bluuebunny/crossref_metadata_2025_split huggingface dataset:
CTGF promotes the repair and regeneration of alveoli after acute lung injury by promoting the proliferation of subpopulation of AEC2s
Authors: Sun Jianhui , Zhang Huacai , Liu Di , Liu Wenyi , Du Juan , Wen Dalin , Li Luoxi , Zhang Anqiang , Jiang Jianxin , Zeng Ling 
Published: September 2023

Abstract
Background
Functional alveolar regeneration is essential for the restoration of normal lung homeostasis after acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Lung is a relatively quiescent organ and a variety of stem cells are recruited to participate in lung repair and regeneration after lung tissue injury. However, there is still no effective method for promoting the proliferation of endogenous lung stem cells to promote repair and regeneration.

Methods
Using protein mass spectrometry analysis, we analyzed the microenvironment after acute lung injury. RNA sequencing and image cytometry were used in the alveolar epithelial type 2 cells (AEC2s) subgroup identification. Then we used Sftpc+AEC2 lineage tracking mice and purified AEC2s to further elucidate the molecular mechanism by which CTGF regulates AEC2s proliferation both in vitro and in vivo. Bronchoalveolar lavage fluid (BALF) from thirty ARDS patients who underwent bronchoalveolar lavage was collected for the analysis of the correlation between the expressing of Krt5 in BALF and patients’ prognosis.

Results
Here, we elucidate that AEC2s are the main facultative stem cells of the distal lung after ALI and ARDS. The increase of connective tissue growth factor (CTGF) in the microenvironment after ALI promoted the proliferation of AEC2s subpopulations. Proliferated AEC2s rapidly expanded and differentiated into alveolar epithelial type 1 cells (AEC1s) in the regeneration after ALI. CTGF initiates the phosphorylation of LRP6 by promoting the interaction between Krt5 and LRP6 of AEC2s, thus activating the Wnt signaling pathway, which is the molecular mechanism of CTGF promoting the proliferation of AEC2s subpopulation.

Conclusions
Our study verifies that CTGF promotes the repair and regeneration of alveoli after acute lung injury by promoting the proliferation of AEC2s subpopulation.
Phosphoflow cytometry to assess cytokine signaling pathways in peripheral immune cells: potential for inferring immune cell function and treatment response in patients with solid tumors
Authors: Toney Nicole J. , Schlom Jeffrey , Donahue Renee N. 
Published: September 2023

AbstractTumor biopsy is often not available or difficult to obtain in patients with solid tumors. Investigation of the peripheral immune system allows for in-depth and dynamic profiling of patient immune response prior to and over the course of treatment and disease. Phosphoflow cytometry is a flow cytometry‒based method to detect levels of phosphorylated proteins in single cells. This method can be applied to peripheral immune cells to determine responsiveness of signaling pathways in specific immune subsets to cytokine stimulation, improving on simply defining numbers of populations of cells based on cell surface markers. Here, we review studies using phosphoflow cytometry to (a) investigate signaling pathways in cancer patients’ peripheral immune cells compared with healthy donors, (b) compare immune cell function in peripheral immune cells with the tumor microenvironment, (c) determine the effects of agents on the immune system, and (d) predict cancer patient response to treatment and outcome. In addition, we explore the use and potential of phosphoflow cytometry in preclinical cancer models. We believe this review is the first to provide a comprehensive summary of how phosphoflow cytometry can be applied in the field of cancer immunology, and demonstrates that this approach holds promise in exploring the mechanisms of response or resistance to immunotherapy both prior to and during the course of treatment. Additionally, it can help identify potential therapeutic avenues that can restore normal immune cell function and improve cancer patient outcome.
Efficacy of the latest new stimulation patterns of spinal cord stimulation for intractable neuropathic pain compared to conventional stimulation: study protocol for a clinical trial
Authors: Tanei Takafumi , Maesawa Satoshi , Nishimura Yusuke , Nagashima Yoshitaka , Ishizaki Tomotaka , Ando Masahiko , Kuwatsuka Yachiyo , Hashizume Atsushi , Kurasawa Shimon , Saito Ryuta 
Published: September 2023

Abstract
Background
Spinal cord stimulation (SCS) is one of the neuromodulation therapies for chronic neuropathic pain. The conventional paresthesia-based SCS involves the application of tonic stimulation that induces a sense of paresthesia. Recently, new SCS stimulation patterns without paresthesia have been developed. Differential target multiplexed (DTM) stimulation and fast-acting subperception therapy (FAST) stimulation are the latest paresthesia-free SCS patterns.

