bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Sox9 in BPD and its effects on the Wnt/β-catenin pathway and AEC-II differentiation
Authors: Xu Wei , Wu Di , Bai Dongqin , Yang Miao , Wu Bo 
Published: August 2023

Abstract
The excessive activation of the Wnt/β-catenin signaling pathway is an important regulatory mechanism that underlies the excessive proliferation and impaired differentiation of type 2 alveolar epithelial cells (AEC-II) in bronchopulmonary dysplasia (BPD). Sox9 has been shown to be an important repressor of the Wnt/β-catenin signaling pathway and plays an important regulatory role in various pathophysiological processes. We found that the increased expression of Sox9 in the early stages of BPD could downregulate the expression of β-catenin and promote the differentiation of AEC-II cells into AEC-I, thereby alleviating the pathological changes in BPD. The expression of Sox9 in BPD is regulated by long noncoding RNA growth arrest-specific 5. These findings may provide new targets for the early intervention of BPD.
MiR-23b and miR-133 cotarget TGFβ2/NOTCH1 in dermal fibroblasts and affect hair follicle development in sheep
Authors: He Junmin , Wei Chen , Huang Xixia , Zhang Guoping , Mao Jingyi , Li Xue , Yang Cunming , Zhang Wenjing , Tian Kechuan , Liu Guifen 
Published: August 2023

Abstract
Background
Wool, which is a pure natural textile fiber raw material, has high economic value and is increasingly favored by modern textile enterprises and consumers. The output of fine wool, especially high-quality superfine wool, in China is far from meeting the processing needs of the textile market. Therefore, improving the yield and quality of wool has always been a focus of research related to fine wool sheep breeding. Wool is produced and controlled by hair follicles (HFs). However, little is known about the mechanisms involved in HF development and regulation. The structure, function and morphogenesis of HFs are complex biological processes. Sheep dermal fibroblasts (SDFs) play a key role in the initial stage of HF development. Analyzing the molecular mechanism that regulates early HF development in superfine wool sheep is of great importance for better understanding the HF morphogenesis process and for the breeding of fine wool sheep. Here, we show that two microRNAs (miRNAs) affect the development of HFs by targeting two genes that are expressed by SDFs.
Results
On the basis of our previous research, we further found that oar-miR-23b and oar-miR-133 and their cotarget genes TGFβ2 and NOTCH1 were differentially expressed during the six stages of HF development in superfine wool sheep. Overexpression/inhibition of oar-miR-23b and oar-miR-133 in SDFs showed that oar-miR-23b and oar-miR-133 inhibited the proliferation and migration of SDFs and promoted the apoptosis of SDFs through TGFβ2 and NOTCH1. By analyzing genes that are upstream and downstream of the target genes, we found that oar-miR-23b and oar-miR-133 affected the expression of genes in the TGF-β, Hippo and WNT signaling pathways.
Conclusion
oar-miR-23b and oar-miR-133 inhibit the proliferation and migration of SDFs by jointly targeting TGFβ2 and NOTCH1, thereby inhibiting the development of super fine wool HFs. Our research provides a molecular marker that can be used to guide the breeding of ultrafine wool sheep.
The role of magma ascent rates and viscosity in the explosive and dome eruptions (Kelud volcano, Indonesia)
Authors: Utami Sri Budhi , Costa Fidel , Humaida Hanik 
Published: August 2023

Abstract
Magma ascent rate can control the hazard potential of an eruption, but it is difficult to directly determine. Here we investigate the variations in timescales of magma ascent and rates of magma ascent for the three most recent explosive and effusive eruptions of Kelud volcano in Indonesia (1990, 2007, and 2014) using the zoning of volatile elements (OH, Cl, F) in apatite. We found that crystals from the 2007 dome show chemical gradients and increasing concentrations (reverse zoning) in chlorine and/or fluorine towards the crystals’ rims whereas those of the 1990 and 2014 explosive eruptions are unzoned. Diffusion modelling of the volatile elements in zoned apatite of the 2007 dome rocks give magma ascent times of up to 3 months, although 71% of them are ≤ 60 days. In contrast, the maximum magma ascent timescales inferred from apatite of the 1990 and 2014 explosive eruptions are about 7–8 hours. Using the pre-eruptive magma storage depths obtained from petrological and phase equilibria studies, we calculate ascent rates of about > 0.4 × 10− 3 m s− 1 for the 2007 dome, and > 2.6 × 10− 1 m s− 1 for the 1990 and 2014 eruptions. We also calculated the magma viscosities for each eruption, which when combined with the magma ascent rates and magma mass discharge rates correspond well with the expected eruptive styles. Our study illustrates the robustness of modelling apatite zoning in volatile elements to constrain timescales and magma ascent dynamics, and highlights the important role of magma ascent on eruptive styles.
A Liquid Biopsy To Detect Transcriptionally Active Human Papillomavirus 16 From Patient Saliva
Authors: Deutsch Fiona , Sais Dayna , Hill Meredith , Tran Ngoc Ha , Elliott Michael , Tran Nham 
Published: August 2023

