bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of multiparametric MRI based prediction model in identification of pseudoprogression in glioblastomas
Authors: de Godoy Laiz Laura , Mohan Suyash , Wang Sumei , Nasrallah MacLean P. , Sakai Yu , O’Rourke Donald M. , Bagley Stephen , Desai Arati , Loevner Laurie A. , Poptani Harish , Chawla Sanjeev 
Published: April 2023

Abstract
Background
Accurate differentiation of pseudoprogression (PsP) from tumor progression (TP) in glioblastomas (GBMs) is essential for appropriate clinical management and prognostication of these patients. In the present study, we sought to validate the findings of our previously developed multiparametric MRI model in a new cohort of GBM patients treated with standard therapy in identifying PsP cases.

Methods
Fifty-six GBM patients demonstrating enhancing lesions within 6 months after completion of concurrent chemo-radiotherapy (CCRT) underwent anatomical imaging, diffusion and perfusion MRI on a 3 T magnet. Subsequently, patients were classified as TP + mixed tumor (n = 37) and PsP (n = 19). When tumor specimens were available from repeat surgery, histopathologic findings were used to identify TP + mixed tumor (> 25% malignant features; n = 34) or PsP (< 25% malignant features; n = 16). In case of non-availability of tumor specimens, ≥ 2 consecutive conventional MRIs using mRANO criteria were used to determine TP + mixed tumor (n = 3) or PsP (n = 3). The multiparametric MRI-based prediction model consisted of predictive probabilities (PP) of tumor progression computed from diffusion and perfusion MRI derived parameters from contrast enhancing regions. In the next step, PP values were used to characterize each lesion as PsP or TP+ mixed tumor. The lesions were considered as PsP if the PP value was < 50% and TP+ mixed tumor if the PP value was ≥ 50%. Pearson test was used to determine the concordance correlation coefficient between PP values and histopathology/mRANO criteria. The area under ROC curve (AUC) was used as a quantitative measure for assessing the discriminatory accuracy of the prediction model in identifying PsP and TP+ mixed tumor.

Results
Multiparametric MRI model correctly predicted PsP in 95% (18/19) and TP+ mixed tumor in 57% of cases (21/37) with an overall concordance rate of 70% (39/56) with final diagnosis as determined by histopathology/mRANO criteria. There was a significant concordant correlation coefficient between PP values and histopathology/mRANO criteria (r = 0.56; p < 0.001). The ROC analyses revealed an accuracy of 75.7% in distinguishing PsP from TP+ mixed tumor. Leave-one-out cross-validation test revealed that 73.2% of cases were correctly classified as PsP and TP + mixed tumor.

Conclusions
Our multiparametric MRI based prediction model may be helpful in identifying PsP in GBM patients.
Clinical characteristics and risk factors of coronary artery lesions in chinese pediatric Takayasu arteritis patients: a retrospective study
Authors: Xu Yingjie , Luo Lingfeng , Su Gaixiu , Zhu Jia , Kang Min , Zhang Dan , Lai Jianming , Li Xiaohui 
Published: April 2023

Abstract
Backgroud
To summarize the clinical characteristics and identify the risk factors for pediatric Takayasu arteritis (TAK) with coronary artery lesions (CALs).

Methods
Clinical data of pediatric TAK patients in our center were retrospectively assessed. Independent risk factors for CALs were identified using multivariate logistic regression analysis. Survival analysis was used to compare differences in survival rates between the groups.

Results
Among the 66 pediatric TAK cases, the incidence of accompanying CALs was 39.4%. In the CAL group, 19 (73.1%) cases started within 36 months. None of the patients had symptoms of angina or ischemia on electrocardiogram (ECG), the CALs were detected using coronary ultrasound. The CALs most commonly were the left main and right coronary arteries. The lesions were mostly small or middle coronary artery aneurysms; some children may have giant coronary aneurysmal dilations, thrombosis and heart failure. The age of onset and symptom onset to diagnosis in TAK patients with CAL were lower than those in TAK patients without CAL(P < 0.005). TAK patients with CAL had significantly higher CRP,WBC, PLT,TNF-α and IL-2R levels (P < 0.05), lower HGB (P = 0.01), lower rate of renal artery stenosis (RAS) (P = 0.009). In multivariate logistic regression, the risk factors for pediatric TAK combined with CAL included the age of TAK onset (OR = 0.9835, 95% CI: 0.9710–0.9946, P = 0.006) and RAS (OR = 0.1901, 95% CI: 0.0386–0.7503, P = 0.03). In addition, there was no significant difference in survival rates between the two groups after regular treatment.

