bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sorghum bicolorSbHSP110 has an elongated shape and is able of protecting against aggregation and replacing human HSPH1/HSP110 in refolding and disaggregation assays
Authors: Franco Juliana C. , Nogueira Maria L. C. , Gandelini Gabriela M. , Pinheiro Glaucia M. S. , Gonçalves Conrado C. , Barbosa Leandro R. S. , Young Jason C. , Ramos Carlos H. I. 
Published: February 2023

AbstractPerturbations in the native structure, often caused by stressing cellular conditions, not only impair protein function but also lead to the formation of aggregates, which can accumulate in the cell leading to harmful effects. Some organisms, such as plants, express the molecular chaperone HSP100 (homologous to HSP104 from yeast), which has the remarkable capacity to disaggregate and reactivate proteins. Recently, studies with animal cells, which lack a canonical HSP100, have identified the involvement of a distinct system composed of HSP70/HSP40 that needs the assistance of HSP110 to efficiently perform protein breakdown. As sessile plants experience stressful conditions more severe than those experienced by animals, we asked whether a plant HSP110 could also play a role in collaborating with HSP70/HSP40 in a system that increases the efficiency of disaggregation. Thus, the gene for a putative HSP110 from the cereal Sorghum bicolor was cloned and the protein, named SbHSP110, purified. For comparison purposes, human HsHSP110 (HSPH1/HSP105) was also purified and investigated in parallel. First, a combination of spectroscopic and hydrodynamic techniques was used for the characterization of the conformation and stability of recombinant SbHSP110, which was produced folded. Second, small‐angle X‐ray scattering and combined predictors of protein structure indicated that SbHSP110 and HsHSP110 have similar conformations. Then, the chaperone activities, which included protection against aggregation, refolding, and reactivation, were investigated, showing that SbHSP110 and HsHSP110 have similar functional activities. Altogether, the results add to the structure/function relationship study of HSP110s and support the hypothesis that plants have multiple strategies to act upon the reactivation of protein aggregates.
How to deal with customer and supplier concentration to attain sustainable financial growth? The role of business strategy
Authors: Ahsan Tanveer , Mirza Sultan Sikandar , Gull Ammar Ali , Majeed Muhammad Ansar 
Published: February 2023

AbstractFor effective supply chain management, firms should focus on the relationship with their immediate stakeholders in the supply chain, namely, suppliers (upstream) and customers (downstream). This study investigates the impact of customer and supplier concentration as well as business strategy on sustainable financial growth, using financial data of 2021 Chinese non‐financial firms listed from 2006 to 2020. Additionally, it explores the moderating effect of business strategy on the relationship between customer and supplier concentration and sustainable financial growth. We find that higher customer and supplier concentration weakens the bargaining power of Chinese firms, which reduces their financial growth. However, an appropriate business strategy can help such firms achieve sustainable financial growth. We find that an aggressive business strategy moderates the negative impact of supplier concentration, while a defensive business strategy moderates the negative impact of customer concentration on financial growth. Finally, our results for the direct impact of customer and supplier concentration as well as business strategy hold for state‐owned and non‐state‐owned firms. However, we find differences regarding the moderating impact of business strategy between state‐owned and non‐state‐owned firms. Our results are robust to time and industry fixed effects, alternate proxies of financial growth and regression techniques.
Use of scanning electron microscopy and energy dispersive X‐ray spectroscopy to identify key fouling species during alternating tangential filtration
Authors: Sundar Vikram , Zhang Da , Qian Xianghong , Wickramasinghe S. Ranil , Smelko John Paul , Carbrello Christina , Jabbour Al Maalouf Yara , Zydney Andrew L. 
Published: February 2023

