bluuebunny/crossref_metadata_2025_split huggingface dataset:
2-deoxy-D-glucose chemical exchange-sensitive spin-lock MRI of cerebral glucose metabolism after stroke in the rat
Authors: Boehm-Sturm Philipp , Schuenke Patrick , Foddis Marco , Mueller Susanne , Koch Stefan P. , Beard Daniel J. , Holloway Paul , Mottahedin Amin , Schröder Leif , Buchan Alastair M. , Mergenthaler Philipp 
Published: January 2025

AbstractRapid breakdown of cerebral glucose metabolism is a hallmark in stroke pathology. Metabolic activity delineates the penumbra from the infarct core, representing tissue that is potentially salvageable by therapeutic interventions. Tools to image dynamics of glucose and its spatial distribution could provide biomarkers of disease severity and of the success of therapeutic interventions.Here, we developed a new protocol to measure glucose metabolism in a rat model of stroke using chemical exchange-sensitive spin-lock (CESL) MRI of the glucose analog 2-deoxy-D-glucose (2DG). We further implemented a protocol that combines 2DG-CESL-MRI with perfusion and diffusion MRI to relate this new signal to established definitions of hypoperfused tissue, cytotoxic edema and the penumbra. We found that 2DG-CESL-MRI provides a biomarker of disturbed glucose metabolism after stroke with high effect size. This is the first study to investigate CESL MRI of 2DG in the context of metabolism imaging in rodent stroke.
Genome-wide Viral Nascent RNA Sequencing Unveils Polymerase Pausing Landscape at Single-nucleotide Precision
Authors: Zhu Zhitong , Fung Cheuk Wang , Xu Xinzhou , Liang Zhibin , Gao Zhuoran , Wang Maggie Haitian , Li Mingyuan , Yeung Sin Yu , Wang Jiguang , Tse Carmen Ka Man , Cheung Peter Pak-Hang 
Published: January 2025

AbstractUnderstanding viral replication and transcription mechanisms is critical for developing effective antiviral strategies. The study of viral gene regulation in host cellular environment is an important bridge for translating mechanistic discoveries fromin vitrostudies toin vivobut it remains stagnated due to the absence of technological advancement. Current methods for studying viral transcription and replication have been limited to capturing only the mature viral RNAs, obscuring the dynamic intermediates and mechanistic details of these crucial processes. Here, we introduce an original technology we called “Total Elongating Nascent VIral Polymerase single-molecule Sequencing (TenVIP-seq)”, which isolates and analyses newly synthesized RNA within the viral RNA-dependent RNA polymerase (RdRp) complex, enabling the discovery of mechanisms critical for both viral replication and transcription. Our first characterization of nascent RNA species for an RNA virus showed that RdRP exhibited non-random pausing with profile signatures along the eight influenza A virus (IAV) gene segments. We also revealed genome-wide pausing at known regulatory sites, such as the poly(U) tract polyadenylation site, and new putative regulatory sites at single-nucleotide resolution. Distinct pausing features between the viral genomic (vRNA) and anti-genomic (cRNA/mRNA) templates were observed, suggesting that RdRp processes transcription and replication differentially on positive and negative sense RNA. The NTP analog drug T-705 (favipiravir) intensified RdRp pauses during genome replication and transcription without introducing new pausing sites, while TRIM25 knockout in host cells infected with virus reduced RdRp pausing globally across the viral genome. Strikingly, we observed that terminal nucleotide misincorporations of nascent RNA sequencing of paused RdRP, which were previously undetectable, were as high as an average of 51.6% (vRNA) and 44.0% (cRNA/mRNA), suggesting that the mutational rate of viruses is much higher than previously thought. We demonstrated that TenVIP-seq holds potential in providing insights into the molecular mechanisms that control viral genome replication, gene expression and regulation, mutation, antiviral drug treatment, and virus-host interaction.
Crime of misappropriation of assets in the business context. A systematic review
Authors: Jimenez Serrano Jorge , Delgado Rodríguez María Jesús , Rodríguez Iglesias Isabel , López Pérez Rafael Manuel 
Published: January 2025

Purpose
The purpose of this article is to deepen on the crime of assets misappropriation (AM) in the company, collecting through a systematic review of the published scientific literature those factors or theories that are being used when writing about this type of crime, as well as the implications for the business context.

