bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regional differences in fishing behavior determine whether a marine reserve network enhances fishery yield
Authors: Lenihan Hunter S. , Reed Daniel C. , Vigo Maria , Leiphardt Callie , Hofmiester Jennifer K.K. , Gallagher Jordan P. , Voss Chris , Moore Peyton , Miller Robert J. 
Published: April 2023

Abstract
Model simulations show that a network of marine reserves can enhance yield in depleted fisheries by protecting populations, particularly large, old spawners that supply larvae for interspersed fishing grounds. The ability of marine reserves to enhance sustainable fisheries is much less evident. We report empirical evidence of a marine reserve network improving yield for a sustainable spiny lobster fishery, apparently through the spillover of adult lobsters and behavioral adaptation by the fishing fleet. Results of a Before-After, Control-Impact analysis found catch, effort, and Catch-Per-Unit Effort increased after the establishment of marine reserves in the northern region of the fishery where fishers responded by fishing intensively at reserve borders, but declined in the southern region where they vacated once productive fishing grounds. Our results highlight the value of collaborative research and education programs involving diverse stakeholders for preparing fisheries to operate productively within a seascape that includes a large marine reserve network.
Effect of low-frequency electrical acupoint stimulation on nausea and vomiting in NSCLC patients receiving highly emetogenic regimens
Authors: Zhang Lingling , Wei Xing , Wang Xiangyi , Lin Li , Tang Chuanhao , Liang Jun 
Published: April 2023

Abstract
Purpose: To assess the efficacy and tolerability of low-frequency electrical acupoint stimulation plus standard antiemetics following highly emetogenic chemotherapy in patients with non-small cell lung cancer (NSCLC).
Methods: NSCLC patients who received highly emetogenic chemotherapy were randomized into control and observation groups by random number table. Patients in the observation group received the triple antiemetic regime plus low-frequency electrical acupoint stimulation (since the day of chemotherapy for 5 days consecutively). Meanwhile, those in the observation group only received the triple antiemetic regime. The severity of nausea and vomiting episodes were recorded on a daily basis. On the sixth day after chemotherapy, the patients were scored by the Functional Living Index Emesis (FLIE) questionnaire. The complete response rate of vomiting, incidence of nausea, severity of nausea, and FLIE score were compared between the two groups.
Results: Compared with the control group, the incidence of nausea decreased significantly in the observation group throughout the course of treatment (66.7% vs. 40.5%, p=0.016). At 24-120h after chemotherapy, the complete response rate of vomiting at the delayed stage increased conspicuously in the observation group (88.1% vs. 69.1%, p=0.033). At this stage, the proportion of patients without nausea in the observation group was also significantly higher (73.8% vs. 47.6%, p=0.014). The total FLIE scores in the observation group were conspicuously higher than the control group (106.05±15.35 vs. 95.04±20.02, p=0.02).
Conclusion: The combination of the triple antiemetic regimen and low-frequency electrical acupoint stimulation memorably improves chemotherapy-induced nausea and vomiting in NSCLC patients.
Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin
Authors: Chun Taewoo , Pattem Jacob , Gillis Richard B. , Dinu Vlad T. , Yakubov Gleb E. , Corfield Anthony P. , Harding Stephen E. 
Published: April 2023

Abstract
Glycopeptide antibiotics are regularly used in ophthalmology to treat infections of Gram-positive bacteria. Aggregative interactions of antibiotics with mucins however can lead to long exposure and increases the risk of resistant species. This study focuses on the evaluation of potential interactions of the last line of defence glycopeptide antibiotic teicoplanin with an ocular mucin model using precision hydrodynamic and microscopic techniques: sedimentation velocity in the analytical ultracentrifuge (SV-AUC), dynamic light scattering (DLS) and atomic force microscopy (AFM). For the mixtures of teicoplanin at higher doses (1.25 mg/mL and 12.5 mg/mL), it was shown to interact and aggregate with BSM in the distributions of both sedimentation coefficients by SV-AUC and hydrodynamic radii by DLS. The presence of aggregates was confirmed by AFM for higher concentrations. We suggest that teicoplanin eye drop formulations should be delivered at concentrations of < 1.25 mg/mL to avoid potentially harmful aggregations and to reduce the risk of antimicrobial resistance
Molecular screening of the most common streptococci affecting Common pandora (Pagellus erythrinus Linnaeus, 1758) caught from the Mediterranean coast of Tripoli
Authors: Eissa Alaa Eldin , Asheg Abdulatif A. , Sharaf Mahmoud S. , Abdelbaky Awad A. , Mhara Abdelsalam Abu , Dakhil Tarek D. , Gaafar Alkhateib Y. , Ismail Eman M. , Behiri Said El , Afiffy Emad A. , Prince Abdelbary , Shahin Khalid 
Published: April 2023

