bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gender-based heterogeneity of FAHFAs in trained runners
Authors: Nelson Alisa B. , Chow Lisa S. , Dengel Donald R. , Pan Meixia , Hughey Curtis C. , Han Xianlin , Puchalska Patrycja , Crawford Peter A. 
Published: June 2023

AbstractFatty acid esters of hydroxy fatty acid (FAHFA) are anti-diabetic and anti-inflammatory lipokines. Recently FAHFAs were also found to predict cardiorespiratory fitness in trained runners. Here we compared the association between circulating FAHFA baseline concentrations and body composition, determined by dual x-ray absorptiometry, in female runners who were lean (BMI < 25 kg/m2, n = 6), to those who were overweight (BMI ≥ 25 kg/m2, n = 7). We also compared circulating FAHFAs in lean male runners (n = 8) to the same trained lean female (n = 6) runner group. Circulating FAHFAs were increased in females in a manner that was modulated by specific adipose depot sizes, blood glucose, and lean body mass. As expected, circulating FAHFAs were diminished in the overweight group, but, strikingly, in both lean and overweight cohorts, increases in circulating FAHFAs were promoted by increased fat mass, relative to lean mass. These studies suggest multimodal regulation of circulating FAHFAs and raise hypotheses to test endogenous FAHFA dynamic sources and sinks in health and disease, which will be essential for therapeutic target development. Baseline circulating FAHFA concentrations could signal sub-clinical metabolic dysfunction in metabolically healthy obesity.
Brewing COFFEE: A sequence-specific coarse-grained energy function for simulations of DNA-protein complexes
Authors: Chakraborty Debayan , Mondal Balaka , Thirumalai D. 
Published: June 2023

AbstractDNA-protein interactions are pervasive in a number of biophysical processes ranging from transcription, gene expression, to chromosome folding. To describe the structural and dynamic properties underlying these processes accurately, it is important to create transferable computational models. Toward this end, we introduceCoarse grainedforcefield forenergyestimation, COFFEE, a robust framework for simulating DNA-protein complexes. To brew COFFEE, we integrated the energy function in the Self-Organized Polymer model with Side Chains for proteins and the Three Interaction Site model for DNA in a modular fashion, without re-calibrating any of the parameters in the original force-fields. A unique feature of COFFEE is that it describes sequence-specific DNA-protein interactions using a statistical potential (SP) derived from a dataset of high-resolution crystal structures. The only parameter in COFFEE is the strength (λDNAPRO) of the DNA-protein contact potential. For an optimal choice ofλDNAPRO, the crystallographic B-factors for DNA-protein complexes, with varying sizes and topologies, are quantitatively reproduced. Without any further readjustments to the force-field parameters, COFFEE predicts the scattering profiles that are inquantitative agreementwith SAXS experiments as well as chemical shifts that are consistent with NMR. We also show that COFFEE accurately describes the salt-induced unraveling of nucleosomes. Strikingly, our nucleosome simulations explain the destabilization effect of ARG to LYS mutations, which does not alter the balance of electrostatic interactions, but affects chemical interactions in subtle ways. The range of applications attests to the transferability of COFFEE, and we anticipate that it would be a promising framework for simulating DNA-protein complexes at the molecular length-scale.Graphical TOC Entry
Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells
Authors: Xue Liang , Spahn Christian M.T. , Schacherl Magdalena , Mahamid Julia 
Published: June 2023

AbstractRibosome-targeting antibiotics represent an important class of antimicrobial drugs. Chloramphenicol (Cm) is a well-studied peptidyl transfer center (PTC) binder, and growing evidence suggests it inhibits translation in a nascent peptide sequence-dependent manner. How such inhibition on the molecular scale translates to action on the cellular level remains unclear. Here, we employ cryo-electron tomography to visualize the impact of Cm inside the bacteriumMycoplasma pneumoniae. By resolving cellular Cm-bound ribosomes to 3.0 Å, we provide atomic detail on Cm’s coordination and interaction with natural nascent peptides and tRNAs in the PTC. We find that Cm leads to accumulation of translation elongation states that indicate ongoing futile accommodation cycles, and to extensive ribosome collisions. We thus suggest that beyond its inhibition of protein synthesis, the action of Cm may involve activation of cellular stress responses. This work exemplifies how in-cell structural biology advances understanding of mechanisms of action for extensively-studied antibiotics.
otpbfunctions in a Lef1-dependent transcriptional network required for expression of the stress response inhibitorcrhbpin the zebrafish hypothalamus
Authors: Figueroa Priscilla , Cheng Jennifer , Wang Guangning , Kartchner Cade , Brito de Andrade Debora , Watters Harrison , Crispell Ethan , Dorsky Richard I. 
Published: June 2023

