bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidrug resistance plasmids underlie clonal expansions and international spread ofSalmonella entericaserotype 4,[5],12,i:- ST34 in Southeast Asia
Authors: Chung The Hao , Pham Phuong , Thanh Tuyen Ha , Vo Kim Phuong Linh , Phuong Yen Nguyen , Le Son-Nam H , Vu Thuy Duong , Chau Tran Thi Hong , Le Phuc Hoang , Ngoc Nguyen Minh , Lan Vi Lu , Mather Alison E. , Thwaites Guy E. , Thomson Nicholas R. , Baker Stephen , Pham Duy Thanh 
Published: January 2023

AbstractSalmonella entericaserotype 4,[5],12,i:- (Typhimurium monophasic variant) of sequence type (ST) 34 has emerged as the predominant pandemic genotype in recent decades. Despite increasing reports of resistance to antimicrobials in Southeast Asia,SalmonellaST34 population structure and evolution remained understudied in the region. Here we performed detailed genomic investigations on 454 ST34 genomes collected from Vietnam and diverse geographical sources to elucidate the pathogen’s epidemiology, evolution and antimicrobial resistance. We showed that ST34 has been introduced into Vietnam in at least nine occasions since 2000, forming five co-circulating major clones responsible for paediatric diarrhoea and bloodstream infection. Most expansion events were associated with acquisitions of large multidrug resistance plasmids of IncHI2 or IncA/C2. Particularly, the self-conjugative IncA/C2 pST34VN2 (co-transferringblaCTX-M-55,mcr-3.1, andqnrS1) underlies local expansion and intercontinental spread in two separate ST34 clones. At the global scale, Southeast Asia was identified as a hotspot for the emergence and dissemination of multidrug resistantSalmonellaST34, and mutation analysis suggests of selection in antimicrobial responses and key virulence factors. Our work enriches the understanding on epidemiology and evolution of this variant in Southeast Asia, and determines that multidrug resistance plasmids have driven its local and potentially global success.
Electrophysiological signatures of spelling sensitivity development from primary school age to adulthood
Authors: Larionova Ekaterina , Rebreikina Anna , Martynova Olga 
Published: January 2023

AbstractRecognizing spelling errors is important for correct writing and reading, and develops over an extended period. The neural bases of the development of orthographic sensitivity remain poorly understood. We investigated event-related potentials (ERPs) associated with spelling error recognition when performing the orthographic decision task with correctly spelled and misspelled words in children aged 8-10 years old, early adolescents aged 11-14 years old, and adults. Spelling processing in adults included an early stage associated with the initial recognition of conflict between orthography and phonology (reflected in the N400 time window) and a later stage (reflected in the P600 time window) related to re-checking the spelling. In children 8-10 years old, there were no differences in ERPs to correct and misspelled words; in addition, their behavioral scores were worse than those of early adolescents, implying that the ability to quickly recognize the correct spelling is just beginning to develop at this age. In early adolescents, spelling recognition was reflected only at the later stage, corresponding to the P600 component. At the behavioral level, they were worse than adults at recognizing misspelled words. Our data suggest that orthographic sensitivity can develop beyond 14 years.
Spatial distribution and functional relevance of FGFR1 and FGFR2 expression for glioblastoma tumor invasion
Authors: Alshahrany Nawal , Jimenez-Pascual Ana , Siebzehnrubl Florian A 
Published: January 2023

