bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between Bone Mineral Density and Serum Uric Acid in the Elderly Chinese: Observational and Mendelian Randomization Analyses
Authors: Xie Min , Yuan Chunchun , Huang Chen , Ji Jiangxun , Cai Feihong , Shi Binhao , Wang Jing , Ye Xiulan , Chai Yuan , Pu Wenjun , Xie Xuehua , Wang Yongjun , Jiang Lijuan , Tang Dezhi , Liang Qianqian 
Published: August 2023

AbstractSummaryAs an endogenous antioxidant, serum uric acid (SUA) can promote the proliferation and differentiation of osteoblasts and prevent osteoporosis. We found the higher SUA levels may associate with lower risk of osteoporosis (OP) in postmenopausal women.MethodsA community-based cross-sectional study was designed on the basis of 2156 postmenopausal women and elderly men (≥50 years old) from Kunming and Jinshan, China. the bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DXA).ResultsA total of 1755 participants were recruited. In observational study, a linear association between SUA and BMD existed only in postmenopausal women, and the association was still significant after other confounding factors were adjusted (P<0.05). The binary logistic regression analysis proved that the odds ratio(OR) for OP in SUA quartile 4 was the lowest (Model 1:OR=0.521<1, 95% confidence intervals (CI), 0.358~0.757,P= 0.01). Restricted cubic spline (RCS) concluded no nonlinear relationship of SUA with BMD in both women and men (P>0.05). Moreover, the Mendelian randomization (MR)analysis showed no causal effect of SUA on BMD at different skeletal sites (IVW causal effect -0.02, 95%CI-0.08 to 0.04,P= 0.55, and MR- -Egger causal effect -0.03, 95%CI-0.13 to 0.06,P=0.50).ConclusionsOur study concluded a positive linear relationship between SUA and BMD only in the postmenopausal women, and a higher SUA level could predict a higher BMD and a lower risk of OP. However, we found no casual effect of SUA on BMD.
Structural insights into epitope-paratope interactions of monoclonal antibody targeting CEACAM5-expressing tumors
Authors: Rak Alexey , Kumar Anand , Duffi Francis , Gagnaire Marie , Rapisarda Chiara , Bertrand Thomas 
Published: August 2023

Abstract

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) are overexpressed in some tumor types. The antibody-drug conjugate, tusamitamab ravtansine, specifically recognizes the A3-B3 domains of human CEACAM5 (hCEACAM5). To understand this specificity, we mapped the epitope-paratope interface between the A3-B3 domains of hCEACAM5 (hCEACAM5A3 − B3) and the antigen-binding fragment of tusamitamab (tusa Fab). Hydrogen/deuterium exchange mass spectrometry identified the tusa Fab paratope, which involves heavy chain (HC) residues 101–109 and light chain residues 48–54 and 88–104. Surface plasmon resonance demonstrated that alanine variants of HC residues 96–108 abolish binding to hCEACAM5, suggesting that these residues are critical for the Fab-antigen complex formation. Cryogenic electron microscopy structure of the tusa Fab-hCEACAM5A3 − B3 complex (3.11 Å overall resolution) revealed a discontinuous epitope involving residues in the A3-B3 domains and an N-linked mannose at residue 612. Conformational constraints on the epitope-paratope interface enable tusamitamab to target hCEACAM5A3 − B3 and differentiate it from other CEACAMs.
Slice-aware 5G Network Orchestration Framework Based on Dual-Slice Isolation and Management Strategy (D-SIMS)
Authors: Venkatapathy Sujitha , Sriniva Thiruvenkadam , Lee Oh-Sung , Jo Han-Gue , Ra In-Ho 
Published: August 2023

