bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling nanoscale optical signatures of cytokine-induced β-cell dysfunction
Authors: Pugliese Licia Anna , De Lorenzi Valentina , Bernardi Mario , Ghignoli Samuele , Tesi Marta , Marchetti Piero , Pesce Luca , Cardarelli Francesco 
Published: August 2023

AbstractPro-inflammatory cytokines contribute to β-cell failure in both Type-1 and Type-2 Diabetes. Data collected so far allowed to dissect the genomic, transcriptomic, proteomic and biochemical landscape underlying cytokine-induced β-cell progression through dysfunction. Yet, no report thus far complemented such molecular information with the direct optical nanoscopy of the β-cell subcellular environment. Here we tackle this issue in Insulinoma 1E (INS-1E) β-cells by label-free fluorescence lifetime imaging microscopy (FLIM) and fluorescence-based super resolution imaging by expansion microscopy (ExM). It is found that 24-h exposure to IL-1β and IFN-γ is associated with a neat modification of the FLIM signature of cell autofluorescence due to the increase of either enzyme-bound NAD(P)H molecules and of oxidized lipid species. At the same time, ExM-based direct imaging unveils neat alteration of mitochondrial morphology (i.e. ~ 80% increase of mitochondrial circularity), marked degranulation (i.e. ~ 40% loss of insulin granules, with mis-localization of the surviving pool), appearance of F-actin-positive membrane blebs and an hitherto unknown extensive fragmentation of the microtubules network (e.g. ~ 37% reduction in the number of branches). Reported observations provide an optical-microscopy framework to interpret the amount of molecular information collected so far on β-cell dysfunction and pave the way to future ex-vivo and in-vivo investigations.
Expression status of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 in patients with colorectal cancer
Authors: Hussein Neveen A. , El Sewedy Shehata M. , Zakareya Mohamed M. , Youssef Engy A. , Ibrahim Fawziya A. R. 
Published: August 2023

AbstractColorectal cancer (CRC) poses a significant burden on both the healthcare systems as well as individuals. The high mortality rate of CRC may be attributed to its metastatic potential, heterogeneity, and delayed diagnosis. CircRNAs are an essential class of regulatory RNAs that play significant roles in cancers. This study aimed to detect the expression status of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 in patients with CRC. This study included 50 CRC patients, 30 individuals with colorectal diseases (non-cancer), and 20 healthy volunteers. By using real-time PCR, the relative expression of circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 was determined in the collected blood samples. In addition, ECLIA was used to quantify carcinoembryonic antigen (CEA) level. All circRNAs expression and CEA levels were significantly up-regulated in cancer patients (CRC, colon, rectum) as compared to healthy controls, except circ-SMARCA5. Moreover, there was a significant up-regulation of circRNAs in most non-cancer patients (UC, polyp, piles). Insignificant upregulation was observed in circRNAs and CEA when comparing cancer with non-cancer patients. No correlations were found between the studied parameters and most clinicopathological characteristics of cancer and non-cancer patients. Circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 were differentially expressed in patients with CRC as well as in non-cancer patients. Circ-SMARCA5 and circ-NOL10 may act as tumor suppressors, while circ-LDLRAD3 and circ-RHOT1 may be oncogenes. Circ-SMARCA5, circ-NOL10, circ-LDLRAD3, and circ-RHOT1 could be promising markers for the early detection of CRC.
Body temperature as a predictor of mortality in COVID-19
Authors: Uchiyama Shuhei , Sakata Tomoki , Tharakan Serena , Ishikawa Kiyotake 
Published: August 2023

