bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Roll Decay for Surface-Ship Model Experiments With Uncertainty Estimates
Authors: Park Joel T. 
Published: July 2023

Abstract
Roll decay of David Taylor model basin (DTMB) Model 5720, a 23rd-scale free-running model of the research vessel (R/V) Melville, is evaluated with uncertainty estimates. Experimental roll-decay time series was accurately modeled as an exponentially decaying cosine function, which is the solution of a second-order ordinary differential equation for damping coefficient of less than one (N &lt; 1). The curve-fit provides damping coefficient (N), period (T), and offset. Roll period in calm water was dependent on Froude number (Fr) and initial roll angle (a). Roll decay data are from 76 runs for three nominal Froude numbers, Fr = 0, 0.15, and 0.22. The initial roll angle variation was 3 deg–25 deg. The natural roll period was 2.139 ± 0.041 s (±1.9%). The decay coefficient data were approximated by a plane in three dimensions with Fr and initial roll amplitudes (a) as the independent variables. Curve-fit results are compared to decay coefficient by log decrement and period from time between zero crossings. Examples demonstrate average values for a single roll decay event from log decrement are the same as values by the curve-fitting method within uncertainty estimates. The uncertainty estimate for the decay coefficient is significantly less by curve-fit method in comparison to log-decrement method. By log decrement, the relative uncertainty increases with decreasing roll amplitude peak; consequently, focus should be on the damping coefficient at the largest peaks, where the uncertainty is the smallest.
Influence of Surface Geometry on Palm and Fist Contact Pressure Distribution During Strikes With the Hand in Automotive Assembly
Authors: Hausmanninger Lukas , Komnik Igor , Fleiter Mario , Potthast Wolfgang 
Published: July 2023

The increase in repetitive strain injuries to the hand underscores the need for assessing and preventing musculoskeletal overuse associated with hand-intensive tasks. This study investigates the risk of overload injuries in soft tissue structures of the hand by analyzing the pressure distribution and location of peak pressure in the hand during snap-fit connection assembly in the automotive industry. The influence of the surface geometry of automotive trim components the pressure distribution and force imparted during strikes with the palm and the fist are investigated in a cohort of 30 subjects with extensive experience installing trim parts with snap-fit connections. Using the palm or fist (ulnar hand side) of the dominant hand, the subjects struck a simulation device with a flat, rounded, or edged surface geometry. The average peak force applied was 600 N (±122 N), nearly 3 times the force required to overcome the technical resistance of the snap-fit connector (220 N). Fist strikes exerted a 40% higher mean peak pressure and 18% higher mean pressure than did palm strikes. The pressure distribution in the region of the thenar eminence and soft tissue of the ulnar side of the hand did not differ between fist strikes on flat and edged surfaces. Considering the delicate anatomy of the hand, especially the hypothenar muscles on the ulnar side, assembling connection claps using the fist instead of the palm may prevent repetitive blunt trauma to the sensitive blood vessels and nerves in the palm.
Bacterial secretion systems contribute to rapid tissue decay in button mushroom soft rot disease
Authors: Wein Philipp , Dornblut Katharina , Herkersdorf Sebastian , Krüger Thomas , Molloy Evelyn M. , Brakhage Axel A. , Hoffmeister Dirk , Hertweck Christian 
Published: July 2023

ABSTRACT

The soft rot pathogen
Janthinobacterium agaricidamnosum
causes devastating damage to button mushrooms (
Agaricus bisporus
), one of the most cultivated and commercially relevant mushrooms. We previously discovered that this pathogen releases the membrane-disrupting lipopeptide jagaricin. This bacterial toxin, however, could not solely explain the rapid decay of mushroom fruiting bodies, indicating that
J. agaricidamnosum
implements a more sophisticated infection strategy. In this study, we show that secretion systems play a crucial role in soft rot disease. By mining the genome of
J. agaricidamnosum
, we identified gene clusters encoding a type I (T1SS), a type II (T2SS), a type III (T3SS), and two type VI secretion systems (T6SSs). We targeted the T2SS and T3SS for gene inactivation studies, and subsequent bioassays implicated both in soft rot disease. Furthermore, through a combination of comparative secretome analysis and activity-guided fractionation, we identified a number of secreted lytic enzymes responsible for mushroom damage. Our findings regarding the contribution of secretion systems to the disease process expand the current knowledge of bacterial soft rot pathogens and represent a significant stride toward identifying targets for their disarmament with secretion system inhibitors.

