bluuebunny/crossref_metadata_2025_split huggingface dataset:
TheAcinetobacter baumanniivirulence factor Omp33 is important for tolerance to benzalkonium chloride
Authors: Naidu Varsha , Shah Bhumika S. , Hassan Karl A. , Paulsen Ian T. 
Published: July 2023

AbstractBenzalkonium chloride (BAC) is widely used in many disinfectant solutions in clinical settings to eradicate potential bacterial pathogens, such asAcinetobacter baumannii. We sought to investigate the transcriptomic response of a drug resistantA. baumanniiisolate, AB5075-UW, on exposure to a sub-inhibitory concentration of BAC. Our transcriptomic analysis found that BAC caused an increase in the expression of genes associated with protein synthesis, such as translation initiation factors, ribosomal proteins and tRNA synthetases. It also induced the expression of genes associated with energy production and central carbon metabolism. We also observed increased expression of peptidoglycan and rod shape determining genes, which may provide increased mechanical strength to withstand osmotic challenges posed by compounds such as BAC. The most highly expressed genes under BAC stress include those that encode the RND efflux pump AdeABC and theA. baumanniiporin Omp33. Mutants ofadeABCand its regulator genesadeRShad a higher susceptibility to BAC. Disruption of the gene encoding Omp33 also resulted in higher susceptibility to BAC, and complementation of the mutant withomp33together with a 450bp upstream region restored tolerance to BAC to parental strain levels (AB5075-UW). Site directed mutagenesis of amino acids associated with Omp33 periplasmic turn (T1), which folds into the lumen of the porin and blocks the channel, suggests that Omp33 may act to prevent entry of BAC into the cell. In previous studies, Omp33 has been described as an important virulence factor inA. baumannii.The results presented in this study describe a novel role for Omp33 in BAC tolerance and reveal thatA. baumanniitolerates BAC stress through a combination of mechanisms.
Apical annuli are specialised sites of post-invasion secretion of dense granules inToxoplasma
Authors: Chelaghma Sara , Ke Huiling , Barylyuk Konstantin , Krueger Thomas , Koreny Ludek , Waller Ross F. 
Published: July 2023

ABSTRACTApicomplexans are ubiquitous intracellular parasites of animals. These parasites use a programmed sequence of secretory events to find, invade, and then reengineer their host cells to enable parasite growth and proliferation. The secretory organelles micronemes and rhoptries mediate the first steps of invasion. Both secrete their contents through the apical complex which provides an apical opening in the parasite’s elaborate inner membrane complex (IMC) — an extensive subpellicular system of flattened membrane cisternae and proteinaceous meshwork that otherwise limits access of the cytoplasm to the plasma membrane for material exchange with the cell exterior. After invasion, a second secretion programme drives host cell remodelling and occurs from dense granules. The site(s) of dense granule exocytosis, however, has been unknown. InToxoplasma gondii, small subapical annular structures that are embedded in the IMC have been observed, but the role or significance of these apical annuli to plasma membrane function has also been unknown. Here, we determined that integral membrane proteins of the plasma membrane occur specifically at these apical annular sites, that these proteins include SNARE proteins, and that the apical annuli are sites of vesicle fusion and exocytosis. Specifically, we show that dense granules require these structures for the secretion of their cargo proteins. When secretion is perturbed at the apical annuli, parasite growth is strongly impaired. The apical annuli, therefore, represent a second type of IMC-embedded structure to the apical complex that is specialised for protein secretion, and reveal that inToxoplasmathere is a physical separation of the processes of pre- and post-invasion secretion that mediate host-parasite interactions.
Structure and heterogeneity of a highly cargo-loaded encapsulin shell
Authors: Kwon Seokmu , Andreas Michael P. , Giessen Tobias W. 
Published: July 2023

