bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactate Promotes Nucleus Pulposus Cell Senescence, Oxidative Stress, and Corresponding Intervertebral Disc Degeneration
Authors: Zhang Yuyao , Liu Libangxi , Qi Yuhan , Lou Jinhui , Chen Yuxuan , Liu Chao , Li Haiyin , Chang Xian , Hu Zhilei , Li Yueyang , Zhang Yang , Feng Chencheng , Zhou Yue , Zhai Yu , Li Changqing 
Published: July 2023

Abstract
The accumulation of metabolites in the intervertebral disc is considered to be an important cause of intervertebral disc degeneration (IVDD). Lactate, which is a metabolite that is produced by cellular anaerobic glycolysis, has been proven to be closely associated with IVDD. However, little is known about the role of lactate in nucleus pulposus cell (NPC) senescence and oxidative stress. This study attempted to investigate the effect of lactate on NPC senescence and oxidative stress as well as the underlying mechanism. A puncture-induced disc degeneration (PIDD) model was established in rats. Metabolomics analysis proved that lactate levels were significantly increased in the degenerated intervertebral discs. Elimination of excessive lactate levels using lactate oxidase (LOx)-overexpressing lentivirus alleviated the progression of IVDD. In vitro experiments showed that high concentrations of lactate could induce senescence and oxidative stress in NPC. High-throughput RNA sequencing results and bioinformatic analysis demonstrated that the induction of NPC senescence and oxidative stress by lactate may be related to the PI3K/Akt signalling pathway. Further study verified that high concentrations of lactate could induce NPC senescence and oxidative stress by inhibiting PI3K/Akt signalling and the downstream Akt/p21/p27/cyclin D1 and Akt/Nrf2/HO-1 pathways. Utilizing molecular docking and microscale thermophoresis assay, we found that lactate could suppress Akt phosphoactivation by binding to the Lys39 and Leu52 residues in the PH domain of Akt. These results highlight the involvement of lactate in NPC senescence and oxidative stress, and lactate may become a novel potential therapeutic target for the treatment of IVDD.
FOXQ1 promotes pancreatic cancer cell proliferation, tumor stemness, invasion and metastasis through regulation of LDHA-mediated aerobic glycolysis
Authors: Wu Changhao , Zheng Chenglong , Chen Shiyu , He Zhiwei , Hua Hao , Sun Chengyi , Yu Chao 
Published: July 2023

Abstract
Pancreatic cancer (PC), a gastrointestinal tract malignant tumor, has a poor prognosis due to early metastases and limited response to chemotherapy. Therefore, identifying novel therapeutic approaches for PC is critical. Epithelial-mesenchymal transition (EMT) was known as the vital progress in the PC development, we constructed the EMT-related prognosis model to screen out that FOXQ1 probably involving in the EMT regulation. FOXQ1 has been linked to the malignant process in a number of cancers. However, its function in PC is unknown. In our work, the expression of FOXQ1 was elevated in PC tissues, and high level of FOXQ1 in PC was linked to patients' poor prognosis. FOXQ1 overexpression promoted aerobic glycolysis and enhanced PC cell proliferation, tumor stemness, invasion and metastasis. Whereas, FOXQ1 silencing showed the reverse effect. Furthermore, mechanistic studies indicated that FOXQ1 promotes LDHA transcription, thus modulates aerobic glycolysis to enhance PC cell proliferation, tumor stemness, invasion, and metastasis by increasing LDHA expression. Therefore, these novel data suggest that FOXQ1 may be a possible therapeutic target in PC.
Role of Some Inflammasomes in Rheumatoid Arthritis Patients in Egypt
Authors: Ibrahem Reham , Raghip Mervat A , Abdelwahed Mamdouh M , Amin Noha S , Abualfadl Esam M , Waly Nancy GFM 
Published: July 2023

Abstract
Aims: the study aims to demonstrate the role of some inflammasomes genes (NLRC4 (the NLR family, CARD domain-containing protein 4), NLRP1 (NLR family, pyrin domain containing 1), ASC (Apoptosis associated speck-like protein containing a CARD), and CASPASE-1) in the pathogenesis of Rheumatoid arthritis (RA) in Egyptian population.
Main methods: PBMCs isolation using Histopaque-1077, RNA extraction by TRI reagent, cDNA synthesis using cDNA synthesis kit, measurement of gene expression level of NLRC4, NLRP1, ASC, and CASPASE-1 in all subjects and using GAPDH gene as a reference by quantitative real-time PCR, measurement of serum level of IL-1β and IL-18 using ELISA.
Key findings: the results showed dysregulated inflammasomes expression that may participate in the pathogenesis of the inflammatory process of the disease.
Significance: revealing the role of inflammasomes in RA pathogenesis can provide a promising therapy for the treatment and management of the disease
The climate niche of Homo Sapiens
Authors: Tol Richard S.J. 
Published: July 2023

