bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomarkers Identification of Hepatocellular Carcinoma Based on Multiomics
Data Integration and Graph-embedded Deep Neural Network
Authors: Yan Chaokun , Li Mengyuan , Suo Zhihao , Zhang Jun , Wang Jianlin , Zhang Ge , Liang Wenjuan , Luo Huimin 
Published: February 2023

Background:
Hepatocellular carcinoma (HCC) is one of the malignancies with high mortality rate, and identify relevant biomarkers of HCC is helpful for early diagnosis and patient care. Though some high-dimensional omic data contains intrinsic biomedical information about HCC, how to integrate analysis them effectively and find promising biomarkers of HCC is still an important and difficult issue.

Methods:
We present a novel biomarker identification approach, named GEDNN, based on multi-omic
data and graph-embedded deep neural network. To achieve a more comprehensive understanding of
HCC, we first collected and normalized the three following types of HCC-related data: DNA methylation,
copy number variation (CNV), and gene expression. The ANOVA was adopted to filter out redundant
genes. Then, we measured the connectivity between gene pairs by Pearson correlation coefficient
of gene pairs, and further construct gene graph. Next, graph-embedded feedforward neural network
(DFN) and back-propagation of convolutional neural network (CNN) were combined to integratively
analyze the three types of omics data and achieve the importance score of gene biomarkers

Results:
Extensive experimental results showed that the biomarkers screened by the proposed method
were effective in classifying and predicting HCC. Furthermore, the gene analysis further showed that
the biomarkers screened by our method were strongly associated with the development of HCC.

Conclusion:
In this paper, we propose the GEDNN method to assess the importance of genes for more
accurate identification of cancer biomarkers, which facilitates the effective classification of cancers. The
proposed method is applied to multi-omics data of HCC, including RNASeq, DNAMeth and CNV, considering
the complementary information between different types of data. We construct a gene graph by
Pearson correlation coefficients as additional information for DFN, thus reducing the importance score
of redundant genes. In addition, the proposed method also incorporates back-propagation of CNN to
further obtain the importance of features.
Medicinal, Pharmacological and Biochemical Progress on the Study of
Genus Helianthemum: A Review
Authors: Mouffouk Soumia , Mouffouk Chaima , Mouffouk Sara , Haba Hamada 
Published: February 2023

Abstract:
The genus Helianthemum from the Cistaceae family includes about 110 plants, which are
well known for their wide therapeutic uses and interesting pharmacological activities. To the best of
our knowledge, there are no previous comprehensive and critical reviews on the therapeutic applications,
chemical composition, and biological properties of this genus. In this context, the present paper
describes a systematic mini-review on the traditional use, phytochemical composition, and pharmacological
properties of species belonging to the genus Helianthemum. For this, more than 75 papers covering
the period from 1970 to 2022 were collected and analyzed to achieve this review. From 110 species
of the genus Helianthemum, only 22 species were studied. Helianthemum species are widely used
in folk medicine for the treatment of respiratory, hepatic, renal, psychiatric, and inflammatory diseases.
According to the collected information, species from this genus have several pharmacological
properties linked to their rich chemical composition, particularly in phenolic compounds and flavonoids.
In fact, Helianthemum plants have antioxidant, antimicrobial, anti-inflammatory, analgesic, antidiabetic,
insecticide, anticancer, antiulcer, larvicide, chemo, and hepatoprotective activities, justifying
their various uses in traditional medicine. Despite the intense use of Helianthemum species in traditional
human and veterinary medicine, few studies were conducted on the phytochemical composition
and pharmacological activities of the plants constituting this genus. In fact, until now, the emphasis
has only been on twenty-two species and the work carried out was not explanatory, especially for
certain species. So it will be interesting to broaden the spectrum of the tested biological activities, especially
for endemic species in order to understand the possible modes of action and to confirm the
toxicity of certain species.
Gonolobus gonzaleziarum (Apocynaceae), especie nueva de la ecorregión Madrense Tropical de la Sierra Madre Occidental, México
Authors: Pío-León Juan F. , Castro-Castro Arturo , Alvarado-Cárdenas Leonardo O. 
Published: February 2023

