bluuebunny/crossref_metadata_2025_split huggingface dataset:
A randomized trial of once daily versus twice daily dosing of oral iron in CKD
Authors: Sood Vivek , Kamboj Kajal , Bhatia Prateek , Sharma Vishal , Kundu Monica , Ghosh Arpita , Singh Sanjay Kumar , Sen Thakur , Kaur Prabhjot , Ramachandran Raja , Rathi Manish , Kohli Harbir Singh , Gupta Krishan Lal , Malhotra Samir , Yadav Ashok Kumar , Kumar Vivek , Jha Vivekanand 
Published: January 2023

AbstractWe investigated the effect of two dosing regimens of oral iron on iron status and hematological parameters in patients with CKD. In this single center, open label, randomized, active controlled clinical trial, stable adult patients with CKD stage G3–4 with percentage transferrin saturation (%TSAT) ≤ 30% and serum ferritin ≤ 500 ng/ml were eligible. Participants were randomized to receive either 100 mg of ferrous ascorbate once daily (OD group) or 100 mg of ferrous ascorbate twice daily (BD group, total daily dose 200 mg). The primary outcome was change in %TSAT between groups over 12 weeks. The secondary outcomes were changes in other iron status and hematological parameters, serum interleukin-6 (IL-6) and hepcidin. 80 participants were enrolled out of which 76 completed the study. Change in %TSAT was not significantly different between groups (β = − 1.43, 95% CI − 3.99 to 1.12, BD group as reference). The rise in serum ferritin was less in the OD group as compared to BD group (β = − 0.36, 95% CI − 0.61 to − 0.10) whereas MCHC increased in the OD group as compared to decrease in the BD group (β = 0.37, 95% CI 0.067–0.67). These observations need exploration to ascertain the impact of different oral iron dosing strategies in CKD.
Molecular evidence of hookworms in public environment of Bangladesh
Authors: Nath Tilak Chandra , Eom Keeseon S. , Choe Seongjun , Park Hansol , Lee Dongmin 
Published: January 2023

AbstractAccurate diagnosis by precise identification of causative agents is essential for the effectiveness of any control interventions. Despite high zoonotic potential, available literature on hookworms in Bangladesh is still scarce and nonspecific. The objective of this study was to determine the occurrence of hookworms in public locations across northeastern Bangladesh (Sylhet metropolitan area) using integrated parasitological and molecular assays. A total of 130 samples (80 soil and 50 environmental canine feces) were collected and examined using modified flotation technique and formalin-ether sedimentation methods. Modified plate culture was used to isolate larvae. The identification was made based on morphometric features and confirmed by amplifying the ITS region of the nuclear rDNA. Overall, 66.2% (86/130) of examined samples were positive for hookworms infection. Characteristic eggs (61–68 × 29–37 μm) and/or larvae of hookworms were observed in 73.8% (59/80) soils and 54.0% (27/50) environmental fecal samples. Rhabditiform larvae (0.48–0.54 × 0.04–0.07 mm) were observed in cultured samples. Genetic analysis of rDNA sequences revealed the presence of Ancylostoma caninum and Ancylostoma ceylanicum. In this study, hookworms' contamination of the public environment was substantial. To the best of our knowledge, this is the first molecular proof of A. caninum and A. ceylanicum observed in urban public environment in Bangladesh.
Quantitative estimation of closed cell porosity in low density ceramic composites using X-ray microtomography
Authors: Smith J. D. , Garcia C. , Rodriguez J. , Scharf T. W. 
Published: January 2023

AbstractX-ray Microtomography is a proven tool for phase fraction analysis of multi-phase systems, provided that each phase is adequately partitioned by some means of data processing. For porosity in materials containing low-density ceramic phases, differentiation between pores and the low-density phase(s) can be intractable due to low scattering in the low-density phase, particularly if small pores necessitate low binning. We present a novel, combined methodology for accurate porosity analysis—despite these shortcomings. A 3-stage process is proposed, consisting of (1) Signal/noise enhancement using non-local means denoising, (2) Phase segmentation using a convolutional neural network, and (3) Quantitative analysis of the resulting 3D pore metrics. This particular combination of denoising and segmentation is robust against the fragmentation of common segmentation algorithms, while avoiding the volitional aspects of model selection associated with histogram fitting. We discuss the procedure applied to ternary phase SiC–TiC-diamond composites produced by reactive spark plasma sintering with porosity spanning 2–9 vol%.
On the contribution of work or heat in exchanged energy via interaction in open bipartite quantum systems
Authors: Ahmadi B. , Salimi S. , Khorashad A. S. 
Published: January 2023

