bluuebunny/crossref_metadata_2025_split huggingface dataset:
The ligand interactions of B cell Siglecs are involved in the prevention of autoimmunity to sialylated self-antigens and in the quality control of signaling-competent B cells
Authors: Tsubata Takeshi 
Published: July 2023

Abstract
Sialic acid-binding immunoglobulin-like lectins (Siglecs) are a family of membrane molecules that recognize sialic acid. Most of them are inhibitory receptors that inhibit immune-cell activation by recognizing sialic acid as a self-motif. Human B cells express CD22 (also known as Siglec-2), Siglec-5, Siglec-6 and Siglec-10 whereas mouse B cells express CD22 and Siglec-G (ortholog of human Siglec-10). Siglecs recognize both sialylated molecules expressed on the same cell (cis-ligands) and those expressed by other cells (trans-ligands). In Guillain–Barré syndrome (GBS), antibody production to gangliosides (which are sialic acid-containing glycolipids) expressed by neurons plays a pathogenic role. A Siglec-10 variant deficient in recognition of gangliosides is genetically associated with GBS, suggesting that Siglec-10 induces self-tolerance to gangliosides by recognizing gangliosides as trans-ligands. Recognition of the BCR as a cis-ligand by Siglec-G and CD22 suppresses BCR signaling in B-1 cells and conventional B cells, respectively. This signal suppression prevents excess expansion of B-1 cells and is involved in the quality control of signaling-competent B cells by setting a threshold for tonic signaling during B cell development. CD22 recognizes other cis-ligands including CD22 and β7 integrin. Interaction of CD22 with other CD22 molecules induces CD22 clustering that suppresses CD22-mediated signal inhibition upon BCR ligation, and interaction with β7 integrin maintains its function in the gut-homing of B cells. Taken together, interactions of B cell Siglecs with multiple trans- and cis-ligands play important roles in B cell homeostasis and immune responses.
The c-di-GMP signalling component YfiR regulates multiple bacterial phenotypes and virulence in Pseudomonas plecoglossicida
Authors: Wang Ying , Jin Yinhua , Sun Fei , Zhang Yuanxing , Liu Qin , Wang Qiyao , Yang Dahai , Zhang Yibei 
Published: July 2023

Abstract

Aims
Pseudomonas plecoglossicida (P. plecoglossicida) is the causative agent of visceral granulomas disease in large yellow croaker (Larimichthys crocea) and it causes severe economic loss to its industry. Biofilm formation, related to intracellular cyclic bis (3′–5′) diguanylic acid (c-di-GMP) levels, is essential for the lifestyle of P. plecoglossicida. This research aims to investigate the role of YfiR—a key regulator of the diguanylate cyclase YfiN to regulate c-di-GMP levels and reveal its regulatory function of bacterial virulence expression in P. plecoglossicida.

Methods and results
A genetic analysis was carried out to identify the yfiBNR operon for c-di-GMP regulation in P. plecoglossicida. Then, we constructed a yfiR mutant and observed increased c-di-GMP levels, enhanced biofilm formation, increased exopolysaccharides, and diminished swimming and swarming motility in this strain. Moreover, through establishing a yolk sac microinjection infection model in zebrafish larvae, an attenuated phenotype of yfiR mutant that manifested as restored survival and lower bacterial colonization was found.

Conclusions
YfiR is the key regulator of virulence in P. plecoglossicida, which contributes to c-di-GMP level, biofilm formation, exopolysaccharides production, swimming, swarming motility, and bacterial colonization in zebrafish model.
Changes in antibacterial activity, colour, and hydrogen peroxide content of Western Australian Jarrah and Marri honeys after storage at different temperatures over time
Authors: Jones Zachary J M , Huang Yina , Green Kathryn J , Hammer Katherine A 
Published: July 2023

