bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spherical Nucleic Acid‐Amplified Digital Flow Cytometric Bead Assay for the Ultrasensitive Detection of Protein and Exosome†
Authors: Shi Jingjing , Sun Yuanyuan , Fan Wenjiao , Ren Wei , Liu Chenghui 
Published: April 2023

Comprehensive SummaryMost conventional digital bioassays rely on the use of fully‐sealed microchambers as independent units to compartmentalize the target molecules and the signal generation reaction, which require specialized equipment or proprietary reagents/consumables. Herein, we report a microchamber‐free and spherical nucleic acid (SNA)‐amplified digital flow cytometric bead assay (dFBA) for ultrasensitive protein and exosome analysis with simple workflows, easily accessible instruments/reagents, and high discriminating ability towards the fluorescence‐positive and fluorescence‐negative beads. In this dFBA, microbeads are employed as independent carriers to anchor the single target molecule‐initiated signal amplification reaction, avoiding the use of sealed droplets or microwell microchambers. Meanwhile, antibody‐functionalized SNAs (FSNAs) with a high density of DNA probes act as a bridge for efficiently amplified target‐to‐DNA signal conversion, which allows the use of DNA‐based rolling circle amplification (RCA) as the fluorescence signal amplification technique to quantify non‐nucleic acid targets. Even a single target‐induced on‐bead RCA and fluorescence enriching are sufficient to make the target‐loaded bead bright enough to be clearly discriminated from the negative ones just by use of a most common flow cytometer (FCM). This dFBA has successfully realized the digital analysis of ultralow levels of protein and exosome biomarkers, enlarging the toolbox of digital bioassays for clinical applications.
PROTOCOL: What is the effect of intergenerational activities on the wellbeing and mental health of older people?
Authors: Whear Rebecca , Campbell Fiona , Rogers Morwenna , Sutton Anthea , Robinson‐Carter Ellie , Sharpe Richard , Cohen Stuart , Fergy Ronald , Garside Ruth , Kneale Dylan , Melendez‐Torres G. J. , Thompson‐Coon Joanna 
Published: April 2023

AbstractThis is the protocol for a Campbell systematic review. The objectives are as follows: This systematic review will examine the impact of intergenerational interventions on the mental health and wellbeing of older people and will identify areas for future research as well as key messages for service commissioners.
Quality indicators in the endoscopic detection of gastric cancer
Authors: Namasivayam Vikneswaran , Uedo Noriya 
Published: April 2023

AbstractGastroscopy is the reference standard for the diagnosis of gastric cancer, but it is operator‐dependent and associated with missed gastric cancer. The proliferation of gastroscopic examinations, increasingly for the screening and detection of subtle premalignant lesions, has motivated scrutiny of quality in gastroscopy. The concept of a high‐quality endoscopic examination for the detection of superficial gastric neoplasia has been defined by expert guidelines to improve mucosal visualization, engender a systematic examination process and detect superficial neoplasia. This review discusses the evidence supporting the components of a high‐quality diagnostic gastroscopic examination in relation to the detection of gastric cancer, and their potential role as procedural quality indicators to drive a structured improvement in clinically meaningful outcomes.
Algeness—What do we know about this filler
Authors: Li Andraia R. , Taylor Brooke , Snyder Alan N. , Schlesinger Todd 
Published: April 2023

AbstractBackgroundDermal fillers are cosmetic injections that temporarily treat signs of aging. While hyaluronic acid (HA) fillers have been in forefront of the cosmetic industry, non‐HA‐based alternatives have increased in popularity over the past decade. Algeness is an agarose gel‐based dermal filler marketed as a natural and biodegradable alternative to HA. Available in four different concentrations, including 1%, 1.5%, 2.5%, and 3.5% agarose, Algeness has numerous applications ranging from lip augmentations to treatment of nasolabial folds and nonsurgical rhinoplasties. The structure of Algeness closely mimics that of the extracellular matrix and may be advantageous for mitigating migration. Algeness is also not readily digested by a specific human enzyme but is metabolized by macrophages of the reticuloendothelial system instead. These characteristics are thought to contribute to Algeness' durability and volumizing properties. The longevity of the filler directly correlates with the concentration of agarose incorporated, reportedly ranging from 3 to 15 months, depending on the formulation. Injection of Algeness differs from that of HA filler and a linear retrograde injection approach is recommended.ConclusionIn the limited studies performed thus far, Algeness has been shown to have comparable durability, patient satisfaction, and early adverse effects to HA fillers, particularly for treatment of the nasolabial folds. However, Algeness has not yet received Food and Drug Administration approval and is currently not available in US markets. Randomized clinical trials are currently underway to further evaluate its performance and safety.
Zebrafishcrocc2mutants exhibit divergent craniofacial shape, misregulated variability, and aberrant cartilage morphogenesis
Authors: Packard Mary C. , Gilbert Michelle C. , Tetrault Emily , Albertson R. Craig 
Published: April 2023

