bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of the Microbial Quality of Food Contact Surfaces (Utensils) of Hotels and Restaurants in Addis Ababa
Authors: Tenna Abiyot , Amare Kassayenew , Tekola Hiwot , Kidan Yohannes W. , Melese Daniel , Medhin Girmaye 
Published: February 2023

Abstract

Background: Food contact surfaces are surfaces that encounter food for humans. It can harbor and introduce pathogens into food. Objective: The aim of this study was to assess the microbiological quality of food contact surfaces (utensils) from hotels and restaurants in Addis Ababa. Methods: We used cross-sectional study design in which 12 hotels and 28 restaurants were randomly selected in Addis Ababa. Swab samples from utensils of hotels and restaurants were collected (i.e. four utensils from each establishment) and analyzed over the period of three months resulting in a total of 160 swab samples. Result: Among the hotel and restaurant utensils the highest median count of log total coliform was obtained on trays (5.93log10CFU/100cm2in hotels and 5.00log10CFU/100 cm2, in restaurants). Faecal coliform andE.coliwas detected in 14.37% and 3.12% of utensils, respectively. The highest median count of logS.aureuswas 5.95log10CFU/100cm2on tray in hotel and 5.57log10CFU/100cm2on dipper in restaurant and, the median counts of log APC was 9.37log10CFU/100cm2on tray in Hotel and 8.51log10CFU/100cm2on spoon in restaurant. Conclusion: The finding showed that there is high microbial load and inadequacy of washing and cleaning services in hotels and restaurants in Addis Ababa. Hence, there is a need to strengthen monitoring and supervision system is hotels and restaurants.
The Iterative Solution Of Taylor Formula For Partial Differential Equation
Authors: GAO Yang 
Published: February 2023

Abstract
This paper discuss the relation between Taylor's formula and partial differential equation. Taylor formula iteration method can resolve partial differential equation. u(x, t)be expanded at t = 0or t = 1by Taylor formula Coefficient of Taylor formula ut(x; 0),utt(x; 0). . . can be expressed by partial differential equation. The method can solve nonlinear differential equation.Generalized Taylor's formula can solve fractional partial differ- ential equation. The method is very important way that resolving partial differential equation. The method also can resolve those equations from [1]-[9]. This article refers to the literature [10]. Taylor formula iteration method belong to logical thinking.
Aliilewinella sediminis gen. nov. sp. nov., isolated from the coastal sediment, and reclassification of some Lewinella species as the members of the genus Alilewinella
Authors: Zhang Ya-Jing , Ji Chu-Xuan , Li Fan , Zhong Yan-Lin , Gong Ya , Du Zong-Jun 
Published: February 2023

Abstract
A Gram-negative, non-motile, aerobic, gliding, slender rod-shaped bacterium was isolated from the coastal sediment of Xiaoshi Island, Weihai, China. The newly isolated strain, designated W8T, grew at 15–37°C (optimum, 30°C) and pH 6.0–8.5 (optimum, pH 7.0–7.5) in the presence of 1.0–5.0% (w/v) NaCl (optimum, 3.0%). The major cellular fatty acids were summed feature 3 (C16:1ω7c/C16:1ω6c), iso-C15:0, iso-C17:0 3-OH, and summed feature 9 (C16:0 10-methyl/iso-C17:1ω9c). The sole isoprenoid quinone of the strain was MK-7. The polar lipid profile consisted of one phosphatidylethanolamine, one unidentified phospholipid, and eight unidentified polar lipids. Based on 16S rRNA gene sequence similarity, strain W8T was found to be closely related to Lewinella agarilytica KCTC 12774T (94.5%), L. lacunae KCTC 42187T (94.1%) and L. aurantiaca SSH13T (93.3%), belonging to the family Lewinellaceae. Based on the results of polyphasic taxonomic analyses and genomic analyses, strain W8T (= KCTC 72084T = MCCC 1H00378T) is considered to represent a novel species within a new genus Aliilewinella, for which the name Aliilewinella sediminis gen. nov., sp. nov. is proposed. We also propose to reclassify 11 species of the genus Lewinella as the members of the genus Aliilewinella.
Androgen-induced exosomal miR-379-5p release determines granulosa cell fate: The cellular mechanism of anovulatory polycystic ovaries
Authors: Salehi Reza , Wyse Brandon A. , Asare-Werehene Meshach , Esfandiarinezhad Fereshteh , Abedini Atefeh , Pan Bo , Urata Yoko , Gutsol Alex , Vinas Jose L. , Jahangiri Sahar , Xue Kai , Xue Yunping , Burns Kevin D. , Vanderhyden Barbara , Li Julang , Osuga Yutaka , Burger Dylan , Tan Seang-Lin , Librach Clifford L. , Tsang Benjamin K. 
Published: February 2023

