bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immune dysregulation in Glycogen Storage Disease 1b - a CyTOF approach
Authors: Gehlhaar Arne , Shouval Dror , Santiago Eduardo Gonzalez , Ling Galina , McCourt Blake , Werner Lael , Yerushalmi Baruch , Konnikova Liza 
Published: February 2023

Abstract
Glycogen Storage Disease type 1b (GSD1b) is a rare disease manifesting as hypoglycemia, recurrent infections and neutropenia, resulting from deleterious mutations in the SLC37A4 gene encoding the glucose-6-phosphate transporter. The susceptibility to infections is thought to be attributed not only to the neutrophil defect, though extensive immunophenotyping characterization is currently missing. Here we apply a systems immunology approach utilizing Cytometry by Time Of Flight (CyTOF) to map the peripheral immune landscape of 6 GSD1b patients. When compared to control subjects, those with GSD1b had a significant reduction in anti-inflammatory macrophages, CD16+ macrophages, and Natural Killer cells. Additionally, there was a preference towards a central versus an effector memory phenotype in multiple T cell populations, which may suggest that these changes stem from an inability of activated immune cell populations to undergo the appropriate switch to glycolytic metabolism in the hypoglycemic conditions associated with GSD1b. Furthermore, we identified a global reduction of CD123, CD14, CCR4, CD24 and CD11b across several populations and a multi-cluster upregulation of CXCR3, hinting at a potential role of impaired immune cell trafficking in the context of GSD1b. Taken together, our data indicates that that the immune impairment observed in GSD1b patients extends far beyond neutropenia and encompasses innate and adaptive compartments, which may provide novel insights into the pathogenesis of this disorder.
Experimental study of a coupled unit for space cooling and water desalination
Authors: Sami Missaoui , Zied Driss , Romdhane Ben Slama , Bechir Chaouachi 
Published: February 2023

Abstract

The waste heat recovery from condenser of air conditioning for seawater desalination is attractive because of its great energy and environmental value. This paper experimentally investigated a modified air conditioner with a desalination unit that operates in the space-cooling and seawater distillation. The experimental investigation was used to study the performance of the system for water distillation, the flow rate of the potable water and the amount of energy saving during the desalination process. The results confirmed that the temperature in the tank is equal to 72°C. With continuously used air conditioning, this value may become better. In these conditions, the maximum coefficient of performance (COP) of the air conditioning was around 6.11. In the integrated system, the potable water production rate was 730 ml/h. The proposed system provides the possibility to save energy which would lead to low cost of fresh water. Indeed, the GHG emissions were reduced.
Microbribery in Group Identification
Authors: Erdélyi Gábor , Yang Yongjie 
Published: February 2023

Abstract
This paper studies the complexity of two microbribery problems under the model of group identification. In these problems, we are given a subset of distinguished individuals, and the questions are whether these individuals can be made socially qualified or whether they can be made exactly the socially qualified individuals, respectively, by modifying a limited number of entries in the qualifications-profile. For consent rules, the consensus-start-respecting rule, and the liberal-start-respecting rule, we obtain many NP-hardness results and polynomial-time solvability results. We also study the problems in r-profiles where each individual qualifies exactly r individuals.
Electrochemically-Selective Electrode for Quantification of Dorzolamide in Bulk Drug Substance and Dosage Form
Authors: Mora Mai M. , Ismail Nahla S. , Zaazaa Hala E. , Boltia Shereen A. 
Published: February 2023

Abstract
Three smart carbon paste electrodes were fabricated to quantify the dorzolamide hydrochloride DRZ, including conventional carbon paste I, modified carbon paste embedding Silica II, and modified carbon paste embedding β-cyclodextrin III. This study is built on the inserting of DRZ with phosphomolybdic acid to create an electroactive moiety dorzolamide-phosphomolybdate ion exchanger using a solvent mediator dibutylphthalate. The three constructed carbon paste electrodes displayed Nernstian responses and linear concentration ranges with lower detection limits. The vital performance of the created electrodes was verified with in relation to various parameters. The electrodes enhance the selective determination of DRZ in presence of inorganic ions, co-formulated drug in the dosage form timolol maleate and the excipient benzalkonium chloride. The modified carbon paste electrode inclusion Silica was utilized to detect the DRZ in ophthalmic eye drop form utilizing the direct calibration curve and potentiometric titration methods. Satisfactory findings were achieved by comparing to other reported methods.
Adipose delivered stem cells protect liver after ischemia-reperfusion injury by controlling autophagy
Authors: Kartal Bahar , Alimoğulları Ebru , Demir Hazal , Elçi Pınar 
Published: February 2023