AbstractThe question of with what we associate work and heat in a quantum thermodynamic process has been extensively discussed, mostly for systems with time-dependent Hamiltonians. In this paper, we aim to investigate the energy exchanged between two quantum systems through interaction where the Hamiltonian of the system is time-independent. An entropy-based re-definitions of heat and work are presented for these quantum thermodynamic systems therefore an entropy-based formalism of both the first and the second laws of thermodynamics are introduced. We will use the genuine reasoning based on which Clausius originally defined work and heat. The change in the energy which is accompanied by a change in the entropy is identified as heat, while any change in the energy which does not lead to a change in the entropy is known as work. It will be seen that quantum coherence does not allow all the energy exchanged between two quantum systems to be only of the heat form. Several examples will also be discussed. Finally, we will examine irreversibility from our entropy-based formalism of quantum thermodynamics.
Classification of static postures with wearable sensors mounted on loose clothing
Authors: Jayasinghe Udeni , Janko Balazs , Hwang Faustina , Harwin William S. 
Published: January 2023

AbstractInertial Measurement Units (IMUs) are a potential way to monitor the mobility of people outside clinical or laboratory settings at an acceptable cost. To increase accuracy, multiple IMUs can be used. By embedding multiple sensors into everyday clothing, it is possible to simplify having to put on individual sensors, ensuring sensors are correctly located and oriented. This research demonstrates how clothing-mounted IMU readings can be used to identify 4 common postures: standing, sitting, lying down and sitting on the floor. Data were collected from 5 healthy adults, with each providing 1–4 days of data with approximately 5 h each day. Each day, participants performed a fixed set of activities that were video-recorded to provide a ground truth. This is an analysis of accelerometry data from 3 sensors incorporated into right trouser-leg at the waist, thigh and ankle. Data were classified as static/ dynamic activities using a K-nearest neighbour (KNN) algorithm. For static activities, the inclination angles of the three sensors were estimated and used to train a second KNN classifier. For this highly-selected dataset (60000–70000 data points/posture), the static postures were classified with 100% accuracy, illustrating the potential for clothing-mounted sensors to be used in posture classification.
High throughput sequencing revealed enhanced cell cycle signaling in SLE patients
Authors: Yang Mingyue , Wang Peisong , Liu Tao , Zou Xiaojuan , Xia Ying , Li Chenxu , Wang Xiaosong 
Published: January 2023

AbstractThe multi-system involvement and high heterogeneity of systemic lupus erythematosus (SLE) pose great challenges to its diagnosis and treatment. The purpose of the current study is to identify genes and pathways involved in the pathogenesis of SLE. High throughput sequencing was performed on the PBMCs from SLE patients. We conducted differential gene analysis, gene ontology (GO) analysis, kyoto encyclopedia of genes and genomes (KEGG) analysis, and quantitative real-time PCR (qRT-PCR) verification. Protein–protein interaction (PPI) analysis, alternative splicing analysis, and disease correlation analysis were conducted on some key pathogenic genes as well. Furthermore, si-CDC6 was used for transfection and cell proliferation was monitored using a cell counting kit-8 (CCK-8) assay. We identified 2495 differential genes (1494 upregulated and 1001 downregulated) in SLE patients compared with healthy controls. The significantly upregulated genes were enriched in the biological process-related GO terms of the cell cycle, response to stress, and chromosome organization. KEGG enrichment analysis revealed 7 significantly upregulated pathways including SLE, alcoholism, viral carcinogenesis, cell cycle, proteasome, malaria, and transcriptional misregulation in cancer. We successfully verified some differential genes on the SLE pathway and the cell cycle pathway. CDC6, a key gene in the cell cycle pathway, had remarkably higher MXE alternative splicing events in SLE patients than that in controls, which may explain its significant upregulation in SLE patients. We found that CDC6 participates in the pathogenesis of many proliferation-related diseases and its levels are positively correlated with the severity of SLE. Knockdown of CDC6 suppressed the proliferation of Hela cells and PBMCs from SLE patients in vitro. We identified SLE-related genes and their alternative splicing events. The cell cycle pathway and the cell cycle-related biological processes are over-activated in SLE patients. We revealed a higher incidence of MXE events of CDC6, which may lead to its high expression in SLE patients. Upregulated cell cycle signaling and CDC6 may be related to the hyperproliferation and pathogenesis of SLE.
Design of a special rigid wheel for traversing loose soil
Authors: Elsheikh Mogeeb A. 
Published: January 2023

