bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mineralocorticoid receptor antagonists with sodium–glucose co-transporter-2 inhibitors in heart failure: a meta-analysis
Authors: Banerjee Mainak , Maisnam Indira , Pal Rimesh , Mukhopadhyay Satinath 
Published: August 2023

Abstract

Background and Aims
To investigate the cardiovascular effects of sodium–glucose co-transporter-2 inhibitors (SGLT2i) with concomitant mineralocorticoid receptor antagonist (MRA) use in heart failure (HF) regardless of ejection fraction (EF) and explore the risk of MRA-associated adverse events in individuals randomized to SGLT2i vs. placebo.

Methods
PubMed/MEDLINE, Web of Science, Embase, and clinical trial registries were searched for randomized controlled trials/post-hoc analyses evaluating SGLT2i in HF with or without MRA use (PROSPERO: CRD42023397129). The main outcomes were composite of first hospitalization or urgent visit for HF/cardiovascular death (HHF/CVD), HHF, and CVD. Others were all-cause mortality, composite renal and safety outcomes. Hazard ratios (HR)/risk ratios were extracted. Fixed-effects meta-analyses and subgroup analyses were performed.

Results
Five eligible studies were included, pooling data from 21 947 people with HF (type 2 diabetes mellitus, n = 10 805). Compared to placebo, randomization to SGLT2i showed a similar reduction in HHF/CVD and HHF in people who were or were not using MRAs [HHF/CVD: hazard ratio (HR) 0.75; 95% confidence interval (CI) 0.68–0.81 vs. HR 0.79; 95% CI 0.72–0.86; P-interaction = .43; HHF: HR 0.74; 95% CI 0.67–0.83 vs. HR 0.71; 95% CI 0.63–0.80; P-interaction = .53], with a suggestion of greater relative reduction in CVD in chronic HF people randomized to SGLT2i and using MRAs irrespective of EF (HR 0.81; 95% CI 0.72–0.91 vs. HR 0.98; 95% CI 0.86–1.13; P-interaction = .034). SGLT2i reduced all-cause mortality (P-interaction = .27) and adverse renal endpoints regardless of MRA use (P-interaction = .73) despite a higher risk of volume depletion with concomitant MRAs (P-interaction = .082). SGLT2i attenuated the risk of mild hyperkalaemia (P-interaction &lt; .001) and severe hyperkalaemia (P-interaction = .051) associated with MRA use.

Conclusions
MRAs did not influence SGLT2i effects on the composite of HHF/CVD, HHF or all-cause mortality; however, findings hinted at a more pronounced relative reduction in CVD in chronic HF patients regardless of EF who were randomized to SGLT2i and receiving an MRA compared to those randomized to SGLT2i and not receiving MRAs. SGLT2i attenuated the risk of MRA-associated treatment-emergent hyperkalaemia. These findings warrant further validation in well-designed randomized controlled trials.
Leishmania LPG interacts with LRR5/LRR6 of macrophage TLR4 for parasite invasion and impairs the macrophage functions
Authors: Mazumder Sayani , Sinha Archana , Ghosh Sanhita , Sharma Gurumayum Chourajit , Prusty Biswa Mohan , Manna Debasis , Pal Durba , Pal Chiranjib , Dasgupta Suman 
Published: August 2023