Methods
A single-center, open-label, crossover, randomized clinical trial to investigate the superiority of SCS using the latest new stimulation patterns over conventional tonic stimulation for neuropathic pain is planned. This study consists of two steps: SCS trial (first step) and SCS system implantation (second step). In the SCS trial, participants will be randomly assigned to 4 groups receiving stimulation, including tonic, DTM, and FAST. Each stimulation will then be performed for 2 days, and a visual analog scale (VAS) for pain will be evaluated before and after each stimulation pattern. A stimulation-off period for 1 day is set between each stimulation pattern to wash out the residual previous stimulation effects. Pain improvement is defined as more than 33% reduction in the pain VAS. The primary analysis will compare pain improvement between the new stimulation patterns and the conventional tonic stimulation pattern in the SCS trial. The secondary outcomes will be evaluated as follows: (1) the relationships between causative disease and improvement rate by each stimulation pattern; (2) comparison of pain improvement between the DTM and FAST stimulation patterns in all cases and by causative disease; (3) changes in assessment items preoperatively to 24 months after the implantation; (4) preoperative factors associated with long-term effects defined as continuing for more than 12 months; and (5) adverse events related to this study 3 months after the implantation.

Discussion
This study aims to clarify the effectiveness of the latest new stimulation patterns compared to the conventional tonic stimulation. In addition, which stimulation pattern is most effective for which kind of causative disease will be clarified.

Trial registration
Japan Registry of Clinical Trials (jRCT) 1,042,220,094. Registered on 21 November 2022, and last modified on 6 January 2023. jRCT is an approved member of the Primary Registry Network of WHO ICTRP.
Enzymatic synthesis of novel pyrrole esters and their thermal stability
Authors: Hu Jingyi , Zhou Meng , Zhang Yujie , Zhang Xi , Ji Xiaoming , Zhao Mingqin , Lai Miao 
Published: September 2023

AbstractIn the present work a simple enzymatic approach (Novozym 435) for transesterification to synthesize pyrrole esters was reported. To generate the best reaction conditions, which resulted in the optimum yield of 92%, the effects of lipase type, solvent, lipase load, molecular sieves, substrate molar ratio of esters to alcohol, reaction temperature, reaction duration, and speed of agitation were evaluated. The range of alcohols was assessed under optimal circumstances. The spectrum observations conclusively demonstrated that the compounds could be generated with high yield under the circumstances utilized for synthesis. The odor characteristics of the pyrrolyl esters obtained were examined by gas chromatography–mass spectrometry-olfactometry (GC–MS–O). Among them, compounds of benzhydryl 1H-pyrrole-2-carboxylate (3j), butyl 1H-pyrrole-2-carboxylate (3k) and pentyl 1H-pyrrole-2-carboxylate (3l) present sweet and acid aroma. In addition, the thermal degradation process was further studied using the Py–GC/MS (pyrolysis–gas chromatography/mass spectrometry), TG (thermogravimetry), and DSC (differential scanning calorimeter) techniques. The outcomes of the Py–GC/MS, TG, and DSC techniques show that they have excellent thermal stability.
Schistosoma japonicum EKLF/KLF1 is a potential immune target to tackle schistosomiasis
Authors: Piao Xianyu , Jiang Ning , Liu Shuai , Duan Jiamei , dai Hang , Hou Nan , Chen Qijun 
Published: September 2023

Abstract
Background
Interruption of parasite reproduction by targeting migrating schistosomula is a promising strategy for managing schistosomiasis. Hepatic schistosomula proteins previously identified based on second-generation schistosome DNA sequencing were found to hold excellent potential for schistosomiasis japonica diagnosis and as vaccine candidates. However, there are still many unknown schistosomula proteins that warrant further investigations. Herein, a novel schistosomula protein, the Schistosoma japonicum erythroid Krüppel-like factor (SjEKLF/KLF1), was explored.

Methods
Sequence alignment was carried out to detect the amino acid sequence characteristics of SjEKLF. The expression profile of SjEKLF was determined by western blot and immunofluorescence analysis. Enzyme-linked immunosorbent assay was used to determine the antigenicity of SjEKLF in hosts. Mice immunised with recombinant SjEKLF were challenged to test the potential value of the protein as an immunoprotective target.

