bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isorhynchophylline alleviates cartilage degeneration in osteoarthritis by activating autophagy of chondrocytes
Authors: Jiang Jieyun , Li Jin , Xiong Chenwei , Zhou Xindie , Liu Ting 
Published: March 2023

Abstract
Context
Osteoarthritis is a common degenerative disease, the cause of it is still unknown, and the treatment mainly focuses on improving symptoms. Studies have found that Isorhynchophylline (Isorhy) has antioxidant, anti-inflammatory, antiproliferative and neuroprotective effects.

Objective
This study investigates the role and mechanism of Isorhy in OA.

Methods
The destabilized medial meniscus model was used to mimic OA. Fifteen male Sprague Dawley rats were partitioned into three portions: Normal group, OA group (surgery; normal saline treatment) and OA + Isorhy group (surgery; 50 μM Isorhy treatment) were performed on the first day of every week from the 5th to the 8th week after surgery. After 4 weeks of drug treatment, the rats have been processed without debridement of the knee specimens and fixed using 4% paraformaldehyde for two days. The morphological analysis was performed by H&E, Safranin O-Fast green staining and micro-CT analysis. The specimens were researched employing Micro-CT. In the part of the aggregate methods that were evaluated by qRT-PCR and western blot of the following proteins LC3II/LC3I, Beclin-1, ATG5, ATG7, MMP3 andMMP13. Akt/PI3K signaling related proteins (p-AKT, AKT, p-PI3K, PI3K, p-mTOR, mTOR) were detected by Western blot. BECLIN1 and MMP3 were detected by Immunofluorescence assay.

Results
In this present research, it was proved that autophagy-related and cartilage matrix-related proteins in osteoarthritis could be regulated by Isorhynchophylline treatment. The transcriptome sequencing results suggested the regulation was closely associated with PI3K/AKT/mTOR pathway, thereby alleviating osteoarticular inflammation. In-depth study showed that Isorhy could also affect OA in rat OA models, that was indicated by H&E, Safranin O-Fast green staining, and also micro-CT analysis.

Conclusion
Our findings indicated that Isorhy could be regarded as a prospective candidate for OA treatment.
The burden of illness in Lennox–Gastaut syndrome: a systematic literature review
Authors: Strzelczyk Adam , Zuberi Sameer M. , Striano Pasquale , Rosenow Felix , Schubert-Bast Susanne 
Published: March 2023

Abstract
Background
Lennox–Gastaut syndrome (LGS) is a severe developmental and epileptic encephalopathy characterized by drug-resistant epilepsy with multiple seizure types starting in childhood, a typical slow spike-wave pattern on electroencephalogram, and cognitive dysfunction.

Methods
We performed a systematic literature review according to the PRISMA guidelines to identify, synthesize and appraise the burden of illness in LGS (including “probable” LGS). Studies were identified by searching MEDLINE, Embase and APA PsychInfo, Cochrane’s database of systematic reviews, and Epistemonikos. The outcomes were epidemiology (incidence, prevalence or mortality), direct and indirect costs, healthcare resource utilization, and patient and caregiver health-related quality of life (HRQoL).

Results
The search identified 22 publications evaluating the epidemiology (n = 10), direct costs and resource (n = 10) and/or HRQoL (n = 5). No studies reporting on indirect costs were identified. With no specific ICD code for LGS in many regions, several studies had to rely upon indirect methods to identify their patient populations (e.g., algorithms to search insurance claims databases to identify “probable” LGS). There was heterogeneity between studies in how LGS was defined, the size of the populations, ages of the patients and length of the follow-up period. The prevalence varied from 4.2 to 60.8 per 100,000 people across studies for probable LGS and 2.9–28 per 100,000 for a confirmed/narrow definition of LGS. LGS was associated with high mortality rates compared to the general population and epilepsy population. Healthcare resource utilization and direct costs were substantial across all studies. Mean annual direct costs per person varied from $24,048 to $80,545 across studies, and home-based care and inpatient care were significant cost drivers. Studies showed that the HRQoL of patients and caregivers was adversely affected, although only a few studies were identified. In addition, studies suggested that seizure events were associated with higher costs and worse HRQoL. The risk of bias was low or moderate in most studies.

