bluuebunny/crossref_metadata_2025_split huggingface dataset:
A genetic toolbox for the turquoise killifish identifies sporadic age-related cancer
Authors: Rozenberg Itai , Franek Roman , Moses Eitan , Atlan Tehila , Diehl Mathias , Oron-Gottesman Adi , Bergman Marva , Astre Gwendoline , Rosental Benyamin , Goshtchevsky Uri , von Chrzanowski Henrik , Harel Itamar 
Published: May 2023

AbstractAging is accompanied by a progressive loss of tissue homeostasis, including declining stem-cell function and increased cancer susceptibility. The naturally short-lived African turquoise killifish has emerged as a powerful system for investigating vertebrate aging. However, a critical mass of advanced genetic tools for mechanistic studies has been largely missing. Here, we develop theKillibow, a multispectral transgenic line for life-long lineage tracing, an immunocompromisedrag2mutant for transplantation studies, and genetic mutants for investigating genomic instability and cancer (i.e.atmandtp53). We performed a series of experiments using this platform, including lineage tracing following germline stem-cell transplantation, and identifying naturally occurring age-related melanoma using engraftment intorag2mutants. Exploring tumor dynamics reveals an intriguing age-related interplay between genomic instability and adaptive immunity, accompanied by an evolutionarily conserved decline in immune functions. Together, this toolkit streamlines the investigation of the molecular mechanisms underlying the loss of tissue homeostasis during aging and disease.
Only three principal components account for inter-embryo variability of the spindle length over time
Authors: Cunff Yann Le , Chesneau Laurent , Pastezeur Sylvain , Pinson Xavier , Soler Nina , Fairbrass Danielle , Mercat Benjamin , Garcia Ruddi Rodriguez , Alayan Zahraa , Abdouni Ahmed , de Neidhardt Gary , Costes Valentin , Anjubault Mélodie , Bouvrais Hélène , Héligon Christophe , Pécréaux Jacques 
Published: May 2023

AbstractHow does inter-individual variability emerge? When measuring a large number of features per experiment/individual, this question becomes non-trivial. One challenge lies in choosing features to recapitulate high-dimension data. In this paper, we focus on spindle elongation phenotype to highlight how a data-driven approach can help. We showed that only three typical elongation patterns could describe spindle elongation in C.elegansone-cell embryo. We called them archetypes. These archetypes were automatically extracted from the experimental data using principal component analysis (PCA) rather than defined a priori. They accounted for more than 85% of inter-individual variability in a dataset of more than 1600 experiments across more than 100 different experimental conditions (RNAi, mutants, changes in temperature, etc.). The two first archetypes were consistent with standard measures in the field, namely the average spindle length and the spindle elongation rate both in late metaphase and anaphase. However, our archetypes were not strictly corresponding to these manually-set features. The third archetype, accounting for 6% of the variance, was novel and corresponded to a transient spindle shortening in late metaphase. We propose that it is part of spindle elongation dynamics in all conditions. It is reminiscent of the elongation pattern observed upon defects in kinetochore function. Notably, the same archetypes emerged when analysing non-treated embryos only at various temperatures. Interestingly, because these archetypes were not specific to metaphase or anaphase, it implied that spindle elongation around anaphase-onset is sufficient to predict its late anaphase length. We validated this idea using a machine-learning approach.Despite the apparent variability in phenotypes across the various conditions, inter-individual differences between embryos depleted from one cell division-related protein have the same underlying nature as inter-individual differences naturally arising between wild-type embryos. The same conclusion holds when analysing embryos dividing at various temperatures. We thus propose that beyond the apparent complexity of the spindle, only three independent mechanisms account for spindle elongation, and contribute differently in the various conditions, meanwhile, no mechanism is specific to any condition.
Genomic survey maps differences in the molecular complement of vesicle formation machinery betweenGiardia intestinalisassemblages
Authors: Pipaliya Shweta V. , Dacks Joel B. , Croxen Matthew A. 
Published: May 2023

