bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial transcriptome reveals disturbance of peri-infundibular immune infiltration in androgenetic alopecia
Authors: Charoensuksira Sasin , Tantiwong Supasit , Pongklaokam Juthapa , Hanvivattanakul Sirashat , Surinlert Piyaporn , Krajarng Aungkana , Thanasarnaksorn Wilai , Hongeng Suradej , Ponnikorn Saranyoo 
Published: June 2023

Abstract
Peri-infundibular immune infiltration (PII) is a crucial cluster of immune cells associated with human hair follicles (HFs), with potential importance in HF-immune interactions. Androgenetic alopecia (AGA) is characterized by microinflammation and abnormal immune responses, especially in the PII region. However, the precise patterns of immune dysregulation remain unclear due to limitations in current analysis techniques preserving tissue architecture. Here, we utilized spatial transcriptome profiling, a high-throughput analysis technology, to investigate the immunological disturbances within the PII region. We evaluated overall immune infiltrates, conducted gene set enrichment analysis (GSEA), cellular deconvolution, differential expression analysis, over-representation analysis, protein-protein interaction (PPI) network, and upstream regulator analysis to identify cell types and molecular dysregulation in immune cells. Our results demonstrated significant differences in immune signature between the PII of AGA patients (PII-A) and the PII of control donors (PII-C). PII-A exhibited enrichment in CD4 + helper T cells, distinct immune response patterns, and a bias toward the T helper (Th) 2 response. Immunohistochemistry confirmed the disturbed T cell subpopulations, with increased CD4 + T cells displaying elevated Th2 response but reduced Th1-cytotoxic response compared to PII-C. These findings reveal the unique immune landscapes of PII-A and PII-C, suggesting possibilities for the development of innovative treatment approaches.
SNAPSJIA study
Survey of Adolescents' Needs and Parents' Views on Sexual Health in Juvenile Idiopathic Arthritis
Authors: SAVEL Carine , CHAUSSET Aurélie , BERLAND Pauline , GUIGUET-AUCLAIR Candy , CABANE Laura , FAUTREL Bruno , GAUDIN Philippe , GUILLOT Pascale , HAYEM Gilles , LAFARGE Delphine , MERLIN Etienne , PEZIERE Nadine , SORDET Christelle , TROPE Sonia , TOURNADRE Anne , MALOCHET Sandrine , COHEN Jean David 
Published: June 2023

Abstract
Background: Although the advent of new therapeutics for juvenile idiopathic arthritis (JIA) patients has considerably lessened the impact of the disease and reduced sequelae, the outcomes of JIA remain important in their lives and more specifically for sex life. According to the world health organization, sexual health (SH) is "a state of physical, emotional, mental and social well-being in relation to sexuality". The aim of the study was to determine the expectations of adolescents with JIA and the perceptions of their parents regarding knowledge and communication with healthcare providers (HCPs) in the field of SH.
Methods: From September 2021 to April 2022, a French survey with anonymous self-administered questionnaires, was conducted among JIA patients and their parents in nine rheumatology centers and three patient associations. On the advice of a psychiatrist, we interviewed adults (age 18–45 years) for necessary hindsight from the subject.
Results: 76 patient and 43 parent questionnaires were analyzed. Half the patients thought JIA impacted their love life, the results were less clearcut for their sex life. 58.7% would be comfortable discussing the subject with HCPs, but only 26.3% had done so, mainly regarding biomedical issues. Patients and parents thought the topic should ideally be addressed in hospital in an individual patient education session (51.3%, 34.9% respectively), a regular consultation (47.4%, 53.5%) or a dedicated consultation requested by the adolescent without parents being informed (38.2%, 20.9%). Most thought HCPs should be proactive (77.6% of patients, 69.8% of parents). The use of digital resources was significantly more cited by patients than by parents: video information (29.0% vs. 9.3%, p= 0.0127) smartphone application (25.0% vs. 9.3%, p = 0.0372).
Conclusion: HCPs should address this unmet need for SH in hospital. The main difference between patients and parents was the use of digital tools, a preferred vector of communication for adolescents. Parents need to be reassured about their content.
Clinical trial registration number: NCT04791189
Whole genome sequence of Citrus yellow vein clearing virus CA1 isolate
Authors: Sun Yongduo , HelmRodriguez Sydney , Yokomi Raymond 
Published: June 2023

