bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel sucrose-inducible expression system and its application for production of biomass-degrading enzymes in Aspergillus niger
Authors: Wang Lu , Xie Yijia , Chang Jingjing , Wang Juan , Liu Hong , Shi Mei , Zhong Yaohua 
Published: February 2023

Abstract
Background
Filamentous fungi are extensively exploited as important enzyme producers due to the superior secretory capability. However, the complexity of their secretomes greatly impairs the titer and purity of heterologous enzymes. Meanwhile, high-efficient evaluation and production of bulk enzymes, such as biomass-degrading enzymes, necessitate constructing powerful expression systems for bio-refinery applications.

Results
A novel sucrose-inducible expression system based on the host strain Aspergillus niger ATCC 20611 and the β-fructofuranosidase promoter (PfopA) was constructed. A. niger ATCC 20611 preferentially utilized sucrose for rapid growth and β-fructofuranosidase production. Its secretory background was relatively clean because β-fructofuranosidase, the key enzyme responsible for sucrose utilization, was essentially not secreted into the medium and the extracellular protease activity was low. Furthermore, the PfopA promoter showed a sucrose concentration-dependent induction pattern and was not subject to glucose repression. Moreover, the strength of PfopA was 7.68-fold higher than that of the commonly used glyceraldehyde-3-phosphate dehydrogenase promoter (PgpdA) with enhanced green fluorescence protein (EGFP) as a reporter. Thus, A. niger ATCC 20611 coupled with the PfopA promoter was used as an expression system to express a β-glucosidase gene (bgla) from A. niger C112, allowing the production of β-glucosidase at a titer of 17.84 U/mL. The crude β-glucosidase preparation could remarkably improve glucose yield in the saccharification of pretreated corncob residues when added to the cellulase mixture of Trichoderma reesei QM9414. The efficacy of this expression system was further demonstrated by co-expressing the T. reesei-derived chitinase Chi46 and β-N-acetylglucosaminidase Nag1 to obtain an efficient chitin-degrading enzyme cocktail, which could achieve the production of N-acetyl-D-glucosamine from colloidal chitin with a conversion ratio of 91.83%. Besides, the purity of the above-secreted biomass-degrading enzymes in the crude culture supernatant was over 86%.

Conclusions
This PfopA-driven expression system expands the genetic toolbox of A. niger and broadens the application field of the traditional fructo-oligosaccharides-producing strain A. niger ATCC 20611, advancing it to become a high-performing enzyme-producing cell factory. In particular, the sucrose-inducible expression system possessed the capacity to produce biomass-degrading enzymes at a high level and evade endogenous protein interference, providing a potential purification-free enzyme production platform for bio-refinery applications.
β-Glucan alleviates goal-directed behavioral deficits in mice infected with Toxoplasma gondii
Authors: Cui Zeyu , Gong Yuying , Luo Xiaotong , Zheng Niuyi , Tan Shimin , Liu Shuxi , Li Youwei , Wang Qingling , Sun Fenfen , Hu Minmin , Pan Wei , Yang Xiaoying 
Published: February 2023

Abstract
Background
Toxoplasma gondii (T. gondii) is a neuroinvasive parasite causing neuroinflammation, which in turn is associated with a higher risk for several psycho-behavioral disorders. There is an urgent need to identify drugs capable of improving cognitive deficits induced by T. gondii infection. β-Glucan, an active ingredient in mushrooms, could significantly enhance immunity. However, the effects of β-glucan against neuroinflammation and cognitive decline induced by T. gondii infection remain unknown. The present study aimed to investigate the neuroprotective effect of β-glucan on goal-directed behavior of mice chronically infected by T. gondii Wh6 strain.

Methods
A mice model of chronic T. gondii Wh6 infection was established by infecting mice by oral gavage with 10 cysts of T. gondii Wh6. Intraperitoneal injection of β-glucan was manipulated 2 weeks before T. gondii infection. Performance of the infected mice on the Y-maze test and temporal order memory (TOM) test was used to assess the goal-directed behavior. Golgi-Cox staining, transmission electron microscopy, immunofluorescence, real-time PCR and western blot assays were used to detect prefrontal cortex-associated pathological change and neuroinflammation.

