bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell division protein FtsK coordinates bacterial chromosome segregation and daughter cell separation in Staphylococcus aureus
Authors: Veiga Helena , Jousselin Ambre , Schäper Simon , Saraiva Bruno M , Marques Leonor B , Reed Patricia , Wilton Joana , Pereira Pedro M , Filipe Sérgio R , Pinho Mariana G 
Published: April 2023

AbstractUnregulated cell cycle progression may have lethal consequences and therefore, bacteria have various mechanisms in place for the precise spatiotemporal control of cell cycle events. We have uncovered a new link between chromosome replication/segregation and splitting of the division septum. We show that the DNA translocase domain‐containing divisome protein FtsK regulates cellular levels of a peptidoglycan hydrolase Sle1, which is involved in cell separation in the bacterial pathogen Staphylococcus aureus. FtsK interacts with a chaperone (trigger factor, TF) and establishes a FtsK‐dependent TF concentration gradient that is higher in the septal region. Trigger factor binds Sle1 and promotes its preferential export at the septal region, while also preventing Sle1 degradation by the ClpXP proteolytic machinery. Upon conditions that lead to paused septum synthesis, such as DNA damage or impaired DNA replication/segregation, TF gradient is dissipated and Sle1 levels are reduced, thus halting premature septum splitting.
Dedifferentiation‐derived neural stem cells exhibit perturbed temporal progression
Authors: Veen Kellie , Nguyen Phuong‐Khanh , Froldi Francesca , Dong Qian , Alvarez‐Ochoa Edel , Harvey Kieran F , McMullen John PD , Marshall Owen , Jusuf Patricia R , Cheng Louise Y 
Published: April 2023

AbstractDedifferentiation is the reversion of mature cells to a stem cell‐like fate, whereby gene expression programs are altered and genes associated with multipotency are (re)expressed. Misexpression of multipotency factors and pathways causes the formation of ectopic neural stem cells (NSCs). Whether dedifferentiated NSCs faithfully produce the correct number and types of progeny, or undergo timely terminal differentiation, has not been assessed. Here, we show that ectopic NSCs induced via bHLH transcription factor Deadpan (Dpn) expression fail to undergo appropriate temporal progression by constantly expressing mid‐temporal transcription factor(tTF), Sloppy‐paired 1/2 (Slp). Consequently, this resulted in impaired terminal differenation and generated an excess of Twin of eyeless (Toy)‐positive neurons at the expense of Reversed polarity (Repo)‐positive glial cells. Preference for a mid‐temporal fate in these ectopic NSCs is concordant with an enriched binding of Dpn at mid‐tTF loci and a depletion of Dpn binding at early‐ and late‐tTF loci. Retriggering the temporal series via manipulation of the temporal series or cell cycle is sufficient to reinstate neuronal diversity and timely termination.
Analisis Produksi Microgreens Brassica oleracea Berinovasi Urban Gardening Untuk Peningkatan Mutu Pangan Nasional
Authors: Febriani Vina , Nasrika Evy , Munasari Tri , Permatasari Yoan , Widiatningrum Talitha 
Published: April 2023

Peningkatan jumlah penduduk membuat lahan yang tersedia menjadi semakin sempit, hal ini berdampak pada kurangnya lahan yang tersedia untuk menanam tanaman karena tergusur oleh kebutuhan pemukiman, masyarakat membutuhkan tanaman organik sebagai bahan untuk dikonsumsi. â€œMba-Geningâ€ : Microgreens Brassica oleracea Berinovasi Urban Gardening hadir sebagai salah satu solusi minimnya lahan untuk menanam. Microgreens adalah tanaman herba yang dipanen di usia muda, yaitu 10-14 hari atau setelah melewati proses perkecambahan. Tanaman microgreens biasa dipanen dengan tinggi 5-7,5 cm. Microgreens dibudidayakan padaÂ trayÂ khusus dengan media tanam. Benih microgreens di sebar ke media tanam, diberi air dan di-sprayÂ 1-2 kali sehari untuk pertumbuhannya. Microgreens memiliki kandungan nutrisi lebih banyak dari pada sayuran hijau yang dipanen saat dewasa, yaitu mencapai hingga 6 kali lipatnya. Microgreens dapat dikonsumsi secara langsung tanpa dimasak, umumnya, digunakan sebagaiÂ garnishÂ atau hiasan pada masakan di restoran, sepertiÂ steakÂ dan sup, dapat pula dikonsumsi sebagai salad dan isianÂ sandwich. Produksi Mba-Gening dapat menjawab kebutuhan masyarakat akan sayur organik khususnya brokoli, selain itu juga membuang peluang usaha yang baik karena memiliki target pasar yang luas serta belum ada kompetitor di pasaran. Produksi dan teknik pemasaran yang baik dapat meningkatkan hasil dari usaha ini. Kedepannya, produk akan ditingkatkan kualitasnya sesuai dengan kebutuhan konsumen agar kepuasan konsumen semakin meningkat.
In Vitro Biological Activities of Drepanoalpha® Ethanolic Extract, A Justicia Secunda and Moringa Oleifera-Based Phytomedicine Proposed for The Symptomatic Treatment of Sickle Cell Disease
Authors: Gbolo Zoawe Benjamin , Nachtergael Amandine , Tshibangu Damien S. T. , Misengabu Nicole M. , Nsabatien Victoire , Memvanga Patrick B. , Tshilanda Dorothée D. , Ngbolua Jean-Paul K. N. , Mpiana Pius T. , Duez Pierre 
Published: April 2023

