bluuebunny/crossref_metadata_2025_split huggingface dataset:
A potential biocontrol agent Streptomyces tauricus XF for managing wheat stripe rust
Authors: Jia Ruimin , Xiao Keyu , Yu Ligang , Chen Jing , Hu Lifang , Wang Yang 
Published: April 2023

AbstractWheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease threatening global wheat production. Biocontrol by beneficial microorganisms is considered an alternative to synthetic fungicide applications. This study aimed to investigate the mechanisms involved in the biocontrol of wheat stripe rust by streptomycetes. A streptomycete strain XF, isolated from the rhizospheric soil of peony, was identified as Streptomyces tauricus based on morphological characteristics and phylogenetic analysis. We determined the inhibitory effect of XF on Pst and biocontrol effect on the disease using XF fermentation filtrate (FL) and actinomycete cell suspension (AC). Results revealed that FL inhibited urediniospore germination by up to 99% and rendered a lethality rate of 61.47% against urediniospores. Additionally, crude extract of ethyl acetate phase of FL caused cytoplasm releases from urediniospores and the deformation of germ tubes. Furthermore, histochemical analyses revealed that treatments of plants with AC and FL increased reactive oxygen species, inhibited haustorium formation, and reduced the biomass of Pst in leaves. Electron microscopy showed that XF mycelium was able to colonize the leaf surface. Moreover, gene expression assays revealed that AC and FL treatments induced the expression of a number of pathogenesis-related genes in wheat leaves. Besides, in the greenhouse experiments, the control effects of AC and FL reached 65.48% and 68.25%, respectively. In the field, application of XF fermentation broth significantly reduced the disease indices of stripe rust by 53.83%. These findings suggest that XF is a potential biocontrol agent for managing wheat stripe rust disease.
Agaricus subrufescens fermented rye affects the development of intestinal microbiota, local intestinal and innate immunity in suckling-to-nursery pigs
Authors: Wen Caifang , Geervliet Mirelle , de Vries Hugo , Fabà Lluís , den Hil Petra J. Roubos-van , Skovgaard Kerstin , Savelkoul Huub F. J. , Schols Henk A. , Wells Jerry M. , Tijhaar Edwin , Smidt Hauke 
Published: April 2023

AbstractBackgroundAgaricus subrufescensis considered as one of the most important culinary-medicinal mushrooms around the world. It has been widely suggested to be used for the development of functional food ingredients to promote human health ascribed to the various properties (e.g., anti-inflammatory, antioxidant, and immunomodulatory activities). In this context, the interest inA. subrufescensbased feed ingredients as alternatives for antibiotics has also been fuelled during an era of reduced/banned antibiotics use. This study aimed to investigate the effects of a fermented feed additive -rye overgrown with mycelium (ROM) ofA. subrufescens—on pig intestinal microbiota, mucosal gene expression and local and systemic immunity during early life. Piglets received ROM or a tap water placebo (Ctrl) perorally every other day from day 2 after birth until 2 weeks post-weaning. Eight animals per treatment were euthanized and dissected on days 27, 44 and 70.ResultsThe results showed ROM piglets had a lower inter-individual variation of faecal microbiota composition before weaning and a lower relative abundance of proteobacterial genera in jejunum (UndibacteriumandSolobacterium)and caecum (IntestinibacterandSuccinivibrionaceae_UCG_001) on day 70, as compared to Ctrl piglets. ROM supplementation also influenced gut mucosal gene expression in both ileum and caecum on day 44. In ileum, ROM pigs showed increased expression of TJP1/ZO1 but decreased expression of CLDN3, CLDN5 and MUC2 than Ctrl pigs. Genes involved in TLR signalling (e.g., TICAM2, IRAK4 and LY96) were more expressed but MYD88 and TOLLIP were less expressed in ROM pigs than Ctrl animals. NOS2 and HIF1A involved in redox signalling were either decreased or increased in ROM pigs, respectively. In caecum, differentially expressed genes between two groups were mainly shown as increased expression (e.g., MUC2, PDGFRB, TOLLIP, TNFAIP3 and MYD88) in ROM pigs. Moreover, ROM animals showed higher NK cell activation in blood and enhanced IL-10 production in ex vivo stimulated MLN cells before weaning.ConclusionsCollectively, these results suggest that ROM supplementation in early life modulates gut microbiota and (local) immune system development. Consequently, ROM supplementation may contribute to improving health of pigs during the weaning transition period and reducing antibiotics use.
The promising guide to LC–MS analysis and cholinesterase activity of Luffa cylindrica (L.) fruit using in vitro and in-silico analyses
Authors: Singh S. Ankul , Vellapandian Chitra 
Published: April 2023

