bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the usefulness of determining the level of selected inflammatory biomarkers and resistin concentration in perivascular adipose tissue and plasma for predicting postoperative atrial fibrillation in patients who underwent myocardial revascularisation
Authors: Rachwalik M. , Matusiewicz M. , Jasiński M. , Hurkacz M. 
Published: January 2023

Abstract
Background
The development of coronary artery disease (CAD) is related to the impaired quantity and composition of inflammatory proteins found in plasma and tissue, such as interleukin 6 (IL-6), adipokines, and resistin. Therefore, the level of plasma resistin in patients with advanced CAD could be indicative of the condition of epicardial adipose tissue and thus have an impact on the frequency and severity of postoperative complications in the form of paroxysmal atrial fibrillation.

Methods
The study included 108 patients who qualified for elective coronary artery bypass grafting (CABG) surgery from 2017 to 2020 and were categorized into two groups. The first group consisted of patients who developed atrial fibrillation in the postoperative period – the AF group, and the second group included patients who did not have arrhythmia – the non-AF group. The analysis incorporates the history, course of treatment, anthropometric characteristics of the test subjects, biochemical laboratory tests, and echocardiography. Perivascular adipose tissue (PVAT) sections were surgically harvested from the area of the left coronary trunk.

Results
The resistin levels in the PVAT were significantly higher in the AF group than in the non-AF group (P = 0.000015). Similarly, plasma resistin levels increased significantly in the AF group compared to the non-AF group (P = 0.044). The values of other analyzed variables were not significantly different (analysis performed using the Mann–Whitney U test). Spearman’s rank-order correlation technique found a correlation between resistin in PVAT and plasma (r = 0.5933; P < 0.0001) in the whole study group, as well as in the AF group (r = 0.4782; P = 0.021) and the non-AF group (r = 0.4938; P < 0.0001). A correlation arose between the level of resistin in PVAT and the level of hsCRP (r = 0.3463; P = 0.005) in the whole study group and the non-AF group (r = 0.4448; P = 0.0011); however, no such correlation appeared in the AF group (r = 0.3076; P = 0.306).

Conclusions
Elevated levels of plasma resistin, which reflect PVAT resistin levels in patients qualified for myocardial revascularisation, may be associated with postoperative atrial fibrillation complications.
Influence of climatic factors on essential oil content and composition of 20 populations of Nepeta binaludensis Jamzad from Iran
Authors: Moghaddam Hosien Hashemi , Jafari Ali Ashraf , Sefidkon Fatemeh , Jari Sepideh Kalate 
Published: January 2023

AbstractNepeta binaludensis Jamzad is an endemic and rare perennial plant belonging to the Lamiaceae family, which grows in a limited area in Binaloud Mountain in northeast of Iran. In this study to evaluate the diversity of 20 populations (localities) of N. binaludensis and the influence of environmental factors on essential oil (EO) content and composition, the plant aerial parts were collected at the full flowering stage. The plant materials dried in shade and subjected to hydro-distillation for obtaining their EOs. Analysis of the EO was carried out using GC and GC/MS. The oil yields were varied from 1.2 to 4.9%. Classification of populations was made based on EO compounds. The results of mean comparison between populations belong to different habitats showed that the populations of Darood and Friezy with average values of 4.91 and 1.2% had the highest and lowest EO yield, respectively. Twenty-two compounds were identified in the oils with 1,8-cineol (25.4–59.0%), 4aα,7α,7aα-nepetalactone (13.8–55.1%), myrcene (2.3–5.5%) and p-cymene (1.1–5.7%) as the main components. Result of correlation analysis showed that the oil yield was positively correlated with precipitation and negatively with temperature. In addition, 1,8-cineole was positively and nepetalactone was negatively correlated with altitude. Cluster analysis by Ward method categorized the populations into two groups. The major compound of the oils in cluster 1 was nepetalactone (with an average of 37.9%), while the oils in cluster 2, contained higher percentage of 1,8-cineole (52–59%). Most of the populations in cluster 2, were originated from high, cold, rainy, and steep areas.
Postoperative high-sensitivity troponin T predicts 1-year mortality and days alive and out of hospital after orthotopic heart transplantation
Authors: M’Pembele René , Roth Sebastian , Nucaro Anthony , Stroda Alexandra , Tenge Theresa , Lurati Buse Giovanna , Bönner Florian , Scheiber Daniel , Ballázs Christina , Tudorache Igor , Aubin Hug , Lichtenberg Artur , Huhn Ragnar , Boeken Udo 
Published: January 2023

Abstract
Background
Orthotopic heart transplantation (HTX) is the gold standard to treat end-stage heart failure. Numerous risk stratification tools have been developed in the past years. However, their clinical utility is limited by their poor discriminative ability. High sensitivity troponin T (hsTnT) is the most specific biomarker to detect myocardial cell injury. However, its prognostic relevance after HTX is not fully elucidated. Thus, this study evaluated the predictive value of postoperative hsTnT for 1-year survival and days alive and out of hospital (DAOH) after HTX.

