bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implications of an antagonist age for maritime trade and its impacts on energy demand
Authors: Franz Sebastian , Kramel Diogo , Strømman Anders , Bramstoft Rasmus , Muri Helene , Münster Marie 
Published: April 2023

Abstract
In a time where high geopolitical tensions are affecting global trade patterns, we step back and challenge the common assumptions of low rivalry and perfect markets among nations in energy system modeling. We developed a gravity demand model to evaluate how antagonism, characterized by reduced globalization and rivalrous nation-to-nation relationships, impacts maritime trade patterns and energy demand levels needed to support the movement of trade goods. Our model enables maritime trade and energy demand projections to vary with socioeconomic and geopolitical futures. The results show that increasing antagonism between nations leads to less energy-efficient routes and longer distances to cover. On average, these impacts increase annual maritime fuel demands by 13% or 1,500PJ (110 MtonCO2eq in today's fuel mix). Thus, geopolitical tension exacerbates maritime climate mitigation challenges and therefore highlights the need to carefully shape this hard-to-abate sector's future to not increase emissions further.
RBM39 is a potential prognostic biomarker associated with immune infiltrates in hepatocellular carcinoma
Authors: Cui Fangfang , Wang Wenling , Zhuang Chunbo , Wang Pei 
Published: April 2023

Abstract
RNA-binding motif protein 39 (RBM39) is a well-studied RNA-binding protein that has been reported to be associated with the process of tumorigenesis and the development of numerous cancers. However, its role in hepatocellular carcinoma (HCC) remains unclear. This study aimed to understand the prognostic value of RBM39 in HCC by investigating the relationship between RBM39 expression and clinicopathological features. The cancer genome atlas (TCGA) and gene expression omnibus (GEO) databases were used to analyze the differential expression of RBM39 between normal tissues and that of HCC. Kaplan–Meier and COX regression models were used to analyze the correlation between RBM39 expression and survival rate in the case of HCC. Moreover, gene set enrichment analysis (GSEA) was performed to identify key pathways associated with RBM39. The correlation of RBM39 with tumor immune infiltration was evaluated by single sample gene set enrichment analysis (ssGSEA) using TCGA data. The data generated by bioinformatic approach were further validated using qRT-PCR and immunohistochemistry. The CCK8 and Wound healing assays were performed to investigate the biological function of RBM39 in HCC cells. Our results indicated that there was a significant upregulation of RBM39 in HCC as compared to that of normal tissues. High RBM39 expression was significantly associated with advanced T-stage, histological grade, and pathological stage, and predicted poor overall survival (OS), disease-free survival (DSS), and progression-free interval (PFI) in HCC patients. Multivariate Cox analysis further confirmed that the upregulation of RBM39 expression was an independent prognostic factor for OS in HCC. Moreover, GSEA enrichment analysis indicated that RBM39 was functionally involved in pathways associated with cell cycle, DNA replication, P53, and primary immunodeficiency. RBM39 expression was associated with the infiltrating levels of Th2 cells and DC cells. Knockdown of RBM39 significantly inhibited the proliferation and migration of HCC cells. Altogether, these findings suggest an important role of RBM39 in the development, diagnosis, and prognosis of HCC.
Signature identification based on immunogenic cell death-related lncRNAs to predict the prognosis and immune activity of patients with endometrial carcinoma
Authors: Yao Yuwei , Zhang Qi , Wei Sitian , Li Haojia , Zhang Jiarui , Zhang Jun , Wang Hongbo 
Published: April 2023