Abstract
Background: Ischemia-reperfusion (I/R) injury is an unavoidable side effect of liver surgery and transplantation. A potentially useful tool for cellular therapy and tissue engineering is adipose-derived stem cells (ADSCs). The study's goal was to examine the impact of ADSCs in rat hepatic ischemia-reperfusion injury.
Material and methods: 30 male rats were randomly divided into the control, ADSCs, ischemia , I/R , I/R+ADSC groups (n = 6). A model for hepatic I/R injury that is evaluated by histological changes with Suzuki scores. The immunoexpression of LC3B, p62 and transforming growth factor (TGF- β)were analyzed.
Results: The ischemia and I/R groups displayed clear liver sinusoid congestion, vacuolization, and necrosis. The expression of key autophagy indicators LC3B increased whereas p62 decreased following ischemia reperfusion. TGF- β was significantly elevated in the rat liver from ischemia and I/R groups. The IRI-induced histopathological damage was improved by ADSC transplantation.
Conclusion: ADSCs reduced the excessive level of the autophagy and structural damage to hepatocytes and the pathological alterations in the liver after ıschemia-reperfusion injury.
Machine-learning based strategy identifies a robust protein biomarker panel for Alzheimer’s disease in cerebrospinal fluid
Authors: Hou Xiaosen , Quan Zhenzhen , Aierken Ailikemu , Zhao Dongxu , Ji Simei , Ni Junjun , Liu Kefu , Qing Hong 
Published: February 2023

Abstract
Background
Due to multiple pathogenesis of Alzheimer’s disease (AD), currently discovered biomarkers are stilled limited for its classification and diagnosis, robust and universal biomarkers or biomarker combinations need further to be explored.
Methods
Based on machine learning, The SVM-RFECV algorithm screened out a 12-protein panel that was applied to 5 different cohorts of AD cerebrospinal fluid (CSF) proteomic datasets.
Results
The 12-protein panel exhibited strong diagnosibility and high accuracy. It was involved in several AD related biological process and highly correlated with classical AD pathogenic biomarkers (Aβ, tau/p-tau and Montreal Cognitive Assessment (MoCA) score). It was also capable of distinguishing early stage of AD (mild cognitive impairment, MCI) as well as from other neurodegenerative diseases.
Conclusions
The SVM-RFECV algorithm has great advantages of robust predicting ability, high accuracy and good reliability for identifying AD, thus providing clues for AD pathogenesis and shedding light on AD diagnosis clinically.
Design and implementation of a 3D digestive teaching system based on virtual reality technology in modern medical education
Authors: Li Dan , Hu Weina , Zhou Xinyu , Li Li , Wang Jie , Zou Mengqing , Li Xinyi , Wang Min , Zhou Donghui 
Published: February 2023

Abstract
At present, virtual reality technology is still in its infancy in the medical field in China, and the application ratio of virtual reality technology in medical education and medical treatment is relatively low. Based on the structure of the human stomach, this study integrates virtual reality technology, sensing technology, big data technology, and cloud computing technology to form a new form of medical education with an online website platform for data storage and analysis and offline VR glasses for physical operation. The project has established a model of the morphological structure and physiological processes of the human stomach in basic medicine, a model of the pathological tissue structure and pathophysiological processes in basic medicine, a model of the pharmacological processes and clinical pharmacology in basic medicine, and a model of the integrated treatment of the human stomach in clinical medicine for medical students at the university, as well as a model of the integrated treatment of preoperative discussions in clinical medicine and a model of the integrated treatment of doctor-patient communication in clinical medicine for clinicians at the hospital. It also established a model of integrated clinical medicine preoperative discussion and treatment, a model of integrated clinical medicine doctor-patient communication, and integrated clinical medicine general health treatment. The model has effectively improved the quality and efficiency of medical education, updated the concept of medical education, facilitated the formulation of surgical plans and the reduction of surgical risks as well as provided experimental materials for scientific research, and can be further developed into a three-dimensional structure of the human circulatory system and the major systems of the human body in the future.
Removal of chromium ions by a bionanocomposite hydrogel based on starch-g-poly(acrylic acid) reinforced by cellulose nanofibers through a fix-bed adsorption column
Authors: Heidarzadeh-Samani Maryam , Behzad Tayebeh , Mehrabani-Zeinabad Arjomand , Baghbadorani Nooshin Bahadoran 
Published: February 2023

