bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesure de la digestibilité <em>in vivo</em> de résidus de récolte de quatre variétés améliorées de sorgho à double usage sur les moutons de race Djallonké
Authors: Ouédraogo Souleymane , Sanou Lassina , Gnegne Issaka , Sere Modou , Adam Myriam , Savadogo Patrice , Kaboré Zoungrana Chantal Yvette 
Published: January 2025

Contexte : Avec la réduction de s surfaces de parcours naturels et de leur productivité, l’alimentation des animaux doit se tourner vers d’autres ressources, notamment les coproduits de récolte. Objectif : Cette étude avait pour objectif de comparer la digestibilité in vivo de variétés de sorgho améliorées afin d’identifier celles qui pourraient répondent mieux au double usage : production de grains et utilisation des pailles comme fourrage. Méthodes : Des essais ont été conduits sur quatre variétés de sorgho : Fadda, Grinkan, Djiguikala et Zalatimi. L’ingestion et la digestibilité des pailles ont été évaluées sur 12 béliers adultes de race Djallonké, dans des cages de digestibilité avec collecte totale des fèces. Le régime était constitué de paille de sorgho broyée servie ad libitum, avec une complémentation de tourteau de coton. Les rations offertes et refusées ont été analysées et ont permis la détermination de la digestibilité apparente de la matière sèche (dMS), de la matière organique (dMO) et des matières azotées totales (dMAT). Résultats : Les pailles de sorgho avaient des teneurs en matières azotées totales (MAT) entre 2,9 et 5,7 % de la matière sèche (MS). L’ingestion a été la plus élevée pour la variété Grinkan (366,9 g/j) et la plus faible pour la variété Djiguikala (266,4 g/j). Les plus fortes valeurs de dMS, dMO et dMAT ont été obtenues avec la variété Zalatimi avec 44,4 %, 43,5 % et 42,4 % respectivement. La plus faible dMS a été observée avec la variété Djiguikala (37,3 %) et les plus faibles dMO et dMAT avec la variété Fadda (35,8 % et 24,7 % respectivement). Conclusions : Les variétés avec les meilleures digestibilités, Grinkan et Zalatimi, sont aussi celles qui ont une meilleure ingestibilité, et elles ont des teneurs en MAT convenables. Ces premiers résultats montrent que certaines variétés de sorgho sont a priori plus favorables pour l’alimentation animale, mais ils doivent être complétés par des essais zootechniques sur une plus longue période.
Resection of Heterotopic Ossifications and Total Hip Arthroplasty Using a Single Ludloff Medial Approach
Authors: Simon Dominic , Lerchenberger Maximilian , Arnholdt Jörg , Holzapfel Boris M. , Beckers Gautier 
Published: January 2025

Case:
Heterotopic ossification (HO) is a relatively rare but severe clinical finding around the hip joint, characterized by the formation of extraskeletal bone in soft tissue. We present the case of a 66-year-old man with a severe, painful gait disorder caused by extensive neurogenic bilateral HO. In this case, due to the medial HO localization, we performed a staged bilateral, combined HO resection and total hip arthroplasty using the single medial Ludloff approach.

Conclusion:
Our case highlights that, for combined hip osteoarthritis and medial HO, a Ludloff approach can be an effective option for achieving favorable surgical outcomes.
Experimental Research on High-Voltage Electric Pulse Rock-Breaking Performance
Authors: Liu Weiji , Tang Wuji , Zhu Xiaohua , Zhou Xin , Zhang Youjian 
Published: January 2025

