bluuebunny/crossref_metadata_2025_split huggingface dataset:
Duplications of human longevity-associated genes across placental mammals
Authors: Huang Zixia , Jiang Chongyi , Gu Jiayun , Uvizl Marek , Power Sarahjane , Douglas Declan , Kacprzyk Joanna 
Published: May 2023

AbstractNatural selection has shaped a wide range of lifespans across mammals, with a few long-lived species showing negligible signs of ageing. Approaches used to elucidate the genetic mechanisms underlying mammalian longevity usually involve phylogenetic selection tests on candidate genes, analyses of differential gene expression between age cohorts or species, and measuring age-related epigenetic changes. However, the link between gene duplication and evolution of mammalian longevity has not been widely investigated. Here, we explored the association between gene duplication and mammalian lifespan by analysing 287 human longevity-associated genes across 37 placental mammals. We estimated that the expansion rate of these genes is eight times higher than their contraction rate across these 37 species. Using phylogenetic approaches, we identified 43 genes whose duplication levels are significantly correlated with longevity quotients (FDR < 0.05). In particular, strong correlation observed for four genes (CREBBP,PIK3R1,HELLS,FOXM1) appears to be driven mainly by their high duplication levels in two ageing extremists, the naked mole rat (Heterocephalus glaber) and the greater mouse-eared bat (Myotis myotis). Further sequence and expression analyses suggest that the genePIK3R1may have undergone a convergent duplication event, whereby the similar region of its coding sequence was independently duplicated multiple times in both of these long-lived species. Collectively, this study identified several candidate genes whose duplications may underlie the extreme longevity in mammals, and highlighted the potential role of gene duplication in the evolution of mammalian long lifespans.
Strong breeding colony fidelity in northern gannets following High Pathogenicity Avian Influenza Virus (HPAIV) outbreak
Authors: Grémillet David , Ponchon Aurore , Provost Pascal , Gamble Amandine , Abed-Zahar Mouna , Bernard Alice , Courbin Nicolas , Delavaud Grégoire , Deniau Armel , Fort Jérôme , Hamer Keith C. , Jeavons Ruth , Lane Jude V. , Langley Liam , Matthiopoulos Jason , Poupart Timothée , Prudor Aurélien , Stephens Nia , Trevail Alice , Wanless Sarah , Votier Stephen C. , Jeglinski Jana W.E. 
Published: May 2023

AbstractHigh pathogenicity avian influenza virus (HPAIV) caused the worst seabird mass-mortalities on record in Europe across 2021-2022. The northern gannet (Morus bassanus) was one of the most affected species, with tens of thousands of casualties in the northeast Atlantic between April-September 2022. Disease outbreaks can drastically modify the movement ecology of animals and diminish spatial consistency, thereby increasing the potential for disease transmission. To detect potential changes in movement behaviour, we GPS-tracked breeding adults following the initial HPAIV outbreak, at three of the largest gannet breeding colonies where major mortality of adults and chicks occurred (Bass Rock, Scotland, UK; Grassholm, Wales, UK; Rouzic island, Brittany, France). Crucially, GPS-tracked birds remained faithful to their breeding sites and did not prospect other breeding colonies. They performed regular foraging trips at sea, similar to their behaviour before the outbreak. Gannet foraging effort was nonetheless lower than in 2019, thus surviving birds may have benefited from reduced intra- and interspecific food competition. Breeding colony fidelity of adult northern gannets following HPAIV mass-mortalities suggests limited long-term capacity to virus spread, which may contrast with the behaviour of adults during the disease outbreak, or with that of younger individuals.
The stage- and sex-specific transcriptome of the human parasiteSchistosoma mansoni
Authors: Buddenborg Sarah K. , Lu Zhigang , Sankaranarayan Geetha , Doyle Stephen R. , Berriman Matthew 
Published: May 2023

