bluuebunny/crossref_metadata_2025_split huggingface dataset:
The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids
Authors: Suudi Muhammad 
Published: July 2023

matK is one of the universal loci which is commonly used as a barcode in the molecular identification of plants. Up to now, the problem of matK as a single locus is the use of the entire gene region as barcodes. The long amplicon size of matK raises several problems in DNA barcoding. The aim of this study is to uncover a small region of the matK gene as a potential mini-barcode region for alternative sites to get more efficient and effective results. In silico studies performed include the collection of matK sequence of medicinal orchid from GenBank, the amplicon size from matK primer sets that are commonly used in the Orchidaceae family, and PCR analysis. Data analysis was done by comparing the results of PCR amplification from several primer sets: matK 390F-1326R, 743F-R2, 712F-1154R and 712F-1216R. The results show that all primer pairs are able to amplify the DNA template isolated from Dendrobium purpureum. However, the shorter DNA band obtained from matK 743F-R2, 712F-1154R and 712F-1216R primers sets were visualized more thick than the longer amplicon band produced from matK primer 390F-1326R. This indicates that short matK sequence for DNA barcoding (mini-barcode) is more efficient in amplifying DNA templates compared to generally applied barcoding using full length sequences.
Ensino remoto emergencial e a antropologia filosófica na enfermagem
Authors: Silva Rodrigo Rodrigues , Malaquias Bruna Stephanie Sousa , Elias Henrique Ciabotti , Santos Álvaro da Silva 
Published: July 2023

Objetivo: relatar a experiência de ações inovadoras para o ensino de Antropologia Filosófica. Método: foi ministrada a disciplina de Antropologia Filosófica na graduação em enfermagem, em 10 encontros virtuais, por meio do Ensino Remoto Emergencial (ERE), com a aplicação de diversas metodologias ativas de ensino-aprendizagem (MAES). Resultados: a disciplina aplicou oito tipos de MAES e atividades lúdicas, além da produção de conteúdos específicos. A motivação para adesão e não abandono do ERE pelos alunos, a interatividade e a ressignificação de saúde sob o ponto de vista antropológico e filosófico foram desfechos importantes dessa intervenção educativa. Conclusão: a definição e aplicação criativa de MAES podem favorecer o ensino de Antropologia Filosófica e à ressignificação do ERE.
Exploring the Ways Arabic as a Foreign Language (AFL) Teachers Target Students’ Critical Thinking
Authors: Nfissi Abdelhamid , Elmzandi Jaouhara , Laayadi Soukaina 
Published: July 2023

