bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetic Resonance Imaging of Mouse Cerebral Cavernomas Reveal Differential Lesion Progression and Variable Permeability to Gadolinium
Authors: Fisher Delaney G. , Sharifi Khadijeh A. , Ulutas E. Zeynep , Kumar Jeyan S. , Kalani M. Yashar S. , Miller G. Wilson , Price Richard J. , Tvrdik Petr 
Published: April 2023

Background:
Cerebral cavernous malformations, also known as cavernous angiomas, are blood vessel abnormalities comprised of clusters of grossly enlarged and hemorrhage-prone capillaries. The prevalence in the general population, including asymptomatic cases, is estimated to be 0.5%. Some patients develop severe symptoms, including seizures and focal neurological deficits, whereas others remain asymptomatic. The causes of this remarkable presentation heterogeneity within a primarily monogenic disease remain poorly understood.

Methods:

We established a chronic mouse model of cerebral cavernous malformations, induced by postnatal ablation of
Krit1
with
Pdgfb-CreERT2
, and examined lesion progression in these mice with T2-weighted 7T magnetic resonance imaging (MRI). We also established a modified protocol for dynamic contrast-enhanced MRI and produced quantitative maps of gadolinium tracer gadobenate dimeglumine. After terminal imaging, brain slices were stained with antibodies against microglia, astrocytes, and endothelial cells.

Results:
These mice develop cerebral cavernous malformations lesions gradually over 4 to 5 months of age throughout the brain. Precise volumetric analysis of individual lesions revealed nonmonotonous behavior, with some lesions temporarily growing smaller. However, the cumulative lesional volume invariably increased over time and after about 2 months followed a power trend. Using dynamic contrast-enhanced MRI, we produced quantitative maps of gadolinium in the lesions, indicating a high degree of heterogeneity in lesional permeability. MRI properties of the lesions were correlated with cellular markers for endothelial cells, astrocytes, and microglia. Multivariate comparisons of MRI properties of the lesions with cellular markers for endothelial and glial cells revealed that increased cell density surrounding lesions correlates with stability, whereas denser vasculature within and surrounding the lesions may correlate with high permeability.

Conclusions:
Our results lay a foundation for better understanding individual lesion properties and provide a comprehensive preclinical platform for testing new drug and gene therapies for controlling cerebral cavernous malformations.
Exercise Mitigates Endothelial Pyroptosis and Atherosclerosis by Downregulating NEAT1 Through N6-Methyladenosine Modifications
Authors: Yang Qingyuan , Chen Shiliang , Wang Xingyi , Yang Xinyu , Chen Liangqi , Huang Tuo , Zheng Yang , Zheng Xianghui , Wu Xiaojun , Sun Yong , Wu Jian 
Published: April 2023

Background:
The benefits of exercise on the cardiovascular system are widely recognized; however, the underlying mechanisms are unknown. Here, we report the effect of the long noncoding RNA NEAT1 (nuclear paraspeckle assembly transcript 1), which is regulated by exercise, on atherosclerosis development after N6-methyladenosine (m6A) modifications.

Methods:

Using clinical cohorts and NEAT1
−/−
mice, we determined the exercise-mediated expression and role of NEAT1 in atherosclerosis. To investigate the mechanism of epigenetic modification of NEAT1 regulated by exercise, we identified METTL14 (methyltransferase-like 14)—a key m6A modification enzyme under exercise—and found that METTL14 alters the expression and role of NEAT1 through m6A modification and elucidated the specific mechanism of METTL14 in vitro and in vivo. Finally, the NEAT1 downstream regulatory network was investigated.

Results:
We found that NEAT1 expression was downregulated with exercise and that downregulation of NEAT1 was an important factor in the improvement of atherosclerosis with exercise. Exercise-mediated loss of function of NEAT1 can delay atherosclerosis. Mechanistically, we showed that exercise induced a significant downregulation of m6A modification and METTL14, which binds to the m6A sites of NEAT1 and promotes NEAT1 expression through subsequent YTHDC1 (YTH domain-containing 1) recognition to promote endothelial pyroptosis. Furthermore, NEAT1 induces endothelial pyroptosis by binding KLF4 (Kruppel-like factor 4) to promote the transcriptional activation of the key pyroptotic protein NLRP3 (NOD-like receptor thermal protein domain-associated protein 3), whereas exercise can attenuate NEAT1-mediated endothelial pyroptosis to improve atherosclerosis.

