bluuebunny/crossref_metadata_2025_split huggingface dataset:
Community-associated methicillin-resistant Staphylococcus aureus infection of diabetic foot ulcers in an eastern diabetic foot center in China: A retrospective study
Authors: Chen Yixin , Yang Jie , Wang Ying , You Jiaxing , Zhu Weifen , Liu Chao , Li Lin , Li Hong 
Published: March 2023

Abstract
Background
Diabetic foot concerns are a major public health problem. Methicillin-resistant Staphylococcus aureus (MRSA) plays a significant role in diabetic foot ulcers (DFU). Community-associated MRSA (CA-MRSA) has become notorious for skin and skin soft tissue infections over the last two decades. This study investigated MRSA infection in DFU at a tertiary hospital, focusing on the epidemiology and characteristics of CA-MRSA.
Methods
A total of 149 patients with diabetic foot infection caused by Staphylococcus aureus were selected. Epidemiological investigations, clinical characteristics, laboratory index records, antibiotic susceptibility analysis, and clinical outcome tracking were performed in all cases. Based on oxacillin resistance, cases were divided into methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) groups. Subgroup analysis of the MRSA group was performed in accordance with the Centers for Disease Control definition: CA-MRSA and hospital-associated MRSA (HA-MRSA).
Results
Compared with the MSSA group (72.5%), the MRSA group (27.5%) had a longer duration of DFU, longer hospital stay, and higher hospitalization costs. The MRSA group was further subdivided into (CA-MRSA) and hospital-associated MRSA (HA-MRSA) groups. The severity of infection in the CA-MRSA group was higher than that in the HA-MRSA group. The analysis of antimicrobial susceptibility of 41 MRSA isolates showed that the resistance rates to erythromycin, clindamycin, quinolone, gentamicin, tetracycline, and rifampicin were 78.0%, 70.7%, 34.1%, 17.1%, 9.8%, and 2.4%, respectively. All the MRSA strains were sensitive to linezolid, tigecycline, and vancomycin. The resistance rates to quinolones and gentamycin in the CA-MRSA group (both 0%) were lower than those in the HA-MRSA group.
Conclusion
Emergence of MRSA in DFU was associated with a prolonged wound duration and increased consumption of medical resources. CA-MRSA strains predominated among MRSA isolates from diabetic foot wounds and caused more severe infections.
Spatial Estimation of Daily Precipitation in Thailand based on Infrared Satellite Images using Artificial Neural Networks
Authors: Inkeaw Papangkorn , Wongsaijai Ben , Poochinapan Kanyuta , Oonariya Chalump , Chaijaruwanich Jeerayut 
Published: March 2023

Abstract
Precipitation data highly benefits water management and flood warning. In Thailand, an agricultural country, the number of rain gauge stations is relatively small compared to the country’s area. To deal with the problem, many spatial interpolation methods have been adopted. They utilized only geological data from stations with known rainfall values to estimate values at other locations. Characteristics of clouds captured in infrared satellite images could be used to infer rainfall. In this study, infrared satellite satellite images from every 30 minutes were integrated with geological data to spatially estimate daily precipitation. Under different assumptions, we proposed three estimation models, namely AveragedIR-ANN, IRs-ANN, and LocalIRs-CNN. All three models used artificial neural networks as the estimator. The AveragedIR-ANN model used the average of 48 black body temperature (TBB) values to present IR images at the target location as an input. Meanwhile, the 48 TBB values were directly fed into the IRs-ANN. Not only the TBB values at the target location, but the LocalIRs-CNN also used the TBB values around the target location as inputs. Experiments were performed on 24 days per year between 2016 and 2019 with multiple tries. The results show that the LocalIRs-CNN outperformed by minimizing the median highest root mean square error. The model was suggested for northern, western and central Thailand.
Management of encounters related to subfertility and infertility among Aboriginal and Torres Strait Islander females in Australian general practice
Authors: Gilbert Emily , Rumbold Alice , Campbell Sandra , Boyle Jacqueline , Grzeskowiak Luke 
Published: March 2023

