bluuebunny/crossref_metadata_2025_split huggingface dataset:
XIVModern Literature
Authors: Sorensen Jennifer , Mourant Chris , Phillips Joshua , Parsons Katherine , Roach Rebecca , Stringfellow Sophie , Rodríguez Martín Gustavo A , Fallow Catriona , Richards Joshua , Niven Alex , Wheatley David 
Published: August 2023

AbstractThis chapter has eight sections 1. General. 2. Fiction Pre-1945; 3. Fiction Post-1945; 4. Drama Pre-1950; 5. Drama Post-1950; 6. British Poetry Pre-1950; 7. British Poetry Post-1950; 8. Modern Irish Poetry. Section 1 is by Jennifer Sorensen; section 2(a) is by Chris Mourant; section 2(b) will resume in 2023; section 2(c) is delayed by parental leave and will resume in 2023; section 2(d) is by Joshua Phillips; section 3(a) is by Katherine Parsons; section 3(b) is by Rebecca Roach, with thanks to Samuel Cooper, and covers work in 2020 and 2021; section 4(a) is by Sophie Stringfellow; section 4(b) is by Gustavo A. Rodríguez Martín; section 5 is by Catriona Fallow; section 6(a) will resume in 2023; section 6(b) is by Joshua Richards; section 7 is by Alex Niven and covers work in 2020 and 2021; section 8 is by David Wheatley.
Sex-related exacerbation of injury-induced mechanical hypersensitivity in GAD67 haplodeficient mice
Authors: Charron Aude , Pepino Lucie , Malapert Pascale , Debrauwer Vincent , Castets Francis , Salio Chiara , Moqrich Aziz 
Published: August 2023

Abstract
Decreased GABA levels in injury-induced loss of spinal inhibition are still under intense interest and debate. Here, we show that GAD67 haplodeficient mice exhibited a prolonged injury-induced mechanical hypersensitivity in postoperative, inflammatory, and neuropathic pain models. In line with this, we found that loss of 1 copy of the GAD67-encoding gene Gad1 causes a significant decrease in GABA contents in spinal GABAergic neuronal profiles. Consequently, GAD67 haplodeficient males and females were unresponsive to the analgesic effect of diazepam. Remarkably, all these phenotypes were more pronounced in GAD67 haplodeficient females. These mice had significantly much lower amount of spinal GABA content, exhibited an exacerbated pain phenotype during the second phase of the formalin test, developed a longer lasting mechanical hypersensitivity in the chronic constriction injury of the sciatic nerve model, and were unresponsive to the pain relief effect of the GABA-transaminase inhibitor phenylethylidenehydrazine. Our study provides strong evidence for a role of GABA levels in the modulation of injury-induced mechanical pain and suggests a potential role of the GABAergic system in the prevalence of some painful diseases among females.
How do opioids control pain circuits in the brainstem during opioid-induced disorders and in chronic pain? Implications for the treatment of chronic pain
Authors: Costa Ana Rita , Tavares Isaura , Martins Isabel 
Published: August 2023

