bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upaya Peningkatan Keterampilan Motorik Halus Anak Usia Dini Di Pekon Negeri Ratu 2 Pesisir Barat Melalui Lukisan Teknik Kolase
Authors: Handayana Sri , Zuhairi Zuhairi , Hakim Nasrul 
Published: January 2025

Abstrak
Pengabdian ini bertujuan untuk meningkatkan keterampilan motorik halus anak anak usia dini di Pekon Negri Ratu 2 Kabupaten Pesisir Barat. Pengabdian ini menggunakan metode Participatory Action Research (PAR) yang dilaksanakan secara partisipatif anak-anak usia dini dalam kegiatan membuat karya lukisan dengan teknik kolase dan memanfaatkan sumberdaya yang ada di lingkungan tempat tinggal mereka. Peserta pengabdian ini adalah anak-anak usia dini yang berasal dari Pekon Negri Ratu 2 berjumlah 20 anak. Anak-anak dilatih untuk menggunting, mewarnai, menggambar, menempel, dan menyusun objek menjadi lukisan yang indah. Hasil pengabdian ini menunjukkan motivasi yang baik dari peserta dan meningkatnya keterampilan motorik halus yang ditunjukkan dengan hasil karya lukisan teknik kolase yang baik dan indah.

Kata Kunci : Motorik Halus, Teknik Kolase.
Pembinaan Keterampilan Berbahasa Inggris Melalui Media Lagu Bagi Anak -Anak Usia Dini di TPA Al-Iman
Authors: Septiyana Linda 
Published: January 2025

ABSTRAK
Pelaksanaan Pengabdian Masyarakat ini bertujuan untuk melatih keterampilan berbahasa Inggris anak-anak di TPA Al-Iman. Media yang digunakan dalam kegiatan pengabdian masyarakat ini adalah media lagu yang berhubungan dengan materi kosakata Bahasa Inggris. Peserta pengabdian masyarakat ini yaitu anak-anak yang ada di TPA AL-Iman Totokaton Punggur. Hasil pengabdian masyarakat ini adalah meningkatnya keterampilan berbahasa Inggris dan pembelajaran yang lebih mudah dipahami dan menyenangkan bagi anak-anak di TPA Al-Iman.

Kata Kunci: Keterampilan Bahasa Inggris, Lagu, Media, Anak Usia Dini,
PELATIHAN IMPLEMENTASI PELAKSANAAN BIMBINGAN KELOMPOK DENGAN PENDEKATAN PROBLEM BASED LEARNING DAN PENYUSUNAN PELAPORANNYA PADA GURU BK DI SMA N 10 KOTA JAMBI
Authors: Yusra Affan , Rasimin Rasimin 
Published: January 2025

Tujuan dari pengabdian masyarakat ini adalah untuk meningkatkan pemahaman dan peningkatan skill untuk guru bimbingan dan konseling sehingga kemampuan dapat ditingkatkan. Adapun sasaran dalam pengabdian masyarakat ini guru bimbingan dan konseling SMAN 10 Kota Jambi pelatihan ini terdiri dai dua sesi dengan jabaran sebagai berikut: 1) Didalam pengabdian ini dibentuk sesuai rancangan untuk meningkatkan pengetahuan guru bimbingan dan konseling kami mengadakan workshop sederhana diruagan bimbinan dan konseling, 2) Metode FGD yang digunakan dalam pembinaan dalam workshop ini merupakan focus untuk meningkatkan skill yang dimiliki oleh guru bimbingan dan konseling yang berada di SMAN 10 Kota Jambi. Dalam hal ini FGD yang dilakukan untuk memberikan keleluasaan bagi guru bimbingan dan konseling untuk bertanya dan mempraktek. Hasil dari pelatihan ini guru bimbigan dan konseling memperoleh pengetahuan yang baru mengenai perluasan ilmu lam bimbingan kelompok hal ini ditunjukan dengan antusias dalam mengikuti pelaksaana. Hasil dari kegiatan PPM ini juga terlihat dalam praktek dan FGDnya guru sangat tertarik dengan metode ini karena dalam metode ini tidak hanya menekankan pada pembentukan dinamika kelompoknamun juga menekankan pada pemikiran kritis anggota kelompok sehingga dalam pelaksanaanya peserta layanannya memperoleh kemampuan berfikir kritis.
A TREATMENT OF A MULTISTEP COLLOCATION METHOD FOR THE DIRECT SOLUTION OF SECOND-ORDER ORDINARY DIFFERENTIAL EQUATIONS
Authors: Atsi Kaze , Tumba Pius , Suleiman Enoch 
Published: January 2025

