bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircANKRD12/circTIMMDC1 act as miR-181b-5p sponge to synergistically regulate neural cell migration via the PROX1-NOTCH1-HES1 axis in Hirschsprung's disease
Authors: Fu Runxi , Wang Chen , Zhao Yuling , Liu Xiaowei , Zhou Ying , Gu Beilin , Pan Weihua , Wang Yang , Cai Wei 
Published: January 2023

AbstractBackground:In recent years, emerging researches have shown that circular RNA play critical regulatory roles in a variety of diseases. However, the biogenesis, regulation, function and mechanism of circRNAs in Hirschsprung's disease remain largely unknown.Methods:qRT-PCR was used to determine the expression of circANKRD12/circTIMMDC1 in HSCR tissues. Transwell and wound healing assay were conducted to assess the role of circANKRD12/circTIMMDC1 in neural cell migration. Dual-luciferase reporter assay, RIP assay and RNA pull-down assay were performed to evaluate the direct interaction between circANKRD12/circTIMMDC1 and miR-181b-5p and alleviate suppression on target PROX1 expression.Results:The expression of circANKRD12/circTIMMDC1 and PROX1 were down-regulated in HSCR tissues compared with control tissues, while the miR-181b-5p, NOTCH1, HES1 expression were up-regulated. Knockdown of circANKRD12 and circTIMMDC1 synergically inhibited the migration of human neural cells, whereas overexpression of circANKRD12 and circTIMMDC1 had the opposite effects. Transfection of miR-181b-5p inhibitor or mimics reversed the effects of circANKRD12/circTIMMDC1 on cell migration. We demonstrated that circANKRD12/circTIMMDC1 acted as “molecular sponge” of miR-181b-5p to rescue the repressive effect of miR-181b-5p on its target PROX1, and suppressed neural cell migration through activating NOTCH1-HES1 signaling pathway.Conclusions:Our research reveals a novel negative regulatory loop circANKRD12/circTIMMDC1-miR-181b-5p-PROX1-NOTCH1-HES1, in the pathogenesis of HSCR providing an exploitable biomarker and therapeutic targets for HSCR.
Passty framework of Chambolle-Pock for Fast and High accuracy CT reconstruction
Authors: Kim Yongchae , Yu Jaesok 
Published: January 2023

Abstract
To realize low-dose CT, many iterative reconstruction methods have been proposed, but many iterations and high computational complexity are required for each scan. It is necessary to reduce the number of iterations while effectively suppressing artifacts and noise caused by reducing radiation dose for practical use. This study aims to accelerate the algorithm by improving the Chambolle-Pock (CP) algorithm, the latest first-order primal-dual. First, we introduce the Passty framework into the CP algorithm. The proposed CP becomes a row-action type algorithm, enabling the incorporation of ordered subsets to accelerate the algorithm further. Second, to step up with the latest trends, the regularization term (nonlocal TV) is designed with a combined model of the first and second-order derivatives to preserve smooth intensity changes. Third, we extend the proposed CP to the L1 and Huber data-fidelity terms for more practical application. Most CT reconstruction studies employ the L2 data-fidelity term because of its good convergence. Even if the L1 data-fidelity term has tremendous potential in noise immunity, it has a fatal drawback of poor convergence and is not adopted at present. In the proposed CP, the L1 and Huber data-fidelity terms have excellent image quality and convergence performance than the L2.
Data-driven predictions of complex mixture permeation in polymer membranes
Authors: Lee Young Joo , Chen Lihua , Nistane Janhavi , Jang Hye Youn , Weber Dylan , Scott Joseph , Rangenekar Neel , Marshall Bennett , Li Wenjun , Johnson JR , Bruno Nicholas , Finn M.G. , Ramprasad Rampi , Lively Ryan 
Published: January 2023

