bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cohort of live births from 2012 to 2020 in the municipality of Sao Paulo: identification of maternal, fetal, and newborn outcomes
Authors: Melo Tarcisio Cantos , Corrêa Fernanda Ferreira , Bonilha Eliana de Aquino , Aly Célia Maria Castex , Diniz Carmen Simone 
Published: May 2023

Abstract
The population’s health care may generate a large volume of data concerning services provided, making more knowledge available and making it possible to guide managers with respect to actions regarding health care. Among the databases processed by the Unified Health System [ Sistema Único de Saúde – SUS], are the System of Information on Live Births [Sistema de Informações sobre Nascidos Vivos (SINASC)], the System of Hospital Data [Sistema de Informações Hospitalares (SIH)] of the Ministry of Health, the System of Information on Mortality [Sistema de Informações de Mortalidade (SIM)], and, in the Municipality of Sao Paulo, the Integrated System of Health Care Management of the Municipal Department of Health [Sistema Integrado de Gestão da Assistência à Saúde da Secretaria Municipal de Saúde (SIGA)]. The objective of this study is to describe a method of linkage between the databases SIGA, SINASC, SIH and SIM making it possible to identify pregnant women/parturients and their fetuses and newborns in all these records. Consequently, analysis of the procedures and interventions undertaken on a woman during pregnancy and birth, those undertaken on her baby during and soon after birth, as well as hospitalizations, including those which occurred after discharge from hospital are attained. This study is part of a research project entitled, “How can interventions during labor and its outcomes become more visible to the data systems?”. The linkage of data from the SIGA, SINASC, SIH and SIM databases underwent 5 phases: treatment of the SIGA base, linkage, validations, descriptions, and metrics calculation.
Circular RNA Expression Profiles Are Significantly Altered after Cerebral Ischemia‒Reperfusion Injury in Different Parts of the Rat Brain
Authors: Xia Peishan , Yu Liying , He Bin , Pan Yuhua , Xu Jian , Wang Yijia , Yang Zhihua 
Published: May 2023

Abstract
Purpose
The objective of this study was to investigate the connection between circular RNA (circRNA) and ischemia-reperfusion (I/R) injury set on by middle cerebral artery occlusion (MCAO) in different parts of the rat brain.
Methods
Using the MCAO technique, the adult male Sprague-Dawley rat model was created. At 24 hours after reperfusion, circRNA sequencing and real-time PCR were used to assess the circRNA expression profile in the ischemic region of the right cortex, striatum, and hippocampus. A bioinformatics investigation of the changed circRNAs following I/R injury was carried out, including the discovery of miRNA binding sites, analysis of transcription factor binding, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and Gene Ontology (GO) analysis.
Results
85, 113 and 51 circRNAs in the cortex, striatum and hippocampus, respectively, substantially altered (|log2 (fold change)| > 1 and P < 0.05) after I/R, compared with the sham group. Significantly changed circRNAs found by sequencing analysis were confirmed by real-time PCR. Target genes of dysregulated circRNAs were connected to cellular components, biological processes, and molecular functions after I/R, according to GO and KEGG pathway studies. Finally, we successfully predicted a circMapk9-miR-29a-5p-gene network connected with NLRP3-mediated neuroinflammation in I/R.
Conclusion
The findings of this work provide the first investigation into the association between circRNAs and I/R injury brought on by MCAO in different parts of the rat brain, as well as a novel molecular target for the therapeutic management of I/R injury following endovascular thrombectomy (EVT).
Estimated enthalpy of formation of N-[3-(Dimethylamino)propyl] hexadecanamide by experimental approach
Authors: Foresto Luis Guilherme , Moya Silvia Fernanda , Suppino Raphael Soeiro 
Published: May 2023

