bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vitamin C deficiency in osteogenic disorder Shionogi/Shi Jcl-od/od rats: effects on sour taste preferences, lick rates, chorda tympani nerve responses, and taste transduction elements
Authors: Yasuo Toshiaki , Nakamura Fumihiko , Suwabe Takeshi , Sako Noritaka 
Published: March 2023

Abstract
Animals use sour taste to avoid spoiled food and to choose foods containing vitamins and minerals. To investigate the response to sour taste substances during vitamin C (ascorbic acid; AA) deficiency, we conducted behavioral, neural, anatomical, and molecular biological experiments with osteogenic disorder Shionogi/Shi Jcl-od/od rats, which lack the ability to synthesize AA. Rats had higher 3 mM citric acid and 10 mM AA preference scores when AA-deficient than when replete. Licking rates for sour taste solutions [AA, citric acid, acetic acid, tartaric acid, and HCl] were significantly increased during AA deficiency relative to pre- and postdeficiency. Chorda tympani nerve recordings were conducted to evaluate organic acid taste responses in the AA-deficient and replete rats. Nerve responses to citric acid, acetic acid, and tartaric acid were significantly diminished in AA-deficient rats relative to replete controls. There was no significant difference in the number of fungiform papillae taste buds per unit area in the AA-deficient rats relative to the replete rats. However, mRNA expression levels of Gnat3 (NM_173139.1), Trpm5 (NM_001191896.1), Tas1r1 (NM_053305.1), Car4 (NM_019174.3), and Gad1 (NM_017007.1) in fungiform papillae taste bud cells from AA-deficient rats were significantly lower than those in replete rats. Our data suggest that AA deficiency decreases avoidance of acids and reduces chorda tympani nerve responses to acids. AA deficiency downregulates some taste-related genes in fungiform papillae taste bud cells. However, the results also reveal that the mRNA expression of some putative sour taste receptors in fungiform papillae taste bud cells is not affected by AA deficiency.
Intact FGF23 predicts serum phosphate improvement after combined nicotinamide and phosphate binder treatment in hemodialysis patients
Authors: Egli-Spichtig Daniela , Hamid Ahmad Kamal , Arroyo Eva Maria Pastor , Ketteler Markus , Wiecek Andrzej , Rosenkranz Alexander R , Pasch Andreas , Lorenz Horst , Hellmann Burkhard , Karus Michael , Ammer Richard , Rubio-Aliaga Isabel , Wagner Carsten A 
Published: March 2023

ABSTRACT

Background
Hyperphosphatemia is associated with increased mortality and cardiovascular morbidity of end-stage kidney failure (ESKF) patients. Managing serum phosphate in ESKF patients is challenging and mostly based on limiting intestinal phosphate absorption with low phosphate diets and phosphate binders (PB). In a multi-centric, double-blinded, placebo-controlled study cohort of maintenance hemodialysis patients with hyperphosphatemia, we demonstrated the efficacy of nicotinamide modified release (NAMR) formulation treatment in addition to standard PB therapy in decreasing serum phosphate. Here we aimed to assess the relationship between phosphate, FGF23, inflammation and iron metabolism in this cohort.

Methods
We measured the plasma concentrations of intact fibroblast growth factor 23 (iFGF23) and selected proinflammatory cytokines at baseline and Week 12 after initiating treatment.

Results
We observed a strong correlation between iFGF23 and cFGF23 (C-terminal fragment plus iFGF23). We identified iFGF23 as a better predictor of changes in serum phosphate induced by NAMR and PB treatment compared with cFGF23. Recursive partitioning revealed at baseline and Week 12, that iFGF23 and cFGF23 together with T50 propensity were the most important predictors of serum phosphate, whereas intact parathyroid hormone (iPTH) played a minor role in this model. Furthermore, we found serum phosphate and iPTH as the best predictors of iFGF23 and cFGF23. Sex, age, body mass index, and markers of inflammation and iron metabolism had only a minor impact in predicting FGF23.

