bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patients and psychotherapists using concessive counter‐argumentation: Co‐constructing new framings
Authors: Luciani Margherita , Convertini Josephine 
Published: February 2023

AbstractShifting point of view and imagining alternative scenarios can be considered crucial goals of every therapeutic path. Reframing is aimed at stimulating other hypotheses and new viewpoints of the same situation and at adding complexity to one's own and to the others' world images. An interesting aspect to be investigated is the linguistic mechanisms through which this reframing can be enacted. Our work has an interdisciplinary focus and can be staged in between theories and techniques of psychotherapy on the one side and argumentation theory on the other side. The aim of this contribution was to explore the potential role that concessive counter‐argumentation plays in amplifying and redefining patients' framing. Our study consists of an in‐depth single‐case analysis based on two psychotherapeutic sessions. The analysis comprises different steps: first, we identified the concessive sentences in the transcriptions of the psychotherapy sessions and we considered them as counter‐argumentations; second, we identified the two parts of the concessive counter‐argumentations; third, we identified the inferences that could be drawn from these two parts; and finally, we reconstructed the contextual framing that was enacted and endorsed by both the patient and the therapist. The findings show that both the therapist's and the patient's concessive counter‐argumentations help in detecting key passages of sessions that open new, previously unimagined scenarios, thereby also contributing to clinical theory.
The impact of customer engagement on customer happiness
Authors: de Azambuja Gina María Pipoli , Rodriguez Peña Gustavo Adolfo , Vargas Enver Gerald Tarazona 
Published: February 2023

AbstractPrevious studies have found relationships between variables that predict happiness and engagement. While this suggests that engagement is a basis for generating customer happiness, the literature does not provide sufficient confirmatory evidence. Thus, the effects of different engagement intensities remain unclear. Focusing on conditions related to the Starbucks brand, this study analyzed how (1) passive and active engagement impacted customer happiness and (2) happiness impacted word of mouth (WOM) and purchase intention. Data were collected through an online survey among 802 Peruvian customers, all of whom were recruited via non‐probabilistic sampling. Because two exogenous variables were considered formative (i.e., customer engagement and customer happiness), a partial least‐squares structural equation model (PLS‐SEM) was used for the analysis. In sum, engagement (both passive and active) significantly and positively impacted customer happiness, which then positively impacted WOM and purchase intention. Given that engagement is associated with variables that are broadly related to happiness, this study makes new contributions by (i) clarifying how customer engagement can generate customer happiness, (ii) identifying differences between active and passive engagement, and (iii) adding evidence for use in the debate on the purpose of marketing and traditional practices such as engagement. As discussed in the paper, these findings have theoretical and practical implications for brand managers.
Synthesis, Biological Evaluation, and Molecular Modeling Studies of New 1,3,4‐Thiadiazole Derivatives as Potent Antimicrobial Agents
Authors: Acar Çevik Ulviye , Celik Ismail , İnce Ufuk , Maryam Zahra , Ahmad Iqrar , Patel Harun , Özkay Yusuf , Asım Kaplancıklı Zafer 
Published: February 2023

AbstractIn this work, the synthesis, characterization, and biological activities of a new series of 1,3,4‐thiadiazole derivatives were investigated. The structures of final compounds were identified using 1H‐NMR, 13C‐NMR, elemental analysis, and HRMS. All the new synthesized compounds were then screened for their antimicrobial activity against four types of pathogenic bacteria and one fungal strain, by application of the MIC assays, using Ampicilin, Gentamycin, Vancomycin, and Fluconazole as standards. Among the compounds, the MIC values of 4 and 8 μg/mL of the compounds 3f and 3g, respectively, are remarkable and indicate that these compounds are good candidates for antifungal activity. The docking experiments were used to identify the binding forms of produced ligands with sterol 14‐demethylase to acquire insight into relevant proteins. The MD performed about 100 ns simulations to validate selected compounds’ theoretical studies. Finally, using density functional theory (DFT) to predict reactivity, the chemical characteristics and quantum factors of synthesized compounds were computed. These results were then correlated with the experimental data. Furthermore, computational estimation was performed to predict the ADME properties of the most active compound 3f.
Bioisosterism: 1,2,4‐Oxadiazole Rings
Authors: Camci Merve , Karali Nilgün 
Published: February 2023