Conclusion
This study showed that the occurrence of CAL in pediatric TAK patients has a relatively more rapid clinical course, and a stronger inflammatory state at the time of diagnosis. The earlier the age of TAK onset and without RAS are more likely to cause CAL.
PLPP/CIN-mediated NF2 S10 dephosphorylation distinctly regulates kainate-induced seizure susceptibility and neuronal death through PAK1-NF-κB-COX-2-PTGES2 signaling pathway
Authors: Kim Ji-Eun , Lee Duk-Shin , Kim Tae-Hyun , Park Hana , Kim Min-Ju , Kang Tae-Cheon 
Published: April 2023

Abstract
Background
Pyridoxal-5′-phosphate phosphatase/chronophin (PLPP/CIN) selectively dephosphorylates serine (S) 10 site on neurofibromin 2 (NF2, also known as merlin (moesin-ezrin-radixin-like protein) or schwannomin). p21-activated kinase 1 (PAK1) is a serine/threonine protein kinase, which is involved in synaptic activity and plasticity in neurons. NF2 and PAK1 reciprocally regulate each other in a positive feedback manner. Thus, the aim of the present study is to investigate the effects of PLPP/CIN-mediated NF2 S10 dephosphorylation on PAK1-related signaling pathways under physiological and neuroinflammatory conditions, which are largely unknown.

Methods
After kainate (KA) injection in wild-type, PLPP/CIN−/− and PLPP/CINTg mice, seizure susceptibility, PAK1 S204 autophosphorylation, nuclear factor-κB (NF-κB) p65 S276 phosphorylation, cyclooxygenase-2 (COX-2) upregulation, prostaglandin E synthase 2 (PTGES2) induction and neuronal damage were measured. The effects of 1,1'-dithiodi-2-naphthtol (IPA-3, a selective inhibitor of PAK1) pretreatment on these responses to KA were also validated.

Results
PLPP/CIN overexpression increased PAK1 S204 autophosphorylation concomitant with the enhanced NF2 S10 dephosphorylation in hippocampal neurons under physiological condition. Following KA treatment, PLPP/CIN overexpression delayed the seizure on-set and accelerated PAK1 S204 phosphorylation, NF-κB p65 S276 phosphorylation, COX-2 upregulation and PTGES2 induction, which were ameliorated by PLPP/CIN deletion or IPA-3. Furthermore, IPA-3 pretreatment shortened the latency of seizure on-set without affecting seizure severity (intensity) and ameliorated CA3 neuronal death induced by KA.

Conclusions
These findings indicate that PLPP/CIN may regulate seizure susceptibility (the latency of seizure on-set) and CA3 neuronal death in response to KA through NF2-PAK1-NF-κB-COX-2-PTGES2 signaling pathway.
The challenges in protecting public health interests in multisectoral governance in the context of small island developing states: the case of tobacco control in Fiji and Vanuatu
Authors: Patay Dori , Schram Ashley , Friel Sharon 
Published: April 2023

Abstract
Background
The commercial determinants of health (CDoH) drive the rise of NCDs globally, and their regulation requires multisectoral governance. Despite existing recommendations to strengthen institutional structures, protecting public health interests can be challenging amidst industry interference and conflicting policy priorities, particularly in low and middle-income countries (LMICs) where the need for rapid economic development is pronounced. Small island developing states (SIDS) face even more challenges in regulating CDoH because their unique socioeconomic, political, and geographic vulnerabilities may weaken institutional conditions that could aid health sector actors in protecting health interests. This study aims to explore the institutional conditions that shape health sector actors’ capability to protect public health interests in tobacco governance in Fiji and Vanuatu.

Methods
We employed a qualitative, exploratory case study design. We applied the administrative process theory to inform data collection and analysis. Seventy interviews were completed in Fiji and Vanuatu from 2018 to 2019.

Results
The findings show that the protection of health interests in tobacco governance were not supported by the institutional conditions in Fiji and Vanuatu. While the policy processes formally ensured a level playing field between actors, policies were often developed through informal mechanisms, and the safeguards to protect public interests from vested private interests were not implemented adequately. SIDS vulnerabilities and weak regulation of political parties contributed to the politicisation of government in both states, resulting in high-level government officials’ questionable commitment to protect public health interests. The system of checks and balances usually embedded into democratic governments appeared to be muted, and policymakers had limited bureaucratic autonomy to elevate health interests in multisectoral policymaking amidst high-level government officials’ frequent rotation. Finally, capacity constraints aggravated by SIDS vulnerabilities negatively impacted health sector actors' capability to analyse policy alternatives.

