bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application value of multi-disciplinary collaborative diagnosis and treatment combined with CBL teaching model in gynecological oncology practice teaching
Authors: Wen Fengrong , Mo Julan , Li Renxiang 
Published: January 2025

BackgroundTo explore the application value of multi-disciplinary collaborative diagnosis (MDT) and treatment combined with the case-based learning (CBL) teaching method based on real clinical cases in gynecological malignant tumor practice teaching.MethodsA total of 120 clinical students who were interning in the Department of Gynecology in our hospital from January 2022 to June 2023 were selected and divided into a research group (n = 60) and a control group (n = 60) according to the random number table method. The research group adopted a MDT combined with the CBL teaching model, while the control group followed a traditional teaching model. After the two-month internship, the teaching faculty completed a self-evaluation form, and the students jointly evaluated the teaching effect through an exit assessment, which included basic theory, clinical skills, and case assessment. Additionally, a questionnaire survey was conducted to evaluate the student’s recognition of the teaching model and collect their opinions and suggestions.ResultsThe research group showed significantly higher scores in basic theoretical knowledge, clinical skills, and case analysis assessments compared to the control group (p &lt; 0.05). The questionnaire survey results indicated that the research group outperformed the control group in knowledge acquisition, learning initiative, learning interest, clinical analysis ability, clinical diagnosis, treatment thinking, teamwork ability, literature retrieval, and reading ability, and clinical language expression ability (p &lt; 0.05). Students’ feedback suggested increasing doctor-patient communication time and improving the doctor-patient communication skills.ConclusionMDT combined with the CBL teaching model based on real clinical cases can effectively foster autonomous learning, enhance the application of basic theoretical knowledge, and improves the quality of clinical teaching in gynecology. This method is worthy of promotion in clinical teaching.
Analysis of ASAS health index and its influencing factors in ankylosing spondylitis: a prospective study based on the population of Chaoshan region
Authors: Li Aikang , Liang Rongji , Wu Liangbin , Cai Minghua , Chen Jiayou , Gong Yao , Zeng Shaoyin 
Published: January 2025

ObjectiveThis study aimed to evaluate the health-related quality of life (HRQoL) in ankylosing spondylitis (AS) patients in the Chaoshan region and identify factors influencing the ASAS Health Index (ASAS-HI) to enhance comprehensive AS treatment strategies.MethodsA survey of ASAS-HI was conducted on 82 AS patients from the rheumatology outpatient department of the First Affiliated Hospital of Shantou University Medical College. The Bath Ankylosing Spondylitis Global Score (BAS-G) assessed overall health status, the Ankylosing Spondylitis Quality of Life Questionnaire (AS-QOL) evaluated quality of life, the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) measured disease activity, and the Bath Ankylosing Spondylitis Functional Index (BASFI) assessed functional difficulties. Inflammatory markers and patient data were collected, and univariate/multivariate logistic regression analyses were used to explore influencing factors of ASAS-HI.ResultsThe mean ASAS-HI score was 3.52 ± 3.12. ASAS-HI was positively correlated with BASDAI (r = 0.478, p &lt; 0.001), ASDAS-CRP (r = 0.406, p &lt; 0.001), BASFI (r = 0.338, p &lt; 0.002), and BAS-G (r = 0.335, p &lt; 0.002). Patients with ASDAS-ESR ≥ 2.1, ASDAS-CRP ≥ 2.1, and spinal tenderness had significantly higher ASAS-HI scores than others (p &lt; 0.001). Spinal tenderness and radiographic grading were identified as key influencing factors.ConclusionASAS-HI is significantly impacted by disease activity and functional limitations. Early assessment of ASAS-HI is crucial for optimizing disease management in AS patients.
Analysis of the relevant factors for corneal graft rejection in the southern Liaoning region from 2019 to 2023
Authors: Yan Chunxiao , Zhang Zhijian , Jin Lin , Liu Mengxin , Wang Tianyi , Yang Jinghao , Zhang Lijun 
Published: January 2025

