bluuebunny/crossref_metadata_2025_split huggingface dataset:
Localized activity attenuates the combined impact of a high fat meal and prolonged sitting on arterial stiffness: A randomized, controlled cross-over trial
Authors: Fryer Simon , Paterson Craig , Turner Louise , Moinuddin Arsalan , Faulkner James , Stoner Lee , Daykin Anne , Stone Keeron 
Published: February 2023

Exposure to acute prolonged sitting and consumption of a high fat (HF) meal have been shown to independently and additively impair central and peripheral cardiovascular function. This study sought to determine whether localized activity, namely leg fidgeting, offers a protective effect to these deleterious effects. Using a randomized crossover design with three trials, 18 healthy males sat uninterrupted for 180 min following the consumption of a low fat (LF, trial 1) or HF meal (trial 2). The third trial consisted of a HF meal but sitting was interrupted with 1 min of leg fidgeting (isolated bilateral plantar flexion) consisting of −250 taps per min every 5 min for the 180 min duration. Carotid-femoral pulse wave velocity (cfPWV), aortic-femoral stiffness gradient (af-SG), superficial femoral blood flow, shear-rate and PWVβ, triglyceride concentrations and lower-limb venous pooling (HHb) were assessed pre and post sitting in all trials. General linear mixed model found that following the uninterrupted HF trial, there was a significant worsening of cfPWV (mean difference (MD) = 0.57 mˑs−1; d = 1.04) and the af-SG (MD = 0.14, d = 0.50), and femoral artery blood flow (MD = 18 mlˑmin−1; d = 0.48) and shear rate (MD = 15 S1; d = 0.67) decreased. However, leg fidgeting was enough to prevent the combined deleterious effects of prolonged sitting following a HF meal. As there were no significant changes in the LF trial, the HF meal maybe the predominant driver when uninterrupted sitting is combined with a HF meal.
Computational models for prediction of protein–protein interaction in rice and Magnaporthe grisea
Authors: Karan Biswajit , Mahapatra Satyajit , Sahu Sitanshu Sekhar , Pandey Dev Mani , Chakravarty Sumit 
Published: February 2023

IntroductionPlant–microbe interactions play a vital role in the development of strategies to manage pathogen-induced destructive diseases that cause enormous crop losses every year. Rice blast is one of the severe diseases to rice Oryza sativa (O. sativa) due to Magnaporthe grisea (M. grisea) fungus. Protein–protein interaction (PPI) between rice and fungus plays a key role in causing rice blast disease.MethodsIn this paper, four genomic information-based models such as (i) the interolog, (ii) the domain, (iii) the gene ontology, and (iv) the phylogenetic-based model are developed for predicting the interaction between O. sativa and M. grisea in a whole-genome scale.Results and DiscussionA total of 59,430 interacting pairs between 1,801 rice proteins and 135 blast fungus proteins are obtained from the four models. Furthermore, a machine learning model is developed to assess the predicted interactions. Using composition-based amino acid composition (AAC) and conjoint triad (CT) features, an accuracy of 88% and 89% is achieved, respectively. When tested on the experimental dataset, the CT feature provides the highest accuracy of 95%. Furthermore, the specificity of the model is verified with other pathogen–host datasets where less accuracy is obtained, which confirmed that the model is specific to O. sativa and M. grisea. Understanding the molecular processes behind rice resistance to blast fungus begins with the identification of PPIs, and these predicted PPIs will be useful for drug design in the plant science community.
Priority planting area planning for cash crops under heavy metal pollution and climate change: A case study of Ligusticum chuanxiong Hort
Authors: He Ping , Li Yunfeng , Huo Tongtong , Meng Fanyun , Peng Cheng , Bai Ming 
Published: February 2023

