bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trial‐by‐trial fluctuations of pre‐stimulus alpha power predict language ERPs
Authors: Lago Sara , Pezzetta Rachele , Gastaldon Simone , Peressotti Francesca , Arcara Giorgio 
Published: July 2023

AbstractAnticipatory mechanisms are known to play a key role in language, but they have been mostly investigated with violation paradigms, which only consider what happens after predictions have been (dis)confirmed. Relatively few studies focused on the pre‐stimulus interval and found that stronger expectations are associated with lower pre‐stimulus alpha power. However, alpha power also fluctuates spontaneously, in the absence of experimental manipulations; and in the attention and perception domains, spontaneously low pre‐stimulus power is associated with better behavioral performance and with event‐related potential (ERPs) with shorter latencies and higher amplitudes. Importantly, little is known about the role of alpha fluctuations in other domains, as it is in language. To this aim, we investigated whether spontaneous fluctuations in pre‐stimulus alpha power modulate language‐related ERPs in a semantic congruence task. Electrophysiology data were analyzed using Generalized Additive Mixed Models to model nonlinear interactions between pre‐stimulus alpha power and EEG amplitude, at the single‐trial level. We found that the N400 and the late posterior positivity/P600 were larger in the case of lower pre‐stimulus alpha power. Still, while the N400 was observable regardless of the level of pre‐stimulus power, a late posterior positivity/P600 effect was only observable for low pre‐stimulus alpha power. We discuss these findings in light of the different, albeit connected, functional interpretations of pre‐stimulus alpha and the ERPs according to both a nonpredictive interpretation focused on attentional mechanisms and under a predictive processing framework.
Assessment of the genomic stability of calves obtained from artificial insemination and OPU/IVP in vitro fertilization
Authors: Kępka Katarzyna , Wójcik Ewa , Wysokińska Anna 
Published: July 2023

AbstractDamage to genetic material and errors in the functioning of cellular mechanisms disturb genome stability and integrity. Assessment of genomic stability in animals is a very important aspect of breeding work. Unfortunately, harmful instabilities affect the functioning, health and reproductive processes of animals. Obtaining healthy calves is a priority, whatever methods of reproductive biotechnology are applied. The aim of the study was to assess the genomic stability of calves obtained from artificial insemination and OPU/IVP in vitro fertilization. The genomic stability of the calves was evaluated using the comet, sister chromatid exchange, and fragile sites assays. Damage to the genetic material of calves obtained by two reproductive biotechnologies was identified. Identification of instability in animals can be a valuable tool in breeding work and accelerate breeding progress.
From Reliability-Based Design to Resilience-Based Design
Authors: Wang Cao , Ayyub Bilal M. , Beer Michael 
Published: July 2023

Abstract
Reliability-based design has been a widely used methodology in the design of engineering structures. For example, the structural design standards in many countries have adopted the load and resistance factor design (LRFD) method. In recent years, the concept of resilience-based design has emerged, which additionally takes into account the posthazard functionality loss and recovery process of a structure. Under this context, the following questions naturally arise: can we establish a linkage between reliability-based design and resilience-based design? Does there exist a simple resilience-based design criterion that takes a similar form of LRFD? This paper addresses these questions, and the answer is “yes”. To this end, a new concept of structural resilience capacity is proposed, which is a generalization of structural load bearing capacity (resistance). The probabilistic characteristics (mean value, variance, probability distribution function) of resilience capacity are derived. Applying the concept of resilience capacity, this paper explicitly shows the relationship between the following four items: time-invariant reliability-, time-invariant resilience-, time-dependent reliability-, and time-dependent resilience-based design methods. Furthermore, an LRFD-like design criterion is proposed for structural resilience-based design, namely, load and resilience capacity factor design (LRCFD), whose applicability is demonstrated through an example. The LRCFD method can also be used, in conjunction with LRFD, to achieve reliability and resilience goals simultaneously of the designed structure.
War-related sexual and gender-based violence in Tigray, Northern Ethiopia: a community-based study
Authors: Fisseha Girmatsion , Gebrehiwot Tesfay Gebregzabher , Gebremichael Mengistu Welday , Wahdey Shishay , Meles Gebrekiros Gebremichael , Gezae Kebede Embaye , Legesse Awol Yemane , Asgedom Akeza Awealom , Tsadik Mache , Woldemichael Abraha , Gebreyesus Aregawi , Abebe Haftom Temesgen , Haile Yibrah Alemayehu , Gezahegn Selome , Aregawi Maru , Berhane Kiros T , Godefay Hagos , Mulugeta Afework 
Published: July 2023

