bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reducing the effects of greenhouse gas emissions of organic waste by utilizing banana stems as starter local pig feed
Authors: Ullo N Z A , Yoku O , Monim H , Widayati T W , Kayadoe M 
Published: June 2023

Abstract
In Tanah Papua, banana stems have been used as feed by local pig farmers but with a very simple processing method so that has not had a significant impact on the economic traits local pig production. The research aim is to determine an appropriate level of FBSs as a complete feed for local pig’s starters. A total of 12 local piglets aged about 6-12 weeks were given feed treatment using FBSs at levels of 0, 5,10 and 15%. The variables studied were the level of ration consumption (dry matter consumption, crude protein consumption, crude fiber consumption), daily again, and feed efficiency. The data were analyzed using ANOVA with SPSS version 24 program, then the results of ANOVA gave a significant effect followed by the Tukey test. The results showed that the complete feed treatment of FBS was quite good for local pigs starter because the pigs showed good performance. The FBS complete feed produced crude protein (CP) and metabolic energy (ME) relatively higher than the recommended Indonesian national standard (SNI) for pig starter feed. Feed treatment at 10% FBS was considered quite good based on the average daily gain (ADG) and feed efficiency (FE).
The acclimatization compartment and growth hormone impact on the life percentage of endangered Hopea gregaria tree species.
Authors: Tuheteru F D , Arif A , Husna  , Albasri  , Andriyani S , Prameswari D 
Published: June 2023

Abstract
In Southeast Sulawesi province, Indonesia there is an endemic tree species with the scientific name of Hopea gregaria Slooten belonging to Dipterocarpaceae family. The wildings of this species, named pooti locally, were available abundantly at Nipa-Nipa Grand Forest Park. These wildings may be collected for plant procurement programs. Unsustainable forest exploitation management in the past and also forest fire, have caused this species is classified as an endangered forest tree species in Indonesia and this tree species really needs to be sustained. This study was carried out at the glasshouse of the Association of Indonesian Mycorrhizae in the Southeast Sulawesi branch for three months. A factorial completely randomized design was used, consisting of 2 factors namely provision of acclimatization compartment, without and with cover, and Rootone-F concentration of 0, 50, 100, and 150 ppm. An analysis of variance (F-test) was used, if the result was significant, further analyses using Duncan Multiple Range Test will be done. Results revealed that the acclimatization compartment and Rootone-F interaction had no effect on all variables. However, acclimatization can increase the life percentage of shoots, wildings’ height, and number and leaf length. In the beginning, natural wildings rely on acclimatization while Rootone-F increases wildings’ height.
iEnhance: a multi-scale spatial projection encoding network for enhancing chromatin interaction data resolution
Authors: Li Kai , Zhang Ping , Wang Zilin , Shen Wei , Sun Weicheng , Xu Jinsheng , Wen Zi , Li Li 
Published: June 2023

Abstract
Although sequencing-based high-throughput chromatin interaction data are widely used to uncover genome-wide three-dimensional chromatin architecture, their sparseness and high signal-noise-ratio greatly restrict the precision of the obtained structural elements. To improve data quality, we here present iEnhance (chromatin interaction data resolution enhancement), a multi-scale spatial projection and encoding network, to predict high-resolution chromatin interaction matrices from low-resolution and noisy input data. Specifically, iEnhance projects the input data into matrix spaces to extract multi-scale global and local feature sets, then hierarchically fused these features by attention mechanism. After that, dense channel encoding and residual channel decoding are used to effectively infer robust chromatin interaction maps. iEnhance outperforms state-of-the-art Hi-C resolution enhancement tools in both visual and quantitative evaluation. Comprehensive analysis shows that unlike other tools, iEnhance can recover both short-range structural elements and long-range interaction patterns precisely. More importantly, iEnhance can be transferred to data enhancement of other tissues or cell lines of unknown resolution. Furthermore, iEnhance performs robustly in enhancement of diverse chromatin interaction data including those from single-cell Hi-C and Micro-C experiments.
O005 Evaluation of occludin, a tight junction transmembrane protein in human lung cancer and its potential role in metastatic disease
Authors: Poobalan N , Jiang WG , Martin TA 
Published: June 2023

Abstract

Introduction
Tight junctions (TJs) maintain cell adhesion. Modulation can promote cell dissociation/motility/ metastasis. Occludin is a transmembrane protein within TJs and may play a role in TJ modulation. The aim of this research was to identify the expression of Occludin in human lung cancer and its effect in cell motility/dissociation.

