bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-224-5p inhibits proliferation and invasion of non-small cell lung cancer by targeting IL6ST inactivation of JAK2/STAT3 signal pathway
Authors: Li Xiaoping , Tian Jiao , Xiao Ting , He Yiming , Zhang Zihui , Zhu Yuxin , Ren Haixia , Zhang Liang , Li Lei , Li Wei , Zhang Weidong , Zhou Honggang 
Published: February 2023

Abstract
Non-small cell lung cancer (NSCLC) accounts for more than 85% of lung cancer, with high morbidity and mortality. Studies have shown that microRNA can specifically inhibit the progression of NSCLC. MiR-224-5p can regulate tumor progression in many cancers, but its function and mechanism in NSCLC aren’t clear. In this study, we found that the expression of miR-224-5p was reduced in NSCLC tissue and cells than normal lung tissue and cells. At the same time, miR-224-5p negatively regulates the proliferation and migration of NSCLC cells. Inhibition of miR-224-5p expression in A549 cells could promote cell proliferation, invasion, migration and VM formation in vitro and tumor growth and lung metastasis in vivo, while over expression of miR-224-5p in H226 cells reversed the effect. Besides, we predicted target gene and found that IL6ST is a potential target gene of miR-224-5p. The expression of miR-224-5p is negatively correlated with IL6ST and activation of JAK2/STAT3 pathway. Over expression of IL6ST reversed the effects of miR-224-5p on migration, invasion and activation of JAK2/STAT3 pathway in H226 cells. In conclusion, this study revealed that miR-224-5p can inhibit the proliferation and invasion of NSCLC by targeting inhibition of IL6ST gene transcriptional and inactivation of JAK2/STAT3 signal pathway.
A Pattern of Stable Isotopes and a Toroidal Helical Wave Orbital Model
Authors: Lin Jingfeng , Lin Guangyi 
Published: February 2023

Abstract
We investigate a pattern of isotopes with the highest isotopic abundance, the number of nucleons of some stable isotopes tends to A=S(S+1)-2, this means that S is probably the number of shells. Therefore, we propose a nucleon arrangement model that can include all magic numbers. Furthermore, based on some simple patterns in atomic nuclei, such as shell structures, magic numbers, magnetic moment and collective modes, we developed a toroidal helical wave orbital model to explore the microstructure of isotopes that matches the proposed arrangement pattern.
Surprising sex differences indicate davunetide-mediated brain protection and clinical efficacy in women suffering from progressive supranuclear palsy
Authors: Gozes Illana , Shapira Guy , Lobyntseva Alexandra , Shomron Noam 
Published: February 2023

Abstract
Progressive supranuclear palsy (PSP) is a pure tauopathy, implicating davunetide, enhancing Tau-microtubule interaction, as an ideal drug candidate. However, mixing men and women concluded no efficacy. In contrast, here, analyzing sex-dependency in a comprehensive 52-weeks PSP clinical trial (313 patients), showed clear baseline differences in brain ventricular volumes. Significant age/decreased ventricular volume correlations in davunetide-treated females, and highly significant baseline ventricular volume-dependent/volume increase correlations in placebo-treated females were observed, while males showed no effects. Using sophisticated statistics, female-specific davunetide-mediated protection of ventricular volume was directly correlated with primary trial-endpoints, Schwab and England Activities of Daily Living (SEADL), and PSP Rating Scale (PSPRS). Davunetide-treated females showed slower deterioration in SEADL, and in the bulbar domain of PSPRS including speaking and swallowing difficulties caused by brain damage. Together with slower disease progression seen in men, the results reveal gender-based drug efficacy, demonstrating the neuroprotective, disease-modifying impact of davunetide treatment in female PSP patients.
Galectin-9 has non-apoptotic cytotoxic activity towards Acute Myeloid Leukemia independent of cytarabine resistance
Authors: Wiersma Valerie , Choukrani Ghizlane , Visser Nienke , Avtenyuk Natasha Ustyanovska , Olthuis Mirjam , Marsman Glenn , Ammatuna Emanuele , Lourens Harm Jan , Niki Toshiro , Huls Gerwin , Bremer Edwin 
Published: February 2023

