bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early life changes in histone landscape protect against age-associated amyloid toxicities through HSF-1 dependent regulation of lipid metabolism
Authors: Jakob Ursula , Oleson Bryndon , Bhattrai Janakraj , Zalubas Sarah , Kravchenko Tessa , Ji Yuanyuan , Jiang Emily , Lu Christine , Madden Ciara , Bazopoulou Daphne , Jones Jace 
Published: April 2023

Abstract
Recent studies revealed that early-in-life events can have highly beneficial long-term effects in animals. We now demonstrate that exposure of Caenorhabditis elegans to reactive oxygen species during development protects against amyloid-induced proteotoxicity later in life. We show that this protection is initiated by the inactivation of the redox sensitive H3K4me3 modifying COMPASS complex, and conferred by a substantial increase in the heat shock independent activity of heat shock factor 1 (HSF-1), a longevity factor known to act predominantly during C. elegans development. We show that depletion of HSF-1 leads to dramatic rearrangements of the organismal lipid landscape, and a significant decrease in mitochondrial β-oxidation. Both of these activities appear to majorly contribute to HSF-1’s protective effects against amyloid toxicity. In summary, our study reveals previously unknown links between developmental changes in the histone landscape, HSF-1 activity and lipid metabolism and the protection against age-associated amyloid toxicities later in life.
Using machine learning algorithm to analyse the hypothyroidism complications caused by radiotherapy in patients with head and neck cancer
Authors: Lee Tsair-Fwu , Lee Shen-Hao , Tseng Chin-Dar , Lin Chih-Hsueh , Chiu Chi-Min , Lin Guang-Zhi , Yang Jack , Chang Liyun , Chiu Yu-Hao , Yeh Shyh-An 
Published: April 2023

Abstract
Using machine learning algorithms to analyze the odds and predictors of complications of thyroid damage after radiation therapy in patients with head and neck cancer. This study used decision tree (DT), random forest (RF), and support vector machine (SVM) algorithms to evaluate predictors for the data of 137 head and neck cancer patients. Candidate factors included gender, age, thyroid volume, minimum dose, average dose, maximum dose, number of treatments, and relative volume of the organ receiving X dose (X: 10, 20, 30, 40, 50, 60 Gy). The algorithm was optimized according to these factors and cross-validation was performed 10 times to analyze the state of thyroid damage and select the predictors of thyroid dysfunction. The importance of the predictors identified by the three machine learning algorithms was ranked: the top five predictors were age, thyroid volume, average dose, V50 and V60. Of these, age and volume were negatively correlated with thyroid damage, indicating that the greater the age and thyroid volume, the lower the risk of thyroid damage; the average dose, V50 and V60 were positively correlated with thyroid damage, indicating that the larger the average dose, V50 and V60, the higher the risk of thyroid damage. The RF algorithm was most accurate in predicting the probability of thyroid damage among the three algorithms optimized using the above factors. The AUC was 0.827 and the ACC was 0.824. This study found that five predictors (age, thyroid volume, mean dose, V50 and V60) are important factors affecting the chance that patients with head and neck cancer who received radiation therapy will develop hypothyroidism. Using these factors as the prediction basis of the algorithm and using RF to predict the occurrence of hypothyroidism had the highest accuracy, which was 82.4%. This algorithm can be used as a reference for predicting the probability of radiation therapy complications and assisting medical decision-making in the future.
RNA-binding Protein ZC3H18 Enhances Cell Proliferation and Metastasis in Lung Adenocarcinoma
Authors: Mi Ke , Chen Yang , Zeng Lizhong , Yang Shuanying 
Published: April 2023

