bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Presence of Antibody to Chikungunya and Other Serologically Related Viruses in the Sera of Sub-Human Primate Imports to the United States
Authors: Harrison V. R. , Marshall J. D. , Guilloud N. B. 
Published: January 2023

Summary
Sera from 136 sub-human primates representing six genera were tested for the presence of antibody to Chikungunya, Semliki Forest Kumba strain, Onyong-nyong strain MP 87 and Mayaro strain B viruses. Twenty-six per cent of the sera tested were positive for N antibody to one or more of these agents. The extensive serologic overlap occurring between these viruses precluded any definitive interpretation of the results as to which virus may have been the specific infecting agent. Thirteen per cent of the sera tested were positive for CF and HI antibody to CHIK virus. In every instance a positive CF and HI response was associated with the presence of N antibody. Attempts at viral isolation from those sera of recent imports showing CF and HI antibody were unsuccessful.
That extreme care should be exercised in the selection of lower primates for purposes of studying exotic viral agents is clearly indicated by the data presented. Although no viremia was found in the animals tested, adherence to a rigid program of housing and protective screening is essential if a serious transmission hazard is to be minimized, particularly in areas of high arthropod activity.
Immune Hemolysis: An Inter-Species Study
Authors: Linscott William D. 
Published: January 2023

Summary
Chicken hemoglobin was found to absorb maximally at slightly different wavelengths than hemoglobins from the sheep, mouse, rat, guinea pig, human and rabbit. Peak absorption in the 414 mµ region of the spectrum was from 7.7 to 8.8 times as great as absorption in the 541 mµ region.
Antisera against sheep E were found to be uniquely low in the ratio of hemagglutinins to hemolytically active antibodies, particularly in early antisera. Guinea pig complement (C′) was approximately 25 times as active hemolytically against sheep erythrocytes (E) as rabbit C′, but only three to six times as active against E from several other species.
In a pooled guinea pig antiserum against rabbit E, nearly all the hemolytic activity was present in the “7S” antibody fraction. While agglutinating activity was present in both “7S” and “19S” fractions, the macroglobulin fraction showed very little ability to fix C′ in the presence of rabbit E.
When used with rabbit C′ many antisera showed a biphasic sensitization curve, with decreasing lysis followed by a sharp increase in lysis, as the amount of antiserum was progressively reduced. In addition, many antisera produced maximal sensitization for lysis by rabbit C′ at concentrations significantly lower than required for maximal sensitization for lysis by guinea pig C′. Both of these effects were seen with “7S” and “19S” antibody fractions as well as with whole antiserum.
Certain rabbit antisera against sheep E produced more hemolysis with rabbit C′ at 37° than at 30°C. Many such antisera and fractions which were tested showed a narrow range of concentrations within which lysis was greater at 30°, but at concentrations higher than this lysis was almost invariably greater at 37°. Other antisera produced more lysis at 30° than at 37°, at all concentrations which were tested.
BETTER OUTCOME OF HIGH-DOSE CEFTAZIDIME IN HEMATO – ONCOLOGICAL PATIENTS WITH INFECTIONS CAUSED BY EXTENSIVELY DRUG-RESISTANT PSEUDOMONAS AERUGINOSA
Authors: Zavrelova Alzbeta , Radocha Jakub , Paterova Pavla , Zak Pavel , Visek Benjamin , Sima Martin 
Published: January 2023

Background: P. aeruginosa sepsis in immunocompromised patients is serious complication of cancer treatment, especially in case of XDR pathogen.
The purpose of the study is to evaluate the efficacy of high-dose ceftazidime in treatment of XDR P. aeruginosa infection and to compare it with the conventionally treated cohort in hemato-oncological patients.
Methods: We identified 27 patients with XDR P. aeruginosa infection during the 2008-2018 period, 16 patients served as a conventionally treated cohort with antipseudomonal beta-lactam antibiotic in standard dose (cohort A), and 11 patients were treated with high-dose ceftazidime (cohort B). Most of the patients were neutropenic and under active treatment for their cancer in both cohorts.
Results: Mortality and related mortality were statistically significantly better for cohort B compared to cohort A, it was 18.2% and 9.1% for cohort B and 68.8% and 68.8% for cohort A, respectively. More patients in cohort A needed mechanical ventilation and renal replacement therapy, 75% and 50% for cohort A and 27.3% and 9.9% for cohort B, respectively. It corresponded well with the worst SOFA in cohort A in comparison to cohort B, 16 versus 7 respectively. Reversible neurotoxicity was seen only in two patients in cohort B.
Conclusion: Ceftazidime in high doses is a very potent ATB for the treatment of XDR P. aeruginosa infections in neutropenic cancer with acceptable toxicity.
ADVERSE PREGNANCY OUTCOMES IN SICKLE CELL TRAIT: A PROSPECTIVE COHORT STUDY EVALUATING CLINICAL AND HAEMATOLOGICAL PARAMETERS IN POSTPARTUM MOTHERS AND NEW-BORNS.
Authors: Al-Kindi R. Salam 
Published: January 2023

