bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complication and safety of ultrasound guided percutaneous nephrolithotomy in 8 025 cases in China
Authors: Li Jianxing , Xiao Bo , Hu Weiguo , Yang Bo , Chen Liang , Hu Hao , Wang Xiaofeng 
Published: September 2023

BackgroundPercutaneous nephrolithotomy (PCNL) was mostly performed with fluoroscopy and/or ultrasonography. The safety and feasibility of PCNL performed totally under ultrasound are not clearly defined. Therefore, we introduce the 9-year experience of 8 025 ultrasound guided PCNL procedures from multiple centers in China performed by the same surgeon, to evaluate the feasibility and security of this technique.MethodsFrom September 2004 to August 2013, 8 025 cases, 4 398 males (54.8%) and 3 627 females (45.2%), whose age ranged from 6 months to 85 years old, with upper urinary tract stones, underwent PCNL in our center and the supported hospitals. Puncture site selection and channel dilation were all guided using only Doppler ultrasound. Single stones were treated in 1 356 cases, there were 2 817 cases of multi stones, and 3 852 cases of staghorn calculi. The pre- and post-operative imaging data, the intraoperative findings, operation time, perioperative complications, and related parameters were recorded.ResultsAll procedures were successful. No patients died during the operation. Average operation time was 42 minutes (range 10 to 168 minutes), 4 cases converted to open surgery, and 2 patients lost the diseased kidney due to refractory bleeding in the early stage of the PCNL. Ninety-four (1.2%) patients received blood transfusions and 20 (0.25%) patients needed highly selective renal artery embolization. Fifteen (0.19%) patients had a pleural injury. 5 457 (68%) cases were completed by a single tract and 2 568 (32%) cases added more tracts. The mean stone size (longest diameter) was 2.8 cm (range 1.2 to 26.5 cm). The final stone-free rate was 85.5%. Residual stones occurred mainly in patients with renal dysfunction, medullary sponge kidney, and complete staghorn calculi with a slim calyceal neck.ConclusionsX-ray free Doppler ultrasound guided percutaneous nephrolithotomy is feasible and safe in a variety of cases of renal and/or upper ureteral stones. The probability of radiation hazard and adjacent organ injury is low. The morbidity from major complications was reduced remarkably after special training. It is worthy of wider use compared with fluoroscopy in patients with special kidneys (e.g. solitary kidney, spinal deformity, ectopic kidney) and in infants.
Diagnostic accuracy of a single qualitative immunochemical fecal occult blood test coupled with physical measurements
Authors: Wu Taiyin , Kuo Kuanliang , Wu Yifan , Lin Kuangyang 
Published: September 2023

Background
We aimed to improve the sensitivity of immunochemical fecal occult blood test (I-FOBT) to screen colorectal neoplasm among average-risk adults.

Methods
This is a diagnostic cohort study. All health examination participants receiving a single qualitative I-FOBT and a screening colonoscopy from January 2010 to June 2011 were included. Stool specimens were collected for I-FOBT before colonoscopy. Using pathology as gold standard, significant colorectal neoplasm was defined as advanced adenoma or malignancy.

Results
A total of 1 007 health examinees were identified. Fifty-five (5.5%) had borderline positive (+/-) I-FOBT, while 38 (3.8%) had positive I-FOBT. Twenty-four (2.4%) had advanced adenoma, and five (0.5%) had carcinoma. Using borderline positive I-FOBT as cutoff value, the sensitivity and specificity for significant colorectal neoplasm were 34.5% (95% confidence interval (CI) 19.9%-52.7%) and 91.5% (95% CI 89.6%-93.1%), respectively. If combined with advanced age, high blood pressure (BP), and abdominal obesity, a fulfillment of either two criteria further increased the sensitivity to 72.4% (95% CI 54.3%-85.3%) with a specificity of 68.8% (95% CI 65.8%-71.6%).

Conclusion
The sensitivity of a single qualitative I-FOBT for the detection of significant colorectal neoplasm can be increased by coupling with age, BP, and abdominal obesity.
Understanding of myofascial trigger points
Authors: Zhuang Xiaoqiang , Tan Shusheng , Huang Qiangmin 
Published: September 2023

Objective
To investigate the current practice of myofascial pain syndrome (MPS) including current epidemiology, pathology, diagnosis and treatment.

Data sources
The data analyzed in this review were mainly from relevant articles without restriction on the publication date reported in PubMed, MedSci, Google scholar. The terms “myofasial trigger points” and “myofacial pain syndrome” were used for the literature search.

