bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of a method for the dry preservation and rehydration of Anopheles gambiae sensu lato for parity analysis to assess the impact of vector control measures in the field
Authors: Pretorius Elizabeth , Kristan Mojca , Bradley John , da Silva Eunice Teixeira , Hutchins Harry , Barri Fatucha , Cassama Ansumane , Ceesay Sainey , Ndiath Mamadou Ousmane , Rodrigues Amabelia , Logan James G. , Last Anna , Jones Robert T. 
Published: July 2023

Abstract
Background
As the control of malaria remains heavily dependent on vector management interventions, it is important to understand the impact of these on mosquito populations. Age-grading is a valuable tool for this; however, logistical challenges in remote, resource-poor areas make current methodologies difficult to incorporate into clinical trials and routine surveillance. Our aim was to validate a methodology that could be easily implemented in such settings. Using dried mosquito specimens instead of freshly killed ones, we validated the commonly used ovarian tracheation technique for assessing population age structure.

Methods
Laboratory-reared Anopheles coluzzii mosquitoes with known parity status were dry preserved in silica gel for up to 12 weeks and rehydrated prior to parity assessment. The results were compared to parity results for freshly killed mosquitoes from the same colony. Preserved, field-caught Anopheles gambiae sensu lato (s.l.) from Guinea-Bissau were assessed by three different assessors blinded to each other’s scores. An overall index of agreement was calculated using inter-rater reliability of all assessor pairings. The impact of preservation time was investigated using a one-way ANOVA to look for differences in assessor agreement over three time periods.

Results
The parity status was correctly identified for 90% of dry preserved and rehydrated insectary-reared An. coluzzii and for 98% of freshly killed insectary-reared An. coluzzii. The inter-rater reliability was highest (0.94) for freshly killed An. coluzzii. The results for all time points showed excellent strength of agreement between assessors. For field-caught An. gambiae s.l., the overall index of agreement between all three assessors was 0.86 (95% confidence interval 0.78–0.93), indicating almost perfect agreement. There was no significant difference between assessor agreement between time frames.

Conclusions
Dry preserving and rehydrating Anopheles mosquitoes provides an alternative to using freshly killed mosquitoes to assess the efficacy of a control intervention in remote settings where it is logistically difficult to dissect fresh specimens. This method also provides the flexibility required for parity assessment to be done on larger scales over bigger areas.

Graphical Abstract
A longitudinal follow-up of continuous laryngoscopy during exercise test scores in athletes irrespective of laryngeal obstruction, respiratory symptoms, and intervention
Authors: Irewall Tommie , Bäcklund Catharina , Naumburg Estelle , Ryding Marie , Stenfors Nikolai 
Published: July 2023

Abstract
Background
Exercise-induced laryngeal obstruction (EILO) is diagnosed by the continuous laryngoscopy during exercise (CLE) test. Whether or how much CLE test scores vary over time is unknown. This study aimed to compare CLE test scores in athletes over time, irrespective of respiratory symptoms and grade of laryngeal obstruction.

Methods
Ninety-eight athletes previously screened for EILO were invited for a follow-up CLE test irrespective of CLE scores and respiratory symptoms. Twenty-nine athletes aged 16–27 did a follow-up CLE test 3–23 months after the baseline test. Laryngeal obstruction at the glottic and supraglottic levels was graded by the observer during exercise, at baseline and follow-up, using a visual grade score (0–3 points).

Results
At baseline, 11 (38%) of the 29 athletes had moderate laryngeal obstruction and received advice on breathing technique; among them, 8 (73%) reported exercise-induced dyspnea during the last 12 months. At follow-up, 8 (73%) of the athletes receiving advice on breathing technique had an unchanged supraglottic score. Three (17%) of the 18 athletes with no or mild laryngeal obstruction at baseline had moderate supraglottic obstruction at follow-up, and none of the 3 reported exercise-induced dyspnea.

Conclusions
In athletes with repeated testing, CLE scores remain mostly stable over 3–24 months even with advice on breathing technique to those with EILO. However, there is some intraindividual variability in CLE scores over time.

