bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of nanoparticle encapsulation and encoding on the surface chemistry of polymer carrier beads
Authors: Scholtz Lena , Tavernaro Isabella , Eckert J. Gerrit , Lutowski Marc , Geißler Daniel , Hertwig Andreas , Hidde Gundula , Bigall Nadja C. , Resch-Genger Ute 
Published: July 2023

AbstractSurface-functionalized polymer beads encoded with molecular luminophores and nanocrystalline emitters such as semiconductor nanocrystals, often referred to as quantum dots (QDs), or magnetic nanoparticles are broadly used in the life sciences as reporters and carrier beads. Many of these applications require a profound knowledge of the chemical nature and total number of their surface functional groups (FGs), that control bead charge, colloidal stability, hydrophobicity, and the interaction with the environment and biological systems. For bioanalytical applications, also the number of groups accessible for the subsequent functionalization with, e.g., biomolecules or targeting ligands is relevant. In this study, we explore the influence of QD encoding on the amount of carboxylic acid (COOH) surface FGs of 2 µm polystyrene microparticles (PSMPs). This is done for frequently employed oleic acid and oleylamine stabilized, luminescent core/shell CdSe QDs and two commonly used encoding procedures. This included QD addition during bead formation by a thermally induced polymerization reaction and a post synthetic swelling procedure. The accessible number of COOH groups on the surface of QD-encoded and pristine beads was quantified by two colorimetric assays, utilizing differently sized reporters and electrostatic and covalent interactions. The results were compared to the total number of FGs obtained by a conductometric titration and Fourier transform infrared spectroscopy (FTIR). In addition, a comparison of the impact of QD and dye encoding on the bead surface chemistry was performed. Our results demonstrate the influence of QD encoding and the QD-encoding strategy on the number of surface FG that is ascribed to an interaction of the QDs with the carboxylic acid groups on the bead surface. These findings are of considerable relevance for applications of nanoparticle-encoded beads and safe-by-design concepts for nanomaterials.
A new hollow solenoid receiver compatible with the global double-D transmitter for EV inductive charging
Authors: Shaier Ahmed A. , Mohamed Ahmed A. S. , Metwally Hamid , Selem Sameh I. 
Published: July 2023

AbstractInductive power transfer (IPT) technology is a promising solution for charging the electric vehicles (EVs) by enabling an EV to charge its energy storage system (battery) without any connecting wires through magnetic coupling. This paper proposes a new receiver design named Hollow Solenoid receiver that is compatible with the standard double-D transmitter defined by the SAE J2954 standard. A deep design analysis for the proposed Hollow Solenoid receiver is presented to define the optimum design parameters for coil (inductances, number of turns, dimensions, wires, etc.) and ferrite core (dimensions, number, arrangement, etc.). Several WPT3 (11.1kVA) solenoid receiver (SR) designs were presented and analyzed based on efficiency, weight, size, volume, and cost. The performance of the proposed SR was compared with the global Double-D receiver (DDR) presented by the SAE J2954 standard under different lateral and rotational alignment and loading conditions considering coupling factor, transmission power and efficiency, and stray electromagnetic fields (EMFs). The entire IPT system including coils, compensation network, power converters, controls, and battery load was modeled and analyzed for both SR and DDR coils. The results reveal that the proposed SR is compatible with the global DD transmitter at different alignment and loading conditions and is able to transmit the desired power (11 kW) at an efficiency > 85%. Hollow SR design shows the highest efficiency and lowest size, weight, and cost compared to DDR and other designs.
Gut microbes predominantly act as living beneficial partners rather than raw nutrients
Authors: da Silva Soares Nuno Filipe , Quagliariello Andrea , Yigitturk Seren , Martino Maria Elena 
Published: July 2023

