bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemically controlled blinking of fluorophores to enable quantitative stochastic optical reconstruction microscopy (STORM) imaging
Authors: Gooding John , Yang Ying , Ma Yuanqing , Tilley Richard , Gaus Katharina 
Published: September 2023

Abstract
Stochastic optical reconstruction microscopy (STORM) allows widefield imaging with single molecule resolution through calculating the coordinates of individual fluorophores from the separation of the fluorophore emission in both time and space. Such separation is achieved by photoswitching the fluorophores between a long lived OFF state and an emissive ON state. Despite STORM has revolutionizing cellular imaging, molecular counting in complexes remains challenging due to undercounting errors from photobleached or not-recovered dyes and overcounting artifacts from the repetitive and random blinking of the dyes. Herein we show how an electrochemical approach switching fluorophores for STORM (EC-STORM) has greater control over the switching kinetics, emitter density, and recovery yield than possible photochemically. Using EC-STORM, we demonstrate the capability for molecular counting by applying a programmable electrochemical potential to interrupt the photophysics of dyes. That is, the random blinking of dyes is suppressed by a negative potential but the switching ON event can be activated by a short pulsed positive potential, such that the frequency of ON events scales linearly with the number of underlying dyes. This advance will enable EC-STORM being the widely applicable super resolution imaging technique.
Фолликулярная лимфома 1–3А цитологического типа с наличием или отсутствием t(14;18)(q32;q21): прогноз, выбор противоопухолевой терапии и ее результаты
Authors: Нестерова Екатерина Сергеевна , Звонков E. E. , Ковригина А. М. , Обухова Т. Н. , Бидерман Б. В. , Судариков А. Б. , Мангасарова Я. К. , Магомедова А. У. , Смольянинова А. К. , Куликов С. М. , Паровичникова Е. Н. 
Published: September 2023

Aim. To determine the prognostic value of t(14;18)(q32;q21) in follicular lymphoma (FL) of grades 1–3А, to assess the chemotherapy efficacy in “t(14;18)+ FL” and “t(14;18)– FL” patients, and to analyze the cases of ineffective therapy.
Materials & Methods. The retrospective/prospective study carried out at the National Research Center for Hematology in the period of 2001–2022 enrolled 362 patients with newly diagnosed FL of grades 1–3А. Their risk stratification was based on predictive models FLIPI and PPI3 (Personalized Predictive Index[1]). The patients were 30–81 years of age (median 52 years). There were 225 women and 137 men. They received the following regimens: R-B (n = 80), R-CHOP (n = 189), R-CHOP (4 cycles) + R-DHAP (2 cycles) (n = 28), and R-CHOP (4 cycles) + R-DHAP (2 cycles) + auto-HSCT in the first-line therapy (n = 65). For 2 years, maintenance rituximab therapy was administered to all the enrolled patients, whichever drug chemotherapy they received. Standard cytogenetic analysis and FISH were carried out in 265/362 (73 %) patients.
Results. Patients were divided into two comparable groups: “t(14;18)+ FL” (n = 196) and “t(14;18)– FL” (n = 69). Patients without cytogenetics/FISH (n = 97) were excluded from the analysis. In patients without t(14;18), poor prognostic factors, such as grade 3А (p = 0.003) and Ki-67 > 35 % (p = 0.001), were identified significantly more often, and also high PPI3 risk was reported (p = 0.008). No differences (p = 0.84) were detected during FLIPI risk stratification of patients. Bone marrow lesions were observed significantly more often in “t(14;18)+ FL” compared to “t(14;18)– FL” (p = 0.002). The chemotherapy outcomes, such as 2-year EFS and OS, appeared to be considerably worse in “t(14;18)– FL” compared to “t(14;18)+ FL” patients.
Conclusion. The group of FL patients with t(14;18) appeared to be most numerous and more prognostically favorable. Immunochemotherapy regimens R-B and R-CHOP are more justified in the first-line therapy of FL with low PPI3 risk. Therapy outcomes were comparable in efficacy. In intermediate and high PPI3 risk FL patients with t(14;18), the most effective first-line therapy was the one with consistent administration of R-CHOP, R-DHAP, and auto-HSCT. Based on the results of this study, FL of grades 1–3А without t(14;18) can well be considered to be a prognostically unfavorable variant of this malignant lymphoid tumor. The rate of early relapses/progression after the standard immunochemotherapy (R-B and R-CHOP), according to our data, is 60 %. In patients with newly diagnosed FL who received consistent administration of R-CHOP, R-DHAP, and auto-HSCT in the first-line therapy, this rate drops to 30 %. Our results clearly indicate the need for new FL treatment approaches.
Эндоскопическая семиотика гастритоподобной формы первичных неходжкинских лимфом желудка
Authors: Лозовая Валерия Витальевна , Малихова О. А. , Туманян А. О. 
Published: September 2023

