bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential quorum-sensing inhibitor of
Hafnia alvei
H4—theaflavin-3,3´-digallate analyzed by virtual screening and molecular simulation
Authors: Li Xue , Yan Congyang , Wang Yanan , Zhang Gongliang , Bi Jingran , Hao Hongshun , Hou Hongman 
Published: September 2023

ABSTRACT

Hafnia
species can cause food spoilage via the quorum-sensing (QS) system. Thus, strategies that target QS in these bacteria might be a good approach to safeguard the quality of processed food. In this study, the amino acid sequence of the LasI

Ha

protein, a key QS regulator from
Hafnia alvei
H4, was used to construct its 3D structure for the virtual screening of potential QS inhibitors (QSIs) from the Bioactive Compound database. Four potential QSIs were obtained, and these were all theaflavins (TFs). Among them, theaflavin-3,3´-digallate (TF3) was found to outperform the others, displaying a higher docking score according to molecular docking analysis, and required only a sub-minimal inhibitory concentration (31.25 mM) to cause a significant decrease in the production of the autoinducer N-acyl homoserine lactone in
H. alvei
H4 and up to 60.5% inhibition of its motility. Furthermore, molecular simulation results indicated that TF3 could stably bind to a cavity within LasI

Ha

to form stable hydrogen bonds and hydrophobic interactions with various key residues of the protein to exert the inhibitory effect. Thus, TF3 may be considered a potential compound to protect against food spoilage caused by
H. alvei
H4 via the quorum quenching.

IMPORTANCE

Hafnia alvei
, the main strain studied in this paper, is often isolated from spoiled foods, especially refrigerated protein-based raw foods, and is generally considered to be a spoilage bacterium whose spoilage-causing properties may be closely related to its own very strong population-sensing activity, so the strategy of quorum quenching against
H. alvei
H4 may be a good way to guarantee the quality of processed foods. Given the current global requirements for food safety and quality, coupled with negative consumer perceptions of the excessive inclusion of synthetic chemicals in food products, the use of natural compounds as QSIs in the storage of aquatic food products would seem more attractive.
In vitro
activities of contezolid (MRX-I) against drug-sensitive and drug-resistant
Mycobacterium tuberculosis
Authors: An Huiru , Sun Wenna , Liu Xiao , Wang Tianhao , Qiao Juan , Liang Jianqin 
Published: September 2023

ABSTRACT

A novel oxazolidinone for the treatment of
Mycobacterium tuberculosis
has been developed, but the activity of contezolid (MRX-I) still needs to be clarified. In this study, we isolated
Mycobacterium tuberculosis
from 48 clinical patients with pulmonary tuberculosis. Roche drug susceptibility tests identified drug-sensitive and 39 drug-resistant
M. tuberculosis
isolates. Drug susceptibility assays indicated that MRX-I exhibited anti-
Mycobacterium tuberculosis
activity against both drug-sensitive and drug-resistant isolates, with an advantage against drug-resistant isolates. The results also showed that the anti-
Mycobacterium tuberculosis
activity was comparable to that of linezolid.

IMPORTANCE

Currently,
Mycobacterium tuberculosis
has exhibited increased drug resistance, leading to ineffective drug treatment in many patients with tuberculosis. Among the anti-
Mycobacterium tuberculosis
drugs, oxazolidinones have been gradually developed. Contezolid (MRX-I) has been newly developed in China with advantages versus the first oxazolidinone antibiotic approved by the Food and Drug Administration for clinical use, but the anti-
M. tuberculosis
activity needs to be further clarified. In this study, in vitro activities of MRX-I against
M. tuberculosis
were tested. The drug susceptibility assays indicated that MRX-I exhibited anti-
M. tuberculosis
activity comparable to that of linezolid, with an advantage against drug-resistant isolates.
Ongoing fragmentation of the subducting Cocos slab, Central America
Authors: Xue Tu , Peng Diandian , Liu Kelly H. , Obrist-Farner Jonathan , Locmelis Marek , Gao Stephen S. , Liu Lijun 
Published: September 2023

Abstract
Fundamental to plate tectonics is the subduction of cold and mechanically strong oceanic plates. While the subducted plates are conventionally regarded to be impermeable to mantle flow and separate the mantle wedge and the subslab region, isolated openings have been proposed. By combining new shear wave splitting measurements with results from geodynamic modeling and recent seismic tomography and geochemical observations, we show that the upper ~200 km of the Cocos slab in northern Central America is intensively fractured. The slab there is strong enough to produce typical arc volcanoes and Benioff Zone earthquakes but allows mantle flow to traverse from the subslab region to the mantle wedge. Upwelling of hot subslab mantle flow through the slab provides a viable explanation for the behind-the-volcanic-front volcanoes that are geochemically distinct from typical arc volcanoes, and for the puzzling high heat flow, high elevation, and low Bouguer gravity anomalies observed in northern Central America.
Correlation of Short-Period Wave Disturbances of the Peak Electron Density of the F2 Layer and the Total Electron Content in the Ionosphere
Authors: Perevalova N. P. , Ratovsky K. G. , Zherebtsov G. A. , Yasyukevich A. S. 
Published: September 2023

