bluuebunny/crossref_metadata_2025_split huggingface dataset:
Geographic patterns and ecological correlates of the mean genus age of liverworts in regional floras across China
Authors: Qian Hong , Dai Zun , Wang Jian 
Published: July 2023

AbstractAimMean lineage age is an important metric of evolutionary history and community structure of biological assemblages. Many studies have investigated geographic patterns and ecological drivers of mean lineage age in plant assemblages but all previous studies focused on vascular plants. Similar studies for bryophytes, a lineage of the oldest land plants, are lacking. Here, we fill this critical knowledge gap by investigating geographic patterns and ecological correlates of the mean genus age (MGA) of liverworts, a major lineage of bryophytes, in regional floras across China.LocationChina.TaxonLiverworts (hepatics, Division Marchantiophyta of bryophytes).MethodsMGA and its standardized effect size (MGAses) of liverworts in each of the 28 province‐level regions across China were related to 12 explanatory variables representing geography, contemporary climate and historical climate change, using correlation and regression analyses, and a variation partitioning approach.ResultsWe found that (1) MGA and MGAsesof liverworts were correlated negatively with latitude and elevational range, and positively with mean annual temperature, and annual precipitation in the regions across China, (2) MGA and MGAsesof liverworts were more strongly affected by temperature‐related variables than by precipitation‐related variables, and (3) MGA and MGAsesof liverworts were more strongly affected by climate extremes than by climate seasonality.Main ConclusionsThe relationships of mean lineage age with climatic condition in one of the oldest lineages of land plants (i.e. liverworts) are consistent with those in angiosperms, a relatively young lineage of land plants. Our study is the first to show that geographic and ecological distributions of the oldest land plants are generally consistent with the pattern frequently observed in angiosperms that biological assemblages in colder and drier climates have, on average, younger lineage ages.
Comparative evaluation of pinhole surgical technique with and withoutA‐PRFin the treatment of multiple adjacent recession defects: A clinico radiographic study
Authors: Trivedi Divya S. , Kolte Abhay P. , Kolte Rajashri A. , Deshpande Neha M. 
Published: July 2023

AbstractBackgroundTo evaluate and compare efficacy of pinhole surgical technique (PST) alone and with advanced platelet rich fibrin (A‐PRF) in the management of bilateral multiple adjacent gingival recession defects (MAGRD).MethodsOne hundred and sixty five MAGRD were randomly assigned to control group (treated with PST) and test group (PST with A‐PRF). Clinical parameters of gingival recession depth (GRD), gingival recession width (GRW), width of keratinised gingiva (WKG), complete root coverage (CRC) and gingival thickness (GT) on ST‐CBCT was measured at 2, 4 and 6 mm apically from the gingival margin. Also, root coverage aesthetic score and patient satisfaction ratings were recorded at baseline, 6 and 12 months postoperatively.ResultsSubstantial reduction in GRD (Test: 1.29 ± 0.69 mm and Control 0.98 ± 0.30 mm) (p < 0.001) and GRW (Test: 2.03 ± 0.90 mm and control 1.73 ± 0.99 mm) (p < 0.05) with associated gain in WKG and GT was observed (p < 0.001). Mean GT values were increased in both the groups at 2, 4 and 6 mm from the crest. Comparison of Test and Control groups yielded significant reductions in GRD (−0.17 ± 0.56 mm) and WKG (0.73 ± 1.07 mm) favoring the Test group (p < 0.05). Similar increase in GT was observed with better results in Test than control group. (p < 0.001).ConclusionBoth groups exhibited sound clinical outcomes with test group offering better resolution of MAGRD in comparison to control group. Also, it enhances clinical and therapeutic end results in terms of attaining reduction in GRD and GRW along with greater gain in KTW and GT.Clinical SignificancePST as a minimally invasive approach has numerous benefits, some of which include the absence of scarring and improved aesthetics linked to faster wound healing. The addition of A‐PRF enhances the intended therapy outcomes, which is beneficial for both patients and professionals in the field of periodontics.
Prediction of CO2 and ethylene produced in‐packaged apricot under cold plasma treatment by machine learning approach
Authors: Rashvand Mahdi , Altieri Giuseppe , Abbaszadeh Rouzbeh , Matera Attilio , Genovese Francesco , Feyissa Aberham Hailu , Di Renzo Giovanni Carlo 
Published: July 2023

