bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual-stimuli responsive smart nanoprobe for precise diagnosis and synergistic multi-modalities therapy of superficial squamous cell carcinoma
Authors: Zhang Peisen , Cui Yingying , Wang Jian , Cheng Junwei , Zhu Lichong , Liu Chuang , Yue Saisai , Pang Runxin , Guan Jiaoqiong , Xie Bixia , Zhang Ni , Qin Meng , Jing Lihong , Hou Yi , Lan Yue 
Published: January 2023

AbstractBackgroundAlthough the promising advancements of current therapeutic approaches is available for the squamous cell carcinoma (SCC) patients, the clinical treatment of SCC still faces many difficulties. The surgical irreparable disfigurement and the postoperative wound infection largely hamper the recovery, and the chemo/radiotherapy leads to toxic side effects.ResultsHerein, a novel pH/Hyaluronidase (HAase) dual-stimuli triggered smart nanoprobe FeIIITA@HA has been designed through the biomineralization of Fe3+and polyphenol tannic acid (TA) under the control of hyaluronic acid (HA) matrix. With the HA residues on the outer surface, FeIIITA@HA nanoprobes can specifically target the SCC cells through the over-expressed CD44, and accumulate in the carcinoma region after intravenously administration. The abundant HAase in carcinoma microenvironment will trigger the degradation of HA molecules, thereby exposing the FeIIITA complex. After ingesting by tumor cellsviaCD44 mediated endocytosis, the acidic lysosomal condition will further trigger the protonation of TA molecules, finally leading to the Fe3+release of nanoprobe, and inducing a hybrid ferroptosis/apoptosis of tumor cells through peroxidase activity and glutathione depletion. In addition, Owing to the outstandingT1magnetic resonance imaging (MRI) performance and phototermal conversion efficiency of nanoprobes, the MRI-guided photothermal therapy (PTT) can be also combined to complement the Fe3+-induced cancer therapy. Meanwhile, it was also found that the nanoprobes can promote the recruitment of CD4+and CD8+T cells to inhibit the tumor growth through the cytokines secretion. In addition, the FeIIITA@HA nanoprobes can be eliminated from the body and no obvious adverse side effect can be found in histological analysis, which confirmed the biosafety of them.ConclusionThe current FeIIITA@HA nanoprobe has huge potential in clinical translation in the field of precise diagnosis and intelligent synergistic therapy of superficial SCC. This strategy will promisingly avoid the surgical defects, and reduce the systemic side effect of traditional chemotherapy, paving a new way for the future SCC treatment.Graphical Abstract
Total coumarins of Pileostegia tomentella induces cell death in SCLC by reprogramming metabolic patterns, possibly through attenuating β-catenin/AMPK/SIRT1
Authors: Liu Ying , Wu Kun , Li Li , Zhu Fucui , Wang Li , Su Hua , Li Ying , Lu Lu , Lu Guoshou , Hu Xiaoxi 
Published: January 2023

Abstract
Background
Small-cell lung cancer (SCLC) is a high malignant and high energy-consuming type of lung cancer. Total coumarins of Pileostegia tomentella (TCPT) from a traditional folk medicine of Yao minority, is a potential anti-cancer mixture against SCLC, but the pharmacological and molecular mechanism of TCPT remains largely unknown.

Methods
Screening of viability inhibition of TCPT among 7 cell lines were conducted by using CCK-8 assays. Anti-proliferative activities of TCPT in SCLC were observed by using colony formation and flow cytometry assays. Morphological changes were observed by transmission electron microscope and Mito-Tracker staining. High Throughput RNA-seq analysis and bio-informatics analysis were applied to find potential targeted biological and signaling pathways affected by TCPT. The mRNA expression of DEGs and protein expression of signalling proteins and metabolic enzymes were verified by qPCR and Western blot assays. Activity of rate-limiting enzymes and metabolite level were detected by corresponding enzyme activity and metabolites kits. Xenograft nude mice model of SCLC was established to observe the in vivo inhibition, metabolism reprogramming and mechanism of TCPT.