BackgroundThe study aimed to review the etiology of corneal blindness and investigate the relative risk of corneal graft rejection (CGR) in the southern Liaoning region.MethodsThe clinical records of 359 patients (394 eyes) who underwent corneal transplantation at the Department of Keratoconus of the Third People’s Hospital of Dalian from January 2019 to December 2023 were retrospectively analyzed. The data included patients’ age, gender, occupation, diagnosis, surgical procedure, postoperative immune rejection, and neovascularization. The data were collected and descriptively analyzed to characterize the etiology of corneal blindness and to analyze the risk factors for postoperative immune rejection after corneal transplantation using logistic regression.ResultsThe mean age of the patients who underwent corneal transplantation was 55.90 ± 0.80 years, and there were more male patients than female patients with corneal blindness. Infectious keratitis (41.1%) was reported as the leading cause of corneal blindness, and penetrating corneal transplantation was the main surgical procedure for the recovery of sight. Preoperative corneal vascularization and penetrating corneal graft rejection were identified as risk factors for immune rejection of corneal grafts. The preoperative corneal vascularization was performed (p = 0.044, OR = 2.607). Penetrating keratoplasty (PKP) was performed (p = 0.024, OR = 1.953), and deep anterior lamellar keratoplasty was also performed (p = 0.801, OR = 1.088). Viral infections (p &lt; 0.001, OR = 16.871) were the major risk factor for preoperative corneal neovascularization (CNV) compared to other etiologies, such as fungal infections (p &lt; 0.001, OR = 0.018), mechanical ocular trauma (p &lt; 0.001, OR = 0.034), immune keratitis (p = 0.023, OR = 0.152), and endothelial dysfunction (p &lt; 0.001, OR = 0.054).ConclusionInfectious keratitis was identified as the major cause of corneal blindness in the southern Liaoning region over the past 5 years. Penetrating keratoplasty and preoperative corneal vascularization were the risk factors for corneal graft rejection. In addition, virus-derived keratitis was considered to be the main risk factor for corneal neovascularization, and deep anterior lamellar keratoplasty was not found to have an effect on corneal graft rejection in this study.
Long-term rice-crab coculturing leads to changes in soil microbial communities
Authors: Ma Liang , Yang Tiexin , Dong Liqiang , Pan Zhengyan , Feng Yingying , Li Zhiqiang , Sun Fuyu 
Published: January 2025

PurposeIn order to investigate the effects of a rice-crab coculture mode and its duration on the richness and diversity of the soil microbial community.MethodSoil from long-term rice-crab coculture mode (MY), newly established rice-crab coculture mode (OY) and rice monoculture mode (N) were used to measured soil physicochemical properties, enzyme activity and 16S and ITS soil microbial communities.ResultsThe results revealed that in terms of mode, the MBC, MBN and CAT of OY were significantly greater than those of N by 10.75, 23.47 and 30.71% (p &lt; 0.05), respectively. The richness and diversity of the soil microbial communities changed little, and there was no difference in the main species. In terms of duration, the OM, SC and PPO contents of MY were significantly greater than those of OY by 21.96, 41.89 and 11.52% (p &lt; 0.05), respectively. The soil fungi changed significantly, and the main species were Mortierella and Pseudeurotium in genus level. The soil physicochemical properties and soil enzymes explained 93.38 and 93.66%, respectively, of the variation in the soil microbial community, and OM and DHA were the main factors influencing the change in soil biodiversity.ConclusionOur results suggested that long-term rice-crab coculture mode altered the richness and diversity of the soil microbial community and soil carbon sequestration.
The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937
Authors: Wa Yunchao , Zhao Xia , Zhang Chenchen , Qu Hengxian , Chen Dawei , Chen Xia , Huang Yujun , Gu Ruixia 
Published: January 2025

