bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of lymphatic invasion in predicting biochemical recurrence after radical prostatectomy
Authors: Chung Dae Hyuk , Han Jang Hee , Jeong Seung-Hwan , Yuk Hyeong Dong , Jeong Chang Wook , Ku Ja Hyeon , Kwak Cheol 
Published: September 2023

ObjectiveLymphatic invasion in prostate cancer is associated with poor prognosis. However, there is no consensus regarding the clinical and prognostic value of lymphatic invasion. This study aimed to investigate the prognostic value of lymphatic invasion in biochemical recurrence (BCR) and compare the recurrence rates between patients with lymphatic invasion and lymph node metastasis.MethodsWe retrospectively analyzed 2,207 patients who underwent radical prostatectomy (RP) without pelvic lymph node dissection (PLND) and 742 patients who underwent RP with PLND for clinically localized or locally advanced prostate cancer, between 1993 and 2020, at Seoul National University Hospital. Kaplan–Meier analysis was performed to estimate BCR-free survival (BCRFS) using the log-rank test. The Cox proportional hazards model was used to identify the significant factors for BCR. Propensity score matching was performed with a 1:2 ratio to match age, initial PSA level, pathological T stage, and Gleason score to exclude confounding effects.ResultsOf the 2,207 patients who underwent RP without PLND, lymphatic invasion (L1Nx) was observed in 79 (3.5%) individuals. Among the 742 patients who underwent RP with PLND, lymph node metastases were found in 105 patients (14.2%). In patients with lymph node metastasis, lymphatic invasion was observed in 50 patients (47.6%), whereas lymphatic invasion was observed in 53 patients (8.3%) among those without lymph node metastasis. In patients who underwent RP without PLND, Kaplan–Meier analysis showed significantly poorer BCR-free survival in the L1Nx group than in the L0Nx group (p &lt; 0.001). In patients who underwent RP with PLND, the L1N0, L0N1, and L1N1 groups showed significantly worse prognoses than the L0N0 group (p &lt; 0.001). However, there was no significant difference in BCRFS between the L1N0 and lymph node metastasis groups, including the L0N1 and L1N1 groups. After propensity score matching at a 1:2 ratio, the L1Nx group showed significantly poorer outcomes in terms of BCRFS than the L0Nx group (p = 0.05). In addition, the L1N0 group showed a significantly worse prognosis than the L0N0 group after propensity score matching.ConclusionLymphatic invasion in radical prostatectomy specimens is an independent prognostic factor, which can complement lymph node status for predicting biochemical recurrence. Considering lymphatic invasion as an adverse pathological finding, similar to lymph node metastasis, adjuvant therapy could be considered in patients with lymphatic invasion.
Influence of acute fasting on pain tolerance in healthy subjects: a randomised crossover study
Authors: Edwards Sophie A. , Martin Sarah L. , Rainey Timothy , Whitaker Grace , Greenwood Darren C. , Jones Anthony , Sivan Manoj 
Published: September 2023

BackgroundAlthough chronic pain and obesity are global health crises with substantial healthcare costs, little is known about the relationship between pain perception and eating behaviours. Food consumption has been reported to provide an analgesic effect by the release of neurotransmitters modulating the pain network. However, whether short-term (acute) fasting affects pain perception remains unclear.PurposeThis study aimed to investigate the effect of acute fasting on pain perception and whether attention and mood changes drove the observed changes.Patients and methodsThe cold pressor test (CPT) was used to investigate the pain tolerance of 25 healthy participants in both non-fasting and 12-h fasting sessions. They were randomised to either session with a crossover to the other after at least 24 h, with the experimenter blinded to the sessions. The pain tolerance was measured using a Stroop task in both attentive and distracted states. The Profile of Mood States (POMS) questionnaire was used to capture the mood, and a 10-point hunger scale was used to measure hunger. Mixed-effects models were used to investigate the influence of fasting and distraction on pain perception, accounting for the repeated measures.ResultsFasting reduced CPT pain tolerance, with fasting participants twice as likely to withdraw their hands early (hazard ratio = 2.4, 95% CI: 1.3–4.5). Though men tolerated CPT pain longer than women, there was no evidence that men responded to fasting differently than women (p = 0.9). In addition, no evidence supporting that fasting affected attention or mood was found. Nonetheless, it increased hunger scores by 2.7 points on a 10-point scale (95% CI: 1.2–4.2) and decreased blood glucose concentration levels by 0.51 mmol/L (95% CI: 0.19–0.84).ConclusionAcute fasting reduces pain tolerance in the healthy participants, and this effect is independent of gender and attention or mood changes.
The development and validation of an artificial intelligence-based screening method for atrial septal defect in children's chest x-rays
Authors: Zhixin Li , Gang Luo , Zhixian Ji , Silin Pan 
Published: September 2023