AbstractAimsThis study aimed to evaluate the effects of storage and different temperatures on the antibacterial activity and physicochemical characteristics of several types of honey.Methods and resultsHoneys stored for 16 weeks at 37 and 45°C showed significant declines in antibacterial activity determined by minimum inhibitory concentrations, the loss of hydrogen peroxide, decreases in honey pH, and increases in honey colour, with changes most pronounced at 45°C. In contrast, honeys stored for 16 weeks at ambient (∼22°C) and cold (4, −20, and −80°C) temperatures showed only minor changes. In a second set of 12 honeys stored for 16–32 months at ambient temperature and then 4°C, honeys showed minor changes in antibacterial activity, increases in colour, and decreases in pH. For a third set of 17 honeys stored for five years at ambient temperature, the honeys showed almost complete loss of hydrogen peroxide and were all significantly darker in colour, but showed varied changes in antibacterial activity.ConclusionsHeat was detrimental to the antibacterial activity of honeys, as was long-term storage at ambient temperatures for some honeys but not others.
Validation of the KangarooSci® Aerobic Count Plate for the Enumeration of Meosphilic Aerobic Bacteria in Selected Foods and on Stainless Steel Environmental Surfaces: AOAC Performance Tested MethodSM 062301
Authors: Wei Danping , Wu Di , Wang Jisheng , Li Yazhou , Sun Xinyu , Zhang Can , Zhang Shuai , Thompson Wesley , Benzinger Jr M Joseph , Crowley Erin 
Published: July 2023

Abstract

Background
The KangarooSci® Aerobic Count Plate (ACP) is a sample-ready culture medium system for direct counting of aerobic bacteria colonies after 48–72 h of incubation.

Objective
The KangarooSci ACP was evaluated for AOAC Performance Tested MethodsSM certification.

Methods
The KangarooSci ACP was evaluated through matrix studies and product consistency/stability study and robustness testing. For the matrix study, nine food products (nonfat dry milk powder, fresh raw bovine milk, pasteurized liquid bovine milk, fresh raw ground beef, frozen uncooked chicken breast, cooked shredded pork, apple juice, ice cream, and fresh strawberries), and one environmental surface (stainless steel) were evaluated following the KangarooSci ACP product instructions and compared to the ISO 4833–1:2013, Microbiology of food and animal feeding stuffs—Horizontal methods for the enumeration of microorganisms—Part 1: Colony count at 30 °C by the pour plate technique reference standard. The product consistency and stability testing evaluated three separate production lots of the KangarooSci ACP. The robustness testing examined three test parameters, volume of sample plated, incubation time, and incubation temperature, using a factorial study design.

Results
Results from the matrix study demonstrated equivalent performance between the KangarooSci ACP and the ISO 4833–1:2013 reference standard. The product consistency and stability testing showed that the performance of the assay was equivalent over time up to 12 months and between production lots. Minor changes to the operational test conditions showed no significant impact on performance during the robustness testing.

Conclusion
The KangarooSci ACP is an effective method for aerobic plate count for all matrixes evaluated.

Highlights
The KangarooSci ACP allows for fast, reliable enumeration of aerobic bacteria. Utilizing the alternative method takes up less space in incubators, requires no sample spreader, and requires fewer consumables compared to the reference method.
Water alternative gas (WAG) optimization for a heterogeneous Brazilian pre-salt carbonate reservoir
Authors: Ali Elbaloula Husham A , de Oliveira Gonçalo Soares , Schiozer Denis J 
Published: July 2023

Abstract
Water alternating gas (WAG) is a cyclical process that involves alternating water and gas injections with the primary goal to improve sweep efficiency by maintaining initial high pressure, slowing water and gas breakthrough, and lowering oil viscosity. The objective of this work is to apply and optimize a WAG strategy on a carbonate field with light oil, compare it to the initially planned water-flooding strategy, and investigate the capability of WAG to improve field production. In this research, a compositional reservoir simulator was used to model a WAG process by injecting produced gas into the reservoir, using the same well structure as an optimized water-flooding strategy. Subsequently, a WAG strategy was created, optimizing the number and locations of wells, to facilitate a comparative analysis of the two recovery methods. The WAG optimization involved a detailed assessment of variables such as bottom hole pressure (BHP), WAG cycle duration, maximum gas oil ratio (GOR), and well positioning, to achieve a high net present value (NPV). The study focuses on the application of WAG optimization modeling in unconventional reservoirs, specifically pre-salt carbonate reservoirs, and investigates its implications on production strategy and forecast, emphasizing its potential for maximizing NPV and oil recovery in a recently producing field. The results showed that WAG improved reservoir performance when compared to water injection and produced a greater amount of oil. This solution showed potential to be tested under uncertainties (reservoir heterogeneity, faults, fractures, karsts, vugs, etc.) as future steps.
Equity illusions
Authors: Aran Yifat , Murciano-Goroff Raviv 
Published: July 2023

