bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of administered amount of peptide on tumor dosimetry at the first cycle of peptide receptor radionuclide therapy (PRRT) in relation to total tumor somatostatin receptor expression
Authors: Jahn Ulrika , Garske-Román Ulrike , Sandström Mattias , Lubberink Mark , Sundin Anders 
Published: May 2023

Abstract
Background
The accumulation of 177Lu-DOTATATE might be influenced by the amount of administered peptide in relation to the tumor somatostatin receptor expression. The effect of the administered peptide mass on the resulting absorbed dose in tumors and normal organs has not previously been assessed in relation to the patients’ tumor load.

Method
Patients with small intestinal (n = 141) and pancreatic (n = 62) neuroendocrine tumors (NETs) who underwent PRRT were selected for retrospective evaluation. All patients had received 7.4 GBq 177Lu-DOTATATE, and the amount of administered peptide in the preparation varied from 93 to 456 µg. The absorbed dose in tumors and normal tissue at the first PRRT cycle was calculated, based on SPECT-measurements at day 1, 4, and 7 post-infusion. The total tumor somatostatin receptor expression (tTSSTRE) was calculated on SPECT after 24 h by multiplying the functional tumor volume, delineated by 42% cut-off VOIs of the highest activity, with the SUVmean for the respective tumor VOIs. Spearman’s rank correlation analyzed any relationship between the administered amount of peptide and the absorbed dose in tumors and normal organs, in relation to the patients’ tTSSTRE.

Results
There was no correlation between the amount of peptide and any of the tested parameters in relation to tTSSTRE.

Conclusion
In this retrospective analysis, no correlation between the amount of administered peptide in the 177Lu-DOTATATE preparation and the absorbed radiation doses in tumors and normal tissues was demonstrated in relation to the total tumor SSTR expression.
Two decades of epidemiological surveillance of the pine wood nematode in France reveal its absence despite suitable conditions for its establishment
Authors: Mariette Nicolas , Hotte Hoël , Chappé Anne-Marie , Grosdidier Marie , Anthoine Géraldine , Sarniguet Corinne , Colnard Odile , Kersaudy Emmanuel , Paris Marie-Thérèse , Koen Emmanuel , Folcher Laurent 
Published: May 2023

Abstract
Key message
This study takes stock of the first 20 years (2000–2019) of monitoring the pine wood nematode (PWN) in metropolitan France. While PWN was never found in the wild during this period, it was reported in some wood-based commodities entering or circulating on French territory. This stresses the importance of remaining extremely vigilant, as the conditions found in France, especially weather conditions, could be particularly suitable for the pest’s establishment.

Context
The pine wood nematode (PWN) Bursaphelenchus xylophilus, responsible for pine wilt disease (PWD), is one of the most important forest tree pests worldwide. It is thus the focus of many monitoring programmes. In the European Union, for example, it is categorised as a priority quarantine pest, so each member state is obliged to monitor the PWN on its territory.

Aims
The first objective of this paper was to describe PWN monitoring in metropolitan France, namely how it is organised and whether it has led to the nematode’s detection. Secondly, we wished to investigate what the levels of PWD expression for host pines infected by B. xylophilus would be in France. Thirdly, we wanted to find out whether other Bursaphelenchus species had been found on French territory during these two decades of PWN monitoring.

Methods
We analysed data from samples collected in the framework of the monitoring programme between 2000 and 2019 to track the PWN in its host pines, its insect vector (Monochamus spp.) and in wood-based commodities imported into or circulating in metropolitan France. We also generated risk maps of PWD expression based on an evapo-transpiration model using climate data for the period 2000–2019.

Results
This monitoring, which was regularly reinforced from 2000 to 2019, consisted of sampling and analysing around 18,000 wood samples and 66,000 insects over this period. Although the PWN was not detected in pine stands or within its insect vector, some wood-based commodities were found to be contaminated. Risk maps of PWD expression show that in the most recent years (2015–2019), the weather conditions in a large fraction of metropolitan France were suited to PWD expression, mostly with a delay (i.e., latency) between infection and observable wilt symptoms. PWN monitoring has also revealed the presence of other Bursaphelenchus species, most of which were discovered for the first time in metropolitan France and are described herein.

