bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Lambda-evo (λevo) phage platform for Zika virus EDIII protein display
Authors: Negrete-Méndez Honorio , Valencia-Toxqui Guadalupe , Martínez-Peñafiel Eva , Medina-Contreras Oscar , Fernández-Ramírez Fernando , Morales-Ríos Edgar , Navarro-González Luis Janiel , Torres-Flores Jesús M. , Kameyama Luis 
Published: January 2025

Abstract
One of the most significant bacteriophage technologies is phage display, in which heterologous peptides are exhibited on the virion surface. This work describes the display of λ decorative protein Dλ linked to the E protein domain III of Zika virus (Dλ-ZEDIII), to the GFP protein (Dλ-GFP), or to different domain III epitopes of the EZIKV protein (Dλ-TD), exhibited on the surface of an in vitro evolved lambda phage (λevo). This phage harbors a gene D deletion and was subjected to directed evolution using Escherichia coli W3110/pDλ-ZEDIII as background. After 20 days (20 cycles of dilution), the λevo phage developed a ~ 22% genome deletion affecting the non-essential λ b region, rendering a more stable phage that exhibited fusion proteins Dλ-ZEDIII or Dλ-GFP but not Dλ-TD. Despite the λevo system was able to decorate itself with the Dλ-ZEDIII protein, the production of viral particles was ~ 1000-fold lower than the λ wild-type, due to the unexpected Dλ-ZEDIII protein aggregation into bacterial inclusion bodies. Decorated phages (106 PFU (plaque forming units)/100 µl) were inoculated into BALB/c mice, and subsequent dot blot and Western blot immunoassays proved the production of murine antibodies against ZIKV (Zika virus). This multipurpose λevo phage display platform may be used interchangeably with other more soluble peptides, providing better yields.

Key points
• λ

evo

platform for displaying recombinant peptides.

• Directed evolution to generate λ

evo

with more efficient decoration.

• Antigenic reaction in BALB/c mice by inoculating λ

evo

with recombinant peptides.
Current advancements in cellular immunotherapy for autoimmune disease
Authors: Berry Corbett T. , Frazee Caitlin S. , Herman Patrick J. , Chen Sisi , Chen Anna , Kuo Yvonne , Ellebrecht Christoph T. 
Published: January 2025

Abstract
The management of autoimmune diseases is currently limited by therapies that largely suppress the immune system, often resulting in partial and temporary remissions. Cellular immunotherapies offer a targeted approach by redirecting immune cells to correct the underlying autoimmunity. This review explores the latest advances in cellular immunotherapies for autoimmune diseases, focusing on various strategies, such as the use of chimeric antigen receptor (CAR) T cells, chimeric auto-antibody receptor (CAAR) T cells, regulatory T cells (Tregs), and tolerogenic dendritic cells (TolDCs). We review recent preclinical studies and results from clinical trials that demonstrate the potential for these therapies to either deplete autoreactive cells or promote immune tolerance through broad or selective targeting of immune cell populations. Key challenges such as ensuring specificity, preventing off-target effects, and improving the longevity of therapeutic effects are discussed. The evolving landscape of cellular immunotherapies holds promise for more durable treatment responses and increased specificity for autoimmune disease treatment.
Transcriptional activation and coactivator binding by yeast Ino2 and human proto-oncoprotein c-Myc
Authors: Wendegatz Eva-Carina , Lettow Julia , Wierzbicka Wiktoria , Schüller Hans-Joachim 
Published: January 2025

