bluuebunny/crossref_metadata_2025_split huggingface dataset:
IKK2/NFkB signaling controls lung resident CD8+ T cell memory during influenza infection
Authors: Pritzl Curtis J. , Luera Dezzarae , Knudson Karin M. , Quaney Michael J. , Calcutt Michael J. , Daniels Mark A. , Teixeiro Emma 
Published: July 2023

AbstractCD8+ T cell tissue resident memory (TRM) cells are especially suited to control pathogen spread at mucosal sites. However, their maintenance in lung is short-lived. TCR-dependent NFkB signaling is crucial for T cell memory but how and when NFkB signaling modulates tissue resident and circulating T cell memory during the immune response is unknown. Here, we find that enhancing NFkB signaling in T cells once memory to influenza is established, increases pro-survival Bcl-2 and CD122 levels thus boosting lung CD8+ TRM maintenance. By contrast, enhancing NFkB signals during the contraction phase of the response leads to a defect in CD8+ TRM differentiation without impairing recirculating memory subsets. Specifically, inducible activation of NFkB via constitutive active IKK2 or TNF interferes with TGFβ signaling, resulting in defects of lung CD8+ TRM imprinting molecules CD69, CD103, Runx3 and Eomes. Conversely, inhibiting NFkB signals not only recovers but improves the transcriptional signature and generation of lung CD8+ TRM. Thus, NFkB signaling is a critical regulator of tissue resident memory, whose levels can be tuned at specific times during infection to boost lung CD8+ TRM.
Cultural differences in joint attention and engagement in mutual gaze with a robot face
Authors: Marchesi Serena , Abubshait Abdulaziz , Kompatsiari Kyveli , Wu Yan , Wykowska Agnieszka 
Published: July 2023

AbstractJoint attention is a pivotal mechanism underlying human ability to interact with one another. The fundamental nature of joint attention in the context of social cognition has led researchers to develop tasks that address this mechanism and operationalize it in a laboratory setting, in the form of a gaze cueing paradigm. In the present study, we addressed the question of whether engaging in joint attention with a robot face is culture-specific. We adapted a classical gaze-cueing paradigm such that a robot avatar cued participants’ gaze subsequent to either engaging participants in eye contact or not. Our critical question of interest was whether the gaze cueing effect (GCE) is stable across different cultures, especially if cognitive resources to exert top-down control are reduced. To achieve the latter, we introduced a mathematical stress task orthogonally to the gaze cueing protocol. Results showed larger GCE in the Singapore sample, relative to the Italian sample, independent of gaze type (eye contact vs. no eye contact) or amount of experienced stress, which translates to available cognitive resources. Moreover, since after each block, participants rated how engaged they felt with the robot avatar during the task, we observed that Italian participants rated as more engaging the avatar during the eye contact blocks, relative to no eye contact while Singaporean participants did not show any difference in engagement relative to the gaze. We discuss the results in terms of cultural differences in robot-induced joint attention, and engagement in eye contact, as well as the dissociation between implicit and explicit measures related to processing of gaze.
von Willebrand factor-binding protein (vWbp)-activated factor XIII and transglutaminase 2 (TG2) promote cross-linking between FnBPA from Staphylococcus aureus and fibrinogen
Authors: Motta Chiara , Pellegrini Angelica , Camaione Stefano , Geoghegan Joan , Speziale Pietro , Barbieri Giulia , Pietrocola Giampiero 
Published: July 2023

AbstractThe secreted von Willebrand factor-binding protein (vWbp) from Staphylococcus aureus interacts with the coagulation factors prothrombin and fibrinogen (Fbg), leading to the non-proteolytic transglutaminase activation of Factor XIII (FXIII). In this study we found that vWbp-activated FXIII catalyses the incorporation of amino-donor dansylcadaverine into region A of fibronectin-binding protein A (FnBPA). Incubation of Fbg with recombinant region A of S. aureus Fbg-binding proteins FnBPA, FnBPB, ClfA or ClfB in presence of vWbp-activated FXIII resulted in the formation of high molecular heteropolymers with FnBPA only, suggesting a specificity of the cross-linking reaction between fibrin(ogen) and the staphylococcal surface. As previously observed, cross-linking sites were mapped to the α-chain and the N1 subdomain of fibrin(ogen) and region A of FnBPA, respectively. Comparable results were obtained when tissue tranglutaminase-2 (TG2) was tested for cross-linking of FnBPA and Fbg. Of note, FnBPA-mediated covalent cross-linking promoted by vWbp-activated FXIII was also observed when bacteria were allowed to attach to fibrin(ogen). Together these findings suggest a novel pathogenetic mechanism by which the transglutaminase action of FXIII and/or TG2 contributes to entrapment and persistence of S. aureus in blood and host tissues.
FASDetect as a machine learning-based screening app for FASD in youth with ADHD
Authors: Ehrig Lukas , Wagner Ann-Christin , Wolter Heike , Correll Christoph U. , Geisel Olga , Konigorski Stefan 
Published: July 2023

