bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microstructure and wear resistance of laser cladding nano TiC/micro TiB2/C-276 composite coatings on 316L stainless steel
Authors: Liu He , Wang Xi , Yu Yuzhen , Yang Weiguang , Gao Hanpeng 
Published: March 2023

Abstract
Four metal-ceramic composite coatings were prepared by laser cladding (LC) using Hastelloy C-276, nano-TiC and micro-TiB2 as raw materials to improve the surface properties and extend the service life of 316L stainless steel. The microstructure and mechanical properties were analyzed by metallographic microscopy, scanning electron microscopy (SEM), x-ray diffraction (XRD), Vickers hardness experiments, and friction and wear testing. The TiC and TiB2 phases were detected in the coating, exhibiting homogenous distribution as their content increased. As a result, the strengthening effect of the coating was enhanced, improving microhardness relative to that of the 316L substrate. The wear test revealed that the Hastelloy coating exhibited poor wear resistance, but the addition of TiC and TiB2 particles improved the wear resistance by reducing the wear rate. The lowest average coefficient and wear rate were attained at 50% and 70% ceramic contents, respectively. However, abundant hard particles on the surface easily fell off during the wear, which further impeded the surface degradation and increased the friction coefficient.
Linguistic dissociation: A general theory to explain the phenomenon of linguistic distancing behaviours
Authors: Moore Ashley R 
Published: March 2023

Abstract
Across diverse yet largely unconnected reports, including language-focused research studies, psychotherapeutic case studies, literary biographies, and journalism, evidence exists of people distancing themselves from previously acquired linguistic resources, such as accents, dialects, and even named languages. In this article, I begin by discussing a selection of those reports, before arguing that there is a general process shared by these varied cases: linguistic dissociation. I then unpack my definition of linguistic dissociation, a relatively enduring psychosocial process in which an individual or group distances themselves from a set of linguistic practices already within their repertoire because those practices have come to connote a state of significant intersubjective disharmony, or contrasubjectivity. I construct a theoretical framework supported by related concepts—affective valence, evaluative conditioning, contrasubjectivity, prosubjectivity, and undesire—that provide a common theoretical vocabulary for discussing the phenomenon of linguistic dissociation and act as sensitizing tools for identifying and understanding heretofore unexamined linguistic distancing behaviours in other individuals and groups. I conclude by posing questions that future work on linguistic dissociation might answer.
GPMeta: a GPU-accelerated method for ultrarapid pathogen identification from metagenomic sequences
Authors: Wang Xuebin , Wang Taifu , Xie Zhihao , Zhang Youjin , Xia Shiqiang , Sun Ruixue , He Xinqiu , Xiang Ruizhi , Zheng Qiwen , Liu Zhencheng , Wang Jin’An , Wu Honglong , Jin Xiangqian , Chen Weijun , Li Dongfang , He Zengquan 
Published: March 2023

AbstractMetagenomic sequencing (mNGS) is a powerful diagnostic tool to detect causative pathogens in clinical microbiological testing owing to its unbiasedness and substantially reduced costs. Rapid and accurate classification of metagenomic sequences is a critical procedure for pathogen identification in dry-lab step of mNGS test. However, clinical practices of the testing technology are hampered by the challenge of classifying sequences within a clinically relevant timeframe. Here, we present GPMeta, a novel GPU-accelerated approach to ultrarapid pathogen identification from complex mNGS data, allowing users to bypass this limitation. Using mock microbial community datasets and public real metagenomic sequencing datasets from clinical samples, we show that GPMeta has not only higher accuracy but also significantly higher speed than existing state-of-the-art tools such as Bowtie2, Bwa, Kraken2 and Centrifuge. Furthermore, GPMeta offers GPMetaC clustering algorithm, a statistical model for clustering and rescoring ambiguous alignments to improve the discrimination of highly homologous sequences from microbial genomes with average nucleotide identity &gt;95%. GPMetaC exhibits higher precision and recall rate than others. GPMeta underlines its key role in the development of the mNGS test in infectious diseases that require rapid turnaround times. Further study will discern how to best and easily integrate GPMeta into routine clinical practices. GPMeta is freely accessible to non-commercial users at https://github.com/Bgi-LUSH/GPMeta.
METTL3 promotes proliferation of goat endometrial epithelial cells by regulating CTGF in an m6A-dependent manner
Authors: Sun Ya , Zhang Xin-Cheng , Li Meng-Die , Bu Li-Ge , Wang Bo , Li Ting-Yue , Ding Nai-Zheng , Ni Hua 
Published: March 2023

