bluuebunny/crossref_metadata_2025_split huggingface dataset:
Car-borne Survey and Dose Assessment from External Radiation Exposure in Bangka Island
Authors: Pradana Radhia , Nugraha Eka Djatnika , Wahyudi Wahyudi , Untara Untara , Wiyono Muji , Devriany Ade , Shilfa Sharah Nataz , Sasaki Michiya , Prasetio Heru , Winarni Ilma Dwi , Ekaranti Egnes , Nuraeni Nunung , Kranrod Chutima , Iskandar Dadong , Suhariyono Gatot , Surniyantoro Harry Nugroho Eko , Makhsun Makhsun , Widodo Susilo , Omori Yasutaka , Hiswara Eri , Hosoda Masahiro , Yoshinaga Shinji , Tokonami Shinji 
Published: May 2023

Abstract
Bangka Island with a history of more than 200 years of tin mining, has brought along the byproduct of heavy minerals which contain radionuclide elements. There are some concerns about this byproduct material contributing to natural radiation in the environment. In this study, a car-borne survey was conducted, to give an accurate assessment of natural background radiation in Bangka Island. The measurement of indoor and outdoor ambient dose rates in 146 houses was also conducted for the assessment of radiation dose from external exposure received by the public. The soil samples were collected and measured by a gamma spectroscopy system to find specific radionuclide contributions to external terrestrial exposure. From 3790 measurement points during the car-borne survey, the highest ambient dose equivalent rate was measured at 596 nSv h−1 found in Muntok area, while the mean value was 101 nSv h−1 and the median value was 95 nSv.h−1. The ambient dose equivalent rate distribution map shows a relatively higher value in the northern coastal area of the island, where the Pemali tin deposit was located. The annual effective doses received from external radiation in 146 houses of Bangka ranged from 0.44 mSv y−1 to 1.30 mSv y−1, with a median value of 0.66 mSv y−1. The soil contains a relatively higher amount of thorium (232Th) which contributes 75% to external radiation exposure in Bangka Island.
Influence of chemical nature of citric and malic acids on reaction time of the crosslinking of polyvinyl alcohol hydrogels
Authors: de Castro Dione Pereira , Kieffer Vanessa Zimmer , Santana Ruth Marlene Campomanes 
Published: May 2023

Abstract
This work studied the incorporation of organic acids as crosslinking agents and reaction time on the crosslinked properties of poly(alcohol vinyl) (PVOH) hydrogels. PVOH hydrogels crosslinked with citric and L-malic acids were prepared, and the effects of heat-treatment time, and temperature on their swelling and hygroscopic performances were investigated by kinetics of water uptake, gel fraction, thermal analysis and FTIR. Both the swelling and rate of water uptake of hydrogels decreased with increasing heat-treatment time. While the swelling decreased with heat-treatment time, the chemical crosslinking shown in FTIR increased. DSC results indicated adsorbed water in the uncrosslinked PVOH and hydrogels, and the absorbed water changed the melting point and glass transition temperature. TGA analysis showed that the incorporation of organic acids caused more thermal stability. Analysis revealed that the heat treatment time and the initial crosslinking time increased the concentration of ester groups with a corresponding decrease in hydroxyl groups.
Vaginal bacteria elicit acute inflammatory response in fallopian tube organoids: a model for pelvic inflammatory disease
Authors: Yu Bo , McCartney Stephen , Strenk Susan , Valint Daniel J. , Liu Congzhou , Haggerty Catherine , Fredricks David 
Published: May 2023

Abstract
Objective: To facilitate in vitro mechanistic studies in pelvic inflammatory disease (PID) and subsequent tubal factor infertility, as well as ovarian carcinogenesis, we sought to establish patient tissue derived fallopian tube (FT) organoids and to study their inflammatory response to acute vaginal bacterial infection.
Design: Experimental study.
Setting: Academic medical and researchcenter.
Patients: FT tissues were obtained from four patients after salpingectomy for benign gynecological diseases.
Interventions: We introduced acute infection in the FT organoid culture system by inoculating the organoid culture media with two common vaginal bacterial species, Lactobacillus crispatus and Fannyhesseavaginae.
Main Outcome Measures: The inflammatory response elicited in the organoids after acute bacterial infection was analyzed by the expression profile of 249 inflammatory genes.
Results: Compared to the negative controls that were not cultured with any bacteria, the organoids cultured with either bacterial species showed multiple differentially expressed inflammatory genes. Marked differences were noted between the Lactobacillus crispatus infected organoids and those infected by Fannyhessea vaginae. Genes from the C-X-C motif chemokine ligand (CXCL) family were highly upregulated in F. vaginae infected organoids. Flow cytometry showed that immune cells quickly disappeared during the organoid culture, indicating the inflammatory response observed with bacterial culture was generated by the epithelial cells in the organoids.
Conclusion: Patient tissue derived FT organoids respond to acute bacterial infection with upregulation of inflammatory genes specific to different vaginal bacterial species. FT organoids is a useful model system to study the host-pathogen interaction during bacterial infection which may facilitate mechanistic investigations in PID and its contribution to tubal factor infertility and ovarian carcinogenesis.
Rates of textbook outcome achieved in patients undergoing liver and pancreatic surgery
Authors: Villodre Celia , Alcázar-López Cándido , Melgar Paola , Carbonell-Morote Silvia , Franco-Campello Mariano , Rubio-García Juan Jesús , Rodríguez-Laiz Gonzalo , Ramia José Manuel 
Published: May 2023

