bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sosialisasi Pemanfaatan Situs Sejarah sebagai Laboratorium Lapangan Jurusan Ilmu Sejarah
Authors: Mukadas Aswati , Aslim Aslim , Burhan Faika , Sarman Sarman , Hasan Hasni 
Published: January 2025

Pelaksanaan kegiatan ini bertujuan memberikan sosialisasi pemanfaatan situs sejarah sebagai laboratorium lapangan sebagai salah satu upaya menjaga dan melestarikan situs sejarah. Metode yang dilakukan adalah sosialisasi pemberian pemahaman pada masyarakat, serta tanya jawab terkait kondisi situs sejarah yang ada. Hasil dari pengabdian ini antara lain memberikan edukasi pemahaman kepada masyarakat untuk menjaga situs sejarah dan bekerjasama dalam upaya pelestarian situs sejarah melalui kegiatan laboratorium lapangan yang terdapat di Kelurahan Kampung Salo Kecamatan Kendari Kota Kendari. Kegiatan sosialisasi dilaksanakan pada bulan Oktober 2022 di Kelurahan Kampung Salo Kecamatan Kendari Kota Kendari.
PELATIHAN PENULISAN ARTIKEL ILMIAH DAN PENGENALAN ONLINE JOURNAL SYSTEM (OJS) PADA GURU BAHASA MAN I KENDARI
Authors: Rasiah Rasiah , Sofian Nur Israfyan , Gultom Eva Solina , Azi Rahmawati , Ulya Afriani , Bilu La Bilu , Samarudin Samar , Aji Adin Purnomo 
Published: January 2025

Pengabdian ini bertujuan untuk mensosialisasikan publikasi ilmiah melalui platform Online Journal System (OJS) pada guru-guru bahasa MAN 1 Kendari. Analisis situasi mitra (MAN 1 Kendari) menunjukkan bahwa guru-guru bahasa kurang memiliki motivasi untuk publikasi karya yang juga menunjang tidak hanya dalam peningkatan kompetensi mereka, tetapi juga menunjang pengusulan pangkat/golongan. Kurangnya motivasi tersebut diperkuat dengan adanya pemutakhiran sistem operasional jurnal ilmiah yang mulai mengoperasikan sistem jaringan internet atau OJS sehingga publikasi jurnal menjadi paperless. Tim Pengabdian kemudian merancang kegiatan pelatihan penulisan artikel ilmiah dan pengenalan OJS pada guru-guru bahasa MAN 1 Kendari. Dalam kegiatan tersebut, Tim pengabdian menghadirkan narasumber yang membahas publikasi ilmiah dan sistem pengelolaan OJS yang terdiri dari tim Dosen dari Universitas Halu Oleo. Kegiatan tersebut di laksanakan pada tanggal 12 Agustus 2023 bertempat di ruang Multimedia MAN 1 Kendari. Luaran kegiatan PKM ini diharapkan terjadinya peningkatan keberdayaan mitra dalam penulisan artikel ilmiah dan publikasi di jurnal ilmiah.
hsa-miR-548d-3p: a potential microRNA to target nucleocapsid and/or capsid genes in multiple members of the Flaviviridae family
Authors: Cayatineto H. W. , Hakim S. T. 
Published: January 2025

IntroductionFlaviviridae comprise a group of enveloped, positive-stranded RNA viruses that are mainly transmitted through either mosquitoes or tick bites and/or contaminated blood, blood products, or other body secretions. These viruses cause diseases ranging from mild to severe and are considered important human pathogens. MicroRNAs (miRNAs) are non-coding molecules involved in growth, development, cell proliferation, protein synthesis, apoptosis, and pathogenesis. These small molecules are even being used as gene suppressors in antiviral therapeutics, inhibiting viral replication. In the current study, we used bioinformatic tools to predict a possible miRNA sequence that could be complementary to the nucleocapsid (NP) and/or capsid (CP) gene of the Flaviviridae family and provide an inhibitory solution.MethodsBioinformatics is a field of science that includes tremendous computational analysis, logarithms, and sequence alignments. To predict the right alignments between miRNA and viral mRNA genomes, we used computational databases such as miRBase, NCBI, and Basic Alignment Search Tool–nucleotides (BLAST-n).ResultsOf the 2,600 mature miRNAs, hsa-miR-548d-3p revealed complementary sequences with the flavivirus capsid gene and bovine viral diarrhea virus (BVDV) capsid gene and was selected as a possible candidate to inhibit flaviviruses.ConclusionAlthough more detailed in vitro and in vivo studies are required to test the possible inhibitory effects of hsa-miR-548d-3p against flaviviruses, this computational study may be the first step to study further, developing a novel therapeutic for lethal viruses within the Flaviviridae family using suggested candidate miRNAs.
A computational chemistry-based approach to optimizing PD-1/PD-L1 inhibitors
Authors: Zhai Meijuan , Ji Shiliang , Hu Haoran , Wu Yongjie , Shi Yi , Zhu Ruifang , Jiang Yiguo , Yang Yang 
Published: January 2025

