bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression and clinical significance of NCOA5 in epithelial ovarian cancer
Authors: Song Xiaoping , Qian Da , Dai Ping , Li Qian , Xi Qiuping , Sun Kailv 
Published: May 2023

BackgroundNuclear receptor coactivator 5 (NCOA5) plays a significant role in the progression of human cancer. However, its expression in epithelial ovarian cancer (EOC) is unknown. The current study was designed to explore to investigate the clinical significance of NCOA5 and its correlation with the prognosis of EOC.MethodsImmunohistochemistry was used to detect the expression of NCOA5 in 60 patients with EOC in this retrospective study and statistical analysis was performed to assess its relevance to clinicopathologic features and survival.ResultsNCOA5 expression was significantly higher in EOC than in normal ovarian tissues (P &lt; 0.001). Its expression level was significantly correlated with FIGO stage (P &lt;0. 05) and subtypes of ovarian cancer (P &lt; 0.001), while not correlation with age , differentiation and lymph node metastasis (P&gt;0.05). Correlation analysis showed that NCOA5 was significantly correlated with CA125 (P &lt; 0.001) and HE4 (P &lt; 0.01). In a Kaplan-Meier analysis of overall survival rates, the patients with low expression of NCOA5 had significantly longer survival than high expression of NCOA5 (p=0.038).ConclusionNCOA5 high expression is associated with EOC progression and can be an independent factor affecting the prognosis of EOC patients.
Preoperative prediction model for macrotrabecular-massive hepatocellular carcinoma based on contrast-enhanced CT and clinical characteristics: a retrospective study
Authors: He Chutong , Zhang Wanli , Zhao Yue , Li Jiamin , Wang Ye , Yao Wang , Wang Nianhua , Ding Wenshuang , Wei Xinhua , Yang Ruimeng , Jiang Xinqing 
Published: May 2023

ObjectiveTo investigate the predictive value of contrast-enhanced computed tomography (CECT) imaging features and clinical factors in identifying the macrotrabecular-massive (MTM) subtype of hepatocellular carcinoma (HCC) preoperatively.MethodsThis retrospective study included 101 consecutive patients with pathology-proven HCC (35 MTM subtype vs. 66 non-MTM subtype) who underwent liver surgery and preoperative CECT scans from January 2017 to November 2021. The imaging features were evaluated by two board-certified abdominal radiologists independently. The clinical characteristics and imaging findings were compared between the MTM and non-MTM subtypes. Univariate and multivariate logistic regression analyses were performed to investigate the association of clinical-radiological variables and MTM-HCCs and develop a predictive model. Subgroup analysis was also performed in BCLC 0-A stage patients. Receiver operating characteristic (ROC) curves analysis was used to determine the optimal cutoff values and the area under the curve (AUC) was employed to evaluate predictive performance.ResultsIntratumor hypoenhancement (odds ratio [OR] = 2.724; 95% confidence interval [CI]: 1.033, 7.467; p = .045), tumors without enhancing capsules (OR = 3.274; 95% CI: 1.209, 9.755; p = .03), high serum alpha-fetoprotein (AFP) (≥ 228 ng/mL, OR = 4.101; 95% CI: 1.523, 11.722; p = .006) and high hemoglobin (≥ 130.5 g/L; OR = 3.943; 95% CI: 1.466, 11.710; p = .009) were independent predictors for MTM-HCCs. The clinical-radiologic (CR) model showed the best predictive performance, achieving an AUC of 0.793, sensitivity of 62.9% and specificity of 81.8%. The CR model also effectively identify MTM-HCCs in early-stage (BCLC 0-A stage) patients.ConclusionCombining CECT imaging features and clinical characteristics is an effective method for preoperatively identifying MTM-HCCs, even in early-stage patients. The CR model has high predictive performance and could potentially help guide decision-making regarding aggressive therapies in MTM-HCC patients.
A pan-cancer analysis of ABI3BP: a potential biomarker for prognosis and immunoinfiltration
Authors: Feng Yan , Tao Fengying , Qiao Han , Tang Huaping 
Published: May 2023

