bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dexamethasone effects on the expression and content of glycosylated components of mouse brain tissue
Authors: Aladev S. D. , Sokolov D. K. , Strokotova A. V. , Kazanskaya G. M. , Volkov A. M. , Politko M. O. , Shahmuradova A. I. , Kliver E. E. , Tsidulko A. Y. , Aidagulova S. V. , Grigorieva E. V. 
Published: April 2023

Introduction. Glucocorticoids are actively used in the treatment of various diseases, however their long-term use leads to numerous negative side-effects, the molecular mechanisms of which remain poorly understood.Aim. Study of the short-term (1–10 days) effects of various doses of dexamethasone (Dex) (0,1–10 mg/kg) on the expression of the glucocorticoid receptor (GR, Nr3c1), core proteins of main proteoglycans and heparan sulfate metabolism-involved genes, as well as the content of carbohydrate macromolecules of glycosaminoglycans in the brain tissue of experimental animals.Materials and methods. In the study, C57Bl/6 mice were used. The expression of GR, proteoglycan core proteins and heparan sulfate metabolism-involved genes was determined by real-time polymerase chain reaction with reverse transcription. The content and localization of GR protein molecule were studied by Western blot and immunohistochemical analysis, and the glycosaminoglycan content was determined by dot-blot analysis and Alcian Blue staining.Results. It was shown that a single Dex administration leads to fast (1–3 days) short-term activation of GR expression (+1.5 times, p <0.05), proteoglycan’s genes (syndecan-3, Sdc3; perlecan, Hspg2; phosphacan, Ptprz1; neurocan, Ncan; +2–3-fold; p <0.05) and heparan sulfate-metabolism-involved genes (Ndst1, Glce, Hs2st1, Hs6st1, Sulf1 / 2; +1.5–2-fold; p <0.05) in the mouse brain, with a return to control values by 7–10 days after Dex administration. At the same time, the effect of Dex on carbohydrate macromolecules of glycosaminoglycans was more delayed and stable, increasing the content of low-sulfated glycosaminoglycans in the brain tissue in a dose-dependent manner starting from day 1 after Dex administration. Highly-sulfated glycosaminoglycans showed more delayed response to Dex administration, and an increase in their content was observed only at higher doses (2.5 and 10 mg/kg) and only on 7–10 days after its administration, apparently, mainly due to an increase in heparan sulfate content.Conclusion. In general, the effect of a single injection of Dex on the transcriptional activity of GR, proteoglycan core proteins and heparan sulfate metabolism-involved genes were short-termed, and the genes expression quickly returned to the normal levels. However, even a single use of Dex significantly increased the content of total as well as highly sulfated glycosaminoglycans in the mouse brain tissue, which can lead to the changes in the composition and structure of the brain tissue, as well as its functional characteristics.
Shallow Seismicity in the Long Beach–Seal Beach, California Area
Authors: Yang Yan , Clayton Robert W. 
Published: April 2023

AbstractSeismicity can help to locate fault zones that are often difficult to characterize in densely populated urban areas. In this study, we use three dense nodal arrays consisting of thousands of sensors to detect and locate seismic events in the Long Beach–Seal Beach area of California. Small events can be detected at sufficient signal-to-noise levels during the night, when urban noise is relatively low. We detect and locate &gt;1000 events with ML below 2. Most of the located events are clustered at very shallow depth (0–2 km). The results support previous suggestions that the shallow Newport-Inglewood fault is a wide splayed fault in this area. The seismicity pattern also compares well with some newly identified faults from reflection seismic surveys. The shallow events, which elude detection by the regional seismic network, underscore the complex nature of the faults and their seismic hazard.
Emotions in the Language of the Media
Authors: Białek-Szwed Olga , Wójciszyn-Wasil Aneta 
Published: April 2023

The objective of this article is to present major aspects of linguistic studies of emotions. The studies rely on cognitive linguistics methodology, which encompasses both the spoken and written language, as well as non-verbal communication. Communicative aspects are of great importance, making it possible to observe the interaction between language and emotions. Emotionally-charged lexis and so-called “hate speech” are exemplary manifestations of linguistic emotional expressions.
Contemporary studies of language used in the media concentrate on an analysis of means of linguistic expression, cognitive definitions and mechanisms which generate emotions. The results of this study can be applied to various types of the media, they can help the media reach their target audience and engage the audience in the presented message.
Emotion-Generating Mechanisms in Television Programmes
Authors: Sosnowska Joanna 
Published: April 2023

