bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between genetic variants in TREM1, CXCL10, IL4, CXCL8 and TLR7 genes with the occurrence of congenital Zika syndrome and severe microcephaly
Authors: Santos Camilla Natália Oliveira , Magalhães Lucas Sousa , Fonseca Adriana Barbosa de Lima , Bispo Ana Jovina Barreto , Porto Roseane Lima Santos , Alves Juliana Cardoso , dos Santos Cliomar Alves , de Carvalho Jaira Vanessa , da Silva Angela Maria , Teixeira Mauro Martins , de Almeida Roque Pacheco , dos Santos Priscila Lima , de Jesus Amélia Ribeiro 
Published: March 2023

AbstractCongenital Zika syndrome (CZS) is a cluster of malformations induced by Zika virus (ZIKV) infection and the underline mechanisms involved in its occurrence are yet not fully understood. Along with epidemiological and environmental factors, the genetic host factors are suggested as important to the CZS occurrence and development, however, few studies have evaluated this. This study enrolled a total of 245 individuals in a case–control association study compound a cohort of high specific interest constituted by 75 mothers who had delivered CZS infants, their 76 infants, and 47 mothers that had delivered healthy infants, and their 47 infants. Sixteen single-nucleotide polymorphisms on TREM1, CXCL10, IL4, CXCL8, TLR3, TLR7, IFNR1, CXCR1, IL10, CCR2 and CCR5 genes were genotyped to investigate their association as risk factors to CZS. The results show an association between C allele at TREM1 rs2234246 and C allele at IL4 rs224325 in mothers infected with ZIKV during pregnancy, with the increased susceptibility to CZS occurrence in their infants and the SNP CXCL8 rs4073 and the G allele at CXCL10 rs4508917 with presence of CZS microcephaly in the infants. Furthermore, the T allele at CXCL8 rs4073 and TRL7 rs179008 SNPs were associated with the severity of microcephaly in children with CZS. These results suggest that these polymorphisms in genes of innate immune responses addressed here are associated to increased risk of occurrence and severity of CZS in pregnant mothers infected with ZIKV and their CZS infants.
Comparative dosimetric analysis of volumetric modulated arc therapy based craniospinal irradiation plans between Halcyon ring gantry and TrueBeam C-arm linear accelerator
Authors: Sarkar Biplab , Biswal Subhra Snigdha , Shahid Tanweer , Ghosh Tanmoy , Bhattacharya Jibak , De Arundhati , Mukherjee Mukti , Ganesh Tharmarnadar , Cozzi Luca 
Published: March 2023

AbstractThis study evaluates the volumetric modulated arc therapy (VMAT) dosimetric comparison between Halcyon ring gantry and TrueBeam c-arm linear accelerators for craniospinal irradiation (CSI) of the neuro-axis. 25 patients, who received treatment for medulloblastoma and primitive neuro-ectodermal tumors between 2018 and 2021, were planned for VMAT in True Beam (TB), and Halcyon (HAL) linear accelerators using 6 MV unflattened (FFF) photon beams (HALFFF and TBFFF). Dose-volume statistics for the target and organs at risk (OARs) and the total number of monitoring units (MUs) in the treatment plans were compared which included dose received by 95% PTV volume (V95%), volume receiving ≥ 107% dose, homogeneity index (HI), conformity index (PI), MU and dose spillage (D10%, D30%, D50%, D70%, D90%). In all 26 OARs were considered of which five were serial and the remaining were parallel structures. For the former, the dose received by 0.2 cm3, volume = D0.2 cm3) were evaluated and for the latter mean dose were evaluated. Both arms were statistically compared with paired sample t-test with a significant value of ≤ 0.05. 11 patients received treatment with the Halcyon and the rest 14 in the TrueBeam C-arm linear accelerator. Patients in the low- and intermediate-risk category (n = 13) received 23.4 Gy in 13 fractions. The remaining patients were in the high-risk category and received 35 Gy in 21 fractions or 36 Gy in 20 fractions. For HALFFF and TBFFF, PTVV95% were 97.5 ± 0.8% and 97.4 ± 0.9% respectively (p = 0.371) while the V107% were 0.6 ± 0.4% and 0.5 ± 0.5 respectively (p = 0.504). However, the number of monitoring units showed statistical significance (p < 0.001) with values of 1331.9 ± 243.4 MU and 1089 ± 206.7 MU respectively for the HAL and TB plans. The differences in spillage dose were also statistically significant, favouring HAL plans at D30% (p = 0.002), D50% (p < 0.001), D70% (p = 0.039), and D90% (p = 0.01) level except for D10% (p = 0.090). Conformity index also showed statistical significance with PI_HAL = 0.9 ± 0.02 and PI_TB = 0.89 ± 0.03 (p = 0.029). For 10 of the 21 parallel structures, the mean dose differences were statistically significant in favouring of HAL plans. Halcyon based VMAT CSI plans are dosimetrically superior in terms of organ dose, especially for the large organs, and offer lower spillage doses than the TrueBeam plans. Plans generated by both linear accelerators are suitable for the patients’ treatments.
Subset of the periodontal ligament expressed leptin receptor contributes to part of hard tissue-forming cells
Authors: Oka Hirotsugu , Ito Shinichirou , Kawakami Mana , Sasaki Hodaka , Abe Shinichi , Matsunaga Satoru , Morita Sumiharu , Noguchi Taku , Kasahara Norio , Tokuyama Akihide , Kasahara Masataka , Katakura Akira , Yajima Yasutomo , Mizoguchi Toshihide 
Published: March 2023

