bluuebunny/crossref_metadata_2025_split huggingface dataset:
Focus - Costruire la genitorialità in condizioni di incertezza
Authors: Fargion Silvia , Sanfelici Mara , Gui Luigi , Nothdurfter Urban , Nagy Andrea , Monaco Salvatore , Falcone Francesca , Samà Antonio , Mauri Diletta 
Published: January 2023

- Un progetto per la comprensione della genitorialità in contesti di particolare incertezza- Genitori intrappolati e genitori possibili tra povertà economica e servizi del welfare- Genitorialità LGBT+ nell'Italia di oggi: immaginarsi, diventare e fare i genitori tra incertezze e risorse- "Navigare" le incertezze. Le esperienze di genitori in migrazione forzata- Riposizionarsi come genitori in un tempo frammentato e conteso
Faktor Kejadian Stunting Pada Balita : Systematic Review
Authors: Khoiriyah Hikmatul , Ismarwati Ismarwati 
Published: January 2023

Abstrak
Masalah kesehatan yang terjadi pada balita salah satunya adalah kejadian stunting. Stunting merupakan kondisi balita mengalami kekurangan asupan nutrisi dalam jangka waktu yang cukup lama sehingga anak mengalami gangguan pertumbuhan yaitu tinggi badan lebih pendek dari standar usia. Penyebab kejadian stunting adalah ketidakseimbangan asupan nutrisi dalam 1000 hari pertama kehidupan. Systematic review ini bertujuan untuk menemukan bukti berdasarkan artikel yang telah dipublikasikan terkait faktor kejadian stunting pada balita. Kriteria yang termasuk pada review ini adalah artikel original, artikel yang diterbitkan dalam Bahasa Inggris, artikel yang membahas tentang faktor kejadian stunting pada balita dan jurnal terbitan tahun 2017-2022. Metode review menggunakan systematic review dengan menggunakan PRISMA For Systematic Review,yang didapatkan dari database Ebsco, Pubmed, Scient direct, Proquest. Hasil review didapatkan 15 dari 1006 artikel yang dipilih. Kejadian stunting pada balita dikelompokkan dalam 4 tema yaitu faktor anak, faktor ibu, faktor lingkungan dan pola makan. Kesimpulan pada review ini didapatkan artikel terkait faktor penyebab stunting sehingga disarankan peneliti selanjutnya melakukan penelitian terkait penanganan stunting berdasarkan faktor penyebabnya. Hal tersebut bertujuan agar masalah stunting pada balita dapat terselesaikan.
Proyecto interdisciplinario frente a las diferencias sociales, culturales e individuales de los estudiantes
Authors: Guaita Lagua Luz Adelaida , Guaita Lagua Zoila Clemencia , Guaita Lagua Segundo Fidel , Guaita Lagua Segundo Martín 
Published: January 2023

Introducción: la implementación de proyectos interdisciplinarios es una estrategia adoptada por el Ministerio de Educación del Ecuador para resaltar el desarrollo de habilidades cognitivas, socioemocionales y procedimentales de los y las estudiantes en el marco del trabajo cooperativo y la metodología de aprendizaje basado en proyectos. Además, las relaciones interdisciplinarias constituyen una vía que posibilita perfeccionar el proceso de enseñanza aprendizaje y la formación de profesionales de una manera integral y holística. Objetivos: este trabajo se desarrolla con la finalidad de determinar cómo la aplicación de proyectos interdisciplinarios contribuye en la enseñanza para la diversidad, tomando en cuenta la heterogeneidad del alumnado, en especial de los estudiantes provenientes de diversos pueblos y nacionalidades en el marco de una educación intercultural. Metodología: esta investigación se realizó mediante un enfoque cualitativo y cuantitativo que permitió apreciar la información obtenida sobre la práctica educativa incluyente en instituciones de pueblos originarios. Para esto se apoyó en la modalidad bibliográfica y de Campo con la sustentación teórica y acercamiento a la realidad educativa. El trabajo investigativo presenta un nivel exploratorio y descriptivo. La técnica que se aplicó fue la encuesta con una guía de 10 preguntas dirigidas a 20 docentes que laboran en diferentes Instituciones Educativas. Resultados: los resultados expresan que trabajar con proyectos interdisciplinarios promueven la creatividad, fomenta el trabajo colaborativo y motiva al trabajo autónomo. Conclusiones: sin embargo, los resultados resaltan que la atención a la diversidad es limitada, debido a que no se toma en consideración los saberes y conocimientos propios de los estudiantes provenientes de diferentes culturas, con la finalidad de convivir armónicamente entre mestizos, indígenas, afrodescendientes, montubios y de otras culturas. Área de estudio o rama de la ciencia: Educación Básica.
Functionally active TRPA1 ion channel is downregulated in peptidergic neurons of the Edinger-Westphal nucleus upon acute alcohol exposure
Authors: Al-Omari Ammar , Kecskés Miklós , Gaszner Balázs , Biró-Sütő Tünde , Fazekas Balázs , Berta Gergely , Kuzma Mónika , Pintér Erika , Kormos Viktória 
Published: January 2023