Abstract
Membrane-based organic solvent separations are rapidly emerging as a promising class of technologies for enhancing the energy efficiency of existing separation and purification systems. Polymeric membranes have shown promise in the fractionation or splitting of complex mixtures of organic molecules such as crude oil. Determining the separation performance of a polymer membrane when challenged with a complex mixture has thus far occurred in an ad hoc manner, and methods to predict the performance based on mixture composition and polymer chemistry are unavailable thus far. Here, we combine physics-informed machine learning algorithms and mass transport simulations to create an integrated predictive model for the separation of complex mixtures containing up to 400 components via any arbitrary linear polymer membrane. We experimentally demonstrate the effectiveness of the model by predicting the separation of two crude oils within 6-7 % of the measurements. Integration of ML predictors of key sorption and transport properties of molecules with transport simulators constitutes a radical departure from traditional approaches and will open new avenues for the rapid screening of polymer membranes prior to physical experimentation for the separation of complex liquid mixtures.
Identifying Protective Factors for Gender Diverse Adolescents’ Mental Health
Authors: Holt Melissa , Parodi Katharine , Elgar Frank , Vigna Abra , Moore LB , Koenig Brian 
Published: January 2023

Abstract
Few studies have disentangled differences in victimization exposures and mental health symptoms among gender diverse subgroups, nor considered the role of potential protective factors in ameliorating the impact of victimization on gender diverse youths’ mental health. To address this gap, we analyze data (N = 11,264) from a population-based survey of youth in participating school districts in a large Midwestern U.S. county. Relative to cisgender youth with gender conforming expression, transgender youth and cisgender youth with nonconforming gender expression are more likely to experience victimization and severe mental health concerns. Additionally, school-connectedness moderates the association between bias-based harassment and depression for cisgender youth with gender nonconforming expression, and family support/monitoring buffers the association of peer victimization with suicide attempts among transgender youth. Findings highlight the need to better understand factors which may confer protection among gender diverse adolescents, so that in turn appropriate supports across key contexts can be implemented.
IKKα inhibition re-sensitizes acquired adriamycin-resistant triple negative breast cancer cells to chemotherapy-induced apoptosis
Authors: Liao Jian , Qin Qing-hong , Lv Fa-you , Huang Zhen , Lian Bin , Wei Chang-yuan , Mo Qin-guo , Tan Qi-xing 
Published: January 2023

Abstract
IKKα has been shown to be responsible of multiple pro-tumorigenic functions and therapy resistance independent of canonical NF-κB, but its role in acquired chemotherapy resistance in breast cancer remains unclarified. In this study, we obtained pre-treatment biopsy and post-treatment mastectomy specimens from aretrospective cohort of triple-negative breast cancer (TNBC) patients treated with neoadjuvant chemotherapy(NAC) (n = 43). Immunohistochemical methods were used to detect the expression of IKKα before and after NAC, and the relationship between IKKα and the pathologic response to NAC was examined. In addition, we developed a new ADR-resistant MDA-MB-231 cell line(MDA-MB-231/ADR) and analyzed these cells for changes in IKKα expression, the role and mechanisms of the increased IKKα in promoting drug resistance were determined in vitro and in vivo. We demonstrated that the expression of IKKα in residual TNBC tissues after chemotherapy was significantly higher than that before chemotherapy, and was positively correlated with lower pathological reaction. IKKα expression was significantly higher in ADR-resistant TNBC cells than in ADR-sensitive cells, IKKα knockdown results in apoptotic cell death of chemoresistant cells upon drug treatment. Moreover, IKKα knockdown promotes chemotherapeutic drug-induced tumor cell death in an transplanted tumor mouse model. Functionally, we demonstrated that IKKα knockdown significantly upregulated the expression of cleaved caspase 3 and Bax and inhibited the expression of Bcl-2 upon ADR treatment. Our findings highlighted that IKKα exerts an important and previously unknown role in promoting chemoresistance in TNBC, combining IKKα inhibition with chemotherapy may be an effective strategy to improve treatment outcome in chemoresistant TNBC patients.
Peripheral blood white blood cells, monocytes, basophils, aspartate aminotransferase, and prothrombin time ratio after two cycles of treatment were independently associated with efficacy of neoadjuvant chemoimmunotherapy in non-small cell lung cancer
Authors: Hu Xingsheng , Hu Chunhong , Liu Xianling , Liu Chaoyuan , Zhong Ping , Xie Junpeng 
Published: January 2023

