bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Characterisation of Human Respiratory Adeno Viruses in Uganda
Authors: Ukuli Qouilazoni Aquino , Erima Bernard , Mubiru Andrew , Atim Gladys , Tugume Titus , Kibuuka Hannah , Mworozi Edison , Ducatez Mariette F. , Wabwire-Mangeni Fred , Byarugaba Denis K. 
Published: December 2022

Abstract
Human adenoviruses (HAdV) are a diverse group of viruses causing a broad range of infections of the respiratory, urogenital and gastrointestinal tracts, and keratoconjunctivitis. There are seven species of human adenoviruses with over 51 serotypes and 69 genotypes which may contain multiple genetic variants. This study characterised the genotypes of human respiratory adenoviruses and associated factors in samples collected from selected hospitals in Uganda. A total of 2,298 nasopharyngeal samples were collected from patients seeking health care at tertiary hospitals for influenza-like illness. They were screened by Polymerase Chain Reaction (PCR) to determine the prevalence of HAdV. HAdV was isolated in A549 cell lines and the Hexon gene from the HAdV isolates was sequenced by sanger sequencing method using the BigDye Terminator v3.1. Of the 2,298 samples tested, 225 (9.8%) were adenovirus positive by PCR. Age and HAdV infection were found to be significantly associated (p = 0.028) with 98% (220/225) of the positives in children below 6 years and none in adults above 25 years of age. The sequenced isolates belonged to species HAdV B and HAdV C with most isolates identified as genotype B3. The results showed a high prevalence and HAdV genetic diversity in respiratory HAdV circulating in Ugandan population. Children aged between 0 to 5 years were more likely to get infected with HAdV than other age groups. Deeper genomic characterization based on whole genome sequencing is necessary to further elucidate possible transmission and impact of current adeno-vectored vaccines in Africa.
Comparison of multimodel ensembles of global and regional climate models projections for extreme precipitation over four major river basins in southern Africa. Part II: Future changes under 1.5°C, 2.0°C and 3.0°C warming levels
Authors: Samuel Sydney , Dosio Alessandro , Mphale Kgakgamatso , Faka Dieudonne Nsadisa , ni Modise 
Published: December 2022

AbstractWe compare the consistency of the future response of extreme precipitation to 1.5°C, 2.0°C, and 3.0°C global warming levels (GWLs) above the pre-industrial period (1850–1900) over four major basins in southern Africa. In particular, we compare the results from large ensembles of global climate models (GCMs) and regional climate models (RCMs) from the Coupled Model Intercomparison Project (CMIP5 and CMIP6) and the COordinated Regional-climate Downscaling EXperiment (CORDEX and CORE). The results show that the signal of the changes under all GWLs is generally consistent across the ensembles, particularly for the spatially averaged medians over each basin. Generally, all the ensembles project an increase in maximum consecutive dry days (CDD) and simple daily rainfall intensity (SDII) and a decrease in maximum consecutive wet days (CWD) and the total number of rainy days (RR1) over the basins under all GWLs. However, there are differences in the signal and robustness of the projected changes among the ensembles. For instance, CORDEX projects a robust increase in heavy precipitation days over most of the Zambezi basin under 2.0°C and 3.0°C GWLs, while CORE projects a robust decrease over most of the basins. Additionally, the projected decrease in RR1 is robust over most parts of the basins in CMIP5, CORDEX, and CORE under all GWLs, but uncertain over most parts in CMIP6. Overall, CORE projects larger areas of robust changes in extreme precipitation compared to other ensembles, with uncertain changes over most parts of the basins in CMIP6. For the avoided impacts by limiting global warming at 1.5°C, all the ensembles agree that the impacts of extreme precipitation will be reduced in general over basins under 1.5°C GWLs compared to 2.0°C and 3.0°C GWLs.
Thyroid function in opium users: A Cross-Sectional Study on PERSIAN Cohort of Fasa, 2017
Authors: Pezeshki Babak , Pourmontaseri Hossein , Homayounfar Reza , Moghaddam Maryam Talebi , Dehghan Azizallah 
Published: December 2022

