bluuebunny/crossref_metadata_2025_split huggingface dataset:
Species-wide quantitative transcriptomes and proteomes reveal distinct genetic control of gene expression variation in yeast
Authors: Teyssonnière E. , Trébulle P. , Muenzner J. , Loegler V. , Ludwig D. , Amari F. , Mülleder M. , Friedrich A. , Hou J. , Ralser M. , Schacherer J. 
Published: September 2023

SummaryGene expression varies between individuals and corresponds to a key step linking genotypes to phenotypes. However, our knowledge regarding the species-wide genetic control of protein abundance, including its dependency on transcript levels, is very limited. Here, we have determined quantitative proteomes of a large population of 942 diverse natural Saccharomyces cerevisiae yeast isolates. We found that mRNA and protein abundances are weakly correlated at the population gene level. While the protein co-expression network recapitulates major biological functions, differential expression patterns reveal proteomic signatures related to specific populations. Comprehensive genetic association analyses highlight that genetic variants associated with variation in protein (pQTL) and transcript (eQTL) levels poorly overlap (3.6%). Our results demonstrate that transcriptome and proteome are governed by distinct genetic bases, likely explained by protein turnover. It also highlights the importance of integrating these different levels of gene expression to better understand the genotype-phenotype relationship.HighlightsAt the level of individual genes, the abundance of transcripts and proteins is weakly correlated within a species (ρ= 0.165).While the proteome is not imprinted by population structure, co-expression patterns recapitulate the cellular functional landscapeWild populations exhibit a higher abundance of respiration-related proteins compared to domesticated populationsLoci that influence protein abundance differ from those that impact transcript levels, likely because of protein turnover
Frequentmers - a novel way to look at metagenomic Next Generation Sequencing data and an application in detecting liver cirrhosis
Authors: Mouratidis Ioannis , Chantzi Nikol , Khan Umair , Konnaris Maxwell A. , Chan Candace S.Y , Mareboina Manvita , Georgakopoulos-Soares Ilias 
Published: September 2023

AbstractEarly detection of human disease is associated with improved clinical outcomes. However, many diseases are often detected at an advanced, symptomatic stage where patients are past efficacious treatment periods and can result in less favorable outcomes. Therefore, methods that can accurately detect human disease at a presymptomatic stage are urgently needed. Here, we introduce “frequentmers”; short sequences that are specific and recurrently observed in either patient or healthy control samples, but not in both. We showcase the utility of frequentmers for the detection of liver cirrhosis using metagenomic Next Generation Sequencing data from stool samples of patients and controls. We develop classification models for the detection of liver cirrhosis and achieve an AUC score of 0.91 using ten-fold cross-validation. A small subset of 200 frequentmers can achieve comparable results in detecting liver cirrhosis. Finally, we identify the microbial organisms in liver cirrhosis samples, which are associated with the most predictive frequentmer biomarkers.
Unveiling sustainable service innovations: exploring segmentation patterns in Ecuadorian restaurant sector
Authors: Montesdeoca Calderon María Gabriela , Gil-Saura Irene , Ruiz-Molina María-Eugenia , Martin-Rios Carlos 
Published: September 2023

