bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary intervention in gestational diabetes: a qualitative study of the acceptability and feasibility of a novel whole-diet intervention in healthcare professionals
Authors: Kusinski Laura Caroline , Richards Rebecca , Jones Danielle L. , Turner Elizabeth , Hughes Deborah J , Dyson Pamela , Ahern Amy L. , Meek Claire Louise 
Published: August 2023

AbstractGestational diabetes is treated with medical nutrition therapy, delivered by healthcare professionals; however, the optimal diet for affected women is unknown. Randomised controlled trials, such as the DiGest (Dietary Intervention in Gestational Diabetes) trial, will address this knowledge gap, but the acceptability of whole-diet interventions in pregnancy is unclear. Whole-diet approaches reduce bias but require high levels of participant commitment and long intervention periods to generate meaningful clinical outcomes. We aimed to assess healthcare professionals’ views on the acceptability of the DiGest dietbox intervention for women with gestational diabetes and to identify any barriers to adherence which could be addressed to support good recruitment and retention to the DiGest trial. Female healthcare professionals (n 16) were randomly allocated to receive a DiGest dietbox containing 1200 or 2000 kcal/d including at least one weeks’ food. A semi-structured interview was conducted to explore participants’ experience of the intervention. Interviews were audio-recorded, transcribed verbatim and analysed thematically using NVivo software. Based on the findings of qualitative interviews, modifications were made to the dietboxes. Participants found the dietboxes convenient and enjoyed the variety and taste of the meals. Factors which facilitated adherence included participants having a good understanding of study aims and sufficient organisational skills to facilitate weekly meal planning in advance. Barriers to adherence included peer pressure during social occasions and feelings of deprivation or hunger (affecting both standard and reduced calorie groups). Healthcare professionals considered random allocation to a whole-diet replacement intervention to be acceptable and feasible in a clinical environment and offered benefits to participants including convenience.
Model-free adaptive robust control based on TDE for robot with disturbance and input saturation
Authors: Liu Xia , Wang Lu , Yang Yong 
Published: August 2023

AbstractA model-free adaptive robust control based on time delay estimation (TDE) is proposed for robot in the presence of disturbance and input saturation. TDE is utilized to estimate the complicated nonlinear terms of the robot including unknown dynamics and disturbance, and a TDE error observer is developed to estimate the inevitable TDE error. When the input torque of the robot exceeds the upper or lower limit of the input saturation, an auxiliary system and a saturation deviation boundary adaptive law are employed to mitigate the negative impact of input saturation on the position tracking. Finally, the robust control law is obtained by backstepping. The stability of the closed-loop system is proved by Lyapunov functions, and the validity of the proposed method is demonstrated by comparative simulations and experiments. Compared with the model-based controllers and other model-free controllers, the proposed method does not necessitate the accurate dynamic model of the complicated system and with lower computation. Moreover, it can guarantee the desired position tracking performance of the robot even subject to disturbance and input saturation simultaneously.
Factors influencing tropical lizard reproduction vary by microhabitat but not forest type
Authors: Swartwout Meredith C. , Willson J. D. 
Published: August 2023

Abstract
To understand mechanisms behind enigmatic declines of tropical reptiles, knowledge of species interactions and how they vary over space and time is important. Some tropical lizard population dynamics can be highly influenced by egg survival. Yet relatively few studies have examined relationships between lizard reproductive success and egg predators across forest and microhabitat types. In this study, we examined variation in probability of egg depredation, predatory ant abundance, prey availability, and the number of lizards and eggs encountered across four different forest types (abandoned agroforestry, abandoned plantation, secondary forest, and old-growth forest) and three microhabitats (buttress, fallen log, and leaf-litter) at La Selva Biological Station, Costa Rica. Based on previous studies, we made three hypotheses about how lizard egg abundance, egg survival, and predatory ant numbers would be related across microhabitat and forest type. Of these hypotheses, only one was supported: we found more lizard eggs in buttress and fallen log microhabitats than leaf-litter. We did not observe any differences in lizard reproduction or numbers of invertebrates by forest type alone. Based on patterns observed in this study, we suggest that future studies investigating tropical leaf-litter lizard declines focus on environmental variation at the microhabitat scale.
Efficacy of meaning-centered group psychotherapy in Chinese patients with cancer: A randomized controlled trial
Authors: Wang Shuman , Zhu Yu , Wang Ziying , Zheng Mimi , Li Xiaoru , Zhang Yu , Wan Hongwei 
Published: August 2023

