bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relevance of HLA-DP/DQ and INF-λ4 Polymorphisms to COVID-19 Outcomes
Authors: Ghazy Amany A. , Alrasheedi Abdullah N. , Elashri Mohammed , Moussa Hany Hussein , Rashwan Eman K. , Amer Ibrahim , El Sharawy Shimaa , Elgamal Shimaa , Tawfik Salwa , Abdelnasser Mohamed , Elsheredy Amel 
Published: January 2023

Background: Single nucleotide polymorphisms provide information on individuals’ potential reactions to environmental factors, infections, diseases, as well as various therapies. A study on SNPs that influence SARS-CoV-2 susceptibility and severity may provide a predictive tool for COVID-19 outcomes and improve the customized coronavirus treatment.Aim: To evaluate the role of human leukocyte antigens DP/DQ and IFNλ4 polymorphisms on COVID-19 outcomes among Egyptian patients.Participants and Methods: The study involved 80 patients with severe COVID-19, 80 patients with mild COVID-19, and 80 non-infected healthy volunteers. Genotyping and allelic discrimination of HLA-DPrs3077 (G/A), HLA-DQrs7453920 (A/G), and IFNλ4 rs73555604 (C/T) SNPs were performed using real-time PCR.Results: Ages were 47.9 ± 8, 44.1 ± 12.1, and 45.8 ± 10 years in severe, mild and non-infected persons. There was a statistically significant association between severe COVID-19 and male gender (p = 0.002). A statistically significant increase in the frequency of HLA-DPrs3077G, HLA-DQrs7453920A, and IFNλ4rs73555604C alleles among severe COVID-19 patients when compared with other groups (p &lt; 0.001). Coexistence of these alleles in the same individual increases the susceptibility to severe COVID-19 by many folds (p &lt; 0.001). Univariate and multivariate logistic regression analysis for the studied parameters showed that old age, male gender, non-vaccination, HLA-DQ rs7453920AG+AA, HLA-DPrs3077GA+GG, and IFNλ4rs73555604CT+CC genotypes are independent risk factors for severe COVID-19 among Egyptian patients.Conclusion: HLA-DQ rs7453920A, HLA-DPrs3077G, and IFNλ4rs73555604C alleles could be used as markers of COVID-19 severity.
Treatment of engineering waste slurries by microbially induced struvite precipitation mechanisms
Authors: He Yuhan , Liu Shiyu , Shen Gangqiang , Pan Muzhi , Cai Yanyan , Yu Jin 
Published: January 2023

With societal development, the growing scale of engineering construction, and the increase in environmental protection requirements, the necessity of engineering waste mud disposal is becoming increasingly prominent. In this study, microbially induced struvite precipitation (MISP) was introduced to treat engineering waste mud. The study mainly focused on: i) the optimal mineralization scheme for microbially induced struvite precipitation, ii) the feasibility of the process and the effect of reaction parameters on treating engineering waste mud with microbially induced struvite precipitation, and iii) the mechanism of microbially induced struvite precipitation in treating engineering waste mud. The results showed that the waste mud could be well treated with 8.36×106 cell⋅mL−1 bacteria, 10 mM urea, 20 mM phosphate buffer, and 25 mM MgCl2 at pH 7. The kaolin suspension could be effectively flocculated. The flocculation rate reached approximately 87.2% under the optimum mineralization conditions. The flocculation effect was mainly affected by the concentrations of reactants and heavy metals and the suspension pH. The X-ray diffraction (XRD) patterns showed a strong struvite (MAP) diffraction peak. Scanning electron microscopy (SEM) images indicated that under the optimal mineralization conditions, the crystals were large and showed prismatic shapes tilted at both ends with adhered kaolin particles. In summary, this manuscript provides an effective way to treat engineering waste mud, and the findings should have a positive effect on enhancing soil fertility and preventing secondary pollution.
Development and validation of nomogram models to discriminate between acute aortic syndromes and non-ST-elevation myocardial infarction during troponin-blind period
Authors: Tong Fei , Wang Yue , Sun Zhijun 
Published: January 2023

