bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative evaluation of the expression of enzymes of the ceramide <i>de novo</i> synthesis pathway in cardiac adipose tissue and blood vessels of cardiovascular patients
Authors: Gruzdeva O. V. , Dyleva Yu. A. , Belik E. V. , Uchasova E. G. , Ponasenko A. V. , Gorbatovskaya E. E. , Fanaskova E. A. , Ivanov S. V. , Stasev A. N. , Zinets M. G. , Barbarash O. L. 
Published: January 2023

Aim. To compare the expression of enzymes of the ceramide de novo synthesis pathway in cardiac adipose tissue (AT) and blood vessels of patients with coronary artery disease (CAD) and acquired heart defects.Material and methods. The study included 20 patients with CAD and 18 patients with aortic stenosis/regurgitation. Biopsies of subcutaneous, epicardial, perivascular AT (SCAT, EAT, PVAT, respectively) were obtained during surgery. Quantitative PCR test was used to evaluate the gene expression of de novo ceramide synthesis enzymes (serine palmitoyltransferase C1 and C2: SPTLC1, SPTLC2; ceramide synthase 1-6: CERS1-6; dihydroceramide desaturase: DEGS1). Statistical analysis was performed using GraphPad Prism 8 (GraphPad Software).Results. Patients with CAD were characterized by a higher level of mRNA SPTLC1 in SCAT and EAT, SPTLC2, CERS1, producing C18 ceramides, CERS5 and CERS6, generating C14-C16 ceramides in EAT, CERS2 — in SCAT, producing long-chain ceramides C20-C24, CERS4, synthesizing very long-chain ceamides C18-C20. In PVAT, a high expression of CERS4 and CERS3, which synthesizes very long-chain ceramides C26 and higher, was revealed. DEGS1 expression was highest in SCAT and EAT. In patients with heart defects, there was a high expression of CERS3 in PVAT, CERS4 in EAT and PVAT, DEGS1 in EAT. The mRNA level of SPTLC1 in SCAT and EAT, SPTLC2 in EAT, CERS2 in all studied AT, CERS4 and 5 in EAT, DEGS1 in SCAT and EAT among patients with CAD was higher than in the comparison group.Conclusion. Regional fat depots of the heart differed in the level of expression of enzymes of the ceramide de novo synthesis pathway. The results obtained indicate the activation of ceramide synthesis along this pathway in predominantly epicardial adipocytes in coronary pathology, which may contribute to the accumulation of long-chain ceramides in the AT of this localization.
Effects of Goat Manure, Biochar, and NPK Applications on Growth and Nutrient Concentrations of Lettuce
Authors: MOUNIROU Moustapha Maman , KAYA Emre Can , TAŞKIN Mehmet Burak , İNAL Ali , ABDOUL-AZİZE Hamidou Taffa 
Published: January 2023

During the last decades, biochar (BC) gained increasing importance amongst scholars. Though, only a few studies explored the effects of the combined application of BC with manure on plant growth and nutrient concentrations. This study investigates the effects of separate and joint applications of goat manure (GM) and its derived BC on the growth and mineral element concentrations in lettuce. A completely random design was used in the field experiment. Two factors consisted of GM, BC at 5 Mg ha-1 and 10 Mg ha-1 combined with inorganic fertilizers [nitrogen, phosphorus, and potassium (NPK)] at 100%, 50%, and 0% were applied to the soil before planting the lettuce and, ANOVA analysis and Duncan test (at α=5%) were conducted. The results showed that a joint application of inorganic and organic fertilizers affects significantly the yield and yield parameters of the lettuce whilst the separate application of organic fertilizer affects significantly the lettuce content in N, P, K, Ca and Mg. Compared to GM, the application of GM-derived BC increases significantly the lettuce content in Zn and decreases its content in Cu. In addition, the highest total yield of lettuce was obtained with a joint combination of GM, BC, and 100% NPK (100 kg N ha-1, 100 kg P2O5 ha-1 and 100 kg K2O ha-1). Consequently, this combination of organic and inorganic fertilizers is favorable fertilization in producing lettuce.
Evaluation of the Antioxidant Capacity, Antimicrobial Effect, and In Vitro Digestion Process of Bioactive Compounds of Cherry Laurel Leaves Extracts
Authors: ÖZKAN Kübra , KARADAĞ Ayşe , SAĞDIÇ Osman 
Published: January 2023