AbstractFetal alcohol-spectrum disorder (FASD) is underdiagnosed and often misdiagnosed as attention-deficit/hyperactivity disorder (ADHD). Here, we develop a screening tool for FASD in youth with ADHD symptoms. To develop the prediction model, medical record data from a German University outpatient unit are assessed including 275 patients aged 0–19 years old with FASD with or without ADHD and 170 patients with ADHD without FASD aged 0–19 years old. We train 6 machine learning models based on 13 selected variables and evaluate their performance. Random forest models yield the best prediction models with a cross-validated AUC of 0.92 (95% confidence interval [0.84, 0.99]). Follow-up analyses indicate that a random forest model with 6 variables – body length and head circumference at birth, IQ, socially intrusive behaviour, poor memory and sleep disturbance – yields equivalent predictive accuracy. We implement the prediction model in a web-based app called FASDetect – a user-friendly, clinically scalable FASD risk calculator that is freely available athttps://fasdetect.dhc-lab.hpi.de.
Identifying dysregulated immune cell subsets following volumetric muscle loss with pseudo-time trajectories
Authors: Hymel Lauren A. , Anderson Shannon E. , Turner Thomas C. , York William Y. , Zhang Hongmanlin , Liversage Adrian R. , Lim Hong Seo , Qiu Peng , Mortensen Luke J. , Jang Young C. , Willett Nick J. , Botchwey Edward A. 
Published: July 2023

AbstractVolumetric muscle loss (VML) results in permanent functional deficits and remains a substantial regenerative medicine challenge. A coordinated immune response is crucial for timely myofiber regeneration, however the immune response following VML has yet to be fully characterized. Here, we leveraged dimensionality reduction and pseudo-time analysis techniques to elucidate the cellular players underlying a functional or pathological outcome as a result of subcritical injury or critical VML in the murine quadriceps, respectively. We found that critical VML resulted in a sustained presence of M2-like and CD206hiLy6Chi ‘hybrid’ macrophages whereas subcritical defects resolved these populations. Notably, the retained M2-like macrophages from critical VML injuries presented with aberrant cytokine production which may contribute to fibrogenesis, as indicated by their co-localization with fibroadipogenic progenitors (FAPs) in areas of collagen deposition within the defect. Furthermore, several T cell subpopulations were significantly elevated in critical VML compared to subcritical injuries. These results demonstrate a dysregulated immune response in critical VML that is unable to fully resolve the chronic inflammatory state and transition to a pro-regenerative microenvironment within the first week after injury. These data provide important insights into potential therapeutic strategies which could reduce the immune cell burden and pro-fibrotic signaling characteristic of VML.
3D reconstruction of skin and spatial mapping of immune cell density, vascular distance and effects of sun exposure and aging
Authors: Ghose Soumya , Ju Yingnan , McDonough Elizabeth , Ho Jonhan , Karunamurthy Arivarasan , Chadwick Chrystal , Cho Sanghee , Rose Rachel , Corwin Alex , Surrette Christine , Martinez Jessica , Williams Eric , Sood Anup , Al-Kofahi Yousef , Falo Louis D. , Börner Katy , Ginty Fiona 
Published: July 2023

