bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sodium aescinate reduces the incidence of Prolonged postoperative ileus after open surgery of advanced gastric cancer
Authors: Liu Yu , Sui Chao , Tao Liang , wang Meng 
Published: June 2023

Abstract
Background
Prolonged postoperative ileus (PPOI) is one of the common complication postoperative complications of gastric cancer (GC) surgery, with limited preventive and treatment options currently available. This study aims to investigate the therapeutic efficacy of sodium aescinate in managing PPOI and identifying the related risk factors of PPOI in GC patients.
Methods
A total of 121 patients with advanced GC were enrolled and assigned to two groups to receive sodium aescinate or no treatment. Univariate and multivariate analyses were used to identify the PPOI-related risk factors in postoperative GC patients.
Results
The study revealed that postoperative sodium aescinate treatment independently served as a protective factor against PPOI (OR = 0.25, CI: 0.07–0.86, P = 0.028). Furthermore, the incidence of PPOI was significantly lower in patients who received sodium aescinate treatment compared to those who did not (15/66 VS 4/55, P = 0.020).
Conclusions
Postoperative administration of sodium aescinate reduces the occurrence of PPOI in patients with advanced GC.
Comparative proteomics analysis of the responses to cigarette smoke particulate matter in six plant species leaves
Authors: Permana Bayu Hadi , Nookongbut Phitthaya , Krobthong Sucheewin , Yingchutrakul Yodying , Saithong Treenut , Thiravetyan Paitip , Treesubsuntorn Chairat 
Published: June 2023

Abstract
Phytoremediation has been recognized to reduce particulate matter (PM) concentration in the air. Plant stress response plays a crucial role in PM removal. In this study, six plants including ornamental and perennial plants were exposed to PM from cigarette smoke for 24 hours. Ornamental plants were Calathea makoyana, Sansevieria trifasciata, and Zamioculcas zamiifolia, where perennial plants Bauhina purpurea, Tectona grandi, and Wrightia religiosa. The initial concentration of PM was 300–320, 400–450, and 500–530 µg m− 3 for PM1.0, PM2.5, and PM10, respectively. The plant response of each plant was compared to determine the plant response of each group and plant against PM stress. The results showed that all plants have different protein expressions. Ornamental plants showed more enrichment in photosynthesis and antioxidant enzymes, whereas perennial plants were in photosynthesis, an antioxidant enzyme, and carbon metabolism. The results suggest the plant response of each plant of an ornamental and perennial plant. This proteomics approach was useful for future studies, especially for phytoremediation.
Healthy Life Skills and Related Factors Among University Students: A Cross-Sectional Study in İstanbul, Turkey
Authors: Rashidi Mahruk , Karaman Funda , Yıldırım Gülay , Genç Aslı , Jafarov Gülşah Ünsal , Çakmak Sultan 
Published: June 2023

Abstract

Background:Chronic non-communicable diseases (NCDs) are the leading cause of global deaths. Unhealthy lifestyle choices contribute significantly to the development of NCDs. University students, in particular, are considered a high-risk population for NCDs due to the transitional phase they experience. Adopting and maintaining healthy behaviors during this period is crucial for their overall well-being and future health outcomes.Aims:This study was conducted to determine healthy life skills in university students.Methods:A cross-sectional study was conducted at a university in Istanbul. A questionnaire measuring healthy life skills was administered. Data were analyzed using various statistical tests, including chi-squared tests, t-tests, Mann-Whitney U test, one-way ANOVA test, univariate, and regression analysis. The significance level for all statistical tests was set at p < 0.05.Results:A total of 547 students participated in the study. The mean age of the participants was 21.6±2.9 years, with 51.4% male and 48.6% female students. The average score for healthy life skills was 63.5 out of 84. Significant differences were found in the scores based on marital status, economic income, social security insurance coverage, and educational grade. Students who reported alcohol and smoking consumption had lower scores.Conclusion:The study revealed that university students in Istanbul possess a satisfactory level of healthy life skills. By providing social support, such as expanding the coverage of social security insurance and establishing conducive educational environments, while also paying attention to the influence of peers on students, we can contribute to the development of healthy life skills in university students.
Identification of protein-degraders in an anaerobic digester by protein stable isotope probing combined with metagenomics
Authors: Deng Zhe , Poulsen Jan Struckmann , Nielsen Jeppe Lund , Weissbrodt David G. , Spanjers Henri , Lier Jules B. 
Published: June 2023