Abstract
A cellulose nanofibers reinforced starch-graft-poly(acrylic acid) (St-g-P(AA)) nanocomposite hydrogel was developed as a bio adsorbent to remove chromium ions [Cr(VI)] using a fix-bed adsorption column (FBC). The influence of several factors on adsorption column efficiency was investigated in this study, including pH, starting concentration of Cr(VI) ions, and solution input flow rate. Following the first study, the appropriate pH, starting concentration, and flow rate ranges were determined to be 4-6, 20-60 mg/L, and 5-15 mL/min, respectively. The surface response approach based on the Box-Behnken model was used to statistically examine the influence of each independent parameter on response performance (removal efficiency) and anticipate the optimal result. The findings showed that the pH of the solution had the most significant impact. The maximum removal efficiency of the nanocomposite hydrogel was 50.26 percent at pH = 6, an initial concentration of 20 mg/L, and a flow velocity of 10 mL/min. The Langmuir isotherm model successfully matched the results, and the maximum adsorption capacity was calculated to be 23.47 mg/g.
Saorilao-4 decoction alters the miRNA regulatory network in pulmonary fibrosis
Authors: Fu Xinyue , Song Xinni , Liu Yujian , Shi Songli , Chang Hong , Qi Jun , Wang Peng , Bai Wanfu 
Published: February 2023

Abstract
Objective: To explore the effect of Mongolian medicine Saorilao-4 (SRL-4) decoction on the conserved and specific miRNAs and target genes of the gene regulatory network (GRN) involved in pulmonary fibrosis.
Method: Healthy adult male SPF SD rats were randomly allocated into 4 groups. Total RNA was isolated and extracted from the lung tissues for transcriptome sequencing. The differentially expressed miRNAs (DEMs) between the different groups were screened using the difference analysis software DESeq2, and the prediction of their target genes were carried out using miRanda. Gene Ontology (GO) as well as Kyoto Encyclopedia of genes and genomes (KEGG) analyses were conducted to identify the biological functions of the target genes.
Results: There were 16 DEMs in the IPF model group, whereas SRL-4 treatment resulted in 10 DEMs relative to the untreated model group. GO analysis showed that the target genes of DEMs inSRL-4 groups were enriched in 52 GO items. In addition, KEGG analysis showed that these target genes were enriched in 182 signaling pathways, and the largest number of genes were related to purine metabolism pathway.
Conclusion: The progression of pulmonary fibrosis and SRL-4 anti-pulmonary fibrosis are significantly impacted by miR-433-3p, novel 202, and miR-150-3p.
Effects of orthodontic extraction on facial soft and hard tissue changes among average-angle skeletal class Ⅰ Chinese adults females:A 3D geometric morphometric study
Authors: Wen Zhaoxiang , Tang Yiqi , Wang Tingyu , Su Sihui , Chen Dongru , Wu Liping 
Published: February 2023

Abstract
Background: This study aimed to investigate changes in the facial soft and hard tissues changes among the average-angle skeletal class Ⅰ Chinese adult females receiving extraction orthodontic treatment, as well as the correlations between them.
Methods:
The 3dMD® stereo photography technology was used to explore facial soft tissue changes, and lateral cephalograms and cone beam computed tomography (CBCT) to explore hard tissue changes. A total of 23 adult females were recruited, among which 11 patients were with teeth extraction, 12 patients without teeth extraction treatment.
Results:
Among the 3D distance measurements, decreased soft tissue changes of 1/3 Face height, Stomatological height, Chin height and Lip width were observed (p<0.005), in which Lip width was found to have a moderate correlation with the decreased hard tissue change of upper inter-first molar width (d5) (ra=0.619, p<0.005). Among the 3D angle measurements, decreased soft tissue changes of Chin-lips angle and Lip angle, were found, in which Lip angle was found to have a moderate correlation with the decreased hard tissue changes of ∠U1-SN (ra=0.659, p<0.005). While the Soft tissue angle and Nasolabial angle increased post-orthodontics (p<0.005). In addition, moderate negative correlations between Nasolabial angle and hard tissue changes of ∠U1-SN and d1 were found (ra=-0.668; -0.763, p<0.005).
Conclusion:
Extraction orthodontic treatment showed a good control for facial protrusion cases, making the patients profile more attractive and harmonious. Changes in position and inclination of anterior teeth were also related to changes of facial soft tissues. There is no significant collapse in buccal and temporal region of patients with teeth extraction treatment compared with those without teeth extraction.
Thermomagnetic properties and the effects of Aharonov-Bohm (AB) flux and external magnetic field on Fisher entropy with Schioberg plus Manning-Rosen potential (SPMRP) using Nikiforov-Uvarov functional analysis (NUFA) and Supersymmetric quantum mechanics (SUSYQM) methods
Authors: Okon I. B. , Onate C. A. , Horchani R. , Popoola O. O. , Omugbe E. , William E. S. , Okorie U. S. , Inyang E. P. , Isonguyo C. N. , Udoh M. E. , Antia A. D. , Chen W. L. , Eyube E. S. , Araujo J. P. , Ikot A. N. 
Published: February 2023

