bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efferocytosis is restricted by axon guidance molecule EphA4 via ERK/Stat6/Mertk signaling following brain injury
Authors: Soliman Eman , Leonard John , Basso Erwin Kristobal , Gershenson Ilana , Ju Jing , Mills Jatia , Jager Caroline , Kaloss Alexandra M. , Elhassanny Mohamed , Pereira Daniela , Chen Michael , Wang Xia , Theus Michelle H. 
Published: June 2023

Abstract
Background
Efferocytosis is a process that removes apoptotic cells and cellular debris. Clearance of these cells alleviates neuroinflammation and prevents the release of inflammatory molecules and promotes the production of anti-inflammatory cytokines to help maintain tissue homeostasis. The underlying mechanisms by which this occurs in the brain after injury remains ill-defined.
Methods
We demonstrate using GFP bone marrow chimeric knockout (KO) mice, that the axon guidance molecule EphA4 receptor tyrosine kinase is involved in suppressing Mertk signaling in the brain to restrict the function of efferocytosis on resident microglia and peripheral-derived monocyte/macrophages.
Results
Single-cell RNAseq identified Mertk expression, the primary receptor involved in efferocytosis, on monocytes, microglia, and a subset of astrocytes in the damaged cortex following brain injury. Loss of EphA4 on infiltrating GFP-expressing immune cells improved functional outcome concomitant with enhanced efferocytosis, and overall protein expression of p-Mertk, p-ERK, and p-Stat6. The percentage of GFP+ monocyte/macrophages and resident microglia engulfing NeuN+ or TUNEL+ cells was significantly higher in KO chimeric mice. Importantly, mRNA expression of Mertk and its cognate ligand Gas6 was significantly elevated in these mice compared to wild-type. Analysis of cell-specific expression showed that p-ERK and p-Stat6 co-localized with Mertk-expressing GFP + cells in the peri-lesional area of the cortex following brain injury. Using an in vitro efferocytosis assay, co-culturing pHrodo-labeled apoptotic Jurkat cells and bone marrow (BM)-derived macrophages, we demonstrate that efferocytosis efficiency and mRNA expression of Mertk and Gas6 was enhanced in the absence of EphA4. Select inhibitors of ERK and Stat6 attenuated this effect confirming that EphA4 suppresses monocyte/macrophage efferocytosis via inhibition of the ERK/Stat6 pathway.
Conclusions
Our findings implicate the Mertk/ERK/Stat6 axis as a novel regulator of apoptotic debris clearance in brain injury that is restricted by peripheral myeloid-derived EphA4 to prevent the resolution of inflammation.
An Injectable and Thermosensitive Hydrogel with Nano-aided Phototherapeutic and Chemical Effects for Periodontal Antibacteria and Bone Regeneration
Authors: Wang Weixiang , Zhang Guorong , Wang Yanyi , Ran Jianchuan , Chen Lin , Wei Zheng , Zou Huihui , Cai Yu , Han Wei 
Published: June 2023

Abstract
Periodontitis is a common public health problem worldwide and an inflammatory disease caused by periodontal pathogens. Porphyromonas gingivalis (P. gingivalis) is the most dominant bacteria in periodontitis and cause irregular defect of periodontal tissue. Both antibacterial therapy and bone regeneration are of great importance in the treatment of periodontitis. In this study, injectable and thermosensitive hydrogels with 3D networks were used as carriers for controlled release of osteo-inductive agent (BMP-2) and Near Infrared Region-II (NIR-II) phototherapy agents (T8IC nano-particles). T8IC nano-particles were prepared by reprecipitation and acted as photosensitizer and crosslinkers of the hydrogel under 808 nm laser irradiation. Besides, we promoted photodynamic therapy (PDT) through adding H2O2 to generate more radical oxygen singlet (ROS) and facilitate the antibacterial effect instead of increasing the temperature of photothermal therapy (PTT). Hydrogel + T8IC + Laser + BMP-2 + H2O2 incorporated with mild PTT (45°C), enhanced PDT and sustained release of BMP-2. It was present with excellent bactericidal effect, osteogenic induction and biosafety in vitro and in vivo. Besides, it was confirmed that PTT and PDT could promote bone regeneration through alleviating inflammation state. Altogether, this novel approach with synergistic antibacterial effect, anti-inflammation and bone regeneration has a great potential for the treatment of periodontitis in the future.
CNN-HT: A Two-Stage Algorithm Selection Framework
Authors: Xu Siyi , Liu Wenwen , Wu Chengpei , Li Junli 
Published: June 2023