Abstract
Acute myeloid leukemia (AML) is a malignancy still associated with poor survival rates, among others due to frequent occurrence of therapy-resistant relapse after standard-of-care treatment with cytarabine (AraC). AraC triggers apoptotic cell death, a type of cell death to which AML cells often become resistant. Therefore, therapeutic options that trigger an alternate type of cell death are of particular interest. We previously identified that the glycan-binding protein Galectin-9 (Gal-9) has tumor-selective and non-apoptotic cytotoxicity towards various types of cancer, which depended on autophagy inhibition. Thus, Gal-9 could be of therapeutic interest for (AraC-resistant) AML. In the current study, treatment with Gal-9 was cytotoxic for AML cells, including for CD34+ patient-derived AML stem cells, but not for healthy cord blood-derived CD34+ stem cells. This Gal-9-mediated cytotoxicity did not rely on apoptosis but negatively associated with autophagic flux. Importantly, both AraC-sensitive and -resistant AML cell lines as well as AML patient samples were sensitive to single agent treatment with Gal-9. Additionally, Gal-9 potentiated the cytotoxic effect of DNA demethylase inhibitor Azacytidine (Aza), a drug that is clinically used for patients that are not eligible for intensive AraC treatment. Thus, Gal-9 is a potential therapeutic agent for the treatment of AML, including AraC resistant AML, by inducing caspase-independent cell death.
Finite Element Analysis of Metal and Nonmetal Strain Wave Gear
Authors: Yu Zhiyuan 
Published: February 2023

Abstract
This paper studies the feasibility of using polymer for strain wave gear based on finite element analysis (FEA). The FEA model includes strain wave gear’s three major components: flexspline, circular spline, and wave generator. It uses high quality quadrilateral elements for the three-dimensional tooth surfaces, which have conjugated tooth profile and its modifications. The simulation has been verified by a prototype strain wave gear’s contact pattern testing. A theoretical model to analyze the strain wave gear’s stress has been established to guide the selection of high-performance polymers for the strain wave gear. A sample design has been studied to comparing the polymer and metal strain wave gear. The simulation results show that polymer can reduce the strain wave gear’s circumferential stress by up to 98%, and torque induced stress by up to 70% at rated torque. The factor of safety against static load reduces by 21%, however the fatigue factory of safety increases by 16% according to Soderberg criteria. The polymer strain wave gear’s torsional stiffness is lower than metal, and the transmission error at different torque is characterized by the FEA simulation. Due to strain wave gear’s operating theory, polymer has unique advantages when used for strain wave gears than used for solid gears. Polymer’s flexibility will directly reduce the stress induced by flexspline’s designed deformation, and reduce the stress induced by torque load because of the contact ratio’s increase. Together with properties such as light weight, high specific strength, self-lubrication, and low material and manufacturing cost, high performance polymer is an ideal material candidate for strain wave gears.
Prevalence of COVID-19 and Long COVID in Collegiate Student Athletes from Spring 2020 to Fall 2021: A Retrospective Survey
Authors: Massey Daisy , Saydah Sharon , Adamson Blythe , Lincoln Andrew , Aukerman Douglas F. , Berke Ethan M. , Sikka Robby , Krumholz Harlan M. 
Published: February 2023

Abstract
Background. Symptomatic COVID-19 and post-COVID conditions, also referred to as post-acute sequelae of SARS-CoV-2 (PASC) or Long COVID, have been widely reported in young, healthy people, but their prevalence has not yet been determined in student athletes. We sought to estimate the prevalence of reported COVID-19, symptomatic COVID-19, and Long COVID in college athletes in the United States attending 18 schools from spring 2020 to fall 2021.
Methods. We developed an online survey to measure the prevalence of student athletes who tested positive for COVID-19, developed Long COVID, and did not return to their sport during the relevant time period. We surveyed a convenience sample of 18 collegiate school administrators, representing about 7,000 student athletes.
Results. According to the survey responses, there were 9.8% of student athletes who tested positive for COVID-19 in spring 2020 and 25.4% who tested positive in the academic year of fall 2020 to spring 2021. About 4% of student athletes who tested positive from spring 2020 to spring 2021 developed Long COVID, defined as new, recurring, or ongoing physical or mental health consequences occurring 4 or more weeks after SARS-CoV-2 infection.
Conclusions. This study highlights that Long COVID occurs among young, healthy athletes and is a real consequence of COVID-19. Understanding the prevalence, duration, and lasting consequences of Long COVID requires longer follow-up and further study.
Ultra-low friction and stiffness dependence of interlayer friction in graphite flakes under various rotation angles
Authors: Peng Yitian , Yu Kang , Xu Peipei , Huang Yao , Lang Haojie , Ding Shuyang 
Published: February 2023

