bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Median, Intermediate, and Lateral Approach in the Ultrasound-Guided Sacral Erector Spinae Plane Block: A Cadaveric and Radiologic Study
Authors: KELEŞ Bilge OLGUN , SALMAN Necati , YILMAZ Elvan TEKİR , BİRİNCİ Habip Resul , APAN Alparslan , İNCE Selami , ÖZYAŞAR Ali Faruk , UZ Aysun 
Published: April 2023

Abstract
Backgrounds: Erector spinae plane block (ESPB) has become very popular for post-operative and chronic pain management. It is aimed to observe the contrast agent distributions of three different methods anatomically and radiologically in ultrasound-guided sacral ESPB interventions.Materials and Methods: Six cadavers were enrolled and they were grouped into three groups; median, intermediate, and lateral approaches. An ultrasound-guided ESPB was performed with a mixture of radio-opaque and dye. After 30 minutes, the distribution of the solution was observed in the cadavers by computed tomography (CT) examination. Totally after 60 minutes of intervention, the median and intermediate groups of cadavers were dissected in order to observe the dye spread in the median group and the intermediate group. The lateral group dissection wasn’t performed according to radiologic findings.Results: CT images presented a pooling of the radio-opaque at subcutaneous tissue in the median group, at erector spinae in the intermediate group, and at gluteus maximus in the lateral group of cadavers. In addition to compartment spreads, images presented anterior transition of the contrast agent at only median group cadavers. Dissection findings presented pooling of the methylene blue at subcutaneous tissue between S1 and S5 horizontal planes at the median group. The red acrylic paint spread was detected between L2-S3 levels in the erector spinae muscle group.Conclusion: Radiocontrast dye was detected at superficial, erector spinae, and gluteus maximus compartments respectively at median, intermediate, and lateral cadaver groups both radiological and anatomically. Anterior transition of the radiocontrast was detected in only the median cadaver group.
Camrelizumab plus taxanes and cisplatin (TP) chemotherapy or TP chemotherapy in patients with recurrent or metastatic nasopharyngeal carcinoma
Authors: Liu Jingyu , Bao Yuqing , Zhu Lisheng , Xiong Ying , Shi Liangliang , Hong Xiaohua , Peng Gang 
Published: April 2023

Abstract

BackgroundThe addition of immune checkpoint inhibitors (ICIs) to platinum-based chemotherapy has shown promising antitumor activity in patients with recurrent or metastatic nasopharyngeal carcinoma (RM-NPC), who have poor survival outcomes. We therefore compared the efficacy and adverse events of taxanes-cisplatin (TP) chemotherapy and camrelizumab plus TP chemotherapy in patients with RM-NPC.Materials and methodsIn this retrospective study, we collected the medical records of 194 patients with RM-NPC between June 30, 2019, and December 31, 2021. The patients received camrelizumab plus TP chemotherapy or TP chemotherapy alone as first-line treatment at Wuhan Union Hospital Cancer Center. The survival outcomes, efficacy, and treatment-related adverse reactions were compared between the groups.ResultsThe medical records of 194 patients with RM-NPC were reviewed. Compared with the TP group, patients in the camrelizumab plus TP group had a longer median progression-free survival (PFS) (13.4 vs. 9.5 months; hazard ratio (HR) 0.628 [95% confidence interval (CI) 0.432–0.912];P = 0.015) and overall survival (OS) (73.91% vs. 57.84%; HR 0.544 [95% CI 0.336–0.878];P = 0.013). The results of multivariate analysis indicated that Epstein-Barr virus DNA (EBV DNA) load in plasma before treatment was an independent prognostic indicator associated with PFS (HR 0.488 [95% CI 0.336–0.709];P < 0.001) and OS (HR 0.602 [95% CI 0.372–0.976];P = 0.040)ConclusionOur study revealed that adding camrelizumab to taxanes-cisplatin chemotherapy showed a higher PFS and OS in patients with recurrent or metastatic nasopharyngeal carcinoma and had a manageable safety profile.
A Robust Fish Species Classification Framework: FRCNN-VGG16-SPPNet
Authors: Chen Mei-Hsin , Lai Ting-Hsuan , Chen Yao-Chung , Chou Tien-Yin , Ning Fang-Shii 
Published: April 2023