Abstract
Objectives:
Citrus yellow vein clearing virus (CYVCV) is an emerging disease that poses a significant threat to the citrus industry in California. In this study, the viral genomic RNA was isolated from Eureka lemon plants in the greenhouse exhibiting CYVCV symptoms. Subsequently, the corresponding DNA genome amplicon was sequenced and annotated. These efforts expand the genotype database of CYVCV, which aims to enhance detection assays, promote understanding of the virus's genetics and evolution, and support the management of this disease.
Data description:
In this report, we present the complete genome sequence of the CYVCV California isolate (CA1). The genome was found to be 7,530 bp in length, with a G+C content of 51.7%. The 5′ and 3′ termini were determined using 5′ and 3′ termini rapid amplification of cDNA ends (RACE) systems. Furthermore, our analysis revealed the presence of six open reading frames (ORFs) potentially encoding proteins. All sequence data and annotation have been deposited in GenBank under the accession number OR037276.
Inhibition and eradication effects of Thymus leptobotrys and Thymus satureioïdesessential oils against multidrug resistant Acinetobacter baumannii biofilms
Authors: Laktib Asma , Fayzi Lahbib , Megdar Soufiane El , Kheloui Raja El , Msanda Fouad , Cherifi Khalil , Hassi Mohammed , alla Aicha Ait , Hamadi Fatima 
Published: June 2023

Abstract

In the present study, the antibacterial activity of Thymus leptobotrys and Thymus satureioïdes essential oils was evaluated against nine multidrug resistant Acinetobacter baumannii strains. Besides, their inhibition and eradication effects were investigated, for the first time, against the biofilm form of the tested multidrug resistant bacteria. Gas chromatography–mass spectrometry analysis of the targeted essential oils highlighted the predominance of carvacrol and borneol, respectively. Their antibacterial activity test revealed considerable inhibition zones’ diameters (p < 0.05), ranging from 35 ± 1.4 to 43.6 ± 0.5 mm (Thymus leptobotrys) and from 16.6 ± 1.5 to 22 ± 2.8 mm (Thymus satureioïdes). Very low minimal inhibitory and bactericidal concentrations (p < 0.05) were also noted, varying from 0.78 to 12.5 mg/mL. Four strains out of nine (AB 6, AB 7, AB 8, AB 9) considered as the strongest biofilm producers by congo red agar and crystal violet methods, were chosen for the evaluation of biofilm inhibition and eradication effects of Thymus leptobotrys and Thymus satureioïdes essential oils. Great biofilm inhibition percentages were noted with 90.20% (Thymus satureioïdes) and 97.27% (Thymus leptobotrys) as the highest rates. Forty-eight hours biofilms were more resistant to the essential oils’ action. Low eradication proportions were observed ranging from 0–49%. Thus, Thymus leptobotrys and Thymus satureioïdes essential oils could be considered as a natural source of bioactive molecules and used as an alternative approach to prevent biofilm formation of multidrug resistant Acinetobacter baumannii.
Checklist of the Eunicidae Berthold, 1827 (Annelida) for the Iberian Peninsula (NE Atlantic)
Authors: Besteiro Celia , Parapar Julio , Moreira Juan 
Published: June 2023

Abstract

A checklist of the eunicids (Annelida: Eunicidae) reported in the Iberian Peninsula is presented based on published records. To date, 28 species have been reported belonging to 7 genera, being the most diverse Eunice Cuvier, 1817. Data is provided for each species regarding sampling locations, habitat, and depth.
IKZF1 rs4132601 and rs11978267 Gene Polymorphisms and Juvenile Systemic Lupus Erythematosus; relation to Lupus Nephritis
Authors: Mosaad Youssef M. , Hammad Ayman , Shouma Amany , Darwish Mohamed , Hammad Enas M. , Sallam Rehab AR. , ELTantawi Noha T. , Abdel-Azeem Heba A. , Youssef Laila F. , El-Khier Noha T. Abou , Fawzy Iman M. , Alwasify Mona 
Published: June 2023

