bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial neural networks optimize the establishment of a Brazilian germplasm core collection of winter squash (Cucurbita moschata D.)
Authors: Gomes Ronaldo Silva , Júnior Ronaldo Machado , de Almeida Cleverson Freitas , de Oliveira Rebeca Lourenço , Nascimento Moysés , Nardino Maicon , Silva Derly José Henriques da 
Published: April 2023

Abstract

With widespread cultivation, Cucurbita moschata stands out for the carotenoid content of its fruits such as β and α-carotene, components with pronounced provitamin A function and antioxidant activity. C. moschata seed oil has a high monounsaturated fatty acid contente and vitamin E, constituting a lipid source of high chemical-nutritional quality. The present study evaluate the agronomic and chemical-nutritional aspects of 91 accessions of C. moschata kept at the BGH-UFV and propose the establishment of a core collection based on multivariate approaches and on the implementation of Artificial Neural Networks (ANNs). ANNs was more efficient in identifying similarity patterns and in organizing the distance between the genotypes in the groups. The averages and variances of traits in the CC formed using a 15% sampling of accessions, were closer to those of the complete collection, particularly for accumulated degree days for flowering, the mass of seeds per fruit, and seed and oil productivity. Establishing the 15% CC, based on the broad characterization of this germplasm, will be crucial to optimize the evaluation and use of promising accessions from this collection in C. moschata breeding programs, especially for traits of high chemical-nutritional importance such as the carotenoid content and the fatty acid profile.
CHMP4C Deficiency Represses Angiogenesis in Chronic Ischemia by Inhibiting Hypoxia-induced Wnt/β-catenin Signaling Activation via GSK3β
Authors: Liu Wen , Ding Suling , Zhang Zhiwei , Zhou Nianwei , Li Xuejie , Xie Huilin , Tian Fangyan , Weng Haobo , Liu Ao , Bao Pei , Zhu Xiaowei , Yang Xiangdong , Shu Xianhong 
Published: April 2023

Abstract
Aims
Angiogenesis is critical for tissue repair in chronic ischemia. Chromatin modified protein 4C (CHMP4C) is a subunit of the endosomal sorting complex required for transport -III(ESCRT-III), which is involved in endocytic progress and cell proliferation. Recent evidence suggests ESCRT-III plays a vital role in endothelial functioning. This study aimed to determine the role of endothelial CHMP4C in angiogenesis, as well as the underlying molecular mechanisms.
Methods and Results
Hind-limb ischemia was surgically induced in both CHMP4C-/- mice and wild-type C57BL/6J mice. Loss of CHMP4C was associated with significant decreases in blood perfusion and capillary density post-ischemia. In vitro, knockdown of CHMP4C by small interfering RNAs(siRNA) impaired the angiogenic and proliferate functions of ECs and induces G1/S cell cycle arrest without apoptotic effects under hypoxic conditions. RNA-Seq data and further analysis revealed the repression of Wnt/β-catenin pathway and hyperactivation of GSK3β in CHMP4C-deficiency ECs. And selective inhibition of GSK3β significantly ameliorated the inhibitory effects of CHMP4C deficiency on the Wnt/β-catenin pathway and proliferate functions in vitro. Electron microscopy and immunohistochemical colocalization analysis revealed that the CHMP4C deficiency impedes endocytic trafficking of GSK3β. Additionally, co-immunoprecipitation and proteomic analysis demonstrated CHMP4C interacted with PP2Ac and regulate GSK3β activity. The interaction between CHMP4C and PP2Ac may be crucial for GSK3β endocytic trafficking.
Conclusion
CHMP4C deficiency suppresses angiogenesis in chronic ischemia by disrupting Wnt/β-catenin signaling via GSK3β. The mechanism is associated with suspension of GSK3β endocytic trafficking.
TcrXY is an acid-sensing two-component transcriptional regulator of Mycobacterium tuberculosis and a novel target for enhanced TB therapy
Authors: Stupar Miljan , Tan Lendl , Kerr Edward , De Voss Christopher , Forde Brian , Schulz Benjamin , West Nicholas P. 
Published: April 2023

