bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum exomiR content in perimenopausal women with hot flashes-effects of hormone replacement therapy
Authors: Han Ruxue , Ge Xiaofen , Yang Xin 
Published: March 2023

Abstract
Objective
Hormone replacement therapy (HRT) is the most effective treatment for hot flushes, which is the most common symptom of the climacteric. This study aims to explored whether estrogen can relieve the hot flushes of menopause by regulating the secretion of exosomal miRNA.
Methods
For perimenopausal women with obvious symptoms of hot flashes, miRNAs in plasma exosomes were extracted and sequenced before and after HRT, miRNA expression profiles were analyzed, and target gene prediction and functional analysis of miRNAs with large differences in expression were performed.
Results
The symptoms of hot flashes improved significantly after HRT. 22 significantly differential expressed exosomal miRNAs were screened after HRT, of which 14 were down-regulated and 3 up-regulated, and 5 were newly annotated differential miRNAs. Target gene prediction and functional analysis of miRNA also suggest that it may have a certain relationship with vasomotor symptoms.
Conclusion
The types and quantity of miRNA in plasma after HRT improves the symptoms of hot flashes are different from those before treatment. More specific ways of action still need to be experimentally verified.
Ventilator management and risk of air leak syndrome for patients with COVID-19 pneumonia: a single-center, retrospective, observational study
Authors: Miyake Nodoka , Igarashi Yutaka , Nakae Ryuta , Mizobuchi Taiki , Masuno Tomohiko , Yokobori Shoji 
Published: March 2023

Abstract
Background:

Coronavirus disease 2019 (COVID-19) pneumonia is reportedly associated with air leak syndrome (ALS), including mediastinal emphysema and pneumothorax, and has a high mortality rate. In this study, we compared values obtained every minute from ventilators to clarify the relationship between ventilator management (VM) and risk of developing ALS.

Methods

This single-center, retrospective, observational study took place at a tertiary care hospital in Tokyo, Japan, over a 21-month period. Patient background, ventilator data, and outcomes were collected from adult patients with COVID-19 pneumonia on VM. Patients who developed ALS within 30 days of VM initiation (ALS group) were compared with those who did not (non-ALS group).

Results:

Of the 105 patients, 14 (13%) developed ALS. The mean positive-end expiratory pressure (PEEP) difference was 0.33 cmH2O (95% confidence interval [CI], 0.31 to 0.33), and was higher in the ALS than in the non-ALS group (9.18 ± 2.20 vs. 8.85 ± 2.63, respectively). For peak pressure, the mean difference was − 0.18 cmH2O (95% CI, -0.20 to -0.15) (20.70 ± 5.30 in ALS vs. 20.87 ± 5.65 in non-ALS group) and the mean pressure difference of -0.05 cmH2O (95% CI, -0.04 to -0.07) (12.80 ± 3.13 vs. 12.85 ± 3.55, respectively) was also higher in the non-ALS group. The difference in single ventilation volume per ideal body weight was 0.65 mL/kg (95% CI, 0.63 to 0.66) (7.83 ± 3.16 vs. 7.18 ± 2.96, respectively), and the difference in dynamic lung compliance was 8.57 mL/cmH2O (95% CI, 8.43 to 8.70) (50.32 ± 31.68 vs. 49.68 ± 15.16, respectively); both were higher in the ALS group.

Conclusions:

There was no association between higher ventilator pressures and the development of ALS. The ALS group had higher dynamic lung compliance and higher tidal volumes, which may indicate a pulmonary contribution to ALS. VM that limits tidal volume may prevent the development of ALS.
Upregulated spinal histone deacetylases induce nociceptive sensitization by inhibiting the GABA system in chronic constriction injury-induced neuropathy in rats
Authors: Wen Zhi-Hong , Chen Nan-Fu , Cheng Hao-Jung , Kuo Hsiao-Mei , Feng Chien-Wei , Yao Zhi-Kang , Chen Wu-Fu , Sung Chun-Sung 
Published: March 2023