Abstract

Background: The demographic factors, the socioeconomic status and the ethnicity of populations are important players that determine the incidence, the prevalence, and the spectrum of systemic lupus erythematosus (SLE) clinical presentations in different populations. Therefore, the purpose of the present research was to search for the presence of a relation between the IKZF1 rs4132601 and rs11978267 single nucleotide polymorphisms (SNPs) and the development of pediatric SLE (pSLE) and also to search for the association with the clinical presentations including lupus nephritis (LN).
Methods: After DNA extraction from EDTA blood samples for 104 pSLE patients and 286 healthy controls, the investigated SNPs (IKZF1 rs4132601 and rs11978267) were genotyped using TaqMan-Real-time PCR.
Results: The G allele and GG homozygous genotype of IKZF1 rs4132601 were associated with pSLE (p‹0.001, OR 2.97 and p‹0.001, OR 3.2 respectively). The GG and GA haplotype were more frequent in pSLE patients than other haplotypes (p‹0.001, OR 3.47, and p=0.001, OR=2.8 respectively). The studied SNPs have no impact on the different features of pSLE. The rs4132601 TG genotype was significantly associated with proliferative LN and class IV of LN biopsy (p=0.01, and p=0.029 respectively)
Conclusion: The IKZF1 rs4132601 can be considered a risk factor for SLE in the cohort of Egyptian children. The TG and GG genotypes of the IKZF1rs4132601 may predispose to proliferative LN.
A Novel Synchronized Stimulation Method to Improve the Tactile Localization Ability of Post-Stroke Patients
Authors: Rostami Mohammad , Barzegar Mojtaba , Ahmadi Maryam , Mehrpour Masoud , Nasimi Zahra , Attari Fatemeh , Saeedi Hamid , Bahmani Zahra 
Published: June 2023

Abstract
Background
Transcranial direct-current stimulation (tDCS) has shown promise in enhancing neural plasticity and functional abilities in post-stroke patients. However, determining the optimal protocol for this method remains an open question. Our study proposes a novel approach: synchronized stimulation that combines mechanical and electrical stimuli. We hypothesize that this approach will enhance tactile localization ability in post-stroke patients.
Methods
We recruited a total of 23 patients and conducted four different types of experiments involving periodic mechanical stimulation on their fingertips. The primary objective was to assess the participants' ability to accurately localize the location of the mechanical stimulation. In one experiment, only mechanical stimulation was administered. In the remaining three experiments, electrical stimulations were combined with the mechanical stimulation. The electrical stimulations comprised of one of the following protocols: (1) tDCS pulses administered solely for the initial five seconds of the session, (2) continuous tDCS pulses throughout the entire duration of the mechanical stimulation, and (3) tDCS pulses synchronized precisely with the timing of the mechanical stimulation.
Results
A noteworthy enhancement in tactile localization ability was observed when the electrical and mechanical stimulations were synchronized.
Conclusion
Our findings demonstrate that the integration of electrical brain stimulations with simultaneous mechanical stimulation of the fingertips resulted in enhanced neural activities. This synchronized integration holds the potential to improve perception and may serve as a vital approach in the treatment of post-stroke patients.
Identification and immuno-infiltration analysis of cuproptosis regulators in human atherosclerosis
Authors: Ming Shaopeng , Wen Haiming , Zeng Chunrong , Li Zhaoyu , Wang Chaona , Yan Shu , Liu Hongtao 
Published: June 2023

Abstract
Introduction
The development of atherosclerosis (AS) may be aided by cuproptosis. As a result, we examined the cuproptosis regulators in human AS, gauged the degree of immune cell infiltration, and developed a prediction model.
Methods
We obtained the GSE100927 gene expression dataset associated with AS from the Gene Expression Omnibus (GEO) database and used it to identify cuproptosis-related differentially expressed genes (CuDEGs). This was accomplished by comparing AS samples and control samples. We also examined the relationship between CuDEGs and immune cell infiltration status, and investigated the molecular groupings of both CuDEGs and immune cell infiltration status. To pinpoint cluster-specific differentially expressed genes, we employed weighted gene co-expression network analysis (WGCNA). Furthermore, gene set variation analysis (GSVA) was carried out to annotate the enriched genes. From four different machine-learning models, we selected the model with the best performance. Lastly, we validated the accuracy of our predictions using nomograms and ROC curves.
Results
Our study confirmed the presence of CuDEGs and activated immune responses among AS and control samples. We identified 12 CuDEGs through the dataset, and we also discovered two clusters in AS. Analysis of immune cell infiltration showed that there is heterogeneity in immunity between these two clusters. Cuproptosis-related molecular Cluster 2 was marked by enhanced expressions of NLRP3, SLC31A1, FDX1, LIPT2 and CDKN2A. And Cluster 1 exhibited a higher proportion of T cells CD4 memory resting、Monocytes、Macrophages M1 and Mast cells resting. And enriched KEGG pathways revealed the pathway of leukocyte transendothelial migration was up-regulated in Cluster 1. We subsequently developed a support vector machine (SVM) model based on five genes, which demonstrated good performance in predicting AS in the external validation dataset (AUC = 0.895). Our results indicate that this combined nomogram is highly accurate in predicting AS.
Conclusion
Our study sheds light on the relationship between AS and cuproptosis, as well as the association between CRGs and immune-infiltrated cells in AS. Additionally, we have established a good predictive model.
The impacts of a combination of Al2O3 nanoparticles with glass powder and fly ash in concrete
Authors: chingkheinganba telem , Lallotra Balwinder 
Published: June 2023