Conclusion
While metropolitan France is still free of the PWN, this study emphasises the need to remain cautious as the French territory appears particularly suitable for this pest’s establishment. Furthermore, our research has led us to propose some ideas on how to improve PWN monitoring.
An improved dynamic programming tracking-before-detection algorithm based on LSTM network
Authors: Song Fei , Li Yong , Cheng Wei , Dong Limeng 
Published: May 2023

AbstractThe detection and tracking of small and weak maneuvering radar targets in complex electromagnetic environments is still a difficult problem to effectively solve. To address this problem, this paper proposes a dynamic programming tracking-before-detection method based on a long short-term memory (LSTM) network (LSTM-DP-TBD). With the predicted target motion state provided by the LSTM network, the state transition range of the traditional DP-TBD algorithm can be updated in real time, and the detection and tracking effect achieved for maneuvering small and weak targets is also improved. Utilizing the LSTM network to model the moving state of the target, the moving features of the maneuvering target can be learned from the noisy input data. By incorporating these features into the traditional DP-TBD algorithm, the state transition set can be adjusted in time with the changes in the moving state of the target so that the new algorithm is capable of effectively recursively accumulating the movement trend of the maneuvering small and weak target. Simulation results show that the new algorithm is able to effectively accomplish the task of detecting and tracking maneuvering small and weak targets, and it achieves improved detection and tracking probabilities.
Chromosome folding and prophage activation reveal specific genomic architecture for intestinal bacteria
Authors: Lamy-Besnier Quentin , Bignaud Amaury , Garneau Julian R. , Titecat Marie , Conti Devon E. , Von Strempel Alexandra , Monot Marc , Stecher Bärbel , Koszul Romain , Debarbieux Laurent , Marbouty Martial 
Published: May 2023

Abstract
Background
Bacteria and their viruses, bacteriophages, are the most abundant entities of the gut microbiota, a complex community of microorganisms associated with human health and disease. In this ecosystem, the interactions between these two key components are still largely unknown. In particular, the impact of the gut environment on bacteria and their associated prophages is yet to be deciphered.

Results
To gain insight into the activity of lysogenic bacteriophages within the context of their host genomes, we performed proximity ligation-based sequencing (Hi-C) in both in vitro and in vivo conditions on the 12 bacterial strains of the OMM12 synthetic bacterial community stably associated within mice gut (gnotobiotic mouse line OMM12). High-resolution contact maps of the chromosome 3D organization of the bacterial genomes revealed a wide diversity of architectures, differences between environments, and an overall stability over time in the gut of mice. The DNA contacts pointed at 3D signatures of prophages leading to 16 of them being predicted as functional. We also identified circularization signals and observed different 3D patterns between in vitro and in vivo conditions. Concurrent virome analysis showed that 11 of these prophages produced viral particles and that OMM12 mice do not carry other intestinal viruses.

Conclusions
The precise identification by Hi-C of functional and active prophages within bacterial communities will unlock the study of interactions between bacteriophages and bacteria across conditions (healthy vs disease).
Distribution of surface heat flow and effects on the subsurface temperatures in the northern part of Thrace Basin, NW Turkey
Authors: Erkan Kamil , Balkan-Pazvantoğlu Elif 
Published: May 2023

AbstractThe Thrace Basin in northwestern Turkey is a deep Eocene–Oligocene hydrocarbon-bearing sedimentary basin. The basin has potential for geothermal energy utilization in the future due to its favorable geological conditions. In this study, we combined the available bottom hole temperature (BHT) data from 70 points with the thermal conductivity and radiogenic heat productions of the basin formations, and generated a detailed thermal model of the northern part of the basin. For heat flow determinations from the BHT data, we applied Bullard’s thermal resistance method on formation thermal conductivities and thicknesses. The results give an average surface heat flow of 65.8 ± 11.3 mW/m2. We obtained high heat flow values (75–80 mW/m2) in the eastern and western sides, and the central part of the study area. These relatively high heat flow values can be explained by the combined effect of basement topography and the variations in the radiogenic heat production of the basement rocks. The calculated subsurface temperatures in selected hydrocarbon fields vary in the range of 45–64 °C at 1 km depth, 99–136 °C at 3 km depth, and 155–208 °C at 5 km depth as a result of local variations of the surface heat flow and formation thermal resistances. These variations in subsurface temperatures can have significant effects on the cost of geothermal energy production in future.
Non-indigenous preschool teachers’ culturally relevant pedagogy in the indigenous areas of Taiwan
Authors: Chen Bi-Ching 
Published: May 2023