Abstract

The N-[3-(Dimethylamino)propyl] hexadecanamide molecule is an important intermediate for synthesizing amphoteric and quaternary ammonium surfactants. Despite its importance for the household and personal care sectors, little to no information about its enthalpy of formation is found in the NIST database or literature, which hinders many industrial operations, especially its chemical production, by consuming much more energy than what is necessary. In this work, we present a first estimative of N-[3- (Dimethylamino)propyl] hexadecanamide's standard enthalpy of formation with a simple procedure that can be used to obtain similar parameters in chemical industry laboratories. The enthalpy estimative was obtained by reacting palmitic acid and dimethylaminopropylamine (DMAPA), yielding the desired compound. Hess's law was used to determine the enthalpy of the reaction through the heat associated with the extent of the reaction. The reagents were contacted in a simple calorimeter at room temperature (diluted with acetone) and 100 ºC (without acetone). The extent of the reaction was obtained by quantitative determination of the reaction medium in gas chromatography. The room temperature experiment led to an insignificant reaction extent which generated an unreliable result for the compound's enthalpy of formation. In contrast, at 100 ºC, the estimated standard enthalpy of the reaction was adequately calculated, leading to an enthalpy of formation of -703.93 ± 9.17 kJ/mol for the N- [3-(Dimethylamino)propyl] hexadecanamide. With this simple procedure, the industrial reactor's more precise heat consumption can be calculated, yielding economic and environmental benefits.
Strategies of positive deviants in fodder conservation among smallholder dairy farming systems in highlands and midlands of Kenya
Authors: Thomas Kogo , Yegon R. , Nthiwa D. , Migose S.A. 
Published: May 2023

Abstract

Fodder scarcity and low quality affect the productivity of dairy cattle in Kenya. Information on the unique strategies used by successful farmers also called positive deviants (PDs) among smallholder dairy farms is scanty. The objective of this study, therefore, was to determine the strategies distinguishing PDs from their peers (non-PDs), in fodder conservation at highlands and midlands agroecological zones of Tharaka Nithi County. Multistage sampling was used to select 121 smallholder dairy farms producing and conserving fodder. An increase in; land under fodder and crop residue, access to extension services, herd size, and milk yield (kg/cow/day) result in to increase in fodder conserved. Therefore, increased herd size, milk yield, land allocated to fodder production, and access to knowledge and skills promote fodder conservation. Further research on PDs methods and technologies of fodder conservation as well as economic performance could provide additional information to develop the dairy industry in highlands and midlands.
Mechanical Properties of SMA/PVA-ECC under Uniaxial Tensile Loading
Authors: yang zhao , Li Jiankun , Zhong Yilan , Qi Xiaolong 
Published: May 2023

Abstract
Although SMA/PVA hybrid fiber reinforced cementitious composites (SMA/PVA-ECC) exhibit excellent crack closure and deformation recovery capabilities, the research on their fundamental mechanical properties is still limited. This study investigates the tensile mechanical properties of SMA/PVA-ECC materials by conducting uniaxial tensile tests, analyzing the failure behavior, stress-strain curves, and characteristic parameters of the specimens, comparing the influence of SMA fiber content and diameter, and establishing a tensile constitutive model. The results show that the residual crack width of SMA/PVA-ECC specimens significantly decreases after unloading, and the SMA fiber content and diameter have a significant impact on the tensile properties of the specimens. The comprehensive tensile properties of specimens with a fiber diameter of 0.2 mm and content of 0.2% are the best, with their initial cracking strength, ultimate strength, and strain increasing by 56.4%, 23.6%, and 13.4%, respectively, compared to ECC specimens. The proposed bilinear tensile constitutive model has high accuracy. This study provides a theoretical basis for further research on SMA/PVA-ECC materials.
In-silico molecular characterization of DNAH6 and ATPase6 (Mitochondrial DNA) genes in asthenozoospermia patients in the northern region of India
Authors: Kumar Dinesh , Jeena Lalit Mohan , Tempe Anjali , Tanwar Renu , Kumar Suman 
Published: May 2023

