bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells
Authors: Davison Claire , Beste Dany , Bailey Melanie , Felipe-Sotelo Mónica 
Published: May 2023

AbstractMetals have a fundamental role in microbiology, and accurate methods are needed for their identification and quantification. The inability to assess cellular heterogeneity is considered an impediment to the successful treatment of different diseases. Unlike bulk approaches, single-cell analysis allows elemental heterogeneity across genetically identical populations to be related to specific biological events and to the effectiveness of drugs. Single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) can analyse single cells in suspension and measure this heterogeneity. Here we explore advances in instrumental design, compare mass analysers and discuss key parameters requiring optimisation. This review has identified that the effect of pre-treatment of cell suspensions and cell fixation approaches require further study and novel validation methods are needed as using bulk measurements is unsatisfactory. SP-ICP-MS has the advantage that a large number of cells can be analysed; however, it does not provide spatial information. Techniques based on laser ablation (LA) enable elemental mapping at the single-cell level, such as laser-induced breakdown spectroscopy (LIBS) and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The sensitivity of commercial LIBS instruments restricts its use for sub-tissue applications; however, the capacity to analyse endogenous bulk components paired with developments in nano-LIBS technology shows great potential for cellular research. LA-ICP-MS offers high sensitivity for the direct analysis of single cells, but standardisation requires further development. The hyphenation of these trace elemental analysis techniques and their coupling with multi-omic technologies for single-cell analysis have enormous potential in answering fundamental biological questions.
Single-cell ICP-MS for studying the association of inorganic nanoparticles with cell lines derived from aquaculture species
Authors: Suárez-Oubiña Cristian , Herbello-Hermelo Paloma , Mallo Natalia , Vázquez María , Cabaleiro Santiago , Pinheiro Ivone , Rodríguez-Lorenzo Laura , Espiña Begoña , Bermejo-Barrera Pilar , Moreda-Piñeiro Antonio 
Published: May 2023

AbstractThe current research deals with the use of single-cell inductively coupled plasma-mass spectrometry (scICP-MS) for the assessment of titanium dioxide nanoparticle (TiO2 NP) and silver nanoparticle (Ag NP) associations in cell lines derived from aquaculture species (sea bass, sea bream, and clams). The optimization studies have considered the avoidance of high dissolved background, multi-cell peak coincidence, and possible spectral interferences. Optimum operating conditions were found when using a dwell time of 50 μs for silver and 100 μs for titanium. The assessment of associated TiO2 NPs by scICP-MS required the use of ammonia as a reaction gas (flow rate at 0.75 mL min−1) for interference-free titanium determinations (measurements at an m/z ratio of 131 from the 48Ti(NH)(NH3)4 adduct). The influence of other parameters such as the number of washing cycles and the cell concentration on accurate determinations by scICP-MS was also fully investigated. Cell exposure trials were performed using PVP-Ag NPs (15 and 100 nm, nominal diameter) and citrate-TiO2 NPs (5, 25, and 45 nm, nominal diameter) at nominal concentrations of 10 and 50 μg mL−1 for citrate-TiO2 NPs and 5.0 and 50 μg mL−1 for PVP-Ag NPs. Results have shown that citrate-TiO2 NPs interact with the outer cell membranes, being quite low in the number of citrate-TiO2 NPs that enters the cells (the high degree of aggregation is the main factor which leads to the aggregates being in the extracellular medium). In contrast, PVP-Ag NPs have been found to enter the cells.
Graphical abstract
A review of the importance of south-east Australian waters as a global hotspot for leatherback turtle foraging and entanglement threat in fisheries
Authors: Hays Graeme C. , Morrice Margie , Tromp Jared J. 
Published: May 2023

