bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reference values of activated partial thromboplastin time, Quick`s value, INR, thrombin time, fibrinogen, antithrombin and II, V, VII, VIII, IX, X, XI and XII coagulation factors determined with automated Sysmex CS-2000i analyzer
Authors: Gorgidze L. А. , Mamleeva S. Yu. , Pimenov М. S. , Smirnova А. V. , Bulgakov А. V. , Galstyan G. М. 
Published: March 2023

The article defines reference values for activated partial thromboplastin time, Quick’s value, INR, thrombin time, fibrinogen, antithrombin and II, V, VII, VIII, IX, X, XI and XII coagulation factors, according to existing standards on the automated Sysmex CS‑2000i analyzer.The aim of the study. To determine reference values for routine and specific parameters of the hemostasis, which may vary depending on the type of analyzer and utilized reagents.Materials and methods. After receiving informed consent from donors for medical survey and blood donation, blood samples were obtained from 100 healthy donors: 64 (64%) males и 36 (36%) females. We established reference values with the Sysmex CS‑2000i (Sysmex, Japan) hemostasis analyzer and reagents from Siemens (Siemens Healthcare, Germany).Results. The data obtained were compared with the literature data and the data presented in the instructions for the reagents used. The results obtained for activated partial thromboplastin time (23.59–35.69 sec), fibrinogen (1.67–3.59 g/l) and antithrombin (67.65–114.89%) are comparable to the available data. There are no data on other studied parameters of hemostasis for the Sysmex CS‑2000i analyzer and the reagents used in the work. The obtained reference intervals are consistent with the recommendations of the manufacturer.Conclusions. Reference values vary significantly depending on the analytical systems and reagent kits used, which confirms the need for local derivation or validation of reference intervals for each specific analytical system and in each laboratory.
Sampling algorithm for geno- and subtyping of hepatitis B virus using panel of monoclonal antibodies in enzyme immunoassay
Authors: Bezuglova L. V. , Potapova A. A. , Netesova I. G. 
Published: March 2023

Introduction. Previously, a domestic method for determining hepatitis B virus (HBV) genotypes and HBV surface antigen (HBsAg) subtypes in HBsAgpositive blood serum samples with a Monoclonal Antibody Panel (MAB) by enzyme immunoassay (ELISA) was described. In routine laboratory practice, HBsAg detection results are obtained in units of optical density (OD).Purpose. To describe the sampling algorithm for ELISA geno- and subtyping of HBV with the MAB Panel.Materials and methods. We studied 40 blood serum samples with positive results of HBsAg determination. HBV genotypes and HBsAg subtypes were determined using four different MAB conjugates with horseradish peroxidase using the ELISA method described previously.Results. In samples with OD less than 2.0 o. u. and confirmation of HBsAg after a single confirmatory study (n = 19) HBV genotypes/ HBsAg subtypes not established. In samples with an OD above 2.0 o. u. and verification of the presence of the antigen in the standard mode of registration (n = 15), immunoenzymatic geno- and subtyping of HBV was effective in 27 % of cases (4/15); for samples with HBsAg verification carried out in an auxiliary measurement (n = 6) the effectiveness of the technique was 100 %. Using the MAB panel in ELISA, the characteristics of HBV in 10 samples with the presence of HBsAg were studied: HBV genotype D (n = 10), subtypes ayw2 (n = 7), ayw3 (n = 3).Conclusions. For the most effective application of the HBV geno- and subtyping technique using the MAB Panel in ELISA, HBsAg+ samples with OD signals of more than 2.0 o. u. in screening and verification of the presence of an antigen in the auxiliary mode of registration of results should be used.
PENDAMPINGAN PEMBUATAN RENCANA PELAKSANAAN PEMBELAJARAN BAGI GURU PPKn SMA DI KABUPATEN OGAN KOMERING ILIR
Authors: Edwin Nurdiansyah  , Kurnisar  , Umi Chotimah  , Faisal Emil 
Published: March 2023