Abstract
Background
Luffa cylindrica (L.) is an annual climbing plant that produces fibrous fruit and can also be used as a vegetable in northern parts of India. Various studies have been carried out on the plant and found to have anti-inflammatory, antifungal, analgesic, anti-myocardial, anti-hyper triglyceride, immunostimulant, anti-allergic, and other properties. The ethanolic extract of the Luffa cylindrica (L.) fruit has not yet been subjected to LC–MS analysis for several bioactive chemicals that target neurological diseases. Oxidative stress is an inevitable situation in AD mechanisms and is a key bridge connecting various AD pathways.

Results
Luffa cylindrica contains various phytochemicals and showed highest alkaloid content of 21.39 ± 1.47 mg of AE/g. A total of 80 compounds were identified in the ethanolic extract from LC–MS analysis. The bioactive compounds were screened for eligibility by Lipinski's rule of five for docking with receptors responsible for causing oxidative stress-associated Alzheimer's disease. Perlolyrine was chosen to perform in-silico docking. An in vitro activity of cholinesterase showed highest inhibition at 500 µg/ml. In-silico docking of perlolyrine showed better binding affinity and score. Results revealed that out of 10 docked receptors, amyloid beta showed the highest binding affinity with an energy of − 46.1 kcal/mol showing promising drug for Alzheimer's disease.

Conclusion
Based on current findings, the study reports the presence of a promising, bioactive compound (perlolyrine) and in turn provides an optimistic note in exploring its biological activity in vivo with oxidative stress-related Alzheimer's disease.
Statistical analysis of crash data and impact of COVID-19 on road crashes in Maharashtra, India
Authors: Mhetre Krantikumar V. , Thube Aruna D. 
Published: April 2023

Abstract
This study analyzes crash data from 2016 to 2020 on a National Highway in Maharashtra, India. The impact of the COVID-19 lockdown on the road crashes of the study area is presented, and recommendations to improve road safety are proposed. The crash data is collected from the “National Highways Authority of India, Kolhapur” from 2016 to 2020, and the information is classified into three scenarios: Before Lockdown, After Lockdown, and Strict Lockdown. The crash data is analyzed under three scenarios for seven different classifications followed by their sub-classifications. The time-wise analysis of crash data is performed in four-time slots, namely 00:00–05:59 AM, 06:00–11:59 AM, 12:00–17:59 PM, and 18:00–23:59 PM. The season-wise analysis of crash data is performed in three seasons: Summer, Monsoon, and Winter.The crashes that occurred on 2-lane-straight roads having T-junction are more than 90% in all three scenarios. The significant factors responsible for crashes are “Head-on collision,” “Vehicle out of control,” and “Overspeeding.” Most crashes (more than 36%) occurred between 12:00 and 17:59 PM and in the Summer season (more than 42%) in all three scenarios. The crashes in the COVID-19 “Strict Lockdown” scenario witnessed a fall of 254.55% compared to 2019 and 2018. Surprisingly, there was a rise of 137.5% and a fall of 127.27% in crashes of the COVID-19 2020 “Strict Lockdown” scenario, compared to 2017 and 2016, respectively. The crashes under the sub-classifications “Right angle collision” and “Fatal” increased in 2020 compared to the previous 4 years due to the impact of COVID-19.
The relationship between blood–brain barrier dysfunction and neurocognitive impairments in first-episode psychosis: findings from a retrospective chart analysis
Authors: Maurus Isabel , Wagner Sarah , Campana Mattia , Roell Lukas , Strauss Johanna , Fernando Piyumi , Muenz Susanne , Eichhorn Peter , Schmitt Andrea , Karch Susanne , Pogarell Oliver , Engel Rolf R. , Falkai Peter , Hasan Alkomiet , Wagner Elias 
Published: April 2023

Background
Even before the onset of psychotic symptoms, individuals with schizophrenia display cognitive impairments. Simultaneously, increasing amounts of individuals exhibit dysfunction of the blood–brain barrier (BBB). However, the impact of BBB dysfunction on neurocognitive impairment in people with first-episode psychosis has not yet been investigated.

