bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myelofibrosis-type megakaryocyte dysplasia (MTMD) as a distinct category of BCR::ABL-negative myeloproliferative neoplasms. Challenges and perspectives
Authors: Barosi Giovanni , Rosti Vittorio , Gale Robert Peter 
Published: March 2023

AbstractIn this Perspective, we discuss criteria for defining a new disease entity or variant of a recognized disease or disorder. We do so in the context of the current topography of the BCR::ABL-negative myeloproliferative neoplasms (MPNs) where two new variants are reported: clonal megakaryocyte dysplasia with normal blood values (CMD-NBV) and clonal megakaryocyte dysplasia with isolated thrombocytosis (CMD-IT). The cardinal feature of these variants is bone marrow megakaryocyte hyperplasia and atypia corresponding the WHO histological criteria for primary myelofibrosis (myelofibrosis-type megakaryocyte dysplasia-MTMD). Persons with these new variants have a different disease course and features from others in the MPN domain. In a broader context we suggest myelofibrosis-type megakaryocyte dysplasia defines a spectrum of related MPN variants including CMD-NBV, CMD-IT, pre-fibrotic myelofibrosis and overt myelofibrosis, which differ from polycythemia vera and essential thrombocythemia. Our proposal needs external validation and we stress the need for a consensus definition of the megakaryocyte dysplasia which is the hallmark of these disorders.
Fabrication of practical deformable displays: advances and challenges
Authors: Kim Dong Wook , Kim Seong Won , Lee Gyujeong , Yoon Jangyeol , Kim Sangwoo , Hong Jong-Ho , Jo Sung-Chan , Jeong Unyong 
Published: March 2023

AbstractDisplay form factors such as size and shape have been conventionally determined in consideration of usability and portability. The recent trends requiring wearability and convergence of various smart devices demand innovations in display form factors to realize deformability and large screens. Expandable displays that are foldable, multi-foldable, slidable, or rollable have been commercialized or on the edge of product launches. Beyond such two-dimensional (2D) expansion of displays, efforts have been made to develop three dimensional (3D) free-form displays that can be stretched and crumpled for use in realistic tactile sensation, artificial skin for robots, and on-skin or implantable displays. This review article analyzes the current state of the 2D and 3D deformable displays and discusses the technological challenges to be achieved for industrial commercialization.
Upconversion time-stretch infrared spectroscopy
Authors: Hashimoto Kazuki , Nakamura Takuma , Kageyama Takahiro , Badarla Venkata Ramaiah , Shimada Hiroyuki , Horisaki Ryoich , Ideguchi Takuro 
Published: March 2023

AbstractHigh-speed measurement confronts the extreme speed limit when the signal becomes comparable to the noise level. In the context of broadband mid-infrared spectroscopy, state-of-the-art ultrafast Fourier-transform infrared spectrometers, in particular dual-comb spectrometers, have improved the measurement rate up to a few MSpectra s−1, which is limited by the signal-to-noise ratio. Time-stretch infrared spectroscopy, an emerging ultrafast frequency-swept mid-infrared spectroscopy technique, has shown a record-high rate of 80 MSpectra s−1 with an intrinsically higher signal-to-noise ratio than Fourier-transform spectroscopy by more than the square-root of the number of spectral elements. However, it can measure no more than ~30 spectral elements with a low resolution of several cm−1. Here, we significantly increase the measurable number of spectral elements to more than 1000 by incorporating a nonlinear upconversion process. The one-to-one mapping of a broadband spectrum from the mid-infrared to the near-infrared telecommunication region enables low-loss time-stretching with a single-mode optical fiber and low-noise signal detection with a high-bandwidth photoreceiver. We demonstrate high-resolution mid-infrared spectroscopy of gas-phase methane molecules with a high resolution of 0.017 cm−1. This unprecedentedly high-speed vibrational spectroscopy technique would satisfy various unmet needs in experimental molecular science, e.g., measuring ultrafast dynamics of irreversible phenomena, statistically analyzing a large amount of heterogeneous spectral data, or taking broadband hyperspectral images at a high frame rate.
Evaluation of Cow’s Milk Related Symptom Score [CoMiSS] accuracy in cow’s milk allergy diagnosis
Authors: El-Shafie Ali M. , Omar Zein A. , El Zefzaf Heba M. S. , Basma Elsayedamr M. , Al Sabbagh Nahla M. , Bahbah Wael A. 
Published: March 2023

Abstract
Background
Cow’s Milk-related Symptom Score (CoMiSS) is an awareness tool to recognize cow’s milk allergy (CMA) symptoms in infants. We aimed to assess the best cut-off point of CoMiSS in our country and investigate other parameters suggested to raise the strength of CoMiSS in CMA diagnosis.

