bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coordinated modulation of long non-coding RNA ASBEL and curcumin co-delivery through multicomponent nanocomplexes for synchronous triple-negative breast cancer theranostics
Authors: He Xuesong , Lin Fengjuan , Jia Runqing , Xia Yang , Liang Zhaoyuan , Xiao Xiangqian , Hu Qin , Deng Xiongwei , Li Qun , Sheng Wang 
Published: August 2023

Abstract
Background
Abnormally resgulated long non-coding RNAs (lncRNAs) functions in cancer emphasize their potential to serve as potential targets for cancer therapeutic intervention. LncRNA ASBEL has been identified as oncogene and an anti-sense transcript of tumor-suppressor gene of BTG3 in triple-negative breast cancer (TNBC).
Results
Herein, multicomponent self-assembled polyelectrolyte nanocomplexes (CANPs) based on the polyelectrolytes of bioactive hyaluronic acid (HA) and chitosan hydrochloride (CS) were designed and prepared for the collaborative modulation of oncogenic lncRNA ASBEL with antago3, an oligonucleotide antagonist targeting lncRNA ASBEL and hydrophobic curcumin (Cur) co-delivery for synergetic TNBC therapy. Antago3 and Cur co-incorporated CANPs were achieved via a one-step assembling strategy with the cooperation of noncovalent electrostatic interactions, hydrogen-bonding, and hydrophobic interactions. Moreover, the multicomponent assembled CANPs were ulteriorly decorated with a near-infrared fluorescence (NIRF) Cy-5.5 dye (FCANPs) for synchronous NIRF imaging and therapy monitoring performance. Resultantly, MDA-MB-231 cells proliferation, migration, and invasion were efficiently inhibited, and the highest apoptosis ratio was induced by FCANPs with coordination patterns. At the molecular level, effective regulation of lncRNA ASBEL/BTG3 and synchronous regulation of Bcl-2 and c-Met pathways could be observed.
Conclusion
As expected, systemic administration of FCANPs resulted in targeted and preferential accumulation of near-infrared fluorescence fluorescence signal and Cur in the tumor tissue. More attractively, systemic FCANPs-mediated collaborative modulating lncRNA ASBEL/BTG3 and Cur co-delivery significantly suppressed the MDA-MB-231 xenograft tumor growth, inhibited metastasis and extended survival rate with negligible systemic toxicity. Our present study represented an effective approach to developing a promising theranostic platform for combating TNBC in a combined therapy pattern.
The microbiome of the branching coral Acropora kenti
Authors: Messer Lauren , Bourne David , Robbins Steven , Clay Megan , Bell Sara , McIlroy Simon , Tyson Gene 
Published: August 2023

Abstract
Microbial diversity has been extensively explored in reef-building corals however the functional roles of coral-associated microorganisms remain poorly elucidated. Here, metagenome-assembled genomes (MAGs) were recovered from the coral Acropora kenti (formerly A. tenuis) to elucidate microbial functions and metabolic interactions within the holobiont. Overall, 191 bacterial and 10 archaeal MAGs assigned to 16 phyla were recovered from A. kenti and the adjacent seawater. Based on their prevalence and relative abundances, 82 of these MAGs were specific to the A. kenti holobiont, including members of the Proteobacteria, Bacteroidota, and Desulfobacterota. A. kenti-specific MAGs displayed significant differences in their genomic features and functional potential relative to seawater specific-MAGs, having a higher prevalence of genes involved in host immune system evasion, nitrogen and carbon fixation, and synthesis of five essential B-vitamins. Moreover, a diversity of A. kenti-specific MAGs encoded genes required for the synthesis of essential amino acids, such as tryptophan, histidine, and lysine, which cannot be de novo synthesised by the host or Symbiodiniaceae. Across a water quality gradient spanning 2° of latitude, A. kenti microbial community composition correlated to increased temperature and dissolved inorganic nitrogen. Corresponding enrichment in molecular chaperones, nitrate reductases, and a heat-shock protein, indicated phenotypic adaptation of the microbiome to localised anthropogenic pressures. These findings reveal mechanisms of A. kenti-microbiome-symbiosis on the Great Barrier Reef, highlighting the interactions underpinning the health and functioning of this keystone holobiont.
Bio-active principles in Commiphora africana resin dichloromethane extract and their insecticidal activity against bedbugs
Authors: Wairagu Norman W. , Wachira Benson M. , Githiomi Joseph K. , Oduor Nellie , Ng’ang'a Margaret M. 
Published: August 2023