Abstract
Visceral leishmaniasis (VL) is a severe form of leishmaniasis, primarily affecting the poor in developing countries. Although several studies have highlighted the importance of toll-like receptors (TLRs) in the pathophysiology of leishmaniasis, the role of specific TLRs and their binding partners involved in Leishmania donovani uptake are still elusive. To investigate the mechanism of L. donovani entry inside the macrophages, we found that the parasite lipophosphoglycan (LPG) interacted with the macrophage TLR4, leading to parasite uptake without any significant alteration of macrophage cell viability. Increased parasite numbers within macrophages markedly inhibited lipopolysachharide-induced pro-inflammatory cytokines gene expression. Silencing of macrophage-TLR4, or inhibition of parasite-LPG, significantly stemmed parasite infection in macrophages. Interestingly, we observed a significant enhancement of macrophage migration, and generation of reactive oxygen species (ROS) in the parasite-infected TLR4-silenced macrophages, whereas parasite infection in TLR4-overexpressed macrophages exhibited a notable reduction of macrophage migration and ROS generation. Moreover, mutations in the leucine-rich repeats (LRRs), particularly LRR5 and LRR6, significantly prevented TLR4 interaction with LPG, thus inhibiting cellular parasite entry. All these results suggest that parasite LPG recognition by the LRR5 and LRR6 of macrophage-TLR4 facilitated parasite entry, and impaired macrophage functions. Therefore, targeting LRR5/LRR6 interactions with LPG could provide a novel option to prevent VL.
Early Divergence and Gene Exchange Highways in the Evolutionary History of Mesoaciditogales
Authors: Farrell Anne A , Nesbø Camilla L , Zhaxybayeva Olga 
Published: August 2023

Abstract
The placement of a nonhyperthermophilic order Mesoaciditogales as the earliest branching clade within the Thermotogota phylum challenges the prevailing hypothesis that the last common ancestor of Thermotogota was a hyperthermophile. Yet, given the long branch leading to the only two Mesoaciditogales described to date, the phylogenetic position of the order may be due to the long branch attraction artifact. By testing various models and applying data recoding in phylogenetic reconstructions, we observed that early branching of Mesoaciditogales within Thermotogota is strongly supported by the conserved marker genes assumed to be vertically inherited. However, based on the taxonomic content of 1,181 gene families and a phylogenetic analysis of 721 gene family trees, we also found that a substantial number of Mesoaciditogales genes are more closely related to species from the order Petrotogales. These genes contribute to coenzyme transport and metabolism, fatty acid biosynthesis, genes known to respond to heat and cold stressors, and include many genes of unknown functions. The Petrotogales comprise moderately thermophilic and mesophilic species with similar temperature tolerances to that of Mesoaciditogales. Our findings hint at extensive horizontal gene transfer (HGT) between, or parallel independent gene gains by, the two ecologically similar lineages and suggest that the exchanged genes may be important for adaptation to comparable temperature niches.
Feeling Lonely, Engaging Online During the Outbreak of COVID-19: A Longitudinal Study of Chinese Older Adults
Authors: Xu Yi , Zhou Dong 
Published: August 2023

Abstract

Background and Objectives
There is growing concern that the coronavirus disease 2019 (COVID-19) pandemic and associated social distancing policies may exacerbate mental health problems in older adults. Most studies primarily have focused on developed countries, whereas the mental consequences of the pandemic in developing countries are less well known. This study evaluates the influences of the global pandemic in relation to loneliness and family contact among older Chinese adults and examines the roles of internet adoption in alleviating the emotional experience of loneliness.

Research Design and Methods
We run individual fixed effects regression models using the 2018 and 2020 waves of the Chinese Family Panel Studies survey with 4,944 respondents aged 60 and above to capture the average within-person changes before and after the first outbreak of COVID-19 in China. We further examine the mediating effects of internet adoption on loneliness using structural equation modeling (SEM). Simultaneous SEM tested the effects of internet literacy and social network site use, and heterogeneous effects analyses were also conducted across subgroups.

Results
Fixed effects estimates indicated increased loneliness and decreased family contact during the pandemic. Moreover, more older adults were found to use the internet in 2020. The fixed-effect estimations and structural equation models consistently found that older adults’ use of the internet contributed to more social contacts and virtual meetings with their children, which have alleviated the emotional experience of loneliness.

Discussion and Implications
Digital inclusion of older adults to adopt the internet can be beneficial to improve mental well-being and alleviate loneliness during times of pandemic crisis. The targeted acceleration of digitalization to reduce the digital divide needs to be considered.
Extensive Colitis and Smoking Are Associated With Postoperative Complications Within 30 Days of Ileal Pouch–Anal Anastomosis
Authors: Barnes Edward L , Esckilsen Scott , Ashburn Jean , Barto Amy , Bloomfeld Richard , Cairns Ashley , Mehta Kurren , Patel Pooja , Dziwis Jennifer , Dunn Michael S , Rahbar Reza , Sadiq Timothy S , Hanson John S , Herfarth Hans H 
Published: August 2023

Abstract

Background
Our understanding of outcomes after proctocolectomy with ileal pouch–anal anastomosis (IPAA) for ulcerative colitis (UC) is largely based on analyses of selected populations. We created a state-level registry to evaluate the epidemiology of IPAA surgery and pouch-related outcomes across the major healthcare systems performing these surgeries in our state.

