bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of TwoBactericeraSpecies (Hemiptera: Triozidae) With NativeLyciumspp. (Solanales: Solanaceae) in the Potato Growing Regions of the Rio Grande Valley of Texas
Authors: Cooper W Rodney , Esparza-Diaz Gabriela , Wildung Mark R , Horton David R , Badillo-Vargas Ismael E , Halbert Susan E 
Published: December 2022

AbstractBactericera cockerelli (Šulc) (Hemiptera: Triozidae) is a vector of ‘Candidatus Liberibacter solanacearum’ (Lso), the pathogen that causes potato zebra chip. Zebra chip incidence varies regionally, perhaps because of geographic differences in species of noncrop hosts available to the vector and in susceptibility of those hosts to Lso. Native and introduced species of Lycium (Solanales: Solanaceae) are important noncrop hosts of B. cockerelli in some regions of North America. Susceptibility of native Lycium species to Lso is uncertain. We investigated the use of two native species of Lycium by B. cockerelli in South Texas and tested whether they are susceptible to Lso. Bactericera cockerelli adults and nymphs were collected frequently from L. berlandieri Dunal and L. carolinianum Walter. Greenhouse assays confirmed that B. cockerelli develops on both species and showed that Lso infects L. carolinianum. Molecular gut content analysis provided evidence that B. cockerelli adults disperse between potato and Lycium. These results demonstrate that L. berlandieri and L. carolinianum are likely noncrop sources of potato-colonizing B. cockerelli in South Texas and that L. carolinianum is a potential source of Lso-infected psyllids. We also routinely collected the congeneric psyllid, Bactericera dorsalis (Crawford), from both Lycium species. These records are the first for this psyllid in Texas. Bactericera dorsalis completed development on both native Lycium species, albeit with high rates of mortality on L. berlandieri. B. dorsalis acquired and transmitted Lso on L. carolinianum under greenhouse conditions but did not transmit Lso to potato. These results document a previously unknown vector of Lso.
Primary resistance to integrase inhibitors in Shenzhen
Authors: Zhu Yue , Huang Yuanmei , Zheng Chenli , Tang Jie , Zeng Guang , Xie Wei , Wang Hui , Zhang Lukun , Liu Shaochu , Zhang Yan , Tan Wei , Tan Jingguang , Jiang Lijuan , He Yun , Xu Liumei , Yang Zhengrong , Zhao Jin 
Published: December 2022

Abstract

Objectives
In recent years, integrase strand transfer inhibitor (INSTI)-containing regimens have been increasingly adopted in treatment for HIV/AIDS and promoted as non-occupational post-exposure prophylaxis in China. This study aims to describe the prevalence of resistance to integrase and drug resistance mutations (DRMs) among ART-naive patients in Shenzhen, China.

Methods
Serum samples and demographic information were collected from newly reported ART-naive patients in Shenzhen in 2020. The study sequenced the coding sequence of the HIV-1 integrase gene and determined the DRMs.​

Results
In this study, 1682 newly reported cases were included and 1071 of them were successfully sequenced finally. The prevalence of primary drug resistance was 1.77%, with 19 samples showing varying degrees of resistance to INSTIs. The study detected six major DRMs in 16 individuals and eight accessory DRMs in 24 individuals. The prevalence of transmitted drug resistance (TDR) mutations was 1.21%, with five transmitted mutations detected in 13 individuals. The prevalence of drug resistance to raltegravir and elvitegravir was statistically higher than to bictegravir, cabotegravir and dolutegravir.

Conclusions
The prevalence of INSTI resistance in Shenzhen in 2020 was relatively high. ​Continued surveillance for resistance to INSTIs is recommended and treatment regimens should be adopted based on the pattern of resistance to INSTIs. ​Dolutegravir or bictegravir is first recommended when considering INSTIs as treatment regimens.
Understanding the relationship between weather variables and intake in beef steers
Authors: Yusuf Mustapha , Swanson Kendall C , Hulsman Hanna Lauren L , Bauer Marc L 
Published: December 2022