AbstractAlthough studies in drug discovery have gained momentum in recent years, the conversion of drugs in use today into less toxic derivatives with pharmacologically superior properties is still of great importance in drug research. Bioisosterism facilitates the conversion of drugs into derivatives that present more positive pharmacological and toxicological profiles by changing existing groups in the drug structure within the framework of certain criteria that have been expanded today. The 1,2,4‐oxadiazole ring is used as a bioisostere for ester and amide groups due to its resistance to hydrolysis. However, this ring is not limited to esters and amides, but can also be used as a bioisostere for other functional groups. In this review, cases in which the 1,2,4‐oxadiazole ring is used as a bioisostere for various functional groups are discussed. Herein we shed light on 1,2,4‐oxadiazole bioisosterism in the development of new drug candidates and in enhancing the pharmacological profiles of currently available drugs.
A prospective cohort study of decision‐making role preferences of patients with advanced cancer and their family caregivers
Authors: Ozdemir Semra , Ng Sean , Chaudhry Isha , Malhotra Chetna , Finkelstein Eric Andrew ,   COMPASS Study Group
Published: February 2023

AbstractBackgroundThis study aimed to examine (1) the evolution of patients–caregiver dyad decision‐making role preferences over 3 years and the predictors of these preferences; and (2) discordance in decision‐making role preferences among dyads.MethodsA total of 311 patients with advanced solid cancer and their caregivers in Singapore reported their preferences for decision‐making roles every 3 months. The predictors for decision‐making role preferences among dyads were identified via the actor–partner interdependence framework using a mixed‐effect ordered logistic model.ResultsThe proportion of patients and caregivers preferring patient‐led decision‐making was higher at the end of third year compared to baseline (patients: 40% vs. 20%, p value <.01; caregivers: 33% vs. 21%, p value = .03). Patients with female (odds ratio [OR], 1.74; p value <.01) and older (1‐year OR, 1.02; p value <.01) caregivers and younger patients (1‐year OR, 0.97; p value <.01) preferred higher involvement in decision‐making. Caregivers with tertiary education (vs. lower education) (OR, 1.59; p value = .02) and those who accurately understood patients' treatment goals (OR, 1.37; p value = .01) preferred greater patient involvement in decision‐making. Conversely, caregivers of female patients (OR, 0.68; p value = .03) and younger patients (1‐year OR, 0.98; p value <.01) preferred lesser patient involvement in decision‐making. The proportion of patient‐caregiver dyads with discordance in preferred decision‐making was lower at the end of the third year (51%) compared to baseline (68%) (p value <.01).ConclusionDespite a reduction in the proportion of dyads with discordance toward the end‐of‐life, the percentage with discordance remained high throughout the illness trajectory. Interventions facilitating open communication between dyads should be pursued in efforts to decrease dyadic discordance.
Utilizing mixture design response surface methodology to determine effective combinations of plant derived compounds as prostate cancer treatments
Authors: Berlin Ian Geddes , Jennings Charity Conlin , Shin Spencer , Kenealey Jason 
Published: February 2023

