bluuebunny/crossref_metadata_2025_split huggingface dataset:
The pathogenic role of the EGFR/STAT5 signaling pathway in synovial fibroblasts of patients with rheumatoid arthritis
Authors: Wang Wang chenyu , Liang Liang chuancai , Xing Xing hengte , Gong Gong ziheng , Qiu Qiu Bo , Hao Hao yarong 
Published: March 2023

Abstract
Objective

To investigate the role of EGFR/STAT5 signaling pathway in the pathogenesis of rheumatoid arthritis (RA).

Method

Identifying the differentially expressed genes (DEGs) with bioinformatics analysis. Rheumatoid arthritis fibroblast-like synovial cells (RA-FLS) were collected from patients clinically and cultured in vitro. EGFR and STAT expression levels were detected in tissues using immunohistochemistry(IHC). Moreover the expression of downstream proteins of the EGFR/STAT5 pathway(IL-2, IL-15, Bcl-2, and Bcl-xL)were quantified in RA-FLS cells using western blot.

Result

It was found that EGFR expression was significantly different in RA synovium, and immunohistochemistry confirmed that EGFR and STAT5 was significantly expressed in RA synovium. Additionally, in vitro experiments using RA-FLS uncovered that protein expression levels of IL-2, IL-15, Bcl-2, and Bcl-xL were significantly increased compared to normal FLS. More importantly, the protein expression levels of IL-2, IL-15, Bcl-2, and Bcl-xL were significantly decreased after EGFR inhibitor use, while an opposite trend was observed when EGFR agonists were utilized.

Conclusion

Inhibiting EGFR/STAT5 signaling pathway results in the apoptosis of RA-FLS and prohibits the occurrence of RA-FLS inflammatory response, which can be used as a therapeutic target for RA.
Effects of different intervention methods on autophagic protein and inflammatory factors in testicular tissue of obese rats
Authors: Chen Lin , Li Ningchuan 
Published: March 2023

Abstract
Objective
To investigate the relationship between inflammation and autophagy in high-fat obese rats by applying three intervention methods (aerobic exercise/orlistat/food restriction).
Methods
Male SD rats were randomly divided into normal control group (NC group, n = 10) and high fat model group (HC group, n = 67). 40 rats (n = 58) were randomly divided into high fat control group (HC group, n = 10), high fat exercise group (HE group, n = 10), high fat orlistat group (HO group, n = 10), high fat diet group (HR group, n = 10). After 8 weeks, the weight and body length of rats were measured, and Lee's index and body fat ratio were calculated; Four blood lipids in rat serum were detected by Elisa method. Inflammatory factors IL-6 and TNF-α in testicular tissue were detected by Elisa method. The expression levels of autophagic protein AKT and mTOR mRNA in testis were detected by RT-PCR.
Results
(1) Compared with NC group, the body weight and Lee's index of rats in HC group increased significantly (P < 0.01), and the body fat ratio increased significantly (P < 0.05); The body weight in HR group was significantly lower than that in HE and HO groups (P < 0.01). (2) Compared with NC group, the levels of TC, TG and LDL-C in serum of HC group increased significantly(P < 0.01), while the level of HDL-C decreased significantly(P < 0.01); Compared with HC group, the levels of TC and TG in HR and HE groups decreased significantly(P < 0.01), while the level of HDL-C in HR group increased significantly(P < 0.01). (3) The level of IL-6 in testicular tissue of HC group was significantly higher than that of NC group(P < 0.01). Compared with HC group, the level of IL-6 in HE and HR groups decreased significantly(P < 0.01), and the level of TNF-α in HR group decreased significantly(P < 0.05). (4) The expression level of AKT mRNA in testicular tissue of HC group was significantly higher than that of NC group(P < 0.05). Compared with HC, the expression level of each index in HO and HR groups was significantly increased(P < 0.01), and the AKT mRNA level in HE group was significantly higher than that in HC group(P < 0.05).
Conclusions
(1) The obese rat model reproduced in this study is successful. The three intervention methods can inhibit the weight growth of rats and effectively improve the dyslipidemia, of which the effect of food restriction intervention is the most significant. (2) The three intervention methods can effectively alleviate the inflammatory level in the testis of obese rats; The effect of aerobic exercise and food restriction intervention on reducing the level of inflammation is better than that of orlistat drug intervention. (3) Compared with food restriction intervention, exercise intervention has a greater impact on AKT and mTOR mRNA expression levels. The level of inflammatory factors is negatively correlated with the expression of autophagy related proteins, and autophagy level has a high degree of tissue specificity.
Towards understanding the prospects of select macrophytes as potential fish feed
Authors: Naseem Shahida , Bhat Sami Ullah , Gani Adil , Bhat Farooz Ahmad 
Published: March 2023