Trial registration
ISRCTN, ISRCTN60543467, 2020/08/23, retrospectively registered, ISRCTN – ISRCTN60543467: Investigating conditions causing breathlessness in athletes.
Benefits of combined use of 68−Ga Dotatoc and 5-ALA fluorescence for recurrent atypical skull-base meningioma after previous microsurgery and Gamma Knife radiosurgery: a case report
Authors: Peciu-Florianu Iulia , Jaillard Alice , Tuleasca Constantin , Reyns Nicolas 
Published: July 2023

Abstract
Background
Studies of novel microsurgical adjuncts, such as 5-aminolevulinic acid (5-ALA) fluorescence have shown various fluorescence patterns within meningiomas, opening new avenues for complete microsurgical resection. Here, we present a recurrent, radiation-induced meningioma, previously operated on two occasions (initial gross total resection and subtotal 12 years later) and also irradiated by Gamma Knife radiosurgery (GKR, 6 years after the first surgery). We thought to assess the usefulness of 68−Ga Dotatoc in surgical target planning and of 5-ALA as an adjunct for maximal microsurgical excision.

Case report
We report on a 43 years-old Caucasian male diagnosed with atypical, radiation induced WHO II meningioma, with left basal temporal bone implantation. Hodgkin lymphoma treated with cranial and mediastinal radiation during infancy marked his personal history. He underwent a first gross total microsurgical resection, followed 6 and 12 years later by Gamma Knife radiosurgery (GKR) and second subtotal microsurgical resection, respectively. Magnetic resonance imaging (MRI) displayed new recurrence 13 years after initial diagnosis. He was clinically asymptomatic but routine Magnetic resonance imaging showed constant progression. There was strong 68−Ga Dotatoc uptake. We used 5-ALA guided microsurgical resection. Intraoperative views confirmed strong fluorescence, in concordance with both preoperative Magnetic resonance imaging enhancement and 68−Ga Dotatoc. The tumor was completely removed, with meningeal and bone resection.

Conclusion
The authors conclude that fluorescence-guided resection using 5-ALA is useful for recurrent atypical, radiation-induced meningioma even despite previous irradiation and multiple recurrences.
Automatic healthy liver segmentation for holmium-166 radioembolization dosimetry
Authors: Stella Martina , van Rooij Rob , Lam Marnix G. E. H. , de Jong Hugo W. A. M. , Braat Arthur J. A. T. 
Published: July 2023

Abstract
Background
For safe and effective holmium-166 (166Ho) liver radioembolization, dosimetry is crucial and requires accurate healthy liver definition. The current clinical standard relies on manual segmentation and registration of a separately acquired contrast enhanced CT (CECT), a prone-to-error and time-consuming task. An alternative is offered by simultaneous imaging of 166Ho and technetium-99m stannous–phytate accumulating in healthy liver cells (166Ho–99mTc dual-isotope protocol). This study compares healthy liver segmentation performed with an automatic method using 99mTc images derived from a 166Ho–99mTc dual-isotope acquisition to the manual segmentation, focusing on healthy liver dosimetry and corresponding hepatotoxicity. Data from the prospective HEPAR PLuS study were used. Automatic healthy liver segmentation was obtained by thresholding the 99mTc image (no registration step required). Manual segmentation was performed on CECT and then manually registered to the SPECT/CT and subsequently to the corresponding 166Ho SPECT to compute absorbed dose in healthy liver.

Results
Thirty-one patients (66 procedures) were assessed. Manual segmentation and registration took a median of 30 min per patient, while automatic segmentation was instantaneous. Mean ± standard deviation of healthy liver absorbed dose was 18 ± 7 Gy and 20 ± 8 Gy for manual and automatic segmentations, respectively. Mean difference ± coefficient of reproducibility between healthy liver absorbed doses using the automatic versus manual segmentation was 2 ± 6 Gy. No correlation was found between mean absorbed dose in the healthy liver and hepatotoxicity.

