bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a multi-dimensional health evaluation index system for occupational population from the perspective of health ecology: A Delphi study
Authors: Zhou Xiaoling , Wu Jing , Liu Ying , Zhou Geyao , Yan Yuzhong , Li Ming 
Published: August 2023

Abstract

BackgroundThe health status of occupational population is very critical to the development of countries and regions as the main force of social and economic development. However, there is a dearth of comprehensive and systematic indicators to evaluate for the health of occupational groups at present. This paper aims to construct a multi-dimensional evaluation index system for occupational population.methodsThis study combined literature review and initially established a multidimensional health system framework for occupational population based on health ecology theory, and then used two rounds of Delphi expert consultation to construct the final multidimensional health index system for occupational population. The study selected 15 experts in related fields for two rounds of Delphi expert consultation. The index selection criteria were the average importance score >4 and the coefficient of variation <0.25.ResultsThe recovery rates of the two rounds of expert questionnaires were 100.00% and 93.33% respectively, the expert authority coefficient were 0.90, and the Kendall’s coordination coefficients of the first and second level indexes were 0.32 and 0.42 (P≥ 0. 001). The final index system includes 4 primary indicators (individual characteristics, health knowledge, health behavior and health skills), 13 second-level indicators and 41 third-level indicators.The weight coefficients of the four primary health dimensions are relatively close, and the "health knowledge" is slightly higher.ConclusionsThe multi-dimensional health index system of occupational population established in this study is comprehensive, reliable and reasonable, and can be used as a reference tool for comprehensive evaluation of health status of general occupational population.
Mitoguardin-2 promotes cell proliferation by activating AKT and regulating Hippo/YAP1 signaling pathway in ovarian granulosa cells
Authors: Liu Xiao-Man , Yan Ming-Qi , Zhu Bing-Hong , Liu Xiao-Hong , Yang Yu-Meng , Duan Xiu-Yun , wang Yong , Sun Hui , Feng Mei 
Published: August 2023

Abstract
Mitochondria have been identified to be involved in oxidative phosphorylation, lipid metabolism, cell death, and cell proliferation. Previous studies have demonstrated that Mitoguardin (Miga), a mitochondrial protein that governs mitochondrial fusion, mitochondria-ER contacts, lipid formation and autophagy, is crucial for ovarian endocrine and follicular development. Nevertheless, it remains uncertain whether Miga is implicated in the regulation of ovarian granulosa cell proliferation, which cellular signals are involved, and how they are regulated. This study revealed that mammalian MIGA1,-2 promotes cell proliferation and regulates the phosphorylation and localization of YAP1 in ovarian granulosa cells. The upregulation of MIGA2 resulted in a reduction of YAP1 activity, while the removal of MIGA2 led to an increase in YAP1 activity. Further analyses have indicated that MIGA1,-2 regulated YAP1 via the Hippo signaling pathway and regulate AKT activity in collaboration with YAP1. In addition, LPA was able to regulate MIGA2 expression and AKT activity by activating YAP1. In brief, our findings demonstrate that the mitochondrial protein MIGA2 regulates the Hippo/YAP1 signaling pathway and promotes AKT activity and cellular proliferation in ovarian granulosa cells, which may contribute to the molecular pathogenesis of reproductive endocrine disease such as polycystic ovary syndrome.
Decomposition analysis of Antenatal care utilization inequities among underprivileged communities in southern Ethiopia
Authors: Mekango Dejene Ermias , Moges Sisay , Lajore Bereket Abrham , Buda Alula Seyum , Ejajo Tekle , Erkalo Desta 
Published: August 2023