AbstractAnimals and their gut microbes mutually benefit their health. Nutrition plays a central role in this, directly influencing both host and microbial fitness and the nature of their interactions. This makes nutritional symbioses a complex and dynamic tri-system of diet-microbiota-host. Despite recent discoveries on this field, full control over the interplay among these partners is challenging and hinders the resolution of fundamental questions, such as how to parse the gut microbes’ effect as raw nutrition or as symbiotic partners? To tackle this, we made use of the well-characterized Drosophila melanogaster/Lactiplantibacillus plantarum experimental model of nutritional symbiosis to generate a quantitative framework of gut microbes’ effect on the host. By coupling experimental assays and Random Forest analysis, we show that the beneficial effect of L. plantarum strains primarily results from the active relationship as symbionts rather than raw nutrients, regardless of the bacterial strain. Metabolomic analysis of both active and inactive bacterial cells further demonstrated the crucial role of the production of beneficial bacterial metabolites, such as N-acetylated-amino-acids, as result of active bacterial growth and function. Altogether, our results provide a ranking and quantification of the main bacterial features contributing to sustain animal growth. We demonstrate that bacterial activity is the predominant and necessary variable involved in bacteria-mediated benefit, followed by strain-specific properties and the nutritional potential of the bacterial cells. This contributes to elucidate the role of beneficial bacteria and probiotics, creating a broad quantitative framework for host-gut microbiome that can be expanded to other model systems.
Dynamical system of a time-delayed ϕ6-Van der Pol oscillator: a non-perturbative approach
Authors: Moatimid Galal M. , Amer T. S. 
Published: July 2023

AbstractA remarkable example of how to quantitatively explain the nonlinear performance of many phenomena in physics and engineering is the Van der Pol oscillator. Therefore, the current paper examines the stability analysis of the dynamics of ϕ6-Van der Pol oscillator (PHI6) exposed to exterior excitation in light of its motivated applications in science and engineering. The emphasis in many examinations has shifted to time-delayed technology, yet the topic of this study is still quite significant. A non-perturbative technique is employed to obtain some improvement and preparation for the system under examination. This new methodology yields an equivalent linear differential equation to the exciting nonlinear one. Applying a numerical approach, the analytical solution is validated by this approach. This novel approach seems to be impressive and promising and can be employed in various classes of nonlinear dynamical systems. In various graphs, the time histories of the obtained results, their varied zones of stability, and their polar representations are shown for a range of natural frequencies and other influencing factor values. Concerning the approximate solution, in the case of the presence/absence of time delay, the numerical approach shows excellent accuracy. It is found that as damping and natural frequency parameters increase, the solution approaches stability more quickly. Additionally, the phase plane is more positively impacted by the initial amplitude, external force, damping, and natural frequency characteristics than the other parameters. To demonstrate how the initial amplitude, natural frequency, and cubic nonlinear factors directly affect the periodicity of the resulting solution, many polar forms of the corresponding equation have been displayed. Furthermore, the stable configuration of the analogous equation is shown in the absence of the stimulated force.
Analysis of widefield choroidal thickness maps of healthy eyes using swept source optical coherence tomography
Authors: Hirano Masatoshi , Muraoka Yuki , Kogo Takahiro , Ishikura Masaharu , Nishigori Naomi , Ueda-Arakawa Naoko , Miyata Manabu , Hata Masayuki , Takahashi Ayako , Miyake Masahiro , Tsujikawa Akitaka 
Published: July 2023

AbstractWe aimed to obtain widefield (WF) swept source optical coherence tomography (SS-OCT) data and examine the features of choroidal thickness maps in healthy eyes. The posterior pole choroidal thickness was examined in 127 eyes using enhanced depth imaging of SS-OCT with a viewing angle of 20 (vertical) × 23 (horizontal) mm, and choroidal thickness maps were generated. For SS-OCT image analysis, we developed a grid with inner and outer rings, each divided into superotemporal, inferotemporal, superonasal, and inferonasal quadrants, comprising a total of nine subfields, including the central 3-mm ring. The posterior pole choroidal thicknesses were significantly lower at the periphery than in the central area, in the inferior field than in the superior field, and in the nasal field than in the temporal field (p < 0.001 for all). We also evaluated the effects of age and axial length (AL) on the WF choroidal thickness. The choroidal thickness in all subfields was negatively associated with advanced age (p < 0.05). The choroidal thicknesses in the central and inferonasal inner and outer subfields were negatively associated with AL (p = 0.042, 0.034, and 0.022, respectively). These findings provide insights into the two-dimensional characteristics of choroidal thickness and its association with age and AL.
Static and dynamic adsorption of a gemini surfactant on a carbonate rock in the presence of low salinity water
Authors: Kalam Shams , Abu-Khamsin Sidqi A. , Gbadamosi Afeez Olayinka , Patil Shirish , Kamal Muhammad Shahzad , Hussain Syed Muhammad Shakil , Al-Shehri Dhafer , Al-Shalabi Emad W. , Mohanty Kishore K. 
Published: July 2023

