bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stable maternal proteins underlie distinct transcriptome, translatome, and proteome reprogramming during mouse oocyte-to-embryo transition
Authors: Zhang Hongmei , Ji Shuyan , Zhang Ke , Chen Yuling , Ming Jia , Kong Feng , Wang Lijuan , Wang Shun , Zou Zhuoning , Xiong Zhuqing , Xu Kai , Lin Zili , Huang Bo , Liu Ling , Fan Qiang , Jin Suoqin , Deng Haiteng , Xie Wei 
Published: July 2023

AbstractBackgroundThe oocyte-to-embryo transition (OET) converts terminally differentiated gametes into a totipotent embryo and is critically controlled by maternal mRNAs and proteins, while the genome is silent until zygotic genome activation. How the transcriptome, translatome, and proteome are coordinated during this critical developmental window remains poorly understood.ResultsUtilizing a highly sensitive and quantitative mass spectrometry approach, we obtain high-quality proteome data spanning seven mouse stages, from full-grown oocyte (FGO) to blastocyst, using 100 oocytes/embryos at each stage. Integrative analyses reveal distinct proteome reprogramming compared to that of the transcriptome or translatome. FGO to 8-cell proteomes are dominated by FGO-stockpiled proteins, while the transcriptome and translatome are more dynamic. FGO-originated proteins frequently persist to blastocyst while corresponding transcripts are already downregulated or decayed. Improved concordance between protein and translation or transcription is observed for genes starting translation upon meiotic resumption, as well as those transcribed and translated only in embryos. Concordance between protein and transcription/translation is also observed for proteins with short half-lives. We built a kinetic model that predicts protein dynamics by incorporating both initial protein abundance in FGOs and translation kinetics across developmental stages.ConclusionsThrough integrative analyses of datasets generated by ultrasensitive methods, our study reveals that the proteome shows distinct dynamics compared to the translatome and transcriptome during mouse OET. We propose that the remarkably stable oocyte-originated proteome may help save resources to accommodate the demanding needs of growing embryos. This study will advance our understanding of mammalian OET and the fundamental principles governing gene expression.
“A breach in the protocol for no good reason”: a surgical antimicrobial prophylaxis experience in an Ethiopian academic medical center
Authors: Afework Veronica , Kejela Segni , Abebe Nebyou Seyoum 
Published: July 2023

Abstract
Background
An appropriately administered surgical antimicrobial prophylaxis decreases the rate of surgical site infections. Although evidence-based clinical practice guidelines have been published on surgical antimicrobial prophylaxis, the rate of adherence to the protocol and the impact of extending antimicrobial prophylaxis postoperatively is yet to be well elucidated.

Method
A total of general surgery and vascular surgery patients with clean and clean contaminated wound undergoing elective surgical procedures were included in the study. The rate of surgical antimicrobial prophylaxis utilization, the proportion of patients whom had their antimicrobial prophylaxis extended beyond 24 h and the rate of surgical site infections across groups were evaluated.

Results
The surgical antimicrobial prophylaxis utilization rate was 90.5%. Of these patients, 12.6% were unnecessarily administered with antibiotics. An “extended” antibiotics administration beyond 24 h after the surgery was found in 40.2%. Gastrointestinal and hepato-pancreatico-biliary surgery patients had 7.9-fold rate of “extended” surgical antimicrobial prophylaxis beyond 24 h, AOR 7.89 (95% CI 3.88–20.715.62, p value < 0.0001). The overall rate of surgical site infection was 15(6.8%). The “extended” regimen of prophylactic antibiotics had no effect on the rate of surgical site infections.