Abstract
Neoadjuvant chemoimmunotherapy (NCIO) is a new and effective treatment for cancer, but it is not clear for which patients it is suitable. In our previous study, we did not find any correlation between baseline peripheral blood routine tests and the efficacy of NCIO in non-small cell lung cancer (NSCLC). In this study, we aimed to investigate whether changes in these routine tests after treatment were associated with efficacy of NCIO in NSCLC. We retrospectively collected clinical data of patients with NSCLC who received NCIO followed by surgery from January 2019 to July 2022 at our institution.We included 95 patients with stage IIB-IIIC NSCLC. At week 6 after two cycles of NCIO, the absolute or changed values (or rates) in peripheral blood routine tests were associated with pathological complete response (pCR)/major pathological response (MPR). Changed rate of white blood cell (WBC) count [adjusted odds ratio (OR) = 5.24, P = 0.003] and changed rate of monocyte count (adjusted OR = 4.67, P = 0.017), and absolute prothrombin time ratio at week 6 (adjusted OR = 8.27, P = 0.012) were independently associated with pCR. Changed value of WBC count (adjusted OR = 4.23, P = 0.048), absolute basophil count at week 6 (adjusted OR = 5.05, P = 0.023), changed rate of monocyte count (adjusted OR = 4.37, P = 0.034) and absolute aspartate aminotransferase (AST) level at week 6 (adjusted OR = 15.78, P < 0.001) were independently associated with MPR. Based on the five independent factors, we established easy-to-use clinical models to predict pCR and MPR with area under the curve (AUC) values of 0.785 and 0.881, respectively. Of note, we found change' rate of monocyte count, absolute basophil count, AST and blood magnesium levels at week 6 may be promising biomarkers for NCIO in NSCLC. These peripheral blood routine tests at week 6 after NCIO were better predicted the efficacy of NCIO in NSCLC than at baseline. It is feasible to establish clinical predictive models through these parameters, and their predictive values were consistent with the pathological criteria of pCR and MPR.
COVID-19-Induced Workplace Health and Safety Interventions from Developing Nations: From the Big Event Perspective
Authors: Ansah Edward W. , Sarfo Jacob O. , Amoadu Mustapha , Appiah-Ankomah Emmanuel 
Published: January 2023

Abstract
Background: Big Events like the COVID-19 pandemic have resulted in disruptions of socio-economic activities as well as work life. The disruption of social support systems and economic activities can be detrimental to human health and survival. In such situations, social structures such as workplaces need to be re-structured to effectively support individuals, communities as well protect and promote the wellbeing and safety of workers. The purpose of this study is to identify occupational health and safety (OHS) policies and measures institutions/businesses adopted to protect the health and safety and promote a safe working environment for workers during the pandemic.
Method: The interventions were identified from 21 organizations through direct phone and email conversations, search through organizations’ website publications and press releases.
Results: It was found that most of these organizations instituted policies on the use of personal protective equipment (PPE) and provided significant information about the biological hazards of the pandemic. The organizations also adopted work-from-home strategy for infested and exposed workers and to limit human contact at their workplaces. However, only a few organizations provided psychosocial support such as direct mental health support for COVID-19 victims and their families, reduction in workload, time-intensity and flexible working hours.
Conclusion: Maintaining a healthy business continuity during this pandemic and the future ones will help promote and protect the wellbeing and safety of employees and their families.
Preparation and characterization of dental pit and fissure sealant based on calcium sodium silicate bioactive glasses
Authors: Safwat Engie M. , Alkabani Yasmine M. , Zaki Dalia Y. , Elbatanony Mai M. , Abd-Elsatar A. G. , Saleh Reham S. , Khater G. A. 
Published: January 2023

Abstract
This study aimed to prepare a resin based dental sealant loaded with novel bioactive glass formulated from (50 wt% calcium silicate and 50 wt% sodium silicate) with different percentages of fluorapatite. Four glass batches were formulated then characterized using Fourier Transform Infrared Spectroscopy analysis, X-ray powder diffraction analysis and Transmission electron microscopy. Density, microhardness and bioactivity testing after insertion in artificial saliva were done. Four Bisphenol-A glycerolate dimethacrylate based sealant loaded with the glass batch that showed the preeminent properties and silica fillers were prepared. The prepared sealants were compared to commercial sealants regarding flow, curing depth, compressive strength and microhardness. Sealant composition that showed comparable properties to that of the commercial sealant was selected for pH changes, ion release testing after immersion in artificial saliva for different time intervals. Results indicated the structural stability, amorphous nature, and bioactive behavior with apatite forming ability of the tested glass batches. Experimental sealant revealed comparable tested properties with lower compressive strength compared to the commercial sealants at P < 0.001. The mean pH values of the tested sealants ranged from 6.75 to 6.35 with extended calcium and phosphorus ion release up to 90 days. It was concluded that the 85 wt% calcium silicate and sodium silicate with 15 wt% fluoroapatite had the best trend regarding ion release and appetite forming ability. Sealant loaded with 65 wt% bioactive glass, 10 wt% sintered nanosilica and 10 wt% nanosilica had the best acceptable mechanical properties. The novel pit and fissure sealant is a promising bioactive and ion releasing material.
The survival outcome and gender difference of elderly females with laryngeal cancer
Authors: Pan Yafeng , Hong Yuming , Qiu Enhui , Ruan Shenjiong 
Published: January 2023

