bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social prescribing for adults with chronic pain in the U.K.: a rapid review
Authors: Pilkington Gerlinde , Johnson Mark I. , Thompson Kate 
Published: January 2025

Introduction Social prescribing links patients to community groups and services to meet health needs; however, it is uncertain what the benefits and impacts of social prescribing are for people with chronic pain. The National Institute for Health and Care Excellence (NICE) undertook a systematic review to investigate the clinical and cost effectiveness of social interventions aimed at improving the quality of life of people with chronic pain; no relevant clinical studies comparing social interventions with standard care for chronic pain were found, though the inclusion criteria for studies was narrow. Objectives To undertake a rapid review of all types of research and policy on social prescribing for adults with chronic pain in the U.K. (i) to describe the characteristics of relevant research and (ii) to synthesise data on impact. Methods A two-stage rapid review was planned. Stage (i) scoped and categorised knowledge from a comprehensive representation of the literature. In stage (ii), we undertook a descriptive synthesis of quantitative data along with a thematic analysis of qualitative data identified by stage (i). Results Of 40 full-text records assessed for inclusion, three met the inclusion criteria from academic databases. An additional five records were found in grey literature. Six records reported quantitative findings suggesting that social prescribing reduced pain severity and discomfort, pain medication and clinical appointments; and improved quality of life and ability to manage health. Five records captured qualitative data from interviews, case studies and anecdotal quotes that suggested positive impact on health and wellbeing; and increased self-efficacy in social prescribers undertaking training on pain. Conclusions There is tentative evidence that social prescribing improves health and wellbeing outcomes in adults with chronic pain and that there is a need to upskill social prescribers in contemporary pain science education. Research on the routes to referral, outcomes and impacts is needed. Perspective Social prescribing is valued and may be of benefit for people with chronic pain. There is a need to further develop and evaluate social prescribing services for people with chronic pain to enhance holistic patient centered care.
Prioritizing Patients from the Most Deprived Areas on Elective Waiting Lists in the NHS in England: Estimating the Health and Health Inequality Impact
Authors: Gibbs Naomi Kate , Griffin Susan , Gutacker Nils , Villaseñor Adrián , Walker Simon 
Published: January 2025

Introduction. Reducing hospital waiting lists for elective procedures is a policy concern in the National Health Service (NHS) in England. Following growth in waiting lists after COVID-19, the NHS published an elective recovery plan that includes an aim to prioritize patients from deprived areas. We use a previously developed model to estimate the health and health inequality impact under hypothetical targeted versus universal policies to reduce waiting time. Methods. We use a Markov model to estimate the health impact of waiting, by index of multiple deprivation quintile group, for 8 elective procedures. We estimate patients’ remaining quality-adjusted life-years (QALYs) with baseline waiting times and under 2 hypothetical policy scenarios: 1) a universal policy in which all patients receive an equal reduction in wait and 2) a targeted policy in which patients living in the most deprived quintile are prioritized. We estimate individual and population level health under each of the 2 policies and compare it with baseline. We also estimate how health inequality changes from baseline using the slope index of inequality, reflecting the difference in health between the least and most deprived quintile based on QALYs. Results. A universal reduction in waiting time is estimated to improve overall population health but increase health inequality. A targeted reduction would achieve nearly the same overall health gain and would also increase population-level health inequalities but to a lesser extent than the universal policy would. Discussion. If the NHS is successful in prioritizing patients on waiting lists from the most deprived areas, this may result in smaller increases in health inequalities while maintaining a similar level of overall health gain compared with a universal policy. Highlights The NHS elective recovery plans include prioritizing patients who live in the most deprived areas of England. Evaluating a hypothetical targeted wait time reduction policy against a universal wait time reduction policy suggests almost the same level of population health gain could be achieved while lessening the negative impact on health inequality. Expected outcomes of government health policies should be quantified to explore the impact on both health maximization and health inequality minimization, as both represent legitimate policy concerns.
Impact of single nucleotide polymorphisms (SNPs) in antioxidant-enzyme genes on the concentrations of folate, homocysteine and glutathione in plasma from healthy subjects after folic acid supplementation – a randomized controlled crossover trial
Authors: Mansoor Mohammad Azam , Stea Tonje Holte , Slettan Audun , Perera Erandie , Maddumage Ridmi , Kottahachchi Darshana , Ali Dhikra Saleem , Cabo Rona , Blomhoff Rune 
Published: January 2025