Abstract
One of the things that emits carbon dioxide besides deforestation and the use of petroleum derivatives is the business of making cement. It's an intriguing potential to use glass in concrete to save waste disposal costs. Glass is a non-toxic substance that may be used and recycled repeatedly without making any chemical composition changes. When the glass particles are smaller than 75 m, it has a high silica content, which makes it potentially pozzolanic. The chemical reaction taking place in the concrete's pore solution between the silica-rich glass particles and the alkali, known as the Alkali-Silicate reaction, is a major worry when using glass in concrete. If proper measures aren't taken to lessen its effects, concrete's stability may be severely damaged by the reaction. Fly ash is used in glass concrete to improve workability and durability, as well as to lessen the alkali silica reaction and create a more environmentally friendly concrete mix. Comparing the compressive strength of the concrete with or without nano-Al2O3 particles, the concrete with nano-Al2O3 particles had a significantly higher compressive strength. Therefore, continuous research is conducted to improve the characteristics of concrete. The current paper inspects the effect of a mix of Al2O3 nanoparticles along with fly ash and glass powder on mechanical properties( compressive, flexural, split tensile) strength, microscopic study by using SEM and non destructive test using UPV. As a partial substitution of Ordinary Portland cement, nano alumina is used at 0.5%, 1%, 1.5%, and 2%. Glass powder substituted sand at a fixed proportion of 15% and fly ash substituted cement at a fixed proportion of 15%. Using a scanning electron microscope, the microstructure and surface morphology of modified cement concretes were evaluated (SEM). The results concluded that at 1.5% substitution of nano Al2O3, it gets ultimate strength.
Research on the causal mechanism of a rock-burst accident in a longwall roadway and its prevention measures
Authors: Chen Ying , Zhang Zikai , Cao Chen , Bao Shiji , Wang Shuai , Xu Guangyuan 
Published: June 2023

Abstract
Based on the characteristics of the disaster and the geo-conditions at the scene, this study analyzed the occurrence mechanism of a serious rock-burst accident occurred in Tangshan Coal Mine, China. Ground stress measurement shows that the mine is in high ground stress area dominated by horizontal tectonic stress. Laboratory testing showed that the coal was a hard seam over-bedded by a thick and hard roof stratum. The calculation results showed that the accident occurred in the roof rebounding area. It is proposed that the hard roof and the hard coal seam formed a seesaw structure around the working face. The vertical pressure relief caused the rib coal mass losing its clamping forces from the roof and floor, rushing into the roadway, resulting in rock-burst accident. Based on the causality mechanism of the rock-burst disaster developed in this study, pertinent coal bump prevention measures have been undertaken in practice. Large-diameter boreholes were drilled to eliminate the pivot effect of the seam. Roof blasting was undertaken preventing the roof from forming a seesaw plank. This study developed a new causality mechanism of rock burst for coal mines with hard roof and hard seam geo-conditions.
Analysis of a macrophage carbamylated proteome reveals a function in post-translational modification crosstalk
Authors: You Youngki , Tsai Chia-Feng , Patel Rishi , Sarkar Soumyadeep , Clair Geremy , Zhou Mowei , Liu Tao , Metz Thomas O. , Das Chittaranjan , Nakayasu Ernesto S. 
Published: June 2023

