bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robust tobacco smoking self-report in two cohorts: pregnant women or men and women living with or without HIV
Authors: Smith Marie-Soleil R. , Saberi Sara , Ajaykumar Abhinav , Zhu Mayanne M. T. , Gadawski Izabelle , Sattha Beheroze , Maan Evelyn J. , Van Shalkwyk Julie , Elwood Chelsea , Pick Neora , Murray Melanie C. M. , Boucoiran Isabelle , Money Deborah M. , Côté Hélène C. F. 
Published: May 2023

AbstractUnderstanding the true burden of tobacco smoking on adverse pregnancy outcomes is critical in generating appropriate interventions to improve outcomes. Self-reporting of human behaviour that is associated with stigma is associated with underreporting in general and may bias the impact of smoking in studies; however, self-reporting is frequently the most practical method of gleaning this information. The objective of this study was to evaluate concordance between self-reported smoking and concentrations of plasma cotinine, a biomarker of smoking, among participants enrolled in two related HIV cohorts. A total of 100 pregnant women (76 living with HIV [LWH] and 24 negative controls) in their third trimester, and 100 men and non-pregnant women (43 LWH and 57 negative controls) were included. Among all participants, 43 pregnant women (49% LWH and 25% negative controls) and 50 men and non-pregnant women (58% LWH and 44% negative controls) were self-reported smokers. The odds of discordance between self-reported smoking and cotinine levels were not significantly different between self-reported smokers and non-smokers, nor between pregnant women and others, but were significantly increased, regardless of self-reported status, among people LWH compared to negative controls. The overall concordance between plasma cotinine and self-reported data among all participants was 94% with a sensitivity and specificity of 90% and 96%, respectively. Taken together, these data demonstrate that participant surveying in a non-judgemental context can lead to accurate and robust self-report smoking data among both persons LWH and not, including in the context of pregnancy.
Prediction formulae of sagittal alignment in thoracolumbar kyphosis secondary to ankylosing spondylitis after osteotomy
Authors: Luo Jianzhou , Yang Zili , Duan Chunguang , Feng Xujiao , Tan Lei , Wei Yanzhe , Jiang Li , Wu Tailin 
Published: May 2023

AbstractTo construct and validate prediction formulae of sagittal alignment in thoracolumbar kyphosis secondary to ankylosing spondylitis (AS) after osteotomy. A total of 115 AS patients who suffered from thoracolumbar kyphosis and underwent osteotomy were enrolled, with 85 patients in derivation group and 30 patients in validation group. Radiographic parameters were measured on lateral radiographs, including thoracic kyphosis, lumbar lordosis (LL), T1 pelvic angel (TPA), sagittal vertical axis (SVA), osteotomized vertebral angle, pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), and PI and LL mismatch (PI-LL). Prediction formulae of SS, PT, TPA and SVA were established; and their effectiveness was evaluated. There was no significant difference in baseline characteristics between the two groups (p > 0.05). In derivation group, LL and PI-LL were correlated with SS, and were then used to establish the prediction formula of SS[SS = − 12.791–0.765 × (LL) + 0.357 × (PI-LL), R2 = 68.3%]; PI and PI-LL were correlated with PT, and the prediction formula of PT were thus established[PT = 12.108 + 0.402 × (PI-LL) + 0.252 × (PI), R2 = 56.8%]; PT, PI-LL and LL were correlated with TPA, and were used to establish the prediction formula of TPA[TPA = 0.225 + 0.597 × (PT) + 0.464 × (PI-LL)-0.161 × (LL), R2 = 87.4%]; PT, PI-LL and age were correlated with SVA, and were used to establish the prediction formula of SVA[SVA = 36.157 + 2.790 × (PI-LL) + 1.657 × (Age)-1.813 × (PT), R2 = 41.5%]. In validation group, the predictive SS, PT, TPA and SVA were basically consistent with corresponding real values; and the mean error between predictive values and real values was of 1.3° in SS, 1.2° in PT, 1.1° in TPA and 8.6 mm in SVA. Postoperative SS, PT, TPA and SVA could be predicted with PI and the planned LL and PI-LL using prediction formulae, providing a method for AS kyphosis to plan postoperative sagittal alignment. Change of pelvic posture after osteotomy was quantitatively evaluated using the formulae.
Atretic preovulatory follicles could be precursors of ovarian lutein cysts in the pig
Authors: Ziecik Adam J. , Likszo Pawel , Klos Jan , Gromadzka-Hliwa Katarzyna , Knapczyk-Stwora Katarzyna , Peltoniemi Olli , Gajewski Zdzislaw , Kaczmarek Monika M. 
Published: May 2023

