bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia inducible factor‐1 activator munc‐18‐interacting protein 3 promotes tumour progression in urothelial carcinoma
Authors: Ikeda Junichi , Ohe Chisato , Tanaka Noritaka , Yoshida Takashi , Saito Ryoichi , Atsumi Naho , Kobayashi Takashi , Kinoshita Hidefumi , Tsuta Koji , Sakamoto Takeharu 
Published: January 2023

AbstractBackgroundAlthough the introduction of multiple agents targeting immune and oncogenic signaling pathways has substantially changed the treatment strategies for urothelial carcinoma (UC), these drugs still have some limitations, such as adverse effects and limited responses depending on the molecular subtype of UC. Therefore, the development of novel molecular‐targeted therapies is required. Munc18‐1‐interacting protein 3 (Mint3), which specifically activates hypoxia inducible factor‐1 (HIF‐1) during normoxia in cancer and some stromal cells, might be a good target for UC treatment without severe adverse effects. However, the expression and function of Mint3 in UC remain unclear.MethodsWe prepared bladder cancer (n = 117) and upper tract UC (n = 207) cohorts and analyzed the correlation between Mint3 expression in the tissue microarray and the clinicopathological factors/prognosis. To clarify the function of Mint3 in UC cells, control and Mint3‐depleted UC cells were analyzed for HIF‐1 activity, HIF‐1 target gene expression, cell proliferation, glycolysis, motility and invasion in vitro. Tumorigenicity and angiogenesis in control and Mint3‐depleted UC cells were analyzed in mouse xenograft models. A Mint3 inhibitor, naphthofluorescein and gemcitabine were administrated into UC xenograft‐bearing mice.ResultsMint3 was expressed at various levels in UC cells; high expression was correlated with a poor prognosis but not with the molecular subtype of UC in two independent UC cohorts. Mint3 depletion did not affect cell proliferation but attenuated HIF‐1 activity, HIF‐1 target gene expression, glycolysis and motility/invasion in both luminal‐ and basal‐molecular‐subtype UC cells in vitro. Mint3 depletion in UC cells also attenuated tumour growth and angiogenesis in xenograft models. In addition, pharmacological inhibition of Mint3 sensitized chemo‐resistant UC xenografts to gemcitabine.ConclusionsOur findings indicate that Mint3 is a potential target for UC therapy with or without chemotherapy, independent of the molecular subtype of UC.
Fine needle aspiration cytology of metastatic SMARCA4‐deficient sinonasal teratocarcinosarcoma: First report in literature
Authors: Gopakumar Akhila , Kakkar Aanchal , Kaur Kavneet , Sikka Kapil , Thakar Alok , Sharma Atul , Jain Deepali 
Published: January 2023

AbstractInactivating mutations of SMARCA4 and accompanying loss of BRG1 immunoexpression were recently identified in majority of sinonasal teratocarcinosarcomas (TCS). These rare and aggressive neoplasms have potential for nodal metastasis, presenting opportunities for diagnosis on fine needle aspiration cytology (FNAC). However, their cytological features have not been described till date. A 22‐year‐old male was diagnosed to have SMARCA4‐deficient TCS on a nasal mass biopsy, and was started on neoadjuvant chemotherapy. Four months later, FNAC from cervical lymph nodes showed predominantly discohesive tumor cells with moderate to abundant cytoplasm and enlarged vesicular nuclei with prominent nucleoli. Occasional cohesive fragments showed ovoid to spindled tumor cells attached to fibrovascular cores. Few loosely cohesive cells with scant cytoplasm and nuclei having stippled chromatin, and rhabdoid cells were also seen. Frequent mitoses, apoptosis and nuclear streaking were evident. Overt squamous or glandular differentiation was absent. Tumor cells showed loss of BRG1 immunostaining and β‐catenin immunopositivity on a cell block, consistent with metastatic SMARCA4‐deficient TCS. The diversity of cell types in SMARCA4‐deficient TCS can result in a broad spectrum of cytological features that overlap with that of other regional metastatic tumors including neuroendocrine carcinoma, olfactory neuroblastoma and melanoma. Further, all components of TCS as seen in the primary tumor may not be present in nodal metastases. Thus, SMARCA4‐deficient TCS should be considered in the differential diagnosis of metastatic poorly/undifferentiated malignancies in cervical lymph node aspirates, and appropriate ancillary tests viz. BRG1 immunostaining employed for accurate diagnosis.
Synthesis and Catalytic Studies of Thermoresponsive Copper (I) Complex towards Click Reactions
Authors: Gayathri Varnakumar , Stephen K. , Prem Suresh , Ayyadurai Niraikulam , Samanta Debasis 
Published: January 2023

