bluuebunny/crossref_metadata_2025_split huggingface dataset:
International beliefs and head positioning practices in patients with spontaneous hyperacute intracerebral hemorrhage
Authors: Dusenbury Wendy , Malkoff Marc D. , Schellinger Peter D. , Köhrmann Martin , Arthur Adam S. , Elijovich Lucas , Alexandrov Andrei V. , Tsivgoulis Georgios , Alexandrov Anne W. 
Published: March 2023

Background: Prior to the conduct of the Head Position in Stroke Trial (HeadPoST), an international survey ( n = 128) revealed equipoise for selection of head position in acute ischemic stroke. Objectives: We aimed to determine whether equipoise exists for head position in spontaneous hyperacute intracerebral hemorrhage (ICH) patients following HeadPoST. Design: This is an international, web-distributed survey focused on head positioning in hyperacute ICH patients. Methods: A survey was constructed to examine clinicians’ beliefs and practices associated with head positioning of hyperacute ICH patients. Survey items were developed with content experts, piloted, and then refined before distributing through stroke listservs, social media, and purposive snowball sampling. Data were analyzed using descriptive statistics and χ2 test. Results: We received 181 responses representing 13 countries on four continents: 38% advanced practice providers, 32% bedside nurses, and 30% physicians; overall, participants had median 7 [interquartile range (IQR) = 3–12] years stroke experience with a median of 100 (IQR = 37.5–200) ICH admissions managed annually. Participants disagreed that HeadPoST provided ‘definitive evidence’ for head position in ICH and agreed that their ‘written admission orders include 30-degree head positioning’, with 54% citing hospital policies for this head position in hyperacute ICH. Participants were unsure whether head positioning alone could influence ICH longitudinal outcomes. Use of serial proximal clinical and technology measures during the head positioning intervention were identified by 82% as the most appropriate endpoints for future ICH head positioning trials. Conclusion: Interdisciplinary providers remain unconvinced by HeadPoST results that head position does not matter in hyperacute ICH. Future trials examining the proximal effects of head positioning on clinical stability in hyperacute ICH are warranted.
The Effects of Resveratrol Targeting MicroRNA-4325P/PDGF-B to Regulate Tumor Angiogenesis in Osteosarcoma Microenvironment
Authors: Liu Shouzheng , Jiang Hongyan , Liu Shoushan , Li Yecheng 
Published: March 2023

Objectives Here we sought to determine the specific mechanism by which resveratrol targets microRNA-4325p and PDGFB in an osteosarcoma (OS) cell line and investigate its effect on the OS cell line. Methods As a result of the study related to the effect of resveratrol on the conditioned media of microRNA-4325p mimic transfected MG-63 and HOS (a fibroblast and epithelial-like cell line) cells, we determined that resveratrol decreased survival, and growth, migration, and aortic ring formation of endothelial cells. A combination of miRNA mimics and HOS and MG-63 cell lines were transfected with inhibitors to explore the mechanisms behind angiogenesis formation induced by resveratrol. Results We found that resveratrol improved the activity of microRNA-4325p mimic transfection in OS, thus promoting angiogenesis, as demonstrated by angiogenic parameters and gene expression in OS. Following these results, it is evident that resveratrol can modulate angiogenesis by regulating microRNA-4325p expression by targeting PDGFB genes in OS cells. Conclusion The results demonstrate that resveratrol promotes tumor angiogenesis of OS cells through microRNA-4325p/PDGBB-mediated pathways and provides theoretical and experimental support for the discovery of new bioactive phytochemicals that are effective therapeutic agents in the prevention and treatment of OS diseases.
Blood donation practice and its predictors among undergraduate college students in Harari Regional State, Eastern Ethiopia
Authors: Idris Elias , Yadeta Elias , Debella Adera , Tamiru Dawit , Atnafe Genanaw , Arkew Mesay , Teklemariam Zelalem 
Published: March 2023

