bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occurrence of entomopathogenic nematodes, Oscheius myriophilus Poinar in Thailand: Preliminary characterization of novel isolates and biological control potential against insect pests
Authors: Onwong Rattanawadee , Sumaya Nanette Hope , Nitjarunkul Achariya , Kerdim Sineenuch , Khwanket Niyaporn , Noosidum Atirach 
Published: July 2023

AbstractNumerous nematodes in the genus Oscheius possess entomopathogenic abilities. These nematodes also kill their insect hosts by releasing their symbiotic bacteria within 48–72 h. In this study, 12 indigenous isolates of Oscheius myriophilus based on molecular, morphological, and morphometric studies were recovered from different undisturbed soils in Thailand. Moreover, the virulence of 12 O. myriophilus at a rate of 25 IJs/cm2 against fifth‐instar larvae of Galleria mellonella ranged from 56%–86% after 72 h of exposure. Oscheius myriophilus EPNKU40 exhibited the highest efficacy in causing mortality in the insect larvae. It also killed third‐instar larvae of Spodoptera litura and Spodoptera exigua up to 60% after 72 h of exposure. The median lethal concentration (LC50) values of O. myriophilus EPNKU40 against G. mellonella larvae after 48 and 72 h of exposure were 11 and 4 IJs/cm2, respectively. The discovery of O. myriophulus is the first record in Thailand characterized using morphological, morphometric, and molecular diagnostic tools, which have shown lethal effects against insect pests and can later be utilized as biocontrol agents after several comprehensive assessments.
The haematology of clinically healthy, farmed juvenile Asian seabass (Lates calcarifer Bloch)—reference intervals, and indicators of subclinical disease
Authors: Chew Xian Zhe , Gibson‐Kueh Susan 
Published: July 2023

AbstractThis study establishes the blood reference intervals (RIs) for clinically healthy and farmed juvenile Asian seabass (Lates calcarifer), within 4–6 weeks after stocking into flow‐through, marine aquaculture systems. The 90% percentile RIs (n = 156, mean bodyweight 41.8 g) are as follows: glucose (GLU) 2.4–11.3 mmol/L, haematocrit (Hct) 18.9%–39.2%, haemoglobin concentration (Hb) 56.0–85.0 g/L, total plasma protein (TPP) 56.0–77.0 g/L, total red blood cell (RBC) count 4.1–11.2 × 1012/L, total white blood cell (WBC) count 5.3–69.9 × 109/L, total lymphocytes 4.7–51.4 × 109/L, monocytes 0.3–16.2 × 109/L and heterophils count 0.6–8.4 × 109/L. Pearson's method analysis showed weak but significantly positive correlations between fish bodyweight and Hct, Hb, TPP and total RBC count (p < 0.05). Histopathology of 42 of the 156 clinically healthy fish used to derive the RIs, with blood values within the 90% percentile range, did not exhibit any abnormal pathology. In contrast, histopathology from a different group of clinically healthy L. calcarifer (n = 72, mean bodyweight 31.3 g) with blood values falling outside of these established 90% percentile RIs showed that 25% of these fish had severe, chronic granulomatous enteritis, and 13% had severely depleted lipid stores in their liver. Point biserial correlation analysis of blood values from this second group of 72 fish showed that elevated total WBC, monocyte and heterophil counts and reduced Hct levels are significantly associated (p < 0.05) with the occurrence of severe, chronic granulomatous enteritis and depleted lipid stores in their liver. Reduced blood GLU and TPP levels in the second group of fish were significantly associated with fish that had depleted lipid stores in liver (p < 0.05), corroborating a period of malnutrition. This study is among the first to establish blood RIs for clinically healthy, farmed juvenile L. calcarifer and detection of subclinical diseases in fish to support early intervention.
Phylogenetic analysis of Shewanella spp. isolated from fish
Authors: Paździor Ewa , Bomba Arkadiusz , Tłuścik Katarzyna , Nadolna‐Ałtyn Katarzyna , Podolska Magdalena , Reichert Michał , Wasyl Dariusz , Pękala‐Safińska Agnieszka 
Published: July 2023

