bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of the impact of the inverse rotating scale on the body composition of shift workers
Authors: Cavalcante Gilberto , Silva Andressa , Silva Flavia , Crispim Cibele , Brant Valdênio , Guerreiro Renato , de Mello Marco 
Published: April 2023

Abstract
Change from the daily routine to night work and shift work leads to disturbances in the biological rhythms, which only slowly adapt to the new conditions. The processes, ingestion and metabolism and absorption of nutrients may present themselves in an opposite way in night shift workers, as a consequence of nighttime sleep deprivation causing misalignment of the circadian rhythm and dysregulation of nutrient intake and metabolism[1]. While the deleterious effects of inversion of the circadian rhythm caused by night work seem clear, rotating work schedules can favor physiological balance by reducing the loss of night sleep by diluting the days of night work among all workers on the scale and not to a specific group.
Purpose: We describe here the sleep profile, body composition, and basal metabolism in reverse rotating shift workers of operators of a mining company, in order to investigate whether shift work negatively impacts these conditions.
Methods: We aimed to assess whether the 4x1 scale model is harmful to the worker.
Results: sleep and body composition parameters did not be affect by shift work, not corroborating our hypothesis, that this scale would harm the health of the worker. It was impossible to observe differences in the body composition of shift workers considering only the duration of sleep.
Conclusion: Inverse rotating scale model evaluated in this study was interesting for shift workers, since both sleep time and the number of years worked in the shift were not associated with body composition as well as resting metabolism rate.
Genotypic and phenotypic diversity of the multidrug-resistant Mycobacterium tuberculosis strains from eastern India
Authors: Ghosh Arup , Biswas Viplov Kumar , Bal Himadri , Das Dasarathi , Pati Sanghmitra , Gupta Bhawna , Raghav Sunil Kumar 
Published: April 2023

Abstract

Background The genomic diversity of M. tuberculosis has been explored in the northern and southern states of India using whole genome sequencing, but insight into the eastern region remains limited. The study aims to understand the diversity and drug resistance phenotypes in eastern region using sequencing based approach.Methods A total of 118 M. tuberculosis isolates from Sikkim (n = 53), Meghalaya (n = 1), and Odisha (n = 48) were sequenced. After filtering out low quality isolates based on taxonomic classification and depth of coverage, variant calling was performed.Results We observed a high prevalence of multi-drug resistant (MDR) TB lineage 2 (n = 52) isolates in northeast whereas there was a mixed representation of lineage 1 (n = 30) & lineage 3 (n = 11) in Odisha. The MDR TB isolates from Sikkim posed a high rate of fluoroquinolone resistance and pairwise SNV distances(≤10) showed evidence of local transmission in the region. We observed occurrence of genetic variations in genes associated with bedaquiline and delamanid resistance.Conclusion Our findings show the diversity of M. tuberculosis vary across the eastern regions, in north eastern states lineage 2 has a dominant presence were as lineage 1 and 3 has mixed representation in Odisha. The high prevalence of fluoroquinolone resistance in north eastern region associated with variations in gyrA gene and local transmission.
Evaluation of methods for leaching heavy metals regarding environmental and economic factors
Authors: Król Anna , Jurczyk-Bunkowska Magdalena , Mizerna Kamila 
Published: April 2023

Abstract
The paper presents a specific model used for the assessment and selection of the method of heavy metals leaching from waste materials. A recommendation for the rational selection of leachability method was made, taking into account the crucial parameters affecting the leaching process derived from the authors' research experience. The assessment models were supplemented with characteristics relating to the cost and time required to perform the tests. An approach was developed that makes the weights of the assessment criteria dependent on the degree of environmental risk from particular heavy metals and waste types. Using multiple-criteria decision analysis four models were developed for the assessment and selection of a test method for the leaching of heavy metals from waste, differing in the way in which the weights of the assessment criteria depend on the potential threat the waste poses to the environment. A key element of the proposed approach is assigning the weights of the criterion to the toxicity of the heavy metal and the kind of wastes and their management scenario. The results obtained indicate that the current practice of only imposing a batch leaching test is inappropriate. The choice of method should be justified in terms of environmental safety and reasonable in terms of time and costs. The results of the paper indicate the possibility of using the proposed model in practice as a recommendation for the method for heavy metals leaching from waste materials and their further treatment in accordance with the principles of sustainable waste management.
WITHDRAWN: Lithological Variations over the Last Two Millennia within the Sedimentary Fillings of El- Guettiate in South East Tunisia: The Holocene Cyclostratigraphy
Authors: Bouabid Najia , Sra Feyda , Dutton Jarred , Tagorti Mohamed Ali 
Published: April 2023