AbstractIn chemical enhanced oil recovery (cEOR) techniques, surfactants are extensively used for enhancing oil recovery by reducing interfacial tension and/or modifying wettability. However, the effectiveness and economic feasibility of the cEOR process are compromised due to the adsorption of surfactants on rock surfaces. Therefore, surfactant adsorption must be reduced to make the cEOR process efficient and economical. Herein, the synergic application of low salinity water and a cationic gemini surfactant was investigated in a carbonate rock. Firstly, the interfacial tension (IFT) of the oil-brine interface with surfactant at various temperatures was measured. Subsequently, the rock wettability was determined under high-pressure and high-temperature conditions. Finally, the study examined the impact of low salinity water on the adsorption of the cationic gemini surfactant, both statically and dynamically. The results showed that the low salinity water condition does not cause a significant impact on the IFT reduction and wettability alteration as compared to the high salinity water conditions. However, the low salinity water condition reduced the surfactant’s static adsorption on the carbonate core by four folds as compared to seawater. The core flood results showed a significantly lower amount of dynamic adsorption (0.11 mg/g-rock) using low salinity water conditions. Employing such a method aids industrialists and researchers in developing a cost-effective and efficient cEOR process.
ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore
Authors: Stevanović Katarina S. , Čepkenović Bogdana , Križak Strahinja , Pajić Tanja , Todorović Nataša V. , Živić Miroslav Ž. 
Published: July 2023

AbstractIon channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability of the apical region of growing sporangiophores of Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets (CDs), to examine ion currents in the filamentous fungi native plasma membrane. In hypoosmotic conditions, the dominant current in the CDs is ORIC, an osmotically activated, anionic, outwardly rectified, fast inactivating instantaneous current that we have previously characterized. Here, we examined the effect of ATP on ORIC. We show that CDs contain active mitochondria, and that respiration inhibition by azide accelerates ORIC inactivation. ATP, added intracellularly, reduced ORIC run-down and shifted the voltage dependence of inactivation toward depolarized potentials, in a manner that did not require hydrolysis. Notably, ATP led to slowing down of ORIC inactivation, as evidenced by an increased time constant of inactivation, τin, and slower decline of τin during prolonged recordings. Flavonoids (genistein and quercetin) had the effect on ORIC opposite to ATP, acting as current inhibitors, possibly by disrupting the stabilizing effect of ATP on ORIC. The integration of osmotic sensing with ATP dependence of the anionic current, typical of vertebrate cells, is described here for the first time in filamentous fungi.
Developing a dual VEGF/PDL1 inhibitor based on high-affinity scFv heterodimers as an anti-cancer therapeutic strategy
Authors: Tzuri Noam , Yegodayev Ksenia M. , Novoplansky Ofra , Elkabets Moshe , Aharoni Amir , Papo Niv 
Published: July 2023

AbstractCancer progression is enhanced by the interaction of programmed death-ligand 1 (PDL1), which is associated with inhibition of the immune response against tumors, and vascular endothelial growth factor (VEGF), which inhibits immune cell activity while inducing angiogenesis and proliferation of cancer cells. Dual inhibition of PDL1 and VEGF may therefore confer a synergistic anti-cancer therapeutic effect. We present a novel strategy for developing a therapeutic that simultaneously binds and inhibits both PDL1 and VEGF. We generated a bi-specific protein, designated DuRan-Bis, comprising a single chain variable fragment (scFv)-based inhibitor of PDL1 fused to an scFv-based inhibitor of VEGF, with the latter being attached to an Fc fragment. We found that DuRan-Bis binds to both PDL1 and VEGF with high affinity. Compared to treatments with mono-specific proteins, alone or in combination, the DuRan-Bis chimera showed superior inhibition of the proliferation of glioblastoma cells. In comparison to treatment with immune cells alone, a combination of immune cells with DuRan-Bis decreased the viability of head and neck cancer cells. To the best of our knowledge, this study is the first to use a single polypeptide chain scFv-scFv-Fc scaffold for engineering a high-affinity bi-specific inhibitor of PDL1 and VEGF.
Assessment of gut microbial β-glucuronidase and β-glucosidase activity in women with polycystic ovary syndrome
Authors: Patel Jalpa , Chaudhary Hiral , Rajput Kiransinh , Parekh Bhavin , Joshi Rushikesh 
Published: July 2023