Abstract
The relationship between weather variables and dry matter intake (DMI) in beef steers was examined using daily intake data from 790 beef steers collected through a computer-controlled feeding system in nonsummer months. Daily data were condensed into weekly averages (N = 13,895 steer-weeks). The variables considered to predict DMI (2.50 to 23.60 kg/d) were body weight (197 to 796 kg), dietary net energy for maintenance (NEm; 0.79 to 2.97 Mcal/kg), ambient temperature (−23.73 °C to 21.40 °C), range of temperature (2.79 °C to 19.43 °C), dew point (−27.84 °C to 14.34 °C), wind speed (2.08 to 6.49 m/s), solar radiation (30.8 to 297.1 W/m2), and 2-wk lag (average of previous 2 wk’s values) and monthly lag (average of previous 4 wk’s values) of each weather variable. Toeplitz variance-covariance structure for repeated measures was used to determine the model to predict DMI, while accounting for the effects of body weight, dietary NEm, and other variables in the model. Two-week lag of ambient temperature interacted (P ≤ 0.005) with 2-wk lag of range of temperature, monthly lag of wind speed, 2-wk lag of solar radiation, and dew point to predict DMI. Interactions (P = 0.0001) between 2-wk lag of range of temperature vs. dew point and monthly lag of wind speed vs. 2-wk lag of solar radiation were also detected. This study reports important weather variables associated with differences in DMI of growing and finishing steers and will help improve the accuracy of DMI prediction equations for beef cattle. Improvements in the accuracy of predicting DMI should give producers better tools to plan and execute efficient feeding management programs. The R2 of the overall model was 0.8891.
Characterization of sow milk N-linked glycoproteome over the course of lactation
Authors: Rajput Prabha , Aryal Uma K , Bhide Ketaki , Minor Radiah C , Krishnamurthy Sairam , Casey Theresa M 
Published: December 2022

Abstract
Milk proteins serve as nutrition and affect neonate development and immunity through their bioactivity. Post-translational modifications of proteins affect their bioactivity. Glycosylation is the attachment of sugar moieties to proteins, with attachment of glycans to asparagine indicated as N-linked glycosylation. Our objective was to characterize N-linked glycosylated proteins in homogenate swine milk samples collected from sows (n = 5/6) during farrowing to represent colostrum and on days 3 and 14 post-farrowing to represent transitional and mature milk, respectively. Glycopeptides were isolated with lectin-based extraction and treated with Peptide N-glycosidase F (PNGase F) to identify N-linked glycosylation sites. Purified glycopeptides were analyzed by label-free liquid chromatography–tandem mass spectrometry (LC–MS/MS). MaxQuant software was used to align spectra to Sus scrofa Uniport database to identify proteins and measure their relative abundances. Analysis of variance and Welch’s t-test analysis identified glycoproteins differentially abundant between colostrum, transitional, and mature milk (false discovery rate &lt;0.05). Shotgun proteome analysis identified 545 N-linked and glutamine, Q, -linked, glycosylation (P &gt; 0.75 for deamidation) sites on 220 glycoproteins in sow milk. Glycoproteins were found across all three phases of swine milk production and varied by number of glycosylation sites (1–14) and in abundance and distribution between colostrum, transitional, and mature milk. Polymeric immunoglobulin receptor was the most glycosylated protein with 14 sites identified. Also highly glycosylated were casein and mucin proteins. These data are described and the relevance of glycosylated milk proteins in neonate development, such as protection against pathogens, is discussed.
The Japanese health care system-based cost-effectiveness of photodynamic diagnosis-assisted transurethral resection of bladder tumor for non-muscle invasive bladder cancer
Authors: Nishimura Nobutaka , Miyake Makito , Miyamoto Tatsuki , Tomizawa Mitsuru , Shimizu Takuto , Morizawa Yosuke , Hori Shunta , Gotoh Daisuke , Nakai Yasushi , Torimoto Kazumasa , Tanaka Nobumichi , Fujimoto Kiyohide 
Published: December 2022

