bluuebunny/crossref_metadata_2025_split huggingface dataset:
C-terminal amides mark proteins for degradation via SCF/FBXO31
Authors: Muhar Matthias , Farnung Jakob , Hofmann Raphael , Cernakova Martina , Sidiropoulos Nikolaos D. , Bode Jeffrey W. , Corn Jacob E. 
Published: June 2023

AbstractDuring normal cellular homeostasis unfolded and mis-localized proteins are recognized and removed, preventing the build-up of toxic byproducts1. When protein homeostasis is perturbed during aging, neurodegeneration or cellular stress, proteins can accumulate several forms of chemical damage through reactive metabolites2, 3. Such modifications have been proposed to trigger the selective removal of chemically marked proteins3–6;however, discovering modifications sufficient to induce protein degradation has remained challenging. Using a semi-synthetic chemical biology approach coupled to cellular assays, we found that C-terminal amide-bearing proteins (CTAPs) are rapidly cleared from human cells. A CRISPR screen identified the SCF/FBXO31 ubiquitin ligase as a reader of C-terminal amides, which ubiquitylates CTAPs for subsequent proteasomal degradation. A conserved binding pocket enables FBXO31 to bind almost any C-terminal peptide bearing an amide while retaining exquisite selectivity over non-modified clients. This mechanism facilitates binding and turnover of endogenous CTAPs that are formed following oxidative stress. A dominant human mutation found in neurodevelopmental disorders switches CTAP recognition, such that non-amidated neosubstrates are now degraded and FBXO31 becomes markedly toxic. We propose that CTAPs may represent the vanguard of a largely unexplored class of modified amino acid degrons that could provide a general strategy for selective yet broad surveillance of chemically damaged proteins.
Complete zooplankton size spectra re-constructed from « in situ » imaging and Multinet data in the global ocean
Authors: Soviadan Yawouvi Dodji , Dugenne Mathilde , Drago Laetitia , Biard Tristan , Trudnowska Emilia , Lombard Fabien , Romagnan Jean-Baptiste , Jamet Jean-Louis , Kiko Rainer , Gorsky Gabriel , Stemmann Lars 
Published: June 2023

AbstractPlankton size spectra are important indicators of the ecosystem state, as they illustrate the quantity of organisms available for higher marine food web and reflect multiple size-dependent processes. Yet, such measurements are typically biased by the available sampling methods, either disrupting fragile organisms or lacking good resolution (in size and/or time and space). In this study, we combined two of the most common approaches to measure zooplankton Normalized Biomass/Biovolume Size Spectra (NBSS) to calculate a complete zooplankton distribution for organisms larger than 1 mm. The reconstructed NBSS slopes appeared steeper and closer to those measured by the UVP5 (+7.6%) and flatter than those of the Multinet (- 20%) particularly in tropics and temperate latitudes. The overall gain in polar biomass was relatively small for reconstructed biomass compared to bulk estimates from Multinet (+0.24 mgC/m3 or +4.25%) and high from the UVP5 (+2.0 mgC/m3 or +53%). In contrast, in the tropical and temperate ecosystems, the gain in biomass was small for UVP5 (+0.67 mgC/m3 or +30.44% and +0.74 mgC/m3 or +19.59% respectively) and high for Multinet (+1.66 mgC/m3 or +136% and +3.4 mgC/m3 or +309% respectively). Given these differences, we suggest here to combinein situimaging sensors and net data in any comprehensive study exploring key living players in the ocean ecosystem and their contributions to the biological pump.
Strength of Statistical Evidence for the Efficacy of Cancer Drugs: A Bayesian Re-Analysis of Randomized Trials Supporting FDA Approval
Authors: Pittelkow Merle-Marie , Linde Maximilian , de Vries Ymkje Anna , Hemkens Lars G. , Schmitt Andreas M. , Meijer Rob R. , van Ravenzwaaij Don 
Published: June 2023

