bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolution and emergence of mosquito-borne viruses of medical importance: towards a routine metagenomic surveillance approach
Authors: Laiton-Donato Katherine , Guzmán-Cardozo Camila , Peláez-Carvajal Dioselina , Ajami Nadim J. , Navas María-Cristina , Parra-Henao Gabriel , Usme-Ciro José A. 
Published: February 2023

Abstract
During the last two decades, the world has witnessed the emergence and re-emergence of arthropod-borne viruses, better known as arboviruses. The close contact between sylvatic, rural and peri-urban vector species and humans has been mainly determined by the environment-modifying human activity. The resulting interactions have led to multiple dead-end host infections and have allowed sylvatic arboviruses to eventually adapt to new vectors and hosts, contributing to the establishment of urban transmission cycles of some viruses with enormous epidemiologic impact. The metagenomic next-generation sequencing (NGS) approach has allowed obtaining unbiased sequence information of millions of DNA and RNA molecules from clinical and environmental samples. Robust bioinformatics tools have enabled the assembly of individual sequence reads into contigs and scaffolds partially or completely representing the genomes of the microorganisms and viruses being present in biological samples of clinical relevance. In this review, we describe the different ecological scenarios for the emergence of viral diseases, the virus adaptation process required for the establishment of a new transmission cycle and the usefulness of NGS and computational methods for the discovery and routine genomic surveillance of mosquito-borne viruses in their ecosystems.
Non-deep simple and deep simple morphophysiological dormancy in seeds of three species of Ilex from subtropical and tropical regions of Taiwan
Authors: Liu Chiung-Pin , Chen Shun-Ying , Baskin Carol C. , Chien Ching-Te 
Published: February 2023

AbstractDormancy-breaking requirements and level of morphophysiological dormancy (MPD) were determined for seeds of Ilex formosana and I. uraiensis from the subtropical region, and seeds of I. rotunda from both the subtropical and tropical regions of Taiwan. We hypothesized that some Ilex species would have deep simple MPD broken by warm stratification. Germination of seeds and embryo growth was monitored at 30/20, 25/15, 20/10, 15/5°C and at 25°C. Seeds were cold-stratified and then incubated at 25/15°C, and seeds treated with GA3 and GA4 were incubated at 25/15°C. Fresh seeds reached 50% germination after 11–45 weeks of warm stratification. Pre-treatment with GA increased germination percentages of I. formosana and I. rotunda (tropical) but not I. uraiensis and I. rotunda (subtropical), while cold stratification did not promote germination of either species but increased the germination rate of I. rotunda (tropical). Embryo length in seeds of all species increased ≥710% prior to root emergence, and growth occurred during warm stratification. The positive response to GA and relatively short time for beginning of germination and to reach 50% germination indicate non-deep simple MPD in seeds of I. formosana and I. rotunda (tropical). The negative response to GA and long time for beginning of germination and to reach 50% germination indicate deep simple MPD in seeds of I. uraiensis and I. rotunda (subtropical). Thus, in both the subtropical and tropical regions of Taiwan, the seeds of Ilex species have non-deep simple and deep simple MPD that are broken by warm stratification. Furthermore, GA treatment increases the germination rate and percentage of Ilex seeds with non-deep simple MPD, and cold stratification promotes the seed germination rate of Ilex species with non-deep simple MPD in tropical region.
Does neurocognition contribute to age-related deficits in the online navigation of electronic patient health portals?
Authors: Matchanova Anastasia , Babicz Michelle A. , Kordovski Victoria M. , Tierney Savanna M. , Rahman Samina , Medina Luis D. , Cushman Clint , Woods Steven Paul 
Published: February 2023