Results
The administration of β-glucan significantly prevented T. gondii Wh6-induced goal-directed behavioral impairment as assessed behaviorally by the Y-maze test and TOM test. In the prefrontal cortex, β-glucan was able to counter T. gondii Wh6-induced degeneration of neurites, impairment of synaptic ultrastructure and decrease of pre- and postsynaptic protein levels. Also, β-glucan significantly prevented the hyperactivation of pro-inflammatory microglia and astrocytes, as well as the upregulation of proinflammatory cytokines caused by chronic T. gondii Wh6 infection.

Conclusions
This study revealed that β-glucan prevents goal-directed behavioral impairment induced by chronic T. gondii infection in mice. These findings suggest that β-glucan may be an effective drug candidate to prevent T. gondii-associated psycho-behavioral disorders including goal-directed behavioral injury.

Graphical Abstract
Echocardiographic assessment of Xenopus tropicalis heart regeneration
Authors: Lv Luocheng , Guo Weimin , Guan Wei , Chen Yilin , Huang Ruijin , Yuan Ziqiang , Pu Qin , Feng Shanshan , Zheng Xin , Li Yanmei , Xiao Luanjuan , Zhao Hui , Qi Xufeng , Cai Dongqing 
Published: February 2023

Abstract
Background
Recently, it was reported that the adult X. tropicalis heart can regenerate in a nearly scar-free manner after injury via apical resection. Thus, a cardiac regeneration model in adult X. tropicalis provides a powerful tool for recapitulating a perfect regeneration phenomenon, elucidating the underlying molecular mechanisms of cardiac regeneration in an adult heart, and developing an interventional strategy for the improvement in the regeneration of an adult heart, which may be more applicable in mammals than in species with a lower degree of evolution. However, a noninvasive and rapid real-time method that can observe and measure the long-term dynamic change in the regenerated heart in living organisms to monitor and assess the regeneration and repair status in this model has not yet been established.

Results
In the present study, the methodology of echocardiographic assessment to characterize the morphology, anatomic structure and cardiac function of injured X. tropicalis hearts established by apex resection was established. The findings of this study demonstrated for the first time that small animal echocardiographic analysis can be used to assess the regeneration of X. tropicalis damaged heart in a scar-free perfect regeneration or nonperfect regeneration with adhesion manner via recovery of morphology and cardiac function.

Conclusions
Small animal echocardiography is a reliable, noninvasive and rapid real-time method for observing and assessing the long-term dynamic changes in the regeneration of injured X. tropicalis hearts.
Traceability and quality assessment of Norway spruce (Picea abies (L.) H.Karst.) logs: the TreeTrace_spruce database
Authors: Longuetaud Fleur , Schraml Rudolf , Mothe Frédéric , Ravoajanahary Tojo , Decelle Rémi , Constant Thiéry , Ngo Phuc , Debled-Rennesson Isabelle , Entacher Karl , Petutschnigg Alexander , Brüchert Franka , Uhl Andreas 
Published: February 2023

Abstract
Key message
The TreeTrace_spruce database contains images and measurements of 100 Norway spruce (Picea abies (L.) H.Karst.) logs from Northeastern France, each about 4.5 m long. The image database includes RGB images of large and small ends of the logs and hyperspectral and computed tomography (CT) images of wood discs sampled at both log ends. The 100 logs were also fully X-ray scanned with a CT device for roundwoods and their top surface was scanned with a terrestrial LiDAR device. The measurements performed on discs include wood local density, growth ring widths and pith location. This database is complementary to another one (TreeTrace_Douglas) resulting from the same ANR project TreeTrace, but if the objectives are similar, the protocols and conditions of acquisition are not the same for these two databases. TreeTrace_spruce dataset is available at https://doi.org/10.57745/WKLTJI and associated metadata are available at https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/cffee2f1-18e1-4b53-9f5b-6cc4c66f1cb8.
Mulched and soil-incorporated sugarcane greenchop residue and compost: effects on selected soil components, sugarcane nutrients, Mexican rice borer injury, and yield
Authors: Showler Allan T. 
Published: February 2023

