bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lumican promotes proliferation, migration and invasion of gastric cancer through ERK pathway
Authors: liu Guangxi , Li Xin , Shen Xiaobing 
Published: March 2023

Abstract
Purpose
To clarify the molecular mechanism of lumican's effects on gastric cancer cell proliferation, migration, and invasion.
Methods
qRT-PCR was used to analyze lumican expression in gastric cancer tissues and cell lines. Small interfering RNA (siRNA) transfection and lentivirus infection have been used to produce lumican knockdown or overexpression gastric cancer cell models from screened cell lines. CCK-8, wound healing assays, and transwell assays were performed to confirm the effect of lumican on gastric cancer cell’s proliferation, migration, and invasion. To further evaluate the potencial mechnism of lumican on gastric cancer cells, bioinformatic prediction and western blot experiment were used to identify and confirm its related signaling pathway.
Results
Using MGC-803 and AGS gastric cancer cells, lumican knockdown or overexpression was achieved. Overexpression of lumican increased MGC-803 and AGS gastric cancer cell proliferation, migration, and invasion, whereas knockdown decreased them. The expression levels of ERK and p-ERK, two key proteins of the ERK pathway, were significantly decreased in MGC-803 and AGS cells with lumican knockdown, while the opposite result was observed with lumican overexpression; the expression levels of MEK and p-MEK, two key proteins of the MEK pathway, were not significantly changed with lumican knockdown or overexpression. GDC-0994, an ERK pathway inhibitor, restored ERK1/2 and p-ERK1/2 protein expression in MGC-803 and AGS cells overexpressing lumican.
Conclusion
Lumican was discovered in high levels in the tissues of patients with gastric cancer, and it promoted proliferation, migration, and invasion in gastric cancer cells. Lumican may impact the expression of two main proteins, ERK1/2 and p-ERK1/2, in the ERK signaling pathway rather than activating it via MEK, indicating that the pathway may be a therapeutic target for lumican overexpression in gastric cancer.
Highly Accurate Memristor Modelling Using Mos Transistor for Analog Applications
Authors: Soni K. , Sahoo Satyajeet 
Published: March 2023

Abstract

Memristor technology has grown at a breakneck pace over the last decade, with the promise to transform data processing and storage. A memristor is a non-linear electrical component with two terminals that connects electric charge and magnetic flux. The ability to store and process data in the same physical location is a fundamental benefit of memristors over traditional electrical components. It has a unique feature in that its resistance may be preset (resistor function) and then saved (memory function). Memristors, unlike other types of memory used in modern electronics, are stable and retain their state even if the device is turned off. In this work, a new highly accurate asymmetrical memristor is proposed for highly efficient analog applications. The proposed work used 5 Complementary metal-oxide-semiconductor (CMOS) devices in a parallel and series-connected manner. A bypass transistor is used to control the current flow between two terminals to perform a stable operation. A differential amplifier circuit is used to validate the proposed memristor performance. The proposed work is implemented using TSMC 45nm CMOS technology. This application consumes less power and has good performance when compared with conventional techniques. In this work, a 1V power supply occupies a 67.5 µm2 layout area. The experimental results are improved when compared with the existing circuit.
Systemic Practice in the Business Mountain Models nexus Internet of Things
Authors: Covaci Brindusa , Covaci Mihai 
Published: March 2023

Abstract
The paper proposes an aggregate index for systemic practice regarding mountain business development through the Internet of Things (IoT) and Information and Communication Technology (ICT). The paper presents an aggregate index, build from unequal indices, which sustain systemic practice of the business development in the mountain area, especially through ICT and IoT. The proposed model is predictive. The paper contains relevant data related to the Eurostat index Business Demography and activities of ICT and IoT for some European mountain areas. Data have been processed and simulated in Excel, SPSS, and Make fractal software. The article performed exploratory and quantitative analysis, using statistics and forecasting techniques based on ANOVA. The indicator, the first in the specific literature and its application, develop considerably systemic practice of the mountain business in Europe, in the 2022 context, declared by the United Nations as the ”International Year of Sustainable Mountain Development”.
From the consumers’ side: Determining students’ perception and intention to use ChatGPT in Ghanaian higher education
Authors: Bonsu Emmanuel Mensah , Baffour-Koduah Daniel 
Published: March 2023

