bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fecal carriage and molecular epidemiology of carbapenem-resistant Enterobacteriaceae isolated from children in Henan Province, China
Authors: Yu Jing , Pan Sijia , Tai Shuhong , Zhang Chengwen , Yuan Enwu , Duan Yitao 
Published: February 2023

Abstract
Background: This study sought to investigate the carrier rate of intestinal carbapenem-resistant Enterobacteriaceae (CRE) in children, in Henan Province.
Methods: In a prospective study, 1019 non-repetitive fecal samples were consecutively obtained from outpatient (n = 710) and inpatient children (n = 309). Isolates were cultured in MacConkey medium from fecal specimens, and matrix-assisted laser desorption ionization-time of flight mass spectrometry was used for strain identification. Imipenem and meropenem E-test strips were used for CRE screening, and antimicrobial susceptibility was determined using VITEK-2 AST GN335. Resistance genes and multilocus sequence types were identified by whole-genome sequencing.
Results: The overall CRE colonization rates in children were 1.5%. Five CRE were isolated from outpatient children with a colonization rate of 0.7%. Strains were mainly NDM-5 producing Escherichia coli, which belonged to ST410, ST1193, and ST10885. Ten CRE were isolated from hospitalized children with a colonization rate of 3.2%, mainly NDM-producing K. pneumoniae and NDM-5-producing E. coli. The NDM-producing K. pneumoniae belonged to subtypes, including ST70, ST357, ST491, and ST2864. The blaNDM-5 gene was the major carbapenemase gene harbored by gastrointestinal CRE strains, followed by blaKPC-2. Furthermore, extended-spectrum b-lactamase genes (blaCTX-M, blaTEM, and blaSHV) and AmpC genes (blaDHA-1 and blaCMY-2) were detected. All CRE strains showed high resistance to most of the routinely used antibiotics, except for polymyxin B and tigecycline.
Conclusions: The intestinal CRE colonization rate of children in Henan is very low, and the production of blaNDM carbapenemase was the main mechanism of carbapenem resistance. Different from the main epidemic types of clinically isolated CRE strains, the types of CRE colonizing isolates show diversity.
Comparison of chloroplast genomes of Calypsoinae species (Orchidaceae) living on different lifeforms
Authors: Chen Xiaoyuan , Xiang Xiaoguo , Liu Xuedie , Li Weiyi , Wu Xinchen , Zhou Yadong , Yang Boyun , Luo Huolin 
Published: February 2023

Abstract
Background
The vast majority of angiosperms obtain carbon sources through photosynthesis, while a few Mycoeterotropic plants rely entirely on symbiotic fungi to provide nutrition. The origin and evolution of Mycoeterotropic plant has always been the focus of botanists. Calypsoinae, a common subfamily of Epidendroideae within Orchidaceae, possesses abundant variation in morphology and living habits. Such variation includes that between autotrophy and heterotrophy, a notable discernment that may be exploited to investigate the chloroplast genome evolution of different trophic types of orchids. In this study, the complete chloroplast genome of Danxiaorchis yangii, a heterotrophic orchid, was newly sequenced using illumina HiSeq X ten platform followed by de novo assembly. Moreover, we conducted comparative analysis on 25 Calypsoinae species’ complete chloroplast (CP) genomes, providing insight to explore the chloroplast genome evolution of non-autotrophic plants.
Results
The Danxiaorchis yangii CP genome size is 95,156 bp in length encoding a total of 79 different genes. The CP genomes of 25 Calypsoinae species are typically 88–160 kb in length, while that of the genus Danxiaorchis was typically less than 100 kb, which is consistent with the significantly smaller LSC region of the genus Danxiaorchis. In terms of genome loss, both autotrophic and heterotrophic groups have gene loss phenomena, mainly ndh family loss. Heterotrophic orchid gene loss is more serious, wherein most of the genes associated with photosynthesis have different degrees of deletion. The chloroplast genome of autotrophic orchids was completely collinear without rearrangement or inversion, contrasted with heterotrophic Danxiaorchis, which showed rearrangement and inversion.
Conclusion
This study reported the chloroplast genome sequence of D. yangii, and interpreted its structure and gene number of the chloroplast genome. In addition, by comparing the chloroplast genomes of autotrophic orchids and heterotrophic orchids in Calypsoinae, we found an interesting correlation between the nutrition mode and the chloroplast genome structure. The present study increases understanding of the the evolutionary relationships between heterotrophic orchid and autotrophic plants.
Lottery Ticket Search on Untrained Models With Applied Lottery Sample Selection
Authors: Bluteau Ryan , Gras Robin 
Published: February 2023

