bluuebunny/crossref_metadata_2025_split huggingface dataset:
Local YB-1, Epo, and EpoR concentrations in fractured bones: results from a porcine model of multiple trauma
Authors: Bläsius Felix Marius , Greven Johannes , Guo Weijun , Bolierakis Eftychios , He Zhizhen , Lübke Cavan , Simon Tim-Philipp , Hildebrand Frank , Horst Klemens 
Published: January 2023

AbstractLittle is known about the impact of multiple trauma (MT)-related systemic hypoxia on osseous protein concentration of the hypoxia transcriptome. To shed light on this issue, we investigated erythropoietin (Epo), erythropoietin receptor (EpoR), and Y-box binding protein 1 (YB-1) concentrations in the fracture zone in a porcine MT + traumatic hemorrhage (TH) model. Sixteen male domestic pigs were randomized into two groups: an MT + TH group and a sham group. A tibia fracture, lung contusion, and TH were induced in the MT + TH group. The total observation period was 72 h. YB-1 concentrations in bone marrow (BM) were significantly lower in the fracture zone of the MT + TH animals than in the sham animals. Significant downregulation of BM-localized EpoR concentration in both unfractured and fractured bones was observed in the MT + TH animals relative to the sham animals. In BM, Epo concentrations were higher in the fracture zone of the MT + TH animals compared with that in the sham animals. Significantly higher Epo concentrations were detected in the BM of fractured bone compared to that in cortical bone. Our results provide the first evidence that MT + TH alters hypoxia-related protein concentrations. The impacts of both the fracture and concomitant injuries on protein concentrations need to be studied in more detail to shed light on the hypoxia transcriptome in fractured and healthy bones after MT + TH.
CDCA8 induced by NF-YA promotes hepatocellular carcinoma progression by regulating the MEK/ERK pathway
Authors: Chen Erbao , He Yu , Jiang Jing , Yi Jing , Zou Zhilin , Song Qiuzi , Ren Qingqi , Lin Zewei , Lu Yi , Liu Jikui , Zhang Jian 
Published: January 2023

AbstractBackgroundHepatocellular carcinoma (HCC) is one of the most lethal malignant tumors. Cell division cycle associated 8 (CDCA8) is an important multifactorial regulator in cancers. However, its up and downstream targets and effects in HCC are still unclear.MethodsA comprehensive bioinformatics analysis was performed using The Cancer Genome Atlas dataset (TCGA) to explore novel core oncogenes. We quantified CDCA8 levels in HCC tumors using qRT-PCR. HCC cell’s proliferative, migratory, and invasive abilities were detected using a Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2′-deoxyuridine (EdU) assay, clone formation, and a Transwell assay. An orthotopic tumor model and tail vein model were constructed to determine the effects of CDCA8 inhibition in vivo. The mechanism underlying CDCA8 was investigated using RNA sequencing. The prognostic value of CDCA8 was assessed with immunohistochemical staining of the tissue microarrays.ResultsCDCA8 was identified as a novel oncogene during HCC development. The high expression of CDCA8 was an independent predictor for worse HCC outcomes both in publicly available datasets and in our cohort. We found that CDCA8 knockdown inhibited HCC cell proliferation, colony formation, and migration by suppressing the MEK/ERK pathway in vitro. Moreover, CDCA8 deficiency significantly inhibited tumorigenesis and metastasis. Next-generation sequencing and laboratory validation showed that CDCA8 silencing inhibited the expression of TPM3, NECAP2, and USP13. Furthermore, NA-YA overexpression upregulated the expression of CDCA8. CDCA8 knockdown could attenuate NF-YA-mediated cell invasion in vitro. The expression of NF-YA alone or in combined with CDCA8 were validated as significant independent risk factors for patient survival.ConclusionOur findings revealed that the expression of CDCA8 alone or in combined with NF-YA contributed to cancer progression, and could serve as novel potential therapeutic targets for HCC patients.
Effects of plasma-activated Ringer’s lactate solution on cancer cells: evaluation of genotoxicity
Authors: Liu Yang , Nakatsu Yoshimichi , Tanaka Hiromasa , Koga Kazunori , Ishikawa Kenji , Shiratani Masaharu , Hori Masaru 
Published: January 2023

Abstract
Background
Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer’s lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied.

