bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incorporating nanoconfined chitin‐fibrils in poly (ε‐caprolactone) membrane scaffolds improves mechanical and chemical properties for biomedical application
Authors: Saudi Sheikh , Jun Sunghyun , Fialkova Svitlana , Surendran Vikram , Chandrasekaran Arvind , Bhattarai Shanta R. , Sankar Jagannathan , Bhattarai Narayan 
Published: January 2023

AbstractEngineered composite scaffolds composed of natural and synthetic polymers exhibit cooperation at the molecular level that closely mimics tissue extracellular matrix's (ECM) physical and chemical characteristics. However, due to the lack of smooth intermix capability of natural and synthetic materials in the solution phase, bio‐inspired composite material development has been quite challenged. In this research, we introduced new bio‐inspired material blending techniques to fabricate nanofibrous composite scaffolds of chitin nanofibrils (CNF), a natural hydrophilic biomaterial and poly (ɛ‐caprolactone) (PCL), a synthetic hydrophobic‐biopolymer. CNF was first prepared by acid hydrolysis technique and dispersed in trifluoroethanol (TFE); and second, PCL was dissolved in TFE and mixed with the chitin solution in different ratios. Electrospinning and spin‐coating technology were used to form nanofibrous mesh and films, respectively. Physicochemical properties, such as mechanical strength, and cellular compatibility, and structural parameters, such as morphology, and crystallinity, were determined. Toward the potential use of this composite materials as a support membrane in blood–brain barrier application (BBB), human umbilical vein endothelial cells (HUVECs) were cultured, and transendothelial electrical resistance (TEER) was measured. Experimental results of the composite materials with PCL/CNF ratios from 100/00 to 25/75 showed good uniformity in fiber morphology and suitable mechanical properties. They retained the excellent ECM‐like properties that mimic synthetic‐bio‐interface that has potential application in biomedical fields, particularly tissue engineering and BBB applications.
Advanced Glycation End Products and Bone Metabolism in Patients with Chronic Kidney Disease
Authors: Quadros Kélcia R. S. , Roza Noemi A. V. , França Renata A. , Esteves André B. A. , Barreto Joaquim , Dominguez Wagner V. , Furukawa Luzia N. S. , Caramori Jacqueline Teixeira , Sposito Andrei C. , de Oliveira Rodrigo Bueno 
Published: January 2023

ABSTRACTAdvanced glycation end products (AGEs) accumulation may be involved in the progression of CKD‐bone disorders. We sought to determine the relationship between AGEs measured in the blood, skin, and bone with histomorphometry parameters, bone protein, gene expression, and serum biomarkers of bone metabolism in patients with CKD stages 3 to 5D patients. Serum levels of AGEs were estimated by pentosidine, glycated hemoglobin (A1c), and N‐carboxymethyl lysine (CML). The accumulation of AGEs in the skin was estimated from skin autofluorescence (SAF). Bone AGEs accumulation and multiligand receptor for AGEs (RAGEs) expression were evaluated by immunohistochemistry; bone samples were used to evaluate protein and gene expression and histomorphometric analysis. Data are from 86 patients (age: 51 ± 13 years; 60 [70%] on dialysis). Median serum levels of pentosidine, CML, A1c, and SAF were 71.6 pmol/mL, 15.2 ng/mL, 5.4%, and 3.05 arbitrary units, respectively. AGEs covered 3.92% of trabecular bone and 5.42% of the cortical bone surface, whereas RAGEs were expressed in 0.7% and 0.83% of trabecular and cortical bone surfaces, respectively. AGEs accumulation in bone was inversely related to serum receptor activator of NF‐κB ligand/parathyroid hormone (PTH) ratio (R = −0.25; p = 0.03), and RAGE expression was negatively related to serum tartrate‐resistant acid phosphatase‐5b/PTH (R = −0.31; p = 0.01). Patients with higher AGEs accumulation presented decreased bone protein expression (sclerostin [1.96 (0.11–40.3) vs. 89.3 (2.88–401) ng/mg; p = 0.004]; Dickkopf‐related protein 1 [0.064 (0.03–0.46) vs. 1.36 (0.39–5.87) ng/mg; p = 0.0001]; FGF‐23 [1.07 (0.4–32.6) vs. 44.1 (6–162) ng/mg; p = 0.01]; and osteoprotegerin [0.16 (0.08–2.4) vs. 6.5 (1.1–23.7) ng/mg; p = 0.001]), upregulation of the p53 gene, and downregulation of Dickkopf‐1 gene expression. Patients with high serum A1c levels presented greater cortical porosity and Mlt and reduced osteoblast surface/bone surface, eroded surface/bone surface, osteoclast surface/bone surface, mineral apposition rate, and adjusted area. Cortical thickness was negatively correlated with serum A1c (R = −0.28; p = 0.02) and pentosidine levels (R = −0.27; p = 0.02). AGEs accumulation in the bone of CKD patients was related to decreased bone protein expression, gene expression changes, and increased skeletal resistance to PTH; A1c and pentosidine levels were related to decreased cortical thickness; and A1c levels were related to increased cortical porosity and Mlt. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Ab initio calculations of the interaction potentials and thermodynamic functions for ArN and ArN+
Authors: Maltsev Maxim A. , Aksenova Svetlana A. , Morozov Igor V. , Minenkov Yury , Osina Evgenia L. 
Published: January 2023

