bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reproducibility and intratumoral heterogeneity of the PAM50 breast cancer assay
Authors: Hurson Amber N. , Hamilton Alina M. , Olsson Linnea T. , Kirk Erin L. , Sherman Mark E. , Calhoun Benjamin C. , Geradts Joseph , Troester Melissa A. 
Published: March 2023

Abstract
Background
The PAM50 assay is used routinely in clinical practice to determine breast cancer prognosis and management; however, research assessing how technical variation and intratumoral heterogeneity contribute to misclassification and reproducibility of these tests is limited.

Methods
We evaluated the impact of intratumoral heterogeneity on the reproducibility of results for the PAM50 assay by testing RNA extracted from formalin-fixed paraffin embedded breast cancer blocks sampled at distinct spatial locations. Samples were classified according to intrinsic subtype (Luminal A, Luminal B, HER2-enriched, Basal-like, or Normal-like) and risk of recurrence with proliferation score (ROR-P, high, medium, or low). Intratumoral heterogeneity and technical reproducibility (replicate assays on the same RNA) were assessed as percent categorical agreement between paired intratumoral and replicate samples. Euclidean distances between samples, calculated across the PAM50 genes and the ROR-P score, were compared for concordant vs. discordant samples.

Results
Technical replicates (N = 144) achieved 93% agreement for ROR-P group and 90% agreement on PAM50 subtype. For spatially distinct biological replicates (N = 40 intratumoral replicates), agreement was lower (81% for ROR-P and 76% for PAM50 subtype). The Euclidean distances between discordant technical replicates were bimodal, with discordant samples showing higher Euclidian distance and biologic heterogeneity.

Conclusion
The PAM50 assay achieved very high technical reproducibility for breast cancer subtyping and ROR-P, but intratumoral heterogeneity is revealed by the assay in a small proportion of cases.
Short term outcome of myocarditis and pericarditis following COVID-19 vaccines: a cardiac magnetic resonance imaging study
Authors: Galea Nicola , Cundari Giulia , Di Dedda Emanuele , Chimenti Cristina , Aquaro Giovanni Donato , Barison Andrea , Cau Riccardo , Di Cesare Ernesto , Di Renzi Paolo , Esposito Antonio , Faletti Riccardo , Gatti Marco , Liguori Carlo , Lovato Luigi , Mantini Cesare , Monti Caterina Beatrice , Palmisano Anna , Pradella Silvia , Ricci Fabrizio , Saba Luca , Secchi Francesco , Catalano Carlo , Francone Marco 
Published: March 2023

AbstractTo evaluate clinical and cardiac magnetic resonance (CMR) short-term follow-up (FU) in patients with vaccine-associated myocarditis, pericarditis or myo-pericarditis (VAMP) following COVID-19 vaccination. We retrospectively analyzed 44 patients (2 women, mean age: 31.7 ± 15.1 years) with clinical and CMR manifestations of VAMP, recruited from 13 large tertiary national centers. Inclusion criteria were troponin raise, interval between the last vaccination dose and onset of symptoms < 25 days and symptoms-to-CMR < 20 days. 29/44 patients underwent a short-term FU-CMR with a median time of 3.3 months. Ventricular volumes and CMR findings of cardiac injury were collected in all exams. Mean interval between the last vaccination dose and the onset of symptoms was 6.2 ± 5.6 days. 30/44 patients received a vaccination with Comirnaty, 12/44 with Spikevax, 1/44 with Vaxzevria and 1/44 with Janssen (18 after the first dose of vaccine, 20 after the second and 6 after the “booster” dose). Chest pain was the most frequent symptom (41/44), followed by fever (29/44), myalgia (17/44), dyspnea (13/44) and palpitations (11/44). At baseline, left ventricular ejection fraction (LV-EF) was reduced in 7 patients; wall motion abnormalities have been detected in 10. Myocardial edema was found in 35 (79.5%) and LGE in 40 (90.9%) patients. Clinical FU revealed symptoms persistence in 8/44 patients. At FU-CMR, LV-EF was reduced only in 2 patients, myocardial edema was present in 8/29 patients and LGE in 26/29. VAMPs appear to have a mild clinical presentation, with self-limiting course and resolution of CMR signs of active inflammation at short-term follow-up in most of the cases.
Invariant manifolds near $$L_1$$ and $$L_2$$ in the quasi-bicircular problem
Authors: Rosales José J. , Jorba Àngel , Jorba-Cuscó Marc 
Published: March 2023

