bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engaging Patients and Other Stakeholders in “Designing for Dissemination” of Record Linkage Methods and Tools
Authors: Reno Jenna E. , Ong Toan C. , Voong Chan , Morse Brad , Ytell Kate , Koren Ramona , Kwan Bethany M. 
Published: June 2023

Abstract
Background Novel record linkage (RL) methods have the potential to enhance clinical informatics by integrating patient data from multiple sources—including electronic health records, insurance claims, and digital health devices—to inform patient-centered care. Engaging patients and other stakeholders in the use of RL methods in patient-centered outcomes research (PCOR) is a key step in ensuring RL methods are viewed as acceptable, appropriate, and useful. The University of Colorado Record Linkage (CURL) platform empowers the use of RL in PCOR.
Objectives This study aimed to describe the process of engaging patients and other stakeholders in the design of an RL dissemination package to support the use of RL methods in PCOR.
Methods Customer discovery, value proposition design, and user experience methods were used to iteratively develop an RL dissemination package that includes animated explainer videos for patients and an RL research planning workbook for researchers. Patients and other stakeholders (researchers, data managers, and regulatory officials) were engaged in the RL dissemination package design.
Results Patient partners emphasized the importance of conveying how RL methods may benefit patients and the rules researchers must follow to protect the privacy and security of patient data. Other stakeholders described accuracy, flexibility, efficiency, and data security compared with other available RL solutions. Dissemination package communication products reflect the value propositions identified by key stakeholders. As prioritized by patients, the animated explainer videos emphasize the data privacy and security processes and procedures employed when performing research using RL. The RL workbook addresses researchers' and data managers' needs to iteratively design RL projects and provides accompanying resources to alleviate leadership and regulatory officials' concerns about data regulation compliance.
Conclusion Dissemination products to promote adoption and use of CURL include materials to facilitate patient engagement in RL research and investigator step-by-step decision-making materials about the integration of RL methods in PCOR.
Investment effect of regional trade agreements: an analysis from the perspective of heterogeneous agreement provisions
Authors: Yue Wen , Lin Qingxia , Xu Siyu 
Published: June 2023

AbstractGlobal regional trade agreements (RTAs) are experiencing unprecedented rapid development in terms of number and content of provisions, and China’s RTAs are no exception. With the increase in the depth of RTA provisions, as an important form of economic and trade relations between countries, investment is bound to be affected. Taking the RTAs signed by China as the research object and using the bilateral foreign direct investment (FDI) data provided by the International Monetary Fund CDIS database and the relevant data from the World Bank RTA text depth database, this paper constructs various indexes to measure the depth of RTA provisions and analyzes the effects of changes in the depth of RTA provisions on inward FDI stocks to China. The study finds that the improvement of all four depth indexes, namely, total depth, core depth, depth of WTO+ provisions, and depth of WTO-X provisions, significantly promotes China’s inward FDI stocks. This conclusion still holds after a series of endogenous problem analysis and robustness tests. Further analysis shows that the improvement of the depth of RTA provisions has a relatively greater effect on FDI stocks from developing countries and countries along the “Belt and Road”. At the same time, there exists significant heterogeneity in the effects of different RTA provisions on the FDI stocks. The insights that we obtain in this paper enable us to provide policy recommendations for the construction of China’s global high-standard free trade agreement (FTA) network.
Sociocultural adaptation and job satisfaction as mediators between cultural competence and intention to stay among Vietnamese workers in Taiwan
Authors: Tsang Seng-Su , Nguyen Thi Vinh Tran 
Published: June 2023

