bluuebunny/crossref_metadata_2025_split huggingface dataset:
Putative adaptive loci show parallel clinal variation in a California‐endemic wildflower
Authors: Hernández Adriana I. , Specht Chelsea D. 
Published: May 2023

AbstractAs the global climate crisis continues, predictions concerning how wild populations will respond to changing climate conditions are informed by an understanding of how populations have responded and/or adapted to climate variables in the past. Changes in the local biotic and abiotic environment can drive differences in phenology, physiology, morphology and demography between populations leading to local adaptation, yet the molecular basis of adaptive evolution in wild non‐model organisms is poorly understood. We leverage comparisons between two lineages of Calochortus venustus occurring along parallel transects that allow us to identify loci under selection and measure clinal variation in allele frequencies as evidence of population‐specific responses to selection along climatic gradients. We identify targets of selection by distinguishing loci that are outliers to population structure and by using genotype–environment associations across transects to detect loci under selection from each of nine climatic variables. Despite gene flow between individuals of different floral phenotypes and between populations, we find evidence of ecological specialization at the molecular level, including genes associated with key plant functions linked to plant adaptation to California's Mediterranean climate. Single‐nucleotide polymorphisms (SNPs) present in both transects show similar trends in allelic similarity across latitudes indicating parallel adaptation to northern climates. Comparisons between eastern and western populations across latitudes indicate divergent genetic evolution between transects, suggesting local adaptation to either coastal or inland habitats. Our study is among the first to show repeated allelic variation across climatic clines in a non‐model organism.
The effect of sleep positional therapy on nocturnal gastroesophageal reflux measured by esophageal pH‐impedance monitoring
Authors: Schuitenmaker Jeroen M. , Kuipers Thijs , Schijven Marlies P. , Smout André J. P. M. , Fockens Paul , Bredenoord Albert J. 
Published: May 2023

AbstractBackground & AimsThe aim of the study was to evaluate the effect of an electronic positional therapy wearable device on nocturnal gastroesophageal reflux measured by pH‐impedance reflux monitoring.MethodsWe performed a single‐center, prospective, interventional study in 30 patients with nocturnal reflux symptoms and a nocturnal esophageal acid exposure time (AET) ≥1.5% measured off acid‐suppressive medication by ambulatory pH‐impedance reflux monitoring. Patients were treated with an electronic positional therapy wearable device for 2 weeks. The device vibrates in the right lateral decubitus position so it conditions patients to avoid that sleep position. After 2 weeks treatment, the pH‐impedance study was repeated. Primary outcome was the change in nocturnal AET. Secondary outcomes include change in number of reflux episodes and reflux symptoms.ResultsComplete data were available for 27 patients (13 females, mean age 49.8 years). The median nocturnal AET decreased from 6.0% (IQR, 2.3–15.3) to 3.1% (0.1–10.8) after 2 weeks of treatment (p = 0.079). The number of reflux episodes was significantly reduced after 2 weeks of treatment (baseline: 8.0 (3.0–12.3) vs. end: 3.0 (1.0–8.0); p = 0.041). Treatment led to a statistically significant decrease in time spent in right lateral decubitus position (baseline: mean 36.9% ± 15.2% vs. end: 2.7% ± 8.2%; p = <0.001) and an increase in the left lateral decubitus position (baseline 29.2% ± 14.8% vs. end: 63.3% ± 21.9%; p = <0.001). Symptom improvement was reported by 70.4% of the patients.ConclusionsSleep positional therapy using an electronic wearable device promotes sleeping in the left lateral decubitus position and improves reflux parameters measured by pH‐impedance reflux monitoring.
SlMYB99‐mediated auxin and abscisic acid antagonistically regulate ascorbic acids biosynthesis in tomato
Authors: Xu Xin , Huang Baowen , Fang Xu , Zhang Qiongdan , Qi Tiancheng , Gong Min , Zheng Xianzhe , Wu Mengbo , Jian Yongfei , Deng Jie , Cheng Yulin , Li Zhengguo , Deng Wei 
Published: May 2023

Summary

Ascorbic acid (AsA) is a water‐soluble antioxidant that plays important roles in plant development and human health. Understanding the regulatory mechanism underlying AsA biosynthesis is imperative to the development of high AsA plants.