AbstractBackgroundPhotodynamic diagnosis-assisted transurethral resection of bladder tumor reduces the risk of intravesical recurrence compared with conventional white light-transurethral resection of bladder tumor. However, the patient burden of costs for photodynamic diagnosis-transurethral resection of bladder tumor is higher than that for white light-transurethral resection of bladder tumor per installment, and the impact of the medical economics of photodynamic diagnosis-transurethral resection of bladder tumor is unclear. Therefore, we evaluated the Japanese health care system-based cost-effectiveness of photodynamic diagnosis-transurethral resection of bladder tumor compared with that of white light-transurethral resection of bladder tumor.MethodsWe conducted a retrospective chart review of 100 patients who underwent initial white light- or photodynamic diagnosis-transurethral resection of bladder tumor for non-muscle invasive bladder cancer from February 2012 to August 2019. Cumulative intravesical recurrences during 1000 post-operative days after the initial transurethral resection of bladder tumor were counted. Furthermore, the cumulative costs were calculated using the Diagnostic Procedure Combination and Per-Diem Payment System unique to Japan. The costs/year/person calculated using the person-year method was compared between the white light- and photodynamic diagnosis-transurethral resection of bladder tumor.ResultsAmong the 100 patients, 40 (40%) and 60 (60%) underwent the initial white light- and photodynamic diagnosis-transurethral resection of bladder tumor, respectively. The cumulative incidence of bladder recurrence requiring hospitalization and transurethral resection of bladder tumor was 20 and 5% for the white light-transurethral resection of bladder tumor and photodynamic diagnosis-transurethral resection of bladder tumor, respectively. The costs for hospitalization and surgical procedures per white light- or photodynamic diagnosis-transurethral resection of bladder tumor were 348 228 and 481 820 Japanese yen, respectively. The cost/year/person by the person-year method was 8073 and 8557 Japanese yen for the white light- and photodynamic diagnosis-transurethral resection of bladder tumor, respectively.ConclusionsThe cost/year/person for hospitalization and surgical procedures was slightly different between the white light- and photodynamic diagnosis-transurethral resection of bladder tumor. Furthermore, photodynamic diagnosis-transurethral resection of bladder tumor can reduce intravesical recurrence and is more cost-effective than white light-transurethral resection of bladder tumor.
A case of fibroadenoma with lobular carcinoma in situ and microinvasion in a young woman
Authors: Wang Mingjia , Leong May Ying , Tan Qing Ting 
Published: December 2022

Abstract
Fibroadenomas are benign breast tumors with a rare incidence of malignant transformation. Carcinoma arising within fibroadenomas typically occurs in women above the age of 40. We present a case of a young patient with microinvasive carcinoma arising within a biopsy-proven fibroadenoma, highlighting the possibility of carcinoma arising within fibroadenomas in young patients and the importance of vigilant surveillance even after a benign biopsy. In younger patients, new suspicious changes in benign lesions should be investigated. If prior core needle biopsy was performed, excision biopsy should be considered as the malignancy may be small and may be missed on needle biopsy.
The nuclear effector ArPEC25 from the necrotrophic fungus Ascochyta rabiei targets the chickpea transcription factor CaβLIM1a and negatively modulates lignin biosynthesis, increasing host susceptibility
Authors: Singh Shreenivas Kumar , Shree Ankita , Verma Sandhya , Singh Kunal , Kumar Kamal , Srivastava Vikas , Singh Ritu , Saxena Samiksha , Singh Agam Prasad , Pandey Ashutosh , Verma Praveen Kumar 
Published: December 2022

Abstract
Fungal pathogens deploy a barrage of secreted effectors to subvert host immunity, often by evading, disrupting, or altering key components of transcription, defense signaling, and metabolic pathways. However, the underlying mechanisms of effectors and their host targets are largely unexplored in necrotrophic fungal pathogens. Here, we describe the effector protein Ascochyta rabiei PEXEL-like Effector Candidate 25 (ArPEC25), which is secreted by the necrotroph A. rabiei, the causal agent of Ascochyta blight disease in chickpea (Cicer arietinum), and is indispensable for virulence. After entering host cells, ArPEC25 localizes to the nucleus and targets the host LIM transcription factor CaβLIM1a. CaβLIM1a is a transcriptional regulator of CaPAL1, which encodes phenylalanine ammonia lyase (PAL), the regulatory, gatekeeping enzyme of the phenylpropanoid pathway. ArPEC25 inhibits the transactivation of CaβLIM1a by interfering with its DNA-binding ability, resulting in negative regulation of the phenylpropanoid pathway and decreased levels of intermediates of lignin biosynthesis, thereby suppressing lignin production. Our findings illustrate the role of fungal effectors in enhancing virulence by targeting a key defense pathway that leads to the biosynthesis of various secondary metabolites and antifungal compounds. This study provides a template for the study of less explored necrotrophic effectors and their host target functions.
Elevated CO2 counteracts effects of water stress on woody rangeland-encroaching species
Authors: O’Connor Rory C , Blumenthal Dana M , Ocheltree Troy W , Nippert Jesse B 
Published: December 2022

