bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antidepressant Effects of Hydroalcoholic Extract of Origanum Vulgare L. Using Adult Male Mice Model of Depression Induced by Reserpine
Authors: Parandin Rahmatollah , Abbasi Faeze 
Published: April 2023

Introduction: Origanum Vulgare (Oregano) is a plant rich in antioxidant compounds and has therapeutic effects. The aim of this study was to investigate the effects of Oregano alcoholic extract on complications of depression in reserpine-poisoned mice.
Methods: In this experimental study, 42 male BALB/c mice were divided into 6 groups of 7, including control (normal saline), negative control (reserpine), fluoxetine (positive control), and three reserpine groups treated respectively with 100, 200 and 400 mg/kg of Oregano via the intraperitoneal route. Depression was evaluated using forced swim test. Brain and serum total antioxidant capacity and malondialdehyde level were also determined. The data were analyzed by SPSS statistical software and One-Way ANOVA test. The significant was shown with (p≤0.05).
Results: Negative control group significantly increased immobility time and decreased swimming time compared to the control group in the forced swim test (p≤0.001) and the extract at levels of 200 and 400 mg/kg similar to fluoxetine significantly reduced the immobility and increased the swimming time (p≤0.001). Reserpine in the negative control group decreased the antioxidant capacity and increased the level of malondialdehyde in serum and brain compared to the control group (p≥0.001).Compared to the negative control group, the extract at the level of 200 mg/kg, similar to fluoxetine, increased the antioxidant capacity in the brain (p≥0.01) and decreased the level of malondialdehyde in the serum and brain (p≥0.001). In addition, the extract at level of 400 mg/kg similar to fluoxetine significantly increased the total antioxidant capacity and decreased the level of malondialdehyde in serum and brain (p≤0.001) compared to control group.
Conclusion: Oregano seems to have significant potential in reducing depressive symptoms and reducing oxidative stress due to its active antioxidant compounds.
A casa como lugar de estudo e vinculação
Authors: Teixeira Ângela Castelo Branco , Siqueira Giuliano Tierno de 
Published: April 2023

Considerando que todas as casas podem ser lugares de estudos e vinculações, propomos um ensaio sobre a experiência d’A Casa Tombada, um espaço que estuda e pesquisa os campos da arte, da cultura e da educação, a partir de seus princípios (valores), vértices (modos de estudar) e vórtices (encontro com o acontecimento). Pensaremos a casa como o lugar do exercício da palavra oral e escrita, da conversa como experiência; da prática da confiança no processo dialético de acolher e ser acolhido nos pensamentos, sentires e dizeres de uns para os outros num território de estudos; e, por fim, da prática de atenção cotidiana e do risco de se fazer perguntas à própria experiência.
O ensino como ato poético
Authors: Moschen Simone Zanon , Castello Branco Lucia , Chraim Rosi Isabel Bergamaschi 
Published: April 2023

O que se pode dizer sobre formação das novas gerações nas condições atuais? O que se pode propor como princípio orientador para o ensino, num país em que a palavra se vê aprisionada no sentido único que estreita os limites dos mundos que ela nomeia e produz? Teria a experiência literária, como experiência singular do sujeito com a palavra, no dizer de Blanchot (2005), algo a nos indicar sobre a produção de frestas em um mundo que parece trabalhar na direção de um fechamento sobre si mesmo? É na tentativa de estender o alcance dessas perguntas e de esboçar modos de encaminhá-las que este dossiê se organiza, conjugando sete artigos: cinco de pesquisadores brasileiros – UFMG, UFRGS, UFBA, UFAC, UNESP, UFJF – e dois de pesquisadores estrangeiros – Instituto de Educação da Universidade de Lisboa, Instituto Politécnico de Lisboa e da Faculdade Latino-Americana de Ciências Sociais (FLACSO).
Diastolic heart failure: boundaries of term application
Authors: Kalyuzhin V. V. , Teplyakov A. T. , Bespalova I. D. , Kalyuzhina E. V. , Chernogoryuk G. E. , Terentyeva N. N. , Grakova E. V. , Kopeva K. V. , Usov V. Yu. , Garganeeva N. P. , Pavlenko O. A. , Gorelova Yu. V. 
Published: April 2023