Abstract
Network slicing is crucial to the 5G architecture because it enables the virtual-ization of network resources into a logical network. Network slices are created, isolated, and managed using software-defined networking (SDN) and network function virtualization (NFV). The virtual network function (VNF) manager must devise strategies for all stages of network slicing to ensure optimal allocation of physical infrastructure (PI) resources to high-acceptance virtual service requests (VSRs). This paper investigates two independent network slicing frameworks named as dual-slice isolation and management strategy (D-SIMS) and recommends the best of the two based on performance measurements. D-SIMS places VNFs for network slicing using self-sustained resource reservation (SSRR) and master-sliced resource reservation (MSRR), with some flexibility for the VNF manager to choose between them based on the degree to which the underlying physical infrastructure has been sliced. The present research work consists of two 1 phases: the first deals with the creation of slices, and the second with determining the most efficient way to distribute resources among them. A deep neural network (DNN) technique is used in the first stage to generate slices for both PI and VSR. Then, in the second stage, we propose D-SIMS for resource allocation, which uses both the fuzzy-PROMETHEE method for node mapping and Dijk-stra’s algorithm for link mapping. During the slice creation phase, the proposed DNN training method’s classification performance is evaluated using accuracy, precision, recall, and F1 score measures. To assess the success of resource allocation , metrics such as acceptance rate and resource effectiveness are used. The performance benefit is investigated under various network conditions and VSRs. Finally, to demonstrate the importance of the proposed work, we compare the simulation results to those in the academic literature.
Burden of Disease Attributable to Ischemic Heart Disease in Colombia (2015 - 2020)
Authors: Nieto Katherine Helena Avila , Ramirez Alexandra Porras , Mendoza Alejandro Rico 
Published: August 2023

Abstract
Introduction: Ischemic heart disease is a worldwide public health problem, since 2000 the largest increase in deaths corresponds to this, which has increased from more than 2 million deaths in 2000 to 8.9 million in 2019.
Objective: To estimate the burden of disease attributable to ischemic heart disease, measured in disability-adjusted life years DALYs in the Colombian inhabitants from 2015 to 2020.
Methodology: This is an exploratory ecological study in which the burden of disease for ischemic heart disease was estimated by reviewing the trend in mortality and morbidity. Incidence rate data from the Individual Service Provision Records (RIPS) of the Social Protection Information System (SISPRO) and specific mortality rates by age, sex, year and department, according to the official source of vital statistics of the DANE, were used. Years of life lost due to premature death (YLL) and years of life lost due to disability (YLD) were estimated to calculate the DALYs, according to Murray's methodology.
Results: During the years from 2015 to 2020, ischemic heart disease caused a loss of 4,703,914 total disability-adjusted life years. A greater contribution of DALYs is observed in all periods in men compared to women. The distribution by age group indicates that from the age of 60 years onwards, the loss of DALYs is progressive. At the national level, a greater loss was observed in the departments of Quindío, Risaralda, Caldas, Tolima, Valle del Cauca, Atlántico, Bogotá, Santander, Huila and Cundinamarca.
Conclusion: The main cause of mortality in Colombia is ischemic heart disease, which is a major contributor to disability in the country. This scenario and the aging inhabitants point to challenges for the health system and decision makers that require effective national policies and immediate interventions to lessen the negative impact on the health and development of Colombians.
Differentiation of Human umbilical cord-derived mesenchymal stem cells to melanocyte, Osteogenic and Neural cells in vitro
Authors: Zhu Chunxiao , Guo Yuan , Liu Le , wang shichao , cao junwei , liu chunxia , Zhou Huanmin , zhang yanru 
Published: August 2023

Abstract
This study aimed to isolated and culture human umbilial cord mesenchymal stem cells (hUMSCs) in vitro by tissue block attachment to investigate their biological characteristics. The hUMSCs were cultured to passage 3, following which the induction experiments were performed in vitro and induced to osteoblasts, neurons, and melanocytes. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the expression of Nanog Homeobox (NANOG), Pou Class 5 Homeobox 1 (OCT4), 5'-nucleotidase ecto (CD73), CD44 molecule (CD44) in umbilical cord-derived mesenchymal stem cells were positive and population doubling time was 24.7 h. The differentiation properties of neurogenesis and osteogenic cells were confirmed by histological staining using toluidine blue and Alizarin red. Melanocytes were detected by RT-PCR, quantitative PCR (qPCR), and immunofluorescence staining. After 28 days of differentiation, the cells exhibited a typical morphology such as bipolar or tripolar cells with slender dendrites. The immunofluorescence staining showed that the differentiated cells expressed microphthalmia transcription factor (MITF) and tyrosinase (TYR), the expression of MITF, TYR and KIT proto-oncogene receptor tyrosine kinase (KIT) from 0d to 28 days by qPCR was significantly different and the expression of marker genes MITF, SOX10, KITby RT-PCR were positive. The results demonstrated that the cells isolated from human umbilical cord were mesenchymal stem cells, and hUMSCs had multidifferentiation potentialities. hUMSCs could also differentiate into melanocytes in vitro, providing reliable sources of melanocytes for treating vitiligo future.
Multi-omics Blueprint of Cellular Senescence in Deciphering Immune Characteristics and Prognosis Stratification of CRC
Authors: Huang He , Wu Jiezhong , Deng Xiaohong , Tu Xusheng , Li Fen , Ke Chunlian 
Published: August 2023

