bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nurse‐led web‐based patient education reduces anxiety in thyroidectomy patients: A randomized controlled study
Authors: Altinbas Bahar Candas , Gürsoy Ayla 
Published: January 2023

AbstractAimThe aim of this study was to determine the effect of web‐based patient education on anxiety in patients scheduled for thyroid surgery. A secondary aim was to evaluate the patients' postoperative recovery outcomes.DesignThis study is a randomized controlled trial.MethodThe study was conducted at a university hospital in Turkey between September 2018 and May 2019 with 76 patients scheduled for thyroidectomy surgery and randomly assigned into two groups. Patients in the intervention group received web‐based education, while those in the control group were given only routine care. Measurements were performed before surgery, on the day of surgery, and 1 week after discharge.ResultsOn the day of surgery, intervention group anxiety levels were lower than those of the control group, and the majority of early recovery parameters were significantly better in the intervention group than in the control group. One week after discharge, the intervention group patients' anxiety levels were significantly lower. Web‐based education had no impact on pain, time in the operating room, readmission to the hospital after discharge, or length of hospitalization.ConclusionsNurse‐led web‐based education reduced patients' anxiety regarding surgery. The results also show that it may improve postoperative early recovery.
Inherited deletion of 9p22.3‐p24.3 and duplication of 18p11.31‐p11.32 associated with neurodevelopmental delay: Phenotypic matching of involved genes
Authors: Ajami Naser , Kerachian Mohammad Amin , Toosi Mehran Beiraghi , Ashrafzadeh Farah , Hosseini Susan , Robinson Peter N. , Abbaszadegan Mohammad Reza 
Published: January 2023

AbstractWe describe a 3.5‐year‐old Iranian female child and her affected 10‐month‐old brother with a maternally inherited derivative chromosome 9 [der(9)]. The postnatally detected rearrangement was finely characterized by aCGH analysis, which revealed a 15.056 Mb deletion of 9p22.3‐p24.3p22.3 encompassing 14 OMIM morbid genes such as DOCK8, KANK1, DMRT1 and SMARCA2, and a gain of 3.309 Mb on 18p11.31‐p11.32 encompassing USP14, THOC1, COLEC12, SMCHD1 and LPIN2. We aligned the genes affected by detected CNVs to clinical and functional phenotypic features using PhenogramViz. In this regard, the patient's phenotype and CNVs data were entered into PhenogramViz. For the 9p deletion CNV, 53 affected genes were identified and 17 of them were matched to 24 HPO terms describing the patient's phenotypes. Also, for CNV of 18p duplication, 22 affected genes were identified and six of them were matched to 13 phenotypes. Moreover, we used DECIPHER for in‐depth characterization of involved genes in detected CNVs and also comparison of patient phenotypes with 9p and 18p genomic imbalances. Based on our filtration strategy, in the 9p22.3‐p24.3 region, approximately 80 pathogenic/likely pathogenic/uncertain overlapping CNVs were in DECIPHER. The size of these CNVs ranged from 12.01 kb to 18.45 Mb and 52 CNVs were smaller than 1 Mb in size affecting 10 OMIM morbid genes. The 18p11.31‐p11.32 region overlapped 19 CNVs in the DECIPHER database with the size ranging from 23.42 kb to 1.82 Mb. These CNVs affect eight haploinsufficient genes.
Sinomenine alleviates diabetic peripheral neuropathic pain through inhibition of the inositol‐requiring enzyme 1 alpha–X‐box binding protein 1 pathway by downregulating prostaglandin‐endoperoxide synthase 2
Authors: Chen Ji , Guo Peng , Liu Xinxin , Liao Huizhi , Chen Kemin , Wang Yuxia , Qin Jie , Yang Fengrui 
Published: January 2023