AbstractArgon compounds play an important role in the mass spectrometry with inductively coupled plasma and other applications. At the same time, there is a little knowledge of their electronic terms and thermodynamic functions due to the complexity of experimental observations. In this work, the ab initio simulations are performed to obtain the interatomic interaction potentials for the ground and excited states of ArN and ArN+. Using these potentials, the vibrational‐rotational partition functions and thermodynamic properties in the gas phase are calculated for these molecules at the temperature range of 298.15–10,000 K. The errors of the thermodynamic functions associated with the approximation of interatomic interaction potentials are estimated.
Understanding the mechanism and regio‐ and stereo selectivity of [3 + 2] cycloaddition reactions between substituted azomethine ylide and 3,3,3‐trifluoro‐1‐nitroprop‐1‐ene, within the molecular electron density theory
Authors: Sobhi Chafia , Merzoud Lynda , Bouasla Souad , Nacereddine Abdelmalek Khorief , Morell Christophe , Chermette Henry 
Published: January 2023

AbstractThe selectivity and the nature of the molecular mechanism of the [3 + 2] cycloaddition (32CA) reaction between 2‐(dimethylamino)‐1H‐indene‐1,3(2H)‐dione (AY11) and trans(E)‐3,3,3‐trifluoro‐1‐nitroprop‐1‐ene(FNP10) has been studied, in which the molecular electron density theory using density functional theory methods at the MPWB1K/6‐31G(d) computational level was used. Analysis of the global reactivity indices permits us to characterizeFNP10as a strong electrophile andAY11as a strong nucleophile. Four reactive pathways associated with the ortho/meta regioselective channels and endo/exo stereoselective approaches modes have been explored and characterized in the gas phase and in the benzene solvent. The analysis of the relative energies associated with the different reaction pathways indicates that the 32CA reactions of the azomethine ylide (AY)with the nitroalkene (FNP) is meta regioselective with high endo stereoselectivity. This result is in good agreement with the experimental observations. electron localization function topological analysis of the most favored reactive pathways allows for characterizing the mechanism of this 32CA reactions as a non‐concerted two‐stage one‐step mechanism. Finally, non‐covalent interactions and quantum theory of atoms in molecule analyses at the meta/endo transition state structure indicate that the presence of different several weak interactions, namely, CF and NH contributed in favoring the formation of a meta‐endo cycloadduct.
Association of handgrip strength and/or walking pace with incident chronic kidney disease: A UK biobank observational study
Authors: He Panpan , Ye Ziliang , Liu Mengyi , Li Huan , Zhang Yuanyuan , Zhou Chun , Wu Qimeng , Zhang Yanjun , Yang Sisi , Liu Chengzhang , Qin Xianhui 
Published: January 2023

AbstractBackgroundThe individual and combined relations of handgrip strength and walking pace with the risk of chronic kidney disease (CKD) remain uncertain. We aimed to investigate the relationship of handgrip strength and/or walking pace with incident CKD, using data from the large‐scale, observational UK Biobank.MethodsA total of 417 504 participants free of prior kidney diseases were included from UK Biobank. Handgrip strength was assessed by dynamometer. The walking pace was self‐reported as slow, average, or brisk. Cox proportional hazards models were applied to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) for incident CKD.ResultsThe average age of the study population was 56.3 (SD, 8.1) years. 192 012 (46.0%) of the participants were male. The mean handgrip strength was 23.5 (SD, 6.2) and 40.0 (SD, 8.8) kg for females and males, respectively. Over a median follow‐up duration of 12.1 years, 11 064 (2.7%) participants developed incident CKD. Handgrip strength was significantly inversely associated with the risk of incident CKD in both males and females (both P for trend <0.001). When handgrip strength was assessed as sex‐specific quartiles, compared with those in the first quartile, the adjusted HRs (95% CI) of incident CKD in participants in the second, third and fourth quartiles were 0.84 (0.79, 0.89), 0.76 (0.71, 0.81) and 0.72 (0.67, 0.77), respectively. Compared with those with slow walking pace, participants with average (HR, 0.64; 95% CI: 0.60–0.68) or brisk (HR, 0.53; 95% CI: 0.49–0.57) walking pace had significantly lower risks of incident CKD. Compared with those with both lower handgrip strength (the first quartile) and slow walking pace, the lowest risk of incident CKD was observed in participants with both higher handgrip strength (the 2–4 quartiles) and average or brisk walking pace (HR, 0.51; 95% CI: 0.46–0.55).ConclusionsHandgrip strength and walking pace were significantly inversely associated with incident CKD in the general population.
RETRACTED: The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress‐related quality of life in adult subjects: A randomized controlled clinical trial
Authors: Trivedi Mahendra Kumar , Branton Alice , Trivedi Dahryn , Mondal Sambhu , Jana Snehasis 
Published: January 2023

