bluuebunny/crossref_metadata_2025_split huggingface dataset:
Survival outcome of different treatment sequences in patients with locally advanced and metastatic pancreatic cancer
Authors: fan mengjiao , deng guochao , si haiyan , wang zhikuan , dai guanghai 
Published: January 2023

Abstract
Background: Chemotherapy remained the basic treatment for patients with advanced pancreatic cancer. We aimed to evaluate the impact of different treatment sequences on clinical outcomes of advanced pancreatic cancer at our academic institution.
Methods: In this single institution retrospective analysis, we assessed characteristics and survival rates from all patients with locally advanced and metastatic pancreatic ductal adenocarcinoma who started a systemic treatment between 01/2015 and 12/2021. Survival analyses were performed by Kaplan-Meier and Cox proportional hazards model.
Results: A total number of 285 patients started a systemic treatment in the observation period, and all patients received at least two lines of treatment. The completion rate of third-line chemotherapy was higher in patients who received FOLFIRINOX or mFLORIFINOX in third-line compared with the patients who received FOLFIRINOX regimen in the first or second-line (chi-square, p=0.04). Furthermore, survival rates of three lines (gemcitabine plus nab-paclitaxel or nab-paclitaxel combination therapy to FOLFIRINOX including plus Bevacizumab; nab-paclitaxel combination therapy to gemcitabine combination therapy, or cross, to oxaliplatin or irinotecan combination therapy; and nab-paclitaxel combination therapy to gemcitabine combination therapy, or cross, to FOLFIRINOX including plus Bevacizumab) were significantly different and median overall survival was 14.00, 14.00, and 18.00 months, respectively (p＜0.05).
Conclusion: Our study provides real-world evidence for the effectiveness of different treatment sequences and recommends nab-paclitaxel combination therapy to gemcitabine combination therapy, or cross, to FOLFIRINOX+/-Bevacizumab to achieve a best overall survival when considering the entire management in advanced pancreatic cancer.
Simultaneously evaluation of physiological and biochemical responses in the leaves and roots of Iranian common bermudagrass [Cynodon dactylon L. (Pers.)] accessions under a wide range of temperature fluctuations
Authors: Amini Zohreh , Salehi Hassan , Chehrazi Mehrangiz , Etemadi Mohammad 
Published: January 2023

AbstractThe average temperature of the earth's surface is increasing rapidly, negatively affecting the urban space's green cover. In this study, to identify Iranian common bermudagrass accessions which could endure a wide range of temperature fluctuations, and be identified as cold-heat tolerant accessions, seven cold-tolerant Iranian accessions including Taft, Naein, Malayer, Gardane-Heyran, Aligoudarz, Safashahr, and Gorgan along with Ahvaz accession as a native accession to tropical regions of Iran were subjected to five high-temperature regimes, including 35/30°C (control), 40/35°C (moderate heat stress), 45/40°C (severe heat stress), 50/45°C (extreme heat stress), and 50/50°C (high-extreme heat stress) day/night cycles for 21 days. At the end of this experiment, it was found that Gorgan and Safashahr accessions were able to endure the 50/45°C TR with acceptable turfgrass quality (heat-tolerant accessions). Ahvaz and Aligudarz accessions could withstand extreme heat stress, but their visual quality was weaker than the former group (semi-heat-tolerant). In contrast, Taft, Naein, Malayer and Gardane-Heyran accessions could not tolerate this harsh condition and their shoots were destroyed (sensitive accessions). Evaluation of physiological parameters (Tchl, RWC, EC and RV), osmolytes (proline, TSC and starch) and antioxidants (SOD, APX, CAT and POX) in leaves and roots after moderate heat stress showed that all parameters except RWC and root viability increased in all accessions. With rising temperature in the 45/40°C TR, root viability in sensitive, semi-tolerant and tolerant accessions increased by 170%, 99% and 143% respectively, and its rate in sensitive accessions was almost twice that of tolerant accessions, while there was no significant difference in the amount of stored starch in their roots. In the continuation of the experiment, when the heat stress became extreme, root viability in sensitive accessions was greatly reduced (89%) and its rate was almost 1/4 of that in tolerant accessions. In such a situation, sensitive accessions could only use 4% of their roots' starch reserves, while semi-tolerant and tolerant accessions used 35% and 47%, respectively. These findings provided that heat-tolerant plants with controlling respiratory rate in roots and efficiently breaking down starch storage to carbohydrates could provide the energy required for whole plant metabolic activities. This experiment also highlights the importance of simultaneously investigating evaluated parameters in leaves and roots.
Use Topic Modeling to Understand Comments in Student Evaluations of Teaching
Authors: Sun Jie , Yan Lu 
Published: January 2023

