bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Intraoperative Rapid Molecular Diagnosis in Precision Surgery for Glioma: Mimic the World Health Organization CNS5 Integrated Diagnosis
Authors: Xue Hao , Han Zhe , Li Haiyan , Li Xueen , Jia Deze , Qi Mei , Zhang Hui , Zhang Kailiang , Gong Jie , Wang Hongwei , Feng Zichao , Ni Shilei , Han Bo , Li Gang 
Published: January 2023

BACKGROUND:
With the advent of the molecular era, the diagnosis and treatment systems of glioma have also changed. A single histological type cannot be used for prognosis grade. Only by combining molecular diagnosis can precision medicine be realized.

OBJECTIVE:
To develop an automatic integrated gene detection system (AIGS) for intraoperative detection in glioma and to explore its positive role in intraoperative diagnosis and treatment.

METHODS:
We analyzed the isocitrate dehydrogenase 1 (IDH1) mutation status of 105 glioma samples and evaluated the product's potential value for diagnosis; 37 glioma samples were detected intraoperatively to evaluate the feasibility of using the product in an actual situation. A blinding method was used to evaluate the effect of the detection technology on the accuracy of intraoperative histopathological diagnosis by pathologists. We also reviewed the current research status in the field of intraoperative molecular diagnosis.

RESULTS:
Compared with next-generation sequencing, the accuracy of AIGS in detecting IDH1 was 100% for 105 samples and 37 intraoperative samples. The blind diagnostic results were compared between the 2 groups, and the molecular information provided by AIGS increased the intraoperative diagnostic accuracy of glioma by 16.2%. Using the technical advantages of multipoint synchronous detection, we determined the tumor molecular margins for 5 IDH-positive patients and achieved accurate resection at the molecular level.

CONCLUSION:
AIGS can quickly and accurately provide molecular information during surgery. This methodology not only improves the accuracy of intraoperative pathological diagnosis but also provides an important molecular basis for determining tumor margins to facilitate precision surgery.
CCDC66 regulates primary cilium length and signaling via interactions with transition zone and axonemal proteins
Authors: Odabasi Ezgi , Conkar Deniz , Deretic Jovana , Batman Umut , Frikstad Kari-Anne M. , Patzke Sebastian , Firat-Karalar Elif Nur 
Published: January 2023

ABSTRACT
The primary cilium is a microtubule-based organelle that serves as a hub for many signaling pathways. It functions as part of the centrosome or cilium complex, which also contains the basal body and the centriolar satellites. Little is known about the mechanisms by which the microtubule-based ciliary axoneme is assembled with a proper length and structure, particularly in terms of the activity of microtubule-associated proteins (MAPs) and the crosstalk between the different compartments of the centrosome or cilium complex. Here, we analyzed CCDC66, a MAP implicated in cilium biogenesis and ciliopathies. Live-cell imaging revealed that CCDC66 compartmentalizes between centrosomes, centriolar satellites, and the ciliary axoneme and tip during cilium biogenesis. CCDC66 depletion in human cells causes defects in cilium assembly, length and morphology. Notably, CCDC66 interacts with the ciliopathy-linked MAPs CEP104 and CSPP1, and regulates axonemal length and Hedgehog pathway activation. Moreover, CCDC66 is required for the basal body recruitment of transition zone proteins and intraflagellar transport B (IFT-B) machinery. Overall, our results establish CCDC66 as a multifaceted regulator of the primary cilium and provide insight into how ciliary MAPs and subcompartments cooperate to ensure assembly of functional cilia.
Influence of surface free energy of the substrate and flooded water on the attachment performance of stick insects (Phasmatodea) with different adhesive surface microstructures
Authors: Thomas Julian , Gorb Stanislav N. , Büscher Thies H. 
Published: January 2023

