bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis identification of A-to-I RNA editing in granulosa cells associated with PCOS
Authors: Kong Fan-Sheng , Lu Zijing , Zhou Yuan , Lu Yinghua , Ren Chun-Yan , Jia Ruofan , Zeng Beilei , Huang Panwang , Wang Jihong , Ma Yaping , Chen Jian-Huan 
Published: July 2023

BackgroundPolycystic ovary syndrome (PCOS) is a complex, multifactor disorder in women of reproductive age worldwide. Although RNA editing may contribute to a variety of diseases, its role in PCOS remains unclear.MethodsA discovery RNA-Seq dataset was obtained from the NCBI Gene Expression Omnibus database of granulosa cells from women with PCOS and women without PCOS (controls). A validation RNA-Seq dataset downloaded from the European Nucleotide Archive Databank was used to validate differential editing. Transcriptome-wide investigation was conducted to analyze adenosine-to-inosine (A-to-I) RNA editing in PCOS and control samples.ResultsA total of 17,395 high-confidence A-to-I RNA editing sites were identified in 3,644 genes in all GC samples. As for differential RNA editing, there were 545 differential RNA editing (DRE) sites in 259 genes with Nucleoporin 43 (NUP43), Retinoblastoma Binding Protein 4 (RBBP4), and leckstrin homology-like domain family A member 1 (PHLDA) showing the most significant three 3′-untranslated region (3′UTR) editing. Furthermore, we identified 20 DRE sites that demonstrated a significant correlation between editing levels and gene expression levels. Notably, MIR193b-365a Host Gene (MIR193BHG) and Hook Microtubule Tethering Protein 3 (HOOK3) exhibited significant differential expression between PCOS and controls. Functional enrichment analysis showed that these 259 differentially edited genes were mainly related to apoptosis and necroptosis pathways. RNA binding protein (RBP) analysis revealed that RNA Binding Motif Protein 45 (RBM45) was predicted as the most frequent RBP binding with RNA editing sites. Additionally, we observed a correlation between editing levels of differential editing sites and the expression level of the RNA editing enzyme Adenosine Deaminase RNA Specific B1 (ADARB1). Moreover, the existence of 55 common differentially edited genes and nine differential editing sites were confirmed in the validation dataset.ConclusionOur current study highlighted the potential role of RNA editing in the pathophysiology of PCOS as an epigenetic process. These findings could provide valuable insights into the development of more targeted and effective treatment options for PCOS.
Effect of vaginal microbiota on pregnancy outcomes of women from Northern China who conceived after IVF
Authors: Tong Yu , Sun Qiang , Shao Xiaoguang , Wang Zhijian 
Published: July 2023

ObjectiveThis study aimed to investigate the correlation between vaginal microbiota and pregnancy outcomes of women who achieved pregnancy via in vitro fertilization (IVF) in Northern China, and to determine a biomarker for evaluation of the risk of preterm births in these women.MethodsIn total, 19 women from Northern China women who conceived after IVF and 6 women who conceived naturally were recruited in this study. The vaginal samples of the healthy participants were collected throughout pregnancy, that is, during the first, second, and third trimesters. The V3–V4 region of 16S rRNA was used to analyze the vaginal microbiome, and the bioinformatic analysis was performed using QIIME Alpha and Beta diversity analysis.ResultsEither IVF group or Natural conception group, bacterial community diversities and total species number of vagnal samples from who delivered at term were significantly higher than those who delivered before term. Low abundance of vaginal bacteria indicates an increased risk of preterm delivery. Further, more abundant vaginal bacteria was found in first trimesters instead of the next two trimesters. Vignal samples collected during first trimester showed richer differences and more predictive value for pregnancy outcoes. In addition, the diversity of the vaginal bacterial community decreased as the gestational age increased, in all samples. Alloscardovia was only found in participants who conceived after IVF, and the percentage of Alloscardovia in viginal samples of normal delivery group is much higher than the samples from preterm delivery group.Vobrio specifically colonized in vagina of pregnant woman in AFT group (those who conceived after IVF (A), first trimester (F), and delivered at term (T)) and Sporosarcina was detected only in women with AFT and AST (those who conceived after IVF (A), second trimester (S), and delivered at term (T)). These data indicates that Alloscardovia, Vobrio and Sporosarcina have great potential in predicting pregnancy outcomes who pregnanted by vitro fertilizationConclusionsVaginal microbiota were more stable in women who conceived naturally and those who carried pregnancy to term. Oceanobacillus might act as a positive biomarker, whereas Sulfurospirillum and Propionispira may act as negative biomarkers for the risk of preterm birth.
Low T-cell reactivity to TDP-43 peptides in ALS
Authors: Ramachandran Swetha , Grozdanov Veselin , Leins Bianca , Kandler Katharina , Witzel Simon , Mulaw Medhanie , Ludolph Albert C. , Weishaupt Jochen H. , Danzer Karin M. 
Published: July 2023