Abstract
Background: Endometrial carcinoma (EC) is one of the most prevalent gynecologic malignancies and requires further classification for treatment and prognosis. Long non-coding RNAs (lncRNAs) and immunogenic cell death play a critical role in tumor progression. Nevertheless, the role of lncRNAs in immunogenic cell death in EC remains unclear. This study aimed to explore the role of immunogenic cell death (ICD) related-lncRNAs in EC via bioinformatics and establish a prognostic risk model based on the ICD related-lncRNAs. We also explored immune infiltration and immune cell function across prognostic groups and made treatment recommendations.
Methods: A total of 552 EC samples and clinical data of 548 EC patients were extracted from The Cancer Genome Atlas (TCGA) database and UCSC Xena, respectively. A prognostic-related feature and risk model were developed using the least absolute shrinkage and selection operator (LASSO). Subtypes were classified with consensus cluster analysis and validated with t-Distributed Stochastic Neighbor Embedding (tSNE). Kaplan-Meier analysis was conducted to assess differences in survival. Infiltration by immune cells was estimated by ssGSEA, TIMER algorithm. By using the "pRRophetic" R package, the IC50 of drugs was compared among risk subgroups. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) was performed to verify the expression of lncRNA in clinical EC tissues and cell lines.
Result: In total, 16 immunogenic cell death-related lncRNAs with prognostic values were identified. Using SCARNA9, FAM198B-AS1, FKBP14-AS1, FBXO30-DT, LINC01943, and AL161431.1 as risk model, their predictive accuracy and discrimination were assessed. We divided EC patients into high-risk and low-risk groups based on the risk model. The analysis showed that the risk model was an independent prognostic factor. The prognosis of the high- and low-risk groups was different, and the overall survival (OS) of the high-risk group was lower. The low-risk group had higher immune cell infiltration and immune scores. Consensus clustering analysis divided the samples into four subtypes, of which cluster 4 had higher immune cell infiltration and immune scores.
Conclusion:A prognostic signature composed of 6 immunogenic cell death related-lncRNAs in EC was established, and a risk model based on this signature can be used to predict the prognosis of patients with EC.
Integrated analysis of messenger RNA and microRNA in patients with community- acquired pneumonia-associated sepsis
Authors: Oda Sayaka , Matsumoto Hisatake , Togami Yuki , Yoshimura Jumpei , Ito Hiroshi , Onishi Shinya , Muratsu Arisa , Mitsuyama Yumi , Okuzaki Daisuke , Ogura Hiroshi , Tanaka Susumu , Oda Jun 
Published: April 2023

AbstractBackground:Community-acquired pneumonia (CAP) is defined as an acute lung infection involving the alveoli that occurs in a patient without recent health care exposure. A complication of CAP is severe sepsis, a syndrome of infection often accompanied by systemic inflammation and organ dysfunction. The aim of this study was to evaluate mRNA and miRNA in whole blood and to perform an integrative analysis to assess cellular signals that play a role in the pathogenesis of patients with CAP-associated sepsis.Methods:This was a prospective, observational, single-center study of patients transported to the Department of Traumatology and Acute Critical Medicine, Graduate School of Medicine, Osaka University. Patients with CAP-associated sepsis were analyzed. The diagnosis of pneumonia was made according to the clinical findings, including blood samples and chest computed tomography scan, and the diagnosis of sepsis followed the Sepsis-3 guidelines.Results:We included 14 critically ill patients with CAP-associated sepsis and 15 healthy control subjects (HCS). The median ages of the patient group and HCS were 78 and 55 years, and their body mass indexes were 22.8 and 21.7 kg/m2, respectively. All patients were treated at the critical care center, and 11 of the 14 patients received ventilatory management. All patients survived. These 14 patients met the diagnostic criteria of Sepsis-3 and were diagnosed as having CAP-associated sepsis. Of them, 6 patients met the diagnostic criteria for septic shock. RNA sequencing showed the number of genes with up:down (upregulated:downregulated) expression variation (false discovery rate [FDR] <0.05, |log2 fold change| >1.2) to be 1209:1461 for mRNA; 51:21 for microRNA; and 646:1274 for miRNA-targeted mRNA. Canonical pathway analysis using mRNA showed activation of the PD-1 and PD-L1 cancer immunotherapy signaling pathways and inhibition of the Th1 signaling pathway as well as that using miRNA-targeted mRNAs.Conclusions:Using integrated analysis of mRNA and miRNA, we elucidated for the first time, to our knowledge, that T-cell exhaustion occurred during the acute phase of CAP-associated sepsis and that miRNA regulated Th1 signaling and PD-1 and PD-L1 cancer immunotherapy signaling through the RNA interference action of mRNA.
Clinical Analysis of Febrile Seizures in Children with COVID-19 in the Omicron Era: a Single-Center Retrospective Observational Study
Authors: Liu Ling , Ou Rong , Tan Zhong-You , Wang Ning , Chen Hongxia 
Published: April 2023