Conclusions
166Ho–99mTc dual-isotope protocol can automatically segment the healthy liver without hampering the 166Ho dosimetry assessment.
Trial registration: ClinicalTrials.gov, NCT02067988. Registered 20 February 2014. https://clinicaltrials.gov/ct2/show/NCT02067988
Isolation of Zygosaccharomyces siamensis kiy1 as a novel arabitol-producing yeast and its arabitol production
Authors: Iwata Kan , Maeda Mayumi , Kashiwagi Yutaka , Maehashi Kenji , Yoshikawa Jun 
Published: July 2023

AbstractArabitol is gaining attention in the food industry as an alternative sweetener owing to its low-caloric and non-cariogenic characteristics. The yeast strain kiy1 was newly isolated from unpasteurized honey for arabitol production. Based on internal transcribed spacer sequence analysis, the isolated strain was identified as Zygosaccharomyces siamensis. In this study, the effects of different substrates and sugar concentrations on arabitol production were investigated. When three types of carbon sources (glycerol, fructose, and glucose) were used, glucose was the most suitable substrate for arabitol production (68.7 g/L). Maximum arabitol production (101.4 g/L) was observed at a glucose concentration of 30%, and the highest arabitol production yield was 0.34 g/g of initial glucose. In the time-course production of sugar alcohols by strain kiy1, glucose was completely consumed for 8 days. The concentration of arabitol exceeded that of glycerol after 3 days, and the final arabitol concentration reached 83.6 g/L after 10 days. The maximum production rate was 16.7 g/L/day. The yeast produced glycerol as an intracellular sugar alcohol in the early stage of culture and switched its metabolism to arabitol production after the middle stage. Z. siamensis kiy1 possessed an NADP+-dependent arabitol dehydrogenase, which indicated that it probably produces arabitol via ribulose from glucose. These results suggest that the novel yeast strain, Z. siamensis kiy1, is promising for arabitol production. The proposed arabitol production approach can contribute toward its production at the industrial scale.
Graphical Abstract
SEFSD: an effective deployment algorithm for fog computing systems
Authors: Chen Huan , Chang Wei-Yan , Chiu Tai-Lin , Chiang Ming-Chao , Tsai Chun-Wei 
Published: July 2023

AbstractFog computing aims to mitigate data communication delay by deploying fog nodes to provide servers in the proximity of users and offload resource-hungry tasks that would otherwise be sent to distant cloud servers. In this paper, we propose an effective fog device deployment algorithm based on a new metaheuristic algorithm–search economics–to solve the optimization problem for the deployment of fog computing systems. The term “effective” in this paper refers to that the developed algorithm can achieve better performance in terms of metrics such as lower latency and less resource usage. Compared with conventional metaheuristic algorithms, the proposed algorithm is unique in that it first divides the solution space into a set of regions to increase search diversity of the search and then allocates different computational resources to each region according to its potential. To verify the effectiveness of the proposed algorithm, we compare it with several classical fog computing deployment algorithms. The simulation results indicate that the proposed algorithm provides lower network latency and higher quality of service than the other deployment algorithms evaluated in this study.
Sitting vs. supine ultrasound measurements of the vastus medialis: correlations with MRI measurements and age considerations
Authors: Taniguchi Masashi , Fukumoto Yoshihiro , Yagi Masahide , Hirono Tetsuya , Yamagata Momoko , Nakai Ryusuke , Yamada Yosuke , Kimura Misaka , Ichihashi Noriaki 
Published: July 2023

Abstract
Background
Muscle thickness (MT) and echo intensity (EI) measurements are ultrasound alternatives to magnetic resonance imaging (MRI) for evaluating muscle quantity and quality. The vastus medialis (VM) is a clinically important muscle, and assessment methods that most accurately reflect its quantity and quality are required. This study aimed to examine the correlation between MT and EI measured in the supine and sitting postures with corresponding MRI-measured muscle quantity and quality indices.

Methods
In total, 134 adults (91 older and 43 young) participated in this study. Ultrasound images of the VM were acquired in the supine and sitting postures, and MT and EI were measured. The cross-sectional area (CSA), muscle volume (MV), and intramuscular adipose tissue (intraMAT) of the VM were evaluated from MRI images using T1-weighted and Dixon methods. Pearson’s coefficients were used to quantify the correlation strength amongst pairs of dependent variables. Meng’s test was used to test for correlation coefficient differences between the two measurement postures (supine and sitting).

