bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low Expression of PRRG2 in Kidney Renal Clear Cell Carcinoma: An Immune Infiltration-Associated Prognostic Biomarker
Authors: Tang Gonglin , Ding Guixin , Liu Shangjing , Liu Hongquan , Wang Tianqi , Zou Qingsong , Sun Kai , Wu Jitao 
Published: August 2023

Abstract
Background:
Kidney renal clear cell carcinoma (KIRC) is a highly prevalent and lethal type of cancer, and one of the primary approaches to address it is the development of suitable biomarkers. The objective of this study is to investigate the prognostic value of proline-rich γ-carboxyglutamic acid protein 2 (PRRG2) in KIRC and its correlation with immune cell infiltration.
Methods:
The study comprehensively clarified the prognostic value and potential function of PRRG2 in KIRC. PRRG2 expression was analyzed and correlated with sex, age, metastasis, and pathological stage in KIRC patients. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) were used to explore the involvement of PRRG2 in the immune response. The correlation between PRRG2 expression and immune cell infiltration was also analyzed. Finally, the confirmation of cellular and tissue identity was achieved through the utilization of immunohistochemical staining and quantitative real-time PCR.
Results:
The study reveals a significant reduction in PRRG2 expression among KIRC patients. Low PRRG2 expression is associated with gender, age, metastasis, and pathological staging, and significantly predicts poor prognosis in KIRC patients. GO, KEGG, and GSEA results indicate the involvement of PRRG2 in immune response. PRRG2 expression is significantly correlated with the infiltration level of immune cells and the expression of different immune cell markers.
Conclusion:
PRRG2 may potentially impact prognosis in KIRC patients by regulating immune infiltration, thus rendering PRRG2 a promising candidate prognostic biomarker for KIRC-associated immune infiltration.
Reliability and validity of a single-item measure of recovery expectations in rehabilitation research and practice
Authors: Carrière Junie , Berbiche Djamal , Montemurro Laury , Sullivan Michael 
Published: August 2023

Abstract

Purpose
The objective of this study is to investigate the reliability and validity of a single-item measure of recovery expectations in the context of work disability.
Methods
Two independent samples of individuals with musculoskeletal pain conditions were used to evaluate the reliability and validity of a single-item measure of recovery expectations (“How likely is it that you will return to work?”). Sample 1 consisted of 109 individuals with work-related musculoskeletal injuries and Sample 2 consisted of 152 individuals with whiplash injury. Participants completed measures of demographics, recovery expectations and pain-related psychological variables (depressive symptoms, catastrophizing, fear of movement and perceptions of injustice). At 1-year follow-up, participants reported their work status. Analyses examined the test-retest reliability, parallel-form reliability, construct validity and predictive validity of a single-item measure of recovery expectations on work status at 1-year follow-up.
Conclusion
Single-item measures of recovery expectations are a reliable and valid tool to predict work disability at 1-year follow-up in individuals with musculoskeletal pain conditions. This study represents a step towards practice-based evidence by providing a brief, low burden, low-cost measure of recovery expectations that can be seamlessly integrated into clinical workflow and research protocols.
Challenges of Diagnosing Severe Malaria with Complications in Adult Patients: A Case Report
Authors: Bur Rika , Nelwan Erni Juwita , Danasasmita Ira , Hakim Gardian Lukman , Bahri Syukrini , Dewi Febby Elvanesa Sandra , Athaya Rana Zara , Nainggolan Leonard 
Published: August 2023

