bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deposition and process parameter optimization of electroless Ni-B coating from a stabilizer free bath to achieve enhanced microhardness, scratch-hardness and adhesion using taguchi’s methodology
Authors: Karn Anand Kumar , Agrawal Rohit , Kumar Abhinandan , Mukhopadhyay Arkadeb 
Published: August 2023

Abstract
Electroless nickel boron coatings have wide industrial usage. However, they are generally obtained from a lead-stabilized bath. The present work investigates and optimizes the scratch-hardness and microhardness obtained from stabilizer-free electroless nickel boron bath in a quest to eliminate lead nitrate/heavy metals, which are potentially toxic. The bath temperature, heat treatment temperature, and duration were varied at three levels. Enhanced scratch-hardness (12.581 GPa) was obtained at 85 °C bath temperature and heat treatment at 350 °C for 1 h. At the same time, the highest microhardness (886.17 HV100) was obtained at a parametric combination of 95 °C bath temperature and heat treatment at 450 °C for 1 h. Multi-objective optimization was carried out using grey relational analysis. The parametric combination predicted in multi-objective optimization was 85 °C bath temperature and heat treatment at 350 °C for 1 h where the microhardness was 846.34 HV100. Furthermore, an analysis of variance was also carried out to investigate the importance of the factors in controlling scratch-hardness and microhardness. The highest contribution was observed from heat treatment duration. Further investigation of the optimized coating was done by the progressive scratch test, which recorded that the first critical load of failure improved compared to non-heat treated electroless Ni-B coatings. The coatings were also characterized using field emission scanning electron microscope, energy dispersive x-ray spectroscopy, and x-ray diffraction. The coatings in optimized condition showed no transverse or chevron cracks within 5–24 N.
Accumulation of squalene in filamentous fungi Trichoderma virens PS1-7 in the presence of butenafine hydrochloride, squalene epoxidase inhibitor: biosynthesis of 13C-enriched squalene
Authors: Zhang Wen , Sunami Kazu , Liu Shuo , Zhuang Zihan , Sakihama Yasuko , Zhou Da-Yang , Suzuki Takeyuki , Murai Yuta , Hashimoto Makoto , Hashidoko Yasuyuki 
Published: August 2023

ABSTRACT
Squalene is a triterpenoid compound and widely used in various industries such as medicine and cosmetics due to its strong antioxidant and anticancer properties. The purpose of this study is to increase the accumulation of squalene in filamentous fungi using exogeneous butenafine hydrochloride, which is an inhibitor for squalene epoxidase. The detailed settings achieved that the filamentous fungi, Trichoderma virens PS1-7, produced squalene up to 429.93 ± 51.60 mg/L after culturing for 7 days in the medium consisting of potato infusion with glucose at pH 4.0, in the presence of 200 µm butenafine. On the other hand, no squalene accumulation was observed without butenafine. This result indicated that squalene was biosynthesized in the filamentous fungi PS1-7, which can be used as a novel source of squalene. In addition, we successfully obtained highly 13C-enriched squalene by using [U-13C6]-glucose as a carbon source replacing normal glucose.
Enhanced overexpression of secreted enzymes by discrete repeat promoters in Streptomyces lividans
Authors: Yang Lingli , Hatanaka Tadashi 
Published: August 2023

ABSTRACT
Streptomyces lividans is an efficient host for extracellular overproduction of recombinant proteins. To enhance the overexpression strength of S. lividans, we designed several kinds of expression plasmids with different positioning of repeat promoters. The effect of repeat promoters was evaluated by measuring the accumulated amounts of a stable transglutaminase or an unstable carboxypeptidase that was secreted into the medium. Successive tandem positions of repeat promoters upstream of the normal promoter did not enhance the expression of transglutaminase. Discrete positions of repeat promoters both upstream and downstream of the normal promoter enhanced the expression of transglutaminase to 2-fold, and the downstream ones also enhanced the expression of carboxypeptidase to 1.7-fold. On the other hand, there were still some constructs of plasmids with discrete repeat promoters that did not promote the expression of the target enzymes, indicating the complexity of the mechanisms of repeat promoters working on gene expression.
Prediction of pathogenic single amino acid substitutions using molecular fragment descriptors
Authors: Zadorozhny Anton , Smirnov Anton , Filimonov Dmitry , Lagunin Alexey 
Published: August 2023

