bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors affecting the total occlusion time in eyes with hyperopic anisometropic amblyopia
Authors: Utamura Keisuke , Wakayama Akemi , Matsumoto Fumiko , Shiraishi Yukari , Narita Ikumi , Tanabe Fumi , Kusaka Shunji 
Published: February 2023

Abstract
Purpose To investigate the factors that affected the total occlusion time (TOT) in eyes with hyperopic anisometropic amblyopia.
Methods Subjects were 58 patients (26 boys and 32 girls; age range, 3.6-9.2, average, 5.8 ± 1.3 years) with hyperopic anisometropic amblyopia. All the subjects had initially visited and completed occlusion therapy with improved visual acuity (VA) of 1.0 (0.0 logMAR equivalent) or better in the amblyopic eye at Kindai University Hospital between January 2007 and March 2017. Using the subjects’ medical records, five factors for the TOT were evaluated retrospectively: the age at treatment, the initial VA of the amblyopic eye, refraction of the amblyopic eye, anisometropic disparity, and the presence of microstrabismus.
Results The initial VA of the amblyopic eye ranged from 0.1 to 0.9 (median, 0.4). The TOT ranged from 140 to 1795 (median, 598) hours with an average daily occlusion time of 7 hours. The initial VA of the amblyopic eye and presence of microstrabismus were the influential factors for the TOT (p< 0.01). To achieve VA of 1.0 or better, patients with initial VA of ≤ 0.3 in the amblyopic eye required a longer TOT. Moreover, patients with concomitant microstrabismus required a 1.7-fold longer TOT compared to those without microstrabismus.
Conclusion Patient’s initial VA and the presence of microstrabismus are the significant factors that affect the TOT.
Labor Supply, Hourly Imbalances and Household consumption in Cameroon
Authors: Evou Jean-Pierre , Ntamack Simon Alain Song 
Published: February 2023

Abstract
This paper studies two things: first, the impact of observed
labor supply constraints on the welfare of household members and,
second, break down the different work times dysfunctions by
comparing observed hours to the hours actually desired by
individuals. Using ECAM IV data from 2014 and a discrete choice
collective labour supply model, it arrives at the following
results: (i) the unemployment and underemployment
constraint negatively impacts household consumption and thus their
material well-being; (ii) on the 25.53% of unemployed
women, nearly 25% of them are forced into unemployment or
inactivity. On the contrary, on the 2.54% of unemployed men, none
of them wishes to remain inactive or unemployed; (iii)
nearly one woman over four (26%) and one man over five (20%) are
forced into underemployment.

JEL code : I1 ; D6 ; J2 ; C25
Targeting MYH9 represses USP14-mediated NAP1L1 deubiquitination and enhances temozolomide response in Glioma
Authors: Chen Zigui , Yan Xin , Miao Changfeng , Liu Longyang , Liu Su , Xia Ying , Fang Weiyi , Zheng Dandan , Luo Qisheng 
Published: February 2023

Abstract
Myosin heavy chain 9 (MYH9) has been significantly involved in a number of diseases. Nevertheless, the function of MYH9 in glioma is unclear. The article aims to investigate the effect of MYH9 and to determine if MYH9 is also implicated in temozolomide chemoresistance of glioma. Here, we first found that MYH9 increased cell proliferation and temozolomide resistance in glioma. Mechanistic studies showed that MYH9 bound to NAP1L1, a potential tumor promoter has been reported to promote the tumor proliferation, and further inhibited ubiquitination and degradation of NAP1L1 by recruiting USP14. The up-regulated NAP1L1 increased the binding with c-Myc and further activated c-Myc, which induce the expression of CCND1/CDK4, thereby promoting temozolomide resistance and cell proliferation in glioma. In addition, we found that up-regulated MYH9 level was strongly related to patient survival and should be demonstrated as a negative factor in patients with glioma. Taken together, our results offer a new perspective on the role of MYH9 in glioma progression by regulating NAP1L1 deubiquitination, targeting MYH9 will have the possible clinical application in glioma treatment going forward.
Apigenin inhibits angiogenesis of retinal microvascular endothelial cells by regulating miR-140-5p/HDAC3- mediated PTEN/PI3K/AKT pathway
Authors: Fu Chaojun , Peng Jun , Ling Yanjun , Zhao Hongqing , Zhao Yongwang , Zhang Xiuli , Ai Min , Qinghua Peng , Yuhui Qin 
Published: February 2023

