bluuebunny/crossref_metadata_2025_split huggingface dataset:
Group meditation, addressing stigma, and “mental health days”: Recommendations for integrating self‐awareness practices into genetic counseling graduate programs
Authors: Bulmer Laura , Stanley Christy , Knauth Lauren Loffredo , Doyle Lauren E. , Mills Rachel 
Published: August 2023

AbstractGenetic counseling graduate programs provide a rigorous curriculum comprised of coursework encompassing counseling and medical genetics, fieldwork, and research experience. Students face similar emotional and mental demands as practicing genetic counselors while also experiencing stressors commonly associated with graduate study. Increased self‐awareness may help combat these stressors. This mixed‐methods study surveyed 154 genetic counseling graduate students to determine the types of self‐awareness practices they would like to have included in their graduate training and surveyed 11 program faculty regarding the feasibility of implementing these practices. The students' most preferred practices were self‐reflection (n = 73, 47.4%), support from peers, colleagues, and/or supervisors (n = 71, 46.1%), and mental health counseling (n = 71, 46.1%). Analysis of responses to open‐ended questions capturing students' recommendations for programs yielded six recurrent themes: (1) Consistent, Structured Practice with Accountability, (2) Emphasis on Mental Health, (3) Practical Techniques, (4) Access to Resources, (5) Encouragement and Support, and (6) Barriers to Implementation. Many students suggested that programs should incorporate repetitive exercises that could be implemented on a schedule with an emphasis on consistency (Theme 1). Students also emphasized the importance of providing exposure to multiple examples of self‐awareness practices, so they could find an approach that was most beneficial on an individual basis (Theme 3). These findings were shared with program faculty via a presentation at the Association for Genetic Counseling Program Directors annual meeting, and attendees were subsequently surveyed regarding self‐awareness practices currently integrated into their curriculum, as well as the feasibility and likelihood of integrating new practices. Program faculty respondents indicated that most of the recommended practices were included in their curriculum already or would be feasible and likely to incorporate. These results provide insight into the attitudes of genetic counseling students toward structured practice in self‐awareness and how genetic counseling graduate programs might integrate such practices into the curriculum.
A novel model incorporating chromatin regulatory factors for risk stratification, prognosis prediction, and characterization of the microenvironment in Wilms tumor
Authors: Zhao Xiaodong , Chu Xiaobin , Song Lei , Tang Weichun 
Published: August 2023

AbstractBackgroundWilms tumor, also known as nephroblastoma, a pediatric most‐frequent malignant‐kidney tumor, may be regulated and influenced by transcriptional and epigenetic mechanisms. Chromatin regulatory factors (CRs) play key roles in epigenetic regulation. The present study aimed to explore the involvement of CRs in the development of nephroblastoma.MethodsRNA‐sequencing and clinical information of nephroblastoma samples were obtained by downloading data from the TARGET database. The Limma package was utilized to perform differential expression analysis of genes (DEGs) between the tumor group and the control group. A Venn map was used for intersection of differential genes and CRs and to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of DEGs using the clusterProfiler package. LASSO and Cox analyses were used to construct CR‐related risk models and were evaluated based on clinical parameters. A receiver operating characteristic curve was employed to assess the diagnostic performance of risk model. Furthermore, we used a single‐sample gene set enrichment analysis algorithm for immune cell infiltration analysis. Finally, to confirm the transcriptome expression of pivotal genes in human nephroblastoma cell lines, a quantitative real‐time PCR was employed.ResultsFifteen key CRs were obtained through analysis in nephroblastoma and then the risk model based on 13 important CRs was constructed using the transcriptome data of nephroblastoma. Using the risk model, pediatric nephroblastoma patients were stratified into high‐ and low‐risk groups based on their individual risk scores. The risk score of CRs can predict adverse outcomes in pediatric nephroblastoma, and this gene cluster is closely related to various immunity characteristics of nephroblastoma. Moreover, the nephroblastoma cell line exhibited higher expression levels of prognostic genes (VRK1, ARNTL, RIT1, PRDM6, and TSPY1) compared to the HEK293 T cell line.ConclusionsThe risk characteristics derived from CRs have tremendous significance in predicting prognosis and guiding clinical classification and intervention strategies for pediatric nephroblastoma.
Differentiation Between the Low and High Trans‐Stenotic Pressure Gradient in Patients With Idiopathic Intracranial Hypertension Using 4D Flow MRI‐Derived Hemodynamic Parameters
Authors: Bi Jingfeng , Li Zhiye , Zhang Xue , Bai Xiaoyan , Zhao Xingquan , Qu Hui , Kong Qingle , An Jing , Mo Dapeng , Sui Binbin 
Published: August 2023