Abstract
Brainstem areas involved in descending pain modulation are crucial for the analgesic actions of opioids. However, the role of opioids in these areas during tolerance, opioid-induced hyperalgesia (OIH), and in chronic pain settings remains underappreciated. We conducted a revision of the recent studies performed in the main brainstem areas devoted to descending pain modulation with a special focus on the medullary dorsal reticular nucleus (DRt), as a distinctive pain facilitatory area and a key player in the diffuse noxious inhibitory control paradigm. We show that maladaptive processes within the signaling of the µ-opioid receptor (MOR), which entail desensitization and a switch to excitatory signaling, occur in the brainstem, contributing to tolerance and OIH. In the context of chronic pain, the alterations found are complex and depend on the area and model of chronic pain. For example, the downregulation of MOR and δ-opioid receptor (DOR) in some areas, including the DRt, during neuropathic pain likely contributes to the inefficacy of opioids. However, the upregulation of MOR and DOR, at the rostral ventromedial medulla, in inflammatory pain models, suggests therapeutic avenues to explore. Mechanistically, the rationale for the diversity and complexity of alterations in the brainstem is likely provided by the alternative splicing of opioid receptors and the heteromerization of MOR. In conclusion, this review emphasizes how important it is to consider the effects of opioids at these circuits when using opioids for the treatment of chronic pain and for the development of safer and effective opioids.
Impact of immunosuppression level on liver allograft fibrosis after pediatric liver transplantation: a retrospective cohort study
Authors: Jiang Yi-Zhou , Zhao Xin-Yan , Zhou Guang-Peng , Wei Lin , Qu Wei , Zeng Zhi-Gui , Wu Shan-Shan , Zhang Hai-Ming , Liu Ying , Tan Yu-Le , Wang Jun , Zhu Zhi-Jun , Sun Li-Ying 
Published: August 2023

Background:
Liver allograft fibrosis (LAF) is prevalent among children with long-term survival after liver transplantation (LT). We aimed to identify clinical risk factors, with a focus on the impact of immunosuppression (IS) level in the early posttransplant period on LAF.

Methods:
A retrospective study was conducted on pediatric LT recipients with at least one year of follow-up. Cox regression models were used to analyze risk factors associated with LAF, and landmark analysis was used to evaluate the impact of IS level on LAF. Longitudinal analysis was also conducted in patients with paired biopsies.

Results:
A total of 139 patients involving 174 liver biopsies were included. With 2.3 to 5.9 years of follow-up, LAF was detected in 91.4% of patients (7.9% were significant), up to 88.2% of whom showed normal liver function. Episodes of acute rejection, biliary complications, CMV infection and prolonged cold ischemia time were independent risk factors. Besides, the risk of LAF in patients with relatively low IS levels at postoperative 1-3, 3-6, 6-12, and 12-36 months was higher than the counterparts. Especially, in patients with relatively high IS levels (mean tacrolimus trough concentration ≥5.1 ng/mL) during postoperative 12-36 months, the risk of LAF was 67% lower in the short future (P=0.006). In paired analysis, patients with increased IS levels were more likely to achieve fibrosis reduction (HR=7.53, P=0.025).

Conclusions:
Mild to moderate LAF is common among pediatric LT recipients and can appear early and silently. Maintaining adequate levels of immunosuppression during 1 to 3 years after LT seems crucial to ensure protection against LAF.
The role of aspirin versus low-molecular-weight heparin for venous thromboembolism prophylaxis after total knee arthroplasty: a meta-analysis of randomized controlled trials
Authors: Meng Jiahao , Liu Weijie , Xiao Yifan , Tang Hang , Wu Yumei , Gao Shuguang 
Published: August 2023

Background:
There is a debate over the use of aspirin after total knee arthroplasty (TKA) for venous thromboembolism (VTE) prophylaxis. To evaluate the clinical effectiveness and safety of aspirin after TKA, we performed a meta-analysis of randomized controlled trials (RCTs) that compared aspirin with low-molecular-weight-heparin (LMWH).

Methods:
PubMed, Cochrane Library, Embase, and Web of Science were last searched on 31 January 2023 for studies comparing the effect of venous thromboembolism prophylaxis between aspirin and LMWH. The results of eligible studies were analyzed in terms of venous thromboembolism, deep venous thrombosis (DVT), pulmonary embolism (PE), and bleeding complications rates.

Results:
Six randomized controlled trials including 6772 patients met the inclusion criteria. LMWH showed a statistically significant reduction in the overall VTE rate (RR 1.46, 95%CI 1.16 to 1.84). No significant differences in DVT, PE, and bleeding complications rates were found between aspirin and LMWH.

