bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation of Bacillus amyloliquefaciens M13-RW0 from Soil and Evaluation
of its Antifungal Activity
Authors: Naghdifar Shirin , Madani Mahboobeh , Shakib Pegah 
Published: May 2023

Background:
The frequency of observed invasive Aspergillosis has increased in recent
years. Infection with other molds happens but does not lead to a large proportion of invasive infections.
The present study aims to isolate Bacillus amyloliquefaciens M13-RW0 from soil and evaluate
its antifungal effects against some saprophytic fungi, such as Aspergillus niger, Aspergillus flavus, and
Mucor hiemalis.

Materials and Methods:
In this research, a total of 150 samples (from the soil, air, and surfaces) were
prepared from different parts of Isfahan, Iran. Isolation and purification of growing bacteria were conducted
using the nutrient agar medium. The inhibitory effects of 100 isolated bacteria were evaluated
against the growth of A. niger, A. flavus, and M. hiemalis, 4 bacteria were isolated with inhibitory effects
against the selected fungi, and consequently, one of the bacteria isolated from the soil samples
was found to show the highest inhibition of fungal growth. Quantitative evaluation of the growth inhibitory
effect was performed using linear culturing of fungal suspension (104 spore/ml) at distances
of 5, 10, 15, 20, 25, and 30mm from bacterial isolate (0.5 McFarland) on Sabouraud Dextrose Agar
(SDA) medium. Results were checked 24, 48, 72, and 96 hours later. The bacterial isolate with the
most inhibitory effect was identified by phenotypic and molecular tests.

Results:
According to the results, among the 4 inhibitory bacterial isolates, Bacillus amyloliquefaciens
strain M13-RW01, isolated from the soil samples, was identified as the bacterium with the most significant
potential antifungal activity. The strong inhibitory effect was revealed after 48 hours for all
distances of 15mm and more between the fungi and the bacterium.

Conclusion:
The identified bacterium could not only be considered an inhibitor bacterium against saprophytic
fungi but could also be put forward to help produce new antifungal drugs for controlling fungal
diseases.
A Scope of Chiral Cyclopropanations of Various α-functionalized Diazoketone
Derivatives with Styrene Catalyzed by p-Nitro Ru(II)-diphenyl-
Pheox Complex
Authors: Chi Le Thi Loan , Iwasa Seiji 
Published: May 2023

Abstract:
Following the success of the first intermolecular cyclopropanations of diazo acetoxy acetone
with olefins using a novel p-nitro-Ru(II)-diphenyl-Pheox catalyst, other α-functionalized diazoketones
have synthesized using the same reaction conditions. Consequently, chiral cyclopropyl products
were formed with excellent yield (up to 87% yield), high diastereoselectivity (up to 99:1), and
high enantioselectivity (up to 90% ee).
Discovery of Natural Compounds as SARS-CoV-2’s Main Protease Inhibitors by Docking-based Virtual Screening
Authors: Wang Jing , Jiang Yu , Wu Yingnan , Ma Yuheng , Yu Hui , Wang Zhanli 
Published: May 2023

Introduction:
The novel coronavirus disease (COVID-19) is a viral disease caused by severe
acute respiratory syndrome coronavirus 2 (SAR-CoV-2). The development of antiviral drugs has enhaced
treatment of COVID-19. SARS-CoV-2 main protease (Mpro) is a key enzyme responsible for viral replication
and transcription. This study aimed to identify new natural structures for the design of SARS-CoV-2
Mpro inhibitors.

Methods:
In this present work, The CDOCKER protocol and scoring functions were validated. The validated
docking-based virtual screening approach was then employed to search the in-house database of
natural compounds for potential lead compounds as SARS-CoV-2 Mpro inhibitors. The top 3 compounds
were further biologically evaluated in vitro.

