bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the microbiota-sparing properties of the anti-staphylococcal antibiotic afabicin
Authors: Nowakowska J , Cameron D R , De Martino A , Kühn J , Le Fresne-Languille S , Leuillet S , Amouzou Y , Wittke F , Carton T , Le Vacon F , Chaves R L , Nicolas-Metral V , Vuagniaux G 
Published: June 2023

Abstract

Background
Antibiotic use is associated with collateral damage to the healthy microbiota. Afabicin is a first-in-class prodrug inhibitor of the FabI enzyme that, when converted to the pharmacologically active agent afabicin desphosphono, demonstrates a staphylococcal-specific spectrum of activity. An expected benefit of highly targeted antibiotics such as afabicin is microbiome preservation.

Objectives
To compare the effects of oral treatment with afabicin and standard-of-care antibiotics upon the murine gut microbiota, and to assess the effects of oral afabicin treatment on the human gut microbiota.

Methods
Gut microbiota effects of a 10 day oral course of afabicin treatment were monitored in mice and compared with clindamycin, linezolid and moxifloxacin at human-equivalent dose levels using 16S rDNA sequencing. Further, the gut microbiota of healthy volunteers was longitudinally assessed across 20 days of oral treatment with afabicin 240 mg twice daily.

Results
Afabicin treatment did not significantly alter gut microbiota diversity (Shannon H index) or richness (rarefied Chao1) in mice. Only limited changes to taxonomic abundances were observed in afabicin-treated animals. In contrast, clindamycin, linezolid and moxifloxacin each caused extensive dysbiosis in the murine model. In humans, afabicin treatment was not associated with alterations in Shannon H or rarefied Chao1 indices, nor relative taxonomic abundances, supporting the findings from the animal model.

Conclusions
Oral treatment with afabicin is associated with preservation of the gut microbiota in mice and healthy subjects.
Knowledge, attitude and practice of healthcare workers towards antibiotic resistance during the COVID-19 pandemic
Authors: Al Sulayyim Hadi , Ismail Rohani , Hamid Abdullah Al , Ghafar Noraini Abdul 
Published: June 2023

Abstract

Background
During the novel Coronavirus 2019 (COVID-19) outbreak, there was an overuse of antibiotics in hospitals. The improper use of antibiotics during COVID-19 has increased antibiotic resistance (AR), which has been reported in multiple studies.

Objectives
To assess the healthcare workers’ (HCWs) knowledge, attitude and practice (KAP) in relation to AR during the era of COVID-19, and identify the associated factors with good knowledge, positive attitude and good practice.

Methods
A cross-sectional design was used to assess the KAP of HCWs in Najran, Kingdom of Saudi Arabia (KSA). A validated questionnaire was used to collect participants’ data, which consisted of the following information; socio-demographics, knowledge, attitude and items for practice. Data were presented as percentages and median (IQR). Mann–Whitney and Kruskal Wallis tests were used to compare them. Logistic regression was used to identify the associated factors linked to KAP.

Results
The study included 406 HCWs. Their median (IQR) knowledge score was 72.73% (27.27%–81.82%), attitude score was 71.43% (28.57%–71.43%) and practice score was 50% (0%–66.67%). About 58.1% of the HCWs stated that antibiotics could be used to treat COVID-19 infection; 19.2% of the participants strongly agreed and 20.7% agreed that during the COVID-19 pandemic, antibiotics were overused at their healthcare institutions. Only 18.5% strongly agreed and 15.5% agreed when asked whether antibiotics used properly for the right indication and duration can still result in AR. The significantly associated factors with good knowledge were nationality, cadre and qualification. A positive attitude was significantly associated with age, nationality and qualification. Good practice was significantly associated with age, cadre, qualification and working place.

