bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic Predictions of Genetic Variances and Correlations Among Traits: Empirical Validation within the Soybean Nested Association Mapping Population
Authors: Lorenz Aaron , Wartha Cleiton Antonio 
Published: September 2023

Abstract

Parental selection is perhaps the most critical decision a breeder makes, establishing the foundation of the entire program for years to come. Cross selection based on predicted mean and genetic variance can be further expanded to multiple-trait improvement by predicting the genetic correlation (r_G) between pairs of traits. Genetic correlation among traits is caused by pleiotropy or linkage. Unfavorable correlations due to linkage can be disrupted by successive recombination events. Increased multi-trait genetic gain could be obtained by selecting parental combinations with a desirable mean, greater genetic variance, and more favorable r_G. The accuracy of predicting r_G of soybean breeding crosses using genome-wide markers remains unexplored. Our objective was to empirically measure the ability to predict the family mean, genetic variance, superior progeny mean and genetic correlation. Thirty-nine validation families that differed in their estimated mean and variance were evaluated for days to maturity, lodging, oil, plant height, protein, seed size, and seed yield in multi-environment trials. Training population composition followed a leave-one-family-out cross-validation scheme, with the validation family genetic parameters predicted using the remaining families as the training set. The predictive abilities for family mean and superior progeny mean were significant for all traits while predictive ability of genetic variance was significant for four traits. We were able to validate significant predictive abilities of r_G for 17 out of 21 (80%) pairwise trait combinations (P < 0.05; bootstrapping). The findings from this study support the use of genome-wide marker effects for predicting r_G in soybean biparental crosses.
Clinical classification of far lateral lumbar disc herniation under 3D-DESS imaging technology: guidance for PTED operation
Authors: Zheng Junwen , Liu Wei , Zhu Qing , Li Guangsheng , Wu Gang , Li Haijun 
Published: September 2023

Abstract

Backgroud:Many far lateral lumbar disc herniation (FLLDH) classifications utilize image technology to describe the size, shape, and location of disc herniation. However, these approaches are fail in illustrating the spatial relationship between disc herniation and nerve roots. Their utility in offering guidance for the clinical execution of percutaneous transforaminal endoscopic discectomy (PTED) is limited and isgenerally more suited for open surgery. The aim of this study was to propose a clinical classification for FLLDH based on three-dimensional double-echo steady-state (3D-DESS) imaging technology and to provide guidance with precise cannula placement and surgical operation for PTED.
Methods: A retrospective analysis was conducted on the images of 178 FLLDH patients diagnosed by 3D-DESS. Based on the positional relationship between nerve roots and disc herniation, FLLDH patients were classified into 3 types: type I, axillary tennis type; type II, axillary elevated type; and type III, shoulder type. Among these, 68 patients underwent PTED treatment. The clinical results were assessed using the Visual Analog Scale (VAS) leg pain score, the Oswestry Disability Index (ODI) score, and the modified Macnab criteria.
Results: The average age of the patients was 61.29 ± 11.60 years, with a male/female ratio of 42:26. According to the 3D-DESS classification, there were I=25, II=40 and III=3. The average operation time was 66.91 ± 18.87 minutes, the average follow-up was 24.27±4.66 months, and the recurrence rate was 6%. The VAS leg pain score improved from 7.63 ± 0.91 to 0.69 ± 1.37. The ODI score improved from 51.27 ± 9.09 to 3.87 ± 5.75. The modified Macnab criteria results showed that 35 patients (51%) were excellent, 25 patients (37%) were good, 4 patients (6%) were fair and 4 patients (6%) were poor. No complications, such as infection or nerve or vascular injury, occurred in the patients.
Conclusions: Using 3D-DESS to classify FLLDH patients can provide clinical guidance with precise puncture and treatment for PTED. It helps operators remove disc herniation, decompress nerve roots, shorten operation time, and improve the success rate of PTED.
Caloric restriction promotes beta cell longevity and delays aging and senescence signatures.
Authors: Drigo Rafael Arrojo e , Santos Cristiane dos , Shrestha Shristi , Cottam Matthew , Perkins Guy , Lev-Ram Varda , Roy Birbickram , Acree Christopher , Kim Keun-Young , Deerinck Thomas , Cutler Melanie , Dean Danielle , Cartailler Jean-Philippe , MacDonald Patrick , Hetzer Martin , Ellisman Mark 
Published: September 2023