Conclusions
LGS is associated with a significant burden of illness featuring resistant seizures associated with higher costs and worse HRQoL. More research is needed, especially in evaluating indirect costs and caregiver burden, where there is a notable lack of studies.
Evaluation of a smartphone application for self-help for patients with social anxiety disorder: a randomized controlled study—SMASH
Authors: Schittenhelm Jan Marius , von Borell Christoph , Clément Celina , Schüller Johanna , Stangier Ulrich , Hoyer Juergen 
Published: March 2023

Abstract
Background
There is growing evidence that Internet-based cognitive behavioral therapy (ICBT) is as effective as a stand-alone treatment and helps facilitating access to treatment. Given the complexity of the treatment, we argue that the effect of ICBT could be even greater if guided by a therapist, as this could increase treatment adherence. We modified an established and well-evaluated treatment approach and developed a mobile application for treating social anxiety disorder (SAD). In the present study, we compare the efficacy of app use alone (APP) with video-based, therapist-guided app use (TG-APP) and with a wait-list control group (WLC) in terms of symptom reduction, and various secondary outcomes such as increase in quality of life or decrease of general psychological distress.

Methods/design
A within-between interaction design with randomization to one of three conditions will be used. In the APP condition, patients receive only the app without any additional contact with therapists, while in the TG-APP condition, therapists provide 8 sessions of video-based treatment in addition to using the app. The study will be conducted in two university outpatient treatment centers with reliably diagnosed SAD patients. The primary outcome will be defined as change in SAD symptoms, as measured by the Liebowitz Social Anxiety Scale (expert rating). Furthermore, a wide range of self-reports and clinician ratings for other symptoms (depression, general psychopathology) or quality of life will be used. A simulation-based power analysis for a 3 × 2 interaction effect (group × time) on the primary outcome in a linear mixed model resulted in a total sample size of N = 165.

Discussion
The present study will be one of the first to examine the additional benefit of therapist-guided video sessions regarding the use of an app treating SAD. Study results are pivotal to future treatment application in SAD.
Generation and comprehensive analysis of Synechococcus elongatus–Aspergillus nidulans co-culture system for polyketide production
Authors: Feng Jie , Li Jingwei , Liu Dongxia , Xin Yuxian , Sun Jingrong , Yin Wen-Bing , Li Tingting 
Published: March 2023

Abstract
Background
Artificial microbial consortia composed of heterotrophic and photoautotrophic organisms represent a unique strategy for converting light energy and carbon dioxide into high-value bioproducts. Currently, the types of desired bioproducts are still limited, and microbial fitness benefit rendered by paired partner generally needs to be intensified. Exploring novel artificial microbial consortia at a laboratory scale is an essential step towards addressing this unmet need. This study aimed to conduct and analyze an artificial consortium composed of cyanobacterium Synechococcus elongatus FL130 with the filamentous fungus Aspergillus nidulans TWY1.1 for producing fungi-derived secondary metabolite of polyketide neosartoricin B.

Results
Polyketide-producing A. nidulans TWY1.1 substantially ameliorated the growth and the survival of sucrose-secreting cyanobacterium S. elongatus FL130 in salt-stressed environments. Besides sucrose, comparable amounts of other carbohydrates were released from axenically cultured FL130 cells, which could be efficiently consumed by TWY1.1. Relative to axenically cultured FL130, less glycogen was accumulated in FL130 cells co-cultured with TWY1.1, and the glycogen phosphorylase gene catalyzing the first step for glycogen degradation had two-fold expression. Different from axenically cultured filamentous fungi, abundant vacuoles were observed in fungal hyphae of TWY1.1 co-cultured with cyanobacterium FL130. Meanwhile, FL130 cells displayed a characteristic pattern of interacting with its heterotrophic partner, densely dispersing along certain hyphae of TWY1.1. Finally, polyketide neosartoricin B was produced from TWY1.1 in FL130-TWY1.1 co-cultures, which was tightly adjusted by nitrogen level.