Abstract
N6-methyladenosine (m6A), an epigenetic modification on RNAs, plays an important role in many physiological and pathological processes. However, the involvement of m6A in goat uterus during early pregnancy remains largely unknown. In this study, we found that the total m6A level was increasing in goat uterus as early pregnancy progressed. Methyltransferase-like 3 (METTL3) is a core catalytic subunit of the m6A methyltransferase. We thus determined the expression and regulation of METTL3 in goat uterus. METTL3 was highly expressed in the luminal and glandular epithelia from day 16 (D16) to D25 of pregnancy, and it could be up-regulated by estrogen and progesterone in goat uterus and primary endometrial epithelial cells (EECs). In EECs, knockdown or overexpression of METTL3 resulted in a significant decrease or increase of cell proliferation, respectively. METTL3 knockdown reduced the m6A level of not only total RNA but also connective tissue growth factor (CTGF) mRNA. Luciferase assay suggested that METTL3 might target the potential m6A sites in the 3’untranslated region (3’UTR) of CTGF mRNA. Moreover, METTL3 positively regulated CTGF expression, and CTGF knockdown significantly counteracted the promoting effect of METTL3 overexpression on EEC proliferation. Collectively, METTL3 is dynamically expressed in goat uterus and can affect EEC proliferation by regulating CTGF in an m6A-dependent manner. Our results will lay a foundation for further studying the crucial mechanism of METTL3-mediated m6A modification in goat uterus during early pregnancy.
Clinical features and prognostic factors in adults with viral meningitis
Authors: Petersen Pelle Trier , Bodilsen Jacob , Jepsen Micha Phill Grønholm , Larsen Lykke , Storgaard Merete , Hansen Birgitte Rønde , Helweg-Larsen Jannik , Wiese Lothar , Lüttichau Hans Rudolf , Andersen Christian Østergaard , Nielsen Henrik , Brandt Christian Thomas ,   for the Danish Study Group of Infections of the Brain (DASGIB)
Published: March 2023

Abstract
Clinical features applicable to the entire spectrum of viral meningitis are limited, and prognostic factors for adverse outcomes are undetermined.
This nationwide population-based prospective cohort study included all adults with presumed and microbiologically confirmed viral meningitis in Denmark from 2015 until 2020. Prognostic factors for an unfavourable outcome (Glasgow Outcome Scale score of 1–4) 30 days after discharge were examined by modified Poisson regression.
In total, 1066 episodes of viral meningitis were included, yielding a mean annual incidence of 4.7 episodes per 100 000 persons. Pathogens were enteroviruses in 419/1066 (39%), herpes simplex virus type 2 in 171/1066 (16%), varicella-zoster virus in 162/1066 (15%), miscellaneous viruses in 31/1066 (3%) and remained unidentified in 283/1066 (27%). The median age was 33 years (IQR 27–44), and 576/1066 (54%) were females. In herpes simplex virus type 2 meningitis, 131/171 (77%) were females. Immunosuppression [32/162 (20%)] and shingles [90/149 (60%)] were frequent in varicella-zoster virus meningitis. The triad of headache, neck stiffness and hyperacusis or photophobia was present in 264/960 (28%). The median time until lumbar puncture was 3.0 h (IQR 1.3–7.1), and the median CSF leucocyte count was 160 cells/µl (IQR 60–358). The outcome was unfavourable in 216/1055 (20%) 30 days after discharge. Using unidentified pathogen as the reference, the adjusted relative risk of an unfavourable outcome was 1.34 (95% CI 0.95–1.88) for enteroviruses, 1.55 (95% CI 1.00–2.41) for herpes simplex virus type 2, 1.51 (95% CI 0.98–2.33) for varicella-zoster virus and 1.37 (95% CI 0.61–3.05) for miscellaneous viruses. The adjusted relative risk of an unfavourable outcome was 1.34 (95% CI 1.03–1.75) for females. Timing of acyclovir or valacyclovir was not associated with the outcome in meningitis caused by herpes simplex virus type 2 or varicella-zoster virus.
In summary, the outcome of viral meningitis was similar among patients with different aetiologies, including those with presumed viral meningitis but without an identified pathogen. Females had an increased risk of an unfavourable outcome. Early antiviral treatment was not associated with an improved outcome in meningitis caused by herpes simplex virus type 2 or varicella-zoster virus.
Current understanding of the chronic stress response to burn injury from human studies
Authors: Osborne Tyler , Wall Bradley , Edgar Dale W , Fairchild Timothy , Wood Fiona 
Published: March 2023

