bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis
Authors: Ali Zienab , Dishisha Tarek , El-Gendy Ahmed O. , Azmy Ahmed F. 
Published: February 2023

Abstract
Background
Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics.

Results
Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively.

Conclusion
Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms.
The use of deformation imaging in the assessment of patients pre and post transcatheter aortic valve implantation
Authors: Coyle Mark , King Gerard , Bennett Kathleen , Maree Andrew , Hensey Mark , O’Connor Stephen , Daly Caroline , Murphy Gregory , Murphy Ross T. 
Published: February 2023

Abstract
Background
Deformation imaging represents a method of measuring myocardial function, including global longitudinal strain (GLS), peak atrial longitudinal strain (PALS) and radial strain. This study aimed to assess subclinical improvements in left ventricular function in patients undergoing transcatheter aortic valve implantation (TAVI) by comparing GLS, PALS and radial strain pre and post procedure.

Methods
We conducted a single site prospective observational study of 25 patients undergoing TAVI, comparing baseline and post-TAVI echocardiograms. Individual participants were assessed for differences in GLS, PALS and radial strain in addition to changes in left ventricular ejection fraction (LVEF) (%).

Results
Our results revealed a significant improvement in GLS (mean change pre-post of 2.14% [95% CI 1.08, 3.20] p = 0.0003) with no significant change in LVEF (0.96% [95% CI − 2.30, 4.22], p = 0.55). There was a statistically significant improvement in radial strain pre and post TAVI (mean 9.68% [95% CI 3.10, 16.25] p = 0.0058). There was positive trend towards improvements in PALS pre and post TAVI (mean change of 2.30% [95% CI − 0.19, 4.80] p = 0.068).

Conclusion
In patients undergoing TAVI, measuring GLS and radial strain provided statistically significant information regarding subclinical improvements in LV function, which may have prognostic implications. The incorporation of deformation imaging in addition to standard echocardiographic measurements may have an important role in guiding future management in patients undergoing TAVI and assessing response.
Interim analysis of the prospective COTRIMS (Cologne Trial of Retroperitoneal Lymphadectomy in Metastatic Seminoma) trial.
Authors: Heidenreich Axel , Seelemeyer Felix , Paffenholz Pia , Pfister David A. 
Published: February 2023

409 Background: Radiation therapy or systemic chemotherapy with 3 cycles PEB represent the guideline recommended treatment options in marker negative clinical stage IIA/B seminoma. Despite a high cure rate of 90% to 94% and 82% to 90% in CS IIA and IIB, respectively, both therapeutic options are associated with significant long-term toxicities. It was the aim of our trial to evaluate the feasibility, oncological efficacy and treatment associated morbidity of primary nsRPLND in stage IIA/B seminoma. Methods: 30 patients with marker negative clinical stage IIA and IIB classical seminoma of the testis were recruited in the prospective trial. Primary goal was a relapse rate <20% within 2 years follow-up. Exclusion criteria were adjuvant carboplatin therapy for clinical stage I disease, extensive clinical stage IIb or clinical stage IIC disease, previous retroperitoneal surgery or radiation therapy, and positive tumor markers. All patients underwent nerve sparing RPLND with a modified template resection. None of the patients received adjuvant chemotherapy in the presence of positive lymph nodes. All patients underwent close follow-up with imaging studies and tumor markers at 3-month intervals during the first 2 years, at 6-month intervals in year 3 and annually thereafter. 10 pts had preoperative serum analysis of miR371. Results: Mean age was 34.2 (21-54) years. Mean follow-up is 29.4 (1-60) months and 32.5 (3-60) for those with a minimum follow-up of 3 months. All patients were treatment-naïve; 19 and 11 patients were diagnosed with stage IIA and IIB disease, respectively, at time of RPLND. 28 and 2 patients underwent open and robot assisted ns RPLND, respectively. Mean OR time was 131 (105-195) min, mean blood loss was < 150ml and the mean hospitalization time was 4.5 (3-9) days. We did not observe surgery associated complications > Clavien Dindo grade 3a. Mean number of dissected lymph nodes was 18 (7-57), the mean number of positive lymph nodes was 1.3 (1-2) and the mean diameter of positive nodes was 2.3 (0.8-4.1) cm. Histology of the resected lymph nodes revealed metastatic seminoma in 25 (80%) patients; 2 and 3 patients demonstrated embryonal carcinoma and benign disease, resp. 10 pts underwent serum analysis of miR371 preoperatively which predicted metastatic disease in 9/10 and benign histology in 1/1. 2/30 (6.7%) patients developed an outfield relapse 4 and 6 months postoperatively. Both patients were salvaged by systemic chemotherapy with 4 cycles PEB. Conclusions: NsRPLND results in a high cure rate at midterm follow-up and it is associated with a low frequency of treatment associated morbidities making this approach a feasible alternative to standard radiation therapy or systemic chemotherapy. MiR371p might be a useful marker to predict presence/absence of metastases in equivocal findings. Clinical trial information: DRKS00025384 .
A review of selected psychotherapies for PTSD, their efficacy and treatment guidelines in adults
Authors: Kowalski Joachim , Elżanowski Adam , Śliwerski Andrzej 
Published: February 2023