A research has been conducted at the Department of Mathematics, Faculty of Science, Federal University, Gashua, Yobe State to study the treatment of multistep collocation method for the direct solution of second order ordinary differential equations using a class of modified Backward Differentiation Formular (BDF)-type with one super-future point. The research has proposed the construction of a new method of solving second-order initial valued problem of ordinary differential equation. A step-number, , a number of discrete members are obtained and used in block through multi-step collocation approach. The stability properties of the newly constructed methods are investigated using written computer codes and its convergence are established. The numerical efficiency of the method has been tested on some treated second-order initial valued problems, in order to ascertain its suitability. The solutions of the problems are compared with the corresponding exact solutions and the associated absolute errors are presented. Tables and graph have been adopted in the presentation of results, and they have shown the efficiency of the new method for solving solution of second order ordinary differential equations.
Felnőttoktatással a pozitív társadalmi változásokért és a személyes kiteljesedésért
Authors: Farkas Éva 
Published: January 2025

A Felnőttoktatás és Továbbképzés Hírességeinek Nemzetközi Csarnoka (International
Adult and Continuing Education Hall of Fame, továbbiakban Hírességek Csarnoka) 2024.
november 7-9. között a csodálatos Firenzében szervezte első nagyszabású konferenciáját.
A felnőttoktatás revitalizálása a pozitív társadalmi változásokért és a személyes
kiteljesedésért (Revitalising Adult Continuing Education for Positive Social Changes and
Personal Fulfilment) címet viselő konferencia több szempontból is egyedülálló volt. A
konferenciára a felnőttkori tanulással és oktatással foglalkozó legjelentősebb európai
és észak-amerikai szervezetek, illetve folyóiratok kaptak meghívást. A konferencia abban
is eltért a hagyományos keretektől, hogy csak két plenáris előadás volt, és a résztvevők
tíz munkacsoportban vitatták meg az együttműködés és továbblépés jövőbeli lehetőségeit.
Studi Farmakovigilans Vaksinasi Booster Covid-19 Pada Masyarakat Di Desa Pujon Kalimantan Tengah
Authors: Norlita Devi , Yuwindry Iwan , Aryzki Saftia , Melviani Melviani 
Published: January 2025

Latar belakang: Vaksinasi booster covid-19 diharapkan bisa mencapai terjadinya herd immunity (kekebalan kelompok) membentuk antibodi atau daya tahan tubuh yang kuat sehingga masyarakat bisa bertahan terhadap virus covid-19 dan harapan nya pandemi covid-19 berubah menjadi epidemi covid-19. Reaksi Obat Merugikan (ROM) efek samping yang tidak di inginkan dari suatu obat yang timbul pada pemberian obat dengan dosis yang digunakan untuk profilaksis, diagnosis, dan terapi bersifat aktual atau potensial.Tujuan: Mengetahui Studi Farmakovigilans vaksinasi booster covid-19 pada masyarakat Desa Pujon Kalimantan Tengah.Metode: Penelitian ini adalah Observasional bersifat Kuantitatif. dengan metode penelitian deskriptif, pendekatan yang digunakan dalam penelitian ini adalah cross sectional. Penelitian dilakukan pada bulan Juli di lingkungan Desa Pujon Kalimantan tengah.Hasil: Dari efek samping vaksinasi booster covid-19 yaitu hasil pengukuran Algoritma Naranjo diketahui bahwa dari 98 jumlah responden, dari masyarakat desa pujon paling banyak mendapatkan hasil kategori hasil kategori Naranjo Probable (kemungkinan) merupakan efek samping vaksinasi covid-19 mendapatkan sebanyak 20 responden (20,41%). Diketahui terdapat sebanyak 55 responden mengalami efek samping kemungkinan terjadi. Diantaranya sebanyak 25 demam dan 30 nyeri setelah melakukan vaksinasi covid-19.Simpulan: Dari efek samping vaksinasi booster covid-19 yaitu hasil pengukuran Algoritma Naranjo diketahui bahwa dari 98 jumlah responden, dari masyarakat desa pujon paling banyak mendapatkan hasil kategori hasil kategori Naranjo Possible merupakan kemungkinan efek samping vaksinasi covid-19 mendapatkan sebanyak 55 responden (56,12%). Diketahui terdapat sebanyak 55 responden mengalami kemungkinan efek samping pasti. Diantaranya sebanyak 25 responden mengalami demam dan 30 responden mengalami nyeri setelah melakukan vaksinasi covid-19
Penetapan Kadar Flavonoid Dan Aktivitas Antioksidan Ekstrak Etaanol Daun Bangkal (Nauclea subdita (Korth) Menggunakan Spektrofotometri Uv-Vis
Authors: Ulfah Annisa , Nastiti Kunti , Kurniawati Darini , Hakim Ali Rakhman 
Published: January 2025