Conclusions
Health sector actors in Fiji and Vanuatu were not supported by institutional conditions that could help them protect public health interests in multisectoral governance to regulate CDoH originating from the tobacco industry. Institutional conditions in these states were shaped by SIDS vulnerabilities but could be improved by targeted capacity building, governance and political system strengthening.
Streptococcus canis prevalence on the normal and abnormal ocular surface of dogs referred for ophthalmic disease in Canada
Authors: Cloet Allyssa , da Silva Arthur Nery , Facioli Fernanda Luiza , Levitt Shayna , Sandmeyer Lynne Sheila , de Oliveira Costa Matheus , Leis Marina Laetitia 
Published: April 2023

AbstractStreptococcus canisis a beta-haemolytic, Gram-positive cocci commonly identified on the canine ocular surface under both healthy and diseased conditions. The objective of the study was to determine the prevalence ofS. canison the normal and abnormal ocular surface of a canine ophthalmology referral population in Canada, and to investigate potential clinical aspects that may be associated with its presence. Included were 59 dogs (118 eyes) with unilateral or bilateral ocular disease diagnosed at the time of conjunctival sampling. A real-time PCR specific forS. caniswas standardized for use with conjunctival swabs. Total DNA was extracted from 118 samples and used as template for the diagnostic assay. Samples were considered positive if amplification was detected and dissociation temperature matched a positive control. Signalment and other clinical data were also collected at the time of sampling. Of the 118 eyes sampled, 8 tested positive forS. canis(6.8%). No association between the detection ofS. canisand breed, cephalic conformation, sex, age, use of ophthalmic antibiotics or other topical medications, ophthalmic diagnosis, use of systemic antibiotics or other systemic medications, or systemic diagnosis was identified. In conclusion,S. canismay be present on the ocular surface of dogs at a higher rate than previously reported. It is suggested that this may be linked to the use of PCR for pathogen detection instead of culture.
Identification of key biomarkers associated with immune cells infiltration for myocardial injury in dermatomyositis by integrated bioinformatics analysis
Authors: Zhang Yue , Shan Linwei , Li Dongyu , Tang Yinghong , Qian Wei , Dai Jiayi , Du Mengdi , Sun Xiaoxuan , Zhu Yinsu , Wang Qiang , Zhou Lei 
Published: April 2023

Abstract
Background
Dermatomyositis (DM) is an acquired autoimmune disease that can cause damage to various organs, including the heart muscle. However, the mechanisms underlying myocardial injury in DM are not yet fully understood.

Methods
In this study, we utilized publicly available datasets from the Gene Expression Omnibus (GEO) database to identify hub-genes that are enriched in the immune system process in DM and myocarditis. Weighted gene co-expression network analysis (WGCNA), differentially expressed genes (DEGs) analysis, protein–protein interaction (PPI), and gene ontology (GO) analysis were employed to identify these hub-genes. We then used the CIBERSORT method to analyze immune cell infiltration in skeletal muscle specimens of DM and myocardium specimens of myocarditis respectively. Correlation analysis was performed to investigate the relationship between key genes and infiltrating immune cells. Finally, we predicted regulatory miRNAs of hub-genes through miRNet and validated their expression in online datasets and clinical samples.

Results
Using integrated bioinformatics analysis, we identified 10 and 5 hub-genes that were enriched in the immune system process in the database of DM and myocarditis respectively. The subsequent intersections between hub-genes were IFIT3, OAS3, ISG15, and RSAD2. We found M2 macrophages increased in DM and myocarditis compared to the healthy control, associating with the expression of IFIT3, OAS3, ISG15, and RSAD2 in DM and myocarditis positively. Gene function enrichment analysis (GSEA) showed that IFIT3, OAS3, ISG15, and RSAD2 were mainly enriched in type I interferon (IFN) signaling pathway, cellular response to type I interferon, and response to type I interferon. Finally, we verified that the expression of miR-146a-5p was significantly higher in the DM with myocardial injury than those without myocardial injury (p = 0.0009).