AbstractGlioblastoma is the most lethal brain cancer in adults. These incurable tumors are characterized by profound heterogeneity, therapy resistance, and diffuse infiltration. These traits have been linked to cancer stem cells, which are important for glioblastoma tumor progression and recurrence. The fibroblast growth factor receptor 1 (FGFR1) signaling pathway is a known regulator of therapy resistance and cancer stemness in glioblastoma. FGFR1 expression shows intertumoral heterogeneity and higher FGFR1 expression is associated with a significantly poorer survival in glioblastoma patients. The role of FGFR1 in tumor invasion has been studied in many cancers, but whether and how FGFR1 mediates glioblastoma invasion remains to be determined. Here, we investigated the distribution and functional relevance of FGFR1 and FGFR2 in human glioblastoma xenograft models. We found FGFR1, but not FGFR2, expressed in invasive glioblastoma cells. Loss of FGFR1, but not FGFR2, significantly reduced cell migrationin vitroand tumor invasion in human glioblastoma xenografts. Comparative analysis of RNA-sequencing data of FGFR1 and FGFR2 knockdown glioblastoma cells revealed a FGFR1-specific gene regulatory network associated with tumor invasion. Our study reveals new gene candidates linked to FGFR1-mediated glioblastoma invasion.
Non-invasive investigation of the morphology and optical properties of the upside-down jellyfishCassiopeawith optical coherence tomography
Authors: Lyndby Niclas Heidelberg , Murthy Swathi , Bessette Sandrine , Jakobsen Sofie Lindegaard , Meibom Anders , Kühl Michael 
Published: January 2023

AbstractThe jellyfishCassiopealargely cover their organic carbon demand via photosynthates produced by their microalgal endosymbionts, but how holobiont morphology and optical properties affect the light microclimate and symbiont photosynthesis inCassiopearemain unexplored. Here, we use optical coherence tomography (OCT) to study the morphology of liveCassiopeamedusae at high spatial resolution. We include detailed 3D reconstructions of external micromorphology, and show the spatial distribution of endosymbionts clustered in amoebocytes and white granules in the bell tissue. Furthermore, we use OCT data to extract inherent optical properties from light scattering white granules inCassiopeaand show that white granules enhance local light availability for symbionts in close proximity. Individual granules had a scattering coefficient of μs= 200-300 cm-1, and a scattering anisotropy factor ofg= 0.7, while large tissue regions filled with white granules had a lower μs= 40-100 cm-1, andg= 0.8-0.9. We combined OCT information with an isotopic labelling experiment to investigate the effect of enhanced light availability in whitish tissue regions. Algal symbionts located in whitish tissue exhibited significantly higher carbon fixation as compared to symbionts in anastomosing tissue (i.e., tissue without light scattering white granules). Our findings support previous suggestions that white granules inCassiopeaplay an important role in the host modulation of the light-microenvironment.
Sickle cell disease patient plasma sensitizes iPSC-derived sensory neurons from sickle cell disease patients
Authors: Allison Reilly L. , Burand Anthony , Torres Damaris Nieves , Brandow Amanda M. , Stucky Cheryl L. , Ebert Allison D. 
Published: January 2023

AbstractIndividuals living with sickle cell disease (SCD) experience severe recurrent acute and chronic pain. In order to develop novel therapies, it is necessary to better understand the neurobiological mechanisms underlying SCD pain. There are many barriers to gaining mechanistic insight into pathogenic SCD pain processes, such as differential gene expression and function of sensory neurons between humans and mice with SCD, as well as the limited availability of patient samples. These can be overcome by utilizing SCD patient-derived induced pluripotent stem cells (iPSCs) differentiated into sensory neurons (SCD iSNs). Here, we characterize the key gene expression and function of SCD iSNs to establish a model for higher-throughput investigation of intrinsic and extrinsic factors that may contribute to increased SCD patient pain. Importantly, identified roles for C-C Motif Chemokine Ligand 2 (CCL2) and endothelin 1 (ET1) in SCD pain can be recapitulated in SCD iSNs. Further, we find that plasma taken from SCD patients during acute pain increases SCD iSN calcium response to the nociceptive stimulus capsaicin compared to those treated with paired SCD patient plasma at baseline or healthy control plasma samples. Together, these data provide the framework necessary to utilize iSNs as a powerful tool to investigate the neurobiology of SCD and identify potential intrinsic mechanisms of SCD pain which may extend beyond a blood-based pathology.Key PointsSickle cell disease (SCD) stem cell derived sensory neurons (iSNs) recapitulate important SCD phenotypesin vitro.SCD patient plasma sensitizes SCD iSNs to TRPV1 stimulation by capsaicin.
Matrix vesicle isolation from a three-dimensionalin vitrobone model
Authors: Ansari Sana , van Dasler Lotte , Yuana Yuana , Castilho Miguel , Ito Keita , Hofmann Sandra 
Published: January 2023