Abstract
Background LUAD is a prevalent type of lung cancer that originates from the glandular cells of the lung. Despite improvements in diagnosis and treatment, it remains a leading cause of cancer-related deaths worldwide. Thus, there is a critical need to develop reliable biomarkers for early detection, diagnosis, and prognosis of LUAD.Methods We first revealed that the expression level of ZC3H18 in LUAD patients. And we analyzed the prognostic value of ZC3H18 using KM-Plotter. We analyzed the co-expressed genes of ZC3H18 and conducted KEGG pathway and GO enrichment analyses. Furthermore, we conducted PPI network analysis, identified 8 hub genes closely associated with ZC3H18 to gain a better understanding of its functions. We silenced ZC3H18 using small interfering RNA (siRNA) and investigated its function in LUAD using in vitro functional assays. We measured cell proliferation through CCK-8, clone formation, and EdU assays, and evaluated cell migration and invasion abilities using scratch, Transwell migration, and invasion assays.Results ZC3H18 is highly expressed in LUAD and indicates poor prognosis. We screened 309 co-expressed genes and identified 8 hub genes (NOC2L, DDX51, DDX54, DHX37, SURF6, MYBBP1A, RBM19, POLR1A) closely related to RNA processes through PPI network analysis. Most of the enriched KEGG pathways and GO biological processes were also related to RNA processes. In vitro cell experiments showed that silencing ZC3H18 reduced cell proliferation and migration ability in LUAD.Conclusion The RNA-binding protein ZC3H18 promotes the proliferation and metastasis of LUAD through facilitating RNA processes.
Use of laryngeal mask airway in transferred infants: a retrospective study
Authors: Cavallin Francesco , Brombin Laura , Turati Marialuisa , Sparaventi Chiara , Doglioni Nicoletta , Villani Paolo Ernesto , Trevisanuto Daniele 
Published: April 2023

Abstract
Background Laryngeal mask airway (LMA) may be considered by health care givers of level I-II hospitals for neonatal resuscitation in interhospital care, but literature provides little information on this aspect. This study reviewed the use of LMA in a large series of neonates who underwent postnatal transfer by an Italian regional transport service.Methods This is a retrospective study evaluating the use of LMA in infants who underwent emergency transport by the Eastern Veneto Neonatal Emergency Transport Service between January 2003 and December 2021. All data were obtained from transport registry, transport forms and hospital charts.Results 64/3,252 transferred neonates (2%) received ventilation with a LMA, with increasing trend over time (p = 0.001). Most neonates were transferred after birth (97%) due to a respiratory or neurologic disease (95%). LMA was used before the transport (n = 60), during the transport (n = 1) or both (n = 3). No device-related adverse effects were recorded. Sixty-one neonates (95%) survived and were discharged/transferred from the referral center.Conclusions In a large series of transferred neonates, LMA use was rare but increasing over time, and showed some heterogeneity among referring centers. However, LMA was safe and lifesaving in “cannot intubate, cannot oxygenate” situations. Future prospective, multicenter research may provide detailed insights on LMA use in neonates needing postnatal transport.
Integrated bioinformatics and wet-lab analysis revealed cell adhesion prominent genes CDC42, TAGLN and GSN as prognostic biomarkers in colonic-polyp lesions
Authors: Tabatabaei Elmira , Mazloomnejad Radman , Rejali Leili , Forouzesh Flora , Noukabadi Fatemeh , Khanabadi Binazir , Salehi Zahra , nazemalhosseini-mojarad ehsan 
Published: April 2023

Abstract
Background Colorectal cancers are derived from intestinal polyps. Normally, alterations in cell adhesion genes expression cause deviation from the normal cell cycle, leading to cancer development, progression, and invasion. The present study aimed to investigate the elusive expression pattern of CDC42, TAGLN, and GSN genes in patients with high and low-risk polyp samples, and also colorectal cancer patients and their adjacent normal tissues.Materials and methods In upcoming study, 40 biopsy samples from Taleghani Hospital (Tehran, Iran) were collected, consisting of 20 colon polyps and 20 paired adjacent normal tissues. The expression of the nominated genes CDC42, TAGLN, and GSN was analyzed using quantitative polymerase chain reaction (Q-PCR) and relative quantification was determined using the 2-ΔΔCt method. ROC curve analysis was performed to compare high-risk and low-risk polyps for the investigated genes. The expression of adhesion molecule genes was also evaluated using TCGA data and the correlation between adhesion molecule gene expression and immunophenotype was analyzed. The role of mi-RNAs and lncRNAs in overexpression of adhesion molecule genes was studied. Lastly, GO and KEGG were performed to identify pathways related to adhesion molecule gene expression in healthy, normal adjacent, and COAD tissues.Results The results showed that the expression patterns of these genes were significantly elevated in high-risk adenomas compared to low-risk polyps and normal tissues and were associated with various clinicopathological characteristics. The estimated AUC for CDC42, TAGLN, and GSN were 0.87, 0.77, and 0.80, respectively. The study also analyzed COAD cancer patient data and found that the selected gene expression in cancer patients was significantly reduced compared to high-risk polyps and healthy tissues. Survival analysis showed that while the expression level of the GSN gene had no significant relationship with survival rate, the expression of CDC42 and TAGLN genes did have a meaningful relationship, but with opposite effects, suggesting the potential use of these genes as diagnostic or prognostic markers for colorectal cancer.Conclusion The present study's findings suggest that the expression pattern of CDC42, TAGLN, and GSN genes was significantly increased during the transformation of normal tissue to polyp lesions, indicating their potential as prognostic biomarkers for colorectal polyp development. Further
Minimizing shivering during targeted normothermia: Comparison between a Novel Trans-Nasal and Surface Temperature Modulating Devices
Authors: Arnold Shannon , Armahizer Michael , Torres Luis F. , Tripathi Hemantkumar , Tandri Harikrishna , Choi H Alex , Chang Jason J. , Badjatia Neeraj 
Published: April 2023

