bluuebunny/crossref_metadata_2025_split huggingface dataset:
Head‐to‐head comparison of genotyping of human papillomavirus by GP5+/6+‐PCR‐based reverse dot blot hybridization assay and SPF10‐PCR‐based line probe assay
Authors: Yin Jian , Peng Siying , Li Xinyue , Zhang Changning , Hu Fangfang , Chen Wen , Qiao Youlin 
Published: December 2022

AbstractThe SPF10‐PCR‐based line probe assay (LiPA‐25) for human papillomavirus (HPV) genotyping with high analytical sensitivity and specificity was widely used in HPV vaccine clinical trials and epidemiologic studies. In the study, we aimed to compare a novel GP5+/6+‐PCR‐based reverse dot blot hybridization assay (Yaneng‐23) with LiPA‐25. The performance of two assays was evaluated in 1735 cervical swab and 117 tissue samples, with a focus on 19 common HPV types (14 high‐risk: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68/73; 5 low‐risk: 6, 11, 42, 43, and 53). A total of 1197 (69.0%) swab samples were identified as HPV‐positive by two assays. Of these, 878 (73.4%) samples displayed absolute agreement (concordant), 255 (21.3%) showed additional or fewer types (compatible), and the remaining 64 (5.3%) samples were discordant. Additionally, the two assays showed an excellent strength of agreement for 19 HPV‐combined detection (κ = 0.886) and 17 individual HPV types (all κ > 0.800), and displayed a good agreement for HPV39 (κ = 0.780) and 42 (κ = 0.699). Yaneng‐23 was more sensitive than LiPA‐25 for HPV58, 59, 68/73, 42, 43 and 53 (McNemar's test: all p < 0.05), while LiPA‐25 was more sensitive for HPV31, 39, 52, and 66 than Yaneng‐23 (all p < 0.05). In 113 HPV‐positive tissue specimens, the identification of genotypes was 82.3% identical and 17.7% compatible. The agreement between the tests for HPV45 (κ = 0.796) and 51 (κ = 0.742) was good, and for other types (all κ > 0.843) and 19 HPV‐combined detection (κ = 0.929) was perfect (all p > 0.05). In conclusion, Yaneng‐23 and LiPA‐25 are comparable. Yaneng‐23 could be used for the detection and genotyping of HPV in cervical samples for epidemiological and vaccine studies worldwide.
Fabrication of glycated yeast cell protein via Maillard reaction for delivery of curcumin: improved environmental stability, antioxidant activity, and bioaccessibility
Authors: Fu Jing‐jing , Fu Dong‐wen , Zhang Guang‐yao , Zhang Zhi‐hui , Xu Xian‐bing , Song Liang 
Published: December 2022

AbstractBACKGROUNDThe application of curcumin (CUR) in the food industry is limited by its instability, hydrophobicity and low bioavailability. Yeast cell protein (YCP) is a by‐product of spent brewer's yeast, which has the potential to deliver bioactive substances. However, the environmental stresses such as pH, salt and heat treatment has restricted its application in the food industry. Maillard reaction as a non‐enzymatic browning reaction can improve protein stability under environmental stress.RESULTSThe CUR was successfully encapsulated into the hydrophobic core of YCP/glycated YCP (GYCP) and enhanced by hydrogen bonding, resulting in static fluorescence quenching of YCP/GYCP. The average diameter and dispersibility of GYPC−CUR nanocomplex were significantly improved after glucose glycation (121.40 nm versus 139.70 nm). Moreover, the encapsulation capacity of CUR was not influenced by glucose glycation. The oxidative stability and bioaccessibility of CUR in nanocomplexes were increased compared with free CUR, especially complexed with GYCP conjugates.CONCLUSIONSteric hindrance provided by glucose conjugation improved the enviriomental stability, oxidative activity and bioaccessibility of CUR in nanocomplexes. Thus, glucose‐glycated YCP has potential application as a delivery carrier for hydrophobic compounds in functional foods. © 2022 Society of Chemical Industry.
Vocal Fold Pathologies Among Undergraduate Singing Students In Three Different Genres
Authors: Bretl Michelle M. , Gerhard Julia , Rosow David E. , Anis Mursalin , Landera Mario A. , Libman Dana , Marchman Judy , Ragsdale Frank , Moore Stephannie , Ma Ruixuan , Hoffman Bari , Ivey Chandra , Johns Michael M. , Menton Stacey M. , Lloyd Adam T. 
Published: December 2022

