bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Money can buy health”
Authors: Tse Vincent Wai Sum , Wu Jasper Zhao Zhen , Theng Andre Joseph 
Published: July 2023

Abstract
Drawing on the notion of affect, this paper offers a multimodal critical discourse analysis of health-related
product advertising in Hong Kong during the early months of the COVID-19 pandemic. Advertisements were collected in the city’s
space of commute. We examine the construal of risk and protection in the advertisements and via their emplacement in three
spatial-temporal dimensions observed in the data: the market society, the concrete public space, and the intimate familial space.
Our analysis demonstrates that while risk and protection are linguistically and visually depicted to varying degrees across these
dimensions, the emplacement of advertisements prompts the viewers to regard their immediate space as full of risks. As individuals
encounter the advertisements, they become interpellated to see the purchase of the advertised products as necessary. Illuminating
health communication during the pandemic, this study contributes to health commercialisation and responsibilisation research and
provides insights into the ideological, interpersonal nature of affect.
Diploid and tetraploid cytotypes of the flagship Cape species Dicerothamnus rhinocerotis (Asteraceae): variation in distribution, ecological niche, morphology and genetics
Authors: Chumová Zuzana , Monier Zafar , Šemberová Kristýna , Havlíčková Eliška , Euston-Brown Douglas , Muasya A Muthama , Bergh Nicola G , Trávníček Pavel 
Published: July 2023

Abstract

Background and Aims
The Greater Cape Floristic Region is one of the world’s biodiversity hotspots and is considered poor in polyploids. To test this assumption, ploidy variation was investigated in a widespread Cape shrub, Dicerothamnus rhinocerotis (renosterbos, Asteraceae). The aim was to elucidate the cytotype distribution and population composition across the species range, and to assess differences in morphology, environmental niches and genetics.

Methods
Ploidy level and genome size were determined via flow cytometry and cytotype assignment was confirmed by chromosome counting. Restriction site-associated DNA sequencing (RADseq) analyses were used to infer genetic relationships. Cytotype climatic and environmental niches were compared using a range of environmental layers and a soil model, while morphological differences were examined using multivariate methods.

Key Results
The survey of 171 populations and 2370 individuals showed that the species comprises diploid and tetraploid cytotypes, no intermediates and only 16.8 % of mixed populations. Mean 2C values were 1.80–2.06 pg for diploids and 3.48–3.80 pg for tetraploids, with very similar monoploid genome sizes. Intra-cytotype variation showed a significant positive correlation with altitude and longitude in both cytotypes and with latitude in diploids. Although niches of both cytotypes were highly equivalent and similar, their optima and breadth were shifted due to differences mainly in isothermality and available water capacity. Morphometric analyses showed significant differences in the leaves and corolla traits, the number of florets per capitulum, and cypsela dimensions between the two cytotypes. Genetic analyses revealed four groups, three of them including both cytotypes.

Conclusions
Dicerothamnus rhinocerotis includes two distinct cytotypes that are genetically similar. While tetraploids arise several times independently within different genetic groups, morphological and ecological differences are evident between cytotypes. Our results open up new avenues for questions regarding the importance of ploidy in the megadiverse Cape flora, and exemplify the need for population-based studies focused on ploidy variation.
Seaweed forests are carbon sinks that may help mitigate CO2 emissions: a comment on Gallagher et al. (2022)
Authors: Filbee-Dexter Karen , Pessarrodona Albert , Duarte Carlos M , Krause-Jensen Dorte , Hancke Kasper , Smale Daniel , Wernberg Thomas 
Published: July 2023

Abstract
Recently, Gallagher et al. (2022) suggested that seaweed ecosystems are net heterotrophic carbon sources due to CO2 released from the consumption of external subsidies. Here we outline several flaws in their argument, which we believe confuse research on the blue carbon potential of seaweed ecosystems, and unjustifiably generate doubt around initiatives to protect and restore seaweed forests. Gallagher et al.’s evidence relies on 18 studies with highly variable measures of net ecosystem production, which do not statistically support their conclusion that most seaweed ecosystems are heterotrophic. This dataset is also inappropriate as it is incomplete and misrepresents seaweed ecosystems globally, particularly seaweed forests, which contribute disproportionately to global seaweed productivity. We maintain that the climate change mitigation value of an ecosystem depends on the net difference in CO2 uptake between the original ecosystem and its replacement ecosystem. We provide evidence that most seaweed ecosystems, which drawdown the largest carbon flux of any vegetated coastal habitat, are indeed net autotrophic ecosystems. We recognize that substantial uncertainties remain concerning the magnitude of CO2 drawdown by seaweed ecosystems and recommend that carbon fluxes around seaweed ecosystems should be considered more broadly and taken into account in estimates of their CO2 mitigation potential.
School Resource Officers: More than Just Law Enforcers?
Authors: Montes Andrea N , Trinkner Rick , Scott Michael S 
Published: July 2023