Latar belakang: Salah satu Salah satu tanaman khas Kalimantan yang dapat dijadikan sebagai sumber pengobatan dan dapat dijadikan sebagai bahan herbal adalah Tanaman Bangkal. Pada bagian daunnya terdapat berbagai senyawa seperti alkaloid, steroid, tanin dan flavonoid. Senyawa yang mempunyai efek farmakologi kuat dan mempunyai peran sebagai antioksidan adalah senyawa flavonoid.Tujuan: Penelitian ini bertujuan untuk mengetahui kadar flavonoid dan aktivitas antioksidan ekstrak etanol Daun Bangkal (Nauclea subdita (Korth).Metode: Penelitian ini menggunakan metode ekstraksi menggunakan pelarut etanol 70% dan dilakukan perendaman selama 3 × 24 jam, ekstrak kental yang dihasilkan dilakukan uji kualitatif dengan identifikasi senyawa metabolit dan uji kuantitatif dengan penetapan kadar flavonoid serta aktivitas antioksidan.Hasil: Hasil identifikasi senyawa metabolit menggunakan pereaksi warna didapatkan ekstrak etanol Daun Bangkal (Nauclea subdita (Korth) mengandung senyawa alkaloid, steroid, tanin dan flavonoid. Kadar flavonoidnya sebesar 9,524 mgQE/g. Aktivitas antioksidan ekstrak etanol Daun Bangkal (Nauclea subdita (Korth) menunjukkan nilai IC50 sebesar 3,424 ppm.Simpulan: Ekstrak etanol Daun Bangkal (Nauclea subdita (Korth) mengandung senyawa alkaloid, steroid, tanin dan flavonoid. Kadar flavonoid sebesar 9,524 mgQE/g dan aktivitas antioksidannya masuk dalam kategori sangat kuat
Penetapan Kadar Flavonoid Total Dan Aktivitas Antioksidan Daun Karinat (Rubus moluccanus L.)
Authors: Sadlia Fitri , Hakim Ali Rakhman , Saputri Rina , Rohama Rohama 
Published: January 2025

