bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple amino acid supplementations to reduce dietary protein for pigs during early and late finisher periods under commercial conditions
Authors: Song Wenxin , Wu Zijuan , Li Wenli , Li Yali 
Published: January 2023

AbstractBackgroundWith the easy availability and competitive prices of crystalline amino acids (AAs), the reduction of dietary crude protein (CP) for pigs during early and late finisher periods is possible under commercial conditions. Two experiments were conducted to assess the growth efficiency of early and late‐finishing pigs fed with protein‐restricted diets supplemented with Lys, Met, Thr, Trp, Val, Ile and His. In Experiment 1, 840 early finishing pigs were allocated to four dietary treatments with CP levels designed at 150, 142, 134, and 126 g kg−1 diet. In Experiment 2, 768 late‐finishing pigs were allotted to four dietary treatments providing CP levels at 140, 130, 120, and 110 g kg−1 diet.ResultsIn Experiment 1, the data showed that CP levels could be decreased from 150 to 126 g kg−1 without adversely affecting performance of early finishing pigs as no significant difference was observed for final bodyweight, average daily gain (ADG), feed to gain ratio (F:G), or average daily feed intake (ADFI). In Experiment 2, late‐finishing pigs consuming 120 g kg−1 CP tended to have the highest ADG and lowest F:G whereas those fed the 110 g kg−1 CP diet showed the opposite trend. Based on quadratic analysis, the optimum CP levels to maximize ADG and minimize F:G were 126 and 127 g kg−1, respectively.ConclusionThese findings showed that dietary CP levels could be decreased to 126 g kg−1 for early finishing pigs while improved performance was noted in late‐finishing pigs consuming 120 g kg−1 CP. © 2023 Society of Chemical Industry.
The archaeal KEOPS complex possesses a functional Gon7 homolog and has an essential function independent of the cellular t6A modification level
Authors: Wu Pengju , Gan Qi , Zhang Xuemei , Yang Yunfeng , Xiao Yuanxi , She Qunxin , Ni Jinfeng , Huang Qihong , Shen Yulong 
Published: January 2023

AbstractKinase, putative Endopeptidase, and Other Proteins of Small size (KEOPS) is a multisubunit protein complex conserved in eukaryotes and archaea. It is composed of Pcc1, Kae1, Bud32, Cgi121, and Gon7 in eukaryotes and is primarily involved in N6‐threonylcarbamoyl adenosine (t6A) modification of transfer RNAs (tRNAs). Recently, it was reported that KEOPS participates in homologous recombination (HR) repair in yeast. To characterize the KEOPS in archaea (aKEOPS), we conducted genetic and biochemical analyses of its encoding genes in the hyperthermophilic archaeonSaccharolobus islandicus. We show that aKEOPS also possesses five subunits, Pcc1, Kae1, Bud32, Cgi121, and Pcc1‐like (or Gon7‐like), just like eukaryotic KEOPS. Pcc1‐like has physical interactions with Kae1 and Pcc1 and can mediate the monomerization of the dimeric subcomplex (Kae1‐Pcc1‐Pcc1‐Kae1), suggesting that Pcc1‐like is a functional homolog of the eukaryotic Gon7 subunit. Strikingly, none of the genes encoding aKEOPS subunits, including Pcc1 and Pcc1‐like, can be deleted in the wild type and in a t6A modification complementary strain named TsaKI, implying that the aKEOPS complex is essential for an additional cellular process in this archaeon. Knock‐down of the Cgi121 subunit leads to severe growth retardance in the wild type that is partially rescued in TsaKI. These results suggest that aKEOPS plays an essential role independent of the cellular t6A modification level. In addition, archaeal Cgi121 possesses dsDNA‐binding activity that relies on its tRNA 3ʹ CCA tail binding module. Our study clarifies the subunit organization of archaeal KEOPS and suggests an origin of eukaryotic Gon7. The study also reveals a possible link between the function in t6A modification and the additional function, presumably HR.
Fragmented care and chronic illness patient outcomes: A systematic review
Authors: Joo Jee Young 
Published: January 2023

