bluuebunny/crossref_metadata_2025_split huggingface dataset:
2021 superoutburst of the WZ Sge-type dwarf nova V627 Pegasi lacks an early superhump phase
Authors: Tampo Yusuke , Kato Taichi , Kojiguchi Naoto , Shugarov Sergey Yu , Itoh Hiroshi , Matsumoto Katsura , Nakagawa Momoka , Nishida Yukitaka , Richmond Michael , Shibata Masaaki , Ito Junpei , Kokhirova Gulchehra , Rakhmatullaeva Firuza , Tordai Tamás , Kiyota Seiichiro , Ruiz Javier , Dubovsky Pavol A , Medulka Tomáš , Pavlenko Elena P , Antonyuk Oksana I , Sosnovskij Aleksei A , Baklanov Aleksei V , Krushevska Viktoriia , Vanmunster Tonny , Brincat Stephen M , Petrik Karol , Galdies Charles , Hambsch Franz-Josef , Maeda Yutaka , Nogami Daisaku 
Published: April 2023

Abstract
Superoutbursts in WZ Sge-type dwarf novae (DNe) are characterized by both early superhumps and ordinary superhumps originating from the 2 : 1 and 3 : 1 resonances, respectively. However, some WZ Sge-type DNe show a superoutburst lacking early superhumps; it is not well established how these differ from superoutbursts with an early superhump phase. We report time-resolved photometric observations of the WZ Sge-type DN V627 Peg during its 2021 superoutburst. The detection of ordinary superhumps before the superoutburst peak highlights that this 2021 superoutburst of V627 Peg, like that in 2014, did not feature an early superhump phase. The duration of stage B superhumps was slightly longer in the 2010 superoutburst accompanied by early superhumps than that in the 2014 and 2021 superoutbursts, which lacked early superhumps. This result suggests that an accretion disk experiencing the 2 : 1 resonance may have a larger mass at the inner part of the disk and hence needs more time for the inner disk to become eccentric. The presence of a precursor outburst in the 2021 superoutburst suggests that the maximum disk radius should be smaller than that of the 2014 superoutburst, even though the duration of quiescence was longer than that before the 2021 superoutburst. This could be accomplished if the 2021 superoutburst was triggered as an inside-out outburst or if the mass transfer rate in quiescence changes by a factor of two, suggesting that the outburst mechanism and quiescence state of WZ Sge-type DNe may have more variety than ever thought.
Binding by the Polycomb complex component BMI1 and H2A monoubiquitination shape local and long-range interactions in the Arabidopsis genome
Authors: Yin Xiaochang , Romero-Campero Francisco J , Yang Minqi , Baile Fernando , Cao Yuxin , Shu Jiayue , Luo Lingxiao , Wang Dingyue , Sun Shang , Yan Peng , Gong Zhiyun , Mo Xiaorong , Qin Genji , Calonje Myriam , Zhou Yue 
Published: April 2023

Abstract
Three-dimensional (3D) chromatin organization is highly dynamic during development and seems to play a crucial role in regulating gene expression. Self-interacting domains, commonly called topologically associating domains (TADs) or compartment domains (CDs), have been proposed as the basic structural units of chromatin organization. Surprisingly, although these units have been found in several plant species, they escaped detection in Arabidopsis (Arabidopsis thaliana). Here, we show that the Arabidopsis genome is partitioned into contiguous CDs with different epigenetic features, which are required to maintain appropriate intra-CD and long-range interactions. Consistent with this notion, the histone-modifying Polycomb group machinery is involved in 3D chromatin organization. Yet, while it is clear that Polycomb repressive complex 2 (PRC2)-mediated trimethylation of histone H3 on lysine 27 (H3K27me3) helps establish local and long-range chromatin interactions in plants, the implications of PRC1-mediated histone H2A monoubiquitination on lysine 121 (H2AK121ub) are unclear. We found that PRC1, together with PRC2, maintains intra-CD interactions, but it also hinders the formation of H3K4me3-enriched local chromatin loops when acting independently of PRC2. Moreover, the loss of PRC1 or PRC2 activity differentially affects long-range chromatin interactions, and these 3D changes differentially affect gene expression. Our results suggest that H2AK121ub helps prevent the formation of transposable element/H3K27me1-rich long loops and serves as a docking point for H3K27me3 incorporation.
Accelerating synergistic effects of preconditioned mesenchymal stem cells with Crocin and dexamethasone in pulmonary epithelial cells injury
Authors: Jamshidi Vahid , Halabian Raheleh , Saeedi Pardis , Bagheri Hasan , Nobakht Motlagh Ghoochani Bibi Fatemeh 
Published: April 2023