Abstract
Introduction:
As one of the most common digestive system malignancies, colorectal cancer (CRC) imposed grave danger on the public health. Cellular senescence involves gradual changes in functionality and reproducibility leading to abnormalities, including apoptosis resistance and enhanced secretion of inflammatory factors.
Methods
Cellular senescence-related gene set was determined by the application of WCGNA. We performed single-cell annotations of CRC cells and determined crucial signaling pathways through Cell chat analysis. Using LASSO and Cox analyses, we identified a gene set with prognostic values. Our model was validated using independent external cohort. In addition, we employed ssGSEA and xCell analyses to describe the detailed profile of infiltrated immune cells.
Results
We identified 3 distinct cell clusters in CRC samples, including T cells, myeloid cells, and B cells. We found that MIF signaling to CD74 + CD44 and CXCR4 displayed the highest interaction probability in the B cells communication. We determined a set of 6 genes of prognostic significance, GPR88, PTH1R, SFRP2, GPX3, ELFN1, and MS4A2. The prognostic differences between the two groups in the internal and external sets were found to be statistically significant. We observed higher infiltration of the activated B cells, CD4 + T cells, and CD8 + T cells in the LR group, which was characterized with an inferior prognosis. The abundance of CD8 + T cells were highly correlated with plasmacytoid and activated dendritic cells and follicular T helper cells.
Conclusion
Our study developed a prognostic model based on cellular senescence, which demonstrated significant efficacy in stratifying patients with CRC. Our findings offer new insights into potential precision immune treatments for this disease, with the hope of improving patient outcomes.
SFDB-MLPP: A Secure and Fine-Grained Data Sharing Scheme with Blockchain Based on Multi-Level Privacy Protection
Authors: Lin Junyu , Feng Libo , Wang Jinli , Qiu Fei , Yu Bei , Cheng Jing , Yao Shaowen 
Published: August 2023

Abstract
As the network communication and service capabilities rapidly improve in the 5G era, billions of terminal devices achieve global interconnection and intercommunication, which leads to the generation of massive data. However, the existing cloud-based data sharing mechanism faces challenges such as sensitive information leakage and data islands, which makes it difficult to achieve secure sharing across domains. The emerging Blockchain technology can achieve secure transmission among unfamiliar nodes without relying on trusted third parties, which can provide a solution for data sharing in the cloud environment of 5G mobile internet. In this paper, we propose a fine-grained and efficient data sharing scheme based on Blockchain and ciphertext policy attribute-based encryption (CP-ABE), and design a verifiable outsourced computation method to reduce the computational pressure of end users. Secondly, we comprehensively consider the user’s identity privacy and transaction privacy, and propose a multi-level privacy protection method based on ring signature and garbled bloom filter, which enhances the user’s data privacy and availability, and prevents the traceability of requests. Finally, we design a set of interconnected smart contracts, and verify that our scheme can achieve secure and efficient data sharing through security analysis and performance testing.
Optimizing Network Performance and Power Consumption in Green Optical Networking: A Dual Algorithm Approach
Authors: Swamidoss Mathumohan , Samiayya Duraimurugan , Gunasekar Manikandan 
Published: August 2023

