bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between grip strength and stress urinary incontinence of NHANES 2011–2014
Authors: Zhang Nieke , Mao Weipu , Sun Si , Zhang Guanyuan , Shi Naipeng , Yao Chi , Liu Ning , Chen Shuqiu , Gao Wei , Zhang Lei , Chen Ming , Zou Xiangyu 
Published: July 2023

Abstract
Objectives: To investigate the association between grip strength (GS) and relative grip strength (rGS) with the prevalence and severity risk of SUI.
Methods: Female patients were retrieved from the National Health and Nutrition Examination Survey (NHANES) 2011-2014. GS was measured using a digital hand dynamometer, rGS was calculated based on GS. Chi-square test was adopted to analyze the categorical variables, and t-test for slope was performed in generalized linear models for the continuous variables. The potential bias of baseline variables between different groups was controlled by performing the propensity score matching (PSM). Logistic regression models were established to detect the relationship between GS or rGS and the prevalence or severity risk of SUI before and after PSM.
Results: A total of 4263 participants were included, with 3085 (85%) people in SUI group and 1178 (27.6%) people in non-SUI group. rGS level of people without SUI (2.06, 0.57) was higher than that of SUI patients (1.75, 0.54). Monthly SUI patients' rGS level (1.81, 0.55) was higher than weekly SUI patients' level (1.57, 0.46). A similar result was observed for GS. Logistic regression analysis showed that risks of prevalence and severity of SUI decreased with increasing levels of GS and rGS. rGS was found to have a stronger association with SUI than GS [prevalence: GS: Q4 vs. Q1: aOR=0.633, 95%CI=0.508-0.789, p<0.001; rGS: Q4 vs. Q1: aOR=0.365, 95%CI=0.290-0.459, p<0.001; severity: GS: Q4 vs. Q1: aOR=0.727, 95%CI=0.600-0.881, p=0.001; rGS: Q4 vs. Q1: aOR=0.371, 95%CI=0.282-0.488, p<0.001]. The results of PSM confirmed that GS and rGS were correlated with SUI.
Conclusions: Lower levels of GS and rGS are associated with an increased prevalence and severity risk of SUI, with rGS having a stronger association than GS.
A near real time flood hazard assessment tool using Satellite Data and Artificial Intelligence
Authors: Gemitzi Alexandra , Kopsidas Odysseas , Stefani Foteini , Polymeros Apostolos , Bellos Vasilis 
Published: July 2023

Abstract
This work aims to develop a constantly updated flood hazard assessment tool that utilizes readily available datasets. It is based on the recently released global land use/land cover (LULC) dataset Dynamic World, which is readily available as an open data source within Google Earth Engine platform. It is updated constantly following the release rate of Senitnel-2 images, i.e., every 2 to 5 days depending on the location. The tool presented herein identifies the number of times each 10 m pixel is characterized as flooded for a selected time period, and it is updated accordingly with the new flood events, thus providing a near real time detection of flooded areas. The detected areas are compared against the Regional Flood Hazard Maps developed in the frames of the EU Water Framework Directive, and several inconsistencies of the two approaches were highlighted and commented on. Besides, a new variable is proposed, named Maximum Potential Economic Loss is introduced in order to assess the socioeconomic implications. The presented methodology demonstrates how remotely sensed based datasets, becoming available at increasing rates, can be used as an operational instrument in order to define and update the flood hazard zones required for water resources management plans and policy makers. Our results indicated the usefulness of the complementary use of the conventional flood hazard mapping approaches with those based on emerging techniques and datasets.
Degraded inferior colliculus responses to complex sounds in prenatally exposed VPA rats
Authors: Tamaoki Yuko , Pasapula Varun , Chandler Collin , Borland Michael S. , Olajubutu Olayinka I. , Tharakan Liza S. , Engineer Crystal T. 
Published: July 2023