Methods
This retrospective cohort study included patients who underwent HTX at the University Hospital Duesseldorf, Germany between 2011 and 2021. The main exposure was hsTnT concentration at 48 h after HTX. The primary endpoints were mortality and DAOH within 1 year after surgery. Receiver operating characteristic (ROC) curve analysis, logistic regression model and linear regression with adjustment for risk index for mortality prediction after cardiac transplantation (IMPACT) were performed.

Results
Out of 231 patients screened, 212 were included into analysis (mean age 55 ± 11 years, 73% male). One-year mortality was 19.7% (40 patients) and median DAOH was 298 days (229–322). ROC analysis revealed strongest discrimination for mortality by hsTnT at 48 h after HTX [AUC = 0.79 95% CI 0.71–0.87]. According to Youden Index, the cutoff for hsTnT at 48 h and mortality was 1640 ng/l. After adjustment for IMPACT score multivariate logistic and linear regression showed independent associations between hsTnT and mortality/DAOH with odds ratio of 8.10 [95%CI 2.99–21.89] and unstandardized regression coefficient of −1.54 [95%CI −2.02 to −1.06], respectively.

Conclusion
Postoperative hsTnT might be suitable as an early prognostic marker after HTX and is independently associated with 1-year mortality and poor DAOH.
Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella Typhimurium
Authors: Wang Weiwei , Ou Jingseng , Ye Hui , Cao Qingyun , Zhang Changming , Dong Zemin , Feng Dingyuan , Zuo Jianjun 
Published: January 2023

Abstract
Background
Salmonella Typhimurium challenge causes a huge detriment to chicken production. N-acyl homoserine lactonase (AHLase), a quorum quenching enzyme, potentially inhibits the growth and virulence of Gram-negative bacteria. However, it is unknown whether AHLase can protect chickens against S. Typhimurium challenge. This study aimed to evaluate the effects of AHLase on growth performance and intestinal health in broilers challenged by S. Typhimurium. A total of 240 one-day-old female crossbred broilers (817C) were randomly divided into 5 groups (6 replicates/group): negative control (NC), positive control (PC), and PC group supplemented with 5, 10 or 20 U/g AHLase. All birds except those in NC were challenged with S. Typhimurium from 7 to 9 days of age. All parameters related to growth and intestinal health were determined on d 10 and 14.

Results
The reductions (P < 0.05) in body weight (BW) and average daily gain (ADG) in challenged birds were alleviated by AHLase addition especially at 10 U/g. Thus, samples from NC, PC and PC plus 10 U/g AHLase group were selected for further analysis. S. Typhimurium challenge impaired (P < 0.05) intestinal morphology, elevated (P < 0.05) ileal inflammatory cytokines (IL-1β and IL-8) expression, and increased (P < 0.05) serum diamine oxidase (DAO) activity on d 10. However, AHLase addition normalized these changes. Gut microbiota analysis on d 10 showed that AHLase reversed the reductions (P < 0.05) in several beneficial bacteria (e.g. Bacilli, Bacillales and Lactobacillales), along with increases (P < 0.05) in certain harmful bacteria (e.g. Proteobacteria, Gammaproteobacteria, Enterobacteriaceae and Escherichia/Shigella) in PC group. Furthermore, AHLase-induced increased beneficial bacteria and decreased harmful bacteria were basically negatively correlated (P < 0.05) with the reductions of ileal IL-1β and IL-8 expression and serum DAO activity, but positively correlated (P < 0.05) with the increased BW and ADG. Functional prediction revealed that AHLase abolished S. Typhimurium-induced upregulations (P < 0.05) of certain pathogenicity-related pathways such as lipopolysaccharide biosynthesis, shigellosis, bacterial invasion of epithelial cells and pathogenic Escherichia coli infection of gut microbiota.

