bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mechanics theory for the exploration of a high-throughput, sterile 3D in vitro traumatic brain injury model
Authors: Wan Yang , González-Cruz Rafael D. , Hoffman-Kim Diane , Kesari Haneesh 
Published: September 2023

Abstract
Brain injuries resulting from mechanical trauma represent an ongoing global public health issue. Several in vitro and in vivo models for traumatic brain injury (TBI) continue to be developed for delineating the various complex pathophysiological processes involved in its onset and progression. Developing an in vitro TBI model that is based on cortical spheroids is especially of great interest currently because they can replicate key aspects of in vivo brain tissue, including its electrophysiology, physicochemical microenvironment, and extracellular matrix composition. Being able to mechanically deform the spheroids is a key requirement in any effective in vitro TBI model. The spheroids’ shape and size, however, make mechanically loading them, especially in a high-throughput, sterile, and reproducible manner, quite challenging. To address this challenge, we present an idea for a spheroid-based, in vitro TBI model in which the spheroids are mechanically loaded by being spun by a centrifuge. (An experimental demonstration of this new idea will be published shortly elsewhere.) An issue that can limit its utility and scope is that imaging techniques used in 2D and 3D in vitro TBI models cannot be readily applied in it to determine spheroid strains. In order to address this issue, we developed a continuum mechanics-based theory to estimate the spheroids’ strains when they are being spun at a constant angular velocity. The mechanics theory, while applicable here to a special case of the centrifuge-based TBI model, is also of general value since it can help with the further exploration and development of TBI models.
Cytotoxicity effect, network pharmacology, molecular docking, and molecular dynamics simulation of new mono-chalcone compounds for breast cancer
Authors: Ismail Noor Zafirah , Khairuddean Melati , Alidmat Mohammad Murwih , Abubakar Sadiq , Arsad Hasni 
Published: September 2023

Abstract
Chalcones are flavonoids' derivatives and have a wide range of biological functions. Chalcones' anticancer efficacy arises from their capacity to operate on several targets. Hence, the purpose of this study was to examine cytotoxicity, network pharmacology, molecular docking, and molecular dynamics (MD) simulations of newly synthesized mono-chalcone compounds related to breast cancer. To achieve this objective, the cytotoxicity of mono-chalcone compounds in relation to breast cancer cells, specifically MCF-7 and MDA-MB-231, was investigated. The results demonstrated that the compounds were significantly suppressed in both breast cancer cells. Subsequently, the compounds were subjected to a network pharmacology analysis. The findings showed that both the compounds and the breast cancer target network shared 160 protein targets. In addition, it was discovered that most of the targeted proteins are involved in cancer pathways. The apoptosis proteins known as BCL2, MDM2, and CASP3 were the enriched genes identified in this analysis. Molecular docking analysis showed that the compounds had high binding affinities for their respective protein receptors. Therefore, the protein-ligand conformations were subjected to a 100 ns MD simulation at 310 K. Comparatively to the reference proteins, the protein-ligand conformation complexes exhibited greater stability, compactness, and negligible structural changes. The results indicate that both mono-chalcone compounds are of great significance and shed light on the molecular interactions between these compounds and proteins involved in the apoptosis breast cancer pathway.
Caracterização química, biológica e nutricional de plantas alimentícias não convencionais do gênero Solanum L. (Solanaceae)
Authors: Moreira de Sousa Welinton Gustavo , de Abreu Maria Carolina 
Published: September 2023

As plantas alimentícias não convencionais (PANCs) são espécies não habitualmente utilizadas na alimentação humana, consideradas inços e matos, mas que são fonte de nutrientes e metabólitos secundários. Contudo, tais plantas não são exploradas na alimentação, por causa do desconhecimento dos métodos de cultivo e preparo, do perfil nutricional e da segurança de consumo. À vista disso, o presente estudo realizou um levantamento bibliográfico acerca de seis espécies do gênero Solanum (Solanaceae), classificadas como PANC, objetivando, com isso, destacar os seus potenciais nutracêuticos, fitoquímicos, toxicológicos e biológicos. A pesquisa bibliográfica deu-se a partir do levantamento de livros e artigos científicos nos bancos de dados PubMed, Science Direct, EMBASE e Biblioteca Virtual de Saúde (BVS), obedecendo ao recorte temporal de 2000 a 2020. Dentre 1.447 periódicos levantados, foram selecionados 68, visto que esses, na análise empreendida, contemplavam os critérios metodológicos e, dessa forma, foram utilizados na caracterização das espécies. A literatura aponta que as espécies são ricas em nutrientes e metabólitos secundários, já que possuem potencialidades no combate a diversas enfermidades. Ademais, a literatura chama a atenção para grupos químicos e elementos tóxicos presentes em alguns representantes do gênero Solanum. Os resultados denotam, em suma, o potencial das espécies para o desenvolvimento de ingrediente farmacêutico e de alimentos funcionais com impacto benéfico à saúde humana.
Características físico-químicas de frutos de Myrcianthes pungens (O.Berg) D. Legrand
Authors: Veit Pedro Augusto , Schwarz Sergio Francisco , Guerra Divanilde , Simioni Carine , Pezzi Ernani 
Published: September 2023