Abstract
The No Free Lunch theorem tells us that no algorithm can beat other algorithms on all types of problems. The algorithm selection structure is proposed to select the most suitable algorithm from a set of algorithms for an unknown optimization problem. In this paper, an innovative algorithm selection approach called the CNN-HT two-stage algorithm selection framework is introduced. In the first stage, a Convolutional Neural Network (CNN) is employed to classify problems. In the second stage, the Hypothesis Testing (HT) technique is used to suggest the best-performing algorithm based on the statistical analysis of the performance metric of algorithms that address various problem categories. To provide a more general structure for the classification model, we adopt Exploratory Landscape Analysis (ELA) features of the problem as the input and utilize feature selection techniques to reduce the redundant ones. In problem classification, our proposed algorithm selection framework achieves an average accuracy of 96% for the classification of unknown black-box problems, and it improves to 98.8% after feature selection. This shows that our proposed classification method can accurately classify BBOB problems into 24 classes of problems and thus can correctly recommend algorithms accordingly. The performance of CNN-HT is compared with a single optimization algorithm on a continuous black-box optimization problem set, and the average ranking of CNN-HT is superior, demonstrating its effectiveness.
Saccharum officinarum Bio-coagulant for Palm Oil Mill Effluent Treatment
Authors: Bahrodin Muhammad Burhanuddin , Zaidi Nur Syamimi , Hussein Norelyza , Zhan Loh Zhang , Silanpää Mika , Kadier Abudukeremu 
Published: June 2023

Abstract
The coagulation process has become a common treatment method in water and wastewater treatment but not in palm oil mill effluent (POME) treatment. POME is known to have a high level of COD and turbidity and prior studies showed that the modified coagulation process using bio-coagulant may be feasible to remove primarily the COD and turbidity of the POME. In this study, an extract of Saccharum officinarum or bagasse is used as a source of bio-coagulant for the removal of COD and turbidity from POME at optimized coagulant dosage, pH, and contact time. The optimization was carried out via response surface analysis. This analysis stipulated that the application of Saccharum officinarum extract removed turbidity of more than 95%, COD of more than 65.0% with coagulation activity of 67.4% at optimum 50 mg/L coagulant dosage, pH 8, and slow mixing for 30 minutes. Henceforth, this study concludes the suitability of Saccharum officinarum as a bio-coagulant to primarily remove organic and solid pollutants during POME pre-treatment.
In vitro anthelminthic activity and secondary metabolites analysis of leaves of Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) against bovine parasite Onchocerca ochengi and drug resistant strains of the free-living nematode Caenorhabditis elegans
Authors: Kalmobé Justin , Vildina Jacqueline Dikti , Boursou Djafsia , Menga Honoré Ndouwé Tissebé , Fogue Simeon Kouam , Ndjonka Dieudonné 
Published: June 2023

Abstract

Background
Human onchocerciasis caused by the filarial worm, Onchocerca volvulus is a parasitic that forms nodules under the skin. In the developing countries, it has been estimated that more than 80% of the population rely on traditional medicines for their primary healthcare needs. The aim of this work was to assess the nematicidal activities of Aloe vera on Onchocerca ochengi and Caenorahbditis elegans and to determine the phytochemical compounds.
Methods
Nodules were collected from the umbilical region of infected cattle, dissected and male worms were cultured in RPMI-1640. Worms were incubated for 48 h and 72 h with different concentrations of A. vera extracts in RPMI-1640 and M9-buffer. Polyphenol, tannin and flavonoid contents of extract were determined by using gallic acid and rutin as standards. The antioxidant activity was evaluated by DPPH radical scavenging method.
Results
The anthelmintic effect of A. vera extract against O. ochengi was concentration dependent with LC50 of 20.71 µg/mL and 11.75 µg/mL after 48 and 72h respectively. A. vera extract exerted concentration dependent lethal effects (LC50 = 2747 and LC50 = 1937 µg/mL) against C. elegans (WT). MeOH-CH2Cl2 of A. vera extract exhibited high DPPH activity with an IC50 value of 15 µg/mL and 9 µg/mL for ascorbic acid. The highest activity in adult worms was observed with the MeOH (100: 0) and AcOEtMeOH fractions with LC50 values of 12.82 and 15.50 µg/mL respectively. EcOEtMeOH (8:2 v/v) was more effective (LC50 = 250 µg/mL) on WT C. elegans. A. vera contains polyphenols (1015.05 and AcOEtMeOH = 893.60), flavonoids (25.35 and MeOH = 225.76) and tannins (401.37 and Hex = 788.89).
Conclusions
A. vera showed in vitro nematicidal activity against O. ochengi and C. elegans. Aloe vera could be used as alternative anthelmintic for onchocerciasis treatment.
Network based on epoch-to-epoch adaptation weighted triplet loss for person re-identification
Authors: Wang Jiajing , Yang Mingqiang , Wu Bin 
Published: June 2023

