bluuebunny/crossref_metadata_2025_split huggingface dataset:
Opsoclonus-Myoclonus-Ataxia Syndrome with Parainfective Etiology: A Case Report and Evaluation of Treatment Response Using the Mitchell Pike OMS-Rating Scale
Authors: Khan Muhammad Usman , Nawaz Ahmad , Numan Ahsan , Iftikhar Sadaf , Arshad Waqas , Farooq Sana , Shahid Seemab 
Published: August 2023

Abstract
Opsoclonus-myoclonus-ataxia syndrome (OMS), also known as Kinsbourne syndrome or dancing eyes-dancing feet syndrome, is a variable constellation of neurological symptoms. The majority of reported adult cases have been paraneoplastic, often associated with antineuronal nuclear antibody type 2 (ANNA-2; anti-Ri). However, its association with parainfective triggers remains underexplored in scientific literature. We present a unique and rare case of OMS diagnosed as secondary to acute viral cerebellitis. The patient exhibited opsoclonus, myoclonus, and ataxia, with cerebrospinal fluid analysis revealing lymphocytic pleocytosis and mildly elevated protein levels, indicative of a viral etiology. Investigations ruled out paraneoplastic OMS. The patient was managed with acyclovir, plasmapheresis, and steroids, resulting in a significant improvement in the Mitchell Pike scale score. The combined treatment approach led to progressive neurological improvement. This case report emphasizes the potential role of antiviral therapy, plasmapheresis, and steroids in managing parainfective OMS. It highlights the importance of considering viral etiologies in OMS cases and underscores the benefits of this therapeutic strategy. Furthermore, this case report also highlights the utility of the Mitchell Pike OMS-Rating Scale for the evaluation of treatment response. Clinicians should be diligent in investigating parainfective triggers and individualizing treatment approaches to optimize outcomes in OMS patients.
Elevated serum IP-10/CXCL10 levels are associated with sarcopenia development, a prognostic factor, in patients with primary hepatocellular carcinoma
Authors: Takada Hitomi , Yamashita Koji , Osawa Leona , Komiyama Yasuyuki , Muraoka Masaru , Suzuki Yuichiro , Sato Mitsuaki , Kobayashi Shoji , Yoshida Takashi , Takano Shinichi , Maekawa Shinya , Enomoto Nobuyuki 
Published: August 2023

Abstract
Sarcopenia is a prognostic factor in patients with hepatocellular carcinoma (HCC). However, the mechanism underlying sarcopenia development in patients with HCC remains unclear. The chemokine interferon-gamma-induced protein 10/C-X-C motif chemokine ligand 10 (IP-10/CXCL10) has emerged as one of the mechanisms in previous studies. Therefore, we aimed to elucidate the significance of sarcopenia, and investigate the association between serum IP-10/CXCL10 levels and sarcopenia development. This retrospective study included 738 patients with primary HCC, and among these patients, serum IP-10/CXCL10 levels ​​were measured both at baseline and after 1–3 years in a subset of 135 patients. Among patients with primary HCC, those with sarcopenia at baseline had a poorer prognosis than those without, and patients with sarcopenia at 1, 3, and 5 years after the first occurrence of HCC had a poorer prognosis. Furthermore, serum IP-10/CXCL10 ratios were found to be higher in patients with sarcopenia at baseline and those who developed sarcopenia during the observation period than in those without sarcopenia (p = 0.0016). This study revealed that the significance of sarcopenia as a prognostic factor in patients with HCC, and the changes in serum IP-10/CXCL10 levels appear to be associated with the development of sarcopenia following the first occurrence of HCC.
OpenMS 3 expands the frontiers of open-source computational mass spectrometry
Authors: Sachsenberg Timo , Pfeuffer Julianus , Bielow Chris , Wein Samuel , Jeong Kyowon , Netz Eugen , Walter Axel , Alka Oliver , Nilse Lars , Colaianni Pasquale , McCloskey Douglas , Kim Jihyung , Rosenberger George , Bichmann Leon , Walzer Mathias , Veit Johannes , Boudaud Bertrand , Bernt Matthias , Patikas Nikolaos , Pilz Matteo , Startek Michał Piotr , Kutuzova Svetlana , Heumos Lukas , Charkow Joshua , Sing Justin , Feroz Ayesha , Siraj Arslan , Weisser Hendrik , Dijkstra Tjeerd , Perez-Riverol Yasset , Röst Hannes , Kohlbacher Oliver 
Published: August 2023