AbstractThe quasi-bicircular problem (QBCP) is a periodic time-dependent perturbation of the Earth–Moon restricted three-body problem (RTBP) that accounts for the effect of the Sun. It is based on using a periodic solution of the Earth–Moon–Sun three-body problem to write the equations of motion of the infinitesimal particle. The paper focuses on the dynamics near the $$L_1$$

L
1

and $$L_2$$

L
2

points of the Earth–Moon system in the QBCP. By means of a periodic time-dependent reduction to the center manifold, we show the existence of two families of quasi-periodic Lyapunov orbits around $$L_1$$

L
1

(resp. $$L_2$$

L
2

) with two basic frequencies. The first of these two families is contained in the Earth–Moon plane and undergoes an out-of-plane (quasi-periodic) pitchfork bifurcation giving rise to a family of quasi-periodic Halo orbits. This analysis is complemented with the continuation of families of 2D tori. In particular, the planar and vertical Lyapunov families are continued, and their stability analyzed. Finally, examples of invariant manifolds associated with invariant 2D tori around the $$L_2$$

L
2

that pass close to the Earth are shown. This phenomenon is not observed in the RTBP and opens the room to direct transfers from the Earth to the Earth–Moon $$L_2$$

L
2

region.
Sensitivity analysis of polar orbiter motion to lunar viscoelastic tidal deformation
Authors: Hu Xuanyu , Stark Alexander , Dirkx Dominic , Hussmann Hauke , Fienga Agnès , Briaud Arthur , Mémin Anthony , Melini Daniele , Fayolle Marie , Rambaux Nicolas , Baguet Daniel , Oberst Jürgen 
Published: March 2023

AbstractWe investigate the impact of viscoelastic tidal deformation of the Moon on the motion of a polar orbiter. The dissipative effects in the Moon’s interior, i.e., tidal phase lags, are modeled as Fourier series sampled at given frequencies associated with linear combinations of Delaunay arguments, the fundamental parameters describing the lunar motion around the Earth and the Sun. We implement the tidal model to evaluate the temporal lunar gravity field and the induced perturbation on the orbiter. We validate the numerical scheme via a frequency analysis of the perturbed orbital motion. We show that, in the case of the Lunar Reconnaissance Orbiter at a low altitude of less than 200 km, the main lunar tides and hence the potential Love numbers around the monthly and some multiple frequencies are dynamically separable. The omission of those effects in practice introduces a position error at the level of a few decimeters within 10 days.
Shifting range in a stingless bee leads to pre-mating reproductive interference between species
Authors: Paul Grace , Bartels Louisa , Bueno Francisco Garcia Bulle , Law Genevieve , Heard Tim , Chapman Nadine , Buchmann Gabriele , Lim Julianne , Gloag Rosalyn 
Published: March 2023

AbstractHuman-induced shifts in species’ ranges can increase contact between closely related species and lead to reproductive interference. In Australia, climate change and trade in stingless bee colonies is increasing the range overlap of two cryptic species: Tetragonula carbonaria and T. hockingsi. To investigate reproductive interactions between these species, we validated a diagnostic-PCR test based on the mitochondrial gene COI to ID field specimens to species. We then assessed the likelihood of reproductive interference in four ways. First, we imaged the male genitalia of each species and found no evidence of reproductive character displacement. Second, we assessed species composition of mating aggregations in an area of sympatry (Southeast Queensland) and confirmed that some males join the mating aggregations of interspecific colonies. Third, we translocated T. hockingsi colonies into the southern range of T. carbonaria (Sydney) and tracked their ability to requeen. These translocated colonies attracted mating aggregations comprised almost entirely of interspecific males, but never formed hybrid colonies; instead, queens either mated with their brothers, or the colony failed to requeen at all. Finally, we presented T. carbonaria males with either conspecific or interspecific virgin queens and found that males attempted to mate only with their own species’ queens. In all, we conclude that reproductive barriers between these species are complete with respect to “short-range” mating cues, but not for “long-range” mate attraction cues. Our study highlights that hive movements can increase some forms of pre-mating reproductive interference between managed bee species, even where the species do not actually mate or hybridize.
Exploring the Risk of Resulting in Homicide and Suicide in Spanish Missing Person Cases
Authors: García-Barceló Néstor , Córcoles Miguel Ángel Alcázar , Menéndez Javier Revuelta , Woolnough Penny , Álvarez José Luis González 
Published: March 2023

