bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quercetin Application for Common Carp (Cyprinus carpio): I. Effects on Growth Performance, Humoral Immunity, Antioxidant Status, Immune-Related Genes, and Resistance against Heat Stress
Authors: Armobin Kobra , Ahmadifar Ehsan , Adineh Hossein , Samani Mahsa Naderi , Kalhor Naser , Yilmaz Sevdan , Hoseinifar Seyed Hossein , Van Doan Hien 
Published: February 2023

This study was done to evaluate the effect of different quercetin levels on growth performance, immune responses, antioxidant status, serum biochemical factors, and high-temperature stress responses in common carp (Cyprinus carpio). A total number of 216 common carp with an average weight of

27.21
±
53

g were divided into 12 tanks (

four

treatments
×
three

replications

) and fed 0 mg/kg quercetin (T0), 200 mg/kg quercetin (T1), 400 mg/kg quercetin (T2), and 600 mg/kg quercetin (T3) for 60 days. There were significant differences in growth performance, and the highest final body weight (FBW), weight gain (WG), specific growth rate (SGR), and feed intake (FI) were observed in T2 and T3 (

P
<
0.05

). Different quercetin levels significantly increased complement pathway activity (ACH50) and lysozyme activity both before and after heat stress (

P
<
0.05

). Catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were significantly increased in fish exposed to heat stress, but fish fed with a supplemented diet with quercetin showed the lowest levels both before and after heat stress (

P
<
0.05

). Superoxide dismutase (SOD) levels were significantly enhanced in fish fed diets supplemented with quercetin in both phases (

P
<
0.05

). Different quercetin levels led to a significant decrease in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) before and after the challenging test (

P
<
0.05

). Glucose and cortisol levels were significantly higher in the control group compared to the other treatments in both phases (

P
<
0.05

). The expression of glutathione peroxidase (GPx) and lysozyme was markedly upregulated in fish fed with quercetin-supplemented diets (

P
<
0.05

). No marked effects were observed for growth hormone (GR) and interleukin-8 (IL8) (

P
>
0.05

). In conclusion, dietary quercetin supplementations (400-600 mg/kg quercetin) improved growth performance, immunity, and antioxidant status and increased tolerance to heat stress.
MEK Inhibition Synergizes with TYK2 Inhibitors in NF1-Associated Malignant Peripheral Nerve Sheath Tumors
Authors: Borcherding Dana C. , Amin Neha V. , He Kevin , Zhang Xiaochun , Lyu Yang , Dehner Carina , Bhatia Himanshi , Gothra Angad , Daud Layla , Ruminski Peter , Pratilas Christine A. , Pollard Kai , Sundby Taylor , Widemann Brigitte C. , Hirbe Angela C. 
Published: February 2023

AbstractPurpose:Malignant peripheral nerve sheath tumors (MPNST) are aggressive sarcomas with limited treatment options and poor survival rates. About half of MPNST cases are associated with the neurofibromatosis type 1 (NF1) cancer predisposition syndrome. Overexpression of TYK2 occurs in the majority of MPNST, implicating TYK2 as a therapeutic target.Experimental Design:The effects of pharmacologic TYK2 inhibition on MPNST cell proliferation and survival were examined using IncuCyte live cell assays in vitro, and downstream actions were analyzed using RNA-sequencing (RNA-seq), qPCR arrays, and validation of protein changes with the WES automated Western system. Inhibition of TYK2 alone and in combination with MEK inhibition was evaluated in vivo using both murine and human MPNST cell lines, as well as MPNST PDX.Results:Pharmacologic inhibition of TYK2 dose-dependently decreased proliferation and induced apoptosis over time. RNA-seq pathway analysis on TYK2 inhibitor–treated MPNST demonstrated decreased expression of cell cycle, mitotic, and glycolysis pathways. TYK2 inhibition resulted in upregulation of the MEK/ERK pathway gene expression, by both RNA-seq and qPCR array, as well as increased pERK1/2 levels by the WES Western system. The compensatory response was tested with dual treatment with TYK2 and MEK inhibitors, which synergistically decreased proliferation and increased apoptosis in vitro. Finally, combination therapy was shown to inhibit growth of MPNST in multiple in vivo models.Conclusions:These data provide the preclinical rationale for the development of a phase I clinical trial of deucravacitinib and mirdametinib in NF1-assosciated MPNST.
Daclatasvir, an Antiviral Drug, Downregulates Tribbles 2 Pseudokinase and Resensitizes Enzalutamide-Resistant Prostate Cancer Cells
Authors: Monga Jitender , Valeriote Frederick , Hwang Clara , Gadgeel Shirish , Ghosh Jagadananda 
Published: February 2023

