bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased BMP-Smad signaling does not affect net bone mass in long bones
Authors: Omi Maiko , Koneru Tejaswi , Lyu Yishan , Haraguchi Ai , Kamiya Nobuhiro , Mishina Yuji 
Published: March 2023

Bone morphogenetic proteins (BMPs) have been used for orthopedic and dental application due to their osteoinductive properties; however, substantial numbers of adverse reactions such as heterotopic bone formation, increased bone resorption and greater cancer risk have been reported. Since bone morphogenetic proteins signaling exerts pleiotropic effects on various tissues, it is crucial to understand tissue-specific and context-dependent functions of bone morphogenetic proteins. We previously reported that loss-of-function of bone morphogenetic proteins receptor type IA (BMPR1A) in osteoblasts leads to more bone mass in mice partly due to inhibition of bone resorption, indicating that bone morphogenetic protein signaling in osteoblasts promotes osteoclast function. On the other hand, hemizygous constitutively active (ca) mutations for BMPR1A (caBmpr1awt/+) in osteoblasts result in higher bone morphogenetic protein signaling activity and no overt skeletal changes in adult mice. Here, we further bred mice for heterozygous null for Bmpr1a (Bmpr1a+/−) and homozygous mutations of caBmpr1a (caBmpr1a+/+) crossed with Osterix-Cre transgenic mice to understand how differences in the levels of bone morphogenetic protein signaling activity specifically in osteoblasts contribute to bone phenotype. We found that Bmpr1a+/−, caBmpr1awt/+ and caBmpr1a+/+ mice at 3 months of age showed no overt bone phenotypes in tibiae compared to controls by micro-CT and histological analysis although BMP-Smad signaling is increased in both caBmpr1awt/+ and caBmpr1a+/+ tibiae and decreased in the Bmpr1a+/− mice compared to controls. Gene expression analysis demonstrated that slightly higher levels of bone formation markers and resorption markers along with levels of bone morphogenetic protein-Smad signaling, however, there was no significant changes in TRAP positive cells in tibiae. These findings suggest that changes in bone morphogenetic protein signaling activity within differentiating osteoblasts does not affect net bone mass in the adult stage, providing insights into the concerns in the clinical setting such as high-dose and unexpected side effects of bone morphogenetic protein application.
Morphological change and differential proteomics analysis of gill in Mytilus coruscus under starvation
Authors: Liang Ze-Wei , Li Si-Yuan , Zhang Xiao-Lin , Chen Chuan-Yue , Sun Wen-Jing , Gu Zhong-Qi , Huang Ji , He Jian-Yu , Qi Peng-Zhi , Guo Bao-Ying , Liao Zhi , Yan Xiao-Jun 
Published: March 2023

Mytilus coruscus is a dominant shellfish in the Yangtze estuary and its adjacent sea area. Food deprivation often occurs during their growth due to fluctuations in algal abundance caused by seasonal freshwater flushing and high-density aquaculture mode. To investigate the coping strategies of M. coruscus to starvation stress, electron microscopy and differential proteomic analysis were performed on the critical feeding organ gill of the mussels after 9 days of starvation. The electron microscopy results showed that the cilia of the mussel gills were dissolved, and the gaps between gill filaments widened under starvation. Differential proteomic analysis revealed that phagocytosis-related proteins such as ATPeV1E, ATPeV1C, LAMP1_2 and CTSL were significantly upregulated, and the phagocytosis pathway was significantly enriched (p &lt; 0.05). In addition, the corin content in gill and myeloperoxidase level as well as the number of dead cells in blood were both significantly increased (p &lt; 0.05). What’s more, proteomic data suggested that immune maintenance, cellular transport and metabolism related pathways were significantly enriched, which illustrated an immune and metabolism responses under starvation. This study reveals for the first time that phagocytosis functions as an essential strategy for M. coruscus to cope with starvation, which provides new scientific knowledge and a theoretical basis for understanding the adaptation mechanisms of mussel to starvation and for rational optimization of mussel culture patterns.
Differences in phyllosphere microbiomes among different Populus spp. in the same habitat
Authors: Liu Jiaying , Zhang Weixi , Liu Yuting , Zhu Wenxu , Yuan Zhengsai , Su Xiaohua , Ding Changjun 
Published: March 2023