AbstractThe flatwormSchistosoma mansoniis an important but neglected pathogen that causes the disease schistosomiasis in millions of people worldwide. The parasite has a complex life cycle, undergoing sexual reproduction in a mammalian host and asexual replication in a snail host. Understanding the molecular mechanisms the parasite uses to transition between hosts and develop into dimorphic reproductively competent adults may reveal new strategies for control. We present the first comprehensive transcriptomic analysis ofS. mansoni, from eggs to juvenile worms. Focusing on eight life stages spanning free-living water-borne and parasitic stages from both intermediate and definitive hosts, we have generated deep RNA-seq data for five replicates per group for a total of 75 data sets. The data were produced using a single approach to increase the accuracy of stage-to-stage comparisons and made accessible via a user-friendly tool to visualise and explore gene expression (https://lifecycle.schisto.xyz/). These data are valuable for understanding the biology and sex-specific development of schistosomes and the interpretation of complementary genomic and functional genetics studies.
Simulation of 69 microbial communities indicates sequencing depth and false positives are major drivers of bias in Prokaryotic metagenome-assembled genome recovery
Authors: Nunes da Rocha Ulisses , Kasmanas Jonas Coelho , Toscan Rodolfo , Sanches Danilo S. , Magnusdottir Stefania , Saraiva Joao Pedro 
Published: May 2023

ABSTRACTWe hypothesize that sample evenness, sequencing depth and taxonomic relatedness influence the recovery of metagenome-assembled genomes (MAGs). To test this hypothesis, we assessed MAG recovery in three in silico microbial communities composed of 42 species with the same richness but different sample evenness, sequencing depth and taxonomic distribution profiles using three different pipelines for MAG recovery.The pipeline developed by Parks and colleagues (8K) generated the highest number of MAGs and the lowest number of true positives per community profile. The pipeline by Karst and colleagues (DT) showed the most accurate results (∼ 92%), outperforming the 8K and Multi-Metagenome pipeline (MM) developed by Albertsen and collaborators. Sequencing depth influenced the accurate recovery of genomes when using the 8K and MM, even with contrasting patterns: the MM pipeline recovered more MAGs found in the original communities when employing sequencing depths up to 60 million reads, whilst the 8K recovered more true positives in communities sequenced above 60 million reads. DT showed the best species recovery from the same genus, even though close-related species have a low recovery rate in all pipelines.Our results highlight that more bins do not translate to the actual community composition and that sequencing depth plays a role in MAG recovery and increased community resolution. Even low MAG recovery error rates can significantly impact biological inferences. Our data indicates the scientific community should their findings from MAG recovery, especially when asserting novel species or metabolic traits.
Role of the inner membrane cytochrome ImcH inGeobacterextracellular electron transfer and energy conservation
Authors: Pimenta Andreia I. , Paquete Catarina M. , Morgado Leonor , Edwards Marcus J. , Clarke Thomas , Salgueiro Carlos A. , Pereira Inês A.C. , Duarte Américo G. 
Published: May 2023

AbstractElectroactive bacteria combine the oxidation of carbon substrates with an extracellular electron transfer (EET) process that discharges electrons to an electron acceptor outside the cell. This process involves electron transfer through consecutive redox proteins that efficiently connect the inner membrane to the cell exterior. In this study, we isolated and characterized the quinone-interacting membrane cytochromecImcH fromGeobacter sulfurreducens, which is involved in the EET process to high redox potential acceptors. Our work provides evidence that ImcH is electroneutral, as it transfers electrons and protons to the same side of the membrane, contributing to the maintenance of a proton motive force, and plays a central role in recycling the menaquinone pool.ImportanceGeobacter sulfurreducensis a model electroactive bacterium, widespread in the environment and of significant interest for biotechnological applications. Its ability to form thick and conductive biofilms on top of conducting surfaces makes this microbe very useful in bioelectrochemical systems for the production of energy or added value products. To exploreGeobacterspp. as a biocatalyst it is essential to understand its metabolism, particularly the molecular mechanisms for extracellular electron transfer and energy conservation. Our results reveal the importance of ImcH in both processes, identifying this protein as a major player onGeobactermetabolism.
Production and characterization of GNPs-reinforced Al-based composite material by mechanical alloying
Authors: Behret Göksel , Sahin Oguzhan , Erturun Veysel 
Published: May 2023

Purpose
The purpose of this study is to investigate the effects of graphene nanoplates (GNPs), and alloying time on aluminum matrix composite. After mixing the powdered materials in the alloying device, solid samples were formed by sintering. It is thought that the effect of using such a mechanical mixer on GNPs will be investigated and this study will give a perspective on the composites that GNPs will make with Al and its alloys.