ABSTRACTStick and leaf insects (Phasmatodea) are exclusively herbivores. As they settle in a broad range of habitats, they need to attach to and walk on a wide variety of plant substrates, which can vary in their surface free energy (SFE). The adhesive microstructures (AMs) on the euplantulae of phasmids are assumed to be adapted to such substrate properties. Moreover, the natural substrates can often be covered with water as a result of high relative humidity or rain. Although considerable experimental research has been carried out on different aspects of stick insect attachment, the adaptations to cope with the influence of flooded water on attachment performance remain unclear. To elucidate the role of AMs in this context, we here measured attachment forces in three species of stick insects with different AMs. The results show that attachment forces of the three species studied were influenced by the SFE and the presence of water: they all showed higher pull-off (vertical) and traction (horizontal) forces on dry surfaces, compared with when the surfaces were covered with a water film. However, the extent to which the surface properties influenced attachment differed depending on the species and its AMs. All three species showed approximately the same attachment performance on dry surfaces with different surface free energy but maintained attachment underwater to different extents.
Silsesquioxane Cage-Fused Siloxane Rings as a Novel Class of Inorganic-Based Host Molecules
Authors: Okamoto Keigo , Imoto Hiroaki , Naka Kensuke 
Published: January 2023

Abstract
Side-opened cage-silsesquioxane, denoted as SO-POSS, was fused with oligo(dimethylsiloxane)s to synthesize inorganic skeleton-based cyclic molecules. The resultant cyclic compounds served as hosts for alkali metal cations (Li+, Na+, K+). Interestingly, ring-transformation occurred in the presence of guest cations depending on the ring size; the SO-POSS-fused cyclic oligo(dimethylsiloxane)s were converted to smaller SO-POSS rings and cyclic oligo(dimethylsiloxane)s. Herein, we report the first study on host-guest chemistry of the cage silsesquioxane-fused siloxane rings.
Photovoltaic Properties of π-Conjugated Polymers Based on Fused Cyclic Imide and Amide Skeletons
Authors: Tsuchii Yuichi , Menda Taiki , Hwang Sunbin , Yasuda Takuma 
Published: January 2023

Abstract
π-Conjugated polymers P(BDT-BTI) and P(BDT-BTA) bearing cyclic imide and amide (lactam) moieties, respectively, were designed and synthesized for application in organic photovoltaics (OPVs). Introducing electron-withdrawing imide and amide moieties lowers the HOMO energy levels of the polymers, resulting in an increase in the open-circuit voltages of OPVs. Complementary photoabsorption over the entire visible range was achieved by combining P(BDT-BTI) and P(BDT-BTA) with a typical non-fullerene acceptor, IT-4F. Unlike P(BDT-BTI), P(BDT-BTA) tends to maintain high crystallinity and retains π–π stacking interactions and face-on orientation in the blend films with IT-4F. Consequently, OPVs based on P(BDT-BTA):IT-4F blends achieved power conversion efficiencies as high as 9.6% without any processing additives or post-treatments.
Prepare for the Unexpected: A New Look at Trauma Triage and Care in Mass Participation Sporting Events
Authors: Sedgley Matt , Hudson Korin , Hulsopple Chad 
Published: January 2023

Abstract
Few sports medicine physicians have extensive experience in managing mass casualty trauma. Yet many, or most, of us find ourselves covering competitions, races, and events in venues where thousands of athletes and spectators gather, and these could quickly become mass casualty events in the setting of an explosion/bombing, active shooter, or similar incident. When this happens, the sports medicine team, already on site, is in a unique position to provide immediate assistance until additional resources arrive. In this article, we will discuss a framework for this initial trauma response to the mass casualty event at a mass participation sporting event.
When Is Air Travel Safe after Mild Traumatic Brain Injury/Sports-Related Concussion?
Authors: Alvarez Bianca E. , McGrew Christopher A. , Bossart Christopher 
Published: January 2023

Abstract
While the literature regarding return to play and recovery protocols from mild traumatic brain injury (mTBI) and sports-related concussions (SRC) is growing, there continues to be a paucity of data regarding when air travel is safe for athletes after sustaining certain brain injuries, such as mTBI and SRC. Although it is known hypoxia can negatively affect severe TBI patients, it is unclear whether mild hypoxia, which may be experienced during commercial air travel, is clinically significant for athletes who have recently sustained mTBI injuries. Further research is required to provide more standardized recommendations on when air travel is safe. With the current available literature, clinicians still need to weigh the evidence, consider how it applies to each individual patient, and engage in shared decision making to ultimately decide what is best for the patient.
Online Sports Medicine Fellowship Education: The Genesis of a National Program and Year-1 Analysis
Authors: Moeller James L. , Zaremski Jason L. , Rao Ashwin , Denq William , Saffel Heather L. , Nye Nathaniel S. , Waterbrook Anna L. 
Published: January 2023

