bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical evaluation of a decision support system for glucose infusion in hypoglycaemic clamp experiments
Authors: Pavan Jacopo , Herzig David , Tripyla Afrodity , Dalla Man Chiara , Bally Lia , Del Favero Simone 
Published: September 2023

AbstractAimTo provide a preliminary evaluation of the accuracy and safety of Gluclas decision support system suggestions in a hypoglycaemic clamp study.MethodsThis analysis was performed using data from 32 participants (four groups with different glucose‐insulin regulation: post Roux‐en‐Y gastric bypass with and without postprandial hypoglycaemia syndrome, postsleeve gastrectomy and non‐operated controls) undergoing Gluclas‐assisted hypoglycaemic clamps (target: 2.5 mmol/L for 20 minutes at 150 minutes after oral glucose ingestion). Gluclas provided glucose infusion rate suggestions upon manual entry of blood glucose values (every 5 minutes), which were either followed or overruled by investigators after critical review. Accuracy and safety were evaluated by mean absolute error (MAE), mean absolute percentage error (MAPE), average glucose level, coefficient of variation (CV) and minimal glucose level during the 20‐minute hypoglycaemic period.ResultsInvestigators accepted 84% of suggestions, with a mean deviation of 30.33 mg/min. During the hypoglycaemic period, the MAE was 0.16 (0.12‐0.24) (median [interquartile range]) mmol/L and the MAPE was 6.12% (4.80%‐9.29%). CV was 4.90% (3.58%‐7.27%), with 5% considered the threshold for sufficient quality. The minimal glucose level was 2.40 (2.30‐2.50) mmol/L.ConclusionsGluclas achieved sufficiently high accuracy with minimal safety risks in a population with differences in glucose‐insulin dynamics, underscoring its applicability to various patient groups.
Domestication in dry‐cured meat Penicillium fungi: Convergent specific phenotypes and horizontal gene transfers without strong genetic subdivision
Authors: Lo Ying‐Chu , Bruxaux Jade , Rodríguez de la Vega Ricardo C. , O'Donnell Samuel , Snirc Alodie , Coton Monika , Le Piver Mélanie , Le Prieur Stéphanie , Roueyre Daniel , Dupont Joëlle , Houbraken Jos , Debuchy Robert , Ropars Jeanne , Giraud Tatiana , Branca Antoine 
Published: September 2023

AbstractSome fungi have been domesticated for food production, with genetic differentiation between populations from food and wild environments, and food populations often acquiring beneficial traits through horizontal gene transfers (HGTs). Studying their adaptation to human‐made substrates is of fundamental and applied importance for understanding adaptation processes and for further strain improvement. We studied here the population structures and phenotypes of two distantly related Penicillium species used for dry‐cured meat production, P. nalgiovense, the most common species in the dry‐cured meat food industry, and P. salamii, used locally by farms. Both species displayed low genetic diversity, lacking differentiation between strains isolated from dry‐cured meat and those from other environments. Nevertheless, the strains collected from dry‐cured meat within each species displayed slower proteolysis and lipolysis than their wild conspecifics, and those of P. nalgiovense were whiter. Phenotypically, the non‐dry‐cured meat strains were more similar to their sister species than to their conspecific dry‐cured meat strains, indicating an evolution of specific phenotypes in dry‐cured meat strains. A comparison of available Penicillium genomes from various environments revealed HGTs, particularly between P. nalgiovense and P. salamii (representing almost 1.5 Mb of cumulative length). HGTs additionally involved P. biforme, also found in dry‐cured meat products. We further detected positive selection based on amino acid changes. Our findings suggest that selection by humans has shaped the P. salamii and P. nalgiovense populations used for dry‐cured meat production, which constitutes domestication. Several genetic and phenotypic changes were similar in P. salamii, P. nalgiovense and P. biforme, indicating convergent adaptation to the same human‐made environment. Our findings have implications for fundamental knowledge on adaptation and for the food industry: the discovery of different phenotypes and of two mating types paves the way for strain improvement by conventional breeding, to elucidate the genomic bases of beneficial phenotypes and to generate diversity.
TSC2 inactivation, low mutation burden and high macrophage infiltration characterise hepatic angiomyolipomas
Authors: Giannikou Krinio , Klonowska Katarzyna , Tsuji Junko , Wu Shulin , Zhu Zachary , Probst Clemens K , Kao Katrina Z , Wu Chin‐Lee , Rodig Scott , Marino‐Enriquez Adrian , Zen Yoh , Schaefer Inga‐Marie , Kwiatkowski David J 
Published: September 2023