The aim of this article is to present the mechanisms of mediatisation of emotions in TV broadcasts in relation to the messages concerning the SARS-CoV-2 pandemic. The pandemic time has radically changed the way television works. The production of many TV programmes has been suspended around the world, and the way journalists and editorial staff work has changed, yet the viewership during the pandemic grew all over the world, despite negative emotions being generated.
The study relies on a qualitative analysis of the emotion-generating mechanisms in the audiovisual image. The research material was TV programmes about SARS-CoV-2 in March–June 2020. The research tools used permitted an analysis of the content of the message and its formal side (sound, image, graphics, music). The final results were divided into two areas of emotion-generating mechanisms: formal (forms) and thematic (content).
The analysis shows that especially negative emotions were evoked in TV programmes during the pandemic: threat, sadness and fear. At the level of form, the following mechanisms were revealed: visual enhancement, gradation, giving meaning, contrast and generalization. In terms of content mechanisms, one can observe the mechanism of drama, content accumulation (blocking), exposure, variable dynamics of positive and negative content, and narrative mechanisms. The observed mechanisms of generating emotions may also lead to manipulation of the recipient through the repetition of negative information or the use of authorities.
The conducted analysis confirmed the importance of combining form and content as well as strength, rhythmization and repetition of specific messages in the audiovisual message. The article may be the basis for research on the relationship between emotions and cognition, as well as research on the reception of audiovisual content.
Stage-by-Stage Hydraulic Fracture and Reservoir Characterization through Integration of Post-Fracture Pressure Decay Analysis and the Flowback Diagnostic Fracture Injection Test Method
Authors: Zeinabady D. , Clarkson C. R. 
Published: April 2023

Summary
The post-fracture pressure decay (PFPD) technique is a low-cost method allowing for stage-by-stage hydraulic fracture characterization. The analysis of the PFPD data is complex, with data affected by both hydraulic fracture and reservoir properties. Available analysis methods in the literature are oversimplified; reservoir or fracture properties are often assumed to be constant along the horizontal well, and therefore changes in the trend of pressure decay data are attributed to hydraulic fracture or reservoir properties only. Moreover, methods analogous to those applied to the analysis of conventional diagnostic fracture injection tests (DFITs) are often used and ignore critical mechanisms involved in main-stage hydraulic fracture stimulation.
A conceptual numerical simulation study was first conducted herein to understand the key mechanisms involved in main-stage hydraulic fracturing. An analytical model was then developed to account for the dynamic behavior of the hydraulic fracture, leakoff, proppant distribution, multiple fractures, and propped- and unpropped-closure events. The analytical model is cast in the form of a new straightline analysis (SLA) method that provides stage-by-stage estimates of the ratio of unpropped fracture surface area to total fracture surface area. The SLA method was validated against numerical simulation results. Moreover, to account for the variation of reservoir properties along the horizontal well, the PFPD model is integrated with DFIT-flowback (DFIT-FBA) tests, performed at some points along the lateral, to obtain a reliable stage-by-stage hydraulic fracture and reservoir characterization approach. The practical application of the proposed integrated approach was demonstrated using PFPD and DFIT-FBA data from a horizontal well completed in 22 stages in the Montney Formation.
The numerical simulation study demonstrated that the use of proppant and injection into multiple clusters (creating multiple fractures) results in multiple closure events. The closure process may start early after the pump-in period at a pressure significantly higher than the minimum in-situ stress. Using DFIT-based analytical models, which ignore the presence of proppant, causes significant errors in hydraulic fracture and reservoir property estimation. The PFPD field data examined herein exhibited a similar pressure trend to the numerical simulation cases. The ratio of unpropped fracture surface area to total fracture surface area was determined stage by stage using the PFPD SLA method, constrained by DFIT-FBA data. Engineers can use this information to optimize the hydraulic fracture stimulation design in real time, optimize the well spacing, and forecast the production. The cost and time advantages of this diagnostic method make this approach very attractive.
Apparent Heat of Reaction of Heavy Oil with Application of Accelerated Rate Calorimeter
Authors: Fazlyeva Rita , Mallory Donald , Moore Gordon , Modaresghazani Jafar , Mehta Sudarshan , Cheremisin Alexey 
Published: April 2023