Results
TCPT treatment shows the best inhibition in SCLC cell line H1688 rather than other 5 lung cancer cell lines. Ultrastructural investigation indicates TCPT induces mitochondria damage such as cytoplasm shrinkage, ridges concentration and early sight of autolysosome, as well as decrease of membrane potential. Results of RNA-seq combined bio-informatics analysis find out changes of metabolism progression affected the most by TCPT in SCLC cells, and these changes might be regulated by β-catenin/AMPK/SIRT1 axis. TCPT might mainly decline the activity and expression of rate-limiting enzymes, OGDH, PDHE1, and LDHA/B to reprogram aerobic oxidation pattern, resulting in reduction of ATP production in SCLC cells. Xenograft nude mice model demonstrates TCPT could induce cell death and inhibit growth in vivo. Assimilate to the results of in vitro model, TCPT reprograms metabolism by decreasing the activity and expression of rate-limiting enzymes (OGDH, PDHE1, and LDHA/B), and attenuates the expression of β-catenin, p-β-catenin, AMPK and SIRT1 accordance with in vitro data.

Conclusion
Our results demonstrated TCPT induces cell death of SCLC by reprograming metabolic patterns, possibly through attenuating master metabolic pathway axis β-catenin/AMPK/SIRT1.
Role of GRPR in Acupuncture Intervention in the “Itch-scratch Vicious Cycle” Spinal Circuit of Chronic Pruritus
Authors: Liu Jia-jia , Li Xuemei , Guo Jing , Yu Shuguang , Yang Sha 
Published: January 2023

AbstractMany previous studies have shown the potential antipruritic effect of acupuncture. This paper reviews the antipruritic mechanisms of acupuncture according to these aspects: sample characteristics, detail of intervention, and effects evaluation. The majority of research on acupuncture’s antipruritic effect has focused on primary afferents of the peripheral mechanism. Relatively few studies, however, have addressed the central mechanisms. Combination the latest research achievements of chronic itch, gastrin-releasing peptide receptor (GRPR) in the dorsal horn of the spinal cord may represent the first molecule identified that is dedicated to mediating the itch response and may provide an important therapeutic target for the treatment of chronic pruritic conditions. Therefore, GRPR may be a new target for acupuncture to relieve itch in the future and provide new ideas for acupuncture intervention in the mechanisms of the spinal level of the “itch-scratch vicious cycle” of chronic itch.
Two-center randomized controlled trial comparing oral chloral hydrate and intranasal combination of dexmedetomidine and ketamine for procedural sedation in children: study protocol
Authors: Jang Young-Eun , Joo Eun-Young , Lee Ji-Hyun , Kim Eun-Hee , Kang Pyoyoon , Park Jung-Bin , Kim Hee-Soo , Kim Jin-Tae 
Published: January 2023

Abstract
Background
Oral chloral hydrate is widely used in pediatric sedation. Intranasal dexmedetomidine has been increasingly used for pediatric sedation; however, its improvement is warranted. The combination of dexmedetomidine with ketamine can improve onset and hemodynamic stability while maintaining sedative efficacy. This study aims to determine the efficacy and safety of intranasal combination of dexmedetomidine and ketamine compared to oral chloral hydrate.

Methods
This is a prospective, parallel-arm, single-blinded, two-center, superiority randomized controlled trial with 1:1 allocation, designed to compare the effects of intranasal combination of dexmedetomidine and ketamine with those of oral chloral hydrate. We shall enroll 136 patients aged < 7 years old in this study. Prior to the procedure, we shall randomize each patient into the control group (oral chloral hydrate 50 mg/kg) or study group (intranasal dexmedetomidine 2 μg/kg and ketamine 3 mg/kg). The primary outcome will be the rate of achieving an adequate sedation level (6-point Pediatric Sedation State Scale 1, 2, or 3) within 15 min. In addition, we shall measure the sedation time, sedation failure rate, completion of procedure, adverse events, patient acceptance, and physician satisfaction.

Discussion
This study will provide evidence of the efficacy and safety of the intranasal combination of dexmedetomidine and ketamine in comparison with oral chloral hydrate.