Conclusion
Although the HCWs had a positive attitude regarding AR during COVID-19, their knowledge and practice need significant improvement. Implementation of effective educational and training programmes are urgently needed. In addition, further prospective and clinical trial studies are needed to better inform these programmes.
Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development
Authors: Mitrović Ivana , Vučurović Damjan , Al-Ani Laith Khalil Tawfeeq , Mitrović Bojan , Bajić Bojana , Dodić Siniša , Živanov Sonja Tančić 
Published: June 2023

Abstract

Aims
In order to be competitive on the market, the production of biopreparations needs to be optimized, modelled, and assessed in the early stages of its development. The aim of this paper was to optimize medium for the production of Trichoderma harzianum K179 biocontrol agent, to analyze its kinetics at enlarged laboratory scale and finally economic analysis of the production of this high-value product through simulation modelling.

Methods and results
The results showed that the bioprocess of T. harzianum K179 bioagent production in a laboratory bioreactor on the medium with optimal composition (dextrose 10 g l−1, soy flour 6.87 g l−1, K2HPO4 1.51 g l−1, KCl 0.5 g l−1, and MgSO4 × 7H2O 0.5 g l−1), at stirring speed of 1.75 × g and aeration intensity of 1.5 vvm, can be shortened from 96 to 36 h. The results of bioprocess economic analysis showed that with a 25-year project lifetime and an investment payback time of 7.58 years, this project represents an economically viable system.

Conclusions
Complete analysis of the bioprocess of T. harzianum K179 biocontrol agent production showed that the biologically produced preparation can be competitive on the market with synthetic preparations.
Biofilm formation under food-relevant conditions and sanitizers’ tolerance of a Pseudomonas fluorescens group strain
Authors: Maifreni Michela , Di Bonaventura Giovanni , Marino Marilena , Guarnieri Simone , Frigo Francesca , Pompilio Arianna 
Published: June 2023

Abstract

Aims
The aim of this study was to determine the biofilm-forming ability of a strain belonging to the Pseudomonas fluorescens group isolated from the dairy environment under food-relevant conditions. Moreover, the effects of commercial sanitizers against preformed biofilms were assessed both in terms of viability and structure.

Methods and results
The biofilms were formed on polystyrene, stainless steel (SS), and polytetrafluoroethylene (PTFE) in a wide range of temperatures (4–25°C) and were subjected to the action of 10 different sanitizers. The strain under study showed to be a strong biofilm-former regardless of temperature, particularly on polystyrene. The biofilms were mostly sensitive to chlorine and peracetic acid-based sanitizers. For some sanitizers (e.g. amphoteric), a relationship was observed between the material and the tolerance, while the temperature was not statistically significant. The formation of long-term biofilms on SS was also structurally affected by the temperature, showing microcolonies more irregular in shape and with lower cellularity at 4°C compared to 15°C, where the biofilm was more compact and with a high presence of EPS.

Conclusions
The strain belonging to the P. fluorescens group was shown to quickly adhere and form mature biofilm at temperatures and on materials relevant to the food sector; however, biofilms formed under different conditions were differently tolerant to disinfectants.

Significance and impact of the study
Findings from this study could provide a basis for developing targeted sanitation protocols in food plants.
Use of the shuttle box system to determine the effects of hypoxia and food deprivation on the behavioral responses of the rock crab Cancer irroratus Say, 1817 (Decapoda: Brachyura: Cancridae)
Authors: Jiang Qiwu , McGaw Iain J 
Published: June 2023

Abstract
Food-deprivation state (fed, fasted, starved) affected rock crabs physiological and biochemical responses to hypoxia in Cancer irroratus. Fasted and starved crabs were better adapted to deal with hypoxia than fed animals; however, avoidance behavior is usually considered as the first defense to environmental stressors for decapod crustaceans. We examined the effects of food deprivation on the crab’s behavior to hypoxia using the Loligo® shuttle box system, an automated system with a pair of connected water chambers with regulated flow and oxygen level. Crabs (starved, fasted and fed) that were offered a choice of two different oxygen saturations did not appear to actively avoid the hypoxia regimes tested (50% and 20% oxygen saturation). We used novel algorithms to analyze the data and found that crabs altered rheotaxis (movement towards or away from a current of water) and corresponding moving speed as a function of oxygen saturation. The food-deprivation state did influence thigmotaxis (contact with walls/objects when exploring an open space): starved crabs became bolder and more likely to explore open areas of the apparatus. Technological advancements such as the fully automated shuttle box have improved our ability to collect and analyze behavioral data; however, our study also highlighted some of the potential problems of relying solely on such apparatus to study the behavior of benthic crustaceans.
Picturing National History: Turkey’s Popular Nationalism on the Rise Through the 1950s New Print Culture
Authors: Ozguner Emin Artun 
Published: June 2023

