bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and characterization of triclocarban degrading bacteria as plant growth promoter
Authors:   , Sipahutar Merry 
Published: September 2023

Triclocarban (TCC) is a widely used as antimicrobial agent in household products. High usage volume of TCC results in its accumulation in sludge and lead to contamination in environments. Application of biosolid in agricultural activities leads to contamination of TCC in agricultural areas. So. development of bioremediation technique for TCC is necessary to maintain quality of agricultural ecosystem. For that purpose, this research isolated 5 soil bacteria capable of TCC degradation and with plant-growth promoting (PGP) activities including Ochrobactrum sp. MC22, Ochrobactrum sp. MC35, Sphingobacterium sp. MC43, Pseudomonas sp. MS45, and Pseudomonas fluorescens MC46. Among them, Ochrobactrum sp. MC22 and P. fluorescens MC46 were selected for further studies. The results showed that Ochrobactrum sp. MC22 could degrade TCC under aerobic and anaerobic conditions. It has ability to degrade TCC in a wide range of concentrations from 0.16-30 mg L-1. The analysis of TCC degradation kinetics and degradation pathway revealed that chloroanilines are degradative intermediates, which could be completely metabolized and detoxified as shown by the toxicity test in pot soil experiment using 2 legume plants. In the case of P. fluorescens MC46, not only TCC degradation capability, degradation kinetics, and degradation pathway were analyzed, but it was also developed into a bacterial formula in order to improve its efficiency for TCC degradation and PGP. Moreover, its usage was proven in soil experiment with mung bean plants where the soil was contaminated with TCC. The bioaugmentation of P. fluorescens MC46 formula in to the contaminated soil not only reduced TCC by 74-76% of its initial concentration, mitigated TCC toxicity, promoted plant growth, but it also improved soil quality with increasing soil enzyme activities. In conclusion, this study demonstrated the potential use of Ochrobactrum sp. MC22 and P. fluorescens MC46 for bioremediation of soil contaminated with TCC as well as chloroanilines, while they could promote plant growth.
The relationship between geochemical parameters and the communities of arsenite-oxidizing bacteria in Rayong groundwater basin
Authors:   , Pipattanajaroenkul Phurinat 
Published: September 2023

As is one of toxic metalloid commonly found on the earth's surface. In groundwater, arsenite (As3+) and arsenate (As5+) are most predominant As species. Arsenite is more toxic than arsenate. As removal through bioremediation technique can be applied as an alternative method. This study aimed to investigate the community of arsenite-oxidizing bacteria in As-contaminated groundwater and to correlated them to the geochemical parameters. Nineteen groundwater samples were collected from Ban-khai and Muang districts, Rayong province, Thailand. The communities of arsenite-oxidizing bacteria were detected by using PCR-cloning-sequencing techniques, targeting aioA gene. The results showed that the detected arsenite-oxidizing bacteria were associated with α-, β-, and γ-Protebacteria. The γ-Protebacterial cluster was detected in groundwater with low to moderate As concentrations, while it was undetected in groundwater with high As concentration. PHREEQC geochemical model was used to identify the major As species in all groundwater samples. The predominant As species in most groundwater samples is arsenite (As3+) which presented in the form of H3AsO3. In addition, the statistical analysis demonstrated that the geochemical parameters affecting the distribution of arsenite-oxidizing bacteria in this study were Fe, Mn, As3+ and As5+. Fe, Mn, and As3+ showed the positive relationship to the β-Proteobacterial cluster, whereas As5+ positively correlated with α-Proteobacterial cluster. The knowledge gain from this study will help better understand the distribution of arsenite-oxidizing bacteria found in groundwater with a broad range of As concentrations.
Vector competence and correlation of midgut microbiota of Aedes albopictus for Chikungunya virus
Authors:   , Tuanudom Ranida 
Published: September 2023