IMPORTANCE

The button mushroom (
Agaricus bisporus
) is the most popular edible mushroom in the Western world. However, mushroom crops can fall victim to serious bacterial diseases that are a major threat to the mushroom industry, among them being soft rot disease caused by
Janthinobacterium agaricidamnosum
. Here, we show that the rapid dissolution of mushroom fruiting bodies after bacterial invasion is due to degradative enzymes and putative effector proteins secreted via the type II secretion system (T2SS) and the type III secretion system (T3SS), respectively. The ability to degrade mushroom tissue is significantly attenuated in secretion-deficient mutants, which establishes that secretion systems are key factors in mushroom soft rot disease. This insight is of both ecological and agricultural relevance by shedding light on the disease processes behind a pathogenic bacterial-fungal interaction which, in turn, serves as a starting point for the development of secretion system inhibitors to control disease progression.
A combined microscopy and single-cell sequencing approach reveals the ecology, morphology, and phylogeny of uncultured lineages of zoosporic fungi
Authors: Seto Kensuke , Simmons D. Rabern , Quandt C. Alisha , Frenken Thijs , Dirks Alden C. , Clemons Rebecca A. , McKindles Katelyn M. , McKay R. Michael L. , James Timothy Y. 
Published: July 2023

ABSTRACT

Environmental DNA analyses of fungal communities typically reveal a much larger diversity than can be ascribed to known species. Much of this hidden diversity lies within undescribed fungal lineages, especially the early diverging fungi (EDF). Although these EDF often represent new lineages even at the phylum level, they have never been cultured, making their morphology and ecology uncertain. One of the methods to characterize these uncultured fungi is a single-cell DNA sequencing approach. In this study, we established a large data set of single-cell sequences of EDF by manually isolating and photographing parasitic fungi on various hosts such as algae, protists, and micro-invertebrates, combined with subsequent long-read sequencing of the ribosomal DNA locus (rDNA). We successfully obtained rDNA sequences of 127 parasitic fungal cells, which clustered into 71 phylogenetic lineages belonging to seven phylum-level clades of EDF: Blastocladiomycota, Chytridiomycota, Aphelidiomycota, Rozellomycota, and three unknown phylum-level clades. Most of our single cells yielded novel sequences distinguished from both described taxa and existing metabarcoding data, indicating an expansive and hidden diversity of parasitic taxa of EDF. We also revealed an unexpected diversity of endobiotic
Olpidium
-like chytrids and hyper-parasitic lineages. Overall, by combining photographs of parasitic fungi with phylogenetic analyses, we were able to better understand the ecological function and morphology of many of the branches on the fungal tree of life known only from DNA sequences.

IMPORTANCE
Much of the diversity of microbes from natural habitats, such as soil and freshwater, comprise species and lineages that have never been isolated into pure culture. In part, this stems from a bias of culturing in favor of saprotrophic microbes over the myriad symbiotic ones that include parasitic and mutualistic relationships with other taxa. In the present study, we aimed to shed light on the ecological function and morphology of the many undescribed lineages of aquatic fungi by individually isolating and sequencing molecular barcodes from 127 cells of host-associated fungi using single-cell sequencing. By adding these sequences and their photographs into the fungal tree, we were able to understand the morphology of reproductive and vegetative structures of these novel fungi and to provide a hypothesized ecological function for them. These individual host-fungal cells revealed themselves to be complex environments despite their small size; numerous samples were hyper-parasitized with other zoosporic fungal lineages such as Rozellomycota.
Whole-genome sequencing and annotation of selected
Enterobacteriaceae
derived from commercial cucumber fermentation
Authors: Page Clinton A. , Sattar Arwa A. , Pérez-Díaz Ilenys M. 
Published: July 2023

ABSTRACT

We report five whole-genome sequences, along with annotations, representing
Enterobacteriaceae
from the genera
Enterobacter
(
n
= 1),
Pantoea
(
n
= 1), and
Leclercia
(
n
= 3) isolated from commercial cucumber fermentations performed in North Carolina and Minnesota, USA.
Caring for the carers: an evaluation of the recovery, readjustment and reintegration programme (R3P)
Authors: Simms Amos , Leightley D , Lamb D 
Published: July 2023

Introduction
The challenges faced by healthcare workers, not least during the response to the COVID-19 pandemic, have been extensively studied, and concerns continue to be highlighted in relation to their long-term mental health. Identifying the need to support their personnel, a leader-led structured programme of reflection: the recovery, readjustment and reintegration programme (R3P) was designed by the UK Defence Medical Services to mitigate the potential stressors associated with this outbreak and enhance the resilience of the workforce.