Abstract
Textbook outcome (TO) is a composite measure that reflects the most desirable surgical results as a single indicator. The aim of this study was to assess the achievement of TO at a hepatopancreatobiliary (HPB) surgery unit in a Spanish tertiary hospital.
Methods:
Retrospective observational study of all consecutive patients who underwent HPB surgery over a 4-year period. Morbidity (defined according to the Clavien-Dindo classification) and mortality at 30 days, hospital stay, risk of morbidity and mortality according to the POSSUM scale and readmissions at 90 days were recorded. TO was considered when a patient presented no major complications (³IIIA), no mortality, no readmission, and not prolonged length of stay (LOS: £ 75th percentile of the sample).
Results:
A total of 283 patients were included (median age: 65 years, 65.4% men). Morbidity >IIIA was reported in 21.6%, and 5.7% died; median postoperative stay was 4 days. TO was achieved in 56.2% of patients. Comparing patients that presented TO with those that did not, significant differences were recorded for the type of procedure and the expected risk of morbidity and mortality calculated according to the POSSUM scale. There were significant differences between patients with major resections (TO rates of 46.3% in major hepatectomy and 52.5% in major pancreatectomy) and those with minor resections (rates of 67.7% in minor hepatectomy and 40.7% in minor pancreatectomy).
Conclusion:
The rate of TO in our series (56.2%) was like those published internationally. TO is a very useful management tool for assessing postoperative results.
Assessing the relationship between the annual average temperature and the indexes of annual average maximum , minimum temperature and wind speed in China based on the use of weighted empirical likelihood for semi-parametric varying coefficient partially linear models with heteroscedastic error
Authors: Wu Lin , Wang Xiang , Xie Ai Fang 
Published: May 2023

Abstract
The change of annual mean temperature generally has profound impact on our environment and decision-makers. Based on meterological data used for analysis in the past 63 years from 1951 to 2013, the weighted empirical likelihood for varying coefficient partially linear models with heteroscedastic errors is used to obtain the relationship between the index of the annual minimum climate temperature , the annual maximum climate temperature , the wind speed and annual climate temperature . Furthermore, we conclude that some meaningful characteristics for the relationship between the studied climate indices are obtained. Assisted by some statistical indexes of R 2 , we conclude that we get the goodness of the fit of the regression relationship based on the given data set with the method of weighted empirical likelihood for semi-parametric varying coefficient partially linear models with heteroscedastic error . At the same time, we have obtained that for the case of heteroscedastic data, the estimator of parametric with the empirical likelihood method with heteroscedastic data has the shorter confidence than the empirical likelihood method neglecting heteroscedastic factor.
Serum metabolomic analysis in positive-TgAb or positive-TPOAb in female Hashimoto's thyroiditis patients
Authors: Jiang Xiao , Zhao Xinyu , Luo Tao , Li Pengqian , Gu Xiaotong , Wan Chuchu , He Mengfan , Bian Xiaolong , Liu Haixia 
Published: May 2023