Abstract
Although equity-compensation grants for rank-and-file employees are common in startups and are considered an ingrained part of their business culture, little is known about how employees approach this form of compensation. We begin filling this gap by examining employees’ financial literacy regarding equity compensation and their willingness to forgo cash compensation for startup equity. Using a survey and a combination of natural language processing and machine learning techniques with conventional regression modeling, we find that employees commonly respond to economically irrelevant signals and misinterpret other important signals. The findings suggest that employees harbor a range of “market illusions” regarding startup equity that can lead to inefficiencies in the labor market, which sophisticated employers can legally exploit. The results raise questions about the protection of employees in their investor capacity in a market where highly sophisticated repeat players, such as venture capital investors, interact with unorganized and uninformed retail investors.
Guided Imagery and Music in the Treatment for Breast and Gynecologic Cancer: An RCT Pilot
Authors: Papanikolaou Evangelia , McKinney Cathy , Hannibal Niels , Tsouvelas George , Panoskaltsis Theodore , Karageorgopoulou Sofia 
Published: July 2023

Abstract
The primary aim of this randomized controlled trial pilot was to investigate the potential effectiveness of the music psychotherapy method, Guided Imagery and Music (GIM), to assist female patients who are undergoing chemotherapy treatment for breast or gynecologic cancer. A secondary aim was to suggest performance improvements regarding the protocol and the resources required to undertake a future larger scale study. The researchers randomized participants (n = 20) into two groups: Intervention group (n = 10) and Control group (n = 10). The Intervention group received a series of six individual, short GIM sessions whereas the Control group received two verbal counseling sessions that took place at Week 1 and at Week 6 of treatment. All participants in the study completed the Profile of Mood States (POMS-Brief); Cancer Fatigue Scale (CFS); and Herth Hope Index (HHI) before the beginning of Week 1 and again after Week 6 or, in the case of the Intervention group, after the last GIM session. All participants also completed two Visual Analogue Scales (VAS-Hope; VAS-Fatigue) weekly or after each session throughout the duration of the trial. The results of the Intervention group receiving GIM showed medium pre–post effect sizes for the CFS, HHI, and POMS questionnaires, and significant positive changes for the VAS-H and VAS-F questionnaires. As seen from this initial data outcome, a brief series of GIM sessions shows promise to be beneficial for increasing hope, decreasing fatigue, and mitigating distressed mood for female patients undergoing treatment for breast or gynecologic cancer.
Adverse Birth Experiences and Parent Adjustment Associated With Atypical Genital Appearance Due to Differences of Sex Development
Authors: Traino Katherine A , Fisher Rachel S , Basile Nathan L , Dattilo Taylor M , Baskin Laurence S , Buchanan Cindy L , Chan Yee-Ming , Cheng Earl Y , Coplen Douglas E , Kolon Thomas F , Lakshmanan Yegappan , Palmer Blake W , Mullins Larry L , Ciciolla Lucia M , Wisniewski Amy B 
Published: July 2023

Abstract

Objective
Differences/disorders of sex development (DSDs) are rare, congenital conditions involving discordance between chromosomes, gonads, and phenotypic sex and are often diagnosed in infancy. A key subset of parents of children newly diagnosed with a DSD experience clinically elevated distress. The present study examines the relationship between perinatal factors (i.e., gestational age, delivery method) and trajectories of parental adjustment.

Methods
Parent participants (mothers = 37; fathers = 27) completed measures at baseline, 6- and 12-month follow-up. Multilevel linear regression controlled for clustering of the data at three levels (i.e., time point, parent, and family) and examined the relationship between perinatal factors and trajectories of depressive and anxious symptoms. Two-way interactions between perinatal factors and parent type were evaluated.

Results
Overall depressive and anxious symptoms decreased over time. There were significant interactions between gestational age and parent type for depressive and anxious symptoms, with younger gestational age having a stronger negative effect on mothers vs. fathers. There was a significant interaction between time and gestational age for depressive symptoms, with 36 weeks’ gestational age demonstrating a higher overall trajectory of depressive symptoms across time compared to 38 and 40 weeks. Findings for the delivery method were not significant.

