bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitrogen-doped hierarchical porous carbons derived from biomass for oxygen reduction reaction
Authors: Wang Min , Chen Yao , Zhao Shunsheng , Zhao Cenkai , Wang Guanxiong , Wu Mingbo 
Published: June 2023

Nowadays biomass has become important sources for the synthesis of different carbon nanomaterials due to their low cost, easy accessibility, large quantity, and rapid regeneration properties. Although researchers have made great effort to convert different biomass into carbons for oxygen reduction reaction (ORR), few of these materials demonstrated good electrocatalytical performance in acidic medium. In this work, fresh daikon was selected as the precursor to synthesize three dimensional (3D) nitrogen doped carbons with hierarchical porous architecture by simple annealing treatment and NH3 activation. The daikon-derived material Daikon-NH3-900 exhibits excellent electrocatalytical performance towards oxygen reduction reaction in both alkaline and acidic medium. Besides, it also shows good durability, CO and methanol tolerance in different electrolytes. Daikon-NH3-900 was further applied as the cathode catalyst for proton exchange membrane (PEM) fuel cell and shows promising performance with a peak power density up to 245 W/g.
Clinical application value of metagenomic second-generation sequencing technology in hematologic diseases with and without transplantation
Authors: Zhang Xia , Wang Fang , Yu Jifeng , Jiang Zhongxing 
Published: June 2023

IntroductionHematological patients are at risk of infections. It is unknown whether the pathogenic microbial spectrum differs between HSCT and non-HSCT patients, and whether metagenomic next-generation sequencing (mNGS) of peripheral blood can be used as a substitute test specimen such as alveolar lavage.MethodsA retrospective study was conducted to evaluate the clinical application value of mNGS in hematological patients with and without HSCT.ResultsViruses were prevalent pathogens in both non-HSCT (44%) and HSCT (45%) patients, chiefly human cytomegalovirus and Epstein–Barr virus. In non-HSCT patients, Gram-negative bacilli accounted for 33% (predominantly Klebsiella pneumonia), and Gram-positive cocci accounted for 7% (predominantly Enterococcus faecium) of pathogens. However, in HSCT patients, Gram-negative bacilli accounted for 13% (predominantly Stenotrophomonas maltophilia), and Gram-positive cocci accounted for 24% (predominantly Streptococcus pneumonia) of pathogens. Mucor was the most common fungu s in two groups. The positive rate of pathogens by mNGS was 85.82%, higher than conventional detection (20.47%, P &lt; 0.05). Mixed infection accounted for 67.00%, among which the mixed infection of bacteria and virus (25.99%) was the most common. 78 cases had pulmonary infection, the positive rate of traditional laboratory tests was 42.31% (33/78), and of mNGS in peripheral blood was 73.08% (57/78), showing a statistical difference (P = 0.000). The non-HSCT patients had a higher frequency of Klebsiella pneumonia (OR=0.777, 95% CI, 0.697-0.866, P = 0.01) and Torque teno virus (OR=0.883, 95% CI, 0.820-0.950, P = 0.031) infections than HSCT patients, while the rates of Streptococcus pneumonia (OR=12.828, 95% CI, 1.378-119.367, P = 0.016), Candida pseudosmooth (OR=1.100, 95% CI, 0.987-1.225, P = 0.016), human betaherpesvirus 6B (OR=6.345, 95% CI, 1.105-36.437, P = 0.039) and human polyomavirus 1 (OR=1.100, 95% CI, 0.987-1.225, P = 0.016) infections were lower. Leishmania could be detected by mNGS.ConclusionmNGS of peripheral blood can be used as a substitute test method for hematological patients with pulmonary infection, the detection rate of mixed infections by mNGS was high, and mNGS has high clinical recognition rate and sensitivity in pathogen detection, and provides a basis for guiding the anti-infective treatment in hematological diseases with symptoms such as fever.
Factors associated with persistent positive in HBV DNA level in patients with chronic Hepatitis B receiving entecavir treatment
Authors: Li Jun , Dong Xiao-Qin , Cao Li-Hua , Zhang Zhan-Qing , Zhao Wei-Feng , Shang Qing-Hua , Zhang Da-Zhi , Ma An-Lin , Xie Qing , Gui Hong-Lian , Zhang Guo , Liu Ying-Xia , Shang Jia , Xie Shi-Bin , Liu Yi-Qi , Zhang Chi , Wang Gui-Qiang , Zhao Hong ,   China HepB Related Fibrosis Assessment Research Group
Published: June 2023