Abstract
Person re-identification (Re-ID) aims to use computer vision techniques to determine whether a specific person is present in a set of images. With the widespread use of deep learning, how to efficiently make the deep neural network for person Re-ID achieve excellent performance has gained wide attention. In this paper, we propose a metric learning method based on a new Epoch-to-epoch Adaptation Weighted (EAW) triplet loss function. The EAW triplet loss function uses the variability and connectivity of metric information between epochs to guide the optimization direction of the network. It enhances the inter-class differentiation through the adaptive weight and margin, speeds up the convergence of the network, and improves accuracy without increasing cost. Meanwhile, to prevent the risk of overfitting due to the complex loss function, we regularly employ sample pairing to optimize the network. We conduct evaluation experiments on both Market-1501 and DukeMTMC-reID datasets. With the same network, our loss function can effectively improve the network performance. On the Market-1501, our method achieves 95.3% rank-1 accuracy and 89.2% mAP and on the DukeMTMC-reID, the mAP and rank-1 accuracy can reach 90.4% and 80.4% respectively. The experiments show that our method can effectively improve the accuracy and training efficiency.
Advantages of 99mTc-CNDG SPECT/CT over Enhanced CT in the Staging of Non-Small Cell Lung Cancer
Authors: Wang Qinfen , Deng Zhensheng , Lu Chuangang , Chen Lijun , Qin Jiangjun , Wang Ping 
Published: June 2023

Abstract

Objective To explore the value of 99mTc-isonitrile deoxyglucosamine (CNDG) SPECT/CT in the staging and resectability diagnosis of non-small cell lung cancer (NSCLC) compared with enhanced CT.
Methods This research was approved by the hospital ethics review committee. Sixty-three patients with NSCLC received 99mTc-CNDG SPECT/CT, enhanced CT and initial TNM staging before treatment. Thirty-three patients who underwent radical surgery took postoperative pathological TNM staging as the reference standard.Another thirty patients who underwent radiochemotherapy,among them the reference standard of 7 patients of N staging and 5 patients of M staging was based on biopsy pathology，and the diagnosis of the remaining lesions was confirmed by at least one different image or clinical imaging follow-up for more than 3 months. The McNemar test and receiver operating characteristic (ROC) curve analysis were used to compare the diagnostic accuracy of staging and resectability of 99mTc-CNDG SPECT/CT and enhanced CT in NSCLC，respectively.
Results For all patients and surgical patients, the accuracies of 99mTc-CNDG SPECT/CT in diagnosing the T stage and N stage were higher than those of enhanced CT (all patients: 90.5%, 88.9% vs. 79.4%, 60.3%; surgical patients: 81.8%, 78.8% vs. 60.6%, 51.5%),and the differences were statistically significant (all patients: T stage, P=0.016; N stage, P =0.000; surgical patients: T stage, P =0.016; N stage, P =0.004). For all patients，the accuracy of 99mTc-CNDG SPECT/CT in diagnosing the M stage was higher than that of enhanced CT(96.8% vs. 90.5%), but the difference was not statistically significant (P =0.289).ROC curve analysis showed that the accuracy of 99mTc-CNDG SPECT/CT in diagnosing the potential resectability of NSCLC was significantly better than that of enhanced CT(P=0.046).
Conclusion This preliminary clinical study shows that 99mTc-CNDG SPECT/CT is of great value for accurate clinical staging of NSCLC compared with enhanced CT, and can significantly improve the accuracy of resectability diagnosis.
Habitat and population structure determine patterns of plant-pollinator networks of an endangered palm tree in a grassland-forest ecotone
Authors: Raguse-Quadros Mateus , Souza Gabriela da Cunha , Ferreira Pedro Maria Abreu , Blochtein Betina 
Published: June 2023

