bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep-Learning-Based Survival Prediction of Patients with lower limb melanoma
Authors: Zhang Jinrong , Yu Hai , Zheng Xinkai , Ming Wai-kit , YAU SUN LAK , Kong Ching Tom , Lee Alice , Huang Hui , Chen Wenhui , Lyu Jun , Deng Liehua 
Published: August 2023

Abstract
Background
For the purpose to examine lower limb melanoma (LLM) and its long-term survival rate, we used data from the Surveillance, Epidemiology and End Results (SEER) database. To estimate the prognosis of LLM patients and assess its efficacy, we used a powerful deep learning and neural network approach called DeepSurv.
Methods:
We gathered data on those who had an LLM diagnosis between 2000 and 2019 from the SEER database. We divided the people into training and testing cohorts at a 7:3 ratio using a random selection technique. To assess the likelihood that LLM patients would survive, we compared the results of the DeepSurv model with those of the Cox proportional-hazards (CoxPH) model. Calibration curves, the time-dependent area under the receiver operating characteristic curve (AUC), and the concordance index (C-index) were all used to assess how accurate the predictions were.
Results:
In this study, a total of 26,243 LLM patients were enrolled, with 7,873 serving as the testing cohort and 18,370 as the training cohort. Significant correlations with age, gender, AJCC stage, chemotherapy status, surgery status, regional lymph node removal and the survival outcomes of LLM patients were found by the CoxPH model. The CoxPH model's C-index was 0.766, which signifies a good degree of predicted accuracy. Additionally, we created the DeepSurv model using the training cohort data, which had a higher C-index of 0.852. In addition to calculating the 3-, 5-, and 8-year AUC values, the predictive performance of both models was evaluated. The equivalent AUC values for the CoxPH model were 0.795, 0.767, and 0.847, respectively. The DeepSurv model, in comparison, had better AUC values of 0.872, 0.858, and 0.847. In comparison to the CoxPH model, the DeepSurv model demonstrated greater prediction performance for LLM patients, as shown by the AUC values and the calibration curve.
Conclusion:
We created the DeepSurv model using LLM patient data from the SEER database, which performed better than the CoxPH model in predicting the survival time of LLM patients.
Synthesis, Characterization, and Antibacterial Activity Studies of two Co(II) Complexes with 2-[(E)-(3-acetyl-4-hydroxyphenyl)diazenyl]-4-(2-hydroxyphenyl)thiophene-3-carboxylic acid as a Ligand
Authors: Fondjo Emmanuel Sopbué , Feuze Sorelle Songmi , Tamokou Jean-de-Dieu , Tsopmo Apollinaire , Doungmo Giscard , W. Peter Simon F. , Tsamo Donald Léonel Feugap , Ndjakou Bruno Lenta , Kuiate Jules Roger 
Published: August 2023

Abstract
Two new Cobalt(II) complexes 5 and 6 with 2-[(E)-(3-acetyl-4-hydroxyphenyl)diazenyl]-4-(2-hydroxyphenyl)thiophene-3-carboxylic acid 4 as a novel ligand have been synthesized. These three new compounds were characterized on the basis of their powder X-Ray Diffraction, UV-Vis, IR, NMR, elemental analysis and MS spectral data. DFT/B3LYP mode of calculations were carried out to determine some theorical parameters of the molecular structure of the ligand. The purity of the ligand and the metal complexes were ascertained by TLC and melting points. The analysis of the IR spectra of the polyfunctionalized azo compound 4 and its metal complexes 5 and 6, reveals that the coordination patterns of the ligand are hexadentate and tetradentate respectively. Based on the UV-Vis electronic spectral data, octahedral and square-planar geometries were respectively assigned to the cobalt(II) complexes. The ligand 4 displayed moderate antibacterial activity (MIC = 32–128 µg/mL) against Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Pseudomonas aeruginosa and Klebsiella pneumoniae 22. The octahedral Cobalt(II) complex 5 showeds moderate activity against Pseudomonas aeruginosa (MIC = 128 µg/mL) and Klebsiella pneumoniae 22 (MIC = 64 µg/mL) and none against Staphylococcus aureus ATCC25923 and Escherichia coli ATCC25922, whereas the square-planar complex 6 displayed moderate activity only on Klebsiella pneumoniae 22 (MIC = 64 µg/mL).
Natural History of the Pelvic Binder or “Sheet-wrap” in the Early Treatment of Patients with Life-threatening Pelvic Ring Injuries at a Busy Level One Regional Trauma Center. A Retrospective Study.
Authors: Rodriguez-Buitrago Andres , Collinge Cory , Guillamondegui Oscar , Dennis Bradley , Bream Peter , Mitchell Phillip M. 
Published: August 2023

