bluuebunny/crossref_metadata_2025_split huggingface dataset:
CoCl2-mimicked Hypoxia Induces the Assembly of Stress Granules
in Trophoblast Cells Via eIF2α Phosphorylation-dependent and -
Independent Pathways
Authors: Ma Chunling , Lv Qiulan , Ma Liang , Xing Baoxiang , Li Yan , Li Zhiyuan 
Published: September 2023

Introduction:
Hypoxia has been implicated in preeclampsia (PE) pathophysiology.
Stress granules (SGs) are present in the placenta of patients with PE.
However, the pathways that contribute to SG aggregation in PE remain poorly
understood.

Objective:
The objective of the current study is to investigate this issue.

Methods:
We first established an in vitro hypoxia model using human trophoblast cell
line HTR-8/SVneo treated with cobalt chloride (CoCl2). CCK8 assay and wound healing
assay were conducted to assess the viability and migration of HTR-8/SVneo cells after
exposure to CoCl2-mimicked hypoxia. SG component expression in HTR-8/SVneo cells
treated with CoCl2 alone, or in combination with indicated siRNAs was evaluated by
reverse transcription quantitative PCR (RT-qPCR), western blot and immunofluorescence
staining.

Results:
Our results found CoCl2-mimicked hypoxia inhibits the proliferation and
migration of HTR-8/SVneo cells. The treatment of CoCl2 can induce SG assembly in
HTR-8/Svneo cells. Mechanistically, both heme-regulated inhibitors (HRI) mediated
eukaryotic translation initiation factor (eIF)2α phosphorylation pathway and 4E binding
protein 1 (4EBP1) pathway are involved in SG formation under the stress of CoCl2-
mimicked hypoxia.

Conclusion:
Hypoxia-induced SGs in trophoblast cells might contribute to the etiology of
PE.
Available Assistive Technology Outcome Measures: Systematic Review
Authors: Borgnis Francesca , Desideri Lorenzo , Converti Rosa Maria , Salatino Claudia 
Published: September 2023

Background
The World Health Organization claimed that measuring outcomes is necessary to understand the benefits of assistive technology (AT) and create evidence-based policies and systems to ensure universal access to it. In clinical practice, there is an increasing need for standardized methods to track AT interventions using outcome assessments.

Objective
This review provides an overview of the available outcome measures that can be used at the follow-up stage of any AT intervention and integrated into daily clinical or service practice.

Methods
We systematically searched for original manuscripts regarding available and used AT outcome measures by searching for titles and abstracts in the PubMed, Scopus, and Web of Science databases up to March 2023.

Results
We analyzed 955 articles, of which 50 (5.2%) were included in the review. Within these, 53 instruments have been mentioned and used to provide an AT outcome assessment. The most widely used tool is the Quebec User Evaluation of Satisfaction with Assistive Technology, followed by the Psychosocial Impact of Assistive Technology Scale. Moreover, the identified measures addressed 8 AT outcome domains: functional efficacy, satisfaction, psychosocial impact, caregiver burden, quality of life, participation, confidence, and usability. The AT category Assistive products for activities and participation relating to personal mobility and transportation was the most involved in the reviewed articles.

Conclusions
Among the 53 cited instruments, only 17 (32%) scales were designed to evaluate specifically assistive devices. Moreover, 64% (34/53) of the instruments were only mentioned once to denote poor uniformity and concordance in the instruments to be used, limiting the possibility of comparing the results of studies. This work could represent a good guide for promoting the use of validated AT outcome measures in clinical practice that can be helpful to AT assessment teams in their everyday activities and the improvement of clinical practice.
Splink: Latest developments and applications
Authors: Lindsay Sam , Kennedy Ross , Hepworth Tom , Bond Andy 
Published: September 2023