Conclusion:
Compared with LMWH, aspirin presents a higher risk of VTE after TKA. In terms of safety, aspirin and LMWH show comparable outcomes. The results do not support the role of aspirin role as an anticoagulant for preventing VTE after TKA.
Performance of CT-based deep learning in diagnostic assessment of suspicious lateral lymph nodes in papillary thyroid cancer: a prospective diagnostic study
Authors: Zheng Guibin , Zhang Haicheng , Lin Fusheng , Zafereo Mark , Gross Neil , Sun Peng , Liu Yang , Sun Haiqing , WU Guochang , Wei Shujian , Wu Jia , Mao Ning , Li Guojun , Wu Guoyang , Zheng Haitao , Song Xicheng 
Published: August 2023

Background:
Preoperative evaluation of the metastasis status of lateral lymph nodes (LNs) in papillary thyroid cancer (PTC) is challenging. Strategies for using deep learning (DL) to diagnosis of lateral LN metastasis require additional development and testing. This study aimed to build a DL-based model to distinguish benign lateral LNs from metastatic lateral LNs in PTC and test the model’s diagnostic performance in a real-world clinical setting.

Methods:
This was a prospective diagnostic study. An ensemble model integrating a three-dimensional residual network (ResNet) algorithm with clinical risk factors available before surgery was developed based on CT images of lateral LNs in an internal dataset and validated in two external datasets. The diagnostic performance of the ensemble model was tested and compared with the results of fine-needle aspiration (FNA) (used as the standard reference method) and the diagnoses made by two senior radiologists in 113 suspicious lateral LNs in patients enrolled prospectively.

Results:
The area under the receiver operating characteristic curve of the ensemble model for diagnosing suspicious lateral LNs was 0.824 (95% CI, 0.738-0.911). The sensitivity and specificity of the ensemble model were 0.839 (95% CI, 0.762-0.916) and 0.769 (95% CI, 0.607-0.931), respectively. The diagnostic accuracy of the ensemble model was 82.3%. With FNA results as the criterion standard, the ensemble model had excellent diagnostic performance (P=0.115), similar to that of the two senior radiologists (P=1.000 and P=0.392, respectively).

Conclusion:
A three-dimensional ResNet-based ensemble model was successfully developed for diagnostic assessment of suspicious lateral LNs and achieved diagnostic performance similar to that of FNA and senior radiologists. The model appears promising for clinical application.
Vibration Controlled Transient Elastography in Screening for Silent Liver Diseases
Authors: Said Ebada , Abdel-Gawad Muhammad , Abdelsameea Eman , Elshemy Eman Elsayed , Abdeen Nermeen , Elbasiony Mohamed , Omran Dalia , Elesnawy Yasser , Eid Alshaimaa , Lashen Sameh A. , Elbahr Osama , Naguib Gina Gamal , Afify Shimaa , Bahbah Eshak I. , Alboraie Mohamed 
Published: August 2023

Objectives
The objective of this study was to screen for significant hepatic fibrosis or steatosis in asymptomatic, apparently healthy subjects by using Vibration-controlled transient elastography and controlled attenuation parameter (CAP).

Methods
Prospectively, 433 asymptomatic apparently healthy adults were included. Fibroscan/CAP examination was performed for all of them. Subjects with liver stiffness measurement > 6 kPa or CAP >248 dB/m were further evaluated to assess underlying chronic liver disease.

Results
According to fibroscan/CAP examination, subjects were classified into four subgroups: normal (119) with CAP score of 215.85 ± 24.81 dB/m and fibrosis score of 4.47 ± 0.81 kPa, subjects with steatosis only 133 with CAP score of 309.41 ± 42.6 dB/m and fibrosis score of 4.74 ± 0.82 kPa, subjects with both steatosis and fibrosis 95 with CAP score of 318.20 ± 39.89 dB/m and fibrosis score of 7.92 ± 2.58 kPaand subjects with fibrosis only 86 with CAP score of 213.48 ± 22.62 dB/m and fibrosis score of 6.96 ± 1.11 kPa. S0 was present in 205 (47.3%), S1 in 48 (10.2%), S2 in 16 (3.7%) and S3 in 168 (38.8%) of studied subjects, whereas F0-1 was present in 371 (85.7%), F2 in 44 (10.16%), F3 in 16 (3.7%) subjects and F4 in only one (0.23%) subject. Subjects with both steatosis and fibrosis showed significantly higher transaminases, triglycerides and total cholesterol levels than other subgroups.