PurposeFor precise diagnosis and effective management of atrial septal defects, it is of utmost significance to conduct elementary screenings on children. The primary aim of this study is to develop and authenticate an objective methodology for detecting atrial septal defects by employing deep learning (DL) on chest x-ray (CXR) examinations.MethodsThis retrospective study encompassed echocardiographs and corresponding Chest x-rays that were consistently gathered at Qingdao Women's and Children's Hospital from 2018 to 2022. Based on a collaborative diagnosis report by two cardiologists with over 10 years of experience in echocardiography, these radiographs were classified as positive or negative for atrial septal defect, and then divided into training and validation datasets. An artificial intelligence model was formulated by utilizing the training dataset and fine-tuned using the validation dataset. To evaluate the efficacy of the model, an assessment of the area under the curve, sensitivity, specificity, accuracy, positive predictive value, and negative predictive value was conducted employing the validation dataset.ResultsThis research encompassed a total of 420 images from individuals. The screening accuracy and recall rate of the model surpass 90%.ConclusionsOne of profound neural network models predicated on chest x-ray radiographs (a traditional, extensively employed, and economically viable examination) proves highly advantageous in the assessment for atrial septal defect.
Analysis of 6 pediatric nephrotic syndrome cases with complications of cerebral sinovenous thrombosis and literature review
Authors: Lu Xuan , Yan Cao , Chen Hui , Wu Xiaochuan 
Published: September 2023

BackgroundCerebral venous sinus thrombosis (CVST) is a rare but serious complication of nephrotic syndrome (NS) in children. To investigate the clinical characteristics of CVST in children with NS in order to timely diagnose this complication and reduce poor outcome.MethodsCollect and analyze clinical data and magnetic resonance venography (MRV) results of children with NS complicated with CVST.ResultsData of 6 patients with NS complicated with CVST were collected. 4 of the patients were steroid-sensitive nephrotic syndrome (SSNS) and 2 were steroid-resistant nephrotic syndrome (SRNS). The occurrence of CVST was observed within a time frame ranging from 12 days to 3 years following the diagnosis of NS. One patient had two episodes of thrombosis in three years, while the other five patients had only one episode of thrombosis. All patients had proteinuria at the time of episode of thrombosis. All patients presented with headache, and three of them had strabismus, seizures, and transient blindness, respectively. Neurological examination was negative. All patients were diagnosed with CVST by MRV within 3–16 days of the onset of headache. Two patients had TRPC6 gene mutation. All patients had resolution of neurological symptoms after anticoagulation treatment.ConclusionCVST may occur in the early stages of NS. There is currently a lack of specific diagnostic indicators to reliably identify the presence of CVST in patients with NS. Children with NS who have neurological symptoms should be promptly evaluated with imaging studies. Whether TRPC6 gene mutation is also a risk factor for CVST remains to be further studied.
Zebrafish as model system for the biological characterization of CK1 inhibitors
Authors: Meier Laura , Gahr Bernd Martin , Roth Aileen , Gihring Adrian , Kirschner Stefan , Woitaske-Proske Clemens , Baier Joana , Peifer Christian , Just Steffen , Knippschild Uwe 
Published: September 2023