AbstractOvarian cysts contribute to reduced reproductive performance in pigs. Unfortunately, the mechanism of lutein cysts formation remains unknown. Here, we compared the endocrine and molecular milieus of intact, healthy preovulatory follicles (PF), gonadotropin (eCG/hCG)-induced healthy and atretic-like PF, as well as gonadotropin-provoked and spontaneous ovarian cysts in gilts. Several endocrine and molecular indicators and microRNA were compared in walls of PF and cysts. Intact and healthy PF, showed high estradiol/androstendione and low progesterone levels associated with CYP17A1, HSD17B1, and CYP19A1 elevation and reduced StAR/HSD3B1 protein expression. In contrast, low estradiol/androstendione and high progesterone concentrations, accompanied by decreased CYP17A1, HSD17B1, CYP19A1 and increased HSD3B1 protein abundance, appeared in atretic-like PF, gonadotropin-induced and spontaneous cysts. High progesterone receptor (PGR) protein abundance was maintained in intact and healthy PF, while it dropped in atretic-like PF, gonadotropins-induced and spontaneous cysts. The atretic PF showed high level of TNFα compared to healthy PF. In conclusion, follicular lutein cysts could be recruited from atretic-like PF with lost estrogenic milieu and inability to ovulate. Ovulatory cascade was presumably disrupted by a low PGR and high TNFα levels associated with earlier luteinization of follicular walls. These results suggest a novel mechanism of lutein ovarian cysts development in pigs and, perhaps, other species.
Factors influencing axial elongation in myopic children using overnight orthokeratology
Authors: Huang Zhu , Zhao Wei , Mao Ying-zheng , Hu Shan , Du Chi-Xin 
Published: May 2023

AbstractSeveral factors influence axial length in children with myopia treated using overnight orthokeratology. To identify these factors, this retrospective study collected axial length and corneal aberration data on 78 eyes before and 1-year after orthokeratology. Patients were divided according to axial elongation (cut-off, 0.25 mm/year). Baseline characteristics included age, sex, spherical equivalent refraction, pupil diameter, axial length, and orthokeratology lens type. Corneal shape effects were compared through tangential difference maps. Group differences in higher-order aberrations of a 4 mm zone were compared at baseline and 1-year following therapy. Binary logistic regression analysis was conducted to identify the variables determined for axial elongation. Significant differences between both groups included the initial age of wearing orthokeratology lenses, type of orthokeratology lens, size of central flattening area, corneal total surface C12 (1-year), corneal total surface C8 (1-year), corneal total surface spherical aberration (SA) (1-year root mean square [RMS] values), change in total corneal surface C12, and change in front and total corneal surface SA (RMS values). The age when wearing an orthokeratology lens was the most important factor influencing axial length in children with orthokeratology-treated myopia, followed by lens type and change in the C12 of the total corneal surface.
Ceramide present in cholangiocarcinoma-derived extracellular vesicle induces a pro-inflammatory state in monocytes
Authors: Oliviero Barbara , Dei Cas Michele , Zulueta Aida , Maiello Roberta , Villa Alessandro , Martinelli Carla , Del Favero Elena , Falleni Monica , Montavoci Linda , Varchetta Stefania , Mele Dalila , Donadon Matteo , Soldani Cristiana , Franceschini Barbara , Maestri Marcello , Piccolo Gaetano , Barabino Matteo , Bianchi Paolo Pietro , Banales Jesus M. , Mantovani Stefania , Mondelli Mario U. , Caretti Anna 
Published: May 2023

AbstractCholangiocarcinoma (CCA) is a rare cancer characterized by a global increasing incidence. Extracellular vesicles (EV) contribute to many of the hallmarks of cancer through transfer of their cargo molecules. The sphingolipid (SPL) profile of intrahepatic CCA (iCCA)-derived EVs was characterized by liquid chromatography-tandem mass spectrometry analysis. The effect of iCCA-derived EVs as mediators of inflammation was assessed on monocytes by flow cytometry. iCCA-derived EVs showed downregulation of all SPL species. Of note, poorly-differentiated iCCA-derived EVs showed a higher ceramide and dihydroceramide content compared with moderately-differentiated iCCA-derived EVs. Of note, higher dihydroceramide content was associated with vascular invasion. Cancer-derived EVs induced the release of pro-inflammatory cytokines in monocytes. Inhibition of synthesis of ceramide with Myriocin, a specific inhibitor of the serine palmitoyl transferase, reduced the pro-inflammatory activity of iCCA-derived EVs, demonstrating a role for ceramide as mediator of inflammation in iCCA. In conclusion, iCCA-derived EVs may promote iCCA progression by exporting the excess of pro-apoptotic and pro-inflammatory ceramides.
The inflammatory prognostic index as a potential predictor of prognosis in metastatic gastric cancer
Authors: Ozveren Ahmet , Erdogan Atike Pinar , Ekinci Ferhat 
Published: May 2023