ObjectiveABI Family Member 3 Binding Protein (ABI3BP) is an extracellular matrix protein that affects the carcinogenesis of lung and esophageal cancer. However, the relevance of ABI3BP in different forms of cancer is uncertain.MethodsABI3BP expression was interpreted using the Cancer Genome Atlas (TCGA) database, the Genotype Tissue Expression Atlas (GTEx) database, the Human Protein Atlas (HPA) database, the Cancer Cell Line Encyclopedia (CCLE) database, and immunohistochemistry. The R programming language was used to analyze the association between ABI3BP expression and patient prognosis and evaluate the relationship between ABI3BP and the immune characteristics of tumors. Using the GDSC and CTRP databases, a drug sensitivity analysis of ABI3BP was conducted.ResultsABI3BP mRNA expression was shown by differential analysis to be down-regulated in 16 tumor types relative to normal tissues, corresponding with its protein expression level as determined by immunohistochemistry. Abnormal expression of ABI3BP accurately predicts the prognosis of patients with renal chromophobe carcinoma (KICH), mesothelioma (MESO), and pancreatic adenocarcinoma (PAAD). Meanwhile, aberrant expression of ABI3BP was associated with immune checkpoints, TMB, MSI, tumor purity, HRD, LOH, and drug sensitivity. A correlation between ABI3BP expression and the amount of infiltration of several immune-related cells in pan-cancer was determined by Immune Score, Stromal Score, and Estimated Score.ConclusionOur results show that ABI3BP might be employed as a molecular biomarker to predict prognosis, treatment susceptibility, and immunological response in patients with pan-cancer.
Chromosome Asynapsis Is the Main Cause of Male Sterility in the Interspecies Hybrids of East Asian Voles (Alexandromys, Rodentia, Arvicolinae)
Authors: Bikchurina Tatiana , Pavlenko Marina , Kizilova Elena , Rubtsova Daria , Sheremetyeva Irina , Kartavtseva Irina , Torgasheva Anna , Borodin Pavel 
Published: May 2023

Closely related mammalian species often have differences in chromosome number and morphology, but there is still a debate about how these differences relate to reproductive isolation. To study the role of chromosome rearrangements in speciation, we used the gray voles in the Alexandromys genus as a model. These voles have a high level of chromosome polymorphism and substantial karyotypic divergence. We investigated testis histology and meiotic chromosome behavior in the captive-bred colonies of Alexandromys maximowiczii, Alexandromys mujanensis, two chromosome races of Alexandromys evoronensis, and their interracial and interspecies hybrids, to explore the relationship between karyotypic differences and male hybrid sterility. We found that the seminiferous tubules of the males of the parental species and the interracial hybrids, which were simple heterozygotes for one or more chromosome rearrangements, contained germ cells at all stages of spermatogenesis, indicating their potential fertility. Their meiotic cells displayed orderly chromosome synapsis and recombination. In contrast, all interspecies male hybrids, which were complex heterozygotes for a series of chromosome rearrangements, showed signs of complete sterility. Their spermatogenesis was mainly arrested at the zygotene- or pachytene-like stages due to the formation of complex multivalent chains, which caused extended chromosome asynapsis. The asynapsis led to the silencing of unsynapsed chromatin. We suggest that chromosome asynapsis is the main cause of meiotic arrest and male sterility in the interspecies hybrids of East Asian voles.
A Novel Nitrogen-Fixing Bacterium Raoultella electrica Isolated from the Midgut of the Leafhopper Recilia dorsalis
Authors: Huang Qiuyan , Feng Yilu , Shan Hong-Wei , Chen Jian-Ping , Wu Wei 
Published: May 2023

Nitrogen is a crucial element for the growth and development of insects, but herbivorous insects often suffer from nitrogen nutrition deficiencies in their diets. Some symbiotic microorganisms can provide insect hosts with nitrogen nutrition through nitrogen fixation. Extensive research has clearly demonstrated the process of nitrogen fixation by symbiotic microorganisms in termites, while evidence supporting the occurrence and significance of nitrogen fixation in the diets of the Hemiptera is less conclusive. In this study, we isolated a strain of R. electrica from the digestive tract of a leafhopper, R. dorsalis, and found that it had nitrogen-fixing capabilities. Fluorescence in situ hybridization results showed that it was located in the gut of the leafhopper. Genome sequencing revealed that R. electrica possessed all the genes required for nitrogen fixation. We further evaluated the growth rate of R. electrica in nitrogen-containing and nitrogen-free media and measured its nitrogenase activity through an acetylene reduction assay. The findings of these studies could shed light on how gut microbes contribute to our understanding of nitrogen fixation.
Soybean Oil Regulates the Fatty Acid Synthesis II System of Bacillus amyloliquefaciens LFB112 by Activating Acetyl-CoA Levels
Authors: Cheng Qiang , Li Zhongxuan , Zhang Jing , Guo Henan , Ahmat Marhaba , Cheng Junhao , Abbas Zaheer , Hua Zhengchang , Wang Junyong , Tong Yucui , Yang Tiantian , Si Dayong , Zhang Rijun 
Published: May 2023