AbstractFDA-approved enzalutamide is commonly prescribed to reduce the growth of advanced prostate cancer by blocking androgen receptor function. However, enzalutamide-resistant prostate cancer (ERPC) invariably develops and progresses to metastatic, lethal disease. Management of ERPC poses a special problem not only because available therapeutic regimens cannot effectively kill ERPC cells but also due to their propensity to invade large bones. Moreover, molecular mechanism(s) behind enzalutamide resistance is not properly understood, which is delaying development of newer agents. We found that the pseudokinase, Tribbles 2 (TRIB2), is overexpressed in ERPC cells and plays a critical role in their survival. Forced overexpression of TRIB2 enhances prostate cancer cell growth and confers resistance to physiologic doses of enzalutamide, suggesting that TRIB2 plays an important role in the development and progression of ERPC. Though TRIB2 has emerged as an excellent molecular target for ERPC, suitable inhibitors are not commercially available for effective targeting. By designing a luciferase-tagged TRIB2 fusion protein-based assay system, we screened a library of about 1,600 compounds and found that daclatasvir (DCV), an antiviral drug, effectively inhibits TRIB2-luciferase. We also found that DCV degrades TRIB2 proteins by direct binding and resensitizes ERPC cells to enzalutamide treatment. Moreover, DCV at lower, sublethal doses synergizes with enzalutamide to decrease the viability and induce apoptosis in prostate cancer cells. Because DCV is already approved by the FDA and well tolerated in humans, based on our findings, it appears that DCV is a promising new agent for development of an effective therapy for advanced, enzalutamide-resistant, lethal prostate cancer.
РОЗРОБКА МЕТОДИК ІДЕНТИФІКАЦІЇ ТА КІЛЬКІСНОГО ВИЗНАЧЕННЯ МАНГІФЕРИНУ В М’ЯКІЙ ЛІКАРСЬКІЙ ФОРМІ
Authors: Yaromiy M. V. , Polovko N. P. , Bevz N. Yu. 
Published: February 2023

Мета роботи – розробка методик ідентифікації та кількісного визначення АФІ в складі антигерпетичного гелю.
Матеріали і методи. Об´єктами дослідження є зразки гелю з мангіферином. Для ідентифікації мангефірину використовували метод ТШХ з ТШХ-пластинками із шаром силікагелю, флуоресцентним індикатором F254 та СЗ мангіферину. Кількісне визначення проводили методом абсорбційної спектрофотометрії. Використовували спектрофотометр “Evolution 60s” (Thermo Fisher Scientific, США), аналітичні ваги “AXIS” (Польща), мірний посуд класу A, реактиви згідно вимог ДФУ.
Результати й обговорення. Мангефірин ідентифікували методом ТШХ після вилучення із гелю 70% спиртом у порівнянні зі СЗ мангіферину з використанням рухомої фази – суміші розчинників н-бутанол : оцтова кислота : вода (80 : 20 : 10), детектували в УФ-світлі за довжини хвилі 254 нм. Встановлено, що послідовність зон розчину порівняння та випробуваного розчину співпадають. Кількісне визначення мангіферину проводили методом абсорбційної спектрофотометрії в 70% етанолі за довжини хвилі 373 нм. Доведено, що підпорядкування розчинів мангефірину за довжини хвилі 373 нм закону Бугера-Ламберта-Бера спостерігається в межах концентрації від 0,002 мг/мл до 0,020 мг/мл. Розрахована прогнозована повна невизначеність результатів методики становить 1,21% £ 1,60% свідчить про коректність проведення випробувань в іншій лабораторії. При вивченні валідаційних характеристик методики встановлено, що методика робасна (Δt ≤ δmax = 0,51 %, досліджуваний розчин і розчин порівняння стійкі протягом не менше 1 години), специфічна (d 0,18 % £ 0,51%); лінійна (r 0,9999 ≥ min r 0,9981); прецизійна (% = 0,48 % ≤ 1,60 %) і правильна (виконується критерій практичної незначущості систематичної похибки 0,03 %).
Висновки. Для ідентифікації мангіферину в гелі запропоновано з метод ТШХ. Для кількісного визначення мангіферину розроблено метод абсорбційної спектрофотометрії у середовищі 70% етанолу за довжини хвилі 373 нм. Доведено, що за такими валідаційними характеристиками, як лінійність, прецизійність, правильність, робасність і специфічність розроблена методика є коректною і може бути використана в аналізі мангіферину в гелі.
Russia's Technology Imports from East  Asia
Authors: Simola Heli , Röyskö Aino 
Published: February 2023