Abstract
Head and neck cancers, particularly oropharyngeal cancers (OPC), have been increasingly associated with human papillomavirus (HPV) infections, specifically HPV16. The current methods for HPV16 detection primarily rely on p16 staining or PCR techniques. However, it is important to note the limitations of conventional PCR, as the presence of viral DNA does not always indicate an ongoing viral infection. Moreover, these tests heavily rely on the availability of tissue samples, which can present challenges in certain situations. In this study, we developed a qPCR multiplex approach to detect HPV16 oncogenes E6 and E7 RNA in saliva samples from OPC patients. Salivary supernatant was used as the liquid biopsy source. We successfully obtained RNA from salivary supernatant, preserving its integrity as indicated by the detection of several housekeeping genes. Our multiplex approach accurately detected E6 and E7 RNA in HPV16-positive cell lines, tissues, and finally in OPC salivary samples. Importantly, the assay specifically targeted HPV16 and not HPV18. This multiplexing technique allowed for reduced sample input without compromising specificity. The multiplex qPCR approach presented in this study demonstrates the potential in accurately detecting viable HPV16 in saliva and tissue samples from OPC patients. Since the assay measures HPV16 RNA, it provides insights into the transcriptional activity of the virus. This could guide clinical decision-making and treatment planning for individuals with HPV-related OPC.
Mediation of the Association between Social Environmental Characteristics of Family Childcare Home and Weight Status in Children by Diet Quality
Authors: Jiang Qianxia , Risica Patricia Markham , Tovar Alison , Stowers Kristen Cooksey , Schwartz Marlene B. , Lombardi Caitlin , Gans Kim M. 
Published: August 2023

Abstract
Background: The food and beverages served in family childcare homes (FCCHs) may play an important role in the development of childhood overweight and obesity. This analysis examines whether children’s diet quality mediates the relationship between foods and beverages served in FCCHs and preschool-aged children’s weight status.
Methods: Trained and certified staff conducted observations for two days in each FCCH, using the Environment and Policy Assessment and Observation (EPAO) measure to determine the foods and beverages served to children (N=370) in FCCHs (N=120). They also used the Dietary Observation in Child Care (DOCC) protocol to assess children’s food and beverage intake during childcare, from which we calculated the Healthy Eating Index-2015 (HEI), a measure of diet quality. Height and weight were measured for each child with parent consent from which the child’s body mass index (BMI) z-scores were calculated from. A multilevel mediation analysis was conducted to indicate whether children’s diet quality mediates the relations between food and beverage served in FCCHs and preschool-aged children’s weight status.
Results: Children’s total HEI scores significantly mediated the relationship between the EPAO subscale “Food provided” and children’s BMI z-scores (B=-.01, p<.05, 95% CI = [-.03, -.002]). Further, the EPAO subscale “Food provided” was positively associated with the total HEI score (B=.75, p<.01, 95% CI = [.32, 1.18]). Total HEI scores were negatively associated with BMI z-score (B=-.01, p<.05, 95% CI = [-.02, -.001]).
Conclusion: Children’s diet quality did significantly mediate the relationship between the food served in FCCHs and children’s weight status. More longitudinal studies with longer follow-up periods need to be conducted to confirm these relationships. Further, future studies need to examine the relationships between a broader spectrum of FCCH environmental characteristics and home environment with children’s weight status, as well as other mediators including physical activity.
Association between lactate-to-albumin ratio and 28-days all- cause mortality in patients with Sepsis-associated liver injury: A Retrospective Cohort Study
Authors: Yi Xiaona , Jin Dongcai , Huang Shanshan , Xie Zhenye , Zheng Meixia , Zhou Fen , Jin Yuhong 
Published: August 2023