PurposeThis paper aims to analyze the relationship between sustainability practices and the degree of innovation in the service provided by restaurants. The study identifies relevant restaurant segments in relation to sustainable practice-based service innovation so that effective actions to raise awareness and train managers and staff may be developed. Segmentation has been identified as a key tool when designing strategies and proposing actions. Yet, the use of segmentation techniques is still scarce regarding service innovation and sustainability in restaurants.Design/methodology/approachA segmentation analysis was carried out applying the CHAID algorithm from 300 valid questionnaires completed by restaurant owners or managers from coastal Ecuador, where tourism and gastronomy may be drivers of service innovation.FindingsA typology of restaurants based on the sustainability-service innovation interrelation suggests three final segments: sustainable innovators focused on the value chain, moderate innovators focused on saving resources and restaurants with a low innovative profile.Practical implicationsThe three segments derived from the analysis present differences in terms of the degree of implementation of sustainability practices, as well as in terms of the demographic profile of the restaurant manager. These segments are measurable, substantial, accessible and actionable, so that tailored initiatives to raise awareness and boost sustainability-oriented innovativeness among restaurant owners/managers may be targeted to each group of establishments.Originality/valueThe present research provides evidence of the positive relationship between sustainability practices and service innovation in foodservices. The segments of restaurants identified enable the design and implementation of actions that facilitate the transition of less sustainability-oriented restaurants towards more innovative and sustainable business models.
Identifying the factors influencing enterprise digital transformation intention: an empirical study based on net effects and joint effects
Authors: Zhang Wenkun , Chu Jinhua , Zhang Tao , Wang Yanan 
Published: September 2023

PurposeIn contrast to existing studies, this paper aims to propose that digital transformation does not depend on a single condition; rather, it depends on the interaction between internal and external factors of a firm. Therefore, the aim of this paper is to examine the effect of a combination of internal and external factors on a firm's digital transformation intention.Design/methodology/approachAn empirical analysis on a sample of 112 Chinese small- and medium-sized firms was conducted by applying smart-PLS and fuzzy set qualitative comparative analysis (fsQCA).FindingsThe results of smart PLS show that external pressures (institutional and market pressures) and human capital have a positive impact on corporate digital transformation intentions. From a combination perspective, the results of the fsQCA show that there are five causal conditions that lead to high digital transformation intention. In contrast to the net effect, the results of fsQCA show that different combinations of states of internal (human capital, organizational culture and technological capital) and external elements (institutional and market pressures) of the firm are likely to stimulate digital transformation intention.Originality/valueThis study provides empirically based insights into firms' digital transformation intentions and advances the current understanding of the drivers and inhibitors of digital transformation. Unlike most current research, which tends to focus on the net effect of factors influencing the digital transformation of enterprises, this study focuses on identifying the core elements influencing enterprises' digital transformation intention, especially the joint effect of different factors, both internal and external to the enterprise. The combined SEM and fsQCA findings of this paper not only enrich the existing theories on digital transformation but also have high value in guiding the digital transformation of firms.
Loss of organic cattle to the conventional value chains in Ireland: what are the economic and environmental impacts?
Authors: Jin Yan 
Published: September 2023

PurposeThis paper aims to quantify the loss (or leakage) of organic cattle to conventional value chains in Ireland and assess its economic and environmental impacts.Design/methodology/approachThe paper adopts a Bio-economy Input-Output (BIO) model, a quantitative economic model representing the interdependencies between different sectors of the economy, to assess the economic and environmental impacts of organic leakage in the Irish beef sector.FindingsThe study reveals that 17% of organic cattle aged under 1 year old leave the organic value chain, leaking to the conventional market as a result of imbalances in the development of the beef value chain. The economic cost of this organic leakage is 5.66 million euros. Leakage also has environmental effects because of changes in lifecycle methane and nitrogen emissions based on longer finishing times on organic farms and chemical fertilisers applied on conventional farms. The organic leakage results in a reduction of 82 tons of methane emission and 52 additional tons of nitrogen emission, which leads to 11,484 tons of net global warming potential (GWP) for a 100-year time horizon.Research limitations/implicationsBecause of data availability, the research focussed on the baseline year 2015, which had national data available for disaggregation in Ireland. Therefore, researchers are encouraged to assess the economic and environmental impacts when more recent data are available and to analyse the change in the impacts over the years.Practical implicationsThis study contributes to the discussion on organic conversion and provides valuable insights for stakeholders, especially policymakers, for the design of future organic schemes.Originality/valueThis is the first paper to assess organic leakage in the beef sector.
Decoupling institutional pressure: how and why academic journals do it
Authors: Silva Altieres de Oliveira , Avrichir Ilan 
Published: September 2023

Purpose
This study aims to verify empirically that when a group of isomorphic organisations is subjected to institutional pressure that conflicts with their technical efficiency or interests, this group will embrace opaqueness and decoupling.