Conclusions
Supplemental AHLase attenuated S. Typhimurium-induced growth retardation and intestinal disruption in broilers, which could be associated with the observed recovery of gut microbiota dysbiosis.
Application of carrot waste extract and Lactobacillus plantarum in Alyssum homalocarpum seed gum-alginate beads to create a functional synbiotic yogurt
Authors: Sharifi Zahra , Jebelli Javan Ashkan , Hesarinejad Mohammad Ali , Parsaeimehr Mahnoosh 
Published: January 2023

AbstractOne of the most recent and effective methods, which is currently receiving special attention and is being developed by numerous researchers, is production of microspheres from the probiotic cells. The largest market segment for functional foods is represented by dairy products, which have been touted as the most effective carriers of nutrients, such as probiotics, prebiotics, proteins, vitamins, and minerals. Yogurt is fermented dairy product that is popular all over the world. A new functional symbiotic yogurt fabricated by plant wastes (carrot pomace extract), Lactobacillus plantarum, and beads based on Alyssum homolocarpum seed gum (AHSG) and sodium alginate (SA) using extrusion technique was produced and characterized. Evaluation of the functional properties of yogurts indicated that the total phenolic content and DPPH radical scavenging activity were in the range of 16.13–48.30 µg GAE/ml and 7.4–14.64%, respectively. The acidity, pH, syneresis, water holding capacity, lightness, redness, and yellowness of the yogurts were in the range of 1.50–2.90, 4.07–4.38, 49.00–57.24%, 46.8–57.3%, 57.16–61.25, − 0.20–0.91, 6.40–13.06 on the 28th day storage, respectively. The panelists confirmed the sensory properties of yogurt samples. Probiotic survival rate of the functional yogurts were in the range of 6.37–8.13 log CFU/g, on the 28th day. Based on the results, bead production by AHSG and SA and the use of carrot pomace extract enhanced the survival of probiotic bacteria significantly in yogurt during storage compared to free cells.
Graphical Abstract
Single station bias calculation using data from calibrated GNSS station for various baseline distances
Authors: Shaikh Muhammad Mubasshir 
Published: January 2023

AbstractPrecise ionospheric TEC can be derived from dual-frequency GNSS carrier phase leveled pseudorange measurements. However, differential code biases (DCB) of satellite and receiver are the main errors that cannot be ignored for precise TEC calculation. We have proposed a method of calculating station DCB using calibrated STEC data from a baseline GNSS station. The method is simply based on the understanding that the ionosphere observed by two baseline GNSS stations at the same universal time (UT) can be considered similar and would pose similar delay to the signals propagating to the two stations. The method is tested for different baseline distances of 250–1000 km and in different latitudinal regions. For 500 km baseline, the average DCB calculation error for one year data is less than 0.22 ns, 0.11 ns, and 0.25 ns for low, mid and high latitude regions, respectively. The most consistent results were obtained from high latitudes where the standard deviation remains less than 0.22 ns. The least accurate were the low latitude results where the spread of error were between 0.29 to 0.50 ns. Results showed that the accuracy and consistency of the DCB estimation reduced with the increasing baseline distance between the two participating GNSS stations. This was specifically true for low latitude regions.
The New Oncologic Diagnosis Discussion: Perspectives of Pediatric Oncologists
Authors: Six Kathryn A. , Wadhwa Aman , York Jocelyn M. , Adams Kandice , Henneberg Harrison , Bhatia Smita , Landier Wendy 
Published: January 2023

PURPOSE: We aimed to understand how new diagnosis discussions are conducted in pediatric oncology, and the training provided for their conduct. METHODS: This mixed-methods study used a sequential exploratory design. Qualitative interviews (n = 20) were conducted with pediatric oncologists (n = 15) and fellows (n = 5) at a single institution, focusing on the process used to convey the diagnosis and treatment plan to the family. Accreditation Council for Graduate Medical Education–accredited pediatric oncology fellowship program directors (n = 38) and fellows (n = 70) were subsequently surveyed to confirm qualitative results and elucidate the training that fellows receive in conducting new diagnosis discussions. RESULTS: Our findings suggest that new diagnosis discussions in pediatric oncology are typically conducted in three stages: (1) concern for cancer; (2) confirmation of diagnosis; and (3) treatment plan/consent, and are fundamentally similar across settings; however, pediatric oncologists skillfully tailor their approach on the basis of clinical circumstances and parental needs. Decisions regarding inclusion of the child are primarily determined by parental preference, whereas inclusion of health care team members is driven by physician role (ie, trainee v program director) and health care organization–related factors. Physician preparation for discussions involves logistical, intellectual, and emotional components. Disclosure of prognosis is nuanced. There is variability across pediatric oncology fellowship programs in the provision of training for these discussions. CONCLUSION: We identified common practices of pediatric oncologists as they prepare for and lead new diagnosis discussions in pediatric oncology. We found variability in the training that pediatric oncology fellows receive regarding how to conduct these discussions, highlighting a need for standardized training curricula.
Pan-cancer Landscape of Programmed Death Ligand-1 and Programmed Death Ligand-2 Structural Variations
Authors: Hoskins Emily L. , Samorodnitsky Eric , Wing Michele R. , Reeser Julie W. , Hopkins Julia F. , Murugesan Karthikeyan , Kuang Zheng , Vella Raven , Stein Leah , Risch Zachary , Yu Lianbo , Adebola Serifat , Paruchuri Anoosha , Carpten John , Chahoud Jad , Edge Stephen , Kolesar Jill , McCarter Martin , Nepple Kenneth G. , Reilley Matthew , Scaife Courtney , Tripathi Abhishek , Single Nancy , Huang Richard S.P. , Albacker Lee A. , Roychowdhury Sameek 
Published: January 2023

