bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preserving the Environment Based on the Sunnah of the Prophet: The Road as a Model
Authors: Foda Eman Abdel Aziz Al-Sayed 
Published: February 2023

Preserving the environment is considered one of the most important issues that the Sunnah focused on, as environmental protection has become one of the biggest current problems, The current research dealt with the extent of the Islamic religion’s interest in preserving the environment based on the Sunnah, “The Road as a Model, Through this study, the researcher reached many of the following results and recommendations, which is the call not to extravagance and waste in the use of environmental resources, because this is a form of corruption in the earth, Encouraging the development of the environment by planting, cultivating and preserving the fruitful and non-fruitful plant and tree wealth, And that the rights of the road are a set of rules, demands, and legislative etiquette that came with the Sunnah of the Prophet to regulate people’s relations with the road, its facilities, and its travelers, in terms of obligation or desirability, The Sunnah of the Prophet established a general principle that guarantees the maintenance and preservation of roads, and the care of their rights, Encouraging awareness campaigns to preserve the environment and not harm nature.
Publication activities within the context of scientific policy goals: The bibliometric analysis
Authors: Trofimova I. N. 
Published: February 2023

Science today is the key factor of social development and focus of the government policy. The published and globally acknowledged research results reflect the advance of the national science. The author assesses the publication activity of Russian scientists within the context of the goals of the national sci-tech policy. The newness and originality of the study lies in revealing and analyzing of intrinsic and extrinsic factors of publication activity. The author applied the method of comparing bibliometric indicators to characterize the works by Russian scientists in Scopus database. The goals of the study are: 1) to analyze publication activity structure and dynamics; 2) to define publication impact; and 3) to evaluate competitive strength and potential of publication activity of Russian scientists as compared to foreign researchers. Based on the bibliometric indicators, the author reveals heterodromous trends in Russian science. Traditionally, Russian science maintains its positions in the share of publications in leading journals, with the fast developing life sciences increasing the total number of publications; while the multidisciplinary studies make the most promising vector. The author concludes that despite increasing number of publications and their impact, their contribution to the global science continues to be a relevant problem.
PERSPEKTIF KOMUNIKASI DAN BUDAYA DALAM TRADISI MASYARAKAT JAWA DI KOTA BENGKULU
Authors: Hadiprashada Dhanurseto 
Published: February 2023

Wayang kulit dalam perspektif komunikasi menjadi sebagai sebuah media dalam bentuk seni pertunjukan. Fenomena ini ternyata menjadi salah satu media komunikasi yang efektif ketika pagelaran ini dilakukan setiap selasa kliwon oleh masyarakat Jawa yang ada di Kota Bengkulu. Penelitian ini bertujuan untuk melihat fenomena budaya apa yang muncul dalam pagelaran wayang selasa kliwon di kota Bengkulu ditinjau dalam perspektif komunikasi. Pendekatan yang digunakan adalah pendekatan fenomenologi. Teknik yang digunakan oleh peneliti dalam menentukan informan adalah teknik snow ball (bola salju). Penelitian ini di lakukan pada kelompok masyarakat jawa yang ada di Kota Bengkulu dan merupakan penyelenggara dalam pagelaran wayang kulit selasa kliwon. Hasil Penelitian didapat bahwa wayang kulit merupakan sebuah media yang sangat efektif dalam proses komunikasi dan menciptakan pola hubungan dialektik dalam pertukaran pesan yang terjadi sekaligus menguatkan konsep komunikasi dialogis dalam menjaga keharmonisan komunitas masyarakat Jawa di Kota Bengkulu
Kata kunci : dialogis, kliwon, komunikasi, wayang.
Effects and metabolism of fish collagen sponge in repairing acute wounds of rat skin
Authors: Wang Lei , Qu Yan , Li Wenjun , Wang Kai , Qin Song 
Published: February 2023

