bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cyfip2 allelic variation in C57BL/6J and C57BL/6NJ mice alters free‐choice ethanol drinking but not binge‐like drinking or wheel‐running activity
Authors: Hartmann Matthew C. , McCulley Walter D. , Holbrook Sarah E. , Haney Megan M. , Smith Caitlin G. , Kumar Vivek , Rosenwasser Alan M. 
Published: June 2023

AbstractBackgroundSince the origin of the C57BL/6 (B6) mouse strain, several phenotypically and genetically distinct B6 substrains have emerged. For example, C57BL/6J mice (B6J) display greater voluntary ethanol consumption and locomotor response to psychostimulants and differences in nucleus accumbens synaptic physiology relative to C57BL/6N (B6N) mice. A non‐synonymous serine to phenylalanine point mutation (S968F) in the cytoplasmic FMR1‐interacting protein 2 (Cyfip2) gene underlies both the differential locomotor response to cocaine and the accumbal physiology exhibited by these substrains. We examined whether Cyfip2 allelic variation underlies B6 substrain differences in other reward‐related phenotypes, such as ethanol intake and wheel‐running activity.MethodsWe compared voluntary ethanol consumption, wheel‐running, and binge‐like ethanol drinking in male and female B6J and B6NJ mice. When substrain differences were observed, additional experiments were performed in two novel mouse models in which the B6N Cyfip2 mutation was either introduced (S968F) into the B6J background or corrected (F968S) via CRISPR/Cas9 technology.ResultsB6J consumed significantly more ethanol than B6NJ and allelic variation in Cyfip2 contributed substantially to this substrain difference. In contrast, B6NJ displayed significantly more daily wheel‐running than B6J, with Cyfip2 allelic variation playing only a minor role in this substrain difference. Lastly, no substrain differences were observed in binge‐like ethanol drinking.ConclusionsThese results contribute to the characterization of behavior‐genetic differences between B6 substrains, support previous work indicating that free‐choice and binge‐like ethanol drinking are dependent on partially distinct genetic networks, and identify a novel phenotypic difference between B6 substrains in wheel‐running activity.
Pathology explains various mechanisms of auto‐immune inflammatory peripheral neuropathies
Authors: Vallat Jean‐Michel , Mathis Stéphane 
Published: June 2023

AbstractAutoimmune neuropathies are a heterogeneous group of rare and disabling diseases in which the immune system is thought to target antigens in the peripheral nervous system: they usually respond to immune therapies. Guillain–Barré syndrome is divided into several subtypes including “acute inflammatory demyelinating polyradiculoneuropathy,” “acute motor axonal neuropathy,” “acute motor sensory neuropathy,” and other variants. Chronic forms such as chronic inflammatory demyelinating polyneuropathy (CIDP) and other subtypes and polyneuropathy associated with IgM monoclonal gammopathy; autoimmune nodopathies also belong to this group of auto‐immune neuropathies. It has been shown that immunoglobulin G from the serum of about 30% of CIDP patients immunolabels nodes of Ranvier or paranodes of myelinated axons. Whatever the cause of myelin damage of the peripheral nervous system, the initial attack on myelin by a dysimmune process may begin either at the internodal area or in the paranodal and nodal regions. The term “nodoparanodopathy” was first applied to some “axonal Guillain–Barré syndrome” subtypes, then extended to cases classified as CIDP bearing IgG4 antibodies against paranodal axoglial proteins. In these cases, paranodal dissection develops in the absence of macrophage‐induced demyelination. In contrast, the mechanisms of demyelination of other dysimmune neuropathies induced by macrophages are unexplained, as no antibodies have been identified in such cases. The main objective of this presentation is to show that the pathology illustrates, confirms, and may explain such mechanisms.
Children can represent complex social status hierarchies: Evidence from Indonesia
Authors: Amemiya Jamie , Widjanarko Kiara , Chung Irene , Bian Lin , Heyman Gail D. 
Published: June 2023