AbstractWheels play an important role in mobile robotics, wheelchairs and vehicles and represent an ideal solution for traversing rigid ground due to higher efficiency. Through traversing loose soil, the rigid wheels lose traction because of sinking and higher slip ratios. Therefore, the study suggests a new rigid wheel with a distinguished perimeter to increase mobility demands to overcome the previous inevitable concerns and clarifies its full detailed design. The lateral undulation locomotion of snakes inspired the author to introduce a new simple and affordable wheel design. The optimum values of the limbless creature movement on the sand are reflected in the geometrical parameters of the wheel, amplitude to wavelength ratio. In addition, the experimental work assessed the traveling performance of the fabricated wheel on the rigid ground and the sandy soil. The attained net traction and slip ratios approach the values of more complicated, expensive and heavier wheels that were used in farming and planetary exploration. Consequently, the wheel enables the wheeled locomotive to do missions on sandy soil with no trouble.
Dysfunctional epigenetic protein-coding gene-related signature is associated with the prognosis of pancreatic cancer based on histone modification and transcriptome analysis
Authors: Yu Xiao , Wang Yun , Shi Xiaoyi , Wang Zhihui , Wen Peihao , He Yuting , Guo Wenzhi 
Published: January 2023

AbstractEmerging evidence suggests that epigenetic alterations are responsible for the oncogenesis and progression of cancer. However, the role of epigenetic reprogramming in pancreatic cancer is still not clear. In this study, we used the limma R package to identify differentially expressed protein-coding genes (PCGs) between pancreatic cancer tissues and normal control tissues. The cell-type identification by the estimating relative subsets of RNA transcripts (CIBERSORT) package was used to quantify relative cell fractions in tumors. Prognostic molecular clusters were constructed using ConsensusClusterPlus analysis. Furthermore, the least absolute shrinkage and selection operator and stepAIC methods were used to construct a risk model. We identified 2351 differentially expressed PCGs between pancreatic cancer and normal control tissues in The cancer genome atlas dataset. Combined with histone modification data, we identified 363 epigenetic PCGs (epi-PCGs) and 19,010 non-epi-PCGs. Based on the epi-PCGs, we constructed three molecular clusters characterized by different expression levels of chemokines and immune checkpoint genes and distinct abundances of various immune cells. Furthermore, we generated a 9-gene model based on dysfunctional epi-PCGs. Additionally, we found that patients with high risk scores showed poorer prognoses than patients with low risk scores (p < 0.0001). Further analysis showed that the risk score was significantly related to survival and was an independent risk factor for pancreatic cancer patients. In conclusion, we constructed a 9-gene prognostic risk model based on epi-PCGs that might serve as an effective classifier to predict overall survival and the response to immunotherapy in pancreatic cancer patients.
Early influence of endotamponade on corneal biomechanical parameters, central corneal thickness and accuracy of intraocular pressure measurement
Authors: Pniakowska Zofia , Jurowski Piotr 
Published: January 2023