Conclusions:
Our study of NEAT1 provides new insights into the improvement of atherosclerosis by exercise. This finding demonstrates the role of exercise-mediated NEAT1 downregulation in atherosclerosis while expanding our understanding of the mechanisms by which exercise regulates long noncoding RNA function through epigenetic modifications.
Use of a Pulmonary Artery Pressure Sensor to Manage Patients With Left Ventricular Assist Devices
Authors: Thohan Vinay , Abraham Jacob , Burdorf Adam , Sulemanjee Nasir , Jaski Brian , Guglin Maya , Pagani Francis D. , Vidula Himabindu , Majure David T. , Napier Rebecca , Heywood Thomas J. , Cogswell Rebecca , Dirckx Nicholas , Farrar David J. , Drakos Stavros G. , Burdorf Adam , Nsair Ali , Kolodziej Andrew , Bhimaraj Arvind , Lampert Brent , Jaski Brian , Chien Christopher , Majure David , Pagani Francis , Vidula Himabindu , Herr Jared , Rahman Joseph , Yuzefpolskaya Melana , Zacharias Michael , Jacob Miriam , Sulamanjee Nasir , Moss Noah , Patel Priyesh , Cogswell Rebecca , Napier Rebecca , Drakos Stavros , Heywood Thomas 
Published: April 2023

Background:
Hemodynamic-guided management with a pulmonary artery pressure sensor (CardioMEMS) is effective in reducing heart failure hospitalization in patients with chronic heart failure. This study aims to determine the feasibility and clinical utility of the CardioMEMS heart failure system to manage patients supported with left ventricular assist devices (LVADs).

Methods:
In this multicenter prospective study, we followed patients with HeartMate II (n=52) or HeartMate 3 (n=49) LVADs and with CardioMEMS PA Sensors and measured pulmonary artery pressure, 6-minute walk distance, quality of life (EQ-5D-5 L scores), and heart failure hospitalization rates through 6 months. Patients were stratified as responders (R) and nonresponders to reductions in pulmonary artery diastolic pressure (PAD).

Results:

There were significant reductions in PAD from baseline to 6 months in R (21.5–16.5 mm Hg;
P
<0.001), compared with an increase in NR (18.0–20.3;
P
=0.002), and there was a significant increase in 6-minute walk distance among R (266 versus 322 meters;
P
=0.025) compared with no change in nonresponder. Patients who maintained PAD <20 compared with PAD ≥20 mm Hg for more than half the time throughout the study (averaging 15.6 versus 23.3 mm Hg) had a statistically significant lower rate of heart failure hospitalization (12.0% versus 38.9%;
P
=0.005).

Conclusions:
Patients with LVAD managed with CardioMEMS with a significant reduction in PAD at 6 months showed improvements in 6-minute walk distance. Maintaining PAD <20 mm Hg was associated with fewer heart failure hospitalizations. Hemodynamic-guided management of patients with LVAD with CardioMEMS is feasible and may result in functional and clinical benefits. Prospective evaluation of ambulatory hemodynamic management in patients with LVAD is warranted.

Registration:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT03247829.
Linkage of the Circumglobal Teleconnection and the Interannual Variability of Early Spring Diabatic Heating over Low-Latitude Highlands in Southeast Asia
Authors: Yang Yu , Wen Dayong , Cao Jie 
Published: April 2023

Abstract
This study explores the linkage of the circumglobal teleconnection (CGT) on the variability of early spring diabatic heating over the Southeast Asian low-latitude highlands (SEALLH) using ERA5 data. The early spring diabatic heating over the SEALLH shows significant interannual variability with a quasi-3-yr period. Anomalies in the advection of the early spring diabatic heating in the troposphere over the SEALLH associated with CGT are mainly responsible for the interannual variability of early spring diabatic heating over the SEALLH. When CGT is in phase with an anomalous cyclone over the eastern midlatitude North Atlantic, an anomalous cyclone usually dominates the west SEALLH throughout the troposphere. Stronger-than-normal southerly winds located on the east flank of the anomalous cyclone in the lower–upper troposphere transport more high-enthalpy air mass from lower latitudes to the SEALLH and then result in stronger-than-normal early spring diabatic heating over the SEALLH. When CGT is in phase with an anomalous anticyclone over the eastern North Atlantic, the opposite conditions occur, and weaker-than-normal early spring diabatic heating is observed over the SEALLH. Such significant correlation between CGT and early spring diabatic heating over the SEALLH can persist from winter to early summer. The key physical processes revealed in the observational analysis are mostly confirmed by the historical simulation performed with the EC-EARTH3 model.