O guabijuzeiro produz frutos com potencial para exploração comercial, porém poucos estudos foram conduzidos. O objetivo deste trabalho foi caracterizar frutos de guabijuzeiros coletados em diferentes locais do Rio Grande do Sul. Os frutos de 13 acessos foram avaliados, em duas safras, quanto à massa fresca (MF), diâmetro longitudinal (DL) e equatorial (DE), cor, rendimento de polpa (RP), sólidos solúveis totais (SS), acidez total (AT) e Vitamina C. Na primeira safra o acesso G1 destacou-se em MF (6,89 g), DL (19,4 mm) e DE (22,3 mm); já na segunda safra os melhores resultados para MF foram obtidos com os acessos P1 (4,93 g) e BG2 (4,80 g), para DL com os acessos P1 (17,86 mm) e BG2 (17,40 mm) e DE com os acessos BG2 (20,25 mm) e P1 (20,17 mm), enquanto para o RP, as médias foram de 53,18 e 53,0%, na primeira e segunda safras, respectivamente. A cor predominante da casca foi a cinza. O teor de SS apresentou média de 15,8 e 15,1 °Brix na primeira e segunda safras, respectivamente. Para AT, as médias foram de 0,15% e 0,18%, na primeira e segunda safras, respectivamente. Para as relações SS/AT as médias foram de 108 e 91, na primeira e segunda safras, respectivamente. Para os teores de Vitamina C o acesso G1 se destacou na primeira (42,3 mg/100 g de polpa) e segunda safras (41,9 mg/100 g de polpa). Portanto, alguns acessos apresentam potencial para serem propagados visando à utilização em coleções e programas de melhoramento genético.
Effect of Statins on Superoxide Dismutase Level: A Systematic Review
Authors: Daliri Mahla , Simental-Mendia Luis E. , Jamialahmadi Tannaz , Kesharwani Prashant , Reiner Zeljko , Sahebkar Amirhossein 
Published: September 2023

Background and Objective:
The literature suggests that statins may increase
superoxide dismutase (SOD) levels by different mechanisms. These effects may contribute
to the antioxidant and anti-inflammatory effects of statins, which are thought to be
beneficial in preventing cardiovascular events. However, there are also conflicting results
concerning the effect of statins on SOD levels. The goal of this systematic review was to
evaluate the effect of statin therapy on SOD activity.

Methods:
This systematic review was performed based on the PRISMA statement. The terms ("statin" or "HMG-CoA reductase inhibitor" OR "lipid-lowering agents" OR "Atorvastatin" OR "Simvastatin" OR "Pravastatin" OR "Fluvastatin" OR "Lovastatin") AND ("superoxide dismutase" OR "SOD" OR "anti-oxidative" OR "oxidative stress") were searched in database systems Google Scholar, PubMed/MEDLINE, and Scopus from inception to April 2022.

Results:
This systematic review was performed based on the PRISMA statement. The
terms (“statin” or “HMG-CoA reductase inhibitor” OR “lipid-lowering agents” OR “Atorvastatin”
OR “Simvastatin” OR “Pravastatin” OR “Fluvastatin” OR “Lovastatin”) AND
(“superoxide dismutase” OR “SOD” OR “anti-oxidative” OR “oxidative stress”) were
searched in database systems Google Scholar, PubMed/MEDLINE, and Scopus from inception
to April 2022.