AbstractExtracellular vesicles (EV) are nano-sized bilayer vesicles that are involved in biological functions and secreted by a wide variety of cells. Osteoblasts, the bone forming cells, can release a subset of EVs known as matrix vesicles (MtVs) which are believed to be involved in matrix mineralization and feature bone forming properties. Osteoblast-derived EVs or MtVs have been mostly isolated from conditions which are still far from nature, i.e. mesenchymal stromal cells (MSCs), or osteoblast cell lines cultured in two-dimensional (2D) tissue culture flasks. In our study, we aimed at investigating whether MtVs could also be isolated from an environment which better resembles the complexin vivosituation. This study investigated the EVs secretion during osteogenic differentiation of human bone marrow MSCs (hBMSCs) in the most advanced human three-dimensional (3D)in vitrowoven bone constructs previously developed by our group. hBMSCs were cultured in spinner flask bioreactors which induced wall shear stress on cells and directed the cells to differentiate towards osteoblasts and osteocytes. The EVs secreted into the culture medium were isolated and characterized based on their morphological, biological, and functional properties. The characteristics of a part of isolated EVs shared similarities with MtVs. These vesicles were electron-dense and electron-lucent, showed alkaline phosphatase (ALP) activity, increased the amount of released free phosphate into the culture medium, and increased the amount of deposited phosphate within the ECM. The results indicate that a complex 3D environment mimicking bone development is favorable to stimulate MtV-producing cells to produce targeted MtVsin vitro. These MtVs potentially could be used as a biological agent for bone regeneration and fracture healing through, for instance, integration with biomaterials to target bone formation locally.
Molecular evolution of toothed whale genes reveals adaptations to echolocating in different environments
Authors: Magpali L. , Ramos E. , Picorelli A. , Freitas L. , Nery M.F. 
Published: January 2023

ABSTRACTBackgroundEcholocation was a key development in toothed whale evolution, enabling their adaptation and diversification across various environments. Previous bioacoustic and morphological studies suggest that environmental pressures have influenced the evolution of echolocation in toothed whales. This hypothesis demands further investigation, especially regarding the molecular mechanisms involved in the adaptive radiation of toothed whales across multiple habitats. Here we show that the coding sequences of four hearing genes involved in echolocation (CDH23,SLC26A5,TMC1,andCLDN14) have different signatures of molecular evolution among riverine, coastal, and oceanic dolphins, suggesting that the evolutionary constraints of these habitats shaped the underlying genetic diversity of the toothed whale sonar.ResultsOur comparative analysis across 37 odontocete species revealed patterns of accelerated evolution within coastal and riverine lineages, supporting the hypothesis that shallow habitats pose specific selective pressures to sonar propagation, which are not found in the deep ocean. All toothed whales with genes evolving under positive selection are shallow coastal species, including three species that have recently diverged from freshwater lineages (Cephalorhynchus commersonii,Sotalia guianensis, andOrcaella heinsohni-CDH23), and three species that operate specialized Narrow Band High Frequency (NBHF) Sonars (Phocoena sinus-SLC26A5,Neophocaena phocaenoidesandCephalorhynchus commersonii-CDH23). For river dolphins and deep-diving toothed whales, we found signatures of positive selection and molecular convergence affecting specific sites onCDH23,TMC1,andSLC26A5. Positively selected sites (PSS) were different in number, identity, and substitution rates (dN/dS) across riverine, coastal, and oceanic toothed whales.ConclusionHere we shed light on potential molecular mechanisms underlying the diversification of toothed whale echolocation. Our results suggest that toothed whale hearing genes changed under different selective pressures in coastal, riverine, and oceanic environments.
Radial basis function neural network MPPT controller-based microgrid for hybrid stand-alone energy system used for irrigation
Authors: R. Jenitha , Rajesh K. 
Published: January 2023

Purpose
The main purpose of this controller is to carryout irrigation by the farmers with renewable energy resources.

