bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZnO nanorods anchored on CNTs incorporating carbon cloth flexible electrode as a highly sensitive electrochemical sensor
Authors: Shi Fengna , Li Jingfang , Chen Cheng , Wang Fang , Chen Naipin , Xu Ziqi , Wang Jiajun 
Published: June 2023

Abstract
Monitoring glucose, uric acid (UA) and hydrogen peroxide (H2O2) concentration has emerged as a critical health care issue to prevent acute complications and to minimize the hazard of long-term complications. In this paper, a novel non-enzyme electrochemical sensor was proposed with nanorod-like zinc oxide anchored on carbon nanotubes using a direct precipitation method and then decorated onto carbon cloth (ZnO/CNTs/CC). The ZnO/CNTs composite was characterized by x-ray photoelectron spectroscopy (XPS), Raman spectrum, TEM microscope and electrochemistry. The sensing of UA, glucose and H2O2 individually or simultaneously was done on a ZnO/CNTs/CC electrode, and the superior performance lies in its wide linear range, low detection limit and high selectivity, which is attributed to the synergistic effect of (a) the good electrocatalytic activity of ZnO nanorods, and (b) the large surface area with high conductivity offered by CNTs. Moreover, the ZnO/CNTs/CC electrode showed good reproducibility, stability and selectivity. Importantly, the developed sensor platform has been successfully applied to probe glucose, UA and H2O2 in human serum with satisfactory recoveries. Our proposed approach is simple in fabrication and operation, which provides a straightforward assay for the reliable and cost-effective determination of glucose, UA and H2O2 in clinical diagnosis and biomedical applications.
Construction of organic–inorganic hybrid heterostructure towards solvent responsive hydrogel with high stiffness
Authors: Wang Junju , Tang Jie , Lin Yichao , He Hong , Zhao Chaoshan , Ma Wenrui , Wang Xiang , Zeng Muling , Li Shunbo 
Published: June 2023

Abstract
Solvent responsive hydrogel is a kind of intelligent soft material, which can be used in soft robots. Currently, most of the solvent responsive hydrogels are based on pure organic materials which has limited stiffness for actuations. Herein, a novel organic–inorganic composite hydrogel is designed and prepared. Calcium phosphate oligomers nanoclusters are incorporated in polymer solution containing polyvinyl alcohol (PVA) and sodium alginate to form organic–inorganic hybrid copolymer suspensions. The solvent responsive hydrogel is simply prepared using co-evaporation method by optimizing the components in the colloid system. The inorganic nanoparticles work as the scaffold in the porous PVA network and the evaporation caused nonuniformity distribution further induces the formation of heterostructure, which has different shrinkage ratios along the thickness direction. The prepared hydrogel demonstrates excellent shape memory property by changing the environmental solvents between water and ethanol and its repeatability is also verified. The stiffness of hydrogel is enhanced and it has large deformation after incorporation of calcium phosphate nanoparticles. The bending angle of hydrogel can be well controlled by different water to ethanol ratios, allowing for underwater actuation. The functionality of an artificial gripper based on responsive hydrogel with high stiffness is demonstrated to transfer objects in ethanol. The design of organic–inorganic composite hydrogel with high stiffness may provide new insights for preparation of intelligent soft materials for underwater applications.
A study on stochastic longitudinal wave equation in a magneto-electro-elastic annular bar to find the analytical solutions
Authors: Miah M Mamun , Iqbal M Ashik , Osman M S 
Published: June 2023

Abstract
In this paper, we set up dynamic solitary perturb solutions of a unidirectional stochastic longitudinal wave equation in a magneto-electro-elastic annular bar by a feasible, useful, and influential method named the dual

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/
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,

1
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-expansion method. Computer software, like Mathematica, is used to complete this discussion. The obtained solutions of the proposed equation are classified into trigonometric, hyperbolic, and rational types which play an important role in searching for numerous scientific events. The technique employed here is an extension of the (

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′

/
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)-expansion technique for finding all previously discovered solutions. To illustrate our findings more clearly, we provide 2D and 3D charts of the various recovery methods. We then contrasted our findings with those of past solutions. The graphical illustrations of the acquired solutions are singular periodic solitons and kink solitons which are added at the end of this paper.
MoS2 nanocomposites for biomolecular sensing, disease monitoring, and therapeutic applications
Authors: Harini Karthick , Girigoswami Koyeli , Pallavi Pragya , Gowtham Pemula , Thirumalai Anbazhagan , Charulekha Kamalakkannan , Girigoswami Agnishwar 
Published: June 2023

