bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progressive scoliosis associated with microphthalmia with limb anomalies: A case report
Authors: Yoshikawa Yoshiro , Yamakawa Chikashi , Shimabukuro Takanao , Kinjo Hideo , Fukase Shogo , Oshiro Hiromichi , Katsuki Ryo , Tome Yasunori , Nishida Kotaro 
Published: March 2023

Rationale:
Microphthalmia with limb anomalies is a rare, autosomal recessive, multiple congenital anomaly syndrome. Patients with this syndrome particularly present with monocular or bilateral anophthalmia/microphthalmia and distal limb anomalies. However, details regarding associated spinal deformities have not been fully elucidated.

Patient concerns:
A 12-year-old girl initially presented with progressive scoliosis, who was previously diagnosed with microphthalmia with limb anomalies. However, 4 years after the initial visit, the scoliosis deformity gradually progressed. The patient and family requested the surgical treatment to preserve standing/sitting balance.

Diagnoses:
She was diagnosed with microphthalmia with limb anomalies and progressive scoliosis.

Interventions:
A posterior corrective fusion surgery (including a pelvic fusion) was performed to prevent future standing/sitting imbalance.

Outcomes:
Significant improvement of spinal deformity was observed, with no adverse events.

Lessons:
This report demonstrated a case of progressive scoliosis associated with microphthalmia with limb anomalies. A posterior corrective spinal fusion was effective to preserve standing/sitting balance. To the best of our knowledge, this is the first report of surgical treatment of progressive scoliosis associated with microphthalmia with limb anomalies.
Risk Factors of Cancer in Pediatric-Onset Inflammatory Bowel Disease in Denmark and Finland
Authors: Malham Mikkel , Jansson Sabine , Malmborg Petter , Olén Ola , Paerregaard Anders , Virta Lauri J. , Jakobsen Christian , Kolho Kaija-Leena , Wewer Vibeke 
Published: March 2023

Objectives:
Pediatric-onset inflammatory bowel disease (pIBD) increases the risk of developing several different cancer forms. In this case-control study, we aimed to assess the impact of medical treatment and disease activity on the risk of developing disease-associated cancer (DAC) and treatment-associated cancer (TAC).

Methods:
In a previous study, we identified 27 cases of DAC (colorectal cancer, small bowel cancer, and cholangiocarcinoma) and 28 TAC (lymphoma and skin cancer) in 6689 patients with pIBD in Denmark and Finland during the period 1992–2015. In this study, the patient charts were reviewed manually. Cancer-free patients from another population-based pIBD cohort were included as controls. We recorded data on phenotype, medical treatment, surgery, and relapses. Logistic regression was used to estimate adjusted odds ratios (aOR) with 95% confidence intervals (95% CI) to estimate the relative risk.

Results:
We included 16 cases with DAC, 21 with TAC, and 331 controls. For DAC, lower frequencies of IBD-relapses were associated with an increased risk of cancer (OR 0.2 [95% CI: 0.04–0.8]). For TAC, we found an increased risk in patients receiving thiopurines at any point during the follow-up period (aOR: 11.7 [95% CI: 2.1–116.2]) and an association with proportion of follow-up time being exposed to thiopurines (aOR 5.6 [95% CI: 1.1–31.5]).

Conclusions:
In this nation-wide study, covering all pIBD patients from Denmark and Finland, we found that pIBD patients treated with thiopurines had an increased risk of TAC.
A Regional Approach to Hospital-Based Violence Intervention Programs Through LOV
Authors: Mueller Kristen L. , Chapman-Kramer Kateri , Cooper Benjamin P. , Kaser Taylor , Mancini Michael , Moran Vicki , Vogel Matthew , Foraker Randi E. , Anwuri Victoria 
Published: March 2023

Context:
Community violence is an underaddressed public health threat. Hospital-based violence intervention programs (HVIPs) have been used to address the root causes of violence and prevent reinjury.

Objective:
In this article, we describe the methodology of the St Louis Region-wide HVIP, Life Outside Violence (LOV) program, and provide preliminary process outcomes.

Design:
Life Outside Violence mentors intervene following a violent injury to decrease risk of subsequent victimization and achieve goals unique to each participant by providing therapeutic counseling and case management services to patients and their families.

Participants and Setting:
Eligible patients are victims of violent injury between the ages of 8 and 24 years, who are residents of St Louis, Missouri, and present for care at a LOV partner adult or pediatric level I trauma hospital.

