bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the impact of vine pruning periods on grape production and composition: an integrated approach considering different years and cultivars
Authors: Ghiglieno Isabella , Facciano Luca , Valenti Leonardo , Amari Federico , Cola Gabriele 
Published: January 2025

Several studies have already highlighted how late pruning, conducted after bud break, can result in a delay in phenology and, consequently, in ripening. However, the effects of pruning periods on vegetative and productive parameters are less well known. The aim of this study was to evaluate the effect of pruning periods on ripening kinetics, grape composition at harvest and yield parameters, considering the grapevine cultivar and year. The experimentation was conducted from 2011 to 2013 in two of the main Italian wine-growing areas: Franciacorta, in Northern Italy, and Montefalco, in Central Italy. In Franciacorta: Chardonnay and Pinot Noir were considered while in Montefalco, the varieties included were Sauvignon Blanc, Grechetto di Orvieto, Sagrantino, and Sangiovese. Six different pruning periods were compared: at leaf fall (NOV), during dormancy (DEC, JAN, FEB), in the pre-bud break period (MAR) and late, post-bud break (APR). Results obtained showed that the effects of the pruning period are clear and sufficiently replicable only considering pre-bud brake pruning (APR). The observation of the ripening kinetics highlighted in fact a delay in grape ripening for APR in both contexts as well as a lower values for the sugar-acidity ratio at harvest. In terms of grape yield, a moderately negative effect of APR was observed in Montefalco, while a more severe effect was observed in Franciacorta. This analysis gives a comprehensive understanding of the effects of pruning timing on grape yield and composition, providing viticulturists with transversally applicable information, useful for an aware management of the vineyard.
Human Papillomavirus and Cervical Cancer Screening
Authors: Pérez-Gracia María Teresa , Tarín-Pelló Antonio , Fernández-Álvarez Sara , Más-Comes Clara , Suay-García Beatriz 
Published: January 2025

Human papillomavirus (HPV) is responsible for the most common sexually transmitted infection worldwide. Cervical cancer (CC) is a severe disease that still has a high mortality rate in many countries. Persistent infection by the human papillomavirus is the necessary cause of premalignant lesions, which are precursors of CC. Screening with cytology or Pap smear have been successful in the last few years, but scientific evidence suggests that these techniques should be replaced with HPV DNA detection as the initial test when screening. There are several commercial techniques approved by the FDA for the detection of high oncological risk HPV genotypes, which have been evaluated for the population screening of 30-65-year-old women. The aim of this article is to review some virologic aspects of HPV and its relationship with cervical cancer screening. Consequently, we suggest that a new algorithm based on individualized detection of genotypes 16 and 18 should be implemented in countries where there is no population-based screening.
SYNCHRONIZATION OF GnRH AND PGF2Α ON THE REPRODUCTIVE PERFORMANCE IN ACEH CATTLE
Authors: Melia Juli , Desita Ella , Khalifah Hadid , Justika Wulan , Armansyah Teuku , Hasan Muhammad , Rosmaidar Rosmaidar , Sayuti Arman , Aliza Dwinna , Wahyuni Sri 
Published: January 2025

This study aims to determine the effect of administering gonadotropin-releasing hormone (GnRH) accompanied by prostaglandin F2 alpha (PGF2α) on the reproductive performance of female Aceh cattle. In this study, eight female Aceh cattle were used, divided into two groups, group I (K1, n= 3) which received estrus synchronization treatment with PGF2α and group II (K2, n= 5) which received estrus synchronization treatment with PGF2α (25 mg/cow) and gonadotropin-releasing hormone (GnRH, 100 µg/cow). GnRH injection in K2 was performed 48 hours after PGF2α injection. The estrus displays measured included onset, duration, and intensity of estrus. Blood collection for estrogen examination was taken from day 1 to day 5 after PGF2α injection, while progesterone examination was taken on days 7, 14, and 21 after artificial insemination. Ovarian response and pregnancy examinations were performed using transrectal ultrasonography (USG). Data on estrus appearance and steroid concentration were analyzed using the Mann-Whitney U test, and differences in pregnancy percentage were analyzed using the chi-square test. In contrast, the number of follicles, dominant follicle diameter, and corpus luteum were analyzed using a split-plot test. Oestrus intensity, oestrus onset, oestrus duration, pregnancy percentage, estrogen and progesterone concentrations, ovarian response in class II follicles, class III follicles, and CL size in K1 vs K2 showed no significant differences (P0.05). The effect of time on ovarian response in class III follicles and CL size showed a significant difference (P0.05). In contrast, the interaction between hormone administration and day of treatment on ovarian response in class II follicles, class III follicles, and CL size showed no significant difference (P0.05). Based on the research results, it was concluded that administration of GnRH accompanied by PGF2α tends to increase the reproductive performance of Aceh cattle.
Identification of Colorectal Adenocarcinoma Key Genes to Be Validated as Potential Prognosis Biomarkers
Authors: Mahgoub Elham Omer , Mankar Pooja Prakash , kumar Amit , Naqv Syed Asif , Mittal Bharti 
Published: January 2025