AbstractObjectivesMeaninglessness is one of the most common psychological problems in cancer patients, which can lead to anxiety, depression and psychological distress, and diminished quality of life. Recent evidence indicates that meaning-centered group psychotherapy (MCGP) effectively enhances the meaning in life among cancer patients. This study aimed to evaluate the impact of MCGP on the meaning in life, post-traumatic growth, psychological distress, and fear of recurrence among Chinese cancer patients with a favorable prognosis.MethodsSixty-six cancer patients were randomly assigned to either the MCGP group (n = 33) or the control group (n = 33). Participants in the MCGP group underwent a 4-week, 8-session MCGP, while those in the control group received usual care. Meaning in life, post-traumatic growth, psychological distress, and fear of recurrence were assessed at both baseline and postintervention to evaluate the impact of the intervention. The intervention outcomes were analyzed using paired t-tests or analysis of covariance, as appropriate.ResultsPatients in the MCGP group demonstrated significant improvements in meaning in life, post-traumatic growth, and fear of recurrence from baseline to postintervention. In comparison to the control group, the MCGP group displayed positive effects on meaning in life and post-traumatic growth following the intervention. However, no significant effects were observed in terms of psychological distress and fear of recurrence.Significance of resultsOur research offers evidence supporting the effectiveness of MCGP in enhancing meaning in life and post-traumatic growth among Chinese cancer patients with a favorable prognosis.
Moving ‘red lines’: The Russian–Ukrainian war and the pragmatic (mis-)use of international law
Authors: dos Reis Filipe , Grzybowski Janis 
Published: August 2023

AbstractThe Russian invasion of Ukraine has widely been seen as a failure of the international legal order, which could neither stop Russia from launching a war of aggression, nor prevent the perpetration of international crimes. In such a reading, great power politics have (once again) trumped international law. We argue instead that international law plays a crucial part in the conflict by providing a semantic infrastructure, which the opposing parties use to justify their actions, try to re-draw limits of permissible action and negotiate changing ‘red lines’ with the enemy. Drawing on the notion of lawfare, we show how the pragmatic (mis-)use of international law flexibly delineates boundaries and stabilizes expectations between adversaries even as they are contested in the current war. We focus on claims about self-determination and self-defence to justify the use of force; categorizations of combatants; and weapons transfers and the status of third states. That international law can be violated or reinterpreted to breaking point does not make it irrelevant. To the contrary, it recalls its important role as a language of conflict and compromise, beyond strictly legalist as well as dismissive realist views.
Decadal Trends in the Oceanic Storage of Anthropogenic Carbon From 1994 to 2014
Authors: Müller Jens Daniel , Gruber N. , Carter B. , Feely R. , Ishii M. , Lange N. , Lauvset S. K. , Murata A. , Olsen A. , Pérez F. F. , Sabine C. , Tanhua T. , Wanninkhof R. , Zhu D. 
Published: August 2023

AbstractThe oceanic uptake and resulting storage of the anthropogenic CO2 (Cant) that humans have emitted into the atmosphere moderates climate change. Yet our knowledge about how this uptake and storage has progressed in time remained limited. Here, we determine decadal trends in the storage of Cant by applying the eMLR(C*) regression method to ocean interior observations collected repeatedly since the 1990s. We find that the global ocean storage of Cant grew from 1994 to 2004 by 29 ± 3 Pg C dec−1 and from 2004 to 2014 by 27 ± 3 Pg C dec−1 (±1σ). The storage change in the second decade is about 15 ± 11% lower than one would expect from the first decade and assuming proportional increase with atmospheric CO2. We attribute this reduction in sensitivity to a decrease of the ocean buffer capacity and changes in ocean circulation. In the Atlantic Ocean, the maximum storage rate shifted from the Northern to the Southern Hemisphere, plausibly caused by a weaker formation rate of North Atlantic Deep Waters and an intensified ventilation of mode and intermediate waters in the Southern Hemisphere. Our estimates of the Cant accumulation differ from cumulative net air‐sea flux estimates by several Pg C dec−1, suggesting a substantial and variable, but uncertain net loss of natural carbon from the ocean. Our findings indicate a considerable vulnerability of the ocean carbon sink to climate variability and change.
Effects of Precipitation Latent Heating on Structure and Evolution of Northeast China Cold Vortex: A PV Perspective
Authors: Fan Ziqi , Xue Ming , Zhu Kefeng , Luo Liping , Gao Zongting , Li Shangfeng 
Published: August 2023

