bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme
Authors: Gao Wenhui , He Jiuyang , Chen Lei , Meng Xiangqin , Ma Yana , Cheng Liangliang , Tu Kangsheng , Gao Xingfa , Liu Cui , Zhang Mingzhen , Fan Kelong , Pang Dai-Wen , Yan Xiyun 
Published: January 2023

AbstractNanozymes with superoxide dismutase (SOD)-like activity have attracted increasing interest due to their ability to scavenge superoxide anion, the origin of most reactive oxygen species in vivo. However, SOD nanozymes reported thus far have yet to approach the activity of natural enzymes. Here, we report a carbon dot (C-dot) SOD nanozyme with a catalytic activity of over 10,000 U/mg, comparable to that of natural enzymes. Through selected chemical modifications and theoretical calculations, we show that the SOD-like activity of C-dots relies on the hydroxyl and carboxyl groups for binding superoxide anions and the carbonyl groups conjugated with the π-system for electron transfer. Moreover, C-dot SOD nanozymes exhibit intrinsic targeting ability to oxidation-damaged cells and effectively protect neuron cells in the ischemic stroke male mice model. Together, our study sheds light on the structure-activity relationship of C-dot SOD nanozymes, and demonstrates their potential for treating of oxidation stress related diseases.
The unifying catalytic mechanism of the RING-between-RING E3 ubiquitin ligase family
Authors: Wang Xiangyi S. , Cotton Thomas R. , Trevelyan Sarah J. , Richardson Lachlan W. , Lee Wei Ting , Silke John , Lechtenberg Bernhard C. 
Published: January 2023

AbstractThe RING-between-RING (RBR) E3 ubiquitin ligase family in humans comprises 14 members and is defined by a two-step catalytic mechanism in which ubiquitin is first transferred from an E2 ubiquitin-conjugating enzyme to the RBR active site and then to the substrate. To define the core features of this catalytic mechanism, we here structurally and biochemically characterise the two RBRs HOIL-1 and RNF216. Crystal structures of both enzymes in their RBR/E2-Ub/Ub transthiolation complexes capturing the first catalytic step, together with complementary functional experiments, reveal the defining features of the RBR catalytic mechanism. RBRs catalyse ubiquitination via a conserved transthiolation complex structure that enables efficient E2-to-RBR ubiquitin transfer. Our data also highlight a conserved RBR allosteric activation mechanism by distinct ubiquitin linkages that suggests RBRs employ a feed-forward mechanism. We finally identify that the HOIL-1 RING2 domain contains an unusual Zn2/Cys6 binuclear cluster that is required for catalytic activity and substrate ubiquitination.
Sub-nanometer-scale mapping of crystal orientation and depth-dependent structure of dislocation cores in SrTiO3
Authors: Sha Haozhi , Ma Yunpeng , Cao Guoping , Cui Jizhe , Yang Wenfeng , Li Qian , Yu Rong 
Published: January 2023

AbstractDefects in crystals play a fundamental role in modulating mechanical, electrical, luminescent, and magnetic behaviors of materials. However, accurate measurement of defect structures is hindered by symmetry breaking and the corresponding complex modifications in atomic configuration and/or crystal tilt at the defects. Here, we report the deep-sub-angstrom resolution imaging of dislocation cores via multislice electron ptychography with adaptive propagator, which allows sub-nanometer scale mapping of crystal tilt in the vicinity of dislocation cores and simultaneous recovery of depth-dependent atomic structure of dislocations. The realization of deep-sub-angstrom resolution and depth-dependent imaging of defects shows great potential in revealing microstructures and properties of real materials and devices.
Skin-integrated stretchable actuators toward skin-compatible haptic feedback and closed-loop human-machine interactions
Authors: Chen Si , Chen Yanjun , Yang Ji , Han Teng , Yao Shanshan 
Published: January 2023

