bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathological Features, Immune Infiltration Landscape and Involved Signaling Pathways of the desmogleins family in Pancreatic Adenocarcinoma
Authors: Lin Zhenyu , Du Yongxing , Duan YunJie , Zhang Wei , Huang Zhangkan , Yin Xin , Ma Teng , Xie Yunliang , Che Xu 
Published: May 2023

Abstract
Background
A growing body of evidence suggests that the DSG family plays a key role in tumorigenesis and progression; however, the function of DSG family members in PAAD remains unclear.
Methods
Comprehensive bioinformatics analysis was performed to investigate the clinicopathological characteristics, prognostic value, imnological features, and functional mechanisms of DSG family members in PAAD, using UALCAN, the HPA, Kaplan–Meier Plotter, cBioPortal, TISIDB, LinkedOmics, STRING and GSCALite Database.
Results
The expression of DSG family members was significantly higher in PAAD tissues compared with paraneoplastic or normal tissues, and their copy number variation was significantly associated with poorer clinicopathological characteristics and prognosis in PAAD patients. Furthermore, the roles of DSG family members in immune regulation are diverse and complex. Mechanistically, TP53 mutations are significantly associated with promoter methylation and the expression of DSG family members, and EGFR may be key to the role of DSG family members in PAAD. DSG family members activate several oncogenic pathways, including EMT, PI3K/AKT, and RAS/MAPK signaling pathway. In addition, we found that the expression of DSG family members was significantly correlated with sensitivity to multiple conventional chemotherapeutic agents and novel targeted drugs.
Conclusions
DSG family members play an oncogenic role in the development of PAAD and may serve as novel biomarkers or therapeutic targets.
Tau Accumulation in Degradative Organelles is Associated to Lysosomal Stress
Authors: Piovesana Ester , Magrin Claudia , Ciccaldo Matteo , Sola Martina , Bellotto Manolo , Molinari Maurizio , Papin Stéphanie , Paganetti Paolo 
Published: May 2023

Abstract
Neurodegenerative disorders are characterized by the brain deposition of insoluble amyloidogenic proteins, such as α-synuclein or Tau, and the concomitant deterioration of cell functions such as the autophagy-lysosomal pathway (ALP). The ALP is involved in the degradation of intracellular macromolecules including protein aggregates. ALP dysfunction due to inherited defects in lysosomal or non-lysosomal proteins causes a group of diseases called lysosomal storage disorders (LSD) because of abnormal accumulation of lysosomal degradation substrates. Supporting the contribution of ALP defects in neurodegenerative diseases, deposition of amyloidogenic proteins occurs in LSD. Moreover, heterozygous mutations of several ALP genes represent risk factors for Parkinson’s disease. The reciprocal contribution of α-synuclein accumulation and lysosomal dysfunction have been extensively studied. However, whether this adverse crosstalk also embraces Tau pathology needs more investigation. Here, we show in human primary fibroblasts that Tau seeds isolated from the brain of Alzheimer’s disease induce Tau accumulation in acidic degradative organelles and lysosomal stress. Furthermore, inhibition of glucocerebrosidase, a lysosomal enzyme mutated in Gaucher’s disease and a main risk for Parkinson’s disease, causes lysosomal dysfunction in primary fibroblasts and contributes to the accumulation of Tau. Considering the presence of Tau lesions in Parkinson’s disease as well as in multiple neurodegenerative disorders including Alzheimer’s disease, our data call for further studies on strategies to alleviate ALP dysfunction as new therapeutic opportunity for neurodegenerative diseases and LSD.
Effects of atrazine and S-metolachlor on stream periphyton taxonomic and fatty acid composition
Authors: Malbezin Laura , Morin Soizic , Lavoie Isabelle 
Published: May 2023

Abstract
Extensive pesticide use for agriculture diffusely pollutes aquatic ecosystems through leaching and runoff events and has the potential to negatively affect non-target organisms. Atrazine and S-metolachlor are two widely used herbicides often detected in high concentrations in rivers that drain nearby agricultural lands. To determine the effects of these two herbicides on river ecosystems, we conducted a 14-day laboratory experiment to expose river periphyton to a wide range of atrazine and S-metolachlor concentrations. The response of periphyton was evaluated using ecologically relevant endpoints including chlorophyll a fluorescence and fatty acids composition. Results showed that atrazine disrupted photoautotroph biomass measured by chlorophyll a fluorescence. Both herbicides caused dissimilarities in fatty acid profiles between control and high exposure concentrations, but S-metolachlor had a stronger effect than atrazine on the observe increase or reduction in saturated fatty acids (SFAs) and very long chain fatty acids (VLCFAs). Our study demonstrates that two commonly used herbicides, atrazine and S-metolachlor, can negatively affect the composition and fatty acid profiles of stream periphyton, thereby altering the nutritional quality of this resource for primary consumers.
Synthesizes, characterization, molecular modelling studies and bioactivity of a novel bicyclic compound of δ-lactam with oxazepine ring containing-sulphur substitute using an economic method
Authors: Muslim Rasim Farraj , Guma Manaf A. , Abedulrahman May F. , Ahmad Iqrar , Patel Harun , Rabeea Muwafaq A 
Published: May 2023