Design/methodology/approach
This is a multiple case study of 16 Brazilian academic journals. The authors analyse whether the editorial boards of these journals play an effective or merely ceremonial role in the administration of the journals.

Findings
The authors find indications that isomorphic organisations revert to decoupling when the pressures they are subjected to are in conflict with their technical efficiency or interests. The authors also find indications of an inverted U-shaped relationship between the collaboration recruiting power of a journal in an academic field and decoupling. This collaboration recruiting power is closely related to the journal’s position in academic rankings.

Practical implications
The authors have shown that, although some scientific journals can deal with internationalisation pressures, for others, this is difficult and leads to decoupling and opaqueness. This is not a desirable situation. It can be counterproductive and draw attention to bureaucratic procedures.

Originality/value
This relation between opaqueness and institutional pressure for a group of organisations within the context of neo-institutional theory has not yet been verified empirically. This study’s results show how institutional pressure and organisational opaqueness are related in an organisational field. This theoretical contribution has practical implications because of decoupling’s potentially negative effects.
Making a digital and financial leap towards sustainable tourism in Ghana
Authors: Musah Alhassan , Yakubu Ibrahim Nandom , Shaibu Abdul-Fatawu 
Published: September 2023

PurposeThe study investigates the impact of information and communications technology (ICT) and financial development on tourism development in Ghana.Design/methodology/approachThe researchers employ data covering from 1995Q1 to 2020Q4 and apply the autoregressive distributed lag (ARDL) estimation technique.FindingsThe findings reveal that ICT exerts a positive significant impact on tourism development in both long- and short-term periods. The authors find that financial development has a negative significant effect on tourism development in the long run. However, financial development significantly increases tourism revenue in the short term. The results further reveal a significant positive link between infrastructure development and tourism receipts in the long run.Originality/valueThis study is a pioneering effort to investigate the impact of ICT and financial development on tourism development in Ghana, as far as the researchers are aware. Additionally, the use of an index of ICT adds novelty to the literature. In terms of policy, the findings of this study can inform policymakers on the importance of investing in ICT and financial development to boost the tourism industry in Ghana.
Paying attention in metaverse: an experiment on spatial attention allocation in extended reality shopping
Authors: Chen Juan , Xi Nannan , Pohjonen Vilma , Hamari Juho 
Published: September 2023

PurposeMetaverse, that is extended reality (XR)-based technologies such as augmented reality (AR) and virtual reality (VR), are increasingly believed to facilitate fundamental human practice in the future. One of the vanguards of this development has been the consumption domain, where the multi-modal and multi-sensory technology-mediated immersion is expected to enrich consumers' experience. However, it remains unclear whether these expectations have been warranted in reality and whether, rather than enhancing the experience, metaverse technologies inhibit the functioning and experience, such as cognitive functioning and experience.Design/methodology/approachThis study utilizes a 2 (VR: yes vs no) × 2 (AR: yes vs no) between-subjects laboratory experiment. A total of 159 student participants are randomly assigned to one condition — a brick-and-mortar store, a VR store, an AR store and an augmented virtuality (AV) store — to complete a typical shopping task. Four spatial attention indicators — visit shift, duration shift, visit variation and duration variation — are compared based on attention allocation data converted from head movements extracted from recorded videos during the experiments.FindingsThis study identifies three essential effects of XR technologies on consumers' spatial attention allocation: the inattention effect, acceleration effect and imbalance effect. Specifically, the inattention effect (the attentional visit shift from showcased products to the environmental periphery) appears when VR or AR technology is applied to virtualize the store and disappears when AR and VR are used together. The acceleration effect (the attentional duration shift from showcased products to the environmental periphery) exists in the VR store. Additionally, AR causes an imbalance effect (the attentional duration variation increases horizontally among the showcased products).Originality/valueThis study provides valuable empirical evidence of how VR and AR influence consumers' spatial bias in attention allocation, filling the research gap on cognitive function in the metaverse. This study also provides practical guidelines for retailers and XR designers and developers.
Innovative attributes, brand reputation and behavioral consequences in the clothing industry: the roles of self-congruity and value consciousness
Authors: Nik Hashim Nik Mohd Hazrul , Khalid Nor Rahimy , Mokhtaruddin Suraya Akmar , Al Mamun Abdullah , Razzaque Mohammed Abdur 
Published: September 2023