Results
PAL has a low pH value and a high concentration of H2O2. H2O2 was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells.

Conclusion
PAL contains one or more active compounds or species in addition to H2O2 that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy.
Update on obesity and assisted reproductive technology
Authors: Elnashar Aboubakr Mohamed 
Published: January 2023

Abstract
Background
The effect of obesity on IVF consequences is still debatable.

Main body
Obesity is linked to a greater likelihood of maternal and fetal complications all through gestation, including miscarriage, premature births, fetal demise, and adverse pregnancy outcomes. There was no increase in chromosomal abnormality percentages with increasing body mass index (BMI), implying that poor oocyte “performance” in obese patients could be caused by variables other than chromosomal expertise. Ribonucleic acid (RNA)-sequence research showed molecular changes in the oocytes of obese patients. Each 5-unit rise in female BMI is attributed with both a 5% and 7% reduction in hazard for CPR and LBR, in both, and a 9% rise in the comparative chance of miscarriage. There is no clinical or moral mandate for establishing a societal BMI limit for rejecting a client or couple direct exposure to infertility treatment. Assessment with an interdisciplinary approach prior to an IVF treatment should be done to assess the safety of oocyte retrieval under anesthesia, taking into account variables including body mass index and comorbid conditions. On LBR, there is no convincing evidence of the significance of lifestyle modification for losing weight. There is contradictory information on the impacts of weight loss surgery on pregnancy outcomes. IVF success rate was unaffected by weight loss surgery.

Conclusion
Obesity has been linked to anovulation, decreased ovarian response to ovulatory prescription medications, changed oocyte and endometrial activity, an elevated risk of fertility problems, and reduced rates of live birth following IVF.
Reduced percentage of inconclusive reports and need for repeated fine needle aspirations in ultrasound versus free-hand parotid cytology
Authors: Prades Eduardo , Garlea-Robu Irina , Harris Andrew Steven , Mehdi Muhammad Zain , Zeitoun Hisham , Arya Arvind Kumar 
Published: January 2023

Abstract
Background
Fine needle aspiration cytology has been established as a minimally invasive, non-tumour seeding investigation of choice in the initial diagnostic pathway of parotid lesions. The purpose of this study was to compare the accuracy of fine needle aspiration cytology performed with and without ultrasound to determine whether one method should be preferred to the other. A retrospective review of all patients undergoing fine needle aspiration cytology with and without ultrasound for parotid masses in a large district general hospital between 2012 and 2016 was performed. Specificity, sensitivity, accuracy, positive and negative predictive value, percentage of inconclusive fine needle aspiration cytology and percentage of second fine needle aspiration cytology were determined for each group.

Results
A total of 397 fine needle aspiration cytology results were available for analysis. The numbers performed with ultrasound guidance and free-hand were roughly equal (208 (52.3%) versus 189 (47.7%)). The number of inconclusive fine needle aspiration cytology reports was significantly higher in the free-hand group (65/189 (34.4%)) than the ultrasound group (25/208 (12%)) (p < 0.0001). A significantly higher number of repeated fine needle aspiration cytology were undertaken in the free-hand group vs ultrasound group (43 vs 15, p < 0.0001); overall 7.2% of ultrasound-guided fine needle aspiration cytology required a second fine needle aspiration cytology, compared to 22.8% in the free-hand group. The sensitivity, specificity, positive and negative predictive values were all higher in the ultrasound group versus the free-hand group.

