bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telemedicine in the primary care of older adults: a systematic mixed studies review
Authors: Ilali Marwa , Le Berre Mélanie , Vedel Isabelle , Khanassov Vladimir 
Published: July 2023

Abstract
Background
Family physicians had to deliver care remotely during the COVID-19 pandemic. Their efforts highlighted the importance of developing a primary care telemedicine (TM) model. TM has the potential to provide a high-quality option for primary care delivery. However, it poses unique challenges for older adults. Our aim was therefore to explore the effects of TM and the determinants of its use in primary care for older adults.

Methods
In this systematic mixed studies review, MEDLINE, PsycINFO, EMBASE, CINHAL, AgeLine, DARE, Cochrane Library, and clinical trials research registers were searched for articles in English, French or Russian. Two reviewers performed study selection, data extraction and assessment of study quality. TM’s effects were reported through the tabulation of key variables. TM use determinants were interpreted using thematic analysis based on Chang’s framework. All data were integrated using a joint display matrix.

Results
From 3,328 references identified, 20 studies were included. They used either phone (n = 8), videoconference (n = 9) or both (n = 3). Among studies reporting positive outcomes in TM experience, ‘user habit or preferences’ was the most cited barrier and ‘location and travel time’ was the most cited facilitator. Only one study reported negative outcomes in TM experience and reported ‘comfort with patient communication’ and ‘user interface, intended use or usability’ as barriers, and ‘technology skills and knowledge’ and ‘location and travel time’ as facilitators.
Among studies reporting positive outcomes in service use and usability, no barrier or facilitator was cited more than once. Only one study reported a positive outcome in health-related and behavioural outcomes.

Conclusions
TM in older adults’ primary care generally led to positive experiences, high satisfaction and generated an interest towards alternative healthcare delivery model. Future research should explore its efficacy on clinical, health-related and healthcare services use.
The structural characterization and bioactivity assessment of nonspecific lipid transfer protein 1 (nsLTP1) from caraway (Carum carvi) seeds
Authors: Aldakhil Taibah , Alshammari Saud O. , Siraj Bushra , El-Aarag Bishoy , Zarina Shamshad , Salehi David , Ahmed Aftab 
Published: July 2023

Abstract
Background
Carum carvi (caraway) of the Apiaceae family has been used in many cultures as a cooking spice and part of the folk medicine. Previous reports primarily focus on the medicinal properties of caraway seed essential oil and the whole seeds extract. However, no effort has been made to study caraway proteins and their potential pharmacological properties, including nonspecific lipid transfer protein (nsLTP), necessitating further research. The current study aimed to characterize nonspecific lipid transfer protein 1 (nsLTP1) from caraway seed, determine its three-dimensional structure, and analyze protein–ligand complex interactions through docking studies. We also evaluated nsLTP1 in vitro cytotoxic effect and antioxidant capacity. Additionally, nsLTP1 thermal- and pH- stability were investigated.

Methods
Caraway nsLTP1 was purified using two-dimensional chromatography. The complete amino acid sequence of nsLTP1 was achieved by intact protein sequence for the first 20 residues and the overlapping digested peptides. The three-dimensional structure was predicted using MODELLER. Autodock Vina software was employed for docking fatty acids against caraway nsLTP1. Assessment of nsLTP1 cytotoxic activity was achieved by MTS assay, and the Trolox equivalent antioxidant capacity (TAC) was determined. Thermal and pH stability of the nsLTP1 was examined by circular dichroism (CD) spectroscopy.

Results
Caraway nsLTP1 is composed of 91 residues and weighs 9652 Da. The three-dimensional structure of caraway nsLTP1 sequence was constructed based on searching known structures in the PDB. We chose nsLTP of Solanum melongena (PDB ID: 5TVI) as the modeling template with the highest identity among all other homologous proteins. Docking linolenic acid with caraway protein showed a maximum binding score of -3.6 kcal/mol. A preliminary screening of caraway nsLTP1 suppressed the proliferation of human breast cancer cell lines MDA-MB-231 and MCF-7 in a dose‑dependent manner with an IC50 value of 52.93 and 44.76 μM, respectively. Also, nsLTP1 (41.4 μM) showed TAC up to 750.4 μM Trolox equivalent. Assessment of nsLTP1 demonstrated high thermal/pH stability.