AbstractBurning sugarcane,Saccharumspp., fields to remove leaves before harvest is a routine practice in many sugarcane production areas, including the United States. The method is environmentally deleterious and has been discontinued in some parts of the world. Alternatively, excised preharvest leaf residue, or greenchop, is used as mulch. This field study examined the effects of greenchop, applied in several ways to sugarcane soil, on soil fertility, selected sugarcane plant physiochemicals, injury inflicted by the Mexican rice borer,Eoreuma loftini(Dyar) (Lepidoptera: Crambidae), and sugarcane yield parameters, compared to plots augmented by soil-incorporated compost and a nontreated control. The compost amended soil had elevated concentrations of many nutrients, and in sugarcane leaves, heightened concentrations of certain sugars and free amino acids. None of the greenchop treatments affected soil and sugarcane leaf nutrients. During the first year of the study,E. loftiniinjury to sugarcane stalks during the first season, however, was generally greater in the greenchop and compost treatments than in the control. In the second growing season, the compost treatment was the only treatment associated with heightenedE. loftiniinfestations. Relationships between soils augmented with organic amendments andE. loftiniinjury to sugarcane are discussed in terms of mediation through physiochemical changes induced by the amendments.
Revealing the complexity of vampire bat rabies “spillover transmission”
Authors: Escobar Luis E. , Velasco-Villa Andres , Satheshkumar Panayampalli S. , Nakazawa Yoshinori , Van de Vuurst Paige 
Published: February 2023

Abstract
Background
The term virus ‘spillover’ embodies a highly complex phenomenon and is often used to refer to viral transmission from a primary reservoir host to a new, naïve yet susceptible and permissive host species. Spillover transmission can result in a virus becoming pathogenic, causing disease and death to the new host if successful infection and transmission takes place.

Main text
The scientific literature across diverse disciplines has used the terms virus spillover, spillover transmission, cross-species transmission, and host shift almost indistinctly to imply the complex process of establishment of a virus from an original host (source/donor) to a naïve host (recipient), which have close or distant taxonomic or evolutionary ties. Spillover transmission may result in unsuccessful onward transmission, if the virus dies off before propagation. Alternatively, successful viral establishment in the new host can occur if subsequent secondary transmission among individuals of the same novel species and among other sympatric susceptible species occurred. As such, virus spillover transmission is a common yet highly complex phenomenon that encompasses multiple subtle stages that can be deconstructed to be studied separately to better understand the drivers of disease emergence. Rabies virus (RABV) is a well-documented viral pathogen which still inflicts heavy impact on humans, companion animals, wildlife, and livestock throughout Latin America due substantial spatial temporal and ecological—natural and expansional—overlap with several virus reservoir hosts. Thereby, the rabies disease system represents a robust avenue through which the drivers and uncertainties surrounding spillover transmission can be unravel at its different subtle stages to better understand how they may be affected by coarse, medium, and fine scale variables.

Conclusions
The continued study of viral spillover transmission necessitates the elucidation of its complexities to better assess the cross-scale impacts of ecological forces linked to the propensity of spillover success. Improving capacities to reconstruct and predict spillover transmission would prevent public health impacts on those most at risk populations across the globe.

Graphical Abstract:
Receiver mount design, transmitter depth, and wind speed affect detection probability of acoustic telemetry transmitters in a Missouri River tributary
Authors: Carlson Tanner L. , LaBrie Lindsey A. P. , Wesner Jeff S. , Chipps Steven R. , Coulter Alison A. , Schall Benjamin J. 
Published: February 2023

Abstract
Background
One of the most important considerations for acoustic telemetry study designs is detection probability between the transmitter and the receiver. Variation in environmental (i.e., wind and flow) and abiotic (i.e., bathymetry) conditions among aquatic systems can lead to differences in detection probability temporally or between systems. In this study we evaluate the effect of distance, receiver mount design, transmitter depth, and wind speed on detection probabilities of two models of acoustic transmitters in a mid-sized river. InnovaSea V16-6H (hereafter V16) and V13-1L (hereafter V13) tags were deployed in the James River, SD at 0.36 m (deep) and 2.29 m (V16 tag) or 1.98 m (V13 tag; shallow) above the benthic surface downstream of InnovaSea VR2W stationary receivers at distances of 100, 200, or 300 m. We used two receiver mount designs that included a fixed position within a PVC pipe on the downstream side of a bridge piling or a metal frame deployed in the middle of the river channel. Tags were deployed for 72 h at each location, and hourly detections were summarized. We evaluated downstream distance, receiver mount design, tag depth, and wind effects on tag detection using Bayesian logistic regression.