Abstract
Basic helix-loop-helix domains in yeast regulatory proteins Ino2 and Ino4 mediate formation of a heterodimer which binds to and activates expression of phospholipid biosynthetic genes. The human proto-oncoprotein c-Myc (Myc) and its binding partner Max activate genes important for cellular proliferation and contain functional domains structure and position of which strongly resembles Ino2 and Ino4. Since Ino2—Myc and Ino4—Max may be considered as orthologs we performed functional comparisons in yeast. We demonstrate that Myc and Max could be stably synthesized in S. cerevisiae and together significantly activated a target gene of Ino2/Ino4 but nevertheless were unable to functionally complement an ino2 ino4 double mutant. We also map two efficient transcriptional activation domains in the N-terminus of Myc (TAD1: aa 1–41 and TAD2: aa 91–140), corresponding to TAD positions in Ino2. We finally show that coactivators such as TFIID subunits Taf1, Taf4, Taf6, Taf10 and Taf12 as well as ATPase subunits of chromatin remodelling complexes Swi2, Sth1 and Ino80 previously shown to interact with TADs of Ino2 were also able to bind TADs of Myc, supporting the view that heterodimers Ino2/Ino4 and Myc/Max are evolutionary related but have undergone transcriptional rewiring of target genes.
Evaluation of an e-self-management intervention (Happy Hands app) for hand osteoarthritis: Study protocol for a multicentre randomised controlled trial
Authors: Tveter Anne Therese , Fjeldstad Kristine Aasness , Varsi Cecilie , Maarnes Marit Kristin , Pedersen Stein Jarle , Christensen Barbara S. , Blanck Thalita , Nyheim Sissel B. , Prior Yeliz , Björk Mathilda , Pelle Tim , Kjeken Ingvild 
Published: January 2025

Abstract

Objective
This protocol paper describes the rationale and design of a randomised controlled trial (RCT) that aims to evaluate the (cost-)effectiveness of a 12 week e-self-management intervention (Happy Hands app) in people with hand osteoarthritis (HOA).

Methods
In this multicentre RCT, 376 people with HOA will be recruited from all four health regions in Norway. Consenting participants will be randomly allocated to either a control group receiving usual care or an intervention group receiving the Happy Hands app in addition to usual care. Primary outcome will be measured at 3-months follow-up as the proportion of participants classified as OMERACT-OARSI responders (a composite score comprising change in pain, function, and disease activity), analysed using logistic regression. Secondary outcomes, including pain, hand function, stiffness, quality-of-care, health-related quality-of-life, grip strength, adherence and healthcare costs will be measured at 3- and 6-months follow-up.

Results
Recruitment was initiated in November 2022 with a total of 386 participants recruited by August 2023, 194 in the intervention group and 192 in the control group. Data collection was completed in February 2024.

Discussion
To our knowledge, this is one of the first large-scale, multicentre RCTs assessing the (cost-)effectiveness of a self-management program delivered through a smartphone app for people with HOA. The results from this trial can enhance our understanding of the role technology can play in managing HOA.

Trial registration
NCT05568875 (https://clinicaltrials.gov/study/NCT05568875, pre-registered October 3, 2022).
Fine resolution satellite sea surface temperatures capture the conditions experienced by corals at monthly but not daily timescales
Authors: Bos Jaelyn T. , Pinsky Malin L. 
Published: January 2025

Abstract
Water temperature is a strong driver of growth, survival, and local adaptation in corals, but our knowledge of the temperatures experienced by corals on reefs worldwide remains limited. While in situ temperature loggers can provide high quality data, they are relatively expensive to place and retrieve. Alternatively, remotely sensed sea surface temperature data are globally available but may be a biased representation of the temperatures experienced by corals. Here, we compared data from 314 temperature loggers on coral reefs to the ~ 1 km2 resolution remotely sensed Multi-scale Ultra-high Resolution Sea Surface Temperature (MUR) product from NASA. We found good agreement (Pearson’s r = 0.95) between maximum monthly mean temperatures calculated from remote and in situ data, with 84% of temperatures within 0.5 °C of each other. However, remotely sensed temperature did not effectively capture sub-diel temperature fluctuations and the highest peak temperatures that may be most dangerous for corals. Predictions of in situ temperatures were significantly but weakly improved by a consideration of reef geomorphology. Ultimately, we found that remotely sensed temperatures can accurately represent the monthly conditions experienced by most corals but should be used with caution at finer temporal scales.
Impact of microplastics on female reproductive health: insights from animal and human experimental studies: a systematic review
Authors: Inam Özen 
Published: January 2025

Abstract

Objective
This systematic review aims to evaluate the impact of microplastics on female reproductive health by analyzing experimental studies.

Method
A comprehensive search was conducted in PubMed, Web of Science, and Scopus databases to identify experimental studies published between 2021 and 2023. Studies investigating the effects of microplastics on reproductive organs, hormone levels, fertility rates, and offspring development in female subjects were included. The quality of the studies was assessed using the Cochrane risk of bias tool.

Results
A total of 15 studies met the inclusion criteria. The results indicate that exposure to microplastics significantly affects ovarian function, decreases fertility rates, and disrupts hormone levels in female subjects. Several studies also reported negative effects on embryo development and offsprings health. The quality of the studies varied, with some showing a high risk of bias.