Abstract
Bedbugs (Cimex lecturalius Linnaeus) are ecto-parasite pests that wholly feed on human and domestic animals’ blood causing anemia to the host on excessive feeding. Bedbug control has proved futile due to various challenges including; development of insecticide resistance, high associated cost and environmental pollution. Natural herbal-based phytochemicals remain unexploited and we focused on Comiphora africana (A. Rich.) Engl. resin traditsionally used bedbug control. We previously showed that dichloromethane extract of C. africana resin is highly repellent and toxic against bedbugs. In this study, we isolated compounds from the dichloromethane extract using column chromatographic techniques. The isolated compounds were evaluated for repellency and toxicity against bedbugs; and characterized using conventional spectroscopic techniques. Five compounds (taraxasterol, pseudo-taraxasterol, beta-sitosterol, fungisterol and guggusterol) were isolated and characterized for the first time in this plant. Fungisterol had the highest repellency (75%) against bedbugs which was not significantly different (P > .05) to the positive control (neocidol) (74%) after > one-hour exposure. Fungisterol also elicited highest toxicity against bedbugs with LC50 of 25.73 mg/L after 24 h exposure. Blending fungisterol with other identified active terpenes did not synergize the overall repellent/toxic responses. This study identifies active compounds in C. africana resin and therefore lays a solid background in bedbug control using isolated compounds.
The intracellular domain of Hyaluronan synthase can produce monodisperse low- molecular weight hyaluronic acid with functional activity
Authors: Ebrahimi Tahereh , Keramati Malihe , Khodabakhsh Farnaz , Cohan Reza Ahangari 
Published: August 2023

Abstract
Background:
The biological properties of hyaluronan (HA) are determined by its molecular weight (MW). Most natural extraction process of HA resulted in a polydisperse polymer. In the present study, the monodisperse LMW-HA was biosynthesized by truncated recombinant hyaluronan synthase (HAS) derived from Streptococcus equisimilis (SeHAS). The effects of t transmembrane domains truncation of SeHAS on productivity and specific activity of enzymes, dispersity, MW, and biological activity of synthesized polymers were investigated. The recombinant HAS123 (341 aa), HAS23 (309 aa), and HASIntra (263 aa) were constructed. The MW, structure, and titer of the polymer were studied by electrophoresis, FTIR spectroscopy and carbazole assay, respectively. The biological activity of HA polymers was assessed by cell proliferation and wound healing assays.
Results:
The specific activities were calculated as 7.5, 6.8, 4.9, and 2.8 mgHA.mgprotein-1.min-1 for SeHAS, HAS123, HAS23, and HASIntra, respectively. The results revealed SeHAS produced a polydispersed HMW-polymer (268 kDa), while HAS123 and HAS23 produced a polydisperse LMW-polymer (6.1, and 4.6 kDa, respectively). Interestingly, HASIntra produced a monodisperse LMW-HA (1.9 kDa). Biological assessments indicated the LMW-HAs showed a dose-dependent proliferation activity on endothelial cells (ECs), whereas HMW-HAs exhibited an inhibitory effect. Also, LMW-HAs had the highest wound healing effect at 10 µg/mL, while by increasing in the concentration to 200 µg/mL, both LMW- and HMW-HAs postponed the wound healing recovery rate.
Conclusion:
The study indicated the transmembrane domains of SeHAS affect the titer, size, and dispersity of synthesized HA. These findings open new insight into the rational engineering of SeHAS to produce size-defined HA.
A higher TyG index level is more likely to have enhanced incidence of T2DM and HTN comorbidity in elderly Chinese people: a prospective observational study from the Reaction Study
Authors: Su Wanlu , Wang Jie , Chen Kang , Yan Wenhua , Gao Zhengnan , Tang Xuelei , Wan Qin , Luo Zuojie , Ning Guang , Mu Yiming 
Published: August 2023