Intervention:
Enrolled participants receive program services for 6 to 12 months and complete an individual treatment plan.

Main Outcome Measures:
In this article, we report LOV operational methodology, as well as process metrics, including program enrollment, graduation, and qualitative data on program implementation.

Results:
From August 15, 2018, through April 30, 2022, 1750 LOV-eligible violently injured patients presented to a partner hospital, 349 were approached for program enrollment, and 206 consented to enroll in the program. During this pilot phase, 91 participants graduated from the LOV program and have process output data available for analysis.

Conclusions:
Life Outside Violence has been implemented into clinical practice as the first HVIP to influence across an entire region through partnership with multiple university and hospital systems. It is our hope that methods shared in this article will serve as a primer for organizations hoping to implement and expand HVIPs to interrupt community violence at the regional level.
Mapping the Central Sulcus Extraoperatively Using Stereoelectroencephalography: A New Application of an Established Method
Authors: Kheder Ammar , Al-Ramadhani Ruba , Bhalla Sonam , Dickey Adam , Alwaki Abdulrahman , Chern Joshua , Pederson Nigel , Gedela Satyanarayana 
Published: March 2023

Purpose:
Central sulcus localization is undertaken intraoperatively with subdural electrodes through a phase reversal technique using somatosensory evoked potentials from sensorimotor cortices. Extraoperative central sulcus localization using stereoelectroencephalography has not been described previously.

Methods:
Six pediatric patients (aged 12–18 years, 50% females) were investigated with stereoelectroencephalography. Peripheral median and posterior tibial nerve stimulation were performed while recording somatosensory evoked potentials from stereoelectroencephalography electrodes.

Results:
Central sulcus was successfully localized by this novel method, and this was further supplemented by cortical stimulation data.

Conclusions:
This is the first report of somatosensory evoked potentials gained using stereoelectroencephalography in primary motor and sensory cortices. This can further supplement other data for safe surgical resection in the eloquent cortex.
Learning to fake it: limited responses and fabricated references provided by ChatGPT for medical questions
Authors: Gravel Jocelyn , D’Amours-Gravel Madeleine , Osmanlliu Esli 
Published: March 2023

AbstractBackgroundChatGPT have gained public notoriety and recently supported manuscript preparation. Our objective was to evaluate the quality of the answers and the references provided by ChatGPT for medical questions.MethodsThree researchers asked ChatGPT a total of 20 medical questions and prompted it to provide the corresponding references. The responses were evaluated for quality of content by medical experts using a verbal numeric scale going from 0 to 100%. These experts were the corresponding author of the 20 articles from where the medical questions were derived. We planned to evaluate three references per response for their pertinence, but this was amended based on preliminary results showing that most references provided by ChatGPT were fabricated.ResultsChatGPT provided responses varying between 53 and 244 words long and reported two to seven references per answer. Seventeen of the 20 invited raters provided feedback. The raters reported limited quality of the responses with a median score of 60% (1stand 3rdquartile: 50% and 85%). Additionally, they identified major (n=5) and minor (n=7) factual errors among the 17 evaluated responses. Of the 59 references evaluated, 41 (69%) were fabricated, though they appeared real. Most fabricated citations used names of authors with previous relevant publications, a title that seemed pertinent and a credible journal format.InterpretationWhen asked multiple medical questions, ChatGPT provided answers of limited quality for scientific publication. More importantly, ChatGPT provided deceptively real references. Users of ChatGPT should pay particular attention to the references provided before integration into medical manuscripts.
Long-Term Outcomes After Transcatheter Aortic Valve Replacement: Meta-Analysis of Kaplan-Meier-Derived Individual Patient Data
Authors: Shimamura Junichi , Takemoto Sho , Fukuhara Shinichi , Miyamoto Yoshihisa , Yokoyama Yujiro , Takagi Hisato , Kampaktsis Polydoros N , Kolte Dhaval , Grubb Kendra J , Kuno Toshiki , Latib Azeem 
Published: March 2023

