bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of NGL separation process with maximum quality and energy reduction
Authors: Sabeghi Narjes , Kiani Behrooz , Ghanbari Reza , Ghorbani-Moghadam Khatere 
Published: January 2023

Abstract
There are different methods to separate Natural Gas Liquid (NGL) from natural gas. One of these methods is refrigeration. Temperature reduction occurs in the dew point adjustment part to condense the NGL. The aim of this paper is to present a methodology for optimizing the NGL production process by calculating the best quantity for some set-points (the temperature, pressure, and etc for some vessels or other equipment) and at the same time try to reduce the energy consumption. To do this, we use a hybrid algorithm including a Genetic Algorithm (NSGA II) and Artificial Neural Network system (ANN). Indeed, in this research, we define a multi-objective problem and try to investigate more solutions for finding the best pareto-front. Therefore, it needs more time to evaluate solutions, thus, using an ANN to evaluate the amount of objective functions according to different solutions, is faster than the simulation methods. We solve the defined multi-objective problem by using of NSGAII. In order to design a tool that is a decision-helper for selecting the appropriate set-points, the ability of the ANN algorithm along with multi-objective optimization is evaluated. We implement our proposed algorithm on one of the Iranian chemical factories, knowing as the NGL plant, which separates the Natural Gas Liquid (NGL) from natural gas, as the case study of this article. We show the effectiveness of our proposed algorithm by using the nonparametric statistical Kruskal-Wallis.
SIRT4 enhances the sensitivity of colorectal cancer cells to 5-FU by inhibiting ammonia-mediated autophagy
Authors: Xu Bo , Cai Liangliang , Xiao Hua , Cai Guohao , Sun Pingliang , Huang Guoyu 
Published: January 2023

Abstract
5-Fluorouracil (5-FU) has been a first-line chemotherapy agent for colorectal cancer (CRC). However, the emergence of chemoresistance has affected its use. Our preliminary results indicated that SIRT4 levels were decreased in CRC and associated with poor prognosis. Here, SIRT4 enhances the sensitivity of CRC cells to 5-FU. Mechanistically, SIRT4 inhibits autophagy flow within CRC cells, and inhibiting autophagy using bafilomycin or shATG5 counteracts the elevated sensitivity of CRC cells to 5-FU due to SIRT4. Further, SIRT4 inhibited glutamine metabolism and ammonia production in CRC cells by inhibiting glutamate dehydrogenase (GDH). In contrast, inhibition of glutamine metabolism by BPTES or adding ammonium chloride to the medium counteracted the inhibition of autophagy due to SIRT4. Therefore, inhibiting glutamine metabolism or adding ammonium chloride to the medium counteracted the difference in sensitivity of CRC cells to 5-FU because of SIRT4. Together, the current findings identify a novel role of SIRT4 in autophagy manipulation and demonstrate the molecular mechanism behind SIRT4 in enhancing the sensitivity of colorectal cancer cells to 5-FU, providing a promising strategy to increase the efficacy of 5-FU for the treatment of colorectal cancer.
The influence of drug-eluting beads transarterial chemoembolization on serum levels of soluble programed cell death protein-1 in advanced hepatocellular carcinoma patients
Authors: Ma Xiaochen , Sun Xiangyang , Xie Fubo , Jian Wencheng , Wang Qingliang , Xie Yang , Li Caixia , Kai Zhang 
Published: January 2023

