bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Characterization of Glaesserella parasuis Strains Circulating in North American Swine Production Systems
Authors: Mugabi Robert , Silva Ana Paula S. Poeta , Hu Xiao , Gottschalk Marcelo , Aragon Virginia , Macedo Nubia R. , Sahin Orhan , Harms Perry , Main Rodger , Tucker Alexander W. , Li Ganwu , Clavijo Maria J. 
Published: March 2023

Abstract
Background
Glaesserella parasuis is the causative agent of Glässer’s disease in pigs. Serotyping is the most common method used to type G. parasuis isolates. However, the high number of non-typables (NT) and low discriminatory power make serotyping problematic. In this study, 218 field clinical isolates and 15 G. parasuis reference strains were whole-genome sequenced (WGS). Multilocus sequence types (MLST), serotypes, core-genome phylogeny, antimicrobial resistance (AMR) genes, and putative virulence genes were determined.
Results
In silico WGS serotyping revealed 11 of 15 serotypes. The most frequently detected serotypes were 7, 13, 4, and 2. MLST identified 72 sequence types (STs) (66 novel). The most predominant ST was novel ST454. At least one group 1 vtaA virulence gene was observed in all isolates, except for serotype 8 (ST299 and ST406), 15 (ST408 and ST552) and NT (ST448). A few group 1 vtaA genes were significantly associated with certain serotypes or STs. Core-genome phylogeny depicted 3 primary lineages (LI, LII, and LIII), with LIIIA sublineage isolates lacking all vtaA genes.
Conclusion
This study showed the use of WGS to type G. parasuis isolates and can be considered an alternative to the more labor-intensive and traditional serotyping and standard MLST. Core-genome phylogeny provided the best strain discrimination. The presence of other putative virulence factors and AMR genes was also explored. These findings will lead to a better understanding of the molecular epidemiology and virulence in G. parasuis that can be applied to the future development of diagnostic tools, autogenous vaccines, evaluation of antibiotic use, prevention, and disease control.
Perisellar tumor coexisting with unruptured cerebral aneurysm: single-stage or staged surgery?
Authors: Wan Xi Chen , Chen Yu Xing , Wu Jie , Chen Shan Si , Tang Bin , Hong Tao , Wu Xiao 
Published: March 2023

Abstract
Objective
The coexistence of perisellar tumors and intracranial aneurysms was previously considered a rare phenomenon. In this study, we introduce our experience with surgical strategies for the treatment of such coexisting pathologies.
Methods
This retrospective study reviews the medical records and intraoperative videos of patients with coexisting pathologies of perisellar tumors and aneurysms from Nov 2017 to Oct 2022.
Results
Our study involved 20 patients, including 9 males and 11 females, with an average age of 53 years. All tumors were completely resected through the endoscopic endonasal approach (EEA) with the exception of 2 asymptomatic pituitary microadenomas with aneurysms in the internal carotid artery (ICA) C1 and C4 segments; for the latter, conservative management was chosen. Thirteen patients chose tumor resection first through the EEA with embolization or aneurysm conservation. Three patients selected the single-stage EEA, including one with a type of MP trigeminal schwannoma with an anterior communicating aneurysm, one with a pleomorphic xanthoastrocytoma with a left ICA paraclinoid aneurysm, and one with a recurrent pituitary adenoma with a right ICA paraclinoid aneurysm. In addition, there were 2 cases in which daughter aneurysms formed after embolization and before tumor resection. Of those, one patient who chose to treat the aneurysm conservatively experienced aneurysm rupture during the follow-up period. No CSF rhinorrhea, severe intracranial infection or surgical-related hemorrhage were found in any of the patients.
Conclusion
Staged surgery or conservative treatment for aneurysms can be considered a safe and effective strategy for the treatment of coexisting pathologies. It is necessary to decide the sequence of staged surgery according to whether the tumor causes obvious neurological symptoms or aneurysms with small processes. However, in very selected cases, the single-stage EEA can be used as part of a comprehensive treatment for such coexisting pathologies.
Ameliorating Prediabetes Symptoms via Aerobic Exercise Combined with Green Coffee and Chlorogenic Acid Intake by Targeting MondoA-TXNIP/ARRDC4-GLUT4 Axis inHigh Fat Diet-Induced Prediabetic Mice
Authors: Sadeghian-Renani Nahid , Marandi Sayyed Mohammad , Reisi Jalil , Safaeinejad Zahra , Nasr-Esfahani Mohammad Hossein , Marandi Sayed Mohammad 
Published: March 2023