Methods
128 military personnel completed an evaluation of R3P. A survey included measures of anxiety before and after the intervention, perceptions of the discussion themes and whether these brought a sense of closure to areas of distress, and attitudes to help-seeking.

Results
Most respondents (86%�92%) rated the five discussion themes either �helpful� or �very helpful�, 51% of respondents reported a sense of closure about an issue that had been causing distress and 72% of respondents felt better able to seek help should it be necessary. Evaluating the effect R3P had on anxiety, a Wilcoxon signed rank test elicited a statistically significant difference in anxiety pre-R3P and post-R3P; Z=-3.54, p<0.001. The median anxiety rating was 3.5 (IQR 4.75, 95% CI 1.25 to 6.00) before undertaking R3P, which decreased to 3 (IQR 4.75, 95% CI 1.00 to 5.75) after undertaking R3P. 39.1% of participants reported decreased anxiety, 18.8% reported increased anxiety and 42.2% reported no change.

Conclusion
This evaluation has identified several positive aspects to R3P with many personnel reporting a reduction in anxiety, a sense of closure and increased likelihood of help-seeking. Several participants did report an increase in anxiety and the long-term impact of R3P on mental health and well-being is unclear. Further mixed-methods evaluation incorporating a longer follow-up is required.
Ground state degeneration of frustrated antiferromagnetic Ising wheels
Authors: Florek Wojciech , Jaworski Jerzy 
Published: July 2023

In this paper, a finite spin system corresponding to magnetic molecules with the wheel geometry is discussed in the Ising-like limit of the Heisenberg Hamiltonian. We put stress on the ground state degeneration, which is determined with the use of some discrete mathematics methods, especially investigating some integer sequences, including the famous Fibonacci one.
Preparation and Characterization of Patuletin-Loaded Chitosan Nanoparticles with Improved Selectivity and Safety Profiles for Anticancer Applications
Authors: Metwaly Ahmed M. , Abdel-Raoof Ahmed M. , Alattar Abdulaziz M. , El-Zomrawy Adham A. , Ashmawy Ashraf M. , Metwally Metwally G. , Abu-Saied Mohamed A. , Lotfy Asmaa M. , Alsfouk Bshra A. , Elkaeed Eslam B. , Eissa Ibrahim H. 
Published: July 2023

Objective. This study aimed to investigate the preparation of patuletin-encapsulated chitosan nanoparticles (PT-CS-NPs) using the ionic gelation method, evaluate their potential as anticancer agents, and invent a new method of differential pulse voltammetric analysis for patuletin (PT). Methods. Computational studies were conducted to assess the affinity of PT for chitosan, confirming a promising interaction. PT-CS-NPs were synthesized based on the computational outputs, resulting in nanoparticles with an angular structure and an average size of 6.168 nm. The cytotoxicity of PT and PT-CS-NP was evaluated in breast and colon cancer cell lines, and selectivity indices were calculated to assess safety profiles. The electrochemical behavior of PT was also investigated using the Britton–Robinson buffer. Results. PT-CS-NP exhibited both potent cytotoxicity and a favorable safety profile, showing the most active and safest pattern of cytotoxicity among the tested compounds. The electrochemical oxidation of PT was observed at 0.531 V vs. Ag/AgCl at pH 4.0.0. The concentration of PT showed a linear relationship with the corresponding peak current over the range of

10.0

x

10

−
9

:

10.0

x

10

−
5

M

M, with a minimum limit of detection of 3.4 × 10(−9)M. The proposed method successfully measured PT, with a relative standard deviation below 2%. Conclusion. The preparation of PT-CS-NP via the ionic gelation method resulted in angular nanoparticles with a promising anticancer activity and safety profiles. The electrochemical behavior of PT was characterized, and a reliable method for PT quantification was established.
Time-Dependent Prognostic Value of Serological and Measurable Residual Disease Assessments after Idecabtagene Vicleucel
Authors: Paiva Bruno , Manrique Irene , Rytlewski Julie , Campbell Timothy , Kazanecki Christian C. , Martin Nathan , Anderson Larry D. , Berdeja Jesús G. , Lonial Sagar , Raje Noopur S. , Lin Yi , Moreau Philippe , San-Miguel Jesús F. , Munshi Nikhil C. , Kaiser Shari M. 
Published: July 2023