Abstract
Objective: To investigate the management of subfertility and infertility among Aboriginal and Torres Strait Islander females attending Australian general practice.
Methods: Cross-sectional study of 1,258,581 women (18-49 years) attending general practice between January 2011 and June 2019, utilising data from NPS MedicineWise MedicineInsight, a national general practice database in Australia.
Results: The prevalence of subfertility/infertility encounters was lower for Aboriginal and Torres Strait Islander females (12.4 per 1000) than for non-Indigenous females (16.6 per 1000). Aboriginal and Torres Strait Islander females with a subfertility/infertility encounter were younger and more likely to live outside Major cities and in areas of socioeconomic disadvantage than non-Indigenous females. Rates of prescribed infertility medications were not different between groups, however Aboriginal and Torres Strait Islander females were more likely to receive a pelvic ultrasound (24.3% vs. 19.9%); tests for LH (31.9% vs. 25.7%), testosterone (14.9% vs. 10.0%) and HbA1c (6.3% vs. 3.4%); but less likely to receive an AMH test (2.8% vs. 7.0%).
Conclusions: Lower encounter rates for infertility/subfertility among Aboriginal and Torres Strait Islander people may indicate access issues, preferred use of Aboriginal community-controlled health centres or younger average age at first birth and thus less age-related infertility.
Implications for public health: Future efforts should focus on maximising the inclusiveness of infertility surveillance. There is also a needfor further research into the experiences of and preferences for infertility care and associated barriers among Aboriginal and Torres Strait Islander people.
Oxidative stress increases in liver of lactating rats after BPF-low-dose exposure: perinatal effects in the offspring
Authors: Linillos-Pradillo Beatriz , Rancan Lisa , Murias Julio García , Schlumpf Margret , Lichtensteiger Walter , Tresguerres J. A.F. , Vara Elena , Paredes Sergio D. 
Published: March 2023

Abstract
Bisphenol F (BPF) is replacing Bisphenol A (BPA) in the manufacture of products due to endocrine-disrupting effects. BPF monomers can also be released into the environment and enter the food chain, resulting in human exposure to low doses. Since bisphenols are primarily metabolized by the liver, this organ is more vulnerable to lower doses of bisphenols than others. Exposure during prenatal development may increase the risk of diseases in adulthood. The aim was to evaluate whether BPF administration could generate oxidative stress in liver of lactating rats, and whether these effects may be also observed in female and male postnatal day 6 (PND6) offspring. Long Evans rats received oral treatment: Control, BPF-low-dose (LBPF) 0.0365 mg/kg b.w./day, and BPF-high-dose (HBPF) 3.65 mg/kg b.w./day. The levels of antioxidant enzymes (CAT, SOD, GR, GPx and GST), glutathione system (GSH, GSSG) and lipid damage markers (MDA, LPO) were measured using colorimetric methods in liver of both lactating dams and in PND6 offspring. Mean values were analyzed using Prism-7. LBPF affected liver defense mechanisms (antioxidant enzymes and glutathione system), increasing ROS levels and producing lipid peroxidation in lactating dams. Similar effects were found in female and male PND6 offspring as a consequence of perinatal exposure.
The Yinota-O-trial: Efficacy of an online yoga-intervention in high-grade glioma patients and their caregivers – patient-reported outcomes and serum stress parameters - a study protocol of a randomized controlled trial
Authors: Rabe Antonia , Keßler Almuth Friederike , Hagemann Carsten , Jörg Schubert , Elisabeth Jentschke 
Published: March 2023