ABSTRACTObjectiveTo quantify the strength of statistical evidence of randomised controlled trials (RCTs) for novel cancer drugs approved by the Food and Drug Administration (FDA) in the last two decades.Study Design and SettingWe used data on overall survival (OS), progression-free survival (PFS), and tumour response (TR) for novel cancer drugs approved for the first time by the FDA between January 2000 and December 2020. We assessed strength of statistical evidence by calculating Bayes Factors (BFs) for all available endpoints, and we pooled evidence using Bayesian fixed-effect meta-analysis for indications approved based on two RCTs. Strength of statistical evidence was compared between endpoints, approval pathways, lines of treatment, and types of cancer.ResultsWe analysed the available data from 82 RCTs corresponding to 68 indications supported by a single RCT and seven indications supported by two RCTs. Median strength of statistical evidence was ambiguous for OS (BF= 1.9; IQR 0.5-14.5), and strong for PFS (BF= 24,767.8; IQR 109.0-7.3*106) and TR (BF= 113.9; IQR 3.0-547,100). Overall, 44 indications (58.7%) were approved without clear statistical evidence for OS improvements and seven indications (9.3%) were approved without statistical evidence for improvements on any endpoint. Strength of statistical evidence was lower for accelerated approval compared to non-accelerated approval across all three endpoints. No meaningful differences were observed for line of treatment and cancer type.LimitationsThis analysis is limited to statistical evidence. We did not consider non-statistical factors (e.g., risk of bias, quality of the evidence).ConclusionBFs offer novel insights into the strength of statistical evidence underlying cancer drug approvals. Most novel cancer drugs lack strong statistical evidence that they improve OS, and a few lack statistical evidence for efficacy altogether. These cases require a transparent and clear explanation. When evidence is ambiguous, additional post-marketing trials could reduce uncertainty.
The role of international experience and cultural intelligence in the intention to work abroad (the case of geographically and politically isolated countries)
Authors: Iskhakova Marina , Kosheleva Sofia 
Published: June 2023

PurposeThis study contributes to the conversation on international career development and its antecedents. Drawing on experiential learning theory and social cognitive theory, the current comparative study investigates the extent to which students' pre-existing international experience (IE) drives their cultural intelligence (CQ) development and influences global career intention for human resource planning purposes.Design/methodology/approachThis study has a comparative nature and adopts quantitative research methodology, which includes survey measures of CQ index, IE and intention to work abroad variables. Quantitative data are collected from a sample of more than 400 business students of leading Australian and Russian universities.FindingsThis study showed that IE variables are positively related to the level of CQ facets; the authors showed that Motivational CQ is the strongest predictor for the Intention to work abroad for both countries; the authors proved that students' IE is positively related to an Intention to work abroad for both countries and is partially mediated by CQ for Australia; and study showed that geographical isolation facilitates higher intention to work abroad than political isolation.Practical implicationsDeeper understanding of students' international career intentions and its antecedents will allow practitioners to provide better preparation for local/global careers and will allow students to make more informed and decisions. Companies would benefit from the ability to predict applicants' intention to work abroad. Stronger awareness of own preferences and available trajectories will allow students to select the best fit for them.Originality/valueThis study extends the conversation on international career development and its antecedents in the students' domain by strengthening measurements of IE and advancing the understanding of relationship between previous IE and individual facets of CQ. An empirical data from isolated locations – Russia (politically) and Australia (geographically) – bring a new timely contribution about a role of the isolation in shaping international career intentions.
Interlocking directorates and family firm performance: an emerging market’s perspective
Authors: Watkins-Fassler Karen , Rodríguez-Ariza Lázaro , Fernández-Pérez Virginia , Briano-Turrent Guadalupe del Carmen 
Published: June 2023