AbstractAimThis systematic review examined recent studies on fragmented care of patients with chronic illnesses in the United States to examine the association between fragmented care and patient outcomes.DesignSystematic review.MethodsStudies published from January 1, 2012, to June 1, 2022, were selected from four electronic databases (PubMed, CINAHL, PsycINFO, and Web of Science), following the Cochrane protocols and PRISMA statements. Based on inclusion and exclusion criteria, ten studies that examined associations published between 2015 and 2021 were selected. A methodological assessment was conducted with the Quality Assessment Tool for Observational Cohort and Cross‐Sectional Studies. The studies selected for this systematic review were rated as having fair methodological rigor. The protocol of this review was registered in the International Prospective Register of Systematic Reviews (PROSPERO registration number: CRD42021285379). Because of the heterogeneity of the selected studies' data, a systematic narrative synthesis of the extracted data was conducted.ResultsThree common measures for fragmented care and outcomes were synthesized. A synthesis of the studies found significant association between fragmented care and adverse outcomes of chronic illnesses (emergency department visits, utilization of diagnostic tests, and healthcare costs). Despite the heterogeneity of significant findings between fragmented care and patient outcomes, the relationship between these outcomes and fragmented care was significant. This systematic review provides clear evidence of the association between care fragmentation and its adverse effects on individuals with chronic illnesses. However, mixed relationship findings were also reported.ConclusionGiven the demands of overcoming fragmented care in healthcare settings in the United States, nurse managers, healthcare leaders, and policymakers should utilize this evidence to reduce fragmented care strategies. It is recommended that nurse researchers and other healthcare practitioners conduct further studies to understand the contexts and mechanisms of fragmented care and develop theoretical frameworks for care fragmentation and chronic illness outcomes.
Control of lipid metabolism by the dynamic and nutrient‐dependent post‐translational modification O‐GlcNAcylation
Authors: Schulz Céline , Lemaire Quentin , Berthier Alexandre , Descat Amandine , Kouach Mostafa , Vercoutter‐Edouart Anne‐Sophie , Yazidi‐Belkoura Ikram El , Hardivillé Stéphan , Goossens Jean‐François , Lefebvre Philippe , Lefebvre Tony 
Published: January 2023

AbstractO‐GlcNAcylation is a post‐translational modification belonging to the large group of glycosylations. It consists of the modification of cytoplasmic, nuclear, and mitochondrial proteins with a single N‐acetylglucosamine residue by O‐GlcNAc transferase (OGT). Despite its structural simplicity, O‐GlcNAcylation orchestrates many functions inside the cell. This modification regulates fatty acids synthesis, fat storage, and utilization. The generation of white and brown adipocyte‐OGT knock‐out mice has highlighted the marked interference of O‐GlcNAcylation in adiposity and, as a consequence, in metabolic pathologies. OGT is more especially involved in the regulation of lipolysis, and thermogenesis in brown adipose tissue. In addition, O‐GlcNAcylation directly regulates fatty acid synthase, the main enzyme responsible for fatty acids synthesis, and other lipogenic enzymes and transcription factors. Nevertheless, only a few studies reported connections between O‐GlcNAcylation and homeostasis of cholesterol or its derivatives. This knowledge gap is surprising due to the crucial importance of cholesterol in structuring animal biological membranes and as a precursor of a wide variety of biological compounds. Here, we review the current literature about this topic and discuss future prospects in the field.Key points
As a PTM, O‐GlcNAcylation exponentially expands protein functions.
O‐GlcNAcylation orchestrates many biological functions in living beings including metabolic fluxes.
O‐GlcNAcylation is crucial for fat storage and mobilization, and for fatty acid synthesis but its function in the metabolism of other lipid compounds is less documented.
Utilization of a strong promoter combined with the knockout of protease genes to improve the yield of Vip3Aa in Bacillus thuringiensisBMB171
Authors: Li Xuelian , Zhang Yanli , Zhan Yunda , Tian Hongwei , Yan Bing , Cai Jun 
Published: January 2023

AbstractBackgroundVip3Aa is an insecticidal protein secreted by some Bacillus thuringiensis strains during vegetative growth. It has excellent insecticidal activity, its mechanism of action is different from that of Cry protein, and it can delay the development of pest resistance. To date, Vip3Aa has been widely used in genetically modified Bt crops. However, the secretion of Vip3Aa by industrial production strains is usually very low. Moreover, most of the Vip3Aa in the medium is degraded by proteases, limiting its application as a biopesticide.ResultsWe report a novel constitutive strong promoter from B. thuringiensis, Prsi, which directs the abundant expression of vip3Aa in B. thuringiensis BMB171. Furthermore, to reduce the degradation of Vip3Aa caused by proteases, we constructed B. thuringiensis mutants in which different protease genes were knocked out. We found that the degradation of Vip3Aa was greatly inhibited and its yield was significantly improved in a mutant that lacked all three protease genes.ConclusionOur results provide a new strategy to enhance the production of Vip3Aa in B. thuringiensis and have reference value for the research and development of novel bioinsecticides. © 2023 Society of Chemical Industry.
Smart water management
Authors: Owen David Lloyd 
Published: January 2023

