bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary study on fluid bolus administration for prevention of spinal hypotension in dogs undergoing elective cesarean section
Authors: Antończyk Agnieszka , Kiełbowicz Zdzisław , Niżański Wojciech , Ochota Małgorzata 
Published: March 2023

IntroductionThis study aimed to investigate the effect of fluid bolus administration during epidural anesthesia (coload) in female dogs scheduled for elective cesarean section (CS). Hypotension is one of the most common complications of epidural (EA) and spinal (SA) analgesia, and in the case of cesarean section, it may pose a significant risk for placental perfusion and subsequent fetal vitality and puppy survival.MethodsPregnant bitches scheduled for elective CS underwent EA with (treatment group) or without (control group) intravenous fluid bolus administration. The following parameters were measured and compared between both groups: HR, RR, etCO2, SpO2, systolic, diastolic and mean arterial blood pressure were measured at three time points (T1: before surgery, T2: after the last puppy removal, and T3: end of surgery) in dams; vitality (Apgar score at 0, 5, and 20 min) and umbilical cord blood parameters (pH, pCO2, HCO3, base excess, lactate and glucose) in newborns.ResultsThe results indicated that crystalloid coloading increased maternal systolic, diastolic, and mean blood pressure (treatment vs. control group, 101.46 ± 9.18, 48.01 ± 13.47, and 67.07 ± 13.15 mmHg vs. 80.68 ± 7.29, 36.52 ± 8.75, and 180 52.30 ± 7.77, p &lt; 0.05) with significantly fewer episodes of hypotension. Additionally, puppies in the treatment group received higher scores in the 5-min (7.91 ± 1.67 vs. 6.74 ± 2.20) and 20-min (9.38 ± 0.87 vs. 8.39 ± 2.50) assessments without the favorable effect on umbilical blood gas parameters.DiscussionBased on the obtained results, it can be stated that crystalloid coload offers an effective option in cases of hypotension during cesarean section, with clear benefits for both mothers and newborns.
Prevalence and risk factors of Klebsiella spp. in milk samples from dairy cows with mastitis—A global systematic review
Authors: Song Jinming , Xiang Wentao , Wang Qi , Yin Jiying , Tian Tian , Yang Qizhu , Zhang Meng , Ge Guiyang , Li Jianming , Diao Naichao , Liu Fei , Shi Kun , Cai Ruopeng , Du Rui , Gong Qinglong 
Published: March 2023

IntroductionThe overall prevalence of Klebsiella spp., a group of important zoonotic pathogens, in the global dairy herds and the risk of cross-species transmission between humans and dairy cows remain to be clarified. This systematic review aimed to determine the prevalence of Klebsiella spp. in milk samples from dairy cows with mastitis worldwide and to assess the factors influencing the prevalence of these strains.MethodsQualified studies published from 2007 to 2021 were retrieved from ScienceDirect, Web of Science, PubMed, WanFang Database, China National Knowledge Infrastructure (CNKI), and VIP Chinese Journal Database. Calculations of prevalence and their 95% confidence intervals (CIs) were performed for all the studies using the Freeman-Tukey double arcsine transformation (PFT).ResultsA total of 79,852 milk samples from 55 manuscripts were examined in this meta-analysis, and 2,478 samples were found to be positive for Klebsiella spp. The pooled prevalence estimates worldwide were 7.95% (95% CI: 6.07%–10.06%), with significant heterogeneity (I2 = 98.8%, p = 0). The sampling period of 2013–2020 had a higher (p &lt; 0.05) Klebsiella-positive proportion of milk samples (12.16%, 95% CI: 8.08%–16.90%) than that of 2007–2012 (3.85%, 95% CI: 2.67%–5.21%), indicating that bovine mastitis caused by Klebsiella may become increasingly prevalent. The risk factors for the high prevalence of Klebsiella in milk samples mainly included: economic development level (developing countries; 11.76%, 95% CI: 8.25%–15.77%), mastitis type (CM; 11.99%, 95% CI: 8.62%–15.79%), and population density (&gt;500 per sq km; 10.28%, 95% CI: 2.73%–21.58%). Additionally, a bivariate meta-regression analysis revealed that the multidrug-resistance (MDR) rate of the epidemic strains was also closely related to economic development level (R2 = 78.87%) and population density (R2 = 87.51%).DiscussionDue to the potential risk of cross-species transmission between humans and cows, the prevalence of mastitis milk-derived Klebsiella and its high MDR rate need to be monitored, especially in developing countries with high population densities.
Activity of Binary Combinations of Natural Phenolics and Synthetic Food Preservatives against Food Spoilage Yeasts
Authors: Kimani Bernard Gitura , Takó Miklós , Veres Csilla , Krisch Judit , Papp Tamás , Kerekes Erika Beáta , Vágvölgyi Csaba 
Published: March 2023