Abstract
Objective:Pelvic binders and “sheet-wraps” (PB/SW) are critical tools in splinting the injured pelvis of severely injured trauma patients. Our study reviews the management, logistics, and documentation of PB/SW use in a busy academic Level 1 Trauma Center.
Methods: Adults patients with pelvic ring injury from high energy trauma presenting with shock between July 1, 2015 and June 31, 2017 requiring the use of a PB/SW were included in the study, a retrospective review of the medical records was performed evaluating when, how, and by whom PB/SW’s were applied, removed, or manipulated during early hospitalization.
Results:Documentation and management of PB/SW’s werewell below standard benchmarks of other procedures, tools, and interventions used in acutely ill patients. No information was documented about PB/SW application in 44% of the 36 cases when the PB/SW was applied at our center. Alterations to the PB/SW were ineffectually communicated and coordinated between members of the treatment team with all PB/SW removed from 21 of patients treated with an emergency procedure in interventional radiology (15) or the operating room (6).
Conclusions:Our use and documentation of PB/SW’s in the early treatment of trauma patients with pelvic injuries in shock appear lacking. Immediate action will be taken to improve on these benchmarks through process improvement with caregiver education, improved communication, and creation of measurable benchmarks.
Level of Evidence: Level IV. See Instructions for Authors for a complete descriptionof levels of evidence.
Inflammatory biomarkers may be associated with poor outcomes after mechanical thrombectomy
Authors: Wang Hong , Tian Xiaobing , Liao Zhangyuan , Yue Xuanye , Sun Libin , Li Xingrong , Zou Ming , Ding Jiayue 
Published: August 2023

Abstract

Background:Mechanical thrombectomy (MT) has become the mainstay of treatment for acute ischemic stroke (AIS) in recent years. This case-control study aimed to identify the pivotal role of inflammation in the prognosis of AIS patients after MT.Methods:Altogether, 70 AIS patients who underwent MT were retrospectively recruited for this study. ROC analysis was performed to demonstrate the sensitivity and specificity of the inflammatory variables for predicting prognosis. A meta-analysis was further performed to pool the published results together. Stata software was used for analysis in this study.Results:A total of 70 patients were enrolled in this study. There was no differences in pre-MT inflammatory biomarkers between patients who survived and those who died, as well as patients with mRS 0–2 and mRS ≥ 3. In contrast, post-MT CRP levels might be a potential parameter to predict death after thrombectomy (AUC, 95%CI, 0.737, 0.587-0.887; p=0.005; optimal cutoff value=4.565). Moreover, post-MT monocyte count might be an appropriate parameter to predict poor long-term prognosis after thrombectomy (AUC, 95%CI, 0.704, 0.575-0.833; p=0.017; optimal cutoff value=0.345). A meta-analysis revealed that the pre-MT inflammatory indices, including WBC count (WMD, 95%CI, 1.32, 1.01—1.63), neutrophil count (1.23, 0.95—1.51), monocyte count (0.05, 0.02—0.09), NLR (2.42, 1.98—2.87) and PLR (24.65, 7.99—41.32), were higher in patients with 3-month mRS ≥ 3, and the lymphocyte count (-0.28, -0.40—-0.16) was lower in this cohort.Conclusions:Inflammatory indices were significantly associated with the prognosis of patients undergoing MT, especially post-MT CRP and monocyte count, which can predict long-term outcomes.
The impact of the length of proximal margin on the prognosis of adenocarcinoma of gastroesophageal junction and coping strategies: A real-world study
Authors: Pang Tao , Qin Ying Yi , Nie MingMing , Fan Lei , Wang Jia Yang , Liu Zhao Rui , Yin Kai 
Published: August 2023