Conclusion
To the best of our knowledge, this is the first study carried out on nsLTP1 from caraway seeds. We hereby report the sequence of nsLTP1 from caraway seeds and its possible interaction with respective fatty acids using in silico approach. Our data indicated that the protein had anticancer and antioxidant activities and was thermally stable.
GAS6-based CAR-T cells exhibit potent antitumor activity against pancreatic cancer
Authors: Fan Jiawei , Yu Ye , Yan Lanzhen , Yuan Yuncang , Sun Bin , Yang Dong , Liu Nan , Guo Jing , Zhang Jie , Zhao Xudong 
Published: July 2023

Abstract
Background
The receptor tyrosine kinases TAM family (TYRO3, AXL, and MERTK) are highly expressed in multiple forms of cancer cells and tumor-associated macrophages and promote the development of cancers including pancreatic tumor. Targeting TAM receptors could be a promising therapeutic option.

Methods
We designed a novel CAR based on the extracellular domain of growth arrest-specific protein 6 (GAS6), a natural ligand for all TAM members. The ability of CAR-T to kill pancreatic cancer cells is tested in vitro and in vivo, and the safety is evaluated in mice and nonhuman primate.

Results
GAS6-CAR-T cells efficiently kill TAM-positive pancreatic cancer cell lines, gemcitabine-resistant cancer cells, and cancer stem-like cells in vitro. GAS6-CAR-T cells also significantly suppressed the growth of PANC1 xenografts and patient-derived xenografts in mice. Furthermore, these CAR-T cells did not induce obvious side effects in nonhuman primate or mice although the CAR was demonstrated to recognize mouse TAM.

Conclusions
Our findings indicate that GAS6-CAR-T-cell therapy may be effective for pancreatic cancers with low toxicity.
A comprehensive integrated dataset on Brazilian health facilities: from 2005 to 2021
Authors: Salles Rebecca , de Paula Dornellas Ribeiro Victor , Gritz Raquel , Cardoso Carlos Leonardo Souza , Paixão Balthazar , Carraro Lucas , de Borba Cruz Sérgio Ricardo , Kreischer Vinicius , Bonifácio Carmen Lúcia Corrêa , de Freitas Saldanha Raphael , Souto Gabriel , Miloski Matheus , Zirondi Leandro , Alencar Gizelton Pereira , Alves Ariane Camilo Pinheiro , Neto Nelson Niero , da Costa Lima Jefferson , Pedroso Marcel , Alves Ronaldo Fernandes Santos , de Moraes Mello Boccolini Patricia , Boccolini Cristiano Siqueira 
Published: July 2023

Abstract
Objectives
The National Registry of Healthcare Facilities is a system with the registry of every healthcare facility in Brazil with information on the capacity building and healthcare workforce regarding its public or private nature. Despite being publicly available, it can only be accessed in separated disjoint tables, with different primary units of analysis. The objective is to offer an interoperable dataset containing monthly data from 2005 to 2021 with information on healthcare facilities, including their physical and human resources, services and teams, enriched with municipal information.

Data description
Database with historical data and geographic information for each health facility in Brazil. It is composed by 5 distinct tables, organized according to combinations of time, space, and types of resources, services and teams. This database opens up a range of possibilities for research topics, from case studies in a single health facility and period, analysis of a group of health facilities with characteristics of interest, to a broader study using the entire dataset and aggregated data by municipality. Furthermore, the fact that there is a row for each health facility/month/year facilitates the integration with other datasets from the Brazilian healthcare system. In addition to being a potential object of study in the health area, the dataset is also convenient in data science, especially for studies focused on time series.
Integration of microbiome and Koch’s postulates to reveal multiple bacterial pathogens of whitish muscle syndrome in mud crab, Scylla paramamosain
Authors: Hou Dongwei , Lian Taixin , Guo Guangyu , Gong Han , Wu Chengcheng , Han Peiyun , Weng Shaoping , He Jianguo 
Published: July 2023

Abstract
Background
For more than a century, the Koch’s postulates have been the golden rule for determining the causative agents in diseases. However, in cases of multiple pathogens-one disease, in which different pathogens can cause the same disease, the selection of microorganisms that regress infection is hard when Koch’s postulates are applied. Microbiome approaches can obtain relatively complete information about disease-related microorganisms and can guide the selection of target microorganisms for regression infection. In the present study, whitish muscle syndrome (WMS) of Scylla paramamosain, which has typical symptoms with whitish muscle and blackened hemolymph was used as an example to establish a new research strategy that integrates microbiome approaches and Koch’s postulates to determinate causative agents of multiple pathogens-one disease.

