bluuebunny/crossref_metadata_2025_split huggingface dataset:
A method of underwater sound source range estimation without prior knowledge based on single sensor in shallow water
Authors: Li Xiaoman , Chen Hongyun , Lu Hongyu , Bi Xuejie , Mo Yaxiao 
Published: March 2023

Introduction: The lack of prior knowledge of the marine environment increases the difficulty of passive ranging of underwater sound sources by using a single hydrophone. The dispersion curve of the normal mode contains extensive marine environmental information, which can be extracted without prior knowledge, but the characteristics of dispersion curves of different modes vary, and the mode order cannot be determined from the received data.Methods: Herein, a method based on a single hydrophone that can jointly identify the mode order and estimate the propagation range in unknown marine environment is proposed. The method uses Bayesian theory as the main methodology and is applicable to broadband pulse sound sources in shallow seas with long-range propagation. The dispersion curves extracted from the data and those calculated by the dispersion formula are the input signal and the replica of the methods, respectively. Accurate identification of the normal mode order and estimation of the propagation range can be achieved by establishing the joint cost function.Results: In the case of unknown a priori knowledge of the marine environment, the method enables rapid inversion, is tolerant to environmental parameter mismatch, and is low cost and practical.Discussion: The simulation and measured data analysis results demonstrate the accuracy and validity of the method. The measured data contains linear frequency modulation impulse source signal and explosion sound source signals, and the mean relative error of range estimation is less than 5%.
Effects of hypoxia on bronchial and alveolar epithelial cells linked to pathogenesis in chronic lung disorders
Authors: Berggren-Nylund Rebecca , Ryde Martin , Löfdahl Anna , Ibáñez-Fonseca Arturo , Kåredal Monica , Westergren-Thorsson Gunilla , Tufvesson Ellen , Larsson-Callerfelt Anna-Karin 
Published: March 2023

Introduction: Chronic lung disorders involve pathological alterations in the lung tissue with hypoxia as a consequence. Hypoxia may influence the release of inflammatory mediators and growth factors including vascular endothelial growth factor (VEGF) and prostaglandin (PG)E2. The aim of this work was to investigate how hypoxia affects human lung epithelial cells in combination with profibrotic stimuli and its correlation to pathogenesis.Methods: Human bronchial (BEAS-2B) and alveolar (hAELVi) epithelial cells were exposed to either hypoxia (1% O2) or normoxia (21% O2) during 24 h, with or without transforming growth factor (TGF)-β1. mRNA expression of genes and proteins related to disease pathology were analysed with qPCR, ELISA or immunocytochemistry. Alterations in cell viability and metabolic activity were determined.Results: In BEAS-2B and hAELVi, hypoxia significantly dowregulated genes related to fibrosis, mitochondrial stress, oxidative stress, apoptosis and inflammation whereas VEGF receptor 2 increased. Hypoxia increased the expression of Tenascin-C, whereas both hypoxia and TGF-β1 stimuli increased the release of VEGF, IL-6, IL-8 and MCP-1 in BEAS-2B. In hAELVi, hypoxia reduced the release of fibroblast growth factor, epidermal growth factor, PGE2, IL-6 and IL-8, whereas TGF-β1 stimulus significantly increased the release of PGE2 and IL-6. TGF-β1 stimulated BEAS-2B cells showed a decreased release of VEGF-A and IL-8, while TGF-β1 stimulated hAELVi cells showed a decreased release of PGE2 and IL-8 during hypoxia compared to normoxia. Metabolic activity was significantly increased by hypoxia in both epithelial cell types.Discussion: In conclusion, our data indicate that bronchial and alveolar epithelial cells respond differently to hypoxia and profibrotic stimuli. The bronchial epithelium appears more responsive to changes in oxygen levels and remodelling processes compared to the alveoli, suggesting that hypoxia may be a driver of pathogenesis in chronic lung disorders.
Metabolic correlates to critical speed in murine models of sickle cell disease
Authors: Cendali Francesca I. , Nemkov Travis , Lisk Christina , Lacroix Ian S. , Nouraie Seyed-Mehdi , Zhang Yingze , Gordeuk Victor R. , Buehler Paul W. , Irwin David , D’Alessandro Angelo 
Published: March 2023