Introduction:The centrally projecting Edinger-Westphal nucleus (EWcp) contributes to the control of alcohol consumption by its urocortin 1 (UCN1) and cocaine- and amphetamine-regulated transcript (CART) co-expressing peptidergic neurons. Our group recently showed that the urocortinergic centrally projecting EWcp is the primary seat of central nervous system transient receptor potential ankyrin 1 (TRPA1) cation channel mRNA expression. Here, we hypothesized that alcohol and its metabolites, that pass through the blood-brain barrier, may influence the function of urocortinergic cells in centrally projecting EWcp by activating TRPA1 ion channels. We aimed to examine the functional activity of TRPA1 in centrally projecting EWcp and its possible role in a mouse model of acute alcohol exposure.Methods:Electrophysiological measurements were performed on acute brain slices of C57BL/6J male mice containing the centrally projecting EWcp to prove the functional activity of TRPA1 using a selective, potent, covalent agonist JT010. Male TRPA1 knockout (KO) and wildtype (WT) mice were compared with each other in the morphological studies upon acute alcohol treatment. In both genotypes, half of the animals was treated intraperitoneally with 1 g/kg 6% ethanol vs. physiological saline-injected controls. Transcardial perfusion was performed 2 h after the treatment. In the centrally projecting EWcp area, FOS immunohistochemistry was performed to assess neuronal activation. TRPA1, CART, and urocortin 1 mRNA expression as well as urocortin 1 and CART peptide content was semi-quantified by RNAscopein situhybridization combined with immunofluorescence.Results:JT010 activated TRPA1 channels of the urocortinergic cells in acute brain slices. Alcohol treatment resulted in a significant FOS activation in both genotypes. Alcohol decreased theTrpa1mRNA expression in WT mice. The assessment of urocortin 1 peptide immunoreactivity revealed lower basal urocortin 1 in KO mice compared to WTs. The urocortin 1 peptide content was affected genotype-dependently by alcohol: the peptide content decreased in WTs while it increased in KO mice. Alcohol exposure influenced neither CART and urocortin 1 mRNA expression nor the centrally projecting EWcp/CART peptide content.Conclusion:We proved the presence of functional TRPA1 receptors on urocortin 1 neurons of the centrally projecting EWcp. DecreasedTrpa1mRNA expression upon acute alcohol treatment, associated with reduced neuronal urocortin 1 peptide content suggesting that this cation channel may contribute to the regulation of the urocortin 1 release.
The tendon interfascicular basement membrane provides a vascular niche for CD146+ cell subpopulations
Authors: Marr Neil , Zamboulis Danae E. , Werling Dirk , Felder Alessandro A. , Dudhia Jayesh , Pitsillides Andrew A. , Thorpe Chavaunne T. 
Published: January 2023