AbstractSmart water enables utilities, regulators, and customers to make more timely and informed decisions about how they use and regard their water resources. It has been developed to assist demand management by influencing customer behavior and reducing network leakage, lowering energy consumption, and avoiding deploying assets that are not actually needed. Smart water has seen an evolution toward monitoring wastewater applications. Challenges include the need for common operating standards and more cohesive national policy frameworks. As a result, smart water adoption occurs on a utility‐by‐utility basis.
Multi‐Layered PtAu Nanoframes and Their Light‐Enhanced Electrocatalytic Activity via Plasmonic Hot Spots
Authors: Lee Soohyun , Lee Jaewon , Lee Sungwoo , Haddadnezhad MohammadNavid , Oh Myeong Jin , Zhao Qiang , Yoo Sungjae , Liu Lichun , Jung Insub , Park Sungho 
Published: January 2023

AbstractHere, the rational design of complex PtAu double nanoframes (DNFs) for plasmon‐enhanced electrocatalytic activity toward the methanol oxidation reaction (MOR) is reported. The synthetic strategy for the DNFs consists of on‐demand multiple synthetic chemical toolkits, including well‐faceted Au growth, rim‐on selective Pt deposition, and selective Au etching steps. DNFs are synthesized by utilizing Au truncated octahedrons (TOh) as a starting template. The outer octahedral (Oh) nanoframes (NFs) nest the inner TOh NFs, eventually forming DNFs with a tunable intra‐nanogap distance. Residual Au adatoms on Pt skeletons act as light entrappers and produce plasmonic hot spots between inner and outer frames through localized surface plasmon resonance (LSPR) coupling, which promotes enhanced electrocatalytic activity for the MOR. Importantly, the correlation between the gap‐induced hot carriers and electrocatalytic activity is evaluated. The highest catalytic activity is achieved when the gap is the narrowest. To further harness their light‐trapping capability, hierarchically structured triple NFs (TNFs) are synthesized, wherein three NFs are entangled in a single entity with a high density of hot regions, exhibiting superior electrocatalytic activity toward the MOR with a sixfold larger current density under light irradiation compared to the dark conditions.
Writing Entrepreneurial-Theorizing Outcomes
Authors: Shepherd Dean A. , Patzelt Holger 
Published: January 2023

AbstractThere are several excellent “From the Editor” notes, but in this chapter, we offer something more hands-on with a perspective directly applicable to entrepreneurship. We believe that sometimes papers are rejected, not because the research is fundamentally flawed but because authors miss some of the fundamentals of a strong paper. Therefore, this chapter aims to offer some advice on writing entrepreneurship papers. We offer (1) 11 simple rules for constructing an entrepreneurship paper; (2) six templates for what is to be included in each section of an entrepreneurship paper and provide illustrations of how we have previously executed these tasks; and (3) five heuristics for improving one’s writing quality.
A Lean Approach to Entrepreneurial Theorizing
Authors: Shepherd Dean A. , Patzelt Holger 
Published: January 2023

AbstractWe offer lean scholarship as an approach to developing a portfolio of high-quality, high-impact papers. Indeed, our scholarly identity is typically generated and reinforced by our portfolio of published papers than by any one paper. By lean scholarship, we refer to iterative experimentation, stakeholder engagement, and collective learning in generating a portfolio of papers. Lean scholarship starts with an entrepreneurial mindset and involves creating a minimum viable paper, exploring its validity, and adding a plausible paper to one’s portfolio. Lean scholarship requires managing the portfolio by periodically deciding whether to persevere, pivot, or terminate each paper. Therefore, this chapter moves away from our emphasis on a single paper to think more broadly about the entrepreneurial process of constructing and managing a portfolio of entrepreneurship studies
Microscale investigation of phase transformation and plasticity in multi-crystalline shape memory alloy using discrete dislocation–transformation method
Authors: Sakhaei Amir Hosein , Shafiee Mahmood 
Published: January 2023

