bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exercise and prediabetes after renal transplantation: - The EXPRED-I study
Authors: Febles Raúl Morales , Miranda Domingo Marrero , Sosa Alejandro Jiménez , Rinne Ana González , Perera Coriolano Cruz , Rodríguez Ana Elena Pérez , González Alejandra Álvarez , Martín Laura Díaz , Mena Natalia Negrín , Sørensen Cristian Acosta , Tamajón Lourdes Pérez , Hernández Aurelio Rodríguez , Rinne Federico González , González Aday Dorta , Pérez Eusebio Ledesma , Delgado Alejandra González , Rodríguez Alberto Domínguez , Baute Maria Del Carmen García , Ramírez Armando Torres , Porrini Esteban 
Published: February 2023

Abstract
Background
Post-transplant diabetes mellitus (PTDM) beyond 12 months (late PTDM) is a severe complication after renal transplantation. Late PTDM develops mostly in subjects with prediabetes. Although exercise may have a potential role in preventing late PTDM, there is no previous data on the effect of exercise in patients with prediabetes.
Material and methods
we designed a 12-month exploratory study to test the capacity of exercise in reverting prediabetes in order to prevent late-PTDM. The outcome was the reversibility of prediabetes, assessed every 3 months with an oral glucose tolerance test (OGTT). The protocol included an incremental plan of aerobic and/or strength training as well as an active plan for promoting adherence (telephone calls, digital technology, and visits).
Results
the study included 27 prediabetic patients and was early interrupted due to efficacy. At the end of follow-up, 16 (60%) patients reverted to normal glucose levels at fasting and at 120 min after the OGTTs, two time higher than the 25–30% reversibility rate shown in a previous publication, p value < 0.05. 11 subjects (40%) had persistent prediabetes. Most patients needed at least one increment of exercise prescription and extra measures to achieve compliance. Compliance measures improved the adherence to the protocol in 22 patients.
Conclusions
exercise training is effective to improve glucose metabolism in renal transplant patients with prediabetes. Exercise prescription must be conducted considering both the clinical characteristics of the patients and pre-defined strategy to promote adherence. The trial registration number of the study was NCT04489043.
Magnitude and Factors Associated with Depressive Symptoms among Post-Partum Mothers Visiting Rural health center in Ethiopia, A Cross Sectional Study
Authors: Mesfin Abigael Abiy , Yimer Abdurahman , Begashaw Abraham , Nigusu Amir , Assefa Amanuel , Zewde Amesiyas , Mesfin Samuel , Genetu Abraham 
Published: February 2023

Abstract
Background
Due to their propensity for pregnancy, childrearing, and caring for others, women are twice more likely than males to develop depression during their lifetime. Social stresses like poverty, intimate partner abuse, a history of miscarriage, and unwanted pregnancy are risk factors for postpartum depression, and these factors have a negative impact on maternal health. The mother and her children may suffer long-term negative effects if postpartum depression is left untreated. This research aims to address the gap in studies in Oromia region and contribute to building strong and a more representative evidence for postpartum depression in Ethiopia.
Methods
The study is a health facility-based cross-sectional study, among postnatal mothers visiting Batu health center. Descriptive and analytical cross-sectional study design was used among postnatal women who have given birth within the past 12 months. The Edinburgh postpartum depression scale was used to assess postpartum depression. Social support was assessed using the maternal social support scale. Chi-square test analysis was used to determine the association of post-partum depressive symptoms with socio-demographic, obstetric and psychosocial factors.
Results
Based on the cut-off points of Edinburgh Postnatal Depression Scale (≥11), 24.6 percent of the total respondents had post-partum depression whereas 75.4 percent did not have depressive symptoms. Among the mothers who had postpartum depression, majority (85.4%) had not even heard about the disease before. Among those mothers who had postpartum depression, 25% had low social support. Significant association was found between abortion history, unplanned pregnancy, history of mental illness, family history of mental illness, social support and PPD.
Conclusions
Despite the high magnitude of postpartum depression, measures to help depressed mothers are not brought to action. This highlights the need to advocate for postpartum depression services. Although the mothers with postpartum depression have made it to the health center, our study showed that they go back unnoticed. Postpartum depression screening is a simple but profound step that can be implemented. Moreover, antenatal care visits can be used as an opportunity to give health education on postpartum depression.
Satisfaction rates and preferences of men using testosterone replacement therapy.
Authors: Miller Jake , Loeb Charles , Miller Jake , Schneider Douglas , Grimaud Logan , Hammad Muhammed Moukhtar , Yafi Faysal 
Published: February 2023

