bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Application of Mealworm (Tenebrio molitor) Oil in Cosmetic Formulations
Authors: Verheyen Geert R. , Meersman Filip , Noyens Isabelle , Goossens Sarah , Van Miert Sabine 
Published: January 2023

AbstractThe depletion of natural resources, the increasing use of valuable land surface for human activities, and the generation of waste are detrimental to the planet. Sustainable alternatives to guarantee a sufficient supply of food, feed, and biomaterials are needed. Insects may hold an interesting position in a circular economy to tackle contemporary societal challenges. Using insects for food, feed, biomaterial production, and to valorize side‐streams, have gained increased interest. Mealworms are amenable to large‐scale farming. Herein, the potential of mealworm oil for implementation in a cosmetic hand cream is evaluated. It is shown that mealworms are rich in oil (≈30% of the dry weight), and the oil can be extracted using hexane or ethyl acetate as a solvent. The euthanization step of mealworms has profound effect on the free fatty acid content. The fatty acid profile indicates a large proportion of unsaturated fatty acids, which is promising for use in cosmetic applications. In a proof of concept, the mealworm oil is implemented in a hand cream and compared to hand cream containing macadamia nut oil. No differences in odor and aspect/stability are observed, but mealworm creams are slightly less white color. Mealworm oil is suitable for cosmetics applications.Practical applications: Several insect species can be sustainably reared on low‐value organic side‐streams, requiring only small amounts of land‐surface, less water, and producing less greenhouse gases. In addition, they have short life cycles and can be reared continuously and locally. This implies that insects are an interesting source of biological materials including proteins and oils. Insect oils, including oil of mealworms, have interesting fatty acid profiles that make them amenable to replace oils of vegetable origin for applications within cosmetics. The results indicate that mealworm oil can be a novel, sustainable ingredient for use in the cosmetics industry.
Synthesis of Bifunctional Lipoxin‐Derived Enzyme‐Triggered CO‐Releasing Molecules (LipET‐CORMs)
Authors: Hemmersbach Lars , Adam Ruth , Plevnali Christina , Zhang Xinmiao , Yard Benito , Schmalz Hans‐Günther 
Published: January 2023

AbstractIn an attempt to develop new anti‐inflammatory agents which act by co‐release of carbon monoxide (CO) and a specialized pro‐resolving mediator, we designed conjugates of a lipoxin A4 analogue and an acyloxycyclohexadiene‐Fe(CO)3 complex as an esterase‐triggered CO‐releasing molecule (ET‐CORM). After adjustment of the protecting group strategy, two of such compounds were successfully prepared by total synthesis (12 steps; 4–5 % overall yield) starting from deoxy‐d‐ribose and exploiting a Wittig olefination and an intermolecular Heck reaction as key C−C bond‐forming steps. A crucial late reduction of an aryl‐ketone moiety in the presence of a highly sensitive dienol ester functionality was achieved with BH3‐SMe2 in the presence of catalytic amounts of NaBH4. Both target compounds were dose‐dependently toxic towards cultured human umbilical vein endothelial cells (HUVEC), with LipET‐CORM 1‐A being slightly more toxic. While induction of heme oxygenase 1 (HO‐1) in HUVEC was observed for both compounds, they did not inhibit TNF‐α‐mediated VCAM‐1 expression in these cells. In M2 polarized macrophages HO‐1 expression was more pronounced as compared to M1 polarized macrophages. In both types of macrophages HO‐1 expression was downregulated by lipopolysaccharide, but only in M2 macrophages HO‐1 expression was rescued by LipET‐CORM. 15‐Lipoxygenase (15‐LO) was only expressed in M2 macrophages and was not influenced by LipET‐CORM. Collectively our data demonstrate that LipET‐CORMs induce HO‐1 expression in endothelial cells and M2 polarized macrophages. The role of the intra‐cellular released lipoxin A4 in resolution of inflammation, however, remains to be assessed.
Failure mechanism and divisional differentiated control of surrounding rock in mining roadway under remaining coal pillar in close‐distance coal seam
Authors: Chen Dong‐dong , Ma Xiang , Wu Yi‐yi , Xie Sheng‐rong , Li Hui , Jiang Zai‐sheng , Wang En , Guo Fang‐fang , Guo Wen‐ke , Ye Qiu‐cheng 
Published: January 2023

