bluuebunny/crossref_metadata_2025_split huggingface dataset:
End-of-life perspectives according to family caregivers of older adults with dementia: A qualitative study
Authors: Pires de Andrade Lage Cabral Bárbara , Martino Buttros Giulia , Leite Brito Eunice M , Giacomin Karla C , Guimarães Assis Marcella 
Published: December 2022

Background Family caregivers are primarily responsible for the care of older adults with dementia, and the demands of this care increase as the end of life approaches. Experiencing the end of a family member’s life can be stressful, and caregivers consider important to know how to identify when their loved one is approaching the end of life in order to prepare for this moment. Thus, as the family is primarily responsible for the older adult with dementia, it is essential to know the meanings of the end of life attributed by family caregivers. Objective To analyze the perceptions of family caregivers of older adults with dementia about the end of life. Methods This is a qualitative, descriptive and exploratory study. The convenience sample consisted of family caregivers of older adults with dementia. The inclusion criteria were being a family member directly involved in the care of the older adult with dementia and being 18 years of age or older. Data were collected from individual semi-structured interviews. The number of participants was defined during the interviews using the saturation criterion. Data were analyzed using the thematic analysis technique. Results A total of 63 family caregivers participated, 74.6% of whom were women, predominantly wives and daughters. Two themes resulted from the data analysis: (i) Different perspectives on the end of life: from death itself to a sad, painful and long grief process; and (ii) End of life in the perspective of transcendence. The meaning of the end of life was perceived in different ways by family caregivers. Understanding how they perceive and experience the end-of-life process of the older adult with dementia helps to clarify the best ways for the health professionals to approach and intervene with these families.
Mean distance from skin to epiglottis in parturients as measured by airway ultrasound
Authors: Neish Emma , Collins Jeremy , Sniecinski Roman M 
Published: December 2022

Introduction: There is an increasing interest in using airway ultrasound to predict difficult intubation. Studies to date have excluded pregnant women in reporting airway measurements. We performed this study to compare the mean distance from skin to epiglottis in parturients to that reported in previously published studies. We also assessed the correlation of mean distance from skin to epiglottis with other elements of the airway examination. Methods: A total of 100 parturients were recruited from a tertiary hospital’s labor and delivery floor. Standard physical examination parameters were recorded in addition to the mean distance from skin to epiglottis for all subjects. The ratio of height-to-thyromental distance was used to classify airways as potentially favorable or unfavorable. Results: The average mean distance from skin to epiglottis in parturients was 19.9 ± 3.3 mm and followed a normal distribution. The mean distance from skin to epiglottis was moderately correlated with height and body mass index. There was no difference in mean distance from skin to epiglottis between subjects with favorable versus unfavorable airways as classified by ratio of height-to-thyromental distance. Conclusion: The typical mean distance from skin to epiglottis in parturients falls between previously published values in mixed populations. Previously published cut-off values using airway ultrasound to predict difficult intubation are not likely to apply to parturients.
An Explorative Investigation of the Effect of Naturalistic Light on Agitation-Associated Behavior in Nursing Home Residents With Dementia: A Pilot Study
Authors: Saidane Hafed Amin , Rasmussen Thomas , Andersen Knud , Iversen Helle Klingenberg , West Anders Sode 
Published: December 2022

Aim: To study the effect of naturalistic light, programmed to replicate the spectrum distribution of natural light from dusk to dawn, on agitation measured with a Cohen-Mansfield Agitation Inventory–inspired score of nursing home residents with dementia. Background: Though the effects of different types of light on the sleep-wake patterns of senior adults and people with dementia have been examined in several studies, the effects of naturalistic light systems, as a possible nonpharmacological intervention to improve sleep and reduce agitation, have yet to be extensively evaluated due to the relative novelty of the technology. Methods: The study was designed as a 6-month pilot study of a prospective interventional longitudinal cohort study, with five participants recruited from a single department of a Danish nursing home. The effect of naturalistic lighting on agitation-associated behaviors was recorded over a 3-day period pre- and postintervention. Results: An overall 71.2% reduction in the frequency of agitation-associated behaviors was recorded, with the frequency of some behaviors even reduced by 100%. Conclusions: This pilot study estimates that naturalistic lighting may be a promising nonpharmacological intervention to improve the overall agitation of nursing home residents with dementia, with a possible added benefit of an improved work environment for the staff. This study, therefore, finds indication for the performance of a randomized controlled trial with the same intervention and a larger cohort.
A 15-year longitudinal analysis of the trends in the surgical management of renal and ureteric stones in Australia
Authors: Arora Bharti , Chung Eric 
Published: December 2022