<p><span lang="EN-US">Bloom’s taxonomy was created in 1956 by the educational psychologist Dr. Benjamin Bloom. It was created to promote higher-order thinking skills in the field of education, such as analyzing and evaluating concepts, procedures, and principles, rather than just retrieving facts. As a result, language teachers have access to varied ways to trigger and prompt students’ critical thinking by targeting their higher-order thinking skills. This qualitative research project explores how Moroccan AFL (Arabic as a Foreign Language) teachers target students’ critical thinking. This project focuses on the extent to which Moroccan AFL teachers trigger learners’ higher-order thinking skills, as well as the ways they target them. The research involved the observation of two lessons in a center of teaching Arabic as a foreign language. Three themes emerged from the data and were analysed</span><span>: <em>First</em>, adopting the role of critical thinking, namely the role of a guide and mediator, not as a teacher of knowledge. <em>Second</em>, using two ways of motivation: positive comments and repeating students' answers. <em>Third</em>, implement activities and questions that help students not to take things for granted.</span></p><p> </p><p><strong><span>Mengeksplorasi Cara Guru Pelajaran Arab sebagai Bahasa Asing (AFL) Menargetkan Pemikiran Kritis Siswa.</span></strong><span>Taksonomi Bloom yang diciptakan pada tahun 1956 Benjamin Bloom, psikolog pendidikan, bertujuan untuk mempromosikan keterampilan berpikir tingkat tinggi di bidang pendidikan, seperti menganalisis dan mengevaluasi konsep, prosedur, dan prinsip, bukan hanya mengambil fakta. Akibatnya, guru bahasa memiliki akses ke pelbagai cara untuk memicu dan mendorong pemikiran kritis siswa dengan menargetkan keterampilan berpikir tingkat tinggi mereka. Penelitian kualitatif ini mengeksplorasi bagaimana guru pelajaran Arab sebagai Bahasa Asing (AFL) Maroko menargetkan pemikiran kritis siswa, sehingga penelitian ini terfokus pada sejauh mana guru AFL Maroko memicu keterampilan berpikir tingkat tinggi peserta didik, serta cara mereka menargetkannya. </span><span lang="EN-US">Penelitian ini melibatkan </span><span>observasi terhadap </span><span lang="EN-US">dua pelajaran di pusat pengajaran bahasa Arab sebagai bahasa asing. </span><span>Penelitian </span><span lang="EN-US">ini menunjukkan cara seorang guru AFL Maroko menargetkan pemikiran kritis siswa</span><span>;</span><span lang="EN-US"> Pertama, mengadopsi peran berpikir kritis, yaitu peran sebagai pembimbing dan mediator, bukan sebagai guru sumber ilmu. Kedua, menggunakan dua cara motivasi, yaitu penggunaan komentar positif dan pengulangan jawaban peserta didik. Ketiga</span><span>,</span><span> <span lang="EN-US">menerapkan aktivitas dan pertanyaan yang membantu siswa untuk tidak menerima begitu saja.</span></span></p>
IADC Code Upgrade: Interpretation of Surface and Downhole Data to Support Drilling Forensics
Authors: Watson W. , Witt-Doerring Y. , Sugiura J. , Pastusek P. , Daechsel D. , Vallet L. , Amish M. , Oluyemi G. 
Published: July 2023

Summary
This paper establishes drilling surveillance interpretation and monitoring techniques for digital drilling data which can be used to support drilling forensics and improve drilling performance.
One significant advancement in the last 20 years has been the widespread availability and use of sensors to monitor all aspects of the drilling process. The majority of sensors will take surface and downhole data at several hundred samples per second, process the data, and store a record at one sample per second. The data from these sensors are collated and processed using some form of electronic data recording (EDR) system. The information is subsequently displayed in real time and stored for off-site transmittal. This paper extensively evaluates the impact on drilling performance due to how data from such sensors are collected and processed and the information is displayed.
A number of observations are investigated, analyzed, and explained identifying how data quality, consistency, frequency, sensor errors, and data artifacts can skew the displayed results. This can critically impact the drilling forensic analysis and subsequent interpretation. Failing to account for these data quality issues in real time may mask drilling dysfunction, causing accelerated damage to the drill bit and drilling assembly. This paper also aims to highlight techniques for displaying and interpreting drilling data to enhance drilling performance as well as diagnose dysfunction during reviews of historic wells. Understanding these limitations in advance and incorporating them in a team’s surveillance strategy can help with the diagnosis of drilling dysfunction and aid performance improvement.
These recommended practices have been developed to offer a foundation for drilling surveillance, interpretation, and monitoring as well as training for the industry. They have been created such that they can grow organically and may be used for developing subsequent industry publications.
The work described in this paper is part of a joint International Association of Drilling Contactors (IADC)/Society of Petroleum Engineers (SPE) industry effort to revise the IADC dull-grade process.
Antidepressant mechanism of Guipi Decoction revealed by network pharmacology and molecular docking
Authors: Wei Fangyuan , Song Jianxin , Pan Xiaoya , Zhang Xiangrong Dr. 
Published: July 2023