AbstractChildren's ethnicity‐status associations are often studied in societies where one ethnic group possesses status across multiple dimensions, such as political influence and wealth. This study examined children's (6–12 years) and adults' representations of more complex hierarchies in Indonesia (N = 341; 38% Native Indonesian, 33% Chinese Indonesian, and 27% other ethnicities; 55% female, 36% male; 2021–2022), a society where ethnic groups hold distinct forms of status (on average, Native Indonesians have political influence; Chinese Indonesians have wealth). By 6.5 years, children associated Native Indonesians with political influence and Chinese Indonesians with wealth. Intersectional analyses indicated that ethnicity‐status associations were stronger for male than female targets. Children of all ethnicities preferred Chinese Indonesians and preferences were predicted by wealth judgments.
A special section: Recruiting and retaining couples from underrepresented backgrounds in intervention research
Authors: Mitchell Erica A. , Gordon Kristina Coop 
Published: June 2023

AbstractThis special section represents a collection of papers on recruitment and retention of couples from underrepresented backgrounds in couple intervention research. Research shows that couples from underrepresented backgrounds tend to be missing from intervention research. This gap is concerning; conclusions about the effectiveness of these interventions are not being drawn from diverse and representative samples and it may be that scholars are inadvertently creating inappropriate and inaccessible services for these couples. Recruiting and retaining these couples require specialized efforts and attention. In this summary paper, we describe (a) the origins of this special section, (b) the existing research on recruitment and retention in couple intervention research, (c) an overview of the papers in this special section, and (d) future recommendations and directions for this aspect of methodology in couple research. This collection of papers elevates the need to involve community members from the beginning, reduce barriers to access, and create recruitment materials and a service delivery environment that is specific for the target population.
Carbodiimide‐mediated grafting of caffeic acid on chitosan to improve its physicochemical and biological properties: used for Pompano (Trachinotus ovatus) preservation
Authors: Lan Weiqing , Zhang Bingjie , Liu Shucheng , Sun Yuqing , Xie Jing 
Published: June 2023

SummaryChitosan‐grafted caffeic acid (CS‐g‐CA) was successfully prepared using carbodiimide‐based chemical coupling method. The results of UV–vis and FT‐MIR showed that caffeic acid (CA) was successfully grafted onto chitosan (CS). The grafting degree of CA in CS determined by Folin–Ciocalteu scheme was up to 91.26 ± 2.58 mg g−1. Compared with CS solution, CS‐g‐CA solution has lower apparent viscosity and thermal stability. The results of SEM demonstrated that the grafting treatment changed the microstructure of CS. The grafting of the CA on CS enhanced its antioxidant activity. It can be seen that the clearance rate of DPPH (82.52% ± 0.7%), ABTS+ free radicals (88.21% ± 0.26%), T‐AOC (94.91% ± 0.21%) and FRAP (13.51 ± 0.52 mmol L−1 FeSO4) of CS‐g‐CA was similar to ascorbic acid at 2.0 mg mL−1. The results of antibacterial experiments demonstrated that CS‐g‐CA had better antibacterial ability in vitro, and the diameter of inhibition zone of 20.0 mg mL−1 CS‐g‐CA against Pseudomonas fluorescens and Staphylococcus saprophyticus was 16.88 ± 0.05 mm and 19.17 ± 0.09 mm, respectively, and it was significantly higher than CS (11.25 ± 0.04 mm and 13.09 ± 0.07 mm). The texture and freshness of Control group and CS rapidly declines post‐mortem. CS‐g‐CA treatment could effectively retard the deterioration of texture and keep the better quality of Pompanos (Trachinotus ovatus). This study indicates that grafting CA onto CS alters the biological activity and physicochemical properties of CS.
Fermentative extraction of phenolic compounds from unripe areca nuts by solid‐state fermentation using newly isolated Rhizopus orizaeMW538932
Authors: Hugar Priyanka , Belur Prasanna D. , Raval Keyur 
Published: June 2023