AbstractThe vertebrate hypothalamus regulates physiological and behavioral responses to environmental stimuli through the function of evolutionarily-conserved neuronal subpopulations. Our previous work found that mutation of zebrafishlef1, which encodes a transcriptional mediator of the Wnt signaling pathway, leads to the loss of hypothalamic neurons and behavioral phenotypes that are both associated with stress-related human mood disorders However, the specific Lef1 target genes that link neurogenesis to behavior remain unknown. One candidate isotpb, which encodes a transcription factor with known roles in hypothalamic development. Here we show thatotpbexpression in the posterior hypothalamus is Lef1-dependent, and that likelef1, its function is required for the generation ofcrhbp+ neurons in this region. Transgenic reporter analysis of acrhbpconserved noncoding element suggests thatotpbparticipates in a transcriptional regulatory network with other Lef1 targets. Finally, consistent with a role forcrhbpin inhibiting the stress response, zebrafishotpbmutants exhibit decreased exploration in a novel tank diving assay. Together our findings suggest a potential evolutionarily-conserved mechanism for the regulation of innate stress response behaviors through Lef1-mediated hypothalamic neurogenesis.
Fusion pore dynamics of large secretory vesicles define a distinct mechanism of exocytosis
Authors: Biton Tom , Scher Nadav , Carmon Shari , Elbaz-Alon Yael , Schejter Eyal D. , Shilo Ben-Zion , Avinoam Ori 
Published: June 2023

AbstractExocrine cells utilize large secretory vesicles (LSVs) up to 10 μm in diameter. LSVs fuse with the apical surface, often recruiting actomyosin to extrude their content through dynamic fusion pores. The molecular mechanism regulating pore dynamics remains largely uncharacterized. We observe that the fusion pore of LSVs in the Drosophila larval salivary glands expand, stabilize, and constrict. Arp2/3 is essential for pore expansion and stabilization, while myosin II is essential for pore constriction. We identify several Bin-Amphiphysin-Rvs (BAR) homology domain proteins that regulate fusion pore expansion and stabilization. We show that the I-BAR protein Missing-in-Metastasis (MIM) localizes to the fusion site and is essential for pore expansion and stabilization. The MIM I-BAR domain is essential but not sufficient for localization and function. We conclude that MIM acts in concert with actin, myosin II, and additional BAR-domain proteins to control fusion pore dynamics, mediating a distinct mode of exocytosis that facilitates actomyosin-dependent content release that maintains apical membrane homeostasis during secretion.
Acvr1bloss increases formation of pancreatic precancerous lesions from acinar and ductal cells of origin
Authors: Saeki Kiyoshi , Wood Ian S. , Wang Wei Chuan K. , Patil Shilpa , Sun Yanping , Schaeffer David F. , Su Gloria H. , Kopp Janel L. 
Published: June 2023