AbstractBased on potential vorticity (PV) thinking, northeast China cold vortex (NCCV) corresponds to an upper‐level high PV anomaly from stratospheric PV downward intrusion. Within a vortex, latent heating from precipitation would produce a vertical dipole of PV anomalies that would affect structures and evolution of the vortex. In this paper, three‐dimensional structures and evolution of NCCV and, effects of latent heating from precipitation along bent‐back, cold and warm fronts on them are investigated based on convection‐allowing simulations for an intense NCCV case during 8–17 June 2012. Trajectory analysis shows that the negative upper‐level diabatic PV anomaly from bent‐back frontal precipitation, near the vortex center, is the dominant contributor to erosion of high PV in the vortex core region as it is advected in, leading to the weakening of the vortex. The negative PV anomalies along the cold and warm fronts, at the east‐to‐southeast side of the vortex, are mostly advected downstream away from the NCCV. In the middle troposphere, positive PV anomalies are primarily generated along fronts and the accumulated positive PV anomalies filling the vortex region help to reinforce the low‐level cyclonic circulation. The lower‐level PV is affected by surface heating and cooling through their effects on static stability, but such effects are periodic and create mainly diurnal variations. The NCCV eventually decays as the upper‐level vortex weakens due to significant PV erosion.
Advancing translational research for colorectal immuno-oncology
Authors: Thomas Elaine M. , Wright Josephine A. , Blake Stephen J. , Page Amanda J. , Worthley Daniel L. , Woods Susan L. 
Published: August 2023

AbstractColorectal cancer (CRC) is a common and deadly disease. Unfortunately, immune checkpoint inhibitors (ICIs) fail to elicit effective anti-tumour responses in the vast majority of CRC patients. Patients that are most likely to respond are those with DNA mismatch repair deficient (dMMR) and microsatellite instability (MSI) disease. However, reliable predictors of ICI response are lacking, even within the dMMR/MSI subtype. This, together with identification of novel mechanisms to increase response rates and prevent resistance, are ongoing and vitally important unmet needs. To address the current challenges with translation of early research findings into effective therapeutic strategies, this review summarises the present state of preclinical testing used to inform the development of immuno-regulatory treatment strategies for CRC. The shortfalls and advantages of commonly utilised mouse models of CRC, including chemically induced, transplant and transgenic approaches are highlighted. Appropriate use of existing models, incorporation of patient-derived data and development of cutting-edge models that recapitulate important features of human disease will be key to accelerating clinically relevant research in this area.
Body composition from birth to 2 years
Authors: Hills Andrew P. , Norris Shane A. , Byrne Nuala M. , Jayasinghe Sisitha , Murphy-Alford Alexia J. , Loechl Cornelia U. , Ismail Leila I. Cheikh , Kurpad Anura V. , Kuriyan Rebecca , Nyati Lukhanyo H. , Santos Ina S. , Costa Caroline S. , Wickramasinghe V. Pujitha , Lucas M. Nishani , Slater Christine , Yameen Ayesha , Ariff Shabina ,   Multi-center Infant Body Composition Reference Study (MIBCRS)
Published: August 2023