Abstract

Caloric restriction (CR) extends organismal lifespan and health span by improving glucose homeostasis mechanisms. How CR affects organellar structure and function of pancreatic beta cells over the lifetime of the animal remains unknown. Here, we used single nucleus transcriptomics to show that CR increases the expression of genes for beta cell identity, protein processing, and organelle homeostasis. Gene regulatory network analysis link this transcriptional phenotype to transcription factors involved in beta cell identity (Mafa) and homeostasis (Atf6). Imaging metabolomics further demonstrates that CR beta cells are more energetically competent. In fact, high-resolution light and electron microscopy indicates that CR reduces beta cell mitophagy and increases mitochondria mass, increasing mitochondrial ATP generation. Finally, we show that long-term CR delays the onset of beta cell aging and senescence to promote longevity by reducing beta cell turnover. Therefore, CR could be a feasible approach to preserve compromised beta cells during aging and diabetes.
RETRACTED: Investigations on Electric Discharge Machining Performances of AA8011-10wt.% TiO2 Composite using MCDM Approach
Authors: S Balaji , Ravi Samraj , Sivasamy Alagarsamy , Singh Ravindra Pratap , Srini Yandra , Kannan Sathish , Usmani Yusuf Siraj 
Published: September 2023

Abstract

The authors have requested that this preprint be removed from Research Square.
Profiles of free surfaces in revolved containers under microgravity
Authors: Chen Shuyang , Duan Li , Li Wen , Chen Shangtong , Kang Qi 
Published: September 2023

Abstract
Nowadays a propellant residual gauging method based on the thermal response of the tanks’ wall is developed. And the liquid distribution and meniscus height have great effects on the thermal response. Profiles of liquid free surfaces in revolved containers under microgravity are studied through theoretical analysis and numerical simulation in this paper. The analytical formula for the static profile of the liquid surface in the spherical tank is established. It shows that the profile is a section of a circle cut off by the tank wall. For given the geometry of the tank, liquid volume and contact angle, the profile of the free surfaces under microgravity can be obtained by using the Shooting method based on the theoretical model. Numerical simulation is carried out with the Volume of Fluid method, and it is verified that the static profiles at different contact angles and liquid filling rates fit the theoretical descriptions. It is concluded that the meniscus height increases slowly as the filling rate increases, and the smaller the contact angle, the more obvious this trend. Then the theory is extended to the tanks of arbitrary shapes, and the critical position of the profile is derived. Below the critical position the propellant may accumulate in some corners or pits, which makes it unable to be fully utilized. The critical position is related to the shape of the tank and the contact angle. This research is of great value for the prediction of the static profiles of liquid surfaces in tanks and the propellant residual gauging.
Kidney biopsy findings in children with diabetes mellitus
Authors: Weerasooriya Lasanthi , Howie Alexander J , Wakeman Matthew P , Cavanagh Susan , Milford David V 
Published: September 2023

