bluuebunny/crossref_metadata_2025_split huggingface dataset:
An AIE‐active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells
Authors: Wang Fei , Shi Yupeng , Ho Po‐Yu , Zhao Engui , Kam Chuen , Zhang Qiang , Zhao Xin , Pan Yue , Chen Sijie 
Published: May 2023

AbstractPhotodynamic therapy is becoming increasingly popular for combat of bacteria. In the clinical photodynamic combat of bacteria, one critical issue is to avoid the potential damage to the host since the reactive oxygen species produced by photosensitizers are also harmful to mammalian cells. In this work, we report an aggregation‐induced‐emission‐active bacterial inhibitor and photosensitizer, OEO‐TPE‐MEM (OTM), for the imaging, killing, and light‐enhanced inactivation of bacteria. OTM could efficiently bind to and kill Gram‐positive bacteria, while its affinity to Gram‐negative bacteria is lower, and a higher OTM concentration is required for killing Gram‐negative bacteria. OTM is also an efficient photosensitizer and could efficiently sensitize the production of reactive oxygen species, which enhances its killing effect on both Gram‐positive and Gram‐negative bacteria. More interestingly, OTM is very biocompatible with normal mammalian cells both in the dark and under light irradiation. OTM in mice models with bacteria‐infected wounds could promote the healing of infected wounds without affecting their organs and blood parameters, which makes it an excellent candidate for clinical applications.
The implications of the anatomy of the nerves and vessels in the treatment of rectosigmoid endometriosis
Authors: Centini Gabriele , Labanca Luca , Giorgi Matteo , Martire Francesco Giuseppe , Catania Francesco , Zupi Errico , Lazzeri Lucia 
Published: May 2023

AbstractEndometriosis is a common benign gynecological disease characterized by the presence of endometrial glands and stroma outside the uterus. It can be defined as endometrioma, superficial peritoneal endometriosis or deep infiltrating endometriosis (DIE) depending on the location and the depth of infiltration of the organs. In 5%–12% of cases, DIE affects the digestive tract, frequently involving the distal part of the sigmoid colon and rectum. Surgery is generally recommended in cases of obstructive symptoms and in cases with pain that is non‐responsive to medical treatment. Selection of the most optimal surgical technique for the treatment of bowel endometriosis must consider different variables, including the number of lesions, eventual multifocal lesions, as well as length, width and grade of infiltration into the bowel wall. Except for some major and widely accepted indications regarding bowel resection, established international guidelines are not clear on when to employ a more conservative approach like rectal shaving or discoid resection, and when, instead, to opt for bowel resection. Damage to the pelvic autonomic nervous system may be avoided by detection of the middle rectal artery, where its relationship with female pelvic nerve fibers allows its use as an anatomical landmark. To reduce the risk of potential vascular and nervous complications related to bowel resection, a less invasive approach such as shaving or discoid resection can be considered as potential treatment options. Additionally, the middle rectal artery can be used as a reference point in cases of upper bowel resection, where a trans mesorectal technique should be preferred to prevent devascularization and denervation of the bowel segments not affected by the disease.
Improved diagnosis of colorectal cancer using combined biomarkers including Fusobacterium nucleatum, fecal occult blood, transferrin, CEA, CA19‐9, gender, and age
Authors: Zhao Ran , Xia Dongge , Chen Yingwei , Kai Zhentian , Ruan Fangying , Xia Chaoran , Gong Jingkai , Wu Jun , Wang Xueliang 
Published: May 2023