BackgroundBlood-test-based methods of distinguishing between acute aortic syndromes (AASs) and non-ST-elevation myocardial infarction (NSTEMI) during the troponin-blind period of &lt;2–3 h of symptom onset have not been studied previously. We aimed to explore whether routine biomarkers might facilitate differential diagnosis.MethodsData were retrospectively collected from 178 patients with AASs and 460 patients with NSTEMI within 3 h of onset. Differential risk factors related to AASs were identified by univariate and multivariate logistic regression analyses for patients with onset &lt;2 h and onset ≥2 h, respectively, in the cardiac troponin (cTn) cohort. Nomograms were established in the cTn cohort as a training set and validated in the high-sensitivity cTn cohort. To assess the utility of the models in clinical practice, decision curve analyses were performed.ResultsD-dimer, fibrinogen, and age were identified as differential risk factors for AASs with the onset of &lt;2 h. D-dimer at an optimal cutoff level of 281 ng/mL for AASs had a sensitivity of 86.4% and a specificity of 91.3%. A nomogram was developed and validated with areas under the curve (AUC) of 0.934 (95% CI: 0.880–0.988) and 0.952 (95% CI: 0.874–1.000), respectively. D-dimer, neutrophil, bilirubin, and platelet were the differential risk factors for AASs with the onset of ≥2 h. D-dimer at an optimal cutoff level of 385 ng/mL has a sensitivity of 91.8% and a specificity of 91.3%. The AUC of the second nomogram in the training set and the validation set were 0.965 (95% CI: 0.942–0.988) and 0.974 (95% CI: 0.944–1.000), respectively.ConclusionTime-dependent quality of D-dimer should be considered for discriminating AASs from NSTEMI. Both nomogram models may have a clinical utility for evaluating the probability of AASs.
A mixed integer linear programming model for minimum backbone grid
Authors: Mei Wenwen , Sun Zhiyuan , He Yuanjian , Liu Mosi , Gong Xianfu , Li Peijie 
Published: January 2023

Developing a minimum backbone grid in the power system planning is beneficial to improve the power system’s resilience. To obtain a minimum backbone grid, a mixed integer linear programming (MILP) model with network connectivity constraints for a minimum backbone grid is proposed. In the model, some constraints are presented to consider the practical application requirements. Especially, to avoid islands in the minimum backbone grid, a set of linear constraints based on single-commodity flow formulations is proposed to ensure connectivity of the backbone grid. The simulations on the IEEE-39 bus system and the French 1888 bus system show that the proposed model can be solved with higher computational efficiency in only about 30 min for such a large system and the minimum backbone grid has a small scale only 52% of the original grid. Compared with the improved fireworks method, the minimum backbone grid from the proposed method has fewer lines and generators.
Pakistan’s water resource management: Ensuring water security for sustainable development
Authors: Ishaque Waseem , Mukhtar Mudassir , Tanvir Rida 
Published: January 2023

Pakistan is blessed with abundant water resources but facing a critical water shortage due to a lack of storage facilities, poor water governance, and the adverse impacts of climate change. The burgeoning population at present 225 million, which is expected to cross 250 million by 2025 is creating an additional burden on water resources, as per capita availability is depleting sharply from 5,000 m3 in 1951 to 1,100 m3 in 2005, which is expected to fall to 800 m3 by 2025. Pakistan’s water demand is increasing at an average yearly rate of 10% which in terms of the area will increase to 338 km3 by 2025, against the availability of 240–258 km3. Out of the available surface water, approximately 74% is extracted, while 83% of the groundwater is used for agricultural and other uses, which is an exceptionally high ratio for a water-scarce country like Pakistan. The adverse impacts of climate change are also causing extreme weather conditions, resulting in drought and floods almost every year. Inadequate water reservoirs, water wastage in agriculture and households, negligible wastewater recycling, and contamination of drinking water are causing health risks that have turned serious national security challenges for Pakistan. This research article argues that surface and subsurface water in Pakistan is depleting sharply, and if not addressed in time, has the potential of becoming the biggest national security problem. The article investigates the available water status in Pakistan covering surface and subsurface resources in detail, including reservoirs for storage, and sectoral utilization, to draw conclusions on water availability and usage requirement. The policy recommendations highlight the effective integration of smart technology in addressing multidimensional water issues and efficient water governance for ensuring water security for the sustainable development of Pakistan.
Avian strains of emerging pathogen Escherichia fergusonii are phylogenetically diverse and harbor the greatest AMR dissemination potential among different sources: Comparative genomic evidence
Authors: Srinivas Kandhan , Ghatak Sandeep , Pyngrope Daniel Aibor , Angappan Madesh , Milton Arockiasamy Arun Prince , Das Samir , Lyngdoh Vanita , Lamare John Pynhun , Prasad Mosuri Chendu Bharat , Sen Arnab 
Published: January 2023