Methods
We enrolled 100 infants with CMA-suggestive symptoms with documentation of CoMiSS initially and 4 weeks after cow milk-free diet (CMFD) followed by an open food challenge (OFC) test. Infants with symptom recurrence upon challenge were diagnosed with confirmed CMA.

Results
Initial mean CoMiSS was 15.76 ± 5.29, being higher in the confirmed CMA group (84% of infants). Following CMFD, median CoMiSS significantly reduced to 1.5 in the confirmed CMA group compared to 6.5 in the negative group. Receiver operation characteristic (ROC) curve identified a CoMiSS score of ≥12 as the best cut-off value with 76.19% sensitivity, 62.50% specificity and overall accuracy of 74.00%. Mucoid stool, bloody stool and faltering growth were reported in 80, 41 and 52% of confirmed CMA infants, respectively, with considerable improvement following CMFD.

Conclusions
Our study revealed a CoMiSS score of ≥12 to be the best cut-off point. However, CoMiSS cannot be used alone for accurate diagnosis of CMA.

Impact

CoMiSS ≥12 can predict a positive response to CMFD; nevertheless, CoMiSS is a good awareness tool and cannot be regarded as a stand-alone CMA diagnostic test.

CoMiSS reduction following CMFD was predictive of a reaction to OFC to diagnose CMA as well as for monitoring symptom improvement.

Symptoms commonly associated with CMA as mucoid stool, bloody stool, marked abdominal distention not responding to medical treatment and faltering growth, in addition to their improvements in response to CMA are suggested parameters to be added to CoMiSS to improve its accuracy.
Safety, tolerability, and pharmacokinetics of Aurora kinase B inhibitor AZD2811: a phase 1 dose-finding study in patients with advanced solid tumours
Authors: Johnson Melissa L. , Wang Judy S. , Falchook Gerald , Greenlees Carol , Jones Suzanne , Strickland Donald , Fabbri Giulia , Kennedy Caroline , Elizabeth Pease J. , Sainsbury Liz , MacDonald Alexander , Schalkwijk Stein , Szekeres Philip , Cosaert Jan , Burris Howard 
Published: March 2023

Abstract
Background
AZD2811 is a potent, selective Aurora kinase B inhibitor. We report the dose-escalation phase of a first-in-human study assessing nanoparticle-encapsulated AZD2811 in advanced solid tumours.

Methods
AZD2811 was administered in 12 dose-escalation cohorts (2-h intravenous infusion; 15‒600 mg; 21-/28-day cycles) with granulocyte colony-stimulating factor (G-CSF) at higher doses. The primary objective was determining safety and maximum tolerated/recommended phase 2 dose (RP2D).

Results
Fifty-one patients received AZD2811. Drug exposure was sustained for several days post-dose. The most common AZD2811-related adverse events (AEs) were fatigue (27.3%) at ≤200 mg/cycle and neutropenia (37.9%) at ≥400 mg/cycle. Five patients had dose-limiting toxicities: grade (G)4 decreased neutrophil count (n = 1, 200 mg; Days 1, 4; 28-day cycle); G4 decreased neutrophil count and G3 stomatitis (n = 1 each, both 400 mg; Day 1; 21-day cycle); G3 febrile neutropenia and G3 fatigue (n = 1 each, both 600 mg; Day 1; 21-day cycle +G-CSF). RP2D was 500 mg; Day 1; 21-day cycle with G-CSF on Day 8. Neutropenia/neutrophil count decrease were on-target AEs. Best overall responses were partial response (n = 1, 2.0%) and stable disease (n = 23, 45.1%).

Conclusions
At RP2D, AZD2811 was tolerable with G-CSF support. Neutropenia was a pharmacodynamic biomarker.

