bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Nanovaccine Based on Adjuvant Peptide FK‐13 and l‐Phenylalanine Poly(ester amide) Enhances CD8+ T Cell‐Mediated Antitumor Immunity
Authors: Xie Chunyuan , You Xinru , Zhang Hongxia , Li Jiahui , Wang Liying , Liu Yongxiang , Wang Zining , Yao Ruhui , Tong Tong , Li Mengyun , Wang Xiaojuan , Cui Lei , Zhang Huanling , Guo Hui , Li Chunwei , Wu Jun , Xia Xiaojun 
Published: May 2023

AbstractCancer vaccines have shown promise as effective means of antitumor immunotherapy by inducing tumor antigen‐specific T cell immunity. In this study, a novel peptide‐based tumor nanovaccine that boosts antigen presentation and elicits effective antitumor immunity is developed. The adjuvant characteristics of an antimicrobial peptide‐derived core peptide, FK‐13, are investigated and used it to generate a fusion peptide named FK‐33 with tumor antigen epitopes. l‐phenylalanine‐based poly(ester amide) (Phe‐PEA), 8p4, is also identified as a competent delivery vehicle for the fusion peptide FK‐33. Notably, the vaccination of 8p4 + FK‐33 nanoparticles (8FNs) in vivo induces dendritic cell activation in the lymph nodes and elicits robust tumor antigen‐specific CD8+ T cell response. The nanovaccine 8FNs demonstrate significant therapeutic and prophylactic efficacy against in situ tumor growth, effectively inhibit tumor metastasis, and significantly prolong the survival of tumor‐bearing mice. Moreover, 8FNs can incorporate different tumor antigens and exhibit a synergistic therapeutic effect with antiprogrammed cell death protein 1 (PD‐1) therapy. In summary, 8FNs represent a promising platform for personalized cancer vaccines and may serve as a potential combinational modality to improve current immunotherapy.
Multi‐Functional Bio‐HJzyme: Revolutionizing Diabetic Skin Regeneration with its Glucose‐Unlocked Sterilization and Programmed Anti‐Inflammatory Effects
Authors: He Miaomiao , Wang Zuyao , Yang Hang , Wang Qiancun , Xiang Danni , Pang Xinyan , Chan Yau Kei , Sun Dan , Yin Guangfu , Yang Weizhong , Deng Yi 
Published: May 2023

AbstractAntibacterial dynamic therapy (ADT) triggered by reactive oxygen species (ROS) is promising for diabetic infectious disease treatment. However, the limited local O2/H2O2 production and post‐treatment inflammation remain long‐standing issues. To address these challenges, a novel H2‐evolving bio‐heterojunction enzyme (Bio‐HJzyme) consisting of graphite‐phase carbon nitride/copper sulfide (CN/Cu2−xS) heterojunction and glucose oxidase (GOx) is created. The Bio‐HJzyme offers glutathione peroxidase (GPx), peroxidase (POD), and catalase (CAT) mimetic activities; provides anti‐pathogen properties via programmed light activation; and effectively promotes diabetic wound healing. Specifically, its GPx‐mimetic activity and the presence of GOx significantly enhance the yield of H2O2, which can be catalyzed through POD‐mimetic activity to produce highly germicidal •OH. The H2O2 can also be catalyzed to H2O and O2, assisted by the CAT‐mimetic activity. The catalyzed products can then be catalyzed into germicidal •OH and •O2− under NIR light irradiation, giving enhanced ADT. Further, CN can split water to form H2 under solar light, which dramatically suppresses the inflammation caused by excessive ROS. In vivo evaluation confirms that Bio‐HJzyme promotes the regeneration of diabetic infectious skin through killing bacteria, enhancing angiogenesis, promoting wound bed epithelialization, and reinforcing anti‐inflammatory responses; hence, providing a revolutionary approach for diabetic wounds healing.
Additively Manufactured Dual‐Faced Structured Fabric for Shape‐Adaptive Protection
Authors: Tian Yuanyuan , Chen Kaijuan , Zheng Han , Kripalani Devesh R. , Zeng Zhuohong , Jarlöv Asker , Chen Jiayao , Bai Lichun , Ong Adrian , Du Hejun , Kang Guozheng , Fang Qihong , Zhao Lihua , Qi H. Jerry , Wang Yifan , Zhou Kun 
Published: May 2023