AbstractThis study used the Ladson-Billings culturally relevant pedagogical approach to understanding non-indigenous preschool teachers’ multicultural literacy and practices in indigenous areas. Through purposive sampling, we selected six teachers with three years of teaching experience each in Eastern Taiwan as study participants. Interviews with them, classroom observation records, teaching files, students’ diaries, and researchers’ inspiring diaries were all used as data sources. The analyses were based on the Ladson-Billings culturally relevant pedagogical approach. There were three major results. First, non-indigenous teachers used indigenous language and culture and invited indigenous parents to cooperate with the tribe to increase their students’ academic achievement. Second, the teachers connected their indigenous students’ families’ backgrounds and experiences with their curriculums, maintained certain indigenous cultural norms in the classroom, and communicated with indigenous students’ parents in languages that were familiar to them. Third, the teachers devised strategies to overcome the gap between their teaching goals and beliefs about their students’ childcare centers and cultural backgrounds. The factor that most contributed to the success of non-indigenous preschool teachers teaching in indigenous was communicating with indigenous parents and encouraging them to join in school activities.
Pathological complete response with nivolumab for recurrence of liver metastasis after gastrectomy of gastric cancer
Authors: Jun Chen , Yamauchi Suguru , Yube Yukinori , Egawa Hiroki , Yoshimoto Yutaro , Kubota Akira , Tsuda Kenki , Kaji Sanae , Orita Hajime , Oka Shinichi , Mine Shinji , Fukunaga Tetsu 
Published: May 2023

Abstract
Background
Advanced gastric cancer has an unfavorable prognosis and poor curability. Immune checkpoint inhibitors, such as nivolumab, have recently emerged as a potential solution for this aggressive disease. However, there is a lack of established evidence on the clinical efficacy of these agents, particularly in the perioperative period for advanced gastric cancer patients who are unresectable, recurrent, or preoperative. Despite the limited data available, there have been rare cases of dramatic therapeutic effects. In this study, we present a successful case of nivolumab treatment along with surgery.

Case presentation
A 69-year-old female presented with pericardial discomfort and was diagnosed with advanced gastric cancer following upper gastrointestinal endoscopy. Laparoscopic distal gastrectomy with D2 lymph node dissection was performed, resulting in a final pathological diagnosis of Stage IIIA. The patient received postoperative adjuvant chemotherapy with oral S-1 therapy, but was found to have multiple liver metastases at 8 months postsurgery. Weekly paclitaxel and ramucirumab therapy was initiated, but the patient experienced adverse side effects, leading to the discontinuation of treatment. Nivolumab monotherapy was then administered for 18 cycles, resulting in a partial therapeutic response and PET–CT revealed a complete metabolic response. However, the patient developed a Grade 3 pemphigoid as an immune-related adverse event, leading to the cessation of nivolumab. The patient underwent laparoscopic partial hepatectomy. Postoperative pathology showed no residual tumor cells, indicating a complete response. At present, 25 months after surgery, the patient was alive without recurrence.

Conclusion
In this report, we present a case of gastric cancer with liver metastatic recurrence, in which a complete pathological response was achieved with nivolumab treatment. Although determining whether surgical intervention is necessary following successful drug treatment can be challenging, PET–CT imaging may be useful in decision-making regarding surgical treatment.
Exertional Heat Stroke and Rhabdomyolysis: A Medical Record Review and Patient Perspective on Management and Long-Term Symptoms
Authors: Kruijt Nick , van den Bersselaar L. R. , Hopman M. T. E. , Snoeck M. M. J. , van Rijswick M. , Wiggers T. G. H. , Jungbluth H. , Bongers C. C. W. G. , Voermans N. C. 
Published: May 2023

Abstract
Introduction
Exertional heat stroke (EHS) is a medical emergency, occurring when the body generates more heat than it can dissipate, and frequently associated with exertional rhabdomyolysis (ERM). In the present study we aimed to (I) identify clinical features and risk factors, (II) describe current prehospital management, (III) investigate long-term outcomes including the impact on mental health, and review the guidance received during restarting activities. We hope that our approach will improve individual and organizational heat illness preparedness, and improve follow-up care.