AbstractEncapsulins are self-assembling protein nanocompartments able to selectively encapsulate dedicated cargo enzymes. Encapsulins are widespread across bacterial and archaeal phyla and are involved in oxidative stress resistance, iron storage, and sulfur metabolism. Encapsulin shells exhibit icosahedral geometry and consist of 60, 180, or 240 identical protein subunits. Cargo encapsulation is mediated by the specific interaction of targeting peptides or domains, found in all cargo proteins, with the interior surface of the encapsulin shell during shell self-assembly. Here, we report the 2.53 Å cryo-EM structure of a heterologously produced and highly cargo-loaded T3 encapsulin shell fromMyxococcus xanthusand explore the systems’ structural heterogeneity. We find that exceedingly high cargo loading results in the formation of substantial amounts of distorted and aberrant shells, likely caused by a combination of unfavorable steric clashes of cargo proteins and shell conformational changes. Based on our cryo-EM structure, we determine and analyze the targeting peptide-shell binding mode. We find that both ionic and hydrophobic interactions mediate targeting peptide binding. Our results will guide future attempts at rationally engineering encapsulins for biomedical and biotechnological applications.
How cell penetrating peptides behave differently from pore forming peptides: structure and stability of induced transmembrane pores
Authors: Alimohamadi Haleh , de Anda Jaime , Lee Michelle W. , Schmidt Nathan W. , Mandal Taraknath , Wong Gerard C. L. 
Published: July 2023

AbstractPeptide induced trans-membrane pore formation is commonplace in biology. Examples of transmembrane pores include pores formed by antimicrobial peptides (AMPs) and cell penetrating peptides (CPPs) in bacterial membranes and eukaryotic membranes, respectively. In general, however, transmembrane pore formation depends on peptide sequences, lipid compositions and intensive thermodynamic variables and is difficult to observe directly under realistic solution conditions, with structures that are challenging to measure directly. In contrast, the structure and phase behavior of peptide-lipid systems are relatively straightforward to map out experimentally for a broad range of conditions. Cubic phases are often observed in systems involving pore forming peptides; however, it is not clear how the structural tendency to induce negative Gaussian curvature (NGC) in such phases is quantitatively related to the geometry of biological pores. Here, we leverage the theory of anisotropic inclusions and devise a facile method to estimate transmembrane pore sizes from geometric parameters of cubic phases measured from small angle X-ray scattering (SAXS) and show that such estimates compare well with known pore sizes. Moreover, our model suggests that whereas AMPs can induce stable transmembrane pores for membranes with a broad range of conditions, pores formed by CPPs are highly labile, consistent with atomistic simulations.
Epigenetic characterization of housekeeping core promoters and their importance in tumor suppression
Authors: Loza Martin , Vandenbon Alexis , Nakai Kenta 
Published: July 2023

AbstractThere has been extensive research on describing cell type-specific (CTS) regulatory interactions, especially between enhancers and promoters. However, constitutively active interactions between CREs have been less studied. In this research, we elucidate the presence of around 11,000 housekeeping CREs (HK-CREs) and describe their main characteristics. Most of the HK-CREs are located in promoter regions, but contrary to expectations, they are not only the promoters of housekeeping genes and are involved in a broader role beyond housekeeping gene regulation. HK-CREs are conserved regions rich in unmethylated CpG sites. Their distribution across chromosomes highly correlates with that of protein-coding genes, and they interact with a large number of target genes in long-distance interactions. In the context of cancer, we observed a reduction in the activity of a subset of HK-CREs, particularly those located at the end of chromosome 19 and associated with zinc finger genes. We investigated the effect of these genes on samples from diverse cancer subtypes, observing a significant reduction in their expression due to aberrant methylation of their core promoters. Finally, an analysis of more than 5,000 patients from 17 cancer subtypes showed an increase in the survival probability of patients with higher expression of these genes, suggesting them as housekeeping tumor suppressor genes. Overall, our work unravels the presence of ubiquitously active CREs indispensable for the maintenance and stability of cells.
Design thinking in business, management and accounting: a bibliometric review and future research directions
Authors: Fatima Shaista , Singh Anurag Bhadur 
Published: July 2023