AbstractFabric‐based materials have demonstrated promise for high‐performance wearable applications but are currently restricted by their deficient mechanical properties. Here, this work leverages the design freedom offered by additive manufacturing and a novel interlocking pattern to for the first time fabricate a dual‐faced chain mail structure consisting of 3D re‐entrant unit cells. The flexible structured fabric demonstrates high specific energy absorption and specific strength of up to 1530 J kg−1 and 5900 Nm kg−1, respectively, together with an excellent recovery ratio of ≈80%, thereby overcoming the strength–recoverability trade‐off. The designed dual‐faced structured fabric compares favorably against a wide range of materials proposed for wearable applications, attributed to the synergetic strengthening of the energy‐absorbing re‐entrant unit cells and their unique topological interlocking. This work advocates the combined design of energy‐absorbing unit cells and their interlocking to extend the application prospects of fabric‐based materials to shape‐adaptive protection.
Tailoring the Interface with a Multifunctional Ligand for Highly Efficient and Stable FAPbI3 Perovskite Solar Cells and Modules
Authors: Li Fuqiang , Huang Xiaofeng , Ma Chaoqun , Xue Junpeng , Li Ying , Kim Danbi , Yang Hyun‐Seock , Zhang Yuanyuan , Lee Bo Ram , Kim Junghwan , Wu Binghui , Park Sung Heum 
Published: May 2023

AbstractPerovskite solar cells (PeSCs) using FAPbI3 perovskite films often exhibit unfavorable phase transitions and defect‐induced nonradiative interfacial recombination, resulting in considerable energy loss and impairing the performance of PeSCs in terms of efficiency, stability, and hysteresis. In this work, a facile interface engineering strategy to control the surface structure and energy‐level alignment of perovskite films by tailoring the interface between the FAPbI3 film and hole‐transporting layer using 4‐hydroxypicolinic acid (4HPA) is reported. According to density functional theory studies, 4HPA has prominent electron delocalization distribution properties that enable it to anchor to the perovskite film surface and facilitate charge transfer at the interface. By enabling multiple bonding interactions with the perovskite layer, including hydrogen bonds, PbO, and PbN dative bonds, 4HPA passivation significantly reduces the trap density and efficiently suppresses nonradiative recombination. The obtained perovskite films exhibit superior optoelectronic properties with improved crystallinity, pure α‐phase FAPbI3, and favorable energy band bending. Following this strategy, 4HPA post‐treatment PeSCs achieve a champion power conversion efficiency of 23.28% in 0.12 cm2 cells and 19.26% in 36 cm2 modules with excellent environmental and thermal stabilities.
In Situ Constructed 3D Lithium Anodes for Long‐Cycling All‐Solid‐State Batteries
Authors: Duan Hui , Wang Changhong , Yu Ruizhi , Li Weihan , Fu Jiamin , Yang Xiaofei , Lin Xiaoting , Zheng Matthew , Li Xiaona , Deng Sixu , Hao Xiaoge , Li Ruying , Wang Jiantao , Huang Huan , Sun Xueliang 
Published: May 2023

AbstractConstructing a 3D lithium metal anode has been demonstrated to be the most effective strategy to address its dendrite issue in liquid batteries. However, this promising approach has proved challenging to inherit in all‐solid‐state Li metal batteries (ASSLMBs) because of the rigidity of inorganic solid electrolytes (SEs), which constrains interfacial solid–solid ionic contact. Herein, a 3D Li anode is in situ constructed for ASSLMBs by spontaneous chemical reactions between halide SEs and Li metal. The in situ formed Li–Al alloys and well‐maintained sulfide SEs inside the 3D structure serve as continuous electron and Li+ transport pathways and facilitate the homogenous distribution of charge carriers. The lithiophilic Li alloy can regulate Li deposition behavior and enable uniform Li nucleation and deposition. Both the Li||Li symmetric and full batteries exhibit good electrochemical performance at high current density. This work provides a universal strategy and new insight perspective to construct 3D Li for high‐performance ASSLMBs.
Incomplete partitioning of pollinators byLinum suffruticosumand its coflowering congeners
Authors: Pérez‐Barrales Rocío , Armbruster W. Scott 
Published: May 2023

