bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unique Profile of Inflammation and Immune Activation in Pregnant People With HIV in the United States
Authors: Shiau Stephanie , Jacobson Denise L , Huo Yanling , Kacanek Deborah , Yee Lynn M , Williams David B , Haddad Lisa B , Serghides Lena , Powis Kathleen , Sperling Rhoda S , Williams Paige L , Jao Jennifer ,   for the Pediatric HIV/AIDS Cohort Study, Chadwick Ellen , Sanders Margaret Ann , Malee Kathleen , Paul Mary , Eser-Jose Ruth , McMullen-Jackson Chivon , Harris Lynnette , Purswani Murli , Baig Mahoobullah Mirza , Villegas Alma , Alvarado Marvin , Robinson Lisa-Gaye , Cooley Jawara Dia , Blood James , Garvie Patricia , Borkowsky William , Deygoo Nagamah Sandra , Lewis Jennifer , Dieudonne Arry , Bettica Linda , Johnson Juliette , Surowiec Karen , Knapp Katherine , Russell-Bell Jamie , Wilkins Megan , Love Stephanie , Rosario Nicolas , Angeli-Nieves Lourdes , Olivera Vivian , Kohlhoff Stephan , Dennie Ava , Kaye Jean , Wallier Jenny , Craig Karen , Silio Margarita , Sirois Patricia , Hutto Cecelia , Hickman Paige , Huldtquist Julie , Marullo Dan , Spector Stephen A , Figueroa Veronica , Loughran Megan , Nichols Sharon , McFarland Elizabeth , Kwon Christine , Glenny Carrie , Englund Jennifer , Rathore Mobeen , Mahmoudi Saniyyah , El-Hassan Sarah , Tejan Jamilah , Hayani Karen , Richardson Lourdes , Smith Renee , Miller Alina , Scott Gwendolyn , Garcia Gustavo Gil , Fernandez Gabriel , Cuadra Anai , Frederick Toni , Davtyan Mariam , Morales-Avendano Guadalupe , Rodriguez Zoe M , Torres Lizmarie , Scalley Nydia 
Published: January 2023

Abstract

Background
Little is known about inflammation/immune activation during pregnancy in people with HIV (PWH) and growth in their children who are HIV-exposed and uninfected (CHEU).

Methods
Using data from the Pediatric HIV/AIDS Cohort Study and an HIV-seronegative comparison group, we assessed associations of (1) HIV status, mode of HIV acquisition (perinatally vs nonperinatally acquired), and type of antiretroviral therapy (ART) with inflammation/immune activation in pregnancy; and (2) inflammation/immune activation in pregnancy with growth of CHEU at 12 months. Interleukin 6 (IL-6), high-sensitivity C-reactive protein (hs-CRP), soluble(s) TNF-α receptor 1 and 2 (sTNFR1, sTNFR2), sCD14, and sCD163 were measured between 13 and 27 weeks' gestation. Linear regression models were fit to estimate differences between groups for each log-transformed biomarker, adjusted for confounders.

Results
Pregnant PWH (188 total, 39 perinatally acquired, 149 nonperinatally acquired) and 76 HIV-seronegative persons were included. PWH had higher IL-6, sTNFR1, sCD14, and sCD163 and lower sTNFR2 compared to HIV-seronegative persons in adjusted models. Among PWH, sCD163 was higher in those with perinatally versus nonperinatally acquired HIV and on PI-based versus INSTI-based ART. Higher maternal concentrations of IL-6, sTNFR2, and hs-CRP were associated with poorer growth at 12 months.