Abstract

Background
One-carbon metabolism links folate and methionine metabolism and this is essential for nucleotide synthesis in the cells. Alterations in one-carbon metabolism are associated with cardiovascular disease (CVD), type 2 diabetes and cancer. Our aim was to investigate whether SNPs in antioxidant-enzyme genes impact the concentrations of folate in serum (s-folate), plasma total homocysteine (p-tHcy) and total glutathione in plasma (p-tGSH) in healthy subjects after supplementation with folic acid.

Methods
In a randomized, double blind, crossover study, healthy subjects received 0.8 mg folic acid per day or a placebo for two weeks. Twenty-four male, and sixty-seven female subjects participated in this study. Participants were aged 36.4 ± 14.8 years (mean ± SD). We studied SNPs in six genes by PCR methods. The concentrations of s-folate, p-tHcy and p-tGSH were measured in fasting samples with Cobas and an HPLC-fluorescence method. Student T-tests and ANOVA were used for the statistical calculations.

Main findings
The subjects with SNP (rs4880) in superoxide dismutase (SOD2) gene (CC) allele had higher concentrations of s-folate and lower concentrations of p-tHcy than subjects with (CT + TT) alleles, (p = 0.014 and p = 0.012). Contrary to SOD2 (CC) allele, the subjects with SNP (rs1001179) catalase (CAT) CC allele had lower concentrations of s-folate (p = 0.029), higher concentrations of p-tGSH (0.017) and higher concentrations of p-tHcy before and after folic acid supplementations (p = 0.015, p = 0.017) than the subjects with (CT + TT) allele. Glutathione transferase (theta)1 (GST-T1) genotype was associated with higher concentrations of s-folate than GST-T0 before (p = 0.025) and after folic acid supplementation (p = 0.047). SNP (rs1050450) in glutathione peroxidase (GPX1) had also impact on the concentrations of p-tGSH (p = 0.011) in healthy subjects.

Conclusion
SNPs in SOD2 (rs4880), CAT (rs1001179), and GST1 impact the concentrations of s-folate, and p-tHcy in healthy subjects before and after folic acid supplementation. Our findings suggest that SNPs in antioxidant-genes have a role in health and disease by impacting the concentrations of s-folate, p-tHcy and p-tGSH.
Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell lines
Authors: Malcolm Jodie R. , Bridge Katherine S. , Holding Andrew N. , Brackenbury William J. 
Published: January 2025

Abstract
Hypoxia is common in breast tumours and is linked to therapy resistance and advanced disease. To understand hypoxia-driven breast cancer progression, RT-qPCR is a widely used technique to quantify transcriptional changes that occur during malignant transformation. Reference genes (RGs) are endogenous RT-qPCR controls used to normalise mRNA levels, allowing accurate assessment of transcriptional changes. However, hypoxia reprograms transcription and post-transcriptional processing of RNA such that favoured RGs including GAPDH or PGK1 are unsuitable for this purpose. To address the need for robust RGs to study hypoxic breast cancer cell lines, we identified 10 RG candidates by analysing public RNA-seq data of MCF-7 and T-47D (Luminal A), and, MDA-MB-231 and MDA-MB-468 (triple negative breast cancer (TNBC)) cells cultured in normoxia or hypoxia. We used RT-qPCR to determine RG candidate levels in normoxic breast cancer cells, removing TBP and EPAS1 from downstream analysis due to insufficient transcript abundance. Assessing primer efficiency further removed ACTB, CCSER2 and GUSB from consideration. Following culture in normoxia, acute, or chronic hypoxia, we ascertained robust non-variable RGs using RefFinder. Here we present RPLP1 and RPL27 as optimal RGs for our panel of two Luminal A and two TNBC cell lines cultured in normoxia or hypoxia. Our result enables accurate evaluation of gene expression in selected hypoxic breast cancer cell lines and provides an essential resource for assessing the impact of hypoxia on breast cancer progression.
Improving diagnostics and surveillance of malaria among displaced people in Africa
Authors: Abdul-Rahman Toufik , Ajetunmobi Oyinbolaji Akinwande , Bamigbade Gafar Babatunde , Ayesiga Innocent , Shah Muhammad Hamza , Rumide Tolulope Sharon , Adesina Abdurahman Babatunde , Adeshina Ganiyat Adekemi , Oni Oluwabusola Elizabeth , Christian Bet-ini Nsikak , Aborode Abdullahi Tunde , Wireko Andrew Awuah , Thaalibi Hala Ibrahim , Abdalla Iman Mustafa , Banimusa Sewar Basheer , Jonathan Justice Ndubuisi , Onifade Isreal Ayobami , Haque Md Ariful 
Published: January 2025