AbstractBackground & AimsPancreatic ductal adenocarcinoma (PDAC) can develop from precursor lesions, including pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasm (IPMN). Previous studies indicated that loss ofAcvr1baccelerates the Kras-mediated development of papillary IPMN in the mouse pancreas, however, the cell type predominantly affected by these genetic changes remains unclear.MethodsWe investigated the contribution of cellular origin by inducing IPMN associated mutations-KRASG12Dexpression andAcvr1bloss - specifically in acinar (Ptf1aCreER;KrasLSL-G12D;Acvr1bfl/flmice) or ductal (Sox9CreER;KrasLSL-G12D;Acvr1bfl/flmice) cells in mice. We then performed MRI imaging and a thorough histopathological analysis of their pancreatic tissues.ResultsThe loss ofAcvr1bincreased the development of PanIN and IPMN-like lesions when either acinar and ductal cells expressed a Kras mutation. MRI, immunohistochemistry and histology revealed large IPMN-like lesions in these mice that exhibited features of flat, gastric epithelium. In addition, cyst formation in both mouse models was accompanied by chronic pancreatitis. Experimental acute pancreatitis accelerated the development of large mucinous cysts and PanIN when acinar, but not ductal, cells expressed mutant Kras and lostAcvr1b.ConclusionThese findings indicate that loss ofAcvr1bin the presence of the Kras oncogene promotes the development of large and small precancerous lesions from both ductal and acinar cells. However, the IPMN-like phenotype was not equivalent to that observed when these mutations were made in all pancreatic cells during development. Our study underscores the significance of the cellular context in the initiation and progression of precursor lesions from exocrine cells.
Identification and characterisation ofPlanococcus citri cis- andtrans-isoprenyl diphosphate synthase genes, supported by short- and long-read transcriptome data
Authors: Juteršek Mojca , Gerasymenko Iryna M. , Petek Marko , Haumann Elisabeth , Vacas Sandra , Kallam Kalyani , Gianoglio Silvia , Navarro-Llopis Vicente , Fuertes Ismael Navarro , Patron Nicola , Orzáez Diego , Gruden Kristina , Warzecha Heribert , Baebler Špela 
Published: June 2023

AbstractMany insect species rely on diverse terpenoids for their development and interorganismal interactions. However, little is known about terpenoid biosynthesis in insects. The monoterpenoid sex pheromones of mealybugs and scale insects (Coccoidea) are particularly enigmatic, with several species producing unique structures presumed to result from the irregular coupling activity of unidentified isopentenyl diphosphate synthases (IDSs). Enzymes capable of similar transformations have previously only been described from a few plant, bacterial and archaeal species. To investigate if insect irregular monoterpenes can be biosynthesised by similar enzymes, we performed a comprehensive search for IDS coding sequences in the genome ofPlanococcus citri, a widespread agricultural pest. We complemented the availableP. citrigenome data with newly generated short- and long-read transcriptome data. The identified candidate genes had homology to both short- and long-chain IDSs and some appeared to be paralogous, indicating gene duplications and consequent IDS gene family expansion inP. citri. We tested the activity of eleven candidate gene products, confirmingin vitroregular activity for five enzymes, one of which (transIDS5) also produced the irregular prenyl diphosphates, maconelliyl and lavandulyl diphosphate. Targeted mutagenesis of selected aspartates and a lysine in the active site oftransIDS5 uncovered their importance for chain-length preference and irregular coupling. This work provides an important foundation for deciphering terpenoid biosynthesis in mealybugs, as well as a potential source of enzymes for the biotechnological production of sustainable insect pest management products.
Localisation of PPE manufacturing during COVID-19 (local manufacturing partnership and Kingsgate Clothing South Africa)
Authors: Scheepers Caren Brenda , Mathibe Motshedisi Sina , Verachia Abdullah 
Published: June 2023

Learning outcomes
• After working through the case and assignment questions, students will be able to do the following:• Identify the difference between core competencies and dynamic capabilities and how they make a difference in a crisis towards building an agile organisation.• Evaluate the support through a collaborative, temporary, trans-organisational system to local manufacturers to achieve agility and sustainability.• Realise the importance of clear expectations exchange and communication between partners to enhance collaboration, even in temporary structures in increasing agility and sustainability.

