bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous photoautotrophic production of DHA and EPA by Tisochrysis lutea and Microchloropsis salina in co-culture
Authors: Thurn Anna-Lena , Stock Anna , Gerwald Sebastian , Weuster-Botz Dirk 
Published: December 2022

AbstractMarine microalgae have received much attention as a sustainable source of the two health beneficial omega-3-fatty acids docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5). However, photoautotrophic monocultures of microalgae can only produce either DHA or EPA enriched biomass. An alternative may be the photoautotrophic co-cultivation of Tisochrysis lutea as DHA-producer with Microchloropsis salina for simultaneous EPA production to obtain EPA- and DHA-rich microalgae biomass in a nutritionally balanced ratio. Photoautotrophic co-cultivation processes of T. lutea and M. salina were studied, applying scalable and fully controlled lab-scale gas-lift flat-plate photobioreactors with LED illumination for dynamic climate simulation of a repeated sunny summer day in Australia [day–night cycles of incident light (PAR) and temperature]. Monocultures of both marine microalgae were used as reference batch processes. Differences in the autofluorescence of both microalgae enabled the individual measurement, of cell distributions in co-culture, by flow cytometry. The co-cultivation of T. lutea and M. salina in artificial sea water with an inoculation ratio of 1:3 resulted in a balanced biomass production of both microalgae simultaneously with a DHA:EPA ratio of almost 1:1 (26 mgDHA gCDW−1, and 23 mgEPA gCDW−1, respectively) at harvest after depletion of the initially added fertilizer. Surprisingly, more microalgae biomass was produced within 8 days in co-cultivation with an increase in the cell dry weight (CDW) concentration by 31%, compared to the monocultures with the same amount of light and fertilizer. What is more, DHA-content of the microalgae biomass was enhanced by 33% in the co-culture, whereas EPA-content remained unchanged compared to the monocultures.
Graphical Abstract
Evaluation of integrin αvβ3-targeted imaging for predicting disease progression in patients with high-risk differentiated thyroid cancer (using 99mTc-3PRGD2)
Authors: Liang Yiqian , Jia Xi , Wang Yuanbo , Liu Yan , Yao Xiaobao , Bai Yanxia , Han Peng , Chen Si , Yang Aimin , Gao Rui 
Published: December 2022

Abstract
Background
High-risk differentiated thyroid cancer (DTC) needs effective early prediction tools to improving clinical management and prognosis. This cohort study aimed to investigate the prognostic impact of 99mTc-PEG4-E[PEG4-c(RGDfK)]2 (99mTc-3PRGD2) SPECT/CT in high-risk DTC patients after initial radioactive iodine (RAI) therapy.

Methods
Thirty-three patients with high-risk DTC were prospectively recruited; all patients underwent total thyroidectomy and received 99mTc-3PRGD2 SPECT/CT before RAI ablation. Follow-up was done with serological and imaging studies. The correlation between 99mTc-3PRGD2 avidity and remission rate for initial RAI therapy was evaluated using logistic regression analysis. The prognostic value of 99mTc-3PRGD2 SPECT/CT was evaluated by Kaplan-Meier curve and Cox regression analysis.

Results
99mTc-3PRGD2 avidity was significantly correlated with the efficacy of initial RAI ablation and an effective predictor for non-remission in high-risk DTC (OR = 9.36; 95% CI = 1.10–79.83; P = 0.041). 99mTc-3PRGD2 avidity was associated with poor prognosis in patients with high-risk DTC and an independent prognostic factor for shorter progression-free survival (PFS) (HR = 9.47; 95% CI = 1.08–83.20; P = 0.043). Survival analysis, which was performed between DTC patients with concordant (131I positive/99mTc-3PRGD2 positive) and discordant (131I negative/99mTc-3PRGD2 positive) lesions, indicated that patients with concordant lesions had significantly better PFS than those with discordant lesions (P = 0.022). Moreover, compared with repeated RAI, additional surgery or targeted therapy with multikinase inhibitors could lead to a higher rate of remission in 99mTc-3PRGD2-positive patients with progressive disease.