Chikungunya virus (CHIKV) is an important mosquito-borne virus and transmission cycle of this virus involves mosquito vectors (Aedes albopictus and Aedes aegypti) and infected vertebrate hosts. The recently studies found that CHIKV outbreak in 2007 have been Ae. albopictus as an important vector which it was susceptible to genetic variation of CHIKV and induce to virus virulence. However, the study about vector competence for CHIKV in Thailand is limited. Moreover, the previous study indicates the impact of midgut microbiota of mosquito to viral infection. This study was conducted to examine the effects of CHIKV titers in blood meals on vector competence of Aedes albopictus (Diptera: Culicidae) and to investigate the correlation of midgut microbiota of Ae. albopictus. Six groups of Ae. albopictus were allowed to feed on different levels of CHIKV in the blood meals which were 106, 105, 104,103, 102 CID50/ml of CHIKV and the negative blood meal group. Body, legs and wings, and saliva samples from blood-fed mosquitoes were assayed for the presence of CHIKV by using immunocytochemistry staining on day 14 post blood feeding. Percent virus infection, dissemination, and transmission is defined as percent of blood-fed mosquitoes with virus in their bodies, legs and wings, and saliva, respectively. The percent infections were 83.3, 90, 100, 100, and 100%, the percent disseminations were 70.8, 86.7, 100, 90, and 98%, and the percent transmissions were 41.6, 70, 100, 90, and 82.4% after having been fed on 102, 103, 104, 105, 106 CID50/ml of CHIKV, respectively. This study suggested that Ae. albopictus are susceptible for CHIKV infection and efficient vectors for CHIKV transmission, and CHIKV titers in blood meals have effects on virus infection, dissemination, and transmission in Ae. albopictus or vector competence of this mosquito. For the bacterial isolation and identification, the midguts were cultured and 16s rRNA gene were analyzed followed by blast to gene bank database. The dominating bacterial genus was Micrococcus in infected mosquitoes after fed on CHIKV 102 CID50/ml, and it was significantly difference from the non-infected mosquitoes (P<0.04). In addition, Staphylococcus was the dominating bacterial genus in laboratory mosquitoes and it was significantly difference from field mosquitoes (P<0.0007). The correlation between midgut microbiota and CHIKV infection was not clearly indicated from this study.
Theoretical studies on electronic properties of curved graphene quantum dots and lithium adsorption on graphene quantum dots
Authors:   , Pattarapongdilok Naruwan 
Published: September 2023

Graphene quantum dots (GQDs), with their attractive properties, are of interest to apply for electronic devices. Therefore, this dissertation aimed to investigate the electronic of curved graphene quantum dots (CGQDs) and the adsorption of lithium on GQDs. For the first part, two shapes of flat GQDs, rhomboidal (RGQDs) and hexagonal (HGQDs), were modified to make CGQDs with different folding axes and angles. Stabilities and electronic properties of CGQDs were studied using PBE/DNP. The results showed that the deformation energies of GQDs depend on sizes and folding axes but not their shapes. HOMO-LUMO gap variations, both widening and narrowing the gap, upon folding were observed, and can be explained by orbital interactions. In the second study, three sizes of GQDs, coronene, circumcoronene, and circumcircumcoronene were allowed to be adsorbed by lithium ions and atoms at various positions of GQDs. Adsorption positions and binding energies were determined using M06-2X/6-31g(d). The results indicated that lithium ion and atom could bind better at the edge of GQDs. The adsorption between lithium ion and coronene showed the highest binding affinity of -135.073 kcal/mol. Moreover, the size increment of GQDs raises the binding energy, except Li+ absorption on GQDs with -1 charge. Preferred adsorption positions of lithium ions and atoms on GQDs can be described by the total charge of six-membered ring. In addition, binding affinities of one-Li-ion system on GQDs are larger than two-Li-ion system. For two Li-ion system, the binding energy on GQDs also depends on Li ion-Li ion repulsion.
Prevalence Of Chronic Temporomandibular Disorder Pain And Comorbidities In A Group Of Thai Patient: A Cross Sectional Study
Authors:   , Nguyen Tra 
Published: September 2023