AbstractAustralia’s largest sea turtle is the leatherback (Dermochelys coriacea). Leatherbacks do not nest, or only rarely, in Australia, and hence receive relatively little research attention. Here we review the knowledge of leatherback turtle occurrence in south-east (SE) Australia, drawing on sightings information as well as satellite tracking data from turtles equipped at their nesting beaches in Indonesia, the Solomon Islands and Papua New Guinea that then travelled to Australia. These data reveal that SE Australia likely provides a globally important foraging area for this species. Sea turtle temperatures assigned to sightings of live leatherbacks, showed 95% were seen at SSTs ≥ 14 °C. Similar to other parts of the world, such as the North Atlantic, the 12–15 °C isotherms likely constrain the seasonal pole-wards migration of leatherbacks searching for their gelatinous prey. Climate warming is likely moving the foraging range of leatherbacks poleward. This study also highlights the vulnerability of this SE Australian population to anthropogenic threats. Of 605 sightings of leatherbacks, 11.6% were of dead individuals, generally washed ashore, in most cases likely after entanglement in fishing gear.
Artificial intelligence tools in clinical neuroradiology: essential medico-legal aspects
Authors: Hedderich Dennis M. , Weisstanner Christian , Van Cauter Sofie , Federau Christian , Edjlali Myriam , Radbruch Alexander , Gerke Sara , Haller Sven 
Published: May 2023

AbstractCommercial software based on artificial intelligence (AI) is entering clinical practice in neuroradiology. Consequently, medico-legal aspects of using Software as a Medical Device (SaMD) become increasingly important. These medico-legal issues warrant an interdisciplinary approach and may affect the way we work in daily practice. In this article, we seek to address three major topics: medical malpractice liability, regulation of AI-based medical devices, and privacy protection in shared medical imaging data, thereby focusing on the legal frameworks of the European Union and the USA. As many of the presented concepts are very complex and, in part, remain yet unsolved, this article is not meant to be comprehensive but rather thought-provoking. The goal is to engage clinical neuroradiologists in the debate and equip them to actively shape these topics in the future.
Improved predictability of pancreatic ductal adenocarcinoma diagnosis using a blood immune cell biomarker panel developed from bulk mRNA sequencing and single-cell RNA-sequencing
Authors: Jang Sung Ill , Lee Hyung Keun , Chang Eun-Ju , Kim Somi , Kim So Young , Hong In Young , Kim Jong Kyoung , Lee Hye Sun , Yang Juyeon , Cho Jae Hee , Lee Dong Ki 
Published: May 2023

Abstract
Background
Pancreatic ductal adenocarcinoma (PDAC) remains a devastating cancer due to its poor survival rate, early detection, and resectability. This study aimed to determine the peripheral blood mononuclear cell (PBMC) immune biomarkers in patients with PDAC and investigate the PDAC-specific peripheral blood biomarker panel and validate its clinical performance.

Methods
In this prospective, blinded, case–control study, a biomarker panel formula was generated using a development cohort—including healthy controls, patients at high risk of PDAC, and patients with benign pancreatic disease, PDAC, or other gastrointestinal malignancies—and its diagnostic performance was verified using a validation cohort, including patients with ≥ 1 lesion suspected as PDAC on computed tomography (CT).

Results
RNA-sequencing of PBMCs from patients with PDAC identified three novel immune cell markers, IL-7R, PLD4, and ID3, as specific markers for PDAC. Regarding the diagnostic performance of the regression formula for the three biomarker panels, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 84.0%, 78.8%, 47.2%, 95.6%, and 79.8%, respectively. Based on the formula scores for the biomarker panel, the false-negative rate (FNR) of the biomarkers was 8% (95% confidence interval [CI] 3.0–13.0), which was significantly lower than that based on CT in the validation cohort (29.2%, 95% CI 20.8–37.6).

Conclusions
The regression formula constructed using three PBMC biomarkers is an inexpensive, rapid, and convenient method that shows clinically useful performance for the diagnosis of PDAC. It aids diagnoses and differential diagnoses of PDAC from pancreatic disease by lowering the FNR compared to CT.
Clinical trial registration Clinical Research Information Service, KCT0004614 (08 January 2020).
Exploiting docetaxel-induced tumor cell necrosis with tumor targeted delivery of IL-12
Authors: Franks S. Elizabeth , Santiago-Sanchez Ginette S. , Fabian Kellsye P. , Solocinski Kristen , Chariou Paul L. , Hamilton Duane H. , Kowalczyk Joshua T. , Padget Michelle R. , Gameiro Sofia R. , Schlom Jeffrey , Hodge James W. 
Published: May 2023