Abstract
Background
This study aimed to clarify the correlation between LAR and 28-day all-cause mortality in patients with sepsis-associated liver injury (SALI).
Methods
In this retrospective cohort study, data were obtained from the Medical Information Mart for Intensive Care IV (v2.2). Adult patients with SALI were admitted to the intensive care unit in this study. The LAR level at admission was included, and the primary aim was to assess the relationship between the LAR and 28-day all-cause mortality.
Results
A total of 341 patients with sepsis-associated liver injury (SALI) were screened. They were divided into a survival group (241) and a non-survival group (100), and the 28-day mortality rate was 29.3%. After adjusting for confounding factors, patients in the highest LAR quartile had an increased risk of death compared with those in the lowest LAR quartile (HR, 2.87; 95% CI: 1.11–7.42, p for trend, 0.012), using LAR as a categorical variable. When LAR was presented as a continuous variable, the prevalence of in-hospital sepsis-related deaths increased by 21% (adjusted HR: 1.21, 95% CI: 1.07–1.37, P = 0.002) for each 1- unit increase in LAR, regardless of confounders. Stratification and sensitivity analyses indicated that the results are robust.
Conclusions
The 28-day all-cause mortality rate in patients with Sepsis-associated liver injury (SALI) was positively correlated with the LAR levels.
Meta-hydrodynamic routes to viscous electron flow
Authors: Estrada-Alvarez Jorge , Dominguez-Adame Francisco , Díaz Elena 
Published: August 2023

Abstract

Viscous flow of interacting electrons in two dimensional
materials features a bunch of exotic effects. A model resembling the Navier-Stokes equation for classical fluids accounts for them in the so called hydrodynamic regime. This regime occurs when electron-electron collisions are frequent enough. We performed a detailed analysis of the hydrodynamic requirements and found three new routes to achieve viscous electron flow: favouring frequent inelastic collisions, the application of a magnetic field or a high-frequency electric field. Our results show that the conventional requirement of frequent electron-electron collisions is too restrictive and, as a consequence, materials and phenomena to be described using hydrodynamics are widened. We discuss recent experiments regarding Poiseuille-like flows, superballistic conduction and negative resistances as signatures for viscous flow onset. We conclude that these usual signatures of viscous electron flow are achieved by following alternative meta-hydrodynamic routes.
The Residency Levels Effect on Pediatric Dental Rehabilitation Operation Time
Authors: Baakdah Raniah , Al-Hinidi Mohammed , Al-Sharif Shrouq , Al-Kharouby Shahad , Al-Qarni Raghad , Al-Sulami Yara , Al-Nakhli Rabab 
Published: August 2023

Abstract
Background
General anesthesia (GA) is the most frequently used pharmacological intervention in pediatric dental care. Postgraduate pediatric dental residents must perform a variety of pediatric dental rehabilitation procedures under GA at different levels of training. An adequate operation time (OT) decreases morbidity risk and improves hospital time utilization efficiency. The aim of the study is to assess the effect of pediatric dental resident training level on OT for pediatric dental rehabilitation procedures under GA.
Methods
A cross-sectional study of data from all pediatric dental rehabilitation procedures that were performed under GA by pediatric dental residents at KAMC-Jeddah from 2015 to 2022 was conducted. The primary outcome was OT, and the predictive variable was resident training levels. A linear regression analysis was used to compare OT between procedures performed by junior (years 1–2) or senior (years 3–4) trainees, adjusting for patient and operative factors.
Results
A total of 1007 pediatric dental rehabilitation cases were performed under GA by junior (25%) and senior (65%) residents. The univariant analysis indicated that senior residents had significantly longer OTs (13 minutes) than junior residents. However, after considering significant variables, the linear regression analysis showed that senior residents had a significantly shorter OT and performed significantly more dental procedures than junior residents. Senior residents took significantly more radiographs and performed significantly more primary pulp therapies and multisurface anterior colored restorations under GA than junior residents.
Conclusions
The OT for pediatric dental rehabilitation procedures under GA is associated with resident training level. The total operation time was significantly longer based on procedure number, type, and resident level. This finding highlights the importance of assigning procedures that require GA to residents based on their level and the complexity of the case. Additionally, it evaluates residents’ competency development and assesses the impact of residency training programs on hospital efficiency.
Trial registration:
The KAIMRC Internal Review Board (IRB/1514/22) registered this cross-sectional chart review.
Association of elevated serum urea nitrogen and the remaining renal function indicators with recurrence of acute ischaemic stroke, with mediating and interacting effects with blood inflammatory indicators
Authors: wang yan , Zhang Mengli , Zhu Hanyu , Pan Zhiming , Chen Jiahui , He Haijun , 王 贞 
Published: August 2023