Abstract

BackgroundPresence of carbohydrates hampers protein degradation in anaerobic digesters. To understand this phenomenon, we used proteogenomics to identify the active protein-degraders in the presence of low and high carbohydrates concentrations. Active metabolic pathways of the identified protein-degraders were investigated using proteomics with13C-protein substrates (protein stable isotope probing).ResultsAcinetobacterwas identified as the main protein-degraders under both protein-fed and protein-glucose mixture-fed conditions. The incorporation of the13C-labelled protein substrate was predominantly observed in outer membrane-bound proteins and porin proteins, which are associated with proteinases or the transportation of amino acids across the cell wall. TheAcinetobactermetabolic model and the incubation conditions suggested that glucose and proteins were degraded through anaerobic respiration.ConclusionsThe unfavourable effect of the presence of carbohydrates on protein biodegradation was attributed toAcinetobacter’s preference for substrate. This work highlights that efficient degradation of protein and carbohydrate mixtures in anaerobic digesters requires a staged or time-phased approach and enrichment of active protein-degraders.
Clinical significance of TMEM229A Q200del mutation in lung adenocarcinoma
Authors: Liang Yixian , Xie Yanping , Yu Huanming , Zhu Wenjuan , Yin Chengyi , Zhang Xilin , Dong Zhaohui 
Published: June 2023

Abstract
Lung adenocarcinoma is one of the major histopathological subtype of non-small cell lung cancer (NSCLC), including solid, acinar, lepidic, papillary and micropapillary subtypes. Increasing evidence had showed that micropapillary lung adenocarcinoma was positively associated with higher incidence of metastasis and poorer prognosis, while lepidic lung adenocarcinoma had a relatively better prognosis. However, the key alteration signatures and its role in micropapillary lung adenocarcinoma progression are not exactly determined. Here, 181 patients with lung adenocarcinoma who underwent surgery in the First Affiliated Hospital of Huzhou University from January 2016 and December 2020 were retrospectively enrolled. And three lepidic and three micropapillary lung adenocarcinoma samples were sequenced using whole-exome sequencing. More comprehensively analyze genomic variations between lepidic and micropapillary lung adenocarcinoma was performed. In addition, TMEM229A Q200del mutation was verified using our cohort and The Cancer Genome Atlas-Lung Adenocarcinoma (TCGA-LUAD) datasets. The correlations between TMEM229AQ200del mutation and clinicopathological characteristics of patients with lung adenocarcinoma were further analyzed. The functions of TMEM229A Q200del in H23 cell proliferation and migration were also determined. As expected, the frequency of genomic alteration signatures in patients with micropapillary lung adenocarcinoma was higher than that in lepidic lung adenocarcinoma. Mutations in EGFR, ATXN2, C14orf180, MUC12, NOTCH1 and PKD1L2 were concomitantly detected in three micropapillary and three lepidic lung adenocarcinoma cases. But TMEM229A Q200del mutation was only mutated in lepidic lung adenocarcinoma. Additionally, TMEM229AQ200del mutation was observed in 16 cases (8.8%) of our cohort, while TMEM229A mutations (R76H, Q200del and M346T) accounted for approximately 1.0% of cases in TCGA-LUAD cohorts. Further correlation analysis between TMEM229AQ200del mutation and clinicopathological characteristics suggested that lower frequency of Q200del mutation was significantly associated with gender, positive of lymph node metastasis, advanced TNM stage, positive of cancer thrombus and pathological patterns. Finally, forced overexpression of TMEM229AQ200del markedly suppressed H23 cell proliferation and migration in vitro. In summary, our results demonstrated that TMEM229AQ200del mutation plays a protective role in the progression of lung adenocarcinoma, which could be helpful in developing a novel therapeutic target in lung adenocarcinoma.
Examining Sociodemographic Disparities in Maternal Health in Brooklyn via an Obstetric Outcomes Database
Authors: Tang TzeYin , Smith Michelle , Kernizan Faradia , Friedman Monica , Afable Aimee , Pardo Christina , Clare Camille A. 
Published: June 2023