Antecedentes y Objetivos: Gonolobus comprende alrededor de 150 especies en el mundo, con poco más de 40 de ellas distribuidas en México. En los últimos dos años se han descrito al menos cinco nuevas especies de este país. En el presente trabajo se describe una nueva especie dentro del subgénero Pseudolachnostoma.
Métodos: Se revisaron 13 herbarios, tres bases de datos digitales y literatura especializada. Con base en esto se compararon las especies similares y se generó una clave de identificación. Se realizaron expediciones a campo de 2019 a 2022 y se colectó y monitoreó la fenología de la especie de interés. Se planteó como hipótesis explicativa el concepto cohesivo de especie para el reconocimiento de los taxones aquí tratados. Resultados clave: Se describe la especie nueva Gonolobus gonzaleriarum, conocida de dos localidades de bosques de Pinus-Quercus entre los límites sureste y suroeste de los estados de Sinaloa y Durango, respectivamente. Se diferencia de las especies del subgénero Pseudolachnostoma por poseer ginostegio con un estípite reducido, lóbulos de la corola y sépalos de menor longitud que el tubo, aroma cítrico e inflorescencia densa. Se incluyen láminas fotográficas y una ilustración con los caracteres distintivos de la nueva especie, así como una clave dicotómica con las especies del subgénero Pseudolachnostoma. Con base en su área de distribución reducida y hábitat impactado, se considera una especie amenazada de acuerdo con los criterios de la IUCN.Conclusiones: Con el presente descubrimiento, el género Gonolobus llega a 45 especies en México con 24 endémicas. Gonolobus gonzaleriarum se considera con gran potencial ornamental por sus inflorescencias conspicuas y agradable aroma cítrico, lo que motiva en fomentar estrategias para su conservación, ya que se encuentra en una distribución restringida y hábitat amenazado.
Using Social Media Data to Investigate Public Perceptions of Cannabis as a Medicine: Narrative Review
Authors: Khademi Sedigh , Hallinan Christine Mary , Conway Mike , Bonomo Yvonne 
Published: February 2023

Background
The use and acceptance of medicinal cannabis is on the rise across the globe. To support the interests of public health, evidence relating to its use, effects, and safety is required to match this community demand. Web-based user-generated data are often used by researchers and public health organizations for the investigation of consumer perceptions, market forces, population behaviors, and for pharmacoepidemiology.

Objective
In this review, we aimed to summarize the findings of studies that have used user-generated text as a data source to study medicinal cannabis or the use of cannabis as medicine. Our objectives were to categorize the insights provided by social media research on cannabis as medicine and describe the role of social media for consumers using medicinal cannabis.

Methods
The inclusion criteria for this review were primary research studies and reviews that reported on the analysis of web-based user-generated content on cannabis as medicine. The MEDLINE, Scopus, Web of Science, and Embase databases were searched from January 1974 to April 2022.

Results
We examined 42 studies published in English and found that consumers value their ability to exchange experiences on the web and tend to rely on web-based information sources. Cannabis discussions have portrayed the substance as a safe and natural medicine to help with many health conditions including cancer, sleep disorders, chronic pain, opioid use disorders, headaches, asthma, bowel disease, anxiety, depression, and posttraumatic stress disorder. These discussions provide a rich resource for researchers to investigate medicinal cannabis–related consumer sentiment and experiences, including the opportunity to monitor cannabis effects and adverse events, given the anecdotal and often biased nature of the information is properly accounted for.

Conclusions
The extensive web-based presence of the cannabis industry coupled with the conversational nature of social media discourse results in rich but potentially biased information that is often not well-supported by scientific evidence. This review summarizes what social media is saying about the medicinal use of cannabis and discusses the challenges faced by health governance agencies and professionals to make use of web-based resources to both learn from medicinal cannabis users and provide factual, timely, and reliable evidence-based health information to consumers.
A Web Tool to Help Counter the Spread of Misinformation and Fake News: Pre-Post Study Among Medical Students to Increase Digital Health Literacy
Authors: Moretti Valentina , Brunelli Laura , Conte Alessandro , Valdi Giulia , Guelfi Maria Renza , Masoni Marco , Anelli Filippo , Arnoldo Luca 
Published: February 2023

Background
The COVID-19 pandemic was accompanied by the spread of uncontrolled health information and fake news, which also quickly became an infodemic. Emergency communication is a challenge for public health institutions to engage the public during disease outbreaks. Health professionals need a high level of digital health literacy (DHL) to cope with difficulties; therefore, efforts should be made to address this issue starting from undergraduate medical students.