IntroductionThe clinical significance of persistent positive in Hepatitis B Virus (HBV) DNA level in patients receiving antiviral therapy is not well known. We investigated factors associated with persistent viremia (PV) in patients with chronic hepatitis B (CHB) given 78-week entecavir.MethodsA total of 394 treatment-naïve CHB patients who had undergone liver biopsy at baseline and week 78 of treatment were analyzed in this prospective multicentre study. We identified patients with PV (above the lower limit of quantification, 20 IU/ml) after 78 weeks of entecavir therapy. Stepwise, forward, multivariate regression analyses of specified baseline parameters were apllied to identify factors associated with PV. Futhermore, we assessed the incidence of hepatocellular carcinoma (HCC) in all patients using models of the risk of HCC development.ResultsOf the 394 patients, 90 (22.8%) still with PV after 78-week antiviral treatment. Factors associated significantly with PV (vs complete virological response, CVR) were HBV DNA level ≥8 log10 IU/mL (OR, 3.727; 95% CI, 1.851-7.505; P &lt; 0.001), Anti-HBc level &lt; 3 log10 IU/mL (OR, 2.384; 95% CI, 1.223-4.645; P=0.011), and HBeAg seropositivity (OR, 2.871; 95% CI, 1.563-5.272; P &lt; 0.001). Patients with PV were less likely to have fibrosis progression and HCC development than those with the CVR. Of the 11 HBeAg-positive patients with HBV DNA level ≥8 log10 IU/mL and Anti-HBc level &lt; 3 log10 IU/mL at baseline, 9 (81.8%) had persistent positivity in HBV DNA level and 0 had fibrosis progression at week 78 of treatment.DiscussionIn conclusion, HBV DNA level ≥8 log10 IU/mL, Anti-HBc level &lt; 3 log10 IU/mL and HBeAg seropositivity at baseline contribute to PV in patients with CHB receiving 78-week antiviral treatment. In addition, the rate of fibrosis progression and the risk of HCC development in patients with PV were kept low. The complete protocol for the clinical trial has been registered at clinicaltrials.gov (NCT01962155 and NCT03568578).
Use of a new non-contrast-enhanced BOOST cardiac MR sequence before electrical cardioversion or ablation of atrial fibrillation—a pilot study
Authors: Orbán Gábor , Dohy Zsófia , Suhai Ferenc Imre , Nagy Anikó Ilona , Salló Zoltán , Boga Márton , Kiss Máté , Kunze Karl , Neji Radhouene , Botnar Rene , Prieto Claudia , Gellér László , Merkely Béla , Vágó Hajnalka , Szegedi Nándor 
Published: June 2023

IntroductionLeft atrial appendage (LAA) thrombus is the most common source of embolization in atrial fibrillation (AF). Transesophageal echocardiography (TEE) is the gold standard method for LAA thrombus exclusion. Our pilot study aimed to compare the efficacy of a new non-contrast-enhanced cardiac magnetic resonance (CMR) sequence (BOOST) with TEE for the detection of LAA thrombus and to evaluate the usefulness of BOOST images for planning radiofrequency catheter ablation (RFCA) compared with left atrial (LA) contrast-enhanced computed tomography (CT). We also attempted to assess the patients' subjective experiences with TEE and CMR.MethodsPatients with AF undergoing either electrical cardioversion or RFCA were enrolled. Participants underwent pre-procedural TEE and CMR scans to evaluate LAA thrombus status and pulmonary vein anatomy. Patient experiences with TEE and CMR were assessed using a questionnaire developed by our team. Some patients scheduled for RFCA also had pre-procedural LA contrast-enhanced CT. In such cases, the operating physician was asked to subjectively define the quality of the CT and CMR scan on a scale of 1–10 (1 = worst, 10 = best) and comment on CMR's usefulness in RFCA planning.ResultsSeventy-one patients were enrolled. In 94.4%, both TEE and CMR excluded, and in 1 patient, both modalities reported the presence of LAA thrombus. In 1 patient, TEE was inconclusive, but CMR excluded LAA thrombus. In 2 patients, CMR could not exclude the presence of thrombus, but in 1 of those cases, TEE was also indecisive. During TEE, 67%, during CMR, only 1.9% of patients reported pain (p &lt; 0.0001), and 89% would prefer CMR in case of a repeat examination. The quality of the left atrial contrast-enhanced CT scans was better compared with the image quality of the CMR BOOST sequence [8 (7–9) vs. 6 (5–7), p &lt; 0.0001]. Still, the CMR images were useful for procedural planning in 91% of cases.ConclusionThe new CMR BOOST sequence provides appropriate image quality for ablation planning. The sequence might be useful for excluding larger LAA thrombi; however, its accuracy in detecting smaller thrombi is limited. Most patients preferred CMR over TEE in this indication.
Seismic velocity structure of the crust and uppermost mantle beneath the Tien Shan and its adjacent areas
Authors: Zhang Shengzhong , Zhang Bing , Zhou Ming , Ouyang Longbin 
Published: June 2023