Abstract
Background.
Lysine carbamylation is a biomarker of rheumatoid arthritis and kidney diseases. However, its cellular function is understudied due to the lack of tools for systematic analysis of this post-translational modification (PTM).
Methods.
We adapted a method to analyze carbamylated peptides by co-affinity purification with acetylated peptides based on the cross-reactivity of anti-acetyllysine antibodies. We integrated this method into a mass spectrometry-based multi-PTM pipeline to simultaneously analyze carbamylated and acetylated peptides in addition to phosphopeptides were enriched by sequential immobilized-metal affinity chromatography.
Results.
By testing the pipeline with RAW 264.7 macrophages treated with bacterial lipopolysaccharide, 7,299, 8,923 and 47,637 acetylated, carbamylated, and phosphorylated peptides were identified, respectively. Our analysis showed that carbamylation occurs on proteins from a variety of functions on sites with similar as well as distinct motifs compared to acetylation. To investigate possible PTM crosstalk, we integrated the carbamylation data with acetylation and phosphorylation data, leading to the identification 1,183 proteins that were modified by all 3 PTMs. Among these proteins, 54 had all 3 PTMs regulated by lipopolysaccharide and were enriched in immune signaling pathways, and in particular, the ubiquitin-proteasome pathway. We found that carbamylation of linear diubiquitin blocks the activity of the anti-inflammatory deubiquitinase OTULIN.
Conclusions
Overall, our data show that anti-acetyllysine antibodies can be used for effective enrichment of carbamylated peptides. Moreover, carbamylation may play a role in PTM crosstalk with acetylation and phosphorylation, and that it is involved in regulating ubiquitination in vitro.
Oral Ulcers as A Manifestation Of COVID-19 Infection: A Cross-Sectional Study in Egypt
Authors: Mohamed Sally Ibrahim , Shabaan Alshaimaa Ahmed , Mahrous Aliaa Ibrahim , Ahmed Basma Abdelrahman , Aboulmagd Inass Abdelrahaman , Radwan Mohamed Mostafa , Hafed Layla , Baz Safaa , Blunt Tadhg , Elkoumi Ahmed , Amer Hatem 
Published: June 2023

Abstract
Objectives
This study aimed to examine the prevalence of oral ulcers in COVID-19 patients within the Egyptian population, while considering potential risk factors, assessing pain intensity, and exploring the implications for early detection and treatment.
Materials and Methods
A questionnaire was developed consisting of 16 questions that included both open-ended and close-ended formats. The survey gathered information on the patient’s demographic characteristics, medical background, oral hygiene practices, associated symptoms, and treatments received.
Results
Of 363 participants with COVID-19, oral ulcers were found in 34.1% of cases, predominantly affecting the tongue (25.8%), gums (11.3%), and inner oral mucous membrane (9.7%). Ulcer development was significantly associated with corticosteroid therapy, abdominal pain, shortness of breath, nasal congestion, and diarrhea (p < 0.05). However, there were no significant associations with age, gender, brushing habits, or co-morbidities. Pain intensity increased, as assessed on the pain scale, by 2.08 with abdominal pain and by 0.06 with age, while it decreased with good brushing habits by 2.1 and with patients in the ICU by 4.4.
Conclusions
The survey findings revealed that tongue ulcers were the most commonly observed oral manifestation among COVID-19-infected individuals in Egypt. The occurrence of ulcers was associated with factors such as corticosteroid therapy, abdominal pain, nasal congestion, and diarrhea. The severity of pain experienced was influenced by abdominal pain and age.
Clinical Relevance
Recognizing the clinical importance of oral ulcers in COVID-19 aids in the identification of the tangible discomfort experienced by patients and the potential implications associated with these ulcers as an indicative sign of COVID-19 infection.
Hydrostatic support and ultrasound-assisted SPIF error and process parameter optimization study
Authors: Jing Zhangshuai , Zheng Jianming , Yang Mingshun , Li Yan , Peng Chao , Zhao Xingbai 
Published: June 2023

Abstract

To solve the problems of overhanging back of the part and difficult forming and to improve the accuracy of single-point incrementally formed parts, considering many error influencing factors, this paper introduces hydrostatic support and ultrasonic vibration-assisted technology into single-point incremental forming and investigates the influence of process parameters on the geometric shape error of formed parts under the conditions of hydrostatic support and ultrasonic vibration, taking the conical table part as the object. A four-factor, three-level process experiment with static pressure, amplitude, frequency, and tool head diameter as variables was designed using Box-Behnken Design, modeled by the least squares method in the response surface method, and optimized by the satisfaction function. The results show that the static pressure support and ultrasonic vibration can effectively reduce the error of the part, and the error is minimized when the static pressure value is 0.058 MPa, the amplitude is 0.019 mm, the vibration frequency is 25 KHz, and the tool head diameter is 15.7 mm. The method and conclusions presented in this paper can provide a reference for the study of single-point increments of hydrostatic support-ultrasonic vibration.