Abstract

Polycystic ovarian syndrome (PCOS) is a complex multi-factorial syndrome associated with androgen excess and anovulatory infertility. In the current study, we investigated the role of dihydrotestosterone-induced exosomal miR-379-5p release in determining the destiny of the developing follicles. Our hypothesis was that androgen regulates granulosa cell miR-379-5p content by facilitating its exosomal release in a follicular-stage dependent manner, a process which determines granulosa cell fate. Compared to human non-PCOS subjects, individuals with PCOS exhibit higher follicular fluid free testosterone levels, lower exosomal miR-379-5p content and granulosa cell proliferation. Androgenized rats exhibited lower granulosa cell miR-379-5p but higher phosphoinositide-dependent kinase-1 (PDK1; a miR-379-5p target) content and proliferation. Androgen reduced granulosa cell miR-379-5p content by increasing its exosomal release in preantral follicles, but not in antral follicles in vitro. Studies with an exosomal release inhibitor confirmed that androgen-induced exosomal miR-379-5p release decreased granulosa cell miR-379-5p content and proliferation. Ovarian overexpression of miR-379-5p suppressed granulosa cell proliferation, and basal and androgen-induced preantral follicle growth in vivo. These findings suggest that increased exosomal miR-379-5p release in granulosa cells is a proliferative response to androgenic stimulation specific for the preantral stage of follicle development and that dysregulation of this response at the antral stage is associated with follicular growth arrest, as observed in human PCOS.
Unveiling structure transformation and activity origin of the heteroatom-doped carbons for hydrogen evolution
Authors: Lu Shanshan , Cheng Chuanqi , Shi Yanmei , Zhang Zhipu , Zhang Bin 
Published: February 2023

Abstract

Heteroatom-doped carbon materials have been widely used in many aqueous electrocatalytic reduction reactions. Their structure-activity relationships are mainly explored based on the assumption that the doped carbon materials keep stable during electrocatalysis. Thus, the structural evolutions of heteroatom-doped carbon materials are often ignored, and their active origins are still unclear. Herein, taking N-doped graphite flake (N-GP) as the research model, we present the hydrogenation of both N and C atoms and the consequent reconstruction of the carbon skeleton during hydrogen evolution reaction (HER), accompanied by a remarkable promotion of the HER activity. The N dopants are gradually hydrogenated and almost completely dissolved in the form of ammonia. Theoretical simulations demonstrate that the hydrogenation of the N species leads to the reconstruction of the carbon skeleton from a hexagonal to a 5,7-topological ring (G5-7) with thermoneutral hydrogen adsorption and easy water dissociation. P-, S-, and Se-doped graphite also shows the similar removal of doped heteroatoms and the formation of G5-7 rings. Our work unveils the activity origin of heteroatom-doped carbon toward the HER and opens a door to the rethinking structure-performance relationships of carbon-based materials for other electrocatalytic reduction reactions.
Planning and design of forest park rehabilitation base oriented to an artificial intelligence algorithm
Authors: Zhu Zhaoling , Ma Huaquan 
Published: February 2023

Abstract
A water ecosystem is a dynamic balance system with a certain structure and function, which is composed of aquatic organism community and water environment interaction and mutual restriction through material circulation and energy flow. Living in an urban environment for a long time can make people suffer from ‘urban disease’, which can seriously affect their physical and mental health. Forest rehabilitation is a new form of outdoor activity in many countries, which has been gradually promoted by society in recent years. Many studies have confirmed that forest environments have positive impacts on the human body and mind, so forest healthcare and treatment are crucial to the prevention and treatment of ‘urban diseases’. This study is based on artificial intelligence (AI) to carry out the planning and design of the forest health base. According to sensory design theories including air factor, somatosensory factor, and audiovisual factor, this paper proposed different landscape configuration methods to enhance health and well-being. Finally, it was found that in the planning and design of the health base, when the human comfort index is greater than 85 and less than 20, it is very uncomfortable for the human body.
Cardiospecific Troponins as Laboratory Biomarkers of Myocardial
Cell Injury in Hypertension: A Mini-Review
Authors: Chaulin Aleksey Michailovich 
Published: February 2023

