bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modular microscopy (ModMicro) solution for volumetric epifluorescence microscopic imaging
Authors: Archibald Robert , Bishop Harriet , Gibson Graham , Kallepalli Akhil 
Published: August 2023

Abstract
Fluorescence microscopy is a well-known technique for studying biological samples. However, on a global scale, microscopy techniques (beyond brightfield imaging) are inaccessible in some countries. Additionally, proprietary microscopy hardware is not modular and cannot be adapted for different architectures. In this research, we demonstrate a modular epifluorescence microscopy system and image analysis technique developed with the motivation of achieving high-resolution imaging using low-cost modular hardware. We compare the performance of the (1000 GBP) OpenFlexure-inspired epifluorescence ModMicro system to a proprietary epifluorescence microscope. With the epifluorescence ModMicro system, we imaged 'datacubes' of 0.54 x 0.40 x 0.005 mm (in 100 steps of 50 nm each) size. The image data is enhanced with a novel sharpening algorithm. Combined (light sources, microscope hardware and image sharpness enhancement algorithm), a complete system for epifluorescence microscopy that is globally accessible and cost-effective is presented.
Lnc-HIBADH-4 regulates autophagy-lysosome pathway in amyotrophic lateral sclerosis by targeting Cathepsin D
Authors: Huang Jingxuan , Yu Yujiao , Pang Dejiang , Li Chunyu , Wei Qianqian , Cheng Yangfan , Cui Yiyuan , Ou Ruwei , Shang Huifang 
Published: August 2023

Abstract
Amyotrophic lateral sclerosis (ALS) is the most prevalent and lethal class of severe motor neuron diseases (MND) with no efficacious treatment. The pathogenic mechanisms underlying ALS remain unclear. Nearly 90% of patients exhibit sporadic onset (sALS). Therefore, elucidating the pathophysiology of ALS is imperative. Long non-coding RNA (lncRNA) is a large class of non-coding RNAs that regulate transcription, translation and post-translational processes. LncRNAs contribute to the pathogenesis of diverse neurodegenerative disorders and hold promise as targets for interference in the realm of neurodegeneration. However, the mechanisms of which lncRNAs are involved in ALS have not been thoroughly investigated. We identified and validated a downregulated lncRNA, lnc-HIBADH-4, in ALS which correlated with disease severity and overall survival. Lnc-HIBADH-4 acted as a "molecular sponge" regulating lysosomal function through the lnc-HIBADH-4/miR-326/CTSD pathway, thereby impacting autophagy-lysosome dynamics and the levels of cell proliferation and apoptosis. Therefore, this study discovered and revealed the role of lnc-HIBADH-4 in the pathogenesis of ALS. With further research, lnc-HIBADH-4 is expected to provide a new biomarker in the diagnosis and treatment of ALS.
Unusual neurological symptoms as the first manifestations of celiac disease: a case report
Authors: Abbas Mona , Yasmeeneh Abeer Maamoun , Moumen Marwa , Alahmad Ali 
Published: August 2023

Abstract
Background: we report a patient with atypical presentation of celiac disease, she presented with neurologic manifestations without gastrointestinal symptoms.
Case presentation: a 32-year-old woman witha weakness of the lower extremities, gait imbalance, and tremor in the upper extremities progressing during the last two months with headache, she also complaints of amenorrhea five years ago with no apparent reason. She did not have gastrointestinal symptoms.
Her investigations showed that she had celiac disease and the neurological and endocrine manifestations improved with gluten-free diet within several weeks.
Conclusion: We should investigate for CD in patients with neurologic and endocrine manifestations and vitamins deficiency even if there are no gastrointestinal symptoms.
The Effect of Calcination Temperature and Particle Size on the Layered LiNi0.91Co0.06Mn0.03O2 for Lithium-ion Battery via Spray Drying Method
Authors: Park Hye-Jin , Sim Seong-Ju , Jin Bong-Soo , Kim Hyun-Soo 
Published: August 2023