Abstract

I propose the Dominicy-Hill-Worton estimator to estimate the current climate niche of Homo Sapiens and our croplands. I use this to extrapolate the degree of unprecedentedness of future climates. Worton’s peeled hull is a non-parametric, N-dimensional generalization of order statistics. Dominicy and colleagues show that Hill’s estimator of the tail-index can be applied to any homogeneous function of multivariate order statistics. I apply the DominicyHill estimator to transects through Worton’s peels. I find a thick tail for low temperatures and a thin tail for high ones. That is, warming is more worrying than cooling. Similarly, wettening is more worrying than drying. Furthermore, temperature changes are more important than changes in precipitation. The results are not affected by income, population density, or time. I replace the Hill estimator by the QQ one and correct it for top-censoring. The qualitative results are unaffected.
JEL codes: C46, N50, O44, Q54
Psychometric Analysis of the questionnaires for the assessment of lower limbs available in Italian version: a systematic review
Authors: BARNI LUCA , Carrasco-Vega Elio , Martín-Martín Jaime , Galli Manuela , Freddolini Marco , Merchan-Baeza Jose , Gonzalez-Sanchez Manuel 
Published: July 2023

Abstract
Introduction
The present study aims to analyze the structural and psychometric properties of the questionnaires published in Italian to evaluate the lower limbs.
Material and Methods
two blinded researcher performed a literature searches on the databases: Pubmed, Scopus, Cochrane, Dialnet, Cinahl, Embase, and PEDro databases. For the Keywords was using the Boolean operators "OR" and "AND": lower limb, hip, knee, foot and ankle, questionnaire, Italian, psychometric, validity and Validation. In each of the selected articles, the structural characteristics and, secondly, the psychometric characteristics were analysed.
Results
A great heterogeneity is observed in the number of items that made up each questionnaire, ranging between 4 and 42; most of the questionnaires are completed in less than 10 min, with some exceptions. Half of the selected questionnaires (16/32) presented subcategories. The reliability of the vast majority of the questionnaires was good or excellent. The criterion validity was very heterogeneous among the questionnaires identified. The construct validity was calculated at 4/32, while the sensitivity and the Standard error measurement (SEM) were 8/32, respectively.
Conclusion
The Italian versions of the questionnaires studied to evaluate the lower limbs show good essential structural and psychometric characteristics, also comparable to versions in other languages.
Beyond the single pathway: co-expression analysis reveals distinct roles of dual carbon fixation in Riftia pachyptila symbionts
Authors: Mitchell Jessica H. , Freedman Adam H. , Delaney Jennifer , Girguis Peter R. 
Published: July 2023

Abstract

Biological carbon fixation is foundational to the biosphere. Most organisms possess one carbon fixation pathway. The hydrothermal vent tubeworm Riftia pachyptila’s chemoautotrophic symbionts, however, possess two functional pathways: the Calvin Benson-Bassham (CBB) and the reductive tricarboxylic acid (rTCA) cycles. Little is known about how Riftia’s symbionts and related organisms coordinate the functioning of these two pathways. Here we investigated net carbon fixation rates, transcriptional/metabolic responses, and transcriptional co-expression patterns of Riftia pachyptila’s endosymbionts by incubating tubeworms at environmental pressures, temperature, and geochemistry. Results showed that rTCA and CBB transcriptional patterns varied in response to different geochemical regimes and that each pathway is allied to specific metabolic processes, suggesting distinctive yet complementary roles in metabolic function. Net carbon fixation rates were also exemplary, and accordingly we propose that co-activity of CBB and rTCA may be an adaptation for maintaining high carbon fixation rates, conferring a fitness advantage in dynamic vent environments.
Disabled-2: a protein up-regulated by high molecular weight hyaluronan has both tumor promoting and tumor suppressor roles in ovarian cancer
Authors: Price Zoe K , Lokman Noor A , Sugiyama Mai , Koya Yoshihiro , Yoshihara Masato , Oehler Martin K , Kajiyama Hiroaki , Ricciardelli Carmela 
Published: July 2023