Objective: Study the repair effect of tilapia collagen on acute wounds, and the effect on the expression level of related genes and its metabolic direction in the repair process.Materials and methods: After the full-thickness skin defect model was constructed in standard deviation rats, the wound healing effect was observed and evaluated by means of characterization, histology, and immunohistochemistry. RT-PCR, fluorescence tracer, frozen section and other techniques were used to observe the effect of fish collagen on the expression of related genes and its metabolic direction in the process of wound repair.Results: After implantation, there was no immune rejection reaction, fish collagen fused with new collagen fibers in the early stage of wound repair, and was gradually degraded and replaced by new collagen in the later stage. It has excellent performance in inducing vascular growth, promoting collagen deposition and maturation, and re-epithelialization. The results of fluorescent tracer showed that fish collagen was decomposed, and the decomposition products were involved in the wound repair process and remained at the wound site as a part of the new tissue. RT-PCR results showed that, without affecting collagen deposition, the expression level of collagen-related genes was down-regulated due to the implantation of fish collagen.Conclusion: Fish collagen has good biocompatibility and wound repair ability. It is decomposed and utilized in the process of wound repair to form new tissues.
The protective role of EP300 in monocrotaline-induced pulmonary hypertension
Authors: Yang Lei , Tian Jinglin , Wang Jun , Zeng Jie , Wang Ting , Lin Boya , Linneman John , Li Li , Niu Yanqin , Gou Deming , Zhang Yunhui 
Published: February 2023

BackgroundPulmonary hypertension (PH) is a lethal disease characterized by pulmonary vascular remodeling, which is mediated by the abnormal proliferation/migration of pulmonary arterial smooth muscle cells (PASMCs). Recent reports suggest the involvement of histone acetylation in PAH development and that histone deacetylase (HDAC) inhibitors have therapeutic potential for the treatment of PAH. EP300 is an acetyltransferase that plays diverse roles in cell proliferation, differentiation, and apoptosis. However, the functions of EP3000 in PH are rarely studied.ResultsIn this work, we found that the expression of EP300 was increased in the pulmonary arteries of monocrotaline (MCT)-induced PH rats. Knockdown of EP300 by AAV-mediated shRNA exacerbated the PH, with a higher right ventricular systolic pressure (RVSP), right ventricular hypertrophy index (RVHI), and wall thickness in the pulmonary artery of MCT-induced PH rat. On the cellular level, the proliferation of PASMCs was promoted by EP300 knockdown. In addition, the expression of EP300 was increased in PASMCs by the overexpression of EGR1, while the deletion of EGR1 binding sites in the EP300 promoter region decreased the activity of EP300 promoter. Moreover, deleting the EP300 promoter region containing EGR1 binding sites using CRISPR/Cas9 abolished the upregulation of EP300 in MCT-induced rats and exacerbated MCT-induced PH. To summarize, our data indicate that EP300 upregulation mediated by EGR1 has a protective effect on MCT-induced PH.ConclusionThese findings showed EP300 expression was increased in the MCT-induced PH model in rats, which could be mediated by EGR1; the EP300 also displayed the potential to provide protection from PH.
Biventricular circulatory support using single-device and dual-device configurations: Initial pump characterization in simulated heart failure model
Authors: Karimov Jamshid H. , Miyagi Chihiro , Flick Christine R. , Polakowski Anthony R. , Kuban Barry D. , Kuroda Taiyo , Horvath Dennis W. , Fukamachi Kiyotaka , Starling Randall C. 
Published: February 2023

ObjectiveSevere biventricular heart failure (BHF) can be remedied using a biventricular assist device (BVAD). Two devices are currently in development: a universal ventricular assist device (UVAD), which will be able to assist either the left, right, or both ventricles, and a continuous-flow total artificial heart (CFTAH), which replaces the entire heart. In this study, the in vitro hemodynamic performances of two UVADs are compared to a CFTAH acting as a BVAD.MethodsFor this experiment, a biventricular mock circulatory loop utilizes two pneumatic pumps (Abiomed AB5000™, Danvers, MA, USA), in conjunction with a dual-output driver, to create heart failure (HF) conditions (left, LHF; right, RHF; biventricular, BHF). Systolic BHF for four different situations were replicated. In each situation, CFTAH and UVAD devices were installed and operated at two distinct speeds, and cannulations for ventricular and atrial connections were evaluated.ResultsBoth CFTAH and UVAD setups achieved our recommended hemodynamic criteria. The dual-UVAD arrangement yielded a better atrial balance to alleviate LHF and RHF. For moderate and severe BHF scenarios, CFTAH and dual UVADs both created excellent atrial pressure balance. Conversely, when CFTAH was atrial cannulated for LHF and RHF, the needed atrial pressure balance was not met.ConclusionComprehensive in vitro testing of two different BVAD setups exhibited self-regulation and exceptional pump performance for both (single- and dual-device) BHF support scenarios. For treating moderate and severe BHF, UVAD and CFTAH both functioned well with respect to atrial pressure regulation and cardiac output. Though, the dual-UVAD setup yielded a better atrial pressure balance in all BHF testing scenarios.
Research trends in transient receptor potential vanilloid in cardiovascular disease: Bibliometric analysis and visualization
Authors: Zhang Lingfeng , Xu Yantao , Ma Yingxu , Xie Tianjian , Liu Chan , Liu Qiming 
Published: February 2023