Results
SjEKLF is defined as EKLF/KLF1 for its C-terminal DNA-binding domain. SjEKLF is mainly expressed in hepatic schistosomula and male adults and located within the intestinal intima of the parasites. Notably, high levels of SjEKLF-specific antibodies were detected in host sera and SjEKLF exhibited outstanding sensitivity and specificity for schistosomiasis japonica immunodiagnosis but failed to distinguish between ongoing infection and previous exposure. In addition, SjEKLF immunisation reduced the infection in vivo, resulting in decreased worm and egg counts, and alleviated body weight loss and hepatomegaly in infected mice.

Conclusions
Overall, these findings demonstrate that SjEKLF is critical for the infection of S. japonicum and may be a potential target to help control S. japonicum infection and transmission.

Graphical Abstract
What can the citations of systematic reviews of ethical literature tell us about their use?—an explorative empirical analysis of 31 reviews
Authors: Nobile Hélène , Moshtaghin Natali Lilie Randjbar , Lüddecke Zoë , Schnarr Antje , Mertz Marcel 
Published: September 2023

Abstract
Background
Systematic reviews of ethical literature (SREL) aim at providing an overview of ethical issues, arguments, or concepts on a specific ethical topic. As SREL are becoming more common, their methodology and possible impact are increasingly subjected to critical considerations. Because they analyse and synthetise normative literature, SREL are likely to be used differently than typical systematic reviews. Still, the uses and the expected purposes of SREL were, to date, mainly theoretically discussed. Our explorative study aimed at gaining preliminary empirical insights into the actual uses of SREL.

Methods
Citations of SREL in publications, both scientific and non-scientific, were taken as proxy for SREL uses. The citations of 31 published SREL were systematically searched on Google Scholar. Each citation was qualitatively analysed to determine its function. The resulting categorisation of SREL citations was further quantitatively investigated to unveil possible trends.

Results
The analysis of the resulting sample of SREL citations (n=1812) showed that the selected SREL were mostly cited to support claims about ethical issues, arguments, or concepts, but also to merely mention the existence of literature on a given topic. In this sample, SREL were cited predominantly within empirical publications in journals from various academic fields, indicating a broad, field-independent use of such systematic reviews. The selected SREL were also used as methodological orientations either for the conduct of SREL or for the practical and ethically sensitive conduct of empirical studies.

Conclusions
In our sample, SREL were rarely used to develop guidelines or to derive ethical recommendations, as it is often postulated in the theoretical literature. The findings of this study constitute a valuable preliminary empirical input in the current methodological debate on SREL and could contribute to developing strategies to align expected purposes with actual uses of SREL.
Bilirubin potentiates etomidate-induced sedation by enhancing GABA-induced currents after bile duct ligation
Authors: Gao Hao , Zhao Qian , Song Jian-Gang , Hu Guo-Xia , Yu Wei-Feng , Jiao Ying-Fu , Song Jin-Chao 
Published: September 2023

Abstract
Objectives
Our previous clinical trial showed that etomidate requirements to reach an appropriate level of anesthesia in patients with obstructive jaundice were reduced, which means that these patients are more sensitive to etomidate. However, the mechanism is still not completely clear. The present study was aimed to investigate the mechanism by which bilirubin facilitates etomidate induced sedation.

Methods
A bile duct ligation (BDL) rat model was used to simulate obstructive jaundice. Anesthesia sensitivity to etomidate was determined by the time to loss of righting reflex (LORR). Intrathecal injection of bilirubin was used to test the effects of bilirubin on etomidate induced sedation. The modulating effects of bilirubin on GABA responses were studied using the whole-cell patch clamp technique.

Results
The time to LORR induced by etomidate was significantly decreased in the BDL groups (p < 0.05), and unconjugated bilirubin in serum and cerebrospinal fluid (CSF) were markedly increased (p < 0.05). The time to LORR induced by etomidate was decreased after intrathecal injection of bilirubin (p < 0.05). A bilirubin concentration of 1.0 μM increased the GABA-induced currents of rat cortical pyramidal neurons (p < 0.05). Furthermore, 1.0 μM bilirubin enhanced GABA-induced currents modulated by etomidate (p < 0.05).

