bluuebunny/crossref_metadata_2025_split huggingface dataset:
Konsep Penguatan Kepatuhan Masyarakat Terhadap Isolasi Mandiri Berbasis Pemberdayaan dan Partisipasi Masyarakat
Authors: Sutrisno Sutrisno , Nur Djannah Sitti , Solikhah Solikhah 
Published: May 2023

Self-isolation is one of the efforts of the Indonesian Ministry of Health in handling the spread of Covid-19. This study aims to describe the behaviour of the community on compliance with the implementation of health protocols, especially related to self-isolation and to design a strategy for strengthening community compliance in self-isolation based on community empowerment and participation. The research was conducted using a qualitative method with a phenomenological approach. The research was conducted at Bumi Mulia Housing Estate, Semarangan, Sidokarto, Godean, Sleman, Yogyakarta. The participants involved in this study were all residents of the Bumi Mulia housing estate. Problems are identified through the measurement of community knowledge and behaviour, then followed up by making a conceptual framework and program of activities so that the community can jointly participate in participatory efforts to overcome the problems that have been identified in the previous stage. There are still problems related to community knowledge and behaviour in terms of self-isolation. Knowledge and attitudes that are not yet good in this case will affect the behaviour of the community regarding compliance with health protocols, especially related to self-isolation. Empowerment and community participation can be used as a strategy model in strengthening community groups to face health problems through community participation. With community empowerment and participation, people will be able to solve problems more effectively, independently, and organized to create conditions for people who are more prepared to face disasters. Keywords: Self-isolation, covid-19, community empowerment
MAPPING HYPERTENSION DISEASE IN WOMEN AGED 25-45 YEARS AT TLOGOSARI KULON PRIMARY HEALTH CARE IN 2019 AND 2020
Authors: Farma Trianaldha Avina , Suharyo Suharyo 
Published: May 2023

Based on SIRANDU Semarang City, Tlogosari Kulon Primary Health Care (PHC) in 2019 had hypertension in the fifth position which reached 1.835 cases, and in 2020 it took third place that reached 3.091 cases. This study aims to find out the spread of hypertensive disease in women aged 25-45 years at Tlogosari Kulon PHC in 2019 and 2020. This study was a descriptive study that used an observation method with the entire population was hypertension case report in Tlogosari Kulon PHC in 2019 and 2020. The data source was from Morbidity Monthly Report (LB 1). The data was processed into new information using QGIS software. The results stated that in 2019 based on the village there were 100 cases, and in 2020 there were 221 cases. Meanwhile, compared to the number of female residents in 2019 the ratio was 0.22% or 100 out of 46.202 female residents. While in 2020 it reached 0.48% or 221 out of 46.202. In the results known that Muktiharjo Kidul Village was the area with the most amount of hypertension cases based on the village and the number of female residents. In presenting and reporting disease, it is better to use a geographic information system to find out a large number of people with hypertension. It can make it easier for PHC to prioritized the area and promoting health to maintain a healthy lifestyle so that people can avoid all risks of hypertension. It’s necessary to further research about the effect of the population on the incidence of hypertension. Keywords: Mapping, Hypertension, Women Aged 25-45 years Literature : 21 (2009-2020)
LITERATURE REVIEW: “ANALISIS IMPLEMENTASI GERAKAN MASYARAKAT CERDAS MENGGUNAKAN OBAT”
Authors: Chrisdita Maya , Suryoputro Antono , Asro Septo Pawelas 
Published: May 2023

Masalah kesehatan yang sering dialami masyarakat sekarang ini mendorong masyarakat untuk melakukan pengobatan sendiri (Swamedikasi) sebanyak 66% berdasarkan Survey Sosial Ekonomi Nasional (SUSENAS). Swamedikasi yang tidak tepat dapat merugikan bagi kesehatan sehingga perlu adanya edukasi dan sosialisasi tentang Gerakan Masyarakat Cerdas Menggunakan Obat (GeMa CerMat). Tujuan Gema Cermat ialah masyarakat mampu memilih obat yang tepat sesuai dengan penggunaan obat yang benar serta memahami penyimpanan obat maupun pembuangan obat dengan benar. Tinjauan pustaka ini bertujuan untuk mengetahui implementasi gema cermat yang tepat bagi masyarakat. 7 Artikel yang kami dapatkan selama tahun 2019 hingga 2021 di google scholar menggunakan Program Gema Cermat dapat diberikan kepada masyarakat melalui berbagai cara seperti Community Based Interactive Approach (CBIA), Model pemberdayaan masyarakat partisipatif atau Participatory Rural Apraisal (PRA), edukasi melalui brosur, buku saku penggunaan dan penggolongan obat. Implementasi dari kegiatan Sosialisasi Gema Cermat berpengaruh terhadap pengetahuan responden, penilaian perilaku responden. Edukasi GEMA CERMAT dapat meningkatkan pengetahuan masyarakat tentang swamedikasi.
PATIENT CLAIM FINANCING AT RSUP DR. KARIADI SEMARANG DURING THE COVID-19 PANDEMIC
Authors: Agiwahyuanto, S.Kep.,M.Kes Faik , Widianawati Evina , Wulan Widya Ratna , Ayusasmita Via , Setiadi Deddy 
Published: May 2023

