bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeted rRNA depletion enables efficient mRNA sequencing in diverse bacterial species and complex co-cultures
Authors: Heom Kellie A. , Wangsanuwat Chatarin , Butkovich Lazarina V. , Tam Scott C. , Rowe Annette R. , O’Malley Michelle A. , Dey Siddharth S. 
Published: May 2023

ABSTRACTBacterial mRNA sequencing is inefficient due to the abundance of ribosomal RNA that is challenging to deplete. While commercial kits target rRNA from common bacterial species, they are frequently inefficient when applied to divergent species, including those from environmental isolates. Similarly, other methods typically employ large probe sets that tile the entire length of rRNAs; however, such approaches are infeasible when applied to many species. Therefore, we present EMBR-seq+, which requires fewer than ten oligonucleotides per rRNA by combining rRNA blocking primers with RNase H-mediated depletion to achieve rRNA removal efficiencies of up to 99% in diverse bacterial species. Further, in more complex microbial co-cultures betweenF. succinogenesstrain UWB7 and anerobic fungi, EMBR-seq+ depleted both bacterial and fungal rRNA, with a 4-fold improvement in bacterial rRNA depletion compared to a commercial kit, thereby demonstrating that the method can be applied to non-model microbial mixtures. Notably, for microbes with unknown rRNA sequences, EMBR-seq+ enables rapid iterations in probe design without requiring to start experiments from total RNA. Finally, efficient depletion of rRNA enabled systematic quantification of the reprogramming of the bacterial transcriptome when cultured in the presence of the anerobic fungiAnaeromyces robustusorCaecomyces churrovis.We observed thatF. succinogenesstrain UWB7 downregulated several lignocellulose-degrading carbohydrate-active enzymes in the presence of anerobic gut fungi, suggesting close interactions between two cellulolytic species that specialize in different aspects of biomass breakdown. Thus, EMBR-seq+ enables efficient, cost-effective and rapid quantification of the transcriptome to gain insights into non-model microbial systems.
PLGA-based dual-loaded nanoformulation of DIM and TMZ - An advanced clinical strategy for brain cancer treatment in a combinatorial approach
Authors: Sarkar Sibani , Kumar Sunny , Saha Gouranga , Basu Malini , Ghosh Mrinal K. 
Published: May 2023

AbstractGlioblastoma multiforme (GBM) is a highly aggressive form of primary brain tumor in adults, which unfortunately has an abysmal prognosis and poor survival rates. Even though several FDA-approved multimodal treatments for targeting GBM are available, the effectiveness in most patients are not satisfactory. The reason behind this poor success rate is mainly attributed to insufficient drug distribution to the tumor site across the blood-brain barrier (BBB) and induction of resistance for single-drug based therapies. Chemotherapy with Temozolomide (TMZ) having a median overall survival of around 12-15 months, envisages the urgent necessity for more effective treatment strategies. Based upon these facts, in this study, we have developed a novel approach for repurposing TMZ along with inhibition of EGFR, which overexpressed in GBM, to achieve our goal. PLGA-based nanoencapsulation of both TMZ and 3,3’-diindoyl methane (DIM), an EGFR inhibitor, in a combinatorial approach enhances the delivery of them together. Their synergistic mode of actions, significantly enhances the cytotoxic effect of TMZin vitroandin vivo. Moreover, the dual-loaded nanoformulation works more efficiently than their individually packed nanoparticles on DNA damage and apoptosis, resulting in a several-fold reduction in tumor burden, systemic drug toxicity, and increased survival. These findings suggest the preclinical potential of this new treatment strategy.
Structural and functional characterization of the NF-κB-targeting toxin AIP56 fromPhotobacterium damselaesubsp.piscicidareveals a novel mechanism for membrane interaction and translocation
Authors: Lisboa Johnny , Pereira Cassilda , Pinto Rute D. , Rodrigues Inês S. , Pereira Liliana M. G. , Pinheiro Bruno , Oliveira Pedro , Pereira Pedro J. B. , Azevedo Jorge E. , Durand Dominique , Benz Roland , Vale Ana do , Santos Nuno M. S. dos 
Published: May 2023

