bluuebunny/crossref_metadata_2025_split huggingface dataset:
Manufacturing Processes of Implantable Microelectrode Array for In Vivo Neural Electrophysiological Recordings and Stimulation: A State-Of-the-Art Review
Authors: Yi Dongyang , Yao Yao , Wang Yi , Chen Lei 
Published: August 2023

Abstract
Electrophysiological recording and stimulation of neuron activities are important for us to understand the function and dysfunction of the nervous system. To record/stimulate neuron activities as voltage fluctuation extracellularly, microelectrode array (MEA) implants are a promising tool to provide high temporal and spatial resolution for neuroscience studies and medical treatments. The design configuration and recording capabilities of the MEAs have evolved dramatically since their invention and manufacturing process development has been a key driving force for such advancement. Over the past decade, since the White House Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative launched in 2013, advanced manufacturing processes have enabled advanced MEAs with increased channel count and density, access to more brain areas, more reliable chronic performance, as well as minimal invasiveness and tissue reaction. In this state-of-the-art review paper, three major types of electrophysiological recording MEAs widely used nowadays, namely, microwire-based, silicon-based, and flexible MEAs are introduced and discussed. Conventional design and manufacturing processes and materials used for each type are elaborated, followed by a review of further development and recent advances in manufacturing technologies and the enabling new designs and capabilities. The review concludes with a discussion on potential future directions of manufacturing process development to enable the long-term goal of large-scale high-density brain-wide chronic recordings in freely moving animals.
Variability of Kinetic Response Estimates of Froude Scaled DeepCwind Semisubmersible Platforms Subjected to Wave Loading
Authors: Wisudawan Agro , Jaksic Vesna , Pakrashi Vikram , Murphy Jimmy 
Published: August 2023

Abstract
Froude scaling for Floating Offshore Wind Turbine (FOWT) platforms is typical for understanding and interpreting their behavior and subsequent designs for testing in wave basins. Despite its popularity, the variability and uncertainty of the kinetic responses of such floating structures as a function of scaling require more attention. This work addresses the question of consistency of Froude scaling by comparing the hydrodynamic responses of a range of DeepCwind semisubmersible FOWT scaled models (full model, 1/2, 1/4, 1/9, 1/16, 1/25, 1/36, 1/49, and 1/50). The comparison was made both in the mooring-line tension and bending moment of structural members, which are directly related to their safety limit states. Hydrodynamic forces due to diffraction, radiation, and viscosity along with hydrostatic forces and mooring boundaries are modeled by ansys-Aqwa, which were subsequently converted to bending moment estimates. The variability of kinetic responses like mooring-line tensions and bending moment estimates was investigated for each scaled model, along with the identification of regions of inconsistencies. In the context of offshore renewable energy development through technological readiness levels, the study is especially pertinent for understanding how force variabilities and uncertainties are related to these kinetic responses of semisubmersible platforms.
Scattering of Long Waves by Freely Oscillating Submerged Plates
Authors: Chen Yongbo , Hayatdavoodi Masoud , Zhao Binbin , Cengiz Ertekin R. 
Published: August 2023

Abstract
We consider a horizontal, submerged plate in shallow water that is allowed to oscillate in the vertical direction due to the wave loads. The plate is attached to a linear spring and damper to control the oscillations. The focus of the study is on the transformation of the wave field by the submerged oscillating plate. To estimate energy scattering, wave reflection and transmission coefficients are determined from four wave gauges: two placed upwave and two placed downwave of the oscillating plate. The flow is governed by the nonlinear level I Green–Naghdi (GN) equations, coupled with the equations of the vertical oscillations of the plate. Time series of water surface elevation recorded at gauges upwave and downwave of the plate obtained by the GN model are compared with the available laboratory experiments and other data, and very good agreement is observed. Wave reflection and transmission coefficients are then determined for a range of involved parameters, including wave conditions (wavelength and wave height), initial submergence depth of the plate, plate length, and the spring-damper system attached to the plate. It is found that a submerged oscillating plate can have a remarkable effect on the wave field and that nonlinearity plays an important role in this wave–structure interaction problem. Discussion is provided on how the wave reflection and transmission coefficients vary with the wave conditions, plate characteristics, initial submergence depth, and spring-damper system properties.
Characterization and Deviation Analysis of a High-Flux Solar Simulator With Metal-Halide Lamps
Authors: Zhang Yuankun , Shaw Matthew , Ekman Ben , Brooks Geoffrey , Rhamdhani Muhammad Akbar , Guo Chunsheng 
Published: August 2023