In this study, we reveal that the auxin response factor SlARF4 transcriptionally inhibits SlMYB99, which subsequently modulates AsA accumulation via transcriptional activation of AsA biosynthesis genes GPP, GLDH, and DHAR. The auxin‐dependent transcriptional cascade of SlARF4‐SlMYB99‐GPP/GLDH/DHAR modulates AsA synthesis, while mitogen‐activated protein kinase SlMAPK8 not only phosphorylates SlMYB99, but also activates its transcriptional activity. Both SlMYB99 and SlMYB11 proteins physically interact with each other, thereby synergistically regulating AsA biosynthesis by upregulating the expression of GPP, GLDH, and DHAR genes.

Collectively, these results demonstrate that auxin and abscisic acid antagonistically regulate AsA biosynthesis during development and drought tolerance in tomato via the SlMAPK8‐SlARF4‐SlMYB99/11 module.

These findings provide new insights into the mechanism underlying phytohormone regulation of AsA biosynthesis and provide a theoretical basis for the future development of high AsA plants via molecular breeding.
The specific value of upgrading to left bundle branch area pacing in patients with pacing‐induced cardiomyopathy or non‐pacing‐induced cardiomyopathy related upgrade status: A retrospective study
Authors: Shan Yu , Lin Maoning , Sun Yaxun , Zhang Jiefang , Jiang Hangpan , Fu Guosheng , Zhang Wenbin , Wang Min 
Published: May 2023

AbstractAimsLeft bundle branch area pacing (LBBaP) upgrade can improve cardiac function and clinical outcomes in patients with pacing‐induced cardiomyopathy (PICM), but the specific value of LBBaP upgrade, especially compared with the cardiac function level before right ventricular pacing (RVP) in patients with PICM and non‐pacing‐induced cardiomyopathy‐related upgrade status (Non‐PICMUS) is still unknown.MethodsThis study retrospectively enrolled 70 patients with LBBaP upgrade (38 patients with PICM and 32 patients with Non‐PICMUS). All upgrade patients experienced three stages: before RVP (Pre‐RVP), before LBBaP upgrade (Pre‐LBBaP), and after LBBaP upgrade (Post‐LBBaP). QRS duration (QRSd), lead parameters, echocardiographic indicators, and clinical outcomes evaluation were recorded at multiple time points.ResultsAt the follow‐up of 12 months, for PICM patients, left ventricular ejection fraction (LVEF) significantly increased from 36.6% ± 7.2% to 51.3% ± 8.7% Post‐LBBaP (p < .001), and left ventricular end‐diastolic diameter (LVEDD) significantly decreased from 61.5 ± 6.4 mm to 55.2 ± 6.5 mm Post‐LBBaP (p < .001), but they both failed to restore the level Pre‐RVP (both p < .001). For PICM patients, New York Heart Association (NYHA) classification, the number of moderate‐to‐severe heart failure (NYHA III–IV), and diuretics using rate after the LBBaP upgrade also could not restore to the level Pre‐RVP (all p < .001). At the follow‐up of 12 months, Non‐PICMUS patients after the LBBaP upgrade had no significant improvement in LVEF, LVEDD, and NYHA classification (all p > .05).ConclusionLBBaP upgrade effectively improved the cardiac function and clinical outcomes in PICM patients, but its effectiveness seemed to be limited as the deteriorated cardiac function cannot be completely reversed. For Non‐PICMUS patients, the cardiac function and clinical outcomes Post‐LBBaP had no significant improvement.
Border Disputes: Recent Debates along the Perception–Cognition Border
Authors: Clarke Sam , Beck Jacob 
Published: May 2023

AbstractThe distinction between perception and cognition frames countless debates in philosophy and cognitive science. But what, if anything, does this distinction actually amount to? In this introductory article, we summarize recent work on this question. We first briefly consider the possibility that a perception‐cognition border should beeliminatedfrom our scientific ontology, and then introduce and critically examine five positive approaches to marking a perception–cognition border, framed in terms ofphenomenology, revisability, modularity, format,andstimulus‐dependence.
Creativity in policy capacity: Organizational and individual determinants
Authors: Fleischer Julia , Wanckel Camilla 
Published: May 2023

