bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome-wide analysis of primary human endothelial cell responses to 1 hour of protein translation inhibition identify nonsense mediated decay targets and a non-codingSLC11A2exon as an acute biomarker
Authors: Bielowka Adrianna M. , Govani Fatima S. , Patel Dilip , Bernabeu-Herrero Maria E , Li Dongyang , Aldred Micheala A. , Mollet Inês G. , Shovlin Claire L 
Published: September 2023

ABSTRACTNonsense mediated decay (NMD) lowers the cellular concentration of spliced RNAs harboring premature termination codons (PTC), and inhibition has been proposed as a potential therapeutic method. Conversely, NMD plays regulatory roles throughout the eukaryotic kingdom, including when protein translation is inhibited acutely as part of the integrated stress response. To define tools for endothelial evaluations of therapeutic NMD inhibition, and quantification of subtle cellular stress states, natural endothelial-expressed targets were examined via whole transcriptome RNA sequencing of primary human microvascular endothelial cells (HMECs) treated for 1h with cycloheximide, a protein translation and NMD inhibitor. Genes differentially expressed after 1h cycloheximide overlapped with genes differentially expressed many days after NMD-specific knockdown in other cell types. For endothelial cells, customized novel scripts used 255,500 exons in media-treated HMEC and 261,725 exons in cycloheximide-treated HMEC to predict 1h cycloheximide-stabilized exons. RT-PCR and RNASeq validations in other endothelial cells highlighted exon 3B of the iron transporterSLC11A2(also known asNRAMP2/DMT1) as a novel exon in a transcript most consistently stabilized. Exact junctional alignments toSLC11A2exon 3B were confirmed in blood outgrowth endothelial cells (BOECs) from 3 donors at mean 5.9% (standard deviation 2.0%) of adjacent constitutive exon expression, increasing 3.7-fold following 1h treatment with cycloheximide. Relevance beyond endothelial cells is supported bySLC11A2’swide expression profiles, genome-wide associations with microcytic anemia, biomarker status for poor prognosis ovarian cancer, and exon 3B sequence in RefSeq non-coding transcript NR_183176.1. The studies contribute understanding to functions affected acutely by NMD/translation inhibition and provide a stimulus for further studies in experimental, stress, and therapeutic settings.
A genetic circuit on a single DNA molecule as autonomous dissipative nanodevice
Authors: Greiss Ferdinand , Lardon Nicolas , Schütz Leonie , Barak Yoav , Daube Shirley S. , Weinhold Elmar , Noireaux Vincent , Bar-Ziv Roy 
Published: September 2023

AbstractRealizing genetic circuits on single DNA molecules as self-encoded dissipative nanodevices is a major step toward miniaturization of autonomous biological systems. A circuit operating on a single DNA implies that genetically encoded proteins localize during coupled transcriptiontranslation to DNA, but a single-molecule measurement demonstrating this has remained a challenge. Here, we used a genetically encoded fluorescent reporter system with improved spatiotemporal resolution and observed the synthesis of individual proteins tethered to a DNA molecule by transient complexes of RNA polymerase, messenger RNA, and ribosome. Against expectations in dilute cell-free conditions where equilibrium considerations favor dispersion, these nascent proteins linger long enough to regulate cascaded reactions on the same DNA. We rationally designed a pulsatile genetic circuit by encoding an activator and repressor in feedback on the same DNA molecule. Driven by the local synthesis of only several proteins per hour and gene, the circuit dynamics exhibited enhanced variability between individual DNA molecules, and fluctuations with a broad power spectrum. Our results demonstrate that coexpressional localization, as a nonequilibrium process, facilitates single-DNA genetic circuits as dissipative nanodevices, with implications for nanobiotechnology applications and artificial cell design.
Cell Catcher: a new method to extract and preserve live renal cells from urine
Authors: Nazmutdinova Katia , Man Cheuk Yan , Carter Martyn , Beales Philip L , Winyard Paul JD , Walsh Stephen B , Price Karen L , Long David A 
Published: September 2023