Conclusion
Less than half of the patients included here had surgical antimicrobial prophylaxis regimen in accordance with the existing guidelines. The most common protocol violation was noted as extension of antimicrobial prophylaxis for more than 24 h after surgery. The extension of antimicrobial prophylaxis did not decrease the rate of surgical site infections, reaffirming the evidence that prophylactic extension of surgical antimicrobial prophylaxis is unnecessary.
Using adenosine triphosphate bioluminescence level monitoring to identify bacterial reservoirs during two consecutive Enterococcus faecium and Staphylococcus capitis nosocomial infection outbreaks at a neonatal intensive care unit
Authors: Kim Ye Ji , Hong Min Yeong , Kang Hyun Mi , Yum Sook Kyung , Youn Young Ah , Lee Dong-Gun , Kang Jin Han 
Published: July 2023

Abstract
Introduction
This study aimed to assess the role of adenosine triphosphate (ATP) bioluminescence level monitoring for identifying reservoirs of the outbreak pathogen during two consecutive outbreaks caused by Enterococcus faecium and Staphylococcus capitis at a neonatal intensive care unit (NICU). The secondary aim was to evaluate the long-term sustainability of the infection control measures employed one year after the final intervention measures.

Methods
Two outbreaks occurred during a 53-day period in two disconnected subunits, A and B, that share the same attending physicians. ATP bioluminescence level monitoring, environmental cultures, and hand cultures from healthcare workers (HCW) in the NICU were performed. Pulsed-field gel electrophoresis (PFGE) typing was carried out to investigate the phylogenetic relatedness of the isolated strains.

Results
Four cases of E. faecium sepsis (patients A-8, A-7, A-9, B-8) and three cases of S. capitis sepsis (patients A-16, A-2, B-8) were diagnosed in six preterm infants over a span of 53 days. ATP levels remained high on keyboard 1 of the main station (2076 relative light unit [RLU]/100 cm2) and the keyboard of bed A-9 (4886 RLU/100 cm2). By guidance with the ATP results, environmental cultures showed that E. faecium isolated from the patients and from the main station’s keyboard 1 were genotypically indistinguishable. Two different S. capitis strains caused sepsis in three patients. A total 77.8% (n = 7/9) of S. capitis cultured from HCW's hands were genotypically indistinguishable to the strains isolated from A-2 and A-16. The remaining 22.2% (n = 2/9) were genotypically indistinguishable to patient B-8. Three interventions to decrease the risk of bacterial transmission were applied, with the final intervention including a switch of all keyboards and mice in NICU-A and B to disinfectable ones. Post-intervention prospective monitoring up to one year showed a decrease in blood culture positivity (P = 0.0019) and catheter-related blood stream infection rate (P = 0.016) before and after intervention.

Conclusion
ATP monitoring is an effective tool in identifying difficult to disinfect areas in NICUs. Non-medical devices may serve as reservoirs of pathogens causing nosocomial outbreaks, and HCWs' hands contribute to bacterial transmission in NICUs.
Occurrence and health risk assessment of antimony, arsenic, barium, cadmium, chromium, nickel, and lead in fresh fruits consumed in South Korea
Authors: Lee Jian , Hwang Insun , Park Ye-Seul , Lee Do Yup 
Published: July 2023

AbstractAlthough various fruits are consumed as fresh produce in South Korea, information on the concentrations of heavy metals in such fruits remains lacking despite the known toxic effects of the metals. Moreover, the health risks posed by seven potentially toxic metals (As, Ba, Cd, Cr, Ni, Pb, and Sb) ingested through fruit consumption have not been assessed using recent dietary data and occurrence data. Inductively coupled plasma-mass spectrometry was used to quantify these metals in 207 samples of fresh fruits mainly consumed in South Korea. The mean concentrations (mg kg−1 fresh weight) of the metals in all fruit samples were as follows: As < 0.0021, Ba 0.3675, Cd < 0.0022, Cr 0.0307, Ni 0.0815, Pb 0.0236, and Sb < 0.0021. Only Ba showed a significant negative correlation with Pb (ρ =  −0.5385) in the studied fruits at the 95% confidence level. The non-carcinogenic risk of the seven metals in terms of hazard quotients was Pb (0.0149) > As (0.0086) > Ni (0.0081) > Sb (0.0080) > Ba (0.0031) > Cd (0.0027) > Cr (0.0001), and the hazard index, which is the sum of the hazard quotients, was 0.0275 (less than 1). The carcinogenic risks of As and Pb were 4.62E − 07 and 5.05E − 07, respectively (below 1E − 04). The hazard index of seven metals and carcinogenic risks of As and Pb indicated that no health risks were associated with fruit consumption in the Korean population. However, the hazard quotient and carcinogenic risk of Pb in apples were the highest for children aged 1–2 years, indicating that continuous targeted risk monitoring in this age group is required.
Exploring the Low Force-High Velocity Domain of the Force–Velocity Relationship in Acyclic Lower-Limb Extensions
Authors: Rivière Jean Romain , Morin Jean-Benoît , Bowen Maximilien , Cross Matt R. , Messonnier Laurent A. , Samozino Pierre 
Published: July 2023