Abstract
Although the pro-tumorigenic functions of hyaluronan (HA) are well documented there is limited information on the effects and targets of different molecular weight HA. Here, we investigated the effects of 27kDa, 183kDa and 1000kDa HA on ES2 ovarian cancer cells overexpressing the stem cell associated protein, Notch3. 1000kDA HA promoted spheroid formation in ES2 cells mixed with ES-2 overexpressing Notch3 (1:3). We report disabled-2 (DAB2) as a novel protein regulated by high molecular weight HA and further investigated its role in ovarian cancer. DAB2 was downregulated in ovarian cancer compared to normal tissues but increased in metastatic ovarian tumors compared to primary tumors. High DAB2 expression was associated with poor patient outcome and positively correlated with HA synthesis enzyme HAS2, HA receptor, CD44 and EMT and macrophage markers. Stromal DAB2 immunostaining was significantly increased in matched ovarian cancer tissues at relapse compared to diagnosis and associated with reduced survival. However, DAB2 overexpression significantly reduced invasion by both A2780 and OVCAR3 cells in vivo. Our research identifies a novel relationship between HA and DAB2. Furthermore, we highlight a complex relationship of both pro-tumorigenic and tumor suppressive functions of DAB2 in ovarian cancer. Further research should explore the pro-tumorigenic role of DAB2 within the tumor microenvironment of ovarian cancer.
FuXi: A cascade machine learning forecasting system for 15-day global weather forecast
Authors: Li Hao , Chen Lei , Zhong Xiaohui , Zhang Feng , Cheng Yuan , Xu Yinghui , Qi Yuan 
Published: July 2023

Abstract
Over the past few years, due to the rapid development of machine learning (ML) models for weather forecasting, state-of-the-art ML models have shown superior performance compared to the European Centre for Medium-Range Weather Forecasts (ECMWF)'s high-resolution forecst(HRES) in 10-day forecasts at a spatial resolution of 0.25°. However, the challenge remains to perform comparably to the ECMWF ensemble mean (EM) in 15-day forecasts. Previous studies have demonstrated the importance of mitigating the accumulation of forecast errors for effective long-term forecasts. Despite numerous efforts to reduce accumulation errors, including autoregressive multi-time step loss, using a single model is found to be insufficient to achieve optimal performance in both short and long lead times. Therefore, we present FuXi, a cascaded ML weather forecasting system that provides 15-day global forecasts with a temporal resolution of 6 hours and a spatial resolution of 0.25°. FuXi is developed using 39 years of the ECMWF ERA5 reanalysis dataset. The performance evaluation, based on latitude-weighted root mean square error (RMSE) and anomaly correlation coefficient (ACC), demonstrates that FuXi has comparable forecast performance to ECMWF EM in 15-day forecasts, making FuXi the first ML-based weather forecasting system to accomplish this achievement.
Assessment of transcranial Doppler indices after MgSO4 administration in severe preeclamptic women with neurologic symptoms
Authors: Lee Sul , Lee Hyun-Joo , Yu Eun-Hee , Yoon Hyung-Joon , Jo Hyun-Been , Kim Seung-Chul 
Published: July 2023

Abstract
Purpose: To investigate the effect of intravenous MgSO4 on maternal cerebral hemodynamics as well as the association between altered Doppler indices of the ophthalmic arteries and ocular lesions in patients with preeclampsia.
Methods: After each of the 15 included patients was diagnosed with preeclampsia, MgSO4 was infused followed by transcranial Doppler tests of the indices of the ophthalmic, anterior, middle, posterior cerebral, vertebral, and basilar arteries, followed by a second MgSO4 infusion. The peak, mean, diastolic velocity, and pulsatile and resistance indices of each artery were automatically measured during testing. Based on the emergent data, the cerebral perfusion pressure, resistance-area product, and cerebral flow index were calculated.
Results: The cerebral perfusion pressure of the posterior cerebral arteries significantly decreased following the infusion of MgSO4 (p<0.05). Before the infusion of MgSO4, cerebral perfusion pressure and cerebral flow index of the ophthalmic arteries were significantly increased (p<0.05) in the preeclamptic pregnant patients with ocular lesions compared those without ocular lesions. After the infusion of MgSO4, the cerebral perfusion pressure and cerebral flow index of both ophthalmic arteries were slightly decreased, but the difference was not significant.
Conclusions: Altered Doppler indices following the infusion of MgSO4 suggest significant changes in the hemodynamics of the posterior cerebral and ophthalmic arteries that are particularly related to the neurological signs and symptoms of women with preeclampsia. These findings may improve the understanding of the mechanism of the cerebral complications of preeclampsia. Advancing comprehension of these underlying mechanisms is postulated to play a pivotal role in the mitigation of hypertensive encephalopathy associated with preeclampsia.
The first simultaneous spectroscopic and monochromatic imaging observations of short-wavelength infrared aurora of N2+ Meinel (0,0) band at 1.1 μm with incoherent scatter radar
Authors: Nishiyama Takanori , Kagitani Masato , Furutachi Senri , Iwasa Yuki , Ogawa Yasunobu , Tsuda Takuo T , Dalin Peter , Tsuchiya Fuminori , Nozawa Satonori , Sigernes Fred 
Published: July 2023