AbstractThere is strong evidence that chemotherapy can induce tumor necrosis which can be exploited for the targeted delivery of immuno-oncology agents into the tumor microenvironment (TME). We hypothesized that docetaxel, a chemotherapeutic agent that induces necrosis, in combination with the bifunctional molecule NHS-IL-12 (M9241), which delivers recombinant IL-12 through specific targeting of necrotic regions in the tumor, would provide a significant antitumor benefit in the poorly inflamed murine tumor model, EMT6 (breast), and in the moderately immune-infiltrated tumor model, MC38 (colorectal). Docetaxel, as monotherapy or in combination with NHS-IL-12, promoted tumor necrosis, leading to the improved accumulation and retention of NHS-IL-12 in the TME. Significant antitumor activity and prolonged survival were observed in cohorts receiving docetaxel and NHS-IL-12 combination therapy in both the MC38 and EMT6 murine models. The therapeutic effects were associated with increased tumor infiltrating lymphocytes and were dependent on CD8+ T cells. Transcriptomics of the TME of mice receiving the combination therapy revealed the upregulation of genes involving crosstalk between innate and adaptive immunity factors, as well as the downregulation of signatures of myeloid cells. In addition, docetaxel and NHS-IL-12 combination therapy effectively controlled tumor growth of PD-L1 wild-type and PD-L1 knockout MC38 in vivo, implying this combination could be applied in immune checkpoint refractory tumors, and/or tumors regardless of PD-L1 status. The data presented herein provide the rationale for the design of clinical studies employing this combination or similar combinations of agents.
A Scoping Review of Kidney Transplantation in Africa: How Far have We Come?
Authors: Davis Samuel Olawale , Zubair Abdulahi , Igbokwe Martin , Abu Moses , Chiedozie Chineme‐Anyaeze , Sanni Quadri , Jesuyajolu Damilola 
Published: May 2023

AbstractIntroductionKidney transplantation is a life‐saving treatment for end‐stage kidney disease (ESKD) patients. However, access to this treatment in Africa lags behind other regions, leading to significant disparities in care. We aimed to analyse the indications, demographics, and outcomes of kidney transplantation in Africa.
MethodWe conducted a systematic review of studies from PubMed, Google Scholar, and African Journal Online using the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guidelines. We pooled and analysed data on procedure type, graft outcomes, donor type, prophylaxis, post‐operative complications, and survival from 33 eligible studies.
ResultThe most common causes of ESKD requiring transplantation were glomerulonephritis and nephroangiosclerosis. Open nephrectomy was the predominant surgical approach (95%). Living donors accounted for 56.3% (4221) of all donors, with 68.5% being related to the recipient. Cadaveric donors accounted for 43.7% (3280) of transplants. Graft rejection was the most common surgical complication (39.44%), and 60.49% of patients developed hypertension in the follow‐up period.ConclusionOur study highlights the potential of kidney transplantation to improve the lives of ESKD patients in Africa. However, further research and infrastructure development are necessary to make this treatment more widespread and successful.
One-stage product-line design heuristics: an empirical comparison
Authors: Baier Daniel , Voekler Sascha 
Published: May 2023

AbstractSelecting or adjusting attribute-levels (e.g. components, equipments, flavors, ingredients, prices, tastes) for multiple new and/or status quo products is an important task for a focal firm in a dynamic market. Usually, the goal is to maximize expected overall buyers’ welfare based on consumers’ partworths or expected revenue, market share, and profit under given assumptions. However, in general, these so-called product-line design problems cannot be solved exactly in acceptable computing time. Therefore, heuristics have been proposed: Two-stage heuristics select promising candidates for single products and evaluate sets of them as product-lines. One-stage heuristics directly search for multiple attribute-level combinations. In this paper, Ant Colony Optimization, Genetic Algorithms, Particle Swarm Optimization, Simulated Annealing and, firstly, Cluster-based Genetic Algorithm and Max-Min Ant Systems are applied to 78 small- to large-size product-line design problem instances. In contrast to former comparisons, data is generated according to a large sample of commercial conjoint analysis applications (n = 2,089). The results are promising: The firstly applied heuristics outperform the established ones.
Biomineralisation and metal sequestration in a crustacean ectoparasite infecting the gills of a freshwater fish
Authors: Latief Lutfiyya , Gilbert Beric M. , Avenant-Oldewage Annemariè 
Published: May 2023