Abstract
Background
Addiction increases the risk of different lifelong disorders. However, there are limited studies evaluating the effects of opium use on thyroid. The present study aimed to compare the thyroid function of opium users and non-users.
Methods
This cross-sectional study was conducted on 700 participants of the Persian Cohort of Fasa, Iran. Pregnant women and participants with false or missing data were excluded from the study. Frozen plasma samples of the cohort bank were used to determine the levels of T3, T4, and thyroid-stimulating hormone (TSH). The thyroid function was compared between the two groups using Mann-Whitney test (P < 0.05).
Results
The mean age of the study population (n = 648) was 54.0 ± 9.8 years, including 336 men (49.1%) and 197 opium users (28.8%). The median levels of TSH, T4, and T3 were 2.91 ± 4.61, 9.26 ± 3.65, and 1.22 ± 0.49, respectively. The opium users had significantly higher TSH (3.72 ± 6.2 vs. 2.58 ± 3.75) and lower T4 (8 ± 3.6 vs. 9.8 ± 3.5) levels (P < 0.001). Also, T3 was slightly lower in opium users (1.1 ± 0.5 vs. 1.3 ± 0.5; P = 0.369), although this association was only significant in female opium users (P < 0.001).
Conclusions
The present findings revealed that opium use causes a reduction in T4, while increasing TSH. Therefore, opium use may lead to the development of primary hypothyroidism, which needs to be investigated in future studies.
Uncovering the relationship between YTHDF1 related to immune cells and prognosis and diagnosis in lung adenocarcinoma
Authors: Han Lei , Liu Yan , Wu Jia , Fan Bin , Wang Lei , Li Genshan , Yao Zhihong , Ye Lijuan , Li Dongqi , Yang Zuozhang 
Published: December 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Characterization of the Complete Mitochondrial Genome of Cucurbita maxima Duch
Authors: Liu Zefa , Jiang Yanfang , Zhu Hongye , Gong Yihui , Yusop Mohd Rafii , Abdullah Shamsiah 
Published: December 2022

Abstract
Cucurbita maxima Duch is a widely cultivated and consumed vegetable with high economic and nutritional value. The complete mitochondrial (mt) genome of C. maxima was characterized by Illumina paired-end sequencing and is reported here. The size of the complete mt genome was 640,814 bp, and its GC content was 43.37%. Sequence analyses showed that the mt genome encoded 68 genes, consisting of 38 mRNA, 27 tRNA, and 3 rRNA genes. Phylogenetic analysis indicated that C. maxima was most closely related to Cucurbita pepo (NC-014050.1). This study provides useful genetic information for further studies on the genetic structure and evolutionary patterns of C. maxima for species identifications. The genome can be accessed on GenBank with the accession number (OL350846.1).
Identifying factors that affect the use of health information technology in the treatment and management of hypertension
Authors: Faezi Aysan , Afshar Hadi lotfnezhad , Rahimi Behlol 
Published: December 2022

Abstract
Background: We conducted this study with the aim of Identifying factors that affect the use of health information technology in the treatment and management of hypertension.
Methods: This is a descriptive-analytic study conducted in 2022. To obtain relevant articles, databases including Scopus, Web of Science, IEEE, and PubMed were searched and the time period was between 2013 and 2022. Based on the review of similar articles, a five-point Likert scale checklist was developed in the second phase. The statistical population of the present study was specialist physicians (n=40) and patients (n=384). In order to analyze the data, SPSS Statistics 24 was used. We used descriptive measures to show the data on the two affecting categories on the checklist, followed by a One-Way ANOVA.
Results: As a result of the review literature process, 50 papers were screened, that based we can distinguish motivational and inhibitory factors affecting the use of health information technology in hypertension management. Indeed, Motivational factors and inhibitory factors can be classified into five groups: organizational, economic, technical, personal, and legal/moral factors. Based on the results of the survey, the factors that were identified as most influential on patient and specialist physicians' motivation and inhibitory to use of health information technology to manage and treat hypertension.
Conclusion: Utilizing technologies for hypertension management can be improved by identifying motivating and inhibiting factors. This approach can improve the acceptability of these technologies, save costs, reduce long-term complications of hypertension, and improve patient quality of life.
Psychometric properties of a standardized protocol of muscle strength assessment by hand-held dynamometry in healthy adults: A reliability study
Authors: Morin Marika , Hébert Luc J. , perron Marc , Petitclerc Émilie , Lake Shanna-Rose , Duchesne Elise 
Published: December 2022

