bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification of late embryogenesis abundant protein family and their key regulatory network in Pinus tabuliformis cold acclimation
Authors: Zhou Chengcheng , Niu Shihui , El-Kassaby Yousry A , Li Wei 
Published: August 2023

Abstract
Cold acclimation is a crucial biological process that enables conifers to overwinter safely. The late embryogenesis abundant (LEA) protein family plays a pivotal role in enhancing freezing tolerance during this process. Despite its importance, the identification, molecular functions and regulatory networks of the LEA protein family have not been extensively studied in conifers or gymnosperms. Pinus tabuliformis, a conifer with high ecological and economic values and with high-quality genome sequence, is an ideal candidate for such studies. Here, a total of 104 LEA genes were identified from P. tabuliformis, and we renamed them according to their subfamily group: PtLEA1–PtLEA92 (group LEA1–LEA6), PtSMP1–PtSMP6 (group seed maturation protein) and PtDHN1–PtDHN6 (group Dehydrin). While the sequence structure of P. tabuliformis LEA genes are conserved, their physicochemical properties exhibit unique characteristics within different subfamily groupings. Notably, the abundance of low-temperature responsive elements in PtLEA genes was observed. Using annual rhythm and temperature gradient transcriptome data, PtLEA22 was identified as a key gene that responds to low-temperature induction while conforming to the annual cycle of cold acclimation. Overexpression of PtLEA22 enhanced Arabidopsis freezing tolerance. Furthermore, several transcription factors potentially co-expressed with PtLEA22 were validated using yeast one-hybrid and dual-luciferase assays, revealing that PtDREB1 could directly bind PtLEA22 promoter to positively regulate its expression. These findings reveal the genome-wide characterization of P. tabuliformis LEA genes and their importance in the cold acclimation, while providing a theoretical basis for studying the molecular mechanisms of cold acclimation in conifers.
Prevalence and associated factors of stunting and thinness among adolescent Sudanese schoolchildren: a cross-sectional study
Authors: Bilal Jalal A , Osman Almarwa A , Al-Nafeesah Abdullah , AlEed Ashwaq , Adam Ishag 
Published: August 2023

ABSTRACT

Background
Malnutrition among adolescents is a considerable health problem worldwide. There are scarce data on undernutrition among adolescents in Sudan.

Methods
A cross‑sectional study was conducted to investigate the prevalence and associated factors of stunting and thinness among adolescent schoolchildren in northern Sudan. The questionnaires were used to collect information on sociodemographics. Weights and heights were measured and the height-for-age and body mass index-for-age cut-offs recommended by the WHO were applied, followed by logistic regression analysis.

Results
Of 384 enrolled adolescents, 202 (52.6%) were females and 182 (47.4%) were males. The median (IQR) age of these adolescents was 15.1 (14.0‒16.3) y. Seventy-six (19.8%) and 52 (15.4%) adolescents had stunting and thinness, respectively, and 15 (3.9%) had both stunting and thinness. Multivariate analysis showed that increased age (adjusted OR [AOR]=1.30, 95% CI 1.08 to 1.57) and male gender (AOR=5.82, 95% CI 3.11 to 10.91) were associated with stunting. Male gender (AOR=2.08, 95% CI 1.14 to 3.82) and smoking/tobacco snuff (AOR=2.61, 95% CI 1.07 to 6.36) were associated with thinness.

Conclusions
The findings of the current study are that both stunting and thinness are important health problems, especially among boys, older participants and smokers.
The largest Palaeozoic whip scorpion and the smallest (Arachnida: Uropygi: Thelyphonida); a new species and a new ichnospecies from the Carboniferous of New England, USA
Authors: Knecht Richard J , Benner Jacob S , Dunlop Jason A , Renczkowski Mark D 
Published: August 2023