Methods
qPCR was used to quantify Occludin transcripts in a human lung cancer cohort (n=80) (Peking University Cancer Hospital Research Ethics Committee). In vitro experiments on A549/COR-L23 lung cancer cell lines included immunofluorescence staining, cell attachment/migration using ECIS Z-Theta. Cells were treated with Hepatocyte Growth Factor (HGF, 50ng/μl) and/or cell migration inhibitors N-WASPi (Wiskostatin) and ROCKi (Y-27632).

Results
qPCR analysis revealed reduced expression of Occludin in patients with metastasis (no bone mets 21.7+/- 12 v bone mets 3.23+/- 3 (p=0.42) v boneActv 0.263+/-0.096 (p=0.077). A549 cells showed a similar cell migration pattern with HGF, ROCKi/HGF+ROCKi. In COR-L23, HGF increased cell migration; ROCKi/HGF+ROCKi showed suppression. N-WASPi/HGF+N-WASPi prevented all cell migration. Immunofluorescence demonstrated that HGF caused loss of Occludin from cell-to-cell membranes with single cells and increased pseudopodia in COR-L23 and A549.

Conclusion
This preliminary study suggests an association between Occludin and bone metastasis in lung cancer. HGF promoted Occludin loss from cell membranes, cell dissociation and increased pseudopodia/increased cell motility. This suggests that loss of Occludin from cell membranes is linked to reduced TJ function leading to cell dissociation/increased motility. NWASPi may provide pharmacological value in future lung carcinoma treatment.
O010 The impact of intravenous iron on physical activity levels and quality of life in advanced cancer: results from the ICARUS randomised controlled trial
Authors: Dickson EA , Ng O , Keeler B , Wilcock A , Brookes M , Acheson AG 
Published: June 2023

Abstract

Introduction
Anaemia and fatigue are highly prevalent in advanced cancer. Intravenous iron (IVI) has shown promise as an effective treatment for anaemia across a number of conditions. For the first time, we explore the impact of IVI on physical activity and quality of life (QoL) in advanced, inoperable cancer.

Methods
We randomised anaemic, fatigued patients with advanced solid cancers to receive either IVI (iron isomaltoside) or placebo (0.9% NaCl) in a double-blind design. Activity was assessed using a pedometer (Fitbit Flex 2) for 8 days pre- infusion and at 4 and 8 weeks post-infusion. The minimum clinically important difference (MCID) was defined as 600 daily steps. Three validated QoL questionnaires were completed at the same timepoints.

Results
34 patients were recruited over 15 months (17 IVI, 17 placebo). Haemoglobin was higher with IVI at 4 weeks (MD 7.3g/L, P=0.047) and 8 weeks (MD 4.3g/L, P=0.13). At 8 weeks the mean difference in daily steps was 1707 (1142) steps (IVI +318 [1195] from baseline; placebo -1658 [2846]). On QoL measures global health (MD 15.8, P=0.027) and social functioning (MD 14.9, P=0.019) improved with IVI at 4 weeks. Fatigue scores exceeded the intergroup MCID for IVI at both follow up timepoints (week 4 MD 3.38 [-14.94–8.18], P=0.548], week 8 MD 3.49 [-17.52–10.53], P=0.609).

Conclusion
IVI maintained physical activity and improved fatigue specific QoL. Placebo was associated with a significant decline activity and fatigue specific QoL. IVI may delay the functional decline experienced with advanced cancer.

Take-home message
Intravenous iron led to clinically meaningful improvements in quality of life and physical activity in anaemic patients with advanced cancer.
TU1.4 Adequacy of endoscopic photo-documentation in an OGD: Closing the loop!
Authors: Fatima Iayla , Sadoon Adham , Mehmood Umer 
Published: June 2023

Abstract

Aims
The technical competence of Oesophago-Gastro-Duodenoscopy (OGD) is rapidly acquired but to support the practice of complete examination, the British Society of Gastroenterology (BSG) and Association of Gastrointestinal Surgeons of Great Britain and Ireland (AUGIS) recommend documentation of 8 anatomical stations. Photo-documentation ensures a complete examination, gives an opportunity to inspect area of interest and serves as legal record of an adequate procedure. Our Aim was to assess the hospitals practice of photo-documentation against the BSG and AUGIS guidelines and to improve practice by conducting teaching sessions and designing a poster for the endoscopy rooms.

