bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of preemptive middle glenohumeral ligament release in arthroscopic rotator cuff repair to reduce postoperative stiffness: A retrospective comparative study
Authors: Takahashi Ryosuke , Kajita Yukihiro , Harada Yohei , Iwahori Yusuke 
Published: February 2023

Abstract
Background: This study aimed to evaluate the efficacy of preemptive middle glenohumeral ligament (MGHL) release in arthroscopic rotator cuff repair (ARCR) to reduce postoperative stiffness.
Methods: Patients who underwent ARCR were enrolled and allocated into two groups retrospectively: the preemptive MGHL release group (n=44) and the preemptive MGHL non-release group (n=42). Clinical outcomes were assessed and compared between the two groups, including the range of motion, Japanese Orthopedic Association Shoulder Score, Constant Shoulder Score, and the University of California, Los Angeles Score preoperatively and 3 months, 6 months, and 12 months postoperatively and complications. The integrity of the repaired tendon was assessed at the 12-month follow-up using magnetic resonance imaging.
Results: There were no significant differences between the groups in all range of motion and all functional scores at any of the assessed time points. There was also no significant difference in the healing failure rate 2.3% in the preemptive MGHL group and 2.4% in the preemptive MGHL non-release group (p=.97), and postoperative stiffness was 2.3% in the preemptive MGHL group and 7.1% in the preemptive MGHL non-release group (p=.28). There was no postoperative instability in both group.
Conclusion: ARCR effectively facilitates the recovery of range of motion and function in patients with a rotator cuff tear. However, preemptive MGHL release could not be an effective method to reduce postoperative stiffness.
The coagulation-related genes for prognosis and tumor microenvironment in pancreatic ductal adenocarcinoma
Authors: Wang Di , Cui Song-ping , Chen Qing , Ren Zhang-yong , Lyu Shao-Cheng , Zhao Xin , Lang Ren 
Published: February 2023

Abstract
Background: Pancreatic ductal adenocarcinoma (PDAC) is the malignancy with difficult early diagnosis and poor prognosis. The coagulation is believed to affect tumor microenvironment of PDAC. The purpose of this study is to further identify the coagulation-related genes and explore immune infiltration in PDAC.
Methods: Two coagulation-related subtypes genes were collected in KEGG database. We obtained the transcriptome sequencing data and clinical information in PDAC from The Cancer Genome Atlas (TCGA) database. Patients were divided into different clusters using the unsupervised clustering method. We further explored the mutation frequency to study the genomic features. Enrichment analysis was performed and explored the pathways by Gene Ontology(GO) and Kyoto Encyclopedia of Genes(KEGG). The relationship between tumor immune infiltration and two clusters was analyzed by CIBERSORT. The prognostic model was constructed for risk stratification and the nomogram was established to facilitate the risk score. The response to immunotherapy was evaluated by IMvigor210 cohort. Finally, we recruit PDAC patients and collect the experiment samples to validate the Neutrophils infiltration using immunohistochemistry and identify the ITGA2 expression and function by Single cell sequencing analysis.
Results: Two coagulation-related clusters were divided based on the coagulation pathways in PDAC patients. Functional enrichment analysis showed different pathways in two clusters. The DNA mutation of coagulation-related genes occurred in about 49.4% of PDAC patients. Then the patients in two clusters had significant difference in immune cell infiltration, immune checkpoint, tumor microenvironment and TMB. We established the 4-genes prognostic stratified model through LASSO analysis. The nomogram based on risk score can accurately predict the prognosis in PDAC patients. We identified the hub gene ITGA2. ITGA2 was correlated with poor overall survival (OS) and short disease free survival (DFS). Single cell sequencing analysis showed that ITGA2 was expressed by Epithelial cells and high expression may enhance the function of tumor metastasis in PDAC.
Conclusions: We demonstrated the correlation the coagulation-related genes with tumor immune microenvironment, and the stratified model can predict the prognosis and calculate the drug therapy benefits, which provide the recommendation for clinical personalized treatment.
Valuation of provisioning ecosystem services and household dependency for livelihood: Empirical evidence from a tropical dry forest, India
Authors: Sharma Anil , Patel Sanoj Kumar , Singh Rinku , Tiwari Amit Kumar , Singh Gopal Shankar 
Published: February 2023

