bluuebunny/crossref_metadata_2025_split huggingface dataset:
Near‐Infrared Emissive Cascaded Artificial Light‐Harvesting System with Enhanced Antibacterial Efficiency
Authors: Chen Maowen , Lu Zhenni , Li Man , Jiang Bei , Liu Senkun , Li Yinuo , Zhang Bangrui , Li Xianying , Yi Tao , Zhang Dengqing 
Published: May 2023

AbstractCombination of platinum(II) metallacycles and photodynamic inactivation presents a promising antibacterial strategy. Herein, a cascaded artificial light‐capturing system is developed in which an aggregation‐induced emission‐active platinum(II) metallacycle (PtTPEM) is utilized as the antenna, sulforhodamine 101 (SR101) as a key conveyor, and the near‐infrared emissive photosensitizer Chlorin‐e6 (Ce6) as the final energy acceptor. The well‐dispersed Ce6 in the proximity of energy donors not only avoids self‐quenching in the physiological environment but also contributes to energy transfer from donor to acceptor, thereby significantly improving the 1O2 generation ability of the light‐harvesting system under white light irradiation. By integrating the platinum(II) metallacycle and 1O2, a more efficient synergistic antibacterial effect is achieved at low concentrations, along with a significant decrease in dark toxicity caused by PtTPEM.
Drycells: Cell‐Suspension Micro Liquid Marbles for Single‐Cell Picking
Authors: Tenjimbayashi Mizuki , Yamamoto Shota , Uto Koichiro 
Published: April 2023

AbstractCell‐picking technology is essential for cell culturing. Although the recently developed tools enable single‐cell‐level picking, they rely on special skills or additional devices. In this work, a dry powder that encapsulates single to several cells with a >95% aqueous culture medium, thereby acting as a powerful cell‐picking tool, is reported. The proposed “drycells” are formed by spraying a cell suspension onto a powder bed of hydrophobic fumed silica nanoparticles. The particles adsorb to the droplet surface and form a superhydrophobic shell, which prevents the drycells from coalescence. The number of encapsulated cells per drycell can be controlled by adjusting the drycell size and cell‐suspension concentration. Moreover, it is possible to encapsulate a pair of normal or cancerous cells and create several cell colonies within a single drycell. A sieving process can be used to sort the drycells according to size. The size of the droplet can range from one to hundreds of micrometers. The drycells are sufficiently stiff to be collected using tweezers; however, centrifugation separates them into nanoparticles and cell‐suspension layers, with the separated particles being recyclable. Various handling techniques, such as splitting coalescence and inner liquid replacement, can be used. It is believed that the application of the proposed drycells will greatly improve the accessibility and productivity of single‐cell analysis.
Defect Pyrochlore‐Type Mott–Schottky Photocatalysts for Enhanced Ammonia Synthesis at Low Pressure
Authors: Zhao Zihan , Tan Ruike , Kong Yuxiang , Zhang Zihao , Qiu Shou , Mu Xiaoyue , Li Lu 
Published: April 2023

AbstractThe ambient ammonia synthesis coupled with distributed green hydrogen production technology can provide promising solutions for low‐carbon NH3 production and H2 storage. Herein, we reported Ru‐loaded defective pyrochlore K2Ta2O6−x with remarkable visible‐light absorption and a very low work function, enabling effective visible‐light‐driven ammonia synthesis from N2 and H2 at low pressure down to 0.2 atm. The photocatalytic rate was 2.8 times higher than that of the best previously reported photocatalyst and the photo‐thermal rate at 425 K was similar to that of Ru‐loaded black TiO2 at 633 K. Compared to perovskite‐type KTaO3−x with the same composition, the pyrochlore exhibited a 3.7‐fold increase in intrinsic activity due to a higher photoexcited charge separation efficiency and a higher conduction band position. The interfacial Schottky barrier and spontaneous electron transfer between K2Ta2O6−x and Ru further improve photoexcited charge separation and accumulate energetic electrons to facilitate N2 activation.
Untersuchung von Ladungstransferprozessen in einer kovalent verknüpften [Ge9]‐Cluster‐Imin Dyade
Authors: Wallach Christoph , Selic Yasmin , Geitner Felix S. , Kumar Ajeet , Thyrhaug Erling , Hauer Jürgen , Karttunen Antti J. , Fässler Thomas F. 
Published: April 2023

