bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rab26 controls secretory granule maturation and breakdown in Drosophila
Authors: Boda Attila , Varga Luca Petra , Nagy Anikó , Szenci Győző , Csizmadia Tamás , Lőrincz Péter , Juhász Gábor 
Published: January 2023

AbstractAt the onset of Drosophila metamorphosis, plenty of secretory glue granules are released from salivary gland cells and the glue is deposited on the ventral side of the forming (pre)pupa to attach it to a dry surface. Prior to this, a poorly understood maturation process takes place during which secretory granules gradually grow via homotypic fusions, and their contents are reorganized. Here we show that the small GTPase Rab26 localizes to immature (smaller, non-acidic) glue granules and its presence prevents vesicle acidification. Rab26 mutation accelerates the maturation, acidification and release of these secretory vesicles as well as the lysosomal breakdown (crinophagy) of residual, non-released glue granules. Strikingly, loss of Mon1, an activator of the late endosomal and lysosomal fusion factor Rab7, results in Rab26 remaining associated even with the large glue granules and a concomitant defect in glue release, similar to the effects of Rab26 overexpression. Our data thus identify Rab26 as a key regulator of secretory vesicle maturation that promotes early steps (vesicle growth) and inhibits later steps (lysosomal transport, acidification, content reorganization, release, and breakdown), which is counteracted by Mon1.
Thrombotisch-thrombozytopenische Purpura – eine differenzialdiagnostische Herausforderung im Notfall
Authors: Özcan Fedai , Köhrmann Martin , Petros Sirak , Goette Andreas , Bramlage Peter , Bommer Martin , Brokmann Jörg Christian 
Published: January 2023

ZusammenfassungDas Vorliegen einer thrombotisch-thrombozytopenischen Purpura (TTP) als Kombination aus Thrombozytopenie, LDH-Erhöhung und Anämie in Kombination mit einer Organbeteiligung ist eine seltene, aber lebensbedrohliche Erkrankung, die unbehandelt mit einer extrem hohen Letalität in der Akutphase einhergeht. Wir stellen in dem vorliegenden Beitrag den Fall einer 49-jährigen Patientin vor, die mit unklaren abdominellen Beschwerden und subfebrilen Temperaturen stationär aufgenommen wurde, und leiten daraus Empfehlungen für die Notfallsituation ab. Ein erhöhtes Bewusstsein für die Erkrankung und die zielgerichtete weiterführende Diagnostik mit Bestimmung des PLASMIC-Scores bzw. der ADAMTS13-Aktivität führt ggf. direkt zur TTP, deren verzögerte Diagnostik regelhaft zu Sekundärkomplikationen führen würde.
Recent progress of metal–organic frameworks as sensors in (bio)analytical fields: towards real-world applications
Authors: Zuliani Alessio , Khiar Noureddine , Carrillo-Carrión Carolina 
Published: January 2023

AbstractThe deployment of metal–organic frameworks (MOFs) in a plethora of analytical and bioanalytical applications is a growing research area. Their unique properties such as high but tunable porosity, well-defined channels or pores, and ease of post-synthetic modification to incorporate additional functional units make them ideal candidates for sensing applications. This is possible because the interaction of analytes with a MOF often results in a change in its structure, eventually leading to a modification of the intrinsic physicochemical properties of the MOF which is then transduced into a measurable signal. The high porosity allows for the adsorption of analytes very efficiently, while the tunable pore sizes/nature and/or installation of specific recognition groups allow modulating the affinity towards different classes of compounds, which in turn lead to good sensor sensitivity and selectivity, respectively. Some figures are given to illustrate the potential of MOF-based sensors in the most relevant application fields, and future challenges and opportunities to their possible translation from academia (i.e., laboratory testing of MOF sensing properties) to industry (i.e., real-world analytical sensor devices) are critically discussed.
Graphical abstract
Impact of Fluoro‐Choline PET/CT in Reduction in Failed Parathyroid Localization in Primary Hyperparathyroidism
Authors: Aphale Rijuta , Damle Nishikant , Chumber Sunil , Khan Maroof , Khadgawat Rajesh , Dharmashaktu Yamini , Agarwal Shipra , Bal Chandrashekhar 
Published: January 2023

