bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeted Therapies for Breast and Lung Cancers by Using Propolis Loaded Albumin Protein Nanoparticles
Authors: Ghazy Mohamed G.M. , Hanafy Nemany A.N. 
Published: February 2023

Abstract
Cancer is a popular disease among many others that can threaten defiantly human life. This is not only because of its invasiveness but also because of its resistance and the highly effective cost of its treatment. Propolis is rich in natural sources of bioactive and polyphenolic compounds that have proven their strong effect on cancer cells such as MCF-7 and A549 cell lines. Propolis extract was inserted into the Albumin protein, Bovine Serum (BSA) conjugated to folic acid (FA) to increase control of its delivery and to increase their cellular uptake. The growth of MCF-7 and A549 was significantly decreased by propolis extract and BSA-propolis NPs after their incubation for 72h by (54 ± 0.01% and 45 ± 0.005%, P ≤ 0.001) and (20 ± 0.01% and 10 ± 0.005%, P ≤ 0.0001) respectively. Similarly, there is a significant inhibition in the growth of A549 obtained after their incubation (propolis extract and albumin-propolis NPs) for 72 h (15 ± 0.03% and 5 ± 0.01%, P ≤ 0.00001). Propolis extract and BSA-propolis NPs exhibited a greater effect on protein expression of MCF-7 and A549 showing significant modulation of caspase-3, cyclin D1, and LC3II. The result was supported by the presence of nuclear fragmentations and activation of acidic/neutral autophagosomes in AO/EB and DAPI stains. In the recent investigation, the expression of phospho-GSK3β (Ser9) (p < 0.001) increased significantly in MCF-7 and A549 cells after their exposure to propolis extract and BSA-propolis NPs. Results support the potency application of propolis and its encapsulation as an alternative therapeutic agent for cancer treatments instead of chemotherapies because of its action on multi-signaling pathways.
Isolation and structure elucidation of the compounds from Teucrium hyrcanicum L. and the investigation of cytotoxicity, antioxidant activity, and protective effect on hydrogen peroxide-induced oxidative stress
Authors: Ghasemi Saeed , Evazalipour Mehdi , Peyghanbari Nastaran , Zamani Ehsan , Bellstedt Peter , Molaee Mahan , Koohi Diba Eghbali , Yousefbeyk Fatemeh 
Published: February 2023

Abstract
Background: Teucrium hyrcanicum L. (family Lamiaceae) is widely distributed in the north and northwest of Iran. It has been used in form of tea, tonic, and tincture for the treatment of various diseases such as cough, rheumatism, and fever.
Methods: In this study, the total phenolic and flavonoid contents, antioxidant and cytotoxic activities of total extract and different fractions of T. hyrcanicum were measured. Furthermore, the potential ability of T. hyrcanicum to protect H2O2-induced oxidative stress was tested on NIH3T3 cell line. Then, the isolation and structure elucidation of the compounds were performed on the most potent fractions. Finally, the quantification of isolated compounds in total extract (TE) was done by HPLC method. Isolated phytochemicals were assessed for cytotoxic and antioxidant activity.
Results: The results indicated that the methanol fraction (MF) had the highest amount of phenolic and flavonoid contents (69.36 mg GAE/g extract and 68.95 mg QE/g extract). The highest radical scavenging activities were observed from MF and TE (IC50 44.32 and 61.12 μg.ml-1, respectively). The best cytotoxicity was obtained by ethyl acetate fraction (EF) against A431 and MCF7 (IC50 values of 235.4and 326.6 μg.ml-1, respectively). The pretreatment with MF exerts the highest reduction in malondialdehyde (MDA) formation (IC50= 2.51 μM, p<0.001) compared to the H2O2 group (5.77 μM). Also, MF significantly inhibited H2O2-induced Glutathione (GSH) oxidation (p<0.001). Furthermore, two phenolic compounds, including acteoside and quercetin were isolated and identified in MF and EF, respectively. The IC50 values of acteoside and quercetin in DPPH assay were 7.19 and 5.56 µg.ml-1, respectively. Both quercetin and acteoside significantly reduced the MDA formation and inhibited GSH oxidation which was comparable with BHA (as standard antioxidant) (p< 0.05). Acteoside demonstrated significant cytotoxicity against all tested cell lines (IC50=32 to 145 μg.ml-1). The HPLC quantification of isolated compounds revealed that the quantity of acteoside and quercetin in TE were 93.31 and 16.87 μg.mg-1, respectively.
Conclusions: The isolated compounds (quercetin and acteoside) had significant antioxidant activity and revealed protective effect on H2O2-induced oxidative stress which was comparable with BHA.
Fuzzy model multi-attribute decision-making under information systems
Authors: Sun Zhenduo , Kong Xiangzhi 
Published: February 2023

