bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dosimetric Comparison between CyberKnife and HyperArc Treatment Plans for Trigeminal Neuralgia: Cone-Based Robotic System versus High- Definition MLC-Based Linac System
Authors: Yang Ching-Chieh , Ho Hsiu-Wen , Lin Hsiu-Man , Ting Wei-Chen , Wang Shih-Chang , Chen Hsiao-Yun , Lin Yu-Wei 
Published: August 2023

Abstract
Background
This study evaluates the feasibility of HyperArc (HA) for trigeminal neuralgia (TN) by comparing dose distribution with CyberKnife (CK).
Methods
Contour sets from twenty patients who had undergone CK for TN were used to generate HA treatment plans for comparison. Two different TN target delineation settings were used: the whole segment of the trigeminal nerve root entry zone (REZ) group and the 5-mm spherical target group. The prescribed dose was 65 Gy in a single fraction, prescribed to the 80% isodose line. The CK and HA treatment plans were compared for target coverage, sparing of organs at risk (OARs), and dose distribution metrics.
Results
In the whole segment of the REZ group, the HA plans showed statistically significant differences with higher target coverage than the CK plans. The mean brain doses for HA and CK were 0.83 Gy and 1.15 Gy, respectively (P < 0.001). The brain V12 was significantly smaller for HA plans (5.9 cm3) than CK plans (6.9 cm3). Significant achievement in the doses of the ipsilateral/contralateral cranial nerve (CN) VII/VIII were observed in the HA plans than the CK plans. The conformity index was significantly greater in the HA plans compared to the CK plans. The dose gradient radius was similar for the CK and HA plans. In the 5mm-spherical target group, both plans showed good target coverage, with the CK plans exhibiting better brain sparing and higher CN VII/VIII dose. The dose distribution metrics were similar for both plans.
Conclusions
The HA technique is a feasible alternative for TN treatment, offering excellent organ-at-risk sparing and favorable dosimetric distribution.
Prevalence of gastrointestinal helminth parasite infections in country chicken from Visakhapatnam district Andhra Pradesh India
Authors: Mathews Anisha , Pusupuleti Janikiram , Shameem Ummey 
Published: August 2023

Abstract
Birds in a free environment carry huge risk of helminth parasite infections affecting the health of poultry and thereby indirectly leading to great economic loss. The present study aimed to evaluate the prevalence, parasite intensity and species diversity of gastrointestinal helminth parasites (GIH) of country chicken (Gallus gallus domesticus) from local chicken markets of Visakhapatnam district, Andhra Pradesh. A total of 300 gastrointestinal tract (GIT) samples were examined from January to December 2021 and the collected parasites were separated, counted, identified and statistically analysed. The overall prevalence of infection was found to be 87% with an intensity of 139.29. The study showed high prevalence with mixed infections (66%), whereas the prevalence with individual groups i.e. cestodes, nematodes and trematodes was found to be 83.00%, 70.33%, and 0.67% respectively. Altogether 10 parasite species were identified viz. Raillietina echinobothrida being the most dominant with a prevalence of 72.67%, followed by Raillietina tetragona (62.00%), Heterakis gallinarum (59.33%), Ascaridia galli (45.33%), Davainea proglottina (43.00%), Amoebotaenia cuneata (42.67%), Raillietina cesticillus (38.33%), Hymenolepis sps. 1 (36.00%), Cotugnia sps. (29.67%) and the least was Hymenolepis sps. 2 (21.00%). In month-wise data, highest prevalence was recorded in July (100%), and the highest seasonal prevalence was recorded in summer (90%), followed by monsoon (87%) and winter (84%). No major significant difference in prevalence was noted between males (88.51%) and females (85.53%). Hosts with high body temperature and low GIT pH levels showed increased prevalence and parasite intensity. The present study helps in laying better health and disease management strategies for country chicken.
Chlorogenic acid attenuates deoxynivalenol-induced apoptosis and pyroptosis in human keratinocytes via activating Nrf2/HO-1 and inhibiting MAPK/NF-κB/NLRP3 pathways
Authors: Chen Jiashe , Zhou Zhiyu , Xi Ningyuan , Xu Mingyuan , Wu Nanhui , Wu Fei , Fu Qiaoting , Yan Guorong , Liu Yeqiang , Xu Xiaoxiang 
Published: August 2023

