bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient satisfaction with nursing care in Iran: a systematic review and meta-analysis
Authors: Hosseini Seyed Javad , Sadeghi Tahereh , Heydari Abbas 
Published: September 2023

Introduction:Patient satisfaction (PS) with nursing care is considered one of the most important predictors of satisfaction with hospital services. The current research was conducted to determine the level of PS with nursing care provided in hospitals in Iran.Methods:A comprehensive systematic search was conducted in various international electronic databases, such as PubMed, Scopus, Web of Science, and Persian electronic databases such as Iranmedex, and the Scientific Information Database (SID) using keywords extracted from Medical Subject Headings such as ‘Patient satisfaction’, ‘Satisfaction’, ‘Nursing care’, and ‘Nurse’ from the earliest to 27 January 2023. The risk of bias was assessed using the Joanna Briggs Institute (JBI) scale, and the analysis was performed in Stata software version 14. Subgroup and sensitivity analyses were performed to clarify the source of heterogeneity.Results:The results showed that the overall PS with nursing care in different hospitals was 0.83 (95% CI: 0.79–0.86). The proportions of complete satisfaction, partial satisfaction, and dissatisfaction were 0.38 (95% CI: 0.23–0.53), 0.45 (95% CI: 0.34–0.55), and 0.17 (95% CI: 0.12–0.21), respectively.Conclusion:However, overall satisfaction was seen in four out of five patients admitted to hospitals in Iran, complete satisfaction with nursing care was 38%. Future studies should identify the effective factors related to PS with nursing care.
Addressing Opportunities and Barriers in Telehealth Neurologic Physical Therapy: Strategies to Advance Practice
Authors: Perron Aimee E. , Garg Hina , Gallagher Sarah , Kennedy Brittany , Oxborough Sara , Schultz Emily , Thielman Gregory , Zhang Qing 
Published: September 2023

Background and Purpose:
Since the COVID-19 pandemic, the use and implementation of telehealth has expanded, with implementation moving ahead of best practice recommendations due to necessity. Telehealth has improved access and care coordination for patients with various neurologic conditions; however, information regarding therapeutic intensity, safety, and appropriateness is lacking. In 2021, the Academy of Neurologic Physical Therapy formed a Telehealth Taskforce to provide clinical and educational resources for its members and the neurologic physical therapy (PT) community. The purpose of this special interest article is to provide consensus-driven best practice resources developed by the Taskforce and describe the process of creating these resources to assist with telehealth implementation in neurologic PT practice, advocate for continued utilization, and shine light on opportunities for future research.

Summary of Key Points:
In this special interest article, we describe the process, challenges, and opportunities of developing and disseminating resources to educate, train, and support telehealth implementation in neurologic clinical practice. Four key strategies to facilitate telehealth implementation emerged: (1) increase knowledge of resources related to telehealth and mobile applications; (2) develop and disseminate evidence-based and consensus-based best practice recommendations for telehealth in neurologic PT; (3) provide future recommendations for integrating telehealth in PT, education, research, and clinical practice; and (4) encourage advocacy for inclusion of telehealth within the PT community. We explain the need to continue research and provide recommendations to expand telehealth research in neurologic clinical practice.

Recommendations for Clinical Practice:
This article highlights the potential and future of telehealth in neurologic PT practice. Our recommendations provide current clinical tools and resources for telehealth implementation following a knowledge-to-action framework and suggest areas for future research.

Video Abstract available for more insights from the authors (see the Video, the Supplemental Digital Content, available at: http://links.lww.com/JNPT/A447).
Orthosis use and ambulation in adults with myelomeningocele after orthotic management from childhood
Authors: Eriksson Marie , Bartonek Åsa 
Published: September 2023

Background:
Orthotic devices are required for walking in many individuals with myelomeningocele. Evidence concerning orthosis use is sparse, partly because of heterogeneity among groups and different definitions of the neurological level.

Objectives:
The objective of this study was to investigate ambulation regarding orthosis use and satisfaction with orthoses after intense orthotic management during childhood.

Study Design:
The study design is a retrospective follow-up with a cross-sectional study at adult age.