SummaryAreca nut (pericarp of Areca catechu L.) is a rich source of valuable phenolic compounds. Presence of a psychoactive agent, Arecoline, pose a challenge in the use of areca nut extracts. With an aim to maximise the extraction of phenolic compounds, a newly isolated Rhizopus orizae MW538932, was employed for the solid‐state fermentation of unripe areca nut (6–7 months' maturity) powder. Supplementary nutrients (carbon and nitrogen sources) for the media and the solvents for the extraction of phenolic compounds from the fermented medium were optimised. The optimised process could produce an extract having a total phenolic content of 186.03 ± 2.50 mg gallic acid equivalent and total flavonoid content of 139.70 ± 2.00 mg catechin equivalent per gram of the sample. UHPLC–MS/MS studies and HPLC analysis showed the presence of plethora of phenolic compounds and the absence of Arecoline and other alkaloids. This flavonoid‐rich extract can be a potential source of natural antioxidants for food and pharmaceutical industry.
A divided community: A descriptive qualitative study of the impact of the COVID‐19 pandemic on nurses and their relationships
Authors: Morley Georgina , Copley Dianna Jo , Field Rosemary B. , Zelinsky Megan , Albert Nancy M. 
Published: June 2023

AbstractAimsTo identify the personal and professional impact of the COVID‐19 pandemic on clinical nurses with regard to personal and workplace safety, personal and professional relationships and perceptions of their team, organization and community, and to understand lessons learned to inform future responses to pandemics or global emergencies.DesignQualitative, descriptive free‐text surveys, informed by appreciative inquiry.MethodsNurses working in adult COVID‐ and non‐COVID cohort medical‐surgical and intensive care units, outpatient cancer and general surgery centres were invited to participate. Data were collected between April and October 2021 and analysed using summative content analysis.ResultsIn total, 77 participants completed free‐text surveys. Five themes were identified: (1) Constraints on nursing: barriers in communication and diminished patient safety and quality of care; (2) Navigating uncertainty: the emotional toll of the pandemic; (3) Team solidarity, renewed appreciation and reaffirming purpose in nursing work; (4) Enhanced trust versus feeling expendable; and (5) Increased isolation and polarization within communities. Nurses described a perceived negative impact on a number of their relationships, including with patients, employer and community. They described a huge emotional toll that included feelings of isolation and polarization. While some nurses described feeling supported by their team and employer, others described feeling expendable.ConclusionNurses’ responses provided insights into negative emotional experiences during the pandemic due to heightened uncertainty and fear, and also the importance of support received from peers, colleagues and their employer. Nurses experienced feelings of isolation and polarization within their communities. The varied responses reflect the importance of societal solidarity when faced with global emergencies, and the need for nurses to feel valued by their patients and employer.ImpactEffective responses to public health emergencies require individuals and communities to work together to achieve collective goals. Efforts to retain nurses are critical during global emergencies.Patient or Public ContributionNo patient and public involvement.
Nurse–patient sexual health communication in gynaecological cancer follow‐up: A qualitative study from nurses' perspectives
Authors: Paulsen Anita , Vistad Ingvild , Fegran Liv 
Published: June 2023

AbstractAimTo explore nurse–patient sexual health communication from the perspectives of nurses trained to address sexual health in gynaecological cancer follow‐up.DesignA qualitative hermeneutic approach.MethodsIndividual semi‐structured interviews with 10 nurses at five different hospitals in Norway were conducted in March and April 2021. A Gadamerian‐inspired research method was used in the analysis.ResultsThree main themes with six sub‐themes were identified. The three main themes were: (1) building relationships through communication, (2) practice makes perfect—the importance of experience and knowledge, and (3) personal attitudes as promoters or inhibiters of sexual health communication.ConclusionThis study provides valuable insights into nurse–patient sexual health communication from the perspectives of nurses. The nurses in this study experienced the importance of having a good, respectful nurse–patient relationship as the foundation for sexual health communication. The professional confidence gained through experience and knowledge was emphasized, including the significance of how attitudes and taboos can influence sexual health communication.ImpactThe main findings of this study indicate that training in sexual health communication and the possibility of addressing sexual health repeatedly give nurses skills and professional confidence to address sexual health in cancer follow‐up. Our study indicates that sexual health communication can be achieved in a clinical setting without being overly resource demanding. Our results may also motivate nurses to enhance their knowledge about sexual health in cancer follow‐ups.Patient or Public ContributionA patient representative from the Norwegian Gynaecological Cancer Society have been involved in the planning of this study. She has given valuable contributions from the view of a gynaecological cancer patient.
Long‐term outdoor recreation program for adults with intellectual disabilities: Feasibility and effects
Authors: Barak Sharon , Dunsky Ayelet 
Published: June 2023

