bluuebunny/crossref_metadata_2025_split huggingface dataset:
RANCANG BANGUN PROTOTYPE TURBIN ANGIN GUNA MENENTUKAN PERBANDINGAN EFISIENSI YANG DIHASILKAN DARI JUMLAH BLADE 8 DAN JUMLAH BLADE 4
Authors: Hasibuan Sirdo , Susilo  , Widodo Bambang , Purba Robinson 
Published: August 2023

Dalam penelitian ini dirancang satu turbin dengan mengamati perbandingan jumlah sudu pada turbin terhadap ujuk kerja turbin seperti; daya dan efisiensi. Turbin terdiri dari blade dengan bentuk setengah silinder dengan luas penampang A = 0,246176 m2. Turbin angin ini didesain dengan diameter putar 170 cm dan perbandingan transmisi pada turbin dan generator yaitu 4:1. Hasil penelitian menunjukkan bahwa perbandingan jumlah sudu 8 dan 4, peforma terbaik dihasilkan oleh turbin angin jumlah sudu 8 dengan peningkatan 1,5 kali dibandingkan jumlah sudu 4. Kincir angin savonius dengan jumlah sudu 4 menghasilkan efisiensi paling tinggi sebesar 5% dan kincir angin savonius dengan jumlah sudu 8 menghasilkan efisiensi paling tinggi sebesar 10%. Pada kincir Angin Savonius sudu 4 tidak dapat berputar pada kecepatan angin rendah, hanya mampu berputar pada kecepatan angin angin ÷4 m/s . Hal ini diakibatkan karena celah antar sudu yang lebar, sehingga besarnya upwind dan downwind pada kincir sudu 4 sama besarnya. Daya Mekanik tertinggi Turbin angin savonius 8 sudu adalah 162, 85 watt dengan kecepatan angin 5,13 m/s pada percobaan tanpa beban dan daya mekanik terendah turbin angin savonius terjadi pada 4 bilah dengan beban sebesar 30,80 watt dengan kecepatan angin 3,71 m/s
PETA JALAN PENDIDIKAN REKAYASA ENERGI BAGI INDONESIA -- SEBUAH PROPOSAL
Authors: Lisapaly Leonard , Pangaribuan Togar Harapan , Soebagio Atmonobudi 
Published: August 2023

Indonesia has ratified 17 Sustainable Development Goals (SDG) recommended by the United Nations to all countries. The development itself cannot be carried out without the availability of electricity and fuel. Unfortunately, most of the electricity and transportation depend on fossil fuels, which will run out in the next 30 years. Besides its diminishing reserves, fossil fuels are the largest contributor to CO2 emissions in the world. To develop electricity from renewable energy, Indonesia needs to prepare its experts and operators through mid and high education to carry out research, operations and future development. This paper offers a road map for higher education and its direction of research and development in the field of renewable energy engineering.
PENGUKURAN TOTAL HARMONIC DISTORTION (THD) TERHADAP LAMPU HEMAT ENERGI (LHE) DAN LIGHT-EMITTING DIODE (LED)
Authors: Siahaan Fernando Jeremia , Silalahi Eva Magdalena , Widodo Bambang , Purba Robinson 
Published: August 2023

Abstract.
Along with the rapid development of power electronics technology which is also useful for saving electrical energy. This technology is also used in lighting such as energy saving lamps (LHE) and light-emitting diode (LED). However, LHE and LED are non-linear loads that cause electrical power quality problems, namely producing voltage and current waveforms that are no longer sinusoidal or distorted waveforms. Distorted waves cause harmonic waves which have a frequency multiple of the fundamental frequency. The sum of the effective values of the harmonic components of the distorted waveform is calculated in terms of total harmonic distortion (THD). This study found that the THD value of the voltage generated by the LHE and LED loads was equal is 0.80% -1.3%, while the current THD generated by the LHE load was 68.57% - 78.68% and the LED was 72, 15% - 86.04%. The current THD value generated by the LHE and LED loads does not fulfil the IEEE Std 519-2014 harmornic distortion limits. The use of LHE and LED lamps simultaneously will reduce the resulting current THD value by 3% to 9%.
A novel 3D biofabrication strategy to improve cell proliferation and differentiation of human Wharton’s jelly mesenchymal stromal cells for cell therapy and tissue engineering
Authors: Blanco-Elices Cristina , Oruezabal Roke Iñaki , Sánchez-Porras David , Chato-Astrain Jesús , Campos Fernando , Alaminos Miguel , Garzón Ingrid , Campos Antonio 
Published: August 2023

