bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in children’s and adolescents’ dietary intake after the implementation of Chile’s law of food labeling, advertising and sales in schools: a longitudinal study
Authors: Fretes Gabriela , Corvalán Camila , Reyes Marcela , Taillie Lindsey Smith , Economos Christina D. , Wilson Norbert L.W. , Cash Sean B. 
Published: April 2023

Abstract
Background
In June 2016, a comprehensive food policy was implemented in Chile that included front-of-package warning labels on key nutrients of concern (total sugars, added saturated fats, sodium, and calories), child-directed food advertisement bans, and school regulations. The policy was implemented in 3 phases from 2016 to 2019 and the primary objective was to improve children’s food environments. This study’s objective was to assess changes in child and adolescent intake of key nutrients of concern (total sugars, saturated fats, and sodium) at school after the initial implementation of Chile’s Law of Food Labeling and Advertisement.

Methods
Longitudinal study of 349 children from the Food Environment Chilean Cohort (FECHIC) and 294 adolescents from the Growth and Obesity Cohort Study (GOCS). Data were from single 24-hour dietary recalls collected from 2016 to 2019. Fixed-effects models stratified by school, home, and other locations compared nutrient consumption in each year to consumption at the pre-policy 2016 baseline. Nutrient intakes are expressed as percent of total energy.

Results
Compared to 2016 (pre-policy), total sugars consumed by children at school decreased 4.5 [-8.0, -0.9] percentage points (pp) and 11.8 [-15.4, -8.3] pp in 2018 and 2019 respectively. In 2019, children’s saturated fats and sodium intake at school also decreased (1.1 [-1.9, -0.2] pp and 10.3 [-18.1, -2.5] mg/100 kcal respectively). Likewise, in adolescents, total sugars and saturated fats consumed at school decreased in 2018 (5.3 [-8.4, -2.2] pp and 1.5 [-2.7, -0.3] pp respectively). However, consumption of key nutrients of concern at other locations increased after implementation of the policy.

Conclusions
After initial implementation of Chile’s Labeling Law, intake of most key nutrients of concern significantly declined at school. However, we found evidence of compensatory behavior in out-of-school settings. Further research is needed to evaluate what other actions are needed to impact overall diets in the long term both at schools and out of school.
MiR-146b-5p/SEMA3G regulates epithelial-mesenchymal transition in clear cell renal cell carcinoma
Authors: Tang Mengxi , Xiong Tao 
Published: April 2023

AbstractObjectiveThe primary purpose was to unveil how the miR-146b-5p/SEMA3G axis works in clear cell renal cell carcinoma (ccRCC).MethodsccRCC dataset was acquired from TCGA database, and target miRNA to be studied was further analyzed using survival analysis. We performed miRNA target gene prediction through the database, and those predicted miRNAs were intersected with differential mRNAs. After calculating the correlation between miRNAs and mRNAs, we completed the GSEA pathway enrichment analysis on mRNAs. MiRNA and mRNA expression was examined by qRT-PCR. Western blot was introduced to detect SEMA3G, MMP2, MMP9 expression, epithelial-mesenchymal transition (EMT) marker proteins, and Notch/TGF-β signaling pathway-related proteins. Targeted relationship between miRNA and mRNA was validated using a dual-luciferase test. Transwell assay was employed to assess cell migration and invasion. Wound healing assay was adopted for evaluation of migration ability. The effect of different treatments on cell morphology was observed by a microscope.ResultsIn ccRCC cells, miR-146b-5p was remarkably overexpressed, yet SEMA3G was markedly less expressed. MiR-146b-5p was capable of stimulating ccRCC cell invasion, migration and EMT, and promoting the transformation of ccRCC cell morphology to mesenchymal state. SEMA3G was targeted and inhibited via miR-146b-5p. MiR-146b-5p facilitated ccRCC cell migration, invasion, morphology transforming to mesenchymal state and EMT process by targeting SEMA3G and regulating Notch and TGF-β signaling pathways.ConclusionMiR-146b-5p regulated Notch and TGF-β signaling pathway by suppressing SEMA3G expression, thus promoting the growth of ccRCC cells, which provides a possible target for ccRCC therapy and prognosis prediction.
The lost balloon at midnight: a case report reveals the inevitability of heart team existence
Authors: Elbayomi Mohamed , Weyand Michael , Nooh Ehab , Harig Frank 
Published: April 2023

Abstract
Background
Dislodgement of a coronary stent-balloon catheter during percutaneous coronary intervention (PCI) is rare but is a life-threatening complication.