Abstract
Background
Health equity has emerged as a global issue in the post-2015 Sustainable Development Goals, and Ethiopia is no exception. Despite positive improvements, inequities in maternal health service utilization among demographic groups continue to be one of Ethiopia's significant challenges. This study focuses on antenatal care service discrimination among a local underprivileged group of women known as the "Golden Hands" community in Ethiopia's Kembata Tembaro Zone. The subgroup community consists of underprivileged women “Golden-hands," formerly known as "Fuga," who face discrimination in all aspects of life owing to their living conditions and ethnic background.
Methods
A community-based comparative cross-sectional study was conducted in the Kembata-Tembaro zone of Southern Ethiopia. Double population proportion sample size estimation method was used and a total of 1,210 eligible participants were included in the study, out of which 440 of were Golden-Hands community members. Stratified random sampling was used to choose the samples. The concentration curve and index, as well as multivariable decomposition analysis, were used to examine inequality.
Result
Discrimination accounted for 60.23 percent of the decreased ANC service use by the Golden Hand community and Endowment accounts for just 39.47 percent of the reduced ANC service. The rate of ANC service is 3.64 times greater in women in the top quintile than in those in the lowest. increasing in age, urban residence, and wealth index were the most important independent factors with statistically significant contributions to discriminated difference.
Conclusion
High disparity in ANC service was observed in the study and, residency and wealth index contribute the highest proportion to the inequity. Therefore, marginalized groups in society people should get higher attention by policymakers and other organization.
The anti-proliferative potential of Albizia lebbeck ethanolic extract on human breast cancer cells and performance of extreme machine models in modelling of cell migration
Authors: Umar Huzaifa , Usman Abdullahi Garba , Rabiu Maryam , Ghali Umar Muhammad , Abba Sani Isah , Ozsahin Dilber Uzun 
Published: August 2023

Abstract
Cancer is one of the major causes of death in the modern world, and the incidence varies considerably based on race, ethnicity, and region. Novel cancer treatments, such as surgery and immunotherapy, are ineffective and expensive. In this situation, ion channels responsible for cell migration have appeared to be the most promising targets for cancer treatment. This research presents findings on the organic compounds present in Albizia lebbeck ethanolic extracts (ALEE), as well as their impact on the anti-migratory, anti-proliferative and cytotoxic potentials on MDA-MB 231 and MCF-7 human breast cancer cell lines. In addition, artificial intelligence (AI) based models, multilayer perceptron (MLP), extreme gradient boosting (XGB), and extreme learning machine (ELM) were performed to predict in vitro cancer cell migration on both cell lines, based on our experimental data. The organic compounds composition of the ALEE was studied using gas chromatography-mass spectrometry (GC-MS) analysis. Cytotoxicity, anti-proliferations, and anti-migratory activity of the extract using Tryphan Blue, MTT, and Wound Heal assay, respectively. Among the various concentrations (2.5 ~ 200 µg/mL) of the ALLE that were used in our study, 2.5 ~ 10 µg/mL revealed anti-migratory potential with increased concentrations, and they did not show any effect on the proliferation of the cells (P < 0.05; n ≥ 3). Furthermore, the three data-driven models, Multi-layer perceptron (MLP), Extreme gradient boosting (XGB), and Extreme learning machine (ELM), predict the potential migration ability of the extract on the treated cells based on our experimental data. Overall, the concentrations of the plant extract that does not affect the proliferation of the type cells used demonstrated promising effects in reducing cell migration. XGB outperformed the MLP and ELM models and increased their performance efficiency by up to 3% and 1% for MCF and 1% and 2% for MDA-MB231, respectively, in the testing phase.
Sago Sep: A Traditional Food Source for the Marind Anim Tribe in Eastern Indonesia
Authors: Kadir Akhmad , Suharno Suharno , Reawaruw Yohana N.I. , Ali Aisyah 
Published: August 2023

Abstract
Food sources around the world depend on the local availability in nature. In Indonesia, one of the naturally and locally available food sources is sago, which is processed into various traditional foods to meet the community’s daily needs. Aside from fulfilling the demand for food, sago is also culturally valuable. This study examines sago processing into traditional cuisines in the southern part of Papua, Indonesia, with data collected through a survey. The results show that the indigenous Marind Anim people process sago into different traditional foods: papeda, roasted sago or sago plates, and sago sep. Sago sep is a traditional food of the Marind Anim tribe commonly found in the lowland areas of Merauke. Meanwhile, Papeda and sago plates are influenced by the migrant community consisting of the northern Papuan and Maollucan indigenous peoples. Sago Sep’s types are sago sep Teta, Komabo, Nggalamo, Ebayak, Kwetahuk, and Sinoli, distinguished based on the additional constituent ingredients.
Enhancing Deblocking Efficiency and Curing Reaction of Phenol-Blocked Polymeric Methylenediphenyldiisocyanate for Advanced Epoxy-Polyurethane Film Formulation
Authors: R Karthi , Govindarajan Sankar 
Published: August 2023