Conclusion
Lowering serum phosphate in ESKF patients may depend highly on iFGF23 which is correlated to cFGF23 levels. Serum phosphate was the most important predictor of plasma FGF23 in this ESKF cohort.
The importance of being the morning case in adult cardiac surgery: a propensity-matched analysis
Authors: Ranucci Marco , Casalino Stefano , Frigiola Alessandro , Diena Marco , Parolari Alessandro , Boveri Sara , Menicanti Lorenzo , De Vincentiis Carlo 
Published: March 2023

AbstractOBJECTIVESThe quality of the outcome after cardiac surgery with cardiopulmonary bypass depends on the patient demographics, co-morbidities, complexity of the surgical procedure and expertise of surgeons and the whole staff. The purpose of the present study is to analyse the timing of surgery (morning versus afternoon) with respect to morbidity and mortality in adult cardiac surgery.METHODSThe primary end point was the incidence of major morbidity defined according to a modified Society of Thoracic Surgeon criterion. We consecutively included all the adult (&gt;18 years) patients receiving a cardiac surgery operation at our Institution.RESULTSFrom 2017 through 2019, a total of 4003 cardiac surgery patients were operated. With a propensity-matching technique a final patient population of 1600 patients was selected, with 800 patients in the first-case surgery group and 800 in the second-case surgery group. Patients in the second-case group had a major morbidity rate of 13% vs 8.8% in the first-case group (P = 0.006), and a higher rate of 30-day mortality (4.1% vs 2.3%, P = 0.033). After correction for EuroSCORE and operating surgeon, the second-case group confirmed a higher rate of major morbidity (odds ratio 1.610, 95% confidence interval 1.16–2.23, P = 0.004).CONCLUSIONOur study suggests that patients operated as second cases are exposed to an increased morbidity and mortality probably due to fatigue, loss of attention and hurriedness in the operating room and decreased human resources in the intensive care unit.
Peri-procedural anticoagulation in patients with end-stage kidney disease undergoing atrial fibrillation ablation: results from the multicentre end-stage kidney disease–atrial fibrillation ablation registry
Authors: Yamamoto Tasuku , Miyazaki Shinsuke , Tanaka Yasuaki , Kono Toshikazu , Nakata Tadanori , Mizukami Akira , Aoyama Daisetsu , Arai Hirofumi , Taomoto Yuta , Horie Tomoki , Hojo Rintaro , Kawamoto Shiho , Yabe Kento , Akiyoshi Kikou , Kato Nobutaka , Ono Yuichi , Suzuki Atsushi , Fukamizu Seiji , Nagata Yasutoshi , Yamauchi Yasuteru , Tada Hiroshi , Hachiya Hitoshi , Inaba Osamu , Takahashi Atsushi , Goya Masahiko , Sasano Tetsuo 
Published: March 2023

AbstractAimsThe optimal anticoagulation regimen in patients with end-stage kidney disease (ESKD) undergoing atrial fibrillation (AF) catheter ablation is unknown. We sought to describe the real-world practice of peri-procedural anticoagulation management in patients with ESKD undergoing AF ablation.Methods and resultsPatients with ESKD on haemodialysis undergoing catheter ablation for AF in 12 referral centres in Japan were included. The international normalized ratio (INR) before and 1 and 3 months after ablation was collected. Peri-procedural major haemorrhagic events as defined by the International Society on Thrombosis and Haemostasis, as well as thromboembolic events, were adjudicated. A total of 347 procedures in 307 patients (67 ±9 years, 40% female) were included. Overall, INR values were grossly subtherapeutic [1.58 (interquartile range: 1.20–2.00) before ablation, 1.54 (1.22–2.02) at 1 month, and 1.22 (1.01–1.71) at 3 months]. Thirty-five patients (10%) suffered major complications, the majority of which was major bleeding (19 patients; 5.4%), including 11 cardiac tamponade (3.2%). There were two peri-procedural deaths (0.6%), both related to bleeding events. A pre-procedural INR value of 2.0 or higher was the only independent predictor of major bleeding [odds ratio, 3.3 (1.2–8.7), P = 0.018]. No cerebral or systemic thromboembolism occurred.ConclusionDespite most patients with ESKD undergoing AF ablation showing undertreatment with warfarin, major bleeding events are common while thromboembolic events are rare.
Bmp-signaling and the finfold size in zebrafish: implications for the fin-to-limb transition
Authors: Cadete Francisco , Francisco Miguel , Freitas Renata 
Published: March 2023