Objectives: 1. to investigate the prevalence of chronic temporomandibular disorder (TMD) and its comorbidities in a group of Thai patients; 2. to evaluate the relationship between pain parameters (pain duration, pain intensity, and pain frequency) with the number of comorbidities and widespread pain index (WPI); 3. to assess the relationship between the presence of chronic pain with familial history in TMD pain patients. Methods: A cross-sectional study was undertaken of 351 outpatients. Patients were given an interviewer-administered questionnaire which contained four parts (demographic data, pain parameters, WPI and comorbidities diagnosis). Diagnosis was made in accordance with Diagnostic Criteria for Temporomandibular Disorder. Spearman rank correlation coefficient linear regression, and Chi-square test were used, with a significance level of p ≤ 0.05. Results: 25% reported chronic pain. 82% of chronic pain patients had at least one comorbidity. The number of comorbidities was associated with pain duration (p = 0.02); WPI was related to pain duration and pain intensity (p < 0.001). The presence of chronic TMD pain was not related to familial history (OR = 1.31; 95% CI = 0.78 – 2.21). Conclusions: The presence of comorbidity was prevalent in chronic TMD pain patients. To predict the presence of comorbidities or spreading pain presence, pain duration is a feasible factor. Pain intensity can be used to predict the presence of spreading pain, but its reliability is not clear.
Metallosalen-based polymers for reduction of carbon dioxide
Authors:   , Kaewyai Cherawat 
Published: September 2023

In this research, a series of novel asymmetric copper- and nickel-salens bearing thiophene-based substituents were successfully synthesized and characterized by nuclear magnetic resonance spectroscopy and mass spectrometry. Homogeneous electrocatalytic activities of these monomers toward reduction of CO2 were investigated using cyclic voltammetry technique. Electrochemical studies showed these salen monomers are able to serve as catalysts in the electrochemical reduction of CO2 due to the significantly increase of the current observed under CO2-saturated condition, compared with those found under the N2-saturated one. Results also revealed that insertion of the carbon-carbon triple-bond between the thiophene-based substituents and the salen core led to the lower required reduction potential, while the additional thiophene rings did not significantly influence in this aspect. Furthermore, addition of 3% of H2O as a proton source resulted in even higher current enhancement in the reduction of CO2. Electropolymerization of the target monomers through their thiophene-based units gave desirable metallosalen-based polymers in most cases. In the polymerization process, results showed that the introduction of bithiophenyl rings led to the lower required oxidation potential, while the derivatives containing carbon-carbon triple-bond spacers required higher oxidation potential than those having the thiophene-based substituents directly linked to the salen cores. However, further optimization of the polymerization condition is required to obtain more efficient films for the reduction of CO2.
Effect of corrosion inhibitors in lubricant for electronic industry
Authors:   , Maipul Natclitta 
Published: September 2023

The selected functional polymers and fatty esters of poly)1-butene pyrrolidone) (PP) and propylene glycol monostearate (PG) were investigated and applied to use as the corrosion inhibitors for protecting material removal during read/write head lapping processes in hard disk drive (HDD) production. Potentiodynamic polarization measurement was used to study their corrosion behaviors against antiferromagnetic materials (IrMn) which are the most sensitive materials in the head structure. Corrosion rate of PP- and PG-modified lubricants were 0.086 and 0.084 Å/min, respectively, while standard lubricant was 0.111 Å/min. Moreover, the leaching out of metallic atoms was investigated by using inductively couple plasma mass spectroscopy (ICP-MS). Leaching out of metallic atoms was in range 3-7 µg/L for PP- and PG-modified lubricant but, standard lubricant was 4-15 µg/L. The results showed that corrosion rate can be reduced compared to the standard glycol-based lubricant. The conductivity and acid-base properties of the modified lubricants were also measured and analyzed. Scanning electron microscopy (SEM) showed that the protection of those inhibitors came from the formation of protective thin layer over the surface by their functional groups. Furthermore, the electrical performance parameters from quasi static test (QST) and lapping test indicated a better lapping performance, without losing quality and electrical performance of the slider. We propose that PG and PP are the 1st and 2nd choice of additional additives in corrosion protection of IrMn material in HDD slider fabrication process.
Antibacterial agents from Dracaena cochinchinensis (Lour.) S.C.Chen and Eleutherine americana (Aubl.) Merr. Ex K. Heyne
Authors:   , Ruga Ritbey 
Published: September 2023