Abstract
Research about school resource officers (SROs) has focussed on their ability to legally intervene and detain or arrest youth. On many campuses, though, their role extends beyond that of law enforcement to include mentorship, counselling, and education. The current study draws on a survey of SROs from the Phoenix, Arizona, Police Department—one of the largest police departments in the United States—to examine what roles SROs prioritize and how they implement their duties. The results show that although one of SROs’ primary roles is that of law enforcer, SROs were more likely to prioritize their role as mentors. SROs also reported that it was school personnel, rather than teachers or the SROs themselves, who most commonly initiated SRO involvement in student misbehaviour, whether in a law enforcement or mentorship capacity. The paper concludes with a discussion about implications for scholarship and practice.
The Determinants of Taxation Innovation Policy in Spain’s Common Regime Autonomous Communities (1986–2018)
Authors: Kölling Mario , Toubeau Simon , Godoy Deborah Zak 
Published: July 2023

Abstract
This article studies the determinants of tax innovation in Spain’s Common Regime Autonomous Communities (ACs) over the period 1986–2018, across the different types of taxes included in their “own taxes.” Our central finding is that the introduction of taxes is motivated by politics: ACs governments introduce taxes when governed by left-wing parties or by a coalition government that included a regionalist party. Second, we find that parties in government follow a strategic calculus when introducing new taxes: an approaching election and a previously introduced tax decrease the chances of tax innovation. Third, we find that AC government also respond to functional pressures and introduce new taxes to shore-up their revenues when faced with a budget deficit. Two important negative results to come out of this analysis are that taxation innovation is not sensitive to geographical diffusion or to the availability of alternative source of revenue in the system of territorial financing.
Phylogenetic revision of whisker-cheeked suckermouth catfishes (Loricariidae: Lasiancistrus) from east of the Andes: five species where once there were two
Authors: Poveda-Cuellar José Luis , Conde-Saldaña Cristhian Camilo , Villa-Navarro Francisco Antonio , Lujan Nathan K , Dergam dos Santos Jorge Abdala 
Published: July 2023

Abstract
We integrated large sample sizes, morphometric and molecular data, and phylogenetic and species delimitation analyses to test the 17-year-old hypothesis that only two species of whisker-cheeked suckermouth catfishes (genus Lasiancistrus) occur in river drainages west of the Andes Mountains. Our results reject this hypothesis, demonstrating that, in addition to the previously recognized Lasiancistrus guacharote from Lake Maracaibo, a Lasiancistrus clade from west of the Sierra de Perijá contains at least four allopatric, genetically differentiated and morphologically distinct lineages. One of these lineages had no previous name associated with it and is described here as the new species Lasiancistrus wiwa. Phylogenetic relationships and geographical distributions of all five trans-Andean lineages are concordant with watershed boundaries and major mountain ranges that form these boundaries, with the following five freshwater basins or regions each containing a single species: Lake Maracaibo (L. guacharote), Rancheria River basin (L. wiwa), Upper and Middle Magdalena River and lower Cauca River basins (Lasiancistrus volcanensis), Upper Cauca River basin (Lasiancistrus caucanus) and Pacific Coastal watersheds between central Colombia and central Panama (Lasiancistrus mayoloi). Evolutionary relationships among these lineages suggest that Andean uplift-mediated vicariance contributed significantly to the cladogenesis and allopatric distributions of these fishes.
A systematic assessment of the impact of rare canonical splice site variants on splicing using functional andin silicomethods
Authors: Oh Rachel Y. , AlMail Ali , Cheerie David , Guirguis George , Hou Huayun , Yuki Kyoko E. , Haque Bushra , Thiruvahindrapuram Bhooma , Marshall Christian R. , Mendoza-Londono Roberto , Shlien Adam , Kyriakopoulou Lianna G , Walker Susan , Dowling James J. , Wilson Michael D. , Costain Gregory 
Published: July 2023

