bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluid restrictive resuscitation with high molecular weight hyaluronan infusion in early peritonitis sepsis
Authors: Tenhunen Annelie Barrueta , van der Heijden Jaap , Skorup Paul , Maccarana Marco , Larsson Anders , Larsson Anders , Perchiazzi Gaetano , Tenhunen Jyrki 
Published: September 2023

AbstractSepsis is a condition with high morbidity and mortality. Prompt recognition and initiation of treatment is essential. Despite forming an integral part of sepsis management, fluid resuscitation may also lead to volume overload, which in turn is associated with increased mortality. The optimal fluid strategy in sepsis resuscitation is yet to be defined. Hyaluronan, an endogenous glycosaminoglycan with high affinity to water is an important constituent of the endothelial glycocalyx. We hypothesized that exogenously administered hyaluronan would counteract intravascular volume depletion and contribute to endothelial glycocalyx integrity in a fluid restrictive model of peritonitis. In a prospective, blinded model of porcine peritonitis sepsis, we randomized animals to intervention with hyaluronan (n = 8) or 0.9% saline (n = 8). The animals received an infusion of 0.1% hyaluronan 6 ml/kg/h, or the same volume of saline, during the first 2 h of peritonitis. Stroke volume variation and hemoconcentration were comparable in the two groups throughout the experiment. Cardiac output was higher in the intervention group during the infusion of hyaluronan (3.2 ± 0.5 l/min in intervention group vs 2.7 ± 0.2 l/min in the control group) (p = 0.039). The increase in lactate was more pronounced in the intervention group (3.2 ± 1.0 mmol/l in the intervention group and 1.7 ± 0.7 mmol/l in the control group) at the end of the experiment (p < 0.001). Concentrations of surrogate markers of glycocalyx damage; syndecan 1 (0.6 ± 0.2 ng/ml vs 0.5 ± 0.2 ng/ml, p = 0.292), heparan sulphate (1.23 ± 0.2 vs 1.4 ± 0.3 ng/ml, p = 0.211) and vascular adhesion protein 1 (7.0 ± 4.1 vs 8.2 ± 2.3 ng/ml, p = 0.492) were comparable in the two groups at the end of the experiment. In conclusion, hyaluronan did not counteract intravascular volume depletion in early peritonitis sepsis. However, this finding is hampered by the short observation period and a beneficial effect of HMW-HA in peritonitis sepsis cannot be discarded based on the results of the present study.
Integrating Deloading into Strength and Physique Sports Training Programmes: An International Delphi Consensus Approach
Authors: Bell Lee , Strafford Ben William , Coleman Max , Androulakis Korakakis Patroklos , Nolan David 
Published: September 2023

Abstract
Background
Deloading is a ubiquitous yet under-researched strategy within strength and physique training. How deloading should be integrated into the training programme to elicit optimal training outcomes is unknown. To aid its potential integration, this study established consensus around design principles for integrating deloading in strength and physique training programmes using expert opinion and practical experience.

Methods
Expert strength and physique coaches were invited to an online Delphi consisting of 3 rounds. Thirty-four coaches completed the first round, 29 completed the second round, and 21 completed the third round of a Delphi questionnaire. In the first round, coaches answered 15 open-ended questions from four categories: 1: General Perceptions of Deloading; 2: Potential Applications of Deloading; 3: Designing and Implementing Deloading; and 4: Creating an Inclusive Deloading Training Environment. First-round responses were analyzed using reflexive thematic analysis, resulting in 138 statements organized into four domains. In the second and third rounds, coaches rated each statement using a four-point Likert scale, and collective agreement or disagreement was calculated.

Results
Stability of consensus was achieved across specific aspects of the four categories. Findings from the final round were used to develop the design principles, which reflect the consensus achieved.