Conclusion
Our findings suggest that IFIT3, OAS3, ISG15, and RSAD2 may play crucial roles in the underlying mechanism of myocardial injury in DM. Serum miR-146a-5p could be a potential biomarker for myocardial injury in DM.
Identification of mutations that causes glucose-6-phosphate transporter defect in tunisian patients with glycogenosis type 1b
Authors: Chkioua Latifa , Amri Yessine , Sahli Chayma , Rhouma Ferdawes Ben , Chehida Amel Ben , Tebib Neji , Messaoud Taieb , Abdennebi Hassen Ben , Laradi Sandrine 
Published: April 2023

Abstract
Background
Glycogen storage disease type 1b (GSD1b) is an autosomal recessive lysosomal storage disease caused by defective glucose-6-phosphate transporter encoded by SLC37A4 leading to the accumulation of glycogen in various tissues. The high rate of consanguineous marriages in Tunisian population provides an ideal environment to facilitate the identification of homozygous pathogenic mutations. We aimed to determine the clinical and genetic profiles of patients with GSD1b to evaluate SLC37A4 mutations spectrum in Tunisian patients.

Methods
All exons and flanking intron regions of SLC37A4 gene were screened by direct sequencing to identify mutations and polymorphisms in three unrelated families with GSD1b. Bioinformatics tools were then used to predict the impacts of identified mutations on the structure and function of protein in order to propose a function-structure relationship of the G6PT1 protein.

Results
Three patients (MT, MB and SI) in Families I, II and III who had the severe phenotype were homoallelic for the two identified mutations: p.R300H (famillies I, II) and p.W393X (Family III), respectively. One of the alterations was a missense mutation p.R300H of exon 6 in SLC37A4 gene. The analysis of the protein structure flexibility upon p.R300H mutation using DynaMut tool and CABS-flex 2.0 server showed that the reported mutation increase the molecule flexibility of in the cytosol region and would probably lead to significant conformational changes.

Conclusion
This is the first Tunisian report of SLC37A4 mutations identified in Tunisia causing the glycogenosis type Ib disease. Bioinformatics analysis allowed us to establish an approximate structure-function relationship for the G6PT1 protein, thereby providing better genotype/phenotype correlation knowledge.
The role of wnt signaling in diabetes-induced osteoporosis
Authors: Bao Kairan , Jiao Yinghua , Xing Lei , Zhang Fang , Tian Faming 
Published: April 2023

AbstractOsteoporosis, a chronic complication of diabetes mellitus, is characterized by a reduction in bone mass, destruction of bone microarchitecture, decreased bone strength, and increased bone fragility. Because of its insidious onset, osteoporosis renders patients highly susceptible to pathological fractures, leading to increased disability and mortality rates. However, the specific pathogenesis of osteoporosis induced by chronic hyperglycemia has not yet been fully elucidated. But it is currently known that the disruption of Wnt signaling triggered by chronic hyperglycemia is involved in the pathogenesis of diabetic osteoporosis. There are two main types of Wnt signaling pathways, the canonical Wnt signaling pathway (β-catenin-dependent) and the non-canonical Wnt signaling pathway (non-β-catenin-dependent), both of which play an important role in regulating the balance between bone formation and bone resorption. Therefore, this review systematically describes the effects of abnormal Wnt pathway signaling on bone homeostasis under hyperglycemia, hoping to reveal the relationship between Wnt signaling and diabetic osteoporosis to further improve understanding of this disease.
Newton’s second law as limit of variational problems
Authors: Percivale Danilo , Mainini Edoardo 
Published: April 2023

AbstractWe show that the solution of Cauchy problem for the classical ODE $m \mathbf {y}''=\mathbf {f}$
m

y
″

=
f
can be obtained as the limit of minimizers of exponentially weighted convex variational integrals. This complements the known results about weighted inertia-energy approach to Lagrangian mechanics and hyperbolic equations.
Load balancing scheduling mechanism for OpenStack and Docker integration
Authors: Qian Jiarui , Wang Yong , Wang Xiaoxue , Zhang Peng , Wang Xiaofeng 
Published: April 2023

AbstractWith the development of cloud-edge collaborative computing, cloud computing has become crucial in data analysis and data processing. OpenStack and Docker are important components of cloud computing, and the integration of the two has always attracted widespread attention in industry. The scheduling mechanism adopted by the existing fusion solution uses a uniform resource weight for all containers, and the computing nodes resources on the cloud platform is unbalanced under differentiated resource requirements of the containers. Therefore, considering different network communication qualities, a load-balancing Docker scheduling mechanism based on OpenStack is proposed to meet the needs of various resources (CPU, memory, disk, and bandwidth) of containers. This mechanism uses the precise limitation strategy for container resources and a centralized strategy for container resources as the scheduling basis, and it generates exclusive weights for containers through a filtering stage, a weighing stage based on weight adaptation, and a non-uniform memory access (NUMA) lean stage. The experimental results show that, compared with Nova-docker and Yun, the proposed mechanism reduces the resource load imbalance within a node by 57.35% and 59.00% on average, and the average imbalance between nodes is reduced by 53.53% and 50.90%. This mechanism can also achieve better load balancing without regard to bandwidth.
Moved by Another Life: Altered Sentience and Historical Poiesis in the Peyote Craze
Authors: Boulette Sylvie 
Published: April 2023