AbstractObjective:The internet serves an increasingly critical role in how older adults manage their personal health. Electronic patient portals, for example, provide a centralized platform for older adults to access lab results, manage prescriptions and appointments, and communicate with providers. This study examined whether neurocognition mediates the effect of older age on electronic patient portal navigation.Method:Forty-nine younger (18–35 years) and 35 older adults (50–75 years) completed the Test of Online Health Records Navigation (TOHRN), which is an experimenter-controlled website on which participants were asked to log-in, review laboratory results, read provider messages, and schedule an appointment. Participants also completed a neuropsychological battery, self-report questionnaires, and measures of health literacy and functional capacity.Results:Mediation analyses revealed a significant indirect effect of older age on lower TOHRN accuracy, which was fully mediated by the total cognitive composite.Conclusions:Findings indicate that neurocognition may help explain some of the variance in age-related difficulties navigating electronic patient health portals. Future studies might examine the possible benefits of both structural (e.g., human factors web design enhancement) and individual (e.g., training and compensation) cognitive supports to improve the navigability of electronic patient health portals for older adults.
Associations between sugar-sweetened beverages before and during pregnancy and offspring overweight/obesity in Japanese women: the TMM BirThree Cohort Study
Authors: Aizawa Misato , Murakami Keiko , Yonezawa Yudai , Takahashi Ippei , Onuma Tomomi , Noda Aoi , Ueno Fumihiko , Matsuzaki Fumiko , Ishikuro Mami , Obara Taku , Kuriyama Shinichi 
Published: February 2023

AbstractObjective:The association between high sugar-sweetened beverages (SSB) intake during pregnancy and offspring overweight/obesity has been reported only from Western countries. The objective of this study was to examine the association between SSB intake before and during pregnancy and offspring overweight/obesity among Japanese women.Design:Japanese prospective birth cohort study.Setting:We analysed mother–offspring pairs who participated in the Tohoku Medical Megabank Project Birth and Three-Generation Cohort Study from 2013 to 2017. SSB intake during pregnancy was evaluated using the FFQ and classified into three groups: none (0 g/d), medium (<195 g/d) and high (>195 g/d). Overweight or obesity at 1 year of age in offspring was defined as having a BMI Z-score greater than 2 sd, calculated based on the BMI reference data for Japanese children. Multiple logistic regression analyses were performed to examine the associations between SSB intake before and during pregnancy and offspring overweight/obesity, after adjusting for covariates.Participants:Japanese mother–offspring pairs (n 7114).Results:The overweight/obesity rate of the offspring was 8·8 %. Pregnant women with a high intake of SSB in early to mid-pregnancy had a higher risk of overweight/obesity in their offspring compared with those who did not; the OR was 1·52 (95 % CI (1·09, 2·12)).Conclusions:High SSB intake in early to mid-pregnancy was associated with an increased risk of offspring overweight/obesity at 1 year of age.
Developing and creating didactic proposals for Latin and Classical Culture Training course with the educational centre CEFIRE
Authors: Regagliolo Alberto 
Published: February 2023

AbstractThe aim of this paper is to set out the results of the Developing and creating didactic proposals for Latin and Classical Culture (now DPLCC) course, which was financed and organised with the Spanish Ministry of Education, CEFIRE, during 2021.The training programme is aimed at teachers and anyone qualified to teach classical languages. It has been divided into two parts: a theory and a practical section. The theory section entailed a review of some fundamental topics in language teaching. The practical section involved the creation of a didactic proposal based on the element of water. The course counted a total of 34 participants; only 22 finished the theory section and 12 the practical section. From a practical perspective for participants, it was observed that various activities were effectively carried out. In particular, the trainees produced quality texts adapted to the required level. In addition, the proposed exercises are diverse and appropriate for the planned objectives. On the other hand, the graphic design, page layout and illustrations proposed did not have a particular focus on quality, indicating a lesser degree of technical and graphic preparation for producing materials that are also visually appealing to a younger audience. The study shows that trainees revisit and create new activities from scratch, always staying aligned with the chosen theme and the proposed educational level.
Toward a Catchment‐Scale Assessment of Flood Peak Attenuation by Multiple Reservoirs
Authors: Cipollini S. , Volpi E. , Fiori A. 
Published: February 2023