ObjectiveThis study aimed to compare the prevalence and incidence of vocal fold pathologies among undergraduate classical, musical theatre, and contemporary commercial music (CCM) students over two‐time points.MethodsThis study is part of a longitudinal investigation. Videostroboscopic examinations were rated, with consensus among three of four expert blinded raters confirming the presence of pathology. Association between genre of singer and the presence of pathology, interrater reliability, and intra‐rater reliability were calculated. Prevalence and incidence of pathologies were compared across genres.ResultsDuring first‐year evaluations, 32% of musical theatre, 18% of CCM, and 0% of classical students had vocal pathologies. The prevalence at third‐year evaluations showed 22% of classical, 39% of musical theatre, and 27% of CCM participants having vocal fold pathologies. The incidence of pathologies was 67% of musical theatre students compared to 22% of classical students and 27% of CCM students. The four raters demonstrated fair to moderate interrater agreement. Singing Voice Handicap Index‐10 scores were normal for CCM singers at both time points but elevated for musical theatre and classical singers.ConclusionNo classical singers were found to have pathology during first‐year evaluations, although CCM and musical theatre singers showed evidence of vocal fold pathologies. At third‐year evaluations, all three genres had an apparent increase in prevalence of pathologies. Implications of this study suggest that more time in the field and intense voice usage may lead to a greater risk of pathology for all singers, regardless of genre.Level of Evidence2 Laryngoscope, 133:2317–2324, 2023
Adoption of otolaryngologist‐head neck surgeons toward transoral robotic surgery: An international survey
Authors: Lechien Jerome R. , Haddad Leonardo , Holsinger F. Christopher , Mendelsohn Abie H. , Hans Stephane 
Published: December 2022

AbstractObjectiveTo investigate perception, adoption and awareness of otolaryngologist‐head neck surgeons (OTO‐HNS) toward transoral robotic surgery (TORS).MethodsAn online survey was sent to 1383 OTO‐HNS on the perception, adoption and awareness about TORS to members of many otolaryngological societies. The following aspects were assessed: TORS access; training; awareness/perception; indications and advantages/barriers to TORS practice. The responses were presented for the entire cohort and regarding the TORS experience of OTO‐HNS.ResultsA total of 359 completed the survey (26%); including 115 TORS surgeons. TORS‐surgeons carry out a mean number of 34.4 annual TORS procedures. The primary barriers to TORS were the cost of the robot (74%) and disposable accessories (69%), and the lack of training opportunity (38%). The 3D view of the surgical field (66%), the postoperative quality of life outcomes (63%) and the shorter hospital stay (56%) were the most important benefits of TORS. TORS‐surgeons believed more frequently that TORS is indicated for cT1‐T2 oropharyngeal and supraglottic cancers than non‐TORS surgeons (p < .005). Participants believed that the priorities for the future consisted of the reduction of the robot arm size and the incorporation of flexible instruments (28%); the integration of laser (25%) or GPS tracking based on imaging (18%), all of them to improve accesses to hypopharynx (24%), supraglottic larynx (23%) and vocal folds (22%).ConclusionsThe perception, adoption and knowledges toward TORS depend on the access to robot. The findings of this survey may help guide decisions on how improve the dissemination of TORS interest and awareness.
Dark Singlet Exciton Sensitized Triplet Energy Transfer Across the CsPbBr3 Nanoplate‐Organic Interface
Authors: Xu Kunyuan , Li Renfu , Chen Dejian , Hu Jie , Liang Sisi , Zhu Haomiao 
Published: December 2022

AbstractSemiconductor nanocrystals (NCs) sensitized triplet energy transfer (TET) provides a promising alternative for achieving efficient triplet‐triplet annihilation up‐conversion (TTA‐UC). However, the study on the role of NC's exciton fine structure in TET, which is important to further enhance the TET efficiency, is still limited. Herein, the respective contribution of bright/dark exciton to TET using 1‐naphthoic acid (mediator) decorated 2 nm thick CsPbBr3 nanoplates as the model system is studied. The well‐characterized and unique exciton fine structure and large bright‐dark splitting energy of CsPbBr3 nanoplates provide an ideal platform to unambiguously explore the TET between dark singlet exciton and mediator. By using time‐resolved temperature dependent optical spectroscopy, the direct participation of dark singlet state in the TET process is proved and also point out that the dark singlet exciton to mediator TET is the major energy transfer pathway, which is due to the ill‐defined nature of exciton's spin in strongly confined nanoplates and dark exciton's long intrinsic lifetime. Finally, in combination with 2,5‐diphenyloxazole as annihilator, mediator decorated CsPbBr3 nanoplates can convert visible to ultraviolet light with a maximum photon gain of 0.75 eV under a low threshold of ≈0.76 W cm−2.
An in‐house 45‐plex array for the detection of antimicrobial resistance genes in Gram‐positive bacteria
Authors: Kowalewicz Carole , Timmermans Michael , Fretin David , Wattiau Pierre , Boland Cécile 
Published: December 2022