Abstract

Background: The optimal proximal margin (PM) length for SiewertII/III adenocarcinoma of the esophagogastric junction (AEJ) remains unclear. This study aimed to determine the optimal PM length using an abdominal approach to guide surgical decision-making.
Methods: A prospective study analyzed 304 consecutive patients diagnosed with SiewertII/III AEJ between January 2019 and December 2021. Total gastrectomy was performed via the abdominal approach, and PM length was measured on fixed gross specimens. X-Tile software determined the optimal PM cut-point based on progression-free survival (PFS). Univariate analyses compared baseline characteristics across PM groups, while survival analyses utilized Kaplan-Meier estimation and Cox proportional hazards regression for assessing the impact of margin length on survival. Multivariable analyses were conducted to adjust for confounding variables.
Results: The study included 264 AEJ cases classified as Siewert II (71.97%) or III (28.03%). The median gross PM length was 1.0 cm (IQR: 0.5 cm-1.5 cm, range: 0 cm-6 cm). PM length ≥1.2 cm was associated with a lower risk of disease progression compared to PM length 0.4 cm on PFS (HR = 0.41, 95% CI 0.20-0.84, P = 0.015). Moreover, PM ≥1.2 cm improved prognosis in subgroups of T4 or N3, tumor size <4 cm, Siewert II, and Lauren classification.
Conclusions: For Siewert type II/III AEJ, a proximal margin length ≥1.2 cm (1.65 cm in situ) is associated with improved outcomes. These findings offer valuable insights into the association between PM length and outcomes in SiewertII/III AEJ, providing guidance for surgical approaches and aiding clinical decision-making to enhance patient outcomes.
Investigating the relationship between health literacy and preconceptual care components in pregnancy: A Cross Sectional Study
Authors: Sajjadian Fatemeh , Amiri-Farahani Leila , Haghani Shima , Pezaro Sally 
Published: August 2023

Abstract
Background and Aim: Preconceptual care aiming to improve health is influenced by various factors including health literacy. Considering the importance and necessity of high quality preconceptual care, this study aimed to determine the relationship between health literacy and receiving components of preconceptual care prior to pregnancy.
Methods: This cross-sectional study included 693 participants with pregnancies of less than 14 weeks gestation referred to health centers and gynecologists in Shiraz city, Iran. Multi-stage sampling was done from May 2021 to February 2022 in 18 comprehensive urban health centers and 20 gynecology offices via proportional allocation method. The data collection tool comprised a questionnaire consisting of 3 parts: 1) individual and fertility characteristics, 2) information related to the components of preconceptual care and 3) health literacy for Iranian adults. This was completed by individual participants via the self-reporting method.
Results: The majority of participants in the study were between 30 and 34 years old. They were also women with a university education, predominantly working as housewives. The mean health literacy of participants was 76.81%. Health literacy obtained the highest mean score in the dimension of ‘understanding’ and the lowest mean score in the dimension of ‘access’. The frequency of preconceptual counseling, folic acid supplement consumption, exercise, blood testing, dental visits, genetic counseling, Pap smear testing and rubella, diphtheria, and hepatitis vaccinations prior to pregnancy was 66.8%, 53.8%, 45.6%, 71.86%, 44.44%, 12%, 53.4%, 10.83%, respectively. Many (> 64%) received preconceptual care at specialist gynecology offices. Results demonstrated that health literacy had a statistically significant relationship with preconceptual care, folic acid consumption, exercise and dental care, (p < 0.001), along with blood testing and Pap smear testing (p < 0.05).
Conclusion: Overall, our results demonstrate that despite health literacy being optimal, uptakes of some components of PCC are low. As such, it will be important to further raise awareness of the importance of PCC for people prior to pregnancy as a priority in health promotion and education.
Combined laparoscopic pyeloplasty and suctioning ureteral access sheath assisted flexible ureteroscopy in the treatment of ureteropelvic junction obstruction coexisting with renal calculi: A multi-center retrospective observational study
Authors: mi yang , Kang Zhiqin , Wang Jingyu , Yan Liang , zhang jun 
Published: August 2023

Abstract

Background Ureteropelvic junction obstruction (UPJO) is a common obstructive disease of the urinary tract. It is very common for UPJO patients to have coexistent renal calculi. The main aim of therapy is the relief of the obstruction and the removal of the stones at the same time. Methods This retrospective study included 110 patients diagnosed with the UPJO coexisting with multiple renal calculi at the Shanxi Bethune Hospital and the First Hospital of Shanxi Medical University between March 2016 and January 2022. Patients were divided according to the different methods of dealing with UPJO and renal calculi. In group A, patients received traditional open pyeloplasty and pyelolithotomy. In group B, patients received percutaneous nephrolithotomy first and then received laparoscopic pyeloplasty. In group C, patients received flexible cystoscopy to extract stones and then laparoscopic pyeloplasty. In group D, patients received vacuum suction access sheath-assisted flexible ureteroscopy and underwent laparoscopic pyeloplasty. The stones were smashed using a holmium laser. The success rate of pyeloplasty, stone clearance rate, operation time, bleeding amount, complication occurrence rate, post-surgical pain, length of stay, and hospitalization cost were compared between groups. The follow-up period was at least 2 years. Results By using flexible ureteroscopy and a vacuum suction urethral access sheath, the renal stone clearance rate was significantly increased, and the complication occurrence rate and the operation time were significantly reduced in the UPJO patients coexisting with multiple renal calculi. Conclusions Laparoscopic pyeloplasty combined with flexible ureteroscopy and a vacuum suction urethral access sheath is safe and effective in dealing with UPJO in patients complicated by renal caliceal stones. Trial registration Retrospectively registered
Gut microbiota alterations are associated with functional outcomes in patients of acute ischemic stroke with non-alcoholic fatty liver disease
Authors: Yu Gaojie , Chen Qionglei , Chen Jiaxin , Liao Xiaolan , Xie Huijia , Zhao Yiting , Liu Jiaming , Sun Jing , Chen Songfang 
Published: August 2023

