bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Transfusion Support in Dengue Epidemic in Military Setting of Sri Lanka
Authors: Kumarage Samantha , Jayamanna Roshan , Mahendra Isanka 
Published: August 2023

Background:
Severe dengue affects most Asian and Latin American countries and becomes a leading cause of hospitalization and death among children and adults in these regions. During the year 2017, a total of 186,101 suspected dengue cases were reported, and as of May 24, 2018, a total of 19,459 suspected dengue cases were reported to the Ministry of Health of Sri Lanka with over 320 deaths in 2017 and 202 deaths in 2018. The aim of the study was to evaluate whether the blood and component transfusion for the dengue patients and the routine inventory management at our blood bank were affected by the nonfollowed transfusion guidelines in dengue management of Sri Lanka.

Methods:
This retrospective descriptive study analyzed the distribution of relative frequencies of variables in the requests received and tangible transfusions done from January 1, 2017, to December 31, 2017.

Results:
A total of 717 transfusion requests received from the confirmed dengue patients in 2017. In this cohort, 73 requests were for dengue fever, 494 requests for dengue hemorrhagic fever (DHF), 97 requests for DHF leaking phase, 46 requests for DHF critical phase, and 7 requests for dengue shock syndrome.

Conclusions:
Even though 60% of the actual transfusion requests followed the guidelines of the dengue management, 40% (13 requests) violated the guidelines in the actual transfusions. However, compared to the 717, total requests for transfusions this number is very minimal (0.97%). Of 3601 inventories managed at blood bank during 2017, additional dengue-related 717 requests, less than 1% violated the guidelines of transfusion requests according to the current guidelines of dengue management. No extra burden was created to inventory management due to nonfollowed guidelines. An unusual preponderance of transfusion requirements of AB patients was highlighted. Comparing the published data, dengue management at our hospital is exemplary.
Green Synthesis of Silver Nanoparticles using Phyllanthus amarus Seeds and their Antibacterial Activity Assessment
Authors: Joseph Jerrine , Keren Deborah S , Raghavi R , Mary Shamya A , Aruni Wilson 
Published: August 2023

Background:
Green synthesis of nanoparticles has been gaining popularity due to its advantages over chemical synthesis. In the present study, silver nanoparticles (AgNPs) were synthesized by using an aqueous solution of Phyllanthus amarus leaves extract as a reducing agent. The synthesized nanoparticles were characterized using the spectroscopic techniques. The Fourier-transform infrared (FTIR) study confirmed that the seed extract also stabilized the surface of the AgNPs by acting as a capping agent. Moreover, the antibacterial activity of the plant NPs was also assessed. The synthesized nanoparticles as well as P. amarus plant extract were separately tested to examine their antibacterial activities. The activities were tested against various microorganisms, including Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae, and Staphylococcus aureus. The main aim of the present study is to evaluate the green synthesis of nanoparticles using P. amarus seeds and their antibacterial activity assessment.

Method:
Collection and preparation of seed extract, synthesis of AgNPs, characterization of AgNPs using ultraviolet-visible (UV-Vis) absorbance spectroscopy and Fourier transforms infrared spectroscopy, determination of antibacterial activity using pathogens. All in vitro assay data signify the mean ± standard deviation of triplicates was calculated by using the MS word document.

Results:
The reduction of silver nitrate using the plant leaf extract was viewed by the color change in the reaction solutions. The maximum absorbance peak was seen at 400 nm for P. amarus seed extract using UV-Vis spectroscopy and FTIR measurements were carried out for the AgNPs synthesized by the plant extracts. The extracts of P. amarus seeds showed potent antimicrobial activity against Gram-positive and negative bacteria.

Conclusions:
The biosynthesized AgNPs using P. amarus seed extract proved to be excellent agent against pathogens. The present study showed a simple, rapid, and economical route to synthesize AgNPs. The use of P. amarus has the added advantage that this seed can be used by nanotechnology processing industries.
Modified VGG Deep-Learning Architecture for COVID-19 Classification Using Chest Radiography Images
Authors: Anand R. , Sowmya V. , Menon Vijaykrishna , Gopalakrishnan A. , Soman K. P. 
Published: August 2023

Background:
The world faced a deadly disease encounter by the starting of 2020, known as coronavirus disease 2019 (COVID-19). Due to the rapid increase in the counts of COVID cases, the WHO declared the COVID-19 as a pandemic on March 11, 2020. Among the different screening techniques available for COVID-19, radiography of the chest is one of the efficient way for disease detection. While other disease detection techniques take time, radiography takes less time to identify because of the abnormalities caused by the disease in the lungs.