Conclusions
Most asymptomatic, apparently healthy subjects (72%) have significant steatosis and fibrosis. Liver stiffness measurement and CAP might represent promising first-line noninvasive procedures to screen for silent liver diseases in the general population.
Exploratory analysis of 64CuCl2 PET-CT imaging in carcinoma prostate and its comparison with 68Ga-PSMA-11 and 18F-FDG PET-CT
Authors: Nazar Aamir K , Kalshetty Ashwini , Chakravarty Rubel , Chakraborty Sudipta , Basu Sandip 
Published: August 2023

Aim
Exploratory analysis of 64CuCl2 PET-CT imaging in patients of carcinoma prostate and its head-to-head comparison with 68Ga-PSMA-11 and 18F-FDG PET-CT.

Methods
In this prospective study, 50 patients of biopsy-proven carcinoma prostate belonging to the entire spectrum of disease were evaluated, out of which 21 patients were for initial staging and 29 were for restaging/response evaluation. Both 64CuCl2 (early and delayed) and 68Ga-PSMA-11 PET-CT were undertaken in all patients and 18F-FDG PET-CT was done in patients whenever possible. All scans were done within a period of 2 weeks, without any interim therapeutic intervention. 64CuCl2 PET-CT was acquired at 1 and 3 h. We evaluated the physiological uptake of 64CuCl2, correlated the uptake in primary with disease parameters like Gleason score and serum PSA levels, and compared the detection rates for primary and metastatic disease with 68Ga-PSMA-11 and 18F-FDG PET-CT.

Results
The detection rates of primary disease were same for both 64CuCl2 and 68Ga-PSMA-11 PET-CT and both agents performed similarly in detecting extra-prostatic disease. There was no statistically significant correlation observed between the uptake of 64CuCl2 in the primary lesion with disease parameters. With regard to the evaluation of metastatic disease, the detection rate of 64CuCl2 PET-CT was 86% for lymph nodes, 77.3% for skeletal metastases and 80.6% for soft tissue metastases while 68Ga-PSMA-11 PET-CT performed better with detection rates were 98%, 99% and 85.4%, respectively. In 17 patients where 18F-FDG PET-CT was available, 64CuCl2 PET-CT detected more metastatic disease than 18F-FDG PET-CT.

Conclusion

64CuCl2 PET-CT did not show any additional advantage over 68Ga-PSMA-11 PET-CT in evaluation of local disease or for the assessment of metastatic disease. When compared to 68Ga-PSMA-11 PET-CT, the absence of urinary bladder and ureteric activity allows better contrast for evaluating local disease, but it does not translate into increased disease detection.
Sinisalibacter aestuarii sp. nov., isolated from estuarine sediment of the Arakawa River
Authors: Arafat Shaikh Tareq , Hirano Shiori , Sato Anju , Takeuchi Katsunori , Yasuda Tetsuya , Terahara Takeshi , Hamada Moriyuki , Kobayashi Takeshi 
Published: August 2023

A Gram-stain-negative, rod-shaped, non-motile and strictly aerobic bacterium, which showed biofilm-forming ability on polystyrene, designated as strain B-399T, was isolated from the estuarine sediment of the Arakawa River near Tokyo Bay. It grew at pH 6.0–8.5, at 15–35 °C and in the presence of 0–7.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B-399T was clustered in the genus