Abstract
Utilizing highly concentrated solar power for thermochemical processing as one of the extraterrestrial in situ resource utilization (ISRU) applications has been highlighted as an essential technique to support deep-space exploration in the future. Multi-source high-flux solar simulators (HFSSs) are widely employed to provide stable irradiance for indoor solar thermal experiments. Meanwhile, numerical modeling that can characterize the radiation transport mechanisms within the solar thermal system has been developed for performance evaluation before field trials. However, significant differences between simulated and measured flux distributions were shown for existing models developed based on the Monte-Carlo ray-tracing (MCRT) method, which has been attributed to only one or two specific reasons. In this paper, we proposed a comprehensive analysis of the concentration characteristics of a 42 kW metal-halide lamp HFSS, developed at Swinburne University of Technology, considering the effect of five aspects. The flux distribution, uniformity, and vector distribution under different configurations were compared to quantify the influence of these factors on receiving irradiance. The suitable arc size, reflector shape, and reflector surface properties of the existing HFSS have also been numerically determined to improve the model and reduce the root mean square error (RMSE) for the lamp array from 38.2% to 8.3%. This research provides a potential pathway to numerically predict the radiation transfer performance of HFSSs and determine the suitable configuration for desired solar thermochemical applications.
Reverse genetics of parrot bornavirus 4 reveals a unique splicing of the glycoprotein gene that affects viral propagation
Authors: Komorizono Ryo , Fujino Kan , Kessler Susanne , Runge Solveig , Kanda Takehiro , Horie Masayuki , Makino Akiko , Rubbenstroth Dennis , Tomonaga Keizo 
Published: August 2023

ABSTRACT

Viruses can utilize host splicing machinery to enable the expression of multiple genes from a limited-sized genome. Orthobornaviruses use alternative splicing to regulate the expression level of viral proteins and achieve efficient viral replication in the nucleus. Although more than 20 orthobornaviruses have been identified belonging to eight different viral species, virus-specific splicing has not been demonstrated. Here, we demonstrate that the glycoprotein (G) transcript of parrot bornavirus 4 (PaBV-4; species
Orthobornavirus alphapsittaciforme
), a highly virulent virus in psittacines, undergoes mRNA splicing and expresses a soluble isoform termed sGP. Interestingly, the splicing donor for sGP is not conserved in other orthobornaviruses, including those belonging to the same orthobornavirus species, suggesting that this splicing has evolved as a PaBV-4-specific event. We have also shown that exogenous expression of sGP does not affect PaBV-4 replication or
de novo
virion infectivity. In this study, to investigate the role of sGP in viral replication, we established a reverse genetics system for PaBV-4 by using avian cell lines and generated a recombinant virus lacking the spliced mRNA for sGP. Using the recombinant viruses, we show that the replication of the sGP-deficient virus is significantly slower than that of the wild-type virus and that the exogenous expression of sGP cannot restore its propagation efficiency. These results suggest that autologous or controlled expression of sGP by splicing may be important for PaBV-4 propagation. The reverse genetics system for avian bornaviruses developed here will be a powerful tool for understanding the replication strategies and pathogenesis of avian orthobornaviruses.