IntroductionThe free exopolysaccharide (f-EPS) produced by Streptococcus thermophilus is a natural texture modifier and has a variety of prebiotic activities. Our previous studies showed f-EPS production from S. thermophilus 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine, and histidine in the chemically defined medium.MethodsIn this study, we used transcriptomics and qPCR to further explore the specific mechanism of the enhanced effect of 3 amino acids on the f-EPS biosynthesis of S. thermophilus 937.ResultsThe mRNA-seq analysis and targeted pathway analysis indicated that genes associated with histidine/valine/leucine/ isoleucine/phenylalanine/tyrosine/tryptophan synthesis, galactose metabolism, purine metabolism and quorum sensing in S. thermophilus 937 were significantly upregulated under increasing concentrations of histidine, isoleucine and glutamate in chemically defined medium (CDM). qPCR results showed that the significant upregulation of galactose metabolism- and nucleotide sugar synthesis-related genes was attributed to the increase in glutamate concentration, and glutamate could induce the expression of galR. The upregulation of epsA, epsB, and epsD transcript levels was caused by the increase in histidine concentration. The upregulation of transcript levels of genes related to phenylalanine/tyrosine/tryptophan/histidine/ valine/leucine/isoleucine synthesis was caused by the increase in isoleucine concentrations.DiscussionThis indicates that 3 amino acids have different mechanisms for enhancing the biosynthesis of f-EPS in S. thermophilus 937.
Levamisole, as a viral vaccine adjuvant, induces robust host defense through the modulation of innate and adaptive immune responses
Authors: Kim Gang Sik , Kwak Dong Yun , Kim Hyeong Won , Shin Seokwon , Ko Mi-Kyeong , Hwang Seong Yun , Park So Hui , Kim Dong Hyeon , Park Jong-Hyeon , Kim Su-Mi , Lee Min Ja 
Published: January 2025

IntroductionAn effective vaccination policy must be implemented to prevent foot-and-mouth disease (FMD). However, the currently used vaccines for FMD have several limitations, including induction of humoral rather than cellular immune responses.MethodsTo overcome these shortcomings, we assessed the efficacy of levamisole, a small-molecule immunomodulator, as an adjuvant for the FMD vaccine. We conducted in vitro studies using murine peritoneal exudate cells (PECs) and porcine peripheral blood mononuclear cells (PBMCs) and in vivo studies using mice (experimental animals) and pigs (target animals). We evaluated levamisole-mediated modulation of the innate and adaptive immune responses; early, mid-term, and long-term immune-inducing effects; modes of action; and host defense against viral infection.ResultsLevamisole treatment promoted IFNγ secretion in murine PECs and porcine PBMCs. Additionally, it induced robust and long-lasting immune responses by eliciting high antibody titers and high virus-neutralizing antibody titers. By activating downstream signaling pathways of various pattern-recognition receptors, levamisole stimulated the expression of multiple cytokines and costimulatory molecules. Owing to these immunostimulatory effects, levamisole elicited host defense against viral infections in pigs. Our findings demonstrate the potential of levamisole as an immunostimulatory agent.DiscussionThe results also indicate that levamisole, as an adjuvant for animal vaccines, can elicit robust innate and adaptive immune responses, thereby enhancing host defense against viral infections. This study provides a promising approach for the development of improved FMD vaccine strategies in the future.
Insights into microbial compositions of the respiratory tract of neonatal dairy calves in a longitudinal probiotic trial through 16S rRNA sequencing
Authors: Tan Jia W. , Eicher Susan D. , Kritchevsky Janice E. , Bryan Keith A. , Dickey Aaron , Chitko-McKown Carol G. , McDaneld Tara G. 
Published: January 2025

IntroductionProbiotics are a promising intervention for modulating the microbiome and the immune system, promoting health benefits in cattle. While studies have characterized the calf lung bacterial profile with and without oral probiotics, simultaneous probiotic effects on the bacterial populations of multiple sites along the respiratory tract have not been characterized.MethodsThis study utilized the same pre-weaning diary calf group from our previous studies to characterize the bacterial populations present in the nostril and tonsil across control and treatment groups and nine sampling time points. DNA was exacted from the nostril and tonsil swabs and lung lavage fluids, and 16S ribosomal RNA gene hypervariable regions 1-3 were subsequently sequenced.ResultsTemporal variation in alpha bacterial diversity within the nostril, tonsil, and lung samples was observed, indicating distinct bacterial compositions among sampling time points. Oral probiotic treatment did not change alpha diversity in any respiratory tissue, however, spatial variability in bacterial taxa composition was observed among the three respiratory tract regions. While the majority of differentially abundant taxa in probiotic treated calves were unique to their anatomical location, a few were common to two anatomical locations and one Finegoldia amplicon sequence variant was differentially abundant in all three anatomical locations.DiscussionIn conclusion, these findings contribute to the understanding of the dynamic nature of bacterial diversity and the potential effects of probiotics within the bovine respiratory tract and provides insight for future studies of probiotics on animal health, disease prevention, and management.
Deciphering of differences in gut microbiota and plasma metabolites profile between non-obese and obese Golden Retrievers dogs
Authors: Cai Yazhen , Tang Huayu , Xiang Guilin , Yi Hongyu , Zhong Jie , Xie Zhaoxi , Hu Qinfeng , El Bouhi Romaissaa , Zhou Pan , Zhang Yong , Yan Honglin 
Published: January 2025

