bluuebunny/crossref_metadata_2025_split huggingface dataset:
IGF2BP2 promotes colorectal cancer progression by upregulating the expression of TFRC and enhancing iron metabolism
Authors: Liu Tian-yue , Hu Chen-chen , Han Chen-ying , Mao Si-yi , Zhang Wen-xin , Xu Yi-ming , Sun Yuan-jie , Jiang Dong-bo , Zhang Xi-yang , Zhang Jia-xing , Wang Jing , Qiao Xu-peng , Pan Jing-yu , Yang Shu-ya , Yang Kun 
Published: April 2023

Abstract
Background
Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive system, ranking third for morbidity and mortality worldwide. At present, no effective control method is available for this cancer type. In tumor cells, especially iron metabolization, is necessary for its growth and proliferation. High levels of iron are an important feature to maintain tumor growth; however, the overall mechanism remains unclear.

Methods
We used western blotting, immunohistochemistry (IHC) and real-time quantitative PCR to analyze the expression of IGF2BP2 in cell lines and tissues. Further, RNA-sequencing, RNA immunoprecipitation and methylated RNA immunoprecipitation experiments explored the specific binding of target genes. Moreover, the RNA stability assay was performed to determine the half-life of genes downstream of IGF2BP2. In addition, the Cell Counting Kit-8, colony formation assay, 5-ethynyl-2’-deoxyuridine assay and flow cytometry were used to evaluate the effects of IGF2BP2 on proliferation and iron metabolism. Lastly, the role of IGF2BP2 in promoting CRC growth was demonstrated in animal models.

Results
We observed that IGF2BP2 is associated with iron homeostasis and that TFRC is a downstream target of IGF2BP2. Further, overexpression of TFRC can rescue the growth of IGF2BP2-knockdown CRC cells. Mechanistically, we determined that IGF2BP2 regulates TFRC methylation via METTL4, thereby regulating iron metabolism and promoting CRC growth. Furthermore, using animal models, we observed that IGF2BP2 promotes CRC growth.

Conclusion
IGF2BP2 regulates TFRC mRNA methylation via METTL4, thereby regulating iron metabolism and promoting CRC growth. Our study highlights the key roles of IGF2BP2 in CRC carcinogenesis and the iron transport pathways.
Consensus on the management of united airways disease with type 2 inflammation: a multidisciplinary Delphi study
Authors: Blanco-Aparicio Marina , Domínguez-Ortega Javier , Cisneros Carolina , Colás Carlos , Casas Francisco , del Cuvillo Alfonso , Alobid Isam , Quirce Santiago , Mullol Joaquim 
Published: April 2023

Abstract
Background
Scientific evidence on patients with multimorbid type 2 asthma and chronic rhinosinusitis with nasal polyps (CRSwNP) from a united airways disease (UAD) perspective remains scarce, despite the frequent coexistence of these entities. We aimed to generate expert consensus-based recommendations for the management of UAD patients.

Methods
Using a two-round Delphi method, Spanish expert allergists, pulmonologists and otolaryngologists expressed their agreement on 32 statements (52 items) on a 9-point Likert scale, classified as appropriate (median 7–9), uncertain (4–6) or inappropriate (1–3). Consensus was considered when at least two-thirds of the panel scored within the range containing the median.

Results
A panel of 30 experts reached consensus on the appropriateness of 43 out of the 52 (82.7%) items. The usefulness of certain biomarkers (tissue and peripheral blood eosinophil count, serum total IgE, and fraction of exhaled nitric oxide [FeNO]) in the identification and follow-up of type 2 inflammation, and assessment of the response to biologics, were agreed. Some of these biomarkers were also associated with disease severity and/or recurrence after endoscopic sinus surgery (ESS). Consensus was achieved on treatment strategies related to the prescription of anti-IL-4/IL-13 or anti-IgE agents, concomitant treatment with systemic corticosteroids, and combining or switching to biologics with a different mechanism of action, considering a number of UAD clinical scenarios.

