bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancement of microwave absorption performance of porous carbon induced by Ce (CO3) OH
Authors: Wang Jijun , Chen Yuhua , Wei Yaxing , Li Yan , Li Fangyuan , Li Bingzhen , Wu Qingqing , Zhao Jinlong 
Published: January 2023

In recent years, electromagnetic pollution has become more and more serious, resulting in a very negative impact on people’s health. Therefore, it is important to develop efficient microwave absorbers to reduce electromagnetic pollution. Here, we construct a novel absorbing material of the polymer gel-derived porous carbon decorated by rare earth compounds (Ce (CO3) OH). When the thickness is 2.2 mm, the composite exhibits excellent microwave absorption performance with the optimal RLmin value and EAB reached up to −47.67 dB and 5.52 GHz, respectively, covering the Ku band. The high-efficiency microwave absorption is mainly attributed to the synergistic effect of dipole polarization, defect polarization and interfacial polarization. This work not only provides a new view for designing superior absorber materials, but also lay a foundation for their real applications.
Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children
Authors: Chen Yinghu , Mao Lingfeng , Lai Dengming , Xu Weize , Zhang Yuebai , Wu Sihao , Yang Di , Zhao Shaobo , Liu Zhicong , Xiao Yi , Tang Yi , Meng Xiaofang , Wang Min , Shi Jueliang , Chen Qixing , Shu Qiang 
Published: January 2023

ObjectivesTo develop a rapid and low-cost method for 16S rDNA nanopore sequencing.MethodsThis was a prospective study on a 16S rDNA nanopore sequencing method. We developed this nanopore barcoding 16S sequencing method by adding barcodes to the 16S primer to reduce the reagent cost and simplify the experimental procedure. Twenty-one common pulmonary bacteria (7 reference strains, 14 clinical isolates) and 94 samples of bronchoalveolar lavage fluid from children with severe pneumonia were tested. Results indicating low-abundance pathogenic bacteria were verified with the polymerase chain reaction (PCR). Further, the results were compared with those of culture or PCR.ResultsThe turnaround time was shortened to 6~8 hours and the reagent cost of DNA preparation was reduced by employing a single reaction adding barcodes to the 16S primer in advance. The accuracy rate for the 21 common pulmonary pathogens with an abundance ≥ 99% was 100%. Applying the culture or PCR results as the gold standard, 71 (75.5%) of the 94 patients were positive, including 25 positive cultures (26.6%) and 52 positive quantitative PCRs (55.3%). The median abundance in the positive culture and qPCR samples were 29.9% and 6.7%, respectively. With an abundance threshold increase of 1%, 5%, 10%, 15% and 20%, the test sensitivity decreased gradually to 98.6%, 84.9%, 72.6%, 67.1% and 64.4%, respectively, and the test specificity increased gradually to 33.3%, 71.4%, 81.0%, 90.5% and 100.0%, respectively.ConclusionsThe nanopore barcoding 16S sequencing method can rapidly identify the pathogens causing bacterial pneumonia in children.
The successful treatment of Enterocytozoon bieneusi Microsporidiosis with nitazoxanide in a patient with B-ALL: A Case Report
Authors: Zhou Lanlan , Guan Zebing , Chen Chaolun , Zhu Qiuhua , Qiu Shiqiu , Liu Yanan , Li Mingjie , Zeng Wenbin , Wang Hong , Gao Yanmin , Yuan Yuemei , Zhang Hanling , Ruan Guanqiao , Pan Xueyi 
Published: January 2023

