bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vaca Muerta Characterization in the Parva Negra Oeste Block, Neuquén, Argentina
Authors: Nawratil Alejandro Enrique , Bustos Crescentino Gabriela Inés , Serra Fernando Adrián 
Published: March 2023

Abstract
The main objective of this study is to evaluate the development of the Vaca Muerta Formation in Parva Negra Oeste Block through horizontal wells. To perform this, two vertical wells drilled recently by Capex were characterized. All information obtained was integrated, including well logs, rock data such as cuttings, sidewall cores and core samples and also 2D/3D seismic.
The first step was oriented to classify the Vaca Muerta Formation into electrofacies using a commercial software. As a result of this, four electrofacies were defined. Later, a geological characterization of the electrofacies was performed using lab data such as geochemical, mineralogical, petrographic and geomechanical studies. A structural and stratigraphic framework was already available from seismic interpretation. Finally, all this information was integrated to the seismic data in order to further understand their vertical and areal distribution in the Block.
This study allowed us to define four electrofacies for the Vaca Muerta Formation in the Parva Negra Oeste Block from well logs and lab data obtained from two vertical wells drilled during the last two years by Capex. These electrofacies show the following vertical variation from base to top of the studied interval: a) organic mudstones, b) silty mudstones, c) radiolaritic mudstones with high porosity and high total organic content, and d) silty fossiliferous mudstones with low porosity and low total organic content. Ultimately, the integration of the geological characterization, a geomechanical model and the seismic interpretation allowed us to define a landing zone for future development of the Block based on hard data provided by well logs, rock information and geological – seismic interpretation.
Prior drilling and subsequent well characterization allowed us to extend the knowledge of the northwestern zone of the Neuquén Basin, which is not densely drilled, adding high quality data, and to evaluate the possibilities of a future development of the Block through horizontal wells.
From Exploration to Development: The Completion Evolution in Vaca Muerta
Authors: Bonapace Juan Carlos , Riolfo Luis Atilio , Zrain Rodrigo , Alric Facundo Gabriel , Moussi Mohamed Lamine , Santander Jorge Nicolas 
Published: March 2023

AbstractOperators in the Neuquén basin have developed Vaca Muerta with different results. In our case, it has gone from vertical exploratory wells, almost ten years ago, to a current development phase with multi-horizontal well PADs targeting different landings. During this period, many lessons have been learned and applied. This paper explains how we have become more efficient and cost effective by increasing production and reducing well delivery cycle time, while fostering the long-term sustainability of the project by reducing the environmental impact of operations.The implementation of different surveying technologies (ranging from initial vertical wells with simple and specialized logs, cores, XRD, proppant tracers and micro seismic interpretation, to multi-landing horizontal stimulated wells with more complex techniques such as 4D seismic, chemical tracers and fiber-optics studies) has guided the evolution of completion design, shifting stimulation jobs towards larger, more complex and more resource consuming scenarios.In this regard, together with the increasing lateral length of the wells, operations have shifted towards technologies that foster time maximization in a cost-effective manner. Water transfer and sand logistics have been reformulated to a new scale. Also plug and perf, coiled tubing and fracturing jobs have boosted performance and reliability through the implementation of new technologies and optimization of the operations sequence.Different field tests and studies have driven changes in completion design to maximize production at lower costs. Some fracture parameters evaluated have shown a positive impact on production. Product QC (e.g., proppant and fluids tests) and service reliability improvements have shown to be key to achieve completion objectives in a timely manner. Great efforts have been made to break paradigms regarding water and sand logistics. Only the combination of all these factors, coupled with the implementation of state-of-the-art technologies, can create a synergy that promotes the sustainable development of unconventional resources.Over the last years, several studies and field tests have shifted the way of conceiving best stimulation practices for unconventional wells. This document describes how the increasingly demanding requirements of completion design were caught up by operations. The historical evolution described in this work shows both positive and negative experiences. Finally, we examine a possible stimulation strategy path for the challenging context that shale developments shall face.
2PSLAF: Privacy-Preserving Secure lightweight authentication framework for Vehicle to Vehicle communications in eHealth Environments
Authors: Rao P Muralidhara , Saraswathi Pedada 
Published: March 2023