AbstractPatient-specific urine-derived cells are valuable tools for biomedical research and personalised medicine since collection is non-invasive and easily repeated, unlike biopsies. The full potential of urine-derived cells remains untapped, however, due to the short shelf life of samples and necessity for prompt centrifugation. This study aims to address this limitation by evaluating a novel filtration-based Cell Catcher device and comparing its efficiency to centrifugation. We obtained urine from 18 tubulopathy patients and using paired analysis demonstrated that the Cell Catcher device significantly improves the success rate of isolating viable renal cells, and the cell yield. The findings were confirmed in a second independent study, using 44 samples obtained from healthy controls or patients with Bardet-Biedl syndrome or tubulopathies, where colonies were established in 90% of the Cell Catcher-processed samples. Cultured cells displayed a variety of morphologies and expressed markers of podocyte and proximal tubule cells. Collectively, we describe an improved, point-of-care methodology to obtain live patient cells from urine using a filtration technique, with potential personalised medicine applications in nephrology, regenerative medicine, and urological cancers.
Bioluminescent detection of viral surface proteins using branched multivalent protein switches
Authors: Gräwe Alexander , Spruit Cindy M. , de Vries Robert P. , Merkx Maarten 
Published: September 2023

AbstractFast and reliable virus diagnostics is key to prevent the spread of viruses in populations. A hallmark of viruses is the presence of multivalent surface proteins, a property that can be harnessed to control conformational switching in sensor proteins. Here, we introduce a new sensor platform (dark-LUX) for the detection of viral surface proteins consisting of a general bioluminescent framework that can be post-translationally functionalized with separately expressed binding domains. The platform relies on 1) plug-and-play bioconjugation of different binding proteins via SpyTag/SpyCatcher technology to create branched protein structures, 2) an optimized turn-on bioluminescent switch based on complementation of the split-luciferase NanoBiT upon target binding and 3) straightforward exploration of the protein linker space. The influenza A virus (IAV) surface proteins hemagglutinin (HA) and neuraminidase (NA) were used as relevant multivalent targets to establish proof of principle and optimize relevant parameters such as linker properties, choice of target binding domains and the optimal combination of the competing NanoBiT components SmBiT and DarkBiT. The sensor framework allows rapid conjugation and exchange of various binding domains including scFvs, nanobodies andde novodesigned binders for a variety of targets, including the construction of a heterobivalent switch that targets the head and stem region of hemagglutinin. The modularity of the platform thus allows straightforward optimization of binding domains and scaffold properties for existing viral targets, and is well suited to quickly adapt bioluminescent sensor proteins to effectively detect newly evolving viral epitopes.
Karyotype instability varies by species and genotype combination in allohexaploidBrassica
Authors: Quezada-Martinez Daniela , Batley Jacqueline , Mason Annaliese S. 
Published: September 2023

AbstractSyntheticBrassicaallohexaploids (2n = AABBCC) do not exist naturally but can be produced between six different parent species combinations, and can be used to investigate processes of polyploid formation and genome stabilization. In this study, we investigated hybridization potential, accumulation and frequency of copy number variants (CNVs), fertility, and karyotype stability in advanced generations of diverse allohexaploid genotypes belonging to differentBrassicaallohexaploid species combinations (NCJ types:B. napus×B. carinata×B. juncea; junleracea types:B. juncea×B. oleracea; naponigra types:B. napus×B. nigra; and carirapa types:B. rapa×B. carinata). Only 3.2% of allohexaploid plants investigated were euploids, with high frequencies of rearrangements. Significant differences between genotypes and between lineages within parent genotype combinations were found for frequencies of euploids and rearrangements, with one NCJ line showing relatively high karyotype stability. Hybridization between different allohexaploids was mostly achievable, with 0 - 4.6 seeds per flower bud on average, and with strong effects of maternal genotype. Novel hybrids between allohexaploid lineages showed similar fertility and stability to their parents. In the novel hybrid population, a significant correlation was observed between the inheritance of A-genome chromosome fragments (relative to C-genome fragments) and the total number of seeds produced per plant (r= 0.24). Our results suggest that syntheticBrassicaallohexaploids can develop genomic stability, but that this occurs at very low frequencies, and may not always be under selective pressure due to the unpredictable relationship between fertility and genome composition in these hybrid types.Article SummaryBrassicaplants with three sets of chromosomes (allohexaploids) do not exist in nature, but can be made from combinations between six different species. Here, we compared different combinations to see which are the most genomically stable and fertile, and found major differences between all allohexaploid types as well as one putatively stable line. Crosses between allohexaploid types could also be achieved in most cases, although hybrids were not more stable or fertile than their parents.
Counter-accounting and social transformation: Yaṉangu way
Authors: Norris Ellie , Kutubi Shawgat , Greenland Steven , Wallace Ruth 
Published: September 2023