AbstractThe lineage of periodontal ligament (PDL) stem cells contributes to alveolar bone (AB) and cementum formation, which are essential for tooth-jawbone attachment. Leptin receptor (LepR), a skeletal stem cell marker, is expressed in PDL; however, the stem cell capacity of LepR+ PDL cells remains unclear. We used a Cre/LoxP-based approach and detected LepR-cre-labeled cells in the perivascular around the root apex; their number increased with age. In the juvenile stage, LepR+ PDL cells differentiated into AB-embedded osteocytes rather than cementocytes, but their contribution to both increased with age. The frequency of LepR+ PDL cell-derived lineages in hard tissue was < 20% per total cells at 1-year-old. Similarly, LepR+ PDL cells differentiated into osteocytes following tooth extraction, but their frequency was < 9%. Additionally, both LepR+ and LepR− PDL cells demonstrated spheroid-forming capacity, which is an indicator of self-renewal. These results indicate that both LepR+ and LepR− PDL populations contributed to hard tissue formation. LepR− PDL cells increased the expression of LepR during spheroid formation, suggesting that the LepR− PDL cells may hierarchically sit upstream of LepR+ PDL cells. Collectively, the origin of hard tissue-forming cells in the PDL is heterogeneous, some of which express LepR.
Detecting red-lesions from retinal fundus images using unique morphological features
Authors: Monemian Maryam , Rabbani Hossein 
Published: March 2023

AbstractOne of the most important retinal diseases is Diabetic Retinopathy (DR) which can lead to serious damage to vision if remains untreated. Red-lesions are from important demonstrations of DR helping its identification in early stages. The detection and verification of them is helpful in the evaluation of disease severity and progression. In this paper, a novel image processing method is proposed for extracting red-lesions from fundus images. The method works based on finding and extracting the unique morphological features of red-lesions. After quality improvement of images, a pixel-based verification is performed in the proposed method to find the ones which provide a significant intensity change in a curve-like neighborhood. In order to do so, a curve is considered around each pixel and the intensity changes around the curve boundary are considered. The pixels for which it is possible to find such curves in at least two directions are considered as parts of red-lesions. The simplicity of computations, the high accuracy of results, and no need to post-processing operations are the important characteristics of the proposed method endorsing its good performance.
Sleep duration, hypnotic drug use, and risk factors: cross- sectional study
Authors: Jalali Nazanin , Khalili Parvin , Jamali Zahra , Jalali Zahra , Moghadam-Ahmadi Amir , Vakilian Alireza , Ayoobi Fatemeh 
Published: March 2023