IntroductionGolden Retrievers have a high risk of obesity, which is prevalent in dogs and is associated with inflammation and cancer, impairing the health and life expectancy of companion animals. Microbial and metabolite biomarkers have been proposed for identifying the presence of obesity in humans and rodents. However, the effects of obesity on the microbiome and metabolome of Golden Retrievers remains unknown. Therefore, this study was designed to evaluate the signatures of serum biochemistry indexes, gut microbiota and plasma metabolites in non-obese and obese Golden Retrievers, aiming to recognize potential biomarkers of canine obesity.MethodsA total of 8 non-obese (Ctrl group) and 8 obese (Obe group) Golden Retrievers were included in the present study to collect blood and feces samples for measurements. The fecal microbiome and plasma metabolome were determined using 16S rRNA amplicon sequencing and liquid chromatography-mass spectrometry, respectively.ResultsResults showed that the alanine aminotransferase activity and total bilirubin concentration, which have been measured using serum biochemistry analysis, were higher in the Obe group than in the Ctrl group (p &lt; 0.05). Moreover, there was a significant difference in gut microbiota composition between the two groups (p &lt; 0.05). The phyla Proteobacteria, Fusobacteriota, and Bacteroidota as well as genera Fusobacterium, Prevotella, Faecalibacterium, Escherichia-Shigell, and Alloprevotella were more abundant, while phylum Firmicutes and genera Peptoclostridium, Blautia, Turicibacter, Allobaculum, and Erysipelatoclostridium were less abundant in the Obe group compared to the Ctrl group (p &lt; 0.05). Plasma concentrations of citrulline and 11-dehydrocorticosterone were significantly higher in the Obe group than those in the Ctrl group (p &lt; 0.05). Close correlations between serum biochemistry parameters, gut microbiome, and plasma metabolites were observed in the current study.ConclusionThe obesity-induced shifts in serum biochemistry indexes, gut microbiota, and plasma metabolites profiles suggest that obese Golden Retrievers exhibit a different microbiome and metabolome than non-obese ones, and the certain metabolites like citrulline and 11-dehydrocorticosterone could be considered as potential biomarkers to recognize obese Golden Retrievers.
Trends and advances in Leptospira, a bibliometric analysis
Authors: Wang Wei , Gao Yamin , Ji Jianyu , Huang Zhai , Xiong Bin , Xiang Shulin 
Published: January 2025

BackgroundLeptospirosis is an acute zoonotic disease caused by pathogenic Leptospira, primarily transmitted to humans through contact with water or soil contaminated by the bacteria. It is globally distributed, with heightened prevalence in tropical regions. While prior studies have examined the pathophysiology, epidemiology, and risk factors of leptospirosis, few have explored trends and emerging topics in the field. This study applies bibliometric analysis to generate a visual knowledge map, identifying research hotspots and forecasting future trends in leptospirosis investigations.MethodsData were extracted from the Web of Science Core Collection (WOSCC), encompassing all publications up to May 1, 2024. CiteSpace and VOSViewer software were used to analyze annual publication trends, as well as contributions from countries, institutions, journals, authors, references, and keywords, thereby pinpointing current research priorities and potential future directions.ResultsA total of 5,244 articles were included, sourced from 4,716 institutions, 955 journals, and 156 countries or regions. The United States led with 1,315 publications and had the most significant influence in the field. “PLOS Neglected Tropical Diseases” published the highest number of articles (166), while “Infection and Immunity” garnered the most citations (6,591). Prominent research areas included restriction endonucleases, monoclonal antibodies, outer membrane proteins, water environments, detection methods, and antimicrobial agents. Research focus has shifted from early genomic and antigenic studies to investigations into outer membrane protein functions and environmental persistence, culminating in recent advances in molecular mechanisms and diagnostic technology development.ConclusionThis bibliometric analysis provides a comprehensive snapshot of leptospirosis research, emphasizing collaborations and impact among authors, countries, institutions, and journals. It offers valuable insights into ongoing trends and serves as a reference for future collaboration and research opportunities in the field.
Hotspots and research trends of gut microbiome in polycystic ovary syndrome: a bibliometric analysis (2012–2023)
Authors: Wu Ruishan , Mai Zhensheng , Song Xiaoyan , Zhao Wenzhong 
Published: January 2025

