bluuebunny/crossref_metadata_2025_split huggingface dataset:
Post-acute phase Rac1/Pak1/p38/β-catenin signalling activation promotes ischemic recovery and facilitates migration of neural stem cells
Authors: Wu Fan , Xu Zihan , Zhang Luyuan , Li Ganglei , Lin Hongwei , Shen Jie , Zhou Lihui , Liu Zongchi , Shen Jian , Zhan Renya , Gong Jiangbiao , Zhu Yu 
Published: January 2023

Abstract
Ischemic stroke is one of the world's leading causes of death and disability. During ischemic stroke, neuronal death is caused by inflammation, oxidative stress, apoptosis, and excitatory toxicity. For a long time, there has been a lot of interest in various studies to promote the repair of nerve damage after stroke. However, the mechanisms underlying the complex pathophysiological processes remain to be fully elucidated. Cerebral Ras-related C3 botulinum toxin substrate (Rac) 1 was initially associated with poor prognosis after stroke. However, some recent studies suggest that Rac1 may contribute to the repair process of nerve injury after stroke and promote the recovery of nerve function. However, the current conflicting findings may be related to the timing of Rac1 interventions. Our study found that post-acute Rac1 activation contributes to ischemic recovery. Here, we first investigated the specific role of neuron Rac1 in neuroprotection and recovery of neurological function in the post-acute phase of stroke. We found that intraventricular injection of Rac1 activator beginning one week after MACO significantly improved brain atrophy and neurological function after MACO. Considering the important role of Rac1 in cell migration. In vitro experiments, we found that Rac1 induced NSCs migration after exogenous and endogenous activator stimulation. In vivo, the addition of Rac1 activator in the post-acute phase helped promote the migration of NSCs in SVC to the lesion area and differentiation into mature nerve cells. It is worth noting that Pak1 is an essential downstream protein of Rac1. Our results suggest that while Rac1 activates Pak1, Pak1 can promote the migration and differentiation of neural stem cells through the downstream p38/β-catenin signalling pathway. While promoting neurogenesis, we found that Rac1 promoted angiogenesis and axon remodelling, and enhanced the synaptic plasticity to support the process of nerve repair during the post-acute phase via downstream Pak1/p38/β-catenin signalling. It suggests that Rac1/Pak1/p38/β-catenin signalling may be a potential therapeutic target in the recovery stage of ischemic stroke.
Unusual magnetotransport and surface Dirac-cone state in single-crystalline Pt 3 Te 4 : a new candidate of Dirac semimetal
Authors: Hsu Kuan , Chen I Nan , Kuo Chia Nung , Lei Zhujialei , Lue Chin Shan , Wang Li-Min 
Published: January 2023

Abstract

Exotic magnetotransport and magnetization properties of Pt3Te4 single crystals were investigated to probe the topological properties of the Pt3Te4 semimetal. Pt3Te4 reveals a strongly correlated electronic feature as well as a non-trivial magnetoresistance (MR) characteristic. The signature of helical spin texture from the topological surface state and the chiral anomaly associated with a linear-like energy dispersion of electronic states were detected. At low temperatures, the negative longitudinal magnetoresistance in the low-field region could be explained with the transport formula containing the chiral-anomaly effect as well as the weak antilocalization transport. Moreover, the high-field transverse magnetoresistance at temperatures below 60 K showed a non-saturating linear-like behavior, which was examined with the theory of Abrikosov’s quantum MR, indicating a Dirac-cone-like dispersion in Pt3Te4 at low temperatures. This work reveals the drastic impact of the concept that the magnetotransport in Pt3Te4 can be dominated by the surface electrons in a Dirac fermion state, thus inferring that Pt3Te4 is a new candidate of Dirac semimetal.
Comparison of two high dose rate Intracavitary brachytherapy regimens in treatment of cervical cancer
Authors: Krishna Abhishek , AG Hasib , Fernandes Donald , MS Athiyamaan , Rao Sandesh , Shankar Sharaschandra , Ali Mohsina , Priyadarshini Hepsiba , Sophia Maria , CH Shridhar , George Seby , Babu Amrutha , Banerjee Sourjya , Sunny Johan , Srini Challapalli , Lobo Dilson 
Published: January 2023