Design/methodology/approach
The proposed design includes the Deep learning based intelligent stand-alone energy management system used for irrigation purpose. The deep algorithm applied here is Radial basis function neural network which tracks the maximum power, maintains the battery as well as load system.

Findings
The Radial Basis Function Neural Network algorithm is used for carrying out the training process. In comparison with other conventional algorithms, this algorithm outperforms by higher efficiency and lower tracking time without oscillation.

Research limitations/implications
It is little complex to implement the hardware setup of neural network in terms of training process but the work is under progress.

Practical implications
The practical hardware implementation is under progress.

Social implications
If controller are implemented in a real-time environment, definitely it helps the human-less farming and irrigation process.

Originality/value
If this system is implemented in real-time environment, every farmer gets benefitted.
An examination of the levels of work ethic in the university context across the United States of America, Korea and China
Authors: Krskova Hana , Breyer Yvonne 
Published: January 2023

PurposeThe purpose of this paper is to examine individuals' levels of work ethic amongst current and recent university attendees across three countries. This article presents the results of a survey of 537 respondents from the United States of America, Korea and China, thus extending the previous research into work ethic, often conducted from a Western perspective. The comparative study aims to enhance the understanding of cross-cultural and gender differences and similarities whilst probing for the levels of work ethic amongst the respondents.Design/methodology/approachA comparative research method was adopted because the authors' aim was to probe similarities and differences across three societies. Multiple analysis of variance (ANOVA) andt-tests were utilised to explore gender and country-related differences. Cluster analysis was applied to probe for segments highly similar to each other in the levels of work ethic of the respondents.FindingsThe results confirm the hypothesised differences between countries as well as across gender groups, with American females having the highest levels of work ethic, closely followed by Chinese males and females. Three distinct segments – low, medium and high levels of work ethic – were found in all three countries, indicating that there are individuals in each of the societies who could benefit from strategies for increasing the individuals' levels of work ethic.Originality/valueNovel gender comparisons of the three country groups revealed American females as having the highest levels of work ethic and Korean females the lowest, whilst the identification of clusters of low, medium and high levels of work ethic provides evidence of the need to increase levels of work ethic to enhance productivity, regardless of the country of origin.
The contingency effects of dependence relationship on supply chain information sharing and agility
Authors: Bai Chunguang , Govindan Kannan , Huo Baofeng 
Published: January 2023

PurposeSupply chain agility (SCA) is the primary strategy for reducing impacts and quick recovery when supply chains experience a disruption risk, such as the COVID-19 pandemic. This study will investigate how SCA can be achieved through supply chain information sharing (SCIS) under the different dependence relationships (DR) with suppliers or customers. The purpose of this paper is to investigate this issue.Design/methodology/approachBased on information process and resource dependency theories, this study constructs and empirically tests a proposed model of the relationships amongst the three dimensions of SCIS and the two areas of SCA and the contingency effects of two types of DR on those relationships. Using a dataset collected from 400 manufacturers in China, the authors tested this theoretical model using multi-group and structural path analysis.FindingsThe results of the structural path and multi-group analyses show that (1) all dimensions of SCIS are positively correlated with both areas of SCA and (2) dependence on the supplier and dependence on the customer have completely different impacts on the relationship between SCIS and SCA.Originality/valueThis study can improve the understanding of the multidimensional concepts of SCIS and SCA and relationships between them under two different DR conditions in the Chinese manufacturing setting. It contributes to IS and the SCA literature and provides theoretically driven and empirical explanations for the diverse dynamics between the dependence on the supplier and customer.
Antecedents and consequences of blockchain adoption in supply chains: a systematic literature review
Authors: Happy Anwara , Chowdhury Md Maruf Hossan , Scerri Moira , Hossain Mohammad Alamgir , Barua Zapan 
Published: January 2023