Trial registration
ClinicalTrials.gov, NCT04820205. Registered on 19th March 2021
An intermixed method for solving the combination of mixed variational inequality problems and fixed-point problems
Authors: Khuangsatung Wongvisarut , Kangtunyakarn Atid 
Published: January 2023

AbstractIn this paper, we introduce an intermixed algorithm with viscosity technique for finding a common solution of the combination of mixed variational inequality problems and the fixed-point problem of a nonexpansive mapping in a real Hilbert space. Moreover, we propose the mathematical tools related to the combination of mixed variational inequality problems in the second section of this paper. Utilizing our mathematical tools, a strong convergence theorem is established for the proposed algorithm. Furthermore, we establish additional conclusions concerning the split-feasibility problem and the constrained convex-minimization problem utilizing our main result. Finally, we provide numerical experiments to illustrate the convergence behavior of our proposed algorithm.
Low-power multi-cloud deployment of large distributed service applications with response-time constraints
Authors: Singh Babneet , Kaur Ravneet , Woodside Murray , Chinneck John W. 
Published: January 2023

AbstractDistributed service applications make heavy use of clouds and multi-clouds, and must (i) meet service quality goals (e.g. response time) while (ii) satisfying cloud resource constraints and (iii) conserving power. Deployment algorithms must (iv) provide a solution meeting these requirements within a short time to be useful in practice. Very few existing deployment methods address the first three requirements, and those that do take too long to find a deployment. The Low-Power Multi-Cloud Application Deployment (LPD) algorithm fills this gap with a low-complexity heuristic combination of generalized graph partitioning between clouds, bin-packing within each cloud and queueing approximations to control the response time. LPD has no known competitor that quickly finds a solution that satisfies response time bounds. A host execution time approximation for contention is fundamental to achieving sufficient solution speed. LPD is intended for use by cloud managers who must simultaneously manage hosts and application deployments and plan capacity to offer services such as Serverless Computing.On 104 test scenarios deploying up to 200 processes with up to 240 replicas (for scaling), LPD always produced a feasible solution within 100 s (within 20 seconds in over three-quarters of cases). Compared to the Mixed Integer Program solution by CPLEX (which took a lot longer and was sometimes not found) LPD solutions gave power consumption equal to MIP in a third of cases and within 6% of MIP in 95% of cases. In 93% of all 104 cases the power consumption is within 20% of an (unachievable) lower bound.LPD is intended as a stand-alone heuristic to meet solution time restrictions, but could easily be adapted for use as a repair mechanism in a Genetic Algorithm.
Ecological niche shifts affect the potential invasive risk of Phytolacca americana (Phytolaccaceae) in China
Authors: Xu Yifeng , Ye Xingzhuang , Yang Qianyue , Weng Huiying , Liu Yipeng , Ahmad Sagheer , Zhang Guofang , Huang Qiuliang , Zhang Tianyu , Liu Bao 
Published: January 2023

Abstract
Background
Predicting the potential habitat of Phytolacca americana, a high-risk invasive species, can help provide a scientific basis for its quarantine and control strategies. Using the optimized MaxEnt model, we applied the latest climate data, CMIP6, to predict the distribution of potential risk zones and their change patterns for P. americana under current and future (SSP126, SSP245, SSP585) climate conditions, followed by invasion potential analysis.

Results
The predictions of MaxEnt model based on R language optimization were highly accurate. A significantly high area of 0.8703 was observed for working characteristic curve (AUC value) of subject and the kappa value was 0.8074. Under the current climate conditions, the risk zones for P. americana were mainly distributed in Sichuan, Chongqing, Guizhou, Hunan, and Guangxi provinces. The contribution rate of each climatic factor of P. americana was calculated using the jackknife test. The four factors with the highest contribution rate included minimum temperature of coldest month (bio6, 51.4%), the monthly mean diurnal temperature difference (bio2, 27.9%), precipitation of the driest quarter (bio17, 4.9%), and the warmest seasonal precipitation (bio12, 4.3%).

Conclusion
Under future climatic conditions, the change in the habitat pattern of P. americana generally showed a migration toward the Yangtze River Delta region and the southeastern coastal region of China. This migration exhibited an expansion trend, highlighting the strong future invasiveness of the species. Based on the predictions, targeted prevention and control strategies for areas with significant changes in P. americana were developed. Therefore, this study emphasizes the need of an integrated approach to effectively prevent the further spread of invasive plants.
Artificial intelligence models for prediction of monthly rainfall without climatic data for meteorological stations in Ethiopia
Authors: Abebe Wondmagegn Taye , Endalie Demeke 
Published: January 2023