Abstract
Background: High-grade glioma patients and their caregivers often suffer from distress and a lower quality of life. Results from studies with patients with mixed cancer entities suggest that yoga can be an effective support. However, it is unclear whether this also applies to high-grade glioma patients and their caregivers. This study aims to investigate the effects of mindfulness-based online yoga for patients and their caregivers on emotional distress, quality of life and stress-associated physiological parameters compared to a waiting control group (WCG).
Methods: The study is designed as a multicenter randomized controlled trial. Adult glioma patients (CNS WHO grades 3 & 4) and their caregivers will be recruited. Examined yoga instructors deliver the intervention (1 h/week) in a synchronous format over 8 weeks via video conferencing. The WCG will receive standard care during the 8-week waiting period. Data will be collected before and after the end of the intervention and another 3 months later using questionnaires as well as blood serum and hair samples to evaluate biochemical stress parameters. Primary outcome is self-reported generalized anxiety and secondary outcomes are self-reported progression anxiety, depression, quality of life as well as BDNF, DHEA/DHEAS, Ferritin and hair cortisol. We hypothesize better outcomes in the intervention group (IG) compared to the WCG at all measurement points. 70 patients and 70 caregivers will be recruited consecutively. Primary endpoints are significant effect detections in the Generalized Anxiety Disorder Scale-7 of patients and caregivers at the end of the intervention. Analyses of covariance will be performed to analyze treatment effects.
Discussion: This is the first study to analyze the effectiveness of mindfulness-based online yoga for high-grade glioma patients and their caregivers with well-defined inclusion criteria. The consideration of biochemical stress markers will objectively increase the validity of self-reports in addition to supporting results of previous studies on yoga for cancer patients. If online yoga therapy proves effective, it could make yoga classes more attractive compared to face-to-face classes, as glioma patients are often limited in their mobility and caregivers have less time due to caregiving.
Trail registration: German Clinical Trial Register (Nr. DRKS00029554, 08/2022).
Community and Provider Perceptions and Experiences of Cervical Cancer Screening in Rural Bolivia: A Qualitative Study
Authors: Basagoitia Armando , Burrowes Sahai , Solis Maria Teresa , MacMillan Genevieve , Sullivan Sarah 
Published: March 2023

Abstract
Background: Despite efforts to increase cervical cancer screening access in rural Bolivia, uptake remains low. Bolivia has one of the highest cervical cancer mortality rates in the Americas. As it redoubles efforts to deliver universal health care, the Bolivian government needs information on the factors constraining cervical cancer screening access, and utilization, especially in rural areas.
Methods: Our qualitative study explored cervical cancer screening barriers and described community and provider perceptions and experiences of care. Bolivian and US researchers analyzed data collected from eight focus groups with male and female community members (n=80) and interviews with healthcare providers (n=6) in four purposively selected rural communities in Monteagudo, Bolivia. Deductive and inductive codes were used to thematically analyze data using MaxQDA software.
Results: Four themes emerged from the data: lack of knowledge/misconceptions, health system inadequacy, lack of confidence in providers, and opportunities for improvement. Both men and women displayed misconceptions about the causes of cervical cancer, its consequences, the recommended screening frequency, and the means of accessing care. Providers noted community members’ lack of knowledge and low risk-perception as utilization barriers but also highlighted poor health service quality and inconsistent health education as factors. Poor healthcare quality was a significant barrier; this included poor patient-provider communication, lack of transportation to screening facilities, and severe delays in receiving test results. Providers also noted problems with provider training and physical space for screening. Community members reported low confidence in nurses to perform screening, preferring doctors and specialists. They also expressed discomfort in having male healthcare providers conduct screening. Providers reported low confidence in government officials to support cervical cancer screening improvements. Suggestions for improvements included more intensive cervical cancer outreach to rural areas, mobile screening, and having specialists train lower-level providers to perform screening.
Conclusions: Our findings suggest that poor healthcare quality has affected screening uptake in addition to physical barriers to care. They indicate a need for initiatives to reduce reporting time for Pap test results, the incorporation of community-based HPV self-testing into screening protocols, and the implementation of programs to improve community confidence in providers’ ability to perform screening.
Establishing a Nomogram to Predict the Risk of Pulmonary Embolism in Oncology Patients
Authors: Liuyi Qiu , Tenggao Chen , Yifang Lu , Wenchen Li , Jianping Chen , Xu Ma 
Published: March 2023