Conclusions
99mTc-3PRGD2 SPECT/CT is a useful modality in predicting progression of the disease after initial RAI and guiding further treatment in high-risk DTC patients.
Treating Acute EXacerbations of COPD with Chinese HerbAL MedIcine to aid AntiBiotic Use Reduction (EXCALIBUR): study protocol of a randomised double-blind, placebo-controlled feasibility trial
Authors: Hu Xiao-Yang , Oliver Tom , Willcox Merlin , Simpson Catherine , Thorne Kerensa , Trill Jeanne , Francis Nick , Stuart Beth , Thomas Michael , Little Paul , Liu Jian-Ping , Griffiths Gareth , Moore Michael 
Published: December 2022

AbstractBackgroundAcute exacerbations of chronic obstructive pulmonary disease (AECOPD) are a major reason for consultations in primary care, hospital admissions, deterioration in function, and mortality. Despite the majority of exacerbations not being caused by bacteria, as many as 70% of patients who present in UK primary care with AECOPD are prescribed antibiotics as part of standard care. However, finding effective non-antibiotic treatments for COPD exacerbations is a priority to reduce antibiotic use. The Chinese herbal medicine Shufeng Jiedu® (SFJD) has the potential to reduce treatment failure and duration of hospital stay. This study aims to determine the feasibility of conducting a fully powered randomised, double blind, placebo-controlled clinical trial on SFJD for AECOPD in UK primary care.MethodsThis study is a phase III, two-arm individually double blind, randomised, placebo-controlled feasibility trial with nested qualitative study, coordinated by the Southampton Clinical Trial Unit (SCTU). Patients aged ≥ 40 years, with a current AECOPD, presenting with increased sputum purulence/volume, or breathlessness, and for whom the GP is considering use of antibiotics, will be eligible to participate. We aim to recruit seven eligible participants per month and randomise them to receive either the patent Chinese herbal medicine SFJD capsules or placebo for 14 consecutive days and to follow-up for 12 weeks. The primary outcomes include the feasibility of recruitment, study retention, and the percentage of diary completion.DiscussionIf this trial demonstrates the feasibility of recruitment, delivery, and follow-up, we will seek funding for a fully powered placebo-controlled trial of SFJD for the treatment of AECOPD in primary care.Trial registrationThis trial is registered via ISRCTN on 1 July 2021, identifier: ISRCTN26614726.
The association between dietary acid load and body composition in physical education students aged 18–25 years
Authors: Mansordehghan Maryam , Daneshzad Elnaz , Basirat Vahid , Gargari Bahram Pourghassem , Rouzitalab Tohid 
Published: December 2022

AbstractAimTo find the association between dietary acid load (DAL) and body composition in physical education students.MethodsThis study was carried out on 207 students of both genders aged 18–25 years. DAL was calculated based on potential renal acid load (PRAL) and net endogenous acid production (NEAP) methods. Anthropometric indices were measured. Bioelectric impedance was used to assess body composition and other related items.ResultsThe mean score of NEAP and PRAL was 80.18 ± 31.30 and 33.94 ± 22.11, respectively. The mean weight and fat mass of subjects were 64.05 ± 9.72 kg and 20.28 ± 0.67 kg, respectively. Participants in the highest tertile of PRAL had a higher weight (64.56 ± 1.14 kg) in comparison with participants in the lowest tertile (61.65 ± 1.19 kg) (P = 0.027). After adjusting for confounders, a significant positive association was found between NEAP score and hip circumference (β = 0.206,P = 0.039), body mass index (β = 0.214,P = 0.031), fat mass (β = 0.218,P = 0.001) and body adiposity index (β = 0.182,P = 0.037). Furthermore, a statistically significant negative association was observed between total body water and NEAP score (β = − 0.217,P = 0.001) and the percentage of fat-free mass and NEAP (β = − 0.229,P = 0.001).ConclusionIndividuals with a higher DAL score may have a higher weight, fat mass and hip circumference and a lower fat-free mass. In addition, there might be an association between DAL and obesity.
Abscess formation caused by a foreign body reaction to a poly-d, l-lactic acid (PDLLA) absorbable plate for zygomaticomaxillary complex (ZMC) fracture
Authors: Tamura Fumikazu , Sasaki Kaoru , Oshima Junya , Hanihara Hironao , Myojo Risa , Shibuya Yoichiro , Sasaki Masahiro , Aihara Yukiko , Sekido Mitsuru 
Published: December 2022

Abstract
Background
The poly-d, l-lactic acid absorbable plate does not contain poly l-lactic acid, which is the main factor in foreign body reactions; therefore, with use of this plate, a foreign body reaction is unlikely. In fact, few reports have been published on removal of a poly-d, l-lactic acid absorbable plate owing to a foreign body reaction.