Abstract
Background
Diabetic retinopathy (DR) is the main reason of visual impairment. Apigenin has anti-angiogenic effects in a variety of diseases. Our aim was to explore the role of apigenin in DR and the mechanism involved.
Methods
High glucose (HG) induced HRMEC to establish DR model. HRMECs were treated with apigenin. Then we knocked down or overexpressed miR-140-5p and HDAC3, and added PI3K/AKT inhibitor LY294002. miR-140-5p, HDAC3 and PTEN were detected by qRT-PCR. Western blot measured HDAC3, PTEN and PI3K/AKT pathway related proteins expressions. Cell proliferation and migration were monitored by MTT, wound-healing assay and Transwell assay. Angiogenesis was detected by Tube formation assay.
Results
After HG treatment, miR-140-5p expression was repressed and miR-140-5p overexpression suppressed HG-induced HRMECs proliferation, migration and angiogenesis. Apigenin treatment significantly reversed the reduction in miR-140-5p level caused by HG treatment and repressed HG-induced HRMECs proliferation, migration and angiogenesis by elevating miR-140-5p. miR-140-5p targeted HDAC3, and overexpression of miR-140-5p could reverse the up-regulation of HDAC3 expression induced by HG treatment. HDAC3 could bind to the promoter region of PTEN and inhibit its expression, and then knocking down HDAC3 suppressed PI3K/AKT pathway via elevating PTEN level. In addition, apigenin inhibited angiogenesis in DR cell models by regulating miR-140-5p/HDAC3-mediated PTEN/PI3K/AKT pathway.
Conclusions
Apigenin inhibited angiogenesis of HG induced HRMECs by regulating miR-140-5p/HDAC3-mediated PTEN/PI3K/AKT pathway. Our study might provide new drugs and new targets for treating DR.
Interventions targeting the mental health and wellbeing of care-experienced children and young people: Systematic review and evidence map
Authors: Evans Rhiannon , Madonald Sarah , Trubey Rob , Noyes Jane , Robling Michael , Willis Simone , Boffey Maria , Wooders Charlotte , Vinnicombe Soo , Melendez-Torres G.J. 
Published: February 2023

Abstract
Background: The mental health and wellbeing of care-experienced children and young people (i.e. foster care, kinship care, residential care) is poorer than non-care-experienced populations. The Care-experienced cHildren and young people’s Interventions to improve Mental health and wEll-being outcomes Sytematic review (CHIMES) systematic review aimed to synthesise the international evidence-base for interventions targeting subjective wellbeing, mental health and suicide amongst care-experienced young people aged ≤ 25 years.
Methods: For the first stage of the review we constructed an evidence map identifying key clusters and gaps in interventions and evaluations. Studies were identified through 16 electronic databases and 22 websites, in addition to expert recommendations, citation tracking and screening of relevant systematic reviews. We charted interventions and evaluations with a summary narrative, tables and infographics.
Results: In total, 64 interventions with 124 associated study reports were eligible. The majority of study reports were from the USA (n=77). Interventions primarily target children and young people’s skills and competencies (n=9 interventions), the parental functioning and practices of carers (n=26), or a combination of the two (n=15). While theoretically under-specified, interventions are largely informed by theories of Attachment, Positive Youth Development, and Social Learning Theory. Current evaluations prioritise outcome (n=86) and process (n=50), with a paucity of study reports including theoretical descriptions (n=24) or economic evaluations (n=1). Interventions most frequently target outcomes related to mental health, neurodevelopment and behavioural outcomes, notably total social, emotional and behavioural problems (n=48 interventions) and externalizing problem behaviours (n=26). There are a limited number of interventions targeting subjective wellbeing or suicide-related outcomes.
Conclusions: Future intervention development might focus on structural-level intervention theories and components, and target outcomes related to subjective wellbeing and suicide. In accordance with current methodological guidance for intervention development and evaluation, research needs to integrate theoretical, outcome, process and economic evaluation in order to strengthen the evidence-base.
PROSPERO Registration: CRD42020177478
A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva
Authors: Wagner Kaitlyn , Fox Phil , Gordon Elizabeth , Hahn Westen , Olsen Kenzie , Markham Alex , Buglewicz Dylan , Selemenkis Platon , Lessard Avery , Goldstein Daniella , Threatt Alissa , Davis Luke , Miller-Dawson Jake , Stockett Halie , Rugh Kristin , Turner Houston , Remias Michelle , Williams Maggie , Chavez Jorge , Galindo Gabriel , Cialek Charlotte , Koch Amanda , Fout Alex , Fosdick Bailey , Broeckling Bettina , Zabel Mark 
Published: February 2023