Abstract
Background: To assess and compare the local control and toxicities between HDR Intracavitary Brachytherapy with 7.5 Gy and 9 Gy per fraction after EBRT in treatment of carcinoma cervix.
Methodology: A total of 180 patients were randomly assigned to 2 arms. Arm A received HDR intracavitary brachytherapy with a dose of 7.5 Gy per fraction, 1 fraction per week for 3 fractions and Arm B received 9 Gy per fraction, 1 fraction per week for 2 fractions. Patients were evaluated on follow up for assessment of local control and toxicities.
Results: The median follow up was 12 months (6-18 months). In arm A 89% of the patient had complete response and 11 % had recurrence or metastasis. In arm B 93% of the patient had complete response and 7 % had recurrence or metastasis. Grade 2/3 diarrhoea was seen in 4.4% of patients in Arm A and in 7.7% in Arm B. Grade 2/3 proctitis was seen in 3.3 % of patients in 7.5 Gy arm and in 6.6 % in 9 Gy arm. One patient in each arm had grade 1 haematuria. The overall duration of treatment was significant lower in Arm B compared to Arm A (59 days vs 68 days, p=0.01)
Conclusion: The result of this clinical study shows that Intracavitary brachytherapy with a dose of 9 Gy per fraction is non inferior to other schedules in term of local control and does not result in increased toxicity
Diaries as technologies for sense-making and self-transformation in times of vulnerability.
Authors: Marcos Marcos José Bernal , Zittoun Tania , Gillespie Alex 
Published: January 2023

Abstract
Diaries have been generally understood as “windows” on sense-making processes when studying life ruptures. In this article, we draw on Michel Foucault’s conceptualization of self-writing as a “technology of the self” and on sociocultural psychology to propose that diaries are not “windows,” but technologies that aid in the sense-making. Concretely, we analyzed three non-exhaustive and non-exclusive uses of diary writing in times of vulnerability: 1) imagination of the future and preparation to encounter difficulties; 2) distancing from one's own experience; and 3) creating personal commitments. Our longitudinal data comprised three public online diaries written over more than twenty years, belonging to three anonymous individuals selected from a database of more than 400 diaries. We analyzed these three diaries by iterating between qualitative and quantitative analysis. We conclude that: 1) beyond their expressive dimension, diaries are technologies that support the sense-making process, but not without difficulties; 2) diaries form a self-generated space for dialogue with oneself in which the diarist also becomes aware of the social nature of her life story; 3) diaries are not only technologies for the Socratic “know thyself” but also technologies to work on oneself, especially in terms of the personal perspective on the past or the future; and 4) the practice of diary writing goes beyond sense-making towards personal development and the desire to transform one's life trajectory.
VE-822 upregulates the deubiquitinase OTUD1 to stabilize FHL1 to inhibit the progression of lung adenocarcinoma
Authors: Zhang Qi , Li Jinglei , Chen Zihan , Jiang Ke , Yang Kunyu , Huang Fang , Huang Ai , Zhang Xiaodong , Zhang Jinxiang , Wang Hui 
Published: January 2023

Abstract
Background
The deubiquitinase ovarian tumor domain-containing 1 (OTUD1) has been considered as a tumor suppressor in many tumors, but there is minimal research on the role of OTUD1 in lung adenocarcinoma (LUAD) pathogenesis.
Methods
Bioinformatics analyses and western blot were applied for investigating OTUD1 expression in lung cancer and the drug that upregulated OTUD1. Kaplan–Meier analysis with log-rank test was used for survival analyses. IP-MS and co-IP were performed for identifying potential protein interactions with OTUD1. In vitro and in vivo assays were used for exploring the function of OTUD1 during the progression of LUAD.
Results
OTUD1 was dramatically downregulated in tumors and cell lines of human lung cancer. OTUD1 inhibited proliferation and migration of lung cancer cells in vitro. Moreover, OTUD1 inhibited growth of xenografts in nude mice and formation of primary lung tumors in urethane-induced lung cancer model. Mechanistically, we showed that OTUD1 deubiquitinated and stabilized FHL1. Furthermore, we listed and identified VE-822 as a candidate agonist for OTUD1. VE-822 inhibited proliferation of lung adenocarcinoma both in vitro and in vivo.
Conclusions
These results indicated that the deubiquitinase OTUD1, which was upregulated by VE-822, inhibited the progression of LUAD in vitro and in vivo by deubiquitinating and stabilizing FHL1.
Research of histological characteristics and single nucleotide polymorphisms in hereditary genes among Pakistani breast cancer patients
Authors: Nawaz Yasir , Khan Ali Zaib , Tanvir Fouzia , Ambreen Sadaf , Zafar Javaria , Umar Asma , Majeeed Imran , Iqbal Alia , Ayoub Ambar 
Published: January 2023

