bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radio-continuum decrements associated to shadowing from the central warp in transition disc DoAr 44
Authors: Arce-Tord Carla , Casassus Simon , Dent William R F , Pérez Sebastián , Cárcamo Miguel , Weber Philipp , Engler Natalia , Cieza Lucas A , Hales Antonio , Zurlo Alice , Marino Sebastian 
Published: September 2023

ABSTRACT
Warps have often been used to explain disc properties, but well-characterized examples are important due to their role in disc evolution. Scattered light images of discs with central gaps have revealed sharp warps, such that the outer rings are shadowed by tilted inner discs. The near-infrared intensity drops along the ring around T-Tauri star DoAr 44 have been interpreted in terms of a central warp. We report new ALMA observations of DoAr 44 in the continuum at 230 and 350 GHz (at ∼10 au), along with a new epoch of Spectro Polarimetric High-contrast Exoplanet REsearch (SPHERE)/Infrared Dual-band Imager and Spectrograph differential polarized imaging taken during excellent weather conditions. The ALMA observations resolve the ring and confirm the decrements proposed from deconvolution of coarse 336 GHz data. The scattered light image constrains the dips, which correspond to a misaligned inner disc with a relative inclination ξ = 21.4 $^{+6.7}_{-8.3}$ deg. The SPHERE intensity profile shows a morphological change compared to a previous epoch that may be interpreted as a variable orientation of the inner disc, from ξ ∼ 30 deg to ξ ∼ 20 deg. The intensity dips probably correspond to temperature decrements, as their mm-spectral index, $\alpha ^{230 \textrm {GHz}}_{350 \textrm {GHz}} \sim$ 2.0 ± 0.1, is indicative of optically thick emission. The azimuth of the two temperature decrements are leading clockwise relative to the infrared-dips, by η = 14.95 deg and η = 7.92 deg. For a retrograde disc, such shifts are expected from a thermal lag and imply gas surface densities of Σg = 117 ± 10 g cm−2 and Σg = 48 ± 10 g cm−2. A lopsided disc, with contrast ratio fr = 2.4 ± 0.5, is also consistent with the large continuum crescent.
Electron-impact single ionization for N+ ion
Authors: Jonauskas Valdas 
Published: September 2023

ABSTRACT
Electron-impact single ionization from levels of the ground configuration of the N+ ion is presented. Study analyses direct and indirect processes of the ionization. Convergence of the excitation-autoionization cross-sections is ensured by taking excitations up to shells with the principal quantum numbers n ≤ 10. The potential of the ionizing ion is used in the study. The scaled distorted wave cross-sections show a fairly good agreement with measurements. The study shows a presence of metastable states in the ion beams of experiments.
Unfolding of an RNA G-quadruplex motif in the negative strand genome of porcine reproductive and respiratory syndrome virus by host and viral helicases to promote viral replication
Authors: Fang Puxian , Xie Congbao , Pan Ting , Cheng Ting , Chen Wei , Xia Sijin , Ding Tong , Fang Junkang , Zhou Yanrong , Fang Liurong , Wei Dengguo , Xiao Shaobo 
Published: September 2023

Abstract
G-quadruplex (G4) is a unique secondary structure formed by guanine-rich nucleic acid sequences. Growing studies reported that the genomes of some viruses harbor G4 structures associated with viral replication, opening up a new field to dissect viral infection. Porcine reproductive and respiratory syndrome virus (PRRSV), a representative member of Arteriviridae, is an economically significant pathogen that has devastated the swine industry worldwide for over 30 years. In this study, we identified a highly conserved G-rich sequence with parallel-type G4 structure (named PRRSV-G4) in the negative strand genome RNA of PRRSV. Pyridostatin (PDS), a well-known G4-binding ligand, stabilized the PRRSV-G4 structure and inhibited viral replication. By screening the proteins interacting with PRRSV-G4 in PRRSV-infected cells and single-molecule magnetic tweezers analysis, we found that two helicases, host DDX18 and viral nsp10, interact with and efficiently unwound the PRRSV-G4 structure, thereby facilitating viral replication. Using a PRRSV reverse genetics system, we confirmed that recombinant PRRSV with a G4-disruptive mutation exhibited resistance to PDS treatment, thereby displaying higher replication than wild-type PRRSV. Collectively, these results demonstrate that the PRRSV-G4 structure plays a crucial regulatory role in viral replication, and targeting this structure represents a promising strategy for antiviral therapies.
Distinct RPA functions promote eukaryotic DNA replication initiation and elongation
Authors: Pike Alexandra M , Friend Caitlin M , Bell Stephen P 
Published: September 2023