AbstractBackgroundWestern and Eastern cultures have practiced biofield energy therapy for thousands of years, but empirical research on effectiveness of this therapy is relatively nascent. Study was aimed to assess the safety and efficacy of biofield therapy on psychological symptoms and mental health disorders in adult subjects.MethodsSeventy‐seven participants (39 male and 38 female) underwent clinical trial. This trial was simple randomized, placebo‐controlled, parallel‐group, open‐label, and single‐center with subjects, who have one or more psychological symptoms. Two sessions of biofield energy attunement were given in‐person at day 0 and 90 for 3 min (treatment group, n = 35) and others allocated to naive attunement (placebo group, n = 42). Subjects were assessed psychological questionnaire scoring using standard scale of assessment and levels of physiological biomarkers in serum were determined by parameter‐specific ELISA.ResultsPerceived psychological symptoms/scores (asthenia, sleep disturbances, anxiety, depression, stress, confusion, future fearness, sexual desireness, motivation, confidence, emotional trauma, etc.) were significantly (p ≤ 0.0001) improved in the treatment group than placebo control group. Furthermore, physiological biomarkers: vitamins (B12, C, and D3 metabolites), immune biomarker (CD8+CD28−), neurotransmitters (acetylcholine, noradrenaline, and dopamine), hormones (oxytocin, 17‐β‐estradiol, and insulin), and antiaging protein (klotho) levels were significantly (p ≤ 0.001) increased in treatment group than placebo. Proinflammatory cytokines (TNF‐α, IL‐1β, IL‐6, and IL‐8) and oxidative stress biomarkers (isoprostane and oxidized LDL) were reduced in treatment group compared with placebo.ConclusionsResults suggest that experimenter's biofield energy plays a significant role in information transfer processes that contribute to individual's state of physical, mental, emotional, and spiritual well‐being as well as improved overall health and quality of life.
Pathological survey of precursor lesions in cholangiocarcinoma
Authors: Nakanuma Yasuni , Sugino Takashi , Kakuda Yuko , Nomura Yoshikatsu , Watanabe Hiroyuki , Terada Takuro , Sato Yasunori , Ohnishi Yoshifumi , Fukumura Yuki 
Published: January 2023

AbstractBackgroundTo clarify the pathological significance of two precursors (high‐grade biliary intraepithelial neoplasm [BilIN] and intraductal papillary neoplasm of bile duct [IPNB]) in cholangiocarcinomas (CCAs).MethodsNinety‐one cases of CCA (47 distal CCAs [dCCAs], 31 perihilar CCAs [pCCAs] and 13 intrahepatic CCAs of large duct type [LD‐iCCAs]) were examined for their association with precursors. Neoplastic intraepithelial lesions without underlying infiltrating carcinoma in the surrounding mucosa of CCAs were considered to reflect high‐grade BilIN. High‐grade BilIN and IPNB were subdivided into gastric, biliary, intestinal and oncocytic subtypes, while CCAs were subdivided into gastrobiliary, intestinal and oncocytic subtypes. The postoperative overall survival (OS) was examined.ResultsFifty‐four and 8 of 91 CCAs were associated with high‐grade BilIN and IPNB, respectively, while these precursors were unidentifiable in the remaining CCAs. A majority of CCAs were of the gastrobiliary subtype, while the intestinal subtype was occasionally detected, and the oncocytic subtype was rare. CCAs with high‐grade BilIN showed a similar postoperative OS to CCAs without precursors, while CCAs with IPNB showed a favorable postoperative OS compared to CCAs without precursors.ConclusionsCCAs were frequently associated with precursors; high‐grade BilIN may be a major precursor and IPNB a minor one. CCAs with IPNB showed a favorable postoperative OS compared to CCAs with high‐grade BilIN.
Impact of very early introduction of everolimus on liver regeneration after partial liver transplantation in rats
Authors: Hirata Masaaki , Yagi Shintaro , Ito Takashi , Masano Yuki , Miyachi Yosuke , Yao Siyuan , Sonoda Mari , Masuda Satohiro , Haga Hironori , Hatano Etsuro 
Published: January 2023

