bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social skills in gifted students
Authors: Mendonça Lurian Dionizio , Capellini Vera Lucia Messias Fialho , Rodrigues Olga Maria Piazentin Rolim , Gastaldi Daniel German Ramos 
Published: January 2025

AbstractPeople with high skills have a different behavioural repertoire and generally, such characteristics define their type of gifted. However, we do not have conclusive data regarding the characteristics of this population, since it is an area of study that generates many controversies, and when it comes to socio‐emotional characteristics, investigations are even more scarce and conflicting. Thus, the present study tried to describe and compare the behavioural characteristics and social abilities of students with high skills, before and after participating in enrichment activities. The Strengths and Difficulties Questionnaire (SDQ) was used, with the students themselves, their parents and teachers as informants. The results showed a good repertoire of social skills and few behaviour problems in these students, but there was no significant improvement in the second evaluation. As limitations, it was pointed out: the fact that the activities of curricular enrichment do not deal with the formal teaching of social skills; the reduced size of the sample and the lack of a control group to compare the results. As strong points, it was found that there are few studies that seek to describe the socio‐emotional characteristics of this population, neglecting other needs that need to be observed and met.
De‐implementing low‐value care in emergency medicine: A framework for sustainable improvement
Authors: Yousif Fahad , Bourke Elyssia M 
Published: January 2025

AbstractThe continued use of low‐value care interventions is a persisting challenge across the healthcare system despite targeted international efforts to reduce their use. These practices result in considerable economic and carbon costs. We present a model used to successfully de‐implement four low‐value care practices in a tertiary ED in Victoria, Australia. We outline the interventions and the proportions by which they were reduced. We provide a step‐by‐step framework that provides a model for other hospital departments or primary care centres to initiate their own de‐implementation process for low‐value care practices within their setting.
Role of mitochondrial complex I genes in host plant expansion of Bactrocera tau (Tephritidae: Diptera) by CRISPR/Cas9 system
Authors: Shi Wei , He Linsheng , Li Ruixiang , Cao Jun 
Published: January 2025

AbstractHost expansion facilitates tephritid flies to expand their ranges. Unraveling the mechanisms of host expansion will help to efficiently control these pests. Our previous works showed mitochondrial complex I genes Ndufs1, Ndufs3, and Ndufa7 being upregulated during host expansion of Bactrocera tau (Walker), one of the highly hazardous species of tephritids. However, their roles in the host expansion of B. tau remain unknown. Here, using clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR‐associated nuclease 9 (Cas9) editing system for the first time, a stable homozygous Ndufa7 strain (Btndufa7−/−), heterozygous Ndufs1 (Btndufs1+/−), and Ndufs3 strains (Btndufs3+/−) were obtained from F3 generation of B. tau, after gene knockout. Reduced sizes of larvae and pupae of the Ndufa7 knockout strain were first observed. Notably, the mean values of fitness estimation (pupal numbers, single‐pupal weight and emergence rate) and Ndufa7 gene expression in the Ndufa7 knockout strain were slightly reduced on 2 native hosts (summer squash and cucumber), while it sharply decreased on the novel host banana and the potential host pitaya, compared with those of the wild‐type strain. Furthermore, the Ndufa7 knockout strain did not survive on the novel host guava. These results suggested that Ndufa7 disturbs the survival on native hosts, expansion to novel hosts, and further expansion to potential hosts of B. tau. Homozygous lethality occurred after the knockout of Ndufs1 or Ndufs3, suggesting that these 2 genes play a role in the early development of B. tau. This study revealed that Ndufa7 is a target gene for the management of tephritids and opens a new avenue for pest control research.
Fungi on the cuticle surface increase the resistance of Aedes albopictus to deltamethrin
Authors: Hu Ju‐Ping , Deng Si‐Jia , Gu Lin , Li Lin , Tu Lei , Li Ju‐Lin , Tang Jian‐Xia , Zhu Guo‐Ding 
Published: January 2025