AbstractBackgroundConventional blood and stool tests are normally used for early screening of colorectal cancer (CRC) but the accuracy and efficiency remain to be improved. Recent findings suggest Fusobacterium nucleatum to be a biomarker for CRC. This study evaluated the role of F. nucleatum and developed CRC diagnostic models by combining F. nucleatum with fecal occult blood (FOB), transferrin (TRF), carcinoembryonic antigen (CEA), carbohydrate antigen 19‐9 (CA19‐9), gender, and age.Materials and MethodsCandidates including 71 healthy individuals and 59 CRC patients were recruited. Abundance of F. nucleatum in stool or tissue samples was measured by quantitative real‐time PCR. CEA, CA19‐9, TRF, and FOB were measured in parallel. These biomarkers together with genders and ages were the seven parameters used to develop CRC diagnostic models. Ten different machine learning algorithms were tested to achieve the best performance.ResultsFecal F. nucleatum abundance was found significantly higher in CRC group compared to healthy group (p = 0.0005). Among the CRC patients, F. nucleatum abundance in tumor tissue was significantly higher than that in paracancerous tissue (p = 0.0087). CRC diagnostic models using different parameters were generated based on Logistic Regression algorithm, which showed good performance. The area under the curve (AUC) score of fecal F. nucleatum as the single diagnostic biomarker was 0.68 while the accuracy was 0.56. The diagnostic performance was obviously improved with the highest AUC (0.93) and accuracy (0.87) achieved when using all the 7 clinical parameters. The combination F. nucleatum + FOB + gender + age had the second highest AUC (0.92) and accuracy (0.85). A more utilitarian model using F. nucleatum + FOB showed relatively high AUC at 0.86 and accuracy at 0.81.ConclusionsF. nucleatum is valuable for CRC diagnosis. Combination of different clinical parameters could significantly improve CRC diagnostic performance. The combination F. nucleatum + FOB + gender + age may be an effective and noninvasive method for clinical application.
Synthesis, Biological Evaluation andin SilicoStudies of 3‐Hydroxy‐N‐(2‐(substituted phenyl)‐4‐oxothiazolidin‐3‐yl)‐2‐napthamide Derivatives
Authors: Kamboj Shikha , Thakral Samridhi , Kumar Sunil , Singh Vikramjeet 
Published: May 2023

AbstractIn the present study, a series of 3‐hydroxy‐N‐(2‐(substituted phenyl)‐4‐oxothiazolidin‐3‐yl)‐2‐napthamide derivatives were synthesized, characterized and evaluated for theirin vitroactivity, i. e., antimicrobial, antioxidant and anti‐inflammatory. The target compounds were synthesized by condensation reaction of 3‐hydroxy‐2‐naphthoic acid hydrazide with substituted benzaldehydes which were subjected to cyclization reaction with thioglycolic acid and ZnCl2to get target compounds. The synthesized 3‐hydroxy‐N‐(2‐(substituted phenyl)‐4‐oxothiazolidin‐3‐yl)‐2‐napthamide derivatives were examined for their antimicrobial activity and 3‐hydroxy‐N‐(4‐oxo‐2‐(3,4,5‐trimethoxyphenyl)thiazolidin‐3‐yl)‐2‐naphthamide (S20) exhibited the highest antimicrobial potential. The N′‐(2,3‐dichlorobenzylidene)‐3‐hydroxy‐2‐naphthohydrazide (S5) displayed good antifungal potential againstRhizopus oryzae, whereas N′‐(2,3‐dichlorobenzylidene)‐3‐hydroxy‐2‐naphthohydrazide (S20) showed the highest antioxidant potential and N‐(2‐(2,6‐dichlorophenyl)‐4‐oxothiazolidin‐3‐yl)‐3‐hydroxy‐2‐naphthamide (S16) displayed the highest anti‐inflammatory activity. The results of molecular docking studies revealed that existence of hydrogen bonding and hydrophobic interactions with their respective proteins.In silicoADMET studies were carried out by Molinspiration, Pre‐ADMET and OSIRIS property explorer to predict the pharmacokinetic behaviour of synthesized 3‐hydroxy‐N‐(2‐(substituted phenyl)‐4‐oxothiazolidin‐3‐yl)‐2‐napthamide derivatives.
Study on Calceolarioside A Anti‐Aβ25–35‐Induced Damage in SH‐SY5Y cells by Atomic Force Microscopy
Authors: Chen Yujuan , Qu Kaige , Wang Ying , Zhang Qinghan , Xie Ying , Wang Zuobing 
Published: May 2023

AbstractCalceolarioside A is a phenylethyl glycoside compound, originally isolated from the bark of Fraxinus mandshurica Rupr. In this work, the protective effect of Calceolarioside A on beta Amyloid protein induced toxicity in SH‐SY5Y cells was studied. DPPH experiment, MTT assay, SEM, Atomic force microscopy and Colony formation were used to study the activity of Calceolarioside A. The results in this article show that the survival rate of the cells with Calceolarioside A (20–40 mg/mL) was significantly higher than that of the model cells without Calceolarioside A. Calceolarioside A could protect SH‐SY5Y cells by improving some parameters in cells, such as the cell height, Young's modulus, adhesion and branch. In summary, Calceolarioside A can reduce Aβ25–35‐induced damage in SH‐SY5Y cells. It can be a potential medicine to treatment with AD.
Eosinophilic, polymorphic, and pruritic eruption associated with radiation therapy in two patients diagnosed with prostate cancer
Authors: Barbu Maria Alexandra , Anghel Rodica Maricela , Ricu Gabriel , Tebeica Tiberiu , Patru Raluca , Michire Alexandru 
Published: May 2023