Methods
1st Cycle: 100 OGD reports were randomly selected from 1/1/2018 to 31/3/2020 and were reviewed on the hospitals electronic reporting system (Endorad). Data on patients demographics, speciality of Endoscopist and the documented stations was collected. Data was presented at the departments meeting and a simultaneous teaching session was conducted. A poster was designed and displayed outside the endoscopy rooms.
2nd Cycle: 100 OGD reports were selected from 1st January to 30th June 2021 and re audited.

Results
66% of the OGD reports had photo-documentation of all 8 stations and 81% of reports had 7 documented stations which was a significant improvement in practice as the first audit had no report with photographic documentation of all the stations. The highest number of stations documented in the first audit were 5 and that too in only 40% of the cases.

Conclusions
The audit cycle showed significant improvement in photo-documentation of all the stations, highlighting the benefit of the process.
What is central to renal nutrition: protein or sodium intake?
Authors: Wang Angela Yee-Moon , Mallamaci Francesca , Zoccali Carmine 
Published: June 2023

ABSTRACT
Historically, nutrition intervention has been primarily focused on limiting kidney injury, reducing generation of uraemic metabolites, as well as maintaining nutrition status and preventing protein-energy wasting in patients with chronic kidney disease (CKD). This forms an important rationale for prescribing restricted protein diet and restricted salt diet in patients with CKD. However, evidence supporting a specific protein intake threshold or salt intake threshold remains far from compelling. Some international or national guidelines organizations have provided strong or ‘level 1’ recommendations for restricted protein diet and restricted salt diet in CKD. However, it is uncertain whether salt or protein restriction plays a more central role in renal nutrition management. A key challenge in successful implementation or wide acceptance of a restricted protein diet and a restricted salt diet is patients’ long-term dietary adherence. These challenges also explain the practical difficulties in conducting randomized trials that evaluate the impact of dietary therapy on patients’ outcomes. It is increasingly recognized that successful implementation of a restricted dietary prescription or nutrition intervention requires a highly personalized, holistic care approach with support and input from a dedicated multidisciplinary team that provides regular support, counselling and close monitoring of patients. With the advent of novel drug therapies for CKD management such as sodium-glucose cotransporter-2 inhibitors or non-steroidal mineralocorticoid receptor antagonist, it is uncertain whether restricted protein diet and restricted salt diet may still be necessary and have incremental benefits. Powered randomized controlled trials with novel design are clearly indicated to inform clinical practice on recommended dietary protein and salt intake threshold for CKD in this new era.
Bow hunter syndrome due to severe stenosis of the left subclavian artery with a thoracic aortic aneurysm
Authors: Komaki Hisaaki , Minatoguchi Shinya 
Published: June 2023

Abstract
Bow hunter syndrome is a rare condition characterized by repetitive syncope, which is reflected in brain stem ischaemia due to vertebral compression induced by head rotation. We herein report a rare presentation of bow hunter syndrome due to severe stenosis of the left subclavian artery with a thoracic aortic aneurysm. Disease, hypoplasia of the vertebral artery, or degenerative bone disease as well as congenital foraminal narrowing can be causative. However, to our knowledge, this is the first report describing bow hunter syndrome due to severe stenosis of the left subclavian artery. Computed tomography angiography performed with the symptom induced by head rotation demonstrated obstruction of vertebral arteries. The patient underwent successful open-chest surgery and subclavian artery construction, and postural dizziness was resolved. This report may be helpful when treating similar cases.
smalldisco, a pipeline for siRNA discovery and 3′ tail identification
Authors: Caldas Ian V , Kelley Leanne H , Ahmed-Braimah Yasir H , Maine Eleanor M 
Published: June 2023