Abstract
African communities that have been forced to leave their homes experience a considerably greater susceptibility to malaria as a result of densely populated living conditions, restricted availability of healthcare, and environmental influences. Internally displaced individuals frequently live in large settlements with restricted availability to drinking water, essential sanitation, and medical services, intensifying the spread of malaria. As a result, the occurrence of malaria is significantly more common among refugees and internally displaced individuals compared to those who are not displaced. This leads to greater rates of illness and death, especially among young people. Insufficient monitoring worsens the condition, leading to delayed identification and medical intervention, and contributing to a higher incidence of severe malaria and deaths. Furthermore, these communities are faced with economic consequences that contribute to the continuation of poverty and the worsening of socio-economic inequalities. Furthermore, the psychological impact of malaria, which is marked by feelings of anxiety and uncertainty, is particularly severe in vulnerable populations such as displaced children and pregnant women, aggravating the overall burden. Hence, addressing malaria in displaced populations in Africa requires comprehensive and well-coordinated strategies. Advanced diagnostic and surveillance technologies are essential for promptly identifying and treating malaria, providing chances to monitor and control its spread effectively. Collaboration among healthcare, policy, and humanitarian sectors is crucial for implementing comprehensive solutions that incorporate enhanced diagnostics, surveillance, and socio-psychological support. Active involvement of the community, usage of Community Health Workers, and regular collection of surveillance data are crucial in increasing awareness, directing control efforts, and tackling the specific difficulties encountered by displaced groups. Moreover, the implementation of environmental management, the incorporation of health services, and the utilization of adaptable healthcare interventions are essential for reducing the effects of malaria. To mitigate the impact of malaria and improve health outcomes among displaced populations in Africa, it is crucial to focus on these specific areas.
HEMA-free versus HEMA-containing adhesive systems: a systematic review
Authors: Abdelkhalek Esraa , Hamama Hamdi H. , Mahmoud Salah H. 
Published: January 2025

Abstract

Background
Hydrophilic monomer 2-hydroxyethyl methacrylate (HEMA)-free adhesive systems are gaining increasing popularity nowadays. Although the addition of HEMA to dental adhesives improves dentin wettability and resin diffusion into demineralized collagen fibrils, HEMA’s high hydrophilicity can lead to hydrolytic degradation of the adhesive interface. Thus, HEMA-free adhesive systems have been developed. Unfortunately, the lack of HEMA in the adhesive composition may lead to a separation phase between hydrophobic and hydrophilic components. The aim of this systematic review was to evaluate the clinical performance of HEMA-free adhesive systems and compare them with HEMA-containing ones.

Methods
An electronic search of The National Library of Medicine (MEDLINE/PubMed) was conducted. Eligibility criteria were reporting empirical data from clinical studies published between 2013 and 2023 about the clinical performance of HEMA-free adhesive systems for direct resin composite restorations. Studies with at least 2-year clinical follow-up done in permanent dentition in any form of cavities were selected. The included studies were assessed for risk of bias using the modified Cochrane Collaboration tool criteria.