AbstractBackgroundWhile people with intellectual disabilities tend to refrain from physical activity, outdoor physical activity programs increase motivation to engage in physical activity.MethodEighty adults with intellectual disabilities participated in a 12‐month outdoor physical activity program. Attendance was used to assess feasibility. Aerobic capacity (6‐min walk test), lower extremity endurance (30‐s chair stand), and mobility (timed up and go) were assessed at three‐time points: before, during, and after the program. Six interviews were also conducted with six staff members and participants.ResultsThe physical activity program was feasible, with all six groups completing the year‐long activity. The six‐minute walk and timed‐up‐and‐go tests improved significantly. The qualitative analysis indicates the program's strengths (instructors' qualities and programs' social component) and weaknesses (dependency on weather and bureaucracy).ConclusionAmong adults with intellectual disabilities, a long‐term outdoor physical activity program is feasible as a means for increasing aerobic capacity and improving mobility ability.
Incidence and characteristics of ovarian cancer following endometrial cancer in the emerging era of conservative management of endometrial cancer—Implications for counseling—A SEER analysis
Authors: Nahshon Chen , Segev Yakir , Schmidt Meirav , Ostrovsky Ludmila , Assaf Wissam , Gerszman Eden , Lavie Ofer 
Published: June 2023

AbstractObjectiveTo assess the ovarian cancer (OC) risk following endometrial cancer (EC) in patients who underwent ovarian preservation as part of the EC staging.Study DesignWith permission of the Surveillance, Epidemiology and End Results (SEER) program of the United States National Cancer Institute, clinicopathological information of women diagnosed with EC and following OC were analyzed. Incidence of OC and survival according to the surgical approach were studied. Primary analysis was conducted in women up to and including the age of 49 years.ResultsA total of 116 patients up to the age of 49 years were diagnosed with EC and following OC. In this group of patients, no differences in incidence (IRR 0.9, CI 0.56–1.49, p = 0.66) or survival rates (p = 0.71) were found comparing ovarian preservation and bilateral salpingo‐oophorectomy (BSO) performance. In an overall analysis of women diagnosed with EC and following OC at any age, incidence of OC did not differ between groups (IRR 1.07, CI 0.83–1.39, p = 0.59) yet when including patients older than 49 years old survival rates were shorter in ovarian preservation patients compared to patients with BSO performed as part of their EC treatment.ConclusionOvarian preservation in EC patients under the age of 49 years may be considered safe, with no impact on OC incidence or survival, benefiting longer natural hormonal status.
The triglyceride catabolism regulated by a serine/threonine protein phosphatase, Smek1, is required for development and plant infection in Magnaporthe oryzae
Authors: Huang Zhicheng , Cao Huijuan , Wang Huan , Huang Pengyun , Wang Jing , Cai Ying‐Ying , Wang Qing , Li Yan , Wang Jiaoyu , Liu Xiao‐Hong , Lin Fu‐Cheng , Lu Jianping 
Published: June 2023