AbstractDifferent species of Shewanella spp. widely inhabit freshwater and marine environments. Some of them are opportunistic fish pathogens. The application of high‐throughput sequencing enabled the characterization and taxonomic reclassification of many Shewanella spp. species. Still, some strains collected from fish need to be better recognized. The aim of the present study was to classify and determine the phylogenetic relationships of Shewanella spp. collected from fish. The complete genomes of 94 strains of Shewanella spp. from different fish species were sequenced using Illumina platform (MiSeq). The 16S rRNA gene, genomic features and whole‐genome relationships of those bacteria were comprehensively analysed in comparison to reference strains. Whole‐genome analysis showed that the tested Shewanella spp. strains were clustered into six groups similar to reference strains of S. xiamenensis, S. oneidensis, S. glacialipiscicola, S. hafniensis, S. baltica and S. oncorhynchi. Our study indicates that the whole‐genome sequence analysis enabled taxonomic classification and assessment of the diversity of the Shewanella spp. strains, as opposed to recently the gold standard method of 16S rRNA amplicon sequencing. The high genetic diversity and low similarity to the reference genome of S. oneidensis indicate that the group of strains may be a subspecies or even new species. Furthermore, we showed that the most frequent Shewanella spp. species occurring in freshwater fish in our study is the recently described species S. oncorhynchi.
Debriefing to improve interprofessional teamwork in the operating room: A systematic review
Authors: Skegg Emma , McElroy Canice , Mudgway Mercedes , Hamill James 
Published: July 2023

AbstractPurposeDebriefing has been pivotal in medical simulation training, but its application to the real‐world operating room environment has been challenging. We reviewed the literature on routine surgical debriefing with special reference to its implementation, barriers, and effectiveness.DesignDescriptive systematic review following the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guidelines.MethodsInclusion criteria were papers pertaining to debriefing in routine surgical practice. Excluded were papers reporting simulation training. We searched Google Scholar, CINAHL, Web of Science Core Collection, PsychINFO, Medline, Embase, and ProQuest Theses & Dissertations Global. The last search was performed on March 14, 2022. Quality was assessed on a 21‐point checklist adapted from a standard reporting guideline. Synthesis was descriptive.FindingsThe search process resulted in 19 papers. Publication dates ranged from 2007–2022. Study methods included surveys, interviews, and analysis of administrative data. Five papers involved a specific intervention. Quality scores ranged from 12–19 out of 21. On synthesis, we identified five topics: explanations of how debriefing had been implemented; the value of coaching and audit; the learning dimensions of debriefing, both team learning and quality improvement at the organizational level; the effect of debriefing on patient safety or the organization's culture; and barriers to debriefing.ConclusionsSuccessful implementation programs were characterized by strong commitment from management and support by frontline workers. Integration with administrative quality and safety processes, and information feedback to frontline workers are fundamental to successful debriefing programs.Clinical RelevanceDebriefing can improve teamwork, learning, and psychological safety but is difficult to practice in the operating room environment. It is relevant to review the benefits and barriers to debriefing, and to learn from the experience of others, in order to run better debriefing models in our own hospitals.
Mechanisms underlying sex bias in oral immune‐mediated conditions, an insight
Authors: Alrashdan Mohammad S. , Al‐Rawi Natheer H. , Hassona Yazan , Al Kawas Sausan , Cirillo Nicola 
Published: July 2023

AbstractThe predilection for women in systemic autoimmune diseases is well established. However, this sex bias in oral autoimmune diseases has been classically reported from an epidemiological perspective without any elaborate attempts to unveil the underlying mechanisms. The unique nature of the oral environment is likely to impose a combination of systemic and local factors that ultimately result in the sex bias in autoimmune diseases of the oral cavity. Variations of immune responses, target organ vulnerability, endocrine and genetic factors, sex chromosomes and modes of parental inheritance are potential systemic factors, while the oral microbiome, oral tolerance, saliva, and oral epithelial stem cells may account for local contributing factors. This review will discuss the preponderance of women in oral immune‐mediated diseases, the potential systemic and local mechanisms underlying this predominance and highlight the crucial need for further research in this area.
Digital workflow for duplication of gold onlay anatomy to monolithic zirconia onlay for a complete denture: A technique paper
Authors: Alfaifi Mohammed A. , Altoman Majed S. , Alqarni Hatem , Gadah Thuraya S. , Kattadiyil Mathew T. 
Published: July 2023

AbstractDigital replication of an existing cast gold onlay anatomy to a more esthetic material for a new complete denture is challenging. A technique is presented that uses digital technology to fabricate a new maxillary complete denture with monolithic zirconia onlays that duplicate the anatomy of an existing complete denture with cast gold onlays.
Psychosocial Factors Associated with Well‐Being and HbA1c of People with Diabetes in Japan: A Structural Equation Model1
Authors: Huai‐Ching Liu I‐Ting , Uchida Yukiko , Joo Erina , Mano‐Usui Fumika , Okura Mizuyo , Chishima Yuta , Ikeda Kaori , Inagaki Nobuya 
Published: July 2023

