bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation of Mouse Neutrophils
Authors: Wishart Andrew L. , Swamydas Muthulekha , Lionakis Michail S. 
Published: September 2023

AbstractNeutrophils represent the first line of defense against bacterial and fungal pathogens. Indeed, patients with inherited or acquired qualitative and quantitative neutrophil defects are at high risk for developing bacterial and fungal infections and suffering adverse outcomes from these infections. Therefore, research aiming at defining the molecular factors that modulate neutrophil effector function under homeostatic conditions and during infection is essential for devising strategies to augment neutrophil function and improve the outcomes of infected individuals. This article describes reproducible density‐gradient‐centrifugation‐based as well as positive and negative immunomagnetic selection protocols that can be applied in any laboratory to harvest large numbers of highly enriched and highly viable neutrophils from the bone marrow of mice. In another protocol, we also present a method that combines gentle enzymatic tissue digestion with a positive immunomagnetic selection technique or fluorescence‐activated cell sorting (FACS) to harvest highly pure and highly viable preparations of neutrophils directly from mouse tissues such as the kidney, the liver, or the spleen. Mouse neutrophils isolated by these protocols can be used to examine several aspects of cellular function ex vivo, including pathogen binding, phagocytosis, and killing, neutrophil chemotaxis, oxidative burst, degranulation, and cytokine production, and for performing neutrophil adoptive transfer experiments. © 2023 Wiley Periodicals LLC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.Basic Protocol 1: Isolation of Neutrophils from Mouse Bone Marrow Using Positive Immunomagnetic SeparationAlternate Protocol 1: Purification of Neutrophils from Bone Marrow Using Negative Immunomagnetic SeparationAlternate Protocol 2: Purification of Neutrophils from Bone Marrow Using Histopaque‐Based Density Gradient CentrifugationBasic Protocol 2: Isolation of Neutrophils from Mouse Tissues Using Positive Immunomagnetic SeparationAlternate Protocol 3: Isolation of Neutrophils from Mouse Tissues Using FACS
Research on the impact of environment, social and governance performance on corporate valuation: Evidence from China
Authors: NI Xinbo , Yuan Fangying , Gao Huiwen 
Published: September 2023

AbstractWith the greater popularity and recognition of the environment, social, and governance concept, it has increasingly become the focus of investors and enterprises in corporate valuation, capital allocation, strategic planning, and investment decision‐making. The environment, social, and governance framework covers many factors that affect a company's long‐term financial performance and sustainable development, so the environment, social, and governance characteristics of the company will reflect the long‐term value it can create in the future. Previous studies have rarely confirmed a strong positive correlation between corporate environment, social and governance performance, and valuation, and even if confirmed, they have not explained how environment, social, and governance performance affect valuation. Therefore, the originality of this paper lies in explaining how environment, social, and governance affect valuation, as well as discovering which factors in environment, social, and governance affect valuation, and which characteristics of the company will strengthen or weaken this impact. This paper uses a two‐way fixed effect model to study the impact of environment, social, and governance performance on the valuation of listed companies in Shanghai and Shenzhen A‐share markets from 2014 to 2021. Research findings: Enterprise environment, social, and governance performance can significantly improve its valuation, and the impact of environmental and social performance is more significant. Mediating effect test found that enterprise environment, social, and governance performance improves enterprise valuation by reducing the cost of capital, increasing investor attention, and reducing corporate financial risk. The moderating effect found that compared to state‐owned enterprises, the valuation of non‐state enterprises is highly influenced by environment, social, and governance performance, and corporate information transparency can also promote the transformation of environment, social, and governance performance to valuation. After a series of robustness and endogeneity tests, the results can still support the conclusions of this article. The contribution of this study is to provide empirical evidence for the positive economic consequences of environment, social, and governance performance, which has certain implications for enterprises and investors to attach importance to environment, social, and governance performance, and for government departments to improve environment, social and governance incentive policies. Due to the limitations of selecting only A‐share listed companies for the sample, further research is needed on the applicability of the conclusions of this article to other countries or small and medium‐sized enterprises. In future research, we need to continue to investigate whether there are any other influencing mechanisms that have not been discovered by us.
Covalent Organic Framework‐based Solid‐State Electrolytes, Electrode Materials, and Separators for Lithium‐ion Batteries
Authors: Zhu Youlong , Bai Qiaoshuang , Ouyang Shan , Jin Yinghua , Zhang Wei 
Published: September 2023