Abstract

Motivation
Next Generation Sequencing technologies make it possible to detect rare genetic variants in individual patients. Currently, more than a dozen software and web services have been created to predict the pathogenicity of variants related with changing of amino acid residues. Despite considerable efforts in this area, at the moment there is no ideal method to classify pathogenic and harmless variants, and the assessment of the pathogenicity is often contradictory. In this article, we propose to use peptides structural formulas of proteins as an amino acid residues substitutions description, rather than a single-letter code. This allowed us to investigate the effectiveness of chemoinformatics approach to assess the pathogenicity of variants associated with amino acid substitutions.

Results
The structure-activity relationships analysis relying on protein-specific data and atom centric substructural multilevel neighborhoods of atoms (MNA) descriptors of molecular fragments appeared to be suitable for predicting the pathogenic effect of single amino acid variants. MNA-based Naïve Bayes classifier algorithm, ClinVar and humsavar data were used for the creation of structure-activity relationships models for 10 proteins. The performance of the models was compared with 11 different predicting tools: 8 individual (SIFT 4G, Polyphen2 HDIV, MutationAssessor, PROVEAN, FATHMM, MVP, LIST-S2, MutPred) and 3 consensus (M-CAP, MetaSVM, MetaLR). The accuracy of MNA-based method varies for the proteins (AUC: 0.631–0.993; MCC: 0.191–0.891). It was similar for both the results of comparisons with the other individual predictors and third-party protein-specific predictors. For several proteins (BRCA1, BRCA2, COL1A2, and RYR1), the performance of the MNA-based method was outstanding, capable of capturing the pathogenic effect of structural changes in amino acid substitutions.

Availability and implementation
The datasets are available as supplemental data at Bioinformatics online. A python script to convert amino acid and nucleotide sequences from single-letter codes to SD files is available at https://github.com/SmirnygaTotoshka/SequenceToSDF. The authors provide trial licenses for MultiPASS software to interested readers upon request.
Block Aligner: an adaptive SIMD-accelerated aligner for sequences and position-specific scoring matrices
Authors: Liu Daniel , Steinegger Martin 
Published: August 2023

Abstract

Motivation
Efficiently aligning sequences is a fundamental problem in bioinformatics. Many recent algorithms for computing alignments through Smith–Waterman–Gotoh dynamic programming (DP) exploit Single Instruction Multiple Data (SIMD) operations on modern CPUs for speed. However, these advances have largely ignored difficulties associated with efficiently handling complex scoring matrices or large gaps (insertions or deletions).

Results
We propose a new SIMD-accelerated algorithm called Block Aligner for aligning nucleotide and protein sequences against other sequences or position-specific scoring matrices. We introduce a new paradigm that uses blocks in the DP matrix that greedily shift, grow, and shrink. This approach allows regions of the DP matrix to be adaptively computed. Our algorithm reaches over 5–10 times faster than some previous methods while incurring an error rate of less than 3% on protein and long read datasets, despite large gaps and low sequence identities.

Availability and implementation
Our algorithm is implemented for global, local, and X-drop alignments. It is available as a Rust library (with C bindings) at https://github.com/Daniel-Liu-c0deb0t/block-aligner.
Salting-Out Assisted Liquid–Liquid Microextraction and Reverse-Phase Chromatographic Quantification of Two Neonicotinoid Insecticides from Fruits and Vegetables
Authors: Akram Sumia , Sultana Bushra , Aasi Muhammad Rafique , Mushtaq Muhammad , Liu Rui Hai 
Published: August 2023