AimsAlthough TSC1 or TSC2 inactivating mutations that lead to mTORC1 hyperactivation have been reported in hepatic angiomyolipomas (hAML), the role of other somatic genetic events that may contribute to hAML development is unknown. There are also limited data regarding the tumour microenvironment (TME) of hAML. The aim of the present study was to identify other somatic events in genomic level and changes in TME that contribute to tumorigenesis in hAML.Methods and resultsIn this study, we performed exome sequencing in nine sporadic hAML tumours and deep‐coverage targeted sequencing for TSC2 in three additional hAML. Immunohistochemistry and multiplex immunofluorescence were carried out for 15 proteins to characterise the tumour microenvironment and assess immune cell infiltration. Inactivating somatic variants in TSC2 were identified in 10 of 12 (83%) cases, with a median allele frequency of 13.6%. Five to 18 somatic variants (median number: nine, median allele frequency 21%) not in TSC1 or TSC2 were also identified, mostly of uncertain clinical significance. Copy number changes were rare, but detection was impaired by low tumour purity. Immunohistochemistry demonstrated numerous CD68+ macrophages of distinct appearance from Küpffer cells. Multiplex immunofluorescence revealed low numbers of exhausted PD‐1+/PD‐L1+, FOXP3+ and CD8+ T cells.ConclusionhAML tumours have consistent inactivating mutations in TSC2 and have a low somatic mutation rate, similar to other TSC‐associated tumours. Careful histological review, standard IHC and multiplex immunofluorescence demonstrated marked infiltration by non‐neoplastic inflammatory cells, mostly macrophages.
ABCC2 brush‐border expression predicts outcome in papillary renal cell carcinoma: a multi‐institutional study of 254 cases
Authors: Castillo Vincent Francis , Masoomian Mehdi , Trpkov Kiril , Downes Michelle , Brimo Fadi , van der Kwast Theodorus , Yousef George M , Zakhary Abraam , Rotondo Fabio , Saad Gina , Nguyen Vy‐nhan , Kidanewold Wondwossen , Streutker Catherine , Rowsell Corwyn , Hamdani Malek , Saleeb Rola M 
Published: September 2023

AimsPapillary renal cell carcinoma (PRCC) histologic subtyping is no longer recommended in the 2022 WHO classification. Currently, WHO/ISUP nucleolar grade is the only accepted prognostic histologic parameter for PRCC. ABCC2, a renal drug transporter, has been shown to significantly predict outcomes in PRCC. In this study we evaluated the prognostic significance of ABCC2 IHC staining patterns in a large, multi‐institutional PRCC cohort and assessed the association of these patterns with ABCC2 mRNA expression.Methods and resultsWe assessed 254 PRCCs for ABCC2 IHC reactivity patterns that were stratified into negative, cytoplasmic, brush‐border <50%, and brush‐border ≥50%. RNA in situ hybridization (ISH) was used to determine the transcript level of each group. Survival analysis was performed with SPSS and GraphPad software. RNA‐ISH showed that the ABCC2 group with any brush‐border staining was associated with a significant increase in the transcript level, when compared to the negative/cytoplasmic group (P = 0.034). Both ABCC2 groups with brush‐border <50% (P = 0.024) and brush‐border ≥50% (P < 0.001) were also associated with worse disease‐free survival (DFS) in univariate analysis. Multivariate analysis showed that only ABCC2 IHC brush‐border (<50% and ≥50%) reactivity groups (P = 0.037 and P = 0.003, respectively), and high‐stage disease (P < 0.001) had a DFS of prognostic significance. In addition, ABCC2 brush‐border showed significantly worse DFS in pT1a (P = 0.014), pT1 (P = 0.013), ≤4 cm tumour (P = 0.041) and high stage (P = 0.014) groups, while a similar analysis with high WHO/ISUP grade in these groups was not significant.ConclusionABCC2 IHC brush‐border expression in PRCC correlates with significantly higher gene expression and also independently predicts survival outcomes.
A long‐term longitudinal study of gingival recession in dentists: A 15‐year follow‐up
Authors: Matas Francesc , Mendieta Carlos 
Published: September 2023