AbstractThe estimation of reservoirs impact on flood peak reduction at the catchment scale is of fundamental importance for risk assessment and planning purposes. It is generally addressed using detailed hydrologic‐hydrodynamic simulations or simple, empirically based indices. The former provide detailed results, at the cost of a large amount of information and high computational efforts; the latter are cost‐effective and simple, yet they generally provide approximate results. A promising compromise between the two is the physically based attenuation index , based on the concept of equivalent reservoir; was recently proposed in the literature to estimate the impact of multiple reservoirs located in series along the main channel, based on the assumption of rectangular catchment. In this work, we extend the equivalent reservoir approach to a generic catchment, with any shape and without restrictions on the location of the reservoirs. For an effective assessment of the method, we also introduce the novel concept of Reservoir‐influenced Instantaneous Unit Hydrograph (RIUH); the RIUH can be derived using a Monte Carlo procedure to include the effects of reservoirs on the Instantaneous Unit Hydrograph. Simulations of numerous fictitious reservoir configurations in a real basin demonstrate the potential of the methods in reproducing the attenuation effect. Remarkably, an average error of 3%–5% exists between and the peak reduction of a less simplified reality (RIUH). Finally, we provide a global, catchment‐scale application of the attenuation index, which constitutes an effective tool for the evaluation of each reservoir in managed catchment system and the design or planning of new hydraulic infrastructures.
The oncogenic roles and clinical implications of YAP/TAZ in breast cancer
Authors: Luo Juan , Zou Hailin , Guo Yibo , Tong Tongyu , Chen Yun , Xiao Yunjun , Pan Yihang , Li Peng 
Published: February 2023

AbstractBreast cancer (BC) is the most commonly diagnosed form of cancer and a leading cause of cancer-related deaths among women worldwide. Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are homologous transcriptional coactivators and downstream effectors of Hippo signalling. YAP/TAZ activation has been revealed to play essential roles in multiple events of BC development, including tumour initiation, progression, metastasis, drug resistance and stemness regulations. In this review, we will first give an overview of YAP/TAZ-mediated oncogenesis in BC, and then systematically summarise the oncogenic roles of YAP/TAZ in various BC subtypes, BC stem cells (BCSCs) and tumour microenvironments (TMEs). Based on these findings, we will further discuss the clinical implications of YAP/TAZ-based targeted therapies in BC and the potential future direction.
Synergistic mechanism between the endoplasmic reticulum and mitochondria and their crosstalk with other organelles
Authors: Zhang Yaozhi , Wu Yang , Zhang Minjie , Li Zixian , Liu Bin , Liu Huafeng , Hao Junfeng , Li Xiaoyu 
Published: February 2023

AbstractOrganelles are functional areas where eukaryotic cells perform processes necessary for life. Each organelle performs specific functions; however, highly coordinated crosstalk occurs between them. Disorder of organelle networks often occur in various diseases. The endoplasmic reticulum (ER) and mitochondria are crucial organelles in eukaryotic cells as they are the material synthesis and oxidative metabolism centers, respectively. Homeostasis and orchestrated interactions are essential for maintaining the normal activities of cells. However, the mode and mechanism of organelle crosstalk is still a research challenge. Furthermore, the intricate association between organelle dyshomeostasis and the progression of many human diseases remains unclear. This paper systematically summarized the latest research advances in the synergistic mechanism between the endoplasmic reticulum and mitochondria and their crosstalk with other organelles based on recent literature. It also highlights the application potential of organelle homeostasis maintenance as a preventative and treatment strategy for diseases.
Benthic biota of Chilean fjords and channels in 25 years of cruises of the National Oceanographic Committee
Authors: Aldea Cristian , Novoa Leslie , Acuña María Paz , Acevedo-Romo Ignacia , Bravo Francisco 
Published: February 2023

AbstractThe CIMAR program (Marine Research Cruises to Remote Areas) run by the Chilean Navy through the National Oceanographic Committee has been developed since 1995, focused on Chilean fjord and channel zones (~41–56°S; “CIMAR-Fjords”) and Chilean remote islands (“CIMAR-Islands”). Samples and data was collected on biotic and abiotic variables on all these cruises, both from the water column and benthos. Our work standardizes, compiles, and summarizes the published information on benthic organisms for twenty-one CIMAR-Fjords cruises developed in the first 25 years of the program, plus the Southern Ice Fields Cruise 1995 (precursor of the CIMAR program), which includes the distribution, abundance and geographic location of cruises sampling stations. The data set includes 8,854 records from 880 different localities, corresponding to 1,225 species from 24 different phyla (four kingdoms) and more than 150,000 individuals. Only two cruises did not record any benthic sampling. The fjords and channels of Chilean Patagonia have high biodiversity, so we hope that our data set will serve as a baseline for ecological studies and ecosystem conservation.
Genetic population structures of common scavenging species near hydrothermal vents in the Okinawa Trough
Authors: Kise Hiroki , Iguchi Akira , Ikegami Takuji , Onishi Yosuke , Goto Koichi , Tanaka Yuichiro , Washburn Travis W. , Nishijima Miyuki , Kunishima Taiga , Okamoto Nobuyuki , Suzuki Atsushi 
Published: February 2023