Conclusion
The evidence from experimental studies suggests that microplastics have a detrimental effect on female reproductive health. However, the variation in study quality highlights the need for more rigorous research to confirm these results and better understand the underlying mechanisms.
Improving detection of monogenic diabetes through reanalysis of GCK variants of uncertain significance
Authors: De Sousa Sunita M. C. , Phan Jennifer M. N. , Wells Amanda , Wu Kathy H. C. , Scott Hamish S. 
Published: January 2025

Abstract

Aims
To assess the utility of reanalysing GCK variants of uncertain significance (VUS) as an intervention to improve the detection of monogenic diabetes.

Methods
We examined GCK VUS in a local cohort of individuals with suspected monogenic diabetes and re-curated each variant against the recent ClinGen GCK-specific variant classification guidelines.

Results
Variant reanalysis achieved a new ‘likely pathogenic’ classification (i.e., positive results) in 4/8 identified VUS. The single most common newly applied criterion indicating variant pathogenicity was a confirmed phenotype of GCK-hyperglycaemia. RNA sequencing and segregation studies were performed in two cases but not additive to reclassification.

Conclusions
This is the first VUS reclassification study in monogenic diabetes using gene-specific guidelines. Within the limits of this small study, we observed a high rate (50%) of VUS upgrades to a positive result, thereby confirming the utility of VUS reanalysis– particularly with biochemical phenotyping– in increasing the detection of monogenic diabetes. We recommend HbA1c, fasting blood glucose and either pancreatic autoantibody negativity or a small oral glucose tolerance test increment as a feasible minimum dataset to inform variant classification at the individual patient level, noting the ongoing work of the ClinGen Monogenic Diabetes Expert Panel in systematically reviewing GCK variants at the international level.
In vivo 9.4 Tesla MRI of a patient with drug-resistant epilepsy: Technical report
Authors: van Lanen Rick H. G. J. , Uher Daniel , Tse Desmond H. Y. , Steijvers Esther , Colon Albert J. , Jansen Jacobus F. A. , Drenthen Gerhard S. , Ivanov Dimo , Hoogland Govert , Rijkers Kim , Hoeberigs Christianne M. , Hofman Paul A. M. , Backes Walter H. , Schijns Olaf E. M. G. 
Published: January 2025

Abstract

Purpose
In resective epilepsy surgery for drug-resistant focal epilepsy (DRE), good seizure outcome is strongly associated with visualization of an epileptogenic lesion on MRI. Standard clinical MRI (≤ 3 Tesla (T)) may fail to detect subtle lesions. 7T MRI enhances detection and delineation, the potential benefits of increasing field strength to 9.4T are explored.

Methods
A 36 years old male patient with DRE evaluated for resective surgery, in which 3T and 7T MRI failed to detect any epileptogenic lesions, was submitted to a dedicated epilepsy scan protocol using T1 and T2* weighted imaging at 9.4T. Images were evaluated independently by two neuroradiologists and one neurosurgeon.

Results
9.4T MRI offered increased spatial resolution and enhanced depiction of anatomical structures vital for epilepsy imaging, exemplified by regions mesio-temporal (hippocampus, amygdala), latero-temporal, insula, frontal and temporal operculum, and gray-white matter junction (precentral gyrus/frontal lobe) compared to 3T and 7T, albeit with challenges in mesial-temporal and antero-inferior temporal lobe imaging. No epileptogenic lesion was identified.

Conclusion
9.4T demonstrates promise in the identification and delineation of anatomical structures and small epileptogenic lesions in patients with DRE eligible for resective surgery. Whether clinical 9.4T MRI in DRE has clinical advantages over 7T or leads to a more complete resection of the epileptogenic zone and improved seizure outcome after epilepsy surgery needs to be established.
Fundraising, Governance and Environmental Ethics: Evidence from Equity Crowdfunding
Authors: Vismara Silvio , Wirtz Peter 
Published: January 2025

