bluuebunny/crossref_metadata_2025_split huggingface dataset:
Macroscopic modeling of the growth and substrate consumption of wild type and genetically modified Pichia pastoris
Authors: Joseph Jewel Ann , Akkermans Simen , Van Impe Jan F. 
Published: September 2023

AbstractPichia pastoris is a popular yeast platform to generate several industrially relevant products which have applications in a wide range of sectors. The complexities in the processes due to the addition of a foreign gene are not widely explored. Since these complexities can be dependent on the strain characteristics, promoter, and type of protein produced, it is vital to investigate the growth and substrate consumption patterns of the host to facilitate customized process optimization. In this study, the growth rates of P. pastoris GS115 wild type (WT) and genetically modified (GM) strains grown on glycerol and methanol in batch cultivation mode were estimated and the model providing the best representation of the true growth kinetics based on substrate consumption was identified. It was observed that the growth of P. pastoris exhibits Haldane kinetics on glycerol rather than the most commonly used Monod kinetics due to the inability of the latter to describe growth inhibition at high concentrations of glycerol. Whereas, the cardinal parameter model, a newly proposed model for this application, was found to be the best fitting to describe the growth of P. pastoris on methanol due to its ability to describe methanol toxicity. Interestingly, the findings from this study concluded that in both substrates, the genetically engineered strain exhibited a higher growth rate compared to the WT strain. Such an observation has not been established yet in other published works, indicating an opportunity to further optimize the carbon source feeding strategies when the host is grown in fed‐batch mode.
Two‐way reaction of versatile peroxidase with artificial lignin enhances low‐molecular weight fractions
Authors: Spasojević Dragica , Prodanović Olivera , Mutavdžić Dragosav , Sekuljica Nataša , Jovanović Jelena , Maksimović Vuk , Radotić Ksenija 
Published: September 2023

AbstractIn recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP‐ high performance liquid chromatography with mass detection (HPLC‐MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.
Recent advances in upstream process development for production of recombinant adeno‐associated virus
Authors: Ou Jianfa , Tang Yawen , Xu Jianlin , Tucci Julian , Borys Michael C. , Khetan Anurag 
Published: September 2023

AbstractRecombinant adeno‐associated virus (rAAV) is rapidly emerging as the preferred delivery vehicle for gene therapies, with promising advantages in safety and efficacy. Key challenges in systemic in‐vivo rAAV gene therapy applications are the gap in production capabilities versus potential market demand and complex production process. This review summarizes current available information on rAAV upstream manufacturing processes and proposed optimizations for production. The advancements in rAAV production media were reviewed with proposals to speed up the cell culture process development. Furthermore, major methods for genetic element delivery to host cells were summarized with their advantages, limitations, and future directions for optimization. In addition, culture vessel selection criteria were listed based on production cell system, scale, and development stage. Process control at the production step was also outlined with an in‐depth understanding of production kinetics and quality control.
Student therapists' experiences of learning using a machine client: A proof‐of‐concept exploration of an emotionally responsive interactive client (ERIC)
Authors: Prescott Julie , Ogilvie Lisa , Hanley Terry 
Published: September 2023

AbstractBackgroundThe use of artificial intelligence (AI) is increasing in many areas of healthcare, including mental healthcare. The automated nature of such technologies has the potential to be developed to work with large numbers of people. This paper examines the way that student therapists experience using an interactive text‐based machine client as a training tool.MethodologyChatbot technology has been used to develop an emotionally responsive interactive client (ERIC). This introduces individuals to concepts of person‐centred therapy (empathy, congruence and unconditional positive regard) by using a series of pre‐programmed scenarios. Twenty‐eight student therapists evaluated ERIC's potential as a learning tool. Individuals were recruited from one university from a postgraduate and an undergraduate counselling programme.FindingsFeedback was generally positive, with all reporting that they enjoyed engaging with ERIC as a learning method. ERIC helped individuals consider their understanding of counselling skills in a non‐judgemental environment. Participants felt the scenarios were realistic and engaging, with many reporting that they felt they were engaging with a real client/person due to ERIC's ability to express emotions.DiscussionERIC is at the proof‐of‐concept phase. From the feedback presented here, it is evident that it can be a useful learning tool. Further development of ERIC with feedback from a larger sample is, however, required. ERIC is currently a text‐based client, and further development would like to see the intervention be voice‐activated to enhance the experience. ERIC can be further enhanced and adapted to be a useful learning platform for student therapists, as well as for students in other (healthcare‐related) disciplines, whereby a client or patient is required.
From the iron cage to ‘La Jaula de Oro’: Music as a cultural object
Authors: Rodriguez Liliana V. 
Published: September 2023

