bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers
Authors: Zhang Youxue , Zhen Fang , Sun Yue , Han Bing , Wang Hongyi , Zhang Yuhang , Zhang Huaixi , Hu Jing 
Published: August 2023

Abstract
Background
Breast cancer is the most common cancer affecting women across the world. Tumor endothelial cells (TECs) and malignant cells are the major constituents of the tumor microenvironment (TME), but their origin and role in shaping disease initiation, progression, and treatment responses remain unclear due to significant heterogeneity.

Methods
Tissue samples were collected from eight patients presenting with breast cancer. Single-cell RNA sequencing (scRNA-seq) analysis was employed to investigate the presence of distinct cell subsets in the tumor microenvironment. InferCNV was used to identify cancer cells. Pseudotime trajectory analysis revealed the dynamic process of breast cancer angiogenesis. We validated the function of small extracellular vesicles (sEVs)-derived protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B) in vitro experiments.

Results
We performed single-cell transcriptomics analysis of the factors associated with breast cancer angiogenesis and identified twelve subclusters of endothelial cells involved in the tumor microenvironment. We also identified the role of TECs in tumor angiogenesis and confirmed their participation in different stages of angiogenesis, including communication with other cell types via sEVs. Overall, the research uncovered the TECs heterogeneity and the expression levels of genes at different stages of tumor angiogenesis.

Conclusions
This study showed sEVs derived from breast cancer malignant cells promote blood vessel formation by activating endothelial cells through the transfer of PPP1R1B. This provides a new direction for the development of anti-angiogenic therapies for human breast cancer.
CX3CL1 promotes M1 macrophage polarization and osteoclast differentiation through NF-κB signaling pathway in ankylosing spondylitis in vitro
Authors: Feng Xinzhe , Zhu Shanbang , Qiao Junjie , Ji Zhou , Zhou Bole , Xu Weidong 
Published: August 2023

Abstract
Background
Ankylosing spondylitis (AS) is an autoimmune disease with a genetic correlation and is characterized by inflammation in the axial skeleton and sacroiliac joints. Many AS patients also have inflammatory bowel diseases (IBD), but the underlying causes of intestinal inflammation and osteoporosis in AS are not well understood. CX3CL1, a protein involved in inflammation, has been found to be up-regulated in AS patients and AS-model mice.

Methods
The authors investigated the effects of CX3CL1 on AS by studying its impact on macrophage polarization, inflammation factors, and osteoclast differentiation. Furthermore, the effects of inhibiting the NF-κB pathway and blocking CX3CL1 were assessed using BAY-117082 and anti-CX3CL1 mAb, respectively. AS model mice were used to evaluate the effects of anti-CX3CL1 mAb on limb thickness, spine rupture, and intestinal tissue damage.

Results
The authors found that CX3CL1 increased the expression of M1-type macrophage markers and inflammation factors, and promoted osteoclast differentiation. This effect was mediated through the NF-κB signaling pathway. Inhibition of the NF-κB pathway prevented M1-type macrophage polarization, reduced inflammation levels, and inhibited osteoclast differentiation. Injection of anti-CX3CL1 mAb alleviated limb thickness, spine rupture, and intestinal tissue damage in AS model mice by inhibiting M1-type macrophage polarization and reducing intestinal tissue inflammation.

Conclusions
The study demonstrated that up-regulated CX3CL1 promotes M1-type macrophage polarization and osteoclast differentiation through the NF-κB signaling pathway. Inhibition of this pathway and blocking CX3CL1 can alleviate inflammation and bone destruction in AS. These findings contribute to a better understanding of the pathogenesis of AS and provide a basis for clinical diagnosis and treatment.
SCA: recovering single-cell heterogeneity through information-based dimensionality reduction
Authors: DeMeo Benjamin , Berger Bonnie 
Published: August 2023