Abstract
Replication protein A (RPA) binds single-stranded DNA (ssDNA) and serves critical functions in eukaryotic DNA replication, the DNA damage response, and DNA repair. During DNA replication, RPA is required for extended origin DNA unwinding and DNA synthesis. To determine the requirements for RPA during these processes, we tested ssDNA-binding proteins (SSBs) from different domains of life in reconstituted Saccharomyces cerevisiae origin unwinding and DNA replication reactions. Interestingly, Escherichia coli SSB, but not T4 bacteriophage Gp32, fully substitutes for RPA in promoting origin DNA unwinding. Using RPA mutants, we demonstrated that specific ssDNA-binding properties of RPA are required for origin unwinding but that its protein-interaction domains are dispensable. In contrast, we found that each of these auxiliary RPA domains have distinct functions at the eukaryotic replication fork. The Rfa1 OB-F domain negatively regulates lagging-strand synthesis, while the Rfa2 winged-helix domain stimulates nascent strand initiation. Together, our findings reveal a requirement for specific modes of ssDNA binding in the transition to extensive origin DNA unwinding and identify RPA domains that differentially impact replication fork function.
The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation
Authors: Wang Yilin , Risteski Patrik , Yang Yang , Chen Huan , Droby Gaith , Walens Andrea , Jayaprakash Deepika , Troester Melissa , Herring Laura , Chernoff Jonathan , Tolić Iva M , Bowser Jessica , Vaziri Cyrus 
Published: September 2023

Abstract
Stringent control of centrosome duplication and separation is important for preventing chromosome instability. Structural and numerical alterations in centrosomes are hallmarks of neoplastic cells and contribute to tumorigenesis. We show that a Centrosome Amplification 20 (CA20) gene signature is associated with high expression of the Tripartite Motif (TRIM) family member E3 ubiquitin ligase, TRIM69. TRIM69-ablation in cancer cells leads to centrosome scattering and chromosome segregation defects. We identify Serine/threonine-protein kinase 3 (MST2) as a new direct binding partner of TRIM69. TRIM69 redistributes MST2 to the perinuclear cytoskeleton, promotes its association with Polo-like kinase 1 (PLK1) and stimulates MST2 phosphorylation at S15 (a known PLK1 phosphorylation site that is critical for centrosome disjunction). TRIM69 also promotes microtubule bundling and centrosome segregation that requires PRC1 and DYNEIN. Taken together, we identify TRIM69 as a new proximal regulator of distinct signaling pathways that regulate centrosome dynamics and promote bipolar mitosis.
The arginine methyltransferase Prmt1 coordinates the germline arginine methylome essential for spermatogonial homeostasis and male fertility
Authors: Azhar Muhammad , Xu Caoling , Jiang Xue , Li Wenqing , Cao Yuzhu , Zhu Xiaoli , Xing Xuemei , Wu Limin , Zou Jiaqi , Meng Lan , Cheng Yu , Han Wenjie , Bao Jianqiang 
Published: September 2023

Abstract
Arginine methylation, catalyzed by the protein arginine methyltransferases (PRMTs), is a common post-translational protein modification (PTM) that is engaged in a plethora of biological events. However, little is known about how the methylarginine-directed signaling functions in germline development. In this study, we discover that Prmt1 is predominantly distributed in the nuclei of spermatogonia but weakly in the spermatocytes throughout mouse spermatogenesis. By exploiting a combination of three Cre-mediated Prmt1 knockout mouse lines, we unravel that Prmt1 is essential for spermatogonial establishment and maintenance, and that Prmt1-catalyzed asymmetric methylarginine coordinates inherent transcriptional homeostasis within spermatogonial cells. In conjunction with high-throughput CUT&Tag profiling and modified mini-bulk Smart-seq2 analyses, we unveil that the Prmt1-deposited H4R3me2a mark is permissively enriched at promoter and exon/intron regions, and sculpts a distinctive transcriptomic landscape as well as the alternative splicing pattern, in the mouse spermatogonia. Collectively, our study provides the genetic and mechanistic evidence that connects the Prmt1-deposited methylarginine signaling to the establishment and maintenance of a high-fidelity transcriptomic identity in orchestrating spermatogonial development in the mammalian germline.
circAtlas 3.0: a gateway to 3 million curated vertebrate circular RNAs based on a standardized nomenclature scheme
Authors: Wu Wanying , Zhao Fangqing , Zhang Jinyang 
Published: September 2023