AbstractTo define the influence of air, SF6 gas and silicon oil 1000cs tamponade injection and oil tamponade removal on corneal biomechanics, central corneal thickness and intraocular pressure. 77 eyes referred to vitrectomy were divided into 4 groups: 19 to air tamponade, 21 to SF6 tamponade, 19 to oil tamponade, 18 to oil tamponade removal. Pre- and postoperative corneal hysteresis, corneal resistance factor, corneal thickness, Goldman intraocular pressure (GAT) and corneal compensated intraocular pressure (IOPcc) were analysed. GAT and IOPcc did not change after the air or SF6 tamponade. The oil tamponade injection caused increase in GAT and IOPcc, while the oil removal caused reduction in those parameters. In all groups, preoperative and postoperative values of GAT and IOPcc did not differ. There was no change in corneal thickness and biomechanics after air, SF6 or oil tamponade while after removal of oil those parameters are decreased. The air, SF6 and oil tamponade does not change the corneal thickness and corneal biomechanics. The removal of oil causes decrease in corneal thickness and biomechanics which can lead to bias in intraocular pressure measurement. GAT and IOPcc did not differ in eyes pre- and post-vitrectomy, being similarly reliable measure.
Toward a mechanistic understanding of adsorption behavior of phenol onto a novel activated carbon composite
Authors: Allahkarami Esmaeil , Dehghan Monfared Abolfazl , Silva Luis Felipe O. , Dotto Guilherme Luiz 
Published: January 2023

AbstractIn this research, the solid–liquid adsorption systems for MSAC (PbFe2O4 spinel-activated carbon)-phenol and pristine activated carbon-phenol were scrutinized from the thermodynamics and statistical physics (sta-phy) viewpoints. Experimental results indicated that MSAC composite outperformed pristine AC for the uptake of phenol from waste streams. By increasing the process temperature, the amount of phenol adsorbed onto both adsorbents, MSAC composite and pristine AC, decreased. Thermodynamic evaluations for MSAC demonstrated the spontaneous and exothermic characteristics of the adsorption process, while positive values of ΔG for pristine AC indicated a non-spontaneous process of phenol adsorption in all temperatures. In a mechanistic investigation, statistical physics modeling was applied to explore the responsible mechanism for phenol adsorption onto the MSAC composite and pristine AC. The single-layer model with one energy was the best model to describe the experimental data for both adsorbents. The adsorption energies of phenol onto both adsorbents were relatively smaller than 20 kJ/mol, indicating physical interactions. By increasing temperature from 298 to 358 K, the value of the absorbed amount of phenol onto the MSAC composite and pristine AC at saturation (Qsat) decreased from 158.94 and 138.91 to 115.23 and 112.34 mg/g, respectively. Mechanistic studies confirm the significant role of metallic hydroxides in MSAC to facilitate the removal of phenol through a strong interaction with phenol molecules, as compared with pristine activated carbon.
Profilin 1 deficiency drives mitotic defects and reduces genome stability
Authors: Scotto di Carlo Federica , Russo Sharon , Muyas Francesc , Mangini Maria , Garribba Lorenza , Pazzaglia Laura , Genesio Rita , Biamonte Flavia , De Luca Anna Chiara , Santaguida Stefano , Scotlandi Katia , Cortés-Ciriano Isidro , Gianfrancesco Fernando 
Published: January 2023

AbstractProfilin 1—encoded by PFN1—is a small actin-binding protein with a tumour suppressive role in various adenocarcinomas and pagetic osteosarcomas. However, its contribution to tumour development is not fully understood. Using fix and live cell imaging, we report that Profilin 1 inactivation results in multiple mitotic defects, manifested prominently by anaphase bridges, multipolar spindles, misaligned and lagging chromosomes, and cytokinesis failures. Accordingly, next-generation sequencing technologies highlighted that Profilin 1 knock-out cells display extensive copy-number alterations, which are associated with complex genome rearrangements and chromothripsis events in primary pagetic osteosarcomas with Profilin 1 inactivation. Mechanistically, we show that Profilin 1 is recruited to the spindle midzone at anaphase, and its deficiency reduces the supply of actin filaments to the cleavage furrow during cytokinesis. The mitotic defects are also observed in mouse embryonic fibroblasts and mesenchymal cells deriving from a newly generated knock-in mouse model harbouring a Pfn1 loss-of-function mutation. Furthermore, nuclear atypia is also detected in histological sections of mutant femurs. Thus, our results indicate that Profilin 1 has a role in regulating cell division, and its inactivation triggers mitotic defects, one of the major mechanisms through which tumour cells acquire chromosomal instability.
Older magma at Aso caldera than at Unzen stratovolcano in south west Japan as recorded through helium isotopes
Authors: Sano Yuji , Kagoshima Takanori , Zhang Maoliang , Takahata Naoto , Onoue Tetsuji , Shibata Tomo , Nishio Yoshiro , Chen Ai-Ti , Lee Hyunwoo , Fischer Tobias P. , Zhao Dapeng 
Published: January 2023