AbstractMapping the human body at single cell resolution in three dimensions (3D) is important for understanding cellular interactions in context of tissue and organ organization. 2D spatial cell analysis in a single tissue section may be limited by cell numbers and histology. Here we show a workflow for 3D reconstruction of multiplexed sequential tissue sections: MATRICS-A (Multiplexed ImageThree-DReconstruction andIntegratedCellSpatial -Analysis). We demonstrate MATRICS-A in 26 serial sections of fixed skin (stained with 18 biomarkers) from 12 donors aged between 32–72 years. Comparing the 3D reconstructed cellular data with the 2D data, we show significantly shorter distances between immune cells and vascular endothelial cells (56 µm in 3Dvs108 µm in 2D). We also show 10–70% more T cells (total) within 30 µm of a neighboring T helper cell in 3Dvs2D. Distances of p53, DDB2 and Ki67 positive cells to the skin surface were consistent across all ages/sun exposure and largely localized to the lower stratum basale layer of the epidermis. MATRICS-A provides a framework for analysis of 3D spatial cell relationships in healthy and aging organs and could be further extended to diseased organs.
Some bounds on spectral radius of signless Laplacian matrix of k-graphs
Authors: Zhang Junhao , Zhu Zhongxun 
Published: July 2023

For a k-graph H = (V(H), E(H)), let B(H) be its incidence matrix, and Q(H) = B(H)B(H)T be its signless Laplacian matrix, and this name comes from the fact that Q(H) is exactly the well-known signless Laplacian matrix for 2-graph. Define the largest eigenvalue ρ(H) of Q(H) as the spectral radius of H. In this paper, we give some lower and upper bounds on ρ(H) using some structural parameters (such as independent number, maximum degree, minimum degree, diameter, and so on) of H, which extended or improved some known results.
Medical historical notes on myoma treatment by uterine artery embolization on the occasion of its introduction 30 years ago
Authors: Psilopatis Iason , Fleckenstein Florian Nima , Gebauer Bernhard , David Matthias 
Published: July 2023

Background Since its development, uterine artery embolization (UAE) for the treatment of symptomatic fibroids has become an established minimally invasive alternative to surgical myoma treatment. Currently, more than 25,000 myoma patients worldwide are likely to be treated with UAE annually.
Method Thirty years ago, Jacques-Henri Ravina (b. 1930) and his Paris team first performed this therapy as a “substitute” for gynecologic surgery. We contacted him as part of the preparation of the present review. In addition, we performed a literature search with the aim of summarizing the current literature and data in addition to a historical account of the development of UAE.
Results and Conclusion On the occasion of this anniversary, we would like to recall the interdisciplinary roots and some facets of the history of the development of this relatively young myoma treatment method.
Key points
Citation Format
Operative Time Predicts Long-Term Abdominal Morbidity and Complication Requiring Treatment after DIEP Flap Breast Reconstruction
Authors: Wen Y. Edward , Steppe Cyrus , Teotia Sumeet S. , Haddock Nicholas T. 
Published: July 2023

Abstract
Background The relationship between skin-to-skin operative time and long-term complications, as well as complications requiring treatment, after deep inferior epigastric perforator (DIEP) flap breast reconstructions has not been thoroughly investigated. The study objective was to evaluate if operative time would independently prognosticate the type and number of treated complications, as well as long-term abdominal morbidity.
Methods Patients who underwent bilateral DIEP flaps from 2010 to 2021 by two senior surgeons with standardized surgical and postoperative protocols were retrospectively reviewed. Inclusion required at least 1 year of postoperative follow-up. Extensive multivariable regression analyses were utilized to adjust for potential confounders, including measures of intraoperative complexity.
Results Three hundred thirty-five patients were entered into multivariable analyses. After risk adjustment, there was a significant increase in the risk of all treated complications per hour. Each hour of operation increased risk of complications requiring extended hospital stay for treatment by 25%, flap-site complications requiring readmission treatment by 24%, and flap-site complications requiring extended hospital stay for treatment by 26% (all p < 0.0001). Long-term abdominal morbidity (abdominal bulge) increased by 25% per hour of operative time (p < 0.0001). The number of complications requiring treatment, abdominal donor-site complications, and complications requiring extended hospital stay for treatment had statistically significant linear relationships with the duration of surgery (all p < 0.05).
Conclusion Operative time predicts long-term abdominal morbidity and complications requiring treatment, especially impacting the odds of developing abdominal bulges and complications requiring extended hospital stay for treatment or readmission treatment. This study emphasizes the importance of reducing operative time to improve DIEP flap breast reconstruction outcomes.
Designing Zr12O12 nanocage for the delivery of anticancer drugs: a theoretical study
Authors: Cui Yao-Fei , Zhang Li , Wang Wen-Lu , Yang Jian-Feng , Chen Jing-Hua , Sun Wei-Ming 
Published: July 2023