Abstract

BACKGROUND
Socioeconomic inequities in obstetrics, which manifest as differential rates of maternal adverse events, have been elucidated in past studies. Our obstetric database was created to examine the link between social determinants of health (SDOH) and maternal outcomes in our community in Central Brooklyn, New York, which consists predominantly of the Black and Afro-Caribbean population.
METHODS
Our obstetric database collects information on six categories: 1) severe maternal morbidity events, 2) demographics, 3) prenatal care initiation, 4) risk factors, 5) delivery outcomes, and 6) postpartum follow-up. Data was extracted retrospectively from records at an area hospital in 2019. A data dashboard with analyses on SDOH variables and obstetric outcomes via logistic regression is regularly maintained.
RESULTS
In our population, the prevalence of postpartum hemorrhage, gestational diabetes mellitus, gestational hypertension, preeclampsia, and chronic hypertension are 9.6%, 11.2%, 4.1%, 7.4% and 3.7% respectively. Furthermore, our patients have reported healthcare barriers related to inadequate insurance coverage, transportation to healthcare facility, and childcare. Furthermore, postpartum visit non-attendance was significantly associated with health care barriers (p<0.05), unemployment during pregnancy (p<0.05) and Medicaid usage (p=0.05).
CONCLUSIONS
Our data reveals links between SDOH and postpartum visit attendance, but not obstetric outcomes. Continuous data tracking can reveal the impact of SDOH on obstetrical outcomes and enable institutions to devise initiatives to mitigate health inequities.
Classification of MR Images pertaining to with artifact / without artifact using Deep Autoencoder Convolutional Neural Network
Authors: Samuel Shiju , Ochawar Rohini S. , Rukmini M.S.S 
Published: June 2023

Abstract
Today's radiologists are highly concerned with motion artifact available in MR images and expect MR images during diagnosis without motion artifact. Radiologists receive merged data of MR images with and without motion at the time for patient diagnosis. The radiologist may be mislead by these images of motion artifacts. We suggest a classification model in this paper that makes use of a deep autoencoder convolutional neural network (DAE-CNN) and has three levels as (i) input level (ii) hidden level and (iii) output level. We assessed the classification model and the results are analyzed regarding the accuracy in terms of motion/without motion MR Images.
Genetic Diversity and the Effects of the Highway Matrix on the Roadkill Patterns of the Crab-Eating Fox, Cerdocyon Thous (Linnaeus, 1766), in Northeastern Brazil
Authors: Tenório José Cleiton Souza , Cruz Geyner Alves dos Santos , Silveira-Filho Vladimir , Alexandre Fernando Silva , Gripp Tiago Mota , de Araújo Bianca Gonzaga , Lyra-Neves Rachel Maria , Júnior Wallace Rodrigues Telino 
Published: June 2023

Abstract
The development of modern society depends on the connectivity of different regions, and in particular, the establishment of roads and highways. While roads may be fundamental to development, they tend to have negative impacts on the biodiversity of a region. The present study verifies the roadkill patterns of the crab-eating fox, Cerdocyon thous, in the context of the characteristics of the surrounding matrix. Specimens were collected from four highways in the Brazilian states of Pernambuco and Alagoas, between August 2019 and December 2020. The date, time, and location of each specimen was recorded, and the carcasses were removed from the highway to avoid replicating records. A total of 101 specimens were collected, with a 1:1 sex ratio. Roadkill hotspots were observed in environments to which C. thous is adapted, in particular open fields and the margins of areas with denser vegetation. Rainfall may influence the observed roadkill patterns, given that the hotspots coincided with areas that have annual rainfall of between 600 mm and 700 mm. The molecular analyses indicated that the sample collected encompasses individuals from two distinct genetic groups, which are both distributed throughout the study area, reflecting its reduced genetic diversity. This scenario is influenced by factors such as the pattern of anthropogenic impacts within the study area and the ecological characteristics of the species. The impacts of the roadkill hotspots can be mitigated by measures such as signposting during recruitment periods.
Modelling the long-term carbon storage potential from recalcitrant matter inputs in tropical arable croplands
Authors: Díaz Christhel Andrade , Balugani Enrico , Zamora-Ledezma Ezequiel , Hamelin Lorie 
Published: June 2023