Abstract
The present monograph describes the salting-out assisted liquid–liquid microextraction (SALLME) and reverse-phase high-performance liquid chromatography with diode array detector (RP-HPLC-DAD) based quantification of two frequently applied neonicotinoid insecticides, i.e., acetamiprid (ACE) and imidacloprid (IMD), from selected tropical fruits (citrus and guava) and vegetables (tomato, okra and cauliflower). The SALLME conditions like pH, liquid/solid ratio and salt ratio (NaCl/Na2SO4) were varied over a wide range of conditions for the enhanced recovery of IMD and ACE from spiked quality control (low, high and medium) of fruits and vegetables. Meanwhile, RP-HPLC-DAD quantification of IMD and ACE was found to be linear over 1–100 μg/L with the coefficient of determination (R2) value ≥0.997 and slope of the calibration curve (sensitivity) ≥2.3 × 104 peak area unit (μAu). The analysis of selected fruits and vegetables after 0 (1 h), 1, 3, 7, 10 and 14 days of foliar application revealed the presence of IMD and ACE residues in okra and guava higher than maximum residual limits set by the Codex Alimentarius Commission until the third day of spray. Overall, the SALLME-RP-HPLC-DAD was found to be a rapid, selective and expedient choice for the routine analysis of neonicotinoids in environmental and food commodities.
Hypertriglyceridemia-Associated Pancreatitis: New Concepts and Potential Mechanisms
Authors: Hansen Signe E J , Varbo Anette , Nordestgaard Børge G , Langsted Anne 
Published: August 2023

Abstract

Background
Triglycerides are a major source of energy, while high plasma triglycerides are a risk factor for various diseases and premature death. Severely elevated plasma triglycerides are a well-established cause of acute pancreatitis with high mortality, likely due to the presence of elevated levels of chylomicrons and large very low-density lipoproteins in plasma. As markedly elevated levels of these very large lipoproteins are not generally found in mild to moderate hypertriglyceridemia, this was previously not regarded as a cause or marker of increased risk of acute pancreatitis. However, mild to moderate hypertriglyceridemia may identify individuals who at a later timepoint develop severe hypertriglyceridemia and acute pancreatitis.

Content
We describe measurement of plasma triglycerides and studies on plasma triglycerides and risk of acute pancreatitis. Further, we summarize current European and American guidelines for the prevention of acute pancreatitis and, finally, the potential for future prevention of acute pancreatitis through lowering of plasma triglycerides.

Summary
Recent observational and genetic studies indicate that mild to moderate hypertriglyceridemia is causally related to increased risk of acute pancreatitis, most likely as a marker of future severe hypertriglyceridemia. Current guidelines do not mention individuals with mild to moderate hypertriglyceridemia, even though newer evidence suggests an unmet medical need. Treatment could include plasma triglyceride-lowering therapy targeting the pathway for lipoprotein lipase as the main triglyceride degrading enzyme in plasma. Angiopoietin-like 3 and apolipoproteinC-III are inhibitors of lipoprotein lipase, and blocking of these 2 inhibitors is showing promising results in relation to marked triglyceride-lowering and could perhaps be used to prevent acute pancreatitis in the future.
Pulmonary valve replacement in a large and tortuous right ventricle outflow tract with a 32 mm Myval valve under local anaesthesia: challenges and technical considerations: a case report
Authors: Houeijeh Ali , Sudre Arnaud , Juthier Francis , Godart François 
Published: August 2023

Abstract

Background
Pulmonary valve replacement in patients with congenital heart diseases and heart failure is challenging.

Case summary
Here, we describe a case of a patient who had surgical fallot repair with chronic heart failure. Investigations found severe biventricular dysfunction and enlargement due to chronic pulmonary regurgitation. The right ventricle outflow tract was tortuous and large with a diameter of 35 mm. Percutaneous pulmonary valve implantation (PPVI) was done after a challenging pre-stenting. A 32 mm Myval valve over-sized to 35 mm was used for PPVI, which yielded a good result.

Discussion
A 32 mm Myval valve is effective at extending the possibilities of PPVI in a large and tortuous right ventricle outflow tract not accessible for the other valves.
Short-term recurrence of takotsubo syndrome with phenotypic variation: a case report
Authors: Saito Takafumi , Natsumeda Makoto , Sakagami Takato , Murakami Tsutomu , Takagi Shunsuke , Ikari Yuji 
Published: August 2023

Abstract

Background
Takotsubo syndrome (TTS) is an acute and usually reversible heart failure syndrome characterized as an uncommon left ventricular (LV) cardiomyopathy. Recurrence of TTS is rare, estimated to be 1–6%. We report a rare case of TTS that occurred three times in 2 months but manifested various phenotypes.