AbstractAlternating tangential flow filtration (ATF) has become one of the primary methods for cell retention and clarification in perfusion bioreactors. However, membrane fouling can cause product sieving losses that limit the performance of these systems. This study used scanning electron microscopy and energy dispersive X‐ray spectroscopy to identify the nature and location of foulants on 0.2 μm polyethersulfone hollow fiber membranes after use in industrial Chinese hamster ovary cell perfusion bioreactors for monoclonal antibody production. Membrane fouling was dominated by proteinaceous material, primarily host cell proteins along with some monoclonal antibody. Fouling occurred primarily on the lumen surface with much less protein trapped within the depth of the fiber. Protein deposition was also most pronounced near the inlet/exit of the hollow fibers, which are the regions with the greatest flux (and transmembrane pressure) during the cyclical operation of the ATF. These results provide important insights into the underlying phenomena governing the fouling behavior of ATF systems for continuous bioprocessing.
Mycosis fungoides with Psoriasiform plaques: A case report and review of the literature
Authors: Beyzaee Amir Mohammad , Jahantigh Neda , Goldust Mohammad , Rahmatpour Rokni Ghasem , Babaei Mahsa , Ghoreishi Bahare , Sadathosseini Sepideh 
Published: February 2023

AbstractMycosis fungoides (MF) is the most common variant of primary skin T‐cell lymphoma. It typically manifests as an indolent progressing cutaneous eruption with erythematous scaly patches or plaques. Due to the nonspecific pathological findings, it can be easily misdiagnosed as psoriasis. A 34‐year‐old woman with a history of psoriasiform plaques for 12 years was referred to our dermatology clinic. In the beginning, the diagnosis of psoriasis was made and topical steroids were prescribed: it did not exhibit any clinical improvement. During the visit, skin biopsy was performed and the diagnosis of MF was confirmed. Treatment with PUVA, prednisolon, methotrexate, topical ointment including ucerin, urea, and clobetasol were initiated. Significant improvement in all lesions were observed after 1 month of the treatment, and within a year, the disease improved dramatically after PUVA therapy. In refractory cases of psoriasiform plaques that are progressive and/or ulcerative despite the optimal treatment, biopsy is required and a possible diagnosis of MF should be kept in mind.
Selective Electroreduction of Oxalic Acid to Glycolic Acid by Mesoporous TiO2 Spheres
Authors: Xu Wenjing , Cheng Yingying , Hou Jing , Kang Peng 
Published: February 2023

AbstractReducing oxalic acid to glycolic acid electrochemically remains a significant challenge due to the high overpotential and low selectivity and stability. This study uses mesoporous TiO2 spheres of anatase phase as catalysts to reduce oxalic acid to glycolic acid with low overpotential. The maximum Faradaic efficiency of glycolic acid reaches 73.9 % at −0.5 V vs RHE. The mesoporous structure not only increases the specific surface area, but also limits the escape of intermediate glyoxalic acid from the catalyst surface due to the space‐limiting effect of the mesoporous channel, such that oxalic acid can be deeply reduced to glycolic acid. Moreover, a mechanism for oxalic acid reduction is proposed, and the Ti3+/Ti4+ redox pair plays an important role in the reduction of oxalic acid.
Nickel‐Catalyzed Dry Reforming of Methane via Modulating the Zirconia Shapes
Authors: Tan Ruifan , Wang Ping , Guo Luyao , Chen Zupeng , Lin Ronghe , Mou Xiaoling , Ding Yunjie 
Published: February 2023

AbstractThe shape of the carriers has a profound impact on the catalytic performance of metal‐supported catalysts. Herein, ZrO2 of different shapes (nanorods‐like, spherical or irregular nanoparticles, demoted as RZ, SPZ, and IPZ, respectively) are adopted to disperse Ni species to assess the shape effects on the catalytic performance in dry reforming of methane. To this end, the fresh and spent catalysts are thoroughly characterized by multiple techniques including N2 sorption, powder X‐ray diffraction, temperature‐programmed reduction with H2, thermogravimetric analyses, transmission electron microscopy, Raman and X‐ray photoelectron spectroscopies. It is revealed that the reducibility of NiO species follows the orders of Ni/RZ<Ni/SPZ<Ni/IPZ. Consequently, Ni/RZ and Ni/SPZ show lower dehydrogenation activity than Ni/IPZ, but the more abundant lattice oxygen species promote the gasification of carbonaceous deposits. Therefore, the shape design of the carriers provides a new perspective in mediating the catalytic performance of Ni/ZrO2 in the reforming reaction.
Electrochemical Characterization of Charge Storage at Anodes for Sodium‐Ion Batteries Based on Corncob Waste‐Derived Hard Carbon and Binder
Authors: Bottoni Luca , Darjazi Hamideh , Sbrascini Leonardo , Staffolani Antunes , Gabrielli Serena , Pastore Genny , Tombesi Alessia , Nobili Francesco 
Published: February 2023