Abstract
Palaeozoic fossils of whip scorpions (Arachnida: Uropygi: Thelyphonida) are extremely rare, with only seven species of this age previously described. A new species of fossil whip scorpion, as well as the first ichnospecies assignable to this group, are described here from the Carboniferous Narragansett Basin of Massachusetts, USA. A body fossil from the Rhode Island Formation (Moscovian) is referred to as Parilisthelyphonus bryantae gen. nov., sp. nov.. At more than 34 mm long it represents both the largest known Palaeozoic whip scorpion and the first fossil arachnid found in the Rhode Island Formation in ~130 years of scrutiny. The whip scorpion trace fossil, comprising a full-body impression and associated tracks, is described from the subjacent Wamsutta Formation (Late Bashkirian) of Massachusetts, USA as Inmontibusichnus charleshenryturneriigen. nov., isp. nov.. With an estimated body length of less than 10 mm, the producer would be the smallest known Palaeozoic thelyphonid. These discoveries within the Narragansett Basin represent only the second site in the western hemisphere, in what was western Laurasia, to yield Palaeozoic whip scorpions. The Narragansett Basin is of significant Pangaean biogeographical importance among whip scorpion sites, being located between the westernmost Mazon Creek Lagerstätte of Illinois and the eastern assemblage of localities in Europe, and serves as an important new fossil calibration point for phylogenetic studies of this arachnid group.
Electrical Impedance Tomography Identifies Evolution of Regional Perfusion in a Porcine Model of Acute Respiratory Distress Syndrome
Authors: Martin Kevin T. , Xin Yi , Gaulton Timothy G. , Victor Marcus , Santiago Roberta R. , Kim Taehwan , Morais Caio C. A. , Kazimi Aubrey A. , Connell Marc , Gerard Sarah E. , Herrmann Jacob , Mueller Ariel L. , Lenart Austin , Shen Jiacheng , Khan Sherbano S. , Petrov Mihail , Reutlinger Kristan , Rozenberg Karina , Amato Marcelo , Berra Lorenzo , Cereda Maurizio 
Published: August 2023

Background
Bedside electrical impedance tomography could be useful to visualize evolving pulmonary perfusion distributions when acute respiratory distress syndrome worsens or in response to ventilatory and positional therapies. In experimental acute respiratory distress syndrome, this study evaluated the agreement of electrical impedance tomography and dynamic contrast–enhanced computed tomography perfusion distributions at two injury time points and in response to increased positive end-expiratory pressure (PEEP) and prone position.

Methods
Eleven mechanically ventilated (VT 8 ml · kg−1) Yorkshire pigs (five male, six female) received bronchial hydrochloric acid (3.5 ml · kg−1) to invoke lung injury. Electrical impedance tomography and computed tomography perfusion images were obtained at 2 h (early injury) and 24 h (late injury) after injury in supine position with PEEP 5 and 10 cm H2O. In eight animals, electrical impedance tomography and computed tomography perfusion imaging were also conducted in the prone position. Electrical impedance tomography perfusion (QEIT) and computed tomography perfusion (QCT) values (as percentages of image total) were compared in eight vertical regions across injury stages, levels of PEEP, and body positions using mixed-effects linear regression. The primary outcome was agreement between QEIT and QCT, defined using limits of agreement and Pearson correlation coefficient.

Results
Pao
2/Fio
2 decreased over the course of the experiment (healthy to early injury, −253 [95% CI, −317 to −189]; early to late injury, −88 [95% CI, −151 to −24]). The limits of agreement between QEIT and QCT were −4.66% and 4.73% for the middle 50% quantile of average regional perfusion, and the correlation coefficient was 0.88 (95% CI, 0.86 to 0.90]; P < 0.001). Electrical impedance tomography and computed tomography showed similar perfusion redistributions over injury stages and in response to increased PEEP. QEIT redistributions after positional therapy underestimated QCT in ventral regions and overestimated QCT in dorsal regions.

Conclusions
Electrical impedance tomography closely approximated computed tomography perfusion measures in experimental acute respiratory distress syndrome, in the supine position, over injury progression and with increased PEEP. Further validation is needed to determine the accuracy of electrical impedance tomography in measuring perfusion redistributions after positional changes.

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Contribution of cardiovascular disease to the burden of non-communicable diseases in Africa: an analysis of data from Global Burden of Disease database, 1990–2019
Authors: Maimaitiming Mailikezhati , Kakunze Adelard , Feng Yikai , Wang Minmin , Li Na , Shi Junyi , Huang Kepei , Jin Yinzi , Zheng Zhi-Jie 
Published: August 2023

Background and purpose:
Disease burden of non-communicable diseases (NCDs) has been increasing in low- and middle-income countries, especially in African countries. Cardiovascular disease (CVD) is a common NCDs and the leading cause of death in Africa. Yet, evidence on the contribution of CVD to the total NCDs burden is scarce. This study aimed to investigate the contribution of CVD to the total disease burden caused by NCDs in Africa between 1990 and 2019.