AbstractIntroductionWe tried to show the effect of sinomenine (SIN) in diabetic peripheral neuropathic pain (DPNP) and the related underlying mechanism.MethodsNetwork pharmacological analysis and bioinformatics analysis were carried out for identification of the active ingredients ofSinomenium acutumand the related genes. The DPNP rat model was constructed and primary rat spinal cord microglial cells were isolated forin vitrocell experiments. The therapeutic role of SIN in DPNP was determinedin vivoandin vitrothrough analysis of microglial cell activation and inflammatory response.ResultsTherapeutic role ofS. acutumin DPNP was mainly achieved by regulating 14 key genes, among which the target gene prostaglandin‐endoperoxide synthase 2 (PTGS2) of SIN might be the key gene.Anin vivoexperiment showed that SIN inactivated the inositol‐requiring enzyme 1 alpha–X‐box binding protein 1 pathway by downregulatingPTGS2, which relieved pain symptoms in DPNP rats. It was confirmedin vivothat SIN inhibited the pathway throughPTGS2to alleviate the activation of spinal cord microglial cells and inflammatory response.ConclusionSIN decreases the expression ofPTGS2to inactivate the inositol‐requiring enzyme 1 alpha–X‐box binding protein 1 signaling pathway, which inhibits microglial activation, as well as the release of inflammatory factors, thus alleviating DPNP.
Effects of blue LED irradiation on the quality characteristics of Mukeunji, a long‐term fermented kimchi, during refrigerated storage
Authors: Oh Yeong Ji , Kim Selim , Lee Do Yup , Hong Jungil 
Published: January 2023

AbstractThis study investigated the effects of blue light emitting diode (BLED) irradiation on the quality characteristics of Mukeunji (long‐term fermented kimchi). Mukeunji kimchi broth (MKB) was treated with 460 nm BLED (irradiance 375 J/s∙m2) under refrigerated storage conditions. The effects of BLED irradiation on microbial growth were evaluated over the 3‐day storage period at 4°C. The quality attributes of the MKB, including color, pH, and antioxidant activity, were also assessed during the storage period. No significant difference was observed in the viable counts of lactic acid bacteria between BLED‐treated and BLED‐untreated samples. The yeast and mold counts in the MKB, however, were significantly reduced after 24 hr of BLED treatment at 4°C. The MKB pH significantly increased after 72 hr of BLED treatment at 4°C. The hunter redness (a*) was retained in BLED‐treated samples, whereas the yellowness (b*) was reduced in all samples. After BLED irradiation for 24 hr, significant changes in the 2,2′‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) radical scavenging activity of the MKB were also observed. The MJ1 and MJ2 strains isolated from MKB showed high sequence homology with Bacillus paralicheniformis and Bacillus velezensis, respectively, with 99% similarity or more in the phylogenetic tree. Chlorophyll pigment absorbs the blue light emitted by the BLED, so it can partially reduce the growth inhibitory effect of BLED irradiation on the two isolates. These findings suggest that BLED treatment can potentially modulate microbiological growth in Mukeunji during refrigerated storage without significant detriment to its characteristic properties.
Efficacy and safety of preoperative chewing gum for undergoing elective surgery: A meta‐analysis of randomised controlled trials
Authors: Chen Xinrong , Chang Chengting , Yuan Xingzhu , Yang Jie , Li Ka 
Published: January 2023

AbstractAims and ObjectivesThis meta‐analysis aimed to investigate the safety and feasibility of preoperative chewing gum in adult patients undergoing elective surgery.BackgroundPostoperative chewing gum has been shown to be safe and effective for most surgeries, while the safety and efficacy of preoperative chewing gum are still controversial.DesignA meta‐analysis of randomised controlled trials was performed.No Patient or Public ContributionThis was a meta‐analysis involving no people or animals.MethodsThe literature search was performed in 9 databases from inception to July 2022. Randomised controlled trials that compared the safety and efficacy of preoperative chewing gum and preoperative chewing no gum in adult patients undergoing elective surgery were included. The study was reported in compliance with PRISMA statement. Trial registration: PROSPERO CRD42022330223.ResultsFourteen trials involving 1433 adult patients who undergo elective surgery were pooled in this meta‐analysis. The results showed that preoperative chewing gum group resulted in no significant difference in gastric pH (p = .13) and gastric fluid volume (p = .25) compared with non‐gum‐chewing group. In comparison with the non‐gum‐chewing group, the gum‐chewing group was associated with shorter preoperative thirst score (p = .02), lower incidence of postoperative nausea (p = .0004), lower incidence of postoperative sore throat, lower incidence of postoperative hoarseness, lower postoperative pain score, shorter first postoperative anal exhaust time (p < .00001), shorter first postoperative defecation time (p < .00001) and shorter hospital days (p = .02).ConclusionsPreoperative chewing gum was associated with lower discomforts and complication rates, without increasing gastric pH and gastric fluid volume. This strategy may be an innovative, feasible and safe choice for elective surgery in adults.Relevance to Clinical PracticeThis study's results could be used as an evidence for the implementation of preoperative chewing gum in perioperative care for adult patients undergoing elective surgery.
A systematic review on price volatility in agriculture
Authors: Mustafa Zeeshan , Vitali Giuliano , Huffaker Ray , Canavari Maurizio 
Published: January 2023