PurposeThis study analyses interlocking directorates from the perspective of an emerging market, Mexico, where formal institutions are weak, and family firms with high ownership concentration dominate. It responds to recent calls in the literature on interlocks, which urge the differentiation between family and non-family businesses and to complete more research on emerging economies.Design/methodology/approachA database was constructed for 89 non-financial companies (52 family-owned) listed on the Mexican Stock Exchange (BMV) from 2001 to 2014. This period includes normal times and an episode of financial crisis (2009–2010). To test the hypotheses, a dynamic panel model (in two stages) is used, applying GMM.FindingsIn normal times, the advantages of Board Chairman (COB) interlocks for the performance of publicly traded Mexican family firms are obtained regardless of the weak formal institutional environment. By contrast, during financial crisis, interlocking family COBs are more likely to jointly expropriate minority shareholders with actions that further their family objectives, which mitigates the positive effect of interlocks on performance. These findings contrast with the insignificant effects of COB interlocks found for non-family corporates.Originality/valueA new framework is proposed which, through agency theory, finds points of concordance among resource dependence and class hegemony theories, to understand the effect of interlocking directorates on the performance of family firms operating in Mexico. The results of the empirical exercise for family companies listed on BMV during normal and financial crisis periods suggest its applicability.
Environmental sustainability through aggregate demand behavior – Does knowledge economy have global responsibility?
Authors: Iqbal Mubasher , Kalim Rukhsana , Ul-Durar Shajara , Varma Arup 
Published: June 2023

Purpose
This study aims to consider environmental sustainability, a global challenge under the preview of sustainable development goals, highlighting the significance of knowledge economy in attaining sustainable aggregate demand behavior globally. For this purpose, 155 countries that have data available from 1995 to 2021 were selected. The purpose of selecting these countries is to test the global responsibility of the knowledge economy to attain environmental sustainability.

Design/methodology/approach
Results are estimated with the help of panel quantile regression. The empirical existence of aggregate demand-based environmental Kuznets curve (EKC) was tested using non-linear tests. Moreover, principal component analysis has been incorporated to construct the knowledge economy index.

Findings
U-shaped aggregate demand-based EKC at global level is validated. However, environmental deterioration increases with an additional escalation after US$497.945m in aggregate demand. As a determinant, the knowledge economy is reducing CO2 emissions. The knowledge economy has played a significant role in global responsibility, shifting the EKC downward and extending the CO2 reduction phase for every selected country. Further, urbanization, energy intensity, financial development and trade openness significantly deteriorate the environmental quality.

Originality/value
This study contains the empirical existence of aggregate demand-based EKC. The role of the knowledge economy is examined through an index which is calculated by using four pillars of the knowledge economy (technology, innovations, education and institutions). This study is based on a combined panel of all the countries for which the data was available.
Religiosity and purchase intention: an Islamic apparel brand personality perspective
Authors: Zaki Rana M. , Elseidi Reham I. 
Published: June 2023

Purpose
The aim of this research is to explore how religiosity (RG) could influence the Islamic apparel brand personality (IABP) dimensions, and to determine the degree to which IABP, attitude (ATT), subjective norms (SN) and purchase intention (PI) are influenced by RG. In addition, this research attempts to investigate the significant relationship between IABP and the components of the theory of planned behavior in the apparel industry in Egypt.

Design/methodology/approach
This research adopts a quantitative research method to provide insights relating to relationships between variables. The research data were collected through a conducted survey of Muslim females in Egypt. A convenience nonprobability sampling technique for data collection was used. To achieve the research purposes, confirmatory factor analyses, reliability and validity tests and structural equation modeling were adopted.

Findings
The research results show that RG has a positive significant relationship with ATT, SN and PI of Islamic apparel. Moreover, it was that only ATT has a positive significant influence over the PI of Islamic apparel unlike SN and Perceived behavioral control (PBC). Results also found that there is a positive relationship between IABP with ATT and SN. However, the relationship between RG and IABP was not statistically supported.

Practical implications
The research provides practical implications for brand managers, designers and producers in the Islamic apparel sector on how to increase PIs by extending IABP as well as for Egyptian policymakers. The practical implications include the possible approaches that stakeholders of Islamic apparel brands need to address while promoting, and this will influence marketing strategies in general and branding specifically.

