bluuebunny/crossref_metadata_2025_split huggingface dataset:
Classical DC2 subsets and monocyte‐derived DC: Delineating the developmental and functional relationship
Authors: Backer Ronald A. , Probst Hans Christian , Clausen Björn E. 
Published: January 2023

AbstractTo specifically tailor immune responses to a given pathogenic threat, dendritic cells (DC) are highly heterogeneous and comprise many specialized subtypes, including conventional DC (cDC) and monocyte‐derived DC (MoDC), each with distinct developmental and functional characteristics. However, the functional relationship between cDC and MoDC is not fully understood, as the overlapping phenotypes of certain type 2 cDC (cDC2) subsets and MoDC do not allow satisfactory distinction of these cells in the tissue, particularly during inflammation. However, precise cDC2 and MoDC classification is required for studies addressing how these diverse cell types control immune responses and is therefore currently one of the major interests in the field of cDC research. This review will revise murine cDC2 and MoDC biology in the steady state and under inflammatory conditions and discusses the commonalities and differences between ESAMlo cDC2, inflammatory cDC2, and MoDC and their relative contribution to the initiation, propagation, and regulation of immune responses.
Asthma and allergic diseases: Cross talk of immune system and environmental factors
Authors: Beerweiler Claudia C. , Masanetz Rebecca K. , Schaub Bianca 
Published: January 2023

AbstractAir pollution and immune‐related diseases including allergy and asthma are constantly on the rise worldwide. Thus, a comprehensive investigation of environmentally induced immune regulation is required for a deeper understanding of disease pathogenesis, progression as well as prevention. Here, we summarize the current knowledge on environmental factors such as microbiome or geographical locations with harmful or protective effects for human health and their different routes of exposure. This review comprises a brief outline regarding the latest findings on the interaction of environmental factors with innate and adaptive regulation of the immune system, exemplarily for one protective and one harmful environmental factor, respectively.
Fabrication of Defect‐Engineered MOF‐801 Membrane for Efficient Dye Rejection
Authors: Yan Fuyuan , Yan Jiahui , Sun Yanwei , Ji Taotao , He Yongfu , Guo Xinwen , Liu Yi 
Published: January 2023

AbstractAlthough having shown great promise for efficient water treatment, rational structural design and engineering of polycrystalline MOF membranes remain rarely investigated so far. In this study, we prepared well‐intergrown MOF‐801 membranes with tailorable structural deficiencies in the framework for application in dye rejection. Of particular note, we found that the addition of formic acid as modulator led to the formation of MOF‐801 membrane with higher missing‐linker number, which was beneficial for increasing water flux with little compromise in dye rejection rate. The MOF‐801 membrane prepared in this work exhibited excellent dye rejection performance (CR rejection rate of 99.50 % and water flux of 31.69 L m−2 h−1 bar−1) as well as excellent long‐term stability.
Research on the prediction and relationship between academic attention and network attention in chemistry teaching
Authors: Song Rui , Li Mingjiang , Zhao Yulin , Liu Kai , Li Junke , Zhou Jincheng 
Published: January 2023

AbstractIn order to adapt to the development of modern education and provide education practitioners with decision‐making suggestions, it is necessary to understand the relationship between the public's attention to basic chemistry education (BCE) and academic attention. However, many existing research is based on a single platform to study social hot information, such as Google Index, Baidu Index, Web of Science, and so on. But they ignore the relationship that exists between the Baidu Index and related academic platforms, and ignore the common information reflected between them. This paper takes advantage of the big data method, through the big data of Baidu Index and the big data obtained from China National Knowledge Internet (CNKI) database, to study the network attention and academic attention of BCE, and propose a CVS‐LSP‐GP framework. It first selects keywords through correlation analysis, secondly uses the data obtained from the first step to construct a nonlinear regression model, and finally combines the results of gray prediction to predict the academic attention of CNKI related to BCE. The research results show that the BCE is mainly affected by the micro‐lecture teaching mode, and relevant education practitioners should integrate the micro‐lecture mode into teaching for more further research and practice.
Theoretical, numerical, and experimental investigation on the compliance and natural frequency of sinusoidal flexure hinges
Authors: Wang Qiliang , Long Yiping , Wei Jianming , Hu Junfeng , Yang Youwen 
Published: January 2023