BackgroundDysregulation of the immune system in amyotrophic lateral sclerosis (ALS) includes changes in T-cells composition and infiltration of T cells in the brain and spinal cord. Recent studies have shown that cytotoxic T cells can directly induce motor neuron death in a mouse model of ALS and that T cells from ALS patients are cytotoxic to iPSC-derived motor neurons from ALS patients. Furthermore, a clonal expansion to unknown epitope(s) was recently found in familial ALS and increased peripheral and intrathecal activation of cytotoxic CD8+ T cells in sporadic ALS.ResultsHere, we show an increased activation of peripheral T cells from patients with sporadic ALS by IL-2 treatment, suggesting an increase of antigen-experienced T cells in ALS blood. However, a putative antigen for T-cell activation in ALS has not yet been identified. Therefore, we investigated if peptides derived from TDP-43, a key protein in ALS pathogenesis, can act as epitopes for antigen-mediated activation of human T cells by ELISPOT and flow cytometry. We found that TDP-43 peptides induced only a weak MHCI or MHCII-restricted activation of both naïve and antigen-experienced T cells from healthy controls and ALS patients. Interestingly, we found less activation in T cells from ALS patients to TDP-43 and control stimuli. Furthermore, we found no change in the levels of naturally occurring auto-antibodies against full-length TDP-43 in ALS.ConclusionOur data suggests a general increase in antigen-experienced T cells in ALS blood, measured by in-vitro culture with IL-2 for 14 days. Furthermore, it suggests that TDP-43 is a weak autoantigen.
Intratumoral NKp46+ natural killer cells are spatially distanced from T and MHC-I+ cells with prognostic implications in soft tissue sarcoma
Authors: Cruz Sylvia M. , Sholevar Cyrus J. , Judge Sean J. , Darrow Morgan A. , Iranpur Khurshid R. , Farley Lauren E. , Lammers Marshall , Razmara Aryana M. , Dunai Cordelia , Gingrich Alicia A. , Persky Julia , Mori Hidetoshi , Thorpe Steven W. , Monjazeb Arta M. , Murphy William J. , Canter Robert J. 
Published: July 2023

IntroductionSoft tissue sarcomas (STS) are rare, heterogenous malignancies with an unmet need for novel immunotherapies. Tumor infiltrating lymphocytes (TILs) have been linked with favorable outcomes in STS patients, though the contribution of natural killer (NK) cells and spatial relationships of TILs with MHC-I expressing cells lacks detailed characterization.Experimental designUsing archived and prospectively collected specimens, we evaluated intratumoral NK cells by immunohistochemistry (IHC), flow cytometry, and immunofluorescence (IF). We assessed spatial localization of NK and T cells by multiplex IF, analyzing the effects of MHC-I expression status on NK and T cell clustering.ResultsBoth intratumoral NKp46 and CD56dim expression were associated with significantly improved overall survival (P=0.05), while higher infiltrates of CD56bright NK cells predicted a worse prognosis (P=0.05). The presence of intratumoral NK cells was inversely proportional to CD3+ T cells. Spatial analyses showed NK cells preferentially clustering close to other NK cells with sparse CD3+ T and CD8+ T cells in range (P&lt;0.0001). Additionally, CD3+ T and CD8+ T cells showed significantly greater co-localization with MHC-I+ cells, compared to NK cells (P&lt;0.0001). After neoadjuvant radiotherapy, there was greater CD8 clustering, while after neoadjuvant chemotherapy, there was overall lower TIL clustering.ConclusionIntratumoral NK cells are prognostic in STS and localize closer to MHC-I- cells than T cells. Although both NK and T cells are associated with improved survival in STS, their differential distribution in the TME based on MHC-I expression status may serve as a biomarker for improved immunotherapy treatment selection.
Association between enlarged perivascular spaces and cerebrospinal fluid aquaporin-4 and tau levels: report from a memory clinic
Authors: Sacchi Luca , Arcaro Marina , Carandini Tiziana , Pietroboni Anna Margherita , Fumagalli Giorgio Giulio , Fenoglio Chiara , Serpente Maria , Sorrentino Federica , Visconte Caterina , Pintus Manuela , Conte Giorgio , Contarino Valeria Elisa , Scarpini Elio , Triulzi Fabio , Galimberti Daniela , Arighi Andrea 
Published: July 2023

