bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing agronomic performance of industrial hemp varieties for suitable production in the United States
Authors: Williams Ajit , Brym Zachary , Chen Chengci , Collins Alyssa , Crawford Jamie , Darby Heather , Dedecker James , Ellison Shelby , Fike John , Gage Karla , Gang David , Griffin Jason , Johnson Burton , Moore Virginia , Ortmeier‐Clarke Haleigh , Podder Swarup , Richmond Mitchell , Roozeboom Kraig , Thelen Kurt , Werle Rodrigo , Pearce Robert 
Published: January 2025

AbstractIndustrial hemp (Cannabis sativa L.) is an ancient crop used throughout history for fiber, oilseed, and therapeutic compounds. Hemp varieties were cultivated across diverse environments in the United States, but knowledge of those agronomic practices along with genetic resources was lost during a period in which cultivation of cannabis was prohibited. Therefore, regional performance evaluations of hemp varieties for crop performance coupled with scientific communication of outcomes to the public are crucial for hemp's development as an agricultural commodity. Objectives for this research were to evaluate relative yields of industrial hemp varieties grown across the United States and link their suitability for commercial production across locations. A national collaboration established variety trials containing seven industrial hemp varieties planted across 14 locations (36°–48° N latitude and 72°–110° W longitude) over a 3‐year period. Crop dry straw yield and seed yield increased from the averages of 1600 and 700 kg ha−1 in Year 1 to 2400 and 1150 kg ha−1 in Year 2, and 3050 and 815 kg ha−1 in Year 3, respectively. The varieties Anka and X‐59 performed best in Vermont and Virginia, where seed yields consistently exceeded 1100 kg ha−1; however, no single variety performed above average across all sites. Overall, this assessment identified two industrial hemp varieties suitable for commercial production in specific sites and highlighted the importance for hemp breeders to investigate variety × location × year interactions when developing improved varieties to best capture site‐specific productivity.
Plasma p‐tau217 and neurofilament/p‐tau217 ratio in differentiating Alzheimer's disease from syndromes associated with frontotemporal lobar degeneration
Authors: Benussi Alberto , Huber Hanna , Tan Kübra , Cantoni Valentina , Rivolta Jasmine , Cotelli Maria Sofia , Benedet Andrea L. , Blennow Kaj , Zetterberg Henrik , Ashton Nicholas J. , Borroni Barbara 
Published: January 2025

AbstractINTRODUCTIONPlasma‐based biomarkers have shown promise for clinical implementation, but their accuracy in differentiating Alzheimer's disease (AD) from syndromes associated with frontotemporal lobar degeneration (FTLD) has yet to be fully investigated. This study assessed the potential of plasma biomarkers for differential diagnosis.METHODSThis cohort study included 374 participants (96 AD, 278 FTLD). Plasma phosphorylated tau (p‐tau)217, neurofilament light chain (NfL), brain‐derived tau, glial fibrillary acidic protein, and the amyloid beta1‐42/1‐40 ratio were measured. Receiver operating characteristic curve analyses assessed diagnostic accuracy, and a three‐range threshold approach was used to stratify patients based on the most accurate biomarker.RESULTSPlasma p‐tau217 effectively distinguished AD from FTLD, with the NfL/p‐tau217 ratio showing superior accuracy. The three‐range approach identified thresholds with 95% and 97.5% sensitivity and specificity, reducing the need for cerebrospinal fluid testing by 75% and 54%, respectively.DISCUSSIONPlasma p‐tau217 and the NfL/p‐tau217 ratio are promising non‐invasive biomarkers for differentiating AD from FTLD, suggesting their use as a potential alternative to traditional diagnostic methods.Highlights
Plasma phosphorylated tau (p‐tau)217 distinguishes Alzheimer's disease (AD) from frontotemporal lobar degeneration (FTLD) with high accuracy.
The neurofilament light chain/p‐tau217 ratio showed the highest accuracy for differentiating AD from FTLD.
A three‐range threshold reduces the need for invasive cerebrospinal fluid testing or amyloid positron emission tomography imaging.
Pathological angiogenesis was associated with cerebrovascular lesion and neurodegeneration in Alzheimer's disease
Authors: Gao Feng , Zhan Yaxi , Wang Qiong , Zhang Mengguo , Dai Linbin , Shen Yong 
Published: January 2025