ABSTRACTBacterial AB toxins are secreted virulence factors that are internalized by target cells through receptor-mediated endocytosis, translocating their enzymatic domain to the cytosol from endosomes (short-trip) or the endoplasmic reticulum (long-trip). To accomplish this, they evolved a multidomain structure organized into either a single polypeptide chain or non-covalently associated polypeptide chains. The prototypical short-trip single-chain toxin is characterized by a receptor-binding domain that confers cellular specificity and a translocation domain responsible for pore formation whereby the catalytic domain translocates to the cytosol in an endosomal acidification-dependent way.In this work, the resolution of the three-dimensional structure of AIP56 showed that instead of a two-domain organization, as previously predicted, AIP56 has a three-domain organization, with a NleC-like catalytic domain associated with a small middle domain that contains the linker-peptide, followed by the receptor-binding domain. In contrast to prototypical single-chain AB toxins, AIP56 does not comprise a typical structurally complex translocation domain; instead, the elements involved in translocation are scattered across its domains. Thus, the catalytic domain contains a helical hairpin that serves as a molecular switch for triggering the conformational changes necessary for membrane insertion only with endosomal acidification, whereas the middle and receptor-binding domains are required for pore formation. Considering the increasing number of putative AIP56 homologous toxins in databases, the data presented here have implications far beyond the AIP56 intoxication process and the virulence ofPhdp.
Linking learning goal orientation to learning from error: the mediating role of motivation to learn and metacognition
Authors: Lauzier Martin , Bilodeau Clarke Annabelle 
Published: May 2023

Purpose
Errors are increasingly recognized as beneficial to the learning process and are more frequently integrated into training curriculums. Despite this growing interest, the work carried out so far offers little evidence highlighting the psychological qualities implicit in learning from error. By focussing on the role of specific trainee’s attributes [i.e. learning goal orientation (LGO) motivation to learn and metacognition], this study aims to better understand the reasons why some trainees benefit more (than others) from being confronted with errors during training.

Design/methodology/approach
A total of 142 trainees took part in this study by participating in a training on interviewing techniques that also exposed them to various committable errors, and by completing questionnaires at two different times (i.e. before and after training).

Findings
Results of bootstrap regression analysis highlights three main findings: LGO is positively linked to learning from errors; a significant portion of the link between LGO and learning from error is explained by motivation to learn and metacognition; and these effects are presented in the form of a double-mediated model which suggests two different explanatory pathways (i.e. motivational and cognitive).

Originality/value
To the best of the authors’ knowledge, this study is among the first to offer insight on the psychological attributes influencing learning from errors and to bring forward the role of two underlying mechanism that are linked to this specific type of learning. It also invites researchers and practitioners to reflect on the best ways to make use of errors in training and promote the value of personal attributes on trainees’ learning experience.
The transition from traditional to digital: factors that propel Generation Z’s adoption of online learning
Authors: Bagdi Himanshu , Bulsara Hemantkumar P. , Sankar Deepthi , Sharma Latika 
Published: May 2023

Purpose This study aims to identify factors affecting Generation Z (Gen-Z)’s online learning (OL) behavioural intention (BI) using the extended technology acceptance model by investigating gender differences in the BI of Indian students to adopt OL.Design/methodology/approach The proposed model was tested on 284 students from a higher education institution (HEI) in Gujarat, India, using the partial least square–structural equation modelling. Further, the subgroup analysis was conducted using Statistical Package for Social Sciences (SPSS) to determine male and female OL adoption variances.Findings Based on the research findings, all the constructs positively influence the BI of Gen-Z to adopt OL, except the influence of information technology infrastructure (INF) on perceived usefulness (PU). However, no substantial difference was observed in the BI adoption of OL amongst males and females. Also, when gender was kept as a moderator between computer self-efficacy (CSE) and PU, and INF and perceived ease of use (PEU), no substantial difference was observed in the OL of Gen-Z.Research limitations/implications The study will benefit HEIs and associated government departments from a deeper understanding of Gen-Z’s BI to speed up deployment and consider key elements essential while considering OL.Originality/value This article found major determinants of Gen-Z’s BI to adopt the OL in HEI. To the best of the author’s understanding, this is amongst the few studies to explore these ideas in the context of India. The uniqueness of the topic has been brought to light by the fact that “gender” has been employed as a moderator between attitude and BI, INF and PEU, and CSE and PU.
Fatal rupture of hepatic adenomatosis: Autopsy case and review of the literature
Authors: Ben Abderrahim Sarra , Chérif Khouloud , Nfikha Zeineb , Gharsallaoui Sarra , El Aini Imen , Jedidi Maher , Mokni Moncef , Ben Dhiab Mohamed 
Published: May 2023

