bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carcinoma-associated fibroblast-derived lysyl oxidase-rich extracellular vesicles mediate collagen crosslinking and promote epithelial-mesenchymal transition via p-FAK/p-paxillin/YAP signaling
Authors: Liu Xue , Li Jiao , Yang Xuesong , Li Xiaojie , Kong Jing , Qi Dongyuan , Zhang Fuyin , Sun Bo , Liu Yuehua , Liu Tingjiao 
Published: August 2023

AbstractCarcinoma-associated fibroblasts (CAFs) are the main cellular components of the tumor microenvironment and promote cancer progression by modifying the extracellular matrix (ECM). The tumor-associated ECM is characterized by collagen crosslinking catalyzed by lysyl oxidase (LOX). Small extracellular vesicles (sEVs) mediate cell-cell communication. However, the interactions between sEVs and the ECM remain unclear. Here, we demonstrated that sEVs released from oral squamous cell carcinoma (OSCC)-derived CAFs induce collagen crosslinking, thereby promoting epithelial-mesenchymal transition (EMT). CAF sEVs preferably bound to the ECM rather than being taken up by fibroblasts and induced collagen crosslinking, and a LOX inhibitor or blocking antibody suppressed this effect. Active LOX (αLOX), but not the LOX precursor, was enriched in CAF sEVs and interacted with periostin, fibronectin, and bone morphogenetic protein-1 on the surface of sEVs. CAF sEV-associated integrin α2β1 mediated the binding of CAF sEVs to collagen I, and blocking integrin α2β1 inhibited collagen crosslinking by interfering with CAF sEV binding to collagen I. CAF sEV-induced collagen crosslinking promoted the EMT of OSCC through FAK/paxillin/YAP pathway. Taken together, these findings reveal a novel role of CAF sEVs in tumor ECM remodeling, suggesting a critical mechanism for CAF-induced EMT of cancer cells.
Posterior cervical congenital dermal sinus tract: case report and review of literature
Authors: Vokshoor Amir , Jajj Harseerat , Grunwald Tiffany , Kolker Steven , Petros Jack 
Published: August 2023

Abstract
Background and importance
Congenital dermal sinus tract (DST) is a rare spinal dysraphism characterized by a persistent tract lined by epithelial cells, beginning at the epidermis and terminating in deeper tissue layers. With 1% of all congenital DST cases found in the cervical region, only 4% of all cases are diagnosed after the age of 20.

Clinical presentation
In this case, a 65-year-old woman with a congenital DST at the cervical level presented with symptoms of neck and some arm pain, suboccipital headaches, and unique external characteristics. Neck Disability Index and visual analog scale were used to assess the patient’s preoperative and postoperative pain, and quality of life. Patient underwent an operative intervention, where the DST was surgically removed followed by interlaminar decompression at C1–C2, excision of the epidural component, and biopsy followed by plastic surgical repair. Pathology analysis indicated a squamous epithelial-lined sinus tract interacting with the dura. Most notably, a meningothelial proliferation with associated psammomatous calcifications was identified, similar to a meningioma.

Conclusion
A review of literature was conducted to further discuss clinical and radiological presentation as well as to document the novel appearance of this congenital DST. As one of the oldest cases of DST, it demonstrated unusual pathological characteristics with a meningothelial proliferation, compatible with meningioma, reported at the epidural level.
Valve turning towards on-cycle in cobalt-catalyzed Negishi-type cross-coupling
Authors: Luo Xu , Yang Dali , He Xiaoqian , Wang Shengchun , Zhang Dongchao , Xu Jiaxin , Pao Chih-Wen , Chen Jeng-Lung , Lee Jyh-Fu , Cong Hengjiang , Lan Yu , Alhumade Hesham , Cossy Janine , Bai Ruopeng , Chen Yi-Hung , Yi Hong , Lei Aiwen 
Published: August 2023

