforded culture-based ex vivo HSC selection from whole bone marrow, spleen, and embryonic tissues. Furthermore, we provide evidence that HSC-selective ex vivo cultures deplete GVHD-causing T cells and that this approach can be combined with genotoxic-free antibody-based conditioning HSCT approaches. Our results offer a simple approach to improve PVA-based HSC cultures and the underlying molecular phenotype, and highlight the potential translational implications of selective HSC expansion systems for allogeneic HSCT.
Performance improvement of a tunnel junction memristor with amorphous insulator film
Authors: Liu Fenning , Peng Yue , Liu Yan , Xiao Wenwu , Hao Yue , Han Genquan 
Published: February 2023

AbstractThis study theoretically demonstrated the oxygen vacancy (VO2+)-based modulation of a tunneling junction memristor (TJM) with a high and tunable tunneling electroresistance (TER) ratio. The tunneling barrier height and width are modulated by the VO2+-related dipoles, and the ON and OFF-state of the device are achieved by the accumulation of VO2+ and negative charges near the semiconductor electrode, respectively. Furthemore, the TER ratio of TJMs can be tuned by varying the density of the ion dipoles (Ndipole), thicknesses of ferroelectric-like film (TFE) and SiO2 (Tox), doping concentration (Nd) of the semiconductor electrode, and the workfunction of the top electrode (TE). An optimized TER ratio can be achieved with high oxygen vacancy density, relatively thick TFE, thin Tox, small Nd, and moderate TE workfunction.
Identification and analysis of miRNAs differentially expressed in male and female Trichosanthes kirilowii maxim
Authors: Hu Xiu-qin , Song Han , Li Na , Hao Chun-xiang , Zhang Bo , Li Xin-peng , Xin Jie , Zhang Yong-qing 
Published: February 2023

AbstractTrichosanthes kirilowii Maxim. (TK) is a dioecious plant in the Cucurbitaceae family of which different sexes have separate medicinal uses. We used Illumina high-throughput sequencing technology to sequence miRNAs from male and female flower buds of TK. We performed bioinformatics analysis, miRNA identification, and target gene prediction on the data obtained from sequencing, and association analysis was performed in combination with the results of a previous transcriptome sequencing study. As a result, there were 80 differentially expressed miRNAs (DESs) between the female and male plants (48 upregulated and 32 downregulated in female plants). Moreover, 27 novel miRNAs in DESs were predicted to have 282 target genes, and 51 known miRNAs were predicted to have 3418 target genes. By establishing a regulatory network between miRNAs and target genes, 12 core genes were screened, including 7 miRNAs and 5 target genes. Among them, tkmiR157a-5p, tkmiR156c, tkmiR156_2, and tkmiR156k_2 jointly target the regulation of tkSPL18 and tkSPL13B. These two target genes are specifically expressed in male and female plants, respectively, and are involved in the biosynthesis process of BR, which is closely related to the sex differentiation process of TK. The identification of these miRNAs will provide a reference for the analysis of the sex differentiation mechanism of TK.
TRAP-induced PAR1 expression with its mechanism during AMI in a rat model
Authors: Wang Ani , Gu Xinyuan , Wang Chunyang , Li Yanhui , Deng Fuhong , Fang Jie , Chen Naxia , Li Qifu , Tang Lilong 
Published: February 2023

Abstract
Background and objective
Protease-activated receptor 1 (PAR1) is crucial in individuals with acute myocardial infarction (AMI). The continuous and prompt PAR1 activation mainly dependent on PAR1 trafficking is essential for the role of PAR1 during AMI in which cardiomyocytes are in hypoxia. However, the PAR1 trafficking in cardiomyocytes specially during the hypoxia is still unclear.