Summary
A series of accelerated rate calorimeter (ARC) experiments were conducted on heavy oil mixed with a carbonate core to study the thermal behavior and oxygen uptake operating at different air fluxes of 5.0, 2.5, and 1.25 (m3(ST)/(m2h)) referred to as Runs -1, -2 and -3, respectively. As a result, evaluation of the temperature rise per unit mass of oxygen was proposed as a new approach for identifying the main contributing mechanism during in-situ combustion (ISC). The obtained findings can be used for predicting the transition occurring between low- and high-temperature ranges (LTR and HTR). For Runs -2 and -3, such a transition occurred at 280°C, whereas for Run-1, operated at the highest air flux, LTR shifted to HTR at about 300°C. The overlapping curves of O2 to COx and O2 to low-temperature oxidation (LTO) plus water were interpreted as equal contributors to energy formation. The calculated maximum values varied between 4053 for LTO reactions and 13 420 dT(°C)dmO2(g) for high-temperature oxidation (HTO) reactions depending on the air flux.
The measured self-heat rates (SHRs) and oxygen uptake, further used to obtain the apparent heat of reaction, were in general agreement with the values reported in the literature, 16 000 J/g O2 consumed. These values are typical for the HTO reactions; however, in this study, the heats of reaction when the oxidation reactions are operating in the oxygen addition or LTO mode were the main interest. Depending on the run, the apparent heats of LTO reactions varied between 4422 and 7101 J/g O2 consumed.
A Global Neuronopathic Gaucher Disease Registry (GARDIAN): A Patient-Led Initiative
Authors: Collin-Histed Tanya , Stoodley Madeline , Beusterien Kathleen , Elstein Deborah , Jaffe Dena H , Revel-Vilk Shoshana , Davies Elin Haf 
Published: April 2023

Abstract
Background
Gaucher disease (GD) is a rare autosomal recessive lysosomal storage disorder. GD types 2 and 3 are known as neuronopathic Gaucher disease (nGD) because they have brain involvement that progresses over time. Implementing a systematic approach to the collection of real-world clinical and patient-relevant outcomes data in nGD presents an opportunity to fill critical knowledge gaps and ultimately help healthcare providers in the management of this patient population. This paper summarizes the development of a patient-initiated Gaucher Registry for Development Innovation and Analysis of Neuronopathic Disease (GARDIAN).
Methods
The International Gaucher Alliance led the GARDIAN planning, including governance, scope, stakeholder involvement, platform, and reporting. Registry element input was determined in a series of meetings with clinical experts, patients, and caregivers, who identified key clinical variables and the draft content of nGD patient-reported outcomes (PRO) and observer-reported outcomes (ObsRO) focusing on symptoms, patient physical and emotional functioning. These were then tested in cognitive interviews with patients with nGD (> 12 years of age) and caregivers.
Results
Core registry data elements (n = 138) were identified by seven global clinical experts from Egypt, Germany, Israel, Japan, United Kingdom (UK), and United State (US) and reviewed via online Delphi method by 14 additional clinicians with experience of nGD from six countries and three pharmaceutical representatives. The elements were consistent with those identified via interviews with 10 patients/caregivers with nGD from Japan, Sweden, UK, and US. Key domains identified were demographics, diagnostic information, health status, clinical symptomatology, laboratory testing, treatment, healthcare resource utilization, aids/home improvements, and patient/caregiver burden and quality of life, specifically physical functioning, self-care, daily and social activities, emotional impacts, support services, and caregiver-specific impacts. Nine caregivers and six patients from the US, UK, China, Mexico, Egypt, and Japan participated in the cognitive interviews that informed revisions to ensure that all items are understandable and interpreted as intended.
Conclusions
The comprehensive set of clinical and patient relevant outcomes data, developed collaboratively among all stakeholders, to be reported using GARDIAN will bridge the many gaps in the understanding of nGD and align with regulatory frameworks on real-world data needs.
The Relationship among self-care, disease knowledge and hope in patients with heart failure
Authors: Mehri Zahra , Kheiry Fatemeh , Rakhshan Mahnaz 
Published: April 2023