Abstract
The present study investigated the contribution of provisioning ecosystem services (PES) to the annual incomes of rural households in Vindhyan highlands, India. Household surveys, focus group discussions and key informant interviews were used to collect data, which was stratified by household size. A total of 225 households were chosen for further investigation from three villages adjacent to the forest. We employed one way ANOVA and Pearson’s correlation to analyze the quantitative data. The findings demonstrate that PES are critical in supplying rural livelihoods with fuelwood, fodder, leaves, wild edibles, medicines, oil seeds and resins. The overall value was estimated to be 24,781.66, 25,552.95, 16,494.46 INR per household per year for large size and 5467.39, 2671.12, 2029.62 INR per household per year for small size among the three villages. The results revealed that large households earn more than middle and small households in study area. The relationship between variables (household size, age, education, distance to forests and time spent) and PES income was tested using Pearson's correlation. The results exhibited that socio-economic factors impacted PES income substantially (p < 0.01, p < 0.05) in this study. The findings of this study can help policymakers to create mechanisms in conservation policies and regulations so that adjacent forest households can benefit from ecosystem provisions.
A simple model for pink noise from amplitude modulations
Authors: Morikawa Masahiro , Nakamichi Akika 
Published: February 2023

Abstract
We propose a simple model for the origin of pink noise (or 1/f fluctuation) based on the beat of cooperative waves. These cooperative waves arise spontaneously in a system with synchronization, resonance, and infrared divergence. Many cooperative waves with close frequencies can produce signals of arbitrary small frequencies from a system of small size. This beat mechanism can be understood as amplitude modulation. The pink noise can appear after the demodulation process, which produces a variety of pink noise in many fields. The pink noise thus formed from the beat has nothing to do with dissipation or long-time memory. We also suggest new ways of looking at pink noise in shallow earthquakes, solar flares, and stellar activities.
Outcomes of Neurorehabilitation Amongst Patients with Prolonged Disorders of Consciousness
Authors: Mohammed Mohammed Saif , Sharbatti Shatha Al , Nemmar Anas , Kumar Sharanya Suresh , Sharma Krishna Prasad , Banu Sharmila , Khan Asma Misal , Mahmoud Iman 
Published: February 2023

Abstract
Background: Less is known about the impact of Neurorehabilitation on patients with Prolonged Disorder of Consciousness. We assessed the Range of Motion, Muscle Girth and Power, Level of Consciousness, Development of Deformity, and Superficial Sensation.
Methods: A record-based study was done, including the data of patients diagnosed with prolonged disorder of consciousness who were admitted at the Long-term Rehabilitation Center-Thumbay Hospital, Ajman UAE, between 2020-2022. Data on the “Range of Motion”, “Muscle Girth and Power”, “Level of Consciousness”, “Development of Deformity”, and “Superficial Sensation” were collected and analysed. SPSS software version 27 was used for analysis. The Chi-square test was used to assess association, and the t-test was used to test the mean difference.
Results: We assessed the data of 21 patients with a prolonged disorder of consciousness. The Superficial sensation had a significant increment (p<0.025). There was a decrease in the proportion of patients with deformities during the follow-up period. The Range of Motion, Muscle Girth, and Muscle Power were also preserved without significant deterioration. However, the level of consciousness measured by the Glasgow Coma Scale (GCS) showed no improvement.
Conclusions: Our research showed that neurorehabilitation has a significant impact on improving superficial sensation and in the prevention of the development of deformities. However, the mean level of consciousness remained the same. There was also no decrease in the Range of Motion. The Muscle girth and power both were preserved over the course of 2 years.
Ensemble learning paradigms for flow rate prediction boosting
Authors: Kouadio Laurent Kouao , Liu Jianxin , Kouamelan Serge Kouamelan , Liu Rong 
Published: February 2023

Abstract

In developing countries, climate change has considerably affected population welfare by increasing drinking water scarcity. Global organizations and governments have initiated many drinking water supply projects to fight against this issue. Most of these projects are led by geophysical companies in partnership with drilling ventures to locate drillings expected to give the recommended flow rate (FR). Known as cheap methods, electrical resistivity profiling (ERP) and vertical electrical sounding (VES) were the most preferred. Unfortunately, the project objective was not achieved due to numerous unsuccessful drillings, thereby creating a huge loss of investments. To reduce the repercussion of unsuccessful drillings, we introduced the ensemble machine learning (EML) paradigms composed of four base learners. The aim is to predict at least 80% of correct FR in the validation set before any drilling operations. Geo-electrical features were defined from the ERP and VES and combined with the collected boreholes data to compose the binary dataset ( FR ≤ 1m3/hrandFR>1m3/hr) for unproductive and productive boreholes respectively). Then, the dataset is transformed before feeding to the EMLs. As a result, the benchmark and the pasting EMLs performed 85% of good predictions on the validation set whereas the extreme gradient boosting and the stacking performed 86% and 87% respectively. Finally, the correct prediction of FRs will reduce the losses in investment beneficial for funders and state governments, and geophysical and drilling ventures.
Assessment of Health Risks Associated with Contaminants in Groundwater in the Catchment Area of Selected Dumpsites in Abuja, North Central Nigeria
Authors: Omali Aurelius , arogundade johnson , Snow Daniel 
Published: February 2023

