bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a deuterated triarylmethyl spin probe for in vivo R2*-based EPR oximetric imaging with enhanced dynamic range
Authors: Kishimoto Shun , Devasahayam Nallathamby , Chandramouli Gadisetti VR , Murugesan Ramachandran , Otowa Yasunori , Yamashita Kota , Yamamoto Kazutoshi , Brender Jeffrey R , Krishna Murali C 
Published: May 2023

AbstractPurposeIn this study, we compared two triarylmethyl (TAM) spin probes, Ox071 and Ox063 for their efficacy in measuring tissue oxygen concentration by R2*-based oximetry in hypoxic and normoxic conditions.MethodsThe R2* dependence with spin probe and oxygen was calibrated using standard phantom solutions at 1, 2, 5, 10 mM spin probe and 0, 2, 5, 10, 21% oxygen concentrations. For a hypoxic model, in vivo imaging of a MIA PaCa-2 tumor implanted in the hind leg of a mouse was performed on successive days by using either Ox071 or Ox063. For normoxic model, renal imaging of healthy athymic mice was performed under similar conditions. The 3D spin density images acquired under three different gradients and reconstructed by single point imaging modality were used for computing the R2 * values.ResultsThe signal intensities of Ox071 were about three times greater in the phantom solutions than Ox063 in the entire pO2range investigated. Although histograms computed from the pO2images of the tumor were skewed towards low pO2levels for both spin probes due to R2* signal loss, more frequency counts in the normoxic region at pO2> 32 mmHg could be detected with Ox071. In the normoxic model in kidney, the high pO2cortex and the low pO2medulla regions were well delineated. The histograms of high-resolution kidney oximetry images using Ox071 were nearly symmetrical and frequency counts were seen up to 55 mmHg which were missed in Ox063 imaging.ConclusionThis study illustrates Ox071 as a better oximetric probe than Ox063 in terms of sensitivity and the pO2dynamic range.
Functional and genetic adaptations contributing toEnterococcus faecalispersistence in the female urinary tract
Authors: Sharon Belle M. , Arute Amanda P. , Nguyen Amber , Tiwari Suman , Bonthu Sri Snehita Reddy , Hulyalkar Neha V. , Neugent Michael L. , Araya Dennise Palacios , Dillon Nicholas A. , Zimmern Philippe E. , Palmer Kelli L. , De Nisco Nicole J. 
Published: May 2023

ABSTRACTEnterococcus faecalisis the leading Gram-positive bacterial species implicated in urinary tract infection (UTI). An opportunistic pathogen,E. faecalisis a commensal of the human gastrointestinal tract (GIT) and its presence in the GIT is a predisposing factor for UTI. The mechanisms by whichE. faecaliscolonizes and survives in the urinary tract (UT) are poorly understood, especially in uncomplicated or recurrent UTI. The UT is distinct from the GIT and is characterized by a sparse nutrient landscape and unique environmental stressors. In this study, we isolated and sequenced a collection of 37 clinicalE. faecalisstrains from the urine of primarily postmenopausal women. We generated 33 closed genome assemblies and four highly contiguous draft assemblies and conducted a comparative genomics to identify genetic features enriched in urinaryE. faecaliswith respect toE. faecalisisolated from the human GIT and blood. Phylogenetic analysis revealed high diversity among urinary strains and a closer relatedness between urine and gut isolates than blood isolates. Plasmid replicon (rep) typing further underscored possible UT-GIT interconnection identifying nine shared rep types between urine and gutE. faecalis. Both genotypic and phenotypic analysis of antimicrobial resistance among urinaryE. faecalisrevealed infrequent resistance to front-line UTI antibiotics nitrofurantoin and fluoroquinolones and no vancomycin resistance. Finally, we identified 19 candidate genes enriched among urinary strains that may play a role in adaptation to the UT. These genes are involved in the core processes of sugar transport, cobalamin import, glucose metabolism, and post-transcriptional regulation of gene expression.IMPORTANCEUrinary tract infection (UTI) is a global health issue that imposes substantial burden on healthcare systems. Women are disproportionately affected by UTI with >60% of women experiencing at least one UTI in their lifetime. UTIs can recur, particularly in postmenopausal women, leading to diminished quality of life and potentially life-threatening complications. Understanding how pathogens colonize and survive in the urinary tract is necessary to identify new therapeutic targets that are urgently needed due to rising rates of antimicrobial resistance. HowEnterococcus faecalis, a bacterium commonly associated with UTI, adapts to the urinary tract remains understudied. Here, we generated a collection of high-quality closed genome assemblies of clinical urinaryE. faecalisisolated from the urine of postmenopausal women that we used alongside detailed clinical metadata to perform a robust comparative genomic investigation of genetic factors that may mediate urinaryE. faecalisadaptation to the female urinary tract.
WITHDRAWN: Effects of antidiabetic drugs on mortality risks in individuals with type 2 diabetes: A prospective cohort study of UK Biobank participants
Authors: Araldi Elisa , Jutzeler Catherine R. , Ristow Michael 
Published: May 2023