Conclusions
Findings uniquely demonstrated younger gestational age was associated with increased depressive symptoms, particularly for mothers compared to fathers. Thus, a more premature birth may predispose parents of infants with DSD to distress. Psychosocial providers should contextualize early diagnosis-related discussions within stressful birth experiences when providing support.
Pain-Related Stigma and Its Associations With Clinical and Experimental Pain Severity in Youth With Chronic Musculoskeletal Pain Conditions
Authors: Boileau Nicholas R , Thompson-Phillips Kathryn A , Goodin Burel R , Lynch-Milder Mary K , Evans Corinne T , Adetayo Tolulope , Rudolph Arionna F , Stoll Matthew L , Weiser Peter , Fobian Aaron D , Gowey Marissa A , Wakefield Emily O 
Published: July 2023

Abstract

Objective
Many children with chronic musculoskeletal pain conditions experience stigma which can have negative downstream consequences. This study compares ratings of clinical pain (current pain intensity and pain interference), experimental pain (temporal summation, cold water tolerance, and cold pain intensity), and pain-related stigma among three groups of youth with rheumatic conditions. The relations among ratings of pain-related stigma and pain variables were explored.

Methods
Eighty-eight youth aged 8–17 years with a diagnosis of juvenile idiopathic arthritis (JIA = 32), juvenile fibromyalgia (JFM = 31), or non-specific chronic pain (NSCP = 25) completed measures of clinical pain ratings (average 7-day pain intensity, day of assessment pain (DoA), and pain interference), experimental pain (cold pain tolerance, cold pain intensity, and temporal summation of mechanical pain), and pain-related stigma. Data analysis compared pain-related stigma and pain ratings across the three groups and examined the relations among pain-related stigma and pain ratings.

Results
Youth with JFM reported higher ratings of clinical pain and pain-related stigma than their counterparts with NSCP or JIA. However, there were no differences in experimental pain. Pain-related stigma was associated with greater ratings of pain interference, particularly for those with JIA and NSCP. Pain-related stigma was also associated with greater average daily pain intensity but not DoA.

Conclusion
Youth with medically unexplained pain report greater stigma and worse pain than their peers; thus, robust assessment of pain in this population is necessary. Future work should longitudinally explore the impact of pain-related stigma on pain outcomes and treatment responses.
Quantitative multiplex polymerase chain reaction in copies ml–1 linearly correlates with standard urine culture in colonies ml−1 for urinary tract infection (UTI) pathogens
Authors: Festa Richard A , Opel Mike , Mathur Mohit , Luke Natalie , Parnell Laura K S , Wang Dakun , Zhao Xinhua , Magallon Jesus , Percaccio Michael , Baunoch David 
Published: July 2023

Abstract
Standard urine culture (SUC) is the current standard method for confirmation of a urinary tract infection (UTI). SUC identifies microorganisms in urine samples and semi-quantifies these as colony-forming units (CFUs) ml−1. In contrast, quantitative multiplex polymerase chain reaction (q-MPCR) is a culture-independent assay in which the microbes are quantified by targeting genomic sequences and reported as cells ml−1, calculated from copies ml−1. Using serial dilutions within the 104–105 cells ml−1 range, the usual reporting range of SUC, this study compared the quantification results based on SUC and q-MPCR for four uropathogens with the control hemocytometer counts. The results revealed a linear relationship and a 1:1 correlation between the q-MPCR and SUC results. Additional q-MPCR quantification of 36 uropathogenic non-fastidious and fastidious bacteria and yeast indicated a reproducible linear correlation in a 1:1 manner with the control counts over a range of cell densities (103–106 cells ml−1). The results confirm that the quantifications by q-MPCR in cells ml−1 and by SUC in CFUs ml−1 are comparable and answer to the lingering question of how the results of these two methods correlate. Moreover, q-MPCR provided accurate quantification of various microorganisms over wider cell density ranges without the time required for microbial growth.
Densities and mass assembly histories of the Milky Way satellites are not a challenge to ΛCDM
Authors: Kravtsov Andrey , Wu Zewei 
Published: July 2023