Abstract
The Holocene sedimentary record of the Sebkha El Guettiate is located on the southern coast of Skhira, Gulf of Gabès in Tunisia. The cyclostratigraphic study depends on the idea that important segments of the stratigraphic record have recognizably encoded the orbital armature, periodic variations of the terrestrial insolation (Milankovitch cycles). It is a specific method of stratigraphy which studies the succession of different geological strata based on the results of climatology, astronomy and mathematical techniques. Holocene cyclostratigraphy is related to solar activity and other oceanographic and astronomical factors. This study aimed to test the climatic cyclicity along a core of Sebkha El-Guettiate located along the Mediterranean coast in Tunisia, by focusing on the study of the particle size distribution, in particular the variability of the sandy and silty fractions. Based on the dating of Zaibi et al. (2011) they were estimated to cover the last millennia with a sedimentation rate of 0.32 mm/year. This rate accepts the preparation of a depth-age model to perform the spectral analysis.Our analysis found cycles of ≈ 2000, ≈ 1600, 1300, 1000, 800, 595, 324 and 166 years. The 2000 year and 1000 year cycles are related to solar activity. The 1600 years cycle is likely related to internal forcing of the Atlantic Ocean. The 1300-year and 800-year cycles are probably related to the thermohaline circulation of the oceans. However, the other cycles of 600 years 324 years and 166 years are probably related to other oceanographic and atmospheric factors.
Diagnostic efficacy of Aspergillus galactomannan lateral flow assay in patients with hematological malignancies: a prospective multicenter study
Authors: Alhan Ozlem , Saba Rabin , Akalin Emin Halis , Ener Beyza , Yuce Zeynep Ture , Deveci Burak , Guncu Mehmet Mucahit , Kahveci Huseyin Nadir , Yilmaz Asu Fergun , Odabasi Zekaver 
Published: April 2023

Abstract
Background: A rapid and reliable diagnostic test is needed to reduce mortality through early diagnosis of invasive aspergillosis (IA) in patients with hematological malignancies.
Objective: To evaluate the efficacy of serum and bronchoalveolar lavage (BAL) Aspergillus galactomannan lateral flow assay (GM-LFA) in IA diagnosis and determine the correlation of GM-LFA with GM enzyme immunoassay (GM-EIA) in patients with hematological malignancies.
Methods: In this prospective multicenter study, we used serum and BAL fluid samples from patients with hematological malignancies and suspected IA and performed GM-LFA and GM-EIA. According to the EORTC/MSGERC criteria, patients were grouped as proven (n=6), probable (n=22), possible IA (n=55), or no IA (n=88). The performance of serum GM-LFA at 0.5 optical density index (ODI) and area under the curve (AUC) were calculated. Spearman’s correlation analysis and kappa statistics were performed to determine the agreement between the tests.
Results: GM-LFA showed an AUC of 0.832 in proven/probable IA (sensitivity [SEN], specificity [SPE], negative predictive value [NPV], and diagnostic accuracy were 75%, 100%, 92.6%, and 93.9%, respectively, at a 0.5 ODI) versus that in no IA. A moderate positive correlation was noted between the GM-LFA and GM-EIA scores (p=0.01). The observed agreement between the tests at 0.5 ODI was almost perfect (p<0.001). After excluding patients who received mold-active antifungal prophylaxis or treatment, the SEN, SPE, NPV, and diagnostic accuracy for proven/probable IA were 76.2%, 100%, 93.3%, and 94.5%, respectively.
Conclusions: Serum GM-LFA demonstrated high discriminatory power and good diagnostic performance for IA in patients with hematological malignancies.
Boosting-based machine learning approaches for diabetes prediction using Indian demographic and health survey-2021 data
Authors: Thotad Puneeth N , Bharamagoudar Geeta R. , Kallur Shanta S 
Published: April 2023