Abstract
Background and purpose: The relationship between blood urea nitrogen (BUN) and other markers of renal function and the recurrence of acute ischemic stroke (AIS) have been scarcely reported. Inflammatory mechanisms may play a role in these relationships.The aims of this study were to investigate the relationship between BUN and recurrence of AIS and to explore the roles of inflammatory indicators in these relationships.
Methods: A total of 10,973 patients hospitalised for cerebral infarction from 2015 to 2020 were included in this study. BUN, creatinine (CR), uric acid (UA), BUN/CR, UA/CRand BUN/UA were divided into four groups. Cox regression and restricted three-sample bar regression models were used to evaluate the clinical correlations between BUN and other indicators of renal function and ischaemic stroke recurrence.
Results:Among the indicators, the risk of ischaemic stroke recurrence was increased by 117.0% (hazard ratios (HR)= 2.170, 95% confidence interval (CI)=1.816–2.593, P<0.001) in patients with the fourth quartile BUN level (Q4 ≥ 6.4 mmol/L), using the first quartile level (Q1<4.3 mmol/L) as the reference value. BUN predicted a greater risk ratio of ischaemic stroke recurrence over 5 years than did the remaining indicators of renal function. We also found that inflammatory markers play a mediating role.
Conclusions:Higher BUN levels were associated with recurrent ischaemic stroke events within 5 years. And, the inflammatory markers white blood cell (WBC) count, absolute neutrophil count (ANC), absolute lymphocyte count (ALC) and neutrophil to lymphocyte ratio (NLR) mediated and interacted with BUN and ischaemic stroke recurrence.
Measuring Performance of Ensemble of Ensembles (EoE) Models
Authors: Attia¹ Mazza Yousuf , Noureldien Noureldien A. 
Published: August 2023

Abstract
To improve the performance of machine-learning algorithms irrelevant and redundant features must be removed. Accordingly, many different feature selection methods have been proposed to analyze the high-dimensional datasets and determine subsets of relevant features. Ensemble feature selection methods have been proposed to integrate the advantages of single-feature selection methods. The ensemble feature selection concept is based on aggregating the outputs of the different feature selection methods into a single output. Many studies have shown that feature subsets generated by ensemble methods improve the performance of machine learning models more than single feature selection methods. In this study, we extended the concept of ensemble feature selection to the concept of ensemble of ensembles of feature selection, where the outputs of different ensembles are aggregated into a single output. Through comprehensive experimental tests, we study the effect of the new concept on the performance of machine-learning models. The results show that the ensemble of ensembles does not guarantee the improvement of the performance of the learning models in comparison to individual ensembles. Thus, the complexity of the structure of an ensemble of ensembles makes it an unworthy approach for improving model performance.
Bioinformatics analysis of the Panax ginseng cyclophilin gene and its anti-Phytophthora cactorum activity
Authors: Yu Zhao , Lu Jiahong , Hao Kaiwen , Wang Yuming , Liu Zhimei , Li Shuaijun , Xue Dongming , Xiao Lizhi , Duan Qidong , Zhang Miao , Hui Fang , Qin Huacong , Lian Wenhui , Tianxia Sun 
Published: August 2023

Abstract
In this paper, Panax ginseng cyclophilin (PgCyP) was successfully obtained through a genetic engineering technique. A bioinformatics method was used to analyze the physicochemical properties and structure of PgCyP. The results showed that PgCyP belongs to the cyclophilin gene family. The protein encoded by the PgCyP gene contains the active site of PPIase (R62, F67, H133) and a binding site for cyclosporine A (W128). The relative molecular weight of PgCyP is 187.11 kDa, the theoretical isoelectric point is 7.67, and it encodes 174 amino acids. The promoter region of PgCyP mainly contains the low-temperature environmental stress response element (LTR), abscisic acid-responsive cis-acting element (ABRE), and light-responsive cis-acting element (G-Box). PgCyP includes a total of nine phosphorylation sites, comprising 4 serine phosphorylation sites, 3 threonine phosphorylation sites, and 2 tyrosine phosphorylation sites. PgCyP was recombined and expressed in vitro, and its recombinant expression was investigated. Furthermore, it was found that the recombinant PgCyP protein could effectively inhibit the germination of spores and the normal growth of mycelium in vitro. Further experiments on the roots of susceptible Arabidopsis thaliana showed that the PgCyP protein could improve the resistance of Arabidopsis to Phytophthora cactorum. The findings of this study provide a basis for the use of the PgCyP protein as a new type of green biopesticide.
Locus Coeruleus is associated with higher openness to experience and IQ: implications for the noradrenergic system for novelty seeking in daily life
Authors: Plini Emanuele , Robertson Ian , Brosnan Meadhbh , Dockree Paul 
Published: August 2023