BackgroundPerivascular spaces (PVS) are fluid-filled compartments that dilate in response to many different conditions. A high burden of enlarged PVS (EPVS) in the centrum semiovale (CSO) has been linked to neurodegeneration. Moreover, an increase in cerebrospinal fluid (CSF) levels of aquaporin-4 (AQP4), a water channel expressed on PVS-bounding astrocytes, has been described in patients with neurodegenerative dementia. Our aim was to investigate the relationship between neurodegenerative diseases and two putative glymphatic system biomarkers: AQP4 and EPVS.MethodsWe included 70 individuals, 54 patients with neurodegenerative diseases and 16 subjects with non-degenerative conditions. EPVS were visually quantified on MRI-scans applying Paradise’s scale. All subjects underwent lumbar puncture for the measurement of AQP4 levels in the cerebrospinal fluid (CSF). CSF levels of amyloid-β-1-42, phosphorylated and total tau (tTau) were also measured. Linear regression analyses were adjusted for age, sex, education and disease duration, after excluding outliers.ResultsCerebrospinal fluid (CSF)-AQP4 levels were independent predictors of total (β = 0.28, standard error [SE] = 0.08, p = 0.001), basal ganglia (β = 0.20, SE = 0.08, p = 0.009) and centrum semiovale EPVS (β = 0.37, SE = 0.12, p = 0.003). tTau levels predicted CSO-EPVS (β = 0.30, SE = 0.15, p = 0.046). Moreover, increased levels of AQP4 were strongly associated with higher levels of tTau in the CSF (β = 0.35, SE = 0.13, p = 0.008).ConclusionWe provide evidence that CSO-EPVS and CSF-AQP4 might be clinically meaningful biomarkers of glymphatic dysfunction and associated neurodegeneration.
The interaction between the major vault protein rs4788186 polymorphism, alcohol dependence, and depression among male Chinese problem drinkers
Authors: Wu Yuyu , Zhao Ke , Chen Yingjie , Wu Liujun , Qiu Feng , Yuan Yuying , Shen Guanghui , Wang Kexin , Kang Yimin , Jiang Yongsheng , Wang Wei , Chen Li , Liu Yanlong , Pan Xuebo , Wang Fan , Xie Longteng 
Published: July 2023

ObjectiveAlcohol use disorder (AUD) is the second most prevalent mental disorder and might be related to depression. Major vault protein (MVP) is a cytoplasmic protein related to vesicle transport. The present study aimed to investigate the interaction between a genetic variant (MVP rs4788186) and depression in adult male Han Chinese with AUD during withdrawal.MethodsAll participants (N = 435) were diagnosed with AUD. Alcohol dependence level was measured using the Michigan Alcoholism Screening Test, and depression was measured using the self-rating depression scale. Genomic DNA was extracted from peripheral blood and genotyped.ResultsHierarchical regression analysis identified an interaction between MVP rs4788186 and alcohol dependence level for depression (β = −0.17, p &lt; 0.05). Then, a region of significance test was performed to interpret the interaction effect. Re-parameterized regression models revealed that the interaction between MVP rs4788186 and alcohol problem severity fit the strong differential susceptibility model (R2 = 0.08, p &lt; 0.001), suggesting that the AA homozygotes would be more likely subjects with the G allele to experience major depression symptoms.ConclusionCarriers of the AA homozygote of MVP rs4788186 may be more susceptible to severe alcohol problems and higher levels of depression during withdrawal.
The voice characterisation checklist: psychometric properties of a brief clinical assessment of voices as social agents
Authors: Edwards Clementine J. , Owrid Oliver , Miller Lucy , Jafari Hassan , Emsley Richard , Rus-Calafell Mar , Craig Thomas K. J. , Clancy Moya , McLeod Hamish , Fornells-Ambrojo Miriam , McDonnell Jeffrey , Montague Alice , Huckvale Mark , Bucci Sandra , Haddock Gillian , Garety Philippa , Ward Thomas 
Published: July 2023