Abstract

The urgency to achieve climate neutrality and limit global warming requires a transition to low fossil carbon use. Crop residues, an abundant source of renewable carbon, remain underutilized, among others due to soil conservation practices. Soil organic carbon (SOC) plays a crucial role in tropical croplands by supporting soil health, nutrient availability, and biogeochemical cycles. The incorporation of exogenous organic matter (EOM) amendments has the potential to enhance carbon storage and fertility. This study conducted in Ecuador, a biodiversity hotspot, aims to identify SOC stock vulnerabilities, estimate SOC storage potential and changes in CO2 fluxes in tropical cropping systems resulting from changes in crop residue harvest for use within the bioeconomy, when a subsequent recalcitrant EOM application is involved. A spatially-explicit modeling framework representing the agricultural area into 15,782 agricultural pedoclimatic units was employed to assess the potential for SOC storage and to quantify resulting CO2 emission changes in tropical cropping systems. Four scenarios were analyzed, all implying the conversion of crop residues into bioeconomy products as well as recalcitrant EOMs. The RothC soil model, adapted to incorporate additional carbon pools for labile (CL) and recalcitrant (CR) fractions, as well as the priming effect, was utilized alongside high-resolution data to evaluate SOC storage potential for each scenario. Baseline SOC stocks ranged from 7.43 to 235 t C ha− 1, with an average of 61.76 t C ha− 1. At the national level, the business-as-usual (BAU) scenario, i.e. crop residues removal, projected a potential 4% increase in SOC stocks by 2040 and a 7% increase by 2070. However, SOC stocks decreased in 79% of the study area. The simulations demonstrated the potential to supply 113 PJ biomass for the bioeconomy without incurring SOC losses in the pyrolysis and gasification scenarios. Harvesting residual biomass with co-product return led to a 19–39% reduction in CO2 emissions over 50 years, depending on the scenario. Sensitivity analyses revealed the priming effect as a particularly sensitive parameter for the results.
FSCN1 has a potential indication for the prognosis and regulates the migration of HNSCC
Authors: Wei Xin 
Published: June 2023

Abstract
Background
The study of molecular markers for diagnosis and prognosis is of great clinical significance for HNSCC patients. In this study, we proposed that FSCN1 has a potential indication for prognosis and is essential for the migration of HNSCC.
Methods
We analyzed the expression and survival association of FSCN1 in HNSCC using TCGA data. We compared the expression of FSCN1 in tumors from primary and metastasis HNSCC patients using QPCR, western blotting, and immunochemistry staining. We determined the migration velocity of multiple HNSCC cell lines using a chemotaxis migration assay. We analyzed the correlation between FSCN1 expression and HNSCC cell migration. We also test the effect of FSCN1 knockdown and overexpression on HNSCC cell migration.
Results
FSCN1 was overexpressed in HNSCC than pair normal tissues and metastasis HNSCC than primary HNSCC. FSCN1 expression was associated with significantly poorer overall survival of HNSCC patients. FSCN1 was potentially associated with immune cell infiltration and migration-associated genes. FSCN1 level was correlated with the migration in HNSCC cell lines. Knockdown of FSCN1 reduced the migration and the overexpression of FSCN1 promoted the migration of HNSCC cell lines.
Conclusion
FSCN1 is a potential prognostic marker and a critical biomolecule for the migration of HNSCC
Human nonsense mutations in primary hyperammonemia – Analysis of publicized patient mutations and variations in general populations in eight disease-causing genes
Authors: Kamoshita Nobuhiko , Hiramoto Takafumi , Kashiwakura Yuji , Hayakawa Morisada , Ohmori Tsukasa 
Published: June 2023