AbstractMagnaporthe oryzae is a pathogenic fungus that seriously harms rice production. Phosphatases and carbon metabolism play crucial roles in the growth and development of eukaryotes. However, it remains unclear how serine/threonine phosphatases regulate the catabolism of triglycerides, a major form of stored lipids. In this study, we identified a serine/threonine protein phosphatase regulatory subunit, Smek1, which is required for the growth, conidiation, and virulence of M. oryzae. Deletion of SMEK1 led to defects in the utilization of lipids, arabinose, glycerol, and ethanol. In glucose medium, the expression of genes involved in lipolysis, long‐chain fatty acid degradation, β‐oxidation, and the glyoxylate cycle increased in the Δsmek1 mutant, which is consistent with ΔcreA in which a carbon catabolite repressor CREA was deleted. In lipid medium, the expression of genes involved in long‐chain fatty acid degradation, β‐oxidation, the glyoxylate cycle, and utilization of arabinose, ethanol, or glycerol decreased in the Δsmek1 mutant, which is consistent with Δcrf1 in which a transcription activator CRF1 required for carbon metabolism was deleted. Lipase activity, however, increased in the Δsmek1 mutant in both glucose and lipid media. Moreover, Smek1 directly interacted with CreA and Crf1, and dephosphorylated CreA and Crf1 in vivo. The phosphatase Smek1 is therefore a dual‐function regulator of the lipid and carbohydrate metabolism, and controls fungal development and virulence by coordinating the functions of CreA and Crf1 in carbon catabolite repression (CCR) and derepression (CCDR).
Fibroblast activating protein promotes the proliferation, migration, and activation of fibroblasts in oral submucous fibrosis
Authors: Li Ming , Deng Zhiyuan , Xie Changqin , Chen Juan , Yuan Zhenying , Rahhal Omar , Tang Zhangui 
Published: June 2023

AbstractObjectivesFibroblast activating protein (FAP) is associated with various organ fibrosis. However, the expression and molecular function of FAP in oral submucous fibrosis (OSF) is still unclear.Materials and MethodsThe high‐performance liquid chromatography was used to detect the presence of alkaloids in areca nut extract (ANE). Real‐time qPCR, Western blot, and Immunohistochemistry assay were used to analyze the expression of FAP mRNA or protein in OSF and normal oral tissue. A chi‐squared test analyzed the relationship between FAP protein expression and clinicopathological data of OSF patients. CCK‐8, Wound‐healing, and Transwell migration assay were employed to assess the effect of the proliferation and migration ability of hOMF cells with FAP overexpression or knockdown. The expression level of a‐SMA, FSP1, and P13K–Akt signaling pathways‐related protein in hOMF cells transfected with FAP overexpression or knockdown plasmid was verified by western blot assay.ResultsThe four specific areca alkaloids (Arecoline, Guvacine, Arecaidine, and Guvacoline) were successfully detected in the ANE. The viability of hOMF cells was significantly improved in the 50 μg/mL ANE group and was inhibited in the 5 and 50 mg/mL ANE groups. The expression of FAP was upregulated in OSF tissues, and hOMF cells treated with 50 μg/mL ANE and was related to pathology grade, clinical stage, and history of chewing betel nut. Additionally, FAP may promote the proliferation, migration, and activation of hOMF cells through the P13K–Akt signaling pathway.ConclusionsThis study found that ANE had a bidirectional effect on the viability of hOMF cells, and the FAP gene was a potential therapeutic target in OSF.
Experiences of knowledge translation among researchers in transfusion medicine: Findings from an international survey study
Authors: Thijsen Amanda , Williamson Anna , Davison Tanya E. , Masser Barbara 
Published: June 2023

AbstractBackgroundTranslation of research knowledge is critical to ensure transfusion medicine policies and practices reflect current evidence and so effectively support the health of blood donors and recipients, as well as ensuring ongoing blood supply. The aim of this study was to investigate the barriers and facilitators of knowledge translation (KT) among transfusion medicine researchers and determine what KT supports are needed.Study Design and MethodsAn anonymous, cross‐sectional survey was distributed by emailing corresponding authors of papers in four major blood journals, emailing grant recipients in the area of transfusion medicine, posting on social media, and through an international blood operator network.ResultsThe final sample included 105 researchers. Participants had a positive orientation toward KT, with few perceiving KT as not relevant to their research or beneficial for their careers. However, many reported facing difficulties practicing KT due to time constraints, competing priorities, or lack of funds or resources. Fostering relationships with stakeholders was seen as a key facilitator of KT but a number of researchers expressed difficulties engaging and communicating with them. Collaboration opportunities, protected time for KT, and access to KT resources were some of the supports researchers felt were required to help their KT efforts.ConclusionTo minimize the knowledge to practice gap in transfusion medicine and ensure findings from research lead to improved outcomes, organizations need to support researchers in their KT efforts and facilitate interactions between researchers and research end‐users.