Abstract
Internet of Vehicular Things (IoVT) intended to connect vehicles in urban and rural areas to provide communication among the vehicles. With the rapid development of eHealth, the autonomous and intelligent vehicles play a vital role. Now a days, millions of vehicles equipped with smart sensors, devices, and communication facilities for providing quality of service at emergency. Due to network openness, unfortunately, IoVT is subject to a large number of potential issues related to security and privacy. This paper proposes an efficient privacy-preserving secure vehicle-to-vehicle (V2V) communication. Firstly, we propose a privacy-protected identity-based mutual authentication. Further, to enhance the security levels, the session key is derived from timestamps, a common key shared by the vehicles, privacy-protected identity and random nonce decided by both the vehicles. The security analysis shows our scheme withstands several potential attacks, including unknown key share attacks, replay attacks, and key-compromised impersonation attacks. The empirical results show the efficiency of the proposed scheme is computationally taking less time.
Remdesivir Treatment alters Circulating MicroRNA Profile in Coronavirus Disease 2019 (COVID-19) Patients
Authors: Fayyad-Kazan Mohammad , Makki Rawan , Homsi Mahmoud El , Samadi Ahmad , Chaaban Hilal , Majzoub Rania El , Hamade Eva , Fayyad-Kazan Hussein , Badran Bassam 
Published: March 2023

Abstract
Coronavirus disease 2019 (COVID-19), a serious infectious disease caused by the newly discovered Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), represents a significant global health crisis nowadays. Although no specific antiviral drug has been proven to be fully effective against COVID-19, Remdesivir (GS-5734), a nucleoside analogue prodrug, showed beneficial effects upon treating severe hospitalized COVID-19 cases. The molecular mechanism underlying this beneficial therapeutic effect is still vague. In this study, we assessed the effect of Remdesivir treatment on the signature of circulating miRNAs in the plasma of COVID-19 patients. MiRCURY LNA miRNome qPCR Panels were used to characterize the miRNA signature. Individual quantitative real-time RT-PCR (qRT-PCR) was performed to confirm the outcome of miRCURY LNA miRNome qPCR Panels. Our results revealed that Remdesivir can restore the expression of a panel of miRNAs being upregulated in COVID-19 patients into levels comparable to those exhibited by healthy donors. Bioinformatics analysis revealed that these miRNAs are involved in diverse biological processes such as Transforming growth factor beta (TGF-β) signalling pathway, Hippo signaling, P53 signalling and Rap1 signaling pathway. On the other hand, 3 miRNAs (hsa-miR-7-5p, hsa-miR-10b-5p, hsa-miR-130b-3p) were upregulated in patients only following Remdesivir treatment or in naturally remitted patients. These upregulated miRNAs could serve as biomarkers of COVID-19 remission. The output of this study highlights that Remedisivir therapeutic potential involves miRNA-regulated molecular mechanisms.
An Economic Low-profile Elliptical Microstrip Antenna-to-RWG Transition for Microwave Laboratory and X-Band Applications
Authors: Elfergani Issa , Varshney Atul , Sharma Vipul , Kannan S. Rajesh , Udaiyakumar R. , Zebiri Chemseddine , Desai Arpan , Rodriguez Jonathan , Abd-Alhameed Raed 
Published: March 2023