PurposeResearchers have paid little attention to elucidating how customer-perceived innovative apparel attributes are linked to brand reputation and consumer buying behaviors. This study intends to bridge that gap by providing empirical evidence on the effects of product novelty, product difference and product inimitability on brand reputation and behavioral intentions in the context of garment purchasing. We also investigate the moderating effects of self-congruity and value consciousness on the attribute‒brand reputation linkages, as well as their immediate influence on the domain variables.Design/methodology/approachThe proposed model was estimated using data from a web-based survey of 299 female apparel customers. Structural equation modeling was employed to test the relationships between variables.FindingsThe results indicate that product novelty, product inimitability, self-congruity and value consciousness significantly influence brand reputation. The results also demonstrate that self-congruity, value consciousness and brand reputation have direct effects on behavioral intention, while self-congruity and value consciousness appear to moderate the relationship between innovative product attributes and brand reputation.Originality/valueThis study is the first to present a conceptual model that systematically encompasses product innovation, brand perceptions and behavioral links in the field of women's clothing. The findings have important implications for both academics and practitioners in the field of fashion marketing.
The enhanced recovery after surgery program in elderly patients over 75 years of age undergoing elective colorectal cancer surgery
Authors: Hung Dorothy Sze Wing , Chu Colin Wai Ho , Kwok Kam Hung 
Published: September 2023

AbstractAimThe enhanced recovery after surgery (ERAS) program has been well established for patients undergoing colorectal surgery. However, there has been a lack of studies on its use for elderly patients. Our unit implemented the ERAS program in September 2018. The aim of this study is to evaluate the outcome of the ERAS program in patients over 75 years who are undergoing colorectal surgery.Patients and MethodsIn this retrospective study, we compared all patients aged 75 and above who were admitted to Queen Elizabeth Hospital for elective colorectal cancer surgery before the implementation of the ERAS program (1 January 2016 to 31 August 2018) with those treated after its implementation (1 April 2019 to March 2022). The primary end point was the post‐operative length of stay. The secondary end points were wound infection, retention of urine, pneumonia, deep vein thrombosis, pulmonary embolism, use of total parenteral nutrition, acute coronary syndrome, blood transfusion, reoperation, in‐hospital mortality, unplanned readmission within 28 days and Clinical Fragility Score.ResultsA total of 258 patients were included. The median length of stay was 8.4 days in the pre‐ERAS group vs 6.8 days in the ERAS group. In‐patient death (1.4% in the pre‐ERAS group vs 0.9% in the ERAS group) and readmission rate (7.7% in the pre‐ERAS group vs 12.2% in the ERAS group) were similar between the two groups. About 31.5% of patients in the pre‐ERAS group had higher Clinical Fragility Scale post‐operatively as compared with 7.8% in the ERAS group.ConclusionThe ERAS program shortened the length of hospital stay by 1.6 days, and patients had lower Clinical Fragility Scale post‐operatively. Adverse events were not increased. The ERAS program can be safely applied to elderly patients with colorectal cancer.
Comparative outcomes of hemodialysis patients facing pre‐Omicron and Omicron COVID‐19 epidemics
Authors: Beppu Hiroko , Fukuda Tatsuya , Otsubo Naoya , Akihisa Taro , Kawanishi Tomoko , Ogawa Toshie , Abe Yasutomo , Endo Mariko , Hanawa Tomohide , Sugita Chise , Kikkawa Yoshiaki , Yamada Tetsuya , Wakai Sachiko 
Published: September 2023