Summary
High-voltage electric pulse (HVEP) drilling technology offers advantages such as high rock-breaking efficiency and low energy consumption. However, its effectiveness is influenced by parameters including pulse voltage magnitude and the shape and structure of the high-voltage electrode drill bit (HVED). Currently, there is limited research on the mechanisms by which high pulse voltages (&gt;100 kV) affect rock dielectric breakdown and the patterns of pulse voltage generation. To better reflect the impact of various parameters on rock-breaking performance during HVEP drilling, this study conducts laboratory experiments on HVEP rock breaking. The effects of pulse voltage magnitude, different rock samples, and various HVED shapes on HVEP rock-breaking efficiency were investigated. The experimental results indicate that the cracks generated after the electric pulse breakdown of rock are predominantly tensile, with a hackly pattern, and propagate to significant depths. When shear cracks form inside the rock, they predominantly propagate as small crack growths, resulting in a larger area of the rock being affected by the cracks. The pentagonal prism-shaped HVED exhibited the highest average standard deviation in rock-breaking performance, indicating the greatest variability. Cylindrical and conical HVEDs showed better rock-breaking performance with deeper fractures, though concave surfaces were observed in the center of the fractures. Triangular and quadrangular prism-shaped HVEDs demonstrated the most consistent rock-breaking quality. In addition, increasing the electrode bit diameter reduced the maximum electric field strength within the rock, increased the average electric field strength, and expanded the breaking range. This study provides valuable insights for the development of electric pulse rock-breaking tools and the advancement of HVEP drilling technology.
A filamentous phage promotes meningococcal colonization by selecting hyperadhesive pili variants linking phage infection to bacterial virulence
Authors: Bille Emmanuelle , Mouville Clémence , Brizard Antoine , Wuckelt Morgane , Montabord Melanie , Lécuyer Hervé , Meyer Julie , Jamet Anne , Durel Beatrice , Izabelle Charlotte , Nassif Xavier , COUREUIL mathieu 
Published: January 2025

Abstract

Filamentous phages belong to Inoviruses, a family of non-lytic phages that are mutually beneficial to their bacterial hosts. These phages have a role in bacterial dissemination, biofilm formation or immune evasion. In Neisseria meningitidis, the strains harbouring the MDA filamentous phage are associated with invasive diseases through the MDAΦ key role in epithelial cell colonisation. Using MDAΦ and N. meningitidis as model organisms, we aimed to understand the relationship between filamentous phage infection, type IV pili antigenic variation and bacterial colonisation. The paradigm of filamentous phage infection has been defined for the phages Ff and CTX, where binding to the tips of bacterial type IV pili is a means of infecting their bacterial host. In contrast, we showed that MDAΦ binds N. meningitidis type IV pili along the length of the filament, rather than at the tip, with preferential binding to positively charged variants of PilE (the major fibre-forming pilin) demonstrating a role for antigenic variation in phage infection. Strikingly, bacteria expressing the more positively charged PilE were the most adhesive, meaning that MDAΦ primarily target the most adhesive bacteria. Finally, we showed that adhesion to human cells is sufficient to amplify the phage-positive meningococcal population. Taken together, this study reveals how the propagation strategy of a filamentous phage, aimed at selecting the best coloniser as a host, can promote the selection of hyperadhesive bacterial variants, linking phage infection to bacterial virulence.
Trust and the Future of Learning: A Model for AI-Supported Edtech in Higher Education
Authors: Asare Andy , Yap Robin 
Published: January 2025

Abstract

The current study sought to investigate behavioral factors that affect students’ trust perception in using AI-supported Edtech in a postsecondary institution. A model and research hypotheses were developed based on extensive literature and theoretical reviews on technology acceptance theories to affirm the nature of the hypotheses for the study. The Theory of Planned Behavior (TPB) was adopted as the model for the current study by expanding it with trust, and perceived risk. Data was collected from students at the studying at a public institution in Ontario, Canada. The data collected were analyzed using structural equation modelling (SEM) techniques to affirm the study hypotheses. The findings revealed that all the independent variables of Perceived Compatibility, Ease of Use, Usefulness, Facilitating Condition, Risk, and Subjective Norms had a significant and positive influence on students' Trust perception in using AI-supported Edtech. By examining the influential factors of students' behavioral intention to use Edtech, this study will practically position academicians and policymakers in higher educational institutions in their quest to invest in AI-supported Edtech to prioritize variables aimed at influencing students' trust perception to use AI-supported Edtech.
Interannual Variability of Annual Minimum Sea Surface Temperature in the Eastern China Seas
Authors: Ding Wenxiang , Zhang Caiyun , Chen Yongxin , Mo Jingrui , Zeng Rui , Wu Qiong 
Published: January 2025