IntroductionTo design effective small molecule inhibitors targeting the immune checkpoint PD-1/PD-L1 and to explore their inhibitory activity.MethodsIn this paper, a total of 69 PD-1/PD-L1 inhibitors with the same backbone were searched through opendatabases, and their docking mechanism with PD-L1 protein was investigatedby molecular docking method, and the active conformation of the inhibitors was explored. The biological activity of the four newly designed inhibitors was also evaluated using ELISA.ResultsThe most active molecule 58 in the dataset formed six hydrogen bonds with Phe67, Val55, Ile116 and Tyr123, while the second most active molecule 34 formed five hydrogen bonds with Phe67 and Ala121, both of which formed π-π stacking interactions with Tyr56. The analysis of the inhibitor docking results determined that the residues Tyr123, Gln66, Thr20, Met115, Asp122 and Ile116 had the greatest influence on the active conformation of the inhibitor. ELISA assays suggested that the four novel inhibitors designed had high inhibition rates, with the inhibition rate of compound N2 being as high as 68.53%.DiscussionIn this paper, we have designed and synthesized various PD-1/PDL1 inhibitors, which provide a basis for drug discovery targeting the PD-1/PDL1 signaling pathway.
Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections
Authors: Zhou Pengjun , Shi Xing , Xia Jinquan , Wang Yifei , Dong Shaowei 
Published: January 2025

BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) poses a significant challenge in clinical environments due to its resistance to standard antibiotics. Staphylococcal Protein A (SpA), a crucial virulence factor of MRSA, undermines host immune responses, making it an attractive target for vaccine development. This study aimed to identify potential epitopes within SpA that could elicit robust immune responses, ultimately contributing to the combat against multidrug-resistant (MDR) MRSA.MethodsThe SpA protein sequence was retrieved from the UniProt database, with various bioinformatics tools employed for epitope prediction. T-cell epitopes were identified using the Tepitool server, focusing on high-affinity interactions with prevalent human leukocyte antigens (HLAs). B-cell epitopes were predicted using the BepiPred tool. Predicted epitopes underwent docking studies with HLA molecules to evaluate binding properties. In-silico analyses confirmed the antigenicity, promiscuity, and non-glycosylated nature of the selected epitopes.ResultsSeveral T and B cell epitopes within SpA were identified, showcasing high binding affinities and extensive population coverage. A multi-epitope vaccine construct, linked by synthetic linkers and an adjuvant, was modelled, refined, and validated through various bioinformatics assessments. The vaccine candidate was subsequently docked with Toll-like receptor 4 (TLR-4) to evaluate its potential for immunogenicity.ConclusionThis study lays the groundwork for developing epitope-based vaccines targeting SpA in MRSA, identifying promising candidates for experimental validation and contributing to innovative immunotherapeutic strategies against MRSA infections.
Optimal timing of pharmacoinvasive strategy and its impact on clinical and economic outcomes in patients with ST-elevation myocardial infarction: a real-world perspective
Authors: Stephanus Andrea Dias , Santos Alice Pacheco , Rodrigues da Cunha Ana Carolina Machado , Rocha Ana Carolina Augusto , Meireles Amanda de Amorim , de Oliveira Mariana Guimarães Souza , Matsunaga Pietra Arissa Coelho , Soares Alexandre Anderson de Sousa Munhoz , Nogueira Ana Claudia Cavalcante , Guimarães Adriana de J. B. de Almeida , Alexim Gustavo de Almeida , Campos-Staffico Alessandra M. , de Carvalho Luiz Sergio Fernandes 
Published: January 2025