Withdrawal StatementThe authors have withdrawn this manuscript owing to having been informed by the UK Biobank Research Development Team that the use of a subset of prescription data in our analyses does not comply with the Control of Patient Information (COPI, 2020) notice.Specifically, ‘these prescription data could only be used for the purpose of COVID-19 research’. While we have analyzed the impact of these prescription drugs on COVID-19 mortality, as contained in the present manuscript, any additional analyses, as also contained within, appear to be incongruent with the COPI notice.We hence abide by the request of UK Biobank Research Development Team to withdraw the entire publication. We apologize for any inconvenience this may cause. It should be noted that the scientific content, methods and validity of the results in the entire publication remain unaffected by both the request and the subsequent retraction.Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.
A scoping review of behavioural science approaches and frameworks for health protection and emergency response
Authors: Zelenka Martin Alice , Weston Dale , Kesten Jo , French Clare 
Published: May 2023

AbstractAimRapid intervention development, implementation and evaluation is required for emergency public health contexts, such as the recent COVID-19 pandemic. A novel Agile Co-production and Evaluation (ACE) framework has been developed to assist this endeavour in future public health emergencies. This scoping review aimed to map available behavioural science resources that can be used to develop and evaluate public health guidance, messaging, and interventions in emergency contexts onto components of ACE: rapid development and implementation, co-production with patients or the public including seldom heard voices from diverse communities, and inclusion of evaluation.MethodsA scoping review methodology was used. Searches were run on MEDLINE, EMBASE, PsychInfo, and Google, with search terms covering emergency response and behavioural science. Papers published since 2014 and which discussed a framework or guidance for using behavioural science in response to a public health emergency, were included. A narrative synthesis was conducted.ResultsSeventeen records were included in the synthesis. The records covered a range of emergency contexts, the most frequent of which were COVID-19 (n=7) and non-specific emergencies (n=4). One record evaluated existing tools, six proposed new tools, and ten described existing tools. Commonly used tools included the Behavioural Change Wheel, Capability, Opportunity, and Motivation Behaviour model (COM-B model) and social identity theory. Three records discuss co-production with the target audience and consideration of diverse populations. Four records incorporate rapid testing, evaluation, or validation methods. Six records state that their tool is designed to be implemented rapidly. No records cover all components of ACE.ConclusionWe recommend that future research explores how to create guidance involving rapid implementation, co-production with patients or the public including seldom-heard voices from diverse communities, and evaluation.
Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1
Authors: Lorković Zdravko J. , Klingenbrunner Michael , Cho Chung Hyun , Berger Frédéric 
Published: May 2023