Introduction: Exercise intolerance is a common clinical manifestation in patients with sickle cell disease (SCD), though the mechanisms are incompletely understood.Methods: Here we leverage a murine mouse model of sickle cell disease, the Berkeley mouse, to characterize response to exercise via determination of critical speed (CS), a functional measurement of mouse running speed upon exerting to exhaustion.Results: Upon observing a wide distribution in critical speed phenotypes, we systematically determined metabolic aberrations in plasma and organs—including heart, kidney, liver, lung, and spleen—from mice ranked based on critical speed performances (top vs. bottom 25%). Results indicated clear signatures of systemic and organ-specific alterations in carboxylic acids, sphingosine 1-phosphate and acylcarnitine metabolism. Metabolites in these pathways showed significant correlations with critical speed across all matrices. Findings from murine models were thus further validated in 433 sickle cell disease patients (SS genotype). Metabolomics analyses of plasma from 281 subjects in this cohort (with HbA &lt; 10% to decrease confounding effects of recent transfusion events) were used to identify metabolic correlates to sub-maximal exercise test performances, as measure by 6 min walking test in this clinical cohort. Results confirmed strong correlation between test performances and dysregulated levels of circulating carboxylic acids (especially succinate) and sphingosine 1-phosphate.Discussion: We identified novel circulating metabolic markers of exercise intolerance in mouse models of sickle cell disease and sickle cell patients.
From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization of Eragrostis curvula
Authors: Carballo Jose , Bellido Andrés Martin , Selva Juan Pablo , Zappacosta Diego , Gallo Cristian Andres , Albertini Emidio , Caccamo Mario , Echenique Viviana 
Published: March 2023

IntroductionIn Eragrostis curvula, commonly known as weeping lovegrass, a synthetic diploidization event of the facultative apomictic tetraploid Tanganyika INTA cv. originated from the sexual diploid Victoria cv. Apomixis is an asexual reproduction by seeds in which the progeny is genetically identical to the maternal plant.MethodsTo assess the genomic changes related to ploidy and to the reproductive mode occurring during diploidization, a mapping approach was followed to obtain the first E. curvula pangenome assembly. In this way, gDNA of Tanganyika INTA was extracted and sequenced in 2x250 Illumina pair-end reads and mapped against the Victoria genome assembly. The unmapped reads were used for variant calling, while the mapped reads were assembled using Masurca software.ResultsThe length of the assembly was 28,982,419 bp distributed in 18,032 contigs, and the variable genes annotated in these contigs rendered 3,952 gene models. Functional annotation of the genes showed that the reproductive pathway was differentially enriched. PCR amplification in gDNA and cDNA of Tanganyika INTA and Victoria was conducted to validate the presence/absence variation in five genes related to reproduction and ploidy. The polyploid nature of the Tanganyika INTA genome was also evaluated through the variant calling analysis showing the single nucleotide polymorphism (SNP) coverage and allele frequency distribution with a segmental allotetraploid pairing behavior.DiscussionThe results presented here suggest that the genes were lost in Tanganyika INTA during the diploidization process that was conducted to suppress the apomictic pathway, affecting severely the fertility of Victoria cv.
Postharvest light-induced flavonoids accumulation in mango (Mangifera indica L.) peel is associated with the up-regulation of flavonoids-related and light signal pathway genes
Authors: Zhu Wencan , Wu Hongxia , Yang Chengkun , Shi Bin , Zheng Bin , Ma Xiaowei , Zhou Kaibing , Qian Minjie 
Published: March 2023