ABSTRACT
We use the GRUMPY galaxy formation model based on a suite of zoom-in, high-resolution, dissipationless Λ Cold Dark Matter (ΛCDM) simulations of the Milky Way (MW) sized haloes to examine total matter density within the half-mass radius of stellar distribution, ρtot(&lt; r1/2), of satellite dwarf galaxies around the MW hosts and their mass assembly histories. We compare model results to ρtot(&lt; r1/2) estimates for observed dwarf satellites of the Milky Way spanning their entire luminosity range. We show that observed MW dwarf satellites exhibit a trend of decreasing total matter density within a half-mass radius, ρtot(&lt; r1/2), with increasing stellar mass. This trend is in general agreement with the trend predicted by the model. None of the observed satellites are overly dense compared to the results of our ΛCDM-based model. We also show that although the halo mass of many satellite galaxies is comparable to the halo mass of the MW progenitor at z ≳ 10, at these early epochs halos that survive as satellites to z = 0 are located many virial radii away from the MW progenitors and thus do not have a chance to merge with it. Our results show that neither the densities estimated in observed Milky Way satellites nor their mass assembly histories pose a challenge to the ΛCDM model. In fact, the broad agreement between density trends with the stellar mass of the observed and model galaxies can be considered as yet another success of the model.
The radio emission in radio-quiet quasars: the VLBA perspective
Authors: Chen Sina , Laor Ari , Behar Ehud , Baldi Ranieri D , Gelfand Joseph D 
Published: July 2023

ABSTRACT
The origin of the radio emission in radio-quiet quasars (RQQ) is not established yet. We present new VLBA observations at 1.6 and 4.9 GHz of 10 RQQ (9 detected), which together with published earlier observations of 8 RQQ (5 detected), forms a representative sample of 18 RQQ drawn from the Palomar–Green sample of low z (&lt; 0.5) AGN. The spectral slope of the integrated emission extends from very steep (α &lt; −1.98) to strongly inverted (α = +2.18), and the slopes of 9 of the 14 objects are flat (α &gt; −0.5). Most objects have an unresolved flat-spectrum core, which coincides with the optical Gaia position. The extended emission is generally steep-spectrum, has a low brightness temperature (&lt; 107 K), and is displaced from the optical core (the Gaia position) by ∼ 5–100 pc. The VLBA core flux is tightly correlated with the X-ray flux, and follows a radio to X-ray luminosity relation of log LR/LX ≃ −6, for all objects with a black hole mass log MBH/M⊙ &lt; 8.5. The flatness of the core emission implies a compact source size (≲ 0.1 pc), which likely originates from the accretion disc corona. The mas-scale extended emission is optically thin and of clumpy structure, and is likely produced by an outflow from the center. Radio observations at higher frequencies can further test the accretion disc coronal emission interpretation for the core emission in RQQ.
Cold diffuse interstellar medium of Magellanic Clouds: I. HD molecule and cosmic-ray ionization rate
Authors: Kosenko D N , Balashev S A 
Published: July 2023

ABSTRACT
HD molecule is one of the most abundant molecule in the Universe and due to its sensibility to the conditions in the medium, it can be used to constrain physical parameters in the medium where HD resides. Lately, we have shown that HD abundance can be enhanced in the low-metallicity medium. Large and Small Magellanic Clouds give us an opportunity to study low-metallicity galaxies in detail towards different sightlines due to their proximity to our Galaxy. We revisited Far Ultraviolet Spectroscopic Explorer (FUSE) space telescope archival spectra towards bright stars in Magellanic Clouds to search for HD molecules, associated with the medium of these galaxies. We reanalysed H2 absorption lines and constrained HD column density at the positions of H2 components. We detected HD towards 24 sightlines (including 19 new detections). We try to measure cosmic ray ionization rate for several systems using measured N(HD)/N(H2), and in most cases get loose constraints due to insufficient quality of the FUSE spectra.
Galactic properties that favour star cluster formation: a statistical view
Authors: Berek Samantha C , Reina-Campos Marta , Eadie Gwendolyn , Sills Alison 
Published: July 2023

ABSTRACT
The presence or absence of star clusters in galaxies, and the properties of star cluster populations compared to their host galaxy properties, are important observables for validating models of cluster formation, galaxy formation, and galaxy assembly. In this work, we apply a Bayesian approach to fit two models to data from surveys of young clusters in star-forming galaxies. The first model is a logistic regression, which allows us to include galaxies which do not have any young clusters. The second model is a hurdle model, which includes galaxies with zero clusters and also incorporates information about the total mass in the cluster system. We investigate two predictors (star formation rate and total stellar mass in the galaxy) and look at clusters younger than 10 or 100 Myr. We find that in all cases, star formation rate is the better predictor for both the probability of hosting clusters and the total mass in the cluster system. We compare our results to similar models for old globular clusters, and conclude that star cluster formation was more abundant and more efficient at higher redshifts, likely because of the high gas content of galaxies at that time.