Abstract

Background Male infertility due to spermatogenesis defects affects millions of men worldwide. However, the genetic etiology of the vast majority remains unclear. The present study was undertaken to assess the association of DNAH6 and ATPase6 genes in asthenozoospermia patients in the northern region of India.Methods A total of 60 semen samples were collected for the study, of which 30 were from the case group and 30 were from the control group. The semen samples for the case group (asthenozoospermia) and control groups were collected from IVF and Reproductive Biology Centre, Maulana Azad Medical College, New Delhi. Sperm count and motility were classified as per World Health Organization (WHO 2010) protocol. A total genomic DNA was extracted as per the stranded TRIZOL method with little modification.Results In-vitro molecular characterizations of DNAH6 and ATPase6 genes in both groups were checked by Polymerase Chain Reaction (PCR). The 675bp and 375bp amplicons were amplified using PCR for ATPase6 and DNAH6 genes. Our study results showed a significant (P ≤ 0.05) null deletion of DNAH6 and ATPase6 genes in asthenozoospermia patients as compared to the control. We found the significant null deletion of DNAH6 in case 45.0%, and the control group was 11.7%. However, in the case of APTase6, it was 26.7% and 10.0%, respectively.Conclusions Our study concluded that the presence of DHAH6 and ATPase6 genes had a significant impact on male infertility.
Increasing C-reactive protein levels in a patient with glioblastoma with lymph node metastasis. A case report
Authors: Kanemitsu Takuya , Furuse Motomasa , Kuwabara Hiroko , Yagi Ryokichi , Hiramatsu Ryo , Kameda Masahiro , Nonoguchi Naosuke , Kawabata Shinji , Takami Toshihiro , Arai Motohiro , Wanibuchi Masahiko 
Published: May 2023

Abstract
Background: Glioblastoma multiforme is an intractable brain tumor; however, extracranial metastases are rare. The sites of metastasis vary and include the lungs, bone, lymph nodes, and liver, with approximately half of patients with extracranial metastasis having metastasis in the lymph nodes. Most patients with extracranial metastases experience recurrence of the primary intracranial tumor. Lymph node metastases are often detected based on symptoms caused by other metastatic sites or lymphadenopathy. However, this case report describes a patient with glioblastoma with lymph node metastasis, whose C-reactive protein levels gradually increased prior to becoming febrile.
Case presentation: We reporta case of lymph node metastasis in glioblastoma multiforme that was difficult to diagnose because the patient had fever of unknown origin and the intracranial tumor did not recur. The patient was asymptomatic with a slowly increasing C-reactive protein levels without signs of infection, and he became febrile 9 months after the initial increase. The chest computed tomography performed supraclavicular, mediastinal, and hilarlymphadenopathy, and biopsy revealed lymph node metastasis of glioblastoma. This is the fifth case of lymph node metastasis without intracranial recurrence.
Conclusions: C-reactive protein levels may be a marker for the diagnosis of lymph node metastasis in patients with glioblastoma.
Assessment of Antioxidant activity of Silver Nanoparticles synthesized using aqueous extract of Schizophyllum commune inTriazophos-induced oxidative stress in albino rats
Authors: Kumar Amar , Haque Anam 
Published: May 2023

Abstract

Background: Several previous research works have reported that the silver nanoparticles have significant biofunctional role in wound healing, in drug-delivery system, catheter modification, bone healing and shows marked antibacterial, antioxidant, anti-inflammatory, anti-cancer, anti-viral and anti-fungal activities. Moreover the synthesis of silver nanoparticles by biological methods are ecofriendly and cost-effective. The present work has been aimed to synthesize and characterize the silver nanoparticles using aqueous extract of edible macrofungus Schizophyllum commune as well as to compare the antioxidant potentialities of the aqueous extract and the extract based silver nanoparticles.
Results: The silver nanoparticles were synthesized by the reduction of silver ions present in Silver Nitrate solution by the secondary metabolites present in the macrofungal extract which consequently produces a gradual color change in the solution. The synthesized silver nanoparticles showed the SPR (Surface Plasmon Resonance) peak at 431 in the UV-VIS spectrum. The SEM images showed that the nanoparticles have cuboidal and roughly spherical morphology with a size ranging from 80 nm to 110 nm, average size was found to be 96.13nm. The FT-IR spectrum revealed the surface coating of functional groups of different secondary metabolites present in the macrofungal extract. The results of in-vitroassessment of antioxidant activity revealed that the aqueous extract was found to have 21.28 % DPPH radical scavenging activity whereas the extract-based silver nanoparticles have 33.59 % DPPH radical scavenging activity at the same concentration of 100µg/ml. The in-vivo assessment of antioxidant activities of the extract and extract based silver nanoparticles involved the assessment of activity of key enzymes like CAT,SOD and GPx in the blood of albino rats and the results revealed that the extract and the extract based silver nanoparticles have significant antioxidant activities.
Conclusions: The synthesis of silver nanoparticles with S. commune extract is both environmentally freindly and cost-effective, and the nanoparticles were found to have a high level of stability. The antioxidant activity of the aqueous extract and extract-based silver nanoparticles against the DPPH radical was substantial. The aqueous extract of S. commune and extract-based silver nanoparticles were also found to have significant antioxidant activity during in vivo analysis using albino rat as animal model.
Identification of a Disulfidptosis-Related Gene Signature for Prognostic Implication in Colon Adenocarcinoma
Authors: Hu Gunchu , Yao Hongliang , Wei Zuxing , Li Linye , Yu Zhuowen , Li Jian , Luo Xiong , Guo Zhushu 
Published: May 2023