IntroductionEscherichia fergusonii is regarded as an emerging pathogen with zoonotic potential. In the current study, we undertook source-wise comparative genomic analyses (resistome, virulome, mobilome and pangenome) to understand the antimicrobial resistance, virulence, mobile genetic elements and phylogenetic diversity of E. fergusonii.MethodsSix E. fergusonii strains (5 multidrug resistant strains and 1 biofilm former) were isolated from poultry (duck faeces and retail chicken samples). Following confirmation by phenotypic and molecular methods, the isolates were further characterized and their genomes were sequenced. Comparative resisto-virulo-mobilome analyses and pangenomics were performed for E. fergusonii genomes, while including 125 other E. fergusonii genomes available from NCBI database.Results and discussionAvian and porcine strains of E. fergusonii were found to carry significantly higher number of antimicrobial resistance genes (p &lt; 0.05) and mobile genetic elements (plasmids, transposons and integrons) (p &lt; 0.05), while the pathogenic potential of bovine strains was significantly higher compared to other strains (p &lt; 0.05). Pan-genome development trends indicated open pan-genome for all strains (0 &lt; γ &lt; 1). Genomic diversity of avian strains was found to be greater than that from other sources. Phylogenetic analysis revealed close clustering among isolates of similar isolation source and geographical location. Indian isolates of E. fergusonii clustered closely with those from Chinese and a singleton Australian isolate. Overall, being the first pangenomic study on E. fergusonii, our analysis provided important cues on genomic features of the emerging pathogen E. fergusonii while highlighting the potential role of avian strains in dissemination of AMR.
The efficacy and mechanism of berberine in improving aging-related cognitive dysfunction: A study based on network pharmacology
Authors: Yao Jiuxiu , Wei Wei , Wen Jiayu , Cao Yu , Li Hao 
Published: January 2023

ObjectiveTo analyze the effects and mechanisms of berberine in the treatment of aging-related cognitive dysfunction based on network pharmacology methods, molecular docking techniques, and animal experiments.MethodsA mouse model of cognitive dysfunction was constructed by subcutaneous injection of D-galactose (D-gal) for 10 weeks, and the neuroprotective effects of berberine on aging-related cognitive dysfunction mice were evaluated by the Morris water maze (MWM) and immunofluorescence staining. The targets of berberine were obtained by SwissTargetPrediction, GeneCards, and PharmMapper. Putative targets of cognitive dysfunction were obtained by GeneCards, TTD, and DrugBank database. The STRING database and Cytoscape software were applied for protein-protein interaction (PPI) analysis and further screening of core targets. The DAVID database was used for Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) enrichment analysis to clarify the biological processes and pathways involved in the intersection targets, and AutoDockTools was adopted for molecular docking verification of core targets. Finally, the core genes were validated using real-time quantitative PCR.ResultsThe MWM results showed that treatment with berberine significantly improved spatial learning and memory in mice with cognitive decline induced by D-gal. Immunofluorescence staining indicated that berberine modified the levels of aging-related markers in the brain. A total of 386 berberine putative targets associated with cognitive dysfunction were identified based on the public database. The core targets of berberine for improving cognitive function, include Mapk1, Src, Ctnnb1, Akt1, Pik3ca, Tp53, Jun, and Hsp90aa1. GO enrichment and KEGG pathway enrichment analyses indicated that the mechanism of berberine in the treatment of aging-related cognitive dysfunction is attributed to pathways such as PI3K-AKT and MAPK pathways. In vivo experiments further confirmed that Akt1, Ctnnb1, Tp53, and Jun were involved in the neuroprotective actions of berberine.ConclusionThis study reveals the multi-target and multi-pathway effects of berberine on regulating aging-related cognitive dysfunction, which provides preclinical evidence and may promote new drug development in mitigating cognitive dysfunction.
High risk-myelodysplastic syndrome following CAR T-cell therapy in a patient with relapsed diffuse large B cell lymphoma: A case report and literature review
Authors: Accorsi Buttini Eugenia , Farina Mirko , Lorenzi Luisa , Polverelli Nicola , Radici Vera , Morello Enrico , Colnaghi Federica , Almici Camillo , Ferrari Emilio , Bianchetti Andrea , Leoni Alessandro , Re Federica , Bosio Katia , Bernardi Simona , Malagola Michele , Re Alessandro , Russo Domenico 
Published: January 2023