Abstract
Recent studies have demonstrated the important regulatory role of circRNAs, but an in-depth understanding of the comprehensive landscape of circRNAs across various species still remains unexplored. The current circRNA databases are often species-restricted or based on outdated datasets. To address this challenge, we have developed the circAtlas 3.0 database, which contains a rich collection of 2674 circRNA sequencing datasets, curated to delineate the landscape of circRNAs within 33 distinct tissues spanning 10 vertebrate species. Notably, circAtlas 3.0 represents a substantial advancement over its precursor, circAtlas 2.0, with the number of cataloged circRNAs escalating from 1 007 087 to 3 179 560, with 2 527 528 of them being reconstructed into full-length isoforms. circAtlas 3.0 also introduces several notable enhancements, including: (i) integration of both Illumina and Nanopore sequencing datasets to detect circRNAs of extended lengths; (ii) employment of a standardized nomenclature scheme for circRNAs, providing information of the host gene and full-length circular exons; (iii) inclusion of clinical cancer samples to explore the biological function of circRNAs within the context of cancer and (iv) links to other useful resources to enable user-friendly analysis of target circRNAs. The updated circAtlas 3.0 provides an important platform for exploring the evolution and biological implications of vertebrate circRNAs, and is freely available at http://circatlas.biols.ac.cn and https://ngdc.cncb.ac.cn/circatlas.
Solar horizontal flow evaluation using neural network and numerical simulations with snapshot data
Authors: Masaki Hiroyuki , Hotta Hideyuki , Katsukawa Yukio , Ishikawa Ryohtaroh T 
Published: September 2023

Abstract
We suggest a method that evaluates the horizontal velocity in the solar photosphere with easily observable values using a combination of neural network and radiative magnetohydrodynamics simulations. All three-component velocities of thermal convection on the solar surface have important roles in generating waves in the upper atmosphere. However, the velocity perpendicular to the line of sight (LoS) is difficult to observe. To deal with this problem, the local correlation tracking (LCT) method, which employs the difference between two images, has been widely used, but this method has several disadvantages. We develop a method that evaluates the horizontal velocity from a snapshot of the intensity and the LoS velocity with a neural network. We use data from numerical simulations for training the neural network. While two consecutive intensity images are required for LCT, our network needs just one intensity image at only a specific moment for input. From these input arrays, our network outputs a same-size array of a two-component velocity field. With only the intensity data, the network achieves a high correlation coefficient between the simulated and evaluated velocities of 0.83. In addition, the network performance can be improved when we add LoS velocity for input, enabling us to achieve a correlation coefficient of 0.90. Our method is also applied to observed data.
L’évaluation et la prise en charge de l’énurésie en pédiatrie générale
Authors: Harris James , Lipson Alisa , Dos Santos Joana 
Published: September 2023

Résumé
Pour évaluer l’énurésie, un trouble pédiatrique courant, il faut en distinguer la forme monosymptomatique de la forme non monosymptomatique et établir la présence d’affections concomitantes. La prise en charge simultanée des facteurs cooccurrents est le meilleur moyen pour obtenir un résultat satisfaisant. Le traitement commence par l’éducation du patient et de sa famille sur l’évolution naturelle de l’énurésie et par des conseils pratiques sur le comportement. Les données probantes en appui à des interventions particulières sont limitées, et les enfants et les familles devraient participer au choix du traitement approprié. Les dispositifs d’alarme contre l’énurésie et la desmopressine représentent des possibilités thérapeutiques lorsqu’une intervention plus active est souhaitée. Des améliorations cliniques et des traitements combinés sont en voie de se dégager.
4 Adolescents Identifying their Barriers to Access Mental Health and Addiction Services in a Small Rural Canadian Town
Authors: Ortiz-Alvarez Oliva , Cozzi Regina , Blackburn Heather , Marmura Hana 
Published: September 2023