AbstractLigands and additives are often utilized to stabilize low-valent catalytic metal species experimentally, while their role in suppressing metal deposition has been less studied. Herein, an on-cycle mechanism is reported for CoCl2bpy2 catalyzed Negishi-type cross-coupling. A full catalytic cycle of this kind of reaction was elucidated by multiple spectroscopic studies. The solvent and ligand were found to be essential for the generation of catalytic active Co(I) species, among which acetonitrile and bipyridine ligand are resistant to the disproportionation events of Co(I). Investigations, based on Quick-X-Ray Absorption Fine Structure (Q-XAFS) spectroscopy, Electron Paramagnetic Resonance (EPR), IR allied with DFT calculations, allow comprehensive mechanistic insights that establish the structural information of the catalytic active cobalt species along with the whole catalytic Co(I)/Co(III) cycle. Moreover, the acetonitrile and bipyridine system can be further extended to the acylation, allylation, and benzylation of aryl zinc reagents, which present a broad substrate scope with a catalytic amount of Co salt. Overall, this work provides a basic mechanistic perspective for designing cobalt-catalyzed cross-coupling reactions.
Bottlenose dolphins are sensitive to human attentional features, including eye functionality
Authors: Davies James R. , Garcia-Pelegrin Elias 
Published: August 2023

AbstractThe ability to attribute attentional states to other individuals is a highly adaptive socio-cognitive skill and thus may have evolved in many social species. However, whilst humans excel in this ability, even chimpanzees appear to not accurately understand how visual attention works, particularly in regard to the function of eyes. The complex socio-ecological background and socio-cognitive skill-set of bottlenose dolphins (Tursiopssp.), alongside the specialised training that captive dolphins typically undergo, make them an especially relevant candidate for an investigation into their sensitivity to human attentional states. Therefore, we tested 8 bottlenose dolphins on an object retrieval task. The dolphins were instructed to fetch an object by a trainer under various attentional state conditions involving the trainer’s eyes and face orientation: ‘not looking’, ‘half looking’, ‘eyes open’, and ‘eyes closed’. As the dolphins showed an increased latency to retrieve the object in conditions where the trainer’s head and eyes cued a lack of attention to the dolphin, particularly when comparing ‘eyes open’ vs ‘eyes closed’ conditions, we demonstrate that dolphins can be sensitive to human attentional features, namely the functionality of eyes. This study supports growing evidence that dolphins possess highly complex cognitive abilities, particularly those in the social domain.
Metabolomic analysis of follicular fluid from women with Hashimoto thyroiditis
Authors: Bastos Diana Caroline da Silva , Chiamolera Maria Izabel , Silva Renata Elen , Souza Maria Do Carmo Borges de , Antunes Roberto Azevedo , Souza Marcelo Marinho , Mancebo Ana Cristina Allemand , Arêas Patrícia Cristina Fernandes , Reis Fernando M. , Lo Turco Edson Guimarães , Bloise Flavia Fonseca , Ortiga-Carvalho Tania M. 
Published: August 2023

AbstractHashimoto thyroiditis is an autoimmune disease characterized by hypothyroidism and a high level of anti-thyroid autoantibodies. It has shown to negatively impact female fertility; however, the mechanisms are unclear. Ovarian follicular fluid appears to be the key to understanding how Hashimoto thyroiditis affecst fertility. Thus, we aimed to evaluated the metabolic profile of follicular fluid and antithyroid autoantibody levels in the context of Hashimoto thyroiditis. We collected follicular fluid from 61 patients, namely 38 women with thyroid autoantibody positivity and 23 women as negative controls, undergoing in vitro fertilization treatment. Follicular fluid samples were analyzed using metabolomics, and thyroid autoantibodies were measured. Fifteen metabolites with higher concentrations in the follicular fluid samples from Hashimoto thyroiditis were identified, comprising five possible affected pathways: the glycerophospholipid, arachidonic acid, linoleic acid, alpha-linolenic acid, and sphingolipid metabolism pathways. These pathways are known to regulate ovarian functions. In addition, antithyroglobulin antibody concentrations in both serum and follicular fluid were more than tenfold higher in women with Hashimoto thyroiditis than in controls. Our data showed that the metabolic profile of follicular fluid is altered in women with Hashimoto thyroiditis, suggesting a potential mechanistic explanation for the association of this disease with female infertility.
Experimental and techno-economic investigation of industrial para-xylene plant revamping to produce meta-xylene
Authors: Khosravi-Nikou MohammadReza , Shahmoradi Ali , Shariati Ahmad , Hajilari Meysam , Malek-Mahmoudi Mahsa , Jafari Nemat-Allah , Sheikh-Nezhad Abdollah 
Published: August 2023