RSUP Dr. Kariadi Semarang sees both outpatients and inpatients to assess their health status and condition. Patient's health status and condition are classified into two groups: comorbid and co-incident. Patient claims are funded based on status and health conditions of comorbid and co-incident patients. The objectives this research is to know about the patient claim financing procedures of RSUP Dr. Kariadi Semarang during covid-19 pandemic. This research is descriptive qualitative using phenomenological. Primary data sources quotations were chosen using either purposeful sampling or criterion-based selection. The research informants are the primary informants. Informants are Hospital's first primary informant, the Coordinator of Coding Casemixes, and the second primary informant, the Nurse Coordinator of the Covid-19 Handling Room, as well as the first triangulation informant, the Head of the Medical Record Unit, and the second triangulation informant, the Head of the Covid-19 Handling Task Force. Interviews is methods for gathering data. Source triangulation is used for data triangulation. Data analysis using flow model of analysis and interactive analysis model. The result of this research are covid-19 with comorbidity or covid-19 with co-incident in patients because the guarantor is different, the case will be entered twice if it is a co-incident. If the condition is comorbid, the claim is handled separately. Previously, a claim was made for the covid-19 case, and if the covid-19 case was completed but the comorbidities persisted, the guarantor was changed from the Ministry of Health to BPJS Health. The conclusion is during the covid-19 pandemic, the procedures of RSUP Dr. Kariadi Semarang's patient claim financing process are two, such as the guarantor from the Ministry of Health and BPJS Health. Keywords: Patient claim, financing, process
ANALISIS PELAKSANAAN KESELAMATAN PASIEN TERKAIT AKREDITASI DI PUSKESMAS (LITERATURE REVIEW)
Authors: Hardy Bernadeta Lintang , Jati Sutopo Patria , Setyaningsih Yuliani 
Published: May 2023

Puskesmas as a first-level health facility must improve the quality of health services to the maximum so that the accreditation status increases and public trust to carry out health services also increases. One of the important indicators in improving the quality of health services at Puskesmas is patient safety. However, in Indonesia, the development of issues regarding patient safety at the Puskesmas level is not as dynamic as at the hospital level. This study aims to dig deeper and synthesize the scientific literature on patient safety and its relation to accreditation in health centers. This review uses 2 databases namely Google Scholar and Garuda. The main keywords are "Patient Safety in Puskesmas", "Quality of Service" "Accreditation". In conclusion, there are still many health workers who are less compliant and lack high commitment regarding patient safety, not all officers attend training related to patient safety, facilities and infrastructure that are less supportive, lack of funds, and policies that are not well implemented and realized. Keywords: Patient Safety in Puskesmas, Quality of Service, Accreditation.
Differences in Levels of Knowledge and Attitudes about Reproductive Health among Students of the Faculty of Health and Non-Health Faculties in Semarang City
Authors: Aprianti Aprianti , Wulandari Respati , Waluyo Dwi Eko 
Published: May 2023

Background: Adolescence is a period that is vulnerable to risky sexual behavior. This study aimed to describe the reproductive health knowledge and attitudes of students at the faculty of health and non-health faculties.Methods: It is research with quantitative methods using a cross-sectional design. The variables of this study are the level of knowledge and attitudes about reproductive health—data collection techniques with online surveys. Data analysis used descriptive statistics and bivariate analysis (Mann-Whitney test).Results: Respondents with a good level of knowledge and attitudes about sexual reproductive health at the health faculties were higher than respondents at the non-health faculties (78 Vs. 61 people and 68 Vs. 42 people). The knowledge and attitudes of respondents in the health and non-health faculties were significantly different (?=19; p = 0.009 and ?=27.95; p = 0.001).Conclusion: Health promotion is needed for students, especially non-health students, to increase knowledge and attitudes about reproductive health to prevent risky sexual behavior among students
A new portable toluidine blue/aptamer complex-on-polyethyleneimine-coated gold nanoparticles-based sensor for label-free electrochemical detection of alpha-fetoprotein
Authors: Yaiwong Patrawadee , Anuthum Siriporn , Sangthong Padchanee , Jakmunee Jaroon , Bamrungsap Suwussa , Ounnunkad Kontad 
Published: May 2023