PURPOSE Programmed cell death protein-1 (PD-1) receptor and ligand interactions are the target of immunotherapies for more than 20 cancer types. Biomarkers that predict response to immunotherapy are microsatellite instability, tumor mutational burden, and programmed death ligand-1 (PD-L1) immunohistochemistry. Structural variations (SVs) in PD-L1 ( CD274) and PD-L2 ( PDCD1LG2) have been observed in cancer, but the comprehensive landscape is unknown. Here, we describe the genomic landscape of PD-L1 and PD-L2 SVs, their potential impact on the tumor microenvironment, and evidence that patients with these alterations can benefit from immunotherapy. METHODS We analyzed sequencing data from cancer cases with PD-L1 and PD-L2 SVs across 22 publications and four data sets, including Foundation Medicine Inc, The Cancer Genome Atlas, International Cancer Genome Consortium, and the Oncology Research Information Exchange Network. We leveraged RNA sequencing to evaluate immune signatures. We curated literature reporting clinical outcomes of patients harboring PD-L1 or PD-L2 SVs. RESULTS Using data sets encompassing 300,000 tumors, we curated 486 cases with SVs in PD-L1 and PD-L2 and observed consistent breakpoint patterns, or hotspots. Leveraging The Cancer Genome Atlas, we observed significant upregulation in PD-L1 expression and signatures for interferon signaling, macrophages, T cells, and immune cell proliferation in samples harboring PD-L1 or PD-L2 SVs. Retrospective review of 12 studies that identified patients with SVs in PD-L1 or PD-L2 revealed > 50% (52/71) response rate to PD-1 immunotherapy with durable responses. CONCLUSION Our findings show that the 3′-UTR is frequently affected, and that SVs are associated with increased expression of ligands and immune signatures. Retrospective evidence from curated studies suggests this genomic alteration could help identify candidates for PD-1/PD-L1 immunotherapy. We expect these findings will better define PD-L1 and PD-L2 SVs in cancer and lend support for prospective clinical trials to target these alterations.
Role of apoptotic inhibitors, viability, and differentiation in low oxygen tension of mesenchymal stem cells cultured in a rat model of ovarian failure
Authors: Safitri Erma , Purnobasuki Hery , Purnama Muhammad Thohawi Elziyad , Chhetri Shekhar 
Published: January 2023

Background:Stem cell therapy shows applications potential for malnutrition-induced ovarian failure in rat models. However, it is ineffective because of the lack of viability and differentiation of transplanted stem cells, resulting in low adaptation and survival rates. We aimed to determine whether stem cells cultured under low oxygen (O2) tension improves the adaptability and viability of stem cells, as well as ovarian failure.Methods: After four days of culturing mesenchymal stem cells (MSCs) in 21% oxygen (normoxia) as the T2 group and 1% oxygen (low O2or hypoxia) as the T1 group, 200 million bone marrow-derived MSCs per rat were transplanted into female rats with ovarian failure (15 rats per treatment group). A total of 15 fertile and 15 infertile rats were categorized as the C+ and C− groups, respectively.Results: The slight increase in cells expressing HSP70 (C+, T2, T1, and C− groups were 0.5a±0.53, 1.7a±0.82, 6.2b±1.5, and 9.6c±1.3, respectively), decrease in cells expressing caspase-3 as an apoptotic inhibitor (C+, T2, T1, and C− groups were 0.2a±0.42, 0.6a±0.52, 4.8b±1.03, and 7.3c±1.42, respectively), and increase in cells expressing VEGF-1 (C+, T2, T1, and C− groups were 10.8c±1.55, 8.7b±0.48, 0.4a±0.52, and 0.2a±0.42, respectively) and GDF-9 (C+, T2, T1, and C− groups were 5.8c±1.47, 4.6b±0.97, 0.5a±0.53, and 0.3a±0.48, respectively) were used as markers for viability and differentiation in ovarian tissue, indicating that MSCs cultured under low O2tension were more effective than those cultured under normoxic conditions as a treatment for female rats with ovarian failure. Furthermore, infertile female rats treated with MSCs cultivated under low O2tension had an enhanced ovarian tissue shape, as indicated by the increasing Graafian follicle count (C+, T2, T1, and C− groups were 8.9c±0.74, 4.5b±0.71, 0.5a±0.53, and 0.4a±0.52, respectively).Conclusions: MSCs cultured under low O2tension are an effective treatment for malnourished rats with ovarian failure.
Phenotypically and genotypically estimation of virulence factors in Salmonella serovar typhi isolated from patients with enteric fever in Al- Najaf, Iraq
Authors: SALIM LIQAA , MOTAWEQ ZAHRAA YOSIF 
Published: January 2023