IMPORTANCE
Parrot bornavirus 4 (PaBV-4) is the dominant cause of proventricular dilatation disease, a severe gastrointestinal and central nervous system disease among avian bornaviruses. In this study, we discovered that PaBV-4 expresses a soluble isoform of glycoprotein (G), called sGP, through alternative splicing of the G mRNA, which is unique to this virus. To understand the role of sGP in viral replication, we generated recombinant PaBV-4 lacking the newly identified splicing donor site for sGP using a reverse genetics system and found that its propagation was significantly slower than that of the wild-type virus, suggesting that sGP plays an essential role in PaBV-4 infection. Our results provide important insights not only into the replication strategy but also into the pathogenesis of PaBV-4, which is the most prevalent bornavirus in captive psittacines worldwide.
Rheological heterogeneities at the roots of the seismogenic zone
Authors: Volpe G. , Pozzi G. , Locchi M.E. , Tinti E. , Scuderi M.M. , Marone C. , Collettini C. 
Published: August 2023

Abstract
Although rheological heterogeneities are invoked to explain differences in fault-slip behavior, case studies where an interdisciplinary approach is adopted to capture their specific roles are still rare. In this work, we integrated geophysical, geological, and laboratory data to explain how rheological heterogeneities influence the earthquake activity at the roots of the seismogenic zone. During the 2016–2017 Central Italy sequence, following the major earthquakes, we observed a deepening of seismicity within the basement associated with a transient stress change. Part of this seismicity was organized in clusters of events, with similar sizes and waveforms. The structural study of exhumed basement rocks highlighted a heterogeneous fabric made of strong, quartz-rich lenses (up to 200 m) surrounded by a weak, interconnected phyllosilicate-rich matrix. Laboratory experiments simulating the main shock–induced increase in loading rate showed that the matrix lithology experienced an accelerating and self-decelerating aseismic creep, whereas the lens lithology showed dynamic instabilities. Our results suggest that the post–main shock loading rate increases favored accelerated creep within the matrix, which promoted, as a consequence, seismic instabilities within the lenses in the form of clustered seismicity. Our findings emphasize the strong connection between seismicity and the structural and frictional properties of the seismogenic zone.
Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U. 
Published: August 2023

Abstract

Purpose:
The TNT trial (NCT00532727) showed no evidence of carboplatin superiority over docetaxel in metastatic triple-negative breast cancer (mTNBC), but carboplatin benefit was observed in the germline BRCA1/2 mutation subgroup. Broader response-predictive biomarkers are needed. We explored the predictive ability of DNA damage response (DDR) and immune markers.

Experimental Design:
Tumor-infiltrating lymphocytes were evaluated for 222 of 376 patients. Primary tumors (PT) from 186 TNT participants (13 matched recurrences) were profiled using total RNA sequencing. Four transcriptional DDR-related and 25 immune-related signatures were evaluated. We assessed their association with objective response rate (ORR) and progression-free survival (PFS). Conditional inference forest clustering was applied to integrate multimodal data. The biology of subgroups was characterized by 693 gene expression modules and other markers.

Results:
Transcriptional DDR-related biomarkers were not predictive of ORR to either treatment overall. Changes from PT to recurrence were demonstrated; in chemotherapy-naïve patients, transcriptional DDR markers separated carboplatin responders from nonresponders (P values = 0.017; 0.046). High immune infiltration was associated with docetaxel ORR (interaction P values &lt; 0.05). Six subgroups were identified; the immune-enriched cluster had preferential docetaxel response [62.5% (D) vs. 29.4% (C); P = 0.016]. The immune-depleted cluster had preferential carboplatin response [8.0% (D) vs. 40.0% (C); P = 0.011]. DDR-related subgroups were too small to assess ORR.

Conclusions:
High immune features predict docetaxel response, and high DDR signature scores predict carboplatin response in treatment-naïve mTNBC. Integrating multimodal DDR and immune-related markers identifies subgroups with differential treatment sensitivity. Treatment options for patients with immune-low and DDR-proficient tumors remains an outstanding need. Caution is needed using PT-derived transcriptional signatures to direct treatment in mTNBC, particularly DDR-related markers following prior chemotherapy.
Targeted Nanofitin-drug Conjugates Achieve Efficient Tumor Delivery and Therapeutic Effect in an EGFRpos Mouse Xenograft Model
Authors: Huet Simon , Zeisser Labouebe Magali , Castro Rute , Jacquot Perrine , Pedrault Jessy , Viollet Sébastien , Van Simaeys Gaetan , Doumont Gilles , Larbanoix Lionel , Zindy Egor , Cunha António E. , Scapozza Leonardo , Cinier Mathieu 
Published: August 2023