AbstractHepatic adenomatosis is a rare disease consisting of multiple adenomas in otherwise‐normal liver parenchyma. Though the discovery of this entity goes back several years, its diagnosis is still challenging in terms of its definition and pathophysiology. Clinically, patients may be completely asymptomatic and the diagnosis is only made incidentally through imaging tests. The discovery could be made when complications occur such as intraperitoneal hemorrhage with hypovolemic shock due to the rupture of an adenoma. We report a fatal case of a ruptured adenoma in a case of hepatic adenomatosis discovered at autopsy. In order to achieve a better view of this disease, we conducted a literature review on this subject describing the pathogenesis, manifestations, and autopsy contribution to addressing this entity.
Damage accrual in Behçet disease: Behçet's Syndrome Overall Damage Index (BODI) versus Behçet's Disease Damage Index (BDI)
Authors: Hassan Rofaida A. , Abdelhady Enas I. , Rabie Mona , Kamal Doaa E. 
Published: May 2023

AbstractObjectivesTo assess the validity of Behçet's Syndrome Overall Damage Index (BODI) and Behçet's Disease Damage Index (BDI) as tools for the detection of damage accrual in Behçet's patients compared to Vasculitis Damage Index (VDI). Also, to evaluate the correlation and the interclass correlation among the 3 indices to find out their consistency.MethodA prospective cohort study was carried out on 102 adult Behçet's disease (BD) patients who were diagnosed according to the International Study Group criteria for BD. Disease severity and organ damage were assessed for each patient by VDI, BDI and BODI at baseline and 1‐year follow‐up visits. Damage accrual for each index was defined when there was an increase of at least 1 point (∆ ≥ 1) among the baseline and the follow‐up visits.ResultsCorrelations among the 3 indices were significant, with (r = 0.835, P < 0.001) between VDI and BODI, (r = 0.835, P < 0.001) between VDI and BDI, and (r = 0.844, P < 0.001) between BODI and BDI scores. A highly significant positive correlation existed between the 3 indices and age and disease duration. In contrast, the correlation with the BD Current Activity Form was non‐significant, which indicates good discriminative validity of the 3 indices. Neuropsychiatric and ocular systems showed a strong interclass correlation among the 3 indices. Regarding detecting damage accrual, BDI was more sensitive than BODI and showed more agreement with VDI.ConclusionBD damage indices, VDI, BODI and BDI, had good convergent and discriminative validity for the assessment of BD damage. BDI had more sensitivity than BODI to the detection of damage accrual.
Maternal near miss at Kathmandu Medical College: An analysis of severe maternal morbidity at a Nepalese tertiary care facility
Authors: Manandhar Rosina , Adhikari Ashmita , Manandhar Naresh , Jayaratnam Skandarupan 
Published: May 2023

