bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of an anaplastic thyroid cancer stratification signature to guide immune therapy selection and validation of the pivotal gene HLF through in vitro experiments
Authors: Pengping Li , Kexin Yin , Yuwei Xie , Ke Sun , Rongguo Li , Zhenyu Wang , Haigang Jin , Shaowen Wang , Yuqing Huang 
Published: January 2025

IntroductionWhile most thyroid cancer patients have a favorable prognosis, anaplastic thyroid carcinoma (ATC) remains a particularly aggressive form with a median survival time of just five months. Conventional therapies offer limited benefits for this type of thyroid cancer. Our study aims to identify ATC patients who might bene t from immunotherapy.MethodsOur study uses multiple algorithms by R4.2.0, and gene expression and clinical data are collected from TCGA, GEO and local cohort. In vitro experiments, such as western blot and immunofluorescence staining, are performed.ResultsUsing a set of five genes uniquely expressed across various types of thyroid cancer, we developed a machine-learning model to distinguish each type within the GEO dataset of thyroid cancer patients (GSE60542, GSE76039, GSE33630, GSE53157, GSE65144, GSE29265, GSE82208, GSE27155, GSE58545, GSE54958, and GSE32662). These genes allowed us to stratify ATC into three distinct groups, each exhibiting significantly different responses to anti-PD1 therapy as determined by consensus clustering. Through weighted gene co-expression network analysis (WGCNA), we identified 12 differentially expressed genes closely associated with immunotherapy outcomes. This led to the creation of a refined signature for predicting ATC’s immune responsiveness to anti-PD1 therapy, which was further validated using thyroid cancer cohorts from TCGA and nine melanoma cohorts from clinical trials. Among the 12 genes, HLF stood out due to its strong association with various cancer hallmarks.DiscussionOur study revealed that HLF impedes ATC progression by down-regulating the epithelial-to-mesenchymal transition (EMT) pathway, reducing T cell exhaustion, and increasing sensitivity to sorafenib, as demonstrated through our in-vitro experiments.
Loss of Parp7 increases type I interferon signalling and reduces pancreatic tumour growth by enhancing immune cell infiltration
Authors: Kannen Vinicius , Rasmussen Marit , Das Siddhartha , Giuliana Paolo , Izzati Fauzia N. , Choksi Hani , Erlingsson Linnea A. M. , Olafsen Ninni E. , Åhrling Samaneh S. , Cappello Paola , Teino Indrek , Maimets Toivo , Jaudzems Kristaps , Gulbinas Antanas , Dambrauskas Zilvinas , Edgar Landon J. , Grant Denis M. , Matthews Jason 
Published: January 2025

BackgroundPancreatic ductal adenocarcinoma (PDAC) is one of the most lethal forms of cancer, and despite low incidence rates, it remains the sixth leading cause of cancer related deaths worldwide. Immunotherapy, which aims to enhance the immune system’s ability to recognize and eliminate cancer cells, has emerged as a promising approach in the battle against PDAC. PARP7, a mono-ADP-ribosyltransferase, is a negative regulator of the type I interferon (IFN-I) pathway and has been reported to reduce anti-tumour immunity.MethodsWe used murine pancreatic cancer cells, CR705, CRISPR/Cas9, in vivo tumour models and spectral flow cytometry to determine the role of PARP7 in pancreatic tumour growth.ResultsLoss of Parp7 elevated the levels of interferon stimulated gene factor 3 (ISGF3) and its downstream target genes, even in the absence of STING. Cancer cells knocked out for Parp7 (CR705Parp7KO) produced smaller tumours than control cells (CR705Cas9) when injected into immunocompetent mice. Transcriptomic analyses revealed that CR705Parp7KO tumours had increased expression of genes involved in immunoregulatory interactions and interferon signalling pathways. Characterization of tumour infiltrating leukocyte (TIL) populations showed that CR705Parp7KO tumours had higher proportions of natural killer cells, CD8+ T cells and a lower proportion of anti-inflammatory macrophages (M2). The overall TIL profile of CR705Parp7KO tumours was suggestive of a less suppressive microenvironment.ConclusionsOur data show that loss of Parp7 reduces PDAC tumour growth by increasing the infiltration of immune cells and enhancing anti-tumour immunity. These findings provide support to pursue PARP7 as a therapeutic target for cancer treatment.
Case report: A successful case of targeted immunotherapy for locally advanced pancreatic cancer under non-surgical conditions
Authors: Bi Yuanbo , Yv Haotian , Ma Xiaopeng , Chen Shengxiong 
Published: January 2025