Abstract
Chemical warfare victims suffer from bronchiolitis and chronic pulmonary obstruction caused by sulfur mustard (SM) toxicity. Despite the mesenchymal stem cells capacity to alleviate inflammation, their low survival rate under oxidative stress severely limits their effectiveness. This study aimed to examine how natural (Crocin) and synthetic (Dexamethasone) antioxidants might affect MSC efficacy. MSCs were treated with the optimal doses of Crocin (Cr.), Dexamethasone (Dex.), and their combination. The A549 cells line was pretreated with the optimal dose of the CEES to mimic the lung disease. Then, the affected A549 cells were exposed to the preconditioned MSCs and conditioned media, and then their survival rates were estimated by MTTor2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Annexin-V PI apoptosis test was conducted for MSCs and A549 cells. Reactive Oxygen Species (ROS) assay and Enzyme-linked immunosorbent assay (ELISA) test demonstrated the percentage of production of ROS and the cytokines levels in A549/CEES, respectively. The results revealed significant increases in Cr. + Dex. treated MSCs (P &lt; .01) and A549 cells treated with MSCs-CM/Cr/Dex (P &lt; .01) groups’ survival. The apoptosis rate and ROS production were reduced in the MSCs-CM/Cr/Dex. Also, considerable decreases in IL-1β (P &lt; .01) and IL-6 (P &lt; .01) and a significant increase in IL-10 (P &lt; .05) in treated A549/CEES by Cr/Dex and MSCs-CM/Cr/Dex supported the synergistic effects of Crocin and Dexamethasone.
Previsual and Early Detection of Myrtle Rust on Rose Apple Using Indices Derived from Thermal Imagery and Visible-to-Short-Infrared Spectroscopy
Authors: Watt Michael S. , Bartlett Michael , Soewarto Julia , de Silva Dilshan , Estarija Honey Jane C. , Massam Peter , Cajes David , Yorston Warren , Graevskaya Elizaveta , Dobbie Kiryn , Fraser Stuart , Dungey Heidi S. , Buddenbaum Henning 
Published: April 2023

Myrtle rust, caused by the fungus Austropuccinia psidii, is a serious disease, which affects many Myrtaceae species. Commercial nurseries that propagate Myrtaceae species are prone to myrtle rust and require a reliable method that allows previsual and early detection of the disease. This study uses time-series thermal imagery and visible-to-short-infrared spectroscopy measurements acquired over 10 days from 81 rose apple plants ( Syzygium jambos) that were either inoculated with myrtle rust or maintained disease-free. Using these data, the objectives were to (i) quantify the accuracy of models using thermal indices and narrowband hyperspectral indices (NBHI) for previsual and early detection of myrtle rust using data from older resistant green leaves and young susceptible red leaves and (ii) identify the most important NBHI and thermal indices for disease detection. Using predictions made on a validation dataset, models using indices derived from thermal imagery were able to perfectly (F1 score = 1.0; accuracy = 100%) distinguish control from infected plants previsually one day before symptoms appeared (1 DBS) and for all stages after early symptoms appeared. Compared with control plants, plants with myrtle rust had lower and more variable normalized canopy temperature, which was associated with higher stomatal conductance and transpiration. Using NBHI derived from green leaves, excellent previsual classification was achieved 3 DBS, 2 DBS, and 1 DBS (F1 score range = 0.89 to 0.94). The accurate characterization of myrtle rust during previsual and early stages of disease development suggests that a robust detection methodology could be developed within a nursery setting. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
A Draft Genome Resource for Marasmius tenuissimus, an Emerging Causal Agent of Thread Blight Disease in Cacao
Authors: Leung Jason , Cohen Stephen P. , Baruah Indrani K. , Ali Shahin S. , Shao Jonathan , Bukari Yahaya , Amoako-Attah Ishmael , Meinhardt Lyndel W. , Bailey Bryan A. 
Published: April 2023