AbstractThe recent extreme volatility in agriculture prices determines serious repercussions to various stakeholders and levels in the food value chain, that is, producers, intermediaries, and customers, at micro‐, meso‐ and macro‐economic governance levels, respectively. Persistent high/low degree of agriculture prices leads to unsustainable production/consumption patterns, thus representing an impediment to reaching the goal ofresponsible consumption and production(UN‐SDGs 12). The lack of comprehensive real‐time information on price volatility's internal and external factors often resulted in an inconclusive and counterintuitive outcome while performing empirical estimation. The present review was conducted using thePRISMAframework to systematically identify and analyze literature from two important databases. Papers have been grouped by volatility drivers, governance levels, theoretical approaches, and background data types. The present review is a valuable starting point for understanding the links between multi‐dimensional factors affecting the persistent price volatility and the theoretical and empirical analytics trends to provide the computational advancement needed to cope with model estimation issues. It also highlights the importance of a paradigm shift in researching agriculture price volatility to addressing food crises, considering principal objectives and perspectives such as food security, poverty alleviation, sustainability in food value chains, and resilience of food systems across the globe.
Glial cells expressing visual cycle genes are vital for photoreceptor survival in the zebrafish pineal gland
Authors: Elazary Yotam , Cheow Kathleen , Cheng Ruey‐Kuang , Ghosh Raghumoy , Shainer Inbal , Wexler Yair , Crasta Karen , Gothilf Yoav , Jesuthasan Suresh J. 
Published: January 2023

AbstractPhotoreceptors in the vertebrate eye are dependent on the retinal pigmented epithelium for a variety of functions including retinal re‐isomerization and waste disposal. The light‐sensitive pineal gland of fish, birds, and amphibians is evolutionarily related to the eye but lacks a pigmented epithelium. Thus, it is unclear how these functions are performed. Here, we ask whether a subpopulation of zebrafish pineal cells, which express glial markers and visual cycle genes, is involved in maintaining photoreceptors. Selective ablation of these cells leads to a loss of pineal photoreceptors. Moreover, these cells internalize exorhodopsin that is secreted by pineal rod‐like photoreceptors, and in turn release CD63‐positive extracellular vesicles (EVs) that are taken up by pdgfrb‐positive phagocytic cells in the forebrain meninges. These results identify a subpopulation of glial cells that is critical for pineal photoreceptor survival and indicate the existence of cells in the forebrain meninges that receive EVs released by these pineal cells and potentially function in waste disposal.
“Ye Double‐Minded”: Black Parishioners’ Attitudes toward Nonaffirming Church Climates
Authors: Young Luther 
Published: January 2023

AbstractHomophobia in the black community has been largely attributed to the Black Church's conservative views concerning sexual orientation. Prior research demonstrates that black congregations are generally nonaffirming of nonheterosexuality; however, few studies have investigated black congregants’ responses to their churches’ climates regarding sexuality. Using in‐depth interviews, this paper examines the extent to which black Christians are content with their congregations’ nonaffirming climates. Findings indicate that although some black parishioners are content with or indifferent to their churches’ climates, most respondents advocate for a change in how their congregations address sexual orientation, specifically calling for open conversations about sexuality. Furthermore, an examination of the language respondents used to evaluate congregational climates suggests discussions of sexuality in churches that humanize and encourage empathy for nonheterosexual identities may help facilitate such conversations. These results challenge assumptions of ideological homogeneity within religious organizations and could inform strategies to promote nonheterosexual inclusion in congregations.
Doxycycline serum protein binding in pigs reveals a relatively high free fraction
Authors: Portugal Felipe Ramon , Lacroix Marlène Z. , Roques Béatrice B. , Gayrard Véronique , Toutain Pierre‐Louis , Bousquet‐Mélou Alain 
Published: January 2023