Abstract
The unique physicochemical properties of MoS2 nanocomposites have drawn escalation in attention for the diagnosis and therapy of cancer. Mostly the 2D forms of MoS2 find application in sensing, catalysis, and theranostics, where it was traditionally applied in lubrication and battery industries as electrodes or intercalating agents. As nanostructures, MoS2 has a very high surface-to-volume ratio, and that helps in the engineering of structures and surfaces to promote absorption of a wide range of therapeutics and biomolecules through covalent or non-covalent interaction. This surface engineering provides excellent colloidal stability to MoS2 and makes them ideal nanomedicines with higher selectivity, sensitivity, and biomarker sensing ability. Furthermore, MoS2 exhibits exceptionally well optical absorption of NIR radiation and photothermal conversion, which helps in the NIR-responsive release of payloads in photothermal and photodynamic therapy. There are several reports that the fabricated MoS2 nanomedicines can selectively counter the tumor microenvironment, which leads to the accumulation of therapeutics or imaging agents in the diseased tissues to improve the therapeutic effects decreasing the adverse effects on the healthy cells. An overview of the basic structure and properties of MoS2 is presented in this article, along with an elaborative description of its morphology. At the same time, an attempt was made in this review to summarize the latest developments in the MoS2 structure, surface engineering, and nanocomposite formulations for improving biocompatibility, bioavailability, biomolecular sensing, and theranostic applications.
A Driver in Health Outcomes: Developing Discrete Categories of Transportation Insecurity
Authors: McDonald-Lopez Karina , Murphy Alexandra K , Gould-Werth Alix , Griffin Jamie , Bader Michael D M , Kovski Nicole 
Published: June 2023

Abstract
Research suggests that transportation is an important social determinant of health, because the ability to get around is consequential for accessing health care and nutritious food and for making social connections. We used an inductive mixed-methods approach and a quantitative k-means clustering approach to identify 5 categories of transportation insecurity using the validated 16-item Transportation Security Index. The resulting 5-category measure distinguished among respondents with qualitatively different experiences of transportation insecurity. Analyzing data from 2018 that were representative of the US adult population aged 25 years or older, we demonstrated a nonparametric association between transportation insecurity and 2 different health measures (self-rated health and depressive symptoms). There was a threshold relationship between self-rated health and any level of transportation insecurity. High transportation insecurity had a very strong relationship with depressive symptoms. The categorical Transportation Security Index will be useful for clinicians who wish to screen for transportation-related barriers to health care. It will also facilitate research investigating the influence of transportation insecurity on health outcomes and provide the basis for interventions designed to address health disparities.
An update on drug-drug interactions for care of the acutely ill in the era of COVID-19
Authors: Patanwala Asad E , Jager Nynke G L , Radosevich John J , Brüggemann Roger 
Published: June 2023

Abstract

Purpose
To provide key pharmacological concepts underlying drug-drug interactions (DDIs), a decision-making framework, and a list of DDIs that should be considered in the context of contemporary acutely ill patients with COVID-19.

Summary
DDIs are frequently encountered in the acutely ill. The implications of DDIs include either increased risk of drug toxicity or decreased effectiveness, which may have severe consequences in the acutely ill due to lower physiological and neurocognitive reserves in these patients. In addition, an array of additional therapies and drug classes have been used for COVID-19 that were not typically used in the acute care setting. In this update on DDIs in the acutely ill, we provide key pharmacological concepts underlying DDIs, including a discussion of the gastric environment, the cytochrome P-450 (CYP) isozyme system, transporters, and pharmacodynamics in relation to DDIs. We also provide a decision-making framework that elucidates the identification of DDIs, risk assessment, selection of alternative therapies, and monitoring. Finally, important DDIs pertaining to contemporary acute care clinical practice related to COVID-19 are discussed.