AbstractIt remains uncertain if body temperature (BT) is a useful prognostic indicator in coronavirus disease 2019 (COVID-19). We investigated the relationship between BT and mortality in COVID-19 patients. We used a de-identified database that prospectively collected information from patients screened for COVID-19 at the Mount Sinai facilities from February 28, 2020 to July 28, 2021. All patients diagnosed with COVID-19 that had BT data were included. BT at initial presentation, maximum BT during hospitalization, comorbidity, and vaccination status data were extracted. Mortality rate was assessed as a primary outcome. Among 24,293 cases, patients with initial BT below 36 °C had higher mortality than those with BT of 36–37 °C (p < 0.001, odds ratio 2.82). Initial BT > 38 °C was associated with high mortality with an incremental trend at higher BT. In 10,503 in-patient cases, a positive association was observed between mortality and maximum BT except in patients with BT < 36 °C. Multiple logistic regression analyses including the comorbidities revealed that maximum BT was an independent predictor of mortality. While vaccination did not change the distribution of maximum BT, mortality was decreased in vaccinated patients. Our retrospective cohort study suggests that high maximum BT is an independent predictor of higher mortality in COVID-19 patients.
Study on the influence of urban tree canopy on thermal environment in Luoping County
Authors: Dai Ji-ping , Qin Ji-zheng , Zhou Tianyu , Qin Xiao-jian , Zhu Kun , Peng Jian-song 
Published: August 2023

AbstractUrban forest is an integral part of the complex urban ecosystem, and tree canopy plays a key role in improving urban climatic environment. Urban Tree Canopy (UTC) is strongly linked to urban thermal environment and living quality of residents. In this study, Luoping County, a mountainous county in southwest China, was selected as the study area to uncover the inner connections between tree canopy and thermal environment, and provide relevant scientific references for the construction of livable forest cities in similar areas. Through eCongnition Developer, ENVI and ArcGIS software, the distribution of Land Surface Temperature (LST) and land cover types in the study area was extracted, 63 patches with super-large and extra-large tree canopy coverage selected, to explore the regulatory effect of UTC patches on urban thermal environment based on SPSS software. Results showed that the highest LST in the research area was 37.63 ℃, the lowest 24.73 ℃, and the average 30.83 ℃. Among the land cover types, the area of buildings and impervious surfaces was 1615.71 hm2, accounting for 55.76% of the total study area, which was the largest proportion and with widespread distribution; the area of grassland and water body was 57.48 hm2 and 12.35 hm2, respectively, taking up 1.98% and 0.43%, with a smaller proportion. Mean LST: impervious surface > bare land > grassland > tree canopy > water body. By increasing the area and perimeter of the patch covered by tree canopy, the cooling rate of the patch can be increased while the temperature inside the patch can be reduced. The relationship between the area and cooling rate is closer than that between perimeter and cooling rate. The increase of perimeter has a stronger alleviation effect on the internal temperature of the patch, whereas, the increase of area has a weaker effect in this respect.
Atlatl use equalizes female and male projectile weapon velocity
Authors: Bebber Michelle R. , Buchanan Briggs , Eren Metin I. , Walker Robert S. , Zirkle Dexter 
Published: August 2023

AbstractThe atlatl is a handheld, rod-shaped device that employs leverage to launch a dart, and represents a major human technological innovation. One hypothesis for forager atlatl adoption over its presumed predecessor, the thrown javelin, is that a diverse array of people could achieve equal performance results, thereby facilitating inclusive participation of more people in hunting activities. We tested this hypothesis via a systematic assessment of 2160 weapon launch events by 108 people who used both technologies. Our results show that, unlike the javelin, the atlatl equalizes the velocity of female- and male-launched projectiles. This result indicates that a javelin to atlatl transition would have promoted a unification, rather than division, of labor. Moreover, our results suggest that female and male interments with atlatl weaponry should be interpreted similarly.
Congruence in European and Asian perception of Vietnamese facial attractiveness, averageness, symmetry and sexual dimorphism
Authors: Pavlovič Ondřej , Fiala Vojtěch , Kleisner Karel 
Published: August 2023