Conclusion:
Our systematic review suggests that treatment with statins has a positive effect
on SOD activity. However, evidence from further randomized controlled trials is required
to confirm the potential antioxidant effect of statin therapy.
Diagnosis and Management of Riga-fede Disease in a Healthy 7-month-old Child: A Case Report
Authors: Ritwik Priyanshi 
Published: September 2023

Background:
Riga-Fede disease (RFD) presents as oral mucosal ulceration in anatomical
juxtaposition to sharp dental surfaces.

Case Presentation:
A 7-month-old healthy male presented to the pediatric dentist for an emergency
appointment. His mother was concerned about episodic, recurrent, and excessive bleeding from
his mouth for three weeks. She reported that her son would often wake up in “a pool of blood”.
The pediatrician assessed the patient when oral bleeding first started and prescribed magic mouthwash.
This did not resolve the problem. The mother then took the child to an urgent care clinic,
where she was referred to a pediatric dentist. The pediatric dentist noted a sublingual traumatic,
granulomatous ulcer on the ventral surface of the tongue, extending up to the tip of the tongue. The
mandibular primary central incisors had recently erupted. The pediatric dentist established a clinical
diagnosis of RFD. Incisal edges were smoothened with dental polishing tips on a slow-speed
handpiece and topical fluoride varnish was applied to the teeth. The size of the ulceration decreased
over two weeks. However, in three weeks, there was another bleeding episode. The pediatric
dentist smoothened the incisal edges again, followed by a fluoride varnish application. Eight
weeks after the initial dental intervention, the lesion resolved completely without further bleeding.

Discussion:
The details of this case report highlight the maternal concerns, interprofessional educational
touchpoints, scope for interprofessional collaborative care, and noninvasive management of
Riga-Fede disease.

Conclusion:
In neurotypical patients, conservative dental management by smoothening sharp dental
surfaces can resolve RFD. RFD should be differentiated from viral illnesses which present with
oral ulcerations.
Psychometric Properties of the Antenatal Psychosocial Stress Scale in
Turkish Pregnant Women
Authors: Aypar Akbag Nuran Nur , Sanli Yasemin , Göke Arslan Gamze 
Published: September 2023

Background:
Minimizing the stress levels of pregnant women is important to giving
healthy birth and raising healthy generations. Therefore, there is a need for effective methods to diagnose
stress early in pregnancy.

Objective:
The purpose of this research was to examine the psychometric features of the Antenatal
Psychosocial Stress Scale (APSS) in pregnant Turkish women.

Methods:
This methodological study was conducted on 290 pregnant women.

Results:
The Turkish version of the APSS based on Exploratory Factor Analysis was found to have
70.215 percent of the total variance. It had a Cronbach's alpha coefficient of 0.78. After the confirmatory
factor analysis, the following were determined: χ2/df: 1.663, CFI: 0.978, RMSEA: 0.048, GFI: 0.
972.

Conclusion:
The Turkish sample found the APSS to be a valid and trustworthy assessment instrument
for pregnant women.
Novel “HYDALJSS08” Hydroalcoholic Polyherbal Formulation Development and Ultra-performance Liquid Chromatographic Separation,
Estimation of Andrographolides in Andrographis paniculata whole Plants
and a Marketed Siddha-based Polyherbal Formulation “Kabusura Kudineer”
Authors: Jatoth Ramkishan , S.P Dhanabal , Senthil Venkatachalam , Ganesh Thangavel , Selvaraj Jubie , M.R. Jeyprakash. , Basavan Duraiswamy 
Published: September 2023

Background:
The Siddha-based polyherbal formulation known as “Kabusura Kudineer (Marketed)"
and developed as “HYDALJSS08” hydroalcoholic polyherbal formulation contains some fifteen
plant materials in a dried raw form. Due to its immuno-booster properties, the Ministry of Ayush, Govt of
India, highly recommended the use of "Kabusura Kudineer" during the pandemic of COVID-19.

Objective:
The present study intends to expand and validate the analytical profile for Andrographolides
(AP), and isolated Andrographolides (AP) from the Andrographis Paniculata whole plant
and in the Polyherbal Formulations (Marketed-Kabusura Kudineer, & Developed “HYDALJSS08”).