AbstractIdentifying antimicrobial resistance (AMR) genes and determining their occurrence in Gram‐positive bacteria provide useful data to understand how resistance can be acquired and maintained in these bacteria. We describe an in‐house bead array targeting AMR genes of Gram‐positive bacteria and allowing their rapid detection all at once at a reduced cost. A total of 41 AMR probes were designed to target genes frequently associated with resistance to tetracycline, macrolides, lincosamides, streptogramins, pleuromutilins, phenicols, glycopeptides, aminoglycosides, diaminopyrimidines, oxazolidinones and particularly shared among Enterococcus and Staphylococcus spp. A collection of 124 enterococci and 62 staphylococci isolated from healthy livestock animals through the official Belgian AMR monitoring (2018–2020) was studied with this array from which a subsample was further investigated by whole‐genome sequencing. The array detected AMR genes associated with phenotypic resistance for 93.0% and 89.2% of the individual resistant phenotypes in enterococci and staphylococci, respectively. Although linezolid is not used in veterinary medicine, linezolid‐resistant isolates were detected. These were characterized by the presence of optrA and poxtA, providing cross‐resistance to other antibiotics. Rarer, vancomycin resistance was conferred by the vanA or by the vanL cluster. Numerous resistance genes circulating among Enterococcus and Staphylococcus spp. were detected by this array allowing rapid screening of a large strain collection at an affordable cost. Our data stress the importance of interpreting AMR with caution and the complementarity of both phenotyping and genotyping methods. This array is now available to assess other One‐Health AMR reservoirs.
Clinical Impression of Severity Index for Parkinson's Disease and Its Association to Health‐Related Quality of Life
Authors: Norlin Jenny M. , Kellerborg Klas , Persson Ulf , Åström Daniel Oudin , Hagell Peter , Martinez‐Martin Pablo , Odin Per 
Published: December 2022

ABSTRACTBackgroundClinical Impression of Severity Index for Parkinson's Disease (CISI‐PD) is a simple tool that can easily be used in clinical practice. Few studies have investigated the relationship between health‐related quality of life and the CISI‐PD.ObjectiveTo analyze the association of CISI‐PD scores with those of generic (EQ‐5D‐5L) and Parkinson's disease (PD) disease‐specific (Parkinson's Disease Questionnaire–8 [PDQ‐8]) health‐related quality of life assessments.MethodsPersons with idiopathic PD in the Swedish Parkinson's Disease registry with simultaneous registrations of CISI‐PD and EQ‐5D‐5L and/or PDQ‐8 were included. Correlations with EQ‐5D dimensions were analyzed. The relationships between the CISI‐PD, EQ‐5D‐5L, and PDQ‐8 were estimated by linear mixed models with random intercept.ResultsIn the Swedish Parkinson's Disease registry, 3511 registrations, among 2168 persons, fulfilled the inclusion criteria. The dimensions self‐care, mobility, and usual activities correlated moderately with the CISI‐PD (rs = 0.60, rs = 0.54, rs = 0.57). Weak correlations were found for anxiety/depression and pain/discomfort (rs = 0.39, rs = 0.29) (P values < 0.001). The fitted model included the CISI‐PD, age, sex, and time since diagnosis. The CISI‐PD had a statistically significant impact on the EQ‐5D and PDQ‐8 (P values < 0.001).ConclusionsThe CISI‐PD provides a moderate correlation with the EQ‐5D and could possibly be useful as a basis for defining health states in future health economic models and serving as outcomes in managed entry agreements. Nonetheless, the limitation of capturing nonmotor symptoms of the disease remains a shortcoming of clinical instruments, including the CISI‐PD.
Analysis of decoupling performances of an isolation barrier using a mode‐matching method
Authors: Park Jae‐Yoon , Choo Jaeyul 
Published: December 2022