Abstract
A novel superatom-assembled Zr12O12 nanocage has been theoretically designed and characterized to investigate its potential application as a novel delivery carrier for 5-fluorouracil (5-Fu), mercaptopurine (MP), and thioguanine (TG) via density functional theory calculations in this work. The designed Zr12O12 nanocage possesses high stability in view of its large binding energy (E
b), atomic cohesion energy (E
col), and highest occupied molecular orbital-lowest unoccupied molecular orbital gap. Our results reveal that Zr12O12 tends to bind with 5-Fu via a single Zr–O bond and combine with MP and TG through multidentate chelate modes with the adsorption energies of −22.27 to −55.19 kcal mol−1. The Wiberg bond index, atoms in molecules theory, and localized molecular orbitals analyses demonstrate that all the newly formed linkage bonds between Zr12O12 and drugs are polar covalent bonds. In particular, among these studied drugs, the recovery time for the near-infrared light-triggered release of TG drug from Zr12O12 surface is the shortest, indicating that Zr12O12 can serve as an excellent candidate for the delivery of TG. This study not only offers a new member to enrich the inorganic nanocage family but also provides a potential carrier for the delivery of anticancer drugs.
Electronic and optical properties of the hydrogen boride sheet from the many-body perturbation theory
Authors: Thi Ta Luong , Morikawa Yoshitada , Hamada Ikutaro 
Published: July 2023

Abstract
We study the electronic and optical properties of the hydrogen boride sheet by using the many-body perturbation theory with the perturbative GW (

G
0

W
0

) approximation. It was found that the hydrogen boride sheet shows a semimetallic electronic structure, supporting the previous theoretical study based on the semilocal density functional theory calculations. It was also found that the optical spectrum calculated based on the quasiparticle energies agrees well with the experiments. This work suggests that

G
0

W
0

approximation may be useful for predicting precise electronic and optical properties of the hydrogen boride sheet and its derivatives.
Rashba spin-splitting in Janus SnXY/WXY (X, Y = S, Se, Te; X ≠ Y) heterostructures
Authors: Bhat Bhagyashri Devaru 
Published: July 2023

Abstract
Janus transition metal dichalcogenide monolayers have shown a lack of mirror symmetry perpendicular to the 2D plane. The breaking of out-of-plane symmetry, along with the spin–orbit coupling, induces Rashba spin-splitting (RSS) in these materials. In this work, RSS in Janus tin dichalcogenide monolayers are studied. In addition, the heterostructures (HSs) of Janus SnXY and WXY (X, Y = S, Se, Te; X ≠ Y) monolayers are discussed. A RSS energy of about 43 meV, more significant than the room temperature energy, is observed in the Janus SnSSe/WSSe HS. The consequences of vertical strain on the semiconducting HS are examined. Compressive vertical strain enhances and tensile strain reduces, the spin-splitting. For the compressive strain of 10.4%, Janus SnSSe/WSSe HS remains semiconductor with only Rashba bands surrounding near the Fermi level. Enhanced Rashba parameter of about 0.96 eV Å and splitting energy of about 72 meV are observed. These findings confirm that Janus SnSSe/WSSe HS is a productive Rashba material for spintronic device applications.
Application of equivalent source intensity density interpolation in near-field acoustic holography
Authors: He Huihui , Gao Yuan , Yang Boquan , Shi Shengguo 
Published: July 2023