Abstract
Background
TgAb and TPOAb are effective and sensitive diagnose index for HT which is a common AID.TgAb and TPOAb present varying degrees correlatuion with different metabolites in different gender. However, the morbidity in female are higher than male in HT. The research in exploring the correction between metabolic and positive-TgAb or positive-TPOAb in female HT patients is still absent.
Methods
14 healthy, 14 TPOAb(+), 4 TgAb(+) patients serum sample were included. Metabolites were detected using the LC-MS. Sstatistical analysis were performed by SPSS. PLS-DA and OPLS-DA were carried by SIMCA. VIP > 1.5 metabolites by OPLS-DA were assessed for statistical significance by t-test or non-parametric test. Enrichment analysis and heatmap of metabolite were conducted by MetaboAnalyst. Correlation analysis was performed by Origin 2021. The ROC curve was established by SPSS. Metabolite point plotting was drawn by Graph prism 9.0.
Results
Based on VIP > 1.5 and P < 0.05 as selection criteria, 36 metabolites were derived. 13 metabolites were selected from the control vs the TPOAb (+) group, 23 metabolites were identified from the control vs the TgAb (+) group. TgAb and Phenylacetyl-L-glutamine / TPOAb and LPC 16:0 sn-1 performed strong correlation the TPOAb (+) group. Furthermore, TPOAb and LPE 16:1 was presented correlation the TgAb (+) group. Enrichment analysis of metabolic pathways showed that Glycine, serine and threonine metabolism was significant in TPOAb (+), while Galactose metabolism in TgAb (+) group.
Conclusion
The level of serum metabolites in TPOAb(+) TgAb(-) female patients and TPOAb(-) TgAb(+) female patients are different.
Source reconstruction of clinical resting-state EEG reveals differences in power and functional connectivity in children with developmental dyslexia
Authors: Garnica-Agudelo David , Smith Stuart D.W. , de Velden Daniel van , Stier Christina , Brockmann Knut , Schroeder Sascha , Neef Nicole E. , Focke Niels K. 
Published: May 2023

Abstract
Background: Developmental dyslexia is a neurodevelopmental disorder characterized by significant difficulties in reading and spelling. Despite lacking routine neuroimaging markers for dyslexia, recent resting-state electroencephalography (EEG) studies have detected atypical functional connectivity in children with dyslexia compared to controls. These methods are based on measures of EEG data at a sensor-level, but it remains unclear if routine clinical resting-state EEG can be used to detect source-level differences in power or functional connectivity (FC) between children with dyslexia and controls. It is also unknown if differences in these EEG metrics correlate with difficulties in reading and spelling.
Methods: Using retrospective data, we investigated the source-reconstructed power and FC of 70 children with recently diagnosed dyslexia and 50 typically developing controls. We analyzed 50 seconds of awake resting-state routine clinical EEG in five frequency bands (1-29 Hz) using power, imaginary part of coherency (ImCoh), and weighted phase lag index (wPLI). Additionally, we calculated correlations between power or FC and IQ, reading, and spelling performance.
Results: Children with dyslexia had a decrease in theta FC in left temporo-parieto-occipital regions and an increase in alpha FC in left fronto-temporo-parietal regions. A decrease of theta FC was observed for right parieto-occipital regions and an increase of alpha FC in right inferior fronto-temporal regions. Furthermore, children with dyslexia demonstrated lower power in delta and theta within the left parieto-occipital regions. An age-stratified sub-analysis indicated that children with dyslexia in 5th-8th school grades exhibit greater alpha FC mainly in left fronto-temporo-parietal regions. Finally, lower scores in spelling showed a positive and significant association to theta power within left parieto-occipital regions in dyslexia.
Conclusions: Significant group differences in power and FC in the theta-alpha range in left cortical language and visual regions, as well as in multiple resting-state networks (RSNs), suggest abnormal oscillations as a pathophysiological sign of dyslexia reading and spelling deficits. These findings demonstrate the potential of source-reconstructed clinical routine EEG data to inform clinicians about brain network alterations in neurodevelopmental disorders such as dyslexia.
A joint profile of cytokines and immune cell populations may identify HNSCC patients who benefit from nivolumab treatment. The Gruppo Oncologico Nord-Ovest (GONO) study Nivactor T-2
Authors: Merlano Marco Carlo , Paccagnella Matteo , Denaro Nerina , Abbona Andrea , Galizia Danilo , Sangiolo Dario , Gammaitoni Loretta , Fiorino Erika , Minei Silvia , Bossi Paolo , Licitra Lisa , Garrone Ornella 
Published: May 2023