IntroductionSoil pollution by heavy metals and climate change pose substantial threats to the habitat suitability of cash crops. Discussing the suitability of cash crops in this context is necessary for the conservation and management of species. We developed a comprehensive evaluation system that is universally applicable to all plants stressed by heavy metal pollution.MethodsThe MaxEnt model was used to simulate the spatial distribution ofLigusticum chuanxiongHort within the study area (Sichuan, Shaanxi, and Chongqing) based on current and future climate conditions (RCP2.6, RCP4.5, RCP6.0, and RCP8.5 scenarios). We established the current Cd pollution status in the study area using kriging interpolation and kernel density. Additionally, the three scenarios were used in prediction models to simulate future Cd pollution conditions based on current Cd pollution data. The current and future priority planting areas forL. chuanxiongwere determined by overlay analysis, and two levels of results were obtained.ResultsThe results revealed that the current first- and secondary-priority planting areas forL. chuanxiongwere 2.06 ×103km2and 1.64 ×104km2, respectively. Of these areas, the seven primary and twelve secondary counties for currentL. chuanxiongcultivation should be given higher priority; these areas include Meishan, Qionglai, Pujiang, and other regions. Furthermore, all the priority zones based on the current and future scenarios were mainly concentrated on the Chengdu Plain, southeastern Sichuan and northern Chongqing. Future planning results indicated that Renshou, Pingwu, Meishan, Qionglai, Pengshan, and other regions are very important forL. chuanxiongplanting, and a pessimistic scenario will negatively impact this potential planting. The spatial dynamics of priority areas in 2050 and 2070 clearly fluctuated under different prediction scenarios and were mainly distributed in northern Sichuan and western Chongqing.DiscussionGiven these results, taking reasonable measures to replan and manage these areas is necessary. This study provides. not only a useful reference for the protection and cultivation ofL. chuanxiong, but also a framework for analyzing other cash crops.
A psychometric evaluation of Chinese chronic hepatitis B virus infection-related stigma scale using classical test theory and item response theory
Authors: Zhong Sirui , Zhou Yuxiao , Zhumajiang Wuerken , Feng Lifen , Gu Jing , Lin Xiao , Hao Yuantao 
Published: February 2023

PurposeTo validate the hepatitis B virus infection-related stigma scale (HBVISS) using Classical Test Theory and Item Response Theory in a sample of Chinese chronic HBV carriers.MethodsFeasibility, internal consistency reliability, split-half reliability and construct validity were evaluated using a cross-sectional validation study (n = 1,058) in Classical Test Theory. Content validity was assessed by COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) criteria. The Item Response Theory (IRT) model parameters were estimated using Samejima’s graded response model, after which item response category characteristic curves were drawn. Item information, test information, and IRT-based marginal reliability were calculated. Measurement invariance was assessed using differential item functioning (DIF). SPSS and R software were used for the analysis.ResultsThe response rate reached 96.4% and the scale was completed in an average time of 5 min. Content validity of HBVISS was sufficient (+) and the quality of the evidence was high according to COSMIN criteria. Confirmatory factor analysis showed acceptable goodness-of-fit (χ2/df = 5.40, standardized root mean square residual = 0.057, root mean square error of approximation = 0.064, goodness-of-fit index = 0.902, comparative fit index = 0.925, incremental fit index = 0.926, and Tucker-Lewis index = 0.912). Cronbach’s α fell in the range of 0.79–0.89 for each dimension and 0.93 for the total scale. Split-half reliability was 0.96. IRT discrimination parameters were estimated to range between 0.959 and 2.333, and the threshold parameters were in the range-3.767 to 3.894. The average score for test information was 12.75 (information &gt;10) when the theta level reached between-4 and + 4. The IRT-based marginal reliability was 0.95 for the total scale and fell in the range of 0.83–0.91 for each dimension. No measurement invariance was detected (d-R2 &lt; 0.02).ConclusionHBVISS exhibited good feasibility, reliability, validity, and item quality, making it suitable for assessing chronic Hepatitis B virus infection-related stigma.
Psychological distress among healthcare providers in oncology during the COVID-19 pandemic in Japan: The mediating role of moral distress and resilience
Authors: Okamura Masako , Fujimori Maiko , Goto Shinichi , Ohisa Keiko , Boku Narikazu , Nakahara Rika , Uchitomi Yosuke , Suzuki Tatsuya , Matsuda Tomohiro 
Published: February 2023