Thread blight disease (TBD) is a serious emerging threat to global cacao production, causing leaf blight symptoms and necrosis in the canopy of infected trees. Though previously thought to be caused primarily by the fungus Marasmiellus scandens, recent work has revealed that a variety of pathogenic Marasmiaceae are responsible for TBD, with Marasmius tenuissimus identified as the dominant causal agent of TBD in Ghanaian cacao plantations. M. tenuissimus has also been reported in remote organic cacao plantations in the Amazonas Department of Peru, infecting 90 to 100% of the trees surveyed. Here, we have assembled and annotated the nuclear genome of M. tenuissimus isolate GH-37. This resource is a first attempt at a high-quality draft genome utilizing both second- and third-generation sequencing for M. tenuissimus and extends the current understanding of the evolution, ecology, and virulence of this pathogen. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Histological Comparative Analysis of Bowman Layer Grafts Procured Using 3 Different Techniques
Authors: Son Hyeck-Soo , Moon Loren , Wang Jiangxia , Eberhart Charles G. , Jun Albert S. , Srikumaran Divya , Soiberman Uri S. 
Published: April 2023

Purpose:
The objective of this study is to perform a histological analysis of Bowman layer (BL) grafts.

Methods:
BL grafts were procured from 13 human cadaver corneal tissues using 3 different donor preparation techniques. Subsequently, the grafts were fixed in 10% buffered formalin phosphate and embedded in paraffin. Hematoxylin and eosin sections of BL grafts were obtained and analyzed under a light microscope. BL and full graft thickness were measured using an image-processing software.

Results:
All 13 BL grafts contained residual anterior stromal tissue. BL stripping using Kelman-McPherson and Moorfield forceps (technique 3) achieved the thinnest graft thickness with a mean full graft thickness of 18.7 μm (95% confidence interval [CI], −9.8 to 47.2) at the thinnest point of the graft, whereas BL procurement using the Melles lamellar dissector (technique 2) led to the highest mean full graft thickness of 279.9 μm (95% CI, 251.4–308.5) even at the thinnest area of the graft. By contrast, BL dissection using a blunt dissector (technique 1) provided a mean full graft thickness of 70.2 μm (95% CI, 40.4–100.1) at the graft's thinnest point. Although peripheral graft tears occurred in 50%, 50%, and 100% of techniques 1, 2, and 3, respectively, intact 6.25-mm diameter BL grafts could be secured in 50%, 100%, and 80% of techniques 1, 2, and 3, respectively.

Conclusions:
None of the techniques used led to the procurement of pure BL grafts devoid of the anterior stroma. Peripheral scoring with a thin needle and tissue manipulation with Kelman-McPherson and Moorfield forceps led to the thinnest grafts in this study.
Survival analysis of malignant peripheral nerve sheath tumor: Experience of a tertiary center in Taiwan
Authors: Chang Yu-Wei , Liao Wen-Chieh 
Published: April 2023

Background:
This study aimed to analyze the demographic characteristics and prognostic factors of malignant peripheral nerve sheath tumor (MPNST) in a Taiwanese population. Single-center treatment outcomes were also presented.

Methods:
This retrospective cohort study analyzed the medical records of 54 patients with pathological diagnoses of MPNSTs from 2005 to 2021 at a single institution. The primary endpoint was the 5-year overall survival rate of MPNST, and the secondary endpoint was recurrence-free 5-year survival. Variables including patient characteristics, metastasis status at initial diagnosis, and surgical outcomes were analyzed with competing risk analysis.

Results:
Among all 41 eligible patients diagnosed with MPNST, female predominance was noted, and the median age at diagnosis was 44 years. The most common site of lesion was found at the trunk (46.34%), and eight patients were diagnosed with notable metastasis. Twelve patients were diagnosed with type 1 neurofibromatosis (NF1). The 5-year overall survival rate was 36.84% and the 5-year recurrence-free survival was 28.95%. Metastasis diagnosed at presentation, large lesion sizes, and recurrence were identified as significant poor prognostic factors of survival. Metastasis diagnosed at presentation was identified as the only significant risk factor of recurrence.