AimThere is growing interest in tailoring psychological interventions for distressing voices and a need for reliable tools to assess phenomenological features which might influence treatment response. This study examines the reliability and internal consistency of the Voice Characterisation Checklist (VoCC), a novel 10-item tool which assesses degree of voice characterisation, identified as relevant to a new wave of relational approaches.MethodsThe sample comprised participants experiencing distressing voices, recruited at baseline on the AVATAR2 trial between January 2021 and July 2022 (n = 170). Inter-rater reliability (IRR) and internal consistency analyses (Cronbach’s alpha) were conducted.ResultsThe majority of participants reported some degree of voice personification (94%) with high endorsement of voices as distinct auditory experiences (87%) with basic attributes of gender and age (82%). While most identified a voice intention (75%) and personality (76%), attribution of mental states (35%) to the voice (‘What are they thinking?’) and a known historical relationship (36%) were less common. The internal consistency of the VoCC was acceptable (10 items, α = 0.71). IRR analysis indicated acceptable to excellent reliability at the item-level for 9/10 items and moderate agreement between raters’ global (binary) classification of more vs. less highly characterised voices, κ = 0.549 (95% CI, 0.240–0.859), p &lt; 0.05.ConclusionThe VoCC is a reliable and internally consistent tool for assessing voice characterisation and will be used to test whether voice characterisation moderates treatment outcome to AVATAR therapy. There is potential wider utility within clinical trials of other relational therapies as well as routine clinical practice.
MAPA DE HERIDAS IMPUNES
Authors: Fernandez Villanueva Maria Concepcion , Ortega Abarca Paulina Belén 
Published: July 2023

El trabajo pone de manifiesto las deficiencias en los procedimientos jurídicos actuales para apreciar adecuadamente los delitos de violencia sexual contra menores lo que puede conducir a conclusiones absolutorias e impunidad. Partimos del análisis cualitativo de contenido y de discurso de 44 sentencias absolutorias de violencia sexual sobre menores emitidas por los tribunales españoles en el período 1/5/2020 a 31/12/2020. los resultados constatan varios procesos y circunstancias que causan lo que hemos llamado heridas: 1.-un escenario prevalente de abusos y violencia en situaciones de desprotección de los menores, ya sean el ámbito familiar o institucional lo que dificulta y retrasa la formulación de las denuncias. 2.- la dificultad de conseguir y apreciar las pruebas de los daños físicos y psicológicos, condicionados por la situación de desigualdad. 3.-la falta de credibilidad de las víctimas y la distorsión en la apreciación de los motivos de la violencia de los agresores.
Palabras clave:
Violencia sexual, menores, absoluciones, deficiencias jurídicas, España
Trends in heat and cold wave risks for the Italian Trentino-Alto Adige region from 1980 to 2018
Authors: Morlot Martin , Russo Simone , Feyen Luc , Formetta Giuseppe 
Published: July 2023

Abstract. Heat waves (HWs) and cold waves (CWs) can have considerable impact on
people. Mapping risks of extreme temperature at local scale, accounting for
the interactions between hazard, exposure, and vulnerability, remains a
challenging task. In this study, we quantify risks from HWs and CWs for the
Trentino-Alto Adige region of Italy from 1980 to 2018 at high spatial
resolution. We use the Heat Wave Magnitude Index daily (HWMId) and the Cold
Wave Magnitude Index daily (CWMId) as the hazard indicators. To obtain HWs
and CW risk maps we combined the following: (i) occurrence probability maps of the hazard
obtained using the zero-inflated Tweedie distribution (accounting directly
for the absence of events for certain years), (ii) normalized population
density maps, and (iii) normalized vulnerability maps based on eight
socioeconomic indicators. The methodology allowed us to disentangle the
contributions of each component of the risk relative to total change in
risk. We find a statistically significant increase in HW hazard and
exposure, while CW hazard remained stagnant in the analyzed area over the
study period. A decrease in vulnerability to extreme temperature spells is
observed through the region except in the larger cities where vulnerability
increased. HW risk increased in 40 % of the region, with the increase
being greatest in highly populated areas. Stagnant CW hazard and declining
vulnerability result in reduced CW risk levels overall, except for the four
main cities where increased vulnerability and exposure increased risk
levels. These findings can help to steer investments in local risk
mitigation, and this method can potentially be applied to other regions
where there are sufficient detailed data.
Khái Niệm Công Bình Xã Hội Theo Sách Amos
Authors: Lê Văn Luận 
Published: July 2023

Từ thời kỳ đầu của các ngôn sứ, việc đấu tranh cho công bình cũng đã xuất hiện, cách rõ nét nhất nơi sách ngôn sứ Amos. Bài viết này khảo sát và tìm về với khái niệm công bình xã hội trong sách Amos – nền công bình có nền tảng từ Thiên Chúa, và hướng đến việc thăng tiến và đảm bảo nhân phẩm con người. Từ đó, bài viết đưa ra những điểm phản tỉnh hầu có thể soi sáng phần nào cho thao thức thăng tiến công bình xã hội, một sứ mạng luôn gắn liền với việc bảo vệ đức tin.