Cherry laurel is a less known fruit species with an astringent taste and is mostly consumed as fresh fruit only in the Black Sea and Marmara regions of Turkey. Cherry laurel (Laurocerasus officinalis Roemer) leaves can be prepared in different forms such as infusion by steeping the dried leaf in boiled water and as an extract for its further use as a food supplement or ingredient. In thisstudy, aqueous and ethanol extracts of cherry laurel leaves were prepared and examined in terms of total phenolic compound (TPC), total flavonoid compound (TFC), antioxidant capacities using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and the copper reducing antioxidant capacity (CUPRAC) assays after submitting to in vitro digestion. Additionally, theantimicrobial potential of the leaves extract was evaluated. The TPC of ethanol and aqueous extracts were found at 17.62 and 0.83 mg gallic acid equivalent GAE.g-1 leaves and the TFC of ethanol and aqueous extracts were determined as 11.61 and 0.47 mg catechin equivalent CE.g-1 leaves, respectively. In terms of antioxidant activity results, ethanol and aqueous extracts had 41.11 and 0.77mg Trolox equivalent TE.g-1 leaves for the DPPH assay, and 67.05 and 1.63 mg TE.g-1 leaves for the CUPRAC assay. After gastric digestion post-gastric (PG), compared to the initial values significantly lower recovery of the TPC (11.2 and 41%) and TFC (5.8 and 14.9 %) was observed for ethanol and aqueous extracts. The recovery of TPC, TFC values after the intestinal fraction waslower compared to the PG fraction for ethanol extracts, whereas for aqueous extracts they were higher compared to the PG fraction. The highest inhibition zone was observed against Listeria monocytogenes and Aspergillus niger when 10% extract concentration was applied. The experimental data verified that these extracts displayed remarkable antioxidant and antimicrobial activities, and the extraction method was important in terms of the bioaccessibility of bioactive compounds.
Recent Advances in High Pressure Processing of Milk and Milk Products - A review
Authors: AGRİOPOULOU Sofia , SEVEROĞLU Merve , VARZAKAS Theo , ÖZER Hamdi Barbaros 
Published: January 2023

As the global consumers’ demand towards minimally processed freshlike foods has been continuously increasing, efforts to develop novel food processing technologies have been intensified. Among non-thermal food processing technologies, high pressure processing (HPP) seems to be more advantageous due to its environmentally friendly nature, cost efficiency, suitability for processing foods in any form and its positive impacts on foods’ shelf-life as well as providing efficient microbial safety. Microbiological inactivation efficiency of HPP has been well documented but the role of this technology in digestion efficiency of milk compounds is yet to be elucidated in detail. Also, the potential safety hazards and challenges of HPP in foods require more intense studies. This review deals with the recent developments in HPP treatment to milk and milk products.
Efficient simulation of constantQusing coarse-grained memory variables
Authors: Day Steven M. 
Published: January 2023

AbstractImprovements in computing speed have progressively increased the usable bandwidth of seismic wave-field simulations computed with time-stepped numerical schemes (e.g., finite difference, finite element, pseudospectral). As computational bandwidth increases, anelastic losses become increasingly significant for some important applications such as earthquake ground-motion modeling, whole earth seismogram simulation, and exploration seismic profile modeling, and these losses need to be included in the simulations. As bandwidth increases, however, the memory variables necessary to incorporate realistic anelastic losses account for an increasing proportion of total computational storage requirements, a consequence of the broad relaxation spectrum of typical earth materials. To reduce these storage requirements, we introduce a new method in which the memory variables are coarse grained, that is, redistributed in such a way that only a single relaxation time is represented at each node point (and therefore a single memory variable per stress component is required). Guided by a perturbation analysis, we effect this redistribution in such a way that spatial variability of this single relaxation time simulates the full relaxation spectrum. Such coarse graining reduces memory-variable storage requirements by a factor of 8 for 3D problems or a factor of 4 for 2D problems.In fourth-order finite-difference computations for the 3D acoustic-wave equation, the method simulates frequency-independent Q within a 3% tolerance over 2 decades in frequency, and it is highly accurate and free of artifacts over the entire usable bandwidth of the underlying finite-difference scheme. These results should also hold for the elastodynamic equations. The method is readily generalized to approximate specific frequency-dependent Q models such as power laws or to further reduce memory requirements. In its present implementation, the main limitation of the method is that it generates artifacts at wavelengths equal to 4 grid cell dimensions and shorter, which may, in some limited circumstances, overlap the usable bandwidth of very high-order finite-difference and/or pseudospectral schemes.
Retos y amenazas de la revolución tecnológica en el ámbito de los derechos humanos
Authors: Cardona Rubert María Belén 
Published: January 2023