Methods
We created a retrospective cohort of all patients undergoing restorative proctocolectomy with IPAA for UC at 1 of 4 centers between January 1, 2018, and December 31, 2020. The primary outcomes of this study were the rate of complications and all-cause readmissions within the first 30 days of the final stage of IPAA surgery.

Results
During the study period, 177 patients underwent IPAA surgery with 66 (37%) experiencing a complication within 30 days. After adjusting for the number of stages in IPAA surgery, patients with extensive UC (odds ratio, 3.61; 95% confidence interval, 1.39-9.33) and current or former smokers (odds ratio, 2.98; 95% confidence interval, 1.38-6.45) were more likely to experience a complication. Among all patients, 57 (32%) required readmission within 30 days. The most common reasons for readmission were ileus/small bowel obstruction (22%), peripouch abscess (19%), and dehydration (16%).

Conclusion
In this first state-level examination of the epidemiology of IPAA for UC, we demonstrated that the complication rate after IPAA for UC was 37%, with one-third of patients being readmitted within 30 days. Extensive disease at the time of colectomy appears to be an indicator of more severe disease and may portend a worse prognosis after IPAA.
Impact of Cannabis Use on Immune Cell Populations and the Viral Reservoir in People With HIV on Suppressive Antiretroviral Therapy
Authors: Falcinelli Shane D , Cooper-Volkheimer Alicia D , Semenova Lesia , Wu Ethan , Richardson Alexander , Ashokkumar Manickam , Margolis David M , Archin Nancie M , Rudin Cynthia D , Murdoch David , Browne Edward P 
Published: August 2023

Abstract

Background
Human immunodeficiency virus (HIV) infection remains incurable due to the persistence of a viral reservoir despite antiretroviral therapy (ART). Cannabis (CB) use is prevalent amongst people with HIV (PWH), but the impact of CB on the latent HIV reservoir has not been investigated.

Methods
Peripheral blood cells from a cohort of PWH who use CB and a matched cohort of PWH who do not use CB on ART were evaluated for expression of maturation/activation markers, HIV-specific T-cell responses, and intact proviral DNA.

Results
CB use was associated with increased abundance of naive T cells, reduced effector T cells, and reduced expression of activation markers. CB use was also associated with reduced levels of exhausted and senescent T cells compared to nonusing controls. HIV-specific T-cell responses were unaffected by CB use. CB use was not associated with intact or total HIV DNA frequency in CD4 T cells.

Conclusions
This analysis is consistent with the hypothesis that CB use reduces activation, exhaustion, and senescence in the T cells of PWH, and does not impair HIV-specific CD8 T-cell responses. Longitudinal and interventional studies with evaluation of CB exposure are needed to fully evaluate the impact of CB use on the HIV reservoir.
A scoping review of clinical handover mnemonic devices
Authors: Yung Amos H W , Pak Chi Shing , Watson Bernadette 
Published: August 2023