Methods
We performed a prospective online survey and retrospective medical record review among athletes and military personnel with an episode of EHS/ERM in the Netherlands between 2010 and 2020. We evaluated prehospital management, risk factors, clinical features and long-term outcomes at 6 and 12 months after the event, including mental health symptoms. Furthermore, we investigated what guidance participants received during follow-up, and assessed the patients’ perspective on these outcomes.

Results
Sixty participants were included, 42 male (70%) and 18 female (30%), of which 47 presented with EHS (78%) and 13 with ERM (22%). Prehospital management was inconsistent and in the majority of participants not conducted according to available guidelines. Self-reported risk factors included not feeling well-acclimatized to environmental heat (55%) and peer pressure (28%). Self-reported long-term symptoms included muscle symptoms at rest (26%) or during exercise (28%), and neurological sequelae (11%). Validated questionnaires (CIS, HADS and SF-36) were indicative of severe fatigue (30%) or mood/anxiety disorders (11%). Moreover, 90% expressed a lack of follow-up care and that a more frequent and intensive follow-up would have been beneficial for their recovery process.

Conclusion
Our findings indicate major inconsistencies in the management of patients with EHS/ERM, emphasizing the compelling need for implementing standardized protocols. Based on the results of long-term outcome measures, we recommend to counsel and evaluate every patient not only immediately after the event, but also in the long-term.
Occurrence of plant pathogenic Pseudomonas syringae in the Danube River Basin: abundance and diversity assessment
Authors: Anteljević Marina , Rosić Iva , Medić Olja , Kolarević Stoimir , Berić Tanja , Stanković Slaviša , Nikolić Ivan 
Published: May 2023

AbstractPlant pathogenic strains of Pseudomonas syringae (Psy) spp. have been detected in nonagricultural habitats, including those associated with the water cycle. Their presence in aquatic systems allows dissemination over long distances, especially with irrigation practices. In May 2021, we sampled 15 sites along the Danube River Basin in Serbia to gain insight into P. syringae abundance and diversity. We identified 79 Psy strains using Psy-specific primers, and a partial sequence of the citrate synthase (cts) house-keeping gene has served for phylogenetic diversity assessments. Phenotypic diversity determination included characterizing features linked with survival and pathogenic lifestyle. The ice nucleation activity, pectinolytic activity, swimming and swarming assays, and hypersensitive reaction on plants were tested. Psy was detected at ten of 15 sites examined at abundance ranging from 1.0 × 102 to 1.2 × 104 CFU/L. We discovered the presence of four phylogroups, with phylogroup 2 being the most abundant, followed by phylogroups 7, 9, and 13. The hypersensitive reaction was induced by 68.63% of the isolates from the collection. A partial sequence comparison of the cts gene showed 100% similarity between isolates from cherry plants epidemics in Serbia caused by Psy and isolates from the Danube River. Our results suggest that the Danube River, extensively used for irrigation of agricultural fields, harbors diverse strains of Psy, which possess various features that could lead to potential disease outbreaks on crops. This study represents the first in-depth analysis of Psy abundance and diversity in the Danube River Basin. It sets the ground for future pre-epidemic studies and seasonal monitoring of Psy population dynamics.
Diagnostic accuracy of subjective kinetic assessment of masses in contrast-enhanced mammography in comparison with contrast-enhanced magnetic resonance imaging
Authors: Subramaniam Prema , Renganathan Rupa , Suganya P. , Mandal Adrija 
Published: May 2023