Key Clinical MessageWe report 2 cases of EPPER diagnosed in patients who received radiation therapy and hormonal therapy for locally advanced prostate cancer. Both our patients developed this rare late toxicity, but early diagnosis and treatment of this adverse event offers a good prognosis, with no unnecessary interruptions of oncological treatment required.AbstractAcute and late adverse events are a major problem for patients receiving radiation therapy. We describe two cases of eosinophilic, polymorphic, and pruritic eruption associated with radiotherapy (EPPER) syndrome, a very uncommon toxicity that affects cancer patients. Both our cases were men diagnosed with localized prostate cancer and were treated with radiotherapy and hormonal therapy. They developed EPPER during and after completing the total radiation dose. Multiple tests and skin biopsies were performed in order to find a superficial perivascular lymphohistiocytic infiltrate, confirming EPPER. The patients received corticotherapy and fully recovered after this treatment. There are a few more cases of EPPER reported in the literature, but the pathogenic mechanism is still unknown. EPPER is an important side effect of radiation therapy and it is probably underdiagnosed, due to its occurrence (usually after completing the oncological treatment).
Apexification of dens evaginatus in a mandibular premolar: A case report
Authors: van der Vyver Peet J. , Vorster Martin , Buchanan Glynn Dale 
Published: May 2023

Key Clinical MessageDens evaginatus is an uncommon dental anomaly presenting on mandibular premolar teeth. Affected teeth are difficult to diagnose and manage, and often demonstrate immature apices requiring complex endodontic treatment approaches.AbstractDens evaginatus (DE) is an uncommon anomaly affecting mandibular premolars, frequently requiring endodontic intervention. This report documents the treatment of an immature mandibular premolar presenting with DE. Early diagnosis and preventive strategies remain the preferred management for these anomalies, however endodontic approaches may be successfully applied to retain these teeth.
Granular parakeratosis involving the dorsum of the hands; skin reactions due to irritant agents: A case report
Authors: Shariat Nia Mohammad , Azhari Vahideh Sadat , Shakoei Safoura 
Published: May 2023

Key Clinical MessageWe described the first case of granular parakeratosis with an unusual presentation of brown discoloration plaques and multiple erythematous on the dorsal part of the patient's hands. Skin maceration and repeated washing could have led to the development of the lesions.AbstractGranular parakeratosis is a unique acquired keratinization disorder. Here, we described the abnormal presentation of granular parakeratosis. A healthy female aged 27 years old presented brown discoloration plaques and multiple erythematous on the dorsal part of her hands for 8 months. Using detergents, repeated washing, and skin maceration were considered the causes of her lesion.
Flexible and Binder‐Free Iron Phosphide Electrodes Using a Three‐Dimensional Support for High Hydrogen Productivity
Authors: Díez‐García María Isabel , García Blanco Andrés Alberto , Murcia‐López Sebastian , Botifoll Marc , Arbiol Jordi , Qamar Mohammad , Morante Joan Ramon 
Published: May 2023

AbstractIn this work, an inexpensive and reliable microstructured electrode for the hydrogen evolution reaction (HER) is developed. This cathode is made of Earth‐abundant elements consisting of iron phosphide as an electrocatalyst and carbon felt (CF) as a flexible conductive scaffold. Its porous character and binder‐free FeP coverage over the carbon fibers generate a high number of accessible active sites for the reaction, achieving a high value of the electrochemically active surface area. The electrode reaches 100 mA ⋅ cm−2 by applying only −53 mV vs RHE at 50 °C in 0.5 M H2SO4, demonstrating excellent electrocatalytic activity for the HER and outstanding stability in acidic electrolyte. Furthermore, the feasibility of these electrodes for industrial application is evaluated using a PEM electrolyzer. The developed prototype with a cathodic area of 1.8 cm2 shows a very promising performance, reaching 14.9 mmol H2 ⋅ h−1 ⋅ cm−2 (corresponding to 800 mA ⋅ cm−2) at a voltage of only 2.1 V.
Chiral perturbation on single benzene‐based fluorophores: A structure/(chir)optical activity relationship study
Authors: Coehlo Max , Thuéry Pierre , Pieters Grégory 
Published: May 2023