Funded by ADR UK, a new data linking team at the Ministry of Justice set out to link administrative datasets across the justice space, for internal use and sharing with external researchers. To achieve this aim we sought a linkage implementation that was probabilistic, flexible, scalable and ideally open source.
Taking into account the tools available at the MoJ, existing open-source software (and paid alternatives) failed to meet our desired criteria. It was decided to develop a software package that builds on FastLink’s implementation in R of an Expectation-Maximisation algorithm to estimate a Fellegi-Sunter linkage model, adding a range of technical improvements, increased functionality and customisation options. Distributed computing offered by Spark could facilitate comparable linkage jobs that run on much larger datasets and much faster. Working with government data, accountability and transparency are vital, so the data and models are made accessible by a range of intuitive visualizations.
The Splink python package has been downloaded over 6 million times. This initially used Spark to deliver its superior performance, but Splink v3 caters for various SQL backends and more potential users. As we have made Splink more intuitive, more accessible, and more extensively documented we continue to receive feedback and contributions from around the world, driving further continuous development.
Through technical innovation and user-focused development, Splink has improved access to cutting-edge data linkage, and created groundbreaking research opportunities at MoJ and beyond. The team is grateful to ONS and other collaborators for testing and adopting these tools, and we will present some of the latest developments as well as examples of how Splink has been used worldwide.
Ethnic differences in reading and mathematical test performance in primary schools in England
Authors: Bessudnov Alexey 
Published: September 2023

ObjectivesThis study investigates ethnic differences in Key Stage 2 (KS2) reading and mathematical test scores among primary school pupils in state schools in England between 2007 and 2018. The aim is to assess the performance across ethnic categories and examine its evolution over the course of the study period.
MethodsThe analysis uses data from the National Pupil Database combining ethnicity information from school censuses with KS2 attainment data. KS2 reading and mathematical tests are taken by Year 6 pupils aged 10 to 11. Mean test scores are compared across ethnic categories, while the method of relative distribution is employed to evaluate performance in each ethnic category relative to the White British across the entire distribution of test scores in 2007 and 2018.
ResultsBetween 2007 and 2018, the reading and maths test scores of British Bangladeshi, Black African, and Pakistani pupils improved relative to the White British group. In 2018, British Bangladeshi and Black African pupils performed at a similar or slightly higher level compared to their White British peers. The advantage in test scores in the two higher performing categories, British Indian and Chinese, further increased. Attainment in the other White category remained similar to the White British group. The test scores for the Black Caribbean and Mixed White and Black Caribbean categories tended to be concentrated in the lower part of the distribution. In 2018, the proportion of mixed and non-White pupils remained largely constant throughout the reading test score distribution, while in maths, a higher proportion of mixed and non-White pupils were found among high achievers compared to other parts of the distribution.
ConclusionFrom 2007 to 2018, KS2 test performance improved relative to the White British group for some ethnic categories, while it did not for others. This paper proposes potential explanations for these differences, which are related to the volume and characteristics of immigration to England. Further empirical testing is required in future research to substantiate these explanations.
KEPRAKTISAN ENSIKLOPEDIA FAMILI RUTACEAE DI KEBUN RAYA BANUA UNTUK MELATIHKAN KETERAMPILAN BERPIKIR KRITIS MAHASISWA
Authors: Masrurah Elva , Kaspul Kaspul , Zaini Muhammad 
Published: September 2023

Education is an effort to develop individual abilities and character through various processes such as guidance, teaching, training, and interaction with the environment. 21st century learning emphasizes critical thinking skills and environmental approaches related to the phrases think globally and act locally through the development of encyclopedias at Kebun Raya Banua. An encyclopedia is a type of learning resource that contains descriptions containing knowledge and is arranged (alphabetically) or according to the scientific environment by collaborating on text and images. This study aims to describe the practicality of the Rutaceae family encyclopedia at Kebun Raya Banua. The research method used is the EDR (Educational Design Research) method with Tessmer's formative evaluation design. The one-to-one test subjects were three students and the small group test subjects were four students. The object of this research is the encyclopedia of the Rutaceae family. The results of the study show that the practicality of the content is categorized as good and the practicality of expectations is categorized as very good. This shows that the encyclopedia of the rutaceae family at Kebun Raya Banua is as expected interesting and easy to use to train students' critical thinking skills (practical). Kata kunci: ensiklopedia, famili rutaceae, kepraktisan, penelitian pengembangan
PRODUKSI DAN KARAKTERISASI ECO-ENZIM DARI LIMBAH ORGANIK DAPUR
Authors: Widiani Nurhaida , Novitasari Aulia 
Published: September 2023