Significance Statement
The low-latitude highlands in Southeast Asia are one of the earliest diabatic heating sources in the Asian summer monsoon region. Variability of diabatic heating over the low-latitude highlands in Southeast Asia significantly regulates the weather and climate over the Asian summer monsoon region. However, the interannual variability of early spring diabatic heating over the low-latitude highlands in Southeast Asia remains unclear. This study determines that the circumglobal teleconnection links with the interannual variability of early spring diabatic heating over the low-latitude highlands in Southeast Asia via modulating the local advection process from the previous winter. These results build a bridge connecting the anomalous signals occurring in the upper reaches of the low-latitude highlands in Southeast Asia with the weather and climate in the local and lower reaches of the low-latitude highlands in Southeast Asia.
The Variability of Pan Evaporation over China during 1961–2020
Authors: Wang Hong , Sun Fubao , Liu Fa , Wang Tingting , Feng Yao , Liu Wenbin 
Published: April 2023

Abstract
The most basic features of climatological normals and variability are useful for describing observed or likely future climate fluctuations. Pan evaporation (Epan) is an important indicator of climate change; however, current research on Epan has focused on its change in mean rather than its variability. The variability of monthly Epan from 1961 to 2020 at 969 stations in China was analyzed using a theoretical framework that can distinguish changes in Epan variance between space and time. The Epan variance was decomposed into spatial and temporal components, and the temporal component was further decomposed into interannual and intra-annual components. The results show that the variance in Epan was mainly controlled by the temporal component. The time variance was mainly controlled by intra-annual variance, decreasing continuously in the first 30 years, and slightly increasing after the 1990s. This is mainly due to the fact that the decrease of wind speed and the increase of water vapor pressure deficit with the temperature increase offset each other and inhibit the variability of Epan. The variance decreased more in the northern region, whereas it exhibited a small decrease or slight increase in the southern region. The reduction in seasonality was dominated by spring, followed by summer. The differences in Epan variability in space and season were mainly caused by the differing rates of change in evaporation driving forces, such as a greater reduction in wind speed in the northern region and spring.

Significance Statement
The purpose of this study is to better understand how the variability of evaporation changes rather than in mean under climate change. This is important because the variability is useful to describe the observed or likely future fluctuations, and a small fluctuation may have large impacts on water practices, such as agricultural production. Our findings showed that the temporal and spatial variability of evaporation decreased due to its drivers offsetting each other. However, because the drivers are numerous and continuously changing under climate change, it is necessary to pay attention to its mean and variability for serving water resources practice.
Calibration Transfer Methodology for Cloud Radars Based on Ice Cloud Observations
Authors: Jorquera Susana , Toledo Bittner Felipe , Delanoë Julien , Berne Alexis , Billault-Roux Anne-Claire , Schwarzenboeck Alfons , Dezitter Fabien , Viltard Nicolas , Martini Audrey 
Published: April 2023

Abstract
This article presents a calibration transfer methodology that can be used between radars of the same or different frequency bands. This method enables the absolute calibration of a cloud radar by transferring it from another collocated instrument with known calibration, by simultaneously measuring vertical ice cloud reflectivity profiles. The advantage is that the added uncertainty in the newly calibrated instrument can converge to the magnitude of the reference instrument calibration. This is achieved by carefully selecting comparable data, including the identification of the reflectivity range that avoids the disparities introduced by differences in sensitivity or scattering regime. The result is a correction coefficient used to compensate measurement bias in the uncalibrated instrument. Calibration transfer uncertainty can be reduced by increasing the number of sampling periods. The methodology was applied between collocated W-band radars deployed during the ICE-GENESIS campaign (Switzerland 2020/21). A difference of 2.2 dB was found in their reflectivity measurements, with an uncertainty of 0.7 dB. The calibration transfer was also applied to radars of different frequency, an X-band radar with unknown calibration and a W-band radar with manufacturer calibration; the difference found was −16.7 dB with an uncertainty of 1.2 dB. The method was validated through closure, by transferring calibration between three different radars in two different case studies. For the first case, involving three W-band radars, the bias found was of 0.2 dB. In the second case, involving two W-band and one X-band radar, the bias found was of 0.3 dB. These results imply that the biases introduced by performing the calibration transfer with this method are negligible.
Validity and Reliability of Turkish Version of the Munich Wrist Questionnaire in Patients With Wrist Problems
Authors: Yetiş Mehmet , Ceylan İsmail , Canlı Mehmet , Gürses Ömer Alperen , Aslan Mensure , Horoz Levent , Tayfur Abdulhamit 
Published: April 2023