Abstract
Background: Shivering is a common adverse effect of achieving and maintaining normothermia in neurocritical care patients. We compared the burden of shivering and shivering-related interventions between a novel trans-nasal temperature modulating device(tnTMD) and surface cooling temperature modulating devices(sTMDs) during the first 24 hours of targeted normothermia in mechanically ventilated febrile neurocritical care patients.
Methods: This is a case: control study controlling for factors that impact shiver burden: age, sex, body surface area. All patients underwent transnasal cooling (COOLSTAT, KeyTech, Inc) as part of an ongoing multicenter clinical trial(NCT03360656). Patients undergoing treatment with sTMDs were selected from consecutively treated patients during the same time-period. Data collected included: core body temperature (every 2 hours), bedside shivering assessment scale(BSAS) score (every 2 hours), and administration of anti-shivering medication for BSAS>1.Time to normothermia(<=37.5 C), as well as temperature burden>37.5 C(C*hr) were compared between groups using student’s t-test for mean differences. Proportion of patients requiring interventions as well as number of interventions per patient were compared using Chi-Square test. Significance was determined based on a P value < 0.05.
Results: There were 10 tnTMD patients and 30 sTMD patients included in the analysis (mean age: 62+/-4, 30% women, BSA = 1.97+/-0.25). There were no differences between groups in temperature at cooling initiation (tnTMD: 38.5+/-0.2 C vs sTMD: 38.7+/-0.5 C, P=0.3), time to <=37.5 C (tnTMD: 1.8+/-1.5 hours vs. sTMD: 2.9+/-1.4 hours, P=0.1), or temperature burden >37.5 (tnTMD:: -0.4 +/- 1.13 C*hr vs. sTMD median (IQR): -0.57 +/- 0.58 C*hr, P=0.67). The number of tnTMD patients who received pharmacologic shivering interventions was lower than the controls (20%vs.67%,p=0.01). tnTMD patients also had fewer shivering interventions per patient (0 (range: 0-3)vs.4(range: 0 – 23 ), p<0.001).
Conclusion: A transnasal cooling approach achieved similar time to normothermia and temperature burden with less shivering than surface cooling. This approach may be a feasible option to consider for mechanically ventilated febrile neurocritical care patients.
Trial registration: Clinical trials.gov: NCT03360656. Registered December 4, 2017. https://clinicaltrials.gov/ct2/show/NCT03360656
Integrative multi-region molecular profiling of primary prostate cancer with synchronous lymph node metastasis to characterize the biologically dominant clone
Authors: Singhal Udit , Nallandhighal Srinivas , Tosoian Jeffrey , Pham Trinh , Stangl-Kremser Judith , Lui CJ , Karim Razeem , Plouffe Komal , Morgan Todd , Cieslik Marcin , Luciano Roberta , Shariat Shahrokh , Finocchio Nadia , Dambrosio Lucia , Doglioni Claudio , Chinnaiyan Arul , Tomlins Scott , Briganti Alberto , Palapattu Ganesh , Udager Aaron , Salami Simpa 
Published: April 2023