AbstractThe ubiquity of woody plant expansion across many rangelands globally has led to the hypothesis that the global rise in atmospheric carbon dioxide concentration ([CO2]) is a global driver facilitating C3 woody plant expansion. Increasing [CO2] also influences precipitation patterns seasonally and across the landscape, which often results in the prevalence of drought in rangelands. To test the potential for [CO2] to facilitate woody plant growth, we conducted a greenhouse study for 150 days to measure CO2 effects on juveniles from four woody species (Cornus drummondii C.A. Mey., Rhus glabra L., Gleditsia triacanthos L., Juniperus osteosperma Torr.) that are actively expanding into rangelands of North America. We assessed chronic water-stress (nested within CO2 treatments) and its interaction with elevated [CO2] (800 p.p.m.) on plant growth physiology for 84 days. We measured leaf-level gas exchange, tissue-specific starch concentrations and biomass. We found that elevated [CO2] increased photosynthetic rates, intrinsic water-use efficiencies and leaf starch concentrations in all woody species but at different rates and concentrations. Elevated [CO2] increased leaf starch levels for C. drummondii, G. triacanthos, J. osteosperma and R. glabra by 90, 39, 68 and 41%, respectively. We also observed that elevated [CO2] ameliorated the physiological effects of chronic water stress for all our juvenile woody species within this study. Elevated [CO2] diminished the impact of water stress on the juvenile plants, potentially alleviating an abiotic limitation to woody plant establishment in rangelands, thus facilitating the expansion of woody plants in the future.
Mpox detection in the wastewater and the number of hospitalized patients in Poznan, Poland
Authors: Gazecka Monika , Sniezek Jakub , Maciolek Krzysztof , Kowala-Piaskowska Arleta , Zmora Pawel 
Published: December 2022

AbstractWe monitored for mpox in the Poznan wastewater system from July to December 2022 and compared its occurrence with the number of hospitalizations. Our results suggest that the scale of the epidemic is underestimated, and many mpox-infected individuals are not identified by the public health authority.Article summary lineWastewater-based epidemiology can determine the scale of an epidemic and estimate the number of infected individuals not under public health authorities.
COVID-19 in Cambodia, January 2020–June 2022: a success story
Authors: Chhim Srean , Ku Grace Marie , Mao Sovathiro , van de Put Willem , Van Damme Wim , Ir Por , Chhorvann Chhea , Or Vandine 
Published: December 2022

ABSTRACTAs a member state of the International Health Regulation 2005, Cambodia has been continuously strengthening its capacity to respond to health emergencies and prevent the international spread of diseases. Despite this, Cambodia’s capacity to prevent, detect, and rapidly respond to public health threats remained limited at the onset of the pandemic. This paper describes epidemiological phases, response phases, strategy, and lessons learned in Cambodia between 27 January 2020 and 30 June 2022. We classified epidemiological phases in Cambodia into three phases, in which Cambodia responded using eight measures:(1) detect, isolate/quarantine; (2) face coverings, hand hygiene, and physical distancing measures; (3) risk communication and community engagement; (4) school closures; (5) border closures; (6) public event and gathering cancellation; (7) vaccination;and(8) lockdown. The measures corresponded to six strategies:(1) setting up and managing a new response system, (2) containing the spread with early response, (3) strengthening the identification of cases and contacts, (4) strengthening care for COVID-19 patients, (5) boosting vaccination coverage, and(6) supporting disadvantaged groups. Finally, ten lessons were learned for future health emergency responses. Findings suggest that Cambodia successfully contained the spread of SARS-CoV-2 in the first year and quickly attained high vaccine coverage by the second year of the response. The core of this success was the strong political will and high level of cooperation from the public. However, Cambodia needs to further improve its infrastructure for quarantining and isolating cases and close contacts and laboratory capacity for future health emergencies.SUMMARY BOXCOVID-19 spread globally, but how the pandemic played out in each country depended on various factors, including government responses and the general public’s adherence to COVID-19 measures.Early response—Early detection, Early isolation, Early tracing, Early treatment, and Early education—is the core of successful SARS-CoV-2 containment.Achieving high vaccination coverage quickly leads to a decline in the number of deaths and to eventual full re-opening of the country.Responding to the pandemic requires decisive leadership and good governance, that refers to decisions being made quickly, in a timely manner, and without delay.High level of cooperation from the public is a fundamental factor for success in containing the spread in the early phase, and the massively successful vaccination campaign in the later stage.
Geographic distribution of conversion therapy prevalence in Canada
Authors: Tiwana Amrit , Salway Travis , Schillaci-Ventura Julia , Watt Sarah 
Published: December 2022