Latar Belakang: Paparan lingkungan dapat memicu pembentukan radikal bebas yang disebut juga reactive oxygen spesies (ROS). Selain disebabkan faktor eksogen, radikal bebas juga dibentuk secara alamiah melalui metabolisme sel fisiologis. Radikal bebas yang tidak ditangani akan memberikan dampak negatif terhadap manusia, diantaranya terjadi perubahan abnormal yang mengenai gen tertentu dalam tubuh yang menyebabkan penyakit kanker. Salah satu senyawa kelompok metabolit sekunder yang memiliki potensi sebagai antioksidan adalah senyawa flavonoid. Ekstrak daun karinat juga terbukti secara kualitatif mengandung senyawa flavonoid berdasarkan hasil Kromatografi Lapis Tipis.Tujuan: Penelitian ini secara umum bertujuan mengetahui kadar total flavonoid dan aktivitas antioksidan pada ekstrak daun karinat (Rubus moluccanus L).Metode: Metode penelitian ini adalah eksperimental laboratorium dengan desain penelitian true-experimental dengan menggunakan rancangan post test only control group design. Kelompok kontrol dalam penelitian ini adalah kuersetin (DPPH) dan variasi konsentrasi ekstrak etanol daun karinat sebagai kelompok perlakuan.Hasil: Hasil penelitian menjelaskan bahwa ekstrak daun karinat memiliki kadar flavonoid total sebesar 143,8 mg/Qe/g dengan nilai rata-rata IC50 sebesar 1,32 ppm yang berarti aktivitas antioksidan daun karinat sangat kuat dan lebih baik daripada kuersetin dengan nilai rata-rata IC50 2,19 ppm. Hasil analisis T-Independent Test diperoleh sebesar 0,000 yang berarti adanya perbedaan aktivitas antioksidan yang signifikan antara daun karinat dan kuersetin.Simpulan: Daun karinat memiliki aktivitas antioksidan yang sangat kuat sehingga daun karinat dapat dimanfaatkan sebagai antioksidan.
Associations between accurate measures of adiposity and fitness, blood proteins, and insulin sensitivity among South Asians and Europeans
Authors: Kho Pik Fang , Stell Laurel , Jimenez Shirin , Zanetti Daniela , Panyard Daniel J. , Watson Kathleen L. , Sarraju Ashish , Chen Ming-Li , Lind Lars , Petrie John R. , Chan Khin N. , Fonda Holly , Kent Kyla , Myers Jonathan N. , Palaniappan Latha , Abbasi Fahim , Assimes Themistocles L. 
Published: January 2025

ObjectiveSouth Asians (SAs) may possess a unique predisposition to insulin resistance (IR). We explored this possibility by investigating the relationship between ‘gold standard’ measures of adiposity, fitness, selected proteomic biomarkers, and insulin sensitivity among a cohort of SAs and Europeans (EURs).MethodsA total of 46 SAs and 41 EURs completed ‘conventional’ (lifestyle questionnaires, standard physical exam) as well as ‘gold standard’ (dual energy X-ray absorptiometry scan, cardiopulmonary exercise test, and insulin suppression test) assessments of adiposity, fitness, and insulin sensitivity. In a subset of 28 SAs and 36 EURs, we also measured the blood-levels of eleven IR-related proteins. We conducted Spearman correlation to identify correlates of steady-state plasma glucose (SSPG) derived from the insulin suppression test, followed by multivariable linear regression analyses of SSPG, adjusting for age, sex and ancestral group.ResultsSixteen of 30 measures significantly associated with SSPG, including one conventional and eight gold standard measures of adiposity, one conventional and one gold standard measure of fitness, and five proteins. Multivariable regressions revealed that gold standard measures and plasma proteins attenuated ancestral group differences in IR, suggesting their potential utility in assessing IR, especially among SAs.ConclusionAncestral group differences in IR may be explained by accurate measures of adiposity and fitness, with specific proteins possibly serving as useful surrogates for these measures, particularly for SAs.
Longitudinal analysis of soil erosion dynamics using the RUSLE model in Ethiopia’s Lake Ziway watershed: implications for agricultural sustainability and food security
Authors: Teku Degfie , Workie Melak Desta 
Published: January 2025