Abstract
Background: Malaria is known to be the main cause of death in malaria-endemic areas. The authors report a case of severe malaria in an adult with no history of travel from an endemic area with good outcomes after hospitalization.
Case Presentation: A 46-year-old man was brought to the Emergency Room (ER) because of fever and chills for six days. Complaints were accompanied by nausea and vomiting three times a day. Patients also experience headaches, weakness, coughing, and runny nose. The patient had no history of traveling from a malaria-endemic area. The patient was transferred from the Emergency Department (ED) to the High Care Unit (HCU), and during one day of intensive medical care at the HCU, there was a clinical deterioration characterized by dyspnea, icteric sclerae, acral edema, tenderness in both calves, and rash in the abdominal area. Due to worsening respiratory function, the patient was placed on a ventilator. During intensive medical treatment, the patient continued to show deterioration. The clinical findings suggest a possible feature of Weil's disease or fulminant hepatitis, and although the patient was on intensive care, there was no clinically significant improvement. Furthermore, microscopic blood smear examination and rapid diagnostic tests (RDTs) for malaria were carried out on the 4th day of treatment with negative results. As there was no clinically significant improvement, it was decided to take a blood smear and repeat RDT on the twelfth day, and it showed a positive result for falciparum malaria. Subsequently, artesunate was administered intravenously, and the patient's condition began to improve with a negative parasite count the following day. The patient was discharged in good clinical condition on day 25 of treatment.
Conclusion: Good quality malaria diagnostic techniques are essential to diagnose malaria. A timely diagnosis of malaria has the potential to save the patient. Because Jakarta is not a malaria endemic area, it was concluded that this case was an imported malaria case.
Association between aspirin dose and outcomes in patients with acute Kawasaki disease: A nationwide retrospective cohort study in Japan
Authors: Suzuki Takanori , Michihata Nobuaki , Hashimoto Yohei , Yoshikawa Tetsushi , Saito Kazuyoshi , Matsui Hiroki , Fushimi Kiyohide , Yasunaga Hideo 
Published: August 2023

AbstractBackground Using a Japanese national inpatient database, this study aimed to identify the appropriate dose of aspirin to be prescribed to patients with acute Kawasaki disease. Method We identified patients with Kawasaki disease treated with intravenous immunoglobulin between 2010 and 2021.The outcomes included the occurrence of coronary artery abnormalities and intravenous immunoglobulin resistance, length of stay, and medical costs. Restricted cubic spline functions were performed to examine the association between aspirin dose and the outcomes. Results Data of 82109 patients were extracted from the database. Non-linear associations were observed between aspirin dose and the outcomes. In comparison with an aspirin dose of 30 mg/kg/day, the odds ratio (95% confidence interval) for coronary artery abnormalities was 1.40 (1.13–1.75) at 5 mg/kg/day. An aspirin dose of ≥ 30 mg/kg/day did not significantly change the odds ratio for coronary artery abnormalities. Intravenous immunoglobulin resistance was significantly lower at a dose of 60 mg/kg/day or over. Conclusions The results showed no significant association between aspirin escalation and CAAs in patients with acute KD. High-dose aspirin showed the potential to reduce hospital stay and medical costs without increasing complications.
Bacteria inhabiting spider webs enhance host silk extensibility
Authors: Tsiareshyna Maryia , Wang Te-Hsin , Lin Yin-Sheng , Piorkowski Dakota , Huang Sammi Yen-Ting , Huang Yi-Lun , Chao Wei-Ting , Chang Yuan-Jay , Liao Chen-Pan , Wang Pi-Han , Tso I-Min 
Published: August 2023

Abstract

Spider silk is a promising material with great potential in biomedical applications due to its incredible mechanical properties and resistance to bacterial degradation, particularly commercially available strains. However, little is known about the bacterial communities that may inhabit spider webs and how these microorganisms interact with spider silk. In this study, we exposed two exopolysaccharide-secreting bacteria, isolated from webs of an orb spider, to major ampullate (MA) silk from host spiders. The naturally occurring lipid and glycoprotein surface layers of MA silk were experimentally removed to further probe the interaction between bacteria and silk. Extensibility of major ampullate silk produced byTriconephila clavatathat was exposed to eitherMicrobacteriumsp. orNovosphigobiumsp. was significantly higher than that of silk that was not exposed to bacteria. This strain-enhancing effect was not observed when the lipid and glycoprotein surface layers of MA silks were removed. The presence of exopolysaccharides was detected through NMR from MA silks exposed to these two bacteria but not from those without exposure. Here we report for the first time that exopolysaccharide-secreting bacteria inhabiting spider webs can enhance extensibility of host MA silks and silk surface layers play a vital role in mediating such effects.
Finite element analysis of convective heat transfer in a linearly heated porous trapezoidal cavity in the presence of a magnetic field
Authors: Suchana Sanzina Sultana , Ali Mohammad Mokaddes 
Published: August 2023