IntroductionFlavonoids are important secondary metabolites in plants and light is a crucial environmental factor regulating flavonoids biosynthesis. However, effect of light on the different flavonoids compositions accumulation in mango and the relevant molecular mechanism still need to be clarified.MethodsIn this study, green-mature fruits of red mango cultivar ‘Zill’ were subjected to postharvest light treatment, and fruit peel color, total soluble solids content, total organic acid, and firmness of flesh were measured. The flavonoids metabolites profile, and the expression of flavonoids-related genes and light signal pathway genes were also analyzed.ResultsResults showed that light treatment promoted the red coloration of fruit peel and increased the total soluble solids content and firmness of flesh. The concentration of flavonols, proanthocyanidins and anthocyanins, and expression of key flavonoids biosynthetic genes including MiF3H, MiFLS, MiLAR, MiANS, MiUFGT1, and MiUFGT3 were significantly induced by light. The MYBs regulating flavonols and proanthocyanidins, i.e. MiMYB22 and MiMYB12, as well as the key light signal pathway transcription factors (TFs) MiHY5 and MiHYH, were identified in mango. The transcription of MiMYB1, MiMYB12, MiMYB22, MiHY5 and MiHYH was up-regulated by light.DiscussionOur results provide a postharvest technology to improve mango fruit appearance quality, and are helpful to reveal the molecular mechanism of light-induced flavonoids biosynthesis in mango.
Long-term cattle manure addition enhances soil-available phosphorus fractions in subtropical open-field rotated vegetable systems
Authors: Mao Yanting , Hu Wei , Li Yongmei , Li Yuan , Lei Baokun , Zheng Yi 
Published: March 2023

IntroductionEvaluation of the changes in phosphorus (P) fractions (various P forms) and their availability at different soil layers is critical for enhancing P resource use efficiency, mitigating subsequent environmental pollution, and establishing a suitable manure application strategy. However, changes in P fractions at different soil layers in response to cattle manure (M), as well as a combined cattle manure and chemical fertilizer application (M+F), remain unclear in open-field vegetable systems. If the amount of annual P input remains the same, identifying which treatment would cause a higher phosphate fertilizer use efficiency (PUE) and vegetable yield while simultaneously reducing the P surplus is especially warranted.MethodsBased on a long-term manure experiment that started in 2008, we used a modified P fractionation scheme to analyze P fractions at two soil layers for three treatments (M, M+F, and control without fertilizer application) in an open-field cabbage (Brassica oleracea) and lettuce (Lactuca sativa) system, and assessed the PUE and accumulated P surplus.ResultsThe concentrations of the soil P fractions were higher in the 0–20-cm soil layer compared to the 20–40-cm layer, except for organic P (Po) and residual-P. M application significantly increased the inorganic P (Pi) (by 8.92%–72.26%) and the Po content (by 5.01%–61.23%) at the two soil layers. Compared with the control and M+F treatments, M significantly increased residual-P, Resin-P, and NaHCO3-Pi at both soil layers (by 31.9%–32.95%, 68.40%–72.60%, and 48.22%–61.04%), whereas NaOH-Pi and HCl-Pi at 0–20 cm were positively correlated with available P. Soil moderately labile-P was the predominant P component in the two soil layers (accounting for 59%–70%). With the same annual P input amount, M+CF created the highest vegetable yield (117.86 t ha-1), and PUE (37.88%) and M created the highest accumulated P surplus (128.80 kg ha-1yr-1).DiscussionCollectively, a combined manure-chemical fertilizer application has great potential to yield a long-term positive outcome both in terms of vegetable productivity and environmental health in open-field vegetable systems. This highlights the methods’ benefits as a sustainable practice in subtropical vegetable systems. Specific attention should be given to a P balance to avoid excessive P input if a rational strategy for manure application is to be attained. This is especially the case for stem vegetables that require manure application and decreases the environmental risk of P loss in vegetable systems.
The associations between social environment and adolescents’ psychosomatic health: An ecological perspective
Authors: Huang Yi , Lu Jinjin , Širůček Jan 
Published: March 2023