Design/methodology/approach
The PRISMA methodology of systematic review has been used, from which 21 articles have been included, all of them published since 2005 in scientific journals related to the financial and economic context, but also to the criminological approach.

Findings
The articles provide several factors that are currently of interest in the study of the crime of AM in the company: the type of company in which this crime occurs, the most frequent types of AM, the elements of prevention used, as well as the profile and characteristics of employees who usually commit this type of crime.

Research limitations/implications
The conclusions of this article show the importance of studying this type of crime to combat it, not only because of the economic implications but also because of how it affects other spheres of the business context. The research has focused exclusively on articles written in English with the keyword “misappropriation assets”, which may have limited the inclusion of other articles of interest, although the objective of this study is precisely to evaluate the scientific interest in this specific type of Fraud.

Practical implications
Conclusions are drawn regarding prevention elements that are currently being used in the company to detect and combat this type of crime, as well as suggestions for future research.

Originality/value
This article seeks to support and encourage the study of this type of crime in business, following the sustainable development goals, which are the global goals designed to eradicate poverty, protect the planet and ensure global prosperity, among which is the promotion of business ethics and transparency in economic activities, for which the crime of misappropriation is a violation of these principles.
Perceiving and accessing resources in uncertain environments: insights from waste collection ventures in Burkina Faso
Authors: Dal Fior Catherine , Huybrechts Benjamin , Dufays Frédéric 
Published: January 2025

PurposeThis article explores how waste collection venture founders in an uncertain sub-Saharan African environment perceive and access resources. More particularly, it investigates why, even in a similar context, different types of resource-mobilizing practices can be observed among venture founders and how these different practices can be related to founders’ diverging perceptions of resource accessibility.Design/methodology/approachThe study compares seven waste collection ventures in Ouagadougou, Burkina Faso, a particularly uncertain market with shifting public regulation. The comparative case study analysis relies on interviews with venture founders, staff members and sector experts, as well as observations and archival data.FindingsThe findings suggest that the diverse approaches to resource accessibility can be associated with different ways in which venture founders perceive three key dimensions: environmental uncertainty (which is not necessarily seen as negative), the venture’s mission (for-profit or not-for-profit) and the founders’ self-perceptions. Three “perception-practice” patterns are identified, which illuminate different avenues for waste collection venture founders to access resources and position themselves in between local traditions and international influences.Research limitations/implicationsThe findings contribute to refining the understanding of the links between entrepreneurial perceptions and resource access in uncertain environments, and further illuminate the diversity and complexity of entrepreneurial approaches in sub-Saharan Africa.Practical implicationsThe findings of this paper may help waste management entrepreneurs better leverage resources and deal with uncertainty. Moreover, the paper includes recommendations to public authorities in charge of waste policy at the local, national and international levels, urging them to take the diversity of entrepreneurial approaches into consideration and formulate tailored policies to support waste entrepreneurs in accessing the resources they need.Social implicationsInforming the diversity of waste management practices and their effectiveness directly contributes to supporting small venture development and dealing with pollution, thereby addressing, respectively, sustainable development goals 8 (“Economic development and growth”) and 15 (“Life on land”).Originality/valueAs entrepreneurship in sub-Saharan Africa remains relatively underexplored in comparison with Western contexts, in particular from the perspective of entrepreneurial perceptions, the originality of this article is to connect resource access practices with the different perceptions unfolding in a similar context, thereby shedding light on how such diversity informs the understanding of entrepreneurial practices in uncertain contexts.
Unlocking the enigma of social commerce discontinuation: exploring the approach and avoidance drivers
Authors: Chong Sin-Er , Lim Xin-Jean , Ng Siew Imm , Kamal Basha Norazlyn 
Published: January 2025