AbstractBackgroundContrast-enhanced MRI (CE MRI) of the breast is currently the most sensitive imaging technique for detecting invasive breast cancer, and it provides both morphologic and functional information through kinetics for characterizing breast masses. Contrast-enhanced mammography (CEM) uses the same principle of neo-angiogenesis to detect early cancers similar to MRI with comparable diagnostic performance. However, there is an important limitation in CEM in characterizing the breast lesions because of the non-availability of kinetic information. To the best of our knowledge, very few studies have assessed the CEM kinetics. In this study, we have evaluated the accuracy of subjective assessment of contrast kinetics in CEM and compared it with the subjective and quantitative kinetic assessment in CE MRI. If the performance of CEM is comparable to MRI, it may add an additional dimension to CEM in characterizing the breast masses in addition to detection.ResultsKinetic information of 123 lesions in 90 patients was analyzed in CEM and MRI. Of these, 26 (21.1%) were benign, 4 (3.3%) were high risk lesions, and 93 (75.6%) were malignant breast lesions. Comparison of subjective and quantitative assessment in CE MRI had almost perfect agreement with a kappa value of 0.816, and both were used as reference standards for comparing CEM kinetics. Comparison of subjective assessment of kinetic patterns in CEM using only CC and MLO views showed moderate agreement with both quantitative (kappa − 0.483) and subjective (0.547) CE MRI kinetics. When the delayed image obtained at 8 min was included for kinetic analysis, CEM kinetics showed substantial to almost perfect agreement with quantitative (kappa − 0.673) and subjective (kappa − 0.855) CE MRI kinetics, respectively.ConclusionWe hope that this study results would encourage the breast radiologist to assess the kinetic information from CEM and use CEM as a single, simple and cost-effective imaging modality in detecting and characterizing breast masses.
Effect of 25-hydroxyvitamin D levels on the internalising dimension as a transdiagnostic risk factor: Mendelian randomisation study
Authors: Konzok Julian , Baumeister Sebastian-Edgar , Winkler Thomas W. , Leitzmann Michael F. , Baurecht Hansjörg 
Published: May 2023

BackgroundObservational studies indicate a relationship between vitamin D (25-hydroxyvitamin D; 25OHD) deficiency and the development of internalising disorders, especially depression. However, causal inference approaches (e.g. Mendelian randomisation) did not confirm this relationship. Findings from biobehavioural research suggests that new insights are revealed when focusing on psychopathological dimensions rather than on clinical diagnoses. This study provides further evidence on the relationship between 25OHD and the internalising dimension.AimsThis investigation aimed at examining the causality between 25OHD and internalising disorders including a common internalising factor.MethodWe performed a two-sample Mendelian randomisation using genome-wide association study (GWAS) summary data for 25OHD (417 580 participants), major depressive disorder (45 591 cases; 97 674 controls), anxiety (5580 cases; 11 730 controls), post-traumatic stress disorder (12 080 cases; 33 446 controls), panic disorder (2248 cases; 7992 controls), obsessive–compulsive disorder (2688 cases; 7037 controls) and anorexia nervosa (16 992 cases; 55 525 controls). GWAS results of the internalising phenotypes were combined to a common factor representing the internalising dimension. We performed several complementary analyses to reduce the risk of pleiotropy and used a second 25OHD GWAS for replication.ResultsWe found no causal relationship between 25OHD and any of the internalising phenotypes studied, nor with the common internalising factor. Several pleiotropy-robust methods corroborated the null association.ConclusionsFollowing current transdiagnostic approaches to investigate mental disorders, our results focused on the shared genetic basis between different internalising phenotypes and provide no evidence for an effect of 25OHD on the internalising dimension.
α-Ketoglutarate-dependent Enzymes in Breast Cancer and Therapeutic Implications
Authors: Xiong Jingjing , Yan Chaojun , Zhang Qing , Zhang Jing 
Published: May 2023

Abstract
α-Ketoglutarate (αKG)-dependent dioxygenases are a superfamily of enzymes that require oxygen, reduced iron, and αKG for their catalytic functions. Therefore, they have the potential to sense the availabilities of oxygen, iron, and specific metabolites, including αKG and its structurally related metabolites. These enzymes play essential roles in various biological processes, including cellular adaptation to hypoxia, epigenetic and epitranscriptomic regulation of gene expression, and metabolic reprogramming. Many αKG-dependent dioxygenases are dysregulated in cancer pathogenesis. Herein, we review how they are regulated and function in breast cancer, which may offer new therapeutic intervention strategies for targeting this family of enzymes.