Introduction: The CK1 family is involved in a variety of physiological processes by regulating different signaling pathways, including the Wnt/β-catenin, the Hedgehog and the p53 signaling pathways. Mutations or dysregulation of kinases in general and of CK1 in particular are known to promote the development of cancer, neurodegenerative diseases and inflammation. There is increasing evidence that CK1 isoform specific small molecule inhibitors, including CK1δ- and CK1ε-specific inhibitors of Wnt production (IWP)-based small molecules with structural similarity to benzimidazole compounds, have promising therapeutic potential.Methods: In this study, we investigated the suitability of the zebrafish model system for the evaluation of such CK1 inhibitors. To this end, the kinetic parameters of human CK1 isoforms were compared with those of zebrafish orthologues. Furthermore, the effects of selective CK1δ inhibition during zebrafish embryonic development were analyzed in vivo.Results: The results revealed that zebrafish CK1δA and CK1δB were inhibited as effectively as human CK1δ by compounds G2-2 with IC50 values of 345 and 270 nM for CK1δA and CK1δB versus 503 nM for human CK1δ and G2-3 exhibiting IC50 values of 514 and 561 nM for zebrafish CK1δA and B, and 562 nM for human CK1δ. Furthermore, the effects of selective CK1δ inhibition on zebrafish embryonic development in vivo revealed phenotypic abnormalities indicative of downregulation of CK1δ. Treatment of zebrafish embryos with selected inhibitors resulted in marked phenotypic changes including blood stasis, heart failure, and tail malformations.Conclusion: The results suggest that the zebrafish is a suitable in vivo assay model system for initial studies of the biological relevance of CK1δ inhibition.
Monitoring beam charge during FLASH irradiations
Authors: Vojnovic Borivoj , Tullis Iain D. C. , Newman Robert G. , Petersson Kristoffer 
Published: September 2023

In recent years, FLASH irradiation has attracted significant interest in radiation research. Studies have shown that irradiation at ultra-high dose rates (FLASH) reduces the severity of toxicities in normal tissues compared to irradiation at conventional dose rates (CONV), as currently used in clinical practice. Most pre-clinical work is currently carried out using charged particle beams and the beam charge monitor described here is relevant to such beams. Any biological effect comparisons between FLASH and CONV irradiations rely on measurement of tissue dose. While well-established approaches can be used to monitor, in real time, the dose delivered during CONV irradiations, monitoring FLASH doses is not so straightforward. Recently the use of non-intercepting beam current transformers (BCTs) has been proposed for FLASH work. Such BCTs have been used for decades in numerous accelerator installations to monitor temporal and intensity beam profiles. In order to serve as monitoring dosimeters, the BCT output current must be integrated, using electronic circuitry or using software integration following signal digitisation. While sensitive enough for FLASH irradiation, where few intense pulses deliver the requisite dose, the inherent insensitivity of BCTs and the need for a wide detection bandwidth makes them less suitable for use during CONV “reference” irradiations. The purpose of this article is to remind the FLASH community of a different mode of BCT operation: direct monitoring of charge, rather than current, achieved by loading the BCT capacitively rather than resistively. The resulting resonant operation achieves very high sensitivities, enabling straightforward monitoring of output during both CONV and FLASH regimes. Historically, such inductive charge monitors have been used for single pulse work; however, a straightforward circuit modification allows selective resonance damping when repetitive pulsing is used, as during FLASH and CONV irradiations. Practical means of achieving this are presented, as are construction and signal processing details. Finally, results are presented showing the beneficial behaviour of the BCT versus an (Advanced Markus) ionisation chamber for measurements over a dose rate range, from &lt;0.1 Gys−1 to &gt;3 kGys−1.
Melatonin interaction with abscisic acid in the regulation of abiotic stress in Solanaceae family plants
Authors: Ali Muhammad , Pan Yupeng , Liu Hanqiang , Cheng Zhihui 
Published: September 2023

