bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal-Child Microbiome and Impact on Growth and Neurodevelopment in Infants and Children: A Scoping Review
Authors: Mathews Therese , Hayer Shivdeep S. , Dinkel Danae , Hanish Alyson , Poppert Cordts Katrina M. , Rasmussen Heather , Moore Tiffany 
Published: January 2023

Background Pathologic changes in the microbiome (dysbiosis) have been implicated in affecting the growth and neurodevelopment of infants and children. There is evidence to suggest that prenatal and postnatal stressors may be a factor in dysbiosis and there is also a growing body of evidence to suggest that interventions may reduce this negative impact. A scoping review was undertaken to identify association between maternal and/or child microbiome with child growth and neurodevelopment. Additionally, intervention studies such as use of nutritional supplementation and its impact on the microbiome, growth and neurodevelopment were reviewed. Methods An exhaustive literature search identified 654 relevant citations. After review of abstracts, 557 were eliminated, and 97 remained for full text review. We identified and reported on 42 articles which met inclusion criteria. Results Seven studies examined associations between microbiome and neurodevelopment and 36 studies evaluated anthropometric measurements, most commonly weight, and microbiota relationships. One study evaluated both growth and neurodevelopment and microbiota. Fourteen studies evaluated supplemental nutrients. Preterm, low birth weight (LBW), and very low birth weight (VLBW) infants were most studied. Findings were inconclusive for consistent associations between microbiota and growth and neurodevelopment. Further, there were no consistent conclusive changes with prescribed treatment interventions. Discussion There is a need for high-quality longitudinal studies evaluating repeated developmental assessment measures using consistent microbial analysis techniques to inform conclusions regarding the association between microbiome and infant and child growth and neurodevelopment. Additional intervention studies that may mitigate dysbiosis are warranted.
Shotgun metagenomic sequencing in culture negative microbial keratitis
Authors: Borroni Davide , Bonzano Chiara , Sánchez-González José-María , Rachwani-Anil Rahul , Zamorano-Martín Francisco , Pereza-Nieves Jorge , Traverso Carlo Enrico , García Lorente María , Rodríguez-Calvo-de-Mora Marina , Esposito Alfonso , Godin Fernando , Rocha-de-Lossada Carlos 
Published: January 2023

Purpose To evaluate the microbiota of culture negative Corneal Impression Membrane (CIM) microbial keratitis samples with the use of shotgun metagenomics analysis. Methods DNA of microbial keratitis samples were collected with CIM and extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre). DNA was fragmented by sonication into fragments of 300 to 400 base pairs (bp) using Bioruptor® (Diagenode, Belgium) and then used as a template for library preparation. DNA libraries were sequenced on Illumina® HiSeq2500. The resulting reads were quality controlled, trimmed and mapped against the human reference genome. The unmapped reads were taxonomically classified using the Kraken software. Results 18 microbial keratitis samples were included in the study. Brevundimonas diminuta was found in 5 samples while 6 samples showed the presence of viral infections. Cutibacterium acnes, Staphylococcus aureus, Moraxella lacunata and Pseudomonas alcaligenes were also identified as the presumed putative cause of the infection in 7 samples. Conclusions Shotgun sequencing can be used as a diagnostic tool in microbial keratitis samples. This diagnostic method expands the available tests to diagnose eye infections and could be clinically significant in culture negative samples.
Double scleral tunnel technique to prevent glaucoma drainage device exposure
Authors: Fahy Eamonn T , Garg Anurag , Lim Kin Sheng 
Published: January 2023

Purpose To describe a new surgical technique designed to reduce the risk of tube exposure following glaucoma drainage device (GDD) surgery. Methods A retrospective, single-center study included all patients who underwent GDD surgery by a single surgeon between January 2018 and December 2019, using a double scleral tunnel technique to secure the tube to the sclera. A 23-gauge needle was used to create a 3 mm partial thickness scleral tunnel near to the GDD plate, with a second partial thickness scleral tunnel created 2.5 mm from the surgical limbus into the anterior chamber (AC). The silicone tube was passed through both scleral tunnels and into the AC. Results 46 GDD surgeries were performed during the time frame. The mean age of patients was 65 ± 13 years. Mean follow up duration was 27.7 ± 8.15 months (range 11 to 44). Significant reductions in intraocular pressure and number of glaucoma medications were achieved. Zero exposures were recorded occurring during follow-up. Conclusion This double scleral tunnel technique to secure the GDD silicone tubing firmly to the sclera may reduce micro-movements which have been purported to be associated with tissue erosion post GDD surgery. The addition of this simple technique to existing GDD surgery may reduce the risk of post-operative tube exposure.
Factors affecting indoor environmental air quality of non-smoking residences in Perth, Western Australia
Authors: Gilbey Suzanne E , Reid Christopher M , Zhao Yun , Soares Mario J , Rumchev Krassi B 
Published: January 2023