PurposeThe current study gives a quantitative analysis DT literature over the past ten years in domain of management and business where the field has witnessed a proliferation in studies. The study's primary areas of concentration were analyzing historical trends and identifying prospective future research opportunities in the field. Due of the way, it approaches innovation and problem-solving, design thinking has garnered a lot of interest from both academics and practitioners. Promoters and detractors, however, tend to have quite different perspectives on the program's core qualities, practicality and results.Design/methodology/approachThe study adopts a bibliometric approach to a systematic literature review, where the analyses and visualizations are carried out using R Studio (Biblioshiny package) and VosViewer software. The study was conducted on 518 documents extracted from the Scopus database. To identify past research trends in the field, performance analysis based on productivity and/or impact of the research constituents was carried out to understand the intellectual structure of the field.FindingsThe study's findings indicated that few areas have received the most attention, which are presented as seven themes. While the emerging themes in the field include areas such as service design, service innovation, customer experience, innovation management, project management and 21st-century skills.Practical implicationsThe studies are going deeper by breaking down concepts or processes and analyzing one aspect at a time, codesign and prototyping are such subareas within the realm of service or new product design and development.Originality/valueThis study is the first of its kind where a literature review has been conducted covering design thinking in the area of business management and accounting.
A hybrid approach to solve a raw material collecting vehicle routing problem
Authors: Tiwari Anurag , Mohapatra Priyabrata 
Published: July 2023

PurposeThe purpose of this study is to formulate a new class of vehicle routing problem with an objective to minimise the total cost of raw material collection and derive a new approach to solve optimization problems. This study can help to select the optimum number of suppliers based on cost.Design/methodology/approachTo model the raw material vehicle routing problem, a mixed integer linear programming (MILP) problem is formulated. An interesting phenomenon added to the proposed problem is that there is no compulsion to visit all suppliers. To guarantee the demand of semiconductor industry, all visited suppliers should reach a given raw material capacity requirement. To solve the proposed model, the authors developed a novel hybrid approach that is a combination of block and edge recombination approaches. To avoid bias, the authors compare the results of the proposed methodology with other known approaches, such as genetic algorithms (GAs) and ant colony optimisation (ACO).FindingsThe findings indicate that the proposed model can be useful in industries, where multiple suppliers are used. The proposed hybrid approach provides a better sequence of suppliers compared to other heuristic techniques.Research limitations/implicationsThe data used in the proposed model is generated based on previous literature. The problem derives from the assumption that semiconductor industries use a variety of raw materials.Practical implicationsThis study provides a new model and approach that can help practitioners and policymakers select suppliers based on their logistics costs.Originality/valueThis study provides two important contributions in the context of the supply chain. First, it provides a new variant of the vehicle routing problem in consideration of raw material collection; and second, it provides a new approach to solving optimisation problems.
Engaging chronically ill employees at work: the relationship between bundles of HR practices, perceived illness discrimination and work engagement
Authors: Innocenti Laura , Profili Silvia , Sammarra Alessia 
Published: July 2023