Abstract
In tetrapods, BMP-signaling coordinates limb outgrowth, skeleton patterning, and apoptosis during the formation of their typical autopod structures, the digits. In addition, inhibition of BMP signaling during mouse limb development leads to the persistence and enlargement of an important signaling center, the apical ectodermal ridge (AER), and consequent digit defects. Interestingly, during fish fin development there is a natural elongation of the AER, rapidly converted into an apical finfold (FF), in which osteoblasts differentiate into dermal fin-rays used in aquatic locomotion. Previous reports have led us to suggest that upregulation of Hox13 genes in the distal fin mesenchyme, caused by the origin of novel enhancer modules, may have caused an increment of the BMP signaling potentially leading to the apoptosis of these osteoblasts precursors of the fin-rays. To explore this hypothesis, we characterized the expression of several components of the BMP signaling in zebrafish lines with distinct FF sizes (bmp2b, smad1, smoc1, smoc2, grem1a, msx1b, msx2b, Psamd1/5/9). Our data suggest that the BMP signaling is enhanced in shorter FFs and inhibited in longer FFs, as implied by the differential expression of several components of this network. In addition, we detected an earlier expression of several of these BMP-signaling components associated with the development of short FFs and the opposite tendency during the development of longer FFs. Thus, our results suggest that a heterochronic shift, involving enhanced Hox13 expression and BMP signaling may have caused the reduction of the FF size during the evolutionary transition from fish fins to tetrapod limbs.
Potential Anti-Mpox Virus Activity of Atovaquone, Mefloquine, and Molnupiravir, and Their Potential Use as Treatments
Authors: Akazawa Daisuke , Ohashi Hirofumi , Hishiki Takayuki , Morita Takeshi , Iwanami Shoya , Kim Kwang Su , Jeong Yong Dam , Park Eun-Sil , Kataoka Michiyo , Shionoya Kaho , Mifune Junki , Tsuchimoto Kana , Ojima Shinjiro , Azam Aa Haeruman , Nakajima Shogo , Park Hyeongki , Yoshikawa Tomoki , Shimojima Masayuki , Kiga Kotaro , Iwami Shingo , Maeda Ken , Suzuki Tadaki , Ebihara Hideki , Takahashi Yoshimasa , Watashi Koichi 
Published: March 2023

Abstract

Background
Mpox virus (MPXV) is a zoonotic orthopoxvirus and caused an outbreak in 2022. Although tecovirimat and brincidofovir are approved as anti-smallpox drugs, their effects in mpox patients have not been well documented. In this study, by a drug repurposing approach, we identified potential drug candidates for treating mpox and predicted their clinical impacts by mathematical modeling.

Methods
We screened 132 approved drugs using an MPXV infection cell system. We quantified antiviral activities of potential drug candidates by measuring intracellular viral DNA and analyzed the modes of action by time-of-addition assay and electron microscopic analysis. We further predicted the efficacy of drugs under clinical concentrations by mathematical simulation and examined combination treatment.

Results
Atovaquone, mefloquine, and molnupiravir exhibited anti-MPXV activity, with 50% inhibitory concentrations of 0.51–5.2 μM, which was more potent than cidofovir. Whereas mefloquine was suggested to inhibit viral entry, atovaquone and molnupiravir targeted postentry processes. Atovaquone was suggested to exert its activity through inhibiting dihydroorotate dehydrogenase. Combining atovaquone with tecovirimat enhanced the anti-MPXV effect of tecovirimat. Quantitative mathematical simulations predicted that atovaquone can promote viral clearance in patients by 7 days at clinically relevant drug concentrations.