Aims
To advance understanding of said relationship, we considered one of the largest first-episode psychosis cohorts with cerebrospinal fluid parameters available, and investigated whether BBB dysfunction is related to working memory, working speed and attention.

Method
We conducted a retrospective chart review of 121 in-patients diagnosed with a first episode of a schizophrenia spectrum disorder. Patients underwent neurocognitive testing and a lumbar puncture within routine clinical care. To define BBB dysfunction, albumin cerebrospinal fluid/serum quotients, immunoglobulin G ratios and oligoclonal band types were evaluated, and gender-specific differences investigated. Neurocognitive functioning was assessed by the Wechsler Adult Intelligence Scale, Test of Attentional Performance and Repeatable Battery for the Assessment of Neuropsychological Status. We performed simple and multiple linear regression analyses to interpret associations of interest.

Results
Of those tested, 16% showed an alteration in albumin quotients and 12% had an oligoclonal band type indicating BBB dysfunction. Notably, male patients were more likely to have an increased albumin quotient and a higher immunoglobulin G ratio than female patients. We found no significant association between BBB dysfunction and neurocognitive assessments.

Conclusions
The hypothesised relationship between BBB and neurocognitive impairments was not detectable in our retrospective cohort. Further cerebrospinal fluid-based studies with a longitudinal assessment of cognitive functioning and disease trajectory are urgently needed.
Accessing psychological therapies following self-harm: qualitative survey of patient experiences and views on improving practice
Authors: Quinlivan Leah , Gorman Louise , Monaghan Elizabeth , Asmal Sadika , Webb Roger T. , Kapur Nav 
Published: April 2023

Background
Psychological therapies following an episode of self-harm should happen quickly to ensure patients receive the care they need and to reduce the likelihood of repetition.

Aims
We sought to explore patients' subjective experience of accessing psychological therapies following self-harm and their views on improving practice.

Method
Between March and November 2019, we recruited 128 patients and 23 carers aged 18 years or over from 16 English mental health trusts, from community organisations and via social media. Thematic analyses were used to interpret the data.

Results
Participants reported long waiting times, multiple failed promises and rejection when trying to access psychological therapies following self-harm. Poor communication and information provision contributed to uncertainty, worsening mental health and further self-harm. Other barriers included: lack of tailored interventions, stigmatising responses, use of exclusionary thresholds to access services, and punitive approaches to treating these patients. Participant recommendations to improve access to psychological therapies included: (a) the importance of compassionate and informed staff; (b) having timely access to aftercare from well-funded and well-resourced teams; (c) continuity of care, improved communication, and support during waiting times and while navigating the referral process; (d) greater information on the availability and benefits of psychological therapies; and (e) greater choice and flexibility over interventions.

Conclusion
Our findings identify long waiting times and inadequate service provision as barriers to high-quality and safe aftercare for patients who have self-harmed. Consistent with clinical guidelines, all patients should receive prompt aftercare and access to tailored psychological treatments following a self-harm episode.
Precision Oncology Core Data Model to Support Clinical Genomics Decision Making
Authors: Botsis Taxiarchis , Murray Joseph C. , Ghanem Paola , Balan Archana , Kernagis Alexander , Hardart Kent , He Ting , Spiker Jonathan , Kreimeyer Kory , Tao Jessica , Baras Alexander S. , Yegnasubramanian Srinivasan , Canzoniero Jenna , Anagnostou Valsamo ,   The Johns Hopkins Molecular Tumor Board Investigators, Pratilas Christine , Xian Rena R. , Gocke Christopher D. , Lin Ming-Tseh , Halper-Stromberg Eitan , Zou Ying , Olayinka-Kamson David , Schreck Karisa , Grossman Stuart , Fiallos Katie , Petry Dana , Visvanathan Kala , Wolff Antonio , Santa-Maria Cesar , Nunez Raquel , Meyer Christian , Laterra John , Sterns Vered , Smith Karen L. , Armstrong Deborah , Karchin Rachel , Karaindrou Katerina , Zandi Lily , Majcherska-Agrawal Marta , Too Faith , Dobs Adrian S. , Moore Joan , Pindzola Ander , Akunyili Ikechukwu , Fatteh Maria , Lehman Jennifer 
Published: April 2023