The quantification of alpha-fetoprotein (AFP) as a potential liver cancer biomarker which is generally found in ultratrace level is of significance in biomedical diagnostics. Therefore, it is challenging to find a strategy to fabricate a highly sensitive electrochemical device towards AFP detection through electrode modification for signal generation and amplification. This work shows the construction of a simple, reliable, highly sensitive, and label-free aptasensor based on polyethyleneimine-coated gold nanoparticles (PEI-AuNPs). A disposable ItalSens screen-printed electrode (SPE) is employed for fabricating the sensor by successive modifying with PEI-AuNPs, aptamer, bovine serum albumin (BSA), and toluidine blue (TB), respectively. The AFP assay is easily performed when the electrode is inserted into a small Sensit/Smart potentiostat connected to a smartphone. The readout signal of the aptasensor derives from the electrochemical response of TB intercalating into the aptamer-modified electrode after binding with the target. The decrease in current response of the proposed sensor is proportional to the AFP concentration due to the restriction of the electron transfer pathway of TB by a number of insulating AFP/aptamer complexes on the electrode surface. PEI-AuNPs improve SPE’s reactivity and provide a large surface area for aptamer immobilization whereas aptamer provides selectivity to the target AFP. Consequently, this electrochemical biosensor is highly sensitive and selective for AFP analysis. The developed assay reveals a linear range of detection from 10 to 50000 pg mL−1 with R2 = 0.9977 and provided a limit of detection (LOD) of 9.5 pg mL−1 in human serum. With its simplicity and robustness, it is anticipated that this electrochemical-based aptasensor will be a benefit for the clinical diagnosis of liver cancer and further developed for other biomarkers analysis.
Encapsulation of β-NGF in injectable microrods for localized delivery accelerates endochondral fracture repair
Authors: Rivera Kevin O. , Cuylear Darnell L. , Duke Victoria R. , O’Hara Kelsey M. , Zhong Justin X. , Elghazali Nafisa A. , Finbloom Joel A. , Kharbikar Bhushan N. , Kryger Alex N. , Miclau Theodore , Marcucio Ralph S. , Bahney Chelsea S. , Desai Tejal A. 
Published: May 2023

Introduction: Currently, there are no non-surgical FDA-approved biological approaches to accelerate fracture repair. Injectable therapies designed to stimulate bone healing represent an exciting alternative to surgically implanted biologics, however, the translation of effective osteoinductive therapies remains challenging due to the need for safe and effective drug delivery. Hydrogel-based microparticle platforms may be a clinically relevant solution to create controlled and localized drug delivery to treat bone fractures. Here, we describe poly (ethylene glycol) dimethacrylate (PEGDMA)-based microparticles, in the shape of microrods, loaded with beta nerve growth factor (β-NGF) for the purpose of promoting fracture repair.Methods: Herein, PEGDMA microrods were fabricated through photolithography. PEGDMA microrods were loaded with β-NGF and in vitro release was examined. Subsequently, bioactivity assays were evaluated in vitro using the TF-1 tyrosine receptor kinase A (Trk-A) expressing cell line. Finally, in vivo studies using our well-established murine tibia fracture model were performed and a single injection of the β-NGF loaded PEGDMA microrods, non-loaded PEGDMA microrods, or soluble β-NGF was administered to assess the extent of fracture healing using Micro-computed tomography (µCT) and histomorphometry.Results:In vitro release studies showed there is significant retention of protein within the polymer matrix over 168 hours through physiochemical interactions. Bioactivity of protein post-loading was confirmed with the TF-1 cell line. In vivo studies using our murine tibia fracture model show that PEGDMA microrods injected at the site of fracture remained adjacent to the callus for over 7 days. Importantly, a single injection of β-NGF loaded PEGDMA microrods resulted in improved fracture healing as indicated by a significant increase in the percent bone in the fracture callus, trabecular connective density, and bone mineral density relative to soluble β-NGF control indicating improved drug retention within the tissue. The concomitant decrease in cartilage fraction supports our prior work showing that β-NGF promotes endochondral conversion of cartilage to bone to accelerate healing.Discussion: We demonstrate a novel and translational method wherein β-NGF can be encapsulated within PEGDMA microrods for local delivery and that β-NGF bioactivity is maintained resulting in improved bone fracture repair.
Patient-derived organoids as a platform for drug screening in metastatic colorectal cancer
Authors: He Xingfeng , Jiang Yan , Zhang Long , Li Yaqi , Hu Xiang , Hua Guoqiang , Cai Sanjun , Mo Shaobo , Peng Junjie 
Published: May 2023