Case overview/synopsis
The COVID-19 pandemic and subsequent lockdowns created a crisis for South Africa and the President, Cyril Ramaphosa called for urgent collaboration between business, labour and government to meet the demand for locally produced Personal Protective Equipment (PPE) and medical devices. The case illustrates this response and collaboration between government, labour and business through a temporary newly formed structure, called Business for South Africa (B4SA). Ross Boyd, Head of the innovation work stream of B4SA which housed the local manufacturing partnership (LMP) was considering his dilemma of how to be agile in sustaining their support to the South African manufacturers even though the LMP was a temporary structure. The local manufacturers had to repurpose their production lines to produce local PPE and medical devices. How could the LMP support the South African manufacturers to sustain their agility in building capabilities during COVID-19? At the same time, Ahmed Dhai, the Group Executive of Operations of Kingsgate Clothing, which was benefiting from the support of the LMP, was reflecting on his leadership in taking decisions to repurpose production and increase capacity during COVID-19. Dhai was considering his dilemma of being agile during and beyond the COVID-19 pandemic. The case features several decisions taken by Kingsgate and offers students the opportunity to evaluate these decisions given the fluctuations in supply and demand of PPE and the leadership that Dhai demonstrated in how he communicated and dealt with his staff during the pandemic. Students could also give recommendations to Ross Boyd and Ahmed Dhai on how they could lead their organisations to be more agile during and beyond COVID-19.

Complexity academic level
The case study is suitable for MBA or MPhil level on Strategy courses. The case would also find good application in Organisational Behaviour and Leadership courses on Masters level and Executive Education programmes.

Supplementary material
Teaching notes are available for educators only.

Subject code
CSS 7: Management Science.
Integration of sustainability in the curricula of public higher education institutions in Portugal: do strategic plans and self-report align?
Authors: Duarte Marina , Caeiro Sandra Sofia , Farinha Carla Sofia , Moreira Ana , Santos-Reis Margarida , Rigueiro Constança , Simão João 
Published: June 2023

Purpose
This study aims to explore the alignment between strategic plans of the Portuguese public higher education institutions (HEIs) and their perception of the integration of sustainability in education and curricula.

Design/methodology/approach
The strategic plans from 15 institutions were selected for content analysis; data about the integration of sustainability in education and curricula, from these HEI, were collected with an online questionnaire (self-report survey). Qualitative and quantitative analyses were performed.

Findings
Strategic plans of the Portuguese public HEIs seem to not be sufficiently aligned with self-assessment integration of sustainability in education and curricula.

Research limitations/implications
The classifications used in the content analysis were constructed and revised by the authors to reduce coder interpretation issues and subsequent bias in the results. However, some subjectivity could remain. The analysis of strategic plans and self-report surveys answered by top management, or a technician, does not assess the practices and sustainability implementation in education and curricula.

Practical implications
This study allows the self-report of already-implemented practices to be compared to the planned strategy of HEI governance in Portugal as stated in their strategic plans.

Originality/value
An analysis and respective insights on the lack of connection between strategic planning and self-report practices about sustainability implementation, using Portugal as a case study.
Collective case study: integrated health and social care for older adults within a Canadian context
Authors: Cheng Siu Mee , Catallo Cristina 
Published: June 2023

PurposeCanada's population is aging and there are concerns that the welfare system may not support the increased demands on it. Integrated health and social care (IHSC) produces positive health and system outcomes but it needs to be better understood within a Canadian context. The purpose of this collective case study of three IHSC initiatives in Alberta, Ontario and Nova Scotia was to determine the factors that support successful services integration among different healthcare and social services organizations serving older adults within a Canadian context.Design/methodology/approachThis study used the Cheng and Catallo (2020) IHSC conceptual framework (CF) to guide the research. Primary data were based on key informant interviews of representatives from organizations that comprised each case and focus groups. A cross-case analysis was undertaken to determine common themes.FindingsThe cross-case analysis revealed that the three cases shared common integration and external influence factors based on the Cheng and Catallo (2020) CF. Some new factors were identified.Originality/valueThe study revealed that the Canadian context was important in influencing integration in the three cases and that there is a unique Canadian aspect to IHSC. The study offers up practical insights for government leaders and service administrators to improve IHSC for older adults. The study also identifies how the Cheng and Catallo (2020) IHSC CF can be enhanced and points to research opportunities to test the framework.
Explaining the visual attention model in impulsive purchase behavior of tourism industry customers by theme analysis method
Authors: Farokhi Sajad , Namamian Farshid , Asghari Sarem Ali , Ghobadi Lamuki Tohfe 
Published: June 2023

Purpose
This study aims to explain the model of impulsive purchase behavior in the Islamic Republic of Iran’s tourism industry.