Abstract
Background and aim
Deoxynivalenol (DON) is a common mycotoxic contaminant, frequently found in food and feed, causing a severe threat to human and animal health. Because of the widespread contamination of DON, humans involved in agricultural practices may be directly exposed to DON through skin route. Chlorogenic acid (CGA) is a phenolic acid, which has anti-inflammatory and antioxidant properties. However, it is still unclear whether CGA can protect against DON-induced skin damage. Here, the effect of CGA on mitigating damage to human keratinocytes (HaCaT) triggered by DON, as well as its underlying mechanisms were investigated.
Methods
HaCaT cells were pre-incubated with CGA for 2 h, and then exposed with 0.25 µg/mL DON for 24 h, and cell viability was examined with CCK-8 assay. ROS (reactive oxygen species) was measured by flow cytometry mitochondrial and reactive oxygen species (mtROS) was measured fluorescence microscope. Superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) were measured by using corresponding kits.Proteins expression levels were determined by Western blot analysis.
Results
CGA pretreatment for 2 h significantly increased cell viability and reversed DON-induced oxidative stress by improving antioxidant enzyme activities such as SOD, GSH, CAT, reducing mtROS generation and enhancing mitochondrial function through activating Nrf2/HO-1 pathway. Moreover, CGA significantly increased the Bcl-2 protein expression, and decreased the protein expressions of Bax and cleaved caspased-3, and suppressed the phosphorylated of ERK, JNK, NF-κB. In addition, CGA could also inhibited the pyroptosis-related protein expressions including NLRP3, cleaved caspased-1 and cleaved IL-1β.
Conclusion
Our results suggest that CGA could attenuate DON-induced oxidative stress, inflammation and apoptosis through activating Nrf2/HO-1 pathway and inhibiting MAPK/NF-κB and pyroptosis signaling pathway.
Links among MRI features in paraspinal muscles, inflammatory processes, and related back pain in patients with lumbar disc herniation
Authors: Chen Xiaolong , Cui Peng , Li Yongjin , Wang Yu , Lu Shibao 
Published: August 2023

Abstract
Background
Recent studies have provided evidence that structural changes in paraspinal muscles are associated with intervertebral disc degeneration (IDD), ubiquitous with low back pain (LBP), and potentially thought to be regulated by inflammatory processes. However, the cascade links remain unclear.
Objective
The aims of this study were to investigate structural changes in paraspinal muscles that differed in healthy and lumbar disc herniation (LDH) patients, and LDH patients with and without LBP, and to determine the cascade link with the expression of inflammatory marker(s)
Methods
Cross-sectional areas (CSAs) and fatty degeneration of muscles were measured in this cascade prospective cohort study. Multifidus muscle (MM) tissue was procured from included individuals undergoing surgery. Gene expression was quantified using qPCR assays. Independent t-test, Chi-square, and Spearman correlation were used for evaluating the links among structural changes, expression of inflammatory markers, and clinical outcomes.
Results
Functional CSA and fatty degeneration of MM were larger in healthy group than LDH group. A significant increase in fat infiltration in MM in LBP group than in non-LBP group. TNF was 28-fold greater in high-fat infiltration group than low-fat infiltration group within MM. Expression of TNF and IL-1β in MM was moderately correlated with functional CSA and fatty degeneration of MM, which was moderately correlated with clinical outcomes.
Conclusions
Results support the hypothesis that IDD is associated with dysregulation of inflammatory state of local MM, which provides initial evidence that inflammatory dysregulation in paraspinal muscles has the potential for a broad impact on tissue health and LBP symptoms.
Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in microbiome samples
Authors: Buetas Elena , López Marta Jordán , Roldán Andrés López , D'Auria Giuseppe , Martínez-Priego Llucia , De Marco Griselda , Mira Alex , Carda-Diéguez Miguel 
Published: August 2023