Natural compounds are a suitable alternative to synthetic food preservatives due to their natural origin and health-promoting properties. In the current study, phenolic–phenolic and phenolic–synthetic combinations were tested for their antibiofilm formation, anti-planktonic growth, and anti-adhesion properties against Debaryomyces hansenii, Wickerhamomyces anomalus (formerly Pichia anomala), Schizosaccharomyces pombe, and Saccharomyces cerevisiae. The phenolics were vanillin and cinnamic acid, while the synthetic preservatives were sodium benzoate, potassium sorbate, and sodium diacetate. The vanillin–cinnamic acid combination had synergistic effect in all the tested yeasts for the biofilm inhibition with a fractional inhibitory concentration index (FICI) of ≤0.19 for W. anomalus, 0.25 for S. pombe, 0.31 for S. cerevisiae, and 0.5 for D. hansenii. Most of the phenolic–synthetic combinations had indifferent interaction regarding biofilm formation. The vanillin–cinnamic acid combination also had higher activity against spoilage yeasts adhesion on the abiotic surface and planktonic growth compared to the phenolic–synthetic combinations. For the phenolic–synthetic anti-planktonic activity, synergistic interaction was present in all the vanillin–synthetic combinations in S. pombe, vanillin–sodium benzoate and vanillin–potassium sorbate in S. cerevisiae, vanillin–sodium benzoate in W. anomalus, and cinnamic acid–sodium diacetate in S. pombe. These results suggest a novel antimicrobial strategy that may broaden the antimicrobial spectrum and reduce compound toxicity against food spoilage yeasts.
Two Complete Mitochondrial Genomes of Leptobrachium (Anura: Megophryidae: Leptobrachiinae): Characteristics, Population Divergences, and Phylogenetic Implications
Authors: Zhou Qiang , Xiang Hong-Mei , Zhang Ming-Yao , Liu Ying , Gu Zhi-Rong , Lan Xiang-Ying , Wang Jin-Xiu , Jiang Wan-Sheng 
Published: March 2023

The mustache toads Leptobrachium boringii and Leptobrachium liui are two attractive species in Megophryidae, in which adult males have mustache-like keratinized nuptial spines on their upper lip. However, both are under threat due to multiple factors, of which scientific studies are still very limited. In this study, two new complete mitochondrial genomes of L. boringii and L. liui were sequenced, assembled, and annotated based on next-generation sequencing. The mitogenome lengths of L. boringii and L. liui were found to be 17,100 and 17,501 bp, respectively, with both containing 13 protein coding genes, 23 tRNAs, 2 rRNAs, and 1 non-coding control region. Nucleotide diversity analyses indicate that atp8, atp6, and nad2 showed higher nucleotide diversity than cox1, cox3, and cytb. The intraspecific genetic distances among three different populations of L. boringii exceed 4%, and those between two populations of L. liui reach 7%. Phylogenetic relationships support their division into two subfamilies of Megophryidae (Leptobrachiinae and Megophryinae) as well as two species groups within Leptobrachium, corresponding to the number of keratinized nuptial spines (10–48 in the L. boringii species group vs. 2–6 in the L. liui species group). The two new mitogenomes reported in this study provide valuable data for future molecular evolutionary and conservation studies of the genus Leptobrachium and other Megophryidae toads.
Macrophage Migration Inhibitory Factor in Psoroptes ovis: Molecular Characterization and Potential Role in Eosinophil Accumulation of Skin in Rabbit and Its Implication in the Host–Parasite Interaction
Authors: Gu Xiaobin , Ge You , Wang Ya , Huang Cuirui , Yang Guangyou , Xie Yue , Xu Jing , He Ran , Zhong Zhijun , Yang Deying , He Zhi , Peng Xuerong 
Published: March 2023