Abstract

Background: Immunotherapy of head and neck cancer induces a limited but reproducible rate of long-term survivors, at the cost of treating a large number of patients exposed to toxicity without benefit, regardless of PD-L1 expression. Therefore, identification of better markers for response is an unmet need.Materials and methods: 18 cytokines and 24 subpopulations of immune cells, selected on their prevalent Th1 or Th2 effect, were collected from peripheral blood. Samples were gathered at baseline (T0) and after 3 courses of nivolumab (T1) in 22 head and neck cancer patients, refractory to platinum containing therapy or in second line treatment for relapsed/metastatic disease. Data extracted at each time point have been linked to overall survival. A threshold value able to discriminate between good or poor survival, have been identified by ROC analysis. The relative value of the most promising cytokines/immune cells was determined by PCA.Results:at T0, 4 cytokines (IL-6, IL-8, IL-10, TGF-β) and 2 immune cells (CD3+CD8+LAG3+, CD3+CD11+HLA-DRlowCD14-) were able to discriminate between good and poor survival and allowed the identification of two clusters of patients.Conclusion:with the limitation of an exploratory analysis, this report suggests that a mixed profile of cytokine and immune cells determined at baseline, is potentially able to discriminate between patients who will benefit from nivolumab treatment and those who will do not.
Prognostic and Predictive Value of Endothelial Dysfunction Biomarkers in Sepsis-Associated Acute Kidney Injury: Risk Stratified Analysis from a Prospective Observational Cohort of Pediatric Septic Shock
Authors: Atreya Mihir R. , Cvijanovich Natalie Z. , Fitzgerald Julie C. , Weiss Scott L. , Bigham Michael T. , Jain Parag N. , Schwarz Adam J. , Lutfi Riad , Nowak Jeffrey , Allen Geoffrey L. , Thomas Neal J. , Grunwell Jocelyn R. , Baines Torrey , Quasney Michael , Haileselassie Bereketeab , Alder Matthew N. , Goldstein Stuart L. , Stanski Natalja L. 
Published: May 2023

Abstract
Background
Sepsis Associated Acute Kidney Injury (SA-AKI) is associated with high morbidity, with no current therapies available beyond continuous renal replacement therapy (CRRT). Systemic inflammation and endothelial dysfunction are key drivers of SA-AKI. We sought to measure differences between endothelial dysfunction markers among children with and without SA-AKI, test whether this association varied across inflammatory biomarker-based mortality risk strata and develop prediction models to identify those at highest risk of SA-AKI.
Methods
Secondary analyses of prospective observational cohort of pediatric septic shock. Primary outcome of interest was presence ≥ Stage II KDIGO SA-AKI on day 3 (D3 SA-AKI). Biomarkers including those prospectively validated to predict pediatric sepsis mortality (PERSEVERE-II) were measured in Day 1 (D1) serum. Multivariable regression was used to test the independent association between endothelial markers and D3 SA-AKI. We conducted risk-stratified analyses and developed prediction models using Classification and Regression Tree (CART), to estimate risk of D3 SA-AKI and prespecified subgroups based on PERSEVERE-II risk.
Results
414 patients were included in the derivation cohort. Patients with D3 SA-AKI had worse clinical outcomes including 28-day mortality and need for CRRT. Serum soluble thrombomodulin (sTM), Angiopoietin-2 (Angpt-2), and Tie-2 were independently associated with D3 SA-AKI. Further, Tie-2 and Angpt-2/Tie-2 ratios were influenced by the interaction between D3 SA-AKI and mortality risk strata. Logistic regression demonstrated models predictive of D3 SA-AKI risk performed optimally among patients with high- or intermediate- PERSEVERE-II mortality risk strata. A 6 terminal node CART model restricted to this subgroup of patients had an area under the receiver operating characteristic curve (AUROC) 0.90 and 0.77 upon 10-fold cross-validation in the derivation cohort to distinguish those with and without D3 SA-AKI and high specificity. The newly derived model performed well in a unique set of patients (n = 224), 84 of whom were deemed high- or intermediate- PERSEVERE-II risk, to distinguish those patients with high vs. low risk of D3 SA-AKI.
Conclusions
Endothelial dysfunction biomarkers are independently associated with risk of severe SA-AKI. Pending validation, incorporation of endothelial biomarkers may facilitate prognostic and predictive enrichment for selection of therapeutics in future clinical trials among critically ill children.
G-quadruplex structural dynamics at MAPK12 promoter dictates transcriptional switch to determine stemness in breast cancer.
Authors: Sengupta Pallabi , Dutta Anindya , Suseelaa Yelisetty Venkata , Roychowdhury Tanaya , Banerjee Nilanjan , Dutta Ananya , Halder Satyajit , Jana Kuladip , Mukherjee Gopeswar , Chattopadhyay Samit , Govindaraju Thimmaiah , chatterjee subhrangsu 
Published: May 2023