IntroductionEnterocytozoon bieneusi (E. bieneusi) Microsporidia can cause opportunistic infections in immunocompromised patients and is also an emerging disease in these individuals. Its clinical manifestations are chronic diarrhea and severe wasting syndrome, these can be extremely debilitating and carry a significant risk of death for immunocompromised patients. Often, microsporidia cannot be confirmed immediately by routine examination and culture. Effective and available treatment options are limited for infections caused by E. bieneusi in humans. Such cases are very rare in Chinese Mainland.Case presentationA 47-year-old male had recurrent, profuse watery diarrhea and abdominal discomfort for more than 7 months, with a fever for 5 days. Two years earlier, he received treatment with a modified BFM-90 protocol for acute B cell lymphoblastic leukemia and is currently in the final stages of maintenance therapy with oral methotrexate and mercaptopurine. The leukemia was assessed as still in remission two months ago. PET/CT showed massive peritoneal fluid accumulation and a high uptake area in the diffused peritoneum (SUVmax 12.57), suggesting tumor invasion or microbial infections. However, broad-spectrum antibacterial therapies were ineffective. Metagenomic sequencing of plasma and peritoneal fluid showed no suggestion of the existence of a tumor but instead showed a high sequence number of DNA and RNA of the Microsporidia. His albendazole treatment failed and subsequent treatment with nitazoxanide successfully resolved the infection.ConclusionThis case shows that we should consider the possibility of atypical pathogen infection in patients with hematologic malignancy who repeatedly develop unexplained diarrhea with wasting. mNGS can help rule out malignant neoplasms and diagnose infections. Our results suggest that nitazoxanide effectively treats E. bieneusi microsporidia infections.
The prognostic value of admission D-dimer level in patients with cardiogenic shock after acute myocardial infarction
Authors: Jiang Yi , Zhu Yuansong , Xiang Zhenxian , Sasmita Bryan Richard , Wang Yaxin , Ming Gong , Chen Siyu , Luo Suxin , Huang Bi 
Published: January 2023

BackgroundShock is associated with the activation of the coagulation and fibrinolytic system, and D-dimer is the degradation product of cross-linked fibrin. However, the prognostic value of D-dimer in patients with cardiogenic shock (CS) after acute myocardial infarction (AMI) remains unclear.MethodsWe retrospectively analyzed the data of consecutive patients with CS complicating AMI. The primary endpoint was 30-day mortality and the secondary endpoint was the major adverse cardiovascular events (MACEs) including 30-day all-cause mortality, ventricular tachycardia/ventricular fibrillation, atrioventricular block, gastrointestinal hemorrhage, and non-fatal stroke. Restricted cubic spline (RCS) analyses were performed to assess the association between admission D-dimer and outcomes. A multivariable Cox regression model was performed to identify independent risk factors. The risk predictive potency with D-dimer added to the traditional risk scores was evaluated by C-statistics and the net reclassification index.ResultsAmong 218 patients with CS complicating AMI, those who died during the 30-day follow-up presented with worse baseline characteristics and laboratory test results, including a higher level of D-dimer. According to the X-tile program result, the continuous plasma D-dimer level was divided into three gradients. The 30-day all-cause mortality in patients with low, medium, and high levels of D-dimer were 22.4, 53.3, and 86.2%, respectively (p &lt; 0.001 for all). The 30-day incidence of MACEs was 46.3, 77.0, and 89.7%, respectively (p &lt; 0.001). In the multivariable Cox regression model, the trilogy of D-dimer level was an independent risk predictor for 30-day mortality (median D-dimer cohort: HR 1.768, 95% CI 0.982–3.183, p = 0.057; high D-dimer cohort: HR 2.602, 95% CI 1.310–5.168, p = 0.006), a similar result was observed in secondary endpoint events (median D-dimer cohort: HR 2.012, 95% CI 1.329–3.044, p = 0.001; high D-dimer cohort: HR 2.543, 95% CI 1.452–4.453, p = 0.001). The RCS analyses suggested non-linear associations of D-dimer with 30-day mortality. The enrollment of D-dimer improved risk discrimination for all-cause death when combined with the traditional CardShock score (C-index: 0.741 vs. 0.756, pdifference = 0.004) and the IABP-SHOCK II score (C-index: 0.732 vs. 0.754, pdifference = 0.006), and the GRACE score (C-index: 0.679 vs. 0.715, pdifference &lt; 0.001). Similar results were acquired after logarithmic transformed D-dimer was included in the risk score. The improvements in reclassification which were calculated as additional net reclassification index were 7.5, 8.6, and 12.8%, respectively.ConclusionAdmission D-dimer level was independently associated with the short-term outcome in patients with CS complicating AMI and addition of D-dimer brought incremental risk prediction value to traditional risk prediction scores.
Region-dependent site conditions in China: Evidence from borehole data statistics
Authors: Yao Xinxin , Zhang Peng , Zhao Yu , Wang Hongwei , Wang Daren 
Published: January 2023

