bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of RCN1 inhibits esophageal squamous cell carcinoma progression and M2 macrophage polarization
Authors: Guo Haiyang , Su Jinghao , Hu Guangbing , Liu Bingyang , Li Jie , Sun Jinhong , Wang Xiaobo , Liu Han , Xiong Shiyu , Tang Yong , Yin Yaolin , Wang Xianfei 
Published: August 2023

Abstract

Background: Reticulocalbin 1 (RCN1) is a calcium-binding protein involved in the regulation of calcium homeostasis in the endoplasmic reticulum. The aim of this study was to explore the clinical value and biological role of RCN1 in esophageal squamous cell carcinoma (ESCC). In addition, we also investigated the effect of RCN1 on the polarization of tumor-associated macrophages (TAMs).
Methods: The GSE53625 dataset from the Gene Expression Omnibus database was used to analyze the expression of RCN1 mRNA and the relationship with clinical value and immune cell infiltration. Immunohistochemistry was used to validate the expression of RCN1 and its correlation with clinicopathological characteristics. Subsequently, transwell and cell scratch assays were conducted to evaluate the migration and invasion abilities of ESCC cells. The expression levels of epithelial–mesenchymal transition (EMT)-related proteins were detected using Western blotting, while flow cytometry and Western blotting were employed to detect cell apoptosis. Additionally, qRT-PCR and Western blotting were utilized to evaluate the role of RCN1 in macrophage polarization.
Results: RCN1is significantly upregulated in ESCC tissues and is closely associated with lymphatic metastasis and a poor prognosis; it is an independent prognostic factor for ESCC patients. Knockdown of RCN1 significantly inhibits migration, invasion, and EMT of ESCC cells, and promotes cell apoptosis. In addition, RCN1 downregulation inhibited the polarization of M2 macrophages.
Conclusion: RCN1is upregulated in ESCC patients and is negatively correlated with patient prognosis. Knocking down RCN1 can inhibit the progression of ESCC cells and polarization of M2 macrophages. RCN1 could serve as a potential diagnostic and prognostic indicator for ESCC, and targeting RCN1 is a very promising therapeutic target.
Single-Cell Transcriptomics Analysis Reveals the Promotion of Mitochondrial Hyperactive Microglial Subpopulations by CSF1R following Stroke
Authors: Zou Qing , Wu Hao , Zhou Jianxin , Tian Guangzhou , Fu Wenjun , Zhao Xuyu , Ward Natalie , Wang Ruyi , Zhao Robert Chunhua , Wang Jiao 
Published: August 2023

Abstract
Stroke is a medical emergency that occurs due to interruption or reduction of the blood supply to the brain, which results in brain tissue damage. Despite mitochondria are damaged to a certain extent after cells are subjected to oxygen/sugar deprivation following stroke, clinical data have not demonstrated mitochondrial damage aggravates the progression of stroke. In the present study, single-cell RNA sequencing (scRNA-seq) data of 29,388 cells from the hippocampus of normal mice and those with ischemic or hemorrhagic stroke were obtained and further analyzed. Our data reveal extensive heterogeneity among immune cells, with microglia exhibiting distinct transcriptomic signatures. In-depth bioinformatics analysis identified the mitochondrial leucine-tRNA synthetase 2 (Lars2) as being aberrantly expressed in multiple cell types. Additionally, stroke-specific microglial subpopulations exhibited similar expression patterns to those of disease-associated microglia (DAM). The Csf1-Csf1r pathway was highly activated in microglia during the autocrine process following stroke, and Csf1r is highly expressed in microglia and macrophages in the central nervous system, suggesting Csf1r promotes the subpopulation of microglia with highly active mitochondria following stroke. Our results implied that blockade of the Csf1r signaling pathway may have therapeutic significance in neuroinflammatory diseases such as stroke. These findings provide new insights into cell subtype diversity and molecular characteristics following brain injury from the perspective of microglia and mitochondria.
SARS-CoV-2 Spike Protein Accelerates Systemic Sclerosis by Increasing Inflammatory Cytokines, Th17 Cells, and Fibrosis
Authors: Jeong Ha Yeon , Park Jin-Sil , Woo Jin Seok , Lee Kun Hee , Choi Jeong Won , Kang Hye Yeon , Na Hyun Sik , Lee Yeon Su , Um In Gyu , Park Sung-Hwan , Cho Mi-La 
Published: August 2023