Abstract
There is an important research tradition concerning the financial implications of social and environmental ethics. This study investigates the short- and long-term financial performance of ventures with explicit environmental commitments seeking to raise funds in equity crowdfunding (ECF) markets. Our results indicate that environmental orientation positively influences short-term funding performance, though only when accompanied by the costly signal of robust corporate governance mechanisms. In the long run, environmental orientation also positively impacts performance, albeit with only weak statistical significance when observed alone. Governance again serves as a positive moderator, but its effect is less significant relative to the short term. However, environmental orientation does feature a strongly significant positive impact on long-run performance when combined with a successfully conducted initial ECF campaign.
A solar-powered, internet of things (IoT)-controlled water irrigation system supported by rainfall forecasts utilizing aerosols: a review
Authors: Balamurali D. , Chakankar Samarth , Sharma Giridhar , Pagey Ananjan Praveen , Natarajan M. , Shaik Saboor , Gnanavendan Sudarshan , Arıcı Müslüm 
Published: January 2025

Abstract
Efficient water management is crucial in modern agriculture, especially in regions facing water scarcity. Traditional irrigation systems often result in water wastage, which challenges sustainability goals. This paper presents a comprehensive review of a novel Internet of Things (IoT)-based smart irrigation system with rainfall prediction based on pollutant concentration designed to optimize water usage through real-time environmental monitoring and promote sustainable agriculture through the integration of photovoltaic (PV) power. The system's components, including 160 Wp semi-crystalline PV panels, ESP8266 Wi-Fi module, Arduino Uno, Raspberry Pi, Partial Maximum Power Point Tracking (MPPT) control methods, and a reversible pump, were selected based on a comprehensive literature review, establishing the foundation for the proposed system’s design. The review extensively covers previous PV-irrigation integration systems, their performance in varied environments, and the cost–benefit analysis with special reference to Indian government subsidies for solar adoption in agriculture. In addition, various IoT-based irrigation systems and communication technologies such as Wi-Fi, LoRa, and Zigbee were reviewed, ensuring that the selected components represent the most efficient and secure combination for field deployment. The key novelty of this system is the rainfall prediction methodology, which focuses on pollutant concentration (PM10, PM2.5, SO₂, NO₂, CO, NH3, Cr and Pb) rather than the traditional aerosol size used in previous studies. This approach leverages the unique environmental characteristics of the chosen location, providing more accurate predictions of rainfall, which can be used to dynamically adjust irrigation schedules, reducing water waste. Sample data of pollutant levels and weather conditions from the area are provided as a demonstration. The paper also reviews security concerns in IoT systems, highlighting potential vulnerabilities and mitigation strategies to ensure robust system operation. By combining IoT, PV, and predictive weather analytics, the proposed system offers significant advantages in water and energy management, with the MPPT controller maximizing solar energy efficiency. The review presents a detailed flow diagram of the system, showcasing components chosen through a thorough literature survey. It also examines portable PV-pumping systems, recommending the proposed design for effective use in both small- and medium-scale farms. This paper provides a comprehensive analysis of sustainable irrigation technologies, highlighting their potential to significantly improve agricultural water management practices.
Interpretation of the Klein-Gordon Probability Density
Authors: Sutherland Roderick I. 
Published: January 2025

Abstract
The fact that the “probability density” expression provided by the Klein–Gordon equation can take on negative values is usually seen as an obstacle to formulating a particle interpretation of quantum mechanics. Nevertheless, reconciling this expression with a particle ontology is quite possible once a careful distinction is drawn between the outcomes of measurements and the positions of particles between measurements. Following this path, however, points to the involvement of retrocausality, as proposed by various authors in other contexts.
From waste to wealth: coupling different nutritional modes of Scenedesmus obliquus for waste remediation and algal product development
Authors: Silkina Alla , Gayo-Peláez José Ignacio , Fernandes Fleuriane , Fuentes-Grünewald Claudio , Kapoore Rahul Vijay , Tang Kam W. 
Published: January 2025