Conclusions
Our results demonstrated that pathologic bilirubin in CSF could enhance etomidate induced sedation. The mechanism may be that bilirubin increase the GABA-induced currents of rat pyramidal neurons.
Territorial mimetics and room types: the spatial development of Swedish district courthouses 1970–2020
Authors: Kärrholm Mattias 
Published: September 2023

AbstractIn this article I investigate the spatial development of Swedish district courthouses and their different room types from 1970 to 2020, with attention to the specificities and commonalities with other building types. How have spatial form and use travelled between building types during this period, and how has this contributed to the recent, quite radical developments and transformation of the courthouse as a building type? In the article, I focus particularly on aspects relating to the architectural and spatial culture of citation, and on what I call territorial mimetics here. Based on a mixed-method approach, the study traces and discusses five spatial themes of typological change in district courthouses, trends that also can be seen as a part of deeper spatial and mimetic tendencies circulating in Swedish society during these decades. I conclude with a discussion of the specific mimetic style of the courthouse as characterised by an ongoing negotiation between type-specific rules and cross-type models.
Using social media marketing to pro-tourism behaviours: the mediating role of destination attractiveness and attitude towards the positive impacts of tourism
Authors: Alzaydi Zyad M. , Elsharnouby Mohamed H. 
Published: September 2023

AbstractSocial media has recently played a highly impactful role in communication. Specifically, these channels are vital and have a substantial influence on the attitudes, behavioural intentions, and actual behaviour of tourists. Previous research showed clearly that there is an increasing number of individuals who rely heavily on social media platforms for gathering information and gaining knowledge about various issues. The current research tries to show the impact of these platforms on citizens’—as individuals—attitudes and behaviours to support tourism activities. Specifically, the current study examines three research objectives: (1) The impact of social media marketing activities on pro-tourism behaviours. (2) The impact of social media marketing activities on both destination attractiveness and attitude towards the positive impacts of tourism. (3) The mediation role of destination attractiveness and attitude towards the positive impacts of tourism in the relationships between social media marketing activities and pro-tourism behaviours. We adapted the theory of uses and gratifications and the stimulus-organism-response framework in the current research, where social media marketing drives pro-tourism behaviours indirectly through both destination attractiveness and attitude towards the positive impacts of tourism. A survey of 200 Albaha residents was conducted. We have used AMOS to check the constructs’ validity and reliability and Hayes’s PROCESS macro to test the mediation. The findings show that (1) Social media marketing activities help in explaining why city citizens positively behave towards tourism activities in their city; (2) Sequentially both destination attractiveness and attitude towards the positive impacts of tourism partially mediate the relationships between social media marketing and pro-tourism behaviours. The current study contributes to the existing research through the elucidation of why residents behave to support tourism in their countries by using social media marketing activities. In addition, it adds a number of professional insights. For example, the admins of destination social media platforms should increase their efforts towards providing presence, conversation, interactivity, and sharing on these platforms.
Comparison of Honey & Povidone Iodine dressings in Post-Cesarean Surgical Site Wound Infection Healing
Authors: Shaikh Erum Majid , Pathan Sadia , Zuberi Bader Faiyaz , Rehman Memoona , Malik Shoaib 
Published: September 2023

