bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient plasmid transfer via natural competence in a microbial co‐culture
Authors: Cheng Yu‐Yu , Zhou Zhichao , Papadopoulos James M , Zuke Jason D , Falbel Tanya G , Anantharaman Karthik , Burton Briana M , Venturelli Ophelia S 
Published: January 2023

AbstractThe molecular and ecological factors shaping horizontal gene transfer (HGT) via natural transformation in microbial communities are largely unknown, which is critical for understanding the emergence of antibiotic‐resistant pathogens. We investigate key factors shaping HGT in a microbial co‐culture by quantifying extracellular DNA release, species growth, and HGT efficiency over time. In the co‐culture, plasmid release and HGT efficiency are significantly enhanced than in the respective monocultures. The donor is a key determinant of HGT efficiency as plasmids induce the SOS response, enter a multimerized state, and are released in high concentrations, enabling efficient HGT. However, HGT is reduced in response to high donor lysis rates. HGT is independent of the donor viability state as both live and dead cells transfer the plasmid with high efficiency. In sum, plasmid HGT via natural transformation depends on the interplay of plasmid properties, donor stress responses and lysis rates, and interspecies interactions.
Cytotoxic T-lymphocyte-associated protein 4 +49A/G polymorphism in Down syndrome children with Hashimoto’s thyroiditis
Authors: Faizi Muhammad , Rochmah Nur , Hisbiyah Yuni , Perwitasari Rayi Kurnia , Putri Qurrota Ayuni Novia , Basuki Sukmawati , Endaryanto Anang , Soetjipto Soetjipto 
Published: January 2023

We aimed to evaluate the cytotoxic T-lymphocyte-associated protein 4 +49A/G (CTLA-4 +49A/G) gene polymorphism in Down syndrome (DS) children with Hashimoto’s thyroiditis (HT). This case–control study, conducted from February 2020 to February 2022 at Dr. Soetomo General Hospital, Surabaya, enrolled 40 DS children with HT and 50 healthy children. The DNA sequencing was performed to identify the polymorphism (Sanger sequencing). Thyroid peroxidase antibodies (TPOAb), thyroglobulin antibodies (TgAb), thyroid-stimulating hormone (TSH), and free thyroxine (FT4) levels were analyzed by enzyme-linked immunosorbent assay (ELISA). The mean age of DS children with HT was 1.78 years. Males predominated in the study population. Subjects with GG genotype were diagnosed earliest with hypothyroidism (8 months) compared with other studies. The most common thyroid dysfunction was central hypothyroidism, with TgAb positivity present in all patients. The AA genotype (odds ratio [OR] 0.265, 95% confidence interval [CI] 0.094–0.746; P = 0.012) and A allele (OR 0.472, 95% CI 0.309–0.721; P = 0.0002) were significantly more frequent in the control group. The AG genotype (OR 2.65, 95% CI 0.094–0.746; P = 0.003) and G allele (OR 2.116, 95% CI 1.386–3.23; P = 0.003) were more frequent in the DS with HT group. The age of the subjects in this study was younger than in previous studies. The AG genotype and the G allele were more prevalent in the DS with HT group and may be a risk factor in HT development in DS children. Furthermore, the AA genotype may act as a protective factor against HT in DS children.
Adaptation by Men to the Nurse Role. "Being Craftsmen in the Construction of their Professional Trajectory"
Authors: Velásquez Vergara Sandra Milena , Moreno Fergusson María Elisa , Arroyave Álvarez Edgar Orlando , Cacante Caballero Jasmín Viviana 
Published: January 2023

Objective. This work sought to describe the adaptation process by men to the nurse role.
Methods. Secondary analysis of data from a collective case study that had as participants 12 male nurses working in the city of Medellín, with ages between 28 and 47 years and average time of professional experience of 11 years. Information collection was carried out through in-depth interviews. The analysis was conducted through Roy’s Adaptation Model (RAM), reading of the interviews, identification of RAM’s components, grouping of fragments, assignment of tags, construction of a matrix and classification.
Results. The analysis performed accounts for the coping processes and adaptation by male nurses and the ineffective responses (control of emotions and emotional silencing) when practicing a role considered feminine.
Conclusion. In this study, it was possible to establish that, to achieve adaptation within nursing, men use strategies related with changes in bodily appearance, management of physical strength, and management of emotions.
Reflections on nursing students’ fear and anxiety arising from clinical practicums
Authors: Cruz Araújo Agostinho Antônio , De Godoy Simone , Arena Ventura Carla Aparecida , Rodolfo Silva Ítalo , Santos de Almeida Emerson Willian , Costa Mendes Isabel Amélia 
Published: January 2023