Introduction: The interfascicular matrix (IFM; also known as the endotenon) is critical to the mechanical adaptations and response to load in energy-storing tendons, such as the human Achilles and equine superficial digital flexor tendon (SDFT). We hypothesized that the IFM is a tendon progenitor cell niche housing an exclusive cell subpopulation.Methods: Immunolabelling of equine superficial digital flexor tendon was used to identify the interfascicular matrix niche, localising expression patterns of CD31 (endothelial cells), Desmin (smooth muscle cells and pericytes), CD146 (interfascicular matrix cells) and LAMA4 (interfascicular matrix basement membrane marker). Magnetic-activated cell sorting was employed to isolate and compare in vitro properties of CD146+ and CD146− subpopulations.Results: Labelling for CD146 using standard histological and 3D imaging of large intact 3D segments revealed an exclusive interfascicular cell subpopulation that resides in proximity to a basal lamina which forms extensive, interconnected vascular networks. Isolated CD146+ cells exhibited limited mineralisation (osteogenesis) and lipid production (adipogenesis).Discussion: This study demonstrates that the interfascicular matrix is a unique tendon cell niche, containing a vascular-rich network of basement membrane, CD31+ endothelial cells, Desmin+ mural cells, and CD146+ cell populations that are likely essential to tendon structure and/or function. Contrary to our hypothesis, interfascicular CD146+ subpopulations did not exhibit stem cell-like phenotypes. Instead, our results indicate CD146 as a pan-vascular marker within the tendon interfascicular matrix. Together with previous work demonstrating that endogenous tendon CD146+ cells migrate to sites of injury, our data suggest that their mobilisation to promote intrinsic repair involves changes in their relationships with local interfascicular matrix vascular and basement membrane constituents.
A novel class of C14-sulfonate-tetrandrine derivatives as potential chemotherapeutic agents for hepatocellular carcinoma
Authors: Jiang Taibai , Xie Guangtong , Zeng Zhirui , Lan Junjie , Liu Hanfei , Li Jinyu , Ren Hai , Chen Tengxiang , Pan Weidong 
Published: January 2023

Hepatocellular carcinoma (HCC), the most common malignancy of the liver, exhibits high recurrence and metastasis. Structural modifications of natural products are crucial resources of antitumor drugs. This study aimed to synthesize C-14 derivatives of tetrandrine and evaluate their effects on HCC. Forty C-14 sulfonate tetrandrine derivatives were synthesized and their in vitro antiproliferative was evaluated against four hepatoma (HepG-2, SMMC-7721, QGY-7701, and SK-Hep-1) cell lines. For all tested cells, most of the modified compounds were more active than the lead compound, tetrandrine. In particular, 14-O-(5-chlorothiophene-2-sulfonyl)-tetrandrine (33) exhibited the strongest antiproliferative effect, with half-maximal inhibitory concentration values of 1.65, 2.89, 1.77, and 2.41 μM for the four hepatoma cell lines, respectively. Moreover, 33 was found to induce apoptosis via a mitochondria-mediated intrinsic pathway via flow cytometry and western blotting analysis. In addition, colony formation, wound healing, and transwell assays demonstrated that 33 significantly inhibited HepG-2 and SMMC-7721 cell proliferation, migration, and invasion, indicating that it might potentially be a candidate for an anti-HCC therapy in the future.
Gαq modulates the energy metabolism of osteoclasts
Authors: Chakraborty Sushmita , Handrick Bianca , Yu Dayoung , Bode Konrad A. , Hafner Anna , Schenz Judith , Schaack Dominik , Uhle Florian , Tachibana Taro , Kamitani Shigeki , Vogl Thomas , Kubatzky Katharina F. 
Published: January 2023

IntroductionThe bacterial protein toxinPasteurella multocidatoxin (PMT) mediates RANKL-independent osteoclast differentiation. Although these osteoclasts are smaller, their resorptive activity is high which helps in efficient destruction of nasal turbinate bones of pigs.MethodsThe proteome of bone marrow-derived macrophages differentiated into osteoclasts with either RANKL or PMT was analysed. The results were verified by characterizing the metabolic activity using Seahorse analysis, a protein translation assay, immunoblots, real-time PCR as well as flow cytometry-based monitoring of mitochondrial activity and ROS production. A Gαq overexpression system using ER-Hoxb8 cells was used to identify Gαq-mediated metabolic effects on osteoclast differentiation and function.ResultsPMT induces the upregulation of metabolic pathways, which included strong glycolytic activity, increased expression of GLUT1 and upregulation of the mTOR pathway. As OxPhos components were expressed more efficiently, cells also displayed increased mitochondrial respiration. The heterotrimeric G protein Gαq plays a central role in this hypermetabolic cell activation as it triggers mitochondrial relocalisation of pSerSTAT3 and an increase in OPA1 expression. This seems to be caused by a direct interaction between STAT3 and OPA1 resulting in enhanced mitochondrial respiration. Overexpression of Gαq mimicked the hypermetabolic phenotype observed for PMT-induced osteoclasts and resulted in higher glycolytic and mitochondrial activity as well as increased bone resorptive activity. In addition, rheumatoid arthritis (RA) patients showed an increase inGNAQexpression, especially in the synovial fluid.DiscussionOur study suggests that Gαq plays a key role in PMT-induced osteoclastogenesis. Enhanced expression ofGNAQat the site of inflammation in RA patients indicates its pathophysiological relevance in the context of inflammatory bone disorders.
In vitro leishmanicidal effect of Yangambin and Epi-yangambin lignans isolated from Ocotea fasciculata (Nees) Mez
Authors: Rebouças-Silva Jéssica , Santos Gabriel Farias , Filho José Maria Barbosa , Berretta Andresa A. , Marquele-Oliveira Franciane , Borges Valéria M. 
Published: January 2023