Abstract
A total number of samples, 270 Common pandora (Pagellus erythrinus), were clinically examined for possible streptococcal infection. The fishes were collected from the area extending from Tripoli to Tajoura (east to Tripoli). S. iniae, S. dysgalactiae, S. phocae, Enterococcus fecalis, and other non-streptococcal spp. Such as Aeromonas hydrophila, Pseudomonas, and Photobacterium damselae were biochemically identified. 90 fish per season were thoroughly screened for streptococcal infection for three successive seasons. Regardless of the season, S. iniae and E. fecalis were the most isolated streptococcal spp (13% and 8.88%, respectively). In contrast, the most retrieved non-streptococcal spp were A. hydrophila, followed by Pseudomonas spp, with isolation percentages of 10% and 14.44%, respectively. The highest infections have occurred during winter, followed by summer, then spring seasons with isolation percentages of 53.34%, 50%, and 43.4%, respectively. The majority of the isolated bacterial spp were sensitive to Erythromycin, Florfenicol, and sulfamethoxazole-Trimethoprim. Molecular screening utilizing the sequences of 16S rRNA genes has confirmed the phylogenetic relationship to S. dysgalactiae, E. faecalis, S. iniae, and S. phocae isolates with similarity percentages exceeding 99.6%. The sequences were deposited in the GenBank with accession numbers (OK033868, OK033869, OK033870, and OK033871).
Evaluation of Skin Sympathetic Nervous Activity for Classification of Intracerebral Hemorrhage and Outcome Prediction
Authors: Xing Yantao , Cheng Hongyi , Yang Chenxi , Xiao Zhijun , Yan Chang , Chen FeiFei , Li Jiayi , Zhang Yike , Cui Chang , Li Jianqing , Liu Chengyu 
Published: April 2023

Abstract
Classification and outcome prediction of intracerebral hemorrhage (ICH) is critical for improving the survival rate of patients. Early or delayed neurological deterioration is common in ICH patients, which may lead to changes in the autonomic nervous system (ANS). Therefore, we proposed a new framework for ICH classification and outcome prediction based on skin sympathetic nervous activity (SKNA) signals. A customized measurement device presented in our previous papers was used to collect data. 117 subjects (50 healthy control subjects and 67 ICH patients) were recruited for this study to obtain their five-minute ECG and SKNA signals. We extracted the signal’s time-domain, frequency-domain, and nonlinear features and analyzed their differences between healthy control subjects and ICH patients. Subsequently, we established the ICH classification and outcome evaluation model based on the eXtreme Gradient Boosting (XGBoost). In addition, HRV as an autonomic nerve assessment method was also included as a comparison method in this study. The results showed significant differences in most features of the SKNA signal between healthy control subjects and ICH patients. The ICH patients with good outcomes have a higher change rate and complexity of SKNA signal than those with bad outcomes. In addition, the accuracy of the model for ICH classification and outcome prediction based on the SKNA signal was more than 91% and 83%, respectively. The ICH classification and outcome prediction based on the SKNA signal proved to be a feasible method in this study. Furthermore, the features of change rate and complexity, such as entropy measures, can be used to characterize the difference in SKNA signals of different groups. The method can potentially provide a new tool for rapid classification and outcome prediction of ICH patients.
Exit from Mitosis in Budding Yeast: Protein Phosphatase 1 is Required Downstream from Cdk1 Inactivation
Authors: Keaton Jason M. , Workman Benjamin G. , Xie Linfeng , Paulson James R. 
Published: April 2023