BackgroundThe pharmacoinvasive (PhI) strategy is the standard-of-care for ST-elevation myocardial infarction (STEMI) patients when primary percutaneous coronary intervention (pPCI) is unfeasible. Optimal timing for post-fibrinolytic PCI (lysis-PCI) remains elusive. Therefore, this study aimed to assess the clinical and economic impacts of early vs. delayed lysis-PCI in patients with STEMI.MethodsThis retrospective cohort study included 1,043 STEMI patients classified by lysis-PCI timing. The primary outcome was in-hospital major adverse cardiovascular events (4p-MACE), with secondary outcomes such as 3p-MACE, in-hospital mortality, and costs. Multivariable logistic regression models were used to assess the association between lysis-PCI timing and outcomes. Cost analyses were conducted from the perspective of Brazilian public healthcare system, with values converted to international dollars (Int$) for broader applicability.ResultsEvery 4-h delay in lysis-PCI was associated with a 44% reduction in in-hospital mortality [OR = 0.560 (95% CI: 0.381–0.775); p = 0.001] and a 13% decrease in 4p-MACE [OR = 0.877 (95% CI: 0.811–0.948); p = 0.001]. Additionally, 4-h delay in lysis-PCI was also associated with a significant reduction in in-hospital costs (Int$916.20 ± 99) and disease-induced years of productivity lost (β = −41.79 ± 151 years; p = 0.001). These significant trends remained consistent even after adjusting for confounders and applying propensity score matching. Older adults (aged ≥80) experienced an increase in 3p-MACE with earlier lysis-PCI.ConclusionDelaying lysis-PCI was found to be associated with reduced in-hospital cardiovascular adverse events and lower costs, particularly among older adults. Further research should develop evidence-based lysis-PCI protocols that optimize both clinical outcomes and cost-effectiveness.
Cardiac resynchronization therapy via left bundle branch pacing in heart failure with complete left bundle branch block: is the defibrillator needed?
Authors: Yang Dandan , Ma Qunchao , Zhu Hong , Wang Lihua , Xiang Meixiang , Wang Jian’an , Pan Xiaohong 
Published: January 2025

AimsThis retrospective cohort study aimed to investigate the efficacy of dual-chamber left Bundle branch pacing (LBBP) as an alternative therapy for heart failure patients with complete left bundle branch block (CLBBB) and indications for defibrillator with cardiac resynchronization therapy (CRT-D).Methods34 patients met inclusion criteria were enrolled in the study. These criteria included a left ventricular ejection fraction (LVEF) of lower than 35%, a New York Heart Association functional class of II–IV, CLBBB meeting Strauss's criteria, intraventricular dyssynchrony, and confirmed correction of CLBBB during LBBP. Patients with ischemic cardiomyopathy, left ventricular noncompaction, significant late gadolinium enhancement (LGE) on cardiac magnetic resonance imaging (CMR), and indications for an implantable cardioverter-defibrillator (ICD) as secondary prevention were excluded.ResultsPost-LBBP, the LVEF improved from 31.1 ± 4.0% to 61.0 ± 6.0% (P &lt; 0.001). All patients exhibited a super-response to LBBP cardiac resynchronization therapy, achieving complete improvement in cardiac function with a LVEF exceeding 50%. Septal-to-posterior wall motion delay (SPWMD) and systolic dyssynchrony index (SDI) were indicators of intraventricular synchrony, SPWMD decreased from 271.4 ± 76.4 ms to 42.2 ± 22.9 ms (P &lt; 0.001), and SDI decreased from 12.5 ± 5.3% to 1.9 ± 1.0% after implantation (P &lt; 0.001).ConclusionsHeart failure patients meeting the following criteria may be considered for dual-chamber pacing as an alternative to CRT-D, potentially avoiding the need for ICD implantation: (1) CLBBB meeting Strauss's criteria, (2) presence of intraventricular dyssynchrony on echocardiogram, (3) exclusion of secondary prevention ICD indications, (4) absence of evident LGE on CMR, and (5) successful correction of CLBBB during LBBP.
A 7,000 years trajectory of socio-ecosystems in the montane belt of the northern French Alps
Authors: Julien Andréa , Messager Erwan , Doyen Elise , Etienne David , Marquer Laurent , Giguet-Covex Charline 
Published: January 2025