Abstract
The 60-degree rotational symmetry of graphene layers leads to remarkable physical and mechanical phenomena. One corresponds to ultralow sliding friction and friction coefficient between incommensurate graphene layers. A pick-up method was proposed to pick graphite flake to microsphere probe and achieved graphite and graphite tribo-pair. Ultralow friction was attained for various rotation angles, and the friction coefficient was kept at the range of 10− 3. Density functional theory (DFT) calculation was employed to reveal the angular dependence of friction, and the calculations are partially consistent with the experimental results. The difference of angle-dependence ultralow friction between experimental results and DFT calculations was observed, which was greatly influenced by the stiffness of the system. Friction hysteresis, abnormal shape of pull-off curves and long jumps in stick-slip were further demonstrated the stiffness of the system had changed during the friction measurements. The contact geometry in our experiment was a finite, symmetric, nanometer-sized (less than 1nm), flexible graphite flake and a rigid graphite surface. The tribological system offers an experimental platform to study interlayer friction and contact geometry.
Familial Epilepsy Associated with Concurrent CHRNB2 Mutation and RBFOX1 Exon Deletion
Authors: Xu Tony , Dorris Luong , Zhong Ning 
Published: February 2023

Abstract
Background
Understanding the genetic basis of epilepsy may lead to an improved understanding of the etiology, more precise medical management, and ultimately improved outcomes. It is imperative for patients with epilepsy to obtain a molecular diagnosis, especially when a strong familial epilepsy is discovered.
Case presentation
The proband was a 19 years old female who experienced focal onset seizures, with presenting symptoms of feeling dizzy, disorientation, and passing out. Her EEG studies revealed interictally focal slow and sharp waves in either the left or right hemisphere. EEG monitoring data showed that the seizures arose from the left fronto-temporal regions. Her brain MRI was normal. The proband sister also suffered from focal onset seizures. Her EEG showed focal epileptiform discharge in the right temporal regions, and a subsequent brain MRI was unrevealing. Two genetic tests were conducted for the proband: 1) array CGH revealed 16p13.3 deletion but no 22q deletion; and 2) NGS Epilepsy Panel revealed a few variants of uncertain significance (VUS) including in CHRNB2 (c.1423A >G, p.Ile475Val) and RBFOX1 (exon1-2 deletion). The proband’s sister also carries both the CHRNB2 variant and RBFOX1 deletion. The proband’s father carries the CHRNB2 variant her brother and her mother carry the deletion of RBFOX1.
Conclusions
In this family, the co-expression of the CHRNB2 variant and RBFOX1 deletion may cause the clinical seizures seen in the proband and her sister. It is also possible that the RBFOX1 deletion is associated with an increased risk of seizure disorder with variable expressivity.
Age at Surgery and Native Liver Survival in Biliary Atresia: A Systematic Review and Meta-Analysis
Authors: Hoshino Eri , Muto Yamato , Sakai Kotomi , Shimohata Nobuyuki , Urayama Kevin , Suzuki Mitsuyoshi 
Published: February 2023

Abstract
Introduction
Biliary atresia (BA) is a childhood rare disease of the liver and bile ducts that requires prompt surgical intervention. Age at surgery is an important prognostic factor; however, controversy exists with regards to the benefit of early Kasai procedure (KP). We aimed to conduct a systematic review and meta-analysis to examine the relationship between the age at KP and native liver survival (NLS) of BA patients.
Methods
We performed the electronic database search using Pubmed, EMBASE, Cochrane, and Ichushi Web and included all relevant studies published from 1968 up to May 3, 2022. Studies which examined the timing of KP at age 30, 45, 60, 75, 90, 120, and/or 150 days were included. The outcome measures of interest were NLS rates at 5, 10, 15, 20, and 30 years post-KP and the hazard ratio or risk ratio for NLS. The quality assessment was used using the ROBINS-I tool.
Results
Among 1,653 potentially eligible studies, 9 articles met the inclusion criteria for the meta-analysis. Meta-analysis for hazard ratios revealed that there was significantly faster time to liver transplantation in the group of patients who had KP at later timing as compared with earlier KP (HR=2.12, 95% CI 1.51-2.97). The risk ratio comparing KP <30 days and KP >31 days on native liver survival was 1.22 (95%CI 1.13-1.31). The sensitivity analysis showed that comparing KP <30 days and KP 31-60 days, the risk ratio was 1.13, 95%CI 1.04-1.22.
Conclusion
Our meta-analysis showed the importance of early diagnosis and surgical interventions ideally before 30 days of life in infants with BA on native liver survival on 5, 10, and 20 years. Therefore, effective newborn screening of BA targeting KP <30 days is needed to ensure prompt diagnosis of affected infants.
CXCL9 and CXCL13 coordinately shape the immune-activated microenvironment of endometrial cancer via tertiary lymphoid structure formation
Authors: Kodama Makoto , Nagase Yoshihiro , Aimono Eriko , Nakamura Kohei , Takamatsu Reika , Abe Keiko , Yoshimura Takuma , Chiyoda Tatsuyuki , Yamagami Wataru , Aoki Daisuke ,   hnishihara1971@keio.jp muto
Published: February 2023