Abstract
Since the Institute of Medicine (IOM) published To Err is Human: Building a Safer Health System in 1999, clinical handovers (or handoffs) and their relationship with the communication of patient safety have raised concerns from the public, regulatory bodies, and medical practitioners. Protocols, guidelines, forms, and mnemonic devices have been created to ensure safer clinical handovers. An initial literature search did not find a framework to describe the clinical processes and functions of each mnemonic device and its elements. The absence of a systematic framework could hinder the study across and the reusability of the established clinical handover mnemonic devices. This study aims to develop a universal framework to describe the clinical processes and functions essential for patient safety during handover. We queried PubMed.gov and obtained 98 articles related to clinical handovers. We examined the citing sources of the mnemonics mentioned in these articles. A total of 42 handover mnemonics with 238 elements were identified. Our review noted that there was no taxonomy to describe the clinical functions and process associated with the clinical handover mnemonic devices. We used grounded theory to address this gap and built a new taxonomy from the 42 mnemonics. A researcher read all mnemonics, developed a taxonomy for tagging clinical handover mnemonics, and categorized all mnemonic elements into correct processes and functions. After that, the second researcher, a medical practitioner, examined the taxonomy and made suggested corrections for the labelled functions of all mnemonic elements. Both researchers agreed on the taxonomy and the labelled processes and functions of different mnemonic elements. The taxonomy contains three processes and twenty functions in clinical handovers. Clinical processes like ‘medical condition’, ‘medical history’, ‘medical evaluation’, ‘care plan’, ‘outstanding care/tasks/results’, and ‘patient information’, as an administrative process, were widely adopted in clinical handover mnemonics. Moreover, mnemonic elements on communication manner and information validation had been identified in the list of clinical handover mnemonics. Although we recognize challenges because of both the vast number of clinical handover scenarios and the task of placing them under a few predefined groups, our findings suggest that such a taxonomy, as developed for this study, could assist medical practitioners to devise a clinical handover mnemonic to best fit their workplace.
Smart Multivariate Spectrophotometric Determination of Two Co-Administered Autoimmune Drugs; Sulfasalazine and Pentoxifylline; in Bulk and Spiked Human Plasma
Authors: Magdy Maimana A , Farid Nehal F , Anwar Basma H , Abdelhamid Nessreen S 
Published: August 2023

Abstract

Background
Sulfasalazine and pentoxifylline are co-prescribed together to treat psoriasis and pemphigus vulgaris. Sulfasalazine is an anti-inflammatory, immunosuppressant, and antibiotic drug, while pentoxifylline is a vasodilator and immunosuppressant. The spectra of the two drugs and plasma suffer from severe overlap.

Objective
This work aims to simultaneously determine sulfasalazine and pentoxifylline in their binary mixture and spiked human plasma by the assessment of their UV spectral data.

Methods
Two model updated chemometric methods were established using principal component regression and partial least-squares regression models. The two models were validated in accordance with the U.S. Food and Drug Administration guidelines for bioanalysis and were applied for the determination of both drugs in synthetic mixtures or spiked human plasma.

Results
Accuracy and precision were within the accepted limits. In addition, three different assessment methods were used to evaluate the environmental greenness of the proposed models.

Conclusion
The two updated models are simple, rapid, sensitive, and precise, and could be easily applied in QC laboratories for determination of sulfasalazine and pentoxifylline, without any preliminary separation steps or interference from plasma matrix.

Highlights
Two updated chemometric models called principlal component regression and partial least-squares regression were established for determination of sulfasalazine and pentoxifylline in spiked human plasma using UV spectrophotometric data.
Organic zinc glycine chelate is better than inorganic zinc in improving growth performance of cherry valley ducks by regulating intestinal morphology, barrier function, and the gut microbiome
Authors: Chang Yaqi , Wang Ke , Wen Min , Wu Bing , Liu Guangmang , Zhao Hua , Chen Xiaoling , Cai Jingyi , Jia Gang 
Published: August 2023