AbstractSodium‐ion batteries (SIBs) represent a potential alternative to lithium‐ion batteries in large‐scale energy storage applications. To improve the sustainability of SIBs, the utilization of anode carbonaceous materials produced from biomass and the selection of a bio‐based binder allowing an aqueous electrode processing are fundamental. Herein, corncobs are used as raw material for the preparation of hard carbon and it is also used as cellulose sources for the synthesis of carboxymethyl cellulose (CMC) binder. The corncob‐derived electrodes deliver a high discharge capacity of around 264 mAhg−1 at 1 C (300 mAg−1), with promising capacity retention (84 % after 100 cycles) and good rate capability. Additionally, this work expands the fundamental insight of the sodium storage behavior of Hard Carbons through an electrochemical approach, suggesting that the reaction mechanism is controlled by capacitive process in the sloping voltage region, while the diffusion‐controlled intercalation is the predominant process in the low‐voltage plateau.
Static Binding and Dynamic Transporting‐Based Design of Specific Ring‐Chain‐Ring Acetylcholinesterase Inhibitor: From Galantamine to Natural Product
Authors: Zhang Zhiyang , Lv Jianwu , Wang Yu , Yu Hongli , Guo Baolin , Zhai Jihang , Wang Chaojie , Zhao Yuan , Fan Fangfang , Luo Wen 
Published: February 2023

AbstractAcetylcholinesterase (AChE) is a key target for the current symptomatic treatment of Alzheimer's disease, and galantamine is a clinical anticholinesterase drug with transiently acting characteristic and good selectivity for AChE. The present theoretical‐experimental work improves the drug‘s residence time without reducing the inhibition effect, thus providing a crucial breakthrough for modifying the inhibitor of AChE with better kinetic behavior. The static binding and dynamic delivery properties acquired from atomic view reveal that the galantamine simply occupies a catalytic anionic site, and its release from AChE needs only ∼8.6 kcal/mol. Both of these may cause the short residence time of galantamine. The hotspots and most favorable transport mechanism are identified, and the hydrogen bond and aromatic stacking interactions are observed to play crucial roles for galantamine binding and release in AChE. The typical peripheral anionic site arisen at the delivery process would provide another key occupation to enhance the anti‐release ability for inhibitors. The compound with “specific‐ring‐chain‐ring” framework with detailed beneficial modification scheme is summarized, which may improve the residence time of the inhibitor in AChE. The thermodynamic and dynamic properties of galantamine derivatives are also studied. Based on dictamnine, a natural alkaloid, two novel eligible derivatives are designed, synthesized and evaluated, which verifies our prediction. Multiple computational approaches and experimental combinations probably provide a train of thought from both static and dynamic views to modify or design appropriate inhibitors on the basis of specific binding and transportation features.
Flexible Organic Framework‐Modified Membranes for Osmotic Energy Harvesting
Authors: Lin Tao , Zhang Lei , Li Chao , Fu Yong‐Hong , Gao Longcheng , Hou Jun‐Li 
Published: February 2023

Comprehensive SummaryBioinspired membranes are advantageous in capturing the osmotic energy. However, the conventional hybrid membranes possess low harvesting power density due to their low interfacial ionic transport efficiency and high internal resistance. Herein, a new kind of hybrid membranes consisting of porous polymer and flexible organic frameworks was developed. The 3D porous framework enables ions to flux with high efficiency at the polymer‐framework interface, resulting in high osmotic energy harvesting efficiency. By systematically screening the pore size of the frameworks, the output power density as high as 5.7 W/m2 was achieved under 50‐fold KCl salinity gradient.
Top advances of the year: Neuro‐oncology
Authors: Barden Mary M. , Omuro Antonio M. 
Published: February 2023