Clinical trial registration
NCT02579226.
CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy
Authors: Bai Xue , Guo Ze-Qin , Zhang Yan-Pei , Fan Zhen-zhen , Liu Li-Juan , Liu Li , Long Li-Li , Ma Si-Cong , Wang Jian , Fang Yuan , Tang Xin-Ran , Zeng Yu-Jie , Pan Xinghua , Wu De-Hua , Dong Zhong-Yi 
Published: March 2023

AbstractLiver kinase B1 (LKB1) mutation is prevalent and a driver of resistance to immune checkpoint blockade (ICB) therapy for lung adenocarcinoma. Here leveraging single cell RNA sequencing data, we demonstrate that trafficking and adhesion process of activated T cells are defected in genetically engineered Kras-driven mouse model with Lkb1 conditional knockout. LKB1 mutant cancer cells result in marked suppression of intercellular adhesion molecule-1 (ICAM1). Ectopic expression of Icam1 in Lkb1-deficient tumor increases homing and activation of adoptively transferred SIINFEKL-specific CD8+ T cells, reactivates tumor-effector cell interactions and re-sensitises tumors to ICB. Further discovery proves that CDK4/6 inhibitors upregulate ICAM1 transcription by inhibiting phosphorylation of retinoblastoma protein RB in LKB1 deficient cancer cells. Finally, a tailored combination strategy using CDK4/6 inhibitors and anti-PD-1 antibodies promotes ICAM1-triggered immune response in multiple Lkb1-deficient murine models. Our findings renovate that ICAM1 on tumor cells orchestrates anti-tumor immune response, especially for adaptive immunity.
Robust flexural performance and fracture behavior of TiO2 decorated densified bamboo as sustainable structural materials
Authors: Ba Ziyu , Luo Hongyun , Guan Juan , Luo Jun , Gao Jiajia , Wu Sujun , Ritchie Robert O. 
Published: March 2023

AbstractHigh-performance, fast-growing natural materials with sustainable and functional features currently arouse significant attention. Here, facile processing, involving delignification, in situ hydrothermal synthesis of TiO2 and pressure densification, is employed to transform natural bamboo into a high-performance structural material. The resulting TiO2-decorated densified bamboo exhibits high flexural strength and elastic stiffness, with both properties more than double that of natural bamboo. Real-time acoustic emission reveals the key role of the TiO2 nanoparticles in enhancing the flexural properties. The introduction of nanoscale TiO2 is found to markedly increase the degree of oxidation and the formation of hydrogen bonds in bamboo materials, leading to extensive interfacial failure between the microfibers, a micro-fibrillation process that results in substantial energy consumption and high fracture resistance. This work furthers the strategy of the synthetic reinforcement of fast-growing natural materials, which could lead to the expanded applications of sustainable materials for high-performance structural applications.
Engineered hypermutation adapts cyanobacterial photosynthesis to combined high light and high temperature stress
Authors: Sun Huili , Luan Guodong , Ma Yifan , Lou Wenjing , Chen Rongze , Feng Dandan , Zhang Shanshan , Sun Jiahui , Lu Xuefeng 
Published: March 2023

AbstractPhotosynthesis can be impaired by combined high light and high temperature (HLHT) stress. Obtaining HLHT tolerant photoautotrophs is laborious and time-consuming, and in most cases the underlying molecular mechanisms remain unclear. Here, we increase the mutation rates of cyanobacterium Synechococcus elongatus PCC 7942 by three orders of magnitude through combinatory perturbations of the genetic fidelity machinery and cultivation environment. Utilizing the hypermutation system, we isolate Synechococcus mutants with improved HLHT tolerance and identify genome mutations contributing to the adaptation process. A specific mutation located in the upstream non-coding region of the gene encoding a shikimate kinase results in enhanced expression of this gene. Overexpression of the shikimate kinase encoding gene in both Synechococcus and Synechocystis leads to improved HLHT tolerance. Transcriptome analysis indicates that the mutation remodels the photosynthetic chain and metabolism network in Synechococcus. Thus, mutations identified by the hypermutation system are useful for engineering cyanobacteria with improved HLHT tolerance.
DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation
Authors: Xu Xiaohua , Chang Chou-Wei , Li Min , Omabe Kenneth , Le Nhung , Chen Yi-Hsuan , Liang Feng , Liu Yilun 
Published: March 2023