CelOkoło 2.5% mieszkańców Polski będzie miało w ciągu życia pourazowe zaburzenia stresowe (PTSD). Ostatnie wydarzenia na świecie, pandemia i wojna w Ukrainie, stanowią czynniki, które zwiększą liczbę osób zmagających się z tym zaburzeniem. Dlatego celem niniejszej pracy jest podsumowanie i przybliżenie dostępnych dowodów naukowych dotyczących skuteczności stosowanych w Polsce psychoterapii PTSD.Materiał i metodyPrzegląd metaanaliz randomizowanych badań z grupą kontrolną oraz przegląd najaktualniejszych zaleceń terapeutycznych (treatment guidelines) dotyczących terapii PTSD.WynikiNajlepsze dostępne dowody wskazują na wysoką skuteczność terapii poznawczo-behawioralnej (CBT) opartej na ekspozycji przedłużonej i terapii Eye Movement Desensitization and Reprocessing (EMDR). Pewną skuteczność w zakresie PTSD ma również terapia humanistyczna, ale mniejszą od terapii opartych o ekspozycję na bodźce i wspomnienia związane z traumą. Brakuje dowodów potwierdzających skuteczność terapii psychodynamicznej i terapii opartych o teorię poliwagalną. Organizacje przygotowujące zalecenie terapeutyczne rekomendują przede wszystkim stosowanie CBT i EMDR.WnioskiSkuteczna psychoterapia PTSD powinna obejmować protokół postępowania zawierający komponentę ekspozycji na traumatyczne bodźce i wspomnienia. Zaleca się stosowanie tego rodzaju metod w psychoterapii PTSD.
Standard −20 °C freezer storage protocols may cause substantial plasma renin cryoactivation
Authors: Bonnitcha Paul , Rigdwell Mark , Ward Peter , Chesher Douglas 
Published: February 2023

Abstract

Objectives
To assess the appropriate preanalytical process for storage of plasma for renin concentration analysis. This study was initiated due to the wide variation in preanalytical handling of samples observed within our network, particularly with respect to freezing for longer term storage.

Methods
Pooled plasma from patient samples was analysed immediately post separation for renin concentration (n=30, concentration 4.0–204 mIU/L). Aliquots from these samples were frozen in a −20 °C freezer and then analysed, with the renin concentration compared to the respective baseline concentration. Comparisons were also made to: aliquots snap frozen using a dry ice/acetone bath, aliquots stored at room temperature, and aliquots stored at 4 °C. Subsequent experiments investigated the potential sources of cryoactivation observed in these initial studies.