Abstract
To dissect nonsense mutations to be treated with tRNAs, variations within eight genes causing primary hyperammonemia were evaluated. Overall, 156 patient mutation and 60 variation sites were reported in 140 publications and in two genomic databases of 114,878 participants, respectively. Counting the frequency revealed that CGA>TGA was by far the hottest spot, accounting for 41% of patient mutations and 50% of nonsense alleles, which were underestimated in preceding database searches but match the observed high CpG mutation rate. Over 20 variations were linked to patient mutations in the same population at both CGA and non-CGA sites, which will be informative for therapy. While values in the database could predict incidence rate of autosomal enzyme deficiencies, most of them were lower than the equilibrium value expected in autosomal recessive genes, which was calculated from germline CpG mutation rate and selection coefficients estimated here. Toxicity inherent to nonsense variations, if expressed during reproduction, explains this observation, although the details need to be elucidated. The presence, maintenance, and pathogenesis of nonsense variations will thus be diverse. Pertinent clinical information including personal genomic data will enrich our understanding of their characteristics.
Refugees through the eyes of locals; a study on tolerance towards outer groups in Turkey and Germany
Authors: Adee Md Iltemas Amin , Lee Grace Hooi Yean , Rahman Habib 
Published: June 2023

Abstract
In 2019, around 1% of the world’s population was displaced, among whom 26 million are expected to be refugees. The arrival of refugees has been related to prejudice and intolerance towards outer groups. This paper investigates the effect of refugee influx on tolerance towards outer groups, immigrants and foreigners, other races, and religions in Europe’s two largest refugee-receiving countries, Turkey and Germany. For this study, individual-level data from the World Value Survey (WVS) and European Value Survey (EVS) is used in a quasi-experimental setup, difference in difference (DID) strategy for the Turkish context, and pre-post intervention regression analysis for the latter. The results showed that the refugee influx positively influenced tolerance in both countries in the short run. However, the Turkish population became less tolerant over time. As the European Union (EU) has a definite role in influencing migration and refugee integration through its laws and funding, individuals with confidence in the EU became more tolerant toward outer groups. The results are robust to several falsification and placebo tests. These findings provide important implications for government bodies and policymakers to promote integration and understanding among diverse communities.
Assessment of Physicochemical and Bacteriological Parameters of Borehole Water: A case study from Lekki, Lagos, Nigeria.
Authors: Akerele Daniel D , Obunadike Callistus , Abiodun Pelumi Olaitan 
Published: June 2023

Abstract
Access to clean and wholesome water is one of the fundamental human rights, despite billions of people around the world still lacking access to this basic necessity. In this study, a comprehensive analysis of the physicochemical and bacteriological parameters of borehole eater was carried out, using Lekki in Lagos state as a case study area. The city is a fast growing where a significant proportion of the populace rely on borehole water supply. The study assessed the quality of the raw water samples and the effectiveness of the conventional treatment methods employed by most residents in the study area. The findings indicates that there are significant deviations in the various parameters of the raw water samples from the approved levels of the World Health Organization (WHO) and the Nigeria Standards for Drinking Water Quality (NSDWQ), showing the need for improved water treatment techniques. The use of Hypochlorite, Soda ash, Alum, and a three-stage filtration system was proposed and tested, yielding significant improvements in both the physicochemical and bacteriological parameters of the water samples when compared with the control standards. The research contributes to the growing body of knowledge targeted at improving water quality, especially in regions that depend heavily on borehole water supply. The findings emphasize the benefits of implementing comprehensive and efficient water treatment methos, including the need for continuous optimization and monitoring of these techniques. This is important for promoting public access to safe and wholesome potable water, thereby contributing to the achievement of Sustainable Development Goal 6 (Clean Water and Sanitation). Further research is recommended to explore the scalability and cost-effectiveness of the improved treatment method for wider application.