bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS
Authors: Wang Yue , Yang Lin‐Hui , Tong Ling‐Ling , Yuan Li , Ren Bo , Guo Dong‐Sheng 
Published: July 2023

AbstractIntroductionOphiocordyceps gracilis, a type of edible and medicinal fungus, exhibits multiple health‐promoting effects. Due to the scarcity of natural O. gracilis, artificial cultures have been developed as its substitutes. However, lacking comprehension of the metabolite composition of cultures limits its utilisation.ObjectiveThis research aimed to evaluate the nutritional and medicinal value of four cultures of O. gracilis by analysing their metabolite composition. In addition, metabolic pathways in mycelia and fruiting bodies were analysed to explore fruiting body formation mechanism at metabolic level.MethodThe mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis were cultivated in this study. Their metabolite composition was compared using an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry (LC–MS/MS).ResultsPrincipal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS‐DA) showed that the four cultures have noticeable differences in metabolite composition. A total of 612 metabolites were identified, among which 159 metabolites showed significant differences, and these differential metabolites were classified into 13 categories. The metabolites in the fruiting bodies were the most abundant compared with other cultures. However, each culture had its own advantages and significantly accumulates some active metabolites respectively. Pearson's correlation analysed the mutual relationship among metabolites. In addition, seven metabolic pathways were closely related to fruiting body formation, such as “Biosynthesis of plant secondary metabolites”, “amino acids metabolism”, “tricarboxylic acid (TCA) cycle”.ConclusionThis study offered a reference to mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis as health‐promoting functional foods and medicine.
Study of the chlorogenic acid extraction with choline chloride‐based deep eutectic solvents and its non‐covalent interactions analysis
Authors: Herrera‐Beltrán Ilse V. , Méndez‐Torruco Mauricio , Matus Myrna H. , Domínguez Zaira 
Published: July 2023

AbstractThe microwave‐assisted extraction of the main phenolic component of green coffee beans, chlorogenic acid (CGA), was carried out employing deep eutectic solvents based on choline chloride and five different hydrogen bond donors (HBD) in a 1:2 ratio. The best performance for the extraction process of CGA was reached by the mixtures of choline chloride/ethylene glycol and choline chloride/urea. To understand the various interactions between the phenolic compound and the two most efficient deep eutectic solvents, computational calculations were carried out at the density functional theory (DFT) level, as well as Atoms in Molecules (AIM) and Non‐Covalent Interactions (NCIs) analyses. In that way, a variety of hydrogen bond types were found in every structure. Nevertheless, the CGA does not disrupt the hydrogen bond network established between ChCl and the HBD. Among the strongest interactions are those hydrogen bonds between the quinic acid moiety and the ethylene glycol or the urea. In addition, the thermochemistry of the formation of the two main deep eutectic solvents and their corresponding complexes with CGA was calculated, where the formation of urea‐based structures was slightly more effective by ~3 kcal/mol.
Eucalyptus globulus essential oil thin film polymerized by cold plasma on Ti6Al4V: Sterilization effect, antibacterial activity, adhesion, and viability of mesenchymal stem cells
Authors: da Silva Bullmann Matheus , de Castro Victor Velho , Coutinho Daniel Augusto Krieger , Lopes Fernanda Cortez , Maurmann Natasha , Pereira Marcelo B. , Rodrigues Miguel , Pranke Patricia , Ferraz Maria Pia , Lopes Maria Ascensão , Arenas Leliz Ticona , Malfatti Célia de Fraga 
Published: July 2023

AbstractThis work aims to evaluate the characterization, antibacterial activity, and mesenchymal stem cell (MSC) viability in films obtained by cold plasma polymerization, from the essential oil of Eucalyptus globulus on Ti6Al4V alloy. The film presented low roughness values and a contact angle of 85°. The process incorporated a coating with oxygenated functional groups (OH and C═O). The effect of ultraviolet radiation, ethylene oxide, autoclave, and air plasma sterilization on polymer degradation was evaluated. Biofilm formation by Staphylococcus aureus was reduced on the film surface with no toxicity to MSCs. These effects can be attributed to functional groups, combined with the surface roughness and contact angle. These results suggest a potential for the application of this coating in biomaterials.
Validation of in situ diagnostics for the detection of OH and H2O2 in liquids treated by a humid atmospheric pressure plasma jet
Authors: Schüttler Steffen , Jolmes Ludwig , Jeß Emanuel , Tschulik Kristina , Golda Judith 
Published: July 2023