Abstract
Guipi Decoction is a famous Traditional Chinese Medicine formulae, which can be used to treat insomnia and depression. But its specific mechanism is still unclear. In this study, the active ingredients, targets and mechanisms of the Ziziphi Spinosae Semen-Poria cocos-Longan (ZPL) in Guipi Decocation was investigated by network pharmacology analysis and molecular docking. A total of 32 active ingredients, 344 intersection targets and 13 key targets were obtained. The result showed (S)-Coclaurine, coumestrol, n-trans-feruloyltyramine, ellagic acid, ellipticine and tartaric acid were the key compounds for depression. These compositions could reduce inflammatory response, inhibit pro-inflammatory cytokines, reduce neuronal apoptosis, and then modulate depression by the key targets of RAC-alpha serine/threonine-protein kinase (AKT1), tumor necrosis factor (TNF), interleukin (IL6), mitogen activated protein kinase 3 (MAPK3). Molecular docking results showed that the binding energy of n-trans-feruloyltyramine with PPARG was the lowest, -9.513 kcal/mol and the binding energy of (S)-Coclaurine and ESR1 was − 9.336. Upregulation of AKT1 gene inhibits apoptosis. Downregulation of TNF-α, MAPK and CTNNB1 genes reduces the expression of inflammatory factors and decreases the inflammatory response, which plays an important role in the treatment of depression. In conclusion, the active component of ZPL binds stably with AKT1, MAPK3, ESR1 and CTNNB1 and controlled the onset of depression by regulation of genes expression.
Effect of using drug management application on drug adherence and outcomes in older adults with polypharmacy: a randomized clinical trial study
Authors: Poorcheraghi Hossein , negarandeh reza , pashaeypoor shahzad , jorian Javad 
Published: July 2023

Abstract
Background
Adherence to complex drug regimens and polypharmacy are among the challenges of old age, which may negatively affect their motivation to continue drug therapy or lead to incorrect drug administration. The present study was conducted to evaluate the effect of using a drug management application on drug adherence and outcomes in polypharmacy older adults.
Methods
In this randomized clinical trial study conducted in 2022, 192 Iranian older adults with polypharmacy were selected according to the inclusion criteria and allocated to case and control groups using the block randomization method. The data collection tools included a demographic questionnaire, drug outcome questionnaire, and Morisky Medication Adherence Scale. The intervention was done using a drug management application for 8 weeks. The collected data were entered into the SPSS software version 22 and analyzed using descriptive (frequency, percentage, mean, standard deviation) and inferential (Chi-square, Fisher’s exact test, independent t-test) statistics.
Results
The case and control groups were homogeneous in terms of demographic variables and drug adherence level before the intervention. A significant difference was found in the drug adherence level after using the app (p < 0.001). Moreover, a significant difference was found in drug outcomes, including re-hospitalization due to disease aggravation, re-hospitalization due to medication error, falling, hypo or hypertension, and hypo or hyperglycemia, and medication use accuracy between the groups after the intervention (p < 0.05).
Conclusion
The results showed that using a drug management application that meets the specifications of older adults can improve drug adherence and reduce the adverse outcomes of drug use.
Brachial Plexopathy and Horner Syndrome as Presenting Symptoms for Non-Hodgkin’s Lymphoma
Authors: Labbad Ibrahim , Yasmeeneh Abeer , Mardini Majd , Abdulaziz Osama , Hamzeh Ghassan 
Published: July 2023

Abstract
Background:
the neurologic symptoms that appear due to paraneoplastic syndrome may give manifestations of undiagnosed tumor, but in this case brachial plexopathy accompanied with Horner syndrome were the first manifestations of non-Hodgkin lymphoma as paraneoplastic syndromes that preceded the diagnosis of the disease by about two years.
Case presentation:
in this case we report a 27 years old man who suffered from severe pain and weakness in the right upper limb, accompanied with right Horner syndrome.
Clinical discussion:
at the beginning of his complaint all the laboratory and radiographic investigations; CT scan, MRI for cervical spine and brachial plexus were negative except for the presence of marked thickening of the trunks, roots, and cords of the right brachial plexus that enhanced with gadolinium injection, after few months his symptoms developed include difficulty swallowing and hoarseness and lower extremities weakness, whole-body FDG-PET imaging was performed and showed right side cervical vertebral ganglia at the level of second and third vertebrae which was biopsied and showed: Diffuse large B-cell non-Hodgkin’s lymphoma. He began with treatment to which the tumor responded well.
Conclusion:
patients with brachial plexopathy, Horner syndrome, cervical spine myelopathy and peripheral neuropathy as manifestations for paraneoplastic syndrome of non-Hodgkin lymphoma are very rare and maybe this is the first case in worldwide.
Cdc6 activity analysis reveals possible unknown function in root meristem cells of Vicia faba
Authors: Musiałek Marcelina W. , Rybaczek Dorota 
Published: July 2023