Conclusions
Ultrasound-guided fine needle aspiration cytology is superior to free-hand fine needle aspiration cytology in the investigation of parotid tumours. There is a significant benefit in reducing the number of inconclusive results and repeat fine needle aspiration cytology, and a potential benefit in improving the sensitivity and positive predictive value, when immediate cytology assessment of the sample quality is not performed.
Multidisciplinary patient-centred model of care for osteoarthritis: scoping review protocol—an initiative by the Egyptian Academy of Bone Health
Authors: El Miedany Yasser , Elwakil Walaa 
Published: January 2023

Abstract
Background
There is an evidence-practice gap in osteoarthritis (OA) management which has caused several patients living with the disease are receiving suboptimal medical care. Though there are several guidelines and treatment recommendations published, there is a real need to operationalise such evidence-based guidelines and facilitate their implementation by healthcare professionals in their local health systems

Main text
This work was carried out to outline a patient-centred multidisciplinary osteoarthritis care programme for knee and hip joint osteoarthritis that is applicable in standard clinical practice. A scoping review was conducted to identify an evidence-informed osteoarthritis management strategy, which outlines the optimal manner to treat patients living with osteoarthritis and can be implemented by healthcare professionals. The Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) extension for Scoping Reviews (PRISMA-ScR) checklist was used to guide the reporting of this review. Based on this, a “Model of Care” based on a patient-centred approach with shared decision-making to enhance the timely consideration of all treatment options (including non-pharmacological, pharmacological therapies, psychotherapy, rehabilitation as well as surgery) has been developed aiming to optimise the outcomes. The goals and principles have been identified as well as the key performance indices. An algorithm for the multidisciplinary management of osteoarthritis has been developed.

Conclusion
The developed osteoarthritis care programme (OACP) provided a “Model of Care” for people living with OA which can be implemented in standard practice. The results will give insight into the features, performance, results, and outcome measures assessed. It will also guide future research towards how “Model of Care” can be patient-centred and tailored to the individual medical status.
The association between Compulsory Community Treatment Order status and mortality in New Zealand
Authors: Beaglehole Ben , Newton-Howes Giles , Porter Richard , Frampton Chris 
Published: January 2023

Background
Compulsory Community Treatment Orders (CTOs) enable psychiatric medication without the need for consent. Careful scrutiny of outcomes including mortality is required to ensure compulsory treatment is evidence-based and ethical.

Aims
To report mortality for patients placed on CTOs and analyse data according to CTO status, mortality cause and diagnosis.

Method
Data for all patients placed under CTOs between 1 January 2009 and 31 December 2018 was provided by the Ministry of Health, New Zealand. Data included diagnostic and demographic information, dates of CTOs, and any dates and causes of death. Deaths were categorised into suicides, accidents and assaults, and medical causes. Mortality data are reported according to CTO status and diagnosis.

Results
A total of 14 726 patients were placed on CTOs over the study period, during which there were 1328 deaths. The mortality rate was 2.97 on and 2.31 off CTOs (rate ratio 1.29, 95% CI 1.14–1.45; P < 0.01). The mortality rate for accidents and assaults was 0.44 on and 0.25 off CTOs (rate ratio 1.73, 95% CI 1.23–2.42; P < 0.01). The mortality rate for medical causes was 2.33 on and 1.90 off CTOs (rate ratio 1.22, 95% CI 1.07–1.40; P < 0.01). The suicide rate was 0.20 on and 0.15 off of CTOs (rate ratio 1.33, 95% CI 0.81–2.12; P = 0.22).

Conclusions
Increased care and medication provided during compulsory treatment does not the modify the course of illness sufficiently to reduce mortality during CTOs. Higher mortality rates during CTO periods compared with non-CTO periods may reflect greater unwellness during CTOs.
Efficacy of sofpironium bromide gel on clozapine-induced hypersalivation in patients with treatment-resistant schizophrenia: double-blind, controlled crossover study
Authors: Amano Yuhei , Mazda Jun , Amano Koichi , Ohi Kazutaka , Shioiri Toshiki 
Published: January 2023

Background
Hypersalivation is a major side-effect of clozapine in patients with treatment-resistant schizophrenia.

Aims
We investigated the efficacy of topical anticholinergic formulation sofpironium bromide gel for improving hypersalivation in patients with treatment-resistant schizophrenia receiving clozapine.

