bluuebunny/crossref_metadata_2025_split huggingface dataset:
NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals
Authors: Yang Jingwen , Wu Xi , Aucapiña Cristina belen , Zhang Deyu , Huang Jiazhi , Hao Ziyuan , Zhang Yu , Ren Yujun , Miao Ying 
Published: February 2023

AbstractThe color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant, they are also important in their ornamental appeal in Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis of loss- and gain- of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways. Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS, NtLAR, and NtDFR that were cloned by genome walking assay, and activated NtFLS and NtLAR expression but repressed NtDFR expression. More interestingly, NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptome-based WGCNA and confirmed by yeast two hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assay. Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted (pro)anthocyanin accumulation, while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols, but repressed NtDFR expression. These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and (pro)anthocyanin biosynthesis.
Graphical Abstract
NtMYB12 dually functions on
flavonol and proanthocyanin biogenesis via
physically binding to NtFLS and NtLAR promoter activating their expression and on (pro)anthocyanin biosynthesis via NtMYB12-NtWD40-NtbHLH (MBW) triplex activating NtDFR and NtANS expression. Requirement of NtMYB12 alone or MBW complex for the competition between flavonol and anthocyanin biosynthesis results in narcissus colorized petal traits.
Maximum correntropy self-organizing map for waveform classification
Authors: Shen Shi’an , Wei Xinjian , Wang Xiaokai , Chen Wenchao 
Published: February 2023

The self-organizing map (SOM) is a well-known unsupervised classification method for seismic facies analysis. The conventional SOM networks and their mutation methods are mostly based on the Euclidean distance measurement, which estimates the global similarity between the input data and weights of neuron. Such measurement is suitable for signals with a Gaussian distribution but may not work well for seismic data for which the dominant structural features (e.g., stratigraphic layers, faults, channels, and salt bodies) typically appear some specially distributed patterns. Therefore, we propose to modify the SOM by introducing a new distance measurement criterion based on the maximum correntropy. The correntropy is mainly used to measure the local similarity between variables, especially for non-Gaussian signals, and therefore is more suitable than the Euclidean distance to adaptively characterize the seismic structural similarities for seismic classification tasks. Validations on two synthetic data sets indicate that the proposed method yields more accurate classification results and is more robust to noise than the traditional SOM algorithm. Comparison experiments on a field data example also demonstrate that the proposed method can delineate the distribution of seismic facies more effectively and stably than the traditional one.
The determinants of the individual nestedness contribution in financial systems
Authors: Alexandre M. , Xavier F. , Silva T. , Rodrigues F. 
Published: February 2023

Abstract
Nestedness is one of the most pervasive and studied patterns observed in complex networks and refers to a hierarchical organization of the network. In this paper, we assess the determinants of the individual nestedness contribution (INC) in financial systems. To perform this task, we rely on data from two Brazilian financial networks: the bank-firm credit network and the interbank network. We computed the nestedness of the networks, as well as the INC for each node. The main determinants of the INC in the bank-firm network are the degree and the core number. In the interbank network, the INC of lending banks is mainly driven by their degree, while the INC of borrowing banks has not a clear main determinant.
Thermal performance curve of endurance running at high temperatures in deer mice
Authors: Eizenga Matthew R. , Flewwelling Luke D. , Warrier Tanisha , Scott Graham R. 
Published: February 2023

ABSTRACTThe impacts of warming temperatures associated with climate change on performance are poorly understood in most mammals. Thermal performance curves are a valuable means of examining the effects of temperature on performance traits, but they have rarely been used in endotherms. Here, we examined the thermal performance curve of endurance running capacity at high temperatures in the deer mouse (Peromyscus maniculatus). Endurance capacity was measured using an incremental speed test on a treadmill, and subcutaneous temperature in the abdominal region was measured as a proxy for body temperature (Tb). Endurance time at 20°C was repeatable but varied appreciably across individuals, and was unaffected by sex or body mass. Endurance capacity was maintained across a broad range of ambient temperatures (Ta) but was reduced above 35°C. Tb during running varied with Ta, and reductions in endurance were associated with Tb greater than 40°C when Ta was above 35°C. At the high Ta that limited endurance running capacity (but not at lower Ta), Tb tended to rise throughout running trials with increases in running speed. Metabolic and thermoregulatory measurements at rest showed that Tb, evaporative water loss and breathing frequency increased at Ta of 36°C and above. Therefore, the upper threshold temperatures at which endurance capacity is impaired are similar to those inducing heat responses at rest in this species. These findings help discern the mechanisms by which deer mice are impacted by warming temperatures, and provide a general approach for examining thermal breadth of performance in small mammals.
Accuracy and precision of contrast enhanced mammography versus MRI for predicting breast cancer size: how “good” are they really?
Authors: Taylor Donna Blanche , Burrows Sally , Dessauvagie Benjamin Frederik , Saunders Christobel Mary , Ives Angela 
Published: February 2023