Case summary
A 57- year-old male presented with a non-ST elevation myocardial infarction (NSTEMI). Coronary angiography revealed total thrombotic occlusion of the Right coronary artery (RCA). Following the balloon dilatation of the RCA and while trying to retrieve the balloon catheter, the balloon was dislodged from the catheter shaft and entrapped in the coronary vessel. Under cardiopulmonary bypass, with antegrade cardioplegic arrest, the balloon was extracted through a coronary arteriotomy. Right coronary revascularization was done with reversed saphenous vein graft (SVG).

Discussion
Given the variety of equipment that can be retained in the coronary artery and the multitude of mechanisms by which it may be entrapped, there are no straightforward techniques applicable to all situations. Specific guidelines or recommendations on properly managing these potentially life-threatening complications do not exist. However, the most crucial issue in the management of these cases is the hemodynamic status of the patient as well as the coronary flow in the vessel with entrapped device or stent. In our case, the RCA was retrogradely perfused from the left coronary artery, which provided time to transfer the patient to cardiovascular surgical backup.
Pleural abrasion versus apical pleurectomy for primary spontaneous pneumothorax: a systematic review and Meta-analysis
Authors: Chang Jaewon , Ratnaraj Vignesh , Fu Vincent , Jiang Michael , Peri Varun , Nguyenhuy Minhtuan , Antippa Phillip 
Published: April 2023

Abstract
Background
Surgical approach is the most effective treatment for primary spontaneous pneumothorax. The two most widely adopted surgical methods are mechanical abrasion and apical pleurectomy, in addition to bullectomy. We performed a systematic review and meta-analysis to examine which technique is superior in treating primary spontaneous pneumothorax.

Methods
PubMed, MEDLINE and EMBASE databases were searched for studies published between January 2000 to September 2022 comparing mechanical abrasion and apical pleurectomy for treatment of primary spontaneous pneumothorax. The primary outcome was pneumothorax recurrence. Secondary outcomes included post-operative chest tube duration, hospital length of stay, operative time and intra-operative of blood loss.

Results
Eight studies were eligible for inclusion involving 1,613 patients. There was no difference in the rate of pneumothorax recurrence between pleural abrasion and pleurectomy (RR: 1.34; 95% CI: 0.94 to 1.92). However, pleural abrasion led to shorter hospital length of stay (MD: -0.25; 95% CI: -0.51 to 0.00), post-operative chest tube duration (MD: -0.30; 95% CI: -0.56 to -0.03), operative time (MD: -13.00; 95% CI -15.07 to 10.92) and less surgical blood loss (MD: -17.77; 95% CI: -24.36 to -11.18).

Conclusion
Pleural abrasion leads to less perioperative patient burden and shorter hospital length of stay without compromising the rate of pneumothorax recurrence when compared to pleurectomy. Thus, pleural abrasion is a reasonable first choice surgical procedure for management of primary spontaneous pneumothorax.
Flexible and efficient handling of nanopore sequencing signal data with slow5tools
Authors: Samarakoon Hiruna , Ferguson James M. , Jenner Sasha P. , Amos Timothy G. , Parameswaran Sri , Gamaarachchi Hasindu , Deveson Ira W. 
Published: April 2023

AbstractNanopore sequencing is being rapidly adopted in genomics. We recently developed SLOW5, a new file format with advantages for storage and analysis of raw signal data from nanopore experiments. Here we introduce slow5tools, an intuitive toolkit for handling nanopore data in SLOW5 format. Slow5tools enables lossless data conversion and a range of tools for interacting with SLOW5 files. Slow5tools uses multi-threading, multi-processing, and other engineering strategies to achieve fast data conversion and manipulation, including live FAST5-to-SLOW5 conversion during sequencing. We provide examples and benchmarking experiments to illustrate slow5tools usage, and describe the engineering principles underpinning its performance.
Study protocol for a randomized controlled trial of Proportional Assist Ventilation for Minimizing the Duration of Mechanical Ventilation: the PROMIZING study
Authors: Bosma Karen J. , Martin Claudio M. , Burns Karen E. A. , Mancebo Cortes Jordi , Suárez Montero Juan Carlos , Skrobik Yoanna , Thorpe Kevin E. , Amaral Andre Carlos Kajdacsy-Balla , Arabi Yaseen , Basmaji John , Beduneau Gaëtan , Beloncle Francois , Carteaux Guillaume , Charbonney Emmanuel , Demoule Alexandre , Dres Martin , Fanelli Vito , Geagea Anna , Goligher Ewan , Lellouche François , Maraffi Tommaso , Mercat Alain , Rodriguez Pablo O. , Shahin Jason , Sibley Stephanie , Spadaro Savino , Vaporidi Katerina , Wilcox M. Elizabeth , Brochard Laurent ,   On behalf of the Canadian Critical Care Trials Group and,   the REVA Network
Published: April 2023