Garbage is the main problem causing environmental problems which are dominated by organic waste. Management of organic waste must be carried out extensively, one of which is by making eco-enzymes. This study aimed to determine the production of eco-enzymes from kitchen organic waste and the characterization of eco-enzymes based on them. This type of research is an experiment with a qualitative approach. The sample used is a mixture of kitchen organic waste, namely the production and characterization of eco-enzymes which include pH, COD, BOD, TSS, and TDS. The research results obtained data on eco-enzyme production by mixing 3 components in a ratio of 1 : 3: 10 (1 part sugar, 3 parts organic kitchen waste. 10 parts water), then put into a closed container and stored in a cool, dry place, circulation air is good, and dark for 3 months. Eco-enzyme characterization showed a pH of 2.59, a TDS of 2840mg/L, a TSS of 4.46mg/L, a BOD of 60 mg/L, and a COD of 13100mg/L. Kata kunci: Produksi, Karakterisasi, Eco-enzim
Scoulerine promotes cytotoxicity and attenuates stemness in ovarian cancer by targeting PI3K/AKT/mTOR axis
Authors: Wang Fang , Zhang Yang , Pang Rui , Shi Shaohong , Wang Ran 
Published: September 2023

Abstract
In women, ovarian cancer is a common gynecological cancer associated with poor prognosis, reoccurrence and chemoresistance. Scoulerine, a benzylisoquinoline alkaloid, has been reported effective against several carcinomas. Thus, we investigated the impact of scoulerine on ovarian cancer cells (OVCAR3). Cell viability was assessed by MTT assay, migration was determined by Boyden Chamber assay, while the invasion was monitored by Boyden Chamber assay using the matrigel. The stemness properties of OVCAR3 cells were observed by tumorsphere assay. Epithelial to mesenchymal transition (EMT) and stemness-related protein markers were monitored by real-time PCR analysis and immunoblotting. Scoulerine inhibits the viability of OVCAR3 cells with the IC
50 observed at 10 µmol L–1 after 48 h treatment. Scoulerine inhibited the colony-forming ability, migration and invasiveness of OVCAR3 cells in a dose-dependent fashion. Scoulerine treatment also drastically reduced the spheroid-forming ability of OVCAR3 cells. The mesenchymal and stemness--related markers like N-cadherin, vimentin, CD-44, Oct-4, Sox-2 and Aldh1A1 were downregulated, whereas the epithelial markers like E-cadherin and CD-24 were upregulated in scoulerine-treated cells. The upstream PI3K/Akt/mTOR-axis was downregulated in scoulerine-treated cells. We concluded that scoulerine successfully perturbs the cancerous properties of OVCAR3 cells by targeting the PI3K/Akt/mTOR axis. In vivo studies revealed a substantial decrease in tumor mass and volume after scoulerine treatment. Furthermore, scoulerine treatment was found to decrease oxidative stress factors in ovarian cancer mice model. Scoulerine is a potential anticancer agent against ovarian cancer and can be considered as a lead molecule for this malignancy, provided further investigations are performed.
Xanthoxyletin blocks the RANK/RANKL signaling pathway to suppress the growth of human pancreatic cancer cells
Authors: Zhang Xin , Li Luming , Wu Yan 
Published: September 2023