AbstractClick reaction or copper‐assisted azide–alkyne cycloaddition (CuAAC) reaction can conveniently synthesize desired organic molecules or functionalize biological macromolecules. In many cases, trace amounts of residual copper from the reaction mixture are not trivial to remove when the exhaustive purification step is avoided to fulfill the essential criteria of a click reaction. It is often detrimental, particularly for biochemical applications or when it involves biological macromolecules. Herein, we have reported the synthesis of a new type of copper (I) complex as a smart catalyst for click reaction, which can be separated from the reaction mixture very easily by the slight elevation of temperature, thanks to its thermoresponsive behavior. The click reactions using a thermoresponsive catalyst were first studied in an aqueous medium using various organic molecules containing alkyne and azide functional groups. Later, the strategy was extended to biological macromolecules like collagen.
Full‐scale shaking table tests on soil liquefaction‐induced uplift of buried pipelines for buildings
Authors: Ko Yung‐Yen , Tsai Tung‐Ying , Jheng Kun‐Yan 
Published: January 2023

AbstractThe uplift effect induced by soil liquefaction on buried pipelines that are critical to the serviceability of buildings, including potable water, natural gas, and sewage pipelines, was experimentally investigated. A full‐scale physical model of pipelines buried in liquefiable ground comprising distribution mains and service lines that connect distribution mains and buildings was built. Shaking table tests were conducted on this model with actual earthquake records utilized as the input motions. The performance of gravel as pipe ditch backfill for mitigating the liquefaction‐induced uplift was also evaluated. During the tests, accelerometers and piezometers were installed in free‐field (FF) soil and around the pipelines to reveal the pipeline–soil interaction and the soil liquefaction development. Wire potentiometers were also utilized to monitor any uplift or subsidence of the pipelines. Results indicate that the distribution mains for natural gas and sewage experienced an uplift during liquefaction of ground specimen. The excess porewater pressure buildup level around the uplifted pipelines was generally lower than that in FF soil and was even lower on top of the pipeline circumference, which may be attributed to the pipeline–soil interaction. Furthermore, the practicability of an existing simplified model for anti‐uplift stability assessment and its corresponding uplift force estimation were examined based on aforementioned observations. The findings indicate that the uplift effect for large‐diameter pipelines may be underestimated by simply using this simplified model. This research can be used as a reference for the seismic design of buried pipelines, which is beneficial for disaster prevention and mitigation.
Characteristics of sediment particle size distribution in the Pisha sandstone area of the middle Yellow River under the effects of vegetation and complex erosion
Authors: Zhang Pan , Xiao Peiqing , Yao Wenyi , Liu Guobin , Sun Weiying 
Published: January 2023

AbstractPisha sandstone is a soft bedrock composed of sandstone, sand shale and mudstone, with very low diagenesis and erosion resistance. The sources of coarse sediment and the processes of sediment production and transportation in Pisha sandstone areas are currently little understood. Understanding these processes will help us to comprehend the characteristics of sediment particle size distribution on slopes and gullies under the effects of vegetation and complex erosion. This will provide a scientific basis for the control of coarse sediments of the Yellow River and ecological restoration of the Pisha sandstone area. In this study, the process of sediment selectivity and transportation in the slope–gully system was analyzed through soil sampling in different seasons in the Erlaohugou small watershed, China. There is a clear selectivity phenomenon in the process of transporting sediment particles from the slope to the gully, and the coarse sediment particles are transported preferentially from the slope to the gully. There is a good response relationship between the distribution characteristics of sediment particles and the multi‐dynamic interaction. The complex erosion type in different seasons has a significant impact on sediment production and transportation, and most particles were transported under hydraulic power.
Microneedle system for tissue engineering and regenerative medicine
Authors: Zhang Yixin , Xu Yanteng , Kong Huimin , Zhang Jiabin , Chan Hon Fai , Wang Jiasi , Shao Dan , Tao Yu , Li Mingqiang 
Published: January 2023