Abstract
The analytical bound state solutions, thermomagnetic properties, and the effect of Aharanov-Bohm (AB) flux and external magnetic field on Fisher information entropy with Schioberg plus Manning-Rosen potential are studied using NUFA and SUSYQM methods in the presence of the Greene-Aldrich approximation scheme to the centrifugal term. The wave function obtained was used to study Fisher information both in position and momentum spaces for different quantum states by the gamma function and digamma polynomials. The energy equation obtained in a closed form was used to deduce numerical energy spectra, partition function, and other thermomagnetic properties. The results show that with an application of AB and magnetic fields, the numerical energy eigenvalues for different magnetic quantum spins decrease as the quantum state increases and completely removes the degeneracy of the energy spectra. Also, the numerical computation of Fisher information satisfies Fisher information inequality products, indicating that the particles are more localized in the presence of external fields than in their absence, and the trend shows complete localization of quantum mechanical particles in all quantum states. Our potential reduces to Schioberg and Manning-Rosen potentials as special cases. Our potential reduces to Schioberg and Manning-Rosen potentials as special cases. The energy equations obtained from the NUFA and SUSYQM were the same, demonstrating a high level of mathematical precision
Colorimetric assay of NO2- and mercury(II) through on-off strategy of the peroxidase-like Cu,Mo-CDs
Authors: Pu Hongmei , Cui Yifan , Wang Haidan , Yu Lijuan , Yang Dezhi , Yang Yaling , Li Hong 
Published: February 2023

Abstract
In this work, a copper-molybdenum doped carbon dots (Cu,Mo-CDs) with peroxidase-like (POD) activity was synthesized by using one-step microwave digestion method. The Cu,Mo-CDs + NO2− system can promote 3,3′,5,5′-tetramethylbenzidine (TMB) oxidize to become its blue oxidative product(oxTMB) due to the formation of ∙NO2 free radical. While Hg2+ was introduced into the system, the POD-like activity of Cu,Mo-CDs + NO2− system was inhibited by Hg2+ due to strong interaction and the electron transfer between Cu,Mo-CDs and Hg2+. The bioenzyme-free colorimetric assay based on Cu,Mo-CDs nanozyme is used for detection of NO2− and Hg2+, showing lower detection limit (LOD) of 1.63 µg/L for NO2−, 0.13 µg/L for Hg2+, respectively and good recovery from 90.79 to 108.90%. The work paves a new way to design a nanozyme-based colorimetric protocol for traces NO2− and Hg2+ residues in food analysis.
A computational fluid dynamics study for simulation of the unsteady state two-phase flow in vertical separators and plunger pumps simultaneously
Authors: Dastyar Zahra , Hajidavalloo Ebrahim , Rabieh Milad M. 
Published: February 2023