Abstract
In our recent paper [1] we presented leading results in tabular datasets by applying the lottery ticket hypothesis to tabular neural networks. However, we were required to train the original large-sized model to find these lottery tickets. In this paper we eliminate the need to train the original model and discover lottery tickets using networks a fraction of the model’s size. Moreover, we show that we can remove up to 95% of the training dataset to discover lottery tickets, while still maintaining similar accuracy. The approach uses a genetic algorithm (GA) to train candidate pruned models by encoding the nodes of the original model for selection measured by performance and weight met-rics. We found that the search process does not require a large portion of the training data, but when the final pruned model is selected it can be retrained on the full dataset, even if it is often not required. We propose a lottery sample hypothesis similar to the lottery ticket hypotheses where a subsample of lottery samples of the training set can train a model with equivalent performance to the original dataset. We show that the combination of finding lottery samples alongside lottery tickets can allow for faster searches and greater accuracy.
A comparative study of robotic surgery and thoracoscopic surgery for mediastinal cysts
Authors: Hong Ziqiang , Sheng Yannan , Cui Baiqiang , Bai Xiangdou , Cheng Tao , Lu Yingjie , Wu Xusheng , Jin Dacheng , Gou Yunjiu 
Published: February 2023

Abstract
Objective: To compare the efficacy and safety of robotic-assisted thoracoscopic surgery (RATS) with video-assisted thoracoscopic surgery (VATS) in the treatment of mediastinal cysts.
Methods: Retrospective analysis on clinical data of 70 cases of minimally invasive surgery for mediastinal cysts completed in the Department of Thoracic Surgery, Gansu Provincial People's Hospital from April 2014 to December 2022. There were 34 cases in the RATS group with a cyst diameter of (3.70±1.16) cm and 36 cases in the VATS group with a cyst diameter of (4.07±1.20) cm. All cysts were evaluated preoperatively using MRI or chest CT localization. Surgery-related indices were compared among the two groups.
Results: All patients in both two groups successfully completed resection of mediastinal cysts without perioperative deaths. Compared with the VATS group, the RATS group possessed shorter operative time [(75.32±17.80) min vs. (102.22±19.80) min, P<0.001], lesser intraoperative bleeding [(10.29±6.39) ml vs. ±1.37) ml vs. (3.08±1.08) ml, P=0.004], and shorter postoperative hospital stay [(3.26±1.36) d vs. (4.64±1.13) d, P=0.001]. There was no statistically significant discrepancy in intermediate openings and complications in both two groups (P>0.05).
Conclusion: Compared with VATS, RATS is safer and more effective in the treatment of mediastinal cysts and thus has advantages in operative time, intraoperative bleeding, postoperative chest drainage time and postoperative hospital stay.
Cytogenetic profiles of a large cohort of patients with sexual developmental disorders; A 22-year single-center experience
Authors: Demirhan Osman 
Published: February 2023

Abstract
Background
Disorders of Sex Development (DSD) are rare and variable disorders that result from abnormalities in karyotype, gonadal formation, androgen synthesis and androgen action. It is important that newborns with gender ambiguity should be evaluated urgently, and then the etiology should be determined by karyotype and hormone analysis.
Objective
The aim of this study is to determine the frequency and structure of cromosomal abnormalities (CAs) seen in patients with the clinical findings of ambiguous genitalia (AG), hypogonadism (HG), intersex (IS), hypospadias (HS), testicular feminization (TF) and vaginal hypoplasia (VH) between 1990 and 2012.
Materials and Methods
We investigated 85 patients which referred to our department. For chromosome analysis, peripheral blood samples were cultured, harvested and banded according to standard methods.
Results
Percentage rates of 117 patients were 53.8%, 27.4%, 8.5%, 5.1%, 3.4% and 1.7%, respectively, having AG, HG, HS, IS, TF and VH irregularities. Of the patients, 64.9% had normal karyotype and 35.1% had abnormal chromosome setup. In 17 (15.3%) of all patients, the phenotypic sex did not match with the genotypic sex (46,XX; 46,XY). Sex-chromosome mismatch chimerism was found in 7 patients (6.0%) (46,XX/46,XY chimeric individuals). Sex chromosome mismatch chimerism was detected in seven patients (5.9%). Sixteen (13.7%) of all patients had mosaicism of the sex chromosomes. Structural abnormalities were found in gonosomal and autosomal chromosomes in 8 patients (6.3%)
Conclusion
The present date shows that CAs play a role in 38.9% of 85 patients with DSD. Molecular and hormonal techniques may also need to be performed in patients whose genotype-phenotype correlations cannot be made in other patients. It also shows that patients with mosaic cytogenetic findings may actually have chimerism and it is difficult to predict the clinical outcome in these patients.
Anxiety, positive anti-SSA/RO52 antibodies, and anti-histone antibodies are putative risk factors for sleep disorders in patients with systemic lupus erythematosus
Authors: Chen Jinxia , Deng Tongliang , Xu Yongzhi , Liu Huafeng , Xie Tong 
Published: February 2023