Abstract
Aquaculture provides high-quality protein reasonably at cheap cost. But the intensive aquaculture is facing expensive feeding cost because the key aquadiet ingredient- fishmeal is becoming very costly. In this scenario application of aquatic plants as an alternative for fishmeal has the potential to revolutionize aquafarming in meeting protein demand globally. Macrophytes grow abundantly in all water bodies and are considered as a nuisance in most of eutrophic aquatic systems. In the current study the nutritional profile of five aquatic plants, namely Azolla cristata, Ceratophyllum demersum, Nelumbo nucifera, Nymphaea mexicana and Trapa natans, collected from the Dal Lake, Kashmir was evaluated. The proximate analysis was carried out as per standard methods of association of official analytical chemists (AOAC) and mineral analysis by atomic absorption spectroscopy. Results show that the crude protein lies in the range of 16–24% with Nymphaea mexicana showing the highest (24.13%) and Ceratophyllum demersum showing the lowest (16.07%) crude protein content. The crude lipid content ranges between 4–8% whereas ash content lies in the range of 11- 37.74% with C. demersum showing the highest (37.74%). Among the investigated macrophytes, the highest levels of potassium, magnesium, iron, manganese, cadmium and copper were recorded in C. demersum. Azolla cristata was rich in sodium and zinc, and Nelumbo nucifera in cobalt. Omega-6 and omega-3 fatty acids were present in all the investigated macrophytes except C. demersum. Our findings revealed that macrophytes are rich sources of fatty acids, protein and minerals besides their readily availability and high productivity and it is argued here in this paper they can prove as viable candidates for aquafeed in the future.
Ultrasound-based nomogram for predicting lateral cervical lymph node metastasis in thyroid papillary carcinoma
Authors: Chen Wenqin , Cang Qing , Lu Wenjuan , Yuan Ya , Li Lu , Deng Hongyan , Ye Xinhua 
Published: March 2023

Abstract
Objective: To explore the risk factors of lateral cervical lymph node metastasis(LLNM) in thyroid papillary carcinoma (PTC), establish a nomograph prediction model to predict the probability of LLNM before operation, reduce the risk of recurrence, avoid secondary surgery, and achieve the goal of accurate treatment.
Methods: In this retrospective study, 242 patients were enrolled and randomly divided into two groups according to the ratio of 7:3. The preoperative clinical data of patients (including ultrasonic characteristics and selected clinical characteristics) were collected, and least absolute shrinkage and selection operator (Lasso) in the training cohort was used to screen the influencing factors. Based on the results of multivariable logistic regression, the nomograph model is established, and its performance is evaluated by its identification, calibration and clinical usefulness in training and validation cohorts.
Results: This study showed that age>45 years (OR 0.340; 95%CI 0.129-0.856), abnormal serum TG (OR 3.626; 95%CI 1.397-10.187), thyroid tumer location1 (OR 0.250; 95%CI 0.083-0.702), US-reported positive central cervical lymph node (CLN) (OR 3.116; 95%CI 1.152-8.601), and US-reported positive LLN (OR 8.426; 95%CI 3.192-23.779) were independent risk factors for LLNM of PTC. The nomogram constructed based on the above factors has good resolution and calibration in predicting the LLNM of PTC. The Concordance index (C-index) of the experimental group and the validation group are 0.880 and 0.937, respectively.
Conclusion: The nomogram based on ultrasound and clinical features constructed in this study has good performance in non-invasive predicting LLNM of PTC and is expected to promote clinical decision-making.
Traditional foods and herbal preparations to improve the perceived insufficiency of breast milk among lactating women in the Dire Dawa Administration, eastern Ethiopia, 2022
Authors: Mohammed Aminu , Amsalu Bezabih 
Published: March 2023