Case summary
A 68-year-old woman was admitted to our hospital with acute-onset chest pain and hypertension. The coronary angiography findings were normal, although left ventriculography revealed inferior wall hypokinesis, leading to a mid-ventricular TTS diagnosis. She was discharged on Day 3 after her symptoms improved and vitals stabilized. The patient’s condition remained uneventful until 2-week post-discharge, when acute chest pain and hypertension recurred. She was admitted again with the same diagnosis. However, LV morphology revealed an apical ballooning pattern, with inferior LV wall hypokinesis. She was discharged on Day 7 after her symptoms and electrocardiography findings improved but was readmitted again 2 weeks later after acute chest pain and hypertension recurred. Left ventriculography performed a third time demonstrated mid-ventricular TTS. The patient was prescribed additional medications and discharged on Day 12. Her electrocardiography findings normalized, and the patient remained asymptomatic without recurrence 4 months after the initial presentation.

Discussion
Recurrence and phenotypic change of TTS are rare. Some cases have been reported but occurring months to years after initial diagnosis. Combined treatment with β-blockers and angiotensin-converting enzyme inhibitors or angiotensin receptor antagonists may be more effective to prevent the recurrence than monotherapies.
Faction, Connection and Politics in the Civil Wars: Pembrokeshire, 1640–1649
Authors: Bowen Lloyd 
Published: August 2023

Abstract
This article considers the roles of faction and connections between centre and periphery as structuring elements of local politics during the civil wars of the mid-seventeenth century. It explores these issues through an examination of Pembrokeshire in south-west Wales. Using a considerable amount of previously unexamined printed and manuscript material, it argues that established but largely ignored factional alignments informed a good deal of the political manoeuvring in the county during and after the first civil war, and that these connected to and were complicated by evolving associations with rival political coalitions at Westminster. The article pays close attention to the connections between figures among the Pembrokeshire gentry and MPs and power-brokers in Westminster. It argues that establishing fruitful links with the political centre was vital to the success of an Independent group in the county following the war’s cessation and, conversely, the isolation and ultimate defeat of the Presbyterian party. The article throws new light on the origins of John Poyer’s 1648 rebellion, which initiated the ‘Second Civil War’. It rejects the established narrative which characterises Poyer’s revolt as borne from apolitical self-interest, arguing instead that it was the final act in a factional struggle that was at least a decade old. The piece contends that gentry faction has largely been written out of recent civil war history, but that scholars should be more attentive to its potential for influencing (although not conditioning) political and ideological alignments in the provinces.
Assessing the shooting distance of lead-free ammunition regardless of composition using Laser Induced Breakdown Spectroscopy
Authors: Doña-Fernández Alicia , Rodriguez-Pascual Jose Antonio , de Andres-Gimeno Israel , Gutiérrez-Redomero Esperanza , Valtuille-Fernández Eduardo , Gomez-Laina Francisco Javier 
Published: August 2023

Abstract At present, it is challenging to accurately determine firearm shooting distances in the case that lead-free ammunition is involved, largely because different manufacturers use different primer compositions. Laser-induced breakdown spectroscopy (LIBS) allows the simultaneous detection of multiple elements with high sensitivity and so may represent a solution to this problem. Previous studies have, in fact, demonstrated that LIBS can be used to determine shooting distances when working with gunshot residues from conventional ammunition based on scanning fabric surfaces. The present study confirms that the shooting distance can be ascertained using LIBS to detect copper originating from the ammunition casing and projectile but not the primer on fabric surfaces. This estimation can be performed regardless of the primer composition of lead-free ammunition.Key points
Efficacy and Safety of Dapagliflozin in Patients With Chronic Kidney Disease Across the Spectrum of Frailty
Authors: Vart Priya , Butt Jawad H , Jongs Niels , Schechter Meir , Chertow Glenn M , Wheeler David C , Pecoits-Filho Roberto , Langkilde Anna Maria , Correa-Rotter Ricardo , Rossing Peter , McMurray John J V , Heerspink Hiddo J L 
Published: August 2023

Abstract

Background
A sizeable proportion of patients with chronic kidney disease (CKD) are reported to be frail. Here we examined the safety and efficacy of dapagliflozin in patients with CKD by frailty level.