Abstract
Background
Coronavirus disease 2019 (COVID-19) induces a dysfunctional immune response, inflammation, autoantibody production, and coagulopathy, which are symptoms that bear resemblance to those of autoimmune diseases, including systemic sclerosis (SSc).
Methods
While there is a single case report suggesting an association between COVID-19 and SSc, the effects of COVID-19 on SSc are not yet fully understood. Human embryonic kidney 293 (HEK293) cells were transfected with the SARS-CoV-2 spike protein gene, both in the absence and presence of TGF-β. The expression levels of fibrosis-related proteins were measured via Western blotting. A bleomycin (BLM)-induced SSc mouse model was employed, wherein mice were injected with the gene encoding the SARS-CoV-2 spike protein and the ACE2 receptor. The levels of fibrosis, autoantibodies, thrombotic factors, and inflammatory cytokines in tissues and serum were analyzed.
Results
In vitro, the expression levels of fibrosis marker proteins were elevated in the spike protein group compared to the control group. In vivo, the skin thickness of SSc mice increased following exposure to the SARS-CoV-2 spike protein. Furthermore, the levels of autoantibodies and thrombotic factors, such as anti-phospholipid antibodies (APLA), were significantly increased in the presence of the protein. Flow cytometry analysis revealed increased expression of the proinflammatory cytokine IL-17 in the skin, lungs, spleen, and blood. Moreover, tissue fibrosis and levels of inflammatory cytokines in skin and lung tissues were markedly escalated in SSc mice subjected to the protein.
Conclusion
COVID-19 may accelerate the development and progression of SSc by intensifying fibrosis through the upregulation of inflammation, autoantibody production, and thrombosis.
Filtered Low-power Multi-high-gain Observer Design for a Class of Nonlinear Systems
Authors: Mousavi Moein , Guay Martin 
Published: August 2023

Abstract
In this paper, a high-gain observer (HGO) approach is used to design a nonlinear observer for nonlinear systems in canonical observer form. The design uses multiple low-power (LP) observers with low-pass filters that provide a state estimation approach as a weighted sum of the individual observer state variables. As for the typical HGO design, it is shown that the estimation error dynamics of the proposed observer are input-to-state stable (ISS) with regard to measurement noise with a tunable convergence rate. It is also demonstrated that the multiple LP observer yields a smaller bound on the estimation error than the HGO and LP. Finally, the steady-state behaviour of the observer is characterized. For the linear case, the observer improves the sensitivity of the state estimates to measurement noise. The effectiveness of the suggested approach is demonstrated for the identification of a robot arm for which the observer is used as a numerical differentiator.
Construction and metric properties of a new instrument to measure adaptive behaviour in people with intellectual disabilities. Peruvian context.
Authors: Carbajal Alicia Boluarte , Ventura Gina Chavez , Vargas Jorge Cueva 
Published: August 2023

Abstract
Background Adaptive behaviour is an important characteristic of people with intellectual disabilities, and it has been associated with the individual’s requirements to performance adequately in the social context. Indeed, measuring adaptive behaviour indicates what an individual does independently, without help, support, reminders, or prompts. Peru has a particular society and there is not an accessible scale to evaluate the adaptive behaviour in people with intellectual disabilities. We proposed to create and obtain evidence of validity and reliability of a new Adaptive Behaviour Test for people over 15 years old with intellectual disabilities with interest in being part of the economically active population.Methods We used a qualitative approach to construct the test, the Aiken V coefficient to assess content validity, and Krippendorff's alpha for inter-observer reliability. We performed an exploratory and confirmatory factor analysis to assess the internal structure and consistency to check the homogeneity between the proposed items. We performed convergent validity to analyze the relationship of the test with other variables.Results We obtained a 25-item test with three dimensions: a) activities of daily living; b) instrumental skills and c) communication. Aiken's V coefficient, Krippendorff's alpha, factor analysis and internal consistency allowed us to identify the most important items and confirm the factors. convergent validity with quality of life was demonstrated.Conclusion The developed scale is a useful screening test for the measurement of adaptive behavior and the monitoring of social and labor inclusion programs, the results show that the items and factors contribute to the measurement of the construct.
Clinical effect of defocus incorporated multiple segments (DIMS) spectacles combined with low-concentration atropine in fast-progressing myopic children
Authors: Xu Jin , Hao Qian 
Published: August 2023