Abstract
Background
To analyze the gender differences in epidemiology and survival of elderly females with laryngeal cancer, and possible clinical countermeasures to reduce the differences.
Methods
Patients aged ≥ 65 years with laryngeal cancer in the Surveillance, Epidemiology, and End Results database from 2010–2018 were collected. The Kaplan-Meier method was performed to calculate overall survival (OS) and evaluate univariate factors affecting OS. Multivariate Cox regression analysis was conducted on variables with P-value < 0.05. Propensity score matching was applied to reduce the effects of confounding factors. Gender differences were analyzed by the chi-squared test.
Results
The gender difference was significant in survival prognosis in the elderly population, with males having significantly better OS than females. The gender difference was mainly manifested in stage I. Gender, age at diagnosis, marital status, tumor site, and treatment modality were prognostic factors affecting the OS of stage I patients. Compared to male patients, females were diagnosed at an older age, with more singles, more supraglottic types, and more likely to undergo lymph node dissection (LND). The gender difference of OS in radiotherapy (RT) group was the most obvious, and the prognosis of organ preservation surgery (OPS) group was comparable to that of the OPS + RT group, but both better than that of the RT group.
Conclusion
The gender difference in survival was significant in elderly patients with stage I laryngeal cancer. To narrow gender differences: LND should be emphasized in females with supraglottic laryngeal cancer; OPS should be considered for females for a better prognosis and combination therapy was not advocated; Age at diagnosis ≥ 85 years and single females should receive more attention.
The SLAPTAG: A new molecular tag adapted for the development of a high-performance, low-cost, affinity chromatography system
Authors: Muruaga Emanuel Javier , Uriza Paula Jimena , Eckert Gonzalo Axel Klauss , Pepe Maria Victoria , Duarte Cecilia Magali , Roset Mara Sabrina , Briones Gabriel 
Published: January 2023

Abstract
The SLAPTAG is a novel molecular TAG derived from a protein domain present in the sequence of Lactobacillus acidophilus SlpA (SlpA284–444). Proteins from different biological sources, with different molecular weights or biochemical functions, can be fused in frame to the SLAPTAG and efficiently purified by the specific binding to a bacterial-derived chromatographic matrix named here Bio-Matrix (BM). Different binding and elution conditions were evaluated to set an optimized protocol for the SLAPTAG-based affinity chromatography (SAC). The binding equilibrium between SLAPTAG and BM was reached after a few minutes at 4oC, being the apparent dissociation constant (KD) of 4.3 uM, a value similar to the one determined for other S-layer proteins and their respective bacterial cell walls. A reporter protein was generated (H6-GFP-SLAPTAG ) to compare the efficiency of SAC against a commercial system based on a Ni2+-charged agarose matrix, observing no differences in the H6-GFP-SLAPTAG purification performance. The stability and reusability of the BM were evaluated, and it was determined that the matrix was stable for more than a year, being possible to reuse it five times without a significant loss in the efficiency for protein purification. Alternatively, we explored the recovery of bound SLAP-tagged proteins by proteolysis using the SLAPASE (a SLAP-tagged version of the HRV-3c protease) that released a tag-less GFP (SLAPTAG-less). Additionally, iron nanoparticles were linked to the BM and the resulting BMmag was successfully adapted for a magnetic SAC, a technique that can be potentially applied for high-throughput-out protein production and purification.
Steroidal Saponin Profiles and Their key Genes for Synthesis and Regulation in Asparagus officinalis L. by Joint Analysis of Metabolomics and Transcriptomics
Authors: Cheng Qin , Zeng Liangqin , Wen Hao , Brown Sylvia E , Wu He , Li Xingyu , Lin Chun , Liu Zhengjie , Mao Zichao 
Published: January 2023