AbstractManagement of brain tumors has been challenging given the limited therapeutic options and disabling morbidities associated with central nervous system (CNS) dysfunction. This review focuses on recent developments in the field, with an emphasis on clinical management. The growing clinical trials landscape reflects advanced insights into cancer immunology and genomics and the need to address molecular and clinical heterogeneity. Recent phase 3 trials investigating anti–PD‐1 immunotherapies, particularly nivolumab, have failed to demonstrate improved survival in glioblastoma, underscoring the need to better understand the complexity of CNS immunologic surveillance. Conversely, targeted therapies have accounted for several US Food and Drug Administration approvals extended to brain tumors, particularly therapies directed to BRAF V600E mutations and TRAK fusions, underscoring a need to routinely screen patients for these rare molecular abnormalities. In primary CNS lymphoma, attention has turned to long‐term outcomes of consolidation therapies, and recent studies have highlighted the excellent disease control afforded by high‐dose chemotherapy and stem cell transplantation. Meningiomas remain a focus of investigations, with preliminary promising results observed with octreotide combined with mTOR inhibition, and immunotherapy with single‐agent pembrolizumab. Finally, proton radiotherapy has emerged as a novel alternative for leptomeningeal metastases from solid tumors, which can now be treated more safely with craniospinal irradiation and monitored by the enumeration of circulating tumor cells in the cerebrospinal fluid as a biomarker. Taken together, these incremental advances have improved outcomes in select brain tumor patient populations, whereas ongoing clinical trials hold the promise of meaningful advances and breakthroughs for larger proportions of patients with brain tumors.
Hybrid optimization enabled multi‐aggregator‐based charge scheduling of electric vehicle in internet of electric vehicles
Authors: Suresh Pandian , Shobana Selvaraj , Ramya Ganesan , Belsam Jeba Ananth Manasea Selvin 
Published: February 2023

SummaryIn modern days, electric vehicles are quickly industrialized as well as their penetration is also increased highly, which brings more challenges for the power system. The electric vehicle charge scheduling process is vital to encourage the daily usage of the electric vehicle. However, irregular charging methods for electric vehicles may disturb voltage security areas because of their stochastic characteristics. Moreover, an electric vehicle requires recurrent charging owing to its constrained battery capacity, but it is a time‐consuming process. In this article, an effective charge scheduling model is devised using the fractional social sea lion optimization (Fr‐SSLO) algorithm. At first, IoEV network is simulated along with charge station and electric vehicle location. Furthermore, multi aggregator‐based charge scheduling is done for increasing the profit and amount of scheduled electric vehicles. Then, routing is performed based on developed Fr‐SSLO algorithm. Moreover, several fitness measures, including distance, energy and variable energy purchase are included. Here, the devised Fr‐SSLO model is designed by integrating fractional calculus (FC) and sea lion optimization (SLnO) technique along with SOA. After the completion of routing process, charge scheduling is performed based on developed Fr‐SSLO approach. Moreover, various fitness functions are also considered for computing better performance.
Quality characteristics of non‐gluten biscuit developed from optimized cream and orange‐fleshed sweetpotato flour
Authors: Oloniyo Rebecca Olajumoke , Omoba Olufunmilayo Sade , Awolu Olugbenga Olufemi 
Published: February 2023

AbstractOrange‐fleshed sweetpotato (OFSP; Ipomoea batatas L.) is a new variety of sweetpotato widely accepted as a staple food in African countries due to its nutritional and health benefits. The aim of this study was to determine the quality characteristics of value added biscuit developed from optimized non‐gluten blends. King J orange‐fleshed sweetpotato (UMUSPO1), Mother's Delight orange‐fleshed sweetpotato (UMUSPO2) and cream‐fleshed sweetpotato were used. Sweetpotato storage roots were processed into flour and were incorporated with soy concentrate, date palm and sweetpotato starch. The blends were optimized using an experimental design of response surface methodology; the optimized blends were used to produce biscuits, and the biscuits were evaluated for total phenol, total flavonoid, ferric reducing antioxidant power (FRAP) and effective concentration (EC50), which is a concentration of the sample required to obtain a 50% antioxidant effect. The physical qualities and consumer acceptability of the developed and control biscuits were also assessed. The results obtained showed that the total phenolic values ranged from 2.32 to 12.54 mg GAE/g, total flavonoid values ranged from 1.11 to 12.20 mg QE/g, and FRAP values ranged from 4.38 to 22.54 mg AEE/g; EC50 of some antioxidant properties was observed to be less than (<) 2; the weight of the biscuit ranged between 4.50 and 5.59 g, diameter of the biscuit ranged between 5.30 and 6.70 cm, and the spread ratio ranged from 8.83 to 12.89. The sensory properties of the biscuit varied significantly (p < 0.05) with one another in terms of the aroma, appearance, crunchiness, taste and overall acceptability. This research showed that the optimized OFSP flour (OFSPK2) produced a good quality biscuit with the highest total phenolic, total flavonoid, and FRAP, and it exhibited good physical qualities. OFSPK2 biscuit had the highest score for its overall acceptability and compared favourably with the 100% wheat flour biscuit (control sample).
Automated quantification of measurable residual disease in chronic lymphocytic leukemia using an artificial intelligence‐assisted workflow
Authors: Bazinet Alexandre , Wang Alan , Li Xinmei , Jia Fuli , Mo Huan , Wang Wei , Wang Sa A. 
Published: February 2023