Sickle cell disease (SCD) is an autosomal recessive blood disorder characterized by red blood cells that assume an abnormal, rigid sickle shape under low-oxygen conditions. These sickle-shaped erythrocytes tend to lyse, aggregate, and obstruct small blood vessels, leading to major complications. The present study aims to investigate properties that may underlie the activity of Drepanoalphaâ, an antisickling herbal formulation developed in the Democratic Republic of Congo (DRC) for the prevention and symptomatic treatment of sickle cell disease crises. The Drepanoalpha® Ethanolic Extract (DEE) is a dry extract (drug-extract ratio, DER, 100/11) prepared from ethanol (96 %, v/v) percolation of a 1:1 mixture of 2 food plants, Justicia secunda Vahl and Moringa oleifera Lam. Sickling was classically measured by light microscopy on diluted washed erythrocytes obtained from homozygote patients; erythrocytes were treated with 2 % Na2S2O5 in the presence of DEE (suspension in 9 ‰ NaCl), 9 ‰ NaCl (negative control) or disodium cromoglycate (DSCG, positive control). For all tested conditions, the sickle hemoglobin polymerization, the Fe2+/Fe3+ ratio, and the median corpuscular fragility were measured by spectrophotometry. The DEE reversed sickling by 89.1 %, comparable to DSCG (87.7 %; 60.3 µg/mL), inhibiting sickle cell hemoglobin polymerization of 77.8 % and 74.4 %, respectively. The Fe2+/Fe3+ ratio was improved by 18.0 % for DEE and 15.9 % for DSCG. The median corpuscular fragility values were 0.602, 0.714, and 0.732 for NaCl 9 ‰, DSCG, and DEE, respectively. The measured in vitro parameters validate an effective antisickling effect of DEE and confirm the value of this improved traditional herbal formulation for the management of SCD.
Análisis DFT de fosforeno y fosforeno oxidado como materiales adsorbentes de Cu2+ a partir de una solución acuosa
Authors: Florez Elizabeth , Correa Julián 
Published: April 2023

Se hizo un estudio sistemático utilizando la teoría de los funcionales de la densidad (DFT) para lograr una mejor comprensión del papel de la concentración de oxígeno en el fosforeno prístino durante la adsorción de Cu2+ en sistemas acuosos. La caracterización electrónica del fosforeno y del fosforeno oxidado se calculó a partir de la brecha de energía y la dureza química. Los resultados permitieron concluir que los sistemas oxidados presentaron una brecha de energía y una dureza menores que las del sistema prístino. Además, a medida que aumentó la concentración de oxígeno, estos valores decrecieron. La interacción del Cu2+ con las diferentes superficies se caracterizó utilizando cargas atómicas, el índice de enlace y espectroscopia fotoelectrónica de rayos-X (XPS). Los valores de la energía de adsorción indicaron que cuando el fosforeno está oxidado, la interacción con el Cu2+ fue más fuerte comparada con la de la superficie prístina. Asimismo, el aumento en la concentración de oxígeno mejoró las capacidades del fosforeno como adsorbente, lo cual se relaciona con la facilidad que tiene este sistema para la transferencia hacia el Cu2+ dados los reducidos valores de la brecha de energía y la dureza química. Nuestros resultados contribuyen a una mejor comprensión del efecto de la concentración de oxígeno en la superficie de fosforeno en la adsorción de Cu2+, lo que respalda la idea de que este tipo de materiales bidimensionales (2D) tiene uso potencial en la remoción de metales pesados de las aguas residuales.
Security Technologies Using Facial Recognition
Authors: Salama Ramiz , Nour Mohamed 
Published: April 2023