BackgroundTransient receptor potential vanilloid (TRPV) is one of the transient receptor potential protein groups; cardiovascular system disease is a crucial cause of mortality among people globally.ObjectiveThis article is intended to accomplish a bibliometric analysis of the trends and public interest since TRPV was reported for the first time.MethodsThe article summarized the Web of Science (WOS) Core Collection on the relationship between TRPV and cardiovascular system disease each year from 2000 to 2021. Data extraction and visualization were completed by R package bibliometrix. Keyword citation burst and co-citation networks were generated and produced by CiteSpace. The map evaluating the distribution of country and region was painted in GunnMap 2 (lert.co.nz). The ranking was performed using the Standard Competition Ranking method. Co-authorship and co-occurrence were analyzed with VOSviewer.ResultsAfter removing duplicated data, books, conference proceedings, and articles of uncertain age, 493 were included, and 17 were excluded. The pattern of publication years showed that the number of publications increased rapidly from 2008 to 2021 with no peak in the number of publications until 2021. The geographical distribution pattern revealed a considerable gap in the number of publications between the United States, China, and other countries, with East Asian institutions leading the world in this area. The pattern of co-authorship showed that 77 institutions were divided into 19 clusters, each covering one country or region.These results suggest that intercontinental cooperation among institutions should be strengthened. The core authors section displayed the change in the most published authors. Keyword analysis listed six burst keywords. Co-citation analysis of references from 2011 to 2021 showed the number and centrality of citations to leading articles.ConclusionOur findings reveal trends and public interest in transient receptor potential vanilloid for cardiovascular disease. These findings suggest that the field has experienced significant growth since 2008, with the United States and China in dominant positions. Our findings also suggest that intercontinental cooperation should be strengthened, and that future research hotspots may focus on pharmacological mechanisms and in-depth exploration of drug clinical trials and new clinical disease application areas such as hypertension, diabetes, and cardiac arrhythmias, which could serve as a foundation for further research.
Poor glycaemic control and ectopic fat deposition mediates the increased risk of non-alcoholic steatohepatitis in high-risk populations with type 2 diabetes: Insights from Bayesian-network modelling
Authors: Waddell T. , Namburete A. , Duckworth P. , Fichera A. , Telford A. , Thomaides-Brears H. , Cuthbertson D. J. , Brady M. 
Published: February 2023

BackgroundAn estimated 55.5% and 37.3% of people globally with type 2 diabetes (T2D) will have concomitant non-alcoholic fatty liver disease (NAFLD) and the more severe fibroinflammatory stage, non-alcoholic steatohepatitis (NASH). NAFLD and NASH prevalence is projected to increase exponentially over the next 20 years. Bayesian Networks (BNs) offer a powerful tool for modelling uncertainty and visualising complex systems to provide important mechanistic insight.MethodsWe applied BN modelling and probabilistic reasoning to explore the probability of NASH in two extensively phenotyped clinical cohorts: 1) 211 participants with T2D pooled from the MODIFY study &amp; UK Biobank (UKBB) online resource; and 2) 135 participants without T2D from the UKBB. MRI-derived measures of visceral (VAT), subcutaneous (SAT), skeletal muscle (SMI), liver fat (MRI-PDFF), liver fibroinflammatory change (liver cT1) and pancreatic fat (MRI-PDFF) were combined with plasma biomarkers for network construction. NASH was defined according to liver PDFF &gt;5.6% and liver cT1 &gt;800ms. Conditional probability queries were performed to estimate the probability of NASH after fixing the value of specific network variables.ResultsIn the T2D cohort we observed a stepwise increase in the probability of NASH with each obesity classification (normal weight: 13%, overweight: 23%, obese: 36%, severe obesity: 62%). In the T2D and non-T2D cohorts, elevated (vs. normal) VAT conferred a 20% and 1% increase in the probability of NASH, respectively, while elevated SAT caused a 7% increase in NASH risk within the T2D cohort only. In those with T2D, reducing HbA1c from the ‘high’ to ‘low’ value reduced the probability of NASH by 22%.ConclusionUsing BNs and probabilistic reasoning to study the probability of NASH, we highlighted the relative contribution of obesity, ectopic fat (VAT and liver) and glycaemic status to increased NASH risk, namely in people with T2D. Such modelling can provide insights into the efficacy and magnitude of public health and pharmacological interventions to reduce the societal burden of NASH.
Heavy metal pollution and risk assessment of tailings in one low-grade copper sulfide mine
Authors: Zhao Pingping , Chen Jinghe , Liu Tianfu , Wang Qiankun , Wu Zengling , Liang Shuqin 
Published: February 2023