AbstractIntroductionAccurate localization of the pathological parathyroid gland is a prerequisite for minimally invasive surgical management of hyperparathyroidism (HPT). Poor imaging or discordance in odd situations like ectopic adenomas, parathyroid hyperplasia, syndromic HPT results in localization dilemma thus causing failed parathyroidectomy. We studied the impact of Fluoro‐Choline (FCH) PET/CT imaging in reduction in localization failure of parathyroid adenoma.Materials and methodsWe did a retrospective observational study (2018–2021) of HPT among which 97 patients underwent focused parathyroidectomy (FP). All patients had undergone ultrasound imaging and 99mTc‐sestaMIBI scan with early SPECT/CT (MIBI). When this preliminary imaging was doubtful or negative or multiple lesions were expected, FCH PET/CT was performed. We compared the localization accuracy of MIBI scan and FCH PET/CT with surgical outcomes as reference standard.ResultsMIBI scan showed overall lesion detection rate (LDR) of 88.65% in localization of pathological parathyroid gland in 97 patients. The addition of FCH PET/CT improved the overall lesion detection to 97.9%. The overall possible localization failure was reduced from 11.34 to 2.06% with the addition of FCH PET/CT (p < 0.05).Out of 97 patients of FP, 87 patients showed features of parathyroid adenoma. Single hyperplastic gland was seen in 7 patients, lipoadenoma was seen in 1 patient and 1 patient had features suggestive of parathyroiditis on histopathology. FCH PET/CT was a useful adjunct and showed significant reduction in localization failure of parathyroid adenoma.
Chronische Entzündungserkrankungen in Deutschland
Authors: Leipe Jan , Schmelz Renate , Riemekasten Gabriela , Thaçi Diamant , Henes Jörg , Schäkel Knut , Pinter Andreas , Sticherling Michael , Wegner Joanna , Fusco Stefano , Linke Miriam , Weber Valeria , Manz Karina C. , Bartz Holger , Roecken Marit , Schmidt Sandra , Hoyer Bimba F. 
Published: January 2023

Zusammenfassung
Hintergrund
Chronische Entzündungserkrankungen (engl. „immune-mediated inflammatory diseases“ [IMID]) sind mit einer erheblichen Krankheitslast verbunden. Diese ist umso ausgeprägter, sofern diese gleichzeitig bei Patienten auftreten oder z. B. weitere Komorbiditäten bestehen. Die Versorgung der IMID ist komplex und involviert diverse medizinische Fachdisziplinen.

Ziel der Arbeit
Die Beschreibung der Krankheitslast und der aktuellen Arzneimittelversorgung der Patienten mit IMID.

Material und Methoden
Die retrospektive Querschnittanalyse erfolgte unter Nutzung von Abrechnungsdaten der gesetzlichen Krankenversicherung der InGef-Datenbank. Unter 3.988.695 Versicherten wurden im Jahr 2018 anhand dokumentierter Diagnosen (ICD-10-GM) prävalente Patienten mit Psoriasis (Pso), Psoriasisarthritis (PsA), Spondyloarthritiden (SpA), rheumatoider Arthritis (RA), Morbus Crohn (MC), Colitis ulcerosa (CU) oder Kollagenosen identifiziert. Das gemeinsame Auftreten verschiedener IMID sowie weiterer Begleiterkrankungen wurde im Vergleich zur Referenzpopulation untersucht. Die medikamentöse Versorgung wurde basierend auf vordefinierten Therapieformen beschrieben.

Ergebnisse
Im Jahr 2018 wurden 188.440 Patienten mit IMID (4,7 %) identifiziert. Im Vergleich zur Referenzpopulation war die Prävalenz von depressiven Episoden und kardiovaskulären Risikoerkrankungen bei Patienten mit IMID höher. Bei MC, CU, RA, und PsA wurden DMARDs (engl. „disease-modifying antirheumatic drugs“) und klassische systemische Therapien am häufigsten eingesetzt. Bei Pso, SpA und Kollagenosen waren NSAR (nichtsteroidale Antirheumatika) die häufigsten Therapieformen oft in Kombination mit anderen Wirkstoffen.

Diskussion
Ein beträchtlicher Anteil der Patienten mit IMIDs (16,9–27,5 %) leidet an unterschiedlichen Erkrankungen des IMID-Formenkreises. Sie sind häufig von Begleiterkrankungen betroffen und bedürfen einer interdisziplinären medizinischen Versorgung.
Early radiographic osseointegration of a novel highly porous 3D-printed titanium collar for megaprostheses compared to a previous generation smooth HA-coated collar
Authors: Haider Thomas , Pagkalos Iosif , Morris Guy , Parry Michael C. , Jeys Lee M. 
Published: January 2023

Abstract
Purpose
Extracortical osseointegration at the collar-bone interface of megaprostheses is associated with improved implant stability, lower rates of stem fracture and loosening. The use of hydroxy-apatite (HA-) coated collars showed mixed results in previously published reports. A novel collar system has recently become available utilizing additive manufacturing technology to create a highly porous titanium collar with a calcium-phosphate coated surface. The aim of this study was to evaluate our early experience with this novel collar and compare it to the previously used HA-coated model.

