bluuebunny/crossref_metadata_2025_split huggingface dataset:
FGFR1 is an important prognostic factor in oral leukoplakia and tongue squamous cell carcinoma
Authors: Mariz Bruno Augusto Linhares Almeida , Sales de Sá Raísa , Araújo Anna Luiza Damaceno , Fernandes Carla Isabelly Rodrigues , Mariano Fernanda Viviane , Santos‐Silva Alan Roger , Lopes Marcio Ajudarte , Vargas Pablo Agustin , de Almeida Oslei Paes , Kowalski Luiz Paulo , Jorge Jacks 
Published: December 2022

AbstractBackgroundFibroblast growth factor receptor 1 is a potential prognostic factor for tongue squamous cell carcinoma and is associated with oral epithelial dysplasia grade in oral leukoplakia.MethodsThirty cases of tongue squamous cell carcinoma and 30 cases of oral leukoplakia were analyzed. Fibroblast growth factor receptor 1 and phosphorylated Akt protein expression were analyzed by immunohistochemistry and quantified using a digital algorithm. Fibroblast growth factor receptor 1 gene amplification was analyzed by fluorescent in situ hybridization in the tongue squamous cell carcinoma cases.ResultsClinical appearance and dysplasia grade were correlated with oral leukoplakia malignant transformation. Oral leukoplakia cases presenting high fibroblast growth factor receptor 1 expression showed a higher risk of malignant transformation (p = 0.016, HR: 7.3, 95% CI: 1.4–37.4). Phosphorylated Akt showed faint to no expression in oral leukoplakia, which did not correlate with dysplasia grade or malignant transformation. High expression of fibroblast growth factor receptor 1 and phosohorylated Akt were associated with poor overall survival and disease‐free survival in tongue squamous cell carcinoma, although only fibroblast growth factor receptor 1 expression was significantly associated with poor overall survival (p = 0.024; HR: 4.9, 95% CI: 1.2–19.9). Cases presenting double fibroblast growth factor receptor 1/phosphorylated Akt overexpression (n = 8) showed markedly impaired overall survival (p = 0.020; HR: 6.4, 95% CI: 1.3–31.1) and disease‐free survival (p = 0.001, HR: 13.0, 95% CI: 3.0–55.7). Fibroblast growth factor receptor 1 amplification was observed in 16.6% of tongue squamous cell carcinoma cases, being correlated with vascular and neural invasion (p = 0.001 and 0.017, respectively), but not with fibroblast growth factor receptor 1 protein expression, overall survival, or disease‐free survival.ConclusionFibroblast growth factor receptor 1 protein expression is an important prognostic factor in oral leukoplakia and tongue squamous cell carcinoma.
Hostile home environment predicting early adolescent sexual harassment perpetration and potential school‐related moderators
Authors: Espelage Dorothy L. , Harper Christopher R. , Ingram Katherine M. , Basile Kathleen C. , Leemis Ruth W. , Nickodem Kyle K. 
Published: December 2022

AbstractUsing family systems theory, this longitudinal study of middle school youth examined the effects of abuse, family conflict, and sibling aggression on sexual harassment perpetration (N = 1563; Mage 11.2, 51% boys; 39% Hispanic, 29% Black, and 19% White). Boys reported more sexual harassment than girls; perpetration increased for both. The association between a hostile home environment and sexual harassment perpetration was moderated by school experiences. School belonging buffered effects of hostile home environment on baseline sexual harassment perpetration for boys who experienced abuse and White adolescents with high sibling aggression. Academic grades moderated change in perpetration over time, but effects differed by sex and race. It is important to understand how early violence exposures relate to sexual violence perpetration during early adolescence.
Emergency powers, anti‐corruption, and policy failures during the COVID‐19 pandemic in Puerto Rico
Authors: Atiles Jose 
Published: December 2022