Abstract
Neuropathic pain (NP) affects countless people worldwide, but there is no effective treatment. Histone deacetylases (HDACs) participate in epigenetic modifications, which are involved in neuropathy-induced nociceptive sensitization. Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter that can inhibit NP. HDACs regulate glutamic acid decarboxylase (GAD) 65 and the production of its downstream metabolite GABA. However, the role of HDACs and their possible cellular mechanisms in the spinal cord in neuropathy remains unclear. We found Hdac3, Hdac4, and Hdac6 gene upregulation in the lumbar spinal cord dorsal horn (SCDH) in chronic constriction injury (CCI) rats by RT-qPCR analysis. By western blotting and immunofluorescence staining, we further confirmed that the HDAC3, HDAC4, and HDAC6 proteins were significantly upregulated, and GAD65 and GABA production decreased dramatically. Intrathecal administration of panobinostat, a non-selective HDAC inhibitor, attenuated nociceptive behavior (thermal hyperalgesia and mechanical allodynia) and restored to downregulated spinal GAD65 and GABA in CCI rats. Thus, the upregulation of HDAC expression might induce nociception through GAD65 and GABA inhibition in CCI-induced neuropathy. These findings strongly suggest that HDACs regulate inhibitory neurotransmitters as a potential therapeutic strategy for an epigenetic approach to managing NP.
Comparison of fishy substances of soybean isolates treated with different deodorization methods based on headspace gas chromatography-ion mobility spectrometry
Authors: Zhang You bang , Li Run , Xiang Dong 
Published: March 2023

Abstract
Soybean isolate protein has very important advantages including high nutritional quality, good functionality and low cost. However, it contains fishy substances that tend to give unpleasant sensory experience to consumers. For this reason, the application of soybean isolate protein in food products is limited. In this study, commercially available soybean isolates were deodorized by four different methods (including 75% ethanol, gluconolactone, lactic acid precipitation washing and dry heat). GC-IMS technique, in combination with the spectral analysis, was then employed to detect fishy substances in the samples. The results of the experimental analysis showed that 75% ethanol treatment could most effectively remove the characteristic fishy substances, such as 1-octen-3-ol, 1-hexanol and 1-pentanol.In addition, the experimental result were confirmed by simulated results using a validation model. Further, we explored the mechanism of action of ethanol deodorization and found that ethanol, which is a polar solvent, may dissolve the fishy substances in soybean isolate protein and interfere with their binding to protein; as a result, they were eluted with water during the washing step. The use of ethanol not only is safer, but also can lead to the maximum deodorization effect; thus, it should be developed to become a new deodorizer of soybean products in the future.
In-depth comparison of human serous cavity resident macrophages and dendritic cells to their murine counterparts
Authors: Randolph Gwendalyn , Han Jichang , Gallerand Alexandre , Erlich Emma , Helmink Beth , Mair Iris , Li Xin , Eckhouse Shaina , Dimou Francesca , Shakhsheer Baddr , Phelps Hannah , Chan Mandy , Schilling Joel , Finlay Conor , Allen Judith , Jakubzick Claudia , Else Kathryn , Onufer Emily , Zhang Nan 
Published: March 2023

Abstract
In murine peritoneal and other serous cavities, Gata6 drives identity of the major resident cavity macrophage population, with a smaller subset of cavity macrophages dependent upon Irf4. Here we show that Gata6+ macrophages in the human peritoneum are rare, regardless of age. Instead, many aligned with CD206+LYVE1+ mouse cavity macrophages from a differentiation stage just preceding expression of Gata6. The comparatively low abundance of CD206+ converting macrophages in healthy C57BL/6J mice was sustained after high-fat diet feeding or in mice capture from the wild, suggesting that the major resident macrophages between the species share overlapping differentiation programs but differing predispositions to distinct differentiation stages. Irf4-dependent mouse serous cavity macrophages aligned closely with CD1c+CD14+CD64+ human peritoneal dendritic cells (DCs) that, in turn, resembled CD1c+CD14-CD64- peritoneal cDC2. Peritoneal DC1 and plasmacytoid DCs were rare in both species. This cross-species resource comparing mouse and human serous cavity immune cells will facilitate translational research.
Influence of silicon and growth regulator on leaf, whorl and cob damage of maize fall armyworm in vivo
Authors: Sriniva Chinnadurai , Periyakaman Chandramani , Mookiah Shanthi , Paraman Mahendran Peyandi , Raman Renuka , Ramiah Nalini 
Published: March 2023