Abstract
Mass spectrometry has become an indispensable tool in the life sciences. The new major version 3 of the computational framework OpenMS provides significant advancements regarding open, scalable, and reproducible high-throughput workflows for proteomics, metabolomics, and oligonucleotide mass spectrometry. OpenMS makes analyses from emerging fields available to experimentalists, enhances computational workflows, and provides a reworked Python interface to facilitate access for bioinformaticians and data scientists.
Removal of pharmaceuticals and personal care products from wastewater via anodic oxidation and electro-Fenton processes: current status and needs regarding their application
Authors: Lozano Iván , Cervantes-Aviles Pabel , Keller Arturo , Aguilar Claudia López 
Published: August 2023

Abstract

This review provides a current opinion on the most recent works that have been published toward the application of electrochemical advance oxidation processes (EAOPs) for the degradation of pharmaceutical and personal care products (PPCPs) in water streams. Advances in the application of anodic oxidation (AO)- and electro-Fenton (EF)-based processes are reported, including operational conditions, electrode performance, and removal. Although AO- and EF-based processes can easily reach 100% removal of PPCPs, mineralization is desirable to avoid the generation of potential toxic byproducts. The following section exploring some techno-economic aspects of the application of EAOPs is based on electrode selection, operational costs as well as their use as cotreatments, and their synergistic effects. Finally, this short review ends with perspectives about the emerging topics that are faced by these technologies applied for the degradation of PPCPs in research and practice.
Mitochondrial unfolded protein response (mtUPR) and diseases
Authors: Sun Lin , Yang Ming , Luo Shilu , Chen Wei , He Liyu , Liu Di , Wang Xi , Xiao Li 
Published: August 2023

Abstract:
Mitochondria are the energy factories of cells, and their functions are closely related to cell homeostasis. The mitochondrial unfolded protein response (mtUPR) is a newly discovered mechanism for regulating mitochondrial homeostasis. When unfolded/misfolded proteins accumulate in mitochondria, the mitochondria release signals that regulate the transcription of certain proteins in the nucleus, thereby inducing the correct folding or degradation of proteins in mitochondria. Many studies have also shown that an abnormality of mtUPR is closely related to the occurrence and development of diseases. Here, we summarized the pathways regulating mtUPR signaling and reviewed the research progress on mtUPR in diseases. Finally, we summarized the currently identified agonists and inhibitors of the mtUPR and discussed the potential of the mtUPR as a therapeutic target for diseases.
Evaluation of Treatments with Radiotherapy Alone and Radiotherapy Plus Chemo-immunotherapy in Patients with Primary Liver Cancer based on Blood Biomarkers
Authors: Huang Shigao , Yin Yutian , Li Jianping , Shi Mei , Bian Huijie , Zhao Lina 
Published: August 2023

Purpose:
It is critical to assess primary liver cancer patients likely to benefit from radiotherapy
(RT) or RT plus chemo-immunotherapy. Many potential peripheral biomarkers from blood
samples have been proposed for clinical application. Therefore, the aim of this study was to evaluate
treatments with radiotherapy alone and radiotherapy plus chemo-immunotherapy in patients
with unresectable primary liver cancer based on blood biomarkers.