Abstract – Sickle cell trait (SCT) is a condition caused by the inheritance of a single allele of the abnormal haemoglobin beta gene, HbS. Carriers of SCT are generally asymptomatic, and they do not manifest the haematological and clinical abnormalities of sickle cell anaemia (SCA). However, there is evidence that they display some of the symptoms in stressful situations. Pregnancy is a stressful physiological event, and it is not clear if SCT adversely affects pregnancy outcomes, particularly those from the developing countries, who regularly suffer from nutritional insufficiency.
Objective – This study aims to investigate pregnancy outcomes in Sudanese women with SCT.
Subjects and methods – Pregnant women with (HbAS, n=34) and without (HbAA, n=60) SCT were recruited during their first trimester by El Obeid Hospital, Kordofan, Western Sudan. Ethical approval from the Faculty of Medicine, University of Khartoum, and informed consent from the participants were obtained. Detailed anthropometric, haematological, clinical, obstetric and birth outcome data were documented. Blood samples were collected at enrolment and delivery.
Results – At enrolment, the first trimester, the SCT group did not manifest SCA symptoms, and there was no difference in any of the haematological parameters between the SCT and control groups. At delivery, the women with SCT compared with the control group had lower levels of haemoglobin (Hb, p=0.000), packed cell volume (PCV, p=0.000), mean corpuscular haemoglobin (MCH, p=0.002) and neutrophil counts (p=0.045) and higher mean corpuscular volume (MCV, p=0.000) and platelet counts (p=0.000). Similarly, at delivery, the babies of SCT women had lower birth weight (p=0.000), lower Hb (p=0.045), PCV (p=0.000), MCH (p=0.000) and higher neutrophil (p=0.004) and platelet counts (p=0.000) than the babies of the healthy control group. Additionally, there were more miscarriages, stillbirths and admissions to the Special Care Baby Unit (SCBU) in the SCT group.
Conclusions – The study revealed that SCT is associated with adverse pregnancy outcomes, including maternal and neonatal anaemia, low birth weight and increased risk of still birth, miscarriage and admission to SCBU. Therefore, pregnant women with SCT should be regarded as a high-risk group, and given pre-conceptual advice and multidisciplinary antenatal and postnatal care.
DOWNREGULATION OF STEAROYL-COA DESATURASE 1 (SCD-1) PROMOTES RESISTANCE TO IMATINIB IN CHRONIC MYELOID LEUKEMIA
Authors: Altinok Gunes Buket , Hekmatshoar Yalda , Ozkan Tulin , Bozkurt Sureyya , Aydos O.Sena , Buyukasik Yahya , Aladag Elifcan , Sunguroglu Asuman 
Published: January 2023

Chronic myeloid leukemia (CML) is a malignant hematopoietic stem cell disease resulting in the fusion of BCR and ABL genes and characterized by the presence of the reciprocal translocation t(9;22)(q34;q11). BCR-ABL, a product of the BCR-ABL fusion gene, is a structurally active tyrosine kinase and plays an important role in CML disease pathogenesis. Imatinib mesylate (IMA) is a strong and selective BCR-ABL tyrosine kinase inhibitor. Although IMA therapy is an effective treatment, patients may develop resistance to IMA therapy over time. This study investigated the possible genetic resistance mechanisms in patients developing resistance to IMA. We did DNA sequencing in order to detect BCR-ABL mutations, which are responsible for IMA resistance. Moreover, we analyzed the mRNA expression levels of genes responsible for apoptosis, such as BCL-2, P53, and other genes (SCD-1, PTEN). In a group of CML patients resistant to IMA, when compared with IMA-sensitive CML patients, a decrease in SCD-1 gene expression levels and an increase in BCL-2 gene expression levels was observed. In this case, the SCD-1 gene was thought to act as a tumor suppressor. The present study aimed to investigate the mechanisms involved in IMA resistance in CML patients and determine new targets that can be beneficial in choosing the effective treatment. Finally, the study suggests that the SCD-1 and BCL-2 genes may be mechanisms responsible for resistance.
Keywords CML; Imatinib resistance; BCR-ABL mutations; SCD-1
Mucoceles of Paranasal Sinuses
Authors: Swain Santosh Kumar , Panda Swaha 
Published: January 2023

Background:
Mucoceles of paranasal sinuses are benign expansile mucus-filled cystic lesions due to chronic blockage of the sinus ostia. Due to their expansile growth, may affect nearby vital structures and lead to complications and morbidity.