Study selection
Original articles with no limitation of research design and critical reviews containing data relevant to myofascial trigger points (MTrPs) and MPS were retrieved, reviewed, analyzed and summarized.

Results
Myofascial pain syndrome (MPS) is characterized by painful taut band, referred pain, and local response twitch with a prevalence of 85% to 95% of incidence. Several factors link to the etiology of MTrPs, such as the chronic injury and overload of muscles. Other factors, such as certain nutrient and hormone insufficiency, comorbidities, and muscle imbalance may also maintain the MTrP in an active status and induce recurrent pain. The current pathology is that an extra leakage acetylcholine at the neuromuscular junction induces persistent contracture knots, relative to some hypotheses of integration, muscle spindle discharges, spinal segment sensitization, ect. MTrPs can be diagnosed and localized based on a few subjective criteria. Several approaches, including both direct and supplementary treatments, can inactivate MTrPs. Direct treatments are categorized into invasive and conservative.

Conclusion
This review provides a clear understanding of MTrP pain and introduces the most useful treatment approaches in China.
Automatic Detection of Plateau Phases in Light Curves of Variable Stars
Authors: Lavrukhina Anastasia , Malanchev Konstantin , Kornilov Matwey V. 
Published: September 2023

Abstract
Modern astronomical surveys produce millions of light curves of variable sources. These massive data sets challenge the community to create automatic light-curve processing methods for detection, classification, and characterization of variable stars. In this paper, we present a novel method for extracting the variable components of a light curve based on Otsu’s thresholding method. To validate the effectiveness of this method, we apply it to the light curves of detached eclipsing binaries and dwarf novae, sourced from Optical Gravitational Lensing Experiment catalogs.
Vitreous Magnesium Sulfate Hydrate as a Potential Mechanism for Preservation of Microbial Viability on Europa
Authors: Parker Ceth W. , Vu Tuan H. , Kim Taewoo , Johnson Paul V. 
Published: September 2023

Abstract
Europa's subsurface ocean is postulated to contain appreciable amounts of Mg2+ and

SO

4

2
−

ions, among other species. Recent laboratory experiments have shown that when solutions containing these species freeze to Europa-relevant temperatures, they can form vitreous MgSO4 hydrate, which can remain stable at these temperatures. Since vitreous phases can protect cells from physical damage that can occur during crystallization, their presence on Europa could potentially preserve entrained microorganisms from the ocean below. However, to date, it remains unclear whether such materials actually impact microbial survival. In this work, experiments were performed in which the motile nonspore-forming Antarctic isolate Pseudoalteromonas haloplanktis in solutions of 0.1 M MgSO4 were frozen to Europa surface temperatures (100 K) under conditions that resulted in the formation of either vitreous or crystalline salt hydrates. We found that cells survived in both cases, exhibiting a 3-log reduction in viable cells in the crystalline salt hydrate case while only a 1.5-log reduction in the vitreous salt hydrate case. Scanning electron microscopy accordingly showed much higher degrees of membrane lysis and cellular damage in the crystalline salt hydrate than the vitreous case. Our results demonstrate the ability of a terrestrial oceanic microorganism to survive in MgSO4 solutions frozen to Europa surface temperatures, with enhanced viability in vitreous salt-hydrate-producing conditions versus crystalline. These findings suggest that future missions should target vitrified salt-rich environments for life detection due to this potential for preserving viable microorganisms that may be present and trapped in ocean world ices.
Les circuits courts alimentaires
Authors: Raton Gwenaëlle 
Published: September 2023

Cet article illustre de façon concrète et détournée les relations villes-campagnes. Les circuits courts alimentaires sont considérés comme vertueux (réduction des intermédiaires et des distances, « locavorisme »), mais qu’en est-il vraiment de leur durabilité, compte-tenu de leur complexité logistique ? Pour le producteur, premier maillon de la chaîne, la commercialisation et le transport représentent travail et coûts supplémentaires. Les circuits courts ne sont pas moins énergivores : flux entre fermes et points de vente fragmentés en une multitude de petits volumes. L’auteur propose plusieurs formes d’organisation pour pallier à ces inconvénients : mutualiser le transport entre producteurs partenaires et promouvoir une coopération logistique territoriale, consolider le transfert de flux en le déléguant à un prestataire extérieur. L’enjeu de la logistique et le rôle des intermédiaires non commerciaux sont clé.
Oxidized Low-Density Lipoprotein Accumulation Suppresses Glycolysis and Attenuates the Macrophage Inflammatory Response by Diverting Transcription from the HIF-1α to the Nrf2 Pathway
Authors: Ting Kenneth K Y , Yu Pei , Dow Riley , Floro Eric , Ibrahim Hisham , Scipione Corey A , Hyduk Sharon J , Polenz Chanele K , Zaslaver Olga , Karmaus Peer W F , Fessler Michael B , Röst Hannes L , Ohh Michael , Tsai Sue , Winer Daniel A , Woo Minna , Rocheleau Jonathan , Jongstra-Bilen Jenny , Cybulsky Myron I 
Published: September 2023