AbstractDeletion of the conserved C-terminus of the Rothmund-Thomson syndrome helicase RECQ4 is highly tumorigenic. However, while the RECQ4 N-terminus is known to facilitate DNA replication initiation, the function of its C-terminus remains unclear. Using an unbiased proteomic approach, we identify an interaction between the RECQ4 N-terminus and the anaphase-promoting complex/cyclosome (APC/C) on human chromatin. We further show that this interaction stabilizes APC/C co-activator CDH1 and enhances APC/C-dependent degradation of the replication inhibitor Geminin, allowing replication factors to accumulate on chromatin. In contrast, the function is blocked by the RECQ4 C-terminus, which binds to protein inhibitors of APC/C. A cancer-prone, C-terminal-deleted RECQ4 mutation increases origin firing frequency, accelerates G1/S transition, and supports abnormally high DNA content. Our study reveals a role of the human RECQ4 C-terminus in antagonizing its N-terminus, thereby suppressing replication initiation, and this suppression is impaired by oncogenic mutations.
Exploring and machine learning structural instabilities in 2D materials
Authors: Manti Simone , Svendsen Mark Kamper , Knøsgaard Nikolaj R. , Lyngby Peder M. , Thygesen Kristian S. 
Published: March 2023

AbstractWe address the problem of predicting the zero-temperature dynamical stability (DS) of a periodic crystal without computing its full phonon band structure. Here we report the evidence that DS can be inferred with good reliability from the phonon frequencies at the center and boundary of the Brillouin zone (BZ). This analysis represents a validation of the DS test employed by the Computational 2D Materials Database (C2DB). For 137 dynamically unstable 2D crystals, we displace the atoms along an unstable mode and relax the structure. This procedure yields a dynamically stable crystal in 49 cases. The elementary properties of these new structures are characterized using the C2DB workflow, and it is found that their properties can differ significantly from those of the original unstable crystals, e.g., band gaps are opened by 0.3 eV on average. All the crystal structures and properties are available in the C2DB. Finally, we train a classification model on the DS data for 3295 2D materials in the C2DB using a representation encoding the electronic structure of the crystal. We obtain an excellent receiver operating characteristic (ROC) curve with an area under the curve (AUC) of 0.90, showing that the classification model can drastically reduce computational efforts in high-throughput studies.
Learning the right channel in multimodal imaging: automated experiment in piezoresponse force microscopy
Authors: Liu Yongtao , Vasudevan Rama K. , Kelley Kyle P. , Funakubo Hiroshi , Ziatdinov Maxim , Kalinin Sergei V. 
Published: March 2023

AbstractWe report the development and experimental implementation of the automated experiment workflows for the identification of the best predictive channel for a phenomenon of interest in spectroscopic measurements. The approach is based on the combination of ensembled deep kernel learning for probabilistic predictions and a basic reinforcement learning policy for channel selection. It allows the identification of which of the available observational channels, sampled sequentially, are most predictive of selected behaviors, and hence have the strongest correlations. We implement this approach for multimodal imaging in piezoresponse force microscopy (PFM), with the behaviors of interest manifesting in piezoresponse spectroscopy. We illustrate the best predictive channel for polarization-voltage hysteresis loop and frequency-voltage hysteresis loop areas is amplitude in the model samples. The same workflow and code are applicable for any multimodal imaging and local characterization methods.
A comparative blind study between skin biopsy and seed amplification assay to disclose pathological α-synuclein in RBD
Authors: Liguori R. , Donadio V. , Wang Z. , Incensi A. , Rizzo G. , Antelmi E. , Biscarini F. , Pizza F. , Zou Wq , Plazzi G. 
Published: March 2023

AbstractTo compare the diagnostic accuracy of the immunofluorescence (IF) technique and aSyn-seed amplification assay (aSyn-SAA) of skin and cerebrospinal fluid (CSF) in disclosing pathological α-syn in idiopathic idiopathic REM sleep behavior disorder (iRBD) as early phase of a synucleinopathy. We prospectively recruited 41 patients with iRBD and 40 matched clinical controls including RBD associated with type 1 Narcolepsy (RBD-NT1, 21 patients), iatrogenic causes (2 pt) or OSAS (6 pt) and 11 patients with peripheral neuropathies. IF from samples taken by skin biopsy and aSyn-SAA from skin and CSF samples were analysed blinded to the clinical diagnosis. IF showed a good diagnostic accuracy (89%) that was lower in the case of skin and CSF-based aSyn-SAA (70% and 69%, respectively) because of a lower sensitivity and specificity. However, IF showed a significant agreement with CSF aSyn-SAA. In conclusion, our data may favor the use of skin biopsy and aSyn-SAA as diagnostic tools for a synucleinopathy in iRBD.
Application of an algorithm to analyze patterns of intermittent oral corticosteroid use in asthma
Authors: Haughney John , Tran Trung N. , Heatley Heath , Bourdin Arnaud , Menzies-Gow Andrew , Jackson David J. , Maslova Ekaterina , Chapaneri Jatin , Skinner Derek , Carter Victoria , Chan Jeffrey Shi Kai , Price David 
Published: March 2023