IntroductionPolycystic ovary syndrome (PCOS) is a common gynecological condition affecting individuals of reproductive age and is linked to the gut microbiome. This study aimed to identify the hotspots and research trends within the domain of the gut microbiome in PCOS through bibliometric analysis.MethodsUtilizing bibliometric techniques, we examined the literature on the gut microbiome in PCOS from the Web of Science Core Collection spanning the period from 2012 to 2023. Analytical tools such as CiteSpace, VOSviewer, and Bibliometric R packages were employed to evaluate various metrics, including countries/regions, institutions, authors, co-cited authors, authors’ H-index, journals, co-references, and keywords.ResultsA total of 191 publications were identified in the field of gut microbiome in PCOS, with an increase in annual publications from 2018 to 2023. People’s Republic of China was the most productive country, followed by the United States of America (USA), India. Shanghai Jiao Tong University, Fudan University, and Beijing University of Chinese Medicine were the top three most publications institutions. Thackray VG was identified as the most prolific author, holding the highest H-index, while Liu R received the highest total number of citations. The journal “Frontiers in Endocrinology” published the most articles in this domain. The most frequently co-cited reference was authored by Qi XY. The analysis of keyword burst detection identified “bile acids” (2021–2023) as the leading frontier keyword. Additionally, “gut dysbiosis,” “phenotypes,” “adolescents,” “metabolomics,” “metabolites,” “fecal microbiota transplantation,” and “IL-22” have emerged as the primary keywords reflecting recent research trends.ConclusionThis bibliometric analysis explores how the gut microbiome influences endocrine and metabolic disorders related to PCOS, emphasizing its role in the development of PCOS and treatments targeting the gut microbiome. The findings serve as a valuable resource for researchers, enabling them to identify critical hotspots and emerging areas of investigation in this field.
Decreased prefrontal activation during verbal fluency task after repetitive transcranial magnetic stimulation treatment for depression in Alzheimer’s disease: a functional near-infrared spectroscopy study
Authors: Zhao Yuanzhi , Qiu Conglong , Lin Ping , Yang Mei , Huang Ling , Zhao Zheng , Wu Xiangping , Zhou Dongsheng 
Published: January 2025

BackgroundStudies have shown the clinical effects of repetitive transcranial magnetic stimulation (rTMS) on depression in Alzheimer’s disease (AD). However, the underlying mechanisms remain poorly understood. The measurement of brain activation links neurobiological and functional aspects but is challenging in patients with dementia. This study investigated the influence of rTMS on cortical activation in patients with AD and depressive symptoms, measured using functional near-infrared spectroscopy (fNIRS) during a verbal fluency task.MethodsIn this randomized, double-blind study, patients with AD and depression received either active rTMS (n = 17) or sham-rTMS (n = 16). Patients received 4 weeks of bilateral standard rTMS (1 Hz rTMS delivered to the right dorsolateral prefrontal cortex (DLPFC) and 10-Hz rTMS delivered to the left DLPFC).ResultsNo significant changes were found in the Mini-Mental State Examination (MMSE) and Modified Barthel Index (MBI); however, significant changes were found for the 17-item Hamilton Depression Rating Scale (HAMD-17) and the depression score of the Neuropsychiatric Inventory (NPI-depression; p &lt; 0.05). The results showed a decrease in the concentration of oxygenated hemoglobin, as measured with fNIRS, from baseline to week 4 in CH41 (in right DLPFC; p = 0.0047, FDR-corrected). There was a negative correlation between the improvement in HAMD-17 severity in these patients and reduced oxygenated hemodynamic response of CH41 (r = − 0.504, p = 0.039).ConclusionThe results indicated a positive effect of rTMS on depression in patients with AD. The underlying cortical changes were imaged using fNIRS. Prefrontal activation measured by fNIRS is a potential biomarker for monitoring the response of patients with depression in AD to rTMS treatment.