Abstract
Aims: To investigate the implications of soluble programmed cell death protein 1 (sPD-1) in hepatocellular carcinoma (HCC) patients treated with drug-eluting beads transarterial chemoembolization (D-TACE) and to evaluate the potential value of sPD-1 to guide selection of the optimal time to begin combination therapy with D-TACE and immune checkpoint inhibitors (ICIs).
Materials and methods:Forty-four HCC patients suitable for TACE and fifty-five healthy volunteers were enrolled in this study. Three milliliters of peripheral venous blood of patients were collected on 1 day before TACE and 3, 7, and 30 days after TACE respectively for assay of sPD-1 using enzyme-linked immunosorbent assay. The associations of the sPD-1 level with the clinical features, outcomes, and the fluctuation of sPD-1 during the treatment were analyzed.
Results: The initial sPD-1 level of patients was significantly higher than that of the control group. Although the initial level of sPD-1 showed a decreasing trend with the increase of BCLC stage, there were no significant differences among patients with different BCLC stages. The sPD-1 level of 3 days after TACE was significantly lower than the initial level but the level of sPD-1 after 7 days of TACE was similar to that after 3 days of TACE. The sPD-1 level of 30 days after TACE was significantly higher than that of 7 days after TACE. When it came to 30 days after TACE, sPD-1 level nearly elevated to the initial level before TACE. The level of sPD-1 of CR and PD patients was lower than that of PR, SD patients, but the differences were not significant.
Conclusion: The level of sPD-1 was significantly elevated in patients with HCC but further research is necessary to better understand the value of sPD-1 in onset, development, and prognosis of HCC as a potential biomarker. The decreases in sPD-1 after D-TACE suggested that D-TACE could probably reduce immune effector cells as well as weaken immune function, which indicated that the ICIs shouldn’t be administered shortly after D-TACE.
Trial registration:
Chinese Clinical Trial Registry (中国临床试验注册中心), ChiCTR1800018846. Registered 14 Octorbor 2018, http://www.chictr.org.cn/showproj.aspx?proj=31528
Amniotic membrane-derived stromal cells release extracellular vesicles that favor regeneration of dystrophic skeletal muscles
Authors: Sandonà Martina , Esposito Federica , Cargnoni Anna , Silini Antonietta , Romele Pietro , Parolini Ornella , Saccone Valentina 
Published: January 2023

Abstract
Background
Duchenne muscular dystrophy (DMD) is a muscle disease caused by mutations in the dystrophin gene characterized by myofiber fragility and progressive muscle degeneration. The genetic defect results in a reduced number of self-renewing satellite cells (MuSCs) and an impairment of their activation and differentiation which lead to the exhaustion of skeletal muscle regeneration potential and muscle replacement by fibrotic and fatty tissue.
In this study we focused on an unexplored strategy to improve MuSC function and their niche based on the regenerative properties of mesenchymal stromal cells from the amniotic membrane (hAMSCs), multipotent cells that have been recognized to have a role in tissue repair in different disease models.
Methods
The myogenic capacity of the hAMSC secretome (CM-hAMSC) and of their extracellular vesicles (EVs) were tested on human myoblasts, muscle fibers and dystrophic muscle stem cells in terms of ability to favor MuSC activation and differentiation both in vitro and in vivo.
Specifically, the regenerative ability of EV-hAMSC was assessed by histological and molecular analyses after intra-muscular injection in the mouse model of DMD –mdx-.
Results
We demonstrate that CM-hAMSC and EVs isolated thereof are able to directly stimulate the in vitro proliferation and differentiation of human myoblasts and mouse MuSC from dystrophic muscles. Indeed, they significantly increased both nuclei numbers (p<0.0001) and fusion index (p<0.0001). Furthermore, we give evidence that EV-hAMSC provide a direct modulation of the muscle stem cell niche in mdx mice.
Interestingly, local injection of EV-hAMSCs in mdx muscles correlated with an increase in the number of activated Pax7+/Ki67+ MuSCs (p<0.001) and in new fiber formation (p<0.0001). EV-hAMSCs reduced muscle collagen deposition by almost 50% (p<0.01), thus counteracting fibrosis and MuSCs exhaustion, two hallmarks of DMD.
Conclusions
In this study, for the first time, we demonstrate that the hAMSC secretome (CM-hAMSC) and mainly EVs derived thereof are able to promote the muscle regeneration potential supporting proliferation and differentiation of resident muscle stem cells.
This study paves the way to develop a novel regenerative treatment to counteract DMD progression based on the capacity of EV-hAMSC to reduce fibrosis and enhance myogenesis in dystrophic muscles.
SOST gene suppression stimulates osteocyte Wnt/β-catenin signaling to prevent bone resorption and attenuates particle-induced osteolysis
Authors: Jiao Zixue , Chai Hao , Wang Shendong , Sun Chunguang , Huang Qun , Xu Wei 
Published: January 2023