AbstractIt has been suggested that parasites are effective bioindicators as they are sensitive to environmental changes and, in some cases, accumulate trace elements in higher concentrations than their hosts. Accumulated elements sequester in different organs. In monogenean and crustacean ectoparasites, sclerotised structures and egg yolk appear to be the preferred site for element sequestration. In this study, the sequestration of trace elements; Mg, Al, Ca, Fe, Cu, and Zn in Lamproglena clariae was studied from two rivers. Adult L. clariae were collected from the gills of Clarias gariepinus from Lake Heritage in the Crocodile River and in the Vaal River below the Vaal Dam, South Africa. Collected parasites were flash frozen in liquid nitrogen and sectioned with a cryomicrotome. Sections were treated with Phen-Green to observe fluorescent signals. Trace elements in the parasite were analysed using a scanning electron microscope with an energy-dispersive spectroscope (SEM–EDS). Results showed more intense fluorescence signals in the exoskeleton compared to tissues, and in the egg yolk. Analysis by SEM–EDS confirmed the presence of elements in the parasite from both sites. Levels of Al were higher in L. clariae from the Vaal River than those from Lake Heritage, and Fe was higher in L. clariae from Lake Heritage. Element distribution patterns in the parasite matched those in the water from the sites. Unlike other crustaceans, regulation of metals in adult females of L. clariae does not occur through moulting, but high levels occurred in the yolk.
Drivers of low-frequency Sahel precipitation variability: comparing CMIP5 and CMIP6 ensemble means with observations
Authors: Herman Rebecca Jean , Biasutti Michela , Kushnir Yochanan 
Published: May 2023

AbstractPhases 5 and 6 of the Coupled Model Intercomparison Project (CMIP5 and CMIP6) both grossly underestimate the magnitude of low-frequency Sahel rainfall variability; but unlike CMIP5, CMIP6 mean historical precipitation does not even correlate with observed multi-decadal variability. We demarcate realms of simulated physical processes that may induce differences between these ensembles and prevent both from explaining observations. We partition all influences on simulated Sahelian precipitation variability into (1) teleconnections from sea surface temperature (SST); (2) atmospheric and (3) oceanic variability internal to the climate system; (4) the SST response to external radiative forcing; and (5) the “fast” (not mediated by SST) precipitation response to radiative forcing. In a vast improvement from previous ensembles, the mean spectral power of Sahel rainfall in CMIP6 atmosphere-only simulations is consistent with observed low-frequency variance. Low-frequency variability is dominated by teleconnections from observed global SST, and the fast response only hurts the performance of simulated precipitation. We estimate that the strength of simulated teleconnections is consistent with observations using the previously-established North Atlantic Relative Index (NARI) to approximate the role of global SST, and apply this relationship to the coupled ensembles to infer that both fail to explain low-frequency historical Sahel rainfall variability mostly because they cannot explain the observed combination of forced and internal variability in North Atlantic SST. Yet differences between CMIP5 and CMIP6 in mean Sahel precipitation and its correlation with observations do not derive from differences in NARI, but from the fast response or the role of other SST patterns.
Age and gender in gerontechnology development
Authors: Rießenberger Katja Antonia , Fischer Florian 
Published: May 2023

Abstract
Background
The challenges of the digital divide emerge with new technologies being created to address the needs of the increasing older population. This divide is influenced by the social dimensions of age and gender, often resulting in impaired participation of the affected demographic groups. Gerontechnological designs in which inadequate attention is paid to gender and old age easily run the risk of reproducing gender-specific and age-specific stereotypes. An approach to counteracting the digital exclusion of technology users is the introduction of co-creative methods of participatory design (PD). As there are diverse challenges when putting these methods into practice regarding their claim to be more socially inclusive and democratizing technology development, it is necessary to investigate the effect that age and gender could play when considering PD in gerontechnology.