AbstractA humid atmospheric pressure plasma jet was used to treat an aqueous liquid. The transport of hydrogen peroxide and hydroxyl from the plasma to the liquid was analyzed. Two in situ liquid diagnostics for each species were compared and validated. In the case of H2O2, a spectrophotometric approach using ammonium metavanadate and electrochemical sensing based on Prussian blue carbon paste electrodes was applied. Both methods show very good agreement in trends and absolute values. The detection of OH was performed by terephthalic acid (TA) dosimetry and its distribution was visualized by the chemiluminescence of luminol. There, the measurement using TA resembles the luminol measurements and vice versa, and a very good agreement between both methods was found.
Protective effect and possible mechanisms of resveratrol in animal models of osteoporosis: A preclinical systematic review and meta‐analysis
Authors: Lin Fan , Chen Jiaru , Chen Mangmang , Lin Shenglei , Dong Shuangxia 
Published: July 2023

AbstractResveratrol (RES) has extensively been utilized to treat osteoporosis (OP) in animal models. However, the anti‐OP effects of RES have not been tested during clinical application due to the lack of evidence and poor knowledge of the underlying mechanisms. Moreover, there is little preclinical evidence to support the use of RES in the management of OP. In the present paper, we conducted a preclinical systematic review and meta‐analysis to assess the efficacy of RES in animal OP models. The potential mechanisms underlying the efficacy of RES against OP were summarized. The online databases PubMed, CNKI, EMBASE, Wanfang, Web of Science, Chinese Biomedical Literature, Cochrane Library, and Chinese VIP were retrieved from inception to December 2021. The CAMARADES 10‐item quality checklist was utilized to assess the risk of bias of the included studies. STATA 12.0 software was employed to analyze the data. The quality of evidence was assessed using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach. Thirteen studies containing 248 animals were included yielding a mean risk of bias score of 5.54 (range 4–7). The pooled estimates showed that the administration of RES could significantly elevate the bone mineral density (BMD) both at femur (SMD = 2.536; 95% CI = 1.950–3.122; p < 0.001) and lumbar spine (SMD = 1.363; 95% CI = 0894–1.832; p < 0.001), bone volume over total volume (BV/TV) (SMD = 2.543; 95% CI = 2.023–3.062; p < 0.001), trabecular linear density (Tb.N) (SMD = 2.724; 95% CI = 2.186–3.262; p < 0.001) and trabecular thickness (Tb.Th) (SMD = 1.745; 95% CI = 1.294–2.196; p < 0.001), while serum phosphorus (S‐P) (SMD = −2.168; 95% CI = −2.753 to −1.583; p < 0.001) and trabecular separation (Tb.Sp) (SMD = −2.856; 95% CI = −4.218 to −1.494; p < 0.001) were significantly reduced in animal OP models. No significant change in serum calcium (S‐Ca) (SMD = −2.448; 95% CI = −5.255–0.360; p = 0.087) was observed after RES treatment. Furthermore, RES could significantly improve the bone biomechanical indexes: bone maximum load (BML) (SMD = 2.563; 95% CI = 1.827–3.299; p < 0.001) and connectivity density (Conn.D) (SMD = 1.512; 95% CI = 0.909–2.116; p < 0.001) and decrease the structural model index (SMI) (SMD = −2.522; 95% CI = −3.243 to −1.801; p < 0.001). Overall, the present study revealed that RES has huge prospects as a medicine or dietary supplement for the clinical treatment of OP. High‐quality studies with stringent designs and larger sample sizes are warranted to substantiate our conclusion.
Differential health outcomes of the COVID‐19 pandemic among minority populations: An analysis based on Chicago's neighborhoods
Authors: Geletta Simon , Soyemi Kenneth 
Published: July 2023