Methods:
Participants comprised 59 persons born in 1985 or later. Ambulation was categorized as community (Ca), household (Ha), nonfunctional (N-f), and nonambulation (N-a) groups. Orthosis use was registered at approximately 5 (Age5) and 12 (Age12) years of age and in adulthood (AdultAge). Satisfaction with orthoses was evaluated at AdultAge.

Results:
At Age5, Age12, and AdultAge, orthoses were used by 100%, 98%, and 78% of participants, respectively. Ambulation deteriorated between Age5 and Age12 in 17% of participants and between Age12 and AdultAge in 46%. At AdultAge, 63% maintained their ambulatory function; and muscle function and hip and knee flexion contractures were strongly correlated with ambulation. The ambulation groups did not differ regarding satisfaction with device or services, except in 2 single items where the Ha group differed in ratings from the N-f and N-a groups.

Conclusion:
The high frequency of orthosis use and similar satisfaction in all ambulation groups emphasize that early planning and follow-up of orthosis treatment during growth are important for mobility in adulthood. Our results also underline the importance of a close assessment of each individual's condition.
Using In Situ Simulation to Identify Latent Safety Threats in Emergency Medicine
Authors: Grace Margaret A. , O'Malley Roisin 
Published: September 2023

Summary Statement
This review aimed to explore existing literature on the use of in situ simulation to identify latent safety threats in emergency medicine. Studies were required to take place in a clinically active emergency department and have either a primary or secondary aim of identifying latent safety threats. A total of 2921 articles were retrieved through database searches and a total of 15 were deemed to meet the inclusion criteria.
Latent safety threats were detected by a variety of methods including documentation during debrief/discussion (66%), during the simulation itself (33%), participant surveys (20%), and video analysis (20%). Using a multimodality approach with input from observers and participants from different professional backgrounds yielded the highest number of threats per simulation case (43 per case). Equipment was the most commonly reported threat (83%), followed by teamwork/communication (67%). Some studies did not report on mitigation of identified risks; formal processes should be implemented for the management of latent safety threats identified by in situ simulation. Future research should focus on translational outcomes to further strengthen the position of in situ simulation in emergency medicine.
Impact of Umbilicus-Symphysis Pubis Distance on Technical Difficulties in Transabdominal Preperitoneal Hernia Repair (TAPP)
Authors: Arici Sinan 
Published: September 2023

Introduction:
Laparoscopic inguinal hernia repair has gained popularity due to its advantages over open procedures. However, technical challenges and anatomical variations can affect surgical outcomes. The umbilicus-symphysis pubis (USP) distance has been identified as a potential factor impacting laparoscopic operations. This study aims to investigate the association between USP distance, technical difficulties, and operative time in the transabdominal preperitoneal (TAPP) surgery.

Methods:
A retrospective analysis was conducted on 122 patients who underwent elective TAPP surgery. USP distance was measured, and surgical data were collected. Statistical analysis was performed to evaluate the relationship between USP distance and operative time.

Results:
Among the patients, 80 underwent unilateral hernia repair, and 42 underwent bilateral hernia repair. In unilateral repair, USP distance did not significantly affect operative time. However, in bilateral repair, there was a significant association between USP distance and operation time prolongation (P=0.039).

Discussion:
TAPP surgery presents challenges due to limited visualization and anatomical variations. Factors like USP distance can impact surgical outcomes. A shorter distance increases complexity, while a longer distance facilitates smoother procedures. Surgeons should consider USP distance during surgical planning to optimize outcomes.

Conclusion:
The study findings indicate that USP distance is associated with prolonged operative time in TAPP bilateral hernia repair. Surgeons, especially those in the learning phase, can benefit from considering this relationship to optimize surgical outcomes and resource allocation. Further research is needed to validate these findings and explore additional factors influencing operative time in hernia repair surgeries.
Comparative Risk of Type 2 Diabetes after Gastrectomy and Endoscopic Resection for Gastric Cancer: A Nationwide Cohort Study
Authors: Kwon Yeongkeun , Kwon Jin-Won , Kim Jiyun , Kim Dohyang , Hwang Jinseub , Ha Jane , Park Shin-Hoo , Park Sungsoo 
Published: September 2023

Background:
Patients with gastric cancer (GC) experience two characteristic treatment modalities (gastrectomy or endoscopic resection), which may induce heterogeneity in the risk of post-cancer treatment type 2 diabetes (T2D). We investigated differences in the risk of T2D development in GC survivors according to the two treatment methods.