AbstractDimensionality reduction summarizes the complex transcriptomic landscape of single-cell datasets for downstream analyses. Current approaches favor large cellular populations defined by many genes, at the expense of smaller and more subtly defined populations. Here, we present surprisal component analysis (SCA), a technique that newly leverages the information-theoretic notion of surprisal for dimensionality reduction to promote more meaningful signal extraction. For example, SCA uncovers clinically important cytotoxic T-cell subpopulations that are indistinguishable using existing pipelines. We also demonstrate that SCA substantially improves downstream imputation. SCA’s efficient information-theoretic paradigm has broad applications to the study of complex biological tissues in health and disease.
p53 amyloid pathology is correlated with higher cancer grade irrespective of the mutant or wild-type form
Authors: Sengupta Shinjinee , Singh Namrata , Paul Ajoy , Datta Debalina , Chatterjee Debdeep , Mukherjee Semanti , Gadhe Laxmikant , Devi Jyoti , Mahesh Yeshwanth , Jolly Mohit Kumar , Maji Samir K. 
Published: August 2023

ABSTRACT
p53 (also known as TP53) mutation and amyloid formation are long associated with cancer pathogenesis; however, the direct demonstration of the link between p53 amyloid load and cancer progression is lacking. Using multi-disciplinary techniques and 59 tissues (53 oral and stomach cancer tumor tissue samples from Indian individuals with cancer and six non-cancer oral and stomach tissue samples), we showed that p53 amyloid load and cancer grades are highly correlated. Furthermore, next-generation sequencing (NGS) data suggest that not only mutant p53 (e.g. single-nucleotide variants, deletions, and insertions) but wild-type p53 also formed amyloids either in the nucleus (50%) and/or in the cytoplasm in most cancer tissues. Interestingly, in all these cancer tissues, p53 displays a loss of DNA-binding and transcriptional activities, suggesting that the level of amyloid load correlates with the degree of loss and an increase in cancer grades. The p53 amyloids also sequester higher amounts of the related p63 and p73 (also known as TP63 and TP73, respectively) protein in higher-grade tumor tissues. The data suggest p53 misfolding and/or aggregation, and subsequent amyloid formation, lead to loss of the tumor-suppressive function and the gain of oncogenic function, aggravation of which might determine the cancer grade.
Actomyosin-dependent cell contractility orchestrates Zika virus infection
Authors: Huang Xinyi , Xing Yifan , Cui Yanqin , Ji Baohua , Ding Binbin , Zhong Jin , Jiu Yaming 
Published: August 2023

ABSTRACT
Emerging pathogen infections, such as Zika virus (ZIKV), pose an increasing threat to human health, but the role of mechanobiological attributes of host cells during ZIKV infection is largely unknown. Here, we reveal that ZIKV infection leads to increased contractility of host cells. Importantly, we investigated whether host cell contractility contributes to ZIKV infection efficacy, from both the intracellular and extracellular perspective. By performing drug perturbation and gene editing experiments, we confirmed that disruption of contractile actomyosin compromises ZIKV infection efficiency, viral genome replication and viral particle production. By culturing on compliant matrix, we further demonstrate that a softer substrate, leading to less contractility of host cells, compromises ZIKV infection, which resembles the effects of disrupting intracellular actomyosin organization. Together, our work provides evidence to support a positive correlation between host cell contractility and ZIKV infection efficacy, thus unveiling an unprecedented layer of interplay between ZIKV and the host cell.
De-Implementation of Low-Value Care for Women 70 Years of Age or Older with Low-Risk Breast Cancer During the COVID-19 Pandemic
Authors: Wang Ton , Weed Christina , Tseng Joshua , Chung Alice , Boyle Marissa K. , Amersi Farin , Jutla Jaswinder , Mirhadi Amin , Giuliano Armando E. 
Published: August 2023

Abstract
Background
Older women with early-stage estrogen receptor-positive (ER+) invasive breast cancer (IBC) are at risk for overtreatment. Guidelines allow for sentinel lymph node biopsy (SLNB) and radiotherapy omission after breast-conserving surgery (BCS) for women 70 years of age or older with T1, clinical node negativity (cN0), and ER+ IBC. The study objective was to evaluate radiotherapy and SLNB de-implementation in older women with low-risk IBC after the resource limitations of the COVID-19 pandemic.