AbstractRussia's invasion in Ukraine has led to substantial restrictions on exports to Russia, which some East Asian economies have joined. We review the importance of East Asian economies as providers of technology imports for Russia and present a preliminary analysis on the development of Russian imports from East Asia after the invasion. We find that East Asian economies have been important suppliers of technology goods for Russia. After February 2022, exports of most East Asian countries to Russia have dropped substantially. Chinese exports to Russia have increased, especially in the category of vehicles.
Diversifying Boundary Organizations: The Making of a Global Platform for Indigenous (and Local) Knowledge in the UNFCCC
Authors: López-Rivera Andrés 
Published: February 2023

Abstract
The creation of the Local Communities and Indigenous Peoples Platform under the United Nations Framework Convention on Climate Change (UNFCCC) set out to incorporate Indigenous and local knowledge into the science–policy landscape of the climate field. The Platform is a crucial case of institutional change, as it signals an incipient shift from a science-centric toward a pluralistic approach to knowledge in global climate governance. This article traces this process of change in the politics and practices underlying the establishment and design of the Platform as an interface for Indigenous and local knowledge holders. The analysis shows that the sui generis design of the Platform was the product of bricolage (recombination) and translation (recontextualization) of disparate elements with the purpose of accommodating various political demands in an altogether new kind of knowledge–policy interface: a diverse boundary organization. The article makes an empirical contribution to the historical development of knowledge politics in the UNFCCC and a theoretical contribution to the study of boundary organizations by advancing a broader conceptualization that transcends science-centric approaches.
Can Time to Treatment Reduce In-Hospital Mortality and Morbidity Among Surgical and Nonsurgical Elderly Patients With Sepsis
Authors: Wasfie Tarik , Buzadzhi Anna , Naisan Mursal , Senger Barbara , Memar Shayan , Tangalos Alexandra , Hella Jennifer , Hood Shannon , Barber Kimberly 
Published: February 2023

Sepsis mortality remains high and efforts to reduce it are continuing. We collected data from our patients presented to the emergency department (ED) with sepsis and performed a retrospective analysis of 1079 patients seen in the ED with sepsis during 2018 and 2020, before and after implementation of the new CDC protocol. Statistical analysis was performed using Student’s t-test and chi square test as well as Cox regression analysis. The patients were divided into pre-protocol (group 1) and post-protocol (group 2). A total of 1079 patients were included in the study. The mean age was 65 + 16.86 years, divided equally between gender (male 49%, female 51%). Patients with certain comorbidities showed statistically significant survival rate in the protocol group. The current protocol for sepsis when implemented will improve patients’ survival, in both surgical and medical patients and significantly in those with comorbid conditions.
Sluggish Cognitive Tempo Self Report Scale (SCT-SR): Development and Initial Validation Study
Authors: Gozpinar Nuran , Cakiroglu Suleyman , Gormez Vahdet 
Published: February 2023

