bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunological inflammatory factors in patients diagnosed with COVID-19
Authors: Abbas Mohammed S. , Ahmed Ahmed Ghaith , Ali Sura Qusay , AL-Rubaii Bahaa Abdullah Lafttah 
Published: February 2023

Introduction and Aim: Due to the Coronavirus outbreaks, the SARS-CoV-2, also known as COVID-19, has claimed several lives around the world, with the majority of them being elderly, suffering from underlying chronic illnesses, or living in vulnerable conditions. This study aimed to find the immunological factors CD-79, CD-4, IL-2, and TNF-B in COVID-19 patients utilizing nucleocapsid-(N), a protein structure that interacts with genomic RNA to create complexes.

Materials and Methods: SARS-COV-2 infection was detected using RT-PCR. The serum levels of IL-2 and TNF-B, as well as the concentrations of CD4 and CD79, were measured. This study included 100 COVID-19 patients.

Results: The results showed that the serum concentration of TNF-beta and IL-2 in COVID19 patients was significantly higher than that in the general population (with acute and moderate illness) when compared to normal control groups (p 0.05). COVID-19 patients reported higher levels of CD79 as well as CD4 expression than healthy control groups in a study of activated markers.

Conclusion: Infection with SARS-COV-2 has a high impact on various immunological and inflammatory markers in patients.
Study of the association of toll like-4 receptor gene polymorphism (rs1927911) and susceptibility to urinary tract infection among Iraqi patients
Authors: Allami Risala H. , Abdullah Muhanad Najm , K. Al-Ani Basma , AlChalabi Rawaa , S. Hamzah Sawsan 
Published: February 2023

Introduction and Aim: Toll-like receptors (TLRs) are cell innate immune receptors that explore microbial molecules. A quantity of single-nucleotide polymorphisms (SNPs) within the TLR genes have been related with altered susceptibility to infectious, inflammatory, and allergic diseases suggesting: This study was conducted to investigate the relationship between genetic polymorphism in TLR4 gene (rs1927911) with susceptibility to UTIs in a sample of Iraqi patients.

Materials and Methods: Blood samples were collected from 220 patients who had UTIs and 69 healthy subjects as control group, during the period from October 2021 to March 2022, at the Rheumatology Department of Clinic Consultant at Al-Yarmouk Hospital. The genetic study included an extraction of genomic DNA and PCR technique, to detect the role of TLR4 gene in the variation site (rs1927911) as a risk factor for the incidence of UTIs.

Results: The results of genotype distribution and allele frequency of rs1927911 indicated, CC (18.6 %), CT (70 %) and TT (11.4 %) among the patients while 57.9 % for CC and 26.1%; 16% for CT, TT genotypes respectively of healthy control. Heterozygous (CT) may be considered as a risk factor among UTIs patients. The results revealed significant differences between UTIs patients and healthy controls in serum levels TLR4, IL-6 IL-2, TNF-alpha and IL-10 at P 0.005. In addition, the data found a highly significant difference of serum level of TLR4 protein with patients who have a carrier CT genotype.

Conclusion: our findings indicated that the carriers of CT genotype of TLR4 (rs1927911) were significantly more prone to UTIs.
Expression and cytotoxic effect of recombinant Newcastle Disease Virus (rNDV) vector expressing enhanced green fluorescent gene in JHH5 cell line
Authors: Rasoul Lubna Muhi , H. Allami Risala , Alshibib Ammar Luay , Al-Rubaii Bahaa Abdullah laftaah , Hadi Sale Tahreer 
Published: February 2023

Introduction and Aim: In medicine, the term ‘cancer’ is used to describe a group of disorders defined by uncontrolled cell growth and the ability to invade and metastasize to other organs. As a result of the body's ability to fight conventional cancer treatments, it is essential to look for alternative therapeutic avenues. Recent years have seen a rise in the application of reverse genetics techniques including the Newcastle disease virus as an oncolytic agent in tumor models of immunocompetent malignancy. In preclinical studies, recombinant NDV (rNDV-GFP) that expresses foreign genes was shown to be more effective in cancer therapy. The goal of this study was to examine the in vitro anticancer effects of GFP-expressing, genetically modified Newcastle disease virus (rClone3-GFP) strains on the JHH5 hepatocellular carcinoma cell line.

Materials and Methods: To generate a recombinant NDV, the GFP gene was inserted into the genome of an NDV strain (NDVClone30) by reverse genetics technology, where it now resides in the region of the genome occupied by the F and HN genes (rNDV-GFP). Using the MTT assay, we evaluated rNDV-GFP for its oncolytic potential against JHH5 cancer cells. Cytopathic effects were analyzed using fluorescent and light microscopy.