AbstractGlobal climate change is affecting water resources and other aspects of life in many countries. Rainfall is the most significant climate element affecting the livelihood and well-being of the majority of Ethiopians. Rainfall variability has a great impact on agricultural production, water supply, transportation, the environment, and urban planning. Because all agricultural activities and subsequent national crop production hinge on the amount and distribution of rainfall, accurate monthly and seasonal predictions of this rainfall are vital for agricultural planning. Rainfall prediction is also useful for governmental, non-governmental, and private agencies in making long-term decisions and planning in numerous areas such as farming, early warning of potential hazards, drought mitigation, disaster prevention, and insurance policy. Artificial Intelligence (AI) has been widely used in almost every area, and rainfall prediction is one of them. In this study, we attempt to investigate the use of AI-based models to predict monthly rainfall at 92 Ethiopian meteorological stations. The applicability of Artificial Neural Networks (ANNs) and Adaptive Neuro-Fuzzy Inference System (ANFIS) models in predicting long-term monthly precipitation was investigated using geographical and periodicity component (longitude, latitude, and altitude) data collected from 2011 to 2021. The experimental results reveal that the ANFIS model outperforms the ANN model in all assessment criteria across all testing stations. The Nash–Sutcliffe efficiency coefficients were 0.995 for ANFIS and 0.935 for ANN over testing stations.
A review on slow earthquakes in the Japan Trench
Authors: Nishikawa Tomoaki , Ide Satoshi , Nishimura Takuya 
Published: January 2023

AbstractSlow earthquakes are episodic slow fault slips. They form a fundamental component of interplate deformation processes, along with fast, regular earthquakes. Recent seismological and geodetic observations have revealed detailed slow earthquake activity along the Japan Trench—the subduction zone where the March 11, 2011, moment magnitude (Mw) 9.0 Tohoku-Oki earthquake occurred. In this paper, we review observational, experimental, and simulation studies on slow earthquakes along the Japan Trench and their research history. By compiling the observations of slow earthquakes (e.g., tectonic tremors, very-low-frequency earthquakes, and slow slip events) and related fault slip phenomena (e.g., small repeating earthquakes, earthquake swarms, and foreshocks of large interplate earthquakes), we present an integrated slow earthquake distribution along the Japan Trench. Slow and megathrust earthquakes are spatially complementary in distribution, and slow earthquakes sometimes trigger fast earthquakes in their vicinities. An approximately 200-km-long along-strike gap of seismic slow earthquakes (i.e., tectonic tremors and very-low-frequency earthquakes) corresponds with the huge interplate locked zone of the central Japan Trench. The Mw 9.0 Tohoku-Oki earthquake ruptured this locked zone, but the rupture terminated without propagating deep into the slow-earthquake-genic regions in the northern and southern Japan Trench. Slow earthquakes are involved in both the rupture initiation and termination processes of megathrust earthquakes in the Japan Trench. We then compared the integrated slow earthquake distribution with the crustal structure of the Japan Trench (e.g., interplate sedimentary units, subducting seamounts, petit-spot volcanoes, horst and graben structures, residual gravity, seismic velocity structure, and plate boundary reflection intensity) and described the geological environment of the slow-earthquake-genic regions (e.g., water sources, pressure–temperature conditions, and metamorphism). The integrated slow earthquake distribution enabled us to comprehensively discuss the role of slow earthquakes in the occurrence process of the Tohoku-Oki earthquake. The correspondences of the slow earthquake distribution with the crustal structure and geological environment provide insights into the slow-earthquake-genesis in the Japan Trench and imply that highly overpressured fluids are key to understanding the complex slow earthquake distribution. Furthermore, we propose that detailed monitoring of slow earthquake activity can improve the forecasts of interplate seismicity along the Japan Trench.
Comprehensive comparisons of ocular biometry: A network-based big data analysis
Authors: Yu Jinjin , Wen Daizong , Zhao Jing , Wang Yiran , Feng Ke , Wan Ting , Savini Giacomo , McAlinden Colm , Lin Xuanqiao , Niu Lingling , Chen Sisi , Gao Qingyi , Ning Rui , Jin Yili , Zhou Xingtao , Huang Jinhai 
Published: January 2023

Abstract
Purpose
To systematically compare and rank ocular measurements with optical and ultrasound biometers based on big data.