Abstract
Ni-rich cathodes are one of the promising candidates for high-energy lithium-ion battery applications. Cost-effective production of Lithium-ion battery (LIB) cathode materials is significant to the electric vehicle (EV) industry. Various synthetic routes have already been established to fabricate Ni-rich LiNixCoyMnzO2 (NCM, x+y+z=1) cathodes with uniform particle size distribution and high density. However, there is a great need for new production systems due to the instability of long production cycles, and environmental issues. For continuous production of high-purity Ni-rich cathode materials, we synthesized the Ni0.91Co0.06Mn0.03CO3 precursor material by a simple spray-drying method using carbonate as a raw material. This material was calcined with LiOH·H2O (5 % excess) at 480 °C for 5 h and then sintered at a different calcining temperature for 15 h under an oxygen atmosphere to complete the cathode material preparation. We have identified the optimum calcining temperature range (at 680-950 ℃) for the Ni-rich cathode LiNi0.91Co0.06Mn0.03O2 (NCM91). As a result, NCM91 cathode material powders were obtained successfully. Furthermore, the obtained NCM91 cathode active material powders were characterized by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM) and energy dispersive spectrometer (EDS) study. The NCM91 calcined at 710 ℃ has very good electrochemical performance, having a discharge capacity of 190.63 mA h g-1 at the 1st cycle (0.1 C) and good capacity retention of 66.70 % even after 80 cycles.
Benefit of Structural Access Panels (SAP) for Post-Earthquake Structural Health Monitoring
Authors: Luo Zheng , MacRae Gregory , Xue Jianyang , Pettinga Didier 
Published: August 2023

Abstract
This paper develops a method to quantify the benefit of using structural access panels (SAPs) placed in internal partition walls (IPWs) or ceilings near the dissipative elements of structures for post-earthquake structural health monitoring (SHM) without damaging the building non-skeletal elements (NSEs). The benefit of SAPS is considered for different levels of shaking considering the possibility of (i) no likely structural damage, (ii) likely building irreparability, or (iii) building NSE damage. The methodology is developed for shaking intensity scenarios with annual shaking probabilities of exceedance ranging from 1/25 to 1/10,000. Structural demands for different scenarios are initially obtained using time history analysis. The number of scenarios was increased by additionally generated demands assuming lognormal distributions of response. Monte-Carlo analysis was used to assess the likelihood of reaching particular decision states, considering uncertainty associated with achieving these states. Probabilistic (time-based) benefits of using SAPs are also considered. Additionally, buildings with both conventional IPWs and low-damage IPWs are considered. Finally, the methodology is applied to a realistic 6 storey eccentrically braced frame (EBF) structure. In the study, the scenario-based analysis indicated that the SAPs were not effective during very small earthquake events where the post-earthquake inspection will not be triggered, or during very large events where the building is externally observed to be irreparable. Furthermore, if the NSEs equipped with SAPs sustain significant damage, SAPs were not needed for inspection. As a result, the SAPs were more beneficial when installed in low-damage NSEs, as compared to conventional NSEs, especially during moderate to high shaking levels. For the casestudy building considered, the probabilistic loss study indicates the break-even time for SAPs to be economical, considering initial installation costs, and inspection savings related to direct damage and downtime losses, was less than 2 years. The benefit-to-cost ratio of SAPs for rapid and reliable post-earthquake inspection was up to 26 over the expected building life of 50 years.
Theoretical foundations, computational methods, and practical applications of nondeterministic fuzzy abstraction
Authors: Deng Hui 
Published: August 2023

Abstract
Efficient and effective modeling of complex real-world situations characterized by uncertainty poses a substantial challenge. This study addresses this challenge by introducing a nondeterministic fuzzy abstraction approach to enhance efficiency and effectiveness in this context. The theoretical framework establishes a standardized representation of nondeterministic fuzzy Kripke structures, accompanied by definitions associated with nondeterministic fuzzy abstraction. The methods section presents an algorithm for realizing nondeterministic fuzzy abstraction, and it is well-suited for practical implementation in computer programs. The results section demonstrates the practical application of the proposed approach by modeling the process of treating a patient with ulcers in the terminal ileum. In the discussion section, a comparative study of nondeterministic fuzzy abstraction underscores its broad applicability and compatibility across various uncertainty and certainty model structures.
Natural Fracture Propagation Analysis of the
Silurian (Llandovery) Hot Shales of the Northern Arabian Plate
Authors: Sen Samil 
Published: August 2023