AbstractInduction of DNA damage triggers rapid phosphorylation of the histone H2A.X (γH2A.X). In animals, mediator of DNA damage checkpoint 1 (MDC1) binds γH2A.X through a tandem BRCA1 carboxyl-terminal (tBRCT) domain and mediates recruitment of downstream effectors of DNA damage response (DDR). However, readers of this modification in plants have remained elusive. We report that fromArabidopsisBRCT domain proteome, BCP1-4 proteins with tBRCT domains are involved in DDR. Through its tBRCT domain BCP4 binds γH2A.Xin vitroand localizes to DNA damage-induced foci in an H2A.X dependent manner. BCP4 also contains a domain that interacts directly with NBS1 and thus acts as a functional counterpart of MDC1. We also show that BCP1, that contains two tBRCT domains, co-localizes with γH2A.X but it does not bind γH2A.X suggesting functional similarity with human PAXIP1. A phylogenetic analysis supports that PAXIP1 and MDC1 in metazoa and their plant counterparts evolved independently from common ancestors with tBRCT domains. Collectively, our study reveals missing components and provides mechanistic and evolutionary insights into plant DDR.
Tissue-intrinsic Wnt signals antagonize Nodal-driven AVE differentiation
Authors: Schumacher Sina , Fernkorn Max , Marten Michelle , Kim Yung Su , Bedzhov Ivan , Schröter Christian 
Published: May 2023

SummaryThe anterior-posterior axis of the mammalian embryo is laid down by the anterior visceral endoderm (AVE), an extraembryonic signaling center that is specified within the visceral endoderm. Current models posit that AVE differentiation is promoted globally by epiblast-derived Nodal signals, and spatially restricted by a BMP gradient established by the extraembryonic ectoderm. Here, we report spatially restricted AVE differentiation in bilayered embryo-like aggregates made from mouse embryonic stem cells that lack an extraembryonic ectoderm. Notably, clusters of AVE cells also form in pure visceral endoderm cultures upon activation of Nodal signaling, indicating that tissue-intrinsic factors restrict AVE differentiation. We identify Wnt signaling as a tissue-intrinsic factor that antagonizes AVE-inducing Nodal signals. Together, our results suggest that interactions between epiblast and visceral endoderm alone enable local AVE differentiation in the absence of graded BMP signals. This may be a flexible solution for axis patterning in a wide range of embryo geometries.
Crosstalk between the plasma membrane and cell-cell adhesion maintains epithelial identity for correct polarised cell divisions
Authors: Hosawi Manal M. , Cheng Jiaoqi , Fankhaenel Maria , Przewloka Marcin R. , Elias Salah 
Published: May 2023

AbstractPolarised epithelial cell divisions represent a fundamental mechanism for tissue maintenance and morphogenesis; their dysregulation often leads to developmental disorders and cancer. Morphological and mechanical changes in the plasma membrane influence the organisation and crosstalk of microtubules and actin at the cell cortex, thereby regulating the mitotic spindle machinery and chromosome segregation. Yet, the precise mechanisms linking plasma membrane remodelling to cell polarity and cortical cytoskeleton dynamics to ensure accurate execution of mitosis in mammalian epithelial cells remain poorly understood. Here we experimentally manipulated the density of mammary epithelial cells in culture, which led to several mitotic defects. We found that perturbation of cell-cell adhesion integrity impairs the dynamics of the plasma membrane during mitosis, affecting the shape and size of mitotic cells and resulting in defects in mitosis progression and generating daughter cells with aberrant cytoarchitecture. In these conditions, F-actin-astral microtubule crosstalk is impaired leading to mitotic spindle misassembly and misorientation, which in turn contributes to chromosome mis-segregation. Mechanistically, we identify the S100 Ca2+-binding protein A11 (S100A11) as a key membrane-associated regulator that forms a complex with E-cadherin and LGN to coordinate plasma membrane remodelling with E-cadherin-mediated cell adhesion and LGN-dependent mitotic spindle machinery. We conclude that plasma membrane-mediated maintenance of mammalian epithelial cell identity is crucial for correct execution of polarised cell divisions, genome maintenance and safeguarding tissue integrity.
Fascin-induced bundling protects actin filaments from disassembly by cofilin
Authors: Chikireddy Jahnavi , Lengagne Léana , Borgne Rémi Le , Wioland Hugo , Romet-Lemonne Guillaume , Jégou Antoine 
Published: May 2023