Psoroptes ovis, a common surface-living mite of domestic and wild animals worldwide, results in huge economic losses and serious welfare issues in the animal industry. P. ovis infestation rapidly causes massive eosinophil infiltration in skin lesions, and increasing research revealed that eosinophils might play an important role in the pathogenesis of P. ovis infestation. Intradermal injection of P. ovis antigen invoked massive eosinophil infiltration, suggesting that this mite should contain some relative molecules involved in eosinophil accumulation in the skin. However, these active molecules have not yet been identified. Herein, we identified macrophage migration inhibitor factor (MIF) in P. ovis (PsoMIF) using bioinformatics and molecular biology methods. Sequence analyses revealed that PsoMIF appeared with high similarity to the topology of monomer and trimer formation with host MIF (RMSD = 0.28 angstroms and 2.826 angstroms, respectively) but with differences in tautomerase and thiol-protein oxidoreductase active sites. Reverse transcription PCR analysis (qRT-PCR) results showed that PsoMIF was expressed throughout all the developmental stages of P. ovis, particularly with the highest expression in female mites. Immunolocalization revealed that MIF protein located in the ovary and oviduct of female mites and also localized throughout the stratum spinosum, stratum granulosum, and even basal layers of the epidermis in skin lesions caused by P. ovis. rPsoMIF significantly upregulated eosinophil-related gene expression both in vitro (PBMC: CCL5, CCL11; HaCaT: IL-3, IL-4, IL-5, CCL5, CCL11) and in vivo (rabbit: IL-5, CCL5, CCL11, P-selectin, ICAM-1). Moreover, rPsoMIF could induce cutaneous eosinophil accumulation in a rabbit model and increased the vascular permeability in a mouse model. Our findings indicated that PsoMIF served as one of the key molecules contributing to skin eosinophil accumulation in P. ovis infection of rabbits.
Heterologous VvDREB2c Expression Improves Heat Tolerance in Arabidopsis by Inducing Photoprotective Responses
Authors: Zha Qian , Yin Xiangjing , Xi Xiaojun , Jiang Aili 
Published: March 2023

Extreme temperatures limit grape production and sustainability. Dehydration-responsive element-binding (DREB) transcription factors affect plant responses to temperature related stresses. Therefore, we investigated the role of VvDREB2c, a DREB-coding gene, found in grapes (Vitis vinifera L.). Protein characterization revealed that VvDREB2c is localized to the nucleus and that its AP2/ERF domain contains three β-sheets and one α-helix sheet. Analysis of the VvDREB2c promoter region revealed the presence of light-, hormone-, and stress-related cis-acting elements. Furthermore, we observed that the heterologous expression of VvDREB2c in Arabidopsis improved growth, drought tolerance, and heat tolerance. Furthermore, it improved the leaf quantum yield of regulated energy dissipation [Y(NPQ)], elevated the activities of RuBisCO, and phosphoenolpyruvate carboxylase and reduced the quantum yield of non-regulated energy dissipation [Y(NO)] in plants exposed to high temperatures. VvDREB2c-overexpressing lines also specifically upregulated several photosynthesis-related genes (CSD2, HSP21, and MYB102). In addition, VvDREB2c-overexpressing lines reduced light damage and enhanced photoprotective ability by dissipating excess light energy and converting it into heat, which eventually improves tolerance to high temperature. The contents of abscisic acid, jasmonic acid, and salicylic acid and differentially expressed genes (DEGs) in the mitogen-activated protein kinase (MAPK) signaling pathway were affected by heat stress in VvDREB2c-overexpressing lines, which indicated that VvDREB2c positively regulates heat tolerance via a hormonal pathway in Arabidopsis. VvDREB2c promotes heat tolerance in Arabidopsis by exerting effects on photosynthesis, hormones, and growth conditions. This study may provide useful insights into the enrichment of the heat-tolerance pathways in plants.
Calculation of the Heat Flux from a Ball Screw Nut to the Nut Raceway
Authors: Kuo Tzu-Chien , Yang An-Shik , Hwang Yih-Chyun , Hsieh Wen-Hsin 
Published: March 2023

In the field of precision machining, temperature fluctuation tends to cause the most significant machining errors. In particular, heat, which is generated in the nut of the ball screw feed system during movement, can deform the screw shaft significantly. In order to calculate and evaluate the thermal deformation of the ball screw shaft, the rate of the heat transfer from the nut to the screw shaft must be known. This rate can be calculated by subtracting the heat transfer rate to the nut raceway from the heat generation rate of the nut. Hence, it is necessary to calculate the heat flux from the nut to the nut raceway. This paper introduces a novel method to calculate the heat flux from the nut to the nut raceway. The new approach also enables calculations for different operating conditions. Furthermore, an experimental setup is established to measure the temperature increase, from 0 to 180 s after the nut starts moving, for various operating conditions. It is then theoretically shown that the 0–180 s temperature increase/heat flux curves for the nut are “universal”, i.e., the curve remains unchanged for the different operating conditions. Subsequently, a thermal model using the finite element method (FEM) is developed to simulate the nut temperature increase over time, which is then compared with the experimental data. As a result, it becomes possible to determine the heat flux from the nut to the nut raceway and calculate the 0–180 s temperature increase/heat flux curve (ΔT˜0~180s,Training Data) for the training group. Finally, the heat flux from the nut to the nut raceway is calculated for ten different operating conditions in the test group using the 0–180 s temperature increase/heat flux curve of the training group (ΔT˜0~180s,Training Data). The corresponding temperature curves are then calculated by inputting the values of the heat fluxes into the FEM model. The highest root mean square error (RMSE) between the calculated and experimentally measured temperature increase was 0.16 °C for Test 7 (the error was 10.7%). This result indicates that the new method is valid and feasible for calculating the heat flux from a ball screw nut to the nut raceway.
Temperature-Dependent Anisotropic Refractive Index in β-Ga2O3: Application in Interferometric Thermometers
Authors: Carrasco Daniel , Nieto-Pinero Eva , Alonso-Orts Manuel , Serna Rosalía , San Juan Jose M. , Nó María L. , Jesenovec Jani , McCloy John S. , Nogales Emilio , Méndez Bianchi 
Published: March 2023