Abstract
In response to the SARS-CoV-2 pandemic, we developed a multiplexed, paired-pool droplet digital PCR (MP4) screening assay. Key features of our assay are the use of minimally processed saliva, 8-sample paired pools, and reverse-transcription droplet digital PCR (RT-ddPCR) targeting the SARS-CoV-2 nucleocapsid gene. The limit of detection was determined to be 2 and 12 copies per µl for individual and pooled samples, respectively. Using the MP4 assay, we routinely processed over 1,000 samples a day with a 24-hour turnaround time and over the course of 17 months, screened over 250,000 saliva samples. Modeling studies showed that the efficiency of 8-sample pools was reduced with increased viral prevalence and that this could be mitigated by using 4-sample pools. We also present a strategy for, and modeling data supporting, the creation of a third paired pool as an additional strategy to employ under high viral prevalence.
Squalene monooxygenase（SQLE) protects ovarian cancer cells from ferroptosis
Authors: Zhang Rong , Zhang Ling mei , Fan Si zhe , Wang Liang liang , Wang Bei bei , He Ze lai , Wang Li hua 
Published: February 2023

Abstract
Background: Ovarian cancer is one of the three major gynecological cancers, which is closely related to squalene monooxygenase (SQLE). We aim to clarify the role of SQLE in ovarian cancer.
Methods: The expression of SQLE was detected by qRT-PCR, Western Bolt and immunohistochemistry. The association between SQLE and ferroptosis was demonstrated by TCGA, GTEx database, TMT protein sequencing, qRT-PCR, Western Bolt, immunofluorescence, ROS detection, and lipid peroxide detection. Animal experiments verified the relationship between SQLE and ferroptosis in ovarian cancer.
Results: The expression of SQLE increased in ovarian cancer tissues and cell lines. The decreased expression of SQLE caused ferroptosis of ovarian cancer cells, and enhanced the sensitivity of ovarian cancer cells to ferroptosis inducers.
Conclusion: Our study shows that SQLE is highly expressed in ovarian cancer tissues and cells, and the high expression of SQLE in ovarian cancer may promote the occurrence and development of ovarian cancer by protecting ovarian cancer cells from ferroptosis, thus enlightening new treatment methods for ovarian cancer.
Correlation between choriocapillary blood flow area and microstructural repair of macular as well as visual recovery in idiopathic macular hole
Authors: Li Jing , Li Huilin , Wang Wenjuan , Sun Bin , Cui Tong , Zhang Xiaodan , Gao Yan , zhou guohong 
Published: February 2023

Abstract
Purpose: To evaluate the changes of choroidal blood flow area (CBFA) and the absence diameter of Ellipsoid zone with idiopathic macular hole (IMH) before and after surgery and its correlation with visual function.
Patients and methods: This prospective study was conducted in Shanxi Eye Hospital from August 2019 to December 2021. Twenty-five eyes of 25 patients with unilateral IMH were investigated. The choroidal blood flow area, the choroidal thickness and the diameter of Ellipsoid zone in the macular area were measured before and after surgery in the IMH eye. Meanwhile, we further explored their relationship.
Results: After surgery, all the macular holes were anatomically recovered. Compared with baseline, the BCVA of the eyes of IMH patients was significantly improved, the logMAR BCVA of IMH patients was 0.82±0.33 before surgery and 1.65±1.19 six months after surgery, respectively. The choroidal blood flow area was 0.463±0.046 mm2 before surgery and 0.527±0.130 mm2 six months after surgery. The diameter of the ellipsoidal Zone defect was 1811.47±488.979 before surgery and 560.00±341.858 μm at 6 months after surgery, respectively. Pearson correlation analysis showed that logMAR BCVA was positively correlated with the diameter of the absence range of the ellipsoidal Zone (r=0.69, 0.75, P<0.05) before surgery, and the choroidal blood flow area before surgery was negatively correlated with the diameter of the ellipsoidal Zone defect (r=-0.493, P<0.05).
Conclusions: PPV plus ILM peeling treatment with IMH can effectively promote the closure of macular hole and recesses. The choroidal blood flow area is smaller before IMH srugery、the larger of the diameter of ellipsoidal zone and the worse of the visual function; Along with and the smaller of the diameter of ellipsoidal zone after IMH and the gradual recovery of the retinal microstructure, the logMAR BCVA of the patient is also gradually improved, and the visual function is gradually restored. The integrity of the postoperative microstructure has been determined as a predictor of the visual function recovery after IMH.
Study on Emotion Recognition Bias in Different Regional Groups
Authors: lukac Martin , Zhambulova Gulnaz , Abdiyeva Kamila , Lewis Michael 
Published: February 2023