Abstract
Sleep disorders are prevalent in patients with systemic lupus erythematosus (SLE) and result in an increased risk of chronic diseases, alter host defense and immune mechanisms, and impact the quality of life and cognitive function. Therefore, we explored the risk factors for sleep disorders in patients with SLE to aid the early screening of patients to facilitate early intervention. Patients admitted to the Department of Rheumatology and Immunology, Affiliated Hospital of Guangdong Medical University, from January 2020 to January 2022, and met the inclusion criteria, were enrolled in our study. Pittsburgh Sleep Quality Index (PSQI) scores were used to divide patients into control group (CG; PSQI ≤ 7) and sleep disorder group (SDG; PSQI > 7). A multifactorial binary logistic regression analysis model was used to analyze the risk factors, including demographic characteristics, clinical data, laboratory test results, and psychological and fatigue status. Among the enrolled 203 SLE patients (mean age, 32.2 ± 8.4 years; 11 men and 192 women), 92 cases (45.32%) had sleep disorders. The SDG had more patients living in rural areas, married, unemployed, chronic illness, arthralgia, depression, anxiety, and no fatigue (all P < 0.05); they also had a relatively low hemoglobin concentration (P = 0.005), and elevated anti-histone, anti-SSA/RO60, and anti-SSA/R052 antibodies (P < 0.05). Fatigue (hazard ratio [HR] = 0.41, P = 0.007) was a protective factor, and anxiety (HR = 14.86, P = 0.001), positive anti-SSA/RO52 antibody (HR = 2.71, P = 0.002), and anti-histone antibody (HR = 2.24, P = 0.023) were independent risk factors. Collectively, we concluded that anxiety and positive anti-SSA/RO52 and anti-histone antibodies are independent risk factors for sleep disorders in patients with SLE.
Connection Between Knowledge Management Processes and Sustainability at the Higher Education Institutions
Authors: Budur Taylan , Abdullah Hariem , Rashid Chnar Abdullah , Demirer Halil 
Published: February 2023

Abstract
Purpose— This study attempts to investigate the connection between knowledge management processes and organizational sustainability in higher education sector of a developing region. It is also planned to examine the moderating effect of online trainings in the relationship between knowledge management and college sustainability.
Design, methodology, approach— Data for the study was gathered from several private and public universities in the Kurdistan Region using a survey questionnaire and a random sample technique. The proposed model has been evaluated using 165 appropriate responses. The demographics of the sample is covered by methodology, along with confirmatory factor analysis, structural equation modelling, mediation, and moderation analysis.
Findings— The findings confirm that knowledge codification/storage and knowledge generation have relatively stronger effects on sustainability of the higher education institutions in the Region. Further, staff trainings increase the understanding of the knowledge management processes that knowledge utilization positively boost sustainability of the institutions through moderation of the workshops. It is also found that knowledge creation and development have a favorable direct effect on other knowledge management processes, implying that higher education institutions should utilize appropriate tools to collect and generate information in order to enhance other KM tools.
Originality/value— The results of this study potentially expand the understanding of the knowledge management processes and their effects on the sustainability at the universities of developing countries, specifically, a developing region that attempts to parallel its higher education system with the contemporary developments in the field.
Effect genotoxic, mutagenic and modulate the proteome from the exposition of alternative fuels.
Authors: Londoño Maritza , Isaza Juan Pablo , Trujillo isabel Cristina Ortiz , lópez Juan Bautista , Pedroza Nini Johanna , Vélez veronica Estrada 
Published: February 2023