Abstract
Background
Triglyceride glucose index (TyG index) was related with both type 2 diabetes (T2DM) and hypertension (HTN). Prospective studies linking TyG index to incidence of T2DM and HTN comorbidity remains unclear. The aim of this study was to explore the longitudinal association between TyG and new-onset T2DM with HTN.
Methods
4,434 subjects (1249 males and 3185 females) without initial T2DM and HTN were followed up for 7 years. The incidence of T2DM with HTN during the 7-year follow-up was identified as the endpoint. The TyG index was divided into four quartiles: the < 25% level, the 25–50% level, the 50–75% level, the ≥ 75% level. The relationships between TyG index and T2DM with HTN were evaluated by Cox proportional hazards regression models.
Results
During 7 years, the augmented trend of T2DM with HTN was observed in the participates. After adjusting for all confounding factors, compared with those in the lowest quartile of TyG index, population in the highest quartile of TyG index had a higher risk of T2DM with HTN (hazard ratio (HR), 2.878; 95% confidence intervals (95% CI), 1.230–6.731, P = 0.015), however, the association remained significant only in the female population (HR 2.753, 95%CI, 1.061–7.139, p = 0.037). The TyG had superior predictive ability of increased risk of T2DM with HTN for the populations of older age (≥ 65 years) (HR 2.694, 95%CI 1.212–5.989, p = 0.015), higher eGFR (≥ 90 mL/ (min·1.73 m2)) (HR 2.603, 95%CI 1.164–5.818, p = 0.020) or obesity (BMI ≥ 28 kg/m2) (HR 2.547,95%CI 1.001–6.478, p = 0.020).
Conclusion
Population with a higher TyG index level was more likely to have enhanced incidence of T2DM and HTN comorbidity. TyG index could have significance of clinical in early protecting against T2DM with HTN.
Associations between leaf developmental stability, canalization and phenotypic plasticity in an architectural perspective
Authors: Wang Shu , Zhou Dao-Wei 
Published: August 2023

Abstract
The associations among developmental stability, canalization and phenotypic plasticity have received increasingly more attention, yet with rare direct evidence. Architectural analysis may provide a more powerful approach to addressing this issue. To investigate the relationships among the three mechanisms in architectural perspective, we subjected plants of Abutilon theophrasti to three densities, measured and calculated fluctuating asymmetry (FA), coefficients of variation (CV) and plasticity (PI) of three leaf traits, to analyze the correlations among these variables. As density increased, mean leaf size, petiole length and angle of most layers and mean leaf FA of some layers decreased (at both stages), CV of petiole angle increased (at day 50), and PI of petiole length and angle across all layers decreased (at day 70); leaf FA and CV of traits generally increased with higher layers at all densities. At both stages, there were more positive correlations between FA and CV at lower vs. high densities; at day 50, little correlation of plasticity with FA or CV was found; at day 70, more positive correlations between FA and PI occurred for response to high vs. low density than for response to medium vs. low density, and more positive correlations between CV and PI occurred at lower vs. high densities. Results suggested that developmental instability, decreased canalization and plasticity can be cooperative and the relationships between decreased canalization and plasticity are more likely to be positive if decreased canalization is due to vibrant growth rather than stressful effects. The relationships of plasticity with developmental instability differed from its relationship with decreased canalization in the way of variation. Decreased canalization should be more beneficial for possible plasticity in the future, while canalization may be the outcome of already-expressed plasticity.
The Influence of the obstacle position on the explosion characteristics of methane-hydrogen-air premixed gas in a closed 90° bend pipe
Authors: Li Xue , Yin Qing , Zhou Ning , Qian Xingyi , Yang Chunhai , Yu Yongbin , Chen Bing , Liu Xuanya , Huang Weiqiu , Yuan Xiongjun , Zhao Huijun 
Published: August 2023