Abstract

Immune checkpoint inhibitor (ICI) therapy has been successfully applied to various cancers; however, not all patients respond to ICI therapy. Tumors with an immune-activated environment are more responsive to ICIs. To identify the cells and molecules essential to the formation of an immune-activated cancer microenvironment, we focused on the tertiary lymphoid structure (TLS) and performed histological and genomic analyses using endometrial cancer material. In the high immunogenic group, numerous TLSs were observed and CXCL9 and CXCL13 expression levels were markedly increased. We established that CXCL9-positive antigen-presenting and CXCL13-positive follicular dendritic cells were distributed in the T and B cell zone of TLS, respectively. A group of molecules upregulated along with CXCL9 and CXCL13 expression were strongly associated with cellular immunity. These results suggest that CXCL9-expressing antigen-presenting cells and CXCL13-expressing follicular dendritic cells coordinately shape the immune-activated microenvironment through TLS formation.
RCS: a fast path planning algorithm for Unmanned Aerial Vehicles
Authors: Divkoti Mohammad Reza Ranjbar , Nouri-Baygi Mostafa 
Published: February 2023

Abstract
Path planning is a major problem in autonomous vehicles. In recent years, with the increase in applications of Unmanned Aerial Vehicles (UAVs), one of the main challenges is path planning, particularly in adversarial environments. In this paper, we consider the problem of planning a collision-free path for a UAV in a polygonal domain from a source point to a target point. Based on the characteristics of UAVs, we assume two basic limitations on the generated paths: an upper bound on the turning angle at each turning point (maximum turning angle) and a lower bound on the distance between two consecutive turns (minimum route leg length).
We describe an algorithm that runs in O(n2 log n) time and finds a feasible path in accordance with the above limitations, where $n$ is the number of obstacle vertices. As shown by experiments, the output of the algorithm is much close to the shortest path with this requirement (always below about 10% of the shortest path), in a much smaller time (up to 10000 quicker, in our experiments). We further demonstrate how to decompose the algorithm into two phases, a preprocessing and a query phase. In this way, given a fixed start point and a set of obstacles, we can preprocess a data structure of size O(n2) in O(n2 log n) time, such that for any query target point we can find a path with the given requirements in O(n log n) time. Finally, we modify the algorithm to find a feasible (almost shortest) path that reaches the target point within a given range of directions.
WITHDRAWN: Revealing the surface treatment technique and degradation characteristics of textiles from a set of thangkas of the mid-Qing Dynasty (1776-1839 CE)
Authors: Zhu Zhanyun , Wang Jia , Zhong Yijia , Ma Qinglin , Li Zhimin , Cao Jing 
Published: February 2023

Abstract
In this paper, X-ray microtomography, scanning electron microscopy - energy dispersive spectroscopy, second harmonic generation imaging microscopy, Fourier transform infrared spectroscopy, and X-ray diffractometry etc. were combined for the scientific examination of the surface treatment technique and degradation characteristics of hemp textiles from six mid-Qing thangkas. The results showed that kaolinite was added as a filler in the production of the canvas, and the fiber morphology and molecular structure of the six hemp thangka textiles were disparate from those of fresh hemp. Specifically, the canvas fiber surfaces were rough, with twisting, deformation, fracture, and decreased cellulose crystallinity indexes. Those results indicated that all six thangkas had undergone significant degradation, which was relatively severe in samples TK16 and TK18. By complimentary evidence derived from the above analyses, the information on the production technique and degradation degree of this group of precious thangkas were obtained, thus enabling the accurate assessment of the preservation status of the thangkas, and providing a scientific basis for its conservation and restoration.
The analyses of the relation between osteoporosis and coffee/green tea/soda drink intake: A cross-sectional study using KoGES HEXA data
Authors: Choi Hyo Geun , Kim So Young , Lee Suk Woo 
Published: February 2023