IntroductionLocally advanced pancreatic cancer (LAPC) is a borderline unresectable malignancy that presents significant treatment challenges. The management of LAPC remains a complex issue, particularly in patients who are not eligible for surgical resection.CaseHere, we report the case of a 60-year-old woman diagnosed with LAPC through pathological biopsy who subsequently underwent targeted immunotherapy following the failure of a gemcitabine, oxaliplatin, and S-1 (G&amp;S) chemotherapy regimen.InterventionBased on next-generation sequencing (NGS), the patient’s treatment regimen was adjusted to include albumin-bound paclitaxel and capecitabine chemotherapy, along with the PD-1 inhibitor camrelizumab (200 mg/cycle) for six cycles. Throughout the treatment period, the patient consistently declined surgical intervention. Imaging studies, including an upper abdominal computed tomography (CT), revealed the formation of a calcified layer surrounding the cancerous tissue in the pancreatic head. Remarkably, the patient has shown stable disease and no evidence of metastasis since the initiation of targeted immunotherapy.ConclusionThis case highlights the potential of targeted immunotherapy for the treatment of LAPC, particularly in non-surgical patients. A personalized approach guided by NGS, combined with immunotherapy, is an effective alternative to traditional treatment strategies for managing this challenging malignancy.
Optimizing bone-metal implant interfaces: the role of bio-ceramic coatings in improving stability and tissue metabolism
Authors: Balasubramani Geetha , J. Premkumar , J. Paul Pradeep 
Published: January 2025

IntroductionBone replacement is often necessary for patients experiencing pain or swelling in the knee or limb bone region due to osteoarthritis and other bone- related diseases. During surgery, a new bone implant made of metal-on-metal (titanium, cobalt-chromium) or polymer-on-metal (polyethylene on titanium) is used. A significant drawback of these implants is the accumulation of metal or polymer debris, leading to inflammation and infections. Infections or inflammation caused by bacterial adherence to the implant surface result in biofilm formation at the implantation site. Additionally, infections can arise from metal debris generated by the friction and movement of the knee joint, known as implant-associated infections.MethodsThis research aims to develop a bio-ceramic- based composite coating for metal implants. The composite comprises beta- tricalcium phosphate, pectin, gelatine, and polyvinylpyrrolidone (PVP) applied to a 12 mm cortical titanium screw. This coating is designed to enhance the biocompatibility, antibacterial properties, and anti-inflammatory activities of the bone screw, promoting cell growth around the implant and creating a viable environment at the implantation site.Results and DiscussionPrimary characterization of the composite coating materials was conducted using Scanning Electron Microscopy (SEM) with Energy Dispersive X-ray (EDX) analysis and Fourier Infrared Spectroscopy (FTIR). In-vitro antibacterial testing, anti-inflammatory testing, and degradation.
Effect of breathing exercises to prevent pulmonary complications in patients undergoing coronary artery bypass graft surgery: a prospective randomized controlled trials study protocol
Authors: Li Chao , Zhang Ping , Zhang Zichang , Qi Delin , Li Hongli 
Published: January 2025