IntroductionSoil erosion is a persistent environmental challenge in Ethiopia’s Lake Ziway Watershed, posing severe risks to agricultural sustainability, food security, and ecosystem stability. Despite its significance, limited longitudinal studies have systematically analyzed soil erosion dynamics in this region or identified critical intervention areas.MethodsThis study employs the Revised Universal Soil Loss Equation (RUSLE) model integrated with remote sensing and GIS techniques to perform a 30-year longitudinal analysis (1993–2023).ResultsThe analysis highlights spatial and temporal trends in both potential and modeled actual soil erosion rates, revealing substantial but fluctuating losses over the study period. In 1993, potential soil erosion ranged from 0 to 2,082 t/ha/yr, totaling approximately 3.76 million tonnes annually. By 2023, this had shifted to a range of 0–2,069 t/ha/yr, with an annual loss of 3.48 million tonnes. Modeled actual soil erosion followed a similar trajectory, decreasing from 2.58 million tonnes in 1993 to 2.30 million tonnes in 2023. Mean soil loss rates for potential erosion increased from 42.91 t/ha/yr in 1993 to 43.73 t/ha/yr in 2023, while modeled actual erosion rates rose from 38.79 t/ha/yr to 41.01 t/ha/yr.DiscussionThese figures consistently exceed Ethiopia’s acceptable soil loss threshold of 10–15 t/ha/y, highlighting the need for targeted interventions, particularly in erosion-prone areas. This study bridges critical knowledge gaps, providing actionable insights for policy development aimed at sustainable land management and agricultural resilience.
Mutation in the COL2A1 gene is associated with acetabular dysplasia
Authors: Xin Miaomiao , Guan Xin , Yang Jiangfei , Li Yi , Man Zhentao , Sun Hongsheng , Fu Min 
Published: January 2025

BackgroundDevelopmental dysplasia of the hip (DDH) is one of the most common developmental disorders worldwide, caused by a combination of genetic and environmental factors.MethodsTo investigate the genetic etiology of DDH in a proband (a 27-year-old male), we reviewed the patient’s clinical data and collected peripheral blood samples from the proband and his parents. Genomic DNA was extracted, and polymerase chain reaction (PCR) amplification was performed. Clinical whole-exome sequencing (WES) using next-generation sequencing (NGS) was conducted to identify potential mutation sites, which were then validated through Sanger sequencing. Bioinformatics analysis was performed to assess the pathogenicity of the identified variant, and 3D protein modeling was conducted to predict its impact on protein structure.ResultsThe proband presented with pain in bilateral hips, and based on clinical symptoms, laboratory findings and imaging studies, the final diagnosis was considered to be acetabular dysplasia with overlapping secondary synovial chondromatosis. Family history revealed similar symptoms in the proband’s father, while the grandparents and other family members were unaffected. The patient underwent bilateral total hip arthroplasty and synovectomy. NGS and Sanger sequencing identified a heterozygous missense mutation in the COL2A1 gene (ex13, c.823C &gt; T; p.Arg275Cys) in both the proband and his father, while this mutation was absent in the mother. Bioinformatic analysis indicated that the c.823C &gt; T (p.Arg275Cys) variant is pathogenic, and structural modeling demonstrated that the substitution of arginine with cysteine at residue 275 altered the protein structure.ConclusionOur findings highlight the diagnostic utility of NGS in identifying precise genetic causes of DDH. The identification of the COL2A1 gene mutation in this present case represents a novel clinical phenotype, expanding the spectrum of disorders associated with COL2A1 mutations.
Effect of trace elements and nutrients on 21 autoimmune diseases: a Mendelian randomization study
Authors: Jia Ming-Jie , Yin Hua-Fang , Liang Ying-Chao , Jiang Feng , Li Hui-Lin 
Published: January 2025