Abstract
Cell division cycle 6 (Cdc6) protein regulates the licensing of DNA replication, maintains centrosome stability, participates in DNA damage response (DDR) pathways and activates cell cycle checkpoints. The mechanisms responsible for Cdc6 relocation in the cell cycle are evolutionary conserved. Here, we analyzed changes in Cdc6 activity following hydroxyurea-induced replication stress and caffeine-induced premature chromosome condensation (PCC). We used Vicia faba root meristem cells as a model to explore the dynamics of activity changes of Cdc6, Chk1 and Chk2 proteins and their phosphorylated forms (Cdc6Ser54ph, Chk1Ser345ph and Chk2Thr68ph) under the conditions of prolonged replication stress. We observed the areas of increased Cdc6 activity that were not reported for animal cells: (i) the presence of clusters associated with low-density chromatin (euchromatin), (ii) the frequent vicinity of Cdc6 clusters to the most dense regions of constitutive heterochromatin. Cdc6 remains within the nucleus throughout the interphase which may suggest its new, yet unknown function in plants. The results of changes in Cdc6 activity during prolonged replicative stress also indicate that Cdc6 plays an important role in maintaining the cell cycle checkpoint in S phase when stress conditions last longer.
Study on the Influence of Temporal and Spatial Resolution of Rainfall Data on Watershed Flood Simulation Performance
Authors: Pan Xinxin , Hou Jingming , Wang Tian , Li Xinyi , Jing Jing , Chen Guangzhao , Qiao Juan , Guo Qingyuan 
Published: July 2023

Abstract
To investigate the impact of temporal and spatial resolution of rainfall data on watershed flood simulation performance, the rainfall data from meteorological stations and the gridded rainfall data from meteorological forecasts for a rainfall event were adopted in this study. Interpolation methods were applied to generate rainfall processes with different spatial and temporal resolutions. A hydrodynamic model was employed to simulate the flow rates at various sections of the watershed under different rainfall scenarios. The results show that as the spatial and temporal resolutions decreased, the flood variation patterns at various sections remained consistent. Namely, the determination coefficient (R2) decreased, whereas the root means square error (RMSE) and mean absolute error (MAE) increased, and the errors in peak flow rates and the fluctuation amplitudes of the flow rates at the sections increased as well. Moreover, a decrease in temporal resolution led to a delay in the peak flow timing. Significant differences were observed between the simulation results generated from the two different rainfall datasets. The R2 values for the simulated flow rates at each section were all above 0.75 for the observed rainfall data, while 40% of the results based on meteorological forecast data were below 0.5. Overall, the simulation results using observed rainfall data outperformed those using meteorological forecast data. Through the comparative analysis of simulation results including the rainfall characteristic parameters such as the watershed-averaged precipitation (AVP) and the coefficient of variation (CV), it was found that AVP had a strong correlation with the peak flow and its increase or decrease directly affected the peak flow. On the contrary, CV showed a negative correlation with the peak flow.
A Situational Analysis of Ear and Hearing Care in South Korea Using WHO Ear and Hearing Care Situation Analysis Tool
Authors: Yoon Chul Young , Lee Juhyeong , Lee Junhun , Kong Tae Hoon , Seo Young Joon 
Published: July 2023

Abstract
Objectives: The World Health Organization emphasizes lifelong management of hearing diseases and suggests a state-led management program. In addition, the 'Ear and Hearing Care Situation Analysis Tool ' was produced and distributed so that the state-led management program could be implemented well. Therefore, the purpose of this study is to fill 'Ear and Hearing Care Situation Analysis Tool ', understand the current situation of ear and hearing management in Korea, establish a basis for establishing and improving policies related to hearing diseases, and produce data to report to World Health Organization.
Methods: The 'Ear and Hearing Care Situation Analysis Tool 'consists of a total of two sections, 41 questions in Section 1 and 203 questions in Section 2. Section 1 consists of questions that can evaluate health policies and support services. Section 2 consists of questions to evaluate the services and human resources of the ear and hearing management sector.
Results: Policies such as hearing aid support are in place, and outreach services such as free hearing tests are also being actively conducted. In all medical institutions, ear and hearing management treatment could be received, and medication prescriptions could be received without distinction. Workers such as audiologists, language therapists, special education teachers, and sign language interpreters, were specialized because they had well-established guidelines for training.
Conclusion: Overall, the domestic ear and hearing management sector has confirmed that policies and services are well prepared close to advanced countries compared to the current status of countries. It can be reported to World Health Organization to help improve domestic ear and hearing management policies and expand medical services.
Fcγ-receptor-independent controlled activation of CD40 canonical signaling by novel therapeutic antibodies for cancer therapy
Authors: Reitinger Carmen , Beckmann Karsten , Carle Anna , Blümle Eva , Jurkschat Nicole , Paulmann Claudia , Prassl Sandra , Kazandijan Linda V. , Nimmerjahn Falk , Fischer Stephan 
Published: July 2023