Methods:
In the rapid development era of artificial intelligence and deep-learning techniques, various models are being developed for COVID disease detection. COVID-19 can be easily detected from Chest X-ray images and the pretrained models yield high accuracy with small dataset.

Results:
In this paper, one of the standard deep-learning architectures, VGGNet, is modified for classifying chest X-ray images under four categories. The proposed model uses open source dataset that contains 231, 2503, 1345, and 1341 images of four classes such as COVID, bacterial, normal, and viral chest radiography images, respectively.

Conclusion:
The performance matrices of the proposed work were compared with the five benchmark deep-learning architectures namely VGGNet, AlexNet, GoogLeNET, Inception-v4, and DenseNet-201.
Histopathological Changes in Placenta in Pregnancy-Induced Hypertensive Patients and Correlation with Fetal Outcome – A Tertiary Care Hospital Study
Authors: Samaddar Aparajita , Roy Utso Guha , Saha Ipsita , Mangal Srishtidhar 
Published: August 2023

Background:
The placenta, fetus, and mother form a composite functional equilibrium during prenatal period. The dysfunction of placenta often leads to preeclampsia/eclampsia. They remain the major causes of maternal and perinatal mortality. It has been revealed that there is a clear relationship between placental pathology and intrauterine growth restriction in preeclampsia. Aim of this study was to evaluate the histomorphological changes of placenta in pregnancy induced hypertensive disorders and to compare those with normal placenta and to correlate the findings with foetal outcome in terms of APGAR score and birth weights of newborn babies.

Materials and Methods:
This was an analytical cross-sectional study. We included 50 pregnant women with pregnancy induced hypertension as cases and 50 normotensive pregnant women as controls. Specimens of placenta from each cases and controls were collected after delivery and processed by routine histopathology technique. We looked for morphological and histological changes in placenta. We also collected the data on APGAR scores, birth weight and fetal death, if any. Microsoft Excel 2013 and Minitab version 17 were utilized for statistical analysis.

Results:
We found statistically significant reduction in placental weight, diameter and thickness among cases compared to controls and there is also a strong correlation with the histopathological changes of placenta in hypertensive mothers to fetal weight. Birth weights of newborns to hypertensive mothers were significantly lower than those of normotensive mothers.

Conclusion:
Hypertensive disorders of Pregnancy causes significant histomorphological changes in placenta leading to adverse foetal outcome.
Spectrum of Bacterial Conjunctivitis in Southern Pakistan
Authors: Fatima Tazeen , Malik Faisal Riaz , Ahmed Ayesha , Khan Erum , Shakoor Sadia 
Published: August 2023

Background:
Bacterial conjunctivitis contributes largely to ocular morbidity in both developed and developing countries. The spectrum of conjunctivitis is influenced by geographic and climatic factors. One previous report from Pakistan had shown adenoviruses to be predominantly responsible for keratoconjunctivitis, however little is known about the bacterial agents responsible for conjunctivitis. In this study, we report the microbial etiology of bacterial conjunctivitis by performing a 10-year retrospective review of laboratory data.

Methods:
All conjunctival cultures received at the Aga Khan University Clinical Microbiology Laboratory from 2004 to 2013 with bacterial growth were identified through a laboratory database. Information on patients' demographic characteristics, identification of organisms, and antibiotic susceptibility was obtained from an archived database of laboratory records. Data were entered and analyzed in MS Excel.

Results:
A total of 1534 conjunctival culture samples were received at the clinical microbiology laboratory during the 10-year study period (2004–2013), of which 375 showed bacterial growth. Staphylococcus aureus was found to be the most common bacterial cause of conjunctivitis. Streptococcus pneumoniae was seen predominantly in children aged 0–14 years. Higher trends of resistance were noticed for trimethoprim-sulfamethoxazole and erythromycin.

