bluuebunny/crossref_metadata_2025_split huggingface dataset:
P-519 Is oral dydrogesterone a good alternative to vaginal micronized progesterone for luteal phase support in women receiving oocyte donation?
Authors: Jonard S , Lorillon M , Robin G , Keller L 
Published: June 2023

Abstract

Study question
Does oral dydrogesterone (OD) in luteal phase support (LPS) provide equivalent clinical pregnancy and miscarriage rates as micronized vaginal progesterone (MVP) in oocyte donation recipients?

Summary answer
No significant difference in clinical pregnancy and miscarriage rates was observed when using OD instead of MVP for LPS in oocyte donation.

What is known already
Dydrogesterone has a higher bioavailability than MVP due to its high specificity for progesterone receptors allowing the use of lower doses limiting side effects. In addition, because of its route of administration, its tolerance is better. The use of OD for LPS in fresh IVF cycles is now well recognized. However, few data are available on its use in frozen-thawed embryo transfer especially in artificial cycles. In this situation, the lack of corpus luteum leads to a total dependence on exogenous progestogens for implantation and maintenance of the pregnancy.

Study design, size, duration
A retrospective observational study was performed from prospectively collected data in the ART Department of Lille University Hospital from July 2018 to July 2022.

Participants/materials, setting, methods
Our study analysed 372 oocyte donation cycles with embryo transfer. Recipients underwent endometrial preparation by hormone replacement treatment (artificial cycles). LPS was provided by weekly intramuscular progesterone (500 mg/2ml) and either OD 40 mg per day or MVP 800 mg per day for 12 weeks if the pregnancy test was positive. The primary endpoint was clinical pregnancy rate.

Main results and the role of chance
This study compared the outcome of 162 transfers with dydrogesterone + IM progesterone to the outcome of 210 transfers with the MVP + IM progesterone. No significant difference was found between the two groups except for the number of embryos transferred, embryo quality, donor BMI and fresh or frozen status of the embryo. After adjustment for these criteria, our two groups were comparable in clinical pregnancy rates with 36.67% in the MVP group versus 30.25% in the OD group (OR 0.868 [0.529 ; 1.423], p = 0.57), ongoing pregnancy rates (29.05% versus 25.31%, OR 0.993 [0.591; 1.669], p = 0.97), miscarriage rates (7.62% versus 4.94%, OR 0.640 [0.248; 1.650], p = 0.35) and live birth rates (26.67% versus 17.69%, OR 0.727 [0.412; 1.284], p = 0.27).

Limitations, reasons for caution
The main limitation of this study is the non-randomisation of data between the two treatment groups.

Wider implications of the findings
Our study suggests that dydrogesterone has a place in our LPS strategies in artificial cycle for frozen embryo transfers or in oocyte donation, especially as this route is often preferred by patients and very well tolerated. Further prospective cohorts or randomized trials could continue to inform clinical practice on LPS.

Trial registration number
DEC 16-25
P-520 Risk factors for early pregnancy loss in women undergoing in vitro fertilization-embryo transfer
Authors: He Q , Li X , Ouyang Y 
Published: June 2023

Abstract

Study question
What are the risk factors for early pregnancy loss (EPL) in infertility patients undergoing in vitro fertilization-embryo transfer (IVF-ET) cycles?

Summary answer
The risk factors for EPL were maternal age, 14-day β-human chorionic gonadotrophin(β-hcg) levels and cycle type.

What is known already
Pregnancy obtained through assisted reproductive technology still has a high risk of spontaneous early pregnancy abortion, and the cause of which is unclear. It not only affects the rate of ongoing pregnancy and live birth, but also increases the psychological and economic burden of patients. At present, there have been many studies on the risk factors of early pregnancy loss in IVF/ICSI-ET at domestic and overseas, but the results are different, which may be related to the selection of different research objects, sample size and statistical methods.

Study design, size, duration
A retrospective study of 6107 embryo transfer cycles collected from January 2018 to December 2018 was performed. The cycles were divided into the EPL group (n = 987) and the live birth group (n = 5120) according to the cycle outcomes.