Objective: To evaluate and compare the accuracy and precision of contrast-enhanced mammography (CEM) vs MRI to predict the size of biopsy-proven invasive breast cancer. Methods: Prospective study, 59 women with invasive breast cancer on needle biopsy underwent CEM and breast MRI. Two breast radiologists read each patient’s study, with access limited to one modality. CEM lesion size was measured using low-energy and recombined images and on MRI, the first post-contrast series. Extent of abnormality per quadrant was measured for multifocal lesions. Reference standards were size of largest invasive malignant lesion, invasive (PathInvasive) and whole (PathTotal). Pre-defined clinical concordance ±10 mm. Results: Mean patient age 56 years, 42 (71%) asymptomatic. Lesions were invasive ductal carcinoma 40 (68%) with ductal carcinoma in situ (31/40) in 78%, multifocal in 12 (20%). Median lesion size was 17 mm (invasive) and 27 mm (total), range (5–125 mm). Lin’s concordance correlation coefficients for PathTotal 0.75 (95% CI 0.6, 0.84) and 0.71 (95% CI 0.56, 0.82) for MRI and contrast-enhanced spectral mammography (CESM) respectively. Mean difference for total size, 3% underestimated and 4% overestimated, and for invasive 41% and 50% overestimate on MRI and CESM respectively. LOAs for PathTotal varied from 60% under to a 2.4 or almost threefold over estimation. MRI was concordant with PathTotal in 36 (64%) cases compared with 32 (57%) for CESM. Both modalities concordant in 26 (46%) cases respectively. Conclusion Neither CEM nor MRI have sufficient accuracy to direct changes in planned treatment without needle biopsy confirmation. Advances in knowledge: Despite small mean differences in lesion size estimates using CEM or MRI, the 95% limits of agreement do not meet clinically acceptable levels.
What and how do different stakeholders contribute to intervention development? A mixed methods study.
Authors: Racine Emmy , O Mahony Lauren , Riordan Fiona , Flynn Gráinne , Kearney Patricia M. , McHugh Sheena M. 
Published: February 2023

Background: UK Medical Research Council guidelines recommend end-user involvement in intervention development. There is limited evidence on the contributions of different end-users to this process. The aim of this Study Within A Trial (SWAT) was to identify and compare contributions from two groups of end-users - people with diabetes’ (PWD) and healthcare professionals’ (HCPs), during consensus meetings to inform an intervention to improve retinopathy screening uptake. Methods: A mixed method, explanatory sequential design comprising a survey and three semi-structured consensus meetings was used. PWD were randomly assigned to a PWD only or combined meeting. HCPs attended a HCP only or combined meeting, based on availability. In the survey, participants rated intervention proposals on acceptability and feasibility. Survey results informed the meeting topic guide. Transcripts were analysed deductively to compare feedback on intervention proposals, suggestions for new content, and contributions to the final intervention. Results: Overall, 13 PWD and 17 HCPs completed the survey, and 16 PWD and 15 HCPs attended meetings. For 31 of the 39 intervention proposals in the survey, there were differences (≥10%) between the proportion of HCPs and PWD who rated proposals as acceptable and/or feasible. End-user groups shared and unique concerns about proposals; both were concerned about informing but not scaring people when communicating risk, while concerns about resources were mostly unique to HCPs and concerns about privacy were mostly unique to PWD. Fewer suggestions for new intervention content from the combined meeting were integrated into the final intervention as they were not feasible for implementation in general practice. Participants contributed four new behaviour change techniques not present in the original proposals: goal setting (outcome), restructuring the physical environment, material incentive (behaviour) and punishment. Conclusions: Preferences for intervention content may differ across end-user groups, with feedback varying depending on whether end-users are involved simultaneously or separately.
Community paramedic outreach support for people experiencing homelessness
Authors: Taplin John , Dalgarno Dana , Smith Michele , Eggenberger Ty , Ghosh Sumantra Monty 
Published: February 2023

Objective:To evaluate the implementation phase of a community paramedic program focused on individuals experiencing homelessness (IEH) in Calgary, Alberta, Canada with a high prevalence of mental health and substance use disorders.Methods:A manual chart review was performed to characterise patients of the City Centre Team (CCT). A survey was performed of health professionals and partners for allied agencies that interacted with the CCT in the first 6 months of program implementation.Findings:There were 832 patient events involving 365 unique patients. Mental health and substance use disorders accounted for 75% of the events. The survey results were positive with 85% agreeing that the CCT reduced the reliance on emergency services.Conclusion:The community paramedics address the health and social needs of IEH while working in a multidisciplinary setting. The CCT is an innovative program that can inform future health service design in similar settings.
Specialist paramedics' perceptions of their transition to primary care
Authors: Lafitte Naomi , Mold Freda , Magnusson Carin 
Published: February 2023