Abstract
This article interrogates the role of print culture and visual communication design in the permeation of Turkish national consciousness into everyday practices. It seeks to understand the phenomenon of “banal nationalism,” argued by Michael Billig, in a broader context of cultural production advocated by Tim Edensor. It does this by looking into Turkey’s first liberalization period in the 1950s where a boosting print industry, a standard print language, and high literacy contributed to the daily reproduction of a collective historical past through representations. This period is analyzed through publications like Sunday comic strips, advertorial giveaways, and illustrated history journals that emulate popular American formats in the commodification of history. These are treated as material tools to present and disseminate an imaginary reconciliation of secular modernism with imperial history in a new print culture. This analysis reveals how representations in the foreground of everyday cultural artifacts are used to produce and reproduce difference that designates a distinct national consciousness detached from the realm of state. It also sheds light on the prevalence of identity negotiation and the commoditization of culture in the professionalization of visual communication disciplines in non-Western design paradigms.
Large-scale production of house fly, Musca domestica (Diptera: Muscidae), larvae fed 3 manure types
Authors: Miranda Chelsea D , Cammack Jonathan A , Tomberlin Jeffery K 
Published: June 2023

Abstract
House flies, Musca domestica, L., (Diptera: Muscidae) are well-known pests at animal facilities; however, they can be used for manure biodegradation. Utilizing house flies to process animal manure offers a means to recycle nutrients and reduce contaminants (e.g., pathogens and heavy metals), while also producing multiple revenue streams (e.g., protein for feed, fat for biodiesel, frass as a soil amendment). This study determined house fly larval performance on a larger scale (kilogram of wastes; thousands of larvae; single feeding) as a follow-up to a previous experiment performed at a bench-top scale (g of wastes; hundreds of larvae; incremental feeding). Four thousand larvae were fed 1 kg of swine, dairy, or poultry manure, or a control (Gainesville diet: 50% wheat bran, 30% alfalfa meal, and 20% corn meal). Peak larval weight occurred 4 days after inoculation and no significant difference in development time to first pupariation occurred across diets. However, percent survivorship to pupariation varied, with the highest occurring in Gainesville (74%), swine (73%), and poultry (67%) manure, whereas 50% survived when fed dairy manure. The highest pupal weight was found for those fed Gainesville (27 mg), and similar weights were found for those fed swine (21 mg), dairy (24 mg), and poultry (25 mg) manure. Although using house flies to manage manure has received little consideration in Western countries, other regions have this practice in place. Results may provide insight on differences between small- and large-scale studies, which is valuable for industrialization of this species for waste management and creating a more circular economy.
Coverage of Human Rights Issues in Malawian Newsrooms: Challenges and Prospects
Authors: Mlenga Joe 
Published: June 2023

Abstract
This paper is a study of 14 media houses in Malawi and it looks at coverage of human rights issue from various dimensions. A questionnaire was administered to journalists of diverse levels at these media houses to gather data concerning the research. The targeted media houses are located in the main urban centres of Malawi and include radio, television and newspaper publishers. The findings indicate that training, lack of specialised units in newsrooms, inadequate reference materials and reluctance by officials to give out required information are some of the issues that are hampering coverage of human rights stories in the country. The paper also looks at suggestions made by journalists to help improve reportage of human rights in Malawi. It then makes recommendations based on evidence gathered through the questionnaires on how the media and other concerned stakeholders can work together for better human rights reporting in the country.
Influence of artificial diets on biological characteristics and digestive enzymes of Coccinella septempunctata L.
Authors: Cheng Ying , Yu Yi , Li Wenhong , Li Fengliang 
Published: June 2023