AbstractRegarding the issue of intense mining pressure appearing in the underlying gateway below the remaining coal pillar in the close‐distance coal seam (the remaining coal pillar is perpendicular to the underlying section coal pillar), 401 working face is used as the engineering background. Field measurements, laboratory experiments, numerical simulations, and engineering verification techniques are used to study the abutment pressure's evolution properties and the plastic zone's propagation laws before and after the underlying coal seam roadway experienced the mining impact. The conclusions are as follows: ① The maximum plastic area on the two sides and the roof of the roadway underlying the gob are up to 2 and 1.5 m, whereas the maximum plastic area on the two sides and the roof of the roadway underlying the remaining coal pillar are up to 5 and 4.5 m, respectively. Moreover, the plastic area extends along the two sides, and the section coal pillar is completely broken when the working face is mined below the remaining coal pillar. ② The stress increase coefficient K in the overlap area of the remaining coal pillar and the underlying section coal pillar reaches 3.4 when the mining face penetrates the underlying remaining coal pillar and the advance abutment pressure is overlaid with the concentrated stress of the coal pillar. ③ When the underlying working face is mined to 4, −2, −8, and −14 m away from the remaining coal pillar, the damage range of the roadway 5–10 m ahead increases in turn. At the same time, the maximum plastic area of the roof passes through the plastic area of the upper coal seam floor. Therefore, the underlying and transition areas on both sides of the remaining coal pillar are divided into Area I (15 m) → Area II (the most complicated area to control under the remaining coal pillar, 20 m) → Area III (25 m) according to the width. Furthermore, the divisional differentiated combined control technology of channel steel truss anchor cable with joint double‐way locking control function of roof and coal pillar in Areas I and III, while channel steel truss anchor cable with joint double‐way locking control function of roof and side + high resistance integral door‐type support is proposed in Area II. Field engineering practice shows that the deformation of the roadway surrounding rock can be controlled within 210 mm after adopting the above divisional combined control technology. Finally, the mining operation can safely and efficiently pass through the remaining coal pillar. The research results have important reference values for surrounding rock control of mining roadways in the overlapping area of similar “+”‐type cross‐working face.
Updating the Chronic Freshwater Ecotoxicity Database and Biotic Ligand Model for Nickel for Regulatory Applications in Europe
Authors: Peters Adam , Nys Charlotte , Leverett Dean , Wilson Iain , Van Sprang Patrick , Merrington Graham , Middleton Elizabeth , Garman Emily , Schlekat Christian 
Published: January 2023

Abstract
Bioavailability has been taken into account in the regulation of nickel in freshwater ecosystems in Europe for over a decade; during that time a significant amount of new information has become available covering both the sensitivity of aquatic organisms to nickel toxicity and bioavailability normalization. The ecotoxicity database for chronic nickel toxicity to freshwater organisms has been updated and now includes 358 individual data points covering a total of 53 different species, all of which are suitable for bioavailability normalization to different water chemistry conditions. The bioavailability normalization procedure has also been updated to include updates to the bioavailability models that enable more sensitive water chemistry conditions to be covered by the model predictions. The updated database and bioavailability normalization procedure are applicable to more than 95% of regulated European surface water conditions and have been used to calculate site-specific criteria for a variety of different water chemistry scenarios, to provide an indication of how the sensitivity to nickel varies between different water types. The hazardous concentration for 5% of a species (HC5) values for this diverse selection of water types range from 1.6 to 36 µg L–1, clearly demonstrating the importance of accounting for nickel bioavailability in freshwaters. This updated database and bioavailability normalization procedure provide a robust basis for the derivation of regulatory thresholds for chronic nickel toxicity in freshwaters such as predicted no-effect concentrations and Environmental Quality Standards and are protective of the results of several mesocosm studies. Environ Toxicol Chem 2023;42:566–580. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Assessment of high‐order IMEX methods for incompressible flow
Authors: Guesmi Montadhar , Grotteschi Martina , Stiller Jörg 
Published: January 2023

