bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forensic cases of starved dogs: Novel histologic-morphometric and immunohistochemical tools for atrophy assessment
Authors: Vázquez-Fernández Esther , Rebollada-Merino Agustín , Rodríguez-Bertos Antonio 
Published: September 2023

Abstract
Starvation, the involuntary loss of body mass, is one of the main causes of dog death related to animal abuse. Nevertheless, the mechanisms beyond tissue atrophy are not well understood. Dog forensic cases with a final diagnosis of starvation (n = 13), referred to [to be included after acceptance] between 2014 and 2021, were included in our study. These dogs had a body condition score (BCS) of 1 (38.4%) or 2 (61.6%) over 9 according to the World Small Animal Veterinary Association (WSAVA). Five dogs (BCS 5/9) that died due to other forensic causes were used as controls. The mechanisms underlying atrophy were studied by means of a histologic-morphometric and an immunohistochemical study (Ki-67 and caspase-3) of the spleen, liver, testis, stomach, and duodenum. Gross and histologic findings suggested tissue atrophy in all our starved dogs. Histologic-morphometric study confirmed atrophy in liver, spleen, testis, and duodenum; and increased gastric mucosal thickness in starved dogs. Ki-67 immunohistochemistry displayed fewer dividing cells in spleen, testis, stomach, and duodenum of starved dogs respect to controls. Caspase-3 immunostaining revealed an increased percentage of apoptotic cells in the spleen and testis of the starved group. These investigations contribute to a better understanding of the molecular mechanisms of starvation in dog abuse cases. To conclude, the combination of gross, histologic, histologic-morphometric, and immunohistochemical methods should be considered for the diagnosis and confirmation of tissue atrophy in forensic cases of animal abuse.
SSR analysis on the genetic diversity of Deinanthe caerulea Stapf., a rare and endemic plant in China
Authors: Xu Jie , xiao pengnan 
Published: September 2023

Abstract
Deinanthe caerulea Stapf. （D.caerulea） is a rare perennial herb endemic to China, with high medicinal and ornamental value. It is classified as VU endangered in the Chinese Species Red List (CSRL). Research on it has been extremely limited so far. In this paper, D.caerulea seeds were collected for morphological observation, vitality test, and germination experiments, and the results were as follows: the average fruit yield of D.caerulea seeds reached 1503.99 ± 483.64 per fruit, and there was a 30.26% abortion rate. TTC（2,3,5-Triphenyltetrazolium chloride） staining experiments showed that D.caerulea seeds had an activity rate of 86.33%. Light at 3000 lx and temperature at 25°C/15°C were the optimum conditions for seed germination. The results of seed germination experiments in the artificial incubator showed that the seed germination rate could reach 68.89% under the optimum conditions, but the suitable temperature range for seed germination was extremely narrow, which required a clever coupling between the external environment and the biological properties of seeds to ensure smooth seed germination, which was the main reason for the scarcity of seedling regeneration in the field. These factors, together with insufficient light in the forest understory to limit seed germination, are intrinsic to the difficulty of natural regeneration of this species.
Early peri-implant bone healing on laser-modified surfaces with and without hydroxyapatite coating: an in vivo study
Authors: Santos Ana Flávia Piquera , Silva Rodrigo Capalbo da , Hadad Henrique , de Jesus Laís Kawamata , Cervantes Lara Cristina Cunha , Nímia Heloisa Helena , Oliveira Sandra Helena Penha , Guastaldi Antonio Carlos , Queiroz Thallita Pereira , Poli Pier Paolo , Okamoto Roberta , Gruber Reinhard , Souza Francisley Ávila 
Published: September 2023