Abstract

The advent and development of technologies such as artificial intelligence have created benefits and challenges for educational stakeholders. Artificial intelligence, such as OpenAI’s Chat Generative Pre-trained Transformer (ChatGPT), has brought new benefits, challenges and pressures for higher educational institutions. There are relatively few reports of how students perceive and intend to use ChatGPT. Taking cognizance of these, the research explored students’ perceptions and intentions to use ChatGPT in their higher education. Guided by two hypotheses and a mixed-method approach, the analysis revealed no statistical relationship between perception and intention to use ChatGPT. Despite this statistical relationship, the study reported students’ positive perceptions towards ChatGPT, and intentions to use ChatGPT and advocated its adoption in education. Based on these findings, the study offers implications for educational practices and further research.
Physical aspects of chemical carcinogenesis
Authors: A.P Batyanov 
Published: March 2023

Abstract
This study investigated the effects of chemical carcinogenesis induced by 7,12-dimethylbenz(α)anthracene (DMBA) in mice. The respiratory activity of isolated mitochondria in mouse livers was investigated at various times after the subcutaneous administration of DMBA. Our findings indicated that DMBA derivatives did not exhibit damaging effects on mitochondrial membranes. On the contrary, they showed a stimulating effect on the phosphorylation process compared with the control. This paper discusses the possible role of coherent electromagnetic radiation in biological systems. Additionally, data on the non-chemical interactions between isolated mitochondria and the material (plexiglass and quartz) of the incubation cells were considered. Carcinogenesis was previously considered an exclusive process of biochemical and genetic disorders.However, recent studies have increasingly focused on the physical aspects of carcinogenesis, such as the influence of various wavelengths of electromagnetic radiation on biological systems, the special energy organization of the intracellular space associated with the architectonics of mitochondria and microtubules, and the generation of coherent electromagnetic waves. It was assumed that the biological features of the malignant cells were due to the violation of the electrodynamic parameters of the intracellular space, and the initiating factor was the intrinsic emission of carcinogenic substances. The results of this study indicated that non-chemical factors influenced the mitochondrial poly-enzyme system and confirmed the importance of the non-chemical effect of a carcinogen on bio-objects by examining respiration and phosphorylation of isolated mouse liver mitochondria at various times after the subcutaneous injection of DMBA.
Plasma transfusions in failure of colostral immunoglobulin transfer (1)
Authors: Anderson K. L. , Hunt E. , Fleming S. A. 
Published: March 2023

Partial or complete failure of colostral immunoglobulin transfer (FCIT) is frequently encountered in neonatal calves and is associated with increased morbidity and mortality. Therapy for FCIT and associated diseases may include administration of antimicrobials, fluids, various supportive measures and attempts to restore protective levels of circulating immunoglobulins (Ig). Because FCIT is frequently encountered after gut closure to absorption of exogenous Ig, administration of Ig in plasma or whole blood has been used to restore protective levels of Ig. Recommendations for plasma administration to neonatal calves are empirical and have been based upon practices commonly used in neonatal foals. A commonly recommended dose is administration of plasma at 20ml/kg body weight.
In the present study, plasma administration was studied in newborn calves with FCIT. The purposes of the study were: 1.) To determine if administration of plasma at 20ml/kg would produce protective levels of circulating Ig in calves with FCIT; and 2.) To compare levels of circulating Ig in calves administered plasma IP once, IV once, and IV twice with 7 days between dosing. In addition, calves with FCIT and administered plasma were compared to calves receiving colostrum.
Effects of slatted flooring on claw shape in intensively housed fattening beef cattle
Authors: Murphy Peter A. , Hannan John 
Published: March 2023

