bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tunable molecular editing of indoles with fluoroalkyl carbenes
Authors: Liu Shaopeng , Yang Yong , Song Qingmin , Liu Zhaohong , Lu Ying , Wang Zhanjing , Sivaguru Paramasivam , Bi Xihe 
Published: February 2023

Abstract
Building molecular complexity from simple feedstocks through peripheral and skeletal editing is central to modern organic synthesis. Nevertheless, a controllable strategy that can modify both the core skeleton and periphery of an aromatic heterocycle with a common substrate remains undeveloped, despite its potential to maximize structural diversity and applications. Here we report a fluoroalkyl carbene-initiated chemodivergent molecular editing of indoles, allowing both skeletal and peripheral editing by trapping electrophilic fluoroalkyl carbene generated in situ from fluoroalkyl N-triftosylhydrazones. A variety of fluorine-containing N-heterocyclic scaffolds could be efficiently achieved through the tunable chemoselective editing reactions at skeleton or periphery of the indole, including one-carbon insertion, C3−H gem-difluoroolefination, tandem cyclopropanation/N1−H gem-difluoroolefination, and cyclopropanation. Mechanistic experiments and computations have probed the reaction mechanism and the origins of chemo- and regioselectivity.
Environmental regulation intensity, green finance, and environmental sustainability: empirical evidence from China based on spatial metrology
Authors: DENG WENYUEYANG , Zhang Zenglian 
Published: February 2023

Abstract
Carbon emissions are closely related to human activities. China has become the largest carbon dioxide emitter in the world because of its extensive use of high-carbon energy. The price paid by the deterioration of China's environmental quality is about 3%-8% of the total GDP yearly, and environmental sustainability is declining. Therefore, it is urgent to play the role of environmental regulation and introduce green funds to lower the carbon cycle and green development. Therefore, based on the data from 30 provinces from 2004 to 2019, this paper uses a spatial econometric model to consider the dynamic impact of environmental regulation intensity and green financial development on regional environmental sustainability. The research shows that:(1) China’s local environmental sustainability shows a noticeable spatial agglomeration effect, which is significant and spatially autocorrelated and clustered. (2) Based on the national level, the improvement of environmental regulation intensity will significantly improve regional environmental sustainability, and the development of green finance will also significantly improve regional environmental sustainability. Additionally, the intensity of environmental regulation has a significant positive spatial spillover effect, which may increase environmental sustainability in neighboring provinces. The impact of green financial development on environmental sustainability also has a significant positive spatial spillover effect. (3) Based on the provincial level, environmental regulation and green financial development have a significant positive impact on the environmental sustainability of each region, with the most significant impact on the western region, the northeast and central regions in the middle, and the east coefficient being the smallest. Based on the above conclusions, this paper puts forward the "5R" model to comprehensively promote the sustainable improvement of the regional environment.
Asymptomatic Monkey Pox Virus Infection: a Self-sampling Screening Intervention Adressed to Gay, Bisexual and Other Men Who Have Sex With Men and Trans Women in Spain
Authors: Agusti Cristina , Martínez-Riveros Héctor , Hernández-Rodríguez Àgueda , Casañ Cristina , Díaz Yesika , Alonso Lucía , Martró Elisa , Muñoz-Basagoiti Jordana , Gallemí Marçal , Folch Cinta , Sönmez Ibrahim , Adell Héctor , Villar Marta , de León Alexia París , Martinez-Puchol Sandra , Pelegrin Andreu C , Perez-Zsolt Daniel , Raïch-Regué Dàlia , Mora Rubén , Villegas Luis , Clotet Bonaventura , Izquierdo-Useros Nuria , Cardona Pere-Joan , Casabona Jordi 
Published: February 2023

Abstract
We aimed to assess the prevalence of asymptomatic cases of monkeypox virus (MPXV) infection among gay, bisexual, and other men who have sex with men and trans women (TW), using a self-sampling strategy. Anal and pharyngeal swabs were tested by MPXV real-time PCR and positive samples inoculated into Vero E6 cells, which were subsequently checked for cytopathic effect (CPE).
Seven out 113 participants were MPXV positive (6.19% (95% CI: 1.75%-10.64%)). Five tested positive in pharyngeal swabs, one in anal swab and one in both. Six did not present symptoms recognized as MPXV infection. Three samples were positive for CPE, and showed anti-vaccinia pAb staining by FACS and confocal microscopy.
We describe Mpox cases that remain undiagnosed and show reproductive virus despite low viral loads and who might be able to infect others. Restricting testing to individuals reporting Mpox symptoms may not be enough to contain outbreaks.
Association rule mining with fuzzy linguistic information based on attribute partial ordered structure
Authors: Pang Kuo , Li Shaoxiong , Lu Yifan , Kang Ning , Zou Li , Lu Mingyu 
Published: February 2023