AbstractThere is a marked inflammatory and hypermetabolic response following a burn injury. The interlinked responses are more pronounced than for other forms of trauma and can persist for ≥3 years post-injury in burned patients. After a burn, patients have an increased risk of diseases of ageing including cancer, diabetes and cardiovascular disease, highlighting the need for effective long-term strategies to ameliorate the stress response post-burn. Current therapeutic strategies for post-burn recovery include removal of damaged tissue with surgical excision and wound repair, nutritional supplementation and rehabilitative exercise. These strategies aim to minimize the hypermetabolic and inflammatory responses, as well as reducing the loss of lean body mass. This review briefly summarises the inflammatory and hypermetabolic responses and provides an update on the current therapeutic strategies for burned patients. The review examines the persistent nutritional challenge of ensuring sufficient energy intake of each macronutrient to fuel the hypermetabolic and counteract the catabolic response of burn injury, whilst reducing periods of hyperglycaemia and hypertriglyceridemia. Patients require individualized treatment options tailored to unique systemic responses following a burn, facilitated by a precision medicine approach to improve clinical and physiological outcomes in burned patients. Thus, this review discusses the utility of metabolic flexibility assessment to aid clinical decision making and prescription relating to nutritional supplementation and rehabilitative exercise in the burned patient.
Early motor skill acquisition in healthy older adults: brain correlates of the learning process
Authors: Durand-Ruel Manon , Park Chang-hyun , Moyne Maëva , Maceira-Elvira Pablo , Morishita Takuya , Hummel Friedhelm C 
Published: March 2023

Abstract
Motor skill learning is a crucial process at all ages. However, healthy aging is often accompanied by a reduction in motor learning capabilities. This study characterized the brain dynamics of healthy older adults during motor skill acquisition and identified brain regions associated with changes in different components of performance. Forty-three subjects participated in a functional magnetic resonance imaging study during which they learned a sequential grip force modulation task. We evaluated the continuous changes in brain activation during practice as well as the continuous performance-related changes in brain activation. Practice of the motor skill was accompanied by increased activation in secondary motor and associative areas. In contrast, visual and frontal areas were less recruited as task execution progressed. Subjects showed significant improvements on the motor skill. While faster execution relied on parietal areas and was inversely associated with frontal activation, accuracy was related to activation in primary and secondary motor areas. Better performance was achieved by the contribution of parietal regions responsible for efficient visuomotor processing and cortical motor regions involved in the correct action selection. The results add to the understanding of online motor learning in healthy older adults, showing complementary roles of specific networks for implementing changes in precision and speed.
The cortical hypogyrification pattern in antipsychotic-naive first-episode schizophrenia
Authors: Gao Xin , Yao Li , Li Fei , Yang Chengmin , Zhu Fei , Gong Qiyong , Lui Su 
Published: March 2023

Abstract
Schizophrenia is thought to be a neurodevelopmental disease with high genetic heritability, and evidence from neuroimaging studies has consistently shown widespread cortical local gyrification index (LGI) alterations; however, genes accounting for LGI alterations in schizophrenia remain unknown. The present study examined the LGI alterations in first-episode antipsychotic-naive schizophrenia compared with controls (235 patients and 214 controls); transcription-neuroimaging association analysis was used to evaluate the relationship between LGI deficits and specific risk genes. The expression profiles of 232 schizophrenia risk genes were extracted from six donated normal brains from the Allen Human Brain Atlas database. The correlation between LGI alterations and clinical symptoms was also tested. We found lower LGI values involved in frontotemporal regions and limbic systems. Nonparametric correlation analysis showed that 83 risk genes correlated with the hypogyrification pattern in schizophrenia. These identified risk genes were functionally enriched for the development of the central nervous system. The LGI in the left superior temporal gyrus was negatively associated with Positive and Negative Syndrome Scale negative symptoms. In summary, the present study provides a set of risk genes possibly related to the hypogyrification pattern in antipsychotic-naive first-episode schizophrenia, which could help to unveil the neurobiological underpinnings of cortical impairments in early-stage schizophrenia.
Opioid Injection-Associated Bacterial Infections in England, 2002–2021: A Time Series Analysis of Seasonal Variation and the Impact of Coronavirus Disease 2019
Authors: Lewer Dan , Brothers Thomas D , Croxford Sara , Desai Monica , Emanuel Eva , Harris Magdalena , Hope Vivian D 
Published: March 2023