Abstract
Background and Aim:
Type 2 diabetes (T2D) begins with prediabetes (PD), whose primary cause is insulin resistance (IR) in the muscle, liver, and adipose tissues, followed by hyperinsulinemia induced by beta (β)-cells and their destruction. As metabolic balance and homeostasis in the human body heavily depend on skeletal muscles, metabolic syndrome (MetS), hepatic steatosis (HS), and T2D may all occur by skeletal muscle failure. Improving understanding of the molecular mechanisms of muscle energy homeostasis and IR may accordingly help in discovering novel methods for combating metabolic disorders, like T2D. In this way, MondoA, an essential glucose-responsive transcription factor regulated by nutrients, such as glucose 6-phosphate (G6P), can reduce the increased amount of glucose inside the cell and prevent more glucose from entering the cell by amplifying the expression of two insulin pathway suppressors, viz, thioredoxin-interacting protein (TXNIP) and arrestin domain containing protein 4 (ARRDC4), under normal conditions. Against this background, the main objective of the present study was to examine the effects of aerobic exercise (Exe) alone and in combination with green coffee (GC) and chlorogenic acid (CGA) intake on the MondoA activity and the expression of its target genes in the skeletal muscles of a high-fat diet (HFD)-induced PD mouse model.
Materials and Methods
To develop PD, the C57 black 6 (C57BL/6) mice recruited in this study received an HFD for 12 weeks. Upon confirming the PD induction, the animals were given Exe, GC, CGA, or their combination for 10 weeks. Afterward, the metabolic data, i.e., fasting blood sugar (FBS), glucose tolerance test (GTT), the homeostatic model assessment for IR (HOMA-IR) index, plasma insulin levels, together with the cellular localization of MondoA and glucose transporter type 4 (GLUT4), as well as the TXNIP and ARRDC4 expression in the myocytes from various groups were evaluated.
Results
After administering the treatments in this study, the results established a downward trend in the TXNIP and ARRDC4 expression, a drop in the presence of MondoA in the cell nucleus, and a rise in GLUT4 in the muscle cell membrane.
Conclusion
By reducing the MondoA activity in the cell nucleus and expanding the presence of GLUT4 in the cell membrane, Aerobic exercise alone and in combination with green coffee and chlorogenic acid intake may be thus useful in lowering blood sugar and improving PD conditions in mice.
Multi-metal Nanozyme properties for colorimetric peroxidase reaction: Overview of an applicable method validation
Authors: Ravannakhjavani Fatemeh , Dehghan Somayeh Farhang , Panahi Davod , Moradpoor Zahra , Zendehdel Rezvan 
Published: March 2023

Abstract
Displacement of enzyme mimic nanomaterials has been developed due to disadvantages of natural enzymes. Peroxidases reaction could be used for H2O2 colorimetric evaluation in different media. We aim to develop a new method based on the best catalytic activity of metal Nanozyms. A method for H2O2 detection was carried out by TMB peroxidation of metal nanozymes. In this way peroxidase activity of Ag-nano zeolite Y, Cu-nano zeolite Y, Zn-nano zeolite Y and Ag,Cu,Zn -nano zeolite Y was evaluated by Central Composite Design. Total of 28.42 % from three metals was exchanged in multi metal nano zeolite Y (mMy) with higher peroxidase- like activity. Nanozyme Km and Vm were evaluated 0.076 and 8.76×108, respectively which produced fast and sensitive catalytic reaction.H2O2 method detection carried out by LOD level of 0.12 ppm, and recovery parameters of 99%, in the linear range of 0.5-50 ppm.The mMy nanozyme was validated for H2O2 colorimetric detection. In conclusion an applicable way as a fast, sensitive, accurate and reproducible method has been suggested for occupational exposure monitoring. Performance of validated method is higher than international OSHAVI-6 method.
A fuzzy approach to provide different information retrieval results for the same query
Authors: Chkiwa Mounira , Achour Fatma 
Published: March 2023