Purpose: Obtaining sufficient numbers of cells in a short time is a major goal of cell culturing in cell therapy and tissue engineering. However, current bidimensional (2D) culture methods are associated to several limitations, including low efficiency and the loss of key cell differentiation markers on cultured cells.Methods: In the present work, we have designed a novel biofabrication method based on a three-dimensional (3D) culture system (FIBRIAGAR-3D). Human Wharton’s jelly mesenchymal stromal cells (HWJSC) were cultured in 3D using 100%, 75%, 50%, and 25% concentrations of fibrin-agarose biomaterials (FA100, FA75, FA50 and FA25 group) and compared with control cells cultured using classical 2D systems (CTR-2D).Results: Our results showed a significant increase in the number of cells generated after 7 days of culture, with cells displaying numerous expansions towards the biomaterial, and a significant overexpression of the cell proliferation marker KI67 was found for the FA75 and FA100 groups. TUNEL and qRT-PCR analyses demonstrated that the use of FIBRIAGAR-3D was not associated with an induction of apoptosis by cultured cells. Instead, the 3D system retained the expression of typical phenotypic markers of HWJSC, including CD73, CD90, CD105, NANOG and OCT4, and biosynthesis markers such as types-I and IV collagens, with significant increase of some of these markers, especially in the FA100 group. Finally, our analysis of 8 cell signaling molecules revealed a significant decrease of GM-CSF, IFN-g, IL2, IL4, IL6, IL8, and TNFα, suggesting that the 3D culture system did not induce the expression of pro-inflammatory molecules.Conclusion: These results confirm the usefulness of FIBRIAGAR-3D culture systems to increase cell proliferation without altering cell phenotype of immunogenicity and opens the door to the possibility of using this novel biofabrication method in cell therapy and tissue engineering of the human cornea, oral mucosa, skin, urethra, among other structures.
Gut microbiota and eye diseases: a bibliometric study and visualization analysis
Authors: Fu Xiangyu , Tan Haishan , Huang Ling , Chen Wenyue , Ren Xiang , Chen Danian 
Published: August 2023

IntroductionRecently the role of gut microbial dysbiosis in many ocular disorders, including but not limited to uveitis, age-related macular degeneration (AMD), diabetic retinopathy (DR), dry eye, keratitis and orbitopathy is a hot research topic in the field. Targeting gut microbiota to treat these diseases has become an unstoppable trend. Bibliometric study and visualization analysis have become essential methods for literature analysis in the medical research field. We aim to depict this area's research hotspots and future directions by bibliometric software and methods.MethodsWe search all the related publications from the Web of Science Core Collection. Then, CiteSpace was applied to analyze and visualize the country distributions, dual-map overlay of journals, keyword bursts, and co-cited references. VOSviewer was employed to identify authors, co-cited authors, journals and co-cited journals and display the keyword co-occurrence networks.ResultsA total of 284 relevant publications were identified from 2009 to 2023. The number of studies has been small in the first five years and has grown steadily since 2016. These studies were completed by 1,376 authors from 41 countries worldwide, with the United States in the lead. Lin P has published the most papers while Horai R is the most co-cited author. The top journal and co-cited journal are both Investigative Ophthalmology &amp; Visual Science. In the keyword co-occurrence network, except gut microbiota, inflammation becomes the keyword with the highest frequency. Co-citation analyses reveal that gut dysbiosis is involved in common immune- and inflammation-mediated eye diseases, including uveitis, diabetic retinopathy, age-related macular degeneration, dry eye, and Graves' orbitopathy, and the study of microbiomes is no longer limited to the bacterial populations. Therapeutic strategies that target the gut microbiota, such as probiotics, healthy diet patterns, and fecal microbial transplantation, are effective and critical to future research.ConclusionsIn conclusion, the bibliometric analysis displays the research hotspots and developmental directions of the involvement of gut microbiota in the pathogenesis and treatment of some ocular diseases. It provides an overview of this field's dynamic evolution and structural relationships.
Transcriptomic analyses of ovarian clear-cell carcinoma with concurrent endometriosis
Authors: Collins Kaitlyn E. , Wang Xiyin , Klymenko Yuliya , Davis Noah B. , Martinez Maria C. , Zhang Chi , So Kaman , Buechlein Aaron , Rusch Douglas B. , Creighton Chad J. , Hawkins Shannon M. 
Published: August 2023

