bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prognostic significance of a negative PSMA-PET scan prior to salvage radiotherapy following radical prostatectomy
Authors: Adebahr Sonja , Althaus Alexander , Scharl Sophia , Strouthos Iosif , Farolfi Andrea , Serani Francesca , Lanzafame Helena , Trapp Christian , Koerber Stefan A. , Peeken Jan C. , Vogel Marco M. E. , Vrachimis Alexis , Spohn Simon K. B. , Grosu Anca-Ligia , Kroeze Stephanie G. C. , Guckenberger Matthias , Fanti Stefano , Hruby George , Emmett Louise , Belka Claus , Schmidt-Hegemann Nina-Sophie , Henkenberens Christoph , Aebersold Daniel M. , Wiegel Thomas , Afshar-Oromieh Ali , Zamboglou Constantinos , Shelan Mohamed 
Published: September 2023

Abstract
Aim
The optimal management for early recurrent prostate cancer following radical prostatectomy (RP) in patients with negative prostate-specific membrane antigen positron-emission tomography (PSMA-PET) scan is an ongoing subject of debate. The aim of this study was to evaluate the outcome of salvage radiotherapy (SRT) in patients with biochemical recurrence with negative PSMA PET finding.

Methods
This retrospective, multicenter (11 centers, 5 countries) analysis included patients who underwent SRT following biochemical recurrence (BR) of PC after RP without evidence of disease on PSMA-PET staging. Biochemical recurrence-free survival (bRFS), metastatic-free survival (MFS) and overall survival (OS) were assessed using Kaplan-Meier method. Multivariable Cox proportional hazards regression assessed predefined predictors of survival outcomes.

Results
Three hundred patients were included, 253 (84.3%) received SRT to the prostate bed only, 46 (15.3%) additional elective pelvic nodal irradiation, respectively. Only 41 patients (13.7%) received concomitant androgen deprivation therapy (ADT). Median follow-up after SRT was 33 months (IQR: 20–46 months). Three-year bRFS, MFS, and OS following SRT were 73.9%, 87.8%, and 99.1%, respectively. Three-year bRFS was 77.5% and 48.3% for patients with PSA levels before PSMA-PET ≤ 0.5 ng/ml and > 0.5 ng/ml, respectively. Using univariate analysis, the International Society of Urological Pathology (ISUP) grade > 2 (p = 0.006), metastatic pelvic lymph nodes at surgery (p = 0.032), seminal vesicle involvement (p < 0.001), pre-SRT PSA level of > 0.5 ng/ml (p = 0.004), and lack of concomitant ADT (p = 0.023) were significantly associated with worse bRFS. On multivariate Cox proportional hazards, seminal vesicle infiltration (p = 0.007), ISUP score >2 (p = 0.048), and pre SRT PSA level > 0.5 ng/ml (p = 0.013) remained significantly associated with worse bRFS.

Conclusion
Favorable bRFS after SRT in patients with BR and negative PSMA-PET following RP was achieved. These data support the usage of early SRT for patients with negative PSMA-PET findings.
A phase I study of the ceramide nanoliposome in patients with advanced solid tumors
Authors: Ciner Aaron , Gourdin Theodore , Davidson Jeff , Parette Mylisa , Walker Susan J. , Fox Todd E. , Jiang Yixing 
Published: September 2023

Abstract
Purpose
Ceramide is a sphingolipid metabolite that deactivates multiple oncogenic signaling pathways and promotes cell death. In-vivo data demonstrate single-agent anti-cancer activity and enhanced efficacy with combination strategies. This phase I dose-escalation trial evaluated Ceramide nanoLiposomes (CNL) in patients with advanced solid tumors and no standard treatment option.

Methods
The primary objective was to establish the maximum tolerated dose. Secondary objectives included determining the recommended phase II dose, the safety and tolerability, the pharmacokinetic profile and preliminary anti-tumor efficacy.

Results
15 patients with heavily pretreated metastatic disease enrolled. Safety data were analyzed for all patients, while pharmacokinetic data were available for 14 patients. There were no grade 3 or higher treatment-related adverse events. The maximum tolerated dose was not reached and there were no dose-limiting toxicities. The most common grade 1 or 2 treatment-related adverse events included headache, fatigue, constipation, nausea and transaminitis. The maximum concentration and area under the curve increased with dose. Clearance was consistent between doses and was observed mainly through the liver without significant hepatotoxicity. The half-life ranged from 20 to 30 h and the volume of distribution was consistent with a lipophilic drug.