Abstract
In this paper, we present a feature of our information system which provide different results for users even they submit the same query. This differentiation is due to a main input taken in consideration: the user’s age. Besides of this input, results differentiation is based on the centralized document valorization defined in this paper. A centralized Web document’s content is mainly concise and focusing on the user interests materialized by keywords query. While an information retrieval process, web documents are ranked according to their relevance to a user query. This ranking is changed afterward by the valorization of centralized Web documents. The valorization value is based on the document features and the user age. This value could be positive or negative implying thus the enhancement of the ranking of the concerned document or its rank decrease. Hence, the users of our information retrieval system can get different results according to their ages even submitting the same query.
Graphene nanoplatelets/hydroxyapatite in PMMA composites for enhanced electrical properties
Authors: Abdullah Ahmed Q. , Ali Nadia A. , Hussein Seenaa I. 
Published: March 2023

Abstract
In this article, the casting method was used to prepare PMMA/hydroxyapatite nanocomposite films incorporated with contents graphene nanopalates (Gnp) of nano-size with combined loadings ranging from 0.5 to 1.5 weight %. The nanocomposite was characterized using Fourier transforms infrared (FTIR) spectroscopy, and. Scanning Electron Microscopy (SEM) analysis of the prepared sample, thermal conductivity, and the electrical properties at (1-10MHz) of the samples. The results for FT-IR, and SEM showed that the resulting material had a low agglomerated state, with multiple wrinkles of graphene flakes in the matrix of the PMMA. The thermal conductivity increased and improvement between (0.05–1.82) w/m.k and the electrical properties of nanocomposites revealed a significant improvement with increasing the amount of graphene also that exponent s between (1.25 < s < 1.35). The values of real part ɛ' and imaginary part ɛ’’ were reduced at the lower frequency and became constant at high frequency attributed to the relaxation time. The values of ε″ are high at low frequencies and it is decrease with increase frequencies due to polarization effects or to the dipoles not begins to follow the field variation at higher frequencies. The increase in tan δ with increase in Gnp content due to interfacial polarization mechanism occurred in the films corresponding to frequencies.
Effects of Parents' Health on Children's Educational Attainment
Authors: Ahn Joonhong 
Published: March 2023

Abstract
This paper examines the effects of parental health on children's educational attainment. Parental illness changes parenting quality both by affecting family wealth and in other ways that influence children's labor market outcomes by creating an educational environment and extra educational opportunities in childhood. Longitudinal data from the Korean Labor Income Panel Survey, for the period 1998 - 2018, enables me to examine parental illness effects in the early childhood and adolescent periods on children's ultimate educational achievement. Empirical application in this paper considers the effects of both unexpected and chronic health problems on children's human capital. We find that a parent's health problems, especially in the early childhood period, are strongly associated with a child's education.
JEL Codes: J62, I14, I24, I26
Prevalence of intestinal stoma complications and associated factors among children in Uganda, a cross sectional study
Authors: Kimbugwe Godfrey , Kakembo Nasser , Kisa Phyllis , Okeny Paul , Sekabira John 
Published: March 2023

Abstract
Purpose: Intestinal stomas are a common procedure performed in children. They carry a high complication rate which affects the child, family and hospital. This study was aimed at documenting these complications and their associated factors.
Methods: This was a cross-sectional study carried out at the paediatric surgery unit of Mulago Hospital which is the main referral hospital in Uganda over a period of one and a half months. It included 131 children whose caretakers were interviewed and children physically assessed for presence of intestinal stoma complications.
Results: The mean age was 2 years (SD±1) with a male to female ratio of 1.3:1. Anorectal malformation (ARM) (77/131) was the most common indication for stoma formation followed by Hirschsprungs disease (25/131). Sigmoid colon was the most exteriorised part (104/131) followed by ileum (27/131). Children with intestinal stomas who developed complications were 83/131 with peristomal dermatitis being the most common complication (54/131) followed by stoma prolapse (5/131). The factors associated with intestinal stoma complications were stoma placement after the age of 12 months (OR 10.7, CI 1.47-17.62, p-value 0.019) and exteriolization of the ileum (CI 0-0.66, p-value 0.028).
Conclusion: The prevalence of intestinal stoma complications is high in Uganda with stoma placement after the age of one year and having an ileostomy as the associated factors. Sigmoid divided colostomies and prioritization of children with ileostomies for stoma reversal will help to significantly reduce intestinal stoma complications.
Low-temperature Small-angle Electron-electron Scattering Rate in Fermi Metals
Authors: Arulsamy Andrew Das 
Published: March 2023