AbstractPremiseLinum suffruticosumshows variations in pollinator fit, pollen pickup, and local pollinators that predict pollen deposition rates. The species often coflowers with otherLinumspecies using the same pollinators. We investigated whetherL. suffruticosumtrait variation could be explained by local patterns of pollinator sharing and associated evolution to reduce interspecific pollen transfer.MethodsPollinator observations were made in different localities (single species, coflowering with other congeners). Floral traits were measured to detect differences across populations and from coflowering species. Reproductive costs were quantified using interspecific hand pollinations and measures of pollen‐tube formation, combined with observations of pollen arrival on stigmas and pollen‐tube formation after natural pollination in allopatric and sympatric localities.ResultsThe size and identity of the most important pollinator ofL. suffruticosumand whether there was pollinator sharing with coflowering species appeared to explain floral trait variation related to pollinator fit. The morphological overlap of the flowers ofL. suffruticosumwith those of coflowering species varied, depending on coflowering species identity. A post‐pollination incompatibility system maintains reproductive isolation, but conspecific pollen‐tube formation was lower after heterospecific pollination. Under natural pollination at sites of coflowering with congeners, conspecific pollen‐tube formation was lower than at single‐species localities.ConclusionsTrait variation inL. suffruticosumappears to respond to the most important local pollinator. Locally, incomplete pollinator partitioning might cause interspecific pollination, imposing reproductive costs. These reproductive costs may generate selection on floral traits for reduced morphological overlap with coflowering congeners, leading to the evolution of pollination ecotypes.
Introducing Trifluoromethoxyarenes as Halide Surrogates in Mechanochemical Realizations of Ni‐catalyzed Cross‐coupling Reactions
Authors: Mkrtchyan Satenik , Jakubczyk Michał , Budzák Šimon , Benická Barbora , Iaroshenko Viktor O. 
Published: May 2023

AbstractSince the introduction of the TM‐catalyzed cross‐couplings their scope of starting materials has been growing systematically, allowing for more flexible design of synthetic routes with the coupling as a key step, particularly late‐stage modifications. Herein we present the use of trifluoromethoxyarenes as halide surrogates in Ni‐catalyzed couplings with arylboronic acids, potassium aryl trifluoroborates and aryl trialkoxysilanes, under mechanochemical conditions. In total, 22 biphenyl structures were obtained in good yields. Successful gram scale experiments confirm the usefulness of our protocol. The developed transformations’ mechanism was discussed in relation to the experimental and literature data and DFT calculations. The unusual reactivity of potassium benzoyltrifluoroborates was uncovered. 6 examples of resulting benzophenones were obtained in very good yields and the protocol was extended to gram scale. The described methods present a powerful tool when combined with the recent advances in organofluorine chemistry.
Asymmetric Hydrogenation of Imines Using NHC‐Phosphine Iridium Complexes
Authors: He Lei , Peters Bram B. C. , Quan Xu , Zhao Shaohu , Huang Tianrui , Ren Shuting , Andersson Pher G. , Zhou Taigang 
Published: May 2023

Abstractα‐Chiral amines represent a frequently observed functional group in pharmaceuticals. Here, the synthesis of such motifs (up to 91% ee) is described by asymmetric hydrogenation of imines catalyzed by NHC,P‐iridium complexes. The hydrogenation proceeded smoothly, even under balloon pressure of hydrogen atmosphere. Mechanistic experiments indicated that the reduction most likely advances by a combination of direct hydrogenation and a hydrogen transfer process using either H2 or iPrOH as the reductant, respectively.
Darobactin B Stabilises a Lateral‐Closed Conformation of the BAM Complex in E. coli Cells
Authors: Haysom Samuel F. , Machin Jonathan , Whitehouse James M. , Horne Jim E. , Fenn Katherine , Ma Yue , El Mkami Hassane , Böhringer Nils , Schäberle Till F. , Ranson Neil A. , Radford Sheena E. , Pliotas Christos 
Published: May 2023

AbstractThe β‐barrel assembly machinery (BAM complex) is essential for outer membrane protein (OMP) folding in Gram‐negative bacteria, and represents a promising antimicrobial target. Several conformational states of BAM have been reported, but all have been obtained under conditions which lack the unique features and complexity of the outer membrane (OM). Here, we use Pulsed Electron‐Electron Double Resonance (PELDOR, or DEER) spectroscopy distance measurements to interrogate the conformational ensemble of the BAM complex in E. coli cells. We show that BAM adopts a broad ensemble of conformations in the OM, while in the presence of the antibiotic darobactin B (DAR‐B), BAM′s conformational equilibrium shifts to a restricted ensemble consistent with the lateral closed state. Our in‐cell PELDOR findings are supported by new cryoEM structures of BAM in the presence and absence of DAR‐B. This work demonstrates the utility of PELDOR to map conformational changes in BAM within its native cellular environment.
Synthesestrategien für die Funktionalisierung homogener Katalysatoren zur Verbesserung ihrer Abtrennbarkeit
Authors: Diekamp Justus , Seidensticker Thomas 
Published: May 2023