AbstractBackgroundProstate cancer (PC) is estimated to cause 13.1% of all new cancer cases in the United States in 2021. Natural bioactive compounds have drawn the interest of researchers worldwide in their efforts to find novel treatments for PC. Many of these bioactive compounds have been identified from traditional Chinese medicine (TCM) remedies often containing multiple bioactive compounds. However, in vitro studies frequently focus on the compounds in isolation.AimWe used mixture design response surface methodology (MDRSM) to assess changes in PC cell viability after 48 h of treatment to identify the optimal mixture of all 35 three‐compound combinations of seven bioactive compounds from TCM.Methods and ResultsWe used berberine, wogonin, shikonin, curcumin, triptolide, emodin, and silybin to treat PC3 and LNCaP human PC cells at their IC50 concentrations that we calculated. These compounds modulate many chemotherapeutic pathways including intrinsic and extrinsic apoptosis, increasing reactive oxygen species, decreasing metastatic pathways, inhibiting cell cycle progression. We hypothesize that because these compounds bind to unique molecular targets to activate different chemotherapeutic pathways, they will act synergistically to decrease tumor cell viability. Results from MDRSM showed that two‐way combinations were more effective than three‐way or single compounds. Most notably wogonin, silybin, emodin and berberine responded well in two‐compound combinations with each other in PC3 and LNCaP cells. We then conducted cell viability tests combining two bioactive compound ratios with docetaxel (Doc) and found significant results within the LNCaP cell line. In particular, mixtures of berberine and wogonin, berberine and silybin, emodin and berberine, and emodin and silybin reduced LNCaP cell viability up to an average of 90.02%. The two‐compound combinations were significantly better than docetaxel treatment of LNCaP cells.ConclusionWithin the PC3 cells, we show that a combination of berberine, wogonin and docetaxel is just as effective as docetaxel alone. Thus, we provide new combination treatments that are highly effective in vitro for treating androgen‐dependent and androgen‐independent PC.
Low‐power temperature‐independent CMOS measurement circuits for resistive gas sensors
Authors: Goyret Juan P. , Cassani María V. , Carbonetto Sebastián H. , Garcia‐Inza Mariano A. 
Published: February 2023

SummaryIn this work, we present the design of three different circuits for the measurement of resistive gas sensors. Schematic designs of each approach are discussed and simulated using HSPICE in a commercial CMOS 180 nm technology node. Their physical layout is also implemented. The three designs output a pulsed voltage signal whose period is modulated by the resistance of the sensor. They also implement differential measurement techniques in order to reduce the effect of temperature variations. The first design uses an operational amplifier and a resistor to measure the sensor and transduce its signal; the second one employs a Wheatstone bridge with common‐mode feedback and a bulk‐input OTA; and the third one utilizes a current‐mode Wheatstone bridge built with a current‐conveyor 2. The designs consume between 1.8 and
W; their temperature coefficient is always between 60 and 305 ppm/° C; and their resolution is between 0.25% and 1%. The circuits are compatible with sensors of a wide resistance range, being the best one tolerant to resistances between 2 M
and 1 G
. Therefore, these designs are ideal for low‐power autonomous applications in environments where temperature is not controlled.
Real‐time intelligent classification of COVID‐19 and thrombosis via massive image‐based analysis of platelet aggregates
Authors: Zhang Chenqi , Herbig Maik , Zhou Yuqi , Nishikawa Masako , Shifat‐E‐Rabbi Mohammad , Kanno Hiroshi , Yang Ruoxi , Ibayashi Yuma , Xiao Ting‐Hui , Rohde Gustavo K. , Sato Masataka , Kodera Satoshi , Daimon Masao , Yatomi Yutaka , Goda Keisuke 
Published: February 2023

AbstractMicrovascular thrombosis is a typical symptom of COVID‐19 and shows similarities to thrombosis. Using a microfluidic imaging flow cytometer, we measured the blood of 181 COVID‐19 samples and 101 non‐COVID‐19 thrombosis samples, resulting in a total of 6.3 million bright‐field images. We trained a convolutional neural network to distinguish single platelets, platelet aggregates, and white blood cells and performed classical image analysis for each subpopulation individually. Based on derived single‐cell features for each population, we trained machine learning models for classification between COVID‐19 and non‐COVID‐19 thrombosis, resulting in a patient testing accuracy of 75%. This result indicates that platelet formation differs between COVID‐19 and non‐COVID‐19 thrombosis. All analysis steps were optimized for efficiency and implemented in an easy‐to‐use plugin for the image viewer napari, allowing the entire analysis to be performed within seconds on mid‐range computers, which could be used for real‐time diagnosis.
Effect of HepG2 cell 3D cultivation on the metabolism of the anabolic androgenic steroid metandienone
Authors: Gronert Anika , Zierau Oliver , Thieme Detlef , Keiler Annekathrin Martina 
Published: February 2023