Objective: This study evaluates the evolving surgical management of renal stones in Australia over the past 15 years. Methods: The National Australian Medicare and Australian Institute of Health and Welfare (AIWH) data were used to obtain the annual number of hospital presentations for urolithiasis and surgical procedures for upper urinary tract stones from 2005 to 2020. The four treatment modalities observed included nephrolithotomy, shockwave lithotripsy (SWL), percutaneous nephrolithotomy (PCNL) and endoscopic lithotripsy. Results: The total number of renal colic presentations in Australian hospitals and urology procedures claimed by Medicare has increased during the past 15 years. A total of 218,999 urological procedures were claimed by Medicare for renal stones over 15 years. There has been a dramatic rise in endoscopic lithotripsy surgery for stones. In 2005–2006, endoscopic lithotripsy comprised 56.6% of total stonework claimed by Medicare, and this proportion jumped to 88.9% in 2019–2020. All other modalities saw a decline in absolute and relative numbers. Conclusion: Endoscopic management of upper renal tract stones predominates in the current management of renal stone disease in Australia. Epidemiology audits such as this study can provide insight into national disease trends and guide future healthcare policies and resource planning. Level of evidence: 2b
Unilateral primary carcinoma of the kidney with central nervous system invasion and vertebral lysis in a cat
Authors: Ludwig Latasha , Husnik Roman , Rätsep Emily , Beeler-Marfisi Janet , Stalker Margaret , Wood Geoffrey A , Woods J Paul 
Published: December 2022

Case summary A young adult female spayed domestic shorthair cat presented for acute hindlimb weakness and anorexia with a 1-month history of lethargy, hyporexia and weight loss. A mass was palpable in the caudolateral abdomen and the left hindlimb was diffusely edematous. Abdominal ultrasound showed hydronephrosis of the left kidney with suspected hydroureter and heterogeneous tissue in the dorsal abdomen. CT evaluation confirmed a mass extending from the left kidney through the lumbar musculature with hydronephrosis, aortic attenuation, caudal vena caval thrombosis and lysis of vertebrae 4 and 5. Fine-needle aspiration of the mass suggested squamous cell carcinoma. Owing to clinical deterioration, euthanasia was elected. At necropsy, the left kidney was firmly adhered to the lumbar region with tissue that obliterated the musculature and surrounded the aorta and vena cava. There was hydronephrosis of the left kidney. Histopathologic evaluation of the mass revealed islands of neoplastic epithelial cells separated by fibrous connective tissue and areas of gradual keratinization with rare squamous metaplasia. The histologic diagnosis was invasive carcinoma with desmoplasia and vascular invasion. Relevance and novel information Primary carcinomas of the kidney in cats are rare and this report documents a progression of disease not previously reported in cats. This is the second reported case of a primary carcinoma of renal origin with features of squamous cell carcinoma in a cat, and the first with lumbar and vascular invasion. This is also the first use of kidney injury molecule-1 to help investigate tumor differentiation in cats.
Attitudes and Opinions of the Teaching Faculty Toward the LGBT Community
Authors: Gururaj G. P. , Manohar J. Shivananda , Rao T. S. Sathyanarayana 
Published: December 2022