Abstract
To determine the genotoxic, mutagenic effect and the ability to modulate the organic material extractable from diesel particulate matter mixed with alcohols (10% ethanol and butanol) (MOE), DNA damage was evaluated through the comet assay and mutagenicity. Using the AMES test on Salmonella typhimurium, proteomic analysis was carried out by extracting total proteins from cell cultures, integrity was assessed by one-dimensional (1D) electrophoresis, and protein profiles were obtained by two-dimensional (2D) electrophoresis. Image analysis in PDQuest reflected differentially expressed proteins which were characterized by mass spectrometry (MALDI-TOF/TOF), the protein-protein interaction network was identified with the STRING database, followed by complex detection analysis. through which it was possible to clarify the cell phones involved. The results showed that MOE caused DNA damage and mutagenicity in bacteria, and the Cox5A, TPI1, PRDX2, ERP29 and PDIA3 proteins were overexpressed and involved in processes related to oxidative stress and oxidative detoxification. In conclusion, exposure to these treatments modulates the proteome of the HepG2 cell line, evidencing detoxification processes at the cellular level.
Hospitalization costs among Hospital-Acquired Venous Thromboembolism patients in China: a cross-sectional study
Authors: Liu Tong , Fu Mei-Ling , Huang Huan-Huan , Zhao Qing-Hua , Li Xue-Lian , XIAO Ming-Zhao , Chen Deng-Ju 
Published: February 2023

Abstract
Background
In this study, we aimed to analyze the hospitalization costs for Hospital-Acquired Venous Thromboembolism(HA-VTE) patients in The First Affiliated Hospital of Chongqing Medical University from January 2019 through December 2021, and to determine the factors associated with hospitalization costs.
Methods
The information of 519 HA-VTE inpatients was collected from the Electronic Medical Record System of the hospital. Univariate and Linear regression analysis were employed to evaluate the association between hospitalization cost and its related factors.
Results
A total of 519 patients with HA-VTE were included and analyzed, including 319 patients with DVT, 60 patients with PE, and 68 patients with DVT&PE. the mean length of stay (LoS) of HA-VTE patients was 18.55 ± 19.02 days, and the mean hospital cost was RMB 9,047.57 ± 15,793.04, with a median of RMB 4435.95 (IQR 2760.87,8753.76). For patients with DVT, PE, and DVT&PE, the mean length of stay (LoS) was 18.26 ± 18.71 days, 16.37 ± 15.21 days, and 22.16 ± 23.20 days, respectively, and the mean hospital costs were RMB 6412.08 ± 8827.82, RMB 11423.11 ± 11897.64, and RMB 22105.57 ± 33636.74. As for cost composition analysis, medical service fees in accounted for more than 30% of the hospitalization costs, laboratory and tests and material fees were the second and third largest percentages of the hospitalization costs, respectively. Linear regression analysis showed that Subtype of VTE, LoS, Number of surgery and Payment Type were the key determinants of hospitalization costs for all HA-VTE patients.
Conclusions
We found that hospitalization costs were significantly higher in patients with DVT & PE than in patients with DVT & PE, and medical service costs were the largest contributor to hospitalization costs, probably because most patients required surgical treatment and more general treatment and care. LoS and subtype of VTE may significantly affect hospitalization costs, and it is recommended to increase the reimbursement rate by medical insurance to prevent complications, Reducing LoS and improving treatability may help to reduce the financial burden of HA-VTE patients in China.
Effect of bedside health education for elderly patients with fragility fracture by specialist physicians on the diagnosis and treatment of osteoporosis during hospitalization and the visiting rate to osteoporosis clinic after discharge in a high volume orthopedic hospital
Authors: Yang Jiancheng , Tang Qinghua , Che Maohong , Shi Jie , Yang Lei , Zeng Yuhong 
Published: February 2023