Abstract

The ability of Mycobacterium tuberculosis (Mtb) to persist in the host complicates and prolongs tuberculosis (TB) patient chemotherapy. Here we demonstrate that a neglected two-component system (TCS) of Mtb, TcrXY, is an autoregulated acid-sensing TCS that controls a functionally diverse 117-gene regulon required for bacterial persistence. Characterisation of two representatives of this regulon, Rv3706c and Rv3705A, implicate these genes as key determinants for the survival of Mtb in vivo by serving as important effectors to mitigate redox stress at acidic pH. We show that genetic silencing of the response regulator tcrX using CRISPR interference attenuates the persistence of Mtb during chronic mouse infection and improves treatment with the two front-line anti-TB drugs, rifampicin and isoniazid. We propose that targeting TcrXY signal transduction blocks the ability of Mtb to sense and respond to acid stress, resulting in a disordered program of persistence to render the organism vulnerable to existing TB chemotherapy.
Nitrogen dynamics as a function of soil types, compaction, and moisture
Authors: Das Saurav , Mohapatra Ankita , Sahu Karubakee , Panday Dinesh , Ghimire Deepak , Maharjan Bijesh 
Published: April 2023

Abstract
The complex interactions between soil types, compaction, and moisture on nitrogen transformation processes, such as ammonia volatilization, ammonification, nitrification, and denitrification, were examined using a simulated columns study. Over the course of 30 days, two different soil types—loam and sandy loam—were subjected to three different compaction treatments (control, surface, and sub-surface compaction), and two different moisture treatments (dry and wet). Urea ammonium nitrate (32-0-0) was used as nitrogen fertilizer at the rate of 200 kg N/ha. Measurements were made of important indicators of nitrogen transformations, such as residual concentrations of ammonium and nitrate, nitrate leaching, ammonia volatilization, and nitrous oxide emissions. The findings showed that compaction increased residual ammonium concentrations in deeper soil profiles and decreased nitrification rates in both soil types. Loam soil showed increased nitrate leaching due to higher rates of nitrification and residual nitrate concentrations. The interaction of compaction and moisture had the greatest impact on nitrous oxide emissions, with the highest emissions observed in control treatments during simulated dry weather. The results highlight the significance of taking soil texture, moisture, and compaction into account when implementing sustainable nitrogen management techniques in agriculture. Based on these findings, a number of recommendations are put forth for effective nitrogen management, including the avoidance of broadcast application in moist soil to reduce ammonia volatilization and improve nitrogen use efficiency, and split application to reduce potential nitrate leaching and nitrous oxide emissions from loam soil.
Improving Detection of ChatGPT-Generated Fake Science Using Real Publication Text: Introducing xFakeBibs a Supervised-Learning Network Algorithm
Authors: Hamed Ahmed Abdeen , Wu Xindong 
Published: April 2023

Abstract
ChatGPT is becoming a new reality. In this paper, we show how to distinguish ChatGPT-generated publications from counterparts produced by scientists. Using a newly designed supervised Machine Learning algorithm, we demonstrate how to detect machine-generated publications from ones produced by scientists. The algorithm was trained using 100 real publications, calibrated by 10-fold of real publications. When comparing the training with calibration, we found that the similarities fluctuated between (19%-21%) of bigram overlaps. The calibrating folds contributed (51%-70%) of new bigrams, while ChatGPT contributed only 23% (> 50% of any of the other 10 calibrating folds). When classifying the individual articles, the xFakeBibs algorithm predicted 98/100 publications as fake, while 2 articles failed the test and were classified as real publications. We introduced an algorithmic approach that detected the ChatGPT-generated articles with a high degree of accuracy. However, it remains challenging to detect all fake records. This work is indeed a step in the right direction to counter fake science and misinformation.
Analysis of Link between Brain Insulin Resistance and Alzheimer's Disease: A Systematic Review
Authors: Andrade Luís Jesuino de Oliveira , de Oliveira Luís Matos , Bittencourt Alcina Maria Vinhaes , Lourenço Letícia Góes de Carvalho , de Oliveira Gabriela Correia Matos 
Published: April 2023