AbstractThe study explores in depth the relationship between missing persons’ psychosocial and criminological characteristics/circumstances and violent-fatal outcomes (suicide and homicide). A relational analytical explicative study of 929 cases and controls was designed using a retrospective and stratified design. Data gathering was conducted through the content analysis of judicial and police information, as well as the development of psychological autopsy techniques and semi-structured interviews with the persons involved in the missing person cases including offenders in prison. Bivariate and multivariate statistical techniques were utilised for analyses. The findings showed that there are different risk and protective factors which can distinguish between good state of health, suicide, and homicide outcomes. This research entails implications for prevention and police risk assessment system.
Mega infrastructure projects and their contribution to sustainable development: the case of the Athens Metro
Authors: Mitoula Roido , Papavasileiou Angelos 
Published: March 2023

AbstractThis paper examines the critical role of Mega Infrastructure Projects in sustainable urban and peripheral development by presenting a Sustainable Infrastructure Serum Analysis supported by primary field research. In the Athens Metro case study, we examined the project's impact on sustainable development by analysing the opinions of the project's users. As a result, the Athens Metro serves as a case study to help us better understand sustainable infrastructure as a framework for green growth from the standpoint of society. The three pillars of sustainable development are inextricably linked. This study focuses on the social comprehension and acceptance of a Mega Infrastructure Project effects. We attempt to demonstrate the interdependence of the three pillars of sustainable development through public opinion responses to our research by developing a statistical model fed by public responses to a prototype questionnaire that we developed to support our research objectives. The study's findings highlighted the project's social acceptability and necessity by establishing a direct positive correlation between sustainability, society, the economy, and the environment from the standpoint of society.
Swelling Induced Twist in Hyperelastic Tubes Due to Spiral Patterned Biasing Fibers in the Cross Section
Authors: Demirkoparan Hasan , Pence Thomas J. 
Published: March 2023

AbstractSimple fiber reinforcing patterns can serve to guide deformations in specialized ways if the material experiences expansion due to some sort of swelling phenomenon. This occurs even when the only activation is via the material swelling itself; the fibers being a passive hyperelastic material embedded in a swellable hyperelastic matrix. Using anisotropic hyperelasticity where the usual incompressibility constraint is generalized to model swelling, we consider such fiber guided deformation in the context of a circular cylinder subject to uniform swelling. The material is taken to be transversely isotropic with a fiber pattern corresponding to helical spirals in each cross section. This paper extends previous work which had examined a traction free outer radius that expanded while the inner radius was held fixed. Because of the spiral pattern, the tube in these previous studies exhibited increasing twist as the swelling proceeded. The problem considered here takes both inner and outer radius as free surfaces, thus causing the amount of radial expansion itself to be unknown. It is found that the spiral fiber pattern again induces a twist, and that this pattern also influences the nature of the radial expansion.
Conformal Deformations of a Dilational Material Surface
Authors: Chen Yi-chao , Fried Eliot 
Published: March 2023

AbstractDilational materials, for which the angles between pairs of material fibers are preserved under deformations, are an important class of metamaterials. Although these materials are typically made by assembling discrete elemental building blocks in repeating patterns, continuum mechanics provides a powerful tool for exploring their macroscopic properties and response. We present an analysis of the constraint, the constitutive relation, and the equilibrium equations for homogeneous and isotropic dilational elastic material surfaces. We also describe the possibility of penalizing deviations from local area preservation to yield a framework for approximating isometric deformations of unstretchable elastic material surfaces.
Spatiotemporal analysis of lake chlorophyll-a with combined in situ and satellite data
Authors: Kallio K. , Malve O. , Siivola E. , Kervinen M. , Koponen S. , Lepistö A. , Lindfors A. , Laine M. 
Published: March 2023