Conclusion
Interpreting and managing DDIs should follow a pharmacologically based approach and a systematic decision-making process to optimize patient outcomes.
The optimal time to approach an unfamiliar object: a Bayesian model
Authors: Sherratt Tom N , Dewan Ian , Skelhorn John 
Published: June 2023

AbstractMany organisms take time before approaching unfamiliar objects. This caution forms the basis of some well-known assays in the fields of behavioral ecology, comparative psychology, and animal welfare, including quantifying the personality traits of individuals and evaluating the extent of their neophobia. In this paper, we present a mathematical model which identifies the optimal time an observer should wait before approaching an unfamiliar object. The model is Bayesian, and simply assumes that the longer the observer goes without being attacked by an unfamiliar object, the lower will be the observer’s estimated probability that the object is dangerous. Given the information gained, a time is reached at which the expected benefits from approaching the object begin to exceed the costs. The model not only explains why latency to approach may be repeatable among individuals and varies with the object’s appearance but also why individuals habituate to the stimulus, approaching it more rapidly over repeated trials. We demonstrate the applicability of our model by fitting it to published data on the time taken by chicks to attack artificial caterpillars that share no, one, or two signaling traits with snakes (eyespots and posture). We use this example to show that while the optimal time to attack an unfamiliar object reflects the observer’s expectation that the object is dangerous, the rate at which habituation arises is also a function of the observer’s certainty in their belief. In so doing, we explain why observers become more rapidly habituated to “weaker” stimuli than the “stronger” ones.
Two-Year Outcomes of Treatment-Experienced Adults After Programmatic Transitioning to Dolutegravir: Longitudinal Data From the VICONEL Human Immunodeficiency Virus Cohort in Lesotho
Authors: Tschumi Nadine , Lerotholi Malebanye , Motaboli Lipontso , Mokete Moliehi , Labhardt Niklaus D , Brown Jennifer A 
Published: June 2023

Abstract
In the Viral Load Cohort North-East Lesotho (VICONEL) human immunodeficiency virus cohort, 14 242 adults had transitioned from efavirenz- or nevirapine-based antiretroviral therapy (ART) to dolutegravir-based ART by October 2021. Rates of viral suppression to &lt;50 copies/mL were 84.8%, 93.9%, and 95.4% before, 12 months after, and 24 months after transition, respectively. Sex, age, pretransition viral load, and treatment backbone correlated with 24-month viremia.
Time, equilibrium and uncertainty: Bergson and Robinson
Authors: Culham James 
Published: June 2023

Abstract
The philosophy of Henri Bergson can lend fresh perspectives on some central aspects of post-Keynesian economic thought. Bergson’s concept of duration offers philosophical reinforcement for Joan Robinson’s criticisms of the treatment of time, equilibrium and uncertainty in economics. When the economy is recognised to be a dynamic living system, in which the accumulation of capital is an historical process inseparable from technical innovation, the effect of time is of utmost importance. Duration provides greater understanding of an economy moving through historical time, while adding depth to Robinson’s doubts about the validity of utility, and consequently expected utility theory. Bergson’s philosophy of evolution and duration provides a valuable basis for understanding many of the classical issues in political economy.
Design and methodology of the PRIMETIME 1 cohort study: PRecIsion MEdicine based on kidney TIssue Molecular interrogation in diabetic nEphropathy
Authors: Jensen Karina Haar , Persson Frederik , Hansen Ditte , Bressendorff Iain , Møller Marie , Rossing Peter , Gravesen Eva , Kosjerina Vanja , Vistisen Dorte , Borg Rikke 
Published: June 2023

ABSTRACT

Background
Clinical features of diabetic kidney disease alone cannot differentiate between the histopathology that defines diabetic nephropathy (DN) and non-diabetic nephropathy (NDN). A kidney biopsy is necessary to make the definitive diagnosis of DN. However, there is no consensus on when to perform a kidney biopsy in individuals with diabetes and kidney disease. Furthermore, the implications of NDN versus DN for management, morbidity and kidney prognosis are unclear. To address the gap in knowledge, we aimed to create a national retrospective cohort of people with diabetes and a performed kidney biopsy.