AbstractClinical studies aimed at identifying effective and simple prognostic markers for gastric cancer are still being carried out. Inflammatory prognostic index (IPI) is being recognized as a promising prognostic marker in patients with Non-Small Cell Lung Cancer. To evaluate the prognostic utility of IPI in stage 4 gastric cancer. A total of 152 patients with stage 4 gastric cancer, whose laboratory parameters, progression-free survival (PFS) and overall survival (OS) data could be accessed, were evaluated. Kaplan Meier analysis was used for survival analyses. Hazard ratios were expressed with 95% CI values. All methods were performed in accordance with the relevant guidelines and regulations. Study was approved by the Manisa Celal Bayar University’s Non-Invasive Clinical Research Ethics Committee (approval No. E-85252386-050.04.04-49119, date: 22.03.2021). We confirm that all methods were performed in accordance with relevant named guidelines and regulations. Median age at diagnosis was 63 years (range: 32–88). The number of patients who received first-line chemotherapy was 129 (84.9%). Median PFS with first-line treatment was 5.3 months, while it was 3.3 months with second-line treatment. Median OS was 9.4 months. Median IPI score was 22.2. We evaluated IPI score for its value in detecting survival status with ROC analysis and identified an IPI cut-off score of 14.6. Low IPI score was significantly associated with longer PFS and OS compared to high IPI (PFS in high vs. low IPI, 3.6 vs. 7 months; p < 0.001) (OS in high vs. low IPI, 6.6 vs. 14.2 months; p < 0.001). IPI score can be an independent prognostic index that is inexpensive, easy to access and evaluate for patients with metastatic gastric cancer, and may be useful in predicting survival in daily practice.
Variation along P2RX7 interacts with early traumas on severity of anxiety suggesting a role for neuroinflammation
Authors: Kristof Zsuliet , Gal Zsofia , Torok Dora , Eszlari Nora , Sutori Sara , Erdelyi-Hamza Berta , Petschner Peter , Sperlagh Beata , Anderson Ian M. , Deakin John Francis William , Bagdy Gyorgy , Juhasz Gabriella , Gonda Xenia 
Published: May 2023

AbstractEmotional stress is a leading risk factor in the development of neuropsychiatric disorders possibly via immune activation. P2X7 receptors promote neuroinflammation, and research suggests a relationship between chromosome region 12q2431, in which the P2X7R gene is located, and development of mood disorders, however, few studies concentrate on its association with anxiety. Our aim was to investigate the effects of P2RX7 variation in interaction with early childhood traumas and recent stressors on anxiety. 1752 participants completed questionnaires assessing childhood adversities and recent negative life events, provided data on anxiety using the Brief Symptom Inventory, and were genotyped for 681 SNPs in the P2RX7 gene, 335 of which passed quality control and were entered into linear regression models followed by a linkage disequilibrium-based clumping procedure to identify clumps of SNPs with a significant main or interaction effect. We identified a significant clump with top SNP rs67881993 and containing a set of 29SNPs that are in high LD, which significantly interacted with early childhood traumas but not with recent stress conveying a protective effect against increased anxiety in those exposed to early adversities. Our study demonstrated that P2RX7 variants interact with distal and more etiological stressors in influencing the severity of anxiety symptoms, supporting previous scarce results and demonstrating its role in moderating the effects of stress.
Egyptian propolis extract for functionalization of cellulose nanofiber/poly(vinyl alcohol) porous hydrogel along with characterization and biological applications
Authors: Saleh Safaa , Salama Ahmed , Ali Amira M. , Saleh Ahmed K. , Elhady Bothaina Abd , Tolba Emad 
Published: May 2023