Results
Detection probability decreased as distance increased for all combinations of tag types and mount designs and varied from nearly 100% at 100 m to less than 10% at 300 m. The V16 transmitter had greater detection probability by the receiver mounted in the pipe than in the midriver frame. For both mounts, the deep V16 transmitter had greater detection probability than the V16 shallow transmitter. Detection probability of the V13 transmitter was similar between receiver mounts or transmitter depths. Wind speed had a negative impact on detection probabilities of both transmitter types and depths, except the deep V16 transmitter.

Conclusions
Deploying acoustic receivers in PVC pipes rather than midriver frames provided greater downstream detection probabilities for V16 transmitters under conditions evaluated in this study. In addition, V16 transmitters had greater detection probabilities when positioned deep within the water column rather than near the surface. We also demonstrated that wind speed can have a negative impact on detection probabilities.
Assessment and management of disorders of gut–brain interaction in patients with eating disorders
Authors: Atkins Micaela , Burton Murray Helen , Staller Kyle 
Published: February 2023

AbstractDisorders of gut–brain interaction (DBGI), also known as functional gastrointestinal disorders, are common in individuals with eating disorders, and may precede or perpetuate disordered eating. Understanding the pathophysiology of common gastrointestinal symptoms in DGBI can be important for the care of many patients with eating disorders. In this review, we summarize the literature to date on the complex relationship between DBGI and eating disorders and provide guidance on the assessment and management of the most common symptoms of DBGI by anatomic region: esophageal symptoms (globus and functional dysphagia), gastroduodenal symptoms (functional dyspepsia and nausea), and bowel symptoms (abdominal pain, bloating and constipation).
CNS tumor with EP300::BCOR fusion: discussing its prevalence in adult population
Authors: Tauziède-Espariat Arnault , Uro-Coste Emmanuelle , Sievers Philipp , Nicaise Yvan , Mariet Cassandra , Siegfried Aurore , Pierron Gaëlle , Guillemot Delphine , Benzakoun Joseph , Pallud Johan , Roques Margaux , Bonneville Fabrice , Larrieu-Ciron Delphine , Chaynes Patrick , Saffroy Raphaël , Hamelin Jocelyne , Hasty Lauren , Métais Alice , Chrétien Fabrice , Kool Marcel , Gojo Johannes , Varlet Pascale ,   RENOCLIP-LOC
Published: February 2023

AbstractThe Central Nervous System (CNS) tumor with BCOR internal tandem duplication (ITD) has recently been added as a novel embryonal histomolecular tumor type to the 2021 World Health Organization (WHO) Classification of CNS Tumors. In addition, other CNS tumors harboring a BCOR/BCORL1 fusion, which are defined by a distinct DNA-methylation profile, have been recently identified in the literature but clinical, radiological and histopathological data remain scarce. Herein, we present two adult cases of CNS tumors with EP300::BCOR fusion. These two cases presented radiological, histopathological, and immunohistochemical homologies with CNS tumors having BCOR ITD in children. To compare these tumors with different BCOR alterations, we performed a literature review with a meta-analysis. CNS tumors with EP300::BCOR fusion seem to be distinct from their BCOR ITD counterparts in terms of age, location, progression-free survival, tumor growth pattern, and immunopositivity for the BCOR protein. CNS tumors from the EP300::BCOR fusion methylation class in adults may be added to the future WHO classification.
Analysis of anticancer potential of Kingiodendron pinnatum (DC.) Harms
Authors: Pareeth Chennattu M. , Meera Nair , Silpa Prabha , Thara Kannoor M. , Raghavamenon Achuthan C. , Babu Thekkekara D. 
Published: February 2023

Abstract
Background
The plant Kingiodendron pinnatum (DC.) Harms, belonging to the family Fabaceae is endemic to the Western Ghats of India and is commonly used for various ailments, especially by the tribes. K. pinnatum is occasionally used as a substitute for Saraca asoca in Asokarishta, a well-known uterine tonic in Ayurveda. Recent studies revealed a pharmacological similarity between the plants. S. asoca is reported to have anti-cancer properties, but there are no reports on K. pinnatum except for antioxidant and antimicrobial activities. Therefore, the study is aimed to investigate the anticancer potential of the plant.