Results
Substantial and highly variable cryoactivation was observed in samples frozen using a −20 °C freezer, with renin concentration increasing over 300% from baseline in some samples (median 21.3%). This cryoactivation could be prevented by snap freezing samples. Subsequent experiments determined that long term storage in a −20 °C freezer could prevent cryoactivation provided samples were initially frozen rapidly in a −70 °C freezer. Rapid defrosting of samples was not required to prevent cryoactivation.

Conclusions
Standard −20 °C freezers may not be appropriate for freezing samples for renin analysis. Laboratories should consider snap freezing their samples using a −70 °C freezer or similar to avoid cryoactivation of renin.
Systematic review and meta-analysis of within-subject and between-subject biological variation data of coagulation and fibrinolytic measurands
Authors: Hollestelle Martine J. , Kristoffersen Ann Helen , Idema René N. , Meijer Piet , Sandberg Sverre , de Maat Moniek P.M. , Aarsand Aasne K. 
Published: February 2023

Abstract

Objectives
The diagnosis and monitoring of bleeding and thrombotic disorders depend on correct haemostatic measurements. The availability of high-quality biological variation (BV) data is important in this context. Many studies have reported BV data for these measurands, but results are varied. The present study aims to deliver global within-subject (CVI) and between-subject (CVG) BV estimates for haemostasis measurands by meta-analyses of eligible studies, by assessment with the Biological Variation Data Critical Appraisal Checklist (BIVAC).

Methods
Relevant BV studies were graded by the BIVAC. Weighted estimates for CVI and CVG were obtained via meta-analysis of the BV data derived from BIVAC-compliant studies (graded A–C; whereby A represents optimal study design) performed in healthy adults.

Results
In 26 studies BV data were reported for 35 haemostasis measurands. For 9 measurands, only one eligible publication was identified and meta-analysis could not be performed. 74% of the publications were graded as BIVAC C. The CVI and CVG varied extensively between the haemostasis measurands. The highest estimates were observed for PAI-1 antigen (CVI 48.6%; CVG 59.8%) and activity (CVI 34.9%; CVG 90.2%), while the lowest were observed for activated protein C resistance ratio (CVI 1.5%; CVG 4.5%).

Conclusions
This study provides updated BV estimates of CVI and CVG with 95% confidence intervals for a wide range of haemostasis measurands. These estimates can be used to form the basis for analytical performance specifications for haemostasis tests used in the diagnostic work-up required in bleeding- and thrombosis events and for risk assessment.
Investigation on structural, magnetic and optical properties of Sm–Co Co-substituted BiFeO3 samples
Authors: Sharma Sheetal , Kumar Manoj 
Published: February 2023

Abstract
Sol–gel derived Sm–Co co-substituted BiFeO3 ceramics (Bi1–x
SmFe1–x
Co
x
O3 with x = 0.0, 0.01, 0.02 and 0.03; named as BFO, BSFCO-1, BSFCO-2 and BSFCO-3, respectively) were investigated for structural, vibrational, magnetic and optical properties. Distorted perovskite rhombohedral structure with R3c crystal symmetry has been established in X-ray diffraction (XRD) patterns analysis by Rietveld refinement and detailed structural parameters like lattice constants, unit cell volume, bond angles, bond length etc. have been evaluated. Raman spectra further confirmed typical rhombohedral structure of BiFeO3 by exhibiting 13 clear Raman active phonon (9E + 4A) modes along with second order modes in the wave number range 50–1500 cm−1. Fourier Transform Infrared (FTIR) spectra showed the presence of Fe–O and Bi–O bands and the calculated Fe–O bond length was in good agreement with that obtained from Rietveld analysis. Room temperature magnetization versus magnetic field (M–H) measurement using Vibrating Sample Magnetometer (VSM) showed enhancement of ferromagnetic ordering parameters with increasing Sm–Co content in BiFeO3 samples. The maximum magnetization values increased from 0.237 emu g−1 for BFO sample to 1.167 emu g−1 for BSFCO-3 sample along with increase in remnant magnetization values. The optical property of Bi1–x
SmFe1–x
Co
x
O3 samples was investigated by estimating the energy band gap using UV–Visible spectroscopy. The calculated values of energy band gap were varied in the range 2.46 eV–1.81 eV indicating tuning of energy band gap with Sm–Co co-substitution in BiFeO3 sample.
Respiratory function monitoring during early resuscitation and prediction of outcomes in prematurely born infants
Authors: Gunawardana Shannon , Arattu Thodika Fahad M.S. , Murthy Vadivelam , Bhat Prashanth , Williams Emma E. , Dassios Theodore , Milner Anthony D. , Greenough Anne 
Published: February 2023