AbstractIn this paper, we describe the synthesis and the (chir)optical properties of a novel series of circularly polarized luminescent emitters. These molecules involve a compact single benzene‐based donor‐acceptor fluorophore composed of two cyclic alkylamines as electron donors and a phthalonitrile moiety as electron acceptor linked to a configurationally stable BINOL acting as a chiral perturbation unit. These new compounds display fair quantum yields (up to 66%) with emission maxima around 500 nm in toluene solutions, and the study of their chiroptical properties has shown that the cyclic alkylamine's ring size affects significantly the luminescence dissymmetry factors, reaching 2.2 × 10−3 for the larger cyclic alkylamine moieties.
Suadimins D—J, Monoterpenoid Indole‐Quinoline and Bisindole Alkaloids from Melodinus suaveolens
Authors: Gao Xin‐Hua , Wu Pei‐Qian , Fan Yao‐Yue , Zhou Bin , Yue Jian‐Min 
Published: May 2023

Comprehensive SummaryCompounds 1—12, including seven complex monoterpenoid indole‐quinoline and bisindole alkaloids, suadimins D—J (1—7) with previously unreported carbon‐carbon linkages, were isolated and identified from the twigs and leaves of Melodinus suaveolens. Their structures were elucidated by a combination of diverse methods, especially the extensive spectroscopic data analysis and electric circular dichroism (ECD) calculations. The cytotoxicity of these compounds against three cancer cell lines was evaluated, some of which showed moderate activities with IC50 values ranging from 2.4 to 8.0 μM.
Mitotic rate in primary cutaneous melanoma: Cell division matters
Authors: Gershenwald Jeffrey E. 
Published: May 2023

The prognostic significance of primary cutaneous melanoma tumor mitotic rate (TMR) has been of worldwide clinical interest for decades. Is there a path forward to build on the contributions from the report by Patuzzo et al. and other studies to refine the classification and staging of melanoma and to develop clinical tools to improve risk and prognostic assessment that include TMR to inform decision‐making?
MiR‐548 K regulatory effect on the ABCG2 gene expression in MDR breast cancer cells
Authors: Saberiyan Mohammadreza , Ghasemi Zahra , Yaghoobi Hajar 
Published: May 2023

AbstractBackgroundmultidrug resistance (MDR) is One of the foremost challenges in overcoming breast cancer. Various molecular processes are involved in the development of MDR in breast cancer cells, including over expression of ABC transporters such as ABCG2 (BCRP), increase breast cancer stem cells drug resistance, and epithelial mesenchymal transition.AimsIn the present study, we used bioinformatics and experimental analysis to investigate the role of miR‐548 K, in the modulating of ABCG2, in MDR breast cancer cells.Methods and ResultsIn silico inspections introduce 14 microRNAs targeting 3′‐UTR region of ABCG2 transcripts, which are probably involved in breast cancer drug resistance. An association was highlighted between miR‐548 k with ABC transporter family. The expression level of ABCG2 gene in MCF7‐MX cell lines was significantly more than MCF7 cell lines. On the other hand, we increased the expression of miR‐548 K in MCF7‐MX and MCF7 cell lines through its transfection, which dramatically coincided with decreasion in the ABCG2 transcripts level. Additional studies on patient samples revealed that the expression of ABCG2 showed an increase in ABCG2 level in neoadjuvant chemotherapy drugs resistance (NCDR) patients compared to primary pre‐operative chemotherapy drugs response (PCDR) patients. Also, a reduction in the expression of miR‐548 K in NCDR patients was revealed.ConclusionThe results of our study suggest that miR‐548 K may be involved in modulating the expression of ABCG2 in MDR breast cancer cells.
Anti‐PD‐1 monoclonal antibody‐resistant esophageal squamous cell carcinoma showing the abscopal effect: A case report with T‐cell receptor/B‐cell receptor repertoire analysis
Authors: Takehara Yuka , Mimura Kosaku , Suzuki Yoshiyuki , Watanabe Yohei , Yoshimoto Yuya , Saze Zenichiro , Sato Hisashi , Tamaki Tomoaki , Kono Koji 
Published: May 2023