PurposeTo study the effects of breathing exercises on preventing pulmonary complications in patients undergoing coronary artery bypass graft surgery.MethodsObserving whether preoperative breathing exercises can reduce the incidence of postoperative pulmonary complications in patients undergoing coronary artery bypass graft surgery; observing whether these exercises can improve postoperative arterial oxygen pressure, oxygen saturation, and the distance walked in a six-minute walk test after surgery; as well as reduce hospital stay duration, lower treatment costs, and improve the quality of life as measured by the Short Form-36 Health Survey (SF-36).DesignThe study population includes patients undergoing coronary artery bypass graft surgery under general anesthesia; the research center is Capital Medical University Xuanwu Hospital; the sample size is 120. Preoperative standardized breathing exercises are utilized, and the incidence of postoperative pulmonary complications, postoperative arterial blood gases, oxygen saturation, six-minute walk test distances, and comparisons of hospital stay durations and costs will be observed.
Study of dynamic brain function in irritable bowel syndrome via Hidden Markov Modeling
Authors: Jing Chuan , Liu Tianci , Li Qingzhou , Zhang Chuan , Sun Baijintao , Yang Xuezhao , You Yutao , Liu Jixin , Yang Hanfeng 
Published: January 2025

Background and purposeIrritable bowel syndrome (IBS) is a common bowel-brain interaction disorder whose pathogenesis is unclear. Many studies have investigated abnormal changes in brain function in IBS patients. In this study, we analyzed the dynamic changes in brain function in IBS patients using a Hidden Markov Model (HMM).MethodsResting-state functional magnetic resonance imaging (rs-fMRI) data and the clinical characteristics of 35 patients with IBS and 31 healthy controls (HCs) were collected. The rs-fMRI data of all participants were analyzed using HMM to identify recurrent brain activity states that evolve over time during the resting state. Additionally, the temporal properties of these HMM states and their correlations with clinical scale scores were examined.ResultThis study utilized the Hidden Markov Model (HMM) method to identify six distinct HMM states. Significant differences in fractional occupancy (FO) and lifetime (LT) were observed in states 5 and 6 between the IBS and HCs. The state transition probabilities differed between IBS and HCs, with an increased probability of transitioning from state 2 to state 6 in IBS patients. The reconfiguration of HMM states over time scales in IBS patients was associated with abnormal activity in the default mode network (DMN), sensorimotor network (SMN), and cingulo-opercular network (CON).ConclusionThis study offers novel insights into the dynamic reorganization of brain activity patterns in IBS and elucidates potential links between these patterns and IBS-related emotional regulation and symptom experience, thereby contributing to a deeper understanding of the neural mechanisms underlying IBS.
Partial directed coherence analysis of resting-state EEG signals for alcohol use disorder detection using machine learning
Authors: Mohd Nazri Ainul Khairiyah , Yahya Norashikin , Khan Danish M. , Mohd Radzi Noor'Izni Zafirah , Badruddin Nasreen , Abdul Latiff Abdul Halim , Abdulaal Mohammed J. 
Published: January 2025

IntroductionExcessive alcohol consumption negatively impacts physical and psychiatric health, lifestyle, and societal interactions. Chronic alcohol abuse alters brain structure, leading to alcohol use disorder (AUD), a condition requiring early diagnosis for effective management. Current diagnostic methods, primarily reliant on subjective questionnaires, could benefit from objective measures.MethodThe study proposes a novel EEG-based classification approach, focusing on effective connectivity (EC) derived from resting-state EEG signals in combination with support vector machine (SVM) algorithms. EC estimation is performed using the partial directed coherence (PDC) technique. The analysis is conducted on an EEG dataset comprising 35 individuals with AUD and 35 healthy controls (HCs). The methodology evaluates the efficacy of connectivity features in distinguishing between AUD and HC and subsequently develops and assesses an EEG classification technique using EC matrices and SVM.ResultThe proposed methodology demonstrated promising performance, achieving a peak accuracy of 94.5% and an area under the curve (AUC) of 0.988, specifically using frequency bands 29, 36, 45, 46, and 52. Additionally, feature reduction techniques applied to the PDC adjacency matrices in the gamma band further improved classification outcomes. The SVM-based classification achieved an accuracy of 96.37 ± 0.45%, showcasing enhanced performance through the utilization of reduced PDC adjacency matrices.DiscussionThese results highlight the potential of the developed algorithm as a robust diagnostic tool for AUD detection, enhancing precision beyond subjective methods. Incorporating EC features derived from EEG signals can inform tailored treatment strategies, contributing to improved management of AUD.
The association between dietary creatine intake and cancer in U.S. adults: insights from NHANES 2007–2018
Authors: Jiang Junhui , Zhao Hu , Chen Jiong , Du Junhao , Ni Weixiang , Zheng Baohua , Wu Junhong , Xiao Chunhong 
Published: January 2025