Conclusion:
In our series, metastasis diagnosed at presentation, large lesion sizes, and recurrence were identified as significant poor prognostic factors of survival. Metastasis was also identified as the only significant risk factor of recurrence. NF1-associated MPNSTs presented with significantly larger tumor sizes and additional treatment postoperatively did not significantly improve survival. The limitations of this study include its retrospective nature and sample size.
Understanding renal phosphate handling: unfinished business
Authors: Lederer Eleanor 
Published: April 2023

Purpose of review
The purpose of this review is to highlight the publications from the prior 12–18 months that have contributed significant advances in the field of renal phosphate handling.

Recent findings
The discoveries include new mechanisms for the trafficking and expression of the sodium phosphate cotransporters; direct link between phosphate uptake and intracellular metabolic pathways; interdependence between proximal tubule transporters; and the persistent renal expression of phosphate transporters in chronic kidney disease.

Summary
Discovery of new mechanisms for trafficking and regulation of expression of phosphate transporters suggest new targets for the therapy of disorders of phosphate homeostasis. Demonstration of stimulation of glycolysis by phosphate transported into a proximal tubule cell expands the scope of function for the type IIa sodium phosphate transporter from merely a mechanism to reclaim filtered phosphate to a regulator of cell metabolism. This observation opens the door to new therapies for preserving kidney function through alteration in transport. The evidence for persistence of active renal phosphate transport even with chronic kidney disease upends our assumptions of how expression of these transporters is regulated, suggests the possibility of alternative functions for the transporters, and raises the possibility of new therapies for phosphate retention.
Sagittal deformity of Garden type I and II geriatric femoral neck fractures is frequently misclassified by lateral radiographs
Authors: Tiee Madeline S. , Golz Andrew G. , Kim Andrew , Cohen Joseph B. , Summers Hobie D. , Alexander Anup J. , Lack William D. 
Published: April 2023

Objectives:
The objective of this study was to determine the validity and inter-rater reliability of radiographic assessment of sagittal deformity of femoral neck fractures.

Design:
This is a retrospective cohort study.

Setting:
Level 1 trauma center.

Patients/Participants:
Thirty-one patients 65 years or older who sustained low-energy, Garden type I/II femoral neck fractures imaged with biplanar radiographs and either computed tomography or magnetic resonance imaging were included.

Main Outcome Measurements:
Preoperative sagittal tilt was measured on lateral radiographs and compared with the tilt identified on advanced imaging. Fractures were defined as “high-risk” if posterior tilt was ≥20 degrees or anterior tilt was >10 degrees.

Results:
Of 31 Garden type I/II femoral neck fractures, advanced imaging identified 10 high-risk fractures including 8 (25.8%) with posterior tilt ≥20 degrees and 2 (6.5%) with anterior tilt >10 degrees. Overall, there was no significant difference between sagittal tilt measured using lateral radiographs and advanced imaging (P = 0.84), and the 3 raters had good agreement between their measurements of sagittal tilt on lateral radiographs (interclass correlation coefficient 0.79, 95% confidence interval [0.65, 0.88], P < 0.01). However, for high-risk fractures, radiographic measurements from lateral radiographs alone resulted in greater variability and underestimation of tilt by 5.2 degrees (95% confidence interval [−18.68, 8.28]) when compared with computed tomography/magnetic resonance imaging. Owing to this underestimation of sagittal tilt, the raters misclassified high-risk fractures as “low-risk” in most cases (averaging 6.3 of 10, 63%, range 6 − 7) when using lateral radiographs while low-risk fractures were rarely misclassified as high-risk (averaging 1.7 of 21, 7.9%, range 1 − 3, P = 0.01).

Conclusions:
Lateral radiographs frequently lead surgeons to misclassify high-risk sagittal tilt of low-energy femoral neck fractures as low-risk. Further research is necessary to improve the assessment of sagittal plane deformity for these injuries.

Level of Evidence:
Level IV diagnostic study.
Historical, Diagnostic, and Chromatic Treatment in Visual Snow Syndrome: A Retrospective Analysis
Authors: Han M. H. Esther , Ciuffreda Kenneth J. , Rutner Daniella 
Published: April 2023

SIGNIFICANCE
Visual snow syndrome is a relatively new medical condition, with presence of visual snow as the primary visual-perceptual symptom. Information from the present study will improve future clinical diagnostic and treatment aspects in this population.

PURPOSE
This study aimed to determine the historical, diagnostic, and treatment aspects in patients with documented visual snow syndrome/visual snow in an academic, optometric setting.

