bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rumen microbiome response to sustained release mineral bolus supplement with low- and high-quality forages
Authors: Eberly Jed O. , Wyffels Samuel A. , Carlisle Tanner J. , DelCurto Timothy 
Published: June 2023

IntroductionLimited forage quantity and quality are challenges faced in livestock production systems in semi-arid rangelands of the western United States, particularly when livestock face stressors such as cold weather or have increased nutritional requirements such as during pregnancy and lactation. To meet livestock nutrient requirements, producers frequently provide supplemental nutrition, however there is limited knowledge regarding the effects of these practices on the rumen microbiome in these environments.MethodsA study was conducted to evaluate changes in the rumen microbiome in response to high- and low- quality forage with sustained release mineral boluses. The study consisted of 16 ruminally-cannulated 2–3-year-old black angus cows fed high quality grass alfalfa hay or low-quality grass hay with a 90 or 180 day sustained release mineral bolus. Rumen samples were collected pre-feeding and 8 hours post feeding and bacterial 16S rRNA gene amplicons were sequenced from the rumen fluid.ResultsAlpha diversity as measured by Shannon’s diversity index decreased significantly over time (p&lt;0.01) and averaged 5.6 pre-feeding and 5.4 post- feeding and was not significantly different between high- and low-quality forages or between mineral bolus types (p&gt;0.05). Principal coordinates analysis (PCoA) of the Bray-Curtis dissimilarity matrix showed distinct grouping by feed quality and time but not by mineral bolus type. Bacteroidetes and Firmicutes were the dominant phyla in all treatments and significant increases (p&lt;0.05) in the relative abundance of the family Lachnospiraceae and the genus Prevotella were observed in high quality forage diets. Rumen VFA and NH3-N concentrations were also strongly associated with the high-quality forage diet. Predictive functional profiling indicated that functions associated with methanogenesis were negatively correlated with feed quality.DiscussionThe results of this study suggest that mineral bolus type is unlikely to affect rumen bacterial community structure or function while forage quality can significantly alter community structure and predicted functions associated with methanogenesis and VFA production.
Lipoaspirate fluid derived factors and extracellular vesicles accelerate wound healing in a rat burn model
Authors: Wu Yue , Hong Pengyu , Liu Pan , Zhang Qi , Zhang Yue , Yang Baohua , Liu Huixing , Liu Lei , Tian Weidong , Yu Mei 
Published: June 2023

Background: The regenerative capabilities of derivatives derived from the fat layer of lipoaspirate have been demonstrated. However, the large volume of lipoaspirate fluid has not attracted extensive attention in clinical applications. In this study, we aimed to isolate the factors and extracellular vesicles from human lipoaspirate fluid and evaluate their potential therapeutic efficacy.Methods: Lipoaspirate fluid derived factors and extracellular vesicles (LF-FVs) were prepared from human lipoaspirate and characterized by nanoparticle tracking analysis, size-exclusion chromatography and adipokine antibody arrays. The therapeutic potential of LF-FVs was evaluated on fibroblasts in vitro and rat burn model in vivo. Wound healing process was recorded on days 2, 4, 8, 10, 12 and 16 post-treatment. The scar formation was analyzed by histology, immunofluorescent staining and scar-related gene expression at day 35 post-treatment.Results: The results of nanoparticle tracking analysis and size-exclusion chromatography indicated that LF-FVs were enriched with proteins and extracellular vesicles. Specific adipokines (adiponectin and IGF-1) were detected in LF-FVs. In vitro, LF-FVs augmented the proliferation and migration of fibroblasts in a dose-dependent manner. In vivo, the results showed that LF-FVs significantly accelerated burn wound healing. Moreover, LF-FVs improved the quality of wound healing, including regenerating cutaneous appendages (hair follicles and sebaceous glands) and decreasing scar formation in the healed skin.Conclusion: LF-FVs were successfully prepared from lipoaspirate liquid, which were cell-free and enriched with extracellular vesicles. Additionally, they were found to improve wound healing in a rat burn model, suggesting that LF-FVs could be potentially used for wound regeneration in clinical settings.
Out-of-plane behavior of innovative infill walls made of autoclaved aereated concrete (AAC) subjected to shake table tests
Authors: Giulivo Marco , Borretti Giovanbattista , Prota Andrea , Menna Costantino 
Published: June 2023