AbstractBoth short sleep duration (SSD) and long sleep duration (LSD) are associated with an increased risk of morbidity and mortality. Here, we aimed to assess the prevalence of sleep duration disturbances among adults in association with demographic, medication use, personal habits, and chronic diseases, while also considering the impact of hypnotic drug use. We performed a cross-sectional study of 9991 adult participants of the Rafsanjan Cohort Study (RCS), as part of the Prospective epidemiological research studies in Iran (PERSIAN). Multivariate logistic regression analyses were conducted to assess the association between short (< 6 h) and long (> 9 h) sleep duration with demographic and lifestyle parameters and common non-communicable diseases. Additionally, we performed stratified analysis to investigate the association of sleep duration with the abovementioned factors and diseases, in groups with and without hypnotic drug use. We found higher odds of SSD significantly associated with age (P < 0.001), BMI (P < 0.001), physical activity (P < 0.001), and depression (P = 0.023). LSD displayed a positive association with the female sex (P < 0.001), opium consumption (P < 0.001), and history of MI (P = 0.045), and a reverse connection with education (P = 0.007), physical activity (P < 0.001) and alcohol consumption (P = 0.027). Stratifying for the hypnotic drug use, our sensitivity analyses indicated that in hypnotic drug users, education (P = 0.034) and physical activity (P < 0.001) were associated with LSD, in this group, significantly increased odds ratio of LSD were associated with opium consumption (P = 0.046) and thyroid dysfunction (P = 0.037). Our findings demonstrated the demographic and lifestyle factors and diseases associated with long and short sleep duration in the population of the RCS. Additionally, after stratifying for hypnotic drug use, our results indicated that some diseases are only associated with abnormal sleep duration upon using hypnotic drugs.
The interactions of monomeric acridines and unsymmetrical bisacridines (UAs) with DNA duplexes: an insight provided by NMR and MD studies
Authors: Laskowski Tomasz , Kosno Michał , Andrałojć Witold , Frackowiak Joanna E. , Borzyszkowska-Bukowska Julia , Szczeblewski Paweł , Radoń Nikola , Świerżewska Maria , Woźny Anna , Paluszkiewicz Ewa , Mazerska Zofia 
Published: March 2023

AbstractMembers of a novel class of anticancer compounds, exhibiting high antitumor activity, i.e. the unsymmetrical bisacridines (UAs), consist of two heteroaromatic ring systems. One of the ring systems is an imidazoacridinone moiety, with the skeleton identical to the structural base of Symadex. The second one is a 1-nitroacridine moiety, hence it may be regarded as Nitracrine’s structural basis. These monoacridine units are connected by an aminoalkyl linker, which vary in structure. In theory, these unsymmetrical dimers should act as double-stranded DNA (dsDNA) bis-intercalators, since the monomeric units constituting the UAs were previously reported to exhibit an intercalating mode of binding into dsDNA. On the contrary, our earlier, preliminary studies have suggested that specific and/or structurally well-defined binding of UAs into DNA duplexes might not be the case. In this contribution, we have revisited and carefully examined the dsDNA-binding properties of monoacridines C-1305, C-1311 (Symadex), C-283 (Ledakrin/Nitracrine) and C-1748, as well as bisacridines C-2028, C-2041, C-2045 and C-2053 using advanced NMR techniques, aided by molecular modelling calculations and the analysis of UV–VIS spectra, decomposed by chemometric techniques. These studies allowed us to explain, why the properties of UAs are not a simple sum of the features exhibited by the acridine monomers.
Relationship between iliopsoas muscle surface pressure and implant alignment after total hip arthroplasty: a cadaveric study
Authors: Tamaki Yasuaki , Goto Tomohiro , Iwase Joji , Wada Keizo , Omichi Yasuyuki , Hamada Daisuke , Tsuruo Yoshihiro , Sairyo Koichi 
Published: March 2023

AbstractIliopsoas impingement after total hip arthroplasty is caused by the implant irritating the iliopsoas muscle, but changes in the iliopsoas muscle have not been quantitatively evaluated. This study assessed changes in the surface pressure of the iliopsoas muscle when the implant alignment was varied. Total hip arthroplasty was performed in 10 fresh-frozen cadaveric hips. We evaluated the maximum and mean surface pressure of the iliopsoas muscle with the hip in 20° and 10° extension, the neutral position, and 10° flexion when the anterior cup protrusion length (ACPL), stem version, and stem offset were varied. When the ACPL was changed to 0, 3, and 6 mm in 20° extension, the maximum surface pressure was significantly increased for ACPL of 6 mm compared with 0 mm. Decreased stem anteversion resulted in a significant reduction in both the maximum and mean surface pressure compared with native anteversion from 20° extension to the neutral position. Increased stem offset resulted in significant increases in the maximum and mean surface pressure of the iliopsoas muscle compared with decreased stem offset in 20° extension. Not only large ACPL but also changes in stem version and offset affected the maximum surface pressure of the iliopsoas muscle.
Interferon regulatory factor 1 (IRF1) inhibits lung endothelial regeneration following inflammation-induced acute lung injury
Authors: Chen Xiaorui , Qi Di , Fan Shulei , He Yirui , Jing Hekun , Wang Daoxin 
Published: March 2023