Beberapa tahun sebelumnya kita menggunakan RRP 13 komponen berdasarkan Permendikbud No 22 Tahun 2016 tentang Standar Proses yang berisikan: Identitas mata pelajaran; Standar kompetensi; Kompetensi dasar; Indikator pencapaian kompetensi; Tujuan pembelajaran; Materi ajar; Alokasi waktu; Metode pembelajaran; Kegiatan pembelajaran; Penilaian hasil belajar; Sumber belajar. Sedangkan sekarang dengan adanya program merdeka belajar maka Menteri Pendidikan dan kebudayaan membuat kebijakan dengan RPP cukup 1 Lembar. Hal tersebut bertujuan untuk menyederhankan 13 komponen menjadi 3 komponen saja yang dianggap paling penting meliputi tujuan, kegiatan dan penilaian pembelajaran. Pelatihan yang dilakukan bertujuan agar guru mampu membuat RPP sesuai dengan pedoman yang ada. Pelatihan dilakukan secara daring melalui zoom meeting karena masih dalam keadaan pandemi sehingga belum bisa dilakukan secara tatap muka. Peserta berjumlah 20 orang guru yang tergabung dalam MGMP (Musyawarah Guru Mata Pelajaran) PPKn Kabupaten Ogan Komering Ilir (OKI). Dari hasil pelatihan terjadi peningkatan pemahaman sebelum dan sesudah pelatihan, ini ditandai dengan adanya peningkatan pada skor tes yang sebelumnya rerata 47 menjadi rerata 67 setelah pelatihan. Selain itu peserta pelatihan juga telah memiliki keterampilan dalam membuat RPP ini terlihat dari skor penilaian produk RPP dimana didapatkan rerata 93,6% dengan kategori sangat baik.
Kata kunci: Pelatihan, RPP, PPKn
PEMANFAATAN MEDIA SOSIAL SEBAGAI SARANA PROMOSI PADA BISNIS PEMPEK LESTARI
Authors: Ikhsani Ahmad Alif , Dhanar Intan Surya Saputra  
Published: March 2023

Internet development is happening very rapidly, and the exchange of information is becoming easier and faster. It makes people tend to use the internet more to communicate and interact. Social media has many benefits in everyday life, and doing business is no exception. For businesses, social media can be used as a tool to promote a product or service. The purpose of the activity is to find out how to use social media as a promotional media for a product so that consumers can reach it, what obstacles exist when using social media for the promotion of a food product, and what solutions should be done in utilizing social media as a promotional media. The method in this activity uses analysis and mentoring approach. The results of the action show that there are several obstacles to the use of social media as promotional media. Through this activity, these obstacles can be faced and resolved by the use of social media as a promotional medium by Dapoer Lestari. The solution is to use social media, Facebook, to promote the Pempek Dapoer Lestari business.
Key Words: Media Social, Promotions, Facebook
Corpo e ancestralidade: estudo dos rituais e mitos de origem afro"brasileira no panorama da dança contemporânea brasileira
Authors: Côrtes Gustavo , Santos Inaicyra Falcão dos , Andraus Mariana Baruco Machado 
Published: March 2023

Este artigo discute as possibilidades de apropriação do métododesenvolvido pela Profa. Dra. Inaicyra Falcão dos Santos, intitulado Corpo eAncestralidade, tematizado no Programa de Pós"Graduação em Artes da UniversidadeEstadual de Campinas. A partir de um percurso histórico, busca"se compreendercomo se estabelecem as relações entre as pesquisas teóricas e os processos criativosque geram performances relacionando rituais mí­ticos – especialmente os vinculados í cultura de origem negra – às possibilidades da dança contemporânea brasileira.Conclui"se que o trabalho de reconhecimento dos rituais e mitos como percursoartí­stico torna"se o principal elemento na construção de uma obrametodologicamente estruturada no contexto da proposta de pesquisa Corpo eAncestralidade.Palavras"chave: Ancestralidade. Mito. Dança brasileira. Processo de criação.
The study on the impact of AAA wall motion on the hemodynamics based on 4D CT image data
Authors: Peng Chen , He Wei , Huang Xingsheng , Ma Jun , Yuan Tong , Shi Yun , Wang Shengzhang 
Published: March 2023

Purpose: To analyze the effect of the physiological deformation of the vessel wall on the hemodynamics in the abdominal aortic aneurysm (AAA), this paper compared the hemodynamics in AAA based on the moving boundary (MB) simulation and the rigid wall (RW) simulation.Method: Patient-specific models were reconstructed to generate mesh based on four-dimensional computed tomography angiography (4D CT) data. The dynamic mesh technique was used to achieve deformation of the vessel wall, surface mesh and volume mesh of the fluid domain were successively remeshed at each time step. Besides, another rigid wall simulation was performed. Hemodynamics obtained from these two simulations were compared.Results: Flow field and wall shear stress (WSS) distribution are similar. When using the moving boundary method (MBM), mean time-averaged wall shear stress (TAWSS) is lower, mean oscillatory shear index (OSI) and mean relative residence time (RRT) are higher. When using the 10th and 20th percentile values for TAWSS and 80th and 90th percentile values for RRT, the ratios of areas with low TAWSS, high OSI and high RRT to the entire vessel wall are higher than those assuming the vessel as rigid. In addition, one overlapping region of low TAWSS, high OSI and high RRT by using the MBM is consistent with the location of thrombus obtained from the follow-up imaging data.Conclusion: The hemodynamics results by using the MBM reflect a higher blood retention effect. This paper presents a potential tool to assess the risk of intraluminal thrombus (ILT) formation based on the MBM.
Real-time calcium uptake monitoring of a single renal cancer cell based on an all-solid-state potentiometric microsensor
Authors: Zhai Jiali , Wang Wenting , Wu Shuang , Yu Tianxi , Xiang Chongjun , Li Yue , Lin Chunhua , Zhao Guangtao 
Published: March 2023