Objective:
This study aims to evaluate the details of the clinical profile and management of paranasal sinus mucocele.

Materials and Methods:
This is a retrospective descriptive study of 54 patients with paranasal sinuses mucoceles that were managed with surgical interventions.

Results:
The age range of the study participants was between 12 years to 54 years. The mean age of the study patients was 28.61 years. Out of 54 patients, 34 (62.96%) were males and 20 (37.03%) females with a male-to-female ratio of 1.7:1. The frontal sinus (35.18%) was the most common sinus involved with mucocele followed by frontoethmoidal sinus (24.07%), ethmoid sinus, maxillary sinus (14.81%), and sphenoid sinus (9.25%). In this study, the headache was the most common symptom.

Conclusion:
Paranasal sinus mucocele has diverse clinical presentations. Allergic rhinitis, sinusitis, and radiation to the head-and-neck region are important predisposing factors for causing paranasal sinus mucocele. Orbital symptoms are indicators for prompt surgical intervention. Endoscopic sinus surgery is an effective and safe treatment option for paranasal sinus mucocele.
Benign Paroxysmal Positional Vertigo in Pregnancy
Authors: Swain Santosh Kumar , Vivek S. A. 
Published: January 2023

Background:
Benign paroxysmal positional vertigo (BPPV) is a common clinical condition characterized by sudden, brief paroxysmal attacks of rotation vertigo occurred by changing the head position. BPPV is the most common peripheral vestibular pathology and is found frequently in females. There are very limited data on the association between BPPV and pregnancy.

Objective:
The objective of this study was to study the BPPV during pregnancy along with details of clinical manifestations and management.

Patients and Methods:
This is a cross-sectional study where pregnant women with BPPV were enrolled. The study was conducted between April 2020 and May 2022. Detailed neurological examinations were done at the vertigo clinic.

Results:
There were seven cases of BPPV associated with pregnancy in this study. The most common symptom was rotatory vertigo. All were diagnosed with the Dix–Hallpike test. All underwent Epley maneuver and symptoms resolved. Out of seven cases, two showed low 25-hydroxyvitamin D levels.

Conclusion:
Vertigo is a morbid symptom during pregnancy that affects both pregnant women and fetal status. To improve the quality of life during gestation, clinicians should consider this BPPV as an important clinical entity in their differential diagnosis of vertigo.
The Impact of Media Supplement on the Viability, Proliferation, and Differentiation Potential of Bone Marrow-Derived Mesenchymal Stem Cells
Authors: Khamees Nibras Hatim 
Published: January 2023

Background:
Bone marrow-derived mesenchymal stem cells (bmMSCs) are one of the most promising therapeutic methods in modern medicine. These cells are grown in tissue culture media, commonly supplemented with fetal bovine serum (FBS), but such supplement carries a number of drawbacks, such as immunological reaction and composition variation. Another supplement known as insulin-transferrin-selenium could act as serum replacement and help enhance the experimental results and therapeutic value of MSCs.

Objective:
The objective was to investigate the effect of tissue culture supplement in regard to bone marrow MSCs viability, proliferation, and differentiation potential.

Materials and Methods:
Human bmMSCs were grown in tissue culture plates at seeding density of 5 × 103 cells/cm2. The cells were divided into two groups, 10% FBS supplement or insulin-transferrin-sodium (ITS) supplement. The viability of the cells was assessed with live/dead cells kit (ethidium homodimer-1 and calcein). Cell proliferation was assessed with MTS assay, while multilineage differentiation potential was assessed with human MSC functional identification kit.

Results:
Our results showed that the viability of MSCs was comparable between FBS and ITS supplement groups at 24 h and 48 h intervals. Both groups showed similar proliferation capacity after 48 h and 72 h of incubation with no significant statistical difference. The cells from both groups were able to differentiate into osteoblasts, adipocytes, and chondrocytes.

