bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns in leaf traits of woody species and their environmental determinants in a humid karstic forest in southwest China
Authors: Long Qingzhi , Du Hu , Su Liang , Zeng Fuping , Lian Ziwen , Peng Wanxia , Tan Weining , Sun Rui , Wang Kelin 
Published: August 2023

IntroductionLeaf functional traits constitute a crucial component of plant functionality, providing insights into plants’ adaptability to the environment and their regulatory capacity in complex habitats. The response of leaf traits to environmental factors at the community level has garnered significant attention. Nevertheless, an examination of the environmental factors determining the spatial distribution of leaf traits in the karst region of southwest China remains absent.MethodsIn this study, we established a 25 ha plot within a karst forest and collected leaf samples from 144 woody species. We measured 14 leaf traits, including leaf area (LA), leaf thicknes (LT), specific leaf area (SLA), leaf length to width ratio (LW), leaf tissue density (LTD), leaf carbon concentration (LC), leaf nitrogen concentration (LN), and leaf phosphorus concentration (LP), leaf potassium concentration (LK), leaf calcium concentration (LCa), leaf magnesium Concentration (LMg), leaf carbon to nitrogen ratio (C/N), leaf carbon to phosphorus ratio (C/P), and leaf nitrogen to phosphorus ratio (N/P), to investigate the spatial distribution of community-level leaf traits and the response of the leaf trait community-weighted mean (CWM) to topographic, soil, and spatial factors.ResultsResults showed that the CWM of leaf traits display different spatial patterns, first, the highest CWM values for LT, LTD, C/N, and C/P at hilltops, second, the highest CWM values for LA, SLA, LW, LC, LN, LP, and LK at depressions, and third, the highest CWM values for LCa, LMg, and N/P at slopes. The correlation analysis showed that topographic factors were more correlated with leaf trait CWM than soil factors, with elevation and slope being the strongest correlations. RDA analysis showed that topographic factors explained higher percentage of leaf trait CWM than soil factors, with the highest percentage of 19.96% being explained by elevation among topographic factors. Variance Partitioning Analysis showed that the spatial distribution of leaf traits is predominantly influenced by the combined effects of topography and spatial factors (37%-47% explained), followed by purely spatial factors (24%-36% explained).DiscussionThe results could improve our understanding of community functional traits and their influencing factors in the karst region, which will contribute to a deeper understanding of the mechanisms that shape plant communities.
Maternal complications and associated factors among mothers who underwent a cesarean section at Gebretsadik Shewa general hospital: an institution based cross-sectional study
Authors: Negese Ketemaw , Belachew Dereje Zeleke 
Published: August 2023

IntroductionCesarean sections have played a major role in lowering maternal morbidity and mortality rates, but are a major concern in developing countries. This study aims to assess the magnitude of maternal complication and its associated factors among women who underwent a cesarean section at Gebretsadik Shewa general hospital, Southwest Ethiopia.MethodA hospital-based cross-sectional study was conducted in Gebretsadik Shewa general hospital. Data were extracted from 382 mothers' medical charts, retrieved from the labor and operations theatre log book registry using systematic random sampling technique. The extracted data was coded and entered into Epi Data version 3.0, and exported to Statistical Package for Social Sciences (SPSS) version 20 for analysis. Logistic regression analysis was conducted and significance and strength association was determined considering AOR with a 95% confidence level.ResultA total of 382 mothers' charts were reviewed; however, 368 charts were eligible for data entry. The age of the participants ranges between 16 and 42 years with mean and standard deviation of 26.1 ± 4.8 years. Maternal complication rate was 30.4% [95% CI: 25.8- 35.1]. Surgical site infection (10.3%), anemia (6.5%) and intraoperative bleeding (4.6%) were the most common. Multivariable logistic regression analysis showed that no antenatal care follow up, medical illness during pregnancy, emergency cesarean section and not receiving a prophylactic antibiotic were statistically associated with maternal complications.ConclusionThe incidence of maternal complication following cesarean delivery was unduly high. Community based education about antenatal care follow up and its importance should be further strengthened for favorable maternal and fetal outcomes.
Knowledge, attitudes, practices, and beliefs regarding prenatal alcohol consumption among women in Leyte, the Philippines
Authors: Huang Alice M. , Neale Matthew N. , Darveau Spencer C. , Sagliba Marianne J. , Moreno Amabelle J. , Urbina Maria Paz G. , Tallo Veronica , McDonald Emily A. , Jiz Mario A. , Friedman Jennifer F. 
Published: August 2023