Abstract

Background
Mental health illnesses or disorders and addictions are common in the Canadian population. Despite the huge burden associated with child and adolescent mental health disorders around the world, in Canada and in our local communities there are gaps in the provision of care for this population. Teenagers are particularly vulnerable to the inadequate provision of care, as services have been traditionally conceptualized to serve the needs of children and adults. The gap between need and access to mental health and addiction services has been identified by: parents, teachers, and health care providers, albeit mostly studied in large urban populations.

Objectives
The purpose of the study was to identify the adolescent-perceived barriers to accessing mental health services in the community.

Methods
We conducted a cross-sectional study in a small rural town through online questionnaires, asking local high school students to report their perceived personal and community level barriers to accessing services. The sample included 244 high school students answering the questionnaire, which included sections on perception of individual level barriers and perception of community level barriers. We also captured respondent characteristics, including demographics, attempts to access mental health services in the past, and awareness of resources.

Results
Respondents’ top perceived barriers were the lack of awareness regarding available resources, lack of proper education regarding mental health issues, and uncertainty of what the treatment would entail. Students who had previously accessed services identified the lack of qualified mental health professionals, frequent change of mental health professionals, and lack of collaboration and communication among service providers as their top barriers, whereas students who had not accessed services reported the uncertainty of the treatment, fear of social stigma, and exclusion as primary barriers.

Conclusion
The study findings strongly support the need for promoting better mental health literacy in the school and in the community as well as improving the services available. A critical look at the mental health curriculum components across the grades is urgently needed. Planning mental health services addressing these perceived barriers might improve teenagers’ access to services, allowing those in need to seek help and receive effective treatment before they are in crisis.

Potential competing interests
Received funding from MicroResearch and Saint Martha's Foundation
10 Paediatricians’ Awareness of Canadian Screen Time Guidelines, Perception of Screen Time Use, and Counseling during the COVID-19 Pandemic
Authors: Amos Kiersten , Ens Andrea , Ogilvie Jacqueline , Ponti Michelle , Miller Michael , Yang Fanyu 
Published: September 2023

Abstract

Background
Paediatricians are essential for guiding families on screen time use as digital media become increasingly prevalent, particularly through the COVID-19 pandemic. However, there is no literature on paediatrician awareness of Canadian guidelines or on perception of these guidelines during the pandemic.

Objectives
The aim of this study was to assess the knowledge, attitudes, and comfort of paediatricians with the Canadian Paediatric Society’s (CPS) screen time guidelines, specifically during the COVID-19 pandemic.

Design/Methods
Our survey was developed by a paediatric resident and paediatric endocrinologist, reviewed by local experts, and sent electronically to members of the Community and Developmental Paediatrics Sections of the CPS.

Results
All 53 respondents were aware of current CPS screen time guidelines, and the majority had fair to excellent knowledge of guidance for both age groups (&lt;5 years, and school-aged children and adolescents). Over 80% noticed increased screen time use during the pandemic, and 98% were somewhat or very concerned about screen time use and their patients’ health or well-being. Qualitative data indicated paediatricians were concerned about associations between increased screen time with worsening behaviour, mental health concerns, obesity, and sedentary lifestyle. The greatest implementation barrier was perceived to be insufficient motivation or support from caregivers/families.

Conclusion
Our study demonstrates that paediatricians are highly knowledgeable and comfortable with current screen time guidelines, and frequently counsel their patients accordingly. Despite this, paediatricians are increasingly concerned with negative health outcomes associated with screen time use. We hope these results empower paediatricians to continue counseling patients and encourage further local advocacy and public education around increased screen time and related health risks in children.
11 Experiences of Inpatient Healthcare Services among Children with Medical Complexity: A Scoping Review
Authors: Asaad Lauren , Dewan Tammie , MacKay Lyndsay , Buchanan Francine , Hayden Alix , Montgomery Lara 
Published: September 2023

Abstract

Background
Children with medical complexity (CMC) have among the highest need for inpatient services in paediatrics. In addition to effectiveness and safety, experience of care (EoC) is a crucial pillar of health care quality, encompassing all interactions that shape a patient/family’s perception of care. Current evidence suggests that CMC have lower EoC compared to other populations. Collating the existing cross-disciplinary evidence will identify critical aspects of their EoC, both positive and negative, that could inform the development and evaluation of health services interventions for CMC in the hospital setting.

