bluuebunny/crossref_metadata_2025_split huggingface dataset:
Which is more deleterious to cognitive performance? Interictal epileptiform discharges vs anti‐seizure medication
Authors: Warsi Nebras M. , Wong Simeon M. , Gorodetsky Carolina , Suresh Hrishikesh , Arski Olivia N. , Ebden Mark , Kerr Elizabeth N. , Smith Mary Lou , Yau Ivanna , Ochi Ayako , Otsubo Hiroshi , Sharma Rohit , Jain Puneet , Weiss Shelly , Donner Elizabeth J. , Snead O. Carter , Ibrahim George M. 
Published: February 2023

AbstractChildren with epilepsy commonly have comorbid neurocognitive impairments that severely affect their psychosocial well‐being, education, and future career prospects. Although the provenance of these deficits is multifactorial, the effects of interictal epileptiform discharges (IEDs) and anti‐seizure medications (ASMs) are thought to be particularly severe. Although certain ASMs can be leveraged to inhibit IED occurrence, it remains unclear whether epileptiform discharges or the medications themselves are most deleterious to cognition. To examine this question, 25 children undergoing invasive monitoring for refractory focal epilepsy performed one or more sessions of a cognitive flexibility task. Electrophysiological data were recorded to detect IEDs. Between repeated sessions, prescribed ASMs were either continued or titrated to <50% of the baseline dose. Hierarchical mixed‐effects modeling assessed the relationship between task reaction time (RT), IED occurrence, ASM type, and dose while controlling for seizure frequency. Both presence (β ± SE = 49.91 ± 16.55 ms, p = .003) and number of IEDs (β ± SE = 49.84 ± 12.51 ms, p < .001) were associated with slowed task RT. Higher dose oxcarbazepine significantly reduced IED frequency (p = .009) and improved task performance (β ± SE = −107.43 ± 39.54 ms, p = .007). These results emphasize the neurocognitive consequences of IEDs independent of seizure effects. Furthermore, we demonstrate that inhibition of IEDs following treatment with select ASMs is associated with improved neurocognitive function.
Effective population size in a partially clonal plant is not predicted by the number of genetic individuals
Authors: Gargiulo Roberta , Waples Robin S. , Grow Adri K. , Shefferson Richard P. , Viruel Juan , Fay Michael F. , Kull Tiiu 
Published: February 2023

AbstractEstimating effective population size (Ne) is important for theoretical and practical applications in evolutionary biology and conservation. Nevertheless, estimates of Ne in organisms with complex life‐history traits remain scarce because of the challenges associated with estimation methods. Partially clonal plants capable of both vegetative (clonal) growth and sexual reproduction are a common group of organisms for which the discrepancy between the apparent number of individuals (ramets) and the number of genetic individuals (genets) can be striking, and it is unclear how this discrepancy relates to Ne. In this study, we analysed two populations of the orchid Cypripedium calceolus to understand how the rate of clonal versus sexual reproduction affected Ne. We genotyped >1000 ramets at microsatellite and SNP loci, and estimated contemporary Ne with the linkage disequilibrium method, starting from the theoretical expectation that variance in reproductive success among individuals caused by clonal reproduction and by constraints on sexual reproduction would lower Ne. We considered factors potentially affecting our estimates, including different marker types and sampling strategies, and the influence of pseudoreplication in genomic data sets on Ne confidence intervals. The magnitude of Ne/Nramets and Ne/Ngenets ratios we provide may be used as reference points for other species with similar life‐history traits. Our findings demonstrate that Ne in partially clonal plants cannot be predicted based on the number of genets generated by sexual reproduction, because demographic changes over time can strongly influence Ne. This is especially relevant in species of conservation concern in which population declines may not be detected by only ascertaining the number of genets.
Where land meets sea: Intertidal areas as key‐habitats for sharks and rays
Authors: Leurs Guido , Nieuwenhuis Brian O. , Zuidewind Thije J. , Hijner Nadia , Olff Han , Govers Laura L. 
Published: February 2023