Important changes regarding the understanding of the pathogenesis of chronic heart failure (CHF) marked the beginning of the millennium, and its first decade was called the decade of diastology. Even though numerous studies convincingly proved that deterioration of the left ventricular (LV) filling pressure often precedes impairment of its systolic function and a number of factors affect (especially at the onset) mainly the diastolic function without changing the conditions of blood ejection, modern classifications and approaches to CHF treatment are primarily based on the results of LV ejection fraction (EF) assessment.In recent years, diastolic heart failure (DHF) has been often overlooked and replaced by the ambiguous term “CHF with preserved EF”. However, sometimes authors use the term DHF extensively, since CHF based on myocardial insufficiency develops only via two mechanisms (systolic and / or diastolic dysfunction), and excluding one of the mechanisms allows to identify the underlying one. The term DHF can be used in clinical practice and cannot be replaced by the diagnosis of CHF with preserved EF. CHF with preserved EF is a broader concept which includes a full spectrum of cardiovascular diseases, complicated by the development of CHF without depression of the global LV contractility and requiring differentiated approaches to therapy. In addition, the results of repeated studies on LVEF in many patients may require reclassification of this CHF phenotype, which is established following the analysis of the baseline value of global LV contractility. We join M.R. Zile in the appeal to stop discriminating against the term “DHF” and present the boundaries of its correct application.
Diagnosis of bacterial infection in patients with COVID-19: is it a simple task? (literature review)
Authors: Karoli N. A. , Rebrov A. P. 
Published: April 2023

Diagnosing bacterial infection in patients with novel coronavirus infection (COVID-19) is not an easy task. Available data suggest that bacterial infection in patients with COVID-19 is rare and occurs in less than 10% of cases. At the same time, data of individual studies and systematic reviews indicate that more than 70% of patients with COVID-19 receive mainly empirical antimicrobial therapy with broad-spectrum antibiotics often before the diagnosis of COVID-19 has been verified. Therefore, this widespread empirical use of antibiotics is not supported by data on the need for their use.The article discusses the literature data on the significance of commonly accepted methods for diagnosing bacterial infection, with an emphasis on laboratory presence / absence tests. In everyday practice, the likelihood of bacterial coinfection in patients with COVID-19 is assessed by clinical presentation of the disease and the results of standard laboratory tests and imaging methods. However, when viral respiratory infection develops, this approach does not always allow to diagnose bacterial coinfection with sufficient significance. This issue may be handled by available modern test systems, the use of a combination of signs or additional laboratory criteria (for example, procalcitonin), and the analysis of the overall clinical presentation by the doctor using knowledge about patient risk groups.
Foresight in the diagnosis of trematodiasis: innovations versus routine methods
Authors: Perina E. A. , Khmelevskaya E. S. , Fedorova O. S. , Ivanov V. V. 
Published: April 2023

Aim. To analyze modern methods for the diagnosis of trematodiasis in experimental and epidemiological studies.Trematodiasis is a group of common parasitic diseases that are a socially sensitive health problem worldwide. According to World Health Organization, more than 250 million people are affected by trematode infections globally. The most common types of human trematode infections are diseases caused by Schistosoma, Fasciola, Clonorchis, and Opisthorchis pathogens. Diagnosis of trematodiasis is often multistage and includes identification of disease symptoms, analysis of medical history, and use of various laboratory tests. Clinical presentation of parasitic infections often varies, making a definitive diagnosis difficult. Various tools are used to diagnose trematode infections: epidemiological criteria, laboratory tests (complete blood count and blood biochemistry, serological methods), instrumental methods (abdominal X-ray and ultrasound), and parasitological techniques, which often have insufficient sensitivity and specificity. Therefore, development of modern and effective non-invasive methods for detection of trematode infections with high sensitivity and specificity, including screening in endemic regions, is relevant.The present review analyzes the results of 90 clinical trials and experimental studies on the diagnosis of trematode infections using the PubMed search engine and the eLibrary database. The review analyzes original articles published from January 1, 2015 to December 31, 2021.Most studies confirm that the absence of a standard diagnostic approach highlights obvious convenience of utilizing a combined approach to reliable diagnosis of trematodiasis. An adequate combination of different diagnostic tests makes it possible to diagnose the disease correctly, devise a correct treatment and follow-up strategy, and organize preventive measures.
Pharmacological effects of a new soluble guanylate cyclase stimulator in experimental pulmonary arterial hypertension
Authors: Bykov V. V. , Bykova A. V. , Stankevich S. A. , Aliev O. I. , Sidekhmenova A. V. , Dunaeva O. I. , Khazanov V. A. , Vengerovskii A. I. , Udut V. V. 
Published: April 2023

