bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing aerobic physical efficiency through temple surface temperature measurements during light, heavy exercise, and recovery
Authors: Jastrzębska Agnieszka Danuta , Hebisz Rafał , Hebisz Paulina 
Published: September 2023

AbstractThe study was conducted to determine thecorrelation between the selected measures of aerobic physical efficiency and changes in the temple surface temperature in response to light and heavy exercise. 25 physically active men aged 19–25 were recruited for the study. They performed a graded exercise test on a cycle ergometer to measure maximum power (Pmax) and a test verifying the value of maximum oxygen uptake (VO2max). Then, two 3-min submaximal efforts with constant-intensity of 2.2 W·kgLBM−1 and 5 W·kgLBM−1, respectively were performed. During the constant-intensity efforts, the temperature of the temple surface was measured. Then, the difference between the temperature of the temple measured at the end of the exercise and the temperature measured at the beginning of the exercise was calculated (ΔT1-2.2, ΔT1-5, respectively). It was shown that ΔT1-2.2 correlated statistically significantly with VO2max (ml·min−1·kg−1) (r = 0.49; p = 0.01) and Pmax (W·kg−1) (r = 0.41, p = 0.04). Moreover, ΔT1-5 correlated statistically significantly with VO2max (l·min−1) (r = − 0.41; p = 0.04). Changes in body surface temperature in response to light exercise positively correlate with measurements of aerobic physical efficiency, such as VO2max and Pmax. When the exercise intensity is high (5 W·kgLBM−1), the correlation between exercise body temperature changes and VO2max becomes negative.
High biosorption of cationic dye onto a novel material based on paper mill sludge
Authors: Merah Meriem , Boudoukha Chahra , Avalos Ramirez Antonio , Haroun Mohamed Fahim , Maane Samira 
Published: September 2023

AbstractThe valorization of paper mill sludge (PMS) is the main goal of this study. The emissions of PMS continue to increase at global scale, especially from packaging paper and board sectors. The raw sludge was used to prepare an adsorbent to remove toxic pollutants from wastewater, the methylene blue (MB), an organic dye. Firstly, the physico-chemical characterization of PMS was done determining the crystalline phases of PMS fibers, the content of main elements, and the pH zero point charge, which was determined at around pH 7. The adsorption of MB on PMS powder was studied at 18 °C with an agitation of 200 rpm, being the best operating conditions 30 min of contact time, 250 mg L−1 of initial MB concentration and 0.05 g in 25 mL of adsorbent dose. Experimental data of MB adsorption was fitted to Langmuir and Freundlich isotherm equations. The Langmuir model was more accurate for the equilibrium data of MB adsorption at pH 5.1. The PFOM and PSOM were adjusted to experimental adsorption kinetics data, being PSOM, which describes better the MB adsorption by PMS powder. This was confirmed by calculating the maximum adsorption capacity with PSOM, which was 42.7 mg g−1, being nearly similar of the experimental value of 43.5 mg g−1. The analysis of adsorption thermodynamics showed that the MB was adsorbed exothermically with a ΔH0 = − 20.78 kJ mol−1, and spontaneously with ΔG0 from − 0.99 to − 6.38 kJ mol−1 in the range of temperature from 291 to 363 K, respectively. These results confirm that the sludge from paper industry can be used as biosorbent with remarkable adsorption capacity and low cost for the treatment of wastewater. PMS can be applied in the future for the depollution of the effluents from the textile industry, which are highly charged with dyes.
Correlation between the presence of a cecal appendix and reduced diarrhea severity in primates: new insights into the presumed function of the appendix
Authors: Collard Maxime K. , Bardin Jérémie , Marquet Bertille , Laurin Michel , Ogier-Denis Éric 
Published: September 2023

AbstractIncreased severity or recurrence risk of some specific infectious diarrhea, such a salmonellosis or Clostridium difficile colitis, have been reported after an appendectomy in human patients. While several other mammals also possess an appendix, the suspected protective function against diarrhea conferred by this structure is known only in humans. From a retrospective collection of veterinary records of 1251 primates attributed to 45 species, including 13 species with an appendix and 32 without, we identified 2855 episodes of diarrhea, 13% of which were classified as severe diarrhea requiring a therapeutic medication or associated with a fatal issue. We identified a lower risk of severe diarrhea among primate species with an appendix, especially in the early part of life when the risk of diarrhea is maximal. Moreover, we observed a delayed onset of diarrhea and of severe diarrhea in species possessing an appendix. Interestingly, none of the primates with an appendix were diagnosed, treated or died of an acute appendicitis during the 20 years of veterinarian follow-up. These results clarify the function of the appendix among primates, as protection against diarrhea. This supports its presumed function in humans and is congruent with the existence of a selective advantage conferred by this structure.
Attenuation properties of hybrid nanocomposite film containing Ce2O, GO, and α-Al2O3 nanoparticles for high energy radiations
Authors: Mahmoudian Mehdi , Radmehr Mahsa , Alimoradlou Khadijeh , Zamani Asghar , Balkanloo Peyman Gozali 
Published: September 2023