Sinisalibacter

and has 96.94 % sequence similarity to

Sinisalibacter lacisalsi

X12M-4T, which was the only validly described species in this genus. On the basis of our genome sequencing analyses, the average nucleotide identity and digital DNA–DNA hybridization values between strains B-399T and

S. lacisalsi

X12M-4T were 79.54 and 22.30 %, respectively, which confirms that strain B-399T represents a novel species of the genus

Sinisalibacter

. The draft genome size and the DNA G+C content of strain B-399T were 4.12 Mb and 65.2 mol%, respectively. The major fatty acids (>10 %) of strain B-399T were C16 : 0, summed feature 8 (C18 : 1
ω6c and/or C18 : 1
ω7c) and C19 : 0 cyclo ω8c. The polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and unidentified lipids. The respiratory quinone was Q-10. These chemotaxonomic features were almost coincident with those of the genus

Sinisalibacter

. Therefore, strain B-399T should be classified as representing a new species of the genus

Sinisalibacter

, for which the name Sinisalibacter aestuarii sp. nov. is proposed. The type strain is B-399T (=NBRC 115629T=DSM 114148T).
Phage-plasmids promote genetic exchanges between phages and plasmids and create novel ones
Authors: Pfeifer Eugen , Rocha Eduardo P.C. 
Published: August 2023

AbstractPhages and plasmids have key roles in bacterial evolution and are usually very different. Yet, they must recombine, since they sometimes carry nearly identical accessory genes. Phage-plasmids are both plasmids and phages and we found they connect them by being at their interface in the network of gene repertoire relatedness. We searched for recombining genes in all three types of elements and found that phage-plasmid exchange rates are between those of plasmids (highest) and phages (lowest). Phage-plasmids drive gene flow between phages and plasmids, including that of antibiotic resistances and defense systems. Genetic exchanges and gene inactivation resulted in P1-like phage-plasmids turning into integrative prophages or just plasmids. The latter acquired conjugation-related functions thereby transitioning from viral to conjugation-driven horizontal transfer. Phage-plasmids have thus a key role in spreading traits across mobile genetic elements and their hosts, while facilitating conversions of one type of element into another.
A novel architecture of PT neuron-based corticothalamic connectivity in the auditory system
Authors: Xie Fenghua , Gao Yixiao , Wang Tao , Liu Mengting , Yuan Kexin 
Published: August 2023

AbstractLargely topographical projections from different modules of the thalamus, such as the primary, secondary and association sensory thalamus, to hierarchically defined cortical areas have been recognized across sensory systems. However, how corticothalamic projections, which are believed to be crucial for the remarkable flexibility and precision exhibited by our sensory systems, are organized remained poorly understood compared with the thalamocortical counterpart. Here we report that, first, the primary auditory thalamus received direct inputs from cortical L5 neurons. Second, in contrast to the robust thalamocortical topography, L5 neurons in each of the primary, secondary and association auditory cortical regions project to each individual module of the auditory thalamus at the macroscale. Third, the association cortex provided the most L5 inputs to all thalamic modules followed by the secondary and primary auditory cortices. Lastly, L5 axon terminals were mainly varicosity-type and evenly distributed across thalamic modules, but those in the polymodal association module were the largest. Our data suggest that all the modules of the auditory thalamus may be under the modulation of common L5 inputs. This fully-connected-like corticothalamic architecture urges a revision of the traditional hierarchical model in the sensory systems.
Efficient assembly of plant genomes: A case study with evolutionary implications inRanunculus(Ranunculaceae)
Authors: Karbstein Kevin , Choudhary Nancy , Xie Ting , Tomasello Salvatore , Wagner Natascha D. , Barke Birthe H. , Paetzold Claudia , Bradican John P. , Preick Michaela , Himmelbach Axel , Stein Nils , Papantonis Argyris , Irisarri Iker , de Vries Jan , Pucker Boas , Hörandl Elvira 
Published: August 2023