Conclusion
Overall, the results thoroughly proved the concept of pairing cyanobacteria with filamentous fungi to build artificial consortia for producing fungi-derived biomolecules.
Interplay between chronic inflammation and clonal haematopoiesis of indeterminate potential in Behçet’s disease
Authors: Park Jihye , An Hongyul , Lim Jiwoo , Park I Seul , Kim Mi Hyun , Kim Ji Hyung , Kim Seung Won , Koh Young Il , Lee Eun Young , Cheon Jae Hee 
Published: March 2023

Abstract
Background
Clonal haematopoiesis of indeterminate potential (CHIP) is a predisposition to haematological malignancy whose relationship with chronic inflammatory diseases, such as cardiovascular diseases, has been highlighted. Here, we aimed to investigate the CHIP emergence rate and its association with inflammatory markers in Behçet’s disease (BD).

Methods
We performed targeted next-generation sequencing to detect the presence of CHIP using peripheral blood cells from 117 BD patients and 5004 healthy controls between March 2009 and September 2021 and analysed the association between CHIP and inflammatory markers.

Results
CHIP was detected in 13.9% of patients in the control group and 11.1% of patients in the BD group, indicating no significant intergroup difference. Among the BD patients of our cohort, five variants (DNMT3A, TET2, ASXL1, STAG2, and IDH2) were detected. DNMT3A mutations were the most common, followed by TET2 mutations. CHIP carriers with BD had a higher serum platelet count, erythrocyte sedimentation rate, and C-reactive protein level; older age; and lower serum albumin level at diagnosis than non-CHIP carriers with BD. However, the significant association between inflammatory markers and CHIP disappeared after the adjustment for various variables, including age. Moreover, CHIP was not an independent risk factor for poor clinical outcomes in patients with BD.

Conclusions
Although BD patients did not have higher CHIP emergence rates than the general population, older age and degree of inflammation in BD were associated with CHIP emergence.
Is vitamin B12 deficiency a risk factor for gastroparesis in patients with type 2 diabetes?
Authors: Ahmed Sally S. , El-Hafez Hala A. Abd , Mohsen Mohamed , El-Baiomy Azza A. , Elkhamisy Enas T. , El-Eshmawy Mervat M. 
Published: March 2023

Abstract
Background
Diabetic gastroparesis is a severe diabetic complication refers to delayed gastric emptying in the absence of mechanical obstruction of the stomach. Vitamin B12 affects the dynamics of autonomic nervous system and its deficits has been linked to cardiovascular autonomic neuropathy therefore, vitamin B12 deficiency was hypothesized to be implicated in the development of diabetic gastroparesis. This study was conducted to explore the possible association between vitamin B12 deficiency and gastroparesis in patients with type 2 diabetes (T2D).

Methods
A total of 100 T2D patients with diabetes duration > 10 years and 50 healthy controls matched for age and sex were recruited for this study. T2D patients were divided into 2 groups: patients with gastroparesis and patients without gastroparesis. The diagnosis of gastroparesis was based on Gastroparesis Cardinal Symptom Index (GCSI) Score ≥ 1.9 and ultrasonographic findings including gastric emptying ˂ 35.67% and motility index ˂ 5.1. Anthropometric measurements, plasma glucose, glycosylated hemoglobin (HbA1c), lipids profile, vitamin B12 and transabdominal ultrasonography were assessed.

Results
The frequency of vitamin B12 deficiency in total patients with T2D was 35% (54.5% in patients with gastroparesis vs. 11.1% in patients without gastroparesis, P < 0. 001). Vitamin B12 level was negatively correlated with GCSI Score whereas, it was positively correlated with gastric emptying and motility index. Vitamin B12 deficiency was an independent predictor for gastroparesis in patients with T2D; it predicts gastroparesis at a cut off value of 189.5 pmol/L with 69.1% sensitivity and 64.4% specificity, P = 0.002.