Abstract
Background
Individuals with autism spectrum disorders (ASD) often exhibit altered sensory processing and deficits in language development. Prenatal exposure to valproic acid (VPA) increases the risk for ASD and impairs both receptive and expressive language. Like individuals with ASD, rodents prenatally exposed to VPA exhibit degraded auditory cortical processing and abnormal neural activity to sounds. Disrupted neuronal morphology has been documented in earlier processing areas of the auditory pathway in VPA-exposed rodents, but there are no studies documenting early auditory pathway physiology. Therefore, the objective of this study is to characterize inferior colliculus (IC) responses to different sounds in rats prenatally exposed to VPA compared to saline-exposed rats.
Methods
Neural recordings from the inferior colliculus were collected in response to tones, speech sounds, and noise burst trains.
Results
Our results indicate that the overall response to speech sounds was degraded in VPA-exposed rats compared saline-exposed controls, but responses to tones and noise burst trains were unaltered.
Conclusions
These results are consistent with observations in individuals with autism that neural responses to complex sounds, like speech, are often altered, and lays the foundation for future studies of potential therapeutics to improve auditory processing in the VPA rat model of ASD.
Single-cell combined transcriptional sequencing to explore key genes and regulatory mechanisms in diabetic retinopathy
Authors: Li Fei , Xiang Qingping , Hou Peixian , Dai Yan , Liu Longqian 
Published: July 2023

Abstract
Background
Diabetic retinopathy (DR), a prevalent complication of diabetes with a poor prognosis, remains incompletely understood. Therefore, an in-depth study on the pathogenesis of DR at the molecular level is essential to identify key DR-related genes. The objective of this study was to employ bioinformatics approaches to explore key genes and potential molecular mechanisms underlying DR.
Results
The single-cell sequencing dataset (GSE209872) and transcriptome sequencing datasets (GSE94019 and GSE102485) from the GEO database were utilized to screen for differentially expressed genes. Through WGCNA analysis and GSEA enrichment analysis, key genes and potential mechanisms were identified. Six key genes associated with the development of DR, namely CD44, CPLX4, MMP14, PMEPA1, PMP22, and POSTN were screened, and the specific signaling mechanisms associated with the key genes causing DR were predicted. To assess the immune infiltration, the CIBERSORT method was employed. The immune profiling revealed significant heterogeneity in immune response between the control group and the DR group.
Conclusions
These six key genes have the potential to become biomarkers for the diagnosis of DR and provide new targets and research directions for the treatment of DR.
Evaluation of the Separate and Combined Effects of Anodal tDCS over the M1 and F3 Regions on Pain Relief in Patients with Type-2 Diabetes Suffering from Neuropathic Pain
Authors: Alipour Ahmad , Mohammadi Roghayeh 
Published: July 2023

Abstract
Neuropathic pain (NP) is a common complication of chronic diabetes that negatively affects the routine functioning and sleep of patients. The present study aimed to investigate the separate and combined effects of anodal transcranial direct current stimulation (tDCS) over the primary motor cortex (M1) and left dorsolateral prefrontal cortex (F3) regions on pain relief in patients with type-2 diabetes suffering from NP. The statistical population of this double-blind randomized clinical trial consisted of all the members of the Bonab Diabetes Association in 2022 aged 45 to 65 years who were diagnosed with NP by a specialist. A total of 48 patients who met the inclusion criteria were selected as the sample through purposive sampling. The participants were then randomly assigned into 4 groups, each attending 12 sessions of a special intervention (three times a week). The Short Form-McGill Pain Questionnaire-2 (SF-MPQ-2) was used for data collection. Data were statistically analyzed using SPANOVA, analysis of covariance, and the Bonferroni test. The results showed that perceived pain intensity significantly changed in all groups over time (F=11.48, P<0.001). The mean perceived pain intensity in the posttest was significantly lower in the M1 stimulation group than in the F3 stimulation group. Nevertheless, there was no significant difference between the two groups in terms of perceived pain intensity in the one-month and two-month follow-up stages. Considering its therapeutic potential for pain relief, the application of tDCS (over both M1 and F3) can be expanded for diabetic patients.
Detection of GLUT-1, Hexokinase-Ii, Ki-67, P53, and P16 Levels and HPV With 18F-Fdg PET/CT in Penile Cancer
Authors: SALAZAR ANDRE LOPES , Júnior Eduardo Paulino , SANCHEZ DIEGO F. , Simoes Renata Toscano , Gonçalves Aizis Tatiane Santos , SILVA-FILHO Raul , RAIMUNDO JOAO V S , SOARES YURI V C , MARINHO MATHEUS L , Cubilla Antonio , Mamede Marcelo 
Published: July 2023