Results and Discussion:
Docking studies of the known ligand GC-376 led to results consistent with cocrystallized
data (PDB ID: 7D1M). Additionally, the effectiveness of docking scoring functions was validated
by using the training set consisting of 15 active compounds and 15 inactive compounds. Then, the
in-house database of natural compounds (overall 34,439 natural compounds) was subjected to dockingbased
virtual screening resulting in the identification of the top 100 compounds having relatively better
docking scores. Among them, the highest ranking 3 compounds (W-1, W-2, and W-3) were biologically
evaluated in vitro for their inhibitory activity against SARS-CoV-2 Mpro, and compound W-1 was identified
as the most potent SARS-CoV-2 Mpro inhibitor with an IC50 value of 63 ± 3 μM. Interestingly, it appeared
that the in vitro activities of compounds W-1, W-2, and W-3 were in agreement with their molecular
modeling data.

Conclusion:
Our results provided a useful reference for the discovery of novel natural SARS-CoV-2 Mpro
inhibitors by virtual screening.
Quercetin: A Comprehensive Review
Authors: Kumar Dharmendra , Sharma Pramod Kumar 
Published: May 2023

Abstract:
Quercetin is a plant pigment found in many fruits, vegetables, beverages, and other parts
of plants, such as leaves, flowers, bark, stems, and roots. The rich sources of quercetin are the dock,
watercress, sweet potato, onion, grapes, berries, cherries, and broccoli. Quercetin exhibits various
pharmacological activities, such as anticancer, antiviral, anti-inflammatory, and antioxidant. Several
studies have reported quercetin as a potential anticancer compound. This review article provides
information on the role of quercetin in many types of cancer, such as breast cancer, colon cancer,
liver cancer, lung cancer, prostate cancer, bladder cancer, gastric cancer, bone cancer, blood cancer,
brain cancer, cervical cancer, head and neck cancer, skin cancer, eye cancer, thyroid cancer, ovarian
cancer, kidney cancer, and mesothelioma cancer. The present review emphasizes the anticancer
activity of quercetin via different mechanisms, such as induced apoptosis, inhibition of tumor progression,
cancer cell cycle arrest via different pathways, decreased proliferation, modification of the
tumor microenvironment, etc.
Redefining the Roles of Aspirin across the Spectrum of Cardiovascular
Disease Prevention
Authors: Brown Matthew T. , Bortfeld Kristina S. , Sperling Laurence S. , Wenger Nanette K. 
Published: May 2023

Abstract:
Even before its role in platelet inhibition was fully characterized in the 1980s, aspirin
had been incorporated into the cardiovascular disease care algorithm. Early trials examining its use in
unstable angina and acute myocardial infarction revealed evidence of its protective role in the secondary
prevention of atherosclerotic cardiovascular disease (ASCVD). Large trials assessing use in the
primary prevention setting and optimal dosing regimens were studied in the late 1990s and early
2000s. As a cornerstone of cardiovascular care, aspirin was incorporated into primary and secondary
ASCVD prevention guidelines in the United States and mechanical heart valve guidelines. However,
in recent years, with significant advances in medical and interventional ASCVD therapies, scrutiny
has been placed on the bleeding profile of aspirin, and guidelines have adapted to new evidence. Updates
in primary prevention guidelines reserve aspirin only for patients at higher ASCVD risk and low
bleeding risk - though questions remain in ASCVD risk assessment as risk-enhancing factors have
proven difficult to incorporate on a population level. New thoughts regarding aspirin use in secondary
prevention - especially with the concomitant use of anticoagulants - have altered recommendations as
additional data accrued. Finally, a recommendation for aspirin and vitamin K antagonists with mechanical
heart valves has been modified. Despite aspirin losing a foothold in cardiovascular care, new
evidence has strengthened claims for its use in women at high risk for preeclampsia.
Potential Synergistic Effect of F.carica Against P. aeruginosa Intended for
the Management of Nosocomial Infection
Authors: Zaidi Kamal Uddin , Shah Fatema , Khan Firoz Naem 
Published: May 2023

Background:
Multi-drug resistance of Pseudomonas aeruginosa comprises the most vital
obstacles in the antibacterial fight worldwide. The innovation of novel and effective antibacterial
and/or resistance modulators is crucial to variance the extent of resistance or to reverse multidrug resistance.