Abstract
Objectives：This study sought to investigate the characteristics of febrile seizures in children infected with the Omicron variant in Chongqing province, west of China, and underscore the importance of monitoring for potential neurological complications associated with this variant.
Methods: This retrospective study enrolled a total of 84 pediatric patients with COVID-19 and FS who were admitted to Chongqing University Three Gorges Hospital between December 11th and December 26th, 2022. Demographic, clinical, laboratory, radiological and EEG data were retrospectively summarized.
Results: The study enrolled 84 children, with a median age of 21.5 (15-35.5) months and a range of 6-162 months. Among these, 11.9% were of atypical age (age > 5 years). The patient population comprised of 54 (64.29%) boys and 30 (35.71%) girls. 32.14% presented with complex FS. Generalized tonic-clonic seizures occurred in 51.19%, followed by generalized tonic seizures (43.43%). 86.9% occurred within 24h after fever onset and 80.95% continued for ≤ 5min.
Conclusions: Febrile seizures in children with Omicron VOC are common COVID-19 illness with a higher prevalence compared with other VOCs. They present with similar clinical manifestations and resolve spontaneously with a benign clinical outcome in line with other seasonal viruses.
DETrack: Multi-object tracking algorithm based on feature decomposition and feature enhancement
Authors: Wen Feng , Huang Haixin , Hu Xiaojie , Yin Xiangyang , Ma Junguang 
Published: April 2023

Abstract
Multi-object tracking (MOT) algorithms are typically classified as one-shot or two-step algorithms. The one-shot MOT algorithm is widely studied and applied due to its fast inference speed. However, one-shot algorithms include two sub-tasks of detection and re-ID, which have conflicting directions for model optimization, thus limiting tracking performance. Additionally, MOT algorithms often suffer from serious ID switching issues, which can negatively affect the tracking effect. To address these challenges, this study proposes the DETrack algorithm, which consists of feature decomposition and feature enhancement modules. The feature decomposition module can effectively exploit the differences and correlations of different tasks to solve the conflict problem. Moreover, it can effectively mitigate the competition between the detection and re-ID tasks, while simultaneously enhancing their cooperation. The feature enhancement module can improve feature quality and alleviate the problem of target ID switching. Experimental results demonstrate that DETrack has achieved improvements in multi-object tracking performance, while reducing the number of ID switching. The designed method of feature decomposition and feature enhancement can significantly enhance target tracking effectiveness.
Factors affecting vocational choice among senior nursing students: A cross-sectional study
Authors: Lee Myung Kyung , Oh Jihyun 
Published: April 2023

Abstract
Background: During clinical placement, nursing students may experience unease and moral distress, negatively impacting their professional values and vocational choices.This study explored factors affecting nursing students’ vocational choices.
Methods: This cross-sectional study recruited the participants from three universities in South Korea Between September and October 2021. This study was adopted with a convenience sample of 270 Korean fourth-year nursing students who completed their clinical practice at tertiary general hospitals in three provinces in the North, Middle, and South areas of South Korea. Data were collected through a self-administered structured questionnaire.
Results: The factors affecting higher vocational choice were higher professional values, intention not to choose nursing as a future career, having no vision for choosing nursing, democratic family climate, and having at least one parent who was a medical professional.
Conclusion: Fostering students’ nursing professionalism and professional values through university education curricula may help maintain nursing students’ professional identity.
AdultCarbQuiz for the portuguese population with type 1 diabetes mellitus: translation, cultural adaptation and validation of its metabolic impact
Authors: Lopes Valentim , Peixoto Ana , Lages Adriana De Sousa 
Published: April 2023