Abstract
The spatial resolution of near-field acoustic holography based on the equivalent source method (ESM) is closely related to the number of measurement points, the higher the number of measurement points, the higher the resolution. However, the number of measurement points in the actual measurement cannot be increased infinitely. To solve the contradiction between the resolution and the number of measurement points, this paper proposes an equivalent source density interpolation method (ESDIM). First, the equivalent source intensity is obtained using the sound pressure measured by the array element and the Green function, and the equivalent source intensity density is obtained based on the equivalent source intensity and grid area. Second, the Hermite interpolation function was used to obtain the interpolated equivalent source intensity density. However, as the number of interpolated grids increased, the resolution, computation, and running time of ESDIM increased, and the number of subdivided grids per unit grid was 9–25 in this study. Finally, the sound field was reconstructed based on the obtained interpolated equivalent source intensity and Green transfer function, and the reconstruction accuracies of ESDIM and ESM were compared and analyzed. The simulation and experimental data processing results showed that the resolution of the equivalent source intensity density interpolation method was higher than that of the ESM.
pH sensor using aniline blue and functionalized nylon for monitoring the final stage of concrete carbonation
Authors: Jeong Hoseong , Jung Byung Jun , Kim Jae Hyun , Seo Soo-Yeon , Yun Hyun-do , Kim Kang Su 
Published: July 2023

Abstract
The construction industry is one of the most significant contributors to global warming, with 5% of the world’s CO2 emissions coming from the manufacturing process of cement. Therefore, to slow the rate of global warming, efforts are needed to increase the lifespan of infrastructure via structural health monitoring and timely repair. Carbonation is often cited as a significant cause of the deterioration of concrete structures and is measured using a destructive testing in the current structural code. However, since this method is time-consuming, costly, and labor-intensive, the development of pH sensors has been required for monitoring carbonation. Most pH sensors developed so far were manufactured using a physical entrapment method, so they are vulnerable to leaching and cannot guarantee long-term durability. A few pH sensors ensuring durability through covalent immobilization have also been proposed, but they pose limitations since most of them can only monitor the early stage of carbonation. Therefore, this study sought to develop a pH sensor that can be used to measure the final stage of carbonation. The sensor was manufactured via functionalization of nylon using formaldehyde and chloroacetyl chloride and then covalent bonding with aniline blue. The sensor showed a significant color change in the pH ranging from 5 to 10 and exhibited sufficient response within 30 min and reversibility. In addition, the sensor was stable even in high pH environments such as concrete, and its applicability was verified inside the concrete.
Perovskite single crystals: physical properties and optoelectronic applications
Authors: Li Chen , Sun Haoxuan , Gan Shan , Dou Da , Li Liang 
Published: July 2023

Abstract
Single crystal is the most advantageous of the crystalline states of halide perovskites. It displays better optical and electrical capabilities than polycrystalline films and microcrystals due to their inherent structural advantages, such as free grain boundaries, long-range ordered structure, and high orientation. Single-crystal perovskite materials can theoretically enable optoelectronic devices with higher performance and stronger stability. In this review, the intrinsic physical properties of perovskite single crystals are analyzed. The most recent advances in single-crystal optoelectronic devices are reviewed, and the design principles of the devices under different application conditions are revealed. It provides potential solutions for remaining challenges, and it is expected to accelerate the development of perovskite based optoelectronic devices.
New Horizons in cellular senescence for clinicians
Authors: Witham Miles D , Granic Antoneta , Miwa Satomi , Passos Joao F , Richardson Gavin D , Sayer Avan A 
Published: July 2023

Abstract
Cellular senescence has emerged as a fundamental biological mechanism underpinning the ageing process and has been implicated in the pathogenesis of an increasing number of age-related conditions. Cellular senescence is a cell fate originally defined as an irreversible loss of replicative potential although it is now clear that it can be induced by a variety of mechanisms independent of replication and telomere attrition. The drivers include a persistent DNA damage response causing multiple alterations in cellular function. Senescent cells secrete a range of mediators that drive chronic inflammation and can convert other cells to the senescent state—the senescence-associated secretory phenotype. Much research to date has been conducted in animal models, but it is now clear that senescent cells accompany ageing in humans and their presence is an important driver of disease across systems. Proof-of-concept work suggests that preventing or reversing senescence may be a viable strategy to counteract human ageing and age-related disease. Possible interventions include exercise, nutrition and senolytics/senostatic drugs although there are a number of potential limitations to the use of senotherapeutics. These interventions are generally tested for single-organ conditions, but the real power of this approach is the potential to tackle multiple age-related conditions. The litmus test for this exciting new class of therapies, however, will be whether they can improve healthy life expectancy rather than merely extending lifespan. The outcomes measured in clinical studies need to reflect these aims if senotherapeutics are to gain the trust of clinicians, patients and the public.