AbstractObjectivesTo assess the prevalence, distribution pattern, and development/progression of gingival recession in a population of dentists, followed up over an additional 15 years, after an initial and previously published follow‐up of 10 years.MethodsThis 25‐year longitudinal gingival recession investigation started in 1994 at the Dental School with 60 final‐year dental students; among them, 40 were re‐examined in 2004, and 27 of those 40 were further examined in 2019 by the same examiner. A questionnaire session provided information on toothbrushing habits at each appointment.ResultsIn this study, 27 individuals aged 31–44 years (724 teeth) were followed up. The prevalence of gingival recession was 100% after 15 years. A total of 204 and 317 recessions found in 2004 and 2019, respectively, were valid for the longitudinal evaluation. The highest recession incidence was observed in the molars during both examinations (p < 0.0001). The mean recession heights were 1.66 ± 0.87 mm and 1.85 ± 1.08 mm, respectively (p = 0.024). The difference between the means was 0.19 mm after 15 years. The mean probing depth and bleeding on probing at the point of the maximum recession decreased (p < 0.0001). A multiple regression analysis on the tooth level was performed and indicated that the keratinized gingival width was negatively associated with the severity of the buccal recession height.ConclusionsFifteen years after the initial follow‐up, the mean number of gingival recessions per dentist and the mean recession height increased, while toothbrushing habits remained nearly unchanged.
Content marketing research: A review and research agenda
Authors: Bubphapant Jitpisut , Brandão Amélia 
Published: September 2023

AbstractGiven the importance of content that integrates into consumers' daily lives, marketing academics are becoming more interested in content marketing. Although academics have studied digital marketing research for decades, their understanding of content marketing remains limited. The purpose of this study is to perform a comprehensive review of the existing literature on content marketing and an equally comprehensive research analysis in the field. Accordingly, the study synthesizes 112 items of content marketing literature, using bibliometric analysis and the TCCM framework, to examine the evolution of content marketing research over the last 7 years (2015–2021). Furthermore, the study also develops an integrated conceptual framework, showing antecedents, mediators, moderators, and consequences of content marketing. This systematic review demonstrates that content marketing research continues to develop because of emerging technologies and online platforms, which provide opportunities for enhancing content strategies and practices. Finally, the paper suggests certain areas that have been neglected but which would lead to further investigations that advance this area of study. This comprehensive literature study adds to the body of knowledge on content marketing.
Association between statins exposure and risk of skin cancer: an updated meta‐analysis
Authors: Wang Dongying , Dai Senjie , Lou Dandi , Wang Tianyue , Wang Shihui , Zheng Zhen 
Published: September 2023

AbstractThis study aimed to investigate the relationship between statin (lipophilic statin and hydrophilic statin) exposure and the risk of skin cancer. The incidence of skin cancer under statin exposure was used as the primary outcome, and the relevant studies were screened from Web of Science, PubMed, Cochrane Library, and EBSCO electronic database until September 2022. Ten observational studies and two randomized controlled trials (RCTs) were included. The statistical results indicated that in lipophilic statins, the exposed group had a higher risk of skin cancer than the non‐exposed group (OR: 1.09, P = 0.003). However, compared with the non‐exposed group, there was no significant difference between hydrophilic statins exposure and the incidence of skin cancer (OR: 1.02, P = 0.341). Further subgroup analysis of the subtypes of statins revealed that compared with the non‐exposed group, exposure to lovastatin (OR: 1.18, P = 0.048) or simvastatin (OR: 1.11, P < 0.001) was a risk factor for skin cancer. Besides, subgroup analysis based on the subtypes of skin cancer demonstrated that the risks of melanoma (OR: 1.13, P = 0.009), basal cell carcinoma (BCC) (OR: 1.05, P = 0.036), and squamous cell carcinoma (SCC) (OR: 1.13, P = 0.026) under lipophilic statin exposure were significantly higher than those in the non‐exposed group. On the contrary, compared with the non‐exposed group, the risk of BCC was significantly reduced under the exposure of hydrophilic statins (OR: 0.93, P = 0.031). This study showed that the relationship between statin exposure and skin cancer risk was affected by the subtypes of statins and skin cancer subtypes.
Importance of rheological properties in enrobing efficiency of dark chocolate: application in wafer products
Authors: Ertural Gökşen Işık , Gunes Recep , Toker Omer Said , Palabiyik Ibrahim , Konar Nevzat , Sagdic Osman 
Published: September 2023