Methods:
From January, 2017, to February, 2022, 63 unresectable primary liver cancer patients receiving
radiotherapy alone (RT, n = 21) or radiotherapy plus chemo-immunotherapy (RT plus
C/IT, n = 42) were included in this study. We compared the clinical outcomes and adverse effects
of these two groups. Also, distant metastasis-free survival (DMFS), overall survival (OS), and progress-
free survival (PFS) were retrospectively analyzed. Finally, univariable and multivariable Cox
analyses were used to explore the prognostic role of blood biochemical biomarkers.

Results:
In this study, 1, 2, and 3 years of OS after RT treatment were 63.9%, 27.0%, and 13.5%,
and after RT plus C/IT were 68.2%, 37.0%, and 24.7%, respectively (p = 0.617). Compared with
baseline, white blood cells (WBC) and lymphocytes were significantly decreased after RT (p =
0.002 and p = 0.001, respectively) or RT plus C/IT therapy (p = 0.135 and p<0.001, respectively).
In multivariable Cox regression analyses, higher lymphocyte counts before RT (pre-Lymphocyte)
were associated with better OS and PFS (HR=0.439, p = 0.023; HR=0.539, p = 0.053; respectively),
and higher lymphocyte counts before RT (pre- Platelets) were a poor prognostic factor associated
with DMFS (HR=1.013, p = 0.040). Importantly, OS and PFS were significantly better for patients
(pre-Lymphocyte ≥1.10 x 109/L) (p = 0.006; p = 0.066, respectively). The DMFS was significantly
better for patients (pre-platelets < 233.5 ×109/L) (p<0.001).

Conclusion:
Our evaluation of blood biomarkers before and after radiotherapy or plus chem-immunotherapy
for primary liver cancer revealed a potential marker for clinics to decide on precise
treatment strategies.
Eight Weeks of Vitamin C Supplementation Restores the Lost Correlation
between Serum Leptin and C-reactive Protein (CRP) in Patients with Type 2
Diabetes; A Randomized, Double-blind, Parallel-group, Placebo-controlled
Clinical Trial
Authors: Mohammadi Ali , Rabizadeh Soghra , Mirmoosavi Saeed , Alemi Hamid , Mirmiranpoor Hossein , Bagheri Sayna , Moradi Kamyar , Esteghamati Alireza , Nakhjavani Manouchehr 
Published: August 2023

Objective:
Inflammation is a well-described factor in the pathophysiology of type 2 diabetes mellitus
(DM), which has been a suspect in the alteration of correlations between CRP and leptin in patients with type 2
DM.

Aim:
This study aimed to show the effect of vitamin C as an antioxidant on the correlation of the serum levels
of C-reactive protein (CRP) and leptin in patients with type 2 DM.

Methods:
We recruited 70 patients with longstanding T2DM and randomly assigned them into two groups; one
received 500 mg/day of vitamin C, and the other received a placebo for eight weeks. Both groups were matched
regarding baseline characteristics such as age, gender, weight, and diabetic medications.

Results:
Out of 70 individuals, 57 participants were left in the study. After eight weeks of follow-up, leptin level
was significantly increased in the Vitamin C group (MD = 3.48 change = 24%, p-value = 0.001) but did not
change in the placebo group. Other markers such as Fasting plasma glucose, HbA1c, Creatinine, uric acid,
Urea, cholesterol, HDL, LDL, TG, AST, ALT, insulin, and CRP did not significantly change in both groups (p
value > 0.05). The significant changes in the leptin level among the vitamin C group also remained after controlling
for age, BMI, Blood pressure (BP), Triglyceride (TG), and cholesterol. Also, the correlation between
serum CRP and leptin became significant in the vitamin C group after eight weeks of follow-up but not in the
placebo group. (rs = 0.730, p < 0.001 vs. rs = 0.286, p-value = 0.266 in placebo group).

Conclusion:
This study shows vitamin C can restore CRP-leptin correlation in patients with type 2 diabetes and
increase serum leptin levels. More studies are needed to clarify the mechanism of this restoration.