Abstract:
To date, it is well known that a significant number of diseases of cardiovascular
genesis (coronary heart disease, myocardial infarction, cardiomyopathy, Takotsubo
syndrome, heart failure, etc.) and extra-cardiac genesis (renal failure, chronic obstructive
pulmonary disease, sepsis, diabetes mellitus, etc.) cause injury to contractile cells of the
heart muscle (myocardial cells). The most sensitive and specific criteria for proving myocardial
cell injury are cardiospecific troponins (CSTns) - CSTnI and CSTnT. According
to the current clinical recommendations of the European, American, and Russian Cardiological
Communities, CSTnI and CSTnT are the main biomarkers for early diagnosis of
myocardial infarction. Hypertension is one of the most dangerous and common risk factors
for the development of cardiovascular pathologies and is associated with a high risk
of dangerous cardiovascular complications. Therefore, there is an urgent need to search
for new biomarkers for the timely assessment of the prognosis of patients with hypertension.

:
This mini-review aims to substantiate the possibilities of using the cardiomarkers (CSTnI
and CSTnT) to assess the prognosis of patients suffering from hypertension and to discuss
potential mechanisms that cause injury to myocardial cells and increase serum levels
of CSTnI and CSTnT.

:
This is a narrative mini-review, which was prepared using the following databases:
Pubmed/Medline, PubMed Central, Embase, Scopus, and Web of Science. The following
keywords were used in the literature search: “myocardial cells”, “injury”, “damage”, and
“hypertension” in combination with the terms “mechanisms of injury” “predictive significance”,
“cardiac troponins”, or “cardiospecific troponins”.
A Scoping Review on Recent Progress on Purpurin and its Derivatives
Authors: Rustage Kajol , Chauhan Pragati , Kapoor Kapish , Kumar Manish , Baniya Bhuvanesh , Jain Vivek 
Published: February 2023

Background:
Purpurin is being used as a red dye for many decades. But recently, due to its
pharmacological properties, purpurin and its derivatives have attracted a lot of researchers for the treatment
of various ailments, such as cancer, Alzheimer’s disease, depression, etc.

Objectives:
The objective of this study is to provide an overview of its pharmacological properties, pharmacokinetic
studies, synthesis, isolation, quality assurance, and patent studies.

Method:
A systemic scoping review was undertaken. Three databases (Pubmed, Scopus, and Google
Scholar) and patent websites were searched using relevant words (e.g., purpurin, purpurin derivatives,
anticancer, toxicity, etc.). All outcomes for studies that met the inclusion criteria were included in the
review. Extracted data were accumulated using tables, figures, and accompanying narrative descriptive
summaries. The review was reported using the preferred reporting items for scoping review (PRISMAScR)
guidelines. Sixty-eight studies and eighty-six patents met the inclusion criteria, mostly preclinical
(in vitro, in vivo, and in silico) studies performed in rats, mice, dogs, and zebrafish, followed by one clinical
trial study.

Results:
The potent antioxidant nature of purpurin is the main reason behind its vast pharmacological
properties. It acts by decreasing mitochondrial stress and by acting on the endoplasmic reticulum. It also
crosses the BBB barrier, has high GI absorption, and follows the Lipinski rule, which makes it a good
drug for various neurodegenerative disorders. It inhibits various CYP-450, CYP 1A2, and CYP 3A4 enzymes,
which are responsible for causing mutations. It gets photosensitized by UV light and causes ROSdependent
apoptosis in cancer cells.

Conclusion:
This scoping review highlights purpurin and its derivatives as highly prized moieties in the
treatment of various neurological conditions and cancer. The unique nature of purpurin is responsible for
its pharmacological properties, which are due to the presence of hydroxyl and keto groups at specific
positions. It gets photosensitized by UV and laser light and acts as an anticancer drug. But the lack of
robust evaluation in clinical studies is another major concern. Purpurin can be seen in the prescription in
the future, although a lot of work still needs to be done.
Is Tinospora cordifolia Responsible for Drug-induced Liver Injury?
Authors: Singh Preet Amol , Bajwa Neha 
Published: February 2023