AbstractThis paper explores how the use of emergency powers by the US and Puerto Rican governments exacerbated the impact of the COVID‐19 pandemic and manufactured the conditions for furthering the multilayered economic, legal, political, and humanitarian crisis affecting Puerto Rico since 2006. The paper discusses three cases. First, it examines how the multiple declarations of the state of emergency, and its constant renewals, produced contradictory public health policies. Since the start of the COVID‐19 pandemic in March 2020, the Puerto Rican government has issued over 90 executive orders aimed at addressing the emergency, producing an unclear, contradictory, and unequal emergency management policy. Second, the paper focuses on the impact of the passing of Law 35 on April 5, 2020, which imposed severe penalties on those who disobeyed executive orders. As a result, hundreds of Puerto Ricans were arrested, fined, and incarcerated for violating the issued order. Third, the paper studies how, citing the presence of corruption, the Puerto Rican government implemented anti‐corruption and anti‐fraud policies that made it more difficult for those most in need of it—mainly poor and racialized individuals, as well as immigrants and working women—to access Pandemic Unemployment Assistance. Thus, the paper argues that emergency policies designed to address the pandemic, punitive governance, and anti‐corruption and anti‐fraud policies undermined Puerto Rico's capacity to handle the pandemic, exacerbated its impact, and created an unequal recovery scenario.
Trends in prevalence of probable fibrotic non‐alcoholic steatohepatitis in the United States, 1999–2016
Authors: Ciardullo Stefano , Perseghin Gianluca 
Published: December 2022

AbstractData are limited on the epidemiology of fibrotic non‐alcoholic steatohepatitis (NASH). We applied the recently proposed Fibrotic NASH Index (FNI) to estimate trends in the prevalence of probable fibrotic NASH in the general United States population using data from the 1999–2016 cycles of the National Health and Nutrition Examination Survey. A total of 40 273 participants aged 20 years and older were included in the analysis. The prevalence of probable fibrotic NASH (FNI ≥0.33) in the overall population was 8.4% (95% CI 8.0–8.8), with higher values found in males and in Hispanic participants. It increased from 6.9% (95% CI 6.3–7.6) in 1999–2004 to 9.2% (95% CI 8.4–10.0) in 2011–2016. This positive trend was evident in both sexes and in participants with obesity and diabetes. Finally, it increased progressively with increasing age, body mass index and worse glucose tolerance. NASH prevalence is increasing in the general US population, synchronous with increasing rates of obesity and type 2 diabetes.
Alternate reservoir operating policies for Lake Victoria in East Africa: A framework for balancing transboundary and local water demands
Authors: Kikoyo Duncan , Wurbs Ralph , Ale Srinivasulu , Smith Patricia 
Published: December 2022

AbstractThe reliability and impact of several regulation policies on Lake Victoria in East Africa were investigated using the Water Rights Analysis Package (WRAP). Its reliability is determined by establishing the risk of policies failing to meet water demands. The impacts on the lake are evaluated by assessing the effect of policies for lake levels. Policies are based on (i) the lake's firm and safe water yields and (ii) dam turbine configurations at the lake's outlet. The present study demonstrated the applicability of WRAP in modelling large complex reservoirs and determined that constant release policies with a maximum limit of 1015 m3/s have low risks of failure and improve lake water‐level stability.
Measuring Writing Development and Proficiency Gains Using Indices of Lexical and Syntactic Complexity: Evidence From Longitudinal Russian Learner Corpus Data
Authors: KISSELEV OLESYA , SOYAN ROSSINA , PASTUSHENKOV DMITRII , MERRILL JASON 
Published: December 2022

AbstractLinguistic complexity serves as an important measure of second language (L2) writing development. Complexity indices, however, rarely feature in the studies of learner languages other than English. Additionally, L2 writing studies have been criticized for the lack of consistency in defining proficiency. The current study addresses these gaps by exploring writing development in a less commonly studied language (i.e., Russian) while paying attention to the operationalization of proficiency. We analyzed lexical and syntactic complexity indices in a corpus of essays written by learners at different levels at the beginning and the end of an instructional program. Our analyses demonstrate that 9 complexity indices (i.e., mean word length, type–token ratio, percentage of high‐frequency words, mean sentence length, clauses per sentence, syntactic depth, proportion of subordinate clauses, and proportion of relative clauses) changed significantly over the course of the program; the same 9 indices showed significant correlation with the initial curricular placement, and all—with the exception of clauses per sentence—modestly or highly correlated with the final rating on the proficiency test. The findings confirm the usefulness of complexity measures in tracking writing development in L2 Russian.
Metabolic and phenotypic plasticity may contribute for the higher virulence of Trichosporon asahii over other Trichosporonaceae members
Authors: de Andrade Iara Bastos , Figueiredo‐Carvalho Maria Helena Galdino , Chaves Alessandra Leal da Silva , Coelho Rowena Alves , Almeida‐Silva Fernando , Zancopé‐Oliveira Rosely Maria , Frases Susana , Brito‐Santos Fábio , Almeida‐Paes Rodrigo 
Published: December 2022