Abstract
Cancer is a type which involves irregular progression of cells with the capacity to enter and move to other portions of body. Breast cancer starts from breast tissues, mostly from inner coating of milk ducts. It is categorized into various methods by, grade, stage and receptors status. It is very common in females worldwide. Whole-exome sequencing (WES) was done in DNA removed from tumors of six breast cancer patients from Jinnah hospital Lahore, Pakistan. There were 19 patients from age 27 to 73 from which tissue sample were collected from 6 patients. The age distribution shows non-significant differences. The ER/PR status shows non-significant differences and odds ratio equals to 1. Somatic mutations were detected in three targeted genes ATM, TP53 and CDH (CDH5, CDH8, CDH10, CDH12, CDH16, CDH20, CDH23 and CDH24) in sample 1. Two genes with exonic variants were found in sample 2 containing TP53 and CDH (CDH5, CDH16, CDH19, and CDH23). Amino acid change and deletions were observed in different exonic sites of these genes. To conclude, more number of patients was observed having invasive ductal breast carcinoma. A number of novel somatic mutations for breast cancer were recognized. More studies are needed to define the functions of these mutated genes in breast cancer. Whole exome sequencing shows different type of mutations in different exonic regions of genes including TP53, ATM and CDH.
Self-pacing and multiple-attempt assessment to address student anxiety in pharmacy calculations
Authors: Bell Edward C. 
Published: January 2023

Abstract
Objective.

Pharmacy calculations is a course that can be challenging and is often associated with student anxiety about assessments and grades. This study was conducted to determine if student anxiety would be reduced in pharmacy calculations using self-paced, multiple-attempt assessments.

Materials and Methods.

Self-paced, multiple-attempt assessments were presented to students as graded practice modules and as examinations. Pre-post surveys were used to measure student anxiety in pharmacy calculations. Module performance indices and exam performance indices were correlated with course grade outcomes.

Results.

Fifty-four students participated in pre-surveys and forty-eight students participated in post-surveys. Westside Test Anxiety Scale survey results indicated use of self-paced, multiple-attempt assessments reduced students’ perceived anxiety about pharmacy calculations. Student comments about assessment methods were predominately positive. Course grades strongly correlated with module mean scores and high scores, and strongly correlated with mean exam scores. A negative correlation between course grades and belated module attempts alluded to harmful association between student procrastination and course performance in weaker students.

Conclusions.

Self-paced, multiple-attempt assessment was associated with improved perception of student test anxiety about pharmacy calculations. With care taken to limit student procrastination, use of these types of assessment could be an effective means of improving student comfort while promoting mastery of the subject.
Mechanistic insights into the anti-depressant effect of curcumin based on network pharmacology and experimental validation
Authors: Guo Jing , Fang Meng , Xiong Zhe , Zhou Ke , Zeng Peng 
Published: January 2023

Abstract

Growing evidence supports the involvement of neuroinflammation in the pathophysiology of depression. Administrating curcumin could revert the depressive-like symptoms and weakened microglial activation and increased the level of pro-inflammatory cytokine. This study aimed to identify potential anti-depression targets and mechanisms of curcumin (CUR) by an approach of network pharmacology. GSEA and KEGG pathways showed the most significantly enriched pathway of CUR against depression was the PI3K-Akt pathway. Moreover, 52 targets were significantly correlated with PI3K-Akt signaling pathway and CUR-related targets. In addition, among these top 50 targets which were ranked by degree in the PPI network, there were 23 targets involved in the 52 intersection targets. Thus, our findings suggest that CUR exerts its anti-depression effects through PI3K-Akt signaling pathway. Furthermore, we investigated the anti-depression effects of CUR using a mouse model of depression induced by lipopolysaccharide (LPS). Administration of LPS alone (2 mg/kg/day, i.p.) extended the immobility time in the open filed test (OFT) and tail suspension test (TST), decreased sucrose consumption in the sucrose preference test (SPT). Pretreatment with CUR (50 mg/kg/day, i.p.) for 7 consecutive days relieved LPS-induced changes in the behavior tests, the activity of PI3K-Akt signaling pathway, neuronal damage in the PFC and inflammatory response. Moreover, inhibition of the PI3K-Akt signaling pathway by LY294002 (7.5 mg/kg/day, i.p.) blocks the therapeutic effects of CUR. In conclusion, our study indicate that CUR may be an effective antidepressant agent for LPS-induced mouse model, in part because of its anti-inflammatory actin through PI3K-Akt signaling pathway.
Assessing and comparing crop evapotranspiration in different climatic regions of China using reanalysis products
Authors: Yu Xingjiao , Qian Long , Wang Wen’e , Hu Xiaotao , Huo Xuefei , Wang Yafei 
Published: January 2023