Abstract
Introduction
Insulin resistance (IR) is a condition characterized by the inability of cells to respond to insulin, leading to glucose intolerance and hyperglycemia. This condition has been well-documented in peripheral tissues such as liver, muscle, and adipose tissue. However, recent studies suggest that the IR in the brain, also known as brain IR, may play a role in the development of neurodegenerative diseases such as Alzheimer's disease (AD).
Objective
In this systematic review, we aim to summarize the current knowledge about brain insulin resistance (BIR) and its potential implications in neurodegenerative diseases.
Methods
A systematic search of PubMed, Medline, EMBASE, and the Cochrane Central Register of Controlled Trials was conducted using the keywords "insulin resistance", “brain insulin resistance”, "Alzheimer's disease", "neurodegeneration", and "cognition". The search was limited to articles published in English from 2000 to 2023. Studies were included if they investigated the role of BIR in neurodegenerative diseases or if they provided insights into the mechanisms involved in BIR. A total of 17 articles were included in this review.
Results
BIR has been observed in various neurodegenerative diseases such as AD. Studies suggest that BIR may contribute to the cognitive impairment seen in these diseases. Insulin plays an important role in regulating glucose metabolism, neuronal energy production, and synaptic plasticity in the brain. BIR leads to impaired glucose uptake and utilization, decreased ATP production, and alterations in synaptic plasticity, which ultimately affect cognitive function.
Conclusion
BIR is emerging as an important factor in the pathogenesis of neurodegenerative diseases, particularly AD. By targeting BIR, novel therapeutic strategies may be developed for the treatment and prevention of these devastating diseases.
NSUN5 facilitates the progression of hepatocellular carcinoma by increasing the expression of SMAD3
Authors: Han Hexu , Du Yanping , Zhao Zhibin , Wang Yifan , Zhang Jing , You Xiaomin , Zhou Guoxiong , Zhou Huilin , Ye Jun , Tian Weizhong , Yu Hong 
Published: April 2023

Abstract
Hepatocellular carcinoma (HCC), the most prevalent liver cancer, makes patients' prognosis extremely poor due to frequent intrahepatic and distant metastasis. The epithelial-mesenchymal transition (EMT) plays an important role in this process. However, the content of NSUN5 in hepatocellular carcinoma and whether NSUN5 is involved in mediating the epithelial-mesenchymal transition of hepatocellular carcinoma remains unknown. In this study, based on clinicopathologic analyses of several independent HCC cohorts and the results of induced tumor formation in Nsun5 knockout mice, we observed that NSUN5 expression was increased in tumor tissues. The absence of Nsun5 would retard the malignant progression of hepatocellular carcinoma, suggesting that Nsun5 might be an important oncogene in HCC. Furthermore, we found that high expression of NSUN5 promotes EMT in HCC cells. After NSUN5 is knocked out, the HCC cell's ability to invade and migrate decreases in vivo and vitro conditions; on the other hand, NSUN5 overexpression in HCC cells had the opposite effect. Mechanically, the highly expressed NSUN5 in cancer tissues promotes the enrichment of Tri-Methyl-histone H3 (Lys4) in the promoter region of SMAD3 by interacting with WDR5, thereby promoting hepatocellular carcinoma metastasis by SMADd3-mediated EMT. In general, we identified NSUN5 as a novel promoter of metastasis in hepatocellular carcinoma and established an innovative theoretical foundation for treating this disease.
Effective first flush volumes in experimental household-scale rainwater catchment systems
Authors: Charlebois Bridgette , Wittbold Patrick , Reckhow David , Kumpel Emily 
Published: April 2023

Abstract

Rainwater collection systems often include a first flush system to divert contaminants away from collected and stored rainwater. These have traditionally been designed for a set volume, to capture and divert the first 1–2 mL of rain deposited onto a roof. However, environmental and collection system parameters can vary the volume of the first flush necessary to effectively divert contaminants. Using a test-scale rainwater collection system in Amherst, Massachusetts (USA), a series of experiments were conducted to assess the quality of rainwater in the system per collection volume and time. This included a tracer study of an ideal contaminant, and water quality measurements of atmospheric rain, fractionated first flush, and the collection tank during rain events. First flush samples contained elevated dissolved organic carbon (DOC) concentrations up to 40 mg/L, with high variability between the rain events. UV 254, DOC, and conductivity all trended together indicating a uniform wash-off of contaminants. Higher intensity storms increased roof wash-off deposition and environmental conditions affected the necessary first flush volumes. The majority of contaminants likely originated from roof wet and dry deposition. The design of first flush in rainwater harvesting systems needs to account for local precipitation patterns, storm intensity, and canopy conditions.
Role of cationic surfactants in palladium adsorption of commercial ion exchange resins using electroless plating solutions
Authors: Rajesh Yennam , Jeeru Lakshmana Rao , Dabhade Ganesh , Daware Gaurav 
Published: April 2023