Abstract
Despite a well-documented increase in both the prevalence of Testosterone Deficiency (TD) and prescription of testosterone replacement therapy (TRT), few studies have investigated the preferences of patients receiving TRT and factors associated with increased treatment satisfaction. To the preferences of patients receiving TRT and factors associated with improved treatment satisfaction, a survey was completed by 140 men receiving TRT at a single institution. Survey questions investigated demographics, symptom burden of TD, TRT regimen, treatment preferences, and treatment satisfaction. 62.7% of patients were satisfied with their current TRT regimen. Those using auto-injectors (91.7%, odds ration [OR] = 9.3), subcutaneous pellets (90.0%, OR = 15.2), and intramuscular injections (67.5%, OR = 5.7), were with significantly increased satisfaction rates (p < 0.05). The majority of patients indicated that they would prefer to receive TRT injections when self-administered or administered at home. While patients noted that treatment efficacy was a significant driving factor when evaluating a TRT regimen, few patients felt that cost was the most significant factor.
Management of severe cerebral venous sinus thrombosis after post-vaccination breakthrough COVID-19 infection: a case report and review of the literature
Authors: Giammello Fabrizio , Grillo Francesco , Tessitore Agostino , Gardin Anna , Iatì Domenico , Caragliano Antonio Armando , Velo Mariano , Buonomo Orazio , Ferraù Ludovica , Toscano Antonio , Spina Paolino La , Vinci Sergio Lucio 
Published: February 2023

Abstract
Introduction: Neurological manifestations related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may involve both peripheral and central nervous system, including acute ischemic stroke and cerebral venous sinus thrombosis (CVST).
Case Presentation: Hereby, we present an adult patient with post-vaccination breakthrough COVID-19 infection and CVST, treated with venous mechanical thrombectomy (MT). The patient manifested continuous tonic-clonic seizures, after an insidious presentation with headache and left-sided weakness. SARS-CoV-2 testing was positive, despite fully vaccination, using two approved mRNA platforms. Factor V Leiden polymorphism was detected. The patient was initially managed with anticoagulation, followed by MT with a positive response. We provided a comparison to similar cases of COVID-19 associated CVST undergoing mechanical thrombectomy.
Conclusions: High index of suspicion and prompt diagnosis are extremely important to ensure immediate hospitalization and therapy, since CVST associated with either vaccines or COVID-19 seems to evolve rapidly and with a high mortality rate. Even a breakthrough infection may present severe vascular complications. In addition, evaluation of acquired and hereditary thrombophilia may be beneficial in acute phase, also without a previous history of thrombosis. Clinicians should start early medical treatment, and additionally consider the endovascular approach as an optimistic choice in refractory CVST related to SARS-CoV-2 infection.
Impact of physical activity as an adjuvant treatment in the healing of venous ulcers in Primary Care. RCT protocol study. "Active Legs".
Authors: Herraiz-Ahijado Borja , Folguera-Álvarez Carmen , Soriano José Verdú , Vara Pilar Mori , Rico-Blázquez Milagros 
Published: February 2023

Abstract
Background: Venous ulcers usually present a torpid evolution with a negative impact on patients' quality of life. In primary care, they account for 2.5% of nursing consultations and their treatment represents high costs for national health systems. These patients usually have a low level of physical activity, with muscle pump dysfunction of the lower limbs, which may improve with increased physical activity. The purpose of this study is to analyse the effectiveness of a structured intervention involving physical activity and exercise (Active Legs) as an adjuvant treatment in improving healing of chronic venous ulcers at 3 months follow-up.
Methods:A randomized, multicentre clinical trial. A total of 224 individuals receiving primary nursing care with a diagnosis of venous ulcer, with a diameter of 1 cm or greater and an ankle-brachial index between 0.8 and 1.3, able to comply with the study requirements and consenting to participate, will be sequentially included (112 per group).
Both groups will receive the standard treatment in primary care, with cleansing, debridement and healing in a moist environment together with multilayer compression therapy. The intervention group will also receive a structured educational intervention involving lower limb physical exercise and daily ambulation guidelines.
The primary response variables will be complete healing –understood as complete and sustained epithelialisation for at least 2 weeks– and time to healing. The secondary variables will be degree of healing, ulcer area, quality of life, pain and variables related to the healing process, prognosis, and recurrences. Sociodemographic variables, adherence to treatment and satisfaction variables will also be recorded. Data will be collected at baseline, at 3 months and at 6 months follow-up. Survival analysis (Kaplan-Meier and Cox) will be performed to measure primary effectiveness. Intention-to-treat analysis.
Discussion: If the intervention is effective, a cost-effectiveness analysis could be conducted and implemented as an additional intervention in the usual venous ulcer treatment in primary care.
Trial registration: NCT04039789. [https://ClinicalTrials.gov]. 07/11/2019.
Should adjustable gastric band be abandoned as a bariatric surgery option? Private center’s experience with adjustable gastric band conversion.
Authors: Almayouf Mohammad , Alqahtani Awadh 
Published: February 2023