AbstractINTRODUCTIONWe investigated the specific factors driving abnormal angiogenesis in Alzheimer's disease (AD) and its role in cerebrovascular lesions and neurodegeneration.METHODSWe assessed cerebrovascular pathologies, amyloid‐beta (Aβ), and tau pathologies in post mortem human brains and detected 12 angiogenic factors in cerebrospinal fluid (CSF) from the China Aging and Neurodegenerative Disease Initiative (CANDI) cohort.RESULTSWe observed severe blood‐brain barrier damage and elevated levels of the vascular marker CD31 in human AD brains, which had a stronger correlation with tau pathology than Aβ pathology. Consistently, only CSF pTau181 showed positive associations with CSF angiogenesis factors (soluble vascular endothelial growth factor receptor 2 [sVEGFR2], VEGF‐C, VEGF‐D, placental growth factor [PLGF], Angiopoietin2, and Serpin E1), but not CSF Aβ42/40. Additionally, higher levels of CSF sVEGFR1, soluble Tyrosine‐protein kinase receptor 2 [sTIE2], PLGF, and interleukin 8 [IL8], as well as lower levels of CSF urokinase‐type plasminogen activator [uPA], were associated with worsen cerebrovascular pathologies and neurodegeneration.DISCUSSIONOur findings indicate that tau pathology may play a critical role in pathological angiogenesis, contributing to cerebrovascular lesions and neurodegeneration in AD.HIGHLIGHTS
BBB damage and elevated vascular marker CD31 in human AD brains had a stronger correlation with tau pathology than Aβ pathology.
CSF pTau181 mediated the effect of CSF Aβ42/40 on CSF sVEGFR1 and sTIE2.
Only CSF pTau181 showed positive associations with sVEGFR2, VEGF‐C, VEGF‐D, PLGF, Angiopoietin2, and Serpin E1.
Higher sVEGFR1, sTIE2, PLGF, and IL8, and lower uPA in CSF, were associated with cerebrovascular pathologies and neurodegeneration.
Active Hydroxyl‐Mediated Preferential Cleavage of Carbon‐Carbon Bonds in Electrocatalytic Glycerol Oxidation
Authors: Zhang Qiang , Zhang Xiaojing , Liu Baocang , Jing Peng , Xu Xuan , Hao Haigang , Gao Rui , Zhang Jun 
Published: January 2025

AbstractElectrocatalytic glycerol oxidation reaction (GOR) to produce high‐value formic acid (FA) is hindered by high formation potential of active species and sluggish C−C bond cleavage kinetics. Herein, Ni single‐atom (NiSA) and Co single‐atom (CoSA) dual sites anchored on nitrogen‐doped carbon nanotubes embedded with Ni0.1Co0.9 alloy (Ni0.1Co0.9@NiSACoSA‐NCNTs) are constructed for electrochemical GOR. Remarkably, it can reach 10 mA cm−2 at a low potential of 1.15 V versus the reversible hydrogen electrode (vs. RHE) and realize a high formate selectivity of 93.27 % even at high glycerol conversion of 98.81 % at 1.45 V vs. RHE. The GOR mechanism and pathway are systematically elucidated via experimental analyses and theoretical calculations. It is revealed that the active hydroxyl (*OH) can be produced during the GOR. The NiSA, CoSA, and Ni0.1Co0.9 synergistically optimizes the electronic structure of CoSA active sites, reducing the energy barriers of *OH‐mediated cleavage of C−C bonds and dehydrogenation of C1 intermediates. This decreases the number of reaction intermediates and reaction steps of GOR‐to‐FA, thus increasing the formate production efficiency. After coupling GOR with hydrogen evolution reaction in a membrane electrode assembly cell, 14.26 g of formate and 23.10 L of H2 are produced at 100 mA cm−2 for 108 h.
B,N‐Embedded Helical Nanographenes Showing an Ion‐Triggered Chiroptical Switching Function
Authors: Maeda Chihiro , Michishita Sayaka , Yasutomo Issa , Ema Tadashi 
Published: January 2025

AbstractIntramolecular oxidative aromatic coupling of 3,6‐bis(m‐terphenyl‐2’‐yl)carbazole provided a bis(m‐terphenyl)‐fused carbazole, while that of 3,6‐bis(m‐terphenyl‐2’‐yl)‐1,8‐diphenylcarbazole afforded a bis(quaterphenyl)‐fused carbazole. Borylation of the latter furnished a B,N‐embedded helical nanographene binding a fluoride anion via a structural change from the three‐coordinate boron to the four‐coordinate boron. The anionic charge derived from the fluoride anion is stabilized over the expanded π‐framework, which leads to the high binding constant (Ka) of 1×105 M−1. The four‐coordinate boron species was converted back to the parent three‐coordinate boron species with Ag+, and the chiroptical switch between the three‐coordinate boron and four‐coordinate boron species has been achieved via the ion recognition with the change in the color and glum values.
Cell‐Active, Arginine‐Targeting Irreversible Covalent Inhibitors for Non‐Kinases and Kinases
Authors: Chen Peng , Wang Lu , Wang Xuan , Sun Jie , Miao Fengfei , Wang Zuqin , Yang Fang , Xiang Menghua , Gu Mingxi , Li Shengrong , Zhang Jianzhong , Yuan Peiyan , Lu Xiaoyun , Zhang Zhi‐Min , Gao Liqian , Yao Shao Q. 
Published: January 2025