Methods:
One of the active components of “Kabusura Kudineer” marketed and developed as
“HYDALJSS08” Hydroalcoholic Polyherbal formulation is kalmegh, also known as the king of bitter
(Andrographis Paniculata-Acanthaceae). Kalmegh composes active principal components of Andrographolides
(AP), which are proven for their Anti-viral and immunomodulatory activity. The preliminary
identification of AP and the sample was carried out by TLC and FT-IR. The liquid chromatography
was performed on a Zorbaz SB C8 (250*4.6mm & 5μm). The mobile phase incorporated pH 2.8
phosphate buffer with Acetonitrile: Methanol (60:30:10). The flow rate of the mobile phase was
1ml/min, and effluents were kept an eye on at 223 nm in a UV detector. The run time on the chromatogram
was 10 min, and retention time was also observed.

Results:
The Rf value of Andrographolides (AP) was found to be 0.62. ICH guidelines were followed
to carry out the Validation parameter. The retention time of AP was 2.5 min, and the Valid parameters of
AP and system precision were as follows: SD (1831.11), % RSD (0.2), regression equations y = 41978 +
x−10763, and correlation coefficient (R2) 0.9994. The adequate Linearity concentration was found to be
5 to 50 μg/ml, the value of LODs was 0.61μg /ml, LOQs was 2.01 μg/ml, method precision % RSD was
0.2, SD was 1597.1, and recovery was 99.9% and 101%. AP content found in a formulation (“Kabusura
Kudineer” 1.48 μg/mL, developed “HYDALJSS08” Hydroalcoholic Polyherbal formulation-0.48 μg/ml)
and isolated Andrographolides from Andrographis paniculata was 112.4μg/ml.

Conclusion:
The developed HPLC methods enabled simple, novel, rapid, easy, accurate, reproducible,
and linear analysis of isolated andrographolides, and Siddha-based Polyherbal formulations.
DESKRIPSI TAMPILAN PRODUKSI, KONSUMSI DAN KUALITAS SUSU SAPI PERAH FRIES HOLLAND (FH) di KUBE PSP MAJU MAPAN
Authors: Bai Muhammad Amrizal 
Published: September 2023

Sapi perah adalah salah satu hewan mamalia yang dapat memproduksi susu. Susu sapi perah dapat menyuplai kebutuhan susu di masyarakat karena susu sapi perah masih banyak diminati oleh masyarakat umum. Formula pakan atau konsentrat akan mempengaruhi produksi dan kualitas susu. Konsentrat harus memenuhi keseimbangan kadar protein, Total Digestible Nutrient (TDN), dan National Research Council (NRC) yang dibutuhkan oleh sapi perah laktasi. Sapi perah laktasi membutuhkan konsentrat untuk memenuhi kebutuhan hidup pokok, produksi dan reproduksi. Tujuan penelitian ini adalah untuk mengetahui gambaran produksi, konsumsi, dan kualitas susu sapi Fries Holland (FH) dengan pemberian pakan konsentrat yang berbeda. Penelitian ini menggunakan materi sapi perah laktasi 1 – 5 milik anggota Kube PSP Maju Mapan yang berjumlah 8 orang dengan kepemilikan 1 – 3 ekor. Metode yang digunakan pada penelitian ini adalah metode survei dengan observasi langsung kepada 8 peternak guna mengetahui jumlah konsumsi hijauan, konsentrat, produksi susu dan pemberian formula pakan yang berbeda antara sapi a dan b yang diberikan pada ternak sapi perah FH. Data yang diperoleh dianalisis menggunakan deskriptif kuantitatif guna mengungkapkan hasil penelitian berdasarkan data. Hasil penelitian ini menunjukkan bahwa pemberian pakan konsentrat yang berbeda pada sapi b dengan penambahan komposisi bungkil kopra impor dapat menigkatkan nilai protein sebesar 0,69% sehingga meningkatkan volume susu pagi 0,41 liter dan pada sore hari sebesar 0,66 liter per hari per ekor
Propiedades psicométricas de la Escala de Medida del Estado Actual (MOCS) en pacientes mexicanos con cáncer
Authors: Espinoza Bello Marcos , Galindo Vázquez Oscar , Penedo Frank J. , Jiménez Ríos Miguel Ángel , Ortega Andeane Patricia , Scavuzzo Anna , García Méndez Mirna , Meneses-García Abelardo 
Published: September 2023