Abstract
This study proposes a novel framework for fish species classification that combines FRCNN (Faster Region-based Convolutional Neural Network), VGG16 (Visual Geometry Group 16), and SPPNet (Spatial Pyramid Pooling network). The proposed FRCNN-VGG16-SPPNet framework combines the strengths of FRCNN's fast object detection and localization, VGG16's convenient transfer learning and fast classification performance, and SPPNet's image processing flexibility and robustness in handling input images of any size. First, FRCNN is used to detect and extract target objects from images containing multiple objects. Subsequently, photos of various fish species at different scales are fed into VGG16-SPPNet, which performs basic feature extraction using transfer learning theory. SPPNet further processes the input images by performing pooling operations of different scales. Finally, VGG16 identifies important features to perform object classification. The proposed framework achieves higher accuracy compared to traditional single VGG16 models, particularly in classifying objects of different sizes, with an accuracy rate of 0.9318, which is 26% higher than traditional single VGG16 models. The proposed framework is efficient, convenient, reliable, and robust for object classification and has potential for various applications in image recognition and classification.
Isolation and identification of native yeasts from the spontaneous fermentation of grape musts.
Authors: Gerard Liliana Mabel , Corrado María Belén , Davies Cristina Verónica , Soldá Carina Alejandra , Dalzotto María Gabriela , Esteche Sofía 
Published: April 2023

Abstract
Recently, there has been growing interest in the characterization of native yeasts for their use in production of wines with regional characteristics. This study aimed to investigate Saccharomyces and non-Saccharomyces yeasts present in the spontaneous fermentation of Tannat and Marselan grape musts collected from Concordia (Entre Ríos, Argentina) over 2019, 2020, and 2021 vintages. The evolution of these fermentative processes was carried out by measuring total soluble solids, total acidity, volatile acidity, pH, ethanol concentration, and total carbon content. Isolated Saccharomyces and non-Saccharomyces yeasts were identified based on colony morphology in WL medium, 5.8S-ITS-RFLP analysis, and 26S rDNA D1/D2 gene sequencing. Two hundred and ten yeast colonies were isolated and identified as Pichia kudriavzevii, Saccharomyces cerevisiae, Hanseniaspora uvarum, Metschnikowia pulcherrima, Candida albicans, Candida parasilopsis, Pichia occidentalis, Pichia bruneiensis, Hanseniaspora opuntiae, Issatchenkia terricola, and Hanseniaspora vineae. P. kudriavzevii isolated from all vintages was associated with the spontaneous fermentation of grape musts from the Concordia region.
Almost a Hilbert Space
Authors: Mupasiri Douglas , G{\"u}l Erdal , Williams Daniel , Gill Tepper 
Published: April 2023

Abstract
Kuelbs \cite{K} has shown that every infinite-dimensional separable Banach space $\mathcal{B}$ can always be densely and continuously embedded in a separable Hilbert space $\mathcal{H}$. If $\mathcal{L}[\mathcal{B}]$ is the set bounded linear operators on $\mathcal{B}$, in this paper we prove the following: \begin{enumerate}\item For each $u \in \mathcal{B}$, there exists a semi-inner product $[\, \cdot, u]_z$ generated by bounded linear functional $u_z^* \in \mathcal{B}^*$ and a constant $c_u$ such that $u_z^*(v)=[v,u]_z= c_u(v , u)_\mathcal{H}$ for every $v \in \mathcal{B}$.\item If $\mathbb{A} \ne \mathcal{B}$ is closed, there exists $\mathbb{A}^\bot \subset \mathcal{B}$ disjoint, and $\mathcal{B} = \mathbb{A} \oplus \mathbb{A}^ \bot$.\item For $A \in \mcL[\mathcal{B}]$ there exists $A^* \in \mcL[\mathcal{B}]$ and $(A^*A)^*=A^*A$.\item $\mathcal{L}[\mathcal{B}] \subset \mathcal{L}[\mathcal{H}]$ as a continuous dense embedding.\item $\mathcal{B} \times \mathcal{B}^*$ has an Auerbach basis.\item Every compact operator on $\mathcal{B}$ is the limit of operators of finite rank without assuming that $\mathcal{B}$ has a Schauder basis.\item The Schatten class $\mathbb{S}_p [\mathcal{B}]$ exists for each $p \in [1, \iy]$, $\mathbb{S}_p [\mathcal{B}] \subset \mathbb{S}_p [\mathcal{H}]$ as a continuous dense embedding, $\bar{A} \in \mathbb{S}_p [\mathcal{H}]$ if and only if its restriction $A \in \mathbb{S}_p [\mathcal{B}]$, and ${\left\| {\bar A} \right\|_p^\mathcal{H}} = {\left\| A \right\|_p^\mathcal{B}}$. \end{enumerate}
WITHDRAWN: Do the Non-verbal Behaviors of Sprinters Before the Competition Affect Their Performance?
Authors: ERKAN Dilara , BAYRAKTAR Işık , BİÇER Ayşe Sibel 
Published: April 2023