AbstractC60 Donor Dyaden, in denen der Kohlenstoffkäfig kovalent an eine Elektronen‐Donoreinheit gebunden vorliegt, werden als ein mögliches Elektronentransfersystem diskutiert, und es wurde gezeigt, dass kugelförmige [Ge9]‐Clusteranionen bezüglich ihrer elektronischen Struktur eng mit den Fullerenen verwandt sind. Die optischen Eigenschaften dieser Cluster und ihrer funktionalisierten Derivate sind jedoch nahezu unbekannt. Wir berichten nun über die Synthese des intensiv roten [Ge9]‐Clusters, welcher an ein ausgedehntes π‐Elektronensystem gebunden ist. [Ge9{Si(TMS)3}2{CH3C=N}‐DAB(II)Dipp]− (1−) entsteht bei der Reaktion von [Ge9{Si(TMS)3}2]2− mit Bromdiazaborol DAB(II)Dipp‐Br in CH3CN (TMS=Trimethylsilyl; DAB(II)=1,3,2‐Diazaborol mit einem ungesättigten Gerüst; Dipp=2,6‐Di‐iso‐propylphenyl). Die reversible Protonierung der Imineinheit in 1− führt zum tiefgrünen, zwitterionischen Cluster [Ge9{Si(TMS)3}2{CH3C=N(H)}‐DAB(II)Dipp] (1‐H). Optische Spektroskopie in Kombination mit zeitabhängigen Dichtefunktional‐theoretischen Berechnungen legen eine Elektronentransfer‐Anregung zwischen dem Cluster und dem antibindenden π*‐Orbital der Iminiumeinheit als Urache für die intensive Farbe nahe. Ein Absorptionsmaximum von 1‐H im roten Bereich des elektromagnetischen Spektrums und der entsprechende niedrigste angeregte Zustand bei λ=669 nm machen die Verbindung zu einem interessanten Ausgangspunkt für weitere Untersuchungen, die auf das Design photoaktiver Clusterverbindungen abzielen.
Probing Charge‐Transfer Processes in a Covalently Linked [Ge9]‐Cluster Imine Dyad
Authors: Wallach Christoph , Selic Yasmin , Geitner Felix S. , Kumar Ajeet , Thyrhaug Erling , Hauer Jürgen , Karttunen Antti J. , Fässler Thomas F. 
Published: April 2023

AbstractC60 donor dyads in which the carbon cage is covalently linked to an electron‐donating unit have been discussed as one possibility for an electron‐transfer system, and it has been shown that spherical [Ge9] cluster anions show a close relation to fullerenes with respect to their electronic structure. However, the optical properties of these clusters and of functionalized cluster derivatives are almost unknown. We now report on the synthesis of the intensely red [Ge9] cluster linked to an extended π‐electron system. [Ge9{Si(TMS)3}2{CH3C=N}‐DAB(II)Dipp]− (1−) is formed upon the reaction of [Ge9{Si(TMS)3}2]2− with bromo‐diazaborole DAB(II)Dipp‐Br in CH3CN (TMS=trimethylsilyl; DAB(II)=1,3,2‐diazaborole with an unsaturated backbone; Dipp=2,6‐di‐iso‐propylphenyl). Reversible protonation of the imine entity in 1− yields the deep green, zwitterionic cluster [Ge9{Si(TMS)3}2{CH3C=N(H)}‐DAB(II)Dipp] (1‐H) and vice versa. Optical spectroscopy combined with time‐dependent density functional theory suggests a charge‐transfer excitation between the cluster and the antibonding π* orbital of the imine moiety as the cause of the intense coloration. An absorption maximum of 1‐H in the red region of the electromagnetic spectrum and the corresponding lowest‐energy excited state at λ=669 nm make the compound an interesting starting point for further investigations targeting the design of photo‐active cluster compounds.
DetectingLegionella pneumophilain Cooling Tower Water Samples with a DNAzyme/Bead‐Based Fluorescence Assay
Authors: Qian Shuwen , McConnell Erin M. , Rothenbroker Meghan , Gu Jimmy , Alungulesa Simina , Godbout Louis , Li Yingfu 
Published: April 2023