Abstract
Background
The most common cause for prosthetic revision surgery is wear particle-induced periprosthetic osteolysis, which leads to aseptic loosening of the prosthesis. Both SOST gene and its synthetic protein, sclerostin, are hallmarks of osteocytes. According to our previous findings, blocking SOST induces bone formation and protects against bone loss and deformation caused by titanium (Ti) particles by activating the Wnt/β-catenin cascade. Although SOST has been shown to influence osteoblasts, its ability to control wear-particle-induced osteolysis via targeting osteoclasts remains unclear.
Methods
Mice were subjected to development of a cranial osteolysis model. Micro CT, HE staining and TRAP staining were performed to evaluate bone loss in the mouse model. Bone marrow-derived monocyte-macrophages (BMMs) made from the C57BL/6 mice were exposed to the medium of MLO-Y4(co-cultured with Ti particles) to transform them into osteoclasts. Bioinformatics methods were used to predict and validate the interaction among SOST, Wnt/β-catenin, RANKL/OPG, TNF-α, and IL-6.
Results
Local bone density and bone volume improved after SOST inhibition, both the number of lysis pores and the rate of skull erosion decreased. Histological research showed that β-catenin and OPG expression were markedly increased after SOST inhibition, whereas TRAP and RANKL levels were markedly decreased. In-vitro, Ti particle treatment elevated the expression of sclerostin, suppressed the expression of β-catenin, and increased the RANKL/OPG ratio in the MLO-Y4 cell line. TNF-α and IL-6 also elevated after treatment with Ti particles. The expression levels of NFAT-1, CTSK, and TRAP in osteoclasts were significantly increased, and the number of positive cells for TRAP staining was increased. Additionally, the volume of bone resorption increased at the same time. In contrast, when SOST expression was inhibited in the MLO-Y4 cell line, these effects produced by Ti particles were reversed.
Conclusion
All the results strongly show that SOST inhibition triggered the osteocyte Wnt/β-catenin signaling cascade and prevented wear particle-induced osteoclastogenesis, which might reduce periprosthetic osteolysis.
Obstetric and neonatal outcomes: Antiseizure medication profile and seizure types in pregnant women in a vulnerability state, Brazil
Authors: Leite Magnúcia De Lima , Silva José Claudio da , de Oliveira Tales Lyra , Mota-Ortiz Sandra Regina , Almeida Daniel dos Santos , Toporcov Tatiana Natasha 
Published: January 2023

Abstract
Objectives
To describe obstetric and neonatal outcomes, antiseizure medication (ASM) use, and types of seizures in pregnant women with epilepsy (PWWE).
Methods
This retrospective cohort study used data collected from the medical records of high-risk maternity hospitals in Alagoas. PWWE aged < 40 years, with controlled or refractory seizures and pregnant women without epilepsy (control group) between 2008 and 2021 were included in the study.
Results
The obstetric and neonatal outcomes observed in PWWE were pregnancy-related hypertension (PrH), vaginal bleeding, preeclampsia, oligohydramnyos, polyhydramnios, stillbirth, greater likelihood of PrH in those with generalized tonic-clonic seizures (GTCS), maternal ICU admission in those with GTCS and status epilepticus and phenytoin and lamotrigine use. PWWE with GTCS had a higher risk of stillbirth. and premature delivery. PWWE with status epilepticus were treated with lamotrigine. Phenobarbital(PB) with diazepam was commonly used in GTCS and status epilepticus. 14% of the patients did not use antiseizure medication (ASM), 50.2% used monotherapy, and 35.8% used polytherapy. 60.9% of patients used PB and 25.2% used carbamazepine.
Conclusion
This study describes the association between adverse obstetric and neonatal outcomes and severe seizure types in PWWE.
Comparison of "Internet +" CBL and traditional CBL in CPPT teaching mode
Authors: Wang Xinqi , Wang Jiale , Hu Haoyuan , Zhao Jiahui , Wang Songyun 
Published: January 2023