BackgroundChimeric antigen receptor (CAR) T-cell therapy represents the most advanced immunotherapy against relapsed/refractory B cell malignancies. While cytokine release syndrome and immune effector cell-associated neurotoxicity syndrome are distinctive, known CAR T-cell acute adverse events, hematological toxicity has been increasingly reported. Cytopenia following CAR T-cell treatment is attributed in most cases to lymphodepletion regimens, bridging chemotherapy, or radiotherapy. However, when cytopenia becomes prolonged, the development of myelodysplastic syndrome (MDS) should be considered.Case presentationWe report a case of high risk (HR)-MDS following CAR T-cell therapy in a patient with relapsed diffuse large B cell lymphoma. Eight months after CAR T-cell infusion, the blood count showed progressive, worsening cytopenia and the bone marrow biopsy revealed multilineage dysplasia without excess of blasts associated with chromosome 7 deletion and RUNX1 mutation. Next generation sequencing analysis, retrospectively performed on stored samples, showed a germ line CSF3R mutation, CEBPA clonal hematopoiesis, but no RUNX1 lesion.ConclusionWe describe a case of HR-MDS, with deletion of chromosome 7 and acquisition of RUNX1 mutation, developing after CAR T-cell therapy in a patient with clonal hematopoiesis (CH). Previous chemotherapy favored MDS onset; however, we could not exclude the fact that the impairment of immunosurveillance related to either lymphodepletion or CAR T-cell infusion may play a role in MDS development. Thus, we designed a multicenter prospective study (ClonHema-CAR-T-Study) to investigate if cytopenia after CAR T-cell treatment may be due to underling CH as well as the presence of secondary myeloid malignancies.
Age independent survival benefit for patients with small hepatocellular carcinoma undergoing percutaneous cryoablation: A propensity scores matching study
Authors: Zhang Huixin , Xu Muoyan , Shao Jiashen , Kong Huifang , Gao Xudong , Zhang Wei , Chang Xiujuan , Yang Bin , Chen Yan , Dong Zheng , Huang Jiagan , Zeng Zhen , Yang Yongping 
Published: January 2023

BackgroundHepatocellular carcinoma (HCC) is the major cause of malignancy-related deaths worldwide, and its incidence is likely to increase in the future as life expectancy increases. Therefore, the management of elderly patients with HCC has become a global issue. Aim of this study was to assess whether elderly patients with small HCC could obtain survival benefit from cryoablation (CRYO) in a real-world.Materials and methodsFrom July 2007 to June 2013, 185 patients with small HCC who underwent curative-intent percutaneous CRYO. All patients were divided into three groups according to age distribution. Overall survival (OS) and tumor-free survival (TFS) were compared between among of groups before and after the 1:1 propensity score matching, respectively. Univariate and multivariate Cox analyses were performed to determine the potential relationships between variables and prognostic outcomes.ResultsOne hundred and eighty-five patients (144 men, 41 women) received CRYO for small HCC, including 59 patients with age &lt;50 years, 105 patients with age between 50 and 65 years, and 21 patients with age &gt;65 years. The three age groups showed significant differences in the terms of underlying chronic liver disease and the number of patients with minor postoperative complications. After propensity score matching, the younger and elderly groups showed significant differences in mean OS (P=0.008) and tumor progression (P=0.050). However, no significant differences were shown in mean progression-free survival (PFS) (P=0.303). The Cox multivariate analysis showed that the Child-Pugh grade (HR=3.1, P&lt;0.001), albumin (HR=0.85, P=0.004) and total of bilirubin (HR=1, P=0.024) were the independent prognostic factor for mean OS.ConclusionOur propensity-score-matched study suggested that elderly patients with small HCC can achieve acceptable prognostic outcomes with PFS similar to those of younger patients with small HCC after treatment with CRYO, while Child-Pugh grade, bilirubin and serum albumin levels were associated with the prognosis of small HCCs.
A low phase noise high power Er-fiber frequency comb synchronized to the hydrogen maser clock by harmonic phase locking
Authors: Li Mingkun , Yang Xiguang , Rao Bingjie , Yan Lulu , Chen Xin , Yuan Ru , Zhang Pan , Zhang Yanyan , Liu Tao 
Published: January 2023