ObjectivesFetal alcohol spectrum disorder (FASD) captures the broad range of emotional, cognitive, behavioral, and congenital abnormalities associated with maternal alcohol consumption, and women living in resource-limited settings may be higher risk. This study aims to examine knowledge, attitudes, practices, and beliefs (KAPB) of women in Leyte, The Philippines regarding prenatal alcohol consumption.MethodsOne hundred postpartum women were recruited from a birth cohort in Leyte. A prenatal alcohol use KAPB survey was constructed in Waray, the local language. The survey was administered in June-September 2019. Descriptive statistics, chi-squared test, and Fisher's exact test were used to analyze responses.ResultsSeventy-five percent of subjects reported drinking tuba, a local palm wine, during pregnancy. Most participants (75%) did not believe tuba contained alcohol. Women who believed tuba contains no alcohol were more likely to drink tuba during pregnancy (81.3%) than women who believed tuba contains alcohol (56.0%), X2(1, N = 100) = 6.41, p = .011. Women who drank tuba during pregnancy were more likely to believe tuba has health benefits (60%) than women who did not drink tuba during pregnancy (12%), Fisher's exact p &lt; .05, citing increased red blood cell count and unproven antiparasitic qualities. Fifteen percent of subjects reported having fed their babies tuba. Nearly all (98%) were willing to attenuate tuba/alcohol consumption if told that this practice negatively impacts pregnancies.ConclusionMisinformation about tuba appears widespread in Leyte. Educating women of reproductive age in Leyte regarding prenatal tuba use may lead to a reduction in tuba use.
Brain structural differences in children with fetal alcohol spectrum disorder and its subtypes
Authors: Boateng Theresah , Beauchamp Kathryn , Torres Faerl , Ruffaner-Hanson Chaselyn D. , Pinner John F. L. , Vakamudi Kishore , Cerros Cassandra , Hill Dina E. , Stephen Julia M. 
Published: August 2023

IntroductionThe teratogenic effects of prenatal alcohol exposure (PAE) have been examined in animal models and humans. The current study extends the prior literature by quantifying differences in brain structure for individuals with a fetal alcohol spectrum disorder (FASD) compared to typically developing controls, as well as examining FASD subtypes. We hypothesized the FASD group would reveal smaller brain volume, reduced cortical thickness, and reduced surface area compared to controls, with the partial fetal alcohol syndrome (pFAS)/fetal alcohol syndrome (FAS) subtypes showing the largest effects and the PAE/alcohol-related neurodevelopmental disorder (ARND) subtype revealing intermediate effects.MethodsThe sample consisted of 123 children and adolescents recruited from a single site including children with a diagnosis of FASD/PAE (26 males, 29 females) and controls (34 males, 34 females). Structural T1-weighted MRI scans were obtained on a 3T Trio TIM scanner and FreeSurfer v7.2 was used to quantify brain volume, cortical thickness, and surface area. Analyses examined effects by subgroup: pFAS/FAS (N= 32, Mage= 10.7 years, SEage= 0.79), PAE/ARND (N= 23, Mage= 10.8, SEage= 0.94), and controls (N= 68, Mage= 11.1, SEage= 0.54).ResultsTotal brain volume in children with an FASD was smaller relative to controls, but subtype analysis revealed only the pFAS/FAS group differed significantly from controls. Regional analyses similarly revealed reduced brain volume in frontal and temporal regions for children with pFAS/FAS, yet children diagnosed with PAE/ARND generally had similar volumes as controls. Notable differences to this pattern occurred in the cerebellum, caudate, and pallidum where children with pFAS/FAS and PAE/ARND revealed lower volume relative to controls. In the subset of participants who had neuropsychological testing, correlations between volume and IQ scores were observed. Goodness-of-Fit analysis by age revealed differences in developmental patterns (linear vs. quadratic) between groups in some cases.DiscussionThis study confirmed prior results indicating decreased brain volume in children with an FASD and extended the results by demonstrating differential effects by structure for FASD subtypes. It provides further evidence for a complex role of PAE in structural brain development that is likely related to the cognitive and behavioral effects experienced by children with an FASD.
Combining methylated SDC2 test in stool DNA, fecal immunochemical test, and tumor markers improves early detection of colorectal neoplasms
Authors: Zeng Tao , Huang Zhongchao , Yu Xufa , Zheng Li , Liu Tao , Tian Boyu , Xiao Siyu , Huang Jiahui 
Published: August 2023