Results
The correlation coefficients amongst MT, CSA, and MV were significantly higher in the sitting posture than in the supine posture. EI measured in the supine and sitting postures correlated significantly with intraMAT, and in young individuals, these correlation coefficients were significantly higher in the sitting posture than in the supine posture.

Conclusions
These findings suggest that assessment of VM muscle quantity in the sitting posture is superior for young and older individuals, and assessment of VM muscle quality in the sitting posture is most effective in younger individuals.
Knowledge, attitudes, and perceptions of the multi-ethnic population of the United Arab Emirates on genomic medicine and genetic testing
Authors: Rahma Azhar T. , Ali Bassam R. , Patrinos George P. , Ahmed Luai A. , Elbarazi Iffat , Abdullahi Aminu S. , Elsheik Mahanna , Abbas Maram , Afandi Farah , Alnaqbi Aisha , Al Maskari Fatma 
Published: July 2023

Abstract
Introduction
The adoption and implementation of genomic medicine and pharmacogenomics (PGx) in healthcare systems have been very slow and limited worldwide. Major barriers to knowledge translation into clinical practice lie in the level of literacy of the public of genetics and genomics. The aim of this study was to assess the knowledge, attitudes, and perceptions of the United Arab Emirates (UAE) multi-ethnic communities toward genomic medicine and genetic testing.

Method
A cross-sectional study using validated questionnaires was distributed to the participants. Descriptive statistics were performed, and multivariable logistic regression models were used to identify factors associated with knowledge of genomics.

Results
757 individuals completed the survey. Only 7% of the participants had a good knowledge level in genetics and genomics (95% CI 5.3–9.0%). However, 76.9% of the participants were willing to take a genetic test if their relatives had a genetic disease. In addition, the majority indicated that they would disclose their genetic test results to their spouses (61.5%) and siblings (53.4%).

Conclusions
This study sets the stage for the stakeholders to plan health promotion and educational campaigns to improve the genomic literacy of the community of the UAE as part of their efforts for implementing precision and personalized medicine in the country.
Accelerometer sampling requirements for animal behaviour classification and estimation of energy expenditure
Authors: Yu Hui , Muijres Florian T. , te Lindert Jan Severin , Hedenström Anders , Henningsson Per 
Published: July 2023

Abstract
Background
Biologgers have contributed greatly to studies of animal movement, behaviours and physiology. Accelerometers, among the various on-board sensors of biologgers, have mainly been used for animal behaviour classification and energy expenditure estimation. However, a general principle for the combined sampling duration and frequency for different taxa is lacking. In this study, we evaluated whether Nyquist–Shannon sampling theorem applies to accelerometer-based classification of animal behaviour and energy expenditure approximation. To evaluate the influence of accelerometer sampling frequency on behaviour classification, we annotated accelerometer data from seven European pied flycatchers (Ficedula hypoleuca) freely moving in aviaries. We also used simulated data to systematically evaluate the combined effect of sampling duration and sampling frequency on the performance of estimating signal frequency and amplitude.

Results
We found that a sampling frequency higher than Nyquist frequency at 100 Hz was needed to classify fast, short-burst behavioural movements of pied flycatcher, such as swallowing food with a mean frequency of 28 Hz. In contrast, high frequency movements with longer durations such as flight could be characterized adequately using much lower sampling frequency of 12.5 Hz. To identify rapid transient prey catching manoeuvres within these flight bouts, again a high frequency sampling at 100 Hz was needed. For both the experimental data of the flycatchers and the simulated data, the combination of sampling frequency and sampling duration affected the accuracy of signal frequency and amplitude estimation. For long sampling durations, the sampling frequency equal to the Nyquist frequency was adequate for accurate signal frequency and amplitude estimation. Accuracy declined with decreasing sampling duration, especially for signal amplitude estimation with up to 40% standard deviation of normalized amplitude difference. To accurately estimate signal amplitude at low sampling duration, a sampling frequency of four times the signal frequency was necessary (two times the Nyquist frequency).