AbstractDetection of measurable residual disease (MRD) in chronic lymphocytic leukemia (CLL) is an important prognostic marker. The most common CLL MRD method in current use is multiparameter flow cytometry, but availability is limited by the need for expert manual analysis. Automated analysis has the potential to expand access to CLL MRD testing. We evaluated the performance of an artificial intelligence (AI)‐assisted multiparameter flow cytometry (MFC) workflow for CLL MRD. We randomly selected 113 CLL MRD FCS files and divided them into training and validation sets. The training set (n = 41) was gated by expert manual analysis and used to train the AI model. We then compared the validation set (n = 72) MRD results obtained by the AI‐assisted analysis versus those by expert manual analysis using the Pearson correlation coefficient and Bland–Altman plot method. In the validation set, the AI‐assisted analysis correctly categorized cases as MRD‐negative versus MRD‐positive in 96% of cases. When comparing the AI‐assisted analysis versus the expert manual analysis, the Pearson r was 0.8650, mean bias was 0.2237 log10 units, and the 95% limit of agreement (LOA) was ±1.0282 log10 units. The AI‐assisted analysis performed sub‐optimally in atypical immunophenotype CLL and in cases lacking residual normal B cells. When excluding these outlier cases, the mean bias improved to 0.0680 log10 units and the 95% LOA to ±0.2926 log10 units. An automated AI‐assisted workflow allows for the quantification of MRD in CLL with typical immunophenotype. Further work is required to improve performance in atypical immunophenotype CLL.
Realizing the untapped promise of single‐session interventions for eating disorders
Authors: Schleider Jessica L. , Smith Arielle C. , Ahuvia Isaac 
Published: February 2023

AbstractObjectiveMultilevel treatment barriers prevent up to 80% of individuals experiencing eating disorders (EDs) from accessing care. This treatment gap creates a critical need to identify interventions that are accessible, easily completable, and optimized for effectiveness by targeting core mechanisms linked to ED onset and maintenance. We propose single‐session interventions (SSIs) as a promising path toward catalyzing innovation in the development of accessible, effective ED interventions. SSIs are structured programs that intentionally involve one encounter with a program or provider; they may serve as stand‐alone or adjunctive clinical supports. All SSIs are built to acknowledge that any session might be someone's last—and that any single session can nonetheless yield meaningful clinical benefit.MethodWe define SSIs, summarize research supporting their utility for ED symptoms and other mental health problems, and recommend future directions for work in this domain.ResultsSingle‐session interventions may hold promise to reduce some ED symptoms and risk factors, including restrictive eating and negative body image. Steps toward realizing this promise include (1) testing whether existing evidence‐based SSIs (e.g., for depression) can also reduce EDs, risk factors, and symptoms; (2) developing novel SSIs that target modifiable ED risk factors and symptoms largely unaddressed by SSIs, such as purging and binge eating; (3) studying diverse implementation pathways; (4) capitalizing on SSIs' transdiagnostic utility to broaden funding opportunities; and (5) educating ED researchers and clinicians about SSIs.DiscussionUnderstanding the strengths and limits of mechanism‐targeted SSIs for ED‐related problems could be a low‐risk, high‐reward avenue toward reducing EDs at scale.Public SignificanceMost individuals experiencing EDs never access any form of treatment, creating an urgent need to identify ED interventions built to overcome barriers to engaging with care. This Forum article introduces SSIs as a promising path to rapidly developing and testing accessible, evidence‐based ED supports; supplementing existing ED treatment models; and reducing the individual, familial, and societal burdens of EDs at scale.