Abstract

Background: Colorectal Adenocarcinoma (COAD). continues to be one of the leading causes of death worldwide. The patient's chance of survival increases with the early prognosis of a malignant tumor.
Methods: Advanced bioinformatics methods were used to gain a thorough understanding of the genetic landscape of colorectal cancer. The transcriptome RNA-seq raw data were obtained from PRJEB24758 in the European Nucleotide Archive (ENA) database. Then, the Sequence Read Archive (SRA) runs a selection to download the sequences database. The transcriptomic RNA released data is analyzed by the bioinformatics tool, which also uses online analytic tools to help visualize the results and identify key genes that may be employed as prognosis biomarkers in the future. The annotation pathways have been determined using David's annotation tools, and cluster analysis in Gsea and the c-bioportal database also showed the significance of these pathways. Two hundred fifty miRNA overlapped with the highest two upregulated and downregulated genes that were subjected to screening. The Venn diagram determined the common genes that the immunogenic genes set and cell type gene signature.
Results: 1,274 genes with substantial differential expression in colorectal cancer were found using stringent approaches such as HISAT2 alignment and DeSeq2 analysis. This study identified 913 upregulated genes of colorectal adenocarcinoma (COAD). The upregulated genes-expressed profile of COAD was studied. The upregulated genes are controlled by 20 pathways expressed in colorectal adenocarcinoma. David's annotation tool was used to prepare diagrams for the KEGG analysis, enriched genes, and many more diagrams. The resulting miRNA overlapped genes interacted significantly with TF to produce key genes. From a different perspective, fifty-seven upregulated common genes were determined using the Venn diagram, and higher mutated genes were selected. Investigating these key genes for targeted therapy in colorectal cancer therapy is crucial, as the study emphasizes.
Conclusion: Thus, the development of novel therapeutic approaches and the identification of key genes of the changed expression of genes implicated in COAD drug resistance are crucial goals for the ongoing advancement of COAD therapy.
Facet-selective electrostatic assembling of 2D MXene onto anisotropic single-crystal metal oxides for enhanced photocatalysis
Authors: Kashiwaya Shun , Myakala Stephen , Nekita Sho , Tsuji Yuta , Niu Yuran , Xianjie Liu , Qin Leiqiang , Kakharov Alexei , Hultman Lars , Dominik Eder , Saito Hikaru , Cherevan Alexey , Rosen Johanna 
Published: January 2025

Abstract

This work demonstrates an electrostatic assembly strategy for the facet-selective deposition of two-dimensional (2D) transition metal carbides (MXenes) onto anisotropic single-crystal semiconducting metal oxides. By precisely controlling the solution pH, we modulated the surface charge of the MXenes and the distinct crystallographic facets of the metal oxides, enabling selective deposition driven by electrostatic attraction. Specifically, negatively charged Mo4/3C MXenes were selectively deposited on the electron-rich (101) surface of TiO2 exposed with {101} and {001} facets at pH 3, the (100) surface of Cu2O, exposed with {100} and {111} facets at pH 11, and the (010) surface of BiVO4, exposed with {010} and {110} facets at pH 1.5. The high degree of facet selectivity was confirmed through a combination of advanced techniques, including electron microscopy, electron spectroscopy, and synchrotron-based spectromicroscopy. This selective interfacial engineering promotes spatially separated charge carrier migration towards distinct facets of the oxides, while Schottky barriers form at the MXenes/oxides interfaces, further enhancing charge separation. The MXenes act as efficient reduction co-catalysts, facilitating the rapid consumption of electrons trapped at the Schottky barriers, thereby enhancing photocatalytic hydrogen evolution.
MR neurography of tibial and common peroneal nerves in patients with Guillain-Barre syndrome and electrophysiological correlation
Authors: Cao Jinfeng , Wang Shanshan , Gong Tao , Zheng Wenjing , Sun Peng , Wang Guangbin 
Published: January 2025