Abstract
Background
Perceptions of insufficient breast milk quantity and the use of some traditional food items and local herbs to increase it are a common phenomenon worldwide, particularly in low-resource countries like Ethiopia. But the food items and herbs used are variable and inconsistent in different areas. So far, studies are scarce nationally and in the study area, and therefore, this study was aimed at assessing the use of traditional food items and local herbs for the perceived benefit of breast milk increment and its associated factors.
Methodology
: A community-based mixed study was conducted from October 01–30, 2022 in the Dire Dawa administration, eastern Ethiopia. A systematic random sampling technique was used to select 722 participants for the quantitative study and purposive sampling for the qualitative study. Data were collected through face-to-face interviews using a pre-tested structured questionnaire and semi-structured questions for focus group discussion. Quantitative data were entered and cleaned by Epi DATA (Version 3.1) and analyzed using SPSS (Version 22). A P-value < 0.25 at bivariate to select variables for multivariate and ≤ 0.05 at multivariate with 95% confidence intervals was considered statistically significant. Qualitative data were analyzed using thematic analysis using open-source software.
Results
722 women for quantitative and nine focus group discussants for qualitative were included and the overall traditional food and local herbs use to increase perceived insufficient breast milk was 32.8% (95% CI: 29.4–36.6%). A higher age group (AOR = 2.31, 95%CI: 1.37–3.90), rural residence (AOR = 1.98, 95%CI: 1.21–3.23), no formal education (AOR = 2.25, 95%CI: 1.16–4.36), primary level education (AOR = 2.02, 95%CI: 1.18–3.46), presence of medical illness (AOR = 4.73, 95%CI: 1.96–11.43), prior use experience (AOR = 2.81, 95% CI: 1.78–4.46) and a lack of postnatal counseling (AOR = 1.80, 95% CI: 1.20–2.70) were associated with a higher likelihood of traditional food and local herbs use. Traditional food items and local herbs were the main identified themes in the qualitative results.
Conclusion
The extent of traditional food and local herbs use to increase perceived insufficient breast milk was a common practice and associated with women’s age, education, resident area, presence of medical illness, prior use experience, and a lack of postnatal counseling. Stakeholders were recommended to increase postnatal counseling and awareness while taking women's residence and education level into account, as well as conduct additional community-based research.
A Single Feed Graphene-based Circularly Polarized Antenna for THz Applications
Authors: Varshney Arun Kumar , Pathak Nagendra Prasad , Sircar Debabrata 
Published: March 2023

Abstract
In this paper, a frequency reconfigurable, circularly polarized graphene-based antenna has been designed. The antenna consists of a truncated graphene patch and single series cross-aperture coupled feeding. Two orthogonal modes are generated by the sequential rotation of the electromagnetic signal on the graphene patch through the cross aperture in ground plane. The frequency reconfigurability can be achieved by varying the external dc bias voltage at the graphene patch. The proposed antenna can resonate in the broad range of frequencies from 1.22 to 1.44 THz at chemical potential from 0.5 to 1.0 eV. In this range of chemical potential, the axial ratio of the antenna is less than 3 dB. The maximum gain of antenna has been achieved up to 4.6 dBi with 62% radiation efficiency at the chemical potential of 1 eV.
Does the Size of Nanohydroxyapatite Associated With Anionic Collagen Scaffolds Interferes With Osteoblasts Bioactivity?
Authors: Freire Gildênio Estavam , Carvalho Elayne Valério , Veras Mariana de Oliveira Viana , Costa Deiziane Viana Silva , Rebouças Conceição da Silva Martins , Silva Guilherme Ferreira Maciel , Morais Maria Luana Gaudencio dos Santos , Góes Paula , Chaves Anderson Valério , Fechine Pierre Basílio Almeida , Brito Gerly Anne de Castro , Ferreira Júlio César Góes , Leitão Renata Ferreira de Carvalho 
Published: March 2023

Abstract
Objectives
We aimed to evaluate the effect of nanohydroxyapatite morphology and its interaction with anionic collagen on osteoblast activity.
Materials and Methods
Murine osteoblasts were incubated with a commercial collagen scaffold (as a control) or collagen-nanohydroxyapatite scaffolds (Col-HANP) for 24 and 48 hours for viability and proliferation assessments by MTT and Ki67 immunofluorescence, respectively. The hydroxyapatite nanoparticles were synthesized in three different morphologies/sizes (labeled as Col-HANP 0h, as Col-HANP 2h, and as Col-HANP 5h) as a function of the hydrothermal synthetic approach. Osteoblast's activity was investigated by bone alkaline phosphatase activity (ALP) and Von Kossa mineralization assays. For biocompatibility evaluation, the scaffolds were implanted subcutaneously in the dorsum of male Wistar rats for 7 and 15 days.
Results
The incubation of cells with Col-HANP 5h for 48h resulted in a significant increase in their proliferation and activity. The implantation of Col-HANP 5h in the subcutaneous tissue presented decreased recruitment of inflammatory cells and IL-1β levels on day 7, as well as an increase in collagen synthesis on day 15 compared to collagen and control groups.
Conclusions
The significant effects on osteoblasts proliferation and activity illustrate the potential application of Col-HANP 5h scaffold as a promising strategy for bone tissue engineering.
Partitions into Segal--Piatetski--Shapiro sequences
Authors: Li Ya-Li , Zhou Nian Hong 
Published: March 2023