Conclusion:
Insulin-transferrin-selenium supplement could be used as an alternative to FBS in laboratory experiments and clinical applications (such as cell therapy) to avoid the drawbacks of FBS and enhance the outcome of these applications.
Impact of Various Hematological and Biochemical Parameters in Severe and Nonsevere COVID-19 Patients
Authors: Banerjee Aurobinda , Banerjee Jayashree , Mazumdar Mohua , Phukan Jyoti Prakash 
Published: January 2023

Background:
COVID-19 infection has been declared a pandemic in 2020 and since then it has been affecting the vast population of the world till today. Alteration of various hematological and biochemical parameters has been documented in various literatures.

Aim:
The aim of our study is to find out the prognostic role of hematological and biochemical parameters in severe and nonsevere COVID-19 patients.

Materials and Methods:
It was a retrospective record-based study conducted in a district-level COVID hospital of West Bengal, India. The COVID-confirmed patients admitted to the hospital during the second wave of COVID were included and data from records, including laboratory investigations were collected from the hospital registry. The severity of COVID was categorized based on the national guidelines. Independent samples t-test was applied to find out any relation of hematological and biochemical parameters abnormality in relation to disease severity. P < 0.05 was considered statistically significant.

Results:
A total of 94 COVID-confirmed cases were included in our study. Males constitute the majority of cases (51 males vs. 43 females). Seventeen cases were categorized as severe COVID (18.09%), whereas others were nonsevere COVID. Among hematological parameters, total leukocyte count (TLC), neutrophil and lymphocyte percentage, and neutrophil-to-lymphocyte ratio (NLR) were significantly altered among the severe compared to the nonsevere group (P < 0.05). While C-reactive protein (CRP), D-dimer, and serum ferritin levels also showed significant alteration among severe COVID patients (P < 0.05).

Conclusion:
Our study showed that severe COVID patients were significantly associated with neutrophilia, raised NLR, CRP, D-dimer, and serum ferritin levels.
Pheromone-based monitoring of invasive alien insects along the border of Finland and Russia – methods and unintentionally caught species
Authors: Melin Markus , Vihervuori Liisa , Koivula Matti , Velmala Sannakajsa 
Published: January 2023

Global trade provides pathways for the spread of invasive species. To tackle the threat, many countries have designated surveys that are typically conducted at the probable ports of entry. For Finland, the most north-eastern region of the European Union (EU), such site is the border with Russia and the imports of coniferous roundwood and wood chips. In this paper, we describe the monitoring systems based on pheromone-trapping for three EU-wide quarantine pests: Dendrolimus sibiricus, Polygraphus proximus and Bursaphelenchus xylophilus. We also list the non-target species caught in an exploratory survey using pheromone traps. During the three years of survey, no quarantine pests were detected, but 30 other species of insects were caught. Therefore, the monitoring – despite not detecting the target pests – provided information about the abundance of other species. As insect diversity reflects the status of the surrounding environment, the value of such data should be increased via co-operation among research institutes.
Keywords: wood import, invasive alien species, monitoring, pest, forest damage, insect
Sobre a gênese do pensamento econômico do Brasil: aspectos da economia colonial
Authors: Lunardi do Carmo Marcelo 
Published: January 2023

Resenha do Livro:
PAULA, João Antônio de. Capítulos de história do pensamento econômico do Brasil. Prefácio: Maria Alice Rosa Ribeiro. São Paulo: Hucitec: ABPHE, 2021.
KONFORMITAS DENGAN BRAND SWITCHING PADA MEREK HANDPHONE REMAJA
Authors: Hurriyati Dwi , Mardiansyah Arif 
Published: January 2023

Abstrak
Penelitian ini bertujuan untuk mengetahui hubungan antara konformitas dengan brand switching pada remaja di sukabangun I. Hipotesis yang diajukan dalam penelitian ini adalah ada hubungan antara konformitas dengan brand switching pada remaja di sukabangun I.
Jumlah populasi dalam penelitian ini sebanyak 150 remaja dan yang dijadikan sampel sebanyak 105 remaja yang didapat melalui teknik purposive random sampling. Alat ukur yang digunakan dalam penelitian ini adalah skala konformitas dan skala brand switching. Teknik analisis menggunakan teknik analisis regresi sederhana dengan bantuan SPSS versi 20.
Hasil analisis data penelitian dengan komputer menggunakan program SPSS versi 20 for windows, menunjukkan koefisien korelasi (r) sebesar 0,261 koefisien determinasi (R Square) sebesar 0,068 serta nilai p = 0,007 p < 0,05 pada uji nova dua jalur. Hal ini menunjukan bahwa hipotesis diterima. Hasil analisis tersebut menunjukkan bahwa ada hubungan yang signifikan antara konformitas dengan brand switching pada remaja di sukabangun I . sumbangan variabel bebas terhadap variabel terikat sebesar 6,8%.