Abstract

This study aims to find a robust and reliable method to simulate unsteady state two-phase flow in the sophisticated vertical separators connected to plunger pumps using computational fluid dynamics. The unsteady nature of reciprocating pumps and vertical two-phase gas-liquid flow forces the unsteady state boundary condition to the separators. Consequently, to simulate them, the plunger pump was considered a part of geometry, and an unsteady state turbulent two-phase flow was selected for the simulation method. Due to the geometry vastness and complexity of such a problem, the mesh generation method became vital for the convergence and reliability of simulations. Among several mesh generation methods and software, a new MATLAB meshing method developed to generate structural hexahedral mesh proved to be robust and very time-efficient. First, a set of experimental data of a simple separator was used to select, tune and validate the simulation method. Then, a real sample sophisticated separator and a plunger pump were analyzed using the method. Results demonstrated the ability of the model to predict phase separation and motions and its capability for modeling two-phase flow inside the helical, gravitational, and hybrid separators together with a plunger pump. Performing simulation for three up and two down strokes of the plunger showed that after disappearing the initial condition effects, the model represents the same results and separation efficiency for all cycles of plunger movements.
Plasma based biomarkers detect radiation induced neurotoxicity in cancer patients treated for brain metastasis: A Pilot study.
Authors: Zick Aviad , Makranz Chen , Lubotzky Asael , Zemmour Hai , Shemer Ruth , Glaser Benjamin , Cohen Jonathan , Maoz Myriam , Sapir Eli , Wygoda Marc , Peretz Tamar , Weizman Noam , Feldman Jon , Abrams Ross A. , Lossos Alexander , Dor Yuval 
Published: February 2023

Abstract
Radiotherapy has an important role in the treatment of brain metastases (BrM) but carries risk of short and/or long-term toxicity, termed radiation-induced neurotoxicity (RIN). As the diagnosis of RIN is crucial for correct pa-tient management, there is an unmet need for reliable biomarkers for RIN. The aim of this proof-of concept study was to determine the utility of brain-derived circulating free DNA (BncfDNA), identified by specific methylation patterns for neurons, astrocytes, and oligodendrocytes, as RIN biomarkers. Twenty-four patients with BrM were monitored clinically and radiologically before, during and after brain radiotherapy, and blood for BncfDNA analysis (98 samples) was concurrently collected. Sixteen patients were treated with whole brain radiotherapy and eight patients with stereotactic radiosurgery. During follow-up nine RIN events were detected, and all correlated with significant increase in BncfDNA levels compared to baseline. Additionally, resolution of RIN correlated with de-crease in BncfDNA. Changes in BncfDNA were independent of tumor response. Elevated BncfDNA levels reflects brain cell injury incurred by radiotherapy, further research is needed to establish BncfDNA as a novel plasma-based biomarker for RIN.
Adult mortality patterns in Yemen before and during armed conflict and the COVID-19 pandemic: Evidence from a web survey of the global diaspora
Authors: McGowan Catherine , Alhaffar Mervat , Ekoriko Promise , Al-Refai Sawsan , Badr Jamal , Bell Lucy , Checci Francesco 
Published: February 2023

Abstract
Background
The ongoing war in Yemen has created a severe and protracted crisis that has left nearly three-quarters of the population in need of urgent humanitarian assistance. Despite eight years of conflict there exist few robust estimates of how the crisis and COVID-19 pandemic have affected mortality in Yemen. The security situation has limited access to affected populations and thus required novel alternatives to local mortality surveys.
Methods
We used a web-based, respondent-driven sampling method to disseminate a mortality survey amongst the global Yemeni diaspora. We used mortality estimation methods and survival analysis to calculate mortality and/or survivorship amongst respondents’ close family members in Yemen including adults aged 50+, siblings, and children under five years.
Results
Eighty-nine eligible respondents completed the survey. Respondents provided data on the status of 1704 individuals of whom 85 (5%) had died; of these 65 (3.8%) were reported to have died in Yemen. An analysis of survivorship of respondents’ parents after their 50th birthday (adjusted for birth cohort and gender) provided weak evidence that the war and pandemic periods were associated with higher mortality when compared to the pre-war period. Analysis of the subset of individuals who died in Yemen also suggested an increased hazard of dying during the war/pandemic period; however, these results were non-significant. Sibling mortality amongst those aged 15–49 was 0.7 per 1000 person-years during the pre-war period compared to 1.1 during the war/pandemic period amongst males, and 0.8 versus 0.0 amongst females; however, these estimates reflected small numbers of deaths. The number of deaths amongst children under five in Yemen was too low to allow meaningful analysis; only three of the seven deaths in this group occurred during the analysis period.
Conclusions
Our data suggest increased mortality during the war/pandemic period, compared to the pre-war period, among elderly Yemenis. Our findings require careful interpretation as our small and non-representative sample appeared skewed towards higher-income, urban communities. Surveys of diaspora populations offer a promising means of describing mortality patterns in crisis-affected populations; however, large numbers of respondents are likely required to achieve accurate mortality estimates and attempt adjustment for selection bias.