Methods
Cytotoxicity of methanolic bark extract of the plant was analysed on different cancer cell lines by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Dalton's lymphoma ascites (DLA) cell-induced solid and Ehrlich ascites carcinoma (EAC) cell-induced ascites tumour models in mice were used to study the antitumor potential. Phytochemical screening of the extract was also performed.

Results
The extract was found cytotoxic to DLA, EAC, HCT15, MDA-MB-231, T47D and PC3 with inhibitory concentration (IC50) values of 50.09, 74.74, 67.02, 119.22, 149.04 and 194.5 μg/mL, respectively. In the solid tumour model, a significant (P < 0.001) reduction in tumour weight of 0.7 ± 0.15 g was observed in 500 mg/kg b.wt. extract treated group compared to the control group (3.6 ± 0.24 g) by oral administration for 30 days. In the ascites tumour model, a high survival rate of 28.2 ± 8.72 days (P < 0.01) was found by the extract treatment compared to the control animals. Phytochemicals like alkaloids, flavonoids, phenols, phytosterols, saponins, tannins, steroids and terpenoids were detected in the extract.

Conclusion
Results obtained by the cytotoxic and anti-tumour studies revealed the anticancer potential of K. pinnatum. The plant exhibits more cytotoxicity towards cancer cell lines of the reproductive system such as the breast and prostate.
Authenticating GNSS civilian signals: a survey
Authors: Yuan Muzi , Tang Xiaomei , Ou Gang 
Published: February 2023

AbstractCivilian services of Global Navigation Satellite System are threatened by spoofing attacks since it is hard to determine the authenticity of a navigation signal with a detailed structure open to the public. Signal authentication effectively protects the security of the signal by attaching unforgeable information to one or several elements of the signal. Receivers can verify the authenticity of the signal by extracting and validating this information. Developing good signal authentication schemes requires understanding possible spoofing modes, signal element specialty, and performance evaluation methods. This paper is an overview of navigation signal authentication, where the theories and reported approaches are described in detail. A design/performance matrix that demonstrates the advantages and defects of the signal element and its authentication design is summarized. Recommendations are proposed to improve the robustness, security, efficiency, and implementation hardness for future designs of navigation signal authentication.
Addressing key issues in HIV self-test program implementation for Black and Latino sexual minority men in the Southern United States: a multiphase study protocol
Authors: Guigayoma John , Becker Sara J. , Ong Jason J. , Kanamori Mariano , Hickson DeMarc , Ward Lori M. , Biello Katie B. , Wray Tyler 
Published: February 2023

Abstract
Background
Black and Latino sexual minority men in the Southern United States have the highest HIV infection rates in the country. Increased HIV testing can help decrease onward HIV transmission through detecting previously undiagnosed infections. HIV self-testing is an evidence-based strategy to increase HIV testing among sexual minority men, but the implementation of this intervention in the Southern United States is limited. One implementation barrier is the lack of knowledge of Black and Latino sexual minority men’s preferences for various HIV self-testing program characteristics and their willingness to pay for these preferences. In addition, little is known about facilitators and barriers to initiating HIV self-testing programs from the perspectives of HIV prevention implementation decision-makers in this region.

Methods
We will conduct an online discrete choice experiment among Black and Latino sexual minority men in the Southern United States (n = 300) to estimate this population’s preferences for the following HIV self-testing program characteristics: delivery strategy (home delivery, peer delivery, clinic pickup); delivery speed (same day, next day, 3 days, and 5 days); support (instructions only, during test, and 1 week after delivery); and price ($0, $20, $40, $50, $60). We will also use this choice data to generate willingness-to-pay estimates for each program characteristic. Guided by the Consolidated Framework for Implementation Research, we will then conduct semi-structured interviews (n = 30) with HIV prevention program decision-makers at various health organizations serving Black and Latino sexual minority men in the region to further understand facilitators and barriers to implementation of the most preferred HIV self-testing program design.

Discussion
By gaining perspectives on HIV self-testing implementation from patients and providers, this project will build a roadmap for the initiation of HIV self-testing programs to decrease HIV incidence among one of the most disproportionately impacted populations in the USA.