AbstractLegionella pneumophilais the causative agent behind the deadly waterborne disease Legionnaires’, which is commonly transmitted by the spread of contaminated droplets from cooling tower water samples. The lack of effective detection methods presents a challenge forL. pneumophilaoutbreak control. Previously, an RNA‐cleaving DNAzyme called LP1 was reported to specifically targetL. pneumophila. In this study, LP1 was immobilized onto agarose beads via streptavidin‐biotin interaction to develop a bead‐based fluorescence assay forL. pneumophiladetection. This bead‐based assay demonstrated excellent stability and functionality in various cooling tower water samples. To improveL. pneumophilamonitoring in real‐world samples, a lysozyme treatment was used to enhanceL. pneumophilarecognition. The limit of detection of this DNAzyme‐based bead assay can reach 103 CFUs in cell‐spiked cooling tower water samples without cell culturing or signal amplification steps.
Functionalization of electrospun nanofiber for biomedical application
Authors: Chakrapani Gayathri , Ramakrishna Seeram , Zare Mina 
Published: April 2023

AbstractFor centuries, nanofibers have been fabricated by converting polymeric materials into nanofibers with tunable mixtures, dimensions, pore structure, and interface properties. Nanofibers include many benefits for transporting drug substances with various biomedical applications. Electrospun nanofibers with customizable geometries and release properties are readily formed due to advances in nanotechnology. The structural characteristics of the electrospun nanofibers/nano yarns are tremendous owing to their large surface‐to‐volume ratio, interconnected pores, lightweight and permeability, which helps in their wide applications in the health care, energy and environmental aspects of research. The present review focuses on the recent advances in electrospun nanofibers for biomedical applications. Further, this review will examine nanofiber uses in therapeutic delivery and tissue engineering. Additionally, the potential biomedical applications of nanofibers in wound healing and cancer research are reviewed.
Prediction of terephthalic acid yield in aqueous hydrolysis of polyethylene terephthalate
Authors: Abedsoltan Hossein , Zoghi Zeinab , Mohammadi Amir H. 
Published: April 2023

AbstractAqueous hydrolysis is used to chemically recycle polyethylene terephthalate (PET) due to production of high‐quality terephthalic acid (TPA), the PET monomer. PET hydrolysis depends on various factors including PET size, catalyst concentration, and reaction temperature. So, modeling PET hydrolysis by considering the effective factors can provide useful information for material researchers to specify how to design and run these reactions. It will save time, energy, and materials by optimizing the hydrolysis conditions. Machine learning algorithms enable to design models to predict the output results. For the first time, 381 experimental data were gathered to model aqueous hydrolysis of PET. Effective factors on PET hydrolysis were connected to the TPA yield. The logistic regression was applied to rank the effective factors. Two algorithms were proposed, artificial neural network multi‐layer perceptron (ANN‐MLP) and adaptive network‐based fuzzy inference system (ANFIS). The dataset was divided into training, validating, and testing sets to train, validate, and test the models, respectively. The models predicted TPA yield sufficiently where the ANFIS model outperformed. R‐squared (R2) and Root Mean Square Error (RMSE) loss functions were employed to measure the efficiency of the models and evaluate their performance.
Enhancing thermal stability of polycarbonate films with one‐sided coating of graphene oxide
Authors: Nguyen Thao , Wei Jacob 
Published: May 2023