AbstractBackgroundPhenotypic variation is of paramount importance in development, evolution, and human health; however, the molecular mechanisms that influence organ shape and shape variability are not well understood. During craniofacial development, the behavior of skeletal precursors is regulated by both biochemical and environmental inputs, and the primary cilia play critical roles in transducing both types of signals. Here, we examine a gene that encodes a key constituent of the ciliary rootlets,crocc2, and its role in cartilage morphogenesis in larval zebrafish.ResultsGeometric morphometric analysis ofcrocc2mutants revealed altered craniofacial shapes and expanded variation. At the cellular level, we observed altered chondrocyte shapes and planar cell polarity across multiple stages incrocc2mutants. Notably, cellular defects were specific to areas that experience direct mechanical input. Cartilage cell number, apoptosis, and bone patterning were not affected incrocc2mutants.ConclusionsWhereas “regulatory” genes are widely implicated in patterning the craniofacial skeleton, genes that encode “structural” aspects of the cell are increasingly implicated in shaping the face. Our results addcrocc2to this list, and demonstrate that it affects craniofacial geometry and canalizes phenotypic variation. We propose that it does so via mechanosensing, possibly through the ciliary rootlet. If true, this would implicate a new organelle in skeletal development and evolution.
A novel double‐sampled square‐wave voltammetric technique combined with quadruple‐disk metallic‐glassy carbon electronic tongue for isotonic beverages profiling
Authors: Wójcik Szymon , Mirceski Valentin , Baś Bogusław , Jakubowska Małgorzata 
Published: April 2023

AbstractThis work demonstrates that quadruple‐disk electrodes with an innovative design, iridium‐platinum, platinum‐glassy carbon, and iridium‐glassy carbon can effectively function as a single sensor and act as a voltammetric electronic tongue in food profiling. The key strategy used was a novel technique called double‐sampled differential square‐wave voltammetry (DSSWV), which increased the resolution and enabled extraction of the full, hidden information available in the measuring signals. Four clustering validity indices, namely the Calinski – Harabasz index, Davies – Bouldin index, Silhouette index and gap statistics were employed to select the optimal sensor and methodology for interpreting the current samples. The best expected results were obtained using an iridium‐platinum electrode combined with overall cathodic potential modulation using the DSSWV technique. Principal component analysis and polar dendrogram confirmed the correct clustering of different isotonic beverages. However, it was also demonstrated that in some variants of measurement and signal interpretation, it was not possible to group the samples appropriately.
Ruddlesden‐Popper‐type perovskite Sr3Fe2O7−δ for enhanced thermochemical energy storage
Authors: Zhou Yue , Zhou Zijian , Sun Jian , Liu Lei , Luo Fei , Xu Guorong , Cao Xiangkun Elvis , Xu Minghou 
Published: April 2023