AbstractThe purpose of this study is to investigate psychosocial factors (independent and interdependent self‐construal, emotional support) that may influence the health and well‐being of people with diabetes in an interdependent culture. We conducted a cross‐sectional survey on outpatients of type 1 and type 2 diabetes mellitus in Japan (N = 180, age 22–88 years, M = 62.48 years), to examine their independent and interdependent self‐construal, perceived emotional support, and well‐being (interdependent happiness). HbA1c data was obtained from their recent health record via their endocrinologists. Correlation analyses and structural equation modeling (SEM) were conducted to examine the relation between these variables. Patients' well‐being correlated positively with age, independent self‐construal, perceived emotional support, and negatively with HbA1c levels, but did not correlate with interdependent self‐construal. SEM showed that, after controlling for age and gender, independence was related to perceived emotional support and well‐being, which were in turn related to lower HbA1c levels. Our research suggests that, for people with diabetes in Japan, independence rather than interdependence is associated with their psychological well‐being and health outcome.
Ultrasonographic measurement of caudal vena cava to aorta ratio during fluid resuscitation of dogs with spontaneous circulatory shock
Authors: Combet‐Curt J. , Pouzot‐Nevoret C. , Cambournac M. , Magnin M. , Nectoux A. , Bonnet‐Garin J. M. , Goy‐Thollot I. , Barthélemy A. 
Published: July 2023

ObjectivesTo describe the change in the caudal vena cava to aorta ratio (CVC:Ao) ratio during fluid resuscitation of circulatory shock in dogs and compare these results with those of the physical examination and blood lactate.Materials and MethodsPerfusion parameters and blood lactate were recorded at admission. An abdominal point‐of‐care ultrasound protocol was performed, during which the caudal vena cava to aorta ratio was measured on the spleno‐renal view. Measurements were performed within 5 minutes before and after a 10 mL/kg crystalloid fluid bolus. Investigators were not blinded to therapeutic interventions.ResultsTwenty‐nine dogs with physical signs of circulatory shock were enrolled. Caudal vena cava to aorta ratios were below reference interval in 28 of 29 dogs. After bolus administration, median caudal vena cava diameter increased by 0.14 cm (0.69 to 0.83 cm) and median aorta diameter increased by 0.03 cm (0.87 to 0.90 cm) and caudal vena cava to aorta ratio returned to within reference range in 65% of dogs (13/29). Bolus administration was associated with an increase in median caudal vena cava to aorta ratio of 0.10 (95% CI:0.05 to 0.16, P=0.0005). Blood lactate did not change significantly. Heart rate and capillary refill time decreased significantly after fluid bolus (heart rate: estimate=−19 bpm, 95% CI:−30 to −8, P=0.002; capillary refill time: estimate=−1.0 s, 95% CI:−1.3 to −0.7, P < 0.0001).Clinical SignificanceIn this population of dogs with circulatory shock, the caudal vena cava to aorta ratio significantly increased after a fluid bolus. Future studies that implement blinding of the outcome assessors are warranted to confirm these findings.
Continuous positive airway pressure and adherence in patients with different endotypes of obstructive sleep apnea
Authors: Cheng Wan‐Ju , Finnsson Eysteinn , Ágústsson Jón S. , Sands Scott A. , Hang Liang‐Wen 
Published: July 2023

SummaryDetermining the endotypes of obstructive sleep apnea (OSA) has potential implications for precision interventions. Here we assessed whether continuous positive airway pressure (CPAP) treatment outcomes differ across endotypic subgroups. We conducted a retrospective analysis of data obtained from 225 patients with moderate‐to‐severe OSA from a single sleep centre. Polysomnographic and CPAP titration study data were collected between May 2020 and January 2022. One‐month CPAP treatment adherence was followed. Obstructive sleep apnea endotypes, namely arousal threshold, collapsibility, loop gain, and upper airway gain were estimated from polysomnography and dichotomised as high versus low. We examined associations between endotypic subgroups and (1) optimal CPAP titration pressure, (2) CPAP‐related improvements in sleep architecture (proportions of slow‐wave and rapid eye movement (REM) sleep), and (3) CPAP adherence. We observed that patients with high collapsibility required a higher CPAP pressure than those with low collapsibility (∆ = 0.4 cmH2O, 95% confidence interval [CI] = 0.3–1.7). A larger increase in slow‐wave sleep and in REM sleep proportions after CPAP treatment were observed in patients with a high arousal threshold, high collapsibility, high loop gain, or high upper airway gain than in those with low levels of endotypes. High loop gain and high collapsibility were independently associated with longer CPAP use hours per night (∆ = 0.6 h, 95% CI = 0.2–1.5 and ∆ = 0.3 h, 95% CI = 0.03–1.5, respectively). In conclusion, different endotypic subgroups of OSA exhibit a difference in outcomes of CPAP treatment. Knowledge of endotypes may help clinicians to understand which patients are expected to benefit most from CPAP therapy prior to its administration.
The NAFLD burden on mortality and morbidities in general population: A community‐based longitudinal study (NASH‐CO study)
Authors: Nabi Oumarou , Lapidus Nathanaël , Boursier Jerome , de Ledinghen Victor , Kab Sofiane , Renuy Adeline , Zins Marie , Serfaty Lawrence , Lacombe Karine 
Published: July 2023