Conclusions:
The bacterial profile of ocular surface cultures from patients with conjunctivitis showed prevalence of S. aureus in all ages and S. pneumoniae in children, with pseudomonal infections common in older age groups, likely associated with the use of contact lenses. Antibiotic susceptibility testing showed high level of resistance to topical antibiotics. Studies designed to address the challenges of antibiotic susceptibility testing against topical antibiotics are needed to inform empiric treatment guidelines in various age groups.
Unmasking Previously Unrecognized Peripheral Arterial Disease in Patients with Coronary Artery Disease Using Continuous Wave Doppler Using Continuous Wave Doppler Imaging
Authors: Nair Rajesh , Sud Rahul , Swamy Ajay , Patra Vikram 
Published: August 2023

Background:
It is well established that a significant number (30%) of patients suffering from coronary artery disease (CAD) also have underlying peripheral artery disease (PAD) as the only underlying risk factor. They are at increased risk of myocardial infarction as multiple vascular beds beyond the extremities are likely to be affected by atherosclerosis. A large percentage of high-risk patients with the coexistent disease may not be diagnosed with underlying PAD, as they were largely asymptomatic. This study aims to assess the prevalence of previously unrecognized PAD among patients with CAD, the risk association for various risk factors for CAD and PAD and also to determine the relationship between the presence of PAD and severity of CAD.

Methods:
A 3 years randomized, prospective, cross-sectional study done at Command Hospital Airforce Bangalore, India on patients of CADs, who were planned to undergoing coronary angiogram, were evaluated for the presence of PAD in lower extremities. The interactions between risk factors, Ankle Brachial Index (ABI), and CAD were examined by multiple stepwise regression analysis.

Results:
A total of 300 patients were studied. PAD has a prevalence of 14% in patients with CAD and it was strongly associated with disease severity. There was statistically significant association in patients with CAD which coexistent PAD with age (P = 0.001), smoking (P = 0.014), hypertension (P = 0.015), diabetes (P = 0.043), obesity (P= 0.004), history of previous cerebrovascular accident (P = 0.0138), number of coronaries involved (P = 0.02) and low-density lipoprotein (P = 0.001) levels as compared to CAD not associated with PAD.

Conclusions:
The high prevalence of PAD in patients who have CAD confirms the importance of active screening for PAD using ABI and requires aggressive management. We emphasize that clinicians should keep in mind that CAD patients would have other co-morbid vascular manifestations like PAD in order to minimize morbidity and mortality in these patients.
Serum Levels of Interleukin-6, Ferritin, C-Reactive Protein, Lactate Dehydrogenase, D-Dimer, and Count of Lymphocytes and Neutrophils in COVID-19 Patients
Authors: Kadhim Ali Saad , Abdullah Younus Jasim 
Published: August 2023

Background:
Since its first emergence in Wuhan city, China, severe acute respiratory syndrome coronavirus 2, which is responsible for the pandemic COVID-19, has become a significant health problem all over the world affecting over 2.1 million people globally.

Methods:
The current study aimed to investigate serum levels of interleukin 6 (IL-6), ferritin, C-reactive protein (CRP), lactate dehydrogenase (LDH), D-dimer as well as lymphocytes and neutrophils count in COVID-19 patients, and to clarify the correlation of these parameters with disease severity and progression. For these purposes, (100) patients with COVID-19 (confirmed by polymerase chain reaction) and (20) apparently healthy people (with matched age and sex) were included in the current study and considered as a control group.

Results:
All study population (patients and control) were subjected to the evaluation of serum levels of IL-6, ferritin, CRP, LDH, D-dimer, as well as lymphocytes and neutrophils, count. COVID-19 patients showed a significant elevation in the levels of all parameters included in this study when compared with healthy controls. We also found that all of IL-6, ferritin, CRP, LDH, D-dimer are significantly associated with the severity of the COVID-19 symptoms.

Conclusion:
Lymphopenia and increased neutrophils were also effectively correlated with disease progression. In line with these results, we concluded a proportional correlation between the aforementioned parameters and COVID-19 suggesting the uses of these tests to the diagnosis of critical cases.
Variability in Linear Polypeptide Stabilizes Proteoglycan Than Zinc Finger Protein in Vascular Smooth Muscle Cells
Authors: Rajamani Karthikeyan D 
Published: August 2023

Background:
Structural and physicochemical topologies of proteins play a considerable role in differentiating the functional properties of the biological system. We aimed to study the physicochemical similarities, structural and functional differences of versican (VCAN) and early growth response (Egr) proteins involved in vascular injuries. Methods: For the primary structure prediction, the proteomic tools Expasy's Protparam is used, likewise, for secondary structure and content prediction SOPM and SOPMA tool is used. The transmembrane regions in VCAN and EGR proteins are predicted through SOSUI (Classification and Secondary Structure Prediction of Membrane Proteins) server. The CYSREC tool is used to identify the presence of disulphide bonds in all the VCAN and EGR proteins, additionally through homology modelling the disulphide bonds are visualized and structure of the modelled proteins are validated through Rampage (Ramachandran plot), ProQ (Protein Quality Server) and ProSA (Protein Structure Analysis) server.