Abstract
Capturing and sequencing small RNAs is standard practice; however, identification of a group of these small RNAs—small interfering RNAs (siRNAs)—has been more difficult. We present smalldisco, a command-line tool for small interfering RNA discovery and annotation from small RNA-seq datasets. smalldisco can distinguish short reads that map antisense to an annotated genomic feature (e.g. exons or mRNAs), annotate these siRNAs, and quantify their abundance. smalldisco also uses the program Tailor to quantify 3′ nontemplated nucleotides of siRNAs or any small RNA species. smalldisco and supporting documentation are available for download from GitHub (https://github.com/ianvcaldas/smalldisco) and archived in Zenodo (https://doi.org/10.5281/zenodo.7799621).
A comprehensive in vivo screen of yeast farnesyltransferase activity reveals broad reactivity across a majority of CXXX sequences
Authors: Kim June H , Hildebrandt Emily R , Sarkar Anushka , Yeung Wayland , Waldon La Ryel A , Kannan Natarajan , Schmidt Walter K 
Published: June 2023

Abstract
The current understanding of farnesyltransferase (FTase) specificity was pioneered through investigations of reporters like Ras and Ras-related proteins that possess a C-terminal CaaX motif that consists of 4 amino acid residues: cysteine–aliphatic1–aliphatic2–variable (X). These studies led to the finding that proteins with the CaaX motif are subject to a 3-step post-translational modification pathway involving farnesylation, proteolysis, and carboxylmethylation. Emerging evidence indicates, however, that FTase can farnesylate sequences outside the CaaX motif and that these sequences do not undergo the canonical 3-step pathway. In this work, we report a comprehensive evaluation of all possible CXXX sequences as FTase targets using the reporter Ydj1, an Hsp40 chaperone that only requires farnesylation for its activity. Our genetic and high-throughput sequencing approach reveals an unprecedented profile of sequences that yeast FTase can recognize in vivo, which effectively expands the potential target space of FTase within the yeast proteome. We also document that yeast FTase specificity is majorly influenced by restrictive amino acids at a2 and X positions as opposed to the resemblance of CaaX motif as previously regarded. This first complete evaluation of CXXX space expands the complexity of protein isoprenylation and marks a key step forward in understanding the potential scope of targets for this isoprenylation pathway.
Changes in the expression pattern of OsWUS negatively regulate plant stature and panicle development in rice
Authors: Tang Huiwu , Fan Jinlan , Wang Ru , Zhu Jiahui , Xiang Xinzhu , Dong Jingfang , Zhou Lingyan , Wang Limin 
Published: June 2023

Abstract
WUSCHEL (WUS) and WUSCHEL-RELATED HOMEOBOX (WOX) encode transcription factors and play important roles in regulating the formation and maintenance of shoot and floral meristems. OsWUS have distinct functions in meristem development with slightly tuned expression. However, the mechanisms regulating the specific expression of OsWUS need to be further explored. In this study, an abnormal expression mutant of OsWUS, called Dwarf and aberrant panicle 1 (Dap1) was used. In order to identify the causal gene in Dap1, high-efficiency thermal asymmetric interlaced (hiTAIL)-PCR and co-segregation analysis were performed. We surveyed the growth and yield traits in Dap1 and wild type. Changes in gene expression between Dap1 and wild type were determined by RNA-seq. The Dap1 mutant is due to the T-DNA inserted at 3,628-bp upstream of the translation start codon of OsWUS. Plant height, tiller numbers, panicle length, the number of grains per main panicle, and the number of secondary branches was significantly reduced in the Dap1 mutant. The expression of OsWUS was markedly increased in Dap1 mutant plants compared to the wild type, which might be due to a disruption in the genomic sequence integrity. Simultaneously, the expression levels of gibberellic acid-related genes and genes involved in panicle development were significantly changed in the Dap1 mutant. Our results suggest that OsWUS is a precise regulatory element, its specific spatio-temporal expression pattern is critical for its function, and both loss-of-function and gain-of-function mutations lead to abnormal plant growth.
Evaluation of MIC distribution and establishment of epidemiological cut-off values for Streptococcus parauberis isolated from aquatic animals
Authors: Woo Soo Ji , Kim Myoung Sug , Do Mi Young , Kim Na Young , Joo Min Soo 
Published: June 2023

Abstract

Aims
Streptococcus parauberis is responsible for the development of streptococcosis in marine fish. The aim of the current study was to determine the antimicrobial susceptibility of aquatic Strep. parauberis strains, thus establishing laboratory-specific epidemiological cut-off (COWT) values to distinguish wild-type (WT) and nonwild-type (NWT) strains.