Abstract
The human health risk assessment associated with heavy metals contained in sources of drinking water within the catchment area of some dumpsites in Federal Capital Territory Abuja were carried out. Surface and groundwater samples obtained from the study area were subjected to analysis by inductively coupled plasma mass spectrometry (ICP-MS) in order to obtain the required analytes for the assessment. Health Risk Index (HI) was computed for non-carcinogenic risk assessment while carcinogenic Risk Index (CR) was computed for carcinogenic risk assessment. The computed HI for adults via ingestion across all the investigated dumpsites, ranges from 2.38 to 11.6. The computed HI for adults via dermal absorption across all the investigated dumpsites, ranges from 0.026 to 0.07, which is interpreted to be acceptable. The computed HI for children via ingestion across all the investigated dumpsites, ranges from 8.051 to 29.868, which is interpreted to be unacceptable. The computed HI for Children via dermal absorption across all the investigated dumpsites, ranges from 0.3820 to 1.237 in which about 80% are considered acceptable. The CR ranges from 0.0021 to 0.0109 and 0.004940 to 0.026 for adults and children respectively. This result indicates that both children and adults taking the investigated water via ingestion, are at high risk of contracting cancer.
An extract from the frass of swallowtail butterfly (Papilio machaon) larvae inhibits HCT116 colon cancer cell proliferation but not other cancer cell types
Authors: Nakano Miho , Sakamoto Takuma , Kitano Yoshikazu , Bono Hidemasa , Simpson Richard J. , Tabunoki Hiroko 
Published: February 2023

Abstract
Background
The frass of several herbivorous insect species has been utilised as natural medicines in Asia; however, the metabolite makeup and pharmaceutical activities of insect frass have yet to be investigated. Oligophagous Papilionidae insects utilise specific kinds of plants, and it has been suggested that the biochemicals from the plants may be metabolised by cytochrome P450 (CYP) in Papilionidae insects. In this study, we extracted the components of the frass of Papilio machaon larvae reared on Angelica keiskei, Oenanthe javanica or Foeniculum vulgare and examined the biological activity of each component. Then, we explored the expression of CYP genes in the midgut of P. machaon larvae and predicted the characteristics of their metabolic system.
Results
The components that were extracted using hexane, chloroform or methanol were biochemically different between larval frass and the host plants on which the larvae had fed. Furthermore, a fraction obtained from the chloroform extract from frass of A. keiskei-fed larvae specifically inhibited the cell proliferation of the human colon cancer cell line HCT116, whereas fractions obtained from the chloroform extracts of O. javanica- or F. vulgare-fed larval frass did not affect HCT116 cell viability. The metabolites from the chloroform extract from frass of A. keiskei-fed larvae prevented cell proliferation and induced apoptosis in HCT116 cells. Next, we examined the metabolic system in A. keiskei-fed larvae by RNA-seq analysis and found that the A. keiskei-fed larval midgut had different characteristics from the O. javanica- or F. vulgare-fed larval metabolic systems. We found that the CYP6B2 transcript was highly expressed in the A. keiskei-fed larval midgut.
Conclusions
These findings indicate that P. machaon metabolites might be useful as pharmaceutical agents against human colon cancer subtypes. Importantly, our findings show that it might be possible to use insect metabolic enzymes for the chemical structural conversion of plant-derived compounds with complex structures.
Canis MitoSNP Database: a Functional Tool Useful for Comparative Analyses of Human and Canine Mitochondrial Genomes
Authors: Kowal Krzysztof , Tkaczyk-Wlizło Angelika , Jusiak Marcin , Grzybowska-Szatkowska Ludmiła , Slaska Brygida 
Published: February 2023