Participants/materials, setting, methods
Baseline data and clinically relevant indicators were compared between the two groups, including maternal age, infertility years, infertility type, body mass index (BMI), tubal factor, ovulatory dysfunction, endometriosis, male factor, antral follicle count (AFC), single gene related genetic disease, follicle-stimulating hormone (FSH), estradiol (E2), luteinizing hormone (LH), 14-day β-hcg levels, prolactin (PRL), progesterone (P), testosterone (T), anti-Müllerian hormone (AMH), cycle type and the number of transplanted embryos.

Main results and the role of chance
The EPL rate of the infertility patients undergoing fresh/frozen-thaw embryo transfer cycle was 16.16%. Univariate analysis showed that there were statistically significant differences between the two groups in maternal age, AFC, 14-day β-hcg levels, T, AMH, infertility years, cycle type, infertility types, chromosome disease and male factors (P &lt; 0.05). The results of binary logistics regression analysis showed that maternal age, 14-day β-hcg levels and cycle type were related factors to EPL (P &lt; 0.05).

Limitations, reasons for caution
This study lacked some baseline data, such as adverse pregnancy history, abortion history, and previous transplant failure history, and male factors such as BMI, age, semen results, smoking history. The information about fetal chromosomes was also missing.

Wider implications of the findings
Clinical prevention and treatment of risk factors can be targeted to improve pregnancy outcomes after transplantation.

Trial registration number
none
P-599 The use of serum test to detect the LH surge in ultrasound-monitored intrauterine insemination (IUI) significantly increases pregnancy rates.
Authors: Kim M J , Kim S G , Kim H Y , Park J S , Son H J , Kim G W , Heo G D , Seong J Y , Noh H K , Lee K H 
Published: June 2023

Abstract

Study question
Could a serum test to detect LH surge in ultrasound-monitored IUI cycle improve pregnancy rates?

Summary answer
Serum test to detect LH surges with ultrasound monitoring during IUI cycle significantly increased pregnancy rates.

What is known already
IUI is known as the first line of treatment for infertile couples and is usually administered with human chorionic gonadotropin (hCG) before ovulation with clomiphene citrate (CC) or/and human menopausal gonadotropin (hMG) to ovarian known to stimulate. One of the key factor for IUI is that insemination should be performed close to ovulation time, as there are many reports that sperm injection at the ovulation time has a high pregnancy rates. However, there are still few reports on the optimal timing of ovulation for performing IUI.

Study design, size, duration
The IUI performed from January 2016 through November 2022 (n = 3187) was included in this study. A total of 875 patients underwent the IUI by inferring LH surge with procedure with serum test, while 2312 patients underwent the procedure IUI without the serum test (control).

Participants/materials, setting, methods
In this study included 3187 IUI cycle were analyzed. Controlled ovarian hyperstimulation was conducted with CC (100 mg/day) and hMG. Patients were divided into two groups with serum test or not test (control). The serum test group was monitored LH surge by inferring LH peak during IUI cycle. When the sufficient follicular size and endometrial thickness had been reached, hCG was administrated and insemination was performed. Pregnancy rates were compared by Chi-squared test.

Main results and the role of chance
In each group, there were no significantly difference in age (34.4±3.7 vs. 34.7±3.7 years old) and endometrial thickness (8.8±2.2 vs. 8.9±2.2 mm) for IUI cycle condition. The serum test group was significantly higher clinical pregnancy rates compared to control group (19.0 vs. 15.7 % respectively) during IUI cycle (P &lt; 0.05). In addition, patients were classified according to the history of at least one or more failed IUI cycle. The clinical pregnancy rates was significantly higher in the serum test group than control group (21.6 vs. 10.8 % respectively) who history of at least one or more failed IUI cycle (P &lt; 0.001).

Limitations, reasons for caution
A limitation of this study has a too small size of the intervention group compared with the control group.

Wider implications of the findings
In our results, the serum test group had a significantly higher clinical pregnancy rates than the control group. Therefore, detecting a surge in LH with a serum test during the IUI cycle could predict the optimal timing of ovulation and thus increase the pregnancy rates.

Trial registration number
not applicable
P-672 Vaginal microbiome changes in women with polycystic ovary syndrome: a large cross-sectional study
Authors: Zhao S , Jin C , Qin L , Zhao H , Chen Z J 
Published: June 2023

Abstract

Study question
How the vaginal microbiome changes in women with polycystic ovary syndrome?