Abstract

The role of measurable residual disease (MRD) in multiple myeloma patients treated with chimeric antigen receptor (CAR) T cells is uncertain. We analyzed MRD kinetics during the first year after idecabtagene vicleucel (ide-cel) infusion in 125 relapsed/refractory multiple myeloma patients enrolled in KarMMa. At month 1 after ide-cel, there were no differences in progression-free survival (PFS) between patients in less than complete response (CR) versus those in CR; only MRD status was predictive of significantly different PFS at this landmark. In patients with undetectable MRD at 3 months and beyond, PFS was longer in those achieving CR versus &lt;CR. Persistent MRD in the 10−6 logarithmic range and reappearance of normal plasma cells in MRD-negative patients were associated with inferior PFS. This study unveils different prognostic implications of serological and MRD response dynamics after ide-cel and suggests the potential value of studying the reappearance of normal plasma cells as a surrogate of loss of CAR T-cell functionality.

Significance:
This is one of the first studies evaluating the impact of CR and MRD dynamics after CAR T therapy in relapsed/refractory multiple myeloma. These data help interpret the prognostic significance of serological and MRD responses at early and late time points after CAR T-cell infusion.
See related commentary by Landgren and Kazandjian, p. 346 .
This article is featured in Selected Articles from This Issue, p. 337
Have we made progress in taking care of adolescents and young adults with cancer? Results of a European multi-professional survey
Authors: Bozovic Spasojevic Ivana , Ferrari Andrea , De Munter Johan , Gamble Ashley , Konsoulova-Kirova Assia , Rizvi Katie , Schneider Carina , Balsat Marie , Castleton Anna , Gofti-Laroche Leila , Kienesberger Anita , Timmermann Beate , Vormoor Josef , Saloustros Emmanouil , Stark Daniel P 
Published: July 2023

Background: It is well documented that traditional health care models do not meet the specific needs of Adolescents and Young Adults (AYA) cancer patients. Methods: We explore a map of the development of age-specific AYA cancer care across Europe, from the perspective of healthcare professionals with an interest in AYA care, in order to understand the specific challenges and map progress over time. An on-line survey was developed by international professional cancer organisations. Results: We had 377 respondents from 60 countries. The majority of respondents were physicians 298 (79%), a minority of survey respondents (39, 10.4%) work exclusively with AYA patients, most respondents declared substantial and routine clinical service collaborations to provide care and treatment to AYA with cancer. Policy for the multidisciplinary management of AYA cancer patients commonly appears in Europe now, and was reported by 234 (78.52%) respondents. Specific professional training for AYA cancer care is not uniformly available. Conclusion: There is considerable opportunity for many organisations to work together in raising the profile of AYA cancer related issues, in providing education and in encouraging research and collaboration.
Republication of “Most Readmissions Following Ankle Fracture Surgery Are Unrelated to Surgical Site Issues: An Analysis of 5056 Cases”
Authors: Fu Michael C. , Young Kelsey , Cody Elizabeth , Schairer William W. , Demetracopoulos Constantine A. , Ellis Scott J. 
Published: July 2023

Background: Ankle fracture surgeries are generally safe and effective procedures; however, as quality-based reimbursement models are increasingly affected by postoperative readmission, we aimed to determine the causes and risk factors for readmission following ankle fracture surgery. Methods: Ankle fracture cases were identified from the prospectively collected American College of Surgeons National Surgical Quality Improvement Program from 2013 to 2014. Demographics, comorbidities, and fracture characteristics were collected. Rates of 30-day adverse events and readmissions were determined as well as the causes for readmission. Multivariable logistic regression analyses were performed to identify risk factors associated with having any adverse events and being readmitted within 30 days of surgery. Results: There were 5056 patients included; 167 (3.3%) were open fractures. The rate of any postoperative adverse event was 5.2%. There were 116 unplanned readmissions, with a readmission rate of 2.3%. Of the 116 unplanned readmissions, 49 (42.2%) were for reasons related to the surgery or surgical site, with the most common causes being deep surgical site/hardware infections (12.9%), superficial site infections (11.2%), and wound disruption (6.9%). Most readmissions were for reasons unrelated to the surgical site (51.7%), including cardiac disorders (8.6%), pulmonary disorders (7.8%), and neurological/psychiatric disorders (6.9%). The cause of readmission was unknown for 6% of readmissions. With multivariable logistic regression, the strongest risk factors for readmission were a history of pulmonary disease (odds ratio [OR], 2.29), American Society of Anesthesiologists (ASA) class ≥3 (OR, 2.28), and open fractures (OR, 2.04) (all P < .05). Conclusion: In this cohort of 5056 ankle fracture cases, 2.3% of patients were readmitted within 30 days, with at least 51.7% of all unplanned readmissions due to causes unrelated to the surgery or surgical site. Predictors of readmission included a history of pulmonary disease, higher ASA class, and open fractures. Based on these findings, we advocate close medical follow-up with nonorthopaedic providers after discharge for high-risk patients. Level of Evidence: Level III.
The longitudinal outcomes of mental health for Flemish care leavers
Authors: Gypen Laura , Stas Lara , West Delphine , Van Holen Frank , Vanderfaeillie Johan 
Published: July 2023