Abstract
In order to improve the rearing of Coccinella septempunctata L. (Coleoptera: Coccinellidae), nutrients such as shrimp, pollen, honey, and lard were added to the basic artificial diet, and the effects of the artificial diet on biological parameters and digestive enzymes were evaluated. The results show that beetles feeding on the supplemented diet exhibited pupation, emergence, fecundity, and hatching rates that were 102.69%, 125.02%, 162.33%, and 119.90% of those supplied with the basic diet, respectively. The addition of shrimp and pollen to the basal diet improved protease, trypsin, chymotrypsin, and aminopeptidase activity in larvae and female adults. The addition of lard improved lipase activity in female adults, and the addition of honey improved invertase activity in adults of both sexes. This study provides guidance for improving the nutritional benefits of ladybug artificial diets.
Variation in North American bumble bee nest success and colony sizes under captive rearing conditions
Authors: Strange James P , Tripodi Amber D , Lindsay Thuy-Tien T , Herndon James D , Knoblett Joyce , Christman Morgan E , Barkan N Pinar , Koch Jonathan B U 
Published: June 2023

Abstract
Of the 265 known bumble bee (Bombus) species, knowledge of colony lifecycle is derived from relatively few species. As interest in Bombus commercialization and conservation grows, it is becoming increasingly important to understand colony growth dynamics across a variety of species since variation exists in nest success, colony growth, and reproductive output. In this study, we reported successful nest initiation and establishment rates of colonies and generated a timeline of colony development for 15 western North American Bombus species, which were captively reared from wild-caught gynes from 2009 to 2019. Additionally, we assessed variation in colony size among 5 western North American Bombus species from 2015 to 2018. Nest initiation and establishment rates varied greatly among species, ranging from 5–76.1% and 0–54.6%, respectively. Bombus griseocollis had the highest rates of nest success across the 11-yr period, followed by B. occidentalis, B. vosnesenskii, and B. huntii. Furthermore, days to nest initiation and days to nest establishment varied among species, ranging from 8.4 to 27.7 days and 32.7 to 47 days. Colony size also differed significantly among species with B. huntii and B. vosnesenskii producing more worker/drone cells than B. griseocollis, B. occidentalis, and B. vancouverensis. Additionally, gyne production differed significantly among species with B. huntii colonies producing more gynes than B. vosnesenskii. Results from this study increase knowledge of systematic nesting biology for numerous western North American Bombus species under captive rearing conditions, which can further improve rearing techniques available to conservationists and researchers.
Sublethal effects of emamectin benzoate on development, reproduction, and vitellogenin and vitellogenin receptor gene expression inThrips hawaiiensis(Thysanoptera: Thripidae)
Authors: Chen Yi-Xin , Tian Hou-Jun , Lin Shuo , Yu Yun , Xie Lian-Cheng , Li Heng , Chen Yong , Lin Tao , Gu Xiao-Jun , Wei Hui 
Published: June 2023

AbstractThrips hawaiiensis (Morgan) (Thysanoptera: Thripidae) is a sap-sucking pest that seriously damages several crops and reduces their economic value. Exposure to low concentrations of insecticides may have a sublethal effect on surviving insects. In order to provide a reference for the rational application of emamectin benzoate, its sublethal effects on the development and reproduction of T. hawaiiensis were evaluated. Pupal development time was significantly shorter in T. hawaiiensis treated with sublethal concentrations of emamectin benzoate (LC10 and LC20) than in control. Female adult longevity and female total longevity were significantly longer following LC20 treatment than in the control and LC10 treatment groups. Nevertheless, male adult longevity and male total longevity were significantly shorter in the LC10 treatment group than in the control and LC20 treatment groups. The sublethal concentration of emamectin benzoate (LC20) significantly shortened the preadult stages and the mean generation. Meanwhile, it significantly increased the finite rate of increase, intrinsic rate of increase, and net reproductive rate. The fecundity was significantly higher after LC20 treatment than after LC10 and control treatments. Compared with the control group, the LC10 and LC20 groups of T. hawaiiensis adults showed a significantly higher expression of the vitellogenin (Vg) and vitellogenin receptor (VgR) genes, which played a key role in increasing their fecundity. These findings suggest that short-term exposure to sublethal concentrations of emamectin benzoate may lead to a resurgence and secondary outbreak of T. hawaiiensis infestation. The results have practical applications for the management of this important and noxious pest.
A New Test on Asset Return Predictability with Structural Breaks
Authors: Cai Zongwu , Chang Seong Yeon 
Published: June 2023