Methods
Twenty patients who underwent megaprostheses implantation utilizing the novel collar system were case matched to 20 patients who had previously undergone a HA-coated collar. A minimum radiological follow-up of three months was available in all included patients. Osseointegration was evaluated using postoperative plain radiographs in two planes based on a previously published semi-quantitative score.

Results
Compared to the HA-coated collar the use of the novel highly porous collar was associated with a higher proportion of cases demonstrating osseointegration at the bone-collar interface (80% vs. 65%). Application of the highly porous collar led to a significantly shortened time to reach the final ongrowth score (173 ± 89 days vs. 299 ± 165 days, p < 0.05). At one year follow-up, 90% of the novel collars had reached their final osseoingration grade compared to 50% in the HA-coated collar group (p < 0.001). Radiological osseointegration was seen in 71% for highly porous collars where the indication was revision arthroplasty, compared to 27% in reported in the literature.

Conclusion
These results indicate more reliable and accelerated osseointegration at the bone-collar interface of a novel highly porous collar system compared to a previously used HA-coated collar. Further studies are warranted to confirm these findings.
A high-efficient and naked-eye visible CRISPR/Cas9 system in Arabidopsis
Authors: Kong Wenwen , Wang Mingliang , Huang Lijuan , Wu Feiyan , Tao Jinyuan , Mo Beixin , Yu Yu 
Published: January 2023

Abstract
Main conclusion
Introducing 35S-dsRED2 into the Cas9 vector which expresses naked-eye visible dsRED2 greatly facilitates the genetic screening, and the WUS promoter driving the Cas9 expression can improve editing efficiency in Arabidopsis.

Abstract
CRISPR/Cas9-dependent genome editing has been applied to generate random insertions and deletions, targeted insertions or replacements, and precise base changes for both fundamental studies in many plant species and crop improvement. To simplify the screening procedure for target gene-edited transformants, we introduced a CaMV 35S-driven dsRED2 cassette (35S-dsRED2) into the Cas9 vector to express the naked-eye visible protein dsRED2, which can be observed under white light, greatly facilitated the genetic screening and reduced labor intensity without using any instrument. In addition, the WUS promoter was used to drive the expression of Cas9, which successfully improved the target genes editing efficiency and enabled the homozygous mutagenesis of two genes in T1 generation in Arabidopsis. Considering the conserved function and expression pattern of WUS across the plant species, this dsRED2-WUS/Cas9 system could also be used in many crops.
Populational heterogeneity and partial migratory origin of the ventromedial hypothalamic nucleus: genoarchitectonic analysis in the mouse
Authors: López-González Lara , Martínez-de-la-Torre Margaret , Puelles Luis 
Published: January 2023

AbstractThe ventromedial hypothalamic nucleus (VMH) is one of the most distinctive hypothalamic tuberal structures, subject of numerous classic and modern functional studies. Commonly, the adult VMH has been divided in several portions, attending to differences in cell aggregation, cell type, connectivity, and function. Consensus VMH partitions in the literature comprise the dorsomedial (VMHdm), and ventrolateral (VMHvl) subnuclei, which are separated by an intermediate or central (VMHc) population (topographic names based on the columnar axis). However, some recent transcriptome analyses have identified a higher number of different cell types in the VMH, suggesting additional subdivisions, as well as the possibility of separate origins. We offer a topologic and genoarchitectonic developmental study of the mouse VMH complex using the prosomeric axis as a reference. We analyzed genes labeling specific VMH subpopulations, with particular focus upon the Nkx2.2 transcription factor, a marker of the alar-basal boundary territory of the prosencephalon, from where some cells seem to migrate dorsoventrally into VMH. We also identified separate neuroepithelial origins of a Nr2f1-positive subpopulation, and a new Six3-positive component, as well as subtle differences in origin of Nr5a1 positive versus Nkx2.2-positive cell populations entering dorsoventrally the VMH. Several of these migrating cell types are born in the dorsal tuberal domain and translocate ventralwards to reach the intermediate tuberal domain, where the adult VMH mass is located in the adult. This work provides a more detailed area map on the intrinsic organization of the postmigratory VMH complex, helpful for deeper functional studies of this basal hypothalamic entity.
Postnatal growth and body composition in extremely low birth weight infants fed with individually adjusted fortified human milk: a cohort study
Authors: Perrin Tania , Pradat Pierre , Larcade Julie , Masclef-Imbert Marion , Pastor-Diez Blandine , Picaud Jean-Charles 
Published: January 2023