Design/methodology/approach
Mechanical alloying (MA) device capable of high-speed shaking (Spex) movement was used and alloying was performed by adding other metal powders into aluminum, which is the matrix material, with the addition of GNPs at three different rates and times. The crystal size and lattice stress parameters were calculated by obtaining the X-ray diffraction (XRD) graphics of the obtained powder mixtures.

Findings
The XRD graphs of the obtained powder mixtures showed that the Al peaks decreased when the MA time increased. When the scanning electron microscopy images of the powder mixtures were examined, it was observed that agglomeration occurred especially in 1 and 1.5 Wt.% graphene reinforced mixtures that were MA for 90 min. The increase in the amount of graphene had a negative effect on the homogeneous distribution. When the Vickers microhardness values of the samples were examined, the hardness value of all samples increased up to the MA time of 45 min, and decreased in the times over 45 min.

Originality/value
The use of GNPs in the mechanical alloying technique and the fact that this technique was performed on the Retsch MM 400 device, which is a Spex type mixer, shows the originality of the study in terms of time and material content.
The impacts of supply visibility and demand visibility on product innovation: the mediating role of supply chain integration
Authors: Hu Miao , Jiang Shenyang , Huo Baofeng 
Published: May 2023

PurposeDrawing on absorptive capacity theory, this study explores the impacts of supply visibility and demand visibility on product innovation (i.e. exploratory and exploitative innovation), and it examines how supplier integration, customer integration and internal integration mediate these impacts.Design/methodology/approachThe authors employ empirical survey data from 200 Chinese manufacturers and use structural equation modeling to test the proposed relationships.FindingsThe results show that supply visibility is positively related to supplier integration and internal integration and that demand visibility is positively related to customer integration. Furthermore, only customer integration and internal integration positively relate to exploratory and exploitative innovation.Originality/valueFirst, this study emphasizes that supply visibility and demand visibility are important sources of a firm's innovation performance and that supply chain integration increases focal firms' capability of exploiting information and facilitates product innovation. Second, the study shows that supply visibility and demand visibility have distinct effects on three dimensions of supply chain integration and exploratory and exploitative innovation. The study also provides significant managerial guidelines for effectively leveraging supply chain visibility and integration in the promotion of product innovation.
Do refugee inflows contribute to the host countries’ entrepreneurial rates? A dynamic panel data analysis, 2000–2019
Authors: Noorbakhsh Sajad , Teixeira Aurora A.C. 
Published: May 2023

Purpose
This study aims to estimate the impact of refugee inflows on host countries’ entrepreneurial rates. The refugee crisis led to an increased scientific and public policy interest in the impact of refugee inflows on host countries. One important perspective of such an impact, which is still underexplored, is the impact of refugee inflows on host countries entrepreneurial rates. Given the high number of refugees that flow to some countries, it would be valuable to assess the extent to which such countries are likely to reap the benefits from increasing refugee inflows in terms of (native and non-native) entrepreneurial talent enhancement.

Design/methodology/approach
Resorting to dynamic (two-step system generalized method of moments) panel data estimations, based on 186 countries over the period between 2000 and 2019, this study estimates the impact of refugee inflows on host countries’ entrepreneurial rates, measured by the total early-stage entrepreneurial activity (TEA) rate and the self-employment rate.

Findings
In general, higher refugee inflows are associated with lower host countries’ TEA rates. However, refugee inflows significantly foster self-employment rates of “medium-high” and “high” income host countries and host countries located in Africa. These results suggest that refugee inflows tend to enhance “necessity” related new ventures and/ or new ventures (from native and non-native population) operating in low value-added, low profit sectors.