AbstractThis study employs a moderated mediation model to investigate the role of sociocultural adaptation and job satisfaction in the relationship between cultural competence and intention to stay. It also considers the moderating function of local language proficiency and length of residence. A survey was conducted among Vietnamese migrant workers employed in Taiwan’s manufacturing industry under the labour export programme. This resulted in a valid dataset of 636 respondents, which was used to test the proposed hypotheses. The findings demonstrated that sociocultural adaptation and job satisfaction fully mediated the relationship between cultural competence and workers’ intention to stay. Moreover, local language proficiency and length of residence were found to substantially strengthen the positive relationship between cultural competence and sociocultural adaptation, but not to moderate the association between cultural competence and job satisfaction. Theoretically, the study contributes to the literature on psychological and behavioural management by examining how migrant workers’ job satisfaction and sociocultural adaptation are related to the association between cultural competence and intention to stay. Practically, the study addresses current concerns regarding the rate of job retention among migrant workers participating in labour export programmes. Its findings provide policymakers and employers with additional evidence to improve and effectively maintain their foreign labour force. It is recommended that both sending and receiving governments implement appropriate and effective management policies to manage the low-skilled foreign labour force.
High-directivity and compact microstrip coupler for RF power applications
Authors: Chan Cheng-Hsuan , Yu Tsung-Chi , Chang Tsun-Hsu 
Published: June 2023

A microstrip coupler was designed, fabricated, and tested to monitor the power of the 500 MHz kW-level amplifier modules currently installed in an 80-kW solid-state transmitter. The proposed coupler consists of multi-section coupled lines and employs a reverse-coupling scheme, achieving a very compact structure. The dual-side configuration with good termination enables the coupler to have high directivity and wide bandwidth without using any lump components for better temperature stability. Under a low-power test, the measured coupling is 37.69 dB with high directivity from 82 to 700 MHz. The coupling is almost independent of the power at 499.65 MHz, while the directivity slightly decreases to 33.85 dB at the input power of 1 kW. The maximal insertion loss of 0.08 dB was observed at 1-kW input power. The proposed directional coupler suits many high-power applications, especially in low-frequency regimes.
Protein–ligand interactions from a quantum fragmentation perspective: The case of the SARS-CoV-2 main protease interacting with α-ketoamide inhibitors
Authors: Genovese Luigi , Dawson William , Nakajima Takahito , Cristiglio Viviana , Vallet Valérie , Masella Michel 
Published: June 2023

We present a hybrid, multi-method, computational scheme for protein/ligand systems well suited to be used on modern and forthcoming massively parallel computing systems. The scheme relies on a multi-scale polarizable molecular modeling, approach to perform molecular dynamics simulations, and on an efficient Density Functional Theory (DFT) linear scaling method to post-process simulation snapshots. We use this scheme to investigate recent α-ketoamide inhibitors targeting the main protease of the SARS-CoV-2 virus. We assessed the reliability and the coherence of the hybrid scheme, in particular, by checking the ability of MM and DFT to reproduce results from high-end ab initio computations regarding such inhibitors. The DFT approach enables an a posteriori fragmentation of the system and an investigation into the strength of interaction among identified fragment pairs. We show the necessity of accounting for a large set of plausible protease/inhibitor conformations to generate reliable interaction data. Finally, we point out ways to further improve α-ketoamide inhibitors to more strongly interact with particular protease domains neighboring the active site.
The application of catch–effort models to estimate the efficacy of aerial shooting operations on sambar deer (Cervus unicolor)
Authors: Ramsey David S. L. , McMaster Damien , Thomas Elaine 
Published: June 2023

Context Aerial shooting from a helicopter targeting introduced sambar deer (Cervus unicolor) is a key activity being undertaken on public land in the North East and East Gippsland regions of Victoria. However, there is currently little published information on the efficacy of aerial shooting for reducing sambar deer populations in Australia. Aims The aims of this study were to analyse the operational data collected during an aerial shooting program in eastern Victoria, to assess the efficacy of aerial shooting at reducing sambar deer density and to inform management decisions on the required intensity of aerial shooting to achieve target densities. Methods Operational data (locations of all shot animals as well as aerial search effort) were analysed from 10 sites using a Bayesian generalised catch–effort model, which allowed for population changes between five periods of intensive control. The model allowed estimates of initial and residual abundance for each site to be made from the catch–effort data, which were used to estimate the efficacy of aerial shooting. Estimates of the detection rate of deer, which were allowed to vary with removal occasion and site, were then used to estimate the amount of aerial search effort required to reduce population densities by various proportional amounts. Key results Aerial shooting resulted in population reductions of 50–70% of sambar deer at four sites where aerial search intensities per unit area were highest. However, results at the remaining sites suggest that sambar deer densities have either remained static or increased over the five periods of aerial control. Recruitment of sambar deer between control periods, which was strongly influenced by study site elevation and season, was largely responsible for eroding reductions achieved by aerial shooting. Conclusions Catch–effort models applied to operational data collected during aerial shooting programs can be used to estimate control efficacy without the need for additional monitoring. Our analysis suggests that sufficiently high search intensities, around 1.4 km of search effort per km2 of habitat in each of five removal occasions, would need to be applied to achieve at least a 50% reduction in sambar deer densities.
A molecular rotor FLIM probe reveals dynamic coupling between mitochondrial inner membrane fluidity and cellular respiration
Authors: Singh Gaurav , George Geen , Raja Sufi O. , Kandaswamy Ponnuvel , Kumar Manoj , Thutupalli Shashi , Laxman Sunil , Gulyani Akash 
Published: June 2023