BackgroundCreatine has anti-inflammatory, antioxidant, and immunomodulatory effects. However, its impact on tumors remains uncertain.MethodsThis study used data from the National Health and Nutrition Examination Survey (NHANES) from 2007 to 2018 to investigate the relationship between dietary creatine intake and cancer in American adults. A total of 25,879 participants aged 20 years and older were included, and their medical information, dietary creatine intake, and covariates were collected. Multiple logistic regression models were used to assess the relationships between age, dietary creatine intake, and cancer risk. Restricted cubic spline (RCS) analysis explored the nonlinear relationships between dietary creatine intake, age, and cancer prevalence.ResultsRCS analysis revealed a linear, negative association between dietary creatine intake and cancer risk. For each standard deviation (SD) increase in dietary creatine intake, cancer risk decreased by 5% (adjusted odds ratio (OR) = 0.95, 95% CI: 0.91–0.99, p = 0.025). This negative association was strongest among males (adjusted OR = 0.93, 95% CI: 0.88–0.99, p = 0.021) and overweight participants (adjusted OR = 0.92, 95% CI: 0.84–0.99, p = 0.044). Interaction results indicated specific age group effects. Further analysis showed that higher dietary creatine intake was significantly inversely associated with cancer risk among older adults (adjusted OR = 0.86, 95% CI: 0.77–0.97, p = 0.014). RCS analysis revealed a linear, positive correlation between age and cancer risk. For each SD increase in age, cancer risk increased by 3.27 times (adjusted OR = 3.27, 95% CI: 3.07–3.48, p &lt; 0.001).ConclusionThese findings suggest that higher dietary creatine intake may reduce cancer risk in a nationally representative adult population. Further prospective studies are needed to clarify the relationship between dietary creatine intake and cancer risk.
The efficacy and safety of half-dose glucocorticoids combined with rituximab versus high-dose glucocorticoids for initial treatment of minimal change disease: a single-center experience
Authors: Li Xueting , Yan Peng , Zhang Lu , Qiao Wei , Xue Zhengbiao , Fang Xiangdong , Ke Ben , Zhu Shuying 
Published: January 2025

BackgroundMinimal change disease (MCD) is a podocytopathy more commonly seen in children, but it also accounts for 10%–25% of adult nephrotic syndrome. High-dose oral glucocorticoids were recommended for initial treatment of MCD. However, long-term use of systemic corticosteroids is associated with significant adverse events, such as steroid-induced diabetes and infections. The aim of this study was to investigate the clinical efficacy and safety of half-dose glucocorticoids combined with rituximab (RTX) for the initial treatment of MCD.MethodsWe recruited 74 patients with MCD confirmed by renal biopsy. Twenty patients were treated with RTX alone with 1000 mg at d1 and d15, 28 patients received half-dose prednisolone (0.5 mg/kg) per day combined with RTX with 1000 mg at d1, and 26 patients received high-dose prednisolone (1 mg/kg) per day. Treatment responses, including complete remission (CR) and partial remission (PR), and outcome adverse events such as steroid-induced diabetes and infections were compared among the three groups after 12 months of follow-up.ResultsAt the 12-month follow-up, the CR rates were 50%, 96.4%, and 96.2% for the RTX group, half-dose prednisolone combined with RTX group, and high-dose prednisolone group, respectively. There was no statistical difference between the half-dose prednisolone combined with RTX group and high-dose prednisolone group on CR and PR and kidney function (P &gt; 0.05). Compared with the high-dose prednisolone group, the half-dose prednisolone combined with RTX group had a reduced incidence of adverse events of steroid diabetes (P = 0.041), especially in patients older than 55 years of age.ConclusionThe efficiency of half-dose prednisolone combined with RTX is not inferior to the recommended treatment regimen, and this regimen can effectively reduce the incidence of steroid-induced diabetes in patients with MCD. Moreover, we recommend a half-dose prednisolone combined with RTX treatment for elderly patients with MCD.
Ferroptosis in kidney disease: a bibliometric analysis from 2012 to 2024
Authors: Hu Yuxin , Tang Jingyi , Hong Hanzhang , Chen Yexin , Ye Beibei , Gao Ziheng , Zhu Gegongming , Wang Lin , Liu Weijing , Wang Yaoxian 
Published: January 2025