Abstract
This study presents a first simultaneous observation of N2+ Meinel (0,0) band (hereafter, N2+(M)) aurora by cutting-edge short wavelength infrared imaging spectrograph (NIRAS-2) and monochromatic camera (NIRAC) installed at Kjell Henriksen Observatory (78ºN, 16ºE). On January 21 2023, N2+(M) intensification of associated with a band-shape aurora structure was observed by the NIRAS-2 and the NIRAC by temporal resolutions of 30 seconds and 20 seconds, respectively. Additionally, the European incoherent scatter Svalbard Radar also observed electron density variations at the same time. Electron density measured at altitude ranges from 100 km 120 km changed in the same way as N2+ (M) intensity, which implies that a primary source of N2+ (M) emissions is direct collisions of N2 by precipitating electrons penetrating down to around 100 km altitude (up to 10 keV). However, the observation also demonstrated moderate correlations between N2+ (M) intensity and electron density above 140 km, which implies that different N2+(M) generation process, N2 charge exchange with O+, may work up to near 160 km and make a non-negligible contribution to N2+(M) emissions. This hypothesis would be verified with further radar observations or stereo imaging observations useful to estimate the vertical distribution of the emission layers. The N2+ (M) is a very promising target wavelength for aurora observation because the quality of sensors is highly expected to improve further and further. Continuous observations with our new instruments will undoubtedly provide an important information of N2+ (M) characteristics, for future missions of both balloon-borne and satellite-borne imaging.
Real-world data on neoadjuvant chemotherapy with dual-anti HER2 therapy in HER2 positive breast cancer
Authors: Yang Zheng-Jun , Xin Fei , Chen Zu-Jin , Yu Yue , Wang Xin , Cao Xu-Chen 
Published: July 2023

Abstract
Background
Neoadjuvant chemotherapy with dual-targeted therapy is the standard treatment for human epidermal growth factor 2 (HER2)-positive breast cancer. Although the dual-targeted therapy has significantly improved the pathological complete response (pCR) rate, further investigation is needed to identify biomarkers that predict the response to neoadjuvant therapy.
Methods
This retrospective study analyzed 353 patients with HER2-positive breast invasive ductal carcinoma. The correlation between clinicopathological factors and pCR rate was evaluated. A nomogram was constructed based on the results of the multivariate logistic regression analysis to predict the probability of pCR.
Results
The breast pCR (b-pCR) rate was 56.1% (198/353) and the total pCR (t-pCR) rate was 52.7% (186/353). Multivariate analysis identified ER status, PR status, HER2 status, Ki-67 index, and neoadjuvant chemotherapy regimens as independent indicators for both b-pCR and t-pCR. The nomogram had an area under the receiver operating characteristic curve (AUC) of 0.73 (95% CI: 0.68–0.78). According to the nomogram, the t- pCR rate was highest in the ER-PR- HER2-positive patients (131/208) and lowest in the ER + PR + HER2-positive patients (19/73). The subgroup analyses showed that there was no significant difference in pCR rate among the neoadjuvant chemotherapy regimens in ER positive, PR positive, HER2 IHC 2+, Ki67 index < 30% population. However, for ER-PR-HER2-positive patients, the neoadjuvant chemotherapy regimen has a great influence on the pCR rates.
Conclusions
Patients with ER-negative, PR-negative, HER2 3 + and high KI-67 index were more likely to achieve pCR. THP may be used as an alternative to AC-THP or TCbHP in selected HER2-positive patients.
Age differentially affects the maintenance of adaptive immune responses induced by adenoviral versus mRNA vaccines against COVID-19
Authors: Nicoli Francesco , Dallan Beatrice , Proietto Davide , De Laurentis Martina , Gallerani Eleonora , Martino Mara , Ghisellini Sara , Zurlo Amedeo , Volpato Stefano , Govoni Benedetta , Borghesi Michela , Albanese Valentina , Appay Victor , Bonnini Stefano , Llewellyn-Lacey Sian , Pacifico Salvatore , Price David , Caputo Antonella , Gavioli Riccardo 
Published: July 2023

