bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic striatal cholinergic interneuron excitation induces clinically-relevant dystonic behavior in mice
Authors: Gemperli Kat , Lu Xinguo , Chintalapati Keerthana , Rust Alyssa , Bajpai Rishabh , Suh Nathan , Blackburn Joanna , Gelineau-Morel Rose , Kruer Michael C. , Mingbundersuk Dararat , O’Malley Jennifer , Tochen Laura , Waugh Jeff , Wu Steve , Feyma Timothy , Perlmutter Joel , Mennerick Steven , McCall Jordan , Aravamuthan Bhooma R. 
Published: July 2023

AbstractDystonia is common, debilitating, often medically refractory, and difficult to diagnose. The gold standard for both clinical and mouse model dystonia evaluation is subjective assessment, ideally by expert consensus. However, this subjectivity makes translational quantification of clinically-relevant dystonia metrics across species nearly impossible. Many mouse models of genetic dystonias display abnormal striatal cholinergic interneuron excitation, but few display subjectively dystonic features. Therefore, whether striatal cholinergic interneuron pathology causes dystonia remains unknown. To address these critical limitations, we first demonstrate that objectively quantifiable leg adduction variability correlates with leg dystonia severity in people. We then show that chemogenetic excitation of striatal cholinergic interneurons in mice causes comparable leg adduction variability in mice. This clinically-relevant dystonic behavior in mice does not occur with acute excitation, but rather develops after 14 days of ongoing striatal cholinergic interneuron excitation. This requirement for prolonged excitation recapitulates the clinically observed phenomena of a delay between an inciting brain injury and subsequent dystonia manifestation and demonstrates a causative link between chronic striatal cholinergic interneuron excitation and clinically-relevant dystonic behavior in mice. Therefore, these results support targeting striatal ChIs for dystonia drug development and suggests early treatment in the window following injury but prior to dystonia onset.One Sentence SummaryChronic excitation of dorsal striatal cholinergic interneuron causes clinically-relevant dystonic phenotypes in mice
Slow-wave brain connectivity predicts executive functioning and group belonging in socially vulnerable individuals
Authors: Lanfranco Renzo C. , Santos Sousa Fabienne dos , Wessel Pierre Musa , Rivera-Rei Álvaro , Bekinschtein Tristán A. , Lucero Boris , Canales-Johnson Andrés , Huepe David 
Published: July 2023

ABSTRACTImportant efforts have been made to describe the neural and cognitive features of healthy and clinical populations. However, the neural and cognitive features of socially vulnerable individuals remain largely unexplored, despite their proneness to developing neurocognitive disorders. Socially vulnerable individuals can be characterised as socially deprived, having a low socioeconomic status, suffering from chronic social stress, and exhibiting poor social adaptation. While it is known that such individuals are likely to perform worse than their peers on executive function tasks, studies on healthy but socially vulnerable groups are lacking. In the current study, we explore whether neural power and connectivity signatures can characterise executive function performance in healthy but socially vulnerable individuals, shedding light on the impairing effects that chronic stress and social disadvantages have on cognition. We measured resting-state electroencephalography and executive functioning in 38 socially vulnerable participants and 38 matched control participants. Our findings indicate that while neural power was uninformative, lower delta and theta phase synchrony are associated with worse executive function performance in all participants, whereas delta phase synchrony is higher in the socially vulnerable group compared to the control group. Finally, we found that delta phase synchrony and years of schooling are the best predictors for belonging to the socially vulnerable group. Overall, these findings suggest that exposure to chronic stress due to socioeconomic factors and a lack of education are associated with changes in slow-wave neural connectivity and executive functioning.
Evidence for gene transfer between mycoviruses and their host:Curvulaviridaeas a case study
Authors: Maachi Ayoub , Alfonso Pau , Legarda Esmeralda G. , Wu Beilei , Elena Santiago F. 
Published: July 2023

AbstractGene transfer between distinct evolutionary lineages has been recognized as a frequent event occurring between viruses and their hosts. This phenomenon has been studied to some extent in animal and plant viruses, not so much in the case of mycoviruses, for which the evolutionary origins of their proteins remain poorly understood. In this study, we have tested the hypothesis of a mosaic origin for mycoviruses’ genomes, with the RNA-dependent RNA-polymerase (RdRp) being of viral origin and the coat protein (CP) resulting from one or more transfer events from the host genome. Firstly, phylogenetic trees were constructed for the RdRps and the CPs from a selection of viruses to address for possible incongruent evolutionary histories. Moreover, a PSI-BLAST search using the CP sequences from the different mycovirus groups retrieved hypothetical proteins (HP) with many orthologues in fungal genomes showing significant sequence homology with the CP from the members within theCurvulaviridaefamily. The structures of these HPs, predictedin silicousing AlphaFold, tend to show high similarity with viral CPs suggesting the occurrence of gene transfer between viruses and fungi, although no clear function has been yet attributed to these genes in the host. Phylogenetic analyses suggest that this gene transfer could have occurred in multiple independent events. Additional selection analysis supports the notion that the most parsimonious explanation is the transfer of the HP from the host to an ancestral viral genome followed by fast evolution to accommodate the newly acquired protein to function as a CP.
Mechanism of outer kinetochore assembly on microtubules and its regulation by mitotic error correction
Authors: Muir Kyle W. , Batters Christopher , Dendooven Tom , Yang Jing , Zhang Ziguo , Burt Alister , Barford David 
Published: July 2023