ObjectiveEven though vaccines have become widespread, there is an explosion of infection due to the emergence of new mutant strains, and support for healthcare providers’ mental health is necessary. The aims of this study were to explore factors associated with the psychological distress, and to determine the degree of association between moral distress, resilience and psychological distress in order to consider intervention models for psychological distress of healthcare providers involved with cancer patients during the COVID-19 pandemic.MethodWe conducted a cross-sectional survey among healthcare providers at the National Cancer Center, Japan. Psychological distress was assessed by the Hospital Anxiety and Depression Scale. We also assessed moral distress using the Moral Distress Thermometer and resilience using the Connor-Davidson Resilience Scale 10 in April and May 2020 which was the first surge of the epidemic period.ResultsFive hundred sixty-six of 3,900 healthcare providers (14.5%) responded. Sixty-eight percent (385/566) responders were above the Hospital Anxiety and Depression Scale cutoff. Hierarchical regression analyses indicated that nurses, allied health professionals and office workers/engineers (odds ratio = 4.63; 95% confidence interval 1.90–11.29; p &lt; 0.001, odds ratio = 3.88; 95% confidence interval 1.88–8.00; p &lt; 0.001, odds ratio = 2.10; 95% confidence interval 1.06–4.18; p &lt; 0.05) as well as healthcare providers with low resilience (odds ratio = 0.88; 95% confidence interval 0.85–0.91; p &lt; 0.001) were at risk of psychological distress. Moral distress was not significantly associated with prevalence of psychological distress.ConclusionDuring the first surge of the pandemic, a high prevalence of psychological distress was demonstrated among cancer center healthcare providers. The study suggests that mental health care should be available to cancer care providers. Since the COVID-19 pandemic is still going on, in addition to the efforts by our facilities, we should consider interventions that promote resilience and a feasibility study of these interventions.
Risk perception of COVID-19 and its related factors among centralized medical isolation groups in China
Authors: Zhang Rui , Wang Chengli 
Published: February 2023

ObjectiveMedical isolation is one of the most effective measures to slow the spread of the virus when dealing with a pandemic. Millions of people in China have undergone centralized medical isolation (CMI) during the COVID-19 pandemic. This study aims to assess the centralized medical isolation group’s COVID-19 risk perception and to explore the influencing factors.MethodsA total of 400 participants (200 who had experienced CMI and 200 who had not experienced) completed a questionnaire related to COVID-19 risk perceptions. The questionnaire was designed with the Cognitive-Experiential Self-Theory (CEST) and the Common Sense Model of Risk Perception (CSM). It adopted nine questions to measure risk perception in terms of Emotional feelings, Cognitive judgment, and Mental representation of unusual severity. Descriptive statistical analysis, correlation analysis, and multiple linear regression analysis were conducted with SPSS 26.0 software.ResultsThe mean risk perception score for the CMI group was 30.75, with a standard deviation of 7.503, which was significantly higher than that in the non-centralized medical isolation (NCMI) group (risk perception score was 28.2, and the standard deviation was 7.129). The results show that risk perceptions were higher for older age, risk perceptions were higher for higher education, risk perceptions were higher for those who had received the COVID-19 vaccination, and risk perceptions were higher for those who lived in a family with children.ConclusionRisk perception is significantly higher in CMI groups than in NCMI groups. The government should draw more care to the risk perception and psychological wellbeing of the CMI group and provide extra support and assistance to the elderly and those raising younger children. In dealing with future pandemics like the COVID-19 outbreak, the government should actively guide the public to properly isolate at home and cautiously implement a CMI policy.
The influence of price value on purchase intention among patients with chronic diseases in medical e-commerce during the COVID-19 pandemic in China
Authors: Han Linlin , Han Xu 
Published: February 2023