Abstract

Background
Diabetic glomerulopathy may begin in childhood, but clinical kidney disease ascribable to this is uncommon in children with type 1 (insulin dependent) diabetes mellitus.
Methods
We reviewed our experience of kidney biopsies in children with type 1 diabetes mellitus.
Results
Between 1995 and 2022, there were biopsies in 17 children, with various clinical indications for biopsy, making this the largest series of biopsies in diabetic children with clinical kidney abnormalities. Four biopsies showed diabetic glomerulopathy, three showed diabetic glomerulopathy and IgA nephropathy, and ten showed a variety of conditions other than diabetic glomerulopathy: minimal change nephropathy (2), membranous nephropathy (2), thin glomerular basement membrane disease (2), non-glomerular chronic damage in Wolcott-Rallison syndrome (2), acute vasculitic glomerulonephritis (1), and IgA nephropathy (1). Clinical clues of something other than diabetic glomerulopathy included acute kidney injury, microscopic haematuria or chronic kidney impairment with little or no proteinuria, and the nephrotic syndrome after a short duration of diabetes. In addition, there were biopsies in two children with cystic fibrosis that showed changes identical with diabetic glomerulopathy, although the children were not shown to have diabetes mellitus.
Conclusions
We confirm that changes better known in adults with either type 1 or type 2 diabetes mellitus can occur in children with type 1: overt diabetic glomerulopathy either on its own or combined with other conditions, and kidney disorders other than diabetic glomerulopathy. Also, changes resembling diabetic glomerulopathy can be found in children with cystic fibrosis who are not diagnosed to have diabetes mellitus.
Pythium Survey Europe provides deep insights
into the genetic and structural diversity of Pythium communities and their disease potential in Europe
Authors: Cai Daguang , Boie Wilken , Schemmel Markus , Ye Wanzhi , Hasler Mario , Goll Melanie , Verreet Joseph 
Published: September 2023

Abstract

Pythium spp. poses a serious threat to agricultural production worldwide, but its severity is often neglected because little knowledge about it is available. Using an ITS amplicon metagenomics approach, we investigated the occurrence, abundance, and diversity of Pythium spp. in 127 corn fields of 11 European countries over three years. As a result, we identified 69 Pythium species, with up to 19 species in a single soil sample. We identified also highly prevalent and distributed species in most countries, with significant genetic diversity and varying disease potential, demonstrating a dynamic sharpening process of Pythium communities in Europe corn fields. Infection experiments with isolated isolates indicate that Pythium species differ strongly in their infection progression and disease potential, where an effective interference with plant hormone networks to suppress JA (jasmonate)-mediated defenses seems to be a crucial part of the virulence mechanism of pathogenic Pythium species. This study provides for the first time a valuable data set to gain deep insights into the structural and genetic diversity of Pythium communities in European corn fields and to better understand plant-Pythium interactions and, in particular, the evolution of virulence and pathogenicity, thus facilitating the development of an effective strategy to cope with this pathogen.
COVID-19-related Health Outcomes during Delivery Hospitalization During Pre-delta, Delta, and Omicron Periods
Authors: Carlson Jeffrey , Simeone Regina , Ellington Sascha , Galang Romeo , DeSisto Carla , Fleming-Dutra Katherine , Riley Laura , Meaney-Delman Dana , Tong Van 
Published: September 2023