AbstractKinetochores couple chromosomes to the mitotic spindle and transduce the energy of microtubule depolymerization to segregate the genome during cell division. Kinetochore – microtubule attachments are often initially erroneous and subject to a mitotic error correction (EC) mechanism that drives their turnover until biorientation is achieved. How this is accomplished and regulated, and how kinetochore-mediated chromosome segregation occurs at a molecular level remain major outstanding questions. Here we describe the cryo-electron microscopy (cryo-EM) structure of the budding yeast outer kinetochore Ndc80 and Dam1 ring complexes assembled onto microtubules. We observe coordinated interactions of the outer kinetochore complexes through multiple interfaces, in addition to a short staple within the Dam1 subunit that facilitates Dam1c ring assembly. Perturbation of these interfaces results in loss of yeast viability. Force-rupture assays indicated this is a consequence of substantial reductions in kinetochore – microtubule binding strength. EC-mediated phosphorylation of Ndc80c-Dam1c interfaces would drive complex disassembly, whereas Dam1 staple phosphorylation would promote Dam1c ring disassembly, explaining how kinetochore – microtubule attachments are destabilized and reset by the EC mechanism.One-Sentence SummaryPhosphorylation disrupts the outer kinetochore to regulate kinetochore-microtubule attachments in mitotic error correction.
Characterization of RBM15 protein binding with long noncoding RNAs
Authors: Bose Emma , Mayes Caleb , Ellis Lance , Baker Corrine , Tambalotti Sofia , Xiong Shengwei , Osei Sarpong Yaa Pokua , Shalaby Marwan , Barry Lucas , Lewis Frank , Joseph Johnson , Isaacs Talaidh , McCarthy Derik , Katz Dana , Wang Jingyang , Zirimu Victoria , Vargas Luis , Von Hofe Julian , Aguilar Glen , Buchan Katherine , Zheng Lei , Wolfe Gregory , Jones Alisha N 
Published: July 2023

AbstractThe RNA binding motif 15 protein (RBM15) plays a critical role in post-transcriptional regulation. Its role in facilitating m6A modification, specifically through guiding the writer complex (WTAP METTL13 METTL14) to DRACH sequence motifs, has been demonstrated for several RNAs, including long noncoding RNAs (lncRNAs). The structural mechanism that underlies how RBM15 interacts with RNA has yet to be elucidated. In this study, we mined and bioinformatically assessed publicly available genome wide RNA 2D structural probing and RBP cross linking and immunoprecipitation data to investigate the propensity of lncRNAs to interact with RBM15. We then experimentally characterized how this interaction occurs with two lncRNAs, FIRRE and XIST. RBM15, which possesses three RNA recognition motifs (RRMs), primarily interacts with stem loop structures adopted by lncRNAs through its two terminal RRMs, RRMs 2 and 3. Using solution NMR, we find RRMs 2 and 3 are rigidly confined in solution, in the absence of RNA. Altogether, this work provides clarity on the molecular mechanism by which RBM15 interacts with RNAs to govern biological function.
Transformational leadership, job engagement, and championing behavior: assessing the mediating role of role clarity
Authors: Majid Fatima , Raziq Muhammad Mustafa , Memon Mumtaz Ali , Tariq Adeel , Rice John Lewis 
Published: July 2023

PurposeThis paper aims to examine how role clarity mediates the effect of transformational leadership on job engagement and championing behavior in support of the conservation of resources theory.Design/methodology/approachThe authors used a cross-sectional approach to collect data from managerial/nonmanagerial employees within the Pakistani hospitality industry via online and offline questionnaires. A total of 170 responses were used in the data analysis using partial least squares structural equation modeling to test the hypothesized relationships.FindingsFindings show that transformational leadership directly predicts improved role clarity and job engagement. Moreover, role clarity leads to job engagement and championing behavior. Role clarity exhibits a partial mediation effect on job engagement and full mediation on championing behavior.Originality/valueTo bridge the gap in leadership literature, this research assesses the underlying effect of role clarity on the relationship between transformational leadership and its positive outcomes. It provides theoretical and managerial implications regarding the role of transformational leadership characteristics and outcomes.
Augmented reality-based facility maintenance management system
Authors: Fan Su-Ling , Ong Wei-San , Wu Chun-Tin , Forcada Matheu Nuria , Alavi Hamidreza 
Published: July 2023

Purpose
The purpose of this paper is to address the problems of the current facilities maintenance management (FMM) system in finding necessary information, identifying defective facilities and prioritizing maintenance work orders.