Abstract

Background Maximal isometric muscle strength (MIMS) assessment is a key component of physiotherapists’ work. Hand-held dynamometry (HHD) is a simple and quick method to obtain quantified MIMS values that have been shown to be valid, reliable, and more responsive than manual muscle testing. However, the lack of MIMS reference values for several muscle groups in healthy adults with well-known psychometric properties limits the use and the interpretation of these measures obtained with HHD in clinic. Objective To determine the intra- and inter-rater reliability, standard error of measurement (SEM) and minimal detectable change (MDC) of MIMS torque values obtained with HHD. Methods Intra and Inter-rater Reliability Study. The MIMS torque of 17 muscle groups was assessed by two independent raters at three different times in 30 healthy adults using a standardized HHD protocol using the MEDup™ (Atlas Medic, Québec, Canada). Participants were excluded if they presented any of the following criteria: 1) participation in sport at a competitive level; 2) degenerative or neuromusculoskeletal disease that could affect torque measurements; 3) traumatic experience or disease in the previous years that could affect their muscle function; and 4) use of medication that could impact muscle strength (e.g., muscle relaxants, analgesics, opioids) at the time of the evaluation. Intra- and inter-rater reliability were determined using two-way mixed (intra) and random effects (inter) absolute agreement intraclass correlation coefficients (ICC: 95% confidence interval). SEM and MDC were calculated from these data. Results Intra- and inter-rater reliability were excellent with ICC (95% confidence interval) varying from 0.90 to 0.99 (0.85–0.99) and 0.89 to 0.99 (0.55–0.995), respectively. Absolute SEM and MDC for intra-rater reliability ranged from 0.14 to 3.20 Nm and 0.38 to 8.87 Nm, respectively, and from 0.17 to 5.80 Nm and 0.47 to 16.06 Nm for inter-rater reliability, respectively. Conclusions The excellent reliability obtained in this study suggest that the use of such a standardized HHD protocol is a method of choice for MIMS torque measurements in both clinical and research settings. And the identification of the now known metrological qualities of such a protocol should encourage and promote the optimal use of manual dynamometry.
Evaluation of SPECT imaging: a multi-center and multi-vendor phantom study
Authors: Gonçalves Maria Inês Rebelo , Parafita Rui , Costa Mauro , Geão Ana , Pereira Edgar , Vieira Lina 
Published: December 2022

Abstract
Background: Single-photon emission computed tomography (SPECT) is a nuclear medicine (NM) imaging method sensitive for the evaluation and quantification of physiological processes in patients. However, there are different factors that affect qualitatively and quantitatively the SPECT images and, consequently, the reliability of the data. There is a degree of variability underlying the intrinsic performance of systems from the same or different manufacturers. In addition, the different configurations of the gamma cameras (GC) and the acquisition and reconstruction protocols used may lead to heterogeneity of results. This study compared SPECT images acquired in three GC from three NM centers, using the Jaszczak and NEMA IEC Body phantoms, filled with homogeneous solution of technetium-99m. Acquisitions were performed in each GC, following the protocols used in clinical practice of the respective NM center, and the images were analyzed and compared in terms of spatial resolution, sensitivity, noise, contrast, and contrast recovery (CR). Subsequently, the processing and reconstruction parameters were harmonized to assess their impact on the standardization of results.
Results: With the clinical protocols, the images acquired with the three GC showed small differences in spatial resolution, noise, and contrast, with the greatest difference being observed in the quantitative accuracy, since the CR has achieved variabilities of 0.17 and 0.28, for different activity concentrations. This intersystem variability was reduced to 0.09 and 0.08 after reconstruction algorithm standardization. However, this change increased the differences in the contrast percentage.
Conclusion: GC from different manufacturers showed the greatest heterogeneity of results, however, by eliminating possible sources of variation it is possible to reduce the differences between systems. It was demonstrated the need to include different parameters for the evaluation and comparison of SPECT images, so that procedures can be established between the different NM centers to ensure a trade-off between the various parameters assessed here, as the improvement of some usually leads to the deterioration of others.
Significance of upper paratracheal lymph node resection in stage IB right lung cancer
Authors: Wang Feng , Yu Xiangyang , Han Yi , Zhang Lanjun , Liu Shuku 
Published: December 2022