Summary answer
The PCOS women had a higher diversity in the vaginal microbiome and showed an enhanced level of heterogeneity.

What is known already
PCOS is a common reproductive endocrine disorder with a highly heterogeneous clinical presentation. Recently, few studies with limited sample size have reported that the inconsistent vaginal microbiota composition changes in PCOS.

Study design, size, duration
The cross-sectional study conducted at a single academic university-affiliated center. A total of 1,446 subjects, including 713 PCOS cases and 733 controls, were recruited.

Participants/materials, setting, methods
Vaginal swabs were collected for subsequent 16S rRNA gene sequencing. The microbiome diversities, community distances, vaginal bacterial relative abundances, and microbial interactions network were compared between the PCOS group and control group.

Main results and the role of chance
The PCOS group had a higher alpha diversity in the vaginal microbiome than the control (P &lt; 0.05), while higher intra-group variability was observed in PCOS (P &lt; 0.05). At the genus level, the abundance of Lactobacillus in the PCOS group decreased, while the abundance of Gardnerella and Ureaplasma increased (FDR&lt;0.2). Gardnerella vaginalis, Prevotella buccalis, and Prevotella timonensis were identified as differential species and were significantly associated with clinical parameters of PCOS, like AMH. The microbial interaction network analysis revealed that Prevotella and Lactobacillus could be key drivers for network rewiring in PCOS. Notable alterations of predicted pathways, which significantly differed between PCOS and control women, mainly enriched in amino acid metabolism.

Limitations, reasons for caution
The study only included Chinese Han population data. Due to the structure of the vaginal flora may vary between ethnic groups, generalizing the results of this study to other populations should be taken caution.

Wider implications of the findings
This study with large samples could enhance our understanding of the PCOS vaginal microbiome, and provide a basis for future research on the potential mechanism by which pathogenic bacteria are involved in PCOS vaginal microbial imbalances.

Trial registration number
Not applicable
Evaluating imaging uncertainty associated with the near surface and added value of vertical arrays using Bayesian seismic refraction tomography
Authors: Silvestrov Ilya , Egorov Anton , Bakulin Andrey 
Published: June 2023

AbstractTraditional tomographic methods do not consider the uncertainties associated with near-surface velocities and static corrections and provide a deterministic solution to the estimation problem. However, these uncertainties significantly affect structural mapping and interpretation of seismic imaging results. On the other hand, Bayesian first-arrival tomography provides multiple near-surface models that fit observed traveltimes equally well and enable the study of potential solution distributions. We demonstrate this approach on a complex synthetic near-surface model, representative of arid environments, to quantify associated velocity and statics uncertainties. We evaluate two different parameterizations for subsurface velocities in the context of near-surface Bayesian tomography: Voronoi tessellation with natural neighbor interpolation and the more conventional Delaunay triangulation with linear interpolation. Our analysis shows that the Voronoi cell parameterization with natural neighbor interpolation is more appropriate for this problem. Finally, the new approach is applied to compare two alternative acquisition geometries comprising conventional surface receivers and surface receivers augmented with vertical receiver arrays. The results demonstrate that adding vertical receiver arrays to conventional surface receivers can significantly reduce the near-surface velocity uncertainty and thus increases the accuracy of the seismic imaging results. Furthermore, the study shows that Bayesian tomography can be used as a tool for evaluating different source and receiver geometries during the acquisition design stage.
Massive dermal ulcerative lesions because of brown recluse spider bite: a rare case report and review of literature
Authors: Fakhar Mahdi , Alian Shahriar , Zakariaei Ashkan , Nourzad Fatemeh , Zakariaei Zakaria 
Published: June 2023

Abstract
A brown recluse spider (BRS) bite is challenging to confirm, but can be clinically diagnosed by considering the location, the season of the year and the clinical manifestations. We described a 26-year-old male who presented after a BRS bite with a skin lesion, bruising, severe swelling and diffuse blisters on the right lower extremity after 3 days. This case should be considered in the differential diagnosis of necrotizing fasciitis. Although spider bite poisoning is rare, proper diagnosis and management are important because, in some cases, it can have devastating outcomes.
Mineralogy and Geochemistry of Nepheline Syenite From the Bang Phuc Massif of the Alkaline Cho Don Complex in North-Eastern Vietnam—Implications for Magma Evolution and Fluid–Rock Interactions
Authors: Dumańska-Słowik M , Powolny T , Nguyen Khac G 
Published: June 2023