BackgroundTrans‐stenotic pressure gradient (TPG) measurement is essential for idiopathic intracranial hypertension (IIH) patients with transverse sinus (TS) stenosis. Four‐D flow MRI may provide a noninvasive imaging method for differentiation of IIH patients with different TPG.PurposeTo investigate the associations between 4D flow parameters and TPG, and to evaluate the diagnostic performance of 4D flow parameters in differentiating patients with high TPG (GroupHP) from low TPG (GroupLP).Study typeProspective.Population31 IIH patients with TS stenosis (age, 38 ± 12 years; 23 females) and 5 healthy volunteers (age, 25 ± 1 years; 2 females).Field Strength/Sequence3T, 3D phase contrast MR venography, and gradient recalled echo 4D flow sequences.AssessmentScan‐rescan reproducibility of 4D flow parameters were performed. The correlation between TPG and flow parameters was analyzed. The netflow and velocity difference between inflow plane, outflow plane, and the stenosis plane were calculated and compared between GroupHP and GroupLP.Statistical TestsPearson's correlation or Spearman's rank correlation coefficient, Independent samples t‐test or Wilcoxon rank‐sum test, Intra‐class correlation coefficient (ICC), Bland–Altman analyses, Receiver operating characteristic curves. A P value <0.05 was considered significant.ResultsSignificant correlations were found between TPG and netflow parameters including Favg,out‐s, Favg,in‐s, Fmax,out‐s, and Fmax,in‐s (r = 0.525–0.565). Significant differences were found in Favg,out‐s, Fmax,out‐s, Favg,in‐s, and Fmax,in‐s between GroupHP and GroupLP. Using the cut‐off value of 2.19 mL/sec, the Favg,out‐s showed good estimate performance in distinguishing GroupHP from GroupLP (AUC = 0.856). The ICC (ranged 0.905–0.948) and Bland–Altman plots indicated good scan‐rescan reproducibility.Data Conclusions4D flow MRI derived flow parameters showed good correlations with TPG in IIH patients with TS stenosis. Netflow difference between outflow and stenosis location at TS shows the good performance in differentiating GroupHP and GroupLP cases.Level of Evidence2Technical EfficacyStage 2
A novel humanized tri‐receptor transgenic mouse model of HAdV infection and pathogenesis
Authors: Wang Yawei , Wang Min , Bao Renlong , Wang Ligui , Du Xinying , Qiu Shaofu , Yang Chaojie , Song Hongbin 
Published: August 2023

AbstractHuman adenovirus (HAdV) is a highly virulent respiratory pathogen that poses clinical challenges in terms of diagnostics and treatment. Currently, no effective therapeutic drugs or prophylactic vaccines are available for HAdV infections. One factor contributing to this deficiency is that existing animal models, including wild‐type and single‐receptor transgenic mice, are unsuitable for HAdV proliferation and pathology testing. In this study, a tri‐receptor transgenic mouse model expressing the three best‐characterized human cellular receptors for HAdV (hCAR, hCD46, and hDSG2) was generated and validated via analysis of transgene insertion, receptor mRNA expression, and protein abundance distribution. Following HAdV‐7 infection, the tri‐receptor mice exhibited high transcription levels at the early and late stages of the HAdV gene, as well as viral protein expression. Furthermore, the tri‐receptor mice infected with HAdV exhibited dysregulated cytokine responses and multiple tissue lesions. This transgenic mouse model represents human HAdV infection and pathogenesis with more accuracy than any other reported animal model. As such, this model facilitates the comprehensive investigation of HAdV pathogenesis as well as the evaluation of potential vaccines and therapeutic modalities for HAdV.
Reliability of gridded temperature datasets to monitor surface air temperature variability over Bolivia
Authors: Satgé F. , Pillco R. , Molina‐Carpio J. , Mollinedo P. Pacheco , Bonnet M‐P. 
Published: August 2023