Methods
An institutional database was analyzed to identify women 70 years of age or older who received BCS for IBC from 2012 to 2022. The patients were divided into two cohorts: (1) patients with low-risk IBC (pT1, cN0, and ER+/HER2–) who were eligible for radiotherapy and SLNB omission and (2) patients with high-risk IBC (pT2-T4, cN+, ER–, or HER2+) who were ineligible for therapy omission. Clinicopathologic variables in both cohorts were analyzed.

Results
The study enrolled 881 patients. For the patients with low-risk IBC, the annual rates of radiotherapy were stable from 2012 to 2019. However, radiotherapy utilization decreased significantly from 2020 to 2022 (58% in 2012 vs 36% in 2022; p = 0.04). In contrast, radiotherapy usage among the patients with high-risk IBC was stable from 2012 to 2022 (79% in 2012 vs 79% in 2022; p = 0.95). Among the patients with low-risk IBC, SLNB rates decreased from 86% in 2012 to 56% in 2022, but this trend predated those in 2020. The factors significantly associated with SLNB and receipt of radiotherapy among the patients with low-risk IBC were younger age, larger tumors, grade 3 disease, and involved nodal status (p < 0.01).

Conclusion
This study demonstrated appropriate and sustained de-escalation of radiotherapy in older women with low-risk IBC after the COVID-19 pandemic.
As we were and as we Should Be, Combined Exercise Training in Adults with Schizophrenia: CORTEX-SP Study Part I
Authors: Tous-Espelosin Mikel , Ruiz de Azua Sonia , Iriarte-Yoller Nagore , Sanchez Pedro M. , Elizagarate Edorta , Maldonado-Martín Sara 
Published: August 2023

ABSTRACT

Introduction
Given the health benefits and the role of exercise as an anti-inflammatory adjuvant program, this study aimed to determine the effectiveness of a combined exercise program on cardiorespiratory fitness (CRF), body composition, and biochemical levels in adults with schizophrenia (SZ) characterized at baseline as metabolically unhealthy overweight with low CRF.

Methods
Participants diagnosed with SZ (n = 112, 41.3 ± 10.4 yr, 28.7% women) were randomly assigned into a Treatment-As-Usual (TAU) control group (n = 53) or a supervised exercise group (n = 59, 3 days/week). Each combined exercise session consisted of both a low-volume high-intensity interval training (less than 10 min of high-intensity time per session) and a resistance circuit-training program. All variables were assessed pre-and post-intervention (20 weeks). For the assessment of CRF, a peak cardiopulmonary exercise test on a cycle ergometer was used.

Results
Following the intervention, participants from the exercise group (n = 51) showed increases in CRF (P < 0.001) through peak oxygen uptake (L·min-1; Δ = 17.6%; mL·kg.1·min-1, Δ = 19.6%) and the metabolic equivalent of task (Δ = 19%), with no significant changes (P > 0.05) in body composition and biochemical variables. However, the TAU group (n = 38) did not show any significant change in the study variables (P > 0.05). Between-group significant differences (P ≤ 0.05) were observed in CRF, first ventilatory threshold, and heart rate peak after the intervention period favoring the exercise group.