IntroductionThis study, conducted on the Plateau des Glières, in the northern French Alps, investigates the evolution of vegetation composition over the past 7000 years. This site, within a montane ecosystem, offers a new opportunity to understand the human and landscape interactions under the influence of climate. To study these interactions as a whole and by integrating their complexity, we used two conceptual frameworks: the agroecosystem and the trajectory.MethodsThe analyses conducted are based on a detailed analysis of pollen, NPP, macro-charcoal, and geochemical data from peat cores.ResultsThe vegetation history of the Plateau des Glières is dominated by forest, due to its location within the montane belt (1,435 m). However, the vegetation composition trajectory of the Glières reveals three regimes: two are characterized by an evolutionary trend of the system, and between them, the a third regime is characterized by a “dynamic steady state” of the system. The first regime, corresponding to the Neolithic period, is initially characterized by a closed forested environment with a progressive trend toward a more open landscape due to the first agro-pastoral activities. The second regime is recorded from the very end of the Neolithic until the High Roman Empire. It corresponds to a relatively stationary system, characterized by the progressive development of pastoral activities leading to low impacts on the landscape. This long period (4300 to 1800 cal. BP) is characterized by the sustainability of the agroecosystem that developed. After this long pseudo-stable phase, a tipping point in the vegetation composition trajectory is highlighted. The third regime, spanning from the Migration Period to the present day, is the result of the intensification and diversification of agro-pastoral activities (pastoralism, cereal crops, and fruit trees). The landscape that we can see today in the Plateau des Glières is the legacy of this trajectory characterizing the interactions between ecosystems and human societies, i.e., the socio-ecosystem.DiscussionWhile the agroecosystem trajectory reconstructed on the Plateau des Glières is rather consistent with the altitudinal model of anthropization previously proposed for this region, the vegetation response to the activities is unusual because it shows a long phase of ecosystem stability despite the relatively high human pressure.
Loading of Ce and n co-doped TiO2 composites onto modified shell powder synergism of adsorption and photocatalysis phytic acid removal: performance and mechanism
Authors: Zhang Wei , Wang Zheng , Shu Jinkai , Wang Aihe , Lin Hai , Yan Xuchao 
Published: January 2025

Phytic acid was investigated as an organophosphorus pollutant. Titanium dioxide (TiO2) doped with Ce and N was prepared using the sol-gel method and loaded onto a modified shell power to produce modified shell powder/Ce-N-TiO2 (Msp/CeNT). The combined effects of adsorption and photocatalysis on phytic acid were explored. The actual phytic acid degradation rate with the composite photocatalyst was higher than the sum of the adsorption removal rate of phytic acid using the modified shell powder and the photocatalytic degradation removal rate of phytic acid using the Ce-N-TiO2 photocatalyst. Msp/CeNT synergistically affected adsorption and photocatalytic degradation. The effects of different factors, such as reaction temperature, catalyst dosage, initial pH, stirring speed, and light intensity, on the combined effect were investigated. The results showed that the synergistic effect increases with the increase of light intensity. Increasing the reaction temperature, catalyst dosage, initial pH, and stirring speed first increased and then decreased the synergistic effect of the composite photocatalysts. Phytic acid (69.54%) was degraded within 4 h when the temperature, pH, catalyst dosage, stirring speed, and light intensity were 25 °C, 5, 1 g/L, 300 rpm, and 500 W, respectively. We investigated and prepared a composite photocatalytic material, developing a new theoretical method for degrading organophosphorus dissolved in water and providing a basis for treating lake eutrophication as a practical application.
Impact of SARS-CoV-2 vaccination and of seasonal variations on the innate immune inflammatory response
Authors: Jarras Hend , Blais Isalie , Goyer Benjamin , Bazié Wilfried W. , Rabezanahary Henintsoa , Thériault Mathieu , Santerre Kim , Langlois Marc-André , Masson Jean-François , Pelletier Joelle N. , Brousseau Nicholas , Boudreau Denis , Trottier Sylvie , Baz Mariana , Gilbert Caroline 
Published: January 2025

IntroductionThe innate immune response is an important first checkpoint in the evolution of an infection. Although adaptive immunity is generally considered the immune component that retains antigenic memory, innate immune responses can also be affected by previous stimulations. This study evaluated the impact of vaccination on innate cell activation by TLR7/8 agonist R848, as well as seasonal variations.MethodsTo this end, blood samples from a cohort of 304 food and retail workers from the Quebec City region were collected during three visits at 12-week intervals. Peripheral blood mononuclear cells and polymorphonuclear neutrophils were isolated during the first and third visits and were stimulated with R848 to assess the innate immune response.ResultsOur results show that IL-8 production after stimulation decreased after vaccination. In addition, the IL-8 response was significantly different depending on the season when the visit occurred, for both COVID-19 vaccinated and unvaccinated individuals.DiscussionThis study highlights that innate immune responses can be affected by SARS-CoV-2 vaccination and fluctuate seasonally.
Meta-analysis of retinal transcriptome profiling studies in animal models of myopia
Authors: Palumaa Teele , Balamurugan Shruti , Pardue Machelle T. 
Published: January 2025