AbstractBackgroundTranscatheter aortic valve replacement (TAVR) is as an alternative treatment to surgical AVR, but the long-term outcomes of TAVR remain unclear. This study aimed to analyze long-term outcomes following TAVR using meta-analysis.MethodsA literature search was performed with MEDLINE, EMBASE, Cochrane Library, Web of Science, and Google Scholar through December 2022; studies reporting clinical outcomes of TAVR with follow-up periods of ≥ 8 years were included. The outcomes of interest were overall survival and/or freedom from structural valve deterioration (SVD). Surgical risk was assessed with the Society of Thoracic Surgeons (STS) predicted risk of mortality (PROM) score. A subgroup analysis was conducted for intermediate-/high-surgical risk patients only.ResultsEleven studies including 5,458 patients were identified and analyzed. The mean age was 82.0 ± 6.5 years, and mean STS PROM score ranged from 2.9 to 10.6%. Survival rate at 5 and 10 years was 47.7% ± 1.4% and 12.1 ± 2.0%. Five studies including 1,509 patients were analyzed for SVD. Freedom from SVD at 5 and 8 years was 95.5 ± 0.7% and 84.9 ± 3.1%. Similar results for survival and SVD were noted in the subgroup analysis of intermediate-/high-risk patients.ConclusionsFollowing TAVR, approximately 88% of patients died within 10 years, whereas 85% were free from SVD at 8 years. These date suggest that baseline patient demographic have the greatest impact on survival, and SVD does not seem to have a prognostic impact in this population. Further investigations on longer-term outcomes of younger and lower-risk patients are warranted.What is new?-Meta-analysis of time-to-event data was performed to assess long-term outcomes following transcatheter aortic valve replacement in a large population.-Six studies, including 4,910 patients with follow-up periods of more than 20 years were identified and analyzed.-Nearly 88% of patients who underwent transcatheter aortic valve replacement died within 10 years, while 85% remained free from structural valve deterioration at 8 years.Perspective Statement What are the clinical implications?-This meta-analysis demonstrated excellent freedom from structural valve deterioration in intermediate- to high elderly risk patients.-Baseline patient characteristics correlated with high mortality at 10 years.-Further investigation into the outcomes in younger and lower-risk populations is imperative to evaluate the future expanded indications for transcatheter aortic valve replacement.
Her6 and Prox1 are novel regulators of photoreceptor regeneration in the zebrafish retina
Authors: Veen Kellie , Krylov Aaron , Yu Shuguang , He Jie , Boyd Patrick , Hyde David R. , Mantamadiotis Theo , Cheng Louise Y , Jusuf Patricia R 
Published: March 2023

AbstractDamage to light-sensing photoreceptors (PRs) occurs in highly prevalent retinal diseases. As humans cannot regenerate new PRs, these diseases often lead to irreversible blindness. Intriguingly, animals, such as the zebrafish, have the ability to regenerate PRs efficiently and restore functional vision. Upon injury, mature Müller glia (MG) undergo reprogramming to adopt a stem cell-like state. This process is similar to cellular dedifferentiation, and results in the generation of progenitor cells, which, in turn, proliferate and differentiate to replace lost retinal neurons. In this study, we tested whether factors involved in dedifferentiation ofDrosophilaCNS are implicated in the regenerative response in the zebrafish retina. We found thathairy-related 6(her6) negatively regulates of PR production by regulating the rate of cell divisions in the MG-derived progenitors.prospero homeobox 1(prox1) is expressed in differentiated PRs, and likely promotes PR differentiation through phase separation. Interestingly, upon Her6 downregulation, Prox1 is precociously upregulated in the PRs, to promote PR differentiation; conversely, loss of Prox1 also induces a downregulation of Her6. Together, we identified two novel candidates of PR regeneration that cross regulate each other, and may be exploited to promote human retinal regeneration and vision recovery.
THE RHODOEXPLORER PLATFORM FOR RED ALGAL GENOMICS AND WHOLE GENOME ASSEMBLIES FOR SEVERAL GRACILARIA SPECIES
Authors: Lipinska Agnieszka P. , Krueger-Hadfield Stacy A. , Godfroy Olivier , Dittami Simon , Ayres-Ostrock Lígia , Bonthond Guido , Brillet-Guéguen Loraine , Coelho Susana , Corre Erwan , Cossard Guillaume , Destombe Christophe , Epperlein Paul , Faugeron Sylvain , Ficko-Blean Elizabeth , Beltrán Jessica , Lavaut Emma , Bars Arthur Le , Marchi Fabiana , Mauger Stéphane , Michel Gurvan , Potin Philippe , Scornet Delphine , Sotka Erik E. , Weinberger Florian , Cabral de Oliveira Mariana , Guillemin Marie-Laure , Plastino Estela M. , Valero Myriam 
Published: March 2023