Methods
PubMed, Embase, the Cochrane Library and the US trial registry (www.ClinicalTrial.gov) were used to systematically search trials published up to October 22nd, 2020. We included comparative studies reporting the following parameters measured by at least two devices: axial length (AL), flattest meridian keratometry (Kf), steepest meridian keratometry (Ks), mean keratometry (Km), astigmatism (AST), astigmatism vectors J0 and J45, anterior chamber depth (ACD), aqueous depth (AQD), central corneal thickness (CCT), corneal diameter (CD) and lens thickness (LT). A network-based big data analysis was conducted using STATA version 13.1.

Results
Across 129 studies involving 17,181 eyes, 12 optical biometers and two ultrasound biometers (with both contact and immersion techniques) were identified. A network meta-analysis for AL and ACD measurements found that statistically significant differences existed when contact ultrasound biometry was compared with the optical biometers. There were no statistically significant differences among the four swept-source optical coherence tomography (SS-OCT) based devices (IOLMaster 700, OA-2000, Argos and ANTERION). As for Ks, Km and CD, statistically significant differences were found when the Pentacam AXL was compared with the IOLMaster and IOLMaster 500. There were statistically significant differences for CCT when the OA-2000 was compared to Pentacam AXL, IOLMaster 700, Lenstar, AL-Scan and Galilei G6.

Conclusion
For AL and ACD, contact ultrasound biometry obtains the lower values compared with optical biometers. The Pentacam AXL achieves the lowest values for keratometry and CD. The smallest value for CCT measurement is found with the OA-2000.
Morphofunctional analysis of fibroblast-like synoviocytes in human rheumatoid arthritis and mouse collagen-induced arthritis
Authors: Machado Camilla Ribeiro Lima , Dias Felipe Ferraz , Resende Gustavo Gomes , Oliveira Patrícia Gnieslaw de , Xavier Ricardo Machado , Andrade Marcus Vinicius Melo de , Kakehasi Adriana Maria 
Published: January 2023

Abstract
Background
Fibroblast-like synoviocytes (FLS) play a prominent role in rheumatoid synovitis and degradation of the extracellular matrix through the production of inflammatory cytokines and metalloproteinases (MMPs). Since animal models are frequently used for elucidating the disease mechanism and therapeutic development, it is relevant to study the ultrastructural characteristics and functional responses in human and mouse FLS. The objective of the study was to analyze ultrastructural characteristics, Interleukin-6 (IL-6) and Metalloproteinase-3 (MMP-3) production and the activation of intracellular pathways in Fibroblast like synoviocytes (FLS) cultures obtained from patients with rheumatoid arthritis (RA) and from mice with collagen-induced arthritis (CIA).

Methods
FLSs were obtained from RA patients (RA-FLSs) (n = 8) and mice with CIA (CIA-FLSs) (n = 4). Morphology was assessed by transmission and scanning electron microscopy. IL-6 and MMP-3 production was measured by ELISA, and activation of intracellular signaling pathways (NF-κB and MAPK: p-ERK1/2, p-P38 and p-JNK) was measured by Western blotting in cultures of RA-FLSs and CIA-FLSs stimulated with tumor necrosis factor-alpha (TNF-α) and IL-1β.

Results
RA-FLS and CIA-FLS cultures exhibited rich cytoplasm, rough endoplasmic reticula and prominent and well-developed Golgi complexes. Transmission electron microscopy demonstrated the presence of lamellar bodies, which are cytoplasmic structures related to surfactant production, in FLSs from both sources. Increased levels of pinocytosis and numbers of pinocytotic vesicles were observed in RA-FLSs (p < 0.05). Basal production of MMP-3 and IL-6 was present in RA-FLSs and CIA-FLSs. Regarding the production of MMP-3 and IL-6 and the activation of signaling pathways, the present study demonstrated a lower response to IL-1β by CIA-FLSs than by RA-FLSs.

Conclusion
This study provides a comprehensive understanding of the biology of RA-FLS and CIA-FLS. The differences and similarities in ultrastructural morphology and important inflammatory cytokines shown, contribute to future in vitro studies using RA-FLS and CIA-FLS, in addition, they indicate that the adoption of CIA-FLS for studies should take careful and be well designed, since they do not completely resemble human diseases.