bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-3154 promotes glioblastoma proliferation and metastasis via targeting TP53INP1
Authors: Lin Xiangdan , Wu Qiong , Lei Wei , Wu Dongyang , Sheng Jianchun , Liang Guobiao , Hou Guojun , Fan Di 
Published: December 2022

Abstract
Glioblastomas (GBM) are most common types of primary brain tumors and miRNAs play an important role in pathogenesis of glioblastomas. Here, we reported a new miRNA, miR-3154, which regulates glioblastoma proliferation and metastasis. miR-3154 was elevated in glioblastoma tissue and cell lines, and its elevation was associated with grade of glioblastomas. Knockdown of miR-3154 in cell lines weakened ability of proliferation and colony formation, and caused cell cycle arrested and higher percentage of apoptosis. Knockdown of miR-3154 also impaired ability of migration and invasion in glioblastoma cells. In mechanism, miR-3154 bound directly to Tumor Protein P53 Inducible Nuclear Protein 1 (TP53INP1), down-regulating TP53INP1 expression at both mRNA and protein level. Silence of TP53INP1 reversed the effect of miR-3154 knockdown on proliferation and metastasis of glioblastoma cells. These findings show that miR-3154 promotes glioblastoma proliferation and metastasis via targeting TP53INP1.
Development of a hypercoagulable-hypofibrinolytic state early after spinal cord injury
Authors: Park Andrew , Garcia Vinicius , Greiner Jared , Berry Auburn , Cardenas Hannah , Wegerson Kendra , Stauffer Brian , DeSouza Christopher 
Published: December 2022

Abstract

Study Design: Cross sectional study.
Objectives: To determine whether spinal cord injury (SCI) is associated with adverse changes in coagulation and fibrinolytic factors that underlie thrombogenesis and contribute to atherothrombotic events such as myocardial infarctions (MI) and strokes.
Setting: Adults with subacute SCI and non-injured community dwelling adults.
Methods: Thirty young and middle-aged (20-58 years) adults were studied: 14 non-injured (11M/4F) and 16 with subacute SCI (13M/3F; time since injury: 11.8±5.3 wk). Circulating markers of coagulation [von Willebrand factor (vWf) and factors VII, VIII, and X], the fibrinolytic system [tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitor-1 (PAI-1) antigen and activity], and fibrin formation (D-dimer) were determined by enzyme immunoassay.
Results: Circulating levels of coagulation factors VII, VIII and X were significantly higher (~20-45%) in the adults with SCI than non-injured adults; whereas, vWf was similar between groups. Fibrinolytic markers were adversely disrupted with SCI with t-PA antigen, PAI-1 antigen and PAI-1 activity were markedly higher (~50-800%; P<0.05) in adults with SCI compared with non-injured adults. The molar concentration ratio of active t-PA to PAI-1 was significantly higher (~350%) in adults with SCI. Concordant with coagulation cascade activation and fibrinolytic system inhibition, D-dimer concentrations were markedly ~70% higher (P<0.05) in adults with SCI compared with non-injured adults.
Conclusions: Subacute tetraplegic motor complete SCI is associated with a prothrombotic hemostatic profile. Adverse changes in the coagulation cascade and fibrinolytic system appear to occur early after injury and may contribute to the increased atherothrombotic risk in adults living with SCI.
Prognostic impact of blood urea nitrogen to albumin ratio on patients with sepsis: A retrospective cohort study
Authors: WANG Yuhe , GAO Shan , HONG Lei , HOU Tingting , LIU Huihui , LI Meng , ZHANG Yong 
Published: December 2022