Objective:
This study aimed to determine the in vitro antibacterial activity of the F.carica against the
Pseudomonas aeruginosa associated with nosocomial infection.

Methods:
We studied different extracts of F.carica in methanol, benzene and water with antibiotics
and their synergistic effect against P. aeruginosa using minimum inhibitory concentration and fraction
inhibitory concentration index.

Results:
Our results revealed that the methanol extract of leaves possessed maximum phyto
constituents and were active against P. aeruginosa. Methanol extracts of leaves alone and in combination with antibiotics showed a higher zone of inhibition and were synergistic <0.5) compared to other
extracts. Moreover, the combination of ofloxacin with all solvent extracts enhanced the synergistic antibacterial activity with respect to other antibiotics used.

Conclusion:
We explored the synergistic effects of F. carica methanol extracts alone and with antibiotics was the most potential against P. aeruginosa.
Formulation, Optimization, and In vitro Characterization of Cilnidipineloaded
Self-emulsifying Drug Delivery System
Authors: Kadian Renu , Nanda Arun 
Published: May 2023

Aim:
The goal of this research was to formulate and optimize a cost-effective self-emulsifying drug delivery system (SEDDS) of cilnidipine to increase its dissolution rate. Cilni-dipine is a BCS class II active pharmaceutical ingredient, which limits its use.

Methods:
Cilnidipine's solubility in various oils, surfactants, and cosurfactants, has been investigat-ed. To determine if there is any interaction between cilnidipine and certain excipients, drug compat-ibility tests were carried out. Based on phase solubility and compatibility studies, two combinations (Canola oil, Tween 80, and PEG 300; Peanut oil, Cremophor EL, and PEG 200) were prepared to create ternary phase diagrams for selecting the best combination with higher microemulsion region and to identify the range of concentration of excipients. Cilnidipine-loaded-SEDDS formulation was prepared by incorporating Canola oil, Tween 80, and PEG 300. For achieving the best formula-tion, D-optimal mixture design was used. The optimized SEDDS formulation was evaluated for globule size, zeta potential, drug release, drug content, self-emulsification time, and stability stud-ies.

Results:
The zeta potential (Y1) and globule size (Y2) of the optimized SEDDS formulation were found to be -36mV and 124.3nm, respectively. The optimized SEDDS formulation showed more than 98% drug release within 15 min in 10% ethanolic 0.1N HCl media, which was significantly higher than that of the pure drug (7.5%) and marketed tablet (~21%). The optimized formulation's self-emulsification time, drug content, and cloud point were 55s, 99.97 ± 1.57 %, and 75.6℃, re-spectively. After stability studies, there was no evidence of phase separation, colour change, and change in globule size.

Conclusion::
A significant improvement in in vitro drug release was observed from cilnidipine-loaded-SEDDS.
Futuristic Approach to Cholesterol Detection by Utilizing Non-invasive
Techniques
Authors: Sadasivuni Kishor Kumar , Geetha Mithra , Al-Maadeed Somaya , G.A. Muthalif Asan , M.S Sajna , Shabil Sha Mizaj 
Published: May 2023

Abstract:
Regular blood cholesterol control is an integral part of healthcare for detecting cardio-vascular issues immediately. Existing procedures are mostly intrusive and necessitate the collection of blood samples. Furthermore, because of the danger of infection, bruising, and/or haematoma, this measurement method may not be appropriate for continuous or regular examinations. As a result, an alternate option is required, which is known as the noninvasive (NI) approach that does not necessi-tate the collection of blood samples. Because NI approaches give painless and precise answers, they can be used in place of intrusive procedures. This review article includes a comprehensive investi-gation on NI methodologies and various NI approaches for detecting cholesterol in the bloodstream. It is important to note that medical system possibilities are changing due to the algorithms for NI techniques, which ultimately project the need for patient monitoring via the internet of medical things (IoMT) and artificial intelligence (AI).
Hot Melt Extrusion: A Paradigm–Changing Technology
Authors: Bhamare Vaibhav G. , Kamble Ravindra K. 
Published: May 2023