IntroductionThe above-ground parts of terrestrial plants are collectively known as the phyllosphere. The surface of the leaf blade is a unique and extensive habitat for microbial communities. Phyllosphere bacteria are the second most closely associated microbial group with plants after fungi and viruses, and are the most abundant, occupying a dominant position in the phyllosphere microbial community. Host species are a major factor influencing the community diversity and structure of phyllosphere microorganisms.MethodsIn this study, six Populus spp. were selected for study under the same site conditions and their phyllosphere bacterial community DNA fragments were paired-end sequenced using 16S ribosomal RNA (rRNA) gene amplicon sequencing. Based on the distribution of the amplicon sequence variants (ASVs), we assessed the alpha-diversity level of each sample and further measured the differences in species abundance composition among the samples, and predicted the metabolic function of the community based on the gene sequencing results.ResultsThe results revealed that different Populus spp. under the same stand conditions resulted in different phyllosphere bacterial communities. The bacterial community structure was mainly affected by the carbon and soluble sugar content of the leaves, and the leaf nitrogen, phosphorus and carbon/nitrogen were the main factors affecting the relative abundance of phyllosphere bacteria.DiscussionPrevious studies have shown that a large proportion of the variation in the composition of phyllosphere microbial communities was explained by the hosts themselves. In contrast, leaf-borne nutrients were an available resource for bacteria living on the leaf surface, thus influencing the community structure of phyllosphere bacteria. These were similar to the conclusions obtained in this study. This study provides theoretical support for the study of the composition and structure of phyllosphere bacterial communities in woody plants and the factors influencing them.
Association of early childhood constipation with the risk of autism spectrum disorder in Taiwan: Real-world evidence from a nationwide population-based cohort study
Authors: Lee Yi-Feng , Wu Meng-Che , Ma Kevin Sheng-Kai , Huang Jing-Yang , Wei James Cheng-Chung 
Published: March 2023

BackgroundAutism spectrum disorder (ASD) is a neurodevelopmental problem that presents with limited interests, repetitive behaviors, and deficits in reciprocal communication and social interactions. Mounting evidence indicates that an imbalanced gut microbiota contributes to autism via the gut-brain axis. Constipation may result in alteration of the gut microbiota. The clinical influence of constipation on ASD has not been fully researched. Thus, in this study we aimed to evaluate whether early childhood constipation influenced the risk of developing ASD using a nationwide population-based cohort study.MethodsWe identified 12,935 constipated children aged 3 years or younger from the National Health Insurance Research Database (NHIRD) in Taiwan from 1997 to 2013. Non-constipated children were also selected from the database and propensity score matching of age, gender, and underlying comorbidities was conducted with a ratio of 1:1. Kaplan–Meier analysis was applied to determine different levels of constipation severity and cumulative incidence of autism. Subgroup analysis was also applied in this study.ResultsThe incidence rate of ASD was 12.36 per 100,000 person-months in the constipation group, which was higher than the rate of 7.84 per 100,000 person-months noted in the non-constipation controls. Constipated children had a significantly higher risk of autism when compared to the non-constipation group (crude relative risk = 1.458, 95% CI = 1.116–1.904; adjusted hazard ratio = 1.445, 95% CI = 1.095–1.907).Moreover, among constipated children, a higher number of laxative prescriptions, male gender, constipation during infancy, and atopic dermatitis were significantly associated with higher risks of ASD when compared to the non-constipation group.ConclusionConstipation in early childhood was correlated with a significantly increased risk of ASD. Clinicians should pay attention to the possibility of ASD in constipated children. Further research is necessary to study the possible pathophysiological mechanisms of this association.
Applying the core-satellite species concept: Characteristics of rare and common riverine dissolved organic matter
Authors: Stadler Masumi , Barnard Malcolm A. , Bice Kadir , de Melo Michaela L. , Dwivedi Dipankar , Freeman Erika C. , Garayburu-Caruso Vanessa A. , Linkhorst Annika , Mateus-Barros Erick , Shi Cheng , Tanentzap Andrew J. , Meile Christof 
Published: March 2023