AbstractIntertidal habitats (i.e. marine habitats that are (partially) exposed during low tide) have traditionally been studied from a shorebird‐centred perspective. We show that these habitats are accessible and important to marine predators such as elasmobranchs (i.e. sharks and rays). Our synthesis shows that at least 43 shark and 45 ray species, of which 54.5% are currently threatened, use intertidal habitats. Elasmobranchs use intertidal habitats mostly for feeding and as refugia, but also for parturition and thermoregulation. However, the motivation of intertidal habitat use remains unclear due to limitations to observe elasmobranch behaviour in these dynamic habitats. We argue that elasmobranch predators can play an important role in intertidal food webs by feeding on shared resources during high tide (i.e. ‘high‐tide predators’), which are accessible and also consumed by terrestrial or avian predators during low tide (i.e. ‘low‐tide predators’). In addition, elasmobranchs are able to change the bio‐geomorphology of intertidal habitats by increasing habitat heterogeneity due to feeding activities and may also alter resource availability for other consumers. We discuss how the ecological role of elasmobranchs in intertidal habitats is being affected by the continued overexploitation of these species, and conversely, how the global loss of intertidal areas poses an additional threat to an already vulnerable taxonomic group. We conclude that studies on intertidal ecology should include both low‐tide (e.g. shorebirds) and high‐tide (e.g. elasmobranchs) predatory guilds and their ecological interactions. The global loss of elasmobranch predatory species and intertidal habitat provides additional compelling arguments for the conservation of these areas.
Enzymology of reactive intermediate protection: kinetic analysis and temperature dependence of the mesophilic membrane protein catalyst MGST1
Authors: Cebula Marcus , Morgenstern Ralf 
Published: February 2023

Glutathione transferases (GSTs) are a class of phase II detoxifying enzymes catalysing the conjugation of glutathione (GSH) to endogenous and exogenous electrophilic molecules, with microsomal glutathione transferase 1 (MGST1) being one of its key members. MGST1 forms a homotrimer displaying third‐of‐the‐sites‐reactivity and up to 30‐fold activation through modification of its Cys‐49 residue. It has been shown that the steady‐state behaviour of the enzyme at 5 °C can be accounted for by its pre‐steady‐state behaviour if the presence of a natively activated subpopulation (~ 10%) is assumed. Low temperature was used as the ligand‐free enzyme is unstable at higher temperatures. Here, we overcame enzyme lability through stop‐flow limited turnover analysis, whereby kinetic parameters at 30 °C were obtained. The acquired data are more physiologically relevant and enable confirmation of the previously established enzyme mechanism (at 5 °C), yielding parameters relevant for in vivo modelling. Interestingly, the kinetic parameter defining toxicant metabolism, kcat/KM, is strongly dependent on substrate reactivity (Hammett value 4.2), underscoring that glutathione transferases function as efficient and responsive interception catalysts. The temperature behaviour of the enzyme was also analysed. Both the KM and KD values decreased with increasing temperature, while the chemical step k3 displayed modest temperature dependence (Q10: 1.1–1.2), mirrored in that of the nonenzymatic reaction (Q10: 1.1–1.7). Unusually high Q10 values for GSH thiolate anion formation (k2: 3.9), kcat (2.7–5.6) and kcat/KM (3.4–5.9) support that large structural transitions govern GSH binding and deprotonation, which limits steady‐state catalysis.
Dynamic changes and correlation analysis of protease activities, texture and flavour compounds during fish fermentation (suanyu) using mixed culture (Enterococcus rivorum and Enterococcus lactis)
Authors: Yuan Li , Zhang Qianqian , Zhao Feng , Jin Wengang , Shi Tong , Xiong Zhiyu , Gao Ruichang 
Published: February 2023

SummaryThis research investigated the dynamic changes and correlations of protease activity, material texture and flavour compounds during the fermentation (13 days) of crucian (Carassius auratus). Mixed starter fermentation (Enterococcus rivorum and Enterococcus lactis) and natural fermentation were used for the study. Results showed that the protease activity, TCA‐soluble protein and flavour substances were increased with fermentation time and were higher in the mixed fermentation group. The results of relative odour activity value showed that some aldehydes played a major role in the flavour of the product, such as octanal, nonanal and (E)‐2‐nonenal. The correlation analysis showed that protease activity and material texture (hardness and chewiness) had a significantly positive correlation with many flavour compounds (octanal, nonanal, glutamic acid, etc.). This work will provide a theoretical reference for the application of mixed starter cultures and for improving the quality of suanyu by increasing protease activity and changing the texture of the material.
Development of synbiotic vegan beverages: probiotic viability, sensory profile, consumers' acceptance and functional stability
Authors: Valero‐Cases Estefanía , Frutos María José , Pérez‐Llamas Francisca 
Published: February 2023