Abstract
This study aims to assess the accuracy of the crop reference evapotranspiration (ET0 CLDAS, ET0 ERA5) estimated by CLDAS, ERA5 reanalysis products, as well as the quality of reanalysis weather variables required to calculate PM-ET0, and to achieve the application of these reanalysis products to locations where weather data quality are low or (and) weather variables are missing. For this purpose, the applicability of surface meteorological elements such as daily maximum and minimum air temperatures, relative air humidity, 2m wind speed, and shortwave radiation from the ERA5 reanalysis datasets provided by the European Centre for Medium-Range Weather Forecasts (ECMWF), and the second-generation China Meteorological Administration Land Data Assimilation System (CLDASV2.0) datasets are evaluated in China by comparison with local observations from 689 stations reported by the Chinese Meteorological Administration (CMA). Statistical statistics including percent bias (PBias), coefficient of determination (R2), root mean square error (RMSE) and mean absolute error (MAE) are used to check the accuracy. The results show the highest correlation between reanalysis temperature and station observations, with a mean R2 of 0.96,0.90 for CLDAS reanalysis maximum and minimum air temperatures and 0.87,0.84 for ERA5. For the reanalysis of estimated solar radiation and relative humidity, an overestimation trend is shown for Rs, but to a lesser degree, an underestimation trend is shown for RH. Unlike the previous reanalysis variables, the reanalysis wind speed shows a lower accuracy, and average R2 = 0.25 (R2 = 0.18) for CLDAS reanalysis (ERA5 reanalysis) and site observations. In addition, the accuracy of ET0 estimated by the two reanalysis products is acceptable in China, but the spatial and temporal consistency between CLDAS estimates and site observations is higher, with mean RMSE, R2 of 0.91,0.82 for ET0 CLDAS and 1.42, 0.70 for ET0 ERA5, respectively, and the performance of describing the boundary details of the study area is better since CLDAS reanalysis products integrate terrain adjustment, the elevation of target location, wind speed, and other factors are taken into account.
Increased serum soluble interleukin-2 receptor levels in dermatomyositis are associated with Th17/Treg immune imbalance
Authors: Xie Yuhuan , Zhang Tingting , Su Rui , Liu Lu , Jiang Lei , Xue Hongwei , Gao Chong , Li Xiaofeng , Wang Caihong 
Published: January 2023

Abstract
Objective: In patients with dermatomyositis/polymyositis (DM/PM), especially DM patients with positive antibodies against melanoma differentiation-associated protein 5 (MDA5), the prognosis is very poor, acute progressive pulmonary interstitial disease is likely to occur, and the mortality rate is very high, while serum soluble interleukin-2 receptor (sIL-2R) is often used as a marker to assess T cell activation, it is still poorly understood. The aim of this study was to investigate the relationship between sIL-2R levels and disease activity, absolute number of peripheral blood lymphocyte subsets and related cytokines in DM patients.
Method: Sixty patients with DM (32 patients with inactive DM and 28 patients with active DM) were enrolled in this study and divided into inactive and active groups according to the Myositis Disease Activity Visual Analogue Scale (MYOACT), and the absolute numbers of peripheral lymphocyte subsets and CD4 + T cell subsets were measured by flow cytometry in each group, and serum cytokine levels were measured by flow cytometry bead array.
Results: Serum sIL-2R levels were positively correlated with independent visual analogue scale (VAS) in DM patients (p < 0.001), and the ratio of Th17/Treg cells was significantly higher in DM patients compared with the healthy group (P < 0.01), and there was a correlation between serum sIL-2 levels and Th17/Treg ratio. Multivariate logistic regression revealed that serum sIL-2R levels were an independent factor affecting disease activity. Serum IL-6 and IFN-γ levels were also increased in the active group compared with the inactive group (p = 0.011 and p = 0.034, respectively). In addition, receiver operating characteristic (ROC) curves showed that serum sIL-2R levels contributed to the discrimination of disease activity in DM patients, with an area under the ROC curve (AUC) of 0.757 (95% CI 0.630 – 0.884, P = 0.001).
Conclusion：In DM patients, serum sIL-2R levels are not only closely related to disease activity, but also involved in their Th17/Treg immune imbalance, which is an effective indicator for evaluating DM disease activity.
Neurobehavioral deficits, histoarchitectural alterations, parvalbumin neuronal damage and glial activation in the brain of male Wistar rat exposed Landfill leachate
Authors: Levi Usende Ifukibot , Bintu Mofio M. , Daniella Osinachi Chinonyerem , Oyenike Oyelowo-Abdulraheem Fatima , Agbonu Adikpe Oluwa , Adedamola Azeez Mariam , Ndidi Enefe , Mbagwu Smart , Edem Edem Ekpenyong , Olukayode Olopade James , James Connor , Sanni Fatimah Saka 
Published: January 2023