AbstractAedes albopictus (Ae. albopictus) is widely distributed and can transmit many infectious diseases, and insecticide‐based interventions play an important role in vector control. However, increased insecticide resistance has become a severe public health problem, and the clarification of its detailed mechanism is a matter of urgence. This study found that target‐site resistance and metabolic resistance could not fully explain insecticide resistance in field Ae. albopictus, and there were likely other resistance mechanisms involved. The 16S and internal transcribed spacer sequencing revealed significant differences in the species compositions of the cuticle surface symbiotic bacteria and fungi between deltamethrin (DM)‐resistant (DR) and DM‐susceptible (DS) Ae. albopictus. Additionally, the abundances of Serratia spp. and Candida spp. significantly increased after DM treatment. Furthermore, 2 fungi (Rhodotorula mucilaginosa and Candida melibiosica) and 3 bacteria (Serratia marcescens, Klebsiella aerogenes, and Serratia sp.) isolated from DR Ae. albopictus can use DM as their sole carbon source. After reinoculation onto the cuticle surface of DS Ae. albopictus, R. mucilaginosa and C. melibiosica significantly enhanced the DM resistance of Ae. albopictus. Moreover, transcriptome sequencing of the surviving Ae. albopictus after DM exposure revealed that the gene expression of cytochrome P450 enzymes and glutathione‐S‐transferases increased, suggesting that besides the direct degradation, the candidate degrading microbes could also cause insecticide resistance via indirect enhancement of mosquito gene expression. In conclusion, we demonstrated that the cuticle surface symbiotic microbes were involved in the development of insecticide resistance in Ae. albopictus, providing novel and supplementary insights into insecticide resistance mechanisms.
Coagulation and platelet function in cold‐stored whole blood on missions in a helicopter emergency service
Authors: Sato Folatre José‐Gabriel , Wikman Agneta , Radulovic Vladimir , Sandström Göran , Skallsjö Gabriel , Arnell Per , Ricksten Sven‐Erik , Romlin Birgitta 
Published: January 2025

AbstractBackgroundHaemorrhage is a leading cause of morbidity and mortality in trauma, and prehospital transfusion of blood products is often necessary. Whole blood has been proposed to be the best alternative, but it is unclear whether, and how, storage and transport of the blood in a helicopter affects the blood units. We investigated the coagulation capacity and platelet function in whole blood at different time points during helicopter missions.MethodsTwenty units of low‐titre group O RhD negative whole blood were collected from healthy volunteers and analysed before, during and after transport in a helicopter. Coagulation and platelet function, as measured by thromboelastography, and blood samples for pH, electrolytes, glucose and lactate were assessed at baseline and 24, 72 and 168 h after storage in the helicopter. Plasma concentrations of coagulation factors and haemoglobin and blood counts were measured at baseline and after 168 h.ResultsPlasma concentrations of coagulation factors and haemoglobin did not change during storage and transport. Platelet counts decreased from a baseline mean of 172 ± 29 × 109/L to a mean of 120 ± 28 × 109/L after 168 h, and platelet function worsened slightly but significantly by 8%–9% during storage and transport. pH and glucose decreased while potassium and lactate levels increased after 168 h compared with baseline.ConclusionStorage and transport of whole‐blood units in a rescue helicopter, for up to 168 h, had a slight impact on the blood quality. Storage of whole blood on board of the helicopter holds up to European standard, measured as temperature and haemolysis.
KIF9 Ameliorates Neuropathology and Cognitive Dysfunction by Promoting Macroautophagy in a Mouse Model of Alzheimer's Disease
Authors: Wang Maoju , Guo Song , Yi Lilin , Li Zhaolun , Shi Xiuyu , Fan YePeng , Luo Man , He Yan , Song Weihong , Du Yehong , Dong Zhifang 
Published: January 2025