AbstractPCOS is a prevalent endocrine disorder among women of reproductive age, characterized by hormonal imbalances and metabolic disturbances. This study explores the correlation between gut microbial β-glucuronidase and β-glucosidase and PCOS, focusing on their association with hormone levels and other clinical parameters. In this case-control study, fecal samples were collected from women of reproductive age, both with and without PCOS. The analysis of gut β-glucuronidase and β-glucosidase enzymes was conducted with the other clinical parameters, including body mass index, hormone levels, and hirsutism. These factors were then subjected to correlation analysis. PCOS women showed significantly higher levels of β-glucuronidase activity with a statistically significant P-value (0.05 ± 0.1 vs. 0.04 ± 0.1; P = 0.006) as well as β-glucosidase activity (0.13 ± 0.08 vs. 0.09 ± 0.05; P = 0.06) compared to the controls. This study also revealed intriguing connections between the selected enzymes and hormone levels, particularly testosterone and estradiol. Gut microbial enzymes β-glucuronidase and β-glucosidase may be used as biomarkers for early detection and monitoring of PCOS in women with metabolic challenges. It could lead to improved diagnostic tools and treatment options.
Duality in response of intracranial vessels to nitroglycerin revealed in rats by imaging photoplethysmography
Authors: Sokolov Alexey Y. , Volynsky Maxim A. , Potapenko Anastasiia V. , Iurkova Polina M. , Zaytsev Valeriy V. , Nippolainen Ervin , Kamshilin Alexei A. 
Published: July 2023

AbstractAmong numerous approaches to the study of migraine, the nitroglycerin (NTG) model occupies a prominent place, but there is relatively insufficient information about how NTG affects intracranial vessels. In this study we aim to assess the effects of NTG on blood-flow parameters in meningeal vessels measured by imaging photoplethysmography (iPPG) in animal experiments. An amplitude of the pulsatile component (APC) of iPPG waveform was assessed before and within 2.5 h after the NTG administration in saline (n = 13) or sumatriptan (n = 12) pretreatment anesthetized rats in conditions of a closed cranial window. In animals of both groups, NTG caused a steady decrease in blood pressure. In 7 rats of the saline group, NTG resulted in progressive increase in APC, whereas decrease in APC was observed in other 6 rats. In all animals in the sumatriptan group, NTG administration was accompanied exclusively by an increase in APC. Diametrically opposite changes in APC due to NTG indicate a dual effect of this drug on meningeal vasomotor activity. Sumatriptan acts as a synergist of the NTG vasodilating action. The results we obtained contribute to understanding the interaction of vasoactive drugs in the study of the headache pathophysiology and methods of its therapy.
A performance evaluation of drug response prediction models for individual drugs
Authors: Park Aron , Lee Yeeun , Nam Seungyoon 
Published: July 2023

AbstractDrug response prediction is important to establish personalized medicine for cancer therapy. Model construction for predicting drug response (i.e., cell viability half-maximal inhibitory concentration [IC50]) of an individual drug by inputting pharmacogenomics in disease models remains critical. Machine learning (ML) has been predominantly applied for prediction, despite the advent of deep learning (DL). Moreover, whether DL or traditional ML models are superior for predicting cell viability IC50shas to be established. Herein, we constructed ML and DL drug response prediction models for 24 individual drugs and compared the performance of the models by employing gene expression and mutation profiles of cancer cell lines as input. We observed no significant difference in drug response prediction performance between DL and ML models for 24 drugs [root mean squared error (RMSE) ranging from 0.284 to 3.563 for DL and from 0.274 to 2.697 for ML; R2ranging from −7.405 to 0.331 for DL and from −8.113 to 0.470 for ML]. Among the 24 individual drugs, the ridge model of panobinostat exhibited the best performance (R20.470 and RMSE 0.623). Thus, we selected the ridge model of panobinostat for further application of explainable artificial intelligence (XAI). Using XAI, we further identified important genomic features for panobinostat response prediction in the ridge model, suggesting the genomic features of 22 genes. Based on our findings, results for an individual drug employing both DL and ML models were comparable. Our study confirms the applicability of drug response prediction models for individual drugs.
Myocardial strain-curve deformation patterns after Fontan operation
Authors: Schäfer Michal , Mitchell Max B. , Frank Benjamin S. , Barker Alex J. , Stone Matthew L. , Jaggers James , von Alvensleben Johannes C. , Hunter Kendall S. , Friesen Richard M. , Ivy D. Dunbar , Jacobsen Roni , Di Maria Michael V. 
Published: July 2023