AbstractAttractiveness is a proposed universal cue to overall biological quality. Nonetheless, local raters and raters of the same ethnicity may be more accurate in assessing the cues for attractiveness than distant and unfamiliar raters. Shared ethnicity and shared environment may both affect rating accuracy: our aim was to compare their relative influence. Therefore, we photographed young Vietnamese participants (N = 93, 33 women) from Hanoi, Vietnam. The photographs were rated by Czechs, Asian Vietnamese, and Czech Vietnamese (raters of Vietnamese origin who lived in Czechia for all or most of their life). Using geometric morphometrics, we measured facial shape cues to biological quality: averageness, asymmetry, and sexual dimorphism. We expected that Vietnamese raters residing in Czechia and Vietnam would agree on perceived attractiveness and use shape-related facial cues to biological quality better than Czech European raters, who are less familiar with East Asians. Surprisingly, mixed-effect models and post hoc comparisons identified no major cross-group differences in attributed attractiveness and path analyses revealed that the three groups based their rating on shape-related characteristics in a similar way. However, despite the considerable cross-cultural agreement regarding perceived attractiveness, Czech European raters associated attractiveness with facial shape averageness significantly more than Vietnamese raters.
Low-density granulocytes display immature cells with enhanced NET formation in people living with HIV
Authors: Park Juwon , Dean Logan S. , Heckl Jack , Gangcuangco Louie Mar , Pedro Te-Kie , Tallquist Michelle D. , Seto Todd B. , Shiramizu Bruce , Chow Dominic C. , Shikuma Cecilia M. 
Published: August 2023

AbstractWhile the protective role of neutrophil extracellular traps (NETs) in limiting human immunodeficiency virus (HIV) spread to susceptible cells has been documented, there is comparatively little insight into whether NET formation is harmful in people living with HIV (PLWH). To gain insight into neutrophil dysregulation and the pathological role of NETs in HIV, we examined expressions of NET-associated markers [cell-free DNA (cfDNA) and citrullinated histone H3 (CitH3)] in the plasmas from a cohort of the Hawaii Aging with HIV-cardiovascular and HIV-seronegative (HIV−) individuals. In a subset of participants, circulating low-density granulocyte (LDG) levels and their maturation and activation status were analyzed via flow cytometry. We demonstrated higher plasma levels of CitH3 in PLWH compared to HIV- individuals. LDGs from PLWH had heightened CD66b, but reduced CD16 expression. The percentages and counts of CD10+ LDGs were significantly decreased in PLWH. In addition, the CD16Lo LDG subsets were enriched in PLWH, compared to HIV− group, indicating that immature LDGs are increased in PLWH. Moreover, LDGs from PLWH exhibited significantly higher NET forming capacity. In summary, our study presents evidence that LDGs from PLWH on ART display an immature and altered phenotype with increased NET formation. Among PLWH, plasma NET levels as well as LDG parameters correlated with blood markers for inflammation and coagulation, suggesting that neutrophil activation and NETs may exert proinflammatory and coagulation effects. Our data provide insights into the pathologic role of LDGs at least in part mediated through NET formation in PLWH.
Characterization of bacterial community in tobacco leaves at flue-curing and redrying processing stages
Authors: Yang Yue , Xu Ruyan , Yang Mengmeng , Xu Qiang , Miao Chenlin , Guo Jianhua , Mou Wenjun , Du Hang , Wei Gang , Hu Liwei , Hu Zongyu 
Published: August 2023

AbstractDuring the processing of tobacco leaves, flue-curing and redrying can affect the structure of bacterial community, having an effect on the aging quality of tobacco leaves. In order to characterize the effects of flue-curing and redrying on the bacterial community of tobacco leaves, the bacterial community of samples at different processing stages (before flue-curing, after flue-curing, before redrying and after redrying) was analyzed using Illumina sequencing. A total of 33 phyla, 79 classes, 195 orders, 344 families, 826 genera and 7922 ASVs were obtained from 36 samples. There was no significant difference in the core bacterial groups of tobacco leaf at four processing stages.Proteobacteriadominated at the phylum level.Sphingomonas,PseudomonasandMethylobacteriumwere the main genera shared by all samples. The functional prediction by PICRUSt showed an increase in the relative abundance of pathway related to metabolism after flue-curing and pathway related to environmental information processing after redrying. This study, we analyzed the changes of bacterial community and structural composition of tobacco leaves from flue-curing to redrying, and found that flue-curing had a greater effect on the microbial community than redrying. This is conducive for the exploration of microbial resources and improvement of tobacco leaf quality.
Electrocoagulation/flotation process for removing copper from an aqueous environment
Authors: Kashi Giti 
Published: August 2023