Abstract
Introduction: Penile cancer (PC) is a rare tumor. It is associated with chronic inflammation and human papilloma virus (HPV) infection. Recent results of 18F-FDG PET/CT studies have demonstrated the great potential of 18F-FDG PET/CT as a noninvasive tool for assessing PC patients. Cancer cells overexpress transmembrane glucose transporters (GLUTs) and hexokinase (HK) during times of high glucose metabolism. Ki-67 is a nuclear protein linked to DNA replication. In PC, the HPV virus has oncoproteins that can interfere with the activities of the p16 and p53 tumor suppressor proteins associated with DNA replication, leading to changes related to PC carcinogenesis.
Purpose: Determine the correlation of the immunohistochemistry positivity of tumor molecular markers related to glucose metabolism, proliferation, and HPV with 18F-FDG uptake in penile cancer.
Procedures: Fifty-one patients were selected and underwent 18F-FDG PET/CT-based staging. Semiquantitative analysis was performed using the maximum standardized uptake value (SUVmax) and volumetric SUV (SUV2SD). HPV DNA was extracted and identified. Three pathologists performed immunohistochemistry analysis with anti-GLUT-1, anti-hexokinase-II, anti-Ki67, anti-p16, and anti-p53 antibodies.
Results: Of the 51 analyzed patients, 80.6% had invasive usual squamous cell carcinoma (SCC), and 42 patients were HPV+ (52.4%). Warty SCC showed the highest SUV value, and there were significant differences in SUVmax (p=0.015). Higher SUVmax and SUV2SD values were observed in grade 3 tumors. In typical invasive SCC, grade 3, HPV+, p16-negative, p53-negative, GLUT-1 i-3, and HK-II i-3 tumors showed a higher mean SUV. The Ki-67 value was significantly different for grade 3 tumors (p=0.001) and HK-II i-1 tumors (p=0.036). Ki-67 positivity was also higher in HPV-, p16 i-2, p53 i-3, and GLUT-1 i-3 tumors; none of the differences were statistically significant.
Conclusions: In PC, high uptake of 18F-FDG correlated with higher expression of glycolytic metabolism markers. There was a trend for positive correlation between increased expression of glucose transport markers and higher histological grade and higher Ki-67 expression. HPV-negative patients showed more significant relationships between PET/CT results and markers of proliferation.
The feedback loop of EFTUD2/c-MYC impedes chemotherapeutic efficacy by enhancing EFTUD2 transcription and stabilizing c-MYC protein in colorectal cancer
Authors: Zhu Xiaojian , Li Changxue , Gao Yufei , Zhou Huaixiang , Wang Tao , Bu Fanqin , Chen Jia , He Yulong , Wu Kaiming , Li Ningning , Luo Hongliang 
Published: July 2023

Abstract
Background
Chemoresistance remains the primary cause of mortality in colorectal cancer (CRC). However, our understanding of the molecular basis underlying CRC chemoresistance is still limited. Elongation factor Tu GTP binding domain containing 2 (EFTUD2) has been implicated as a potential oncogenic factor in various cancer types, yet its specific role in regulating the sensitivity of CRC cells to chemotherapy remains unclear.
Methods
Public datasets analysis, along with our in-house sample validation were conducted to identify the expression of EFTUD2 in 5-FU-resistant CRC cells and patients. Experiments both in vitro including MTT assay, EdU cell proliferation assay and clone formation assay and in vivo were performed to elucidate the function of EFTUD2 in sensitivity of CRC cells to 5-FU treatment. The underlying mechanism was investigated through molecular docking, chromatin immunoprecipitation (Ch-IP), dual luciferase reporter gene assay, and co-immunoprecipitation (Co-IP).
Results
We observed a positive correlation between increased expression of EFTUD2 and resistance to the chemotherapeutic agent 5-FU in CRC cells, as well as with higher pathological grades and poor prognosis. Then we showed that down-regulation of EFTUD2 expression enhanced the sensitivity of CRC cells to 5-FU treatment. Mechanistically, we uncovered that EFTUD2 interacted with and stabilized the oncoprotein c-MYC by preventing ubiquitin-mediated proteasomal degradation. Intriguingly, we found that c-MYC, acting as a transcription factor, directly activated the transcriptional expression of EFTUD2 by binding to its promoter region. Furthermore, rescue experiments demonstrated that the impact of EFTUD2 on reducing the efficacy of 5-FU chemotherapy relied on c-MYC stabilization.
Conclusion
Our findings revealed a positive feedback loop involving the EFTUD2/c-MYC axis that hampers the chemotherapeutic sensitivity of CRC cells to 5-FU chemotherapy, thereby impairing treatment effectiveness and fostering CRC progression. This study highlights EFTUD2 as a promising therapeutic target for overcoming chemotherapy resistance in CRC.
Thermographic evaluation of gingival phenotypes: correlation between morphological and thermodynamic parameters
Authors: de Melo Niebla Bezerra , Duarte Lígia Natália Sobreira , Pereira Camila Maia Vieira , Barbosa Jussara Silva , Silva Ariane Matos Gonçalves da , Soares Renata de Souza Coelho , Bento Patrícia Meira 
Published: July 2023