AbstractProviding all infants with the best start to life is a universal but challenging goal for the global community. Historically, the size and shape of infants, quantified by anthropometry and commencing with birthweight, has been the common yardstick for physical growth and development. Anthropometry has long been considered a proxy for nutritional status during infancy when, under ideal circumstances, changes in size and shape are most rapid. Developed from data collected in the Multicentre Growth Reference Study (MGRS), WHO Child Growth Standards for healthy infants and children have been widely accepted and progressively adopted. In contrast, and somewhat surprisingly, much less is understood about the ‘quality’ of growth as reflected by body composition during infancy. Recent advances in body composition assessment, including the more widespread use of air displacement plethysmography (ADP) across the first months of life, have contributed to a progressive increase in our knowledge and understanding of growth and development. Along with stable isotope approaches, most commonly the deuterium dilution (DD) technique, the criterion measure of total body water (TBW), our ability to quantify lean and fat tissue using a two-compartment model, has been greatly enhanced. However, until now, global reference charts for the body composition of healthy infants have been lacking. This paper details some of the historical challenges associated with the assessment of body composition across the first two years of life, and references the logical next steps in growth assessments, including reference charts.
Effector-dependent structural transformation of a crystalline framework with allosteric effects on molecular recognition ability
Authors: Hayashi Ryunosuke , Tashiro Shohei , Asakura Masahiro , Mitsui Shinya , Shionoya Mitsuhiko 
Published: August 2023

AbstractStructurally flexible porous crystals that combine high regularity and stimuli responsiveness have received attracted attention in connection with natural allostery found in regulatory systems of activity and function in biological systems. Porous crystals with molecular recognition sites in the inner pores are particularly promising for achieving elaborate functional control, where the local binding of effectors triggers their distortion to propagate throughout the structure. Here we report that the structure of a porous molecular crystal can be allosterically controlled by local adsorption of effectors within low-symmetry nanochannels with multiple molecular recognition sites. The exchange of effectors at the allosteric site triggers diverse conversion of the framework structure in an effector-dependent manner. In conjunction with the structural conversion, it is also possible to switch the molecular affinity at different recognition sites. These results may provide a guideline for the development of supramolecular materials with flexible and highly-ordered three-dimensional structures for biological applications.
HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells
Authors: Santos Mark F. , Rappa Germana , Karbanová Jana , Diana Patrizia , Cirrincione Girolamo , Carbone Daniela , Manna David , Aalam Feryal , Wang David , Vanier Cheryl , Corbeil Denis , Lorico Aurelio 
Published: August 2023

AbstractThe mechanism of human immunodeficiency virus 1 (HIV-1) nuclear entry, required for productive infection, is not fully understood. Here, we report that in HeLa cells and activated CD4+ T cells infected with HIV-1 pseudotyped with VSV-G and native Env protein, respectively, Rab7+ late endosomes containing endocytosed HIV-1 promote the formation of nuclear envelope invaginations (NEIs) by a molecular mechanism involving the VOR complex, composed of the outer nuclear membrane protein VAP-A, hyperphosphorylated ORP3 and Rab7. Silencing VAP-A or ORP3 and drug-mediated impairment of Rab7 binding to ORP3-VAP-A inhibited the nuclear transfer of the HIV-1 components and productive infection. In HIV-1-resistant quiescent CD4+ T cells, ORP3 was not hyperphosphorylated and neither VOR complex nor NEIs were formed. This new cellular pathway and its molecular players are potential therapeutic targets, perhaps shared by other viruses that require nuclear entry to complete their life cycle.
High resolution cryo-EM and crystallographic snapshots of the actinobacterial two-in-one 2-oxoglutarate dehydrogenase
Authors: Yang Lu , Wagner Tristan , Mechaly Ariel , Boyko Alexandra , Bruch Eduardo M. , Megrian Daniela , Gubellini Francesca , Alzari Pedro M. , Bellinzoni Marco 
Published: August 2023

AbstractActinobacteria possess unique ways to regulate the oxoglutarate metabolic node. Contrary to most organisms in which three enzymes compose the 2-oxoglutarate dehydrogenase complex (ODH), actinobacteria rely on a two-in-one protein (OdhA) in which both the oxidative decarboxylation and succinyl transferase steps are carried out by the same polypeptide. Here we describe high-resolution cryo-EM and crystallographic snapshots of representative enzymes from Mycobacterium smegmatis and Corynebacterium glutamicum, showing that OdhA is an 800-kDa homohexamer that assembles into a three-blade propeller shape. The obligate trimeric and dimeric states of the acyltransferase and dehydrogenase domains, respectively, are critical for maintaining the overall assembly, where both domains interact via subtle readjustments of their interfaces. Complexes obtained with substrate analogues, reaction products and allosteric regulators illustrate how these domains operate. Furthermore, we provide additional insights into the phosphorylation-dependent regulation of this enzymatic machinery by the signalling protein OdhI.
Evolutionary genomics of camouflage innovation in the orchid mantis
Authors: Huang Guangping , Song Lingyun , Du Xin , Huang Xin , Wei Fuwen 
Published: August 2023