AbstractBackgroundSeveral clinical trials of nivolumab have reported good results, including those in patients with advanced esophageal squamous cell carcinoma. However, the response rate of this drug remains poor. Notably, a rare phenomenon called abscopal effect refers to the regression of irradiated and nonirradiated distant tumors after local radiotherapy. Although the mechanism of this effect remains unclear, the antitumor immunity induced by radiotherapy is considered to be the most important factor.CaseA 66‐year‐old man with recurrent nivolumab‐resistant esophageal squamous cell carcinoma along with left‐side cervical and abdominal para‐aortic lymph node metastases was treated with a 40 Gy (10 fractions) dose of radiotherapy to the left‐side cervical lymph node metastasis as a palliative treatment, which caused neck pain. In addition, nivolumab administration was resumed the day after completion of radiotherapy. Three months after radiotherapy, the irradiated lesion on the left neck had regressed to a scar‐like lesion. Furthermore, the previously progressive abdominal para‐aortic lymph nodes outside the irradiation area shrank (abscopal effect). T‐cell receptor and B‐cell receptor (TCR/BCR) repertoire analyses before and after radiotherapy revealed that radiotherapy led to changes in the TCR/BCR repertoire.ConclusionChanges in the TCR/BCR repertoire may be a part of the mechanism underlying the abscopal effect. The findings of the present case suggest that the combination of immune checkpoint inhibitors and radiotherapy is a promising treatment approach even for patients with immune checkpoint inhibitor‐resistant cancer.
Single‐Cell Glycan and RNA Sequencing (scGR‐seq)
Authors: Odaka Haruki , Tateno Hiroaki 
Published: May 2023

AbstractSingle‐cell multi‐omics analysis has emerged as a crucial tool in modern biology due to its capacity to uncover the intricate molecular heterogeneity within individual cells. Glycan structural diversity on the cell surface creates a "cell signature" that varies by cell type and state. However, single‐cell glycan analysis remains a challenge. This protocol presents a series of techniques and procedures that enable the simultaneous measurements of glycan and RNA at the single‐cell level via DNA‐barcoded lectin‐based sequencing (scGR‐seq). The techniques include (1) the preparation of DNA‐barcoded lectins, (2) a step‐by‐step protocol for single‐cell glycan/RNA sequencing, and (3) a data analysis approach for the integration of glycan and RNA data. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Preparation of DNA‐barcoded lectinsBasic Protocol 2: Single‐cell glycan/RNA sequencing
Influence of Cr2O3 Concentration on the Green Color of Flux Synthesized Emeralds Using the CIE1976 Color System
Authors: Kozaki Daisuke , Yanagisawa Kazumichi , Oishi Shuji , Nishiwaki Yoshinori , Komatsu Hibiki , Kobiro Kazuya , Ito Akitaka , Taniguchi Ayano , Matsuzaki Takuya 
Published: May 2023

AbstractThe color of emeralds is predominantly influenced by the concentration of Cr3+. However, the evaluation of the trend of green color depth of emeralds, based on the practical single crystal synthesis with several instrumental analysis is not investigated in previous researches. Specifically, if the Cr2O3 content is controlled to be in the range of 0.2–0.4 wt%, then emeralds can be synthesized with the most esthetically pleasing color. Therefore, the effect of Cr2O3 concentration within the aforementioned range on the color of emeralds is studied using powder X‐ray diffractometry, digital microscopy, electron probe microanalysis, and micro‐spectrophotometry to understand how to achieve the most esthetically pleasing color. As a result, detailed behaviors relating to the absorbances, L*, a*, and b* values, and the relationship between the emerald crystal color changes and increases in Cr2O3 concentration are elucidated. Notably, the absorbance, L* and a* values change significantly when the Cr2O3 concentration is low (0.00–0.236 wt%). However, the changes in these values are moderate when the Cr2O3 concentration is higher (0.236–0.504 wt%).
A reinforcement learning‐based energy management strategy for a battery–ultracapacitor electric vehicle considering temperature effects
Authors: Wang Chun , Liu Rui , Tang Aihua , Zhang Zhigang , Liu Pu 
Published: May 2023

SummaryThe design of energy management strategy (EMS) plays a vital role in the power performance and economy of battery–ultracapacitor for electric vehicles. A reinforcement learning (RL)‐based EMS is proposed to obtain an optimal power allocation strategy for battery–ultracapacitor electric vehicle, and its robustness is verified at different temperatures. First of all, the dynamic characteristic experiments of the battery and ultracapacitor were performed at 10°C, 25°C, and 40°C to obtain mechanism characteristics at different temperatures. Secondly, a genetic algorithm is selected to identify the parameters of the battery and ultracapacitor model. Next, the RL‐based strategy takes the minimum energy loss of the hybrid energy storage system as the reward function and solves the optimal policy based on Markov theory. The simulation results show that the economy of the RL‐based strategy correspondingly improved by 3.05%, 3.20%, and 3.15% at different temperatures in comparison with the fuzzy‐based strategy, and the economic gap between the RL‐based strategy and the DP‐based strategy is further narrowed down to 7.30%, 3.88%, and 8.40% at different temperatures, respectively. Finally, the proposed strategy is validated under different driving conditions, which indicate that the RL‐based strategy can effectively reduce energy consumption and has good robustness at different temperatures.