AbstractMartensitic phase transformation and plasticity are two primary mechanisms of deformation in shape memory alloys (SMAs) and the interaction between them influences the behaviour of SMA during cyclic loading, specifically the pseudoelasticity behaviour and the shape memory effect. This interaction, which occurs in microscale, affects the reversibility and eventually the actuation capacity of SMAs. In order to capture this interaction in microscale, a discrete dislocation–transformation model was developed in Sakhaei et al. (Mech Mater 97:1–18, 2016) and was applied to simulate the single-crystalline NiTi samples under thermo-mechanical loads. In this study, the microscale coupling between phase transformation and plasticity as well as grain size and orientation effects is investigated in multi-crystalline shape memory alloys under thermal and mechanical loading by using the discrete dislocation–transformation framework through the representative numerical simulations. The results illustrated the dependency of dislocation slip and martensitic transformation to crystalline orientations as well as grain size and grain boundary densities in the multi-crystalline SMAs.
The core for housing markets with limited externalities
Authors: Klaus Bettina , Meo Claudia 
Published: January 2023

AbstractWe propose a variant of the housing market model à la Shapley and Scarf (in J Math Econ 1:23–37, 1974) that incorporates a limited form of externality in consumption; that is, agents care both about their own consumption (demand preferences) and about the agent who receives their endowment (supply preferences). We consider different domains of preference relations by taking demand and supply aspects of preferences into account. First, for markets with three agents who have (additive) separable preferences such that all houses and agents are acceptable, the strong core is nonempty; a result that can be neither extended to the unacceptable case nor to markets with a larger number of agents. Second, for markets where all agents have demand lexicographic preferences (or all of them have supply lexicographic preferences), we show that the strong core is nonempty, independent of the number of agents and the acceptability of houses or agents, and possibly multi-valued.
Ultrasound‐Guided Vacuum‐Assisted Excision to Treat Intraductal Papilloma
Authors: He Ping , Lei Yu‐Tao , Chen Wen , Shen Wei‐Wei , Fu Peng , Zhao Hong‐Mei , Cui Li‐Gang 
Published: January 2023

AbstractBackgroundTo evaluate the value of ultrasound‐guided vacuum‐assisted excision (US‐guided VAE) in the treatment of intraductal papillomas, including intraductal papillomas with atypical ductal hyperplasia (ADH), and to evaluate the lesion characteristic features affecting the local recurrence rate.Materials and methodsBetween August 2011 and December 2020, 91 lesions of 91 patients underwent US‐guided VAE and were diagnosed with intraductal papilloma with or without ADH. The recurrence rate of intraductal papilloma was evaluated on follow‐up US. The lesion characteristic features were analyzed to identify the factors affecting the local recurrence rate.ResultsThe local recurrence rate of intraductal papillomas removed by US‐guided VAE was 7.7% (7/91), with the follow‐up duration 12–92 months (37.4 ± 23.9 months). Of the 91 patients, five cases diagnosed as intraductal papilloma with ADH did not recur, with the follow‐up time 12–47 months (26.4 ± 14.4 months). There were no malignant transformation in all 91 cases during the follow‐up period. All 7 patients recurred 7–58 months (22.8 ± 19.2 months) after US‐guided VAE. There were no significant differences between the non‐recurrence and recurrence groups in terms of age, side, distance from nipple, lesion size, BI‐RADS category, with ADH, or history of excision (p > 0.05).ConclusionsUS‐guided VAE is an effective method for the treatment of intraductal papilloma, including intraductal papilloma with ADH. It avoids invasive surgical excision, but regular follow‐up is recommended to prevent recurrence or new onset due to multifocality. Any suspicious lesions during the follow‐up should be actively treated.
Establishment of a risk stratification model based on the combination of post-treatment serum squamous cell carcinoma antigen levels and FIGO stage of cervical cancer for treatment and surveillance decision-making
Authors: Shi Liu , Liu Yuxin , Li Junyun , Kou Jia , Ouyang Yi , Chen Foping , Huang Xiaodan , Huo Lanqing , Huang Lin , Cao Xinping 
Published: January 2023

Abstract
Objective
To develop a risk stratification model based on the International Federation of Gynecology and Obstetrics (FIGO) staging combined with squamous cell carcinoma antigen (SCC-Ag) for the classification of patients with cervical squamous cell carcinoma (CSCC) into different risk groups.

Methods
We retrospectively reviewed the data of 664 women with stage IIA–IVB CSCC according to the 2018 FIGO staging system who received definitive radiotherapy from March 2013 to December 2017 at the department of radiation oncology of Sun Yat-sen University Cancer Center. Cutoff values for continuous variables were estimated using receiver operating characteristic curve analysis. Using recursive partitioning analysis (RPA) modeling, overall survival was predicted based on the prognostic factors determined via Cox regression analysis. The predictive performance of the RPA model was assessed using the consistency index (C-index). Intergroup survival differences were determined and compared using Kaplan–Meier analysis and the log-rank test.