SummaryThis paper investigates the competitiveness of semi‐implicit Runge‐Kutta (RK) and spectral deferred correction (SDC) time‐integration methods up to order six for incompressible Navier‐Stokes problems in conjunction with a high‐order discontinuous Galerkin method for space discretization. It is proposed to harness the implicit and explicit RK parts as a partitioned scheme, which provides a natural basis for the underlying projection scheme and yields a straight‐forward approach for accommodating nonlinear viscosity. Numerical experiments on laminar flow, variable viscosity and transition to turbulence are carried out to assess accuracy, convergence and computational efficiency. Although the methods of order 3 or higher are susceptible to order reduction due to time‐dependent boundary conditions, two third‐order RK methods are identified that perform well in all test cases and clearly surpass all second‐order schemes including the popular extrapolated backward difference method. The considered SDC methods are more accurate than the RK methods, but become competitive only for relative errors smaller than ca .
The geopolitical risk premium in the commodity futures market
Authors: Cheng Daxuan , Liao Yin , Pan Zheyao 
Published: January 2023

AbstractIn this study, we investigate the geopolitical risk premium in the commodity futures market. By estimating the exposure of cross‐sectional commodity futures excess returns on a historical geopolitical risk index, we find that commodities with low‐risk betas generate 9.05% higher annual risk‐adjusted returns than those with high‐risk betas. The results indicate that low‐geopolitical‐risk‐related commodity futures contracts require extra compensation by risk‐averse investors due to hedging demand. We also explore the time‐varying characteristics of the geopolitical risk premium: It appears more pronounced during high‐geopolitical‐risk periods and before the year 2000. Finally, we exploit the subcategories of geopolitical risk and find that geopolitical threats better explain the variation of the geopolitical risk premium than do geopolitical acts, making it a main source of the geopolitical risk premium.
Analysis of ecological health status of the Muni Lagoon: Evidence from heavy metal content in its water and fish samples
Authors: Okyere Emmanuel Yeboah , Adu‐Boahen Kofi , Boateng Isaac , Dadson Ishmael Yaw , Boanu Nelson Yeboah , Kyeremeh Sender 
Published: January 2023

AbstractThe study examined the ecological health status of the Muni lagoon amidst increasing development in and around its catchment using a concentration of heavy metals in its water and fish samples as a proxy. Flame atomic absorption spectrophotometer (FAAS) laboratory analysis was employed to determine heavy metals (cadmium [Cd], lead [Pb], iron [Fe], manganese [Mn] and zinc) present in water and fish samples within the Muni Lagoon. The study revealed that the Muni Lagoon and feeder rivers were polluted with heavy metals (Fe = 0.453, Cd = 0.201, Mn = 0.105 and Pb = 0.024) comparing their concentrations with the US Environmental Protection Agency (USEPA) and Water Resources Commission (WRC) limit. Fish within the lagoon were found to pose no harm to consumers as traces of heavy metal concentrations were below the Food and Agriculture Organisation of the United Nations/World Health Organisation maximum permissible limit. The study further revealed that the application of agricultural inputs such as fertilisers, pesticides and domestic waste as well as unregulated gutter channels were the major source of heavy metals. A paired sample t‐test showed a statistically significant difference between the wet and dry season's concentrations of cadmium and lead. For cadmium, the t‐test found t(5) = −7.265; p = .001 between the wet season's concentration and the dry season's concentration and for lead, the t‐test found t(5) = 5.061, p = .004 between the wet and dry season concentrations in the lagoon. It is therefore recommended that the Forestry Commission, Municipal Assembly and the local leaders should collaborate in regulating activities occurring in and around the catchment of the lagoon.
Deficient deposition of new myelin impairs adult optic nerve function in a murine model of diabetes
Authors: Wu Haoqian , Chen Xianjun , Yu Bin , Zhang Jieqiong , Gu Xingmei , Liu Wei , Mei Feng , Ye Jian , Xiao Lan 
Published: January 2023