Abstract

Objectives
Over the last decade, there has been increased use of end-tidal carbon dioxide (ETCO2) and oxygen saturation (SpO2) monitoring during resuscitation of prematurely born infants in the delivery suite. Our objectives were to test the hypotheses that low end-tidal carbon dioxide (ETCO2) levels, low oxygen saturations (SpO2) and high expiratory tidal volumes (VTE) during the early stages of resuscitation would be associated with adverse outcomes in preterm infants.

Methods
Respiratory recordings made in the first 10 min of resuscitation in the delivery suite of 60 infants, median GA 27 (interquartile range 25–29) weeks were analysed. The results were compared of infants who did or did not die or did or did not develop intracerebral haemorrhage (ICH) or bronchopulmonary dysplasia (BPD).

Results
Twenty-five infants (42%) developed an ICH and 23 (47%) BPD; 11 (18%) died. ETCO2 at approximately 5 min after birth was lower in infants who developed an ICH, this remained significant after adjusting for gestational age, coagulopathy and chorioamnionitis (p=0.03). ETCO2 levels were lower in infants who developed ICH or died compared to those that survived without ICH, which remained significant after adjustment for gestational age, Apgar score at 10 min, chorioamnionitis and coagulopathy (p=0.004). SpO2 at approximately 5 min was lower in the infants who died compared to those who survived which remained significant after adjusting for the 5-min Apgar score and chorioamnionitis (p=0.021).

Conclusions
ETCO2 and SpO2 levels during early resuscitation in the delivery suite were associated with adverse outcomes.
Validation of interpretation of ST-elevation myocardial infarction using the smartphone based 12 lead electrocardiograms in comparison to the cardiologist-a cross sectional study, validation study, single-center study
Authors: Mahajan Sahil , Garg Salil , Sharma Richa , Singh Yogendra , Chandola Nitin , Bhatia Tanuj , Bansal Basundhara 
Published: February 2023

Background: In patients experiencing an ST-elevation myocardial infarction (STEMI), rapid diagnosis leads to optimal clinical outcomes. Recent technology has provided access to a reliable means of obtaining an ECG reading through a smartphone application (app) that works with an attachment providing all 12-leads of a standard ECG system. We developed a 12-lead smartphone-based electrocardiogram (ECG) called “Spandan”, and an application to accurately assess the presence of STEMI in patients presenting with chest pain. Objectives of study was to perform clinical observation studies on the patients screened for STEMIs and evaluate the variation in the diagnosis of STEMIs detected in both 12-lead gold standard and Spandan 12 lead ECG.
Methods: This single-center study was carried out at Shri Mahant Indresh hospital (SMIH), Dehradun, Uttarakhand, India from April-2022 to November-2022. All patients (n=278) visiting the ECG room with symptoms of chest pain, shortness of breath and past history of cardiovascular disease at the department of cardiology of the SMIH, Dehradun were included in the study.
Results: Mean age (SD) was 53.96±15.31 years. The male gender participants was 75.17%. 14.3% were STEMI patients as per the standard 12 lead interpretation, smartphone ECG interpreted 7.1% of the participants as STEMI and cardiologist interpreted nearly 15.4% of the participants of STEMI.
Conclusions: This study confirmed the potential of a smartphone ECG for evaluation of STEMI and the feasibility of studying this technology further to define the diagnostic accuracy, limitations, and appropriate use of this new technology.
Reliability and assessment of partial or complete anterior cruciate ligament injuries with diagnostic arthroscopy
Authors: Chintapalli Surya Sri Karun , Praveen Narayan  , Pothuri Rishi Ram  , Bhaktharahalli Ramchandran Harshavardhan 
Published: February 2023