Abstract
The Silurian (Llandovery) hot shales of the northern Arabian Plate exhibit three main sets of fractures consisting of vertical-high angle to bedding, low angle to bedding, and bedding parallel fractures. The fractures are generally open, except for the vertical-high angle to bedding fractures and "beef" fractures. The fracture intensity ranges from low to high, and the shales are rich in clay and carbonates based on a ternary diagram analysis. The mineral-based brittleness index (MBI) values of the hot shales vary from 0.24 to 0.91, with an average of 0.59. Mechanical anisotropy analysis shows that Point Load IS(50) values range from 0.25 to 1.68 MPa in the bedding-parallel direction and from 2.05 to 2.46 MPa in the vertical to bedding direction. The natural fracture propagation of the hot shales is controlled by regional tectonic events, mechanical anisotropy, natural hydraulic overpressure driven by hydrocarbon maturation, and MBI values. The vertical-high angle to bedding fractures are believed to have been formed by the extensional tectonic activity associated with the opening of the Neotethys Ocean. The low angle to bedding and bedding parallel fractures are attributed to thrust tectonic activity of the Bitlis Nappes, mechanical anisotropy of laminations, and natural hydraulic overpressures caused by hydrocarbon maturation.
Genome sequence and assembly of the amylolytic Bacillus licheniformis T5 strain isolated from Kazakhstan soil
Authors: Mussakhmetov Arman , Kiribayeva Assel , Daniyarov Asset , Bulashev Aitbai , Kairov Ulykbek , Khassenov Bekbolat 
Published: August 2023

Abstract
Objectives:
The data presented in this study were collected with the aim of obtaining the complete genomes of specific strains of Bacillus bacteria, namely, Bacillus licheniformis T5. This strain was chosen based on its enzymatic activities, particularly amylolytic activity. In this study, nanopore sequencing technology was employed to obtain the genome sequences of this strain. It is important to note that these data represent a focused objective within a larger research context, which involves exploring the biochemical features of promising Bacilli strains and investigating the relationship between enzymatic activity, phenotypic features, and the microorganism's genome.
Data description:
In this study, the whole-genome sequence was obtained from one Bacillus strain, Bacillus licheniformis T5, isolated from soil samples in Kazakhstan. This sequence encompasses the complete genetic information of the strain, including genes, regulatory elements, and noncoding regions. The data reveal important insights into the genetic characteristics, phenotypic traits, and enzymatic activity of this Bacillusstrain.
The findings of this study have particular value to researchers interested in microbial biology, biotechnology, and antimicrobial studies. The genomic sequence offers a foundation for understanding the genetic basis of traits such as endospore formation, alkaline tolerance, temperature range for growth, nutrient utilization, and enzymatic activities. These insights can contribute to the development of novel biotechnological applications, such as the production of enzymes for industrial purposes.
Overall, this study provides valuable insights into the genetic characteristics, phenotypic traits, and enzymatic activities of the Bacillus licheniformis T5 strain. The acquired genomic sequences contribute to a better understanding of this strain and have implications for various research fields, such as microbiology, biotechnology, and antimicrobial studies.
MOF-modulated Fe3O4 Composited Au Nanoparticles for Antibacterial Wound Healing via Synergistic POD-like Nanozymatic Catalysis
Authors: Liu Chuan , Zhao Xuanping , Wang Zichao , Zhao Yingyuan , Li Ruifang , Chen Xuyang , Chen Hong , Wan Mengna , Wang Xueqin 
Published: August 2023

AbstractBacterial-induced wound infection and healing difficulty severely threat people’s health and lives nowadays. Although traditional antibiotic treatment provides an effective strategy to defeat pathogenic bacteria, prolonged use and overuse of antibiotics cause the bacterium evolution with antibiotic resistance. Herein, we report an innovative hybrid nanozyme independent of antibiotics used for antimicrobial wound healing, which is constructed with ultrasmall Au NPs via in-situ growth on metal-organic framework (MOF) stabilized Fe3O4NPs (Fe3O4@MOF@Au NPs, FMA NPs). The results showed that the fabricated hybrid nanozyme displayed synergistic peroxidase (POD)-like activities, and generated a remarkable level of hydroxyl radicals (·OH) when assisted with a low dose of H2O2. Furthermore, the results showed that the hybrid FMA nanozymes exhibited excellent biocompatibility and favourable antibacterial performances against both Gram-negativeEscherichia coli (E. coli)and Gram-positive (S. aureus) bacteria. Furthermore, animal experiments indicated that the hybrid nanozyme could availably promote wound repair employing satisfied biosafety. Therefore, this present study demonstrates that the fabricated hybrid nanozyme is a prospective alternative for bacteria infected wound healing, which has showed great potential for achieving non-poisonous wound repair healing in future clinical antimicrobial therapy.
New Explicit Solutions of Zoomeron Equation Using Optimal Indexing Approach
Authors: Ahmad Khalil , m Tehree , Naeem Muhammad 
Published: August 2023