Abstract
Zinc (Zn) is an essential trace element that has physiological and nutritional functions. However, excessive use of Zn can lead to waste of resources. In this study, we compared the effects of inorganic (ZnSO4) and organic Zn glycine chelate (Zn-Gly) on the growth performance, intestinal morphology, immune function, barrier integrity, and gut microbiome of Cherry Valley ducks. We randomly divided 180 one-day-old male meat ducks into three groups, each with six replicates of 10 birds: basal diet group (CON), basal diet with 70 mg Zn/kg from ZnSO4 (ZnSO4 group), and basal diet with 70 mg Zn/kg from Zn-Gly (Zn-Gly group). After 14 and 35 d of feeding, birds in the Zn groups had significantly increased body weight and average daily gain (ADG), decreased intestinal permeability indicator d-lactate, improved intestinal morphology and barrier function-related tight junction protein levels, and upregulated mucin 2 and secretory immunoglobulin A levels compared to the control (P &lt; 0.05). Additionally, compared to the ZnSO4 group, we found that supplementation with Zn-Gly at 70 mg/kg Zn resulted in the significant increase of body weight at 35 d, 1 to 35 d ADG and average daily feed intake, villus height at 14 and 35 d, secretory immunoglobulin A and immunoglobulin G at 14 d, and mucin 2 mRNA level at 14 d (P &lt; 0.05). Compared with the control group, dietary Zn had a significant effect on the gene expression of metallothionein at 14 and 35 d (P &lt; 0.05). 16S rRNA sequencing showed that Zn significantly increased alpha diversity (P &lt; 0.05), whereas no differences in beta diversity were observed among groups (P &gt; 0.05). Dietary Zn significantly altered the cecal microbiota composition by increasing the abundances of Firmicutes, Blautia, Lactobacillus, Prevotellaceae NK3B31, and [Ruminococcus] torques group and reducing that of Bacteroides (P &lt; 0.05). Spearman correlation analysis revealed that the changes in microbiota were highly correlated (P &lt; 0.05) with growth performance, intestinal morphology, and immune function-related parameters. Taken together, our data show that, under the condition of adding 70 mg/kg Zn, supplementation with Zn-Gly promoted growth performance by regulating intestinal morphology, immune function, barrier integrity, and gut microbiota of Cherry Valley ducks compared with the use of ZnSO4 in feed.
A new method in reservoir rock classification in carbonate and sandstone formations
Authors: Omrani Hashem , Hajipour Mastaneh , Jamshidi Saeid , Behnood Mohammad 
Published: August 2023

AbstractThis study aims to improve rock-type classification by analyzing core data based on the water–oil primary drainage capillary pressure method. A modified empirical equation is proposed using permeability, porosity, and irreducible water saturation to classify rock types based on water–oil primary drainage capillary pressure. We used primary drainage capillary pressure data measured in carbonate and sandstone samples in the Ahvaz Asmari and Mansouri oilfields to evaluate the characterization number (Cn) method. This study consists of two main parts. First, the Cn method is introduced to rock typing, and the permeability is calculated from well log data. In the second part, we present rock-type classification when the water saturation of the formation is more than the irreducible water saturation. The novelty of this work is a simple and efficient technique to rock-type classification using the Cn method. In addition, we present a procedure to assign rock types for the transition zone using the Cn method. Moreover, this study systematically investigates the role of irreducible water saturation in rock typing. The innovation of this work lies in its ability to classify rocks in heterogeneous reservoirs for carbonate and sandstone lithology and allow for the calculation of permeability more accurately from well log data. The comparison results between the Cn method and the flow zone indicator method show the robust clustering ability of the Cn method.
Proximal femoral derotation osteotomy for management of femoral malversion: a systematic review
Authors: Sohatee Mark , Jaibaji Monketh , Malviya Ajay 
Published: August 2023

ABSTRACT
Femoral malversion is an under-recognized contributor to hip pain in younger adults. Under treatment is often a contributor to poor outcomes in hip preservation surgery. We reviewed the literature to analyse the outcomes of proximal femoral derotation osteotomy as a treatment for femoral malversion as well as propose our own management algorithm for treating such patients.
A systematic review was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines searching four databases (PubMed, CINALH, MEDLINE and EMBASE) for studies investigating the outcomes of derotation osteotomy in treating malversion. Nine studies were found encompassing 229 hips.
At a mean follow-up of 39.9 months across the studies, there were only two conversions (1%) to total hip arthroplasty and four revision cases in total. Seven of the nine studies reported improved functional outcomes in their cohorts, with the mean Harris hip score improved from 63.7 to 87.3 where reported.
There is a paucity of literature around the outcomes of proximal femoral derotation osteotomy. However, both the evidence available and the authors’ experience suggest that consideration of femoral malversion is an essential component of hip preservation surgery, improving functional outcomes in cases of excessive femoral anteversion and femoral retroversion.