AbstractBackground/ObjectivesCanonical splice site variants (CSSVs) are often presumed to cause loss-of-function (LoF) and are assigned very strong evidence of pathogenicity (according to ACMG criterion PVS1). However, the exact nature and predictability of splicing effects of unselected rare CSSVs in blood-expressed genes is poorly understood.MethodsA total of 184 rare CSSVs in unselected blood-expressed genes were identified by genome sequencing in 121 individuals, and their impact on splicing was interrogated manually in RNA sequencing (RNA-seq) data. Blind to these RNA-seq data, we attempted to predict the precise impact of CSSVs by applyingin silicotools and the ClinGen Sequence Variant Interpretation Working Group 2018 guidelines for applying PVS1 criterion.ResultsThere was no evidence of a frameshift nor of reduced expression consistent with nonsense-mediated decay (NMD) for 24% of CSSVs: 17% had wildtype splicing only and normal junction depths, 3.25% resulted in cryptic splice site usage and in-frame indels, 3.25% resulted in full exon skipping (in-frame), and 0.5% resulted in full intron inclusion (in-frame). Misclassification rates for splicing outcome (frameshift/NMD vs. no frameshift/no NMD) using (i) SpliceAI, (ii) MaxEntScan, and (iii) AutoPVS1 ranged from 30-41%, with none outperforming a simple “zero rule” classifier.ConclusionNearly 1 in 4 CSSVs may not cause LoF based on analysis of RNA-seq data. Predictions fromin silicomethods were often discordant with findings from RNA-seq. More caution may be warranted in applying PVS1-level evidence to CSSVs in the absence of functional data.
Phase-locked auditory pulse stimulation at home enhancing slow sleep waves: A pilot real-world study
Authors: Xi Guannan , Zheng Xin , Gao Anchen , Huang Siyang , Lei Huijie , Ding Jing , Zhang Qianqian , Jiang Jian 
Published: July 2023

AbstractBackgroundDeep sleep, also known as slow-wave sleep (SWS), is essential for maintaining good health and is characterized by electroencephalographic (EEG) slow-wave activity (SWA). The use of phase-locked auditory stimulation (PLAS) to enhance SWA has emerged as a promising approach. However, the effectiveness of home-based PLAS has not yet been fully established.MethodWe used a newly developed wearable EEG device, called LANMAO, to record sleeping EEG signals and synchronize acoustic tones with SWA. We employed a within-subject design to investigate whether acoustic stimulation could increase SWA in a home setting using six subjects (mean age: 29±4.2 years, 3 males). Specifically, we applied acoustic stimulation (STIM) on odd-numbered slow waves and no stimulation (SHAM) on even-numbered slow waves.ResultsThe PLAS significantly enhanced SWA, theta, alpha, fast spindle and slow spindle activity on STIM condition compare to SHAM condition across all subjects in home setting.ConclusionThese results indicated that the capacity of PLAS, based on LANMAO device, could enhance the SWA in home setting. Our findings shed lights on the wide application of home-based deep sleep intervention.
Effect of Women’s View Toward Their Rights on Receiving Better Antenatal Care in Bangladesh
Authors: Morshed Ramisa 
Published: July 2023

AbstractBackgroundIn a country, many girls may forcefully be married off at an early age where they might have no hand in it. But the thought and view they carry throughout their lifetime can be resembled in their children’s lives too and can proclaim plenty of the country’s problems, especially receiving improved antenatal care (ANC). A woman or a prospective mother’s view of her husband beating her tells a lot about a country’s view, and a woman’s preferred age of marrying can also exhibit her attitude toward her rights and goals. However, limited research has focused on understanding women’s perspectives on their rights and their relation with ANC quality in Bangladesh. The findings of this study will contribute to a deeper understanding of women’s perspectives on their rights and the barriers they face in accessing quality ANC.ObjectiveThis study aims to investigate the view of Bangladeshi women toward their own rights and the impact on their access to improved ANC.MethodTo address this gap, a binary logistic regression model was employed by analyzing in IBM SPSS Statistics 25 using Bangladesh Demographic and Health Survey (BDHS) 2017-18 data. The study recruited a diverse sample of women from various social backgrounds, beliefs, and geographical locations in Bangladesh.ResultIt was spotted that a small percentage (6.6%) of women in Bangladesh were under proper ANC coverage. The women who didn’t justify domestic violence were 1.231 times more likely to be under ANC coverage in Bangladesh than those who justified domestic violence in the adjusted model. Moreover, women with no education were .048 times less likely to be under ANC coverage than women with higher education. Women who were not exposed to media were .099 times less likely to be under ANC coverage than those who were exposed to media. Unfortunately, almost one-fifth of all Bangladeshi women (19.6%) justified beingConclusionWomen’s entitlements enable them to actively engage in their own healthcare programs, leading to improved health outcomes for both mother and child. Through targeted awareness campaigns, educational initiatives, and policy advocacy, beaten by their husbands.Bangladesh can create an environment where women are aware of their rights and have access to quality ANC services. By ensuring their rights are respected, a society that values reproductive health and the well-being of women and children can strive.
Improved specificity of glutamate decarboxylase 65 autoantibody measurement using luciferase-based immunoprecipitation system (LIPS) assays
Authors: Wyatt R.C. , Grace S.L. , Brigatti C. , Marzinotto I. , Gillard B.T. , Shoemark D. , Chandler K. , Achenbach P. , Piemonti L. ,   The BOX Study Group, Long A.E. , Gillespie K.M. , Lampasona V. , Williams A.J.K. 
Published: July 2023