AbstractIn this study, the fresh and in‐packaged apricots were treated with dielectric barrier discharge (DBD) cold plasma for 5, 10, and 15 min and then stored at 21°C for 12 days to simulate the shelf life of the apricot. The effects of DBD treatment on the main quality attributes of apricot such as physicochemical traits (mass loss, pH, soluble solid content, titratable acidity, and skin color), mechanical properties (Young's modulus, tangent modulus, and bioyield stress), in‐package gas composition and ethylene production were investigated during the storage time. In addition, the bruise susceptibility (BS) of control and treated samples at the microscale was evaluated by using pendulum test and scanning electron microscopy. The results of the mass loss, pH, soluble solid content, titratable acidity, skin color, and bioyield stress have been applied for input parameters of the developed artificial neural network (ANN) and support vector regression (SVR) models to predict the CO2 and ethylene production. The statistical data showed the performance of the developed ANN to predict the CO2 (R2 = 0.983, root mean square error [RMSE] = 0.486) and ethylene production (R2 = 0.933, RMSE = 5.376) was superior to SVR (CO2: R2 = 0.894, RMSE = 6.077 and ethylene: R2 = 0.759, RMSE = 14.117). These results indicated that intelligent methods are effective and robust tools for predicting the quality parameters of fresh fruits in the postharvest process.Practical ApplicationsAdvancement of technology and artificial intelligence leads to the tendency of many food industry engineers and researchers in the R&D department to use this technology to classify and predict various food industry processes. In the process of packaging products, there are challenges such as the amount of produced carbon dioxide and ethylene inside the package, which affects the shelf life of fresh agricultural products. In this research, artificial neural networks and support vector machine methods have been used to predict the two mentioned parameters. This approach can be used for the digitalization of the packaging process of agricultural and horticultural products and reduce the number of experimental activities. In addition, it is possible to improve the digitalization process by the fusion of smart sensors with machine learning methods.
Effect of PAR irradiance intensity on Phaeocystis antarctica (Prymnesiophyceae) growth and DMSP, DMSO, and acrylate concentrations
Authors: Kinsey Joanna D. , Tyssebotn Inger Marie B. , Kieber David J. 
Published: July 2023

AbstractPhaeocystis antarctica forms extensive spring blooms in the Southern Ocean that coincide with high concentrations of dimethylsulfoniopropionate (DMSP), dimethylsulfoxide (DMSO), dimethylsulfide (DMS), and acrylate. We determined how concentrations of these compounds changed during the growth of axenic P. antarctica cultures exposed to light‐limiting, sub‐saturating, and saturating PAR irradiances. Cellular DMSP concentrations per liter cell volume (CV) ranged between 199 and 403 mmol · LCV−1, with the highest concentrations observed under light‐limiting PAR. Cellular acrylate concentrations did not change appreciably with a change in irradiance level or growth, ranging between 18 and 45 mmol · LCV−1, constituting an estimated 0.2%–2.8% of cellular carbon. Both dissolved acrylate and DMSO increased substantially with irradiance during exponential growth on a per‐cell basis, ranging from 0.91 to 3.15 and 0.24 to 1.39 fmol · cell−1, respectively, indicating substantial export of these compounds into the dissolved phase. Average cellular DMSO:DMSP ratios increased 7.6‐fold between exponential and stationary phases of batch growth, with a 3‐ to 13‐fold increase in cellular DMSO likely formed from abiotic reactions of DMSP and DMS with reactive oxygen species (ROS). At mM levels, cellular DMSP and acrylate are proposed to serve as de facto antioxidants in P. antarctica not regulated by oxidative stress or changes in ROS. Instead, cellular DMSP concentrations are likely controlled by other physiological processes including an overflow mechanism to remove excess carbon via acrylate, DMS, and DMSO during times of unbalanced growth brought on by physical stress or nutrient limitation. Together, these compounds should aid P. antarctica in adapting to a range of PAR irradiances by maintaining cellular functions and reducing oxidative stress.
The Role of Explicit Memory Across Second Language Syntactic Development: A Structural Priming Study
Authors: Coumel Marion , Muylle Merel , Messenger Katherine , Hartsuiker Robert J. 
Published: July 2023