AbstractIn this research, the feasibility study of revamping of simulated moving bed for paraxylene separation in ELUXYL process to produce meta-xylene using industrial Ba-faujasite exchanged adsorbent by changing operating condition (temperature and pressure) were examined experimentally and theoretically. Two different mixed-xylene feed cases (with and without presence of para-xylene) were considered. Different sets of temperature and pressure were evaluated with the help of equilibrium and dynamic experiments to obtain optimum operating condition in a favor of separation of meta-xylene. Results confirm that in the presence of para-xylene in a feed, selectivity of the adsorbent could not change towards meta-xylene. But, in the absence of para in some pressure and temperature meta-xylene was more selective than ortho and ethylbenzene. Finally, by the means of statistical experimental design method the results of all experiments were compered and an optimum temperature and pressure were found. Breakthrough experiment in optimum condition showed MX/OX and MX/EB selectivity as 1.83 and 1.15, respectively. Furthermore, the design and simulation of the real industrial SMB plant was performed in Aspen Chromatography and HYSYS software to evaluate the real performance of MX separation. Simulation results showed the final purification in SMB plant can be reached to 83.91%. At the end, for the aim of promoting purity by considering extra distillation towers the purity of meta-xylene was achieved by 96%. The economical investigation showed that by considering 700$/ton for feed supplied, the process can be satisfactory from economical point of view.
Identification of potent anti-Candida metabolites produced by the soft coral associated Streptomyces sp. HC14 using chemoinformatics
Authors: Abdella Bahaa , Abdella Mohamed , ElSharif Hafed A. , ElAhwany Amani M. D. , El‑Sersy Nermeen A. , Ghozlan Hanan A. , Sabry Soraya A. 
Published: August 2023

AbstractCandida albicans is the most common pathogen responsible for both spontaneous and recurrent candidiasis. The available treatment of Candida infections has several adverse effects, and the development of new drugs is critical. The current study looked at the synthesis of anti-Candida metabolites by Streptomyces sp. HC14 recovered from a soft coral. Using the Plackett Burman design, the medium composition was formulated to maximize production. Using GC–MS, the compounds have been identified, and a cheminformatics approach has been used to identify the potential source of activity. The compounds that showed high potential for activity were identified as pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)-3 and di-n-octyl based on their docking score against the cytochrome monooxygenase (CYP51) enzyme in Candida albicans. As a result of their discovery, fewer molecules need to be chemically synthesized, and fermentation optimization maximizes their synthesis, providing a strong foundation for the development of novel anti-Candida albicans agents.
Automatic identification method of bridge structure damage area based on digital image
Authors: Wang Jinchao , Liu Houcheng , Han Zengqiang , Wang Yiteng 
Published: August 2023

AbstractIt is of great scientific and practical value to use effective technical means to monitor and warn the structural damage of bridges in real time and for a long time. Traditional image recognition network models are often limited by the lack of on-site images. In order to solve the problem of automatic recognition and parameter acquisition in digital images of bridge structures in the absence of data information, this paper proposes an automatic identification method for bridge structure damage areas based on digital images, which effectively achieves contour carving and quantitative characterization of bridge structure damage areas. Firstly, the digital image features of the bridge structure damage area are defined. By making full use of the feature that the pixel value of the damaged area is obviously different from that of the surrounding image, an image pre-processing method of the structure damaged area that can effectively improve the quality of the field shot image is proposed. Then, an improved Ostu method is proposed to organically fuse the global and local threshold features of the image to achieve the damaged area contour carving of the bridge structure surface image. The scale of damage area, the proportion of damage area and the calculation rule of damage area orientation are constructed. The key inspection and characteristic parameter diagnosis of bridge structure damage area are realized. Finally, test and analysis are carried out in combination with an actual project case. The results show that the method proposed in this paper is feasible and stable, which can improve the damage area measurement accuracy of the current bridge structure. The method can provide more data support for the detection and maintenance of the bridge structure.
Improving the efficiency of 4A-zeolite synthesized from kaolin by amine functionalization for CO2 capture
Authors: Bahmanzadegan Fatemeh , Pordsari Mahyar Ashourzadeh , Ghaemi Ahad 
Published: August 2023