Abstract
Objective
Pulmonary embolism (PE) is a serious disease that can result in high morbidity and mortality among cancer patients. The aim of this study was to create a nomogram to accurately predict PE risk in oncology patients to enhance their medical treatment and management.
Methods
This study was designed as a retrospective analysis; information on medical history, complications, specific clinical characteristics, and laboratory biomarker results was collected for suspected PE patients admitted to the oncology department at the Affiliated Dongyang Hospital of Wenzhou Medical University between January 2012 and December 2021. A total of 512 patients were randomly divided into training and validation groups based on a 6:4 ratio. LASSO and multivariate logistic regressions were used to develop a nomogram-based scoring model. Model performance was evaluated using receiver operating characteristic (AUC), calibration, and clinical decision curves.
Results
In our study, over 50 features from 512 patients were analyzed. The nomogram-based scoring model was established using five predictive features, including the neutrophil count, sex, systolic blood pressure, surgical status, and D-dimer levels, which achieved AUC values of 0.758 and 0.702 in the training (95% CI 0.695–0.804) and validation cohorts (95% CI 0.630–0.776), respectively. For our model, the sensitivity was 85.58%, the specificity was 35.78%, the positive predictive value was 72.44%, and the negative predictive value was 55.71%. The calibration curve results showed a strong consistency between the probability predicted by the nomogram and the actual probability. Decision curve analysis (DCA) also demonstrated that the nomogram-based scoring model produced a favorable net clinical benefit.
Conclusions
In this study, we successfully developed a novel numerical model that can predict PE risk in oncology patients, enabling appropriate selection of PE prevention strategies and reducing unnecessary computed tomography pulmonary angiography (CTPA) scans and their associated adverse effects.
Functional traits of fine roots are primary determinants of richness and composition of microbial communities in subtropical forest soil
Authors: Pan Jinwen , Wu Huili , Xiang Wenhua , Ouyang Shuai , Chen Liang , Zeng Yelin , Deng Xiangwen , Zhao Zhonghui , Zeng Weixian , Kuzyakov Yakov 
Published: March 2023

Abstract
Aims Although plant–microbial interactions are essential in sustaining terrestrial ecosystem functions, root–microbial interactions remain poorly understood. Therefore, effects of fine root species richness, composition and biomass, and functional traits on soil microbial community richness and composition were assessed in subtropical secondary forests.
Methods Fine root identity (DNA meta bar coding) was linked with microbial species (high-throughput Illumina sequencing) to reveal root effects on richness and composition of bacterial and fungal communities.
Results The results showed that bacterial and fungal richness was principally determined by functional dispersion of specific root length (FDis_SRL) and community weighted means of root diameter (CWM_diam), and their richness increased with these two root parameters. Microbial community composition was mainly regulated by FDis_SRL, and the abundance of eutrophic bacteria (r-strategists, e.g., Proteobacteria and Actinobacteria), saprotrophs and pathogens increased with FDis_SRL. These two root parameters still directly affect microbial community richness and composition even after accounting for soil and aboveground plant properties and also indirectly by increasing pH, total nitrogen, TP, and AP and decreasing C:N ratio. Furthermore, the richness and composition of fungi were more affected by fine root traits than bacteria, whereas bacteria were more strongly influenced by soil properties.
Conclusions Overall, soil microbial community richness and composition in subtropical secondary forests were primarily determined by fine root functional traits, especially FDis_SRL and CWM_diam. These results will improve our understanding of the important role of root–microbial interactions in terrestrial biogeochemical processes.
Novel antigenic proteins of Mycoplasma synoviae as potential vaccine candidates
Authors: Shi Huoying , Zhang Guihua , Han Lejiabao , Li Zewei , Chen Yifei , Li Quan , Wang Shifeng 
Published: March 2023