Abstract
Numerous linguistically valued facts from the actual world have been modeled using the fuzzy linguistic approaches. Data owners urgently need to mine attribute associations hidden in linguistic concept knowledge. In order to solve the problem of association rule mining with fuzzy linguistic information, this paper proposes an association rule mining approach based on fuzzy linguistic attribute partial ordered structure diagram. Firstly, complex relationships between linguistic values in association rule mining are represented by fuzzy linguistic association nodes and association paths via fuzzy linguistic attribute partial ordered structure diagram (FL-APOSD). On this basis, the maximum frequent attribute set is mined from the FL-APOSD, and then the non-redundancy association rules are extracted. Secondly, in order to show the information hidden in the rules and help users to deeply understand the mining results, a fuzzy linguistic association rule visualization approach is proposed to convert the association rules into the FL-APOSD-based knowledge representation. Finally, experimental results on real-world datasets show the proposed approach's high efficiency, outperforming two relevant state-of-the-art approaches.
HCT Frailty Scale for Younger and Older Adults Undergoing Allogeneic Hematopoietic Cell Transplantation
Authors: Kumar Rajat , Salas Maria , Atenafu Eshetu , Pasic Ivan , Bascom Ora , Wilson Leeann , Lam Wilson , Law Arjun , Chen Carol , Novitzky-Basso Igor , Kim Dennis , Gerbitz Armin , Viswabandya Auro , Michelis Fotios , Lipton Jeffrey , Mattsson Jonas , Alibhai Shabbir 
Published: February 2023

Abstract
The HCT Frailty Scale is an easy prognostic tool composed of (a) Clinical Frailty Scale; (b) Instrumental Activities of Daily Living; (c) Timed-up-and-Go test; (d) Grip Strength; (e) Self-Health Rated Questionnaire; (f) Falls tests; (g) Albumin and C-reactive protein levels. This scale was designed to classify allogeneic hematopoietic cell transplant (alloHCT) candidates into fit, pre-frail and frail groups, irrespective of age. This study evaluates the ability of this frailty classification to predict overall survival (OS) and non-relapse mortality (NRM) in adult patients of all ages, in a prospective sample of 298 patients transplanted between 2018 and 2020. At first consultation, 103 (34.6%) patients were fit, 148 (49.7%) pre-frail, and 47 (15.8%) were frail. The 2-year OS and NRM of the three groups were 82.9%, 67.4%, and 48.3% (P<0.001), and 5.4%, 19.2%, and 37.7% (P<0.001). For patients younger than 60 years (n=174), the 2-year OS and NRM of fit, pre-frail, and frail groups were 88.4%, 69,3% and 53.1% (P=0.002), and 5.8%, 22,8%, and 34.8% (P=0.005), respectively; and in patients older than 60 (n=124), OS and NRM were 75.5%, 63.8% and 41.4% (P=0.006), and 4.9%, 16.4%, and 42.1% (P=0.001). In conclusion, frailty predicted worse transplant outcomes in both younger and older adults.
Parechovirus infections in human brain organoids: host immune response and not neuro-infectivity as a correlate of neuropathology
Authors: Capendale Pamela , García-Rodríguez Inés , Mulder Lance , Depla Josse , Freeze Eline , Koen Gerrit , de Sá Renata Vieira , Calitz Carlemi , Pajkrt Dasja , Srid Adithya , Wolthers Katja 
Published: February 2023