Abstract
We show that inactivation of the protein kinase Cdk1/Cyclin B (Cdc28/Clb 2 in the budding yeast Saccharomyces cerevisiae) is not only necessary for cells to leave mitosis, as is well known, but also sufficient to trigger mitotic exit. Cells carrying the mutation cdc28-as1, which makes Cdc28 (Cdk1) uniquely sensitive to the ATP analog 1NM-PP1, were arrested with spindle poisons and then treated with 1NM-PP1 to inhibit Cdk1. This treatment caused the cells to exit mitosis and enter G1-phase as shown by initiation of rebudding (without cytokinesis), production of “shmoos” (when α-factor was present), stabilization of Sic1, and degradation of Clb2. This result provides a system in which to test whether particular gene products are required downstream from Cdk1 inactivation in exit from mitosis. In this system, the mutation cdc28-as1 is combined with a conditional mutation in the gene of interest. Using this approach, we demonstrate that Protein Phosphatase 1 (PPase1; Glc7 in S. cerevisiae) is required for reestablishment of G1-phase following Cdk1 inactivation. This system could be used to test whether other protein phosphatases are also needed downstream from Cdk1 inactivation, and it could be combined with phosphoproteomics to gain information about the substrates those phosphatases act on during mitotic exit.
A New Empirical Fit to Galaxy Rotation Curves
Authors: Flynn David , Cannaliato James 
Published: April 2023

Abstract
This is a study of 84 stellar velocity curves to test an empirical solution forobserved rotation curves in galaxies. We chose the SPARC data [1] gatheredfrom 175 galaxies, then selected the 84 that had the highest quality data forrotation curve velocity and HI mass. The goal was to bring the observedvelocities back to that predicted by Kepler with a single correction factor.Using the nearest and farthest stellar velocities from each galactic data setas boundary points, we superimposed a mathematical spinning disk over theobserved stars. Using Kepler’s third law, we calculated what radius andangular velocity would correct each graph to predicted values. We foundthat an angular velocity for our model could be found to correct each of thegalaxies tested. The angular velocity is represented by ω and referred to asthe EPS curve in our graphs. We are saving our description of the processrelated to the acronym for a later paper. ω values were found to fall between 1.97 and 15.35 rads/sec with a statistical mean of 7.06.
Acute myeloid leukemia cells and MSC-derived exosomes inhibiting transformation in myelodysplastic syndrome
Authors: Liu Xiaoli , Ren Fanggang , Li Shuo , Zhang Hongyu , Wang Hongwei 
Published: April 2023

Abstract
Aims To investigate the mechanism of exosomes role in the transformation of MDS to AML.Methods Exosomes in culture supernatants of MDS and AML cell lines, were extracted by ultrafiltration and identified in three ways: morphology, size and exosome surface marker proteins. Exosomes from AML cell lines were then co-cultured with MDS cell lines and their effects on the proliferation, cycle, differentiation, apoptosis and cell microenvironment of MDS cell lines were analysed by CCK-8 assay and flow cytometry. Furthermore, exosomes from MSC were extracted for further authentication.RESULTS The transmission electron microscopy, nanoparticle tracking analysis, Western blotting and flow cytometry methods all showed that ultrafiltration is a reliable method for extracting exosomes. Exosomes from AML cell lines inhibit the proliferation of MDS cell lines, block cell cycle progression and promote apoptosis and cell differentiation. It also leads to increased secretion of tumour necrosis factor-α (TNF-α) and reactive oxygen species (ROS) in MDS cell lines. In addition, MSC-derived exosomes were found to inhibit the proliferation of MDS cell lines, arrest cell cycle progression, promote apoptosis, and inhibit differentiation.CONCLUSION In this study, we explored that exosomes of AML cell line origin affect the apoptosis of MDS cell lines via the TNF-α/ROS-Caspase3 pathway, thereby influencing the conversion of MDS to AML. Additional, MSC-derived exosomes inhibited the transformation of MDS to AML.
Bile acid derivatives from gut microbiota promote GBPs-mediated activation of caspase- 4/11 by LPS through lncRNA57RIK
Authors: Gao Yunhuan , Yue Jianmei , Ha Fushuang , Wang Ya , Wang Rong , Yang Xiaorong , Zhang Junqi , Liu Xinqi , Zhang Yuan , Han Tao , Yang Rongcun 
Published: April 2023