An accurate knowledge of the optical properties of β-Ga2O3 is key to developing the full potential of this oxide for photonics applications. In particular, the dependence of these properties on temperature is still being studied. Optical micro- and nanocavities are promising for a wide range of applications. They can be created within microwires and nanowires via distributed Bragg reflectors (DBR), i.e., periodic patterns of the refractive index in dielectric materials, acting as tunable mirrors. In this work, the effect of temperature on the anisotropic refractive index of β-Ga2O3 n(λ,T) was analyzed with ellipsometry in a bulk crystal, and temperature-dependent dispersion relations were obtained, with them being fitted to Sellmeier formalism in the visible range. Micro-photoluminescence (μ-PL) spectroscopy of microcavities that developed within Cr-doped β-Ga2O3 nanowires shows the characteristic thermal shift of red–infrared Fabry–Perot optical resonances when excited with different laser powers. The origin of this shift is mainly related to the variation in the temperature of the refractive index. A comparison of these two experimental results was performed by finite-difference time-domain (FDTD) simulations, considering the exact morphology of the wires and the temperature-dependent, anisotropic refractive index. The shifts caused by temperature variations observed by μ-PL are similar, though slightly larger than those obtained with FDTD when implementing the n(λ,T) obtained with ellipsometry. The thermo-optic coefficient was calculated.
Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models
Authors: Sousa Marcela Almeida dos Santos de , Ferreira Alexia Figueiredo , da Silva Camila Caetano , Silva Marcos Andrade , Bazan Tamyris Alicely Xavier Nogueira , Monteiro Cristina de Andrade , Monteiro Andrea de Souza , Sousa Joicy Cortez de Sá , da Silva Luís Cláudio Nascimento , Zagmignan Adrielle 
Published: March 2023

This study aimed to develop a hydroxyethyl cellulose-based topical formulation containing probiotics and to evaluate its antimicrobial action using in vivo and ex vivo models. Initially, the antagonistic effects of Lacticaseibacillus rhamnosus ATCC 10863, Limosilactobacillus fermentum ATCC 23271, Lactiplantibacillus plantarum ATCC 8014 and Lactiplantibacillus plantarum LP-G18-A11 were analyzed against Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 700603, Staphylococcus aureus ATCC 27853 and Pseudomonas aeruginosa ATCC 2785. The best action was seen for L. plantarum LP-G18-A11, which presented high inhibition against S. aureus and P. aeruginosa. Then, lactobacilli strains were incorporated into hydroxyethyl cellulose-based gels (natrosol); however, only the LP-G18-A11-incorporated gels (5% and 3%) showed antimicrobial effects. The LP-G18-A11 gel (5%) maintained its antimicrobial effects and viability up to 14 and 90 days at 25 °C and 4 °C, respectively. In the ex vivo assay using porcine skin, the LP-G18-A11 gel (5%) significantly reduced the skin loads of S. aureus and P. aeruginosa after 24 h, while only P. aeruginosa was reduced after 72 h. Moreover, the LP-G18-A11 gel (5%) showed stability in the preliminary and accelerated assays. Taken together, the results show the antimicrobial potential of L. plantarum LP-G18-A11, which may be applied in the development of new dressings for the treatment of infected wounds.
Left Ventricle Detection from Cardiac Magnetic Resonance Relaxometry Images Using Visual Transformer
Authors: De Santi Lisa Anita , Meloni Antonella , Santarelli Maria Filomena , Pistoia Laura , Spasiano Anna , Casini Tommaso , Putti Maria Caterina , Cuccia Liana , Cademartiri Filippo , Positano Vincenzo 
Published: March 2023