Solanaceous vegetable crops are cultivated and consumed worldwide. However, they often confront diverse abiotic stresses that significantly impair their growth, yield, and overall quality. This review delves into melatonin and abscisic acid (ABA) biosynthesis and their roles in abiotic stress responses. It closely examines the intricate interplay between melatonin and ABA in managing stress within plants, revealing both collaborative and antagonistic effects and elucidating the underlying molecular mechanisms. Melatonin and ABA mutually influence each other’s synthesis, metabolism and that of other plant hormones, a key focus of this study. The study highlights melatonin’s role in aiding stress management through ABA-dependent pathways and key genes in the melatonin-ABA interaction. Specifically, melatonin downregulates ABA synthesis genes and upregulates catabolism genes, leading to reduced ABA levels. It also directly scavenges H2O2, enhancing antioxidant enzyme activities, thereby underscoring their collaborative role in mediating stress responses. Moreover, the interplay between melatonin and ABA plays an essential role in multiple physiological processes of plants, including stomatal behaviors, wax accumulation, delay leaf senescence, seed germination, and seedlings growth, among others. Recognizing these relationships in Solanaceae vegetable crops holds great importance for improving agricultural practices and crop quality. In summary, this review offers a comprehensive overview of recent studies on the melatonin and ABA interplay, serving as a valuable resource for researchers and breeders dedicated to fortifying crop resilience and productivity within challenging environments.
The prevalence and factors associated with posttraumatic growth after 3-years outbreak of COVID-19 among resident physicians in China: a cross-sectional study
Authors: Zeng Zixuan , Wang Huan , Zhou Yaxing , Lu Zhanghong , Ci Renyangcuo , Lin Yezhe , Zeng Xiaoping , Huang Lei 
Published: September 2023

IntroductionThe Coronavirus disease 2019 (COVID-19) pandemic is a global traumatic event that has profoundly struck individuals’ mental health. However, this might potentially promote positive transformation such as posttraumatic growth (PTG). Studies have indicated that the COVID-19 pandemic negatively affected the well-being of resident physicians, but little is known about PTG among this vulnerable population in China. Therefore, this study investigated the prevalence and associated factors of PTG among Chinese resident physicians after 3-years outbreak of COVID-19.MethodsAn online survey was conducted from 9 March to 20 March in 2023. PTG was assessed using the 10-item Posttraumatic Growth Inventory-Short Form (PTGI-SF). Scores ≥30 implied moderate-to-high PTG. We also collected possible associated factors for PTG, including socio-demographic and psychological variables. Data was analyzed by applying descriptive statistics, univariable and multivariable logistic regression models.ResultsIn total, 2267 Chinese resident physicians provided validated data. 38.7% of them reported moderate-to-high PTG. In the multivariable logistic regression models, age (odds ratio, OR = 1.039; 95% confidence interval, 95%CI = 1.008–1.070), female (OR = 1.383, 95%CI = 1.151–1.662), satisfied or neutral with annual income (OR = 2.078, 95%CI = 1.524–2.832; OR = 1.416, 95%CI = 1.157–1.732), sufficient support at work (OR = 1.432, 95%CI = 1.171–1.751) and resilience (OR = 1.171, 95%CI = 1.096–1.252) were significantly positively associated with moderate-to-high PTG. On the contrary, burnout (OR = 0.653, 95%CI = 0.525–0.812), depression symptoms (OR = 0.700, 95%CI = 0.552–0.889), and stress (OR = 0.757, 95%CI = 0.604–0.949) were significantly negatively associated with moderate-to-high PTG.DiscussionOverall, resident physicians in China experienced relatively high prevalence of PTG that could be associated with several psychosocial factors. Findings may provide evidence to develop interventions for resident physicians to systematically and constructively process traumatic events related to the pandemic and foster their PTG.
Development and Validation of a Questionnaire to Assess Role Conflicts Among Interpreters Working With Refugee Clients: The Role Conflicts Questionnaire
Authors: Geiling Angelika , Nohr Laura , Meyer Caroline , Böttche Maria , Knaevelsrud Christine , Stammel Nadine 
Published: September 2023

Objectives: The aim of this study was to develop and validate a questionnaire to assess interpreters’ role conflicts and the challenging aspects within the triad of practitioner, interpreter and refugee client.Methods: A questionnaire was developed based on previous literature. Its factor structure and construct validity were assessed in an online survey of 164 interpreters working with refugee clients. Psychological distress (BSI-18), work-related exhaustion (CBI), and secondary traumatic stress (ProQOL) were measured to test the questionnaire’s convergent validity.Results: Exploratory structural equation modeling for categorical variables resulted in 23 items across four subscales. The scores of all subscales had good or excellent reliability (ω = 0.81 to ω = 0.93) and correlation analyses indicated convergent validity.Conclusion: The final questionnaire (RoCo) showed four clearly interpretable subscales and may help to identify emotional distress due to role conflicts among interpreters. Future studies should validate the questionnaire in different samples.
Taxonomic Identification of the Arctic Strain Nocardioides Arcticus Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress
Authors: Cong Bailin , Zhang Hui , Li Shuang , Liu Shenghao , Lin Jing , Deng Aifang , Liu Wenqi , Yang Yan 
Published: September 2023