AbstractThis work investigates the role of cationic surfactants in the adsorption of palladium ions from synthetic electroless plating solutions using a commercial resin, Lewatit TP-214. This would also help us in determining the batch adsorption experiments elaborated on the optimal parameters such as surfactant concentration, pH, dosage, initial metal ion concentration for the development of an ion-exchange resin with high metal removal efficiency. Critical micelle concentration (CMC) appears to be an important parameter in determining the adsorption behavior of ion-exchange resins with palladium ions. Equilibrium models were measured for their fitness with the obtained Pd (II) batch adsorption characteristics and Freundlich isotherm confirms the heterogeneous Pd (II) adsorption on Lewatit TP-214. FTIR analysis confirmed that the Pd (II) metal uptake of Lewatit TP-214 resin largely depends on amine groups (-NH2+ and -NH+) and the donor atoms attached to cationic surfactant. The optimized choice of adsorption parameters (pH of 8, dosage of 1 g/L, and contact time of 300 min) of Lewatit TP-214 adsorbent provided the highest metal uptake and removal efficiency as 201.7 mg/g and 90.16%, respectively, for the lowest Pd concentration of 300 mg/L.
Use and Acceptance of Smart Elderly Care Apps Among Chinese Medical Staff and Older Individuals: Web-Based Hybrid Survey Study
Authors: Zhu Jieting , Weng Huiting , Ou Peng , Li Lezhi 
Published: April 2023

Background
With the advent of China’s aging population and the popularization of smartphones, there is a huge demand for smart elderly care apps. Along with older adults and their dependents, medical staff also need to use a health management platform to manage the health of patients. However, the development of health apps and the large and growing app market pose a problem of declining quality; in fact, important differences can be observed between apps, and patients currently do not have adequate information and formal evidence to discriminate among them.

Objective
The aim of this study was to investigate the cognition and usage status of smart elderly care apps among older individuals and medical staff in China.

Methods
From March 1, 2022, to March 30, 2022, we used the web survey tool Sojump to conduct snowball sampling through WeChat. The survey links were initially sent to communities in 23 representative major cities in China. We asked the medical staff of community clinics to post the survey link on their WeChat Moments. From April 1 to May 10, 2022, we contacted those who selected “Have used a smart elderly care app” in the questionnaire through WeChat for a request to participate in semistructured interviews. Participants provided informed consent in advance and interviews were scheduled. After the interviews, the audio recordings were transcribed into text and the emerging themes were analyzed and summarized.

Results
A total of 810 individuals participated in this study, 54.8% (n=444) of whom were medical staff, 33.1% (n=268) were older people, and the remaining participants were certified nursing assistants (CNAs) and community workers. Overall, 60.5% (490/810) of the participants had used a smart elderly care app on their smartphone. Among the 444 medical staff who participated in the study, the vast majority (n=313, 70.5%) had never used a smart elderly care app, although 34.7% of them recommended elderly care–related apps to patients. Among the 542 medical staff, CNAs, and community workers that completed the questionnaire, only 68 (12.6%) had used a smart elderly care app. We further interviewed 23 people about their feelings and opinions about smart elderly care apps. Three themes emerged with eight subthemes, including functional design, operation interface, and data security.

Conclusions
In this survey, there was a huge difference in the usage rate and demand for smart elderly care apps by the participants. Respondents are mainly concerned with app function settings, interface simplicity, and data security.
Analisis Kepuasan Tenaga Kerja Wanita Terhadap Kebijakan Jaminan Ketenagakerjaan di SPA at Maya Sanur
Authors: Antari Desak Putu Putri Maya , Susanti Luh Eka 
Published: April 2023