AbstractDas Recycling von homogenen Katalysatoren unter Beibehaltung einphasiger Bedingungen ist eine ständige Herausforderung, der sich viele Reaktionen stellen müssen, wenn sie den Schritt in die industrielle Anwendung schaffen sollen. Zwar gibt es eine Fülle von verschiedenen synthetischen Ansätzen für bessere, wiederverwendbare homogene Katalysatoren, doch der Literatur fehlt bisher ein knapper Überblick über die verschiedenen Katalysatormodifikationen. Dieser Übersichtsbeitrag soll diese Lücke schließen, indem die bestehenden Synthesewege zu polaren, unpolaren, fluorhaltigen und molekulargewichtsvergrößerten Katalysatoren zusammengefasst und die jeweiligen Syntheserouten mit Schwerpunkt auf modularen und Late‐Stage‐Ansätzen untersucht werden. Darüber hinaus zeigen wir das Potenzial für eine allgemein anwendbare Bibliothek auf, die es ermöglicht, Katalysatoren durch einfache Modifikationen auf die jeweils gewünschte Recyclingstrategie abzustimmen.
Ion‐Selective Electrodes With Sensing Membranes Covalently Attached to Both the Inert Polymer Substrate and Conductive Carbon Contact
Authors: Choi Kwangrok R. , Troudt Blair K. , Bühlmann Philippe 
Published: May 2023

AbstractThe use of solid‐contact ion‐selective electrodes (ISEs) is of interest to many clinical, environmental, and industrial applications. However, upon extended exposure to samples and under thermal and mechanical stress, adhesion between these membranes and underlying substrates often weakens gradually. Eventually, this results in the formation of a water layer at the interface to the underlying electron conductor and in delamination of the membrane from the electrode body, both major limitations to long‐term monitoring. To prevent these problems without increasing the complexity of design with a mechanical attachment, we use photo‐induced graft polymerization to simultaneously attach ionophore‐doped crosslinked poly(decyl methacrylate) sensing membranes covalently both to a high surface area carbon as ion‐to‐electron transducer and to inert polymeric electrode body materials (i.e., polypropylene and poly(ethylene‐co‐tetrafluoroethylene)). The sensors provide high reproducibility (standard deviation of E0 of 0.2 mV), long‐term stability (potential drift 7 μV h−1 over 260 h), and resistance to sterilization in an autoclave (121 °C, 2.0 atm for 30 min). For this work, a covalently attached H+ selective ionophore was used to prepare pH sensors with advantages over conventional pH glass electrodes, but similar use of other ionophores makes this approach suitable to the fabrication of ISEs for a variety of analytes.
Nanostructure‐Driven Indocyanine Green Dimerization Generates Ultra‐Stable Phototheranostics Nanoparticles
Authors: Kwon Nahyun , Jasinevicius Gabriel O. , Kassab Giulia , Ding Lili , Bu Jiachuan , Martinelli Letícia P. , Ferreira Vinicius G. , Dhaliwal Alexander , Chan Harley H. L. , Mo Yulin , Bagnato Vanderlei S. , Kurachi Cristina , Chen Juan , Zheng Gang , Buzzá Hilde H. 
Published: May 2023

AbstractIndocyanine green (ICG) is the only near‐infrared (NIR) dye approved for clinical use. Despite its versatility in photonic applications and potential for photothermal therapy, its photobleaching hinders its application. Here we discovered a nanostructure of dimeric ICG (Nano‐dICG) generated by using ICG to stabilize nanoemulsions, after which ICG enabled complete dimerization on the nanoemulsion shell, followed by J‐aggregation of ICG‐dimer, resulting in a narrow, red‐shifted (780 nm→894 nm) and intense (≈2‐fold) absorbance. Compared to ICG, Nano‐dICG demonstrated superior photothermal conversion (2‐fold higher), significantly reduced photodegradation (−9.6 % vs. −46.3 %), and undiminished photothermal effect (7 vs. 2 cycles) under repeated irradiations, in addition to excellent colloidal and structural stabilities. Following intravenous injection, Nano‐dICG enabled real‐time tracking of its delivery to mouse tumors within 24 h by photoacoustic imaging at NIR wavelength (890 nm) distinct from the endogenous signal to guide effective photothermal therapy. The unprecedented finding of nanostructure‐driven ICG dimerization leads to an ultra‐stable phototheranostic platform.
Catalytic Diastereo‐ and Enantioselective Cyclopropanation of gem‐Dihaloalkanes and Terminal Olefins
Authors: Liu Hou‐Lu , Wang Xinyu , Gao Ke , Wang Zhaobin 
Published: May 2023