Introduction: Most advanced colorectal cancers are aggressive, and there is a lack of effective methods for selecting appropriate anticancer regimens. Patient-derived organoids (PDOs) have emerged as preclinical platforms for modeling clinical responses to cancer therapy.Methods: In this study, we successfully constructed a living biobank with 42 organoids derived from primary and metastatic lesions of metastatic colorectal cancer patients. Tumor tissue was obtained from patients undergoing surgical resection of the primary or metastatic lesion and then used to establish PDOs. Immunohistochemistry (IHC) and drug sensitivity assays were performed to analyze the properties of these organoids.Results: The mCRC organoids were successfully established with an 80% success rate. The PDOs maintained the genetic and phenotypic heterogeneity of their parental tumors. The IC50 values of5-fluorouracil (5-FU), oxaliplatin, and irinotecan (CPT11) were determined for mCRC organoids using drug sensitivity assays. The in vitro chemosensitivity data revealed the potential value of PDOs for clinical applications in predicting chemotherapy response and clinical outcomes in mCRC patients.Discussion: In summary, the PDO model is an effective platform for in vitro assessment of patient-specific drug sensitivity, which can guide personalized treatment decisions for patients with end-stage CRC.
Material quantities and estimated construction costs for new elevated IRC 2015-compliant single-family home foundations
Authors: Kodavatiganti Yamini , Rahim Md Adilur , Friedland Carol J. , Mostafiz Rubayet Bin , Taghinezhad Arash , Heil Shandy 
Published: May 2023

Residential foundation selection is critical for the lifetime performance of the project, but the type of foundation selected can depend on cost. This study aims to compare the new construction costs of residential slab-on-fill and four types of crawl space foundations for residential projects by quantifying the material quantities for each and exploring the effect of building characteristics on material quantities and total costs. The building characteristics selected are size (139 m2, 186 m2, and 232 m2), footprint aspect ratio (1:1, 1:2.5, and 1:5) and first floor elevation (0 m–1.2 m in 0.3 m increments). We find that material quantities and total costs change not only with building size and elevation, but also with footprint aspect ratio. Applying 2022 RSMeans Building Construction Cost data, the results show that for any building size, aspect ratio, or elevation, raised wood flooring on pier foundations ($118–$180 per m2) and slab on fill ($103–$211 per m2) are the least expensive, while crawl space foundations with stemwalls are the most expensive ($147–$280 per m2). The results of this study can be used by residential builders, developers and designers in evaluating foundation costs and design alternatives. The methodology may also be adapted for use in other applications such as flood mitigation or energy efficiency projects or environmental sustainability assessments.
Single-cell analysis reveals dysregulated inflammatory response in peripheral blood immunity in patients with acute respiratory distress syndrome
Authors: Mo Jingjia , Yang Yanli , Feng Jihua , Lei Yanhua , Huang Suhong , Cen Weiluan , Wei Shanshan , Huang Hao , Lu Junyu , Zhang Jianfeng 
Published: May 2023