Abstract
Microplot field experiment was conducted to study the effect of silicon sources and growth regulator against maize fall armyworm, Spodoptera frugiperda at Thoppur village of Virudhunagar district during Rabi, 2021-22. Basal application of different doses of calcium silicate and foliar application of silicic acid and potassium silicate were carried out. The results revealed that basal application of 150 kg of calcium silicate/ha + 0.2% silicic acid @ 15 DAS + 50 ppm GA @ 30 DAS was found to be effective in reducing leaf damage (42.88% per plant), whorl damage (36.05% per plot ) and cob damage (26.92% per plot) followed by treatment with 75 kg of calcium silicate/ha + 0.2% silicic acid @ 15 DAS + 50 ppm GA @ 30 DAS with leaf, whorl and cob damage of 44.74 per cent/plant, 39.24 per cent/plot and 26.92 per cent/plot respectively. Regarding yield, maximum yield of 7, 287 kgs/ha was recorded in the treatment with basal application of 150 kg of calcium silicate/ha + 0.2% SA @ 15 DAS + 50 ppm GA @ 30 DAS followed by treatment with 75 kg of calcium silicate + 0.2% SA @ 15 DAS + 50 ppm GA @ 30 DAS (7, 092 kg/ha). Hence in the present study, the basal application of calcium silicate 150 kg/ha combined with foliar application of silicic acid (0.2%) and gibberellic acid (50 ppm) at 15 and 30 DAS reduced the leaf, whorl and cob damage level of fall armyworm on maize at field condition.
Engineering Yeasts to Grow Solely on Methanol or Formic acid coupled with CO2 fixation
Authors: Guo Yuanke , Zhang Rui , Wang Jing , Qin Ruirui , Feng Jiao , Chen Kequan , Wang Xin 
Published: March 2023

Abstract
Synthetic microorganisms capable of using one-carbon compounds, such as methanol, formic acid or carbon dioxide, are of increasing interest. In this study, we converted the yeasts of Pichia pastoris and Saccharomyces cerevisiae to both synthetic methylotroph and formatotroph, allowing them to grow on methanol and formic acid alone coupled with CO2 fixation through a synthetic C1-compound assimilation pathway (MFORG pathway). This pathway consists of a methanol-formic acid oxidation module and the reductive glycine pathway. We first assembled the MFORG pathway in P. pastoris using only native enzymes, followed by overexpression of genes in the reductive glycine pathway, blocking the native methanol assimilation pathway, and compartmentalizing the methanol oxidation module. These modifications successfully redesigned the native methylotrophic yeast P. pastoris to grow on both methanol and formic acid, where higher growth rate and yield on methanol was obtained compared to the wild-type strain. We then introduced the MFORG pathway from P. pastoris into the model yeast S. cerevisiae, establishing full synthetic methylotrophy and formatotrophy in this organism. The resulting strain was able to successfully grow on methanol or formic acid alone with consumption rates of 24 mg/L*h and 15.2 mg/L*h, respectively. The CO2 fixation ability of synthetic P. pastoris and S. cerevisiae through the MFORG pathway was confirmed by 13C-tracer analysis. Finally, production of 5-aminolevulinic acid and lactic acid with methanol as the sole carbon source was demonstrated using synthetic P. pastoris and S. cerevisiae, indicating the potential of yeasts as promising hosts for biochemical production from various one-carbon compounds.
Understanding Emotions: A PoliEMO Datasetand Multi-label Classification in Indian Elections
Authors: Surolia Anuradha , Mehta Shikha , Kumaraguru Ponnurangam 
Published: March 2023

Abstract
In recent years, multi-label emotions of user tweets have beenexplored to identify user opinion, sentiment, stance, and preferences.Many studies and datasets are available to identify and analyze useremotions. Most benchmark corpora designed for user emotion analysisinclude news websites, blogs and user tweets. However, there is little exploration of political emotions in the Indian context for multi-label emotion classification problems. This paper presents the PoliEMO dataset -a novel benchmark corpus of political tweets in a multi-label setup forIndian elections, consisting of over 3.5K tweets annotated manually. Wegenerate 6,792 labels of six emotion categories: anger, insult, joy, neutral,sadness, and shameful. We utilize the PoliEMO dataset to understandemotions in a multi-label context using state-of-the-art machine learning algorithms with multi-label classifier (BR,CC, LP, MkNN with GBand LR), deep learning (CNN, LSTM and Bi-LSTM) and transformerbased method (BERT). Our experiments show Bi-LSTM outperformswith micro-averaged F1-score of 83%, macro-averaged F1-score of 78%,and accuracy 68% as compared to state-of-the-art approaches.
Phytochemical analysis, antioxidants, α-glucosidase inhibitory activity, and Toxicity Evaluation of Orthosiphon stamineus leaf extract
Authors: Ahda Mustofa , Jaswir Irwandi , Khatib Alfi , Ahmed Qamar Uddin , Nurkhasanah Nurkhasanah , Ardini Yunita Dewi , Mohamad Sharifah Nurul Akilah Syed , Anwar Muslih , Hernawan Hernawan 
Published: March 2023