AbstractBackgroundThe impact of non‐alcoholic fatty liver disease (NAFLD) on morbidity and mortality has yet to be documented at the general population level. This study aimed to assess whether NAFLD was associated with morbidities and mortality and to estimate its impact on health status and mortality.MethodsThe study population consisted of 137 206 participants from Constances cohort. Non‐invasive diagnosis of NAFLD and advanced fibrosis was performed using the fatty liver index and Forns index, respectively. Constances data were linked to health care and hospitalization data to identify liver‐related events, cardiovascular diseases (CVD), extrahepatic cancers (EHC), chronic kidney disease (CKD) and all‐cause mortality.ResultsThe prevalence of NAFLD was 18.3% in subjects without other chronic liver diseases, among whom 2.7% had fibrosis. NAFLD after IPTW‐weighted remained associated with an increased risk of death (HR 1.26, 95% CI 1.01–1.57), hepatic‐related complications (HR 2.48, 95% CI 1.99–3.29), CVD (HR 1.42, 95% CI 1.30–1.55), EHC (HR 1.11, 95% CI 1.01–1.28) and CKD (HR 1.81, 95% CI 1.53–2.07) compared to those without chronic liver diseases risk factors (Non‐NAFLD). In the trend analysis over the study period of inclusion and compared to Non‐NAFLD, NAFLD has shown a fastest growing cause of hepatic events (HR 1.38, 95% CI 1.07–1.76 per year), CVD (HR 1.08, 95% CI 1.03–1.12), CKD (HR 1.16, 95% CI 1.07–1.25), and death (HR 1.39, 95% CI 1.39–1.50).ConclusionThis large community‐based cohort showed that NAFLD was associated with excess morbidity and mortality and demonstrated a fastest‐growing trend.
Recurrent allopolyploidizations diversify ecophysiological traits in marsh orchids (Dactylorhiza majaliss.l.)
Authors: Wolfe Thomas M. , Balao Francisco , Trucchi Emiliano , Bachmann Gert , Gu Wenjia , Baar Juliane , Hedrén Mikael , Weckwerth Wolfram , Leitch Andrew R. , Paun Ovidiu 
Published: July 2023

AbstractWhole‐genome duplication has shaped the evolution of angiosperms and other organisms, and is important for many crops. Structural reorganization of chromosomes and repatterning of gene expression are frequently observed in allopolyploids, with physiological and ecological consequences. Recurrent origins from different parental populations are widespread among polyploids, resulting in an array of lineages that provide excellent models to uncover mechanisms of adaptation to divergent environments in early phases of polyploid evolution. We integrate here transcriptomic and ecophysiological comparative studies to show that sibling allopolyploid marsh orchid species (Dactylorhiza, Orchidaceae) occur in different habitats (low nutrient fens vs. meadows with mesic soils) and are characterized by a complex suite of intertwined, pronounced ecophysiological differences between them. We uncover distinct features in leaf elemental chemistry, light‐harvesting, photoprotection, nutrient transport and stomata activity of the two sibling allopolyploids, which appear to match their specific ecologies, in particular soil chemistry differences at their native sites. We argue that the phenotypic divergence between the sibling allopolyploids has a clear genetic basis, generating ecological barriers that maintain distinct, independent lineages, despite pervasive interspecific gene flow. This suggests that recurrent origins of polyploids bring about a long‐term potential to trigger and maintain functional and ecological diversity in marsh orchids and other groups.
Relic DNA obscures DNA‐based profiling of multiple microbial taxonomic groups in a river‐reservoir ecosystem
Authors: Xue Yuanyuan , Abdullah Al Mamun , Chen Huihuang , Xiao Peng , Zhang Hongteng , Jeppesen Erik , Yang Jun 
Published: July 2023