BackgroundDuring the COVID-19 pandemic, medical e-commerce (MEC) has provided a way for patients with chronic diseases to purchase drugs online to maintain social distancing, decrease the risk of infection and community transmission, and relieve the burden on medical resources. Therefore, research which seeks to elucidate the drivers of purchase intention (PI) among patients with chronic diseases in MEC is vital. This study extended the theory of planned behavior (TPB) by integrating the price value (PV) variable into the original TPB framework and explored the effect of PV on patients' PI in MEC during the coronavirus pandemic.MethodsEmpirical data was gathered from 414 Chinese participants. Structural equation modeling was applied to explore the mechanism of chronic patients' PI in MEC. In addition, this study also estimated the moderating effect of gender, income, and region and the mediating role of attitude (ATT), subjective norm (SN), and perceived behavioral control (PBC) between PV and PI.ResultsPatients' PI in MEC is significantly affected by ATT, SN, and PBC. PV delivers significant influence on ATT, SN, PBC, and PI, with PV having the strongest effect on ATT. Gender, income, and region can significantly moderate the relationship between PV and ATT.ConclusionThese findings can contribute to design targeted interventions to increase the adoption of MEC for patients with chronic diseases, decrease infection rates, and alleviate the strain on medical resources in the COVID-19 era.
Raising effective awareness for circular economy and sustainability concepts through students' involvement in a virtual enterprise
Authors: Keramitsoglou Kiriaki , Litseselidis Theodoros , Kardimaki Anna 
Published: February 2023

IntroductionDespite increasing research on the transition of a well-established linear-oriented economic system toward the circular economy (CE) model, existing literature on the adoption and implementation of educational approaches that reinforce CE concept in secondary education seems to be limited. In light of the current challenges and the critical role of education in empowering students to explore new paths of sustainable development and grow into active citizens, conscious producers, and consumers, this study focuses on experiential learning as an effective tool for teaching CE and sustainability concepts. The literature review has revealed a research gap as regards the formulation of educational approaches to support CE concepts for secondary education students effectively, particularly in Greek vocational education. The present study describes and critically discusses how a virtual enterprise could introduce secondary-level students to the circularity and sustainability perspective, prepare them to build prosperity, and act circularly in the future.MethodsDrawing on the activities of 32 students coming from different disciplines and participants in a virtual agri-business, we recommend practical educational strategies expecting (i) to encourage teachers to adopt innovative teaching methods and share good practices of CE teaching and (ii) to urge education policymakers to integrate the CE vision into school curricula.Results and discussionFurthermore, the students' responses to a structured questionnaire before and after the programme implementation indicate that experiential learning should be supported by the teaching of theoretical aspects for a better consolidation of abstract concepts such as CE and sustainability. The implementation of a circular enterprise by a student team consists of a positive prospect for the community and economy.
Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody
Authors: Zhu Wenjun , Pickering Bradley , Smith Greg , Pinette Mathieu , Truong Thang , Babiuk Shawn , Kobasa Darwyn , Banadyga Logan , Yang Ming 
Published: February 2023

IntroductionNipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality.MethodsA competitive enzyme–linked immunosorbent assay (cELISA) using a monoclonal antibody (mAb) and recombinant NiV glycoprotein (G) was developed and laboratory evaluated using sera from experimental pigs, mini pigs and nonhuman primates. The test depends on competition between specific antibodies in positive sera and a virus–specific mAb for binding to NiV–G.ResultsBased on 1,199 negative and 71 NiV positive serum test results, the cutoff value was determined as 35% inhibition. The diagnostic sensitivity and specificity of the NiV cELISA was 98.58 and 99.92%, respectively. When testing sera from animals experimentally infected with NiV Malaysia, the cELISA detected antibodies from 14 days post–infection (dpi) and remained positive until the end of the experiment (28 dpi). Comparisons using the Kappa coefficient showed strong agreement (100%) between the cELISA and a plaque reduction neutralization test (PRNT).DiscussionBecause our cELISA is simpler, faster, and gives comparable or better results than PRNT, it would be an adequate screening test for suspect NiV and HeV cases, and it would also be useful for epidemiological surveillance of Henipavirus infections in different animal species without changing reagents.
Low-grade oncocytic tumour (LOT) of the kidney is characterised by GATA3 positivity, FOXI1 negativity and mTOR pathway mutations
Authors: Chen Tongbing , Peng Yan , Lei Ting , Wu Chao , Wang Hui , Shi Yongqiang 
Published: February 2023