PURPOSE Precision oncology mandates developing standardized common data models (CDMs) to facilitate analyses and enable clinical decision making. Expert-opinion–based precision oncology initiatives are epitomized in Molecular Tumor Boards (MTBs), which process large volumes of clinical-genomic data to match genotypes with molecularly guided therapies. METHODS We used the Johns Hopkins University MTB as a use case and developed a precision oncology core data model (Precision-DM) to capture key clinical-genomic data elements. We leveraged existing CDMs, building upon the Minimal Common Oncology Data Elements model (mCODE). Our model was defined as a set of profiles with multiple data elements, focusing on next-generation sequencing and variant annotations. Most elements were mapped to terminologies or code sets and the Fast Healthcare Interoperability Resources (FHIR). We subsequently compared our Precision-DM with existing CDMs, including the National Cancer Institute's Genomic Data Commons (NCI GDC), mCODE, OSIRIS, the clinical Genome Data Model (cGDM), and the genomic CDM (gCDM). RESULTS Precision-DM contained 16 profiles and 355 data elements. 39% of the elements derived values from selected terminologies or code sets, and 61% were mapped to FHIR. Although we used most elements contained in mCODE, we significantly expanded the profiles to include genomic annotations, resulting in a partial overlap of 50.7% between our core model and mCODE. Limited overlap was noted between Precision-DM and OSIRIS (33.2%), NCI GDC (21.4%), cGDM (9.3%), and gCDM (7.9%). Precision-DM covered most of the mCODE elements (87.7%), with less coverage for OSIRIS (35.8%), NCI GDC (11%), cGDM (26%) and gCDM (33.3%). CONCLUSION Precision-DM supports clinical-genomic data standardization to support the MTB use case and may allow for harmonized data pulls across health care systems, academic institutions, and community medical centers.
Association of the COVID-19 Pandemic and Changes in Patterns of Cancer-Related Mortality in the United States
Authors: Zhao Jingxuan , Han Xuesong , Miller Kimberly D. , Zheng Zhiyuan , Nogueira Leticia , Islami Farhad , Jemal Ahmedin , Yabroff K. Robin 
Published: April 2023

PURPOSE This study examined changes in patterns of cancer-related deaths during the first year of the coronavirus disease 2019 pandemic in the United States. METHODS We identified cancer-related deaths, defined as deaths attributable to cancer as the primary cause (underlying cause) or deaths with cancer documented as one of the multiple contributing factors (contributing cause) from the Multiple Cause of Death database (2015-2020). We compared age-standardized cancer-related annual and monthly mortality rates for January-December 2020 (first pandemic year) to January-December 2015-2019 (prepandemic) overall and stratified by sex, race/ethnicity, urban rural residence, and place of death. RESULTS We found that the death rate (per 100,000 person-years) with cancer as the underlying cause was lower in 2020 compared with 2019 (144.1 v 146.2), continuing the past trend observed in 2015-2019. By contrast, the death rate with cancer as a contributing cause was higher in 2020 than in 2019 (164.1 v 162.0), reversing the continuously decreasing trend from 2015 to 2019. We projected 19,703 more deaths with cancer as a contributing cause than expected on the basis of historical trends. Mirroring pandemic peaks, the monthly death rates with cancer as a contributing cause first increased in April 2020 (rate ratio [RR], 1.03; 95% CI, 1.02 to 1.04), subsequently declined in May and June 2020, and then increased again each month from July through December 2020 compared with 2019, with the highest RR in December (RR, 1.07; 95% CI, 1.06 to 1.08). CONCLUSION Death rates with cancer as the underlying cause continued to decrease in 2020 despite the increase in death rates with cancer as a contributing cause in 2020. Ongoing monitoring of long-term cancer-related mortality trends is warranted to assess effects of delays in cancer diagnosis and receipt of care during the pandemic.
Path integral solutions for Klein-Gordon particle with position-dependent mass in deformed Hulthén potential
Authors: Sadoun M. A. 
Published: April 2023

Abstract
The Green's function for a Klein-Gordon particle with position-dependent mass under the action of vector plus scalar q-deformed Hulthén potentials is evaluated by exact path integration. From the singularities of the latter Green's function, the bound states are extracted. For