Abstract
Penicillin resistance is a commonly present and controversial matter due to the misuses by people for various reasons. However, little studies have been examined the bioactivity of the 5 and 6 membered rings. In this study, we aimed to synthesize a new compound contain 5- membered ring following a short and low cost method and combined it with oxazepine ring via Schiff bases to produce a bicyclic molecule (Lactozepine). In vitro examinations were implemented to assess the bioactivity of the prepared compound such as anti-bacterial, anti-fungal and anti-oxidant which has shown a wide zone of inhibition of lactozepine against Streptococcus pneumoniae but no inhibition was shown against Kelbesilla pneumoniae and Staphylococcus aureus except at a high concentration similar to the result of the anti-fungal assessment. Furthermore, lactozepine showed worthy anti-oxidant activity against free radical formation. The molecular modelling and docking assessment showed availability of lactozepine to bind to bacterial proteins and inhibit their growth with lowest free energy for the greatest and strong binding affinity with the PDB crystal structures 1VQQ, 2WAE, 1PYY and 1IYS were between − 6.5 and − 7.9 kcal/mol. Moreover, the molecular MD dynamic simulation showed that RMSF (root mean square fluctuation) for the assessed protein's amino acids remained consistent and tightly bound to lactozepine in the dynamic state. The novel compound of lactozepine having δ-lactam rings attached to oxazepine showed a bioactivity that are hopeful for in vivo studies in the future.
Characterization of Volatile Compounds in Rose Hydrosol Using Gas Chromatography-Mass Spectrometry: Sensory Evaluation and Antioxidant activities
Authors: Wan Yilai , Li Yazhuan , Liu Lingzhen , Li Fuhe , Wang Na , Zhang Tingting , Wei Changqing , Liu Wenyu 
Published: May 2023

Abstract
In this study, Yunnan Dark Red Rose petals were used as experimental materials, and rose hydrosol was extracted by steam distillation. Under the optimal extraction parameters, whether stamens were added or not was also taken into account. Four groups of control rose hydrosol samples were set up for sensory evaluation, and the sample with strong aroma was selected. The optimal extraction conditions were as follows: no stamens were added, the ratio of fixed material to liquid was 1:3, distillation time was 3 h, and the concentration of sodium chloride was 3%. The volatile substances in the rose hydrosol were analyzed and evaluated by gas chromatography-mass spectrometry (GC-MS). The results showed that the main aroma substances in the pure rose dew were linalool, citronellol, β-phenylethanol, benzyl alcohol, eugenol methyl ether, eugenol, perilla alcohol, etc. These substances had great influence on the overall aroma of rose hydrosol, which could better reflect the aroma characteristics of roses. The antioxidant activity was analyzed and evaluated by the scavenging rates of rose hydrosol on DPPH, ·OH and superoxide anion (·O2-) respectively. The scavenging rates of DPPH, ·OH and ·O2- in rose hydrosol at 100 mg/mL were 26.13%, 55.56% and 10.76%, and the contents of polyphenols and flavonoids were 14.9 ± 2.5 gallic acid equivalent (GAE) mg/g and 19.3 ± 1.3 rutin equivalent (RE) mg/g respectively, which indicated that the extracted rose hydrosol had good antioxidation.
Kinematics and activation of hamstring muscles during the initial acceleration phase of sprinting in soccer players: A biomechanical investigation
Authors: Le Thiet Van , Kikuchi Yudai , Takabayashi Tomoya , Kikumoto Takanori , Kubo Masayoshi 
Published: May 2023