Aim. To characterize the main differentially significant endoscopic features specific to the gastritis-like form of primary gastric non-Hodgkin lymphomas (NHL).
Materials & Methods. This prospective study analyzes the results of complex endoscopic diagnosis in 43 patients with primary gastric NHL. They were examined and treated at the NN Blokhin National Medical Cancer Research Center from 2019 to 2023. The patients were 30–70 years of age, those over the age of 50 predominated and accounted for 79 % (n = 34). There were 33 women and 10 men. The control group included 45 patients with gastritis-like malignant gastric tumors: adenocarcinoma and signet-ring cell cancer.
Results. The morphological analysis yielded a diagnosis of MALT-lymphoma in 90.7 % (n = 39) and diffuse large B-cell lymphoma in 9.3 % (n = 4) of cases. Sensitivity, specificity, and accuracy of the clarification methods of complex endoscopic diagnosis were considerably higher compared to white-light mode examination. According to the results of complex endoscopic analysis, all patients were stratified into 4 groups with different types of H. pylori-induced atrophic gastritis (n = 10; 23.25 %), erosive gastritis (n = 10; 23.25 %), hyperplastic gastritis (n = 8; 18.6 %), and combined gastritis (n = 15; 34.9 %). The focus was laid on identifying the main differentially significant endoscopic features specific to the gastritis-like form of primary gastric NHL which distinguish it from the lesions in other malignant tumors.
Conclusion. The complex examination using 4 concrete clarification methods of endoscopic diagnosis is indispensable to properly interpret the detected changes and timely diagnose the gastritis-like form of primary gastric NHL. These include narrow-band imaging (NBI/BLI and LCI), close-focus and magnification examinations, combined narrow-band imaging and magnification examination, as well as endosonography.
Universal geometry of two-neutron halos and Borromean Efimov states close to dissociation
Authors: Naidon Pascal 
Published: September 2023

The geometry of Borromean three-body halos, such as two-neutron halo nuclei or triatomic molecules close to dissociation, is investigated using a three-body model. This model enables to analytically derive the universal geometric properties found recently within an effective-field theory for halos made of a core and two resonantly-interacting particles [Phys. Rev. Lett., 128, 212501 (2022)]. It is shown that these properties not only apply to the ground three-body state, but also to all the excited (Efimov) states where the core-particle interaction is resonant. Furthermore, a universal geometry persists away from the resonant regime between the two particles, for any state close to the three-body threshold. This “halo universality”, which applies equally to all states, is different from the Efimov universality, which is only approximate for the ground state. It is explained by the separability of the hyper-radius and hyper-angles close to the three-body dissociation threshold.
The role of dermatocosmetics in the management of rosacea
Authors: Tserikidze N. G. , Sakaniya L. R. , Olenich I. V. , Korsunskaya I. M. 
Published: September 2023

Rosacea is a chronic inflammatory disease that affects the facial skin. This pathology is characterized by hyperaemia, erythema, telangiectasia and other manifestations. It is also accompanied by a group of unpleasant symptoms, such as burning sensations, itching, and skin tightness. The pathogenesis of the disease has not been sufficiently studied. But it is generally accepted that it is based on abnormalities in the immune system. In addition, excessive Demodex colonization and exposure to ultraviolet radiation may play a role in the development of rosacea. The incidence rate of rosacea worldwide reaches 5%. It is emphasized that some ethnicities face a greater risk of this disease than others. It is a very difficult task to treat rosacea for a specialist. The therapeutic approach differs in different forms of the disease. There is a wide range of treatments available for papulopustular rosacea, including systemic and topical medications and even physiotherapy. However, the therapy of erythematous rosacea mainly centres around the use of dermatocosmetic care. For patients with rosacea, it is important to choose products that, first of all, can effectively reduce skin redness and discomfort and will be well tolerated. A dermo-cosmetic composition has to have an anti-inflammatory effect and the ability to repair damaged skin. This article presents our own experience of using such drug in the monotherapy of erythematous rosacea and in the complex treatment of papulopustular disease. A significant effect had been achieved at 3–4 weeks of complex therapy in patients with papulopustular rosacea. We present a clinical case report of a 46-year-old patient N. as an example of the effectiveness of therapy.
Marker associations of chronic heart failure severity and cognitive dysfunction in elderly patients
Authors: Shishkova V. N. , Shishkov V. A. , Ustarbekova D. B. 
Published: September 2023