Introduction: Acute respiratory distress syndrome (ARDS) remains a major clinical challenge for patients in intensive care units. Determining the differential mechanisms underlying ARDS with different etiologies is a key goal to improve the effectiveness of ARDS therapy. Despite growing evidence that different immune cell types are involved in ARDS, the role of altered immune cell subpopulations in disease progression is unelucidated.Methods: In this study, we combined scRNA-seq and bulk-level sequencing to analyze the transcriptomes of peripheral blood mononuclear cells from healthy volunteers and patients with septic ARDS (sep-ARDS) and pneumonic ARDS (PNE-ARDS).Results: Our data revealed differential alterations at the cellular and molecular levels and within biological signaling pathways in ARDS with different etiologies. The dynamics of neutrophils, macrophages (Macs), classical dendritic cells (cDCs), myeloid-derived suppressive cells (MDSCs), and CD8+ T cells varied significantly among groups of different samples, with neutrophils and cDCs at higher, and Macs at significantly lower, amounts in the patients with sep-ARDS. Furthermore, MDSCs were highly enriched only in the sep-ARDS patients, whereas a higher abundance of CD8+ T cells was observed in patients with PNE-ARDS. In addition, these cell subpopulations were found to be significantly involved in apoptosis, inflammatory, and immune-related pathways. In particular, a significant enhancement of the oxidative stress response was observed in the neutrophil subpopulation.Conclusion: Our study shows that the composition of cells involved in the main peripheral circulation differs in patients with ARDS with different etiologies. Studying the role and mechanism of action of these cells during ARDS will provide new opportunities for the treatment of this condition.
Identifying promising GSK3β inhibitors for cancer management: a computational pipeline combining virtual screening and molecular dynamics simulations
Authors: Hua Libo , Anjum Farah , Shafie Alaa , Ashour Amal Adnan , Almalki Abdulraheem Ali , Alqarni Ali Abdullah , Banjer Hamsa Jameel , Almaghrabi Sarah Abdullah , He Shan , Xu Nenggui 
Published: May 2023

Glycogen synthase kinase-3 (GSK3β), a serine/threonine protein kinase, has been discovered as a novel target for anticancer drugs. Although GSK3β is involved in multiple pathways linked to the etiology of various cancers, no specific GSK3β inhibitor has been authorized for cancer therapy. Most of its inhibitors have toxicity effects therefore, there is a need to develop safe and more potent inhibitors. In this study, a library of 4,222 anti-cancer compounds underwent rigorous computational screening to identify potential candidates for targeting the binding pocket of GSK3β. The screening process involved various stages, including docking-based virtual screening, physicochemical and ADMET analysis, and molecular dynamics simulations. Ultimately, two hit compounds, BMS-754807 and GSK429286A, were identified as having high binding affinities to GSK3β. BMS-754807 and GSK429286A exhibited binding affinities of −11.9, and −9.8 kcal/mol, respectively, which were greater than that of the positive control (−7.6 kcal/mol). Further, molecular dynamics simulations for 100 ns were employed to optimize the interaction between the compounds and GSK3β, and the simulations demonstrated that the interaction was stable and consistent throughout the study. These hits were also anticipated to have good drug-like properties. Finally, this study suggests that BMS-754807 and GSK429286A may undergo experimental validation to evaluate their potential as cancer treatments in clinical settings.
Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020
Authors: Xiao Ruiqi , Li Ying , Liu Xiaowei , Ding Yijun , Lai Jidong , Li Yangfang , Kang Wenqing , Zou Peicen , Wang Jie , Du Yue , Zhang Jinjing , Wang Yajuan 
Published: May 2023

BackgroundEscherichia coli is one of the most common pathogens causing neonatal infections. Recently, the incidence and drug resistance of E. coli have increased, posing a major threat to neonatal health. The aim of this study was to describe and analyze the antibiotic resistance and multilocus sequence typing (MLST) characteristics of E. coli derived from infants admitted to neonatal intensive care units (NICUs) across China.MethodsIn this study, 370 strains of E. coli from neonates were collected. E. coli isolated from these specimens were subjected to antimicrobial susceptibility testing (by broth microdilution method) and MLST.ResultsThe overall resistance rate was 82.68%, with the highest rate of methicillin/sulfamethoxazole (55.68%) followed by cefotaxime (46.22%). Multiple resistance rate was 36.74%, 132 strains (35.68%) had extended-spectrum β-lactamase (ESBL) phenotype and 5 strains (1.35%) had insensitivity to the tested carbapenem antibiotics. The resistance of E. coli isolated from different pathogenicity and different sites of infections varied, strains derived from sputum were significantly more resistant to β-lactams and tetracyclines. Currently, the prevalence spectrum in NICUs was dominated by ST1193, ST95, ST73, ST69 and ST131 across China. And the multidrug resistance of ST410 was the most severe. ST410 had the highest resistance rate to cefotaxime (86.67%), and its most common multidrug resistance pattern was β-lactams + aminoglycosides + quinolones + tetracyclines + sulfonamides.ConclusionsSubstantial proportions of neonatal E. coli isolates were severely resistant to commonly administered antibiotics. MLST results can suggest the prevalent characteristics of antibiotic resistance in E. coli with different ST types.