Abstract
A computational study is carried out to examine how a magnetic field affects convective heat transfer in a linearly heated trapezoidal cavity, filled with a fluid-saturated by porous medium. Both inclined walls are adiabatic, with the top wall maintaining a constant cooled temperature Tc which is moving with a constant velocity U0 in the positive x-axis direction, and the bottom wall being heated linearly which is moving with a constant velocity that is the same as that of the top wall but in the opposite direction. The finite element technique is utilized to resolve the governing equations associated with appropriate initial and boundary conditions. The resulting solutions are represented graphically in respect of streamlines, isothermal lines, and thermal gradient magnitude for an extensive range of Darcy numbers (10− 2 ≤ Da ≤ 10− 5), Hartmann numbers (0 ≤ Ha ≤ 100), and Prandtl numbers (0.7 ≤ Pr ≤ 15). The findings indicate the enhancement of flow circulation concerning the higher values of Darcy number but the reduction of that with the higher values of Hartmann number as well as Prandtl number. In addition, the temperature distribution is affected by various values of the Darcy number and Hartmann number. It is also reflected that the rate of convective heat transfer declines with the growing values of the Hartmann number and the decreasing values of the Darcy number and Prandtl number.
Evaluation of plateau wetland ecological security and its influencing factors in multi-climatic zones: A case study of Yunnan Province
Authors: Chen Zhe , Zhang Chunhong , Chen Zhe 
Published: August 2023

Abstract
Plateau wetlands play an important role in social and economic development and ecological security. The ecological environment of a plateau wetland is fragile and vulnerable to external interference, resulting in changes in the wetland structure and ecological functions. Ecological security assessment of plateau wetlands is the key to fully understanding their ecological security situation. Yunnan Province, which is located in the Qinghai-Tibet Plateau, has a significant stereoscopic climate, which is characterized by distinct features of diverse and complex wetlands. Thus, studying the wetland ecological security of Yunnan Province spanning various climatic zones is of great significance. In this study, an improved pressure-state-response model was constructed, and an index system containing state and social pressure was established using remote sensing data, Yunnan Province Yearbook data, and socioeconomic statistical data. Taking 16 prefectures in Yunnan Province as samples, this study weighted the evaluation indices via the combination of entropy weighting and expert weighting. In addition, the wetland ecological security state in Yunnan Province in 2017 was evaluated using the fuzzy matter-element method, and the influencing factors were analyzed. Results showed that the highest ecological security index of wetland was distributed in Nujiang, Diqing in the northwest of Yunnan Province and Xishuangbanna in the south, whereas the lowest area was in Qujing, Zhaotong in the northeast of Yunnan Province and Baoshan in the west. Differences were observed in the influencing factors of wetland ecological security in different prefectures. In general, the influencing factors in Yunnan Province were the annual average temperature, proportion of natural wetland, percentage of secondary industry, annual precipitation, wetland ecological service function per unit acreage, fertilization intensity, and industrial water recycling rate. The results of grey correlation analysis showed that climatic factors and human disturbance activities were the main influencing factors of wetland ecological security in Yunnan Province, and the influence rate of the two was close to each other. The findings of this work could provide suggestions for the decision making on ecological security development in plateau wetlands across multi-climate zones, as well as data support for the ecological protection of plateau wetlands.
The synergistic effect of EGCG and conditioned medium derived from WJ-MSCs on androgen receptor and apoptotic signaling pathways of prostate cancer LNCaP cells
Authors: Mohammadi Reihane , Fazeli Zahra , Daraei Azam , Asgari Katayoon 
Published: August 2023

Abstract
Prostate cancer (PCa) was known as the second most common cancer in men. Although there were several approaches to treat this disease, the cost and side effects of some approaches have encountered the patients with challenges. Therefore, the design of new therapy methods could be useful in the management of this disease. For this purpose, the synergic effect of Epigallocatechin gallate (EGCG) and conditioned medium derived from Wharton's jelly mesenchymal stem cells (WJ-MSCs) were studied on prostate cancer LNCaP cells. In this study, LNCaP cells were treated with different concentrations of EGCG and conditioned medium derived from WJ-MSCs (WJCM). The viability of treated cells was determined by using MTT assay. Then, the expression of androgen receptor (AR and PSA) and apoptotic (BAX, CASP3 and CASP7) pathway genes were defined by Real time PCR. The analysis of the data indicated that the treatment with 400µM EGCG in combination with 50% WJCM (0% FBS) for 72 hours decreased expression of AR and PSA genes as well as the enhanced expression of BAX, CASP3 and CASP7 genes in the LNCaP cells (p < 0.05). The obtained results suggested that the combination therapy of EGCG and WJCM had an anticancer effect on LNCaP cells through activation of apoptotic pathway and suppression of androgen receptor pathway.
Effect of Solid-Electrolyte Pellet Density on Failure of Solid-State Batteries
Authors: Ceder Gerbrand , Diallo Mouhamad , Shi Tan , Zhang Ya-Qian , Peng Xinxing , Shozib Imtiaz , Wang Yan , Miara Lincoln , Scott Mary , Tu Howard 
Published: August 2023