PurposeThis study explores citizen activism in the articulation of a politicised counter-account of Aboriginal rights. It aims to uncover the enabling factors for a successful challenge to established political norms and the obstacles to the fullest expression of a radical imagining.Design/methodology/approachLaclau and Mouffe's theory of hegemony and discourse is used to frame the movement's success in challenging the prevailing system of urbanised healthcare delivery. Empirical materials were collected through extensive ethnographic fieldwork.FindingsThe findings from this longitudinal study identify the factors that predominantly influence the transformational success of an Yaṉangu social movement, such as the institutionalisation of group identity, articulation of a discourse connected to Aboriginal rights to self-determination, demonstration of an alternative imaginary and creation of strong external alliances.Originality/valueThis study offers a rich empirical analysis of counter-accounting in action, drawing on Aboriginal governance traditions of non-confrontational discourse and collective accountability to conceptualise agonistic engagement. These findings contribute to the practical and theoretical construction of democratic accounting and successful citizen activism.
Corrosion property of Al alloys subjected to surface severe plastic deformation: a review
Authors: Chen Jiabo , Guo Xiaokai , Liu Hao , Lv Xuantong , Fan Shichuan , Wu Liankui , Cao Fahe , Sun Qingqing 
Published: September 2023

Purpose
This study aims to discuss the influences of surface severe plastic deformation (S2PD) on the electrochemical corrosion, pitting corrosion, intergranular corrosion, stress corrosion cracking of aluminum (Al) alloys and attempt to correlate the microstructural/compositional changes with the performances.

Design/methodology/approach
This study provides a novel gradient design of structure/composition caused by S2PD for the purpose of enhancing Al alloys’ corrosion resistance.

Findings
S2PD has a significant effect on corrosion behavior of Al alloys through tuning the grain size, residual stress, composition, grain boundary phase and second phase particle distribution.

Originality/value
Although Al alloys are known to form a protective Al2O3 film, corrosion is a major challenge for the longevity of Al structures across numerous industries, especially for the infrastructures made of high-strength Al alloys. Traditional strategies of improving corrosion resistance of Al alloys heavily relied on alloying and coatings. In this review, gradient design of structure/composition caused by S2PD provides a novel strategy for corrosion protection of Al alloys, especially in the enhancement of localized corrosion resistance.
Who generates your video ads? The matching effect of short-form video sources and destination types on visit intention
Authors: Li Hui , Tu Xinxin 
Published: September 2023

PurposeShort-form videos have gradually become important marketing tools for tourist destinations. However, chaotic sources and homogenized content have led to poor user experiences. Taking Kulangsu and Xi'an City Wall in China as examples, this study explored the influence of matching short-form video sources with destination types on user engagement and visit intention.Design/methodology/approachThis study selected three short-form videos from different sources for each destination on TikTok and conducted an empirical research using a 3 × 2 experimental design to examine the proposed research model.FindingsThe results showed that the matching effect between short-form video sources and destination types will positively affect user engagement and visit intention. (1) the short-form videos with user-generated content (UGC) or professional user-generated content (PUGC) in hedonic destinations can obtain higher user engagement and visit intention; (2) the short-form videos with professionally generated content (PGC) or PUGC in utilitarian destinations can obtain higher user engagement and visit intention and (3) perceived credibility and perceived usefulness played mediating roles in these interactions.Originality/valueThis study considers short-form video sources as antecedent variables influencing user engagement and visit intention and confirms the matching effect between short-form video sources and tourism destination types. The findings will help researchers and marketers better understand the impact of short-form video on destination marketing.
The relationship between career satisfaction and organizational commitment: evidence from the Kuwaiti banking sector
Authors: Baidoun Nizar , Anderson Valerie Anne 
Published: September 2023