AbstractThis paper uses a mode‐matching method to analyze the decoupling performance of an isolation barrier that prevents electromagnetic interference between cables. After dividing the overall analyzed space into multiple subregions, potential expressions for each subregion were derived using the Laplace equation in conjunction with the superposition principle. The Dirichlet and Neumann boundary conditions were then applied to the interfaces between subregions to compute the modal coefficients in the previously derived potential expressions. Based on the electric‐field strengths and distributions used in our testing, the geometrical parameters and voltage conditions were varied to find the optimal configuration of the isolation barrier and cables that maximize decoupling performance. The performed study is novel and informative in that the analysis results provide the electromagnetic background to determine the distances between cables separated by an isolation barrier in industrial facilities such as nuclear power plants.
How did the COVID‐19 pandemic affect urinary incontinence and its management in the nursing homes? A descriptive phenomenological study
Authors: Yildirim Meltem , Minobes‐Molina Eduard , Oliveira Vinicius Rosa , Coll‐Planas Laura , Moreno‐Martin Pau , Rierola‐Fochs Sandra , Booth Joanne , Jerez‐Roig Javier 
Published: December 2022

AbstractBackgroundManagement of urinary incontinence (UI) in nursing homes (NHs) represents a complex process that may have become more challenging during a period in which front‐line health professionals (HPs) must deal with the outbreak of a new infectious disease. We aimed to investigate how UI and its management was affected in NHs during the COVID‐19 pandemic based on the perception of health professionals.MethodsThis qualitative study was conducted between January and March 2021 with 16 health professionals working in nine NHs in the Osona county (Barcelona, Spain) during the COVID‐19 pandemic. The individual interviews were conducted via online videoconferencing and each session was audio recorded.ResultsThe HPs highlighted that the COVID‐19 pandemic affected the management of continence in NHs, resulting in increased UI. Two main factors were identified: workforce‐related and resident‐related factors. Considering the workforce, three main topics emerged: pandemic as the priority, lack of personnel and use of personal protective equipment as an obstacle to communication with residents and attending to their needs.ConclusionManaging UI in NHs always has been a challenging point of care, and these challenges were worsened by the restrictions and additional difficulties that arose during the COVID‐19 pandemic.
Parental involvement in postoperative pain management among children in a urology ward: A best practice implementation project
Authors: Yang Jin‐xia , Zhang Wen‐yan , Huang Huan‐huan , Jiang Wen‐ting , Zhou Yun‐feng , Gu Ying , Xu Hong‐zhen , Yao Wen‐ying , Zhang Fang 
Published: December 2022

AbstractAimPostoperative pain has adverse effects on children with urological problems, including sleep disturbances, incision dehiscence, bleeding and delayed recovery. Accurate parental assessment of children's behaviours and responses could help to manage postoperative pain. We aimed to implement evidence‐based practice for parental involvement in a urology ward, to increase parents' participation in children's postoperative pain management.DesignThe project was conducted in a paediatric urology ward using the framework and methods of the Fudan University Evidence‐Based Nursing Center's Evidence‐based Continuous Quality Improvement Model.MethodsFifteen audit criteria were used to represent best practice recommendations for parental involvement in postoperative pain management. A pre‐implementation audit was conducted with 211 randomly sampled children and parents. Obstacles, promoting factors and key strategies were analysed, and evidence‐based interventions implemented to improve compliance. A follow‐up audit using the same audit criteria was conducted with 202 children and parents to assess the effect of targeted strategies on compliance with best practice. The SQUIRE guidelines were followed.ResultsAt the baseline audit, compliance with the evidence‐based criteria was 0%–71.5%; only five audit criteria achieved a compliance rate > 60%. After best practice implementation, the follow‐up audit showed compliance improvements for all criteria; compliance for three criteria improved to 100%.Patient or Public ContributionThis best practice implementation project improved parents' participation in children's postoperative pain management. The findings demonstrate how audits can promote best practice in postoperative pain management for children. Additional studies will be conducted to address children's postoperative life quality based on best practice.
Preferential flow between rivers and aquifers in alluvial floodplains: A key to modelling and sustainably managing shallow groundwater resources
Authors: Martin J. Michael , Everett Mark E. , Knappett Peter S. K. , Ewing Ryan C. 
Published: December 2022