Results
The database search returned 147 studies; a total of 7 studies were included in this review; the majority of studies reported no significant difference between the two types of adhesives for the parameter of retention.

Conclusions
HEMA-free adhesive systems exhibited good clinical performance with regard to retention. There was some concern about their influence on marginal adaptation and marginal discoloration due to the conflicted results reported by the included trials. Thus, the results need to be confirmed with long-term evaluations.

Systematic review registration
PROSPERO CRD42023448952.
Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations
Authors: Castillo-Lopez Ezequias , Biber Patrick , Sener-Aydemir Arife , Hummel Karin , Razzazi-Fazeli Ebrahim , Reisinger Nicole , Zebeli Qendrim , Kreuzer-Redmer Susanne , Hartinger Thomas 
Published: January 2025

Abstract

Background
The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G (IgG) content. Immediately after calving, colostrum samples were collected from 18 cows to measure IgG concentration. Based on colostrum IgG content, samples were classified through cluster analysis and were identified as poor, average, and excellent quality. The proteome was assessed with quantitative shotgun proteomics; abundance data were compared among the colostrum types; enrichment analysis of metabolic processes and proteins classes was performed as well. We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves.

Results
On average, 428 proteins were identified per sample, which belonged mainly to cellular process, biological regulation, response to stimulus, metabolic process, and immune system process. Most abundant proteins were complement C3 (Q2UVX4), alpha-S1-casein (P02662), Ig-like domain-containing protein (A0A3Q1M032), albumin (A0A140T897), polymeric immunoglobulin receptor (P81265), lactotransferrrin (P24627), and IGHG1*01 (X16701_4). Colostrum of excellent quality had greater (P < 0.05) abundance of serpin A3-7 (A2I7N3), complement factor I (A0A3Q1MIF4), lipocalin/cytosolic fatty-acid binding domain-containing protein (A0A3Q1MRQ2), complement C3 (E1B805), complement component 4 binding protein alpha (A0AAF6ZHP5), and complement component C6 (F1MM86). However, colostrum of excellent quality had lower (P < 0.05) abundance of HGF activator (E1BCW0), alpha-S1-casein (P02662), and xanthine dehydrogenase/oxidase (P80457). This resulted in enrichment of the biological processes predominantly for complement activation alternative pathway, complement activation, complement activation classical pathway, humoral immune response, leukocyte mediated immunity, and negative regulation of endopeptidase activity in excellent-quality colostrum. Additionally, some colostrum proteins were found to be correlated with the blood globulin level of cows and with the passive immunity level of calves (P < 0.05; r ≥ 0.57).

Conclusions
This study provides new insights into the bovine colostrum proteome, demonstrating associations between IgG levels and the abundance of other proteins, as well as the enrichment of metabolic processes related to innate immune response. Thus, results suggest that the colostrum proteomic profile is associated with the content of IgG. Future research should deeply explore the association of these findings with pre-calving nutrition status and blood composition of the cow, and with passive immunity transfer to the calf.
Extreme time–frequency connectedness between oil shocks and sectoral markets in the United States
Authors: Ozcelebi Oguzhan , Pérez-Montiel Jose , Kang Sang Hoon 
Published: January 2025

Abstract
This study assessed the connectedness between oil shocks and industry stock indexes in the United States (US). We consider the normal and extreme conditions across different frequency horizons, and the quantile time–frequency connectedness method is used to determine the tail risk contagion under different frequency horizons. Our results reveal that the short-term frequency connectedness significantly exceeds the long-term frequency connectedness. We also indicate that the connectedness in the lower and upper quantiles is greater than at the conditional mean. Importantly, oil risk shock is the biggest net transmitter of shocks to the US sectors in normal and extreme conditions, highlighting that oil risk shocks cause substantial variations in US sector stock returns in the short, medium, and long term. Finally, QAR(3) model demonstrates the significant impact of oil risk shocks on US sector stock returns during extreme and normal conditions. Therefore, our study underscores the role of asymmetry in the reaction of US sector stock returns to oil-related shocks, and we suggest that policies aimed at overcoming the adverse effects of oil shocks on stock markets and promoting financial stability should incorporate asymmetric features.
Sleep quality among sample of Egyptian patients with Alzheimer’s disease: risk factors and role of genetic profiling for ACE, ACE 2 and APO E genotypes
Authors: Elbeh Khaled Ahmed , Abbas Mohamed Abdala , Hassan Mohammed H. , Hefny Mohamed Moslem , Sakr Shimaa Fathy , Mohamed Reham Bakry , Bekiet Mahmoud Ahmed , Desoky Tarek 
Published: January 2025