AbstractThe use of diagnostic radiation in medical centers has spread due to the incidence of various diseases. Thus, it is essential that patients and medical staff wear protective clothing to protect themselves from their harmful effects. In the past, lead protective clothing has been used; however, the toxicity and heaviness of lead have limited the tendency to use these clothing. Recently, nanocomposites containing heavy element nanoparticles have been introduced as an alternative to lead coatings. In this study, hybrid nanocomposites containing ceria (CeO2), alumina (Al2O3), and graphene oxide (GO) nanoparticles were studied for this purpose. Ceria, alumina, and graphene oxide nanoparticles were mixed with polyethylenevinylacetate (EVA) dissolved in chloroform and casted on a glass plate to form nanocomposite films. The prepared nanoparticles and films were characterized by Fourier Transform Infrared Spectroscopy, Field Emission Scanning Electron Microscope, Thermal Gravimetric Analysis, and Energy Dispersive X-ray Analysis, and then the attenuation properties of the films against high-energy radiation (120 kV) were studied in two narrow and broad beam geometries. The results showed that hybrid films, despite having a lower percentage of nanoparticles, showed better attenuation properties, which indicated the synergistic effect of nanoparticles with different mechanisms in attenuating the radiations. The attenuation ability of these films was considerable due to their lower density compared to lead. The fabricated hybrid nanocomposite films with a suitable performance in attenuation of high-energy radiations used in therapeutic diagnostics, can be proposed as a suitable alternative to conventional lead clothing.
The social relevance and the temporal constraints of motor resonance in humans
Authors: Guidali Giacomo , Picardi Michela , Franca Maria , Caronni Antonio , Bolognini Nadia 
Published: September 2023

AbstractIn humans, motor resonance effects can be tracked by measuring the enhancement of corticospinal excitability by action observation. Uncovering factors driving motor resonance is crucial for optimizing action observation paradigms in experimental and clinical settings. In the present study, we deepen motor resonance properties for grasping movements. Thirty-five healthy subjects underwent an action observation task presenting right-hand grasping movements differing from their action goal. Single-pulse transcranial magnetic stimulation was applied over the left primary motor cortex at 100, 200, or 300 ms from the onset of the visual stimulus depicting the action. Motor-evoked potentials were recorded from four muscles of the right hand and forearm. Results show a muscle-specific motor resonance effect at 200 ms after movement but selectively for observing a socially relevant grasp towards another human being. This effect correlates with observers’ emotional empathy scores, and it was followed by inhibition of motor resonance at 300 ms post-stimulus onset. No motor resonance facilitation emerged while observing intransitive hand movement or object grasping. This evidence highlights the social side of motor resonance and its dependency on temporal factors.
Shear strength characteristics of basalt fiber-reinforced loess
Authors: Chen Chong-kun , Li Gang , Liu Jia , Xi Yu , Nan Jing-jing 
Published: September 2023

AbstractLoess owns the characteristics of collapsibility, disintegration and solubility, which pose a challenge to engineering construction. To examine the shear strength of basalt fiber-reinforced (BFR) loess, consolidated undrained (CU) triaxial tests were conducted to explore the impacts of water content (w), fiber length (FL), fiber content (FC) and cell pressure (σ3) on the shear strength. According to the results, the shear strength model was established taken into account the impacts of FL, FC, and fiber diameter (d). The results showed that the peak strength of BFR soils enhanced as FL, FC, and σ3 increasing, whereas it decreased with increasing of w. Compared to unreinforced soil, the peak strength of BFR loess improved 64.60% when FC was 0.2% and FL was 16 mm. The optimum reinforcement condition for experimental loess was that of FL was 16 mm and FC was 0.8%. The reinforcing mechanism of fibers was divided into a single tensile effect and spatial mesh effect. The experimental and calculated results agreed well, which suggested the model is suitable for predicting the shear strength of BFR loess. The research results can offer a guideline for the application of BFR loess in the subgrade and slope engineering.
Strong Rashba parameter of two-dimensional electron gas at CaZrO3/SrTiO3 heterointerface
Authors: Kwon Duhyuk , Kwak Yongsu , Lee Doopyo , Jo Wonkeun , Cho Byeong-Gwan , Koo Tae-Yeong , Song Jonghyun 
Published: September 2023