Conclusions
This study develops consensus on design principles for integrating deloading into strength and physique sports training programmes. A consensus definition is proposed: “Deloading is a period of reduced training stress designed to mitigate physiological and psychological fatigue, promote recovery, and enhance preparedness for subsequent training.” These findings contribute novel knowledge that might advance the current understanding of deloading in strength and physique sports.
Microbial community changes correlate with impaired host fitness of Aurelia aurita after environmental challenge
Authors: Pinnow Nicole , Chibani Cynthia M. , Güllert Simon , Weiland-Bräuer Nancy 
Published: September 2023

AbstractClimate change globally endangers certain marine species, but at the same time, such changes may promote species that can tolerate and adapt to varying environmental conditions. Such acclimatization can be accompanied or possibly even be enabled by a host’s microbiome; however, few studies have so far directly addressed this process. Here we show that acute, individual rises in seawater temperature and salinity to sub-lethal levels diminished host fitness of the benthic Aurelia aurita polyp, demonstrated by up to 34% reduced survival rate, shrinking of the animals, and almost halted asexual reproduction. Changes in the fitness of the polyps to environmental stressors coincided with microbiome changes, mainly within the phyla Proteobacteria and Bacteroidota. The absence of bacteria amplified these effects, pointing to the benefit of a balanced microbiota to cope with a changing environment. In a future ocean scenario, mimicked by a combined but milder rise of temperature and salinity, the fitness of polyps was severely less impaired, together with condition-specific changes in the microbiome composition. Our results show that the effects on host fitness correlate with the strength of environmental stress, while salt-conveyed thermotolerance might be involved. Further, a specific, balanced microbiome of A. aurita polyps supports the host’s acclimatization. Microbiomes may provide a means for acclimatization, and microbiome flexibility can be a fundamental strategy for marine animals to adapt to future ocean scenarios and maintain biodiversity and ecosystem functioning.
Mannose-anchored solid lipid nanoparticles loaded with atorvastatin calcium and vinpocetine as targeted therapy for breast cancer
Authors: Shinde Amol S. , Lala Rita R. 
Published: September 2023

Abstract
Background
This study was aimed to design mannose-conjugated solid lipid nanoparticles (MSLNs) for the targeted delivery of Atorvastatin Calcium (ATS) and Vinpocetine (VIN) to augment its therapeutic efficacy against breast cancer. SLNs were prepared by hot emulsification ultra-probe sonication method and conjugated with mannose. In vitro cell line, in vivo pharmacokinetic and in vivo tumor regression studies were performed for MSLNs.

Results
MSLNs had an average particle size of 435.4 ± 3 nm with polydispersity index 0.298 ± 0.03 and a zeta potential of − 28.2 ± 1 emv. Entrapment efficiency was found to be 69.17 ± 0.92%, 71.18 ± 0.68% for ATS and VIN, respectively. The IC50 value of MSLNs was 1.46 µg/ml, which is efficient to control the growth of MDA MB231 cells as compared to the individual drugs and combinatorial SLNs. The combination index was found to be 0.7. MSLNs inhibited cell growth via necrosis by promoting to apoptosis through arresting SubG1 phase. The relative bioavailability of ATS and VIN loaded in MSLNs was 1.47 and 5.70, respectively, as compared to the marketed formulation. Maximal tumor volume reduction and higher survival rate was found for the MSLNs group (76.03%, P = 0.0001) as compared to the control group (P = 0.0364), individual drugs SLNs group.

Conclusion
The results revealed that the MSLNs formulation augmented activity against breast cancer by inhibiting the cell growth. This promising drug delivery reduces the doses for both the drugs and attains minimal dose-associated side effects with synergism by reaching the specific target site, furthermore improving the therapeutic efficacy.
Mutual Fund Fragility, Dealer Liquidity Provision, and the Pricing of Municipal Bonds
Authors: Li Yi , O’Hara Maureen , Zhou Xing (Alex) 
Published: September 2023

Against the backdrop of COVID-19, we study how the interactions of mutual funds and dealers introduce fragility to the municipal bond market and induce lasting market impacts. During the crisis, trading surges, whereas dealers’ liquidity provision plunges for mutual-fund-held bonds, leading to greater price depressions in these bonds. Importantly, the crisis reshapes the market’s perceptions of mutual fund fragility risks, with the aftermath-yield spreads widening significantly more for bonds with greater mutual fund exposures. Such postcrisis pricing effects reflect dealers’ continued reluctance to provide liquidity for mutual fund–held bonds, and they are stronger for bonds whose mutual fund holders are more susceptible to investor runs. This paper was accepted by Victoria Ivashina, finance. Supplemental Material: The data files are available at https://doi.org/10.1287/mnsc.2023.4912 .
Glucose fluctuation promotes mitochondrial dysfunctions in the cardiomyocyte cell line HL-1
Authors: Mordel Patrick , Fontaine Fanny , Dupas Quentin , Joubert Michael , Allouche Stéphane 
Published: September 2023

Aims
Glycemic variability has been suggested as a risk factor for diabetes complications but the precise deleterious mechanisms remain poorly understood. Since mitochondria are the main source of energy in heart and cardiovascular diseases remain the first cause of death in patients with diabetes, the aim of the study was to evaluate the impact of glucose swings on mitochondrial functions in the cardiomyocyte cell line HL-1.