Objectives
The objectives of this scoping review were to map the existing literature related to the experience of inpatient health services for CMC and their families, describe research methods, and categorize key concepts of EoC while simultaneously identifying knowledge gaps.

Design/Methods
The Joanna Briggs Institute (JBI) methodology for scoping reviews was used. Articles related to CMC and the inpatient setting were identified through EMBASE, CINAHL, Web of Science, MEDLINE and PsychoInfo. Reference lists were also searched for relevant articles. Screening of titles, abstracts, and full-text articles was conducted by two independent researchers, resolving discrepancies by discussion and consensus. Data extraction and critical appraisal for included papers were likewise conducted by two separate researchers. Abstracted data included authors, participants, objectives, methodology, and key findings. Critical appraisal was conducted using the Mixed Methods Appraisal Tool and the JBI critical appraisal tool for systemic reviews.

Results
A total of 4463 separate articles underwent title/abstract review and 513 went on to full text review. After applying inclusion and exclusion criteria, a total of 48 articles was included in the review. There were 26 qualitative studies, 8 quantitative studies, 7 mixed methods studies, and 7 review papers. Quantitative studies used a variety of instruments to measure EoC, with the most common being the Measures of Processes of Care. The primary concepts related to EoC to be identified include partnerships (including continuity, building a therapeutic alliance), communication (particularly parent-nurse), decision-making and goal setting, and psychological distress and symptoms that occur during a hospitalization.

Conclusion
This review synthesizes the existing evidence on inpatient EoC for CMC. Although a diverse set of elements related to EoC are described, there are few interventional studies that include these as outcome measures. These EoC elements could be mapped to existing instruments or form the basis for the development of new tools that would allow the incorporation of EoC into evaluation of inpatient interventions.
16 Autistic Perspectives on the Acceptability of Paediatric Autism Therapies
Authors: Caven Isabelle , Penner Melanie 
Published: September 2023

Abstract

Background
Autistic children and their families access services, such as occupational therapy (OT), speech language pathology (SLP) services, applied behaviour analysis (ABA)-based therapies and physiotherapy (PT). There are varying levels of evidence supporting the efficacy of these therapies, and some autistic-led advocacy is critical of the methods and aims of certain services. Exploring stakeholder perspectives is critical to ensure services are appropriate and informed by lived experiences. This work is part of a larger survey that explored the perspectives of autistic individuals, parents of autistic children, and clinicians on these services.

Objectives
This study explored how autistic people perceive the acceptability of OT, SLP services, ABA-based therapies, and PT.

Design/Methods
Using the Theoretical Framework of Acceptability (TFA), a survey was built with input from autistic people, parents of autistic children, and clinicians and shared online through social media and autism organizations. Demographic data and perspectives were captured. An acceptability sub-score was produced for each therapy, ranging from 0 (least accepting) to 64 (most accepting). The sub-scores were combined into an overall acceptability score. Open-ended questions were qualitatively analyzed using deductive content analysis following the TFA, and categories within constructs of ethicality are presented.

Results
21 autistic people completed the survey, and their median (range) sub-scores were OT 40 (16 – 56), SLP 43.5 (28 – 57), ABA 19.5 (8 – 48), PT 39.5 (28 – 57). Qualitative categories within the TFA construct of ethicality included: 1) concerns about harms and negative experiences that can potentially occur because of therapies; 2) a lack of support for what respondents identified as behavioural techniques or methods.

Conclusion
Findings are similar to existing perspectives that some autistic people have towards ABA-based therapies. However, these findings highlight that some autistic people may view any therapy as unacceptable if it incorporates behavioural methods. Further work is needed to better understand aspects of behavioral methods that are viewed as unacceptable, given that some strategies, such as positive reinforcement, are used in different therapies and with the general population. Future work should explore perspectives from larger samples.

Potential competing interests
Melanie Penner has received consulting fees from the province of Nova Scotia and Addis & Associates / Roche, and research grant funding from Autism Speaks.