Clinical Trial Registration Number:
IRCT20160811029306N1.
Protein Engineering in Cyanobacterial Biotechnology: Tools and Recent
Updates
Authors: Tyagi Swati , Kar Srabani , Srivastava Amit , Shukla Pratyoosh 
Published: August 2023

Abstract:
Cyanobacteria have emerged as a microbial cell factory to produce a variety of bioproducts,
including peptides and proteins. Cyanobacteria stand out among other organisms due to their
photoautotrophic metabolism and ability to produce a wide range of metabolites. As photoautotrophic
hosts can produce industrial compounds and proteins by using minimal resources such as
sunlight, atmospheric carbon dioxide, and fewer nutrients, cyanobacteria are cost-effective industrial
hosts. Therefore, the use of protein engineering tools for rational protein design, and the desired
modification of enzyme activity has become a desirable undertaking in cyanobacterial biology.
Protein engineering can improve their biological functions as well as the stability of their intracellular
proteins. This review aims to highlight the success of protein engineering in the direction
of cyanobacterial biotechnology and outlines the emerging technologies, current challenges, and
prospects of protein engineering in cyanobacterial biotechnology.
Current Insight on the Role of Glucokinase and Glucokinase Regulatory
Protein in Diabetes
Authors: Paliwal Ajita , Paliwal Vartika , Jain Smita , Paliwal Sarvesh , Sharma Swapnil 
Published: August 2023

Abstract:
The glucokinase regulator (GCKR) gene encodes an inhibitor of the glucokinase enzyme
(GCK), found only in hepatocytes and responsible for glucose metabolism. A common GCKR coding
variation has been linked to various metabolic traits in genome-wide association studies. Rare GCKR
polymorphisms influence GKRP activity, expression, and localization. Despite not being the cause,
these variations are linked to hypertriglyceridemia. Because of their crystal structures, we now better
understand the molecular interactions between GKRP and the GCK. Finally, small molecules that
specifically bind to GKRP and decrease blood sugar levels in diabetic models have been identified.
GCKR allelic spectrum changes affect lipid and glucose homeostasis. GKRP dysfunction has been
linked to a variety of molecular causes, according to functional analysis. Numerous studies have
shown that GKRP dysfunction is not the only cause of hypertriglyceridemia, implying that type 2 diabetes
could be treated by activating liver-specific GCK via small molecule GKRP inhibition. The review
emphasizes current discoveries concerning the characteristic roles of glucokinase and GKRP in
hepatic glucose metabolism and diabetes. This information has influenced the growth of directed molecular
therapies for diabetes, which has improved our understanding of lipid and glucose physiology.
Connection-Based Zagreb Indices of Polycyclic Aromatic Hydrocarbons Structures
Authors: Usman Muhammad , Javaid Muhammad 
Published: August 2023

Background::
Topological indices (TIs) are mathematical formulas that are applied in mathematical chemistry to predict the physical and chemical properties of various chemical structures. In this study, three different types of polycyclic aromatic hydrocarbon structures (PAHs) (i.e., Hexa-peri-hexabenzocoronene, Dodeca-benzo-circumcoronene, and Hexa-cata- hexabenzocoronene) are studied with the help of the different connection number-based Zagreb indices.

Materials and Methods:
ϑ = (V(ϑ),E(ϑ)) is used as a graph, where V(ϑ) is a collection of vertices and E(ϑ) is a collection of edges. For a vertex y, ∈V(ϑ), the degree d_ϑ (y), is the number of those vertices that are at a distance of 1 from y and the connection number ρ_ϑ (y) is the number of such vertices that are at a distance of 2 from vertex y.