AbstractPreferential flow between rivers and aquifers in alluvial floodplains may be a core component of shallow groundwater transport and, consequently, its understanding is key to modelling and managing groundwater resources. At a clay wedge separating present‐day streamflow and bank storage from an adjacent shallow aquifer, we image a suspected sand‐dominated structure. This structure cuts through the clay wedge and possesses temporally dynamic electrical resistivity as seen in time‐lapse electrical resistivity tomographic (ERT) images collected over a 61‐day study period. During days 11–12, following heavy rainstorms, the cross section of the electrically resistive sand fades into the background resistivity structure, reappearing the following day. This research shows that preferential flow can be imaged in time‐lapse ERT in buried sand‐dominated structures between a floodplain and the adjacent river. Our analysis demonstrates that sand conduits can transport infiltrated rainwater from the floodplain into the river as a bank spring and, hypothetically, at high‐stage streamflow, from the river into the adjacent shallow aquifer. In both directions, these conduits for preferential flow exert an important control on the regulation and distribution of water, sediments and contaminants. This phenomenon will help hydrological models to incorporate more real‐world phenomena and ultimately better prepare groundwater managers to sustainably steward shallow groundwater resources.
Solid‐phase extraction based on PDMS/ionic liquid sponge followed by gas chromatography–mass spectrometry for rapid and sensitive determination of volatile components in lavender
Authors: Wang Lili , Fu Jihong , Jiang Xinxing , Li Dandan 
Published: December 2022

AbstractIntroductionDue to the complexity and the low concentrations of volatile components in aromatic plants, sample pretreatment is an important step in the whole analytical procedure.ObjectiveThis study aimed to propose a novel, sensitive and fast approach to determine the volatile components in lavender.MethodsThe 1‐butyl‐3‐(propyltrimethoxysilane)imidazolium chloride ([BPtmsim]Cl) ionic liquid was introduced onto the surface of polydimethylsiloxane (PDMS) to prepare a novel PDMS/[BPtmsim]Cl sponge with large surface area, good sorption performance, and reusability. A solid‐phase extraction method was developed based on PDMS/[BPtmsim]Cl sponge combined with gas chromatography–mass spectrometry (GC–MS).ResultsThe effects of the various experimental parameters on the extraction efficiency were investigated. The optimal conditions were [BPtmsim]Cl amount of 0.3 g, 1:4 as the mass ratio of PDMS/[BPtmsim]Cl to lavender sample, microwave power of 700 W, microwave time of 10 min, and n‐hexane as the desorption solvent. The method validation results showed good linearity (10–800 μg/ml), high correlation coefficient (R2 ≥ 0.9991), low limits of detection (1.73–2.50 ng/μl), and limits of quantification (4.10–5.11 ng/μl). The interday and intraday precision with relative standard deviation (RSD) values were below 1.93% and 4.71%, respectively. Under the optimal extraction conditions, 16 lavender samples from three different species were analysed and a total of 57 volatile compounds were identified. The correlation between different species of lavender and volatile components was explored using principal component analysis (PCA) and partial least squares‐discriminant analysis (PLS‐DA).ConclusionThe results showed that PDMS/[BPtmsim]Cl extraction is a rapid, highly efficient, and sensitive technique for the determination of volatile components in complex plant samples.
Intraoperative plasma proteomic changes in cardiac surgery: In search of biomarkers of post‐operative delirium
Authors: Wiredu Kwame , O'Connor Sean , Naseem Heba , Brauer Brooke L. , Kettenbach Arminja N. , Frost Hildreth R. , Shaefi Shahzad , Gerber Scott A. 
Published: December 2022

AbstractPurposeDelirium presents a significant healthcare burden. It complicates post‐operative care in up to 50% of cardiac surgical patients with worse outcomes, longer hospital stays and higher cost of care. Moreover, the nature of delirium following cardiac surgery with cardiopulmonary bypass (CPB) remains unclear, the underlying pathobiology is poorly understood, status quo diagnostic methods are subjective, and diagnostic biomarkers are currently lacking.ObjectiveTo identify diagnostic biomarkers of delirium and for insights into possible neuronal pathomechanisms.Experimental DesignComparative proteomic analyses were performed on plasma samples from a nested matched cohort of patients who underwent cardiac surgery. Validation by targeted proteomics was performed in an independent set of samples. Biomarkers were assessed for biological functions and diagnostic accuracy.ResultsForty‐seven percent of subjects demonstrated delirium. Of 3803 proteins identified from patient samples by multiplexed quantitative proteomics, 16 were identified as signatures of exposure to CPB, and 11 biomarkers distinguished delirium cases from non‐cases (AuROC = 93%). Notable among these biomarkers are C‐reactive protein, serum amyloid A‐1 and cathepsin‐B.Conclusions and Clinical RelevanceThe interplay of systemic and central inflammatory markers sheds new light on delirium pathogenesis. This work suggests that accurate identification of cases may be achievable using panels of biomarkers.