SummaryIn this study, the rheological properties of dark chocolate used for enrobing in wafer production were modified with the use of lecithin and polyglycerol polyricinoleate (PGPR), and its effects on the final product were investigated. Lecithin was added to the dark chocolate from 0.1 to 0.8 g per 100 g in increments of 0.1 g, then, 0.10, 0.15, and 0.20 g per 100 g PGPR were combined separately with 0.40 g per 100 g lecithin containing sample. There were significant differences in the enrobing ratios, colour, texture, and sensory parameters of the wafer samples (P < 0.05) enrobed with dark chocolate, in which lecithin and PGPR were used together, due to the changes in the flow behaviour of the chocolate. The wafers enrobed with emulsifier‐free dark chocolate had the highest enrobing ratio (72.3%), while it was 45% in the sample enrobed with dark chocolate containing 0.40 g per 100 g lecithin + 0.20 g per 100 g PGPR. In parallel with the decrease in the enrobing ratio, changes occurred in the textural hardness and fracturability values of the samples. On the other hand, as the enrobing ratio decreased, the structure of the wafer sheets became more prominent during consumption, resulting in an increase in the sensory evaluation scores.
Tef and fonio‐morphology, determination of amino acids, phenolic compounds and antioxidant capacity of two gluten‐free grains
Authors: Ananth Devanesan Arul , Mahalakshmi Vijayarahavan , Yermiyahu Uri , Van Oss Pinhasi Ruth , Klipcan Liron , Tietel Zipora 
Published: September 2023

SummaryGlobal demand for gluten‐free products is rising, however, currently most lack protein and are nutritionally insufficient. Tef and fonio, two nutritious gluten‐free cereals might provide a good alternative. To evaluate their composition, a detailed profiling of their free amino acid (AA) and phenolic composition was performed, using UPLC‐QqQ‐MS/MS, in addition to TPC and antioxidant activity. Grain morphology was also investigated. Our results reveal the presence of 18 AAs, including all essential, in both tef and fonio. l‐asparagine and l‐tryptophan were the most abundant, whereas cysteine and glutamine were absent. Levels of lysine were sufficient (4.39 and 6.72 mg g−1 DW for tef and fonio, respectively), but levels of methionine in fonio were low (0.45 mg g−1 DW). TPC was 0.87 and 0.38 mg GAE g−1 DW in tef and fonio, respectively. Eleven phenolic compounds were identified and quantified in both cereals, ranging from 2246 μg g−1 DW to trace levels, with higher levels observed in tef. ABTS Antioxidant activity was 0.25 and 0.15 mg TE g−1 DW for tef and fonio, respectively, typical for cereals and millets. Overall, our work implies that tef and fonio can make a healthful contribution to human nutrition, and specifically be incorporated into gluten‐free diet to increase protein and polyphenol consumption.
The resting process: a comprehensive review of the effects on dough properties and resulting noodles quality, including improvement strategies
Authors: Obadi Mohammed , Li Yuntong , Qi Yajing , Xu Bin 
Published: September 2023

SummaryThe production processes of different types of noodles are slightly different, but the main production process includes kneading, resting, rolling, sheeting and cutting. Among them, resting is an important step in noodle‐making that has a major impact on the final quality of the noodles. After the kneading is over, the dough needs to rest for a period of time before it can be pressed into sheets. The effectiveness of the dough resting process led to a more uniform and compact gluten network distribution and improved the texture and cooking properties of the noodles compared to those without resting. From the literature reviewed, reasonable dough resting times, temperatures and humidity can improve the quality of the finished product. This review aims to summarise the concept of the dough resting process and factors affecting dough resting, discuss the effect of the resting process on dough properties and noodle quality, and suggest specific improvement strategies.
Enrichment of antioxidant activity of ice cream samples with addition of rowanberry (Sorbus aucuparia L.) pulp and production of functional probiotic ice cream with using L. rhamnosus
Authors: Goktas Hamza 
Published: September 2023