AbstractDeep-sea mining of hydrothermal deposits off the coast of Japan is currently under consideration, and environmental baseline studies of the area are required to understand possible impacts. The aim of this study is to clarify population structures of dominant benthic megafaunal species near hydrothermal vent fields in the Okinawa Trough, using a population genetics approach. We examined dominant deep-sea scavenging species including eels, several amphipods, and a decapod and performed population genetic analyses based on the mitochondrial cytochrome c oxidase subunit I region. Several sites were sampled within Okinawa Trough to examine intra-population diversity while two other locations 1400–2400 km away were chosen for inter-population comparisons. For synaphobranchid eels Simenchelys parasitica and Synaphobranchus kaupii, our results showed significant intra-population diversity but no inter-population genetic differentiation, suggesting strong genetic connectivity and/or large population sizes. In addition, single nucleotide polymorphism analysis also confirmed strong genetic connectivity for Simenchelys parasitica. Among scavenging amphipods, we detected seven putative species using molecular phylogenetic analysis. We evaluated population structures of the most abundant species of amphipods and a decapod species (Nematocarcinus lanceopes). Our results provide basic information on the genetic population structures of benthic megafaunal species near hydrothermal vent fields, which can be used to select candidate species for future connectivity analysis with high-resolution genetic markers and aid understanding of the potential population impacts of environmental disturbances.
Experimental and clinical data analysis for identification of COVID-19 resistant ACE2 mutations
Authors: Raghav Pawan Kumar , Raghav Aditya , Lathwal Anjali , Saxena Archit , Mann Zoya , Sengar Manisha , Rajalingam Raja 
Published: February 2023

AbstractThe high magnitude zoonotic event has caused by Severe Acute Respitarory Syndrome CoronaVirus-2 (SARS-CoV-2) is Coronavirus Disease-2019 (COVID-19) epidemics. This disease has high rate of spreading than mortality in humans. The human receptor, Angiotensin-Converting Enzyme 2 (ACE2), is the leading target site for viral Spike-protein (S-protein) that function as binding ligands and are responsible for their entry in humans. The patients infected with COVID-19 with comorbidities, particularly cancer patients, have a severe effect or high mortality rate because of the suppressed immune system. Nevertheless, there might be a chance wherein cancer patients cannot be infected with SARS-CoV-2 because of mutations in the ACE2, which may be resistant to the spillover between species. This study aimed to determine the mutations in the sequence of the human ACE2 protein and its dissociation with SARS-CoV-2 that might be rejecting viral transmission. The in silico approaches were performed to identify the impact of SARS-CoV-2 S-protein with ACE2 mutations, validated experimentally, occurred in the patient, and reported in cell lines. The identified changes significantly affect SARS-CoV-2 S-protein interaction with ACE2, demonstrating the reduction in the binding affinity compared to SARS-CoV. The data presented in this study suggest ACE2 mutants have a higher and lower affinity with SARS-Cov-2 S-protein to the wild-type human ACE2 receptor. This study would likely be used to report SARS-CoV-2 resistant ACE2 mutations and can be used to design active peptide development to inactivate the viral spread of SARS-CoV-2 in humans.
Evaluation of RT-PCR assays for detection of SARS-CoV-2 variants of concern
Authors: Dip Sourav Dutta , Sarkar Shovon Lal , Setu Md. Ali Ahasan , Das Prosanto Kumar , Pramanik Md. Hasan Ali , Alam A. S. M. Rubayet Ul , Al-Emran Hassan M. , Hossain M. Anwar , Jahid Iqbal Kabir 
Published: February 2023