AbstractWe tested whether second language (L2) learners rely more on explicit memory during structural priming at lower than at higher proficiency levels (Hartsuiker & Bernolet, 2017). We compared within‐L2 priming with lexical overlap in 100 low and 100 high proficiency French L2 speakers under low versus high working memory load conditions induced with a letter series recall task presented between primes and targets. The high load condition would prevent explicit recall of primes during target production. Both groups primed more under low than high load. The effect of load was similar across groups, but exploratory analyses with proficiency as a continuous variable suggested that, with increasing proficiency, participants primed less under high load. We discuss how these findings support the idea that learners exploit explicit memory more during priming in early versus later stages of acquisition. Overall, this study showed that explicit memory influences syntactic processing across the L2 learning trajectory.
Which managerial reforms facilitate public sector innovation?
Authors: Raudla Ringa , Mohr Zachary , Douglas James W. 
Published: July 2023

AbstractA key question of public sector innovation (PSI) scholarship is: which factors influence innovation? This paper focuses on managerial practices as drivers of PSI and addresses two research questions. First, how have the main types of managerial reforms—pertaining to marketization, results‐orientation, and collaboration—influenced PSI? Second, how have different features of public sector reform strategies influenced PSI? Using survey data from 19 European countries, we show that reforms focusing on collaboration and results‐orientation facilitate PSI, while marketization‐type reforms have no significant impact. Our study indicates that reforms initiated by public administration (rather than politicians) are more conducive to PSI. We also show that reforms that are crisis‐driven and reforms oriented toward cost cutting have negative impacts on PSI. Overall, our findings demonstrate that New Public Governance‐type reforms exert more positive influence on PSI than NPM‐type reforms.
Plasma collection and supply in Europe: Proceedings of an International Plasma and Fractionation Association and European Blood Alliance symposium
Authors: Domanović Dragoslav , von Bonsdorff Leni , Tiberghien Pierre , Strengers Paul , Hotchko Matthew , O'Leary Peter , Thibert Jean‐Baptiste , Magnussen Karin , Erikstrup Christian , Spekman Marloes , Chesneau Stuart , Jones Judy , Møller Bjarne K. , Verheggen Peter , Gogarty Gerard , Elzaabi Mazen , de Angelis Vincenzo , Candura Fabio , Mali Polonca , Rossi Françoise , Rodrigues Bernardo , Sepetiene Ramune , Lenzen Thomas , Walsemann Stephan , Perry Robert , Plançon Jean‐Philippe , So‐Osman Cynthia , Durand‐Zaleski Isabelle , Facco Giuseppina , Thijssen‐Timmer Daphne 
Published: July 2023

AbstractAt the symposium organized by the International Plasma and Fractionation Association and European Blood Alliance, experts presented their views and experiences showing that the public sector and its blood establishments may strengthen the collection and increase the supply of plasma using the right strategies in plasma donor recruitment, retention and protection, scaling‐up collection by increasing the number of donors within improved/new infrastructure, supportive funding, policies and legislation as well as harmonization of clinical guidelines and the collaboration of all stakeholders. Such approaches should contribute to increased plasma collection in Europe to meet patients’ needs for plasma‐derived medicinal products, notably immunoglobulins and avoid shortages. Overall, presentations and discussions confirmed that European non‐profit transfusion institutions are committed to increasing the collection of plasma for fractionation from unpaid donors through dedicated programmes as well as novel strategies and research.
International Olympic Committee (IOC) consensus paper on sports-related ophthalmology issues in elite sports
Authors: Moe Morten Carstens , Özmert Emin , Baudouin Christophe , Binadra Abhinav , Crafoord Sven , Jo Young , Kiratli Hayyam , Moore Melita , Pitsiladis Yannis P , Rolle Udo , Tan Ben , Yanik Özge , Budgett Richard , Erdener Ugur , Steffen Kathrin , Engbretsen Lars 
Published: July 2023

Vision plays an important role in an athletes’ success. In sports, nearly 80% of perceptual input is visual, and eye health and sports medicine are closely intertwined fields of utmost importance to athletes. The physical nature of sports activities renders individuals more prone to various eye injuries than the general population. Ocular trauma can lead to lifelong sequelae, and impaired vision requires careful follow-up and management. Apart from injuries, athletes may also experience vision problems that can hamper their performance, including blurred vision, double vision, and light sensitivity.The interdisciplinary nature of sports medicine necessitates collaboration between sports medicine professionals and ophthalmologists. Through such collaborations, athletes can receive appropriate eye care, education on proper eye protection and guidance on adopting good eye health practices. If any inconspicuous symptoms are not detected and treated promptly, athletes may acquire systemic injuries because of defective vision, preventing them from achieving high level athletic performance in competitions.The protection of the elite athlete is the responsibility of all of us in sports medicine. To advance a more unified, evidence-informed approach to ophthalmic health assessment and management in athletes and as relevant for sports medicine physicians, the International Olympic Committee Consensus Group aims for a critical evaluation of the current state of the science and practice of ophthalmologic issues and illness in high-level sports, and present recommendations for a unified approach to this important issue.
Dual inhibition of BTLA and PD-1 can enhance therapeutic efficacy of paclitaxel on intraperitoneally disseminated tumors
Authors: Sun Wei-Zen , Lin Han-Wei , Chen Wan-Yu , Chien Chung-Liang , Lai Yen-Ling , Chen Jung , Chen Yu-Li , Cheng Wen-Fang 
Published: July 2023