Abstract
Human-machine communication can be substantially enhanced by the inclusion of high-quality real-time recognition of spontaneous emotional expressions. However, successful recognition of such expressions can be negatively impacted by factors such as sudden variations of lighting, or intentional obfuscation. In addition, the presentation and meaning of emotional expressions can vary significantly, depending on external factors such as the environment within which the emotions are expressed, and the culture of the expressor. As an example, an emotion recognition model trained on a regionally-specific database, collected from North America, might fail to recognize standard emotional expressions from another region, such as East Asia. To address the problem of regional and cultural bias in emotion recognition from facial expressions, we propose a meta-model that fuses multiple emotional cues and features. The proposed approach integrates macro-expression, micro-expression, action level features and image features into a multi-cues emotion model (MCAM). The results show that the proposed meta-classifier (MCAM) reduces the bias factors and achieves successful emotion recognition in most of the evaluated scenarios. Additionally, we also identified certain cues and features of the data-sets that preclude the design of the perfect unbiased classifier.
Identification of a putative novel cholivirus in the transcriptome of Gymnema sylvestre R. Br
Authors: Sidharthan V. Kavi , Vanamala Gayatri , Rajeswari V , Baranwal V. K. 
Published: February 2023

Abstract
Gymnema sylvestre is a tropical climber species that is widely used in traditional medicine since ages. In the present study, the transcriptome datasets of G. sylvestre available in public domain were screened for the presence of novel plant viral sequences and a putative novel virus tentatively named as Gymnema sylvestre virus 1 (GysV1) was identified. Coding-complete genome segments of GysV1 that are 6.35 kb (RNA1) and 3.98 kb (RNA2) long possessed a single large open reading frame coding for a polyprotein. BLASTp, sequence identity and phylogenetic analyses revealed the relatedness of GysV1 to the members of the subgenus Cholivirus (genus Sadwavirus; family Secoviridae; order Picornavirales). Based on the species demarcation criteria of the family Secoviridae, GysV1 can be regarded as a new cholivirus member.
The Effect of Educational Intervention based on Pender’s Health Promotion Model on Calcium Consumption among Pregnant Women
Authors: Amiri Masoumeh , Heidari Neda , Rajati Mojgan , Mahaki Behzad , Rajati Fatemeh 
Published: February 2023

Abstract
Background and objective: The consumption of calcium-rich foods among pregnant women has always been one of the challenging issues of the health care system. The present study aimed to evaluate the effect of educational intervention based on Pender’s HPM on the calcium intake of pregnant women.
Materials and method: The pregnant women at three to five months were recruited using convenient sampling method and randomly assigned either to the control (n=37) or intervention (n=36) groups referred to the health centers in Kermanshah, Iran in 2019, 2020. Interventions were implemented based on the structures of Pender’s HPM with three assessments at baseline, immediately after the intervention, and after a one-month follow-up. The food frequency questionnaire was completed before and after the intervention. Data were analyzed in SPSS software version 25.
Results: There is no significant difference between the intervention and control groups at baseline (P<0.05). According to the independent sample t-test, all constructs of the Pender’s HPM except for interpersonal influences were significantly improved in the intervention rather than control groups (P>0.05).
The repeated measure ANOVA demonstrated a significant difference in the effect of the intervention on the constructs of the knowledge (F=9.40; P-value=0.001), perceived benefits (F=17.24 ; P-value=0.001), perceived barriers (F=40.80 ; P-value=0.001), perceived self-efficacy (F=10.90; P-value=0.001), activity-related affect (F= 14.85; P-value=0.001), interpersonal influences (F=21.51 ; P-value=0.001), commitment to a plan of action (F=20.20 ; P-value=0.001), and immediate competing demands and preferences (F=9.4; P-value=0.001) between the intervention and control groups. However, a significant difference was not observed in the construct of situational influences (F=1.20; P-value=0.296). The ANOVA demonstrated that the calcium consumption significantly increased in the intervention group (P<0.001).
Conclusion: Educational intervention based on the HPMchanged the nutritional behavior of calcium-rich food consumption among pregnant women. The use of Pender's HPM is recommended to improve nutritional behaviors. A theory-based educational intervention in the health care system can fill the gap in the successful implementation of nutrition education programs.
Unravelling the effect of winter holiday celebrations on SARS-CoV-2 transmission
Authors: Geenen Caspar , Thibaut Jonathan , Laenen Lies , Raymenants Joren , Cuypers Lize , Maes Piet , Dellicour Simon , André Emmanuel 
Published: February 2023