Abstract
Background: Self-care, disease knowledge, and hope have been considered critical concepts in health-related research. So that all three of them are considered solutions to control heart failure and reduce its complications. The present study was conducted to determine the correlation of self-care with disease knowledge and hope in heart failure patients referred to Imam Reza Clinic, Shiraz, Iran.
Methods: In this correlational and cross-sectional descriptive study, the data of 85 heart failure patients referred to Imam Reza Clinic, Shiraz, Iran in 2021 were examined. Heart failure patients' self-care instruments based on Orem's self-care theory, the Heart Failure Knowledge Scale-Persian version, and the Heart Hope Index-Persian version were used in the present study. To analyze the data, descriptive statistics and the Pearson correlation coefficient were used.
Results: Out of 85 heart failure patients, 56.50% of the samples were male, and 43.50% were female. The mean and standard deviation of the self-care score was 196.54±25.54, the heart failure knowledge score was 4.49±2.59, and the score of hope was reported as 32.41±4.52 in these patients. The analysis of the Pearson correlation coefficient indicates the presence of a positive and significant correlation between "self-care and hope" (r=0.506, p<0.0001), "heart failure knowledge and hope" (r=0.409, p<0.0001), and self-care and heart failure knowledge" (r=0.407, p<0.0001).
Conclusions: Considering the positive and significant correlation between "self-care and heart failure knowledge", "self-care and hope", and "heart failure knowledge and hope", it seems to be necessary for doctors and nurses to pay attention to providing educational programs regarding increasing the heart failure knowledge to improve self-care and hope in heart failure patients.
Seismic evidence for deep hydrothermal circulation and faulting at slow spreading Mid-Atlantic Ridge
Authors: Vaddineni Venkata , Jian Hanchao , Singh Satish 
Published: April 2023

Abstract
Oceanic crust at slow spreading ridges is formed by a combination of magmatic and tectonic processes, where the heat is mined through hydrothermal circulation, but how deep and for how long the hydrothermal circulation occurs remain unknown. Using a combined P-wave and S-wave tomography of ocean bottom seismometer data at the equatorial Mid-Atlantic Ridge, here we show the presence of a high P- to S-wave velocity ratio beneath the ridge axis extending down to 5 km below the seafloor in a 50-km wide zone, indicating the occurrence of deep hydrothermal circulation extending up to ~1.6 Ma. Furthermore, full waveform inversion of coincident multi-channel seismic data shows extensive faulting penetrating down to 4 km depth, allowing the water to circulate deep into the crust, enabling a robust hydrothermal circulation. The presence of a 30-km wide low P-wave velocity anomaly in the mantle underneath reveals the existence of a thermal anomaly providing the heat source, invigorating the deep hydrothermal circulation. These results suggest that most of the heat is mined in the first 1.6 Ma, prior to the initiation of slow conductive plate cooling, the subsequent seafloor subsidence, and the lithospheric evolution.
Error per single-qubit gate below $10^{-4}$ in a superconducting qubit
Authors: Liu Weiyang , Li Zhiyuan , Liu Pei , Zhao Peng , Mi Zhenyu , Xu Hui-Kai , Liang Xuehui , Su Tang , Sun Weijie , Xue Guangming , Zhang Jing-Ning , Jin Yi-Rong , Yu Haifeng 
Published: April 2023

Abstract
Implementing arbitrary single-qubit gates with near perfect fidelity is among the most fundamental requirements in gate-based quantum information processing. In this work, we fabric a transmon qubit with long coherence times and demonstrate single-qubit gates with the average gate error below 10-4, i.e. (7.42±0.04)×10-5 by randomized benchmarking (RB). To understand the error sources, we experimentally obtain an error budget, consisting of the decoherence errors lower bounded by (4.62±0.04)×10-5 and the leakage rate per gate of (1.16±0.04)×10-5. Moreover, we reconstruct the process matrices for the single-qubit gates by the gate set tomography (GST), with which we simulate RB sequences and obtain single-qubit fedlities consistent with experimental results. We also observe non-Markovian behavior in the experiment of long-sequence GST, which may provide guidance for further calibration. The demonstration extends the upper limit that the average fidelity of single-qubit gates can reach in a transmon-qubit system, and thus can be an essential step towards practical and reliable quantum computation in the near future.
High Uric Acid Orchestrates Ferroptosis to Promote Doxorubicin-Induced Cardiomyopathy via ROS-GPX4 Signaling
Authors: Cheng Jidong , Xu Chenxi , Yu Wei , Xie De , Wang Qiang , Chen Binyang , Yang Haiyan , Xi Yuemei , Yu Linqian , Yan Yunbo , Yamamoto Tetsuya , Koyama Hidenori 
Published: April 2023