Abstract
Objective
To investigate the ability of the ratio of blood urea nitrogen (BUN) to serum albumin ratio (BAR) in patients with sepsis in intensive care units (ICUs) to predict the prognosis of short-and long-term death.
Methods
Data were derived from the Medical Information Market in the Intensive Care IV (MIMIC-IV v2.0) database, with septic patients defined by SEPSIS-3. 30-day mortality for the primary outcome and 360-day mortality for the secondary outcome. Kaplan-Meier (KM) Survival curves were plotted to describe differences in BAR mortality in different subgroups, and area under the curve (AUC) analysis was performed to a comparison of BAR + SOFA and sequential organ failure assessment (SOFA) performance. Multivariate Cox regression models, restricted cubic spline curves (RCS), and subgroup analysis were used to ascertain the correlation between BAR and 30-day mortality and 360-day mortality.
Results
A total of 7656 eligible patients with a median BAR of 8.0 mg/g were enrolled in the study, with 3837 patients in the ≤ 8.0 group and 3819 in the BAR > 8.0 group, with 30-day mortality rates of (19.1% and 38.2%; P < 0.001)The area under the curve (AUC) was 0.718 (95% CI: 0.705–0.731) for SOFA + BAR and 0.703 (95% CI: 0.690–0.716) for SOFA. In the subgroup analysis, BAR remained an isolated risk element for patient death. For 360-day all-cause mortality, the same pattern was observed after adjustment for the same confounders.
Conclusion
As a clinically inexpensive and readily available parameter, BAR can be a valuable forecaster of prognosis in patients with sepsis in the intensive care unit.
Use of regression models for development of a simple and effective biogas decision-support tool
Authors: Duong Cuong Manh , Lim Teng-Teeh 
Published: December 2022

Abstract
Anaerobic digestion (AD) is an alternative way to treat manure while producing biogas as a renewable fuel. To increase the efficiency of AD performance, accurate prediction of biogas yield in difference working conditions is necessary. In this study, regression models were developed to estimate biogas production for co-digesting swine manure (SM) and waste kitchen oil (WKO) at mesophilic temperatures. Dataset was collected from the semi-continuous AD studies across nine treatments of SM and WKO, evaluated at 30, 35 and 40°C. Application of polynomial regression models and variable interactions with selected data resulted in an adjusted R2 value of 0.9656, much higher than the simple linear regression model (R2 = 0.7167). Significance of the model was observed with the mean absolute percentage error score at 4.16%. Biogas estimation using the final model resulted in the difference between predicted and actual values from 0.2–6.7%, except one treatment which was 9.8% different than observed. A spreadsheet was created to estimate biogas production and other operation factors using substrate loading rates and temperature settings. This user-friendly program can be used as a decision-support tool for providing recommendations of some working conditions and estimation of the biogas yield under different scenarios.
Clinical relationship between maxillomandibular characteristics and temporomandibular disc conditions in female patients with a skeletal class III pattern
Authors: Suntornchatchaweach Supakorn , Hoshi Kenji , Okamura Kazutoshi , Yoshiura Kazunori , Soonklang Kamonwan , Takahashi Ichiro 
Published: December 2022

Abstract
Objectives: To analyze the relationship between maxillomandibular characteristics and the severity of temporomandibular disc displacement in female patients with a skeletal class III (SKIII) pattern.
Materials and Methods: Fifty-seven samples were included. Articular disc conditions were evaluated by magnetic resonance imaging, and 25 cephalometric variables on lateral and postero-anterior cephalograms were measured to determine their maxillomandibular characteristics. The samples were classified into three groups based on the articular disc conditions: (1) normal disc position (NDP), (2) disc displacement with reduction (DDwR), and (3) disc displacement without reduction (DDwoR). The relationship between the maxillomandibular characteristics according to disc condition was examined in both a basic statistical analysis and a multivariate analysis known as principal component analysis.
Results: Kruskal-Wallis and Dunn-Bonferroni tests indicated a significant between-group difference in the deviation of mandibular characteristics seen on the postero-anterior cephalogram. The menton deviation, ramal height asymmetry index, and total mandibular length asymmetry index were significantly larger in the DDwoR group than in the NDP and DDwR groups. Furthermore, using principal component analysis, it was possible to extract all of the cephalometric variables into eight principal components. Only the principal component related to mandibular asymmetry could distinguish the SKIII samples with DDwoR from those in the other groups.
Conclusions: There is a significant relationship between mandibular asymmetry and the severity of disc displacement, particularly DDwoR, in female patients with a SKIII pattern.
Clinical Relevance: Etiologic relationship between SKIII patients with asymmetric mandible and the severity of articular disc conditions was significantly indicated in this study.
Laparoscopic Construction of Hand-sewn Roux-en-Y Limb: A Safe and Effective Means for Total Laparoscopic Radical Pediatric Choledochal Cyst Excision
Authors: Cheng Jiwen , Yang Weili , Yu Qiang , Miao ChunLin , Liu Yao , Fu JiaLu , Li Peng 
Published: December 2022