Objective: Sluggish Cognitive Tempo (SCT) is a psychological construct consisting of daydreaming, confusion, slowness in movements, lethargy, and low motivation. It is important to develop a self-report scale to evaluate SCT symptoms in children and adolescents in order to meet the following needs; (1) Considering the closeness of SCT symptoms with internalized symptoms, it can provide more reliable information, (2) It allows information to be obtained from multiple informants, and (3) The main symptoms of SCT include subjective experiences such as daydreaming and mind wandering that may be difficult to observe from the outside. Therefore, it was aimed to develop a self-report SCT scale. Method: A total of 467 adolescents and 178 parents participated in the study. Data were collected with scales. Results: The scale consists of 20 items and a single factor. Factor loadings were between 0.60 and 0.81. Conclusion: Sluggish Cognitive Tempo Self-Report Scale is a valid and reliable self-report scale.
Validation of Prognostic Club Cell Secretory Protein (CC16) Cut-point in an Independent ALTA Cohort
Authors: Almuntashiri Sultan , Chase Aaron , Sikora Andrea , Zhang Duo 
Published: February 2023

Background: Club cell secretory protein (CC16) has demonstrated utility as a lung-specific biomarker in predicting mortality in acute respiratory distress syndrome (ARDS). These findings have been observed in pre-clinical trials and a re-analysis of a large, randomized controlled trial of ARDS (Fluid and Catheter Treatment Trial (FACTT)). Objectives: The purpose of this study was to validate previous findings by evaluating CC16 level as a mortality predictor in patients from the albuterol to treat acute lung injury (ALTA) trial. Design and Method: In this secondary biomarker analysis, plasma CC16 level was measured from 100 ALTA subjects using enzyme-linked immunosorbent assay (ELISA). The rate of mortality was assessed in patients with high (⩾45 ng/mL) versus low CC16 (<45 ng/mL) levels. This cut-off level was applied based on our previous analysis from FACTT trial. Significance was assessed using Kaplan-Meier curves and a log-rank test. Results: Subjects were an average of 50 years old and 46% of them were females. Patients with high CC16 levels had higher 90-day mortality compared to those with low CC16 levels, (37.73% vs 8.95%, P < .001). Other clinical outcomes including ICU-free days, ventilator-free days, and organ failure free days were significantly different between the groups (All P < .05). Conclusion: In this validation study, we demonstrated that ARDS patients with high plasma CC16 concentration had a higher mortality rate than those with low CC16 levels, confirming previous findings that CC16 levels are associated with ARDS mortality.
Kienböck’s disease: preventing disease progression in early-stage disease
Authors: Salva-Coll Guillem , Esplugas Mireia , Carreño Ana , Lluch-Bergada Alex 
Published: February 2023

Currently Kienböck’s disease remains an ‘unsolved’ problem in hand surgery. Different factors have been associated with the avascular necrosis of the lunate. Mechanical, vascular and biological factors, alone or in combination, may have an influence in the aetiopathogenesis and determine the progress of the disease and even the results of the treatment. This is especially relevant in the early stages, in which conservative or surgical treatment may modify the natural history of the disease, maintaining the lunate structure and thus preserving the joint surfaces. There are multiple surgical treatments for Kienböck’s disease in the early stages, before lunate collapse; each one is based on one of the possible factors that can cause avascular necrosis of the lunate. The objective is not only to treat symptoms but to prevent progression. This article is a review of the most frequent treatments used in the early stages and a personal view of the authors. Level of evidence: V
Pathogen detection in RNA-seq data with Pathonoia
Authors: Liebhoff Anna-Maria , Menden Kevin , Laschtowitz Alena , Franke Andre , Schramm Christoph , Bonn Stefan 
Published: February 2023

Abstract
Background
Bacterial and viral infections may cause or exacerbate various human diseases and to detect microbes in tissue, one method of choice is RNA sequencing. The detection of specific microbes using RNA sequencing offers good sensitivity and specificity, but untargeted approaches suffer from high false positive rates and a lack of sensitivity for lowly abundant organisms.

Results
We introduce Pathonoia, an algorithm that detects viruses and bacteria in RNA sequencing data with high precision and recall. Pathonoia first applies an established k-mer based method for species identification and then aggregates this evidence over all reads in a sample. In addition, we provide an easy-to-use analysis framework that highlights potential microbe-host interactions by correlating the microbial to the host gene expression. Pathonoia outperforms state-of-the-art methods in microbial detection specificity, both on in silico and real datasets.