Results::
Theoretical applications of topological indices were described in detail

Conclusion::
Finally, we obtained the first and second Zagreb connections as well as the modified first, second, third, and fourth Zagreb connection indices, which were calculated for three different types (Hexa-peri-hexabenzocorone, Dodeca-benzo-circumcoronene, and Hexa-cata-hexabenzocoronene) of polycyclic aromatic hydrocarbon structures.
Potent Small Molecules Inhibitors Discovery through Ligand-based Modelling for Effective Treatment of Parkinson’s Disease
Authors: Yahaya Sani Najib , Oloruntoyin Yusuf Ayipo , Alananzeh Waleed Abdullah Ahmad , Ajmal Amar , Shams Sulaiman , Wadood Abdul , Bn Mordi Mohd Nizam 
Published: August 2023

Background:
Parkinson’s disease (PD) is a chronic neurodegenerative disease affecting mostly aged people. The disease's symptoms develop gradually over time and include tremors, bradykinesia, rigidity, and postural instability. Current treatment options for PD are only symptom-targeted. Prolyl oligopeptidase (POP) is a serine protease enzymes implicated in PD pathogenesis via an increase in the aggregation of α-synuclein protein in the brain.

Aim:
This study aims to identify potent anti-PD ligands with inhibitory potential against POP

Methods:
Ligand-based pharmacophore modeling, Glide extra precision (XP) docking, and post-simulation analysis methods were used.

Results:
The adopted ligand-based (LB) modeling generated pharmacophoric features, including 1 hydrophobic group, 1 positive ionizable group, 2 aromatic rings, and 2 hydrogen bond acceptors. A total of 23 hits with a Gunner-Henry score of 0.7 and an enrichment factor of 30.24 were obtained as validation protocols, making it an ideal model. The LB model retrieved 177 hit compounds from the 69,543 natural screening ligands available in the Interbioscreen database. Interestingly, ligands 1, 2, 3, 4, and 5 orderly demonstrated higher binding affinities with Glide XP docking of -9.0, -8.8, -8.7, -8.7, -8.7 kcal/mol compared to reference drugs, GSK552 and ZPP with -8.2, and -6.8 kcal/mol respectively. Similarly, their MM/GBSA values were recorded as -54.4, -51.3, -58.4, -49.3, - 33.5, & -32.5 kJ/mol respectively. Further, MD analysis indicated that ligands had higher favorable binding and stability to the receptor.

Conclusion:
Overall, the study paves the way for developing potential anti-PD therapeutics. The ligands are recommended as adjuvant/single candidate as anti-PD candidates upon further experiment.
Phytosomal Curcumin and Shilajit Decrease Adhesion Bands Post-achilles Tendon Surgery in Animal Model
Authors: Asgharzadeh Fereshteh , Nazari Seyedeh Elnaz , Naeimi Hamideh , Rahmani Farzad , Avan Amir , Khazaei Majid , Hassanian Seyed Mahdi 
Published: August 2023

Aim:
This study aimed to investigate the protective effects of phytosomal curcumin and/or
shilajit in reducing post-surgical tendon adhesion band formation in a rat model.

Background:
Tendon adhesion is one of the severe complications after tendon surgery which causes limited
tendon movement and functional disability.

Objective:
According to the central role of inflammatory reactions in fibrosis and the formation of tendon
adhesions, we investigated the therapeutic effects of phytosomal curcumin and shilajit either alone or in
combination on reducing post-surgical tendon adhesion band formation in a rat model.

Methods:
We randomly divided 36 Wistar male rats into six equal groups. (A) Control group with no
surgical incision and no intervention. (B) Sham group with surgical incision, but no adhesion, (C) Positive
control group with total surgical transection and adhesion receiving normal saline daily, (D-F) Treatment
groups which are the same as group C except that rats were treated with the following drugs. Phytosomal
curcumin alone (250 μL/day; orally), shilajit alone (500 mg/kg/day; orally) and a combination of phytosomal
curcumin + shilajit. In groups with surgical intervention, the rats' Achilles tendons were cut and
repaired with a modified Kessler technique. At 3 weeks, all rats were euthanized. Histological and pathological
scoring systems were used to evaluate the protective effects of phytosomal curcumin and/or shilajit
in reducing adhesion bands at the site of tendon injuries.