Abstract
Background:
The literature is lacking experience of revising adjustable gastric band at private centers in Saudi Arabia.
Methods:
A retrospective review at a private hospital was conducted. Primary endpoints were weight loss after 1 year, operative time, and complications. Conversion patients were matched with similar patient subjected to primary BS in a 1:2 ratio.
Results:
Twenty six patients were managed in a single stage conversion of AGB to laparoscopic sleeve gastrectomy (LSG). One third of the patients had weight regain (WR) and band intolerance (BI). Patients converted to LSG had mean preoperative weight and BMI of 104.9 ± 23.7 kg and 39.2 ± 5.8 kg/m2, respectively. One-year percentage of total weight loss (%TWL) and percentage of excess weight loss (%EWL) were 26.1 ± 9.6 and 76.9 ± 29.7, respectively. One patient (3.3%) developed staple-line leak and needed readmission. After 1 year, primary LSG had better weight loss compared to conversional LSG.
Conclusion:
Most patients with AGB end up with conversion mainly due to WR and BI. Conversion of AGB to other BS is safe and effective at private centers in Saudi Arabia. Weight loss after conversional LSG is inferior to primary LSG.
Functioning of local strains of bacteria under heavy metal stress (Uzbekistan)
Authors: Usmonkulovaa Aziza , Kadirova Gulchekhra , Shukurov Nosir 
Published: February 2023

Abstract
Cadmium (Cd) and nickel (Ni) are among the most toxic metals and high levels of Cd and Ni in soil have been observed in several studies to slow down plant growth, mainly biomass, chlorophyll content and photosynthetic properties. Beneficial soil bacteria are promising targets for reducing Cd and Ni toxicity and increasing plant tolerance to heavy metal stress. Different strains of bacteria were isolated from soil contaminated with heavy metals. These isolates were identified at the species level and investigated for their involvement in mitigating the deleterious effects of heavy metal stress. In this study, the minimum inhibitory concentration, biosorption of bacteria with high resistance to heavy metals to Ni(II) and Cd(II), and stimulating properties of plant productivity under conditions of heavy metal stress were studied. Isolates 5, 11, 18 and 10, 16, 11, 8 was determined against high viability to concentrations of Ni (II) and Cd (II) cations. These isolates were identified as Enterobacter cloacae (5), Enterobacter ludwigii (11), Pseudomonas aeruginosa (18), Bacillus licheniformis (10), Bacillus megaterium (16) and Bacillus simplex (8) by morphological-cultural and biochemical identification. Growth and multiplication of Bacillus megaterium (16), B. licheniformis (10) and B.subtilis (13) strains at 1–3 mM concentration of CdEnterobacter ludwigii (11) was the most effective in reducing Cd and Ni, reaching values of 36% and 41%, respectively. Pseudomonas aeruginosa (18) synthesized auxin 4, 4.8, 5.3 times higher amount compared to control and Enterobacter ludwigii (11), Bacillus licheniformis (10), Bacillus simplex (8) synthesized auxin in equal amount compared to the control at different concentrations of Cd cation (2.4, 4.1, 8.2 mg/l). Pseudomonas aeruginosa (18) strain produced 78 mg/l EPS at the concentration of 24.6 mg/l Cd cation on the 7th day of cultivation. Using microorganisms tolerant of high concentrations of heavy metals and their activity, bioremediation of ambience contaminated with heavy metals is possible. Their reduction of Ni and Cd cations, increased synthesis of phytohormones and exopolysaccharides under heavy metal stress conditions indicate that these microorganisms are promising objects in the future.
A Rare Case of Coccidioidomycosis of the Thyroid Gland
Authors: Solis-Pazmino Paola , Merchant Natalie , Padilla Osvaldo , Alkhalili Eyas 
Published: February 2023