Abstract
Background.
Sequencing variable regions of the 16S rRNA gene (≃300bp) with Illumina technology is commonly used to study the composition of human microbiota. Unfortunately, short reads are unable to differentiate between highly similar species. Considering that species from the same genus can be associated with health or disease it is important to identify them at the lowest possible taxonomic rank. Third-generation sequencing platforms such as PacBio SMRT, increase read lengths allowing to sequence the whole gene with the maximum taxonomic resolution. Despite its potential, full length 16S rRNA gene sequencing is not widely used yet. The aim of the current study was to compare the sequencing output and taxonomic annotation performance of the two approaches (Illumina short read sequencing and PacBio long read sequencing of 16S rRNA gene) in different human microbiome samples.
Results.
DNA from saliva, oral biofilms and faeces of 9 volunteers was isolated. Regions V3-V4 and V1-V9 were amplified and sequenced by Illumina Miseq and by PacBio Sequel II sequencers, respectively. With both platforms, a similar percentage of reads was assigned to the genus level (94.79% and 95.06% respectively) but with PacBio a higher proportion of reads were further assigned to the species level (55.23% vs 74.14%). Regarding overall bacterial composition, samples clustered by niche and not by sequencing platform. In addition, all genera with > 0.1% abundance were detected in both platforms for all types of samples. Although some genera such as Streptococcus tended to be observed at higher frequency in PacBio than in Illumina (20.14% vs 14.12% in saliva, 10.63% vs 6.59% in biofilm samples) none of the differences were statistically significant when correcting for multiple testing.
Conclusions.
The results presented in the current manuscript suggest that samples sequenced using Illumina and PacBio are mostly comparable. Considering that PacBio reads were assigned at the species level with higher accuracy than Illumina, our data support the use of PacBio technology for future microbiome studies, although a higher cost is currently required to obtain an equivalent number of reads per sample.
The potential of cricMAN1A2 as a novel biomarker for gout diagnosis
Authors: Guo Wenyan , Liu Kaixi , Sun Jianhong , zhang Qiaoxin , Du Mudan , Chen Yanrong , lin Xiaozhe , Wu Qingyu , Yang Xinran 
Published: August 2023

Abstract
Background
Gout is the most prevalent inflammatory arthritis, its gold standard of diagnosis is detection of monosodium urate crystals in joints. However, the invasive test limited its use in the diagnosis of gout. Thus, there is an urgent need to exploit a novel biomarker to predict and early diagnose the gout flare.
Methods
In this study, we aimed to screen out the potential biomarkers of gout from GEO database (GSE178825) through bioinformatics analysis.
Results
The results showed that 4994 DEGs (43 up-regulated genes and 13 down-regulated genes) were identified between gout patients and healthy control. DEGs were mostly enriched in DNA repair, sphingolipid biosynthetic process, membrane. MAN1A2 was the most important hub genes in the PPI network.And then a series of enrichment bioinformatics methods were performed, cricMAN1A2 was selected as novel biomarker, which levels was measured in 30 gout patients, 30 hyperuricemia patients and 30 healthy controls by qRT-PCR. Subsequently, ROC (receiver operating characteristic cuver) were used to evaluated the potential role of cricMAN1A2 as biomarker for gout. The levels of circMAN1A2 was significantly lower in the gout patients than those in healthy controls, with higher diagnostic efficiency(AUC(area under the ROC curve) = 0.86).
Conclusions
Our results provide key cricRNAs related to gout, and cricMAN1A2 could be a novel serum biomarker for gout diagnosis.
CRTAM promotes antitumor immune response in triple negative breast cancer by enhancing CD8+ T cell infiltration
Authors: Zheng Shuyue , Yang Benlong , Li Lun , Chen Ming , Zhang Liyi , Chi Weiru , Jiang Yizhou , Shao Zhi-Ming , Xiu Bingqiu , Chi Yayun , Wu Jiong 
Published: August 2023