Methods:
Data on NCDs, including CVD, were extracted from the Global Burden of Disease (GBD) database. Disease burden was measured by disability-adjusted life years (DALYs) and deaths.

Results:
From 1990 to 2019, the share of CVD in the total NCDs burden increased and remained as the first contributor. In 2019, NCDs caused 204 million DALYs and over 2.18 million deaths in the Africa Union, among which 49.1% of the total DALYs and 66.0% of deaths were caused by the traditional five NCDs, including CVD that accounted for the largest burden of NCDs (20.5% of DALYs and 35.2% of deaths). The proportion of CVD to the total NCDs burden was most considerable, reaching up to 32.4% and 52.4% in Northern Africa for DALYs and deaths, respectively. CVD were important contributors to NCDs burden in Africa, including ischemic heart disease (1334 DALYs per 100,000 and 27.79 deaths per 100,000) and intracerebral hemorrhage (632 DALYs per 100,000 and 13.53 deaths per 100,000) that were the top two causes of NCDs in 2019.

Conclusions:
CVD has been the biggest contributor to NCDs burden in the Africa Union over the thirty years. Given the increase in the share of NCDs burden attributable to CVD, it demands a close attention to implementing cost-effective policies and interventions for CVD to achieve an improvement in population health and reduction of health loss from NCDs.
The Role of Eye Banking in Implementation of the Integrated People-Centered Eye Care (IPEC) Model
Authors: Schwarz Kaitlin , Bashshur Ramona 
Published: August 2023

Abstract:
Vision loss and blindness are global public health concerns. Measures must be taken to address the magnitude and causes. The integrated people-centered model of eye care (IPEC) has been advanced by the World Health Organization and the International Agency to Prevent Blindness to address these challenges and facilitate the delivery of eye care. The US eye banking community occupies a distinct sector within the spectrum of vision services and global public health efforts. Alignment with the IPEC model and these global priorities is a logical development for the cornea and eye banking sector, and addressing gaps in the evidence base for corneal disease will serve to strengthen these efforts.
Prevalence of International Medical Graduates in Integrated Plastic Surgery Programs
Authors: Porras Fimbres Denisse Cristina , Zeng Steven , Phillips Brett Thomas 
Published: August 2023

Background:
International medical graduates (IMGs) are physicians who did not attend medical school in the USA or Canada. IMGs comprise nearly one-quarter of the physician workforce and play a vital role in health care. Here, we aimed to identify the prevalence of IMGs in integrated programs and evaluate factors that influence their success in the residency match.

Methods:
The annual match reports from 2010 to 2020 were retrieved and summarized. Electronic surveys for program directors and program coordinators were distributed to US integrated plastic surgery programs. Each program’s website was appraised for information regarding the eligibility of IMGs. Websites were also used to identify the number of IMG residents.

Results:
The number of applicants who matched into integrated programs ranged from 69 to 180 per year, of which US applicants comprised 61–165. US IMGs filled one to three positions per year, whereas non-US IMGs filled two to seven. Although 48% of programs have matched non-citizen IMGs and 79% have not encountered difficulties during the visa process, 67% of coordinators reported that the onboarding process is more challenging for IMGs. There are no IMGs in 52% of programs, and most institutions offer information on their website regarding visa sponsorship.

Conclusion:
IMGs make up less than 10% of filled positions per cycle. Although most programs accept IMGs, a small number matriculate. This may be explained by the competitiveness of integrated programs and the volume of IMG applications. Further research is needed to identify contributing factors of low IMG representation in plastic surgery programs.
Analysis on the Difference between the Practical Brassiere Size and Real Breast Volume
Authors: Lee Seok Joon , Han Woo Yeon , Eom Jin Sup , Kim Eun Key , Han Hyun Ho 
Published: August 2023

Background:
Brassiere cup size is defined as the difference in chest circumference between the inframammary fold and the fullest part of the breast. However, a large number of women are not aware of the correct definition and are prone to wearing incorrectly-sized brassieres. In this report, the authors compared the cup size of worn brassieres and the actual measurement.