Conclusions
These data suggest that atovaquone would be a potential candidate for treating mpox.
Scaling up quality in an anesthesia practice
Authors: Dutton Richard P , Swygert Thomas H , Maloney Matthew , Azam Mo , Jones D Kurt , Lounsbury Olivia , Shukla Aesha S , Taheri Paul 
Published: March 2023

AbstractThe resources necessary to improve anesthesia quality and meet reimbursement and regulatory thresholds are scarce, particularly for smaller practices. We examined how small practice integration into a firm with greater resources can facilitate improvements. A mixed-methods analysis was conducted using the data from the US Anesthesia Partners data warehouse, Merit-based Incentive Payment System (MIPS), commercial insurers’ surgery length of stay (LOS) databases, anesthesia-specific patient satisfaction surveys, and interviews with practice leadership before and after integration. All integrated practices improved their quality improvement infrastructure and achieved higher MIPS scores, with increased clinician and leadership satisfaction. Patient satisfaction exceeded national benchmarks in all groups, based on 398 392 returned surveys in 2021. Hospital LOS for common operations was shorter, based on a statewide database. This case study demonstrates that partnership with an organization with greater resources can advance anesthesia quality.
Evaluation of altered environmental conditions as a decontamination approach for SARS-CoV-2 when applied to aircraft related materials
Authors: Richter William , Sunderman Michelle , Fulton Megan , Catyb Joseph , Lewis Douglas 
Published: March 2023

Abstract

Aims
The purpose of this study was to evaluate the effects of altered environmental conditions, specifically elevated temperature at various levels of expected relative humidity (RH), on the inactivation of SARS-CoV-2 when applied to U.S. Air Force aircraft materials.

Methods and results
SARS CoV-2 (USA-WA1/2020) was spiked (∼1 × 105 TCID50) in either synthetic saliva or lung fluid, dried onto porous (e.g. Nylon strap) and nonporous materials (e.g. bare aluminum, silicone, and ABS plastic), placed in a test chamber and exposed to environmental conditions ranging from 40 to 51.7 °C and RH ranging from 0% to 50%. The amount of infectious SARS-CoV-2 was then assessed at various timepoints from 0 to 2 days. Warmer test temperatures, higher RH, and longer exposure duration resulted in higher inactivation rates per material type. Synthetic saliva inoculation vehicle was more readily decontaminated compared to materials inoculated with synthetic lung fluid.

Conclusions
SARS-CoV-2 was readily inactivated below limit of quantitation (LOQ) for all materials inoculated using synthetic saliva vehicle within 6 hours when exposed to environmental conditions of 51.7 °C and RH ≥ 25%. Synthetic lung fluid vehicle did not follow the general trend of an increase in RH resulting in increased efficacy. The lung fluid performed best at the 20%–25% RH range to achieve complete inactivation below LOQ.
Prospects for classical biological control ofSpodoptera frugiperda(Lepidoptera: Noctuidae) in invaded areas using parasitoids from the Americas
Authors: Kenis Marc 
Published: March 2023

AbstractFall armyworm, Spodoptera frugiperda (J.E. Smith) is a polyphagous agricultural pest threatening food security worldwide. This American species recently invaded most of Africa, many Asian countries, and Oceania, where it mainly damages maize. Classical biological control (CBC) through the introduction of natural enemies from its area of origin is considered as a potential management approach. The paper reviews the prospects and constraints of a CBC programme against S. frugiperda using larval parasitoids, which are considered the most suitable natural enemies for introduction against this pest. The most important larval parasitoids in its native range are presented and discussed for their suitability as CBC agents, based the following criteria: their frequency of occurrence and parasitism levels, specificity, climatic suitability and absence of closely related species parasitizing S. frugiperda in the area of introduction. The ichneumonid Eiphosoma laphygmae Costa-Lima (Hymenoptera: Icheumonidae) is considered as a potential candidate for introduction because of its specificity and its importance as a parasitoid of the pest in most of its native range. The most frequent and important parasitoid of S. frugiperda in the Americas, the braconid Chelonus insularis Cresson (Hymenoptera: Braconidae), would most probably contribute to the control of S. frugiperda if released in invaded areas. However, it is oligophagous and would most certainly parasitize nontarget species. Before introducing C. insularis, or any other parasitoid species, the potential nontarget effects will have to be assessed and the risks will have to be weighed against the benefits of improving the natural control of this important pest.
Preperitoneal herniation after transabdominal preperitoneal patch plasty: a case report
Authors: Shirakabe Katsudai , Hirotada Muramatsu , Kanzaki Masaki 
Published: March 2023