AbstractAutoantibodies to glutamate decarboxylase (GADA) are widely used in the prediction and classification of type 1 diabetes. GADA radiobinding assays (RBAs) using N-terminally truncated antigens offer improved specificity but radioisotopes limit the high-throughput potential for population screening. Luciferase-based Immunoprecipitation System (LIPS) assays are sensitive and specific alternatives to RBAs with the potential to improve risk stratification.The performance of assays using the Luciferase (Nluc-) conjugated GAD65constructs, Nluc-GAD65(96-585) and full length Nluc-GAD65(1-585) were evaluated in 434 well-characterised sera from recent-onset type 1 diabetes patients and first-degree relatives.Non-radioactive, high-throughput LIPS assays are quicker and require less serum than RBAs. Of 171 relatives previously tested single autoantibody positive for autoantibodies to full-length GAD65by RBA but had not progressed to diabetes, fewer retested positive by LIPS using either truncated (n=72) or full-length (n=111) antigen. The Nluc-GAD65(96-585) truncation demonstrated the highest specificity in LIPS assays overall but in contrast to RBA, N-terminus truncations did not result in a significant increase in disease-specificity compared with the full-length antigen. This suggests that binding of non-specific antibodies is affected by the conformational changes resulting from addition of the Nluc antigen. Nluc-GAD65(96-585) LIPS assays offer low blood volume, high specificity GADA tests for screening and diagnostics.
A comparison of critical speed and critical power in runners using Stryd running power
Authors: van Rassel C. R. , Sales M. K. , Ajayi O. O. , Nagai K. , MacInnis M. J. 
Published: July 2023

AbstractPurposeAlthough running traditionally relies on critical speed (CS) as an indicator of critical intensity, portable inertial measurement units (IMUs) offer a potential solution for estimating running mechanical power to assess critical power (CP) in runners. The purpose of this study was to determine whether CS and CP differ when assessed using the Stryd device, a portable IMU, and if two running bouts are sufficient to determine CS and CP.MethodsOn an outdoor running track, ten trained runners (, 59.0 [4.2] ml·kg-1·min-1) performed three running time-trials (TT) between 1200 and 4400m on separate days. CS and CP were derived from two-parameter hyperbolic speed-time and power-time models, respectively, using two (CS2TTCP2TT) and three (CS3TTCP3TT) time trials. Subsequently, runners performed constant intensity running for 800m at their calculated CS3TTand CP3TT.ResultsRunning at the calculated CS3TTspeed (3.88 [0.44] m·s-1) elicited an average Stryd running power (271 [28] W) not different from the calculated CP3TT(270 [28]; p=0.940; d=0.02), with excellent agreement between the two values (ICC=0.980). The CS2TT(3.97 [0.42] m·s-1) was not significantly higher than CS3TT(3.89 [0.44] m·s-1; p=0.178; d=0.46); however, CP2TT(278 [29] W) was significantly greater than CP3TT(p=0.041; d=0.75).ConclusionThe running intensities at CS and CP were similar, supporting the use of running power (Stryd) as a metric of aerobic fitness and exercise prescription, and two trials provided a reasonable, albeit higher, estimate of CS and CP.
Single-cell RNAseq identifies clonally expanded antigen-specific T-cells following intradermal injection of gold nanoparticles loaded with diabetes autoantigen in humans
Authors: Hanna Stephanie J. , Thayer Terri C. , Robinson Emma J.S. , Vinh Ngoc-Nga , Williams Nigel , Landry Laurie , Andrews Robert , Siah Qi Zhuang , Leete Pia , Wyatt Rebecca , McAteer Martina A. , Nakayama Maki , Wong F. Susan , Yang Jennie H.M. , Tree Tim I.M. , Ludvigsson Johnny , Dayan Colin M. , Tatovic Danijela 
Published: July 2023