PurposeDespite the availability of several published reviews on the adoption of blockchain (BC) in supply chain (SC), at present, the literature lacks a comprehensive review incorporating the antecedents and consequences of BC adoption. Moreover, the complex adoption of BC in SC, explained with the mediating and moderating relationships, is not fully consolidated. Thus, the aim of this study was to conduct a systematic literature review (SLR) on BC technology adoption (BCTA) in SC by integrating its antecedents and consequences.Design/methodology/approachKeyword searches were performed in multiple databases resulting 382 articles for evaluation and verification. After careful screening with respect to the purpose of the study and systematic processing of the retrieved articles, a total of 211 peer-reviewed articles were included in this study for review.FindingsVarious technological, organisational, individual, social, environmental, operational and economic factors were found as the antecedents of BCTA in SC. In addition, numerous applications of BC Technology (BCT) were identified, including asset management, identity management, transaction management, data management and operations management. Finally, the consequences of BCTA were categorised as operational, risk management, economic and sustainability outcomes.Practical implicationsThis study can assist relevant decision-makers in managing the factors influencing BCTA and the potential uses of the technology to enhance SC performance.Originality/value By integrating the antecedents, applications and consequences of BCTA in SC, including the mediators and moderators, an integrated framework was developed that can potentially assist researchers to develop theoretical models. Further, the results of this SLR provide future directions for studying BCTA in supply chain management (SCM).
Culturally relevant picturebook for Middle Eastern Muslim students’ healing
Authors: Gultekin Mehmet 
Published: January 2023

PurposeIn this paper, the author adapts the concept of culturally relevant pedagogy and the pedagogy of hope for Middle Eastern Muslim students' education. This paper aims to develop a new perspective for educating Middle Eastern Muslim students by focusing on Islam by considering being Muslim as a cultural way of being and living.Design/methodology/approachPedagogy of hope (Hooks, 2003), particularly the concept of healing in education and culturally relevant pedagogy (Ladson-Billings, 1995), guided this study during the research process.FindingsThree themes of culturally relevant pedagogy through a picturebook by Mobin-Uddin (2007) entitledThe Best Eid Everwas examined to illustrate how this picturebook can be used as an example of culturally relevant pedagogy.Research limitations/implicationsThe limitation is the researcher’s interpretation as a Middle Eastern Muslim who lived in a Western country. TheBest Eid Ever(2007) can be used in the classroom for Middle Eastern Muslim students' education to discursively build a positive identity and educate students from different backgrounds. More studies may investigate other texts (e.g. novels) with Middle Eastern Muslim characters. Further research can also explore the use of this book in the classroom.Originality/valueThis study provides qualitative description of a picturebook from culturally relevant pedagogy and pedagogy of hope to guide teachers to bolster Middle Eastern Muslim students’ schooling experiences.
Analysis of procurement strategies in a two-period fresh product supply chain
Authors: Wang Guoli , Ma Chenxin 
Published: January 2023

PurposeMotivated by the wide application of procurement strategies in retailing, this paper aims to examine the effect of procurement strategies on decisions and profits and strategic inventory (SI) is considered.Design/methodology/approachThe game-theoretic models are developed under a two-period fresh product supply chain (FSC), and consist of the mode of purchasing products only in the first period without SI (Scenario S), the mode of purchasing products in every period without SI (Scenario T) and the mode of purchasing products in every period with SI (Scenario TS).FindingsConducting the calculating and comparing, some major findings can be concluded. In general, two-period purchasing strategies (Scenarios T and TS) promote a higher freshness-keeping effort than the single buying strategy (Scenario S). Regarding the pricing strategy, SI and Scenario S can both contribute to obtaining a lower wholesale price, the retailer's pricing is relatively complicated and hinges on the consumer's sensitivity to freshness-keeping effort and the holding cost. Besides, comparing the sales quantity and the profit, the authors find that Scenario TS stimulates more demands and brings more profits for the manufacturer. However, Scenario TS is not the optimal selection for the reason that SI sometimes hurts the retailer and even the whole supply chain. Whereas, when the holding cost is in a certain range, Scenario TS will lead to a win-win situation.Originality/valueThe main findings of this study can give the enterprises some advice on the procurement strategies of fresh products and the decisions of pricing and the freshness-keeping effort.