Las tecnologías digitales abren nuevos escenarios para la vida en sociedad, para la economía y las relaciones interpersonales. Son muchos los retos y desafíos frente a los que nos sitúan la revolución tecnológica como sociedad. Las tecnologías abren nuevos horizontes al papel regulador del Derecho y a la labor de los profesionales del Derecho, llamados a encontrar el equilibrio entre la utilización de las tecnologías y el respeto a los derechos y libertades del individuo. Estamos ante desafíos globales, un cambio de paradigma y de época. Nuevos retos para los que hay que hallar soluciones aterrizadas en la realidad concreta y desde la perspectiva garantista de los derechos. El gran reto es proteger y garantizar el derecho fundamental a la protección de datos en un contexto público-privado de aceleración de la innovación, transformación digital, de fomento de la economía de datos y de generalización de aplicación de la inteligencia artificial, desde una visión holística de la sociedad y la economía y con una perspectiva inclusiva, ética y garante de los derechos humanos.
Morphology of the epithelium of the fallopian tubes of rats under the influence of chronic low-intensity γ-radiation
Authors: Pavlov A. V. , Ermakova O. V. , Korableva T. V. , Fokanova O. A. 
Published: January 2023

The purpose of the study was to investigate the functional morphology of the ciliary transport system of the fallopian tubes of sexually mature rats under the influence of chronic low-intensity γ-radiation.Material and methods. Using a complex technique of intravital microscopic observation, the motor activity of the ciliary apparatus was studied. The frequency of the main types of epithelial cells in the layer was determined on histological sections stained with hematoxylin and eosin. There was also determined morphometry of digitized images of cell structures (cell height, length of cilia) of the epithelial lining of the fallopian tubes of 16 female Wistar rats subjected to experimental chronic γ-irradiation for 55–70 days (total absorbed doses – 5 cGy and 50 cGy) and 8 control animals that were under natural background radiation.Results. The epithelium of the fallopian tubes in the control and experiment in rats contains three types of cells: ciliated, secretory and intercalary (cambial). Compared to the control of the irradiated rats the average height of ciliated cells and the length of cilia were decreased by 30% and 23%, respectively, at a total absorbed dose of 5 cGy and by 16% and 13% at 50 cGy (p 18% (p recorded, while the dimensional characteristics of secretory cells did not significantly differ from the control (p>0,05).Conclusions. The effects of chronic low-intensity γ-radiation on the epithelial lining of the fallopian tubes can be clearly manifested at the level of physiological reactions of cells; irradiation at a minimum dose of 5 cGy is already capable of causing atrophic rearrangements and dysfunction of the ciliated epithelium. The results obtained indicate a high radiosensitivity of the studied tissue elements and make it possible to consider chronic ionizing radiation as a potential risk factor disrupting the structure and function of the epithelial lining of the organ.
Morphofunctional changes in human skin with acneiform dermatoses
Authors: Pustovaya K. N. , Pyavchenko G. A. , Alexeeva N. T. , Kozlov I. O. , Shamitko Z. V. , Kovrigin V. A. , Nozdrin V. I. , Kuznetsov S. L. 
Published: January 2023