Abstract
Novel phenol blocked polymeric methylene diphenyldiisocyanate (pMDI) derivatives were synthesized and characterized using FTIR, 1H NMR, and 13C NMR spectroscopy. The deblocking temperatures of the blocked isocyanates were determined using CO2 evolution method, DSC, and TGA spectroscopy. A cure reaction was performed between the blocked isocyanates and poly(Terathane-2000) to establish the structure-property relationship. Unsubstituted phenol-based blocked isocyanates exhibited superior deblocking temperatures and gel times compared to those with electron-donating substituted phenols. Intramolecular hydrogen bonding in blocking agents such as 4-hydroxybenzaldehyde, 4-hydroxy acetophenone, 4-hydroxy benzoic acid, and methyl 4-hydroxy benzoate resulted in unexpectedly high deblocking temperatures. Conversely, electron-withdrawing and steric effects led to low deblocking temperatures and gel times for blocked isocyanates BI-13 and BI-15. The solubility behavior of the blocked isocyanates was studied using various polyols (PEG-400, PEG-4000, and PEG-6000). Epoxy-polyurethane films were prepared using the blocked isocyanates, and their structures were confirmed using ATR spectroscopy. The thermal and mechanical properties of these films were investigated, including shoreA hardness, tensile strength, and flexural strength.
Trends in deaths and disability-adjusted life-years of stroke attributable to low physical activity worldwide, 1990-2019
Authors: Li Jun-xiao , Zhong Qiong-qiong , Yuan Shi-xiang , Zhu Feng 
Published: August 2023

Abstract
Background
Low physical activity (LPA) has been linked to the risk of stroke. Previous studies on the disease burden of stroke attributable to LPA are incomplete and lagged. We aim to assess spatiotemporal trends in the global burden of stroke attributable to LPA from 1990 to 2019.
Methods
Based on the global burden of Disease, Injuries, and Risk Factors Study, our research examined deaths, the Disability-Adjusted Life Years (DALYs), the Age-Standardized Mortality Rate (ASMR), the Age-Standardized DALY Rate (ASDR), and the Estimated Annual Percentage Change (EAPC) for stroke attributable to LPA.
Results
Deaths and DALYs were on the rise worldwide from 1990 to 2019, with increases of 72.72% for the former and 67.41% for the latter; ASMR and ASDR decreased, with the ASMR-related EAPC of -1.61 (95% CI:-1.71–-1.5) and ASDR-related EAPC of -1.35 (95% CI:-1.43–-1.27); the females had more numbers of deaths and DALYs, and the majorities of deaths and DALYs were shared by those aged ≥70. The highest-burden rates were shared by North Africa, Middle East, and Tropical Latin America; the ASMR-related EAPC was associated with the ASMR in 1990 (R=-0.26, P<0.001) and the Socio-Demographic Index (SDI) across different countries in 2019 (R=-0.61, P<0.001), respectively, and such patterns were similar to what ASDR and the ASDR-related EAPC had; the Human Development Index (HDI) in 2019 was associated with the ASMR-related EAPC (R=0.63, P<0.001) and the ASDR-related EAPC across different countries (R=-0.62, P< 0.001), respectively.
Conclusions
Globally, deaths and DALYs of stroke attributable to LPA have deteriorated over the recent three decades. Special attention should be given to the effects of physical activity on health, and patients with stroke attributable to LPA worldwide should energetically be cared about, especially among those aged ≥ 70 and females in the regions of East Asia, North Africa, and the Middle East.
Walk or be walked by the dog? The attachment role
Authors: Martins Catarina Freitas , Silva Luís , Soares Jorge P. , Pinto Graça S. , Abrantes Catarina , Cardoso Luís , Pires Maria A. , Mota Maria P. 
Published: August 2023

Abstract
Background The human-animal bond has been recognized as having positive effects on the health and well-being of both humans and pets. The present study aims to explore the influence of attachment on physical activity (PA), lifestyle, and health outcomes of dog owners (DO), highlighting the mutual benefits resulting from the relationship between DO and dogs.Methods Thirty-eight DO and their dogs participated in this study. Socio-demographic data, the Subjective Perceptive Health Scale (SPHS), FANTASTIC lifestyle, and the Lexington Attachment Pet Scale (LAPS) were assessed. PA was measured in both the DO and the dogs, using an ActiGraph GT3X accelerometer in the context of daily routine. Descriptive statistics and Spearman rank correlation analyses were performed to examine the associations between LAPS, PA levels, variables socio-demographic, lifestyle behaviors, and SPHS.Results Significant correlations were found between the DO’s light-level PA and the pets' vigorous level of PA (rho = 0.45). Furthermore, the importance of the pets' health and the LAPS subscales, namely proximity and attachment, were related to taking the pet for a walk. Concerning lifestyle, DO with a healthier lifestyle have a better self-assessment of their health using the SHPS (rho = 0.39, p = 0.02). Moreover, DO with better lifestyles have also exhibited greater concern for their pet's health and had higher levels of PA.Conclusions This study underlines that individuals adopting healthier habits tend to perceive themselves as healthier and exhibit greater concern for their pets' health. The attachment between DO and dogs is important to promote healthy lifestyle behaviors and PA engagement. Our results highlight that the presence of a dog is associated with a higher level of DO’s PA, depending on the strength of the human-animal bond.
Illustration of association between change in prostate-specific antigen (PSA) values and time to tumor status after treatment for prostate cancer patients: A joint modelling approach
Authors: Liaqat Madiha , Kamal Shahid , Fischer Florian 
Published: August 2023