Abstract
Lipid accumulation in macrophages (Mφs) is a hallmark of atherosclerosis, yet how lipid accumulation affects inflammatory responses through rewiring of Mφ metabolism is poorly understood. We modeled lipid accumulation in cultured wild-type mouse thioglycolate-elicited peritoneal Mφs and bone marrow–derived Mφs with conditional (Lyz2-Cre) or complete genetic deficiency of Vhl, Hif1a, Nos2, and Nfe2l2. Transfection studies employed RAW264.7 cells. Mφs were cultured for 24 h with oxidized low-density lipoprotein (oxLDL) or cholesterol and then were stimulated with LPS. Transcriptomics revealed that oxLDL accumulation in Mφs downregulated inflammatory, hypoxia, and cholesterol metabolism pathways, whereas the antioxidant pathway, fatty acid oxidation, and ABC family proteins were upregulated. Metabolomics and extracellular metabolic flux assays showed that oxLDL accumulation suppressed LPS-induced glycolysis. Intracellular lipid accumulation in Mφs impaired LPS-induced inflammation by reducing both hypoxia-inducible factor 1-α (HIF-1α) stability and transactivation capacity; thus, the phenotype was not rescued in Vhl−/− Mφs. Intracellular lipid accumulation in Mφs also enhanced LPS-induced NF erythroid 2–related factor 2 (Nrf2)–mediated antioxidative defense that destabilizes HIF-1α, and Nrf2-deficient Mφs resisted the inhibitory effects of lipid accumulation on glycolysis and inflammatory gene expression. Furthermore, oxLDL shifted NADPH consumption from HIF-1α– to Nrf2-regulated apoenzymes. Thus, we postulate that repurposing NADPH consumption from HIF-1α to Nrf2 transcriptional pathways is critical in modulating inflammatory responses in Mφs with accumulated intracellular lipid. The relevance of our in vitro models was established by comparative transcriptomic analyses, which revealed that Mφs cultured with oxLDL and stimulated with LPS shared similar inflammatory and metabolic profiles with foamy Mφs derived from the atherosclerotic mouse and human aorta.
Effect of two types of charcoal toothpaste on the enamel surface roughness of permanent teeth
Authors: Yaghini Jaber , Moghareabed Ahmad , Hatam Faezeh , Keshani Forooz 
Published: September 2023

Background:
Charcoal in the composition of some kinds of toothpaste has created concerns regarding abrasiveness and subsequent complications. Considering the popularity of charcoal toothpaste, and the manufacturers' claims that no porosity is caused by activated carbon, this study aimed to compare the effects of two charcoal kinds of toothpaste and three conventional tubes of toothpaste on enamel surface roughness of permanent primary teeth.

Materials and Methods:
This in vitro experimental study evaluated 75 teeth mounted in acrylic resin. Teeth were divided into five groups (n = 15). The primary surface roughness of teeth was measured by a profilometer. The teeth were then subjected to wear test in a V8 cross-brushing machine with Bencer and RP charcoal toothpaste, Crest 7, Colgate Optic White, and Bencer fresh mint toothpaste. After rinsing and drying specimens, their secondary surface roughness was measured. The mean changes in the roughness profile of specimens were analyzed by a one-sample Kolmogorov–Smirnov test at a 0.05 significance level.

Results:
There was no significant difference in the mean surface roughness of specimens before and after the wear test (P > 0.05). The difference in the mean wear of five types of toothpaste was not significant either (P = 0.597). The mean changes in surface roughness were 0.0685 μm for Bencer charcoal, −0.0620 μm for RP charcoal, 0.0765 μm for Crest 7, 0.1137 μm for Colgate Optic White, and 0.1052 μm for Bencer fresh mint toothpaste.