Abstract
Background: In type 1 diabetes mellitus (T1DM), functional insulin therapy, based on carbohydrate (CH) counting and individualized insulin-to-carbohydrate ratio, is essential to achieve an adequate metabolic control. However, to date, few tools have been validated to assess patients' knowledge about CH counting, with the AdultCarbQuizquestionnaire having proved to be a reliable method in an American cohort. The aim of this study was, therefore, to translate, culturally adapt and validate the AdultCarbQuiz questionnaire for the Portuguese population.
Methods: Cross-sectional study of patients with T1DM on functional insulin therapy through continuous subcutaneous insulin infusion (CSII) followed in the Endocrinology department of a tertiary center. Prior to its application, the AdultCarbQuiz questionnaire was translated and culturally adapted to the Portuguese context. Statistical analysis was performed using the SPSS® software, v.27, with p values < 0.05 being considered significant.
Results: 100 patients were included, 58% of female sex, with a mean age of 31.09 ± 10.77 years. Mean disease duration was 15.04 ± 9.23 years and mean CSII usage time was 4.02 ± 3.90 years. The average value of glycated haemoglobin (HbA1c), Time In Range (TIR), Time Above Range (TAR) and Time Below Range (TBR) was, respectively, 7.32 ± 0.87, 59.75 ± 14.13, 34.38 ± 15.40 and 5.75 ± 6.58%. The average score of the questionnaire was 30.86 points ± 3.58 points, considered high. The Kuder-Richardson 20 coefficient value was 0.63 for the total score, with a Spearman-Brown value for the half-split of 0.63. Individuals with lower HbA1c values scored significantly higher on knowledge about hypoglycemia prevention and correction (r = - 0.269, p = 0.007) and on the total questionnaire score (r = - 0.205, p = 0.041). A higher TIR and a lower TAR were also associated with a higher total score (r = 0.274, p = 0.007 and r = - 0.274, p = 0.007, respectively).
Conclusion: In this study, the AdultCarbQuizquestionnaire, translated and culturally adapted to the Portuguese context, proved to be a useful tool in assessing knowledge about CH counting in patients with T1DM, allowing to optimize, individually, the therapeutic strategy in consultation.
U-shaped Loop：A Strain Relief Loop for Preventing Vagal Nerve Stimulator Lead Fracture
Authors: Mei Tao , Chen Yong , Shi Xin 
Published: April 2023

Abstract
Background: Vagal nerve electrical stimulation (VNS) is one of the effective neuromodulatory therapies for drug resistant epilepsy (DRE). Common device complications include lead fracture, device failure or battery dislodgement, resulting in various clinical symptoms. We report a case of lead fracture after VNS surgery, which provides inspiration during VNS surgery.
Case presentation: The patient was a 10-year-old child treated with VNS for DRE. The child's body was still growing, and after surgery, he occasionally engaged in physical activity, which led to an increase in the number of seizures after a neck rotation. An examination of the VNS device was performed, which showed that the wire lead was broken. The patient decided to have a VNS replacement surgery. During the operation, we maintain a certain degree of relaxation of the lead through two U-shaped loops as strain relief loops in the neck to prevent the lead fracture during neck activity. Postoperative epilepsy was well controlled, and no more seizures occurred during physical activity and neck rotation.
Conclusions: Maintaining a certain degree of relaxation of the lead in the neck during VNS operation can reduce the occurrence of lead fracture caused by the patient's body growth and neck rotation after surgery.
Evolutionary analysis of TCP gene family and its response to hormonal and abiotic stress in rye (Secale cereale L.)
Authors: Ren Yanyan , Ma Rui , Xie Mu hua , Lian Qinggui , Jiang Tingting 
Published: April 2023