Abstract
The alkaline Cho Don complex in NE Vietnam comprises several mafic-felsic suites related to the widespread magmatism developed during the early Permian–late Triassic. The contribution explores the petrogenesis of nepheline syenite from the Bang Phuc massif and its petrogenetic relationship with cogenetic scapolite-rich gabbro. The nepheline syenite formed through fractional crystallization of pristine mantle-derived basaltic melt modified by subduction-related components (chiefly sediment-derived melts), as shown by, e.g. low Ba/Th and high Th/Nb ratios of the rocks. The transition from gabbro to syenite follows a within-plate enrichment trend (e.g. increasing Ta/Yb, Nb/Yb, and Th/Yb ratios) that might reflect switch from post-orogenic to intra-plate regimes, accompanied by subduction–collision–extension events related to the Indosinian Orogeny. Furthermore, magma evolution involved the progressive contribution of asthenospheric-derived melts that resulted in the appearance of OIB-like signatures (e.g. high Nb/La ratios) in the nepheline syenite. Fractional crystallization of fluorapatite and mafic phases, as well as assimilation of carbonate wall rocks ultimately led to the decrease of LREE contents and/or modification of Zr/Hf ratios. Magmatic phases of the nepheline syenite include nepheline, sodalite, oligoclase, orthoclase, and annite, as well as accessory fluorapatite, fluorite, and minor amounts of zircon and metamict allanite-Ce. The nepheline equilibrated at temperatures ranging between 850°C and 700°C, which reflects protracted residence at a higher temperature. Later, it has been locally altered to cancrinite, dawsonite, and natrolite via CO2- and alkali-rich fluid influx. The fluid–rock interactions were also manifested by the presence of chessboard-twinned albite and coarsening of braid-perthite into patch-perthite, as well as recrystallization of primary orthoclase into microcline. The orthoclase→microcline conversion, albeit fairly indiscrete under a polarizing microscope and confirmed by Raman micro-spectroscopy, is followed by the change of cathodoluminescence colours, i.e. from light-blue (activated by Ti4+ and/or Al-O—Al centres) in orthoclase towards brownish and/or greenish (activated by Mn2+ and structural defects) in microcline.
Marketing the Self vs. Preserving the Self: Resisting Downward Mobility in the New Economy
Authors: Lopez Steven , Ibañez Lindsey 
Published: June 2023

AbstractHow do dislocated workers try to avoid downward mobility as they navigate insecure, nonstandard, and precarious work in the contemporary American economy? Should they embrace flexibility, or follow their passions? Drawing on in-depth, qualitative interviews with 56 displaced job seekers, we extend the job searching literature to distinguish two kinds of job searching: self-marketing and self-preservation. Self-marketers are willing to reinvent themselves to pursue opportunities wherever they perceive the best payoffs. By contrast, self-preservers, drawing on affective commitments to particular kinds of work, limit their searches to their current occupation. In this paper, we show how the neoliberal economy buffets and preys on both types of job seekers and how bounded rationality and asymmetric information problems leave self-marketers vulnerable to downward mobility via cons, scams, and predatory business models. But self-preservation searches contain their own pathways to downward mobility: descents into low-wage work that begin as temporary measures often become permanent. Thus, even though self-marketers and self-preservers embrace very different job search strategies, neither flexibility nor passion offers protection against downward mobility in the post-Great Recession economy. We conclude with some reflections on how these two job search orientations may help us better understand current labor market upheavals.
Revision of the generic classification of pike cichlids using an integrative phylogenetic approach (Cichlidae: tribe Geophagini: subtribe Crenicichlina)
Authors: Varella Henrique R , Kullander Sven O , Menezes Naércio A , Oliveira Claudio , López-Fernández Hernán 
Published: June 2023