Abstract
Adjusting the molecular size, the valency and the pharmacokinetics of drug conjugates are as many leverages to improve their therapeutic window, notably by affecting tumor penetration, renal clearance, and short systemic exposure. In that regard, small tumor-targeting ligands are gaining attention. In this study, we demonstrate the benefits of the small Nanofitin alternative scaffolds (7 kDa) as selective tumor-targeting modules for the generation of drug conjugates, focusing on Nanofitins B10 and D8 directed against the EGFR. Owing to their small size and monovalent format, the two Nanofitins displayed a fast and deep tumor penetration in EGFR-positive A431 xenografts in BALB/c nude mice after intravenous administration, yielding to a targeting of respectively 67.9% ± 14.1 and 98.9% ± 0.7 of the tumor cells as demonstrated by IHC. Conjugation with the monomethyl auristatin E toxin provided homogeneous Nanofitin-drug conjugates, with an overall yield of ≥97%, for in vivo assessment in a curative xenograft model using bioluminescent, EGFR-positive, A431 cells in BALB/c nude mice. Internalization was found critical for efficient release of the toxin. Hence, the intravenous administration of the D8-based construct showed significant antitumor effect in vivo as determined by monitoring tumor volumes and bioluminescence levels over 2 months.
Measurement and Management of Value Chain Greenhouse Gas Emissions from Supermarket Retailing
Authors: Mungkung Rattanawan , Nudchanate Tananon 
Published: August 2023

Abstract
This study quantified greenhouse gas emissions from indirect activities along the whole value chain of supermarket retailing to derive mitigation measures. Both direct and indirect greenhouse gas emission sources of a supermarket retailing value chain were identified and calculated using the national guidelines for estimating the carbon footprint for organizations, based on a total area of 13 248 m2 and operating 12 h per day. A scoring matrix was applied that considered the magnitude of emissions, the level of influence, and the risks or opportunities associated with business operations. The scoring results indicated a major contribution from value chain activities that should be included in any greenhouse gas analysis. The calculation revealed that the greenhouse gas emissions from the value chain activities were 33 784 t CO2 emitted yr−1 or 94% of total emissions. The key contributors were linked to the production of purchased goods and the management of food waste. Thus, value chain activities should not be overlooked in developing efficient greenhouse gas management strategies. Furthermore, purchased products and services carrying a carbon-reduction label should be given priority, and the application of artificial intelligence and innovation could be considered to reduce the amount of food waste from expired goods.
Minimally Invasive Hepatopancreatobiliary Surgery at a Large Regional Health System: Assessing the Safety of Program Expansion
Authors: Falls Samantha J. , Maxwell Conor M. , Kaye Dylan J. , Dighe Shruti G. , Schiffman Suzanne C. , Bartlett David L. , Wagner Patrick L. , Allen Casey J. 
Published: August 2023

Background Complex, minimally invasive hepatopancreatobiliary surgery (MIS HPB) is safe at high-volume centers, yet outcomes during early implementation are unknown. We describe our experience during period of rapid growth in an MIS HPB program at a large regional health system. Methods During an increase in MIS HPB (60% greater from preceding year), hospital records of patients who underwent HPB surgery between 1/1/2019 and 12/31/2020 were reviewed. Operative time, estimated blood loss (EBL), conversion rates, length of stay (LOS), and perioperative outcomes were assessed. Results 267 patients’ cases were reviewed. The population was 62 ± 13 years, 50% female, 90% white. MIS was more frequently performed for hepatic than pancreatic resections (59% vs 21%, P < .001). Open cases were more frequently performed for invasive malignancy in both pancreatic (70% vs 40%, P < .018) and hepatic (87% vs 70%, P = .046) resections. There was no difference in operative time between MIS and open surgery (293[218-355]min vs 296[199-399]min, P = .893). When compared to open, there was a shorter LOS (4[2-6]d vs 7[6-10]d, P < .001) and lower readmission rate (21% vs 37%, P = .005) following MIS. Estimated blood loss was lower in MIS liver resections, particularly when performed for benign disease (200[63-500]mL vs 600[200-1200]mL, P = .041). Overall 30-day mortality was similar between MIS and open surgery (1.0% vs 1.8%, P = 1.000). Discussion During a surgical expansion phase within our regional health system, MIS HPB offered improved perioperative outcomes when compared to open surgery. These data support the safety of implementation even during intervals of rapid programmatic growth.
Evaluation of subclinical cardiovascular risk in drug-naive pediatric patients with anxiety disorders
Authors: Akıncı Mehmet Akif , Uzun Necati , Alp Hayrullah 
Published: August 2023