The Tien Shan and its adjacent areas have been a prime place to understand the process of continental collision, the mechanism of mountain building and the interaction of tectonic blocks. In this study, we collect seismic data recorded by 74 broad-band stations from the China Provincial Digital Seismic Networks and the Regional Kyrgyzstan and Kazakhstan Networks between January, 2007 and September, 2009. A joint inversion technique that combines three types of datasets (receiver functions, phase velocities of Rayleigh wave measured from both ambient noise and teleseismic earthquake data) is applied to image the crustal and upper mantle structure beneath the Tien Shan and its adjacent areas. The average crustal thickness in the study area is about 50 km, however, the Moho depth extends to ∼70 km beneath the Kyrgyz Platform near the southwestern Tien Shan. Our velocity models show a good correlation with subsurface geological features at shallow depths: low velocities are predominantly observed beneath the basins due to thick sedimentary layer, whereas high velocities are mainly distributed beneath the mountain ranges due to crystalline basement rocks. In the upper mantle a low velocity zone is obviously observed beneath the western Tien Shan. Both the crust thickness and S wave velocity structure of the Tien Shan and its adjacent regions display obvious horizontal and vertical heterogeneities from west to east, which suggests that the far-field effects of the collision between Eurasian plate and Indian plate plays an important role in the tectonic activity of the Tien Shan. The apparent velocity heterogeneities beneath the northern Tarim Basin may indicate that the Tarim Basin may have been eroded and damaged by upwelling hot materials from the upper mantle.
Impact of an SLC30A8 loss-of-function variant on the pancreatic distribution of zinc and manganese: laser ablation-ICP-MS and positron emission tomography studies in mice
Authors: Firth George , Georgiadou Eleni , Griffiths Alexander , Amrahli Maral , Kim Jana , Yu Zilin , Hu Ming , Stewart Theodora J. , Leclerc Isabelle , Okamoto Haruka , Gomez Daniel , Blower Philip J. , Rutter Guy A. 
Published: June 2023