Abstract
Objective To study the difference between "Internet +" case-based learning(CBL) and traditional CBL in the CPPT teaching mode and provide foundation and basis for medical teaching reform.
Methods In August 2022, the fourth-year students of the First Clinical College of a medical university received the "Learning Experience" survey, which was conducted to evaluate the teaching satisfaction and learning gain.
Results Both CBL methods have preponderances and weaknesses. "Internet +" CBL is better in teaching satisfaction, understanding and utilization of knowledge, and can effectively improve students' ability to collect and analyze information and improve students' teamwork ability in terms of learning gain. On the basis of being able to complete the syllabus, traditional CBL reduces the study burden before and after class, provides students with more spare time, but challenges students' consciousness.
Conclusion Under the Clinical Pathophysiology &Therapeutics (CPPT) teaching mode, the teaching satisfaction and learning gain of Internet + "CBL" are superior to traditional CBL in different degrees, but Internet + "CBL" increases the burden on students to a certain extent.
Sustainability Factors of Building Information Modeling (BIM) implementing for Successful Construction Project Managemet Life Cycle in Indonesia
Authors: Latupeirissa Josefine Ernestine , Arrang Hermin 
Published: January 2023

Abstract
Building Information Modeling (BIM) is an important asset in construction projects from conceptual planning, pre-construction to operation stage. While many construction projects in developed countries are gradually implementing BIM, the development of this technology in developing countries is still lagging behind. Some research results state that the application of BIM for certain uses can improve the performance of construction projects, but in Indonesia its use is still very less. Therefore, the purpose of this study is to analyze the factors what can answer the challenges in the sustainability of BIM implementation in construction projects in Indonesia for success in its life cycle.. Data were obtained from 44 respondents who answered the questionnaire questions, are owners, supervisory consultants, and contractors representing construction parties in Indonesia. Empirical analysis using multiple linear regression states that there are five factors which, if sustainably support the application of BIM, will affect the success of construction project management in its life cycle. The ranking of five factors based on the results of the analysis is Understanding and Awareness of the importance of BIM; Establishment of BIM standards, codes, rules, and regulations; Competencies and Skills; Commitment and Consistency; and Monitoring and Evaluation. The development of BIM has resulted in new work processes and new ways to interact in a construction project throughout its life cycle.
Whey protein supplementation improves hypoalbuminemia in critically ill neurological patients: a single-center randomized study
Authors: Tian Fei , Liu Gang , He Hong-Mei , Chen Wei-Bi , Fan Lin-Lin , Cui Li-Li , Zhang Yan 
Published: January 2023

Abstract
Background:Whether whey protein supplementation can reduce the occurrence of hypoproteinemia in patients with acute critically ill neurological diseases on the basis of standard intact EN has not been confirmed by randomized controlled clinical trials.
Objective:We evaluated the effect of whey protein powder on serum albumin in acute critically ill neurological patients.
Methods:Critically ill neurological patients expected to stay longer than 7 days were randomized. For the intact protein formula (IPF) group, only an intact protein formula was given. For the whey protein supplementation formula (WPF) group, whey protein powder was added on the basis of an intact protein formula.
Results: The serum albumin level in the WPF group was higher than that in the IPF group (31.92 ± 4.48 / 29.55 ± 4.31, P = 0.005, 95%CI 0.746 −3.981). There was no significant difference in the complications of enteral nutrition treatment between the two groups (p＞0.05). The serum albumin levels in the WPF group was higher than that in the IPF group for patients 60 years or older and for stroke patients respectively (P＜0.05). Interleukin-6 (OR = -0.023, P = 0.018, 95%CI -0.042 − -0.004) and procalcitonin (OR = -8.331, P = 0.004, 95%CI -13.931 − -2.730) were negatively independently correlated with the serum albumin levels respectively. There was no significant difference in the mRS between the two groups at 14 days and 90 days after onset (p＞0.05).
Conclusion: Whey protein supplementation can improve the severity of hypoproteinemia in critically ill patients with neurological disease, especially in elderly patients and in patients with stroke. At the same time, we need to pay more attention to persistent inflammatory responses and secondary infections. Further clinical studiesat different levels are needed to discover more evidence to help better managethese patients.
URL: https://clinicaltrials.gov/ct2/show/NCT04361357?term=04361357&draw=2&rank=1.
Registration ID: NCT04361357
Quantifying the observed impacts of sea level rise on saltwater intrusion in the Pearl River estuary
Authors: Hu Haiju , Chen Gedun , Lin Rui , Huang Xing , Wei Zhidong , Chen Guoheng 
Published: January 2023