ObjectivesIt has been known that social environments are associated with adolescents’ health. However, the complex relationship between diverse types of social environments and adolescents’ psychosomatic heath remained unclear. Thus, using an ecological perspective, the current study aimed to examine the associations between social environment and adolescents’ psychosomatic health.MethodsWe used the data from the Health Behavior in School-aged Children (HBSC) project conducted in the Czech Republic in 2018. A total of 13377 observations were included.ResultsThe region, as a macrosystem, could not explain the variance in adolescents’ psychological and somatic health. The quality of neighborhood environment (exosystem) was significantly related to adolescents’ psychological and somatic health. At the microsystem level, teacher support had stronger, family support had weaker, and peer support had no association with psychological and somatic health. At the mesosystem level, the interactions between family, teacher, and friend support were negligible for adolescents’ psychological and somatic health.ConclusionsThe results underscore the importance of teachers’ support and neighborhood environment for adolescents’ psychosomatic health. Therefore, the findings suggest the need to improve teacher-adolescent relationships and the neighborhood community quality.
The impact of the war in Ukraine on the perinatal period: Perinatal mental health for refugee women (pmh-rw) protocol
Authors: Rodríguez-Muñoz Maria F. , Chrzan-Dętkoś Magdalena , Uka Ana , García-López Helena S. , Krupelnytska Liudmyla , Morozova-Larina Olha , Vavilova Alona , Molotokas Antonina , Murawska Natalia , Le Huynh-Nhu 
Published: March 2023

BackgroundThe aim of the Perinatal Mental Health for Refugee Women (PMH-RW) Project is to investigate the impact of the war on perinatal mental health: anxiety, post-traumatic stress, depression, and birth trauma symptoms. It will also evaluate the factors that serve as protective elements for the development of these potential diagnoses (such as personality traits, social support, sociodemographic characteristics, and access to medical/mental health services).MethodAn international observational cohort study with baseline data is being assessed in Ukraine (for internal refugees) and several European Countries (for external refugees). The study includes as participants both pregnant women and those who have recently given birth (with babies up to 1 year old). The assessment includes measures on depression (EPDS), anxiety (GAD-7), experiences during birth (City Birth Questionnaire), post-traumatic stress symptoms [Impact of events scale—revised (PTSD-R)], Personality (10-Item Personality Inventory-TIPI), and a questionnaire for socio-demographic data which also such social support.ConclusionThis study will provide needed information for determining the impact of the Ukrainian Crisis on perinatal mental health by studying potential risk and protective factors. The data collected will be used to inform policymakers with useful information that can be used in the development of plans to protect and promote the mental health of the perinatal refugees impacted by this event. Also, it is our hope that data collected from this study will serve to plant the seeds for further research regarding the impact of the crisis in Ukraine on the offspring and to analyze how these events are affecting further generations.Clinical Trial RegistrationClinicalTrials.gov, Identifier: NCT05654987.
A comparative study of well-being, resilience, mindfulness, negative emotions, stress, and burnout among nurses after an online mind–body based intervention during the first COVID-19 pandemic crisis
Authors: Cepeda-Lopez Ana Carla , Solís Domínguez Leticia , Villarreal Zambrano Sofía , Garza-Rodriguez Iris Y. , del Valle Alejandra Cortes , Quiroga-Garza Angélica 
Published: March 2023

BackgroundTo mitigate against the possible adverse effects of stress among nurses due to the COVID-19 outbreak, we designed a 12-week mind–body based online intervention program to promote well-being and prevent stress-related disorders such as burnout. Our study aimed to compare the impact of the intervention on perception of stress, negative emotions, burnout, mindfulness, resilience, and well-being at pretest and 6 months post-intervention and to compare the effect among nurses working at two different hospitals.MethodsWe conducted an uncontrolled trial using a convenience sample of nurses working at two hospitals in Mexico: one designated to treat confirmed COVID-19 patients (COVID-hospital) and the other whose patients had a negative COVID-19 test on admission (Non COVID-hospital). The 12 week online intervention consisted of 36 mind–body based micropractices, with subjective well-being as the primary outcome. Secondary outcomes were health perception, resilience, mindfulness, negative emotions, stress, and burnout.ResultsA pretest survey was completed by 643 nurses. Of the remaining valid responses, 82% were women, with a mean age of 34.8 (SD = 8.95) years old. For the analysis two groups of nurses were sampled by cluster: a COVID-hospital group of 429 (67%) nurses, and a non-COVID Hospital group of 214 (33%) nurses. The proportion lost to follow-up was 71% at postest (n = 188) and 42% at 6 months follow-up (n = 371). At pretest, non-COVID hospital nurses had lower subjective well-being and higher burnout than their COVID hospital counterparts. At postest, non-COVID hospital nurses displayed more negative emotions than their COVID hospital peers. At 6 months post-intervention, nurses experienced improved mindfulness, reduced negative emotions and stress, but a decrease in subjective well-being and resilience. Nurses working at the non-COVID hospital had significantly higher mean scores for burnout than those working at the COVID hospital.ConclusionThe results of our study suggest that our online mind–body interventions can help to reduce stress and negative emotions, yet the effects on subjective well-being and resilience are uncertain. Further research is needed to gain a better understanding of their potential mechanisms and the associated efforts of such online interventions.Clinical Trial RegistrationClinicalTrials.gov, NCT05515172.
Smartwatch digital phenotypes predict positive and negative symptom variation in a longitudinal monitoring study of patients with psychotic disorders
Authors: Kalisperakis Emmanouil , Karantinos Thomas , Lazaridi Marina , Garyfalli Vasiliki , Filntisis Panagiotis P. , Zlatintsi Athanasia , Efthymiou Niki , Mantas Asimakis , Mantonakis Leonidas , Mougiakos Theodoros , Maglogiannis Ilias , Tsanakas Panayotis , Maragos Petros , Smyrnis Nikolaos 
Published: March 2023