Abstract
The COVID-19 pandemic has created numerous challenges in all walks of life. One such challenge was the strain and subsequent effects on medical education, including the elimination of in-person learning opportunities. Consequently, in March of 2020, a nationwide Sports Medicine fellowship online education series was developed. Presentations were available for live and recorded viewing. Over the course of the 2020–2021 academic year, 38 presentations were offered, covering 45 topics. Live viewership totaled nearly 1600 through the year, while views of recorded lecture reached nearly 34,000. There was no statistical difference in the number of viewers for musculoskeletal versus nonmusculoskeletal topics in either the live (46.50 ± 35.37 vs. 43.38 ± 27.28 viewers, respectively; P = 0.77) or recorded formats (843.60 ± 337.66 vs 876.67 ± 624.70 viewers, respectively; P = 0.85). This article presents the novel approach to sports medicine education by the American Medical Society for Sports Medicine in the 2020–2021 academic year through the genesis the National Online Fellowship Education Program along with analyses of viewership data.
Changes in resting state networks in high school football athletes across a single season
Authors: Ghiles Connor W. , Clark Michael D. , Kuzminski Samuel J. , Fraser Melissa A. , Petrella Jeffrey R. , Guskiewicz Kevin M. 
Published: January 2023

Objective: The aim of this pilot cohort study was to examine changes in the organization of resting-state brain networks in high school football athletes and its relationship to exposure to on-field head impacts over the course of a single season. Methods: Seventeen male high school football players underwent functional magnetic resonance imaging and computerized neurocognitive testing (CNS Vital Signs) before the start of contact practices and again after the conclusion of the season. The players were equipped with helmet accelerometer systems (Head Impact Telemetry System) to record head impacts in practices and games. Graph theory analysis was applied to study intranetwork local efficiency and strength of connectivity within six anatomically defined brain networks. Results: We observed a significant decrease in the local efficiency (−24.9 ± 51.4%, r = 0.7, p < 0.01) and strength (−14.5 ± 26.8%, r = 0.5, p < 0.01) of functional connectivity within the frontal lobe resting-state network and strength within the parietal lobe resting-state network (−7.5 ± 17.3%, r = 0.1, p < 0.01), as well as a concomitant increase in the local efficiency (+55.0 +/- 59.8%, r = 0.5, p < 0.01) and strength (+47.4 +/- 47.3%, r = 0.5, p < 0.01) within the mediotemporal networks. These alterations in network organization were associated with changes in performance on verbal memory (p < 0.05) and executive function (p < 0.05). We did not observe a significant relationship between the frequency or cumulative magnitude of impacts sustained during the season and neurocognitive or imaging outcomes (p > 0.05). Conclusion: Our findings suggest the efficiency and strength of resting-state networks are altered across a season of high school football, but the association of exposure levels to subconcussive impacts is unclear. Advances in knowledge: The efficiency of resting-state networks is dynamic in high school football athletes; such changes may be related to impacts sustained during the season, though further study is needed.
Differences in MRI findings of superficial spindle cell lipoma and atypical lipomatous tumor/well-differentiated liposarcoma
Authors: Kawaguchi Masaya , Kato Hiroki , Kobayashi Kazuhiro , Miyazaki Tatsuhiko , Nagano Akihito , Noda Yoshifumi , Hyodo Fuminori , Matsuo Masayuki 
Published: January 2023