Abstract
In athletics, the athlete is expected to get their concentration and control his negative emotions before the start. Body language of athletes can give important clues about their "real" internal states. On account of, it is important to analyze the nonverbal behaviors of the athletes before the start. In line with this importance, the aim of this study is to analyze the pre-start nonverbal behaviors of the athletes by coding them with the "Body Action Posture Coding System" and to examine the effect on the performance of the athletes. As a result, with this thesis study, it has been revealed that some nonverbal behaviors of the athletes, affect their performance. Nonverbal behaviors can give clues about the mental state of the athlete as well as give clues about the result of the competition has been opened to discussion with this study.
Presentation of Socialist Everyday Life in Transformation – Television Series “A Better Life”
Authors: Buljubašić Belma 
Published: April 2023

The paper analyzes the last Yugoslav television series A Better Life (Bolji život) the broadcast of which ended when the war had begun on the territory of the SFRY. More than thirty years after the end of the first broadcast of the series, it is still popular in Croatia, Bosnia and Herzegovina and Serbia, which is noticeable through numerous reruns, but also popularity on social networks, as well as numerous quotes from the series that are used in colloquial speech. The paper presents the content of the series and the main characters, as well as the most important secondary characters, briefly explains the then political and media situation in the different republics of the SFRY, which was completely uneven, which led to numerous antagonisms between nations, so in this context the series A Better Life was singled out as a series that was equally followed by citizens in all the republics, which was a phenomenon.
In the context of today, the series is analyzed through the prism of Yugonostalgia, and the content of the series is analyzed through several themes that appear in the series: everyday life and rituals, dealing with the consequences of the economic crisis, the conflict between rural and urban among the residents of Belgrade, i.e. the so-called old Belgraders and so-called newcomers.Certain stereotypes appeared in the series, primarily gender stereotypes, that is, the roles of men and women in society were repeatedly pointed out through the dialogues. Although at the time of filming of Better Life, inter-ethnic relations in SFRY were in crisis, the screenwriter skilfully avoided national stereotypes, although they appeared in several scenes, which is also included in the analysis of the series. Although the series depicts a politically turbulent time, nowadays it is viewed almost romantically, as a chance for a lost better life and missed opportunities.
Immunoinformatics and Reverse Vaccinology Driven Predication of a Multi-epitope
Vaccine against Borrelia burgdorferi and Validation through in silico Cloning and
Immune Simulation
Authors: Zheng Guojun , Hussain Zulfiqar , Hayat Chandni , Shahab Muhammad , Sikandar Ramin , Bibi Haleema , Kamil Atif , Liang Chaoqun 
Published: April 2023

Abstract:
Borrelia burgdorferi is regarded as an extremely dangerous bacteria causing infectious disease in humans, resulting in musculoskeletal pain, fatigue, fever and cardiac symptom. Because of all alarming concerns, no such prophylaxis setup has been available against Borrelia burgdorferi till now. In fact, vaccine construction using traditional methods is so expensive and time-consuming. Therefore, considering all concerns, we designed a multi-epitope-based vaccine design against Borrelia burgdorferi using in silico approaches.

Methods:
The present study utilized different computational methodologies, covering different ideas and elements in bioinformatics tools. The protein sequence of Borrelia burgdorferi was retrieved from the NCBI database. Different B and T cell epitopes were predicated using the IEDB tool. Efficient B and T cell epitopes were further assessed for vaccine construction using linkers AAY, EAAAK and GPGPG, respectively. Furthermore, the tertiary structure of constructed vaccine was predicated, and its interaction was determined with TLR9 using ClusPro software. In addition, further atomic level detail of docked complex and their immune response were further determined by MD simulation and C-ImmSim tool, respectively.