AbstractTargeted covalent inhibitors (TCIs) play an essential role in the fields of kinase research and drug discovery. TCI strategies to target more common amino acid side‐chains have yet to be demonstrated. Targeting other amino acids would also expand the pharmaceutical industry‘s toolbox for targeting other tough‐to‐drug proteins. We report herein a glyoxal‐based, arginine‐reactive strategy to generate potent and selective small‐molecule TCIs of Mcl‐1 (an important anti‐apoptotic protein) by selectively targeting the conserved arginine (R263) in the protein. We further validated the generality of this strategy by developing glyoxal‐based, irreversible covalent inhibitors of AURKA (a cancer‐related kinase) that showed exclusive reactivity with a solvent‐exposed arginine (R220) of this enzyme. We showed the resulting compounds were potent, selective and cell‐active, capable of covalently engaging endogenous AURKA in MV‐4–11 cells with long residence time. Finally, we showed the potential application of glyoxal‐based TCIs in targeting an acquired drug‐resistance mutant of ALK kinase (G1202R).
Diorganotin(IV) Complexes Containing Hydrazone Ligands as Prospective Bioactive Agents: Synthesis, Crystal Structure, Spectroscopic Analysis, Anticancer, and Antimicrobial Activity
Authors: Barwa Pinki , Asija Sonika , Deswal Yogesh , Kumar Deepak , Jindal Deepak Kumar , Ghosh Sandip , Basu Biswarup , Poonia Shikha , Ragshaniya Anju 
Published: January 2025

ABSTRACTThe exploration of diorganotin(IV) compounds as potential inhibitors of cancer cell growth is gaining attention. Here, we have emphasized the potential of diorganotin derivatives as promising candidates for anticancer therapy. Thus, a series of Schiff base core tin(IV) complexes, including dimethyl, diethyl, dibutyl, and diphenyl derivatives, were synthesized and examined utilizing mass spectrometry, FT‐IR, NMR, and single crystal XRD. The acquired spectroscopy results disclosed that hydrazone ligands connected to tin atom in a tridentate fashion via phenolic O, imine N, and enolic O donor atoms and formed a pentacoordinated environment, which was further validated by X‐ray crystallographic diffraction study of compound 9. Single crystal X‐ray examination of compound 9 demonstrated that the Sn atom is arranged in a distorted five‐coordinated square pyramidal geometry. The antimicrobial potential of all prepared compounds was examined against four bacterial and two fungal strains, and complexes 7, 11, and 15 having MIC values between 0.0043 and 0.0050 μmol/mL exhibit better effectiveness against Escherichia coli and Candida albicans. Further, synthesized compounds were examined for in vitro anticancer efficacy against MCF‐7 and A549 cell lines and demonstrated substantial activity with standard Doxorubicin. Compounds 3 and 5, being the most active, were subsequently evaluated against normal cell line HEK 293T.
Virus Filtration Development for Adeno‐Associated Virus‐Based Gene Therapy Products
Authors: Namila FNU , Zhou Tianyi , Wang Lu , Jin Mi 
Published: January 2025

ABSTRACTAdeno‐associated virus (AAV) vectors have become a leading platform for gene delivery. A major portion of gene therapy currently in clinical trials are AAV‐based for a wide range of diseases. A commonly used method for AAV production is by mammalian or insect cell culture, with or without added viruses to introduce needed genetic elements for AAV production. There are potential risks of virus contamination from the production cell line, process residuals, or adventitious contamination in the production of biotherapeutics, including AAV‐based gene therapy products; therefore, it is imperative to demonstrate that the drug substance manufacturing process has sufficient capability to clear process‐related or adventitious viruses. In the AAV‐based gene therapy manufacturing process, cell lysis, affinity chromatography, and ion exchange chromatography steps are often effective to inactivate or remove viruses. To increase the viral clearance robustness, virus filtration (VF) is increasingly recommended by regulatory agencies for gene therapy products as a dedicated viral clearance step in the downstream purification process. In the current study, two commercially available virus filters were evaluated in the context of AAV manufacturing. The filter throughput and process yield were assessed under different operational modes. Virus clearance performance was evaluated by spiking in Adenovirus type 5 (Adv‐5) and Simian virus 40 (SV‐40). The viral filters assessed in this study demonstrated manufacturable throughputs, acceptable process yields, and robust virus clearance capabilities for viruses greater than 40 nm.
Validated HPLC‐UV Method for the Quantification of a Novel BCr‐Abl 1 Inhibitor, Vodobatinib, in Rat Plasma: Application to a Pharmacokinetic Study
Authors: Kaddare Sandeep , Zakkula Ashok , Bestha Rama Murthi , Madipelli Keerthana , Kumar Rajnish , Biradar Yogesh Balaji , Veerla Niranjan , Dittakavi Sreekanth , Mullangi Ramesh 
Published: January 2025