This study investigated associations between a range of indoor air pollutants with household characteristics in 111 non-smoking residences located in Perth, Western Australia. Overall, the mean 24-hour indoor concentrations for PM10 and PM2.5 were below and above the World Health Organisation (WHO) (ambient) guideline values (GV) of 45 µg/m3 and 15 µg/m3, respectively. Concentrations of measured gases were low, other than nitrogen dioxide (NO2) which recorded a mean 24-hour concentration above the WHO GV (0.013ppm). Higher concentrations of total volatile organic compounds (TVOC) and carbon dioxide (CO2) were observed as the number of household occupants increased. The type of heating and/or cooling appliances used was associated with higher concentrations of TVOC, CO2, carbon monoxide (CO), NO2 and all size fractions of particulate matter (PM). Households that were opened daily for fresh air recorded higher concentrations of all PM size fractions and NO2 when compared to those that were infrequently aired. These findings contribute to a better understanding of household characteristics that influence indoor air quality (IAQ) in homes where cigarette smoke, a significant contributor to residential air pollution, has been eliminated. These results are useful for modifying some behavioural choices by home occupants and can also inform meaningful risk mitigation strategies aimed at improving IAQ in residential settings.
Endovascular Aortic Repair for a Symptomatic Mycotic Aneurysm With Listeria monocytogenes: A Case Report
Authors: Masuda Noriyasu , Azuma Takashi , Furukawa Hiroshi , Uwabe Kazuhiko 
Published: January 2023

Purpose To report a case of successful endovascular aortic repair for a symptomatic mycotic abdominal aortic aneurysm infected with Listeria monocytogenes. Case Report We report the case of an 88-year-old woman who presented with acute abdominal pain and vomiting. Approximately a year prior to her presentation, the patient was diagnosed with a mycotic abdominal aortic aneurysm with Listeria monocytogenes and was treated conservatively for more than 2 months at another hospital. At our hospital, contrast-enhanced computed tomography revealed an abdominal aortic aneurysm and an aneurysm of the left internal iliac artery. Endovascular aortic repair was performed successfully. At 16 months after the surgery, the patient remained asymptomatic on long-term antibiotics, and there was no enlargement of the aneurysm. Conclusion Endovascular aortic repair and lifelong antibiotics may be an alternative therapy for mycotic abdominal aortic aneurysms. However, the risk of recurrent infection is high and warrants long-term follow-up.
Scapholunate ligament reconstruction using a part of the extensor carpi radialis brevis tendon through a dorsal approach
Authors: Oeckenpöhler Simon , Wieskötter Britta , Aitzetmüller Matthias , Klietz Marie Luise , Royeck Thorben , Langer Martin Franz 
Published: January 2023

Thirty-six patients were assessed after scapholunate ligament reconstruction using a portion of the extensor carpi radialis brevis through a dorsal approach. The median age was 53 years. Most (27/38) were graded as scapholunate advanced collapse Grade I. At a median of 47 months after treatment, hand function using the Disabilities of Arm, Shoulder and Hand Questionnaire was 12. The postoperative range of wrist flexion and extension movement was 77% and grip strength 92% compared with the uninjured side. The median patient satisfaction was rated as 9/10. Median pain scores without and with load, using the numeric pain scale (0–10), were 1 and 3, respectively. This reconstruction leads to initial normalization of radiological features, such as scapholunate interval, scapholunate and radiolunate angles, but a notable loss of the immediate postoperative reduction was observed in long-term follow-up, which was not accompanied by any deterioration in the clinical examination. This technique, even in scapholunate advanced collapse type I wrists, resulted in long-term, improved outcomes compared with other techniques. Level of evidence: IV
An analytics approach to health and healthcare in citizen science communications on Twitter
Authors: Martínez-Martínez Fernando , Roldán-Álvarez David , Martín Estefanía , Hoppe H Ulrich 
Published: January 2023