Conclusions
Beside the known risk factors of diabetic gastroparesis, vitamin B12 deficiency is an independent predictor of diabetic gastroparesis in patients with T2D.
High quality, high reliability: The dynamics of camel milk marketing in northern Kenya
Authors: Nori Michele 
Published: March 2023

AbstractIn order to analyse the transformations faced by pastoralists in coping with the uncertainties of their livelihoods, this paper analyses the evolutions of the marketing of camel milk, which has turned from a local taboo into a critical asset for the pastoral communities of Isiolo, a county typically associated with the rearing of cattle. Camel milk marketing (CMM) challenges several assumptions about pastoral societies and their supposed embedding conservativeness, inefficiency and risk aversion.A high-reliability perspective has been instrumental in revealing the ongoing dynamics that are reconfiguring pastoral resource management and livelihood patterns in Isiolo. Through this approach, the management practices and contractual relationships that underpin CMM production and marketing have been explored, with a view to unveiling the rationale inspiring the networks through which camel milk is transacted, and the related socially-regulated arrangements and practices. The social and political embeddedness of CMM networks is central to their ability to adapt and function in constantly changing local conditions.The marketing of camel milk provides an intriguing insight into how pastoral systems are informed by a high-reliability approach to operating under conditions of volatility and uncertainty. Political economy matters will have a significant influence on the evolution of this important enterprise. Adequate investments in infrastructure and public facilities would be crucial to further develop CMM and to enable its operators to respond and adapt to local dynamics, with a view to fairly share the associated risks, costs and benefits.
“…he’s going to be facing the same things that he faced prior to being locked up”: perceptions of service needs for substance use disorders
Authors: Beeler Sara , Renn Tanya , Pettus Carrie 
Published: March 2023

AbstractBackgroundHigh rates of substance use disorders (SUDs) exist among justice-involved populations (i.e., persons incarcerated or recently released). SUD treatment is crucial for justice-involved populations as unmet treatment need increases reincarceration risk and impacts other behavioral health sequalae. A limited understanding of health needs (i.e. health literacy) can be one reason for unmet treatment needs. Social support is critical to seeking SUD treatment and post-incarceration outcomes. However, little is known about how social support partners understand and influence SUD service utilization among formerly incarcerated persons.MethodsThis mixed method, exploratory study utilized data from a larger study comprised of formerly incarcerated men (n = 57) and their selected social support partners (n = 57) to identify how social support partners understand the service needs of their loved ones recently released from prison who returned to the community with a diagnosis of a SUD. Qualitative data included 87 semi-structured interviews with the social support partners covering post-release experiences with their formerly incarcerated loved one. Univariates were conducted on the quantitative service utilization data and demographics to complement the qualitative data.ResultsMajority of the formerly incarcerated men identified as African American (91%) averaging 29 years of age (SD = 9.58). Most social support partners were a parent (49%). Qualitative analyses revealed that most social support partners avoided using or did not know the language to use regarding the formerly incarcerated person’s SUD. Treatment needs were often attributed to focus on peer influences and spending more time at their residence/housing. Analyses did reveal that when treatment needs were recommended in the interviews, social support partners reported employment and education services to be most needed for the formerly incarcerated person. These findings align with the univariate analysis with their loved ones reporting employment (52%) and education (26%) as their most reported service utilized post-release, compared to only 4% using substance abuse treatment.ConclusionResults provide preliminary evidence suggesting social support partners do influence the types of services accessed by formerly incarcerated persons with SUD. The findings of this study emphasize the need for psychoeducation during and after incarceration for individuals with SUDs and their social support partners.
Short-term photovoltaic power production forecasting based on novel hybrid data-driven models
Authors: Alrashidi Musaed , Rahman Saifur 
Published: March 2023