AbstractBee propolis is one of the most common natural extracts and has gained significant interest in biomedicine due to its high content of phenolic acids and flavonoids, which are responsible for the antioxidant activity of natural products. The present study report that the propolis extract (PE) was produced by ethanol in the surrounding environment. The obtained PE was added at different concentrations to cellulose nanofiber (CNF)/poly(vinyl alcohol) (PVA), and subjected to freezing thawing and freeze drying methods to develop porous bioactive matrices. Scanning electron microscope (SEM) observations displayed that the prepared samples had an interconnected porous structure with pore sizes in the range of 10–100 μm. The high performance liquid chromatography (HPLC) results of PE showed around 18 polyphenol compounds, with the highest amounts of hesperetin (183.7 µg/mL), chlorogenic acid (96.9 µg/mL) and caffeic acid (90.2 µg/mL). The antibacterial activity results indicated that both PE and PE-functionalized hydrogels exhibited a potential antimicrobial effects against Escherichia coli, Salmonella typhimurium, Streptococcus mutans, and Candida albicans. The in vitro test cell culture experiments indicated that the cells on the PE-functionalized hydrogels had the greatest viability, adhesion, and spreading of cells. Altogether, these data highlight the interesting effect of propolis bio-functionalization to enhance the biological features of CNF/PVA hydrogel as a functional matrix for biomedical applications.
Machine learning-guided determination of Acinetobacter density in waterbodies receiving municipal and hospital wastewater effluents
Authors: Ekundayo Temitope C. , Adewoyin Mary A. , Ijabadeniyi Oluwatosin A. , Igbinosa Etinosa O. , Okoh Anthony I. 
Published: May 2023

AbstractA smart artificial intelligent system (SAIS) for Acinetobacter density (AD) enumeration in waterbodies represents an invaluable strategy for avoidance of repetitive, laborious, and time-consuming routines associated with its determination. This study aimed to predict AD in waterbodies using machine learning (ML). AD and physicochemical variables (PVs) data from three rivers monitored via standard protocols in a year-long study were fitted to 18 ML algorithms. The models’ performance was assayed using regression metrics. The average pH, EC, TDS, salinity, temperature, TSS, TBS, DO, BOD, and AD was 7.76 ± 0.02, 218.66 ± 4.76 µS/cm, 110.53 ± 2.36 mg/L, 0.10 ± 0.00 PSU, 17.29 ± 0.21 °C, 80.17 ± 5.09 mg/L, 87.51 ± 5.41 NTU, 8.82 ± 0.04 mg/L, 4.00 ± 0.10 mg/L, and 3.19 ± 0.03 log CFU/100 mL respectively. While the contributions of PVs differed in values, AD predicted value by XGB [3.1792 (1.1040–4.5828)] and Cubist [3.1736 (1.1012–4.5300)] outshined other algorithms. Also, XGB (MSE = 0.0059, RMSE = 0.0770; R2 = 0.9912; MAD = 0.0440) and Cubist (MSE = 0.0117, RMSE = 0.1081, R2 = 0.9827; MAD = 0.0437) ranked first and second respectively, in predicting AD. Temperature was the most important feature in predicting AD and ranked first by 10/18 ML-algorithms accounting for 43.00–83.30% mean dropout RMSE loss after 1000 permutations. The two models' partial dependence and residual diagnostics sensitivity revealed their efficient AD prognosticating accuracies in waterbodies. In conclusion, a fully developed XGB/Cubist/XGB-Cubist ensemble/web SAIS app for AD monitoring in waterbodies could be deployed to shorten turnaround time in deciding microbiological quality of waterbodies for irrigation and other purposes.
FOXK1 regulates malignant progression and radiosensitivity through direct transcriptional activation of CDC25A and CDK4 in esophageal squamous cell carcinoma
Authors: Li Xiaoxu , Lu Juntao , Liu Lei , Li Fei , Xu Tongxin , Chen Liying , Yan Zhaoyang , Li Yan , Guo Wei 
Published: May 2023