PurposeThis study aims to examine the role that four distinct bundles (developmental, utilisation, maintenance and accommodative) of HRM practices play in enhancing work engagement among chronically ill employees, and to analyse whether perceptions of discrimination on the grounds of illness can affect these relationships.Design/methodology/approachThe data were collected through a quantitative survey using a sample of 669 chronically ill employees of a major Italian company.FindingsThis study's findings confirm the importance of discerning between positive, insignificant and negative effects of distinct HR bundles on chronically ill employees' work engagement. Furthermore, this study's results suggest that the positive effect of utilisation practices (i.e. practices aimed at enabling employees to make full use of existing, but not yet necessarily utilised, individual resources) on engagement is greater when chronically ill employees perceive a discrimination-free work environment.Research limitations/implicationsThis study's findings confirm the importance of discerning between positive, no, and negative effects of distinct HR bundles on chronically ill employees' work engagement. Furthermore, this study's results suggest that the positive effect of utilisation practices (i.e. practices aimed at enabling employees to make full use of existing but not necessarily applied individual resources) on engagement is greater when chronically ill employees perceive a discrimination-free work environment.Originality/valueThe study highlights those HR bundles that have the capacity to positively affect the work engagement of chronically ill employees, a minority group rarely considered in HRM studies. Furthermore, the research identifies perceived discrimination on the grounds of illness as a contextual condition that may hinder the otherwise positive effect of HRM practices on the engagement of workers suffering from a chronic illness.
“Learn from barbarians to control barbarians”: what role has international education played in China's nation building?
Authors: Liu Wei , Huang Cen 
Published: July 2023

PurposeThe goal is to explore the role of international education in a developing country's nation building in a mostly indigenous process.Design/methodology/approachThis study reviews China's history of international education set in the larger context of different nation building tasks in the past two centuries.FindingsThe unique case of China with dramatic ups and downs in national fortunes in the past two hundred years serves to show that an open attitude to and an active engagement in international education are contributing factors for national prosperity. The case of China also serves to show that a self-determined agenda is of paramount importance in international education as a tool for nation building.Originality/valueFew studies so far have paid attention to the specific relationship between the internationalization of higher education and nation building, so the topic of the paper is an important one and a necessary addition to the existing literature. What has been the role of international education in China's national transformation? What contributions has international education made to China's achievement of nation building goals at different stages of this transformation process? What implications can other developing nations draw from China's case with regard to the role of international education in nation building? These are the questions the researchers hope to answer in this study.
Mitigating destructive deviance in organisations: assessing the role of perceived HRM practices and perceived organisational support
Authors: Malik Pooja , Malik Parul 
Published: July 2023

Purpose
The purpose of this study is to empirically examine how the seven human resource management (HRM) practises, namely, leadership development, professional investment, egalitarian practises, developmental appraisals, family-friendly practises, engagement practises and generous benefits, are associated with perceived organisational support (POS) and destructive deviance in the context of Indian organisational culture.

Design/methodology/approach
In total, 265 middle-level IT personnel provided the study's data. To test the suggested study hypotheses, confirmatory factor analysis and PROCESS Macros were used.

Findings
Results show that putting in place leadership development, professional investment, egalitarian practises, developmental appraisal, family-friendly practises, engagement practises and generous benefits generates POS that, in turn, significantly contributes to reducing destructive deviance. Additionally, POS was found to partially mediate the relationship between perceived HRM practises and destructive deviance.

Research limitations/implications
The results would help firms reduce detrimental employee deviation. To do this, managers must develop and put into effect the seven HRM practises, which demonstrate to workers that the organisation appreciates their contributions and cares about their well-being, thereby lowering disruptive deviance. In essence, IT businesses should carefully plan and implement the HRM practises they expressly want to use rather than mindlessly copying those of rival organisations. Furthermore, to adapt to the workforce's evolving needs, these HRM practises must be properly matched with both individual and organisational goals.

Originality/value
By including destructive deviance as a result of POS, this study adds to the body of research on organisational support theory. The literature on POS and negative behaviours will take a new turn with such a focus on destructive deviance. Additionally, this study encourages academics to investigate an underlying mechanism that accounts for how HRM practises affect employees' conduct. This study also adds to the sparse body of knowledge on POS for non-Western workers. The study's findings confirm that POS is a crucial concept for both Asian and Western workers.
Circular building adaptability in adaptive reuse: multiple case studies in the Netherlands
Authors: Hamida Mohammad B. , Remøy Hilde , Gruis Vincent , Jylhä Tuuli 
Published: July 2023

Purpose
The application of circular building adaptability (CBA) in adaptive reuse becomes an effective action for resource efficiency, long-lasting usability of the built environment and the sped-up transition to a circular economy (CE). This paper aims to explore to which extent CBA-related strategies are applied in adaptive reuse projects, considering enablers and obstacles.