AbstractBackgroundThe Trichosporonaceae family comprises a large number of basidiomycetes widely distributed in nature. Some of its members, especially Trichosporon asahii, have the ability to cause human infections. This ability is related to a series of virulence factors, which include lytic enzymes production, biofilm formation, resistance to oxidising agents, melanin and glucuronoxylomannan in the cell wall, metabolic plasticity and phenotypic switching. The last two are poorly addressed within human pathogenic Trichosporonaceae.ObjectiveThese factors were herein studied to contribute with the knowledge of these emerging pathogens and to uncover mechanisms that would explain the higher frequency of T. asahii in human infections.MethodsWe included 79 clinical isolates phenotypically identified as Trichosporon spp. and performed their molecular identification. Lactate and N‐acetyl glucosamine were the carbon sources of metabolic plasticity studies. Morphologically altered colonies after subcultures and incubation at 37°C indicated phenotypic switching.Results and ConclusionThe predominant species was T. asahii (n = 65), followed by Trichosporon inkin (n = 4), Apiotrichum montevideense (n = 3), Trichosporon japonicum (n = 2), Trichosporon faecale (n = 2), Cutaneotrichosporon debeurmannianum (n = 1), Trichosporon ovoides (n = 1) and Cutaneotrichosporon arboriforme (n = 1). T. asahii isolates had statistically higher growth on lactate and N‐acetylglucosamine and on glucose during the first 72 h of culture. T. asahii, T. inkin and T. japonicum isolates were able to perform phenotypic switching. These results expand the virulence knowledge of Trichosporonaceae members and point for a role for metabolic plasticity and phenotypic switching on the trichosporonosis pathogenesis.
Gender and socioeconomic disparities in global burden of chronic kidney disease due to glomerulonephritis: A global analysis
Authors: Bai Jiang , Yang Jing‐Yuan , Di Jing‐Kai , Shi Ya‐Ru , Zhang Jun‐Rui , Zhou Yun 
Published: December 2022

AbstractAimTo investigate the gender and socioeconomic disparities in the global burden of chronic kidney disease (CKD) due to glomerulonephritis from 1990 to 2019.MethodsData were extracted from the global burden of diseases (GBD) 2019 study, including incidence, prevalence and disability‐adjusted life‐years (DALYs). Estimated annual percentage changes (EAPCs) were calculated to quantify the temporal trends in age‐standardized rate (ASR) of CKD due to glomerulonephritis. Paired t‐test, paired Wilcoxon signed‐rank test and Spearman correlation were performed to analyse the association and gender disparity in CKD due to glomerulonephritis.ResultsGlobally, incident cases of CKD due to glomerulonephritis increased 81% from 9 557 397 in 1990 to 17 308 071 in 2019. The age‐standardized incidence rate increased by 1.47 compared with 1990 and DALYs increased by 1.35 compared with 1990 (per 100 000). The number of patients with CKD due to glomerulonephritis in low‐middle SDI (3829917) and middle SDI (6268817) regions accounts for more than 55% of the total cases. CKD due to glomerulonephritis caused a higher burden including the incidence rate (p < .0001) and DALY rate (p < .0001) in men compared to women. The age‐standardized DALY rate was negatively correlated with SDI (ρ = −0.64, p < .001). In the analysis of risk factors for DALYs, male individuals had a larger burden of hypertension, high BMI and high sodium diet in the DALY rates than female subjects.ConclusionThe burden of CKD due to glomerulonephritis was more skewed towards developing and less developed economies and differed by gender, so certain nations should implement far more focused and targeted policies.
The DnaJ domain‐containing heat‐shock protein NAL11 determines plant architecture by mediating gibberellin homeostasis in rice (Oryza sativa)
Authors: Luo Lixin , Xie Yuelan , Yu Sijia , Yang Jing , Chen Sirong , Yuan Xi , Guo Tao , Wang Hui , Liu Yongzhu , Chen Chun , Xiao Wuming , Chen Zhiqiang 
Published: December 2022

Summary

Ideal Plant Architecture 1 (IPA1) is a key regulator of plant architecture. However, knowledge of downstream genes applicable for improving rice plant architecture is very limited.

We identified the plant architecture regulatory gene NARROW LEAF 11 (NAL11), which encodes a heat‐shock protein (HSP) containing a DnaJ domain. A promising rare allele of NAL11 (NAL11−923del−1552) positively selected in Aus cultivars was identified; this allele exhibited increased expression and generated relatively few tillers, thick stems, and large panicles, components of the ideal plant architecture (IPA). NAL11 is involved in regulating the cell cycle and cell proliferation. NAL11 loss‐of‐function mutants present impaired chloroplast development and gibberellin (GA) defects.