Abstract

Concerns about inappropriate disposal of waste into unsanitary municipal solid waste landfills around the world has been on the increase; and this pose a public health challenge due to leachate production. The neurotoxic effect of Gwagwalada landfill leachate (GLL) was investigated in male adult Wistar rats. Rats were exposed to 10% concentration of GLL for 21days. Control group received tap water for same period of experiment. Our results showed that neurobehavior, absolute body and brain weights and brain histomorphology as well as parvalbumin interneurons were severely altered, with consequent astrogliosis and microgliosis after 21 days of administrating GLL. Specifically, there was severe loss and shrinkage of purkinje cells, with their nucleus, and severe diffused vacuolations of the white matter tract of GLL exposed rat brains. There was severe cell loss in the granular layer of the cerebellum resulting to reduced thickness of the layer. Also. there was severe loss of dendritic arborization of the purkinje cells in GLL exposed rat brains, and damage as well of reduced populations of parvalbumin-containing fast-spiking GABAminergic interneurons in various regions of the brain. In conclusion, data from the present study demonstrated the detrimental effects of Gwagwalada landfill leachate on the brain which may be implicated in neuropsychological conditions.
Identifying the diagnostic value of inflammatory genes in intracranial aneurysms based on machine learning
Authors: Zhou Min , Fu Ya , Liang RiChu , Duan YongHong , Liu XiaoFei , Luo Jie , Jiang YuanDing , Liao Quan 
Published: January 2023

AbstractBackground Intracranial aneurysm (IA) is a cerebrovascular disease that can be caused by a variety of factors. Clinical trials have indicated that inflammation and inflammatory cells play critical roles in the pathophysiology of IA. Nonetheless, the roles of inflammation-related genes (IRGs) in IA remain unclear. Methods The GSE75436 and GSE54083 datasets were acquired from the Gene Expression Omnibus (GEO) database, and the IRGs were extracted from the MSigDB database. First, the two GEO datasets were combined, and the batch effects were removed. The differentially expressed inflammation-related genes (DEIRGs) were identified by overlapping the IRGs with the set of differentially expressed genes (DEGs) between IA and control samples. The functions of the DEIRGs were evaluated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Then, ROC curve analysis was used to verify the diagnostic ability of the DEIRGs, and diagnostic models were constructed with 7 machine learning methods. Furthermore, gene set enrichment analysis (GSEA) was performed to explore the potential biological functions of the biomarkers. Immune cell relevance was assessed by single-sample gene set enrichment analysis (ssGSEA). In addition, a TF–mRNA‒miRNA network was established, and potential biomarkers were predicted. Ultimately, the mRNA expression levels of the biomarkers were validated by quantitative real-time PCR (qRT‒PCR). Results In total, 35 DEIRGs were retrieved by overlapping the 964 DEGs and 200 IRGs. Functional enrichment analysis revealed that the DEIRGs were significantly enriched in the regulation of the inflammatory response, immune receptor activity, and lipid and atherosclerosis pathways. Moreover, 13 genes with an AUC greater than 0.85 were selected for diagnostic model construction by the RF algorithm, and 7 biomarkers were obtained in the final model. GSEA indicated that these 7 biomarkers were mainly associated with inflammation. The significantly differentially abundant immune cells exhibited positive correlations with the biomarkers. Subsequently, we proposed that SERPINE might be modulated by TBX3, MLX, and NR1I3 and that SLC11A2 might be modulated by hsa-miR-6838-5p, hsa-miR-4784, and hsa-miR-3663-5p. In addition, 22 drugs were predicted to interact with the biomarkers, including fluoxetine, aleplasinin, and orlistat. Finally, qRT‒PCR results showed that the expression levels of the 7 biomarkers were significantly higher in IA tissue than in superficial temporal artery tissue. Conclusion This research provides a new perspective for understanding the molecular mechanism of IA pathogenesis and valuable evidence for the pathological diagnosis of IRGs.