Background and aimsFerroptosis, a novel concept of programmed cell death proposed in 2012, in kidney disease, has garnered significant attention based on evidence of abnormal iron deposition and lipid peroxidation damage in the kidney. Our study aim to examine the trends and future research directions in the field of ferroptosis in kidney disease, so as to further explore the target or treatment strategy for clinical treatment of kidney disease.Material and MethodsA thorough survey using the Web of Science Core Collection, focusing on literature published between 2012 and 2024 examining the interaction between kidney disease and ferroptosis was conducted. VOSviewer, CiteSpace, and Biblioshiny were used for in-depth scientometric and visualized analyses.ResultsFrom 2012 to 2024, a total of 2,244 articles met the inclusion criteria for final analysis. The number of annual publications in this area of study showed a steady pattern at the beginning of the decade. The top 3 journals with the highest publication output were Renal Failure, Oxidative Medicine And Cellular Longevity, and Biomedicine &amp; Pharmacotherapy. China and the United States had the highest number of publications. Central South University and Guangzhou Medical University as the most active and influential institutions. Documents and citation analysis suggested that Andreas Linkermann, Jolanta Malyszko, and Alberto Ortiz are active researchers, and the research by Scott J. Dixon and Jose Pedro Friedmann Angeli, as the most cited article, are more important drivers in the development of the field. Keywords associated with glutathione, lipid peroxidation, and nitric oxide had high frequency in the early studies. In recent years, however, there has been a shift towards biomarkers, inflammation and necrosis, which indicate current and future research directions in this area.ConclusionThe global landscape of the ferroptosis research in kidney disease from 2012 to 2024 was presented. Basic research and mechanism exploration for renal fibrosis and chronic kidney disease may be a hot spot in the future.
Atypical functional connectome is associated with low reflective functioning in incarcerated adolescents
Authors: Derome Mélodie , Morosan Larisa , Heller Patrick , Debbané Martin 
Published: January 2025

IntroductionWhile functional neuroimaging studies have reported on the neural correlates of severe antisocial behaviors, such as delinquency, little is known about whole brain resting state functional connectivity (FC) of incarcerated adolescents (IA). The aim of the present study is to identify potential differences in resting state connectivity between a group of male IA, compared to community adolescents (CA). The second objective is to investigate the relations among FC and psychological factors associated with delinquent behaviors, namely psychopathic traits (callous unemotional traits, interpersonal problems, and impulsivity), socio-cognitive (empathy and reflective functioning RF) impairments and psychological problems (externalizing, internalizing, attention and thought problems).Methods31 male IA and 30 male CA participated in 8 minutes resting state functional MRI. Network Based Statistics (NBS) was used to compare FC among 142 brain regions between the two groups. Correlation and regressions analysis were performed to explore the associations between FC and the self-reported psychopathic traits, empathy, RF, and psychological problems.ResultsCompared to the CA, the IA group presented significantly increased resting state FC in a distributed subnetwork including medial prefrontal, posterior and dorsal cingulate, temporal, and occipital regions. Both within the IA group and across the whole sample, increased mean connectivity of the subnetwork correlated with lower RF (RF uncertainty). Across the whole sample, the mean connectivity was associated with higher scores of externalizing problems and impulsivity dimension of psychopathy.DiscussionWhile extending the characterization of whole brain resting state FC in IA, our results also provide insights into the neurofunctional mechanisms linking low reflective functioning abilities to externalizing behavior during adolescence.