ObjectiveTo explore the value of testing methylated SDC2 (SDC2) in stool DNA combined with fecal immunochemical test (FIT) and serum tumor markers (TM) for the early detection of colorectal neoplasms.MethodsA total of 533 patients, including 150 with CRC (67 with early-stage CRC), 23 with APL, 85 with non-advanced adenomas and general polyps, and 275 with benign lesions and healthy controls. SDC2 was detected by methylation-specific PCR, FIT (hemoglobin, Hb and transferrin, TF) was detected by immunoassay, and the relationships between SDC2, FIT, and clinicopathological features were analyzed. Pathological biopsy or colonoscopy were used as gold standards for diagnosis, and the diagnostic efficacy of SDC2 combined with FIT and TM in CRC and APL evaluated using receiver operating characteristic (ROC) curves.ResultsSDC2 positive rates in early-stage CRC and APL were 77.6% (38/49) and 41.2% (7/17), respectively, and combination of SDC2 with FIT increased the positive rates to 98.0% (48/49) and 82.4% (14/17). The positive rates of SDC2 combined with FIT assay in the APL and CRC groups at stages 0-IV were 82.4% (14/17), 85.7% (6/7), 100% (16/16), 100% (26/26), 97.4% (38/39), and 100% (22/22), respectively. Compared to the controls, both the CRC and APL groups showed significantly higher positive detection rates of fecal SDC2 and FIT (χ2 = 114.116, P &lt; 0.0001 and χ2 = 85.409, P &lt; 0.0001, respectively). Our results demonstrate a significant difference in the qualitative methods of SDC2 and FIT for the detection of colorectal neoplasms (McNemar test, P &lt; 0.0001). ROC curve analysis revealed that the sensitivities of SDC2 and FIT, alone or in combination, for the detection of early CRC and APL were 69.9%, 86.3%, and 93.9%, respectively (all P&lt;0.0001). When combined with CEA, the sensitivity increased to 97.3% (P&lt;0.0001).ConclusionsSDC2 facilitates colorectal neoplasms screening, and when combined with FIT, it enhances detection. Furthermore, the combination of SDC2 with FIT and CEA maximizes overall colorectal neoplasm detection.
Prognostic role of IL-8 in cancer patients treated with immune checkpoint inhibitors: a system review and meta-analysis
Authors: Zou Dan , Song Ailin , Yong Wei 
Published: August 2023

BackgroundImmune checkpoint inhibitors (ICIs) have been proven to be an effective treatment strategy for a variety of malignant tumors. However, only a subset of patients can benefit from ICIs due to factors such as drug resistance. Therefore, it is crucial to identify biomarkers that can accurately predict the efficacy of ICIs and provide a basis for individualized immunotherapy. In this study, we conducted a systematic review and meta-analysis to explore whether the chemokine interleukin 8 (IL-8) can be used as a biomarker to evaluate the efficacy of ICIs treatment.MethodsWe conducted a comprehensive search of several databases, including PubMed, Embase, Web of Science, and Cochrane, to identify relevant articles published up to June 08, 2023. Our inclusion criteria were limited to cohort studies and clinical trials that reported hazard ratios (HR) and 95% confidence intervals (CI) for overall survival (OS) and/or progression-free survival (PFS), as well as the objective response rate (ORR), in cancer patients with high and low IL-8 expression. For data analysis, we used Revman to generate forest plots, subgroup analysis, and assess publication bias. Additionally, Stata was utilized for sensitivity analysis and further examination of publication bias.ResultsA total of 24 datasets, involving 3190 participants, were selected from 14 studies. The meta-analysis revealed a reduction in ORR, OS, and/or PFS in the high IL-8 group after treatment with ICIs compared to the low IL-8 group.ConclusionIL-8 can serve as a biomarker for predicting the efficacy of ICIs. Patients with lower expression of IL-8 may benefit from ICIs treatment.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=383188, identifier CRD42022383188.
Economic evaluation of third-line neratinib plus capecitabine versus lapatinib plus capecitabine with HER2+ metastatic breast cancer
Authors: Ren Lanqi , Ren Ning , Zheng Yu , Yang Yibei , Xu Qiaoping 
Published: August 2023