Abstract
We aimed to find genes related to disulfidptosis, a type of cell death that may affect cancer development. Disulfidptosis is a new research topic. We wanted to find genes related to disulfidptosis, a new type of cell death caused by excess cystine in cells. Disulfidptosis may affect cancer development differently from other types of cell death. We focused on colon adenocarcinoma (COAD), which is the second most deadly cancer worldwide and has been increasing rapidly in recent years. Tumor biomarkers can help diagnose and monitor tumors, so we need to find new ones for COAD and develop new treatments. We analyzed the mRNA expression data and clinical information of COAD patients from public databases. We used a machine-learning algorithm to select key features and build a signature. We found four genes that are involved in disulfidptosis: TRIP6, OXSM, MYH3 and MYH4. These genes can predict how long COAD patients will live. We also found that these genes affect the tumor microenvironment (TME), drug sensitivity and immune microenvironment of COAD patients. Our study shows that disulfidptosis-related genes are important for COAD prognosis and therapy. This is crucial for developing personalized cancer therapy.
Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3
Authors: Hamon Melanie , Chevalier Christine , Chica Claudia , Matheau Justine , Connor Michael , Pain Adrien 
Published: May 2023

Abstract

Epithelial cells are the first point of contact for bacteria entering the respiratory tract. Streptococcus pneumoniae is an obligate human pathobiont of the nasal mucosa, carried asymptomatically but also the cause of severe pneumonia. The role of the epithelium in maintaining homeostatic interactions or mounting an inflammatory response to invasive S. pneumoniae is currently poorly understood. However, studies have shown that chromatin modifications, at the histone level, induced by bacterial pathogens interfere with the host transcriptional program and promote infection. In this study, we demonstrate that S. pneumoniae actively induces di-methylation of lysine 4 on histone H3 (H3K4me2), which persists for at least 9 days upon clearance of bacteria with antibiotics. We show that infection establishes a unique epigenetic program affecting the transcriptional response of epithelial cells, rendering them more permissive upon secondary infection. Our results establish H3K4me2 as a unique modification induced by infection, distinct from H3K4me3, which localizes to enhancer regions genome-wide. Therefore, this study reveals evidence that bacterial infection leaves a memory in epithelial cells after bacterial clearance, in an epigenomic mark, thereby altering cellular responses for subsequent infection.
Local adaptation-induced evolutionary trap in alpine plants under climate change
Authors: Li Li , Zhang Jie , Sork Victoria , Fitzpatrick Matthew , Liao Hong , Wang Gang , Sun Yan-Bo , Li Qingjun , Zhao Jian-Li 
Published: May 2023

Abstract

Evolutionary traps occur when populations deviate from local adaptation and become maladapted under rapid climate change. Alpine plants, with genetically distinct ecotypes in sky islands, are susceptible to being trapped by rapid climate change. We employed landscape genomics to test a local adaptation-induced evolutionary trap in the alpine perennial plantRoscoea tibetica(Zingiberaceae) with three distinct ecotypes along altitudinal gradients in the Hengduan Mountains. Strong local adaptation to alpine environments was observed through isolation-by-environment and rapid decreases in effective population size following interglacial niche contraction. Genomic offset increased with climate warming and was positively correlated with the intensification of local adaptation. Instead of high-elevation ecotypes, an evolutionary trap was indicated in the low-elevation forest ecotype, which showed the strongest local adaptation and niche loss, requiring the greatest shift in allele frequencies to keep pace with future climate change, and will likely experience the largest population decline. Therefore, stronger local adaptation induces evolutionary traps more easily and aggravates the harm to alpine plants facing rapid climate warming. Our study provides the first empirical case of local adaptation-induced evolutionary traps for alpine plants and highlights that it is crucial for alpine biota conservation to consider and disarm evolutionary traps under climate change.
The Impact of Statin Dose, Class, and Intensity on Sepsis Mortality in Type 2 Diabetes Patients
Authors: Sun Mingyang , Tao Yuan , Chen Wan-Ming , Wu Szu-Yuan , Zhang Jiaqiang 
Published: May 2023