Objective: To compare the healing of post-cesarean infected wounds by a combination of honey and povidone iodine & povidone-iodine alone dressings using ASEPSIS Score.
Method: This prospective randomised cohort study was carried out at at OBGYN Department Jinnah Postgraduate Medical Center from 1st August 2022 till 31st December 2022. Patients were randomly allocated into groups A and B. Group-A was dressed with honey povidone-iodine, while Group-B only had povidone-iodine. The ASEPSIS score was calculated in both groups on day fifth, 7th, 9th, and 10th day. Patients were followed till full recovery of the wound.
Results: A total of 70 women were included and equally allocated into two groups A & B (35 each). On day five mean ASEPSIS score in Group-A was 36.14 and that in Group- B was 37.74. No significant difference in scores were noted on day five [t (68) = -.753, p = .454] & day seven Group-A 28.63 vs Group-B 32.11 [t (68) = -1.302, p = .197]. Significant improvements in ASEPSIS scores were noted on days ninth & tenth. On day nine ASEPSIS score of Group-A was 21.54 and that of Group-B was 27.14 [t (68) = -2.056, p = .044]. On day tenth the mean ASEPSIS score of Group-A was 18.26, while that of Group-B was 23.86 [t (68) = -2.021, p = .047]. The mean time required for the wound to heal in Group-A and Group-B was eighteen & twenty-one days respectively.
Conclusion: Significant improvements in ASEPSIS score occurred with the use of honey with povidone-iodine combination.
doi: https://doi.org/10.12669/pjms.39.6.7499
How to cite this: Majid E, Pathan S, Zuberi BF, Rehman M, Malik S. Comparison of Honey & Povidone Iodine dressings in Post-Cesarean Surgical Site Wound Infection Healing. Pak J Med Sci. 2023;39(6):1803-1808. doi: https://doi.org/10.12669/pjms.39.6.7499
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Maternal parenting style and body mass index among 6–12-year-old girls in Saudi Arabia: A pilot study
Authors: Alabdulkarim Aljawharah , Binshaieg Lamia , Alrashood Ghadah , Alkhudhiri Shooq , Alqahtani Yasmeen , Benajiba Nada , Chavarria Enmanuel A. , Bernstein Joshua , Aboul-Enein Basil H. 
Published: September 2023

Background: An increasingly high prevalence of overweight and obesity exists among Saudi children. Parenting style may serve as a potential predictor in overweight and obesity. Aim: This study examines parenting style as a potential predictor relationship in overweight and obesity among Saudi children. Methods A cross-sectional study involving 92 paired girls and mothers was conducted to determine the association between maternal parenting styles and Saudi girls’ BMI. Findings: The results indicated that the most prevalent parenting style was the authoritative. Majority of students have a moderate obesogenic environment. However, it was not significantly correlated with students BMI. Also, parenting styles were not significantly correlated with an obesogenic environment. Conclusions: Factors other than maternal parenting styles such as environmental factors, families’ socioeconomic status, or cultural factors might be stronger contributors to an obesogenic environment. Larger heterogenous studies are warranted to explore children's BMI and parenting styles associations in Saudi Arabia.
A Rare Cause of Diarrhea: Intestinal Spirochetosis in an HIV-Positive Patient
Authors: Falgout Lacey , Kailas Shilpa , Sridhar Supraja , Loganantharaj Nisha 
Published: September 2023

ABSTRACT
Intestinal spirochetosis (IS) is a rare gastrointestinal infection with vague presenting symptoms. Diagnosis is confirmed histopathologically. Risk factors include homosexuality and HIV. Antibiotic treatment with metronidazole usually leads to resolution of symptoms. We present the case of a 56-year-old HIV-positive man with chronic, watery diarrhea who was diagnosed with IS. This case highlights the importance of considering IS in the differential in HIV-positive patients with nonspecific gastrointestinal symptoms after more common etiologies have been ruled out.
Acute Hemorrhagic Pancreatitis as a Rare Complication of Dengue Fever
Authors: Khataniar Himsikhar , Vellankal Shreya 
Published: September 2023

ABSTRACT
Dengue fever can lead to a range of symptoms, including severe manifestations such as dengue hemorrhagic fever. We report a rare case of severe dengue with hemorrhagic pancreatitis and pseudocyst formation in a 28-year-old woman. Initially presenting with fever, abdominal pain, and gastrointestinal symptoms, the patient developed worsening pain, vomiting, and an abdominal mass. Imaging revealed a pancreatic mass, and elevated amylase and lipase confirmed pancreatitis. Interventional radiology drained the hemorrhagic fluid from the suspected pseudocyst, leading to patient improvement and eventual discharge. This uncommon complication of acute hemorrhagic pancreatitis in dengue fever has not been previously documented.
Unexpected Drug-Induced Liver Injury Associated With MenoFit: A Synbiotic Menopause Supplement
Authors: Dadlani Apaar , Anudu Azubuogu , Marginean E. Celia 
Published: September 2023

ABSTRACT
MenoFit is a widely available over-the-counter synbiotic supplement, which is marketed for use in relieving menopausal symptoms. So far, there is no published data on liver injury because of its use. We present the first reported case of MenoFit-induced liver injury in a patient who presented with 1 week of jaundice and abnormal liver biochemical tests in the absence of other risk factors and negative comprehensive workup for known etiologies of liver disease.