bluuebunny/crossref_metadata_2025_split huggingface dataset:
When Competitors Join Forces: Consortia of Entomopathogenic Microorganisms Increase Killing Speed and Mortality in Leaf- and Root-Feeding Insect Hosts
Authors: Spescha Anna , Zwyssig Maria , Hess Hermida Mathias , Moix Aurélie , Bruno Pamela , Enkerli Jürg , Campos-Herrera Raquel , Grabenweger Giselher , Maurhofer Monika 
Published: February 2023

AbstractCombining different biocontrol agents (BCA) is an approach to increase efficacy and reliability of biological control. If several BCA are applied together, they have to be compatible and ideally work together. We studied the interaction of a previously selected BCA consortium of entomopathogenic pseudomonads (Pseudomonas chlororaphis), nematodes (Steinernema feltiae associated with Xenorhabdus bovienii), and fungi (Metarhizium brunneum). We monitored the infection course in a leaf- (Pieris brassicae) and a root-feeding (Diabrotica balteata) pest insect after simultaneous application of the three BCA as well as their interactions inside the larvae in a laboratory setting. The triple combination caused the highest mortality and increased killing speed compared to single applications against both pests. Improved efficacy against P. brassicae was mainly caused by the pseudomonad-nematode combination, whereas the nematode-fungus combination accelerated killing of D. balteata. Co-monitoring of the three BCA and the nematode-associated Xenorhabdus symbionts revealed that the four organisms are able to co-infect the same larva. However, with advancing decay of the cadaver there is increasing competition and cadaver colonization is clearly dominated by the pseudomonads, which are known for their high competitivity in the plant rhizosphere. Altogether, the combination of the three BCA increased killing efficacy against a Coleopteran and a Lepidopteran pest which indicates that this consortium could be applied successfully against a variety of insect pests.
Graphical Abstract
Controlling the physical properties of polyacrylonitrile by strontium hexaferrite nanoparticles
Authors: Shoubak Walaa M. , Hassan A. , Mahrous S. , Hassen A. 
Published: February 2023

AbstractThe formulation of polymer with embedded magnetic nanoparticles results in promising nanocomposites for smart and analytical applications. Nanocomposites containing polyacrylonitrile (PAN) and different mass contents of strontium hexaferrite (SFO) were prepared using the casting method. The nanocomposite samples were characterized by using different techniques such as field-emission scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, differential scanning calorimetry, and thermogravimetric analysis. Dielectric investigations of SFO/PAN nanocomposites showed that the permittivity and conductivity are considerably enhanced as the content of SFO increased. Optical properties revealed that the absorption and transmittance spectra were significantly affected by adding SFO nanoparticles to the PNA polymer matrix. To investigate the magnetic properties of the nanocomposite samples, the vibrating sample magnetometer was used. The magnetic hysteresis loops illustrated the ferromagnetic nature of SFO/PAN nanocomposites. Different magnetic parameters were given, and they depend on the content of PAN in the nanocomposites.
Occupational asbestos exposure and urinary bladder cancer: a systematic review and meta-analysis
Authors: Franco Nicolò , Godono Alessandro , Clari Marco , Ciocan Catalina , Zunarelli Carlotta , Pira Enrico , Boffetta Paolo 
Published: February 2023

Abstract
Purpose
There is conflicting evidence on the association between asbestos exposure and bladder cancer. We performed a systematic review and meta-analysis to provide evidence on occupational asbestos exposure and the risk of mortality and incidence of bladder cancer.

Methods
We searched three relevant electronic databases (Pubmed, Scopus, and Embase) from inception to October 2021. The methodological quality of included articles was evaluated using the US National Institutes of Health tool. Standardized incidence ratios (SIRs) and standardized mortality ratios (SMRs) for bladder cancer, as well as respective 95% confidence intervals (CIs), were extracted or calculated for each included cohort. Main and subgroup meta-analyses according to first year of employment, industry, sex, asbestos type, and geographic region were performed.

Results
Fifty-nine publications comprising 60 cohorts were included. Bladder cancer incidence and mortality were not significantly associated with occupational asbestos exposure (pooled SIR: 1.04, 95% CI: 0.95–1.13, P = 0.000; pooled SMR: 1.06, 95% CI: 0.96–1.17, P = 0.031). Bladder cancer incidence was higher among workers employed between 1908 and 1940 (SIR: 1.15, 95% CI: 1.01–1.31). Mortality was elevated in asbestos workers cohorts (SMR: 1.12, 95% CI: 1.06–1.30) and in the subgroup analysis for women (SMR: 1.83, 95% CI: 1.22–2.75). No association was found between asbestos types and bladder cancer incidence or mortality. We observed no difference in the subgroup analysis for countries and no direct publication bias evidence.