Abstract
This paper presents a nonlinear physical model called the (2+1)-dimensional Zomeron equation, which is one of the equations that describes latent evolution. We apply the Power Index Method (PIM) to find analytical solutions of equations using function transformations with optimal indexing of one variable. After selecting index of the variable, then we adopt function transform of another variable that make sure all coefficient terms to change in new variable. PIM is a straightforward mathematical technique in which the indices of the independent variables are adjusted to our selected similarity transformations to effectively reduce the partial differential equation to an Ordinary Differential Equation (ODE). Several exact solutions of the nonlinear Zoumron equation have been built and are presented graphically for some values of the constant parameters. The proposed method has been effectively used to find new exact solutions of nonlinear PDE. Using these transforms, we can easily produce infinitesimal generators of the PDE.
The role of intraoperative pulmonary artery pressure monitoring in the postoperative management of infants with ventricular septal defect and pulmonary hypertension
Authors: Araki Haruna , Shikata Fumiaki , Torii Shinzo , Kitamura Tadashi , Mishima Toshiaki , Fukuzumi Masaomi , Fujioka Shunichiro , Horikoshi Rihito , Tamura Yoshimi , Ishiwaki Daiki , Miyaji Kagami 
Published: August 2023

Abstract
Background
The fast-track approach in pediatric cardiac surgery shortens hospital stays and reduces medical costs. This study aims to evaluate the feasibility of the fast-track approach and the role of intraoperative pulmonary artery pressure (PAP) monitoring in managing patients with preoperative pulmonary hypertension (PH) who underwent ventricular septal defect (VSD) closure.
Methods
This retrospective study included 94 infants with VSD and preoperative PH. Intraoperative systolic PAP monitoring was performed to evaluate the suitability of the fast-track approach (< 35 mmHg). Postoperative adverse events included in-hospital death, PH crisis and prolonged mechanical ventilation time, and nitric oxide (NO) administration.
Results
The fast-track approach was carried out in 35 patients (37%). One in-hospital death (1%) occurred in a non-fast-track patient due to postoperative PH crisis. Two patients (5.7%) required re-intubation after the fast-track approach due to upper airway issues. The frequency of NO inhalation and administration of pulmonary hypertensive medicines was significantly lower in the fast-track group than in the non-fast-track group (P < 0.001). Multivariate analysis revealed that body weight of < 4 kg at surgery was (P = 0.006), surgery by trainees (P = 0.002), and greater intraoperative systolic PAP (P < 0.001) were significant risk factors for postoperative adverse events.
Conclusions
Our findings showed that the fast-track approach after VSD closure in infants with preoperative PH was feasible as indicated by the low frequency of re-intubation and postoperative adverse events. Intraoperative systolic PAP measurement was useful for selecting patient to be included in the fast-track approach.
Prevalence and correlates of sleep disturbance among adolescents in the eastern seaboard of China: a cross-sectional study
Authors: Yang Haidong , Luan Lingshu , Xu Jiuli , Xu Xingran , Tang Xiaowei , Zhang Xiaobin 
Published: August 2023