Aims: We present a 5-case series of low-grade oncocytic tumour of the kidney to further discuss their clinicopathological characteristics.Methods and results: Five patients were included in this study. There were three females and two males aged 45–66 years, with a median age of 65 years. Four tumours were located in the right kidney, and one was located in the left kidney. Most of the tumour sections were yellow-brown in colour. Tumour sizes ranged from 2.5 to 4.5 cm, with a median size of 3 cm. Microscopically, the tumours were well-circumscribed but lacked a fibrous capsule; the tumours consisted of monomorphous oncocytic cells arranged mainly in solid and nested architectural patterns. The tumour cells had uniformly round to oval nuclei and often had perinuclear halos but lacked significant irregularities. Immunohistochemically, the tumour cells showed a diffuse and strong positivity for CK7 and were negative for CD117. The tumour cells were also positive for GATA3, E-cadherin, Pax-8, Succinate dehydrogenase B (SDHB) and Fumarate hydratase (FH), and negative for vimentin, Carbonic anhydrase 9 (CA9), CD10, P504s, CK20, TFE3, TFEB, HMB45, ALK and Forkhead box protein I1 (FOXI1). Next-generation sequencing identified genetic variations in these tumours, including MTOR gene mutations (4/5) and PIK3CA gene mutation (1/5). All patients were alive without disease progression at a median follow-up of 32 months (range 10–57 months).Conclusion: LOT is an emerging renal entity of indolent behaviour that has morphologic overlap with some renal tumours with eosinophilic cytoplasm, primarily with oncocytoma and eosinophilic variant of chromophobe renal cell carcinoma. Familiarity with the distinctive morphological features, immunophenotype and molecular genetics of LOT helps avoid misdiagnosis.
The Curious Case of Fritillaria sonnikovae (Liliaceae) in South Siberia: New Insights into Its Origin and Phylogeny
Authors: Protopopova Marina , Sandanov Denis , Pavlichenko Vasiliy , Selyutina Inessa , Stepanov Nikolay 
Published: February 2023

Fritillaria Tourn. ex L. is a genus of Liliaceae including a little more than 150 species occurring in the temperate Holarctic. Fritillaria sonnikovae Shaulo & Erst is the most recently described Siberian species in the genus. In the affinity section of the F. sonnikovae diagnosis, only F. dagana Turcz. and F. roylei Hook. are mentioned. Our study is an original attempt to shed light on the F. sonnikovae origin and its evolutionary relationships with other Fritillaria using nuclear (ITS) and plastid (matK + rps16 + trnH-psbA) DNA markers. Our results showed that F. sonnikovae together with F. dagana and F. maximowiczii Freyn belongs to the North Asian lineage of the Liliorhiza subgenus and produced no evidence supporting relationship between F. sonnikovae and F. roylei. Monophyly of Fritillaria sonnikovae was not reliably confirmed in our study since its close affinity with F. maximowiczii was demonstrated by phylogenetic analysis and morphology. Fritillaria dagana was shown to be a sister to the F. maximowiczii + F. sonnikovae group. Most authors of the present study suggest considering F. sonnikovae as a synonym for F. maximowiczii. In this view, F. sonnikovae may be considered a narrow endemic and one of the light-perianth morphs of F. maximowiczii, which has emerged in the Western Sayan and remained there as a tertiary relict.