Conclusion
There is evidence that workers with occupational asbestos exposure have a bladder cancer incidence and mortality similar to the general population.
New SNCA mutation and structures of α-synuclein filaments from juvenile-onset synucleinopathy
Authors: Yang Yang , Garringer Holly J. , Shi Yang , Lövestam Sofia , Peak-Chew Sew , Zhang Xianjun , Kotecha Abhay , Bacioglu Mehtap , Koto Atsuo , Takao Masaki , Spillantini Maria Grazia , Ghetti Bernardino , Vidal Ruben , Murzin Alexey G. , Scheres Sjors H. W. , Goedert Michel 
Published: February 2023

AbstractA 21-nucleotide duplication in one allele of SNCA was identified in a previously described disease with abundant α-synuclein inclusions that we now call juvenile-onset synucleinopathy (JOS). This mutation translates into the insertion of MAAAEKT after residue 22 of α-synuclein, resulting in a protein of 147 amino acids. Both wild-type and mutant proteins were present in sarkosyl-insoluble material that was extracted from frontal cortex of the individual with JOS and examined by electron cryo-microscopy. The structures of JOS filaments, comprising either a single protofilament, or a pair of protofilaments, revealed a new α-synuclein fold that differs from the folds of Lewy body diseases and multiple system atrophy (MSA). The JOS fold consists of a compact core, the sequence of which (residues 36–100 of wild-type α-synuclein) is unaffected by the mutation, and two disconnected density islands (A and B) of mixed sequences. There is a non-proteinaceous cofactor bound between the core and island A. The JOS fold resembles the common substructure of MSA Type I and Type II dimeric filaments, with its core segment approximating the C-terminal body of MSA protofilaments B and its islands mimicking the N-terminal arm of MSA protofilaments A. The partial similarity of JOS and MSA folds extends to the locations of their cofactor-binding sites. In vitro assembly of recombinant wild-type α-synuclein, its insertion mutant and their mixture yielded structures that were distinct from those of JOS filaments. Our findings provide insight into a possible mechanism of JOS fibrillation in which mutant α-synuclein of 147 amino acids forms a nucleus with the JOS fold, around which wild-type and mutant proteins assemble during elongation.
Coronal extrusion of the lateral meniscus does not increase after pullout repair of the posterior root of the lateral meniscus at short-term follow-up
Authors: Forkel Philipp , Noack Jonas , Hinz Maximilian , Imhoff Andreas B. , Wörtler Klaus , Feucht Matthias J. 
Published: February 2023

Abstract
Purpose
Posterior lateral meniscus root (PLMR) tears are injuries that commonly occur together with anterior cruciate ligament (ACL) tears. The aim of this study was to evaluate the clinical and radiological outcome of PLMR repair accompanying ACL reconstruction. Specifically, PLMR healing rates, meniscal extrusion behavior and their influence on patient-reported outcome measures (PROMs) were analyzed. It was hypothesized that PLMR repair shows satisfactory healing rates and coronal meniscal extrusion does not increase significantly following PLMR repair.

Methods
Patients that underwent PLMR repair between 2014 and 2019 were evaluated at least 12 months postoperatively. At follow-up, magnetic resonance imaging (MRI) was performed in order to evaluate the PLMR healing behavior (complete vs. partial vs. no healing) as well as the coronal and sagittal meniscal extrusion in comparison with the preoperative MRI. Additionally, patient-reported outcome measures (PROMs; Lysholm score, International Knee Documentation Committee subjective knee form [IKDC]) were compiled. Pre- and postoperative meniscal extrusion were tested for statistical significance using the paired t test. The Kruskal–Wallis test was used to compare extrusion values and PROMs in relation to different healing states. A correlation analysis was conducted using the Pearson correlation coefficient between differences in meniscal extrusion and PROMs.

Results
Out of 25 patients, 18 patients (72.0%; 11 male and seven female) were available for final assessment at a mean follow-up of 40.8 ± SD 17.5 months. One revision PLMR repair was performed five months after the initial repair. In 14 cases (77.8%), healing of the lateral meniscus was observed (6 × complete, 8 × partial). Coronal extrusion of the lateral meniscus did not increase significantly following PLMR repair (2.0 ± 1.5 mm vs. 2.1 ± 1.3 mm; p = 0.645). Sagittal extrusion increased significantly (25.7 ± 2.4 mm vs. 27.0 ± 1.4 mm; p < 0.001). The healing status of the PLMR showed no significant association with meniscal extrusion or PROMs (p > 0.05). But a higher increase in coronal meniscal extrusion negatively affected PROMs (Lysholm score: p = 0.046, r = − 0.475; IKDC: p = 0.003, r = − 0.651).