AbstractCorrelation analysis of short-period (periods less than 24 h) wave disturbances of the maximum electron density in the F2 layer (NmF2) and the total electron content (TEC) was analyzed based on the data of the vertical ionosonde sounding and receiver of the Global Navigation Satellite Systems operating in Irkutsk. The measurement data in the period 2003–2020, which included almost two solar cycles, have been processed. It was found that the most likely case is a high positive correlation of short-period NmF2 and TEC disturbances. The number of cases of strong positive correlation increases (from 53 to 66%), and the number of cases of positive and negative weak correlation decreases (from 46 to 33%) as the solar and geomagnetic activity increases. During strong geomagnetic storms, the highest correlation (up toR= 0.89) is observed in the main phase of the storm and then decreases in the recovery phase to negativeRvalues. Possible physical mechanisms of the results are considered.
Circulating metabolites improve the prediction of renal impairment in patients with type 2 diabetes
Authors: Trischitta Vincenzo , Mastroianno Mario , Scarale Maria Giovanna , Prehn Cornelia , Salvemini Lucia , Fontana Andrea , Adamski Jerzy , Schena Francesco Paolo , Cosmo Salvatore De , Copetti Massimiliano , Menzaghi Claudia 
Published: September 2023

IntroductionLow glomerular filtration rate (GFR) is a leading cause of reduced lifespan in type 2 diabetes. Unravelling biomarkers capable to identify high-risk patients can help tackle this burden. We investigated the association between 188 serum metabolites and kidney function in type 2 diabetes and then whether the associated metabolites improve two established clinical models for predicting GFR decline in these patients.Research design and methodsTwo cohorts comprising 849 individuals with type 2 diabetes (discovery and validation samples) and a follow-up study of 575 patients with estimated GFR (eGFR) decline were analyzed.ResultsTen metabolites were independently associated with low eGFR in the discovery sample, with nine of them being confirmed also in the validation sample (ORs range 1.3–2.4 per 1SD, p values range 1.9×10−2–2.5×10−9). Of these, five metabolites were also associated with eGFR decline (ie, tiglylcarnitine, decadienylcarnitine, total dimethylarginine, decenoylcarnitine and kynurenine) (β range −0.11 to −0.19, p values range 4.8×10−2to 3.0×10−3). Indeed, tiglylcarnitine and kynurenine, which captured all the information of the other three markers, improved discrimination and reclassification (all p<0.01) of two clinical prediction models of GFR decline in people with diabetes.ConclusionsFurther studies are needed to validate our findings in larger cohorts of different clinical, environmental and genetic background.
Omalizumab for Chinese patients with moderate-to-severe allergic asthma in real-world clinical setting: a prospective, observational study
Authors: Wang Wen , Li Jieying , Zou Congying , Zhao Lifang , Zhu Ya , Guo Yue , Wang Feiran 
Published: September 2023

BackgroundWe aimed to investigate the effectiveness of omalizumab, a monoclonal anti-immunoglobulin E antibody, in Chinese patients with moderate-to-severe allergic asthma in real-world clinical practice.MethodsThis single-centre, prospective, observational study included Chinese patients aged 14–75 years with moderate-to-severe allergic asthma according to the Global Initiative for Asthma criteria. Omalizumab was administered subcutaneously, and the investigator collected real-world data on exacerbations, steroid exposure, pulmonary function and laboratory assessments at weeks 16, 24, 52, 104 and 156 after treatment initiation. The primary outcome was reduced exacerbations, measured as the proportion of patients with exacerbations in the year following omalizumab initiation. Bowker’s test for paired proportions was performed to compare exacerbation rates before and after treatment initiation. A generalised linear mixed model was used to compare the number of exacerbations.ResultsThe mean treatment duration was 46.6 weeks for the full analysis set (n=398). The proportion of patients with exacerbations in the year before and after omalizumab initiation was 80.4% (181/225) and 18.7% (42/225) (difference: −61.8%, 95% CI −68.5 to –54.0, p<0.0001), respectively. At week 52, 67.4% of patients discontinued oral corticosteroids, and 19.5% reduced inhaled corticosteroids. The Asthma Control Test scores increased by 4.6 at week 52 from baseline (p<0.001). Forced expiratory volume in 1 s increased by 11.2% and 9.0% at weeks 24 and 52, respectively, from baseline (p<0.01). Injection site reactions (5.2%) were the most frequently reported adverse event.ConclusionsIn real-world clinical practice, omalizumab treatment remarkably reduced exacerbations in Chinese patients with moderate-to-severe asthma. Omalizumab reduced the use of oral corticosteroids and improved asthma control and pulmonary function.
Health problems occurring in national-level female soccer players are different between leagues and throughout the season: a 6-month prospective cohort study
Authors: Dettwiler Anna , Wieloch Nora , Fröhlich Stefan , Imhoff Florian , Scherr Johannes , Spörri Jörg 
Published: September 2023