Introduction. Next to neurodegenerative disorders, cardiovascular diseases are now the most common cause of cognitive impairment. The combination of factors such as older age and chronic heart failure is a corner-stone of a greater risk for developing vascular cognitive impairment.Aim. To study the relationship between the parameters of the left ventricular ejection fraction and the concentration of NT-proBNP with the results of neuropsychological testing in patients with chronic heart failure in old age.Materials and methods. The study included 200 elderly patients with CHF II–III FC. The neuropsychological examination included tests: tracking, Schulte tables, verbal associations, the Montreal Cognitive Function Assessment Scale (МоСА test). Laboratory tests included determination of the concentration of NT-proBNP in serum.Results. During neuropsychological testing, reduced indicators were obtained: during the MOS test in patients with left ventricular ejection fraction (LVEF) values < 40% and ≥ 40% and < 50% and with a concentration of NT-proBNP 7230 [3325; 8830] pg/ml; in the Schulte test, an increase in execution time was noted in patients with LVEF values < 40% and ≥ 40% and < 50% and with a concentration of NT-proBNP 2900 [700; 7500] pg/ml; in the tracking test – an increase in time in part A in patients with LVEF values < 40% and ≥ 40% and < 50% and with a concentration of NT-proBNP 5385 [2125; 8675] pg/ml and part B in patients with LVEF values < 40% and ≥ 40% and < 50% and with a concentration of NT-proBNP 6947 [3325; 9310] pg/ml, in the verbal association test – in patients with LVEF values < 40% and ≥ 40% and < 50% and with a concentration of NT-proBNP 2090 [608; 7126] pg/ml. Correlation analysis showed the presence of a significant relationship between LVEF indicators, the concentration of NT-proBNP and the results of neuropsychological testing (p < 0.001), while, according to the Rea&Parker classification, the connection was assessed as relatively strong and medium strength.Conclusion. The cognitive impairments identified in this study in elderly patients with chronic heart failure were characterized by a decrease in concentration, memory, executive functions and the overall integrative index of cognitive functions. These disorders were significantly associated with a decrease in the left ventricular ejection fraction and a high concentration of NT-proBNP.
SOSYAL MEDYADA LİNÇ KÜLTÜRÜ VE YAŞLI AYRIMCILIĞI
Authors: GÖNÜLLÜ Canan 
Published: September 2023

Toplumsal bütünleşmenin önünde duran en büyük engellerden bir tanesi, toplumda var olan bir grup, kesim ya da topluluk hakkında geliştirilen ve yayılan önyargılar olabilmektedir. İletişimin ve etkileşimin arttığı günümüz toplumlarında da önyargıların yayılması ve toplumu etkileyebilir hale gelmesi çok kolay olabilmektedir.
Sosyal medyanın günümüzdeki kullanım şekliyle salt sosyal etkilerinden değil aynı zamanda hukuki dayanağı olan etkilerinden söz etmek de mümkün hale gelmiştir. Gerek kullanıcıların yeterli teorik bilgiye sahip olmadan paylaşımlarda bulunması gerekse de sosyal medya konusundaki hukuki düzenlemelerin henüz çok kapsamlı olmaması gerekçesiyle kimi zaman kişilerin kişilik haklarının ihlaline kadar varabilen ve çeşitli mağduriyetlere yol açan çeşitli durumların ortaya çıktığı gözlemlenebilmektedir. Belli bir grup, millet ya da kesim hakkında yapılan sosyal medya paylaşımları, toplumun birer parçası olan kimi kesimler hakkında peşin hüküm oluşmasına ve sonrasında bu kişilerin sosyal yaşantılarında, etiketlenmelerine ya da toplumdan tecrit edilmelerine yol açan aksaklıklara sebep olabilmektedir. Bu durumun da spesifik örnekler üzerinden toplumsal infial yaratabilecek ya da dijital linç adı verilen durum aracılığıyla yaşandığını görmek mümkündür.
Bu çalışmada yasal olarak sınırları çizilmiş olup suç olarak nitelendirilen ayrımcılığın; sosyal medyada yaşlılarla ilgili olan yönü incelenecektir. Öncelikle sosyal medya, kitle iletişim özgürlüğü ve dijital linç gibi konunun sosyal boyutu incelenecek ardından da konunun hukuki temelleri çalışmaya konu edilecektir. Derleme niteliğindeki bu çalışmanın amacı, literatürde linç kültürü olarak yer alan kavramla, hukuki olarak yaşlı ayrımcılığı şeklinde tanımlanmış kavram arasında yaşlılık sosyolojisi bakış açısıyla ilişki kurabilmektir. Bunun için, öncelikle kitle iletişim kavramı ve sosyal medyanın mahiyeti incelenmiş ardından da linç kültürü ve yaşlı ayrımcılığıyla ilgili hukuki dayanaklar ilişkilendirilmiştir. Çalışmanın sonucunda, sosyal mecralardaki söylem ve paylaşımların, ayrımcılığa neden olmak konusunda sosyolojik bir problem olabildiğine ulaşılmıştır.
Antagonistic Activity of Extremophilic Bacteria Against Phytopathogens in Agricultural Crops
Authors: Asyakina Lyudmila , Serazetdinova Yuliya , Frolova Anna , Fotina Natalya , Neverova Olga , Petrov Andrey 
Published: September 2023