AbstractSix gridded temperature datasets (T‐datasets) were evaluated for the first time over the South American continent, through the case study of Bolivia, by comparing them with temperature records acquired from 82 meteorological stations spanning the 1995–2010 period. The comparisons were carried out at the daily time step considering different seasons (annual scale, austral summer and austral winter) and regions (Amazon, La Plata and Altiplano basins). Overall, the climate hazards group infrared temperature with stations (CHIRTS) and the climate prediction centre (CPC) T‐datasets provided the most reliable mean daily temperature (Tmean) and also described well the temporal variability of minimum and maximum daily temperature estimates (Tn and Tx). Tmean, Tn and Tx trends were analysed over the 1983–2016 period to observe temperature temporal evolution across the three regions. Despite some general agreements between the trends (Tmean, Tx and Tn), large discrepancies are also observed. It was found that CPC overestimates and CHIRTS underestimates mean temperature trends and that CPC (CHIRTS) was better than CHIRTS (CPC) to estimate Tx (Tn) trends, both in magnitude and space. Furthermore, opposing trends (i.e., warming and cooling) are described by CPC and CHIRTS for some specific regions, which call into question their reliability for such analyses. These findings highlight the need to validate gridded temperature products with reliable ground data for the regions under study, particularly if they have a wide elevation range.
Regionalization of rainfall in the upper Madeira basin based on interannual and decadal variability: A multi‐seasonal approach
Authors: Molina‐Carpio Jorge , Rivera Irma Ayes , Espinoza‐Romero Daniel , Cerón Wilmar Loaiza , Espinoza Jhan‐Carlo , Ronchail Josyane 
Published: August 2023

AbstractIdentifying rainfall regions associated with specific modes of variability is of practical interest for water resources management, seasonal forecasting, and mitigation of weather‐related risks. This study aims to identify homogeneous rainfall regions within the ~1 million km2 Upper Madeira River basin—southwestern Amazon—by their interannual and decadal variability and relates this variability to ocean indices. An observed dataset of 146 ground‐based rainfall stations, distributed throughout the Andes and the Amazon, and homogenized at the monthly time‐step for the period 1980–2016, was used for the analysis. With no spatial constraints, hierarchical cluster analysis and principal component analysis (PCA) optimally grouped stations into 10 rainfall homogenous regions. The value of the regionalization for interpreting the rainfall variability was evaluated by relating the seasonal rainfall time series of the regions with ocean indices. Then, by applying PCA to seasonal rainfall series and linking the principal components to sea surface temperature and ocean indices, an insight into the main large‐scale drivers of the rainfall spatio‐temporal variability in this basin at interannual and decadal scales is provided. This analysis identified differences in the year‐round influences of the tropical Pacific and/or Atlantic oceans on the 10 homogenous regions.
Curiosity Causes Creativity? Revealing the Reinforcement Circle between State Curiosity and Creativity
Authors: Ma Jie (Yonas) , Wei Wenyuan 
Published: August 2023

ABSTRACTCuriosity has long been extolled as a seed for employee creativity. This causality is plausible when considering curiosity as a stable trait. However, curiosity can also oscillate as a transitory state, thus complicating the causal sequence between such state curiosity and creativity. To clarify the causal ordering and achieve a refined understanding of what role state curiosity plays in the creative process, we utilize a random‐intercept cross‐lagged panel model (RI‐CLPM) to estimate the reciprocal effects between state curiosity and creativity at the within‐person level. With a full longitudinal panel data across three‐time points (N = 400), we find that at the within‐person level, state curiosity has a positive lagged effect on creativity and creativity also shows a positive lagged effect on state curiosity. This research highlights that curiosity and creativity, in fact, mutually reinforce each other.
Dioscin ameliorates doxorubicin‐induced heart failure via inhibiting autophagy and apoptosis by controlling the PDK1‐mediated Akt/mTOR signaling pathway
Authors: Yuan Ling , Ji Hai‐Gang , Yan Xiao‐Jing , Liu Meng , Ding Yu‐Han , Chen Xiao‐Hu 
Published: August 2023

