bluuebunny/crossref_metadata_2025_split huggingface dataset:
Good practices for the development of budget impact models at regional level
Authors: Marcellusi Andrea , Ragonese Angela , Marinozzi Andrea , Bortolami Alberto , Mucherino Sara , Moreno Carolina , Antenori Amalia , Ferrario Matteo , Simonelli Claudia , Zanuzzi Matteo , Cicoira Marco , Lasala Ruggero , Russoniello Francesco , Attanasio Francesco , Donati Caterina , Roni Chiara , Gemmi Fabrizio , Mennini Francesco Saverio , Russo Pierluigi , Scroccaro Giovanna , Canonico PierLuigi 
Published: June 2023

Introduction: The present work aims to discuss the current scenario of procedures and regulations regarding budget impact analysis/models (BIA/BIM) at regional level in Italy and to provide a standardized approach and detailed recommendations for developing these analyses. Method: A systematic review of the literature was conducted in order to collect existing guidelines or specific regional procedures for budget impact analysis in Italy. All the records were analysed in qualitative terms according to a pre-specified analytical framework, based on the ISPOR BIA guidelines. At the end of the analysis, a consensus questionnaire was developed to establish agreed approaches and to provide possible solutions to any critical issues. A list of 39 statements was developed. The survey was distributed to 69 experts who rated their level of agreement with each statement on a 5-point Likert scale. Consensus was predefined as more than 66% of the panel agreeing/disagreeing with any given statement. Results: Sisty-nine experts answered the questionnaire; a total of 30/39 statements achieved consensus. There was agreement on most of the statements. Time horizon to consider and costs were the issues on which no agreement was found. The results allowed the working group to define a list of good practices. Conclusion: While the structure and development of BIM are now well-known and well-applied at national level, there remains a great diversity of management of BIM tools at regional level. Consensus was reached among participating experts, as to the main characteristics, determinants and features of regional BIA/BIM in the perspective of the Italian payer.
Impact of Diﬀerent Physiotherapy Programs on Balance and Gait in Patients with Cerebral Infarction
Authors: Smailytė Paula , Lendraitienė Eglė , Žemaitienė Ieva 
Published: June 2023

Background. An ischemic stroke has long-term adverse effects on the physical, emotional, and social well-being of the stroke survivor, so it is very important to help the patient regain independence and lost functions as quickly and efficiently as possible. Dynamic Neuromuscular Stability exercises can restore movement patterns in these patients by simulating central nervous system.
The aim. To compare the effects of conventional physiotherapy and dynamic neuromuscular stabilization integrated into conventional physiotherapy on balance and gait in patients with cerebral infarction.
Methods. The study included 30 subjects who survived an ischemic stroke. Subjects were randomly divided into two groups of 15 people in each for different interventions: Conventional physiotherapy and Conventional physiotherapy combined with integrated dynamic neuromuscular stabilization. Subjects were examined at a baseline and after the interventions. Patients’ balance was assessed using the Biodex platform, which was chosen to evaluate fall risk, trunk stability, stability limits and sensory organization. Gait parameters (step length and step width) of the affected and unaffected leg were assessed using the Zebris FDM-T treadmill.
Results. Both conventional physiotherapy and physiotherapy with integrated dynamic neuromuscular stabilization improved gait parameters of the patients, as well as improving the results of the tests of fall risk, postural stability, stability limits, and the results of the sensory integration test when testing with eyes open on a stable surface (p<0.05). However, the results of the sensory integration tests while standing on a stable surface with eyes closed (p<0.001), with eyes open on an unstable surface (p<0.001) and with eyes closed on an unstable surface (p=0.021) improved only post physiotherapy with integrated dynamic neuromuscular stabilization.
Conclusions. Both conventional physiotherapy and physiotherapy with integrated dynamic neuromuscular stabilization significantly improved gait and balance parameters in stroke patients.
Keywords: Dynamic neuromuscular stabilization, stroke, balance, trunk stability, gait.
Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes
Authors: Pisani Silvia , Mauri Valeria , Negrello Erika , Mauramati Simone , Alaimo Gianluca , Auricchio Ferdinando , Benazzo Marco , Dorati Rossella , Genta Ida , Conti Bice , Ferretti Virginia Valeria , De Silvestri Annalisa , Pietrabissa Andrea , Marconi Stefania 
Published: June 2023