Abstract
Low-temperature elementary conductivity formula in Fermi metals is reviewed starting from Ohm’s law. This provides the background needed to understand why and how the equations exploited are complicated due to effective mass effect and complex scattering rate even in the presence of small-angle electron-electron scattering at low temperatures. Using the mathematical conditions and physical arguments exploited to derive the Drude conductivity formula, we arrive at our main result—the analytic scattering rate formula at low temperatures that gives rise to the famous T2 dependence without any ad hoc constants. Our derivation formally proves that the formula, 1/τ = (A/ℏ)(kBT)2/EF first guessed by Ashcroft and Mermin to be correct where A = Nimpurity/4π2 and Nimpurity is the number of impurities (or scattering centers) present in a given sample. We also highlight the discovery of a new fundamental physical constant, λArulsamy = [3ℏ2(4πε0)2]/[mele4] that associates quantum mechanical energy with that of thermal energy, and is also related to Rydberg constant.
PACS: 72.10.Bg; 72.10.-d
Distribution and relationship of antibiotics, heavy metals and resistance genes in the Hanjiang Reservoir, Shiyan, China
Authors: Jing LI , Li Lijuan , Li Qin , Fang Wen , Sun Yonghao , Lu Yu , Wang Jing , Zhu Yanrong , Zhang Yao 
Published: March 2023

Abstract
The Hanjiang Reservoir is an important water source for the middle route of China's South-to-North Water Diversion Project. The problems of water and soil quality in the Hanjiang reservoir have enormous biological and environmental impacts, resistant genetic contamination has emerged, but only few studies are concerned the correlation between heavy metals and metal resistance genes (MRGs). In this study, 8 antibiotics and 19 heavy metals were analyzed, the results showed that the highest antibiotic content was tetracycline, with mean concentrations of 43.2 µg/kg and 0.0221 µg/L. Mn was the highest heavy metal in soil with a content of 1,408.28 µg/kg, and in water was Zn with a content of 10.61 µg/L. We found that the most abundant antibiotic resistance genes (ARGs) and MRGs in the study area were bacA and arsT genes, coding for resistance mechanisms to bacitracin and arsenic, respectively. The data showed that heavy metals had a greater impact on antibiotic genes than antibiotics, and antagonism between resistance genes was more significant. This work expands our understanding of the correlations of antibiotics, heavy metals, and resistance genes in the Hanjiang reservoir, indicating that more attention should be paid to the effects of resistance genes and the quality of water.
Human clostridia improve visceral hypersensitivity in stressed mice through upregulation of 5-hydroxytryptamine 4
Authors: Xiao Yaodong , Miao Bei , Li Bowen , Li Li , Fei Sujuan , Zhao Yanchao 
Published: March 2023

Abstract
Background
Clostridium improves intestinal motility by modulating intestinal 5-hydroxytryptamine (5-HT) levels. However, there are few studies on human clostridia.
Aims
To evaluate whether human clostridia improves visceral hypersensitivity through the 5-HT4 pathway.
Methods
An irritable bowel syndrome (IBS) model was established using water avoidance stress (WAS) mice. WAS mice were treated with saline, human clostridial solution (enriched with Clostridium clusters IV ), prucalopride, PCPA, or RS39604 for 1 week, and group differences in body weight, fecal traits, abdominal withdrawal reflex (AWR), and electromyography results were evaluated. Group differences in SLC6A4, TPH1, 5-HT4, PKA, CREB, and pCREB expression in the distal colon and hippocampus and in the number of 5-HT4-expressing intestinal cells were also evaluated. Fecal short-chain fatty acid levels were measured.
Results
WAS mice showed slower body weight gain, lower fecal water content and Bristol score, and higher visceral sensitivity than the normal group. 5-HT4, PKA, CREB, and pCREB levels and the number of 5-HT4-expressing intestinal cells were lower in the distal colon and hippocampus in WAS mice than in normal mice. After 1 week, human clostridia treatment was superior to other treatments, and the levels of 5-HT4 signaling protein in the intestine and hippocampus and 5-HT4-expressing intestinal cells increased. The TPH1 antagonist PCPA or the 5-HT4 receptor antagonist RS39604 could interfere with the therapeutic effect of the bacterial solution, and human clostridia elevated fecal isovaleric acid levels.
Conclusion
Human clostridia improved visceral hypersensitivity by upregulating 5-HT4 signaling protein expression in the distal colon and hippocampus, demonstrating its therapeutic potential.
Experience of Psychosocial Rehabilitation; Perspectives of Depressed Adolescents
Authors: Kodzo Lalit Dzifa , Danso Nana Ama Asi , Budu Jemima Twumwaa , Kafle Baral Akriti , Zhang Ruixing 
Published: March 2023