Analyzing the pollution level and ecological risk of heavy metals in tailings is a necessary step for conducting revegetation after a tailings pond’s closure. Herein, we determined the heavy metal pollution status and ecological risk in one low-grade copper sulfide tailings pond using chemical and mineralogical analysis, chemical extraction, and ecological risk assessment. The results showed that the low-grade copper sulfide tailings displayed a low pollution status and exhibited a very low ecological risk. Among six heavy metals (Cu, Pb, Zn, As, Cr, and Cd), only Cu (53.7 mg/kg) slightly exceeded its standard value limit (50 mg/kg), and was the main pollutant in the tailings. Due to its high toxicity, As had the maximum contribution to the potential ecological risk in the tailings. Pb, Zn, Cr, and Cd in the tailings were practically of no pollution, and at low or none potential ecological risk. In order to conduct revegetation in the tailings pond, more attention should be paid to the acidity change of tailings and its impact on the chemical activity and bioavailability of Cu and As. This research provides a theoretical basis for heavy metals risk control and revegetation in the low-grade copper sulfide tailings pond.
Genome-wide identification, expression profiling, and functional analysis of ammonium transporter 2 (AMT2) gene family in cassava (Manihot esculenta crantz)
Authors: Xia Jinze , Wang Yu , Zhang Tingting , Pan Chengcai , Ji Yiyin , Zhou Yang , Jiang Xingyu 
Published: February 2023

Background: Nitrogen (N), absorbed primarily as ammonium (NH4+) from soil by plant, is a necessary macronutrient in plant growth and development. Ammonium transporter (AMT) plays a vital role in the absorption and transport of ammonium (NH4+). Cassava (Manihot esculenta Crantz) has a strong adaptability to nitrogen deprivation. However, little is known about the functions of ammonium transporter AMT2 in cassava.Methods: The cassava AMT2-type genes were identified and their characteristics were analyzed using bioinformatic techniques. The spatial expression patterns were analyzed based on the public RNA-seq data and their expression profiles under low ammonium treatment were studied using Real-time quantitative PCR (RT-qPCR) method. The cassava AMT2 genes were transformed into yeast mutant strain TM31019b by PEG/LiAc method to investigate their functions.Results: Seven AMT2-type genes (MeAMT2.1-2.7) were identified in cassava and they were distributed on 6 chromosomes and included two segmental duplication events (MeAMT2.2/MeAMT2.4 and MeAMT2.3/MeAMT2.5). Based on their amino acid sequences, seven MeAMT2 were further divided into four subgroups, and each subgroup contained similar motif constitution and protein structure. Synteny analysis showed that two and four MeAMT2 genes in cassava were collinear with those in the Arabidopsis and soybean genomes, respectively. Sixteen types of cis-elements were identified in the MeAMT2 promoters, and they were related to light-, hormone-, stress-, and plant growth and development-responsive elements, respectively. Most of the MeAMT2 genes displayed tissue-specific expression patterns according to the RNA-seq data, of them, three MeAMT2 (MeAMT2.3, MeAMT2.5, and MeATM2.6) expressions were up-regulated under ammonium deficiency. Complementation experiments showed that yeast mutant strain TM31019b transformed with MeAMT2.3, MeAMT2.5, or MeATM2.6 grew better than untransgenic yeast cells under ammonium deficiency, suggesting that MeAMT2.3, MeAMT2.5, and MeATM2.6 might be the main contributors in response to ammonium deficiency in cassava.Conclusion: This study provides a basis for further study of nitrogen efficient utilization in cassava.
Control of Mycobacterium avium subsp. paratuberculosis load within infected bovine monocyte-derived macrophages is associated with host genetics
Authors: Badia-Bringué Gerard , Canive María , Alonso-Hearn Marta 
Published: February 2023