Abstract:
A number of journal articles written by researchers claiming Tinospora cordifolia
(Giloy) is to blame for liver damage have sparked a wide range of debates between Ayush and the
allopathic medical community, particularly in India. Some even argue that promoting Giloy-based
formulations to the general public is a bad idea. In this backdrop, the purpose of this essay is to
provide a counter-narrative based on some of the major gaps in the research that implicated Giloy
in Drug-induced Liver Injury (DILI). Time-tested herb, Giloy, which has been used for pharmacological
benefits since antiquity, as well as clinical and toxicological evidence suggest that Giloy is
safe, and the observed negative effects can be attributed to Tinospora crispa, a herb that resembles
Tinospora cordifolia in appearance. Holding Giloy species to be detrimental is unjustifiable unless
other variables such as authentication, standardization, Good Agricultural and Collection Practices
(GACP), Good Manufacturing Practices (GMP), regularization of the market are considered. This
article emphasizes the importance of strategic collaboration between plants and contemporary
medicine in order to eliminate concerns about the use of Giloy by the general public.
JDHY3 Inhibits Hypopharyngeal Carcinoma Cell Proliferation and Promotes Apoptosis
by Inhibiting the PI3K/AKT Pathway
Authors: Dai Weili , Ni Zhen , Zhang Ganlin , Xu Jia , Qin Xiaoyan , Cao Jingpeng , Liu Liangfa 
Published: February 2023

Background:
Jieduhuayu No.3 (JDHY3) is a modified Chinese herbal formula beneficial for treating hypopharyngeal
carcinoma (HC), but its pharmacological mechanism is unknown.

Objective:
This study aimed to explore the mechanism of the herbal formula JDHY3 in inhibiting cell proliferation and
promoting apoptosis in HC in vitro and in vivo.

Methods:
In this study, HC cells were treated with cisplatin and different concentrations of JDHY3. The apoptosis rate
was detected by flow cytometry. Western blotting was used to detect the proteins related to cell proliferation and apoptosis.
Afterward, the xenograft mouse model was established and treated with cisplatin and JDHY3. Mouse tumour
volume was measured, and the tumour tissues were assessed by HE staining and immunohistochemistry.

Results:
JDHY3 significantly inhibited the proliferation of FaDu and Detroit-562 cells. In addition, JDHY3 significantly
increased the apoptosis rate of HC cells and downregulated p-PI3K and p-Akt. In addition, JDHY3 upregulated
the expression of the apoptosis-promoting proteins Bax, P53, and cleaved caspase-3. In addition, the expression of the
antiapoptotic protein Bcl-2 was downregulated. Coincubation with SC79 attenuated the decrease in cell proliferation
induced by JDHY3, further confirming that the proapoptotic effect of JDHY3 is associated with the inhibition of
PI3K/Akt pathway activation.

Conclusions:
The results of in vivo experiments showed that JDHY3 could effectively inhibit the proliferation of HC
cells, and HE staining showed that JDHY3 reduced the invasion of HC cells. Immunohistochemistry showed that the
expression of P53 and cleaved caspase-3 was significantly increased in the tissues of the JDHY3-treated group.
The Relationship between Insulin Resistance and Thyroid Volume in Georgia
Authors: Lomtadze Nino , Giorgadze Elene , Janjgava Shota , Kacharava Tinatin , Taboridze Iamze 
Published: February 2023

Abstract:
Patients with insulin resistance (IR) have a higher thyroid volume therefore the aim of our study is to examine the correlation between IR and thyroid volume in the residents of Georgia.

Methods:
413 patients with a mean age of 37.3 and 11.4 years were included in this study. Out of those, 120 were males, and 293 were females who were studied retrospectively. They had hyperinsulinemia and were referred to the clinic from 2017 to 2019.
The factors studied were age, sex, body mass index (BMI), clinical signs, thyroid ultrasound, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipids, fasting insulin, fasting glucose, thyroid stimulating hormone (TSH), Free thyroxine (FT4), and Zinc (Zn).

