bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-complement factor H (CFH) autoantibodies could delay pristane-induced lupus nephritis
Authors: Li Lin-Lin , Luan Zhong-qiu , Tan Ying , Wang Hui , Yu Xiao-Juan , Qu Zhen , Yu Feng , Chen Min 
Published: June 2023

Abstract
Purpose
Anti-complement factor H (CFH) autoantibodies could be detected in lupus and its significance remained to be elucidated. Herein, we aimed to explore the roles of anti-CFH autoantibodies based on pristane-induced lupus mice.

Methods
Twenty-four female Balb/c mice were randomly divided into four groups, with one group injected with pristane (pristane group), one group with pristane and then human CFH (hCFH) (pristane-CFH group) 3 times, and the other two as vertical controls, PBS group and PBS-CFH group. Histopathological analysis was performed six months after pristane administration. Levels of hCFH, anti-CFH autoantibodies and anti-dsDNA antibody were detected. Murine IgG (mIgG) were purified and cross-reactivity, epitopes, subclasses and functional analysis were further evaluated in vitro.

Results
Immunization with hCFH and subsequent development of anti-CFH autoantibodies significantly attenuated nephritis of pristane-induced lupus, including lower levels of urinary protein and serum creatinine, decreased levels of serum anti-dsDNA antibody, greatly ameliorated renal histopathologic damage, decreased IgG, complements (C1q, C3) deposits and lower inflammatory factor (IL-6) expression in glomerulus. Furthermore, the purified mIgG (contained anti-CFH autoantibodies) could recognize both hCFH and murine CFH, and the epitopes were predominantly located in hCFH short consensus repeats (SCRs) 1–4, 7 and 11–14. The IgG subclasses were predominant IgG1. The autoantibodies could enhance the binding between hCFH and C3b, and increase factor I mediated-C3b lysis in vitro.

Conclusion
Our results suggested that anti-CFH autoantibodies could attenuate pristane-induced lupus nephritis by increasing bio-functions of CFH on regulating complement activation and controlling inflammation.
Molecular mechanisms of syncytin-1 in tumors and placental development related diseases
Authors: Wang Qianqian , Shi Ying , Bian Qiang , Zhang Naibin , Wang Meng , Wang Jianing , Li Xuan , Lai Luhao , Zhao Zhankui , Yu Honglian 
Published: June 2023

AbstractHuman endogenous retroviruses (HERVs) have evolved from exogenous retroviruses and account for approximately 8% of the human genome. A growing number of findings suggest that the abnormal expression of HERV genes is associated with schizophrenia, multiple sclerosis, endometriosis, breast cancer, bladder cancer and other diseases. HERV-W env (syncytin-1) is a membrane glycoprotein which plays an important role in placental development. It includes embryo implantation, fusion of syncytiotrophoblasts and of fertilized eggs, and immune response. The abnormal expression of syncytin-1 is related to placental development-related diseases such as preeclampsia, infertility, and intrauterine growth restriction, as well as tumors such as neuroblastoma, endometrial cancer, and endometriosis. This review mainly focused on the molecular interactions of syncytin-1 in placental development-related diseases and tumors, to explore whether syncytin-1 can be an emerging biological marker and potential therapeutic target.
The immunomodulatory function and antitumor effect of disulfiram: paving the way for novel cancer therapeutics
Authors: Zhang Sijia , Zong Yan , Chen Leichong , Li Qianwen , Li Zhenyu , Meng Rui 
Published: June 2023

AbstractMore than 60 years ago, disulfiram (DSF) was employed for the management of alcohol addiction. This promising cancer therapeutic agent inhibits proliferation, migration, and invasion of malignant tumor cells. Furthermore, divalent copper ions can enhance the antitumor effects of DSF. Molecular structure, pharmacokinetics, signaling pathways, mechanisms of action and current clinical results of DSF are summarized here. Additionally, our attention is directed towards the immunomodulatory properties of DSF and we explore novel administration methods that may address the limitations associated with antitumor treatments based on DSF. Despite the promising potential of these various delivery methods for utilizing DSF as an effective anticancer agent, further investigation is essential in order to extensively evaluate the safety and efficacy of these delivery systems.
“What if” should precede “whether” and “how” in the social conversation around human germline gene editing
Authors: Houtman Diewertje , Geuverink Wendy , Helmrich Isabel Rosalie Arianne Retel , Vijlbrief Boy , Cornel Martina , Riedijk Sam 
Published: June 2023