Results
Microbiome results revealed that Aeromonas, Acinetobacter, Shewanella, Chryseomicrobium, Exiguobacterium, Vibrio and Flavobacterium, and Kurtzmaniella in hemolymph were bacterial and fungal indicators for WMS. A total of 23 bacteria and 14 fungi were isolated from hemolymph and muscle tissues, and among the bacteria, Shewanella chilikensis, S. xiamenensis, Vibrio alginolyticus, S. putrefaciens, V. fluvialis, and V. parahaemolyticus were present in hemolymph and/or muscle tissues in each WMS crab, and the last three species were also present in three Healthy crabs. The target bacteria and fungi were further screened to regression infections based on two criteria: whether they belonged to the indicator genera for WMS, whether they were isolated from both hemolymph and muscle tissues in most WMS crabs. Only S. chilikensis, S. putrefaciens, S. xiamenensis, V. alginolyticus, V. fluvialis, and V. parahaemolyticus met both two criteria. The six bacteria that met both two criteria and six fungi and another bacterium that unmatched any of two criteria were used to perform regression infection experiments based on Koch’s postulates. S. chilikensis, S. putrefaciens, S. xiamenensis, V. alginolyticus, V. fluvialis, and V. parahaemolyticus met both two criteria, and the results indicate that they cause WMS in crabs independently.

Conclusions
This study fully demonstrated that our research strategy that integrates the microbiome and Koch’s postulates can maximize the ability to catch pathogens in one net for the situation of multiple pathogens-one disease.
Investigating the dark-side of the genome: a barrier to human disease variant discovery?
Authors: Ryan Niamh M. , Corvin Aiden 
Published: July 2023

AbstractThe human genome contains regions that cannot be adequately assembled or aligned using next generation short-read sequencing technologies. More than 2500 genes are known contain such ‘dark’ regions. In this study, we investigate the negative consequences of dark regions on gene discovery across a range of disease and study types, showing that dark regions are likely preventing researchers from identifying genetic variants relevant to human disease.
Percutaneous tracheostomy in patients with COVID-19 infection and acute respiratory failure
Authors: Singh Tushi , Sarwar Hasham , Hurtado Andres , Islam Ebtesam 
Published: July 2023

Background: Tracheostomy is often performed in patients who need prolonged intubation. COVID -19 brought unforeseen challenges, thus altering previously established norms. In this study, the outcomes of the patients undergoing tracheostomy for respiratory failure due to COVID -19 were studied.
Methods: This is a single center retrospective observational cohort study of patients who underwent percutaneous tracheostomies between March 1, 2020, and September 30, 2021, due to respiratory failure secondary to COVID-19. Inclusion criteria included performance of percutaneous tracheostomies on patients with confirmed diagnosis of COVID-19. Exclusion criteria included patients undergoing surgical tracheostomies, extubation prior to the performance of a tracheotomy, and death prior to the performance of the tracheotomy.
Results: The study included 49 patients after reviewing the records of 101 patients who underwent tracheostomies during the study period. The average age of the population was 59 ± 11years; 33 patients (67%) were men. The median Sequential Organ Failure Assessment (SOFA) score on admission was 2. The median duration of mechanical ventilation prior to tracheostomy was 18 days; the median positive end expiratory pressure was 10 cm H2O and the median fraction of inspired oxygen (FiO2) was 0.45. Two patients died during the procedure, one secondary to cardiac arrest and one secondary to bleeding. Eighteen patients (38%) died after the procedure during hospitalization; the median length of mechanical ventilation for all patients was 32.5 days. Eleven patients (22%) were eventually decannulated. Twenty patients (40%) were discharged to rehabilitation, and nine patients (18%) were discharged home. Eighteen patients (36%) were alive at the end of 90 days. Twelve patients (26%) were lost to follow up after discharge from the hospital. At the time of the tracheostomy, 16 patients (32%) had moderate ARDS as per the Berlin definition, and 12 (24%) had severe ARDS.
Conclusion: Tracheostomy is an important therapeutic intervention in critically ill patients requiring mechanical ventilation. The COVID-19 pandemic raised important concerns and uncertainties about the management of these patients and the safety of healthcare workers. In this study, 29 patients (59%) undergoing tracheostomies recovered enough to be discharged to rehabilitation or to their homes. The risks to patients and to healthcare workers seem reasonable, but the optimal timing is uncertain and is best tailored to each patient based on his/her clinical status and prognosis.