Abstract
We estimated chlorophyll-a (Chl-a) concentration using various combinations of routine sampling, automatic station measurements, and MERIS satellite images. Our study site was the northern part of the large, shallow, mesotrophic Lake Pyhäjärvi located in southwestern Finland. Various combinations of measurements were interpolated spatiotemporally using a data fusion system (DFS) based on an ensemble Kalman filter and smoother algorithms. The estimated concentrations together with corresponding 68% confidence intervals are presented as time series at routine sampling and automated stations, as maps and as mean values over the EU Water Framework Directive monitoring period, to evaluate the efficiency of various monitoring methods. The mean Chl-a calculated with DFS in June–September was 6.5–7.5 µg/l, depending on the observations used as input. At the routine monitoring station where grab samples were used, the average uncertainty (standard deviation, SD) decreased from 2.7 to 1.6 µg/l when EO data were also included in the estimation. At the automatic station, located 0.9 km from the routine monitoring site, the SD was 0.7 µg/l. The SD of spatial mean concentration decreased from 6.7 to 2.9 µg/l when satellite observations were included in June–September, in addition to in situ monitoring data. This demonstrates the high value of the information derived from satellite observations. The conclusion is that the confidence of Chl-a monitoring could be increased by deploying spatially extensive measurements in the form of satellite imaging or transects conducted with flow-through sensors installed on a boat and spatiotemporal interpolation of the multisource data.
The future of employment revisited: how model selection affects digitization risks
Authors: Lorenz Hanno , Stephany Fabian , Kluge Jan 
Published: March 2023

AbstractThe uniqueness of human labour is at question in times of smart technologies. As computing power and data available increases, the discussion on technological unemployment reawakens. Prominently, Frey and Osborne (Technol Forecast Soc Change 114:254–280, 2017) estimated that half of US employment must be considered exposed to computerization within the next 20 years; followed by a series of papers expanding the research with information on heterogeneous job-specific tasks within the same jobs diminishing digitization potentials to only smaller fractions of workers at high risk. The main contribution of our work is to show that the diversity of previous findings regarding the degree of digitization is additionally driven by model selection. For our case study, we consult experts in machine learning and industry professionals on the susceptibility to digital technologies in the Austrian labour market. Our results indicate that, while clerical computer-based routine jobs are likely to change in the next decade, professional activities, such as the processing of complex information, are less prone to digital change.
Has the Russian invasion of Ukraine reinforced anti-globalization sentiment in Austria?
Authors: Gutmann Jerg , Pitlik Hans , Fronaschütz Andrea 
Published: March 2023

AbstractThe Russian invasion of Ukraine has caused disruptions in international trade and highlighted the dependency of small open economies in Europe on imports, especially of energy. These events may have changed Europeans’ attitude towards globalization. We study two waves of representative population surveys conducted in Austria, one right before the Russian invasion and the other two months later. Our unique dataset allows us to assess changes in the Austrian public’s attitudes towards globalization and import dependency as a short-term reaction to economic turbulences and geopolitical upheaval at the onset of war in Europe. We show that two months after the invasion, anti-globalization sentiment in general has not spread, but that people have become more concerned about strategic external dependencies, especially in energy imports, suggesting that citizens’ attitudes regarding globalization are differentiated.
Catalysing the monitoring and evaluation of Nationally Determined Contributions through North–South cooperation
Authors: Abanda F. H. , Chia E. L. , Enongene K. E. , Fobissie K. , Manjia M. B. , Pettang C. 
Published: March 2023

AbstractThe recent 6th Assessment Report by Intergovernmental Panel on Climate Change has been damning to the world. An overwhelming amount of evidence that Nationally Determined Contributions (NDCs) can contribute to stabilising or reversing the course of impacts of climate change is now common. Given the likely update of NDC measures compounded by their complexities and limited resources, it is imperative to adopt effective Monitoring & Evaluation (M&E) systems to ensure that NDCs achieve their planned objectives. Effective roll-out and M&E of NDCs require full participation from all actors in various countries. However, despite existing evidence that shows the developing countries are the most affected by climate change, the role of their researchers in climate change research is not known. Therefore, the need to investigate the global North–South disparities and develop an agenda for future research about NDCs is imperative. To address this gap, a systematic review was undertaken using appropriate terms in Web of Science, Dimensions and ScienceDirect to identify relevant literature. The analysis of the identified literature led to two main findings. Firstly, most studies about NDCs are conducted by global North research institutes and researchers with very little involvement of those from the global South. Secondly, there is a global paucity of research about M&E of NDCs measures. As a major recommendation, while countries should equitably contribute to rolling out NDC projects, research should play a key role and should be inclusive as possible representing voices from the global North and South.
Resistance QTLs controlling leaf and neck blast disease identified in a doubled haploid rice population
Authors: Korinsak Siripar , Sriprakhon Saengchai , Sirithunya Kanyanat , Sriwongchai Tanee , Wongsaprom Chanakarn , Plabpla Anucha , Vanavichit Apichart , Toojinda Theerayut 
Published: March 2023