AbstractThenorteñobandLos Tigres del Norte(LTDN) are widely known as the ‘voice’ of the undocumented community for using their music as a vehicle to create and raise social consciousness on pressing issues of immigration. Utilizing Griswold's cultural diamond approach to analysing culture, I examine the culture/society relationship that emerges from their musical repertoire. Using this sociological model of culture, I argue that the wide receptiveness of their music is based on the constructed phenomenon that more than just a celebration of dance rhythms and entertainment their music poses itself as a cultural object attached with ideologies. By appropriating the dominant national discourse that has traditionally belittled, discriminated and dehumanized immigrants in the United States, in particular immigrants of Latin American descent, the music of LTDN markets itself less as a product and more as a cultural component of identity empowering marginalized communities by not only telling their stories but also nurturing a sense of pride. Analysing 56 songs within the musical repertoire of LTDN from 1970 to 2022, I demonstrate how their music on immigrant‐related struggles serves to reinforce a Latina/o collective identity by fostering solidarity, educating the public and instilling pride.
ASER: Adapted squared error relevance for rare cases prediction in imbalanced regression
Authors: Kou Ying , Fu Guang‐Hui 
Published: September 2023

AbstractMany real‐world data mining applications involve using imbalanced datasets to obtain predictive models. Imbalanced data can hinder the model performance of learning algorithms in rare cases. Although there are many well‐researched classification task solutions, most of them cannot be directly applied to regression task. One of the challenges in imbalanced regression is to find a suitable evaluation and optimization standard that can improve the predictive ability of the model without severe model bias. Based on the importance of rare cases, this study proposes a new evaluation metric called adapted squared error relevance (ASER) by defining new relevance function and weighting functions. This metric weights data points by defining the importance of rare cases and assigns different weights to losses of the same size at different rare cases, thus enabling the model selected by this evaluation metric to better predict rare cases. ASER is compared with SER on 32 real datasets and 9 simulated datasets to verify the predictive performance of the selected model at rare cases. The experimental results show that the new evaluation metric ASER can obtain a high prediction performance at rare cases, while also not losing too much prediction accuracy in common cases.
Breast cancer survivorship experiences among Black women
Authors: Ko Naomi Y. , Fikre Tsion G. , Buck Anne K. , Restrepo Emily , Warner Erica T. 
Published: September 2023

AbstractBackgroundBlack women experience significant disparities in breast cancer across the care continuum, including survivorship. Ensuring that Black women obtain high‐quality follow‐up care is critical but understudied. This study was aimed at understanding the experiences and needs of Black women during breast cancer survivorship.MethodsBlack patients diagnosed with invasive breast cancer within the past 5 years were invited to participate in a focus group and complete a survey. Focus groups examined the following: (1) the transition from active treatment to survivorship; (2) interactions with health care providers; (3) survivorship experiences, information needs, and preferences; and (4) existing educational materials. Results were thematically coded and analyzed for main themes. Surveys collected information on sociodemographics, health care experiences, quality of life, lifestyle, and education needs.ResultsThe study enrolled 53 participants, 43 of whom completed a survey and participated in one of 11 focus groups. The median age was 54 years, 44% had private insurance, 81% were English speaking, and 86% had completed their treatment more than a year before. Participants identified the importance of relationships with health care providers, gaps in survivorship care, experiences with cancer‐related symptoms, challenges with mental health, worry about recurrence, body image, cancer financial toxicity, and coping through religion and spirituality. Unmet needs were centered around preparation for long‐term symptoms, diet and physical activity, emotional support, and more explanations of information resources. Participants reported preferences for educational videos, personal stories, and culturally relevant content.ConclusionsSome Black breast cancer survivors may have specific challenges and preferences. Supportive interventions that address these concerns can be responsive and help to ameliorate disparities.
Mammography screening beliefs and barriers through the lens of Black women during the COVID‐19 pandemic
Authors: Bea Vivian J. , An Anjile , Gordon Ashley M. , Antoine Francesse S. , Wiggins Plyshette Y. , Hyman Diane , Robles‐Rodriguez Evelyn 
Published: September 2023