Abstract

Adenoviral and mRNA vaccines encoding the viral spike protein have been deployed globally to contain SARS-CoV-2. Elderly individuals are particularly vulnerable to severe infection, likely reflecting age-related changes in the immune system, which can also compromise vaccine efficacy. It has nonetheless remained unclear to what extent different vaccine platforms are impacted by immunosenescence. Here, we evaluated spike-specific immune responses elicited by vaccination with two doses of BNT162b2 or ChAdOx1-S and subsequently boosted with a single dose of BNT162b2 or mRNA-1273, comparing age-stratified participants with no evidence of prior infection with SARS-CoV-2. We found that ageing profoundly affected the durability of humoral responses and further limited spike-specific CD4+ T cell immunity as a function of progressive erosion of the naive lymphocyte pool in individuals vaccinated initially with BNT162b2, such that protective immunological memory was best maintained in the elderly after primary vaccination with ChAdOx1-S and subsequent boosting with BNT162b2 or mRNA-1273.
Renal Safety of Zoledronic Acid in Patients with Osteoporosis: A Retrospective Study
Authors: Ma Yanhua , Xu Shen , Xu Zhongyue , Zhang Ying , Lu Chunyan , Chen Decai , Wang Qin 
Published: July 2023

Abstract
Introduction
This study aimed to investigate the renal safety of Zoledronic Acid (ZOL) in patients with osteoporosis (OP).
Methods
A total of 1,523 patients with primary OP who received ZOL from January 2008 to October 2020 at our hospital were retrospectively analyzed. Baseline and one-year post-infusion renal function index data were analyzed in relation to clinical characteristics and comorbidities. Furthermore, the changes in renal function between a three-year consecutive infusion and a single infusion of ZOL were compared.
Results
In our study, patients with diabetes mellitus had a significantly lower estimated glomerular filtration rate than those without after one year of ZOL infusion, and their creatinine (Cr) and cystatin C (CysC) levels were significantly higher (all P < 0.05). Patients with malignant tumors had a significantly lower estimated glomerular filtration rate and higher Cr levels than those without (all P < 0.05). CysC levels were significantly higher in patients aged 65 and older and those with hypertension than in their counterparts (all P < 0.05). No adverse renal events such as acute kidney injury were detected. There were no significant differences in renal function index between the three-year continuous infusion group and the single infusion group after one year of infusion(s) (all P > 0.05).
Conclusion
ZOL infusion shows good renal safety in OP patients. Continuous infusion of ZOL did not exert a significant impact on renal function when compared to single infusion. However, the age of 65 years or older and comorbidities such as hypertension, diabetes mellitus, or malignant tumors may represent risk factors that predict adverse renal effects of ZOL infusion.
Mini Abstract
The renal safety of Zoledronic Acid treatment in osteoporosispatients remains incompletely elucidated. We found that the infusion has good renal safety in osteoporosis patients, but careful monitoring of renal function is recommended for patients who are aged 65 years or older and those with comorbidities such as hypertension, diabetes mellitus, or malignant tumors.
The Impact of School Service Quality on Student Learning Satisfaction: A Comparative Study of Public and Private High Schools in China
Authors: Li Tianxin , Guo Tao , Qi Zhanyong 
Published: July 2023

Abstract
This research examined the link between school service quality and student learning satisfaction in Chinese public and private high schools. A comprehensive comparative analysis was executed, involving the distribution of a questionnaire to 22,588 students from 20 districts in X City, China. Utilizing structural equation modeling, the study determined that four dimensions of school service quality—reliability, responsiveness, assurance, and empathy—exerted a positive impact on student learning satisfaction across both public and private high schools. The multi-group confirmatory factor analysis verified the structural model’s consistency between public and private high schools. Nonetheless, latent mean comparisons revealed that private high school students exhibited greater satisfaction with school service quality and their learning experiences compared to their public high school counterparts. The results imply that private schools delivered superior service quality, resulting in heightened student satisfaction. This study underscores the vital role of school and instructional factors in augmenting school service quality and student learning experiences, thereby offering invaluable insights for educators, policymakers, and school administrators in the Chinese context.