Methods and result
A rat AMI model was created. PAR1 activation with thrombin-receptor activated peptide (TRAP) had a transient effect on cardiac function in normal rats but persistent improvement in rats with AMI. Cardiomyocytes from neonatal rats were cultured in a normal CO2 incubator and a hypoxic modular incubator chamber. The cells were then subjected to western blot for the total protein expression and staining with fluorescent reagent and antibody for PAR1 localization. No change in total PAR1 expression following TRAP stimulation was observed; however, it led to increased PAR1 expression in the early endosomes in normoxic cells and decreased expression in the early endosomes in hypoxic cells. Under hypoxic conditions, TRAP restored the PAR1 expression on both cell and endosomal surfaces within an hour by decreasing Rab11A (8.5-fold; 179.93 ± 9.82% of the normoxic control group, n = 5) and increasing Rab11B (15.5-fold) expression after 4 h of hypoxia. Similarly, Rab11A knockdown upregulated PAR1 expression under normoxia, and Rab11B knockdown downregulated PAR1 expression under both normoxic and hypoxic conditions. Cardiomyocytes knocked out of both Rab11A, and Rad11B lost the TRAP-induced PAR1 expression but still exhibited the early endosomal TRAP-induced PAR1 expression under hypoxia.

Conclusions
TRAP-mediated activation of PAR1 in cardiomyocytes did not alter the total PAR1 expression under normoxic conditions. Instead, it triggers a redistribution of PAR1 levels under normoxic and hypoxic conditions. TRAP reverses the hypoxia-inhibited PAR1 expression in cardiomyocytes by downregulating Rab11A expression and upregulating Rab11B expression.
Establishing a hyperacute stroke service during the COVID-19 pandemic: our institution’s one year experience
Authors: Abdul Rashid Anna Misya’il , Md Noh Mohamad Syafeeq Faeez , Yusof Khan Abdul Hanif Khan , Loh Wei Chao , Baharin Janudin , Ibrahim Azliza , Ishak Fadhilah Hani , Sardi Aminuddin , Hanapai Ahmad Firdaus , Mohamad Nur Afiqah , Inche Mat Liyana Najwa , Hoo Fan Kee , Wan Sulaiman Wan Aliaa , Basri Hamidon 
Published: February 2023

Abstract
Background and aims
The corononavirus 2019 (COVID-19) pandemic resulted in modifications in the workflow and redistribution of human resources, causing challenges in setting up of an acute stroke service. We would like to share our preliminary outcome amid this pandemic, to determine if the implementation of COVID-19 standard operating procedures (SOPs) affected the delivery of our hyperacute stroke service.

Methods
We retrospectively analyzed one-year data from our stroke registry that began with the establishment of our hyperacute stroke service at Universiti Putra Malaysia Teaching Hospital from April 2020 until May 2021.

Results
Setting up acute stroke services during the pandemic with constrained manpower and implementation of COVID-19 SOPs, was challenging. There was a significant dip of stroke admission from April to June 2020 due to the Movement Control Order (MCO) implemented by the government to curb the spread of COVID-19. However, the numbers of stroke admission steadily rose approaching 2021, after the implementation of recovery MCO. We managed to treat 75 patients with hyperacute stroke interventions i.e. intravenous thrombolysis (IVT), mechanical thrombectomy (MT) or both. Despite implementing COVID-19 SOPs and using magnetic resonance imaging (MRI) as our first line acute stroke imaging modality, clinical outcomes in our cohort were encouraging; almost 40% of patients who underwent hyperacute stroke treatment had early neurological recovery (ENR), and only 33% of patients had early neurological stability (ENS). In addition, we were able to maintain our door-to-imaging (DTI) and door-to-needle (DTN) time in line with international recommendations.

Conclusions
Our data reflects that COVID-19 SOPs did not deter successful delivery of hyperacute stroke services in our center. However, bigger and multi center studies are required to support our findings.
In-stent restenosis and stented-territory infarction after carotid and vertebrobasilar artery stenting
Authors: Ryu Jae-Chan , Bae Jae-Han , Ha Sang Hee , Kwon Boseong , Song Yunsun , Lee Deok Hee , Chang Jun Young , Kang Dong-Wha , Kwon Sun U. , Kim Jong S. , Kim Bum Joon 
Published: February 2023

Abstract
Background
Prognosis after vertebrobasilar stenting (VBS) may differ from that after carotid artery stenting (CAS). Here, we directly compared the incidence and predictors of in-stent restenosis and stented-territory infarction after VBS and compared them with those of CAS.