Abstract

Background
The early and accurate evaluation of peripheral nerve injury in Guillain–Barre syndrome (GBS) patients is of great significance for clinical diagnosis and treatment. There has been recent interest in using Diffusion-weighted imaging with background suppression (DWIBS) to evaluate peripheral nerves and corresponding lesions, but has not been applied in GBS patients.
Objective
To explore the value of MR neurography in evaluating tibial nerve (TN) and common peroneal nerve (CPN) in GBS patients.
Methods
36 GBS patients and 36 healthy volunteers were included in this prospective study. The cross-sectional areas (CSA) and signal-to-noise ratio (SNR) values of TN and CPN on T2-weighted images were calculated. Four-grade scoring system was used to score DWIBS images of TN and CPN. The CSA and SNR values, nerve scores on DWIBS were compared. Pearson correlation tests were used to assess the correlation between the CSA and SNR values, nerve scores and electrophysiology parameters of the GBS group.
Results
The interobserver agreement of measurements and nerve scored values was excellent. The mean CSA and SNR values of TN and CPN were significantly larger in patients than healthy controls (P<0.05). There were statistically significant differences in nerve scores between two groups (P < 0.01). The SNR values of TN correlated negatively with motor nerve conduction velocity (MCV) and motor nerve conduction amplitude (P < 0.01). The SNR values of CPN correlated negatively with MCV (P = 0.02). The nerve scores of TN and CPN were all positively correlated with MCV and motor nerve conduction amplitude (P < 0.01).
Conclusions
MR neurography showed larger CSA, higher SNR values of TN and CPN and unclear nerve on DWIBS. The SNR values and nerve scores on DWIBS have correlation with electrophysiological parameters. These findings suggest that MR neurography can be useful to assess the damage of TN and CPN in GBS patients.
Whole-exome sequencing screening for candidate genes and potential pathogenic variants associated with early-onset high myopia in 47 Chinese families
Authors: Rui Xue , Li Huiping , Ma Runqing , Yang Shangying , lian Yuanyuan , Cheng Wanyu , Ma Meijiao , Rong Weining , Sheng Xunlun 
Published: January 2025

Abstract

Early-onset high myopia (eoHM) occurs before school age and is an ideal model for monogenic studies of high myopia due to minimal environmental influence. This study screened for genes and variants associated with eoHM in 47 unrelated Chinese patients with eoHM. Protein-protein interaction (PPI) network analysis was conducted to detect interactions among candidate genes, and protein-protein docking was performed. In 28 patients (28/47, 59.6%), 32 potential pathogenic variants in 22 candidate genes were identified, including 24 novel variants. Among these 28 patients, 64.3% (18/28) carried pathogenic variants in RetNet genes. Of these, 12 patients (42.9%, 12/28) had pathogenic variants in six known genes (TSPAN12, CACNA1F, USH2A, RPGR, COL2A1, and COL11A1), which are responsible for retinal dystrophy and Stickler syndrome associated with eoHM. Additionally, 7 patients (25.0%, 7/28) carried pathogenic variants in seven candidate genes for ocular disease (POLA1, TMEM231, HK1, GSN, COL5A1, CRYBB3, and WDR), which were identified as potentially pathogenic in Chinese eoHM patients for the first time. Phenotype analysis showed that 11 patients presented with only high myopia, 10 patients had inherited retinal diseases (IRDs) with eoHM, and 7 patients had ocular-only Stickler syndrome (Ocular-STL) with eoHM. The initial clinical records of these 17 patients did not show recognizable signs of other primary diseases except for high myopia, and further specific clinical examinations confirmed the diagnosis of IRDs or Stickler syndrome through eoHM. The PPI network analysis identified 87 candidate genes associated with early-onset high myopia (eoHM), grouped into four functional clusters. Thirteen hub genes, including RPGR, COL5A1, CRYAB, and FBN1, were crucial for the pathogenesis of myopia. The network showed strong biological relevance with highly significant enrichment (p-value < 1.0e-16). Our study expands the list of candidate genes associated with eoHM and suggests that eoHM may be the first reason for children to visit an ophthalmology clinic and an important clue for clinicians to detect underlying ocular diseases. These findings highlight the complex interplay of these genes, providing valuable insights into the molecular mechanisms of myopia and potential targets for future therapeutic interventions.
Identification of Key Genes in Colorectal Cancer Associated with Primary Sclerosing Cholangitis and Inflammatory Bowel Disease
Authors: Yang Jingbang , Deng Yizhang , Chen Na , Lin Dagui , Zhao Enen , Zhang Linjie , Li Liren 
Published: January 2025