Keywords: COVID-19, tracheostomy, mechanical ventilation, acute respiratory failure
Healthcare and Behavior Changes for Adults With Down Syndrome 1-Year Into COVID-19
Authors: Rubenstein Eric , Kyprianou Nichole , Kumar Prisha Sujin , Kriova Anna-Mariya , Sokoloff Alexis , Hillerstrom Hampus , Hendrix James 
Published: July 2023

Abstract
Individuals with Down syndrome (DS) have been disproportionately harmed by the COVID-19 pandemic and may have been more likely to have sacrificed opportunity and activity to avoid potential exposures. Our objective was to describe the experience one to one and half years into the COVID-19 pandemic for adults with DS, as reported by their caregivers in an online survey conducted between April 2021 and September of 2021. In our sample of 438 adults with DS, caregivers reported that adults with DS lost activities, struggled with employment, had negative behavioral changes, lost skills, and developed more mental health conditions. For adults with DS, one in five caregivers reported less healthcare usage, one in four reported delayed routine care, and 86.5% reported lost activities. As the pandemic continues, targeted support for adults with DS is needed to prevent further skill loss and mental health conditions.
MASS MORTALITY OF CORALS AND REEF LIVING FEATURES AT CON DAO ARCHIPELAGO (VIETNAM) IN OCTOBER 2005
Authors: Ben Hoang Xuan , Tuan Vo Si , Hoang Phan Kim 
Published: July 2023

Con Dao archipelago located in the southeastern waters of Vietnam at 8037’ – 8048’ N and 106032’ - 106045’ E is characterised by diverse terrestrial and marine ecosystems. Coral reefs occupy the area around 1000 ha with more than 320 hard corals species recorded. A catastrophe causing mass mortally of corals and reef leaving features occurred in October 2005. Almost coral reefs in the northeastern waters of the archipelago was impacted, indicating mortality of corals ranking from 20% to nearly 100%. The mass mortality is more serious in reef flats in comparison with that in reef slopes. All hard corals of genera Acropora, Leptoria, Platygyra, Montipora and Porites died in reef flats meanwhile Galaxea is more resilient to the impact. Almost non-movable benthic invertebrates also died and density of reef fish decreased seriously on impacted reefs.The analysis of water samples and the data recorded by the Station of Meteorology and Hydrology at Con Dao showed the reason of the catastrophe. Water temperature in Con Dao increased with temperature more than 300C in lots of days during October, especially more than 310C on 11th and 12th October. In the same time, salinity also reduced with value less than 25%o in 7 days. Combination impact of these two factors were considered as the reason for mass mortality in the event. A hypothesis that fresh water from river mouths of the Mekong river system was dispersed to the archipelago during this period was recommended for further surveys.
Innovative low-cost engineered adsorbents based on eggshell waste for nickel removal from aqueous solutions
Authors: SEGNEANU Adina-Elena , TRUSCA Roxana , CEPAN Claudiu , MIHAILESCU Maria , MUNTEAN Cornelia , HEREA Daniel Dumitru , GROZESCU Ioan , SALIFOGLOU Athanasios 
Published: July 2023