Abstract
This article follows the transsensory pathways opened by peyote, a mood-altering entheogen, as it diffused among Native peoples living under and along the edges of colonial occupation around the turn of the twentieth century. It traces that movement through the pulsions of temporal and sensorial animacy created in the episodic narration of The Autobiography of a Winnebago Indian (1920), the life story of a Ho-Chunk man named Sam Blowsnake. Apprehended by settler governance as a “craze,” anti-assimilationist currents of the Peyote movement met with intensifying attempts to constrain the biochemical flux of “intoxication” through Native bodies. Modern campaigns against “peyote worship” mounted by government agents, progressive reformers, and sensationalist press in the early twentieth century exploited scripts of revulsion and prurient fascination to target the border-crossing “mind poison” for prohibition. Just beneath the scenes of psychotoxicity and sexual disorder projected by the antinarcotic imaginary of the craze, this article posits, there ran an ongoing struggle over the restrictive capacitation of property and personhood within the settler-capitalist regime of allotment. Against the norms enforced by that policy, in peyote meetings the alteration of sentience could unbind the day-to-day reproduction of property-bearing personhood. Lines of collective transport opening from the passage of ecstasy thus composed a historical moment in refusal of allotment drives for schizochronic individuation.
Diversity of Penaeidae at the Mengkudu Bay Waters, North Sumatra, Indonesia
Authors: AZWAR EDI , SULARNO SULARNO , WARUWU FRANSISKUS PERIODE , TARIGAN MHD. RAFI’I MA’ARIF , ULFA SYARIFAH WIDYA , DJAINGSASTRO AULIA JUANDA 
Published: April 2023

Abstract. Azwar E, Sularno, Waruwu FP, Tarigan MRM, Ulfa SW, Djaingsastro AJ. 2023. Diversity of Penaeidae at the Teluk Mengkudu Waters, North Sumatra, Indonesia. Biodiversitas 24: 1376-1384. Microscopic animals play an important role in the growth of marine biota. Crustaceans are a marine biota group often found in waters and have high economic value. Crustaceans also have a significant role in coral reef ecosystems, contributing around 20% of all invertebrate species. Crustaceans are an important group of microbenthic fauna in coastal ecosystems, ranging from mangroves, coral reefs, and seagrass ecosystems. This present study was conducted from April to June 2022 to identify Penaeidae diversity in Teluk Mengkudu waters, Serdang Bedagai District, North Sumatra Province, Indonesia. Furthermore, it used a purposive sampling method to harvest crustacean species near the waters. An exploratory method was applied with quantitative data collection to select Penaeidae crustaceans from the catches retrieved by fishermen. The tools used in this study were boat, net, fiber, knife, compass, flags, camera, notebook, ice cubes, a thermometer, pH meter, and DO meter. The material used in this study was shrimp caught by fishers. Data collection techniques used interviews and documentation with fishing communities about catching crustaceans at the study site. Data analysis uses the formula diversity, evenness, knowledge richness, and dominance index. Based on the results, Penaeus indicus H.Milne Edwards, 1837 and Metapenaeus ensis (De Haan, 1844) were the two species obtained from the Penaeidae family. The evenness index for P. indicus and M. ensis was respectively highest in June (0.237 and 0.519) but lowest in May (0.203 and 0.510) with a total value of 2.212. Also, the prominent richness index for both species was respectively highest in June (5.762 and 29.371) but lowest in May (4.568 and 27.053). The highest dominance index (D) was measured in May, where the highest D value of P. indicus was 0.003, and the lowest was 0.002. The highest D of M. ensis was discovered in June, namely 0.086, while the lowest, at 0.073, was recorded in May, and in general, both species had a total D of 0.245. Parameters of salinity, pH, temperature, substrate, and oxygen strongly influence crustacean diversity. The parameters measured in the study area included temperature (28?), salinity (20-35 ppt), DO (6.8 mg/L), and pH (6.7).