[Background] Bacillus LFB112 is a strain of Bacillus amyloliquefaciens screened in our laboratory. Previous studies found that it has a strong ability for fatty acid metabolism and can improve the lipid metabolism of broilers when used as feed additives. [Methods] This study aimed to confirm the fatty acid metabolism of Bacillus LFB112. Sterilized soybean oil (SSO) was added to the Beef Peptone Yeast (BPY) medium, and its effect on fatty acid content in the supernatant and bacteria, as well as expression levels of genes related to fatty acid metabolism, were studied. The control group was the original culture medium without oil. [Results] Acetic acid produced by the SSO group of Bacillus LFB112 decreased, but the content of unsaturated fatty acids increased. The 1.6% SSO group significantly increased the contents of pyruvate and acetyl-CoA in the pellets. Furthermore, the mRNA levels of enzymes involved in the type II fatty acid synthesis pathway of FabD, FabH, FabG, FabZ, FabI, and FabF were up-regulated. [Conclusions] Soybean oil increased the content of acetyl-CoA in Bacillus LFB112, activated its type II fatty acid synthesis pathway, and improved the fatty acid metabolism level of Bacillus LFB112. These intriguing results pave the way for further investigations into the intricate interplay between Bacillus LFB112 and fatty acid metabolism, with potential applications in animal nutrition and feed additive development.
Toxicity and Impact of Silica Nanoparticles on the Configuration of Gut Microbiota in Immunodeficient Mice
Authors: Shabbir Sana , Hu Yanzhou , He Xiaoyun , Huang Kunlun , Xu Wentao 
Published: May 2023

Nanoparticles (NPs), having exceptional physicochemical and electrical characteristics with lower toxicity, have evolved as dynamic drug delivery carriers in living organisms. Potentially, the intragastric gavage of silica nanoparticles (SiNPs) affects gut microbiota profiles in immunodeficient mice. In this study, the impact of SiNPs of variable size and dosage was investigated in cyclophosphamide (Cy)-induced immunodeficient mice, specifically on their immune functions and gut microbiota, through physicochemical and metagenomic analysis. SiNPs of different sizes and doses were gavaged to Cy-induced immunodeficient mice for 12 days at an interval of 24 h to investigate their effects on immunological functions and the gut microbiome of mice. Our results showed that SiNPs had no significant toxicological effects on the cellular and hematological activities of immunodeficient mice. Furthermore, after the administration of different levels of SiNPs, no immune dysfunction was found in the immunosuppressed mice groups. However, gut-microbial studies and comparisons of characteristic bacterial diversity and compositions demonstrated that SiNPs significantly affect the abundance of different bacterial communities. LEfSe analysis revealed that SiNPs significantly increased the abundance of Lactobacillus, Sphingomonas, Sutterella, Akkermansia, and Prevotella, and potentially reduced Ruminococcus and Allobaculum. Thus, SiNPs significantly regulate and modify the configuration of the gut microbiota in immunodeficient mice. These dynamic variations in the intestinal bacterial community, abundance, and diversity provide new insight into the regulation and administration of silica-based NPs. This would be helpful for the further demonstration of the mechanism of action and prediction of the potential effects of SiNPs.
Advances in Biotechnological Production and Metabolic Regulation of Astragalus membranaceus
Authors: Ji Baoyu , Xuan Liangshuang , Zhang Yunxiang , Zhang Guoqi , Meng Jie , Mu Wenrong , Liu Jingjing , Paek Kee-Yoeup , Park So-Young , Wang Juan , Gao Wenyuan 
Published: May 2023