AbstractEsophageal squamous cell carcinoma (ESCC) is a serious malignancy with poor prognosis, necessitating identification of oncogenic mechanisms for novel therapeutic strategies. Recent studies have highlighted the significance of the transcription factor forkhead box K1 (FOXK1) in diverse biological processes and carcinogenesis of multiple malignancies, including ESCC. However, the molecular pathways underlying FOXK1’s role in ESCC progression are not fully understood, and its potential role in radiosensitivity remains unclear. Here, we aimed to elucidate the function of FOXK1 in ESCC and explore the underlying mechanisms. Elevated FOXK1 expression levels were found in ESCC cells and tissues, positively correlated with TNM stage, invasion depth, and lymph node metastasis. FOXK1 markedly enhanced the proliferative, migratory and invasive capacities of ESCC cells. Furthermore, silencing FOXK1 resulted in heightened radiosensitivity by impeding DNA damage repair, inducing G1 arrest, and promoting apoptosis. Subsequent studies demonstrated that FOXK1 directly bound to the promoter regions of CDC25A and CDK4, thereby activating their transcription in ESCC cells. Moreover, the biological effects mediated by FOXK1 overexpression could be reversed by knockdown of either CDC25A or CDK4. Collectively, FOXK1, along with its downstream target genes CDC25A and CDK4, may serve as a promising set of therapeutic and radiosensitizing targets for ESCC.
Physical conditions for dust grain alignment in Class 0 protostellar cores
Authors: Le Gouellec V. J. M. , Maury A. J. , Hull C. L. H. , Verliat A. , Hennebelle P. , Valdivia V. 
Published: May 2023

Context. The polarized dust emission observed in Class 0 protostellar cores at high angular resolution with ALMA has raised several concerns about the grain alignment conditions in these regions.
Aims. We aim to study the role of the radiation field in grain alignment mechanisms that occur in the interior (≤1000 au) of Class 0 protostars.
Methods. We produced synthetic observations of the polarized dust emission from a magnetohydrodynamic model of protostellar formation using the POLARIS dust radiative transfer tool, which includes dust alignment with radiative torque alignment (RAT). We tested how the polarized dust emission from the model core depends on the irradiation conditions in the protostellar envelope by varying the radiation due to accretion luminosity propagating from the central protostellar embryo throughout the envelope. The level of grain alignment efficiency obtained in the radiative transfer models was then compared to (sub)millimeter ALMA dust polarization observations of Class 0 protostars.
Results. Our radiative transfer calculations have a central irradiation that reproduces the protostellar luminosities typically observed toward low- to intermediate-mass protostars, as well as super-paramagnetic grains and grains ≥10 µm, which are required to bring the dust grain alignment efficiencies of the synthetic observations up to the observed levels. We discuss the characteristics timescales of the grain alignment physics together with the radiative torque disruption (RATD) of grains and the typical time variability of accretion occurring in Class 0 protostellar cores. In our model, during an accretion burst or a steady-state phase of high luminosity from the protostellar embryo, RATD could have enough time to disrupt the largest grains in irradiated regions. Finally, in high-luminosity conditions (with L★ ≥ 20 L⊙ in our model), we find that the alignment of grains with respect to the anisotropic component of the radiation field (k-RAT) could drive inefficient alignment for grains ≳10 µm. However, given the high grain alignment efficiency observed in protostellar envelopes, large grains are most likely aligned with the magnetic field and thus potentially subject to rotational disruption, depending on their tensile strength.
Conclusions. Our radiative transfer calculations show that irradiation plays an important role in the mechanisms that dictate the size range of aligned grains in Class 0 protostars. Regions of the envelope that are preferentially irradiated harbor strong polarized dust emission but can be affected by the rotational disruption of dust grains, thus controlling the population of the largest aligned grains. Episodes of high luminosity could affect grain alignment and trigger grain disruption mechanisms.
Slow-mode rarefaction and compression fronts in the Hermean magnetosphere: From MESSENGER insights to future BepiColombo observations
Authors: Varela J. , Pantellini F. 
Published: May 2023

Context. Standing slow-mode rarefaction and compression front structures may appear in the Mercury magnetosheath under particular solar wind conditions.
Aims. The aim of the study is to identify the wind conditions required for the formation of slow-mode structures (SMS) in the Mercury magnetosphere by comparing MESSENGER magnetometer data and magnetohydrodynamics simulations.
Methods. We used the magnetohydrodynamics code PLUTO in spherical coordinates to reproduce the interaction of the solar wind with the Mercury magnetosphere. First, the optimal wind conditions for the SMS formation were identified with respect to the orientation of the interplanetary magnetic field (IMF) and dynamic pressure. Next, the magnetic field calculated in the simulations along the MESSENGER trajectory was compared to MESSENGER magnetometer data to identify tracers of the satellite encounter with the SMS.
Results. Optimal wind conditions for the formation of SMS require that the IMF is oriented in the northward or radial directions. The MESSENGER orbit on 8th September 2011 takes place during wind conditions that are close to the optimal configuration for SMS formation near the north pole, leading to the possible intersection of the satellite trajectory with the SMS. MESSENGER magnetometer data show a rather strong decrease in the magnetic field module after the satellite crossed nearby the compression front that is observed in the simulation, providing indirect evidence of the SMS.