Intensivism in the Irish beef industry is a recent and fast-developing process involving the concentration of cattle into large slatted floor houses where they are confined for a six-month winter period. Surveys carried out to assess any alterations in the disease pattern as a result of changing to slatted floors from traditional straw yard housing indicated that twice as much disease (10% incidence) was encountered on slatted floors as on straw yard (5% of cattle).
Lameness with an incidence of five percent of cattle affected was the most serious disease problem on slats and the major lesion causing lameness was septic traumatic pododermatitis which accounted for 43 percent of all lameness cases. Surveys of defects of claws post-mortem indicated that abrasion of the white line was the primary inciting cause of bruising and penetration at the white line though ungulysis or chronic foot rot was an important secondary cause.
Abrasion of hoof horn was considered a function of two factors—floor construction and horn quality. Accordingly to assess the influence of floor construction on claw shape through abrasion the rates of horn growth and wear were measured in beef cattle housed either on slats or on straw over a six-month winter period.
Case Report
Authors: Weaver A. David 
Published: March 2023

In a review of 129 cases of pelvic hernia due to this cause encountered over a period of 30 years, the majority were right-sided and were corrected by rectal manipulation and rupture of the occluding band. Laparotomy, however has been performed since 1813 and has been claimed to be the preferable method, in particular during the later years of the nineteenth century.
The condition of “gut tie” is described in detail including left flank laparotomy for section of the intra-abdominal strangulating cord by a bistoury.
This form of incarceration appears to be much less common today. In 100 cattle with intestinal obstruction Pearson found that the etiology involved inflammatory lesions caused by traction castration or traumatic lesions of the parietal peritoneum in only four cattle. Adhesions were not present in this current case. Similar obstructions of jejunoileal segments have been caused by a persistent hepatic round ligament, para-ovarian bands and vitello-umbilical bands or by adventitious fibroserosal cords. Differential diagnosis from such abdominal bands was impossible in this case but in retrospect might have been suspected since this particular band is more caudal than other forms reported in the past twenty years. It is doubtful whether it could have been ruptured by digital manipulation per rectum and such manipulation would also have been unnecessarily hazardous to the steer.
Surgical fistula as an aid in the treatment of chronic bloat in cattle
Authors: Anderson J. F. , Frederiksen E. D. 
Published: March 2023

Medical treatment of chronic bloat in cattle often fails, resulting in acute death, no weight gain, or poor weight gain.
Several devices have been developed to facilitate the treatment of chronic bloat. These consist of self-retaining trocars, plastic or metal fistulas, and pop-off valves. Usually these devices become plugged postoperatively, either by local infection of the rumen or by ingesta.
If an animal has been treated medically for bloat on three occasions within a short period of time, the case may be classified as chronic. Surgical intervention is then recommended. Young beef animals that develop chronic bloat soon after they are started on high-concentrate feed are the usual candidates for this procedure.
Diagnosis of respiratory disease in feedlot cattle occurring after being in feedlot for 45 days
Authors: Edwards A. J. 
Published: March 2023

During 1985 eleven feedlots located in Kansas and Nebraska under direct health care programs instituted by the author, received a total of 372,175 cattle. There was a 7% morbidity rate in these cattle and a .65% mortality. Respiratory diseases accounted for 65% of the morbidity and the mortality during this period.
Of the 26,674 cattle treated in these feedlots, 33% of them had been on feed for more than 45 days and of the 2,420 animals that died, 46% of these had been in the feedlot for more than 45 days.
Eversion of the bovine bladder
Authors: Hojbjerg Aage 
Published: March 2023