Abstract
This study examines the effectiveness of boosting-based machine learning classifiers in identifying diabetes in the Indian population. Traditional diagnostic methods for diabetes are time-consuming and prone to errors, and computer-aided diagnostic techniques can improve performance and reduce costs. The study analyzes data from the Indian Demographic and Health Survey 2021, focusing on women aged 19–49 who tested positive for diabetes. The dataset includes clinical, anthropometric, and biochemical components, and 12,103 positive responders was considered. The study adopts predictive exploration-based boosting machine learning models, including adaptive boosting, categorical boosting, extreme gradient boosting, gradient boosting, and light gradient boosting models. Feature extraction is performed using kernel principal component analysis. The extreme gradient boosting model performs well on the dataset, with accuracy, f1-score, precision, and recall values of 81% and 83%, 81% and 82%, 81% and 88%, and 81% and 76%, respectively, before and after applying kernel principal component analysis. Adaptive boosting performs poorly, while categorical, gradient, and light gradient boosting models perform moderately. The study achieves maximum accuracy, precision, the area under the curve, and recall.
Misophonia in Children and Adolescents: Age Differences, Risk Factors, Psychiatric and Psychological Correlates. A Pilot Study With Mothers’ Involvement
Authors: Siepsiak Marta , Turek Anna , Michałowska Magdalena , Gambin Małgorzata , Dragan Wojciesz 
Published: April 2023

Abstract
Misophonia is a debilitating disorder characterized by decreased sound tolerance. While it typically begins in childhood, research on its characteristics in this population is limited. We assessed 90 children aged 7–18 with and without misophonia, along with their mothers, using interviews, questionnaires, and performance-based tests. Younger children with misophonia were more likely to use aggression in response to triggers than older, while adolescents largely reported self-harm during triggers. Children with misophonia did not differ from their peers in terms of ADHD, ODD, ASD, dyslexia, social and emotional competencies, head injuries, epilepsy, tinnitus, being prematurely born, or delivered via cesarean sections. However, they had significantly higher symptoms of anxiety and depression, more frequent occurrences of OCD, migraines, and psychosomatic complaints. Their mothers self-reported postpartum depression significantly more frequently than mothers in the control group. There is a need for further research on pediatric misophonia, with the involvement and assessment of parents.
Age-related change in cortical thickness in adolescents at clinical-high risk for psychosis: a longitudinal study
Authors: Fortea Adriana , Eijndhoven Philip van , Calvet-Mirabent Angels , Ilzarbe Daniel , Batalla Albert , Serna Elena de la , Puig Olga , Castro-Fornieles Josefina , Dolz Montserrat , Tor Jordina , Parrilla Sara , Via Esther , Stephan-Otto Christian , Baeza Inmaculada , Sugranyes Gisela 
Published: April 2023

Abstract
Progression to psychosis has been associated with increased cortical thinning in the frontal, temporal and parietal lobes in individuals at clinical high-risk for the disease (CHR-P). The timing and spatial extent of these changes are thought to be influenced by age. However, most evidence so far stems from adult samples. Longitudinal studies are essential to understanding the neuroanatomical changes associated to transition to psychosis during adolescence, and their relationship with age. We conducted a longitudinal, multicentre study including adolescents at CHR-P and healthy controls (HC), aged 10–17 years. Structural images were acquired at baseline and at 18-month follow-up. Images were processed with the longitudinal pipeline in FreeSurfer. We used a longitudinal two-stage model to compute the regional cortical thickness (CT) change, and analyse between-group differences controlling for age, sex and scan, and corrected for multiple comparisons. Linear regression was used to study the effect of age at baseline. A total of 103 individuals (49 CHR-P and 54 HC) were included in the analysis. During follow-up, the 13 CHR-P participants who transitioned to psychosis exhibited greater CT decrease over time in the right parietal cortex compared to those who did not transition to psychosis and to HC. Age at baseline correlated with longitudinal changes in CT, with younger individuals showing greater cortical thinning in this region. The emergence of psychosis during early adolescence may have an impact on typical neuromaturational processes. This study provides new insights on the cortical changes taking place prior to illness onset.
Antibacterial peptide PMAP-37(F34-R), expressed in Pichia pastoris, is effective against pathogenic bacteria and preserves plums
Authors: Dong Chunming , Xu Lijun , Lu Weitao , Li Mengru , Zhang Rui , Liu Jian , Sun Yanyan , Chu Xinlei 
Published: April 2023