ObjectiveMyopia prevalence is increasing at alarming rates, yet the underlying mechanistic causes are not understood. Several studies have employed experimental animal models of myopia and transcriptome profiling to identify genes and pathways contributing to myopia. In this study, we determined the retinal transcriptome changes in response to form deprivation in mouse retinas. We then conducted a transcriptome meta-analysis incorporating all publicly available datasets and analyzed how the results related to the genes associated with refractive errors in human genome-wide association studies (GWAS).MethodsForm deprivation was induced in three male C57BL6/J mice from postnatal day 28 (P28) to P42. Retinal gene expression was analyzed with RNA sequencing, followed by differential gene expression analysis with DESeq2 and identification of associated pathways with the Kyoto Encyclopedia of Genes and Genomes (KEGG). A systematic search identified four similar retinal transcriptomics datasets in response to experimental myopia using chicks or mice. The five studies underwent transcriptome meta-analyses to determine retinal gene expression changes and associated pathways. The results were compared with genes associated with human myopia.ResultsDifferential gene expression analysis of form-deprived mouse retinas revealed 235 significantly altered transcripts, implicating the BMP2 signaling pathway and circadian rhythms, among others. Transcriptome-wide meta-analyses of experimental myopia datasets found 427 differentially expressed genes in the mouse model and 1,110 in the chick model, with limited gene overlap between species. Pathway analysis of these two gene sets implicated TGF-beta signaling and circadian rhythm pathways in both mouse and chick retinas. Some pathways associated only with mouse retinal changes included dopamine signaling and HIF-1 signaling pathway, whereas glucagon signaling was only associated with gene changes in chick retinas. The follistatin gene changed in both mouse and chick retinas and has also been implicated in human myopia. TGF-beta signaling pathway and circadian entrainment processes were associated with myopia in mice, chicks, and humans.ConclusionThis study highlights the power of combining datasets to enhance statistical power and identify robust gene expression changes across different experimental animal models and conditions. The data supports other experimental evidence that TGF-beta signaling pathway and circadian rhythms are involved in myopic eye growth.
Salivary alpha-amylase activity and its association with early childhood caries and rampant caries experience: a cross-sectional study
Authors: Padmanabhan Vivek , Islam Md Sofiqul , Rahman Muhammed Mustahsen , Goud Manjunatha B. K. , Alshehhi Latifa Majed Sulaiman Allay , Hamed Hebah Mushref Ahmad , Haridas Sheela , Sharma Dileep 
Published: January 2025

AimsThis study aims to evaluate salivary alpha-amylase levels in children diagnosed with Early Childhood Caries (ECC) and Rampant Caries (RC) and compare them to levels in children without ECC or RC. It also examines the relationship between salivary alpha-amylase levels and increased caries activity in the children with ECC or RC.Materials and methodsA cross-sectional study was conducted at RAK College of Dental Sciences (RAKCODS) with 100 children aged 3–12 years. Participants were divided into ECC and RC groups, each consisting of study and control groups. Salivary alpha-amylase levels were measured using Enzyme-Linked Immunosorbent Assay (ELISA) kits. Statistical analyses, including the Wilcoxon Signed Rank test and Pearson’s correlation coefficient, were performed using SPSS version 29 to compare salivary alpha-amylase levels between groups and examine correlation with severity of dental caries.ResultsThe study found that children with ECC and RC had significantly higher mean salivary alpha-amylase levels (16.046 U/mL and 20.62 U/mL, respectively) compared to control groups (5.09 U/mL and 12.70 U/mL). The differences were statistically significant (p &lt; 0.0001). Pearson’s correlation coefficients indicated a strong positive correlation between salivary alpha-amylase levels and severity of dental caries in both ECC (r = 0.9891, p &lt; 0.0001) and RC (r = 0.9142, p &lt; 0.0001) groups.ConclusionThe study concludes that elevated salivary alpha-amylase levels, which are statistically significant, are observed in children with ECC and RC. Additionally, it was found that salivary alpha-amylase levels increased with the number of carious lesions. These findings suggest that salivary alpha-amylase could serve as a valuable biomarker for assessing caries risk and guiding preventive strategies.