The genetic loci influencing individual resistance to Mycobacterium avium subsp. paratuberculosis (MAP) infection are still largely unknown. In the current study, we searched for genetic loci associated with resistance to MAP infection by evaluating the performance of monocyte-derived macrophages (MDMs) isolated from the peripheral blood of 75 healthy Holsteins cows and infected ex vivo with MAP. Bacterial load (log colony-forming units, log CFUs) within MDMs was quantified at 2 h and 7 days p. i. using a BACTEC MGIT 960 instrument. In addition, the expression levels of some genes with important roles in the innate immune response including epiregulin (EREG), complement component C3 (C3), galectin-9 (Gal9), and nitric oxide (NO-) were measured in the supernatant of the infected cells. DNA from peripheral blood samples of the animals included in the study was isolated and genotyped with the EuroG MD bead Chip (44,779 single nucleotide-polymorphisms, SNPs). Linear mixed models were used to calculate the heritability (h2) estimates for each indicator of MDM performance, MAP load within MDMs and EREG, C3, Gal9, and NO-expression. After performing a genome-wide association study, the only phenotypes that showed SNPs with a significant association were the bacterial load within MDMs at 2 h (h2 = 0. 87) and 7 days (h2 = 0.83) p.i. A total of 6 SNPs, 5 candidate genes, and one microRNA on the Bos taurus chromosomes BTA2, BTA17, BTA18, and BTA21 were associated with MAP load at 2 h p.i. Overlap was seen in two SNPs associated with the log CFUs at 2 h and 7 d p.i. The identified SNPs had negative regression coefficients, and were, therefore, associated with a low bacterial load within MDMs. Some of the identified SNPs were located within QTLs previously associated with longevity, reproductive, and udder health traits. Some of the identified candidate genes; Oxysterol Binding Protein Like 6, Cysteine and Serine Rich Nuclear Protein 3, and the Coiled-Coil Domain Containing 92 regulate cellular cholesterol trafficking and efflux, apoptosis, and interferon production, respectively. Taken together, our results define a heritable and distinct immunogenetic profile in MAP-infected macrophages designed to limit bacterial load early after infection.
Developing an m5C regulator–mediated RNA methylation modification signature to predict prognosis and immunotherapy efficacy in rectal cancer
Authors: Zhang Rixin , Gan Wenqiang , Zong Jinbao , Hou Yufang , Zhou Mingxuan , Yan Zheng , Li Tiegang , Lv Silin , Zeng Zifan , Wang Weiqi , Zhang Fang , Yang Min 
Published: February 2023

BackgroundCurrently, a very small number of patients with colorectal cancer (CRC) respond to immune checkpoint inhibitor (ICI) treatment. Therefore, there is an urgent need to investigate effective biomarkers to determine the responsiveness to ICI treatment. Recently, aberrant 5-methylcytosine (m5C) RNA modification has emerged as a key player in the pathogenesis of cancer. Thus, we aimed to explore the predictive signature based on m5C regulator–related genes for characterizing the immune landscapes and predicting the prognosis and response to therapies.MethodsThe Cancer Genome Atlas (TCGA) cohort was used as the training set, while GEO data sets, real-time quantitative PCR (RT-qPCR) analysis from paired frozen tissues, and immunohistochemistry (IHC) data from tissue microarray (TMA) were used for validation. We constructed a novel signature based on three m5C regulator–related genes in patients with rectal adenocarcinoma (READ) using a least absolute shrinkage and selection operator (LASSO)-Cox regression and unsupervised consensus clustering analyses. Additionally, we correlated the three-gene signature risk model with the tumor immune microenvironment, immunotherapy efficiency, and potential applicable drugs.ResultsThe m5C methylation–based signature was an independent prognostic factor, where low-risk patients showed a stronger immunoreactivity phenotype and a superior response to ICI therapy. Conversely, the high-risk patients had enriched pathways of cancer hallmarks and presented immune-suppressive state, which demonstrated that they are more insensitive to immunotherapy. Additionally, the signature markedly correlated with drug susceptibility.ConclusionsWe developed a reliable m5C regulator–based risk model to predict the prognosis, clarify the molecular and tumor microenvironment status, and identify patients who would benefit from immunotherapy or chemotherapy. Our study could provide vital guidance to improve prognostic stratification and optimize personalized therapeutic strategies for patients with rectal cancer.