ABSTRACTMacroalgal (seaweed) genomic resources are generally lacking as compared to other eukaryotic taxa, and this is particularly true in the red algae (Rhodophyta). Understanding red algal genomes is critical to understanding eukaryotic evolution given that red algal genes are spread across eukaryotic lineages from secondary endosymbiosis and red algae diverged early in the Archaeplastids. The Gracilariales are highly diverse and widely distributed order whose species can serve as ecosystem engineers in intertidal habitats, including several notorious introduced species. The genusGracilariais cultivated worldwide, in part for its production of agar and other bioactive compounds with downstream pharmaceutical and industrial applications. This genus is also emerging as a model for algal evolutionary ecology. Here, we report new whole genome assemblies for two species (G. chilensisandG. gracilis), a draft genome assembly ofG. caudata, and genome annotation of the previously publishedG. vermiculophyllagenome. To facilitate accessibility and comparative analysis, we integrated these data in a newly created web-based portal dedicated to red algal genomics (https://rhodoexplorer.sb-roscoff.fr). These genomes will provide a resource for understanding algal biology and, more broadly, eukaryotic evolution.
Transmembrane Stem Factor Nanodiscs Enhanced Revascularization in a Hind Limb Ischemia Model in Diabetic, Hyperlipidemic Rabbits
Authors: Takematsu Eri , Massidda Miles , Howe Gretchen , Goldman Julia , Felli Patricia , Mei Lei , Callahan Gregory , Sligar Andrew D , Smalling Richard , Baker Aaron B. 
Published: March 2023

AbstractTherapies to revascularize ischemic tissue have long been a goal for the treatment of vascular disease and other disorders. Therapies using stem cell factor (SCF), also known as a c-Kit ligand, had great promise for treating ischemia for myocardial infarct and stroke, however clinical development for SCF was stopped due to toxic side effects including mast cell activation in patients. We recently developed a novel therapy using a transmembrane form of SCF (tmSCF) delivered in lipid nanodiscs. In previous studies, we demonstrated tmSCF nanodiscs were able to induce revascularization of ischemia limbs in mice and did not activate mast cells. To advance this therapeutic towards clinical application, we tested this therapy in an advanced model of hindlimb ischemia in rabbits with hyperlipidemia and diabetes. This model has therapeutic resistance to angiogenic therapies and maintains long term deficits in recovery from ischemic injury. We treated rabbits with local treatment with tmSCF nanodiscs or control solution delivered locally from an alginate gel delivered into the ischemic limb of the rabbits. After eight weeks, we found significantly higher vascularity in the tmSCF nanodisc-treated group in comparison to alginate treated control as quantified through angiography. Histological analysis also showed a significantly higher number of small and large blood vessels in the ischemic muscles of the tmSCF nanodisc treated group. Importantly, we did not observe inflammation or mast cell activation in the rabbits. Overall, this study supports the therapeutic potential of tmSCF nanodiscs for treating peripheral ischemia.
Ultra-flexible endovascular probes for brain recording through micron-scale vasculature
Authors: Zhang Anqi , Mandeville Emiri T. , Xu Lijun , Stary Creed M. , Lo Eng H. , Lieber Charles M. 
Published: March 2023

AbstractImplantable neuroelectronic interfaces have enabled significant advances in both fundamental research and treatment of neurological diseases, yet traditional intracranial depth electrodes require invasive surgery to place and can disrupt the neural networks during implantation. To address these limitations, we have developed an ultra-small and flexible endovascular neural probe that can be implanted into small 100-micron scale blood vessels in the brains of rodents without damaging the brain or vasculature. The structure and mechanical properties of the flexible probes were designed to meet the key constraints for implantation into tortuous blood vessels inaccessible with existing techniques.In vivoelectrophysiology recording of local field potentials and single-unit spikes has been selectively achieved in the cortex and the olfactory bulb. Histology analysis of the tissue interface showed minimal immune response and long-term stability. This platform technology can be readily extended as both research tools and medical devices for the detection and intervention of neurological diseases.
Intramuscular Versus Intravenous SARS-CoV-2 Neutralizing Antibody Sotrovimab for Treatment of COVID-19 (COMET-TAIL): A Randomized Non-inferiority Clinical Trial
Authors: Shapiro Adrienne E. , Sarkis Elias , Acloque Jude , Free Almena , Gonzalez-Rojas Yaneicy , Hussain Rubaba , Juarez Erick , Moya Jaynier , Parikh Naval , Inman David , Cebrik Deborah , Nader Ahmed , Noormohamed Nadia , Wang Qianwen , Skingsley Andrew , Austin Daren , Peppercorn Amanda , Agostini Maria L. , Parra Sergio , Chow Sophia , Mogalian Erik , Pang Phillip S. , Hong David K. , Sager Jennifer E. , Yeh Wendy W. , Alexander Elizabeth L. , Gaffney Leah A. , Kohli Anita 
Published: March 2023