AbstractWe synthesized a CaZrO3/SrTiO3 oxide heterostructure, which can serve as an alternative to LaAlO3/SrTiO3, and confirmed the generation of 2-dimensional electron gas (2-DEG) at the heterointerface. We analyzed the electrical-transport properties of the 2-DEG to elucidate its intrinsic characteristics. Based on the magnetic field dependence of resistance at 2 K, which exhibited Weak Anti-localization (WAL) behaviors, the fitted Rashba parameter values were found to be about 12–15 × 10–12 eV*m. These values are stronger than the previous reported Rashba parameters obtained from the 2-DEGs in other heterostructure systems and several layered 2D materials. The observed strong spin–orbit coupling (SOC) is attributed to the strong internal electric field generated by the lattice mismatch between the CaZrO3 layer and SrTiO3 substrate. This pioneering strong SOC of the 2-DEG at the CaZrO3/SrTiO3 heterointerface may play a pivotal role in the developing future metal oxide-based quantum nanoelectronics devices.
Aortic pulse wave comparison between controls and triathletes in baseline conditions and in response to acute maximum exercise
Authors: Ianê-Siva Camila , Gonçalves Reginaldo , de Oliveira Otoni Claudinéia , Dias Koren C. R. , Stein Ricardo , Rezende Bruno Almeida , Rodrigues-Machado Maria Glória 
Published: September 2023

AbstractTo determine the effects of intense training on aortic pulse wave variables and hemodynamic parameters at baseline and at recovery from maximal exercise testing (MaxET) in triathletes compared with sedentary individuals. In this prospective and experimental study, 21 triathletes and 21 sedentary individuals were recruited and evaluated prior and two minutes after the MaxET using the Mobil-O-Graph®, which estimates the aortic pulse wave from the brachial artery pressure. The augmentation index (AIx@75) was lower in triathletes after the MaxET compared to control group (16.34 ± 5.95 vs. 23.5 ± 8.53%, p = 0.001), while the pulse wave velocity (PWV) was similar between groups. The heart rate was significantly lower at baseline and after MaxET in triathletes group (55.70 ± 8.95 bpm 91.49 ± 11.39 bpm) compared with control group (62.11 ± 6.70 bpm; 102.08 ± 10.85 bpm). The stroke volume was significantly higher at baseline (96.08 ± 13.96 ml; 86.17 ± 11.24 ml) and after MaxET in triathletes group (69.15 ± 6.51 ml, 58.38 ± 6.99 ml) compared with control group. Triathetes show lower value of AIx@75 after MaxET in comparison with the control group. AIx@75, in addition to being an indirect measure of arterial stiffness, is also a measure of left ventricular afterload. Thus, the lower AIx@75 in triathletes may be due to their lower left ventricular afterload, lower myocardial oxygen demand, and greater coronary perfusion than sedentary individuals. The hemodynamic changes observed in triathletes at rest and during an acute exercise bout are distinctive characteristics of aerobic physical training.
Volcanic monitoring of the 2021 La Palma eruption using long-period magnetotelluric data
Authors: Piña-Varas P. , Ledo J. , Queralt P. , van Dorth D. Martínez , Marcuello A. , Cabrera-Pérez I. , D’Auria L. , Martí A. 
Published: September 2023