Conclusions
This study demonstrated that a supervised combined exercise program in people with SZ helps to maintain body composition values and improve CRF levels. This could lead to an important clinical change in the characterization from metabolically unhealthy overweight to a metabolically healthy overweight population. Hence, exercise should be considered a co-adjuvant program in the treatment of the SZ population.
Translating Basic Science to Clinical/Real-World Applications: Relevance for Exercise Science
Authors: Bloomfield Susan A. 
Published: August 2023

ABSTRACT
Among the thousands of American College of Sports Medicine members, many are basic science researchers. Although most are physiologists, cognitive/neuroscience specialists are also contributing to the literature with important new findings about the physical and mental health effects of regular exercise. Although experimental work in cell culture or isolated tissue models can rapidly identify potential cellular and molecular mechanisms, basic scientists must work with appropriate animal models to confirm that mechanisms identified in the highly controlled environment of the petri dish still pertain when the tissue of interest is also exposed to the multiple neural, endocrine, and paracrine signals that pertain to the intact living organism. One form of translational research this journal seeks to publish are intervention trials testing whether earlier results gleaned from appropriate animal models will “translate” to human physiology.
Exercise Is Medicine as a Vital Sign: Challenges and Opportunities
Authors: Bowen Pamela G. , Mankowski Robert T. , Harper Sara A. , Buford Thomas W. 
Published: August 2023

ABSTRACTExercise is widely recognized as a critical component of a healthy lifestyle by multiple scientific and regulatory bodies. Yet only 35% of adults in the United States even report engaging in regular physical activity. In response to this challenge, the Exercise Is Medicine (EIM) initiative encourages clinicians in all settings to regularly assess and counsel patients on physical activity in an attempt to reduce levels of inactivity. The purpose of this article is to highlight key challenges to the implementation of the EIM initiative to promote physical activity and to identify potential opportunities to overcome these challenges. EIM is a global health initiative that endorses “exercise” to be a “vital sign” in all clinical settings, specifically to assess and compare the patient’s physical activity level to national guidelines for physical activity and then promote discussions or referrals based on results. Since its 2007 launch, advocates of the EIM initiative have increased global awareness to promote the EIM mission of integrating and implementing physical activity into health care systems, local communities, and individuals’ daily lives. Physical activity is fundamental to the overall health of all people across all geographic locations and throughout the life span. Promoting physical activity as a healthy lifestyle change has specific challenges but necessary to decrease health disparities related to inactivity.
Evolução do Regime Federativa
Authors: Silva Carlos Medeiros 
Published: August 2023

1. Antecedentes históricos. 2. A Constituição do Império. O germe dafederação. 3. O manifesto de 1870, do Partido Republicano. 4. Triunfoda federação com a república. 5. Confronto com o modelo orteamericano.6. A Constituição de 1891. 7. Depoimentos eloqüentes. 8. As Constituições de 1934 e 1946. 9. A tendência à centralização e suas causas. 10. Exemplos de outros países. 11. Conclusão.
A exaustão da via administrativa como condição da ação
Authors: Azevedo Eurico de Andrade 
Published: August 2023

1. Conceito de ação. 2. As condições da ação. 3. O interesse de agir e a exaustão da via administrativa. 4. O princípio da inafastabilidade do controle jurisdicional. 5. A legislação positiva e a jurisprudência. 6. Conclusões.
Desistência de desapropriação de ações
Authors: Tácito Caio 
Published: August 2023

1. Constitucionalidade da desapropriação de ações. 2. Intervenção doEstado no domínio econômico: posição da empresa pública e dasociedade de economia mista. 3. Desapropriação como forma extintade concessão de serviço público. 4. Desistência de desapropriação:restauração do statu quo ante. 5. Impossibilidade de devolução decoisa diversa. 6. Imissão provisória na posse e incorporação da sociedade. 7. Eficácia da coisa julgada.
Desapropriação de ações - Desistência - Companhia Paulista de Estradas de Ferro
Authors: Meirelles Hely Lopes , Tácito Caio 
Published: August 2023