Studies have shown physician homophobia as a barrier to health care, with LGBTQIA+ patients being more likely to delay or avoid getting care due to fear of harassment or discrimination. Objectives: Our aims and objective in this study were to determine the attitudes and opinions of the various medical and surgical teaching faculties and compare them for gender differences, if any, toward the LGBT community. Methodology: It was a cross-sectional study done among 129 teaching faculty between November and December 2019 at Sri Devaraj Urs Academy of Higher Education and Research, a rural tertiary medical college in southern India with regular and ongoing postgraduate training in all medical and surgical specialties, with assessment done using a structured and validated instrument, Riddle homophobia scale, meant for the assessment of attitudes and beliefs toward the LGBT community. Results: Most of the teachers in both the medical and surgical groups reported favorable positive attitudes/beliefs with statements like homophobia and heterosexism are wrong, and there is no need for the LGBT people to undergo reparative surgery; they deserve the same rights and privileges as everyone; however, there were negative attitudes and opinions reported with the statements like the LGBT people did not choose their sexual orientation and teachers themselves need to undergo introspection to be able to be supportive toward them, which is a significant source of concern. There were no differences in attitudes and opinions based on the age groups of the teachers of both the groups. Male teachers, when compared to female teachers (male:females—84:45), reported more favorable beliefs ( t2/ p value= 6.316/.0043) toward the LGBT community at p value ≤ .05. Conclusions: The teaching of sexuality, especially on the LGBT community, to medical professionals, especially teaching faculty, needs to be perceptive to the issues faced by people with different sexual orientations and identities.
Banking on virus-specific T cells to fulfill the need for off-the-shelf cell therapies
Authors: Quach David H. , Lulla Premal , Rooney Cliona M. 
Published: December 2022

Abstract
Adoptively transferred virus-specific T cells (VSTs) have shown remarkable safety and efficacy for the treatment of virus-associated diseases and malignancies in hematopoietic stem cell transplant (HSCT) recipients, for whom VSTs are derived from the HSCT donor. Autologous VSTs have also shown promise for the treatment of virus-driven malignancies outside the HSCT setting. In both cases, VSTs are manufactured as patient-specific products, and the time required for procurement, manufacture, and release testing precludes their use in acutely ill patients. Further, Good Manufacturing Practices–compliant products are expensive, and failures are common in virus-naive HSCT donors and patient-derived VSTs that are rendered anergic by immunosuppressive tumors. Hence, highly characterized, banked VSTs (B-VSTs) that can be used for multiple unrelated recipients are highly desirable. The major challenges facing B-VSTs result from the inevitable mismatches in the highly polymorphic and immunogenic human leukocyte antigens (HLA) that present internally processed antigens to the T-cell receptor, leading to the requirement for partial HLA matching between the B-VST and recipient. HLA mismatches lead to rapid rejection of allogeneic T-cell products and graft-versus-host disease induced by alloreactive T cells in the infusion product. Here, we summarize the clinical outcomes to date of trials of B-VSTs used for the treatment of viral infections and malignancies and their potential as a platform for chimeric antigen receptors targeting nonviral tumors. We will highlight the properties of VSTs that make them attractive off-the-shelf cell therapies, as well as the challenges that must be overcome before they can become mainstream.
Nonsteroidal anti-inflammatory drugs as a targeted therapy for bone marrow failure in Ghosal hematodiaphyseal dysplasia
Authors: Brown Timothy J. , Barrett Neil , Meng Hu , Ricciotti Emanuela , McDonnell Ciara , Dancis Andrew , Qualtieri Julianne , FitzGerald Garret A. , Cotter Melanie , Babushok Daria V. 
Published: December 2022