Abstract
Purpose Sprinting is the most common activity associated with hamstring strain injuries in soccer. However, the potential mechanisms for hamstring strain injuries during the initial acceleration phase of sprinting are unclear. Therefore, this study aimed to characterize the kinematics and activation of the hamstring muscles in soccer players to define the potential mechanism of hamstring strain injury.Methods Ten male soccer players with no lower-limb injuries in the past year were recruited for this study. Each participant was instructed to perform a minimum of three successful 15 m sprints. Hamstring kinematics and activations were estimated by employing musculoskeletal modeling and wireless electromyography to define potential injury mechanisms.Results During the initial acceleration phase, the hamstring muscles undergo great strain and high activation levels during the late swing and early stance phases. The hamstring muscles experienced great strain and overload during the late swing and foot strike of the running gait cycle. Additionally, the biceps femoris long head was significantly more stretched than the semimembranosus and semitendinosus (p < 0.0001, p < 0.05).Conclusion Based on these results, hamstring injuries are likely to occur during the late-swing and early stance of the running gait cycle, with the biceps femoris long head being more susceptible to injuries than other hamstring muscles.
Evaluation of soil water losses under irrigation saving techniques in a semi-arid region in Tunisia
Authors: Ammar Haroun Ben , Fridhi Rebh , Kanzari Sabri , Nouna Béchir Ben 
Published: May 2023

Abstract
In arid and semi-arid regions, managing agricultural water for irrigation is essential to cope with water scarcity and maximize crop yields. In this study, an experiment was conducted on a potato crop in the Manouba region (lower valley of Medjerda, Tunisia). The experimental protocol consisted of four water treatments utilizing water-saving irrigation techniques: FI (Full Irrigation 100%): irrigation with 100% of crop water requirements. DI (Irrigation 75%): deficit irrigation with the application of 75% of crop water requirements. PRDRight (Irrigation 50% on the right side): Irrigation by partial root drying. PRDLeft (Irrigation 50% on the left side): Irrigation by partial root drying. Simulation of soil water profiles was carried out by the Hydrus-1D model. The soil hydraulic properties were calibrated according to the experimental conditions using an inverse modeling technique. According to the obtained results, simulated soil water profiles were close to those measured. Indeed, the calculated NRMSE values are low, indicating the reliability of Hydrus-1D as a decision support tool to optimize water irrigation management. These results were then used to investigate the effects of a 2 °C temperature increase on soil water loss, and it was determined that the impact was insignificant.
Berberine Decreases Thrombosis Potential Induced by a High-choline
Diet by Inhibiting CutC Enzyme
Authors: Qu Hua , Zhang Ying , Shi Jun-he , Zhao Yi-han , Gao Jie , Gao Zhu-ye , Shi Da-zhuo 
Published: May 2023

Introduction:
Gut microbes influence thrombosis potential by generating
trimethylamine N-oxide (TMAO). However, whether the antithrombotic effect of berberine
is associated with TMAO generation remains unclear.

Objective:
The present study was designed to explore whether berberine decreases the
TMAO-induced thrombosis potential and the possible mechanism underneath it.

Methods:
C57BL/6J female mice under a high-choline diet or standard diet were treated
with/without berberine for 6 weeks. The TMAO level, carotid artery occlusion time following
FeCl3 injury and platelet responsiveness were measured. The binding of berberine
to the CutC enzyme was analysed with molecular docking, and molecular dynamics simulations
were verified with enzyme activity assays.

Result:
The results showed that berberine increased the carotid artery occlusion time following
FeCl3 injury and decreased the platelet hyperresponsiveness induced by a high--
choline diet, both offset by intraperitoneal injection of TMAO. The effect of berberine on
thrombosis potential was associated with decreasing the generation of TMAO by inhibiting
the CutC enzyme.

Conclusion:
Targeting TMAO generation with berberine might be a promising therapy
for ischaemic cardiac-cerebral vascular diseases.
Association of Blood Lipid Profile Components with White Matter Hyperintensity
Burden in Cerebral Small Vessel Disease
Authors: Li Shanshan , Wang Yunchao , Yu Lulu , Gao Yuan , Yang Yinghao , Zhu Hanghang , An Lu , Yuan Wenxin , Wu Jinghao , Zong Ce , Xu Yuming , Li Yusheng 
Published: May 2023

Background:
Cerebral small vessel disease (CSVD) refers to a common cerebrovascular
disease and white matter hyperintensities (WMHs) constitute a typical feature of CSVD. However,
there has not been a large number of studies investigating the relationship between lipid profile
components and WMHs.

Methods:
Altogether, 1019 patients with CSVD were enrolled at the First Affiliated Hospital of
Zhengzhou University between April 2016 to December 2021. Baseline data were collected for all
patients, including demographic characteristics and clinical data. WMH volumes were evaluated
by two experienced neurologists using the MRIcro software. Multivariate regression analysis was
used to investigate the relationship among the severity of WMHs, blood lipids and common risk
factors.