Background Citizen science is a growing practice in which volunteers, including non-scientists, conduct or contribute to research by collecting and analyzing data. The increasing importance of citizen science in the last years has led to an increased interest in detecting how citizen science can contribute to scientific advancements in different areas. Recent research shows that citizen science has become a means of engagement between scientist and the public, encouraging scientific curiosity and promoting scientific knowledge. Methods In this article, we report on how to apply computational analysis techniques to Twitter messages to reveal the impact of citizen science in health-related areas. The main objectives are (1) to characterize central topics of these discussions, and (2) to identify particularly important actors in these social media networks. Results For the topics, our findings suggest that sustainable development goals, technologies and health, and COVID-19 are those most addressed by the users. Other topics represented in the data are cancer, public health, mental health, and health and well being of sea and earth living creatures related to sustainable development goals. Conclusion Based on our results, those entities or actors who are most cited and retweeted are Twitter accounts of projects and not primarily individual professionals or citizen scientists.
Chronic Illness Patients’ Perceptions of Quality of Care During Hospitalization: A Qualitative Study
Authors: Tunsi Afnan , Alhelal Raneem , Mujalled Raneem , Alhadrami Erghad , Alsulami Rahaf , Alhofaian Aisha , Alaamri Marym , Babkair Lisa 
Published: January 2023

Introduction The rising prevalence of patients living with chronic illnesses and frequent hospitalizations represent many challenges in delivering high-quality care. Assessing patients’ perceptions and needs regarding the quality of health care services is a critical step in improving quality of care. Objective This study sought to explore the perception of quality of care from the perspectives and experiences of chronically ill patients during hospitalization. Methods This study was conducted using a qualitative descriptive design. Face-to-face semi-structured individual interviews were conducted with 15 chronically ill patients at their bedside during their current hospital admission. Analysis followed Braun and Clarke's thematic analysis approach. Results Overall patients were pleased with their hospitalization experience; however, they highlighted many aspects of care that can be addressed to improve the quality of care provided. Five themes were identified from the analysis as follows: defining quality of care; aspects related to the healthcare providers; unmet care needs; patient's involvement in healthcare decisions and care expectations and outcomes. Conclusion A key finding of this study was the association between quality of care and healthcare providers’ skills, clinical competence, and attitudes. Poor communication and discoordination of care were the most significant impediment expressed. Actively involving patients in care may be effective when combined with meeting expectations. The findings of this study helped to identify the strength points and areas of improvement that are the cornerstone for developing tailored and patient-centered strategies to improve the quality of care during hospitalizations among chronically ill Saudis.
Knowledge, Attitudes, and Practices of Hypertensive Patients Towards Stroke Prevention Among Rural Population in Saudi Arabia: A Cross-Sectional Study
Authors: Alhowaymel Fahad M. , Abdelmalik Mohammed A. , Mohammed Almoez M. , Mohamaed Mohamaed O. , Alenezi Atallah 
Published: January 2023

Introduction Stroke is a significant health problem and is considered one of the leading causes of mortality and permanent disability worldwide. Hypertension is a primary risk factor for stroke. Thus, hypertensive patients’ knowledge, attitudes, and practices (KAP) are essential in preventing stroke. Objective To examine hypertensive patients’ KAP towards stroke prevention among rural population in Saudi Arabia. Methods The study utilized a cross-sectional design. The data were collected using a convenience sampling technique from hypertensive patients in the northwestern rural areas of Riyadh Province. A self-administered questionnaire was adapted from the Stroke Recognition Questionnaire (SRQ) and utilized to assess KAP towards stroke prevention among rural hypertensive patients. The STrengthening of the Reporting of OBservational studies in Epidemiology (STROBE) checklist was used to report the study. Results A total of 196 hypertensive patients participated in the study. The total mean scores categorization into three quartiles showed that most respondents had poor levels of KAP towards stroke prevention. Weak positive correlations were found between KAP of patients. There were statistically significant differences between respondents’ attitudes and practices with their age, education, marital status, and occupational status. Discussion This study denoted poor levels of KAP towards stroke prevention among hypertensive patients living in rural areas. This study showed the importance of further considering rural population by which improving their health and quality of life. Extending health educational programs and behavior-changing strategies to rural areas is essential to increase hypertensive patients and general public awareness about their stroke prevention and other health-related concerns.
Bedside lung ultrasound for the diagnosis of pneumonia in children presenting to an emergency department in a resource-limited setting
Authors: Amatya Yogendra , Russell Frances M. , Rijal Suraj , Adhikari Sunil , Nti Benjamin , House Darlene R. 
Published: January 2023

Abstract
Background
Lung ultrasound (LUS) is an effective tool for diagnosing pneumonia; however, this has not been well studied in resource-limited settings where pneumonia is the leading cause of death in children under 5 years of age.