Conclusion
High healing rates of the PLMR and no significant increase in coronal extrusion may be expected following combined PLMR repair and ACL reconstruction. But a greater increase in postoperative coronal meniscal extrusion correlates with less favorable clinical results. A greater increase in sagittal extrusion was observed, but this did not influence the clinical outcome.

Level of evidence
Retrospective Case Series; IV.
An exploratory study of imaging diagnostic clues for overhanging facial nerve in ultra-high-resolution CT
Authors: Zhang Zhengyu , Tang Ruowei , Wu Qian , Zhao Pengfei , Yang Zhenghan , Wang Zhenchang 
Published: February 2023

Abstract
Purpose
Overhanging facial nerve (FN) may be challenging in imaging diagnosis. The purpose of the study is to investigate the imaging clues for overhanging FN near the oval window on ultra-high-resolution computed tomography (U-HRCT) images.

Methods
Between October 2020 and August 2021, images of 325 ears (276 patients) were included in the analysis obtained by an experimental U-HRCT scanner. On standard reformatted images, the morphology of FN was evaluated and its position was quantitatively measured using the following indices: protrusion ratio (PR), protruding angle (A), position of FN (P-FN), distance between FN and stapes (D-S), and distance between FN and anterior and posterior crura of stapes (D-AC and D-PC). According to the FN morphology in imaging, images were divided into overhanging FN group and non-overhanging FN group. Binary univariate logistic regression analysis was used to identify the imaging indices independently associated with overhanging FN.

Results
Overhanging FN was found in 66 ears (20.3%), which manifested as downwards protrusion of either local segment (61 ears, 61/66) or the entire course near the oval window (5 ears, 5/66). D-AC [odds ratio: 0.063, 95% CI 0.012–0.334, P = 0.001) and D-PC (odds ratio: 0.008, 95% CI 0.001–0.050, P = 0.000) were identified as independent predictors of FN overhang (area under the curve: 0.828 and 0.865, respectively).

Conclusion
Abnormal morphology of the lower margin of FN, D-AC and D-PC on U-HRCT
images provide valuable diagnostic clues for FN overhang.
The Berlin-Buch respiration chamber for energy expenditure measurements
Authors: Mähler Anja , Schütte Till , Steiniger Jochen , Boschmann Michael 
Published: February 2023

Abstract
Purpose
We present a methodological overview of a respiration chamber at the Experimental and Clinical Research Center in Berlin, Germany. Since 2010, we investigated 750 healthy subjects and patients with various diseases. We routinely measure resting energy expenditure (REE), dietary-induced thermogenesis, and activity energy expenditure.

Methods
The chamber is a pull calorimeter with a total volume of 11,000 L. The majority of measurements is done with a flow rate of 120 L/min, yielding a favorable time constant of 1.53 h. The gas analysis system consists of two paramagnetic O2 sensors and two infrared CO2 sensors, one for incoming and one for outgoing air samples. O2 and CO2 sensors are calibrated simultaneously before each measurement with a 6 min calibration routine. To verify the accuracy of the whole the calorimetric system, it is validated every 2 weeks by 2 h acetone burning tests.

Results
Validation factors (calculated/measured) of 20 representative 2 h acetone burning tests were 1.03 ± 0.03 for $$\dot{V}{\text{O}}_{2}$$

V
˙

O
2

, 1.02 ± 0.02 for $$\dot{V}{\text{CO}}_{{2}}$$

V
˙

CO
2

, 0.99 ± 0.02 for RER, and 1.03 ± 0.03 for EE. Four repeated 60 min REE measurements of a healthy woman showed variabilities of 231.9 ± 4.8 ml/min for $$\dot{V}{\text{O}}_{2}$$

V
˙

O
2

(CV 2.1%), 166.0 ± 6.3 ml/min for $$\dot{V}{\text{CO}}_{{2}}$$

V
˙

CO
2

(CV 3.8%), 0.73 ± 0.03 for RER (CV 4.6%), and 4.55 ± 0.07 kJ/min for EE (CV 1.6%).

Conclusions
The data presented show that our respiration chamber produces precise and valid EE measurements with an exceptionally fast responsiveness.