Abstract
Infection of the thyroid gland with Coccidioides is rare, with fewer than 50 cases reported since 1970. Disseminated infection complicates less than one percent of cases and occurs most often in immunocompromised patients. Since most patients are asymptomatic, they may experience a delay in diagnosis and treatment. aspiration thyroid biopsy proved helpful in establishing the diagnosis of coccidioidomycosis. The ideal management is unclear, but some studies suggest three years of oral azoles after disseminated coccidioidomycosis to other soft tissue organs.
We report a case of disseminated coccidioidomycosis involving the thyroid gland, a sporadic infection. The overall mortality is high due to delayed diagnosis and treatment. The diagnosis is determined by the culture of a - aspirate or/and a biopsy followed by direct microscopy. However, the optimal treatment, duration, and medication dosage remain controversial.
Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis
Authors: Liu Jiasheng , Zhu Mengchao , Xu Yitong , Zhang Mengdi , Sun Haisen , Wang Yaqi , Yang Qingwen , Li Jin 
Published: February 2023

Abstract
Background:Autophagy is an important process that maintains the quality of intracellular proteins and organelles. There is extensive evidence that autophagy has an important role in the lens. Human lens epithelial cells (HLECs) play a key role in the internal homeostasis of the lens. HLEC subtypes have been identified, but autophagy-prominent cell clusters among HLECs have not been characterized.
Purpose: To explore the existence of autophagy-prominent cell clusters in HLECs.
Methods: Three donated lenses (HLECs from two whole lenses and HLECs from 1 lens without the anterior central 6-mm zone) were used for single-cell RNA sequencing (scRNA-seq). AUCell and AddModuleScore analysis were used to identify potential autophagy-prominent cell clusters. Transmission electron microscopy (TEM) was used to confirm the results.
Results: High-quality transcripts from 18,120 cells were acquired by scRNA-seq of the two intact lenses. Unsupervised clustering classified the cells into four clusters. AUCell and AddModuleScore analysis revealed the presence of autophagy-prominent cell cluster 1. scRNA-seq analysis of HLECs from the lens lacking the central zone revealed minimal expression of C8orf4, a specific marker of cell cluster 1; moreover, these HLECs exhibited less autophagy. TEM further supported the presence of an autophagy-prominent cell cluster and showed that it was located in the central zone of the lens.
Conclusions: We identified an autophagy-prominent cell cluster among HLECs and revealed that it was localized in the central zone of the lens. Our findings will aid investigations of autophagy in HLECs and provide insights to guide related research.
Acid Rock Drainage in Pyrite-rich Rock Formation at the Subsurface
Authors: Karikari-Yeboah Ohene 
Published: February 2023

Abstract
Acid Rock Drainage (ARD) is an environmental hazard associated with pyrite oxidation in rock excavations and excavation spoils. The oxidation process is initiated when pyrite, in the form of dust and fine crystals, is exposed to aqueous oxygen to release low pH acidic metal-laden leachate. The reaction is exothermic, and the heat generated is known to cause spontaneous combustion in open pit coal mines. Until recently, pyrite oxidation was considered as purely an aerobic process with the presence of aqueous oxygen as a necessary pre-condition. However, recent studies have demonstrated that the reaction can proceed in anoxic environments in the presence of an effective oxidant besides oxygen. Rock blasting in quarry and open cut mines has the tendency to induce fracturing of rock mass to significant depths below the ground surface. These fractures open the subsurface to atmospheric oxygen intrusions and runoff infiltrations. In a pyrite-rich rock mass, this creates conducive environment for pyrite oxidation and acidic leachate into the groundwater. The lower pH conditions that results are conducive for the solubilization of ferric oxides and hydroxides and the release of ferric ions in the groundwater. Ferric ions are highly soluble at low pH conditions, typically below pH 3, but precipitates out of solution when the pH rises above 3.0. In a fractured rock mass, runoff infiltrations would cause the water table and the groundwater pH to rise and dissolved ferric ions to precipitate out of solution. The rise of the water table into the fractured zone would be accompanied by groundwater seepage and the deposition of ferric ion precipitates as orange/yellow coloration along seepage paths. In this study, the extent of blasting-related rock mass fractures has been quantified by rock engineering principles. The results are linked to the origin and causes of pyrite oxidation related ARD environmental hazard in an abandoned quarry pit. The study outcome shows that the subsurface is equally susceptible to pyrite oxidation related environmental hazards with potential adverse consequences on rivers and streams.
The comprehensive analysis identifies the lncRNA involved in N6- methyladenosine and glycolysis activity in colon cancer
Authors: Luo Yi , Xie Yajun , Wu Dejun , Wang Bingyi , Lu Helei , Wang Zhiqiang , Quan Yingjun , Han BO 
Published: February 2023