Methods
HL-1 cells were exposed to low (LG, 2.8 mmol/l), normal (NG, 5.5 mmol/l), high (HG, 25 mmol/l) or intermittent high glucose (IHG, swing between low and high) every 2h during 12h (short-time treatment) or every 12h during 72h (long-time treatment). Anaerobic catabolism of glucose was evaluated by measuring glucose consumption and lactate production, oxidative phosphorylation was evaluated by polarography and ATP measurement, mitochondrial superoxide anions and the mitochondrial membrane potential (MMP) were analysed using fluorescent probes, and the protein oxidation was measured by oxyblot.

Results
IHG and HG increased glucose consumption and lactate production compared to LG and NG but without any difference between short- and long-time treatments. After 72h and unlike to LG, NG and HG, we didn’t observe any increase of the mitochondrial respiration in the presence of succinate upon IHG treatment. IHG, and to a lesser extent HG, promoted a time-dependent decrease of the mitochondrial membrane potential compared to LG and NG treatments. HG and IHG also increased superoxide anion production compared to LG and NG both at 12 and 72h but with a higher increase for IHG at 72h. At last, both HG and IHG stimulated protein oxidation at 72h compared to LG and NG treatments.

Conclusions
Our results demonstrated that exposure of HL-1 cells to glucose swings promoted time-dependent mitochondrial dysfunctions suggesting a deleterious effect of such condition in patients with diabetes that could contribute to diabetic cardiomyopathy.
Prediction of outpatients with conjunctivitis in Xinjiang based on LSTM and GRU models
Authors: Wang Yijia , Yi Xianglong , Luo Mei , Wang Zhe , Qin Long , Hu Xijian , Wang Kai 
Published: September 2023

Background
Reasonable and accurate forecasting of outpatient visits helps hospital managers optimize the allocation of medical resources, facilitates fine hospital management, and is of great significance in improving hospital efficiency and treatment capacity.

Methods
Based on conjunctivitis outpatient data from the First Affiliated Hospital of Xinjiang Medical University Ophthalmology from 2017/1/1 to 2019/12/31, this paper built and evaluated Long Short-Term Memory (LSTM) and Gated Recurrent Unit (GRU) models for outpatient visits prediction.

Results
In predicting the number of conjunctivitis visits over the next 31 days, the LSTM model had a root mean square error (RMSE) of 2.86 and a mean absolute error (MAE) of 2.39, the GRU model has an RMSE of 2.60 and an MAE of 1.99.

Conclusions
The GRU method can better predict trends in hospital outpatient flow over time, thus providing decision support for medical staff and outpatient management.
Auditory function in humans at high altitude. A scoping review
Authors: Masè Michela , Viziano Andrea , Strapazzon Giacomo , Alessandrini Marco , Micarelli Alessandro 
Published: September 2023

Purpose
High-altitude (HA) affects sensory organ response, but its effects on the inner ear are not fully understood. The present scoping review aimed to collect the available evidence about HA effects on the inner ear with focus on auditory function.

Methods
The scoping review was conducted following the guidelines of the Preferred Reporting Items for Systematic Review and Meta-Analysis extension for scoping reviews. PubMed, Scopus, and Web of Science electronic databases were systematically searched to identify studies conducted in the last 20 years, which quantified in healthy subjects the effects of HA on auditory function.

Results
The systematic search identified 17 studies on a total population of 888 subjects (88.7% male, age: 27.8 ± 4.1 years; median sample size of 15 subjects). Nine studies were conducted in a simulated environment and eight during real expeditions at HA. To quantify auditory function, six studies performed pure tone audiometry, four studies measured otoacoustic emissions (OAE) and eight studies measured auditory evoked responses (AER). Study protocols presented heterogeneity in the spatio-temporal patterns of HA exposure, with highly varying maximal altitudes and exposure durations.