Abstract
Parechovirus A (PeV-A) is a species in the Picornaviridae family that can cause a variety of diseases, mainly in children.The most prevalent genotype, PeV-A1, can causes mild respiratory and gastrointestinal symptoms while the second most prevalent genotype, PeV-A3 can elicit severe neurological disease such as meningoencephalitis in infants. The factors determining differential outcomes between genotypes are poorly understood. In this study, we investigated the viral dynamics and tropism of PeV-A1 and PeV-A3 infection in human induced pluripotent stem cell (hiPSC)-derived unguided neural organoids (UNOs). UNOs supported PeV-A1 and PeV-A3 replication, as measured by RT-qPCR and confirmed by TCID50. Both genotypes showed similar cell tropism and infected neurons and astrocytes. Despite replicating up to a higher titre as compared to PeV-A3, PeV-A1 infection showed no significant cytokine upregulation while PeV-A3 infection resulted in an increased production of IFN-λ1 and CXCL10. This effect was also seen for Echovirus 11, another picornaviruses resulting in neurological disease. Blocking the IFN-pathway with Ruxolitinib resulted in enhanced replication of PeV-A3 indicating IFN-mediated restriction of PeV-A3 replication. This genotype-specific immune response could explain the exacerbated PeV-A3 associated severe clinical neuropathology.
Stable Trapping of Multiple Proteins at Physiological Conditions Using Nanoscale Chambers with Macromolecular Gates
Authors: Svirelis Justas , Adali-Kaya Zeynep , Emilsson Gustav , Andersson John , Medin Jesper , Vattikunta Radhika , Hulander Mats , Kolman Krzysztof , Olsson Oliver , Sakiyama Yusuke , Lim Roderick , Dahlin Andreas 
Published: February 2023

Abstract
The possibility to detect and analyze single or few biological molecules has proven very important for understanding their function and interaction mechanisms. Ideally, the molecules should be confined to a nanoscale volume so that the observation time can be extended. However, it has proven difficult to develop reliable, non-invasive trapping techniques for biomolecules under physiological conditions. Here we present a new concept for long-term and tether-free trapping of proteins without exposing them to any field gradient forces. We show that a responsive polymer brush can make solid state nanopores switch between a fully open and a fully closed state with respect to proteins, while always allowing the passage of solvent, ions and small molecules (up to ~1 kg/mol). This makes it possible to trap a very high number of proteins (500-1000) inside nanoscale chambers with a volume as small as one attoliter, reaching concentrations up to 60 g/L, which is orders of magnitude higher than for existing container-based technologies. Additionally, our method is fully compatible with parallelization by imaging arrays of nanochambers. As an application example, we show that enzymatic cascade reactions can be performed with multiple native enzymes under full nanoscale confinement and steady supply of reactants. This platform will greatly extend the possibilities of single/few molecule analysis by optical methods, and should be particularly useful for studying protein-protein interactions, such as the dynamics of oligomerization.
The design of crosslinks in different vulcanized systems to improve crack growth resistance for carbon black/graphene oxide/ natural rubber composites
Authors: Li Zhiwei , An Dong , He Rizheng , Sun Zhijian , Li Jiaxiong , Zhang Zhiyi , Liu Yaqing , Wong Chingping 
Published: February 2023

Abstract
Herein, the carbon black/graphene oxide/natural rubber composites (CB/GO/NR) with different crosslink networks were prepared through the latex co-precipitation approach. Meanwhile, the type of crosslinks on the crack propagation resistance and fatigue life in different vulcanized systems were investigated. As a result, CB/GO/NR composites dominated by polysulfide in conventional vulcanization (CV) system exhibited the improved tear strength (71.6 KN/m) and lowest crack growth rate (64.1 nm/cycle), the developed crosslink network and polysulfide based crosslink structure in CV system were the key factors to improve the crack propagation resistance. In addition, the relationship between viscoelasticity and dynamic crack propagation behavior of CB/GO/NR was studied, and the CV system had the lowest loss compliance modulus( J "), thus more energy dissipation occurred in the crack propagation region and the crack propagation resistance of CV system was improved. Importantly, this work can provide the guideline for designing the rubber-based composites in the practical engineering applications.
Anticancer activity of lycopene in HT-29 colon cancer cell line
Authors: Ataseven Dilara , Öztürk Ayşegül , Özkaraca Mustafa , Joha Zıad 
Published: February 2023