Results: We observed that 96 hours post infection, the recombinant virus was capable of inducing tumor cell death in a time-dependent way.

Conclusion: Recombinant NDV strains expressing GFP, demonstrated good results in inhibiting tumor growth. Our results pave the door for the application of recombinant NDV as a viral vector for the treatment of liver cancer.
Effect of integrated approach of yoga therapyonloneliness in elderly: An interventional study
Authors: Panigrahi Manorama , Shree Prativa , Prasad Swain Dinesh 
Published: February 2023

Introduction and Aim: A person must be adaptable and learn new coping mechanisms to deal with the changes that are common to these stages of life to cope with the changes that come with getting older. But various research also give evidence that loneliness, depressions are very much common during old age which is a significant contributor to suicide and suicide attempts. Incorporating Integrated Approach of Yoga Therapy, one can able to reestablish the link betweenhis mind, body with the present moment, alleviates loneliness, and boosts self-esteem. The objective of the study to know the effect of integrated approach of yoga therapy on loneliness of elderly people.

Materials and Methods: The study was carried out by the researcher taking 50 older people between the age group of 60 to 80 following the inclusion and exclusion criteria and through purposive sampling method. The study was done by using one sample group for pre and post-test assessment. All the participants had undergone a practice session of 12weeks in the nearby Temple premises. They were instructed to lead regular lives while taking their medications as prescribed and to maintain a healthy eating pattern. The UCLA questionnaire was used to evaluate loneliness before and after practice.

Results: After regular three months practice, the UCLA scale showed significantly decreasing trend. The sample average of the pre- and post-test data showed significant differences enough to be statistically relevant.

Conclusion: Adopting IAYT into their regular lifestyle, seniors can reduce their loneliness and enhance self- esteem.
Extracellular E-selectin levels and its association with insulin resistance: Putative role of E-selectin as a biomarker of oxidative stress linked vascular manifestations in diabetic nephropathy
Authors: Farid Babu Meka , A. R. Srinivasan , G. V. Benerji , M. S. Ravikumar 
Published: February 2023

Introduction and aim: E-selectin, a cell adhesion molecule depicting nexus with oxidative stress in hyperglycemia has been documented in several other clinical conditions. The present study centers around the putative role of E-selectin as a biomarker of diabetic nephropathy linked to oxidative stress.

Materials and Methods: 150 type 2 diabetics in the age group of 35 - 50 years were classified into three groups, comprising 50 each, based on albuminuria. Fifty healthy age and gender matched individuals constituted the control. Extracellular E-selectin and Insulin were quantitated appropriately. Measurement of HbA1c and microalbumin were also enabled. Routine biochemistry analyses were undertaken, based on established automated procedures. The study was initiated only following approval accorded by the Institutional Ethics Committee.

Results: E-selectin levels in serum were significantly associated with Urine Albumin-to-Creatinine Ratio (UACR) and Insulin resistance in the light of estimated Glomerular Filtration Rate.

Conclusion: E-selectin levels could thus act as a putative biomarker of diabetic nephropathy linked to oxidative stress, as observed in insulin resistant type 2 diabetes.
Glycosaminoglycans from fish waste alleviate high fat diet induced obesity via modulating plasma biomarkers and fatty acid profile of obese C57BL/6 mice
Authors: V. Geetha , Kumar G. Suresh 
Published: February 2023

Introduction and Aim: Glycosaminoglycans are sulphated polysaccharides which shows various biological activities including anti-obesity, anti-inflammation etc., Extracted glycosaminoglycans (e-GAGs) from mackerel fish waste was studied for their potential as an anti-obesity molecule by in-vivo mechanisms. An attempt was made to study their beneficial effects in regulating plasma biomarkers and fatty acids of liver and fecal matter.

Materials and Methods: In-vivo studies were conducted on male C57BL/6 mice (20-22g, 6-8 weeks of age). The animal studies had the IAEC (150/2019) clearance from CSIR-CFTRI, Mysuru. Animals were fed with a high fat diet and the e-GAGs treatment was at 0.4 and 0.8% through diet. Plasma analysis was done with commercially available kits; fatty acids were analyzed by gas chromatography as fatty acid methyl esters. Histopathology was carried out with H&E staining.