AbstractThe increasing global energy consumption has led to the rapid development of renewable energy storage technologies. Lithium‐ion batteries (LIBs) have been extensively studied and utilized for reliable, efficient, and sustainable energy storage. Nevertheless, designing new materials for LIB applications with high capacity and long‐term stability is highly desired but remains a challenging task. Recently, covalent organic frameworks (COFs) have emerged as superior candidates for LIB applications due to their high porosity, well‐defined pores, highly customizable structure, and tunable functionalities. These merits enable the preparation of tailored COFs with predesigned redox‐active moieties and suitable porous channels that can improve the lithium‐ion storage and transportation. This review summarizes the recent progress in the development of COFs and their composites for a variety of LIB applications, including (quasi) solid‐state electrolytes, electrode materials, and separators. Finally, the challenges and potential future directions of employing COFs for LIBs are also briefly discussed, further promoting the foundation of this class of exciting materials for future advances in energy‐related applications.
Using species distribution modeling to generate relative abundance information in socio‐politically unstable territories: Conservation of Felidae in the central‐western region of Mexico
Authors: Charre‐Medellín Juan F. , Ferrer‐Ferrando David , Monterrubio‐Rico Tiberio C. , Fernández‐López Javier , Acevedo Pelayo 
Published: September 2023

AbstractThe distribution range and population abundance of species provide fundamental information on the species–habitat relationship required for management and conservation. Abundance inherently provides more information about the ecology of species than do occurrence data. However, information on abundance is scarce for most species, mainly at large spatial scales. The objective of this work was, therefore, to provide information regarding the population status of six wild felids inhabiting territories in Mexico that are inaccessible or politically unstable. This was done using species distribution models derived from occurrence data. We used distribution data at a continental scale for the wild felids inhabiting Mexico: jaguar (Panthera onca), bobcat (Lynx rufus), ocelot (Leopardus pardalis), cougar (Puma concolor), margay (Leopardus wiedii), and jaguarundi (Herpailurus yagouaroundi) to predict environmental suitability (estimated by both Maxent and the distance to niche centroid, DNC). Suitability was then examined by relating to a capture rate‐based index, in a well‐monitored area in central western Mexico in order to assess their performance as proxies of relative abundance. Our results indicate that the environmental suitability patterns predicted by both algorithms were comparable. However, the strength of the relationship between the suitability and relative abundance of local populations differed across species and between algorithms, with the bobcat and DNC, respectively, having the best fit, although the relationship was not consistent in all the models. This paper presents the potential of implementing species distribution models in order to predict the relative abundance of wild felids in Mexico and offers guidance for the proper interpretation of the relationship between suitability and population abundance. The results obtained provide a robust information base on which to outline specific conservation actions and on which to examine the potential status of endangered species inhabiting remote or politically unstable territories in which on‐field monitoring programs are not feasible.
An inconvenient trend: Decadal decline in ground height of swamps in Southern Illinois
Authors: Middleton Beth A. 
Published: September 2023