BackgroundExpression of immune checkpoints in the tumor microenvironment is one mechanism underlying paclitaxel (PTX) chemoresistance. This study aimed to investigate whether the addition of checkpoint blockade to PTX can improve the therapeutic efficacy against apparently disseminated intraperitoneal tumors.MethodsWe analyzed the in vivo expression of various immune checkpoints in CD3+CD8+cytotoxic T cells from tumor-bearing mice treated with or without PTX and validated the tumor-killing activities of selected checkpoint-expressing T-cell subpopulations ex vivo. The regulation of selected checkpoints was investigated in vitro. The therapeutic effects of inhibition of a targeted checkpoint pathway with antibodies added to PTX therapy were examined.ResultsCD3+CD8+T cells expressed with herpes virus entry mediator (HVEM), programmed cell death 1 (PD-1), and T-cell immunoglobulin domain and mucin domain 3 (TIM-3) in tumor-bearing hosts treated with PTX had effective tumoricidal activities. In addition to PTX and cytokines, B and T lymphocyte attenuator (BTLA) or homologous to lymphotoxin, exhibits inducible expression and competes with herpes simplex virus (HSV) glycoprotein D for binding to HVEM, a receptor expressed on T lymphocytes (LIGHT) interacting with HVEM can regulate the expression of PD-1 on CD3+CD8+T cells. Interleukin (IL)-15 increased the percentage of HVEMhighgranzyme B (GZMB)+cells among CD3+CD8+T cells, which was suppressed by the BTLA/HVEM signal. LIGHT induced the percentage of HVEM+GZMB+cells but not HVEMhighGZMB+cells among CD3+CD8+T cells. Expression of IL-15, BTLA, or LIGHT was detected in CD19+B cells and regulated by damage-associated molecular patterns/Toll-like receptor interactions. In the tumor-bearing hosts treated with PTX, certain proportions of BTLA+B or PD-1+T lymphocytes were still noted. When dual inhibition of BTLA and PD-1 was added to PTX, the antitumor effects on intraperitoneally disseminated tumors can be significantly improved.ConclusionsDual blockade of BTLA on B cells and PD-1 on cytotoxic T cells may have clinical potential for enhancing the efficacy of PTX in the treatment of tumors with intraperitoneal spread, including epithelial ovarian carcinomas.
Cholesterol Efflux Drives the Generation of Immunosuppressive Macrophages to Promote the Progression of Human Hepatocellular Carcinoma
Authors: Li Zhixiong , Wang Yongchun , Xing Rui , Zeng Huilan , Yu Xing-Juan , Zhang Yao-jun , Xu Jing , Zheng Limin 
Published: July 2023

Abstract
Cholesterol is often enriched in tumor microenvironment (TME); however, its impact on disease progression varies in different tissues and cells. Monocytes/macrophages (Mφ) are major components and regulators of the TME and play pivotal roles in tumor progression and therapeutic responses. We aimed to investigate the profile, effects, and regulatory mechanisms of Mφ cholesterol metabolism in the context of human hepatocellular carcinoma (HCC). Here, we found that patients with high serum levels of cholesterol had shorter survival times and lower response rates to anti–PD-1 treatment. However, the cholesterol content in tumor-infiltrating monocytes/Mφ was significantly lower than that in their counterparts in paired nontumor tissues. The expression of the cholesterol efflux transporter, ABCA1, was upregulated in tumor monocytes/Mφ, and ABCA1 upregulation positively associated with decreased cellular cholesterol content and increased serum cholesterol levels. Mechanistically, autocrine cytokines from tumor-treated monocytes increased LXRα and ABCA1 expression, which led to the generation of immature and immunosuppressive Mφ. Although exogenous cholesterol alone had little direct effect on Mφ, it did act synergistically with tumor-derived factors to promote ABCA1 expression in Mφ with more immunosuppressive features. Moreover, high numbers of ABCA1+ Mφ in HCC tumors associated with reduced CD8+ T-cell infiltration and predicted poor clinical outcome for patients. Our results revealed that dysregulated cholesterol homeostasis, due to the collaborative effects of tumors and exogenous cholesterol, drives the generation of immunosuppressive Mφ. The selective modulation of cholesterol metabolism in Mφ may represent a novel strategy for cancer treatment.
Inhibition of Pyk2 Improves Cx43 Intercalated Disc Localization, Infarct Size, and Cardiac Function in Rats With Heart Failure
Authors: Zheng Li , Spagnol Gaelle , Gandhi Devashri R. , Sharma Kanika , Kumar Vikas , Patel Kaushik P. , Sorgen Paul L. 
Published: July 2023