Background: Many specialist paramedics are moving from the ambulance service to primary care. There is a lack of empirical literature regarding this transition. Aims: This study aims to improve support for specialist paramedics undertaking the transition by ascertaining factors that may hinder or facilitate the process. Methods: The study used semi-structured interviews with eight specialist paramedics working in primary care in England. An interpretative phenomenological analysis approach was used to analyse data, and this was informed by a review of theoretical and empirical literature. Findings: Facilitators and barriers to transition were found, along with information regarding the transition. Five facilitators were identified: previous clinical experience; mentorship; support; clear role parameters; and opportunity to develop. Four barriers were identified: role misunderstanding; changing scope of clinical practice; time pressures; and gaps in education and knowledge. Conclusion: This study contributes to a limited field of research by highlighting barriers and facilitators to the transition of specialist paramedics to primary care. Practical steps can be taken to smooth this transition.
Employing channel probing to derive end-of-life service margins for optical spectrum services
Authors: Kaeval Kaida , Slyne Frank , Troia Sebastian , Kenny Eoin , Grobe Klaus , Griesser Helmut , Kilper Daniel C. , Ruffini Marco , Pedreno-Manresa Jose-Juan , Patri Sai Kireet , Jervan Gert 
Published: February 2023

Optical spectrum as a service (OSaaS) spanning over multiple transparent optical network domains can significantly reduce the investment and operational costs of the end-to-end service. Based on the black-link approach, these services are empowered by reconfigurable transceivers and the emerging disaggregation trend in optical transport networks. This work investigates the accuracy aspects of the channel probing method used in generalized signal-to-noise ratio (GSNR)-based OSaaS characterization in terrestrial brownfield systems. OSaaS service margins to accommodate impacts from enabling neighboring channels and end-of-life channel loads are experimentally derived in a systematic lab study carried out in the Open Ireland testbed. The applicability of the lab-derived margins is then verified in the HEAnet production network using a 400 GHz wide OSaaS. Finally, the probing accuracy is tested by depleting the GSNR margin through power adjustments utilizing the same 400 GHz OSaaS in the HEAnet live network. A minimum of 0.92 dB and 1.46 dB of service margin allocation is recommended to accommodate the impacts of enabling neighboring channels and end-of-life channel loads. A further 0.6 dB of GSNR margin should be allocated to compensate for probing inaccuracies.
Graphene coated micro-channel fiber sensor based on localized surface plasmon resonance
Authors: Zhang Jingao , Yuan Jinhui , Qu Yuwei , Qiu Shi , Mei Chao , Zhou Xian , Yan Binbin , Wu Qiang , Wang Kuiru , Sang Xinzhu , Yu Chongxiu 
Published: February 2023

In this paper, a novel localized surface plasmon resonance (LSPR)-based micro-channel photonic crystal fiber (PCF) sensor is proposed. The LSPR-PCF sensor is designed with the three micro-channels, and the metal nanostraps and graphene are coated onto the PCF as the sensing elements. To realize high refractive index (RI) sensitivity, the influence of different plasmon materials, structure parameters, and graphene layers on the sensing performance of the LSPR-PCF sensor is investigated. The maximum wavelength sensitivity and amplitude sensitivity of the optimized LSPR-PCF sensor are up to 45800 nm/RIU and 1818RIU−1, respectively, when the RI changes from 1.35 to 1.42. Furthermore, the proposed LSPR-PCF sensor achieves the maximum figure of merit of 594.8, alone with a resolution of 2.18×10−6RIU. Because of its simple structure and excellent sensing performance, the proposed LSPR-PCF sensor has potential applications in biochemistry and environmental science.
Long-term visual prognosis and characteristics of recurrent retinal detachment after silicone oil removal
Authors: Park Wookyung , Kim Mirinae , Kim Rae Young , Kim Joo Young , Kwak Jae Hyuck , Park Young-Gun , Park Young-Hoon 
Published: February 2023

Purpose
Silicone oil (SO) is commonly used for tamponade purposes in retinal detachment (RD) surgery, but the long-term visual prognosis after removal of the oil, and in particular, what is known about the recurrence of RD after SO removal, remains unclear. The purpose of this study is to evaluate the long-term vision prognosis after SO removal, and to understand the frequency and characteristics of RD recurrence.

Methods
We retrospectively reviewed the medical charts of 1017 eyes of patients with a diagnosis of RD who had a pars plana vitrectomy with SO tamponade between January 2009 and December 2018. Best-corrected visual acuity (BCVA) was obatained before and after vitrectomy and also at the last visit. After SO removal, the group who showed improvement in visual acuity and the group who did not were compared. The anatomical results were compared between the group in which the retina was detached again after SO removal and the group in which the retina was not detached. To determine whether the duration of SO tamponade affects RD recurrence, further analysis was performed by dividing subgroups according to SO tamponade duration. RD recurrence, visual acuity, SO tamponade period were investigated.