Introduction: The problem of organs’ shortage for transplantation is widely known: different manufacturing techniques such as Solvent casting, Electrospinning and 3D Printing were considered to produce bioartificial scaffolds for tissue engineering purposes and possible transplantation substitutes. The advantages of manufacturing techniques’ combination to develop hybrid scaffolds with increased performing properties was also evaluated.Methods: Scaffolds were produced using poly-L-lactide-co-caprolactone (PLA-PCL) copolymer and characterized for their morphological, biological, and mechanical features.Results: Hybrid scaffolds showed the best properties in terms of viability (&gt;100%) and cell adhesion. Furthermore, their mechanical properties were found to be comparable with the reference values for soft tissues (range 1–10 MPa).Discussion: The created hybrid scaffolds pave the way for the future development of more complex systems capable of supporting, from a morphological, mechanical, and biological standpoint, the physiological needs of the tissues/organs to be transplanted.
Levels and clinical significance of the m6A methyltransferase METTL14 in patients with coronary heart disease
Authors: Guo Fengxia , He Mei , Hu Bing , Li Gang 
Published: June 2023

ObjectiveTo investigate the association of methyltransferase-like protein 14 (METTL14) expression with coronary heart disease (CHD).MethodsThree hundred and sixteen patients who attended Henan Provincial People's Hospital between June 2019 and February 2021 with principal symptoms of pain or tightness in the chest and who underwent coronary angiography for definitive diagnosis were enrolled. The uric acid, TG, TC, LDL-C, HDL-C, apolipoprotein A1, free fatty acid, lipoprotein a, homocysteine, CRP, and SAA levels were examined. The levels of METTL14, TNF-α, MCP-1, VCAM-1, ICAM-1, and IL-6 were evaluated by ELISA.ResultsPatients with CHD had significantly higher m6A methyltransferase activity. In addition, the incidence of diabetes and hypertension, as well as the concentrations of TC, CRP, and SAA were higher in CHD patients. Patients with coronary lesion branches also had significantly increased TG, LDL-C, CRP, and SAA levels. TNF-α, MCP-1, VCAM-1, ICAM-1, and IL-6 expression was also markedly increased in the CHD group (P &lt; 0.001) as was the expression of METTL14 (P &lt; 0.001). The METTL14 expression levels also differed significantly in relation to the number of branches with lesions (P &lt; 0.01) and were correlated with SAA, VCAM-1, ICAM-1, IL-6, and the Gensini score. ROC curve analyses of METTL14 in CHD indicated an AUC of 0.881 (0.679, 0.894) with a cut-off value of 342.37, a sensitivity of 77%, and a specificity of 84%. MCP-1, VCAM-1, IL-6, SAA, and METTL14 were found to independently predict CHD risk.ConclusionsMETTL14 levels were found to be positively associated with inflammatory markers and to be an independent predictor of CHD risk.
Decreased IGF1R attenuates senescence and improves function in pancreatic β-cells
Authors: Iwasaki Kanako , Lalani Benjamin , Kahng Jiho , Carapeto Priscila , Sanjines Stephanie , Hela Francesko , Abarca Cristian , Tsuji Tadataka , Darcy Justin , Bartke Andrzej , Tseng Yu-Hua , Kulkarni Rohit N. , Aguayo-Mazzucato Cristina 
Published: June 2023