Abstract
Background
Proportional assist ventilation with load-adjustable gain factors (PAV+) is a mechanical ventilation mode that delivers assistance to breathe in proportion to the patient’s effort. The proportional assistance, called the gain, can be adjusted by the clinician to maintain the patient’s respiratory effort or workload within a normal range. Short-term and physiological benefits of this mode compared to pressure support ventilation (PSV) include better patient-ventilator synchrony and a more physiological response to changes in ventilatory demand.

Methods
The objective of this multi-centre randomized controlled trial (RCT) is to determine if, for patients with acute respiratory failure, ventilation with PAV+ will result in a shorter time to successful extubation than with PSV. This multi-centre open-label clinical trial plans to involve approximately 20 sites in several continents. Once eligibility is determined, patients must tolerate a short-term PSV trial and either (1) not meet general weaning criteria or (2) fail a 2-min Zero Continuous Positive Airway Pressure (CPAP) Trial using the rapid shallow breathing index, or (3) fail a spontaneous breathing trial (SBT), in this sequence. Then, participants in this study will be randomized to either PSV or PAV+ in a 1:1 ratio. PAV+ will be set according to a target of muscular pressure. The weaning process will be identical in the two arms. Time to liberation will be the primary outcome; ventilator-free days and other outcomes will be measured.

Discussion
Meta-analyses comparing PAV+ to PSV suggest PAV+ may benefit patients and decrease healthcare costs but no powered study to date has targeted the difficult to wean patient population most likely to benefit from the intervention, or used consistent timing for the implementation of PAV+. Our enrolment strategy, primary outcome measure, and liberation approaches may be useful for studying mechanical ventilation and weaning and can offer important results for patients.

Trial registration
ClinicalTrials.gov NCT02447692. Prospectively registered on May 19, 2015.
Human landing catches provide a useful measure of protective efficacy for the evaluation of volatile pyrethroid spatial repellents
Authors: Tambwe Mgeni Mohamed , Kibondo Ummi Abdul , Odufuwa Olukayode Ganiu , Moore Jason , Mpelepele Ahmed , Mashauri Rajabu , Saddler Adam , Moore Sarah Jane 
Published: April 2023

Abstract
Background
The human landing catch (HLC) method, in which human volunteers collect mosquitoes that land on them before they can bite, is used to quantify human exposure to mosquito vectors of disease. Comparing HLCs in the presence and absence of interventions such as repellents is often used to measure protective efficacy (PE). Some repellents have multiple actions, including feeding inhibition, whereby mosquitoes may be unable to bite even if they land on a host. A comparison was made between the PE of the volatile pyrethroid spatial repellent (VPSR) transfluthrin determined using a landing method (HLC) and a biting method (allowing the mosquitoes that landed to blood-feed) to evaluate whether HLC is a suitable method for the estimation of the personal PE of a VPSR.

Methods
A fully balanced, two-arm crossover design study was conducted using a 6 × 6 × 2-m netted cage within a semi-field system. Hessian strips (4 m × 0.1 m) treated with a 5-, 10-, 15-, or 20-g dose of transfluthrin were evaluated against a paired negative control for three strains of laboratory-reared Anopheles and Aedes aegypti mosquitoes. Six replicates were performed per dose using either the landing or the biting method. The number of recaptured mosquitoes was analysed by negative binomial regression, and the PEs calculated using the two methods were compared by Bland–Altman plots.

Results
For Anopheles, fewer mosquitoes blood-fed in the biting arm than landed in the landing arm (incidence rate ratio = 0.87, 95% confidence interval 0.81–0.93, P < 0.001). For Ae. aegypti, biting was overestimated by around 37% with the landing method (incidence rate ratio = 0.63, 95% confidence interval 0.57–0.70, P = 0.001). However, the PEs calculated for each method were in close agreement when tested by the Bland Altman plot.