ABSTRACTGiardia intestinalisis a globally important microbial pathogen with considerable public health, agricultural, and economic burden. Genome sequencing and comparative analyses have elucidatedGiardia intestinalisto be a taxonomically diverse species consisting of at least eight different sub-types (assemblages A-H) that can infect a great variety of animal hosts, including humans. The best studied of these are assemblages A and B which have a broad host range and have zoonotic transmissibility towards humans where clinical Giardiasis can range from asymptomatic to diarrheal disease. Epidemiological surveys as well as previous molecular investigations have pointed towards critical genomic level differences within numerous molecular pathways and families of parasite virulence factors within assemblage A and B isolates.In this study, we explored the necessary machinery for the formation of vesicles and cargo transport in 89 Canadian isolates of assemblage A and BGiardia intestinalis. There is considerable variability within the molecular complement of the endolysosomal ESCRT protein machinery, adaptor coat protein complexes, and ARF regulatory system. We report inter-assemblage, but no intra-assemblage variation within the trafficking systems examined. These include losses of subunits belonging to the ESCRTIII as well as novel lineage specific duplications in components of the COPII machinery, ARF1, and ARFGEF families (BIG and CYTH). Since assemblages A and B show differences in disease manifestation, our findings may well have clinical implications and even taxonomic, as the membrane trafficking system underpin parasite survival, pathogenesis, and propagation.
Orthogonal CRISPR screens to identify transcriptional and epigenetic regulators of human CD8 T cell function
Authors: McCutcheon Sean R. , Swartz Adam M. , Brown Michael C. , Barrera Alejandro , McRoberts Amador Christian , Siklenka Keith , Humayun Lucas , Isaacs James M. , Reddy Timothy E. , Nair Smita , Antonia Scott , Gersbach Charles A. 
Published: May 2023

AbstractThe clinical response to adoptive T cell therapies is strongly associated with transcriptional and epigenetic state. Thus, technologies to discover regulators of T cell gene networks and their corresponding phenotypes have great potential to improve the efficacy of T cell therapies. We developed pooled CRISPR screening approaches with compact epigenome editors to systematically profile the effects of activation and repression of 120 transcription factors and epigenetic modifiers on human CD8+ T cell state. These screens nominated known and novel regulators of T cell phenotypes with BATF3 emerging as a high confidence gene in both screens. We found that BATF3 overexpression promoted specific features of memory T cells such as increased IL7R expression and glycolytic capacity, while attenuating gene programs associated with cytotoxicity, regulatory T cell function, and T cell exhaustion. In the context of chronic antigen stimulation, BATF3 overexpression countered phenotypic and epigenetic signatures of T cell exhaustion. CAR T cells overexpressing BATF3 significantly outperformed control CAR T cells in both in vitro and in vivo tumor models. Moreover, we found that BATF3 programmed a transcriptional profile that correlated with positive clinical response to adoptive T cell therapy. Finally, we performed CRISPR knockout screens with and without BATF3 overexpression to define co-factors and downstream factors of BATF3, as well as other therapeutic targets. These screens pointed to a model where BATF3 interacts with JUNB and IRF4 to regulate gene expression and illuminated several other novel targets for further investigation.
Embedding stochastic dynamics of the environment in spontaneous activity by prediction-based plasticity
Authors: Asabuki Toshitake , Clopath Claudia 
Published: May 2023