Abstract

Background
SARS-CoV-2 infection during pregnancy has been associated with several adverse maternal health and pregnancy outcomes; however, few studies have included pregnancies occurring during the time period of Omicron variant predominance (January 2022–present) in the evaluation of pregnancy outcomes. We examined the relationship between COVID-19 diagnosis at delivery and adverse maternal and pregnancy outcomes for each variant period, with a focus on Omicron.
Methods
Data on delivery hospitalizations from the Premier Healthcare Database during February 2020 –August 2023 were analyzed as a cross-sectional observational study. Adjusted prevalence ratios (aPR) were calculated to compare the risks of adverse outcomes between women with and without a COVID-19 diagnosis at the time of delivery for each variant period.
Results
Among 2,990,973 women with a delivery hospitalization, 1.9% (n = 56,618) had a COVID-19 diagnosis noted on discharge, including 26,053 during the Omicron period. In adjusted models, COVID-19 during the Omicron period was associated with a significant increased risk for maternal sepsis (COVID-19: 0.4% vs No COVID-19: 0.1%) (aPR: 3.32 [95%CI: 2.70, 4.08]), acute respiratory distress syndrome (0.6% vs 0.1%) (6.19 [5.26, 7.29]), shock (0.2% vs 0.1%) (2.14 [1.62, 2.84]), renal failure (0.5% vs 0.2%) (2.08 [1.73, 2.49]), ICU admission (2.7% vs 1.7%) (1.64 [1.53, 1.77]), mechanical ventilation (0.3% vs 0.1%) (3.15 [2.52, 3.93]), in-hospital death (0.0% vs 0.0%)(5.00 [2.30, 10.90]), stillbirth (0.7% vs 0.6%) (1.17 [1.01, 1.36]), and preterm birth (12.3% vs 9.6%) (1.34 [1.27, 1.42]).
Conclusions
During the Omicron variant period, risks of adverse outcomes associated with COVID-19 diagnosis at delivery remain elevated, despite the possibility of some level of immunity due to previous SARS-COV-2 infection or vaccination. It remains important for pregnant people, and those planning pregnancy, to stay up to date with COVID-19 vaccination to reduce the risk of adverse outcomes.
Caspase-11 mediated inflammasome activation in macrophages by systemic infection of A. actinomycetemcomitans exacerbates arthritis.
Authors: Okano Tokuju , Ashida Hiroshi , Komatsu Noriko , Tsukasaki Masayuki , Iida Tamako , Iwasawa Marie , Takahashi Yuto , Takeuchi Yasuo , Iwata Takanori , Sasai Miwa , Yamamoto Masahiro , Takayanagi Hiroshi , Suzuki Toshihiko 
Published: September 2023

Abstract

Clinical studies have shown that Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is associated with aggressive periodontitis and can potentially trigger or exacerbate rheumatoid arthritis (RA). However, the mechanism is poorly understood. Here, we show that systemic infection with A. actinomycetemcomitans triggers the progression of arthritis in mice anti-collagen antibody-induced arthritis (CAIA) model following IL-1β secretion and cell infiltration in paws in a manner that is dependent on caspase-11-mediated inflammasome activation in macrophages. The administration of polymyxin B (PMB), chloroquine, and anti-CD11b antibody suppressed inflammasome activation in macrophages and arthritis in mice, suggesting that the recognition of lipopolysaccharide (LPS) in the cytosol after bacterial degradation by lysosomes and invasion via CD11b are needed to trigger arthritis following inflammasome activation in macrophages. These data reveal that the inhibition of caspase-11-mediated inflammasome activation potentiates the aggravation of RA induced by infection with A. actinomycetemcomitans. This work highlights how RA can be progressed by inflammasome activation as a result of periodontitis-associated bacterial infection and discusses the mechanism of inflammasome activation in response to infection with A. actinomycetemcomitans.
Management of pain in cancer patients— Lessons from practices during the COVID-19: A qualitative study of cancer care providers’ perspectives
Authors: Cornall Georgina , Zhao Emma , Luckett Tim , Erciyas Ertugrul , Monck David , Glare Paul , Wang Andy , Lee Yi-Ching 
Published: September 2023

Abstract
Background
The ongoing COVID-19 pandemic has impacted health systems globally and affected managing many chronic conditions, including cancer. This study aimed to explore the perceptions of multi-disciplinary cancer care providers on how cancer pain management was affected by the COVID-19 pandemic.
Methods
People were eligible if they were cancer care providers of any specialty and discipline from two tertiary hospitals in Australia. Data were collected using semi-structured interviews exploring their perceptions of how COVID-19 has affected cancer pain management. Thematic analysis of interview transcripts used an integrated approach that started with inductive coding before coding deductively against a framework for behaviour change called the COM-B Model which proposes that ‘capability’, ‘motivation’ and ‘opportunity’ are requisites for any behaviour.
Results
Twenty-three providers participated. Five themes were developed and interpreted from the analysis of data, namely: “Telehealth enables remote access to cancer pain management but also created a digital divide”, “Access to cancer pain management in the community is compromised due to the pandemic”, “COVID-19 negatively impacts hospital resource allocation”, “Patients were required to trade off cancer pain management against other health priorities” and “Hospital restrictions result in decreased social and psychological support for patients with cancer pain”.
Conclusions
The landscape of the cancer pain management health system in Australia underwent substantial shifts during the COVID-19 pandemic, with lasting impacts. This study identifies unique challenges faced by patients with cancer that were not well studied amongst ample literature on pandemic pain management.
Impaired binding affinity of YTHDC1 with METTL3/METTL14 results in R-loop accumulation in myelodysplastic neoplasms with DDX41 mutation
Authors: kim hongtae , Hwang Won Chan , Park Kibeom , Park Silvia , Cheon Na Young , Lee Ja Yil , Lee Jong-Mi , Ju Min Kyung , Lee Joo Rak , Kwon Yong-Rim , Jo Woo-Lam , Kim Myungshin , Kim Yoo-Jin 
Published: September 2023