Evidence for the validity and reliability of the Turkish version of the Munich Wrist Questionnaire (MWQ), a patient reported outcome measurement tool (PROM) was evaluated. A total of 80 patients (54.1 ± 1.4 years, 68 females) with wrist problems were recruited. The MWQ was translated into Turkish (MWQ-TR). Criterion validity with Patient-Rated Wrist Evaluation (PRWE) and Disabilities of the Arm, Shoulder and Hand (DASH) was tested by using Pearson’s correlation coefficients. Intraclass correlations coefficient (ICC) was used to analyze the test-retest reliability. There was a moderate correlation (r = −0.49, p < 0.001) between MWQ-TR and DASH, while correlations were strong between MWQ-TR and PRWE (r = 0.69, p < 0.001). Test-retest reliability of MWQ-TR was moderate (ICC = 0.67, 95% CI 0.26–0.84). The MWQ-Turkish version demonstrated evidence for its validity and reliability to evaluate pain, work/daily life activities and function in people with wrist problems in a Turkish population.
Novel cannulation strategy with a bidirectional cannula for distal limb perfusion during peripheral veno-arterial extracorporeal life support: A preliminary, single-centre study
Authors: Simons Jorik , Doddema Arne R , Körver Erik PJ , di Mauro Michele , Agricola Sandra , Smets Jeroen , Metz Renske , Mariani Silvia , De Piero Maria Elena , Matteucci Matteo , Romeo Jamie , Ravaux Justine M. , van Mook Walther NKA , Mees Barend ME , Lorusso Roberto 
Published: April 2023

Introduction Limb ischemia is a severe complication of peripheral veno-arterial extracorporeal life support (V-A ECLS). Several techniques have been developed to prevent this, but it remains a major and frequent adverse event (incidence: 10–30%). In 2019, a new cannula with bidirectional flow (retrograde towards the heart and antegrade towards the distal limb) has been introduced. A single-centre experience with this cannula in patients undergoing peripheral V-A ECLS is herewith reported. Methods This prospective observational study included adults (≥18 years) undergoing V-A ECLS from January 2021 to October 2022 with the use of a bidirectional femoral artery cannula. Primary outcome was limb ischemia requiring intervention during cardio-circulatory support. Secondary outcomes were compartment syndrome, limb amputation, cannulation site bleeding, need for other surgical intervention due to cannula related complications, duplex ultrasound parameters from the femoral vessels, and in-hospital survival. Results Twenty-two consecutive patients were included. During extracorporeal life support (ECLS) support, limb ischemia requiring intervention occurred in one patient (4.5%) and no patients developed a compartment syndrome, or required a fasciotomy or amputation. Significant bleeding was reported in two patients (9%) due to slight cannula dislodgement, easily solved with cannula repositioning. In-hospital survival was 63.6%. Conclusions The bidirectional cannula is associated with a low risk for limb ischemia-related complications compared to current literature, and apparently represents a safe alternative to dedicated distal perfusion cannula. Further studies are warranted to confirm these preliminary findings.
Development and Validation of a Cognitive Load Measure for General Educational Settings
Authors: Ober Teresa M. , Lu Yikai , Blacklock Chessley B. , Liu Cheng , Cheng Ying 
Published: April 2023