Abstract

Faces are one of the simplest methods to determine a person's identity. Face recognition is a unique identifying method that uses an individual's traits to determine the identity of that individual. The proposed recognition process is divided into two stages: face recognition and object recognition. Unless the item is very close, this procedure is very rapid for humans. The recognition of human faces is introduced next. The stage is then reproduced and used as a model for facial image recognition (face recognition). That's one of the professionally created and well-researched biometrics procedures. The eigenface approach and the Fisher face method are two common face recognition pattern algorithms that have been developed. Recognition of facial images The Eigenface approach is based on the reduction of face dimensional space for facial traits using Principal Component Analysis (PCA). The major goal of applying PCA on face recognition was to generate Eigen faces (face space) by identifying the eigenvector corresponding to the face image's biggest eigenvalue. Image processing and security systems are areas of interest in this research face recognition integrated into a security system.

Keywords: face recognition, security systems, camera, python;
Information Security for Small Business Course Proposal and Design
Authors: Xu Wenjuan 
Published: April 2023

These days small businesses deploy different information systems to be more competitive and efficient, at the same time , which leads to different cyber attacks. To reduce the possible infrastructure damage and financial loss, business owners need to be aware of and recognize these various attacks, and know how to reduce or prevent them.
In this paper, to help with the local small business owners, we propose an information security course especially designed for educating the business owners in cyber security for small business-related fields. This paper examines the course design details including the course structure, main contents, different exercises and main challenges.
Challenges and strategies to stay physically active in the face of COVID-19 pandemic: A review
Authors: Camilo Bruno F. , Baranowski-Pinto Gabriela , Lopes Filho Brandel J. P. , Cristina-Souza Gislaine 
Published: April 2023

The pandemic caused by the COVID-19 has influenced peoples’ lifestyles. Home-confinement scenario might impair physical activity practice, resulting in new challenges for maintaining health during the pandemic of the COVID-19. The aim of this study was to present the current context of COVID-19 pandemic, its impact on the practice of physical activity, and the strategies available to remain active during home-confinement according to international recommendations. The narrative review was conducted based on studies that analyzed themes related to physical activity and COVID-19. Virtual Health Library (VHL), CINAHL, Cochrane, PsycINFO, PubMed, ScienceDirect, Scientific Electronic Library Online (SciELO), Scopus, SPORTDiscus, and Web of Science databases were searched for relevant papers. Although an increased number of experimental studies are still necessary, people should devote more time to physical activity during social isolation. Guidelines were adjusted by international entities in order to encourage people to remain active, through practice regular physical activity, using alternative strategies such as fitness program applications, exergames, online exercise classes, and even chores. Reduction of prolonged sedentary behavior could contribute to maintain health and improve quality of life during the COVID-19 pandemic.
Uji Antioksidan dan Karakterisasi Minyak Atsiri dari Kulit Jeruk Purut (Citrus hystrix D. C)
Authors: Latifah Fadzil , Taufiq Hudan , Fitriyana Nur Maulida 
Published: April 2023

<span lang="EN-US">Minyak atsiri banyak digunakan sebagai bahan baku kosmetik, salah satunya minyak atsiri kulit jeruk purut sebagai antioksidan. Berkembangnya pasar minyak atsiri menjadi faktor pendorong pemalsuan karena hasil produksi rendah, tidak </span><span lang="EN-US">adanya standar kualitas minyak atsiri yang diproduksi ditandai dengan sulitnya dalam mengidentifikasi produk yang dipalsukan. Tujuan penelitian ini untuk mengetahui aktivitas antioksidan (nilai IC<sub>50</sub>) dan karakteristik minyak atsiri kulit jeruk purut sebagai acuan standar kualitas. Kulit buah jeruk purut disuling menggunakan metode penyulingan uap dan air. Aktivitas antioksidan sampel minyak atsiri kulit jeruk purut dan trolox diuji menggunakan metode CUPRAC. Persen rendemen, total minyak, kecerahan, berat jenis, indeks bias, rotasi optik, bilangan asam, kelarutan dalam alkohol, bilangan ester, dan analisis senyawa dengan GC-MS. Hasil aktivitas antioksidan minyak atsiri kulit jeruk purut dan kontrol positif trolox terdapat perbedaan signifikan (p≤0,05), dengan nilai IC<sub>50</sub> 23,4182 ppm dan 53,8605 ppm. Minyak atsiri kulit jeruk purut memiliki persen rendemen 1,86045%, tidak berwarna, berat jenis 0,84028 g/ml pada suhu 25°C, nilai indeks bias 1,4710, rotasi optik +12,70, bilangan asam 0,8415, larut dalam 1:6 bagian alkohol 90%, bilangan ester 19,635 serta mengandung 25 senyawa dengan tiga senyawa tertinggi β-pinene, limonene, sabinene. Aktivitas antioksidan minyak atsiri kulit jeruk purut tergolong sangat kuat dan karakteristik sesuai standar. </span>
Autonomous learning practices of extrovert and introvert EFL learners
Authors: Savitri Wiwiet Eva 
Published: April 2023