AbstractBetween September and December 2021, the first subaerial volcanic eruption in the Canary Islands in 50 years took place on the island of La Palma. Since November 2021, we have been conducting a long-period magnetotelluric (MT) monitoring experiment at a site located 2.4 km east of the volcanic cone. Having continuously recorded data since then, the obtained dataset shows significant changes in resistivity over the fourteen months following the eruption: more than ± 20% in apparent resistivity and ± 2 degrees in phase. These temporal variations in electrical resistivity, recorded continuously using long-period MT during both the syn- and post-eruptive stages, have not been reported to date, making this dataset unique. Four estimated impedances have been selected as representatives of the major temporal changes observed and inverted to generate new 3-D resistivity models. The results provide novel key information on the spatiotemporal evolution of the subsoil's electrical resistivity, enabling the characterization of a set of structures acting as preferred magmatic fluid pathways. Therefore, our study highlights the strong potential of MT as a volcanic monitoring tool and provides new insights about the evolution of the fluid pathways during the post-eruptive stage. These findings enhance our understanding of the magmatic system and may contribute to volcanic hazard mitigation in the future.
Urbanization alters atmospheric dryness through land evapotranspiration
Authors: Hao Lu , Sun Ge , Huang Xiaolin , Tang Run , Jin Kailun , Lai Yihan , Chen Dongxu , Zhang Yaoqi , Zhou Decheng , Yang Zong-Liang , Wang Lang , Dong Gang , Li Wenhong 
Published: September 2023

Abstract‘Urban Dry (Wet) Islands’ (UDI/UWI) represents microclimate change that impacts ecosystems and human well-being. However, causes of the UDI (UWI) phenomena are not fully understood due to the lack of empirical data. Here, we quantify UDI (UWI) using global observations of atmospheric humidity, evapotranspiration (ET), and land surface characteristics across 25 large urban agglomerations. We show that UDI (17) and UWI (8) are closely tied to local ET, global warming, and ‘Urban Heat Islands’ through intertwined linkages with water and energy balances. UDI is most pronounced in humid vegetated regions where mean urban-rural annual ET differences are as high as 215 mm, whereas UWI is found in arid regions or in climates with dry summers. We conclude that ET can be used as a single variable to explain emerging urban environmental changes. Our study supports a concerted strategy of restoring nature’s ET power as effective ‘Nature-based Solutions’ to mitigate the negative environmental effects of urbanization.
Distinct aneuploid evolution of astrocytoma and glioblastoma during recurrence
Authors: Zhang Jinsen , Feng Yuan , Li Guanghao , Zhang Jianhua , Zhang Xin , Zhang Yi , Qin Zhiyong , Zhuang Dongxiao , Qiu Tianming , Shi Zhifeng , Zhu Wei , Zhang Rui , Wu Yonghe , Liu Haikun , Cao Dandan , Hua Wei , Mao Ying 
Published: September 2023

AbstractAstrocytoma and glioblastoma (GB) are reclassified subtypes of adult diffuse gliomas based on distinct isocitrate dehydrogenase (IDH) mutation in the fifth edition of the WHO Classification of Tumors of the Central Nervous System. The recurrence of gliomas is a common and inevitable challenge, and analyzing the distinct genomic alterations in astrocytoma and GB could provide insights into their progression. This study conducted a longitudinal investigation, utilizing whole-exome sequencing, on 65 paired primary/recurrent gliomas. It examined chromosome arm aneuploidies, copy number variations (CNVs) of cancer-related genes and pathway enrichments during the relapse. The veracity of these findings was verified through the integration of our data with multiple public resources and by corroborative immunohistochemistry (IHC). The results revealed a greater prevalence of aneuploidy changes and acquired CNVs in recurrent lower grade astrocytoma than in relapsed grade 4 astrocytoma and GB. Larger aneuploidy changes were predictive of an unfavorable prognosis in lower grade astrocytoma (P < 0.05). Further, patients with acquired gains of 1q, 6p or loss of 13q at recurrence had a shorter overall survival in lower grade astrocytoma (P < 0.05); however, these prognostic effects were confined in grade 4 astrocytoma and GB. Moreover, acquired gains of 12 genes (including VEGFA) on 6p during relapse were associated with unfavorable prognosis for lower grade astrocytoma patients. Notably, elevated VEGFA expression during recurrence corresponded to poorer survival, validated through IHC and CGGA data. To summarize, these findings offer valuable insights into the progression of gliomas and have implications for guiding therapeutic approaches during recurrence.
capTEs enables locus-specific dissection of transcriptional outputs from reference and nonreference transposable elements
Authors: Li Xuemei , Lu Keying , Chen Xiao , Tu Kailing , Xie Dan 
Published: September 2023