Abstract
This article considers predictive regressions in which a structural break is allowed on an unknown date. We establish novel testing procedures for asset return predictability using empirical likelihood (EL) methods based on weighted score equations. The theoretical results are useful in practice because our unified framework does not require distinguishing whether the predictor variables are stationary or non-stationary. Monte Carlo simulation studies show that the EL-based tests perform well in terms of size and power in finite samples. Finally, as an empirical analysis, we test the predictability of the monthly S&P 500 value-weighted log excess return using various predictor variables.
Wagering with an incomplete deck—refining conservation plans for the New Mexico Meadow Jumping Mouse (Zapus luteus luteus)
Authors: Malaney Jason L , Wilford Caroline R , Woods Jonathan T , Christman Bruce L , Jennings Randy D , Chambers Carol L , Zahratka Jennifer L , Liphardt Schuyler W , Demboski John R , Cook Joseph A 
Published: June 2023

AbstractLimited sampling for imperiled taxa inhibits effective management by obscuring windows into ecological and evolutionary processes and ultimately thwarting thoughtful conservation efforts. We report eight new locations for the endangered New Mexico Meadow Jumping Mouse (Zapus luteus luteus) detected across three states that expand their known distribution. When combined with existing curated museum records, we develop an ensemble species distribution model to evaluate persistence of populations over the next 50 years. Predicted distributions indicate complex future changes, including regional expansion and the likelihood that half of the designated critical habitat areas will be unsuitable by 2070. Three of the newly discovered populations occur where predicted climate conditions suggest extirpation is likely. Importantly, indices of historical sampling efforts show that recognized and potential distributions are mismatched, highlighting vast areas that have been insufficiently surveyed. Ongoing habitat degradation and climate disruption are projected to synergistically erode genetic diversity across four of the five divergent phylogroups. Considering these combined results, a holistic sampling strategy is needed to more completely document the distribution of jumping mice and facilitate genomic analyses aimed at establishing a roadmap for improving our understanding of geographic variation and adaptive potential. Current management efforts are not only costly, but they are lacking key biological insights, essentially wagering actions with an incomplete deck of cards.
Toward a Hybrid Theory of How to Allocate Health-related Resources
Authors: Herlitz Anders 
Published: June 2023

Abstract
How should scarce health-related resources be allocated? This paper argues that values that apply to these decisions fail to always fully determine what we should do. Health maximization and allocation-according-to-need are suggested as two values that should be part of a general theory of how to allocate health-related resources. The “small improvement argument” is used to argue that it is implausible that one alternative is always better, worse, or equal to another alternative with respect to these values. Approaches that rely on these values are thus incomplete. To deal with this, it is suggested that we ought to use incomplete theories in a two-step process. Such a process first discards ineligible alternatives, and, second, uses reasons grounded in collective commitments to identify a unique, best alternative in the remaining set.
Involuntary Childlessness, Suffering, and Equality of Resources: An Argument for Expanding State-funded Fertility Treatment Provision
Authors: Cavaliere Giulia 
Published: June 2023

AbstractAssessing what counts as infertility has practical implications: access to (state-funded) fertility treatment is usually premised on meeting the criteria that constitute the chosen definition of infertility. In this paper, I argue that we should adopt the expression “involuntary childlessness” to discuss the normative dimensions of people’s inability to conceive. Once this conceptualization is adopted, it becomes clear that there exists a mismatch between those who experience involuntary childlessness and those that are currently able to access fertility treatment. My concern in this article is explaining why such a mismatch deserves attention and what reasons can be advanced to justify addressing it. My case rests on a three-part argument: that there are good reasons to address the suffering associated with involuntary childlessness; that people would decide to insure against it; and that involuntary childlessness is characterized by a prima facie exceptional kind of desire.