bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reference Gene Selection for Quantitative Real-Time PCR Analysis of Hymenopellis radicata under Abiotic Stress
Authors: Cao Luping , Zhang Qin , Miao Renyun , Zhao Xu , Ni Yanqing , Li Wensheng , Feng Rencai , Yang Delong 
Published: August 2023

Abstract
Housekeeping genes are genes that are expressed stably in organisms. It is essential to select appropriate reference genes prior to conducting gene expression in order to obtain accurate results. Hymenopellis radicata (H. radicata) is an edible fungus rich in protein and mineral elements, with high edible and medical value. And reference genes suitable for normalization of qRT-PCR data from this species have not been investigated. In this study, therefore, we selected 11 housekeeping genes common in biology (CYP, GAPDH, PP2A, RPL6, UBC, UBQ10, ACT, EF, RPB2, TUA, TUB). The expression levels of these housekeeping genes were measured in three different tissues (pileus, stipe and root) and six different abiotic stress treatments in mycelium. They were evaluated for expression stability using online tools (GeNorm, NormFinder, BestKeeper and RefFinder). This study is the first to screen the most suitable genes as internal references in H. radicata and analyze the expression levels of H. radicata genes under abiotic stress and in different tissues. This project provides a reference for further research on the molecular mechanism of H. radicata under abiotic stress. Additionally, the results of this study can be used to select internal reference genes for expression analysis in other species.
Adsorption of silver from aqueous solution with high capacity and selectively by using Ag(I) imprinted polymeric nanoadsorbent
Authors: KARAKOÇ Veyis 
Published: August 2023

Abstract

The selective separation of precious metals from complex wastewater is important issue in terms of protecting the environment, aquatic ecosystems, water resources, and recycling of resources. Silver, which has been used for years in many fields from industry to health, enters the food chain and accumulates in living organisms by forming stable complexes, usually with sulfur containing molecules such as cysteine. In this study, cysteine-containing polymeric nanoparticles were synthesized by miniemulsion polymerization method by mimicking biological systems based on the affinity of silver to cysteine. The synthesized silver ion imprinted (Ag+-IIP) poly(Hydroxyethylmethacrylate-N-methacryloyl-L-cysteine) poly(HEMA-MAC) nanoparticles were characterized by Elemental analysiz zeta sizer AFM, SEM, FTIR. With elemental analysis study, the cysteine content of polymeric nanoparticles was determined to be 7.3 mmol MAC/g polymer. Molecular imprinting (MIP) technique was used to increase the selectivity of synthesized nanoparticles to Ag+ ions. The effects of pH, ionic strength and interaction time on the adsorption of Ag+ ions were investigated. To the experimental studies, the highest adsorption amount (196.9 mg/g nanoparticle) was reached in a short time like 40 minutes at pH:5 and 150mg/L concentration. Selectivity studies of synthesized Ag+-IIP nanoparticles to Ag+ ions were carried out in the presence of Li+, Ba2+, Hg2+ and Cd2+ ions, and it was observed that the nanoparticles adsorbed Ag+ ions with high selectivity. Ag+-IIP nanoparticles have 3.7, 3.1, 2.4 and 2.6 times more selective or higher affinity for Ag+ ions compared to Li+, Ba2+, Hg2+ and Cd2+ ions, respectively, than Ag+-non-imprinted (NIP) nanoparticles. The reusability studies, showed that there was no significant decrease in the adsorption capacity of the nanoparticles after the adsorption-desorption process repeated 10 times.
Discovery of SNPs in lignin biosynthesis genes (CAD1, MYB1 and MYB2) and their association with wood density in teak
Authors: Bano Nuzhat , Mohammad Naseer , Ansari Mohammad Israil , Ansari Shamim Akhtar 
Published: August 2023