AbstractBackgroundThe World Health Organization (WHO) ‘near miss’ tool has been extensively used to audit maternal morbidity in low‐ and middle‐income countries. Analysis of the cases of ‘near miss’ enables a better understanding of the associated factors, identifies deficiencies in the provision of maternity services and lays a foundation for better preventive measures in the future.AimsTo understand the epidemiology, aetiology and determine the aspects of preventability of maternal ‘near miss’ (MNM) at Kathmandu Medical College.Materials and MethodsA prospective audit of MNM and maternal deaths (MD) was undertaken at Kathmandu Medical College over a period of 12 months. The cases were identified using WHO ‘near miss’ criteria and areas of preventability in the provision of care determined using the modified Geller's criteria.ResultsThe total number of deliveries and live births in the study period were 2747 and 2698 respectively. A total of 34 ‘near misses’ and two MDs were identified. The common direct aetiologies of MNM and MDs identified were obstetric haemorrhage followed by hypertensive disorders with one‐third of cases being of indirect aetiology. Fifty‐five percent of cases had some aspects of provider‐ or system‐related preventability with the leading delays being lack of diagnosis and recognition of high‐risk status among patients and lack of interdepartmental communication.ConclusionThe WHO near miss rate at Kathmandu Medical College was 12.5/100 live births. Significant aspects of preventability, especially at the level of the provider, were noted among cases of MNM and MDs.
Institutional support for new work roles: The case of care coordinators in the United States and England
Authors: Krachler Nick 
Published: May 2023

AbstractDrawing on comparative employment relations literature, this article explores how employment relations (ER) institutions support the ‘care coordinator’, a new role tasked with aiding the exchange of information between health and social services in the United States and the UK. Findings show that in both countries, multi‐employer collective bargaining facilitated this role by providing good working conditions and a stable work environment; additionally, the new role performed better in England due to the broader scope of bargaining and supportive management practices. The article advances a comparative institutional perspective on the creation of new tasks focused on sub‐national (sectoral and regional) ER.
Advances and applications of fluids biomarkers in diagnosis and therapeutic targets of Alzheimer's disease
Authors: Xu Yanan , Jiang Hailun , Zhu Bin , Cao Mingnan , Feng Tao , Sun Zhongshi , Du Guanhua , Zhao Zhigang 
Published: May 2023

AbstractAimsAlzheimer's disease (AD) is a neurodegenerative disease with challenging early diagnosis and effective treatments due to its complex pathogenesis. AD patients are often diagnosed after the appearance of the typical symptoms, thereby delaying the best opportunity for effective measures. Biomarkers could be the key to resolving the challenge. This review aims to provide an overview of application and potential value of AD biomarkers in fluids, including cerebrospinal fluid, blood, and saliva, in diagnosis and treatment.MethodsA comprehensive search of the relevant literature was conducted to summarize potential biomarkers for AD in fluids. The paper further explored the biomarkers' utility in disease diagnosis and drug target development.ResultsResearch on biomarkers mainly focused on amyloid‐β (Aβ) plaques, Tau protein abnormal phosphorylation, axon damage, synaptic dysfunction, inflammation, and related hypotheses associated with AD mechanisms. Aβ42, total Tau (t‐Tau), and phosphorylated Tau (p‐Tau), have been endorsed for their diagnostic and predictive capability. However, other biomarkers remain controversial. Drugs targeting Aβ have shown some efficacy and those that target BACE1 and Tau are still undergoing development.ConclusionFluid biomarkers hold considerable potential in the diagnosis and drug development of AD. However, improvements in sensitivity and specificity, and approaches for managing sample impurities, need to be addressed for better diagnosis.
Progress developing the concept of other effective area‐based conservation measures
Authors: Cook Carly N. 
Published: May 2023