AbstractDoxycycline is an antibiotic widely used in pig farming. As with all antibiotics, only the free concentrations are considered to be bacteriologically active. Historically, the free fraction (fu) in pig plasma has been estimated at 7%, which, given the effective dosage regime used in pigs, leads to free plasma concentrations of doxycycline largely lower than the minimum inhibitory concentrations of the target pathogens. This apparent inconsistency led us to reassess plasma protein binding of doxycycline in pigs. Using an equilibrium dialysis method, the extent of doxycycline binding was measured individually in 26 pigs for total doxycycline concentration ranging from 10 to 1000 μmol/L. Analysis of the data using a non‐linear mixed‐effects model demonstrated linearity of plasma protein binding with a mean fu value of 31% and a relatively low inter‐subject variability of approximately 10%. This new data showing that the free fraction is four times greater than what could have been anticipated from historical data is discussed in particular for the calculation of the PK/PD cut‐offs, which are used to establish the clinical breakpoints for antimicrobial susceptibility testing.
Prevalence of Oestrus ovis in small ruminants from the eastern Iberian Peninsula. A long‐term study
Authors: Garijo‐Toledo María Magdalena , Sansano‐Maestre José , Ahuir‐Baraja Ana Elena , Martínez‐Carrasco Carlos , de Vega Francisco Domingo Alonso , Llobat Lola , de Ybáñez‐Carnero María Rocío Ruiz 
Published: January 2023

AbstractOestrus ovis is an obligate parasite that causes myiasis in domestic ruminants, being commonly found in the Mediterranean area. From 2009 to 2019 a total of 3476 heads of culling sheep and goats from the Mediterranean coast of Spain were examined for the presence of O. ovis. The total prevalence was 56.3%, significantly higher in sheep than in goats (61.2% and 43%, respectively). Differences were found in the mean annual prevalence, with the highest value being registered in 2018 (61.7%) and the lowest in 2012 (50.3%). Autumn, for sheep, and winter, for goats, were the seasons with the highest number of infested specimens. Temperature, but not rainfall, was found to be associated with prevalence (p < 0.05). Most L1 were found in the anatomic region I (septum, meatus, and ventral conchae), while L2 and L3 were mainly located in regions II (nasopharynx, ethmoid labyrinth, and dorsal conchae), and III (sinuses). The overall intensity was 12.8 larvae per head, significantly higher in sheep (13.3) than in goats (3.5). Our results confirm the high prevalence of O. ovis in sheep and goats in this geographic area over the last decade, with the trend increasing in recent years in association with higher mean temperatures.
Strategies to build and maintain competence in pain management: Insights from a SIAARTI survey on educational needs among Italian anesthesiologists
Authors: Vittori Alessandro , Cascella Marco , Petrucci Emiliano , Cortegiani Andrea , Bignami Elena Giovanna , Innamorato Massimo Antonio , Cuomo Arturo , Torrano Vito , Petrini Flavia , Giarratano Antonino , Natoli Silvia , Marinangeli Franco 
Published: January 2023

AbstractPurposeFulfilling educational needs in pain management should be a lifelong process, even involving physicians board certified in pain medicine such as the anesthesiologists/pain therapists. The aim of the study was to investigate Italian anesthesiologists' self‐perceived competency, confidence, and interest to attend educational programs in relation to their seniority in pain management.MethodsSIAARTI members were sent an online questionnaire addressing the following items: education, skills (both soft and hard skills), technical expertise and engaged to participate between December 2020 and January 2021. Participants rated their competence based on the following range (no knowledge, knowledge, competence) while their agreement to attend educational courses was assessed using a 5‐point Likert‐type scale.ResultsLess than one in four participants declare to be dedicated to pain medicine activity with greater proportion among older (over 61 years) compared to younger ones (31–40 years). Regarding cancer and chronic noncancer pain a positive gradient of self‐perceived competence has been observed in relation to seniority. In contrast, no gradient of self‐perceived competence was reported about musculoskeletal and low back pain. Participants self‐perceived competent in both opioid use and prevention of opioid‐related adverse event while feeling less competent when managing drugs with abuse potential. The lowest competence has been observed in pediatric pain along with the lowest interest to attend educational courses. Participants were much and very much interested to education regarding cancer, noncancer, musculoskeletal, and low back pain, invasive analgesic procedures but less regarding items for which they declared less competence, such as use of pain scales, pain management in children, and use of drugs with abuse potential.ConclusionThis work provides first evidence of a summative assessment of competency and related educational needs' profile of anesthesiologists/pain therapists thus paving the way for developing a nationwide educational program to improve chronic pain care in Italy.
ABA‐triggered ROS burst in rice developing anthers is critical for tapetal programmed cell death induction and heat stress‐induced pollen abortion
Authors: Zhao Qian , Guan Xianyue , Zhou Lujian , Asad Muhammad‐Asad‐Ullah , Xu Yanqiu , Pan Gang , Cheng Fangmin 
Published: January 2023