Most often, prolapse of the bladder (prolapsus vesicae) is observed by rupture of the wall of the vagina in connection with difficult parturition. Due to a short and wide urethra, eversion of the bladder is especially common in the mare, and almost solely related to parturition, characterised by strong contractions. In spite of a longer and more narrow urethra, eversion of the bladder does occur in cows from time to time, but these cases are also solely related to parturition. This fact is obviously caused by contractions related to birth, but also by walls of the urethra relaxing in this period. Paresis of the bladder may also result in a case of eversion.
When everting, the bladder turns inside out and is forced out through the urethra - to be found in the vagina or outside labiae in the form of a prolapse the size of a child's head. Soon after the eversion, changes in the mucosa of the bladder occur, as it turns bluish, thickens, and feels oedematous. At this stage, it is not immediately obvious that the prolapsed part is the bladder, and the diagnosis must be established at a thorough examination. The base of the prolapse, in a case of an everted bladder, can be found in the entrance of or inside the urethra.
From a point of differential diagnosis, the following must be considered: Prolapsed bladder without inversion, vaginal polyps, vaginal prolapse, vaginal tumours, and the fetal membrane bladder.
Oxytocin update for practicing veterinarians
Authors: Reneau Jeffrey K. 
Published: March 2023

From the limited amount of research conducted to date, it appears that injection of moderate doses of oxytocin at each milking will increase milk production. However, there are many unanswered questions about the use of oxytocin in this manner. Perhaps the most important issue is the question of human safety of dairy products potentially containing oxytocin. Until more information on oxytocin is generated and FDA approval for oxytocin use as a production aid is given, it should only be prescribed and used according to its therapeutic intent.
Presented at the Dairy Herd Health Programming Conference, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, June 6-7, 1990.
Application of planned animal health and production to dairy farms
Authors: Esslemont R. J. , Wassell Barbara R. , Grimbleby Linda M. , Wassell Tim R. , Lamb Jackie M. , Horne Shirley 
Published: March 2023

It is estimated that in the UK, on the 100,000 full-time farms, there are nearly 10,000 microcomputers in use. Approximately 2000 of these farms have a dairy recording program on a computer incorporated into the milking parlour or in the office.
The development of dairy recording systems on microcomputers has been taking place for over ten years. The system described (DAISY — The Dairy Informaton System) was developed from a mainframe herd health system in use in 1968 at the University of Melbourne, and developed at the University of Reading (UK) from 1972 onwards. DAISY accepts any type of individual cow record (identity, life events, health, fertility, milk yield and quality etc.,). No records are lost and all entries are thoroughly validated. The user chooses how much detail to enter.
Review of the veterinarian's role in the handling of down/disabled cattle
Authors: Stark Deborah A. 
Published: March 2023

Veterinarians are keenly interested in animal welfare issues and are contributing to the discussions and educational programs at local, provincial and federal levels, but livestock welfare begins at the farm. Besides promoting humane handling of livestock, exactly what should bovine practitioners do to help their clients deal with down cows?
The American Association of Bovine Practitioners (AABP) has stated that bovine practitioners have three key roles in the care and handling of down/disabled animals.They are:• to help prevent conditions leading to ambulatory problems• to provide an early and accurate prognosis• to recommend appropriate disposition alternatives.
Super-normal milk production subsequent to ventral paramedian abomasopexy
Authors: Ehrlich Jim 
Published: March 2023

37 cases of right- and left-sided abomasal displacement were operated and the abomasum was fixed ventrally. The surgical cases were matched with controls in the same herd and the milk yield was compared over a 12-month period before and after surgery. From the 6th to the 12th month after surgery, the surgical cases produced 5-10 lb more milk than the controls. It was suggested that subclinical abomasal displacement is a common cause of low performance in high-yielding dairy cows.
This paper should be viewed as a case study, not a full analysis of a complex disease. The results suggest strongly that there may be positive effects of abomasopexy beyond simply cutting the losses associated with this production disease.
Uniform protocol for evaluating response to treatment of papillomatous digital dermatitis lesions
Authors: Britt Jenks S. , Berry Steven L. , Shearer Jan , Hemling Tom , Steevens Barry , Dreher Monika 
Published: March 2023