Abstract
Experiment and numerical simulation were combined to reveal the influence of the obstacle position on the combustion and explosion characteristics of methane-hydrogen-air mixture in a closed 90° bend pipe. The results showed that the synergistic effect of the obstacle and the hydrogen addition significantly enhanced the explosion intensity of the mixed gas. Affected by the turbulent vortex near the obstacle, the flame was distorted significantly when it approached the obstacles, thus enhancing the flame instability and accelerating the flame transition from layer to turbulence. The relative position between the obstacle and the elbow in the 90°bend pipe had an obvious influence on the excitation of flame propagation. When the distance between the obstacle and the elbow was more than 6 times the diameter of the pipe, the closer the obstacle was to the ignition end, the stronger the excitation effect. When the distance between the obstacle and the elbow was 2 to 6 times the diameter of the pipe, the excitation effect of the obstacle on the flame propagation gradually increases with the decrease of the distance. When the distance between the obstacle and the elbow was less than twice the diameter of the pipe, because the vortex size was less than the distance between the obstacle and the elbow, the flame easily contacted pipe wall after it entered the vortex area, resulting in partial flame extinguishing,, which was not conducive to the flame acceleration. When the obstacle was located behind the elbow, the excitation effect of the obstacle on the flame propagation was weak. The maximum explosion pressure of the methane-hydrogen-air premixed gas in the barrier bend pipe can be divided into two parts according to obstacle positions. When the distance between the obstacle and the elbow was more than 6 times the diameter of the pipe, the maximum explosion pressure was mainly affected by the obstacle position. However, when the distance between the obstacle and the elbow was less than 6 times the diameter of the pipe, the maximum explosion pressure was comprehensively affected by the combustion characteristics of the mixed gas and the obstacle position.
Random Subspace Evolutionary Feature Selection for High-dimensional Data
Authors: Tran Cao Truong 
Published: August 2023

Abstract
Classification represents a fundamental task in machine learning and data mining. Many real-world datasets exhibit a large number of features but only a limited number of instances, which often poses challenges for conventional classification algorithms. Dealing with such datasets, one prevalent approach involves using feature selection to retain relevant features while eliminating irrelevant ones. Although evolutionary computation algorithms have been extensively used for such problems, they do not perform well with these high-dimensional datasets. To address these limitations, this paper introduces a random subspace evolutionary feature selection method that performs feature selection on small subsets of features to avoid overfitting and speed up the process and employs multiple feature subspaces to prevent stagnation in local optima during feature selection. The experimental results demonstrate that this novel method achieves higher accuracy and greater stability compared to other commonly used benchmark feature selection techniques.
Universal inequalities for eigenvalues of a clamped plate problem of the drifting Laplacian
Authors: He Yue , Pu Shiyun 
Published: August 2023

Abstract
In this paper, we study the universal inequalities for eigenvalues of a clamped plate problem of the driftingLaplacian in several cases, and establish some universal inequalities that are different from those in [Feng Du, Chuanxi Wu, Guanghan Li and Changyu Xia, Z. Angew. Math. Phys. 66 (2015), no. 3, 703-726].
MSC 2010: 35P15, 53C20, 53C42, 58G25.
Bioinformatics analysis of GPS1 expression and biological function in breast cancer
Authors: Wei Hong , Niu Chaocan , Ji Ruixue , Jiang Wenwen , Tang Jiawei , Meng Zhexuan , Cao Xiaoyang , Zhang Xinyi , Liu Xue 
Published: August 2023

Abstract
Backgrounds
G protein pathway suppressor 1 (GPS1) is involved in the development of many diseases including tumors, but its specific regulatory mechanism in breast cancer is not clear. The goal of the present study was to explore the biological effects and underlying mechanism of GPS1 in breast cancer.
Methods
Public databases were used to analyze GPS1 expression and the relationship with clinicopathological characteristics and prognosis of breast cancer patients, combined with in vitro experiments to analyze the mechanism of action and immune relevance of GPS1 in breast cancer.
Results
Data analysis showed that the expression of GPS1 in breast cancer tissues was significantly higher than that in paracancerous tissues (p < 0.001), and the receiver operating curve (ROC) revealed a higher diagnostic efficiency (AUC = 0.832). Survival analyses indicated that patients with high GPS1 expression made the prognosis worse in Luminal B, low to intermediate grade breast cancers. Enrichment analysis showed that GPS1 was involved in the formation of ribonucleoprotein complexes, which dynamically altered the fate of RNA; it could also enhance the responsiveness of the Wnt pathway by interacting with WBP2. In addition, GPS1 expression was closely related to the immune microenvironment. GPS1 knockdown inhibits the proliferation, invasion and migration of MCF7 and MDA-MB-231 cells in vitro.
Conculsions
This study suggests that the upregulation of GPS1 is associated with the malignant biological behavior and prognosis of breast cancer and may promote cancer progression. The correlation between GPS1 and the immune microenvironment suggests that it may be a potential target for immunotherapy.
Milestones and Key performance Indicators to Assess the Capacity of Rapid Response Teams in the Eastern Mediterranean Region: A Methodological Approach
Authors: Elnosserry Sherein , Buliva Evans , Elkholy Amgad Abdalla , Abubakar Abdinasir , Koya Shaffi Fazaludeen , mahboob Amira 
Published: August 2023