AbstractOne of the biotic constraints in rice production worldwide is blast disease which can control by planting resistant varieties. To find out effective resistance, blast resistance quantitative trait loci (QTL) were mapped against 20 and 3 virulent isolates for leaf blast and neck blast, respectively, using 111 doubled haploid lines from the cross of IR64 and Azucena. QTLs associated with leaf blast were found on chromosomes 1, 2, 4, 7, 8, 10, 11, and 12 (%R2 = 3.6 – 64.3), while neck blast linked QTLs were identified on chromosomes 1, 6, 10, and 12 (%R2 = 6.4 – 22.6). The new QTLs were identified on chromosome 1; however, most QTLs were mapped in the vicinity of resistance genes in previous references. The genetic relationship of leaf and neck blast was explained by the coincidence of detected QTLs and positive value of pathogenicity correlation (r = 4.5 – 4.7). This study provides reliable QTLs locations that will benefit rice breeding programs to develop new cultivars containing durable and broad-spectrum resistance to leaf and neck blast disease.
Abortion in International Human Rights Law: Missed Opportunities in Manuela v El Salvador
Authors: Smyth Rebecca 
Published: March 2023

AbstractThe Inter-American Court of Human Rights’ judgment in Manuela and Others v El Salvador represents a missed opportunity for advancing abortion access and sexual and reproductive health and rights in international human rights law (IHRL). Even though this case is representative of the multiple human right violations arising from El Salvador’s complete criminalisation of abortion and active prosecution of those suspected of having had the procedure, the Court shied away from engaging in a critique of El Salvador’s abortion legislation. Instead, it focused on issues relating to pre-trial detention, due process, and medical confidentiality. Despite growing consensus in IHRL that abortion must be decriminalised at a minimum in certain circumstances; indications that the inter-American human rights system subscribes to this position; and extensive evidence that El Salvador’s abortion legislation is resulting in human rights violations, the Court failed to use this judgment to articulate a clear and assertive position on the need for abortion access to realise sexual and reproductive health and rights.
SonOpt: understanding the behaviour of bi-objective population-based optimisation algorithms through sound
Authors: Asonitis Tasos , Allmendinger Richard , Benatan Matt , Climent Ricardo 
Published: March 2023

AbstractWe present an extension of SonOpt, the first ever openly available tool for the sonification of bi-objective population-based optimisation algorithms. SonOpt has already introduced benefits on the understanding of algorithmic behaviour by proposing the use of sound as a medium for the process monitoring of bi-objective optimisation algorithms. The first edition of SonOpt utilised two different sonification paths to provide information on convergence, population diversity, recurrence of objective values across consecutive generations and the shape of the approximation set. The present extension provides further insight through the introduction of a third sonification path, which involves hypervolume contributions to facilitate the understanding of the relative importance of non-dominated solutions. Using a different sound generation approach than the existing ones, this newly proposed sonification path utilizes pitch deviations to highlight the distribution of hypervolume contributions across the approximation set. To demonstrate the benefits of SonOpt we compare the sonic results obtained from two popular population-based multi-objective optimisation algorithms, Non-Dominated Sorting Genetic Algorithm (NSGA-II) and Multi-Objective Evolutionary Algorithm based on Decomposition (MOEA/D), and use a Multi-objective Random Search (MRS) approach as a baseline. The three algorithms are applied to numerous test problems and showcase how sonification can reveal various aspects of the optimisation process that may not be obvious from visualisation alone. SonOpt is available for download athttps://github.com/tasos-a/SonOpt-2.0.