IntroductionDissolved organic matter (DOM) composition varies over space and time, with a multitude of factors driving the presence or absence of each compound found in the complex DOM mixture. Compounds ubiquitously present across a wide range of river systems (hereafter termed core compounds) may differ in chemical composition and reactivity from compounds present in only a few settings (hereafter termed satellite compounds). Here, we investigated the spatial patterns in DOM molecular formulae presence (occupancy) in surface water and sediments across 97 river corridors at a continental scale using the “Worldwide Hydrobiogeochemical Observation Network for Dynamic River Systems—WHONDRS” research consortium.MethodsWe used a novel data-driven approach to identify core and satellite compounds and compared their molecular properties identified with Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS).ResultsWe found that core compounds clustered around intermediate hydrogen/carbon and oxygen/carbon ratios across both sediment and surface water samples, whereas the satellite compounds varied widely in their elemental composition. Within surface water samples, core compounds were dominated by lignin-like formulae, whereas protein-like formulae dominated the core pool in sediment samples. In contrast, satellite molecular formulae were more evenly distributed between compound classes in both sediment and water molecules. Core compounds found in both sediment and water exhibited lower molecular mass, lower oxidation state, and a higher degree of aromaticity, and were inferred to be more persistent than global satellite compounds. Higher putative biochemical transformations were found in core than satellite compounds, suggesting that the core pool was more processed.DiscussionThe observed differences in chemical properties of core and satellite compounds point to potential differences in their sources and contribution to DOM processing in river corridors. Overall, our work points to the potential of data-driven approaches separating rare and common compounds to reduce some of the complexity inherent in studying riverine DOM.
Complications of extraperitoneal robot-assisted radical prostatectomy in high-risk prostate cancer: A single high-volume center experience
Authors: Paladini Alessio , Cochetti Giovanni , Felici Graziano , Russo Miriam , Saqer Eleonora , Cari Luigi , Bordini Stefano , Mearini Ettore 
Published: March 2023

IntroductionThe role of robot-assisted radical prostatectomy (RARP) in high-risk prostate cancer (PCa) has been debated over the years, but it appears safe and effective in selected patients. While the outcomes of transperitoneal RARP for high-risk PCa have been already widely investigated, data on the extraperitoneal approach are scarcely available. The primary aim of this study is to evaluate intra- and postoperative complications in a series of patients with high-risk PCa treated by extraperitoneal RARP (eRARP) and pelvic lymph node dissection. The secondary aim is to report oncological and functional outcomes.MethodsData of patients who underwent eRARP for high-risk PCa were prospectively collected from January 2013 to September 2021. Intraoperative and postoperative complications were recorded, as also perioperative, functional, and oncological outcomes. Intraoperative and postoperative complications were classified by employing Intraoperative Adverse Incident Classification by the European Association of Urology and the Clavien–Dindo classification, respectively. Univariate and multivariate analyses were performed to evaluate a potential association between clinical and pathological features and the risk of complications.ResultsA total of 108 patients were included. The mean operative time and estimated blood loss were 183.5 ± 44 min and 115.2 ± 72.4 mL, respectively. Only two intraoperative complications were recorded, both grade 3. Early complications were recorded in 15 patients, of which 14 were of minor grade, and 1 was grade IIIa. Late complications were diagnosed in four patients, all of grade III. Body mass index (BMI) &gt; 30 kg/m2, Prostate-Specific Antigen (PSA) &gt; 20 ng/mL, PSA density &gt;0.15 ng/mL2, and pN1 significantly correlated with a higher rate of overall postoperative complications. Moreover, BMI &gt;30 kg/m2, PSA &gt;20 ng/mL, and pN1 significantly correlated with a higher rate of early complications, while PSA &gt;20 ng/mL, prostate volume &lt;30 mL, and pT3 were significantly associated with a higher risk of late complications. In multivariate regression analysis, PSA &gt;20 ng/mL significantly correlated with overall postoperative complications, while PSA &gt; 20 and pN1 correlated with early complications. Urinary continence and sexual potency were restored in 49.1%, 66.7%, and 79.6% of patients and in 19.1%, 29.9%, and 36.2% of patients at 3, 6, and 12 months, respectively.ConclusionseRARP with pelvic lymph node dissection in patients with high-risk PCa is a feasible and safe technique, resulting in only a few intra- and postoperative complications, mostly of low grade.
Computed tomographic quantitative evaluation of common bile duct size in normal dogs: A reference range study considering body weight
Authors: Kim Yein , Kim Sung-Soo , Kwon Danbee , Im Deokho , Lee Kichang , Yoon Hakyoung 
Published: March 2023