IntroductionThe enhanced β-cell senescence that accompanies insulin resistance and aging contributes to cellular dysfunction and loss of transcriptional identity leading to type 2 diabetes (T2D). While senescence is among the 12 recognized hallmarks of aging, its relation to other hallmarks including altered nutrient sensing (insulin/IGF1 pathway) in β-cells is not fully understood. We previously reported that an increased expression of IGF1R in mouse and human β-cells is a marker of older β-cells; however, its contribution to age-related dysfunction and cellular senescence remains to be determined.MethodsIn this study, we explored the direct role of IGF1R in β-cell function and senescence using two independent mouse models with decreased IGF1/IGF1R signaling: a) Ames Dwarf mice (Dwarf +/+), which lack growth hormone and therefore have reduced circulating levels of IGF1, and b) inducible β-cell-specific IGF1R knockdown (βIgf1rKD) mice.ResultsCompared to Dwarf+/- mice, Dwarf+/+ mice had lower body and pancreas weight, lower circulating IGF1 and insulin levels, and lower IGF1R and p21Cip1 protein expression in β-cells, suggesting the suppression of senescence. Adult βIgf1rKD mice showed improved glucose clearance and glucose-induced insulin secretion, accompanied by decreased p21Cip1 protein expression in β-cells. RNA-Seq of islets isolated from these βIgf1rKD mice revealed the restoration of three signaling pathways known to be downregulated by aging: sulfide oxidation, autophagy, and mTOR signaling. Additionally, deletion of IGF1R in mouse β-cells increased transcription of genes important for maintaining β-cell identity and function, such as Mafa, Nkx6.1, and Kcnj11, while decreasing senescence-related genes, such as Cdkn2a, Il1b, and Serpine 1. Decreased senescence and improved insulin-secretory function of β-cells were also evident when the βIgf1rKD mice were fed a high-fat diet (HFD; 60% kcal from fat, for 5 weeks).DiscussionThese results suggest that IGF1R signaling plays a causal role in aging-induced β-cell dysfunction. Our data also demonstrate a relationship between decreased IGF1R signaling and suppressed cellular senescence in pancreatic β-cells. Future studies can further our understanding of the interaction between senescence and aging, developing interventions that restore β-cell function and identity, therefore preventing the progression to T2D.
Global change explains reduced seeding in a widespread New Zealand tree: indigenous Tūhoe knowledge informs mechanistic analysis
Authors: Yukich Clendon Oscar M. M. , Carpenter Joanna K. , Kelly Dave , Timoti Puke , Burns Bruce R. , Boswijk Gretel , Monks Adrian 
Published: June 2023

IntroductionClimate change is expected to exacerbate the pressures faced by already fragile ecosystems. Negative impacts on the localized and culturally significant plant and animal species within these ecosystems will have cascading effects for the indigenous communities that interact with those species. Understanding how climate change affects culturally important seed crops may be particularly important, as seeds are critical for forest regeneration as well as providing sustenance for wildlife and people. In the central North Island/Te Ika-a-Māui of Aotearoa-New Zealand, Tūhoe elders of the Tuawhenua region have observed declines in seed production by the large-fruited locally dominant forest tree Beilschmiedia tawa (tawa, Lauraceae) over the last half century, which could be related to climate change.MethodsWe used seed trap data from six sites throughout the geographic range of tawa to measure trends in seed crop size from 1986 to 2020 and to determine which weather factors affect seed crops. We then used these weather predictors to hindcast how tawa seeding may have changed in Tuawhenua forests from 1910–2019, based on historic weather data.ResultsSeed trap data showed a decline in seeding through time across tawa’s range, and that seeding was lower at more northerly sites. Seed crops were synchronous among trees within sites, but were strongly asynchronous among sites. High seed crops were associated with cooler summer and winter temperatures, and with high rainfall. In the Tuawhenua region, increases in summer and winter temperatures appear to have contributed to the declines in tawa seed crops observed by elders, with the model predicting that years with heavy fruiting have become less frequent after 1940.DiscussionOur study provides strong evidence that tawa is undergoing changing seedfall patterns in response to changing climate. The biggest weather drivers of seeding that we identified in tawa were winter and summer temperature, both of which were negatively associated with crop size. Both winter and summer temperatures have increased in Tuawhenua in the last 100 years suggesting a possible mechanism to explain observations of long-term declines in tawa seedfall observed by Tūhoe elders of the Tuawhenua region, with ecological and cultural implications.
The relationship of cervicothoracic mobility restrictions to fall risk and fear of falling in ankylosing spondylitis
Authors: Johnston Janine L. , Harms Shane L. , Thomson Glen T. D. 
Published: June 2023