AbstractIn this study, polycarbonate (PC) films were coated with a thin layer of graphene oxide (GO) or its reduced form (rGO) via vacuum filtration. The effects of the coating on PC were studied by means of modulated differential scanning calorimetry (mDSC), thermogravimetric analysis (TGA), and scanning electron microscopy. As shown by mDSC, there was no significant change in glass transition temperature (Tg), indicating little molecular interaction between the PC and the coating. In addition, GO and rGO showed poorer thermal stability than the neat PC, which is consistent with the literature. Despite these findings, both GO‐ and rGO‐coated PC films exhibited significantly higher onset thermal degradation temperatures than the neat PC. Moreover, when the rGO loading was below a certain level, the coated PC films demonstrated a comprehensive enhancement in thermal stability over the entire temperature range of TGA measurement. Such enhancement could be observed even at very low rGO loadings when the surface of the PC film was partially coated.
Groundwater treatment and disinfection by electrochemical advanced oxidation process: Influence of the supporting electrolyte and the nature of the contaminant
Authors: Soares Beatriz S. , de Mello Rodrigo , Motheo Artur J. 
Published: April 2023

AbstractThe United Nations (UN) has considered water a human right since 1977. However, freshwater available for consumption represents less than 1% of all water on Earth. Groundwater represents one of the largest reserves of drinking water and is susceptible to chemical contamination, especially from pollutants that seep into the soil, such as atrazine, bisphenol A, and tetracycline. These substances, along with Escherichia coli, were selected to simulate contamination in groundwater samples and evaluate the efficiency of electrochemical oxidation using boron‐doped diamond anodes and four different anion salts to analyze their impact on the treatment process. After electrolysis, the degradation of tetracycline, bisphenol A and atrazine was found to increase with decreasing current density, with average values of 77%, 96% and 100% at 15 mA cm−2 and 68%, 83% and 99% at 35 mA cm−2, respectively. Moreover, the mineralization of these substances showed the same behavior, decreasing from 67%, 64%, and 54% at 15 mA cm−2 to 52%, 35%, and 49% at 35 mA cm−2. The analysis of the results showed that the ions present in the solution significantly affect the degradation process and that they interact with the impurities used. For atrazine and tetracycline, the degradation efficiency followed the same pattern,  <  <  < . However, in the case of bisphenol A and E. coli, phosphate showed better results, similar to nitrate. The high efficiency in eliminating bacteria, even at high concentrations, shows that the electrochemical treatment system has a strong bactericidal effect, eliminating bacterial colonies with up to 5 min of treatment.
Critical challenges and advances in recombinant adeno‐associated virus (rAAV) biomanufacturing
Authors: Fu Qiang , Polanco Ashli , Lee Yong Suk , Yoon Seongkyu 
Published: May 2023

AbstractGene therapy is a promising therapeutic approach for genetic and acquired diseases nowadays. Among DNA delivery vectors, recombinant adeno‐associated virus (rAAV) is one of the most effective and safest vectors used in commercial drugs and clinical trials. However, the current yield of rAAV biomanufacturing lags behind the necessary dosages for clinical and commercial use, which embodies a concentrated reflection of low productivity of rAAV from host cells, difficult scalability of the rAAV‐producing bioprocess, and high levels of impurities materialized during production. Those issues directly impact the price of gene therapy medicine in the market, limiting most patients’ access to gene therapy. In this context, the current practices and several critical challenges associated with rAAV gene therapy bioprocesses are reviewed, followed by a discussion of recent advances in rAAV‐mediated gene therapy and other therapeutic biological fields that could improve biomanufacturing if these advances are integrated effectively into the current systems. This review aims to provide the current state‐of‐the‐art technology and perspectives to enhance the productivity of rAAV while reducing impurities during production of rAAV.
Identification of an EMT‐related gene‐based prognostic signature in osteosarcoma
Authors: Gong Haoli , Tao Ye , Xiao Sheng , Li Xin , Fang Ke , Wen Jie , Zeng Ming , Liu Yiheng , Chen Yang 
Published: April 2023