The aim of this study was to study the morphofunctional changes in human skin with acneiform dermatoses.Material and methods. We studied the material obtained from 6 volunteers of both sexes aged 26 to 64 years. All subjects had signs of acneiform dermatoses. Pathologically changed areas were studied in comparison with normal skin in the same patient. The subjects were anesthetized and a piece of skin 3×3 mm2 in size was taken with the punch biopsy. After standard histological sample preparation, sections were stained with hematoxylin and eosin. The counting and morphometry of skin cell structures was carried out, the presence of lymphocytes, neutrophils, eosinophils, erythrocytes, vacuolized keratinocytes and their remnants in 6 fields of view in 3 histological slides from each patient was determined from each patient under an Axioskop 2 microscope with a ToupCam TP108500A camera and ToupView software (ToupTek , China). The criterion for the degree of destructive changes in the collagen fibers of the dermis was the determination of their thickness.Results. The morphological structure of the skin in acneiform dermatosis is characterized by a significant increase in the thickness of the spinous (93,7 [82,30; 96,35] microns), granular (28,4 [22,48; 34,44] microns) and corneum (37,8 [25,32; 46,24] microns) layers of the epidermis compared to the norm (66,1 [58,30; 69,42] microns; 6,2 [5,96; 6,63] microns; 10,6 [9,81; 11,26] microns, respectively). Near the sebaceous-follicular complex, inflammatory lymphohistiocytic infiltrates were found in the dermis, represented by lymphocytes (9,5±2,2 units p.f.v.), neutrophils (2,2±0,8 units p.f.v.), eosinophils (17,2±4,0 units p.f.v.), erythrocytes outside the vessels (6,8±1,1 units p.f.v.), vacuolated keratinocytes (22,1±5,7 units p.f.v.) and keratinocyte remnants (15,1±2,4 units p.f.v.) . The number of cells in the infiltrate significantly differed from the norm. On histological preparations of patients with acneiform dermatosis, thinning of the collagen fibers of the dermis (6,0 [4,79; 8,13] microns) was observed compared to unaffected skin (10,4 [10,01; 10,67] microns).Conclusions. Morphological changes in the skin with acneiform dermatosis are manifested by hypertrophic processes of the epidermis, thinning of the collagen fibers of the papillary dermis, the presence of leukocyte infiltrate and a violation of the integrity of the basement membrane of the sebaceous glands. These changes can lead to disruption of the integrity and functioning of the histo-hematic barrier of the skin and an increase in the area of spread of the inflammatory process.
Evaluation of indicators of physical development and quality of life of students of M. K. Ammosov North-Eastern Federal University
Authors: Vinokurova S. P. , Garmaeva D. K. , Arzhakova L. I. , Makarova V. A. , Lytkina A. A. , Garmaev Ts. K. 
Published: January 2023

The aim was to evaluate the indicators of physical development and quality of life in students of M.K. Ammosov North-Eastern Federal University (NEFU).Material and methods. The study involved 755 students of NEFU. at the age of 17–20, Mongoloid and Europeoid races, among which there were 273 (36.16%) girls and 482 (63.84%) boys. The studies were conducted on the basis of voluntary informed written consent. The survey included the determination of anthropometric parameters, assessment of the quality of life and questionnaires. Identification of the main characteristics of students' health was carried out through a medical examination.Results. An analysis of morphological indicators revealed differences in the physical development of students of the Mongoloid and Europeoid races of the Republic of Sakha (Yakutia). In the analyzed groups, there were significant differences in body length depending on race. The highest indicators of body length are determined in persons of the Europeoid race, the smallest values - in students of the Mongoloid race. The study of quality of life indicators found a decrease in the psychological component of health among the respondents, due to the lack of readiness of the components of the professional and personal perspective and the inability to carry out psychological regulation of their behavior, which serves as an obstacle to their adaptation to independent living. At the same time, the indicators of the physical component of the health of the examined students remain, in most cases, within the normal range.Conclusion. Analysis of the results of the study of the physical development of NEFU students indicates that representatives of the Mongoloid race living in the territory of Yakutia are characterized by growth below average, low density, and a small body surface area compared to representatives of the Europeoid race. The level of physical development in most of the examined persons is assessed as average, and in 37.2% of students the functional state of the circulatory system is characterized by tension in the mechanisms of adaptation, which may be due to the adaptation of the body to new living conditions.
Morphological and immunohistochemical validation personalized patient-derived xenograft model of non-small cellular lung cancer
Authors: Epishkina A. A. , Deryabina O. N. , Tumutolova O. N. , Blinov D. S. , Makhrova A. A. , Brykin G. V. , Shimanovskii D. N. , Blinova E. V. 
Published: January 2023