Abstract
Purpose
To compare the clinical efficacy of DIMS spectacles combined with 0.01% or 0.05% atropine in fast-progressing myopic children with DIMS spectacles alone.
Methods
We conducted a retrospective study of 93 patients aged 8–12 years who attended the optometry clinic at Shenzhen Aier Eye Hospital between January 2021 and February 2023. The subjects were selected based on their use of DIMS spectacles alone in the first year. In the second year, the patients were divided into three groups: continued to use DIMS spectacles alone (S Group), combined DIMS spectaeles with 0.01% atropine (SA1 Group), and combined DIMS spectacles with 0.05% atropine (SA5 Group).
Results
After a 2-year review, it was found that there was no significant difference in the growth of myopia and axial elongation among the three groups in the first year (P = 0.91; P = 0.23). In the second year, there were no significant differences in the growth of myopia and axial elongation between the S group and the SA1 group (P = 0.50; P = 0.35). Similarly, no significant differences were found between the S group and the SA5 group (P = 0.00; P = 0.00). However, significant differences were observed between the SA1 group and the SA5 group (P = 0.00; P = 0.00).
Conclusions
For children with rapid myopia growth using DIMS spectacles alone, the effects of combining 0.01% atropine were not obvious. However, when combined with 0.05% atropine, the effects were noticeable.
Genome-wide Identification, characterization and evolutionary analysis of C2H2-type zinc finger proteins (ZFPs) in Hordeum vulgare under salt stress
Authors: Maghraby Amaal , AlZalaty Mohamed 
Published: August 2023

Abstract
Genome-wide identification of C2H2-type zinc finger proteins were identified 77 C2H2-type zinc finger proteins in H. vulgare. Due to the importance of the understanding stress response mechanisms, the current study were carried out to study the salinity stress response genes, qRT-PCR results were could identify the zinc finger protein 36 (HvZFP36) as salinity-responsive and also keeping in view that the HvZFP36 protein were down regulated with fold change 0.16 under salt stress. Domain analysis confirmed the presence of the Zinc finger C2H2-type domain on 77 Znf_C2H2 protein sequences. Motifs and introns/exons structure analysis indicates, that genes with closer phylogenetic relationships exhibited more similar genetic structures. The most HvZnf_C2H2 proteins were located in the nucleus and 20 putative nuclear localization signal (NLS) were predicted against 14 HvZnf_C2H2 proteins. Transmembrane helical were predicted the in HvZnf_C2H2_43 and HvZnf_C2H2_77 proteins and HvZnf_C2H2_9 protein were have been found to be more phosphorylated with serine, threonine and tyrosine. 3-D prediction of C2H2-type zinc finger proteins models have a C-score range from − 1.27 to − 3.39, which suggesting the structures proteins are constructed with high accuracy. 34 microRNAs were predicted against 30 HvZnf_C2H2 genes. HvZnf_C2H2 genes were physically located on the 7 chromosomes in H. vulgare genome. Ka/Ks ratio were indicated that the HvZnf_C2H2 genes were primarily influenced by purifying selection and we were suggested HvZnf_C2H2_7/HvZnf_C2H2_26 paralogous gene pairs were have a basic role of increase number of HvZnf_C2H2 genes family in H. vulgare. Phylogenetic analysis were classified HvZnf_C2H2 proteins into three clades. Synteny analysis resulting, 10 C2H2-type zinc finger proteins from clade I were have collinearity orthologous relationship in Zea mays, Solanum lycopersicum and Arabidopsis thaliana. Clade II were not have collinearity orthologous relationship in A. thaliana. HvZnf_C2H2_20 in clade III were have collinearity orthologous relationship in A. thaliana. The gene ontology enrichment analysis were confirmed the functional role of HvZnf_C2H2 as a stress responsive.
Predicting future soil carbon dynamics under global change scenarios from a temperate deciduous forest
Authors: Qu Ruosong , Chen Shiyi , Wang Kefeng , Liu QiUyu , Yang Bin , Yue Ming , Peng Changhui 
Published: August 2023