IntroductionSexual and gender-based violence (SGBV) during armed conflicts has serious ramifications with women and girls disproportionally affected. The impact of the conflict that erupted in November 2020 in Tigray on SGBV is not well documented. This study is aimed at assessing war-related SGBV in war-affected Tigray, Ethiopia.MethodsA community-based survey was conducted in 52 (out of 84) districts of Tigray, excluding its western zone and some districts bordering Eritrea due to security reasons. Using a two-stage multistage cluster sampling technique, a total of 5171 women of reproductive age (15–49 years) were randomly selected and included in the study. Analysis used weighted descriptive statistics, regression modelling and tests of associations.ResultsOverall, 43.3% (2241/5171) of women experienced at least one type of gender-based violence. The incidents of sexual, physical and psychological violence, and rape among women of reproductive age were found to be 9.7% (500/5171), 28.6% (1480/5171), 40.4% (2090/5171) and 7.9% (411/5171), respectively. Of the sexual violence survivors, rape accounted for 82.2% (411/500) cases, of which 68.4% (247) reported being gang raped. Young women (aged 15–24 years) were the most affected by sexual violence, 29.2% (146/500). Commonly reported SGBV-related issues were physical trauma, 23.8% (533/2241), sexually transmitted infections, 16.5% (68/411), HIV infection, 2.7% (11/411), unwanted pregnancy, 9.5% (39/411) and depression 19.2% (431/2241). Most survivors (89.7%) did not receive any postviolence medical or psychological support.ConclusionsSystemic war-related SGBV was prevalent in Tigray, with gang-rape as the most common form of sexual violence. Immediate medical and psychological care, and long-term rehabilitation and community support for survivors are urgently needed and recommended.
Bacteriochlorin syntheses - Status, problems, and exploration
Authors: Tran Vy-Phuong , Wang Pengzhi , Matsumoto Nobuyuki , Liu Sijia , Jing Haoyu , Nalaoh Phattananawee , Chau Nguyen Khiem , Taniguchi Masahiko , Lindsey Jonathan S. 
Published: July 2023

Bacteriochlorins – Nature’s near-infrared (NIR) chromophores – are distinguished by an intense ([Formula: see text] ∼;105 M[Formula: see text]cm[Formula: see text] long-wavelength absorption band in the ∼;700–1000 nm. The development of routes to prepare synthetic, tailorable bacteriochlorins holds promise for multiple disciplines where NIR-light-promoted photoactivity is of interest. A de novo route to bacteriochlorins equipped with a stabilizing gem-dimethyl group in each pyrroline ring was discovered in 2003. Continued development in this arena over 20 years has led to additional routes as well as methods to install substituents at selected positions about the perimeter of the macrocycle. The present paper reports studies that highlight substantial limitations of existing synthetic routes, including stymied access to multi-bacteriochlorin arrays and the inability to install (in a rational way) distinct groups at opposite sides of the macrocycle. The origins of the limitations are traced to particular stages of the chemistry ranging from derivatizing pyrroles, creating pyrrolines, constructing and elaborating dihydrodipyrrins, coupling dihydrodipyrrins, and forming macrocycles. Through exploration of a dozen aspects of bacteriochlorin syntheses, 60 new compounds (and nine known compounds via improved syntheses) have been prepared and characterized; the data include 20 single-crystal X-ray diffraction analyses. The research taken together points to areas of focus to fulfill the promise of this fascinating class of compounds.
A Novel Role of Prostate-Specific Membrane Antigen in Telomere Stability in Prostate Cancer Cells
Authors: Reddy Vidyavathi , Hwang Clara , Reddy G. Prem-Veer , Kim Sahn-Ho 
Published: July 2023