Abstract

Localized prostate cancer is composed of multiple spatially distinct tumors with significant inter- and intra-tumoral molecular heterogeneity. This genomic diversity gives rise to many competing subclones that may drive the biological trajectory of the disease. Previous large scale sequencing efforts have focused on the evolutionary process of metastatic prostate cancer, revealing a potential clonal progression to castration resistance. However, the clonal origin of synchronous lymph node (LN) metastases in primary disease is still unknown. Here, we performed multi-region, targeted DNA/RNA next generation sequencing (NGS) and constructed phylogenetic trees from 14 patients with LN metastasis (88 primary prostate cancer foci with 23 synchronous LN metastases) to better define the molecular features of primary disease most likely to spread to the LNs. Of eight primary prostate cancer cases with evidence of extra-prostatic extension (EPE), phylogenetic analysis supported this region as the likely source of LN metastasis in four cases. In two patients with organ-confined disease and LN metastasis, sub-clonal seeding and clonal evolution was observed, with LN metastasis likely arising from a Gleason Grade Group 5 focus. Cribriform pattern was observed in seven patients in both LNs and the primary tumor foci most clonally related. Driver alterations, either oncogenic gene fusions or somatic mutations (e.g., CDK12, FOXA1), were shared among primary tumor and LN metastatic foci. Collectively, we found that a combination of histopathologic and molecular factors, including tumor grade, EPE, cellular morphology (e.g., cribriform pattern), and oncogenic genomic alterations were associated with synchronous LN metastasis. More work is needed to better define the molecular features of primary prostate cancer foci most likely to give rise to metastasis to improve risk stratification, guide treatment allocation, and inform novel therapeutic strategies.
FEM analysis of a new three-way drainage and pressure reduction system for road tunnels
Authors: Teng Zhaolei , Liu Yuanming , Mei Shilong , Zhou Yuhang , He Guohua , Li Yingxiao , Du Bitao 
Published: April 2023

Abstract
For the water-rich area tunnel elevation arch in the high-water pressure often causes elevation arch cracking and leakage, bulging and other disasters. When the drainage system is not designed properly, such disasters occur more frequently, and conventional road tunnel drainage cannot effectively reduce the water pressure at the elevation arch. Therefore, this paper proposes a new concept of "three-way drainage". The three-way drainage system is based on the conventional drainage system with a new drainage inlet at the elevation arch. On this basis, a series of numerical simulation studies are conducted to verify the pressure-reducing performance of the three-way drainage system on the lining. After demonstration and analysis, the three-way drainage concept can not only effectively reduce the water pressure at the elevation arch of the tunnel, but also have a significant effect on the overall drainage effect of the tunnel. The factors affecting the performance of the three-way drainage system are discussed by varying the model parameters. It was found that the hydraulic conduction coefficient of the surrounding rock and initial support, the number of reverse diversion holes in the elevation arch, the change in head height and the change in secondary lining parameters all had a significant effect on the water pressure outside the tunnel.
Effects of museum-based art activities on older community dwellers’ physical activity: The A-health randomized controlled trial results
Authors: Planta Océane , Cami Margot , Matskiv Jacqueline , Plonka Alexandra , Gros Auriane , Beauchet Olivier 
Published: April 2023

Abstract
Purpose. Museum-based art activities have demonstrated health benefits in older adults. Few clinical trials, however, have examined physical health benefits. This randomized controlled trial (RCT) aims to compare changes in daily step count over a 3-month period in older adults participating in museum art-based activities and their control counterparts.
Methods. Using a subset of 53 participants recruited in the A-health RCT, the daily step count of 28 participants in the intervention group and 25 in the control group were recorded with a Fitbit Alta HR. Weekly art-based activities were carried out at the Montreal Museum of Fine Art (MMFA, Quebec, Canada) over a 3-month period. The outcomes were the mean step count per active hours (i.e., between noon and 6pm), inactive hours (i.e., between midnight and 6am) and full day (i.e., 24h), and the change of step count following the 3-month (M3) intervention of art activities at the MMFA (M3).
Results. The intervention group had greater daily step count compared to the control group at M3, regardless of the step parameters examined (P ≤ 0.026). The change in daily step count for active hours (P = 0.023) and full day (P = 0.011) increased significantly with the MMFA art-based activities.
Conclusion. MMFA-based art activities improved daily physical activity in older community-dwellers who participated in the RCT, confirming health benefits and suggesting a potential of museums in health promotion and prevention.
Transcriptome analysis of fresh-cut kiwifruit treated with slightly acidic electrolyzed water (SAEW) and 1-methylcyclopropene (1-MCP)
Authors: Xu Fangxu 
Published: April 2023