SummaryThe development of synbiotic vegan drinks expands the probiotic food options on the market as an alternative to dairy products and with no added sugars. This study aimed to investigate the impact of fermentation and inulin addition in mixed carrot‐orange juice with Lactiplantibacillus plantarum (LP) on the sensory profile, consumer acceptance and functional stability. The results demonstrated that all fermented juices had high antioxidant capacity (AOC) and were a rich source of bioactive compounds such as β‐carotene and phenolics. However, only the β‐carotene showed a high correlation with the AOC. Inulin could improve LP survival, remaining at high concentration during the 21 days of storage. Only fermented juices with 2% inulin showed a positive correlation with sweet taste and had the best impact on sensorial properties. Fermentation with LP and inulin addition could contribute to the improvement of orange flavour and taste. Penalty analysis showed that the control‐unfermented juices were the samples with a clear need for improvement with respect to the fermented ones. Therefore, carrot‐orange juices represent a promising vegan food matrix for probiotic delivery.
Effects of pomelo peel sponge layer insoluble dietary fibre addition on the properties of the dough and bread
Authors: Sang Jiaqi , Wen Jing , Li Lu , Xu Yujuan , Gu Qingqing , Yu Yuanshan , Peng Jian 
Published: February 2023

SummaryIn this study, the influences of different proportions (0%, 5%, 10%, 15% and 20% w/w) of pomelo peel sponge layer insoluble dietary fibre (PP‐IDF) on the technological quality, structural character and sensory evaluation of wheat dough and bread were studied. Results showed that the addition of PP‐IDF improved the viscoelasticity of dough and reduced its enthalpy of freezing–thawing. Meanwhile, PP‐IDF significantly improved the quality of bread during storage. Compared with the control, the 7‐day moisture rate of bread containing PP‐IDF increased by 3.50–6.80%, indicating that PP‐IDF retarded the staling process of bread. Moreover, SEM images showed that the bread prepared using PP‐IDF had a more heterogeneous crumb microstructure, and the higher the addition level of PP‐IDF, the larger the void structure. The results of the texture profile analysis indicated that the hardness and chewiness of bread increased with the incorporation of PP‐IDF, suggesting that the addition of PP‐IDF had a negative effect on the texture property of bread. Furthermore, the sensory evaluation of bread was performed, and the taste of the bread was better when PP‐IDF addition content was 5–10%. The results of this study revealed that PP‐IDF had great potential for the development of innovative bread recipes.
Factors contributing to nurse resignation during COVID‐19: A qualitative descriptive study
Authors: Jarden Rebecca J. , Scott Suzanne , Rickard Nonie , Long Karrie , Burke Shirley , Morrison Morag , Mills Leanne , Barker Emma , Sharma Karan , Twomey Bernadette 
Published: February 2023

AbstractAimsTo explore and describe registered nurses' perceptions and experiences of work well‐being extending from what inspired them to join the healthcare organization, what created a great day at work for them, through to what may have supported them to stay.DesignQualitative descriptive study.MethodsThirty‐nine Australian nurses who resigned in 2021 from two metropolitan healthcare organizations in Victoria were interviewed in 2022, each for 30–60 min. The semi‐structured interview transcripts were transcribed verbatim and analysed inductively and thematically.ResultsFour themes were constructed for each of the key research questions. Inspiration to join the organizations transpired through organizational reputation, recruitment experiences, right position and right time, fit and feel. A great day at work was created through relationships with colleagues, experiences with managers, adequate resourcing and delivering quality care. Factors contributing to nurses resigning included COVID‐19, uncertainty of role, workload and rostering, and finally, not feeling supported, respected and valued. Factors that may have supported the nurses to stay included flexible work patterns and opportunities, improved workplace relationships, workload management and support, and supportive systems and environments. Cutting across these themes were five threads: (1) relationships, (2) communication, (3) a desire to learn and develop, (4) work–life balance and (5) providing quality patient care.ConclusionsNovel ways of working and supporting individuals, teams and organizations are needed to maintain and sustain nurses. The nurses' inspiration, what created a great day at work, and support needed to stay highlighted the importance of workplace initiatives to build nursing career pathways, provide equitable opportunities for professional development, workload and roster flexibility and implement professional relationship‐enhancing actions to foster authentic civility.Impact