Objective
The objective of this study was to evaluate the diagnostic accuracy of bedside LUS for diagnosis of pneumonia in children presenting to an emergency department (ED) in a resource-limited setting.

Methods
This was a prospective cross-sectional study of children presenting to an ED with respiratory complaints conducted in Nepal. We included all children under 5 years of age with cough, fever, or difficulty breathing who received a chest radiograph. A bedside LUS was performed and interpreted by the treating clinician on all children prior to chest radiograph. The criterion standard was radiographic pneumonia, diagnosed by a panel of radiologists using the Chest Radiography in Epidemiological Studies methodology. The primary outcome was sensitivity and specificity of LUS for the diagnosis of pneumonia. All LUS images were later reviewed and interpreted by a blinded expert sonographer.

Results
Three hundred and sixty-six children were enrolled in the study. The median age was 16.5 months (IQR 22) and 57.3% were male. Eighty-four patients (23%) were diagnosed with pneumonia by chest X-ray. Sensitivity, specificity, positive and negative likelihood ratios for clinician’s LUS interpretation was 89.3% (95% CI 81–95), 86.1% (95%CI 82–90), 6.4, and 0.12 respectively. LUS demonstrated good diagnostic accuracy for pneumonia with an area under the curve of 0.88 (95% CI 0.83–0.92). Interrater agreement between clinician and expert ultrasound interpretation was excellent (k = 0.85).

Conclusion
Bedside LUS when used by ED clinicians had good accuracy for diagnosis of pneumonia in children in a resource-limited setting.
Pulmonary artery embolism: comprehensive transcriptomic analysis in understanding the pathogenic mechanisms of the disease
Authors: Gromadziński Leszek , Paukszto Łukasz , Lepiarczyk Ewa , Skowrońska Agnieszka , Lipka Aleksandra , Makowczenko Karol G. , Łopieńska-Biernat Elżbieta , Jastrzębski Jan P. , Holak Piotr , Smoliński Michał , Majewska Marta 
Published: January 2023

Abstract
Background
Pulmonary embolism (PE) is a severe disease that usually originates from deep vein thrombosis (DVT) of the lower extremities. This study set out to investigate the changes in the transcriptome of the pulmonary artery (PA) in the course of the PE in the porcine model.

Methods
The study was performed on 11 male pigs: a thrombus was formed in each right femoral vein in six animals, and then was released to induce PE, the remaining five animals served as a control group. In the experimental animals total RNA was isolated from the PA where the blood clot lodged, and in the control group, from the corresponding PA segments. High-throughput RNA sequencing was used to analyse the global changes in the transcriptome of PA with induced PE (PA-E).

Results
Applied multistep bioinformatics revealed 473 differentially expressed genes (DEGs): 198 upregulated and 275 downregulated. Functional Gene Ontology annotated 347 DEGs into 27 biological processes, 324 to the 11 cellular components and 346 to the 2 molecular functions categories. In the signaling pathway analysis, KEGG ‘protein processing in endoplasmic reticulum’ was identified for the mRNAs modulated during PE. The same KEGG pathway was also exposed by 8 differentially alternative splicing genes. Within single nucleotide variants, the 61 allele-specific expression variants were localised in the vicinity of the genes that belong to the cellular components of the ‘endoplasmic reticulum’. The discovered allele-specific genes were also classified as signatures of the cardiovascular system.