Conclusion
We provide expert-based recommendations to assist in clinical decision-making for the management of patients with multimorbid type 2 asthma and CRSwNP. Specific clinical trials and real-world studies focusing on the single-entity UAD are required to address controversial items.
Parental alienation in Lebanon: a case report
Authors: Fares Rabih , Najem Roudna , Hallit Souheil , Pelissolo Antoine , Haddad Georges , Naja Wadih J. 
Published: April 2023

Abstract
Background
Parental alienation is a relatively newly described disorder, with a growing prevalence, as divorce and custody battles are becoming more and more complex with increased difficulty of joint custody. In parental alienation, one parent, the alienating parent, forms an alliance with the child involved in the custody dispute and manages to effectively alienate the targeted parent completely. The child and the alienating parent manifest a form of folie à deux and, hence, are in complete synchrony in the hatred and denigration of the targeted parent. Issues, such as potentially false allegations of sexual, physical, and emotional abuse of the child by the targeted parent, arise. The child and the alienating parent become mutually convinced of the targeted parent’s transgressions. Consequently, it becomes difficult for the courts and psychiatric professionals to differentiate true abuse from parental alienation.

Case presentation
In this case study, we aimed to conduct an in-depth psychological and psychiatric evaluation of a Lebanese family (white race) where a father was wrongly accused by the mother and his 11-year-old white boy of both physical and sexual abuse. The data for this study were collected through unstructured and semi-structured interviews, observations, and psychological tests (Rorschach test for the parents and Blacky test for the child), and through the analysis of documented evidence presented in the trial.

Conclusion
This case manifested most criteria set forth for the diagnosis of parental alienation and created serious doubt regarding the validity of the allegations set forth by the mother and the child. Uncovered parental alienation often misleads mental health professionals at the expense of the child’s mental health .
In-silico target prediction by ensemble chemogenomic model based on multi-scale information of chemical structures and protein sequences
Authors: Yang Su-Qing , Zhang Liu-Xia , Ge You-Jin , Zhang Jin-Wei , Hu Jian-Xin , Shen Cheng-Ying , Lu Ai-Ping , Hou Ting-Jun , Cao Dong-Sheng 
Published: April 2023

AbstractIdentification and validation of bioactive small-molecule targets is a significant challenge in drug discovery. In recent years, various in-silico approaches have been proposed to expedite time- and resource-consuming experiments for target detection. Herein, we developed several chemogenomic models for target prediction based on multi-scale information of chemical structures and protein sequences. By combining the information of a compound with multiple protein targets together and putting these compound-target pairs into a well-established model, the scores to indicate whether there are interactions between compounds and targets can be derived, and thus a target prediction task can be completed by sorting the outputted scores. To improve the prediction performance, we constructed several chemogenomic models using multi-scale information of chemical structures and protein sequences, and the ensemble model with the best performance was used as our final model. The model was validated by various strategies and external datasets and the promising target prediction capability of the model, i.e., the fraction of known targets identified in the top-k (1 to 10) list of the potential target candidates suggested by the model, was confirmed. Compared with multiple state-of-art target prediction methods, our model showed equivalent or better predictive ability in terms of the top-k predictions. It is expected that our method can be utilized as a powerful computational tool to narrow down the potential targets for experimental testing.
Graphical Abstract
Loss of Stat3 in Osterix+ cells impairs dental hard tissues development
Authors: Chan Laiting , Lu Jiarui , Feng Xin , Lin Lichieh , Yao Yichen , Zhang Xiaolei 
Published: April 2023

Abstract
Background
Mutations in the signal transducers and activators of transcription 3 (STAT3) gene result in hyper-IgE syndrome(HIES), a rare immunodeficiency that causes abnormalities in immune system, bones and teeth. However, the role of Stat3 in development of dental hard tissues was yet to investigate.

Methods
In this study, a transgenic mouse of conditional knockout of Stat3 in dental mesenchymal cells (Osx-Cre; Stat3fl/fl, Stat3 CKO) was made. The differences of postnatal tooth development between control and Stat3 CKO mice were compared by histology, µCT and scanning electron microscopy.