ObjectivesThis study investigated the prevalence and severity of health problems in national-level female soccer players with respect to league and seasonality.MethodsIn a prospective cohort study, 46 female soccer players aged 22.8±3.9 years playing in the three highest leagues in Switzerland were surveyed biweekly using the Oslo Sports Trauma Research Centre health problem (OSTRC-H) questionnaire. All definitions and measures followed the OSTRC-H-specific recommendations. The 6-month observation period included parts of the off-season and one half of the match season.ResultsThe average 2-weekly health problem prevalence was 37.3% (illnesses: 8.8%; sudden onset injuries—both acute and repetitive mechanisms: 19.7%; repetitive gradual onset injuries: 12.4%) and 25.1% for substantial problems as defined in the OSTRC-H context (7.3%; 12.0% and 7.3%, respectively). The absolute injury rates amounted to 148 injuries per 100 players per half season, of which 96 injuries per 100 players per half season were substantial. Female players in the 2nd and 3rd highest national leagues showed more gradual onset injuries (p<0.001) and fewer illnesses than those in the top league (p<0.05). At the same time, there were no league-specific differences in sudden onset injuries. Such injuries had a higher cumulative severity score than gradual onset injuries. Among sudden onset injuries, the ankle was the most affected body part, while the thigh was affected by for gradual onset injuries. The average 2-weekly health problem prevalence values steadily increased during the match season.ConclusionAmong national-level female soccer players, the risk of health problems is relatively high and differs between leagues and across seasons.
Disruption of CerS6-mediated sphingolipid metabolism by FTO deficiency aggravates ulcerative colitis
Authors: Ma Yanru , Zhang Xinyu , Xuan Baoqin , Li Danjie , Yin Nan , Ning Lijun , Zhou Yi-Lu , Yan Yuqing , Tong Tianying , Zhu Xiaoqiang , Huang Xiaowen , Hu Muni , Wang Zhenhua , Cui Zhe , Li Huabin , Wang Jiqiu , Fang Jing-Yuan , Liu Ruixin , Chen Haoyan , Hong Jie 
Published: September 2023

Background and aimsDeregulation of RNA N6-methyladenosine (m6A) modification in intestinal epithelial cells (IECs) influences intestinal immune cells and leads to intestinal inflammation. We studied the function of fat mass-and obesity-associated protein (FTO), one of the m6A demethylases, in patients with ulcerative colitis (UC).MethodsWe analysed colon tissues of Ftoflox/flox; Villin-cre mice and their Ftoflox/floxlittermates with dextran sulfate sodium (DSS) using real-time PCR and 16s rRNA sequencing. RNA and methylated RNA immunoprecipitation sequencing were used to analyse immunocytes and IECs. Macrophages were treated with conditioned medium of FTO-knockdown MODE-K cells or sphingosine-1-phosphate (S1P) and analysed for gene expression. Liquid chromatograph mass spectrometry identified C16-ceramide.ResultsFTO downregulation was identified in our in-house cohort and external cohorts of UC patients. Dysbiosis of gut microbiota, increased infiltration of proinflammatory macrophages, and enhanced differentiation of Th17 cells were observed in Ftoflox/flox;Villin-cre mice under DSS treatment. FTO deficiency resulted in an increase in m6A modification and a decrease in mRNA stability of CerS6, the gene encoding ceramide synthetase, leading to the downregulation of CerS6 and the accumulation of S1P in IECs. Subsequentially, the secretion of S1P by IECs triggered proinflammatory macrophages to secrete serum amyloid A protein 1/3, ultimately inducing Th17 cell differentiation. In addition, through bioinformatic analysis and experimental validation, we identified UC patients with lower FTO expression might respond better to vedolizumab treatment.ConclusionsFTO downregulation promoted UC by decreasing CerS6 expression, leading to increased S1P accumulation in IECs and aggravating colitis via m6A-dependent mechanisms. Lower FTO expression in UC patients may enhance their response to vedolizumab treatment.
Comparative analysis of long term effectiveness of Neuroform Atlas stent versus low profile visualized intraluminal stent/Woven EndoBridge devices in treatment of wide necked intracranial aneurysms
Authors: Salem Mohamed M , Jankowitz Brian T , Burkhardt Jan-Karl , Price Lori Lyn , Zaidat Osama O 
Published: September 2023