Biochemical analyses show that IPA1 directly binds to elements in the missing fragment of the NAL11−923del−1552 promoter and negatively regulates NAL11 expression. Genetic analyses support the hypothesis that NAL11 acts downstream of IPA1 to regulate IPA by modulating GA homeostasis, and NAL11 may be an essential complement for IPA1.

Our work revealed that avoidance of the inhibition of NAL11−923del−1552 caused by IPA1 represents a positive strategy for rescuing GA defects accompanied by the upregulation of IPA1 in breeding high‐yield rice.
Long‐lasting impact of chitooligosaccharide application on strigolactone biosynthesis and fungal accommodation promotes arbuscular mycorrhiza in Medicago truncatula
Authors: Volpe Veronica , Chialva Matteo , Mazzarella Teresa , Crosino Andrea , Capitanio Serena , Costamagna Lorenzo , Kohlen Wouter , Genre Andrea 
Published: December 2022

Summary

The establishment of arbuscular mycorrhiza (AM) between plants and Glomeromycotina fungi is preceded by the exchange of chemical signals: fungal released Myc‐factors, including chitooligosaccharides (CO) and lipo‐chitooligosaccharides (LCO), activate plant symbiotic responses, while root‐exuded strigolactones stimulate hyphal branching and boost CO release. Furthermore, fungal signaling reinforcement through CO application was shown to promote AM development in Medicago truncatula, but the cellular and molecular bases of this effect remained unclear.

Here, we focused on long‐term M. truncatula responses to CO treatment, demonstrating its impact on the transcriptome of both mycorrhizal and nonmycorrhizal roots over several weeks and providing an insight into the mechanistic bases of the CO‐dependent promotion of AM colonization.

CO treatment caused the long‐lasting regulation of strigolactone biosynthesis and fungal accommodation‐related genes. This was mirrored by an increase in root didehydro‐orobanchol content, and the promotion of accommodation responses to AM fungi in root epidermal cells. Lastly, an advanced downregulation of AM symbiosis marker genes was observed at the latest time point in CO‐treated plants, in line with an increased number of senescent arbuscules.

Overall, CO treatment triggered molecular, metabolic, and cellular responses underpinning a protracted acceleration of AM development.
TheEPFL–ERf–SERKsignaling controls integument development in Arabidopsis
Authors: Li Meizhen , Lv Minghui , Wang Xiaojuan , Cai Zeping , Yao Hongrui , Zhang Dongyang , Li Huiqiang , Zhu Mingsong , Du Wenbin , Wang Ruoshi , Wang Zhe , Kui Hong , Hou Suiwen , Li Jia , Yi Jing , Gou Xiaoping 
Published: December 2022

SummaryAs the seed precursor, the ovule produces the female gametophyte (or embryo sac), and the subsequent double fertilization occurs in it. The integuments emerge sequentially from the integument primordia at the early stages of ovule development and finally enwrap the embryo sac gradually during gametogenesis, protecting and nursing the embryo sac. However, the mechanisms regulating integument development are still obscure.In this study, we show that SOMATIC EMBRYOGENESIS RECEPTOR‐LIKE KINASES (SERKs) play essential roles during integument development inArabidopsis thaliana. Theserk1/2/3triple mutant shows arrested integuments and abnormal embryo sacs, similar defects also found in the triple loss‐of‐function mutants ofERECTAfamily (ERf) genes. Ovules ofserk1/2/3 er erl1/2show defects similar toer erl1/2andserk1/2/3.Results of yeast two‐hybrid analyses, bimolecular fluorescence complementation (BiFC) analyses, and co‐immunoprecipitation assays demonstrated that SERKs interact with ERf, which depends on EPIDERMAL PATTERNING FACTOR‐LIKE (EPFL) family small peptides. The sextuple mutantepfl1/2/3/4/5/6shows integument defects similar to both ofer erl1/2andserk1/2/3.Our results demonstrate that ERf–SERK‐mediated EPFL signaling orchestrates the development of the female gametophyte and the surrounding sporophytic integuments.
Noncell‐autonomous HSC70.1 chaperone displays homeostatic feedback regulation by binding its own mRNA
Authors: Yang Lei , Zhou Yuan , Wang Shuangfeng , Xu Ying , Ostendorp Steffen , Tomkins Melissa , Kehr Julia , Morris Richard J. , Kragler Friedrich 
Published: December 2022

Summary

The HSC70/HSP70 family of heat shock proteins are evolutionarily conserved chaperones involved in protein folding, protein transport, and RNA binding. Arabidopsis HSC70 chaperones are thought to act as housekeeping chaperones and as such are involved in many growth‐related pathways. Whether Arabidopsis HSC70 binds RNA and whether this interaction is functional has remained an open question.