IntroductionCommon variants in the SLC30A8 gene, encoding the secretory granule zinc transporter ZnT8 (expressed largely in pancreatic islet alpha and beta cells), are associated with altered risk of type 2 diabetes. Unexpectedly, rare loss-of-function (LoF) variants in the gene, described in heterozygous individuals only, are protective against the disease, even though knockout of the homologous SLC30A8 gene in mice leads to unchanged or impaired glucose tolerance. Here, we aimed to determine how one or two copies of the mutant R138X allele in the mouse SLC30A8 gene impacts the homeostasis of zinc at a whole-body (using non-invasive 62Zn PET imaging to assess the acute dynamics of zinc handling) and tissue/cell level [using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to map the long-term distribution of zinc and manganese in the pancreas].MethodsFollowing intravenous administration of [62Zn]Zn-citrate (~7 MBq, 150 μl) in wild-type (WT), heterozygous (R138X+/−), and homozygous (R138X+/+) mutant mice (14–15 weeks old, n = 4 per genotype), zinc dynamics were measured over 60 min using PET. Histological, islet hormone immunohistochemistry, and elemental analysis with LA-ICP-MS (Zn, Mn, P) were performed on sequential pancreas sections. Bulk Zn and Mn concentration in the pancreas was determined by solution ICP-MS.ResultsOur findings reveal that whereas uptake into organs, assessed using PET imaging of 62Zn, is largely unaffected by the R138X variant, mice homozygous of the mutant allele show a substantial lowering (to 40% of WT) of total islet zinc, as anticipated. In contrast, mice heterozygous for this allele, thus mimicking human carriers of LoF alleles, show markedly increased endocrine and exocrine zinc content (1.6-fold increase for both compared to WT), as measured by LA-ICP-MS. Both endocrine and exocrine manganese contents were also sharply increased in R138X+/− mice, with smaller increases observed in R138X+/+ mice.DiscussionThese data challenge the view that zinc depletion from the beta cell is the likely underlying driver for protection from type 2 diabetes development in carriers of LoF alleles. Instead, they suggest that heterozygous LoF may paradoxically increase pancreatic β-cell zinc and manganese content and impact the levels of these metals in the exocrine pancreas to improve insulin secretion.
Automated CO2 and CH4 monitoring system for continuous estimation of degassing related to hydropower
Authors: Deblois C. P. , Demarty M. , Bilodeau F. , Tremblay A. 
Published: June 2023

Reliable measurement of greenhouse gas emissions from reservoirs is essential for estimating the carbon footprint of the hydropower industry. Among the different emission pathways, degassing downstream of the turbines and spillway is poorly documented mainly because of the safety stakes related to sampling up and downstream the power plants. The alternative being to sample the water from the turbine inside the station, this study aimed to assemble a custom automated CO2 and CH4 monitoring system (SAGES), especially designed for long-term surveys in hydropower facilities, with a special focus on low maintenance requirements. The SAGES combines infrared and laser technologies with a modular programming approach and run with a specifically designed plexiglass equilibration system (PES) that maintain a permanent headspace and avoid clogging by suspended solids. Although the SAGES is based on commercially available devices, it is the first time they are combined and used with the gas equilibrator. To ensure the reliability of the mounting and to control the quality of the readings, the system was tested in laboratory prior to its installation in generating stations. SAGES and PES performances were compared with those of generic devices available on the market although less adapted to the specific deployments targeted. The SAGES gas partial pressure measurements were accurate and linear in the entire range tested: 0 to 5,000 ppm for pCO2 and 0 to 600 and 10,000 ppm for pCH4. Gas PP measurements were comparable to the reference CO2/CH4 sensor and there was no drift during long term deployment. The SAGES/PES installed in 2021 in cascading generating stations of the Romaine complex collected more than 28,000 data points over a 10-month period and required only two maintenances. Results show that the SAGES is a reliable tool that provide long-term CO2 and CH4 dataset in generating stations while requiring minimal energy, care and maintenance. The data collected in turbine water and the recent use of the SAGES in peat land by a collaborative team demonstrate how the SAGES systems can efficiently contribute to the understanding of reservoir carbon cycles.
Immunity following SARS-CoV-2 vaccination in autoimmune neurological disorders treated with rituximab or ocrelizumab
Authors: Nytrova Petra , Stastna Dominika , Tesar Adam , Menkyova Ingrid , Posova Helena , Koprivova Helena , Mikulova Veronika , Hrdy Jiri , Smela Gabriela , Horakova Dana , Rysankova Irena , Doleckova Kristyna , Tyblova Michaela 
Published: June 2023