AbstractHeart failure (HF) is a disease with high mortality and morbidity rate. Autophagy is critically implicated in HF progression. The current research was designed to investigate the function of Dioscin on oxidative stress, autophagy, and apoptosis in HF. In this study, doxorubicin (Dox) was employed to induce HF model and HL‐1 cell damage model. Echocardiography implied that Dioscin could dramatically relieve heart function in vivo. Western blotting determined that Dioscin treatment reversed the promotive effect of autophagy caused by Dox through modulating levels of key autophagy‐associated molecules, including Atg5 and Beclin1. Dioscin also impaired apoptosis by regulating apoptosis‐related protein, including Bcl‐2 and cleaved caspase‐3 following Dox treatment in vivo and in vitro. Furthermore, the impacts of Dioscin were mediated by upregulation of PDK1‐mediated Akt/mTOR signaling. The mTOR inhibitor (rapamycin) could counteract the therapeutic impact of Dioscin in vitro. Taken together, Dioscin could relieve cardiac function through blocking apoptosis and autophagy by activating the PDK1‐elicited Akt/mTOR pathway.
Antibiotic Sensitivity and Nasal Microbiome in Patients with Acute Bacterial Rhinosinusitis
Authors: Gwak Ho‐Jin , Lee Hyeon A. , Jeong Jae Yeong , Lee Yangsoon , Rho Mina , Cho Seok Hyun 
Published: August 2023

ObjectivesAcute rhinosinusitis (ARS) is a common upper respiratory tract infection that is mostly of viral origin. However, little is known about the nasal microbiome profile at presentation and the changes caused by antibiotics in acute bacterial rhinosinusitis (ABRS).MethodsThis was a prospective single‐center study. Overall, 43 ARS patients were screened and were assessed with the symptom questionnaires, nasal endoscopy, and Water's view. Five healthy subjects were recruited as controls. Middle meatal mucus samples were obtained using a cotton swab (for bacterial culture and antimicrobial susceptibility testing) and the suction technique (for 16S rRNA sequencing). After 1 week of antibiotic use (amoxicillin with clavulanic acid), we enrolled 13 patients with ABRS with positive isolates and middle meatal samples for 16S rRNA sequencing were obtained again.ResultsOverall, we demonstrated a significantly lower abundance of the Lactobacillaceae family in ABRS patients than in healthy controls. Resistant ABRS had different characteristics of middle meatal microbiomes when compared to sensitive ABRS as follows: (1) lower proportion of lactic acid bacteria, (2) increased pathogens such as Rhodococcus sp., Massila sp., Acinetobacter sp., and H. influenza, and (3) increased beta diversity. However, no remarkable changes were observed in the middle meatal microbiome after antibiotic use.ConclusionWe showed the roles of Lactobacillaceae in ABRS, and Acinetobacter and Massilia in case of amoxicillin resistance.Level of Evidence3 Laryngoscope, 134:1081–1088, 2024
A Consistent Endoscopic Landmark to Identify the Anterior Ethmoidal Artery
Authors: Mundy David C. , Yan Carol H. , Tyler Matthew A. , Patel Zara M. 
Published: August 2023

ObjectiveThe anterior ethmoidal artery (AEA) is an important structure to identify during endoscopic sinus surgery. Although identification on imaging is easily taught, a consistent endoscopic landmark for the AEA, independent of anatomic ethmoid cell variation, is lacking, leaving many surgeons unclear about the exact location without dependence on navigation. Here, we describe a consistent endoscopic landmark, regardless of anatomical ethmoid variation.MethodsWe prospectively enrolled adult patients undergoing endoscopic surgery involving frontal and ethmoid sinuses in this observational study. The AEA landmark was defined simply as the septation or ridge one step back along the ethmoid skull base from the posterior table of the frontal sinus. The gold standard to calculate the sensitivity of our endoscopic landmark was an image‐navigation system, registered to within 1.5 mm accuracy, locating the AEA within three planes. Both endoscopic and computerized tomography (CT) images of the pointer at the landmark were taken simultaneously. The concordance of endoscopic to navigation images was independently assessed by three blinded rhinologists.ResultsForty patients were included in our study with 73 sides analyzed. Diagnoses included chronic rhinosinusitis without polyps (52.5%), with polyps (22.5%), recurrent acute sinusitis (15%), sinonasal tumors (7.5%), and odontogenic sinusitis (2.5%). The AEA was accurately identified using our endoscopic landmark in 97.3% of the cases (71/73). Of the two cases in which the AEA was not found within the landmark, the artery was located ≤1 mm posteriorly.ConclusionWe describe a consistent endoscopic landmark to identify the AEA, conserved across various clinical diagnoses and anatomic variations in sinus structure.Level of Evidence3 Laryngoscope, 134:1096–1099, 2024
Use of Perioperative Virtual Reality Experiences on Anxiety and Pain: A Randomized Comparative Trial
Authors: Pandrangi Vivek C , Low Garren , Slijepcevic Allison , Shah Suparna , Shindo Maisie , Schindler Joshua , Colaianni Alessa , Clayburgh Daniel , Andersen Peter , Flint Paul , Wax Mark K , Li Ryan J 
Published: August 2023