AbstractThe brain learns an internal model of the environment through sensory experiences, which is essential for high-level cognitive processes. Recent studies show that spontaneous activity reflects such learned internal model. Although computational studies have proposed that Hebbian plasticity can learn the switching dynamics of replayed activities, it is still challenging to learn dynamic spontaneous activity that obeys the statistical properties of sensory experience. Here, we propose a pair of biologically plausible plasticity rules for excitatory and inhibitory synapses in a recurrent spiking neural network model to embed stochastic dynamics in spontaneous activity. The proposed synaptic plasticity rule for excitatory synapses seeks to minimize the discrepancy between stimulus-evoked and internally predicted activity, while inhibitory plasticity maintains the excitatory-inhibitory balance. We show that the spontaneous reactivation of cell assemblies follows the transition statistics of the model’s evoked dynamics. We also demonstrate that simulations of our model can replicate recent experimental results of spontaneous activity in songbirds, suggesting that the proposed plasticity rule might underlie the mechanism by which animals learn internal models of the environment.
Glutamate Transporters are Involved in Direct Inhibitory Synaptic Transmission in the Vertebrate Retina
Authors: Niklaus Stephanie , Glasauer Stella M.K. , Kovermann Peter , Farshori Kulsum F. , Cadetti Lucia , Früh Simon , Rieser Nicolas N. , Gesemann Matthias , Fahlke Christoph , Neuhauss Stephan C.F. 
Published: May 2023

AbstractIn the central nervous system of vertebrates, glutamate serves as the primary excitatory neurotransmitter. However, in the retina, glutamate released from photoreceptors causes hyperpolarization in postsynaptic ON-bipolar cells through a glutamate-gated chloride current, which seems paradoxical. Our research reveals that this current is modulated by two excitatory glutamate transporters, EAAT5b and EAAT7. These transporters are located at the dendritic tips of ON-bipolar cells and interact with all four types of cone photoreceptors. The absence of these transporters leads to a decrease in ON-bipolar cell responses, with eaat5b mutants being more severely affected than eaat5b/eaat7 double mutants, which also exhibit altered response kinetics. Biophysical investigations establish that EAAT7 is an active glutamate transporter with a predominant anion conductance. Our study is the first to demonstrate the direct involvement of postsynaptic glutamate transporters in inhibitory direct synaptic transmission at a central nervous system synapse.
The metaverse experience in luxury brands
Authors: Jiang Qi , Kim Miyea , Ko Eunju , Kim Kyung Hoon 
Published: April 2023

PurposeThe purpose of this study is to develop the scale of the metaverse experience and examine the effect of the metaverse experience on consumer happiness in luxury brands.Design/methodology/approachA survey was distributed to metaverse users from general participants in the Republic of Korea. To reach this goal, the domain of the metaverse experience is defined, items are created through qualitative interviews, the initial scale is refined and then the scale is tested. A total of 262 valid responses were used for exploratory factor analysis (EFA), and 238 data were used for confirmatory factor analysis (CFA). The hypothesis was tested using structural equation modeling (SEM) and SmartPLS 3.0.FindingsThe results showed that the metaverse experience comprises three sub-constructs, namely fantasies, feelings and fun. Also, the metaverse experience affects consumer happiness positively in luxury brands.Originality/valueThis study investigates consumer experience in the metaverse environment. The authors examined the metaverse experience based on the experiential aspects of the consumption of luxury brands and develop the scale development of the metaverse experience. Furthermore, the relationship between metaverse experiences and consumer happiness provides a new perspective in studying metaverse marketing strategy and customer service creation.
The effects of diningscape on customer satisfaction and word of mouth
Authors: To Wai Ming , Leung Vincent W.S. 
Published: April 2023