Abstract
Mycoplasma synoviae (M. synoviae) is a serious avian pathogen causing severe economic losses to chicken and turkey producers worldwide. The currently available live attenuated vaccine and inactivated vaccine on the market elicit only limited protection. The aim of this study was to identify antigenic proteins of M. synoviae as potential subunit vaccine candidates. In immunoproteomics and reverse vaccinology analyses, a total 24 candidate antigens were picked out. Five candidate antigens were selected to evaluate immunogenicity and preliminary protection in a chicken model, including RS01790 (lipoprotein, putative sugar ABC transporter), BMP (BMP family ABC transporter substrate-binding protein), GrpE (nucleotide exchange factor), RS00900 (putative nuclease) and RS00275 (Uncharacterized protein). The results showed that the five antigens had good immunogenicity. They could be localized on the M. synoviae cell membrane and adhere to DF-1 cells using indirect immunofluorescence assay. They induced specific humoral and cellular immune responses. Vaccine efficacy was evaluated by observing weight gain, pathogen load, pathological changes. As a result, the vaccinated chickens revealed significantly higher body weight gain, with lower air sac lesion scores and tracheal mucosal thicknesses. The vaccinated chickens also showed lower M. synoviae loads in throat swabs than nonvaccinated chickens. The protective effect of BMP, GrpE and RS00900 vaccines is better than that of RS01790 and RS00275. In conclusion, our study demonstrates the potential of using subunit vaccine, providing new ideas for the development of M. synoviae vaccines. The vaccine candidates may contribute to the future development of therapeutic strategies to control the spread of M. synoviae worldwide.
Bioinspired bi-phasic 3D nano-flowers of MgO/Mg(OH) 2 coated melamine sponge as novel bactericidal agent
Authors: Agarwal Ashutosh , Senevirathna Hasanthi L. , Hwee Koo Seok , Wong Crystal Shie Lyeen , Lim Terence , Cheong Ng Foo , Anariba Franklin , Wu Ping 
Published: March 2023

Abstract
By roughly mimicking the surface architectural design of dragonfly wings, novel bi-phasic 3D nano-flowers of MgO/Mg(OH)2 were successfully synthesized via electrospinning technique. The 3D nano flowers were coated over commercial melamine sponge and extensively characterized by SEM, XRD, FTIR and EDS. The formation of distinct dense 3D nano petals was revealed by SEM images whereby the mean petal thickness and mean distance between the adjacent petals were found to be 36 nm and 121 nm, respectively. The bactericidal activities of synthesized 3D nano-flowers coated melamine sponges were assessed against five different bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa). This study demonstrated significant bactericidal activity of MgO/Mg(OH)2 3D nano flowers coated MS against both Gram-positive and Gram-negative bacteria. Plausible bactericidal mechanisms include physical stretching, puncture and shearing of bacteria cell walls. This study serves to introduce novel bioinspired biomaterial with capacity to reduce risk associated with pathogenic bacterial infections especially in medical devices.
Fine mapping of two recessive powdery mildew resistance genes from Aegilops tauschii accession CIae8
Authors: Tang Xiong , Dai Fangxiu , Hao Yongli , Chen Yiming , Zhang Jianpeng , Wang Guoqing , Li Xingyue , Peng Xiaojin , Xu Tao , Yuan Chunxia , Sun Li , Xiao Jin , Wang Haiyan , Wang Zongkuan , Wang Xiue 
Published: March 2023

Abstract
Wheat powdery mildew (Pm), one of the worldwide destructive fungal diseases, could cause significant yield loss up to 30%. The identification of new Pm resistance genes will enrich the genetic diversity of wheat breeding for Pm resistance. Aegilops tauschii, the ancestor donor of sub-genome D of hexaploid wheat, is a gene pool of beneficial genes that can be easily transferred into wheat by production of synthetic hexaploid wheat followed by genetic recombination. We assessed the Pm resistance level of 35 Ae. tauschii accessions from different origins. Accession CIae8, exhibiting high resistance was used for gene mapping. Inheritance analysis using F2 and F2:3 populations derived from the cross between CIae8 and a Pm susceptible accession PI574467 revealed the Pm resistance was controlled by two independent recessive genes. Bulked segregate analysis using a 55 K SNP array revealed the polymorphic SNPs were concentrated distributed on 2DS (13.5-20 Mb) and 7DS (4.0-15.5 Mb). The Pm resistance loci were named as pmAet-2DS and pmAet-7DS, respectively. By recombinant screening, we narrowed the pmAet-2DS into a 370-kb interval flanked by markers CINAU-AE7800 (14.89 Mb) and CINAU-AE20 (15.26 Mb), mapped the pmAet-7DS to a 160-kb interval flanked by markers CINAU-AE58 (4.72 Mb) and CINAU-AE25 (4.88 Mb). The molecular markers closely linked with the resistance loci and the produced two synthesized hexaploid wheat (SHW) lines laid the foundation for the cloning of the two resistance loci and for their transferring into common wheat.