AbstractAn understanding of the long‐term trends of ground height and sedimentation in a landscape context can provide a framework to better understand the impacts of agricultural development on floodplain processes. This study examined long‐term changes in ground height using surface elevation tables (SETs) and sediment deposition measurements in the Cache River floodplains of Southern Illinois including Taxodium distichum swamps in Crawford Tract and Eagle Pond from 2019 to 2022 and in Deer Pond and Snake Hole from 2005 to 2022. The mean ground heights of these swamps decreased by a mean of −0.33, −033, −0.28 and −0.19 cm year−1, respectively. Annual sediment deposition on feldspar markers in Crawford Tract was negligible from 2020 to 2022 (range of annual deposition depth: <0.1 to 0.4 cm). However, sediment deposition at Deer Pond and Snake Hole was higher from 2006 to 2021 (range of annual deposition depth: <0.1–2.5 and <1 to 2.0 cm, respectively). In some years, the sites appeared scoured, and the feldspar markers had disappeared. The loss of ground height in these swamps could be related to erosion, increased precipitation and/or downcutting of the channel following the construction of a major diversion to dewater the floodplain via the Post Creek Cutoff in the early 1900s. Despite the limitations of this study toward revealing specific mechanisms linking floodplain erosion to channel downcutting and the engineered features of this landscape, this study clearly showed that Cache floodplains have lost height in past decades with potential consequences for the conservation of natural forests.
Trans‐N‐alkylation Covalent Exchanges on 1,3,4‐Trisubstituted 1,2,3‐Triazolium Iodides
Authors: Akacha Rania , Abdelhedi‐Miladi Imen , Montarnal Damien , Ben Romdhane Hatem , Drockenmuller Eric 
Published: September 2023

Abstract1,3,4‐Trisubstituted 1,2,3‐triazolium salts having either aliphatic or benzylic substituents at the N‐1 and N‐3 positions were synthesized in two steps involving: i) copper(I) catalyzed azide‐alkyne 1,3‐dipolar cycloaddition (CuAAC), and ii) N‐alkylation of the 1,2,3‐triazole intermediates. Trans‐N‐alkylation reactions in bulk and in the presence of excess methyl iodide were monitored by 1H NMR spectroscopy for each 1,2,3‐triazolium molecular model. By assigning the different formed species and their respective evolution with time, it was possible to conclude that trans‐N‐alkylation exchange reactions are significantly faster for benzylic substituents than for aliphatic ones. Furthermore, the exchange reactions are noticeably faster at the N‐3 position than at the N‐1 position most likely due to the steric hindrance induced by the neighboring C‐4 substituent. The kinetics of trans‐N‐alkylation reactions are thus influenced by both the chemical nature of the N‐1 and N‐3 substituents and the regiochemistry of the 1,2,3‐triazolium group. This provides important structural design rules to improve the properties of thermosetting covalent adaptable networks involving trans‐N‐alkylation of 1,2,3‐triazolium salts.
The development of laterite weathering profiles as a function of rainfall and time: A geophysical approach
Authors: Barton Benjamin C. , Nelson Stephen T. , McBride John H. , Bickmore Barry R. , Spring Isaiah , Wells Leeza , Van Katwyk Peter , Wolfe Eugene E. 
Published: September 2023

AbstractLaterite weathering profile (LWP) thicknesses are functions of precipitation rate and time, but their exact dependence on them is uncertain. We investigate LWP development on ground surfaces in Hawai'i that are neither aggrading nor eroding across substrates from 0.01 to 4 Ma and rainfall rates of <250 to >3000 mm/a. The Hawaiian Islands provide an excellent opportunity for LWP studies across climates and over millions of years on a single rock type, basalt. LWP weathering rates are usually determined by geochemical approaches. We present a geophysical method once bedrock ages and precipitation rates are known. We employed multichannel analysis of surface waves and horizontal‐to‐vertical spectral ratio methods. Results indicate that >70% of the variability in LWP thickness is due to precipitation and bedrock age. The remainder is attributed to measurement uncertainty and heterogeneity in the permeability of basalt. LWPs develop by two paths. Dry (<1000 mm/a) areas have a negative water balance with evapotranspiration exceeding rainfall. LWPs thicken until they reach a steady state where the storage capacity of the saprolite precludes the percolation of water into subjacent lava. In wetter areas, downslope interflow produces thick laterite wedges near coastlines that migrate upslope over ~1 Ma. Subsequently, they thicken and reach a steady state where precipitation cannot deliver water through the vadose zone. LWPs' thickness (T) increases as a function of time (t) and precipitation (P) according to the expression (95% confidence):
. The first partial derivative, or weathering rate, is
. Weathering rates decrease with time and are strong functions of t and P. These expressions add considerable insight into the rates and processes driving weathering at large scales and can also be solved for t, giving a rough estimate of the time of landscape formation of eroded surfaces.
Detection of decadal time‐series changes in flow hydrology in eastern Australia: Considerations for river recovery and flood management
Authors: Arash Amir Mohammad , Fryirs Kirstie , Ralph Timothy J. 
Published: September 2023