AbstractThis study focuses on optimizing the CO2 adsorption capacity of 4A-zeolite synthesized from kaolin by employing structural modifications through impregnation with tetraethylenepentamine (TEPA) and diethanolamine (DEA). Various analytical techniques were utilized to evaluate the effectiveness of these modifications. Design expert software and response surface methodology (RSM) was employed for data analysis and operational variable optimization, leading to improved CO2 adsorption performance of the modified zeolites. The adsorption capacity of the modified zeolites was assessed under different temperatures, pressures, and amine concentrations using a test device. The optimal adsorption capacity of 4A-DEA adsorbent is found to be 579.468 mg/g, with the optimal operational variables including a temperature of 25.270 °C, pressure of 8.870 bar, and amine concentration of 11.112 wt%. The analysis shows that the adsorption process involves both physisorption and chemisorption, and the best kinetic model is the fractional-factor model.
Alternating L4 loop architecture of the bacterial polysaccharide co-polymerase WzzE
Authors: Wiseman Benjamin , Widmalm Göran , Högbom Martin 
Published: August 2023

AbstractLipopolysaccharides such as the enterobacterial common antigen are important components of the enterobacterial cell envelope that act as a protective barrier against the environment and are often polymerized by the inner membrane bound Wzy-dependent pathway. By employing cryo-electron microscopy we show that WzzE, the co-polymerase component of this pathway that is responsible for the length modulation of the enterobacterial common antigen, is octameric with alternating up-down conformations of its L4 loops. The alternating up-down nature of these essential loops, located at the top of the periplasmic bell, are modulated by clashing helical faces between adjacent protomers that flank the L4 loops around the octameric periplasmic bell. This alternating arrangement and a highly negatively charged binding face create a dynamic environment in which the polysaccharide chain is extended, and suggest a ratchet-type mechanism for polysaccharide elongation.
Drone swarm strategy for the detection and tracking of occluded targets in complex environments
Authors: Amala Arokia Nathan Rakesh John , Kurmi Indrajit , Bimber Oliver 
Published: August 2023

AbstractDrone swarms can achieve tasks via collaboration that are impossible for single drones alone. Synthetic aperture (SA) sensing is a signal processing technique that takes measurements from limited size sensors and computationally combines the data to mimic sensor apertures of much greater widths. Here we use SA sensing and propose an adaptive real-time particle swarm optimization (PSO) strategy for autonomous drone swarms to detect and track occluded targets in densely forested areas. Simulation results show that our approach achieved a maximum target visibility of 72% within 14 seconds. In comparison, blind sampling strategies resulted in only 51% visibility after 75 seconds and 19% visibility in 3 seconds for sequential brute force sampling and parallel sampling respectively. Our approach provides fast and reliable detection of occluded targets, and demonstrates the feasibility and efficiency of using swarm drones for search and rescue in areas that are not easily accessed by humans, such as forests and disaster sites.
Circulating extracellular vesicles containing S100A9 reflect histopathology, immunophenotype and therapeutic responses of liver metastasis in colorectal cancer patients
Authors: Tsamchoe Migmar , Lazaris Anthoula , Kim Diane , Krzywon Lucyna , Bloom Jessica , Mayer Thomas , Petrillo Stephanie K , Dejgaard Kurt , Gao Zu-Hua , Rak Janusz , Metrakos Peter 
Published: August 2023

Abstract
Background
Metastasis is the principal cause of cancer treatment failure and an area of dire diagnostic needs. Colorectal cancer metastases to the liver (CRCLMs) are predominantly classified into desmoplastic and replacement based on their histological growth patterns (HGPs). Desmoplastic responds well to current treatments, while replacement HGP has a poor prognosis with low overall survival rates.