Abstract
Purpose
To compare linear and curvilinear models describing the force–velocity relationship obtained in lower-limb acyclic extensions, considering experimental data on an unprecedented range of velocity conditions.

Methods
Nine athletes performed lower-limb extensions on a leg-press ergometer, designed to provide a very broad range of force and velocity conditions. Previously inaccessible low inertial and resistive conditions were achieved by performing extensions horizontally and with assistance. Force and velocity were continuously measured over the push-off in six resistive conditions to assess individual force–velocity relationships. Goodness of fit of linear and curvilinear models (second-order polynomial function, Fenn and Marsh’s, and Hill’s equations) on force and velocity data were compared via the Akaike Information Criterion.

Results
Expressed relative to the theoretical maximal force and velocity obtained from the linear model, force and velocity data ranged from 26.6 ± 6.6 to 96.0 ± 3.6% (16–99%) and from 8.3 ± 1.9 to 76.6 ± 7.0% (5–86%), respectively. Curvilinear and linear models showed very high fit (adjusted r2 = 0.951–0.999; SEE = 17-159N). Despite curvilinear models better fitting the data, there was a ~ 99–100% chance the linear model best described the data.

Conclusion
A combination between goodness of fit, degrees of freedom and common sense (e.g., rational physiologically values) indicated linear modelling is preferable for describing the force–velocity relationship during acyclic lower-limb extensions, compared to curvilinear models. Notably, linearity appears maintained in conditions approaching theoretical maximal velocity. Using horizontal and assisted lower-limb extension to more broadly explore resistive/assistive conditions could improve reliability and accuracy of the force–velocity relationship and associated parameters.
RETRACTED ARTICLE: Acute coronary syndrome due to left main coronary trunk compression 2 months after left atrial auricle clipping: a case report
Authors: Uchida Satoshi , Takekawa Daiki , Kato Koudai , Hirota Kazuyoshi 
Published: July 2023

Abstract
Background
Left atrial auricle (LAA) clipping is a common method of preventing cardiogenic thromboembolism. However, acute coronary syndrome (ACS) has been reported as a fatal complication of LAA clipping. We describe a case of ACS 2 months after LAA clipping.

Case presentation
A 33-year-old male with atrial fibrillation was scheduled LAA clipping during aortic valve replacement for congenital aortic bicuspid valve. The surgery went smoothly with no postoperative complications, but he suddenly went into cardiac arrest 2 months later. Emergency coronary angiography and intravascular ultrasonography revealed that compression by the clip of the left main coronary trunk had caused the ACS. Percutaneous coronary intervention with stents was performed, and the clip was removed under general anesthesia.

Conclusion
Even in the remote timepoint of LAA clipping, compression of the coronary artery by the clip should be differentiated as a cause of ACS.
Assessment of the role of telemedicine in the outcome of multiple sclerosis patients
Authors: Ahmed Mohamed Abd El-khalik , Zakaria Magd Fouad , Elaziz Azza Abd Elnasser Abd , Fouad Mohamed Mahmoud , Elbokl Ahmed Mohamed , Swelam Mahmoud Saad 
Published: July 2023

Abstract
Background
Multiple sclerosis (MS) is a chronic autoimmune disease, affecting about 2.5 million people worldwide. Telemedicine is a relatively recent telecommunication tool that has multiple formats such as store-and-forward, interactive video conferencing, remote medical record access, and remote patient monitoring. Telemedicine can be used to assess individuals with MS regarding their disease process, the development and impact of new symptoms as well as inquire about health behaviors that promote effective self-management. In this study, we aimed to evaluate the effect of telemedicine on patient satisfaction, clinical outcome and financial feasibility for MS patients.