Abstract

Prostate-specific membrane antigen (PSMA) expression increases with prostate cancer grade and progression; however, the role of PSMA in prostate cancer progression remains poorly understood. Telomere stability is essential for the survival and genome stability of cancer cells. We found massive telomere DNA damage in PSMA-negative prostate cancer cells (PC-3 and DU145) compared with PSMA-positive prostate cancer (LNCaP) cells. The ectopic expression of PSMA suppressed telomere DNA damage in PC3 cells. PSMA inhibitor, 2-PMPA, and PSMA knockdown induced telomere DNA damage in PSMA-positive LNCaP cells but not in PSMA-negative PC-3 cells, suggesting that PSMA plays a critical role in telomere stability in prostate cancer cells. In addition, we observed that inhibition of PSMA or inhibition of glutamate receptor, which mediates PSMA-dependent activation of AKT, suppressed AKT phosphorylation, and caused telomere DNA damage. Furthermore, 2-PMPA-induced telomere DNA damage in LNCaP cells was associated with telomere aberrations, such as telomere–telomere fusions, sister–chromatid telomere fusions, and telomere breakages. AKT is reported to promote cell growth by stabilizing telomere association with telomere-binding proteins TRF1 and TPP1. We observed that TRF1 and TPP1 transfection of LNCaP cells attenuated the inhibitory effect of 2-PMPA on cell growth and telomere DNA damage. Together, these observations indicate that PSMA role in maintaining telomere stability in prostate cancer cells is mediated by AKT. Thus, these studies reveal an important role of PSMA in maintaining telomere stability that can promote cell survival and, thereby, prostate cancer progression.

Implications:
Role of PSMA in telomere stability suggests a strong correlation between PSMA expression and prostate cancer progression.
Combinatorial Immunotherapy with Agonistic CD40 Activates Dendritic Cells to Express IL12 and Overcomes PD-1 Resistance
Authors: Krykbaeva Irina , Bridges Kate , Damsky William , Pizzurro Gabriela A. , Alexander Amanda F. , McGeary Meaghan K. , Park Koonam , Muthusamy Viswanathan , Eyles James , Luheshi Nadia , Turner Noel , Weiss Sarah A. , Olino Kelly , Kaech Susan M. , Kluger Harriet M. , Miller-Jensen Kathryn , Bosenberg Marcus 
Published: July 2023

Abstract
Checkpoint inhibitors have revolutionized cancer treatment, but resistance remains a significant clinical challenge. Myeloid cells within the tumor microenvironment can modulate checkpoint resistance by either supporting or suppressing adaptive immune responses. Using an anti–PD-1–resistant mouse melanoma model, we show that targeting the myeloid compartment via CD40 activation and CSF1R blockade in combination with anti–PD-1 results in complete tumor regression in a majority of mice. This triple therapy combination was primarily CD40 agonist-driven in the first 24 hours after therapy and showed a similar systemic cytokine profile in human patients as was seen in mice. Functional single-cell cytokine secretion profiling of dendritic cells (DC) using a novel microwell assay identified a CCL22+CCL5+ IL12-secreting DC subset as important early-stage effectors of triple therapy. CD4+ and CD8+ T cells are both critical effectors of treatment, and systems analysis of single-cell RNA sequencing data supported a role for DC-secreted IL12 in priming T-cell activation and recruitment. Finally, we showed that treatment with a novel IL12 mRNA therapeutic alone was sufficient to overcome PD-1 resistance and cause tumor regression. Overall, we conclude that combining myeloid-based innate immune activation and enhancement of adaptive immunity is a viable strategy to overcome anti–PD-1 resistance.
Suppression of Heart Failure With PAR1 Pepducin Technology in a Pressure Overload Model in Mice
Authors: Fletcher Elizabeth K. , Ngwenyama Njabulo , Nguyen Nga , Turner Susan E. , Covic Lidija , Alcaide Pilar , Kuliopulos Athan 
Published: July 2023

BACKGROUND:
PAR1 (protease-activated receptor-1) contributes to acute thrombosis, but it is not clear whether the receptor is involved in deleterious inflammatory and profibrotic processes in heart failure. Here, we employ the pepducin technology to determine the effects of targeting PAR1 in a mouse heart failure with reduced ejection fraction model.