IntroductionCommon bile duct (CBD) measurements are important for the evaluation of biliary systemic disorders. However, in veterinary medicine, reference ranges for specific body weights (BW) and correlation between CBD diameter and BW have not been studied. This study aimed to establish normal reference ranges of CBD diameter for different BW groups and to analyse correlation between CBD diameter and BW in dogs without hepatobiliary disease. Additionally, normal reference ranges of CBD to aorta ratio (CBD: Ao ratio) were established which is not affected by BW.MethodsCBD diameter was measured at three different sites: porta hepatis (PH), duodenal papilla (DP) level and mid-portion (Mid) between these points using computed tomography (CT) in 283 dogs without hepatobiliary disease.ResultsThe reference range of CBD diameter at PH level: 1.69 ± 0.29 mm (Class 1; 1 kg ≤ BW &lt; 5 kg), 1.92 ± 0.35 mm (Class 2; 5 kg ≤ BW &lt; 10 kg), 2.20 ± 0.43 mm (Class 3; 10 kg ≤ BW &lt; 15 kg), 2.79 ± 0.49 mm (Class 4; 15 kg ≤ BW &lt; 30 kg); Mid-level: 2.06 ± 0.25 mm (Class 1), 2.43 ± 0.37 mm (Class 2), 2.74 ± 0.52 mm (Class 3), 3.14 ± 0.44 mm (Class 4); DP level: 2.33 ± 0.34 mm (Class 1), 2.90 ± 0.36 mm (Class 2), 3.35 ± 0.49 mm (Class 3), and 3.83 ± 0.50 mm (Class 4). There was a significant difference in CBD diameter at each level among all BW groups. Furthermore, BW and CBD diameter showed positive linear correlation at each level. We devised CBD: Ao ratio at each level that showed no significant difference between the different BW groups; PH level: 0.34 ± 0.05; Mid-level: 0.42 ± 0.06; DP level: 0.47 ± 0.06.ConclusionIn conclusion, since the CBD diameter for each BW is significantly different, different normal reference ranges of CBD diameter should be applied for each BW, and the CBD: Ao ratio can be used regardless of the BW.
Análise dos temas de estudos científicos produzidos no curso de aperfeiçoamento de oficiais e curso superior de polícia: contribuições e efeitos sobre o sistema de governança na Polícia Militar do Paraná
Authors: Perovano Dalton Gean 
Published: March 2023