Graphical Abstract
Inhibition of ventral tegmental area projections to the nucleus accumbens shell increases premature responding in the five-choice serial reaction time task in rats
Authors: Flores-Dourojeanni Jacques P. , van den Munkhof Marleen H. , Luijendijk Mieneke C. M. , Vanderschuren Louk J. M. J. , Adan Roger A. H. 
Published: February 2023

AbstractExaggerated impulsivity and attentional impairments are hallmarks of certain disorders of behavioural control such as attention-deficit/hyperactivity disorder (ADHD), schizophrenia and addiction. Pharmacological studies have implicated elevated dopamine (DA) levels in the nucleus accumbens shell (NAcbS) in impulsive actions. The NAcbS receives its DA input from the ventral tegmental area (VTA), and we have previously shown that optogenetic activation of VTA-NAcbS projections impaired impulse control and attention in the five-choice serial reaction time task (5-CSRTT) in rats. To better understand the role of VTA-NAcbS projections in impulsivity and attention, the present study sought to inhibit this projection using optogenetics. We demonstrate that inhibiting VTA-NAcbS efferents during the last seconds of the inter-trial interval (i.e. immediately before presentation of the instructive cue) induces exaggerated impulsive action, in the absence of changes in attentional or motivational parameters in the 5-CSRTT. Together with our earlier observations, this suggests that impulse control in the 5-CSRTT is tightly controlled by VTA-NAcbS activity, with deviations in both directions resulting in increased impulsivity.
Mapping Tumor Heterogeneity via Local Entropy Assessment: Making Biomarkers Visible
Authors: Costa Guido , Cavinato Lara , Fiz Francesco , Sollini Martina , Chiti Arturo , Torzilli Guido , Ieva Francesca , Viganò Luca 
Published: February 2023

AbstractAdvanced imaging and analysis improve prediction of pathology data and outcomes in several tumors, with entropy-based measures being among the most promising biomarkers. However, entropy is often perceived as statistical data lacking clinical significance. We aimed to generate a voxel-by-voxel visual map of local tumor entropy, thus allowing to (1) make entropy explainable and accessible to clinicians; (2) disclose and quantitively characterize any intra-tumoral entropy heterogeneity; (3) evaluate associations between entropy and pathology data. We analyzed the portal phase of preoperative CT of 20 patients undergoing liver surgery for colorectal metastases. A three-dimensional core kernel (5 × 5 × 5 voxels) was created and used to compute the local entropy value for each voxel of the tumor. The map was encoded with a color palette. We performed two analyses: (a) qualitative assessment of tumors’ detectability and pattern of entropy distribution; (b) quantitative analysis of the entropy values distribution. The latter data were compared with standard Hounsfield data as predictors of post-chemotherapy tumor regression grade (TRG). Entropy maps were successfully built for all tumors. Metastases were qualitatively hyper-entropic compared to surrounding parenchyma. In four cases hyper-entropic areas exceeded the tumor margin visible at CT. We identified four “entropic” patterns: homogeneous, inhomogeneous, peripheral rim, and mixed. At quantitative analysis, entropy-derived data (percentiles/mean/median/root mean square) predicted TRG (p < 0.05) better than Hounsfield-derived ones (p = n.s.). We present a standardized imaging technique to visualize tumor heterogeneity built on a voxel-by-voxel entropy assessment. The association of local entropy with pathology data supports its role as a biomarker.
Graphical Abstract
A Phenomenological Actus Essendi? Hedwig Conrad-Martius and Edith Stein on Finite Existence
Authors: Neumann Daniel 
Published: February 2023

AbstractIn later Edith Stein and Hedwig Conrad-Martius, finite existence appears to be necessarily intertwined with infinite being. In response to this observation, this paper puts particular focus on the experience of finite being in order to address the specifically phenomenological (i.e., experiential) aspects of Stein’s and Conrad-Martius’ metaphysics. As a consequence, instead of pointing to eternal or infinite being, finite experience is understood to – less specifically – transcend itself. Using the notion ofactus essendi(priority of existence over essence), I identify two ideas as specifically characterizing this transcendence: non-ownership of time (in Conrad-Martius), by which is questioned the coherence of inner time consciousness, as well as non-ownership of sense (in Stein), stipulating that the sense one intuitively and intellectively experiences in reference to objects is discovered, rather than made. Subsequently, the paper discusses how Stein’s and Conrad-Martius’ metaphysics of finite existence is reflected in their critical assessments of Heidegger’s existential finitude.
Challenges of participatory design in apartment buildings’ renovation projects in Finland
Authors: Soikkeli Anu , Santamäki Emmi , Hynninen Oona , Äijälä Saara 
Published: February 2023