Abstract
The work presents a low-cost compact microstrip line (MSL)-to-rectangular waveguide (RWG) transition for space communication, satellite communication, RADAR, amateur radio; microwave laboratory X-band applications. A 90˚ MSL-to-RWG transition comprises a MSL and a RWG (WR-90). An elliptical microstrip antenna is used as an RF energy launcher in RWG which is fabricated on a low-cost lossy FR4 epoxy substrate with a loss tangent of 0.02 and a permittivity of 4.4. An impedance mismatch between the 50 Ω MSL and 500 Ω RWG is a challenging task and it has been achieved by changes in the probe geometry, microstrip line (width and length) and by adjusting the dimensions (height, width, and length) of the waveguide slot cut at the bottom end. The impedance matching results in an improved -10dB return loss (RL) with insertion loss (IL) of 2.1 dB at 10.12 GHz and a relative bandwidth of approximately 27% (9.84 GHz-10.38 GHz). An extremely unidirectional with directivity (9.97 dBi) and high gain microstrip antenna (8.24 dBi) has been utilized for electromagnetic energy launch into the WR-90. Therefore the designed MSL-to-RWG transition is a suitable candidate for X-band applications such as microwave laboratory experiment purposes, front end in transmitter/receiver of Space and Satellite applications, RADAR, amateur radio, as a connector for MMIC/MIC circuits and devices. The transition-measured results are also presented.
Comparative Analysis of Open and Short Defects in embedded SRAM using Parasitic Extraction Method for Deep Submicron Technology.
Authors: Maddela Venkatesham , Sinha Sanjeet Kumar , Parvathi Muddapu , Sharma Vinay 
Published: March 2023

Abstract
The technology advances from the micron level to the Nanometer level. This striking change in the technology with so many factors might influence the embedded device design and its performance. In the fast-growing technology, it is very difficult to find suitable algorithms to test embedded SRAM. It is noticed that while going to deep sub-nano technologies, the existing test methods may not fully satisfy the test results due to the increased number of faults and defects. Scale-down technologies have an impact on the parasitic effects, creating an additional source of faulty behavior, and making the existing test techniques less effective in detecting them. In this paper we propose a new method, taking the parasitic effect into the consideration, which gives the fault information along with its location. In the proposed method we have considered node-to-node open and short defects for different technologies (45nm, 32nm, and 7nm). It is observed that the proposed test method gives 100% fault coverage which is independent of technology variation.
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis
Authors: Zhao Yang , Teng Hongqi , Deng Yalan , Sheldon Marisela , Martinez Consuelo , Zhang Jie , Tian Annie , Sun Yutong , Nakagawa Shinichi , Yao Fan , Wang Hai , Ma Li 
Published: March 2023

Abstract
MALAT1, one of the few highly conserved nuclear long noncoding RNAs (lncRNAs), is abundantly expressed in normal tissues. Previously, targeted inactivation and genetic rescue experiments identified MALAT1 as a suppressor of breast cancer lung metastasis. On the other hand, Malat1-knockout mice are viable and develop normally. On a quest to discover new roles of MALAT1 in physiological and pathological processes, we found that this lncRNA is downregulated during osteoclastogenesis in humans and mice. Notably, Malat1 deficiency in mice promotes osteoporosis and bone metastasis, which can be rescued by genetic add-back of Malat1. Mechanistically, Malat1 binds to Tead3 protein, a macrophage-osteoclast–specific Tead family member, blocking Tead3 from binding and activating Nfatc1, a master regulator of osteoclastogenesis, which results in the inhibition of Nfatc1-mediated gene transcription and osteoclast differentiation. Altogether, these findings identify Malat1 as a lncRNA that suppresses osteoporosis and bone metastasis.
Lightweight Linguistic Algorithm with Key Exchange Method To Protect User Information In Mobile Ad Hoc Networks (MANET)
Authors: Soni Ashish Kumar , Gupta Rajendra , Khare Ankur 
Published: March 2023