AbstractIn 2010, the introduction of other effective area‐based conservation measures (OECMs) into international policy caused a paradigm shift in area‐based conservation, which included consideration of areas outside formal protected areas and places where biodiversity conservation may not be a management objective for the site. Despite the importance of this shift for global conservation, conservation science and policy have been slow to engage with the concept of OECMs. As the world moves toward protecting 30% of the Earth by 2030, it is imperative to develop evidence‐based guidance for how to identify effective conservation measures, especially tools to help evaluate and monitor the biodiversity outcomes associated with potential OECMs. To understand the current progress in developing the concept of OECMs, I evaluated the peer‐reviewed literature to consolidate and synthesize current knowledge. I conducted a thematic analysis of papers to identify the types of challenges and opportunities being discussed and lessons from studies evaluating the effectiveness of OECMs. Only 105 studies mentioned OECMs, and those that did rarely move beyond superficial mention of OECMs as part of area‐based conservation. Around one‐half of studies listed potential risks or benefits of OECMs but none provided evidence these issues have materialized. Twenty‐three studies attempted to identify potential OECMs, although specific case studies were rare. The 7 studies that evaluated existing OECMs were highly critical of how they had been implemented to date. Studies that evaluated conservation outcomes were extremely rare, and suggested effectiveness must be judged on a case‐by‐case basis. The current literature not only leaves many gaps in the science required to operationalize the concept of OECMs, but also often raises additional questions that need to be addressed. If these gaps are not filled by robust science, the promised benefits for biodiversity from OECMs may never be realized.
Hidden demographic impacts of fishing and environmental drivers of fecundity in a sea turtle population
Authors: Roast Michael J. , Martins Samir , Fernández‐Peralta Lourdes , Báez José Carlos , Diame Ahmed , March David , Ouled‐Cheikh Jazel , Marco Adolfo , González‐Solís Jacob , Cardona Luis 
Published: May 2023

AbstractFisheries bycatch is a critical threat to sea turtle populations worldwide, particularly because turtles are vulnerable to multiple gear types. The Canary Current is an intensely fished region, yet there has been no demographic assessment integrating bycatch and population management information of the globally significant Cabo Verde loggerhead turtle (Caretta caretta) population. Using Boa Vista island (Eastern Cabo Verde) subpopulation data from capture–recapture and nest monitoring (2013–2019), we evaluated population viability and estimated regional bycatch rates (2016–2020) in longline, trawl, purse‐seine, and artisanal fisheries. We further evaluated current nesting trends in the context of bycatch estimates, existing hatchery conservation measures, and environmental (net primary productivity) variability in turtle foraging grounds. We projected that current bycatch mortality rates would lead to the near extinction of the Boa Vista subpopulation. Bycatch reduction in longline fisheries and all fisheries combined would increase finite population growth rate by 1.76% and 1.95%, respectively. Hatchery conservation increased hatchling production and reduced extinction risk, but alone it could not achieve population growth. Short‐term increases in nest counts (2013–2021), putatively driven by temporary increases in net primary productivity, may be masking ongoing long‐term population declines. When fecundity was linked to net primary productivity, our hindcast models simultaneously predicted these opposing long‐term and short‐term trends. Consequently, our results showed conservation management must diversify from land‐based management. The masking effect we found has broad‐reaching implications for monitoring sea turtle populations worldwide, demonstrating the importance of directly estimating adult survival and that nest counts might inadequately reflect underlying population trends.
Navigating plurilingual learning: An inclusive education approach
Authors: Smythe Fiona 
Published: May 2023

AbstractLanguage experiences and linguistic knowledge of teenage migrants can be valuable resources for their continued language learning. Yet, home languages and existing plurilingual skills are often framed as obstacles to learning that tag young migrants as at risk of school failure in the context of monolingual education systems. This article draws on a comparative education study carried out in Aotearoa New Zealand and France in 2017–2019, observing young migrants' use of their languages in classroom learning. A total of 42 secondary students from a mix of asylum‐seeker, refugee, and migrant backgrounds participated, originating from 22 different countries and speaking 24 different languages. All of the students were in their first year of schooling in New Zealand or France at the time of the study. Findings draw on observations of how students made use of their existing knowledge of languages to (1) access learning in the language‐of‐schooling, (2) learn interactively through their home languages, and (3) navigate unfamiliar styles of teaching and learning, thus engaging with the unknown and developing behaviours for successful learning. Patterns across the cohort show that young migrants are adept at drawing on their existing language resources in learning. This suggests a re‐framing ofsuccess at schoolas not only outcomes, but also as defined by effective learning processes that young migrants put in place. As a result, this study suggests ways that teachers can work inclusively with young migrants through their languages, to support processes of learning that could lead to better educational outcomes.