Abstract
The saltwater intrusion occurs in many coastal areas worldwide and has become a serious menace to freshwater quality. In the Pearl River estuary, saltwater intrusion has threatened the water supply for residential, agricultural, and industrial uses in the past decades, and resulted in economic losses. In this study, the observed saltwater intrusion records of 10 years (2012~2021) at 9 stations in Modaomen waterway, the largest inlet of the Pearl River estuary, were compiled and analyzed with the reported sea level heights and river discharge volumes during the same period. Along the Modaomen waterway, the annual saltwater intrusion days have inversely correlated exponential relationship (R2 = 0.97) with distance away from the Pearl River estuary mouth. The results from a multiple linear regression show that the observed annual saltwater intrusion days near the main Pearl River estuary mouth could be quantitatively explained by the combination of river discharge volumes and sea level rise values. Every 1 mm increase of annual sea level rise, would led to an extended saltwater intrusion for 0.410±0.116 day in the same year near the main estuary mouth of the Pearl River. This work presents an example to investigate the sea level rise impacts on saltwater intrusion with annually compiled observed data and from a time-change perspective.
Deep-Learning-Based Neural Network for Design of Dual-Band Coupled-Line Trans-Directional Coupler
Authors: Sallam Tarek , Eldesouki Eman M. , Attiya Ahmed M. 
Published: January 2023

Abstract
A deep-learning-based model to automate the design of dual-band coupled-line trans- directional (CL-TRD) coupler can greatly improve upon the current techniques that rely on complicated analysis. In this paper, we propose a convolutional neural network (CNN), which is a type of deep learning, which can rapidly output the parameters of dual-band directional couplers corresponding to theoretical (ideal) specifications of the electrical parameters through an inverse model. The neural network training data is generated by the use of electromagnetic simulation tool HFSS by varying the geometrical design parameters of the coupler. In order to validate the robustness of the CNN inverse model, it is applied on a 3-dB dual-band CL-TRD coupler operating at 1.2/4 GHz, and compared with a shallow neural network namely, radial basis function neural network (RBFNN). The coupler parameters designed by both neural networks are verified by HFSS. The results reveal that the CNN simulated S-parameters and output ports phase difference are in good agreements with the ideal ones compared to those of RBFNN with more accuracy and speed. The designed coupler is fabricated and measured for verification.
Native bamboo increases biotic resistance to Pinus contorta invasion in temperate forest ecosystems
Authors: Ulloa Javier , Fuentes-Lillo Eduardo , Fuentes-Ramírez Andres , Pauchard Aníbal , García Rafael A. 
Published: January 2023

Abstract
One of the tree species with the greatest invasive potential worldwide is Pinus contorta Douglas ex Loundon, which is characterized by producing a great number of individuals in short periods of time, generating different impacts on the ecosystems it invades. The genus Chusquea is present in a diverse number of ecosystems in South America. In the temperate forests of Chile and Argentina, it forms dense thickets in the undergrowth as well as in open areas. Its rapid growth and vegetative reproduction, with large numbers of individuals, allow the species to dominate and restrict the establishment of other plant species. This study focused on analyzing the effect of the presence of individuals of Chusquea culeou E. Desv. on the establishment of the invasive species Pinus contorta, as well as on other native herbaceous and shrub species in Araucaria-Nothofagus forest. With this aim, we established 204 plots within the Malalcahuello National Reserve (in the Andes of south-central Chile) along a gradient of P. contorta invasion, where the presence or absence of these species was recorded through different percentages of Chusquea cover. The results showed that the probability of presence of P. contorta decreases significantly in areas where Chusquea cover is greater than 25%. Native species also decrease their presence, but to a lesser extent compared to P. contorta. Our results demonstrate the inhibitory effect of Chusquea for the establishment of other plant species, but more markedly for invasive conifers.