ObjectiveThe objective of this study is to determine whether restricted cervical mobility in ankylosing spondylitis (AS) is associated with increased fall frequency or fear of falling.MethodsA total of 134 AS patients and 199 age- and gender-matched control subjects (CS) with soft-tissue cervicothoracic pain were prospectively evaluated for fall risk. Subjects were divided into non-fallers, single fallers, and multiple fallers. Dynamic cervical rotations and static cervicothoracic axial measurements were compared between the groups. In total, 88 AS patients were reviewed more than once; Kaplan–Meier plots were constructed for fall risk as a function of cervical rotation amplitudes. Falls Efficacy Scale-International (FES-I) questionnaire measured the fear of falling.ResultsIn total, 34% of AS patients and 29% of CS fell (p = 0.271) in the year prior to evaluation. In AS, static anatomical measurements were unrelated to fall occurrence. The trends of multiple AS fallers to greater flexed forward postures and reduced dynamic cervical rotations were not statistically significant. Cervicothoracic pain (p = 0.0459), BASDAI (p = 0.002), and BASFI (p = 0.003) scores were greater in multiple fallers. FES-I scores were greater in fallers (p = 0.004). Of the 88 AS patients reviewed (or seen) on more than one occasion, 46.5% fell over the 9-year observation period, including all multiple fallers and 71.4% of single fallers. Survival curves showed increased fall risk as cervical rotational amplitudes decreased.ConclusionIn AS, decreased cervical rotations increase fall risk and fear of falling. In multiple fallers, falls were associated with greater disease activity. Cervical muscle stiffness in AS may cause non-veridical proprioceptive inputs and contribute to increased fall frequency similar to individuals with soft-tissue cervicothoracic pain.
Efficacy and safety of acupuncture on sleep quality for post-stroke insomnia: a systematic review and meta-analysis
Authors: Shi Menglong , Ji Zhaochen , Sun Tianye , Hu Haiyin , Chen Zhe , Feng Chaonan , Zhang Junhua , Zhao Min , Yang Fengwen 
Published: June 2023

BackgroundStroke is the second leading cause of death worldwide, and improving sleep quality in post-stroke insomnia is beneficial to the recovery of stroke. Acupuncture is widely used for the treatment of post-stroke insomnia in China. Therefore, this systematic review and meta-analysis were performed to explore the efficacy and safety of acupuncture for post-stroke insomnia.MethodsEight databases were searched from their inception to 12 September 2022. Two reviewers independently performed the study screening and data extraction. The outcomes include Pittsburgh Sleep Quality Index (PSQI), objective sleep data measured by polysomnography (PSG), long-term efficacy and adverse events. The quality of the trials was assessed by the Cochrane risk of bias tool 2.0. The RevMan 5.4 and Stata 15.1 were used for data synthesis.ResultsAmong 3,233 participants from 41 studies were included. Pooled results indicated that acupuncture was superior to control group (CG) in improving PSQI total score (standardized mean difference (SMD) = −1.03, 95% confidence interval (CI): −1.32, −0.74, P &lt; 0.00001), increasing sleep efficiency (SMD = 0.65, 95% CI: 0.37 to 0.92) and total sleep time (SMD = 0.54, 95% CI: 0.22 to 0.86). The favorable results in improving PSQI total score (SMD = 0.65, 95% CI: 0.37 to 0.92), reduced sleep latency (SMD = 1.84, 95% CI: 0.31 to 3.38) and increased total sleep time (SMD = −0.73, 95% CI: −1.15 to −0.31) were also observed in comparisons of acupuncture plus CG vs. CG. As of long-term efficacy and safety, the effects of acupuncture were long-term and robustness, however, due to limited safety information, reliable safety conclusions cannot be drawn. Subgroup analysis showed that acupuncture plus CG was superior to CG for post-infarction patients, but the efficacy of acupuncture alone compared to non-BZDs or other hypnotics needs further research. The GRADE assessment demonstrated that the level of evidence was mostly low or very low given the flaws in the study design and considerable heterogeneity among the included studies.ConclusionAcupuncture could improve sleep quality, has long-term efficacy and without serious adverse events. However, the findings should be treated with caution owing to the existence of methodological quality issues. More studies with rigorous designs are warranted for validation and explored the safety of acupuncture.
The BR signaling pathway regulates primary root development and drought stress response by suppressing the expression of PLT1 and PLT2 in Arabidopsis thaliana
Authors: Zhao Zhiying , Wu Shuting , Gao Han , Tang Wenqiang , Wu Xuedan , Zhang Baowen 
Published: June 2023