PurposeRestaurant dining is an important part of people's live, and the restaurant industry is one of the largest industries in the hospitality sector. Thus, this study explores the effects of restaurant diningscape on customer satisfaction and word of mouth.Design/methodology/approachBased on a literature review on restaurant servicescape and special functions of dining spaces, diningscape was conceptualized as a multidimensional construct. Data were collected from 378 restaurant patrons using snowball sampling in Macao, China. The validity and reliability of constructs were assessed using confirmatory factor analysis. Structural equation modeling was used to validate the proposed hypotheses between constructs.FindingsResults showed that diningscape has a second-order factor structure consisting of five dimensions, namely food and drinks, service quality, servicescape, social functions and soundscape. Diningscape positively influences customer satisfaction and word of mouth.Practical implicationsSocial function is the dominant factor of diningscape while female customers are more sensitive towards food and drinks, service quality, servicescape and soundscape. Thus, restaurants should not overcrowd their premises. Additionally, restaurants must strive to provide a wide variety of food and drinks, show service intimacy and be decorated specially with appropriate sonic environment as female customers can have a big influence on where to dine.Originality/valueThe study reveals that diningscape is multidimensional and shall be characterized in a holistic manner. Additionally, it helps restaurant managers to focus on the more important features, such as social functions, and food and drinks that customers value most.
Exploring the impact of supply chain quality management on market performance: the mediating roles of supply chain integration and operational performance
Authors: Abdallah Ayman Bahjat , Alhyari Salah , Alfar Nour A. 
Published: April 2023

PurposeThis study examines the impact of supply chain quality management (SCQM) on supply chain integration (SCI), operational performance (OP) and market performance (MP). The mediating impacts of SCI and OP on the SCQM–MP relationship are also explored and the indirect impact of SCQM on OP via SCI is investigated.Design/methodology/approachThe study population included all Jordanian manufacturing firms. Data were collected through self-administered questionnaires filled out by a randomly selected sample of 267 participants from various industries. The model of this study was assessed for validity and reliability, which were found to be satisfactory. Research hypotheses were tested using structural equation modeling (SEM) with Analysis of a Moment Structures (AMOS) software.FindingsThe findings have important implications for the direct and indirect impacts of SCQM on MP. While the direct impact was found to be insignificant, SCI and OP were found to fully mediate the SCQM–MP relationship. The findings also demonstrated that the impact of SCI on OP was insignificant; likewise, the mediating impact of SCI on SCQM–OP was insignificant.Originality/valueThis study fills a knowledge gap regarding the impact of SCQM on MP. This study is one of the first studies to explore the mediating impacts of SC and operational capabilities in terms of SCI and OP on the SCQM–MP relationship. This study is also one of the first to explore the indirect impact of SCQM on OP via SCI. This study conceptualizes SCQM as collaborative quality management (OP) practices that are externally focused to include upstream and downstream members of the SC.
Servant leadership, innovative work behavior and innovative organizational culture: the mediating role of perceived organizational support
Authors: Ekmekcioglu Emre Burak , Öner Kürşad 
Published: April 2023

PurposeThe purpose of this study is to examine the effects of servant leadership (SL) and innovative organizational culture (IOC) on employees' innovative work behavior (IWB). In addition, this paper attempts to examine the mediating role of perceived organizational support (POS) in these relationships.Design/methodology/approachData were collected from 280 employees working in technopark companies located in Turkey, which require intensive IWB. Structural equation modeling and bootstrapping procedure were used to test the hypothesized relationships.FindingsThe findings suggest that SL, and IOC are significantly and positively related to employees' IWB. The results also show that SL and IOC stimulate employees' IWB through POS.Research limitations/implicationsBecause this study was carried out by employing a cross-sectional research design with data obtained from the same source, the inferences about the causality among the variables cannot be inferred.Practical implicationsThe empirical findings suggest that organizations should make efforts to promote SL and improve IOC in order to harvest IWB from their employees. Moreover, organizations and managers need to recognize the importance of the POS by employees, and therefore form an adequate working environment, create and utilize policies and procedures accordingly.Originality/valueThis study suggests ways for organizations to enhance their innovativeness through IOC and SL applications in pursue of harvesting employees' IWB using POS by employees as mediator. This study is also original, in that no previous studies have investigated the mediating role of POS in the relationship between IOC, SL and IWB.
Which should come first? Examining diversity, equity and inclusion
Authors: Russen Michelle , Dawson Mary 
Published: April 2023

Purpose
The purpose of this critical review is to address issues with the current school of thought that diversity must come before inclusion in the diversity, equity and inclusion (DEI) process and propose an alternate solution.