Abstract

An understanding of ocean temperature variability and extremes is a prerequisite for assessing the resilience of marine ecosystems. While significant attention has been paid to the impacts of extremely high temperatures, extremely low temperatures have received comparatively less consideration. This study investigated interannual variability in the annual minimum sea surface temperature (Tmin) in the Eastern China Seas from 1985 to 2022. The results reveal significant spatial and temporal patterns in Tmin and its timing. Distinct regional differences were observed between warming areas influenced by warm currents and cooling areas dominated by coastal currents. Both regions showed positive correlations between Tmin and annual minimum air temperature (AirTmin), as well as between the occurrences of Tmin AirTmin, and annual minimum wind (V). However, key regional differences were also observed. From December through March, Tmin was closely linked to air temperature and longwave radiation in warming regions. In cooling regions, Tmin was influenced by wind (V), latent heat flux (QLH) and sensible heat flux (QSH), with December conditions affecting Tmin and February conditions impacting Tmin timing. The study also showed that low Tmin values led to increased chlorophyll levels and a high likelihood of marine cold spells. These findings offer important insights into the dynamics of regional sea surface temperature and help to understand the effects of climate change on marine ecosystems.
Green Synthesis and Assessment of Antibacterial and Antiobiofilm Activities of Silver Nanoparticles Using Carthumas tinctorius Extract
Authors: Hamid Marwa T. , Hussein Nehia N. 
Published: January 2025

Abstract

Silver nanoparticles (AgNPs) synthesized using Carthamus tinctorius flower extract exhibit significant biomedical potential, particularly for their antibacterial and antibiofilm properties. Various characterization techniques, including UV-visible spectroscopy, SEM, TEM, zeta potential, FTIR, and XRD, confirmed the successful biosynthesis of AgNPs with spherical shapes, sizes ranging from 20 to 60 nm. The UV-visible spectrum displayed a peak at approximately 417 nm, indicating AgNP formation.Invitro antimicrobial activity of biosynthesized AgNPs was demonstrated against Staphylococcus haemolyticus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii across different concentrations (100 to 6.25 µg/mL). Results showed concentration-dependent bacterial inhibition, with the highest efficacy observed at 100 µg/mL, especially against E. coli and A.baumannii(inhibition zones of 16.66 mm and 16.33 mm, respectively). The study highlights the potential application of AgNPs as bioactive agents in biotechnology and pharmacology.
Lactobacillus paracasei LCQ-1 Exhibits Good Safety and Effectively Prevents Escherichia coli K99-Induced Diarrhea in Mice
Authors: Wang Fengjie , Chen Li , Li Yang , Gao Guangping , Wang Yanan , Shi Ruili , Zhang Hongjun , Chen Yingyu , Cao Rui , Shi Qiumei , Wu Tonglei 
Published: January 2025

Abstract

Escherichia coli is a common pathogen causing diarrhea in livestock and poultry, posing a serious threat to intestinal health and leading to significant economic losses in the animal husbandry industry. While antibiotics have been used to treat Escherichia coli diarrhea, their numerous drawbacks have led to increasing attention on probiotic interventions. In this study, Lactobacillus paracasei LCQ-1 was isolated from fresh cow dung, and its in vitro and in vivo safety and probiotic properties were evaluated. In vitro experiments demonstrated that Lactobacillus paracasei LCQ-1 exhibited good antimicrobial activity against Escherichia coli K99, along with key probiotic characteristics such as acid production, acid and bile salt tolerance, high-temperature resistance, hydrophobicity, and cell adhesion. Furthermore, it showed no hemolytic activity, gelatinase activity, or mucin-degrading activity, and it only exhibited decarboxylase activity for histamine. While resistant to certain antibiotics, no significant resistance genes were detected, indicating favorable probiotic and safety profiles. In vivo, acute toxicity tests in mice revealed no signs of acute poisoning after 14 days of high-dose oral administration. A 28-day oral toxicity test showed that different doses did not affect organ indices, cause bacterial translocation, alter blood biochemical parameters, or damage the ileum. In the infection protection experiment, oral administration of Lactobacillus paracasei LCQ-1 significantly increased the survival rate of Escherichia coli K99-infected mice from 50–80%. In conclusion, Lactobacillus paracasei LCQ-1 demonstrated excellent safety and probiotic properties both in vitro and in vivo, and shows promising potential in preventing and treating Escherichia coli K99 infections, with possible applications in animal health management.
In silico bioprospecting of the Neotropical Plant Mandacaru (Cereus) for antimicrobial property
Authors: Teodoro João Alfredo , Senra Marcus V. X. , Amaral Danilo T. 
Published: January 2025