AbstractCreativity is a crucial part of policy capacity in governments. Existing studies on creative behavior in the public sector assess employees' openness to new ideas and creative solutions, and they confirm the relevance of organizational and individual determinants for pro‐creativity attitudes. Yet we lack systemic evidence on the explicit level of work‐related creativity among policy officials in government organizations. At the same time, novel technologies and particularly social networking services change the working environment of policy officials radically, alter organizational features, and may also yield crucial individual effects. Our study analyses “policy creativity” of policy officials in three European governments. We demonstrate the importance of organizational and individual features, including the stress triggered by using social networking services. Our study captures officials' creativity explicitly and adds to debates on creativity and innovation in the public sector as well as the micro‐level foundations of the digital transformation in the public sector.
Antecubital perforating vein stent‐grafting for radiocephalic arteriovenous fistula recovery: A case report
Authors: Mendes Daniel , Almeida Paulo , Pinelo Andreia , Castro João , Norton‐de‐Matos António 
Published: May 2023

AbstractStent‐grafts have been increasingly used in hemodialysis arteriovenous accesses, particularly in recurrent stenosis or in cases of vein rupture after percutaneous transluminal angioplasty (PTA). Although they limit neointimal hyperplasia, stenosis development at stent edges remains a concern. Despite their advantages, they are seldom used on the forearm veins due to fracture risk associated with elbow motion and the potential to limit cannulation sites. This report presents a novel application of stent‐grafts in salvaging a radio‐cephalic arteriovenous fistula in an 84‐year‐old male to treat a single outflow path at the elbow through a stenosed antecubital perforating vein, after failed PTA. The vascular access remained patent 18 months after the procedure, with no need for additional treatments at this target lesion, even though a PTA was required for juxta‐anastomotic stenosis. This report highlights a possible further use of covered stents in arteriovenous vascular accesses.
The role of liquidity in monetary policy transmission: Evidence from Indian banks1
Authors: Ansari Md Gyasuddin , Sensarma Rudra 
Published: May 2023

AbstractThe role of bank liquidity in monetary policy transmission has received insufficient attention in the literature. Faced with monetary tightening, banks with more liquidity can sell off securities and protect their loan portfolios. We test this proposition using panel data for Indian banks during 2005–2020. Employing dynamic threshold panel regressions with liquid assets as the threshold variable, we show that bank lending declines with monetary policy tightening in low liquidity regimes, but not in high liquidity regimes. We also find evidence for different portfolio reallocation behaviour by banks in high versus low liquidity regimes in response to monetary policy changes.
The effect of late‐onset CMV infection on the outcome of renal allograft considering initial graft function
Authors: Shahmirzadi Mohammadreza R. , Gunaratnam Lakshman , Jevnikar Anthony M. , Luke Patrick , House Andrew A. , Silverman Michael S. , Hosseini‐Moghaddam Seyed M. 
Published: May 2023

AbstractBackgroundDelayed graft function (DGF) increases the renal allograft failure risk. Late‐onset Cytomegalovirus (CMV) infection's effect on the association between DGF and allograft failure has not been determined.MethodsIn this retrospective cohort, we included all renal allograft recipients at London Health Sciences Centre from January 1, 2014 to December 30, 2017, and continued clinical follow‐up until February 28, 2020. We determined whether late‐onset CMV infection affects the association between DGF and allograft failure in stratified and Cox proportional hazard analyses.ResultsOf 384 patients (median age [interquartile range]: 55 [43.3–63]; 38.7% female), 57 recipients (14.8%) were diagnosed with DGF. Patients with DGF were at a greater risk of CMV infection than patients without DGF (22.8% vs. 11.3%, p = .017). Late‐onset CMV infection (odds ratio [OR]: 4.7, 95% CI: 2.07–10.68) and rejection (OR: 9.59, 95% CI: 4.15–22.16) significantly increased the risk of allograft failure in recipients with DGF. Patients with DGF had a significantly greater risk of graft failure than those without DGF (17.5% vs. 6.1%, p = .007). In the adjusted Cox hazard model, CMV infection significantly increased the risk of allograft failure (aHR: 3.19, 95% CI: 1.49–6.84).ConclusionLate‐onset CMV infection considerably increased the risk of graft failure in patients with DGF. A hybrid preventive model including prophylaxis followed by CMV‐specific cell‐mediated immunity monitoring may decrease the risk of allograft failure in recipients with DGF.
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Effect of vincristine on intraocular pressure and tear fluid oxidative stress biomarkers in canine transmissible venereal tumor
Authors: Keyvanfard Ghazal , Cheraghi Hadi , Aryaei Tabar Hamid 
Published: May 2023