Abstract
Aims: The study aims to investigate the impact of statin use on sepsis mortality in patients with type 2 diabetes (T2DM) in a dose-, class-, and use intensity-dependent manner.
Methods: A retrospective cohort of 850,326 T2DM patients was analyzed using an inverse probability treatment-weighted Cox hazard model, with statin use status as a time-dependent variable.
Results: Results showed that statin use was associated with a significant reduction in sepsis mortality risk (aHR=0.29) compared to non-users. Pitavastatin, rosuvastatin, pravastatin, atorvastatin, simvastatin, fluvastatin, and lovastatin were all associated with a lower sepsis mortality risk. Higher cumulative defined daily dose per year of statins was also associated with a lower sepsis mortality, with an optimal daily dose of 0.86 defined daily doses.
Conclusion: These findings suggest that statin use may reduce sepsis mortality in T2DM patients and that higher doses are associated with greater protection.
Molecular detection of Coxiella burnetii infection in patients with infectious endocarditis culture negative after cardiovascular surgery in Vietnam
Authors: Duong Duc Hung , Do Thi Vinh An , Bui Thi Viet Ha , Vu Minh Tam 
Published: May 2023

Abstract
Objective: To detect Coxiella burnetii in endocarditis patients cultured negative for bacteria in a short-term study from January 2022 to February 2023 at Cardiovascular Surgery Unit, Bach Mai Hospital.
Materials and methods: 312 Patients with endocarditis diagnosed and operated at the Department of Cardiovascular Surgery - Bach Mai Hospital were included in the study after obtaining the consent of each study subject and the consent of the participants. hospital ethics committee. After surgery, the patient's blood sample was cultured to identify bacteria by an automatic system at the Department of Microbiology of Bach Mai hospital, 52 case was the result is negative, it will continue to be PCR analyzed with Coxiella burnetii primer pair at the Department of Microbiology, University of Natural Sciences, Vietnam National University, Hanoi.
Results: In a short study with 52 blood samples from endocarditis patients who were found to be negative for bacteria, 13samples were positive for Coxiella by PCR. Among 9 patients, there were 6 patients with fever lasting 14 days after surgery, 6patients with emaciation, pneumonia, 2patient with multi-organ failure, emaciation.
Pretreatment radiomics power in evaluating neoadjuvant chemotherapy response and outcome for patients with head and neck squamous cell carcinoma: a systematic review and meta-analysis
Authors: Yu Yalian , Li Xinyao , Wang Hongbo , Li Wei , Wang Yan 
Published: May 2023

Abstract
Background
This study conducted a meta-analysis of the evaluation value of radiomics in predicting induction chemotherapy (IC) response and prognosis of the patients with head and neck squamous cell carcinoma (HNSCC). By using the method of radiomic to formulate individualized treatment plans for the patients with HNSCC, the survival rate of the patients could be improved while maintaining the normal physiological functions.
Methods
We systematically searched the relevant articles on radiomics predicting IC response and prognosis of the patients with HNSCC published before June 1, 2022. Meta-analysis was used to evaluate the power for radiomics in predicting the response and prognosis of patients with HNSCC to IC.
Results
A total of 16 articles with 8238 patients were included in the study. The predictive value of IC response in patients with HNSCC showed that the diagnostic AUC was 0.87 (0.83–0.89), the sensitivity was 0.85 (0.69–0.94), and the specificity was 0.77 (0.65–0.86). By using radiomics, patients were divided into high-risk group and low-risk group, IC was the protective factor in the high-risk group. The survival time of overall survival (OS) in IC group was 0.35 (95% CI: 0.25–0.49) compared with the non-IC group, the disease progression free survival time (PFS) was 0.38 (95% CI: 0.28–0.52), and the metastasis free survival time (DMFS) was 0.37 (95% CI: 0.26–0.53).
Conclusions
Radiomics has a good predictive ability for the response of patients with HNSCC to IC and the impact of prognosis. Radiomics will assist clinicians in making more individualized treatment decisions for patients with HNSCC.