Abstract
Background
Laparoscopic or robot-assisted surgery has become the main pediatric minimal invasive surgery for a choledochal cyst (CDC). However, the Roux-en-Y jejunal limb was created extracorporeally in most reports and intracorporeally in a few reports using an endoscopic stapler. This study aimed to validate the safety and effectiveness of laparoscope-assisted Roux-en-Y jejunal limb created intracorporeally in pediatric minimal invasive surgery for a choledochal cyst (CDC) by hand-sewing.
Methods
Patients diagnosed with CDC between January 2020 and April 2022 were included in this retrospective analysis. We collected data on clinical characteristics, operative details, time for performing hand-sewn jejunum-jejunum anastomosis, and postoperative outcomes.
Results
Hand-sewn laparoscope-assisted Roux-en-Y jejunal limb was performed successfully in 21 cases. The median age of the patient was 5.8 years. The median age at surgery was 3.3 ± 3.1 years (26 days–13.6 years). The types of cysts included 12 Ia, 7 Ic, and 2 IVa. The mean operative duration was (216.5 ± 25.2) min, intraoperative bleeding volume was7 ± 2.3 ml, average postoperative time to start oral intake was 2.1 ± 0.4 days, average postoperative drainage tube indwelling time was 2.6 ± 1.1 days, and postoperative hospital stay was 5.3 ± 1.1 days. The follow-up period was 6 to 25 months. Complications (including anastomotic stenosis or fistula, pancreatitis, cholangitis, or death) were not observed in any patients.
Conclusions
Total laparoscope-assisted construction of hand-sewn Roux-en-Y limb, excision of the cyst, and hepaticojejunostomy are feasible and safe in the treatment for pediatric CDC. It may also has the following advantages: rapid recovery of postoperative gastrointestinal function, short hospitalization, no age limit on the patient and no additional hospitalization costs.
Expanded screening for neonatal inherited metabolic disorders by tandem mass spectrometry in an eastern Chinese population
Authors: Yang Xin , Sun Chuntao , Zhang Peiying , Zheng Jun , Zhang Linxin , TANG Li , Wang Yumei , Lin Xiaofei 
Published: December 2022

Abstract
Objective
To analyze the newborn screening results, distribution characteristics and incidence rate for inherited metabolic disorders (IMD) by tandem mass spectrometry (MS/MS) in Huai'an.
Methods
Blood samples were collected from 97410 newborns born in Huai'an from June 2018 to December 2021. Amino acids, acylcarnitines, and succinylacetone in the blood were detected by non-derivatized MS/MS. Gene detection and gas chromatography-mass spectrometry (GC-MS) were carried out to diagnose positive neonates. Data were analyzed using descriptive statistics.
Results
From 2018 to 2021, the screening rate of inherited metabolic disorders detected using MS/MS in Huai'an increased from 21.15–99.53%.Twenty-five cases of inherited metabolic disease were diagnosed, and the overall incidence rate was 1/3896. Among them, there were 9 cases of disorders of amino acid metabolism(1/10823), 10 cases of disorders of organic acid metabolism(1/9741), and 6 cases of disorders of fatty acid oxidation(1/16235). The top three diseases with the highest incidence were phenylalanine hydroxylase deficiency(1/12176), methylmalonic acidemia(1/24352), and primary carnitine deficiency (1/24352). Among the 25 children clinically diagnosed with IMD, 84.00% (21 cases) underwent gene diagnosis. Biallelic mutations were identified in 21 children, which were inherited from their parents.
Conclusion
Our study suggests that newborn screening for IMD by tandem mass spectrometry is necessary, which could improve the quality of patient's life and reduce the burden on family and society. More attention should be paid to the early screening, diagnosis and treatment of neonatal genetic metabolic diseases.
Adipose precursor cell-associated markgene combined with single-cell sequencing with machine learning to build an obesity prediction model
Authors: Xu Yuanxin , Cheng Weilun , Wang Yujing , Hong Xincai , Zhou Weiyu , Wang Yanru , Guo Baoliang , Kuang Hongyu , Yan Shuang 
Published: December 2022