Abstract
Xanthoxyletin is a vital plant-derived bioactive coumarin. It has been shown to exhibit anticancer effects against different human cancers. Nonetheless, the anticancer effects of xanthoxyletin against human pancreatic cancer cells have not been evaluated. Against this backdrop, the present study was designed to evaluate the anticancer effects of xanthoxyletin in human pancreatic cancer cells and to decipher the underlying molecular mechanisms. The results revealed a significant (p < 0.05) upregulation of receptor activator of NF-kappaB (RANK), receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG) in human pancreatic tissues and cell lines at both transcriptional and translational levels. The administration of pancreatic cancer cells with xanthoxyletin diminished the viability of Capan-2 cells in a concentration-dependent manner and led to a significant decline in RANK, RANKL, and OPG expression. Silencing of RANK and xanthoxyletin treatment declined the viability of Capan-2 pancreatic cancer cells via induction of apoptosis. However, pancreatic cancer cells overexpressing RANK could rescue the growth inhibitory effects. Collectively, xanthoxyletin targets the RANK/RANKL signaling pathway in pancreatic cancer cells to induce cell apoptosis and may prove to be an important lead molecule.
Formulation and optimization of polymeric nanoparticles loaded with riolozatrione: a promising nanoformulation with potential antiherpetic activity
Authors: Solís-Cruz Guadalupe Y. , Alvarez-Roman Rocío , Rivas-Galindo Verónica M. , Galindo-Rodríguez Sergio Arturo , Silva-Mares David A. , Marino-Martínez Iván A. , Escobar-Saucedo Magdalena , Pérez-López Luis A. 
Published: September 2023

Abstract
Riolozatrione (RZ) is a diterpenoid compound isolated from a dichloromethane extract of the Jatropha dioica root. This compound has been shown to possess moderate antiherpetic activity in vitro. However, because of the poor solubility of this compound in aqueous vehicles, generating a stable formulation for potential use in the treatment of infection is challenging. The aim of this work was to optimize and physio-chemically characterize Eudragit® L100-55-based polymeric nanoparticles (NPs) loaded with RZ (NPR) for in vitro antiherpetic application. The NPs formulation was initially optimized using the dichloromethane extract of J. dioica, the major component of which was RZ. The optimized NPR formulation was stable, with a size of 263 nm, polydispersity index < 0.2, the zeta potential of –37 mV, and RZ encapsulation efficiency of 89 %. The NPR showed sustained release of RZ for 48 h with release percentages of 95 and 97 % at neutral and slightly acidic pH, respectively. Regarding in vitro antiherpetic activity, the optimized NPR showed a selectivity index for HSV-1 of ≈16 and for HSV-2 of 13.
Design and synthesis of amino-substituted N-arylpiperidinyl-based inhibitors of the (immuno)proteasome
Authors: Gobec Martina , Obreza Aleš , Jukič Marko , Baumgartner Ana , Mihelčič Nja , Potočnik Špela , Virant Julija , Mlinarič Irena , Stanislav Raščan , Sosič Gobec Izidor 
Published: September 2023

Abstract
The constitutive proteasome and the immunoproteasome represent validated targets for pharmacological intervention in the context of various diseases, such as cancer, inflammation, and autoimmune diseases. The development of novel chemical scaffolds of non-peptidic nature, capable of inhibiting different catalytically active subunits of both isoforms, is a viable approach against these diseases. Such compounds are also useful as leads for the development of biochemical probes that enable the studies of the roles of both isoforms in various biological contexts. Here, we present a ligand-based computational design of (immuno)proteasome inhibitors, which resulted in the amino-substituted N-arylpiperidine-based compounds that can inhibit different subunits of the (immuno)proteasome in the low micromolar range. The compounds represent a useful starting point for further structure-activity relationship studies that will, hopefully, lead to non-peptidic compounds that could be used in pharmacological and biochemical studies of both proteasomes.
Improving Grinding Ball Lifespan and Efficiency Through Hardenability Modelling and Optimization
Authors: Aissat Sahraoui , Safa Ali 
Published: September 2023