Objective
The aim of this study was to investigate the DHL skills of Italian medical students and the effectiveness of an informatics course offered by the University of Florence (Italy). This course focuses on assessing the quality of medical information using the “dottoremaeveroche” (DMEVC) web resource offered by the Italian National Federation of Orders of Surgeons and Dentists, and on health information management.

Methods
A pre-post study was conducted at the University of Florence between November and December 2020. First-year medical students participated in a web-based survey before and after attending the informatics course. The DHL level was self-assessed using the eHealth Literacy Scale for Italy (IT-eHEALS) tool and questions about the features and quality of the resources. All responses were rated on a 5-point Likert scale. Change in the perception of skills was assessed using the Wilcoxon test.

Results
A total of 341 students participated in the survey at the beginning of the informatics course (women: n=211, 61.9%; mean age 19.8, SD 2.0) and 217 of them (64.2%) completed the survey at the end of the course. At the first assessment, the DHL level was moderate, with a mean total score of the IT-eHEALS of 2.9 (SD 0.9). Students felt confident about finding health-related information on the internet (mean score of 3.4, SD 1.1), whereas they doubted the usefulness of the information they received (mean score of 2.0, SD 1.0). All scores improved significantly in the second assessment. The overall mean score of the IT-eHEALS significantly increased (P<.001) to 4.2 (SD 0.6). The item with the highest score related to recognizing the quality of health information (mean score of 4.5, SD 0.7), whereas confidence in the practical application of the information received remained the lowest (mean of 3.7, SD 1.1) despite improvement. Almost all students (94.5%) valued the DMEVC as an educational tool.

Conclusions
The DMEVC tool was effective in improving medical students’ DHL skills. Effective tools and resources such as the DMEVC website should be used in public health communication to facilitate access to validated evidence and understanding of health recommendations.
Postmarketing Follow-Up of a Digital Home Exercise Program for Back, Hip, and Knee Pain: Retrospective Observational Study With a Time-Series and Matched-Pair Analysis
Authors: Teepe Gisbert Wilhelm , Kowatsch Tobias , Hans Felix Patricius , Benning Leo 
Published: February 2023

Background
Musculoskeletal conditions are the main drivers of global disease burden and cause significant direct and indirect health care costs. Digital health applications improve the availability of and access to adequate care. The German health care system established a pathway for the approval of “Digitale Gesundheitsanwendungen” (DiGAs; Digital Health Applications) as collectively funded medical services through the “Digitale-Versorgung-Gesetz” (Digital Health Care Act) in 2019.

Objective
This article presents real-world prescription data collected through the smartphone-based home exercise program “Vivira,” a fully approved DiGA, regarding its effect on self-reported pain intensity and physical inability in patients with unspecific and degenerative pain in the back, hip, and knee.

Methods
This study included 3629 patients (71.8% [2607/3629] female; mean age 47 years, SD 14.2 years). The primary outcome was the self-reported pain score, which was assessed with a verbal numerical rating scale. The secondary outcomes were self-reported function scores. To analyze the primary outcome, we used a 2-sided Skillings-Mack test. For function scores, a time analysis was not feasible; therefore, we calculated matched pairs using the Wilcoxon signed-rank test.

Results
Our results showed significant reductions in self-reported pain intensity after 2, 4, 8, and 12 weeks in the Skillings-Mack test (T3628=5308; P<.001). The changes were within the range of a clinically relevant improvement. Function scores showed a generally positive yet more variable response across the pain areas (back, hip, and knee).

Conclusions
This study presents postmarketing observational data from one of the first DiGAs for unspecific and degenerative musculoskeletal pain. We noted significant improvements in self-reported pain intensity throughout the observation period of 12 weeks, which reached clinical relevance. Additionally, we identified a complex response pattern of the function scores assessed. Lastly, we highlighted the challenges of relevant attrition at follow-up and the potential opportunities for evaluating digital health applications. Although our findings do not have confirmatory power, they illustrate the potential benefits of digital health applications to improve the availability of and access to medical care.