Abstract
Background
Immunomodulatory (IM) subtype triple negative breast cancer (TNBC) has high immune cell signaling, cytokine signaling gene expression, and is more responsive to immunotherapy. The mechanism responsible for this phenomenon is not clear, but the gene, the cytotoxic and regulatory T cell molecule (CRTAM), seems to be involved in this process.
Methods
A total of 360 TNBC patients diagnosed with TNBC without any treatment before surgery at Fudan University Shanghai Cancer Center (FUSCC) was analyzed and their primary tumor tissue subjected to RNA sequencing. Combined with three RNA-seq datasets obtained from GEO database, LASSO regression analysis was used to screen for IM type TNBC specific genes. Survival analysis were made by Kaplan-Meier method. GO, KEGG and GSEA analysis were used to explored the effect of CRTAM on the tumor immune response. RT-qPCR and western blot were used to assay the IFN response. Immunocompetent mice were injected in situ with mice triple negative breast cancer cells stably overexpressing CRTAM. Flow cytometry and IHC were used to determine tumor infiltrated immune cells of mice tumor model.
Results
In this study, we found that CRTAM expression was elevated in IM type TNBC and predicted favorable overall survival and recurrence-free survival in FUSCC and TCGA TNBC patients cohorts. GO and KEGG analysis suggested that CRTAM was highly associated with immune responses and immune system processes. Notably, CRTAM overexpression induced STAT1 phosphorylation and increased the expression of interferon-stimulated genes. We also found that CRTAM enhanced tumor-associated immune cell infiltration, especially CD8+ T cells, which may be related to the increased expression of MHC class I molecules caused by CRTAM overexpression. And it suggested that CRTAM had the potential to predict the efficacy of immunotherapy in TNBC.
Conclusions
In summary, these results suggest that CRTAM plays an important role in enhancing the immune inflammatory response and promoting CD8 + T cell infiltration in TNBC, which has the potential to be a new biomarker for predicting patients’ immune checkpoint inhibitor responses.
Increasing in three units the order of convergence of iterative methods for solving nonlinear systems
Authors: Cordero Alicia , Leonardo-Sepúlveda Miguel A. , Torregrosa Juan R. , Vassileva María P. 
Published: August 2023

Abstract

This manuscript presents an innovative technique for high-precision iterative methods in nonlinear systems. The goal is to enhance the convergence order by three units with just one additional functional evaluation per iteration, while keeping the computational cost low. This new approach is incorporated into existing methods of order p, allowing for more accurate and efficient results. Numerical tests support the theoretical validity of the technique, highlighting its superiority over conventional p-order methods and its potential for solving complex problems. In summary, the manuscript introduces a promising technique that combines high precision and efficiency in approximating solutions for nonlinear systems, increasing the convergence order of iterative methods from p to p+3.
WITHDRAWN: Simultaneous adsorption of malachite green, methyl orange, and rhodamine B with TiO 2 /macadamia nutshells-derived activated carbon composite
Authors: Thuy Le Thi Thanh , Thuy Pham Thi , Anh Pham Thi Giang , Tien Nguyen Anh , Bich Nguyen Thi Hong , Khieu Dinh Quang 
Published: August 2023

Abstract
In this study, TiO2/activated carbon (TiO2/MAC) composite was synthesized from activated carbon prepared from macadamia nutshells and a water-soluble titanium complex, and it was used to simultaneously adsorb malachite green (MG), methyl orange (MO), and rhodamine B (RhB) from aqueous solutions. The kinetic studies show that the adsorption experimental data are best described by the pseudo-second-order kinetic model. The equilibrium data of the trinary-component system were analysed via the models that combine the three single-component isotherms (Langmuir, Freundlich, Sips) into the ideal adsorption solution theory (IAST) and the Langmuir and P-factor-Langmuir extended models. The results obtained from the AICs (Akaike Information Criterion) indicate that IAST incorporating the Langmuir model is the most suitable to describe the removal of the dyes in the trinary component solution. The high maximum adsorption capacities, qe, in the single/trinary solution for MG, RhB, and MO are 0.318/0.241, 0.212/0.314, and 0.291/0.145 mM·g–1, respectively. The thermodynamic analysis reveals that the adsorption is spontaneous and endothermic. The results of material regeneration through photocatalytic self-cleaning show that TiO2/MAC can be utilized as a sustainable alternative for the simultaneous elimination of textile dyes from effluents.
Phase 3 Trial of Recombinant Human Alkaline Phosphatase for Patients with Sepsis-Associated Acute Kidney Injury (REVIVAL)
Authors: Pickkers Peter , Angus Derek , Bass Kristie , Bellomo Rinaldo , Berg Erik van den , Bernholz Juliane , Bestle Morten H , Doi Kent , Doig Christopher , Ferrer Ricard , Francois Bruno , Gammelager Henrik , Goettrup Ulf , Hoste Eric , Iversen Susanne , Joannidis Michael , Kellum John , Liu Kathleen , Meersch Melanie , Mehta Ravindra , Millington Scott , Murray Patrick , Nichol Alistair , Ostermann Marlies , Pettila Ville , Solling Christopher , Winkel Matthias , Young Paul , Zarbock Alexander 
Published: August 2023