AbstractBackgroundMammography is an effective screening tool that leads to decreased breast cancer mortality, yet minority women continue to experience barriers. The coronavirus disease 2019 (COVID‐19) pandemic has been proven to have negatively affected minority communities, yet its effect on mammography screening habits in Black women is uncertain. The purpose of this study was to evaluate breast cancer mammography screening habits and barriers for Black women in two northeast communities amid the COVID‐19 pandemic.MethodsThe study participants were Black women aged 40 years or older who were recruited from community outreach initiatives. Study coordinators conducted telephone surveys to determine mammography screening behaviors, perceptions, and psychosocial factors.ResultsTwo hundred seventy‐seven surveys were completed. Two hundred fifty‐six patients who reported ever having a mammogram became the study population of interest. One hundred seventy‐four of these patients (68%) reported having a mammogram within the past year (nondelayed), and 82 (32%) had a mammogram more than a year ago (delayed). Only thirty‐one of the delayed participants (37.8%) had private insurance. There was a significant difference in the mean score for mammography screening perceived barriers for nondelayed participants (mean = 9.9, standard deviation [SD] = 3.6) versus delayed participants (mean = 11.2, SD = 4.3, p = .03). There was also a significant difference in the mean score when they were asked, “How likely is it that ‘other health problems would keep you from having a mammogram’?” (p = .002).ConclusionsBarriers to mammography screening for Black women during the COVID‐19 era include insurance, competing health issues, and perceptions of screening. Community outreach efforts should concentrate on building trust and collaborating with organizations to improve screening despite the COVID‐19 pandemic.
NiO/ZnO Composite Derived Metal‐Organic Framework as Advanced Electrode Materials for Zinc Hybrid Redox Flow Battery
Authors: Lakshmanan Priya , Janshirani Premnahth Jeyaraj , Rengapillai Subadevi , Sivakumar Marimuthu 
Published: September 2023

AbstractNiO/ZnO composite derived metal‐organic framework (MOF) is used as to modify carbon felt (CF) via a conventional solid‐state reaction followed by ultrasonication. The prepared electrode material is used in zinc‐hybrid redox flow batteries (RFBs) due to their high redox activity of Zn2+/Zn. The electrochemical performance of composite modified CF and pre‐treated CF was studied by cyclic voltammetry (CV) in 0.5 M aqueous zinc chloride with 5 M potassium hydroxide solutions showed clear confirmation for enhanced electrocatalytic activity. The unique porous structure of NiO/ZnO‐derived MOF with increased surface area improves the battery behavior significantlyThe peak current ratio for the as‐prepared material is about 3 times higher than that of the pre‐treated CF due to more active sites. Zinc‐based RFB with modified CF electrode exhibited better electrochemical performance with voltage efficiency (VE, 88 %), which is higher than true redox flow batteries.
A wide input range, 94% peak efficiency auto‐select multi‐mode boost converter for thermoelectric energy harvesting
Authors: Hajiakbari Fatemeh , Ashraf Mohammadreza 
Published: September 2023