Legume medicinal plants Astragalus membranaceus are widely used in the world and have very important economic value, ecological value, medicinal value, and ornamental value. The bioengineering technology of medicinal plants is used in the protection of endangered species, the rapid propagation of important resources, detoxification, and the improvement of degraded germplasm. Using bioengineering technology can effectively increase the content of secondary metabolites in A. membranaceus and improve the probability of solving the problem of medicinal plant resource shortage. In this review, we focused on biotechnological research into A. membranaceus, such as the latest advances in tissue culture, including callus, adventitious roots, hairy roots, suspension cells, etc., the metabolic regulation of chemical compounds in A. membranaceus, and the research progress on the synthetic biology of astragalosides, including the biosynthesis pathway of astragalosides, microbial transformation of astragalosides, and metabolic engineering of astragalosides. The review also looks forward to the new development trend of medicinal plant biotechnology, hoping to provide a broader development prospect for the in-depth study of medicinal plants.
Subcapsular Hepatic Hematoma Post Endoscopic Retrograde Cholangiopancreatography (ERCP): a case report
Authors: Dos Santos Mateus Araújo , Dos Santos Filho Paulo Vicente , Menezes Victor Cury , Fornari Jennifer Camila de Souza , Morita Tiago Oliveira 
Published: May 2023

Subcapsular hepatic hematoma is a rare complication found after the ERCP, which can lead to abdominal pain, anemia, fever and hypotension. The case of a 34-year-old patient who developed a subcapsular liver hematoma after ERCP is presented in this paper. The patient had abdominal pain and anemia, which did not improve with conservative treatment. Drainage therapy was performed, leading to remission of the condition.
Hyper Low-cost Ultimate Rice Mechanization System
Authors: Ito Nobutaka 
Published: May 2023

About 480 million tons of rice (milled rice base) are produced annually in the world. It is one of the cereals with a low trade rate for its production. In Asia, more than 90% of the world's production is produced. The top 10 countries in rice production are all Asian countries. The main operation of rice cultivation consists of planting, management while rice growing (weed & pest control, water management), harvesting, and post-harvest processing. In addition to infrastructure development such as irrigation and drainage system through field reclamation, the development of chemical fertilizers, herbicides, and pesticides contributed to labor saving. However, the introduction of the combine harvester, which can significantly save labor and simplify the harvesting operation, is one of the required parts of the technological innovation.
In this paper, one direction is shown to realize hyper-low cost of rice cultivation system through these technological innovations, demand and consumption increase of rice, and open up new markets as a response to the future food crisis.
Computational molecular structure analysis, electronic properties of sudan dye doped thioureabarium chloride crystals for nlo Applications
Authors: Nithiyanantham Subramanian , Panimalar S , Kumaresan P , Sambath Kumar K 
Published: May 2023

Pure thiourea barium chloride (TBC) crystals were grown by slow evaporation from aqueous solution and slow cooling (0.1oC/ day). The growth was carried out for 21 days by keeping the bath at a temperature of 39oC. Optimal molecular structures are explored by DFT / B3LYP method with 6-31G (d, p) based synthesis. Hyper conjugative interactions, charge delocalization and intra molecular hydrogen bonding have been analyzed using natural bond orbital (NBO) analysis. Moreover lower in the HOMO and LUMO energy gap explains the eventual charge transfer interactions taking place within the molecule, which influences the biological activity of the compound and also energy serves as a measure of the excitability of a compound, the smaller the energy gap, the more easily the compound will be excited. Electronic structures were discussed and the displacement of the electron density was determined. Second – order perturbation theory analysis of the intra molecular bonds of Methyl Orange doped TBC were derived.
Сравнительный анализ протеомных карт дентинного ликвора: роль биомаркеров в молекулярной диагностике болезней пульпы зубов
Authors: Mitronin A. V. , Ostanina D. A. , Mitronin Y. A. , Mikhailova A. E. , Wolgin M. 
Published: May 2023