Young people leaving foster- and residential care more often indicate having mental health problems than their peers from the general population. International research has extensively studied these mental health outcomes of care leavers. However, in Flanders (the Dutch speaking part of Belgium), no previous quantitative studies are known. This longitudinal study explores outcomes of depression, anxiety, sleep and general mental health of care leavers in Flanders and investigates if these outcomes improve in the years after leaving care. Associations with placement factors are explored to support recommendations to the clinical field. The current study involves 175 care-leavers. The participants of the study are between 18 and 27 years old at the start of the study and have all left care. Data was collected using a self-reporting questionnaire touching multiple domains. On all domains, no significant differences between foster- and residential care leavers are found. Residential care leavers more often indicate depressive symptoms than their peers from the general population. Foster care leavers more often indicate sleeping problems. Regarding anxiety, no differences are found. In all conducted analyses, the number of years in care, the age of starting and leaving care, the different types of support during care and whether aftercare was offered were included. However, none of these factors provide a sufficient explanation for the outcomes found. On all scales, the effect of gender, the number of placements and the reason of placement do recur. This indicates the importance of these factors. Policy makers should focus on improving placement stability and extra support for children who were placed due to own emotional or behavioral problems or maltreatment.
Rewitzerite, K(H2O)Mn2(Al2Ti)(PO4)4[O(OH)](H2O)10⋅4H2O, a new monoclinic paulkerrite-group mineral, from the Hagendorf-Süd pegmatite, Oberpfalz, Bavaria, Germany
Authors: Grey Ian E. , Hochleitner Rupert , Kampf Anthony R. , Boer Stephanie , MacRae Colin M. , Mumme William G. , Keck Erich 
Published: July 2023

AbstractRewitzerite, K(H2O)Mn2(Al2Ti)(PO4)4[O(OH)](H2O)10⋅4H2O, is a new monoclinic member of the paulkerrite group, from the Hagendorf-Süd pegmatite, Oberpfalz, Bavaria, Germany. It was found in specimens of altered zwieselite, in association with rockbridgeite. Rewitzerite forms clusters of colourless elongated hexagonal-shaped prisms, up to 0.1 mm long. The crystals are flattened on {010} and elongated along [100], with forms {010}, {001}, {111} and {$\bar{1}$11}. The calculated density is 2.33 g⋅cm–3. Optically, rewitzerite crystals are biaxial (+), with α = 1.585(2), β = 1.586(2), γ = 1.615(2) (measured in white light) and 2V(meas) = 25(2)°. The empirical formula from electron microprobe analyses and structure refinement is A1[K0.77(H2O)0.23]A2[H2O] M1(Mn2+0.82Mg0.64Fe3+0.43□0.11)Σ2.00M2+M3(Al1.51Ti4+1.06Fe3+0.43)Σ3.00(PO4)4X[(OH)0.54F0.42O1.04]Σ2.00(H2O)10⋅4H2O, where □ = vacancy.Rewitzerite has monoclinic symmetry with space group P21/c and unit-cell parameters a = 10.444(2) Å, b = 20.445(2) Å, c = 12.2690(10)Å, β = 90.17(3)°, V = 2619.8(6) Å3 and Z = 4. The crystal structure was refined using synchrotron single-crystal data to wRobs = 0.068 for 5894 reflections with I > 3σ(I). The crystal structure has the same topology as that for orthorhombic paulkerrite-group minerals but differs primarily in having an ordering of K+ and H2O molecules in different A sites, whereas they are disordered at a single A site in the orthorhombic members of the group.