IntroductionWith the warming global climate, drought stress has become an important abiotic stress factor limiting plant growth and crop yield. As the most rapidly drought-sensing organs of plants, roots undergo a series of changes to enhance their ability to absorb water, but the molecular mechanism is unclear.Results and methodsIn this study, we found that PLT1 and PLT2, two important transcription factors of root development in Arabidopsis thaliana, are involved in the plant response to drought and are inhibited by BR signaling. PLT1- and PLT2-overexpressing plants showed greater drought tolerance than wild-type plants. Furthermore, we found that BZR1 could bind to the promoter of PLT1 and inhibit its transcriptional activity in vitro and in vivo. PLT1 and PLT2 were regulated by BR signaling in root development and PLT2 could partially rescue the drought sensitivity of bes1-D. In addition, RNA-seq data analysis showed that BR-regulated root genes and PLT1/2 target genes were also regulated by drought; for example, CIPK3, RCI2A, PCaP1, PIP1;5, ERF61 were downregulated by drought and PLT1/2 but upregulated by BR treatment; AAP4, WRKY60, and AT5G19970 were downregulated by PLT1/2 but upregulated by drought and BR treatment; and RGL2 was upregulated by drought and PLT1/2 but downregulated by BR treatment.DiscussionOur findings not only reveal the mechanism by which BR signaling coordinates root growth and drought tolerance by suppressing the expression of PLT1 and PLT2 but also elucidates the relationship between drought and root development. The current study thus provides an important theoretical basis for the improvement of crop yield under drought conditions.
“Diminishing returns” and leaf area-biomass scaling of ferns in subtropical ecosystems
Authors: Chen Shubing , Li Jinlong , Sun Jun , Zhong Quanlin , Hu Dandan , Cheng Dongliang 
Published: June 2023

Foliage leaves are the primary photosynthetic organ of the majority of vascular plants, and their area vs. biomass scaling relationships provide valuable insights into the capacity and investment in light interception, which is critical to plant growth and performance. The “diminishing returns” hypothesis (DRH), which is based primarily on data from gymnosperms and angiosperms, posits that leaf (lamina) area scales with leaf dry mass. on average with a scaling exponent less than 1.0. However, it remains uncertain whether DRH applies to ferns or whether ecological factors affect the scaling exponents governing fern leaf morphometrics. To address this issue, 182 individuals of 28 subtropical ferns species were studied at low, medium, and high elevations (i.e., 600 m, 900 m, and 1200 m, respectively) in Mount Wuyi National Park, Jiangxi Province, China. The scaling relationships between leaf area and leaf biomass for individual and total leaf of ferns at different elevations were examined by using standardized major axis regression protocols. Analyses of the 28 fern species (using Blomberg K-value protocols) indicated no phylogenetic biases among the species compositions of the three different elevations. In addition, at the individual plant level, individual leaf area (ILA) did not differ significantly among the three different elevations (P &gt; 0.05). However, individual leaf mass (ILM) was significantly higher at 900m than at 1200m (P &lt; 0.05), resulting in a significantly higher leaf mass per area (LMA) at the 900m elevation than at the 600m and 1200m elevations (P &lt; 0.05). The ILA and ILM at the 900m elevation were significantly higher than at the 600m elevation (P &lt; 0.05). At the species level, ILA and ILM did not differ significantly among the three elevations (P &gt; 0.05). The total leaf area per individual (TLA) did not differ significantly across the different elevations (P &gt; 0.05). However, total leaf mass per individual (TLM) did differ significantly (P &lt; 0.05). At the individual plant level, the scaling exponents for ILA vs. ILM and TLA vs. TLM at the three different elevations were all significantly less than 1.0 (P &lt; 0.05), which was consistent with the DRH. At the species level, the scaling exponents for the ILA vs. ILM were significantly less than 1.0 at the middle and high elevations, but not at the low elevation. The scaling exponents of the TLA and TLM were numerically highest in the middle elevation, and all were less than 1.0 for the three elevations. These results indicate that the scaling relationships of leaf area versus mass of subtropical ferns at different elevations support the DRH hypothesis. The study further informs our understanding of the resource allocation strategies of an ancient and diverse plant lineage.