Introduction: In addition to many cellular processes, Ca2+ is also involved in tumor initiation, progression, angiogenesis, and metastasis. The development of new tools for single-cell Ca2+ measurement could open a new avenue for cancer therapy.Methods: The all-solid-state calcium ion-selective microelectrode (Ca2+-ISμE) based on carbon fiber modified with PEDOT (PSS) as solid-contact was developed in this work, and the characteristics of the Ca2+-ISμE have also been investigated.Results: The Ca2+-ISμE exhibits a stable Nernstian response in CaCl2 solutions in the active range of 1.0 × 10−8 - 3.1 × 10−3 M with a low detection limit of 8.9 × 10−9 M. The Ca2+-ISμE can be connected to a patch clamp to fabricate a single-cell analysis platform for in vivo calcium monitoring of a single renal carcinoma cell. The calcium signal decreased significantly (8.6 ± 3.2 mV, n = 3) with severe fluctuations of 5.9 ± 1.8 mV when the concentration of K+ in the tumor microenvironment is up to 20 mM.Discussion: The results indicate a severe cell response of a single renal carcinoma cell under high K+ stimuli. The detection system could also be used for single-cell analysis of other ions by changing different ion-selective membranes with high temporal resolution.
Mechanical assessment of two hybrid plate designs for pancarpal canine arthrodesis under cyclic loading
Authors: Zderic Ivan , Varga Peter , Styger Ursula , Drenchev Ludmil , Gueorguiev Boyko , Asimus Erik , Saunders W. Brian , Kowaleski Michael , Boudrieau Randy J. , Déjardin Loic M. 
Published: March 2023

Pancarpal canine arthrodesis (PCA) sets immobilization of all three carpal jointsviadorsal plating to result in bony fusion. Whereas the first version of the plate uses a round hole (RH) for the radiocarpal (RC) screw region, its modification into an oval hole (OH) in a later version improves versatility in surgical application. The aim of this study was to mechanically investigate the fatigue life of the PCA plate types implementing these two features–PCA-RH and PCA-OH. Ten PCA-RH and 20 PCA-OH stainless steel (316LVM) plates were assigned to three study groups (n = 10). All plates were pre-bent at 20° and fixed to a canine forelimb model with simulated radius, RC bone and third metacarpal bone. The OH plates were fixed with an RC screw inserted either most proximal (OH-P) or most distal (OH-D). All specimens were cyclically tested at 8 Hz under 320 N loading until failure. Fatigue life outcome measures were cycles to failure and failure mode. Cycles to failure were higher for RH plate fixation (695,264 ± 344,023)versusboth OH-P (447,900 ± 176,208) and OH-D (391,822 ± 165,116) plate configurations, being significantly different between RH and OH-D,p= 0.03. No significant difference was detected between OH-P and OH-D configurations,p= 0.09. Despite potential surgical advantages, the shorter fatigue life of the PCA-OH plate design may mitigate its benefits compared to the plate design with a round radiocarpal screw hole. Moreover, the failure risk of plates with an oval hole is increased regardless from the screw position in this hole. Based on these findings, the PCA plate with the current oval radiocarpal screw hole configuration cannot be recommended for clinical use.
Fullerenol inhibits tendinopathy by alleviating inflammation
Authors: Jiao Xin , Wang Zengguang , Li Yiming , Wang Tianchang , Xu Chen , Zhou Xianhao , Gan Yaokai 
Published: March 2023