Abstract
Purpose: An inverse association between serum lycopene levels and risk of cancers has been pointed out by many prospective and retrospective epidemiological studies which prompted more studies to be performed on animal models and cell cultures in order to test this hypothesis. The aim of the present study was to evaluate the antiproliferative and pro-apoptotic effect of lycopene on colon cancer HT-29 cell lines.
Materials and Methods: The effect of lycopene on the viability of HT-29 cell line was investigated using XTT assay. the levels of BCL-2, cleaved caspase 3, BAX, cleaved PARP and 8-oxo-dG in lycopene-treated HT-29 cells were measured using ELISA. gamma-H2AX and cytochrome C expression was assessed semi-quantitatively using immunofluorescence staining.
Results: Lycopene at doses of 10 and 20 μM produced a significant antiproliferative effect on HT-29 cells compared to the control (p < 0.05). The IC50 value of lycopene in HT-29 cells was found to be 4.382 μM for 24 hours. Lycopene (4.382 μM) significantly elevated cleaved caspase 3 (p <0.01), BAX, and cleaved PARP, 8-oxo-dG levels (p<0.05). the levels of γ-H2AX foci are significantly higher while the levels of cytochrome-C are lower (p<0.05) in lycopene treated HT-29 cells.
Conclusion: These results indicate that lycopene has an antiproliferative apoptotic and genotoxic effect on HT-29 colon cancer cell lines.
The multi-kinase inhibitor CG-806 exerts anti-cancer activity against acute myeloid leukemia by co-targeting FLT3, BTK, and Aurora kinases
Authors: Yu Guopan , Zhang Weiguo , Zhang Hongying , Ly Charlie , Basyal Mahesh , Rice William G. , Andreeff Michael 
Published: February 2023

Abstract

Background: Despite the development of several FLT3 inhibitors that have improved outcomes in patients with FLT3-mutant acute myeloid leukemias (AML), drug resistance is frequently observed, which may be associated with the activation of additional pro-survival pathways such as those regulated by BTK, aurora kinases, and potentially others in addition to acquired tyrosine kinase domains (TKD) mutations of FLT3 gene. FLT3may not always be a driver mutation.
Objective: To evaluate the anti-leukemia efficacy of the novel multi-kinase inhibitor CG-806, which targets FLT3 and other kinases, in order to circumvent drug resistance and target FLT3 wild-type (WT) cells.
Methods: The anti-leukemia activity of CG-806 was investigated by measuring apoptosis induction and analyzing cell cycle with flow cytometry in vitro, and its anti-leukemia
Results: CG-806 demonstrated superior anti-leukemia efficacy compared to commercially available FLT3 inhibitors, both in vitro and in vivo, regardless of FLT3 mutational status. The mechanism of action of CG-806 may involve its broad inhibitory profile of FLT3, BTK, and aurora kinases. InFLT3 mutant cells, CG-806 induced G1 phase blockage, while in FLT3WT cells, it resulted in G2/M arrest. Targeting FLT3 and Bcl-2 and/or Mcl-1 simultaneously resulted in a synergistic pro-apoptotic effect in FLT3mutant leukemia cells.
Conclusion: The results of this study suggest that CG-806 is a promising multi-kinase inhibitor with anti-leukemia efficacy, regardless of FLT3 mutational status. A phase 1 clinical trial of CG-806 for the treatment of AML has been initiated (NCT04477291).
Political teaching application in high vocational care courses based on machine learning systems
Authors: Zhou Zixia , Liu Yang 
Published: February 2023

Abstract
Higher vocational colleges need to use video system technology to transform teaching methods to transform ideological and political teaching into more vivid, more attractive and infectious forms, thereby effectively assisting students to actively learn. This article studies a video system based on machine learning, and has developed a type of high -vocational nursing course political teaching system. The system uses the principle of machine learning to build a learning early warning monitoring algorithm, then the performance and accuracy of the model are evaluated by using the algorithm.This article finds that the module can extract the hidden characteristics of traffic, and is not affected by the knowledge. The design of the high -vocational nursing course political teaching system is equipped with multiple modules such as video on -demand, management, interaction, and recording. Compared with the group comparison, it is very efficient to find that the high -vocational nursing course political teaching system in this article is very efficient. This article studies machine learning technology and applies it to the construction process of high -vocational nursing curriculum political teaching video systems.
Prevalence and demographic variation of cardiovascular, renal, metabolic, and mental health conditions in 12 million English primary care records
Authors: Cooper Jennifer , Nirantharakumar Krishnarajah , Crowe Francesca , Azcoaga-Lorenzo Amaya , McCowan Colin , Jackson Thomas , Acharya Aditya , Gokhale Krishna , Gunathilaka Niluka , Marshall Tom , Haroon Shamil 
Published: February 2023