AbstractGiven the potential large ethical and societal implications of human germline gene editing (HGGE) the urgent need for public and stakeholder engagement (PSE) has been repeatedly expressed. In this short communication, we aim to provide directions for broad and inclusive PSE by emphasizing the importance of futures literacy, which is a skill to imagine diverse and multiple futures and to use these as lenses to look at the present anew. By first addressing “what if” questions in PSE, different futures come into focus and limitations that arise when starting with the “whether” or “how” questions about HGGE can be avoided. Futures literacy can also aid in the goal of societal alignment, as “what if” questions can be answered in many different ways, thereby opening up the conversation to explore a multitude of values and needs of various publics. Broad and inclusive PSE on HGGE starts with asking the right questions.
Diagnostic accuracy of upper gastrointestinal series in children with suspected intestinal malrotation
Authors: Girolamo Mattioli , Emanuela Gallo , Yu Wong Michela Cing , Anna Marzoli , Marta Pongiglione , Grazia Calevo Maria , Gandullia Paolo , Arrigo Serena , Stefano Avanzini , Beatrice Damasio Maria 
Published: June 2023

AbstractIntestinal malrotation (IM) results from an altered or incomplete rotation of the fetal midgut around the superior mesenteric artery axis. The abnormal anatomy of IM is associated with risk of acute midgut volvulus which can lead to catastrophic clinical consequences. The upper gastro-intestinal series (UGI) is addressed as the gold standard diagnosis procedure, but a variable failure degree has been described in literature. The aim of the study was to analyze the UGI exam and describe which features are the most reproducible and reliable in diagnosing IM. Medical records of patients surgically treated for suspected IM between 2007 and 2020 at a single pediatric tertiary care center were retrospectively reviewed. UGI inter-observer agreement and diagnostic accuracy were statistically calculated. Images obtained with antero-posterior (AP) projections were the most significant in terms of IM diagnosis. Duodenal-Jejunal Junction (DJJ) abnormal position resulted to be the most reliable parameter (Se = 0.88; Sp = 0.54) as well as the most readable, with an inter-reader agreement of 83% (k = 0.70, CI 0.49–0.90). The First Jejunal Loops (FJL), caecum altered position and duodenal dilatation could be considered additional data. Lateral projections demonstrated an overall low sensitivity (Se = 0.80) and specificity (Sp = 0.33) with a PPV of 0.85 and a NPV of 0.25. UGI on the sole AP projections ensures a good diagnostic accuracy. The position of the third portion of the duodenum on lateral views showed an overall low reliability, therefore it was not helpful but rather deceiving in diagnosing IM.
Environmental feasibility of recycling construction and demolition waste
Authors: Atta I. , Bakhoum E. S. 
Published: June 2023

AbstractConstruction, demolition, and renovation activities generate a significant amount of waste, posing serious environmental risks. The scarcity of recycling facilities makes it difficult to implement the new legislation, which calls for producing recycled aggregates. Moreover, the lack of studies on the environmental feasibility of recycling construction and demolition waste in regions with plentiful natural resources of aggregates is a contributing factor to this scarcity. Therefore, this paper studies the environmental feasibility of establishing a construction and demolition waste (CDW) recycling plant. A case study approach compares the CDW recycling process against the traditional quarrying process for generating aggregates. The lifecycle assessment method is used for evaluating both scenarios. Contribution analysis is performed to infer the factors influencing the viability of the recycling process. Thereafter, a sensitivity analysis is conducted to determine the suitability of the proposed location for the recycling facility. As revealed by the single score, the recycling alternative has a 23% more negative environmental impact than quarrying. Nevertheless, there is a chance that the recycling process could be environmentally advantageous. Findings indicated that a total travel distance of 70 km between the demolition site and the construction site is recommended to guarantee environmental feasibility. The transportation distance is found to be the most critical element influencing the environmental feasibility of recycling CDW. The findings of this study help the decision-makers in environmental affairs to consider the influencing factors when constructing a CDW recycling plant.
Correlation of lipocalin 2 and glycolipid metabolism and body composition in a large cohort of children with osteogenesis imperfecta
Authors: Zheng W.-b. , Hu J. , Sun L. , Liu J.-y. , Zhang Q. , Wang O. , Jiang Y. , Xia W.-b. , Xing X.-p. , Li M. 
Published: June 2023