Results:
IR was detected in 252 individuals. The frequency of men with insulin resistance was significantly higher than in the control group - 72.50%, and 56.31%, respectively (F = 9.55, p = 0.0021). Mean thyroid volume in the patients with IR was significantly higher compared to the controls 20.52 + 6.39 cm3 and 15.25 + 6.55 cm3, respectively (p < 0.001). Hyperinsulinemia had a significant positive correlation with Goiter r = 0.445, p < 0.0001. The associated factors for hyperinsulinemia are: Goiter (1) – OR = 5.12 (95% CI:3.02-8.69); Cholesterol – OR = OR = 3.31 (95% CI: 1.54-7.14); Triglycerides – OR = 3.23 (95% CI:1.02-10.28); Obesity (1)- OR = 3.94 (95% CI: 2.23-6.98); Thyroid structural changes (1) – OR = 2.01 (95% CI: 1.12-3.60); ALT/AST-OR = 4.53 (95% CI: 2.33-8.80); Zn decreased Odds Ratio hyperinsulinemia – OR = 0.95 (95% CI: 0.94-0.97).

Conclusion:
Hyperinsulinemia is the most common cause of diffuse goiter and the heterogeneous structure of the thyroid. The volume of the thyroid gland shows a significant positive association with the characteristics of metabolic syndrome and increased thyroid volume predictors of metabolic syndrome.
Protective Effect of Chrysin against Chlorpyrifos-Induced Metabolic
Impairment and Pancreatitis in Male Rats
Authors: Samarghandian Saeed , Farkhondeh Tahereh , Naseri Kobra , Safarzadeh Mahdieh , Moghaddam Mahdieh Rajabi , Aramjoo Hamed , Roshanravan Babak 
Published: February 2023

Background::
This study was performed to evaluate the protective effects of chrysin (CH) on metabolic impairment and pancreatic injury caused by sub-chronic chlorpyrifos (CPF) intoxication in male rats.

METHODS:
Forty male Wistar rats were randomly allocated into five groups (n=8). Intraperitoneal injections of chrysin (12.5, 25 and 50 mg/kg for 45 days) and CPF (10 mg/kg for 45 days) gavage were performed. Present findings indicated that the serum levels of glucose, total cholesterol, and low-density lipoprotein-cholesterol, as well as body weight, were increased in the CPF-exposed group.

Results::
It was also found that CPF decreased superoxide dismutase activity as well as increased malondialdehyde and nitric oxide levels in the pancreatic
tissue of exposed animals. Histopathological examination also confirmed the toxic effects of CPF on pancreatic tissue as mostly evidenced by
infiltration of inflammatory cells and necrosis. CH (50 mg/kg) decreased blood glucose concentration (p < 0.05), TG (p < 0.05), and LDL-C in
CPF-exposed animals. CH decreased the pancreas levels of MDA in all treated CPF-exposed groups versus the non-treated CPF-exposed group (p
< 0.05, p < 0.001, p < 0.001, respectively). A significant difference was not seen in the NO and MDA levels and SOD activity between CH-treated
(50 mg/kg) animals exposed to CPF and controls. A significant difference was not seen in the NO and MDA levels and SOD activity between CHtreated
(50 mg/kg) animals exposed to CPF and controls.

Conclusion::
A significant difference was not seen in the NO and MDA levels and SOD activity between CH-treated (50 mg/kg) animals exposed to CPF and controls. In conclusion, CH could prevent initiate and progress of CPF-induced metabolic impairment by modulating oxidative stress in pancreatic tissue as a target organ of organophosphorus pesticides.
Potential Perspectives and Sustainability of Bioplastics Developed from
Horticulture
Authors: Saini Pinki , Iqbal Unaiza , Ahmed Mazia 
Published: February 2023

Abstract:
In recent times, bioplastics have become an integrated sustainable alternative to plastic
management to lessen the dependency on fossil fuels as well as better plastic disposal methods.
Through this study, the focus is laid upon the dire need for developing bio-plastics for transforming
to a sustainable future as bio-plastics are renewable, more feasible, and a sustainable option
when compared to the high-energy consuming conventional oil-based plastics. Bioplastics
might not be a one-stop solution for all environmental issues caused by plastics, but it will be a
beneficial step for expanding biodegradable polymer as society’s current concerns about the environment
makes this an ideal time for further growth of biopolymers. Moreover, the potential
market for agricultural materials in bioplastics is leading to an economic push toward the growth
of the bioplastic industry, thus providing better alternatives for a future sustainable environment.
The objective of the review is to provide detailed knowledge about plastics obtained from various
renewable sources, their production, life cycle, market share, applications, and roles to act as
a sustainable source of synthetic plastics, thereby featuring various possibilities and potentialities
of bioplastics to perform as an alternative solution for waste reduction.