Objective This study examined subclinical atherosclerosis in drug-naïve children with anxiety disorders using non-invasive measures to investigate the clinical features associated with subclinical atherosclerosis. Method A total of 37 drug-naive children and adolescents with anxiety disorders and 37 healthy controls were included in the study. The Children's Depression Inventory (CDI) and the State-Trait Anxiety Inventory (STAI-T and STAI-S) were used to assess children's depression and anxiety levels. Carotid artery intima-media (cIMT), epicardial adipose tissue (EAT), and periaortic adipose tissue (PAT) thicknesses, which are indicators of subclinical atherosclerosis, were obtained by echocardiographic measurements. Results Multivariate analysis of covariance (MANCOVA) revealed a significant main effect on cIMT, EAT thickness, and PAT thickness, independent of confounding factors such as age, sex, body mass index, mean blood pressure, and family income (Pillai's Trace V = .76, F (1, 72) = 35.60, P < .001, ηp2 = .76). Analysis of covariance (ANCOVA) showed that cIMT, EAT thickness, and PAT thickness values were significantly higher in the anxiety disorder group compared to the the control group ( P < .001). In partial correlation analysis, a positive correlation was observed between STAI-T and cIMT and EAT thickness. In linear regression analyses, age and STAI-T were significantly correlated with cIMT and EAT thickness levels. Conclusions These results suggest that subclinical cardiovascular risk is significantly increased in children and adolescents with anxiety disorders.
Health Economic Analysis of Antiviral Drugs in the Global Polio Eradication Endgame
Authors: Badizadegan Kamran , Kalkowska Dominika A. , Thompson Kimberly M. 
Published: August 2023

Background Polio antiviral drugs (PAVDs) may provide a critical tool in the eradication endgame by stopping poliovirus infections in immunodeficient individuals who may not clear the virus without therapeutic intervention. Although prolonged/chronic poliovirus excreters are rare, they represent a source of poliovirus reintroduction into the general population. Prior studies that assumed the successful cessation of all oral poliovirus vaccine (OPV) use estimated the potential upper bound of the incremental net benefits (INBs) of resource investments in research and development of PAVDs. However, delays in polio eradication, OPV cessation, and the development of PAVDs necessitate an updated economic analysis to reevaluate the costs and benefits of further investments in PAVDs. Methods Using a global integrated model of polio transmission, immunity, vaccine dynamics, risks, and economics, we explore the risks of reintroduction of polio transmission due to immunodeficiency-related vaccine-derived poliovirus (iVDPV) excreters and reevaluate the upper bound of the INBs of PAVDs. Results Under the current conditions, for which the use of OPV will likely continue for the foreseeable future, even with successful eradication of type 1 wild poliovirus by the end of 2023 and continued use of Sabin OPV for outbreak response, we estimate an upper bound INB of 60 million US$2019. With >100 million US$2019 already invested in PAVD development and with the introduction of novel OPVs that are less likely to revert to neurovirulence, our analysis suggests the expected INBs of PAVDs would not offset their costs. Conclusions While PAVDs could play an important role in the polio endgame, their expected economic benefits drop with ongoing OPV use and poliovirus transmissions. However, stakeholders may pursue the development of PAVDs as a desired product regardless of their economic benefits. Highlights While polio antiviral drugs could play an important role in the polio endgame, their expected economic benefits continue to drop with delays in polio eradication and the continued use of oral poliovirus vaccines. The incremental net benefits of investments in polio antiviral drug development and screening for immunodeficiency-related circulating polioviruses are small. Limited global resources are better spent on increasing global population immunity to polioviruses to stop and prevent poliovirus transmission.