Abstract
Background: Acute respiratory distress syndrome (ARDS) is a respiratory condition caused by severe endothelial barrier dysfunction within the lung. In ARDS, excessive inflammation, tissue edema, and immune cell influx prevents endothelial cell regeneration that is crucial in repairing the endothelial barrier. However, little is known about the molecular mechanism that underpin endothelial cell regeneration in ARDS.
Methods: R-based bioinformatics tools were used to analyze microarray-derived transcription profiles in human lung microvascular endothelial cells (HLMVECs) subjected to non-treatment or lipopolysaccharide (LPS) exposure. We generated endothelial cell-specific interferon regulatory factor 1 (Irf1) knockout (Irf1EC-/−) and Irf1fl/fl control mice for use in an endotoxemic murine model of acute lung injury (ALI). In vitro studies (qPCR, immunoblotting, and ChIP-qPCR) were conducted in mouse lung endothelial cells (MLECs) and HLMVECs. Dual-luciferase promoter reporter assays were performed in HLMVECs.
Results: Bioinformatics analyses identified IRF1 as a key up-regulated gene in HLMVECs post-LPS exposure. Endothelial-specific knockout of Irf1 in ALI mice resulted in enhanced regeneration of lung endothelium, while liposomal delivery of endothelial-specific Irf1 to wild-type ALI mice inhibited lung endothelial regeneration in a leukemia inhibitory factor (Lif)-dependent manner. Mechanistically, we demonstrated that LPS-induced Stat1Ser727 phosphorylation promotes Irf1 transactivation, resulting in downstream up-regulation of Lif that inhibits endothelial cell proliferation.
Conclusions: These results demonstrate the existence of a p-Stat1Ser727-Irf1-Lif axis that inhibits lung endothelial cell regeneration post-LPS injury. Thus, direct inhibition of IRF1 or LIF may be a promising strategy for enhancing endothelial cell regeneration and improving clinical outcomes in ARDS patients.
Fast geolocation solution for bistatic interferometric synthetic aperture radar configuration of inclined geosynchronous transmitter with low earth orbit receivers
Authors: Dai Chao , Tian Feng , Suo Zhiyong 
Published: March 2023

AbstractSpaceborne interferometric synthetic aperture radar (InSAR) technology is an effective method to obtain digital elevation model (DEM) data. The bistatic InSAR configuration of the inclined‐geosynchronous (InGEO) transmitter with low earth orbit (LEO) receivers (InGEO‐LEO) is a novel InSAR system to acquire terrain information. This novel system is characterised with high resolution, wide swath and timeliness, but the Newton iterative method is time‐consuming to solve the bistatic InSAR equations for fast DEM generation. For the conventional LEO bistatic InSAR system, the closed‐form solution is an effective method to improve the efficiency of solving InSAR equations, which is invalid in the InGEO‐LEO InSAR system because of the significant geometry difference caused by the orbits of InGEO transmitter and LEO receivers. To address this issue, we analyse the bistatic InGEO‐LEO geometry in detail and exploit the bistatic InSAR equations to propose an approximate closed‐form solution (ACS) for the novel system. Compared with the general Newton iterative method, the ACS significantly improves the efficiency of geolocation for the bistatic InGEO‐LEO InSAR system with high precision. Simulation experiments are carried out to verify the effectiveness and superiority of ACS.
Migration of pairs of giant planets in low-viscosity discs
Authors: Griveaud P. , Crida A. , Lega E. 
Published: March 2023