IntroductionEndometriosis, a benign inflammatory disease whereby endometrial-like tissue grows outside the uterus, is a risk factor for endometriosis-associated ovarian cancers. In particular, ovarian endometriomas, cystic lesions of deeply invasive endometriosis, are considered the precursor lesion for ovarian clear-cell carcinoma (OCCC).MethodsTo explore this transcriptomic landscape, OCCC from women with pathology-proven concurrent endometriosis (n = 4) were compared to benign endometriomas (n = 4) by bulk RNA and small-RNA sequencing.ResultsAnalysis of protein-coding genes identified 2449 upregulated and 3131 downregulated protein-coding genes (DESeq2, P&lt; 0.05, log2 fold-change &gt; |1|) in OCCC with concurrent endometriosis compared to endometriomas. Gene set enrichment analysis showed upregulation of pathways involved in cell cycle regulation and DNA replication and downregulation of pathways involved in cytokine receptor signaling and matrisome. Comparison of pathway activation scores between the clinical samples and publicly-available datasets for OCCC cell lines revealed significant molecular similarities between OCCC with concurrent endometriosis and OVTOKO, OVISE, RMG1, OVMANA, TOV21G, IGROV1, and JHOC5 cell lines. Analysis of miRNAs revealed 64 upregulated and 61 downregulated mature miRNA molecules (DESeq2, P&lt; 0.05, log2 fold-change &gt; |1|). MiR-10a-5p represented over 21% of the miRNA molecules in OCCC with endometriosis and was significantly upregulated (NGS: log2fold change = 4.37, P = 2.43e-18; QPCR: 8.1-fold change, P&lt; 0.05). Correlation between miR-10a expression level in OCCC cell lines and IC50 (50% inhibitory concentration) of carboplatin in vitro revealed a positive correlation (R2 = 0.93). MiR-10a overexpression in vitro resulted in a significant decrease in proliferation (n = 6; P&lt; 0.05) compared to transfection with a non-targeting control miRNA. Similarly, the cell-cycle analysis revealed a significant shift in cells from S and G2 to G1 (n = 6; P&lt; 0.0001). Bioinformatic analysis predicted that miR-10a-5p target genes that were downregulated in OCCC with endometriosis were involved in receptor signaling pathways, proliferation, and cell cycle progression. MiR-10a overexpression in vitro was correlated with decreased expression of predicted miR-10a target genes critical for proliferation, cell-cycle regulation, and cell survival including [SERPINE1 (3-fold downregulated; P&lt; 0.05), CDK6 (2.4-fold downregulated; P&lt; 0.05), and RAP2A (2-3-fold downregulated; P&lt; 0.05)].DiscussionThese studies in OCCC suggest that miR-10a-5p is an impactful, potentially oncogenic molecule, which warrants further studies.
Alzheimer’s disease and its associated risk of bone fractures: a narrative review
Authors: Zhou Bing-Na , Zhang Qian , Li Mei 
Published: August 2023

BackgroundAlzheimer’s disease (AD) is a neurodegenerative disorder that is the major cause of dementia in the aged population. Recent researches indicate that patients with AD have a significantly increased fracture risk, but the pathological mechanisms are still unclear.ObjectiveWe systematically reviewed studies regarding bone fracture risk in AD to uncover links between the pathologies of osteoporosis and AD.MethodsWe searched the literature using the databases of PubMed, Web of Science, Embase and Cochrane Library. Studies were included if they evaluated bone fracture risk in AD patients and if they explored the pathogenesis and prevention of bone fractures in these patients.ResultsAD patients had a significantly higher risk of bone fractures than age-matched controls. Multiple factors contributed to the increased risk of bone fractures in AD patients, including the direct effects of amyloid pathology on bone cells, abnormal brain-bone interconnection, Wnt/β-catenin signalling deficits, reduced activity, high risk of falls and frailty, and chronic immune activity. Exercise, prevention of falls and fortified nutrition were beneficial for reducing the fracture risk in AD patients. However, the efficacy of anti-osteoporotic agents in preventing bone fractures should be further evaluated in AD patients as corresponding clinical studies are very scarce.ConclusionAlzheimer’s disease patients have increased bone fracture risk and decreased bone mineral density owing to multiple factors. Assessment of anti-osteoporotic agents’ efficacy in preventing bone fractures of AD patients is urgently needed.
Systematic establishment and verification of an epithelial-mesenchymal transition gene signature for predicting prognosis of oral squamous cell carcinoma
Authors: Ai Jun , Tan Yaqin , Liu Bo , Song Yuhong , Wang Yanqin , Xia Xin , Fu Qicheng 
Published: August 2023