Conclusions
CNL exhibited an encouraging safety profile and pharmacokinetic parameters, with some signals of efficacy including prolonged stable disease in 1 patient with refractory pancreatic cancer. Pre-clinical data indicate potential synergy between CNL and multiple systemic therapies including chemotherapy, targeted therapy, and immunotherapy. Future studies are planned investigating CNL in combination strategies.

Trial registration
This study is registered under ClinicalTrials.gov ID: NCT02834611.
Rapid Detection of blaKPC in Carbapenem-Resistant Enterobacterales Based on CRISPR/Cas13a
Authors: Liang Mingjun , Xiao Bin , Chen Lidan , Huang Xiaoyan , Li Jinchao , Kuang Zhenzhan , Chen Xinping , Huang Xiuna , Sun Zhaohui , Li Linhai 
Published: September 2023

AbstractKlebsiella pneumoniae carbapenemase (KPC) is a crucial enzyme that causes carbapenem resistance in Enterobacterales, and infections by these "superbugs" are extremely challenging to treat. Therefore, there is a pressing need for a rapid and accurate KPC detection test to control the prevalence of carbapenem-resistant Enterobacterales (CREs). In this study, we established a novel method for detection of blaKPC, the gene responsible for encoding KPC, based on a recombinase polymerase amplification (RPA) and a CRISPR/Cas13a reaction coupled to fluorophore activation (termed RPA-Cas13a assay). We carefully selected a pair of optimal amplification primers for blaKPC and achieved a lower limit of detection of approximately 2.5 copies/μL by repeatedly amplifying a recombinant plasmid containing blaKPC. The RPA-Cas13a assay demonstrated a sensitivity of 96.5% and specificity of 100% when tested on 57 blaKPC-positive CRE strains, which were confirmed by DNA sequencing. Moreover, in 311 sputum samples, the theoretical antibiotic resistance characteristics of blaKPC-positive strains obtained by the RPA-Cas13a assay were highly consistent with the results of antibiotic susceptibility test (Kappa = 0.978 > 0.81, P < 0.01). In conclusion, the RPA-Cas13a system is a simple and one-hour efficient technology for the detection of a potentially fatal antibiotic resistance gene.
A novel method of carotid artery wall imaging: black-blood CT
Authors: Lu Yao , Cao Ruoyao , Jiao Sheng , Li Ling , Liu Chao , Hu Hailong , Ma Zhuangfei , Jiang Yun , Chen Juan 
Published: September 2023

Abstract
Objectives
To evaluate the application of black-blood CT (BBCT) in carotid artery wall imaging and its accuracy in disclosing stenosis rate and plaque burden of carotid artery.

Methods
A total of 110 patients underwent contrast-enhanced CT scan with two phases, and BBCT images were obtained using contrast-enhancement (CE)-boost technology. Two radiologists independently scored subjective image quality on black-blood computerized tomography (BBCT) images using a 4-point scale and then further analyzed plaque types. The artery stenosis rate on BBCT was measured and compared with CTA. The plaque burden on BBCT was compared with that on high-resolution intracranial vessel wall MR imaging (VW-MR imaging). The kappa value and intraclass correlation coefficient (ICC) were used for consistency analysis. The diagnostic accuracy of BBCT for stenosis rate and plaque burden greater than 50% was evaluated by AUC.

Results
The subjective image quality scores of BBCT had good consistency between the two readers (ICC = 0.836, p < 0.001). BBCT and CTA had a good consistency in the identification of stenosis rate (p < 0.001). There was good consistency between BBCT and VW-MR in diagnosis of plaque burden (p < 0.001). As for plaque burden over 50%, BBCT had good sensitivity (93.10%) and specificity (73.33%), with an AUC of 0.950 (95%CI 0.838–0.993). Compared with CTA, BBCT had higher consistency with VW-MR in disclosing low-density plaques and mixed plaques (ICC = 0.931 vs 0.858, p < 0.001).

Conclusions
BBCT can not only display the carotid artery wall clearly but also accurately diagnose the stenosis rate and plaque burden of carotid artery.

Clinical relevance statement
Black-blood CT, as a novel imaging technology, can assist clinicians and radiologists in better visualizing the structure of the vessel wall and plaques, especially for patients with contraindication to MRI.