Background. Anxiety and fear are emotional responses that may emerge when individuals anticipate threats. Undergraduate nursing students may experience feelings of hopelessness and anguish in the clinical learning experience, directly impacting their academic performance. This study aims to reflect upon the fear and anxiety faced by nursing students during clinical training.
Synopsis of Contents. Two thematic axes were focused: Students’ perception regarding preceptorship attitudes and positions; Relational teaching-learning processes and their influence on the students’ professional identity. Preceptors are expected to encourage the establishment and maintenance of good relationships in the collaborative network in which students are included, especially with the multi-professional health team, to have more comprehensive academic support.
Conclusion. The role and importance of each individual in academic training, such as students and professors, is emphasized, seeking to promote positive experiences in the teaching-learning process to enable undergraduate students to more effectively develop moral sensitivity and take responsibility for patient-centered care.
MICROBIAL BIOTRANSFORMATION OF (R)-(+)- LIMONENE BY <I>Penicillium digitatum</I> DSM 62840 FOR PRODUCING (R)-(+)-TERPINEOL
Authors: PRIETO V. Gloria A. , PEREA Janeth A. , ORTIZ L. Claudia C. 
Published: January 2023

Microbial biotransformation is a relevant strategy to obtain high added value natural compounds under
controlled environmentally friendly conditions. In this research work, the biotransformation of (R)-(+)-
limonene using Penicillium digitatum DSM 62840 was evaluated. The study variables were the following:
culture medium, pH, microorganism growth phase, substrate concentration, and inducing effect of the
substrate. The results showed that a concentration of 14.7 mM limonene in the medium named malt
yeast broth at a pH of 3.5, inoculated with induced spores at the early stage of the exponential growth,
produces a high specificity and the highest concentration (1864 mg/L) of (R)-(+)-α-terpineol. The
product obtained has a considerable potential industrial application.
PRELIMINARY PHYTOCHEMISTRY ASSESSMENT OF <I> Heliconia rostrata </I> AND THE POTENTIAL INHIBITORY ACTIVITY OF SOME EFFECTS OF THE <I> Bothrops asper </I> (mapanà X) VENOM.
Authors: ESTRADA G. Sebastián , QUINTANA Juan C. , JIMENÉS Silvia L. , Alarcón P. Juan C. , PEREAÑEZ J. Jaime A. , VARGAS Leidy J. 
Published: January 2023

Some Heliconiaceae family species, had demonstrated to be effective neutralizing partial or totally the lethal, indirect hemolytic, hemorrhagic, proteolytic, clotting, miotoxic and edema-forming activities from the Bothrops asper (mapaná equis) venom. This work evaluates two species of heliconias: Heliconia psittacorum and Heliconia rostrata against the indirect hemolytic, proteolytic and clotting activities induced by B. asper without any inhibitory effect on the indirect hemolytic and proteolytic effects. On the contrary, the extracts retard the venom clotting effect with regard to the positive control up to 45.59 seconds. In the electrophoretic profile a band belonging to the H. psittacorum is observed, moreover a decrease and increase in the intensity of some bands (66 y 31 KDa respectively) possibly indicate its degradation by the extracts. The phytochemical evaluation of the extracts shows the presence of primary metabolites as glucids and proteins; and secondary metabolites as phenolics compounds, terpenoides and cumarins, some of them reported in others vegetal species as responsible of the anti-ophidian activity.
Level of self-care practices and associated factors among Hypertensive Patients in Addis Ababa, Ethiopia
Authors: Sahile Addisu Tadesse , Nurhussien Hayat Abdulkadir 
Published: January 2023