Abstract

Background
Bacterial infections cause substantial pain and disability among people who inject drugs. We described time trends in hospital admissions for injecting-related infections in England.

Methods
We analyzed hospital admissions in England between January 2002 and December 2021. We included patients with infections commonly caused by drug injection, including cutaneous abscesses, cellulitis, endocarditis, or osteomyelitis, and a diagnosis of opioid use disorder. We used Poisson regression to estimate seasonal variation and changes associated with coronavirus disease 2019 (COVID-19) response.

Results
There were 92 303 hospital admissions for injection-associated infections between 2002 and 2021. Eighty-seven percent were skin, soft-tissue, or vascular infections; 72% of patients were male; and the median age increased from 31 years in 2002 to 42 years in 2021. The rate of admissions reduced from 13.97 per day (95% confidence interval [CI], 13.59–14.36) in 2003 to 8.94 (95% CI, 8.64–9.25) in 2011, then increased to 18.91 (95% CI, 18.46–19.36) in 2019. At the introduction of COVID-19 response in March 2020, the rate of injection-associated infections reduced by 35.3% (95% CI, 32.1–38.4). Injection-associated infections were also seasonal; the rate was 1.21 (95% CI, 1.18–1.24) times higher in July than in February.

Conclusions
This incidence of opioid injection-associated infections varies within years and reduced following COVID-19 response measures. This suggests that social and structural factors such as housing and the degree of social mixing may contribute to the risk of infection, supporting investment in improved social conditions for this population as a means to reduce the burden of injecting-related infections.
Seasonal, environmental and individual effects on hypoxia tolerance of eastern sand darter (Ammocrypta pellucida)
Authors: Firth Britney L , Craig Paul M , Drake D Andrew R , Power Michael 
Published: March 2023

AbstractMetabolic rate and hypoxia tolerance are highly variable among individual fish in a stable environment. Understanding the variability of these measures in wild fish populations is critical for assessing adaptive potential and determining local extinction risks as a result of climate-induced fluctuations in temperature and hypoxic conditions. We assessed the field metabolic rate (FMR) and two hypoxia tolerance metrics, oxygen pressure at loss of equilibrium (PO2 at LOE) and critical oxygen tolerance (Pcrit) of wild-captured eastern sand darter (Ammocrypta pellucida), a threatened species in Canada, using field trials (June to October) that encompassed ambient water temperatures and oxygen conditions typically experienced by the species. Temperature was significantly and positively related to hypoxia tolerance but not FMR. Temperature alone explained 1%, 31% and 7% of the variability observed in FMR, LOE, and Pcrit, respectively. Environmental and fish-specific factors such as reproductive season and condition explained much of the residual variation. Reproductive season significantly affected FMR by increasing it by 159–176% over the tested temperature range. Further understanding the impact of reproductive season on metabolic rate over a temperature range is crucial for understanding how climate change could impact species fitness. Among-individual variation in FMR significantly increased with temperature while among-individual variation in both hypoxia tolerance metrics did not. A large degree of variation in FMR in the summer might allow for evolutionary rescue with increasing mean and variance of global temperatures. Findings suggest that temperature may be a weak predictor in a field setting where biotic and abiotic factors can act concurrently on variables that affect physiological tolerance.
The Silk roads: phylogeography of Central Asian dice snakes (Serpentes: Natricidae) shaped by rivers in deserts and mountain valleys
Authors: Jablonski Daniel , Mebert Konrad , Masroor Rafaqat , Simonov Evgeniy , Kukushkin Oleg , Abduraupov Timur , Hofmann Sylvia 
Published: March 2023