AbstractTo design a flexure hinge with high precision and high natural frequency, the sinusoidal flexure hinge is proposed in this article. First, the formulae for the compliance and precision factors of the hinge were derived based on the Euler–Bernoulli beam theory and the Gauss–Legendre quadrature formula. The natural frequency was also investigated based on the transfer matrix method. Compared with the simulation results of ANSYS Workbench, the results show that the modeling error is less than 6.7%. Second, the influence of structural parameters on compliance, precision factor, compliance precision ratio, and natural frequency was analyzed. The results show that compliance and precision are often contradictory, and the minimum thickness significantly influences the hinge's performance. Compared with conic flexure hinges in terms of compliance, precision, compliance precision ratios, and natural frequency, the sinusoidal flexure hinges have a better comprehensive performance. Finally, a flexure hinge was manufactured, and compliance was measured. The experimental results show that the error between the experimental value and the modeling value is 7.8%. Both simulation and experimental results verify the effectiveness of the sinusoidal flexure hinge model.
Performance recovery of a repaired 4‐storey reinforced concrete structure subjected to shake‐table testing
Authors: Shegay Alex V. , Miura Kota , Akira Mikawa , Maeda Masaki , Seki Matsutaro 
Published: January 2023

AbstractUnderstanding the effects of repair on reinforced concrete (RC) structures is critical to evaluating their performance in future earthquakes. In this research, a system‐level approach is taken by repairing a previously damaged ¼ scale 4‐storey RC structure and subjecting it to a series of dynamic excitations via shake table testing. The repair was undertaken using common construction materials with the primary motivation of restoring original performance. The performance of the repaired structure was compared to that of the original structure prior to repairs. It was found that overall initial stiffness of the structure was recovered to 66% of the original initial stiffness and was generally higher in floors where more intensive repair works were undertaken. Damping capacity was quantified via equivalent viscous damping ratio using three different methods. Regardless of the calculation method, the damping capacity recovery was around 80% for low‐moderate drifts and 100% for high drifts. While collapse was not induced in the structure, drift ratio demands of over 5% could be achieved both before and after repairs, suggesting a full practical recovery of deformation capacity. Structural strength of the repaired structure was found to be 17% higher than that of the original structure.
The role of expectations for currency crisis dynamics—The case of the Turkish lira
Authors: Beckmann Joscha , Czudaj Robert L. 
Published: January 2023

AbstractThis paper examines whether and how expectations have contributed to the turbulent path of the Turkish lira since 2008. We derive uncertainty measures surrounding gross domestic product (GDP) growth, inflation, the interest rate, and exchange rates based on survey data from Consensus Economics. Our results illustrate that forecasts have affected realized exchange rates and stock market returns via increased uncertainty. We also show that expectations regarding monetary policy have changed throughout the sample period. In line with, a gradual adjustment of expectations professionals have accounted for the violation of the Taylor rule.
Coastal changes and human occupation in the eastern part of Thessaloniki Bay: Geoarchaeological investigations of Toumba Gona
Authors: Niebieszczański Jakub , Hildebrandt‐Radke Iwona , Czebreszuk Janusz , Bahyrycz Cezary , Vouvalidis Konstantinos , Syrides Georgios , Tsourlos Panagiotis , Pappa Maria , Andreou Stylianos 
Published: January 2023

AbstractThe Holocene marine transgression in the Aegean Sea area has significantly impacted prehistoric societies. Toumba Gona is a tell site located at the mouth of the Anthemous River, east of Thessaloniki Bay. According to earlier research, the site should be dated at most to the late stage of the Early Bronze Age. Geoarchaeological research by means of electrical resistivity tomography, vibra‐coring, sedimentological analysis, and radiocarbon dating shows, however, that the direct proximity of the site witnessed the maximum marine transgression around 3000–2500 BC and the human habitation phase before the 3rd millennium BC, before the transgression. The coastline began to recede due to the delta progradation, which resulted in the formation of extensive marshes to the south of the Toumba. The increased fluvial activity since the end of the 4th millennium BC is relatable with the progradation of the Anthemous River's bay head delta. Human occupation was recorded before and after the marine transgression thus suggesting human persistence in the coastline environment.
Astrocytic Kir4.1 channels regulate locomotion by orchestrating neuronal rhythmicity in the spinal network
Authors: Barbay Tony , Pecchi Emilie , Ducrocq Myriam , Rouach Nathalie , Brocard Frédéric , Bos Rémi 
Published: January 2023