The inner mitochondrial membrane (IMM), housing components of the electron transport chain (ETC), is the site for respiration. The ETC relies on mobile carriers; therefore, it has long been argued that the fluidity of the densely packed IMM can potentially influence ETC flux and cell physiology. However, it is unclear if cells temporally modulate IMM fluidity upon metabolic or other stimulation. Using a photostable, red-shifted, cell-permeable molecular-rotor, Mitorotor-1, we present a multiplexed approach for quantitatively mapping IMM fluidity in living cells. This reveals IMM fluidity to be linked to cellular-respiration and responsive to stimuli. Multiple approaches combining in vitro experiments and live-cell fluorescence (FLIM) lifetime imaging microscopy (FLIM) show Mitorotor-1 to robustly report IMM ‘microviscosity’/fluidity through changes in molecular free volume. Interestingly, external osmotic stimuli cause controlled swelling/compaction of mitochondria, thereby revealing a graded Mitorotor-1 response to IMM microviscosity. Lateral diffusion measurements of IMM correlate with microviscosity reported via Mitorotor-1 FLIM-lifetime, showing convergence of independent approaches for measuring IMM local-order. Mitorotor-1 FLIM reveals mitochondrial heterogeneity in IMM fluidity; between-and-within cells and across single mitochondrion. Multiplexed FLIM lifetime imaging of Mitorotor-1 and NADH autofluorescence reveals that IMM fluidity positively correlates with respiration, across individual cells. Remarkably, we find that stimulating respiration, through nutrient deprivation or chemically, also leads to increase in IMM fluidity. These data suggest that modulating IMM fluidity supports enhanced respiratory flux. Our study presents a robust method for measuring IMM fluidity and suggests a dynamic regulatory paradigm of modulating IMM local order on changing metabolic demand.
Sensory and motor representations of internalized rhythms in the cerebellum and basal ganglia
Authors: Kameda Masashi , Niikawa Koichiro , Uematsu Akiko , Tanaka Masaki 
Published: June 2023

Both the cerebellum and basal ganglia are involved in rhythm processing, but their specific roles remain unclear. During rhythm perception, these areas may be processing purely sensory information, or they may be involved in motor preparation, as periodic stimuli often induce synchronized movements. Previous studies have shown that neurons in the cerebellar dentate nucleus and the caudate nucleus exhibit periodic activity when the animals prepare to respond to the random omission of regularly repeated visual stimuli. To detect stimulus omission, the animals need to learn the stimulus tempo and predict the timing of the next stimulus. The present study demonstrates that neuronal activity in the cerebellum is modulated by the location of the repeated stimulus and that in the striatum (STR) by the direction of planned movement. However, in both brain regions, neuronal activity during movement and the effect of electrical stimulation immediately before stimulus omission were largely dependent on the direction of movement. These results suggest that, during rhythm processing, the cerebellum is involved in multiple stages from sensory prediction to motor control, while the STR consistently plays a role in motor preparation. Thus, internalized rhythms without movement are maintained as periodic neuronal activity, with the cerebellum and STR preferring sensory and motor representations, respectively.
Adaptive and maladaptive introgression in grapevine domestication
Authors: Xiao Hua , Liu Zhongjie , Wang Nan , Long Qiming , Cao Shuo , Huang Guizhou , Liu Wenwen , Peng Yanling , Riaz Summaira , Walker Andrew M. , Gaut Brandon S. , Zhou Yongfeng 
Published: June 2023