Abstract
Despite the potentially higher energy density and improved safety of solid-state batteries (SSBs) relative to Li-ion batteries, failure due to Li-filament penetration of the solid electrolyte and subsequent short circuit remains a critical issue. Herein, we show that Li-filament growth is suppressed in solid-electrolyte pellets with a relative density beyond ~ 95%. Below this threshold value, however, the battery shorts more easily as the density increases due to faster Li-filament growth within the percolating pores in the pellet. The microstructural properties (e.g., pore size, connectivity, porosity, and tortuosity) of 75%Li2S - 25P2S5 with various relative densities are quantified using focused ion beam–scanning electron microscopy tomography and permeability tests. Furthermore, modeling results provide details on the Li-filament growth inside pores ranging from 0.2 to 2 µm in size. Our findings improve the understanding of the failure modes of SSBs and provide guidelines for the design of dendrite-free SSBs.
The wave mechanics for microwave absorption film-Part 3: Film with multilayers
Authors: Liu Yue , Liu Ying , Drew Michael 
Published: August 2023

Abstract

In the field of microwave absorption, the quarter wavelength theory (QWM) is usually applied to film with multiple layers. However, it is demonstrated here that this is inappropriate since QWM is a flawed theory. The results for the conventional film with or without metal-back are usually applied to the middle layers in multilayer film. But this is inappropriate since there are incident microwaves at both sides of the middle layer but only the incident microwaves in front of the film are considered. No suitable theoretical treatment for multilayer film has been developed so far and this has been done in this work.
The effect of soil porosity size distribution on integral energy content of water in different moisture slopes
Authors: Dai Liuben , Xu Zhaolei 
Published: August 2023

Abstract
The water required by the plant is provided through the porous environment of the soil around the root. Therefore, indicators such as soil texture and effective porosity have been defined to measure the amount of water available to the plant. The pore size determines water energy and the plant's ability to absorb moisture, which is evaluated in this article. Multivariate regression analysis between the integral energy of water in different ranges of humidity as the dependent variable and the indices of soil pore size distribution as the independent variable has been investigated. Due to the fact that none of the indices of the pore size distribution curve have a significant relationship with the integral energy value of water in the permanent wilting point, therefore, it was not possible to estimate this index using the indices of the pore size distribution curve. The results showed that the elongation index of soil pore size distribution curve is the only factor determining the amount of integral energy of water in different soil water contents.
relação entre a satisfação de ensinar e o método das Boquinhas®
Authors: Rangel Alexandra Fernandes Morais , Jardini Renata Savastano Ribeiro , Bongiovani Anair , Tosta Estela Inês Leite 
Published: August 2023

A alfabetização gera impactos em toda a vida do indivíduo, sendo um momento em que a criança é inserida na sociedade, tornando-se parte desse grupo. Insucessos nessa aquisição podem acarretar consequências emocionais para todos os envolvidos, educadores e aprendizes. Os objetivos dessa pesquisa foram investigar o grau de satisfação, motivação, autoestima, envolvimento profissional e facilidade dos professores/clínicos, que aplicam o Método das Boquinhas®[1] no processo de alfabetização e reabilitação, bem como sua apreciação sobre os mesmos sentimentos em relação ao seu aprendiz. Trata-se de uma pesquisa de natureza quantitativa, na qual foram aplicados dois questionários, e os resultados obtidos apontaram altos índices de confiabilidade em todos os itens pesquisados, tanto na percepção do educador, como de seu educando. Também houve alta correlação significativa, principalmente na maior quantidade de cursos realizados e tempo de estudos em relação à aprendizagem e recomendação da metodologia. Concluiu-se, portanto, que o educador sente benefícios para si e para seus aprendentes ao aplicar a metodologia Boquinhas.