Conclusion:
Numerous kinds of toothpaste investigated in this study did not reveal any difference in terms of wear index; however, more studies are needed to evaluate the effectiveness and efficiency of these types of toothpaste.
The oncogenic role of NOTCH1 as biomarker in oral squamous cell carcinoma and oral lichen planus
Authors: Sadeghi Ensieh Sagheb , Nematpour Fatemeh Saeid , Mohtasham Nooshin , Mohajertehran Farnaz 
Published: September 2023

Background:
Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer with heterogeneous molecular pathogenesis. Oral lichen planus (OLP) is demonstrated potentially can transfer to OSCC malignant lesions. Unfortunately, there are no definitive prognostic and predictive biomarkers for the clinical management of OSCC patients. The present research is the first study that compared an oral premalignant lesion such as OLP to malignant lesions like OSCC for NOTCH1 expression levels to better understand its oncogenic or tumor suppressive role.

Materials and Methods:
In this cross-sectional study, mRNA expression of NOTCH1 was evaluated by quantitative polymerase chain reaction in 65 tissue-embedded Paraffin-Block samples, including 32 OSCC and 33 OLP. Furthermore, we collected demographic information and pathological data, including tumor stage and grade. The association between NOTCH1 and GAPDH gene expressions was determined by Chi-squared, Spearman, and Mann–Whitney tests. A P < 0.05 was considered statistically significant for all statistical analyses.

Results:
Comparison of OSCC and OLP groups showed a statistically significant difference between the quantitative expression of the NOTCH1 gene (P < 0.001). Qualitative gene expression was divided into low expression and high expression. Both study groups demonstrated a statistically significant gene expression difference (P < 0.001). There was a statistically significant difference between age and NOTCH1 expression in the OLP group (P = 0.036). There was no correlation between NOTCH1 expression and age, gender, tumor grade, and stage.

Conclusion:
Since the OSCC is a malignant lesion and the OLP showed the possible nature of malignancy transformation, we can consider the NOTCH1 as a biomarker for the assessment of the tumorigenesis process with a definition of a standard threshold for potentially malignant lesions and malignant OSCC tumors.
Aqueous extract of Protaetia brevitarsis larvae increases mTOR-mediated growth rate in zebrafish larvae
Authors: Jayasingha Jayasingha Arachchige Chathuranga Chanaka , Lee Kyoung Tae , Choi Yung Hyun , Kim Gi-Young 
Published: September 2023

Objective:
To evaluate the effects of an aqueous extract of Protaetia brevitarsis (AEPB) on the growth of zebrafish and preosteoblast MC3T3-E1 cells.

Methods:
The effects of AEPB on the linear growth and the expression of growth-related genes in zebrafish and MC3T3-E1 cells were assessed using various molecular techniques. Furthermore, the involvement of the mammalian target of rapamycin (mTOR) pathway in AEPB-induced growth was investigated by employing the mTOR inhibitor rapamycin.

Results:
AEPB administration led to a significant and dose-dependent increase in zebrafish larvae growth over time. Additionally, AEPB treatment upregulated the expression of growth hormone-1 (GH-1), insulin-like growth factor-1 (IGF-1), growth hormone receptor-1 (GHR-1), and cholecystokinin-a (CCKA) in zebrafish. Similarly, AEPB stimulated the expression and release of IGF-1 and accelerated mTOR expression in MC3T3-E1 cells. In addition, rapamycin hindered AEPB-induced linear growth in zebrafish larvae and suppressed the expression of growth-promoting genes by inhibiting mTOR activation.

Conclusions:
AEPB shows growth-promoting effects by upregulating growth-related genes and activating the mTOR signaling pathway. Further investigations are warranted to elucidate its mechanisms of action and explore its potential application in the development of growth-enhancing supplements for various purposes.
Biochemical indicators and the Peradeniya Organophosphate Poisoning scale in prediction and prognosis of organophosphorus poisoning: An observational prospective study
Authors: Jelia Shivcharan , Lal Banwari , Airan Divya 
Published: September 2023

Objective:
To study the value of some biochemical indicators and Peradeniya Organophosphate Poisoning scale in prediction and prognosis of organophosphorus poisoning.

Methods:
This was a hospital-based prospective, observational study. Various biochemical tests viz. complete blood count, random blood sugar, liver and renal function tests, creatine phosphokinase, and electrolytes were performed. Patients were assessed based on the Peradeniya Organophosphate Poisoning scale. All the patients were followed till the end point like recovery/death.