Trial Registration
German Clinical Trials Register DRKS00024051; https://drks.de/search/en/trial/DRKS00024051
Assessing the Quality and Impact of eHealth Tools: Systematic Literature Review and Narrative Synthesis
Authors: Jacob Christine , Lindeque Johan , Klein Alexander , Ivory Chris , Heuss Sabina , Peter Marc K 
Published: February 2023

Background
Technological advancements have opened the path for many technology providers to easily develop and introduce eHealth tools to the public. The use of these tools is increasingly recognized as a critical quality driver in health care; however, choosing a quality tool from the myriad of tools available for a specific health need does not come without challenges.

Objective
This review aimed to systematically investigate the literature to understand the different approaches and criteria used to assess the quality and impact of eHealth tools by considering sociotechnical factors (from technical, social, and organizational perspectives).

Methods
A structured search was completed following the participants, intervention, comparators, and outcomes framework. We searched the PubMed, Cochrane, Web of Science, Scopus, and ProQuest databases for studies published between January 2012 and January 2022 in English, which yielded 675 results, of which 40 (5.9%) studies met the inclusion criteria. The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines and the Cochrane Handbook for Systematic Reviews of Interventions were followed to ensure a systematic process. Extracted data were analyzed using NVivo (QSR International), with a thematic analysis and narrative synthesis of emergent themes.

Results
Similar measures from the different papers, frameworks, and initiatives were aggregated into 36 unique criteria grouped into 13 clusters. Using the sociotechnical approach, we classified the relevant criteria into technical, social, and organizational assessment criteria. Technical assessment criteria were grouped into 5 clusters: technical aspects, functionality, content, data management, and design. Social assessment criteria were grouped into 4 clusters: human centricity, health outcomes, visible popularity metrics, and social aspects. Organizational assessment criteria were grouped into 4 clusters: sustainability and scalability, health care organization, health care context, and developer.

Conclusions
This review builds on the growing body of research that investigates the criteria used to assess the quality and impact of eHealth tools and highlights the complexity and challenges facing these initiatives. It demonstrates that there is no single framework that is used uniformly to assess the quality and impact of eHealth tools. It also highlights the need for a more comprehensive approach that balances the social, organizational, and technical assessment criteria in a way that reflects the complexity and interdependence of the health care ecosystem and is aligned with the factors affecting users’ adoption to ensure uptake and adherence in the long term.
The effects of replacing fishmeal by mealworm (Tenebrio molitor) on digestive enzymes activity and hepatopancreatic biochemical indices of Litopenaeus vannamei
Authors: Sharifinia Moslem , Bahmanbeigloo Zahra Afshari , Keshavarzifard Mehrzad , Khanjani Mohammad Hossein , Daliri Moslem , Koochaknejad Emad , Jasour Mohammad Sedigh 
Published: February 2023

Abstract
Reducing the use of fishmeal (FM) in aquafeed means a significant saving in the amount of FM at the global level and reducing environmental impacts. One of the potential protein sources to replace FM in shrimp diet is the use of insects’ meal. Therefore, the present study aimed to examine the effects of replacing FM with mealworm (MW; Tenebrio molitor) on the growth, digestive enzymes activity and hepatopancreatic biochemical indices of Pacific white shrimp (Litopenaeus vannamei). Shrimp were fed for 60 days with a control diet (T0) and 4 practical diets (T15, T30, T60 and T100) where 15, 30, 60 and 100% of the FM was substituted by MW, respectively. Results showed that there were significant differences in weight gain (WG) and hepatopancreatic index (HPI) among treatments and the lowest and highest values for were observed in T0 and T30, respectively. Our findings indicated a significant increase (P<0.05) in activity of glutathione peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD), total nitric oxide (TNO) and total antioxidant capacity (TAC) and a meaningful decrease (P<0.05) of malondialdehyde (MDA) in hepatopancreas of L. vannamei juveniles fed diets containing MW. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the T0 did not show a significant difference (P>0.05) with other experimental treatments. Protease and lipase indicated an increasing trend with increasing the amount of MW up to 60%. The protease activity showed a significant difference (P<0.05) between the treatments containing MW and the control treatment. These findings indicated that MW could be a feasible candidate for replacing FM in diets of the Pacific white shrimp without any detrimental effects.
Capric and lauric acid mixture decreased rumen methane production, while combination with nitrate had no further benefit in methane reduction
Authors: Joch Miroslav , Vadroňová Mariana , Češpiva Miroslav , Zabloudilová Petra , Výborná Alena , Tyrolová Yvona , Kudrna Václav , Tichá Denisa , Plachý Vladimír , Hroncová Zuzana 
Published: February 2023