Abstract
Nowadays, there is an increasing need for advanced technological solutions due to concerns about the energy crisis and environmental protection. In particular, there has been a growing focus on developing green Information and Communication Technologies (ICT) solutions, as the energy consumption of ICT devices has been rapidly increasing in recent decades. This paper introduces EPSO-based flow-aware routing mechanisms that aim to minimize Queueing Delay, Power Consumption (Energy Dissipation), and Load Deviation. Two flow-aware mechanisms, namely the Flow aware load adaptive scheme (FA-LAR) and the flow-aware distance adaptive scheme (FA-DAR), are utilized in this study. The goal is to avoid traffic congestion across the routes by selecting the most direct and efficient paths for the flows. These routed paths are optimized using the MEPSO-based FA-DAR routing algorithm, resulting in significant energy savings. Extensive numerical simulations are conducted to evaluate the proposed algorithms using metrics such as throughput, routing efficiency, queuing delay, load deviation, and energy efficiency. The simulation results demonstrate that the proposed method outperforms existing benchmark methods in all metrics. Furthermore, it ensures lower power consumption, queuing delay, and load deviation. The method exhibits a 30% improvement in throughput and a 24% improvement in energy efficiency compared to existing benchmark methods.
Identification of Triple-Negative Breast Cancer Subtypes Based on Ferroptosis- Related Gene Signature for Targeted Treatment Prioritization
Authors: Chen Yongzhen , Huang Xiaoying , Wang Haining , Zheng Yanan , Su Zhongping , Ling Tao , Xu Yujie , You Qiang , Ji Jie , Zhao Ting 
Published: August 2023

Abstract
Ferroptosis, an iron-dependent form of programmed cell death, plays a crucial role in cancer therapies. However, its impact on chemotherapy, immune checkpoint inhibitor (ICI) treatments, and molecular subtypes of triple-negative breast cancer (TNBC) is poorly understood. In this study, we utilized the FUSCC TNBC cohort to classify TNBC patients into distinct subtypes based on the expression of eight ferroptosis-related genes (FRGs). We employed Gene Ontology (GO) and Gene Set Variation Analysis (GSVA) to characterize the immune phenotype and enriched pathways associated with these subtypes. Additionally, we developed the FerrScore model to identify potential compounds and predict the benefits of ICIs in TNBC patients. Our analysis revealed two ferroptosis-related subtypes with contrasting overall survival (OS) outcomes. Cluster 1 had superior OS and exhibited a "hot" tumor phenotype with increased immune cell infiltration and elevated expression of immune checkpoints compared to Cluster 2. We identified Everolimus as the most promising candidate drug for TNBC patients with a high FerrScore, considering CMap score, experimental evidence, and clinical trial status. Moreover, we validated FerrScore as a powerful metric for predicting the benefits of various ICIs. These findings highlight the influence of ferroptosis on the tumor microenvironment, enabling the classification of TNBC patients into subgroups with different OS outcomes. The FerrScore model has potential in screening compounds and predicting the benefits of ICIs in TNBC, offering valuable insights for treatment prioritization in clinical settings.
Safety assessment of L-Arg oral intake in healthy subjects: a systematic review of randomized control trials
Authors: Kuramochi Yui , Murata Mai , Sumino Akihide , Sone Hideko , Hayamizu Kohsuke 
Published: August 2023

Abstract

L-Arg (L-Arg) is a nonessential amino acid but has many physiological roles. Accordingly, L-Arg has been used in various fields, but there is only limited information available about its safety upon overdose. Generally, the no observed adverse effect level (NOAEL) is used when setting the upper amount for chemical substances. Recently, systematic reviews have been used to assess the safety as well as the effectiveness and usefulness of them. Therefore, we conducted an assessment of the safety of the oral intake of L-Arg in healthy subjects by using gastrointestinal symptoms as an index. We limited the study design to only double-blind randomized controlled trials and searched PubMed, Cochrane Library, EBSCOhost, and Ichushi-Web from inception until May 2021. Assessment of the quality of studies was conducted using the Cochrane Collaboration tool and Jadad score, and the random effects model was used for data analysis. Ultimately, 34 studies were selected for inclusion in this work. The dosage of L-Arg used in the studies ranged from 2000 to 30,000 mg/day (or / one-time dose), and the treatment duration was 1 to 84 days. The increased risk of gastrointestinal symptoms associated with L-Arg intake from 23 studies (647 participants in total) in which such symptoms were reported was 0.01 (95% confidence interval: -0.02 to 0.04), which was not significant difference. NOAEL was estimated as 7531 mg/ one-time dose by using a weighted change-point regression model. (UMIN000046133)
WITHDRAWN: LINC01770 stabilizes TEAD1 expression to mediates nasopharyngeal cancer radiation resistance rendering tumor cells vulnerable to ferroptosis
Authors: Xu Qing-qing , Wen Xin , Huang Cheng-long , Lin Zai-shan , Xu Zhen , Sun Ci-ming , Li Li , Zhang Sui-xian , Song Shuang-Hong , Lou Jia-hao , Hou Zan , Chen Yuan-yuan , Li Xuan , Chen Lei 
Published: August 2023