Result
Compared with the control, Stat3 CKO mice were presented with remarkable abnormal tooth phenotypes characterized by short root and thin dentin in molars and incisors. The enamel defects were also found on mandibular incisors. showed that Ki67-positive cells significantly decreased in dental mesenchymal of Stat3 CKO mice. In addition, β-catenin signaling was reduced in Hertwig's epithelial root sheath (HERS) and odontoblasts of Stat3 CKO mice.

Conclusions
Our results suggested that Stat3 played an important role in dental hard tissues development, and Stat3 may regulate dentin and tooth root development through the β-catenin signaling pathway.
Perceived barriers to seeking treatment for alcohol use disorders among the general Danish population – a cross sectional study on the role of severity of alcohol use and gender
Authors: Finn Sara Wallhed , Mejldal Anna , Nielsen Anette Søgaard 
Published: April 2023

Abstract
Background
A minority of all individuals with alcohol use disorders (AUD) seek treatment. Since the suffering from AUD has severe consequences for both the individual and for society, it is important to improve the understanding of barriers to seeking treatment. Most studies of barriers thus far have been conducted in the United States of America or the United Kingdom. There is a need for studies from other contexts. The overall aim is to investigate barriers to treatment seeking for AUD. The specific aims are to: 1) describe barriers to AUD treatment at different levels of alcohol use. 2) compare gender differences regarding barriers to AUD treatment.

Methods
Study design: Cross-sectional. Participants: 1594 representative Danish adults from the general population aged 30–65 years. An online questionnaire was administrated by a market research company. The questionnaire covered demographic data, barriers to treatment and level of alcohol use. Analyses were performed by means of chi-2 test and logistic regression.

Results
The most common barriers were related to stigma and shame: admitting to others of having a problem, being labelled, fear of the consequences and that others would find out. Participants with higher severity of alcohol use were more likely to endorse a wish to handle alcohol problems themselves and to report barriers related to treatment services. Women with high severity of alcohol use, endorsed higher level of fear of the consequences than men.

Conclusions
There is an urgent need to reduce stigma around AUD. Individuals with higher severity of alcohol use report a lower willingness to seek professional treatment if a problem occurs. Especially among individuals with high severity of alcohol use there is a need to address gender specific barriers.
Properties of Concrete and Structural Behaviour of Reinforced Concrete Beam Containing Shredded Waste Paper as An Additive
Authors: Solahuddin B. A. , Yahaya F. M. 
Published: April 2023

AbstractThis research uses WP to investigate the effect of two types of Shredded Waste Paper (SWP) comprising Shredded Copier Waste Paper (SCPWP) and Shredded Cardboard Waste Paper (SCBWP) as additives on the properties of concrete and the structural behaviour of Reinforced Concrete Beam (RCB). The slump, compressive, flexural, and splitting tensile strengths increase by 4–13% for 5–10% addition of SCPWP and decrease by 16–23% for 15% addition of SCPWP compared to 0% addition. For SCBWP, the slump, compressive, flexural and splitting tensile strengths increase by 10–23% for 5–10% addition and decrease by 15–21% for 15% addition compared to 0% addition. 15% of SCPWP and SCBWP addition records the highest effect in water absorption and efflorescence, showing 11% and 10.28% increases with 15% addition of SCBWP and SCPWP. Scanning electron microscope (SEM) analysis reveals that the crack is repaired, and the presence of calcium hydroxide (Ca(OH)2) and calcium–silicate–hydrate (C–S–H) links enhances the concrete strength. The addition of 10% SCPWP and 10% SCBWP in the concrete mixtures improves the structural behaviour of RCB with stirrup spacing (SS) = 100 mm (full), 150 mm and 200 mm (reduced) by increasing the load and reducing the deflection. Apart from that, the concrete bending and shear strains also increase by 44.17% and 34.9%. The failure mode of the RCB changes from shear to bending. This study indicates that SCPWP and SCBWP can be used as additives in concrete at 5% and 10%, and 10% for RCB with significant strength and structural improvement.
Comparison of LSM indexing techniques for storing spatial data
Authors: Mao Qizhong , Qader Mohiuddin Abdul , Hristidis Vagelis 
Published: April 2023