Objectives: The main objective of this study was to assess blood donation practice and its associated factors among undergraduate college students in Harari Region, Eastern Ethiopia. Methods: An institutional-based cross-sectional study was employed among 518 college students selected by using a simple random sampling technique. Data was collected using pretested structured self-administered questionnaire. The collected data was entered into Epi-data 3.41 and exported to Statistical Package for Social Science version 22 for analysis. Bivariate and multivariable logistic regressions were utilized to identify factors associated with blood donation practice. p-Values of 0.05 or less was used to declare statistical significance. Results: In this study, the overall blood donation practice was 35.7% (95% confidence interval: 31.6, 39.8). Students studying health sciences were more likely than non-health sciences students (53.5%) to donate blood. Having positive knowledge about blood donation (adjusted odds ratio = 4.17; 95% confidence interval: 2.50, 6.92), being male (adjusted odds ratio = 0.57; 95% confidence interval: 0.38, 0.87), being student of midwifery department (adjusted odds ratio = 2.16; 95% confidence interval: 1.07, 4.36) and nursing department (adjusted odds ratio = 2.42; 95% confidence interval: 1.18, 4.98) were significantly associated with blood donation practice. Conclusion: Practice of blood donation among college students in the study is relatively low. Knowledge about blood donation, male sex and being a nursing and midwifery student were independently associated with blood donation practice. Therefore, the Regional Health Bureau and Blood Bank in collaboration with college administrators should design and implement appropriate strategies to improve blood donation practice.
Strategy to increase vaccination coverage in diabetic patients at a public tertiary university hospital: A randomized controlled trial
Authors: Guerra Giulia Limana , Pedro Fabio Lopes , Severo Mateus Dornelles , Guerra Giorgia Limana , Ribeiro Tiango Aguiar 
Published: March 2023

Objective: To evaluate whether there is an increase in vaccination rates of patients with diabetes mellitus who received guidance to update their vaccination schedules for influenza, hepatitis B, pneumonia, and tetanus. Methodology: A randomized controlled trial was conducted between December 2018 and November 2020. The sample consisted of 139 patients from the endocrinology service outpatient clinic of Santa Maria University Hospital was randomized into an intervention group ( n = 68) and a control group ( n = 71). The intervention consisted of a phone call to update the vaccination schedule for the diseases evaluated. Results: The mean age of the subjects was 59.17 ± 12.91 years and 62.6% were female. No age differences were observed between genders and randomization groups ( p = 0.548, p = 0.791) and groups were homogeneous ( p = 0.173, p = 0.443). The intervention group showed a significant increase in vaccination rates after the intervention. For influenza, 79.4–89.7% ( p = 0.016); hepatitis B, 29.4–48.5% ( p = 0.002); tetanus, 51.5–72.1% ( p = 0.007); and pneumonia, 22.1–29.4% ( p = 0.049). No significant increase was observed in control group. Conclusion: The orientation to update the vaccination schedule through telephone contact was effective in increasing vaccination rates for influenza, hepatitis B, pneumonia, and tetanus. Trial registry: RBR-92z99d2 https://ensaiosclinicos.gov.br/rg/RBR-92z99d2
Disparities in Perceived Physical and Mental Wellness: Relationships Between Social Vulnerability, Cardiovascular Risk Factor Prevalence, and Health Behaviors Among Elderly US Residents
Authors: Jacobs Molly M. , Burch Ashley E. 
Published: March 2023

Introduction: Cardiovascular disease (CVD) is the leading cause of death for males, females, and people of most racial and ethnic groups in the United States. In addition to known epidemiological and behavioral risk factors, recent evidence suggests that circumstantial or behavioral factors may also be associated with CVD. This study evaluates the contribution of cardiovascular disease (CVD) risk factors, community vulnerabilities, and individual health behaviors to individual physical and mental wellness among Black and White, male, and female Medicare beneficiaries. Methods: This study utilized data from the Behavioral Risk Factor Surveillance System, county-level CVD risk factor prevalence, and selected items from the Social Vulnerability Index. Results: Correlations were found between unhealthy days reported by males and area social vulnerabilities and health behaviors. Prevalence of disease was correlated with mentally unhealthy days for White males. Among White females, health behaviors, disease prevalence, and social vulnerability measures were associated with unhealthy days. Among Black females, disease prevalence was highly correlated with mentally unhealthy days. Discussion: While individual-level health behaviors are highly associated with perceived physical and mental health, the self-reported health of Black respondents is also heavily correlated with local area vulnerabilities, including community poverty, group housing, and crowding.
Comparison of Migraine Characteristics in Patients With and Without Inflammatory Bowel Disease: A Retrospective Cohort Study
Authors: Sileno Sean M. , Bi Yan , Dorenkott Sean M. , Omer Mohamed , Salih Ahmed , Ghoz Hassan M. , Valery Jose R. , Harris Dana M. , Rozen Todd D. , Heckman Michael G. , White Launia J. , Stancampiano Fernando F. 
Published: March 2023