ABSTRACTVodobatinib is a Bcr‐Abl 1 inhibitor, currently entering into Phase 2 clinical trials as a potential drug to treat glioblastoma patients. In the present work, a validated high‐performance liquid chromatography (HPLC) detection method for the quantification of vodobatinib in rat plasma was established. Sample preparation involved liquid–liquid extraction method. The chromatographic separation of vodobatinib and the IS was accomplished on an XBridge C18 column using 10 mM ammonium acetate and acetonitrile in gradient condition. The flow‐rate and injection volume were 0.8 mL/min and 20 μL, respectively. The eluent was detected at 310 nm. Vodobatinib and the IS eluted at 7.5 and 5.9 min, respectively, with a total run time of 10 min. Validation tests were performed according to FDA requirements. The established method showed good linearity in the range of 50.5–6534 ng/mL. Interday and intraday coefficients of variations were < 5.11%. Stability studies, dilution integrity, and incurred sample reanalysis met the acceptance criteria. Subsequently, the validated HPLC method was used to study the disposition kinetics of vodobatinib in rats.
Clinical Outcomes According to the Extent of Atherosclerotic Disease in Female Patients Undergoing Transcatheter Aortic Valve Replacement: An Analysis From the WIN‐TAVI Registry
Authors: Bay Benjamin , Gitto Mauro , Sartori Samantha , Vogel Birgit , Tchetche Didier , Petronio Anna Sonia , Mehilli Julinda , Di Muro Francesca Maria , Lefevre Thierry , Presbitero Patrizia , Capranzano Piera , Oliva Angelo , Iadanza Alessandro , Leone Pier Pasquale , Sardella Gennaro , van Mieghem Nicolas M. , Kim Chan Joon , Meliga Emanuele , Feng Yihan , Dumonteil Nicolas , Fraccaro Chiara , Trabattoni Daniela , Mikhail Ghada , Ferrer‐Gracia Maria‐Cruz , Naber Christoph , Sharma Samin K. , Watanabe Yusuke , Morice Marie‐Claude , Dangas George D. , Chieffo Alaide , Mehran Roxana 
Published: January 2025

ABSTRACTBackgroundLimited data exist on the impact of polyvascular disease (PolyVD) on clinical outcomes in female patients undergoing transcatheter aortic valve replacement (TAVR). We therefore sought to investigate clinical outcomes in women with versus without PolyVD undergoing TAVR.MethodsFemale participants from the multicentre Women's International Transcatheter Aortic Valve Implantation (WIN‐TAVI) registry were categorized based on the presence or absence of PolyVD. The PolyVD population was defined as the presence of atherosclerotic disease affecting ≥ 2 arterial systems from coronary, cerebral, or lower limb peripheral vessels, whilst patients with either no atherosclerosis or atherosclerotic disease in one vascular system were included in the non‐PolyVD population. The primary endpoint was the Valve Academic Research Consortium‐2 consensus (VARC‐2) efficacy endpoint at 1 year, whilst secondary endpoints included VARC‐2 safety events, VARC‐2 major bleeding and major vascular complications. Cox regression analysis were computed adjusting for various cofounders.ResultsAmong 996 participants, 543 (54.5%) had PolyVD, while 453 (45.5%) did not. Across the subgroups no differences in age was noted, whilst patients with PolyVD were more likely to have a history of hypercholesterolemia and a previous cardiac surgery. The incidence of the primary endpoint was higher in the PolyVD group (19.4%) compared to the non‐PolyVD group (13.3%, plog‐rank = 0.014), though the difference was attenuated after multivariable adjustments (p = 0.093). Of note, no statistically significant differences concerning incident VARC‐2 safety events, VARC‐2 major bleeding and major vascular complications were noted according to PolyVD status.ConclusionPolyVD is a common comorbidity and is associated with elevated rates of adverse clinical events, but no increase in safety events, vascular complications, or bleeding among women undergoing TAVR.
cHild ‐in‐mothEr Approach for DeliverING Renal Denervation System: The HEADING‐IN Technique
Authors: Viola Orazio , Verdoia Monica , Mitro Manuela , Rognoni Andrea 
Published: January 2025