Purpose
Grounded in social cognitive career theory (SCCT), this study aims to examine the influence of contextual factors on the relationship between career satisfaction and organizational commitment, within the banking sector in Kuwait.

Design/methodology/approach
This study used a cross-sectional design analyzing a self-report questionnaire (N = 278).

Findings
This study investigates affective, normative and continuance commitment in relation to career satisfaction, within the banking sector in Kuwait. Findings indicate a positive relationship between career satisfaction and all of affective, normative and continuance commitment; although the relationship that appears to be the strongest is between career satisfaction and normative commitment.

Research limitations/implications
The single site, cross-sectional approach is a limitation. The data were collected before the COVID-19 pandemic. Future research into career satisfaction and organizational commitment in different sectors is necessary and a replication of this study in a post-COVID context would also be valuable.

Practical implications
Human resource development (HRD) policies in contexts such as Kuwait should prioritize career progression initiatives to enhance career satisfaction and contribute to increased organizational commitment. More attention is necessary to organizational HRD career planning and development policies and processes. Effective line manager development programs to equip managers to provide feedback and constructive performance management are recommended, as is the organizational provision of career counseling and guidance to support career development policies and processes.

Originality/value
This study combines the use of established constructs with an SCCT theoretical lends to contribute new theorization of the relationship between career satisfaction and organizational commitment in non-Western cultural contexts. It challenges assumptions in current theorization of the relationship between career satisfaction and commitment that privilege affective commitment over other dimensions.
Impact of socioeconomic prosperity and race on crowdfunding success
Authors: Koh Yoon , Mao-Clark Xiaodan , DeFranco Agnes 
Published: September 2023

Purpose
Prior research treated entrepreneurs’ actions as purely opportunistic and voluntary, excluding social and economic systems’ influence on entrepreneurial actions. However, the applications of communication strategies, project management and social network are anchored in socioeconomic systems in which the entrepreneurs are rooted. To address the gap, this study aims to articulate – through the prism of institutional theory – how restaurant crowdfunding (CF) success is affected by socioeconomic prosperity according to entrepreneurs’ race and geographic area.

Design/methodology/approach
The current study analyzed 2,008 restaurant CF projects launched in the USA through the Kickstarter platform from 2010 to 2020. By conducting one-way analysis of variance and multilevel mixed-effect logistic regression models, this study examined the relative socioeconomic prosperity and CF success according to the race of the restaurant entrepreneurs. The study also examined how socioeconomic prosperity affected CF success and how that relationship was moderated by the entrepreneurs’ level of restaurant experience.

Findings
This study finds that relative socioeconomic prosperity and CF success does differ according to race. Also in the CF context, lower socioeconomic prosperity does impede fundraising success. While the level of restaurant experience significantly increased an entrepreneur’s CF success, the impact was not so significant as to overcome the impact of socioeconomic prosperity.

Research limitations/implications
Drawing on institutional theory, this study examines the impact of socioeconomic prosperity on CF project outcomes. By uncovering the significant impact of socioeconomic systems on CF success, this study fills the research gap. Previous studies have generally treated minority entrepreneurs as an aggregated form. The authors’ results extend the literature by including major ethnic groups – whites, African Americans and Asians.