, the analytic expression of the energy spectrum and the normalized wave functions for the l states are obtained within the framework of an approximation to the centrifugal term. Particular cases of these potentials are also discussed briefly and it is found that the obtained results are in good agreement with those obtained in the literature.
Diagnosis and Management of Cerebral Small Vessel Disease
Authors: Sharrief Anjail 
Published: April 2023

ABSTRACT

OBJECTIVE
Cerebral small vessel disease (CSVD) is a common neurologic condition that contributes to considerable mortality and disability because of its impact on ischemic and hemorrhagic stroke risk and dementia. While attributes of the disease have been recognized for over two centuries, gaps in knowledge remain related to its prevention and management. The purpose of this review is to provide an overview of the current state of knowledge for CSVD.

LATEST DEVELOPMENTS
CSVD can be recognized by well-defined radiographic criteria, but the pathogenic mechanism behind the disease is unclear. Hypertension control remains the best-known strategy for stroke prevention in patients with CSVD, and recent guidelines provide a long-term blood pressure target of less than 130/80 mm Hg for patients with ischemic and hemorrhagic stroke, including those with stroke related to CSVD. Cerebral amyloid angiopathy is the second leading cause of intracerebral hemorrhage and may be increasingly recognized because of newer, more sensitive imaging modalities. Transient focal neurologic episodes is a relatively new term used to describe “amyloid spells.” Guidance on distinguishing these events from seizures and transient ischemic attacks has been published.

ESSENTIAL POINTS
CSVD is prevalent and will likely be encountered by all neurologists in clinical practice. It is important for neurologists to be able to recognize CSVD, both radiographically and clinically, and to counsel patients on the prevention of disease progression. Blood pressure control is especially relevant, and strategies are needed to improve blood pressure control for primary and secondary stroke prevention in patients with CSVD.
Cervical Artery Dissection
Authors: Salehi Omran Setareh 
Published: April 2023

ABSTRACT

OBJECTIVE
Cervical artery dissection is a common cause of stroke in young adults. This article reviews the pathophysiology, etiology and risk factors, evaluation, management, and outcomes of spontaneous cervical artery dissection.

LATEST DEVELOPMENTS
Cervical artery dissection is believed to be a multifactorial disease, with environmental factors serving as possible triggers in patients who have a genetic predisposition to dissection formation. Cervical artery dissection can cause local symptoms or ischemic events, such as ischemic stroke or transient ischemic attack. Neuroimaging is used to confirm the diagnosis; classic findings include a long tapered arterial stenosis or occlusion, dissecting aneurysm, intimal flap, double lumen, or intramural hematoma. Patients with cervical artery dissection who present with an acute ischemic stroke should be evaluated for IV thrombolysis, endovascular therapy eligibility, or both. Antithrombotic therapy with either anticoagulation or antiplatelet treatment is used to prevent stroke from cervical artery dissection. The risk of recurrent ischemia appears low and is mostly limited to the first two weeks after symptom onset.

ESSENTIAL POINTS
Cervical artery dissection is a known cause of ischemic strokes. Current data show no difference between the benefits and risks of anticoagulation versus antiplatelet therapy in the acute phase of symptomatic extracranial cervical artery dissection, thereby supporting the recommendation that clinicians can prescribe either treatment. Further research is warranted to better understand the pathophysiology and long-term outcomes of cervical artery dissection.
The Transformation of Documenting and Coding for Neurologic Hospital Inpatient and Observation Services
Authors: Villanueva Raissa , Busis Neil A. , Cohen Bruce H. , Ciccarelli Luana 
Published: April 2023

ABSTRACT
Landmark changes to documenting and coding for office or other outpatient evaluation and management (E/M) codes were implemented on January 1, 2021. To decrease clinicians’ administrative burden, many documentation requirements were eliminated. In addition, major changes were made in how medical decision making and time spent on the date of the encounter are used to determine the level of service. On January 1, 2023, these changes were extended to inpatient and observation E/M services. The level of service in both inpatient and outpatient settings can now be selected based on the total time dedicated to the patient’s care on the day of the encounter or the new method of medical decision making. This article discusses the optimal ways to document and code for inpatient hospital and observation encounters after January 1, 2023.