Background: Survey studies have found an increased prevalence of migraine in patients with inﬂammatory bowel disease (IBD). However, the clinical characteristics of migraines in this population are unknown. We conducted a retrospective medical record review study to characterize migraines in the IBD population. Methods: Six hundred seventy-five migraine patients (280 with IBD, 395 without IBD) who were evaluated at Mayo Clinic Rochester, Mayo Clinic Arizona, or Mayo Clinic Florida between July 2009 and March 2021 were included. Patients with ICD codes for migraine and either Crohn’s disease (CD) or ulcerative colitis (UC) were selected. Electronic health care records were reviewed. Patients conﬁrmed to have IBD and migraine were included. Demographic, IBD, and migraine characteristics were collected. Statistical analysis was completed using SAS. Results: Patients with IBD were less often male (8.6% vs 21.3%, P < .001) and had a higher Charlson Comorbidity Index (>2: 24.6% vs 15.7%, P = .003); 54.6% had CD and 39.3% had UC. Patients with IBD had migraine with aura and without aura more frequently ( OR 2.20, P < .001 and OR 2.79, P < .001, respectively) than non-IBD patients. Additionally, those with IBD less commonly had chronic migraine (OR 0.23, P < .001) and less commonly had chronic migraine or treatment for migraine (ORs 0.23-0.55, P ≤ .002). Conclusions: Migraine with and without aura have increased prevalence in IBD patients. Further study of this topic will be helpful to clarify the prevalence of migraine, establish this population’s response to treatment, and better understand the reason(s) for a low rate of treatment.
Dyadic Developmental Psychotherapy for children living with adoptive parents or special guardians: a longitudinal assessment of the impact on child and caregiver wellbeing outcomes
Authors: Burch Katy , Coombes Lindsey , Macey Ellie , Backinsell Andy 
Published: March 2023

Dyadic Developmental Psychotherapy (DDP) is a form of psychotherapy for families where children and young people have experienced early trauma or attachment disruption. This study used longitudinal survey data to understand the format of DDP as it is provided in England for children who are adopted or subject of a Special Guardianship Order (SGO), and to analyse the potential effects of DDP on standardised outcomes of child and caregiver wellbeing. Participants were 150 families who received DDP through funding from the Department for Education’s Adoption Support Fund in England between 2018 and 2021. Adoptive parents and special guardians (caregivers) answered surveys at three timepoints; before therapy was provided (baseline), when therapy had ended (wave 2), and 6 months later (wave 3). Linear mixed modelling demonstrated significant improvements in child/young person difficulties in multiple subscales of the SDQ, including emotional difficulties and conduct problems, between baseline and wave 2. In the same time frame, similar improvements in caregiver wellbeing were also observed, as measured by the SWEMBS. These findings suggest an association between the provision of DDP and an improvement in child/young person and caregiver wellbeing on standardised measures, with these improvements maintained 6 months later. Implications and recommendations for future research to substantiate the findings are discussed.
Discovery of novel glioma serum biomarkers by proximity extension assay
Authors: Ghorbani Atefeh , Avery Lisa M. , Sohaei Dorsa , Soosaipillai Andrea , Richer Maxime , Horbinski Craig , McCortney Katy , Xu Wei , Diamandis Eleftherios P. , Prassas Ioannis 
Published: March 2023