AbstractMyocardial deformation analysis by cardiac MRI (CMR) yielding global circumferential and longitudinal strain (GCS and GLS) is an increasingly utilized method to accurately quantify systolic function and predict clinical events in patients with Fontan circulation. The purpose of this study was to use principal component analysis (PCA) to investigate myocardial temporal deformation patterns derived from strain–time curves to learn about latent strain features beyond peak values. We conducted the study with specific attention to dominant single left or right ventricle (SLV and SRV) morphologies. Methods and Results: Patients remote from Fontan operation who underwent follow-up CMR were analyzed for standard volumetric and function hemodynamics including myocardial deformation parameters including GCS and GLS. We applied PCA to investigate in an unbiased fashion the strain–time curve morphology and to calculate patient specific shape scores. All variables were subjected to single variable Cox regression analysis to detect composite clinical outcome including death, heart transplant, protein losing enteropathy and plastic bronchitis. A total of 122 patients, (SLV = 67, SRV = 55) with a mean age of 12.7 years underwent comprehensive CMR analysis. The PCA revealed 3 primary modes of strain-curve variation regardless of single ventricle morphology and type of strain investigated. Principle components (PCs) described changes in (1) strain–time curve amplitude, (2) time-to-peak strain, and (3) post-systolic slope of the strain–time curve. Considering only SLV patients, GCS was only CMR variable predictive of clinical events (HR 1.46, p = 0.020). In the SRV group, significant CMR predictors of clinical events were derived indexed end-diastolic (HR 1.02, p = 0.023) and end-systolic (HR 1.03, p = 0.022) volumes, GCS (HR 1.91, p = 0.003) and its related first component score (HR 1.20, p = 0.005), GLS (HR 1.32, p = 0.029) and its third component score (HR 1.58, p = 0.017). CMR derived global strain measures are sensitive markers of clinical outcomes in patients with Fontan circulation, particularly in patients with the SRV morphology. Myocardial strain–time curve morphology specific to SLV and SRV patients inspired by unbiased PCA technique can further aid with predicting clinical outcomes.
Bioarchaeological and paleogenomic profiling of the unusual Neolithic burial from Grotta di Pietra Sant’Angelo (Calabria, Italy)
Authors: Fontani Francesco , Boano Rosa , Cinti Alessandra , Demarchi Beatrice , Sandron Sarah , Rampelli Simone , Candela Marco , Traversari Mirko , Latorre Adriana , Iacovera Rocco , Abondio Paolo , Sarno Stefania , Mackie Meaghan , Collins Matthew , Radini Anita , Milani Chantal , Petrella Enrico , Giampalma Emanuela , Minelli Antonella , Larocca Felice , Cilli Elisabetta , Luiselli Donata 
Published: July 2023

AbstractThe Neolithic burial of Grotta di Pietra Sant’Angelo (CS) represents a unique archaeological finding for the prehistory of Southern Italy. The unusual placement of the inhumation at a rather high altitude and far from inhabited areas, the lack of funerary equipment and the prone deposition of the body find limited similarities in coeval Italian sites. These elements have prompted wider questions on mortuary customs during the prehistory of Southern Italy. This atypical case requires an interdisciplinary approach aimed to build an integrated bioarchaeological profile of the individual. The paleopathological investigation of the skeletal remains revealed the presence of numerous markers that could be associated with craft activities, suggesting possible interpretations of the individual’s lifestyle. CT analyses, carried out on the maxillary bones, showed the presence of a peculiar type of dental wear, but also a good density of the bone matrix. Biomolecular and micromorphological analyses of dental calculus highlight the presence of a rich Neolithic-like oral microbiome, the composition of which is consistent with the presence pathologies. Finally, paleogenomic data obtained from the individual were compared with ancient and modern Mediterranean populations, including unpublished high-resolution genome-wide data for 20 modern inhabitants of the nearby village of San Lorenzo Bellizzi, which provided interesting insights into the biodemographic landscape of the Neolithic in Southern Italy.