Abstract
Written comments in student evaluation of teaching offer a rich source of data for understanding instructors’ teaching and students’ learning experiences. However, most of previous researches on student evaluation of teaching have focused on the numeric ratings to close-ended questions, few researches tried to analyze the content of students’ written comments to open-ended questions, which normally involved a labor-intensive manual process of coding and categorizing. Such qualitative efforts prevent solutions on a large scale since it is almost impossible to go through all the textual data manually. Therefore, an innovative quantitative method that can analyze large corpus of data holds great promise. This paper proposed LDA method of topic modeling to discover important themes emerged in students’ written comments. We compared our results with findings in previous qualitative studies. We also investigated how these themes varied by course grade levels and course subjects. Our results provided first evidence that topic modeling can be an effective and efficient alternative to understand teaching and learning experiences through students’ written comments on a large scale.
An Attention-BiLSTM Model for Satellite Operation Prediction with Correlation Telemetry
Authors: Peng Yi , Jia Shuze , Xie Lizi , Shang Jian 
Published: January 2023

Abstract
At present, satellite anomaly is mostly solved after the event, and rarely predicted in advance in satellite health management. Thus, satellite trend prediction is quietly important for avoiding the fault which perhaps affects data accuracy and service quality of satellite, and even impacts greatly on satellite safety. However, it is difficult to predict satellite operation through a simple model because satellite system is complex, and telemetry data is numerous, coupled and spatiotemporal. Therefore, this paper proposes a model combing attention mechanism and Bidirectional Long Short-term Memory (Attention-BiLSTM) with correlation telemetry to predict the situation of satellite operation. Firstly, high-dimensional K-NearestNeighbor Mutual Information (HKNN-MI) method is performed to select the related telemetry variables from multiple variables of satellite telemetry data. Secondly, we put forward to a new BiLSTM model with attention mechanism for telemetry prediction. The dataset for the research is generated and transmitted from the power system of FY3E meteorological satellite. In order to verify the superiority of the proposed model, it is compared with other method based on the same dataset in the experiment. The result shows that the method outperforms other methods due to its better accuracy and prediction precision.
A case report of central adrenal insufficiency: hiding in persistent, refractory nausea and vomiting
Authors: Yang Jia , Wang Xiao , Tang Jin , Hu Ziling , Xu Tingting , Wang Hongwei , Wang Lei , Cheng Yanmei 
Published: January 2023

Abstract
Background
Central adrenal insufficiency (CAI) is defined as the inability of the adrenal glands to release sufficient corticosteroids due to a series of diseases or injuries of the hypothalamus or pituitary. Signs and symptoms of CAI are insidious, ambiguous, and debilitating. Preceding studies suggest that elderly patients with CAI may present with hyponatremia as a characteristic manifestation, but little is mentioned about gastrointestinal (GI) symptoms. Herein we report a rare case of an elderly male patient with a radio-chemotherapy history for nasopharyngeal carcinoma, in whom prolonged exogenous glucocorticoid replacement and infectious stress from two bouts of bacterial pneumonia combined to cause severe CAI with prodromal symptoms of persistent, intractable nausea and vomiting.
Case presentation
A 71-year-old man presented to the gastroenterology department with persistent nausea and vomiting. Gastroscopy, brain magnetic resonance imaging (MRI), and contrast-enhanced abdominal computed tomography (CT) were performed to exclude organic lesions. The diagnosis of CAI was confirmed by checking the levels of basal cortisol and adrenocorticotropic hormone. After replacement therapy with hydrocortisone, the patient's GI symptoms resolved rapidly, hyponatremia was corrected. At subsequent follow-ups, he was doing well with no hospitalizations.
Conclusion
CAI in elderly patients can start with persistent, refractory nausea and vomiting, and is featured by uncorrectable and insidious hyponatremia. Timely hydrocortisone replacement therapy averts life-threatening adrenal crises.
Study on Sequence Mutation and Molecular Dynamics Simulation of D614G SARS-CoV-2 Spike Protein in Indonesia
Authors: Rahmi Aulia Fauziyyah , Utomo Didik Huswo , Susanti R. 
Published: January 2023