Design/methodology/approach
This review takes a critical constructionist lens such that changes in social norms have morphed over time, refining the meaning and implementation of DEI initiatives in research and the workplace. This review is framed within the context of hospitality organizations.

Findings
The conflicting results in DEI research (whether DEI practices are positive or negative) are explained by diversity being the core factor. It is proposed that inclusion is the starting place and determinant of success in creating a diverse workforce. If inclusion comes first and is followed by equitable treatment, then diversity (and diverse representation) naturally follows.

Research limitations/implications
This review offers a novel perspective on the relationship between diversity, equity and inclusion, which was previously ambiguous. Research rarely includes all three as variables in the past, and does not use diversity as an outcome, but rather as a starting point.

Originality/value
This research suggests that unless an organization begins with an inclusive climate, there will be no benefit to having diverse candidates, nor will there be long-term retention of a diverse staff. It is recommended to begin with inclusion, implement equitable practices and diversity will increase through the enacted and espoused values.
Growth effect of trade misinvoicing in Sub-Saharan Africa: the role of governance
Authors: Babatunde Musibau Adetunji , Afolabi Joshua Adeyemi 
Published: May 2023

PurposeThe growing volume of trade misinvoicing in Sub-Saharan Africa (SSA) calls for serious concern, particularly given its effect on macroeconomic fundamentals. Despite the growing body of literature on the growth effect of trade misinvoicing, empirical evidence on the role of governance in moderating the effect is quite scarce, particularly for SSA. The purpose of this paper is to provide insights into the growth effect of trade misinvoicing in SSA as well as the moderating role of governance in this regard.Design/methodology/approachThe feasible generalised least square estimator was applied to analyse relevant data, spanning 2009–2018, of 35 SSA countries. Governance indicators were classified into economic, political and institutional governance, and their individual role in moderating the nexus between trade misinvoicing and economic growth was explored.FindingsThis paper showed the presence of cross-sectional dependence among SSA countries and long-run convergence of the estimated variables. The empirical finding showed that trade misinvoicing has a negative growth effect in the selected SSA countries, but both economic and political governance are crucial in lowering the observed negative growth effect.Practical implicationsTo curtail trade misinvoicing, SSA policymakers should go beyond just designing anti-money laundering policies to effectively implementing the policies for improved growth prospects. More so, the government of each SSA country must devise means of strengthening governance and building effective, accountable and transparent institutional frameworks that will constantly check and discourage trade misinvoicing activities.Originality/valueThe originality of this paper stems from its novel assessment of the role governance plays in moderating the growth effect of trade misinvoicing in SSA using the feasible generalised least square estimator. It also details the strategies needed to effectively tackle trade misinvoicing.
Understanding drivers of innovation in information technology companies using a grounded theory approach
Authors: Dani Mugdha Vinod , Gandhi Aradhana Vikas , Sharma Anshu 
Published: April 2023

PurposeInnovation is a process that enables organizations to sustain and prosper in a competitive business environment. This study aims to understand the key drivers of innovation within organizations from the lens of employees.Design/methodology/approachThis study uses a grounded theory approach based on semi-structured in-depth interviews of 20 professionals working in information technology (IT) firms. Judgmental sampling was adopted to select the respondents. Open coding, inductive analysis was conducted on the interview transcripts to generate relevant themes.FindingsEmerging themes indicate that innovation within organizations can be triggered as a response to client requirements or the employees’ inherent urge to innovate. Certain factors such as user engagement, formal organizational processes and practices, informal collaborative practices, the role played by experts and leaders and upskilling were seen to enable innovative outcomes within organizations.Practical implicationsFindings from this study will allow leaders to activate the identified drivers by designing suitable organizational processes and strategies to nurture a successful innovation culture within their teams.Originality/valueThis grounded theory-based study looks at drivers of innovation within IT organizations from the perspective of employees.