Methods
We enrolled patients who underwent VBS or CAS. Clinical variables and procedure-related factors were obtained. During the 3 years of follow-up, in-stent restenosis and infarction were investigated in each group. In-stent restenosis was defined as reduction in the lumen diameter > 50% compared with that after stenting. Factors associated with the occurrence of in-stent restenosis and stented-territory infarction in VBS and CAS were compared.

Results
Among 417 stent insertions (93 VBS and 324 CAS), there was no statistical difference in in-stent restenosis between VBS and CAS (12.9% vs. 6.8%, P = 0.092). However, stented-territory infarction was more frequently observed in VBS than in CAS (22.6% vs. 10.8%; P = 0.006), especially a month after stent insertion. HbA1c level, clopidogrel resistance, and multiple stents in VBS and young age in CAS increased the risk of in-stent restenosis. Diabetes (3.82 [1.24–11.7]) and multiple stents (22.4 [2.4–206.4]) were associated with stented-territory infarction in VBS. However, in-stent restenosis (odds ratio: 15.1, 95% confidence interval: 3.17–72.2) was associated with stented-territory infarction in CAS.

Conclusions
Stented-territory infarction occurred more frequently in VBS, especially after the periprocedural period. In-stent restenosis was associated with stented-territory infarction after CAS, but not in VBS. The mechanism of stented-territory infarction after VBS may be different from that after CAS.
Dishevelled 2 regulates cancer cell proliferation and T cell mediated immunity in HER2-positive breast cancer
Authors: Rasha Fahmida , Boligala Geetha Priya , Yang Mingxiao V. , Martinez-Marin Dalia , Castro-Piedras Isabel , Furr Kathryn , Snitman Annie , Khan Sonia Y. , Brandi Luis , Castro Maribel , Khan Hafiz , Jahan Nusrat , Almodovar Sharilyn , Melkus Michael W. , Pruitt Kevin , Layeequr Rahman Rakhshanda 
Published: February 2023

Abstract
Background
Dishevelled paralogs (DVL1, 2, 3) are key mediators of Wnt pathway playing a role in constitutive oncogenic signaling influencing the tumor microenvironment. While previous studies showed correlation of β-catenin with T cell gene expression, little is known about the role of DVL2 in modulating tumor immunity. This study aimed to uncover the novel interaction between DVL2 and HER2-positive (HER2+) breast cancer (BC) in regulating tumor immunity and disease progression.

Methods
DVL2 loss of function studies were performed with or without a clinically approved HER2 inhibitor, Neratinib in two different HER2+ BC cell lines. We analyzed RNA (RT-qPCR) and protein (western blot) expression of classic Wnt markers and performed cell proliferation and cell cycle analyses by live cell imaging and flow cytometry, respectively. A pilot study in 24 HER2+ BC patients was performed to dissect the role of DVL2 in tumor immunity. Retrospective chart review on patient records and banked tissue histology were performed. Data were analyzed in SPSS (version 25) and GraphPad Prism (version 7) at a significance p < 0.05.

Results
DVL2 regulates the transcription of immune modulatory genes involved in antigen presentation and T cell maintenance. DVL2 loss of function down regulated mRNA expression of Wnt target genes involved in cell proliferation, migration, invasion in HER2+ BC cell lines (±Neratinib). Similarly, live cell proliferation and cell cycle analyses reveal that DVL2 knockdown (±Neratinib) resulted in reduced proliferation, higher growth arrest (G1), limited mitosis (G2/M) compared to non-targeted control in one of the two cell lines used. Analyses on patient tissues who received neoadjuvant chemotherapy (n = 14) further demonstrate that higher DVL2 expression at baseline biopsy pose a significant negative correlation with % CD8α levels (r = − 0.67, p < 0.05) while have a positive correlation with NLR (r = 0.58, p < 0.05), where high NLR denotes worse cancer prognosis. These results from our pilot study reveal interesting roles of DVL2 proteins in regulating tumor immune microenvironment and clinical predictors of survival in HER2+ BC.

Conclusion
Our study demonstrates potential immune regulatory role of DVL2 proteins in HER2+ BC. More in-depth mechanistic studies of DVL paralogs and their influence on anti-tumor immunity may provide insight into DVLs as potential therapeutic targets benefiting BC patients.