SummaryIn this paper, a high‐efficiency DC–DC boost converter is proposed for thermal energy harvesting systems. The converter's efficiency is improved using a low‐power digital operating mode detector circuit. The operating mode detector circuit changes the converter's operating mode between the continuous conduction mode (CCM) and discontinuous conduction mode (DCM) at a specific input voltage (boundary input voltage), which reduces the total losses, thus improves the efficiency and increases the input voltage range. Besides, the proper boundary input voltage is calculated as a function of the boost converter's parameters. Finally, the proposed converter is designed in a standard 0.18 μm CMOS process. Simulation results show that the peak efficiency of the converter can be up to 94%, 60.4%, and 35% at 300, 20, and 15 mV of the open‐circuit voltage, respectively.
Hypothyroidism and subclinical hypothyroidism are associated with fatty pancreas (Non‐Alcoholic Fatty Pancreas Disease)
Authors: Bayyigit Akif , Gokden Yasemin , Onol Suzan , Ozek Fatma Z. , Saglam Selin , Adas Mine 
Published: September 2023

AbstractObjectivesIncreasing visceral fat deposition with raised prevalence of obesity and metabolic syndrome is associated with many adverse conditions, especially cardiovascular diseases and diabetes. Although there are many studies that investigate hepatic steatosis in hypothyroidism and subclinical hypothyroidism, to the best of our knowledge, there is no study investigating its relationship with pancreatic steatosis. In the present study, the purpose was to investigate this relationship.MethodsPhysical and biochemical characteristics of 30 hypothyroid, 30 subclinical hypothyroid, and 30 euthyroid volunteers were recorded in this cross‐sectional study. Liver and pancreatic steatosis were evaluated with ultrasonography.ResultsIt was found that pancreatic steatosis was increased in hypothyroid and subclinical groups when compared to the control group, and hepatic steatosis was increased in the subclinical group when compared to the control group (steatosis; p = 0.002, p = 0.004, p = 0.001, p = 0.002, p = 0.002, p = 0.004). Pancreatic steatosis was positively correlated with age, hepatic steatosis, height, weight, BMI, waist circumference, hip circumference, hemoglobin, Insulin, alanine aminotransferase, Triglyceride, Creatinine, and gamma‐glutamyltransferase and was negatively correlated with total cholesterol, high‐density lipoproteins.ConclusionsThe prevalence of pancreatic steatosis was found to be increased in hypothyroidism and subclinical hypothyroidism when compared with the euthyroid control group.
Detection of capromorelin in urine following oral and dermal routes of administration
Authors: Sobolevsky Tim , Walpurgis Katja , Goergens Christian , Fedoruk Matthew , Lewis Laura , Ahrens Brian , Thevis Mario 
Published: September 2023

AbstractCapromorelin is a growth hormone secretagogue. Despite promising results to alleviate muscle‐wasting in the elderly, it has not advanced further in human development. Subsequent studies demonstrated capromorelin's ability to increase food intake in animals, leading to approval in the United States and Europe as an appetite stimulant for cats (Elura) and dogs (Entyce). Capromorelin is prohibited in sports due to its ability to stimulate growth hormone production and enhance performance. However, given that its veterinary preparation is formulated as a highly concentrated solution (20 or 30 mg/mL) delivered orally, incidental ingestion or dermal absorption may result in an adverse analytical finding (AAF) by way of direct exposure during oral administration to a pet. An administration study was conducted by either oral or transdermal application of capromorelin solution to mimic the scenario of inadvertent exposure to the drug. Ingestion of 30 μg of capromorelin orally (equivalent to 1 μL of Entyce) resulted in detectable amounts of capromorelin in urine for up to 48 h after administration with a maximum urinary concentration of 7 ng/mL. Importantly, when applied directly to the skin on the hands in larger quantities mimicking a pet administration exposure scenario (30 mg or 1 mL of Entyce), capromorelin was also detected reaching a maximum urinary concentration of 0.7 ng/mL. Athletes and testing authorities should be aware of the risk of an AAF arising due to incidental exposure to veterinary preparations of capromorelin. To our knowledge, before 2022, no positive test for capromorelin had ever been reported.
Distinct Heterocyclic Moieties Govern the Selectivity of Thiophene‐Vinylene‐Based Ligands towards Aβ or Tau Pathology in Alzheimer's Disease
Authors: Björk Linnea , Shirani Hamid , Todarwal Yogesh , Linares Mathieu , Vidal Ruben , Ghetti Bernardino , Norman Patrick , Klingstedt Therése , Nilsson K. Peter R. 
Published: September 2023