AbstractBackgroundConvenient administration of coronavirus disease 2019 (COVID-19) treatment in community settings is desirable. Sotrovimab is a pan-sarbecovirus dual-action monoclonal antibody formulated for intravenous (IV) or intramuscular (IM) administration for early treatment of mild/moderate COVID-19.MethodsThis phase 3, randomized, multicenter, open-label study tested non-inferiority of IM to IV administration using a 3.5% absolute non-inferiority margin. From June to August 2021, patients aged ≥12 years with COVID-19, not hospitalized or receiving supplemental oxygen, and at high risk for progression were randomized 1:1:1 to a single 500-mg IV sotrovimab infusion or 500-mg or 250-mg IM sotrovimab injection. The primary composite endpoint was progression to all-cause hospitalization for >24 hours for acute management of illness or all-cause death through day 29.ResultsSotrovimab 500 mg IM was non-inferior to 500 mg IV: 10/376 (2.7%) participants in the sotrovimab 500-mg IM group versus 5/378 (1.3%) in the sotrovimab 500-mg IV group met the primary endpoint (absolute adjusted risk difference: 1.06% [95% confidence interval [CI]: −1.15%, 3.26%]). The CI upper limit was lower than the prespecified non-inferiority margin of 3.5%. 250-mg IM group enrollment was discontinued early because a greater proportion of hospitalizations was seen in that group versus the 500-mg groups. Serious adverse events occurred in <1% to 2% of participants across groups. Four participants experienced serious disease related events and died (500 mg IM: 2/393 [<1%]; 250 mg IM: 2/195 [1%]).ConclusionsSotrovimab 500-mg IM injection was well tolerated and non-inferior to IV administration. IM administration could expand outpatient treatment access for COVID-19.RegistrationClinicalTrials.govIdentifier:NCT04913675Key PointsSotrovimab 500-mg IM was non-inferior to sotrovimab 500-mg IV for treatment of mild/moderate COVID-19 in high-risk patients, measured by all-cause hospitalization >24h or death through day 29, and was well-tolerated. Sotrovimab IM should provide easier outpatient access to COVID-19 treatment.
Ultra-Rapid and Specific Gelation of Collagen Molecules for Transparent and Tough Gels by Transition Metal Complexation
Authors: Suezawa Tomoyuki , Sasaki Naoko , Assan Nazgul , Uetake Yuta , Onuma Kunishige , Sakurai Hidehiro , Katayama Ryohei , Inoue Masahiro , Matsusaki Michiya 
Published: March 2023

AbstractCollagen is one of the main components of tumor stromal tissues with a high elastic modulus, but there have been limitations when attempting to fabricate a tough collagen gel with cells like a cancer stroma. Here, we demonstrate the rapid and specific formation of collagen gels with high transparency and high elastic modulus by transition metal complexation within minutes. Transition metal ions such as K2PtCl4exhibited rapid gelation due to the formation of a cross-linked network of the collagen triple-helix by Pt– O and/or Pt–N bonds. Interestingly, type I to IV collagens showed rapid gelation, while other extracellular matrices and DNA did not exhibit this phenomenon, suggesting the importance of intermolecular interaction in a rigid triple-helix structure. Live imaging of colon cancer organoids in three-dimensional culture indicated a collective migration property with modulating high elastic modulus, suggesting activation for metastasis progress. This technology that facilitates deep-live observation and mechanical stiffness adjustment will be useful as a new class of scaffolds.TeaserTransparent collagen gels with tunable mechanical properties allow deep-live observation of cells cultured in a tough environment like our bodies.