Abstract
Activation of CD40-mediated signaling in antigen-presenting cells is a promising therapeutic strategy to promote immune responses against tumors. Most agonistic anti-CD40 antibodies currently in development require Fcγ-receptor (FcγR)-mediated crosslinking of CD40 molecules for meaningful activation of CD40 signaling but have limitations due to dose-limiting toxicities. Here we describe the identification of CD40 antibodies which strongly stimulate antigen-presenting cells in an entirely FcγR independent manner. These Fc-silenced anti-CD40 antibodies induce and efficient upregulation of costimulatory receptors and cytokine release by dendritic cells. Binding to the CD40L interaction region on CD40 appears to be a prerequisite to achieving such strong activities. Finally, the most active identified anti-CD40 antibody shows activity in humanized mice. More importantly, there are no signs of obvious toxicities whereas the clinical-stage anti-CD40 antibody CP-870,893 induced severe signs of toxicity even at a lower dose. These studies thus demonstrate potent activation of antigen-presenting cells with anti-CD40 antibodies lacking FcγR-binding activity and open the possibility for an efficacious and safe combination therapy for cancer patients.
Convergence Results on the General Inertial-Mann-Halpern and General Inertial Mann Algorithms
Authors: Gebregiorgis Solomon , Kumam Poom 
Published: July 2023

Abstract
In this paper, we prove strong convergence theorem of the general inertial Mann-Halpern algorithm for nonexpansive mappings in the setting of Hilbert spaces.we also prove weak convergence theorem of the general inertial Mann algorithmfor k-strict pseudo-contractive mappings in the setting of Hilbert spaces. These convergence results extend and generalize some existing results in the literature.Finally, we provide examples to verify our main results.
Genome-wide investigation to assess copy number variants in the Italian local chicken population
Authors: Cendron Filippo , Cassandro Martino , Penasa Mauro 
Published: July 2023

Abstract
Background
Copy number variation (CNV) holds significant functional and evolutionary importance. Numerous ongoing CNV studies aim to elucidate the etiology of human diseases and gain insights into the population structure of livestock. High-density chips have enabled the detection of CNV with increased resolution, leading to the identification of even small CNV. This study aimed to identify CNV in local chicken breeds and investigate their distribution within the genome.
Results
The distribution of CNV was more prominent in the first six chromosomes and primarily associated with loss types. Most CNV among breeds were of types 0 and 1, and the minimum length of CNV was significantly larger than that reported in previous studies. Interestingly, chromosome 16 had a high proportion of its length covered by copy number variation regions (CNVR), with the major histocompatibility complex being the likely cause. Among the identified genes within CNVR, only those present in at least three animals across breeds (n = 95) were discussed to reduce the focus on redundant CNV. Some of these genes have been associated to functional traits in chickens. Notably, several CNVR on different chromosomes harbor genes related to muscle development, tissue-specific biological processes, heat stress resistance, and immune response. Quantitative trait loci (QTL) were also analyzed to investigate potential overlapping with the identified CNVR: 54 out of the 95 gene-containing regions overlapped with 428 QTLs associated to body weight and size, carcass characteristics, egg production, egg components, fat deposition, and feed intake.
Conclusions
Genomic phenomena that can cause changes in the distribution of CNV within the genome over time and the comparison of the differences among the CNVR of the local chicken breeds reported in the present study could help in their conservation.