AbstractHernial repair is a common procedure performed by general surgeons. The use of laparoscopic hernial repair has recently increased; it was introduced in the 1980s, is minimally invasive and has few complications. However, because of this increase, rare complications that were previously unknown have been reported. A 63-year-old man underwent transabdominal preperitoneal patch plasty (TAPP) for a left inguinal hernia. Tension was felt in the peritoneum during peritoneal closure. On the fifth postoperative day, the patient was admitted for small bowel obstruction (SBO) and underwent reoperation; the closed peritoneum was lacerated, causing SBO because of herniation of the preperitoneal space. After the hernia was released, the peritoneum was closed again, and the surgery was completed. SBO after TAPP surgery is a rare complication. Several reports have shown staplers, barbed sutures and tacks causing SBO; this complication can be prevented with appropriate peritoneal closure techniques and treated with early laparoscopic surgery.
Epstein–Barr virus-positive primary diffuse large B-cell lymphoma of the urinary bladder: a case report
Authors: Sain Baijaeek , Blake Megan , Goyal Kanchan , Kaur Harsimran , Robinson Kristina 
Published: March 2023

AbstractPrimary non-Hodgkin’s lymphoma (NHL) of the urinary bladder is a rare event, with diffuse large B-cell lymphoma (DLBCL) being the most common form of NHL and urinary bladder lymphoma. It is an aggressive tumour with a poor prognosis if not recognised and treated early. The diagnosis is supported by radiological imaging and confirmed by histology, which shows the characteristic morphology of this lesion with further immunohistochemical analysis. Here we present a case of Epstein–Barr virus-positive DLBCL confirmed by an immunohistochemistry panel, along with a brief review of the literature focusing on diagnosis, treatment and outcome of this rare tumour.
A Bayesian approach to modelling spectrometer data chromaticity corrected using beam factors – I. Mathematical formalism
Authors: Sims Peter H , Bowman Judd D , Mahesh Nivedita , Murray Steven G , Barrett John P , Cappallo Rigel , Monsalve Raul A , Rogers Alan E E , Samson Titu , Vydula Akshatha K 
Published: March 2023

ABSTRACT
Accurately accounting for spectral structure in spectrometer data induced by instrumental chromaticity on scales relevant for detection of the 21-cm signal is among the most significant challenges in global 21-cm signal analysis. In the publicly available Experiment to Detect the Global Epoch of Reionization Signature low-band data set, this complicating structure is suppressed using beam-factor-based chromaticity correction (BFCC), which works by dividing the data by a sky-map-weighted model of the spectral structure of the instrument beam. Several analyses of these data have employed models that start with the assumption that this correction is complete. However, while BFCC mitigates the impact of instrumental chromaticity on the data, given realistic assumptions regarding the spectral structure of the foregrounds, the correction is only partial. This complicates the interpretation of fits to the data with intrinsic sky models (models that assume no instrumental contribution to the spectral structure of the data). In this paper, we derive a BFCC data model from an analytical treatment of BFCC and demonstrate using simulated observations that, in contrast to using an intrinsic sky model for the data, the BFCC data model enables unbiased recovery of a simulated global 21-cm signal from beam-factor chromaticity-corrected data in the limit that the data are corrected with an error-free beam-factor model.
A candidate magnetic helium-core white dwarf in the globular cluster NGC 6397
Authors: Pichardo Marcano Manuel , Rivera Sandoval Liliana E , Maccarone Thomas J , Rohrmann Rene D , Heinke Craig O , Belloni Diogo , Althaus Leandro G , Bahramian Arash 
Published: March 2023

ABSTRACTWe report a peculiar variable blue star in the globular cluster (GC) NGC 6397, using Hubble Space Telescope optical imaging. Its position in the colour–magnitude diagrams and its spectrum are consistent with this star being a helium-core white dwarf (He WD) in a binary system. The optical light curve shows a periodicity at 18.5 h. We argue that this periodicity is due to the rotation of the WD and possibly due to magnetic spots on the surface of the WD. This would make this object the first candidate magnetic He WD in any GC, and the first candidate magnetic WD in a detached binary system in any GC and one of the few He WDs with a known rotation period and of magnetic nature. Another possibility is that this system is an He WD in a binary system with another WD or another degenerate object, which would make this object one of the few candidate non-accreting double-degenerate binaries in any GC.