Abstract
Background: A country’s capabilities to conduct proper response activities to outbreaks depend on its capacity for early detection and operational efficiency for a timely response through its frontline healthcare workers including public health rapid response teams (RRTs). RRT capacity and operation vary among the countries in the Eastern Mediterranean region (EMR) have never been systematically assessed, therefore, WHO EMRO decided to define a set of indicators that could be used for the evaluation of RRTs' capacity and performance among its member countries. The purpose of the present study was to develop performance measures for assessment of RRT in EMR.
Methods: Extensive literature review was carried out for critical elements/themes/categories of RRTs evaluation. Data were extracted using the “descriptive-analytical” method. An initial list of 126 indicators covering 8 themes/categories/ dimensions emerged. Using a modified Delphi paradigm, a multidisciplinary panel group of experts in outbreak response, epidemiology, public health, microbiology, social mobilization, and public health management assisted in the development and selection of the final set of indicators. A final list of 15 performance indicators was developed guided by the theory of change, logic model for RRT, and the RRT cycle.
Results: The final list covered 8 categories including workforce of RRTs, presence of standard operating procedures for RRT operations, financial and logistic support of RRTs deployment, timeliness of RRT operations/ activities, documentation and reporting of RRT response, interventional response of RRTs and monitoring and evaluation of RRT activities. Experts agreed upon 15 KPIs in 8 domains related to RRT operations and activities.
Conclusion: The developed KPIs are suggested to be used to guide policies and decisions related to RRT performance, operations, and evaluation.
Shift in prevalence and systemic inflammation levels from NAFLD to MAFLD: a population-based cross-sectional study
Authors: Liu Qingdan , Han Meilan , Li Meilan , Huang Xiaoyin , Feng Ruimei , Li Wanxin , Chen Jun , He Haiying , Zheng Wenxin , Hu Zhijian , Du Shanshan , Ye Weimin 
Published: August 2023

Abstract
Background
The difference in prevalence and systemic inflammatory (SI) status from non-alcoholic fatty liver disease (NAFLD) to newly defined metabolic dysfunction-associated fatty liver disease (MAFLD) was less reported. Therefore, we estimated the prevalence and summarized the systemic inflammation levels of MAFLD and NAFLD in a general population from Southern China to explore the potential effect of the transformation of disease definition.
Methods
In total, 6718 subjects aged 35–74 years old from a general population cohort were enrolled. Logistic regression and restricted cubic spline (RCS) models were used to examine the relationship between 15 SI indicators and NAFLD and MAFLD. The receiver operating characteristic (ROC) curve was used to investigate their predictive value for MAFLD and NAFLD.
Results
The prevalence of MAFLD and NAFLD was 34.7% and 32.4%, respectively. Their overlapping rate was 89.7%, while only 8.3% and 1.9% of participants were MAFLD-only and NAFLD-only. Among three FLD groups, the MAFLD-only group had the highest levels of 8 SI indicators. The non-FLD group had the lower levels of all 15 SI indicators compared with all FLD subgroups. The odds ratios (ORs) of 10 SI indicators were significant in both multivariable-adjusted logistic regression and RCS analyses of MAFLD or NAFLD. ROC analysis showed that the AUC values of all SI were lower than 0.7 in both MAFLD and NAFLD.
Conclusions
MAFLD could cover more FLD than NAFLD, and the MAFLD-only group had a more severe inflammation status, whereas the NAFLD-only exhibited lower levels.