Abstract
Background
Primary care electronic health records (EHR) are widely used to study long-term conditions in epidemiological and health services research. Therefore, it is important to understand how well the recorded prevalence of these conditions in EHRs, compares to other reliable sources overall, and varies by socio-demographic characteristics. We aimed to describe the prevalence and socio-demographic variation of cardiovascular, renal, and metabolic (CRM) and mental health (MH) conditions in a large, nationally representative, English primary care database and compare with prevalence estimates from other population-based studies.
Methods
This was a cross-sectional study using the Clinical Practice Research Datalink (CPRD) Aurum primary care database. We calculated prevalence of 18 conditions and used logistic regression to assess how this varied by age, sex, ethnicity, and socio-economic status. We searched the literature for population prevalence estimates from other sources for comparison with the prevalences in CPRD Aurum.
Results
Depression (16.0%, 95%CI 16.0-16.0%) and hypertension (15.3%, 95%CI 15.2-15.3%) were the most prevalent conditions among 12.4 million patients. Prevalence of most conditions increased with socio-economic deprivation and age. CRM conditions, schizophrenia and substance misuse were higher in men, whilst anxiety, depression, bipolar and eating disorders were more common in women. Cardiovascular risk factors (hypertension and diabetes) were more prevalent in black and Asian patients compared with white, but the trends in prevalence of cardiovascular diseases by ethnicity were more variable. The recorded prevalences of mental health conditions were typically twice as high in white patients compared with other ethnic groups. However, PTSD and schizophrenia were more prevalent in black patients.
The prevalence of most conditions was similar or higher in the primary care database than diagnosed disease prevalence reported in national health surveys. However, screening studies typically reported higher prevalence estimates than primary care data, especially for PTSD, bipolar disorder and eating disorders.
Conclusions
The prevalence of many clinically diagnosed conditions in primary care records closely matched that of other sources. However, we found important variations by sex and ethnicity, which may reflect true variation in prevalence or systematic differences in clinical presentation and practice. Primary care data may underrepresent the prevalence of undiagnosed conditions, particularly in mental health.
Long-term intake of Lacticaseibacillus helveticus enhances bioavailability of omega-3 fatty acids in the mouse retina
Authors:   Marie-Agn&#x00E8 BRINGER, Lapaquette Pierre , Terrat Sébastien , Proukhnitzky Lil , Martine Lucy , Grégoire Stéphane , Buteau Bénédicte , Cabaret Stéphanie , Rieu Aurélie , Bermudez-Humaran Luis G. , Gabrielle Pierre-Henry , Creuzot-Garcher Catherine , Berdeaux Olivier , Acar Niyazi 
Published: February 2023

Abstract

Omega-3 (n-3) polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA), are required for the structure and function of the retina. They could also help to prevent or delay the development of retinopathies. Given the accumulating evidence showing the role of gut microbiota in regulating retinal physiology and host lipid metabolism, we evaluated the potential of long-term dietary supplementation with the Gram-positive bacterium Lacticaseibacillus helveticus strain VEL12193 to modulate the retinal n-3 PUFA content. A set of complementary approaches was used to study the impact of such a supplementation on the gut microbiota and host lipid/fatty acid (FA) metabolism. L. helveticus-supplementation was associated with a decrease in retinal saturated FAs (SFAs) and monounsaturated FAs (MUFAs) as well as an increase in retinal n-3 and omega-6 (n-6) PUFAs. Interestingly, supplementation with L. helveticus enriched the retina in C22:5n-3 (docosapentaenoic acid, DPA), C22:6n-3 (DHA), C18:2n-6 (linoleic acid, LA) and C20:3n-6 (dihomo gamma-linolenic acid, DGLA). Long-term consumption of L. helveticus also modulated gut microbiota composition and some changes in OTUs abundance correlated with the retinal FA content. This study provides a proof of concept that targeting the gut microbiota could be an effective strategy to modulate the retinal FA content, including that of protective n-3 PUFAs, thus opening paths for the design of novel preventive and/or therapeutical strategies for retinopathies.