AbstractBackground:The purpose of this study was to investigate the association between coffee, green tea, and soda drink intake and the risk of osteoporosis using data from the Korean Genome and Epidemiology Study (KoGES).Methodology:Data for patients with osteoporosis (n=11,484) and controls (n=156,320) were extracted using a national cohort study with KoGES Health Examiner (HEXA) data of participants over 40 years of age. We then analyzed the history of coffee/green tea/soda drink intake frequency at baseline from 2004 to 2013 and follow-up data from 2012 to 2016.Results:Regardless of the frequency of coffee intake, coffee intake showed lower odds ratios (ORs) for osteoporosis compared to the non-intake group, but not green tea and soda drink intake did not. According to the subgroup analysis by age and sex, coffee intake showed a lower ORs for osteoporosis in the both group of women age < 53 years of age and ≥ 53 years of age regardless of the frequency of coffee intake.Conclusion:The conclusion of this study was that coffee intake was associated with lower incidence of osteoporosis in Korean women over 40 years of age, regardless of age.
Prediction of pathologic complete response after single-dose MR-guided partial breast irradiation in low-risk breast cancer patients: the ABLATIVE-2 trial - a study protocol
Authors: Civil Yasmin A. , Oei Arlene L. , Duvivier Katya M. , Bijker Nina , Meijnen Philip , Donkers Lorraine , Verheijen Sonja , Kesteren Zdenko van , Palacios Miguel A. , Schijf Laura J. , Barbé Ellis , Konings Inge R.H.M. , Oordt C. Willemien Menke-van der Houven van , Westhoff Paulien G. , Meijer Hanneke J.M. , Diepenhorst Gwen M.P. , Thijssen Victor , Mouliere Florent , Slotman Berend J. , Velde Susanne van der , Bongard H.J.G. Desirée van den 
Published: February 2023

Abstract
BACKGROUND: Partial breast irradiation (PBI) is standard of care in low-risk breast cancer patients after breast-conserving surgery (BCS). Pre-operative PBI can result in tumor downstaging and more precise target definition possibly resulting in less treatment-related toxicity. This study aims to assess the pathologic complete response (pCR) rate one year after MR-guided single-dose pre-operative PBI in low-risk breast cancer patients.
METHODS: The ABLATIVE-2 trial is a multicenter prospective single-arm trial using single-dose ablative PBI in low-risk breast cancer patients. Patients ≥ 50 years with non-lobular invasive breast cancer ≤ 2 cm, grade 1 or 2, estrogen receptor-positive, HER2-negative, and tumor-negative sentinel node procedure are eligible. A total of 100 patients will be enrolled. PBI treatment planning will be performed using a radiotherapy planning CT and -MRI in treatment position. The treatment delivery will take place on a conventional or MR-guided linear accelerator. The prescribed radiotherapy dose is a single dose of 20 Gy to the tumor, and 15 Gy to the 2 cm of breast tissue surrounding the tumor. Follow-up MRIs, scheduled at baseline, 2 weeks, 3, 6, 9, and 12 months after PBI, are combined with liquid biopsies to identify biomarkers for pCR prediction. BCS will be performed 12 months after radiotherapy or after 6 months, if MRI does not show a radiologic complete response. The primary endpoint is the pCR rate after PBI. Secondary endpoints are radiologic response, toxicity, quality of life, cosmetic outcome, patient distress, oncological outcomes, and the evaluation of biomarkers in liquid biopsies and tumor tissue. Patients will be followed up to 10 years after radiation therapy.
DISCUSSION: This trial will investigate the pathological tumor response after pre-operative single-dose PBI after 12 months in patients with low-risk breast cancer. In comparison with previous trial outcomes, a longer interval between PBI and BCS of 12 months is expected to increase the pCR rate of 42% after 6-8 months. In addition, response monitoring using MRI and biomarkers will help to predict pCR. Accurate pCR prediction will allow omission of surgery in future patients.
TRIAL REGISTRATION: The trial was registered prospectively on April 28th 2022 at clinicaltrials.gov (NCT05350722).