Abstract
Purpose: Ilofotase alfa is a human recombinant alkaline phosphatase with reno-protective effects that showed improved survival and reduced MAKE90 in sepsis-associated acute kidney injury (SA-AKI) patients. ‘REVIVAL’, was aphase 3 trial, conducted to confirm its efficacy and safety.
Methods: In this international double-blinded randomized-controlled trial, SA-AKI patients were enrolled <72 hours on vasopressor and <24 hours of AKI. The primary endpoint was 28-day all-cause mortality. The key secondary endpoint was Major Adverse Kidney Events up to day 90 (MAKE90).
Results: 650 patients were treated and analyzed for safety; and 649 for efficacy data (ilofotase alfa n=330; placebo n=319). The observed mortality rates in the ilofotase alfa and placebo groups were 27.9% and 27.9% (nominal one-sided p-value of 0.50) at 28 days, and 33.9% and 34.8% (p=0.41) at 90 days. The trial was stopped for futility on the primary endpoint. The observed proportion of patients with MAKE90 was 56.7% in the ilofotase alfa group vs. 64.6% in the placebo group (p=0.02), mainly due to the number of patients who received renal replacement therapy (28.2% vs. 36.4%). There was evidence of heterogeneity of treatment effect with a marked reduction in MAKE90 events in patients with pre-existent impaired renal function randomized to ilofotase alfa (p=0.024). Adverse events were reported in 67.9% and 75.0% patients in the ilofotase and placebo group.
Conclusion: Among critically ill patients with SA-AKI, ilofotase alfa did not improve day 28 survival. There may however be reno-protective properties, especially among patients with pre-existing renal disease. No safety concerns were identified.
Trial registration and date of registration: ClinicalTrials.gov number NCT04411472, May-28-2020
Analysing the contribution of intermittent rivers to beta diversity can improve freshwater conservation in Mediterranean rivers
Authors: Soria Maria , Cid Núria , Ortega Jean , Bini Luis Mauricio , Acosta Raúl , Gutiérrez-Cánovas Cayetano , Rodríguez-Lozano Pablo , Fortuño Pau , Vinyoles Dolors , Gallart Francesc , Prat Narcís , Bonada Núria 
Published: August 2023

Abstract

In Mediterranean climate regions, intermittent rivers (IRs) harbor highly dynamic communities with species and trait composition changing over time and space. Considering simultaneously multiple biodiversity facets and a spatiotemporal perspective is, therefore, key to develop effective conservation strategies for these ecosystems. We studied the spatiotemporal dynamics of aquatic macroinvertebrates in rivers of the western Mediterranean Basin by analysing (1) the taxonomic and functional richness and the local contribution to beta diversity (LCBD; measured considering taxonomic and functional facets) of perennial rivers and IRs over five sampling times, and (2) their relation with flow intermittence, local environmental uniqueness, and the number of anthropogenic impacts. Both analyses were also conducted for the subset of data including only IRs to compare values between their flowing and disconnected pool phases. According to our results, taxonomic and functional richness tended to be higher in perennial rivers than in IRs, while IR sites made the greatest contribution to taxonomic and functional LCBD. When comparing among IRs sites over time, higher values of taxonomic and functional LCBD corresponded mostly to their disconnected pool phase. Flow intermittence, the number of impacts and the environmental uniqueness were significant predictors of taxonomic and functional richness, but only flow intermittence was an important predictor of taxonomic LCBD. For the IR-only data subset, disconnected pool permanence was the main predictor explaining spatiotemporal patterns. Our results highlight the importance of IRs to biodiversity conservation of Mediterranean climate rivers, especially during the disconnected pool phase, suggesting that these ecosystems cannot be ignored in conservation planning strategies.
A Fixed Margin Based Proximal Ordinal Support Vector Regression Model
Authors: Yao Xiao 
Published: August 2023

Abstract

This study extends the proximal support vector machine (PSVM) to the ordinal regression problems by proposing a proximal ordinal support vector regression (POSVR) based on the fixed margin strategy. The proposed POSVR is applied to the multi-class classification problems with ordered discrete outcome events under the assumption that the ordered classes can be separated by a group of parallel decision hyperplanes. POSVR can be transformed into a linear equation system and solved conveniently. The experimental results on the benchmark datasets and a real-world recovery rate dataset of US corporate bonds demonstrate that POSVR achieves better performance in terms of cross-validation outputs on the training set and out-of-sample prediction on the testing set. It implies that the proposed POSVR is a promising technique for credit risk modeling and other areas in ordinal regression problems.