Results:
A protein with immunogenic potential and good vaccine properties (candidate) was identified based on high binding scores, low percentile rank, non-allergenicity and good immunological properties, which were further used to calculate epitopes. Additionally, molecular docking possesses strong interaction; seventeen H- bonds interactions were reported, such as THR101-GLU264, THR185-THR270, ARG 257-ASP210, ARG 257-ASP 210, ASP259-LYS 174, ASN263-GLU237, CYS 265- GLU 233, CYS 265-TYR 197, GLU267-THR202, GLN 270- THR202, TYR345-ASP 210, TYR345-THR 213, ARG 346-ASN209, SER350-GLU141, SER350-GLU141,
ASP 424-ARG220 and ARG426-THR216 with TLR-9. Finally, high expression was determined in E. coli (CAI = (0.9045), and GC content = (72%). Using the IMOD server, all-atom MD simulations of docked complex affirmed its significant stability. The outcomes of immune simulation indicate that both T and B cells represent a strong response to the vaccination component.

Conclusion:
This type of in-silico technique may precisely decrease valuable time and expenses in vaccine designing against Borrelia burgdorferi for experimental planning in laboratories. Currently, scientists frequently utilize bioinformatics approaches that speed up their vaccine-based lab work.
LincRNA-p21 Promotes Cellular Senescence by Down-regulating the
Wnt/β-catenin Pathway in MPP+-treated SH-SY5Y Cells
Authors: Zhu Jianyu , Chen Lingli 
Published: April 2023

Aim and Objective:
Long intergenic non-coding RNA-p21 (lincRNA-p21) plays a critical
role in various senescence-associated physiological and pathological conditions. We aimed to
explore the senescence-associated effects of lincRNA-p21 in 1-methyl-4-phenylpyridinium (MPP+)
treated neuroblastoma SH-SY5Y cell line as a therapeutic target.

Materials and Methods:
The RNA expression levels of lincRNA-p21, p53, p16, and telomere
length were examined with reverse transcription-quantitative polymerase chain reaction (RTqPCR).
The Telo TAGGG™ Telomerase PCR ELISA PLUS Kit was used to determine telomerase
activity. Cellular viability was evaluated with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay and lactate dehydrogenase (LDH) assay. Western blot was performed
to analyze β-catenin protein expression. Besides, oxidative stress was evaluated by Jaggregate-
forming delocalized lipophilic cation, 5,5',6,6'-tetrachloro-1,1',3,3'-
tetraethylbenzimidazolocarbocyanine++ + iodide (JC‑1) stain, fluorescence spectrophotometry,
colorimetric assay, and malondialdehyde (MDA) formation.

Results:
This research demonstrated that MPP+ caused a distinct increase in the expression of LincRNA-
p21 in SH-SY5Y cells. MPP+ induced cellular senescence with decreasing cellular proliferation
and viability, increasing expression levels of senescence-associated makers such as genes p53
and p16, accompanied by significantly decreasing telomere length and telomerase activity. At the
same time, these effects were abolished by silencing lincRNA-p21 with small interfering RNA
(siRNA). On the contrary, β-catenin silencing contributes to reversing anti-senescent effects caused
by lincRNA-p21 silencing. Moreover, modifying lincRNA-p21 exerted an anti-senescent influence
depending on decreasing oxidant stress.

Conclusion:
Our study showed that in the treatment of MPP+, lincRNA-p21 might serve a role in
the SH-SY5Y cell senescence by modulating the Wnt/β-catenin pathway, as well as increasing oxidant
stress. Thus, trying to target lincRNA-p21 may have important therapeutic and practical implications
for PD.
Interaction of Intestinal Microbiota with Medications
Authors: Liu Mengchen , Xu Jiaqiang , Jin Qianna , Li Yunqiao 
Published: April 2023

Introduction::
It is well known that the response to and metabolism of the drugs entering the human body
varies widely across individuals. One of the reasons is that such interpersonal differences may be related to gut microbes.
On one hand, drugs or xenobiotics entering the human body may affect the composition of the gut microbiome;
on the other hand, the gut microbiota may alter the absorption, distribution, metabolism and excretion (abbreviated
as ADME) process of drugs or xenobiotics vice versa. However, the majority of studies focused on the interaction
of general population cohorts with the gut microbiota, which is incompatible with the real clinic. For example,
the gut microbiota is closely associated with the progression and treatment of irritable bowel syndrome, a common
functional disorder of the gastrointestinal tract. Under the disease status, the composition of the gut microbiota is
altered affecting the pharmacokinetics, efficacy and toxicity of xenobiotics. Concerning irritable bowel syndrome, a
few studies reported that the xenobiotics administration process was gut microbial-mediated, while it also affected
drug efficacy and toxicity. Thus, the correlation between gut microbiota and xenobiotics administration, especially
the drugs administered, should be elucidated.