AbstractBackgroundThe ocular side effects of cancer chemotherapeutic drugs are relatively uncommon. Nonetheless, the ocular system has a potentially high sensitivity to toxic substances. This study proposed a framework to assess the effect of vincristine chemotherapy on intraocular pressure, tear protein, and oxidative stress in canines with transmissible venereal tumor (TVT).MethodsThe study group comprised 10 dogs with TVT, whose diagnosis was based on cytology, and all dogs were treated with vincristine for 4 weeks. Each animal was given a complete ophthalmic examination, followed by a standard Schirmer tear test. Before and 20 min after administering vincristine, intraocular pressure (IOP) was measured in the eyes with a noncontact tonometer. At any of the times mentioned, tear samples were collected using the Schirmer test procedure and were subjected to protein analysis—oxidative stress index (OSI), total antioxidant capacity (TAC), total oxidant status (TOS), nitric oxide (NO), and malondialdehyde (MDA) were determined, and standard statistical analysis was applied.ResultsNo significant differences were found in protein in tears, but mean Pre and Postinjection IOP revealed a significant decrease in the eyes each week. Also, results indicated significant differences in oxidative stress markers: increased OSI, NO, and MDA, and reduced TAC.ConclusionThe importance of an increase in oxidative stress levels in the tears of vincristine‐treated patients should be taken seriously, as it appears to play a role in the pathogenesis of eye disease. Therefore, during the treatment weeks prior to prescribing vincristine, eye diseases should be evaluated and considered.
Migration of di(2‐ethylhexyl) phthalate, diisononylcyclohexane‐1,2‐dicarboxylate and di(2‐ethylhexyl) terephthalate from transfusion medical devices in labile blood products: A comparative study
Authors: Thelliez Aurélie , Sumian Chryslain , Chazard Emmanuel , Reichenberg Stefan , Lecoeur Marie , Decaudin Bertrand 
Published: May 2023

AbstractBackground and ObjectivesPolyvinyl chloride (PVC) plasticized with di(2‐ethylhexyl) phthalate (DEHP) is a widely used material for medical transfusion devices. Not covalently bound to PVC, DEHP can migrate into blood products during storage. Recognized as an endocrine disruptor and raising concerns about its potential carcinogenicity and reprotoxicity, DEHP is gradually being withdrawn from the medical device market. Therefore, the use of alternative plasticizers, such as diisononylcyclohexane‐1,2‐dicarboxylate (DINCH) and di(2‐ethylhexyl) terephthalate (DEHT), as potential candidates for the replacement of DEHP in medical transfusion devices has been investigated. The purpose of this study was to evaluate the quantity of PVC‐plasticizers in the blood components according to their preparation, storage conditions and in function of the plasticizer.Materials and MethodsWhole blood was collected, and labile blood products (LBPs) were prepared by the buffy‐coat method with a PVC blood bag plasticized either with DEHP, DINCH or DEHT. DINCH and DEHT equivalent concentrations were quantified in LBPs by liquid chromatography–tandem mass spectrometry or coupled with UV and compared to DEHP equivalent concentrations.ResultsThe plasticizer equivalent concentration to which a patient is exposed during a transfusion depends on the preparation of LBPs as well as their storage conditions, that is, temperature and storage time. At day 1, for all LBPs, the migration of DEHP is 5.0 and 8.5 times greater than DINCH and DEHT, respectively. At the end of the 49 days storage period, the DEHP equivalent concentration in red blood cells concentrate is statistically higher when compared to DINCH and DEHT, with maximal values of 1.85, 1.13 and 0.86 μg/dm2/mL, respectively.ConclusionIn addition to lower toxicity, transfused patients using PVC‐DEHT or PVC‐DINCH blood bags are less exposed to plasticizers than using PVC‐DEHP bags with a ranging exposure reduction from 38.9% to 87.3%, due to lower leachability into blood components.
MiR-128 and MiR-195 Modulate Ligand-Dependent Downregulation of Guanylyl Cyclase/Atrial Natriuretic Peptide Receptor-A
Authors: Ramasamy Chandramohan , Khurana Madan , Mani Indra , Kumar Prerna , Pandey Kailash 
Published: May 2023