Results:
Altogether, 1019 patients with CSVD were enrolled, including 255 in the severe WMH
group and 764 in the mild WMH group. After including age, sex and blood lipids to construct a
multivariate logistic regression equation, we observed that the severity of WMHs was independently
predicted by low-density lipoprotein (LDL), homocysteine level and history of cerebral
infarction.

Conclusion:
We used WMH volume, a highly accurate measure, to assess its relationship with
lipid profiles. The WMH volume increased with a decrease in LDL. This relationship was more
significant, especially among the subgroups of patients aged <70 years and men. Patients with cerebral
infarction and higher homocysteine levels were more likely to have higher WMH volumes.
Our study has provided a reference for clinical diagnosis and therapy, especially for discussing the
role of blood lipid profiles in the pathophysiology of CSVD.
Cogstim: A Shared Decision-making Model to Support Older Adults’
Brain Health
Authors: Ownby Raymond L. , Waldrop Drenna 
Published: May 2023

Abstract:
The lack of effective treatments for cognitive decline in older adults has led to an interest
in the possibility that lifestyle interventions can help to prevent changes in mental functioning and
reduce the risk for dementia. Multiple lifestyle factors have been related to risk for decline, and multicomponent
intervention studies suggest that changing older adults’ behaviors can have a positive
impact on their cognition. How to translate these findings into a practical model for clinical use with
older adults, however, is not clear. In this Commentary, we propose a shared decision-making model
to support clinicians’ efforts to promote brain health in older persons. The model organizes risk and
protective factors into three broad groups based on their mechanism of action and provides older persons
with basic information to allow them to make evidence- and preference-based choices in choosing
goals for effective brain health programs. A final component includes basic instruction in behavior
change strategies such as goal setting, self-monitoring, and problem-solving. The implementation
of the model will support older persons’ efforts to develop a personally relevant and effective brainhealthy
lifestyle that may help to reduce their risk for cognitive decline.
MIR-29A-3P, MIR-29C-3P, MIR-146B-5P AND MIR-150-5P, Their
Target Genes and lncrnas in HIV Infection: A Bioinformatic Study
Authors: Tumolo Maria Rosaria , Scoditti Egeria , Guarino Roberto , Grassi Tiziana , Bagordo Francesco , Sabina Saverio 
Published: May 2023

Introduction:
Increasing evidence suggests that microRNAs (miRNAs) and long noncoding
RNAs (lncRNAs) have emerged as attractive targets in viral infections, including Human
immunodeficiency virus (HIV).

Objective:
To deepen the understanding of the molecular mechanisms that lead to HIV and provide
potential targets for the future development of molecular therapies for its treatment.

Methods:
Four miRNAs were selected as candidates based on a previous systematic review. A
combination of bioinformatic analyses was performed to identify their target genes, lncRNAs and
biological processes that regulate them.

Results:
In the constructed miRNA–mRNA network, 193 gene targets are identified. These miRNAs
potentially control genes from several important processes, including signal transduction and
cancer. LncRNA-XIST, lncRNA-NEAT1 and lncRNA-HCG18 interact with all four miRNAs.

Conclusions:
This preliminary result forms the basis for improving reliability in future studies to fully
understand the role these molecules and their interactions play in HIV.
PEMBELAJARAN BIOLOGI SECARA DARING DAN PENGARUHNYA TERHADAP KEMANDIRIAN BELAJAR DAN LITERASI DIGITAL SISWA
Authors: Prammudya Reynaldi Desta , Subiantoro Agung Wijaya 
Published: May 2023

Penelitian bertujuan untuk: (1) mengetahui pelaksanaan pembelajaran daring di kelas XI IPA SMA Negeri 12 Banyuasin III dan (2) mengathui gambaran tingkat kemandirian belajar serta (3) mengetahui gambaran komptensi literasi digital siswa dalam pembelajaran daring selama Pandemi Covid-19. Penelitian ini termasuk penelitian deskriptif dengan pendekatan studi kasus. Populasi penelitian yaitu peserta didik SMA Negeri 12 Banyuasin III dengan sampel penelitian yaitu peserta didik kelas XI IPA di SMA Negeri 12 Banyuasin III berjumlah 83 peserta didik yang diperoleh melalui teknik pusrposive sampling. Teknik pengumpulan data menggunakan angket, pedoman wawancara dan soal tes literasi digital. Teknik analisis data menggunakan statistik deskriptif. Hasil penelitian menunjukan bahwa (1) pelaksanaan pembelajaran selama pandemi di SMA Negeri 12 Banyuasin III dilakukan dengan bantuan aplikasi pembelajaran Google Classroom, Google meet/Zoom Meeting, Quizizz, Youtube. (2) gambaran tingkat kemandirian belajar siswa Selama pembelajaran daring, menunjukan Siswa memiliki tingkat kemandirian belajar yang kuat. Berdasarkan dari hasil analisis persentase setiap indikator kemandirian belajar siswa, rata-rata persentase keseluruhan indikator sebesar 78,2 %. (3) komptensi literasi digital Siswa berdasarkan hasil analisis angket, persentase setiap indikator literasi digital siswa sebesar 71,5 %. Hasil uji tes soal literasi digital sebesar 76 %. Hal ini menunjukkan bahwa indikator literasi digital siswa berada pada kategori tinggi.
Pengembangan Media Pembelajaran Berbasis Aplikasi Android Pada Materi Sistem Pernapasan Kelas XI SMA
Authors: Miranda Delta , Wibowo Yuni 
Published: May 2023