AbstractThe presence of copper in aqueous environments such as drinking water has led to several environmental effects, such as flavor and odor. The increase in Cu levels in ground and surface water has been mainly attributed to anthropogenic and natural sources. Consequently, this applied-analytical study aimed to investigate copper removal from urban drinking water through batch reactor electrocoagulation/flotation (ECF) with aluminum electrodes. The copper removal efficiency was evaluated under various operating conditions of current density (0.8–2.4 mA/cm2), initial concentration (1–100 mg/L), pH (3.5–10.5), and time (10–30 min). Cu was determined using the method outlined in the standard procedures (3500-Cu B at 4571 nm). The results indicated that increasing the current density from 0.8 to 2.4 mA/cm2and the reaction time from 10 to 30 min improved Cu+2removal efficiency (from 95 to 100%). In addition, the results demonstrated that Cu+2reduction is 100% with an initial concentration of 100 mg/L, a pH of 7.5, a reaction time of 30 min, and an anode current density of 2.4 mA/cm2. The Taguchi method results for copper removal efficiency show that reaction time is the most significant variable. Furthermore, Cu removal kinetics models in an ECF reactor are second-order (R2 > 0.92). The Cu removal in the ECF reactor is due to redox and adsorption. Moreover, the operational costs of Cu treatment with Al electrode pairs are estimated to range from 8857 and 9636 Rial/kg of Cu removed. Thus, it can be concluded that the ECF process is very efficient in removing Cu from aqueous environments under optimum conditions.
Metamaterial inspired electromagnetic bandgap filter for ultra-wide stopband screening devices of electromagnetic interference
Authors: Al-Hasan Muath , Alibakhshikenari Mohammad , Virdee Bal S. , Sharma Richa , Iqbal Amjad , Althuwayb Ayman A. , Falcone Francisco 
Published: August 2023

AbstractPresented here is a reactively loaded microstrip transmission line that exhibit an ultra-wide bandgap. The reactive loading is periodically distributed along the transmission line, which is electromagnetically coupled. The reactive load consists of a circular shaped patch which is converted to a metamaterial structure by embedded on it two concentric slit-rings. The patch is connected to the ground plane with a via-hole. The resulting structure exhibits electromagnetic bandgap (EBG) properties. The size and gap between the slit-rings dictate the magnitude of the reactive loading. The structure was first theoretically modelled to gain insight of the characterizing parameters. The equivalent circuit was verified using a full-wave 3D electromagnetic (EM) solver. The measured results show the proposed EBG structure has a highly sharp 3-dB skirt and a very wide bandgap, which is substantially larger than any EBG structure reported to date. The bandgap rejection of the single EBG unit-cell is better than − 30 dB, and the five element EBG unit-cell is better than − 90 dB. The innovation can be used in various applications such as biomedical applications that are requiring sharp roll-off rates and high stopband rejection thus enabling efficient use of the EM spectrum. This can reduce guard band and thereby increase the channel capacity of wireless systems.
Immunogenic cell death-based prognostic model for predicting the response to immunotherapy and common therapy in lung adenocarcinoma
Authors: Zhou Xiang , Xu Ran , Lu Tong , Wang Chenghao , Chang Xiaoyan , Peng Bo , Shen Zhiping , Yao Lingqi , Wang Kaiyu , Xu Chengyu , Shi Jiaxin , Zhang Ren , Zhao Jiaying , Zhang Linyou 
Published: August 2023