Abstract
The potency of O. stamineus as a herbal candidate has been evaluated by previous studies. The goal of this study is to compare water and a 100% ethanolic extract of O. stamineus to see which one is more effective as an α-glucosidase inhibitor and antioxidant. However, these parameters are critical in the development of herbal medicines. Furthermore, the toxicity of this herb is assessed. According to this study, water extract of O. stamineus leaves has a better inhibition activity of α-glucosidase, ABTS, and DPPH, with IC50 values of approximately 43.623±0.039 µg/mL, 27.556±0.125 µg/mL, and 95.047±1.587 µg/mL, respectively. The major active compounds are fatty acid groups such as Ethyl myristate (Tr: 20.8 min); 6-(Stearoyloxy)octadecanoic acid (Tr: 20.75 min); Linoleic acid (Tr: 23.09 min); Oleic acid (Tr: 23.22 min); and phenolic groups including D-(-)-Quinic acid (Tr:1.3 min) and Caffeic acid (Tr: 5.2 min); and carboxylic acid groups and its derivate including 2-(Benzoyloxy)-3-hydroxysuccinic acid (Tr: 7.85 min) and Tuberonic acid (Tr: 9.67 min). Therefore, this study also found that the water extract of this herb is non-toxic to zebrafish embryos and has no effect on zebrafish larvae development at concentrations less than 500 g/mL.
The Ship’s Propeller Rotation Threshold in Initiating Erosion and Sedimentation on Coastal Water
Authors: Kadir Abdul , Istadi Istadi , Subagio Agus , Iskendar Iskendar , Waluyo Waluyo , Muis Abdul , Kartikasari Dewi , Sadiah Siti , Widagdo A.B , Helios M.P , Nasir Mochammad , Nurhadi Nurhadi 
Published: March 2023

Abstract
Seabed particles with low shear stress limits are prone to erosion and sedimentation, affecting the balance of aquatic ecosystems. The water flow velocity triggered by the rotation of the ship's propellers can reach the seabed and exert significant pressure on elementary particles. However, by setting the propeller rotation threshold, the process of erosion and sedimentation of the seabed can be controlled. This study determines the propeller rotation threshold of the Landing Craft Tank (LCT) ship based on the water flow velocity it produces. The water flow velocity was investigated at several variations of propeller rotation (rpm) and variations of water depth using numerical and empirical approaches. Then, scale model experiments were carried out in the laboratory. Analysis based on the general standard criteria for erosion and sedimentation shows that an LCT vessel with a propeller diameter of 1.5 meters can use a propeller rotation of 25 rpm in all water depths. The propeller rotation of 75 rpm is safe if the propeller shaft distance to the seabed is at least 1Dp (Dp is the propeller's diameter). A 120 rpm propeller rotation is safe to use at a minimum distance of 1.5Dp, and a 230 rpm propeller rotation is safe for a minimum distance of 2Dp. Threshold values vary based on the particle type and the water depth. The propeller rotation threshold criteria are essential in determining the new under-keel clearance (UKC) to realize environmentally friendly ship operations.
A Qualitative Study on an Ebola Outbreak in the Eastern Democratic Republic of the Congo: Community Perceptions and Burial Workers’ Barriers and Support Systems
Authors: Zwick Hana , Asobee Marc Salama , Milton Beaux Kennedy , Headley Jennifer , Eagle David 
Published: March 2023

Abstract
Background
Community Health Workers (CHWs) provide vital services during disease outbreaks. Appropriate burials of those who died from an infectious disease outbreak is a critical CHW function to prevent infection and disease spread. During an Ebola Virus Disease (EVD) outbreak in Beni Town, North Kivu, Democratic Republic of the Congo, we sought to understand the nature of the community response to the outbreak, the barriers burial works faced in their health work and its impact on local burial workers and other CHWs.
Methods
12 EVD burial CHWs in Beni Town completed an hour-long qualitative in-depth interview on their experiences. They were recruited from a local counseling center. Interviews were recorded, transcribed and translated into English. A team of 3 researchers identified structural and emergent themes using applied thematic analysis.
Results
Workers reported major misconceptions in the community surrounding the initiation of the outbreak. Community misconceptions were based on widespread governmental mistrust as well as a belief system that intertwines traditional and scientific understandings of the world. EVD burial workers identified violence directed at them and community misinformation as the two largest barriers to effectively carrying out their work. They named several important support systems including family and friends, personal relaxation techniques, and a local counseling center.
Conclusions
As with other disease outbreaks globally, we found that government mistrust and religious beliefs strongly impacted community perceptions of the EVD outbreak. Previous studies have demonstrated clinic-based medical personnel are often the targets of violence, our research also shows that burial workers were also targeted and exposed to extreme levels of violence in their work. Along with their ability to effectively respond to the outbreak, violence has a negative impact on their own mental wellbeing. Burial workers found group counseling sessions to be an effective tool for managing the stress associated with their work. Further developing and testing of group-based interventions for CHWs is a priority for future research.
Partners’ Experiences of Breastfeeding: A Qualitative Evaluation of an Intervention
Authors: Blixt Ingrid , Axelsson Ove , Funkquist Eva-Lotta 
Published: March 2023