AbstractSevere Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) pandemic has been considered with great importance on correct screening procedure. The detection efficiency of recent variants of concern were observed by comparing 5 commercial RT-PCR kits and a SYBR-green method developed and validated in our laboratory. The RNA was extracted from nasopharyngeal samples from suspected COVID-19 patients and RT-PCR assay was performed according to the instruction of the respective manufacturers. The specificity and sensitivity of Maccura kit was 81.8% and 82.5%, A*Star kit was 100% and 75.4%, Da An Gene kit was 100% and 68.4%, Sansure kit was 54.5% and 91.2% and TaqPath kit was 100% and 70.2% respectively. Our in house SYBR-Green method showed a consistent detection result with 90.9% specificity and 91.2% sensitivity. We also found that detection kits targeting more genes showed better accuracy which facilitates less false positive results (< 20%). Our study found a significant difference (p < 0.005) in Ct value reported for common target genes shared by the RT-PCR kits in relation with different variants of COVID-19 infection. Recent variants of concerns contain more than 30 mutations in the spike proteins including 2 deletion and a unique insertion mutation by which makes detection of these variants difficult and these facilitates the variants to escape from being detected.
Interfacial interaction driven enhancement in the colossal magnetoresistance property of ultra-thin heterostructure of Pr0.6Sr0.4MnO3 in proximity with Pr0.5Ca0.5MnO3
Authors: Gayathri V. , Amaladass E. P. , Sathyanarayana A. T. , Geetha Kumary T. , Pandian R. , Gupta Pooja , Rai Sanjay K. , Mani Awadhesh 
Published: February 2023

AbstractThe ultra-thin heterostructure of Pr0.6Sr0.4MnO3(15 nm)/Pr0.5Ca0.5MnO3(15 nm)/SrTiO3 fabricated using pulsed laser deposition technique exhibits the phase-segregated nature wherein the ferromagnetism of Pr0.6Sr0.4MnO3, and the antiferromagnetic state of Pr0.5Ca0.5MnO3 coexist in proximity. The observation of two exciting phenomena in the grown ultra-thin heterostructure, namely, the kinetic arrest and training effect, confirms its phase-segregated nature. The melting of the antiferromagnetic state in Pr0.5Ca0.5MnO3 into a ferromagnetic state due to the interfacial interaction arising from the magnetic proximity of the ferromagnetic clusters of Pr0.6Sr0.4MnO3 have been observed. A metal–insulator transition (TMIT) found at 215 K, close to its Curie temperature (TCurie) observed at 230 K, reveals a strong correlation between the electrical transport and the magnetization of the ultra-thin heterostructure. The electrical conduction in the high-temperature regime is explained in terms of the adiabatic small polaron hopping model. While the resistance in the metallic regime for temperatures above 100 K is contributed by the inelastic scattering due to the two-magnons, in the metallic regime below 100 K, the one-magnon inelastic scattering contribution is prevalent. An enhanced colossal magnetoresistance property near room temperature is obtained in the ultra-thin heterostructure arising from the proximity-driven interfacial interaction, making it a suitable candidate for technological applications near room temperature.
Development of an artificial intelligence based model for predicting the euploidy of blastocysts in PGT-A treatments
Authors: Yuan Zhenya , Yuan Mu , Song Xuemei , Huang Xiaojie , Yan Weiqiao 
Published: February 2023

AbstractThe euploidy of embryos is unpredictable before transfer in in vitro fertilisation (IVF) treatments without pre-implantation genetic testing (PGT). Previous studies have suggested that morphokinetic characteristics using an artificial intelligence (AI)-based model in the time-lapse monitoring (TLM) system were correlated with the outcomes of frozen embryo transfer (FET), but the predictive effectiveness of the model for euploidy remains to be perfected. In this study, we combined morphokinetic characteristics, morphological characteristics of blastocysts, and clinical parameters of patients to build a model to predict the euploidy of blastocysts and live births in PGT for aneuploidy treatments. The model was effective in predicting euploidy (AUC = 0.879) but was ineffective in predicting live birth after FET. These results provide a potential method for the selection of embryos for IVF treatments with non-PGT.