Objective
This article aims to shed light on the intersection of age and gender as dimensions of horizontal inequalities in gerontechnology development to support the further development of co-creation practices.

Conclusion
The PD approaches can be regarded as suitable methodologies to descript age and gender in technology development as long as they are enhanced by a critical awareness of gendered and ageist patterns in society and technology development. The intersectional approach can contribute to further understanding of how current gerontechnology development practices promote the reinforcement and challenging of dominant discourses on old age and gender.
Characteristics of smell and taste disorders depending on etiology: a retrospective study
Authors: Trache Mihnea Cristian , Schipp Josef Maria Heinrich , Haack Mareike , Adderson-Kisser Christine , Högerle Catalina , Becker Sven , Betz Christian Stephan 
Published: May 2023

Abstract
Purpose
This study investigates the impact of etiology on the epidemiologic profile, disease severity, type of treatment and therapy outcome in smell and taste disorders.

Methods
This is a retrospective analysis of 270 patients that presented with a smell or taste disorder in a specialized, tertiary care center. An established questionnaire was used to collect data from patients and physicians. Olfactometry was performed with the Sniffin’ Sticks test kit, while gustometry was performed by taste strips.

Results
Post-traumatic etiology was associated with young age (median 46 years) and male sex, and showed the most severe degrees of smell loss compared to other etiologies (64.3% anosmia). Postinfectious causes occurred more frequently in females (77.3%) and correlated with a history of pharyngeal surgery, suggesting a vulnerability for virally mediated sensory dysfunction following adenoid/tonsil removal. Parosmia also correlated with both postinfectious etiology (62.5%) and female sex. In sinunasal etiology, the presence of nasal polyps worsened the overall olfactory test score by approximately 50%. In particular, smell threshold and discrimination were reduced, while smell identification was not significantly impacted by nasal polyp obstruction. Sinunasal dysfunction was the only etiology to show significant improvement after therapy (73.9% improved). Finally, we could establish good correlations between the subjective impairment and objective dysfunction for each sensory modality.

Conclusion
Each etiology of chemosensory dysfunction shows particular distributions of variables like sex, age, comorbidities and operations, disease severity, sensory threshold, discrimination and identification. This paper offers a detailed account of the correlations between the cause and the characteristics of smell and taste loss.
Alcohol and the vasculature: a love-hate relationship?
Authors: Li Huige , Xia Ning 
Published: May 2023

AbstractAlcohol consumption is a leading risk factor and increases the risk of liver diseases, cancers, tuberculosis, and injuries. The relationship between alcohol use and cardiovascular risk is complex. While it is well established that heavy alcohol use and binge drinking harm cardiovascular health, the effect of light-to-moderate alcohol consumption remains controversial. Observational studies have repeatedly confirmed the U- or J-shaped relationship between alcohol consumption and cardiovascular disease risk, with the lowest risk observed in the light-to-moderate drinking group. However, the protective effect of low-level alcohol has been challenged by recent genetic epidemiological studies with Mendelian randomization. Such studies have their own limitations, and the application of this methodology in studying alcohol has been questioned. Results from the latest Global Burden of Diseases, Injuries, and Risk Factors Study suggest that the impact of alcohol consumption on health depends on the age structure and the distribution of disease burden and underlying causes in a given population. For young adults, even small amounts of alcohol cause heath loss. For older adults facing a high burden of cardiovascular diseases, light-to-moderate alcohol consumption may improve cardiovascular health outcomes. Mechanistically, all types of alcoholic beverages, including wine, spirits, and beer, have been shown to increase the levels of high-density lipoprotein cholesterol and adiponectin, and reduce the level of fibrinogen. Nonalcoholic components of wine, especially polyphenolic compounds like resveratrol, may additionally enhance endothelial nitric oxide production, and provide antioxidant and anti-inflammatory effects.