Fifteen compounds including two new anthraquinones (118-119), two naturally occurring new compounds (116-117) and eleven known compounds were successfully isolated from the CH2Cl2 extracts of Dracaena cochinchinensis and Eleutherine americana i.e. dihydrochalcones (5 and 23), pterostilbene (27), homoisoflavanones (15 and 82-84), naphthalenes (85 and 116), pyranonaphthoquinone (86) and anthraquinones (99-100 and 117-119). Among isolated compounds, (+)-eleutherin (86) was the most potent antibacterial agent against Propionibacterium acnes KCCM 41747, Staphylococcus aureus ATCC 25923, Streptococcus sobrinus KCCM 11898, Streptococcus mutans ATCC 25175 and Salmonella typhi ATCC 422 with inhibition zones of 14.7 to 18.0 mm and MIC values of 0.24 to 7.8 µM. Compound 86 showed bacteriostatic effect against P. acnes while bactericidal agent was observed by this compound against other tested bacteria. The combination of chloramphenicol and 86 demonstrated synergistic effect against all tested bacteria. Moreover, anti-allergic activity of nine compounds on the release of β-hexosaminidase in rat basophil leukemia (RBL-2H3) cells by calcium ionophore A23178-induced stimulation were investigated. Loureirin B (5) showed the strongest activity followed by 23 and 27 with IC50 values of 7.8, 15.8 and 17.5 µM, respectively. For anticancer activity, 86 suppressed the growth of HeLa, HCT116 and HepG2 cancer cell lines with IC50 values of 0.3, 4.6 and 6.7 µM, respectively. Compound 86 displayed twice more toxic than positive control (5-fluorourasil) against HeLa cells. The suppression against the cell was also shown by 23 and 27 with IC50 value of 1.4 µM.
Development of hydrogen embrittlement resisted pd-cu alloy membrane
Authors:   , Chukiatthai Ratchaneekorn 
Published: September 2023

The development of PdCu alloy membranes that can be used in hydrogen separation at low working temperature (<300°C) without hydrogen embrittlement problem is a very attractive topic for the petrochemical industry. Pd–Cu membranes have been prepared by electroless plating and electroplating method on stainless steel support. The compositions of the PdCu alloy membranes were related to both the metal deposition rates. The appropriate annealing temperature of Pd–Cu membranes was found at 500 °C in argon atmosphere for 24 hours to form a complete alloy material. After thermal annealing, the Cu atom was distributed in Pd alloy membrane. The surface morphology, compositions, and crystallinity of the PdCu alloy membranes were characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). The best Pd contents in PdCu alloy membrane were about 84 and 75 %at. The hydrogen flux through the PdCu alloy membrane was measured at temperature 150 to 300 °C and differential pressure 0.5 to 2.5 bar for membrane stability. The hydrogen flux increased with increasing temperature and pressure. Adding Cu atoms in Pd can reduce strain in Pd lattice structure. Both PdCu alloy membrane can be operated at the lower temperature than a critical temperature of pure Pd membrane without hydrogen embrittlement.
Characteristics and factors affecting contraceptive utilization in premarital sexual relationship among unmarried youths in rural Yangon Myanmar
Authors:   , Lwin Hnin 
Published: September 2023

Background: Globally, youths are more sexually active compared to other age groups of the population. Several studies showed premarital sexual intercourse appears all regions around the world. The objective of this study was to access characteristics and factors affecting actual and intended contraceptive utilization in premarital sexual relationship among unmarried youths in rural Yangon, Myanmar.

Methods: A quantitative cross sectional descriptive study was done with 270 unmarried youths with the age of 15-24 who are residing in a rural township of Yangon region. Data was collected by using combined interviewer-administered questionnaire and self-administered questionnaire between September and October 2017. Self-administered questionnaire was prepared for sensitive questions on sexual experiences.