AbstractGlobal increasing demand for high life quality and length facilitates the development of tissue engineering and regenerative medicine, which apply multidisciplinary theories and techniques to achieve the structural reconstruction and functional recovery of disordered or damaged tissues and organs. However, the clinical performances of adopted drugs, materials, and powerful cells in the laboratory are inescapably limited by the currently available technologies. To tackle the problems, versatile microneedles are developed as the new platform for local delivery of diverse cargos with minimal invasion. The efficient delivery, as well as painless and convenient procedure endow microneedles with good patient compliance in clinic. In this review, we first categorize different microneedle systems and delivery models, and then summarize their applications in tissue engineering and regenerative medicine mainly involving maintenance and rehabilitation of damaged tissues and organs. In the end, we discuss the advantages, challenges, and prospects of microneedles in depth for future clinical translations.
Estimating the value of life expectancy gains in Tanzania using the life satisfaction and model based approaches
Authors: Da Costa Shaun 
Published: January 2023

AbstractPolicymakers in low and lower‐middle income countries often face difficult trade‐offs between saving lives and livelihoods, as exemplified by the COVID‐19 pandemic. Yet, evidence regarding the preferences of the population is often lacking in such settings. In this paper, I estimate the value of an additional year of life expectancy in Tanzania using information on subjective well‐being and population mortality. More specifically, I combine age‐sex specific subnational estimates of remaining life expectancy with data from a representative household survey, which includes information on consumption expenditures and life satisfaction. This information is then carried forward into a life satisfaction regression to estimate the trade‐off between consumption and an additional year of life expectancy. The results imply that a representative individual from the sample would be willing to trade off around 9% of their annual consumption expenditure to obtain an additional year of remaining life expectancy. The estimated values are close to those derived from calibrated models based on different elicitation methods, such as revealed preferences. This suggests that life satisfaction measures could be useful in deriving estimates of the value of longevity changes in environments where traditional methods, such as estimating compensating wage differentials, are difficult to apply.
Real‐world evidence on venetoclax in chronic lymphocytic leukemia: The Italian experience
Authors: Laurenti Luca , Scarfò Lydia , Frustaci Anna Maria , Sanna Alessandro , Iannella Emilia , Caira Morena , Finsinger Paola , Schifano Silvia , Neri Benedetta , Molica Stefano , Mauro Francesca Romana 
Published: January 2023

AbstractChronic lymphocytic leukemia (CLL) is the most common form of adult leukemia in the western world. In Italy, venetoclax was approved for use in patients with CLL as monotherapy in 2017 and in combinations in 2019. As a result of this delayed approval, there are relatively few real‐world studies from Italian clinical practice and much of the data are in heavily pretreated patients. We have collected the available studies in Italian routine practice. Three studies confirm the effectiveness and tolerability of this agent in patients with relapsed/refractory CLL and high‐risk disease characteristics, many of whom had received prior B‐cell receptor signaling treatment. Addition of rituximab to venetoclax produced more complete responses in patients with relapsed/refractory CLL, while higher disease burden and progression while receiving a prior Bruton's tyrosine kinase inhibitor were both associated with poorer outcomes in patients treated with venetoclax. Venetoclax was well‐tolerated with low discontinuation rates. No studies of venetoclax plus obinutuzumab for the first‐line treatment of patients with CLL were available due to the short time since approval in Italy. Several cohorts addressed the impact of COVID‐19 on patient management and outcomes, suggesting that treated patients and those in clinical observation had similar rates of COVID‐19‐related hospital admission, intensive care unit admission, and mortality. Overall, the responses and tolerance to venetoclax observed in the Italian real‐world setting confirm the tolerability and effectiveness of venetoclax regimens in high‐risk patients.
The performance of OECD's composite leading indicator
Authors: Ojo Mustapha Olalekan , Aguiar‐Conraria Luís , Soares Maria Joana 
Published: January 2023