PurposeThis research explores the impact of approach and avoidance drivers on users’ discontinuance usage intention (DUI) in social commerce (s-commerce). It also examines the mediating role of perceived enjoyment and the moderating role of autotelic personality traits.Design/methodology/approachUsing a purposive sample of 465 s-commerce users in Malaysia, data analysis was conducted using partial least squares structural equation modeling (PLS-SEM).FindingsThis study demonstrates that informational support is a key approach driver, enhancing perceived enjoyment and reducing perceived deception and DUI. Although perceived deception does not directly affect perceived enjoyment, it significantly predicts DUI. Additionally, the impact of informational support on DUI is mediated by perceived enjoyment and moderated by autotelic personality.Practical implicationsS-commerce stakeholders, including platform developers, sellers and policymakers, can leverage these findings to foster a sustainable s-commerce environment. Implementing the proposed strategies can enhance perceived enjoyment and reduce DUI among current users.Originality/valueThis research advances s-commerce marketing and IS discontinuance studies by extending the approach-avoidance motivations model (AAMM) into s-commerce and incorporating autotelic personality as a moderating factor provides new insights into user DUI in this evolving field.
Exploring the nexus between corporate social responsibility and consumer green behavior: the interplay of corporate image, service quality, customer trust and satisfaction
Authors: Liu HengYuan , Ma Sihan , AlWadi Belal Mahmoud , Alam Fahad , Zhang YueFeng 
Published: January 2025

Purpose
In an era marked by growing environmental concerns, businesses are increasingly recognizing the importance of Corporate Social Responsibility (CSR) in influencing customer behavior, particularly in the context of sustainability and green practices. This paper aims to examine the impact of Corporate Social Responsibility (CSR) on Customer Green Behavior (CGB) through the mediating role of corporate image, service quality, customer trust and customer satisfaction.

Design/methodology/approach
A convenient sampling technique was employed to collect the data sample. A total of 741 questionnaires were distributed across four different hotel sectors in China. By using Structural Equation Modeling, the results suggest that CSR significantly influences CGB. Moreover, corporate image, service quality, customer trust and customer satisfaction show a partial mediating effect in the relationship between CSR and CGB.

Findings
The study findings suggest that the hotel industry should invest in CSR initiatives to enhance CGB by conducting pro-environmental activities. This study emphasizes how important CSR initiatives are in encouraging customers to adopt eco-friendly behavior. Overall, the results of this study extend the understanding of CSR, CGB, corporate image, service quality, customer trust and customer satisfaction in the context of the hotel industry and offer theoretical and managerial implications for developing and developed economies.

Originality/value
The originality value of this research lies in its comprehensive examination of the mediating effects of corporate image, service quality, customer trust and customer satisfaction on the relationship between CSR and CGB in the hotel industry. Furthermore, the study’s focus on the specific context of China adds novel insights to the existing literature on CSR and CGB. Discussions, limitations and research suggestions for future study are also provided.
Nitrogen content of herbarium specimens from arable fields and mesic meadows reflect the intensifying agricultural management during the 20th century
Authors: Kühn Paul , Umazekabiri Raymond , Römermann Christine , Bruelheide Helge , Wesche Karsten 
Published: January 2025

Abstract

Arable fields and mesic meadows have been affected by intensifying agricultural management and nutrient input during the 20th century, but direct evidence for the long‐term impact of intensification on plant nutrient contents remains scarce. Non‐destructive novel spectroscopic methods can produce such data from herbarium specimens, making it possible to investigate how contents of leaf nutrient traits, especially nitrogen and phosphorus, changed over the last century, and what role habitat type and management practices play.

We carried out a resurvey study of functional traits in arable field and mesic meadow communities. We used specimens from two German herbaria with a high coverage of their local floras: the herbaria Senckenberg Görlitz and Senckenberg Haussknecht in Jena. Following specimen information, the same plant species were resampled in the field in 2022 at the same locations. We employed near‐infrared spectroscopy to predict leaf nitrogen, phosphorus and carbon content of herbarium and field specimens. Nutrient content changes over time were compared with public records of regional P and N fertilization.

Overall, 1270 specimens of 76 species from both herbarium and field were studied, the oldest from the 19th century. Leaf nitrogen and the leaf nitrogen:phosphorus ratio increased significantly through time, while leaf phosphorus and carbon content decreased significantly over time. Arable field species showed a stronger response in leaf phosphorus content and leaf nitrogen:phosphorus ratio than mesic meadow species. The total amount of nitrogen or phosphorus fertilizer applied per year on a regional scale was found to be significantly correlated with the respective leaf nutrient content levels.