We demonstrate a low phase noise all polarization-maintaining (PM) Er-fiber optical frequency comb (OFC) with low phase noise, which is synchronized to the hydrogen maser clock (HMC) using the 18th harmonic of the repetition rate for tight phase locking. The instability of the locked carrier envelope offset frequency is 1.24 × 10−18@1 s, the phase noise of the OFC is −96 dBc/Hz at 1 Hz offset, and the corresponding RMS timing jitter of the repetition rate is 62 fs (1 Hz–1 MHz). The residual frequency instability of the repetition rate (200 MHz) is 1.46 × 10−14@1 s, and the residual phase noise of the OFC normalized to 10 MHz is −138 dBc/Hz at 1 Hz offset and declines to about −160 dBc/Hz at the far-end, much lower than that of the HMC (−122 dBc/Hz at 1 Hz and −156 dBc/Hz at the far-end).
Adherence and effectiveness of an emotion-based psychodynamic online self-help during and after inpatient and day-care psychotherapy: Results of a naturalistic study
Authors: Becker Jan , Kreis Adina , Schorch Theresa , Mayer Anna , Tsiouris Angeliki , Beutel Manfred E. , Zwerenz Rüdiger 
Published: January 2023

BackgroundInternet-based mental health interventions are considered effective in providing low-threshold support for people with mental health disorders. However, there is a lack of research investigating the transferability of such online programs into routine care settings. Low treatment adherence and problems with technical implementation often limit a successful transfer into clinical routines. This naturalistic study aims to identify influencing factors on program adherence in patients who participated in an online intervention during inpatient or day-clinic psychotherapeutic treatment.Methods and findingsIn a naturalistic study, we investigated the transferability of the transdiagnostic psychodynamic online self-help program KEN-Online, which includes eight consecutive units. Between May 2017 and October 2018, patients who received inpatient or day-clinic psychotherapeutic treatment at the Department of Psychosomatic Medicine and Psychotherapy in the University Medical Center Mainz have been offered to use KEN-Online. Of the n = 749 patients who were admitted to the clinic, n = 239 patients (32%) registered for participation in KEN-Online. While 46.9% of the participants did not complete any unit (inactive participants), 53.1% completed at least the first unit (active participants). Age, number of diagnoses, and symptom severity were associated with (in)active participation. Adherence decreased over time resulting in only 17 participants (7.6%) who completed all units. None of the sociodemographic and medical characteristics proved to be significant predictors of adherence. Analyses of effectiveness showed a significant reduction of anxiety and depression in active participants in the course of participation, with higher improvements in participants that completed more than half of the units.ConclusionAdherence to the online self-help program KEN-Online was lower in the naturalistic setting than in a previous clinical trial, but was still associated with greater program effectiveness. Adherence-promoting measures are crucial to increase the effectiveness of such interventions in clinical settings.
Agile Delphi methodology: A case study on how technology impacts burnout syndrome in the post-pandemic era
Authors: Medina-Dominguez Fuensanta , Sanchez-Segura Maria-Isabel , de Amescua-Seco Antonio , Dugarte-Peña Germán-Lenin , Villalba Arranz Santiago 
Published: January 2023

IntroductionIn the post-pandemic era, many habits in different areas of our lives have changed. The exponential growth in the use of technology to perform work activities is one of them. At the same time, there has been a marked increase in burnout syndrome. Is this a coincidence? Could they be two interconnected developments? What if they were? Can we use technology to mitigate this syndrome? This article presents the agile Delphi methodology (MAD), an evolved version of the Delphi method, adapted to the needs of modern-day society.MethodsTo drive Occupational Health and Safety (OHS) experts to reach a consensus on what technological and non-technological factors could be causing the burnout syndrome experienced by workers in the post-pandemic era, MAD has been used in a specific case study. This study formally presents MAD and describes the stages enacted to run Delphi experiments agilely.ResultsMAD is more efficient than the traditional Delphi methodology, reducing the time taken to reach a consensus and increasing the quality of the resulting products.DiscussionOHS experts identified factors that affect and cause an increase in burnout syndrome as well as mechanisms to mitigate their effects. The next step is to evaluate whether, as the experts predict, burnout syndrome decreases with the mechanisms identified in this case study.