Abstract
Given the multifaceted character of depression and its related symptoms, an adolescent living with it is at increased risk for a wide range of adverse events. This research aimed to understand and characterize the psychosocial rehabilitation experiences of depressed adolescent participants in the Greater Accra Region of Ghana. A cross-sectional semi-structured interview design influenced by an interpretive phenomenological analysis (IPA) technique was adopted. A nonprobability, purposeful approach to sampling was employed to recruit twenty-one adolescents (6 males, 15 females) from the community diagnosed with depression, discharged from admission for a month, and undergoing psychosocial rehabilitation. Using separate interviews, we gathered data and analyzed using interpretive phenomenological analysis to produce themes and sub-themes. These were presented with the participants' direct quotations. We discovered that the perspectives of adolescents' psychosocial rehabilitation experience include hopelessness and suicide ideation, coping difficulties, undesirable attitudes from support networks, challenges related to school, and isolation. Participants suggested appropriate therapeutic environments, encouraging support systems, and the media's role in preventing and treating depression among young people as rehabilitation approaches that could assist adolescents realize their “hoped-for” selves despite the diagnosis with depression. These results shed light on the tragic realities faced by depressed adolescents, confirming the urgent necessity to focus on their plights to aid in their rehabilitation and develop their coping strategies for a better life.
Discovery, mechanisms, and engineering of the missing apiosylation step in the biosynthesis of flavonoid apiosides in Leguminosae plants
Authors: Wang Hao-Tian , Wang Zi-Long , Chen Kuan , Yao Ming-Ju , Zhang Meng , Wang Rong-Shen , Zhang Jia-He , Ågren Hans , Li Fu-Dong , Li Jun-Hao , Qiao Xue , Ye Min 
Published: March 2023

Abstract
Apiose is a natural pentose containing an unusual branched-chair structure. Apiosides are bioactive natural products widely present in the plant kingdom. However, the essential apiosylation reaction in the biosynthetic pathways of apiosides has been missing. In this work, we report the first apiosyltransferase GuApiGT, which from medicinal plant Glycyrrhiza uralensis. GuApiGT could efficiently catalyze 2″-O-apiosylation of flavonoid glycosides, and exhibits strict sugar donor selectivity towards UDP-apiose. We solved the crystal structure of GuApiGT, determined a key sugar-binding motif (RLGSDH) through structural analysis and theoretical calculations, and obtained mutants with altered sugar selectivity through protein engineering. Based on the motif, we discovered 121 candidate apiosyltransferase genes from Leguminosae plants, and identified the functions of 4 enzymes. Moreover, we introduced GuApiGT and its upstream genes into Nicotiana benthamiana, and completed de novo biosynthesis of a series of flavonoid apiosides, including the anti-tussive compound liquiritin apioside at a yield of 5.46 mg/g (DW).
Microwave electrometry with bichromatic electromagnetically induced transparency in Rydberg atoms
Authors: Han Mingzhi , Hao He , Song Xiaoyun , Yin Zheng , Parniak Michal , Zeng Qingtian , Peng Yandong 
Published: March 2023

Abstract
A scheme for measuring microwave (MW) electric (E) fields is proposed based on bichromatic electromagnetically induced transparency (EIT) in Rydberg atoms. A bichromatic control field drives the excited state transition, whose absorption shows three EIT windows. When a MW field drives the Rydberg transition, the EIT windows split and six transmission peaks appear. It is interesting to find that the peak-to-peak distance of transmission spectrum is sensitive to the MW field strength, which can be used to measure MW E-field. Simulation results show that the measurement accuracy could be increased by about 4 times, and the minimum detectable strength of the MW E-field may be improved by about 3 times compared with the common EIT scheme. After the Doppler averaging, the minimum detectable MW E-field strength is about 5 times larger than that without Doppler effect. Also, we investigate other effects on the sensitivity of the system.