Abstract

Pollen transport by insects determines patterns of reproductive encounters between plants with flowers that have spatially or temporally segregated sexes. Pollinators show varied responses to environmental gradients such as those found in grassland-forest ecotones. Individual-based interaction networks are useful yet underexplored tools to understand how interactions vary across these gradients. Interactions between plant individuals and their pollinators directly reflect on plants fitness and genetic structure, seminal attributes for the conservation of endangered species. To test how a grassland-forest ecotone gradient can affect these interactions we studied pollination networks of Butia odorata individuals, an extinction-threatened palm tree from remnant palm grove ecosystems in South America. We evaluated how network metrics (specialization and modularity), and pollinator richness respond to gradients of habitat and population structure in a grassland-forest ecotone. Networks with more isolated palm trees showed greater specialization and modularity. Pollinator richness was dependent on the habitat context and pollinator role: peripheral pollinators were negatively affected by palm density, whereas core pollinators were positively affected by tree cover, which in turn was positively associated with palm density and proximity to the forest. Our results indicated that increased tree cover in the grassland matrix can promote pollinator diversity by decreasing the dominance of core species. Palm density may hamper the movement of pollinators pollen transportation, playing a key role for the conservation of B. odorataand for palm grove ecosystems. Finally, we emphasize the need of protocols that include traditional grassland management to achieve tree and palm tree density that maximizes conservation results.
A family of fractional analytic polynomials in generalized complex numbers and applications
Authors: Teodoro Antonio Di , Morillo Esteban , Ochoa--Tocachi Diego R. , Palacios Jose , Vela Erick 
Published: June 2023

Abstract
We present the development of fractional generalized complex calculus in the Riemann-Liouville sense, modifying the Cauchy-Riemann operator using the one-dimensional Riemann-Liouville derivative. We analyze the properties of the correspondent fractional analytic functions and introduce a family of analytic polynomials which are necessary to construct series expansions. We provide examples and visuals of these polynomials and use them to solve fractional differential equations and verify fractional Leibniz rule numerically.
Place-based Policy, Government Intervention and Public Service: Evidence from “The Construction of Third Front”
Authors: Zhang Haotian , Lu Shengfeng 
Published: June 2023

Abstract
This study examines the long-term effects of a significant place-based policy in China's history- “The Construction of Third Front”, which led to the relocation of significant amounts of industry and labor from coastal to inland regions under the central government's control. Using a spatially discontinuous design, we identify that the policy has resulted in a hindrance to the development public services in the affected regions over time, with the gap between these and the eastern regions continuing to widen. We argue that the overzealous intervention of the central government disrupted the efficiency of factor allocation in the TF region and caused a significant impact on supply and demand patterns for public services in this area, which persists even after the cessation of the policy.
JEL codes:H41, H50, O25
Endpoint Estimates for Commutators of Fractional Integral Operators on the p-Adic Vector Space
Authors: Chang Yunpeng , Wu Jianglong 
Published: June 2023

Abstract
In this paper, the main aim is to prove the weak type L log L estimates for commutators of fractional integral operators and the higher order in the context of the p-adic version of Lebesgue spaces, where the symbols of the commutators belong to the p-adic version of BMO space, in addition, we also establish the estimates of the sharp function on the p-adic vector space.
AMS(2020) Subject Classification: 42B35, 11E95, 26A33, 26D10
Variability of caffeine metabolism by CYP1A2 polymorphism in different populations
Authors: Chiarella Pieranna , Capone Pasquale , Sisto Renata 
Published: June 2023

Abstract
In 2015, the European Food and Safety Authority (EFSA) evaluated the worldwide consumption of coffee to verify the potential use and abuse of caffeine by the population, with the aim to identify potential adverse effects on the human health. The conclusion of the survey was the following: single doses of caffeine up to 200/400 mg did not give to any concern in the population.
Objectives: In 2015, the European Food and Safety Authority (EFSA) evaluated the worldwide consumption of coffee to verify the potential use and abuse of caffeine by the population, with the aim to identify potential adverse effects on the human health. The conclusion of the survey was the following: single doses of caffeine up to 200/400 mg wer not dangerous for the consumers.
Methods: Caffeine is one of the most widely consumed substance and beverage in the world, showing not only benefits, as excellent source of antioxidants, but also offering to prevent inflammatory and oxidative stress-related diseases, including obesity, metabolic syndrome and type 2 diabetes. In the elderly people with several comorbidities, caffeine contributed to reduce several neurological disorders, such as senile dementia, Alzheimer’s and Parkinson’s disease, contributing to alleviate tremors and helping the memory loss in elderly subjects.
Results: It is widely known CYP1A2 polymorphic enzyme (AA) (AC) (CC) is responsible for different levels in the caffeine metabolism, leading to a distinction in separate categories where CYP1A2*1A allele (AA) are "rapid" caffeine metabolizers, in contrast to carriers of the variant CYP1A2*1F who are "slow" caffeine metabolizers (AC-CC). In the absence of biological matrix, such as blood and urine, we performed in silicoanalysis of the genetic polymorphism CYP1A2*1A rs762551 distributed into five different ethnic groups of 210 subjects, including Caucasian, Africans, Americans, South Asians and East Asians.
Conclusions: The goal of this study is to identify potential significant difference in metabolism of caffeine to verify the most susceptible individuals in five ethnic groups.
Structured abstract
Coffee is one of the most widely consumed beverage in the world although it is not free from various effects and in 2015 the exposure to caffeine and the intake of daily coffee has been regulated by EFSA. Guidelines and EFSA results demonstrated caffeine is easily metabolized in the majority of subjects, although fast metabolizers are advantaged in contrast to other individuals with a slow metabolism.
Anti-inflammatory Effects of Boric Acid in Treating Knee Osteoarthritis: Biochemical and Histopathological Evaluation in Rat Models
Authors: Gundogdu Koksal , Gundogdu Gulsah , Miloglu Fatma Demirkaya , Demirci Tuba , Tascı Seymanur Yılmaz , El-Aty A. M. Abd 
Published: June 2023