Microorganisms living in polar regions rely on specialized mechanisms to adapt to extreme environments. The study of their stress adaptation mechanisms is a hot topic in international microbiology research. In this study, a bacterial strain (Arc9.136) isolated from Arctic marine sediments was selected to implement polyphasic taxonomic identification based on factors such as genetic characteristics, physiological and biochemical properties, and chemical composition. The results showed that strain Arc9.136 is classified to the genus Nocardioides, for which the name Nocardioides arcticus sp. nov. is proposed. The ozone hole over the Arctic leads to increased ultraviolet (UV-B) radiation, and low temperatures lead to increased dissolved content in seawater. These extreme environmental conditions result in oxidative stress, inducing a strong response in microorganisms. Based on the functional classification of significantly differentially expressed genes under 1 mM H2O2 stress, we suspect that Arc9.136 may respond to oxidative stress through the following strategies: (1) efficient utilization of various carbon sources to improve carbohydrate transport and metabolism; (2) altering ion transport and metabolism by decreasing the uptake of divalent iron (to avoid the Fenton reaction) and increasing the utilization of trivalent iron (to maintain intracellular iron homeostasis); (3) increasing the level of cell replication, DNA repair, and defense functions, repairing DNA damage caused by H2O2; (4) and changing the composition of lipids in the cell membrane and reducing the sensitivity of lipid peroxidation. This study provides insights into the stress resistance mechanisms of microorganisms in extreme environments and highlights the potential for developing low-temperature active microbial resources.
Application of the Topsis Method in Selecting the Best Laying Chicken Feed
Authors: Nami Lanni Putri 
Published: September 2023

A person's limited ability in a particular field makes them think hard about getting a job that can fulfill their daily needs. Now many people are starting to try to switch to the world of animal husbandry. One of them is raising chickens to take their eggs. Choosing good laying hen feed is a very important main factor, especially for consumption by laying hens. Therefore, a decision support system was designed in selecting the best laying hen feed using the Techinique for Order Preference by Similarity to Ideal Solution (TOPSIS) method. The TOPSIS method is an approach that determines solutions based on the closest distance from the positive ideal solution and the furthest distance from the negative ideal solution in order to obtain the optimal alternative. The result achieved is that the system is able to show which laying hen feed has the best quality which will become laying hen feed for laying hen breeders based on predetermined criteria so that Sinar Animal Husbandry can easily determine the best laying hen feed . The results of calculations using the Topsis method showed that alternative A3, namely HI-PRO-VITE 124, had the highest value with an alternative value of 0.6568
A mediação pedagógica, o conflito escolar e o itinerário educativo como ausência e potência nas políticas públicas educacionais
Authors: Pinelli Ribeiro Cleberson , Santana Novais Gercina 
Published: September 2023

Como figuram a mediação pedagógica, o conflito escolar e o itinerário educativo na política educacional brasileira? Qual matriz epistemológica orienta a inclusão desses conceitos nos documentos, programas e leis direcionados à educação? Essas perguntas objetivam apontar o quanto os profissionais da educação são lançados a termos que pressupõem definições ancoradas em suas práticas pedagógicas e que por vezes não se sabe se os fundamentos dos conceitos em questão são resultados de uma concepção teórico-prática comum ou se são a expressão personificada, de um governo, por exemplo. Metodologicamente, foi feita uma pesquisa documental, com enfoque qualitativo. Em linhas gerais não há o registro de definições por parte daqueles que formularam os documentos analisados, exceto, no Caderno de Formação da Escola da Escolha, em que a mediação pedagógica tem sua definição e matriz epistemológica apresentadas a partir da pedagogia histórico crítica, com alusão direta a Vygotsky.