Abstract
Public holidays have been associated with SARS-CoV-2 incidence surges, although a firm causal link remains to be established. This association is sometimes attributed to events where transmissions occur at a disproportionately high rate, known as superspreading events. Here, we describe a sudden surge in new cases with the Omicron BA.1 strain amongst higher education students in Belgium. Contact tracers classed most of these cases as likely or possibly infected on New Year's Eve, indicating a direct trigger by New Year celebrations. Using a combination of contact tracing and phylogenetic data, we show the limited role of superspreading events in this surge. Finally, the numerous simultaneous transmissions allowed a unique opportunity to determine the distribution of incubation periods of the Omicron strain. Overall, our results indicate that, even under social restrictions, a surge in transmissibility of SARS-CoV-2 can occur when holiday celebrations result in small social gatherings attended simultaneously and communitywide.
Clinical value of using heart rate variability biofeedback before elective CT coronary angiography to reduce heart rate and the need for beta-blockers
Authors: Patrick Langguth , Carmen Wolf , Sam Sedaghat , Johanne Frank , Marcus Both , Olav Jansen , Mona Salehi Ravesh , Annett Lebenatus 
Published: February 2023

Abstract
Objectives: The value of biofeedback before elective coronary computed tomography angiography (CCTA) to reduce patients’ heart rates (HR) was investigated in the current work.
Methods: 60 patients who received CCTA to exclude coronary artery disease were included in our study and separated into two groups: with biofeedback (W-BF) and without biofeedback (WO-BF). The W-BF group used a biofeedback device for 15 min before CCTA. HR was determined in each patient at five measurement time points (MTP): during the pre-examination interview (MTP1), positioning on the CT patient table (MTP2), immediately before CCTA (MTP3), CCTA image acquisition (MTP4), and after completing CCTA (MTP5). If necessary, beta-blockers were administered in both groups after MTP3 until a HR of less than 65 bpm was achieved. Two board-certified radiologists subsequently assessed the image quality and analyzed the findings.
Results: Overall, the need for beta-blockers was significantly lower in patients in W-BF group than in WO-BF group (p=0.032). In patients with a HR of 81-90, beta-blockers were not required in 4 of 6 cases in the W-BF group, whereas in the WO-BF group all patients needed beta-blockers (p=0.03). We found a significant decrease in HR between MTP1 and MTP2 in W-BF as compared to WO-BF. There was no significant difference between the W-BF and WO-BF groups regarding image quality (p=0.179).
Conclusion: By using biofeedback prior to elective CCTA beta-blocker use can be significantly decreased due to effective reduction of HR, especially in patients with a HR above 80 bpm, without compromising CT image quality and analysis.
Experimental evidence of climate change extinction risk in tropical plants.
Authors: Hollenbeck Emily , Sax Dov 
Published: February 2023

Abstract

Climate change is conjectured to endanger tropical species, particularly in biodiverse montane regions, but accurate estimates of extinction risk are limited by a lack of empirical data describing tropical species’ sensitivity to climate. To fill this gap, studies must match high-quality distribution data with multi-year, transplant experiments. Here, we conducted field surveys of epiphyte distributions on three mountains in Central America and performed reciprocal transplant experiments on one mountain across sites that varied in elevation, temperature and aridity. We find that most species are unable to survive outside of their narrow elevational distributions. Additionally, our findings suggest starkly different outcomes by 2100 under different emission and temperature change scenarios. Under low-emission scenarios, most tropical montane species will survive, but under mid or high-emission scenarios a minimum of 5–36% of our study species may go extinct and 10–50% of populations may be lost. This result demonstrates that current actions towards climate mitigation and emissions reduction may significantly impact the number of tropical extinctions that will occur due to climate change. Unique in its precise test of tropical species’ climate tolerances in a large field experiment, our work corroborates earlier conjecture about wide-spread extinctions from climate change in tropical montane ecosystems.