Methods
We hypothesised that complex cellular response underlying HGPs may be reflected in the proteome of circulating extracellular vesicles (EVs). EV proteomics data was generated through LC-MS/MS and analysed with Maxquant and Perseus. To validate the S100A9 signature, ELISA was performed, and IHC and IF were conducted on tissue for marker detection and colocalization study.

Results
Plasma EV proteome signature distinguished desmoplastic from the replacement in patients with 22 differentially expressed proteins, including immune related markers. Unsupervised PCA analysis revealed clear separation of the two lesions. The marker with the highest confidence level to stratify the two HGPs was S100A9, which was traced in CRCLM lesions and found to colocalize with macrophages and neutrophils. EV-associated S100A9 in plasma may reflect the innate immunity status of metastatic lesions and their differential therapeutic responses.

Conclusion
Plasma EV-derived S100A9 could be useful in personalising therapy in patients with CRCLM.
Task‐oriented feature hallucination for few‐shot image classification
Authors: Wu Sining , Gao Xiang , Hu Xiaopeng 
Published: August 2023

AbstractData hallucination generates additional training examples for novel classes to alleviate the data scarcity problem in few‐shot learning (FSL). Existing hallucination‐based FSL methods normally train a general embedding model first by applying information extracted from base classes that have abundant data. In those methods, hallucinators are then built upon the trained embedding model to generate data for novel classes. However, these hallucination methods usually rely on general‐purpose embeddings, limiting their ability to generate task‐oriented samples for novel classes. Recent studies have shown that task‐specific embedding models, which are adapted to novel tasks, can achieve better classification performance. To improve the performance of example hallucination for tasks, a task‐oriented embedding model is used in the proposed method to perform task‐oriented generation. After the initialization, the hallucinator is finetuned by applying a task‐oriented embedding model with the guidance of a teacher–student mechanism. The proposed task‐oriented hallucination method contains two steps. An initial embedding network and an initial hallucinator are trained with a base dataset in the first step. The second step contains a pseudo‐labelling process where the base dataset is pseudo‐labelled using support data of the few‐shot task and a task‐oriented fine‐tuning process where the embedding network and hallucinator are adjusted simultaneously. Both the embedding network and the hallucinator are updated with the support set and the pseudo‐labelled base dataset using knowledge distillation. The experiments are conducted on four popular few‐shot datasets. The results demonstrate that the proposed approach outperforms state‐of‐the‐art methods with 0.8% to 4.08% increases in classification accuracy for 5‐way 5‐shot tasks. It also achieves comparable accuracy to state‐of‐the‐art methods for 5‐way 1‐shot tasks.
Ashwagandha
Authors: Chopra Ananda Samir 
Published: August 2023

SummaryAshwagandha birgt großes Potenzial im therapeutischen Einsatz bei neurologischen und psychovegetativen Erkrankungen sowie zur Stärkung des Immunsystems. Die verfügbaren klinischen Daten sind jedoch limitiert, sodass intensive Untersuchungen zur Bestätigung der Wirksamkeit und Sicherheit nötig sind.
A Novel Neuroimaging Phenotype in the Pediatric Paroxysmal Kinesigenic Dyskinesia
Authors: Sinha Rahul , Hosur Bharat , Singh Sonali , Kamila Gautam , Meena Ankit Kumar 
Published: August 2023

AbstractParoxysmal kinesigenic dyskinesia (PKD) is a rare disorder characterized by recurrent attacks of hyperkinetic movements which can be isolated or associated with benign infantile seizures as part of the infantile convulsions with choreoathetosis syndrome. We present a case of hyperkinetic movement disorder in the form of choreoathetosis, ballismus, dystonia triggered by sudden movements with a past history of benign infantile convulsions in a 12-year-old girl. The contrast-enhanced brain and spine magnetic resonance imaging showed bilaterally symmetric superior cerebellar cytotoxic edema sparing the vermis with swollen cerebellar foliae. Whole-exome sequencing identified a homozygous frameshift duplication NM_145239.3(PRRT2):c.649dupC(p.Arg217Profs*8) in the PRRT2 gene. This case report highlights the frameshift duplication in the PRRT2 gene and rare neuroimaging findings which further expand the phenotypic characteristics of PKD in children.