AbstractSince European colonisation, many coastal rivers of New South Wales (NSW) have been highly modified by channelisation, flow regulation, sediment mining, intensive grazing and agriculture, deforestation, and riparian vegetation and wood removal. Since the late 1980s, nearly 55% of these rivers have undergone a significant ‘re‐greening’ and geomorphic recovery with changes to instream and riparian roughness. However, little research has been undertaken to quantify if there have been any coeval changes in flow hydrology. Approximately 7000 flow hydrographs with one‐hour time‐steps from 117 gauges on 45 study rivers (17 of 20 coastal catchments of NSW) were used to assess changes to in‐channel and overbank hydrology over decadal timeframes. Hydrograph shape and attenuation characteristics for three morphologically defined flow stages were analysed: in‐channel fresh, high flow and overbank flood. Time‐series analysis was used to quantify changes in flow hydrology from the early‐20th century to present and identify trends occurring between known degradation phases (1910s–1940s and 1940s–1980s) and a recovery phase (1990s to present) for these rivers. Our findings indicate that, on average, and for some key examples, changes in flow hydrology are occurring. Flows are slowing and attenuating as indicated by decreases in flood wave celerity and hydrograph kurtosis, skewness and rate of rise and increases in peak‐to‐peak travel time, flood peak attenuation and flood wave attenuation index. The most significant changes have occurred since the 1980s with the most noticeable effects on the behaviour of high flows (around the bankfull stage). However, there is not a consistent direction change in these indicators in all places. We use these findings to categorise the flow mitigation signal of the study regions and catchments and discuss the implication for river and flood management.
Lactarius badiosanguineus: A new record to Indian mycobiota from Jammu and Kashmir, India
Authors: Verma Komal , Mehmood Tahir , Sharma Yash Pal 
Published: September 2023

AbstractThe genus Lactarius is cosmopolitan in distribution and forms an ectomycorrhizal association with varied host plants. In the present study, samples of Lactarius badiosanguineus were collected during a fungal survey from different regions of Jammu province, Jammu and Kashmir, India. Identification was carried out through combined morpho‐anatomical and nuclear ribosomal DNA internal transcribed spacer (ITS) sequence analysis that confirmed its position as Lactarius badiosanguineus. This taxon is reported here for the first record from India. Detailed morphological descriptions, color photographs, line drawings, and molecular phylogeny are provided.
Assessing the multitargeted antidiabetic potential of three pomegranate peel‐specific metabolites: An in silico and pharmacokinetics study
Authors: Gull Hina , Ikram Aqsa , Khalil Anees Ahmed , Ahmed Zahoor , Nemat Arash 
Published: September 2023