AbstractHigh temperatures (HT) cause pollen abortion and poor floret fertility in rice, which is closely associated with excessive accumulation of reactive oxygen species (ROS) in the developing anthers. However, the relationships between accumulation of abscisic acid (ABA) and ROS, and their effects on tapetum‐specific programmed cell death (PCD) in HT‐stressed anthers are poorly characterised. Here, we determined the spatiotemporal changes in ABA and ROS levels, and their relationships with tapetal PCD under HT exposure. Mutants lacking ABA‐activated protein kinase 2 (SAPK2) functions and exogenous ABA treatments were used to explore the effects of ABA signalling on the induction of PCD and ROS accumulation during pollen development. HT‐induced pollen abortion was tightly associated with ABA accumulation and oxidative stress. The higher ABA level in HT‐stressed anthers resulted in the earlier initiation of PCD induction and subsequently abnormal tapetum degeneration by activating ROS accumulation in developing anthers. Interactions between SAPK2 and DEAD‐box ATP‐dependent RNA helicase elF4A‐1 (RH4) were required for ABA‐induced ROS generation in developing anthers. The OsSAPK2 knockout mutants showed the impaired PCD responses in the absence of HT. However, the deficiency of SAPK2 functions did not suppress the ABA‐mediated ROS generation in HT‐stressed anthers.
Structural and Functional Analysis of a Prokaryotic (6‐4) Photolyase from the Aquatic Pathogen Vibrio Cholerae†
Authors: Emmerich Hans‐Joachim , Schneider Leonie , Essen Lars‐Oliver 
Published: January 2023

AbstractPhotolyases are flavoproteins, which are able to repair UV‐induced DNA lesions in a light‐dependent manner. According to their substrate, they can be distinguished as CPD‐ and (6‐4) photolyases. While CPD‐photolyases repair the predominantly occurring cyclobutane pyrimidine dimer lesion, (6‐4) photolyases catalyze the repair of the less prominent (6‐4) photoproduct. The subgroup of prokaryotic (6‐4) photolyases/FeS‐BCP is one of the most ancient types of flavoproteins in the ubiquitously occurring photolyase & cryptochrome superfamily (PCSf). In contrast to canonical photolyases, prokaryotic (6‐4) photolyases possess a few particular characteristics, including a lumazine derivative as antenna chromophore besides the catalytically essential flavin adenine dinucleotide as well as an elongated linker region between the N‐terminal α/β‐domain and the C‐terminal all‐α‐helical domain. Furthermore, they can harbor an additional short subdomain, located at the C‐terminus, with a binding site for a [4Fe‐4S] cluster. So far, two crystal structures of prokaryotic (6‐4) photolyases have been reported. Within this study, we present the high‐resolution structure of the prokaryotic (6‐4) photolyase from Vibrio cholerae and its spectroscopic characterization in terms of in vitro photoreduction and DNA‐repair activity.
A whole‐slide imaging based workflow reduces the reading time of pathologists
Authors: Baidoshvili Alexi , Khacheishvili Mariam , van der Laak Jeroen A. W. M. , van Diest Paul J. 
Published: January 2023

AbstractEven though entirely digitized microscopic tissue sections (whole slide images, WSIs) are increasingly being used in histopathology diagnostics, little data is still available on the effect of this technique on pathologists' reading time. This study aimed to compare the time required to perform the microscopic assessment by pathologists between a conventional workflow (an optical microscope) and digitized WSIs. WSI was used in primary diagnostics at the Laboratory for Pathology Eastern Netherlands for several years (LabPON, Hengelo, The Netherlands). Cases were read either in a traditional workflow, with the pathologist recording the time required for diagnostics and reporting, or entirely digitally. Reading times were extracted from image management system log files, and the digitized workflow was fully integrated into the laboratory information system. The digital workflow saved time in the majority of case categories, with prostate biopsies saving the most (68% time gain). Taking into account case distribution, the digital workflow produced an average gain of 12.3%. Using WSI instead of conventional microscopy significantly reduces pathologists' reading times. Pathologists must work in a fully integrated environment to fully reap the benefits of a digital workflow.