Aim. To assess the effect of an indolinone derivative (2-[2-[(5RS)-5-(hydroxymethyl)-3-methyl-1,3-oxazolidine-2yliden]-2-cyanoethylidene]-1H-indole-3(2H)-one (codename – GRS) on right ventricular contractility, endothelial vasodilator function, and histologic changes in the lungs and heart in a rat model of monocrotaline-induced pulmonary hypertension.Materials and methods. Pulmonary arterial hypertension (PAH) was induced in Wistar rats by a single subcutaneous administration of monocrotaline at a dose of 60 mg / kg. Starting from day 15 after PAH induction, the rats received either GRS at a dose of 10 mg / kg or riociguat at a dose of 1 mg / kg orally once a day. Blood pressure in the right ventricle, right ventricular weight, endothelial vasodilator function, and the histologic structure of the lungs and heart were studied after the last administration of test substances.Results. Twenty-eight days after monocrotaline administration, the rats developed PAH, as shown by the increase in the maximal blood pressure in the right ventricle and the right ventricular weight / total heart weight ratio. GRS after multiple administration reduced the maximal blood pressure in the right ventricle, had no significant effect on its contractility, improved endothelial vasodilator function, and normalized blood pressure. Riociguat had a hypotensive effect and did not alleviate endothelial dysfunction in experimental PAH.Conclusion. The indolinone derivative GRS and riociguat, both soluble guanylate cyclase stimulators, lowered blood pressure in the right ventricle. GRS also alleviated endothelial dysfunction in animals with experimental PAH.
Predictive value of inflammatory regulators TGFb1 and CXCL8 in tumor tissue in colorectal cancer
Authors: Bogomolova I. A. , Dolgova D. R. , Antoneeva I. I. , Abakumova T. V. , Myagdieva I. R. , Peskov A. B. , Gening T. P. 
Published: April 2023

Background. Colorectal cancer is ranked third in terms of incidence and second in terms of mortality around the world. Molecular markers of chemoresistance allow to determine the prognosis of the disease and sensitivity of the tumor to drugs.Aim. To assess the predictive value of expression of regulators of tumor-associated inflammation TGFb1 and CXCL8 in the tumor tissue in colorectal cancer.Materials and methods. Patients were divided into 3 groups: group I included patients without relapse of the disease, group II encompassed patients with relapse of the disease (within 6–16 months after the end of chemotherapy), group III included patients with disease progression. Expression of TGFb1 and CXCL8 in the tumor tissue before treatment in patients with stage II–III colorectal cancer (n = 77) was determined using quantitative realtime polymerase chain reaction (PCR) on the Bio-Rad CFX-96 Touch Real-Time PCR Detection System (USA). Statistical data processing was performed using Statistica 13.0 software (StatSoft, USA).Results. We found that in samples of poorly differentiated colorectal cancer, the level of TGFb and CXCL8 mRNA was significantly higher than in moderately and well differentiated tumors. We did not reveal any relationship of the level of TGFb1 and CXCL8 transcripts in tumor samples of patients with stage II–III colorectal cancer with age and the presence of mutations in the EGFR (Epidermal Growth Factor Receptor) signaling pathway (RAS, BRAF). We found a strong positive correlation between the levels of TGFb1 and CXCL8 transcripts for the entire sample of patients with colorectal cancer. We have found that the expression of TGFb1 and CXCL8 genes was significantly higher in the tumor tissue of patients with disease progression.Conclusion. Overexpression of TGFb1 and CXCL8, which are involved in the mechanism of tumor-associated inflammation, can be considered as a negative prognostic factor for the progression-free interval when using the FOLFOX / XELOX regimen for the treatment of colorectal cancer.
Interaction of Heavy Metal Lead with Gut Microbiota: Implications for Autism Spectrum Disorder
Authors: Tizabi Yousef , Bennani Samia , Kouhen Nacer El , Getachew Bruk , Aschner Michael 
Published: April 2023