AbstractNumerous studies have investigated the spatiotemporal variability in water microbial communities, yet the effects of relic DNA on microbial community profiles, especially microeukaryotes, remain far from fully understood. Here, total and active bacterial and microeukaryotic community compositions were characterized using propidium monoazide (PMA) treatment coupled with high‐throughput sequencing in a river‐reservoir ecosystem. Beta diversity analysis showed a significant difference in community composition between both the PMA untreated and treated bacteria and microeukaryotes; however, the differentiating effect was much stronger for microeukaryotes. Relic DNA only resulted in underestimation of the relative abundances of Bacteroidota and Nitrospirota, while other bacterial taxa exhibited no significant changes. As for microeukaryotes, the relative abundances of some phytoplankton (e.g. Chlorophyta, Dinoflagellata and Ochrophyta) and fungi were greater after relic DNA removal, whereas Cercozoa and Ciliophora showed the opposite trend. Moreover, relic DNA removal weakened the size and complexity of cross‐trophic microbial networks and significantly changed the relationships between environmental factors and microeukaryotic community composition. However, there was no significant difference in the rates of temporal community turnover between the PMA untreated and treated samples for either bacteria or microeukaryotes. Overall, our results imply that the presence of relic DNA in waters can give misleading information of the active microbial community composition, co‐occurrence networks and their relationships with environmental conditions. More studies of the abundance, decay rate and functioning of nonviable DNA in freshwater ecosystems are highly recommended in the future.
Negative regulation of MurZ and MurA underlies the essentiality of GpsB‐ and StkP‐mediated protein phosphorylation in Streptococcus pneumoniaeD39
Authors: Tsui Ho‐Ching Tiffany , Joseph Merrin , Zheng Jiaqi J. , Perez Amilcar J. , Manzoor Irfan , Rued Britta E. , Richardson John D. , Branny Pavel , Doubravová Linda , Massidda Orietta , Winkler Malcolm E. 
Published: July 2023

AbstractGpsB links peptidoglycan synthases to other proteins that determine the shape of the respiratory pathogen Streptococcus pneumoniae (pneumococcus; Spn) and other low‐GC Gram‐positive bacteria. GpsB is also required for phosphorylation of proteins by the essential StkP(Spn) Ser/Thr protein kinase. Here we report three classes of frequently arising chromosomal duplications (≈21–176 genes) containing murZ (MurZ‐family homolog of MurA) or murA that suppress ΔgpsB or ΔstkP. These duplications arose from three different repeated sequences and demonstrate the facility of pneumococcus to modulate gene dosage of numerous genes. Overproduction of MurZ or MurA alone or overproduction of MurZ caused by ΔkhpAB mutations suppressed ΔgpsB or ΔstkP phenotypes to varying extents. ΔgpsB and ΔstkP were also suppressed by MurZ amino‐acid changes distant from the active site, including one in commonly studied laboratory strains, and by truncation or deletion of the homolog of IreB(ReoM). Unlike in other Gram‐positive bacteria, MurZ is predominant to MurA in pneumococcal cells. However, ΔgpsB and ΔstkP were not suppressed by ΔclpCP, which did not alter MurZ or MurA amounts. These results support a model in which regulation of MurZ and MurA activity, likely by IreB(Spn), is the only essential requirement for StkP‐mediated protein phosphorylation in exponentially growing D39 pneumococcal cells.
Escherichia coliDNA repair helicase Lhr is also a uracil‐DNA glycosylase
Authors: Buckley Ryan J. , Lou‐Hing Anna , Hanson Karl M. , Ahmed Nadia R. , Cooper Christopher D. O. , Bolt Edward L. 
Published: July 2023

AbstractDNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well‐characterized DNA repair helicases that are fused to additional 600–700 amino acids of unknown function, but with structural homology to SecB chaperones and AlkZ DNA glycosylases. Here, we identify that Escherichia coli Lhr is a uracil‐DNA glycosylase (UDG) that depends on an active site aspartic acid residue. We show that the Lhr DNA helicase activity is functionally independent of the UDG activity, but that the helicase domains are required for fully active UDG activity. Consistent with UDG activity, deletion of lhr from the E. coli chromosome sensitized cells to oxidative stress that triggers cytosine deamination to uracil. The ability of Lhr to translocate single‐stranded DNA and remove uracil bases suggests a surveillance role to seek and remove potentially mutagenic base changes during replication stress.