ABSTRACTAlzheimer's disease (AD) is a prevalent neurodegenerative disorder affecting the elderly. The imbalance of protein production and degradation processes leads to the accumulation of misfolded and abnormally aggregated amyloid‐beta (Aβ) in the extracellular space and forms senile plaques, which constitute one of the most critical pathological hallmarks of AD. KIF9, a member of the kinesin protein superfamily, mediates the anterograde transport of intracellular cargo along microtubules. However, the exact role of KIF9 in AD pathogenesis remains largely elusive. In this study, we reported that the expression of kinesin family member 9 (KIF9) in the hippocampus of APP23/PS45 double‐transgenic AD model mice declined in an age‐dependent manner, concurrent with macroautophagy dysfunction. Furthermore, we found that KIF9 mediated the transport of lysosomes through kinesin light chain 1 (KLC1), thereby participating in the degradation of amyloidogenic pathway‐related proteins of Aβ precursor protein (APP) in AD model cells through promoting the macroautophagy pathway. Importantly, genetic upregulation of KIF9 via adeno‐associated virus (AAV) diminished Aβ deposition and alleviated cognitive impairments in AD model mice by enhancing macroautophagy function. Collectively, our findings underscore the ability of KIF9 to promote macroautophagy through KLC1‐mediated anterograde transport of lysosomes, effectively ameliorating cognitive dysfunction in AD model mice. These discoveries suggest that KIF9 may represent a novel therapeutic target for the treatment of AD.
Is methamphetamine blood concentration in emergency department patients associated with the clinical picture?
Authors: McCutcheon David , Soderstrom Jessamine , Raghavan Mohan , Oosthuizen Francois , Douglas Bianca , Burrows Sally , Smith Jennifer L. , Fatovich Daniel 
Published: January 2025

AbstractAimsThis study aimed to describe clinical features and outcomes of patients presenting to the emergency department with analytically confirmed methamphetamine intoxication, to determine the blood concentration of methamphetamine and to test its association with clinical findings.DesignThe Western Australian Illicit Substance Evaluation (WISE) study is a prospective observational cohort study.SettingRoyal Perth Hospital Emergency Department, Perth, Australia, between 2016 and 2018.ParticipantsPatients suspected to be intoxicated with a stimulant, hallucinogenic or cannabinoid substance and who required intravenous access or blood tests as part of standard care. Those predominantly alcohol intoxicated, behaviourally disturbed or opioid intoxicated were excluded. The 431 participants with detectable methamphetamine (> 0.001 mg/l) included in this analysis had a mean age of 33.2 (9.4) years and 286/431 (66.4%) were male.MeasurementsConcentration was reported for methamphetamine and other illicit drugs detected. Univariate associations of symptoms and signs, and physiological and laboratory parameters with methamphetamine concentration were determined and used to develop a multivariable model.FindingsThe median concentration of methamphetamine was 0.12 mg/L [Q1,Q3: 0.05, 0.27]. Psychotic symptoms were seen in 265/431 (61.5%) patients and intravenous or intramuscular sedation was required in 280/431 (65.0%). Mean heart rate was elevated at 105.9 (21.5) beats per minute, but other mean or median physiological parameters were within normal limits. A multivariable model showed that methamphetamine concentration was 27% lower in males (P = 0.026), 60% higher in those with palpitations (P = 0.013), 62% higher in those with choreoathetoid movements (P = 0.002) and increased by 1% for each unit (μg/L) increment in creatinine (P = 0.001).ConclusionsIn a cohort of emergency department patients with methamphetamine exposure, a multivariable model inferred a significant association between higher methamphetamine concentration and female sex, the presence of palpitations and choreoathetoid movements and creatinine concentration. The model showed no significant association with agitation, psychotic symptoms or other physiological or clinical parameters.
A Newly Discovered Symbiotic Relationship Between Giant Cowbirds (Molothrus oryzivorus) and South American Tapirs (Tapirus terrestris) in Suriname
Authors: Backshall Steve J. , Dunn Christian , Mainwaring Mark C. 
Published: January 2025