Abstract

BackgroundThe World Health Organization states that women and their families need breastfeeding support from the healthcare system. However, knowledge about the most effective way to involve the partner in breastfeeding is lacking. A qualitative evaluation can provide insight and knowledge about the partner’s experiences towards a breastfeeding support intervention and thus contribute to how forthcoming breastfeeding support policies are designed. The aim of this study was to explore partners’ experiences regarding breastfeeding.MethodsAn exploratory, longitudinal and qualitative design.Partners in an intervention group (IG) and control group (CG) participated in interviews or wrote diaries during pregnancy and two months after birth. The intervention was performed in line with the Ten Steps to Successful Breastfeeding. A purposive sample was recruited from March to December 2021. Interviews and diary entries from 16 Swedish partners (8 IG and 8 CG) were analysed by content analysis, in accordance with the COREQ guidelines.ResultsPartners’ experiences can be summarised under the main category of ‘Striving to be part of the family and important that the family’s everyday life was well-functioning’. IG partners experienced that both parents were involved and cooperated in the breastfeeding process and that guidance from healthcare professionals (HCPs) helped them to feel secure. CG partners experienced that they felt excluded and that they did not receive support from HCPs.ConclusionsBoth parents need to be targeted in breastfeeding support policies to meet the support needs. Midwives at antenatal care and child healthcare nurses at the child healthcare centre have important roles to play in providing structured breastfeeding support and a breastfeeding plan. Both IG and CG partners strived to become a part of the infant’s life and to make family life work. Midwives should involve both parents in a reflective dialogue on how the partner can be involved apart from just feeding the infant.Trial registration:Registered prospectively in the Swedish database on the 06thFebruary 2020: Centre for Clinical Research Sörmland, Uppsala University, Sweden: https://www.researchweb.org/is/foudll. The project has nr. 273762. Retrospectively registered in Australian New Zealand Clinical Trials Registry on the 10thMarch 2023 (We have receive the submit number 385535, Additional files 1 and added the clinical trial nr when we receive it).
‘Single-Seed-SpeedBulks:’ a protocol that combines ‘speed breeding’ with a cost-efficient modified single-seed descent method for rapid-generation-advancement in oat (Avena sativa L.)
Authors: Kigoni Milcah , Choi Megan , Arbelaez Juan David 
Published: March 2023

Abstract
Background
In plant breeding, one of the most cost-effective and efficient ways to increase genetic gain is to reduce the breeding cycle time. In general, modern breeding methods for self-pollinated crops should strive to develop fixed lines at the lowest possible cost and in the minimum possible amount of time. Previous studies on spring oat (Avena sativa L.) showed that combining high plant density with limited soil fertility and moisture levels in a media like sand effectively decreases the time and cost of generating fixed single-seed descent lines. More recently, ‘speed breeding’, or the exposure of prolonged photoperiod regimes of 22 hours, has been shown to decrease flowering time in oat significantly. The goal of this study was to combine ‘speed breeding’ with high-density planting in a limited soil fertility media to reduce further the costs and time required to develop oat single-seed-descent lines.
Results
We grew oat plants at low density in potting-mix (control), high density in potting-mix (HD-soil), and high density in sand (HD-sand) under 16 and 22 hours of day length. We observed that oat plants grown in HD-sand and exposed to 22 hours day length reduced their flowering time by around 20 and 5 days on average compared to those grown in control conditions at 16 and 22 hours, respectively. We also observed that 85% of plants grown at high density in sandproduced a single seed when grown in bulk conditions. In contrast, only 40% of plants grown at high density in potting-mix produced a single seed.
Conclusions
Our novel protocol showed that oat plants grown in high-density bulks, using sand media and 22-hour day length reduced their flowering time by 20 days compared to control conditions and produced plants with single seeds, following closely single-seed descent assumptions while significantly reducing labor costs and greenhouse space. This methodology can be deployed in oat breeding programs to help them accelerate their rate of genetic grain for multiple traits.