Key Points
• Black-blood CT can clearly visualize the carotid artery wall and plaque burden.
• Black-blood CT is superior to conventional CTA with more accurate diagnosis of the carotid stenosis rate and plaque burden features.
Reconnection of Infinitely Thin Antiparallel Vortices and Coherent Structures
Authors: Iakunin Sergei , Vega Luis 
Published: September 2023

AbstractOne of the characteristic features of turbulent flows is the emergence of many vortices which interact, deform, and intersect, generating a chaotic movement. The evolution of a pair of vortices, e.g., condensation trails of a plane, can be considered as a basic element of a turbulent flow. This simple example nevertheless demonstrates very rich behavior which still lacks a complete explanation. In particular, after the reconnection of the vortices some coherent structures with the shape of a horseshoe emerge. They have a high level of complexity generated by the interaction of waves running from the reconnection region. These structures also turn to be very reminiscent to the ones obtained from the localized induction approximation applied to a polygonal vortex. It can be considered as an evidence that a pair of vortices creates a corner singularity during the reconnection. In this work we focus on a study of the reconnection phenomena and the emerged structures. In order to do it we present a new model based on the approximation of an infinitely thin vortex, which allows us to focus on the chaotic movement of the vortex center line. The main advantage of the developed model consists in the ability to go beyond the reconnection time and to see the coherent structures. It is also possible to define the reconnection time by analyzing the fluid impulse.
Hysteroscopic versus histopathological agreement in the diagnosis of chronic endometritis: results from a retrospective observational study
Authors: Pérez-Cejuela Belén Almoguera , Vitale Salvatore Giovanni , Pérez-Medina Tirso , Rios-Vallejo Mar , Della Corte Luigi , Vicente Ana Royuela , Angioni Stefano , Calles-Sastre Laura 
Published: September 2023

Abstract
Purpose
To evaluate the agreement rate between hysteroscopy and pathological examination in case of chronic endometritis.

Methods
A retrospective observational study carried out at Gynecology and Obstetrics Department, Puerta de Hierro Hospital, Autónoma University of Madrid, Spain, from January 2021 to June 2022 was performed by obtaining data from 115 medical records of women who underwent office hysteroscopies that was compared with the findings of final histological examination of endometrial biopsy. Cohen's kappa index was used to evaluate this agreement rate. In addition, sensitivity, specificity, positive and negative predictive value and diagnostic accuracy were obtained.

Results
The agreement between hysteroscopic findings and histological examination showed a modest result with a Cohen’s kappa index of 34%. In addition, we obtained a specificity of 70% and a sensitivity of 64%. The positive and negative predictive value were 60.8% and 73.4%, respectively. An excellent agreement rate (100%) between histological and hysteroscopic results was observed in presence of hyperemia and micropolyps.

Conclusion
Although the sample size is not as large as that of other studies published so far, the first glance of our experience is that hysteroscopic signs are not yet sufficient to make an accurate diagnosis of chronic endometritis, thus requiring a histopathological confirmation to make it.
Lap. Nissen fundoplication leads to better respiratory symptom control than Toupet in the long term of 20 years
Authors: Gehwolf Philipp , Agerer Teresa , Stacul Nadine , Kienzl-Wagner Katrin , Schäfer Aline , Berchtold Valeria , Cakar-Beck Fergül , Elisabeth Gasser , Wykypiel Heinz 
Published: September 2023

Abstract
Introduction
Having performed anti-reflux surgery for thirty years, it was important to reexamine our patients in the long term to enlarge the body of evidence concerning classical and extraesophageal symptoms that are differently controlled by Nissen or Toupet fundoplication.

Objectives
We report a cohort of 155 GERD patients who underwent fundoplication within a tailored approach between 1994 and 2000. Changes in the perioperative functional outcome, GERD symptoms, and quality of life are being analyzed 10 and 20 years after the operation.

Results
The operation resulted in a superior quality of life compared to a patient cohort treated with PPI therapy. We found that both surgical methods (laparoscopic Nissen fundoplication and laparoscopic Toupet fundoplication) cure classical symptoms equally (heartburn, regurgitation, and dysphagia). GERD patients receiving a Toupet fundoplication seem more likely to suffer from extraesophageal GERD symptoms 10 and 20 years after surgery than patients with a Nissen fundoplication. On the other hand, some patients with Nissen fundoplication report dysphagia even 10 and 20 years after surgery.

Conclusion
Both the laparoscopic Nissen and Toupet fundoplications provide excellent symptom control in the long term. Moreover, the Nissen fundoplication seems to be superior in controlling extraesophageal reflux symptoms, but at the expense of dysphagia. In summary, tailoring the operation based on symptoms seems advantageous.
Integrative landscape analysis of prognostic model biomarkers and immunogenomics of disulfidptosis-related genes in breast cancer based on LASSO and WGCNA analyses
Authors: Liu Shuyan , Zheng Yiwen , Li Shujin , Du Yaoqiang , Liu Xiaozhen , Tang Hongchao , Meng Xuli , Zheng Qinghui 
Published: September 2023

Abstract
Background
Disulfidptosis is a novel type of programmed cell death. However, the value of disulfidptosis-related genes (DRGs) in the prediction of breast cancer prognosis is unclear.