O estudo apresenta a análise dos temas de pesquisas científicas elaborados no Curso de Aperfeiçoamento de Oficiais (CAO) e Curso Superior de Polícia (CSP), no período de cinco anos (2017 a 2021). A investigação tem por objetivo estudar as contribuições da pesquisa e seus efeitos sobre o sistema de governança da Polícia Militar do Paraná (PMPR), a partir do rol de estudos realizados pelo CAO e CSP, além de detalhar as normas que mantém relação com a educação e a pesquisa científica, na promoção e incremento das políticas públicas internas e externas à Corporação. Para a análise dos dados de pesquisa foi adotado o enfoque misto (qualitativo e quantitativo) e tipo de estudo descritivo. Os dados foram fornecidos pelo Centro de Pesquisa e Pós-Graduação da Academia Policial Militar do Guatupê (CPPG/APMG). Dentre os principais resultados obtidos na investigação destaca-se a escassez de periódicos científicos nos centros de pesquisa em segurança pública. A legislação federal trata sobre o fomento e investimento para a pesquisa científica, mas não tem a previsão clara de captação e a destinação de recursos financeiros para a segurança pública. Os temas abordados nas pesquisas de CAO e CSP contribuem para o cenário de desenvolvimento da Corporação, contudo, necessita maior abrangência na abordagem dos assuntos, a fim de alcançar a amplitude dos fenômenos sociais e Institucionais.
Current problems of legislative regulation of migration processes in the territory of the CIS member states
Authors: Avrutin Roman , Bezhencev Aleksandr 
Published: March 2023

On October 14, 2022 at St. Petersburg University of the Ministry of Internal Affairs of Russia within the framework of the scientific school «Administrative and administrative-procedural activity of the Ministry of Internal Affairs of Russia» the annual International scientific-practical conference «Actual problems of legislative regulation of migration processes in the CIS member states» was held, where opinions on problematic issues of legislative regulation of migration processes, administrative-jurisdictional activity of internal affairs bodies of Russia were exchanged. The conference was attended by representatives of the Ministry of Internal Affairs of foreign countries: Republic of Belarus, Republic of Kazakhstan, Republic of Uzbekistan, Republic of Tajikistan, Republic of Angola, Republic of Ghana, Republic of Guinea-Bissau, Republic of Congo, Republic of Nicaragua, Republic of El Salvador, State of Saint Vincent and the Grenadines.
The conference participants were of the same opinion on the need to strengthen both cooperation between practitioners of the internal affairs bodies of the Ministry of Internal Affairs of Russia, state and other organisations working in the field of migration, and cooperation at the level of CIS member states, and states whose representatives were taking part in the conference.
Niedostrzegane skutki fundacji kompleksu kalwaryjnego Mikołaja Zebrzydowskiego
Authors: Płonka Mirosław 
Published: March 2023

Celem autora było udowodnienie, że kalwaria Mikołaja Zebrzydowskiego nie była (jak dotychczas twierdzono) wzniesiona na terenie pustostanu. Skopiowana na ziemiach pomiędzy Górą Żar a Górą Lanckorońską za traktatem Christiana Kruik van Adrichema Jerozolima obejmowała przede wszystkim królewszczyzny, głównie wsie Zakrzów, Stronie i Leśnicę. Nałożenie układu Miasta Świętego na tereny wiejskie spowodowało zabranie chłopom najbardziej urodzajnych pól znajdujących się na południowych stokach, a w konsekwencji m.in. głód i migrację. W związku z tym autor rozpatrzył fundację kompleksu jako powód zmian układów ruralistycznych i przemieszczeń ludności chłopskiej. W tym celu zostały przeanalizowane odpisy z nieistniejących ksiąg kancelarii kościelnych, rzucające nowe światło na fundację polskiej Jerozolimy. Zawarte w nich wiadomości, uzupełnione informacjami ze źródeł Archiwum Głównego Akt Dawnych w Warszawie, Archiwum Kurii Metropolitalnej i Archiwum Prowincji Bernardynów w Krakowie oraz z wydanymi drukiem materiałami dały spójny obraz sytuacji tenuty zakrzowskiej w XVII wieku. Już w pierwszej połowie tego stulecia nastąpił niemal całkowity upadek wsi Leśnica i zmiany organizacji wsi Stronie, którą końcem XVII wieku starano się ratować zakładaniem nowego osiedla na wzgórzu Niespiennik. Na pozostałych w rękach chłopów kamienistych polach, położonych na północnych stokach, rósł tylko owies. Poza tym spustoszenie przynosiły wylewy rzeki Stryszówki. Zaprowadzona przez Zebrzydowskiego fundacja odcisnęła się także na wiejskiej parafii Stryszów, której proboszcz zaczął od początku XVII stulecia notować spadek liczby przychodzących do stryszowskiego kościoła parafian i wydawanego mu z obu wsi mesznego.
ANALISIS KEJADIAN UPCODING BIAYA PELAYANAN KESEHATAN DI WILAYAH KERJA BPJS KESEHATAN CABANG DEPOK
Authors: Sariunita Nadira , Syakurah Rizma Adlia 
Published: March 2023