Tendinopathy is a common disease in orthopaedics, seriously affecting tendon functions. However, the effects of non-surgical treatment on tendinopathy are not satisfactory and surgical treatments possibly impair the function of tendons. Biomaterial fullerenol has been proved to show good anti-inflammatory effects on various inflammatory diseases. For in vitro experiments, primary rat tendon cells (TCs) were treated by interleukin-1 beta (IL-1β) combined with aqueous fullerenol (5, 1, 0.3 μg/mL). Then inflammatory factors, tendon-related markers, migration and signaling pathways were detected. For in vivo experiments, rat tendinopathy model was constructed by local injection of collagenase into Achilles tendons of rats and fullerenol (0.5, 1 mg/mL) was locally injected 7 days after collagenase injection. Inflammatory factors and tendon-related markers were also investigated. Fullerenol with good water-solubility showed excellent biocompatibility with TCs. Fullerenol could increase expression of tendon-related factors (Collagen I and tenascin C) and decrease expression of inflammatory factors (matrix metalloproteinases-3, MMP-3, and MMP-13) and reactive oxygen species (ROS) level. Simultaneously, fullerenol slowed the migration of TCs and inhibited activation of Mitogen-activated protein kinase (MAPK) signaling pathway. Fullerenol also attenuated tendinopathy in vivo, including reduction of fiber disorders, decrease of inflammatory factors and increase of tendon markers. In summary, fullerenol is a promising biomaterial that can be used to treat tendinopathy.
iNOS expressing macrophages co-localize with nitrotyrosine staining after myocardial infarction in humans
Authors: Wilmes Verena , Kur Ivan M. , Weigert Andreas , Verhoff Marcel A. , Gradhand Elise , Kauferstein Silke 
Published: March 2023

IntroductionInducible nitric oxide synthase (iNOS) produces micromolar amounts of nitric oxide (NO) upon the right stimuli, whose further reactions can lead to oxidative stress. In murine models of myocardial infarction (MI), iNOS is known to be expressed in infiltrating macrophages, which at early onset enter the infarcted zone and are associated with inflammation. In contrast cardiac tissue resident macrophages are thought to enhance regeneration of tissue injury and re-establish homeostasis. Both detrimental and beneficial effects of iNOS have been described, still the role of iNOS in MI is not fully understood. Our aim was to examine cell expression patterns of iNOS and nitrotyrosine (NT) production in human MI.Material and MethodsWe examined in postmortem human MI hearts the iNOS mRNA expression by means of qPCR. Further we performed immunohistochemical stainings for cell type identification. Afterwards a distance analysis between iNOS and NT was carried out to determine causality between iNOS and NT production.ResultsiNOS mRNA expression was significantly increased in infarcted regions of human MI hearts and iNOS protein expression was detected in resident macrophages in infarcted human hearts as well as in controls hearts, being higher in resident macrophages in MI hearts compared to control. Furthermore in MI and in healthy human hearts cells showing signs of NT production peaked within 10–15 µm proximity of iNOS+ cells.DiscussionThese results indicate that, unexpectedly, resident macrophages are the main source of iNOS expression in postmortem human MI hearts. The peak of NT positive cells within 10–15 µm of iNOS+ cells suggest an iNOS dependent level of NT and therefore iNOS dependent oxidative stress. Our results contribute to understanding the role of iNOS in human MI.
Bibliometric and visual analysis of RAN methylation in cardiovascular disease
Authors: Song Boce , Xie Beili , Liu Mingwang , Li Haohao , Shi Dazhuo , Zhao Fuhai 
Published: March 2023

BackgroundRNA methylation is associated with cardiovascular disease (CVD) occurrence and development. The purpose of this study is to visually analyze the results and research trends of global RNA methylation in CVD.MethodsArticles and reviews on RNA methylation in CVD published before 6 November 2022 were searched in the Web of Science Core Collection. Visual and statistical analysis was performed using CiteSpace 1.6.R4 advanced and VOSviewer 1.6.18.ResultsThere were 847 papers from 1,188 institutions and 63 countries/regions. Over approximately 30 years, there was a gradual increase in publications and citations on RNA methylation in CVD. America and China had the highest output (284 and 259 papers, respectively). Nine of the top 20 institutions that published articles were from China, among which Fudan University represented the most. The International Journal of Molecular Sciences was the journal with the most studies. Nature was the most co-cited journal. The most influential writers were Zhang and Wang from China and Mathiyalagan from the United States. After 2015, the primary keywords were cardiac development, heart, promoter methylation, RNA methylation, and N6-methyladenosine. Nuclear RNA, m6A methylation, inhibition, and myocardial infarction were the most common burst keywords from 2020 to the present.ConclusionsA bibliometric analysis reveals research hotspots and trends of RNA methylation in CVD. The regulatory mechanisms of RNA methylation related to CVD and the clinical application of their results, especially m6A methylation, are likely to be the focus of future research.
A magnetic measurement technique for small rare samples: Pre-study for Chang’e-5 returned and other extraterrestrial samples
Authors: Hou Yifei , Zhang Min , He Kuang , Shen Zhongshan , Dong Liang , Fan Yunchang , Qi Kaixian , Liu Shuangchi , Cai Shuhui , Qin Huafeng 
Published: March 2023