AbstractThe elucidation of the metabolic fate of prohibited substances is crucial for the abuse detection. The human hepatocyte cell line HepG2 can be used to study biotransformation. In order to improve this in vitro model system, we compared the HepG2 spheroid generation using three different techniques: a forced floating, a scaffold‐free and a scaffold‐based method. We characterized the spheroids with regard to the expression levels of the proliferation marker Mki67, the liver‐specific marker albumin and biotransformation enzymes. Moreover, the metandienone metabolite pattern was comparatively analysed by high‐performance liquid chromatography mass spectrometry. With all three techniques, HepG2 spheroids were generated showing a degree of differentiation. The forced floating method resulted in very large spheroids (1 mm in diameter) showing signs of necrosis in the centre and a very low metandienone conversion rate. The spheroids formed by the two other techniques were comparable in size with 0.5 mm in diameter on average. Among the three different 3D cultivation methods, the HepG2 spheroids formed on Matrigel® as extracellular matrix were the most promising regarding biotransformation studies on anabolic androgenic steroids. Prospectively, HepG2 spheroids are a promising in vitro model system to study multidrug setups, drug–drug interactions and the biotransformation of other substance classes.
Correction of the urinary testosterone to epitestosterone ratio measurement in antidoping analyses by chromatographic and mass spectrometric techniques
Authors: Martínez Brito Dayamín , Vidal Teresa Correa , de la Torre Xavier , de Oca Porto Rodny Montes , Botrè Francesco 
Published: February 2023

AbstractThe ratio of testosterone (T) to epitestosterone (E) determined in urine samples is the main biomarker used to prove T and precursors abused by athletes. Analytically, the correction of the ratio is required by the World Anti‐Doping Agency. This work describes a series of experiments aimed to study when it is appropriate to correct the T/E ratio value, using different mass spectrometric techniques since only reports on GC‐MS exist. Analyses using external calibrators, controls, and routine samples were performed by three different techniques GC‐MS, GC‐MSn, and LC‐MSn. A statistical comparison of the T/E was performed after the application of two corrections previously published: Isotopic contribution peak area correction (corr_1) and use of a verified internal deuterated internal standard (TD3/ED3) correction (corr_2) and the ratio based on T and E concentrations. The use of external calibration samples introduces biases that influence not only the T and E concentrations but also the T/E ratio, even when both methods of correction are applied. The correction after applying corr_1 method barely contributed to the accuracy of the T/E calculation. Nevertheless, the application of the corr_2 method increased the accuracy between 5% and 6% when comparing theoretical and experimental T/E values. Finally, the best results were obtained by the ratio calculated directly from the estimated concentrations of T and E. Attention must be paid when the T/E is measured by LC‐MSn since different acquisition modes produced significantly different results, even in MS/MS when the same transition is used for T and E.
If we build it, will they come? Commentary on “Preventing eating disorders and disordered eating in high‐risk families”
Authors: Bulik Cynthia M. 
Published: February 2023

AbstractLevine and Sadeh‐Sharvit (2023) open the door to a logical and evidence‐based targeted prevention strategy adapted from the field of depression. Their proposal is likely to benefit parents who are dealing with their own eating disorders and disordered eating while simultaneously breaking the cycle of risk inherent in the intergenerational transmission of eating disorders. The approach honors the wishes of parents who desperately want to buffer their children from the pain they experienced with their own suffering and provides hope for reducing environmental exposures that could augment any genetic risk that children of affected parents may hold.
Initial ecological change in plant and arthropod community composition after wildfires in designated areas of upland peatlands
Authors: Kelly Ruth , Montgomery W. Ian , Reid Neil 
Published: February 2023