Abstract
Background Teosinte branched1/cycloidea/proliferating cell factor (TCP) proteins are plant-specific transcription factors involved in diverse biological processes, including growth and development, abiotic stress resistance, leaf senescence, lateral branch formation, and pollen development. However, the evolution of the TCP family in the rye is poorly understood.Results A total of 26 TCP genes were identified on seven rye chromosomes. The genes were divided into three subfamilies based on the homology analysis of Arabidopsis thaliana, T. aestivum, H. vulgare, O. sativa, Z. mays, and A. tauschii (not identified) were selected as the related species of rye to evaluate the developmental mechanism of ScTCP gene family during the evolutionary process via motif and collinearity analyses. The results showed that fragment replication events modulated ScTCP gene family expansion. In addition, interactions among ScTCP proteins and the identification of promoters containing many hormonal and stress-responsive cis-acting elements suggested that the regulation of ScTCP expression is complex. The expression patterns of 12 genes were analyzed under different tissues, fruit development stages, three hormones, and six abiotic stresses.Conclusions The results demonstrated that ScTCP genes, especially ScTCP6, play a critical role in rye development and its response to various hormones and abiotic stresses. This study provides new insights into the evolution of TCP genes in rye and thus may promote molecular breeding.
Successful ability to stay at home - An interview study exploring multiple diagnosed older persons and their relatives’ experiences Running title: The meaning of ability to stay at home
Authors: Gustafsson Lena-Karin , Bondesson Anna , Pettersson Tina , Östlund Gunnel 
Published: April 2023

Abstract

Background: Society places increased demands on regions and municipalities to jointly carry out activities for multi-diagnosed older persons with extensive coordination needs. Interprofessional collaboration is reported as an important success factor for the overall health care of this group of patients. This project focuses on older persons with multiple diagnoses and their relatives’ own experiences of what is most important for safety and security in their homes.
The aim of the study was: to illuminate the meaning of success for the ability to stay at home as experienced by older persons with multiple diagnoses and their relatives.
Methods: The project had a descriptive explorative design with a phenomenological hermeneutic approach based on analysis of in-depth interviews.
Findings: Own resources were identified such as belief in the future, spiritual belief, social network, having loved ones and pets. Technical aids were seen as helpful resources, working as indoor and outdoor security safeguards. These resources included having good telephone contact with social and professional networks as well as other forms of personal equipment such as a personal alarm. The professional network was a resource, acting as support when the patient's own abilities were not sufficient. Finally, having personnel who had the time and interest to listen was seen as crucial to experience safety.
Conclusions: The main reason for being able to continue homecare was the person’s self-care system, their personal, social, and technical resources. Professional care development should anchor team work to the patient’s own system of self and informal care.
Fluoride concentration, antibacterial effect, and cytotoxicity in children’s toothpaste: in vitro study
Authors: Nantanapiboon Dusit , Thongthai Pasiree , Sivavong Pimduean , Osathanon Thanaphum , Tantilertanant Yanee , Sakoolnamarka Rangsima , Singthong Thawanrat , Uttasen Sirirat , Boonsuth Budsaraporn , Monmaturapoj Naruporn 
Published: April 2023

Abstract
Aim The aim of this study is to evaluate the effect on cell viability, the antibacterial activity against cariogenic bacteria, and the total bioavailability of commercially available children’s toothpastes in Thailand.Materials and methods Seven toothpaste slurries were prepared from seven commercial toothpastes for children. Total fluoride concentration was determined. Agar diffusion method was used to examine the antibacterial effect of toothpaste against Streptococcus mutans. The viability of L929 mouse fibroblasts exposed with different concentrations of each toothpaste slurry was tested by MTT assay.Results Fluoride concentration in fluoride containing toothpastes was ranged from 521.36 to 1377.83 ppm. Two toothpastes exhibited a similar level of fluoride concentration compared between the product labels. Other toothpastes showed the difference or slight difference in fluoride concentration between the companies’ information and our data. Zone of inhibition was ranged from 0-2.08 cm. Significant higher zone of inhibition was observed in toothpastes with sodium lauryl sulphate. The concentration of toothpaste that reduced cell viability to be less than 50% of the control was 0.8%, 3.1%, 6.3%, 6.3%, 25% and 100% for Fluocaril Deli Fruity, Oral-B Junior 6+, Kodomo kids, CUdent Stevia, Sun Star GUM and Kindee Organic, respectively.Conclusion The examined children’s toothpastes can inhibit the growth of S. mutans which did not correlate with fluoride concentration. The antibacterial effects could be the results of other ingredients, for example sodium lauryl sulphate.