Domesticated grapevines spread to Europe around 3,000 years ago. Previous studies have revealed genomic signals of introgression from wild to cultivated grapes in Europe, but the time, mode, genomic pattern, and biological effects of these introgression events have not been investigated. Here, we studied resequencing data from 345 samples spanning the distributional range of wild (
Vitis vinifera
ssp.
sylvestris
) and cultivated (
V. vinifera
ssp.
vinifera
) grapes. Based on machine learning–based population genetic analyses, we detected evidence for a single domestication of grapevine, followed by continuous gene flow between European wild grapes (EU) and cultivated grapes over the past ~2,000 y, especially from EU to wine grapes. We also inferred that soft-selective sweeps were the dominant signals of artificial selection. Gene pathways associated with the synthesis of aromatic compounds were enriched in regions that were both selected and introgressed, suggesting EU wild grapes were an important resource for improving the flavor of cultivated grapes. Despite the potential benefits of introgression in grape improvement, the introgressed fragments introduced a higher deleterious burden, with most deleterious SNPs and structural variants hidden in a heterozygous state. Cultivated wine grapes have benefited from adaptive introgression with wild grapes, but introgression has also increased the genetic load. In general, our study of beneficial and harmful effects of introgression is critical for genomic breeding of grapevine to take advantage of wild resources.
Cross-talk between phospholipid synthesis and peptidoglycan expansion by a cell wall hydrolase
Authors: Som Nilanjan , Reddy Manjula 
Published: June 2023

The Gram-negative bacterial cell envelope is a complex multilayered structure comprising a bilayered phospholipid (PL) membrane that surrounds the cytoplasm (inner membrane or IM) and an asymmetric outer membrane (OM) with PLs in the inner leaflet and lipopolysaccharides in the outer leaflet. Between these two layers is the periplasmic space, which contains a highly cross-linked mesh-like glycan polymer, peptidoglycan (PG). During cell expansion, coordinated synthesis of each of these components is required to maintain the integrity of the cell envelope; however, it is currently not clear how such coordination is achieved. In this study, we show that a cross-link-specific PG hydrolase couples the expansion of PG sacculus with that of PL synthesis in the Gram-negative model bacterium,
Escherichia coli
. We find that unregulated activity of a PG hydrolytic enzyme, MepS is detrimental for growth of
E. coli
during fatty acid (FA)-limiting conditions. Further genetic and biochemical analyses revealed that cellular availability of FA or PL alters the post-translational stability of MepS by modulating the proteolytic activity of a periplasmic adaptor-protease complex, NlpI-Prc toward MepS. Our results indicate that loss of OM lipid asymmetry caused by alterations in PL abundance leads to the generation of a signal to the NlpI-Prc complex for the stabilization of MepS, which subsequently cleaves the cross-links to facilitate expansion of PG. In summary, our study shows the existence of a molecular cross-talk that enables coordinated expansion of the PG sacculus with that of membrane synthesis for balanced cell-envelope biogenesis.
Activator-induced conformational changes regulate division-associated peptidoglycan amidases
Authors: Cook Jonathan , Baverstock Tyler C. , McAndrew Martin B. L. , Roper David I. , Stansfeld Phillip J. , Crow Allister 
Published: June 2023