Objective: Epithelial-mesenchymal transition (EMT) is linked to an unfavorable prognosis in oral squamous cell carcinoma (OSCC). Here, we aimed to develop an EMT gene signature for OSCC prognosis.Methods: In TCGA dataset, prognosis-related EMT genes with p &lt; 0.05 were screened in OSCC. An EMT gene signature was then conducted with LASSO method. The efficacy of this signature in predicting prognosis was externally verified in the GSE41613 dataset. Correlations between this signature and stromal/immune scores and immune cell infiltration were assessed by ESTIMATE and CIBERSORT algorithms. GSEA was applied for exploring significant signaling pathways activated in high- and low-risk phenotypes. Expression of each gene was validated in 40 paired OSCC and normal tissues via RT-qPCR.Results: A prognostic 9-EMT gene signature was constructed in OSCC. High risk score predicted poorer clinical outcomes than low risk score. ROCs confirmed the well performance on predicting 1-, 3- and 5-year survival. Multivariate cox analysis revealed that this signature was independently predictive of OSCC prognosis. The well predictive efficacy was validated in the GSE41613 dataset. Furthermore, this signature was distinctly related to stromal/immune scores and immune cell infiltration in OSCC. Distinct pathways were activated in two subgroups. After validation, AREG, COL5A3, DKK1, GAS1, GPX7 and PLOD2 were distinctly upregulated and SFRP1 was downregulated in OSCC than normal tissues.Conclusion: Our data identified and verified a robust EMT gene signature in OSCC, which provided a novel clinical tool for predicting prognosis and several targets against OSCC therapy.
GBP2 promotes M1 macrophage polarization by activating the notch1 signaling pathway in diabetic nephropathy
Authors: Li Xiaohui , Liu Jialu , Zeng Mengru , Yang Kexin , Zhang Shumin , Liu Yifei , Yin Xiangxiang , Zhao Chanyue , Wang Wenpeng , Xiao Li 
Published: August 2023

BackgroundDiabetic nephropathy (DN) is one of the most common diabetic complications, which has become the primary cause of end-stage renal disease (ESRD) globally. Macrophage infiltration has been proven vital in the occurrence and development of DN. This study was designed to investigate the hub genes involved in macrophage-mediated inflammation of DN via bioinformatics analysis and experimental validation.MethodsGene microarray datasets were obtained from the Gene Expression Omnibus (GEO) public website. Integrating the CIBERSORT, weighted gene co-expression network analysis (WGCNA) and DEGs, we screened macrophage M1-associated key genes with the highest intramodular connectivity. Subsequently, the Least Absolute Shrinkage and Selection Operator (LASSO) regression was utilized to further mine hub genes. GSE104954 acted as an external validation to predict the expression levels and diagnostic performance of these hub genes. The Nephroseq online platform was employed to evaluate the clinical implications of these hub genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed to elucidate the dominant biological functions and signal pathways. Finally, we conducted experiments to verify the role of GBP2 in M1 macrophage-mediated inflammatory response and the underlying mechanism of this role.ResultsSixteen DEGs with the highest connectivity in M1 macrophages-associated module (paleturquoise module) were determined. Subsequently, we identified four hub genes through LASSO regression analysis, including CASP1, MS4A4A, CD53, and GBP2. Consistent with the training set, expression levels of these four hub genes manifested memorably elevated and the ROC curves indicated a good diagnostic accuracy with an area under the curve of greater than 0.8. Clinically, enhanced expression of these four hub genes predicted worse outcomes of DN patients. Given the known correlation between the first three hub genes and macrophage-mediated inflammation, experiments were performed to demonstrate the effect of GBP2, which proved that GBP2 contributed to M1 polarization of macrophages by activating the notch1 signaling pathway.ConclusionOur findings detected four hub genes, namely CASP1, MS4A4A, CD53, and GBP2, may involve in the progression of DN via pro-inflammatory M1 macrophage phenotype. GBP2 could be a promising prognostic biomarker and intervention target for DN by regulating M1 polarization.
Deciphering the crosstalk of immune dysregulation between COVID-19 and idiopathic inflammatory myopathy
Authors: Zhang Zhao , Tao Weidong , Cheng Debin , Qin Marong , Fu Jun , Liu Dong 
Published: August 2023