Цель. Провести сравнительный анализ протеомного профиля дентинной жидкости в норме и при болезнях пульпы с целью выявления пептидных маркеров воспаления для усовершенствования методов диагностики состояния пульпы зубов.Материалы и методы. На клиническом этапе исследования были получены 48 образцов дентинного ликвора, забор которых проводили с помощью нитроцеллюлозной мембраны по разработанной методике, и 48 соответствующих им удаленных зубов, которые подвергались гистологическому исследованию с целью подтверждения клинического диагноза. В лабораторный этап было включено 45 проб дентинной жидкости для проведения протеомного анализа хромато-масс-спектрометрическим методом.Результаты. При протеомном анализе выявлено 206 белковых фракций в дентинном ликворе зубов. Совпадение протеомов дентинной жидкости в норме, при начальном и остром воспалении пульпы составило 19%, или 39 белковых фракций. Определен специфичный белковый маркер начальной стадии воспаления – альфа-1- антихимотрипсин, количество которого достоверно повышается в 48 раз при начальном пульпите (p < 0,05).Выводы. По результатам исследования были созданы протеомные карты дентинного ликвора в норме и при воспалении пульпы. Было выявлено, что белковую фракцию альфа-1-антихимотрипсин целесообразно рассматривать в качестве специфического маркера воспаления при болезнях пульпы, в частности, для диагностики начального пульпита.
Sistem Informasi Pemesanan Jasa Fotografi pada Julian Photography Menggunakan Framework Laravel
Authors: Pradipta Wistika Ketut , Pramana Dian , Setiasih Ni Wayan 
Published: May 2023

Julian Photography merupakan salah satu usaha jasa fotografi yang berlokasi di Kabupaten Jembrana, Bali. Selain menyediakan jasa fotografi, julian photography juga melayani jasa video, printing dan penjualan bingkai foto. Saat ini, proses pemesanan pada Julian Photography masih dilakukan secara konvensional dimana pemesanan dilakukan oleh pelanggan dengan datang langsung ke toko. Hal ini mengakibatkan pelanggan kesulitan dalam melakukan transaksi dan pegawai kesulitan dalam mengelola data transaksi. Oleh karena itu, pada penelitian ini dikembangkan aplikasi sistem pemesanan fotografi menggunakan Laravel sebagai framework dari PHP. Dengan adanya aplikasi ini, diharapkan dapat membantu Julian Photography dalam mengelola data pemesanan, mengelola data pelanggan, dan memberikan info kepada pelanggan terkait produk dan jasa yang disediakan. Terdapat 5 tahapan dalam penelitian ini. Tahap pertama yakni pengumpulan data melalui observasi langsung pada objek yang diteliti, studi literatur serta wawancara kepada pemilik usaha. Tahapan kedua adalah analisis dari sisi pengguna, yakni analisis data yang dibutuhkan dan proses manajemen pengguna. Tahapan ketiga yaitu perancangan sistem menggunakan desain basis data konseptual, diagram konteks serta arsitektur web. Tahapan keempat yaitu implementasi sistem menggunakan bahasa pemrograman php dengan Laravel pada bagian front-end maupun back-end. Tahapan terakhir adalah pengujian sistem menggunakan metode Blackbox testing yang berfokus pada pengujian fungsional dari sistem. Hasil penelitian ini menghasilkan sistem pemesanan jasa fotografi berbasis website dengan akses pengguna yang telah ditentukan menggunakan Laravel.
Visuo-motor interference is modulated by task interactivity: A kinematic study
Authors: Rocca Matilde , Sacheli Lucia Maria , Romeo Luca , Cavallo Andrea 
Published: May 2023

AbstractExtensive evidence shows that action observation can influence action execution, a phenomenon often referred to as visuo-motor interference. Little is known about whether this effect can be modulated by the type of interaction agents are involved in, as different studies show conflicting results. In the present study, we aimed at shedding light on this question by recording and analyzing the kinematic unfolding of reach-to-grasp movements performed in interactive and noninteractive settings. Using a machine learning approach, we investigated whether the extent of visuo-motor interference would be enhanced or reduced in two different joint action settings compared with a noninteractive one. Our results reveal that the detrimental effect of visuo-motor interference is reduced when the action performed by the partner is relevant to achieve a common goal, regardless of whether this goal requires to produce a concrete sensory outcome in the environment (joint outcome condition) or only a joint movement configuration (joint movement condition). These findings support the idea that during joint actions we form dyadic motor plans, in which both our own and our partner’s actions are represented in predictive terms and in light of the common goal to be achieved. The formation of a dyadic motor plan might allow agents to shift from the automatic simulation of an observed action to the active prediction of the consequences of a partner’s action. Overall, our results demonstrate the unavoidable impact of others’ action on our motor behavior in social contexts, and how strongly this effect can be modulated by task interactivity.