AbstractVisual impairment in diabetes is a growing public health concern. Apart from the well‐defined diabetic retinopathy, disturbed optic nerve function, which is dependent on the myelin sheath, has recently been recognized as an early feature of visual impairment in diabetes. However, the underlying cellular mechanisms remain unclear. Using a streptozotocin‐induced diabetic mouse model, we observed a myelin deficiency along with a disturbed composition of oligodendroglial lineage cells in diabetic optic nerve. We found that new myelin deposition, a continuous process that lasts throughout adulthood, was diminished during pathogenesis. Genetically dampening newly generated myelin by conditionally deleting olig2 in oligodendrocyte precursor cells within this short time window extensively delayed the signal transmission of the adult optic nerve. In addition, clemastine, an antimuscarinic compound that enhances myelination, significantly restored oligodendroglia, and promoted the functional recovery of the optic nerve in diabetic mice. Together, our results point to the role of new myelin deposition in optic neuropathy under diabetic insult and provide a promising therapeutic target for restoring visual function.
Disentangling poststroke cognitive deficits and their neuroanatomical correlates through combined multivariable and multioutcome lesion‐symptom mapping
Authors: Weaver Nick A. , Mamdani Muhammad Hasnain , Lim Jae‐Sung , Biesbroek Johannes Matthijs , Biessels Geert Jan , Huenges Wajer Irene M. C. , Kang Yeonwook , Kim Beom Joon , Lee Byung‐Chul , Lee Keon‐Joo , Yu Kyung‐Ho , Bae Hee‐Joon , Bzdok Danilo , Kuijf Hugo J. 
Published: January 2023

AbstractStudies in patients with brain lesions play a fundamental role in unraveling the brain's functional anatomy. Lesion‐symptom mapping (LSM) techniques can relate lesion location to cognitive performance. However, a limitation of current LSM approaches is that they can only evaluate one cognitive outcome at a time, without considering interdependencies between different cognitive tests. To overcome this challenge, we implemented canonical correlation analysis (CCA) as combined multivariable and multioutcome LSM approach. We performed a proof‐of‐concept study on 1075 patients with acute ischemic stroke to explore whether addition of CCA to a multivariable single‐outcome LSM approach (support vector regression) could identify infarct locations associated with deficits in three well‐defined verbal memory functions (encoding, consolidation, retrieval) based on four verbal memory subscores derived from the Seoul Verbal Learning Test (immediate recall, delayed recall, recognition, learning ability). We evaluated whether CCA could extract cognitive score patterns that matched prior knowledge of these verbal memory functions, and if these patterns could be linked to more specific infarct locations than through single‐outcome LSM alone. Two of the canonical modes identified with CCA showed distinct cognitive patterns that matched prior knowledge on encoding and consolidation. In addition, CCA revealed that each canonical mode was linked to a distinct infarct pattern, while with multivariable single‐outcome LSM individual verbal memory subscores were associated with largely overlapping patterns. In conclusion, our findings demonstrate that CCA can complement single‐outcome LSM techniques to help disentangle cognitive functions and their neuroanatomical correlates.
Alternative Synthesis of 5‐(1H‐Pyrazol‐4‐yl)‐2‐{6‐[(2,2,6,6‐tetramethylpiperidin‐4‐yl)oxy]pyridazin‐3‐yl}phenol
Authors: Zhou Jianguang , Gu Xingxian , Yao Shuping , Ye Jiong , Fu Peng , Liu Siqian , Dedic Darija , Gallou Fabrice 
Published: January 2023

AbstractA scalable and environmentally benign route to the free base of branaplam, a small molecule splicing modulator, was identified and developed. This alternative approach circumvented the inherent risk of dioxin formation associated with ortho‐halo phenol derivatives present in the previous route. A Friedel–Crafts approach provided a reliable access to the key intermediate, subsequently followed by a Suzuki–Miyaura cross‐coupling. Multiple process aspects of the synthetic approach were evaluated, and a robust process for its large‐scale synthesis was developed and successfully demonstrated.
Impact of land cover changes on Long‐Term Regional‐Scale groundwater recharge simulation in cold and humid climates
Authors: Dubois Emmanuel , Larocque Marie , Brunner Philip 
Published: January 2023