AbstractBackgroundGliomas are among the most malignant tumors, with a very poor prognosis. Early diagnosis is highly desirable since it can help implement more effective treatments for smaller tumors, which have not yet extensively metastasized. Improving early diagnosis may facilitate access of patients to clinical trials and prepare them for the future availability of new disease-modifying treatments.MethodsWe analyzed retrospective samples collected at diagnosis (before therapy initiation), with PEA (Olink Proteomics), quantifying about 3000 proteins. We utilized 30 plasmas from gliomas (20 glioblastomas, 5 anaplastic astrocytomas, 5 anaplastic oligodendrogliomas) and 20 meningiomas (as controls). We then analyzed the data to identify proteins which either alone, or in combination, could discriminate gliomas from meningiomas, or correlate with clinical and molecular alterations.ResultsWe identified 8 plasma proteins which were increased in gliomas vs. meningiomas (GFAP, NEFL, EDDM3B, PROK1, MMP3, CTRL, GP2, SPINT3) and 4 proteins which were decreased in gliomas vs. meningiomas (FABP4, ALDH3A1, IL-12B and OXT). Partition algorithms and logistic regression algorithms with two biomarkers (GFAP and FABP4) achieved sensitivity of 83% and 93% at 100% and 90% specificity, respectively. The strongest single marker was GFAP with an area under the ROC curve (AUC) of 0.86. The AUC for the GFAP-FABP4 combination was 0.98.ConclusionPEA is a powerful new proteomic technology for biomarker discovery. GFAP and a handful of other plasma biomarkers may be useful for early glioma detection and probably, prognosis.StatementDetecting gliomas as early as possible is highly desirable since it can significantly improve the chances of effective treatments. Reliable glioma biomarkers can timely inform glioma patients about the efficacy of their prescribed treatment. Our results reveal some novel putative glioma markers that may prove valuable, when used alone or in combination, towards improved clinical care of gliomas. In order to better appreciate the potential usefulness of these markers, their performance needs to be further validated in a larger cohort of samples.
EnsInfer: a simple ensemble approach to network inference outperforms any single method
Authors: Shen Bingran , Coruzzi Gloria , Shasha Dennis 
Published: March 2023

AbstractThis study evaluates both a variety of existing base causal inference methods and a variety of ensemble methods. We show that: (i) base network inference methods vary in their performance across different datasets, so a method that works poorly on one dataset may work well on another; (ii) a non-homogeneous ensemble method in the form of a Naive Bayes classifier leads overall to as good or better results than using the best single base method or any other ensemble method; (iii) for the best results, the ensemble method should integrate all methods that satisfy a statistical test of normality on training data. The resulting ensemble model EnsInfer easily integrates all kinds of RNA-seq data as well as new and existing inference methods. The paper categorizes and reviews state-of-the-art underlying methods, describes the EnsInfer ensemble approach in detail, and presents experimental results. The source code and data used will be made available to the community upon publication.
multiWGCNA: an R package for deep mining gene co-expression networks in multi-trait expression data
Authors: Tommasini Dario , Fogel Brent L. 
Published: March 2023

Abstract
Background
Gene co-expression networks represent modules of genes with shared biological function, and have been widely used to model biological pathways in gene expression data. Co-expression networks associated with a specific trait can be constructed and identified using weighted gene co-expression network analysis (WGCNA), which is especially useful for the study of transcriptional signatures in disease. WGCNA networks are typically constructed using both disease and wildtype samples, so molecular pathways associated with disease are identified. However, it would be advantageous to study such co-expression networks in their disease context across spatiotemporal conditions, but currently there is no comprehensive software implementation for this type of analysis.

Results
Here, we introduce a WGCNA-based procedure, multiWGCNA, that is tailored to datasets with variable spatial or temporal traits. As well as constructing the combined network, multiWGCNA also generates a network for each condition separately, and subsequently maps these modules between and across designs, and performs relevant downstream analyses, including module-trait correlation and module preservation. When applied to astrocyte-specific RNA-sequencing (RNA-seq) data from various brain regions of mice with experimental autoimmune encephalitis, multiWGCNA resolved the de novo formation of the neurotoxic astrocyte transcriptional program exclusively in the disease setting. Using time-course RNA-seq from mice with tau pathology (rTg4510), we demonstrate how multiWGCNA can also be used to study the temporal evolution of pathological modules over the course of disease progression.