ABSTRACTActin filament turnover plays a central role in shaping actin networks, yet the feedback mechanism between network architecture and filament assembly dynamics remains unclear. The activity of ADF/cofilin, the main protein family responsible for filament disassembly, has been mainly studied at the single filament level. Here, we report that fascin, by crosslinking filaments into bundles, strongly slows down filament disassembly by cofilin. We show that this is mainly due to a slower nucleation of the first cofilin clusters, which occurs up to 100-fold slower on large bundles compared to single filaments. In contrast, severing at cofilin cluster boundaries is unaffected by fascin bundling. After the nucleation of an initial cofilin cluster on a filament of a bundle, we observe the local removal of fascin. Surprisingly, the nucleation of cofilin clusters on adjacent filaments is highly enhanced, locally. We propose that this inter-filament cooperativity in cofilin binding arises from the local propagation of the cofilin-induced change in helicity from one filament to the other filaments of the bundle. Taken together, these observations reveal the molecular events explaining why, despite inter-filament cooperativity, fascin crosslinking protects actin filaments from cofilin-induced disassembly. These findings highlight the important role played by crosslinkers in organizing actin networks and modulating the activity of other regulatory proteins.
Native extracellular matrix promotes human neuromuscular organoid morphogenesis and function
Authors: Auletta Beatrice , Rossi Lucia , Cecchinato Francesca , Barbato Gilda , Lauroja Agnese , Chiolerio Pietro , Cecconi Giada , Maghin Edoardo , Easler Maria , Raffa Paolo , Angiolillo Silvia , Qin Wei , Calabrò Sonia , Villa Chiara , Gagliano Onelia , Laterza Cecilia , Cacchiarelli Davide , Cescon Matilde , Giomo Monica , Torrente Yvan , Luni Camilla , Piccoli Martina , Elvassore Nicola , Urciuolo Anna 
Published: May 2023

AbstractHuman neuromuscular organoids (NMOs) derived from induced pluripotent stem cells (hiPSCs) hold a great potential to study (dys)functional human skeletal muscle (SkM) in vitro. The three-dimensional (3D) self-assembly of NMOs leads to the generation of spheroids, whose 3D organization cannot be controlled. Indeed, proper development, maturation and function of the innervated SkM require a well-defined multiscale 3D organization of the cells in a tissue-specific extracellular matrix (ECM) context. We hypothesized that extracellular structural imprinting along with hiPSC small-molecule-based differentiation could provide self-assembly guidance driving NMO morphogenesis and promoting the maturation and function of the human neuronal-coupled SkM in vitro models. We found that SkM ECM, provided as decellularized skeletal muscle, is able to reproducibly guide the morphogenesis of differentiating hiPSC toward multiscale structured tissue-like NMOs (t-NMOs). T-NMOs show contractile activity and possess functional neuromuscular junctions (NMJs), with mature neuromuscular system upon 30 days of hiPSC differentiation. We found that t-NMO could mimic altered muscle contraction upon administration of neurotoxins that act at NMJ level. Finally, we used hiPSCs derived from patients affected by Duchenne Muscular Dystrophy (DMD) to produce DMD t-NMOs that, upon neuronal stimulation, were able to mimic the altered SkM contractility and calcium dynamics typical of the disease. Altogether, our data confirm the ability of t-NMO platform to model in vitro human neuromuscular system (patho)physiology.
The effects of innovation types and customer participation on organizational performance in complex services
Authors: Samuelsson Peter 
Published: May 2023

Purpose
This study aims to explain the effects of different types of innovations on organizational performance in terms of firms’ external effectiveness and internal efficiency. The study examines the interrelationship of technical and nontechnical innovations in complex services and the mediating effect of customer participation on the relationship between innovation type and organizational performance.

Design/methodology/approach
The study draws on a neo-Schumpeterian model for innovation to examine the complex service setting of healthcare provision. Data from Statistics Sweden, containing 38 hospitals and 242 primary care units in Sweden, provided the study's results.

Findings
The findings show the importance of combining different types of innovations in complex services, demonstrating a mediating effect of nontechnical innovation on both the relationship between technical innovations and external effectiveness and internal efficiency. Moreover, the results show that customer participation has a positive mediating effect for technical innovation and nontechnical innovation on external effectiveness. However, there is no such significant effect on internal efficiency.

Research limitations/implications
The findings are based on self-assessment data, which has inherent limitations. The innovation data used were cross-sectional, which may lack reliability (although self-assessed data counter this risk to some extent).