Abstract

Objectives
to evaluate the clinical and thermographic aspects of the gingival phenotype (GP) in healthy subjects.
Materials and methods
the study sample examined 264 upper and lower incisors, comprising 132 central incisors (CI) and 132 lateral incisors (LI). Four periodontal parameters were recorded systematically: ratio of width to length of the dental crown (CW/CL), height of the gingival attachment (GH), probing depth (PD) and gingival transparency (GT). The temperatures of the attached gingiva (GH Temp) and the free gingival margin (FGM Temp) were also recorded by way of infrared thermography (IRT).
Results
the average age of the sample was 30.70 ± 7.65 years. Of the 264 teeth evaluated, 76.1% had a thin GP. There was a significant association between CW/CL (p < 0.001), GH (p < 0.001), PD (p < 0.007) and FGM Temp (p < 0.006) with the tooth groups. The results show a significant and inversely proportional correlation between clinical parameters and gingival temperature (p < 0.05). A significant association was found between CW/CL (p < 0.026); GH (p < 0.001), and GP.
Conclusion
CW/CL and GH are determining parameters for GP. Moreover, an inversely proportional correlation can be observed between gingival temperature and the clinical morphological parameters that determine the GP.
Clinical Relevance:
The relationship between the clinical characteristics of the GPs and temperature could serve as one more parameter to help with the classification of gingival tissue. Moreover, our findings will help future studies evaluate the use of IR as an auxiliary diagnostic method in dentistry.
Impact of social protection on multidimensional poverty in Togo
Authors: DJAHINI-AFAWOUBO Dossè Mawussi , EVLO Kodjo , DJAHINI Afawoubo Komi Edem 
Published: July 2023

Abstract
This article first estimates the multidimensional poverty index using data from the Harmonized Survey of Household Living Conditions, conducted in 2018–2019 in Togo. Then, the paper examines the impact of social protection on multidimensional poverty. To account for selection biases, this paper employs the conditional mixed process, built from the seemingly unrelated regression framework. The findings show significant inequalities in the multidimensional poverty rate depending on the geographical zone, the education of the head of household, age, and the region. In particular, the level of multidimensional poverty is more than five times higher in rural areas than in urban areas. Using a variety of social protection indicators, the results clearly show that social protection significantly and statistically reduces the likelihood of being multi-dimensionally poor. The results also suggest that rural residents and economically disadvantaged regions are statistically and significantly more likely to be poor. There is no statistically significant impact of gender on multidimensional poverty.
Comparison of Procedural Anxiety and Pain Associated with Conventional Transrectal Ultrasound Prostate Biopsy to Magnetic Resonance Imaging-Ultrasound Fusion-guided Biopsy: A Prospective Cohort Trial
Authors: Deivasigamani Sriram , Adams Eric S , Kotamarti Srinath , Mottaghi Mahdi , Taha Tarek , Aminsharifi Alireza , Michael Zoe , Polascik Thomas 
Published: July 2023