Study design:
This retrospective nationwide population-based cohort study included 14,646 patients with GC who underwent gastrectomy (n=12,918) and endoscopic resection (n=1,728). We enrolled patients who survived for ≥5 years post gastrectomy or endoscopic resection, no history of diabetes, and had not received adjuvant chemotherapy. The T2D risk was evaluated using Cox regression for the gastrectomy group compared to that for the endoscopic resection group. Given the competing risks of incident T2D and death, a competing risk regression was performed.

Results:
After a median follow-up duration of 8.1 years, the incidence rates of T2D in the endoscopic resection group and gastrectomy group were 7.58 and 6.98 per 1000 person-years, respectively. Patients undergoing gastrectomy showed a significantly higher risk of developing T2D than patients undergoing endoscopic resection (hazard ratio [HR], 1.37; 95% confidence interval [CI], 1.18–1.58; P<0.0001). In subgroup analyses, gastrectomy was associated with increased T2D risk in female patients (HR, 1.72; 95% CI, 1.22–2.43; P=0.030 for interaction).

Conclusion:
Among GC survivors, patients undergoing gastrectomy showed a 37% increased risk of T2D development compared to patients undergoing endoscopic resection. Subgroup analyses showed that T2D risk increased by up to 72% in female patients. These results provide insights for establishing screening and preventive strategies for GC survivors to prevent T2D according to different treatment modalities.
Third dose COVID-19 mRNA vaccine enhances IgG4 isotype switching and recognition of Omicron subvariants by memory B cells after mRNA but not adenovirus priming
Authors: Hartley Gemma E. , Fryer Holly A. , Gill Paul A. , Boo Irene , Bornheimer Scott J. , Hogarth P. Mark , Drummer Heidi E. , O’Hehir Robyn E. , Edwards Emily S.J. , van Zelm Menno C. 
Published: September 2023

ABSTRACTBackgroundBooster vaccinations are recommended to improve protection against severe disease from SARS-CoV-2 infection. With primary vaccinations involving various adenoviral vector and mRNA-based formulations, it remains unclear if these differentially affect the immune response to booster doses. We here examined the effects of homologous (mRNA/mRNA) and heterologous (adenoviral vector/mRNA) vaccination on antibody and memory B cell (Bmem) responses against ancestral and Omicron subvariants.MethodsHealthy adults who received primary BNT162b2 (mRNA) (n=18) or ChAdOx1 (vector) (n=25) vaccination were sampled 1-month and 6-months after their 2nd and 3rd dose (homologous or heterologous) vaccination. Recombinant spike receptor-binding domain (RBD) proteins from ancestral, Omicron BA.2 and BA.5 variants were produced for ELISA-based serology, and tetramerized for immunophenotyping of RBD-specific Bmem.ResultsDose 3 boosters significantly increased ancestral RBD-specific plasma IgG and Bmem in both cohorts. Up to 80% of ancestral RBD-specific Bmem expressed IgG1+. IgG4+Bmem were detectable after primary mRNA vaccination, and expanded significantly to 5-20% after dose 3, whereas heterologous boosting did not elicit IgG4+Bmem. Recognition of Omicron BA.2 and BA.5 by ancestral RBD-specific plasma IgG increased from 20% to 60% after the 3rd dose in both cohorts. Reactivity of ancestral RBD-specific Bmem to Omicron BA.2 and BA.5 increased following a homologous booster from 40% to 60%, but not after a heterologous booster.ConclusionA 3rd mRNA dose generates similarly robust serological and Bmem responses in homologous and heterologous vaccination groups. The expansion of IgG4+Bmem after mRNA priming might result from the unique vaccine formulation or dosing schedule affecting the Bmem response duration and antibody maturation.
Poultry Ownership in Urban Kenya is Associated with Increased Fecal Contamination in Household Soil
Authors: Chan Elana M. G. , Swarthout Jenna M. , Chieng Benard , Araka Sylvie , Mboya John , Imali Christine , Harris Angela R. , Njenga Sammy M. , Pickering Amy J. 
Published: September 2023