AbstractBackground/PurposeThis experimental study in rats aimed to investigate the impact of very early introduction (within 3 h) of everolimus (EVR) + reduced‐tacrolimus (TAC) after partial liver transplantation (LT) on liver regeneration, rejection, and survival.MethodsBased on appropriate dose of EVR + reduced‐TAC in 70% hepatectomy (Experiment 1), allogeneic 30% partial LT (Experiment 2) and whole LT (Experiment 3) were performed.ResultsAfter partial LT in EVR + reduced‐TAC therapy, restoration of liver graft weight (to that of the whole liver) was delayed compared with standard dose TAC monotherapy (standard‐TAC) on day 3 (59.3% vs. 72.9%; p < .001) and 14 (88.1% vs. 95.5%; p = .01). Survival was 75%, which was not as high as the value of 100% observed for standard‐TAC, because neither infection nor rejection could be prevented. By contrast, survival after whole LT was 100% as neither infection nor rejection occurred.ConclusionsThe very early introduction of EVR + reduced‐TAC after partial LT delayed liver regeneration, and made it difficult to manage the dose required to suppress both infection and rejection. On the other hand, EVR + reduced‐TAC could be introduced safely very early after whole LT.
Molecular evolution of the human monkeypox virus
Authors: Wolf Jonas Michel , Wolf Lucas Michel , Fagundes Pamela Pereira , Tomm Dalila Mabel Schmidt , Petek Helena , Brenner Aline , Maccari Juçara Gasparetto , Nasi Luiz Antonio 
Published: January 2023

AbstractRecently, in 2022, new cases of human monkeypox virus (hMPXV) occurred in Europe and North America. The first case was reported in Europe in May 2022, and subsequently, more than 50 000 new cases were confirmed in 100 countries. Currently, the classification of hMPXV according to the nextstrain occurs in five big clades (1A, A.1, A.2, A.1.1, and B.1). According to the resurgence of smallpox‐like disease caused by hMPXV and the spread of the virus to the European and American continents, in the present study, we review and summarize the molecular evolution of the hMPXV, determining the molecular evolution of the main clades. A total of 442 hMPXV whole‐genome sequences with available information from the country and sampling date (between October 2017 and 2022), were obtained and evaluated using the Bayesian method. The clade B.1 which is currently circulating was the most frequent (n = 415; 93.9%). The other clades presented the following frequencies: 1A (n = 13; 2.9%), A.1 (n = 10; 2.3%), A.2 (n = 3; 0.7%) and A.1.1 (n = 1; 0.2%) The overall nucleotide divergence of hMPXV was 5.590e‐5. The 1A clade was detected between 2017 and 2020. A.1 was observed, and between 2019 and 2022 some A.2 sequences were detected. In 2022, the great predominance of B.1 was observed. The common ancestor of the hMPXV belongs to the clade 1A and the time to the Most Recent Common Ancestor (tMRCA) was 2017‐04‐04 (Highest Posterior Density 95% (HPD95%): 2017‐03‐09; 2017‐08‐04) on the West African continent. The tMRCA of A.1 was 2018‐05‐21 (HPD95%: 2018‐05‐20; 2018‐07‐04) with divergence of 6.885e‐5 substitutions per site per year. This clade was of West African origin but was eventually detected in European countries. Also, A.2 was detected with sequences of North America and showed tMRCA of 2019‐07‐15 (HPD95%: 2018‐11‐18; 2020‐02‐24). A.1.1 showed tMRCA from 2021 to 06‐05 (HPD95%: 2021‐06‐05; 2021‐11‐26) and this clade was detected in North America and was the precursor for the globally spreading B.1 which tMRCA was 2022‐04‐26 (HPD95%: 2022‐02‐27; 2022‐04‐26). hMPXV has been spread from West Africa to the United Kingdom, Israel, Singapore, the USA, Canada, Portugal, Spain, Ireland, France, Belgium, the Netherlands, Switzerland, Germany, Italy, Slovenia, Austria, the Republic Czech, Sweden, and Finland. hMPXV also reached countries such as Brazil, Mexico, Australia, and Taiwan. The common ancestor of the hMPXV belongs to the clade 1A with origin in the West African continent. Clade B.1 was responsible for the recent widespread worldwide. Immunization to prevent the spread of hMPXV is not yet available to the public, future studies should focus on the development of effective vaccines to contain the spread of this virus.
Transmission of monkeypox/mpox virus: A narrative review of environmental, viral, host, and population factors in relation to the 2022 international outbreak
Authors: Pan Daniel , Nazareth Joshua , Sze Shirley , Martin Christopher A. , Decker Jonathan , Fletcher Eve , Déirdre Hollingsworth T. , Barer Michael R. , Pareek Manish , Tang Julian W. 
Published: January 2023