Conclusions
Maternal HIV status is associated with a distinct profile of inflammation/immune activation during pregnancy, which may influence child growth.
HACCP plan for microbiological hazards associated with fermented crab,Episesarma mederiH. Milne Edwards 1853
Authors: Boonprab Kangsadan , Donrung Nowwapan 
Published: January 2023

AbstractAimsTo develop a model HACCP plan related to the microbiological hazards for the traditional fermented crab.Methods and ResultsThe microbiological and chemical characteristics of commercial products were surveyed. Microbiological hazard analysis was performed for raw materials and during processing. Critical control points (CCPs) were determined using a decision tree, with CCP1 as saturated salt preparation and CCP2 as fermentation. The critical limit (CL) of CCP1 was at 100°C for 20 min applied to brining and of CCP2 was at 25% NaCl for the brine applied to fermented crab. Isolated microbial hazards and type strains were used for the validation of the CLs. Monitoring and verification of the proposed HACCP plan were carried out, and an effective HACCP plan was established.ConclusionsThe HACCP plan promoted the safe consumption of fermented crab with the provided CCPs at the saturated salt preparation and fermentation steps. The effective CLs to ensure microbiological hazards as safe at the CCPs provide the best support for an effective plan. The hazards were reduced significantly after the HACCP plan had been applied.
A novel Staphylococcus phage, vB_Sau-RP15, and its application in contaminated milk
Authors: Imklin Napakhwan , Chaengphaniad Phiraphat , Šimoliūnas Eugenijus , Nasanit Rujikan 
Published: January 2023

Abstract
The vB_Sau-RP15 phage, selected for its potential use as a phage treatment in milk, was isolated from raw milk using Staphylococcusaureus NP01 as the host. The host range test revealed that the phage was able to lyse 12 strains of Staph. aureus from raw milk. This phage was stable at 4–37°C and pH 6–9 for at least 1 h. The adsorption rate was ~78% within the first 3 min. A low frequency of phage-insensitive mutant induction (4.6 × 10−6) was observed. Genomic analyses revealed that the vB_Sau-RP15 represented a novel species in the genus Silviavirus. Even though no virulence or antibiotic resistance genes were detected, the phage genome carried lysogenic-associated genes. Phage treatments (108 PFU per ml) in pasteurized milk contaminated with low (104 CFU per ml) and high (107 CFU per ml) concentrations of Staph. aureus confirmed the proficiency of the phage in the diminishing of the number of bacterial cells at 4°C and ambient temperature. A Staphylococcus phage, vB_Sau-RP15, could be a promising agent for controlling Staph. aureus contamination in milk.
Evaluating the efficacy of hydrogen peroxide vapor against a retroviral vector in a gene therapy manufacturing facility
Authors: Law Roshanak , Ferrige Georgina , Perrett Kathryn , Langley Christopher , Chewins John 
Published: January 2023

Abstract
An evaluation of the efficacy of 35% hydrogen peroxide vapor (HPV) against a novel Simian Immunodeficiency retrovirus (SIV) was conducted in a 624m3 GMP grade C manufacturing facility. SIV biological indicators, with an average titre of 7.11×108 TU/mL [equivalent to 1.3×108 Reverse transcriptase (RT) nanounits when analysed by F-PERT], were produced on-site using virus presented within an anticipated worst-case support matrix based on the manufacturers commercially confidential virus support media. SIV biological indicators were distributed around the manufacturing facility, including locations identified as key contamination control points by the manufacturer, and exposed to a HPV cycle. Commercially available biological indicators of 6 log10 Geobacillus stearothermophilus were co-located with the SIV Biological Indicator (BIs). HPV was found to be efficacious in eliminating the SIV and commercial G. stearothermophilus BIs and offers a simple and robust method for biodecontamination of gene therapy facilities.
Microencapsulation of Lacticaseibacillus rhamnosus GG and L. plantarum 299 V by reverse spherification: a promising method to improve the survival of probiotics
Authors: Khajehie Nastaran , Niakousari Mehrdad , Eskandari Mohammad Hadi , Majdinasab Marjan , Shekarforoush Seyed Shahram 
Published: January 2023