Methods
Adults diagnosed with diabetes in Denmark between 1996 and 2020 who had a kidney biopsy performed were included. The cohort was established by linking a nationwide diabetes registry with the Danish Pathology Registry. Data from 11 national registries and databases were compiled. The type of kidney disease was classified using a three-step analysis of Systematized Nomenclature of Medicine codes reported in relation to the histopathological examinations of kidney tissue. The final cohort and classification of kidney disease was as follows: out of 485 989 individuals with diabetes 2586 were included, 2259 of whom had type 2 diabetes. We were able to classify 599 (26.5%) with DN, 703 (31.1%) with NDN and 165 (7.3%) with mixed disease in individuals with type 2 diabetes. In individuals with type 1 diabetes, 132 (40.4%) had DN, 73 (22.3%) NDN and 39 (11.9%) mixed disease. The remaining could not be classified or had normal histology. The overall median (Q1–Q3) follow-up time was 3.8 (1.6–7.2) years.

Conclusions
This cohort is a novel platform based on high-quality registry data for important longitudinal studies of the impact of kidney disease diagnosis on prognosis. With regular updates of data from the Danish registries, the presented follow-up will increase over time and is only limited by emigration or death.
Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula
Authors: Lodi Rathna Silviya , Dong Xiaodan , Jiang Chunhui , Sun Zhou , Deng Peng , Sun Sujun , Wang Xinkun , Wang Hengzhen , Mesa Arundathi , Huang Xiaopeng , Peng Lizeng , Peng Chune 
Published: June 2023

Abstract
The roots of the medicinal plant Codonopsis pilosula (Franch.) Nannf (C. pilosula) possess most medicinal supplements. In current research on C. pilosula root endophytes were isolated, identified, and evaluated for their antimicrobial activity against human pathogens such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Pseudomonas aeruginosa and the fungi Candida albicans and Aspergillus niger. Endophytes C.P-8 and C.P-20 exhibited very significant antimicrobial activity, the secondary metabolite of C.P-8 registered at retention time 24.075 by HPLC analysis. Significant minimum inhibitory concentration (MIC) of C.P-8 was exhibited at 250 µg/ml against S. aureus and 500 µg/ml against B. subtilis. Qualitative, quantitative analyses, and partial purification of enzymes and purity was analysed by molecular weight determined by SDS‒PAGE of enzymes produced by C.P-20, amylase—64 kDa, protease—64 kDa, chitinase—30 kDa, and cellulase—54 kDa. Optimum pH and temperature of the partially purified enzymes, was carried out. The partially purified enzymes from C.P-20 displayed maximum activity at pH 6–7 and temperatures of 40°C–45°C. Moreover, the above endophytes will be useful tools for producing active enzymes and active bioantimicrobial agents against human pathogens.
Role of DevR phosphorylation in nitric oxide homeostasis and signaling ofStreptomyces coelicolorA3(2) M145
Authors: Honma Sota , Ito Shinsaku , Yajima Shunsuke , Sasaki Yasuyuki 
Published: June 2023

AbstractOur previous studies revealed that a two-component system (TCS), DevS, and DevR, regulate both nitric oxide (NO) signaling and NO homeostasis in the actinobacterium Streptomyces coelicolor A3(2) M145, suggesting a reasonable system for NO-dependent metabolism. In this study, sequence alignment of DevR and DevR homologs found Asp66 (D66) and Thr196 (T196) as predicted phosphorylation sites of DevR. Phos-tag gel electrophoretic mobility shift assay suggested that D66 and T196 are involved in the phosphorylation of DevR. The respective point mutations of D66 and T196 significantly decreased the transcriptional activity of DevR, which affected nitrite production and aerial mycelium formation. These results suggested that both D66 and T196 of DevR are important for the regulation of NO homeostasis and signaling in S. coelicolor A3(2) M145.
Identification of transcriptional regulatory variants in pig duodenum, liver, and muscle tissues
Authors: Crespo-Piazuelo Daniel , Acloque Hervé , González-Rodríguez Olga , Mongellaz Mayrone , Mercat Marie-José , Bink Marco C A M , Huisman Abe E , Ramayo-Caldas Yuliaxis , Sánchez Juan Pablo , Ballester Maria 
Published: June 2023

Abstract

Background
In humans and livestock species, genome-wide association studies (GWAS) have been applied to study the association between variants distributed across the genome and a phenotype of interest. To discover genetic polymorphisms affecting the duodenum, liver, and muscle transcriptomes of 300 pigs from 3 different breeds (Duroc, Landrace, and Large White), we performed expression GWAS between 25,315,878 polymorphisms and the expression of 13,891 genes in duodenum, 12,748 genes in liver, and 11,617 genes in muscle.