Abstract
Background Recently, researchers have focused on the search for alternatives to conventional antibiotics. Antimicrobial peptides are small bioactive peptides that regulate immune activation and have antibacterial activity with a reduced risk of bacterial resistance. Porcine myeloid antibacterial peptide 37 (PMAP-37) is a small-molecule peptide with broad-spectrum antibacterial activity isolated from pig bone marrow, and PMAP-37(F34-R) is its analogue. In this study, PMAP-37(F34-R) was recombinantly expressed in Pichia pastoris, and the recombinant peptide was further investigated for its antibacterial properties, mechanism and preservative in plums.Results To obtain a Pichia pastoris strain expressing PMAP-37(F34-R), we constructed a plasmid expressing recombinant PMAP-37(F34-R) (pPICZα-PMAP-37(F34-R)-A) and introduced it into Pichia pastoris. Finally, we obtained a highly active recombinant peptide, PMAP-37(F34-R), which inhibited the activity of both Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration is 0.12–0.24 µg/mL, and it can destroy the integrity of the cell membrane, leading to cell lysis. It has good stability and is not easily affected by the external environment. Moreover, hemolysis experiments showed that PMAP-37(F34-R) had a low hemolytic ability against mammalian cells. Additionally, PMAP-37(F34-R) showed a good preservative effect in plums. The decay and weight loss rates of the treated samples were significantly lower than those of the control group, and the respiratory intensity of the fruit was delayed in the experimental group.Conclusions In this study, we constructed a recombinant Pichia pastoris strain, which is a promising candidate for extending the shelf life of fruits and has potential applications in the development of new preservatives.
Being a Child with ADHD, and Parent of them during the Pandemic Period
Authors: Çimen İrem Damla , Erark Müjdat , Gündoğdu Özlem Yıldız , Annaç Nefise , Özboduroğlu Akın Altuğ 
Published: April 2023

AbstractAlthough genetics is an important factor in the etiology of attention-deficit/hyperactivity disorder (ADHD), psychosocial factors such as parental attitudes, changes in environment and order are also known to be important in determining severity and prognosis. During the pandemic and lockdown periods, the daily routines and habits of families and children changed significantly. It was aimed to evaluate the mental status and parent-child relationship of parents with children diagnosed with ADHD during the lockdown in Turkey. It also aimed to examine the relationship between these variables and the change in ADHD symptom severity in children. The study included 92 children/adolescents aged 6–18 years who were referred to the child psychiatry department. Parents were given a socio-demographic form, the Turgay scale, the parent-child relationship scale and the Brief Symptom Inventory (BSI). There was a significant increase in the severity of ADHD dominant type and conduct disorder (CD) symptom severity in the group who spent their free time on the Internet, the increase in symptom severity was significant with the change in caregivers, and in children whose parents left their jobs. A significant positive correlation was found between all subscales of the BSI and ADHD subtypes, oppositional defiant disorder (ODD) and CD symptom severity. A positive correlation was found between a negative parent-child relationship and ADHD subtypes, ODD and CD symptom severity. It is believed that the results of the study will be useful in approaching children diagnosed with ADHD in case the pandemic intensifies again or restrictions are required for various reasons.
Molecular epidemiology of Enterocytozoon bieneusi from Foxes and Raccoon dogs in the Henan and Hebei Provinces
Authors: Chen Minghui , Wang Haidong , Li Xinmiao , Guo Yunan , Lu Ying , Zheng Liping , Liang Guoqing , Sui Yuzhen , Wang Bukang , Dai Hongyu , Dong Haiju , Zhang Longxian 
Published: April 2023