Abstract
Background
Non-alcoholic fatty liver disease (NAFLD) is a risk factor for adverse prognosis of acute ischemic stroke (AIS) and a common comorbidity in patients with AIS. However, the mechanisms by which NAFLD increases the risk of the adverse prognosis remain unknown. This study explored the changes in gut microbiota of AIS patients with NAFLD to expand the understandings of their mechanisms from the perspective of gut microbiota.
Methods
Patients were recruited and divided into 2 groups comprising 64 patients with AIS and NAFLD and 85 patients with AIS but without NAFLD. The stool samples and clinical information were collected. 16s rRNA sequencing was used to analyze the composition of gut microbiota. The patients were followed-up at 3 months post-stroke. The adverse outcome was assessed by modified Rankin Scale. The changes in microbial composition and key bacteria were assessed, and potential microbial marker for predicting risks for the adverse prognosis in AIS patients with NAFLD based on receiver operating characteristic curve was identified.
Results
The relative abundance of Dorea, Dialister, Intestinibacter, Flavonifractor significantly decreased in NAFLD group, while the relative abundance of Enorma significantly increased in NAFLD group. Dorea was identified as the potential microbial marker for predicting the risk of adverse prognosis in AIS patients with NAFLD (area under the curve > 0.600).
Conclusions
This study demonstrated comprehensively gut microbiota characteristics in AIS patients with NAFLD, screened out crucial microbe related to adverse prognosis, and identified the potential microbial marker for predicting the risk of AIS patients with NAFLD.
Molecular regulation of volatile organic compounds accumulation in tomato leaf by different nitrogen treatments
Authors: gao hongdou , Xu ru , Du zhijie , Ye Huilan , Tian Jun , Huang Weiqun , Xu Shuiyong , Xu Fule , Hou Maomao , Zhong Fenglin 
Published: August 2023

Abstract

Tomato plants are easily infected by pests and fungi, which seriously influences the yield and quality. The objective of the present study was to determine the effect of five types of nitrogen (N) treatments on volatile organic compounds (VOCs) by determining the changes in the transcriptome levels of genes related to phenylalanine metabolism, amino acid biosynthesis and plant-pathogen interaction as well as VOCcontent in tomato leaf during growth. The results indicated that low N treatment (1/20 N and 1/5 N) promoted the production of VOCs compared to high N treatment (2 N and 3 N). Low N treatment enhanced the levels of α-pinene, camphene, β-ocimene, D-limonene, caryophyllene, humulene, terpineol, carveol, (E)-2-hexanal, methylis salicylas and cyclobutene. Low N treatment upregulated the differentially expressed genes (DEGs) involved in phenylalanine metabolism and amino acid biosynthesis, which may influence peroxidase, 4-coumarate-CoA ligase, 4-hydroxyphenylpyruvate dioxygenase, caffeoyl-CoA O-methyltransferase, tyrosine aminotransferase, glycine hydroxymethyltransferase, anthranilate phosphoribosyltransferase, branched-chain amino acid aminotransferase, pyruvate kinase (PK), fructose-bisphosphate aldolase, and other enzymes. These enzymes could increase the synthesis of VOCs. Additonally, the DEGs in plant-pathogen interaction have a significant down-regulated trend in 2 N and 3 N compared with 1/20 N, which also responsed the low N could enhance the defense capacity in tomato. In summary, Transcriptome data showed that low N could promote the up-regulation of volatile substances related genes and the increase of VOCs verified the above changes in transcriptome data. Meanwhile, the change of gene in plant-pathogen interaction also demonstrate low N can enhance the defense capacity during tomato growth.
Low CDKN1B expression accompanied by reduced tumor-infiltrating lymphocytes is correlated with an adverse prognosis in breast cancer: machine learning analysis and drug discovery
Authors: Kim Hyung Suk , Noh Yung-Kyun , Min Kyueng-Whan , Kim Dong-Hoon , Son Byoung Kwan , Jung Un Suk , Kwon Mi Jung , Kim Young Seo , Oh Ki-Wook , Pyo Jung Soo 
Published: August 2023