Autism Spectrum Disorder (ASD), a neurodevelopmental disorder characterized by persistent deficits in social interaction and social communication manifests in early childhood and is followed by significant impairment in social and occupational functions in adolescence and adulthood. Although genetics have been implicated, the exact causes of ASD have yet to be fully characterized. New evidence suggests that dysbiosis or perturbation in gut microbiota (GM) and exposure to lead (Pb) may play important roles in ASD etiology. Pb is a toxic heavy metal that has been linked to a wide range of negative health outcomes including anemia, encephalopathy, gastroenteric diseases and more importantly cognitive and behavioral problems inherent to ASD. Pb exposure can disrupt GM, which is essential for maintaining overall health. GM, consisting of trillions of microorganisms, has been shown to play a crucial role in the development of various physiological and psychological functions. GM interacts with the brain in a bidirectional manner referred to as “Gut-Brain Axis (GBA).” In this review, following a general overview of ASD and GM, the interaction of Pb with GM in the context of ASD is emphasized. Potential exploitation of this interaction for therapeutic purposes is also touched upon.
Neonatal Iatrogenic Calcinosis Cutis and Heterotopic Calcification Causing Peripheral Brachial Palsy
Authors: Sakka Samir Akram , Haddad Waleed , Mansor Jeihad 
Published: April 2023

Case:
Iatrogenic heterotopic calcification and calcinosis cutis secondary to extravasations of intravenous calcium is very rare. We report a case of a neonate treated with intravenous calcium resulting in extravasations causing heterotrophic calcification and calcinosis cutis with subcutaneous mass causing perinatal peripheral brachial palsy.

Conclusion:
We highlight these rare potential complications of calcium extravasations and recommend conservative treatment and the avoidance of the temptation of surgical excision of any bony lumps as they resolve spontaneously in time. These complications should not be confused as a fracture with callus formation and periosteal reaction.
Unsuccessful Osteochondral Allograft Cap to Prevent Overgrowth in a Pediatric Patient with Previous Transtibial Amputation
Authors: Pluta Natalia A. , Harrington Colin J. , Smith Douglas G. , Gantsoudes George D. 
Published: April 2023

Case:
This case demonstrates the attempted utilization of an osteochondral allograft for the prevention of bony overgrowth in a patient with fibular hemimelia and previous transtibial amputation with failure of Teflon capping. Additionally, we describe a novel technique to provide additional padding and increase the width of the residual limb using a dermal allograft.

Conclusions:
Bony overgrowth after pediatric amputations is common and often necessitates revision procedures secondary to infection, ulceration, pain, and discomfort with prosthesis use. Our use of an osteochondral allograft cap to prevent bony overgrowth ultimately failed 13 months following the procedure, and further research on various graft options and other treatment modalities is warranted, especially if the proximal fibula is unavailable or there is concern for donor site morbidity associated with harvesting autologous grafts.
Cellular Bone Matrix Leading to Disseminated Tuberculosis After Spinal Fusion
Authors: Issa Tariq Z. , Lambrechts Mark J. , Toci Gregory R. , D'Antonio Nicholas D. , Kanhere Arun P. , Lingenfelter Kenneth , Schroeder Gregory D. , Vaccaro Alexander R. 
Published: April 2023

Case:
Although implanted tuberculosis (TB) is rare, a single lot of cellular bone matrix was found to be infected with TB, leading to devastating outcomes. We present 2 cases referred to our institution because of instrumentation failure caused by TB inoculation of cellular bone matrix.