AbstractMonkeypox virus (MPXV) has spread globally. Emerging studies have now provided evidence regarding MPXV transmission, that can inform rational evidence‐based policies and reduce misinformation on this topic. We aimed to review the evidence on transmission of the virus. Real‐world studies have isolated viable viruses from high‐touch surfaces for as long as 15 days. Strong evidence suggests that the current circulating monkeypox (mpox) has evolved from previous outbreaks outside of Africa, but it is yet unknown whether these mutations may lead to an inherently increased infectivity of the virus. Strong evidence also suggests that the main route of current MPXV transmission is sexual; through either close contact or directly, with detection of culturable virus in saliva, nasopharynx, and sperm for prolonged periods and the presence of rashes mainly in genital areas. The milder clinical presentations and the potential presence of presymptomatic transmission in the current circulating variant compared to previous clades, as well as the dominance of spread amongst men who have sex with men (MSMs) suggests that mpox has a developed distinct clinical phenotype that has increased its transmissibility. Increased public awareness of MPXV transmission modalities may lead to earlier detection of the spillover of new cases into other groups.
Ultrastructural analysis of monkeypox virus replication in Vero cells
Authors: Witt Amanda Stéphanie Arantes , Trindade Giliane de Souza , Souza Fernanda Gil de , Serafim Mateus Sá Magalhães , da Costa Alana Vitor Barbosa , Silva Marcos Vinícius Ferreira , de Melo Iani Felipe Campos , Rodrigues Rodrigo Araújo Lima , Kroon Erna Geessien , Abrahão Jônatas Santos 
Published: January 2023

AbstractIn early May 2022, the first worldwide monkeypox virus (MPXV) outbreak was reported, with different clinical aspects from previously studied human monkeypox infections. Despite monkeypox medical importance, much of its biological aspects remain to be further investigated. In the present work, we evaluated ultrastructural aspects of MPXV asynchronous infections in Vero cells by transmission electron microscopy (TEM). The viral strain was isolated from a male patient infected during the 2022 outbreak. TEM analysis showed: (i) adhered intracellular mature virus particles before entry of the host cell; (ii) a reorganization of the rough endoplasmic reticulum cisternae into the so‐called “mini‐nuclei” structure associated with genome replication; and (iii) noticeably different sites within the viral factory presenting granular or fibrillar aspects. We also observed viral crescents, different MPXV particle morphotypes, and cellular alterations induced by infection, such as changes in the cytoskeleton structure and multimembrane vesicles abundance. Taken together, to the best of our knowledge, these results revealed for the first‐time ultrastructural aspects of different steps of the MPXV cycle.
Production, purification, and characterization of inulinase from Streptomyces anulatus
Authors: Beroigui Oumaima , El ghadraoui Lahsen , Errachidi Faouzi 
Published: January 2023

AbstractInulinase is an enzyme that catalyzes inulin to d‐fructose. This enzyme can be extracted from plants, but it is difficult to obtain it in large quantities, so its production cost is high. Therefore, microbial inulinase has great potential for industrial needs. In the last decade, there have been very few reports on actinobacterial inulinases, especially on purification and characterization of inulinase process extraction. This study aims to select actinomycetes that possess high inulinase activity from the soil. To screen inulinase‐producing bacteria, modified Czapex‐Dox agar supplemented with 1% inulin powder was used. The most effective isolate was Streptomyces sp. EFBO8, morphological and genotypic identification methods, confirmed that the strain is Streptomyces anulatus and that its nucleotide sequence has been deposited in GenBank under accession number OQ073700. To optimize inulinase production, kinetics were performed by using S. anulatus strain, which proved to be most productive with a value of 24,024 EU/mL. The enzyme was purified from the culture filtrate by precipitation with ammonium sulfate (NH4)2SO4, followed by column chromatography Sephadex (G‐50) separation. Purified protein has a molecular mass of 3331.83 Da.