Abstract
Background
Sleep disturbances are serious public health issues that warrant increased attention, especially in adolescents. The aim of this study was to investigate the prevalence and factors associated with sleep disorders among urban adolescents in China.
Methods
This study utilized an online survey to assess the demographic characteristics and mental health status of secondary school students in Lianyungang City. The Patient Health Questionnaire-9 (PHQ-9) was used to evaluate suicidal ideation and sleep disturbances in adolescents. The seven-item Generalized Anxiety Disorder (GAD-7) assessed anxiety symptoms, and the Perceived Social Support Scale (PSSS) was used to measure perceived social support.
Results
Among 3443 adolescents, the prevalence of sleep disorders were 10.8%, with significantly higher proportions of sleep disorders (13.7% VS 8.3%, χ2 = 26.559, P < 0.001) among female adolescents when compared to males. Binary regression analysis revealed that suicidal ideation (OR = 2.558, 95% CI: 1.897–3.450, P < 0.001) and anxiety symptoms (OR = 9.899, 95% CI: 7.317–13.393, P < 0.001) were risk factors for sleep disturbances, and significant other support (OR = 0.940, 95% CI: 0.894–0.989, P = 0.016) and good annual household income (OR = 0.661, 95% CI: 0.396–0.943, P = 0.026) were protective factors. Furthermore, multinomial logistic regression analysis showed that sex, suicidal ideation, anxiety symptoms, and parental relationship were associated with an elevated risk of experiencing more frequent sleep disturbances (all P < 0.05).
Conclusions
We have found that 10.8% of adolescents experience sleep disorders, and it is evident that various factors can influence healthy sleeping. These results underscore the significance of addressing these factors to enhance sleep health among this population.
A promising composite room temperature solid electrolyte via Incorporating LLZTO into Cross-linked ETPTA/PEO/SN matrix for all solid state lithium batteries
Authors: Li Bangxing , Yi Xianlin , Wu Fei , Kang Xing , Hu Xiaolin 
Published: August 2023

Abstract
Composite solid electrolyte (CSE), especially the composite room temperature solid electrolyte (CRTSE), is emerging as the promising electrolyte for all-solid-state lithium batteries (ASSLB) due to their ability to combine the desirable properties of ceramic and polymer-based electrolytes, and the room temperature operation condition. In this paper, the CRTSE with polyethylene oxide (PEO), Bis (fluorosulfonyl)imide (LiTFSI), Succinonitrile (SN), LLZTO inorganic fillers and cross-linked ethoxylated trimethylolpropane triacrylate (ETPTA) was proposed. With the help of the suppression dendrite growth of lithium via cross-linked microscopic pore structure, enhancement of the ionic conductivity via LLZTO fillers, wide electrochemical window via SN, the obtained CRTSE shown high ionic conductivity (2.12 × 10-4 S cm-1), high Li+ transfer number (tLi+=0.55), stable electrochemical window (5.0 V vs Li/Li+) at room temperature. The Li symmetrical cell can cycle over 500 hours stably with current density of 0.1 mA cm-2 and 0.5 mA cm-2 at room temperature. The full solid state LiFePO4 cell can successfully work over 200 cycles with capacity retention ratio of about 70% at room temperature.
On some conjectural series containing harmonic numbers of 3-order
Authors: Wei Chuanan , Xu Ce 
Published: August 2023

Abstract

Harmonic numbers are important in a lot of branches of number theory. By means of the derivative operator, the integral operator, and several summation and transformation formulas for hypergeometric series, we prove four series containing harmonic numbers of 3-order. Three of them are conjectures which were recently proposed by Z.-W. Sun.
Synthesis, Structural confirmation, Hirshfeld surface analysis, DFT Mechanistic and ADMET Properties of (E)-N-(4-bromobenzylidene)-3-methoxybenzohydrazide Monohydrate
Authors: Ananthi Kumar , Anandalakshmi Haridhass , Nepolraj Amaladoss , Akshaya Saravanan 
Published: August 2023

Abstract
Synthesis and structural elucidation of a novel, organic, hydrazone Schiff base compound and its structure was characterized by FT-IR, 1H, 13C NMR and mass spectroscopic analysis. The single crystals of (4-BRMBH) were grown from the DMSO. The compound (C15H13BrN2O3) crystallized at (E)-N'-(4-bromobenzylidene)-3-methoxybenzohydrazide monohydrate orthorhombic system with P212121 space group through single-crystal X-ray diffraction analysis. The compound is subjected to antimicrobial (antibacterial, antifungal) activities. Theoretical studies were performed using DFT by Gaussian 09 to develop the optimized geometry. Intermolecular interactions in the crystal structures were obtained using the Hirshfeld surface analysis. The majority contribution to the Hirshfeld surface is H×××H (39.5%) contacts. The molecular docking study were carried out by in silico method to analyse their anti-tuberculosis aspect against InhA, the enoyl acyl carrier protein reductase from Mycobacterium tuberculosis.