Abstract
Purpose
Lipocalin 2 (LCN2) is a newly recognized bone-derived factor that is important in regulation of energy metabolism. We investigated the correlation of serum LCN2 levels and glycolipid metabolism, and body composition in a large cohort of patients with osteogenesis imperfecta (OI).

Methods
A total of 204 children with OI and 66 age- and gender-matched healthy children were included. Circulating levels of LCN2 and osteocalcin were measured by enzyme-linked immunosorbent assay. Serum levels of fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC), and low- and high-density lipoprotein cholesterol (LDL-C, HDL-C) were measured by automated chemical analyzers. The body composition was measured by dual-energy X-ray absorptiometry. Grip strength and timed-up-and-go (TUG) were tested to evaluate the muscle function.

Results
Serum LCN2 levels were 37.65 ± 23.48 ng/ml in OI children, which was significantly lower than those in healthy control (69.18 ± 35.43 ng/ml, P < 0.001). Body mass index (BMI) and serum FBG level were significantly higher and HDL-C levels were lower in OI children than healthy control (all P < 0.01). Grip strength was significantly lower (P < 0.05), and the TUG was significantly longer in OI patients than healthy control (P < 0.05). Serum LCN2 level was negatively correlated to BMI, FBG, HOMA-IR, HOMA-β, total body, and trunk fat mass percentage, and positively correlated to total body and appendicular lean mass percentage (all P < 0.05).

Conclusions
Insulin resistance, hyperglycemia, obesity, and muscle dysfunction are common in OI patients. As a novel osteogenic cytokine, LCN2 deficiency may be relevant to disorders of glucose and lipid metabolism, and dysfunction of muscle in OI patients.
Exploring the nonstationarity of coastal sea level probability distributions
Authors: Falasca Fabrizio , Brettin Andrew , Zanna Laure , Griffies Stephen M. , Yin Jianjun , Zhao Ming 
Published: June 2023

AbstractStudies agree on a significant global mean sea level rise in the 20th century and its recent 21st century acceleration in the satellite record. At regional scale, the evolution of sea level probability distributions is often assumed to be dominated by changes in the mean. However, a quantification of changes in distributional shapes in a changing climate is currently missing. To this end, we propose a novel framework quantifying significant changes in probability distributions from time series data. The framework first quantifies linear trends in quantiles through quantile regression. Quantile slopes are then projected onto a set of fourorthogonalpolynomials quantifying how such changes can be explained byindependentshifts in the first four statistical moments. The framework proposed is theoretically founded, general, and can be applied to any climate observable with close-to-linear changes in distributions. We focus on observations and a coupled climate model (GFDL-CM4). In the historical period, trends in coastal daily sea level have been driven mainly by changes in the mean and can therefore be explained by a shift of the distribution with no change in shape. In the modeled world, robust changes in higher order moments emerge with increasing$ {\mathrm{CO}}_2 $concentration. Such changes are driven in part by ocean circulation alone and get amplified by sea level pressure fluctuations, with possible consequences for sea level extremes attribution studies.
WHAT IS THE HARM IN ADDICTION? AUTONOMY, VULNERABILITY, AND THE CASE FOR HARM REDUCTION DRUG POLICY
Authors: Doucet Mathieu , Porter Lindsey Brooke 
Published: June 2023

AbstractSuccessful drugs policy must be driven by thoughtful principle and intergovernmental consensus, not by departmental or legal inertia, nor by public (mis)conceptions about drug use. Perhaps the most pressing choice for drugs policymakers at present is between harm reduction and abstinence approaches to drugs policy. To choose between these two approaches, we need to know addiction's normative status: is having an addiction a misfortune or a harm in its own right, even setting aside knock-on health and wellbeing consequences? We argue that the harm of addiction is driven by poor policies, but that harm is not inevitable.
NAPE VERTIT: A NOTE ON OVID, AMORES 1.12
Authors: Swain Natalie J. 
Published: June 2023