AbstractPerovskite has been considered a promising thermochemical energy storage material. Such materials can perform redox reactions reversibly under the control of oxygen partial pressure over a wide range of temperatures. Layered perovskites have been poorly studied as energy storage material, although their oxygen species exhibit good oxidation activity. In this work, Ruddlesden‐Popper‐type quasi‐2D perovskite Sr3Fe2O7‐δ and 3D perovskite SrFeO3‐δ were prepared for the testing of thermochemical energy storage properties. It was shown that the degree of reduction reaction for Sr3Fe2O7‐δ was much greater than that of SrFeO3‐δ, with change of non‐stoichiometry up to 0.79. The combined effect of thermodynamic parameters for samples on heat storage behavior was studied by Van't Hoff method. The reduction entropy of Sr3Fe2O7‐δ is much higher than that of SrFeO3‐δ, which explains the large promotion in the reaction degree of SrFeO3‐δ. The total reduction enthalpy of Sr3Fe2O7‐δ is about 2.8 times that of SrFeO3‐δ, with both reduction enthalpy and reaction entropy affecting the heat storage capacity. Sr3Fe2O7‐δ also has an attractive spectral absorption of 96.92% in the range of 300–2500 nm, which makes it advantageous in volumetric solar collector. Overall, Sr3Fe2O7‐δ offers improved performance in terms of thermochemical energy storage compared to SrFeO3‐δ.image
Contemporary research in coastal dunes and aeolian processes
Authors: Farrell Eugene J. , Delgado Fernandez Irene , Smyth Thomas , Li Bailiang , Swann Christy 
Published: April 2023

AbstractCoastal dunes are found along the sandy coasts of oceans, seas, and large lakes all around the world. They are dynamic landforms that evolve along complex morphological and biological continua in response to a range on controls linked to climate, sea level, sediment movement, vegetation cover, and land use. By collating research across the full spectrum of processes shaping different types and sizes of dunes and smaller aeolian bedforms, special issues can aid researchers to identify new research directions and methods emerging from the discipline. This editorial summarizes the 25 contributions to the special issue Coastal dunes: links between aeolian processes and landform dynamics. We grouped the contributions into four broad themes: (1) long‐term dune evolution, (2) short‐term aeolian transport, (3) research methods, and (4) coastal dune management. Contributions to the special issue demonstrate that research interest in coastal dunes, and particularly the impacts of human interventions on dunes, continues to grow. It also shows how aeolian research on coastal dunes covers a range of temporal and spatial scales, from ripple dynamics and instantaneous airflow‐transport processes to dune field evolution with rising sea levels and large‐scale dune stage shifts. We highlight potential avenues for future research including vegetation roughness characteristics and their effect on wind flow and sediment transport, the challenges of upscaling short‐ and small‐scale results to larger and longer spatiotemporal scales, and the study of both natural and managed dune landscapes.
Effects of Exposure to Cadmium, Microplastics, and Their Mixture on Survival, Growth, Feeding, and Life History of Daphnia magna
Authors: Zink Lauren , Shearer Anna Y. , Wiseman Steve , Pyle Gregory G. 
Published: April 2023

Abstract
There is concern that microplastics can act as a vector for cadmium (Cd), altering the bioavailability and subsequent toxicity of Cd to ecologically important species such as Daphnia magna. The toxicity of Cd to D. magna has been well described; however, what is not known, and what the present study addresses, was how the addition of polyethylene microplastic altered Cd toxicity. Using high-throughput feeding assays and size assessments, the present study quantified effects of exposure to Cd, microplastic, or their mixture on daphnids from neonate to adult. Exposure to Cd inhibited feeding efficiency, while exposure to microplastic inhibited growth rates of juveniles. Daphnia magna coexposed to Cd and microplastic showed significant decreases in both feeding and prereproductive growth rate. There were no differences in life-history traits across any treatments. The alterations of feeding and growth while maintaining reproductive endpoints (time to first brood, reproductive frequency, the number of neonates released at each reproductive event, and the size of neonates produced) might be the result of a shift in energy allocation away from somatic growth, allowing individuals to maintain reproductive output despite lower nutritional reserves. Our findings suggest that cocontamination of microplastic and Cd has additive effects on feeding and growth rates, resulting in a greater energy allocation shift. Environ Toxicol Chem 2023;42:1401–1408. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Effects of Nitrate and Conductivity on Embryo-Larval Fathead Minnows
Authors: Edwards Thea M. , Lamm Daniel J. , Harvey Joel J. 
Published: April 2023