AbstractDiabetes is a chronic metabolic disorder that occurs due to impaired secretion of insulin, insulin resistance, or both. Recent studies show that the antidiabetic drugs used to control hyperglycemic levels are associated with undesirable adverse effects. Therefore, developing a safe and effective medicine with antidiabetic potential is needed. In this context, in silico studies are considered a rapid, effectual, and cost‐effective method in drug discovery procedures. It is evident from the literature that plant‐based natural components have shown promising outcomes in drug development to alleviate various diseases and hence have diversified the screening of potential antidiabetic agents. Purposely, in the present study, an in silico approach was performed on three Punica granatum peel metabolites (punicalin, punicalagin, and ellagic acid). All these three compounds were docked against nine protein targets involved in glucose metabolism (GFAT, PTP1β, PPAR‐ᵞ, TKIR, RBP4, α‐amylase, α‐glucosidase, GCK, and AQP‐2). These three pomegranate‐specific compounds demonstrated significant interactions with GFAT, PTP1β, PPAR‐ᵞ, TKIR, RBP4, α‐amylase, α‐glucosidase, GCK, and AQP‐2 protein targets. Specifically, punicalin, punicalagin, and ellagic acid revealed significant binding scores (−9.2, −9.3, −8.1, −9.1, −8.5, −11.3, −9.2, −9.5, −10.1 kcal/mol; −10, −9.9, −8.5, −8.9, −10.4, −9.0, −10.2, −9.4, −9.0 kcal/mol; and −8.1, −8.0, −8.0, −6.8, −8.7, −7.8, −8.3, −8.1, −8.1 kcal/mol, respectively), with nine protein targets mentioned above. Hence, punicalin, punicalagin, and ellagic acid can be promising candidates in drug discovery to manage diabetes. Furthermore, in vivo and clinical trials must be conducted to validate the outcomes of the current study.
DICER1‐mutated rhabdomyosarcoma of the ovary with teratoid features
Authors: Lethongsavarn Vincent , Vieille Pierre , Kikweta Makhama Jeanos , Azmani Rihab , Lafrance Webert , Khneisser Pierre , Truffaut Nathalene , Alame Melissa , Genestie Catherine , Gaspar Nathalie , Diedhiou Abdoulaye , Croce Sabrina , Le Loarer François 
Published: September 2023

AbstractDICER1‐mutated rhabdomyosarcoma is a rare, emerging entity with a predilection for the gynecologic and genitourinary tracts. We report here a case of DICER1‐mutated rhabdomyosarcoma of the ovary in a 14 years old girl which displayed interspersed mature teratoid glands, neuroectodermal rosettes and immature blastematous‐like tubes. Morphologically the sarcomatous component predominated, corresponding to a high grade spindle cell rhabdomyosarcoma with botryoid features. Islets of cartilage were present. The sarcomatous proliferation encased the teratoid glands, forming cambium layer‐like arrangements. The sarcoma cells were Myogenin and MYOD1 positive, the neuroectodermal rosettes expressed SALL4 along with cytokeratins and EMA and were negative for Inhibin; immature blastematous‐like tubes were negative for SALL4 and Inhibin. Whole RNA‐ and targeted DNA‐sequencing revealed two DICER1 mutations in exon 26: c.5113G>A: p.(Glu1705Lys) and exon 12: c.1642C>T: p.(Gln548X). The sarcomatous component harbored a complex genetic profile while the teratoid component was diploid, none of the above displayed abnormality of 12p. DICER1‐mutated sarcomas display pathological features similar to embryonal rhabdomyosarcomas, botryoid type. They also display heterogeneous features combining cartilage foci, teratoid mature glands, immature blastematous‐like tubes and/or neuroectodermal components. Molecular testing remains necessary to confirm the diagnosis. Further studies need to clarify the nosology of DICER1‐mutated sarcomas and devise specific therapeutic strategies.
Land Use and Land Cover Change Detection Using the Random Forest Approach: The Case of The Upper Blue Nile River Basin, Ethiopia
Authors: Tikuye Birhan Getachew , Rusnak Milos , Manjunatha Busnur R. , Jose Jithin 
Published: September 2023

AbstractMonitoring land use change dynamics is critical for tackling food security, climate change, and biodiversity loss on a global scale. This study is designed to classify land use and land cover in the upper Blue Nile River Basin (BNRB) using a random forest (RF) algorithm. The Landsat images for Landsat 45, Landsat 7, and Landsat 8 are used for classification purposes. The study area is classified into seven land use/land cover classes: cultivated lands, bare lands, built‐ups, forests, grazing lands, shrublands, and waterbodies. The accuracy of classified images is 83%, 85%, and 91% using the Kappa index of agreements. From 1983 to 2022 periods, cultivated lands and built‐up areas increased by 47541 and 1777 km2, respectively, at the expense of grazing lands, shrublands, and forests. Furthermore, the area of water bodies has increased by 662 km2 due to the construction of small and large‐scale irrigation and hydroelectric power generation dams. The main factors that determine agricultural land expansion are related to population growth. Therefore, land use and land cover change detection using a random forest is an important technique for multispectral satellite data classification to understand the optimal use of natural resources, conservation practices, and decision‐making for sustainable development.