Abstract

DEAD box helicase 41 (DDX41) mutations are the most prevalent predisposition to familial myelodysplastic syndrome (MDS). However, the precise roles of these variants in the pathogenesis of MDS have yet to be elucidated. Here, we discovered a novel mechanism by which DDX41 contributes to R-loop-induced DNA damage responses (DDR) in cooperation with the m6A-METTL complex (MAC) and YTHDC1 usingDDX41knockout (KO) andDDX41knock-in (KI, R525H, Y259C) cell lines as well as primary samples from MDS patients. Compared to wild type (WT),DDX41KO and KI led to increased levels of m6A RNA methylated R-loop and increased DNA damage. Interestingly, we found that DDX41 regulates m6A/R-loop levels by interacting with MAC components. Further, DDX41 promoted the recruitment of YTHDC1 to R-loops by promoting the binding between METTL3 and YTHDC1, which was dysregulated inDDX41-deficient cells. Collectively, we demonstrated that DDX41 plays a key role in the physiological control of R-loops in cooperation with MAC and YTHDC1. These findings provide novel insights into how defects in DDX41 influence MDS pathogenesis and suggest potential therapeutic targets for the treatment of MDS.
Opportunities to address employee voice in health care providers: A scoping review
Authors: Kepplinger Anja , Braun Alexander , Fringer André , Roes Martina 
Published: September 2023

Abstract

Background: Employees’ decision to speak up or to stay silent can have implications for health care providers, employees and people who need care. As a result, a shift is needed from blindly following guidelines to implementing a sustainable proactive organizational culture in which employees can evaluate their work environment and take advantage of growth opportunities. Employee voice refers to all organizational structures, mechanisms or practices in which employees participate and through which they try to influence their work and the performance of their organization.
Aims and objective: This scoping review aims to provide an overview of the literature and research on opportunities to address employee voice in health care providers. The review will provide insights into what research already exists in health care related to employee voice and discuss the described opportunities in an attempt to improve employee voice. The results will also form the basis of the interview guide for a case study, ADVICE.
Methods: The search was conducted from September 2022 to April 2023 in the following databases: MEDLINE via PubMed, CINHAL via EBSCO, Scopus via Elsevier, Wiley/Web of Science and Cochrane Library. The search results were imported into the COVIDENCE program and screened by two researchers separately. We used the following search terms: employee voice, employee participation, employee engagement, speaking up, opportunities and health-care provider. Furthermore, the search terms and their synonyms are linked to search strings using Boolean operators dependent on the databases, as well as Mesh terms. The review followed the PRISMA-ScR guidelines.
Results: We identified 951 studies in five databases and via citation tracking. After we removed 102 duplicates and screening 839 titles and abstracts, 23 full texts were assessed. According to our inclusion and exclusion criteria, we included 10 studies.
Conclusions: Opportunities to address employee voice are not effective on their own, they need to be seen in a wider context. Employees need to learn how to raise their concerns, and managers need to learn how to empower employees to speak up and use the improvements to change the work environment and improve patient outcomes.