Method
A double-blind, controlled crossover study was conducted with sofpironium bromide gel and a placebo gel to treat clozapine-induced hypersalivation in 16 patients with treatment-resistant schizophrenia. Patients were randomly divided between groups A and B (each n = 8). Group A was treated with sofpironium bromide gel for 6 weeks, followed by a 2-week washout period and 6 weeks of placebo gel, after which they were observed for another 2 weeks. In contrast, group B was treated with placebo gel for 6 weeks, followed by a 2-week washout period, 6 weeks of sofpironium bromide gel and a 2-week observation period. One-minute saliva volume, objective salivation ratings (Drooling Severity and Frequency Scale and Nocturnal Hypersalivation Rating Scale) and subjective salivation ratings (Visual Analogue Scale) were assessed every 2 weeks.

Results
All patients completed the trials. Three patients reported mild, spontaneously resolved skin itching. Compared with baseline values, the 1-min saliva volumes of both groups were significantly decreased by approximately 30% at the second week of sofpironium bromide gel treatment (P < 0.001), and significantly decreased by >40% at the fourth and sixth weeks of treatment (P < 0.001). The effects were maintained for over 2 weeks even after the treatment was discontinued.

Conclusions
We suggest that sofpironium bromide gel is effective in treating clozapine-induced hypersalivation in patients with treatment-resistant schizophrenia.
The impact of the COVID-19 pandemic on perinatal services and maternal mental health in the UK
Authors: Kasaven Lorraine S. , Raynaud Isabel , Jalmbrant Maria , Joash Karen , Jones Benjamin P. 
Published: January 2023

Background
COVID-19 has created many challenges for women in the perinatal phase. This stems from prolonged periods of lockdowns, restricted support networks and media panic, alongside altered healthcare provision.

Aims
We aimed to review the evidence regarding the psychological impact on new and expecting mothers following changes to antenatal and postnatal service provision within the UK throughout the pandemic.

Method
We conducted a narrative literature search of major databases (PubMed, Medline, Google Scholar). The literature was critically reviewed by experts within the field of antenatal and perinatal mental health.

Results
Changes to service provision, including the introduction of telemedicine services, attendance of antenatal appointments without partners or loved ones, and lack of support during the intrapartum period, are associated with increased stress, depression and anxiety. Encouraging women and their partners to engage with aspects of positive psychology through newly introduced digital platforms and virtual service provision has the potential to improve access to holistic care and increase mental well-being. An online course, designed by Imperial College Healthcare NHS Trust in response to changes to service provision, focuses on postnatal recovery inspiration and support for motherhood (PRISM) through a 5-week programme. So far, the course has received positive feedback.

Conclusions
The pandemic has contributed to increased rates of mental illness among pregnant and new mothers in the UK. Although the long-term implications are largely unpredictable, it is important to anticipate increased prevalence and complexity of symptoms, which could be hugely detrimental to an already overburdened National Health Service.
The influence of the optimal velocity deviation on the traffic evolution process under lane change in two-lane lattice model
Authors: Li Xiaoqin , Jin Can , Peng Guanghan 
Published: January 2023

Abstract
In a real traffic environment, there is always a certain deviation between the real traffic information and the expected traffic information, which definitely has an important impact on traffic flow. Consequently, the optimal velocity deviation effect (OVDE) is taken into account to build a novel lattice model on two lanes. Moreover, the influence of OVDE on the linear stable condition is investigated, which shows that the OVDE plays a positive stable effect in two-lane traffic flow. Also, the OVDE can relieve more traffic congestion on two lanes via numerical simulations concerning the density and the hysteresis loop.
A Moral Duty to Uphold the Family Farm”: The Religious Response to the 1980s Farm Crisis
Authors: Anderson Wayne 
Published: January 2023

Abstract
The 1980s Farm Crisis began receiving national attention in the middle of the decade in large part because of Hollywood films such as Country and the star-studded Farm Aid concert. However, religious leaders and organizations had already started to warn of an impending crisis as early as 1978. They were motivated by a scriptural belief in land stewardship as well as empathy for their struggling rural parishioners. Throughout a series of noteworthy conferences, position statements, and protest actions coordinated by religious organizations across the denominational spectrum, Midwest-based leaders increasingly attempted to raise awareness, influence public policy, and bear witness to the escalating crisis. This article documents these ecumenical efforts during a pivotal time in America's rural history and provides a new angle on the farm crisis of the 1980s.
Expanding Educational Frontiers in Neurosurgery: Current and Future Uses of Augmented Reality
Authors: Olexa Joshua , Cohen Jonathan , Alexander Tyler , Brown Cole , Schwartzbauer Gary , Woodworth Graeme F. 
Published: January 2023

BACKGROUND:
Augmented reality (AR) technology is a new and promising option to advance and expand neurosurgical training because of recent advances in computer vision technology, improved AR software and hardware, and growing acceptance of this technology in clinical practice.