Practical implications
The findings of the current study show restaurant entrepreneurs can raise the likelihood of CF success by doing two things: first, accumulate experience in the restaurant industry; second, use their CF websites to highlight testimonials about the value of that experience. Federal, state and local governments can institute policies to help improve racial minorities’ socioeconomic conditions and thereby promote startups’ fundraising success.

Originality/value
To the best of the authors’ knowledge, this study is one of the first to examine unexplored institutional effect on CF outcomes. It examines how and why socioeconomic factors affect minority entrepreneurs’ funding success. It compares the prosperity and CF success of white, African American and Asian entrepreneurs.
What are the factors of parental incarceration that may increase risk of poor emotional and mental health in children of prisoners?
Authors: Thorne Matthew Charles , de Viggiani Nick , Plugge Emma 
Published: September 2023

Purpose
Globally millions of children have a parent who is imprisoned. Research suggests that this has an adverse impact on the child and imprisonment of a parent is considered to be an adverse childhood experience (ACE). Parental incarceration will not only affect the child but the entire household and may result in further ACEs such as household dysfunction and parental separation making this group of children particularly vulnerable. This scoping review aims to adopt an international perspective to comprehensively examine the extent range and nature of literature both published and grey relating to parental incarceration and the potential impact on children’s emotional and mental health.

Design/methodology/approach
In this scoping review, the five stages identified by Arksey and O’Malley (2005) were used including identifying the research question, identifying relevant studies, study selection, charting data, collating, summarising and reporting results. In addition, the included studies were appraised for quality using methodology-specific tools. A critical narrative synthesis was adopted to present findings and discussion.

Findings
Nine studies met the inclusion criteria. Of the included studies, eight were retrieved from peer-reviewed journals and one from grey literature searching. Five categories with subcategories were identified affecting children’s mental health: 1) Relationships: parent and incarcerated child relationship; facilitators and barriers to maintaining contact; 2) Family structure; maternal or paternal incarceration; living arrangements during parental incarceration; 3) Children’s emotions: emotional recognition and regulation; resilience; 4) Prison stigma: social stigma; shame and secrecy; 5) Structural disadvantages: poverty; race/ethnicity.

Originality/value
This scoping review has highlighted how the imprisonment of a parent negatively affects their children’s emotional and mental health. Factors negatively impacting children’s emotional and mental health are interrelated and complex. Further research is required, including differences between paternal and maternal incarceration; impact of gender and age of child; poverty as an ACE and prison exacerbating this; and effects of ethnicity and race. An important policy direction is in developing an effective way of capturing the parental status of a prisoner to ensure that the child and family receive needed support.
Understanding young Chinese customers’ perceptions of unsafe city image restoration after Covid-19 outbreak
Authors: Li Chang (Cherise) , Agyeiwaah Elizabeth , Imboden Alain , Lee Younghee Maria 
Published: September 2023

Purpose
This study aims to uncover marketing strategies to restore a positive image in times of pandemic crisis to bring tourists back to a popularly affected tourism city in China, Wuhan in Hubei Province. The paper argues that the process of restoring city image requires understanding the perceptions of Generation Z, a segment of the population who have a high propensity to travel after COVID-19.

Design/methodology/approach
This study employed a constructivist grounded theory approach to explore the perspectives of China’s Generation Z consumers of Wuhan’s future image to restart tourism. The data is collected through semi-structured interview of 29 respondents and analyzed with the initial, focused and theoretical coding process.

Findings
Start by narrowing the gap between projected and perceived image, the findings suggest that Generation Z could be pulled to visit Wuhan city through four core products such as dark tourism activities, special-interest leisure, heroism and storytelling.

Originality/value
The destination image restoration framework after health-related crisis is creatively proposed. It combines the analysis of crisis and urban characteristics from the perspective of the target audience (Generation Z) and provides specific strategies to restore the tourism city image from cognitive, affective and conative dimensions. Significantly, two novel characteristics of Generation Z (i.e. lighthearted and patriotic) emerge in addition to the typical features of this generational cohort. This study also found a high preference for a technologically oriented type of attraction that reverses the morbid COVID-19 memories into an entertainment tool.