Originality/value
This study extends our understanding of consumers’ Islamic apparel purchasing intentions using TPB to determine its rationale. Unlike other studies, this study operated RG and IABP to assess their influence on Islamic apparel PI in Egypt.
Do jihadist terrorist attacks cause changes in institutional trust? A multi‐site natural experiment
Authors: NÄGEL CHRISTOF , NIVETTE AMY , CZYMARA CHRISTIAN 
Published: June 2023

AbstractResults from previous research suggest that terrorist attacks lead to relatively short‐term increases in trust in institutions. The explanation for this increase is known as the ‘rally effect’, whereby individuals respond to crises and threats with more positive support for political leaders and institutions. Even though the number of related natural experiments with survey data is increasing, these studies merely represent case studies of single incidents with limited external validity. To advance quasi‐experimental research on the effects of terrorist attacks on institutional trust, we propose a new methodological approach by assessing all jihadist terrorist attacks resulting in at least one civilian death in a European country that take place during the fieldwork of the European Social Survey and combining the results of eight unique natural experiments in five different countries using meta‐analytic and meta‐regression techniques. The results of this ‘multi‐site natural experiment’ indicate that support for the rally‐hypothesis is mixed at best. While some attacks appear to significantly increase at least some measures of institutional trust (e.g., The Netherlands 2004, France 2015, Israel 2012), others seem to have no effect at all (e.g., Germany 2015, France 2018), or even substantially decrease trust in domestic political institutions (Russia 2012). Summary effects from multilevel meta‐analyses are non‐significant for any institutional trust outcome. These results are robust to a large number of robustness tests and alternative specifications. In comparison with previous research, it appears that a lot of the European evidence for the rally‐hypothesis was based on ‘outlier’ case studies like the Charlie Hebdo attack in France, 2015. Accordingly, our results cast doubt on the unrestricted generalisability of rally effects after terrorist attacks to different geographic, political, social or historical contexts.
Knockdown of circADAM9 inhibits cell progression and glycolysis by targeting the miR‐1236‐3p/FGF7 axis in breast cancer
Authors: Huang Bo , Zhang Yichao , Sun Peng , Lin Jianshan , Wang Cunchuan 
Published: June 2023

AbstractBackgroundCircular RNAs (circRNAs) are closely associated with the development of breast cancer (BC). In this study, we aimed to clarify how differentially expressed circRNAs affect the development of BC.MethodsQuantitative real‐time polymerase chain reaction (qRT‐PCR) was used to detect the expression of circADAM9, miR‐1236‐3p and fibroblast growth factor 7 (FGF7). Colony formation, 5‐ethynyl‐2′‐deoxyuridine (EdU), wound healing, transwell, and flow cytometry were used to assess cell proliferation, migration, invasion, and apoptosis. Glucose consumption, lactic acid production and ATP levels were assessed using glycolysis metabolism analysis. Dual‐luciferase reporter assay and RNA immunoprecipitation (RIP) assay were carried out to verify the relationship between miR‐1236‐3p and circADAM9 or FGF7. The roles of cirADAM9 on tumor growth were analyzed using a xenograft tumor model. Ki‐67 and FGF7 expression was measured via immunohistochemistry (IHC) assay. Apoptosis‐related proteins and exosome markers were detected by western blot.ResultsCircADAM9 was highly expressed in BC cells, and circADAM9 silencing inhibited BC cell proliferation, migration, invasion, and glycolysis, and promoted cell apoptosis. Furthermore, miR‐1236‐3p inhibition could overturn circADAM9 knockdown‐mediated BC inhibition. Moreover, the negative influences of miR‐1236‐3p overexpression on BC progression were restrained via FGF7 overexpression. CircADAM9 silence also inhibited BC tumor growth in vivo.ConclusionCircADAM9 promoted BC development partly by the miR‐1236‐3p/FGF7 axis, highlighting a potential prognostic biomarker and therapeutic target for BC patients.
CATE: A fast and scalable CUDA implementation to conduct highly parallelized evolutionary tests on large scale genomic data
Authors: Perera Deshan , Reisenhofer Elsa , Hussein Said , Higgins Eve , Huber Christian D. , Long Quan 
Published: June 2023

Abstract

Statistical tests for molecular evolution provide quantifiable insights into the selection pressures that govern a genome's evolution. Increasing sample sizes used for analysis leads to higher statistical power. However, this requires more computational nodes or longer computational time.