Introduction: Seismic damage affecting the non-structural components of buildings (such as infills) can significantly impact the expected economic losses over a structure’s service life. Recently, autoclaved aerated concrete (AAC) infills have gained attention in the construction industry due to their light weight, energy efficiency, and durability.Methods: This study investigates the out-of-plane behavior of an innovative AAC infill wall system by means of shake table tests. The wall system was made of low-density AAC units with innovative joints made from polyurethane resin and steel plates for connection to a structural frame. Shake table tests were carried out using artificial accelerograms based on ICBO-AC156, which can reproduce a wide range of interstory drift demands. The study is articulated in three stages: the mechanical characterization of the AAC infill wall, shake table tests, and seismic behavior analysis of the wall based on current code requirements.Results and Discussions: The results demonstrate the effectiveness of the innovative AAC infill wall system in resisting out-of-plane seismic loads, also considering different levels of seismic action, with base accelerations reaching up to 0.80 g.
Analysis of coexisting pathogens in nasopharyngeal swabs from COVID-19
Authors: Gao Zhan , Yu Lisong , Cao Ling , Yang Meng , Li Yuhui , Lan Yue , Tang Ruixiang , Huang Yang , Luan Guangxin , Liu Yingfen , Yu Hailin , Jian Ling , Zha Yi , Fan Zhenxin , Bai Yujiao , Luo Mei , He Miao , Deng Shanshan 
Published: June 2023

BackgroundThe impact of COVID-19 on the world is still ongoing, and it is currently under regular management. Although most infected people have flu-like symptoms and can cure themselves, coexisting pathogens in COVID-19 patients should not be taken lightly. The present study sought to investigate the coexisting pathogens in SARS-CoV-2 infected patients and identify the variety and abundance of dangerous microbes to guide treatment strategies with a better understanding of the untested factors.MethodsWe extracted total DNA and RNA in COVID-19 patient specimens from nasopharyngeal swabs to construct a metagenomic library and utilize Next Generation Sequencing (NGS) to discover chief bacteria, fungi, and viruses in the body of patients. High-throughput sequencing data from Illumina Hiseq 4000 were analyzed using Krona taxonomic methodology for species diversity.ResultsWe studied 56 samples to detect SARS-CoV-2 and other pathogens and analyzed the species diversity and community composition of these samples after sequencing. Our results showed some threatening pathogens such as Mycoplasma pneumoniae, Klebsiella pneumoniae, Streptococcus pneumoniae, and some previously reported pathogens. SARS-CoV-2 combined with bacterial infection is more common. The results of heat map analysis showed that the abundance of bacteria was mostly more than 1000 and that of viruses was generally less than 500. The pathogens most likely to cause SARS-CoV-2 coinfection or superinfection include Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Klebsiella pneumoniae, and Human gammaherpesvirus 4.ConclusionsThe current coinfection and superinfection status is not optimistic. Bacteria are the major threat group that increases the risk of complications and death in COVID-19 patients and attention should be paid to the use and control of antibiotics. Our study investigated the main types of respiratory pathogens prone to coexisting or superinfection in COVID-19 patients, which is valuable for identifying and treating SARS-CoV-2.
Cardiac magnetic resonance imaging for preprocedural planning of percutaneous left atrial appendage closure
Authors: Bertsche Dagmar , Metze Patrick , Luo Erfei , Dahme Tillman , Gonska Birgid , Rottbauer Wolfgang , Vernikouskaya Ina , Rasche Volker , Schneider Leonhard M. 
Published: June 2023