METHODS:
After undergoing transverse aortic constriction pressure overload or sham surgery, C57BL/6J mice were randomized to daily sc PZ-128 pepducin or vehicle, and cardiac function, inflammation, fibrosis, and molecular analyses conducted at 7 weeks

RESULTS:
After 7 weeks of transverse aortic constriction, vehicle mice had marked increases in macrophage/monocyte infiltration and fibrosis of the left ventricle as compared with Sham mice. PZ-128 treatment significantly suppressed the inflammatory cell infiltration and cardiac fibrosis. Despite no effect on myocyte cell hypertrophy, PZ-128 afforded a significant reduction in overall left ventricle weight and completely protected against the transverse aortic constriction-induced impairments in left ventricle ejection fraction. PZ-128 significantly suppressed transverse aortic constriction-induced increases in an array of genes involved in myocardial stress, fibrosis, and inflammation.

CONCLUSIONS:
The PZ-128 pepducin is highly effective in protecting against cardiac inflammation, fibrosis, and loss of left ventricle function in a mouse model.
Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells
Authors: Zhang Zijie , Cui Shuyue , Fu Yajing , Wang Jixiao , Liu Jiani , Wei Fulan 
Published: July 2023

Abstract
Background
The preference for glucose oxidative mode has crucial impacts on various physiological activities, including determining stem cell fate. External mechanical factors can play a decisive role in regulating critical metabolic enzymes and pathways of stem cells. Periodontal ligament stem cells (PDLSCs) are momentous effector cells that transform mechanical force into biological signals during the reconstruction of alveolar bone. However, mechanical stimuli-induced alteration of oxidative characteristics in PDLSCs and the underlying mechanisms have not been fully elucidated.

Methods
Herein, we examined the expression of LDH and COX4 by qRT-PCR, western blot, immunohistochemistry and immunofluorescence. We detected metabolites of lactic acid and reactive oxygen species for functional tests. We used tetramethylrhodamine methyl ester (TMRM) staining and a transmission electron microscope to clarify the mitochondrial status. After using western blot and immunofluorescence to clarify the change of DRP1, we further examined MFF, PINK1, and PARKIN by western blot. We used cyclosporin A (CsA) to confirm the regulation of mitophagy and ceased the stretching as a rescue experiment.

Results
Herein, we ascertained that mechanical force could increase the level of LDH and decrease the expression of COX4 in PDLSCs. Simultaneously, the yield of reactive oxygen species (ROS) in PDLSC reduced after stretching, while lactate acid augmented significantly. Furthermore, mitochondrial function in PDLSCs was negatively affected by impaired mitochondrial membrane potential (MMP) under mechanical force, and the augment of mitochondrial fission further induced PRKN-dependent mitophagy, which was confirmed by the rescue experiments via blocking mitophagy. As a reversible physiological stimulation, the anaerobic preference of PDLSCs altered by mechanical force could restore after the cessation of force stimulation.

Conclusions
Altogether, our study demonstrates that PDLSCs under mechanical force preferred anaerobic oxidation induced by the affected mitochondrial dynamics, especially mitophagy. Our findings support an association between mechanical stimulation and the oxidative profile of stem cells, which may shed light on the mechanical guidance of stem cell maintenance and commitment, and lay a molecular foundation for periodontal tissue regeneration.

Graphical Abstract
Associations of circulating fatty acids with incident coronary heart disease: a prospective study of 89,242 individuals in UK Biobank
Authors: Jin Danyao , Trichia Eirini , Islam Nazrul , Lewington Sarah , Lacey Ben 
Published: July 2023

Abstract
Background
The role of fatty acids in coronary heart disease (CHD) remains uncertain. There is little evidence from large-scale epidemiological studies on the relevance of circulating fatty acids levels to CHD risk. This study aims to examine the independent associations of the major circulating types of fatty acids with CHD risk.

Methods
UK Biobank is a prospective study of adults aged 40–69 in 2006–2010; in 2012–2013, a subset of the participants were resurveyed. Analyses were restricted to 89,242 participants with baseline plasma fatty acids (measured using nuclear magnetic resonance spectroscopy) and without prior CHD. Cox proportional hazards models were used to estimate hazard ratios (HRs) for the associations with incidence CHD, defined as the first-ever myocardial infarction, unstable angina pectoris, coronary-related death, or relevant procedure. And the major types of fatty acids were mutually adjusted to examine the independent associations. Hazard ratios were corrected for regression dilution using the correlation of baseline and resurvey fatty acids measures.