Originality/value
This study constitutes a novel empirical contribution by providing a macroeconomic, quantitative assessment of the impact of refugee from distinct nationalities on a diverse set of host countries' entrepreneurship rates in the past two decades resorting to dynamic panel data models, which enable to address the heterogeneity of the countries and deal with the endogeneity of the variables of the model.
Impact of artificial intelligence text generators (AITGs) on libraries
Authors: Inamdar Saba 
Published: May 2023

Purpose
The purpose of studying the impact of artificial intelligence text generators (AITGs) on libraries is to examine the effect of AITGs on the library landscape, including the services offered, the resources provided and the roles of library staff.

Design/methodology/approach
The current study examined how AITGs impact libraries. The researcher was able to comprehend the problem by critically analyzing and reviewing the pertinent published works, such as books, journals and articles.

Findings
This study concludes AITGs can assist libraries in streamlining operations, enhancing services and making collections more accessible. It is vital to highlight that AITGs are not intended to dissuade its users from visiting physical libraries or to replace them with virtual ones. Instead, they are a tool that can improve and supplement the services and resources provided by virtual libraries.

Originality/value
The study’s observations add to the corpus of information on AITGs in libraries and help users comprehend their technological foundations. Further empirical research is recommended on the effects of AITGs and their impact on libraries.
Are emergency department nurse practitioners homeless?
Authors: Pankevich Volha , Maclaine Christopher 
Published: May 2023

AbstractThe professional body for nurses working in emergency medicine is the College of Emergency Nursing Australasia. The professional body for doctors working in emergency medicine is the Australasian College for Emergency Medicine (ACEM). There is no professional body for nurse practitioners working in emergency medicine. The clinical role of nurse practitioners in emergency medicine more closely resembles that of doctors rather than nurses. Associate membership in ACEM would recognise their role in treating patients and would enhance their access to relevant continued professional development as has been endorsed by ACEM. It would also facilitate their further integration into the body of those who autonomously treat patients in the ED.
Molecular‐defined clonal evolution in patients with classical myeloproliferative neoplasms
Authors: Hinze Anna , Rinke Jenny , Crodel Carl C. , Möbius Susanne , Schäfer Vivien , Heidel Florian H. , Hochhaus Andreas , Ernst Thomas 
Published: May 2023

AbstractClassical myeloproliferative neoplasms (MPNs) are characterized by distinct clinical phenotypes. The discovery of driver mutations in JAK2, CALR and MPL genes provided new insights into their pathogenesis. Next‐generation sequencing (NGS) identified additional somatic mutations, most frequently in epigenetic modulator genes. In this study, a cohort of 95 MPN patients was genetically characterized using targeted NGS. Clonal hierarchies of detected mutations were subsequently analysed using colony forming progenitor assays derived from single cells to study mutation acquisition. Further, the hierarchy of mutations within distinct cell lineages was evaluated. NGS revealed mutations in three epigenetic modulator genes (TET2, DNMT3A, ASXL1) as most common co‐mutations to the classical driver mutations. JAK2V617F as well as DNMT3A and TET2 mutations were detected as primary events in disease formation and most cases presented with a linear mutation pattern. Mutations appear mostly in the myeloid lineages but can also appear in lymphoid subpopulations. In one case with a double mutant MPL gene, mutations exclusively appeared in the monocyte lineage. Overall, this study confirms the mutational heterogeneity of classical MPNs and highlights the role of JAK2V617F and epigenetic modifier genes as early events in hematologic disease formation.
Supporting living together: Group work on animal rights for children
Authors: Kara Yunus 
Published: May 2023