Case presentation
We report a case of abscess formation that led finally to the removal of a poly-d, l-lactic acid absorbable plate. A 45-years-old man with a left zygomaticomaxillary complex fracture sustained during a fall underwent open reduction using the poly-d, l-lactic acid absorbable plate. Fourteen months after the operation, he was admitted to our hospital with swelling and redness of the left lateral part of the upper eyelid. Upon incision of the swelling, we found a large amount of purulent drainage and the plate in broken fragments and removed as many of the fragments as possible. The swelling was improved after drainage of the incision twice and use of antibiotics, and the bacterial culture results were negative. No recurrence or aggravation was observed after the drainage.

Conclusions
We suspected sterile abscess formation secondary to a foreign body reaction to the poly-d, l-lactic acid absorbable plate. Given that the poly-d, l-lactic acid absorbable plate is absorbed over a period of more than 1 year postoperatively, long-term follow-up of injuries treated using the plate may be required. Furthermore, surgical intervention, such as drainage or removal, should be considered if the foreign body reaction symptoms are severe.
A review of the mathematical modeling of equilibrium and nonequilibrium hypersonic flows
Authors: Zhang Wenqing , Zhang Zhijun , Wang Xiaowei , Su Tianyi 
Published: December 2022

AbstractThis paper systematically reviews the mathematical modeling based on the computational fluid dynamics (CFD) method of equilibrium and nonequilibrium hypersonic flows. First, some physicochemical phenomena in hypersonic flows (e.g., vibrational energy excitation and chemical reactions) and the flow characteristics at various altitudes (e.g., thermochemical equilibrium, chemical nonequilibrium, and thermochemical nonequilibrium) are reviewed. Second, the judgment rules of whether the CFD method can be applied to hypersonic flows are summarized for accurate numerical calculations. This study focuses on the related numerical models and calculation processes of the CFD method in a thermochemical equilibrium flow and two nonequilibrium flows. For the thermochemical equilibrium flow, the governing equations, chemical composition calculation methods, and related research on the thermodynamic and transport properties of air are reviewed. For the nonequilibrium flows, the governing equations that include one-, two-, and three-temperature models are reviewed. The one-temperature model is applied to a chemical nonequilibrium flow, whereas the two- and three-temperature models are applied to a thermochemical nonequilibrium flow. The associated calculations and numerical models of the thermodynamic and transport properties, chemical reaction sources, and energy transfers between different energy modes of the three models are presented in detail. Finally, the corresponding numerical models of two special wall boundary conditions commonly used in hypersonic flows (i.e., slip boundary conditions and catalytic walls) and related research, are reviewed.
Pediatric versus adult maxillofacial fractures
Authors: El-Anwar Mohammad Waheed , Elshiekh Ezzeddin , Awad Ali , Elsayed Abdalla Mohammed , Alshawadfy Mohamed 
Published: December 2022

Abstract
Objective
To study, compare, and analyze the differences and the detailed features of the maxillofacial fractures in adults versus children.

Patients and methods
This prospective comparative study was conducted on patients who had traumatic maxillofacial fractures. Patients were categorized into 2 groups: the pediatric group and the adult group. History taking including the type of trauma, examination, computed tomography (CT) maxillofacial, fracture management, and outcome evaluation was done for all patients.

Results
Within included 82 patients (42 adults, 40 children), males were the most affected in adult and pediatric groups and the most affected age was between 19 and 27 years (29.2%). Motor vehicle accident (MVA) was the most common cause in both the adult group (66.66%) and the pediatric group (60%). But fall represents a significant cause in children (17.5%) with a significant difference between the two groups (p = 0.038). The mandibular fracture was the most common fracture in pediatric (65%) and adult (38.1%) groups with a significant difference between both groups regarding different sites of trauma (p = 0.017). Surgical intervention was performed in 80% of pediatric patients and 92.23% with a significant difference (p = 0.035).