Abstract
Background: Asparagus officinalis L. is a worldwide cultivated vegetable enrichening in both nutrient and steroidal saponins with multiple pharmacological activities. The upstream biosynthetic pathway of steroidal saponins (USSP) for cholesterol (CHOL) synthesis has been studied, while the downstream pathway of steroidal saponins (DSSP) starting from cholesteroland its regulation in asparagus remained unknown.
Results: Metabolomics, Illumina RNAseq and PacBio IsoSeq strategies were applied to different organs of both cultivated green and purple asparagus to detect the steroidal metabolite profiles & contents and to screen their key genes for biosynthesis and regulation. The results showed that there is a total of 427 compounds, among which 18 steroids were detected with fluctuated concentrations in roots, spears and flowering twigs of two garden asparagus cultivars. The key genes of DSSP include steroid-16-hydroxylase (S16H), steroid-22-hydroxylase (S22H) and steroid-22-oxidase-16-hydroxylase (S22O-16H), steroid-26-hydroxylase (S26H), steroid-3-β-glycosyltransferase (S3βGT) and furostanol glycoside 26-O-beta-glucosidases (F26GHs) which were correlated with the contents of major steroidal saponins were screened, and the transcriptional factors (TFs) co-expressing with the resulted synthetic key genes, including zinc fingers (ZFs), MYBs and WRKYs family genes were also screened .
Conclusions: Based on the detected steroidal chemical structures, profiles and contents which corelated the expressions of screened synthetic and TFs genes, the full steroidal saponin synthetic pathway (SSP) of asparagus including its key regulation networks was proposed at first time.
Effect of Rented Warehouses for Deteriorating Items Under Stock Dependent Demand, Partial Backlogging
Authors: sanju Sanjay Sharma , tyagi Anand Tyagi , bb BB Verma , sachin Sachin Kumar 
Published: January 2023

Abstract
In the area of two warehouses-based inventory models, various study has been carried out, authors presented their models under different parameters and environmental conditions. In most of the study, it can be seen that one warehouse assumed as own warehouse and other assumed as rented. Due to different facility and conditions both the warehouses have different rate of demand and deterioration along with different storage capacity. Shilpi et al. [2019], has developed an inventory model by considering both the warehouses as ranted. It was also assumed in the study that both warehouses having the same capacity. In addition, it has been also assumed that the items in the second ranted warehouse start decaying after some time whereas the items in the first warehouse used first to meet the demand of the customers. After critically reviewed the model by Shilpi et al. [2019], it can be observed that the storage techniques of items need to be further explored, as model used the items from first warehouse to satisfy the demand of the customers wares the items whose deterioration about to start has been stored in the second warehouse. This concept of storage has to be change, as such items in the second warehouse totally deteriorated till their turn comes up. Keeping this fact in mind, we have developed an inventory model based on two different ranted warehouses for deteriorated items whose rate of deterioration is not so much high but after a very short of time these items start decaying. According to the current model, the demand of the customers has been satisfied from the first warehouse, also to avoid deterioration in the second warehouse, we have to transfer such items whose deterioration about to start from second warehouse to first ranted warehouse so that we can avoid the deterioration of the items. In the current model we have assumed both the warehouses as rented which is suitable for the manufacturer whose product demand is very high and space is not much enough. It is obvious that the demand for the items in the stock increases due to stock up to a certain level. In the model the demand for the first warehouse is assumed as stock dependent. When we have extra stock the second warehouse used for the products and we have also assumed that the demand has becomes constant. The model is also instigated under sensitivity analysis and a brief numerical example with real data has been illustrated.
Kiln Predictive Modelisation for Performance Optimization
Authors: BENCHEKROUN MOHAMMED TOUM , ZAKI Smail , ABOUSSALEH MOHAMED 
Published: January 2023

Abstract
Exploiting the power of AI in the heavy industry brought back satisfactory results at the kiln level, machine learning techniques allowed predictive modeling of the baking process using powerful Machine Learning models which had a great impact on the energy consumption and kiln production rate among all the models used. Large amounts of historical data were used after analysis and preparation, for which several methods were applied such as preprocessing and feature selection. All models were tested on 20% of the data, using Mean Absolute Error and Root mean squared error as metrics to evaluate our models in order to identify the influencing variables that contribute most to the increase in energy consumption and kiln production rate.