Abstract
The cyclin-dependent kinase inhibitor 1B (CDKN1B) gene encodes the p27Kip1 protein, which plays an important role in cell cycle regulation and cell proliferation, and its role in breast cancer prognosis is controversial. The aim of this study was to analyze the clinicopathological parameters, molecular interactions, and anticancer immune responses in patients with CDKN1B expression. We investigated the clinicopathologic parameters, survival rates, proportions of immune cells, gene sets and prognostic models according to CDKN1B expression in 3,149 breast cancer patients. We performed gene set enrichment analysis (GSEA), in silico cytometry, pathway network analyses, in vitro drug screening and gradient boosting machine (GBM) learning. High CDKN1B expression levels in breast cancer correlated with high lymphocyte infiltration signature scores and increased CD8 + T cells, which were associated with a better prognosis. CDKN1B expression was associated with gene sets for upregulation of T-cell receptor signaling pathways and downregulation of CD8 + T cells. Pathway network analysis showed that CDKN1B was linked directly to positive regulation of the protein catabolic process pathway and indirectly to the T-cell receptor signaling pathway. We found that BMS-345541 was an effective drug that targeted CDKN1B and effectively supressed the growth of breast cancer cells with low CDKN1B expression in drug screening. The GBM model that included CDKN1B expression exhibited improved performance in predicting survival. CDKN1B expression may play an important role in the progression of breast cancer, and targeting CDKN1B may be a potential strategy for the treatment of breast cancer.
Efficacy and safety of stereotactic ablative brachytherapy (SABT) as salvage treatment for sacral region local recurrent rectal cancer: a multicenter retrospective study
Authors: Li Xuemin , Wang Zhe , Xing Chao , Wu Lirong , Wang Hao , Jiang Yuliang , Ji Zhe , Qiu Bin , Sun Haitao , Zhang Kaixian , Wang Ruoyu , Wang Junjie 
Published: August 2023

Abstract

Background:
To investigate the efficacy and safety of stereotactic ablative brachytherapy (SABT) as salvage treatment for sacral region local recurrent rectal cancer (S-LCCR).
Materials and Methods:
Fifty-two patients with S-LCCR after radical resection from January 2016 to December 2020 in four institutes were enrolled in this retrospective study. All patients received 3D-template (3D-PT) assisted SABT. Median tumor volume was 43.2 (range 3.1-178.7) cc. Local control (LC), overall survival (OS), pain relief, and adverse events was recorded and analyzed.
Results:
Median follow-up was 29.3 (range 15.7-42.9) months. Median LC was 24.0 (range 13.5-34.5) months. LC at 1-, 2- and 3-year were 66.7%, 46.9% and 39.1%, respectively. Median OS was 17.7 (range 9.6-25.9) months. OS at 1-, 2- and 3-year were 61.1%, 35.3% and 29.5%, respectively. Univariate analysis suggested that D90＞130Gy (P=0.007) and tumor volume ≤45cc (P=0.007) were positive prognostic factors influencing LC. Pain relief rate was 96.6% (28/29). Rate of adverse events (≥grade 3) was 7.7%.
Conclusion:
SABT may be a feasible and promising salvage treatment for sacral region recurrent rectal cancer after surgery.
Brown blotted root rot of ginger (Zingiber officinale) caused by Musidium stromaticum
Authors: Hayashi Kazusa , Hirooka Yuuri , Oki Tomoka , Shimomoto Yoshifumi , Yano Kazutaka 
Published: August 2023

Abstract
Brown lesions were found on ginger rhizomes in Kochi Prefecture, Japan, leading to the suspicion of an unknown disease. The filamentous fungus isolated at high frequency from the lesions replicated the disease symptoms upon inoculation, confirming it as the causal agent. Through morphological and molecular analyses, this filamentous fungus was identified as Musidium stromaticum. We propose the name “brown blotted root rot” to describe this new disease of ginger. This study is the first to recognized M. stromaticum as a plant pathogen of ginger globally.