Abstract
This paper combines information systems with fuzzy pattern recognition and proposes an improved fuzzy pattern decision-making method based on it. The decision attributes and conditional attributes are selected according to the decision purpose, and the correlation coefficients of the decision attributes and conditional attributes are derived by combining the original attribute values using the correlation method to determine the weights of each conditional attribute, while the weights of the decision attributes are determined by the decision maker. The standard set and the set to be measured in the fuzzy model are constructed using the affiliation functions of the different attributes. The degree of proximity between the criterion set and the set to be tested is calculated and the solution that best exploits the advantages of the current stage is selected as the optimal solution. The introduction of an information system into the pattern recognition model simplifies the decision-making process by reducing the number of attributes that may be missed in the decision and retaining the characteristics of the original attributes.
The Long-term Effectiveness of Sacral Neuromodulation in Treating Low Anterior Resection Syndrome: A Single Center Experience
Authors: Bisgin Tayfun , Canda Aras Emre , Manoglu Berke , Ellidokuz Hulya , Sokmen Selman 
Published: February 2023

Abstract
Background
Sacral neuromodulation (SNM) has emerged as an effective treatment option for patients with fecal incontinence. The efficacy of SNM in the treatment of low anterior resection syndrome (LARS) following rectal cancer surgery is encouraging. The aim of this study is to review the long-term outcomes of patients treated with SNM for LARS.
Methods
A review of a prospectively maintained database of consecutive SNM procedures for LARS between June 2017 and June 2020 was conducted. Bowel habits diaries, the Cleveland Clinic Florida-Fecal Incontinence score (CCF-FIS), the Fecal Incontinence Quality of Life Scale (FIQoL), and the LARS score were evaluated at baseline, three months, and twenty-four months after definitive SNM implantation.
Results
The study included 14 patients; 11 of them were males, and the mean age was 59.2 (±10.2). Thirteen patients underwent permanent implantation of the SNM device. The mean score of FI episodes were reduced from 16 to 4 (p < 0.001) and the mean CCF-FIS dropped from 15.2 to 6.5 (p < 0.001). All patients showed a substantial increase in their FIQoL scale (p < 0.001). Additionally, there was a significant amelioration in the LARS score (36.7 to 17.3, p < 0.001) and all symptoms of LARS except incontinence of liquid stool (p = 0.97).
Conclusions
SNM improves bowel dysfunction and quality of life in patients with LARS following rectal cancer surgery and maintains its effectiveness over time
Analysis of the effect of heavy metals on the incidence of cancer and health risk assessment on drinking water sources in 2021-2022 using geostatistics (Study area: Kohgilouye and BoyerAhmad province, Iran)
Authors: Barafrashtehpour Mansour , Ebrahim Afshin , Javad Tarrahi Mohammad , Asadi Morteza , Pourzamani Hamidreza 
Published: February 2023

Abstract
In this study, zoning and the effect of heavy metal concentration on the incidence of cancer, the effect of land use, the location of quifers, the assessment of carcinogenic and non-carcinogenic health risks of heavy metals for 104 drinking water sources and 1248 samples based on standard sampling methods (using It was measured by atomic absorption method equipped with graphite furnace) from drinking water sources. Then statistical indicators and spatial information system were used to compare and analyze the data. According to the obtained results, the highest concentrations of arsenic, chromium and zinc correspond to Bashet, Behmai and Cheram cities respectively (15.47 ± 10.52, 292.21 ± 312.29, 599.09 ± 320.74 micrograms per liter) Is; that the concentration of arsenic and chromium in some sources is higher than the standard. One-way analysis of variance showed that there is a significant difference between the average concentration of arsenic, chromium and zinc in the cities (P. Value < 0.05). The average health risk factor of arsenic in Bashet and Chrome in Behmai city is higher than 1. According to the results of the average cancer risk of arsenic in the drinking water sources of Basht, Gachsaran and Behmai and chromium in the water sources of Behmai and Gachsaran have a definite risk of cancer.
The roles of hospital cleaning staff in the care of infectious patients and social health during the infectious diseases disasters: a qualitative content analysis study
Authors: Ebrahimian Abbasali , Rahbar Ahmad 
Published: February 2023