Conclusions
The appropriate sampling frequency of accelerometers depends on the objective of the specific study and the characteristics of the behaviour. For studies with no constraints on device battery and storage, a sampling frequency of at least two times the Nyquist frequency will achieve relative optimal representative of signal information (i.e., frequency and amplitude). For classification and energy expenditure estimation of short-burst behaviours, 1.4 times the Nyquist frequency of behaviour is required.
Recruitment strategies to increase racial and ethnic diversity in anorexia nervosa clinical research
Authors: Strauch Julianne , Agnew Alexandra , Meenaghan Erinne , Miller Karen K. , Haines Melanie S. 
Published: July 2023

Abstract
Objective
Inclusion of underrepresented racial and ethnic groups in eating disorder (ED) research is a critical unmet need, but evidence-based recruitment strategies are lacking. We sought to determine whether methods we had implemented to increase recruitment of underrepresented racial and ethnic groups were successful in improving racial and ethnic diversity in a clinical trial in women with anorexia nervosa.

Method
We implemented new strategies for recruitment of underrepresented racial and ethnic groups in a clinical trial on bone health in women with anorexia nervosa, including leveraging social media, liberalizing language on advertisements to be more inclusive of women who are as yet undiagnosed with the disorder or feel stigmatized by its name, translating advertisements to Spanish, and engaging community health centers. We compared participants’ race and ethnicity in this clinical trial versus two similar prior clinical trials.

Results
The percent of non-White and Hispanic participants who have signed a consent form in our ongoing clinical trial (2021–2023) is higher versus two previous clinical trials on bone health in women with anorexia nervosa (2011–2019) with similar inclusion/exclusion criteria and endpoints [non-White: 11/38 (28.9%) vs. 11/188 (5.9%), Hispanic: 6/38 (15.8%) vs. 5/188 (2.7%), p ≤ 0.006]. There was no change in the percent of Black participants [0/38 (0%) vs. 1/188 (0.5%), p = 1.0].

Discussion
Viewing clinical research recruitment through a diversity, equity, and inclusion lens can improve racial and ethnic diversity in ED research. Further research recruitment strategies are needed to be more inclusive of Black populations.
Attachment and parental bond: impact on psychopathology, mental health and quality of life of hemodialysis patients: a cross-sectional study
Authors: De Pasquale Concetta , Pistorio Maria Luisa , Veroux Massimiliano , Sapienza Gabriella , Florio Alberto , Hichy Zira , Ekser Burcin , Giaquinta Alessia , Veroux Pierfrancesco 
Published: July 2023

Abstract
Background
Attachment theory represents a reference model for understanding better how pre-existing personality factors can influence the coping with some chronic conditions. The onset of a chronic disease can represent a "threat" to the relationships between the subject and parental figures according to the type of bond that already exists. The aim of our study was to explore attachment styles in a sample of hemodialysis patients, hypothesizing that a secure attachment bond can constitute a protective factor for the quality of life and mental health in this type of patients.

Design
We used a cross-sectional design.

Methods
Fifty hemodialysis patients were given the following tests: Attachment Style Questionnaire (ASQ) to assess attachment styles, Parental Bonding Instrument (PBI) to assess parental bonding, Short Form Health Survey-36 (SF-36) for perceived quality of life and Middlesex Hospital Questionnaire (MHQ) to detect key psychological symptoms and relevant traits.

Results
The results showed that secure attachment style correlated with good general health (r = 0.339; p < 0.05), good mental health (r = 0.547; p < 0.001) and mental component scale (r = 0.373; p < 0.05) of SF-36. Secure attachment was also significantly associated with mental health (B = 1.104; p = .002) of the SF-36.

Conclusions
The results confirmed the positive role of a secure attachment style for adequate psychological health. Early identification of patients with dysfunctional attachment styles will make it possible to offer them targeted interventions to improve their ability to accept, adapt and manage the disease and to maintain adequate psychological well-being.