Abstract

Inflammatory bowel disease (IBD) and primary sclerosing cholangitis (PSC) are both gastrointestinal inflammatory diseases that exhibit a significant correlation with an elevated incidence of colorectal cancer (CRC). However, the mechanisms that contribute to the increased risk of CRC in patients suffering from IBD and PSC remain poorly understood. In this study, two distinct modules were identified within the PSC and IBD cohorts by using WGCNA. Following the intersection of 243 genes from these modules, 21 prognosis-related factors were screened through univariate Cox regression analysis. An unsupervised consensus clustering algorithm was then applied, leading to the identification of two subtypes. Survival analysis revealed that cluster 2 exhibited a poorer prognosis. Subsequently, LASSO and multivariate Cox regression analyses were conducted to identify 5 critical genes from the prognosis-related factors, thereby establishing a prognostic risk model. Higher risk score correlated with a poorer prognosis. A nomogram was constructed based on the risk model and clinical factors. Its prognostic prediction ability was validated through calibration curves and decision curve analysis (DCA), demonstrating good accuracy. Survival analysis indicated that FLOT1 was strongly correlated with poor prognosis in both cohorts, and its role in promoting tumor growth and invasion in CRC was verified in vitro.
Analysis of high-risk factors and prognosis of early relapse in 104 patients with natural killer/T-cell lymphoma: a single-center retrospective study
Authors: Zhang Mengjuan , Li Xin , Li Xinyue , Shi Chunzhen , Wu Xiaolong , Zhang MingZhi 
Published: January 2025

Abstract

Objective:To investigate the risk factors for early recurrence in natural killer/T-cell lymphoma(NKTCL) patients following anti-tumor therapy, and to assess the effects of various treatment approaches on the prognosis of NKTCL patients.
Methods: Retrospective analysis was performed on 104 newly treated NKTCL patients. The patients were categorized into two groups:POD24 and non-POD24, based on whether the disease progressed after the first 24 months of treatment or not.
Results: The POD24 group's 3-year and 5-year overall survival(OS) rates were 47.63% and 30.99%, respectively, while the non-POD24 group's rates were 63.99% and 44.58%. Elevated lactate dehydrogenase(LDH)(P=0.036), PET-CT SuvMax≥21.0(P=0.021) and failure to achieve complete response(CR) at the end of treatment(P=0.017) were associated with the early recurrence of NKTCL. The DDGP group had higher 5-year OS(P=0.048) and PFS(P=0.044) rates than the other groups(SMILE, P-Gemox, VIDP, DICE, etc.).The CR rate of radiotherapy plus chemotherapy was significantly higher than that of chemotherapy alone(P < 0.001).
Conclusion: POD24 can serve as a prognostic indicator for identifying the survival of NKTCL patients, aiding in the formulation of optimal treatment strategies in clinical practice. Clinically, high-risk early relapse patients can be identified early through general clinical characteristics, allowing for intervention to improve disease prognosis. The DDGP regimen can demonstrate better long-term efficacy in newly treated NKTCL patients, while the combination of radiotherapy and chemotherapy can provide better short-term efficacy for localized NKTCL patients.
Implementation of School Improvement Program in Secondary Schools in Assosa zone
Authors: Mesfin Shimelis 
Published: January 2025