Conclusion
MVA is the main etiology of maxillofacial fractures in children and adults, but fall is an important cause of pediatric trauma. Pediatric maxillofacial fractures below school age are a rare event with increasing incidence with age. Mandibular fractures are the most common site in the adult and pediatric populations. Most cases of maxillofacial fractures are surgically treated with OR/IF with good results but conservative management is used more in pediatric than adult fractures.
Crizotinib in Combination With Chemotherapy for Pediatric Patients With ALK+ Anaplastic Large-Cell Lymphoma: The Results of Children's Oncology Group Trial ANHL12P1
Authors: Lowe Eric J. , Reilly Anne F. , Lim Megan S. , Gross Thomas G. , Saguilig Lauren , Barkauskas Donald A. , Wu Rui , Alexander Sarah , Bollard Catherine M. 
Published: December 2022

PURPOSE Arm crizotinib (CZ) of the Children's Oncology Group trial ANHL12P1 (ClinicalTrials.gov identifier: NCT01979536 ) examined the efficacy and toxicity of adding CZ to standard chemotherapy for children with newly diagnosed, nonlocalized ALK+ CD30+ anaplastic large-cell lymphoma (ALCL). PATIENTS AND METHODS Between 2013 and 2019, 66 enrolled children received CZ with chemotherapy. Patients received a 5-day prophase followed by six chemotherapy cycles at 21-day intervals with CZ administered twice daily during each 21-day cycle. The study was temporarily closed for two periods (total 12 months) to evaluate toxicity, during which CZ was discontinued. Measurements of NPM-ALK fusion transcripts in peripheral blood were performed at diagnosis for minimal disseminated disease (MDD). RESULTS The 2-year event-free survival (EFS) is 76.8% (95% CI, 68.5 to 88.1) and the 2-year overall survival is 95.2% (95% CI, 85.7 to 98.4). Fifteen patients relapsed and one patient died; median time to relapse was 7.4 months from diagnosis, with relapses occurring after chemotherapy was complete. The 66 patients completed 384 cycles of chemotherapy. Thirteen of the 66 patients experienced a grade 2+ thromboembolic adverse event (19.7%; 95% CI, 11.1 to 31.3). In the 25 patients who received mandated prophylactic anticoagulation, there were two thromboembolic events (8.0%; 95% CI, 0.01 to 26). Patients with negative MDD had a superior outcome, with an EFS of 85.6% (95% CI, 68.6 to 93.8); positive MDD was associated with a lower EFS of 58.1% (95% CI, 33.4 to 76.4). CONCLUSION Arm CZ of ANHL12P1 demonstrated that the addition of CZ to standard treatment prevented relapses during therapy for children with ALCL, MDD predicted EFS, and the addition of CZ resulted in unexpected thromboembolic events. Overall survival and EFS rates are consistent with the highest reported outcomes for children with ALCL.
Translational control by maternal Nanog promotes oogenesis and early embryonic development
Authors: He Mudan , Jiao Shengbo , Zhang Ru , Ye Ding , Wang Houpeng , Sun Yonghua 
Published: December 2022

ABSTRACT
Many maternal mRNAs are translationally repressed during oocyte development and spatio-temporally activated during early embryogenesis, which is crucial for oocyte and early embryo development. By analyzing maternal mutants of nanog (Mnanog) in zebrafish, we demonstrated that Nanog tightly controls translation of maternal mRNA during oogenesis via transcriptional repression of eukaryotic translation elongation factor 1 alpha 1, like 2 (eef1a1l2). Loss of maternal Nanog led to defects of egg maturation, increased endoplasmic reticulum stress, and an activated unfold protein response, which was caused by elevated translational activity. We further demonstrated that Nanog, as a transcriptional repressor, represses the transcription of eefl1a1l2 by directly binding to the eef1a1l2 promoter in oocytes. More importantly, depletion of eef1a1l2 in nanog mutant females effectively rescued the elevated translational activity in oocytes, oogenesis defects and embryonic defects of Mnanog embryos. Thus, our study demonstrates that maternal Nanog regulates oogenesis and early embryogenesis through translational control of maternal mRNA via a mechanism whereby Nanog acts as a transcriptional repressor to suppress transcription of eef1a1l2.
Resistance Exercise Training Increases Muscle Mass and Strength in Prostate Cancer Patients on Androgen Deprivation Therapy
Authors: HOUBEN LISANNE H. P. , OVERKAMP MAARTEN , VAN KRAAIJ PUCK , TROMMELEN JORN , VAN ROERMUND JOEP G. H. , DE VRIES PETER , DE LAET KEVIN , VAN DER MEER SASKIA , MIKKELSEN ULLA R. , VERDIJK LEX B. , VAN LOON LUC J. C. , BEIJER SANDRA , BEELEN MILOU 
Published: December 2022

ABSTRACT

Purpose
This study aimed to assess the effects of 20 wk resistance exercise training with or without protein supplementation on body composition, muscle mass, muscle strength, physical performance, and aerobic capacity in prostate cancer patients receiving androgen deprivation therapy (ADT).