IntroductionPercutaneous closure of the left atrial appendage (LAA) facilitates stroke prevention in patients with atrial fibrillation. Optimal device selection and positioning are often challenging due to highly variable LAA shape and dimension and thus require accurate assessment of the respective anatomy. Transesophageal echocardiography (TEE) and x-ray fluoroscopy (XR) represent the gold standard imaging techniques. However, device underestimation has frequently been observed. Assessment based on 3-dimensional computer tomography (CTA) has been reported as more accurate but increases radiation and contrast agent burden. In this study, the use of non-contrast-enhanced cardiac magnetic resonance imaging (CMR) to support preprocedural planning for LAA closure (LAAc) was investigated.MethodsCMR was performed in thirteen patients prior to LAAc. Based on the 3-dimensional CMR image data, the dimensions of the LAA were quantified and optimal C-arm angulations were determined and compared to periprocedural data. Quantitative figures used for evaluation of the technique comprised the maximum diameter, the diameter derived from perimeter and the area of the landing zone of the LAA.ResultsPerimeter- and area-based diameters derived from preprocedural CMR showed excellent congruency compared to those measured periprocedurally by XR, whereas the respective maximum diameter resulted in significant overestimation (p &lt; 0.05). Compared to TEE assessment, CMR-derived diameters resulted in significantly larger dimensions (p &lt; 0.05). The deviation of the maximum diameter to the diameters measured by XR and TEE correlated well with the ovality of the LAA. C-arm angulations used during the procedures were in agreement with those determined by CMR in case of circular LAA.DiscussionThis small pilot study demonstrates the potential of non-contrast-enhanced CMR to support preprocedural planning of LAAc. Diameter measurements based on LAA area and perimeter correlated well with the actual device selection parameters. CMR-derived determination of landing zones facilitated accurate C-arm angulation for optimal device positioning.
Fibroblast growth factor 21 resistance is associated with body shape in patients with type 2 diabetes complicating hypertension
Authors: Gan Jing , Duan Zikun , Tang Lu , Liu Zhen , Tian Huiying , Wu Maolan , Bi Yanxue , Pan Xingchao , Wang Wenjun , Gao Xiaotang , Wang Ningrui , Lin Zhuofeng , Yang Hong 
Published: June 2023

ObjectivesObesity, especially abdominal obesity, increases the prevalence of metabolic and cardiovascular disease (CVD). Fibroblast growth factor 21 (FGF21) has been identified as a critical regulator playing a therapeutic role in diabetes and its complications. This study aims to evaluate the relationship between serum FGF21 levels and body shape parameters in patients with hypertension (HP) and type 2 diabetes mellitus (T2DM).MethodsSerum FGF21 levels were determined in 1,003 subjects, including 745 patients with T2DM, and 258 individuals were selected as a healthy control in this cross-sectional study.ResultsSerum FGF21 levels were significantly higher in T2DM patients with HP than those without [534.9 (322.6–722.2) vs. 220.65 (142.8–347.55) pg/ml, p &lt; 0.001], and levels in both of these two groups were significantly increased compared with that of healthy control [123.92 (67.23–219.32) pg/ml, all p &lt; 0.001]. These differences were also observed in body shape parameters, including weight, waistline, body mass index (BMI), body shape index (ABSI), and the percentage of abdominal obesity. Serum FGF21 levels in T2DM patients were positively correlated with body shape parameters, including weight, waistline, neck circumference, BMI, ABSI, percent of abdominal obesity, and triglyceride, while negatively with estimated glomerular filtration rate (all p &lt; 0.01). The significance remained stable when adjusted for age and T2DM duration. In addition, both serum FGF21 concentrations and waistline were independently associated with HP in T2DM patients after the adjustment for risk factors (all p &lt; 0.05). ROC analysis for FGF21 levels of 745 patients with T2DM identified 411.33 pg/ml as an optimal cut-off point to predict HP, with a sensitivity and specificity of 66.0% and 84.9%, respectively.ConclusionsFGF21 resistance occurs in patients of HP in T2DM, and positively correlates with body shape parameters (especially waistline and BMI). High levels of FGF21 may be a compensatory reaction to offset HP.
Cardiac contractility is a key factor in determining pulse pressure and its peripheral amplification
Authors: Piccioli Francesco , Li Ye , Valiani Alessandro , Caleffi Valerio , Chowienczyk Phil , Alastruey Jordi 
Published: June 2023