Abstract
Forest trees like teak (Tectona grandis L.f.) with their prolonged growth period are well suited for association mapping (AM) or linkage disequilibrium (LD) mapping. The specifically designed primers successfully amplified the targeted lignin biosynthesis genes (CAD1, MYB1 and MYB2) in 150 unrelated genotypes assembled at National Teak Germplasm Bank, Chandrapur, Maharashtra, India. SNPs in the targeted amplified gene sequences were discovered and their associations with the wood density trait performed, following GLM and MLM statistical models and Bonferroni correction. CAD1 gene and MYB1 and MYB2 transcription factors brought out 112 SNPs/11 indels that were employed for association with the wood density trait with a mean value of 416.69kg/m3 (CV 4.97%). The teak genotypes comprised three admixed cryptic (K = 3) loosely structured sub-populations, harbouring 91.06% genetic variation within sub-populations and low sub-population differentiation (Fst = 0.03) with low intragenic LD (0–21% SNP pairs) at P < 0.05 and high LD decay (33bp-934bp) at R2 = 0.1 GLM and MLM models discounting systematic biases Q and K matrices to avoid false discovery revealed 5–7 loci at MAF < 0.003 and 3 loci at MAF > 0.05 to be significantly (P < 0.05) associated with wood density trait. However, Bonferonni correction (4.06–7.04×10− 4) yielded a single associated locus (B1485C/A) from exon of MYB1 transcription factor, contributing to about 12% phenotypic variation in wood density. The meticulously scored locus (B1485C/A) can be developed as a molecular probe for selection of improved planting stock with proven wood density trait for teak plantation.
Biomphalaria Glabrata (Say, 1818) as a Paratenic Host of Angiostrongylus Vasorum (Baillet,1866) Kamensky, 1905
Authors: Ávila Isabela Resende , de Oliveira Weder Gomes , Pereira Cíntia Aparecida de Jesus , Ferreira Mônica Alves Neves Diniz , Barçante Thales Augusto , Barçante Joziana Muniz de Paiva , Lima Walter dos Santos 
Published: August 2023

Abstract
Angiostrongylus vasorum parasitizes the pulmonary arteries and heart of dogs (definitive hosts - DHs), not ruling out a possible human infection (public health). Several species of molluscs participate as intermediate hosts (IHs), becoming infected by ingestion and/or penetration of first-stage larvae (L1), in which they undergo two molts L1, L2 and L3, which is infective for DHs. On the other hand, paratenic hosts (PHs), such as frogs, mice and birds, can harbor L3. The present study aimed to evaluate whether Biomphalaria glabrata could be PH of A. vasorum, identifying the possible penetration routes of these larvae, the migratory route, in addition to describing the histological changes at different times of infection. L3 were recovered from B. glabrata molluscs, 30 days after infection with 1,000 L1 of A. vasorum, using Baermann. The recovered L3 were used for infection of B. glabrata, with the number of 100 L3+ (L3 from mollusc infected with 100 L3). L3 + of A. vasorum were recovered alive and active in the tissues of molluscs, thus demonstrating their permissiveness to infection by L3+. The other infected molluscs were fixed in 10% formalin for histological evaluation, observing the presence of larvae in the tissue, with a tendency in the cephalopodal region. Regarding the viability of L3+, two mongrel dogs were submitted to canine pate with the presence of L3 + and L1 were found in the dogs' feces. This work demonstrates, for the first time, the possibility of B. glabrata participating as PH in the A. vasorum cycle.
Protective Efficacy of an IST DNA vaccine with a CL264 molecular adjuvant against Toxoplasma gondii in a murine model
Authors: Ju Kun-ping , Zhang Yun-nan , Xu Zhao-lin , Li Ling-yu , Zhou Huai-yu 
Published: August 2023

Abstract
Background
Toxoplasmosis, caused by the parasite Toxoplasma gondii (T. gondii), is a significant global zoonosis with devastating impacts. Despite its importance, an effective vaccine against toxoplasmosis for humans has not yet been developed. Vaccination remains the most cost-effective approach in combating this disease.
Methods
In this study, we designed and formulated a novel DNA vaccine encoding the inhibitor of STAT1 transcriptional activity (IST) of T. gondii utilizing the eukaryotic expression vector pEGFP-N1 for the first time. To enhance the immunoprotection of vaccine, we employed CL264, a TLR7 agonist, as a molecular adjuvant. The immunologic efficacy of this newly constructed vaccine was investigated in a murine model. Following intramuscular injection of the vaccine into mice, various indicators were assessed to evaluate the immune response, including antibodies, cytokines, and the proportion of CD4+ and CD8+ T cells. Additionally, two weeks after the third immunization, mice were challenged with highly virulent RH strain tachyzoites of T. gondii, and their survival time was observed.
Results
The vaccine demonstrated the ability to induce robust humoral and cellular immune responses in the immunized mice. Notably, TgIST-immunized mice exhibited prolonged survival time after T. gondii RH strain infection.
Conclusions
Our findings collectively demonstrate that the TgIST DNA vaccine elicits a significant immune response and offers partial protection against acute T. gondii infection in mice. These results suggest that TgIST holds potential as a candidate for further development as a DNA vaccine.
Enhanced Catalytic Activity of N-heterocyclic Carbene Stabilized Surface Adatoms for CO2 Reduction Reaction
Authors: Du Shixuan , Gao Yuxiang , Tao Lei , Zhang Yu-Yang 
Published: August 2023