Abstract
Background
Biomarkers’ information utilize for prediction of new patient’s suffering using statistical approaches to model outcomes. Simultaneous analysis of prostate-specific antigen (PSA) and time to tumor shrinkage after treatment allows it to make dynamic prediction adjustable over time. The motivation of this work is to elaborate the dynamic prediction about individual-specific trajectories of prostate cancer progression and tumor shrinkage, using baseline patients’ characteristics and longitudinal PSA data.
Methods
We developed joint models to predict low tumor status after treatment considering repeated PSA measurements, adjusted for baseline body-mass-index. We modeled 1,504 prostate cancer patients’ data jointly using mixed-effect sub-model for longitudinal PSA and an event time sub-model for tumor shrinkage.
Results
Baseline prognostic factors affected PSA level over time; also, an association of PSA on time with tumor status is evaluated. Patients who observed low tumor status had low PSA values during follow-up and also had exponentially decreasing PSA within one month after treatment. Factors included in this analysis were significant in mixed-effects and event time sub-models.
Conclusions
Medical science has evolved from subjective prediction to dynamic prediction, based on statistical knowledge with use of joint modelling of longitudinal data and event time outcomes. It provides individual event prediction with dynamic nature of time-accumulated evidence-based tools. We developed a joint model to predict prostate cancer patients’ tumor status after treatment with observing longitudinal PSA. It is evaluated that dynamically updated PSA information can improve prognosis, which is used to make follow-up and treatment decision-making easy and fruitful.
Multi-omics Analysis of Tumor Necrosis Factor Superfamily 4 Reveals Its Prognostic Value and T Cell Exhaustion Feature in Cancer
Authors: Luo Tianqi , Wu Yue , Chen Zewei , Tai Yi , Zhang Feiyang , Chen Weiqing , Lei Kai , Zhou Qi 
Published: August 2023

Abstract
Background
As a member of the Tumor Necrosis Factor (TNF) family, Tumor Necrosis Factor Superfamily 4 (TNFSF4) participated in various immune-related processes. Nevertheless, much is yet to be elucidated regarding the biological function of TNFSF4 in pan-cancer.
Methods
RNA-seq and corresponding clinical variables were gained from the Cancer Genome Atlas (TCGA). Immunotherapy cohorts were retrieved and downloaded from Gene Expression Omnibus (GEO). Tumor Immune Estimation Resource (TIMER) was used to evaluate tumor-infiltrating immune cell level in tumors. The Tumor Immune Single-cell Hub 2 (TISCH2) database was utilized to examine the expression of TNFSF4 across various tumor cell subsets. Gene Set Enrichment Analysis (GSEA) was utilized to explore related signaling pathways of TNFSF4.
Results
We utilized bioinformatic methods to analyze the TNFSF4 across TCGA cancers and found that TNFSF4 expression was highly increased in cancers compared to normal tissues. Further immunohistochemistry staining of multiple tumors validated this finding. Univariate Cox regression and survival analyses revealed that TNFSF4 served as a risk factor for a majority of cancer. Furthermore, TNFSF4 expression increased with the progression of tumor stage in multiple cancers. In the two immunotherapy cohorts from the GEO database, the proportion of PR/CR patients was higher in the TNFSF4 low-expression group. Through integrating single-cell data from the TISCH database, we found that TNFSF4 expression was predominantly observed in proliferative T cells and exhausted T cells. Correlation analysis demonstrated that TNFSF4 was positively associated with a range of immunomodulatory genes. Ultimately, analysis of GSEA revealed that TNFSF4 was related to immune response and EMT pathways.
Conclusions
We identified the function of TNFSF4 as a valuable molecule for cancer, and its capacity to predict response of immunotherapy. Importantly, our work revealed a robust association between TNFSF4 and exhausted T cells within the tumor microenvironment, which indicated TNFSF4 may be a promising biomarker for cancer treatment.
A Prospective Comparative Study on Bladder Volume Measurement in Patients with Pelvic Tumor Undergoing Radiotherapy: Portable Bladder Scanner from China versus CT Analog Positioning Machine
Authors: Bai fei , Hu Qiu Xia , Yao xiao wei , CHENG ming , Zhao lina , lin 许林林 Xu lin 
Published: August 2023