[1] Método de alfabetização multissensorial, fonovisuoarticulatório, registrado por Renata Jardini, em 2008, com INPI para produtos e serviços. Disponível em: www.metododasboquinhas.com.br. Acesso em 10: dez. 2022.
Discovery Phenolic Profiles and in vitro Antioxidants, Neuroprotective,
Anti-diabetic Activities of Extracts from of Algerian Plant: Calendula
monardii Boiss. & Reut
Authors: Samai Zakaria , Toudert Nadia , Dadda Noureddine , Hamel Tarek , Zakkad Farida , Zerrad Chaima , Boutemedjet Sihem , Bensouici Chawki , Djilani Salah Eddine 
Published: August 2023

Abstract:
The present study examines the potential effect of Calendula monardii (Boiss. & Reut.) on
inhibiting various enzymes involved in many diseases.

Background:
Calendula suffruticosa subsp. monardii (Boiss. & Reut.) Ohle, a medicinal plant from the
Mediterranean region and, more precisely, from the extreme northeast of Algeria, is characterized by its
wide use in the traditional medicine of the local population. This is what prompted us to investigate
some pharmacological benefits such as anti-diabetes and Alzheimer's activities, in addition to antioxidant
activity.
The effects of extraction methods and solvents on the amount of phenolic profiles and the biological activity
of the different parts of this plant were studied, where the aim was to obtain a high extraction
yield of bioactive compounds and consequently high biological activities.

Methods:
In vitro standard procedures were used to assess enzyme inhibitory activity (AChE, BChE, α-
Amylase and α-Glucosidase) of Calendula monadii, and the antioxidant activity was assessed using the
DPPH, ABTS, CUPRAC, Reducing power and Ferrous ions assays.

Results:
When using ultrasound, a significant increase in the amounts of (TPC, TFC and TFlas) and antioxidant
activity (DPPH, ABTS, CUPRAC, Reducing power and Ferrous ions cheating assay) in addition
to the inhibitory activity of enzymes (AChE, BChE, α-Amylase α-Glucosidase) was found, compared
to the results of conventional extraction. Furthermore, the aqueous solvent of ethanol 70% is the
very effective solvent for extraction compared to methanol 70% aqueous solvent.

Conclusion:
Based on these results, it can be said that this plant contains important biological activities,
so it can be used in phytotherapy.
Expression of MicroRNA-200a/b/c in the Mediobasal Hypothalamic Nuclei with Aging
Authors: Porseva Valentina V. , Pankrasheva Lydia G. , Moiseev Konstantin Yu , Anfimova Polina A. , Emanuilov Andrey I. , Levshin Nikolay Yu. , Baranov Andrey A. , Masliukov Petr M. 
Published: August 2023

Background:
MicroRNAs (miRNAs) belong to small non-coding RNAs that coordi-nate the expression of cellular genes at the post-transcriptional level. The hypothalamus is a key regulator of homeostasis, biological rhythms and adaptation to different environmental factors. It also participates in the aging regulation. Variations in miRNA expression in the hypothalamus can affect the aging process.

Objective:
Our objective of this study is to examine the expression of miR-200a-3p, miR-200b-3p, miR-200c-3p in the dorsomedial (DMN), ventromedial (VMN) and arcuate (ARN) nuclei of the hypothalamus in male and female rats during aging.

Methods:
The expression of miR-200a-3p, miR-200b-3p, and miR-200c-3p in DMN, VMN and ARN was studied by qPCR-RT. The results were presented using the 2-ΔΔCq algorithm.

Results:
The expression of miR-200a-3p, miR-200b-3p, miR-200c-3p microRNAs decreases with aging in the DMN of males and in the VMN of females. The level of miR-200b-3p expression de-creased in aged males in the VMN and females in the DMN. The expression of miR-200c-3p de-clined in aged males in the ARN and in females in the DMN. The expression of miR-200a-3p, miR-200b-3p, and miR-200c-3p did not change in females in the ARN in aging.

Conclusion:
We found a decrease in the expression of members of the miR-200a-3p, miR-200b-3p, and miR-200c-3p in the tuberal hypothalamic nuclei and their sex differences in aging rats.