Abstract

Objective
The aim of this study was to assess the biological behavior of bone tissue on the machined surface (MS) and the modifications by laser beam (LS), and by laser beam incorporated with hydroxyapatite (HA) by biomimetic method without heat treatment (LHS).
Materials and methods
Scanning electron microscopy coupled with energy dispersive X-ray spectrometry (SEM/EDX) was performed before and after installation in the rabbit tibiae. 20 Albinus rabbits received 40 implants of 3.75x10mm in the right and left tibias, with one implant on each surface in each tibia. In the animals belonging to the 4-week euthanasia period, intramuscular application of the fluorochromes calcein and alizarin was performed. The tibias with the implants were submitted to analysis by confocal laser microscopy (mineral apposition rate – MAR) and histometric analysis by bone contact implant (%BIC) and newly formed bone area (%NBA).
Results
The SEM showed differences between the surfaces. BIC of the LHS implants were statistically superior to MS at the 2-week period and LHS and LS surfaces were statistically superior to MS at the 4-weel period. Statistical analysis of the NBA of the implants showed difference between the LHS and MS surface in the period of 2 weeks.
Conclusion
In conclusion, the modifications of the surfaces LS and LHS provided important physicochemical modifications that favored the deposition of bone tissue on the surface of the implants.
Clinical relevance
Surface modifications by laser with and without HA in dental implant surfaces accelerated osseointegration process and it is indicated oral rehabilitation in bone compromised.
The Szeged Index of Power Graph of Finite Groups
Authors: Banerjee Subarsha 
Published: September 2023

Abstract
The Szeged index of a graph is an invariant having several applications in chemistry. The power graph of a finite group G is a graph having vertex set as G in which two vertices u and v are adjacent if v = u m or u = v n for some m, n ∈ N. In this paper, we first obtain a formula for the Szeged index of the generalized join of graphs. As an application, we obtain the Szeged index of the power graph of the finite cyclic group Z n for any n > 2. We further obtain a relation between the Szeged index of the power graph of Z n and the Szeged index of the power graph of the dihedral group D n. We also provide SAGE codes for evaluating the Szeged index of the power graph of Z n and D n at the end of this paper.
2020 Mathematics Subject Classification: 05C09, 05C25, 05C50.
Highly coherent flat supercontinuum generation in highly nonlinear silica fiber pumped by 1.5μm femtosecond fiber laser
Authors: Xu Zipeng , Wang Xuan , Yao Chuanfei , Yang Linjing , Li Pingxue 
Published: September 2023

Abstract
Supercontinuum light sources with both highly flatness and highly coherence have unique application advantages in biological imaging, optical frequency comb, and optical coherence tomography. We demonstrate the generation of a highly coherent flat supercontinuum spectrum. In order to achieve broadband flat spectrum generation, we used a highly nonlinear fiber with a nominal dispersion of zero at 1550 nm and a dispersion slope of less than 0.025 ps/(nm2·km). Meanwhile a sub 100 fs fiber laser was used as the pump source in the experiment to achieve good coherence. When the pump power was 134 mW, the supercontinuum emission was characterized between 1197 nm and 2242 nm, showing the flat spectrum with 10 dB uniformity was obtained at the wavelength region of 1603–2242 nm and wide spectrum with a conversion efficiency over 85%. The coherence of the supercontinuum spectrum was 1 in the range of 1200–2242 nm by theoretically studying the coherence characteristics of the supercontinuum spectrum. In addition, the effect of pump laser duration on spectral flatness was demonstrated through experiments. Our experiment provides a feasible method for the generation of near-infrared flat highly coherence supercontinuum spectrum with all optical fibers structure.
Examining Planetary Boundary Layer Height Climatology, Trends, and Variability over India using High-Resolution Indian Reanalysis
Authors: Shukla Krishna Kumar , Attada Raju , Pathaikara Akash 
Published: September 2023