We provide evidence that the HSC70.1 chaperone binds its own mRNA via its C‐terminal short variable region (SVR) and inhibits its own translation.

The SVR encoding mRNA region is necessary for HSC70.1 transcript mobility to distant tissues and that HSC70.1 transcript and not protein mobility is required to rescue root growth and flowering time of hsc70 mutants. We propose that this negative protein‐transcript feedback loop may establish an on‐demand chaperone pool that allows for a rapid response to stress.

In summary, our data suggest that the Arabidopsis HSC70.1 chaperone can form a complex with its own transcript to regulate its translation and that both protein and transcript can act in a noncell‐autonomous manner, potentially maintaining chaperone homeostasis between tissues.
Pectin modifications at the symbiotic interface
Authors: Su Chao 
Published: December 2022

SummaryPlant cells are surrounded by a structured cell wall, which not only defines cell shape but also provides a structural barrier for protection against pathogen infection. However, the presence of this barrier does not impede the establishment of mutualistic symbioses between plants and several microbes (e.g. ectomycorrhizal fungi, arbuscular mycorrhizal fungi, and rhizobia). To establish such beneficial associations, symbiotic microbes need to colonize the plant tissues via intercellular and/or intracellular infection, a process that requires cell wall modifications. Although cell wall composition and changes during this process have interested researchers for years, the functional characterization of the molecular players involved is still limited. In this viewpoint, based on several new studies, I discuss how the PME‐PL/PG pathway mediates cell wall pectin modifications at the symbiotic interface and highlight further research directions which can broaden our understanding of how beneficial root symbioses are established.
Adenoid cystic carcinoma of submandibular gland: Emphasis on locoregional metastasis and prognosis
Authors: Liu Shengwen , Yang Jian , Lu Hao , Wu Yifan , Yang Wenjun , Xu Wanlin , Zhang Chenping 
Published: December 2022

AbstractObjectivesTo analyze the clinicopathological features, locoregional or distant metastasis, and prognosis of adenoid cystic carcinoma of submandibular gland (SMG‐AdCC).MethodsThe clinicopathological data of 80 patients with SMG‐AdCC from January 2005 to December 2017 were analyzed retrospectively, and the relationships between different parameters of SMG‐AdCC and its locoregional or distant metastasis or prognosis were analyzed.ResultsAs of December 2019, 41 patients (51.25%) were tumor‐free, while 20 patients were found to be living with tumors. The locoregional metastasis rate of grade II‐III SMG‐AdCC were found to be significantly higher than that of grade I. The five‐year DFS and OS rates were 70.8% and 87.1%, respectively. Univariate analysis showed that clinical size, extraglandular extension, pathological grade, pathological node (pN) status, and perineural invasion were correlated with DFS. Multivariate Cox regression analysis showed that pathological grade and extraglandular extension were independent prognostic factors for DFS; pN status and extraglandular extension were independent prognostic factors impacting OS.ConclusionThe pathological grade is a risk factor for locoregional metastasis of SMG‐AdCC. Pathological grade, pN status, and status of extraglandular extension are independent prognostic factors for DFS/OS in SMG‐AdCC patients.
Healthcare priorities in patients with chronic facial pain of temporomandibular disorders
Authors: Taimeh Dina , Riordain Richeal Ni , Fedele Stefano , Leeson Rachel 
Published: December 2022

AbstractObjectivesTo explore the experiences of patients with temporomandibular disorders (TMDs) with the National Health Service and to discover their healthcare priorities when seeking treatment.MethodsSemi‐structured interviews were used. They were directed using a topic guide covering subjects such as initial visits in primary care, referrals to secondary care, and the effect on symptoms. The discussions were audiotaped and transcribed verbatim. Thematic analysis was utilised to analyse the data.ResultsIn total, 15 participants took part in three focus groups. Six themes were identified: “access to appropriate care”, “organised and coordinated care”, “receiving a diagnosis and enough information”, “interaction with the clinical staff”, “treatment strategies and having an ‘action plan’” and “support and social networks”.ConclusionsThe participants gave accounts of the difficulties encountered in healthcare in general terms and specific to TMD. Most notable was the struggle to access appropriate care, receive a diagnosis and be understood. Our findings suggest that delays in delivering appointments with people of expertise may have caused the worsening of symptoms. However, when a pleasant experience was encountered, access to care was fast, the clinician was understanding and communication with the clinical team was good. These provided positive experiences and were appreciated by the patients.