AbstractLung adenocarcinoma (LUAD) is a malignant tumor in the respiratory system. The efficacy of current treatment modalities varies greatly, and individualization is evident. Therefore, finding biomarkers for predicting treatment prognosis and providing reference and guidance for formulating treatment options is urgent. Cancer immunotherapy has made distinct progress in the past decades and has a significant effect on LUAD. Immunogenic Cell Death (ICD) can reshape the tumor’s immune microenvironment, contributing to immunotherapy. Thus, exploring ICD biomarkers to construct a prognostic model might help individualized treatments. We used a lung adenocarcinoma (LUAD) dataset to identify ICD-related differentially expressed genes (DEGs). Then, these DEGs were clustered and divided into subgroups. We also performed variance analysis in different dimensions. Further, we established and validated a prognostic model by LASSO Cox regression analysis. The risk score in this model was used to evaluate prognostic differences by survival analysis. The treatment prognosis of various therapies were also predicted. LUAD samples were divided into two subgroups. The ICD-high subgroup was related to an immune-hot phenotype more sensitive to immunotherapy. The prognostic model was constructed based on six ICD-related DEGs. We found that high-risk score patients responded better to immunotherapy. The ICD prognostic model was validated as a standalone factor to evaluate the ICD subtype of individual LUAD patients, which might contribute to more effective therapies.
Undrained stability of braced excavations in clay considering the nonstationary random field of undrained shear strength
Authors: Tanapalungkorn Weeradetch , Yodsomjai Wittawat , Keawsawasvong Suraparb , Nguyen Thanh Son , Chim-Oye Weeraya , Jongpradist Pornkasem , Likitlersuang Suched 
Published: August 2023

AbstractThe basal heave stability of supported excavations is an essential problem in geotechnical engineering. This paper considers the probabilistic analysis of basal heave stability of supported excavations with spatially random soils by employing the random adaptive finite element limit analysis and Monte Carlo simulations to simulate all possible outcomes under parametric uncertainty. The effect of soil strength variability is investigated for various parameters, including the width and depth of the excavation ratio, strength gradient factor, and vertical correlation length. Probabilistic basal stability results have also been employed to determine the probability of design failure for a practical range of deterministic factors of safety. Considering probabilistic failure analysis, the more complete failure patterns caused by the various vertical correlation length would decrease the probability of design failure. There are different tendencies between the probability of design failure at the same safety factor with various vertical correlation lengths. These results can be of great interest to engineering practitioners in the design process of excavation problems.
Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice
Authors: Park Cana , Hahn Oliver , Gupta Shweta , Moreno Arturo J. , Marino Francesca , Kedir Blen , Wang Dan , Villeda Saul A. , Wyss-Coray Tony , Dubal Dena B. 
Published: August 2023

AbstractPlatelet factors regulate wound healing and can signal from the blood to the brain1,2. However, whether platelet factors modulate cognition, a highly valued and central manifestation of brain function, is unknown. Here we show that systemic platelet factor 4 (PF4) permeates the brain and enhances cognition. We found that, in mice, peripheral administration of klotho, a longevity and cognition-enhancing protein3–7, increased the levels of multiple platelet factors in plasma, including PF4. A pharmacologic intervention that inhibits platelet activation blocked klotho-mediated cognitive enhancement, indicating that klotho may require platelets to enhance cognition. To directly test the effects of platelet factors on the brain, we treated mice with vehicle or systemic PF4. In young mice, PF4 enhanced synaptic plasticity and cognition. In old mice, PF4 decreased cognitive deficits and restored aging-induced increases of select factors associated with cognitive performance in the hippocampus. The effects of klotho on cognition were still present in mice lacking PF4, suggesting this platelet factor is sufficient to enhance cognition but not necessary for the effects of klotho—and that other unidentified factors probably contribute. Augmenting platelet factors, possible messengers of klotho, may enhance cognition in the young brain and decrease cognitive deficits in the aging brain.
Unmanned aerial vehicle orthogonal laser localization by Gaussian mixture model‐based map representation
Authors: Wan Zeyu , Jiang Changjian , Zhang Yu 
Published: August 2023

AbstractLocalization is a core problem in mobile robot navigation. Simultaneous localization and mapping (SLAM) costs much for an unmanned aerial vehicle (UAV). This research aims to design an orthogonal laser scan device for localization and to save computation costs. Based on disturbance analysis, residual influences on sensor state are quantitative, and they are related to uncertainty and sensitivity. This research applied the residual selection method to a UAV. The feature point detection utilises multi‐scale and Gaussian model fitting techniques to guarantee true positives. The map is represented by Gaussian Mixture Models (GMM) with lower memory costs. The orthogonal laser scan device is composed and placed on a UAV for real‐time three‐dimensional localization, whose errors are at the centimeter level.