Conclusion
Most studies reported a reduction of auditory function with HA in terms of either elevation of auditory thresholds, lengthening of AER latencies, reduction of distortion-product and transient-evoked OAEs. Future studies in larger populations, using standardized protocols and multi-technique auditory function evaluation, are needed to further characterize the spatio-temporal pattern of HA effects along the auditory pathways and clarify the pathophysiological implications and reversibility of the observed changes.
Determinants of institutional delivery service utilization in Nepal
Authors: Thapa Bipin , Karki Anita , Sapkota Suman , Hu Yifei 
Published: September 2023

Background
Maternal mortality continues to be a pressing concern in global health, presenting an enduring and unmet challenge for healthcare systems worldwide. Utilization of institutional delivery services has been established as a proven intervention to mitigate life-threatening risks for both mothers and newborns. Exploring the determinants of institutional delivery is crucial to improve and enhance maternal and newborn safety. This study aimed to assess the contextual and individual factors associated with institutional delivery in Nepal.

Methods
This study utilized that data form Nepal Multiple Indicator Survey 2019, which included a sample of 1,932 women who had given birth within the two years prior to the survey. A multilevel logistic regression analysis was performed to determine the significant external environment, contextual and individual predictors of institutional delivery.

Results
The women from Madhesh province [Adjusted Odds Ratio (aOR): 0.32, 95% Confidence Interval (CI): 0.17–0.61], as compared to Bagmati province, women from rural areas (aOR: 0.55, 95% CI: 0.39–0.78) as compared to urban areas, and women from a relatively less-advantaged ethnic groups (aOR: 0.52, 95% CI: 0.35–0.76) as compared to the relatively advantaged ethnic groups were less likely to deliver in health institutions. Similarly, women from the poorest (aOR: 0.09, 95% CI: 0.04–0.22) and second wealth groups (aOR: 0.29, 95% CI: 0.13–0.64) were less likely to attend institute for delivery compared to women from the richest household. Women with formal education (aOR: 1.65, 95% CI: 1.16–2.35) were more likely to deliver in an institution over uneducated women. Moreover, the uptake of institutional delivery increased by 59% (aOR: 1.59, 95% CI: 1.43–1.75) for each additional ANC visit.

Conclusion
The findings highlight the importance of stepping up efforts to achieve universal health care from the standpoint of long-term government investment, focusing particularly on illiterate women in rural areas, poorer households, and socially disadvantaged groups. Expanding the benefits of maternal benefit schemes targeting the women from the poorest households in the communities is recommended.
Prediction of genetic gains in sweet potato genotypes by polycross
Authors: Mariano Giovana Guerra , Pavan Bruno Ettore , Otoboni Maria Eduarda Facioli , Andrade Maria  Isabel , Vargas Pablo Forlan 
Published: September 2023

Sweet potato crops present a wide genetic diversity, but genetic parameters of populations should be estimated in breeding programs, mainly when the selection is focused on multiple traits. Thus, the objective of this study was to estimate genetic parameters and select clones via a selection index in a sweet potato population obtained from uncontrolled crosses. The experiment was carried out in Ilha Solteira, Sao Paulo, Brazil, from June to November 2020, to evaluate 144 clones and three controls, totaling 147 treatments. A randomized block design was used, with two replications and three plants per plot. The data were analyzed through mixed modeling (REML/BLUP), and the selection index proposed by Mulamba & Mock was applied using a selection pressure of 17.3% for the selection of superior clones. The direct selection enabled a higher prediction of gains for each trait, but the joint analysis maximized the selection gains for all traits of interest. Clones 38, 63, 79, 77, and 5 are recommended, among the selected clones, for further stages of sweet potato breeding focused on improving traits for production and table consumption.