Standard-size samples are usually used for traditional paleomagnetic studies. In some cases, small irregular samples are employed considering their amount and magnetic strength as well as experiment efficiency. With the development of deep space exploration, the demand for studying small and magnetic weak samples is increasing. In this study, we established a magnetic measurement technique for small rare samples. We designed low-background, sample-nondestructive and high-flexibility holders with various materials for different measurements of small samples. With the customized holders, we estimated uncertainty and reliability of magnetic measurements of small specimens from a terrestrial basalt. The results indicate that susceptibility and remanence of small specimens (e.g., 2-mm cubes) are more affected by measurement position than larger specimens (≥5 mm), which reminds us to pay special attention to consistency of the small specimen position during series of measurements. Moreover, susceptibility and remanence of small sister specimens (2-mm and 5-mm cubes) are more scattered than those of large ones (cube length ≥1 cm), mainly due to inhomogeneity of magnetic mineral abundance in the sample. Nevertheless, the average magnetic results of different-size specimens are generally consistent within error, indicating the inhomogeneity of small specimens can be averaged out and demonstrating the reliability of small samples during magnetic measurements. Finally, we tested our measurement system with a lunar meteorite, which mimics the Chang’e-5 returned samples in both size and mineralogy. The main minerals of the meteorite are plagioclase, pyroxene, troilite and kamacite. The isothermal remanent magnetization normalized paleointensity method recovered the expected laboratory field while the anhysteretic remanent magnetization corrected paleointensities are about 2-3 times the expected field, where the latter can be explained by inapplicability of the empirical f′ to the meteorite specimens. The anhysteretic remanent magnetization and IRM paleointensity calibration factor f′ and a of the lunar meteorite were calculated to be 3.89 and 3138 μT, respectively, which provides new constraints for the empirical values. The magnetic results of the lunar meteorite demonstrate the utility of our measurement system for small weak samples. This study provides technical support for paleomagnetic study of the Chang’e-5 and other extraterrestrial samples in the future.
Osteocalcin modulates parathyroid cell function in human parathyroid tumors
Authors: Verdelli Chiara , Tavanti Giulia Stefania , Forno Irene , Vaira Valentina , Maggiore Riccardo , Vicentini Leonardo , Dalino Ciaramella Paolo , Perticone Francesca , Lombardi Giovanni , Corbetta Sabrina 
Published: March 2023

IntroductionThe bone matrix protein osteocalcin (OC), secreted by osteoblasts, displays endocrine effects. We tested the hypothesis that OC modulates parathyroid tumor cell function.MethodsPrimary cell cultures derived from parathyroid adenomas (PAds) and HEK293 cells transiently transfected with the putative OC receptor GPRC6A or the calcium sensing receptor (CASR) were used as experimental models to investigate γ-carboxylated OC (GlaOC) or uncarboxylated OC (GluOC) modulation of intracellular signaling.ResultsIn primary cell cultures derived from PAds, incubation with GlaOC or GluOC modulated intracellular signaling, inhibiting pERK/ERK and increasing active β-catenin levels. GlaOC increased the expression of PTH, CCND1 and CASR, and reduced CDKN1B/p27 and TP73. GluOC stimulated transcription of PTH, and inhibited MEN1 expression. Moreover, GlaOC and GluOC reduced staurosporin-induced caspase 3/7 activity. The putative OC receptor GPRC6A was detected in normal and tumor parathyroids at membrane or cytoplasmic level in cells scattered throughout the parenchyma. In PAds, the membrane expression levels of GPRC6A and its closest homolog CASR positively correlated; GPRC6A protein levels positively correlated with circulating ionized and total calcium, and PTH levels of the patients harboring the analyzed PAds. Using HEK293A transiently transfected with either GPRC6A or CASR, and PAds-derived cells silenced for CASR, we showed that GlaOC and GluOC modulated pERK/ERK and active β-catenin mainly through CASR activation.ConclusionParathyroid gland emerges as a novel target of the bone secreted hormone osteocalcin, which may modulate tumor parathyroid CASR sensitivity and parathyroid cell apoptosis.