Abstract

The mandacaru is a cactus species complex widely known in Brazil, with extensive applications in medicinal, food, and agricultural fields. Although it is used medicinally, by traditional populations, to treat several diseases, knowledge about its biomolecules of biotechnological potential is still limited, specifically regarding antimicrobial and healing properties. The bacterial resistance to conventional antibiotics presents a significant challenge in modern medicine. In light of this scenario, the bioprospecting of mandacaru for biotechnological applications as an antimicrobial begins as a new and imperative research area. In this study, transcriptomic data from three Cereus species (C. fernambucensis, C. hildmannianus, and C. jamacaru) were combined with bioinformatic approaches, including protein modeling, molecular docking, and molecular dynamics simulations, to identify proteins with therapeutic potential for treating wound infections. Our findings highlighted peptides with particularly promising antimicrobial agents, demonstrating efficacy against a range of pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi. Those peptides showed strong interactions with the STD and Na ligands, though the STD ligand emerged as the most likely candidate for enhancing antimicrobial activity. Molecular dynamics revealed that while CF15 exhibited limited stability, CF267, CF48, CH167, and CH176 displayed superior stability, positioning it as the most promising candidate for further investigation. Future work will focus on synthesizing them and evaluating its antimicrobial properties through in vitro and in vivo analyses, with the goal of developing it into a potent therapeutic agent.
A lightweight methodology for Motor Imagery EEG classification utilizing step scaled wavelet fractals and Bi-LSTM architecture
Authors: . Balendra , Sharma Neeraj , Sharma Shiru 
Published: January 2025

Abstract

Brain-Computer Interface (BCI) represents a cutting-edge area of research that integrates neuroscience, engineering, and computer science to establish direct communication links between the human brain and external systems. BCI-based technologies hold significant promise, particularly in the development of prosthetic devices. However, the practical application of BCI in real-time scenarios faces several obstacles, including bulky models, noise interference, artifacts, and the complexity of motor imagery (MI) electroencephalogram (EEG) data, which exhibits both inter-subject and intra-subject variability. To address these challenges, the proposed algorithm utilizes loading and pre-processing of MI EEG, extracts their common spatial patterns, calculates the continuous wavelet transform (CWT) coefficients, computes their proposed step scaled mean wavelet fractals which exhibits robustness towards inherent noises and artifacts, calculates the cross-correlation matrix at different scale for all channels and observes the evolution in cross-correlation matrices with the help of customized Bi-long-short term memory (Bi-LSTM) neural network to classify MI EEG. The customized Bi-LSTM architecture had the size < 10MB showing the effectiveness of methodology for MI EEG classification utilizing embedded based devices. The best classification accuracies achieved with proposed step scaled mean wavelet fractals were 87.75% and 85.45% for intra-subject as well as 76.16% and 71.76% for inter-subject on BCI Competition IV 2b and 2a respectively; the comparative analysis with earlier state of the art methods showed an average improvement of 2.28% and 2.33% for intra-subject as well as 0.22% and 3.21% for inter-subject in accuracy.
Synthesis, characterization, in vitro antibacterial and in vivo antimalarial studies of arylsulphonamoyl ala-val carboxamide dipeptides
Authors: Adoga Ogakwu Sunday , Okoro U. C. , Dattatraya Dethe H. , Shukula Manmohan , Makatini Maya M. 
Published: January 2025

Abstract

The synthesis of twenty-three (23) new ala-val dipeptides carboxamides bearing the sulphonamide functionality are reported. This was achieved by first reacting substituted and unsubstituted benzene sulphonyl chlorides with alanine to give three substituted and unsubstituted benzene sulphonamoyl alkanoic acids (1a-c). Secondly, boc-protected valine carboxamide (carbamate) derivatives (2a-h) were prepared by reacting boc-valine with various primary and secondary aliphatic amines as well as with alicyclic and aromatic amine heterocycles aided by peptide coupling reagents. The carbamates were deprotected and the free amines reacted with the already prepared substituted and unsubstitutedbenzenesulphonamoyl alkanoic acid derivatives aided by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/hydroxybenzotriazole (peptides coupling reagents) to give the substituted and unsubstituted ‘ala-val’ dipeptide carboxamide derivatives bearing the sulphonamide functionality (3a-w). All the synthesized compounds were characterized using FTIR, 1H and 13C-NMR and ESI- and APCI- MS, and the spectra of the compounds were in agreement with the assigned structures. The In vitro antimicrobial study revealed that most compounds were active against the tested organisms. Compounds 3c and 3d were observed to be the most active against E. coli (G-)/Staphylococcus aureus (G+) and trypanosome at zone of inhibition (29 mm) and minimum inhibitory concentration (MIC) of 6.25 and 3.125 µg/mL respectively. The compounds showed moderate to good in vivo antimalarial activity, and compound 3t exhibited the highest percentage (%) inhibition of 73.81. Compound 3s showed the highest % parasitemia (29.55) compared with the 36.96% parasitemia for the negative control.
Role of METTL3 protein in asthma: Insights from transcriptomic profiling and molecular docking analysis
Authors: Jiang Kaichong , Li Qiao , Duan Ling , Zhu Xieying , Wu Shuang 
Published: January 2025