BackgroundBreast cancer (BC) is one of the most common malignant tumors in women. In addition, human epidermal growth factor receptor 2-positive (HER2+) BC is overexpressed in 25% of BC patients, resulting in the predicament of poor prognosis. Although first- and second-line treatments have been established, optimum third-line treatment is still mired in controversies for HER2+ metastatic BC (mBC). Therefore, this study analyzes the cost-effectiveness of neratinib plus capecitabine (N+C) and lapatinib plus capecitabine (L+C) over a 5-year time horizon from a payer perspective.MethodsA half-cycle corrected four-state Markov model was established to simulate the course of BC events and deaths in N+C and L+C armed patients. The data of this model were derived from NCT01808573 trail and other published literatures. One-way deterministic sensitivity analysis (DSA) was conducted to investigate the impact of variables and probabilistic sensitivity analysis (PSA) was performed based on second-order Monte Carlo simulation. In addition, subgroup analysis was performed to verify its cost-effectiveness in China.ResultThe base-case results found that N+C was in dominant position in 82.70% of the generation scenarios, providing an improvement of 0.17 quality-adjusted life-years (QALYs) and a reduction of $1,861.28 compared with L+C. The ICER was $-1,3294.86/QALY, which did not exceed the willingness to pay (WTP) threshold, while in subgroup, the ICER decreased to $-2,448.17/QALY.ConclusionThis analysis indicated that the combination of neratinib plus capecitabine is likely to be cost-effective in comparison with lapatinib plus capecitabine in patients with HER2+ mBC who continues to progress during or after second-line HER2-targeted therapy. So neratinib plus capecitabine can become a third-line treatment option.
LM02 trial Perioperative treatment with panitumumab and FOLFIRI in patients with wild-type RAS, potentially resectable colorectal cancer liver metastases—a phase II study
Authors: Piringer Gudrun , Gruenberger Thomas , Thaler Josef , Kührer Irene , Kaczirek Klaus , Längle Friedrich , Viragos-Toth Istvan , Amann Arno , Eisterer Wolfgang , Függer Reinhold , Andel Johannes , Pichler Angelika , Stift Judith , Sölkner Lidija , Gnant Michael , Öfner Dietmar 
Published: August 2023

BackgroundTwenty percent of colorectal cancer liver metastases (CLMs) are initially resectable with a 5-year survival rate of 25%–40%. Perioperative folinic acid, 5-fluorouracil, oxaliplatin (FOLFOX) increases progression-free survival (PFS). In advanced disease, the addition of targeting therapies results in an overall survival (OS) advantage. The aim of this study was to evaluate panitumumab and FOLFIRI as perioperative therapy in resectable CLM.MethodsPatients with previously untreated, wild-type Rat sarcoma virus (RAS), and resectable CLM were included. Preoperative four and postoperative eight cycles of panitumumab and folinic acid, 5-fluorouracil, irinotecan (FOLFIRI) were administered. Primary objectives were efficacy and safety. Secondary endpoints included PFS and OS.ResultsWe enrolled 36 patients in seven centers in Austria (intention-to-treat analyses, 35 patients). There were 28 men and seven women, and the median age was 66 years. About 91.4% completed preoperative therapy and 82.9% underwent liver resection. The R0 resection rate was 82.7%. Twenty patients started and 12 patients completed postoperative chemotherapy. The objective radiological response rate after preoperative therapy was 65.7%. About 20% and 5.7% of patients had stable disease and progressive disease, respectively. The most common grade 3 adverse events were diarrhea, rash, and leukopenia during preoperative therapy. One patient died because of sepsis, and one had a pulmonary embolism grade 4. After surgery, two patients died because of hepatic failure. Most common grade 3 adverse events during postoperative therapy were skin toxicities/rash and leukopenia/neutropenia, and the two grade 4 adverse events were stroke and intestinal obstruction. Median PFS was 13.2 months. The OS rate at 12 and 24 months were 85.6% and 73.3%, respectively.ConclusionsPanitumumab and FOLFIRI as perioperative therapy for resectable CLM result in a radiological objective response rate in 65.7% of patients with a manageable grade 3 diarrhea rate of 14.3%. Median PFS was 13.2 months, and the 24-month OS rate was 73.3%. These data are insufficient to widen the indication of panitumumab from the unresectable setting to the setting of resectable CLM.
Effects of blood flow restriction training on bone metabolism: a systematic review and meta-analysis
Authors: Wang Xiaolin , Wang Yifei , Yang Xuezhen , Mohd Nasiruddin Nasnoor Juzaily Bin , Dong Delong , Samsudin Shamsulariffin Bin , Qin Xin-Min 
Published: August 2023