Abstract
Background Enterocytozoon bieneusi is a zoonotic pathogen widely distributed in animals and humans. It can cause diarrhea and even death in immunocompromised hosts. Approximately 600 internal transcribed spacer (ITS) genotypes have been identified in E. bieneusi. E. bieneusi in foxes and raccoon dogs are known to have zoonotic potential. However, there are only a few studies about E. bieneusi genotype identification and epidemiological survey in foxes and raccoon dogs in Henan and Hebei province. Thus, the present study investigated the infection rates and genotypes of E. bieneusi in farmed foxes and raccoon dogs in the Henan and Hebei provinces.Result A total of 704 and 884 fecal specimens were collected from foxes and raccoon dogs, respectively. Nested PCR was conducted based on ITS of ribosomal RNA (rRNA), and then multilocus sequence typing (MLST) was conducted to analyze the genotypes. The result showed that infection rates of E. bieneusi in foxes and raccoon dogs were 18.32% and 5.54%, respectively. Ten E. bieneusi genotypes with zoonotic potential ( NCF2, NCF3, D, EbpC, CHN-DC1, SCF2, CHN-F1, Type IV, BEB4, and BEB6) were identified in foxes and raccoon dogs. Totally 178 ITS-positive DNA specimens were identified from foxes and raccoon dogs and these specimens were then subjected to MLST analysis. In the MLST analysis, 12, 2, 7 and 8 genotypes were identified in at the mini-/ micro-satellite loci MS1, MS3, MS4 and MS7, respectively. A total of 14 multilocus genotypes were generated using ClustalX 2.1 software. Overall, the present study evaluated the infection of E. bieneusi in foxes and raccoon dogs in Henan and Hebei province, and investigated the zoonotic potential of the E. bieneusi in foxes and raccoon dogs.Conclusions These findings expand the geographic distribution information of E. bieneusi’ host in China and was helpful in preventing against the infection of E. bieneusi with zoonotic potential in foxes and raccoon dogs.
Network Coding Construction for a Special Class of Three Unicast Sessions
Authors: Baoxing PU 
Published: April 2023

Abstract
For a special class of three unicast sessions, in which the maximum flow from each sender to each receiver is the same positive integer k, a network coding approach is proposed. A multigeneration mixed strategy, in which (2×n + 1) consecutive generations are taken as a mixed set, is adopted. The precoding strategy is adopted at the senders, random linear network coding technology is adopted at intermediate nodes to transmit data, and interference alignment technology is used at the receivers to eliminate some interference information. The proposed approach can construct a network coding data transmission scheme, in which the transmission rate vector of the senders is [(n + 1)×k/(2×n + 1), n×k/(2×n + 1), n×k/(2×n + 1)]. The feasibility of the proposed approach is mathematically deduced and proven, and the simulation results verify the conclusions of the theoretical analysis.
Effects of Nitrogen Starvation on Growth and Biochemical Composition of Some Microalgae Species
Authors: ŞİRİN Pınar AKDOĞAN , SERDAR Serpil 
Published: April 2023

AbstractNitrogen is one of the most important nutrient sources for the growth of microalgae. We studied the effects of nitrogen starvation on the growth responses, biochemical composition and fatty acid profile ofDunaliella tertiolecta, Phaeodactylum tricornutumandNannochloropsis oculata. The lack of nitrogen caused changes in carbohydrate, protein, lipid and fatty acid composition in all examined microalgae. The carbohydrate content increased 59% inD. tertiolecta, while the lipid level increased 139% inP. tricornutumunder nitrogen stress conditions. Nitrogen starvation increased the oligosaccharide and polysaccharide contents ofD. tertiolecta4.18-fold and 3.77-fold, respectively. Furthermore, triacylglycerol (TAG) levels inN. oculataandP. tricornutumincreased 2.3-fold and 7.4-fold, respectively. The dramatic increase in the amount of TAG is important for the use of these microalgae as raw materials in biodiesel. Nitrogen starvation increased the amounts of oligosaccharides and polysaccharides ofD. tertiolecta, while increased eicosapentaenoic acid (EPA) inN. oculataand docosahexaenoic acid (DHA) content inP. tricornutum. The amount of polyunsaturated fatty acids (PUFAs), EPA, DHA, oligosaccharides and polysaccharides in microalgal species can be increased without using the too costly nitrogen source in the culture conditions, which can reduce the most costly of living feeding.
Study on Measurement Method of Three-dimensional Position of Unlabeled Microspheres under Bright Background
Authors: Xu Ke , Wang Bingge 
Published: April 2023

Abstract
Using computer vision technology to obtain the position and trajectory data of particle probe microspheres from microscope images has important significance and value in the molecular field. However, most of the existing microsphere measurement methods are based on transmission, which can only be measured under transparent samples and substrates, are not suitable for the application scenario of living cell measurement. In this paper, a method based on reflectivity imaging is proposed to measure the three-dimensional position of the dark microspheres in the bright field. Based on the outermost ring radius method, the relationship between the inner ring radius of the microsphere spot and the out-of-focus distance was explored to measure the coordinates in the Z direction. Cardiomyocytes were combined with 10um size silica microspheres. Experiments show that in a bright field with high perturbation environment, it can achieve high precision measurement of dark microspheres and achieve three-dimensional position measurement with accuracy of 50nm in XY direction and 100nm in Z direction.