SummaryRowanberry fruit is rich in functional compounds, therefore, this work goaled to produce functional probiotic ice cream with addition of rowanberry pulp (2.5%, 5%, 10% and 20%) and Lacticaseibacillus rhamnosus. Using rowanberry pulp and probiotic decreased pH and brightness values of the ice creams and changed between 6.65–5.37 and 96.19–73.46, respectively (P ≤ 0.05). Consistency values (49.54–13.94 (Pa.s)) of the ice creams decreased with addition of rowanberry pulp and probiotic while hardness values (236.80–494.50 (g)) increased. Antioxidant capacity of ice creams increased with addition of rowanberry pulp (P ≤ 0.05) and the highest value were determined for D10 sample as 53.08 (%). After 120 days of storage, probiotic viability was higher than 8.24 log CFU g−1 and survival rate of L. rhamnosus was preserved under in vitro digestion with addition of rowanberry pulp. Finally, sensorial scores showed that 5% rowanberry pulp can be used in the manufacturing of functional probiotic ice cream.
Review in edible materials for sustainable cultured meat: scaffolds and microcarriers production
Authors: Moslemy Nima , Sharifi Emad , Asadi‐Eydivand Mitra , Abolfathi Nabiollah 
Published: September 2023

SummaryCultured meat, also known as laboratory‐grown or cell‐based meat, is a promising concept in the food industry that involves culturing animal cells in vitro to produce meat products, bypassing the need for traditional animal rearing and slaughter. This technology offers solutions to key challenges associated with conventional meat production, including environmental sustainability, animal welfare and food security. However, the cultured meat industry must overcome significant hurdles to achieve commercial viability. Scaling up production to meet the increasing demand for meat is a considerable challenge, requiring a scalable edible scaffold, eliminating the need for separate scaffold removal, and ensuring product safety. Various edible materials, including polysaccharides and proteins, along with fabrication techniques like electrospinning and 3D printing, are explored in this review article.
Impact of delirium intervention on cognitive load among nurses in the intensive care unit: A multi‐centre cluster randomized controlled trial
Authors: Zhang Shan , Ji Meihua , Cui Wei , Wei Jun , Ding Shu , Wu Ying 
Published: September 2023

AbstractBackgroundHigh cognitive load in nurses is a common problem in the intensive care unit (ICU). However, it remains unclear what different types of cognitive load the ICU nurses have experienced during the implementation of delirium interventions.AimTo describe the characteristics and explore the effect of implementing a delirium intervention on the cognitive load of nurses working in the ICU.MethodsA cluster‐randomized controlled clinical trial was conducted. Six ICUs were randomized in a 1:1 ratio, and eligible nurses from these units provided either a delirium bundle intervention in addition to usual care (27 nurses) or usual care alone. An instrument was used to measure different types of cognitive load (MDT‐CL), assessing intrinsic, extraneous and germane cognitive load. The repeated measures analysis of variance was used to detect between‐group differences.ResultsAmong these nurses, significant between‐group differences were identified in terms of their overall (P < 0.001), intrinsic (P < 0.001) and extraneous (P < 0.001) cognitive load. There was no significant change observed in the germane cognitive load (P = 0.489) in the delirium intervention group.ConclusionIt is important to understand how the implementation of a delirium intervention affects different types of cognitive load in nurses, in order that tailored strategies can be applied to reduce cognitive load in ICU nurses.
The association of food insecurity on body mass index change in a pediatric weight management intervention
Authors: Persaud Alicia , Evans E. Whitney , Perkins Meghan , Simione Meg , Cheng Erika R. , Luo Mandy , Burgun Rachel , Taveras Elsie M. , Fiechtner Lauren 
Published: September 2023

SummaryBackgroundChildhood obesity is a critical public health concern. One potential determinant to obesity that is less understood is food insecurity.ObjectiveTo examine the association of food security status on body mass index (BMI) change in a Pediatric Weight Management Intervention (PWMI) consistent with national treatment recommendations.MethodsThis analysis included 201 participants from the Healthy Weight Clinic (HWC). Using linear mixed models, we compared BMI and %BMIp95 change per year between the food insecure group and food secure group, adjusting for baseline BMI, age and sex, and SNAP enrolment.ResultsIn fully adjusted models, children in households with food insecurity had a 0.50 (0.26–0.74) kg/m2 BMI increase per year and a 2.10 (1.02–3.19) %BMIp95 increase per year compared to households that were food secure.ConclusionsWhen comparing the BMI effect of the HWC between the food insecure group and food secure group, those experiencing food insecurity in the HWC had an increase in BMI compared to those with food security. These findings suggest that food insecurity may reduce the effectiveness of PWMIs consistent with national recommendations; however, more studies should be conducted to better understand this relationship.