Methods
RNA-seq data of 1231 patients, together with information on patient clinical characteristics and prognosis, were downloaded from TCGA. DRGs were identified between cancerous and non-cancerous tissues. The LASSO algorithm was used to assign half of the samples to the training set. Risk scores were used for construction of a prognostic model for risk stratification and prognosis prediction, and the clinical applicability was examined using a line diagram. The relationships between risk scores, immune cell infiltration, molecular subtypes, and responses to immunotherapy and chemotherapy were examined.

Results
We identified and obtained four DRG-related prognostic lncRNAs (AC009097.2, AC133552.5, YTHDF3-AS1, and AC084824.5), which were used for establishing the risk model. Longer survival was associated with low risk. The DRG-associated lncRNAs were found to independently predict patient prognosis. The AUCs under the ROCs for one-, three-, and 5-year survival in the training cohort were 0.720, 0.687, and 0.692, respectively. The model showed that the high-risk patients had reduced overall survival as well as high tumor mutation burdens. Furthermore, high-risk patients showed increased sensitivity to therapeutic drugs, including docetaxel, paclitaxel, and oxaliplatin.

Conclusion
The risk score model was effective for predicting both prognosis and sensitivity to therapeutic drugs, suggesting its possible usefulness for the management of patients with breast cancer.
The impact of β-glucan on the therapeutic outcome of experimental Trichinella spiralis infection
Authors: Elgendy Dina I. , Othman Ahmad A. , Eid Mohamed M. , El-Kowrany Samy I. , Sallam Fersan A. , Mohamed Dareen A. , Zineldeen Doaa. H. 
Published: September 2023

AbstractTrichinellosis is a cosmopolitan zoonosis that is caused mainly byTrichinella spiralisinfection. The human disease ranges from mild to severe and fatality may occur. The treatment of trichinellosis still presents a challenge for physicians. Anti-inflammatory drugs are usually added to antiparasitic agents to alleviate untoward immuno-inflammatory responses and possible tissue damage but they are not without adverse effects. Thus, there is a need for the discovery of safe and effective compounds with anti-inflammatory properties. This study aimed to evaluate the activity of β-glucan during enteral and muscular phases of experimentalT. spiralisinfection as well as its therapeutic potential as an adjuvant to albendazole in treating trichinellosis. For this aim, mice were infected withT. spiralisand divided into the following groups: early and late β-glucan treatment, albendazole treatment, and combined treatment groups. Infected mice were subjected to assessment of parasite burden, immunological markers, and histopathological changes in the small intestines and muscles. Immunohistochemical evaluation of NF-κB expression in small intestinal and muscle tissues was carried out in order to investigate the mechanism of action of β-glucan. Interestingly, β-glucan potentiated the efficacy of albendazole as noted by the significant reduction of counts of muscle larvae. The inflammatory responses in the small intestine and skeletal muscles were mitigated with some characteristic qualitative changes. β-glucan also increased the expression of NF-κB in tissues which may account for some of its effects. In conclusion, β-glucan showed a multifaceted beneficial impact on the therapeutic outcome ofTrichinellainfection and can be regarded as a promising adjuvant in the treatment of trichinellosis.
From ductile damage to unilateral contact via a point-wise implicit discontinuity
Authors: Daneshyar Alireza , Herrmann Leon , Kollmannsberger Stefan 
Published: September 2023

AbstractDuctile damage models and cohesive laws incorporate the material plasticity entailing the growth of irrecoverable deformations even after complete failure. This unrealistic growth remains concealed until the unilateral effects arising from the crack closure emerge. We address this issue by proposing a new strategy to cope with the entire process of failure, from the very inception in the form of diffuse damage to the final stage, i.e. the emergence of sharp cracks. To this end, we introduce a new strain field, termed discontinuity strain, to the conventional additive strain decomposition to account for discontinuities in a continuous sense so that the standard principle of virtual work applies. We treat this strain field similar to a strong discontinuity, yet without introducing new kinematic variables and nonlinear boundary conditions. In this paper, we demonstrate the effectiveness of this new strategy at a simple ductile damage constitutive model. The model uses a scalar damage index to control the degradation process. The discontinuity strain field is injected into the strain decomposition if this damage index exceeds a certain threshold. The threshold corresponds to the limit at which the induced imperfections merge and form a discrete crack. With three-point bending tests under pure mode I and mixed-mode conditions, we demonstrate that this augmentation does not show the early crack closure artifact which is wrongly predicted by plastic damage formulations at load reversal. We also use the concrete damaged plasticity model provided in Abaqus commercial finite element program for our comparison. Lastly, a high-intensity low-cycle fatigue test demonstrates the unilateral effects resulting from the complete closure of the induced crack.
Geschlechtsspezifische Ergebnisse des Dresdner Kinder- und Jugendkopfschmerzprogrammes DreKiP
Authors: Zaranek Laura , Sobe Hanna , Richter Matthias , Hübler Anke , Berner Reinhard , von der Hagen Maja , Koch Thea , Sabatowski Rainer , Klimova Anna , Goßrau Gudrun 
Published: September 2023