Abstract

In contemporary sustainable economy, innovation is prerequisite to waste recycling into new efficient materials, designed to minimize pollution and conserve non-renewable natural resources. Water pollution is a global problem with health, quality of life, and food safety concerns. Thus, waste conversion into cheap, efficient adsorbent materials with high reusability is a challengein wastewater recycling. In that context, starting from eggshell waste in this study, two new low-cost engineered adsorbents were prepared for the retrieval of nickel from aqueous solutions. Scanning electron microscopy (SEM) results show that, in the first eggshell-zeolite (EZ) adsorbent, the zeolite nanoparticles were loaded in the eggshell pores. The preparation for the second (iron(III) oxide-hydroxide)-eggshell-zeolite (FEZ) adsorbent led to double functionalization of the eggshell base with the zeolite nanoparticles, upon simultaneous loading of the pores of the eggshell and zeolite surface with FeOOH particles. Structural modification of the eggshell led to a significant increase of the specific surface, as confirmed by BET analysis. These features enabled the proposed adsorbents (EZ and FEZ) to remove nickel from aqueous solutions with high performance and adsorption capacities 321.1 mg/g and 287.9 mg/g, respectively. The results indicate that nickel adsorption on EZ and FEZ is a multi-molecular layer, spontaneous, and endothermic process. Concomitantly, desorption results reflect high reusability of these two adsorbents, collectively suggesting the use of waste in the design of new, low-cost, and highly efficient engineered adsorbents for environmental bioremediation
Thermohydraulic Performance Analysis of Tube Heat Exchanger using Dual Side Serrated Insert Tape Having Triangular Wings
Authors: Sharma Sohan Lal , Singh Satyendra , Debbarma Ajoy 
Published: July 2023

Abstract

The thermal performance of heat exchangers can be improved by increasing the rate of heat transfer. Heat transmission and fluid flow characteristics have been improved in this experimental study by adopting a dual-side serrated insert tape with triangular wings. Different geometrical parameters such as wing pitch ratio (P/W), wing width ratio (w/W), wing depth ratio (d/W), serration width ratio (x/W), and perforation index (PI) are considered. The work is performed with various configurations of flow direction such as forward and backward flow with and without alternate axis using various ranges of Reynolds Number (i.e., 10000 ≤ Re ≤ 30000). According to the findings, the forward flow configuration of the insert performs better as compared to the backward flow. But the alternate axis with backward flow configurations and without perforation on the winglet improved thermal performance up to 1.05 times. In the alternate axis with forward flow configuration and with perforation on the winglet, the friction factor is at its lowest value. The Nusselt number and thermal performance factor enhanced up to 1.5 times, and 1.03 as compared to the plain tube.
A plant litter decay experiment unveiled anaerobic lignin decomposition in a rewetted peatland
Authors: Reuter Julia , Reuter Hendrik , Zak Dominik 
Published: July 2023

Abstract

The rewetting of long-term drained peatlands leads to the development of eutrophic shallow lakes, gradually inhabited by reed communities. These shallow lakes are characterized by significant nutrient and methane emissions. To gain a better understanding of the fate of organic compounds derived from decaying Phragmites australis litter in both the aquatic phase and underlying anaerobic soil layers, a decomposition experiment was conducted over a period of approximately 1.6 years. The experiment employed bulk and lignin-derived phenol analysis, as well as Fourier-transform infrared spectroscopy. As anticipated, the highest level of decomposition was observed in the surface water body of the shallow lake, while the non-rooted degraded peat exhibited the lowest decay. The bulk mass loss of plant litter decreased with depth from 55–27% across the four decomposition environments. Analysis using infrared spectroscopy indicated that the decrease in mass loss was primarily driven by the breakdown of carbohydrates, which constitute a significant portion of plant litter. Interestingly, the rooted degraded peat layer exhibited the highest degree of lignin decay. Furthermore, the study revealed a preferential loss of vanillin phenols and an accumulation of p-hydroxyl phenols. These findings suggest that the increased methane emissions in rewetted fens may be partially attributed to the demethoxylation of vanillin phenols and the subsequent formation of p-hydroxyl phenols. In conclusion, this study provides valuable insights into anaerobic lignin decomposition of plant litter and sheds light on potential mechanisms underlying methane emissions in rewetted peatlands.
Foscarnet eyedrops for the treatment of refractory herpetic keratitis
Authors: Awh Caroline C. , Knapp Austen N. , Goshe Jeffrey M. , See Craig W. , Lowder Careen Y. 
Published: July 2023

Abstract

Purpose
The purpose of this case series is to describe the clinical course of patients receiving foscarnet eyedrops for the treatment of refractory herpetic keratitis.
Observations:
Six patients diagnosed with herpetic keratitis were treated with foscarnet 24 mg/mL (2.4%) eyedrops with resulting improvement in keratitis.
Conclusion
Topical foscarnet may be a safe and effective treatment for herpetic keratitis in conjunction with, or as an alternative to, conventional antiviral therapy. This is an off-label use of foscarnet.