AbstractThe hairdresser who carries Ovid's invitation to his puella in Amores 1.11 is almost immediately blamed for his rejection in 1.12, before that blame is transferred to the tablets carrying that invitation. Nape (the enslaved hairdresser of the puella) has been linked to the character Dipsas, appearing in 1.7, specifically through the descriptor sobria. By focussing on the use of the verb uerto, the reference to the mythical strix, and curses related to the old age of both Dipsas and the tablets in 1.7 and 1.12, this note demonstrates that the supernatural word choice further connects Nape with Dipsas.
Baumslag–Solitar groups and residual nilpotence
Authors: Kofinas C.E. , Metaftsis V. , Papistas A.I. 
Published: June 2023

AbstractLet G be a Baumslag–Solitar group. We calculate the intersection $\gamma_{\omega}(G)$ of all terms of the lower central series of G. Using this, we show that $[\gamma_{\omega}(G),G]=\gamma_{\omega}(G)$, thus answering a question of Bardakov and Neschadim [1]. For any $c \in \mathbb{N}$, with $c \geq 2$, we show, by using Lie algebra methods, that the quotient group $\gamma_{c}(G)/\gamma_{c+1}(G)$ of the lower central series of G is finite.
Effects of time and temperature of storage on chemical and nutritional characteristics of raw milk for Provolone Valpadana PDO cheesemaking: a multivariate approach
Authors: Paggio Federico , Ritota Mena , Di Costanzo Maria Gabriella , Barzaghi Stefania , Monti Lucia , Ulrici Alessandro , Manzi Pamela 
Published: June 2023

AbstractWe evaluated the possibility of increasing the storage temperature of raw milk for Provolone Valpadana cheesemaking, to identify the most suitable conditions of time and temperature for a pre-maturation process. We used Principal Component Analysis (PCA) to analyze the overall effects of different storage conditions on chemical, nutritional and technological characteristics of the raw milk. Four different thermal storage cycles, two at fixed temperature/time (6 and 12°C for 60 h) and two with two-phase thermal cycle (10 and 12°C for 15 h, followed by refrigeration at 4°C for 45 h) were studied. Although a moderate heterogeneity among raw milks from the 11 producers of Provolone Valpadana cheese was observed, PCA revealed the critical aspects of the extreme storage conditions (60 h of refrigeration). Some samples resulted in anomalous behaviors, probably related to unexpected fermentation phenomena occurring with increasing storage temperature. The acidification and the increase in the contents of lactic acid, soluble calcium, and degree of retinol isomerization observed in the anomalous samples can compromise the technological functionality of milk. Conversely, the storage with a two-phase thermal cycle did not lead to variations in any measured characteristic, suggesting that mild refrigeration conditions (10 or 12°C for 15 h followed by 4°C for 45 h) could be a good compromise in favoring milk pre-maturation without altering its quality characteristics.
Flagella are an important virulence factor in the subclinical persistence of Escherichia coli in bovine mammary gland
Authors: Gonçalves Maysa Serpa , Faria Jamila PJ , Silva Juliana R , Custódio Dircéia AC , Ribeiro João B , Guimarães Alessandro de S , Dorneles Elaine MS , Costa Geraldo M 
Published: June 2023

AbstractWe compared the virulence profile and REP-PCR genotypes of Escherichia coli strains isolated from subclinical and clinical mastitis cases and dairy farm environments in Minas Gerais State, Brazil, to determine virulence factors and genotypes potentially associated with subclinical persistence in the udder. The virulence profile was obtained by the search for three virulence genes: lpfA (long polar fimbriae), fliC (flagella), and escN (type III secretion system). Subclinical isolates exhibited mainly the fliC gene (33.33%) and fliC + escN genes (30.30%). Clinical isolates exhibited mainly fliC + escN genes (50%) and environmental isolates the lpfA + escN genes (58.04%). Strains isolated from subclinical mastitis showed 6.75 times more positivity to fliC than environmental isolates. Thirty-four genotypes were observed in the REP-PCR analysis, and clinical mastitis isolates indicated more genetic proximity to dairy farm environment isolates than subclinical mastitis isolates. In conclusion, the results suggested that flagella may be an important virulence factor for mammary persistent E. coli infection in cattle, however, none of the E. coli REP-PCR genotypes were associated with subclinical infection.
Early life adversity predicts an accelerated cellular aging phenotype through early timing of puberty
Authors: Hamlat Elissa J. , Neilands Torsten B. , Laraia Barbara , Zhang Joshua , Lu Ake T. , Lin Jue , Horvath Steve , Epel Elissa S. 
Published: June 2023