Abstract
Nitrate concentrations have been rising in surface waters over the last century and now frequently exceed drinking water standards and environmental safety benchmarks globally. Health-wise, these trends are concerning because nitrate has been shown to disrupt endocrine function and developmental outcomes. The present study investigated potential sublethal effects of nitrate on developing fathead minnows. Fish were exposed from fertilization through 21 days postfertilization (dpf) to environmentally relevant concentrations of nitrate (0, 2, 5, 10, 25, or 100 mg/L NO3-N as NaNO3). Nitrate effects on hatch timing, heart rate and rhythm at 3 dpf, growth through 21 dpf, swim bladder inflation timing and size, scoliosis, pericardial edema, and mortality were assessed. Because adding NaNO3 increases water conductivity, two conductivity controls were included to match the ionic strength of the 10- and 100-mg/L NO3-N treatments. Increasing nitrate delayed posterior swim bladder (PSB) inflation in a dose-dependent manner, with possible inhibition of anterior swim bladder (ASB) inflation at higher doses, although nitrate did not affect swim bladder size. Conversely, nitrate did not affect hatch timing or cardiac endpoints at 3 dpf or induce pericardial edema or scoliosis, although there was a noted brood effect on these latter defects. As was observed with increasing nitrate, higher ion concentrations in the conductivity controls caused dose-dependent increases in fish body size at 21 dpf. Increased ionic strength also hastened ASB inflation independently of nitrate. As in other published studies, the observed delay in PSB inflation suggests that nitrate disrupts the thyroid axis and warrants further investigation. In addition, the present study supports the need for conductivity controls in nitrate toxicity studies to distinguish nitrate-specific effects. Environ Toxicol Chem 2023;42:1529–1541. Published 2023. This article is a U.S. Government work and is in the public domain in the USA.
Spatiotemporal single‐cell transcriptomic profiling reveals inflammatory cell states in a mouse model of diffuse alveolar damage
Authors: Su Duo , Jiao Zhouguang , Li Sha , Yue Liya , Li Cuidan , Deng Mengyun , Hu Lingfei , Dai Lupeng , Gao Bo , Wang Jinglin , Zhang Hanchen , Xiao Haihua , Chen Fei , Yang Huiying , Zhou Dongsheng 
Published: April 2023

AbstractDiffuse alveolar damage (DAD) triggers neutrophilic inflammation in damaged tissues of the lung, but little is known about the distinct roles of tissue structural cells in modulating the recruitment of neutrophils to damaged areas. Here, by combining single‐cell and spatial transcriptomics, and using quantitative assays, we systematically analyze inflammatory cell states in a mouse model of DAD‐induced neutrophilic inflammation after aerosolized intratracheal inoculation with ricin toxin. We show that homeostatic resident fibroblasts switch to a hyper‐inflammatory state, and the subsequent occurrence of a CXCL1‐CXCR2 chemokine axis between activated fibroblasts (AFib) as the signal sender and neutrophils as the signal receiver triggers further neutrophil recruitment. We also identify an anatomically localized inflamed niche (characterized by a close‐knit spatial intercellular contact between recruited neutrophils and AFib) in peribronchial regions that facilitate the pulmonary inflammation outbreak. Our findings identify an intricate interplay between hyper‐inflammatory fibroblasts and neutrophils and provide an overarching profile of dynamically changing inflammatory microenvironments during DAD progression.
Enzymatic reactions towards aldehydes: An overview
Authors: Schober Lukas , Dobiašová Hana , Jurkaš Valentina , Parmeggiani Fabio , Rudroff Florian , Winkler Margit 
Published: April 2023

AbstractMany aldehydes are volatile compounds with distinct and characteristic olfactory properties. The aldehydic functional group is reactive and, as such, an invaluable chemical multi‐tool to make all sorts of products. Owing to the reactivity, the selective synthesis of aldehydic is a challenging task. Nature has evolved a number of enzymatic reactions to produce aldehydes, and this review provides an overview of aldehyde‐forming reactions in biological systems and beyond. Whereas some of these biotransformations are still in their infancy in terms of synthetic applicability, others are developed to an extent that allows their implementation as industrial biocatalysts.
Climate Change Risks to Freshwater Subsistence Fisheries in Arctic Alaska: Insights and Uncertainty from Broad Whitefish Coregonus nasus
Authors: Leppi Jason C , Rinella Daniel J , Wipfli Mark S , Liljedahl Anna K , Seitz Andrew C , Falke Jeffrey A 
Published: April 2023