Abstract
Objective
The aim of this study was to investigate the effect of upper paratracheal lymph node (2R lymph node) resection on the prognosis of stage IB right lung cancer
Methods
We retrospectively collected data of 339 patients with stage IB right lung cancer who underwent radical surgery from 1999 to 2009. The prognostic factors were evaluated by Cox proportional hazard regression model. Propensity score matching (PSM) was applied to adjust the confounding factors. The Kaplan-Meier method was applied to evaluate overall survival (OS) and recurrence-free survival (RFS).
Results
For all 339 cases included in the study, Univariate and multivariate analysis showed that smoking and family history of malignant tumors were prognostic factors for OS. Age and family history of malignant tumors were prognostic factors for RFS. Resection of the 2R lymph node was not statistically associated with OS and RFS. All cases were separated into two groups based on whether the 2R lymph nodes were resected. The results of survival analysis after PSM also suggest that the resection of 2R lymph node is not statistically associated with OS and RFS (P > 0.05).
Conclusions
For stage IB right lung cancer, upper paratracheal lymph node resection was not statistically associated with OS or RFS. 2R lymph node resection may not be necessary for early-stage NSCLC.
A prior knowledge-informed traceableNeutral Network modeling only using regular laboratory results to assist early diagnosis for tuberculosis: a multiple-center study in China
Authors: LIANG Yu-fang , Zheng Hua-rong , Huang Da-wei , Nai Jing , Wang Yan , Cui Wei-qun , Feng Li-na , Li Xu-sheng , Fan Meng-guang , Luo Yi-fei , Chen Chao , Wang Qing-tao , Zhou Rui 
Published: December 2022

AbstractBackground:To construct a knowledge-informed traceable artificial intelligence (AI)-based model to assist early diagnosis for tuberculosis (TB).Methods:60729 cases were extracted from January 1, 2014, to December 31, 2021, in Beijing Hepingli Hospital. Beijng Jishuitan Hospital was used as an independently external testing set. Only using routine laboratory results, six models based on Neutral Network (NN) algorithm combined with clinical prior knowledge were designed for TB screening and differentials were set up. Our TB model was not only quantitatively evaluated by means of metrology, but also validated by an independently external testing set from Beijing Jishuitan Hospital, and by on-site clinical validation in 37 hospitals.Results:For disease screening, our NN algorithm overall performed better than the other algorithms for diseases & healthy control (HC), and TB & non-TB models. Taking an example for the TB& non-TB model, the AUC, ACC, SPE and SEN were 0.9240, 0.7703, 0.7664 and 0.8958 respectively. For disease differentials, The AUC was 0.8035 for pulmonary tuberculosis (PTB) & other pulmonary diseases (OPD) model; the AUC was 0.7761 for tuberculosis（TB）& extrapulmonary tuberculosis（EPTB）model. For an on-site clinical validation in Baoding No.2 Central Hospital, the average accuracy was stable, achieving 93% for TB& non-TB model.Conclusions:A knowledge-informed AI-based model only based on regular laboratory results offers a more convenient, effective, and highly accurate early diagnosis tool for TB.
Analysis of remote sensing monitoring of atmospheric ozone in Japan for the past 12 years
Authors: Lei Shengtong , Ju Tianzhen , Li Bingnan , Xia Xuhui , Huang Cheng , Zhang Jiaming , Li Chunxue 
Published: December 2022