Method::
This review paper links differences between the gut microbiome and drug metabolism, which play a significant
role in the implications for medical therapy and drug development in irritable bowel syndrome indications.

Result::
The human intestinal microbiota permeates the ADME process of orally administered drugs and has the
potential to further modify the efficacy and toxicity of agents through the mediation of various enzymes, while at the
same time, medications could also alter the composition and function of the human intestinal microbiota.
Circumventing the Gastrointestinal Barrier for Oral Delivery of
Therapeutic Proteins and Peptides (PPTS): Current Trends and Future
Trajectories
Authors: Hashim Lily Ellysa , Sabri Akmal Hidayat , Mohamad Malihah Athirah , Anjani Qonita Kurnia , Mustaffa Mohd Faiz , Abdul Hamid Khuriah 
Published: April 2023

Abstract:
Therapeutic proteins and peptides (PPTs) have become one of the most important biological
molecules for the management of many common and complex diseases due to their high specificity and
high bioactivity. However, these biomolecules are mainly given by the hypodermic injection, which
often leads to poor patient compliance due to the invasive nature of this route of administration. The
oral route has been considered the most convenient and patient-friendly route for drug delivery relative
to hypodermic injections. Despite the ease and simplicity conferred by oral administration, this drug
delivery route suffers rapid peptide degradation in gastric fluid and low intestinal uptake. In order to
circumvent these issues, several strategies, such as enzyme inhibitors, permeation enhancers, chemical
modification, mucoadhesive and stimuli-responsive polymers, and specialised particulate formulation
have been developed. Such strategies are designed with the aim of protecting PPTs from the harsh gastrointestinal
environment as well as providing a strategy to enhance the uptake of the therapeutic across
the gastrointestinal tract. This review aims to provide an overview of the current development in enteral
drug delivery strategies for PPTs. The design of these drug delivery systems in overcoming physical
and chemical barriers along the gastrointestinal tract while improving oral bioavailability will be highlighted
and discussed.
Impact of COVID-19 Mandatory Lockdown Measures on Cognitive and Neuropsychiatric Symptoms in Persons with Alzheimer's Disease in Lima,
Peru
Authors: Custodio Nilton , Malaga Marco , Montesinos Rosa , Chambergo Diego , Baca Fiorella , Castro Sheila , Carbajal Juan C. , Herrera Eder , Lira David , Diaz Monica M. , Lanata Serggio 
Published: April 2023

Background:
Neuropsychiatric symptoms (NPS) in patients with Alzheimer’s disease (AD)
worsened during the COVID-19 lockdowns, but their progression thereafter is unknown. We present
the first longitudinal study tracking them before, during, and after restrictions.

Objectives:
To describe the effect of the COVID-19 mandatory lockdowns on Cognitive and Neuropsychiatric
symptoms in patients with Mild Cognitive Impairment (MCI) and Alzheimer’s Disease
(AD).

Methods:
Cohort of 48 patients with amnestic MCI and 38 with AD in Lima, Peru. They received
three rounds of cognitive (RUDAS, CDR, M@T), behavioral (NPI), and functional (ADCS-ADL) assessments.
We assessed the change in score means across the time points and for each domain of NPS
and tracked the changes in individual patients.

Results:
RUDAS declined 0.9 (SD 1.0) from baseline to lockdown and 0.7 (SD 1.0) after restrictions.
M@T declined 1.0 (SD 1.5) from baseline to lockdown and 1.4 (SD 2.0) after restrictions. CDR worsened
in 72 patients (83.72%) from baseline to post-lockdown. NPI worsened by 10 (SD 8.3) from
baseline to lockdown but improved by 4.8 (SD 6.4) after restrictions. Proportionally, 81.3% of all patients
had worsened NPS during the lockdowns, but only 10.7% saw an increase thereafter. Improvement
was statistically significant for specific NPS domains except hallucinations, delusions, and appetite
changes. Anxiety, irritability, apathy, and disinhibition returned to baseline levels.

Conclusion:
Following confinement, cognition continued to decline, but NPS demonstrated either
stability or improvement. This highlights the role modifiable risk factors may have on the progression
of NPS.