ABSTRACTInterspecific interactions are a common occurrence in nature, and, in some instances, two species form very close associations. Such symbiotic relationships vary, though, in terms of the relative advantages and disadvantages accrued by both species during interspecific interactions. Symbiotic relationships whereby both species benefit are thought to occur between oxpeckers and large mammals in Africa because while oxpeckers benefit by preying upon ticks and blood‐sucking flies on the mammal's bodies, the mammals also benefit from the removal of ticks and flies. However, there is also evidence that the interactions between oxpeckers and mammals may not benefit both species because oxpeckers may not significantly reduce the abundance of ticks and may also deliberately keep wounds open, making such interactions harmful for mammals. Here, we respond to calls for further observations to help clarify the nature of such interactions by reporting a newly discovered symbiotic relationship between giant cowbirds (Molothrus oryzivorus) and South American tapirs (Tapirus terrestris) in Suriname. The cowbirds were observed feeding on ticks on the bodies of the tapirs and while it appeared that the tapirs were generally tolerant of the cowbirds, we did observe the tapirs attempting to repel the cowbirds when they appeared to target wounds on their bodies. The interactions we observed therefore appeared to benefit the cowbirds by providing them with prey and while the tapirs benefit from a reduction in tick abundance, the cowbirds did appear to target wounds on their bodies. Consequently, we tentatively conclude that the relationship between giant cowbirds and South American tapirs that we observed was beneficial to the birds and yet potentially harmful to the mammals and so further observations are needed to confirm if such interactions are context dependent. The interactions between giant cowbirds and South American tapirs provide an interesting opportunity to examine the nature of the evolutionary causes and ecological consequences of symbiotic interactions between birds and mammals.
Homozygous multiple‐insecticide resistance in Australian invasive serpentine leafminer Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae)
Authors: Chen Yizhou , Nguyen Duong T. , Webster John , Nguyen Thi Hong Tham , Herron Grant A. 
Published: January 2025

AbstractIn October 2020, Liriomyza huidobrensis (serpentine leafminer [SLM]) was first detected in Western Sydney, New South Wales (NSW), and subsequently Southern Queensland (QLD). Control failures were reported, and insecticide resistance was confirmed via bioassay, but mechanisms causing resistance remained unknown. We characterised the complete mRNA sequence of eight insecticide target genes of L. huidobrensis using RNA sequencing. We found that the Australian SLM carries three mutations (I129V, G227A and F331W) of the acetylcholinesterase (AChE) gene, causing resistance to mode of action (MOA) 1 chemicals; one mutation (A301S) of the gamma‐aminobutyric acid receptor subunit beta (GABAR), causing resistance to MOA 2 chemicals; and two mutations (M918T and L1014L) of the voltage‐gated sodium channel (VGSC) gene, causing resistance to MOA 3 chemicals. We consequently developed a multiamplicon‐sequencing panel to screen 234 Australian field‐collected SLM samples using next‐generation sequencing. The multiplex panel includes mitochondrial cytochrome oxidase subunit 1 (COI) for species identification and AChE, VGSC, glutamate‐gated chloride channel (GluCl) and chitin synthase 1 (CHS1) gene mutations. We confirm that all the individuals carry the multi‐resistance alleles in a homozygous and fixed state. This is a rare phenomenon for a single individual to be homozygous for multiple resistance mutations, with only a few studies documenting such multi‐resistance at the population level. Such resistance mechanism detection raises concern that there are limited chemical options for the control of invasive SLM in Australia.
IL‐25 Enhances B Cell Responses in Type 2 Inflammation Through IL‐17RB Receptor
Authors: Abdu Semah , Xia Jiao , Yuan Huihui , Tan Tiak Ju , Layhadi Janice A. , Shamji Mohamed H. , McKenzie Andrew N. J. , Haloob Nora , Hopkins Claire , Woszczek Grzegorz , Till Stephen J. 
Published: January 2025