Abstract
Grinding balls are spherical or cylindrical components used in grinding and milling operations to reduce the size of particles and achieve a finer product. They are made of high chromium white cast iron (HCWCI) and used in a variety of industrial processes. The efficiency of the grinding process is heavily influenced by the properties of the grinding balls, including their composition, size, and hardness. As such, there is ongoing research and development to improve the performance and durability of grinding balls, with the aim of countering the extreme conditions of wear and impact that cause a reduction in their lifespan. This study involved austenitizing balls with diameters of 50 mm and 70 mm at temperatures of 950°C and 1050°C, followed by quenching using both oil and compressed air. By exploiting the experimental HRC hardness results obtained in this work, the study aims to find a mathematical model relating the response (hardenability) to the main effects (austenitization temperature, quenching medium, and diameter balls) and their interactions. Analysis of variance (ANOVA) was used to establish the statistical significance parameters and an optimization of response by the best sub-models method and by the desirability function is realized in the second part of this work. It seems that the austenitization temperature and the size of the balls have a stronger impact on the hardenability of the balls than the cooling rate (quenching medium) by reducing the hardness difference between the surface and the medium of the ball to minimal values.
Effect of Rise in Temperature (250°C) on the Physico-Mechanical Properties of Rubber Mortars
Authors: Assia Aidoud , Messaouda Boutahir Born Bencheikh , Salima Boukour , Ouassila Bahloul 
Published: September 2023

Abstract
The recovery and use of waste in the field of civil engineering, particularly in construction materials, is one of the most prominent solutions for preserving the environment. In order to evaluate the results obtained, it is necessary to study the evolution of the properties of these new materials in the different environments where they can live or be exposed, and why not develop an effective method of treatment of such materials for the possibility of their use even in the field of precast concrete. The objective of this work is to study the effect of the increase in temperature as a living environment or as a treatment on the physico-mechanical properties of a crumbled mortar, a potential source of many environmental and economic problems. Hence the screening and the possibility of using these new mortars with sufficient physico-mechanical properties for masonry and why not for prefabrication. The formulation of the mixtures based on the substitution of dune sand by crumb rubber, at different weight contents 10, 20 and 30%. Consequently, prismatic specimens (4x4x16) cm3 subjected to the temperature (250°C), with a speed of 2°C/min followed by a one-hour plateau at the target temperature then cooling to the ambient temperature. The results obtained show that the maximum mass loss is 5% for 30% substitution and that for 10% substitution the absorption by total immersion decreased by more than two thirds and the porosity accessible to water decreased by more than half. The compressive strength increases by 8.9% for 10% substitution and the minimum decrease in tensile strength by bending is at the same substitution of an order of 26.9%. Using the analysis of variance, the influence of the substitution of dune sand by rubber crumbs and of the rise in temperature to 250°C on the behavior of the mortar acquired. Patterns developed by response surface methodology were significant for all p-value substitutions <5%. The results of the numerical optimization showed that the best mixture could obtained by replacing 30% of dune sand with rubber crumb and subjecting the hardened mortar obtained from this mixture to the temperature of 135°C.
Electrotechnical Tools and Computer Image Analysis in Assessing the Quality of Maize Grain During Storage
Authors: Szwedziak Katarzyna , Dolezal Petr , Tabor Sylwester , Ogrodniczek Jacek 
Published: September 2023

Abstract
The study of qualitative characteristics is becoming increasingly important due to determination of the purchase price and further use of seeds. An important problem of the modern sustainable agriculture is the production of seeds and products with appropriate quality parameters. The research carried out so far proves that the technology of harvesting, transport, and drying conditions as well as storage have an impact on the quality of seeds, determining their usefulness for the industry. The smallest irregularities can cause irreversible changes and significantly reduce the technological value of seeds and their processing products. The use of tools in the field of supporting electrical engineering enables detection and highlighting of image elements so that it becomes readable to the human eye. The aim of the research was to develop technology for evaluating grain in storage using electrotechnical tools and computer techniques.