AbstractThis cohort study aimed to evaluate the impact of an individualised nutritional care approach combining standardised fortification with adjustable fortification on postnatal growth and body composition in extremely low birth weight (ELBW) infants. We included ELBW infants admitted to a neonatal intensive care unit and still hospitalised at 35 weeks postmenstrual age (PMA). The fortification of human milk was standardised (multicomponent fortifier) between 70 mL/kg/day and full enteral feeding, and then individualised using adjustable fortification. When weight gain was below 20 g/kg/day, protein or energy was added when serum urea was below or above 3.5 mmol/L, respectively. Postnatal growth failure (PNGF) was defined as being small for gestational age at discharge and/or when the Z-score loss between birth and discharge was higher than 1. Body composition was assessed between 35 and 41 weeks of PMA. Among the 310 ELBW infants included, the gestational age of birth was 26.7 ± 1.8 weeks, and the birth weight was 800 ± 128 g. The mean Z-score difference between birth and discharge was moderately negative for the weight (−0.32), more strongly negative for length (−1.21), and almost nil for head circumference (+ 0.03). Only 27% of infants presented PNGF. At discharge, fat mass was 19.8 ± 3.6%. Multivariable analysis showed that the proportion of preterm formula received and gestational age at birth were independently associated with the percentage of fat mass. Conclusion: The individualised nutritional care approach applied herein prevented postnatal weight loss in most infants, limited length growth deficit, and supported excellent head circumference growth.

What is Known:• At least half of extremely low birth weight infants are small for gestational age at discharge and postnatal growth deficit has been associated with impaired neurocognitive and renal development.• Human milk is the main milk used in neonatology and, although fortification of human milk is a standard of care, there is no consensus regarding the optimal fortification strategy to be adopted.

What is New:• Using an approach combining standardised fortification followed by individualised adjustable fortification limited postnatal growth deficit for body weight and head circumference. Postnatal growth failure is not a fatality in extremely low birth weight infants.• Each additional gestational age week at birth resulted in a decrease in fat mass percentage at discharge, which was higher than in foetuses of the same gestational age, likely representing a necessary adaptation to extra-uterine life.
Spatio-temporal stochastic differential equations for crime incidence modeling
Authors: Calatayud Julia , Jornet Marc , Mateu Jorge 
Published: January 2023

AbstractWe propose a methodology for the quantitative fitting and forecasting of real spatio-temporal crime data, based on stochastic differential equations. The analysis is focused on the city of Valencia, Spain, for which 90247 robberies and thefts with their latitude-longitude positions are available for a span of eleven years (2010–2020) from records of the 112-emergency phone. The incidents are placed in the 26 zip codes of the city (46001–46026), and monthly time series of crime are built for each of the zip codes. Their annual-trend components are modeled by Itô diffusion, with jointly correlated noises to account for district-level relations. In practice, this study may help simulate spatio-temporal situations and identify risky areas and periods from present and past data.
On the Controllability of Entropy Solutions of Scalar Conservation Laws at a Junction via Lyapunov Methods
Authors: De Nitti Nicola , Zuazua Enrique 
Published: January 2023

AbstractIn this note, we prove a controllability result for entropy solutions of scalar conservation laws on a star-shaped graph. Using a Lyapunov-type approach, we show that, under a monotonicity assumption on the flux, if u and v are two entropy solutions corresponding to different initial data and same in-flux boundary data (at the exterior nodes of the star-shaped graph), then u ≡ v for a sufficiently large time. In order words, we can drive u to the target profile v in a sufficiently large control time by inputting the trace of v at the exterior nodes as in-flux boundary data for u. This result can also be shown to hold on tree-shaped networks by an inductive argument. We illustrate the result with some numerical simulations.
Dynamic Mechanisms Associated with the Structure and Evolution of Roll Vortices and Coherent Turbulence in the Hurricane Boundary Layer: A Large Eddy Simulation During the Landfall of Hurricane Harvey
Authors: Li Xin , Pu Zhaoxia 
Published: January 2023