AbstractAnimal feces can contain zoonotic enteropathogens capable of causing human diarrheal disease. Limited knowledge exists on domestic animal management in low-income urban settlements. We leveraged survey data and environmental samples collected from 120 urban Kenyan households to understand poultry husbandry practices and assess if household poultry ownership was associated withEscherichia colicontamination in stored water and soil. Fifty-five percent (n = 66) of households were in poultry-owning compounds, and 59.1% (n = 39) of these households reported poultry entering the household quarters. Among these 39 households, 53.9% (n = 21) kept poultry in the sleeping quarters of under-5 children. Household poultry ownership (49.2%, n = 59) was associated with increasedE. coliconcentrations in soil but not withE. coliprevalence in stored water. Poultry husbandry in urban settings may promote zoonotic disease transmission, and household soil may be an important transmission pathway for poultry-associated fecal contamination.
Generation and assessment of cytokine-induced killer cells for the treatment of colorectal cancer liver metastases
Authors: Ying Li Celine Man , Tomita Yoko , Dhakal Bimala , Tin Teresa , Li Runhao , Wright Josephine A , Vrbanac Laura , Woods Susan L , Drew Paul , Price Timothy , Smith Eric , Maddern Guy J , Fenix Kevin 
Published: September 2023

AbstractColorectal cancer (CRC) is the second leading cause of cancer-related death worldwide. Cytokine-induced killer (CIK) cells are an adoptive immunotherapy reported to have strong anti-tumour activity across a range of cancers. They are a heterogeneous mix of lymphoid cells generated by culturing human peripheral blood mononuclear cells (PBMCs) with cytokines and monoclonal antibodiesin vitro. We compared CIK cells from healthy donors and donors with CRC liver metastases, generated in RPMI supplemented with 10% fetal bovine serum (FBS) or three different serum-free media. CIK cells generated in serum free medium X-VIVO 15 were comparable to those from RPMI medium with 10% FBS in terms of the number and percentages of the main subsets of cells in the CIK culture, and the intracellular levels of granzyme B and perforin, and the pro-inflammatory cytokines IL-2, IFN-γ and TNF-α. There was no significant difference in cytotoxicity against CRC cell lines grown in 2D cultures or as spheroids, and against autologous patient-derived tumour organoids. CIK cells from patients with CRC liver metastases grown in X-VIVO 15 media were similar to those from healthy donors in each of the measures. Donor attributes such as age, sex, or prior chemotherapy exposure had no significant impact on CIK cell numbers or function. These results support further investigations into the therapeutic application of autologous CIK cells in the management of patients with CRC liver metastases.
Preclinical exploration of the DNA Damage Response pathway using the interactive neuroblastoma cell line explorer CLEAN
Authors: Gabre Jonatan L. , Merseburger Peter , Claeys Arne , Siaw Joachim , Bekaert Sarah-Lee , Speleman Frank , Hallberg Bengt , Palmer Ruth H. , Van den Eynden Jimmy 
Published: September 2023

ABSTRACTNeuroblastoma (NB) is the most common cancer in infancy with an urgent need for more efficient targeted therapies. The development of novel (combinatorial) treatment strategies relies on extensive explorations of signaling perturbations in neuroblastoma cell lines, using RNA-Seq or other high throughput technologies (e.g., phosphoproteomics). This typically requires dedicated bioinformatics support, which is not always available. Additionally, while data from published studies are highly valuable and raw data (e.g., fastq files) are nowadays released in public repositories, data processing is time-consuming and again difficult without bioinformatics support. To facilitate NB research, more user-friendly and immediately accessible platforms are needed to explore newly generated as well as existing high throughput data. To make this possible, we developed an interactive data centralization and visualization web application, called CLEAN (the Cell Line Explorer web Application of Neuroblastoma data;https://ccgg.ugent.be/shiny/clean/). By focusing on the regulation of the DNA damage response, a therapeutic target of major interest in neuroblastoma, we demonstrate how CLEAN can be used to gain novel mechanistic insights and identify putative drug targets in neuroblastoma.
A Parasite Odyssey: An RNA virus concealed inToxoplasma gondii
Authors: Gupta Purav , Hiller Aiden , Chowdhury Jawad , Lim Declan , Lim Dillon Yee , Saeij Jeroen P.J. , Babaian Artem , Rodriguez Felipe , Pereira Luke , Morales Alex 
Published: September 2023