Consideration of site effects is vital in modeling and predicting earthquake ground motions. However, site conditions can vary markedly between different localities. The regional dependency of site conditions in China has not yet been investigated systematically. In this study, profiles of 6,179 boreholes were collected from four regions in China, i.e., the Capital Metropolitan (CM), Xinjiang (XJ), Guangdong—Guangxi (GG), and Sichuan–Yunnan (SY) areas. Quantitative characteristic parameters including site category, equivalent shear wave velocity (Vse), and the thickness of the overlying soil layer (H) defined by the Chinese seismic code, and VS30 and the median shear wave profile were all analyzed to confirm the dependency on site conditions among the four regions. Investigation revealed that the majority of sites in most regions are classified as Class-II sites with no Class-IV sites, except in CM. In comparison with the other three regions, a larger number of GG sites are classified as Class-I. Sites in XJ are generally characterized by small H values (&lt;20 m) and large Vse values (250 –450 m/s), while those in CM are characterized by large H values (average: ∼50 m) and small Vse values (150 –300 m/s). Generally, the Vse and H values are similar in GG and SY, i.e., the sites are covered by an overlying soil layer that is ∼20 m thick with an average Vse value of ∼250 m/s. A very thin (&lt;5 m) overlying soil layer is observed at many more sites in GG than at sites in the other three regions. The VS30 values in CM, SY, and GG all approximately follow a lognormal distribution with various logarithmic means, i.e., 257.2, 299.6, and 360.9 m/s, respectively. However, the VS30 values of most sites in XJ broadly follow a uniform distribution with a range of 200 –550 m/s. We summarized the characteristics of the median VS profiles of each VS30-based category (C1, C2, and C3) for each region. The median VS profiles between any two regions are mainly manifested as parallel trends for sites in category C1, whereas they present trends of intersection for sites in categories C2 and C3. The findings of this study could serve as a basis for the establishment of a regional empirical model for site-dependent seismic response in China.
Active learning to develop disciplinary competencies related to automatic control in engineering curricula using low cost do-it-yourself didactic stations
Authors: Navarro-Durán David , Félix-Herrán Luis C. , Membrillo-Hernández Jorge , Craig Kevin C. , Ramírez-Cadena Miguel J. , Ramírez-Mendoza Ricardo A. 
Published: January 2023

IntroductionIn general, in automatic control courses, the process of designing and testing a control system includes applying physical laws to model the system, working with virtual models, building one or various prototypes, and testing the control algorithms. However, in the industry, the approach must be more pragmatic because the design and implementation time must be shorter, and the success of the solution must be ensured.MethodsChallenged with this problem, a black-box model from which data are generated turns into a convenient starting point to design and implement the automation, and this approach is addressed in this research. The herein proposal is the design and implementation of didactic stations and their application in undergraduate automatic control courses. In the context of active learning, by using the stations to identify the model’s dynamics, and subsequently, design and implement an automatic system, students reinforce the theory and receive another stimulus for the development of competencies in automatic control.ResultsThe didactic stations emulate those cases in the industry where the hardware is already working, and it is necessary to automate or improve some process following a practical approach. During the first phase, students, guided by professors, designed and implemented four electromechanical prototypes. The second phase was using the prototypes in the curricular courses Control Engineering and Computerized Control to implement and evaluate controllers. The research included a control group and an experimental group. The group using the stations had a higher final course average grade than the control group.DiscussionThe findings encourage the application of this type of approach to complement the teaching of automatic control, which could positively impact the professional performance of future control engineers.
Comprehensive analysis of PTPN family expression and prognosis in acute myeloid leukemia
Authors: Liu Yong , Zhang Jing , Du Zefan , Huang Junbin , Cheng Yucai , Yi Wenfang , Li Tianwen , Yang Jing , Chen Chun 
Published: January 2023