Abstract
Objective: The purpose of this study is to Identify genes associated with adipose precursor cells(APCs), and apply machine learning methods, such as the minimum absolute contraction and selection operator (LASSO) and SVM recursive feature elimination (SVM-rfe), to find large obesity markers.
Method: We obtained a single-cell RNA sequencing dataset GSE133486 and three APCs RNA chip datasets GSE 24883, GSE25401 and GSE156906 from GEO.The R package was applied to find common differential genes, and key genes were obtained by LASSO with SVM-ref.
Results: THBS2, VASN, VCAN, ALDH1A3,GLUL, SLC4A4 take participate in the formation of APCs.
Conclusion: This study found that changes in different genes can regulate the occurrence and development of obesity, and discusses the possibility of these genes in the clinic.
Prevalence and factors associated with meconium-stained amniotic fluid after emergency cesarean section: multicenter cross-sectional study in Ethiopia
Authors: tantu Temesgen , zewdu Dereje , melkamu Biruk , gunta Muluken 
Published: December 2022

Abstract
Background
Meconium is thick black-green fetal intestinal content starting from the early first trimester of gestation. Unfortunately, if it is released into the amniotic cavity due to any cause, it can be associated with neonatal mortality and morbidity.
Objective
To identify the factors associated with meconium-stained amniotic fluid among mothers after emergency cesarean section in multicenter cross-sectional study in Ethiopia from September 1, 2021, to 30, October 2021
Method
Institution based multicenter cross-sectional study was done prospectively through meticulous chart review and interview on 895 mothers who gave birth with emergency cesarean section. Data were entered using Epi data 7 and analyzed with SPSS 26. The association between independent variables and meconium-stained amniotic fluid was estimated using an odds ratio with 95% confidence intervals. The statistical significance of the association was declared at P-value < 0.05
Result
The prevalence of meconium-stained amniotic fluid is 31.4%. Gravidity (AOR 3.643; 95% CI: 1.215,10.921), time interval between decision to delivery (AOR 1.361; 95% CI: 0.424,4.365), eclampsia(AOR 8.022; 95% CI: 1.634,39.372), time taken from referring institution to managing institution (AOR 15.258; 95% CI: 1.591,146.328), obstructed labor (AOR 24.614; 95% CI: 6.073,99.766), cephalopelvic disproportion (AOR 2.640; 95% CI:1.002,6.950), fetal heart beat abnormality (fetal bradycardia AOR 2.068; 95%CI 0.997,4.292) (fetal tachycardia AOR 3.513; 95% CI:1.633,7.556) duration of labor(AOR 5.44; 95% CI: 1.355,9.782) and referral from health center(AOR 5.41; 95% CI: 2.053,14.272) are associated with MSAF whereas obstetric complications(AOR 6.820; 95% CI: 2.870,16.202), cesarean section scar (AOR 3.010; 95% CI: 1.344,6.740) are negatively associated.
Conclusion
Prevalence of meconium-stained amniotic fluid is relatively high for which intrapartum, obstetric and institution related factors are incriminated. Therefore, an improvement in the quality of antenatal and intrapartum care is strongly recommended; professional development at the health center, building the infrastructure, and strengthening the referral system is also suggested.
Surface features and feedback type affect formation of prototype or exemplar representations in the 5/5 category learning task
Authors: Liu Zhiya , Chen Hao , Feng Jianru , Seger Carol A. 
Published: December 2022

Abstract
Theories of category learning have typically focused on how the underlying category structure affects the category representations acquired by learners. However, there is limited research as to how other factors such as how the category structure is instantiated as stimulus features and how feedback is structured affect what representations are learned and utilized. Across three experiments we varied the surface appearance and type of feedback while holding category structure consistent. We used a novel “5/5” categorization task developed from the well-studied 5/4 task with the addition of one more stimulus to clarify an ambiguity in the 5/4 prototypes. We used multiple methods including computational modeling to identify whether participants categorized on the basis of exemplar or prototype representations. We found substantial differences when the same structure was instantiated as schematic robot-like stimuli and richer bee-like images, in that the former was characterized by use of prototypes and the latter by use of exemplars. We also compared standard correct/incorrect feedback with point-valued feedback for the bee stimulus set and found exemplar use in the former but greater prototype use in the latter. These results indicated that in addition to the underlying structure of categories, the appearance of the stimuli, and form of feedback may affect the strategies utilized and resulting representations during category learning.
Genetic diversity, population genetic structure and gene flow in the rare and endangered wild plant Cypripedium macranthos revealed by genotyping-by-sequencing
Authors: Wu Qi , Dong Shang , Zhao Yuxin , Yang Lei , Qi Xiujin , Ren Zhihe , Dong Shubin , Cheng Jin 
Published: December 2022