Abstract
Arctic freshwater ecosystems and fish populations are largely shaped by seasonal and long-term watershed hydrology. In this paper, we hypothesize how changing air temperature and precipitation will alter freeze and thaw processes, hydrology, and instream habitat to assess potential indirect effects, such as the change to the foraging and behavioral ecology, on Arctic fishes, using Broad Whitefish Coregonus nasus as an indicator species. Climate change is expected to continue to alter hydrologic pathways, flow regimes, and, therefore, habitat suitability, connectivity, and availability for fishes. Warming and lengthening of the growing season will likely increase fish growth rates; however, the exceedance of threshold stream temperatures will likely increase physiological stress and alter life histories. We expect these changes to have mixed effects on Arctic subsistence fishes and fisheries. Management and conservation approaches focused on preserving the processes that create heterogeneity in aquatic habitats, genes, and communities will help maintain the resilience of Broad Whitefish and other important subsistence fisheries. Long-term effects are uncertain, so filling scientific knowledge gaps, such as identifying important habitats or increasing knowledge of abiotic variables in priority watersheds, is key to understanding and potentially mitigating likely impacts to Arctic fishes in a rapidly changing landscape.
Deconstructive versus reconstructive approach to internal carotid artery blowout in radiated nasopharyngeal carcinoma: A tertiary center experience and systematic review
Authors: Xu Xinni , Gopinathan Anil , Ong Yew Kwang , Thye Thong Mark Kim , Loh Woei Shyang , Loh Kwok Seng 
Published: April 2023

AbstractThe objective of this study was to compare the outcomes of parent artery occlusion (PAO) versus stent‐assisted reconstruction in radiated nasopharyngeal carcinoma (NPC) patients with internal carotid artery (ICA) blowouts. A retrospective review from our institution (2011–2021) and systematic review of Pubmed and Embase (1995–2022) was performed. Twenty‐eight eligible studies were identified. Eighty‐six PAOs and 37 stent‐assisted reconstructions were analyzed, including 11 PAOs and 5 stents from our institution. Stents were associated with significantly higher incidence of overall re‐bleeding (16.2% [95% CI 7.4–31.9] vs. 4.6% [95% CI 1.3–13.5], p = 0.047), delayed stroke (5.4% [95% CI 1.3–19.4] vs. 0%, p = 0.034) and reduced median survival (7.1 [95% CI 3.8–14.0] months vs. 29.0 [95% CI 9.4–63.4] months, p = 0.017) compared to PAO. There were no significant differences in terms of overall stroke, infection, extruded/migrated foreign body, and peri‐procedure death. PAO is preferred over reconstructive treatment in patients with adequate collateral circulation.
Investigation of heat transfer augmentation in a horizontal tube using different rectangular cut ring inserts
Authors: Khudhur Dhamyaa S. , Kassim Muna S. , Jasim Hadeel Abdulhadi 
Published: April 2023

AbstractExperimental and computational investigations have studied the heat transfer, friction factor, and enhancement of heat transfer in a horizontal tube equipped with rectangular cut ring inserts and different diameter ratios (D/d) and pitch‐to‐tube diameter ratios (p/dt). In the present study, air having a Reynolds no. range of 6700–20,100 was used as a working fluid. Three diameter ratios (D/d) were considered experimentally and numerically as 1.2, 1.25, and 1.3, and the pitch‐to‐tube diameter ratio (p/dt) was (1, 0.625, and 0.5). Air was forced as working fluid through the tube and a uniform heat flux of 2000, 3500, and 5000 W/m2 was applied through the tube's exterior surface. On the basis of the turbulence model k–ɛ with various parameters, three‐dimensional numerical simulations using the ANSYS Fluent software 17.2 were investigated. Under the same working conditions, the results manifested a higher heat transfer rate and friction factor as compared to the plain tube. The results evinced that the Nusselt number for a horizontal tube equipped with rectangular cut ring inserts having various pitch ratios and diameter ratios is discovered to be higher than that for the plain tube. With the increased ring spacing, the overall improvement in heat transfer occurred. And, with a rise in Re, the total enhancement ratio decreased. Consequently, the greatest overall improvement attained was 38% at Reynolds number (Re = 12,860) with the pitch ratio (p/dt = 1). The three diameter ratios (D/d) of 1.3, 1.25, and 1.2 gives in this study the average thermal performance factor in the value of 1.6, 1.5, and 1.4, respectively. Using the Nusselt number and friction factor, the results are correlated as a function of the Reynolds number, diameter ratio, and pitch ratio.