AbstractThis paper evaluated the performance of OECD's Composite Leading Indicator using the Continuous Wavelet Transform. We used various wavelet tools ‐ wavelet coherency, wavelet phase and wavelet phase difference ‐ to assess the co‐movement between the Composite Leading Indicator and three macroeconomic variables ‐ Industrial Production Index, unemployment rate, and real GDP Growth ‐ at each frequency and moment. We also explored the lead–lag relationship between each pair of variables across time and frequency. We concluded that OECD's Composite Leading Indicator is a useful leading indicator of the Industrial Production Index and can be suited for forecasting the unemployment rate. However, it exhibited poor performance regarding GDP growth.
AIEgens for microorganism‐related visualization and therapy
Authors: Ye Ziyue , He Wei , Zhang Zicong , Qiu Zijie , Zhao Zheng , Tang Ben Zhong 
Published: January 2023

AbstractHumans and bacteria have always been closely related. However, pathogenic bacteria and drug‐resistant bacteria pose a certain threat to human life and health. On the other hand, probiotics, such as intestinal flora, also affect our daily life. Understanding the microstructure of microorganisms is important for effective treatment of bacterial infections. Luminogens with aggregation‐induced emission properties are now recognized as potent fluorescent agents for the diagnosis and treatment of microorganisms. In this review, we summarized the most recent developments of AIEgen‐based biomaterials and discussed the advantages of AIE fluorescent probes for imaging. Their applications for rapid imaging of bacteria, differentiation of Gram‐ negative and positive bacteria, and specific imaging of intracellular bacteria and fungi are presented. The monitoring of bacteria‐cell interaction was also introduced. Finally, the design strategy of engineered bacteria‐AIEgen hybrid system and their role in anti‐cancer applications was discussed.
Optimization and numerical simulation of the internal flow field of water–pesticide integrated microsprinklers
Authors: Liu Junping , Hussain Zawar , Wang Xinjian , Li Yangfan 
Published: January 2023

AbstractA new type of water–pesticide integrated microsprinkler has been developed in this study. Microsprinklers can irrigate crop roots to meet the irrigation index demand under low‐pressure conditions and apply pesticides on the backs of crop leaves to meet the spraying pesticide index demand under medium‐pressure conditions. In this paper, the structure and working principle of the water–pesticide integrated microsprinkler are discussed, and the key structural parameters are the number and diameter of the small round holes and the number and width of bevel grooves. Computational fluid dynamics (CFD) numerical simulations were performed to analyse the influence and variation in the key structural parameters on the microsprinkler flow field. The optimized structural parameters of the microsprinkler were determined: The outlet nozzle diameter was 3 mm, with three small round holes of 2 mm diameter and three bevel grooves of 0.8 mm width. The hydraulic performance of the optimized sprinkler and original sprinkler (outlet nozzle diameter was 2.5 mm, with two small round holes of 3 mm diameter and four bevel grooves of 1 mm width) were compared under different working pressure conditions. The results showed that the wetted radius and uniformity coefficient of the optimized sprinkler increased by 23% and 16%, respectively.
The quality of health data before and after the implementation of the electronic health management information system for the fistula program in Tanzania
Authors: Lwoga Edda Tandi , Musheiguza Edwin 
Published: January 2023

AbstractThis study assessed the quality and challenges of health data before and after the implementation of the Hospital Information System (HIS) for the fistula program at the Comprehensive Community‐based Rehabilitation Hospital in Tanzania. This was a mixed methods study. Data were collected at baseline and end‐line (before and after the implementation of the HIS) study using quantitative Data Quality Assessment (DQA) checklists and individual interviews. At the baseline, the DQA checklists were assessed using files registered from 2017 to May 2019. At the end‐line, the DQA checklists used data for a patient registered at the established HIS from June 2019 to December 2020. Interviews with clinical and non‐clinical staff were conducted at both baseline and end‐line studies. The study found that data quality improved after the implementation of the HIS. At baseline, data completeness declined from 85% in 2017 to 44.9% in 2019 while accuracy declined from 44% in 2017 to 31% in 2019. At the end‐line, data completeness and accuracy were >80% and 100% annually respectively. Data preciseness increased from 0% at baseline to 100% at the end‐line. Both data completeness and accuracy had inconsistent tend over months, although preciseness could not be evaluated at baseline as no breakdown of indicators was reported. The major challenges related to data quality included language barrier, incompleteness of data, incorrect data entry, use of both manual and electronic systems, as well as unreliable electricity and network. In general, the HIS had improved rates of data quality in terms of completeness, accuracy, and preciseness of data as compared to the manual system. Policy‐makers and hospital administrators should ensure that the HIS are integrated into national hospital information systems, build staff capacity, ensure reliable sources of electrical power, and regular training on data collection.
Using CBT interpersonal team formulation on mental health inpatient wards
Authors: Berry Katherine , Hartwell Rosalyn 
Published: January 2023