Abstract
p38γ (MAPK12) is predominantly expressed in triple negative breast cancer cells (TNBC) and induces stem cell (CSC) expansion resulting in decreased survival of the patients due to metastasis. Abundance of G-rich sequences at MAPK12 promoter implied the functional probability to reverse tumorigenesis, though the formation of G4 structures at MAPK12 promoter is elusive. Here, we identified two evolutionary consensus adjacent G-Quadruplex (G4) motifs upstream MAPK12 promoter, forming parallel G4 structures. They exist in competitive binding equilibria between G4 and duplex, regulated by the binding turnover of Sp1 and Nucleolin, binding to different non-contiguous sites at G4 motifs and sequestering either’s occupancy at these motifs to poise MAPK12 transcriptional homeostasis. To under-score the gene-regulatory functions of G4 motifs, we employed CRISPR-Cas9 system to eliminate G4s from TNBC cells and synthesized a naphthalene diimide (NDI) derivative (TGS24) which shows high-affinity binding to MAPK12-G4 and inhibits MAPK12 transcription. Deletion of G4 motifs and NDI compound interfere with the recruitment of the transcription factors, inhibiting MAPK12 expression in cancer cells. The molecular basis of NDI-induced G4 transcriptional regulation was analyzed by RNA-seq analyses, which revealed that MAPK12-G4 inhibits oncogenic RAS transformation and trans-activation of NANOG. MAPK12-G4 also reduces CD44+/CD24- population in TNBC cells and downregulates internal stem cell markers, arresting the stemness properties of cancer cells.
False Springs in The Netherlands: Climate Change Impact Assessment with the False Spring Damage Indicator Model
Authors: Bosdijk Joep Jesse , de Feiter Vincent Simon , Gaiser Annika , Smink Thijs Andreas Gerardus , Thorkelsdottir Gudrun , Vliet Arnold J.H. van , Luedeling Eike 
Published: May 2023

Abstract

Strong night frosts in spring have the potential to cause severe damage to fruit trees, causing up to €3.3 billion of damage in Europe in 2017. If temperatures drop below -2.2°C after the growing season starts, this phenomenon is referred to as a False Spring. For The Netherlands, the risk of false spring damage increased during the past 70 years, due to the dates of the last strong night frost and the start of blooming moving closer together. This raises the question whether this trend will continue under future influences of climate change. To analyse the development of false spring damage risks, we developed the False Spring Damage Indicator (FSDI) model. Applying this model to the Dutch KNMI'14 climate change scenarios for 2050 and 2085 showed a decrease in false spring damage risk. The historical risk for 60% bud damage was reduced to around 40% for the moderate scenario in 2050, and to less than 15% for the severe scenario in 2050 and all scenarios in 2080. False spring damage varied spatially throughout The Netherlands. Currently, in the east more severe damage can be expected, while coastal regions in the north and west are less prone to false spring damage. In the future, this is expected to become more uniform over The Netherlands, as the risk in the east is predicted to decrease strongly, becoming similar to the low damage risk in the coastal regions.
Nurse-led virtual prostate cancer clinic for survivorship care: Qualitative study of patient experiences and needs
Authors: Xiong Ting , Young Karen , Pfisterer Kaylen , Ng Denise , Lohani Raima , Jiao Tina , Nunn Caitlin , Bryant-Lukosius Denise , Rendon Ricardo , Berlin Alejandro , Bender Jacqueline , Brown Ian , Feifer Andrew , Gotto Geoffrey , Cafazzo Joseph , Pham Quynh 
Published: May 2023

Abstract
Background: Prostate cancer survivors (PCa) can experience a range of unmet needs over a long horizon of survivorship. Thin healthcare services and specialist-led follow-up care models may not adequately address these needs. Digitally mediated nurse-led virtual care models may help support PCa survivors who have unmet survivorship needs in current healthcare systems.
Methods: This qualitative descriptive study explores 10 patients’ experiences with follow-up and virtual care, informing the design of a nurse-led virtual clinic (the “Ned Nurse” Clinic) to provide integrated healthcare services.
Results: Patient follow-up care experiences included uncertainty regarding care gaps despite new telemedicine modalities and the need for personalized wellbeing support. Patient recommendations for virtual care related to improving integration of existing care connections, supporting patient self-management, and addressing accessibility. Patients anticipate nurse-led PCa virtual care will: (1) clarify patient and provider roles and responsibilities; (2) improve care ease and access to care; (3) enhance mental wellbeing, and (4) reinforce continuity of care. Conclusion: Patients are keen to benefit from the flexibility and increased resources that digital health may provide, but remain concerned about digital literacy and service changes to support these innovations. Future work should evaluate the efficacy of digitally mediated nurse-led virtual care models to support PCa survivorship.