ABSTRACTRenal denervation is an emerging strategy for the management of uncontrolled hypertension. However, real‐world experience is still modest, in particular for the management of complex anatomy, with available data being limited to the selected population of randomized clinical trials. We first describe the feasibility of delivering the renal denervation system to the target site with a child‐in‐mother technique, using a common coronary guiding extension, in a patient with severe tortuosity and double renal arteries.
Data Analysis Methods in Time‐Resolved Fluorescence Spectroscopy: A Tutorial Review
Authors: de Jong Flip , Martín Cristina , Hofkens Johan , Van der Auweraer Mark 
Published: January 2025

AbstractFluorescence spectroscopy and related techniques benefit from exceptional sensitivity and have become engrained in a variety of fields from biosciences to materials sciences. Measuring time‐domain fluorescence decays is nowadays a routine task in many laboratories across these different fields. Perhaps surprisingly, a correct data analysis of these fluorescence decay curves presents a formidable challenge and requires extensive insight in the problems associated with fitting this type of data. As a result, the reported analysis of these decays is usually limited to a non‐linear least squares fit of a sum of a few exponential terms to the data. This review aims to expose the intricate field of data analysis in time‐resolved fluorescence spectroscopy to a broader audience, from researchers interested in understanding the photophysics of their system to readers and reviewers trying to understand the merits of specific methods. Challenges associated with this type of kinetic experimental data are outlined and the clever analysis strategies devised by researchers across different disciplines are introduced and discussed in detail. A section on freely available scripts and software facilitating the analysis is included towards the end. We encourage the reader to try their hand at the worked examples.
Effect of Excitation Wavelength in Single‐Molecule Photochemistry of Terrylene
Authors: Mhanna Rana , Berger Julia , Jourdain Matthias , Muth Stephan , Kutta Roger Jan , Jung Gregor 
Published: January 2025

AbstractThe reaction of terrylene in p‐terphenyl with molecular oxygen is reinvestigated by TIRF‐microscopy with λexc=488 nm or λexc=561 nm and 488 nm. A similar range of fluorescent products is obtained under both experimental conditions with a reaction quantum yield Φr>10−7 for those molecules which undergo the photoreaction. The majority of these oxygen‐susceptible molecules reacts via an electronically relaxed, dark intermediate, presumably an endoperoxide, with a lifetime of <toff>~20 s. From this time constant, an activation energy EA<0.8 eV is estimated for the transition from the intermediate to the final product, the diepoxide, which nicely agrees with values calculated for the terrylene‐derivative TDI. However, ~20 % of all reacting molecules at λexc=561 nm and even ~40 % at λexc=488 nm show an immediate change of the fluorescence colour within the time resolution of the experiment, bypassing any dark intermediate. Based on this experimentally observed impact of the excitation energy and the lack of relevant excited‐state absorption, we hypothesize that oxygen forms a complex with ground‐state terrylene which then undergoes a quasi‐unimolecular reaction in the excited‐state before vibrational relaxation takes place.
Research Progress on Stability of FAPbI3 Perovskite Solar Cells
Authors: Deng Wenxin , Wei Jianwei , Ma Zengwei , Feng Wenlin 
Published: January 2025

AbstractThe formamidinium lead iodide (FAPbI3) perovskite has emerged as a promising material for high‐efficiency photovoltaic applications. Although a power conversion efficiency of more than 26% has been achieved, stability issues have hindered its commercial application. In this study, the stability of FAPbI3 under adverse conditions such as humidity, oxygen, ultraviolet light, and temperature fluctuations is systematically reviewed. The known effective strategies for improving stability are discussed. Current studies have shown that technologies such as doping, halide alloying, additive manufacturing engineering, and interface modification have been identified as effective in mitigating phase transitions of FAPbI3 and enhancing environmental durability. Encapsulation technology further improves moisture and heat resistance. Compared with other stabilization strategies, doping and alloying can address the adverse effects of narrowing of the absorption edge. Interface engineering has an essential understanding of the stability mechanism, which will greatly improve the stability problem in the practical application of FAPbI3. This paper also looks forward to the future research directions and development trends.