Conclusion
Two case studies in human liver and brain show how Pathonoia can support novel hypotheses on microbial infection exacerbating disease. The Python package for Pathonoia sample analysis and a guided analysis Jupyter notebook for bulk RNAseq datasets are available on GitHub.
Effect of surgical pleth index-guided remifentanil administration on perioperative outcomes in elderly patients: a prospective randomized controlled trial
Authors: Won Young Ju , Oh Seok Kyeong , Lim Byung Gun , Kim Young Sung , Lee Do Yeop , Lee Jae Hak 
Published: February 2023

Abstract
Background
During general anesthesia, the surgical pleth index (SPI) monitors nociception. The evidence of SPI in the elderly remains scarce. We aimed to investigate whether there is a difference in perioperative outcomes following intraoperative opioid administration according to the surgical pleth index (SPI) value versus hemodynamic parameters (heart rate or blood pressure) in elderly patients.

Methods
Patients aged 65–90 years who underwent laparoscopic colorectal cancer surgery under sevoflurane/remifentanil anesthesia were randomized to receive remifentanil guided by SPI (SPI group) or conventional clinical judgment based on hemodynamic parameters (conventional group). The primary endpoint was intraoperative remifentanil consumption. Secondary endpoints were intraoperative hemodynamic instability, pain score, fentanyl consumption and delirium in the post-anesthesia care unit (PACU), and perioperative changes in interleukin-6 and natural killer (NK) cell activity.

Results
Seventy-five patients (38, SPI; 37, conventional) were included in the study. The SPI group consumed significantly more remifentanil intraoperatively than the conventional group (mean ± SD, 0.13 ± 0.05 vs. 0.06 ± 0.04 μg/kg/min, P < 0.001). Intraoperative hypertension and tachycardia were more common in the conventional group than in the SPI group. Pain score in the PACU (P = 0.013) and the incidence of delirium in the PACU were significantly lower in the SPI group than the conventional group (5.2% vs. 24.3%, P = 0.02). There was no significant difference in NK cell activity and interleukin-6 level.

Conclusions
In the elderly patients, SPI-guided analgesia provided appropriate analgesia with sufficient intraoperative remifentanil consumption, lower incidence of hypertension/ tachycardia events, and a lower incidence of delirium in the PACU than the conventional analgesia. However, SPI-guided analgesia may not prevent perioperative immune system deterioration.

Trial registration
The randomized controlled trial was retrospectively registered in the UMIN Clinical Trials Registry (trial number: UMIN000048351; date of registration: 12/07/2022).
A food bank program to help food pantries improve healthy food choices: mixed methods evaluation of The Greater Boston Food Bank’s Healthy Pantry Program
Authors: Jia Jenny , Burgun Rachel , Reilly Alexa , Sonnenblick Ross , Fiechtner Lauren , Zack Rachel M. , Porneala Bianca , Thorndike Anne N. 
Published: February 2023

Abstract
Background
The Greater Boston Food Bank’s (GBFB) Healthy Pantry Program (HPP) is an online training that teaches food pantry staff to implement behavioral nudges (e.g., traffic-light nutrition labels, choice architecture) to promote healthier client choices. This study assessed if HPP was associated with healthier food bank orders by food pantries and identified implementation facilitators and barriers.

Methods
This mixed methods study collected quantitative data from a matched cohort of 10 HPP food pantries and 99 matched control food pantries in eastern Massachusetts that allow clients to choose their own food, and qualitative data from structured individual interviews with 8 HPP pantry staff. A difference-in-differences analysis compared changes in percentage of pantries’ food bank orders (by weight) of foods labeled green/yellow (healthier choices) and fresh produce from baseline to 6 and 10 months between HPP and control pantries. Interviews were coded for implementation facilitators and barriers.

Results
Before starting HPP, green-yellow ordering was 92.0% (SD 4.9) in control and 87.4% (SD 5.4) in HPP pantries. Participation in HPP was not associated with changes in green-yellow or fresh produce ordering at 6 or 10 months. HPP implementation facilitators included HPP training being accessible (sub-themes: customizable, motivating) and compatible with client-choice values. Barriers included resource limitations (sub-themes: staff shortage, limited space) and concerns about stigmatizing client food choices with use of labels for unhealthy foods.

Conclusions
An online program to help pantries promote healthier client choices was not associated with changes in how much healthy food pantries ordered from the food bank, suggesting it did not substantially change client choices. Implementation challenges and high baseline healthy ordering may have influenced HPP’s effectiveness.