Abstract
This research reports the first application of the reverse spherification (RVS) method for encapsulation of two probiotics (Lacticaseibacillus rhamnosus GG and L. plantarum 299 V) compared to the basic spherification (BS). These probiotics were encapsulated in different solutions encompassing various contents of alginate, gelatin, and gellan gum. The RVS bead diameters was about 1.5 times bigger and hardness was 70%–80% lower than BS samples. As determined by Raman spectral mapping, the RVS beads had two calcium alginate walls but the BS beads had only one. The inner wall of the RVS beads was more than three times thicker than outer wall. The encapsulation yields of gelatin/gellan gum and gelatin beads prepared by both methods were &gt;1.5% alginate beads. All the RVS-prepared beads were resistant to stomach acid and showed no significant reduction in the intestine. Furthermore, the incorporation of gelatin and gellan gum into alginate led to higher cell protection. For 1.5% alginate beads, &lt;67% survival was achieved after acid exposure but in others, &gt;77% survival was observed; RVS beads were about 1 log above than BS ones. The proposed novel microencapsulation method efficiently increased the viability of probiotic bacteria compared to the conventional approaches.
Characterization of selected species of Pichia and Candida for their growth capacity in apple and grape must and their biofilm parameters
Authors: Lorenzini Marilinda , Cappello Maria Stella , Andreolli Marco , Zapparoli Giacomo 
Published: January 2023

Abstract
Pichia and Candida species include biofilm-forming yeasts able to spoil foods and beverages. Strains belonging to 10 Pichia and Candida species isolated from apples, grape musts, and wines were analysed. They were subjected to molecular typing and characterized for their ability to grow and ferment must for cider and wine production, and for their biofilm properties. All strains grew similarly in apple and grape must. Glucose-fermenting strains displayed differentiated fermentation performances. Great variation in SO2 and ethanol sensitivity was observed among the strains. Pichia manshurica strains showed high tolerance to both molecules. Eleven and five surface-spreading biofilm (MAT) phenotypes were identified in solid and liquid media, respectively. Strains produced biofilms with variable thicknesses and widths in culture tubes. Cell adherence and aqueous-hydrocarbon biphasic hydrophobicity assays were carried out. Some Pichia manshurica and P. membranifaciens strains exhibited a high capacity to form a thick biofilm and had high cell adherence and hydrophobicity values. These strains could be more likely to colonize the internal surfaces of tanks. This study evidenced that some Pichia and Candida strains can proliferate during apple and grape must fermentation and may be detrimental the beverage quality, due to their specific biofilm properties.
A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
Authors: de la Herrán Roberto , Hermida Miguel , Rubiolo Juan Andres , Gómez‐Garrido Jèssica , Cruz Fernando , Robles Francisca , Navajas‐Pérez Rafael , Blanco Andres , Villamayor Paula Rodriguez , Torres Dorinda , Sánchez‐Quinteiro Pablo , Ramirez Daniel , Rodríguez Maria Esther , Arias‐Pérez Alberto , Cross Ismael , Duncan Neil , Martínez‐Peña Teresa , Riaza Ana , Millán Adrian , De Rosa M. Cristina , Pirolli Davide , Gut Marta , Bouza Carmen , Robledo Diego , Rebordinos Laureana , Alioto Tyler , Ruíz‐Rejón Carmelo , Martínez Paulino 
Published: January 2023

AbstractSex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long‐ and short‐read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC‐FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post‐fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y‐linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X‐linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.
A compilation answering 50 questions on monkeypox virus and the current monkeypox outbreak
Authors: Cabanillas Beatriz , Murdaca Giuseppe , Guemari Amir , Torres Maria Jose , Azkur Ahmet Kursat , Aksoy Emel , Vitte Joana , de las Vecillas Leticia , Giovannini Mattia , Fernández‐Santamaria Ruben , Castagnoli Riccardo , Orsi Andrea , Amato Rosa , Giberti Irene , Català Alba , Ambrozej Dominika , Schaub Bianca , Tramper‐Stranders Gerdien A. , Novak Natalija , Nadeau Kari C. , Agache Ioana , Akdis Mubeccel , Akdis Cezmi A. 
Published: January 2023