Abstract

Purpose
Soil carbon cycling processes in terrestrial ecosystems are significantly influenced by global changes, and soil microorganisms play a crucial role in soil organic carbon (SOC) and its feedbacks to climate change.
Methods
To investigate the potential future changes in soil carbon dynamics under different scenarios in the Ziwuling Forest region, we collected soil data through field observation experiments at a long-term fixed observation sample site. By utilizing a microbial ecological model (MEND), we predicted the future dynamics of soil carbon under different scenarios in the area.
Results
We found that under the RCP2.6 scenario, SOC showed a rapid increase. By 2100, the SOC values in topsoil are projected to be higher than whole soil SOC values for the three RCP scenarios. SOC under the RCP2.6 scenario will be significantly higher than those under the RCP4.5 scenario and RCP8.5 scenario. Furthermore, the positive correlation between total litter carbon (LC) and SOC under the RCP2.6 scenario highlights the potential role of total litter carbon in driving SOC dynamics. We also found that the low greenhouse gas (GHG) emission scenario favors the accumulation of SOC in the study area, while the high GHG emission scenario leads to greater soil carbon loss.
Conclusions
Our results underscore the importance of considering the impact of climate change(especially global warming)on soil ecosystems in the future. Protecting the soil ecosystem of the Loess Plateau is critical for maintaining soil carbon sinks, preventing soil erosion, and improving and regulating the surrounding environmental climate.
Bevacizumab increases the sensitivity of olaparib to homologous recombination-proficient ovarian cancer by suppressing CRY1 via PI3K/AKT pathway
Authors: Iida Yasushi , Yanaihara Nozomu , Yoshino Yuki , Saito Misato , Saito Ryosuke , Tabata Junya , Kawabata Ayako , Takenaka Masataka , Chiba Natsuko , Okamoto Aikou 
Published: August 2023

Abstract
PARP inhibitors have changed the management of advanced high-grade epithelial ovarian cancer (EOC), especially homologous recombinant (HR)-deficient advanced high-grade EOC. However, the effect of PARP inhibitors on HR-proficient EOC is limited. Thus, new therapeutic strategy for HR-proficient EOC is desired. In recent clinical study, the combination of PARP inhibitors with anti-angiogenic agents improved therapeutic efficacy, even in HR-proficient cases. These data suggested that anti-angiogenic agents might potentiate EOC cells to PARP inhibitors. Here, we demonstrated that anti-angiogenic agents, bevacizumab and cediranib, increased the sensitivity of olaparib in HR-proficient EOC cells by suppressing HR activity. RNA sequencing showed that bevacizumab decreased the expression of CRY1 under DNA damage stress. We found that the anti-angiogenic agents suppressed the increase of CRY1 expression by inhibiting VEGF/CFGFR/PI3K pathway. The suppression of CRY1 expression resulted in decrease of HR activity. In addition, CRY1 inhibition also sensitized EOC cells to olaparib. These data suggested that anti-angiogenic agents and CRY1 inhibitors will be the promising candidate in the combination therapy with PARP inhibitors in HR-proficient EOC.
Artificial intelligence-driven shimming for parallel high field nuclear magnetic resonance
Authors: Becker Moritz , Cheng Yen-Tse , Voigt Achim , Chenakkara Ajmal , He Mengjia , Lehmkuhl Soeren , Jouda Mazin , Korvink Jan 
Published: August 2023

Abstract
Rapid drug development requires a high throughput screening technology. NMR could benefit from parallel detection but is
hampered by technical obstacles. Detection sites must be magnetically shimmed to ppb uniformity, which for parallel detection is precluded by commercial shimming technology. Here we show that, by centering a separate shim system over each detector and employing deep learning to cope with overlapping non-orthogonal shimming fields, parallel detectors can be rapidly calibrated. Our implementation also reports the smallest NMR stripline detectors to date, based on an origami technique,
facilitating further upscaling in the number of detection sites within the magnet bore.
Codified Barriers to Mental Health Care, an Example from New York State
Authors: Richardson Sandra 
Published: August 2023

Abstract
This analysis illustrates the impact of codified barriers to mental health care such as the New York Secure Ammunition and Firearms Enforcement Act (NY SAFE Act). New York State Division of Criminal Justice Services (DCJS) data, United States Census data, and existing literature were utilized to quantify the barrier to mental health care created by NY SAFE Act reporting and effectiveness in identifying the target population. For each DCJS database search related to such reports, about 12 adults who received mental health services in the past year are less likely to seek mental health care; and about 16 adult gun owners are less likely to seek mental health care because of this law. This reporting captures approximately 2.7% of individuals who are suicidal or homicidal. These results indicate that NY’s SAFE Act reporting has created a large barrier to mental health care and is ineffective in capturing its target population.
Association between chronic neck pain and sleep duration in US adults: A cross-sectional study
Authors: Yang Yanlin , Tang Shaoqun 
Published: August 2023