Abstract
Adatom engineering represents a highly promising opportunity for enhancing electrochemical CO2 reduction reaction (CO2RR). However, the aggregation of adatoms under typical reaction conditions often leads to a decline in catalyst activity. Recent studies have revealed the N-heterocyclic carbene (NHC) can stabilize surface adatoms. Herein, based on density functional theory calculations, we reveal a significant enhancement in the catalytic activity of Cu adatoms decorated with NHC molecules for CO2RR. The NHC decoration strengthens the interaction between the dxy orbital of the Cu adatom and the px orbital of the C atom, reducing the energy barriers in both CO hydrogenation and C-C coupling steps. Moreover, the CO2RR catalytic activity of the NHC decorated adatom can be further improved by tuning the side groups of NHC molecules. These results provide new insights for the design of efficient CO2RR catalysts and offer a theoretical framework that can be extended to other hydrogenation reactions.
Dexmedetomidine combined with sufentanil in intravenous controlled analgesia after hip arthrotomy in children
Authors: He Zhiwei , Ni Huanhuan , Wang Wei 
Published: August 2023

Abstract
Background
To investigate the effect of different dose of dexmedetomidine combined with sufentanil on postoperative analgesia in children after hip arthrotomy.
Methods
The clinical data of 98 children who underwent hip arthrotomy in our center between January 2020 and February 2022 were selected. The children were divided into four groups according to the application of controlled analgesic drugs (sufentanil + granisetron ± dexmedetomidine).
Results
HR and RR in group C and D were more stable than those in group A and group B (P < 0.05). Pain scores decreased over time after treatment in all groups. When compared at the same time point, patients in Group D had the lowest pain scores, which were significantly lower than the other three groups (P < 0.05). The total consumption of sufentanil in group C and D was significantly lower than that in group A (P < 0.05). On 1st day after surgery, the children in Group D had lower levels of serum ACTH, IL-6, and COR levels than those in Group A.
Conclusion
Dexmedetomidine combined with sufentanil for children children with hip arthrotomy postoperative analgesia effect is exact, reduces sufentanil consumption and reduces the incidence of adverse reactions of opioids.
Field method for determination of available phosphorus in soil by selective formation of protonated crystal violet dimer:12-molybdophosphoric acid [(R)22+ H + -12MPA] complex
Authors: Thangavel Shanmugam , Rastogi Lori , Venkateswarlu Gumma , Prasad Ankam Durga , Dash Kulamani 
Published: August 2023

Abstract
Phosphorus (P) is an important nutrient for agricultural soil and it accelerates eutrophication in environmental water. In aqueous medium, sensitive method for P determination by ion-pair (IP) of triphenylmethane (TPM) dyes with 12-molybdophosphoric acid (12-MPA) is hampered due to multiple IP complexes, overlapping of IP spectrum with unassociated dye and potential interference from silicate. 12-MPA (H3PMo12O40) ion associate with monovalent and protonated crystal violet ions [R+, R+H+, R+(H)22+] forming R+-H2PMo12O40 (violet), R+H+-HPMo12O40 (bluish green) and R+(H)22+-PMo12O40 (yellow) complexes with λmax at ~ 590, 610 and 450 nm respectively. In this developed method, R+H+ of R+H+-HPMo12O40, aggregate to dimerization induced by complexing ion (HPMo12O402−) forms (R)22+ H+.PMo12O40 (blue, 560 nm). This ion-pair complex is selectively utilized for visual detection of phosphorus. No dimer formation of unassociated R+H+ and disappearance of shoulder of characteristic CV absorption spectrum remove spectral overlap at 560 nm. R+H+ at 610 nm, double protonated R+(H)22+ and its IP complex [R+(H)22+.PMo12O40] at ~ 450 nm don’t interfere in the analysis. Rapid formation of (R)22+H+- PMo12O40 and double protonation of R+H+ into R+(H)22+ on interaction with sample solution containing molybdate ion removes silicate interference. This method is successfully applied for agricultural soil for determination of available P in Olsen, Morgan, Mehlich, Bray and Acetic acid extraction procedures. The results obtained by the developed method are validated by ion-chromatography (IC). Five distinct colour formation for 0, 0.25, 0.5, 1.0 and 2.0 mg/L of P offers infield screening of agricultural soil as low, medium, high and very high available P for fertilizer management.
Key quantitative trait loci controlling bolting tolerance in sugar beet
Authors: KURODA Yosuke 
Published: August 2023