ABSTRACTBackgroundAlarmin cytokine IL‐25 promotes type 2 inflammatory responses in disorders such as asthma and chronic rhinosinusitis with nasal polyps (CRSwNP) and known targets include ILC2 and Th2 cells. However, other cellular targets for IL‐25 remain poorly defined.ObjectiveTo investigate induction and expression of IL‐25 receptor (IL‐17RB) by B cells and evaluate responsiveness of IL‐17RB‐expressing B cells to IL‐25 in vitro.MethodsIL‐17RB expression, regulation and function on B cells were evaluated in peripheral blood‐derived B cells by flow cytometry and RT‐PCR, including in response to IgE‐inducing stimuli (anti‐CD40 mAb and IL‐4). Single‐cell RNA sequencing was used to compare IL‐17RB+ and IL‐17RB‐activated peripheral blood‐derived B cells. To evaluate B cell IL‐17RB expression within type 2 inflamed tissue, B cells were compared from nasal polyps, control turbinate tissue and matched peripheral blood.ResultsActivation of B cells with anti‐CD40 and IL‐4 increased IL‐17RB expression at both protein and mRNA level, which was further upregulated by IL‐25. B cells induced to express IL‐17RB responded to IL‐25 with enhanced antibody production. Single‐cell RNA‐sequencing showed that IL17RB+ activated B cells expressed higher levels of IGHE, CCL17 and CCL22 compared to IL17RB‐ B cells. B cells from nasal polyp tissue expressed higher levels of surface IL‐17RB compared with control tissue, correlating with patient‐reported CRSwNP severity (SNOT‐22).ConclusionPeripheral blood B cells activated under IgE‐inducing conditions express surface IL‐17RB, and tissue IL‐17RB+ B cells are increased in type 2 inflammation. IL‐17RB+ cells have a distinct transcriptional profile and respond to IL‐25 with enhanced antibody production, highlighting the IL‐25/IL‐17RB pathway as a potential therapeutic target for CRSwNP and other type 2 inflammatory disorders.
Macular oedema due to brvo: Timing for vitrectomy
Authors: Gkizis Ilias 
Published: January 2025

Retinal vein occlusion (RVO), either central (CRVO), branch RVO or hemiretinal RVO is the second most common retinal vascular disease after diabetic retinopathy. It may lead to irreversible visual loss if left untreated. The most common cause of decreased vision in patients affected with BRVO is the accumulation of fluid within the macula.Currently, in our clinical practice we have various treatment options for macular edema associated with BRVO. As a first line treatment the use of anti‐VEGF injections is very common and can be combined with the use of Grid‐pattern laser photocoagulation. Furthermore, in cases resistant to anti‐VEGF the intravitreal steroid implant (DEX) is an alternative choice.Epiretinal membrane is a fibroglial cellular tissue proliferation in the macula and is most commonly idiopathic, generated after a posterior vitreous detachment (PVD). Secondary ERM has been described in ocular trauma, retinal detachment, retinal surgery, retinal vasculopathies, and ocular inflammation. The prevalence of ERM in patients with RVO ranges between 14 and 16% and may exacerbate macular edema and reduce drug penetration of anti‐VEGF agents.In cases with BRVO, resistant macular edema despite of continuous anti‐VEGF treatment overtime and the presence of ERM the surgical removal of the membrane could potentially benefit patients, with a favorable final anatomical and functional result. Many parameters must be evaluated preoperatively, such as the integrity of the photoreceptor IS/OS, external limiting membrane (ELM), the absence of macular ischemia and the absence of disorganization of inner retinal layers (DRIL) before proceeding with retinal surgery.Vitrectomy with ERM peeling in these patients could lead to significant improvement in visual acuity and macular thickening, with resolution of macular edema and reduce the need for anti‐VEGF injections.
Monogenic disease associated with uveitis
Authors: Heissigerova Jarmila 
Published: January 2025

The aetiology of non‐infectious uveitis is usually multifactorial, involving both genetic and environmental risk factors. The association of human leucocyte antigens (HLA) with some types of uveitis is well recognized, such as HLA‐A29 and Birdshot chorioretinitis, or HLA‐B27 and acute anterior uveitis alone or with ankylosing spondylitis. The monogenic associations with uveitis, such as in Blau syndrome or Haploinsufficiency of A20, are extremely rare and as such due to limited experience with the disease difficult to treat or manage if the inflammation is of sight‐threatening nature. The paper will report patients from Centre for diagnosis and treatment of uveitis in Prague, and our diagnostic and therapeutic experience with monogenic diseases associated with uveitis, especially with Blau syndrome.Supported by AZV MZ CR NU23‐05‐00133.