Results: Plasma biomarkers like SGOT, SGPT, ALP, creatinine and LDH were reduced in the treatment groups in dose dependent manner compared to high fat fed groups. SFA in the liver was highest in the high fat fed group (54.25%), ameliorated to 50.25 and 48.35% with the e-GAGs fed at 0.4 and 0.8% through diet respectively. Fecal fatty acid composition showed the higher (p<0.05) elimination of SFA in the treatment groups. Tissue histopathology of spleen, kidney and pancreas showed the highest damage in the high fat fed group and normalized with the e-GAGs treatment.

Conclusion: e-GAGs from fish waste could be beneficial in modulating obesity via modulating fatty acid composition and tissue protection from the obesity induced damage and benefit as a potent anti-obesity molecule.
Augmentation of in vitro cytotoxic potentiality of crude methanolic leaf extract of Olea dioica as a pivotal anticancer source
Authors: Krishnappa Srinivasa , Karthik Yalpi , Shantaram Manjula 
Published: February 2023

Introduction and Aim: Tumor is the major cause of death world-wide, with an approximate 10 million fatalities in 2020, or almost one in every six deaths. The concerns with the current commercially available anticancer medications' low selectivity and persistent side effects have driven the research of safer and more effective chemotherapeutic drugs. The current work is focused on determining the cytotoxic potential of crude methanolic extract of Olea dioica leaves against various cancer cell lines viz., A549 (human lung cancer), HCT116 (human colorectal cancer), HeLa (human cervical cancer), MCF-7, and MDA-MB-231 (human breast cancer).

Materials and Methods: The cells were treated with crude methanolic extract at five different concentrations viz., 25, 50, 100, 250 and 500 µg/ml to assess the cell viability.

Results: The results from the assay performed for five cell lines have shown that methanolic extract of O. dioica has an exponential cytotoxicity potential on HeLa cell line followed by MCF-7 (IC50 value 81.05), HCT116 (106.86) and MDA-MB-231 with IC50 value 81.05, 106.86, 141.34 and 499.24 µg/ml respectively, whereas the A549-human lung cancer cell has shown no cytotoxic activity.

Conclusion: The methanol extract of the O. dioica leaf could be recognized as a pivotal source of anticancer compounds, but furthermore research is needed to investigate the chemical components of O. dioica extracts that are mainly accountable for anticancer action and to expound more precise molecular processes of cell death.
High-performance liquid chromatography method development and validation for the quantification of mangiferin in Coffea arabica leaves
Authors: L.S. Hallur Raghavendra , K. Shaikh Faiyaz , V. N. Magare , Kekuda T. R. Prashith , D. M. Chethan , H. S. Anil Kumar , Shantaram Manjula 
Published: February 2023

Introduction and Aim: Mangiferin is accompanied by the therapeutic potential for various human ailments. The present study is aimed to develop a validated HPLC method for the quantification of mangiferin in coffee (Coffea arabica) leaves.

Materials and Methods: The ­­high-performance liquid chromatography (HPLC) was carried out on Agilent Technologies 1260 LC Infinity Series liquid chromatography system (Agilent, USA) using an isocratic mobile phase of a mixture of methanol and 1% orthophosphoric acid v/v (70:30) at a flow rate 1mL min-1 for 14 min with the controlled temperature at 25°C. The mangiferin content was detected by a UV diode array detector at 258 nm.

Results: The linearity of the HPLC system was established in the range of 10-100 ppm mL-1 with a regression coefficient (R2) of 0.99670. The accuracy of the method was assessed by a study performed using 3 different levels, with a 99% average recovery. The limit of detection(LOD) and limit of quantification (LOQ) were 1.3 and 3.9 ppm mL-1. Intraday and interday precision analysis showed the relative standard deviation 0.012342 separately. The content of mangiferin in the coffee leaves was 94 mg/gram (w/w).

Conclusion: All necessary factors, such as linearity, accuracy, LOD, LOQ, precision, and % relative standard deviation(RSD), were inside the appropriate limits. The HPLC was validated using all necessary variables with a successful estimation of mangiferin.
Knowledge, attitude and perception about surrogacy among urban working women
Authors: Sultana Qudusia , Vaswani Ravi , Vaswani Vina 
Published: February 2023