AbstractNeuronal rhythmogenesis in the spinal cord is correlated with variations in extracellular K+ levels ([K+]e). Astrocytes play important role in [K+]e homeostasis and compute neuronal information. Yet it is unclear how neuronal oscillations are regulated by astrocytic K+ homeostasis. Here we identify the astrocytic inward‐rectifying K+ channel Kir4.1 (a.k.a. Kcnj10) as a key molecular player for neuronal rhythmicity in the spinal central pattern generator (CPG). By combining two‐photon calcium imaging with electrophysiology, immunohistochemistry and genetic tools, we report that astrocytes display Ca2+ transients before and during oscillations of neighboring neurons. Inhibition of astrocytic Ca2+ transients with BAPTA decreases the barium‐sensitive Kir4.1 current responsible of K+ clearance. Finally, we show in mice that Kir4.1 knockdown in astrocytes progressively prevents neuronal oscillations and alters the locomotor pattern resulting in lower motor performances in challenging tasks. These data identify astroglial Kir4.1 channels as key regulators of neuronal rhythmogenesis in the CPG driving locomotion.
The regulatory roles of socio‐economic status, social and intellectual activity in the relationship between alcohol consumption and cognitive decline trajectory in middle‐aged and elderly Chinese: A prospective cohort study
Authors: Cheng Fang , Ju Yanli , Chen Xueyu , Jia Hongying 
Published: January 2023

AbstractObjectivesTo determine the relationship between alcohol consumption and cognitive decline, and to further explore the potential regulatory role of education, socio‐economic status (SES), and social or intellectual activity in this relationship.Methods6197 participants aged 45–75 years with four repeated measures data from 2011 to 2018 were included. A mixed‐effect model was used to explore the relationship between alcohol consumption and the rate of change in cognitive decline, a latent class growth mixed model (LCGMM) was applied to determine the potential trajectory of cognitive decline, and finally, the mediating and moderating analyses were used to determine the regulatory effect of all four variables on the relationship between alcohol consumption and potential trajectory.ResultsCompared to never‐drinkers, moderate alcohol consumption was a protective factor for overall cognitive function (β = 0.13, 95% CI: 0.04–0.20, p < 0.001), but there was no statistical correlation with the decline rate of cognitive function. And this protective effect was no longer significant after additional adjustments for education, SES, social and intellectual activity. The LCGMM model divided participants into two trajectories, a high‐level‐to‐decline group including 79.75% of participants (quadratic: β [SE]: −0.90 [0.07], p < 0.001), and a low‐level‐to‐decline group including 20.25% participants (linear: β [SE]: −3.05 [0.49], p < 0.001). With the latter as the reference, SES played a reverse regulation role in the harmful effect of heavy drinking on cognitive trajectories (odd ratio [OR] = 0.46, 95% CI: 0.23–0.93, p < 0.05). Social and intellectual activities played a negative mediating role in the harmful effect of alcohol consumption on cognitive trajectories (light: OR = 0.96, p < 0.001; moderate: OR = 0.96, p < 0.001; heavy: OR = 0.97, p < 0.01).ConclusionsAlcohol itself has no protective effect on the decline of longitudinal cognitive trajectory. But the regulatory effect of SES, social and intellectual activities slows down the harm of alcohol consumption on the decline of cognitive function.Clinical Trial RegistrationThe data used in this study are from publicly available databases. They are retrospective cohort studies without any intervention. Therefore, no clinical trial registration has been conducted.
Genomic profiling and precision medicine in complex ameloblastoma
Authors: Gates James C. , Clark Allison P. , Cherkas Elliot , Shreenivas Aditya V. , Kraus Dennis , Danzinger Natalie , Huang Richard S. P. , Johnson Jennifer , Ross Jeffrey S. 
Published: January 2023