Methods
Adults with CKD, with/without type 2 diabetes, with an estimated glomerular filtration rate (eGFR) of 25–75 mL/min/1.73 m2, and urinary albumin-to-creatinine ratio 200–5 000 mg/g were randomized to dapagliflozin (10 mg/day) or placebo. The primary endpoint was a composite of sustained ≥50% eGFR decline, end-stage kidney disease (ESKD), or death from kidney or cardiovascular (CV) causes.

Results
Frailty index (FI), assessed by Rockwood cumulative deficit approach, was calculable in 4 303/4 304 (99.9%) patients: 1 162 (27.0%) in not-to-mildly frail (FI ≤0.210), 1 642 (38.2%) in moderately frail (FI 0.211–0.310), and 1 499 (34.8%) in severely frail categories (FI &gt;0.311). Dapagliflozin reduced the risk of the primary composite endpoint across all FI categories (hazard ratios [95% confidence interval {CI}]: 0.50 [0.33–0.76], 0.62 [0.45–0.85], and 0.64 [0.49–-0.83], respectively; p-interaction = 0.67). Results were similar for secondary outcomes including kidney composite outcome (sustained ≥50% eGFR decline, ESKD or death from kidney cause; p-interaction = 0.44), CV endpoint (heart failure hospitalization or CV death; p-interaction = 0.63), and all-cause mortality (p-interaction p = .42). Results were consistent when using FI as a continuous variable. Occurrence of serious adverse events was numerically lower in patients receiving dapagliflozin versus placebo in all FI categories (16.9% vs 20.1%, 26.3% vs 30.7%, and 42.9% vs 47.8%, in not-to-mildly, moderately, and severely frail categories, respectively).

Conclusions
The relative benefit of dapagliflozin for all outcomes was consistent across all frailty categories, with no difference in associated safety.
A Genealogy of theLife History Album(1884): Gerontology, Genre, and Health Across the Life Span
Authors: Bailey Suzanne 
Published: August 2023

AbstractThis paper demonstrates what emerges when we undertake a literary reading of a medical text, examining its form and structure as a text. The study of what appears to be a singular publication reveals instead an under-examined moment in medical history that anticipates contemporary health investigations in modern medicine, while reflecting the limitations of medical and gerontological knowledge in the 1880s. I demonstrate this argument by conducting a Foucauldian archeology of the text, with attention to authorship and the concept of textual genre. My primary text is the Life History Album (1884), which I link to a related endeavor, G. M. Humphry’s Old Age (1889), a little-known publication that contains medical observations on the resiliency of aging bodies and anticipates ideas associated with early twentieth-century geriatrics. My investigation brings new attention to the work of Dr. Frederick Akbar Mahomed, a pioneer in the study of hypertension, whose story is part of the genealogy of the text. Inviting its creator to keep records of health throughout the life span, the Life History Album anticipates a new kind of modern subject, who participates in co-creating his or her medical and life health history, whereas Humphry’s Old Age, which draws on similar methods, is humanistic, includes literary references, and allows for contentment in older age.
Can ChatGPT pass Glycobiology?
Authors: Williams Devin Ormsby , Fadda Elisa 
Published: August 2023

Abstract
The release of text-generating applications based on interactive Large Language Models (LLMs) in late 2022 triggered an unprecedented and ever-growing interest worldwide. The almost instantaneous success of LLMs stimulated lively discussions in public media and in academic fora alike not only on the value and potentials of such tools in all areas of knowledge and information acquisition and distribution but also on the dangers posed by their uncontrolled and indiscriminate use. This conversation is now particularly active in the higher education sector, where LLMs are seen as a potential threat to academic integrity at all levels, from facilitating cheating by students in assignments to plagiarizing academic writing in the case of researchers and administrators. Within this framework, we are interested in testing the boundaries of the LLM ChatGPT (www.openai.com) in areas of our scientific interest and expertise and in analyzing the results from different perspectives, i.e. of a final year BSc student, of a research scientist, and of a lecturer in higher education. To this end, in this paper, we present and discuss a systematic evaluation on how ChatGPT addresses progressively complex scientific writing tasks and exam-type questions in Carbohydrate Chemistry and Glycobiology. The results of this project allowed us to gain insight on: (i) the strengths and limitations of the ChatGPT model to provide relevant and (most importantly) correct scientific information, (ii) the format(s) and complexity of the query required to obtain the desired output, and (iii) strategies to integrate LLMs in teaching and learning.