Methods:
This study was a retrospective review of patients who had undergone breast reconstruction operation between May 2020 and June 2021. All patients who visited the plastic surgery clinic for breast reconstruction were inquired about their cup size, and their breast circumferences were measured. The patient demographic information, ptosis grade, mastectomy specimen weight, measured breast circumference, and known cup size were analyzed.

Results:
Overall, 163 women were included. Notably, 92 of 163 patients (56.4%) were wearing a correctly-sized brassiere. Patients were more likely to wear a correctly-sized brassiere as the cup size became smaller. Moreover, patients with A-cup breasts tended to wear larger brassieres, whereas patients with B and C-cup breasts tended to wear smaller brassieres than their actual breast cup size.

Conclusions:
Approximately one in two women do not know their correct brassiere cup size. Women tend to wear a brassiere of the wrong size as their cup size becomes larger. Therefore, it is important for surgeons to be aware of their patient’s brassiere wearing habit and their perception when a surgery, such as augmentation or reconstruction, is planned.
Recognition and Respect: Contextualizing the History and Contributions of Black American Plastic Surgeons
Authors: Glahn Joshua Zev , Hooper Rachel C. , Butler Paris D. 
Published: August 2023

Summary:
Recently, there has been heightened interest in the history of Black American plastic surgeons and their contributions to the field of plastic and reconstructive surgery (PRS). Despite the increased awareness and attention toward the lack of racial and ethnic diversity of the PRS workforce, the history of how PRS became one of the most ethnically segregated surgical specialties remains unexplored. Here, we outline the various political and cultural factors that contributed to the exclusion of Black practitioners from American PRS professional societies. This work contextualizes the rise of American PRS within the Jim Crow era and highlights the cultural significance of reconstructive procedures performed in the treatment of disfigured soldiers. Through this lens, we identify circumstances where Black surgeons were systematically denied opportunities to participate in the emerging specialty. Despite these barriers, we demonstrate how Black physicians established informal networks for professional advancement and shed light on several previously unrecognized contributions to PRS from Black surgeons. In addition, we explore how the inclusion of Black voices in PRS sparked a paradigm shift in the treatment of non-White patients that expanded the cosmetic marketplace in ways that remain significant today. Finally, we situate the ongoing disparities in Black representation in PRS within a broader historical narrative and illustrate how the stories we tell about our past continue to shape the future of our field.
Estimation of Mastectomy Volume Using Preoperative Mastectomy Simulation Images Acquired by the Vectra H2 System
Authors: Kim Jiwoo , Matsumine Hajime , Niimi Yosuke , Osada Atsuyoshi , Sakurai Hiroyuki 
Published: August 2023

Background:
Preoperative prediction of breast volume is very important in planning breast reconstruction. In this study, we assessed the usefulness of a novel method for preoperative estimation of mastectomy volume by comparing the weight of actual mastectomy specimens with the values predicted by the developed method using the Vectra H2.

Methods:
All patients underwent skin-sparing mastectomy and immediate autologous breast reconstruction. Preoperatively, the patient’s breast was scanned using the Vectra H2 and a postmastectomy simulation image was constructed on a personal computer. The estimated mastectomy volume was calculated by comparing the preoperative and postmastectomy three-dimensional simulation images. Correlation coefficients with the estimated mastectomy volume were calculated for the actual mastectomy weight and the transplanted flap weight.

Results:
Forty-five breasts of 42 patients were prospectively analyzed. The correlations with the estimated mastectomy volume were r = 0.95 (P < 0.0001) for actual mastectomy weight and r = 0.84 (P < 0.0001) for transplanted free-flap weight. The mastectomy weight estimation formula obtained by linear regression analysis using the estimated mastectomy volume was 0.98 × estimated mastectomy volume + 5.4 (coefficient of determination R2 = 0.90, P < 0.0001). The root-mean-square error for the mastectomy weight estimation formula was 38 g.