Introduction and Aim: Infertility is a social stigma in India. Infertility affects 10% of the population in India. Surrogacy is an advanced form of assisted reproductive technique (ART) wherein one woman bears a baby for another woman. Surrogacy is an alternative for the infertile parents for whom it is difficult to have a baby due to medical reasons. The aim was to assess the knowledge, attitude, and perception about the ethical issues involved in surrogacy among urban working women.
Methods: A self-administered questionnaire was devised to determine knowledge, attitude, and perception about surrogacy among young working women. Questionnaire was sent for validation and then distributed to urban working women between the age group of 25-35 years. The questionnaire consisted of 21 questions with a range of 4-point response options and 1 open-ended question. Based on their response given a descriptive analysis was done using SPSS 16.
Results: The urban women were aware of the ethical principles. 63% believe women should not be forced and have complete autonomy in making decisions. 60% believed Surrogate mothers and the baby born of surrogacy should not be ill-treated by the community and 70% said they should be given respect like any other human being.
Conclusion: Altruistic surrogacy should be promoted and not commercial surrogacy as commercial surrogacy leads to the exploitation of the mother’s womb for money. 70% of women in our study believe that the Indian government should not promote surrogacy and in order to protect the rights and prevent injustice suitable Laws should be made and put into action.
Graded repetitive arm supplementary programme (GRASP) and virtual reality to improve upper limb function in patients with acute stroke - An experimental study
Authors: S. Dhanusia , V. Rajalaxmi , N. Suriya , Kumar Bharath 
Published: February 2023

Introduction and Aim: The objective of the study is to improve upper limb function in patients with acute stroke using graded repetitive arm supplementary program (GRASP) and virtual reality. Stroke is described as an sudden neurological outburst brought on by impeded blood flow via the brain’s blood vessels. Stroke has an annual mortality rate of 5.5 million which is the 2nd leading cause of mortality.
Materials and Methods: Fourteen patients were randomly split into two groups, Group A and Group B in A.C.S Medical College and Hospital. Inclusion criteria were persons with first ever acute stroke confirmed by CT or MRI, modified Ashworth scale less than 2, Fugl- Meyer score between 10 to 57, age > 18, both genders, eager participants who are willing volunteers for the study. Exclusion criteria were who were mentally unstable, other neurological conditions, any orthopaedic conditions, modified Ashworth scale more than 2, Fugl Meyer score less than 10 or more than 57, age group < 18, patients with TIA were excluded. Group A trained with GRASP program. Group B trained with virtual reality. Both groups consist of 7 patients and training given for 8 weeks, 60 minutes per day for 6 days a week.
Results: This study demonstrates a statistically significant difference between group A and group B in the hand-held dynamometer, Fugl-Meyer evaluation upper extremity, action research arm test, and EMG with a p value of 0.001. However, when compared to group B, group A exhibits greater relevance across the board.
Conclusion: The study concludes that graded repetitive arm supplementary programme in acute stroke patients demonstrates good recovery in upper limb function.
WORKPLACE TOXICITY AND EMPLOYEE PERFORMANCE OF MANUFACTURING FIRMS: EVIDENCE FROM NIGERIA
Authors: Damaro Olusoji ARUBAYI  
Published: February 2023

Previous studies on work place toxicity has mostly focused on employee satisfaction as well as organizational performance. However, the relationship between Workplace toxicity variables and employee performance has not been established. Consequently, this study assesses the relationship between the variables of workplace toxicity such as narcissistic behaviour, harassment, offensive/aggressive leadership, bullying as well as ostracism and employee performance. This study bridged such gap that may be summarized as follows: there is relationship between narcissistic behaviour, harassment, offensive/aggressive leadership, ostracism, bullying and employee performance. Participants were drawn from selected water production factories operating in the Niger-Delta Region of Nigeria. A structured questionnaire was administered to one hundred and thirty-two respondents. The Pearson Product Moment Correlation results indicate that that there is a relationship between all the variables of Workplace toxicity and Employee Performance. The study recommends, there should be zero harassment policies implemented in the workplace and bullying in the workplace should not be tolerated and victims should be encouraged to speak up via a safe medium.
Keywords: Workplace, Toxicity, Narcissism, Harassment, Offensive/Aggressive, Ostracism,
Bullying, Employee Performance.
WORK-LIFE BALANCE PRACTICES AND EMPLOYEES’ PERFORMANCE: A CORRELATIONAL SURVEY OF COMMERCIAL BANKS IN DELTA AND BAYELSA STATES, NIGERIA
Authors: Odita Anthony Ogomegbunam  
Published: February 2023