Result: The finding showed 44% of sexually active unmarried youths used at least one modern contraceptive method in their latest sexual intercourse. Always use at least one modern contraceptive method was found only in 17.6% of sexually active unmarried youths. 44.8% of unmarried youths intended to use only modern contraceptive method while 8.1% intended to use both modern and traditional contraceptive methods in the future. The final model of multiple logistic regression revealed that there was no association between any independent variables and actual use of contraception in the latest sexual intercourse and always use in the life-time. Intended use of contraception in the future showed significant positive associations with age group of respondents (p value<0.001), sex (female, p value=0.044), having own income (p value=0.013), level of attitude (p value=0.034), level of belief (p value=0.002), ever heard about contraception (p value= 0.003), easy availability of contraception when needed (p value< 0.001), same gender service provider (p value= 0.003) and experience on sexual intercourse (p value< 0.001)in the final model of regression.

Conclusion: Low prevalence of contraceptive utilization, and low level of knowledge about contraception especially for emergency contraceptive pill and IUD were found among unmarried rural youths. Delivering more information about contraceptive methods through comprehensive sexual and reproductive health education in middle and high schools, Facebook, mobile application and edutainment program in TV channels should be implemented. Furthermore, unbiased and respectful care should be provided to both married and unmarried youths who seek reproductive health care or contraceptive service at the public or private or NGO clinic. Youth information centers or youth-friendly clinic should also be provided for youths who live in rural areas of Yangon region.
Knowledge attitude and practice (kap) of long term care services for the elderly among trained caregivers in Sisaket province Thailand
Authors:   , Doungjan Kamonthip 
Published: September 2023

This study aimed to assess knowledge, attitudes, and practices of long term care for the elderly among caregivers and to identify association between socio-demographic factors, knowledge, attitudes, and practices of long term care for the elderly among caregivers in Sisaket province.

A cross sectional descriptive study with a self-administered questionnaire was conducted among 209 caregivers in Sisaket province from March to July 2018. Analysis of the variables was done using univariate and bivariate at 95% confident interval by SPSS Version 16.

The result founded out of 209 caregivers, majority of them (93.8%) were female, whereas 6.2% were male. Twenty-three percent of caregiver are relatives with the elderly in their responsibility. The Majority of respondents had a high level of knowledge in elderly care (90%), 80.4% were having neutral attitude towards elderly care in long term care and 53.6 % had the moderate practice level in long term care. For association between variables found that number of home bound, caregivers’ relationship with elderly and attitude level were significantly associated with practices of long term care for the elderly among caregivers (p-value <0.05).
Internet addiction and health problems through smartphone among international students in a university in Bangkok Thailand : online questionnaire
Authors:   , Phromsiri Supattra 
Published: September 2023

The increase in Internet use through smartphones, particularly among the generation Y group, has brought about several health challenges in today’s society. Few studies have investigated the issue of Internet addiction (IA) through the smartphone in Bangkok to date. This cross-sectional study aimed to assess IA level and to determine Internet-related health problems among international program students in a university in Bangkok. Further, this study sought to determine the association between level of smartphone IA and health problems. Data regarding the pattern of Internet use, IA level, and related health problems were collected via a self-administered online questionnaire. Validity and reliability of the instrument were evaluated and deemed acceptable for research prior to use. Young’s Internet Addiction Test (IAT), a standardized instrument, was used to measure the IA level (Cronbach’s alpha = 0.89). Data were analyzed using descriptive statistics, Chi-square, and Fisher's exact test. Of the 351 participants (mean age 26.8 years, SD ± 7.1), 44% were in the level of mild IA, 34% in the moderate level, and 3% severe level. Overall, eye strain was most reported by the participants (73%). More than one-third of all participants said having had experienced headaches (41%), inadequate sleep (55%), and cramped hand/wrist/arm (40%); accidents were the least reported (21%). IA level was significantly associated with the occurrence of eye strain, inadequate sleep, and hand/wrist/arm cramping. The evidence based of this study regarding Internet addiction and health problems may point to strategic plan of prevention and intervention for implementing policy in the future.