BackgroundWe compared the outcomes of wide necked aneurysms (WNA) treated with the Neuroform Atlas with those treated with the low profile visualized intraluminal stent (LVIS) or the Woven EndoBridge (WEB).MethodsObjective, prospectively collected, core laboratory adjudicated data from published trials for the Neuroform Atlas, LVIS, and WEB devices were reviewed. ATLAS (Safety and Effectiveness of the Treatment of Wide Neck, Saccular Intracranial Aneurysms With the Neuroform Atlas Stent System) study patients were included if they met other studies’ inclusion criteria. Outcomes included (1) primary effectiveness (complete aneurysmal occlusion without retreatment/>50% parent vessel stenosis), (2) primary safety, (3) complete aneurysmal occlusion, and (4) retreatment rates (outcomes evaluated at the 12 month follow-up). Matching adjusted indirect comparison analysis was used to compare outcomes.ResultsAnalytical samples included 141 ATLAS subjects meeting WEB-IT (Woven EndoBridge Intrasaccular Therapy Study) criteria (ATLAS/WEB-IT) and 241 meeting LVIS (Pivotal Study of the Low Profile Visualized Intraluminal Support) criteria (ATLAS/LVIS). ATLAS/WEB-IT exhibited significantly higher rates of primary effectiveness and complete occlusion versus WEB (86.6% vs 53.9 %, P<0.0001, and 90.3% vs 53.9%, P<0.0001, respectively). For LVIS, there was no significant differences in primary effectiveness rates between ATLAS and LVIS (84.2% vs 77.7%, respectively, P=0.12). However, ATLAS/LVIS had a significantly higher proportion of patients achieving complete occlusion than LVIS (88.1 vs 79.1, P=0.03). Retreatment rates and primary safety outcomes were not significantly different (P>0.05) for the Atlas versus other devices except for a lower retreatment rate for ATLAS/WEB-IT versus WEB-IT (2.4% vs 9.8%, P=0.01).ConclusionThe Neuroform Atlas provided higher occlusion rates and similar retreatment rates in comparable datasets compared with LVIS and WEB devices when treating WNA.
Derivation and validation of a predictive scale to expedite endovascular intervention for acute stroke patients with an intervenable vessel occlusion
Authors: Ren Zeguang , Wangqin Runqi , Demiraj Francis , Li Weizhe , Mokin Maxim , Wang Anxin , Miao Zhongrong , Wang Yongjun , Burgin W Scott 
Published: September 2023

BackgroundEarly endovascular intervention team mobilization may reduce reperfusion times and improve clinical outcomes for patients with acute ischemic stroke (AIS) with a possible intervenable vessel occlusion (IVO). In an emergency department or mobile stroke unit, incorporating rapidly available non-contrast CT (NCCT) information with examination findings may improve the accuracy of arterial occlusion prediction scales. For this purpose, we developed a rapid and straightforward IVO predictive instrument—the T3AM2PA1scale.MethodsThe T3AM2PA1scale was retrospectively derived from our ‘Get with the Guidelines’ database. We included all patients with acute stroke alert between January 2017 and August 2018 with a National Institutes of Health Stroke Scale (NIHSS) score between 5 and 25 inclusive. Different pre-intervention variables were collected, including itemized NIHSS and NCCT information. The T3AM2PA1scale was also compared with other commonly used scales and was validated in a separate sequential retrospective cohort of patients with a full range of NIHSS scores.Results574 eligible patients from 2115 acute stroke alerts were identified. The scale was established with five items (CT hyperdense sign, parenchymal hypodensity, lateralizing hemiparesis, gaze deviation, and language disturbance), with a total score of 9. To minimize unnecessary angiography, a cut-off of ≥5 for IVO detection yielded a sensitivity of 52%, a specificity of 90%, and a positive predictive value of 76%.ConclusionsThe T3AM2PA1scale accurately predicts the presence of clinical IVO in patients with AIS. Adopting the T3AM2PA1scale could reduce revascularization times, improve treatment outcomes, and potentially reduce disability.
Identification of the first signs or symptoms in different spondyloarthritis subtypes and their association with HLA-B27: data from REGISPONSER and RESPONDIA registries
Authors: Puche-Larrubia María Ángeles , Ladehesa-Pineda Lourdes , Vázquez-Mellado Janitzia , Escudero-Contreras Alejandro , Gratacós Jordi , Juanola Xavier , Collantes-Estévez Eduardo , Font-Ugalde Pilar , López-Medina Clementina 
Published: September 2023