Abstract
Advances in genomic diagnostics hold promise for improved care of rare hematologic diseases. Here, we describe a novel targeted therapeutic approach for Ghosal hematodiaphyseal dysplasia, an autosomal recessive disease characterized by severe normocytic anemia and bone abnormalities due to loss-of-function mutations in thromboxane A synthase 1 (TBXAS1). TBXAS1 metabolizes prostaglandin H2 (PGH2), a cyclooxygenase (COX) product of arachidonic acid, into thromboxane A2. Loss-of-function mutations in TBXAS result in an increase in PGH2 availability for other PG synthases. The current treatment for Ghosal hematodiaphyseal dysplasia syndrome consists of corticosteroids. We hypothesize that nonsteroidal anti-inflammatory drugs (NSAIDs), which inhibit COX-1 and COX-2, could ameliorate the effects of TBXAS1 loss and improve hematologic function by reducing prostaglandin formation. We treated 2 patients with Ghosal hematodiaphyseal dysplasia syndrome, an adult and a child, with standard doses of NSAIDs (aspirin or ibuprofen). Both patients had rapid improvements concerning hematologic parameters and inflammatory markers without adverse events. Mass spectrometry analysis demonstrated that urinary PG metabolites were increased along with proinflammatory lipoxygenase (LOX) products 5-hydroxyeicosatetraenoic acid and leukotriene E4. Our data show that NSAIDs at standard doses surprisingly reduced both COX and LOX products, leading to the resolution of cytopenia, and should be considered for first-line treatment for Ghosal hematodiaphyseal dysplasia syndrome.
The role of Th17 cells in chronic lymphocytic leukemia: friend or foe?
Authors: Gamal Wael , Sahakian Eva , Pinilla-Ibarz Javier 
Published: December 2022

Abstract
T helper 17 (Th17) cells have a prominent role in autoimmune diseases. In contrast, the nature of these cells in cancer is controversial, with either pro- or antitumorigenic activities depending on various cancer settings. Chronic lymphocytic leukemia (CLL), a B-cell malignancy, is characterized by an imbalance in T-cell immune responses that contributes to disease progression and increased mortality. Many clinical reports indicate an increase in Th17 cells and/or interleukin 17 serum cytokine levels in patients with CLL compared with healthy individuals, which correlates with various prognostic markers and significant changes in the tumor microenvironment. The exact mechanisms by which Th17 cells might contribute to CLL progression remain poorly investigated. In this review, we provide an updated presentation of the clinical information related to the significance of Th17 cells in CLL and their interaction with the complex leukemic microenvironment, including various mediators, immune cells, and nonimmune cells. We also address the available data regarding the effects of CLL-targeted therapies on Th17 cells and the potential of using these cells in adoptive cell therapies. Having a sound understanding of the role played by Th17 cells in CLL is crucial for designing novel therapies that can achieve immune homeostasis and maximize clinical benefits.
Small molecule drug and biotech drug interaction prediction based on multi-modal representation learning
Authors: Huang Dingkai , He Hongjian , Ouyang Jiaming , Zhao Chang , Dong Xin , Xie Jiang 
Published: December 2022

Abstract
Background
Drug–drug interactions (DDIs) occur when two or more drugs are taken simultaneously or successively. Early detection of adverse drug interactions can be essential in preventing medical errors and reducing healthcare costs. Many computational methods already predict interactions between small molecule drugs (SMDs). As the number of biotechnology drugs (BioDs) increases, so makes the threat of interactions between SMDs and BioDs. However, few computational methods are available to predict their interactions.

Results
Considering the structural specificity and relational complexity of SMDs and BioDs, a novel multi-modal representation learning method called Multi-SBI is proposed to predict their interactions. First, multi-modal features are used to adequately represent the heterogeneous structure and complex relationships of SMDs and BioDs. Second, an undersampling method based on Positive-unlabeled learning (PU-sampling) is introduced to obtain negative samples with high confidence from the unlabeled data set. Finally, both learned representations of SMD and BioD are fed into DNN classifiers to predict their interaction events. In addition, we also conduct a retrospective analysis.

Conclusions
Our proposed multi-modal representation learning method can extract drug features more comprehensively in heterogeneous drugs. In addition, PU-sampling can effectively reduce the noise in the sampling procedure. Our proposed method significantly outperforms other state-of-the-art drug interaction prediction methods. In a retrospective analysis of DrugBank 5.1.0, 14 out of the 20 predictions with the highest confidence were validated in the latest version of DrugBank 5.1.8, demonstrating that Multi-SBI is a valuable tool for predicting new drug interactions through effectively extracting and learning heterogeneous drug features.
A novel glycosylation-related gene signature predicts survival in patients with lung adenocarcinoma
Authors: Liang Jin-xiao , Chen Qian , Gao Wei , Chen Da , Qian Xin-yu , Bi Jin-qiao , Lin Xing-chen , Han Bing-bing , Liu Jin-shi 
Published: December 2022

Abstract
Background
Lung adenocarcinoma (LUAD) is the most common malignant tumor that seriously affects human health. Previous studies have indicated that abnormal levels of glycosylation promote progression and poor prognosis of lung cancer. Thus, the present study aimed to explore the prognostic signature related to glycosyltransferases (GTs) for LUAD.