Abstract
Note: Please see pdf for full abstract with equations.
Let κ be any positive real number and m ∈ N ∪ {∞} be given. Let pκ,m(n) denote the number of partitions of n into the parts from the Segal-Piatestki-Shapiro sequence ([ℓκ])ℓ∈N with at most m possible repetitions. In this paper, we establish some asymptotic formulas of Hardy{Ramanujan type for pκ,m(n). As a necessary step in the proof, we prove that the Dirichlet series ζκ(s) = ∑n≥1[nκ]−s can be continued analytically beyond the imaginary axis except for simple poles at s = 1/κ − j, (0 ≤ j < 1/κ, j ∈ Z).
Mathematics Subject Classification 2020| Primary 11P82; Secondary 11N37; 05A17
Cerebral Hemodynamic Changes Associated with Neuroinflammation under Seawater Immersion Hypothermia
Authors: Ye Dan , Hou Pengwei , Zhang Hao , Lin Yinghong , Lin Long , Yang Yang , Xu Yongjun , Wang Shousen 
Published: March 2023

Abstract
Traumatic brain injury (TBI) combined with seawater immersion hypothermia (SIH) is one of the main causes of death and disability in shipwrecks. However, the impact of SIH on brain injury is still unclear. In this study, we used 8-week-old C57BL/6 mice to establish a controlled cortical impact model and simulate the hypothermia caused by seawater immersion after mice with TBI fell into seawater. The vital signs of the mouse model were continuously measured in the early stage of modeling, and the CBF of the mouse was recorded using laser scatter contrast imaging before and after modeling and after 6 and 24h. The cerebral cortex of 24h mice was collected for histopathological examination and enzyme-linked immunosorbent assay. The behavioral functions were assessed by behavioral analysis. The results showed that brain parenchymal injury was exacerbated during hypothermia in mice with TBI. CBF was significantly reduced in the hypothermic group compared with the normothermic group, and the levels of some inflammatory cytokines significantly increased and were correlated with low CBF. Overall, the results of this study suggested a correlation between inflammatory progression and low CBF, indicating that venous blood flow might be used for assessing neuroinflammatory progression after hypothermic trauma.
Targeting of Focal Adhesion Kinase enhances the immunogenic cell death of PEGylated liposome doxorubicin to optimize therapeutic responses of immune checkpoint blockade
Authors: Zhang Baoyuan , Li Ning , Gao Jiaming , Zhao Yuxi , Jiang Jun , Xie Shuang , Zhang Cuiping , Zhang Qingyu , Liu Leo , Wang Zaiqi , Ji Dongmei , Wu Linying , Ren Ruibao 
Published: March 2023

Abstract
Immune checkpoint blockade (ICB) is widely considered to exert long-term treatment benefits by activating antitumor immunity. However, many cancer patients show poor clinical responses to ICB due in part to the lack of immunogenic niche. Focal adhesion kinase (FAK) is frequently amplified and acts as an immune modulator across cancer types. However, evidence illustrates that targeting FAK is most effective in combination therapy rather than in monotherapy. Here, we demonstrated that the FAK inhibitor, IN10018, and pegylated liposome doxorubicin (PLD) exerted effective antitumor activity clinically. It is noteworthy that the regimen exhibited response latency and long-lasting effects, outcomes frequently observed in immunotherapy. Our preclinical study confirmed that the 2-drug combination can maximize the immunogenic cell death (ICD) of cancer cells. Thereby, this approach primed the tumor microenvironment, supplementing it with sufficient tumor-infiltrating lymphocytes (TILs) to activate antitumor immunity. The animal studies confirmed that the antitumor effects of ICB can be significantly enhanced by this doublet regimen. Finally, a further clinical trial exhibited that the triplet combination of PD-1 blockade, IN10018, and PLD has already established promising antitumor potency in the real world.
Deep Bug Reports Processing (DBRP): A Systematic Literature Review
Authors: Ahmad Aminu Abdullahi , Yu Lasheng , Kholief Mohamed , Haruna Khalid 
Published: March 2023