ABSTRACTGold nanoparticles (GNPs) have been used in the development of novel therapies as a way of delivery of both stimulatory and tolerogenic peptide cargoes. Here we report that intradermal injection of GNPs loaded with the proinsulin peptide C19-A3, in patients with type 1 diabetes, results in recruitment and retention of immune cells in the skin. These include large numbers of clonally expanded T-cells sharing the same paired T-cell receptors (TCRs) with activated phenotypes, half of which, when the TCRs were re-expressed in a cell-based system, were confirmed to be specific for either GNP or proinsulin. All the identified gold-specific clones were CD8+, whilst proinsulin-specific clones were both CD8+and CD4+. Proinsulin-specific CD8+clones had a distinctive cytotoxic phenotype with overexpression of granulysin (GNLY) and KIR receptors. Clonally expanded antigen-specific T cells remainedin situfor months to years, with a spectrum of tissue resident memory and effector memory phenotypes.As the T-cell response intradermally is divided between targeting the gold core and the antigenic cargo, this offers a route to improving Trm formation in response to vaccines. In addition, our scRNAseq data indicate that focussing on clonally expanded skin infiltrating T-cells recruited to intradermally injected antigen is a highly efficient method to enrich and identify antigen-specific cells. This approach has the potential to be used to monitor the intradermal delivery of antigens and nanoparticles for immune modulation in humans.
Novel co-culture strategies of tumor organoids with autologous T-cells reveal clinically relevant combinations of immune-checkpoint and targeted therapies
Authors: Podaza Enrique , Capuano Jared , Assaad Majd Al , Kuo Hui-Hsuan , Markowitz Geoffrey , Irizarry Adriana , Ravichandran Hiranmayi , Ackermann Sarah , Kane Troy , Manohar Jyothi , Sigouros Michael , Moyer Jenna , Bhinder Bhavneet , Chandra Pooja , Malbari Murtaza , Boehnke Karsten , Mosquera Juan Miguel , Mittal Vivek , Sboner Andrea , Gokozan Hamza , Altorki Nasser , Elemento Olivier , Martin M. Laura 
Published: July 2023

AbstractPatient derived tumor organoids (PDTOs) have become relevant pre-clinical models for therapeutic modelling since they highly recapitulate patients’ response to treatment. Nevertheless, their value for immunotherapy modelling has not been fully explored. We developed a tumor processing protocol that enable the establishment of PDTOs and tumor infiltrating lymphocytes (TILs) isolation. By the optimization of functional assays, we compared the T-cells effector functions of matching PBMCs and TILs, demonstrating that PBMCs after co-culture and TILs after initial expansion display similar responses. In addition, the evaluation of cytokine production by fluorospot in combination with an image-based killing assay enable the screening of different immune-checkpoint inhibitors as well as its combination with target inhibitors. Our proof-of-concept functional assays showed the potential and versatility of PDTOs and T-cells co-culture systems for immunotherapy screening. The optimization of scalable functional assays downstream co-culture represents a significant step forward to increase the value of PDTOs as pre-clinical models for immunotherapeutic screens.
ESGq: Alternative Splicing events quantification across conditions based on Event Splicing Graphs
Authors: Cozzi Davide , Bonizzoni Paola , Denti Luca 
Published: July 2023

AbstractAlternative Splicing (AS) is a regulation mechanism that contributes to protein diversity and is also associated to many diseases and tumors. Alternative splicing events quantification from RNA-Seq reads is a crucial step in understanding this complex biological mechanism. However, tools for AS events detection and quantification show inconsistent results. This reduces their reliability in fully capturing and explaining alternative splicing. We introduce ESGq, a novel approach for the quantification of AS events across conditions based on read alignment against Event Splicing Graphs. By comparing ESGq to two state-of-the-art tools on real RNA-Seq data, we validate its performance and evaluate the statistical correlation of the results. ESGq is freely available athttps://github.com/AlgoLab/ESGq.