Design/methodology/approach
This study uses the inductive–deductive approach for data collection. Field (interviews) and library (articles, books and theses) methods have been used for data collection. After determining the research variables, content analysis units (theme, category and markers) are specified. Academic experts and professionals in the subject area helped during the model design stage.

Findings
The results reveal 14 visual attention variables which are classified into four categories: attractiveness in the market, emotional reactions, improving the shopping style of tourists and visual advertising.

Research limitations/implications
This study focuses only on Iran. There is scope to expand the discussion with more interviews. The impact of the COVID-19 pandemic on tourism presented some unique challenges in participant recruitment. Future studies could focus on other Middle Eastern countries or other international areas. Moreover, future researchers could analyze other variables affecting impulsive purchase.

Originality/value
No other studies on tourism industry marketing have recognized the effective variables in impulsive purchase. To fill this gap, this paper explains the visual attention model in tourists’ impulsive purchase behavior.
Monetary income as opportunity cost: exploring the negative effect on free knowledge contribution of knowledge suppliers
Authors: Jiang Shan , Nguyen Duc Khuong , Dai Peng-Fei , Meng Qingxin 
Published: June 2023

Purpose
In the hybrid knowledge-sharing platform where paid and nonpaid (“free”) knowledge activities coexist, users’ free knowledge contribution may be influenced by financial factors. From the perspective of opportunity cost, this study investigates the direct effect of how the amount of monetary income from users’ contribution to paid knowledge activities influences their free knowledge contribution behavior in the future. Further, this study aims to verify the interaction effect of financial and nonfinancial factors (i.e. the experience of free knowledge contribution and social recognition) on free knowledge contribution.

Design/methodology/approach
Objective data was collected from a hybrid knowledge-sharing platform in China and then analyzed by using zero-inflated negative binomial regression model.

Findings
Results show that the amount of monetary income that knowledge suppliers gain from paid knowledge contribution negatively influences their free knowledge contribution. Experience of free knowledge contribution strengthens the negatively main effect, while social recognition has the weakening moderating role.

Originality/value
Although some studies have explored and verified the positive spillover effect of financial incentives on free knowledge contribution, the quantity dimension is ignored. This study examines the hindering influence of the quantity of monetary income from the perspective of opportunity cost. By taking the characteristic of knowledge suppliers and platforms as moderators, this study deepens the understanding of the influence of monetary income on free knowledge contribution in the hybrid knowledge-sharing platform.
Money supply, inflation and economic growth of Sri Lanka: co-integration and causality analysis
Authors: Madurapperuma Wasanthi 
Published: June 2023

PurposeGDP growth, money growth and inflation are essential to an economy's macroeconomic stability and have a direct impact on the policymaking process. Sri Lanka is currently concerned about high inflation. Inflation is a monetary phenomenon. Inflation has been caused by monetary policy in several nations. According to the economic theories of Karl Marx, Irving Fisher and Milton Friedman, a continuous increase in the money supply causes inflation. This paper aims to investigate the relationship between Sri Lanka's GDP growth, money growth and inflation.Design/methodology/approachAn econometric model and the economic theories of Fisher and Friedman are used to figure out how money supply, inflation and economic growth are linked. Between 1990 and 2021, data were gathered from secondary sources.FindingsThe increase in the money supply is found to cause inflation. Inflation has negative effects on both short- and long-term economic growth. Long-term, the increase in money supply has a negative effect on economic growth.Research limitations/implicationsAccording to research, the money supply and inflation are inextricably linked, and the money supply has a direct impact on economic growth. As a result, the government should have an appropriate monetary policy and proposals to control inflation levels and stimulate economic growth.Originality/valueThe paper adds to the existing literature in two ways. First, it fills in the lack of studies in Sri Lanka, where there are no papers on this important relationship, especially with a modern econometric study. Second, it tries to shed light on the asymmetric shocks (both positive and negative shocks and changes) between the three variables, which was not done in previous studies.