Abstract
Many software projects utilize Bug Tracking System (BTS) to manage and process bug reports. Over the years, the number of bug report submissions has increased exponentially with some projects receiving as many as about a hundred submissions daily. Bug report processing (BRP) consists of five key processes: duplicate detection, severity prediction, fix-time prediction, bug triage, and bug localization. Previously, traditional machine learning (ML) algorithms -based models were proposed to automate BRP tasks. However, in recent years deep BRP (DBRP) models were proposed to exploit the ever-increasing bug repositories for automatic extraction of semantic and contextual features of bug reports. Although, some papers reviewed related literature from many perspectives of software maintenance, no existing work comprehensively reviewed the state of DBRP. In this paper, we review the state of DBRP models in the five key BRP tasks. For this, we collect papers from four international databases published between 2015 and 2021. We evaluate the papers with regard to the deep neural networks, text representation models, and bug report features utilized. Finally, we present findings and prospects for future research works in DBRP. Our review analyzes how word embedding, CNNs, LSTMs, attention mechanism are used to represent bug report and source code, model performance and challenges such as data imbalance, feature utilization and complexity.
UoloNet: Based on Multi-tasking Enhanced Small Target Medical Segmentation Model
Authors: Zhang Kejia , Zhang Lan , Pan Haiwei 
Published: March 2023

Abstract
In recent years, UNET and its derivative models have been widely used in medical image segmentation with more superficial structures and excellent segmentation results. Due to the lack of modeling for the overall characteristics of the target, the division tasks of minor marks will produce some discrete noise points, resulting in a decline in model accuracy and application effects. We propose a multi-tasking medical image analysis model UoloNet, a YOLO-based target detection branch is added based on UNET. The shared learning of the two tasks through semantic segmentation and target detection has promoted the model’s mastery of the overall characteristics of the target. In the reasoning stage, merging the two functions of target detection and semantic seg-mentation can effectively remove discrete noise points in the division and enhance the accuracy of semantic segmentation. In the future, the target detection task will be the problem of excessive convergence of semantic segmentation tasks. The model uses CIOU losses instead of IOU losses in YOLO, which further improves the model’s overall accuracy. The effectiveness of the proposed model is verified both in the MRI dataset SEHPI, which we posted and in the public dataset LITS.
The Efficient Activity of Glabridin and its Derivatives Against EGFRmediated
Inhibition of Breast Cancer
Authors: Ghosh Arabinda , Ghosh Debanjana , Mukerjee Nobendu , Maitra Swastika , Das Padmashree , Dey Abhijit , Sharkawi Souty M.Z. , Zouganelis Georgios D. , Alexiou Athanasios , Chaudhari Somdatta Yashwant , Sharma Ritika , Waghmare Sonali Arun , Papadakis Marios , Batiha Gaber El-Saber 
Published: March 2023

Background:
Breast cancer (BC) is one of the most typical causes of cancer
death in women worldwide. Activated epidermal growth factor receptor (EGFR) signaling
has been increasingly associated with BC development and resistance to cytotoxic
drugs. Due to its significant association with tumour metastasis and poor prognosis,
EGFR-mediated signaling has emerged as an attractive therapeutic target in BC. Mainly
in all BC cases, mutant cells over-expresses EGFR. Certain synthetic drugs are already
used to inhibit the EGFR-mediated pathway to cease metastasis, with several phytocompounds
also revealing great chemopreventive activities.

Methods:
This study used chemo-informatics to predict an effective drug from some selected
phytocompounds. The synthetic drugs and the organic compounds were individually
screened for their binding affinities, with EGFR being the target protein using molecular
docking techniques.

Results:
The binding energies were compared to those of synthetic drugs. Among phytocompounds,
Glabridin (phytocompound of Glycyrrhiza glabra) manifested the best dock
value of -7.63 Kcal/mol, comparable to that of the highly effective anti-cancer drug Afatinib.
The glabridin derivatives also exhibited comparable dock values.

Conclusion:
The AMES properties deciphered the non-toxic features of the predicted
compound. Pharmacophore modeling and in silico cytotoxicity predictions also exhibited
a superior result assuring their drug likeliness. Therefore, Glabridin can be conceived as
a promising therapeutic method to inhibit EGFR-mediated BC.