Abstract

Introduction
Asthma is a chronic inflammatory disease characterized by complex immune dysregulation. This study aims to identify key m6A-related genes, particularly METTL3, as potential biomarkers and therapeutic targets in asthma.
Methods
Transcriptomic data from the GEO (GSE134544) dataset were analyzed to identify differentially expressed m6A-related genes. Functional enrichment analysis was performed using clusterProfiler, immune infiltration profiling was conducted with CIBERSORT, and a competing endogenous RNA (ceRNA, including miRNA and lncRNA) network was constructed. Drug enrichment analysis was carried out using DSigDB, and molecular docking was utilized to assess the interaction between Dabigatran and the METTL3 protein.
Results
From 192 differentially expressed genes, four m6A-related genes (METTL3, HNRNPC, IGFBP2, and RBMX) were identified as hub genes. Gene Ontology (GO) analysis revealed significant enrichment in biological processes related to RNA metabolic processes and post-transcriptional regulation, while KEGG analysis identified important pathways such as spliceosome and p53 signaling pathways. METTL3 and HNRNPC were central in the ceRNA network, interacting with miRNAs such as hsa-miR-93-3p and lncRNAs like LINC01529. Drug enrichment analysis identified Dabigatran as a potential METTL3 inhibitor, with molecular docking confirming a stable binding affinity (-5.9 kcal/mol).
Conclusion
This study emphasizes the critical role of m6A-related genes, particularly METTL3 and HNRNPC, as biological macromolecules in asthma pathophysiology, and provides insights into their potential as biomarkers and therapeutic targets for asthma treatment.
Long-term survival of extranodal NK/T-cell lymphoma: a single-center real- world study of 408 cases in China
Authors: Wang Jie , Yang Yunfan , Zhang Chunlan , Zhong Xushu , Liu Qinyu , Xu Caigang 
Published: January 2025

Abstract

Aims
To investigate the long-term survival of extranodal NK/T-cell lymphoma (ENKTL) before the era of new drugs.
Methods
This retrospective real-world study enrolled patients with ENKTL at xx hospital between January, 2012 and December, 2017. Survival analysis and multivariate Cox proportional hazard regression analysis were performed.
Results
Among 408 patients with ENKTL, the 5-year overall survival (OS) was 75.3%, and 5-year progression-free survival (PFS) was 75.3%. Nasal type had significantly better 5-year OS (78.8%) and PFS (78.8%) compared to extranasal type (45.1% and 45.6%, respectively; P < 0.001). Early-stage patients had higher 5-year OS (84.5%) and PFS (91.2%) with chemotherapy plus radiotherapy compared to chemotherapy alone (56.7% and 85.9%, respectively; P < 0.001). Asparaginase-based regimens improved outcomes, especially in advanced stages. Achieving complete remission (CR) after first-line treatment significantly improved 5-year OS (81.9%) and PFS (82.0%). Multivariate analysis showed that Eastern Cooperative Oncology Group (ECOG) (HR = 2.728, 95%CI: 1.563–4.761); type of first-line treatment, including chemotherapy and radiotherapy (HR = 0.303, 95%CI: 0.184–0.501), chemotherapy and ASCT (HR = 0.373, 95%CI: 0.162–0.857), and first-line treatment achieved CR (HR = 0.565, 95%CI: 0.364–0.877), were independent prognostic factors for patients’ OS.
Conclusions
Different treatment strategies might impact the long-term survival of patients with ENKTL before the era of new drugs. Chemotherapy combined with radiotherapy, asparaginase-based regimens, and achieving complete remission (CR) after first-line treatment are associated with improved outcomes, particularly in early-stage and advanced-stage patients. Moreover, ECOG performance status, type of first-line treatment were independent prognostic factors for OS.