AbstractThe current monkeypox disease (MPX) outbreak constitutes a new threat and challenge for our society. With more than 55,000 confirmed cases in 103 countries, World Health Organization declared the ongoing MPX outbreak a Public Health Emergency of International Concern (PHEIC) on July 23, 2022. The current MPX outbreak is the largest, most widespread, and most serious since the diagnosis of the first case of MPX in 1970 in the Democratic Republic of the Congo (DRC), a country where MPX is an endemic disease. Throughout history, there have only been sporadic and self‐limiting outbreaks of MPX outside Africa, with a total of 58 cases described from 2003 to 2021. This figure contrasts with the current outbreak of 2022, in which more than 55,000 cases have been confirmed in just 4 months. MPX is, in most cases, self‐limiting; however, severe clinical manifestations and complications have been reported. Complications are usually related to the extent of virus exposure and patient health status, generally affecting children, pregnant women, and immunocompromised patients. The expansive nature of the current outbreak leaves many questions that the scientific community should investigate and answer in order to understand this phenomenon better and prevent new threats in the future. In this review, 50 questions regarding monkeypox virus (MPXV) and the current MPX outbreak were answered in order to provide the most updated scientific information and to explore the potential causes and consequences of this new health threat.
Perinatal risk factors for developmental coordination disorder in children born extremely preterm
Authors: Bolk Jenny , Källén Karin , Farooqi Aijaz , Hafström Maria , Fellman Vineta , Åden Ulrika , Serenius Fredrik 
Published: January 2023

AbstractAimChildren born extremely preterm frequently have developmental coordination disorder (DCD). We aimed to evaluate perinatal risk factors for DCD.MethodsSwedish national cohort study including 226 children born before 27 gestational weeks without major neurodevelopmental disabilities at 6.5 years. Outcome was DCD, defined as ≤5th percentile on the Movement Assessment Battery for Children‐Second Edition. Perinatal risk factors were evaluated using multivariable logistic regression.ResultsDCD was present in 84/226 (37.2%) children. Of the risk factors known at 40 weeks gestation, independent and significant risk factors for DCD were: mother's age at delivery (odds ratio [OR] 1.73, 95% confidence interval [CI] 1.07–2.80); pre‐eclampsia (2.79, 1.14–6.80); mother born in a non‐Nordic country (2.23, 1.00–4.99); gestational age per week increase (0.70, 0.50–0.99) and retinopathy of prematurity (2.48, 1.26–4.87). Of factors known at discharge, postnatal steroids exposure (2.24, 1.13–4.46) and mechanical ventilation (1.76, 1.06–2.09) were independent risk factors when added to the model in separate analyses.ConclusionThe risk of DCD in children born extremely preterm was multifactorial and associated with gestational age largely mediated by ROP, maternal factors, pre‐eclampsia, administration of postnatal steroids and mechanical ventilation. These risk factors are common among children born extremely preterm, contributing to their high risk of DCD.
Characterising nationwide reasons for unplanned hospital readmission after colorectal cancer surgery
Authors: Xu William , Wells Cameron I. , McGuinness Matthew , Varghese Chris , Keane Celia , Liu Chen , O'Grady Gregory , Bissett Ian P. , Harmston Christopher 
Published: January 2023

AbstractBackgroundReadmissions after colorectal cancer surgery are common, despite advancements in surgical care, and have a significant impact on both individual patients and overall healthcare costs. The aim of this study was to determine the 30‐and 90 days readmission rate after colorectal cancer surgery, and to investigate the risk factors and clinical reasons for unplanned readmissions.MethodA multicenter, population‐based study including all patients discharged after index colorectal cancer resection from 2010 to 2020 in Aotearoa New Zealand (AoNZ) was completed. The Ministry of Health National Minimum Dataset was used. Rates of readmission at 30 days and 90 days were calculated. Mixed‐effect logistic regression models were built to investigate factors associated with unplanned readmission. Reasons for readmission were described.ResultsData were obtained on 16,885 patients. Unplanned 30‐day and 90‐day hospital readmission rates were 15.1% and 23.7% respectively. The main readmission risk factors were comorbidities, advanced disease, and postoperative complications. Hospital level variation was not present. Despite risk adjustment, R2 value of models was low (30 days: 4.3%, 90 days: 5.2%). The most common reasons for readmission were gastrointestinal causes (32.1%) and wound complications (14.4%). Rates of readmission did not improve over the 11 years study period (p = 0.876).ConclusionReadmissions following colorectal resections in AoNZ are higher than other comparable healthcare systems and rates have remained constant over time. While patient comorbidities and postoperative complications are associated with readmission, the explanatory value of these variables is poor. To reduce unplanned readmissions, efforts should be focused on prevention and early detection of post‐discharge complications.
Influences of solid content of slurry on the microstructure of CaO crucible prepared by slip casting
Authors: Deng Zirong , Wei Yaowu , Zhou Hui , Tuo Kangan , Wang Yanping , Zhang Yi 
Published: January 2023