Abstract
Some information is more confidential for every user. The user doesn’t want any type of leakage of private information. The user requirements are the topmost priority of secure communication or information sharing confidentiality with high-tech devices and advanced security systems for protecting non-sharable information to authorized persons. These high-security requirements are open and the development of the best security system for securing user’s data. Nowadays telecommunication fields are advancing rapidly in wireless technology and Mobile Ad hoc Network is the most researched area in wireless technology, and this will be approached in terms of the light weight technology revolution. MANET has one thing of less infrastructure and dynamic behavior of the demanding network strategies which is implemented in provided efficient communication. Encrypted methods and key exchange protocols can be made sure for Man in middle attacks. These Linguistic algorithms with the key exchange method (DH-KELA) are added and work together. The key exchange protocol is working for Man in the middle attack and the Linguistic algorithm performs for information hiding according to the key exchange method with a different pattern. We aim to facilitate double security topology in communication channels before sending confidential user information. It is a piece of more secure and reliable information-hiding concept. This paper is described a Linguistic algorithm with a key exchange method of the Diffie-Hellman (DH), Security Attacks, and security challenges on Mobile Ad-hoc networks.
The Impact of Economic Growth, Population, and Energy Consumption on Environmental Degradation: Evidence from OECD Countries
Authors: Golpîra Hêriş , Sadeghi Heibatolah , Magazzino Cosimo 
Published: March 2023

Abstract
This research aims to examine the validity of the Environmental Kuznets Curve (EKC) hypothesis for the environmental degradation measured by Carbon dioxide emission with the role of renewable and non-renewable energy consumption, Gross Domestic Product (GDP), and the population in 37 Organisation for Economic Co-operation and Development (OECD) countries. The research investigates the period from 1960 to 2019 using panel and individual data methodologies. The panel data analysis shows that the inverted N-shaped cubic polynomial model is preferable to the linear and quadratic models. Unlike the GDP, the positive impacts of the parameters fossil fuel use, renewable consumption, and population are affirmed. In the analysis of individual OECD countries, it is found that the pattern of 38% of the countries is consistent with the pattern resulting from the panel data analysis. While the inverted U-shaped EKC pattern is accepted only by 19% of the countries. Almost all countries with high GDP per capita follow inverse patterns (inverted U or inverted N). This pattern is true regarding the consumption of fossil fuels. Almost all the countries with higher renewable energy consumption follow the N-shaped pattern. This is while the countries with lower GDP per capita follow the U- or N-shaped patterns.
The Effect of Static Magnetic Field of Different Strength and Polarity on Cytokine Production of Human Lymphocytes
Authors: Turuntaš Vladimir , de Luka Silvio , Ristić-Djurovic Jasna L. , Ćirković Saša , Djordjevich Drago , Ristić Siniša , Lalović Nenad , Joksimovic Bojan , Marić Veljko , Lazić Bratislav , Vasilijić Saša , Trbovich Alexander M. 
Published: March 2023

Abstract
Static magnetic fields (SMF) have not been the subject of many investigations, in contrast to electromagnetic fields, regarding their potential health consequences. The majority of them focus on acute, high-strength SMF, measured in multiple tesla (T) units, and attempt to explain the negative health effects of exposure to magnetic resonance imaging. For effects of long-term, moderate strength SMF, in the milliTesla (mT) range, especially on immune cells, there are just a few studies. Upward and downward oriented magnetic poles may have various biological consequences in vitro. However, nobody has looked into this yet. Only the pole with the upward orientation was used in all prior studies. Antigen-presenting cells (APC) and T lymphocytes interact intricately to trigger an immune response. There are no in vitro SMF studies that we are aware of, that used T lymphocytes activated by dendritic cells (DC) as the primary APC. This study aimed to investigate the effects of long-term exposure of moderate strength SMF of different orientations on in vitro cytokine production in cultures consisting of purified human T lymphocytes and human monocyte-derived DC. Cultures of allogenic purified T lymphocytes and human monocyte-derived DC induced to mature by TLR3 and TLR7 agonist were continuously exposed to 1 mT or 56 mT, the upward and downward oriented pole of SMF. The samples were from healthy donors. Supernatants were assayed to quantify IFN-γ, IL-4, IL-17, TNF-α, TNF-β, IL-1 β, IL-6, IL-8 and IL-10 by ELISA. Upward oriented 1 mT and 56 mT SMF significantly increased release of IL-17 (110%; p<0,05) and IFN-γ (40%, p<0,5), respectively, in immature DC culture supernatants. In contrast, upward oriented 1 mT SMF significantly decreased TNF-β production (80%; p<0,05) when we stimulated T cells with immature DC. The IL-4, TNF-α, IL-1 β, IL-6, IL-8, and IL-10 are not affected by upward-oriented SMF. Downward oriented 56 mT SMF significantly increased (45%; p<0,05) TNF-α release when we stimulated T cells with mature DC. The production of other cytokines was unchanged by the downward-oriented SMF. This is the first time in literature that has been shown in vitro different biological effects of the upward and downward oriented static magnetic field. Тhis study helped better understand SMF effects on the immune system, and the selective SMF effect on immune response could have potential therapeutic effects in different immune/allergy disorders.
Development of an in vitro plant regeneration protocol for the spotted duckweed, Landoltia punctata.
Authors: K Satheeshkumar P , Sharma Mrityunjay 
Published: March 2023