AbstractBackgroundThe current study examined if early adversity was associated with accelerated biological aging, and if effects were mediated by the timing of puberty.MethodsIn early mid-life, 187 Black and 198 White (Mage = 39.4, s.d.age = 1.2) women reported on early abuse and age at first menstruation (menarche). Women provided saliva and blood to assess epigenetic aging, telomere length, and C-reactive protein. Using structural equation modeling, we created a latent variable of biological aging using epigenetic aging, telomere length, and C-reactive protein as indicators, and a latent variable of early abuse using indicators of abuse/threat events before age 13, physical abuse, and sexual abuse. We estimated the indirect effects of early abuse and of race on accelerated aging through age at menarche. Race was used as a proxy for adversity in the form of systemic racism.ResultsThere was an indirect effect of early adversity on accelerated aging through age at menarche (b = 0.19, 95% CI 0.03–0.44), in that women who experienced more adversity were younger at menarche, which was associated with greater accelerated aging. There was also an indirect effect of race on accelerated aging through age at menarche (b = 0.25, 95% CI 0.04–0.52), in that Black women were younger at menarche, which led to greater accelerated aging.ConclusionsEarly abuse and being Black in the USA may both induce a phenotype of accelerated aging. Early adversity may begin to accelerate aging during childhood, in the form of early pubertal timing.
Grassroots scaling up: Navigating algorithmic scales in a technopolitical landscape
Authors: Zentz Lauren 
Published: June 2023

AbstractMy theoretical aim in this article is to focus on an examination of processual enactments of scale in light of the technological affordances that are currently at the disposal of a significant majority of humans. I offer the terms algorithmic scales and algorithmic scalar affordances to describe one activist's engagement of practical theories of scale—her ‘algorithmic imagination’ (Bucher 2017)—which led her to design her audience in ways intended to algorithmically scale up, or amplify, her activities on Facebook—to enhance their spread numerically, rapidly, and translocally, making use of the algorithmically constructed communicative possibilities or affordances available to her on the site. (Social media, scale, algorithms, audience design, Facebook, activism, nation, politics)
A consensus-based checklist for the critical appraisal of cost-of-illness (COI) studies
Authors: Schnitzler Lena , Roberts Tracy E. , Jackson Louise J. , Paulus Aggie T. G. , Evers Silvia M. A. A. 
Published: June 2023

Abstract

Objectives
To develop a consensus-based checklist that can be used as a minimum standard to appraise the comprehensiveness, transparency and consistency of cost-of-illness (COI) studies. This is important when, for instance, reviewing and assessing COI studies as part of a systematic review or when building an economic model.

Methods
The development process of the consensus-based checklist involved six steps: (i) a scoping review, (ii) an assessment and comparison of the different checklists and their questions, (iii) the development of a (preliminary) checklist, (iv) expert interviews, (v) the finalization of the checklist, and (vi) the development of guidance statements explaining each question.

Results
The result was a consensus-based checklist for the critical appraisal of COI studies, comprising seventeen main questions (and some additional subquestions) across three domains: (i) study characteristics; (ii) methodology and cost analysis; and (iii) results and reporting. Guidance statements were developed describing the purpose and meaning behind each question and listing examples of best practice. The following answer categories were suggested to be applied when answering the questions in the checklist: Yes, Partially, No, Not Applicable, or Unclear.

Conclusions
The consensus-based checklist for COI studies is a first step toward standardizing the critical appraisal of COI studies and is one that could be considered a minimum standard. The checklist can help to improve comprehensiveness, transparency and consistency in COI studies, to address heterogeneity, and to enable better comparability of methodological approaches across international studies.