Results
During a median follow-up of 11.8 years, 3,815 incident cases of CHD occurred. Independently of other fatty acids, CHD risk was positively associated with saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA), inversely associated with omega-3 polyunsaturated fatty acids (PUFA), but there was no strong evidence of an association with omega-6 PUFA: HR per standard deviation higher were 1.14 (95% CI, 1.09–1.20), 1.15 (1.10–1.21), 0.91 (0.87–0.94), and 1.04 (0.99–1.09) respectively. Independently of triglycerides and cholesterol, the inverse association with omega-3 PUFA was not materially changed, but the positive associations with SFA and MUFA attenuated to null after adjusting for triglycerides levels.

Conclusions
This large-scale study has quantitated the independent associations of circulating fatty acids with CHD risk. Omega-3 PUFA was inversely related to CHD risk, independently of other fatty acids and major lipid fractions. By contrast, independently of other fatty acids, the positive associations of circulating SFA and MUFA with CHD risk were mostly attributed to their relationship with triglycerides.
Spontaneous massive fetomaternal hemorrhage: two case reports and a literature review of placental pathology
Authors: Zheng Yushuang , Li Donglu , Li Xinran , Zheng Aman , Wang Fan 
Published: July 2023

Abstract
Background
Massive fetomaternal hemorrhage (FMH) is a rare event during pregnancy that may cause severe fetal anemia or death.

Case presentation
This paper reports two cases of fetomaternal hemorrhage with unexplained reasons. Both cases required emergency caesarean sections for non-reassuring fetal status and were treated with neonatal blood transfusion. Fetomaternal hemorrhage was confirmed via maternal Kleihauer-Betke test.

Conclusion
We found parenchymal pallor, increased nucleated red blood cells (nRBCs), and syncytial knots (SKs) in the placentas, which are compatible with fetal anemia. Immunohistochemical staining indicated VEGF, CD34, and CD31 expression in the endothelial cells of the capillaries, characteristic of massive FMH placenta. This article also reviews the particular histopathological changes in FHM placenta according to the placental lesion classification system.
Downregulation of MAL2 inhibits breast cancer progression through regulating β-catenin/c-Myc axis
Authors: An Lijun , Gong Huiyuan , Yu Xiaojing , Zhang Wangming , Liu Xiaohua , Yang Xiaomin , Shu Liping , Liu Jielin , Yang Liuqi 
Published: July 2023

Abstract
Purpose
Myelin and lymphocyte protein 2 (MAL2) is mainly involved in endocytosis under physiological conditions and mediates the transport of materials across the membranes of cell and organelle. It has been reported that MAL2 is significantly upregulated in diverse cancers. This study aimed to investigate the role of MAL2 in breast cancer (BC).

Methods
Bioinformatics analysis and Immunohistochemical assay were applied to detect the correlation between MAL2 expression in breast cancer tissues and the prognosis of breast cancer patients. Functional experiments were carried out to investigate the role of MAL2 in vitro and in vivo. The molecular mechanisms involved in MAL2-induced β-catenin and c-Myc expression and β-catenin/c-Myc-mediated enhancement of BC progression were confirmed by western blot, β-catenin inhibitor and agonist, Co-IP and immunofluorescence colocalization assays.

Results
Results from the cancer genome atlas (TCGA) and clinical samples confirmed a significant upregulation of MAL2 in BC tissues than in adjacent non-tumor tissues. High expression of MAL2 was associated with worse prognosis. Functional experiments demonstrated that MAL2 knockdown reduced the migration and invasion associating with EMT, increased the apoptosis of BC cells in vitro and reduced the metastatic capacity in vivo. Mechanistically, MAL2 interacts with β-catenin in BC cells. MAL2 silencing reduced the expression of β-catenin and c-Myc, while the β-catenin agonist SKL2001 partially rescued the downregulation of c-Myc and inhibition of migration and invasion caused by MAL2 knockdown in BC cells.

Conclusion
These observations provided evidence that MAL2 acted as a potential tumor promoter by regulating EMT and β-catenin/c-Myc axis, suggesting potential implications for anti-metastatic therapy for BC.