Abstract
Amino acid mutations in the D614G spike protein of SARS-CoV-2 need to be studied more deeply for their influence on the pathogenesis of the virus. The purpose of this research was to analyze 1) the variation and kinship of the SARS-CoV-2 spike protein gene; 2) the effect of D614G spike protein mutation on ACE2 receptor binding and the molecular dynamics simulation. The nucleotide sequence of SARS-CoV-2 virus isolate was downloaded from GISAID and NCBI. Classification of SARS-CoV-2 clades and construction of the phylogenetic tree using MEGA-program. Molecular dynamics simulation was demonstrated with GROMACS to compare the stability of D614 and G614 S proteins. The result of this research showed that the most common GH clade SARS-CoV-2 virus isolates were be discovered in Indonesia, and the percentage of isolates with the D614G mutation reached 97% in Indonesia. The clade with the D614G mutation made the SARS-CoV-2 virus more varied with new mutations and increased human-to-human transmision. In this study, an increase in infectivity or spread of the SARS-CoV-2 D614G virus most likely resulted from an increase in the strength of the S protein and a slight change in RBD conformation seen from the molecular interactions around the mutation area and the RMSF value (0.3144 nm for D614G, and 0.3818 nm for D614). This is likely to be the cause of the massive spread of variants belonging to VOC and VBM, where all these variants have the D614G mutation.
Pyroptosis-related bioinformatics analysis reveals that IL1A is a prognostic biomarker in oral squamous cell carcinoma
Authors: Chen Gaoxiang , Shao Tingru , Lu Xinyan , Zheng Zenan , Lv Xiaozhi 
Published: January 2023

Abstract
Background: Increasing evidence has demonstrated that pyroptosis plays a crucial role in tumor carcinogenesis and progression. However, there is little information concerning pyroptosis-related genes (PRGs) in oral squamous cell carcinoma (OSCC). Purpose of our study was to explore the prognostic value of PRGs in OSCC patients.
Materials and methods: RNA-seq and clinical data were downloaded from The Cancer Genome Atlas (TCGA) database and PRGs were extracted from the expression profiles. Then, differentially-expressed analysis and functional enrichment analysis were performed. A prognostic model based on PRGs was constructed in R software. Moreover, comprehensive bioinformatics analyses were used to verify the prognostic model and select pyroptosis-related biomarkers.
Results: A total of 35 genes were categorized as differentially-expressed PRGs and Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis showed that these genes were mainly located in inflammasome complex and associated with pyroptosis. CHPM3, GSDMB, IL1A and NLRP1 were used to establish the prognostic model. Risk scores of each patients was calculated by using the risk-score formula and a nomogram was plotted to visually predict the survival of the OSCC patients. Then half-maximal inhibitory concentration (IC50) of 30 common anticancer drugs was analyzed between patients in high-risk and low-risk cohorts. In the end, IL1A was identified as potential pyroptosis-related biomarkers of OSCC.
Conclusion: This study established a novel pyroptosis-related prognostic model, provided a novel strategy for predicting the survival of OSCC patients. Moreover, IL1A was identified as an independent pyroptosi-related biomarker and may be a potential target for OSCC.
Male Infertility in Nigeria and South Africa: A Ten-Year Observational Study
Authors: Akang Edidiong Nnamso , Opuwari Chinyerum Sylvia , Enyioma-Alozie Swesme , Moungala Lionel Wildy , Amatu Tamarapreye Emmanuel , Wada Ibrahim , Ogbeche Rose Ogeyi , Ajayi Oluwatoyin Omolayo , Aderonmu Mojisola Modupe , Shote Olaitan Boluwatife , Akinola Lateef Adekunle , Ashiru Oladapo Adenrele , Henkel Ralf 
Published: January 2023