Results:
Out of the 100 patients, 72% were males and 28% were females. The majority of the patients were farmers and 21 to 30 years of age. Suicidal was the most common manner (92, 92%). Based on the Peradeniya Organophosphate Poisoning scale, 47% were mild, 34% moderate, and 19% severe. Serum creatinine, creatine phosphokinase, serum glutamic-oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase levels showed a significant correlation with severity.

Conclusions:
Some biochemical indicators such as creatine phosphokinase, alkaline phosphatase can be used as prognostic markers of organophosphorus poisoning. The Peradeniya Organophosphate Poisoning scale can be used for assessing severity of the poisoning.
Epidemiology of animal bite injuries in North of Fars province in Iran
Authors: Karami Hamed , Jafari Fatemeh , Jeihooni Ali Khani , Amiri Sanaz , Hashemifard Tahereh , Niknam Asadollah 
Published: September 2023

Objective:
To investigate the epidemiological situation of animal bites in Rostam city of Fars province.

Methods:
This is a cross-sectional study. Cases of animal bites registered in health centers of Rostam city of Fars province from the beginning of 2014 to 2020 were analyzed using the census method. The tool for collecting information was the portal of the Ministry of Health and the registration offices of people who were referred to the rabies center.

Results:
A total of 628 cases of animal bites registered in health centers of Rostam city of Fars province from the beginning of 2014 to 2020. The mean age of the injured was (31.3 ± 20.2) years. Of the total injured cases, 414 (65.9%) were male, and 491 (78.2%) lived in villages. Most of the cases were bitten by dogs (n=420, 66.8%) and the upper limb was involved in 280 (55.2%) cases. In addition, an increasing trend was observed in the incidence of animal bites from 2015 to 2020.

Conclusions:
The incidence of animal bites in Rostam City is high, and most of the cases occur in rural areas. Considering the injuries caused by animal bites, the risk of rabies transmission, and the high costs of vaccination and serum therapy, it is necessary to hold control, educational, and vaccination programs.
The Spectrum of Inherited Gray Matter Degenerative Brain Disorders (DBD) in Children: A Single-Center Study
Authors: Prabhu Ram , Saini Arushi G. , Suthar Renu , Vyas Sameer , Malhi Prahbhjot , Attri Savita , Saini Lokesh , Sahu Jitendra K. , Sankhyan Naveen 
Published: September 2023

Abstract

Objectives:
To study the clinical spectrum of inherited gray matter degenerative brain disorders (DBD) in children.

Methods:
This cross-sectional study evaluated children up to 12 y of age, diagnosed with an inherited gray matter DBD in a tertiary care pediatric hospital between July 2019 and December 2020.

Results:
A total of 314 children with progressive neuroregression were screened. Of these, 117 children with inherited gray matter DBD were included in the study. The clinic-based prevalence of DBD was 8.2%, and inherited gray matter DBD was 3.1%. The proportion of the inherited gray matter DBD was 37.3% among the overall DBD cases. Children were categorized into three groups based on the age at onset of disease: below 2 years (N = 57, 48.7%), between 2 and 5 years (N = 32, 27.3%), and between 6 and 12 years (N = 28, 23.9%). Based on the predominant cerebral structure involved, gray matter DBD were classified as cerebral gray matter disorders (53%), basal ganglia disorders (34.1%), and cerebellar disorders (12.8%). Overall, the most common disorders were Wilson disease (18%), neuronal ceroid lipofuscinosis (NCL) (17%), and neurodegeneration with brain iron accumulation (NBIA) (16%). The most common gray matter DBD in children <2 years of age were NBIA (n = 11), Rett syndrome (n = 11), and gangliosidoses (n = 10). NCL (n = 14) and ataxia telangiectasia (n = 6) were most common in the age group of 2–5 years. Wilson disease (n = 19) was the most common disorder in the age group of 6–12 years followed by NCL (n = 4) and NBIA (n = 3).

Conclusion:
Our study highlights the burden and spectrum of gray matter DBD in children. The clinic-based prevalence of DBD was 8.2%, and of inherited gray matter DBD was 3.1%. The proportion of inherited gray matter DBD was 37.3% among the overall DBD cases. Wilson disease, NCL, and NBIA are the most common gray matter DBD in children. Timely diagnosis is important for the prevention of recurrence in subsequent pregnancies.