Abstract
It is ozone pollution great significance to the study of photochemical smog to understand the present situation of ozone pollution in Japan and provide theoretical basis for solving ozone pollution problem. Based on remote sensing data from OMI satellites, MK and Slope trend analysis, Hurst index analysis, the potential source contribution factor algorithm, Kendall correlation analysis, and the random forest regression model were used in this paper to investigate ozone column concentrations in Japan from 2010 through 2021. The results showed that ozone column concentration have shown a significant latitudinal trend over the past 12 years, ozone column concentration have increased with latitude, the spatial distribution patterns have remained consistent from month to month, and the slope has showen a lower upward trend over the years (slope = 0.0052). The interannual maximum and minimum ozone column concentrations are in 2021 (387.57 DU) and 2015 (241.27 DU), respectively. The monthly maximum and minimum values occur in February (443.03 DU) and December (234.04 DU). Spring (352.94 DU) > winter (336.01 DU) > summer (306.78 DU) > autumn (296.30 DU). Column ozone concentrations have increased in 69.82% of Japan over the past 12 years. In 2022, the maximum, minimum, and mean ozone column concentrations based on random forest forecasts are 372DU, 278DU, and 334DU, respectively. pollution sources in Japan are largely derived from pollutants that are transported across borders from the seas around the country or from other countries in the world, with the largest potential source areas being located in the Kanto region. Ozone is negatively correlated with precipitable water and air temperature, positively correlated with nitrogen dioxide and the rising index, and uncorrelated with air pressure. The contribution of population and GDP to ozone is small.
The construction and analysis of ceRNA and tumor-infiltrating immune cell networks in the distant metastasis of lung adenocarcinoma
Authors: liu Jun , zhu Yun , Guo Yuntao , Tang Shuainan , Fu Guolong , Zhang Tongsong , Song Haiping , Zhao Dezhi , Wang Licheng , Jiang Xin 
Published: December 2022

AbstractLung adenocarcinoma (LUAD) is the most common lung cancer with a high rate of distant metastasis. The potential mechanism of competitive endogenous RNA (ceRNA) and tumor-infiltrating immune cells in LUAD distant metastasis is still unclear. RNA profiles of LUAD were downloaded from the cancer genome atlas (TCGA) including primary LUADs without or with distant metastasis. The miRNA-mRNA and lncRNA-miRNA interaction information were downloaded to establish the distant metastasis-specific ceRNA network. The algorithm “cell type identification by estimating relative subsets of RNA transcripts (CIBERSORT)” was used to estimate their immune cells. The Pearson correlation analysis was utilized to compare the prognostic biomarker in the ceRNA network and immune cell proportion. We established the two nomograms of metastasis-related ceRNA networks and immune cells. The Receiver Operating Characteristic (ROC) and the calibration curves indicated acceptable accuracy and discrimination of the nomogram. Based on the correction analysis between metastasis-related ceRNA and immune cells, along with external validation, NR3C2, PIK3R1, PTPN13, SPRY2 and mast cells resting were found to be associated with each other and constitute the potential regulation networks. Our data identify prognostic ceRNAs and immune cells and provide two prediction nomograms. Besides, the potential regulatory networks among NR3C2, PIK3R1, PTPN13, SPRY2 and mast cells resting are also uncovered, which may take part in the distant metastasis of LUAD.
Diversity under a magnifier lens: The case of Typhlotanaidae in N Atlantic
Authors: Gellert Marta , Błażewicz Magdalena , Mamos Tomasz , Bird Graham J. 
Published: December 2022

Abstract
Research focusing on ‘stout-bodied’ typhlotanaids collected from several sites around Iceland and adjacent northern seas has resulted in the description of 15 species new to science, as well as the creation of eight new genera. Typhlotanais eximius Hansen, 1913 was redescribed and transferred to a new genus, while Typhlotanais crassus and Peraeospinosus adipatus are transferred to the genus Larsenotanais. The morphological and the molecular data were combined to consolidate and confirm the validity of the results obtained from both approaches. The polyphyletic nature of Typhlotanaidae and its serious underestimation was emphasized. Molecular analysis revealed that the ’stout-bodied‘ Typhlotanaidae are monophyletic. Depth, temperature, and salinity were identified as the main environmental parameters determining the distribution of this group of Typhlotanaidae. Several species were clearly associated with shelf and upper bathyal of Iceland. The Greenland-Iceland-Faroe ridge is shown to be a distinct zoogeographical barrier for typhlotanaids inhabiting the deeper slope and abyssal around Iceland.