AbstractPike cichlids form the largest clade of Neotropical cichlids, with over 100 species presently classified in two genera: Crenicichla (93 species widespread in rivers of South America east of the Andes) and Teleocichla (nine rheophilic Amazonian species). Here, we combined a new dataset of 216 morphological characters with molecular data compiled from published sources, comprising 74 terminal taxa of pike cichlids (68 out of 102 valid species, plus four putative new species), and performed phylogenetic analyses using maximum likelihood, Bayesian inference, and parsimony. Based on a synthesis of our results and previous phylogenies, we propose a new classification in which the clade including all pike cichlids is elevated to the rank of subtribe (Crenicichlina) and the genus Crenicichla is redefined, including three subgenera: Crenicichla (monotypic with the type species), Batrachops (resurected as subgenus), and Lacustria (new subgenus). Teleocichla is maintained as a valid genus and four new genera are proposed: Wallaciia, Saxatilia, Hemeraia, and Lugubria. Our results on character mapping support the hypothesis that resource partitioning in environments with fast-flowing water and rocky beds might have played a role in the origin or maintenance of the great diversity of pike cichlids, resulting in parallel evolution of similar ecomorphs.
The Ralstonia Research Community Rejects the Proposal to Classify Phylotype I Ralstonia into the New Species Ralstonia nicotianae
Authors: Lowe-Power Tiffany , Sharma Parul , Alfenas-Zerbini Poliane , Álvarez Belén , Arif Mohammad , Baroukh Caroline , Bocsanczy Ana Maria , Biosca Elena G. , Castillo José A. , Cellier Gilles , Coutinho Teresa , Drenth André , Friman Ville-Petri , Genin Stephane , Guidot Alice , Hikichi Yasufumi , Huang Qi , Iyer-Pascuzzi Anjali , Kai Kenji , Pecrix Yann , Poussier Stephane , Ray Jane D. , Rossato Maurício , Schomer Rebecca , Siri Maria Inés , Vinatzer Boris A. , Allen Caitilyn 
Published: June 2023

The Ralstonia solanacearum species complex is a group of globally important plant pathogens. Bacteria in this very large and genetically diverse group all colonize the xylem elements of angiosperm plants and cause high-impact wilting diseases of many crops. Because they threaten economic and food security, several R. solanacearum species complex subgroups are strictly regulated as quarantine pests. Biologically meaningful and consistent nomenclature is essential for organisms that have major economic and regulatory importance, such as plant-pathogenic Ralstonia. There are currently three species of Ralstonia wilt pathogens: R. pseudosolanacearum (corresponding to two phylogenetic groups that are described in the literature as phylotypes I and III), R. solanacearum (phylotypes IIA, IIB, and IIC), and R. syzygii (phylotype IV, containing three subspecies: subsp. syzygii, subsp. celebensis, and subsp. indonesiensis). A recent paper proposed reclassifying phylotype I as a new species named “ Ralstonia nicotianae.” The purpose of this commentary is to register our objection to the taxon “ Ralstonia nicotianae.” [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
Advanced methodology for bacterial colonization of 3D organotypic epidermal models: a gateway to long-term host-microbe interaction and intervention studies
Authors: Rikken Gijs , Meesters Luca D. , Jansen Patrick A.M. , Rodijk-Olthuis Diana , van Vlijmen-Willems Ivonne M.J.J. , Niehues Hanna , Oláh Peter , Homey Bernhard , Schalkwijk Joost , Zeeuwen Patrick L.J.M. , van den Bogaard Ellen H. 
Published: June 2023

ABSTRACTBackgroundFollowing descriptive studies on skin microbiota in health and disease, mechanistic studies on the interplay between skin and microbes are on the rise, for which experimental models are in great demand. Here, we present a novel methodology for microbial colonization of organotypic skin and analysis thereof.ResultsAn inoculation device ensured a standardized application area on thestratum corneumand a homogenous distribution of bacteria, while preventing infection of the basolateral culture medium even during prolonged co-culture periods for up to two weeks at a specific culture temperature and humidity. Hereby, host-microbe interactions and antibiotic interventions could be studied, revealing diverse host responses to various skin-related bacteria and pathogens.ConclusionsOur methodology is easily transferable to a wide variety of organotypic skin or mucosal models and different microbes at every cell culture facility at low costs. We envision that this study will kick-start skin microbiome studies using human organotypic skin cultures, providing a powerful alternative to experimental animal models in pre-clinical research.