Purpose of Study Atrial and brain natriuretic peptides (ANP, BNP) are circulating hormones of cardiac origin that play an important role in regulating blood pressure (BP) and cardiovascular homeostasis. The aim of this study was to investigate the role of natriuretic peptides (NPs) and microRNAs (miRs) in the ANP-dependent downregulation of NPRA, guanylyl cyclase (GC) activity, and cGMP accumulation, utilizing recombinant and/or native endogenous receptors. Methods Human embryonic kidney-293 (HEK-293) cells expressing recombinant receptor and MA-10 cells harboring native endogenous NPRA were cultured and pretreated with 100 nM ANP and BNP to determine 125I-ANP binding to NPRA. Cells were pretreated with 1 μM A71915 (GC inhibitor) to determine the effect on GC activity and with KT-5823 (PKG inhibitor) to examine the involvement of PKG in the regulation of NPRA. To confirm the role of miR-128 and miR-195 in NPRA regulation, the recombinant HEK-293 cells were transfected with the corresponding miRNA precursor sequences cloned into pCMV-MIR-GFP vector and treated with ANP for varying time periods. To estimate the visual production of cGMP, we performed immunofluorescence of cGMP. Results Treatment with ANP (100 nM) for 12 h led to an 80% decrease in 125I-ANP binding of NPRA, and BNP decreased it by 62%. Neither 100 nM c-ANF (truncated ANP) nor C-type natriuretic peptide (CNP) had any effect. ANP (100 nM) treatment also decreased GC activity by 68% and intracellular accumulation cGMP by 45%, while the NPRA antagonist A719151 (1 μM) almost completely blocked ANP-dependent downregulation of NPRA. Treatment with the protein kinase G (PKG) stimulator 8-(4-chlorophenylthio)-cGMP (CPT-cGMP) (1 μM) caused a significant increase in 125I-ANP binding, whereas the PKG inhibitor KT 5823 (1 μM) potentiated the effect of ANP on the downregulation of NPRA. The transfection of miR-128 significantly reduced NPRA protein levels by three-fold compared to control cells. Conclusions The present findings suggest that ligand-dependent mechanisms involving miRs could play an important role in the regulation of GC-coupled receptor, NPRA, which may have important implications in the cardiovascular health and disease. This research work was supported by the National Institutes of Health grants (HL062147 and DK133833). This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
Regulation of human sodium-dependent vitamin C transporter-2 function in neuronal cells by ubiquitin E3 ligase Nedd4-1
Authors: Subramanian Veedamali , Teafatiller Trevor , Kitazawa Masashi , Agrawal Anshu 
Published: May 2023

The ubiquitin pathway regulates the functional expression of several transporters in many cellular systems. However, currently, nothing is known about the role of ubiquitination E3 ligase, neural precursor cell expressed developmentally down-regulated gene 4 (Nedd4-1) in regulating human vitamin C transporter-2 (hSVCT2, the product of the SLC23A2 gene) in neuronal cells. Nedd4-1 has been identified as a putative interactor with hSVCT2 and in the brain, hSVCT2 is predominantly expressed and mediates the cellular uptake of ascorbic acid (AA). Our aim in this investigation was to address this issue using in vitro (SH-SY5Y and HEK-293 cells), and in vivo (mouse) models, together with an array of physiological, cellular and molecular biological approaches. The results showed that the expression of Nedd4-1 in neuronal samples (human and mouse hippocampi and SH-SY5Y cells) is markedly higher than that of Nedd4-2. Interestingly, Nedd4-1 expression was shown to be significantly higher in the hippocampi of patients with Alzheimer’s disease (AD) and a J20 mouse model of AD. In addition, Nedd4-1-GFP and hSVCT2-DsRed co-localized in intracellular vesicles and at the cell membrane in HEK-293 cells. Further, the co-expression of Nedd4-1 with hSVCT2 showed a marked decrease in AA uptake. In contrast, Nedd4-1 siRNA knockdown significantly increased the AA uptake. Further, we have mutated a classical Nedd4 protein interacting motif (‘PPXY’) within the hSVCT2 polypeptide and observed significantly reduced AA uptake due to the intracellular retention of the hSVCT2 mutant compared to hSVCT2 wild-type. Collectively, our data demonstrate that Nedd4-1 dependent ubiquitination regulates hSVCT2 functional expression in neuronal cells. Supported by NIH grant DK 107474 This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.