Tenaga kerja merupakan pelakon pembangunan serta pelakon ekonomi baik secara orang ataupun secara kelompok, sehingga memiliki peranan yang sangat signifikan dalam kegiatan perekonomian nasional, ialah tingkatkan produktivitas serta kesejahteraan warga. Pada masa saat ini ini, perempuan turut berpartisipasi tingkatkan kesejahteraan keluarga dengan metode bekerja ialah perihal biasa. Riset ini bertujuan buat mengenali anggapan serta tingkatan Kepuasan Tenaga Kerja Perempuan Terhadap Kebijakan Jaminan Ketenagakerjaan di Spa at Maya Sanur. Perlengkapan ukur yang digunakan buat mengenali kebijakan jaminan ketenagakerjaan yang diperoleh tenaga kerja perempuan di Spa at Maya Sanur merupakan Undang- Undang Nomor 13 Tahun 2003 tentang ketenagakerjaan. Metode analisis informasi yang digunakan dalam riset ini merupakan deskriptif kualitatif. Metode pengumpulan informasi dicoba dengan metode observasi serta wawancara. Berikutnya informasi yang didapat hendak dibanding dengan hak serta proteksi cocok Undang- Undang tentang ketenagakerjaan. Dari hasil riset yang didapat terdapat sebagian kebijakan jaminan yang telah diperoleh ialah cuti pada masa kehamilan, hak buat menyusui, proteksi jam malam. Ada pula jaminan tidak diperoleh oleh tenaga kerja perempuan di Spa at Maya Sanur antara lain bayaran persalinan serta cuti haid. Ada pula anjuran yang diberikan kepada Spa at Maya Sanur supaya lebih tingkatkan kebijakan jaminan yang belum diberikan kepada tenaga kerja perempuan.

Workers are development actors and economic actors both individually and in groups, so they have a very significant role in national economic activities, namely increasing productivity and community welfare. At this time, women participate in improving the welfare of the family by working is common. This study aims to determine the perception and level of satisfaction of female workers on the Employment Guarantee Policy at Spa at Maya Sanur. The measuring instrument used to determine the employment security policy obtained by female workers at Spa at Maya Sanur is Law No. 13 of 2003 concerning employment. The data analysis technique used in this research is descriptive qualitative. Data collection techniques were carried out by means of observation and interviews. Furthermore, the data obtained will be compared with the rights and protections according to the Law on Manpower. From the results of the research, there are several insurance policies that have been obtained, namely leave during pregnancy, the right to breastfeed, curfew protection. The guarantees not obtained by female workers at Spa at Maya Sanur include delivery costs and menstrual leave. The advice given to Spa at Maya Sanur is to further improve the insurance policy that has not been given to female workers.
Penerapan Konsep Tri Hita Karana pada Aspek Palemahan dalam Pengelolaan Limbah Sampah di The Wakanda Ubud
Authors: Yuliani Dewa Ayu Dwi Deviari , Susanti Luh Eka 
Published: April 2023

Tri Hita Karana merupakan tuntunan keharmonisan di Bali. Namun banyak akomodasi di Bali yang belum mengimplementasikan konsep Tri Hita Karana terutama pada aspek palemahan khususnya kelestarian lingkungan dan pengelolaan limbah sampah yang belum dilakukan secara optimal salah satunya yaitu The Wakanda Ubud. Penelitian ini bertujuan untuk mengetahui bagaimana penerapan aspek palemahan terhadap pengelolaan limbah sampah di The Wakanda Ubud. Data primer dan data sekunder adalah jenis sumber data yang digunakan pada penelitian ini dimana data didapat dari sumbernya langsung dan sumber lain-lain. Dilengkapi dengan teknik pengumpulan data observasi dan wawancara dimana mengamati secara langsung dan mendapatkan keterangan dari narasumber yang bersangkutan dalam penelitian ini. Menurut hasil peelitian, The Wakanda Ubud sudah menerapkan aspek palemahan terhadap pengelolaan sampah dengan baik namun tidak maksimal. Salah satunya yaitu sampah organik yang diangkut begitu saja tanpa diolah. Adapun saran yang diberikan adalah pihak The Wakanda Ubud setidaknya bekerja sama dengan pihak Dinas Kebersihan Gianyar untuk mengolah limbah organi kagar dapat lebih bermanfaat bagi hotel dan lingkungan.

Many hotels in Bali implemented the Tri Hita Karana as a guide of harmony. However, many hotels in Bali have not implemented the Tri Hita Karana concept, especially in the palemahan aspect of the environmental sustainability and waste management that has not been carried out optimally, one of them is The Wakanda Ubud. This study is aims find out how the palemahan aspect is applied to waste management. This study is use primary and secondary data sources. Completed with data collection techniques by observation and interviews wile observing directly and getting information from the relevant sources. According to research results, The Wakanda Ubud has implemented the palemahan aspect of waste management properly but not optimally. One of them is organic waste that is transported without being processed. The advice given is that The Wakanda Ubud at least cooperates with the Gianyar Cleaning Service to process organic waste so that it can be more beneficial for the hotel and the environment.