Abstract
Background: N6-methyladenosine (m6A) exerted an important role in colon cancer. Nevertheless, the lncRNAs involved in this process remain unclear.
Methods: The open-accessed data used for analysis was downloaded from The Cancer Genome Atlas (TCGA) database and analyzed using the R software.
Results: In our study, we firstly collected the expression value of m6A regulators from TCGA data. Then, the lncRNAs with |Cor| > 0.3 and P < 0.05 of these m6A molecules were identified and defined as m6A-related lncRNAs. Moreover, we established a prognosis signature based on ten m6A-related lncRNAs, consisting of AL360181.1, PCAT6, SNHG26, AC016876.1, AC104667.2, AL114730.3, LINC02257, AC147067.1, AP006621.3 and AC009237.14, which showed satisfactory prediction efficiency in patients survival. Immune-related analysis indicated a diverse immune cell infiltration pattern between high and low risk patients. Also, we found that the low risk patients might be more sensitive to immunotherapy. Biological enrichment analysis indicated that the high risk patients had a higher activity of multiple carcinogenic pathways, including glycolysis. The lncRNA AL360181.1 has not been reported previously. Meanwhile, the lncRNA AL360181.1 was correlated with glycolysis activity and worse survival performance, therefore selected for further analysis. The result indicated that AL360181.1 was correlated with worse clinical features. Immune infiltration analysis indicated that AL360181.1 was positively correlated with Tcm infiltration, yet negatively correlated with Th2 cells, T cells, neutrophils, iDC and macrophages. Biological enrichment analysis indicated that the pathways of WNT/β-catenin, pancreas beta cells, hedgehog signaling, bile acid metabolism, bile acid metabolism, and fatty acid metabolism were upregulated in the patients with high AL360181.1 expression
Conclusions: Our result can improve the understanding of m6A-related lncRNA in colon cancer and provide direction for future studies.
Clinical Features and Genomic Landscape of Myeloproliferative Neoplasm (MPN) Patients with Autoimmune and Inflammatory Diseases (AID)
Authors: Elessa Dikelele , Zhao Lin-Pierre , de Oliveira Rafael Daltro , Maslah Nabih , Soret Juliette , VERGER Emmanuelle , Marcault Clémence , Parquet Nathalie , Fenaux Pierre , Adès Lionel , Raffoux Emmanuel , GIRAUDIER Stéphane , Fain Olivier , Cassinat Bruno , Kiladjian Jean-Jacques , MEKINIAN Arsène , Benajiba Lina 
Published: February 2023

Abstract
There are few data regarding the association of autoimmune and inflammatory diseases (AID) with Philadelphia negative myeloproliferative neoplasms (MPN). In this retrospective study, we describe the prevalence, clinical and biological features and outcome of AID association in MPN.
A total of 1541 MPN patients were included, encompassing 95 (6%) patients with AID. Female patients were predominant within the AID group (65% versus 54%, p=0.03). A total of 103 AID diagnoses were reported in 95 patients, including 48 organ-specific AID, 13 inflammatory arthritis, 9 connective tissue diseases, 9 dermatosis, 6 systemic vasculitis and 18 unclassified AID. The prevalence of TET2 mutations was higher in the AID cohort (32% versus 22%), although not statistically significant (p=0.08). In subgroup analysis of patients with myelofibrosis, TET2 mutations were more prevalent in AID group (p=0.025). The prevalence of driver and other additional mutations did not differ between the 2 groups. The association with AID did not impact overall survival (p=0.67), transformation-free survival (p=0.37) or secondary myelofibrosis-free survival (p=0.91).
Our data suggest that the prevalence of AID is similar in MPN patients to that of the general population. TET2 mutations are highly prevalent in MPN patients with AID potentially suggesting a shared physiopathology.