CATE (CUDA Accelerated Testing of Evolution) is a computational solution to this problem comprised of two main innovations. The first is a file organization system coupled with a novel search algorithm and the second is a large‐scale parallelization of algorithms using both graphical processing unit (GPU) and central processing unit. CATE is capable of conducting evolutionary tests such as Tajima's D, Fu and Li's, and Fay and Wu's test statistics, McDonald–Kreitman Neutrality Index, Fixation Index and Extended Haplotype Homozygosity.

CATE is magnitudes faster than standard tools with benchmarks estimating it being on average over 180 times faster. For instance, CATE processes all 54,849 human genes for all 22 autosomal chromosomes across the five super populations present in the 1000 Genomes Project in less than 30 min while counterpart software took 3.62 days.

This proven framework has the potential to be adapted for GPU‐accelerated large‐scale parallel analyses of many evolutionary and genomic analyses.
The relationship between ICU survivorship, comorbidity and educational level in quality of life after intensive care
Authors: Malmgren Johan , Johannesson Elias , Waldenström Ann‐Charlotte , Rylander Christian , Lundin Stefan 
Published: June 2023

AbstractBackgroundICU survivors have lower quality of life (QoL) compared to a non‐ICU‐treated population. The reason for this is not fully understood, but differences in baseline characteristics may be an important factor. This study evaluates the roles of comorbidity and educational level as possible explanatory factors for differences in QoL in ICU survivors compared to a non‐ICU‐treated population.MethodsUsing a provisional questionnaire with 218 questions in 13 domains on QoL after intensive care, we compared the responses from 395 adult ICU survivors and 195 non‐ICU‐treated controls. An initial bivariable linear correlation analysis compared the responses from the two groups. Two secondary multivariable regression analyses tested for effect modification by comorbidity and educational level, respectively, on the effect on QoL from belonging to the ICU survivor group compared to the control group.ResultsThere was a significant difference in QoL between the two groups in 170 of 218 (78%) questions. In the multivariable analyses, associations between group belonging and QoL remained in 139 questions. In 59 of these, both belonging to the ICU survivor group and comorbidity was associated to QoL, in parallel to each other. Comorbidity moderated the association between group belonging and QoL in six questions, with the highest proportion of questions within the domains of cognition and urinary functions, and the lowest proportion within appetite and alcohol, physical health, and fatigue. In 26 questions, both belonging to the ICU survivor group and educational level were associated to QoL, in parallel to each other. Educational level moderated the association between group belonging and QoL in 34 questions, with the highest proportion of questions within the domains of urinary functions, ADL and physical health, and the lowest proportion within the domains of cognition, appetite and alcohol, pain, sensory functions, and fatigue.ConclusionsLower QoL seen in ICU survivors compared to non‐ICU‐treated controls, as measured by our provisional questionnaire, cannot be explained only by a higher burden of comorbidity, and rarely by only educational level. In issues where comorbidity or educational level was associated to QoL, it often was so in parallel to an association from belonging to the ICU survivor group. Comparing QoL in ICU survivors to that of a non‐ICU‐treated population may be adequate despite differences in baseline characteristics.
Monozygotic twins with identical premature timing of acne onset: A Case report
Authors: Tan Amanda S. H. , Chia Shi Yun , Lim Jiin Ying , Chan Daniel 
Published: June 2023

AbstractAcne vulgaris, a common dermatological condition that affects most adolescents and young adults, can indicate an underlying pathology if present prematurely in mid‐childhood. Premature acne can be caused by premature adrenarche secondary to non‐classical congenital adrenal hyperplasia (NC‐CAH), a condition arising from 21‐hydroxylase deficiency. This report describes a pair of monozygotic twin brothers with identical premature onset of acne, who were found to have an identical homozygous mutation in the promoter region of the CYP21A2 gene. While it is widely known that NCCAH is associated with genetic changes, the drive behind onset of adrenarche are widely unknown. As such, this report provokes thoughts on whether adrenarche could be influenced by adrenal genetic polymorphisms.