- Sustentação de decisão agravada pelo Dr. Juiz de Direito da 2º Vara dos Feitos da Fazenda Estadual de São Paulo.·• A decisão agravada está publicada no volume 154 desta revista, p. 156 e segs., assim como os pareceres de Caio Tácito e Hely Lopes Meirelles (mesmo volume, p. 239 e sega. e 248 e segs.). No volume n9 156, desta revista, figura o acórdão do Tribunal de Justiça do Estado de São Paulo no Agravo de Instrumento n9 70.934, que manteve a decisão agravada.
Empreitada - Correção monetária - Novação
Authors: Tácito Caio 
Published: August 2023

- Não importa novação a tolerancia do credor no recebimento da dívida.- O dies a quo para incidência da correção monetária é o dia estipulado para pagamento da fatura.- Inexiste compensação entre dívida vencida líquida e certa e alegada indenização por atraso na execução da obra.
A corrupção eleitoral no direito romano (as leges de ambitu)
Authors: Ferraz Manoel Martins de Figueiredo 
Published: August 2023

1. As leges de ambitu e a corrupção eleitoral em Roma. 2. As leiscontra o ambitus segundo Monunsen. 3-. A compra de votos e a manifestação de Plutarco. 4. As primeiras leis contra a corrupçãoeleitoral. 5. A corrupção eleitoral em Roma no relato de Ferrero.6. O principado de Augusto e a Lei Júlia de ambitu. 7. Os "divisores'e a compra de votos. 8. Os limites legais da campanha eleitoraldo candidato. 9. Classificação das magistraturas e condiçõespara seu exercício. 10. Características das magistraturas romanas.11. Apresentação e registro dos candidatos. 12. A importância doscomitia centuriata. 13. O processo de votação. 14. Apuração dosvotos e proclamação dos eleitos. 15. As interrupções durante o processode votação. 16. As leis de ambitu e as penalidades aplicadas.17. Conclusão.
A estabilidade na disposição transitória da Constituição
Authors: Desconhecido Autor 
Published: August 2023

- Constituições de 1946 e 1967. Constituição de 1988. Conceito tradicional de e:Jtabilidade. O pressuposto da efetividade. Doutrina e jurisprudência. Alcance da norma transitória vigente. Extensão a funcionários e empregados públicos. Exigência de efetivo exercício. Estabilidade sem efetividade. Efeitos em relação à exoneração ou dispema, demissão e extinção do cargo ou emprego. Pessoal das fundações públicas. Pessoal temporário. Concurso exigível.
Concessão de transporte aéreo - Intervenção no serviço - Fundação
Authors: Tácito Caio 
Published: August 2023

- Intervenção decretada em concessionária de transporte aéreo atinge oserviço, não se estendendo à economia da empresa. Fundação instituída pela empresa concessionária não está alcançada pelo ato de intervenção no serviço.
As medidas provisórias na Constituição de 1988
Authors: Tácito Caio 
Published: August 2023

1. introdução. 2. Antecedentes: decreto-Iei. 3. Efeitos da conversão.4. Requisitos de relevância e urgência. 5. Inspiração no modelo italiano.6. Revogação da lei anterior. 7. Direito de emenda. 8. Renovaçt1o da medida provisória.
As sociedades de economia mista e as empresas estatais perante a Constituição de 1988
Authors: Penteado Mauro Rodrigues 
Published: August 2023

1. Introdução. 2. Breve escorço histórico. 3. Sociedades de economiamista e subsidiárias ou empresas controladas por outras sociedadesde economia mista: exigência de autorização legal para suacriação. 4. Subsidiárias ou controladas de sociedades de economiamista, criadas à mlngua de autorização legal espec(fica. Regime jurfdico.5. Diretriz atualmente dominante no âmbito da administraçãofederal, Tribunal de Contas da União, doutrina e jurisprudência.6. Conclusão: o controle e a gestão de empresas com participaçãomajoritária do Estado, que não são sociedades de economia mista.
Poder discricionário no direito administrativo brasileiro
Authors: Silva Almiro do Couto e 
Published: August 2023