Abstract
Objective: This study aimed to examine the anti-inflammatory properties of boric acid(BA) in treatingknee osteoarthritis(KOA) in rats, evaluating its biochemical and histopathological therapeutic effects.
Methods: The KOA rat model was induced by injecting monosodium iodoacetate into the knee joint. Random assignment was performed for the experimental groups as follows: group-1(control), group-2(KOA control), group-3(BA:4 mg/kg,orally), group-4(BA:10 mg/kg,orally), group-5 (BA:4 mg/kg,intra-articularly), and group-6(BA:10 mg/kg,intra-articularly). The rats received 100 µL of BA intra-articularly on days 1,7,14, and 21 or 1 mL orally once a day (5 days/week) for 4 weeks. Serum levels of interleukin-1β (IL-1β), tumor necrosis factor-α(TNF-α), and matrix metalloproteinase-13(MMP-13) were measured. Histopathological and immunohistochemical analyses were performed on knee joint samples using specific antibodies for IL-1β, TNF-α, MMP13, and nitric oxide synthase-2(NOS-2).
Results. Group-2 exhibited higher serum levels of IL-1β, TNF-α, and MMP-13 than group-1(P<0.05). However, these levels were lower in all treatment groups compared to group-2, with statistically significant reductions observed in groups-4,5, and 6. Histopathologically, group-2 displayed joint space narrowing, cartilage degeneration, and deep fissures. Groups-5 and 6 demonstrated significant joint space enlargement, articular cartilage tissue regeneration, and immunostaining patterns similar to those in group-1. Immunohistochemically, group-2 showed significant increases in IL-1β, TNF-α, MMP-13, and NOS-2 expression. However, all treatment groups exhibited reductions in these expression levelscompared to group-2, with statistically significant decreases observed in groups-5 and 6(P<0.01).
Conclusions. BA shows potential efficacy in reducing inflammation in experimental KOA models in rats. It may be a promising therapeutic agent for KOA, warranting further clinical studies for validation.
Synthesis of Nb2O5/C for H2O2 electrogeneration and its application for the degradation of levofloxacin
Authors: Valim Ricardo Bertholo , Carneiro Jussara Fernandes , Lourenço Julio César , Hammer Peter , Santos Mauro Coelho , Rodrigues Liana Alvares , Bertazzoli Rodnei , Lanza Marcos Roberto Vasconcelos , Rocha Robson da Silva 
Published: June 2023

Abstract
The present work sought to investigate H2O2 generation using a gas diffusion electrode composed of carbon with niobium oxide for the degradation of levofloxacin. Results showed that the oxide formed was Nb2O5 (crystallographic structure was confirmed by XRD; chemical composition detected by XPS) and the increase in the Nb/C ratio led to more crystalline structure and higher crystallite size. The carbon with 5% Nb2O5 presented an average particle size of 5.6 nm and this material led to the generation of the highest amount of H2O2 (317.6 mg L− 1). The C/5% Nb2O5-modified GDE was applied for the degradation of levofloxacin in an acid medium, in the presence and absence of Fe2+ ions. Long-term experiments conducted for the analysis of the time required for the total removal of levofloxacin showed that 96% of levofloxacin and 66% of organic load were removed in 270 min of treatment at the current density of 100 mA cm− 2.