IntroductionYangambin and epi-yangambin are the main lignans found in Louro-de-Cheiro [Ocotea fasciculata (Nees) Mez], a tree native to the Atlantic forests of northeastern Brazil whose leaves and bark are widely used in folk medicine. The present study investigated the leishmanicidal and immunomodulatory effects of both lignans in in vitro models of infection by Leishmania amazonensis or Leishmania braziliensis, both etiological agents of Cutaneous Leishmaniasis in Brazil.MethodsBone marrow-derived mouse macrophages were infected with L. amazonensis or L. braziliensis and then treated for 48 h at varying concentrations of yangambin or epi-yangambin.ResultsYangambin and epi-yangambin were found to reduce the intracellular viability of either Leishmania species in a concentration-dependent manner, with respective IC50 values of: 43.9 ± 5 and 22.6 ± 4.9 µM for L. amazonensis, compared to IC50 values of 76 ± 17 and 74.4 ± 9.8 µM for L. braziliensis. In this context, epi-yangambin proved more selective and effective against in vitro infection by L. amazonensis. However, both lignans were found to distinctly modulate the production of inflammatory mediators and other cytokines by macrophages infected by either of the Leishmania species evaluated. While yangambin increased the production of IL-10 by L. braziliensis-infected macrophages, both compounds were observed to lower the production of NO, PGE2, IL-6 and TNF-α in both Leishmania species.DiscussionThe present results serve to encourage the development of novel studies aimed at screening natural bioactive compounds with the hope of discovering new therapeutic options for the treatment of Cutaneous Leishmaniasis.
The association between denture use and cardiovascular diseases. The United States National Health and Nutrition Examination Survey 2009–2018
Authors: Liang Xiaopeng , Chou Oscar Hou In , Cheung Bernard M. Y. 
Published: January 2023

IntroductionPoor dental health is associated with cardiovascular diseases (CVD). However, the relationship between CVD and denture use is currently unknown. This study aimed to investigate whether denture use is associated with CVD among American adults.Methods10,246 non-pregnant subjects aged 30–59 years from five cycles (2009–2018) of the United States National Health and Nutrition Examination Survey (NHANES) were included in this study. Participants who were observed by a dental examiner wearing denture/partial denture/plates were defined as denture users. CVD was defined as self-reported coronary heart disease, myocardial infarction, angina pectoris, stroke, and congestive heart failure. The association between denture use and CVD was analyzed using logistic regression with adjustment for potential cofounders.Results4.4% (95% CI, 3.9–5.0) participants had CVD, and 3.5% (95% CI, 2.8–4.5) participants were denture users. Denture use was associated with CVD [OR = 4.26, 95% CI (2.90–6.28), P &lt; 0.01], which remained significant [adjusted OR = 1.82, 95% CI (1.15–2.88), P &lt; 0.01] after adjustments for sociodemographic characteristics, smoking, alcohol use, drug addiction, body mass index (BMI), and abnormal medical conditions including gum problem, hypertension, diabetes, and hyperlipidemia. Women with dentures had significantly higher odds of CVD [adjusted OR = 2.13, 95% CI (1.10–4.11), P = 0.025].ConclusionIn this nationally representative survey, denture use was associated with CVD. Denture use may be an unconventional risk factor for assessing CVD risks, especially in women. Future studies are required to investigate whether CVD and denture use is causally related.
Amiodarone vs. metoprolol succinate in HFrEF complicated with persistent atrial fibrillation with rapid ventricular response: A prospective observational study
Authors: Liu Yongrong , Hong Yali 
Published: January 2023