Abstract

This work investigates the spatio-temporal variability of planetary boundary layer height (PBLH) characteristics by leveraging multi-decadal (1980–2019) data from India’s first high-resolution regional atmospheric reanalysis–IMDAA, in conjunction with ERA5 and MERRA-2. The spatial variability in the seasonal and annual climatological mean PBLH obtained from IMDAA agree well with ERA5 and MERRA-2, albeit with some inconsistencies. The IMDAA and ERA5 PBLH exhibits a high correlation (> 0.6) over entire India, and is also exhibits a significant positive (negative) correlation with MERRA-2 over northwest and central (southern and eastern) Indian regions. However, IMDAA tends to overestimate ERA5 PBLH ( ~ < 500 m) and underestimate MERRA-2 PBLH ( ~ > 500 m) during all seasons. Despite these discrepancies, IMDAA successfully captures the diurnal changes in PBLH similar to ERA5 and MERRA-2. Furthermore, the evaluation of IMDAA PBLH with other meteorological factors suggests that PBLH correlates negatively with relative humidity (RH), indicating a decrease in PBLH as RH increases. On the other hand, PBLH shows positive correlations with surface temperature and surface zonal winds. Surface sensible and latent heat flux exhibit positive and negative correlations with PBLH, respectively, over Indian sub-regions throughout all seasons. Moreover, IMDAA realistically represents the declining trend of PBLH (-1.1 to -76.2 m decade− 1) compared to ERA5 in India during all seasons. The results from IMDAA, in concurrence with other reanalyses, demonstrate that the decreasing trend in PBLH over India is associated with rising surface temperatures and weakening surface zonal winds. This trend is attributed to increasing latent heat flux and decreasing sensible heat flux. The changes in surface fluxes over India are attributed to the intensification of Indian monsoon rainfall in the last three decades. Moreover, El Niño appears to be an important control on PBLH variability over India during different seasons, which is realistically represented by IMDAA as in ERA5 and MERRA-2.
Development of Chromium-Impregnated Sulfated Silica as a Mesoporous Catalyst in the Production of Biogasoline from Used Cooking Oil via a Hydrocracking Process
Authors: Ningrum Tyas Sekar , Saviola Aldino Javier , Wijaya Karna , Prasetyo Niko , Ardelia Zulfaa Lola , Fitria Riska Astin , Gea Saharman , Hauli Latifah , Amin Amalia Kurnia , Saputri Wahyu Dita , Setiawan Adi , Oh Won-Chun 
Published: September 2023

Abstract

The production of biofuels as an alternative to fossil fuels has been a significant challenge until recently. The present work focuses on hydrocracking used cooking oil (UCO) into biogasoline over chromium impregnated on a sulfated mesoporous silica catalyst. The effects of varying sulfuric acid concentration, calcination temperature, and impregnated chromium content (wt%) were systematically studied in the synthesis process employing TEOS and NaHCO3 by sol-gel method. A sulfuric acid concentration of 2 M and calcination temperature of 600 ˚C produced an SO4-SiO2 catalyst with the best acidity of 8.46 mmol g-1. Variation of chromium content (wt%) of 1% had Cr/SO4-SiO2 catalyst with the best acidity of 8.57 mmol g-1. SiO2, SS 2-600, and Cr-SS 1 catalyst were tested for their performance in the hydrocracking of UCO into biogasoline at an optimum temperature of 450 °C, H2 gas flow rate of 20 mL min-1, and catalyst-to-feed ratio (wt%) of 1:100. Hydrocracking using Cr-SS 1 catalyst produced the most liquid product of 37.14% with the highest gasoline fraction selectivity of 29.38%.
The association between blastocyst quality during a single frozen-thawed blastocyst transfer cycle and subsequent perinatal outcomes: a propensity score-matched study
Authors: Wu Jing , Yang Chen , Zheng Wei , Cao Meng Yi , Li Meng Na , Kuang Yi Hui , Ren Bing Nan , Ran Shi Yu , Zu Ruo Wen , Wu Huan , Shen Ran , Han Jing Yi , Li Pei Xin , Guan Yi Chun 
Published: September 2023