Left Ventricle (LV) detection from Cardiac Magnetic Resonance (CMR) imaging is a fundamental step, preliminary to myocardium segmentation and characterization. This paper focuses on the application of a Visual Transformer (ViT), a novel neural network architecture, to automatically detect LV from CMR relaxometry sequences. We implemented an object detector based on the ViT model to identify LV from CMR multi-echo T2* sequences. We evaluated performances differentiated by slice location according to the American Heart Association model using 5-fold cross-validation and on an independent dataset of CMR T2*, T2, and T1 acquisitions. To the best of our knowledge, this is the first attempt to localize LV from relaxometry sequences and the first application of ViT for LV detection. We collected an Intersection over Union (IoU) index of 0.68 and a Correct Identification Rate (CIR) of blood pool centroid of 0.99, comparable with other state-of-the-art methods. IoU and CIR values were significantly lower in apical slices. No significant differences in performances were assessed on independent T2* dataset (IoU = 0.68, p = 0.405; CIR = 0.94, p = 0.066). Performances were significantly worse on the T2 and T1 independent datasets (T2: IoU = 0.62, CIR = 0.95; T1: IoU = 0.67, CIR = 0.98), but still encouraging considering the different types of acquisition. This study confirms the feasibility of the application of ViT architectures in LV detection and defines a benchmark for relaxometry imaging.
A Country's Green Brand and the Social Responsibility of Business
Authors: Ziabina Yevheniia , Dzwigol-Barosz Mariola 
Published: March 2023

Recently, the topic of carbon neutrality has been gaining momentum. Such trends are mainly related to the adoption of important global agreements, such as the European Green Deal, as well as the implementation of the Sustainable Development Goals. Such trends have a positive effect on countries, but in such situations, states must quickly respond to the challenges and needs of the world and have an understanding of how to respond to these trends. To date, there are many ratings that can be used to form a portrait of a country (investment, social, environmental, etc.), but currently the rating of the green brand of a country is becoming more relevant. There are problems regarding its improvement and filling, yet this is a new evaluation of a country for this period of time. It is relevant today to study the nature and architecture of the concept of a green brand, as well as the search for the main determinants of its impact. The purpose of the article is to analyse publications in order to identify patterns in the development of the green brand and the social responsibility of business. The article provides a bibliometric analysis of research in the field of green branding and corporate social and environmental responsibility. The authors selected more than 10,000 works published in 2000–2021 indexed by the scientometric databases Scopus, Web of Science and Google Scholar. Using VOSviewer, the results of the bibliometric analysis were visualized on the definition map. This made it possible to identify seven clusters that combine 58 terms. As a result of the research, using the constructed bibliographic map, the connection between the terms ‘green brand’ and ‘corporate social responsibility’ was revealed.
Photo-carrier induced composition separation in mixed-halide CsPb(I
x
Br1−x
)3 perovskite semiconductors; first-principles calculation
Authors: Tomita Ami , Nakayama Takashi 
Published: March 2023

Abstract
The stability of mixed-halide perovskite CsPb(I
x
Br1−x
)3 against the composition separation is studied by the first-principles calculations in the presence of photo-generated carriers. It is shown that, in the case without carriers, the mixed phase is stable around room temperature owing to the mixing-entropy effect of halide atoms. When such a mixed phase is irradiated under the light and carriers are generated, on the other hand, the composition-separated phase becomes stable. We showed that the confinement of hole carriers in I-rich regions lowers the system energy and promotes composition separation.
Komunikasi Antar Etnik di Tengah Keberagaman Budaya di Kota Kendari
Authors: Yahya Ismayanti , Bahfiarti Tuti , Farid Muhammad 
Published: March 2023

Penelitian ini bertujuan untuk mengetahui pola komunikasi antar etnis di tengah keberagaman budaya di Kecamatan Kadia, serta bentuk-bentuk komunikasi antar etnis. Teori pokok yang digunakan adalah komunikasi antar budaya dan konsep komunikasi antar etnis. Pengumpulan datanya menggunakan metode wawancara (interview) dan observasi mendalam pada informan penelitian yang dipilih secara Purposive Sampling berdasarkan tujuan penelitian. Hasil penelitian ini menghasilkan kesimpulan bahwa pola komunikasi antar etnis pada masyarakat Kecamatan Kadia Kota Kendari yang meliputi; bahasa sebagai simbol, pengalaman lintas budaya, komunikasi antar pribadi, dan pengalaman komunikasi antar etnis dengan bentuk komunikasi yang ditandai dengan berbagai hal yang lazim terjadi dalam komunikasi antar budaya seperti; komunikasi persuasif, adaptasi budaya dalam komunikasi, serta didukung oleh situasi atau lingkungan tempat mereka berinteraksi yang relatif membaur