Abstract
This study aimed to evaluate the methane-reducing potential of individual and combined treatments of low levels of nitrate (NIT) and a mixture of capric/lauric acid (CL) in dairy cows. Both in vitro and in vivo experiments were conducted. In the in vitro experiment, the anti-methanogenic effects of NIT (1.825 mmol/l) and CL (250 mg/l; capric acid, 125 mg/l + lauric acid, 125 mg/l) were evaluated in a 2 × 2 factorial design using consecutive batch incubations with rumen fluid. The NIT and CL reduced (P<0.05) methane production by 9.2% and by 21.3%, respectively. However, combining NIT with CL did not show (P>0.05) any benefit in methane reduction compared to the use of CL alone. In the in vivo experiment, eight multiparous dry Holstein cows were fed two diets in a crossover design for two 21-day periods (14 days of adaptation and 7 days of sampling). The treatments were: 1) silage-based basal diet + 100 g stearic acid per cow/d (CON) and 2) silage-based basal diet + 50 g capric acid + 50 g lauric acid per cow/d (CL). Gas emissions were measured using open-circuit respiration chambers. Methane production (g/d) was reduced (by 11.5%; P = 0.012) when the diet was supplemented with CL. However, supplementation with CL increased ruminal ammonia-N concentration (by 28.5%; P = 0.015) and gas ammonia production (g/d; by 37.2%; P = 0.005). Ruminal pH, protozoa count, and total and individual volatile fatty acid concentrations (VFA ) did not differ (P>0.05) between the treatments. Treatment did not affect the intake and apparent total tract digestibility (P>0.05). In conclusion, our results suggest that low CL levels have anti-methanogenic potential. However, low levels of CL may compromise nitrogen use efficiency.
Meat quality, metabolic profile and antioxidant status of lambs fed on seedless grape pomace (Vitis vinifera L.)
Authors: Antunović Zvonko , Šalavardić Željka Klir , Steiner Zvonimir , Đidara Mislav , Drenjančević Mato , Ronta Mario , Pavić Valentina , Barron Lidija Jakobek , Novoselec Josip 
Published: February 2023

Abstract
This study aimed to research the effect of the seedless grape pomace (GP) added to feed mixture on meat quality, metabolic profile, and antioxidant status of Merinolandschaf lambs. The 90-day-old lambs in the control group (C, n=10) were fed feed mixture without GP while in experimental groups they were fed with 10% (GP10, n=10) or 20% (GP20, n=10) of GP in feed mixture for 30 days. There was a significant increase in L*, a* values and hue angle as well as a decrease in b* values of lamb carcasses in GP10 and GP20 compared to C group. When lambs were fed GP glucose concentrations decreased, while non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB) increased on the 30th day in GP10 and GP20 compared with C group. A significant increase in SOD activity on day 30 and GPx activity on day 15 in lambs’ blood of GP10 and GP20 compared with C group was determined. Also, an increase in DPPH in lamb GP10 and GP20 compared with C was determined. The obtained results of the meat quality, metabolic profile and antioxidant status parameters of lamb meat showed that the use of 10% GP in the diet is justified, while 20% of GP was questionable.
Total and partial magnetic retained overdenture. A clinical report.
Authors: Bogdan-Andreescu Claudia Florina , Bănățeanu Andreea Mariana , Botoacă Oana , Rădulescu Diana Eugenia 
Published: February 2023

Abstract
An overdenture is a denture supported partly by soft tissues and partly by retained teeth or implants. Magnetic tooth-retained overdentures are used to increase support, retention, and stability but also prevent alveolar ridge resorption. Magnetic attachments increase the retention of partial or complete dentures regardless of the path of the insertion and, in addition, can be used for abutment teeth with periodontal disease. This paper discusses two oral rehabilitation cases with total and partial tooth-retained overdenture.