BackgroundArterial stiffening and peripheral wave reflections have been considered the major determinants of raised pulse pressure (PP) and isolated systolic hypertension, but the importance of cardiac contractility and ventricular ejection dynamics is also recognised.MethodsWe examined the contributions of arterial compliance and ventricular contractility to variations in aortic flow and increased central (cPP) and peripheral (pPP) pulse pressure, and PP amplification (PPa) in normotensive subjects during pharmacological modulation of physiology, in hypertensive subjects, and in silico using a cardiovascular model accounting for ventricular–aortic coupling. Reflections at the aortic root and from downstream vessels were quantified using emission and reflection coefficients, respectively.ResultscPP was strongly associated with contractility and compliance, whereas pPP and PPa were strongly associated with contractility. Increased contractility by inotropic stimulation increased peak aortic flow (323.9 ± 52.8 vs. 389.1 ± 65.1 ml/s), and the rate of increase (3193.6 ± 793.0 vs. 4848.3 ± 450.4 ml/s2) in aortic flow, leading to larger cPP (36.1 ± 8.8 vs. 59.0 ± 10.8 mmHg), pPP (56.9 ± 13.1 vs. 93.0 ± 17.0 mmHg) and PPa (20.8 ± 4.8 vs. 34.0 ± 7.3 mmHg). Increased compliance by vasodilation decreased cPP (62.2 ± 20.2 vs. 45.2 ± 17.8 mmHg) without altering dP/dt, pPP or PPa. The emission coefficient changed with increasing cPP, but the reflection coefficient did not. These results agreed with in silico data obtained by independently changing contractility/compliance over the range observed in vivo.ConclusionsVentricular contractility plays a key role in raising and amplifying PP, by altering aortic flow wave morphology.
Volatile emissions from past eruptions at La Soufrière de Guadeloupe (Lesser Antilles): insights into degassing processes and atmospheric impacts
Authors: Metcalfe Abigail , Moune Séverine , Moretti Roberto , Komorowski Jean-Christophe , Aubry Thomas J. 
Published: June 2023

Volatiles exert a critical control on volcanic eruption style and in turn impact the near source environment and global climate. La Soufrière de Guadeloupe in the Lesser Antilles has been experiencing volcanic unrest since 1992, increasing to a peak in 2018. The lack of data available on volatiles from past eruptions, and the well-developed hydrothermal system makes understanding deep-released volatile behaviour challenging. In this study, we analyse new melt inclusions and shed light on the volatile lifecycle and impacts at La Soufrière de Guadeloupe. We focus on four eruptions: 1657 CE (Vulcanian), 1010 CE (Plinian), 341 CE (Strombolian) and 5680 BCE (Plinian), and compare to the well-studied 1530 CE (Sub-Plinian) eruption. The maximum volatile content of these eruption melt inclusions are: 4.42 wt% H2O, 1700 CO2 ppm, 780 ppm S, 0.36 wt% Cl and 680 ppm F. We observe a decrease in S content over time indicating the whole system is evolving by early separation of FeS, resulting in a lower S content in younger magma. Using the CHOSETTO v1 model, we modelled degassing paths related to decompression at low pressures, suggesting the majority of S degassing has occurred during magma ascent. We also calculate the SO2 emissions using the petrologic method, and while the 1657 CE, 1530 CE and 341 CE eruptions have negligible emissions (0.0001–0.001 Mt of SO2), the 1010 CE and 5680 BCE eruptions (0.2 Mt and 0.3 Mt of SO2, respectively) are greater. Using the SO2 emissions and plume height, we calculated the climate forcing associated with each event. The 1010 CE and 5680 BCE Plinian eruptions produced a peak global mean stratospheric aerosol optical depth (SAOD) of 0.0055 and 0.0062, respectively. This suggests, that even the largest eruptions of La Soufrière de Guadeloupe did not exert a significant climate forcing individually, but are important contributors to the volcanic stratospheric sulfate aerosol background resulting from relatively moderate but frequent explosive eruptions. Overall, this study provides new insights into degassing processes and climate forcing not only at La Soufrière de Guadeloupe, but also for other basaltic-andesitic, magmatic-hydrothermal systems. These new constraints are vital particularly if the volcano is currently in a state of unrest and will contribute to improving monitoring crisis management and long-term planning.
Estimation of terrestrial heat flow in hydrothermally active areas based on long-term bedrock temperature observations: a case study of northwestern Yunnan, China
Authors: Wang Wenchao , Liu Qiongying , Chen Shunyun , Liu Peixun , Chen Lichun 
Published: June 2023