Abstract
Objectives: The study assessed the level of self-care practice and its predictors among hypertensive patients in the health centers of Bole Sub-city, Addis Ababa, Ethiopia.
Methods: A multi-Center-based cross-sectional study that employed 370 hypertensive participants at the conveniently selected Health Centers in Bole Sub-City; conducted from August 01-30, 2020. The researchers selected the participants based on a simple random sampling method after obtained with a written informed consent and applying a pre-tested interviewer-administered questionnaire for data collection. All the statistical analyses were done using SPSS 22.0 software. Binary logistics regression used to determine the presence and strength of association; with its respective 95%CI and p-value, less than 5% used as a significant level.
Results: The overall level of good self-care practice among hypertensive patients was 53.0 %( 95%CI: 47.2%-58.8%) whereas 61.4%, 63.8%, 92.7%, 82.7%, and 18% of the study participants were adherent to medication, good weight management, non-smokers, alcohol abstainers and physical activity consecutively.
Being illiterate had 2.347 and 2.084 times higher odds of having had good self-care practice compared to secondary school and diploma or above consecutively. Being a merchant, civil, and retired were associated with good self-care practice than being unemployed.
Conclusion and Recommendation: A lower level of self-care practice was observed. Educational level and occupation were factors identified for self-care practice. The policymakers, healthcare workers, and researchers were recommended to work on the identified factors of self-care practice of hypertensive participants in the study settings.
Paleo–climatic and paleo–environment implication of clay mineral across Upper Cretaceous­–Lower Eocene deposits from Gafsa Basin
Authors: Majid Abdel 
Published: January 2023

Abstract
An integrated study of clay paragenesis has been conducted in the Gafsa Basin, southern Tunisia, during the Late Cretaceous–Lower Eocene. Six lithostratigraphic sections were logged in the Tamerza, Oued Thelja, Ong, and Chamsi. The Upper Maastrichtian Haria Formation is dominated by smectite, indicating a semi-arid climate with distinct seasons. A colder and drier climate, favoring physical alteration, is observed in the first Paleogene deposits. Danian deposits, show a decrease in smectite in favor of illite and kaolinite, indicating a period of intensified detritus under a hot and humid climate with a contrasting season. The abundance of smectite and the low percentages of kaolinite in the Thelja (Thanetian) Formation suggest a warm and arid climate. The late Thanetian–early Eocene deposits were influenced by a warm and seasonal climate, or probably, they were an alternation between dry and humid seasons and warm climatic conditions in the Lower Eocene corresponding to the Late Paleocene–early Eocene deposits outlining the Paleocene–Eocene Thermal Maximum (PETM). The consequence of this warm climate is sea level rise, which corresponds to the Paleocene– Eocene transgression.
Parents' awareness of the conscious use of applications on smart devices: A scale development study
Authors: Öztürk Gülcan , Şahin Feyza 
Published: January 2023

Abstract
Children inevitably start using smart devices from the first year of their lives. Parents should have knowledge and awareness of the conscious use of applications on smart devices. For this reason, a scale to measure parents’ knowledge and awareness of the conscious use of applications on smart devices has become necessary. Due to this necessity, we want to conduct this study. The research’s problem was how parents’ knowledge and awareness of the conscious use of applications on smart devices could be measured using a valid and reliable scale. We conducted the research in an exploratory sequential design with two stages one of the mixed methods research. We collected and analyzed qualitative data from 33 parents in the first stage, and we collected quantitative data from 602 parents and performed a quantitative analysis to evaluate the first stage’s results in the second stage. After reviewing the literature, we concluded that the scale’s dimensions as a theoretical model were (1) application, (2) benefit, (3) restriction, and (4) worry. We decided on the scale’s items according to the theoretical model after analyzing the parents’ opinions and obtaining experts’ evaluations for the content validity of the scale items. We demonstrated that the theoretical model was supported by the analyses we performed using the data we collected. We learned how a valid and reliable scale could be used to measure the parents’ knowledge and awareness of the conscious use of applications on smart devices, including current and future computer systems. The scale we developed had more specific dimensions compared to the scales in the literature about digital parenting.
Reporting Quality of Randomized Controlled Trials Evaluating Non- Vitamin K Oral Anticoagulants in Atrial Fibrillation：A systematic review
Authors: Yang YueGuang , Han YuBo , Zou GuoLiang , Sui YanBo , Jin Juan , Liu Li 
Published: January 2023