Methods and results
Using 220 Strep. parauberis isolates obtained from diseased Paralichthys olivaceus, Platichthys stellatus, and Sebastes schlegelii over 6 years from seven different locations in Korea, we established COWT values for eight common antimicrobial agents using the standard broth microdilution method. The COWT values calculated using MIC distribution with the NRI and ECOFFinder methods were the same or within one dilution step for the eight antimicrobials tested. Nine NWT isolates with decreased susceptibility to at least two antimicrobials and one of these isolates exhibited decreased susceptibility to six antimicrobial agents were identified using COWT values based on NRI.

Conclusions
Interpretive criteria for Strep. parauberis have not yet been established, and the findings of this study provide putative COWT values for eight antimicrobial agents frequently used in aquaculture in Korea.
Building relational confidence in remote and hybrid work arrangements: novel ways to use digital technologies to foster knowledge sharing
Authors: Keppler Samantha M , Leonardi Paul M 
Published: June 2023

Abstract
Remote and hybrid workers know fewer of their colleagues and have fewer strong workplace relationships. If strong relationships support knowledge sharing, workers will have a harder time getting knowledge they need. Prior research shows that digital communication technologies increase workers’ network-level knowledge of “who knows what” and “who knows who.” Yet, knowledge seekers may be hesitant to ask for knowledge, particularly when they have concerns that their relationship with a knowledge source is too distant. We conduct a dyad-level study of 141 instances of knowledge seeking among employees of a South American telecommunications company employing a hybrid work arrangement and using an enterprise social media called Chatter. We find that specific uses of the technology help develop what we call “relational confidence,” or the confidence that one has a close enough relationship to a colleague to ask and get needed knowledge. With greater relational confidence, knowledge sharing is more successful.
The hematopoietic microenvironment: a network of niches for the development of all blood cell lineages
Authors: Hernández-Barrientos Daniel , Pelayo Rosana , Mayani Hector 
Published: June 2023

AbstractBlood cell formation (hematopoiesis) takes place mainly in the bone marrow, within the hematopoietic microenvironment, composed of a number of different cell types and their molecular products that together shape spatially organized and highly specialized microstructures called hematopoietic niches. From the earliest developmental stages and throughout the myeloid and lymphoid lineage differentiation pathways, hematopoietic niches play a crucial role in the preservation of cellular integrity and the regulation of proliferation and differentiation rates. Current evidence suggests that each blood cell lineage develops under specific, discrete niches that support committed progenitor and precursor cells and potentially cooperate with transcriptional programs determining the gradual lineage commitment and specification. This review aims to discuss recent advances on the cellular identity and structural organization of lymphoid, granulocytic, monocytic, megakaryocytic, and erythroid niches throughout the hematopoietic microenvironment and the mechanisms by which they interconnect and regulate viability, maintenance, maturation, and function of the developing blood cells.
Article 17.6(ii) of the WTO Anti-Dumping Agreement: Waiting for Chekhov’s Gun to Go Off
Authors: Rovnov Yury 
Published: June 2023

Abstract
Article 17.6(ii) of the WTO Anti-Dumping Agreement is a one-of-a-kind treaty norm that sets out a deferential standard of review for issues of law by requiring panels to accept any ‘permissible’ interpretation. The Appellate Body’s approach to analysis under Article 17.6(ii), which precluded a finding of two competing interpretations being permissible, has long remained a point of criticism by the USA, a frequent respondent in anti-dumping disputes. In the wake of the first arbitration award under the Multi-Party Interim Appeal Arbitration Aarrangement (MPIA), which seeks to give more effect to the deferential standard of review, this article emphasizes the unprecedented character of Article 17.6(ii) in international law, argues that expectations of how it would operate arose from a misapprehension of Chevron deference in US law and that the whole issue may be another reason for having a separate dispute settlement track for trade remedies.
Tunneled Trial of Spinal Cord Stimulation
Authors: Pritzlaff Scott G. , Kreis Paul G. , Fishman Scott M. 
Published: June 2023

Abstract
This chapter explores the tunneled trial of spinal cord stimulation (SCS). It highlights the importance of the trial being performed in an operating room (OR). Tunneled trials are often the preferred approach for post-laminectomy patients with extensive fusion who are not candidates for traditional percutaneous trials. Surgeons often perform a laminotomy and place a paddle lead in the dorsal epidural space along the anatomic midline. Then, the midline incision and pocket can be created before the placement of leads. The chapter also discusses the conditions, advantages, and disadvantages of placing one or two leads. It recognizes lead migration as the most common cause of mechanical SCS failure.