Design/methodology/approach
In this paper, in conjunction with building information modeling, a system is proposed to perform a preliminary inspection of each maintenance request, provide FMM staff with the location of the faulty facility and its associated details and provide recommendations for prioritizing repair work orders. Unity and Revit are used to implement the proposed system and a case study is conducted to demonstrate its effectiveness.

Findings
An augmented reality (AR)-FMM system was developed using the AR technique in this paper. This system provides the related information even if the FMM receives a problem report without facility information from the occupant and performs a preliminary inspection so that the faulty facility and the route to it are identified. In addition, a work order sequence of pending requests was provided. The visualization of the facility using AR technology has brought great convenience and ease to FMM staff.

Originality/value
This paper addresses the problems encountered in the current facility maintenance management system concerning AR technology.
Pro-LGBTQIAP+ counterintuitive communication toward the consumer
Authors: Andreoli Taís Pasquotto , Assis Vieira Bárbara 
Published: July 2023

PurposeThe study aims to analyze consumer reaction to counterintuitive communication linked to the LGBTQIAP+ cause.Design/methodology/approachThe theoretical framework focused on marketing communication, counterintuitive communication and the insertion of the LGBTQIAP+ issue in them. A hypothetical-deductive approach was adopted, carried out through an online experiment with a factorial design 2 (high involvement product − wedding ring × low involvement − perfume) × 2 (homosexual couple − counterintuitive × heterosexual − intuitive), with two subsequent steps: eye tracker collection (n = 21) and questionnaire application (n = 136).FindingsIt was possible to attest to the differences between traditional (intuitive) and counterintuitive marketing communications, identified both in terms of visualizations and fixations (eye tracking), and objective responses (online questionnaire), finding more positive consumer behavior in the case of counterintuitive communication with insertion of the LGBTQIAP+ cause.Research limitations/implicationsMarketing communication has great potential to create “new/other” values and worldviews, thus having an important role in social responsibility that goes beyond the marketing sphere. Precisely in this context, the study contributes to endorse the literature concerning counterintuitive communication, reinforcing the increasing importance and favorable scenario for its practice, as well as highlighting its importance as an efficient marketing strategy.Originality/valueThe study extends the understanding of counterintuitive communication with the scope of an investigation linked to the LGBTQIAP+ cause, especially from the perspective of the receiving public, the consumers. Furthermore, it advances in the sense of aggregating empirical evidence by means of both exploratory (with neuroscientific technique as eye tracking) and explanatory factors.
Training as a facilitator for Industry 4.0
Authors: Di Sabato Vito , Savov Radovan 
Published: July 2023

Purpose This paper studies the impact of certain characteristics of companies to training programs in the Industry 4.0 (I4.0) context. Partial objective is to rank the main human barriers companies have to overcome so that they can digitalize.Design/methodology/approach To accomplish the objectives, a closed-ended questionnaire was sent to Slovak and Italian companies and analyzed using statistical nonparametric tests. The partial objective was achieved using the so-called Henry-Garrett’s ranking method.Findings Results show the significance impact of companies’ characteristics such as foreign participation and company dimension on training practices whereas economic situation (financial health) seems not to influence it.Research limitations/implications The study may lack generalizability as only 102 answers were collected. Perhaps, the outcome would be different with another sample from other countries. Moreover, using closed-ended questions, certain features may not have been covered.Practical implications Companies should always guarantee training for the resulted benefits. It is fundamental for organizations to find a time gap, resources and professionals who can teach these programs. Even when companies are incurring financial problems they should do so since human capital development can increase their competitiveness. The most critical barriers should be carefully addressed by companies. Training can help to overcome I4.0 barriers related to Human Resources (HR) and contribute to its growth.Originality/value This paper gives insights of the impact of certain characteristics of companies to the training programs. Because past research has limited their analysis on the identification of barrier, its novelty lies in the attempt to rank the most significant barriers among those detected by other authors in previous research.
The impact of a new women's professional sport league on pre-existing sport organisations: a modified, integrative approach to examining change
Authors: Taylor Chelsey Sara , Naraine Michael L. , Rowe Katie , Robertson Jonathan , Karg Adam 
Published: July 2023