AbstractIn the pre-big data era, many traditional databases supported spatial queries via spatial indexes. However, modern applications are seeing a rapid increase of the volume and ingestion rate of spatial data. Log-structured Merge (LSM) tree is used by many big data systems as their storage structure in order to support write-intensive large-volume workloads, which are usually only optimized for single-dimensional data. Research has studied how spatial indexes can be supported on LSM systems, but focused mainly on the local index organization, that is, how data is organized inside a single LSM component. This paper studies various aspects of LSM spatial indexing, including spatial merge policies, which determine when and how spatial components are merged. Three stack-based and one leveled merge policies have been studied, which have been implemented on a common big data system Apache AsterixDB. The write and read performance on various workloads is evaluated, and our findings and recommendations are discussed. A key finding is that Leveled policies underperform other stack-based merge policies for most types of spatial workloads.
Longitudinal structures of zonal wind in the thermosphere by the ICON/MIGHTI and the main wave sources
Authors: Li Dan , Gao Hong , Xu Jiyao , Zhu Yajun , Xu Qiuyu , Liu Yangkun , Liu Hongshan 
Published: April 2023

AbstractIn this study, the neutral wind observations from the Michelson Interferometer for Global High-resolution Thermospheric Imaging (MIGHTI) instrument onboard Ionospheric CONnections (ICON) are used to investigate the longitudinal structure of zonal wind between 100 and 300 km during daytime. The four-peaked structure in the longitudinal distribution between June and August is visually clear in the whole altitudinal range. The longitudinal wavenumber 1–4 patterns (WN1–WN4) are extracted, and the altitude–month distributions of WN1–WN4 and their contributions to the longitudinal structure are compared. The amplitudes of WN3 and WN4 show seasonal dependence, and the amplitude of WN4 exhibits obvious vertical propagation from the mesosphere and lower thermosphere (MLT) to the upper thermosphere in summer and autumn. WN1 is an important contributor to the longitudinal structure, WN4 is the primary contributor in the lower altitude ranges in summer and autumn at three latitudes. The contributions of WN3 (WN1) increase holistically with latitude in summer (spring, autumn, and winter). And the main wave sources of WN1–WN4 are further investigated in the 100–106 km and 210–300 km altitude regions. The main wave sources of WN1 and WN2 have complex variations with altitude, latitude, and season, while WN3 (WN4) is clearly influenced by DE2 (DE3 and SE2).
Graphical Abstract
Strong ground motion simulations of the 2016 Kumamoto earthquakes using corrected empirical Green’s functions: methods and results for ESG6 blind prediction Steps 2 and 3 with improved parameters
Authors: Nagasaka Yosuke 
Published: April 2023

AbstractThis paper describes the methods and results of the strong ground motion simulations for three earthquakes from the 2016 Kumamoto earthquake sequence using corrected empirical Green’s functions. The target earthquakes were an aftershock (Mw 5.5), the largest foreshock of the sequence (Mw 6.1), and the mainshock (Mw 7.1). The corrected empirical Green’s function method was used in the simulations. This simulation method combines simple source and path factors with empirical site amplification and phase factors to generate realistic site-specific strong motions. Simulations were originally conducted to participate in blind prediction exercises in ESG6. Although the simulations performed in this study were based on the models submitted to the blind prediction committee, several modifications were made after the blind prediction exercise. First, the observed records at the target site of the blind prediction called KUMA were used to compare observed and synthetic strong ground motions. In addition, a regional spectral inversion was conducted to obtain a more appropriate Q-value and site amplification factor. Synthetic strong motions were found to explain the observed strong ground motions at KUMA and other stations. Comparisons with predictions by other methods and the sensitivity to the rupture scenario were also discussed. These results provide useful information for applying the corrected Green’s function method to strong ground motion simulations.
Graphical Abstract
Effect of low-to-moderate hyperoxia on lung injury in preclinical animal models: a systematic review and meta-analysis
Authors: Minkove Samuel , Dhamapurkar Rhea , Cui Xizhong , Li Yan , Sun Junfeng , Cooper Diane , Eichacker Peter Q. , Torabi-Parizi Parizad 
Published: April 2023

Abstract
Background
Extensive animal investigation informed clinical practice regarding the harmful effects of high fractional inspired oxygen concentrations (FiO2s > 0.60). Since questions persist whether lower but still supraphysiologic FiO2 ≤ 0.60 and > 0.21 (FiO2 ≤ 0.60/ > 0.21) are also harmful with inflammatory lung injury in patients, we performed a systematic review examining this question in animal models.