BackgroundRituximab (RTX) and ocrelizumab (OCR), B cell-depleting therapy targeting CD20 molecules, affect the humoral immune response after vaccination. How these therapies influence T-cell-mediated immune response against SARS-CoV-2 after immunization remains unclear. We aimed to evaluate the humoral and cellular immune response to the COVID-19 vaccine in a cohort of patients with multiple sclerosis (MS), neuromyelitis optica spectrum disorders (NMOSD), and myasthenia gravis (MG).MethodsPatients with MS (83), NMOSD (19), or MG (7) undergoing RTX (n=47) or OCR (n=62) treatment were vaccinated twice with the mRNA BNT162b2 vaccine. Antibodies were quantified using the SARS-CoV-2 IgG chemiluminescence immunoassay, targeting the spike protein. SARS-CoV-2-specific T cell responses were quantified by interferon γ release assays (IGRA). The responses were evaluated at two different time points (4-8 weeks and 16-20 weeks following the 2nd dose of the vaccine). Immunocompetent vaccinated individuals (n=41) were included as controls.ResultsAlmost all immunocompetent controls developed antibodies against the SARS-CoV-2 trimeric spike protein, but only 34.09% of the patients, without a COVID-19 history and undergoing anti-CD20 treatment (via RTX or OCR), seroconverted. This antibody response was higher in patients with intervals of longer than 3 weeks between vaccinations. The duration of therapy was significantly shorter in seroconverted patients (median 24 months), than in the non-seroconverted group. There was no correlation between circulating B cells and the levels of antibodies. Even patients with a low proportion of circulating CD19+ B cells (&lt;1%, 71 patients) had detectable SARS-CoV-2 specific antibody responses. SARS-CoV-2 specific T cell response measured by released interferon γ was detected in 94.39% of the patients, independently of a humoral immune response.ConclusionThe majority of MS, MG, and NMOSD patients developed a SARS-CoV-2-specific T cell response. The data suggest that vaccination can induce SARS-CoV-2-specific antibodies in a portion of anti-CD20 treated patients. The seroconversion rate was higher in OCR-treated patients compared to those on RTX. The response represented by levels of antibodies was better in individuals, with intervals of longer than 3 weeks between vaccinations.
Protection against tuberculosis achieved by dissolving microneedle patches loaded with live Mycobacterium paragordonae in a BCG prime-boost strategy
Authors: Lee Mi-Hyun , Seo Hyejun , Lee Moon-Su , Kim Byoung Jun , Kim Hye Lin , Lee Du Hyung , Oh Jaehun , Shin Ju Yeop , Jin Ju Young , Jeong Do Hyeon , Kim Bum-Joon 
Published: June 2023

IntroductionSkin vaccination using dissolving microneedle patch (MNP) technology for transdermal delivery is a promising vaccine delivery strategy to overcome the limitations of the existing vaccine administration strategies using syringes. To improve the traditional microneedle mold fabrication technique, we introduced droplet extension (DEN) to reduce drug loss. Tuberculosis remains a major public health problem worldwide, and BCG revaccination had failed to increase the protective efficacy against tuberculosis. We developed an MNP with live Mycobacterium paragordonae (Mpg) (Mpg-MNP) as a candidate of tuberculosis booster vaccine in a heterologous prime-boost strategy to increase the BCG vaccine efficacy.Materials and methodsThe MNPs were fabricated by the DEN method on a polyvinyl alcohol mask film and hydrocolloid-adhesive sheet with microneedles composed of a mixture of mycobacteria and hyaluronic acid. We assessed the transdermal delivery efficiency by comparing the activation of the dermal immune system with that of subcutaneous injection. A BCG prime Mpg-MNP boost regimen was administered to a mouse model to evaluate the protective efficacy against M. tuberculosis.ResultsWe demonstrated the successful transdermal delivery achieved by Mpg-MNP compared with that observed with BCG-MNP or subcutaneous vaccination via an increased abundance of MHCII-expressing Langerin+ cells within the dermis that could migrate into draining lymph nodes to induce T-cell activation. In a BCG prime-boost regimen, Mpg-MNP was more protective than BCG-only immunization or BCG-MNP boost, resulting in a lower bacterial burden in the lungs of mice infected with virulent M. tuberculosis. Mpg-MNP-boosted mice showed higher serum levels of IgG than BCG-MNP-boosted mice. Furthermore, Ag85B-specific T-cells were activated after BCG priming and Mpg-MNP boost, indicating increased production of Th1-related cytokines in response to M. tuberculosis challenge, which is correlated with enhanced protective efficacy.DiscussionThe MNP fabricated by the DEN method maintained the viability of Mpg and achieved effective release in the dermis. Our data demonstrate a potential application of Mpg-MNP as a booster vaccine to enhance the efficacy of BCG vaccination against M. tuberculosis. This study produced the first MNP loaded with nontuberculous mycobacteria (NTM) to be used as a heterologous booster vaccine with verified protective efficacy against M. tuberculosis.