Abstract
Background: Male infertility is a disease of public health relevance contributing to 50% of infertility cases globally. However, there is dearth of information on the prevalence of male-factor infertility in Sub-Saharan countries. Therefore, in this study we aimed at identifying the prevalence, and trends of male factor infertility in Nigeria and South Africa over a period of ten years between 2010 and 2019.
Methods:A retrospective study of semen analyses of 17,292 men attending fertility hospitals in Nigeria and South Africa in 2010, 2015 and 2019. Patients who had undergone vasectomy and those who had a pH less than 5 or greater than 10 were excluded from this study. The following variables were assessed: ejaculate volume, sperm concentration, progressive motility, total progressively motile sperm count (TPMSC), total sperm count, and normal sperm morphology
Results:
Between 2010 and 2019, significant trends of decreasing values were observed in normal sperm morphology (-50%), progressive motility (-89%), TPMSC (-91%) and the ejaculatory volume (-7%), indicating a progressive deterioration of the values in both countries. In Nigeria, there were significant decreases in progressive motility (-87%), TPMSC (-78%), and sperm morphology (-50%) between 2015-2019 (p<0.0001). Spearman`s rank correlation revealed significant negative associations between age and morphology (ρ = -0.24, p<0.0001), progressive motility (ρ= - 0.31. p<0.0001), and TPMSC (ρ = -0.32, p<0.0001). Patients in South Africa were younger than those from Nigeria, with also a significantly higher sperm morphology, sperm concentration, progressive motility, total sperm count and TPMSC.
Conclusion: Our findings provide a quantitative evidence of an alarming decreasing trend in semen parameters in Nigeria and South Africa from 2010 to 2019. It also proves that astheno- and teratozoospermia are the leading causes of male infertility in these regions. In addition to this, it also shows empirically that semen parameters decrease with advancement in age. These findings are the first report on male-factor infertility prevalence in Sub-Saharan countries, necessitating a thorough investigation on the underlying factors promoting this worrisome decline.
LINC00937 acts bi-function in oral squamous cell carcinoma cell growth and immune cell infiltration regulated FGR/IL10RA axis
Authors: LI Yue-Ying , Liu Dong Xu , ZANG Xi-Yan , LU Jia-Yi , WU Ke-Han , Ma Chen-Hao , LI Jin-Liang , WU Zi-Han , ZHAO Shu-Ning , WANG He , SUN Hui , GAO Li 
Published: January 2023

Abstract
LINC00937 is a long non-coding RNA (lncRNA) up-regulated in multiple human carcinomas, such as oral squamous cell carcinoma (OSCC). However, the significance and potential mechanisms of LINC00937 in OSCC progression are still primarily need discussed. In our study, we first showed the part of LINC00937 in OSCC progress, and our data showed that LINC00937 has up-regulated in The Cancer Genome Atlas (TCGA) datasets and OSCC tissues. At the same time, LINC00937 is located in the cell nucleus. Up-regulate level of LINC00937 was correct with advanced clinical survival as well as HPV expression. Receiver operating curve (ROC) results showed the diagnostic ability of LINC00937 (AUC = 0.712) in OSCC patients. Meanwhile, LINC00937 is positively correct with FGR and IL10RA expression, downstream genes for LINC00937 in OSCC progress by PPI. In addition, pathway analysis showed LINC00937 was correlated with immune infiltrated cells relative-singling pathways. Finally, the silence of LINC00937 suppresses OSCC cell growth, induces apoptosis, inhibits migration, and invades in vitro. Our findings demonstrated that LINC00937 regulates expression of the downstream gene (FGR/IL10RA) and then regulates cell growth as well as metastasis in OSCC. Our data proved a piece of evidence that LINC00937 could serve as a prognostic biomarker for OSCC controlling immune infiltration.
Altered IGF2 imprint leads to accelerated adipogenesis and early onset of metabolic syndrome in mice following prenatal arsenic exposure
Authors: Koshta Kavita , Chauhan Anchal , Singh Sukhveer , Gaikwad Anil Nilkanth , Kumar Mahadeo , Srivast Vikas 
Published: January 2023