Abstract
Fresh-cut kiwifruit is difficult to store due to the characteristics of thin and juicy rind, which results in the great limitation of development and utilization. The aim of the study was to evaluate the effect of slightly acidic electrolyzed water (SAEW) combined with 1-methylcyclopropene (1-MCP) on shelf life quality of fresh-cut kiwifruit by transcriptome technology. The results showed that SAEW or 1-MCP treatment suppressed ethylene production, respiratory rate, soluble solid content (SSC), polyphenol oxidase (PPO) and improved titratable acid content (TAC), total phenols content (TPC), total chlorophyll content (TCC), peroxidase (POD) and phenylalanine ammonia lyase (PAL) activities comparing with the untreated samples. There were 2754 differentially expressed genes (DEGs) between the combined treatment and the control on day 6. GO function annotation results showed that the related genes involved in cell membrane composition in samples might be involved in protein phosphorylation under the action of protein kinase genes. KEGG enrichment analysis showed that 715 DEGs were significantly enriched in 111 metabolic pathways on day 6 after combined treatment. In ethylene signaling pathway, expression levels of ETR and ERF1 were down-regulated, which proved that 1-MCP inhibited ethylene reaction by binding to ethylene receptors. Genes expression levels of SnRK2, MPK3/6, and BAP31 related to MAPK signaling pathway and protein processing in endoplasmic reticulum pathway were up-regulated, and the combined treatment had a positive regulatory effect on fruit defense during storage. In plant-pathogen interaction pathway, genes expression levels of MKK45 were down-regulated that proved the production of ROS was suppressed by combined treatment, consequently improving disease resistance of fresh-cut kiwifruit. Hence, the combination treatment of SAEW and 1-MCP may be a proven and effective technology to retain higher quality and nutritive properties of fresh-cut kiwifruit.
Molecular characterization of a novel single-stranded RNA mycovirus infecting the plant pathogenic fungus Ustilaginoidea virens
Authors: Zeng Rui , He Zhenrui , Fan Yu , Huang Xiaotong , Yang Mei , Zhou Erxun 
Published: April 2023

Abstract
Mycovirus is a kind of virus that infects fungi and oomycetes and can replicate in them, which widespread in all major groups of plant-pathogenic fungi and oomycetes. To date, no positive-sense single-stranded RNA (+ ssRNA) virus has been reported to be associated with Ustilaginoidea virens, the notorious causal agent of rice false smut (RFS). Here, we report the molecular characterization of a novel + ssRNA mycovirus, Ustilaginoidea virens narnavirus 1 (UvNV1), isolated from U. virens strain Uv418. UvNV1 has a genome of 3,131 nt and possesses an open reading frame (ORF) predicted to encode an RNA-dependent RNA polymerase (RdRP) of 1,017 amino acids (aa) with a molecular mass of 116.6 kDa. BLASTp analysis revealed that the RdRP showed 50.34% aa similarity to that of the previously described Zhangzhou Narna tick virus 1. Phylogenetic analyses indicated that UvNV1 is highly similar to viruses taxonomically classified in the genus Narnavirus, family Narnaviridae. Taken together, these results clearly demonstrate that UvNV1 is the first + ssRNA virus known to infect U. virens.
Clarifying the composition of the ATP consumption factors required for maintaining ion homeostasis in mouse rod photoreceptors
Authors: Muangkram Yuttamol , Himeno Yukiko , Amano Akira 
Published: April 2023

Abstract
To date, no effective treatment has been established for photoreceptor loss due to energy imbalances, but numerous therapeutic approaches have reported some success in slowing photoreceptor degeneration by downregulating energy demand. However, the detailed mechanisms remain unclear. This study aimed to clarify the composition of ATP consumption factors in photoreceptors in darkness and in light. We introduced mathematical formulas for ionic current activities combined with a phototransduction model to form a new mathematical model for estimating the energy expenditure of each ionic current. The proposed model included various ionic currents identified in mouse rods using a gene expression database incorporating an available electrophysiological recording of each specific gene. ATP was mainly consumed by Na+/K+-ATPase and plasma membrane Ca2+-ATPase pumps to remove excess Na+ and Ca2+. The rod consumed 7x107 molecules of ATP s− 1, where 65% was used to remove ions from the cyclic nucleotide-gated channel and 20% from the hyperpolarization-activated current in darkness. Increased light intensity raised the energy requirements of the complex phototransduction cascade mechanisms. Nevertheless, the overall energy consumption was less than that in darkness due to the significant reduction in ATPase activities, where the hyperpolarization-activated current proportion increased to 83%. A better understanding of energy demand/supply may provide an effective tool for investigating retinal pathophysiological changes and analyzing novel therapeutic treatments related to the energy consumption of photoreceptors.