Conclusions
The findings of this research provide the first thorough investigation of the changes in the gene expression profile of PA affected by an embolus. Evidence from this study suggests that the disturbed homeostasis in the biosynthesis of proteins in the endoplasmic reticulum plays a major role in the pathogenesis of PE.
Morphological variability of wild-growing crown imperial (Fritillaria imperialis L.) germplasm in central region of Iran—implications for in-situ conservation initiatives
Authors: Moradi Mohammad , Khaleghi Alireza , Khadivi Ali 
Published: January 2023

Abstract
Background
Crown imperial (Fritillaria imperialis L.) is a threatened bulbous plant which has great ornamental and medicinal values and importance. In the present study, a total of 100 specimens of wild-growing F. imperialis from 10 natural areas of Markazi province, Iran, representing one of the main centers of genetic diversity of this species, were evaluated using 37 phenotypic attributes during April 2021.

Results
High level of genetic variation within populations (75%) and low levels of genetic variation among populations (25%) was revealed. The highest coefficient of variation (CV) was found in leaf trichome (82.00%) and then margin of crown leaves (80.44%). In addition, flower color (CV = 50.86%), flower number (CV = 44.61%), peduncle diameter (CV = 33.44%), and plant length (CV = 32.55%)—all important from an ornamental point of view- showed relatively high CV values. The CV was the lowest for flower shape, filament color, bulb shape, bulblet number, and floral scent. Ward cluster analysis identified two main clusters, containing 14 and 86 specimens, respectively. The first group consisted mainly of specimens from the adjacent Shahbaz and Rasvand populations. According to the principal component analysis (PCA), the first six components of data accounted for 88.36% of total variance. The Shahbaz-1, Shahbaz-2, Shahbaz-6, Shahbaz-7, Shahbaz-9, and Bolagh-8 specimens showed the highest variation and were separated from others, which they can be used further in breeding programs, while Sarchal-2, Bolagh-3, and Chepeqli-4 specimens showed the lowest variability. Moreover, the studied populations were clustered into four distinct groups, each including populations that were geographically close to one another.

Conclusions
Although the examined specimens revealed high genetic diversity herein, the results indicated that wild-growing populations of F. imperialis are still at risk suffering from overcollection in the most of studied areas, especially in Deh-Sad and Tureh.
Goal-Directed Fluid Therapy and major postoperative complications in elective craniotomy. A retrospective analysis of a before-after multicentric study
Authors: Le Guen Morgan , Le Gall-Salaun Amandine , Josserand Julien , Gaudin de Vilaine Augustin , Viquesnel Simon , Muller Damien , Rozec Bertrand , Billet Kévin Buffenoir , Cinotti Raphaël ,   the Société Française d’Anesthésie-Réanimation–SFAR Research Network, Yavchitz Amélie , Sigault Stéphanie , Mazereaud Aurélien , Bezu Lucilia , Léger Maxime , Evain Jean-Noël 
Published: January 2023

Abstract
Background
Goal-Directed Fluid Therapy (GDFT) is recommended to decrease major postoperative complications. However, data are lacking in intra-cranial neurosurgery.

Methods
We evaluated the efficacy of a GDFT protocol in a before/after multi-centre study in patients undergoing elective intra-cranial surgery for brain tumour. Data were collected during 6 months in each period (before/after). GDFT was performed in high-risk patients: ASA score III/IV and/or preoperative Glasgow Coma Score (GCS) < 15 and/or history of brain tumour surgery and/or tumour greater size ≥ 35 mm and/or mid-line shift ≥ 3 mm and/or significant haemorrhagic risk. Major postoperative complication was a composite endpoint: re-intubation after surgery, a new onset of GCS < 15 after surgery, focal motor deficit, agitation, seizures, intra-cranial haemorrhage, stroke, intra-cranial hypertension, hospital-acquired related pneumonia, surgical site infection, cardiac arrythmia, invasive mechanical ventilation ≥ 48 h and in-hospital mortality.

Results
From July 2018 to January 2021, 344 patients were included in 3 centers: 171 in the before and 173 in the after (GDFT) period. Thirty-six (21.1%) patients displayed a major postoperative complication in the Before period, and 50 (28.9%) in the After period (p = 0.1). In the propensity score analysis, we matched 48 patients in each period: 9 (18.8%) patients in the After period and 14 (29.2%) patients in the Before period displayed a major perioperative complication (p = 0.2). Sixty-two (35.8%) patients received GDFT in the After period, with great heterogeneity among centers (p < 0.05).

Conclusions
In our before-after study, GDFT was not associated with a decrease in postoperative major complications in elective intra-cranial neurosurgery.