Conclusions:
We used the Vectra H2 system to predict mastectomy volume. The predictions provided by this method were highly accurate. Three-dimensional imaging is a noncontact, noninvasive measurement method that is both accurate and simple to perform. Use of this effective tool for volume prediction is expected to increase in the future.
Nerve Transfers Using a Dedicated Microsurgical Robotic System
Authors: Schäfer Benedikt , Bahm Jörg , Beier Justus P. 
Published: August 2023

Summary:
Microsurgical demands in peripheral nerve surgery are increasing. Because of the development of multiple simultaneous selective nerve transfers, the transposition of very small nerves and even single fascicles has evolved. Coaptation of these increasingly smaller structures require high skills in microsurgical techniques. In addition, the surgical situs often has very limited access and is difficult to reach with conventional microsurgical options. Robot technology, the Symani Surgical System (Medical Microinstruments, S.p.A, Calci, Pisa, Italy), was used for epineural coaptation of three donor nerves (intercostal nerves 4–6) to the long thoracic nerve and the thoracodorsal nerve as recipient nerves in a patient with brachial plexus palsy. The coaptations could be carried out successfully with the microsurgical robot technology. In combination with a high-magnification (up to 26×) 3D-exoscope, the epineural sutures could be placed very precisely and accurately. Using this new microsurgical robotic system, successful coaptation of very small nerve structures is possible. This opens possibilities for the microsurgeon to carry out even finer, more targeted and more complex nerve transfers, including procedures in anatomical regions that are difficult to reach.
Knee-saving Amputation by Use of a Bipedicled Four-zone Deep Inferior Epigastric Artery Perforator Flap
Authors: Saritas Sinem , Jul Kiil Birgitte , Sværdborg Mille 
Published: August 2023

Summary:
Degloving traumatic lower extremity injuries can lead to an above-knee amputation with decreased functional capacity compared with below-knee amputation. The unique properties of the deep inferior epigastric artery perforator flap providing a substantial amount of skin and subcutaneous tissue combined with reliability and low donor-site morbidity makes the flap ideal for coverage of below-knee amputation stumps when soft tissue is required. A bipedicled four-zone deep inferior epigastric artery perforator flap with a skin area of 13 × 33 cm was used as coverage of a degloved lower leg amputation stump, in a 27-year-old woman with a left-sided Gustillo type 3B comminute diaphyseal tibial fractur and major degloving injury. Hereby, above-knee amputation was avoided. Ten months postoperatively the patient had achieved full prosthetic function.
Enhanced Dosimetric Accuracy Using Quality Factor Compensation Method for In Vivo Electron Paramagnetic Resonance Tooth Dosimetry
Authors: Choi Kwon , Koo Chang Uk , Oh Jeonghun , Kim Jiwon , Park Jong In , Kim Sung Hwan , Lee Jong Hoon , Kang Dae Gyu , Ye Sung-Joon 
Published: August 2023

Abstract
We aim to develop a dose assessment method compensating for quality factors (Q factor) observed during in vivo EPR tooth dosimetry. A pseudo-in-vivo phantom made of tissue-equivalent material was equipped with one each of four extracted human central incisors. A range of Q factors was measured at tooth-depths of −2, 0, and 2 mm in the pseudo-in-vivo phantom. In addition, in vivo Q factors were measured from nine human volunteers. For the dose-response data, the above four sample teeth were irradiated at 0, 1, 2, 5, and 10 Gy, and the radiation-induced signals were measured at the same tooth-depths using an in vivo EPR tooth dosimetry system. To validate the method, the signals of two post-radiotherapy patients and three unirradiated volunteers were measured using the same system. The interquartile range of the Q factors measured in the pseudo-in-vivo phantom covered that observed from the human volunteers, which implied that the phantom represented the Q factor distribution of in vivo conditions. The dosimetric sensitivities and background signals were decreased as increasing the tooth-depth in the phantom due to the decrease in Q factors. By compensating for Q factors, the diverged dose-response data due to various Q factors were converged to improve the dosimetric accuracy in terms of the standard error of inverse prediction (SEIP). The Q factors of patient 1 and patient 2 were 98 and 64, respectively, while the three volunteers were 100, 92, and 99. The assessed doses of patient 1 and patient 2 were 2.73 and 12.53 Gy, respectively, while expecting 4.43 and 13.29 Gy, respectively. The assessed doses of the unirradiated volunteers were 0.53, 0.50, and − 0.22 Gy. We demonstrated that the suggested Q factor compensation could mitigate the uncertainty induced by the variation of Q factors.