ObjectiveTo evaluate the effect of different virtual reality (VR) experiences on perioperative anxiety and pain among patients undergoing head and neck surgery.MethodsProspective, randomized, comparative trial among patients undergoing outpatient head and neck surgery from December 2021 to April 2022 at a single academic institution. Group 1 utilized a preoperative VR gaming experience and a postoperative VR mindfulness experience, while Group 2 utilized the same interventions in the reverse order. Anxiety and pain were measured using visual analog scales (VAS). Primary outcomes were changes in post‐intervention anxiety and pain. Secondary outcomes included vital sign changes and subjective patient experiences using a 5‐point Likert scale.ResultsThere were 32 patients randomized for inclusion, with 16 patients per group. The majority of patients were female (65.6%) and mean (standard deviation) age was 47.3 (16.7) years. After outlier exclusion, there were no differences in post‐intervention preoperative anxiety reduction (Group 1 vs. Group 2, median [IQR]: −12.0 [15] vs. −10.5 [13], p = 0.62). There were minor differences in vital sign changes (p < 0.05). Among the 10 patients in Group 1 and 12 patients in Group 2 who completed postoperative VR use, there were no differences in post‐intervention pain reduction (−8.5 [22.3] vs. −7.5 [19.3], p = 0.95) or vital sign changes (p > 0.05). There were no differences in questionnaire responses, with high satisfaction in both groups (p > 0.05). No adverse events encountered.ConclusionsUse of different VR experiences among patients undergoing outpatient head and neck surgery appears associated with similar reductions in perioperative anxiety and pain.Level of Evidence2 Laryngoscope, 134:1197–1202, 2024
Bell's palsy in pregnancy: A scoping review of risk factors, treatment and outcomes
Authors: Jones Holly , Hintze Justin , Slattery Fionn , Gendre Adrien 
Published: August 2023

AbstractObjectiveThere are limited studies reporting on Bell's palsy and pregnancy. Our study aimed to evaluate risk factors, current treatment options and facial function outcomes in women who developed Bell's palsy in pregnancy. To our knowledge this is the first review analyzing these factors.Data sources/review methodsA search of PubMed/MEDLINE, Embase, Web of Sciences and Scopus was carried out. Studies describing risk factors, treatment and/or facial function outcomes of Bell's palsy in pregnancy were included. PRISMA‐Scr guidelines were followed.ResultsThe search yielded 392 abstracts, of which 15 studies were included for analysis. It was not possible to perform a meta‐analysis due to small numbers and quality of studies. There were 559 patients included from the 15 studies. The third trimester was the most common time for Bell's palsy to occur (n = 364, 65%). Pre‐eclampsia was the most common co‐morbidity reported. The most common treatment was corticosteroids and the majority of patients had a complete recovery of their palsy (58%, n = 192).ConclusionThis analysis has evaluated all available data concerning risk factors, treatment and facial function outcomes of BP in pregnancy. The third trimester is the most common time for Bell's palsy to occur in pregnancy. There is currently a lack of high quality evidence into this condition in pregnancy.Level of evidence1.
Potential influence of microorganisms on the corrosion of carbon steel in the French high‐level long‐lived radioactive waste disposal context at 80°C
Authors: Diler Erwan , Leblanc Vanessa , Gueuné Hervé , Maillot Valérie , Linard Yannick , Charrier Gaëlle , Crusset Didier 
Published: August 2023

AbstractIn this study, experiments were carried out to assess the microbially influenced corrosion (MIC) risk in the context of the French high‐level radioactive waste disposal. The exposures were carried out at 80°C in different repository relevant conditions, including the presence of different cement‐grout mixtures as filling material. Biotic conditions with nutrient and nonsterile conditions with indigenous microbes added from Callovo Oxfordian clayey rock and without nutrients were considered. For biotic conditions, specific preparations of microbial inoculum were carried out from samples collected at Andra's Underground Research Laboratory and microorganisms from microbial culture collection centers. Corrosion kinetics were determined using traditional coupons and completed with real‐time corrosion sensors. Microbiological characterizations consisted of cultural approach, quantitative polymerase chain reaction, and next‐generation sequencing. The obtained results show no significant MIC and a reduced risk with the use of more alkaline filling material.