Abstract
Canis MitoSNP is a tool allowing assignment of each mitochondrial genomic position a corresponding position in the mitochondrial gene and in the structure of tRNA, rRNA, and protein. The main aim of this bioinformatic tool was to use data from other bioinformatic tools (TMHMM, SOPMA, trna-scan, RNAfold, ConSurf) for dog and human mitochondrial genes in order to shorten the time necessary for the analysis of the whole genome single nucleotide polymorphism (SNP) as well as amino acid and protein analyses. Each position in the canine mitochondrial genome is assigned a position in genes, in codons, an amino acid position in proteins, or a position in tRNA or rRNA molecules. Therefore, a user analysing changes in the canine and human mitochondrial genome does not need to extract the sequences of individual genes from the mitochondrial genome for analysis and there is no need to rewrite them into amino acid sequences to assess whether the change is synonymous or nonsynonymous. Canis mitoSNP allows the comparison between the human and canine mitochondrial genomes as well. The Clustal W alignment of the dog and human mitochondrial DNA reference sequences for each gene obtained from GenBank (NC_002008.4 dog, NC_012920.1 human) was performed in order to determine which position in the canine mitochondrial genome corresponds to the position in the human mitochondrial genome. This function may be useful for the comparative analyses. The tool is available at: https://canismitosnp.pl.
Identification of key genes and lncRNAs based on M6A methylation regulators and Ferroptosis related genes for the slow flow/no reflow phenomenon
Authors: Zhang Chaoyue , Hu Zhao , Yang Ruijun , Shi Yunke , Ma Yiming , Cao Xingyu , Luo Xiang , Wang Jincheng , Lin Fanru , Li Xianbin , Lun Jinping , Tao Jifa , Cai Hongyan 
Published: February 2023

Abstract
Differentially expressed lncRNAs (DELncs) between treat (STEMI-A and STEMI-B groups) and control group and between STEMI-A (used as control group) and STEMI-B group were obtained by differential analysis. 29 common DELncs were obtained using Venn diagram by intersecting the two DELncs sets. Subsequently, four key lncRNAs based on the treat and control group (ABC-KLncs) with the highest correlation degree (|cor| > 0.8 and p < 0.05) with MMRs and FRGs were obtained, including FAM230B, lnc-EVI2A-3, lnc-PZP-6, and lnc-PLGLB2-5. Through differential analysis and correlation analysis, and 544 DEGs between the treat and control groups with |cor| > 0.3 were identified, and a PPI network was further constructed. MCODE algrithm and Cytohubba algrithm were applied and sifted out four key genes based on the treat and control group (ABC-KGs), consisting of ATP2B3, SUMO2, PPIA, and DNM1. Differential analysis, correlation analysis, PPI network, MCODE algrithm, and Cytohubba algrithm were also performed on the STEMI-A and STEMI-B groups. Moreover, four 4 key lncRNAs based on STEMI-A and STEMI-B groups (AB-KLncs) consisting of LINC00907, lnc-ZNF583-4, RP11-566J3.4, and lnc-USP28-6, and three key genes based the STEMI-A and STEMI-B groups (AB-KGs) consisting of TXN, ASPM, and MYLK were filtered out. In conclusion, this study obtained four ABC-KLncs, four ABC-KGs, four AB-KLncs, and three AB-KGs, these can inform the diagnosis and further study of STEMI as well as the phenomenon of slow flow/no reflow.
New high-throughput screening method for Chinese hamster ovary cell lines expressing low reduced monoclonal antibody levels: Application of a system controlling the gas phase over cell lysates in miniature bioreactors and facilitating multiple sample setup
Authors: Yamaguchi Tsuyoshi , Fukuda Mie , Matsumoto Yuichi , Mori Takaaki , Kikuchi Shinsuke , Nagano Ryuma , Yamamoto Koichi , Wakamatsu Kaori 
Published: February 2023