PurposeThe purpose of this study was to explore the process of change in existing professional sport organisations as they initiate a women's team.Design/methodology/approachThree Australian Football League clubs with licenses for professional women's teams were examined, with semi-structured interviews held with three key department managers from each club.FindingsThe findings suggest organisations adopt either a community-focused or commercially focused approach, the selection of which is a response to the interplay of institutional pressures (e.g. league demands), resource demands (e.g. human and financial) and the strategic choices of a few, key “idea champions”.Originality/valueThis study provides insight into the approach change taken by clubs as they introduce a women's team into their existing organisational structure.
Motivational catalysts: the dominant role between prosocial personality and social entrepreneurial intentions among university students
Authors: Cheah Jeffrey S.S. , Loh ShihYee , Gunasekaran Angappa 
Published: July 2023

Purpose
Social entrepreneurship has the potential to address societal challenges, and high-education students are expected to be future social leaders. However, engagement in social entrepreneurship remains low in many countries. This study aims to investigate the mediating role of motivational mechanisms (i.e. self-efficacy, social support and social worth) in the relationship between prosocial personality and social entrepreneurial intentions (SEIs).

Design/methodology/approach
Based on the theory of planned behaviour, the authors conducted a survey of 292 valid respondents from 35 major public and private universities. The collected data were analysed using the structural equation modelling technique. The reliability of the measurements and the model’s predictive capabilities are substantial and assured.

Findings
The findings suggest that prosocial personality alone does not directly predict social entrepreneurial behaviour. However, motivational forces are dominant mediators in the relationship between prosocial personality and SEIs. Specifically, self-efficacy, social support and social worth significantly mediate this relationship.

Research limitations/implications
The findings of this study provide insights into why prior studies on this topic has produced contradictory results.

Practical implications
The authors recommend that universities and policymakers provide adequate cognitive learning experiences, capacity-building programmes, funding support and recognition to enhance graduates’ inner strength and foster SEIs.

Originality/value
The empirical results resolve the contradictions found in many prior studies and highlight the importance of supportive mechanisms when promoting SEI in emerging regions.
Attracting Gen Z workforce: the effects of organizational social media policy
Authors: Zhong YunYing , Zhang Lu , Wei Wei , Chang Jerry Cha-Jan 
Published: July 2023

Purpose
Considering Gen Z’s unique formative experience with social media, this study aims to apply the cue utilization theory to investigate the effects of social media policy as an organizational cue in attracting this cohort to hospitality and tourism (H&T) companies.

Design/methodology/approach
A 2 (policy frame: promotion/prevention) × 2 (policy boundary: distinct/permeable) experiment was conducted with 243 Gen Z H&T job seekers. ANCOVA analyses were performed to analyze the effects of policy frame and boundary on perceived organization attractiveness and pursuit intention, respectively.

Findings
This study found that when a company policy promoted social media usage, Gen Z job seekers reported higher attractiveness and pursuit intention in the distinct boundary condition. However, when a company’s social media policy emphasized a prevention frame, Gen Z job seekers reported a similar level of perceived attractiveness and pursuit intention, regardless of the policy boundary conditions.

Originality/value
This study addressed the literature gaps of limited research on the Gen Z workforce and the effects of social media policy on talent attraction. Based on the cue utilization theory, this study identified the policy clause combinations that generated positive recruitment impacts among Gen Z members. The study provided unique theoretical and practical implications for H&T managers to use social media policy as a novel and cost-effective tool to attract Gen Z talents.
Sodium alginate coating of Ginkgo biloba leaves extract containing phenylpropanoids as an ecofriendly preserving agent to maintain the quality of peach fruit
Authors: Mou Linyun , Lu Ya , Zhang Jin , Bilal Muhammad , Li Jianlong , Li Ganpeng 
Published: July 2023

AbstractPlant constituents are of great interest in the food processing industry as potential natural preservative agents for controlling foodborne pathogens. In this study, the 95% EtOH/H2O extract of Ginkgo biloba leaves was separated using polarity extraction solvents with petroleum ether (PE), ethyl acetate (EA), n‐butanol (nB), and water (W) by the principle of similarity and compatibility. Through TLC and NMR analysis of these extracts, it can be concluded that the main component of PE extract were organic acids, for EA extract were flavonoids, for nB extract were phenylpropanoids, and water extract were oligosaccharides. Twelve monomer compounds were separated from the extracts to verify the composition of each extraction stage. Results of morphological and molecular identification revealed that Monilinia fructicola and Rhizopus stolonifer were the main fungi causing peach rot. After evaluating the antifungal activity and peach quality of the four extract/sodium alginate coatings, it was found that the n‐butanol extract/sodium alginate coating containing phenylpropanoids had the lowest decay index and the best preservation effect, providing a sustainable alternative to reduce the harm to the environment of synthetic preservatives.Practical ApplicationThe abuse of synthetic preservatives poses a threat to the ecological environment and physical health. Therefore, this study developed sodium alginate coating of Ginkgo biloba leaves extract containing phenylpropanoids, which has good effects on the preservation of peaches. The agent is a promising environmentally friendly alternative for synthetic preservatives.