AbstractDistinct aggregated proteins are correlated with numerous neurodegenerative diseases and the development of ligands that selectively detect these pathological hallmarks is vital. Recently, the synthesis of thiophene‐based optical ligands, denoted bi‐thiophene‐vinyl‐benzothiazoles (bTVBTs), that could be utilized for selective assignment of tau pathology in brain tissue with Alzheimer's disease (AD) pathology, was reported. Herein, we investigate the ability of these ligands to selectively distinguish tau deposits from aggregated amyloid‐β (Aβ), the second AD associated pathological hallmark, when replacing the terminal thiophene moiety with other heterocyclic motifs. The selectivity for tau pathology was reduced when introducing specific heterocyclic motifs, verifying that specific molecular interactions between the ligands and the aggregates are necessary for selective detection of tau deposits. In addition, ligands having certain heterocyclic moieties attached to the central thiophene‐vinylene building block displayed selectivity to aggregated Aβ pathology. Our findings provide chemical insights for the development of ligands that can distinguish between aggregated proteinaceous species consisting of different proteins and might also aid in creating novel agents for clinical imaging of tau pathology in AD.
Diaminocyclopentadienone Ruthenium Complex Catalyzed Alkylation of Indoles and Ketones with Primary Alcohols
Authors: Skowaisa Steffen , Haak Edgar 
Published: September 2023

AbstractA diaminocyclopentadienone ruthenium complex has proven to be a highly effective catalyst for the alkylation of indoles or ketones with poorly reactive alcohols. The catalyst is readily available, air and moisture stable and exhibits wide functional group tolerance. The environmentally benign procedure follows a borrowing hydrogen mechanism and requires no excess of either component. Various indoles and ketones are selectively monoalkylated in high yield.
Simultaneous electrochemical determination of cadmium(II), lead(II), and mercury(II) ions in water and food samples using N1‐hydroxy‐N1,N2‐diphenylbenzamidine and multi‐walled carbon nanotubes modified carbon paste electrode
Authors: Tesfaye Endale , Singh Chandravanshi Bhagwan , Negash Negussie , Tessema Merid 
Published: September 2023

AbstractA novel modified carbon paste electrode has been developed using a laboratory synthesized metal chelating ligand (L), N1‐hydroxy‐N1,N2‐diphenylbenzamidine (HDPBA) and multi‐walled carbon nanotubes (MWCNTs) composite (L‐MWCNTs/CPE). The developed modified electrode was applied for selective and sensitive simultaneous electrochemical determination of cadmium(II), lead(II), and mercury(II) using square wave anodic stripping voltammetry (SWASV). The accumulation of metal ions on the electrode surface was performed in 0.1 M acetate buffer solution (ABS, pH 5.0) by applying a potential of–1.10 V versus Ag/AgCl for 210 s, followed by stripping in the positive potential scan of the SWASV after a rest time of 10 s. Under the optimum experimental conditions, the linear dynamic ranges were found to be 2×10−4–2, 1×10−4–2 and 2×10−3–2 μM of cadmium(II), lead(II), and mercury(II), respectively, with the detection limits (LODs) of 2.5×10−2, 7.8×10−3 and 4.6×10−1 nM for cadmium(II), lead(II), and mercury(II), respectively. The proposed electrode exhibited high sensitivity for the determination of these metal ions with excellent reproducibility and stability. The influence of several ions on the simultaneous determination of Cd(II), Pb(II), and Hg(II) was studied, and the prepared electrode was found to be highly selective for the detection of the three metal ions. The developed electrode was effectively applied to the determination of the three metal ions in water, vegetable and tobacco samples, and the obtained results were similar to those measured with graphite furnace atomic absorption spectrometry (GFAAS).