Abstract
Developmental exposure to environmental pollutants has been shown to promote adverse health outcomes in offspring. Exposure to heavy metals such as arsenic which also has endocrine disrupting activity is being increasingly linked with cancers, diabetes and lately with the metabolic syndrome (MetS). In this work we have assessed the effects of only prenatal arsenic exposure on developmental programming of MetS in offspring. In our study, only prenatal arsenic exposure led to reduced birth weight which was followed by catch-up growth, adiposity, elevated serum triglycerides levels and hyperglycemia in male offspring. Significant adipocytes dysfunction was observed in offspring with increased hypertrophy, insulin resistance, and chronic inflammation in epididymal white adipose tissue. Adipose tissue regulates the metabolic health of individual and its dysfunction resulted in elevated serum levels of metabolism regulating adipokines (Leptin, Resistin) and pro-inflammatory cytokines (PAI-1, TNFα). The progenitor adipose derived stem/stromal cells (AdSCs) from exposed progeny had increased proliferation and adipogenic potential with increased lipid accumulation. We also found increased activation of Akt, ERK1/2 & p38 MAPK molecules in arsenic exposed AdSCs along with increased levels of phospho-Insulin-like growth factor-1 receptor and its upstream activator Insulin-like growth factor-2 (IGF2). Overexpression of IGF2 was found to be due to arsenic mediated DNA hypermethylation at ICR region located − 2kb to -4.4kb upstream of H19 Transcription start site (TSS) which caused reduction in the conserved zinc finger protein (CTCF) occupancy. This further led to persistent activation of AKT & MAPK signaling cascade and enhanced adipogenesis leading to early onset of metabolic syndrome in the offspring.
Unilateral levator avulsion increases the risk of de novo stress urinary incontinence after cystocele repair surgery
Authors: HU Pan , LIU Lubin , WEI Linna , WANG Ying , LEI Li 
Published: January 2023

Abstract
Purpose To estimate whether levator ani injuries increase the risk of de novo urinary stress incontinence after cystocele repairing surgery.
Methods A total of 1124 women who were underwent cystocele repaired surgeries were recruited and assessed for eligibility from January,1,2012 to April,30,2021. All data related to patients and surgeries was collected and followed-up, which included general conditions, clinical examination, ultrasound results and follow-up results. Data was compared according to the follow up results. The relative risk (RR) of de novo urinary stress incontinence (SUI) with levator avulsion was calculated.
Results 336 women were involved eventually. They were divided into no complication group (n=249), de novo SUI group (n=68) and other complications group (n=19). It seemed elder or obese women were more likely to get poor results after TVM repair (p＜0.05). In de novo SUI group, incidence of levator avulsion before surgery were higher than the other two groups(p=0.001). TVM can significantly change a prolapse to point Aa and Ba on POP-Q (p＜0.05). RR ratios of de novo SUI with unilateral avulsion group is 2.60 (95%CI 1.39-4.87), and 2.58(95%CI 0.82 -8.15) for bilateral group.
Conclusion Unilateral levator avulsion is a risk factor of de novo SUI after cystocele repair surgery.
Fruit Residues as Biomass for Bioethanol Production Using Enzymatic Hydrolysis as Pretreatment
Authors: Favaretto Danúbia Paula Cadore , Rempel Alan , Lanzini Julia Roberta , Silva Ana Carolina Mattana , Lazzari Tauane , Barbizan Luiza Desengrini , Brião Vandré Barbosa , Colla Luciane Maria , Treichel Helen 
Published: January 2023

Abstract
The commercialization of fruits in the market generates a large amount of waste because they are perishable and have a short shelf life; thus, they are discarded. This study aimed to provide a noble end to discarded fruits that have fermentable sugars. These fruits were collected from supermarkets in the city of Passo Fundo, Brazil, and underwent an enzymatic hydrolysis process. The ability of four pectinases, two amylases, one xylanase and one cellulase to release reducing sugars from fruit biomass before fermentation with two yeast strains (S. cerevisiae CAT-1 and S. cerevisiae Angel) for bioethanol production was investigated, obtaining a total of RS (Reducing sugar) of 359.38 mg/L. A fermentation with yeast S. cerevisiae CAT-1 resulted in 98% consumption of RS and the production of a total of 28.02 g/L of ethanol. Furthermore, fermentation with the yeast S. cerevisiae Angel, resulted in 97% RS consumption and 31.87 g/L ethanol production, which was the best result obtained throughout all the tests of hydrolysis.
Radical polymerisation synthesis of novel poly(propranolol) acrylate and methacrylate polymers possess antibacterial activity and metal ion grabbing ability
Authors: Kumar S. John Santhosh , Kamaraj P. , Vivekandan P. A. , Periyasami Govindasami , Rahaman Mostafizur , Karthikeyan Perumal 
Published: January 2023

Abstract
The authors have requested that this preprint be removed from Research Square.