Abstract
This study exploited the different combinations of nutritional modes (autotrophic, mixotrophic and heterotrophic) of the green alga Scenedesmus obliquus in a two-stage cultivation process to remediate wastes as well as maximise the production of high-protein algal biomass. The alga was first cultivated autotrophically in Stage-one, using 0.5% of digestate as a nutrient source and natural light to support the growth; afterward, the culture was concentrated and used in Stage-two cultivation under the different nutritional modes. 2.5% of anaerobic digestate from food waste was used to provide inorganic nutrients; waste sugar recovered from a confectionery manufacturer was used as the organic carbon source to support mixo- and hetero-trophic growth. In Stage-one, the algae removed ammonium and phosphate at 9.1 mg L−1 day−1 and 3.8 mg L−1 day−1, respectively, and the algal yield approached a carrying capacity of 1 g dry weight per liter. In Stage-two, the algal population increased faster in mixotrophic and heterotrophic modes, reaching a final dry weight concentration of 7.2–10.5 g L−1, than in autotrophic mode. Ammonium and phosphate were consumed at about twice the rate as in autotrophic mode, and 90–100% of the waste sugar was removed within 48–72 h. The final algal biomass had a protein content of 44% in mixotrophic mode, compared to only 32% in autotrophic mode. This study demonstrated that a two-stage cultivation process coupling autotrophic and mixotrophic growth of S. obliquus can be used to remediate both inorganic and organic wastes and boost the production of protein-rich algal biomass, which can be beneficial to a sustainable circular bioeconomy.
Earthquakes and seismic hazard for Norway and Svalbard
Authors: Lindholm Conrad , Bungum Hilmar , Ghione Federica , Meslem Abdelghani , Huang Chen , Oye Volker 
Published: January 2025

Abstract
We document a complete seismic hazard study for mainland Norway and the Svalbard archipelago. The study is based on a Probabilistic Seismic Hazard Analysis (PSHA) method, and for the first time a new earthquake catalogue is presented publicly that covers Norway, Svalbard and the adjacent offshore regions. The catalogue is developed from an extensive analysis of historical earthquakes combined with more recent instrumental data with 33,864 reports between 1497 through 2018, and with magnitudes up to Mw 6.7. With this catalogue seismic hazard is computed for 10% exceedance in 475 years through a logic tree computation with 12 branches: two area-zonations, one zonation free branch and four GMPEs. These 12 branches were defined with the aim to reduce the model bias, i.e., to centre the model, and to capture the epistemic uncertainty of the results. While the conventional Vs30 reference velocity is usually around 800 m/s we have targeted a reference velocity of 1200 m/s, based on extensive documentation of Norwegian rock velocities. This has significant bearing on the calculated hazard and provides for results that better reflect the bedrock conditions in Norway. As a result of this, the predicted shaking intensities are lower than the values previously reported in the (1998) national building code. In the Supplementary Information we have provided a brief overview of the seismotectonic setting, some tests that further demonstrate the uncertainty in our hazard estimates, a model for H/V ground-motion response spectra, examples of the sensitivity to the bedrock reference velocity and a comparison between the present study and the ESHM20 results.
Genomic regions associated with spot blotch resistance in elite barley breeding populations
Authors: Roy Dipika , Dinglasan Eric , Fowler Ryan , Platz Greg , Lance Reg , Synman Lisle , Franckowiak Jerome , Hickey Lee Thomas , Voss-Fels Kai , Robinson Hannah 
Published: January 2025

Abstract
Spot blotch (SB), a prevalent foliar disease of barley, is caused by the hemibiotrophic fungal pathogen Bipolaris sorokiniana. Predominately occurring in humid growing regions worldwide, SB can result in yield losses of up to 30%. Genetic resistance remains the most effective strategy for disease management; however, most Australian barley cultivars exhibit susceptibility despite the previous identification of major resistance loci. This study investigates the genetic architecture underlying spot blotch resistance within an Australian barley breeding program. Resistance was assessed at both the seedling and adult growth stages using a single conidial isolate (SB61) across two consecutive years. A total of 337 barley lines were genotyped with 16,824 polymorphic DArT-seq™ markers. Two mapping approaches were employed: a single-marker genome-wide association study (GWAS) and a haplotype-based local genomic estimated breeding values (Local GEBV) approach. Both methodologies identified two major resistance-associated regions on chromosomes 3H and 7H, effective across growth stages. Additionally, the haplotype-based Local GEBV approach revealed resistance-associated regions on 1H, 3H, and 6H that were not detected by GWAS. Haplotype stacking analysis underscored the critical role of the 7H region for adult-plant resistance when combined with other resistance haplotypes, suggesting significant gene-by-gene interactions and highlighting the complex, quantitative nature of spot blotch resistance. This research confirms the presence of key resistance loci within Australian barley breeding populations, provides novel insight into the genetic architecture of spot blotch resistance, and emphasises the potential to enhance resistance through haplotype stacking and whole-genome prediction approaches.