Conclusions
The HLC method led to underestimation of mosquito feeding inhibition as a mode of action of transfluthrin, and there were species- and dose-dependent differences in the relationship between landing and biting. However, the estimated PEs were similar between the two methods. The results of this study indicate that HLC can be used as a proxy for personal PE for the evaluation of a VPSR, especially when the difficulties associated with enumerating blood-fed mosquitoes in a field setting are taken into consideration.

Graphical Abstract
Dysfunction in parkin aggravates inflammatory bone erosion by reinforcing osteoclast activity
Authors: Kim Eun-Young , Kim Ji-Eun , Kim Young-Eun , Choi Bongkun , Sohn Dong Hyun , Park Si-On , Chung Yeon-Ho , Kim Yongsub , Robinson William H. , Kim Yong-Gil , Chang Eun-Ju 
Published: April 2023

Abstract
Background
Parkin dysfunction associated with the progression of parkinsonism contributes to a progressive systemic skeletal disease characterized by low bone mineral density. However, the role of parkin in bone remodeling has not yet been elucidated in detail.

Result
We observed that decreased parkin in monocytes is linked to osteoclastic bone-resorbing activity. siRNA-mediated knockdown of parkin significantly enhanced the bone-resorbing activity of osteoclasts (OCs) on dentin without any changes in osteoblast differentiation. Moreover, Parkin-deficient mice exhibited an osteoporotic phenotype with a lower bone volume accompanied by increased OC-mediated bone-resorbing capacity displaying increased acetylation of α-tubulin compared to wild-type (WT) mice. Notably, compared to WT mice, the Parkin-deficient mice displayed increased susceptibility to inflammatory arthritis, reflected by a higher arthritis score and a marked bone loss after arthritis induction using K/BxN serum transfer, but not ovariectomy-induced bone loss. Intriguingly, parkin colocalized with microtubules and parkin-depleted-osteoclast precursor cells (Parkin−/− OCPs) displayed augmented ERK-dependent acetylation of α-tubulin due to failure of interaction with histone deacetylase 6 (HDAC6), which was promoted by IL-1β signaling. The ectopic expression of parkin in Parkin−/− OCPs limited the increase in dentin resorption induced by IL-1β, accompanied by the reduced acetylation of α-tubulin and diminished cathepsin K activity.

Conclusion
These results indicate that a deficiency in the function of parkin caused by a decrease in parkin expression in OCPs under the inflammatory condition may enhance inflammatory bone erosion by altering microtubule dynamics to maintain OC activity.
Dimethyl itaconate is effective in host-directed antimicrobial responses against mycobacterial infections through multifaceted innate immune pathways
Authors: Kim Young Jae , Park Eun-Jin , Lee Sang-Hee , Silwal Prashanta , Kim Jin Kyung , Yang Jeong Seong , Whang Jake , Jang Jichan , Kim Jin-Man , Jo Eun-Kyeong 
Published: April 2023

Abstract
Background
Itaconate, a crucial immunometabolite, plays a critical role in linking immune and metabolic functions to influence host defense and inflammation. Due to its polar structure, the esterified cell-permeable derivatives of itaconate are being developed to provide therapeutic opportunities in infectious and inflammatory diseases. Yet, it remains largely uncharacterized whether itaconate derivatives have potentials in promoting host-directed therapeutics (HDT) against mycobacterial infections. Here, we report dimethyl itaconate (DMI) as the promising candidate for HDT against both Mycobacterium tuberculosis (Mtb) and nontuberculous mycobacteria by orchestrating multiple innate immune programs.

Results
DMI per se has low bactericidal activity against Mtb, M. bovis Bacillus Calmette–Guérin (BCG), and M. avium (Mav). However, DMI robustly activated intracellular elimination of multiple mycobacterial strains (Mtb, BCG, Mav, and even to multidrug-resistant Mtb) in macrophages and in vivo. DMI significantly suppressed the production of interleukin-6 and -10, whereas it enhanced autophagy and phagosomal maturation, during Mtb infection. DMI-mediated autophagy partly contributed to antimicrobial host defenses in macrophages. Moreover, DMI significantly downregulated the activation of signal transducer and activator of transcription 3 signaling during infection with Mtb, BCG, and Mav.