This study contributes an in‐depth exploration of the perceptions and experiences of nurses who resigned from two healthcare organizations and provides a description of (1) what inspired these nurses to initially join the organization, (2) what they perceived created a great day at work for them, (3) the factors contributing to their resignation and (4) what may have supported them to stay.

The reasons nurses resign from an organization were identified as complex and multi‐factorial, with opportunities for promoting nursing career pathways, addressing equity in opportunities and implementing professional relationship‐enhancing actions.

These contributions add both context and opportunity to strengthen organizational initiatives to attract, sustain and retain nurses.
Embedding nurse‐led supportive care in an outpatient service for patients with Chronic Obstructive Pulmonary Disease
Authors: Ora Linda , Wilkes Lesley , Mannix Judy , Gregory Linda , Luck Lauretta 
Published: February 2023

AbstractAimTo describe a small multidisciplinary team's experience of the process of embedding nurse‐led supportive care into an existing Chronic Obstructive Pulmonary Disease outpatient service.DesignCase study methodologyMethodsData were collected from multiple sources including key documents and semi‐structured interviews with healthcare professionals (n = 6) conducted between June and July 2021. A purposive sampling strategy was used. Content analysis was applied to key documents. Interviews were transcribed verbatim and analysed using an inductive approach.ResultsSubcategories under the four‐stage process were identified from the data. Assessment: evidence of needs of patients with Chronic Obstructive Pulmonary Disease; gaps in care and evidence of other models of supportive care. Planning: setting the supportive care service structure and intention; resources and funding; leadership, specialization and respiratory/palliative care roles. Implementation: relationships and trust; embedding supportive care and communication. Evaluation: benefits and positive outcomes for staff and patients, and, improvements and future considerations for supportive care in the COPD service.ConclusionA collaboration between respiratory and palliative care services resulted in successfully embedding nurse‐led supportive care in a small outpatient service for patients with Chronic Obstructive Pulmonary Disease. Nurses are well placed to lead new models of care that aim to address unmet biopsychosocial‐spiritual needs of patients. More research is needed to evaluate nurse‐led supportive care in Chronic Obstructive Pulmonary Disease and other chronic illness settings; the effectiveness of nurse‐led supportive care from the perspective of patients and caregivers and the impact of nurse‐led supportive care on health service usage.Patient or Public ContributionThe development of the model of care is informed by ongoing discussions with patients with COPD and their caregivers.Data availability statement: Research data are not shared (due to ethical restrictions).ImpactEmbedding nurse‐led supportive care in an existing Chronic Obstructive Pulmonary Disease outpatient service is achievable.Nurses with clinical expertise can lead innovative models of care that address the unmet biopsychosocial‐spiritual needs of patients with conditions such as Chronic Obstructive Pulmonary Disease.Nurse‐led supportive care may have utility and relevance in other chronic disease contexts.
Proteomic profiling of epicardial fat in heart failure with preserved versus reduced and mildly reduced ejection fraction
Authors: Gao Qian , He Shan , Peng Yuanshu , Su Pixiong , Zhao Lei 
Published: February 2023