Introduction:The efficacy of low-intensity blood flow restriction (LI-BFR) training programs in bone metabolism remains unclear compared to low-intensity (LI) training and high-intensity (HI) training. The aim of this review was to quantitatively identify the effects of LI-BFR training on changes in bone formation markers (i.e., bone-specific alkaline phosphatase, BALP), bone resorption (i.e., C-terminal telopeptide of type I collagen, CTX) and bone mineral density (BMD) compared with conventional resistance training programmes. Additionally, the effectiveness of walking with and without BFR was assessed.Methods:PubMed, Scopus, SPORTDiscus, Web of Science and Google Scholar databases were searched for articles based on eligibility criteria. Review Manager Version 5.4 was used for Meta-analysis. Physiotherapy Evidence Database (PEDro) was applied to assess the methodological quality of studies.Results:12 articles were included in the meta-analysis, with a total of 378 participants. Meta-results showed that compared with LI training, LI-BFR training induced greater increments in BALP (young adults: MD = 6.70,p&lt; 0.001; old adults: MD = 3.94,p= 0.002), slight increments in BMD (young adults: MD = 0.05,p&lt; 0.00001; old adults: MD = 0.01,p&lt; 0.00001), and greater decrements in CTX (young adults: MD = −0.19,p= 0.15; old adults: MD = −0.07,p= 0.003). Compared with HI training, LI-BFR training produced smaller increments in BALP (young adults: MD = −6.87,p= 0.24; old adults: MD = −0.6,p= 0.58), similar increments in BMD (MD = −0.01,p= 0.76) and similar decrements in CTX (young adults: MD = 0,p= 0.96; old adults: MD = −0.08,p= 0.13). Although there were only two studies on walking training intervention, walking training with BFR had a better effect on bone metabolism than training without BFR.Discussion:In conclusion, LI-BFR training induces greater improvements in bone health than LI training, but is less effective than HI training. Therefore, LI-BFR training may be an effective and efficient way to improve bone health for untrained individuals, older adults, or those undergoing musculoskeletal rehabilitation.Clinical Trial Registration:[https://www.crd.york.ac.uk/prospero/], identifier [CRD42023411837].
Limited genetic changes observed during in situ and ex situ conservation in Nordic populations of red clover (Trifolium pratense)
Authors: Hagenblad Jenny , Aloisi Karolina , Marum Petter , Öhlund Linda , Solberg Svein Øivind , Asdal Åsmund , Palmé Anna 
Published: August 2023

IntroductionIn situ and ex situ conservation are the two main approaches for preserving genetic diversity. The advantages and disadvantages of the two approaches have been discussed but their genetic effects have not been fully evaluated.MethodsIn this study we investigate the effects of the two conservation approaches on genetic diversity in red clover. Seed samples collected from wild populations in Sweden and Norway in 1980, their subsequent generations created during seed regeneration at the gene bank and samples recollected from the same location as the original samples, were analyzed with microsatellite markers, alongside reference samples from cultivars.ResultsOverall, there was a differentiation between cultivars and the wild material and between wild material from Sweden and Norway. In general, the original collections clustered together with the later generations of the same accession in the gene bank, and with the recollected samples from the same location, and the level of diversity remained the same among samples of the same accession. Limited gene flow from cultivated varieties to the wild populations was detected; however, some wild individuals are likely to be escapees or affected by gene flow.DiscussionIn conclusion, there were examples of genetic changes within individual accessions both in situ and ex situ, as is also to be expected in any living population. However, we observed only limited genetic changes in both in situ and ex situ conservation over the generations included in this study and with the relatively large populations used in the ex situ conservation in the gene bank at NordGen.