Objective: This study aimed to evaluate the efficacy of using MRI findings to differentiate superficial spindle cell lipomas (SCLs) from atypical lipomatous tumor/well-differentiated liposarcomas (ALT/WDLs). Methods: This study included 12 patients with histopathologically proven superficial SCLs and 11 with ALT/WDLs. MRI findings for both pathologies were retrospectively reviewed and compared between the two pathologies. Results: The neck, upper back, and shoulder regions were more frequent locations of SCLs than of ALT/WDLs (100% vs 55%, p < 0.05), whereas no significant differences were observed in age and sex. The median maximum diameter of the lesion was smaller in SCLs than in ALT/WDLs (44 mm [interquartile range (IQR): 35–63] vs 102 mm [IQR: 86–119], p < 0.05). On T 1 weighted images, non-fatty area was more frequently observed in SCLs than in ALT/WDLs (73% vs 25%, p < 0.05), and the median rate of non-fatty area was larger in SCLs than in ALT/WDLs (7.5% [IQR: 1.0–53] vs 0% [IQR: 0–0.2], p < 0.05). On fat-suppressed T 2 weighted images, a solid hyperintense area was more frequently observed in SCLs than in ALT/WDLs (83% vs 27%, p < 0.05). Conclusion: The maximum diameter, non-fatty area on T 1 weighted images, and solid hyperintense area on fat-suppressed T 2 weighted images were useful imaging features for differentiating superficial SCLs from ALT/WDLs. Advances in knowledge: In superficial lipomatous tumors, small tumor size and non-fatty solid area were valuable findings for diagnosing SCLs.
Stroke prevention – review of the latest reports
Authors: Kasperski Radosław , Piekarska Małgorzata , Pacek Katarzyna , Pikulicka Agata , Szulc Izabela , Jedlina Klaudia , Swacha Weronika , Świercz Kamila 
Published: January 2023

Introduction and purpose: According to WHO 15 million people suffer from stroke, one-third of which dies and the other one-third is permanently disabled. For this reason, this condition is a very important medical and social problem. It is one of the leading causes of death, after cardiovascular diseases and cancer. It is also one of the most common causes of disability in the adult population. The economic aspects are related not only to the costs of hospital treatment, but above all to rehabilitation and chronic treatment. The aim of this article is to present the most important stroke risk factors and, based on them, to describe recommendations for effective stroke prevention.
A brief description of the state of knowledge: In recent years, numerous studies have been conducted on the relationship between various factors and the risk of stroke. It turned out that many stroke risk factors are modifiable and by using various methods we are able to significantly reduce the risk of its occurrence. The most important risk factor is the patient's hypertension. Others are cardiovascular diseases such as atrial fibrillation; diabetes, obesity, low level of physical activity, improper diet, consumption of alcohol and tobacco products.
Summary: Through education and promotion of a healthy lifestyle in society (high physical activity, healthy diet, lack of addictions, taking care of mental health and avoiding excessive stress), as well as regular blood pressure and glucose control and effective treatment of conditions leading to stroke, the incidence of stroke among people can actually be reduced. This issue still requires further research and constant expansion of our knowledge.
Does a One-Size-Fits-All Minimum Wage Cause Financial Stress for Small Businesses?
Authors: Chava Sudheer , Oettl Alexander , Singh Manpreet 
Published: January 2023

Using intertemporal variation in the bounding of a state’s minimum wage by the federal rate and business credit-score data for 15.2 million establishments, we find that the increase in labor costs caused by a higher federal minimum wage leads to lower business credit scores and worsens the financial health of small businesses in the affected states. In particular, small, young, labor-intensive, and minimum-wage-sensitive establishments located in affected states and those located in competitive and low-income areas experience higher financial stress. Increases in the minimum wage are associated with employment reductions and a higher exit rate for small businesses. Our results document some potential costs of a one-size-fits-all nationwide minimum wage for some small businesses. This paper was accepted by Gustavo Manso, finance. Funding: This work was supported by the Ewing Marion Kauffman Foundation Junior Faculty Fellowship. Supplemental Material: The data and internet appendix are available at https://doi.org/10.1287/mnsc.2022.4620 .
The Price of Anarchy for Instantaneous Dynamic Equilibria
Authors: Graf Lukas , Harks Tobias 
Published: January 2023

We consider flows over time within the deterministic queueing model of Vickrey and study the solution concept of instantaneous dynamic equilibrium (IDE), in which flow particles select at every decision point a currently shortest path. The length of such a path is measured by the physical travel time plus the time spent in queues. Although IDE has been studied since the eighties, the worst-case efficiency of the solution concept with respect to the travel time is not well understood. We study the price of anarchy for this model under the makespan and total travel time objective and show the first known upper bound for both measures of order [Formula: see text] for single-sink instances, where U denotes the total inflow volume and τ denotes the sum of edge travel times. We complement this upper bound with a family of quite complex instances proving a lower bound of order [Formula: see text]. Funding: The research of the authors was funded by the Deutsche Forschungsgemeinschaft [Grants HA 8041/1-1 and HA 8041/4-1].
Performance of intensive vannamei (Litopenaeus vannamei) culture at low salinity
Authors: Cahyanurani Annisa Bias , Edy Mochammad Heri 
Published: January 2023