AbstractWildfires are an increasing concern due to rising temperatures and incidence of droughts associated with changing climate, poor land management, and direct human interference. Most studies of the impact of fire on temperate heathland and bog examined the consequences of controlled or prescribed burning. Less is known about the impacts of uncontrolled wildfires on sites designated for their conservation value. We examined the initial impact and short‐term trajectory (3.5 years) of cool temperate peatland plant and arthropod communities on designated upland sites in Northern Ireland following wildfires, that is, unplanned with respect to where and when they occur, severity, and duration. These near simultaneous wildfires were often due to a failure to control prescribed burns. Wildfires were associated with a loss of blanket bog and heath indicator species. Broad vegetation groups showed initial recovery characterized by a decrease in bare ground and increasing cover of shrub species and bryophytes. However, at a species level, Sphagnum spp and bryophyte communities, which are central to peatland ecosystem functioning, showed no sign of recovery to prefire composition. Rather, bryophyte communities became more divergent over the course of the study and were mainly characterized by increased abundance of the alien pioneer acrocarp Campylopus introflexus. Similarly, composition of arthropod communities (ground beetles and spiders) differed between burnt and unburnt areas and showed no evidence of a return to species composition in unburnt areas. The nationally rare beetle Carabus nitens was more common in the aftermath of wildfire. Synthesis. Whilst, long‐term recovery was not investigated, these short‐term changes suggest enduring detrimental impacts on the distinctive communities associated with peatlands, primarily through the loss of Sphagnum spp., affecting ecosystem services such as carbon sequestration and water and soil retention. It may not be possible to restore exact prefire species composition of plant and animal communities. We suggest a precautionary approach involving management of upland vegetation, public education, and vigilance, to prevent further wildfires and protect these key upland habitats.
A population in perpetual motion: Highly dynamic roosting behavior of a tropical island endemic bat
Authors: Aguillon Samantha , Le Minter Gildas , Lebarbenchon Camille , Hoarau Axel O. G. , Toty Céline , Joffrin Léa , Ramanantsalama Riana V. , Augros Stéphane , Tortosa Pablo , Mavingui Patrick , Dietrich Muriel 
Published: February 2023

AbstractAlthough island endemic bats are a source of considerable conservation concerns, their biology remains poorly known. Here, we studied the phenology and roosting behavior of a tropical island endemic species: the Reunion free‐tailed bat (Mormopterus francoismoutoui). This widespread and abundant species occupies various natural and anthropogenic environments such as caves and buildings. We set up fine‐scale monitoring of 19 roosts over 27 months in Reunion Island and analyzed roost size and composition, sexual and age‐associated segregation of individuals, as well as the reproductive phenology and body condition of individuals. Based on extensive data collected from 6721 individuals, we revealed a highly dynamic roosting behavior, with marked seasonal sex‐ratio variation, linked to distinct patterns of sexual aggregation among roosts. Despite the widespread presence of pregnant females all over the island, parturition was localized in a few roosts, and flying juveniles dispersed rapidly toward all studied roosts. Our data also suggested a 7‐month delay between mating and pregnancy, highlighting a likely long interruption of the reproductive cycle in this tropical bat. Altogether, our results suggest a complex social organization in the Reunion free‐tailed bat, with important sex‐specific seasonal and spatial movements, including the possibility of altitudinal migration. Bat tracking and genetic studies would provide additional insights into the behavioral strategies that shape the biology of this enigmatic bat species. The fine‐scale spatiotemporal data revealed by our study will serve to the delineation of effective conservation plans, especially in the context of growing urbanization and agriculture expansion in Reunion Island.