Methods
Sixty prostate cancer patients receiving ADT were randomly assigned to perform 20 wk of resistance exercise training with supplementation of 31 g whey protein (EX + PRO, n = 30) or placebo (EX + PLA, n = 30), consumed immediately after exercise and every night before sleep. A separate control group (CON, n = 36) only received usual care. At baseline and after 20 wk, body composition (dual-energy x-ray absorptiometry), muscle mass (computed tomography scan), muscle strength (1-repetition maximum strength tests), physical performance (Timed Up and Go Test, 30-Second Chair Stand Test, and Stair Climb Test), aerobic capacity (cardiopulmonary exercise test), and habitual dietary intake (food diary) were assessed. Data were analyzed using a two-factor repeated-measures ANOVA.

Results
Over time, muscle mass and strength increased in EX + PRO and EX + PLA and decreased in CON. Total fat mass and fat percentage increased in EX + PRO and CON, but not in EX + PLA. Physical performance did not significantly change over time in either group. Aerobic capacity was maintained in EX + PLA, but it decreased in EX + PRO and CON. Habitual protein intake (without supplements) averaged >1.0 g·kg body weight−1·d−1, with no differences over time or between groups.

Conclusions
In prostate cancer patients, resistance exercise training counteracts the adverse effects of ADT on body composition, muscle mass, muscle strength, and aerobic capacity, with no additional benefits of protein supplementation.
Effects of spatial and temporal resolution on cardiovascular magnetic resonance feature tracking measurements using a simple realistic numerical phantom
Authors: Adams David M , Boubertakh Redha , Miquel Marc Eric 
Published: December 2022

Objectives To develop a single-slice numerical phantom with known myocardial motion, at several temporal and in-plane spatial resolutions, for testing and comparison of Cardiovascular Magnetic Resonance (CMR) feature tracking (FT) software. Methods The phantom was developed based on CMR acquisitions of one volunteer (acquired cine, tagging cine, T1 map, T2 map, proton density weighted image). The numerical MRI simulator JEMRIS was used, and the phantom was generated at several in-plane spatial resolutions (1.4 × 1.4 mm2 to 3.0 × 3.0 mm2) and temporal resolutions (20 to 40 cardiac phases). Two feature tracking software packages were tested: Medical Image Tracking Toolbox (MITT) and two versions of cvi42 (v5.3.8 and v5.13.7). The effect of resolution on strain results was investigated with reference to ground-truth radial and circumferential strain. Results Peak radial strain was consistently undermeasured more for cvi42 v5.13.7 than for v5.3.8. Increased pixel size produced a trend of increased difference from ground-truth peak strain, with the largest changes for cvi42 obtained using v5.13.7 between 1.4 × 1.4 mm2 and 3.0 × 3.0 mm2, at 9.17 percentage points (radial) and 8.42 percentage points (circumferential). Conclusions The results corroborate the presence of intervendor differences in feature tracking results and show the magnitude of strain differences between software versions. Advances in knowledge This study shows how temporal and in-plane spatial resolution can affect feature tracking with reference to the ground-truth strain of a numerical phantom. Results reaffirm the need for numerical phantom development for the validation and testing of FT software.
The Importance of Separating the Probability of Committing and Detecting Misstatements in the Restatement Setting
Authors: Barton F. Jane , Burnett Brian M. , Gunny Katherine , Miller Brian P. 
Published: December 2022

This study demonstrates the importance of separating the probabilities of misstatement occurrence and detection when examining financial statement restatements. Despite the many benefits of examining the probability of restatements using traditional logistic models, interpretations of these models are clouded by partial observability—only subsequently detected misstatements are observable. We propose addressing this often overlooked issue by implementing a bivariate probit model with partial observability. We demonstrate the importance of separating these latent probabilities by re-examining three prior restatement studies and show the importance of separating the occurrence and detection probabilities. Our evidence suggests that future studies interested in restatements as a measure of accounting quality should consider implementing bivariate probit models as one way to address the partial observability inherent in this setting. This paper was accepted by Brian Bushee, accounting. Funding: B. P. Miller gratefully acknowledges financial support from the Sam Frumer Professorship. Supplemental Material: Data and the internet appendix are available at https://doi.org/10.1287/mnsc.2022.4627 .