AbstractThe orchid mantises achieve camouflage with morphological modifications in body color and pattern, providing an interesting model for understanding phenotypic innovation. However, a reference genome is lacking for the order Mantodea. To unveil the mechanisms of plant-mimicking body coloration and patterns, we performed de novo assembly of two chromosome-level genomes of the orchid mantis and its close relative, the dead leaf mantis. Comparative genomic analysis revealed that the Scarlet gene plays an important role in the synthesis of xanthommatin, an important pigment for mantis camouflage coloration. Combining developmental transcriptomic analysis and genetic engineering experiments, we found that the cuticle was an essential component of the ‘petal-like’ enlargement, and specific expression in the ventral femur was controlled by Wnt signaling. The prolonged expression of Ultrabithorax (Ubx) accompanied by femoral expansion suggested that Ubx determines leg remodeling in the early developmental stage. We also found evidence of evolution of the Trypsin gene family for insectivory adaptation and ecdysone-dependent sexual dimorphism in body size. Overall, our study presents new genome catalogs and reveals the genetic and evolutionary mechanisms underlying the unique camouflage of the praying mantis, providing evolutionary developmental insights into phenotypic innovation and adaptation.
In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system
Authors: Erdem Özgecan , Eş Ismail , Saylan Yeşeren , Atabay Maryam , Gungen Murat Alp , Ölmez Kadriye , Denizli Adil , Inci Fatih 
Published: August 2023

AbstractCurrent practices in synthesizing molecularly imprinted polymers face challenges—lengthy process, low-productivity, the need for expensive and sophisticated equipment, and they cannot be controlled in situ synthesis. Herein, we present a micro-reactor for in situ and continuously synthesizing trillions of molecularly imprinted polymeric nanoparticles that contain molecular fingerprints of bovine serum albumin in a short period of time (5-30 min). Initially, we performed COMSOL simulation to analyze mixing efficiency with altering flow rates, and experimentally validated the platform for synthesizing nanoparticles with sizes ranging from 52-106 nm. Molecular interactions between monomers and protein were also examined by molecular docking and dynamics simulations. Afterwards, we benchmarked the micro-reactor parameters through dispersity and concentration of molecularly imprinted polymers using principal component analysis. Sensing assets of molecularly imprinted polymers were examined on a metamaterial sensor, resulting in 81% of precision with high selectivity (4.5 times), and three cycles of consecutive use. Overall, our micro-reactor stood out for its high productivity (48-288 times improvement in assay-time and 2 times improvement in reagent volume), enabling to produce 1.4-1.5 times more MIPs at one-single step, and continuous production compared to conventional strategy.
River export of macro- and microplastics to seas by sources worldwide
Authors: Strokal Maryna , Vriend Paul , Bak Mirjam P. , Kroeze Carolien , van Wijnen Jikke , van Emmerik Tim 
Published: August 2023

AbstractSeas are polluted with macro- (>5 mm) and microplastics (<5 mm). However, few studies account for both types when modeling water quality, thus limiting our understanding of the origin (e.g., basins) and sources of plastics. In this work, we model riverine macro- and microplastic exports to seas to identify their main sources in over ten thousand basins. We estimate that rivers export approximately 0.5 million tons of plastics per year worldwide. Microplastics are dominant in almost 40% of the basins in Europe, North America and Oceania, because of sewage effluents. Approximately 80% of the global population live in river basins where macroplastics are dominant because of mismanaged solid waste. These basins include many African and Asian rivers. In 10% of the basins, macro- and microplastics in seas (as mass) are equally important because of high sewage effluents and mismanaged solid waste production. Our results could be useful to prioritize reduction policies for plastics.