Abstract
For nonseed crops reliant on roots, leaves, and stems, breeding bolting-tolerant varieties is vital, and understanding the genetic mechanism aids effective selection. In sugar beet (Beta vulgaris), which accumulates sugar in roots, sequencing variations of BvBTC1, the master gene controlling annual and biennial life cycles, is associated with bolting tolerance, but the effects have not been demonstrated. We conducted quantitative trait loci (QTL) analysis on two generations (F2:3 and F5:6) from diverse bolting-tolerant crosses. Over four years, using phenotypic and mainly amplified fragment length polymorphism-based genotypic data, we identified two consistent QTLs: qB2 and qB6. These loci, detected regardless of the survey year or generation, were found to be crucial for enhancing sugar beet’s bolting tolerance. qB2 on chromosome 2 exhibited the highest phenotypic variance (PVE; 41.9%–66.6%) and was attributed to BvBTC1 based on mapping and gene function. On chromosome 6, qB6 (PVE 7.8%–23.7%) was located near bolting-related genes, such as Bv_22330_orky and BvFL1, but the gene responsible for qB6 remains unclear owing to map information limitations. Overall, the key QTL qB2 and qB6 hold promise for advancing bolting tolerance in sugar beet, offering valuable insights for targeted breeding efforts.
WITHDRAWN: Intuitionistic Fuzzy Threshold Hypergraphs and their role in chasing fugitives with multi-bots
Authors: Kothandapani Myithili , Chandrasekar Nandhini 
Published: August 2023

Abstract
The main aim of this paper is to catch fugitives utilising intuitionistic fuzzy threshold hypergraph(IFTHG) models with robots that are linked with each other and all of the bots are connected to the main control system. The fugitives can be held by the bots while the police arrive in this simulated research. Since the possible escape routes of drivers are not recognized, it is difficult to gather effective results, for a police to chase them in a short span of time. Several surveillance bots be placed in a waiting posture in this technique, having to wait for a fresh instruction to posture themselves in a particular region. In the occurrence of an unfortunate event, bots are notified to a control room \& alarmed. An Intuitionistic Fuzzy Hypergraph(IFHG) is considered as an IFTHG, if it is possible to construct from an empty IFTHG by adding a dominating vertex repeatedly to an every hyperedge. In such a way, here the dominating vertex is considered as major control room and the transmission distance is considered while joining the robots to form IFTHG. Here the threshold value is the maximum capacity of main control system. This hypothetical situation seems intended to take part on every crowed area. As a result, the IFTHG models are perfectly suited to this scenario. Security bots would be alerted and transmit a message to the control room in the event of an unusual occurrence such as robbery, heist, gunshot or accident. After receiving the alarm, a support squad will be sent to the specific location to begin the pursuit of the fugitives.
2000 Mathematics Subject Classification: 05C65
A novel imprinted locus on bovine chromosome 18 homologous with human Chromosome 16q24.1
Authors: Huo Hao-Nan , Zhang Cui , Wang Kun , Chen Wei-Na , Zhang Yin-Jiao , Yu Wen-Li , Li Shu-Jing , Li Shi-Jie 
Published: August 2023

Abstract
Genomic imprinting is an epigenetic regulation mechanism in mammals resulting in the parentally dependent monoallelic expression of genes. Imprinting disorders in humans are associated with several congenital syndromes and cancers and remain the focus of many medical studies. Imprinted genes usually cluster on chromosomes and are regulated by different methylation regions (DMRs) located in imprinting control regions (ICRs) that control gene expression in cis. There is an imprinted locus on human chromosome 16q24.1 associated with congenital lethal developmental lung disease in newborns. Cattle is a better model organism for investigating human embryo development than mice. However, genomic imprinting on bovine chromosome 18, which is homologous with human chromosome 16 has not been systematically studied. In this study, we analyzed the allelic expressions of eight genes (CDH13, ATP2C2, TLDC1, COTL1, CRISPLD2, ZDHHC7,KIAA0513, and GSE1) on bovine chromosome 18. Three transcript variants of the ZDHHC7gene (X1, X2, and X5) showed maternal imprinting in bovine placentas. In addition, the monoallelic expression of X2 and X5 was tissue-specific. Five transcripts of the KIAA0513 gene showed tissue- and isoform-specific monoallelic expression. The CDH13, ATP2C2, and TLDC1 genes exhibited tissue-specific imprinting, however, COTL1, CRISLPLD2,and GSE1 escaped imprinting. Four DMRs, established after fertilization, were found in this region. Two DMRs were located between the ZDHHC7 and KIAA0513genes, and two were in exon 1 of the CDH13 and ATP2C2 genes, respectively. The results from this study support future studies on the physiological roles of candidate genes on bovine chromosome 18.
Comparative Omics Analysis for Novel Target Discovery in Plant Pathogens: A Case Study for Magnaporthe oryzae
Authors: Luthfi Muhammad , Piapukiew Jittra , Pandey R. B. , Sompornpisut Pornthep 
Published: August 2023