We develop and validate a self-report measure of intrinsic and extrinsic cognitive load suitable for measuring the constructs in a variety of learning contexts. Data were collected from three independent samples of college students in the U.S. ( N total = 513; M age = 21.13 years). Kane’s (2013) framework was used to validate the measure. Three types of validity evidence were presented: scoring, generalization, extrapolation. After establishing evidence of validity, especially measurement invariance, we then compared group mean differences based on students’ demographic characteristics. These findings support the psychometric integrity of this measure of cognitive load, which may be used to investigate cognitive load in various learning contexts, particularly examining factors that may perpetuate or mitigate differences in cognitive load between students. Such a measure could be useful in educational and clinical settings as a mechanism for early identification of potential learning challenges.
Different Concentrations of Albumin Versus Crystalloid in Patients with Sepsis and Septic Shock: A Meta-Analysis of Randomized Clinical Trials
Authors: Geng Li , Tian Xiaoxue , Gao Zifeng , Mao Aiqin , Feng Lei , He Chao 
Published: April 2023

Objective The best type of resuscitation fluids for sepsis and septic shock patients remains unclear. The aim of this study was to evaluate the efficacy of different concentrations of albumin on reducing the mortality rate of theses patients by meta-analysis. Materials and Methods PubMed, EMBASE, and Web of Science databases were used for screening the relevant studies. Randomized controlled trials (RCTs) were eligible if they compared the effects of albumin with crystalloid on mortality in patients with sepsis and septic shock. Data were examined and extracted by two reviewers independently. Any disagreements were resolved by consensus with or without the help from a third reviewer. Data including mortality, sample size of the patients, and resuscitation endpoints were extracted. Meta-analysis was carried based on the corresponding odds ratios with 95% confidence intervals. Results Eight studies with a total of 5124 septic patients and 3482 septic shock patients were included in this study. Compared with crystalloid, the use of albumin may represent a trend toward reduced the 90-day mortality of septic patients (OR 0.91 [0.80, 1.02]; P = .11) and significantly improved the outcome of septic shock patients (OR 0.85 [0.74, 0.99]; P = .04). Further analysis showed a potentially beneficial role of both 4% to 5% and 20% albumin on reducing the mortality of septic patients. The use of 20% albumin significantly decreased the 90-day mortality of septic shock patients (OR 0.81 [0.67, 0.98]; P = .03), which was better than 4% to 5% albumin and crystalloid. Conclusions Albumin treatment, particularly 20% albumin, significantly reduced the 90-day mortality in septic shock patients. Both 4% to 5% and 20% of albumin may work better than crystalloid in improving the survival rate of patients with sepsis, but more relative RCTs are required for validation.
Association of Frailty and Cardiopulmonary Resuscitation Outcomes in Older U.S. Veterans
Authors: Tosi Dominique M , Fernandez Marlena C , Oomrigar Shivaan , Burton Lorena P , Hammel Iriana S , Quartin Andrew , Ruiz Jorge G 
Published: April 2023

Objectives: Determine the association between frailty and immediate survival of cardiopulmonary resuscitation (CPR) in older Veterans. Secondary outcomes: compare in-hospital mortality, duration of resuscitation efforts, hospital and intensive care unit (ICU) length of stay, neurologic outcomes, and discharge disposition between frail and non-frail Veterans. Methods: Retrospective cohort study including Veterans 50 years and older, who were “Full Code” and had in-hospital cardiac arrest between 7/1/2017 and 6/30/2020, at the Miami VAMC. Frailty Index for the VA (VA-FI) was used to determine frailty status. Immediate Survival was determined by return of spontaneous circulation (ROSC) and in-hospital mortality was determined by all-cause mortality. We compared outcomes between frail and non-frail Veterans using chi-square test. After adjusting for age, gender, race, and previous hospitalizations, we used multivariate binomial logistic regression with 95% confidence intervals to analyze the relationship between immediate survival and frailty, and in-hospital mortality and frailty. Results: 91% Veterans were non-Hispanic, 49% Caucasian, 96% male, mean age 70.7 ± 8.5 years, 73% frail and 27% non-frail. Seventy-six (65.5%) Veterans had ROSC, without difference by frailty status ( P = .891). There was no difference based on frailty status of in-hospital mortality, discharge disposition, or neurologic outcomes. Frail and non-frail Veterans had resuscitation efforts lasting the same amount of time. Conclusions and Implications: CPR outcomes were not different depending on frailty status in our Veteran population. With these results, we cannot use frailty – as measured by the VA-FI - as a prognosticator of CPR outcomes in Veterans.