AbstractWith this group work, which includes activities for animal rights, it is aimed that children can raise awareness about animal rights and interact with animals. The research was carried out as a pretest‐posttest experimental study in order to evaluate the effect of group work on children receiving counselling and training from a Guidance and Research Center in Istanbul. The study group of the research between May 2022 and June 2022 consists of 20 children (10 in the experimental group, 10 in the control group) between the ages of 11–12 and continuing their education in Istanbul. In addition to the Personal Information Form, which included questions such as gender, age, education status, number of siblings, socio‐economic levels, and status of living with pets, the Oney Animal Love Scale and Scale of Behaviour for Animals were administered to the study group as pretest‐posttest. The group work was held on Saturdays at 13:00, consisted of six sessions, and in each session, activities for socialization, animal rights and child–animal interaction were carried out. The collected data were analysed in the SPSS 22.0 package program. At the end of the group work, it was observed that there was a positive significant difference in the total scores of the Oney Animal Love and Scale of Behaviour for Animals of the children in the experimental group.
Preventing acute liver injury via hepatocyte‐targeting nano‐antioxidants
Authors: Yuan Xuejiao , Zhou Yanfeng , Sun Jinli , Wang Shanshan , Hu Xingjie , Li Jiyu , Huang Jing , Chen Nan 
Published: May 2023

AbstractAcute liver injury (ALI) is a severe liver disease that is characterized by sudden and massive hepatocyte necrosis and deterioration of liver functions. Oxidative stress is increasingly recognized as a key factor in the induction and progression of ALI. Scavenging excessive reactive oxygen species (ROS) with antioxidants has become a promising therapeutic option, but intrinsically hepatocyte‐targeting antioxidants with excellent bioavailability and biocompatibility are yet to be developed. Herein, self‐assembling nanoparticles (NPs) composed of amphiphilic polymers are introduced to encapsulate organic Selenium compound L‐Se‐methylselenocysteine (SeMC) and form SeMC NPs, which protect the viabilities and functions of cultured hepatocytes in drug‐ or chemical‐induced acute hepatotoxicity models via efficient ROS removal. After further functionalization with the hepatocyte‐targeting ligand glycyrrhetinic acid (GA), the resultant GA‐SeMC NPs exhibit enhanced hepatocyte uptake and liver accumulation. In mouse models of ALI induced by acetaminophen (APAP) or carbon tetrachloride (CCl4), treatment with GA‐SeMC NPs significantly decrease the levels of hepatic lipid peroxidation, tissue vacuolization and serum liver transaminases, while prominently increase that of endogenous antioxidant enzymes. Our study therefore presents a liver‐targeting drug delivery strategy for the prevention and treatment of hepatic diseases.
Longitudinal assessment of undergraduate dental students: Building evidence for validity
Authors: Dickie Jamie , Sherriff Andrea , McEwan Michael , Bell Aileen , Naudi Kurt 
Published: May 2023

AbstractPurposeTo investigate the content and criterion validity, and reliability of longitudinal clinical assessment of undergraduate dental student clinical competence by determining patterns of clinical performance and comparing them with validated standalone undergraduate examinations.MethodsGroup‐based trajectory models tracking students' clinical performance over time were produced from LIFTUPP© data for three dental student cohorts (2017–19; n = 235) using threshold models based on the Bayesian information criterion. Content validity was investigated using LIFTUPP© performance indicator 4 as the threshold for competence. Criterion validity was investigated using performance indicator 5 to create distinct trajectories of performance before linking and cross‐tabulating trajectory group memberships with a ‘top 20%’ performance in the final Bachelor of Dental Surgery (BDS) examinations. Reliability was calculated using Cronbach's alpha.ResultsThreshold 4 models showed all students followed a single upward trajectory in all three cohorts, showing clear progression in competence over three clinical BDS years. A threshold 5 model produced two distinct trajectories, and in each cohort a ‘better performing’ trajectory was identified. Students allocated to the ‘better performing’ trajectories scored higher on average in the final examinations for cohort 2 (29% vs 18% (BDS4); 33% vs. 15% (BDS5)) and cohort 3 (19% vs. 16% (BDS4); 21% vs. 16% (BDS5)). Reliability for the undergraduate examinations was high for all three cohorts (≥0.8815) and did not change appreciably when longitudinal assessment was included.ConclusionsThere is some evidence to support that longitudinal data have a degree of content and criterion validity for assessing the development of clinical competence in undergraduate dental students, which should increase confidence in decisions based on these data. The findings also provide a good foundation for subsequent research.