Abstract
Background
At present, with the increasing acceptance of blastocyst transfer, more and more attention were being paid to the selection of blastocysts during the transplant cycle. The preferred method for reducing the incidence of adverse perinatal outcomes was to select blastocysts with a low rate of adverse perinatal outcomes for transplantation. However, few studies have investigated the relationship between blastocyst quality and perinatal outcomes in single frozen-thawed blastocyst transfer (SFBT) cycles. The aim of this study was to focus solely on the blastocyst and investigate the association between embryo morphological grading and perinatal outcomes in a single frozen-thawed blastocyst transfer (SFBT) cycle.
Method
In a retrospective case-control study, we divided patients into the good-quality blastocysts transfer group and the poor-quality blastocyst transfer group based on the Gardner and Schoolcraft system. To control for potential confounding factors, propensity score-matching (PSM) was used to match male age, secondary infertility, primiparity, developmental day of the transferred blastocysts, endometrial thickness on transfer day, and polycystic ovary syndrome. Patients who received single vitrified-warmed high-quality blastocysts were matched with patients who received single poor-quality blastocysts at a 1:1 ratio.
Result(s)
Both before and after using PSM, there were no statistically significant differences in obstetric outcomes, including the incidence of gestational hypertension, gestational diabetes, or cesarean delivery, among women receiving SFBT with different blastocyst quality. There were also no significant differences in neonatal outcomes between the two groups with respect to newborn birth weight or the occurrence of birth defects.
Conclusion
The quality of blastocysts did not affect the perinatal outcomes of SFBT cycles. It is feasible to select a single poor-quality blastocyst for SFBT.
Increasing trend of angiogenic indices following Traditional and Circuit Resistance Training in sedentary men
Authors: Gharaat Mohammad Ali , MehriAlvar yaghoob , sheykhlouvand Mohsen 
Published: September 2023

Abstract

Background Angiogenesis is a beneficial process leading to capillary enhancement, which can trigger in response to physical training. Aims The present study aimed to investigate the changes in physiological factors involved in angiogenesis in response to circuit or traditional resistance training. Methods Following the pre-test, Thirty-six healthy sedentary students (age: 21.1 ± 2.3 years; height: 172.7 ± 5.1 cm) were randomly divided into Circuit Resistance Training (CRT) (4 circles/3 times a week/8 weeks circuit performance/50–55% 1 Repetition Maximum;n = 12), Traditional Resistance Training (TRT) (8 repetition/3 set/3 time per week/8 weeks of same movements with 75% 1RM followed by 2 minutes break between the sets;n = 12) and the control group (n = 12). We examined the responses of Vascular Endothelial Growth Factor (VEGF), plasma level of growth hormone (GH), and basic Fibroblast Growth Factor (bFGF). Results Both of CRT and TRT protocols resulted in significant increases in post-test compared to pre-test, in VEGF (p = 0.00), GH (p = 0.04), and bFGF (p = 0.00). In addition, the magnitude of changes in VEGF and GH were significantly greater than the control group in post-test (p = 0.03, and 0.001, respectively). In addition, there was a strong correlation between absolute value of GH and VEGF (r = 0.74 and r = 0.71) following CRT (p = 0.01) and TRT (p = 0.02). Conclusions This study demonstrated that CRT and TRT might enhance angiogenesis through increase in VEGF, bFGF and GH.
A fine-tuned machine learning model to predict survivals of breast cancer patients based on gamma-delta T cell markers
Authors: Zhou Lina , Weng Jia , Ding Xiao , Zhuang Zhixiang , Fan Rencai , Zhang Jiaqi , Chen Lei , Li Shicheng 
Published: September 2023

Abstract
Background: Gamma-delta (γδ) T cells influence cancer immunotherapy and prognosis by enhancing clinical responses to checkpoint inhibitors. However, identifying prognostic markers for γδ T cells remains a crucial challenge.
Methods: Initially, we identified γδ T cell markers specific to breast cancer (BC) through single-cell analysis of GSE195861 dataset from the GEO database. Subsequently, we utilized LASSO regression to select prognostic genes for use as variables in artificial intelligence (AI) models. We performed survival analysis, assessed cancer microenvironment scores, and conducted biological functional analysis on these selected genes. Following this, we trained five commonly used machine learning models, and the best-performing model in the training set underwent careful tuning to optimize its performance. Tissues from five GEO datasets and our hospital were used for model validation.
Results: Cells of BC were classified into 12 subclasses, and the γδT cells accounted for the majority. Totally, 310 markers of γδT cells were identified, among which 12 were proved to have the prognostic ability by the external validation of GSE20685 (Hazard Ratio (HR) = 1.634, p = 0.031), GSE3143 (HR = 2.887, p = 0.001), GSE19536 (HR = 2.713, p = 0.001), GSE202203 (HR = 2.088, p < 0.001) and GSE35629 (HR = 6.476, p = 0.001). The random forest (RF) model with the 10-fold cross-validation achieved the best accuracy of 0.824 among the trained models. Then performance of the RF model increased to the accuracy of 0.835 after fine-tuned. In the validation, the AUC /accuracy of the fine-tuned RF model is 0.81/0.849 in GSE20685, 0.75/ 0.812 in GSE3143, 0.75/ 0.807 in GSE19536, 0.80/ 0.841 in GSE202203, 0.78/ 0.821 in GSE35629 and 0.77/0.801 in the real-world cohort.
Conclusions: We developed an efficient machine learning model based on γδT cell markers, which had a potent capability to predict the prognosis of BC patients.
On Strongly m-Convex Stochastic Processes
Authors: Bisht Jaya , Mishra Rohan , Hamdi Abdelouahed 
Published: September 2023