Abstract
Objective
The purpose of this study was to investigate whether the bladder volume of patients with pelvic tumor treated with radiotherapycan be accurately measured using the Meike Palm Bladder Scanner PBSV3.2 manufactured in China and the accuracy of its measurement under different influencing factors.
Methods
To compare the consistency of treatment planned CT bladder volume and bladder volume measured by PBSV3.2 in 165 patients with pelvic radiotherapy tumors. To investigate the accuracy of PBSV3.2 in different sex, age, treatment purpose, and bladder volume.
Results
There was a significant positive correlation with bladder volume on CT and PBSV3.2 (r=0.874; p<0.001). The mean difference between CT measured values and PBSV3.2 was (-0.14±50.17) ml. The results of the different variables showed that the overall mean of PBSV3.2 and CT measurements were statistically different in the age ≥65 years, bladder volumes >400ml and ≤400ml groups (p = 0.028, 0.002, 0.001). There was no statistical significance between the remaining variables. The volume difference between PBSV3.2 measurement and CT was 12.87ml in male patients, which was larger than that in female patients 3.27ml. Pearson correlation analysis showed that the correlation coefficient was 0.473 for bladder volume greater than 400ml and 0.868 for bladder volume less than 400ml; the correlation coefficient of the other variables ranged from 0.802 to 0.893.
Conclusion
This is the first large-sample study to evaluate the accuracy of PBSV3.2 in a pelvic tumor radiotherapy population using the convenient bladder scanner PBSV3.2 made in China. PBSV3.2 provides an acceptable indicator for monitoring bladder volume in patients with pelvic radiotherapy. It is recommended to monitor bladder volume with PBSV3.2 when the planned bladder volume is 200-400ml. For male and patients ≥65 years old, at least two repeat measurements are required when using a bladder scanner and the volume should be corrected by using a modified feature to improve bladder volume consistency.
Andes virus mRNA vaccines: comparison of unmodified and modified mRNA platforms
Authors: Bukreyev Alexander , Kuzmin Ivan , Acosta Ruben Soto , Wasdin Perry , Mire Chad , Engdahl Taylor , Moon Woohyun , Popov Vsevolod , Crowe James , Georgiev Ivelin , Garcia-Blanco Mariano , Abbott Robert 
Published: August 2023

Abstract

Andes virus (ANDV) is a rodent-borne zoonotic orthohantavirus endemic in South America that causes hantavirus pulmonary syndrome in humans, with up to a 40% case fatality rate. We developed ANDV mRNA vaccines based on the M segment of the viral genome that codes for glycoproteins Gn and Gc in a single open reading frame of glycoprotein precursor (GPC). We generated RNAs either with regular uridine (U-mRNA) or N1-methylpseudouridine (m1Ψ-mRNA). Mice immunized by either ANDV U-mRNA or m1Ψ-mRNA developed similar germinal center responses in lymph nodes. Single cell RNA and BCR sequencing of germinal center B cells from vaccinated mice demonstrated similar levels of activation, except an additional cluster of cells exhibiting strong interferon response that was present in animals vaccinated with U-mRNA but not m1Ψ-mRNA. Furthermore, similar immunoglobulin class-switching and somatic hypermutations were observed for the two vaccines. Golden Syrian hamsters were immunized intramuscularly with 2 doses of the vaccines on days 0 and 21. The titers of Gn/Gc-binding antibodies were moderately greater for U-mRNA construct than for m1Ψ-mRNA construct, however, the titers of ANDV-neutralizing antibodies were equivalent. Vaccinated animals were challenged with a lethal dose of ANDV at 21 days after the boost, along with the naïve control group. All control animals succumbed to infection whereas all vaccinated animals survived without any detectable disease or viral load. The data demonstrate the development of effective vaccines against ANDV and the lack of a significant effect of m1Ψ mRNA modification on immunogenicity and protection in the hamster model.