Digital dermatitis or papillomatous digital dermatitis (PDD), commonly known as footwarts or hairy heel warts, was first reported in Italy in 197 4 and in New York in the late 1970's. Similar lesions have occurred in 40 or more states in the United States and many other countries. The etiology of this disease is unknown, however, two to five phylotypes of spirochetes (Treponema sp.) have been associated with active lesions and identified by using Polymerase Chain Reaction (PCR), histologic, or microbiological methods.
The purpose of this paper is to develop a uniform protocol which can be used to evaluate results of treatment to control PDD lesions. The remainder of this paper lists our suggestions for information which should be included in each trial protocol.
Comparison of tildipirosin and tulathromycin for control of bovine respiratory disease in high-risk beef heifers
Authors: Compiani Riccardo , Baldi Gianluca , Bonfanti Maurizio , Fucci Daniele , Pisoni Giuliano , Jottini Stefano , Torres Siddartha 
Published: March 2023

The purpose of this study was to compare the effectiveness of tildipirosin (TIP) to tulathromycin (TUL) administered at arrival to reduce morbidity in beef heifers (Charolais; n=785; age=11.1ñ1.9 months; average body weight=830.9 ± 78.48 lb (376.9 ± 35.6 kg)) at high risk of developing bovine respiratory disease (BRD). BRD morbidity was lower in the TIP group (TIP=6.8%; TUL=20.9%; P<0.01) over the feeding period. Animals in the TIP group had greater average daily gain compared to heifers in the TUL group (TIP=2.49 lb (1.13 kg); TUL=2.34 lb (1.06 kg); P<0.01). No differences were observed between groups for number and severity of lung lesions. In the present study, tildipirosin was more effective than tulathromycin in reducing BRD morbidity and improving growth performance in newly received beef heifers considered at high risk for BRD.
Fecal egg count reduction test evaluating macrocyclic lactones using cattle treated 118 days earlier with saline, albendazole in combination with doramectin, or an extended-release formulation of eprinomectin
Authors: Yazwinski T. A. , Tucker C. A. , Powell J. , Beck P. G. , Wray E. G. , Jones L. , Koltes J. E. , Hernandez C. G. 
Published: March 2023

A fecal egg count reduction (FECR) test was conducted on stocker cattle treated 118 days earlier with saline injection (S); 0.09 mg/lb (200 mcg/kg) BW doramectin injection concomitantly with 4.54 mg/lb (10 mg/kg) BW albendazole oral suspension (DA); or 0.45 mg/lb (1 mg/kg) BW eprinomectin extended-release injection (ERE), and then continuously grazed by treatment group in groups of 4 until the start of the current study. In total, there were 8 S, 12 DA, and 12 ERE grazing groups (pastures of origin). Three animals from each pasture were randomly allocated for injection with ivermectin, doramectin or moxidectin, all at the rate of 0.09 mg/lb (200 mcg/kg) BW. Fecal samples were obtained at days -1 and 15, with treatments on day 0. Additionally, coprocultures were conducted on individual fecal samples collected on days -1 and 15.
On day -1 of the FECR test, the arithmetic mean strongyle eggs per gram of feces counts (EPG) across all pastures of origin were 412, 570, and 321 for the ivermectin, doramectin, and moxidectin-treated cattle, respectively. Day 15 egg counts in the same order were 177, 335, and 28. Using the above arithmetic means and by standard equation, the overall mean FECR percentages were 57.0, 41.2, and 91.2 for ivermectin, doramectin, and moxidectin, respectively. Lowest FECR test percentages were seen for animals from the ERE pastures. Coproculture larvae populations harvested on both days -1 and 15 were primarily Haemonchus placei and Cooperia punctata, regardless of animal's pasture of origin or FECR test treatment.
Review of digital anatomy, infectious causes of lameness, and regional intravenous perfusion in cattle
Authors: Simpson Katharine M. , Streeter Robert N. , Jones Meredyth L. , Taylor Jared D. , Callan Robert J. , Holt Timothy N. 
Published: March 2023

Digital disorders in cattle constitute the majority of lameness issues that bovine practitioners will encounter. Treatment of many digital infections in cattle can be facilitated in part by regional limb perfusions of either local anesthetics, antibiotics, or both. This article reviews infectious causes of digital disease, relevant clinical anatomy, use of various perfusates and tourniquet types in regional intravenous perfusions in cattle, and potential complications associated with the procedure.