AbstractBackgroundThe correlation between epithelial‐mesenchymal transition (EMT) and osteosarcoma (OS) has been widely reported. Integration of the EMT‐related genes to predict the prognosis is significant for investigating the mechanism of EMT in OS. Here, we aimed to construct a prognostic EMT‐related gene signature for OS.MethodsTranscriptomic and survival data of OS patients were downloaded from Therapeutically Applicable Research to Generate Effective Treatments (TARGET) and Gene Expression Omnibus (GEO). We performed univariate Cox regression, least absolute shrinkage and selection operator (LASSO) regression, and stepwise multivariate Cox regression analysis to construct EMT‐related gene signatures. Kaplan–Meier analysis and time‐dependent receiver operating characteristic (ROC) were applied to evaluate its predictive performance. GSVA, ssGSEA, ESTIMATE, and scRNA‐seq were performed to investigate the tumor microenvironment, and the correlation between IC50 of drugs and ERG score was investigated. Furthermore, Edu and transwell experiments were conducted to assess the malignancy of OS cells.ResultsWe constructed a novel EMT‐related gene signature (including CDK3, MYC, UHRF2, STC2, COL5A2, MMD, and EHMT2) for outcome prediction of OS. According to the signature, patients stratified into high‐ and low‐ERG‐score groups exhibited significantly different prognoses. ROC curves and Kaplan–Meier analysis revealed a promising performance of the signature with external validation. GSVA, ssGSEA, ESTIMATE algorithm, and scRNA‐seq excavated EMT‐related pathways and suggested the correlation between ERG score and immune activation. Notably, the pivotal gene CDK3 was upregulated in OS tissue and positively related to OS cell proliferation and migration.ConclusionOur EMT‐related gene signature might reference OS risk stratification and guide clinical strategies as an independent prognostic factor in OS.
Survival in elderly patients with breast cancer with and without autoimmune disease
Authors: Dedousis Demitrios , Zhang Annie L. , Vassiliou Anastasia N. , Cao Shufen , Yammani Deepthi , Kyasaram Ravi K. , Shanahan John P. , Keinath Melissa C. , Hsu Melinda L. , Fu Pingfu , Dowlati Afshin , Montero Alberto J. 
Published: April 2023

AbstractBackgroundPatients with certain autoimmune conditions are at a reduced risk of developing breast cancer compared to the general population. Despite this, little is known about outcomes in patients with breast cancer who have a concurrent autoimmune diagnosis.MethodsThis study compared differences in outcomes between women with breast cancer who had or did not have an autoimmune diagnosis. The SEER‐Medicare databases (2007–2014) were used to identify patients with breast cancer and diagnosis codes were used to identify those with an autoimmune disorder.ResultsThe studied autoimmune diseases had a prevalence of 27% among the 137,324 patients with breast cancer. Autoimmune disease was associated with significantly longer overall survival (OS) and significantly lower cancer‐specific mortality (CSM) among stage IV breast cancer patients (p < 0.0001). After controlling for the effects of age, race, chronic kideny disease, chemotherapy, and radiation therapy autoimmune disease was still predictive of improved OS (HR: 1.45, 95% CI: 1.35–1.55, p < 0.0001) and CSM (HR: 1.40, 95% CI: 1.29–1.5, p < 0.0001). By contrast, in patients with stage I–III breast cancer, the presence of an autoimmune diagnosis was associated with a lower OS (p < 0.0001, p < 0.0001, and p = 0.026, respectively), compared to patients without autoimmune disease.ConclusionsWe found a higher prevalence of rheumatoid arthritis, Crohn's disease, ulcerative colitis, and systemic lupus erythematosus in patients with breast cancer compared to age matched cohorts in the general population. The presence of an autoimmune diagnosis was associated with a lower OS in stages I–III breast cancer and improved OS and CSM in patients with stage IV disease. These results suggest that anti‐tumor immunity plays an important role in late stage breast cancer and could potentially be exploited to improve the effectiveness of immunotherapy.
Resolving Confusion Surroundingd‐Ala‐d‐Ala Ligase Catalysis in Cyanobacterial Mycosporine‐Like Amino Acid (MAA) Biosynthesis
Authors: Dextro Rafael B. , Fiore Marli F. , Long Paul F. 
Published: April 2023