Results
Multivariate Cox regression analysis identified post-treatment SCC-Ag (< 1.35 ng/mL and > 1.35 ng/mL; hazard ratio (HR), 4.000; 95% confidence interval (CI), 2.911–5.496; P < 0.0001) and FIGO stage (II, III, and IV; HR, 2.582, 95% CI, 1.947–3.426; P < 0.0001) as the independent outcome predictors for overall survival. The RPA model based on the above prognostic factors divided the patients into high-, intermediate-, and low-risk groups. Significant differences in overall survival were observed among the three groups (5-year overall survival: low vs. intermediate vs. high, 91.3% vs. 76.7% vs. 29.5%, P < 0.0001). The predictive performance of the RPA model (C-index, 0.732; 95% CI, 0.701–0.763) was prominently superior to that of post-treatment SCC-Ag (C-index, 0.668; 95% CI, 0.635–0.702; P < 0.0001) and FIGO stage (C-index, 0.663; 95% CI, 0.631–0.695; P < 0.0001).

Conclusions
The RPA model based on FIGO staging and post-treatment SCC-Ag can predict the overall survival of patients with CSCC, thereby providing a guide for the formulation of risk-adaptive treatment and individualized follow-up strategies.
Adverse childhood experiences are associated with vascular changes in adolescents that are risk factors for future cardiovascular disease
Authors: Kellum Cailin E. , Kemp Keri M. , Mrug Sylvie , Pollock Jennifer S. , Seifert Michael E. , Feig Daniel I. 
Published: January 2023

Abstract
Background
Adverse childhood experiences (ACEs), such as abuse, neglect, and household dysfunction, are associated with a higher risk of cardiovascular disease (CVD) and indicators of future CVD risk in adulthood, such as greater vascular stiffness. The impact of ACEs in adolescence is unclear, and understanding how ACEs relate to blood pressure (BP) and vascular function during early life is key for the development of prevention strategies to reduce CVD risk. We hypothesized that exposure to ACEs would be associated with changes in central hemodynamics such as increased vascular stiffness and higher BP during adolescence.

Methods
This pilot study enrolled 86 adolescents recruited from the Children’s of Alabama. A validated ACE questionnaire was employed, and ACEs were modeled both as a continuous variable and a categorical variable (ACE ≥ 1 vs. ACE = 0). The primary outcomes used are considered to be indicators of future cardio-renal disease risk: aortic augmentation index normalized to 75 bpm (Alx75, a surrogate for vascular stiffness), carotid-femoral PWV (m/s), and ambulatory BP patterns.

Results
Adolescents with ACE ≥ 1 had significantly higher Alx75 (ACE: 5.2% ± 2.2 compared to no ACE: − 1.4% ± 3.0; p = 0.043). PWV only reflected this trend when adjustments were made for the body mass index. Adolescents with ACEs showed no differences in ambulatory BP patterns during the 24-h, wake, or sleep periods compared to adolescents with no ACEs.

Conclusions
ACEs were associated with higher AIx75 in adolescence, which is a risk factor for future CVD. Adolescence could present an opportunity for early detections/interventions to mitigate adverse cardiovascular outcomes in adulthood.

Graphical abstract
Chemical vapor deposition of carbohydrate-based polymers: a proof of concept study
Authors: Materna Philipp , Illek David , Unger Katrin , Thonhofer Martin , Wrodnigg Tanja M. , Coclite Anna Maria 
Published: January 2023

AbstractThe aim of this work is to investigate if vinyl-modified carbohydrate compounds are suitable monomers for thin film polymerization via chemical vapor deposition in a proof-of-concept study. Synthetic carbohydrate-based polymers are explored as biodegradable, biocompatible, and biorenewable materials. A thin film of synthetic polymers bearing sugar residues can also offer a good surface for cell attachment, and thus might be applied in biomaterials and tissue engineering. The possibility of having such thin film deposited from the vapor phase would ease the implementation in complex device architectures. For a proof-of-concept study, sugar vinyl compound monomers are synthesized starting from methyl α-d-glucopyranoside and polymerized by initiated chemical vapor deposition (iCVD) leading to a thin polymer layer on a Si-substrate. Thus, a successful vapor polymerization of the sugar compounds could be demonstrated. Infrared spectroscopy shows that no unwanted crosslinking reactions take place during the vapor deposition. The solubility of the polymers in water was observed in situ by spectroscopic ellipsometry.
Graphical abstract