Kata Kunci: konformitas, Brand Switching, Remaja
Abstract
This study aims to determine the relationship between conformity and brand switching in adolescents in Sukabangun I. The hypothesis proposed in this study is that there is a relationship between conformity and brand switching in adolescents in Sukabangun I.
The population in this study were 150 adolescents and 105 adolescents were sampled through purposive random sampling technique. The measuring instruments used in this research are conformity scale and brand switching scale. The analysis technique uses simple regression analysis techniques with the help of SPSS version 20.
The results of research data analysis using the SPSS version 20 for windows program showed a correlation coefficient (r) of 0.261 coefficient of determination (R Square) of 0.068 and p = 0.007 p <0.05 in the two-way Nova test. This shows that the hypothesis is accepted. The results of the analysis indicate that there is a significant relationship between conformity and brand switching among adolescents in Sukabangun I. the contribution of the independent variable to the dependent variable is 6.8%

Keywords : Conformity, Brand Switching, Adolescents
KOMUNIKASI DAKWAH HIMPUNAN PEMUDA ALKHAIRAAT (HPA) DALAM PEMBINAAN KEHIDUPAN BERAGAMA REMAJA DI KOTA PALU
Authors: Aldiawan  
Published: January 2023

Penelitian ini pembahasannya bersifat kualitatif berupaya mengumpulkan data dengan melakukan pendekatan komunikasi kelompok (group communication) untuk menjelaskan secara mendalam terkait dengan komunikasi dakwah yang digunakan HPA dalam pembinaan kehidupan beragama remaja di kota palu, sehingga dapat terwujud komunikasi dakwah yang tepat terhadap remaja dan juga menjelaskan faktor-faktor pendukung dan penghambat dari pelaksanaan komunikasi dakwah HPA dalam pembinaan kehidupan beragama remaja di kota palu. Setelah melakukan penelitian maka hasil temuan dirumuskan sebagai berikut: komunikasi dakwah HPA dalam pembinaan kehidupan beragama remaja di kota palu dengan menggunakan metode al-Mujadalah (diskusi-diskusi keagamaan) meliputi: kajian keagamaan berbasis toleransi, seminar sosial keagamaan dan dialog-dialog keagamaan melalui kegiatan pemutaran film dan video yang bernuansa Islam serta mengadakan kegiatan pengkaderan terhadap remaja di kota palu.

Kata Kunci : Komunikasi Dakwah, HPA, Pembinaan Remaja
POLA KOMUNIKASI POLITIK REMAJA DI KECAMATAN RAPPOCINI MELALUI PENGGUNAAN MEDIA SOSIAL
Authors: Muhammad Rustam  , Pala Rukman 
Published: January 2023

Untuk melihat pola perubahan komunikasi politik remaja di Kecamatan Rappocini melalui penggunaan media sosial, peneliti menggunakan mixed methodology yaitu memadukan antara metode survey (kuantitaf) dengan metode kualitatif focus group discussion (FGD). Dalam penelitian ini, peneliti melakukan survey pada 50 responden yang terdiri dalam kategori remaja di Kecamatan Rappocini. Selanjutnya, untuk mendapatkan temuan data yang mendalam, peneliti mengelaborasi hasil temuan survey melalui focus group discussion (FGD) bersama 5 orang responden yang berpotensi menjadi narasumber yang mantab. Pemilihan responden FGD menggunakan purposive sampling. Penelitian ini diharapkan mampu memberikan gambaran pola perubahan komunikasi politik remaja di Kecamatan Rappocini melalui penggunaan media sosial. Keterlibatan remaja pada partisipasi politik dalam konteks komunikasi politik dengan adanya media sosial yang hidup dan berkembang di tengah-tengah mereka. Selain itu, penelitian ini juga diharapakan dapat memberikan kontribusi bagi perkembangan ilmu komunikasi khususnya dalam konteks komunikasi politik dalam era media baru, terlebih untuk memetakan partisipan politik yang berasal dari kalangan remaja sebagai pengguna aktif media sosial.