Abstract

Purpose: The purpose of this study was to assess the implementation of school improvement program (SIP) in secondary schools of Assosa zone.
Methods: Descriptive survey design was employed. The size of the population was 316 which consisted of 302 teachers and 14 school principals. Of these, 177 respondents were taken into the sample using stratified random sampling technique. Close ended questionnaire and interview were used to collect the data. Descriptive and inferential statistical analysis techniques were used to analyze the data collected through the questionnaire.
Results: The result of one sample t-test showed that teachers and school principals had low perception on the awareness, planning and implementation of SIP. The result of independent sample t-test revealed that statistically significant mean differences were not found between teachers and school principals on awareness, planning, and implementation. The result of one way ANOVA indicated that there was no significant difference among respondents on awareness of SIP due to their work experience, while had different perception on the planning, and implementation of SIP. SIP was poorly implemented due to lack of awareness, resources, skilled manpower, collaboration, smooth relationships; follow up on SIP and absence of self-evaluation.
Conclusion: Based on the finding of this study, it is concluded that SIP is poorly implemented due to personal and institutional factors.
Prevalence and Risk Factors associated with Malaria infection in Children under five years in Northern Uganda.
Authors: Kula Jaspher , Opoke Robert 
Published: January 2025

Abstract

Background: Malaria still remains the leading cause of morbidity and mortality among children in sub-Saharan Africa with over 95% of children in Uganda particularly still suffering from it. About US$9 is annually lost in malaria treatment which is estimated at 3% of the family annual income. Malaria remains a prominent public health burden despite several attempts in recent years to control its spread. This study ascertained the prevalence and associated risk factors of malaria infection in children below 5 years old.
Methods: A cross-sectional study was done in two randomly selected villages in Otangocinge parish, Arwotcek sub county on children under five years screened for malaria from Arwotcek Health Centre II from December 2019 to October 2020 to ascertain the risk factors associated with malaria infection and their mothers or caretakers were interviewed to ascertain the risk factors of malaria infection in Amolatar District. Prevalence of malaria in children under five years old was calculated as a proportion of the malaria positive cases versus negative cases at 95% CI (confidence interval). Bivariate analyses were conducted by determining the associations between prevalence of malaria and its associated risk factors using Chi-square (χ2) test followed by binary logistic regression taking into consideration p-values < 0.05 as significant factors using the SPSS software version 20.
Results: Of the 121 children screened for malaria, 57 (47.1%) tested positive for malaria while 64(52.9%) children tested negative using the Rapid Diagnostic Tests (RDTs). Income status of the family (p<0.001), proximity of homesteads to water sources (p<0.001), insecticide treated mosquito nets usage and its physical conditions (p<0.001) and age of the child (p= 0.002) and the location of the homestead (p= 0.042) are the potential risk factors associated with malaria transmission.
Conclusion: Although many efforts have been done to calm the burden of malaria, the government should empower the village health teams(VHTs) in providing basic malaria diagnosis and community sensitization about the risk factors of malaria, regular distribution of mosquito nets and timely indoor residual spraying.
Current Scenario in Associating Clinical COVID-19 Biomarkers for Developing Surveillance Platforms
Authors: Lakshmipriya Thangavel , Gopinath Subash C.B. , Chen Yeng , Sasidharan Sreenivasan , Subramaniam Sreeramanan , Gunny Ahmad Anas Nagoor , Md Ali Umi Fazara , Salimi M.N. , Fakhri Makram A. , Salim Evan T. , Thirugnanasambandan Theivasanthi 
Published: January 2025

Abstract:
The novel coronavirus that caused the epidemic and pandemic resulting in the
acute respiratory illness known as coronavirus disease 2019 (COVID-19) has plagued
the world. This is unlike other coronavirus outbreaks that have occurred in the past, such
as Middle East respiratory syndrome (MERS) or severe acute respiratory syndrome
(SARS). COVID-19 has spread more quickly and posed special challenges due to the
lack of appropriate treatments and vaccines. Real-time polymerase chain reaction (RTPCR)
and rapid antibody tests (surveillance tests) are the two most used tests (confirmation
tests). However, the latter takes hours to complete, and the former may produce
false positives. Scientists have invested significant effort to create a COVID-19 diagnostic
system that is both highly sensitive and reasonably priced. Early detection of
COVID-19 is a major area of focus for sensing devices based on nanomaterials. This
overview enhanced insights into potential coronavirus biomarkers and, compared to earlier
studies, introduced new avenues. Further, it covers the development of COVID-19
diagnostic systems from an analytical point of view, including clinical markers and their
subsequent applications with biosensors.