Abstract:
Over the last few decades, hot melt extrusion (HME) has found extensive adaptability and
utility as a viable drug delivery option in the pharmaceutical industry. HME has already been validated
as a robust, novel technique mainly used for the correction of solubility and bioavailability of poorly
soluble drugs. In line with the scope of the current issue, this review appraises the value of HME as
a means of solubility enhancement of BCS class II drugs and presents an influential tool for the manufacturing
or production of drugs or chemicals. The drug development process can be shortened with
the use of hot melt extrusion technology, and the application of this process to analytical technology
can ease the manufacturing process. This review focuses on the tooling, utility, and manufacturing aspects
associated with hot melt extrusion technology.
An Extract-Transform-Load Process Design for the Incremental Loading of German Real-World Data Based on FHIR and OMOP CDM: Algorithm Development and Validation
Authors: Henke Elisa , Peng Yuan , Reinecke Ines , Zoch Michéle , Sedlmayr Martin , Bathelt Franziska 
Published: May 2023

Abstract

Background
In the Medical Informatics in Research and Care in University Medicine (MIRACUM) consortium, an IT-based clinical trial recruitment support system was developed based on the Observational Medical Outcomes Partnership (OMOP) Common Data Model (CDM). Currently, OMOP CDM is populated with German Fast Healthcare Interoperability Resources (FHIR) using an Extract-Transform-Load (ETL) process, which was designed as a bulk load. However, the computational effort that comes with an everyday full load is not efficient for daily recruitment.

Objective
The aim of this study is to extend our existing ETL process with the option of incremental loading to efficiently support daily updated data.

Methods
Based on our existing bulk ETL process, we performed an analysis to determine the requirements of incremental loading. Furthermore, a literature review was conducted to identify adaptable approaches. Based on this, we implemented three methods to integrate incremental loading into our ETL process. Lastly, a test suite was defined to evaluate the incremental loading for data correctness and performance compared to bulk loading.

Results
The resulting ETL process supports bulk and incremental loading. Performance tests show that the incremental load took 87.5% less execution time than the bulk load (2.12 min compared to 17.07 min) related to changes of 1 day, while no data differences occurred in OMOP CDM.

Conclusions
Since incremental loading is more efficient than a daily bulk load and both loading options result in the same amount of data, we recommend using bulk load for an initial load and switching to incremental load for daily updates. The resulting incremental ETL logic can be applied internationally since it is not restricted to German FHIR profiles.
Is Service with a Smile Enough to Satisfy Customers? Sincere and Insincere Smiles via Video and Vignette
Authors: Bakar Resekiani Mas , Ayu Fitriani , Nadjamuddin Lukman 
Published: May 2023

The current service trend does not merely involve face-to-face interactions, including smiling (high-contact-service) by service employees, but also using methods such as texting (low-contact service). Sincere and insincere smile studies have used a method involving dynamic (videos) and static stimuli (photographs). However, research into the different types of smiles is still limited to methods using a vignette. This study aims to examine the effect of the type of smile on customer satisfaction using a vignette and video. A total of 139 people participated in 2 (smile: sincere versus insincere) x 2 (media: vignette versus video) groups. The study found that there is an influence from the type of smile on customer satisfaction. A sincere smile using video produces greater customer satisfaction than using a vignette does, because the emotional contagion process through video is more real than it is through a vignette. The interesting finding from this study is that there is a gap in the insincere smile condition between using video and a vignette. The dissatisfaction of customers is greater when served with an insincere smile by text (vignette), compared with a face-to-face interaction by video. Video could only capture the muscle movement on a person’s cheeks (zygomaticus major), but it could not see the muscle wrinkles formed around the eyes (orbicularis oculi), compared to a vignette. This study implies that although the standard operational procedure (SOP) for service demands friendliness, a customer can feel whether a server’s smile is genuine or fake. The customer’s dissatisfaction toward an insincere smile will be higher with a low-contact service using text than a high-contact service with video.
La configuración del mercado global de la miel: un análisis de equivalencia estructural
Authors: Salazar Solano Vidal , Cabrera Pereyra José Antonio , Moreno Dena Jesús Mario , Rojas Rodríguez Isaac Shamir 
Published: May 2023