Results:
VCAN and Egr proteins resemble hydrophilic in nature, similarly negative score of the grand average of hydropathicity index confirms hydrophilic nature. The maximum molecular weight for VCAN is observed as 39265 and 61623 Dalton for EGR protein. VCAN proteins showed a higher level of basic residues except Q86W61, while all the Egr proteins were acidic residues. The extinction coefficient (EC) has unique absorbance at 280 nm wavelength. Based on the aliphatic index (AI ≥ 45) and instability index (II ≥ 40) most of the VCAN and Egr proteins were unstable. The Classification and Secondary Structure Prediction of Membrane Proteins server classifies all Egr and few VCAN and proteins are soluble nature. Secondary structure content prediction and SOPM server show most of the VCAN proteins are beta sheets and many Egr proteins are alpha-helical, while few with mixed structures. Besides these differences, the VCAN protein stability was identified by most probable disulfide (SS) bridges using CYS_REC tool and confirmed by homology modeling in tertiary structure. Whereas the probable disulfide bonds in Egr proteins were not identified.

Conclusion:
The findings with these functional and structural properties will add an extra room in understanding their dual role.
Detection of Human Papilloma Virus in Prostate Tissue from Prostatic Cancer Patients in AL Najaf, Iraq
Authors: Al Ramahy Anwar Abd Hashim 
Published: August 2023

Background:
The prostatic tumor was the third form of cancer in males being >55 years of age, after the pulmonary and colon cancer. The prostate was infected by many viruses causing repeated or chronic inflammation or enhance carcinogenesis. The aim of this study was to determine the presence and prevalence of human papillomavirus (HPV) in the prostate tissue of patients with prostate cancer.

Methods:
Prostate tissue samples were collected from paraffin embedding block of 70 patients diagnosed with HPV from archives of AL-Sadder Hospital in AL-Najaf City and some archives of private histopathology laboratories from January 2016 to December of the same year. We investigated the presence of HPV DNA using the polymers chain reaction technique polymerase chain reaction (PCR).

Results:
The sample size was 60 patients and 10 as control. The number of benign patients is 40/60 (66.7%), whereas the number of malignancies patients is 20/60 (33.3%). There are 13/60 prostatic patients who have HPV. Eight patients of them have a malignancy prostatic tumor and five with a benign prostatic tumor. There is a highly significant association between HPV and malignancy compared with the benign prostatic disease (P = 0.016). PCR results were showed a highly significant specificity (P < 0.001) to detect the presence of HPV in malignancy and benign prostatic disease. PCR results showed a highly significant specificity (P < 0.001) to detect the presence of HPV in malignancy and benign prostatic disease.

Conclusions:
Our study showed an association between prostatic disease and the presence of HPV DNA in samples, but there is a highly significant difference in HPV prevalence between the malignant prostate cancer and benign prostate tissue samples.
Antimicrobial Traits of Different Medicinal Plants Locally Available in Bangladesh
Authors: Hossaini Farzana , Das Nayan Chandra , Hossaini Fahamida , Acharjee Mrityunjoy , Munshi Saurab Kishore 
Published: August 2023

Background:
Medicinal plants have been commonly used in traditional medicine against different ailment from ancient time. The present study was an attempt to determine the anti-bacterial traits of commonly available medicinal plants, especially having topical uses.

Methods:
A total of four types of locally available medicinal plant samples inclusive of Turmeric (Curcuma longa, rhizome), Marigold (Tagetes minuta, leaves), Durva Grass (Cynodon dactylon, whole plant), and Hibiscus (Hibiscus rosa-sinensis, leaves) were collected. Crude, hot water, and solvent (ethanolic and methanolic extracts of the samples) were tested for their antimicrobial traits by agar well-diffusion method on Mueller-Hinton agar. The minimal inhibitory concentration (MIC) of each sample was determined by the microdilution technique. Microbiological quality of the samples was also checked.