AbstractTransposable elements (TEs) serve as both insertional mutagens and regulatory elements in cells, and their aberrant activity is increasingly being revealed to contribute to diseases and cancers. However, measuring the transcriptional consequences of nonreference and young TEs at individual loci remains challenging with current methods, primarily due to technical limitations, including short read lengths generated and insufficient coverage in target regions. Here, we introduce a long-read targeted RNA sequencing method, Cas9-assisted profiling TE expression sequencing (capTEs), for quantitative analysis of transcriptional outputs for individual TEs, including transcribed nonreference insertions, noncanonical transcripts from various transcription patterns and their correlations with expression changes in related genes. This method selectively identified TE-containing transcripts and outputted data with up to 90% TE reads, maintaining a comparable data yield to whole-transcriptome sequencing. We applied capTEs to human cancer cells and found that internal and inserted Alu elements may employ distinct regulatory mechanisms to upregulate gene expression. We expect that capTEs will be a critical tool for advancing our understanding of the biological functions of individual TEs at the locus level, revealing their roles as both mutagens and regulators in biological and pathogenic processes.
GiRAFR improves gRNA detection and annotation in single-cell CRISPR screens
Authors: Yu Qian , Van Minsel Paulien , Galle Eva , Thienpont Bernard 
Published: September 2023

AbstractNovel methods that combine single cell RNA-seq with CRISPR screens enable high-throughput characterization of transcriptional changes caused by genetic perturbations. Dedicated software is however lacking to annotate CRISPR guide RNA (gRNA) libraries and associate them with single cell transcriptomes. Here, we describe a CRISPR droplet sequencing (CROP-seq) dataset. During analysis, we observed that the most commonly used method fails to detect mutant gRNAs. We therefore developed a python tool to identify and characterize intact and mutant gRNAs, called GiRAFR. We show that mutant gRNAs are dysfunctional, and failure to detect and annotate them leads to an inflated estimate of the number of untransformed cells, attenuated downregulation of target genes, as well as an underestimated multiplet frequency. These findings are mirrored in publicly available datasets, where we find that up to 35% of cells are transduced with a mutant gRNA. Applying GiRAFR hence stands to improve the annotation and quality of single cell CRISPR screens.
METTL14-upregulated miR-6858 triggers cell apoptosis in keratinocytes of oral lichen planus through decreasing GSDMC
Authors: Wang Xiangyu , Li Shuangting , Song Huimin , Ding Yan , Gao Ruifang , Shi Xiaotong , Li Ran , Ge Xuejun 
Published: September 2023

AbstractOral lichen planus (OLP), a chronic inflammatory disorder, is characterized by the massive cell apoptosis in the keratinocytes of oral mucosa. However, the mechanism responsible for triggering oral keratinocyte apoptosis is not fully explained. Here, we identify that Gasdermin C (GSDMC) downregulation contributes to apoptosis in human oral keratinocytes. Mechanistically, we describe that activated nuclear factor kappa B (NF-κB) pathway induces overexpression of methyltransferase-like 14 (METTL14), which increases N6-adenosine methylation (m6A) levels in the epithelial layer of OLP. m6A modification is capable of regulating primary miR-6858 processing and alternative splicing, leading to miR-6858 increases. miR-6858 can bind and promote GSDMC mRNA degradation. Forced expression of GSDMC is able to rescue cell apoptosis in human oral keratinocyte models resembling OLP. Collectively, our data unveil that m6A modification regulates miR-6858 production to decrease GSDMC expression and to trigger keratinocyte apoptosis in the context of OLP.
Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice
Authors: Gallardo-Caballero M. , Rodríguez-Moreno C. B. , Álvarez-Méndez L. , Terreros-Roncal J. , Flor-García M. , Moreno-Jiménez E. P. , Rábano A. , Llorens-Martín M. 
Published: September 2023

AbstractAdult hippocampal neurogenesis (AHN) gives rise to new neurons throughout life. This phenomenon takes place in more than 120 mammalian species, including humans, yet its occurrence in the latter was questioned after one study proposed the putative absence of neurogenesis markers in the adult human hippocampus. In this regard, we showed that prolonged fixation impedes the visualization of Doublecortin+ immature neurons in this structure, whereas other authors have suggested that a dilated post-mortem delay (PMD) underlies these discrepancies. Nevertheless, the individual and/or additive contribution of fixation and the PMD to the detection (or lack thereof) of other AHN markers has not been studied to date. To address this pivotal question, we used a tightly controlled experimental design in mice, which allowed the dissection of the relative contribution of the aforementioned factors to the visualization of markers of individual AHN stages. Fixation time emerged as the most prominent factor globally impeding the study of this process in mice. Moreover, the visualization of other particularly sensitive epitopes was further prevented by prolonged PMD. These results are crucial to disambiguate current controversies related to the occurrence of AHN not only in humans but also in other mammalian species.