Conclusion
The multiWGCNA R package can be applied to expression data with two dimensions, which is especially useful for the study of disease-associated modules across time or space. The source code and functions are freely available at: https://github.com/fogellab/multiWGCNA.
Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology
Authors: Su Yanting , Guo Yuanyuan , Guo Jieyu , Zeng Ting , Wang Ting , Liu Wu 
Published: March 2023

Abstract
Background
Protein‒protein interactions (PPIs) are the foundation of the life activities of cells. TurboID is a biotin ligase with higher catalytic efficiency than BioID or APEX that reduces the required labeling time from 18 h to 10 min. Since many proteins participate in binding and catalytic events that are very short-lived, it is theoretically possible to find relatively novel binding proteins using the TurboID technique. Cell proliferation, apoptosis, autophagy, oxidative stress and metabolic disorders underlie many diseases, and forkhead box transcription factor 1 (FOXO1) plays a key role in these physiological and pathological processes.

Results
The FOXO1-TurboID fusion gene was transfected into U251 astrocytes, and a cell line stably expressing FOXO1 was constructed. While constructing the FOXO1 overexpression plasmid, we also added the gene sequence of TurboID to perform biotin labeling experiments in the successfully fabricated cell line to look for FOXO1 reciprocal proteins. Label-free mass spectrometry analysis was performed, and 325 interacting proteins were found. A total of 176 proteins were identified in the FOXO1 overexpression group, and 227 proteins were identified in the Lipopolysaccharide -treated group (Lipopolysaccharide, LPS). Wild-type U251 cells were used to exclude interference from nonspecific binding. The FOXO1-interacting proteins hnRNPK and RBM14 were selected for immunoprecipitation and immunofluorescence verification.

Conclusion
The TurboID technique was used to select the FOXO1-interacting proteins, and after removing the proteins identified in the blank group, a large number of interacting proteins were found in both positive groups. This study lays a foundation for further study of the function of FOXO1 and the regulatory network in which it is involved.
Whole transcriptome analysis and construction of a ceRNA regulatory network related to leaf and petiole development in Chinese cabbage (Brassica campestris L. ssp. pekinensis)
Authors: Shi Fengyan , Zhao Zifan , Jiang Yang , Liu Song , Tan Chong , Liu Chuanhong , Ye Xueling , Liu Zhiyong 
Published: March 2023

Abstract
Background
The growth and development of leaves and petioles have a significant effect on photosynthesis. Understanding the molecular mechanisms underlying leaf and petiole development is necessary for improving photosynthetic efficiency, cultivating varieties with high photosynthetic efficiency, and improving the yield of crops of which the leaves are foodstuffs. This study aimed to identify the mRNAs, long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs) related to leaf and petiole development in Chinese cabbage (Brassica campestris L. ssp. pekinensis). The data were used to construct a competitive endogenous RNA (ceRNA) network to obtain insights into the mechanisms underlying leaf and petiole development.

Results
The leaves and petioles of the ‘PHL’ inbred line of Chinese cabbage were used as research materials for whole transcriptome sequencing. A total of 10,646 differentially expressed (DE) mRNAs, 303 DElncRNAs, 7 DEcircRNAs, and 195 DEmiRNAs were identified between leaves and petioles. Transcription factors and proteins that play important roles in leaf and petiole development were identified, including xyloglucan endotransglucosylase/hydrolase, expansion proteins and their precursors, transcription factors TCP15 and bHLH, lateral organ boundary domain protein, cellulose synthase, MOR1-like protein, and proteins related to plant hormone biosynthesis. A ceRNA regulatory network related to leaf and petiole development was constructed, and 85 pairs of ceRNA relationships were identified, including 71 DEmiRNA–DEmRNA, 12 DEmiRNA–DElncRNA, and 2 DEmiRNA–DEcircRNA pairs. Three LSH genes (BrLSH1, BrLSH2 and BrLSH3) with significant differential expression between leaves and petioles were screened from transcriptome data, and their functions were explored through subcellular localization analysis and transgenic overexpression verification. BrLSH1, BrLSH2 and BrLSH3 were nuclear proteins, and BrLSH2 inhibited the growth and development of Arabidopsis thaliana.

Conclusions
This study identifies mRNAs and non-coding RNAs that may be involved in the development of leaves and petioles in Chinese cabbage, and establishes a ceRNA regulatory network related to development of the leaves and petioles, providing valuable genomic resources for further research on the molecular mechanisms underlying leaf and petiole development in this crop species.