ObjectiveTo describe and analyse the initial symptoms attributable to patients with spondyloarthritis (SpA) and their association with HLA-B27 status.MethodsThis was an observational, cross-sectional and multicentre study with patients who fulfilled the European Spondyloarthropathy Study Group criteria for SpA from the Registry of Spondyloarthritis of Spanish Rheumatology (REGISPONSER) and Ibero-American Registry of Spondyloarthropathies (RESPONDIA) united registries. Differences in the first sign(s) or symptom(s) were compared across diagnoses and between HLA-B27 status. The diagnostic delay between patients who start the disease with musculoskeletal manifestations (MMs) and extra-MMs (EMMs) was compared.ResultsA total of 4067 patients were included (2208 from REGISPONSER and 1859 from RESPONDIA) (ankylosing spondylitis (AS): 68.3%, psoriatic arthritis (PsA): 19.9%, undifferentiated SpA: 11.8%). Overall, 3624 (89.1%) patients initiated the disease with MMs and 443 (10.9%) with EMMs. Low back pain (61.7%) and lower-limb arthritis (38.5%) were the most frequent initial symptoms. In AS patients, the absence of HLA-B27 seems to be related to an increase in the probability of starting the disease with cervical pain and peripheral manifestations. In PsA, the onset of arthritis and psoriasis was more prevalent in HLA-B27-negative patients, while initiation with axial manifestations was more predominant in HLA-B27-positive patients. The diagnostic delay was longer in patients with initial MMs than in those with EMMs (7.2 (34.8) vs 4.5 (7.6) years, respectively).ConclusionIn this SpA population, MMs were the most prevalent initial symptoms, with differences across diagnoses and depending on the presence of the HLA-B27 antigen.
Novel electromagnetic navigation bronchoscopy system for the diagnosis of peripheral pulmonary nodules: a prospective, multicentre study
Authors: Li Ying , Chen Wei , Xie Fangfang , Huang Rui , Liu Xiang , Xiao Yang , Cao Liming , Hu Yi , Ke Mingyao , Wu Shiman , Sun Jiayuan 
Published: September 2023

BackgroundTraditional electromagnetic navigation bronchoscopy (ENB) is a real-time image-guided system and used with thick bronchoscopes for the diagnosis of peripheral pulmonary nodules (PPNs). A novel ENB that could be used with thin bronchoscopes was developed. This study aimed to evaluate the diagnostic yield and the experience of using this ENB system in a real clinical scenario.MethodsThis multicentre study enrolled consecutive patients with PPNs adopting ENB from March 2019 to August 2021. ENB was performed with different bronchoscopes, ancillary techniques and sampling instruments according to the characteristics of the nodule and the judgement of the operator. The primary endpoint was the diagnostic yield. The secondary endpoints included the diagnostic yield of subgroups, procedural details and complication rate.ResultsIn total, 479 patients with 479 nodules were enrolled in this study. The median lesion size was 20.9 (IQR, 15.9–25.9) mm. The overall diagnostic yield was 74.9% (359/479). A thin bronchoscope was used in 96.2% (461/479) nodules. ENB in combination with radial endobronchial ultrasound (rEBUS), a guide sheath (GS) and a thin bronchoscope was the most widely used guided method, producing a diagnostic yield of 74.1% (254/343). The median total procedural time was 1325.0 (IQR, 1014.0–1676.0) s. No severe complications occurred.ConclusionThis novel ENB system can be used in combination with different bronchoscopes, ancillary techniques and sampling instruments with a high diagnostic yield and safety profile for the diagnosis of PPNs, of which the combination of thin bronchoscope, rEBUS and GS was the most common method in clinical practice.Trial registration numberNCT03716284.
Efficient negative-weight elimination in large high-multiplicity Monte Carlo event samples
Authors: Andersen Jeppe R. , Maier Andreas , Maître Daniel 
Published: September 2023

AbstractWe demonstrate that cell resampling can eliminate the bulk of negative event weights in large event samples of high multiplicity processes without discernible loss of accuracy in the predicted observables. The application of cell resampling to much larger data sets and higher multiplicity processes such as vector boson production with up to five jets has been made possible by improvements in the method paired with drastic enhancement of the computational efficiency of the implementation.