Background: Tyrosyl phosphorylation is carried out by a group of enzymes known as non-receptor protein tyrosine phosphatases (PTPNs). In the current investigation, it is hoped to shed light on the relationships between the expression patterns of PTPN family members and the prognosis of acute myeloid leukemia (AML). Methods:PTPN expression was examined using GEPIA and GEO databases. To investigate the connection between PTPN expression and survival in AML patients, we downloaded data from the Broad TCGA Firehose and Clinical Proteomic Tumor Analysis (CPTAC) of the Cancer Genome Atlas (TCGA). We used quantitative real-time PCR (qRT-PCR) to confirm that essential genes were performed in clinical samples and cell lines. We then used western blot to verify that the genes expressed in the above databases were positive in normal tissues, AML patient samples, and AML cell lines. Next, we investigated associations between genome-wide expression profiles and PTPN6 expression using the GEO datasets. We investigated the interactive exploration of multidimensional cancer genomics using the cBioPortal datasets. Using the DAVID database, a study of gene ontology enrichment was performed. The protein-protein interaction (PPI) network was created using the STRING portal, and the gene-gene interaction network was performed using GeneMANIA. Results: Data from GEO and GEPIA revealed that most PTPN family members were linked to AML. Patients with leukemia have elevated levels of several PTPN members. All of the AML patients’ poor overall survival (OS, p &lt; .05) was significantly linked with higher expression of PTPN1, PTPN6, and PTPN7. Additionally, clinical samples showed that the expression of PTPN 6, PTPN 7, PTPN 13, and PTPN 14 was higher than normal in AML patients (p = .0116, p = .0034, p = .0092, and p = .0057, respectively) and AML cell lines (p = .0004, p = .0035, p = .0357, and p = .0177, respectively). Western blotting results showed that the expression of PTPN6 in AML samples and AML cell lines was significantly higher than that in normal control samples. Conclusion: Differentially expressed PTPN family members were found in AML. The prognosis of patients and PTPN gene expression were shown to be correlated. PTPN6 is one of these members and may be used as an AML diagnostic and prognostic marker.
Acute and chronic HBV infection in central Argentina: High frequency of sub-genotype F1b, low detection of clinically relevant mutations and first evidence of HDV
Authors: Castro Gonzalo M. , Sosa María J. , Sicilia Paola E. , Riberi María I. , Moreno Claudia , Cattaneo Rodolfo , Debes José D. , Barbás María G. , Cudolá Analía E. , Pisano María B. , Ré Viviana E. 
Published: January 2023

IntroductionGenomic analysis of hepatitis B virus (HBV) identifies phylogenetic variants, which may lead to distinct biological and clinical behaviors. The satellite hepatitis D virus (HDV) may also influence clinical outcomes in patients with hepatitis B. The aim of this study was to investigate HBV genetic variants, including clinically relevant mutations, and HDV infection in acute and chronic hepatitis B patients in central Argentina.MethodsA total of 217 adult HBV infected patients [acute (AHB): n = 79; chronic (CHB): n = 138] were studied; 67 were HBV/human immunodeficiency virus (HIV) coinfected. Clinical and demographic data were obtained from medical records. Serological markers were determined. Molecular detection of HBV and HDV was carried out by RT-Nested PCR, followed by sequencing and phylogenetic analysis.ResultsOverall, genotype (gt) F [sub-genotype (sgt) F1b] was the most frequently found. In AHB patients, the gts/sgts found were: F1b (74.7%) &gt; A2 (13.9%) &gt; F4 (7.6%) &gt; C (2.5%) &gt; A1 (1.3%). Among CHB patients: F1b (39.1%) &gt; A2 (23.9%) &gt; F4 (18.2%) &gt; D (9.4%) &gt; C and F6 (3.6% each) &gt; A1, A3 and B2 (0.7% each). The distribution of sgt A2 and gt D was significantly different between HBV mono and HBV/HIV coinfected patients [A2: 15.9% vs. 35.7% (p &lt; 0.05), respectively and D: 14.6% vs. 1.8% (p &lt; 0.05), respectively]. Mutation frequency in basal core promoter/pre-Core (BCP/pC) region was 35.5% (77/217) [AHB: 20.3% (16/79), CHB: 44.2% (61/138)]. In the open reading frame (ORF) S, mutations associated with vaccine escape and diagnostic failure were detected in 7.8% of the sequences (17/217) [AHB: 3.8% (3/79), CHB: 10.1% (14/138)]. ORF-P amino acid substitutions associated with antiviral resistance were detected in 3.2% of the samples (7/217) [AHB: 1.3% (1/79), CHB 4.3%, (6/138)]. The anti-HDV seropositivity was 5.2% (4/77); one sample could be sequenced, belonging to gt HDV-1 associated with sgt HBV-D3.DiscussionWe detected an increase in the circulation of genotype F in Central Argentina, particularly among AHB patients, suggesting transmission advantages over the other genotypes. A low rate of mutations was detected, especially those with antiviral resistance implications, which is an encouraging result. The evidence of HDV circulation in our region, reported for the first time, alerts the health system for its search and diagnosis.
Serum creatinine/cystatin C ratio as a muscle mass evaluating tool and prognostic indicator for hospitalized patients: A meta-analysis
Authors: Zheng Wen-He , Zhu Yi-Bing , Yao Yan , Huang Hui-Bin 
Published: January 2023