AbstractObjectivesTeam formulation involves a multidisciplinary team coming together to understand the factors leading to the development and maintenance of a patient's problems and the implications of this for future care planning.MethodThis paper presents a case example describing this approach in an acute inpatient setting.Results“William” was a 59‐year‐old male with longstanding mental health difficulties. During his admission, he presented as verbally abusive, intrusive, and sexually inappropriate. He was also controlling and intimidating toward other patients. Staff were becoming frustrated and hopeless about managing William on the ward leading them to adopt inconsistent approaches in relating to him. A team formulation was developed to understand William's trauma history and how this impacted on his beliefs about himself and other people as well as his way of relating to others.ConclusionsThe team formulation helped staff to develop consistent care plans in relation to William's presentation on the ward and more empathy in relation to behaviors that they found challenging.
Prospective comprehensive profiling of immune responses to COVID‐19 vaccination in patients on zanubrutinib therapy
Authors: Nguyen Thi H. O. , Lim Chhay , Lasica Masa , Whitechurch Ashley , Tennakoon Surekha , Saunders Natalie R. , Allen Lilith F. , Rowntree Louise C. , Chua Brendon Y. , Kedzierski Lukasz , Tan Hyon‐Xhi , Wheatley Adam K. , Kent Stephen J. , Karapanagiotidis Theo , Nicholson Suellen , Williamson Deborah A. , Slavin Monica A. , Tam Constantine S. , Kedzierska Katherine , Teh Benjamin W. 
Published: January 2023

AbstractZanubrutinib‐treated and treatment‐naïve patients with chronic lymphocytic leukaemia (CLL) or Waldenstrom's macroglobulinaemia were recruited in this prospective study to comprehensively profile humoral and cellular immune responses to COVID‐19 vaccination. Overall, 45 patients (median 72 years old) were recruited; the majority were male (71%), had CLL (76%) and were on zanubrutinib (78%). Seroconversion rates were 65% and 77% following two and three doses, respectively. CD4+ and CD8+ T‐cell response rates increased with third dose. In zanubrutinib‐treated patients, 86% developed either a humoral or cellular response. Patients on zanubrutinib developed substantial immune responses following two COVID‐19 vaccine doses, which further improved following a third dose.
The complex machinery of human cobalamin metabolism
Authors: McCorvie Thomas J. , Ferreira Douglas , Yue Wyatt W. , Froese D. Sean 
Published: January 2023

AbstractVitamin B12 (cobalamin, Cbl) is required as a cofactor by two human enzymes, 5‐methyltetrahydrofolate‐homocysteine methyltransferase (MTR) and methylmalonyl‐CoA mutase (MMUT). Within the body, a vast array of transporters, enzymes and chaperones are required for the generation and delivery of these cofactor forms. How they perform these functions is dictated by the structure and interactions of the proteins involved, the molecular bases of which are only now being elucidated. In this review, we highlight recent insights into human Cbl metabolism and address open questions in the field by employing a protein structure and interactome based perspective. We discuss how three very similar proteins—haptocorrin, intrinsic factor and transcobalamin—exploit slight structural differences and unique ligand receptor interactions to effect selective Cbl absorption and internalisation. We describe recent advances in the understanding of how endocytosed Cbl is transported across the lysosomal membrane and the implications of the recently solved ABCD4 structure. We detail how MMACHC and MMADHC cooperate to modify and target cytosolic Cbl to the client enzymes MTR and MMUT using ingenious modifications to an ancient nitroreductase fold, and how MTR and MMUT link with their accessory enzymes to sustainably harness the supernucleophilic potential of Cbl. Finally, we provide an outlook on how future studies may combine structural and interactome based approaches and incorporate knowledge of post‐translational modifications to bring further insights.