Methods
The gene expression profiles were obtained from The Cancer Genome Atlas (TCGA) database, and GTs were obtained from the GlycomeDB database. Differentially expressed GTs-related genes (DGTs) were identified using edge package and Venn diagram. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and ingenuity pathway analysis (IPA) methods were used to investigate the biological processes of DGTs. Subsequently, Cox and Least Absolute Shrinkage and Selection Operator (LASSO) regression analyses were performed to construct a prognostic model for LUAD. Kaplan–Meier (K–M) analysis was adopted to explore the overall survival (OS) of LUAD patients. The accuracy and specificity of the prognostic model were evaluated by receiver operating characteristic analysis (ROC). In addition, single-sample gene set enrichment analysis (ssGSEA) algorithm was used to analyze the infiltrating immune cells in the tumor environment.

Results
A total of 48 DGTs were mainly enriched in the processes of glycosylation, glycoprotein biosynthetic process, glycosphingolipid biosynthesis-lacto and neolacto series, and cell-mediated immune response. Furthermore, B3GNT3, MFNG, GYLTL1B, ALG3, and GALNT13 were screened as prognostic genes to construct a risk model for LUAD, and the LUAD patients were divided into high- and low-risk groups. K–M curve suggested that patients with a high-risk score had shorter OS than those with a low-risk score. The ROC analysis demonstrated that the risk model efficiently diagnoses LUAD. Additionally, the proportion of infiltrating aDCs (p < 0.05) and Tgds (p < 0.01) was higher in the high-risk group than in the low-risk group. Spearman’s correlation analysis manifested that the prognostic genes (MFNG and ALG3) were significantly correlated with infiltrating immune cells.

Conclusion
In summary, this study established a novel GTs-related risk model for the prognosis of LUAD patients, providing new therapeutic targets for LUAD. However, the biological role of glycosylation-related genes in LUAD needs to be explored further.
Genome-wide association study reveals the genetic determinism of serum biochemical indicators in ducks
Authors: Tang Hehe , Zhang He , Liu Dapeng , Wang Zhen , Yu Daxin , Fan Wenlei , Guo Zhanbao , Huang Wei , Hou Shuisheng , Zhou Zhengkui 
Published: December 2022

Abstract
Background
The serum is rich in nutrients and plays an essential role in electrolyte and acid–base balance, maintaining cellular homeostasis. In addition, serum parameters have been commonly used as essential biomarkers for clinical diagnosis. However, little is known about the genetic mechanism of the serum parameters in ducks.

Results
This study measured 18 serum parameters in 320 samples of the F2 segregating population generated by Mallard × Pekin duck. The phenotypic correlations showed a high correlation between LDH, HBDH, AST, and ALT (0.59–0.99), and higher coefficients were also observed among TP, ALB, HDL-C, and CHO (0.46–0.87). And then, we performed the GWAS to reveal the genetic basis of the 18 serum biochemical parameters in ducks. Fourteen candidate protein-coding genes were identified with enzyme traits (AST, ALP, LDH, HBDH), and 3 protein-coding genes were associated with metabolism and protein-related serum parameters (UA, TG). Moreover, the expression levels of the above candidate protein-coding genes in different stages of breast muscle and different tissues were analyzed. Furthermore, the genes located within the high-LD region (r2 > 0.4 and − log10(P) < 4) neighboring the significant locus also remained. Finally, 86 putative protein-coding genes were used for GO and KEGG enrichment analysis, the enzyme-linked receptor protein signaling pathway and ErbB signaling pathway deserve further focus.

Conclusions
The obtained results can contribute to new insights into blood metabolism and provide new genetic biomarkers for application in duck breeding programs.