Results
Mean follow-up period was 56.65 ± 72.02 months. An average SO tamponade period was 6.68 ± 11.39 months. The average logMAR BCVA was 1.75 ± 0.91 before SO injection, 1.60 ± 0.75 before SO removal and 1.29 ± 0.96 after the removal. After SO removal, 926 of the 1017 (91.1%) patients had well attached retina without recurrence. There was no significant difference in visual acuity before SO removal in re-detachment group compared to no re-detachment group, but visual acuity of re-detachment group was worse than no re-detachment group after SO removal (p<0.001). The SO tamponade period in the group with improved vision after SO removal was 5.09 ± 9.87 months, and the period was significantly shorter than the 9.09 ± 13.05 months in the group not showing vision recovery (p = 0.005). The occurrence of corneal opacity was significantly higher in the group with SO over 6 months, than those of the two groups with SO tamponade duration of less than 3 months and between 3 and 6 months (p = 0.038). The longest tamponade group showed the worst final vision after SO removal (p<0.001).

Conclusion
The prognosis for final vision is generally good when performing surgery using SO in RD, but considering the complications that arise after surgery, long-term retention of SO is not recommended and the timing of SO removal should be considered.
Development and cross-validation of a veterans mental health risk factor screen
Authors: Carlson Eve B. , Palmieri Patrick A. , Vogt Dawne , Macia Kathryn , Lindley Steven E. 
Published: February 2023

Background
VA primary care patients are routinely screened for current symptoms of PTSD, depression, and alcohol disorders, but many who screen positive do not engage in care. In addition to stigma about mental disorders and a high value on autonomy, some veterans may not seek care because of uncertainty about whether they need treatment to recover. A screen for mental health risk could provide an alternative motivation for patients to engage in care.

Method
Data from samples of veterans and traumatic injury survivors were analyzed to identify mental health risk factors that are characteristics of individuals or stressors or of post-trauma, post-deployment, or post-military service resources, experiences, or responses. Twelve risk factors were strongly related to PTSD (r > .50): current PTSD, depression, dissociation, negative thinking, and emotional lability symptoms, life stress, relationship stress, social constraints, and deployment experiences of a difficult environment, concerns about life and family, perceived threat, and moral injury. Items assessing each of these risk factors were selected and their validity to prospectively predict PTSD and/or depression 6 months later was assessed in a new sample of 232 VA primary care patients.

Results
Twelve items assessing dissociation, emotional lability, life stress, and moral injury correctly classified 86% of those who later had elevated PTSD and/or depression symptoms (sensitivity) and 75% of those whose later symptoms were not elevated (specificity). Performance was also very good for 110 veterans who identified as members of ethnic/racial minorities.

Conclusions
Mental health status was prospectively predicted in VA primary care patients with high accuracy using a screen that is brief, easy to administer, score, and interpret, and fits well into VA’s integrated primary care. When care is readily accessible, appealing to veterans, and not perceived as stigmatizing, information about mental health risk may result in higher rates of engagement than information about current mental disorder status.
Effect of an extension speech training program based on Chinese idioms in patients with post-stroke non-fluent aphasia: A randomized controlled trial
Authors: Pei Sun , Weiwei Li , Mengqin Zhang , Xiaojun He 
Published: February 2023

BackgroundChinese idioms have potential to act as preliminary training material in studies on post-stroke aphasia.ObjectiveTo explore an extension speech training program that takes Chinese idioms as context and expands them into characters, words, sentences and paragraphs and evaluate the effects of this program in patients with post-stroke non-fluent aphasia.MethodsThis was a randomized controlled trial. We recruited patients with post-stroke non-fluent aphasia from the Renmin Hospital of Wuhan University from January 2021 to January 2022. Participants were randomly assigned to group I and group II. Patients in group I had treatment with extension speech training based on Chinese idioms, and those in group II had treatment with conventional speech rehabilitation training. The training period in both groups was 40 min daily for 2 weeks.ResultsA total of 70 patients (group I, n = 34; and group II, n = 36) completed the trial and were analyzed according to protocol. There were no significant differences in baseline values between both groups. After intervention, the scores of oral expression, comprehension, and reading in the Aphasia Battery Of Chinese scale and the scores of the Comprehensive Activities of Daily Living questionnaire significantly improved in both groups (P <0.05), with group I benefiting more (P <0.05).ConclusionThis extension speech training program based on Chinese idioms can improve the language function and daily communication ability of the patients with post-stroke non-fluent aphasia.Trial registrationChinese Clinical Trial RegistryChiCTR2000031825.