AmiA and AmiB are peptidoglycan-hydrolyzing enzymes fromEscherichia coli that are required to break the peptidoglycan layer during bacterial cell division and maintain integrity of the cell envelope. In vivo, the activity of AmiA and AmiB is tightly controlled through their interactions with the membrane-bound FtsEX–EnvC complex. Activation of AmiA and AmiB requires access to a groove in the amidase-activating LytM domain of EnvC which is gated by ATP-driven conformational changes in FtsEX–EnvC complex. Here, we present a high-resolution structure of the isolated AmiA protein, confirming that it is autoinhibited in the same manner as AmiB and AmiC, and a complex of the AmiB enzymatic domain bound to the activating EnvC LytM domain. In isolation, the active site of AmiA is blocked by an autoinhibitory helix that binds directly to the catalytic zinc and fills the volume expected to accommodate peptidoglycan binding. In the complex, binding of the EnvC LytM domain induces a conformational change that displaces the amidase autoinhibitory helix and reorganizes the active site for activity. Our structures, together with complementary mutagenesis work, defines the conformational changes required to activate AmiA and/or AmiB through their interaction with their cognate activator EnvC.
split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80
Authors: Ewen-Campen Ben , Luan Haojiang , Xu Jun , Singh Rohit , Joshi Neha , Thakkar Tanuj , Berger Bonnie , White Benjamin H. , Perrimon Norbert 
Published: June 2023

The split-Gal4 system allows for intersectional genetic labeling of highly specific cell types and tissues in
Drosophila
. However, the existing split-Gal4 system, unlike the standard Gal4 system, cannot be repressed by Gal80, and therefore cannot be controlled temporally. This lack of temporal control precludes split-Gal4 experiments in which a genetic manipulation must be restricted to specific timepoints. Here, we describe a split-Gal4 system based on a self-excising split-intein, which drives transgene expression as strongly as the current split-Gal4 system and Gal4 reagents, yet which is repressible by Gal80. We demonstrate the potent inducibility of “split-intein Gal4” in vivo using both fluorescent reporters and via reversible tumor induction in the gut. Further, we show that our split-intein Gal4 can be extended to the drug-inducible GeneSwitch system, providing an independent method for intersectional labeling with inducible control. We also show that the split-intein Gal4 system can be used to generate highly cell type–specific genetic drivers based on in silico predictions generated by single-cell RNAseq (scRNAseq) datasets, and we describe an algorithm (“Two Against Background” or TAB) to predict cluster-specific gene pairs across multiple tissue-specific scRNA datasets. We provide a plasmid toolkit to efficiently create split-intein Gal4 drivers based on either CRISPR knock-ins to target genes or using enhancer fragments. Altogether, the split-intein Gal4 system allows for the creation of highly specific intersectional genetic drivers that are inducible/repressible.
How can perspectives from Applied Linguistic Historiography
improve our understanding of innovation?
Authors: Smith Richard , Giesler Tim 
Published: June 2023

Abstract
As an emerging interdisciplinary, plurilingual
and intercultural field (McLelland & Smith, 2018), the History of Language
Learning and Teaching – and associated considerations of Applied
Linguistic Historiography (Smith, 2016) – can be expected to provide useful
perspectives on innovation: how, when and why to
attempt it and how to sustain it, but also, from a relatively
philosophical perspective, how to define and assess it. Innovation,
after all, is an activity, or a construct, which is inextricably
bound up with views of the past, whether imagined or
well-researched – a past seen to be in need of replacement or at
least reconstruction. Researching the past can, indeed, give rise to
considerations of whether – and, if so, why and how – innovation
occurs, or is seen to be required at all. In this introduction to a
book devoted to historical perspectives on innovation, we consider
three specific ways in which Applied Linguistic Historiography can
contribute to a revised understanding of innovation in language
teaching and we identify ways in which the chapters in the book shed
new light on its nature, causes, effects and rationale.
La linguistique appliquée
Authors: Whyte Shona 
Published: June 2023

Abstract
At the creation of the Association
Internationale de Linguistique Appliquée (AILA) in
1964, the new field of applied linguistics was marked by
international collaboration on second and foreign language teaching
among linguists in the US, the UK, and France. Over six decades
later, while applied linguistics is a well-established discipline
around the world, la linguistique appliquée is a
much less common term in France. Research on FL teaching is called
la didactique des langues [language didactics]
and involves language teaching theories drawing more on education
theory than applied linguistics. This paper explores the reasons for
this French exception, focusing on its theoretical underpinnings,
institutional foundations and acceptance by teachers and teacher
educators in mid 20th-century France as compared to other founding
countries of AILA.