Wheat is a vital agricultural crop whose phytopathogens include fungi of the genera Fusarium and Alternaria. Synthetic pesticides, which are used to combat them, have a negative impact on the environment. Therefore, there is a need for developing safe and effective biopesticides. We aimed to create a consortium of extremophilic microorganisms isolated from natural sources to protect wheat from the diseases caused by Alternaria and Fusarium fungi.
Ten isolates of extremophilic microorganisms were tested for their antimicrobial activity against Escherichia coli and their antagonistic activity against phytopathogens. Based on the results, we developed microbial consortia and evaluated their effectiveness in protecting wheat from phytopathogens.
Five of the strains under study showed the highest activity, three of which were biocompatible, namely Leclercia sp., Sphingomonas paucimobilis, and Lactobacillus plantarum. Four consortia were created from these microorganisms, of which consortium B (with a 2:1:1 ratio of the strains, respectively) proved the most effective. In particular, it increased the area free from the phytopathogen by 4.2% compared to the average values of its individual microorganisms. Also, the consortium had a phytostimulating effect on wheat seedlings (germination of 73.2–99.6%) and protected the seeds infected with phytopathogens from morphometric changes.
The resulting consortium can be used as a biopesticide since it is highly effective in protecting wheat from Alternaria and Fusarium pathogens.
Daily rainfall assimilation based on satellite and weather radar precipitation products along with rain gauge networks
Authors: Rodriguez-Ramirez Maria Asucena , Fuentes-Mariles Óscar Arturo 
Published: September 2023

Abstract

The analysis of the spatial and temporal distribution of storm events contributes to a better use of water resources, for example, the supply of drinking water, irrigation practices, electricity generation and management of extreme events to control floods and mitigate droughts, among others. The traditional observation of rainfall fields in Mexico has been carried out using rain gauge network data, but their spatial representativeness is unsatisfactory. Therefore, this study reviewed the possibility of obtaining better estimates of the spatial distribution of daily rainfall considering information from three different databases, which include rain gauge measurements and remotely sensed precipitation products of satellite systems and weather radars. In order to determine a two-dimensional rainfall distribution, the information has been merged with a sequential data assimilation scheme up to the diagnostic stage, paying attention to the benefit that the rain gauge network density has on the estimation. With the application of the Barnes method, historical events in the Mexican territory were analyzed using statistical parameters for the validation of the estimates, with satisfactory results because the assimilated rainfalls turned out to be better approximations than the values calculated with the individual databases, even for a not very low density of surface observations.
Evaluating 2021 extreme flash flood of Teesta River
Authors: Shampa  , Nejhum Israt Jahan , Hussain Md. Manjurul , Islam Mohammad Muddassir , Zoha Rubaid Hassan 
Published: September 2023