Results
Sixty MS patients from the MS unit, at Kafr Elshikh General Hospital, were recruited and divided into 2 groups; 30 in the telemedicine group and 30 in the control group. Both groups were followed up for 12 months. We found a significant difference between the telemedicine group compared to controls as it showed less severe visual symptoms (p 0.006), a smaller number of dropouts (p 0.034) and higher patient satisfaction, with no significant difference between the two groups in the number of relapses, gait, bowel and bladder, lower limb weakness.

Conclusion
Telemedicine was found to be a promising practice that can be used to promote, coordinate and adjust ongoing clinical services of MS patients.
Inclisiran adjuvant therapy to statins for the use of hypercholesterolemia: a commentary
Authors: Vohra Laiba Imran , Rizwan Kashf , Saeed Emaan , Hamza Muhammad Syed Ali , Ochani Sidhant 
Published: July 2023

Abstract
Background
Hypercholesterolemia is a lipid disorder characterized by excessively high levels of low-density lipoproteins, which encourages fat accumulation in your arteries, hence escalating the chances of heart attack and stroke. Globally, 39% of individuals experience elevated total cholesterol levels with 98.6 million DALYs (disability-adjusted life years) caused by high non-HDL cholesterol in 2019, supposedly killing 4.4 million people.

Main body
LDL cholesterol is the primary target of treatment for lowering the risk of cardiovascular events in both primary and secondary prevention. The usual drug to achieve this goal is HMG-CoA reductase inhibitors (statins), which constitute the most potent and effective class to reduce LDL cholesterol. The current treatment of choice for hypercholesterolemia is statin therapy; however, a considerable proportion of patients are unable to reach their desired low-density lipoproteins levels (LDL), while some cannot take statins at all. The regular use and possible non-adherence to long-term therapy of statins have prompted the development of novel PCSK9-targeting drugs such as inclisiran—a synthesized small interfering RNA. Inclisiran binds to the proprotein convertase subtilisin/kexin type 9 (PCSK9) mRNA causing its disintegration and hence preventing its formation. This results in reduced amounts of PCSK9 both within and outside the cells, which significantly lowers LDL levels. Multiple double-blind, placebo-controlled Osaka Emergency Information Research Intelligence Operation Network System (ORION) trials were conducted; ORION-9 was conducted on patients with familial hypercholesterolemia and LDL cholesterol levels higher than 100 mg/dl despite taking the maximum dose of statin therapy, whereas ORION-10 and ORION-11 were conducted on patients with cardiovascular disease or having its risk factors. These patients were administered Inclisiran injections on days 1, 90 (month 3), 270 (month 9), and 450 (month 15) and were followed for 540 days. The results showed decreased LDL levels by 51% compared to the placebo and further established a strong link with reduced major adverse cardiac events rates with no effect on creatinine kinase and liver function test levels. The drug’s significant side effect was reported to be an injection site reaction.

Conclusion
Inclisiran may be utilized alone or in conjunction with other lipid-lowering treatments in individuals who are unable to take statins or for whom they are contraindicated. Furthermore, its exceptional stability throughout a broad range of heat conditions makes its use well-suited for developing countries.
Dimensions of psychopathology associated with psychotic-like experiences: Findings from the network analysis in a nonclinical sample
Authors: Rejek Maksymilian , Misiak Błażej 
Published: July 2023

Abstract

Background
Psychotic-like experiences (PLEs) are associated with a variety of psychopathological symptoms. However, it remains unknown which dimensions of psychopathology are most closely related to the occurrence of PLEs. In this study, we aimed to analyze the association of PLEs with various domains of psychopathology.

Methods
A total of 1100 nonclinical adults (aged 18–35 years, 51.4% females) with a negative history of psychiatric treatment were surveyed. Assessment of psychopathology was performed using self-reports. Symptoms associated with PLEs were explored as continuous variables and based on clinically relevant thresholds using two separate network analyses.