Synthesis: Our study shows a long‐term increase of leaf nitrogen in the studied habitats, paralleling increased chemical fertilizer applications in Germany. Our data indicate a shift from predominantly N‐limited towards more P‐limited growth conditions. The stronger response of species from arable fields compared to species from mesic meadows could indicate a faster adjustment to environmental pressures. This study thus also serves to showcase the potential of the combination of herbarium collections and NIR spectroscopy.
A Novel Lineage of Endosymbiotic Actinomycetales: Genome Reduction and Acquisition of New Functions in Bifidobacteriaceae Associated With Termite Gut Flagellates
Authors: Silva Joana Kästle , Hervé Vincent , Mies Undine S. , Platt Katja , Brune Andreas 
Published: January 2025

ABSTRACTCellulolytic flagellates are essential for the symbiotic digestion of lignocellulose in the gut of lower termites. Most species are associated with host‐specific consortia of bacterial symbionts from various phyla. 16S rRNA‐based diversity studies and taxon‐specific fluorescence in situ hybridization revealed a termite‐specific clade of Actinomycetales that colonise the cytoplasm of Trichonympha spp. and other gut flagellates, representing the only known case of intracellular Actinomycetota in protists. Comparative analysis of eleven metagenome‐assembled genomes from lower termites allowed us to describe them as new genera of Bifidobacteriaceae. Like the previously investigated Candidatus Ancillula trichonymphae, they ferment sugars via the bifidobacterium shunt but, unlike their free‐living relatives, experienced significant genome erosion. Additionally, they acquired new functions by horizontal gene transfer from other gut bacteria, including the capacity to produce hydrogen. Members of the genus Ancillula (average genome size 1.56 ± 0.2 Mbp) retained most pathways for the synthesis of amino acids, including a threonine/serine exporter, providing concrete evidence for the basis of the mutualistic relationship with their host. By contrast, Opitulatrix species (1.23 ± 0.1 Mbp) lost most of their biosynthetic capacities, indicating that an originally mutualistic symbiosis is on the decline.
Insights Into Phylogeny, Diversity and Functional Potential of Poseidoniales Viruses
Authors: Prabhu Apoorva , Zaugg Julian , Chan Cheong Xin , McIlroy Simon J. , Rinke Chris 
Published: January 2025

ABSTRACTViruses infecting archaea play significant ecological roles in marine ecosystems through host infection and lysis, yet they have remained an underexplored component of the virosphere. In this study, we recovered 451 archaeal viruses from a subtropical estuary, identifying 63 that are associated with the dominant marine order Poseidoniales (Marine Group II Archaea). Phylogenetic analyses of a subset of complete and nearly‐complete viral genomes assigned these viruses to the order Magrovirales, a lineage of Poseidoniales viruses, and identified a novel group of viruses distinct from Magrovirales. Utilising demarcation criteria established for the classification of archaeal tailed viruses, we propose two families within the order Magrovirales: Apasviridae (magrovirus group A), comprising the genera Agnivirus and Savitrvirus, and Krittikaviridae (magrovirus group E) encompassing the genus Velanvirus. Additionally, we propose a new order, distinct from Magrovirales, named Adrikavirales, which includes the genus Vyasavirus. Our detailed genomic characterisation of the new viral lineages revealed genes involved in viral assembly and egress, such as those responsible for creating holin rafts to lyse host cell membranes, a feature predominantly known from bacteriophages. Furthermore, we identified a broad spectrum of auxiliary metabolic genes, suggesting that these viruses can modulate host metabolism. Collectively, our findings substantially enhance the current understanding of the diversity and functional potential of Poseidoniales viruses.
Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation
Authors: Perez María Florencia , Angelov Angel , Übelacker Maria , Torres Tejerizo Gonzalo Arturo , Farias María Eugenia , Liebl Wolfgang , Dib Julián Rafael 
Published: January 2025