Abstract
Background: Randomized controlled trials (RCTs) are subject to bias if they lack methodological quality. Furthermore, optimal and transparent reporting of RCT ﬁndings aids their critical appraisal and interpretation. This study aimed to comprehensively evaluate the report quality of RCTs of non-vitamin K oral anticoagulants (NOACs) for the treatment of atrial fibrillation (AF) and to analyze the factors influencing the quality.
Methods: By searching PubMed, Embase, Web of Science, and Cochrane Library databases RCTs published from inception to 2022 evaluating the efficacy of NOACs on AF were collected. By using the 2010 Consolidated Standards for Reporting Tests (CONSORT) statement, the overall quality of each report was assessed.
Results: Sixty-twoRCTs were retrieved in this study. The median of overall quality score in 2010 was 14 (range: 8.5-20). The extent of compliance with the Consolidated Standards of Reporting Trials reporting guideline differed substantially across items: 9 items were reported adequately (more than 90%), and 3 were reported adequately in less than 10% of trials. Multivariate linear regression analysis showed that the higher reporting scores were associated with higher journal impact factor (P = 0.02), international collaboration (P < 0.01), and Sources of trial funding (P = 0.04).
Conclusions: Although a large number of randomized controlled trials of NOACs for the treatment of AFwere published after the CONTORT statement in 2010, the overall quality is still not satisfactory, thus weakening their potential utility and may mislead clinical decisions. This survey provides the first hint for researchers conducting trials of NOACs for AF to improve the quality of reports and to actively apply the CONSORT statement.
Bone marrow mesenchymal stem cell exosomes attenuate neuroinflammation after diabetic intracerebral hemorrhage via miR-129-5p/HMGB1
Authors: wang yueying , li ke , zhu hong , liu zhiyi , wang jiajun , hua wei , liu qi , sun yulan , zhang qianqian , song yuejia , qi jiping 
Published: January 2023

Abstract
Background
In this study, we investigated whether mesenchymal stem cell (MSC)-derived exosomes (Exos) could regulate hyperglycemia-induced neuroinflammation by transferring microRNA-129-5p (miR-129-5p).
Methods
Bone marrow-derived MSC (BMSC)-Exos were isolated from media BMSCs from mice. This was followed by transfection with miR-129-5p. BMSC-Exos or miR-129-5p-overexpressing BMSC-Exos were intravitreally injected into diabetic mice with intracerebral hemorrhage (DM/ICH) for in vivo analyses and were cocultured with high glucose-affected BV2 cells for in vitro analyses. RT-PCR and western blotting were conducted to assess the levels of some inflammation factors, such as high-mobility group box 1 (HMGB1), interleukin (IL)-6, IL-1β, toll-like receptor 4 (TLR4), and tumor necrosis factor α (TNFα).
Results
Our results indicate that hyperglycemia considerably increased inflammation in ICH mice or in BV2 cells exposed to high glucose with hemin and increased the expression levels of hmgb1 and its downstream inflammatory factors. BMSC-Exos administration could effectively reverse this reaction. Compared with administration of BMSC-Exos, administration of miR-129-5p-overexpressing BMSC-Exos more effectively suppressed the HMGB1 signaling pathway and suppressed inflammation both in vivo and in vitro. We also observed an improvement in cerebral hematoma and cerebral edema in DM/ICH mice after administration of the miR-129-5p mimic, which reduced the permeability of the blood–brain barrier.
Conclusions
We demonstrated that BMSC-Exos can reduce the inflammatory response after DM/ICH, thereby improving the neurological function of the brain, and we demonstrate that their effect is achieved through the miR-129-5p/HMGB1/TLR4 regulatory axis. At present, the mechanism by which exosomes from bone marrow mesenchymal stem cells regulate neuroinflammation after diabetic cerebral hemorrhage remains unclear. The results of this study provide a theoretical basis for the recovery of neurological function after diabetic cerebral hemorrhage and may provide a new targeted therapy for neuroinflammation.
Multi-omics data-based analysis characterizes molecular alterations of the vesicle genes in human colorectal cancer
Authors: Wang Xi , Wu Minmin , Zhang Wei , Liu Zhenqiong , Ding Yang , Xu Mengqi , Zhang Fengmei , He Zhiqiang , Tang Min , Tang Donge , Dai Yong 
Published: January 2023