AbstractBackgroundThe study examines the effects of the COVID‐19 pandemic on different ethnic and racial groups. It aims to investigate the existence or nonexistence of significant variations in COVID‐19 health outcomes among two ethnic and racial minorities that resided in Chicago neighborhoods during the onslaught of the pandemic. Researchers have traditionally studied health disparities by comparing the health of minorities representing “underserved” populations and those with adequate healthcare. This study focuses on the heterogeneity of health outcomes between different minority populations, mainly Black and Hispanic, traditionally considered underserved populations.MethodsThis cross‐sectional study uses secondary data from a public reporting site. The unit of analysis is neighborhood units based on US postal zip codes that are cross‐referenced with the US Census Bureau's zip code tabulation area codes. We used Chicago neighborhood data and applied geographic analyses to describe the patterns of similarities and differences in the outcomes of the COVID‐19 pandemic among neighborhoods with different ethnic and racial minorities residing in them. Using the one‐way analysis of variance technique, we also tested research hypotheses about the COVID‐19 outcome differences and/or similarities among the neighborhoods.ResultsOur findings show that although Hispanic neighborhoods disproportionately carried a higher burden of infection by the disease, the mortality due to the illness or the case fatality rate was not much higher than in the other neighborhoods. In contrast, African American neighborhoods did experience significantly higher case fatality rates—although their infection rate was not statistically significantly higher than the average infection rates of the other Chicago neighborhoods.ConclusionsMinority status creates distinct adverse effects on different minority groups. The patterns of distinct outcomes need to be well understood through further studied and considered by policymakers when health policies are designed to address the impact of health disparities.
Decoherence of Single‐Excitation Entanglement over Duan‐Lukin‐Cirac‐Zoller Quantum Networks Caused by Slow‐Magnetic‐Field Fluctuations and Protection Approach
Authors: Sun Can , Li Ya , Hou Yi‐bo , Wang Min‐jie , Li Shu‐jing , Wang Hai 
Published: July 2023

AbstractAtomic spin‐wave (SW) memory is a building block for quantum repeater. However, due to decoherence caused by atomic motions and magnetic‐field gradients, SW retrieval efficiency decays with storage time. Slow magnetic‐field fluctuations (SMFFs) lead to shot‐to‐shot phase noises of SWs, but have not been considered in previous repeater protocols. Here, a SW model including a homogeneous phase noise caused by SMFFs is developed, and then revealed that such phase noise induces decoherence of single‐excitation entanglement between two atomic ensembles in a repeater link. For verifying this conclusion, single‐excitation entanglement between two SWs in a cold atomic ensemble is experimentally prepared and observed entanglement decoherence induced by SMFFs. Limited by SMFFs, the evaluated lifetime of the single‐excitation entanglement over a repeater link is ≈135 ms, even if the link uses optical‐lattice atoms as nodes. Then an approach that may overcome such limitations and extend this lifetime to ≈1.7 s is presented.
High‐resolution thermal imagery reveals how interactions between crown structure and genetics shape plant temperature
Authors: Olsoy Peter J. , Zaiats Andrii , Delparte Donna M. , Germino Matthew J. , Richardson Bryce A. , Roop Spencer , Roser Anna V. , Forbey Jennifer S. , Cattau Megan E. , Buerki Sven , Reinhardt Keith , Caughlin T. Trevor 
Published: July 2023

AbstractUnderstanding interactions between environmental stress and genetic variation is crucial to predict the adaptive capacity of species to climate change. Leaf temperature is both a driver and a responsive indicator of plant physiological response to thermal stress, and methods to monitor it are needed. Foliar temperatures vary across leaf to canopy scales and are influenced by genetic factors, challenging efforts to map and model this critical variable. Thermal imagery collected using unoccupied aerial systems (UAS) offers an innovative way to measure thermal variation in plants across landscapes at leaf‐level resolutions. We used a UAS equipped with a thermal camera to assess temperature variation among genetically distinct populations of big sagebrush (Artemisia tridentata), a keystone plant species that is the focus of intensive restoration efforts throughout much of western North America. We completed flights across a growing season in a sagebrush common garden to map leaf temperature relative to subspecies and cytotype, physiological phenotypes of plants, and summer heat stress. Our objectives were to (1) determine whether leaf‐level stomatal conductance corresponds with changes in crown temperature; (2) quantify genetic (i.e., subspecies and cytotype) contributions to variation in leaf and crown temperatures; and (3) identify how crown structure, solar radiation, and subspecies‐cytotype relate to leaf‐level temperature. When considered across the whole season, stomatal conductance was negatively, non‐linearly correlated with crown‐level temperature derived from UAS. Subspecies identity best explained crown‐level temperature with no difference observed between cytotypes. However, structural phenotypes and microclimate best explained leaf‐level temperature. These results show how fine‐scale thermal mapping can decouple the contribution of genetic, phenotypic, and microclimate factors on leaf temperature dynamics. As climate‐change‐induced heat stress becomes prevalent, thermal UAS represents a promising way to track plant phenotypes that emerge from gene‐by‐environment interactions.
Influence of Fe on Crystallization Behaviour and Electrical Conductivity of V2O5 ⋅ MoO3 ⋅ CdO ⋅ ZnO Glass Nanocomposite
Authors: Mondal Ajit , Biswas Prosanta , Kumar Mondal Manas , Karmakar Srikanta , Roy Debasish , Bhattacharya Sanjib , Mallik Amit 
Published: July 2023