AbstractWe are entering a “Platinum Age of Virus Discovery”, an era marked by exponential growth in the discovery of virus biodiversity, and driven by advances in metagenomics and computational analysis. In the ecosystem of a human (or any animal) there are more species of viruses than simply those directly infecting the animal cells. Viruses can infect all organisms constituting the microbiome, including bacteria, fungi, and unicellular parasites. Thus the complexity of possible interactions between host, microbe, and viruses is unfathomable. To understand this interaction network we must employ computationally-assisted virology as a means of analyzing and interpreting the millions of available samples to make inferences about the ways in which viruses may intersect human health.From a computational viral screen of human neuronal datasets, we identified a novel narnavirusApocryptovirus odysseus(Ao) which likely infects the neurotropic parasiteToxoplasma gondii. Previously, several parasitic protozoan viruses (PPVs) have been mechanistically established as triggers of host innate responses, and here we presentin silicoevidence that Ao is a plausible pro-inflammatory factor in human and mouse cells infected byT. gondii.T. gondiiinfects billions of people worldwide, yet the prognosis of toxoplasmosis disease is highly variable, and PPVs like Ao could function as a hitherto undescribed hypervirulence factor. In a broader screen of over 7.6 million samples, we explored phylogenetically-proximal viruses to Ao and discovered 19Apocryptovirusspecies, all found in libraries annotated as vertebrate transcriptome or metatranscriptomes. While the Narnavirus samples making up this genus-like clade are derived from sheep, goat, bat, rabbit, chicken, and pigeon samples, the presence of virus is strongly predictive of parasitic (Apicomplexa) nucleic acid co-occurrence, supporting that these are a clade of parasite-infecting viruses.This is a computational proof-of-concept study in which we rapidly analyze millions of datasets from which we distilled a mechanistically, ecologically, and phylogenetically refined hypothesis. We predict this highly diverged Ao RNA virus is biologically aT. gondiiinfection, and that Ao, and other viruses like it, will modulate this disease which afflicts billions worldwide.
A meta-analysis of bulk RNA-seq datasets identifies potential biomarkers and repurposable therapeutics against Alzheimer’s disease
Authors: Lamisa Anika Bushra , Ahammad Ishtiaque , Bhattacharjee Arittra , Hossain Mohammad Uzzal , Ishtiaque Ahmed , Chowdhury Zeshan Mahmud , Das Keshob Chandra , Salimullah Md , Keya Chaman Ara 
Published: September 2023

AbstractAlzheimer’s disease (AD) poses a major challenge due to its impact on the elderly population and the lack of effective early diagnosis and treatment options. In an effort to address this issue, a study focused on identifying potential biomarkers and therapeutic agents for AD was carried out. Using RNA-Seq data from AD patients and healthy individuals, 12 differentially expressed genes (DEGs) were identified, with 9 expressing upregulation (ISG15, HRNR, MTATP8P1, MTCO3P12, DTHD1, DCX, ST8SIA2, NNAT,andPCDH11Y) and 3 expressing downregulation (LTF, XIST,andTTR). Among them,TTRexhibited the lowest gene expression profile. Interestingly, functional analysis tiedTTRto amyloid fiber formation and neutrophil degranulation through enrichment analysis. These findings suggested the potential ofTTRas a diagnostic biomarker for AD. Additionally, druggability analysis revealed that the FDA-approved drug Levothyroxine might be effective against the Transthyretin protein encoded by theTTRgene. Molecular docking and dynamics simulation studies of Levothyroxine and Transthyretin suggested that this drug could be repurposed to treat AD. However, additional studies using in vitro and in vivo models are necessary before these findings can be applied in clinical applications.