Results
More than 9.68 × 1011 association tests were performed, yielding 14,096,080 significantly associated variants, which were grouped in 26,414 expression quantitative trait locus (eQTL) regions. Over 56% of the variants were within 1 Mb of their associated gene. In addition to the 100-kb region upstream of the transcription start site, we identified the importance of the 100-kb region downstream of the 3′UTR for gene regulation, as most of the cis-regulatory variants were located within these 2 regions. We also observed 39,874 hotspot regulatory polymorphisms associated with the expression of 10 or more genes that could modify the protein structure or the expression of a regulator gene. In addition, 2 motifs (5′-GATCCNGYGTTGCYG-3′ and a poly(A) sequence) were enriched across the 3 tissues within the neighboring sequences of the most significant single-nucleotide polymorphisms in each cis-eQTL region.

Conclusions
The 14 million significant associations obtained in this study are publicly available and have enabled the identification of expression-associated cis-, trans-, and hotspot regulatory variants within and across tissues, thus shedding light on the molecular mechanisms of regulatory variations that shape end-trait phenotypes.
5-Aminolevulinic acid bypasses mitochondrial complex I deficiency and corrects physiological dysfunctions in Drosophila
Authors: Nozawa Naoko , Noguchi Marie , Shinno Kanako , Saito Taro , Asada Akiko , Ishii Takuya , Takahashi Kiwamu , Ishizuka Masahiro , Ando Kanae 
Published: June 2023

Abstract
Complex I (CI) deficiency in mitochondrial oxidative phosphorylation (OXPHOS) is the most common cause of mitochondrial diseases, and limited evidence-based treatment options exist. Although CI provides the most electrons to OXPHOS, complex II (CII) is another entry point of electrons. Enhancement of this pathway may compensate for a loss of CI; however, the effects of boosting CII activity on CI deficiency are unclear at the animal level. 5-Aminolevulinic acid (5-ALA) is a crucial precursor of heme, which is essential for CII, complex III, complex IV (CIV) and cytochrome c activities. Here, we show that feeding a combination of 5-ALA hydrochloride and sodium ferrous citrate (5-ALA-HCl + SFC) increases ATP production and suppresses defective phenotypes in Drosophila with CI deficiency. Knockdown of sicily, a Drosophila homolog of the critical CI assembly protein NDUFAF6, caused CI deficiency, accumulation of lactate and pyruvate and detrimental phenotypes such as abnormal neuromuscular junction development, locomotor dysfunctions and premature death. 5-ALA-HCl + SFC feeding increased ATP levels without recovery of CI activity. The activities of CII and CIV were upregulated, and accumulation of lactate and pyruvate was suppressed. 5-ALA-HCl + SFC feeding improved neuromuscular junction development and locomotor functions in sicily-knockdown flies. These results suggest that 5-ALA-HCl + SFC shifts metabolic programs to cope with CI deficiency.
Bullet outline 5-Aminolevulinic acid (5-ALA-HCl + SFC) increases ATP production in flies with complex I deficiency.5-ALA-HCl + SFC increases the activities of complexes II and IV.5-ALA-HCl + SFC corrects metabolic abnormalities and suppresses the detrimental phenotypes caused by complex I deficiency.
Non-Bilaterians as Model Systems for Tissue Mechanics
Authors: Gooshvar Setareh , Madhu Gopika , Ruszczyk Melissa , Prakash Vivek N 
Published: June 2023

Synopsis
In animals, epithelial tissues are barriers against the external environment, providing protection against biological, chemical, and physical damage. Depending on the organism’s physiology and behavior, these tissues encounter different types of mechanical forces and need to provide a suitable adaptive response to ensure success. Therefore, understanding tissue mechanics in different contexts is an important research area. Here, we review recent tissue mechanics discoveries in three early divergent non-bilaterian systems—Trichoplax adhaerens, Hydra vulgaris, and Aurelia aurita. We highlight each animal’s simple body plan and biology and unique, rapid tissue remodeling phenomena that play a crucial role in its physiology. We also discuss the emergent large-scale mechanics in these systems that arise from small-scale phenomena. Finally, we emphasize the potential of these non-bilaterian animals to be model systems in a bottom-up approach for further investigation in tissue mechanics.