Conclusion:
Irrespective of spinal region of implanted TB infection, excision of infected bone, extensive irrigation and debridement, and instrumented stabilization are of primary importance to ensure TB eradication and adequate stabilization.
Increasing Quality and Frequency of Goals-of-Care Documentation in the Highest-Risk Surgical Candidates
Authors: Oyekan Anthony A. , Lee Joon Y. , Hodges Jacob C. , Chen Stephen R. , Wilson Alan E. , Fourman Mitchell S. , Clayton Elizabeth O. , Njoku-Austin Confidence , Crasto Jared A. , Wisniewski Mary Kay , Bilderback Andrew , Gunn Scott R. , Levin William I. , Arnold Robert M. , Hinrichsen Katie L. , Mensah Christopher , Hogan MaCalus V. , Hall Daniel E. 
Published: April 2023

Background:
Patient values may be obscured when decisions are made under the circumstances of constrained time and limited counseling. The objective of this study was to determine if a multidisciplinary review aimed at ensuring goal-concordant treatment and perioperative risk assessment in high-risk orthopaedic trauma patients would increase the quality and frequency of goals-of-care documentation without increasing the rate of adverse events.

Methods:
We prospectively analyzed a longitudinal cohort of adult patients treated for traumatic orthopaedic injuries that were neither life- nor limb-threatening between January 1, 2020, and July 1, 2021. A rapid multidisciplinary review termed a “surgical pause” (SP) was available to those who were ≥80 years old, were nonambulatory or had minimal ambulation at baseline, and/or resided in a skilled nursing facility, as well as upon clinician request. Metrics analyzed include the proportion and quality of goals-of-care documentation, rate of return to the hospital, complications, length of stay, and mortality. Statistical analysis utilized the Kruskal-Wallis rank and Wilcoxon rank-sum tests for continuous variables and the likelihood-ratio chi-square test for categorical variables.

Results:
A total of 133 patients were either eligible for the SP or referred by a clinician. Compared with SP-eligible patients who did not undergo an SP, patients who underwent an SP more frequently had goals-of-care notes identified (92.4% versus 75.0%, p = 0.014) and recorded in the appropriate location (71.2% versus 27.5%, p < 0.001), and the notes were more often of high quality (77.3% versus 45.0%, p < 0.001). Mortality rates were nominally higher among SP patients, but these differences were not significant (10.6% versus 5.0%, 5.1% versus 0.0%, and 14.3% versus 7.9% for in-hospital, 30-day, and 90-day mortality, respectively; p > 0.08 for all).

Conclusions:
The pilot program indicated that an SP is a feasible and effective means of increasing the quality and frequency of goals-of-care documentation in high-risk operative candidates whose traumatic orthopaedic injuries are neither life- nor limb-threatening. This multidisciplinary program aims for goal-concordant treatment plans that minimize modifiable perioperative risks.

Level of Evidence:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
A hybrid 1DCNN-GRU-Attention model for three-stage pregnancy pulse identification
Authors: Li Nan , Yu Jiarui , Mao Xiaobo , Zhao Yuping , Huang Luqi 
Published: April 2023

Abstract
Pregnancy pulse diagnosis is an important part of traditional Chinese medicine. In this literature, we propose a hybrid deep learning framework by combining the network advantages of one-dimensional convolutional neural network(1DCNN), gated recurrent unit(GRU) and attention mechanism(ATT), which realized automatic identification and classification of pregnancy period through wrist pulse data. First, the wrist pulse signals of 120 healthy pregnant women in different stages of pregnancy were collected. Second, a hybrid networks for classification in three stages of pregnancy pulse was designed, which is comprised of six 1DCNN blocks and GRU module and attention module. Specifically, the proposed 1DCNN layer are adopted to extract local features from the input wrist pulse data and GRU unit was utilized for overall pulse sequence learning. In addition, the attention mechanism assigned different weights to feature parts according to pulse trends to focus and highlight effective features. Finally, the designed network was used to realize the discrimination of pregnancy pulse. On our local research dataset evaluation, the accuracy of pulse classification of this network for the three stages achieves encouraging results of 93.7%, 93.6%, and 94%, respectively. Furthermore, the model reaches 90.4%, 90.7%, 90.6% and 98% in terms of average precision, sensitivity and F1 score and AUC, respectively. The experiment shows that the designed network has a good reference value for the auxiliary diagnosis of TCM pregnancy pulse diagnosis, which is conducive to promoting the development of modernization of TCM.