AbstractIn cold and humid climates, warming temperatures will result in longer growing seasons, leading to land cover changes that could have long‐term impacts on groundwater recharge (GWR), in addition to the direct impacts of climate change. The objective of this study was therefore to investigate whether land cover (LC) changes need to be considered when simulating long‐term regional‐scale potential GWR in cold and humid climates by (1) quantifying how LC changes impact simulated GWR and (2) quantifying the combined impacts of LC and climate changes on the future GWR changes. Using the region of southern Quebec (Canada) as a case study and a water budget model, this work proposes an innovative coupling of land cover change scenarios and specific future climate conditions to simulate spatially distributed transient GWR over the 1951–2100 period. The results showed that including LC changes in long‐term GWR simulations produced statistically significant increases in GWR compared to using a constant LC through time (average of +13 mm). Massive afforestation taking place on agricultural lands simulated for one of the scenario chains (RCP4.5) increased GWR by reducing runoff during the snow‐dominated period (average − 17 mm). The results also showed that GWR was more sensitive to climate change for scenarios that included intense land cover changes. Additionally, the spatial distribution of the LC changes influenced their simulated impacts on GWR. Considering that the methodology was computationally feasible and entirely transferrable to the new CMIP6 ensemble, LC changes should be considered systematically in long‐term groundwater resources simulations.
CTLA‐4 inhibition facilitates follicular T and B cell interaction and the production of tumor‐specific antibodies
Authors: Jo Areum , Jeong Dasom , Eum Hye Hyeon , Kim Nayoung , Na Minsu , Kang Huiram , Lee Hae‐Ock 
Published: January 2023

AbstractImmune checkpoint inhibitors (ICIs) induce activation and expansion of cytotoxic T cells. To depict a comprehensive immune cell landscape reshaped by the CTLA‐4 checkpoint inhibitor, we performed single‐cell RNA sequencing in a mouse syngeneic tumor transplant model. After CTLA‐4 inhibition, tumor regression was accompanied by massive immune cell expansion, especially in T and B cells. We found that B cells in tumor transplant represented follicular, germinal center and plasma B cells, some of which shared identical B cell receptor clonotypes and possessed tumor reactivity. Furthermore, the posttreatment tumor contained a tertiary lymphoid‐like structure with intermingled T and B cells. These data suggest germinal center formation within the tumor mass and in situ differentiation of tumor‐specific plasma cells. Taken together, our data provide a panoramic view of the immune microenvironment after CTLA‐4 inhibition and suggest a role for tumor‐specific B cells in antitumor immunity.
Tumorigenic activation around HPV integrated sites in head and neck squamous cell carcinoma
Authors: Mima Masato , Okabe Atsushi , Hoshii Takayuki , Nakagawa Takuya , Kurokawa Tomoya , Kondo Satoru , Mizokami Harue , Fukuyo Masaki , Fujiki Ryoji , Rahmutulla Bahityar , Yoshizaki Tomokazu , Hanazawa Toyoyuki , Misawa Kiyoshi , Kaneda Atsushi 
Published: January 2023

AbstractHuman papillomavirus (HPV) is causally involved in the development of head and neck squamous cell carcinoma (HNSCC). The integration of HPV drives tumorigenesis through expression of oncogenic viral genes as well as genomic alterations in surrounding regions. To elucidate involvement of epigenetic dysregulation in tumorigenesis, we here performed integrated analyses of the epigenome, transcriptome and interactome using ChIP‐seq, RNA‐seq and Hi‐C and 4C‐seq for HPV(+) HNSCCs. We analyzed clinical HNSCC using The Cancer Genome Atlas data and found that genes neighboring HPV integration sites were significantly upregulated and were correlated with oncogenic phenotypes in HPV(+) HNSCCs. While we found four HPV integration sites in HPV(+) HNSCC cell line UPCI‐SCC‐090 through target enrichment sequencing, 4C‐seq revealed 0.5 to 40 Mb of HPV‐interacting regions (HPVIRs) where host genomic regions interacted with integrated HPV genomes. While 9% of the HPVIRs were amplified and activated epigenetically forming super‐enhancers, the remaining non‐amplified regions were found to show a significant increase in H3K27ac levels and an upregulation of genes associated with GO terms, for example, Signaling by WNT and Cell Cycle. Among those genes, ITPR3 was significantly upregulated, involving UPCI‐SCC‐090‐specific super‐enhancer formation around the ITPR3 promoter and in the 80‐kb‐downstream region. The knockdown of ITPR3 by siRNA or CRISPR deletions of the distant enhancer region led to a significant suppression of cell proliferation. The epigenetic activation of HPVIRs was also confirmed in other cell lines, UM‐SCC‐47 and UM‐SCC‐104. These data indicate that epigenetic activation in HPVIRs contributes, at least partially, to genesis of HPV(+) HNSCC.