ABSTRACTActinobacteria have frequently been reported in the Andean Puna, including strains of the genus Micrococcus. These strains demonstrate resistance to high levels of UV radiation, arsenic, and multiple antibiotics, and possess large linear plasmids. A comparative analysis of the sequences and putative functions of these plasmids was conducted. The presence of large regions with high sequence identity (exceeding 30 kb in total) in all three studied Micrococcus megaplasmids indicates a clear evolutionary link among these elements. Genes related to essential plasmid functions were primarily found within these conserved regions, while genes associated with resistance to metals and antibiotics resided in accessory regions. Moreover, the abundance of open reading frames related to transposition and recombination, along with local deviations from the average GC content, provides evidence for the mosaic nature and considerable genetic plasticity of these plasmids. This study presents evidence of a common ancestor for linear plasmids in Micrococcus and suggests that horizontal gene transfer likely occurs frequently within Andean lakes, providing the native microbial community with a beneficial gene pool to withstand extreme conditions. Additionally, the successful transfer of the linear plasmid pLMA1 by a DNase‐insensitive, conjugation‐type mechanism and its potential use as a genetic vector is demonstrated.
Silent Suffering: Using Machine Learning to Measure CEO Depression
Authors: CHENG SUNG‐YUAN (MARK) , GOLSHAN NARGESS M. 
Published: January 2025

ABSTRACTWe introduce a novel measure of CEO depression by applying machine learning models that analyze vocal acoustic features from CEOs' conference call recordings. Our research was preregistered via the Journal of Accounting Research's registration‐based editorial process. In this study, we validate this measure and examine associated factors. We find that greater firm risk is positively associated with CEO depression, whereas higher job demands are negatively associated with CEO depression. Female and older CEOs show a lower likelihood of depression. Using this novel measure, we then explore the relationship between CEO depression and career outcomes. Although we do not find any evidence that CEO depression is associated with CEO turnover, we find some evidence that turnover‐performance sensitivity is higher among depressed CEOs. We also find limited evidence of higher compensation and higher pay‐performance sensitivity for depressed CEOs. This study provides new insights into the relationship between CEO mental health and career outcomes.
Lymph Node Metastasis for pN+ Superficial Esophageal Squamous Cell Carcinoma
Authors: Yang Yafan , Xue Liyan , Chen Xiankai , Kang Mingqiang , Zhang Renquan , Tian Hui , Ma Jianqun , Fu Maoyong , Wei Jinchang , Liu Qi , Hao Anlin , He Yi , Zhang Ruixiang , Xie Hounai , Xu Lei , Luo Peng , Qin Jianjun , Li Yin 
Published: January 2025

ABSTRACTObjectivesThis study aimed to analyze lymph node metastasis (LNM) distribution in superficial esophageal squamous cell carcinoma (ESCC) and its impact factors on survival.MethodsWe reviewed 241 pT1N+ ESCC cases between February 2012 and April 2022 from 10 Chinese hospitals with a high volume of esophageal cancer (EC). We analyzed clinicopathological data to identify overall survival (OS) risk factors and LNM distribution in relation to tumor invasion depth.ResultsOf the 241 patients, 26 (10.8%) had pT1a cancer and 215 (89.2%) had pT1b cancer. We showed that N3 stage, ≤ 28 lymphadenectomies, and nerve infiltration (NI) were negative factors for OS in superficial pN+ ESCC, whereas the OS was not definitively affected by the tumor depth and the choice of adjuvant therapy. In general, the LNM rates of the 193 pT1N+ ESCC cases can be ranked in the following order: station 106recR > station 106recL > station 1 > station 7 > station 2. With deeper tumor invasion, the higher LNM rate was observed near the bilateral recurrent laryngeal nerves (RLN), but there was no statistically significant difference.ConclusionsIn superficial ESCC, LNM was frequently observed along the 106recR (35.8%) and 106recL (25.6%) stations. Advanced N‐staging (N3) was a major negative impact factor in prognosis, and adequate lymph nodes dissected (LND) (N > 28) improved OS of pT1N+ ESCC. However, in superficial ESCC, tumor infiltration depth did not affect patients' OS or the distribution of positive LNs. The optimal adjuvant treatment that favors survival for these patients required further investigation.