Antecedentes: El cáncer es un problema de salud pública, una de las variables psicosociales en relación con esta enfermedad es el estrés, por tanto es necesario tener una escala que mida el estrés en población hispana con cáncer. Objetivo: Determinar las propiedades psicométricas de la Escala de Medida del Estado Actual (MOCS, por sus igla en inglés) en pacientes mexicanos con cáncer. Método: Estudio transversal, instrumental no experimental. Participaron 197 pacientes de 18 a 81 años, de cualquier etapa clínica, en tratamiento o seguimiento del cáncer. Instrumentos: MOCS y termómetro de malestar emocional. Análisis estadísticos: análisis factorial confirmatorio para evaluar el ajuste del modelo a los datos mediante el método de máxima verosimilitud y puntos de corte. Resultados: Se obtuvieron dos modelos, el primero, estado actual autopercibido, con un alfa de .90 varianza explicada de 74.2 %, el segundo, efectos inespecíficos, con un alfa de .61 varianza explicada de 74.3 %. El análisis factorial confirmatorio mostró que ambos modelos tuvieron índices que sugieren estabilidad, ajuste aceptable, se muestran puntajes mediante rangos intercuartílicos para cada modelo. Conclusiones: la versión mexicana del MOCS tiene confiabilidad y validez, su estructura factorial confirmatoria es similar a la versión original. Se recomienda su uso en la práctica clínica e investigación en pacientes con cáncer.
Predicted overbite and overjet changes with the Invisalign appliance: a validation study
Authors: Meade Maurice J. , Blundell Haylea , Weir Tony 
Published: September 2023

ABSTRACT

Objectives
To investigate whether the predicted changes in overbite (OB) and overjet (OJ) in Align Technology’s (Santa Jose, Calif) digital treatment plan for the prescribed course of treatment with the Invisalign appliance were consistent with those same changes in OB and OJ measured with the Geomagic Control X (Geomagic US, Research, Triangle Park, NC) metrology software system.

Materials and Methods
Geomagic Control X software was used to determine OB and OJ differences between the pretreatment and predicted outcome. STL files for patients satisfying inclusion criteria and undergoing nonextraction orthodontic treatment with the Invisalign appliance. The differences were compared, using the Bland-Altman analysis, to the corresponding data provided by Align Technology’s digital treatment-planning interface, ClinCheck.

Results
Data regarding 76 adult patients who satisfied strict inclusion criteria were assessed. The Shapiro-Wilks test indicated normality (P &gt; .05). The Bland-Altman analysis showed high levels of agreement between the two measurements, with a bias range of −0.131 to 0.111 for OB and −0.393 to 0.03 for OJ recorded.

Conclusions
Clinicians and researchers can be confident that measurement data provided by Invisalign’s ClinCheck digital treatment-planning facility concerning OB and OJ changes from the initial to the predicted treatment outcome are valid.
State-legal mechanism for ensuring freedom of entrepreneurship in the conditions of martial law
Authors: Darahanova N. 
Published: September 2023

The article examines the state-legal mechanism for ensuring freedom of entrepreneurial activity in the conditions of the legal regime of martial law, as well as analyzes the measures necessary to ensure such freedom.It was established that the freedom of entrepreneurial activity does not belong to the absolute rights of a person, therefore it can be subject to certain legally defined restrictions, especially during the operation of the legal regime of martial law.
It was found that the definition of general temporal, substantive «permits» and «prohibitions» in the sphere of ensuring freedom of entrepreneurial activity at the national level both in peacetime and in wartime is carried out by legal acts of the head of state, parliament, government and central executive bodies, instead «сoncretization» of these «permits» and «prohibitions» in the sphere of entrepreneurship development, as well as its practical support, is carried out by the grassroots vertical of state executive authorities, local self- government bodies and their officials, in particular, by means of their adoption of certain regulatory acts. It was concluded that the state of affairs for ensuring the freedom of entrepreneurship in Ukraine during the legal regime of martial law worsened, compared to peacetime. The reason for this is not only war or gaps or low quality of legal regulation of these relations, but mostly corruption and improper law enforcement practices on the part of public authorities and their officials.A system of steps necessary to ensure the freedom of entrepreneurial activity and to optimize the functionality of the state-legal mechanism in Ukraine in this segment of economic relations is proposed.There are such steps: effective and systematic destruction of corruption; maximum minimization of subject composition and managerial influence in relations related to licensing and documentary support of entrepreneurial activity; autonomous and equidistant from the government, ministries, and other public authorities, independent systematic monitoring of the expenditure of budget funds, investment and other financial resources directed to the sphere of entrepreneurial activity, with the direct participation of public activists and foreign partners; increased responsibility for corrupt activity or any involvement in it; strengthening the requirements for the formation of the judicial corps and its responsibility.