AbstractThe effect of varying iron content on the crystallization behavior and electrical conductivity mechanism of glass nanocomposite based on the system xFe ⋅ (1‐x) ⋅ (0.3 V2O5 ⋅ 0.2MoO3 ⋅ 0.4CdO ⋅ 0.1ZnO) (x=0.0, 0.05 and 0.1) was investigated by differential scanning calorimetry (DSC), Fourier transforms infrared (FTIR), X‐ray diffraction (XRD), and field emission‐scanning electron microscopy (FESEM). Different crystal phases and the average size of the developed nanocrystallites in the as‐prepared samples have been obtained from the XRD diffraction data. The size of estimated nanocrystallites increases up to Fe (x) content 0.05, after which the size of nanocrystallites decreases as the Fe content in the compositions increases. FESEM micrographs confirm the formation of plate‐like and dendrite nano crystallites throughout the glass matrix. Energy‐dispersive X‐ray spectroscopy (EDX) mapping analysis shows the weight percentage of each constituent element. The dc activation energy (Eσ), as well as activation energy (EH) for hopping frequency of as‐prepared samples, gradually decreases with increasing Fe content whereas AC and DC conductivity gradually increased.
An Ion Selective Electrode Study: Naproxen (NA) Quantification in Pharmaceutical Formulations
Authors: Fatah Sazgar Q. , Karim Wrya O. 
Published: July 2023

AbstractThis study presents fabrication, characterizations and application of an ion‐selective electrode based on naproxen‐sodium tungstate (NA‐ST) ion pairs as a sensor for the quantification of naproxen in a number of pharmaceutical formulations. The ISE is made of the sensor (electrochemical active species or ion pairs) as well as polyvinyl chloride (PVC) and dibutyl phthalate as supporting and plasticizer materials, respectively. The characterizations of the sensor film comprise usage of Fourier transform infrared (FTIR), scanning electron microscope (SEM); in addition to determining the stoichiometric ratio between the components of the sensor using elemental analysis (CHN microanalysis). The analytical evaluation of the sensor film based on selectivity, sensitivity, and Nernstian behavior (55.8±1.1 mV/decade) are shown. The relative high selectivity of sensor towards NA as a target in the presence of several common interferents are exhibited. The ISE possesses a satisfactory sensitivity by recording a detection limit of 0.005 μM. The sensor also experiences a Nernstian behavior during all measurements. The dynamic linear range lies between 0.01 and 100 μM at 25 °C with R2=0.9998. The shelf life is 17 s and longevity lasts four months.
Synthesis andIn vitro‐In silicoEvaluation of Thiazolo‐triazole Hybrids as Anticancer Candidates
Authors: Aggarwal Ranjana , Kumar Prince , Jain Naman , Hooda Mona , Kumar Suresh , Sadana Rachna , Lwanga Robert , Guzman Andrea , Chugh Heerak , Chandra Ramesh 
Published: July 2023

AbstractTo meet the growing need for stable and clinically effective chemotherapeutic agents, herein, we have synthesized a series of acyl‐functionalized thiazolo[3,2‐b]‐[1,2,4]triazole derivatives in highly efficient and regioselective manner. The structure of newly synthesized regioisomeric products was unambiguously characterized by multinuclear 2D‐NMR [(1H−13C) HMBC, (1H−13C) HMQC] spectroscopic data. To assess the anticancer and pharmacokinetic profile of the synthesized compounds, we performedin silico, andex vivobinding studies of compounds with calf‐thymus deoxyribonucleic acid (ct‐DNA) and bovine serum albumin (BSA), respectively, and also tested their efficacy against five human cancer cell lines.viz., MCF‐7 (breast cancer), BT‐474 (breast cancer), A549 (lung cancer), MOLT4 (acute lymphoblastic leukemia), and BxPC3 (pancreatic cancer). Compounds (2‐(4‐chlorophenyl)‐6‐methylthiazolo[3,2‐b][1,2,4]triazol‐5‐yl)(4‐fluorophenyl)methanone6 l, (4‐chlorophenyl)(2‐(4‐chlorophenyl)‐6‐methylthiazolo[3,2‐b][1,2,4]triazol‐5‐yl)methanone6 mand (2‐(4‐chlorophenyl)‐6‐methylthiazolo[3,2‐b][1,2,4]triazol‐5‐yl)(4‐methoxyphenyl) methanone6 qexhibited excellent anticancer potential among the screened derivatives. Furthermore, ex‐vivomechanistic investigations showed the static mode of quenching and moderate bindings between the ligand and biomolecules; DNA (Kq=2.01‐2.24*1012 M−1 s−1) and BSA (Kq=2.25‐.2.72*1012 M−1 s−1). Moreover, fluorescence displacement assay demonstrated a groove binding mode of interaction with ct‐DNA, which was supported by UV‐Visible absorption spectrum, circular dichroism, and viscosity analysis.
Strong Be−Be and Zn−Zn Bonded Aromatic Complexes of Ethyne
Authors: Guha Ankur Kanti 
Published: July 2023