Abstract

Background: Gut microbiota contributes to the regulation of host immune response and homeostasis. Bile acid (BA) derivatives from gut microbiota can affect the differentiation and function of the immune cells. However, it is incompletely clear for the regulation of BA metabolites in these immune cells. Here we investigated the effects the BA metabolites on the macrophages.
Results: We here find that BA metabolites can regulate sensitivity of macrophages to LPS and or Gram-negative bacteria. BA derivatives could induce lncRNA57RIKexpression through sphingosine-1-phosphate receptor 2 (S1PR2) in the macrophages of mice and humans, which play a critical role in Gram-negative bacteria mediated IL-1βmaturation and pyroptosis of macrophages. This lncRNA57RIK could bind intracellular proteases caspase-4/11 with guanylate-binding protein 1 (GBP1) in the human and mice together to cause LPS mediated activation of caspase-4/11. Murine or human lncRNA57RIK knockout (KO) macrophages did not produce response(s) to LPS or Gram-negative bacteria. LncRNA57RIK KO mice had also reduced inflammatory responses to LPS or Salmonella typhimurium (S.T) infection.
Conclusion: Taken together, gut microbiota derived BA metabolites mediated lncRNA57RIK is necessary for LPS induced caspase-4/11 activation.
The comparative effectiveness of migraine preventive drugs: a systematic review and network meta-analysis
Authors: Lampl Christian , MaassenVanDenBrink Antoinette , Amin Faisal Mohammad , Deligianni Christina I , Gil-Gouveia Raquel , Jassal Tanvir , Sanchez-del-Rio Margarita , Reuter Uwe , Uluduz Derya , Versijpt Jan , Zeraatkar Dena , Sacco Simona 
Published: April 2023

Abstract
Objective: While there are several trials that support the efficacy of various drugs for migraine prophylaxis against placebo, there is limited evidence addressing the comparative safety and efficacy of these drugs. We conducted a systematic review and network meta-analysis to facilitate comparison between drugs for migraine prophylaxis.
Methods: We searched MEDLINE, EMBASE, CENTRAL, and clinicaltrials.gov from inception to August 13, 2022, for randomized trials of pharmacological treatments for migraine prophylaxis in adults. Reviewers worked independently and in duplicate to screen references, extract data, and assess risk of bias. We performed a frequentist network meta-analysis and assessed the certainty (quality) of evidence using the GRADE approach.
Results: We identified 74 eligible trials, reporting on 32,990 patients. We found high certainty evidence that monoclonal antibodies acting on the calcitonin gene related peptide or its receptor (CGRP(r)mAbs), gepants, and topiramate increase the proportion of patients who experience a 50% or more reduction in monthly migraine days, compared to placebo. We found moderate certainty evidence that beta-blockers, valproate, and amitriptyline increase the proportion of patients who experience a 50% or more reduction in monthly migraine days, and low certainty evidence that gabapentin may not be different from placebo. We found high certainty evidence that, compared to placebo, valproate and amitriptyline lead to substantial adverse events leading to discontinuation, moderate certainty evidence that topiramate, beta-blockers, and gabapentin increase adverse events leading to discontinuation, and moderate to high certainty evidence that (CGRP(r)mAbs) and gepants do not increase adverse events.
Conclusions: (CGRP(r)mAbs) have numerically the best safety over efficacy profile of all drugs for migraine prophylaxis, followed closely by gepants.
Multi-level flux optimization at the key regulatory node for enhanced production of naringenin using acetate in engineered Escherichia coli
Authors: Kim Dong Hwan , Hwang Hyun Gyu , Jung Gyoo Yeol 
Published: April 2023