Abstract

Background
Most individuals having Alzheimer’s disease generally experience increased frequency of awakenings as well as prolonged wakefulness periods at night. Brain wave research indicate reductions in both REM (dreaming stage) along with NREM (non-dreaming stage). The inherited pattern of Alzheimer’s disease is attributed to presence of genetic polymorphisms. The association of these polymorphisms and sleep pattern in Alzheimer’s disease patients was not assessed before.

Purpose
This research was aimed at assessing the association between sleep quality as well as genetic polymorphisms of ACE, ACE2 and Apo E among Alzheimer’s disease patients in Upper Egypt.

Patients and methods
Our cross-sectional study with analytic component involved 50 cases having Alzheimer’s disease evaluated utilizing Pittsburg sleep quality index for sleep quality. They were accordingly divided into good as well as poor sleep quality groups. Genetic analysis of ACE, ACE2 (rs2285666) SNPs and Apo E (rs121918398) SNPs was done using (RFLP-PCR) I.

Results
No statistically significant variation was documented among both groups regarding age as well as age at diagnosis. Female patients were more susceptible for poor sleep quality. In addition, patients came from rural areas were significantly higher among poor sleep quality group. Statistical significant variations were documented among both groups as regard presenting symptoms and HAM-A and HAM-D. No statistically significant variations were documented among both groups as regard ACE genotypes; however, ACE 2 AA genotype and A allele were higher with statistically significant difference among poor sleep quality group. Apo E3/E4 genotype and E4 allele were greater with statistically significant variations among poor sleep quality group.

Conclusions
Female patient and rural Alzheimer’s disease patients at high risk for poor sleep quality. In addition, Apo E4 carriers and ACE2 AA carriers are at increasing susceptibility for poor sleep quality.
Integrative transcriptome profiling for identifying ALS potential treatment using the drug repurposing approach
Authors: Kashani Navid , Sabbaghian Amir , EmamiPari Fatemeh , Khalili Gorjimahalleh Mohammad , Aalikhani Mahdi 
Published: January 2025

Abstract

Background
Amyotrophic lateral sclerosis (ALS), an alarming neurodegenerative disorder, induces muscle atrophy and motor deterioration. The current treatments exhibit limited improvement in survival rates. Thus, we here attempted to identify crucial genetic biomarkers through transcriptome profiling and systems biology methodologies to advance our knowledge of the diagnosis and pathogenesis of ALS. Following this, a drug repurposing approach was employed to introduce possible treatments for ALS.

Results
After analyzing differentially expressed genes (DEG) using different in silico approaches, 43 DEGs (23 upregulated and 20 downregulated) were identified, which were abnormally expressed in ALS patients compared to healthy individuals. Two proteins CMPK2 and IFI44L were identified as ALS biomarkers and selected for molecular docking. Then, molecular docking was performed to repurpose drugs that have the potential to suppress upregulated proteins. Accordingly, three drugs including ketoprofen, thalitone, and cromolyn have been repurposed against CMPK2 and IFI44L proteins.

Conclusions
CMPK2 and IFI44L serve as potential biomarkers for ALS and may be applied in the diagnostic assessment of this disorder within the bloodstream of affected individuals. Furthermore, three drugs were proposed as potential therapeutic candidates for ALS with the help of transcriptomics profiling. We advocate for the implementation of these identified pharmacotherapies in animal models of ALS to validate their therapeutic efficacy. This approach also would help narrow down the options to more suitable targets and economically viable treatments.