AbstractQuantum chemical calculations reveal that ethyne (C2H2) can be a very good fragment to create strong Be−Be and Zn−Zn interactions. The complexes are thermodynamically stable in which Be2and Zn2fragments act as electron donors towards C2H2fragment acquiring Be2δ+and Zn2δ+states. The partial positive nature of Be2and Zn2fragments made their electronic structure to be described adequately by single reference character. Introduction of electronegative F atom as in C2F2results in shorter Be−Be and Zn−Zn distances. Bonding analyses reveal that all these complexes feature delocalized π bond resulting in aromaticity.
Peptide‐tethered Perylenebisimide Aggregates with Copper Nanoclusters showing Thermoresponsive and Photoresponsive Properties
Authors: Hazra Soumyajit , Bairagi Dipayan , Hazra Niladri , Nandi Arun Kumar , Banerjee Arindam 
Published: July 2023

AbstractA centrally located dipeptide(P) covalently attached to two perylene bisimide units [(PBI)2P] was synthesized and it was used to fabricate a hybrid system by aggregating with blue‐emitting, atomically precise copper nano‐clusters (Cu NCs). The interactions between these components [(PBI)2P] and Cu NCs] was thoroughly investigated by UV‐Vis spectroscopy and field emission gun transmission electron microscopy (FEG‐TEM).The effect of interaction between components on the optical properties of the hybrids was investigated by fluorescence spectroscopy. Interestingly, this hybrid system shows fluorescence thermometric behaviour at the 20–50 °C temperature range with a promising reversible switchable fluorescence property. Photo‐switching property is enhanced significantly in the hybrid compared to that of the peptide tethered pelylene bisimides as it is evident from the improvement of photocurrent gain (Ion/Ioff) from 2.6 in [(PBI)2P] to 6.9 in the hybrid. Modulation of photo‐switching property along with the thermo‐sensitive fluorescence property in this hybrid holds a future promise for fabrication of devices from the peptide based hybrids.
Dynamic Color‐Switching of Hydrogel Micropillar Array under Ethanol Vapor for Optical Encryption
Authors: Zhou Ming‐Xia , Jin Feng , Wang Jian‐Yu , Dong Xian‐Zi , Liu Jie , Zheng Mei‐Ling 
Published: July 2023

AbstractResponsive structural colors from artificially engineered micro/nanostructures are critical to the development of anti‐counterfeiting, optical encryption, and intelligent display. Herein, the responsive structural color of hydrogel micropillar array is demonstrated under the external stimulus of ethanol vapor. Micropillar arrays with full color are fabricated via femtosecond laser direct writing by controlling the height and diameter of the micropillars according to the FDTD simulation. Color‐switching of the micropillar arrays is achieved in <1 s due to the formation of liquid film among micropillars. More importantly, the structural color blueshift of the micropillar arrays is sensitive to the micropillar diameter, instead of the micropillar height. The micropillar array with a diameter of 772 nm takes 400 ms to complete blueshift under ethanol vapor, while that with a diameter of 522 nm blueshifts at 2400 ms. Microscale patterns are realized by employing the size‐dependent color‐switching of designed micropillar arrays under ethanol vapor. Moreover, Morse code and directional blueshift of structural colors are realized in the micropillar arrays. The advantages of controllable color‐switching of the hydrogel micropillar array would be prospective in the areas of optical encryption, dynamic display, and anti‐counterfeiting.