OBJECTIVE:
To analyze the current status of AR use cases with the goal of envisioning future uses of AR in neurosurgical education.

METHODS:
Articles applying to AR technology use in neurosurgical education were identified using PubMed, Google Scholar, and Web of Science databases following the Preferred Reporting Items of Systematic Reviews and Meta-Analyses guidelines. Articles were included for review based on applicable content related to neurosurgical or neuroanatomy training. Assessment of literature quality was completed using standardized MERSQI scoring.

RESULTS:
The systematic search identified 2648 unique articles. Of these, 12 studies met inclusion criteria after extensive review. The average MERSQI score was 10.2 (SD: 1.7). The most common AR platform identified in this study was the Microsoft Hololens. The primary goals of the studies were to improve technical skills and approaches to surgical planning or improve understanding of neuroanatomy.

CONCLUSION:
Augmented reality has emerged as a promising training tool in neurosurgery. This is demonstrated in the wide range of cases in technical training and anatomic education. It remains unclear how AR-based training compares directly with traditional training methods; however, AR shows great promise in the ability to further enhance and innovate neurosurgical education and training.
Preoperative Brain Functional Connectivity Improve Predictive Accuracy of Outcomes After Revascularization in Moyamoya Disease
Authors: Hu Junwen , Wang Yongjie , Zhu Yuhan , Li Yin , Chen Jingyin , Zhang Yifan , Xu Duo , Bai Ruiliang , Wang Lin 
Published: January 2023

BACKGROUND:
In patients with moyamoya disease (MMD), focal impairments in cerebral hemodynamics are often inconsistent with patients' clinical prognoses. Evaluation of entire brain functional networks may enable predicting MMD outcomes after revascularization.

OBJECTIVE:
To investigate whether preoperative brain functional connectivity could predict outcomes after revascularization in MMD.

METHODS:
We included 34 patients with MMD who underwent preoperative MRI scanning and combined revascularization surgery. We used region of interest analyses to explore the differences in functional connectivity for 90 paired brain regions between patients who had favorable outcomes 1 year after surgery (no recurrent stroke, with improved preoperative symptoms, or modified Rankin Scale [mRS]) and those who had unimproved outcomes (recurrent stroke, persistent symptoms, or declined mRS). Variables, including age, body mass index, mRS at admission, Suzuki stage, posterior cerebral artery involvement, and functional connectivity with significant differences between the groups, were included in the discriminant function analysis to predict patient outcomes.

RESULTS:
Functional connectivity between posterior cingulate cortex and paracentral lobule within the right hemisphere, and interhemispheric connection between superior parietal gyrus and middle frontal gyrus, precuneus and middle cingulate cortex, cuneus and precuneus, differed significantly between the groups (P < .001, false discovery rate corrected) and had the greatest discriminant function in the prediction model. Although clinical characteristics of patients with MMD showed great accuracy in predicting outcomes (64.7%), adding information on functional connections improved accuracy to 91.2%.

CONCLUSION:
Preoperative functional connectivity derived from rs-fMRI may be an early hallmark for predicting patients' prognosis after revascularization surgery for MMD.
Class of 1923: looking back at the authors of JEB's first issue
Authors: Hankins Laura E. , Rutledge Charlotte E. 
Published: January 2023

ABSTRACT
Journal of Experimental Biology was launched in 1923 as The British Journal of Experimental Biology, with a single issue being published in the October of that year. As we celebrate our centenary, we look back at that first issue and the zoologists publishing their work in the new journal, and draw comparisons to the JEB that we know today. Much has changed since the publication of the first issue of JEB, in the worlds of both science and publishing, and we eagerly anticipate the next 100 years of discovery.