AbstractThe uncertainty associated with photovoltaic (PV) systems is one of the core obstacles that hinder their seamless integration into power systems. The fluctuation, which is influenced by the weather conditions, poses significant challenges to local energy management systems. Hence, the accuracy of PV power forecasting is very important, particularly in regions with high PV penetrations. This study addresses this issue by presenting a framework of novel forecasting methodologies based on hybrid data-driven models. The proposed forecasting models hybridize Support Vector Regression (SVR) and Artificial Neural Network (ANN) with different Metaheuristic Optimization Algorithms, namely Social Spider Optimization, Particle Swarm Optimization, Cuckoo Search Optimization, and Neural Network Algorithm. These optimization algorithms are utilized to improve the predictive efficacy of SVR and ANN, where the optimal selection of their hyperparameters and architectures plays a significant role in yielding precise forecasting outcomes. In addition, the proposed methodology aims to reduce the burden of random or manual estimation of such paraments and improve the robustness of the models that are subject to under and overfitting without proper tuning. The results of this study exhibit the superiority of the proposed models. The proposed SVR models show improvements compared to the default SVR models, with Root Mean Square Error between 12.001 and 50.079%. Therefore, the outcomes of this research work can uphold and support the ongoing efforts in developing accurate data-driven models for PV forecasting.
Antifungal activity and application of Bacillus tequilensis A13 in biocontrol of Rehmannia glutinosa root-rot disease
Authors: Wang Ruifei , Li Haibing , Qin Zhao , Wang Yan , Yang Qingxiang , Zhang Hao , Li Mingjun 
Published: March 2023

Abstract
Background
The bacterial genus Bacillus, an important group of bacteria which can suppress phytopathogens, has been widely used in agriculture. However, different species of Bacillus often displayed significant differences in probiotic efficiency and mechanism, suggesting that it is very necessary to investigate the biocontrol potential of new Bacillus isolates, especially from under-evaluated Bacillus spp. Rehmannia glutinosa (R. glutinosa), an important traditional Chinese medicinal herb, is affected by a serious root-rot disease caused by the fungus Fusarium solani (F. solani). Biocontrol agents against this root-rot disease in R. glutinosa have yet to be developed. This study explored, for the first time, the activity and biocontrol mechanism of a new Bacillus isolate, Bacillus tequilensis A13, in antagonizing F. solani.

Results
B. tequilensis A13 displayed a strong inhibitory activity (73.49% ± 1.33%) against F. solani growth in vitro and was able to survive and multiply in the sterilized soil. The results from liquid chromatography electrospray ionization tandem mass spectrometry (LC–MS/MS) and Gene Ontology (GO) analyses indicated that B. tequilensis A13 cell-free supernatant contained six antifungal compounds, eight antifungal compound synthases, and several functional proteins involved in the processes of plant stress resistance, etc. Finally, the joint application of complex fertilizer together with B. tequilensis A13 significantly reduced the incidence of R. glutinosa root rot in the field.

Conclusion
B. tequilensis A13 strongly inhibited F. solani growth by producing antifungal compounds and proteins associated with plant stress resistance/tolerance, and proved to be a promising candidate biocontrol agent against R. glutinosa root-rot disease.

Graphical Abstract
A systematic review of Mobile-Assisted Vocabulary Learning research
Authors: Okumuş Dağdeler Kübra 
Published: March 2023

AbstractWith rapid development of mobile technology, the number of researches on its use in language education process has increased. This growing body of interest has led to a need for review studies that are expected to explain the literature and the trend in the field succinctly. Based on this ground, this study aimed to review the studies conducted on mobile-assisted vocabulary learning in two dimensions. Firstly, it tried to reveal a general map of the domain through bibliometric analysis. With this aim, Web of Science database was scanned and 687 publications were included in the analysis. Then, among these publications 19 articles were selected to identify the technological and pedagogical focus of research papers to determine the trend. The results of bibliometric analysis explained the most frequent keywords, the most productive countries, and the most influential authors and articles. Furthermore, it was discovered that achievement and perceptions were the most common topics in terms of language learning focus and it was gamification in the dimension of technological focus in empirical studies. Despite its preliminary character, the findings have provided insights for future studies.