Abstract
Novelty exposure and the upregulation of the noradrenergic (NA) system have been suggested as crucial for developing cognitive reserve and resilience against neurodegeneration. Openness to Experience (OE), a personality trait associated with interest in novel experiences, may play a key role in facilitating this process. High-OE individuals tend to be more curious and encounter a wider range of novel stimuli throughout lifespan. To investigate the relationship between OE and the main core of the noradrenergic system, the Locus Coeruleus (LC), as well as its potential mediation of IQ—a measure of cognitive reserve—MRI structural analyses were conducted on 135 healthy young adults. Compared to other neuromodulators' seeds, such as Dorsal and Median Raphe (DR-MR) -5-HT, Ventral Tegmental Area (VTA) -DA-, and Nucleus Basalis of Meynert (NBM) -Ach-, the results indicated that higher LC signal intensity correlated with greater OE and IQ. Furthermore, mediation analyses revealed that only LC played a mediating role between OE and IQ. These findings shed light on the neurobiology of personality and emphasize the importance of LC-NA system integrity in novelty-seeking behavior. They provide a psychobiological explanation for how OE expression can contribute to the maintenance of the noradrenergic system, enhancing cognitive reserve and resilience against neurodegeneration.
Superconductivity in a breathing kagomé metals ROs2 (R = Sc, Y, Lu)
Authors: Górnicka Karolina , Winiarski Michał J. , Walicka Dorota I. , Klimczuk Tomasz 
Published: August 2023

Abstract
We have successfully synthesized three osmium-based hexagonal Laves compounds ROs2 (R = Sc, Y, Lu), and discussed their physical properties. LeBail refinement of pXRD data confirms that all compounds crystallize in the hexagonal centrosymmetric MgZn2 – type structure (P63/mmc, No. 194). The refined lattice parameters are a = b = 5.1791(1) Å and c = 8.4841(2) Å for ScOs2, a = b = 5.2571(3) Å and c = 8.6613(2) Å for LuOs2 and a = b = 5.3067(6) Å and c = 8.7904(1) Å for YOs2. ROs2 Laves phases can be viewed as a stacking of kagomé nets interleaved wih triangular layers. Temperature-dependent magnetic susceptibility, resistivity and heat capacity measurements confirm bulk superconductivity with Tc = 5.36, 4.55, and 3.47 K for ScOs2, YOs2, and LuOs2, respectively. We have shown that all investigated Laves compounds are weakly-coupled type-II superconductors. DFT calculations revealed that the band structure of ROs2 is intricate due to multiple interacting d orbitals of Os and R. Nonetheless, the kagomé-derived bands maintain their overall shape, and the Fermi level is found originates from the kagomé flat bands that are broadened by interlayer interaction. As a result, ROs2 can be classified as (breathing) kagomé metal superconductors.
Design and Analysis of a Novel 6-DOF Lorentzian Pointing Platform for Mass Transfer
Authors: Wang Wei , Liu Qian , Huang Yan , Cao YingYu , Liu Qiang 
Published: August 2023

Abstract
To improve the efficiency of mass transfer, a novel magnetic levitation platform is invented. A three-degree-freedom Lorentz bearing is used to realize 6-degree-freedom. The structure and work principle of the three-degree-freedom Lorentz bearing are introduced. A novel circular Halbach array planar motor is used to achieve axial and radial movement. The force model of the magnetic bearing and the planar motor are established. To improve the magnetic flux density at the air gap, a novel Halbach permanent magnetic array is invented. The model of the magnetic flux density is established. The finite element analysis method is used to analyze the magnetic flux density at the air gap. The maximum magnetic flux density is 385mT, which is 375mT in the normal array. An experimental prototype is manufactured. The stroke of the straight line is ± 2mm×±2mm×±1.5mm and the rotation stroke is ± 0.5°×±0.5°×±3°, which is improved about 1.5° in traditional magnetic levitation platform.