Results:
Our results revealed that the administration of phytosomal curcumin and/or shilajit remarkably
reduced length, density, grading, severity, and thickness of post-surgical adhesion bands. Compared to the
untreated control group, the histological changes and inflammatory reactions were significantly attenuated
in treated rats. Furthermore, treatment with phytosomal curcumin and/or shilajit inhibited fibrotic responses
by alleviating collagen deposition, fibrosis quantity, fibrosis grading, and total fibrosis scores, as
visualized by Masson’s trichrome staining.

Conclusion:
Our findings indicated the anti-inflammatory and anti-fibrotic properties of phytosomal curcumin
and/or shilajit supporting their therapeutic potential in preventing post-operative tendon adhesion bands.
The Synthesis and Pharmacological Activity of Ursolic Acid Derivatives Modified at C-28 and C-3 Sites
Authors: Deng Jiawen , Zhang Si , Tan Yan , Zhou Jing , Liu Meiling , Yang Zehua , Yao Xu , Mi Pengbing , Zheng Xing 
Published: August 2023

:
Ursolic acid (UA) is a widely distributed triterpenoid in nature. Due to its easy availability and
multiple pharmacological activities, ursolic acid has attracted much attention in the field of medicine and
pharmacology. To overcome the disadvantages of bioavailability and poor water solubility during the
ursolic acid application, we focused on the synthetic and medicinal properties of UA derivatives modified
at C-3 and C-28 sites. This review presents the synthesis of UA derivatives with modification at C-3 and
C-28 sites and their pharmacological activity, which may provide some important information for further
research and development of UA-based drugs.
Invasive Fungal Infections in the Paediatric Intensive Care Unit: A Hong
Kong Study
Authors: Li Mario Wai Tung , Hon Kam Lun , Yan Leung Karen Ka , Hui Wun Fung , Lung David Christopher , Ha Shau Yin 
Published: August 2023

Introduction:
Invasive fungal infections (IFI) cause significant mortality and morbidity in the Paediatric Intensive Care Unit (PICU). Early recognition and prompt treatment of invasive fungal infections are important. This article reviewed the mortality and morbidity of IFIs in the PICU of Hong Kong Children’s Hospital.

Methods:
A retrospective review of all PICU admissions from April 2019 to May 2021 was per-formed. The following data were retrieved: age, gender, diagnosis, comorbidity, clinical manifestation, type of fungus, duration of stay at PICU, absolute neutrophil count, use of immunosuppressive therapy, presence of central venous catheter and use of total parental nutrition. The primary out-comes were the incidence and mortality of IFIs among PICU patients. The secondary outcomes were risk factors for developing IFI in PICU and clinical course of IFIs. Numerical variables were compared between groups by Mann-Whitney U test and categorical variables by Fisher’s exact test.

Results:
There were 692 PICU admissions over the study period from April 2019 to May 2021. The crude mortality was 3% (n=24 death cases) in the PICU. Fourteen patients (2%) fulfilling the criteria for IFIs were identified using hospital electronic record system and according to PICU documentation. Eight of these 14 patients (57%) had hematological malignancy, 2 (17%) had solid tumours and 4 had non-oncological conditions. Eight (57%) patients were neutropenic with absolute neutrophil count less than 1x 109 at diagnosis of IFI. Ten (71%) had received immunosuppressive therapy including steroid, cyclosporin A, Mycophenolate mofetil (MMF), Sirolimus or tacrolimus. 12 (86%) had had central venous catheter. Eight (57%) were on parenteral nutrition. IFIs due to Rhizopus or Aspergillus infection (5/14), or in post-haematopoietic stem cell transplant patients (5/14) were as-sociated with non-survival (p = 0.031).

Conclusion:
All patients with IFIs managed in the PICU had haemato-oncology diseases or were recipients of stem cell transplantation. IFIs with Rhizopus or Aspergillus as a group were associated with high mortality in the PICU. Awareness of this pathology with prompt diagnosis and treatment may improve the outcome of these infections and reduce the mortality.