AbstractMycosporine‐like amino acids (MAAs) are natural UV‐absorbing sunscreens that evolved in cyanobacteria and algae to palliate harmful effects from obligatory exposure to solar radiation. Multiple lines of evidence prove that in cyanobacteria all MAAs are derived from mycosporine‐glycine, which is typically modified by an ATP‐dependent ligase encoded by the genemysD. The function of the mysD ligase has been experimentally described but haphazardly named based solely upon sequence similarity to thed‐alanine‐d‐alanine ligase of bacterial peptidoglycan biosynthesis. Combining phylogeny and alpha‐fold tertiary protein structure prediction unambiguously distinguished mysD fromd‐alanine‐d‐alanine ligase. The renaming of mysD to mycosporine‐glycine‐amine ligase (MG‐amine ligase) using recognised enzymology rules of nomenclature is, therefore, proposed, and considers relaxed specificity for several different amino acid substrates. The evolutionary and ecological context of MG‐amine ligase catalysis merits wider appreciation especially when considering exploiting cyanobacteria for biotechnology, for example, producing mixtures of MAAs with enhanced optical or antioxidant properties.
Intraprocedural delayed reassessment of paravalvular regurgitation in TAVR significantly reduces the use of postdilatation
Authors: De la Torre Hernandez Jose M. , Veiga Fernandez Gabriela , Barrera Sergio , Sainz Laso Fermin , Lee Dae‐Hyun , Ruisanchez Villar Cristina , Lerena Piedad , Garcia Camarero Tamara , Cuesta Cosgaya Jose M. , Gil Ongay Aritz , Zueco Javier 
Published: May 2023

AbstractObjectivesWe sought to investigate the effect of a 15‐min delayed intraprocedural reassessment of paravalvular aortic regurgitation (PVR) after an immediate evaluation of posttranscatheter aortic valve replacement (TAVR) on the regurgitation grading and usage of postdilatation.BackgroundPVR after TAVR is associated with poor prognosis, but postdilatation may increase the risk of other complications.MethodsIn a prospective cohort of consecutive patients treated with balloon‐expandable valve ES‐3 ultra, the degree of PVR was assessed immediately and 15 min after that first evaluation (excluded severe cases), with the indication of postdilatation based on the delayed assessment. As a control group, the previous consecutive series of patients also treated with the same model of valve prosthesis was used.ResultsA total of 180 patients were included in the prospective study cohort and 152 in the retrospective control group. In the study group, the immediate PVR assessment showed none‐trace 27.5%, mild 52%, moderate 19%, and severe 1.5%, and the delayed re‐evaluation graded PVR as none‐trace 83%, mild 15.6%, and moderate 1.2% (p < 0.001 as compared to immediate). In the control group, the immediate PVR assessment showed none‐trace 33.5%, mild 52%, moderate 13%, and severe 1.5%. The rate of postdilatation was 2.8% in the study group versus 10.5% in the control group (p = 0.006). At discharge, no differences were observed between groups in PVR echocardiographic grading.ConclusionsA post‐TAVR delayed intraprocedural reassessment of the PVR shows a clearly lower degree of regurgitation as compared to immediate evaluation, which significantly decreased the indication of postdilatation.