AbstractParticipatory and user-centered design has taken inspiration from each other, and the classical roles of users, researchers, and designers have merged in the co-designing process. Participatory methods consider the balance of power between the designer and residents and the needs and usefulness of the research. Community members should be involved as partners, advisors, and participants.In Finland, participatory design is law mandated in urban planning projects, but participation that goes beyond the initial publicization of information, is genuinely interactive, and spans all stages of the project is underutilised and the methods of participatory practice are still unfamiliar to many designers. The methods commonly used are the research survey and the workshop, often mentioned as participatory design methods. From the residents’ perspective, participation only becomes participatory when the participant gains the experience of influencing the development and its outcomes. The article examines resident surveys and workshops as tools to produce information and evaluate their interactivity in two case studies. It shows that the diversification of methods is necessary to ensure genuine participatory planning in the renewal of suburban neighborhoods.The research was done as part of the project Reshaping living: New structure and new life for old suburbs (AsuMut), funded by the Ministry of Environment.
How do Employees with Chronic Musculoskeletal Disorders Experience the Management of Their Condition in the Workplace? A Metasynthesis
Authors: Skamagki Glykeria , Carpenter Christine , King Andrew , Wåhlin Charlotte 
Published: February 2023

AbstractThis metasynthesis contributes to an understanding of the experiences, perceptions, and attitudes of employees on managing chronic musculoskeletal disorders (CMSDs) at work. Many studies in this field are concerned with prevention or return-to-work (RTW) programmes. However, the purpose of this review was to synthesise evidence that only focuses on the employees’ management of their CMSDs at work. The SPIDER framework was used to structure the question “How do employees with CMSDs experience the management of their condition in the workplace”? The literature search focused on articles published between 2011 and 2021, and the search was conducted using the following databases: MEDLINE, SCOPUS, CINAHL, AMED, PsycINFO. The review identified nine articles that explored employees’ experiences of managing CMSDs at work. Thematic synthesis was used to create analytic themes which provided a more in-depth discussion of these experiences. The identified themes were: ‘employees actively seek ways to manage their conditions’, ‘influence of work environment on employees with CMSDs’ and ‘optimising the relationship between employees and managers. This metasynthesis suggests that the ability to negotiate workplace support and manage CMSDs at work is influenced by the cultural and social environment of the organisation. Effective communication, care and trust between the employee is needed. The review also illustrated the need for healthcare professionals to provide support to employees at work.
Evaluation of the therapeutic potential of cerebrolysin and/or lithium in the male Wistar rat model of Parkinson’s disease induced by reserpine
Authors: Tharwat Engy K. , Abdelaty Ahmed O. , Abdelrahman Alaa I. , Elsaeed Hebatallah , Elgohary Ayatallah , El-Feky Amena S. , Ebrahim Yasmina M. , Sakraan Alaa , Ismail Hossam A. , Khadrawy Yasser A. , Aboul Ezz Heba S. , Noor Neveen A. , Fahmy Heba M. , Mohammed Haitham S. , Mohammed Faten F. , Radwan Nasr M. , Ahmed Nawal A. 
Published: February 2023

AbstractParkinson’s disease (PD) is the second most prevalent neurodegenerative disease worldwide and represents a challenge for clinicians. The present study aims to investigate the effects of cerebrolysin and/or lithium on the behavioral, neurochemical and histopathological alterations induced by reserpine as a model of PD. The rats were divided into control and reserpine-induced PD model groups. The model animals were further divided into four subgroups: rat PD model, rat PD model treated with cerebrolysin, rat PD model treated with lithium and rat PD model treated with a combination of cerebrolysin and lithium. Treatment with cerebrolysin and/or lithium ameliorated most of the alterations in oxidative stress parameters, acetylcholinesterase and monoamines in the striatum and midbrain of reserpine-induced PD model. It also ameliorated the changes in nuclear factor-kappa and improved the histopathological picture induced by reserpine. It could be suggested that cerebrolysin and/or lithium showed promising therapeutic potential against the variations induced in the reserpine model of PD. However, the ameliorating effects of lithium on the neurochemical, histopathological and behavioral alterations induced by reserpine were more prominent than those of cerebrolysin alone or combined with lithium. It can be concluded that the antioxidant and anti-inflammatory effects of both drugs played a significant role in their therapeutic potency.