Abstract
Background
Microbial production of naringenin has received much attention due to its pharmaceutical applicability and potential as a key molecular scaffold for various flavonoids. In particular, the oxaloacetate (OAA) node is a key regulatory node for the naringenin biosynthesis from acetate, acting as a critical linkage that reroutes tricarboxylic acid (TCA) cycle intermediates via anaplerosis of the glyoxylate cycle to the naringenin biosynthetic pathway. In this context, to efficiently produce naringenin from acetate, it is crucial to precisely regulate the carbon flux of the OAA-PEP regulatory node through appropriate pckA expression control, as the excessive overexpression of pckAcan cause the extensive loss of OAA and metabolic imbalance. However, considering the crucial impact of pckAon naringenin biosynthesis, the conventional strategy of single-level gene expression is limited in its ability to cover the large and balanced solution space, which could result in suboptimal naringenin production.
Results
This study conducted multi-layer fine-tuning of pckA expression for the precise exploration of optimal naringenin production from acetate in the large and balanced solution space. Specifically, a combinatorial expression library was generated at both transcriptional and translational levels through promoters with different strengths and rationally designed 5′-UTR variants with discrete translation efficiency. Additionally, we identified the effect of multi-level regulation of pckA expression by validating the correlation between PCK activity and naringenin production. As a result, the flux-optimized strain demonstrated a significant increase in naringenin production, with a 49.8-fold increase (and a 73.8-fold increase in naringenin yield on acetate) compared to the unoptimized strain, producing 122.12 mg/L naringenin with 20.65 mg naringenin/g acetate, which is a comparable result against those from conventional substrates.
Conclusions
Collectively, we demonstrated the significance of multi-level expression control at the key regulatory node in the metabolic pathway, covering the large and balanced solution space for precise flux rebalancing. This study proposes a platform strain for the biosynthesis of various flavonoids that can be derived from naringenin using acetate.
A bibliometric evaluation of the publications on METTL3 mediated m6A from 2000 to 2022
Authors: Wang Yingji , Tan Xiaohua , Li Xuan’an 
Published: April 2023

Abstract
Background:Many studies have demonstrated the role of METTL3 and other proteins in the N6-adenylate methylation(m6A) of RNA. It plays a role in many diseases such as cancer and tumor. However, there is a lack of comprehensive and objective reports on m6A. Therefore, this study aims to visualize the publications to quantify and determine the current status and trends of m6A with METTL3 as the catalytic core in the past two decades.
Methods: Relevant studies published between 1 January 2000 and 24 November 2022 in English were retrieved from the Web of Science Core Collection. We used four scientometric tools (Citespace v6.1.R2, VOSviewer, Scimago Graphica and Microsoft Excel 2019) to quantify and identify the individual impact and cooperation information by analyzing annual publications, the collaboration of authors, co-citation of authors, source, journals and references, cluster and co-occurrence of keywords.
Results:A total of 1349 publications were retrieved and showed a rapid upward trend. Among the top ten journals and the top ten co-cited journals, there are many top journals and their sub journals such as NATURE, CELL, SCIENCE, which indicates the fruitful results in this field. China has the highest publications, while the United States has the most significant academic influence. Chinese researchers have a keen interest in this research field, and high-yield institutions, authors and publications account for most of the ranking. It can be seen from co-cited references and reference burst that the research on biomarker, diagnosis, RRID, YTHDC1, hepatitis B virus, osteogenic differentiation and other aspects has sprung up and continues. The research of m6A pays more attention to its significance in different diseases, such as inflammation and cancer, in addition to its mechanism and role in cells.
Conclusion:METTL3 mediated m6A has achieved fruitful results at the cellular and molecular levels. The research in this field is moving from the exploration of molecular mechanism to the refinement of inflammation, cancer and other diseases, and researchers also pay special attention to its clinical application value. As a biomarker or target, METTL3 is likely to play a role in disease early warning, prognosis prediction and treatment goals.
Nitro-functionalized Fe-MOFs for lithium-sulfur batteries
Authors: Ruan Yanli , Cai Haoyu , Feng Jinshuai , Lei Haikuo , Zheng Haitao 
Published: April 2023

AbstractEnergy storage by means of lithium-sulfur batteries holds great promise. They are inexpensive and have a high potential energy density. Unfortunately, the battery's cycling performance is greatly diminished by the shuttle effect of polysulfide. Metal-organic frameworks (MOFs) with high specific surface area, nanopore size, and plentiful porosity have been proven to help prevent polysulfide migration in recent years. In this research, partially nitro-functionalized MIL-101(Fe) has been produced by combining different proportion ligands. As an electron-withdrawing group, the nitro group can reduce the charge density of the metal sites and improve the adsorption capacity of the material to polysulfides. MIL-101-NO2-0.25 showed the performance with an initial discharge capacity of 1051.5 mAh g-1at a current density of 0.5 C and maintained at 908 mAh g-1after 250 cycles.