Practical implications
Managers should pursue both technical and nontechnical innovations for gains in external effectiveness and internal efficiency. However, complex services call for technical innovations to be accompanied by nontechnical innovations to support positive effects. The results cause a dilemma for managing customer participation in complex services. As the results show customer participation resulting in external effectiveness, they also fail to establish an effect on internal efficiency.

Originality/value
The primary contribution is to add to the knowledge of different types of innovation in complex services by demonstrating their interdependent effects on both external effectiveness and internal efficiency. Furthermore, the study tests and advances the mediating effect of customer participation in complex services on organizational performance.
Burnout effect on working mothers in leadership positions during the COVID-19 lockdown
Authors: Mucharraz y Cano Yvette , Davila Ruiz Diana , Cuilty Esquivel Karla 
Published: May 2023

Purpose
This study aims to understand how the recent COVID-19 pandemic impacted burnout levels among working mothers in leadership positions and how income and work schemes play an important role in their burnout.

Design/methodology/approach
Data were collected from 961 working mothers and fathers in leadership positions in Mexico under different work schemes during the COVID-19 lockdown. Snowball sampling was used in this study. The Maslach Burnout Inventory General Survey was distributed online, using the burnout scale, with income and work schemes as categorical variables.

Findings
Burnout levels among working mothers in leadership positions were higher than those among working fathers. The hybrid work scheme (i.e. working from home combined with working from office) lessens burnout in working mothers, contributing both theoretically and empirically to better understanding burnout levels of mothers in leadership positions.

Practical implications
The findings can encourage human resource areas to reflect on the overexertion and work stress of mothers in leadership positions, and potential support resources can be provided to motivate them and retain their talent.

Originality/value
The introduction of the notion of lockdown in a conceptual model to observe its interaction with burnout and hybrid work schemes (i.e. working from the office and home) has rarely been discussed in existing literature. The impact, especially for working mothers in leadership positions, must be thus carefully considered while dealing with future crises, thereby helping to develop policies and processes accordingly.
Managerial ownership and financial distress: evidence from the Chinese stock market
Authors: Shan Yuan George , Troshani Indrit , Wang Jimin , Zhang Lu 
Published: May 2023

PurposeThis study investigates the convergence-of-interest and entrenchment effects on the relationship between managerial ownership and financial distress using evidence from the Chinese stock market. It also analyzes whether the relationship is mediated by research and development (R&D) investment.Design/methodology/approachUsing a dataset consisting of 19,059 firm-year observations of Chinese listed companies in the Shanghai and Shenzhen Stock Exchanges between 2010 and 2020, this study employs both piecewise and curvilinear models.FindingsThe results indicate that managerial ownership has a negative association with firm financial distress in both the low (below 12%) and high (above 18%) convergence-of-interest regions of managerial ownership, suggesting that managerial ownership in this region may contribute to improve firm financial status. Meanwhile, managerial ownership has a positive association with firm financial distress in the entrenchment region (12–18%), implying that managerial ownership in the entrenchment region may contribute to impair firm financial status. Furthermore, the results show that R&D investment mediates the association between managerial ownership and financial distress.Originality/valueThis study is the first to provide evidence of a nonlinear relationship between managerial ownership and financial distress, and identify the entrenchment region in the Chinese setting.
Do a non-core worker's procedural justice concerns influence their engagement in helping behavior? A multi-method study
Authors: Farhan Mohammed , Krejci Caroline C. , Cantor David E. 
Published: May 2023

PurposeThe purpose of this research is to examine how a change in team dynamics impacts an individual's motivation to engage in helping behavior and operational performance.Design/methodology/approachAn online vignette experiment and a hybrid discrete event and agent-based simulation model are used.FindingsStudy findings demonstrate how a non-core worker's perception of team dynamics influence engagement in helping behavior and system performance.Originality/valueThis study provides a further understanding on how team members react to changes in team processes. This study theorizes on how an individual team member responds to fairness concerns. This study also advances our understanding of the critical importance of helping behavior in a retail logistics setting. This research illustrates how the theory of strategic core and procedural justice literature can be adopted to explain team dynamics in supply chain management.