AbstractConversion therapy practices (CTPs) are discredited efforts that target lesbian, gay, bisexual, trans, queer, Two-Spirit, or other (LGBTQ2S+) people and seek to change, deny, or discourage their sexual orientation, gender identity, and/or gender expression. This study aims to investigate the prevalence of CTPs across Canadian provinces and territories and identify whether CTP bans are associated with decreasing prevalence. We analyzed 119 CTPs reported from 31 adults (18+) in Canada who have direct experience with CTPs, know people who have gone to CTPs, or know of conversion therapy practitioners by using a 2020 anonymous online survey. Mapping analysis was conducted using ArcGIS Online. CTP prevalence was compared between provinces/territories with and without bans using chi-square tests. We found 3 provinces and 11 municipalities had CTP bans. The prevalence of CTPs in provinces/territories with a ban is 2.34 per 1,000,000 population (95% CI 1.65, 3.31). The prevalence of CTPs in provinces/territories without a ban is 4.13 per 1,000,000 population (95% CI 3.32, 5.14). Accounting for the underlying population, provinces/territories with the highest prevalence of CTPs are New Brunswick (6.69), Nova Scotia (6.50), and Saskatchewan (6.37). Findings suggest only 55% of Canadians are protected under CTP bans. The prevalence of CTPs in provinces/territories without a ban is 1.76 times greater than in provinces/territories with a ban. CTPs are occurring in most provinces/territories, with higher prevalence in the west and the Atlantic. Findings will help inform policymakers and legislators as they are increasingly acknowledging CTPs as a threat to the health and well-being of LGBTQ2S+ people.
Molecular & Translational Biology of the Blood-Based VeriStrat® Proteomic Test Used in Cancer Immunotherapy Treatment Guidance
Authors: Koc Matthew A , Wiles Timothy Aaron , Weinhold Daniel C , Rightmyer Steven , Roder Joanna , Asmellash Senait , Roder Heinrich , Georgantas Robert W 
Published: December 2022

AbstractINTRODUCTIONThe blood-based VeriStrat®proteomic test (VS) predicts patient response to therapy based on the intensities of eight different features in a mass spectrum obtained from MALDI-TOF analysis of human serum/plasma specimens. An interim analysis of the INSIGHT clinical trial (NCT03289780) demonstrated that VS labels, VS Good and VS Poor, predict patients with non-small cell lung cancer (NSCLC) who are likely sensitive or resistant to immune checkpoint inhibitor (ICI) therapy [1]. While VS measures intensities of eight spectral features by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry from patient serum/plasma samples, the individual proteoforms underlying these features have not been rigorously and comprehensively identified.OBJECTIVESThe objective of this study was to identify the proteoforms measured by VS.METHODSMass spectra for VS are acquired using a standard low-resolution MALDI-TOF procedure that generates broad, composite features. DeepMALDI [2] analysis of serum samples was used to resolve these features into finer peaks. Top-down proteomics analysis of human serum, combining reversed-phase fractionation and liquid chromatography – tandem mass spectrometry (LC-MS/MS), was then used to identify the key proteoform constituents of these peaks.RESULTSIt was determined that proteoforms of serum amyloid A1, serum amyloid A2, serum amyloid A4, C-reactive protein, and beta-2 microglobulin are primary constituents of the VS spectral features.CONCLUSIONProteoforms of several proteins related to host immunity were identified as major constituents of these features. This information advances our understanding of how VS can predict patient response to therapy and opens the way for further translational studies.HighlightsThe combination of top-down proteomics and DeepMALDI®spectrometry enables the identification of proteoforms measured by the VeriStrat Proteomic test.Proteoforms of serum amyloid A1 (SAA1), SAA2, SAA4, beta-2 microglobulin, and C-reactive protein are the primary constituents of the spectral features measured in the VeriStrat proteomic test.The proteins assayed by the VeriStrat proteomic test have individual prognostic value for oncology and immuno-oncology outcomes.The proteins assessed by the VeriStrat proteomic test have been shown to have direct effects on patient immune activity.