Abstract
The relationship between sleep duration and chronic neck pain is poorly understood. Therefore, we explore this association by compiling data from the National Health and Nutrition Examination Survey (NHANES) ranging from 2009 to 2010, including 3036 adults (age ≥ 20 years). We selected sex, gender, education level, race, marital status, alcohol status, smoking status, hypertension, hyperlipidemia, PIR(poverty-income ratio), and BMI༈Body mass index༉ as covariates. WTMEC2YR as weights for multivariate logistic regression models analysis. In the total population, the second, third, and fourth quartiles had an OR of 0.56 (95%CI: 0.38, 0.84); 0.40 (95%CI: 0.24,0.66); 0.77(95%CI: 0.32,1.83) compared with the lowest reference group of sleep duration for chronic neck pain (Ptrend=0.03). When sleep duration is less than 7.45 hours, sleep duration has a negative relationship with the incidence of chronic neck pain (P < 0.05). And the optimal sleep hours show the difference among the gender; the optimal sleep time in the female group is 6.27 hours (P < 0.001), and in the male group is 7.75 hours (P < 0.001).
Assessing wind energy potential and economics for three offshore locations on the Persian Gulf in Iran
Authors: Yousefpour Khalegh 
Published: August 2023

Abstract
Wind energy has been increasingly used in recent years, and is potentially competitive with conventional fuels in many locations. The spatial dependence is significant and related to the speed and energy of the winds. In this paper, energy and economic feasibility assessments of offshore winds are carried out, by considering the use of wind turbines in Abu Musa, Qeshm and Kish islands, located in the south of Iran. The analyses utilize the Weibull density function and recorded data for a four-year period (2019–2022), taken at three-hour intervals and at heights of 10 and 50m. The hourly, daily, monthly and annual variations of wind speed are analyzed, and the economic feasibility is determined of installing wind turbine sat the sites. Annual values for the Weibull shape parameters for Abu Musa, Qeshm and Kish islands are found to be in the range of 2.39–2.10, 2.28–2.54 and 2.04–2.10, respectively. The maximum wind power densities for Abu Musa, Qeshm and Kish islands occur in January, April and March, respectively. The results show that the wind potential and the generated electricity with two wind turbines models are suitable for the studied sites, and that the cost of generating electricity is lower than the price offered by the Iranian government to private power plants.
Combined Associations of Genetic and Environmental Risk Factors in Age of Parkinson’s Disease Onset
Authors: Li Jinchen , Huang Yuanfeng , Chen Qian , Wang Zheng , Wang Yijing , Lian Aojie , Zhou Qiao , Zhao Guihu , Xia Kun , Tang Beisha , Li Bin 
Published: August 2023

Abstract
Substantial evidence has shown that the age at onset (AAO) of Parkinson’s disease (PD) is a major determinant of clinical heterogeneity. However, the mechanisms underlying heterogeneity in the AAO remain largely unknown. To investigate the independent association of genetic and environmental risk factors with the AAO of PD and their combined effects at a genome-wide level. A total of 3,156 patients with PD from the UK Biobank were included in this study. We evaluated the environmental risk factors associated with AAO using the Mann–Whitney U test and a generalized linear model. We further investigated the effects of genetic factors using linear regression analysis and their interactions with environmental risk factors using genome-wide by environment interaction studies. In addition to identifying previously reported environmental risk factors (smoking, non-steroidal anti-inflammatory drug intake, and family history of PD) associated with AAO, we found novel significant associations of physical activity (p < 0.0001) and excessive daytime sleepiness (p < 0.0001) with the AAO of PD. Individuals with a higher genetic risk had younger AAO (p = 3.91e-05). Additionally, we observed a nominally significant interaction between the polygenic risk scores and smoking for AAO (p = 0.0316). Specifically, several gene including ANGPT1 (p = 7.17e-07) and PLEKHA6 (p = 4.87e-06), suggestively significantly interacted with smoking to influence the AAO. Our data suggests that both genetic and environmental risk factors are associated with the AAO of PD and that there is a potential interaction between the two.