AbstractCurrently, it is still a challenge - in terms of laboratory effort and cost, as well as assembly quality - to unravel the sequence of large and complex genomes from non-model/crop plants. This often hampers the study of evolutionarily intricate species groups. The species-rich genusRanunculus(Ranunculaceae) is an important angiosperm group for the study of polyploidy, apomixis, reticulate evolution, and biogeography. However, neither mitochondrial, nor high-quality nuclear genome sequences are available. This limits phylogenomic, functional, and taxonomic analyses thus far. Here, we tested Illumina short- read, Oxford Nanopore Technology (ONT) or PacBio/HiFi long-read, and hybrid-read assembly strategies. We used the diploid progenitor speciesR. cassubicifolius(R. auricomuscomplex), and selected the best assemblies in terms of completeness, contiguity, and quality scores. We first assembled the plastome (156 kbp, 85 genes) and mitogenome (1.18 Mbp, 40 genes) sequences using Illumina and Illumina-PacBio-hybrid strategies, respectively. We also present an updated plastome and the first mitogenome phylogeny of Ranunculaceae, including studies of gene loss (e.g.,infA,ycf15, orrps) with evolutionary implications. For the nuclear genome, we favored a PacBio-based assembly three-times polished with filtered reads and subsequently scaffolded into 8 pseudochromosomes by chromatin conformation data (Hi-C) as the representative sequence. We obtained a haploid genome sequence with 2.69 Gbp, 94.1% complete BUSCO ‘embryophyta_odb10’ genes found, and 31,322 annotated genes. The genomic information presented here will improve phylogenomic analyses in this species complex, and will enable advanced functional, evolutionary, and biogeographic analyses for the genus and beyond Ranunculaceae in the future.Significance StatementThe genusRanunculusis a model system in flowering plants for polyploidy, apomixis, evolution, and biogeography research. We present the first nuclear and mitochondrial genome sequence, and the plastid evolution of Ranunculaceae. Using Illumina, ONT, and PacBio data, we developed an efficient assembly strategy that can be applied to other non-model plants. Results presented here are useful for improving population genomic and phylogenomic analyses, and enable better functional analyses at species, genus and family level.
Neolactotetraosylceramide: A novel non-invasive urinary biomarker for bladder cancer
Authors: Moreira Inês B. , Rossdam Charlotte , Beimdiek Julia , Vicente Manuel M. , Schmitz Jessica , Oberbeck Astrid , Bräsen Jan H. , Tezval Hossein , Buettner Falk F. R. 
Published: August 2023

ABSTRACTThere is an urgent need to identify noninvasive biomarkers for bladder cancer. Here, we applied glycan analytics by multiplex capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF) to unravel the global glycosphingolipid (GSL)-glycan profile of primary tumor tissues and urine samples from bladder cancer patients. Thereby, we detected neolactotetraosylceramide (Galβ1-4GlcNAcβ1-3Galβ1-4Glc-Cer, nLc4) at significantly increased levels from tumorigenic regions of bladder tissues compared to non-malignant adjacent material (n = 30). Specific expression of nLc4 in cancer tissue was confirmed by immunofluorescence staining. GSL-glycan profiling by xCGE-LIF of urinary exosomes showed that nLc4 is increased in bladder cancer patients (n = 16) when compared to controls (n = 50), with an overall sensitivity of 57% and specificity of 90%. We set-up an ELISA targeting nLc4-containing urinary exosomes from bladder cancer patients (n = 9) and cancer-free individuals (n = 9) demonstrating an overall sensitivity and specificity of 89% and 78%, respectively.SIGNFICANCEThis study shows that levels of nLc4 are significantly elevated in bladder cancer tissue and urinary exosomes of bladder cancer patients. Urinary detection of nLc4 by glycan analytics or ELISA outperforms standard diagnostic modalities, facilitating noninvasive bladder cancer diagnosis. Furthermore, nLc4 bears the potential of being a target for bladder cancer therapy.