This study investigated work-life balance and employees’ performance in some selected commercial banks in Delta and Bayelsa States, Nigeria. The correlational type of survey research design was adopted and the study’s population comprised all the employees of commercial banks with presence in Delta and Bayelsa States out of which seven were selected. 323 employees from the seven selected banks with branches in Abraka, Delta State and Yenizwe Gene, Bayelsa State formed the study’s sample. This research was successfully carried out and completed with the aid of a self-structured questionnaire. 323 copies were personally administered by the researcher to the target respondents and 229 copies were properly filled, scored, and found usable for analysis. The simple percentage, frequencies, charts, mean and standard deviation were used in presenting the respondents’ data on the research items while the Pearson Correlation was adopted for testing the formulated hypotheses. It was discovered that the work leave initiatives practiced among the banks concerns maternity, marriage; annual type, and sick leave. It was also found out that the extent to which the employees give and receive informal support at the workplace is high but the extent to which flexible work arrangements are adopted and wellness programs are organized was low. However, the results of the tested hypotheses indicated that no significant correlation exists between work leave initiatives, informal support practice, and employee performance while a statistically significant relationship was found between flexible work arrangements, wellness programs and employee performance. The researcher drew conclusions from these findings and recommended that adequate wellness schemes such as health insurance (HIS) should be set up by the management of commercial banks for every worker and little deductions be taken monthly from their remunerations in order to ensure that employees’ health are constantly monitored and checkmated by them.
Keywords: Work-Life, Balance Practices, Delta and Bayelsa States, Health Insurance,
Employees’ Performance.
TUJUAN DAN URGENSI PENELITIAN HADIS
Authors: Zakiyah Ulfah , Ghifari Muhammad 
Published: February 2023

Artikel ini menjelakan tentang pentingnya melakukan penelitian hadis yang secara khusus menjelaskan tentang tujuan dan urgensinya. Penelitian hadis merupakan kajian yang mendalam dan kritis terhadap matan dan sanad hadis yang bersifat dinamis dengan menggunakan metode-metode tertentu untuk menentukan validitas hadis sebagai suatu hujjah bagi kebenaran ajaran Islam. Permasalahan pokok dalam penelitian ini adalah bagaimana urgensi penelitian sanad dan matan hadis sebagai unsur penting untuk menentukan kualitas suatu hadis? Untuk menjawap pertanyaan tersebut maka penelitian ini menggunakan jenis penelitian kepustakaan atau yang dikenal dengan istilah library research. Dari hasil penelitian yang dilakukan maka penelitian ini menyimpulkan bahwa da beberapa urgensi hukum yang mendasari pentingnya penelitian hadits, yakni: pertama, terkait dengan posisi hadits sebagai sumber hukum Islam. kedua, terkait dengan historisitas hadits.
Telaah atas Pemikiran T.M. Hasbi Ash-Shiddieqy
Authors: Aziza Ira Nur 
Published: February 2023

Penelitian ini bertujuan untuk mengeksplorasi metode pemahaman hadis T.M. Hasbi Ash-Shiddieqy dan upayanya dalam mengembangkan kajian pemahaman hadis di Indonesia. Penelitian ini mendukung pendapat yang menyatakan bahwa kajian terhadap metode pemahaman hadis di Indonesia telah sampai pada perkembangan yang dinamis. Hal tersebut tercermin dari usaha para pengkaji hadis di Indonesia dalam merekonstruksi metodologi pemahaman hadis agar dapat menyesuaikan dengan kondisi zaman sehingga lebih dapat diterima oleh masyarakat. T.M. Hasbi Ash-Shiddieqy menjadi salah seorang ulama yang mengambil peran dalam bidang ini, yaitu dengan menerapkan metode pemahaman kontekstual dalam menjelaskan hadis-hadis Nabi Saw. Karakteristik yang melekat pada karya-karya hadis T.M. Hasbi Ash-Shiddieqy juga merupakan bukti bahwa perkembangan kajian terhadap metode pemahaman hadis di Indonesia adalah benar adanya. Pembacaan terhadap karya T.M. Hasbi Ash-Shiddieqy pada bidang hadis menunjukkan bahwa dalam memahami hadis ia menggunakan beberapa metode, yaitu memahami hadis dengan petunjuk al-Qur’an, mentakwil hadis-hadis musykīl, mengkompromikan hadis-hadis yang bertentangan, merujuk pada sejumlah referensi dan menggunakan beberapa pendekatan yang meliputi pendekatan historis, bahasa dan kesehatan. Adapun upaya Hasbi dalam mengembangkan kajian hadis di Indonesia tergambar dari langkahnya untuk menyajikan karya syarah hadis berbahasa lokal disaat hal tersebut masih jarang ditemui pada awal sampai pertengahan abad ke-20 karena ulama-ulama yang hidup sebelum Hasbi menulis kitab syarah hadis dalam bahasa Melayu dan Arab