AbstractCaO crucible was prepared from Ca(OH)2 slurry via a slip‐casting method in order to avoid hydration problem caused by free CaO during preparation process. The effect of solid content of Ca(OH)2 slurry on microstructure of CaO crucible was investigated. It was found that the rheological property of the slurry was significantly improved when the solid content of the slurry within a certain range. The change of solid content of slurry affects the microstructure of the final products. When the solid content of the slurry was 71 wt%, the manufactured CaO crucible was of smallest mean pore size (2.58 μm) after 1600°C heating treatment. The heated CaO sample was of lower apparent porosity (4.1%) and higher bulk density (2.93 g/cm3). And the size distributions of most CaO grains are between 10 and 40 μm.
Cost‐effective suspension formulation for flexible TiB2 tapes
Authors: Shirey Kaitlyn , Tallon Carolina 
Published: January 2023

AbstractThe manufacturing of ultra‐high temperature ceramic materials has significantly advanced over recent years, allowing for the development of new microstructures, architectures, shapes, and geometries to explore new properties and applications for these materials beyond aerospace. For example, titanium diboride (TiB2) exhibits high electrical and thermal conductivity that could satisfy the needs of battery applications by tailoring its geometry, microstructure, and architecture. In this work, aqueous tape casting of TiB2 has been investigated. Zeta potential measurements and suspension rheology were used to understand the role of dispersant, binder, and plasticizer in the suspension and their interaction with the surface chemistry of the TiB2 particles to develop a stable, homogenous suspension, with minimum additive amounts (0%–2 wt%). Homogeneous, flexible, and strong TiB2 tapes were prepared using suspensions with 30 vol% solids and characterized to compare different compositions, mixing methods, and thicknesses. The characterization shows the tailoring of the properties as a function of the controlled suspension formulation with a minimum amount of additives. Green tapes with 2 wt% dispersants, 1 wt% binder, and 2 wt% plasticizers had similar microstructure to those with half the plasticizer but quintuple Young's modulus (1.96 GPa). The effect on other relevant properties is also discussed.
New semi‐dwarfing alleles with increased coleoptile length by gene editing of gibberellin 3‐oxidase 1 using CRISPR‐Cas9 in barley (Hordeum vulgare L.)
Authors: Cheng Jingye , Hill Camilla , Han Yong , He Tianhua , Ye Xingguo , Shabala Sergey , Guo Ganggang , Zhou Meixue , Wang Ke , Li Chengdao 
Published: January 2023

SummaryThe green revolution was based on genetic modification of the gibberellin (GA) hormone system with “dwarfing” gene mutations that reduces GA signals, conferring shorter stature, thus enabling plant adaptation to modern farming conditions. Strong GA‐related mutants with shorter stature often have reduced coleoptile length, discounting yield gain due to their unsatisfactory seedling emergence under drought conditions. Here we present gibberellin (GA) 3‐oxidase1 (GA3ox1) as an alternative semi‐dwarfing gene in barley that combines an optimal reduction in plant height without restricting coleoptile and seedling growth. Using large‐scale field trials with an extensive collection of barley accessions, we showed that a natural GA3ox1 haplotype moderately reduced plant height by 5–10 cm. We used CRISPR/Cas9 technology, generated several novel GA3ox1 mutants and validated the function of GA3ox1. We showed that altered GA3ox1 activities changed the level of active GA isoforms and consequently increased coleoptile length by an average of 8.2 mm, which could provide essential adaptation to maintain yield under climate change. We revealed that CRISPR/Cas9‐induced GA3ox1 mutations increased seed dormancy to an ideal level that could benefit the malting industry. We conclude that selecting HvGA3ox1 alleles offers a new opportunity for developing barley varieties with optimal stature, longer coleoptile and additional agronomic traits.