AbstractIntroductionThis study elucidates factors affecting the severity and mortality in pre‐Omicron and Omicron strains of SARS‐CoV‐2 and vaccination impact.MethodsThis single‐center retrospective observational study included 1598 hospitalized COVID‐19 patients. Patients were grouped into “pre‐Omicron” and “Omicron” periods. The endpoint was severe COVID‐19 (oxygen saturation [SpO2] < 94%). Logistic regression examined associations between clinical factors, including hemodialysis (HD), and the endpoint.ResultsThe HD patient mortality rate dropped from 16% pre‐Omicron to 4% during the Omicron epidemic. HD was significantly associated with the study endpoint in both epidemics. Unvaccinated patients had a greater risk of reaching the study endpoint among patients receiving HD.ConclusionThese findings suggest that the Omicron variant, alongside vaccination and healthcare innovations, led to improved prognoses for HD patients with COVID‐19. However, HD patients remain at a greater risk for severe COVID‐19. Increased vaccination rates and optimized healthcare resources can improve this vulnerable population's prognoses.
Verification of the efficacy of deciduae for determining the genetic type of mouse embryos
Authors: Imai Hiroyuki , Iwamori Naoki , Iwamori Tokuko , Matsuya Sumito , Kano Kiyoshi , Kusakabe Ken Takeshi 
Published: September 2023

AbstractMouse embryos in the early‐implantation stage require manipulation under a microscope. While the extraction of DNA, RNA and proteins from a single sample allows for both determination of genetic type and analysis of gene expression, whole mount analysis is not possible. In this study, we explored the applicability of PCR using extraembryonic tissues, especially the decidual side tissue after isolating the embryos from implantation sites to establish a method for determining the genetic type of embryos. The implantation site was resected at each day from the date of vaginal plug confirmation, separated into embryos and deciduae. Genomic DNA were isolated separately from the embryos and the deciduae. PCR was performed using these genomic DNA, and the band patterns were compared after electrophoresis. As a result, we demonstrated that detecting embryo‐derived cells in the decidua allows determination of the sex and presence of transgenes without harming the mouse embryos themselves, from 8.5 days of age. This method enables the determination of the genetic type of mouse embryos without damaging. This technique would expand the adaptations for analysis of mouse implanted embryos.
International responses addressing the under‐representation of older people in clinical research
Authors: Markham Susan C. , McNab Justin , O'Loughlin Kate , Clemson Lindy 
Published: September 2023

AbstractOlder people (aged 65 years +) are under‐represented in clinical research across many disease areas. Such exclusions limit the generalisability of trial results and can lead to a gap in critical knowledge regarding the efficacy and safety of interventions in older age groups. International bodies and regulators have addressed this issue to varying degrees. The USA's Food and Drug Administration (FDA) has produced regular non‐binding guidance about the inclusion of older trial participants, while the National Institutes of Health's (NIH) binding regulations mean that those seeking research funding must include participants of all ages unless there are scientific or ethical reasons to exclude them. European regulations governing clinical trials of new medications require that participants represent the population groups that are likely to use the product being tested, while Britain highlights the need to improve trial participation of under‐served groups, which include older people. Australian clinical trial guidelines point to the potential problem of excluding some groups from research but do not specifically address older participants. While current international approaches may provide some improvements in trial representation, additional strategies are required to promote clinical research that better reflects populations seen in clinical practice. Australia could benefit from clinical trial guidance that highlights the specific issue of the under‐representation of older participants and outlines strategies to facilitate greater inclusion. This article provides an overview and critique of the current approaches to the inclusion of older people in clinical research and highlights policy gaps and limitations of current strategies.