AbstractChiral cyclopropane derivatives are essential in synthetic chemistry and drug discovery. Their synthesis commonly relies on asymmetric cyclopropanation of diazo compounds, potentially explosive and needing stabilizing substituents. Thus, asymmetric catalytic transformations of non‐stabilized carbenes or carbenoids remain a formidable challenge. Herein, we report the unprecedented chromium‐catalyzed asymmetric cyclopropanation of readily available gem‐dihaloalkanes and terminal olefins. Distinct from previous approaches, gem‐dihaloalkanes serve as suitable precursors for non‐stabilized carbenes or carbenoids, furnishing various functionalized chiral cyclopropanes. Mechanistic studies, including radical trapping, non‐linear effect, and UV/Vis spectroscopy, provide insights into the catalytic process, featuring radical‐polar crossover.
Deletions of DNA in cancer and their possible uses for therapy
Authors: Varshavsky Alexander , Lewis Kim , Chen Shun‐Jia 
Published: May 2023

AbstractDespite advances in treatments over the last decades, a uniformly reliable and free of side effects therapy of human cancers remains to be achieved. During chromosome replication, a premature halt of two converging DNA replication forks would cause incomplete replication and a cytotoxic chromosome nondisjunction during mitosis. In contrast to normal cells, most cancer cells bear numerous DNA deletions. A homozygous deletion permanently marks a cell and its descendants. Here, we propose an approach to cancer therapy in which a pair of sequence‐specific roadblocks is placed solely at two cancer‐confined deletion sites that are located ahead of two converging replication forks. We describe this method, termed “replication blocks specific for deletions” (RBSD), and another deletions‐based approach as well. RBSD can be expanded by placing pairs of replication roadblocks on several different chromosomes. The resulting simultaneous nondisjunctions of these chromosomes in cancer cells would further increase the cancer‐specific toxicity of RBSD.
Neuroimaging findings in DYT1 dystonia and the pathophysiological implication: A systematic review
Authors: Taiwo Funmilola T. , Adebayo Philip B. 
Published: May 2023

AbstractBackgroundPrimary generalized dystonia due to the DYT1 gene is an autosomal dominant disorder caused by a GAG deletion on chromosome 9q34. It is a well‐defined, genetically proven, isolated dystonia syndrome. However, its pathophysiology remains unclear.ObjectivesThis study was aimed at profiling the functional neuroimaging findings in DYT1 dystonia and harmonizing the pathophysiological implications for DYT1 dystonia from the standpoint of different neuroimaging techniques.MethodsA systematic review was conducted using identified studies published in English from Medline, PsycINFO, Embase, CINAHL, and the Cochrane Database of Systematic Reviews (CDSR), between 1985 and December 2019 (PROSPERO protocol CRD42018111211).ResultsAll DYT1 gene carriers irrespective of clinical penetrance have reduced striatal GABA, dopamine receptors and increased metabolic activity in the lentiform nucleus, supplementary motor area, and cerebellum in addition to an abnormal cerebellothalamocortical pathway. Nonmanifesting carriers on the other hand have a disruption of the distal (thalamocortical) segment and have larger putaminal volumes than manifesting carriers and healthy controls. Activation of the midbrain, thalamus, and sensorimotor cortex was only found in the manifesting carriers.ConclusionsTherefore, we propose that DYT1 dystonia is a cerebellostriatothalamocortical network disorder affecting either the structure or function of the different structures or nodes in the network.
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates
Authors: São Pedro Mariana N. , Eppink Michel H. M. , Ottens Marcel 
Published: May 2023

AbstractThe lack of process analytical technologies able to provide real‐time information and process control over a biopharmaceutical process has long impaired the transition to continuous biomanufacturing. For the monoclonal antibody (mAb) production, aggregate formation is a major critical quality attribute (CQA) with several known process parameters (i.e., protein concentration and agitation) influencing this phenomenon. The development of a real‐time tool to monitor aggregate formation is then crucial to gain control and achieve a continuous processing. Due to an inherent short operation time, miniaturized biosensors placed after each step can be a powerful solution. In this work, the development of a fluorescent dye‐based microfluidic sensor for fast at‐line PAT is described, using fluorescent dyes to examine possible mAb size differences. A zigzag microchannel, which provides 90% of mixing efficiency under 30 s, coupled to an UV–Vis detector, and using four FDs, was studied and validated. With different generated mAb aggregation samples, the FDs Bis‐ANS and CCVJ were able to robustly detect from, at least, 2.5% to 10% of aggregation. The proposed FD‐based micromixer is then ultimately implemented and validated in a lab‐scale purification system, demonstrating the potential of a miniaturized biosensor to speed up CQAs measurement in a continuous process.