Abstract
Background
Long noncoding RNAs (lncRNAs) regulate various essential biological processes, including cell proliferation, differentiation, apoptosis, migration, and invasion. However, in nasopharyngeal carcinoma (NPC), the clinical significance and mechanisms of lncRNAs in malignant progression are unknown.
Methods
LINC01770 expression was determined using quantitative real-time reverse transcription PCR, and its prognostic value was evaluated using Kaplan-Meier survival analysis. RNA sequencing and bioinformatic analysis were used to determine the potential function of LINC01770, and its biological effects were investigated using in vitro and in vivo experiments. Mass spectrometry-coupled RNA pull-down assays and western blotting identified LINC01770 interacting proteins, followed by confirmation using RNA immunoprecipitation (RIP) assays. Ferroptosis and lipid peroxidation were detected using flow cytometry.
Results
LINC01770 was overexpressed in NPC tissues according to microarray screening. Patients with NPC showing high LINC01770 expression experienced shorter survival and worse prognosis. In vitro and in vivo experiments suggested that knockdown of LINC01770 expression significantly inhibited the proliferation, migration, and invasion of NPC cells. Sequencing and functional complementation experiments showed that LINC01770 regulates the proliferation and metastasis of NPC through TEA domain transcription factor 1 (TEAD1). Meanwhile, RIP and PCR experiments suggested that LINC01770 and TEAD1 were common targets of microRNAs miR-615-5p and miR-1293. Overexpression of LINC01770 promoted ferroptosis in vitro and in vivo through the TEAD1/Acyl-CoA synthetase long chain family member 4 (ACSL4)/transferrin receptor (TFRC) pathway.
Conclusions
LINC01770 is a prognostic biomarker for NPC and participates in the regulation of TEAD1 signaling pathway through competitive binding to miRNA-615-5p and miRNA-1293, resulting in NPC metastasis and progression. Radiation resistant cells are in a delicate balance between lipid peroxidation and increased vulnerability to ferroptosis, suggesting that ferroptosis could be used to kill NPC cells and reverse their radiotherapy resistance during the malignant progression of NPC caused by high expression of LINC01770. Thus, inducing ferroptosis could be used to treat recurrent and refractory NPC.
The Role of Water in Radiation-induced Fragmentation of Cellulosic Backbone Polysaccharides
Authors: Muscolino Emanuela , Sabatino Maria Antonietta , Jonsson Mats , Dispenza Clelia 
Published: August 2023

Abstract
Xyloglucan (XG) is a cellulosic backbone polysaccharide commercially used for food applications, but also widely investigated in biomedical applications, for its gelling properties and specific biological activity. In this study, the possibility of using gamma radiation to cleave XG and generate lower molecular weight variants was explored. The impact of absorbed dose and irradiation conditions on the XG molecular weight distribution was investigated. Two other cellulosic polysaccharides, hydroxypropyl cellulose (HPC) and an oxidized variant of XG (CXG), were also studied for comparison. Before irradiation, the polymers were characterized with thermal gravimetric analysis (TGA) and, after irradiation, with gel filtration chromatography (GFC). The results showed that for XG irradiated in dilute aqueous solution, a dose of 10 Gy is sufficient to significantly reduce the polymer molecular weight, while HPC is less affected by irradiation under identical conditions. When the polymers were irradiated in the solid form, either dry or humid, the reduction in average molecular weight is much less pronounced. Interestingly, for HPC the cleavage of the chains is more pronounced for the dry than for the humid powder. A similar behavior, but less pronounced, was observed for XG and CXG. Arguably, when water was present in the system as bound water it had a protective effect. This is probably due to energy transfer from the polymer to the bound water preventing chain scission. Indeed, humid HPC has more bound water than XG and CXG. Conversely, when water was present as solvent, water radiolysis products were able to efficiently induce depolymerization.