AbstractStratovolcanoes form conical mountains by ejecta and lava, while caldera volcanoes have depressions caused by post caldera collapse. Few studies have investigated relationships between this morphology difference and discharged fluid geochemistry. Here we present helium isotope ratios of geothermal fluids and groundwater inside and outside the caldera of Mt. Aso, Japan, and compare these to helium ratios in groundwater at Mt. Unzen, a stratovolcano in the same tectonic field. We find that helium isotopes decrease in proportion to distance from the central part of the volcano. However, the helium isotopes of inferred magmas are lower in Mt. Aso relative to Mt. Unzen. We combine this result with seismic tomography data and suggest that Mt. Aso may not be receiving new mantle material to the magma reservoir. Thus, we conclude that the caldera volcano Mt Aso probably features older magma and more developed geothermal systems than the stratovolcano Mt. Unzen.
Quaternary rainfall variability is governed by insolation in northern China and ice-sheet forcing in the South
Authors: Zhao Debo , Lu Zhengyao , Wan Shiming , Cheng Hai , Shi Xuefa , Li Anchun 
Published: January 2023

AbstractQuaternary Asian low-latitude hydroclimate cyclicity has long been attributed to insolation forcing, in contrast to the dominant ice-sheet and CO2 controls identified in mid-high-latitude regions. However, debates exist regarding these rainfall variations and forcings due to inconsistent reconstructions and simulations. Here, by combining rainfall proxy records with multi-model simulations, dominant 23 ka rainfall cycle in northern China and 100 ka rainfall cycle in southern China and Southeast Asia were found. We propose that rainfall mainly occurs in summer in the north, primarily driven by insolation. Rainfall in the south is largely forced by high-latitude ice sheets, with enhanced spring and autumn rainfall in southern China and weakened rainfall in western Maritime Continent during glacial periods. This study highlights the seasonal contributions to orbital-scale rainfall changes, and sheds light on the Asian hydroclimate conditions associated with high-low-latitude climate interactions.
A comprehensive characterization of cell-free RNA in spent blastocyst medium and quality prediction for blastocyst
Authors: Shi Huajuan , Pan Min , Jia Erteng , Lu Wenxiang , Zhou Ying , Sheng Yuqi , Zhao Xiangwei , Cai Lingbo , Ge Qinyu 
Published: January 2023

Abstract
The rate of pregnancy can be affected by many factors in assisted reproductive technology (ART), and one of which is the quality of embryos. Therefore, selecting the embryos with high potential is crucial for the outcome. Fifteen spent blastocyst medium (SBM) samples were collected from 14 patients who received in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), seven from high-grade embryos and eight from low-grade embryos. Cell-free RNA (cf-RNA) profile of SBM samples were analyzed by RNA sequencing in the present study. It was found that a large amount of cf-RNA were released into SBM, including protein-coding genes (68.9%) and long noncoding RNAs (lncRNAs) (17.26%). Furthermore, a high correlation was observed between blastocyst genes and SBM genes. And the cf-mRNAs of SBM were highly fragmented, and coding sequence (CDS) and untranslated (UTR) regions were released equally. Two hundred and thirty-two differentially expressed genes were identified in high-grade SBM (hSBM) and low-grade SBM (lSBM), which could be potential biomarker in distinguishing the embryos with different quality as an alternative or supplementary approach for subjective morphology criteria. Hence, cf-RNAs sequencing revealed the characterization of circulating transcriptomes of embryos with different quality. Based on the results, the genes related to blastocyst quality were screened, including the genes closely related to translation, immune-signaling pathway, and amino acid metabolism. Overall, the present study showed the types of SBM cf-RNAs, and the integrated analysis of cf-RNAs profiling with morphology grading displayed its potential in predicting blastocyst quality. The present study provided valuable scientific basis for noninvasive embryo selection in ART by RNA-profiling analysis.