AbstractAn algorithm to describe patterns of intermittent oral corticosteroid use in the UK (n = 476,167) found that one-third of patients receiving intermittent oral corticosteroids for asthma only had short gaps (<90 days) between oral corticosteroid prescriptions sometime during follow-up. The increasing frequency pattern was more likely in patients with greater asthma severity and with more short-acting β2-agonist use at baseline. Our approach may provide a clinically relevant representation of intermittent oral corticosteroid use in asthma.
Parallel tomography of quantum non-demolition measurements in multi-qubit devices
Authors: Pereira L. , García-Ripoll J. J. , Ramos T. 
Published: March 2023

AbstractAn efficient characterization of QND measurements is an important ingredient toward certifying and improving the performance and scalability of quantum processors. In this work, we introduce a parallel tomography of QND measurements that addresses single- and two-qubit readout on a multi-qubit quantum processor. We provide an experimental demonstration of the tomographic protocol on a 7-qubit IBM-Q device, characterizing the quality of conventional qubit readout as well as generalized measurements such as parity or measurement-and-reset schemes. Our protocol reconstructs the Choi matrices of the measurement processes, extracts relevant quantifiers—fidelity, QNDness, destructiveness—and identifies sources of errors that limit the performance of the device for repeated QND measurements. We also show how to quantify measurement crosstalk and use it to certify the quality of simultaneous readout on multiple qubits.
Evaluation of two terpene-derived polymers as consolidants for archaeological wood
Authors: Cutajar Michelle , Braovac Susan , Stockman Robert A. , Howdle Steven M. , Harding Stephen E. 
Published: March 2023

AbstractThe evaluation of two terpene-derived polymers, termed TPA6 and TPA7, as possible consolidants for archaeological wood was carried out. The overall objective of this work was to expand the non-aqueous treatment toolkit which is available for the conservation of the highly degraded Oseberg collection. The wood artefacts which were found on the Oseberg ship were treated with alum in the early twentieth century, leading to the formation of sulfuric acid and to the precarious state that they are in today. Some of these artefacts cannot be treated with conventional aqueous consolidants, like polyethylene glycol, due to their highly degraded and/or reconstructed nature. This study sought to examine the level of penetration of the polymers in archaeological wood and to evaluate their consolidative effect. Both TPA6 and TPA7 were soluble in isopropanol and had a Mw of 3.9 and 4.2 kDa respectively. A number of archaeological wood specimens were immersed in solutions of these polymers. Their penetration and effects were evaluated using weight and dimensional change, colour change, infrared spectroscopy, scanning electron microscopy and hardness tests. Both polymers successfully penetrated the wood specimens, with a higher concentration found on the surface versus the core. Additionally, both polymers appeared to increase the hardness of the specimen surfaces. Increasing the polymer concentration and soaking time in future investigations could potentially facilitate the penetration to the wood cores.
Local environment drives rapid shifts in composition and phylogenetic clustering of seagrass microbiomes
Authors: Kardish Melissa R. , Stachowicz John. J. 
Published: March 2023

AbstractPlant microbiomes depend on environmental conditions, stochasticity, host species, and genotype identity. Eelgrass (Zostera marina)is a unique system for plant–microbe interactions as a marine angiosperm growing in a physiologically-challenging environment with anoxic sediment, periodic exposure to air at low tide, and fluctuations in water clarity and flow. We tested the influence of host origin versus environment on eelgrass microbiome composition by transplanting 768 plants among four sites within Bodega Harbor, CA. Over three months following transplantation, we sampled microbial communities monthly on leaves and roots and sequenced the V4–V5 region of the 16S rRNA gene to assess community composition. The main driver of leaf and root microbiome composition was destination site; more modest effects of host origin site did not last longer than one month. Community phylogenetic analyses suggested that environmental filtering structures these communities, but the strength and nature of this filtering varies among sites and over time and roots and leaves show opposing gradients in clustering along a temperature gradient. We demonstrate that local environmental differences create rapid shifts in associated microbial community composition with potential functional implications for rapid host acclimation under shifting environmental conditions.