Learning autonomy has been widely seen as a key predictor of a language learning success. There have been many studies on learning autonomy, however, most studies do not investigate how students conduct their autonomous learning. The number of those which investigate how learning autonomy is implemented across personality type is even fewer. To address it, this article will elaborate how extrovert and introvert students managed their autonomous learning. The objectives of this article are to reveal how extrovert and introvert learners perceive autonomous learning and to know the types of activities which were done by the extrovert and introvert learners and their reasons for doing the activities. Interviews and observation were applied to collect data. The findings indicate that autonomous learning was carried out differently by both personality types. Introvert students devoted more time and focused on their solitude learning and they are more organized in doing it. On the other hand, extrovert learners chose to do the autonomous learning in groups. For them, learning in a community gave more benefits. One of the benefits was preventing them from making fossilized errors. This article also reveals that the students from both personality types used digital technology in learning independently. Yet, extrovert and introvert students used different kinds of technology and way in using it.
HIGHLIGHTS:

Autonomous learning does not merely mean learning alone because it refers to self-triggerto learn, both individually and in groups
Extrovert and introvert learners have different perception and apply different strategiesin implementing autonomous learning.
Inductive Type Impedance of Mo/<i>n</i>-Si Barrier Structures Irradiated with Alpha Particles
Authors: Poklonski N. A. , Kovalev A. I. , Usenko K. V. , Ermakova E. A. , Gorbachuk N. I. , Lastovski S. B. 
Published: April 2023

In silicon microelectronics, flat metal spirals are formed to create an integrated inductance. However, the maximum specific inductance of such spirals at low frequencies is limited to a value of the order of tens of microhenries per square centimeter. Gyrators, devices based on operational amplifiers with approximately the same specific inductance as spirals, are also used. Despite the fact that such solutions have been introduced into the production of integrated circuits, the task of searching for new elements with high values of specific inductance is relevant. An alternative to coils and gyrators can be the effect of negative differential capacitance (i.e., inductive type impedance), which is observed in barrier structures based on silicon.The purpose of the work is to study the low-frequency impedance of Schottky diodes (Mo/n-Si) containing defects induced by α-particles irradiation and determination of the parameters of these defects by methods of low-frequency impedance spectroscopy and DLTS (Deep Level Transient Spectroscopy).Unpackaged Schottky diodes Mo/n-Si (epitaxial layer of 5.5 μm thickness and resistivity of 1 Ohm∙cm) produced by JSC “Integral” are studied. Inductance measurements were carried out on the as manufactured diodes and on the diodes irradiated with alpha particles (the maximum kinetic energy of an αparticle is 5.147 MeV). The impedance of inductive type of the Schottky diodes at the corresponding DC forward current of 10 µA were measured in the AC frequency range from 20 Hz to 2 MHz. DLTS spectra were used to determine the parameters of radiation-induced defects. It is shown that irradiation of diodes with alpha particles produces three types of radiation-induced defects: A-centers with thermal activation energy of E1 ≈ 190 meV, divacancies with activation energies of E2 ≈ 230 meV and E3 ≈ 410 meV, and Ecenters with activation energy of E4 ≈ 440 meV measured relative to the bottom of c-band of silicon.
Calculation of Parameters of Prism Deflector for Laser Scanner
Authors: Gusarov I. E. , Kalugin A. I. , Alyes M. Yu. , Antonov E. A. 
Published: April 2023