1. O Estado e a vinculação à Lei. 2. Conceito do poder discricionário. 3. Poder discricionário e conceitos jurídicos indeterminados. 4. Exercício e limites do poder discricionário. 5. Controle judicial do poder discricionário.
Espécies de tributos
Authors: Oliveira José Marcos Domingues de 
Published: August 2023

1. Critérios de classificação dos tributos: competência, finalidade, fato gerador e capacidade contributiva. 2. Empréstimos compulsórios e contribuições parafiscais; conceituação de tributos. 3. A Constituição de 1988 e a reforma do Código Tributário Nacional. 4.Conclusões.
A administração Pública nas Constituição de 1988
Authors: Oliveira Fernando Andrade de 
Published: August 2023

1.Introdução. 2. A administração pública sob o aspecto orgânico ou subjetivo. 3. Administração direta e indireta. 4. Autarquia e fundação pública. 5. Administração pública em sentido substancial, material ou objetivo. 6. Administração pública de fomento. 7. Políciaadministrativa. 8. Serviços públicos.
Eficácia das decisões dos tribunais de contas
Authors: Custódio Antonio Joaquim Ferreira 
Published: August 2023

1 - Natureza jurídica dos tribunais de contas. 2 - A Constituição de 1988 e os tribunais de contas. 3 - Eficácia executiva de decisões sobre débito ou multa. 4 - Conclusão.
Direito à honra - Direito constitucional - Dano moral
Authors: Direito Carlos Alberto Menezes 
Published: August 2023

- Indenização por dano moral. Direitos da personalidade.- I. Os direitos da personalidade estão agrupados em direitos à integridade física (direito à vida; direito sobre o próprio corpo; e direito ao cadáver) e direitos à integridade moral (direito à honra; direito à liberdade, direito ao recato; direito à imagem; direito ao nome; direito moral do autor). A Constituição Federal de 1988 agasalhou nos incisos V e X do art. 5.° os direitos subjetivos privados relativos à integridade moral.- II. Dano moral. Lição de Aguiar Dias: o dano moral é o efeito não patrimonial da lesão de direito e não a própria lesão abstratamente considerada. Lição de Savatier: dano moral é todo sofrimento humano que não é causado por uma perda pecuniária. Lição de Pontes de Miranda: nos danos morais a esfera ética da pessoa é que é ofendida: o dano não patrimonial é o que, s6 atingindo o devedor como ser humano, não lhe atinge o patrimônio.- III. O ser humano tem uma esfera de valores próprios que são postos em sua conduta não apenas em relação ao Estado, mas, também, na convivência com os seus semelhantes. Respeitam-se, por isso mesmo, não apenas aqueles direitos que repercutem no seu patrimônio material, mas aqueles direitos relativos aos seus valores pessoais, que repercutem nos seus sentimentos. Não é mais possível ignorar esse cenário em uma sociedade que se tornou invasora porque reduziu distâncias, tornando-se pequena, e, por isso, poderosa na promiscuidade que propicia. Daí ser desnecessário enfatizar as amea- ças à vida privada que nasceram no curso da expansão e desenvolvimento dos meios de comunicação de massa.- IV. Nenhum homem médio poderia espancar os seus mais íntimos sentimentos de medo e frustração, de indignação e revolta de dor e mágoa, diante da divulgação de seu nome associado a uma doença incurável, desafiadora dos progressos da ciência e que tantos desesperos tem causado à humanidade.- V. O art. 5.°, X, da Constituição Federal assegura ao ser humano o direito de obstar a intromissão na sua vida privada. Não é lícito aos meios de comunicação de massa tornar pública a doença de quem quer que seja - ainda mais quando a notícia é baseada apenas em boatos -, pois tal informação está na esfera ética da pessoa humana, dizendo respeito à sua intimidade, à sua vida privada. Só o próprio paciente pode autorizar a divulgação de notícia sobre a sua saúde.- VI. A reparação do dano moral deve adotar a técnica do quantum fixo.Apelo provido.