Abstract
Background: This study aimed to explain the roles of hospital cleaning staff in caring for infectious patients and social health during infectious disease disasters.
Methods: The present study was conducted by qualitative content analysis. Participants were fifteen cleaning staff of public hospitals in Qom, Iran. Data were collected using semi-structured interviews.
Results: Data analysis led to the main theme: "Selfless efforts to help provide comprehensive patient care and disease prevention in society." This theme was derived from three main categories "helping nurses to improve the quantity and quality of basic care," "selfless service," and "helping to prevent infectious diseases in society."
Conclusions: In infectious disasters, cleaning staff provides considerable help to comprehensive patient care. They can respond to infectious disease disasters in society as a practical model of personal hygiene and adhere to health protocols. Nursing managers can use the capabilities of cleaning staff in the response phase of infectious disasters.
Electroacupuncture alleviates motor dysfunction and gut barrier damage by modulating intestinal NLRP3 inflammasome in MPTP-induced Parkinson's disease mice
Authors: Guo Lei , Hu Haiming , Jiang Nan , Yang Huabing , Sun Xiongjie , Xia Hui , Ma Jun , Liu Hongtao 
Published: February 2023

Abstract
Parkinson’s disease (PD) is a neurodegenerative disorder commonly accompanied by motor dysfunction. Electroacupuncture (EA) has shown anti-inflammatory and neuroprotective effects, although the potential mechanisms remain unclear. We speculated that EA could ameliorate the motor dysfunction of PD and that this would be associated with its regulatory impact on the intestinal microbiota. We applied 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to establish a PD mouse model. EA at the GV16, LR3, and ST36 acupoints was administrated for 12 consecutive days. The results of the open-field test indicated that EA alleviated depression and behavioral defects in PD mice. Further study showed that EA upregulated the expressions of tyrosine hydroxylase (TH) and brain-derived neurotrophic factor (BDNF), and blocked the accumulation of α-synuclein (α-syn) in the midbrain. Additionally, EA increased the mRNA levels of neurotrophic factors like BDNF and GDNF. Moreover, EA prevented the damage to intestinal tissues of PD mice, indicative of suppressed NLRP3 inflammasome activation and increased gut barrier integrity. Notably, the antibiotic-treated mouse experiment validated that the gut microbiota was critical in alleviating PD dyskinesia and intestinal inflammation by EA. In conclusion, our study demonstrated that EA intervention could improve PD by alleviating behavioral defects and gut barrier damage, which provides novel insights into the pathogenesis of PD and its therapy.
Leptin Signalling Promotes Axonal Regeneration in the Peripheral and Central Nervous System
Authors: Chadwick Jessica , Serger Elisabeth , Kong Guiping , Zhou Luming , Mueller Franziska , Palmisano Ilaria , Liddell Phoebe , Chu Linshan , Yan Yuyang , Giovanni Simone Di 
Published: February 2023

Abstract
Axonal growth is limited or absent following peripheral or central nervous system injury respectively, inhibiting repair. The identification of novel growth-promoting molecular mechanisms is therefore a priority. In the search for dietary-dependent mechanisms that control neuronal regenerative ability, we discovered that growth-promoting intermittent fasting (IF) induced leptin expression in sensory neurons of the dorsal root ganglia (DRG). Surprisingly, leptin signalling, whose canonical function is to control energy homeostasis, was found to be central to the IF-dependent regenerative phenotype. In fact, neuronal conditional deletion of the leptin receptor significantly impaired the regenerative response elicited by IF. Overexpression of leptin in vivo in DRG neurons enhanced axonal regeneration following peripheral sciatic nerve crush (SNC) and central spinal cord injury (SCI). Lastly, RNA sequencing following leptin overexpression in DRG neurons showed a significant increase in regenerative gene expression and transcription after SCI, indicating a role for leptin in inducing a euchromatic, transcriptionally active environment that facilitates nervous system repair after injury.
Demonstration of Reverse Designs based on Hand-drawn Electrical Characteristics in GaN Power Devices using Graph Attention Networks
Authors: Tseng Yi-Ming , Chen Bang-Ren , Lin Wei-Cheng , Lee Wen-Jay , Chen Nan-Yow , Wu Tian-Li 
Published: February 2023