The prism deflector which is a multifaceted prism with reflective facets is the most common scanning element that allows to quickly fill a wide scanning area with laser radiation pulses along one coordinate. The parameters of a prismatic deflector are related to the characteristics of the laser radiation of the scanned area parameters and of the deflector position, and are also limited by various factors, such as safety requirements or scanning time. The aim of this work was to analyze the relationship between the external operating conditions of a laser scanning system and the internal design parameters of a prism deflector.A variant of calculating of laser pulses frequency by the number of spots and their overlap coefficient is considered. A method for calculating of a prism deflector characteristics based on external parameters, such as the angle of incidence of radiation to the facet and the width of the Gaussian beam with dimensions that are safe for а human eye is proposed. Рrismatic deflectors parameters are proposed depending on the number of reflecting facets. Dependence of a deflector size on the angle of radiation incidence to the reflecting face is shown.When designing a prismatic deflector of a laser scanner used to fill a certain scanning area with the required angular size σ by varying such parameters as the number of faces m and the feed angle α it is possible to achieve optimal deflector characteristics and scanning mode for the task.
Purpose and Justification of Traditional Modes of Heat Treatment of Concrete and Reinforced Concrete Products
Authors: Zelenkovskaya Zh. L. , Kovshar S. N. 
Published: April 2023

The paper presents the comparative characteristics of the most common modes of heat and moisture treatment (HMT), their advantages and disadvantages, as well as proposals for optimizing the HMT stages to obtain the maximum effect of accelerating concrete hardening through the use of thermal energy. Heat and moisture treatment of concrete is one of the most difficult stages in the technology of prefabricated and monolithic concrete. The basis for the durability of structures, their uninterrupted service during the design period of operation is a properly selected HMT mode, which improves the quality of products and reduces material costs in the form of a reduction in energy costs. Therefore, the still practiced simplified methods for selecting the HMT mode are unacceptable. Only under the condition of strict and scientifically substantiated consideration of a complex of factors influencing the ongoing processes of formation of the structure of cement stone and concrete, and the interaction between them, it is possible to obtain concrete with the required characteristics. Depending on the requirements for the finished material, based on knowledge of the mechanism of heat and mass transfer, rational methods and modes of heat treatment of concrete and reinforced concrete products can be calculated. A variety of HMT modes is due to the desire to reduce the possibility of defects in the concrete structure (for example, modes with a stepped or curvilinear temperature increase, which reduces the temperature gradient across the product section), to reduce energy costs (modes with the exclusion of the isothermal holding stage), etc. In the process of HMT of concrete and reinforced concrete products, a number of chemical and physical transformations of the concrete mixture (concrete) occur, as a result of which various defects in the structure of the material may appear, which worsen its properties (strength, permeability, shrinkage, creep and, in general, durability of concrete). Modern technology for the production of concrete and reinforced concrete products and structures provides for the introduction of various chemical additives, their effect on the hardening of concrete at elevated temperatures, unfortunately, is not sufficiently reflected in the specialized literature. For example, the duration of the total cycle of concrete HMT when using chemical additives – hardening accelerators can be reduced by reducing the periods of preliminary exposure, temperature rise and the duration of isothermal exposure; and the use of plasticizers, depending on their type and content, can lead to a lengthening of the cycle. It is necessary to have analytical dependencies for calculating HMT modes and a computer model of the concrete hardening process at elevated temperatures.
Forced Quadcopter Control
Authors: Gu Penghao , Leonovets J. A. , Lobaty A. A. 
Published: April 2023

The problem of analytical synthesis of the control acceleration of an unmanned aerial vehicle (UAV) of a multirotor type is being solved in relation to a light quadrocopter with a high flight duration. The optimal control is analytically determined for a given minimized quality functional in the form of the minimum time required to transfer the UAV from a given initial to a given final position in space. A mathematical model of the movement of the UAV mass center in a given plane relative to the earth's surface is considered. A feature of the proposed technique is the solution of the problem of maximum speed (forced control) based on the consideration of the laws of kinematics of uniformly accelerated motion of a rigid body. For given characteristics of the maximum allowable speed and control acceleration of the UAV, the moments of switching of the control signal are analytically calculated, which can be implemented in the UAV autopilot. This allows, in contrast to classical methods for solving the problem of forced control, to get rid of the need to solve a two-point boundary value problem and consider additional transversality conditions. The computer simulation of the obtained analytical results in the form of processes of changing the control acceleration, as well as the UAV motion parameters, has shown the efficiency of the proposed technique and the prospects for its use at the initial stage of the synthesis of the UAV control system.