Abstract
Background:
Prostate cancer (PCa) diagnosis relies on biopsy procedures, and the transrectal ultrasound biopsy remains widely used. However, fusion biopsy shows promise in improving diagnostic accuracy, but the pain and anxiety experienced by patients during biopsies is often overlooked. This study aims to compare pain and anxiety levels between MRI/US fusion biopsy (STB+FB) and standard TRUS-guided biopsy (STB).
Materials and Methods:
This IRB approved prospective trial, includes adult men undergoing prostate biopsy. Patients received identical peri-procedural care, including application of lidocaine jelly (2%) into the rectum and subsequent 1% lidocaine injections (10cc per side) into the prostate-seminal vesicle junction and prostatic apical areas bilaterally and the biopsy technique was chosen based on clinical indication and imaging findings. Patient anxiety levels were assessed using the validated STAI questionnaire, and post-biopsy pain using a numerical rating scale.
Results:
A total of 165 patients were included, with 99 undergoing STB and 66 undergoing STB+FB. There were no significant differences between the groups in terms of age, race, PSA level, prostate volume, and number of prior biopsies. The STB+FB group had a significantly higher number of biopsy cores taken (16.2 vs. 12 cores, p=0.001) and longer procedure time compared to STB (23 vs. 10 minutes, p=0.001). STB biopsy patients were found to experience lower post-procedural anxiety compared to STB+FB, a mean difference of -7 (p=0.001) and 89% of STB+FB group patients experienced severe post-procedural anxiety compared to 59% STB, p=0.002. No difference in pain (p=0.7) was found between the cohorts. The number of prior biopsies was associated with the severe post-procedural anxiety in the STB+FB group (p=0.04).
Conclusion:
MRI-targeted fusion plus systematic biopsy is associated with higher post-procedural anxiety compared to standard TRUS biopsy and patients with a prior history of repeat biopsies are more prone to experiencing severe anxiety.
Performance of qualitative and quantitative models in creating landslide susceptibility map of Chaliyar River basin
Authors: Aiswarya L , Rema K.P , Asha Joseph 
Published: July 2023

Abstract
A Landslide Susceptibility Map (LSM) for Kerala's Chaliyar river basin is what this study aims to provide. Several landslides occurred in this basin as a result of the floods in 2018 and 2019. There were 592 identified landslides. Using ArcGIS 10.7 software, the landslide inventory was taken from the inventories created by the NRSC and KSDMA, and the future incidence of landslides was projected by linking the landslide cause variables. Landslide inventories were split into training and validation groups in this study, with the ratios fixed at 70:30. For assessing landslide susceptibility, two models—a qualitative one called Weighted Linear Combination (WLC) and a quantitative one (a bivariate statistical model) called Weights of Evidence (WOE) model—were used. The following factors were employed as causative parameters: Slope, Aspect, Curvature, Relative Relief, TWI, Distance to Road, Distance to Streams, Distance to Lineaments, Land Use Land Cover, Drainage Density, Road Density, Lineament Density, Geomorphology, Soil Texture, and NDVI. The performance of the models was evaluated using the Receiver Operating Characteristic (ROCArea )'s. Under the Curve (AUC). In the study, the WLC model yields an AUC success rate accuracy of 59.9%, while the WOE model yields an accuracy of 70.9%. In terms of ratio of landslide validation accuracy, the WOE model outperforms the WLC model by 11%. The anticipated landslide area is included in the landslide susceptibility map, which can be incorporated to lessen the risk of landslides in this research area.
Green synthesis, characterization of copper nanoparticle synthesis from the leaf extract of Aegle marmelos and their biological activities
Authors: Pullapukuri Kalyani , Gopa Dinesh Reddy 
Published: July 2023

Abstract
Over the past few decades, the development of nanomaterial applications in numerous fields has been known to be significantly aided by green methods for the manufacture of metallic and metallic oxide nanoparticles. In comparison to several current physical and chemical approaches, the green production of copper and copper oxide nanoparticles (Cu and CuO, NPs) proved more practical and cost-effective. Applications for green-fabricated Cu and CuO Nanoparticles include energy, biomedicine, pharmaceutics, photocatalysis, electrocatalysis, organic dye degradation, and cosmetics. The bioactive elements of the plant extracts and the preparation circumstances have a significant impact on the shape and topology of biologically active Nanoparticles. The goal of the current review is to raise awareness of the numerous plants used in the biologically active manufacture of copper and its oxide nanoparticles (NPs) for use in catalysis and biological activity testing like Antimicrobial, antioxidant and anticancer. It has also been studied how to characterise biogenic copper nanoparticles for their chemical, morphological, and topographical properties using various analytical methods.