BackgroundThe coronavirus disease (COVID-19) pandemic is a serious threat to public health worldwide. Growing evidence reveals that there are certain links between COVID-19 and autoimmune diseases; in particular, COVID-19 and idiopathic inflammatory myopathies (IIM) have been observed to be clinically comorbid. Hence, this study aimed to elucidate the molecular mechanisms of COVID-19 and IIM from a genomic perspective.MethodsWe obtained transcriptome data of patients with COVID-19 and IIM separately from the GEO database and identified common differentially expressed genes (DEGs) by intersection. We then performed functional enrichment, PPI, machine learning, gene expression regulatory network, and immune infiltration analyses of co-expressed genes.ResultsA total of 91 common genes were identified between COVID-19 and IIM. Functional enrichment analysis revealed that these genes were mainly involved in immune dysregulation, response to external stimuli, and MAPK signaling pathways. The MCODE algorithm recognized two densely linked clusters in the common genes, which were related to inflammatory factors and interferon signaling. Subsequently, three key genes (CDKN1A, IFI27, and STAB1) were screened using machine learning to predict the occurrence of COVID-19 related IIM. These key genes exhibited excellent diagnostic performance in both training and validation cohorts. Moreover, we created TF-gene and miRNA-gene networks to reveal the regulation of key genes. Finally, we estimated the relationship between key genes and immune cell infiltration, of which IFI27 was positively associated with M1 macrophages.ConclusionOur work revealed common molecular mechanisms, core genes, potential targets, and therapeutic approaches for COVID-19 and IIM from a genomic perspective. This provides new ideas for the diagnosis and treatment of COVID-19 related IIM in the future.
A comprehensive rhythmicity analysis of host proteins and immune factors involved in malaria pathogenesis to decipher the importance of host circadian clock in malaria
Authors: Rankawat Sourbh , Kundal Kavita , Chakraborty Shreyayukta , Kumar Rahul , Ray Sandipan 
Published: August 2023

BackgroundCircadian rhythms broadly impact human health by regulating our daily physiological and metabolic processes. The circadian clocks substantially regulate our immune responses and susceptibility to infections. Malaria parasites have intrinsic molecular oscillations and coordinate their infection cycle with host rhythms. Considering the cyclical nature of malaria, a clear understanding of the circadian regulations in malaria pathogenesis and host responses is of immense importance.MethodsWe have thoroughly investigated the transcript level rhythmic patterns in blood proteins altered in falciparum and vivax malaria and malaria-related immune factors in mice, baboons, and humans by analyzing datasets from published literature and comprehensive databases. Using the Metascape and DAVID platforms, we analyzed Gene Ontology terms and physiological pathways associated with the rhythmic malaria-associated host immune factors.ResultsWe observed that almost 50% of the malaria-associated host immune factors are rhythmic in mice and humans. Overlapping rhythmic genes identified in mice, baboons, and humans, exhibited enrichment (Q &lt; 0.05, fold-enrichment &gt; 5) of multiple physiological pathways essential for host immune and defense response, including cytokine production, leukocyte activation, cellular defense, and response, regulation of kinase activity, B-cell receptor signaling pathway, and cellular response to cytokine stimulus.ConclusionsOur analysis indicates a robust circadian regulation on multiple interconnected host response pathways and immunological networks in malaria, evident from numerous rhythmic genes involved in those pathways. Host immune rhythms play a vital role in the temporal regulation of host-parasite interactions and defense machinery in malaria.