Abstract
Summary We retrospectively analyzed 12999 elderly patients with fragility fracture and found that the detection rate of bone mineral density (BMD) and bone turnover markers (BTMs), the treatment rate of osteoporosis, and the visiting rate to the osteoporosis specialist clinic after discharge are significantly enhanced in fragility fracture patients after receiving health education on osteoporosis related knowledge during hospitalization.
Purpose To observe the effect of health education on the diagnosis and treatment of osteoporosis during hospitalization and the rate of come back to osteoporosis clinic after discharge in elderly patients with fragility fracture.
Methods A retrospective analysis was performed on 12999 elderly patients with fragility fracture admitted to Xi'an Honghui Hospital from March 2021 to December 2022. The patients were divided into the health education group and the non-health education group according to whether they received health education on osteoporosis related knowledge during hospitalization. The diagnosis and treatment of osteoporosis during hospitalization and the outpatient treatment of osteoporosis after discharge were compared between the two groups.
Results Among the 7784 patients in the health education group, 4551 (58.47%) received BMD test, 798 (10.25%) received BTMs test, the treatment rate of osteoporosis was 53.16%, and 1232 (15.83%) came back to the osteoporosis specialist clinic after discharge. Among the 5215 patients in the non-health education group, 681 (13.06%) received BMD test, 6 (0.12%) received BTMs test, the treatment rate of osteoporosis was 47.49%, and 440 (8.44%) came back to the osteoporosis specialist clinic within one month after discharge.
Conclusion The education of osteoporosis related knowledge for patients with fragility fracture contribute to enhance the detection rate of BMD and BTMs and the treatment rate of osteoporosis during hospitalization, and increase the rate of coming back to the osteoporosis clinic after discharge.
Human thermal bioclimate of the Swiss-Austrian alpine region based on estimates of the clothing thermal resistance model
Authors: Szalkai Zsófia , Ács Ferenc , Zsákai Annamária 
Published: February 2023

Abstract
Human thermal bioclimate of the Swiss-Austrian alpine region in the period 1991–2020 is simulated by using a clothing thermal resistance model. Clothing thermal resistance (rcl) is estimated for people that are either walking or standing. Climatic data are taken from the ERA5-Land dataset. The human anthropometric data used in the simulations correspond to that of a person with a body mass index of 25 kgm-2. The most important results of the study are as follows: 1) The rcl values are around 0–0.5 clo in the lower areas of the region in July, regardless of whether people are walking or standing. This thermal load causes a "neutral" thermal perception in the vast majority of people. Concerning the swiss region, it is the surroundings of Lake Geneva and the southernmost areas of Canton of Ticino that have the smallest heat deficit, while in Austria these are the vicinity of Lake Neusiedl. 2) In July, in the high mountains, the highest rcl values for a standing person are around 0.4–0.8 clo. 3) In January, the highest rcl values for a standing person can even reach 6 clo. In these cases the difference between the rcl values of standing and walking people can be as much as 2.5–3 clo. The main message of this study is that human thermal bioclimate maps can be used as climate maps, regardless of the fact that climate is not characterized in terms of water supply.
Effectiveness of an educational program on awareness of breast cancer risk factors, symptoms, and barriers to seeking medical help among adolescent Omani school students - An interventional study
Authors: Al-Hosni Khadija , Chan Moon Fai , Al-Azri Mohammed 
Published: February 2023

Abstract
Purpose
Women in Oman are usually diagnosed with BC at a younger age and more advanced stage, with poor five-year survival. This study aimed to evaluate the effectiveness of a BC-related educational program among female Omani adolescents.
Methods
Six female-only public schools were randomly selected from three governorates of Oman and assigned to the control or interventional group. An Arabic version of the Breast Cancer Awareness Measure questionnaire was used to evaluate students attending grades 10 and 11 at baseline (T0) and after 4 weeks (T1). After T0, the intervention group participated in a one-hour BC education program involving group discussions, a slideshow presentation, leaflets, and online access to program materials and videos.
Results
A total of 1,106 students participated, of which 547 (49.5%) and 559 (50.5%) were allocated to the control and intervention groups, respectively. Recognition of BC risk factors (Z = 18.67; p ˂0.001) and symptoms (Z = 20.01; p ˂0.001) increased significantly in the intervention group between T0 and T1 and compared to the control group at T1 (U = 27.27; p ˂0.001, and U = 25.75; p ˂0.001, respectively). Anticipated time to seeking medical help (Z = 18.67; p ˂0.001) and barriers to help-seeking (Z = 7.91; p < 0.001) decreased significantly between T0 and T1 in the intervention group and compared to the control group at T1 (U = 15.78; p ˂0.001, and U = 3.44; p = 0.001, respectively).
Conclusion
The program increased knowledge of BC risk factors and symptoms and promoted early medical help-seeking among Omani female adolescents.
Improving patient safety during position changes under general anaesthesia: “CHOPP check”- a new patient safety and educational tool.
Authors: Hussein Kareem , Lal Shankar , Leonard Irene , Moore Michael 
Published: February 2023

Abstract
The requirement to change position whilst under general anaesthesia may expose patients to significant risk. We devised and implemented a concise and comprehensive patient positioning safety tool with the aim of reducing risk and improve patient safety during position changes under anaesthesia.