Backgroundβ-blockers have been recommended for patients with heart failure (HF) and atrial fibrillation (AF), but studies have shown that β-blockers do not reduce all-cause mortality or cardiovascular mortality in patients with HF and AF.ObjectiveTo investigate the difference in efficacy between oral amiodarone and metoprolol succinate for patients with HF with reduced ejection fraction (HFrEF) and persistent atrial fibrillation (pAF) with rapid ventricular response (RVR).MethodsPatients with HFrEF complicated with pAF with RVR treated in the People's Hospital of Chongqing Hechuan between March 2018 and March 2019 were enrolled in this prospective observational study. The primary outcomes were cardiovascular mortality and the first hospitalization for HF rate. The secondary outcomes were type B pro-brain natriuretic peptide (NT-proBNP) before/after treatment, left ventricular ejection fraction (LVEF) before/after treatment, average heart rate (AhR), and the rate of sinus rhythm after 1 year of follow-up.ResultsA total of 242 patients with HFrEF complicated with pAF with RVR were enrolled and divided into amiodarone + perindopril + spironolactone+ routine drug (amiodarone group, n = 121) and metoprolol succinate + perindopril + spironolactone +routine drug (metoprolol succinate group, n = 121) according to their treatment strategy. Cardiovascular mortality (4.9 vs. 12.4%, HR: 2.500, 95%CI: 1.002–6.237, P = 0.040) and first hospitalization for HF (52.9 vs. 67.8%, HR: 1.281, 95%CI: 1.033–1.589, P = 0.024) were significantly lower in the amiodarone group than in the metoprolol group. The mean ventricular rate in the amiodarone group was significantly lower than in the metoprolol group (64.5 ± 3.2 vs. 72.4 ± 4.2, P &lt; 0.001). After 1 year of follow-up, the sinus rhythm rate was significantly higher in the amiodarone group than in the metoprolol group (38.8 vs. 7.4%, HR: 0.191, 95%CI: 0.098–0.374, P &lt; 0.001). The difference in proBNP (3,914.88 vs. 2,558.07, P &lt; 0.001) and LVEF (−6.89 vs. −0.98, P &lt; 0.001) before and after treatment was significantly higher in the amiodarone group than in the metoprolol group.ConclusionIn conclusion, in this prospective observational study, the amiodarone group had lower risk of cardiovascular death and the first hospitalization for HF than metoprolol in HFrEF and persistent atrial fibrillation (pAF) with RVR. The mechanism may be related to improved cardiac function, rhythm control and ventricular rate control.Registration numberChiCTR2200057816; Registered 7 March 2022–Retrospectively registered: http://www.medresman.org.cn/pub/cn/proj/projectshshow.aspx?proj=4222.
Secondary prevention of antithrombotic therapy in patients with stable cardiovascular disease at high ischemic risk: A network meta-analysis of randomized controlled trials
Authors: Zhu Houyong , Xu Xiaoqun , Wang Hanxin , Chen Qilan , Fang Xiaojiang , Zheng Jianwu , Gao Beibei , Tong Guoxin , Zhou Liang , Chen Tielong , Huang Jinyu 
Published: January 2023

AimsAntithrombotic secondary prevention in stable cardiovascular disease (SCVD) patients at high ischemic risk remains unclear. We compared the efficacy and safety of aspirin monotherapy, clopidogrel monotherapy, ticagrelor monotherapy, rivaroxaban monotherapy, clopidogrel plus aspirin, ticagrelor plus aspirin, and rivaroxaban plus aspirin in the high-risk ischemic cohorts.Methods and resultsEleven randomized controlled trials were included (n = 111737). The primary outcomes were major cardiovascular and cerebrovascular events (MACEs) and major bleeding. A random effects model was used for frequentist network meta-analysis. Odds ratio (OR) and 95% credible intervals (CI) were reported as a summary statistic. Compared with aspirin monotherapy, rivaroxaban plus aspirin [OR 0.79 (95% CI, 0.69, 0.89)], ticagrelor plus aspirin [0.88 (0.80, 0.98)], clopidogrel plus aspirin [0.56 (0.41, 0.77)] were associated with a reduced risk of MACEs, but rivaroxaban monotherapy [0.92 (0.79, 1.07)], ticagrelor monotherapy [0.68 (0.45, 1.05)], and clopidogrel monotherapy [0.67 (0.43, 1.05)] showed no statistically significant difference. However, rivaroxaban monotherapy and all dual antithrombotic strategies increased the risk of major bleeding to varying degrees, with ticagrelor plus aspirin associated with the highest risk of major bleeding. The net clinical benefit favored clopidogrel or ticagrelor monotherapy, which have a mild anti-ischemic effect without an increase in bleeding risk.ConclusionThe present network meta-analysis suggests that clopidogrel or ticagrelor monotherapy may be recommended first in this cohort of SCVD at high ischemic risk. But clopidogrel plus aspirin or rivaroxaban plus aspirin can still be considered for use in patients with recurrent MACEs.