Methods
Studies retrieved from systematic literature searches of three databases, that compared the effects of exposure to FiO2 ≤ 0.60/ > 0.21 vs. FiO2 = 0.21 for ≥ 24 h in adult in vivo animal models including an inflammatory challenge or not were analyzed. Survival, body weight and/or lung injury measures were included in meta-analysis if reported in ≥ 3 studies.

Results
More than 600 retrieved reports investigated only FiO2s > 0.60 and were not analyzed. Ten studies with an inflammatory challenge (6 infectious and 4 noninfectious) and 14 studies without, investigated FiO2s ≤ 0.60/ > 0.21 and were analyzed separately. In seven studies with an inflammatory challenge, compared to FiO2 = 0.21, FiO2 ≤ 0.60/ > 0.21 had consistent effects across animal types on the overall odds ratio of survival (95%CI) that was on the side of harm but not significant [0.68 (0.38,1.23), p = 0.21; I2 = 0%, p = 0.57]. However, oxygen exposure times were only 1d in 4 studies and 2–4d in another. In a trend approaching significance, FiO2 ≤ 0.60/ > 0.21 with an inflammatory challenge consistently increased the standardized mean difference (95%CI) (SMD) in lung weights [0.47 (− 0.07,1.00), p = 0.09; I2 = 0%, p = 0.50; n = 4 studies] but had inconsistent effects on lung lavage protein concentrations (n = 3), lung pathology scores (n = 4) and/or arterial oxygenation (n = 4) (I2 ≥ 43%, p ≤ 0.17). Studies without an inflammatory challenge had consistent effects on lung lavage protein concentration (n = 3) SMDs on the side of being increased that was not significant [0.43 (− 0.23,1.09), p = 0.20; I2 = 0%, p = 0.40] but had inconsistent effects on body and lung weights (n = 6 and 8 studies, respectively) (I2 ≥ 71%, p < 0.01). Quality of evidence for studies was weak.

Interpretation
Limited animal studies have investigated FiO2 ≤ 0.60/ > 0.21 with clinically relevant models and endpoints but suggest even these lower FiO2s may be injurious. Given the influence animal studies examining FiO2 > 0.60 have had on clinical practice, additional ones investigating FiO2 ≤ 0.60/ > 0.21 appear warranted, particularly in pneumonia models.
Prepancreatic postduodenal portal vein: a case report and literature review
Authors: Akashi Masanori , Miyazaki Daiki , Hashimoto Kazuaki , Fukutomi Shogo , Arai Shoichiro , Goto Yuichi , Sato Toshihiro , Sakai Hisamune , Hisaka Toru 
Published: April 2023

Abstract
Background
Among congenital anomalies of the portal venous system, prepancreatic postduodenal portal vein (PPPV) is very rare and has only been reported to date. Herein, we report a case of PPPV identified in preoperative examinations for hepatocellular carcinoma and a literature review.

Case presentation
A 63-year-old man was admitted to our hospital for treatment of a liver tumor. After examination, he was diagnosed with hepatocellular carcinoma with a diameter of 40 mm in segment 8. Contrast-enhanced computed tomography scan showed a portal vein passing between the duodenum and pancreas, hence called PPPV. At the hepatic hilus, the portal vein branched off in a complicated course with some porto-portal communications. We determined that anatomical resection with manipulation of the hepatic hilum in this case resulted in major vascular injury. Therefore, we performed partial liver resection, and the patient was discharged uneventfully on postoperative day 14.

Conclusions
Although PPPV is an extremely rare congenital vascular variant, it is important to carefully identify vascular patterns preoperatively and to recognize the possibility of such an anomaly to avoid misidentification and inadvertent injuries during surgery.