Abstract. Zghair LS, Motaweq ZY, Lafta HC. 2022. Phenotypically and genotypically estimation of virulence factors in Salmonella serovar typhi isolated from patients with enteric fever in Al-Najaf, Iraq. Nusantara Bioscience 14: 128-133. Salmonella serovar Typhi, often known as enteric fever, causes typhoid fever and has been a major human infectious disease for centuries, surviving in poor sanitation and overcrowding. Only 64 (58.1%) Gram-negative bacteria were found from the 110 total specimens, with 46 (41.8%) Gram-positive bacteria. The 64 samples were divided into 42 (65.6%) males and 22 (34.4%) females. This work presents the isolation and identification of 64 Salmonella typhi isolates obtained from specimens. In addition, the flagellin gene was found in 64 isolated probable typhoid fever patients (fliC-d). In this study, phenotypic techniques were used to detect several virulence factors. The results showed that small colonies of L-form bacteria grow on the edges of a petri dish when one of the ?-lactam antibiotics (a class of antibiotics that includes penicillin) is given to wild-type bacteria, showing 52 (81.3%) of isolates could produce L-form and observed in 44 (68.75%). The ability to generate CFA/I and CFA/II were found in 68.7% of isolates. The large percentage of CFA produced showed CFA/III production, 64 (100%).
A DIFUSÃO DAS ESTRATÉGIAS DE ENSINO ATIVAS NO ENSINO EM CIÊNCIAS CONTÁBEIS DE UMA INSTITUIÇÃO PÚBLICA FEDERAL
Authors: LIMA THALES BATISTA DE 
Published: January 2023

O artigo busca compreender a percepção de discentes de um curso de graduação em ciências contábeis em uma instituição de ensino superior emrelação ao uso de estratégias de ensino ativas em uma disciplina de cunhoteórico-prática. Fundamenta-se pelas discussões teóricas recentes acerca das estratégias de ensino ativas no ambiente de ensino e aprendizagem. Baliza-se pelo interpretativismo, cuja abordagem metodológica é a qualitativa. O estudo ocorre em uma disciplina de um curso de ciências contábeis de uma universidade pública. Foi realizado um grupo focal com estudantes da disciplina, tendo encontrado na análise do discurso a melhor técnica para discutir os resultados. Portanto, foram identificados a satisfaçãopor distintas estratégias de ensino ativas, o impacto delas no desenvolvimento de conhecimentos e competências, além do incentivo ao comprometimento do aluno. Porém, reflete-se fatores mediadores na difusão da aplicação de estratégias de ensino ativas para promover uma formação consistente. Conclui-se que a difusão por tal uso nos cursos de ciênciascontábeis ainda apresenta algumas barreiras a serem superadas.
Consulta Araucanía: el uso oportunista de los mecanismos de democracia directa
Authors: Corvalan Alejandro , Durán Micco Elisa 
Published: January 2023

Los mecanismos de democracia directa han aumentado su presencia en todo el mundo. Junto a los beneficios que conlleva la participación ciudadana, estos mecanismos han reabierto la posibilidad de usar elecciones con propósitos movilizadores. Este artículo revisa el uso oportunista de la Consulta Araucanía, llevada a cabo en el sur de Chile el año 2021. Esta consulta ciudadana preguntó acerca de una posible extensión de la militarización en el contexto del conflicto entre el Estado chileno y el pueblo mapuche. A partir del análisis estadístico, nuestro trabajo muestra que la consulta no representó las preferencias de la región, sino que movilizó a una parte de ella en función de un objetivo particular. Sus resultados, no obstante, fueron interpretados desde la retórica de la representación con fines políticos.