Abstract
Background: Genetic diversity, genetic structure, and gene flow in plant populations and their influencing factors are important in conservation biology. Cypripedium macranthos isone of the few wild orchids with high ornamental value in northern China. However, over the past decade, excessive collection, trading, tourism development, habitat fragmentation, deceptive pollination, and seed germination difficulties have all caused a sharp decline in the number of C. macranthos individuals and its population. In order to propose a scientific and effective conservation strategy, the genetic diversity, genetic structure and gene flow of the current CM population are urgent scientific issues to be clarified.
Results: Here, 99 individuals of C. macranthos from north and northeastChina were analyzed to evaluate the genetic diversity, gene flow among populations, and genetic structure by genotyping-by-sequencing. More than 68.44 Gbhigh-quality clean reads and 41,154 SNPs were obtained. Our data based on bioinformatics methods revealed that C. macranthos has lower genetic diversity, high levels of historical gene flow, and moderate-to-high genetic differentiation between populations. The gene migration model revealed that the direction of gene flow was mainly from northeast populations to north populations in China. The results of genetic structure analysis showed that 11 C. macranthos populations can be considered as two groups, and further divided into four subgroups. Moreover, the Mantel test detected no significant “Isolation by Distance” between populations.
Conclusions: Our study demonstrates that the present genetic diversity andgenetic structure of C. macranthos populations weremainly caused by biological characteristics, human interference, habitat fragmentation, and restricted gene flow. Finally, constructive measures, which can provide a basis for the proposal of conservation strategies, have been suggested.
Recognition and management of hemorrhaging in combination with emerging enterogenic sepsis during a hepatectomy：a case report
Authors: Chen Yingjie , Liao Yanling , Chen Xiaoying , Fan Hanliang , Lin Daoyi , Zheng Ting , Chen Xiaohui , Gong Cansheng , Gao Fei , Jiang Jundan , Zheng Xiaochun 
Published: December 2022

Abstract
BACKGROUND: Patients with hemorrhagic shock may develop emerging enterogenic sepsis due to damage to the intestinal mucosal barrier and translocation of intestinal bacteria and endotoxins caused by ischemic injury. Because of the dual effects of anesthesia state and hemorrhagic shock, perioperative emerging enterogenic sepsis is even more rare and insidious.
Case presentation: We reported a case of 56-year-old man who underwent right hepatectomy for intrahepatic bile duct stones. Severe hemorrhage occurred during the procedure and the hemodynamics neither improved nor worsened after rehydration therapy and vasoactive drug administration. Based on the patient's history and clinical presentation, a possible enterogenic sepsis was considered. After anti-infective treatment and hormone supplementation, the patient's circulation improved significantly and he had an uneventful recovery.
Conclusion: The possibility of emerging enterogenic sepsis in hemorrhagic shock must always be taken into consideration. Familiarity with the risk factors and pathophysiological alterations of enterogenic sepsis is a prerequisite for early recognition and sound clinical decision making.
Identification of Genetic Loci Associated with Five Agronomic Traits in Alfalfa Using Multi-Environment Trials
Authors: Lin Sen , Medina Cesar Augusto , Wang Guojie , Combs David , Shewmaker Glenn , Fransen Steve , Llewellyn Don , Norberg Steven , Yu Long-Xi 
Published: December 2022

Abstract
Yield is one of the most important agronomic traits in alfalfa breeding; however, this is a complex trait affected by genetic and environmental factors. In this study, we used multi-environment trials to test yield-related traits in a diverse panel composed of 200 alfalfa accessions and varieties. Phenotypic data of maturity (MSC), dry matter, plant height (PH), yield (Yi), and fall dormancy (FD) were collected in three locations: Idaho, Oregon, and Washington from 2018 to 2020. Single-trial and stagewise analyses within each environment were used to obtain estimated means by genotype by trait by environment. Genome-wide association studies identified a total of 84 non-redundant markers associated with the traits analyzed. Of those, 55 markers were located at 44 different loci. Ten significant SNPs at the same locus were associated with FD and they were linked to a gene annotated as a nuclear fusion defective 4-like (NFD4) protein. SNPs linked to transcription factors such as Cysteine3Histidine (C3H), Hap3/NF-YB family, and serine/threonine-protein phosphatase 7 proteins were associated with MSC, PH, and Yi, respectively. Our results provide insight into the genetic factors that influence alfalfa maturity, yield, and dormancy, which is helpful to speed up the genetic gain of alfalfa yield improvement.