The aim of the study was to carry out a comparative morphological and immunohistochemical analysis of peripheral non-small cell lung cancer, which served as a source of a xenograft tumor, and tissues of the third generation of a tumor that developed in animals.Material and methods. We used 19 athymic BALB/c nu/nu mice, which were intraperitoneally injected with 1×106 CD8+ after sublethal irradiation in accordance with the humanization protocol. The transplantation of a tumor obtained from a 64-year-old patient was carried out three times consecutively. Samples of the original and xenograft tumors were automatically stained with hematoxylin and eosin, rabbit anti-CK7, anti-TTF, and anti-Ki67 antibodies. The evaluation of histological samples was carried out in accordance with the WHO recommendations (2015).Results. It has been established that a third-generation tumor developing in the body of athymic humanized mice retains the morphological and immunohistochemical features of the patient's original tumor. The described approach may be used in preclinical and personalized studies in fundamental pharmacology and molecular oncology.
CHAMPIONS OF UKRAINIAN TRANSLATION STUDIES IN PREŠOV REGION
Authors: LITVINYAK Oleksandra V. 
Published: January 2023

The article aims to present some generalizations on Ukrainian Translation Studies in the Prešov region of Slovakia and introduce the readers with the most prominent figures representing it. The subject of research is the contribution of translation studies scholars of Prešov region to the development of Ukrainian translation studies and popularization of literature translated from and into Ukrainian among Ukrainians in Slovakia. The study of works by the most active translation studies researchers from Prešov writing in Ukrainian resulted in a general description of the main problems raised by them, as well as short personalized characteristics of the ”research style” of each of these figures. The findings have been achieved by applying such methods as analysis, synthesis, contrasting and comparison. Conclusions: The Ukrainian community of Prešov region is represented by a number of talented translation studies scholars who have actively conducted and continue to conduct research on the basis of translations from Ukrainian and into Ukrainian. Unfortunately, their works is little known among the Ukrainian academic and research community, as many of them have been published in regional publications that do not reach Ukraine or reach libraries only in very limited numbers. Nevertheless, these authors analyze many issues relevant to translation studies, such as the impact of one's own creativity on translation, the role of translators in the development of the literary process, translation strategies, methods and approaches, etc. The works by Mykhailo Roman, Mykhailo Molnar, Lyubytsya Babota, Yosyf Shelepets, Mykola Mushynka, Ivan Matsynskyi, Juriy Kundrat, Ivan Yatskanyn, and others deserve close attention and active use by the Ukrainian scholarly community.
ANTONYMY AS A MEANS OF PORTRAITING SOCIAL INEQUALITY IN THE DRAMATIC POEM "IN THE CATACOMBS" BY LESYA UKRAINKA
Authors: STEPANOVA Svitlana Ie. 
Published: January 2023

The purpose of the scientific research, the results of which are described in this article, is to identify and analyze the facts of antonymy, by using which Lesya Ukrainka creates a contractive image of social inequality between persons who are characters in the dramatic poem "In the Catacombs", and to determine the specificity of this language and artistic medium in the author's picture of the world. The object of study are lexemes and replicas that illuminate the social background of relations between representatives of the ancient Roman society during the era of persecution of Christians. The subject of this study is the functioning mechanisms of the revealed antonymic language mediums in the dramatic poem "In the Catacombs" and their role in creating the social space of this work. The methods of sociolinguistic, semantic and contrastive analysis, as well as the descriptive method, are applied. The result of the analysis is the identification of facts of artistic antonymy in the replicas of the characters, primarily the Christian bishop and the neophyte slave, in the dispute about social justice. Some conclusions were made, in particular: antonymy is the leading artistic device of the dramatic poem "In the Catacombs", by using which Lesya Ukrainka highlights the God-loving positions of people who disagree with social inequality in society and are ready to fight for social fairness; antonym pairs in the work are distinguished at the lexical, phraseological and textual levels; the main conflicting antonym pair in this poem is SLAVE ≠ FREE. Socially conditioned antonymous pairs MAN ≠ WOMAN; CHRISTIAN ≠ PAGAN; GOD / KING ≠ HUMAN also intensify the conflict.