Abstract
In this work, the methodology for the reserve design in GaN power MIS-HEMTs based on hand-drawn characteristics is demonstrated for the first-time using Graph Attention Network. The hand-drawn ID-VG characteristic is constructed by Ramer-Douglas-Peucker algorithm. Then, the extracted information is sent to the Graph Attention Network (GAT) to receive the corresponding device design variables, including tAlGaN, recessed depth, Al%, Lg, Lgd, and Lgs. Less than 8% of the differences in the key extracted parameters, such as Vth, Ion, and subthreshold slope (SS), can be obtained and less than 30 seconds is consumed to generate the design variables. Therefore, the developed GAT approach is promising for the reverse design of GaN power MIS-HEMTs, which can provide users with efficient and valuable design suggestions to optimize the devices toward the targeting performance.
Estimating the impact of the COVID-19 pandemic on dengue in Brazil
Authors: Roster Kirstin Oliveira , Martinelli Tiago , Connaughton Colm , Santillana Mauricio , Rodrigues Francisco 
Published: February 2023

Abstract
Atypical dengue prevalence was observed in 2020 in many dengue-endemic countries, including Brazil. Evidence suggests that the pandemic disrupted not only dengue dynamics due to changes in mobility patterns, but also several aspects of dengue surveillance, such as care seeking behavior, care availability, and monitoring systems. However, we lack a clear understanding of the overall impact on dengue in different parts of the country as well as the role of individual causal drivers. In this study, we estimated the gap between expected and observed dengue cases in 2020 using an interrupted time series design with forecasts from a neural network and a structural Bayesian time series model. We also decomposed the gap into the impacts of climate conditions, pandemic-induced changes in reporting, human susceptibility, and human mobility. We find that there is considerable variation across the country in both overall pandemic impact on dengue and the relative importance of individual drivers. Increased understanding of the causal mechanisms driving the 2020 dengue season helps mitigate some of the data gaps caused by the COVID-19 pandemic and is critical to developing effective public health interventions to control dengue in the future.
Functional Characterization of a missense mutation in the TRAPPC12 gene presenting with progressive encephalopathy with brain atrophy and spasticity phenotype without microcephaly and epilepsy
Authors: Yucesan Emrah , Goncu Beyza , Yesil Gozde , Aslanger Ayca Dilruba 
Published: February 2023

Abstract
Background
Variations in the TRAPPC12present many symptoms, including microcephaly, agenesis of corpus callosum, cerebellar atrophy, and epilepsy. These features indicate a broad range of mortality and morbidity. Identifying a variation with functional consequences provides an accurate diagnosis and proper counseling for the family. Herein, we describe the functional analysis of the homozygous missense variation NM_016030.6:c.679T>G in the TRAPPC12.
Methods
CCD1079Sk cell line (as a healthy control) and patient-derived fibroblast cells were evaluated for Golgi integrity, ER structure, and vesicle distribution and protein levels by Western blot.
Results
Protein expression showed an absence in the TRAPPC12 protein and an uncharacterized protein fragment (CGI-87). The lack of Golgi integrity and enlargement of ER structure were shown, and the vesicle distribution was even more disrupted via truncated TRAPPC12 protein. The effect of the variant resulted in milder clinical phenotype with the absence of microcephaly and epilepsy, which we had previously reported.
Conclusion
We have shown that the protein expression was disrupted, Golgi and ER structure negatively affected, resulting in a decreased and scattered number of vesicles while also having alterations in vesicle distribution. Nevertheless, these impairments did not arrest vesicle trafficking due to protein loss. However, unlike previous reports, we did not show a vesicle accumulation.
Anti-inflammatory and Ameliorative effects of two newly isolated probiotic strains in DSS-induced mouse model of colitis
Authors: Ghorbani Elnaz , Rezaei Shaghayegh , Soleimanpour Saman , Nazari Seyedeh Elnaz , Rahmani Farzad , Naimi Hamideh , Afshari Asma , Avan Amir , Ryzhikov Mikhail , Ferns Gordon , Khazaei Majid , Hassanian Seyed Mahdi 
Published: February 2023