IntroductionMonitoring biometric data using smartwatches (digital phenotypes) provides a novel approach for quantifying behavior in patients with psychiatric disorders. We tested whether such digital phenotypes predict changes in psychopathology of patients with psychotic disorders.MethodsWe continuously monitored digital phenotypes from 35 patients (20 with schizophrenia and 15 with bipolar spectrum disorders) using a commercial smartwatch for a period of up to 14 months. These included 5-min measures of total motor activity from an accelerometer (TMA), average Heart Rate (HRA) and heart rate variability (HRV) from a plethysmography-based sensor, walking activity (WA) measured as number of total steps per day and sleep/wake ratio (SWR). A self-reporting questionnaire (IPAQ) assessed weekly physical activity. After pooling phenotype data, their monthly mean and variance was correlated within each patient with psychopathology scores (PANSS) assessed monthly.ResultsOur results indicate that increased HRA during wakefulness and sleep correlated with increases in positive psychopathology. Besides, decreased HRV and increase in its monthly variance correlated with increases in negative psychopathology. Self-reported physical activity did not correlate with changes in psychopathology. These effects were independent from demographic and clinical variables as well as changes in antipsychotic medication dose.DiscussionOur findings suggest that distinct digital phenotypes derived passively from a smartwatch can predict variations in positive and negative dimensions of psychopathology of patients with psychotic disorders, over time, providing ground evidence for their potential clinical use.
The experiences of practitioners working with young people exposed to online sexual abuse
Authors: Quayle Ethel , Schwannauer Matthias , Varese Filippo , Cartwright Kim , Hewins William , Chan Cindy , Newton Alice , Chitsabesan Prathiba , Richards Cathy , Bucci Sandra 
Published: March 2023

IntroductionThis qualitative study explored healthcare professionals' current understanding of, and clinical practices related to, Online Child Sexual Abuse (OCSA).MethodsData were collected across two UK sites (Manchester and Edinburgh). Interviews and one focus group were held with 25 practitioners working in services offering clinical support to young people who have experienced OCSA. Thematic analysis of the data identified three overarching themes and 10 subthemes related to the research questions: (1) the breadth of the problem; (2) working with OCSA; and (3) the emotionally charged nature of OCSA.ResultsWhile practitioners recognized OCSA as problematic, they differed in how they conceptualized it. There was a heightened awareness of the role that sexual images played in OCSA and concerns about first-person-produced imagery by Children and Young People (CYP). Practitioners described a generational gap related to their technology use and that of the young people they worked with. Practitioners also described a paucity of referral pathways and concerns that there was no training available to them. Organizational barriers meant that questions about technology use were not routinely included in assessments and often there was reliance on young people making disclosures.DiscussionNovel findings from this study were the psychological impacts that such cases had on practitioners, which may indicate a need for organizational support for staff as well as further training needs. Existing frameworks that help conceptualize and assess the role of technology as part of the ecology of the child may have great utility for practitioners.