Abstract
Since the early days of the prophets, the struggle for justice has been evident, most notably in the Book of Amos. This article explores and delves into the concept of social justice in the Book of Amos – a justice rooted in God and aimed at promoting and ensuring human dignity. From there, the article presents insightful points that can shed some light on the conscientious pursuit of social justice, a mission intrinsically linked to the protection of faith.
ДОСЛІДЖЕННЯ КОЛЕКЦІЇ МЛИНАРСЬКИХ СПОРУД НАЦІОНАЛЬНОГО ІСТОРИКО-ЕТНОГРАФІЧНОГО ЗАПОВІДНИКА «ПЕРЕЯСЛАВ» (1954–2022 рр.): історіографія
Authors: Vovkodav Viktoriia 
Published: July 2023

У статті здійснено історіографічний огляд праць, присвячених пам’яткам науки й техніки місцевого значення – вітрякам та водяним млинам НІЕЗ «Переяслав». Метою дослідження є аналіз та систематизація публікацій, у яких розглянуто цю проблематику, написаних упродовж 1954–2022 рр. Методологічну основу дослідження становлять загальнонаукові та історичні методи, принципи наукового об’єктивізму, комплексності, системності та історизму. Використання таких теоретико-методологічних конструкцій дало можливість сформувати дослідницьку логіку та цілісну картину процесу наукового вивчення колекції вітряних та водяних млинів Музею народної архітектури та побуту Середньої Наддніпрянщини протягом цього періоду, а також показати основні тенденції осмислення наукового доробку дослідників і представити комплексний аналіз літератури. Наукова новизна. Ця студія є першою спробою визначення рівня дослідження збірки млинів Заповідника на базі наукової літератури зазначеного періоду. Висновки. Аналіз стану вивчення порушеної проблематики дав можливість з’ясувати, що наведені публікації є не лише важливою історіографічною одиницею дослідження, а й представляють значну за обсягом базу для вивчення історії формування колекції вітряків та водяників НІЕЗ «Переяслав». Водночас наявність великої кількості спеціалізованих праць, у яких розглянуті питання, пов’язані з названими спорудами, свідчить про неабиякий науковий інтерес до них як до об’єктів окремих студій. А існування великої кількості публікацій різного тематичного спрямування, де в різному контексті згадуються млини Переяславського скансену, доповнює загальний науковий простір їх дослідження.
Learning in a crisis moment: a randomized controlled trial in emergency bystander intervention
Authors: McGaffick Caitlin , Gulrajani Noor , Kong Nan , Adams Nicole 
Published: July 2023

Abstract
Background
Opioid overdose is the leading cause of injury-related death in the United States. Individuals who overdose outside of clinical settings have more positive clinical outcomes when they receive naloxone, an opioid antagonist, from a bystander as an early intervention before emergency personnel arrive. However, there is a gap in knowledge about successful instantaneous learning and intervention in a real-life stressful environment. The objective of this study is to explore the efficacy of different instructional delivery methods for bystanders in a stressful environment. We aim to evaluate which methods are most effective for instantaneous learning, successful intervention, and improved clinical outcomes.

Methods
To explore instantaneous learning in a stressful environment, we conducted a quantitative randomized controlled trial to measure how accurately individuals responded to memory-based survey questions guided by different instructional methods. Students from a large university in the Midwest (n = 157) were recruited in a public space on campus and accessed the six-question survey on their mobile devices. The intervention group competed the survey immediately while the research team created a distracting environment. The control group was asked to complete the survey later in a quiet environment.

Results
The intervention group correctly answered 0.72 questions fewer than the control group (p = .000, CI [0.337, 1.103]). Questions Q1 and Q5 contained direct instructions with a verbal component and showed the greatest accuracy with over 90% correct for both stressful and controlled environments.

Conclusions
The variability in the responses suggests that there are environmental factors as well as instructional design features which influence instantaneous learning. The findings of this study begin to address the gap in knowledge about the effects of stress on instantaneous learning and the most effective types of instruction for untrained bystanders in emergency situations.