Penelitian ini merupakan penelitian untuk mengembangkan media pembelajaran berbasis aplikasi android pada materi sistem pernapasan kelas XI SMA. Hal ini didasari dengan kegiatan pembelajaran di SMAN 1 Wates menggunakan buku paket. Seluruh siswa memiliki smartphone, namun belum terdapat aplikasi yang digunakan sebagai media pembelajaran. Tujuan penelitian ini adalah: 1) Untuk mengembangkan media pembelajaran berbasis aplikasi android pada materi sistem pernapasan kelas XI SMA; 2) Untuk mengetahui validitas media pembelajaran berbasis aplikasi android pada materi sistem pernapasan menurut ahli media, ahli materi dan guru biologi; 3) Untuk mengetahui respon siswa terhadap prototipe media pembelajaran berbasis aplikasi android pada materi sistem pernapasan. Jenis penelitian ini adalah penelitian pengembangan Research and Development (RnD) model ADDIE. Penelitian ini hanya dilakukan sampai tahap development, karena keterbatasan biaya dan perbedaan waktu implementasi materi sistem pernapasan dengan waktu penelitian. Instrumen pengumpulan data menggunakan lembar validasi ahli, lembar penilaian guru dan angket uji keterbacaan siswa. Penelitian ini menunjukkan hasil validasi ahli media sebesar 98,07% atau sangat layak digunakan. Hasil validasi ahli materi memperoleh nilai sebesar 84,25 % atau layak digunakan. Hasil penilaian oleh guru biologi menunjukkan media pembelajaran ini sangat layak sebagai media pembelajaran biologi, siswa juga memberikan respon sangat baik terhadap prototipe media pembelajaran ini. Kata Kunci : pengembangan, media pembelajaran, aplikasi android, sistem pernapasan, validitas.
PENGARUH PEMBELAJARAN BIOLOGI BERBASIS ADI TOPIK KEANEKARAGAMAN HAYATI UNTUK MENINGKATKAN KEMAMPUAN ARGUMENTASI SISWA
Authors: Putri Wina Afifah , Paidi Paidi 
Published: May 2023

Penelitian ini bertujuan untuk mengetahui pengaruh pembelajaran biologi berbasis Argument-Driven Inquiry (ADI) topik keanekaragaman hayati dalam peningkatan kemampuan argumentasi siswa.Penelitian ini termasuk dalam jenis penelitian kuasi eksperimen dengan menggunakan pretest-posttest nonequivalent control group design.Variabel bebas pada penelitian ini adalah penggunaan model pembelajaran Argument-Driven Inquiry (ADI). Populasi pada penelitian ini adalah seluruh siswa kelas X SMA N 1 Sanden, Bantul. Sampel pada penelitian ini menggunakan dua kelas, yaitu kelas X5 yang terpilih sebagai kelas eksperimen dan kelas X6 yang terpilih sebagai kelas kontrol. Pengumpulan data kemampuan argumentasi siswa menggunakan tes dalam bentuk soal uraian. Teknik analisis data yang digunakan adalah Uji Mann-Whitney, Uji Jarak Bertanda Wilcoxon, Uji Independent Sample t-test, dan Uji Effect Size.Hasil penelitian ini menunjukkan bahwa penggunaan model pembelajaran Argument-Driven Inquiry (ADI) berpengaruh dalam peningkatan kemampuan argumentasi siswa pada topik keanekaragaman hayati. Hal ini berarti siswa berhasil mencapai tujuan-tujuan yang ditargetkan dalam pembelajaran model ADI. Kata kunci: model Argument-Driven Inquiry (ADI), kemampuan argumentasi siswa