BACKGROUND:
Heart failure causes changes in Cx43 (Connexin43) regulation that are associated with arrhythmic heart disease. Pyk2 (proline-rich tyrosine kinase 2) is activated in cardiomyopathies and phosphorylates Cx43 to decrease intercellular communication. This study was designed to determine if Pyk2 inhibition improves cardiac function in a myocardial infarction (MI)-induced heart failure model in rats.

METHODS:
MI (ligation of left anterior descending artery) rats were treated with the Pyk2 inhibitor PF4618433. Hemodynamic and structural parameters were monitored in Sham (n=5), MI-vehicle (n=5), and MI-PF4618433 (n=8) groups. Heart tissues were collected after 6 weeks to assess Pyk2 and Cx43 protein level and localization.

RESULTS:

PF4618433 produced no observed adverse effects and inhibited ventricular Pyk2. PF4618433 reduced the MI infarct size from 34% to 17% (
P=
0.007). PF4618433 improved stroke volume (
P=
0.031) and cardiac output (
P=
0.009) in comparison to MI-vehicle with values similar to the Sham group. PF4618433 also led to an increase in the ejection fraction (
P
=0.002) and fractional shortening (
P
=0.006) when compared with the MI-vehicle (32% and 35% improvement, respectively) yet were lower in comparison with the Sham group. Pyk2 inhibition decreased Cx43 tyrosine phosphorylation (
P
=0.043) and maintained Cx43 at the intercalated disc in the distal ventricle 6 weeks post-MI.

CONCLUSIONS:
Unlike other attempts to decrease Cx43 remodeling after MI-induced heart failure, inhibition of Pyk2 activity maintained Cx43 at the intercalated disc. This may have aided in the reduced infarct size (acute time frame) and improved cardiac function (chronic time frame). Additionally, we provide evidence that Pyk2 is activated following MI in human left ventricle, implicating a novel potential target for therapy in patients with heart failure.
Mosaic Loss of Chromosome Y Is Associated With Functional Outcome After Ischemic Stroke
Authors: Dorvall Malin , Pedersen Annie , Dumanski Jan P. , Söderholm Martin , Lindgren Arne G. , Stanne Tara M. , Jern Christina 
Published: July 2023

BACKGROUND:
Mosaic loss of chromosome Y (LOY) is associated with cardiovascular and neurodegenerative diseases in men, and genetic predisposition to LOY is associated with poor poststroke outcome. We, therefore, tested the hypothesis that LOY itself is associated with functional outcome after ischemic stroke.

METHODS:
The study comprised male patients with ischemic stroke from the cohort studies SAHLSIS2 (Sahlgrenska Academy Study on Ischemic Stroke Phase 2; n=588) and LSR (Lund Stroke Register; n=735). We used binary logistic regression to analyze associations between LOY, determined by DNA microarray intensity data, and poor 3-month functional outcome (modified Rankin Scale score, >2) in each cohort separately and combined. Patients who received recanalization therapy were excluded from sensitivity analyses.

RESULTS:

LOY was associated with about 2.5-fold increased risk of poor outcome in univariable analyses (
P
<0.001). This association withstood separate adjustment for stroke severity and diabetes in both cohorts but not age. In sensitivity analyses restricted to the nonrecanalization group (n=987 in the combined cohort), the association was significant also after separate adjustment for age (odds ratio, 1.6 [95% CI, 1.1–2.4]) and when additionally adjusting for stroke severity and diabetes (odds ratio, 1.6 [95% CI, 1.1–2.5]).

CONCLUSIONS:
We observed an association between LOY and poor outcome after ischemic stroke in patients not receiving recanalization therapy. Future studies on LOY and other somatic genetic alterations in larger stroke cohorts are warranted.