AbstractRoll vortices are a series of large-scale turbulent eddies that nearly align with the mean wind direction and prevail in the hurricane boundary layer. In this study, the one-way nested WRF-LES model simulation results from Li et al. (J Atmos Sci 78(6):1847–1867, https://doi.org/10.1175/JAS-D-20-0270.1, 2021) are used to examine the structure and generation mechanism of roll vortices and associated coherent turbulence in the hurricane boundary layer during the landfall of Hurricane Harvey from 00 UTC 25 to 18 UTC 27 August 2017. Results indicate that roll vortices prevail in the hurricane boundary layer. The intense roll vortices and associated large turbulent eddies above them (at a height of ~ 200 to 3000 m) accumulate within a hurricane radius of 20–40 km. Their intensity is proportional to hurricane intensity during the simulation period. Before and during hurricane landfall, strong inflow convergence leads to horizontal advection of roll vortices throughout the entire hurricane boundary layer. Combined with the strong wind shear, the strongest roll vortices and associated large turbulent eddies are generated near the eyewall with suitable thermodynamic (Richardson number at around − 0.2 to 0.2) and dynamic conditions (strong negative inflow wind shear). After landfall, the decayed inflow weakens the inflow convergence and quickly reduces the strong roll vortices and associated large turbulent eddies. Diagnosis of vertical turbulent kinetic energy indicates that atmospheric pressure perturbation, caused by horizontal convergence, transfers the horizontal component of turbulence to the vertical component with a mean wavelength of about 1 km. The buoyancy term is weak and negative, and the large turbulent eddies are suppressed.
Flame-retardant finishing of cotton fabrics using DOPO functionalized alkoxy- and amido alkoxysilane
Authors: Ali Wael , Zilke Olga , Danielsiek Dominic , Salma Alaa , Assfour Bassem , Shabani Valbone , Caglar Seden , Phan Hung Minh , Kamps Leonie , Wallmeier Ruth , Feng Ying , Textor Torsten , Gutmann Jochen S. , Mayer-Gall Thomas 
Published: January 2023

AbstractIn the present study, DOPO-based alkoxysilane (DOPO-ETES) and amido alkoxysilane (DOPO-AmdPTES) were synthesized by one-step and without by-products as halogen-free flame retardants. The flame retardants were applied on cotton fabric utilizing sol–gel method and pad-dry-cure finishing process. The flame retardancy, the thermal stability and the combustion ehaviour of treated cotton were evaluated by surface and bottom edge ignition flame test (according to EN ISO 15025), thermogravimetric analysis (TGA) and micro-scale combustion calorimeter (MCC). Unlike CO/DOPO-ETES sample, cotton treated with DOPO-AmdPTES nanosols exhibits self-extinguishing ehaviour with high char residue, an improvement of the LOI value and a significant reduction of the PHRR, HRC and THR compared to pristine cotton. Cotton finished with DOPO-AmdPTES reveals a semi-durability after ten laundering cycles keeping the flame-retardant properties unchanged. According to the results obtained from TGA-FTIR, Py-GC/MS and XPS, the major activity of flame retardant occurs in the condensed phase via catalytic induced char formation as physical barrier along with the activity in the gas phase derived mainly from the dilution effect. The early degradation of CO/DOPO-AmdPTES compared to CO/DOPO-ETES, triggered by the cleavage of the weak bond between P and C=O, as the DFT study indicated, provides the beneficial effect of this flame retardant on the fire resistance of cellulose.
Graphical abstract
Behavioural and Welfare Analysis of an Intermediary in Biodiversity Offset Markets
Authors: Kangas Johanna , Ollikainen Markku 
Published: January 2023

AbstractThis paper provides a behavioural and welfare analysis of an intermediary in biodiversity offset markets. These markets are characterised by high information requirements and transaction costs, threatening economic efficiency and even biodiversity outcomes. Specialised intermediaries facilitate trading by providing information and brokering services. By buying, holding and selling offset credits from storage, the intermediary can decrease both financial and ecological risks in the market. As a drawback, the intermediary may exploit market power upstream or downstream due to ecological features of the offset market. Intermediaries decrease the trading parties’ transaction costs by offering specialised information, reduce uncertainty, and decrease the costs of offsetting by increasing liquidity in the market and offering certain offset credits. When the intermediary has market power, selling and buying prices deviate from the competitive equilibrium. This welfare loss may be lower than the loss from transaction costs and trade ratios in decentralised trade, even in the case of the intermediary having both monopoly and monopsony power. The intermediary is the most useful when trade ratios are high and when the intermediary stores mature credits, which eliminates ecological uncertainty and thereby offers cost savings for developers, and may result in a higher level of biodiversity.