Palavras-chave: Ipomoea batatas (L.) Lam, Variabilidade genética, Índice de seleção, Parâmetros genéticos, Ganho com seleção
Managing and controlling diffuse urbanisation in France: Spatial planning practices in cross-border city-regions
Authors: Le Bivic Camille , Idt Joël 
Published: September 2023

Abstract
Managing and controlling “land take” of agricultural and natural land for urbanisation is the target of European and national policies. Although this objective seems to be increasingly understood among the actors, the prioritisation of issues and the measures taken by governments to achieve it are very heterogeneous. The practices of local public and private actors do not necessarily follow the directions established at national level by the instruments. This paper examines the concrete forms, in France, of collective action in the field of spatial planning to limit land take, particularly in French cross-border areas that are experiencing strong urban growth. The survey is based on 60 semi-structured interviews with public actors involved in the spatial transformations of the cross-border regions around Geneva, Lille-Kortrijk-Tournai, Luxembourg and Strasbourg-Karlsruhe. The survey shows that collective action in spatial planning is very diverse in France, depending on the regions and municipalities concerned, even if the legal rules are the same. Local public actors play strategically with the rules and adapt them. Moreover, the organisation of collective action to limit land take is marked by interdependencies between regions, and in our case between neighbouring countries, which are often left unexamined in national planning policies and instruments.
Ewolucja egzaminu maturalnego z języków obcych na przestrzeni sześciu dekad
Authors: Sendur Agnieszka M. 
Published: September 2023

Matura is an important high-stakes examination present in the Polish education system since the late 18th century. It is taken on completion of high school and is compulsory for those who want to apply for higher education. As a result of an examination reform, in 2005 the exam took on a new form. Since then it has been a professional, external, standardised assessment used for the analysis of school and student performance, and – most importantly – in lieu of university entrance exams.
The article looks at the evolution of the foreign language component of the Matura exam over the last six decades: from an internal test prepared by local authorities and by schools, assessed arbitrarily by the test-takers’ teachers, to a professional examination prepared by expert institutions in compliance with quality standards and assessed by trained markers according to elaborate grading criteria. The paper follows the changes occurring in the assessment starting from 1960 up to 2023, when another examination reform brought about new modifications in the foreign language exam.
Recurrent genetic variants and prioritization of variants of uncertain clinical significance associated with hereditary breast and ovarian cancer in families from the Region of Murcia
Authors: Rosado-Jiménez Laura , Mestre-Terkemani Younes , García-Aliaga Ángeles , Marín-Vera Miguel , Macías-Cerrolaza José Antonio , Sarabia-Meseguer María Desamparados , García-Hernández María Rosario , Zafra-Poves Marta , Sánchez-Henarejos Pilar , Ayala de la Peña Francisco , Alonso-Romero José Luis , Noguera-Velasco José Antonio , Ruiz-Espejo Francisco 
Published: September 2023

Abstract

Objectives
Hereditary breast and ovarian cancer (HBOC) follows an autosomal dominant inheritance pattern of cancer susceptibility genes. The risk of developing this disease is primarily associated with germline mutations in the BRCA1 and BRCA2 genes. The advent of massive genetic sequencing technologies has expanded the mutational spectrum of this hereditary syndrome, thereby increasing the number of variants of uncertain clinical significance (VUS) detected by genetic testing.

Methods
A prevalence study of HBOC was performed within 2,928 families from the Region of Murcia, in southeastern Spain. Genetic testing enabled the identification of recurrent pathogenic variants and founder mutations, which were mainly related to the BRCA1 and BRCA2 genes. VUS testing was performed using a prioritization algorithm designed by our working group.

Results
Variants c.68_69del, c.212+1G>A, and c.5123C>A were detected in 30 % of BRCA1 carriers, whereas exon 2 deletion concurrent with c.3264dupT, c.3455T>G and c.9117G>A variants were found in 30 % of BRCA2 carriers. A total of 16 VUS (15 %) were prioritized.

Conclusions
The genotype-phenotype correlation observed in our study is consistent with the scientific literature. Furthermore, the founder effect of c.1918C>T (BRCA1) and c.8251_8254del (ATM) was verified in the Murcian population, whereas exon 2 deletion (BRCA2) was proven to be a Spanish founder mutation. Our algorithm enabled us to prioritize potentially pathogenic VUS that required further testing to determine their clinical significance and potential role in HBOC.
A Note on a Moment Inequality
Authors: Jeong Seungwon (Eugene) 
Published: September 2023

Abstract
Using Chebyshev’s sum inequality, we generalize E[X
2] ≥ E
2[X] into a moment inequality with a general combination of moments that compares E[X

r+s
] and E[X

r
]E[X

s
]. We also provide its economic application.