Abstract
In this paper, we introduce the concept of strongly m-convex stochastic processes and present some basic properties of these stochastic processes. We derive the Hermite-Hadamard type inequalities for stochastic processes whose first derivatives in absolute values are strongly m-convex. The results presented in this paper are the generalization and extension of the previously known results.
Mathematics Subject Classification (2010). 26A51, 26D15, 52A01, 60G99.
Multidimensional human wellbeing in periodic octopus closures
Authors: O'Neill Elizabeth Drury , Daw Tim M. , Lindkvist Emilie , MWAMBAO  , Martínez Jineth Berrío , Wamukota Andrew , Mwaipopo Rosemarie 
Published: September 2023

Abstract

This paper investigates multidimensional human wellbeing in periodic octopus closures, a common type of marine protected area (MPA) in the western Indian ocean. We trace the perceived flow of benefits, costs and trade-offs from closures at three sites in Zanzibar. We specifically explore how different types of fishers and traders perceive the impacts of closures on wellbeing from a personal, livelihood group and village or community level, as well as ecosystem effects. Our interpretive methods which prioritized participants' emic descriptions and understandings of closure impacts. We iteratively, qualitatively coded data using a three-dimensional (material, relational and subjective) social wellbeing approach. Despite different conditions and histories at the three sites, participants identified similar wellbeing attributes as affected by the closure. Themes included social conflict, non-compliance, income, education, food/nutrition, perceptions of inequity and communal benefits reflecting recent literature on MPAs and human wellbeing Gender was a strong dimension that emphasized procedural and distributional inequity between different types of livelihood groups e.g. small-scale traderwomen and male skindivers. Material wellbeing losses due to poor market environments highlighted how better alignment is needed between periodic closure activities and resulting trade and market dynamics. Opening events thoroughly impacted wellbeing across all dimensions, suggesting that these moments are critical for creating positive perceptions or losing support for closures.
Compliance, Complexity and Cephalopods- Disaggregated Responses to Participatory Marine Conservation &amp; Management
Authors: O'Neill Elizabeth Drury , Daw Tim M. , Lindkvist Emilie , MWAMBAO  , Mwaipopo Rosemarie 
Published: September 2023

Abstract

Breaking of rules, whether formal regulations, or community agreed norms, remains one of the most widespread challenges to sustainable use of marine ecosystems. Non-compliance is especially difficult in small-scale fisheries as it can undermine the provision of nutrition and livelihoods to the many millions dependent worldwide. Although fishery compliance scholarship today is expanding beyond non-economic explanations of (non)-compliance behaviour, approaches and theory used fail to show the diversity of perceptions and dispositions that can underpin motivations. Thus a fuller understanding is lacking towards addressing the acute need for increased fisheries compliance. This paper takes a Zanzibari case of a rapidly spreading community-based intervention - periodic octopus closures - to explore compliance outcomes amongst a diverse range of participants. We take a qualitative approach aligning with an interpretive paradigm to carry out story circles, photo elicitation tasks and focus groups at three sites in 2019. Two sociological strands of theory were used to interpret how participants justify, are motivated by or dispositioned towards rules, rule-makers and rule-breakers. Generally the intervention was supported, signalling the potential for future compliance most commonly based on recognition of a degraded marine ecosystem in need of protection. However the diverse and dynamic responses by groups indicated on which basis and through which logics compliance behaviour was condoned or legitimated. This type of insight can aid managers gauge or anticipate the potential for non-compliance in participatory approaches that can impact the health of marine ecosystems.