Conclusion
Together, DMI has potent anti-mycobacterial activities in macrophages and in vivo through promoting multifaceted ways for innate host defenses. DMI may bring light to new candidate for HDT against Mtb and nontuberculous mycobacteria, both of which infections are often intractable with antibiotic resistance.
Sexual dimorphism in chronic respiratory diseases
Authors: Reddy Karosham Diren , Oliver Brian Gregory George 
Published: April 2023

AbstractSex differences in susceptibility, severity, and progression are prevalent for various diseases in multiple organ systems. This phenomenon is particularly apparent in respiratory diseases. Asthma demonstrates an age-dependent pattern of sexual dimorphism. However, marked differences between males and females exist in other pervasive conditions such as chronic obstructive pulmonary disease (COPD) and lung cancer. The sex hormones estrogen and testosterone are commonly considered the primary factors causing sexual dimorphism in disease. However, how they contribute to differences in disease onset between males and females remains undefined. The sex chromosomes are an under-investigated fundamental form of sexual dimorphism. Recent studies highlight key X and Y-chromosome-linked genes that regulate vital cell processes and can contribute to disease-relevant mechanisms. This review summarises patterns of sex differences in asthma, COPD and lung cancer, highlighting physiological mechanisms causing the observed dimorphism. We also describe the role of the sex hormones and present candidate genes on the sex chromosomes as potential factors contributing to sexual dimorphism in disease.
New stochastic fractional integral and related inequalities of Jensen–Mercer and Hermite–Hadamard–Mercer type for convex stochastic processes
Authors: Jarad Fahd , Sahoo Soubhagya Kumar , Nisar Kottakkaran Sooppy , Treanţă Savin , Emadifar Homan , Botmart Thongchai 
Published: April 2023

AbstractIn this investigation, we unfold the Jensen–Mercer ($\mathtt{J-M}$

J
−
M

) inequality for convex stochastic processes via a new fractional integral operator. The incorporation of convex stochastic processes, the $\mathtt{J-M}$

J
−
M

inequality and a fractional integral operator having an exponential kernel brings a new direction to the theory of inequalities. With this in mind, estimations of Hermite–Hadamard–Mercer ($\mathtt{H-H-M}$

H
−
H
−
M

)-type fractional inequalities involving convex stochastic processes are presented. In the context of the new fractional integral operator, we also investigate a novel identity for differentiable mappings. Then, a new related $\mathtt{H-H-M}$

H
−
H
−
M

-type inequality is presented using this identity as an auxiliary result. Applications to special means and matrices are also presented. These findings are particularly appealing from the perspective of optimization, as they provide a larger context to analyze optimization and mathematical programming problems.
Assessing effectiveness of nature-based solution with big earth data: 60 years mangrove plantation program in Bangladesh coast
Authors: Mahmood Riffat , Zhang Li , Li Guoqing 
Published: April 2023

Abstract
Background
In the climate change context, nature-based solution (NBS) is considered one of the effective tools to increase the resilience of socio-ecological system. The concept coincides with the government’s attempts of afforestation and reforestation programs that have been going on for 60 years in Bangladesh. This study, therefore, envisaged understanding how NBS (mangrove afforestation and reforestation) works to promote climate change resilience through the synthetization of remote sensing-based big earth data, statistical tools, and models. The study took the entire coast of Bangladesh except for Sundarbans Reserve Forest and rolled back to 1962 to work on 60 years’ time series data. Declassified CORONA satellite imagery along with Landsat satellite imagery was used, which is the first-ever attempt in the remote sensing-based ecosystem work in Bangladesh.

Results
The study's main innovation is to spatially establish the effectiveness of the NBS. The study critically assessed and estimated stable lands and their socio-economic benefits as part of the effectiveness of the NBS. As part of the NBS-derived benefits in the context of climate change, it estimated the sequestrated carbon in mangrove forests. A significant positive relationship was observed between the increase of mangroves and stable lands. Near about 448,011 ha of agricultural land was stabilized due to the NBS intervention whose economic value is 18,837 million USD. In addition, 29,755.71 kt of carbon have been sequestrated due to NBS program.

Conclusions
The concept of NBS is still in the development stage and very little or no work has been done so far in measuring and labeling the effectiveness of the NBS. Therefore, our study can innovatively contribute to the scientific community to show the effectiveness of the NBS in three domains (social, economic and ecological) in the changing climatic scenario.