BackgroundNumerous clinical studies have observed a close relationship between serum trace elements, nutrients, and autoimmune diseases. However, whether there is a genetic causal effect between serum trace elements, nutrients, and autoimmune diseases remains unclear.ObjectiveThis study aims to investigate the causal effects of serum trace elements and nutrients on 21 autoimmune diseases using Mendelian randomization (MR).MethodsSingle nucleotide polymorphisms for the exposure factors (serum trace elements and vitamins) were obtained from the published UK Biobank database and genome-wide association study (GWAS) public databases. Outcome GWAS data were derived from the FinnGen database. MR was employed to explore the causal relationships between 9 trace elements and 6 vitamins and autoimmune diseases. Causal inference was performed using inverse variance weighted methods, MR Egger, and weighted median methods. Subsequently, heterogeneity tests, horizontal pleiotropy tests, MR-PRESSO tests, and leave-one-out analyses were conducted for sensitivity analysis to evaluate the robustness of the study results. Finally, trace elements and vitamins that were statistically significant in the IVW method and had consistent effect sizes and odds ratios across five methods were selected as exposure factors with a causal relationship to diabetes and its complications. Additionally, multivariable Mendelian randomization was employed to assess the combined effects of multiple exposure factors on autoimmune diseases.ResultsMR analysis indicated that elevated levels of the trace element copper were associated with an increased risk of systemic lupus erythematosus (SLE) and a decreased risk of ulcerative colitis. Carotene was found to have a negative causal relationship with adult-onset Still’s disease (AOSD). Elevated levels of copper and selenium were associated with an increased risk of autoimmune hyperthyroidism. Calcium levels showed a negative causal relationship with the risk of polyarteritis nodosa. MVMR results demonstrated that selenium could independently affect the risk of autoimmune hyperthyroidism, separate from copper.ConclusionThe findings from both univariable and multivariable Mendelian randomization studies support a causal relationship between trace elements, nutrients, and autoimmune diseases. These results have significant clinical implications for developing targeted prevention and treatment strategies for autoimmune diseases.
The generation and evaluation of TKO/hCD55/hTM/hEPCR gene-modified pigs for clinical organ xenotransplantation
Authors: Huai Guoli , Wang Yong , Du Jiaxiang , Cheng Zhenhui , Xie Yuxuan , Zhou Jia , Tang Hongmei , Jiang Yanyan , Xing Xiangyang , Deng Shaoping , Pan Dengke 
Published: January 2025

IntroductionGenetically edited pigs, modified using CRISPR-Cas9 technology, hold promise as potential sources for xenotransplantation. However, the optimal combination of genetic modifications and their expression levels for initial clinical trials remains unclear. This study investigates the generation of TKO/hCD55/hTM/hEPCR (6GE) pigs and evaluates their compatibility with human immune and coagulation systems.MethodsThe 6GE pigs were generated through iterative genome editing and F1 generation breeding. Genotyping, flow cytometry, and immunohistochemistry confirmed the knockout of GGTA1, CMAH, and B4GALNT2. Expression levels of human genes (hCD55, hTM, hEPCR) were quantified. In vitro assays using aortic endothelial cells (pAECs) from 6GE pigs assessed human serum IgM and IgG binding, complement cytotoxicity, and thrombin-antithrombin (TAT) complex levels. Blood from gene-edited pigs was used for pathophysiological analysis.ResultsComplete knockout of GGTA1, CMAH, and B4GALNT2 was confirmed in 6GE pigs. The expression of hCD55 and hTM was approximately seven and thirteen times higher than in humans, respectively, while hEPCR levels were comparable to those in humans. In vitro, 6GE pAECs showed significantly reduced binding of human IgM and IgG compared to wild-type pAECs (IgG p&lt;0.01, IgM p&lt;0.0001). Similar to TKO/hCD55 pAECs, 6GE pAECs exhibited a substantial reduction in complement-mediated cytotoxicity (p&lt;0.001) compared to TKO pAECs. Co-expression of hTM and hEPCR in 6GE pigs led to a significant decrease in thrombin-antithrombin (TAT) complex levels in co-culture with human whole blood, compared to WT (p&lt;0.0001), TKO (p&lt;0.01), and TKO/hCD55/hTM pigs (p&lt;0.05). Pathophysiological analysis demonstrated excellent compatibility of 6GE pig kidneys and livers with human immune and coagulation systems. However, 6GE pigs showed increased susceptibility to infection compared to other gene-edited pigs, while TKO/hCD55 pigs were considered safe when they were all bred in a general environment.DiscussionHighly expressing hCD55, along with the co-expression of hEPCR and hTM genes, is expected to effectively reduce human complement cytotoxicity and enhance anticoagulant efficacy in genetically modified pigs. The 6GE pigs exhibited robust compatibility with human physiological and immune systems, fulfilling the criteria for clinical trials. Furthermore, it is imperative to rear donor pigs in pathogen-free (DPF) facilities to mitigate infection risks and prevent the transmission of porcine pathogens to humans.