METHODS
A retrospective analysis was performed in patients (N = 40, aged 12 to 55 years) with documented visual snow syndrome/visual snow examined over a 4-year period. Information was collected by a detailed case history and the Visual Snow Syndrome Symptom Survey. Treatment assessment was performed using the Intuitive Colorimeter, and a wide selection of chromatic tints was assessed under the most provocative/exacerbating and other conditions.

RESULTS
Visual snow was typically constant and monochromatic, with it being present on average 6.43 years. Bright and dark surfaces were the most provocative/exacerbating/revealing conditions, along with the viewing of computer screens. The most common etiology was mild traumatic brain injury. The most common primary and secondary symptoms were photosensitivity and tinnitus, respectively. There was a high frequency of occurrence of oculomotor deficits, especially accommodative and vergence insufficiency (~40 to 50%). Eighty percent of the patients were prescribed a chromatic tint with subjective visual reduction of visual snow ranging from 15 to 100% (mean, 45%).

CONCLUSIONS
The present information will help in understanding this unusual medicoperceptual condition, especially with respect to simple treatment frequently using readily available chromatic tints.
Medical Cannabis in the Treatment of Parkinson’s Disease
Authors: Aladeen Traci S. , Mattle Anna G. , Zelen Kory , Mesha Moustafa , Rainka Michelle M. , Geist Tanya , Myers Bennett , Mechtler Laszlo 
Published: April 2023

Objectives
Medical cannabis (MC) has recently garnered interest as a potential treatment for neurologic diseases, including Parkinson’s disease (PD). A retrospective chart review was conducted to explore the impact of MC on the symptomatic treatment of patients with PD.

Methods
Patients with PD treated with MC in the normal course of clinical practice were included (n = 69). Data collected from patient charts included MC ratio/formulation changes, PD symptom changes after initiation of MC, and adverse events (AEs) from MC use. Information regarding changes in concomitant medications after MC initiation, including opioids, benzodiazepines, muscle relaxants, and PD medications, was also collected.

Results
Most patients were initially certified for a 1:1 (∆9-tetrahydrocannabinol:cannabidiol) tincture. Eight-seven percent of patients (n = 60) were noted to exhibit an improvement in any PD symptom after starting MC. Symptoms with the highest incidence of improvement included cramping/dystonia, pain, spasticity, lack of appetite, dyskinesia, and tremor. After starting MC, 56% of opioid users (n = 14) were able to decrease or discontinue opioid use with an average daily morphine milligram equivalent change from 31 at baseline to 22 at the last follow-up visit. The MC was well-tolerated with no severe AEs reported and low rate of MC discontinuation due to AEs (n = 4).

Conclusions
The MC may improve motor and nonmotor symptoms in patients with PD and may allow for reduction of concomitant opioid medication use. Large, placebo-controlled, randomized studies of MC use in patients with PD are required.
GASTRIC ACIDITY IN DIFFERENT PHENOTYPES OF SYMPTOMATIC GASTROESOPHAGEAL REFLUX DISEASE
Authors: Gardner Jerry D. 
Published: April 2023

ABSTRACTBackgroundThe present report examines gastric pH from normal subjects and different gastroesophageal reflux disease (GERD) phenotypes to compare the distributions of gastric pH values and of changes in gastric acid concentrations to those reported previously for esophageal pH from the same groups of subjects. I also examined total esophageal acidity as a function of total gastric acidity in the different groups of subjects.MethodsI analyzed 24-hour gastric and esophageal pH recordings from normal subjects and subjects with a particular GERD phenotype to calculate total integrated acidity and total time pH<4. I also examined gastric pH recordings for the distributions of gastric pH values and the distributions of changes in gastric acidity.ResultsThere were different distributions for gastric pH, but virtually identical distributions of changes in gastric acid concentrations in the different groups of subjects. There was a significant positive relationship between total integrated esophageal acidity and total integrated gastric acidity in different GERD phenotypes, but not in normal subjects. The slope of the line relating integrated esophageal acidity to gastric acidity correlated directly with the responses of different GERD phenotypes to PPI treatment reported previously by others.ConclusionsIt seems possible esophageal acidity and gastric acidity can influence each other and by so doing account for the variation in the differences in the distributions of values of pH and acid concentrations among normal subjects and different GERD phenotypes. Furthermore, the strength of the relationship between esophageal and gastric acidity can determine the response to PPI treatment.