Terrestrial heat flow plays an important role in the study of plate tectonics, geothermal resource exploration and earthquake genesis. The measurement of terrestrial heat flow usually utilizes deep boreholes, which is expensive and inconvenient for high altitudes or mountainous terrain. In hydrothermally active areas, the temperature distribution is disturbed by heat convection, resulting in difficulty in obtaining conductive heat flow. In fact, heat can be used as a tracer to quantify groundwater flow. This article presents a method for calculating terrestrial heat flow suitable for hydrothermally active areas, which can correct the influence of groundwater flow to obtain the conductive heat flow reflecting the deep thermal background. The method uses temperature-time series at multiple depths of the shallow crust to calculate the groundwater flow rate. The convective heat flux component is then removed based on information on groundwater movement, and the conductive heat flow can be acquired. The feasibility of the method is verified by a theoretical model. This method has been applied to estimate terrestrial heat flows in northwestern Yunnan, China, which is a hydrothermally active area. The heat flow obtained through our method range from 54.5 to 130.3 mW/m2, with an average of 94.5 mW/m2, consistent with the high-quality measured heat flow values in the boreholes. This study provides new perspectives for acquiring terrestrial heat flow in areas that are affected by fluid activities.
Risk factors and survival prediction of young breast cancer patients with liver metastases: a population-based study
Authors: Pu Chen-Chen , Yin Lei , Yan Jian-Ming 
Published: June 2023

BackgroundThe risk and prognosis of young breast cancer (YBC) with liver metastases (YBCLM) remain unclear. Thus, this study aimed to determine the risk and prognostic factors in these patients and construct predictive nomogram models.MethodsThis population-based retrospective study was conducted using data of YBCLM patients from the Surveillance, Epidemiology, and End Results database between 2010 and 2019. Multivariate logistic and Cox regression analyses were used to identify independent risk and prognostic factors, which were used to construct the diagnostic and prognostic nomograms. The concordance index (C-index), calibration plot, receiver operating characteristic (ROC) curve, and decision curve analysis (DCA) were used to assess the performances of the established nomogram models. Propensity score matching (PSM) analysis was used to balance the baseline characteristics between the YBCLM patients and non-young patients with BCLM when comparing overall survival (OS) and cancer-specific survival (CSS).ResultsA total of 18,275 YBC were identified, of whom 400 had LM. T stage, N stage, molecular subtypes, and bone, lung, and brain metastases were independent risk factors for LM developing in YBC. The established diagnostic nomogram showed that bone metastases contributed the most risk of LM developing, with a C-index of 0.895 (95% confidence interval 0.877–0.913) for this nomogram model. YBCLM had better survival than non-young patients with BCLM in unmatched and matched cohorts after propensity score matching analysis. The multivariate Cox analysis demonstrated that molecular subtypes, surgery and bone, lung, and brain metastases were independently associated with OS and CSS, chemotherapy was an independent prognostic factor for OS, and marital status and T stage were independent prognostic factors for CSS. The C-indices for the OS- and CSS-specific nomograms were 0.728 (0.69–0.766) and 0.74 (0.696–0.778), respectively. The ROC analysis indicated that these models had excellent discriminatory power. The calibration curve also showed that the observed results were consistent with the predicted results. DCA showed that the developed nomogram models would be effective in clinical practice.ConclusionThe present study determined the risk and prognostic factors of YBCLM and further developed nomograms that can be used to effectively identify high-risk patients and predict survival outcomes.