Banyak jenis kecurangan (fraud) yang bisa terjadi di pelayanan kesehatan BPJS Kesehatan. Menurut laporan Staf Utilisasi Pelayanan Kesehatan Rujukan dan Anti Fraud BPJS Kesehatan KC Depok, diketehui bahwa kejadian Upcoding pelayanan kesehatan merupakan salah satu permasalahan yang sering ditemukan dalam kecurangan (fraud) di BPJS Kesehatan KC Depok. Studi ini bertujuan untuk menganalisis faktor kejadian upcoding pelayanan kesehatan di wilayah kerja BPJS Kesehatan Kantor Cabang Depok. Sebuah desain kualitatif deskriptif dengan pendekatan studi kasus digunakan dalam artikel ini. Kegiatan dilaksanakan pada tanggal 1 Juli sampai 30 Juli 2021 di Bidang Penjaminan Manfaat Rujukan (PMR) BPJS Kesehatan Kantor Cabang Depok. Pengumpulan data dilakukan dengan observasi, wawancara, dan telaah dokumen. Data yang dikumpulkan dianalisis secara deskriptif. Faktor kejadian upcoding yaitu sumber daya manusia yang kurang berkompeten, adanya kesalahan persepsi diagnosis oleh koder, jumlah berkas yang menumpuk, dan kecendrungan penambahan biaya klaim. Kejadian Upcoding menimbulkan dampak bagi BPJS Kesehatan Kantor Cabang Depok yaitu meningkatkan biaya klaim, meningkatkan beban biaya pelayanan kesehatan yang di tanggung BPJS Kesehatan dan ketidaksesuaian data statistik dengan gambaran kasus di lapangan. Disarankan bagi BPJS Kesehatan Kantor Cabang Depok melakukan pertemuan rutin antara kedua belah pihak dalam rangka motivasi kader agar tidak melakukan kesalahan dalam proses pengkodingan klaim. Bagi Pihak Rumah Sakit terus melakukan pengendalian internal.
النَّقد البيئيّ أفقٌ أخضر في الدِّراسات النَّقديّة المعاصرة
Authors: السُّلطانيّ إيمان 
Published: March 2023

إنَّ البحث يُقدِّم فرعاً نقديّاً جديداً في الدِّراسات النِّقديّة المعاصرة ، وهو: (النَّقد البيئيّ) ، بصفته فرعاً نقديّاً يرمي إلى قراءة النُّصوص الأدبيّة الخاصَّة بالبيئة وتمثُّلاتها الطَّبيعيّة ، والمشاكل التي سعى الأدب إلى تناولها ، ومحاولة معالجتها ، وتحليل تلك النُّصوص ، وكشف وجهات النَّظر التي تحملها ، وبيان قيمتها الجماليّة والأخلاقيّة في آنٍ معاً ، من ثمَّ إيجاد الحلول النَّاجعة لحلِّ أزمة البيئة من نواحٍ مختلفةٍ ، وقد بُني البحث على ثلاثة محاور رئيسة:
المحور الأَوَّل: وسم بـ: (البيئة ، وأُصولها في العلوم ، والدِّراسات) .
المحور الثَّاني: وسم بـ: (قراءة في الأدب البيئيّ ، وأُصوله) .
المحور الثَّالث: وسم بـ: (النَّقد البيئيّ ، وآفاقه الجديدة في النَّقد المعاصر) .