Abstract
Background: Vesicular genes are crucial to the development of colorectal cancer. Understanding the molecular pathways behind colorectal carcinogenesis and identifying possible treatment targets can be accomplished by analyzing alterations in vesicle genes at multi-omics. Studies on the overall alteration of vesicle genes in colorectal cancer are still lacking, nevertheless.
Methods: In order to identify a potential association between vesicle genetic alterations and CRC progression, we analyzed molecular alterations in CRC vesicle genes at eight levels in this study, including mRNA, protein, and epigenetic levels. We also analyzed CRC overall survival related genes that were obtained from public database.
Results: The analysis of the chromatin structural variants, DNA methylation, chromatin accessibility, proteins, protein phosphorylation, ubiquitination, and malonylation of our collected CRC tissues in combination with the RNA-seq data from the TCGA database revealed the presence of multiple levels of alterations in CRC vesicle genes. We progressively examined the alterations of vesicle genes in mRNA and protein levels in CRC and discovered the hub genes COL5A1 and HSPA8. Further investigation identified the probable essential transcription factors YY1, CDX2, and CBX3.
Conclusions: This study contributes to a thorough knowledge of the connection between vesicle genes alterations in multiple level and the development of CRC and offers a theoretical framework for the identification of novel treatment targets.
A new fractional Cattaneo model for enhancing the thermal performance of Photovoltaic panels using heat spreader: Energy, exergy, economic and enviroeconomic (4E) analysis
Authors: El-Gazar Eman , Hassan Hamdy , Rabia Sherif , HU Changhong , Zahra Waheed 
Published: January 2023

AbstractA new fractional non-Fourier (Cattaneo) photovoltaic (PV) model is developed to improve the thermal performance of a PV system combined with a heat spreader (HS). In contrast to the traditional Fourier model, which is unable to reliably predict system performance, the fractional Cattaneo model proves its efficiency in looking at transient processes throughout the entire PV system. It can also accurately expect PV performance even at short temporal scales. Consequently, a comparison is conducted between the classical Fourier model with the fractional Fourier and fractional Cattaneo models for the PV system. Real experimental data are used to validate the fractional Cattaneo model. The findings show that it perfectly matches the measured data, with an error percentage in PV power and exergy efficiency of only 0.628% and 3.84%, respectively, compared to 5.72% and 13.13% resulted from the classical model. An assessment of the effect of using rectangular and trapezoidal spreaders on output power, electrical efficiency, exergy efficiency, economic, exergoeconomic, and enviroeconomic parameters is introduced and discussed. The outcomes revealed that the electrical production costs of the rectangular and trapezoidal HS systems are 0.272 and 0.214 $/kWh, respectively, while about 0.286 $/kWh for the conventional PV panel. Finally, the findings show that the modified PV systems with the spreader are liked to be greener than the conventional ones.
Thermo economic assessment of photovoltaic/thermal panels-powered RO desalination unit combined with preheating using geothermal energy
Authors: Bacha Habib Ben , Abdullah Abdelkader Saad , Abdelgaied Mohamed , Kabeel Abd Elnaby 
Published: January 2023

Abstract
Recently, the reverse osmosis process is widely used in the field of desalinating brackish water and seawater to produce freshwater, but the disadvantage of using this technology is the increase in the rates of electrical energy consumption necessary to manage these units. To reduce the rates of electrical energy consumption in the RO desalination plants, geothermal energy was used as pre-heating system to heat feed water before entering RO desalination plants. The proposed system in this study consists of RO desalination plant with an energy recovery device, photovoltaic/thermal panels, and a geothermal energy extraction unit. To evaluate the system performance, three incorporated models were studied and validated by the previous experimental data. The results indicated that incorporating the geothermal energy and photovoltaic/thermal panels with the RO desalination plants has positive effects in terms of increasing productivity and reducing the rates of specific power consumption in RO desalination plants. The average saving in the specific power consumption for utilizing the thermal recovery system of PV panels and geothermal energy as preheating units reached 29.1% and 40.75% for treatment seawater and brackish water, respectively. Also, the economic feasibility showed that the saving the in the cost of freshwater produced from the RO desalination plants for incorporating both geothermal energy and photovoltaic panels with a thermal recovery system with reverse osmosis desalination plants up to 39.6%.