Context. When considering the migration of Jupiter and Saturn, a classical result is to find the planets migrating outwards and locked in the 3:2 mean motion resonance (MMR). These results were obtained in the framework of viscously accreting discs, in which the observed stellar accretion rates constrained the viscosity values. However, it has recently been shown observationally and theoretically that discs are probably less viscous than previously thought.
Aims. Therefore, in this paper, we explore the dynamics of pairs of giant planets in low-viscosity discs.
Methods. We performed two-dimensional hydrodynamical simulations using the grid-based code FARGOCA.
Results. In contrast to classical viscous discs, we find that the outer planet never crosses the 2:1 resonance and the pair does not migrate outwards. After a wide parameter exploration, including the mass of the outer planet, we find that the planets are primarily locked in the 2:1 MMR and in some cases in the 5:2 MMR. We explain semi-analytically why it is not possible for the outer planet to cross the 2:1 MMR in a low-viscosity disc.
Conclusions. We find that pairs of giant planets migrate inwards in low-viscosity discs. Although, in some cases, having a pair of giant planets can slow down the migration speed with respect to a single planet. Such pairs of slowly migrating planets may be located, at the end of the disc phase, in the population of exoplanets of ’warm Jupiters’. However, the planets never migrate outwards. These results could have strong implications on the Solar System’s formation scenarios if the Sun’s protoplanetary disc had a low viscosity.
Assessing critical point signatures through proton intermittency in NA61/SHINE
Authors: Davis Nikolaos 
Published: March 2023

The search for experimental signatures of the critical point (CP) of strongly interacting matter is one of the main objectives of the NA61/SHINE experiment at CERN SPS. One such candidate observable is local fluctuations of the proton density in transverse space, constituting an order parameter of the chiral phase transition, and expected to scale according to a universal powerlaw in the vicinity of the CP. Its scaling can be probed through an intermittency analysis of the proton second scaled factorial moments (SSFMs) in transverse momentum space. The first such analysis [1] revealed power-law behavior in NA49 Si+Si collisions at 158A GeV/c, with a fitted exponent value consistent with the critical prediction, within errors.
In the present work, we discuss known challenges posed by standard intermittency analysis in the face of low to moderate event statistics, and propose a novel technique that solves them. We perform a scan of Monte Carlo models simulating alternative critical and background scenarios, and weigh them against experimental results; we explore the method’s efficacy on simulated data. Once adapted to the available NA61/SHINE systems, the method will allow us to obtain reliable confidence intervals for the intermittency index (power-law ex-ponent) ϕ2 compatible with the experimental data.
Event-shape studies of strangeness production in √S = 13 TeV proton–proton collisions with ALICE
Authors: Nassirpour Adrian 
Published: March 2023

The ALICE Collaboration has observed that the ratio of strange to non-strange hadron yields increases strongly with multiplicity in small collision systems at LHC energies. The origin of this effect is still not fully understood. Models need to incorporate final-state interactions to accommodate the new ALICE results, and require new measurements to discriminate between the various phenomenological descriptions.
Two different ALICE studies, aiming to further elucidate the underlying mechanisms of strangeness production in high-multiplicity proton–proton (pp) collisions at √S = 13 TeV, are presented in this proceeding. First, the production of η and π0 are studied as a function of multiplicity, combining several analysis techniques, allowing a measurement for the neutral meson production over a vast transverse momentum (pT) range. This is followed by a measurement of π, K, ϕ, and Ξ as a function of the unweighed transverse spherocity SOPT=1, an event-shape observable which allows us to explore particle production in azimuthal topologies dominated by either hard or soft QCD processes. The results are compared to predictions from PYTHIA and EPOS-LHC.
Früherkennung und Prävention des Typ-1-Diabetes
Authors: Hofelich Anna , Marcus Benjamin A. , Achenbach Peter 
Published: March 2023

ZUSAMMENFASSUNGTechnologischer Fortschritt und das Verständnis der zugrunde liegenden Immunpathogenese der Erkrankung Diabetes mellitus Typ 1 (T1D) haben zu Veränderungen in den präventiven und therapeutischen Ansätzen geführt. Diese zielen darauf ab, das Entstehen des Autoimmunprozesses zu verhindern oder dessen Voranschreiten zumindest zu verlangsamen bzw. die Zerstörung von Betazellen aufzuhalten oder hinauszuzögern. Die Zulassung erster Medikamente für den Einsatz bei Kindern und Jugendlichen im fortgeschrittenen Frühstadium oder kurz nach klinischer Manifestation ist bereits in Aussicht 1.