Results
In both network analyses, PLEs were directly connected to obsessive-compulsive disorder (OCD) symptoms, manic symptoms, depressive symptoms, and attention-deficit/hyperactivity disorder (ADHD) symptoms. Anxiety symptoms were associated with PLEs only in the network based on threshold scores. Importantly, edge weight for the connection of PLEs and OCD symptoms was significantly higher compared to edge weights of all other direct connections of PLEs with psychopathology in both networks. Edge weight for the connection between PLEs and manic symptoms was significantly higher compared to edge weights for direct connections of PLEs with depressive and ADHD symptoms in the network based on continuous scores of psychopathological symptoms. Edge weights of direct connections of PLEs with depressive, anxiety, and ADHD symptoms did not differ significantly in both networks.

Conclusions
Our findings indicate that PLEs are associated with multiple domains of psychopathology. However, these phenomena are most strongly associated with OCD symptoms regardless of their severity threshold.
Preventing Future Harm: Identifying the Drivers of an Unsafe Discharge to Improve Safety on an Inpatient Oncology Service
Authors: Roberts Thomas J. , McGuire Jessica , Clark Jeffrey W. , Drutchas Alexis E. , Photopoulos Rhaea , Das Devika , Mulvey Therese M. 
Published: July 2023

Cancer Morbidity, Mortality, and Improvement Rounds is a series of articles intended to explore the unique safety risks experienced by oncology patients through the lens of quality improvement, systems and human factors engineering, and cognitive psychology. For purposes of clarity, each case focuses on a single theme, although, as is true for all medical incidents, there are almost always multiple, overlapping, contributing factors. The quality improvement paradigm used here, which focuses on root cause analyses and opportunities to improve care delivery systems, was previously outlined in this journal. This article describes the care of a young patient with aggressive breast cancer, declining performance status, and multiple hospital admissions who died shortly after being discharged home without essential medications or an adequate plan for follow-up. The patient's death due to her malignancy was unavoidable, but she had inadequate resources before her death, leading to avoidable suffering. This outcome resulted from a series of minor errors attributable to inadequate handoffs, challenges establishing realistic goals of care, and hierarchy within and between medical teams that resulted in major lapses at the time of discharge. We explore these issues and discuss how this case led to the establishment of programs designed to empower health care providers and increase engagement of outpatient oncologists at critical points of patients' disease courses.
Pigment Epithelium-Derived Factor, a Novel Adipokine, Contributes to Gestational Diabetes Mellitus
Authors: Qi Weiwei , Zhu Dan , Yin Ping , Gu Xiaoqiong , Zhao Zhen , Li Miaoxin , Dong Chang , Tang Qilong , Xie Wanting , Zhou Ti , Xia Huimin , Qiu Xiu , Yang Xia , Gao Guoquan 
Published: July 2023

Abstract

Context
Excessive insulin resistance, inadequate insulin compensation, or both could result in gestational diabetes mellitus (GDM). Levels of pigment epithelium-derived factor (PEDF), a novel adipokine that could induce insulin resistance, are high in patients with obesity and diabetes. However, the impact of PEDF in pregnancy remains unknown.

Objective
This study aimed to elucidate the role of PEDF on insulin resistance and compensatory elevation of insulin levels during normal pregnancy and in patients with GDM.

Methods
In this population-based and cohort study, logistic regression analysis was performed to determine the association of PEDF/adiponectin/leptin levels with the risk of developing GDM and to predict postpartum prediabetes. PEDF protein, PEDF transgenic mice, PEDF knockout mice, and PEDF-neutralized antibodies were used to observe changes in insulin resistance and insulin levels with pregnancy.

Results
Plasma PEDF levels were increased in normal pregnancy and higher in GDM women. Higher PEDF levels were associated with the increased risk of developing GDM and emerged as a significant independent determinant of postpartum prediabetes in GDM women. Mechanistically, in vivo and in vitro experiments revealed that PEDF induced insulin resistance by inhibiting the insulin signaling pathway.

Conclusion
In addition to insulin resistance and upregulated insulin levels in normal pregnancy and GDM, aberrant PEDF levels can serve as a “fingerprint” of metabolic abnormalities during pregnancy. Thus, PEDF is a valuable biomarker but could interfere with the time course for early diagnosis and prognosis of GDM.