Zusammenfassung
Hintergrund
Mädchen und Frauen sind häufiger von Kopfschmerzen betroffen als Jungen und Männer. Der Einfluss des Geschlechts auf die Wirksamkeit von Kopfschmerztherapien ist bisher kaum untersucht. Wir prüften geschlechterspezifische Unterschiede im ambulanten multimodalen Dresdner Kinder- und Jugendkopfschmerzprogramm DreKiP.

Methoden
140 Patienten mit primären Kopfschmerzen wurden in einem 15-stündigen strukturiertem Gruppenprogramm behandelt. Zu Beginn des Programms (T0) sowie 6 (T1) und 12 Monate (T2) nach dem Ende wurden Daten zu kopfschmerzbedingter Einschränkung der Alltagsfähigkeit (PedMIDAS), Kopfschmerzfrequenz, -intensität und schmerzbedingter Alltagseinschränkung (P-PDI) erhoben. Retrospektiv wurden diese Daten für Mädchen und Jungen getrennt analysiert.

Ergebnisse
Von 91 Patienten (9–19 Jahre, Median = 15; 71,4 % weiblich) lagen Daten für mindestens zwei Messzeitpunkte vor.
Mädchen zeigten zu allen Zeitpunkten eine signifikant höhere Kopfschmerzfrequenz als Jungen (Mediane Kopfschmerztage/letzte 3 Monate zu T0: ♀ 43, ♂ 20; T1: ♀ 32, ♂ 12; T2: ♀ 28, ♂ 9) sowie eine numerisch höhere kopfschmerzbedingte Alltagseinschränkung. Es zeigten sich signifikante Effekte über die Zeit mit Abnahme der Kopfschmerzfrequenz (F (2,88) = 5,862; p = 0,004) und Verbesserung der Alltagsfunktion (F (2,92) = 5,340; p = 0,006).
Eine geschlechtsspezifische Therapieantwort zeigte sich nicht.

Diskussion
Therapieinhalte des DreKiP zeigten bei Mädchen und Jungen mit primären Kopfschmerzen Effekte. Höhere Kopfschmerzfrequenzen und Alltagseinschränkung bei Mädchen können vor allem hormonelle, aber auch psychosoziale Ursachen haben und sollten in Edukationsmaßnahmen aufgegriffen werden.
User experiences with simulated cyber-physical attacks on smart home IoT
Authors: Huijts N. M. A. , Haans A. , Budimir S. , Fontaine J. R. J. , Loukas G. , Bezemskij A. , Oostveen A. , Filippoupolitis A. , Ras I. , IJsselsteijn W. A. , Roesch E. B. 
Published: September 2023

AbstractWith the Internet of Things (IoT) becoming increasingly prevalent in people’s homes, new threats to residents are emerging such as the cyber-physical attack, i.e. a cyber-attack with physical consequences. In this study, we aimed to gain insights into how people experience and respond to cyber-physical attacks to their IoT devices. We conducted a naturalistic field experiment and provided 9 Dutch and 7 UK households, totalling 18 and 13 participants respectively, with a number of smart devices for use in their home. After a period of adaptation, simulated attacks were conducted, leading to events of varying noticeability (e.g., the light going on or off once or several times). After informing people simulated attacks had occurred, the attacks were repeated one more time. User experiences were collected through interviews and analysed with thematic analyses. Four relevant themes were identified, namely (1) the awareness of and concern about privacy and security risks was rather low, (2) the simulated attacks made little impression on the participants, (3) the participants had difficulties with correctly recognizing simulated attacks, and (4) when informed about simulated attacks taking place; participants noticed more simulated attacks and presented decision rules for them (but still were not able to identify and distinguish them well—see Theme 3). The findings emphasise the need for training interventions and an intrusion detection system to increase detection of cyber-physical attacks.