Objetivo: definir el rol de los países participantes en la estructura del comercio mundial de miel, a partir de las capacidades de acceso al mercado global como ofertantes, consumidores y/o reexportadores.
Diseño metodológico: el análisis está soportado por un diseño de investigación cuantitativo de corte transversal y alcance descriptivo. Se realizó un ejercicio de modelación por bloques para identificar grupos de comportamiento similar, en un contexto de relaciones de intercambio (centro-periferia), entre actores dominantes de la cadena y otros que gravitan alrededor del núcleo central. Se utilizaron datos binarios: 1 representa conexión o acceso al mercado y 0 la ausencia de intercambio.
Resultados: la modelación por bloques se confirma como herramienta analítica del rol de cada país competidor en un contexto de relaciones centro-periferia, en las que emergen posiciones centrales (poder de mercado, gobernabilidad), respecto al resto de agentes de la cadena. Como resultado de la modelación fue posible agrupar a los países en tres bloques: eje central, principales exportadores, mercados secundarios.
Hallazgos: la investigación aporta una síntesis de las estrategias de comercio global de miel: exportaciones desde los principales exportadores hacia mercados secundarios; exportaciones de principales exportadores hacia el eje central; exportaciones desde el eje central hacia mercados secundarios; exportaciones desde y hacia el eje central.
Limitaciones de la investigación: la binarización de datos representa pérdida de información, sin embargo, no impide analizar la configuración del mercado global de miel.
Manejo de segundos molares maxilares con dos raíces palatinas: reporte de dos casos
Authors: Lozano Gonzáles Oscar , Salas Orozco Marco Felipe , Castillo Silva Brenda Eréndira , Casillas Santana Miguel Ángel 
Published: May 2023

Introducción: el objetivo de estos reportes de casos es mostrar la importancia de tener siempre en mente las posibles variaciones anatómicas presentes en los órganos dentales que necesitan tratamiento del conducto radicular. A pesar de los estudios dedicados a informar la prevalencia de estas variaciones anatómicas, las variaciones en el número de raíces palatinas de los molares maxilares rara vez se tienen en cuenta debido a su incidencia extremadamente baja. Material y métodos: este reporte presenta dos casos clínicos en los que se hay molares maxilares con raíces palatales adicionales. El primer caso fue una mujer de 41 años, sin datos patológicos aparentes, que presentó una cavidad de acceso previa, pero sin signos radiográficos de patología periapical. El sondaje periodontal fue normal. El diagnóstico pulpar fue de terapia pulpar previamente iniciada y el diagnóstico periapical fue de tejidos periapicales sanos. Durante la inspección del piso de la cámara pulpar el conducto palatino se localizó más distalmente de lo normal, y eso hizo sospechar al operador de la presencia de un conducto radicular adicional. Se amplió ligeramente la cavidad de acceso en sentido mesiopalatino, lo cual dio como resultado la localización de un segundo conducto palatino en una raíz palatina extra. El segundo caso fue una mujer de 28 años de edad con antecedentes médicos no contributivos, se presentó con dolor a los cambios térmicos en el segundo molar maxilar derecho. El diagnóstico pulpar fue pulpitis irreversible sintomática y el diagnóstico periapical fue de periodontitis apical sintomática. La cavidad de acceso tenía un diseño cuadrangular y se ubicaron cuatro conductos radiculares, de los cuales un conducto correspondía a un segundo conducto radicular palatino en su propia raíz palatina. Después de la localización de las raíces palatinas extras en estos dos casos, ambos pudieron ser negociados, instrumentados y obturados. Resultados: durante el tratamiento del conducto radicular se identificaron los tipos I y II del sistema de clasificación de raíces palatinas propuesto por Chritie y colaboradores para los molares maxilares. Conclusiones: es importante tener en cuenta estas variaciones anatómicas en el número y la forma de las raíces palatinas de molares maxilares. Aunque su prevalencia puede considerarse extremadamente baja, en una práctica endodóntica diaria se pueden encontrar este tipo de casos complejos.