Abstract

Due to global warming, extreme hydroclimatic events (e.g., floods) are expected to happen more frequently and last longer. This study investigated such an extreme flood in the transboundary Teesta River that occurred in October 2021. We attempted to quantify the event's impact using data from time series flood levels, precipitation-related satellite images, and two-dimensional hydromorphological modeling. We found it challenging for people to cope with such a hazardous event since the depth of the flooding increased 6.98-fold in just 24 h. Our simulation results indicate that a sand-filled sediment measuring 0.27 m thick covered more than 33% cropland, and the velocity increased by almost 2.5 times. 136,000 individuals were marooned in the water. Compared to previous flooding events in its basin, which occurred in India and Bangladesh, the river appears to have some natural shock absorption features, i.e., a wide braided plain. We propose impact-based forecasting with a proactive early response as a valuable tool for managing such extreme events.
Ambient Noise Tomography studies for geothermal exploration in the Canary Islands
Authors: Cabrera-Pérez Iván , D’Auria Luca , Soubestre Jean , Martínez van Dorth David , Cervigón-Tomico Germán , Martín-Lorenzo Alba , Przeor Monika , García-Hernández Rubén , Ortega Víctor , Padilla Germán D. , Barrancos José , Padrón Eleazar , Pérez Nemesio M. 
Published: September 2023

Ambient Noise Tomography is a geophysical exploration technique that has proven to be highly efficient for studies at different scales and for multiple purposes, such as geothermal exploration. In this article, we introduce this technique by reviewing its various steps. Additionally, we present some examples of applications from studies conducted in the Canary Islands (specifically in Tenerife, Gran Canaria, and La Palma) for geothermal exploration purposes.The study realized in Gran Canaria reveals a series of low-velocity zones in the southern and eastern parts of the island, which could be linked to convective cells. In Tenerife, a low-velocity zone has been observed, potentially associated with a superficial clay cap that could facilitate the ascent of gases to the surface. Finally, the study carried out in La Palma highlights the existence of two low-velocity zones in the southern part of the island, possibly related to hydrothermally altered clay zones, indicating a circulation of hydrothermal fluids.
The HYDROTHERM code as a tool for integrating geophysics in geothermal prospecting
Authors: García de la Noceda Celestino , Albert Beltrán José F. 
Published: September 2023

The use of numerical codes in simulating geothermal reservoirs is one of the most powerful tools for integrating geology, geochemistry, and geophysics in high enthalpy geothermal research processes. This paper summarizes some examples of USGS HYDROTHERM code application results on the islands of Gran Canaria, Lanzarote, and Tenerife, which are considered very different geothermal models. The first island has normal geothermal gradient, the second has magmatic bodies near the surface and Tenerife has a comparison between geophysical techniques to determine the recharge-discharge flows of the Cañadas-Teide system. In the first two islands there are boreholes that allow adjusting and validating the models, whereas Tenerife or La Palma have been studied using the same methodology but lack verification boreholes. Nevertheless, their model results are consistent with other geophysical techniques. This highlights the great potential of this code as an integrating tool for the different prospecting techniques to understand geothermal system operations and determine the most suitable location for deep exploration drilling. The significant benefit of this code is the capability to reproduce different evolutionary and casuistry models reliably using the same geological and thermal parameters for all islands.
COMBINATIVE FRAMEWORK FOR VALUE ENGINEERING AND BUILDING INFORMATION MODELLING IMPLEMENTATION AT LOD300 STAGE
Authors: Zaidi Ahmad Aqil , Abd-Karim Saipol Bari , Fariq Nazirul , Yun Hsiao 
Published: September 2023

Value Engineering (VE) involves a multidisciplinary team approach in a highly systematic workshop aimed at achieving Value for Money (VfM) focused on improving the function of a project, product, or service, not merely reducing costs. In the construction industry, VE is applied during the design or pre-construction phases of public, private, or Building Information Modelling (BIM)-based projects where benefits can be maximised. VE can be implemented more effectively with BIM, especially in providing accurate and adequate information. It is important to understand how these two concepts are related and how they benefit projects. Nevertheless, there is a dearth of research that combines VE practice with BIM to evaluate viable alternatives or improve design. This paper investigated the need for VE in the LOD300 phase for construction projects in Malaysia. In this work, a quantitative methodology was used to incorporate findings from the literature into a questionnaire survey that was purposefully distributed to industry practitioners with knowledge of VE, BIM, or both. A sample of 186 was drawn from a total population of 353 professionals: Members of the Institute of Value Management Malaysia (IVMM), Public Works Department of Malaysia (PWD) VM and BIM, and myBIM Centre Malaysia. 32% of responses were received, and the data collected were analysed using the Statistical Package for Social Sciences (SPSS). Based on the results, a framework was developed to include VE at the LOD300 phase in BIM. This paper provides empirical evidence of the benefits of this approach and offers construction practitioners with an overview of how VE can be systematically applied to BIM.