Abstract
Landoltia punctata is an aquatic, free-floating angiosperm plant with a very high multiplication rate and numerous commercial uses. In vitro plant regeneration protocols are necessary to exploit these plants as biomanufacturing platforms. It is frequently observed that the protocols established elsewhere are not effective when it was adopted by another group of researchers due to various reasons. In an attempt to develop transgenic plants in L. punctata., the present study developed a successful and reproducible plant regeneration protocol using fronds as explants. Gamborgs’s B% media and MS media were used in the protocol. Callus induction was obtained on Gamborg’s B5 media supplemented with BAP (1 µM), Dicamba (20 µM), and sucrose (2%). For callus growth MS medium with 2, 4-D (30 µM), TDZ (1 µM), and sorbitol (2%) were used. MS medium supplemented with TDZ (15 µM) and the carbon source combination of sorbitol and sucrose (2:1) gave efficient plant regeneration in L. punctata. Half-strength basal MS media was used for rooting and the in vitro regenerated plants were successfully transferred to the maintenance solution (half-strength Hutner’s media) within a span of one week. The established protocol will be used to develop transgenic L. punctata plants expressing recombinant proteins.
Socio-economic Baseline for oil-impacted communities in Ogoniland: Towards a Restoration Framework in Niger Delta, Nigeria.
Authors: Sam Kabari , Zabbey Nenibarini , Vincent-Akpu Ijeoma Favour , Komi Gentle , Onyagbodor Peter Oghogho , Babatunde Bolaji Bernard 
Published: March 2023

Abstract

This study documents the socio-economic baselines in selected oil-impacted communities prior to the commencement of the Ogoni clean-up and restoration project. Adopting mixed approach consisting of semi-structured interviews, focus group discussions (FGDs), key informant interviews (KIIs), and household surveys, we surveyed the pre-remediation socio-economic conditions in the Ogoniland communities between July 2018 to March 2019. Results indicated that almost all respondents (99.6%) agreed that the smell of petroleum products or crude oil was evident in the air they breathed even as there were visible black particles (soot) in the respondents’ nostrils, on their clothes and in water. The respondents described the ambient air as smoky, and choked with an offensive smell. The household waters were smelly, brownish, or oily, and most respondents (76%) cannot afford to treat their water. Forty-two percent of the respondents who relied on fishing and farming for a living sought for alternative means of subsistence and acknowledged that oil pollution caused stunted growth and low crop yield. The majority of respondents (91%) reported falling fish catches, while the fish caught smell and taste of oil, lowering their market value and posing a potential health risk to consumers. It is evident that oil pollution has impacted the socio-ecological values and sustainable livelihood in Ogoniland. This study provides a framework for monitoring post-remediation socio-economic improvements in Ogoniland. It also highlights areas of urgent intervention to improve livelihood, and access to basic amenities (e.g., potable drinking water), waste management infrastructure, and statutory policy changes for sustainable development in Ogoniland.