Abstract
The central concern surrounding chemical pesticide application is its potential adverse effects on non-target organisms. For fungal pathogens, the search for specific targets has been complicated by the similarities in pathways shared between these pathogens and humans. We present a comprehensive strategy, integrating comparative omics and bioinformatics, to pinpoint precise targets for fungicides effective against the fungal pathogen Magnaporthe oryzae(M. oryzae), responsible for rice blast disease. Our approach involves subtractive metabolic pathways, homology screening and target prioritization. Through subtractive metabolic analysis, we identified three unique M. oryzaepathways, distinct from human and rice. Non-redundant protein sequences were subsequently subjected to BLASTP screening against human and rice, as well as other databases from diverse organisms. Target subcellular localization was predicted using eight tools, including Artificial Intelligence and a deep learning method. A comprehensive examination of biological processes was conducted, including gene expression, protein-protein interactions, network enrichment, broad-spectrum activity, and physicochemical analysis. Glutamate 5-kinase (G5K) emerged as the prime candidate for targeted fungicide development, promising progress in precision-oriented solutions.
Culture, Preferences, and the Gender Gap in Financial Literacy
Authors: Davoli Maddalena , Rodríguez-Planas Núria 
Published: August 2023

Abstract
Using country of ancestry to merge a nationally representative US sample of 9,623 adults from 26 countries of ancestries with two datasets collecting women’s and men’s financial knowledge and preferences, we investigate both the role of culture and that of cultural preferences in explaining women’s higher financial illiteracy relative to that of men. We find that the higher the degree of gender convergence in financial knowledge in the country of ancestry, the higher the financial knowledge of women in the US relative to their male counterparts. After ruling out gender differences in cognitive and non-cognitive skills as potential mechanisms, we find that higher patience and lower altruism in the country of ancestry are associated with greater financial knowledge for men but not for women in the US. Once we remove any country-of-ancestry gender variation from these preferences, gender convergence in financial knowledge in the country of ancestry continues to be associated with women’s (relative and absolute) greater financial literacy in the US, underscoring that gender differences in financial literacy are socially constructed. This relative and absolute female improvement is particularly robust for knowledge related to inflation and risk-diversification.
JEL Codes: D14, E2, I22, Z10
Diffusion MRI Anomaly Detection in Glioma Patients
Authors: Weninger Leon , Ecke Jarek , Jütten Kerstin , Clusmann Hans , Wiesmann Martin , Merhof Dorit , Na Chuh-Hyoun 
Published: August 2023

Abstract
Diffusion-MRI measures molecular diffusion, which allows to characterize microstructural properties of the human brain. Gliomas strongly alter these microstructural properties. Delineation of brain tumors currently mainly rely on conventional MRI-techniques, which are known to underestimate tumor volumes in diffusely infiltrating glioma. We hypothesized that diffusion-MRI is well suited for tumor delineation and developed two different deep learning approaches. The first diffusion anomaly detection architecture is a denoising autoencoder, the second consists of a reconstruction and a discrimination network. Each model was exclusively trained on non-annotated diffusion-MRI of healthy subjects and then applied on glioma patients´ data. To validate these models, a state-of-the-art supervised tumor segmentation network was modified to generate groundtruth tumor volumes based on structural-MRI. Compared to groundtruth segmentations, a dice score of 0.67 ± 0.2 was obtained. Further inspecting mismatches between diffusion-anomalous regions and groundtruth segmentations revealed, that these colocalized with lesions delineated only later on in structural-MRI follow-up scans, but which were not visible at the time of recording. Anomaly- detection methods are suitable for tumor delineation in diffusion-MRI acquisitions and may further enhance brain imaging analysis by detection of occult tumor infiltration in glioma patients, which could improve prognostication of disease evolution and tumor treatment strategies.