Intensive aquaculture systems that are not managed properly often cause problems that disrupt the balance of the environment so that shrimp are susceptible to disease. One of the aquaculture innovations that have been developed to minimize disease impact is shrimp farming with low salinity. This study aims to determine the performance of intensive Litopenaeus vannamei shrimp culture with low salinity. This study used quantitative descriptive analysis with a purposive sampling technique in three ponds for three maintenance cycles. The result of the study on three cycles of vannamei shrimp culture with low salinity overall found that productivity ranged from 2.35 to 3.69 kg/m2. ADG values in aquaculture ponds ranged from 0.26 – 0.36 g/day. The survival rate ranged from 61% to 98%, and the feed conversion ratio ranged from 1.01 to 1.62. The survival rate tends to increase, and FCR value tends to decrease in 3 cycles of cultivation. Water quality including temperature, dissolved oxygen, pH, phosphate, ammonia, and TOM indicate the optimal range based on water quality standards for intensive vannamei shrimp culture (SNI 01-7246-2006). At the same time, nitrite and alkalinity exceed the SNI standard but this nitrite and alkalinity level could still be tolerated by vannamei shrimp during the rearing period. Overall, water quality during the rearing period can still support the growth of vannamei shrimp. The above result showed that vannamei shrimp culture at low salinity (6-8 mg L-1) has the potential to be developed.Keywords:Intensive culture Litopenaeus vannamei Low salinityProductivity
The effect of aquous extract of johar leaves (Senna siamea) injection on survival rate and blood profile of snakehead fish (Channa striata)
Authors: Sutriana Amalia , Panjaitan Budianto , Siddik Tasya Luthfiah , Farida Farida , Hanafiah Muhammad , Asmilia Nuzul , Rosmaidar Rosmaidar , Rahmad Rahmad 
Published: January 2023

Aqueous extract of johar leaf contains metabolite compounds such as flavonoids, sponins, tannins, phenols, anthraquinones, anthocyanins, and cardiac glycosides which can be used to substitute commercial antibiotics. The use of aqueous extract of johar leaf has been known to have antibacterial activity in vitro. This study aims to determine the effect of aqueous extract of johar leaves (Senna siamea) injection on survival and blood profile of snakehead fish (Channa striata) in vivo. This research was a laboratory study using a completely randomized design (CRD) with 5 treatments and 3 replications with a total sample of 75 snakehead fish. The fish in KA, KB, KC and KD groups were injected with aqueous extract of johar leaves with concentrations of 10%, 20%, 40%, and 60% respectively, while in KE group the fish was injected with distilled water (control). The parameters of this study were the survival rate and blood profile of snakehead fish. Blood hematology examination included calculation of erythrocyte count, hemoglobin level, and hematocrit percentage, leukocyte count, and thrombocyte count. The research data obtained were analyzed using one-way analysis of variance and followed by Duncan's Multiple Distance test. Injection of aqueous extract of johar leaf did not affect the survival rate of snakehead fish. However, hemoglobin and platelet levels significant decrease in the group given 60% concentration of aqueous extract of johar leaf. It can be concluded that aqueous extract of johar leaf at a concentration of 10%, 20%, 40% is safe to use in snakehead fish.Keywords:Johar leaves extractSnakehead fishBlood profileSurvival rate
Generation of higher-order Laguerre–Gaussian modes from a diode-pumped Pr3+:LiYF4 laser with an intra-cavity spherical aberration
Authors: Rao A. Srinivasa , Morohashi Takuya , Kerridge-Johns William R. , Omatsu Takashige 
Published: January 2023

We demonstrate, for the first time to the best of our knowledge, the direct generation of higher-order Laguerre–Gaussian (LG) petal modes, formed of the coherent superposition of positive and negative LG modes with the topological charge of

±

ℓ

as an eigen mode, from a compact diode end-pumped

P
r

3
+

:

L
i
Y
F

4

(YLF) laser with an intra-cavity lens configuration. The on-axis displacement of the intra-cavity lens with spherical aberration allows the selective operation of the desired higher-order LG modes with

|

ℓ

|

≤

31

from the laser cavity at 640 nm and 607 nm. Such visible higher-order LG modes will offer new applications in optical manipulation, quantum/optical underwater telecommunication, and microfabrication.