Results:
Microbial contaminations were found in all the samples. Beside viable bacteria and fungi, all samples contained Staphylococcus spp. and Pseudomonas spp. The proliferation of Klebsiella spp. was observed in the majority of the samples. Notable antimicrobial activities were exhibited by the medicinal plant parts tested in this study. Ethanolic and methanolic extracts showed remarkable efficiency in eliminating the growth of tested bacterial isolates although crude and hot water extracts projected no considerable inhibitory effects. The majority of the samples had a MIC value at 12 mg/mL.

Conclusion:
The findings of the present study reveal that the medicinal plant samples had remarkable antimicrobial traits which portray their potential for the commercial application against different infectious diseases.
Nitrate-Nitrite Toxicosis Associated with Duckweed (Portulaca Oleracea L.) (Portulacaceae) Consumption in a Herd of Sheep in Kenya
Authors: Nguta Joseph Mwanzia 
Published: August 2023

Background:
Nitrate-nitrite toxicosis associated with the consumption of duckweed (Portulaca oleracea L.) (Portulacaceae) was diagnosed in a small herd comprising of 11 sheep in Korayo Sub Location, Rangwe Sub County of Homabay County in Kenya.

Methods:
The clinical signs were dyspnea, froth from the mouth, labored breathing, incoordination, tachycardia, increased urination, neck, fore and hind limb distension, aggressive movements, bloat, convulsions, and coma, before death.

Results:
All the 11 sheep died of intoxication before institution of therapy. Brownish mucous membranes and chocolate colored and poorly clotted blood were the significant necropsy observations. Mild pulmonary edema was characterized by the presence of limited froth in the trachea and bronchioles. At postmortem, the liver of the sheep was grossly congested, and the cardiac pericardium was inflamed. There was gastritis, enlarged, and congested kidneys with pin-point hemorrhages. Diphenylamine tests carried out on the fed duckweed and on ruminal duckweed contents were positive for nitrates. Duckweed (Portulaca oleracea) fed on sheep had 7.4% nitrate on dry matter basis (DM), whereas ruminal contents had 5.38% nitrate on DM basis. Chocolate-colored blood collected during postmortem had a methemoglobin fraction of 86%. Nitrate levels in pastures of 0.5% and above have been shown to be potentially dangerous, with acute poisoning likely to occur at times when the levels are above 1%. Death in sheep can occur when blood methemoglobin levels are in the range of 67%–90%.

Conclusion:
The observations from the present study are indicative of death in a herd of sheep due to nitrate-nitrite poisoning associated with the consumption of duckweed (Portulaca oleracea).
Prevalence and Antibiotic Susceptibility Pattern of Uropathogenic Escherichia Coli Strains in Sonipat Region of Haryana in India
Authors: Malik Shikha , Rana Jogender Singh , Nehra Kiran 
Published: August 2023

Background:
To study the prevalence and antibiotic susceptibility pattern of uropathogenic Escherichia coli strains in Sonipat region of Haryana in India. Urinary tract infections (UTIs) are among the most widespread infections, caused most frequently by Escherichia coli. The past few years have witnessed a rapid rise in the spread of high antibiotic-resistant forms of these uropathogenic E. coli (UPECs).

Methods:
In the current study, a total of 53 UPEC isolates from patients of all age groups were collected from Sonipat and its adjoining areas from September 2017 to April 2018. All the uropathogens were subjected to antibiotic susceptibility profiling using a set of 23 antibiotics and confirmed using standard microbiological procedures. The isolates which exhibited high degree of multidrug resistance (MDR) were characterized at molecular level using 16S rRNA sequencing.

Results:
The evaluation of age- and gender-wise prevalence of UTI revealed that women (77.3%) were more susceptible to infection as compared to men (22.6%). It was observed that 83% of the collected UPEC isolates exhibited MDR pattern. The isolates exhibited maximum resistance to cephalosporins and fluoroquinolones; and the highest susceptibility (100%) was shown toward tigecycline, followed by 96.2% to colistin, 90.5% to amikacin, 86.7% to fosfomycin, and 84.9% to nitrofurantoin.

Conclusion:
The study concludes that the prevalence of MDR pathogens is high in studied region. For empirical antibiotic therapy, amikacin, fosfomycin, and nitrofurantoin should be preferred; and for MDR uropathogens, colistin, and tigecycline should be recommended.