Abstract
Background: Protective effects of probiotics in human diseases have been well documented in recent years. In this study, the anti-inflammatory and fibrinolytic properties of two newly isolated probiotic bacteria, Lactiplantibacillus plantarum and Lactococcus lactis either alone or in combination with standard therapy, Mesalazine (MSZ), have been investigated in a murine model for ulcerative colitis.
Methods: Characterization of newly isolated probiotic were assessed by performing antibacterial activity, antibiotic resistance, acid and bile tolerance, and hemolytic activity assays. Hematoxylin-Eosin and Masson's trichrome staining were used to evaluate inflammation and collagen deposition in colon tissue sections. Expression of inflammatory- and Fibrotic-associated genes were analyzed using Real-time PCR and ELISA assays.
Results: Results showed that administration of probiotics significantly attenuated DSS-induced colon shortening, colon weight loss, and increase in spleen weight in colitis mice. Compared to the colitis mice, disease activity index as assessed by changes in body weight, degree of stool consistency, rectal bleeding, and prolapse was decreased in probiotic-treated group. Mix of probiotics potently improved histopathological score by attenuating crypt loss, mucosal damage, and inflammation score in colitis tissues. Consistently, mRNA and protein levels of pro-inflammatory genes as well as oxidative stress markers were suppressed in the presence of probiotics in colon tissues. Furthermore, probiotic bacteria reduced fibrosis by down-regulating pro-fibrotic genes including Col 1a1 and α-SMA in colon tissue homogenates.
Conclusion: These results showed that mix of these newly isolated probiotic bacteria is as potent as the standard treatment and could be utilize as a novel therapy for ulcerative colitis without any observed side effects. Future experiments on these probiotics in other models of UC is needed to completely address the efficacy potency and safety concerns.
The Quagmire Model: How a small step can trap you in the quagmire of misinformation, hate speech and denialism
Authors: Camilo-Da-Silva Eduardo , Barbedo Claudio Henrique Silveira , Marins Eugenio Rangel , Santini Rose Marie , Castro Joel Pereira 
Published: February 2023

Abstract
This study aims to explain how social media (SM) users, whilst searching for information, can be trapped into a quagmire of misinformation, even when they have no denialist inclinations or sympathy for hate groups. We analyze the interactions between cognitive biases and deep preference learning algorithms (DPL), as SM companies use DPL to curate the content conveyed to its users. The study proposes a model for users’ behavior and explain how SM business model allows new information to be introduced in the quagmire in order to change user’s opinions in a way desired by a customer willing to pay for, and, eventually, accomplished it. The model explains why some popular tactics against misinformation, as censorship and fact-checking, achieve very poor results. We suggest that policies promoting face-to-face encounters in friendly environments can be more effective in that struggle. We believe the model can help decision makers in developing more efficient anti-disinformation policies.
Design and Development of Insole Using Carbon Fiber For Partial Foot Amputation
Authors: Wagle Chandrika , Padhi S. N. 
Published: February 2023

Abstract
One recommended gait rehabilitation treatment after stroke is using ankle and foot orthotics. In clinical practice, it is essential to adjust the torque of the ankle-foot orthosis assistance to suit each patient’s body function and gait ability. The present study investigated the effect of changing the plantar flexion resistance of the ankle–foot orthosis on the post-stroke gait kinematics and kinetics during the early stance phase using a musculoskeletal model and an ankle–foot orthosis model. A prosthetic foot plate is designed, analyzed, and validates with the experiment work accomplished in this paper. A finite element (FE) model of the human foot was developed from 3D printing of 2 mm thick UK7 size. The finite element predictions are being validated theoretically and experimentally.