Background: Partial tears of anterior cruciate ligament (ACL) were more commonly identified in young group with knee twisting. Partial tears are characterized by tear involvement of less than 50% of the ligament is torn. The arthroscopic evaluation is considered as gold standard in diagnostic evaluation. The comparison of findings between partial and complete tears in magnetic resonance imaging (MRI) and arthroscopy helps in finding out reliability of diagnostic modalities and consider the surgeon to establish the plan of treatment.
Methods: 2-year prospective study at the department of orthopedics, RRMCH, Bangalore, India. 40 consecutive patients with suspicion of cruciate injuries of knee were selected for the study from July 2018 to May 2021. The MR imaging of knee in selected cases were performed and the findings were obtained. The descriptive statistical analysis was prepared which includes spectrum of MRI findings in cruciate injuries of knee that were correlated with arthroscopy findings in determining the accuracy.
Results: Total 40 patients, we obtained 100% sensitivity and 66.67% specificity, 93.75% accuracy of MRI with arthroscopy in diagnosing complete ACL tears. We obtained 90.91% sensitivity and 80% specificity, 87.50% accuracy of MRI with arthroscopy in diagnosing partial ACL tears.
Conclusions: The partial tears can be missed or can be over diagnosed on MRI. These misinterpretations are more likely to happen due to intra-substance signal abnormality. Description of the status of ACL ligament in arthroscopy helped the orthopaedic surgeons in decision making as conservative approach in partial tears and reconstruction in a complete tear.
The carbon dioxide fluxes at the open-top chambers experiment on the ombrotrophic bog (Mukhrino field station)
Authors: Zarov E. A. , Jacotot A. , Kulik A. A. , Gogo S. S. , Lapshina E. D. , Dyukarev E. A. 
Published: February 2023

The continuous measurement of CO2 fluxes at the open-top chamber experiment in the ombrotrophic peatland (located in the middle taiga zone, West Siberia, Russia) has been provided during the warm season of 2022 (beginning of June to beginning of October). The Reco, NEE and GPP were calculated for this period; abiotic factors related to CO2 emissions, such as PAR, air temperature, water table level and precipitation, were also measured. The monthly average values showed a negative NEE of -9.89 C g m-2 month-1 in July, a negative GPP of -34.19 C g m-2 month-1 in July, and a positive values Reco of 41.68 C g m-2 month-1 in August. In 2022, the studied peatland hollows were only a carbon stock in July, while in the remaining months they were a source of CO2, which could be caused by small precipitation amount.
The monthly average diurnal variations of CO2 fluxes showed similar behaviour for both the OTC plot and control plot fluxes, which may be explained by the similarity in vegetation cover.
Congratulations! Journal of Robotics and Mechatronics Best Paper Award 2022
Authors:   Editorial Office
Published: February 2023

300-N Class Convex-Based Telescopic Manipulator and Trial for 3-DOF Parallel Mechanism Robot
We are pleased to announce that the 15th Journal of Robotics and Mechatronics Best Paper Award (JRM Best Paper Award 2022) has been decided by the JRM editorial committee. The following paper won the JRM Best Paper Award 2022, severely selected from among all 123 papers published in Vol.33 (2021). The Best Paper Award ceremony was held on December 23, 2022 in hybrid style (both on-site and online; venue: Gakushi-Kaikan, Tokyo, Japan), attended by the authors and JRM editorial committee members who took part in the selection process. The award winners were given certificates and a nearly US$1,000 honorarium. Editorial committee members who participated online also congratulated them through Zoom. We congratulate the winners and sincerely wish them success in the future.
Takashi Kei Saito, Kento Onodera, Riku Seino, Takashi Okawa, and Yasushi Saito
Journal of Robotics and Mechatronics, Vol.33, No.1, pp. 141-150, February 2021
doi: 10.20965/jrm.2021.p0141