Design/methodology/approach
A stepwise theory-practice-oriented approach was followed. Multiple-case studies of five circular adaptive reuse projects in The Netherlands were investigated, using archival research and in-depth interviews. A cross-case analysis of the findings was deductively conducted, to find and replicate common patterns.

Findings
The study revealed that configuration flexibility, product dismantlability and material reversibility were applied across the case studies, whereas functional convertibility and building maintainability were less applied. Low cost of material reuse, collaboration among team members and organisational motivation were frequently observed enabling factors. Lack of information, technical complexities, lack of circularity expertise and infeasibility of innovative circular solutions were frequently observed obstacles to applying CBA.

Practical implications
This paper provides practitioners with a set of CBA strategies that have been applied in the real world, facilitating the application of CBA in future adaptive reuse projects. Moreover, this set of strategies provides policymakers with tools for developing supportive regulations or amending existing regulations for facilitating CE through adaptive reuse.

Originality/value
This study provides empirical evidence on the application of CBA in different real-life contexts. It provides scholars and practitioners with a starting point for further developing guiding or decision-making tools for CBA in adaptive reuse.
Italy: Political Developments and Data in 2022
Authors: RUSSO LUANA , SANDRI GIULIA , SEDDONE ANTONELLA 
Published: July 2023

AbstractThe year 2022 was (again) a year of intense political change in Italy. The President of the Republic was (re)elected in January, Prime Minister Mario Draghi resigned in July, and new elections were held in September, amidst energy and economic crises, growing inflation and the Russian invasion of Ukraine. The electoral victory of the centre‐right coalition, and in particular the success of Brothers of Italy, not only marked the rise to power of a prominent European far‐right party but also led to the nomination of the first female Prime Minister of the Italian Republic. The fall of Draghi's government and the formation of the first Meloni Cabinet raised questions about Italy's relationship with EU institutions, NATO and the transatlantic partnership.
Sinomenine regulates circTRPM7‐related pathway to inhibit gastric cancer cell growth and metastasis
Authors: Yan Jingwei , Yang Jingqing , Shen Huifen , Gao Rong , Lv Shilong 
Published: July 2023

AbstractSinomenine has been found to have antitumor effects in a variety of cancers, including gastric cancer. Circular RNA (circRNA) is an important regulator of gastric cancer progression. However, it is not known whether Sinomenine mediates gastric cancer processes by regulating circRNA‐related pathways. Quantitative real‐time PCR was used to measure the expression of circTRPM7, microRNA‐145‐5p (miR‐145‐5p), and pre‐B‐cell leukemia homeobox 3 (PBX3). MTT assay, colony formation assay, EdU assay, transwell assay, wound‐healing assay, and flow cytometry were used to detect cell proliferation, migration, invasion, and apoptosis. The expression of related proteins was detected by Western blot. Mechanically, the interaction of miR‐145‐5p with circTRPM7/PBX3 was validated by dual‐luciferase reporter assay and RIP assay. Our study showed that circTRPM7 expression was reduced in Sinomenine‐treated gastric cancer cells. Moreover, overexpression of circTRPM7 upregulated the growth and metastasis of Sinomenine‐treated gastric cancer cells. CircTRPM7 could sponge miR‐145‐5p, and miR‐145‐5p reversed the effect of circTRPM7 on the growth and metastasis of Sinomenine‐treated gastric cancer cells. PBX3 was the target of miR‐145‐5p, and knockdown of PBX3 could restore the in‐miR‐145‐5p promotion effect on the malignant behavior of Sinomenine‐treated gastric cancer cells. To sum up, our data indicated that Sinomenine played an antitumor role in gastric cancer cells via circTRPM7/miR‐145‐5p/PBX3 axis.