ObjectiveSarcopenia is a syndrome of decreased muscle mass and deficits in muscle strength and physical function. We aimed to investigate the relationship between creatinine/cystatin C ratio (CCR) and sarcopenia and the prognostic value of CCR in hospitalized patients.Materials and methodsWe searched for relevant studies in PubMed, EMBASE, and the Cochrane Database up to August 25, 2022. Meta-analyses were performed to evaluate the relationship between CCR and skeletal muscle [computed tomography-assessed skeletal muscle (CTASM), muscle strength, and physical performance], prognosis and important clinical outcomes in hospitalized adults. The pooled correlation coefficient, the area under the receiver operating characteristic (ROC) curves, and hazard ratio (HR) together with their 95% confidence intervals (CIs) were calculated. We also conducted subgroup analyses to explore the sources of heterogeneity.ResultsA total of 38 studies with 20,362 patients were eligible. These studies were of moderate to high quality. Our results showed that CCR was significant correlations with all CTASM types (Fisher’s Z ranged from 0.35 to 0.5; P values ranged from &lt; 0.01 to 0.01), handgrip strength (Fisher’s Z = 0.39; 95% CI, 0.32–0.45; P &lt; 0.001) and gait speed (Fisher’s Z = 0.25; 95% CI, 0.21–0.30; P &lt; 0.001). The ROC curves suggested that CCR had good diagnostic efficacy (0.689; 95% CI, 0.632–0.746; P &lt; 0.01) for sarcopenia. CCR can reliably predict mortality in hospitalized patients, which was confirmed by regression analysis of CCR as both continuous (HR 0.78; 95% CI, 0.72–0.84; P &lt; 0.01) and categorical variables (HR 2.05; 95% CI, 1.58–2.66; P &lt; 0.0001). In addition, less evidence showed that higher CCR was independently associated with a shorter duration of mechanical ventilation, reduced length of stay in the intensive care unit and hospital, less nutritional risk, and decreased complications in hospitalized patients.ConclusionCCR could be a simple, economical, and effective screening tool for sarcopenia in hospitalized patients, and it is a helpful prognostic factor for mortality and other important clinical outcomes.Systematic review registrationhttps://inplasy.com/inplasy-2022-9-0097/, identifier INPLASY202290097.
Retrospective cohort study to determine the effect of preinjury antiplatelet or anticoagulant therapy on mortality in patients with major trauma
Authors: Yamaji Fuminori , Okada Hideshi , Kamidani Ryo , Kawasaki Yuki , Yoshimura Genki , Mizuno Yosuke , Kitagawa Yuichiro , Fukuta Tetsuya , Ishihara Takuma , Suzuki Kodai , Miyake Takahito , Kanda Norihide , Doi Tomoaki , Yoshida Takahiro , Yoshida Shozo , Ogura Shinji 
Published: January 2023

ObjectiveThis study aimed to compare outcomes among patients who sustained major trauma from injury with and without receiving antiplatelet therapy (APT) or anticoagulant therapy (ACT) to test the hypothesis that APT does not increase the risk of mortality. However, ACT increases the mortality risk in the acute phase of trauma.MethodsPatients registered in the Japanese Observational body for Coagulation and Thrombolysis in Early Trauma 2 between April 2017 and March 2018 who had sustained a severe injury in any anatomic region of the body, as determined using an injury severity score (ISS) ≥ 16 were included in this retrospective cohort study. We analyzed the mortality within 24 h from the arrival using a multivariable linear regression analysis adjusted for several confounding variables.ResultsWe identified 1,186 eligible participants who met the inclusion criteria for this study: 105 in the APT (cases), 1,081 in the non-antiplatelet therapy (nAPT) group (controls), 65 in the ACT (cases), and 1,121 in the non-anticoagulant therapy (nACT) group (controls). The mortality within 24 h in the ACT group was significantly higher than in the nACT group (odds ratio 4.5; 95%CI: 1.2–16.79; p = 0.025); however, there was no significant difference between the two groups with or without the antiplatelet drug (odds ratio 0.32; 95%CI: 0.04–2.79; p = 0.3) administration. Other outcomes, like the 28-day mortality, mortality at discharge, and surgery for hemostasis, were not significantly different between regular users and non-users of either antiplatelet or anticoagulant drugs.ConclusionRegular antiplatelet medications did not increase mortality within 24 h, 28 days, or at discharge in patients with major trauma, suggesting that standard treatment, including surgery, is sufficient.