AbstractIn order to explore the proteomic signatures of epicardial adipose tissue (EAT) related to the mechanism of heart failure with reduced and mildly reduced ejection fraction (HFrEF/HFmrEF) and heart failure (HF) with preserved ejection fraction (HFpEF), a comprehensive proteomic analysis of EAT was made in HFrEF/HFmrEF (n = 5) and HFpEF (n = 5) patients with liquid chromatography–tandem mass spectrometry experiments. The selected differential proteins were verified between HFrEF/HFmrEF (n = 20) and HFpEF (n = 40) by ELISA (enzyme‐linked immunosorbent assay). A total of 599 EAT proteins were significantly different in expression between HFrEF/HFmrEF and HFpEF. Among the 599 proteins, 58 proteins increased in HFrEF/HFmrEF compared to HFpEF, whereas 541 proteins decreased in HFrEF/HFmrEF. Of these proteins, TGM2 in EAT was down‐regulated in HFrEF/HFmrEF patients and was confirmed to decrease in circulating plasma of the HFrEF/HFmrEF group (p = 0.019). Multivariate logistic regression analysis confirmed plasma TGM2 could be an independent predictor of HFrEF/HFmrEF (p = 0.033). Receiver operating curve analysis indicated that the combination of TGM2 and Gensini score improved the diagnostic value of HFrEF/HFmrEF (p = 0.002). In summary, for the first time, we described the proteome in EAT in both HFpEF and HFrEF/HFmrEF and identified a comprehensive dimension of potential targets for the mechanism behind the EF spectrum. Exploring the role of EAT may offer potential targets for preventive intervention of HF.
Design consideration and optimization of process parameters in fiber extraction unit via modelling studies
Authors: Vishnu V. , Harikrishnan M. P. , Warrier Aswin S. , Mahanti Naveen Kumar , Basil M. , Venkatesh T. , Pandiselvam R. , Kothakota Anjineyulu 
Published: February 2023

AbstractDemand for natural fibers as an alternative to synthetic fibers is on the rise. Constraints of current fiber extraction units are limited to single raw material and has low energy efficiency. Hence, this study was taken up for the design and development of a multiple fiber extractor, modelling and optimization of the machine operating parameters, and economic analysis of the developed prototype. The designed machine operates based on the raspador principle. Box Behnken design (BBD) and artificial neural network (ANN) were used to evaluate the performance and optimize the variables of the designed multiple fiber extractor. The input variables included roller speed (550, 735, and 920 rpm), gap/clearance of the roller (2, 3, and 4 mm) and the pitch/distance between the spline slots (0.5, 1.5, and 2.5 mm), while the output variables were yield (kg), capacity (kg/h), energy consumption (kJ), and efficiency (%).The prediction accuracy of quadratic models (R2 = 0.962–0.985) developed in BBD were better than the ANN models (R2 = 0.831–0.946). The optimized combination was found to be 714.23 rpm roller speed, 3.12 mm gap, and 1.49 mm pitch of the blade, which yielded (1.48 kg) a desirability value of 0.95. The XRD results indicated the highest crystallinity index was recorded for Pineapple leaf fiber compared to banana fiber. SEM results revealed that extracted BF having typical network structure (cellulose, hemicellulose, lignin, and waxes) compared to smooth PLF (lignin and hemicellulose). The fibers extracted using the developed machine was utilized for making biodegradable cutlery.Practical ApplicationsThis study was taken up to design a multifiber extractor, to extract fibers from agricultural residues after their useful parts are taken for consumption or processing. Thus, a single machine capable of handling multiple byproducts can utilize those residues, which were previously discarded as waste. Moreover, the extracted fibers are used for the manufacture of biodegradable cutlery. With a small investment, this machine can provide an additional income to the farmers, along with taking away the concern regarding waste disposal.
The effect of specimen preparation on the nanostructure of SLES aqueous solutions observed by cryo‐TEM
Authors: Lifshiz‐Simon Sapir , Talmon Yeshayahu 
Published: February 2023

AbstractSodium lauryl ether sulphate (SLES) is a detergent widely used in cosmetics and personal‐care industries; hence, it is of particular interest to study the self‐assembled nanostructure it forms at different conditions. Cryogenic transmission electron microscopy (cryo‐TEM) is the most suitable technique for the direct‐imaging of such systems. However, since SLES is sensitive to flow and shear, specimen preparation artefacts may misrepresent the native state of the solution. In this paper, we present different cryo‐TEM specimen preparation methods, and show how they affect the nanostructure of the system. In fact, for this system, we were able to record the native state of the solution only after sufficient time of on‐the‐grid relaxation (OGR) after blotting. Here, we also intend to point out the importance of considering the nature of the solution when preparing cryo‐TEM specimens.