Abstract
Influenced by rapid changes in climate and landscape features since the Miocene, widely distributed species provide suitable models to study the environmental impact on their evolution and current genetic diversity. The dice snake Natrix tessellata, widely distributed in the Western Palearctic is one such species. We aimed to resolve a detailed phylogeography of N. tessellata with a focus on the Central Asian clade with 4 and the Anatolia clade with 3 mitochondrial lineages, trace their origin, and correlate the environmental changes that affected their distribution through time. The expected time of divergence of both clades began at 3.7 Mya in the Pliocene, reaching lineage differentiation approximately 1 million years later. The genetic diversity in both clades is rich, suggesting different ancestral areas, glacial refugia, demographic changes, and colonization routes. The Caspian lineage is the most widespread lineage in Central Asia, distributed around the Caspian Sea and reaching the foothills of the Hindu Kush Mountains in Afghanistan, and Eastern European lowlands in the west. Its distribution is limited by deserts, mountains, and cold steppe environments. Similarly, Kazakhstan and Uzbekistan lineages followed the Amu Darya and the Syr Darya water systems in Central Asia, with ranges delimited by the large Kyzylkum and Karakum deserts. On the western side, there are several lineages within the Anatolia clade that converged in the central part of the peninsula with 2 being endemic to Western Asia. The distribution of both main clades was affected by expansion from their Pleistocene glacial refugia around the Caspian Sea and in the valleys of Central Asia as well as by environmental changes, mostly through aridification.
Association of accompanying dyspnoea with diagnosis and outcome of patients presenting with acute chest discomfort
Authors: Boeddinghaus Jasper , Nestelberger Thomas , Koechlin Luca , Lopez-Ayala Pedro , Wussler Desiree , Mais Maximilian , Zwimpfer Luca , Zimmermann Tobias , Wildi Karin , Giménez Maria Rubini , Strebel Ivo , Miró Òscar , Martin-Sanchez F Javier , Parenica Jiri , Keller Dagmar I , Gualandro Danielle M , Nickel Christian H , Bingisser Roland , Christ Michael , Mueller Christian ,   for the APACE investigators
Published: March 2023

Abstract

Aims
The presence of accompanying dyspnoea is routinely assessed and common in patients presenting with acute chest pain/discomfort to the emergency department (ED). We aimed to assess the association of accompanying dyspnoea with differential diagnoses, diagnostic work-up, and outcome.

Methods and results
We enrolled patients presenting to the ED with chest pain/discomfort. Final diagnoses were adjudicated by independent cardiologists using all information including cardiac imaging. The primary diagnostic endpoint was the final diagnosis. The secondary diagnostic endpoint was the performance of high-sensitivity cardiac troponin (hs-cTn) and the European Society of Cardiology (ESC) 0/1h-algorithms for the diagnosis of myocardial infarction (MI). The prognostic endpoints were cardiovascular and all-cause mortality at two years. Among 6045 patients, 2892/6045 (48%) had accompanying dyspnoea. The prevalence of acute coronary syndrome (ACS) in patients with vs. without dyspnoea was comparable (MI 22.4% vs. 21.9%, P = 0.60, unstable angina 8.7% vs. 7.9%, P = 0.29). In contrast, patients with dyspnoea more often had cardiac, non-coronary disease (15.3% vs. 10.2%, P &lt; 0.001). Diagnostic accuracy of hs-cTnT/I concentrations was not affected by the presence of dyspnoea (area under the curve 0.89–0.91 in both groups), and the safety of the ESC 0/1h-algorithms was maintained with negative predictive values &gt;99.4%. Accompanying dyspnoea was an independent predictor for cardiovascular and all-cause death at two years [hazard ratio 1.813 (95% confidence intervals, 1.453–2.261, P &lt; 0.01)].

Conclusion
Accompanying dyspnoea was not associated with a higher prevalence of ACS but with cardiac, non-coronary disease. While the safety of the diagnostic work-up was not affected, accompanying dyspnoea was an independent predictor for cardiovascular and all-cause death.

Clinical Trial Registration
https://clinicaltrials.gov/ct2/show/NCT00470587, number NCT00470587