AbstractBackgroundAmeloblastoma may present a significant treatment challenge in the locally advanced, recurrent and metastatic setting. Comprehensive genomic profiling (CGP) can identify targetable genomic alterations to aid in treatment.MethodsAmeloblastoma samples were sequenced using hybrid‐capture based sequencing. A systematic literature review was performed to examine outcomes in studies employing targeted treatment in ameloblastoma.ResultsWe reviewed 14 cases of Ameloblastoma using CGP. There were six patients with activating BRAF mutations, five with PIK3CA, five with SMO, four with FGFR2, one with EGFR, and one with ROS1. All cases were MSI stable and the median TMB was 2.5 mutations/Mb. A separate literature review of clinical outcomes in ameloblastoma showed a predominance of at least partial response to targeted treatment (7/12 cases).ConclusionCGP is helpful in identifying specific driver mutations in patients with complex ameloblastoma. Targeted treatment has been employed with success in achieving treatment response.
Characterization and phylogenetic analysis of Iranian SARS‐CoV‐2 genomes: A phylogenomic study
Authors: Aliabadi Nasrin , Jamaliduost Marzieh , Pouladfar Gholamreza , Marandi Nahid H. , Ziyaeyan Mazyar 
Published: January 2023

AbstractBackground and AimCharacterization of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) based on analyzing the evolution and mutations of viruses is crucial for tracking viral infections, potential mutants, and other pathogens. The purpose was to study the complete sequences of SARS‐CoV‐2 to reveal genetic distance and mutation rate among different provinces of Iran.MethodsAs of March 2020–April 2021, a total of 131 SARS‐CoV‐2 whole genome sequences submitted from Tehran and 133 SARS‐CoV‐2 full‐length sequences from 24 cities with high coverage submitted to EpiCoV GISAID database were analyzed to infer clades and mutation annotation compared with the wild‐type variant Wuhan‐Hu‐1.ResultsThe results of variant annotation were revealed 11,204 and 9468 distinct genomes were identified among the samples from different cities and Tehran, respectively. The phylogenetic analysis of genomic sequences showed the presence of eight GISAID clades, namely GH, GR, O, GRY, G, GK, L, and GV, and six Nextstrain clades; that is, 19A, 20A, 20B, 20I (alpha, V1), 20H (Beta, V2), and 21I (Delta) in Iran. The GH (GISAID clade), 20A (Nextstrain clade), and B.1 (Pango lineage) were predominant in Iran. Notably, analysis of the spike protein revealed D614G mutation (S_D614G) in 56% of the sequences. Also, the delta variant of the coronavirus, the super‐infectious strain that was first identified among the sequences submitted from the southern cities of the country such as Zahedan, Yazd and Bushehr, and most likely from these places to other cities of Iran as well has expanded.ConclusionsOur results indicate that most of the circulated viruses in Iran in the early time of the pandemic had collected in eight GISAID clades. Therefore, a continuous and extensive genome sequence analysis would be necessary to understand the genomic epidemiology of SARS‐CoV‐2 in Iran.
lncRNA NEAT1/miR‐495‐3p regulates angiogenesis in burn sepsis through the TGF‐β1 and SMAD signaling pathways
Authors: Meng Yanbin , Hao Zhenming , Zhang Hairui , Bai Peiyi , Guo Wanli , Tian Xiaorui , Xu Jun 
Published: January 2023

AbstractIntroductionTo investigate the role of the long‐chain noncoding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) in the process of angiogenesis in human umbilical vein endothelial cells (HUVECs) and illustrate its potential role in burn sepsis (BS) pathogenesis.MethodsHUVECs were treated with BS patient serum or healthy control serum. NEAT1 shRNA, miR‐495‐3p mimics, and miR‐495‐3p inhibitor were transfected into HUVECs. NEAT1 and miR‐495‐3 levels in serum or HUVECs were detected using quantitative reverse transcription‐polymerase chain reaction. Cell counting kit‐8 and flow cytometry assays were used to explore the proliferation and apoptosis of HUVECs. The expression of vascular endothelial growth factor (VEGF) in the supernatant was detected using enzyme‐linked immunosorbent assay. Tube formation of HUVECs was also analyzed. Western blot analysis was used to analyze signaling pathway proteins.ResultsIn HUVECs stimulated with BS patient serum, NEAT1 expression was increased, while miR‐495‐3p expression was decreased. In addition, NEAT1 silencing by specific shRNA inhibited cell proliferation, VEGF production, and tube formation under burn patient serum treatment, which decreased the TGFβ1/SMAD signaling pathway activation. Moreover, miR‐495‐3p minics inhibited angiogenesis and the activation of signaling pathways induced by NEAT1 shRNA. Furthermore, miR‐495‐3p inhobitor promoted angiogenesis in HUVECs and activated the TGFβ1/SMAD signaling pathway. In patients with BS, NEAT1 expression was significantly increased and miR‐495‐3p expression was decreased compared to healthy controls, and NEAT1 and miR‐495‐3p expression was associated with the clinical features of patients.ConclusionsOur results indicate that lncRNA NEAT1 regulates angiogenesis and activates the TGFβ1/SMAD signaling pathway during the occurrence of BS.
Resisting subtractive language and literacy policies: Breaking the cycles of loss among bilingual preservice educators
Authors: Harvey‐Torres Rosalyn , Cervantes‐Soon Claudia , Brochin Carol 
Published: January 2023