Abstract
Background: Doxorubicin (DOX) is a chemotherapeutic drug that induces cardiotoxicity known as doxorubicin -induced cardiomyopathy(DIC). Studies have confirmed that DOX can cause cardiac damage via ferroptosis. High uric acid (HUA), as a pro-oxidant, participates in the pathophysiology of cardiovascular disease. Epidemiological studies suggest elevated uric acid levels can have detrimentaleffects on cardiovascular disease. However, the effect of hyperuricemia in a specific type of cardiomyopathy, DIC, is unclear. It is unknown if HUA exacerbates DIC and if the tumor patients with hyperuricemia will aggravate the cardiac side effects of DOX.
Methods: In uricase knockout (Uox-/-) mice, we explored the effect of HUA on DOX-induced cardiotoxicity, including cardiac function, pathomorphology, and its mechanism.
Results: We demonstrated that Uox-KO mice accelerated the development of DIC, causing significantly impaired cardiac function and myocardial fibrosis. Meanwhile, the mitochondrial morphology was destroyed, the lipid peroxidation products increased in number and the antioxidant function was weakened. In addition, we evaluated the effects of ferrostatin-1 (Fer-1), the ferroptosis inhibitor. Myocardial damage can be reversed by the Fer-1 treatment caused by HUA combined with DOX treatment. Benzbromarone, a UA-lowering drug, decreases myocardial fibrosis and ferroptosis by alleviating hyperuricemia in Uox-KO mice by DOX administration. In vitro, we observed that the activity of cardiomyocytes treated with HUA combined with DOX decreased significantly, and lipid reactive oxygen species (ROS) increased significantly. Afterwards, we demonstrated that HUA can promote oxidative stress in DOX, characterised by increased mitochondrial ROS, and down-regulate protein levels of glutathione peroxidase 4 (GPX4). N-acetyl-L-cysteine, an antioxidant, inhibits the process by which HUA promotes DOX-induced ferroptosis by increasing the GPX4 expression.
Conclusions: Our data suggested that HUA promotes the DIC. And HUA promotes DOX-induced ferroptosis by increasing oxidative stress and down-regulating GPX4. It is implied that tumor patients with hyperuricemia may increase cardiac side effects when taking DOX during chemotherapy treatment.
Metabolomics in vitreous fluid of retinal detachment associated with choroidal detachment patients
Authors: Yang Lufei , Luo Shasha , Chen Yanghao , Gong Xuechun , Wu Zhifeng 
Published: April 2023

Abstract
Background
Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is a complex type of retinal detachment. This disease is characterized by high difficulty of operation and poor prognosis. However, the pathogenesis is still unclear. The purpose of this study was to analyze the changes of metabolites and metabolic pathways in vitreous fluid of RRDCD patients.
Method
Using ultra-high-performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry method, metabolites in the vitreous fluid of RRDCD and idiopathic epimacular membrane (IEMM) patients were analyzed. A total of 28 samples were analyzed to compare the significance of different metabolites between these groups.
Results
We identified 135 different metabolites between the RRDCD and IEMM groups (VIP > 1, P value < 0.05). Compared with the IEMM group, the level of sphingomyelin, adenosine, L-palmitoylcarnitine and 4,7,10,13,1 6,19-docosahexaenoic acid up-regulated in RRDCD group. A series of lipid metabolites were up-regulated in RRDCD group. The main metabolic pathways involved were protein digestion and absorption, ABC transporters, aminoacyl-tRNA biosynthesis, central carbon metabolism in cancer and sphingolipids metabolism.
Conclusions
Our results suggest that the up-regulation of sphingomyelin and abnormal sphingolipids metabolism may induce cell migration and death after retinal or choroidal detachment. And then, it may induce intraocular inflammation and damage visual function. The accumulated L-palmitoylcarnitine and fatty acid metabolites may damage the energy pathway of retina, and aggravate the death of photoreceptors after hypoxia. These results provide clues for studying the mechanism, treatment and prognosis of RRDCD.