Abstract
Interchain disulfide bonds in monoclonal antibodies (mAbs) may be reduced during large-scale mAb production using Chinese hamster ovary (CHO) cells. This reaction lowers the mAb product yield and purity; however, it may be prevented by screening cell lines that are unsusceptible to reduction and using them in mAb production. Antibody reduction susceptibility may be cell line-dependent. To the best of our knowledge, however, an efficient method of screening reduction-unsusceptible CHO cell lines has not been previously reported. Here, we report a novel screening method that can simultaneously detect and identify mAb reduction susceptibility in lysates containing ≤ 48 CHO cell lines. This evaluation system was equally effective and generated similar results at all culture scales, including 250 mL, 3 L, and 1,000 L. Furthermore, we discovered that reduction-susceptible cell lines contained higher total intracellular nicotinamide adenine dinucleotide phosphate (NADPH) and NADP+ concentrations than reduction-unsusceptible cell lines, regardless of whether they expressed immunoglobulin (Ig)G4 or IgG1. NADPH or NADP+ supplementation in the lysate of reduction-unsusceptible cells resulted in mAb reduction. Application of the innovative CHO cell line screening approach could mitigate or prevent reductions in large-scale mAb generation from CHO cells.
Rational Design of Disulfide Bonds Increases Thermostability of a β-1,3-1,4-Glucanase from Paenibacillus
Authors: Wang Guo-Dong , Lu Jie , liang Xiao-Li , Cui Quan , Fan Han , Wang Rui-Ming , Li Pi-Wu , Wang Jun-Qing 
Published: February 2023

Abstract
β-1,3 − 1,4-gluconases can specifically hydrolyze the adjacent β-1,4 glycoside bond of β-1,3 in β-glucan, which is widely used in food, brewing and feed industries. Its sources include bacteria, fungi, and plant endosperm cell walls, most β-1,3 − 1,4-glucanases lose their activity when the temperature exceeds 65 ℃. In this study, we selected and modified the β-1,3 − 1,4-glucanase (PlicA) gene from Paenibacillus and expressed it in Escherichia coli BL21 (DE3). Adding disulfide bonds by rational design increased the optimal temperature of the enzyme from 55 ℃ to 80 ℃, and temperature stability was also improved. The optimum pH of the modified β-1,3 − 1,4-gluconanase (Eccsl69) was 9.0–10.0. The enzyme activity in 16.9 U/mL of Eccsl69 was measured at 540 nm with 0.8% gluan as the substrate, and a nickel column purified specific enzyme activity of 320 U/mg was determined. The Km and Vmax values of Eccsl69 using barley β-glucan as substrate were 1.5 mg/ml and 8.3 mol/min·mg. The structure of the β-1,3 − 1,4-glucanase Eccsl69 tended to be stable after molecular dynamics simulation for approximately 20 ns. The enzyme was successfully applied in the pulping and papermaking field for the first time, and the pulp freeness was adjusted from 55.0 °SR to 47 °SR, which enhanced water filtration. This study provides a successful strategy for improving the heat resistance of Eccsl69, which is promising for its application in pulping and paper making industries.
Experimental study of MICP low pH mixed grout curing sand
Authors: Liu Xiaojun , Zhang Zhao , Feng Zhongshuai , Zhu Rui 
Published: February 2023

Abstract
In this paper, the bacterial solution was mixed with cementing solution and the generation time of calcium carbonate precipitation was delayed during microbial curing. The optimal pH value and the concentration of cementing solution were determined. The reinforcement effect of pH-adjusted mixed grouting method on sandy soil was analyzed by the unconfined compressive strength, the generation amount and distribution of calcium carbonate precipitation. The results show that the strength of the specimen by low pH mixed grouting method is 235% higher and more calcium carbonate precipitates is generated than other. The utilization rate of cementing fluid and the distribution of calcium carbonate are better.
Osteocyte-derived exosomes confer multiple myeloma resistance to chemotherapy through acquisition of cancer stem cell-like features
Authors: Cheng Feifei , Wang Zhiming , You Gichun , Liu Yuhong , He Jin , Yang Jing 
Published: February 2023

Abstract
Therapeutic resistance is a major challenge in multiple myeloma treatment. Understanding the underlying mechanisms is required to develop effective strategies against drug resistance and improve the prognosis of myeloma patients. Here, we identify osteocytes, the major cellular component of bone tissue, as key regulators of myeloma therapeutic resistance. Osteocyte-derived exosomes can be efficiently taken up by myeloma cells and exert a protective effect against chemotherapy-induced apoptosis. Mechanistic studies further reveal that osteocyte-derived exosomal miR-483-3p and miR-513a-5p promote cancer stem cell-like features in myeloma cells by regulating HIF-1α stabilization, thus conferring myeloma cells resistance to chemotherapy. Strikingly, combination treatment of miR-483-3p and miR-513a-5p inhibitors significantly reduces tumor burden and potentiates the therapeutic efficacy of bortezomib in the myeloma mouse model. Our findings, therefore, demonstrate the functional impact of osteocytes on myeloma therapeutic resistance, and suggest that osteocyte-derived exosomal miRNAs may serve as potential therapeutic targets for overcoming drug resistance in multiple myeloma.