AbstractLiteracy scholars have noted the importance of drawing upon all of one's linguistic resources and experiences to make sense of texts. However, literacy instruction is often shaped by restrictive and punitive policies that limit the learning experiences and opportunities offered to racialized bi/multilingual students from low‐income communities. We illustrate this phenomenon through our experiences as former teachers of bilingual students and as teacher educators in Arizona and Texas, shedding light on the cycles of loss that such policies create and how this loss manifests among preservice teachers. We also reflect on the agency enacted by educators to heal their identities and develop transformative literacy practices.
A computational two‐photon fluorescence approach for revealing label‐free the 3D image of viruses and bacteria
Authors: Delmon Cédric , Ferrandon Erwan , Chouzenoux Emilie , Prorot Audrey , Alain Sophie , Lefort Claire 
Published: January 2023

AbstractCurrent solutions for bacteria and viruses identification are based on time‐consuming technics with complex preparation procedures. In the present work, we revealed label‐free the presence of free viral particles and bacteria with a computational two‐photon fluorescence (C‐TPF) strategy. Six bacteria were tested: Escherichia coli, Staphylococcus epidermidis, Proteus vulgaris, Pseudomonas fluorescens, Bacillus subtilis, and Clostridium perfringens. The two families of viral particles were the herpes virus with the cytomegalovirus (CMV, 300 nm of diameter) and the coronavirus with the SARS‐CoV‐2 (100 nm of diameter). The instrumental and computational pipeline FAMOUS optimized the produced 3D images. The origin of the fluorescence emission was discussed for bacteria regarding to their two‐photon excitation spectra and attributed to the metabolic indicators (FAD and NADH). The optical and computational strategy constitute a new approach for imaging label‐free viral particles and bacteria and paves the way to a new understanding of viral or bacterial ways of infection.
Overexpression of YTHDC2 contributes to the progression of prostate cancer and predicts poor outcomes in patients with prostate cancer
Authors: Song Jukun , You Ganhua , Yin Xinhai , Zhu Guohua , Wang Wei , Yu Yongwei , Zhu Jianguo 
Published: January 2023

AbstractYTH domain‐containing protein 2 (YTHDC2), a member of N6‐methyladenosine (m6A) readers, has been reported to be closely associated with multiple cancer types. However, very little is known about the YTHDC2 gene and its involvement in prostate cancer. YTHDC2 protein expression level was analyzed and correlated to clinical outcomes in prostate cancer patients who underwent prostatectomy in Guizhou Provincial People's Hospital. The YTHDC2 expression level was also detected in prostate cancer cell lines and an immortalized prostate epithelial cell line BPH‐1 and RWPE1 by quantitative real‐time reverse transcription polymerase chain reaction. Furthermore, we established stable cell lines (DU145 and PC‐3) transfected with either empty vector or the full‐length YTHDC2 gene and conducted cell function assays in vitro. Fisher's exact test and Pearson χ2 test were employed, Kaplan–Meier method was used for the survival analysis. Of 32 patient samples who enrolled in this study, YTHDC2 was significantly upregulated in prostate cancer (PCa) patients with higher Gleason scores and serum prostate‐specific antigen levels. YTHDC2 expression was significantly elevated in all PCa cell lines compared to BPH‐1 and RWPE1 (all p < 0.05). Functionally, the enforced expression of YTHDC2 markedly promoted cell growth, migration, and invasion efficacies in prostate cancer cells. Our data indicate that YTHDC2 upregulation may be potentially associated with the prognosis of prostate cancer patients.