AbstractSkin-integrated haptic interfaces that can relay a wealth of information from the machine to the human are of great interest. However, existing haptic devices are not yet able to produce haptic cues that are compatible with the skin. In this work, we present the stretchable soft actuators for haptic feedback, which can match the perception range, spatial resolution, and stretchability of the skin. Pressure-amplification structures are fabricated using a scalable self-assembly process to ensure an output pressure beyond the skin perception threshold. Due to the minimized device size, the actuator array can be fabricated with a sufficiently high spatial resolution, which makes the haptic device applicable for skin locations with the highest spatial acuity. A haptic feedback system is demonstrated by employing the developed soft actuators and highly sensitive pressure sensors. Two proof-of-concept applications are developed to illustrate the capability of transferring information related to surface textures and object shapes acquired at the robot side to the user side.
Numerical simulation of carbon steel atmospheric corrosion under varying electrolyte-film thickness and corrosion product porosity
Authors: Li Wenchao , Liu Kangning , Wu Jiangshun , Lian Qinglin , Qiang Yujie , Pan Jinshan , Jin Ying 
Published: January 2023

AbstractA finite element model is developed to study dynamics of atmospheric corrosion of carbon steel, focusing on the influence of thin electrolyte film thickness under varying corrosion product porosity. Calculations have been done to evaluate the impact of electrolyte film thickness and corrosion product porosity on oxygen diffusion path, and the hindrance effect of corrosion products on the metal surface activity. The time evolution of corrosion current density and controlling steps in the corrosion process are explored. When the corrosion products are loose, oxygen diffusion is the dominant controlling step, and the thicker the electrolyte film, the lower the corrosion rate. When they are dense, the corrosion process is controlled by the mixture of oxygen diffusion and the surface discharge. The oxygen diffusion path is determined only by the corrosion product porosity, and therefore the corrosion rate is not affected by the electrolyte film thickness.
Experimental investigation of porous carbon for cooling and desalination applications
Authors: Alsaman Ahmed S. , Ahmed M. Salem , Ibrahim E. M. M. , Ali Ehab S. , Farid A. M. , Askalany Ahmed A. 
Published: January 2023

AbstractAdsorption cooling and desalination (ACD) system presents a solution for water, energy, and environmental dilemma. This study expresses an enhancement of adsorption system performance in terms of cooling and desalination effects by improving an activated carbon Maxsorb III (Max) as an adsorbent. Max has been initially treated with HCl. Then, it has been activated by impregnating it in salt hydrates ((NH4)2CO3). Characterization methods, including XRD, N2 and water adsorption isotherm, and water adsorption kinetics have been conducted for raw Max, treated Max, and activated Max with (NH4)2CO3. The experimental values have been fitted with the Dubinin & Astakhov equilibrium model for isotherm and the model of linear driving force for kinetics. These fitted parameters have been utilized in a previously validated model to estimate adsorption cooling-desalination system performance with and without heat recovery. Max/(NH4)2CO3 achieves a water uptake of 0.53 kgH2O.kg-1. Max/(NH4)2CO3 produces 13.2 m3.ton-1 of freshwater per day with a specific cooling power of 373 W.kg-1 and 0.63 COP. Also, the freshwater reaches 22.5 m3.ton-1 of Max/(NH4)2CO3 per day with condenser-evaporator heat recovery. These results indicate the potential of utilizing the Max material in dual cooling and desalination applications to achieve double what silica gel can offer.
Periclase deforms more slowly than bridgmanite under mantle conditions
Authors: Cordier Patrick , Gouriet Karine , Weidner Timmo , Van Orman James , Castelnau Olivier , Jackson Jennifer M. , Carrez Philippe 
Published: January 2023

AbstractTransport of heat from the interior of the Earth drives convection in the mantle, which involves the deformation of solid rocks over billions of years. The lower mantle of the Earth is mostly composed of iron-bearing bridgmanite MgSiO3 and approximately 25% volume periclase MgO (also with some iron). It is commonly accepted that ferropericlase is weaker than bridgmanite1. Considerable progress has been made in recent years to study assemblages representative of the lower mantle under the relevant pressure and temperature conditions2,3. However, the natural strain rates are 8 to 10 orders of magnitude lower than in the laboratory, and are still inaccessible to us. Once the deformation mechanisms of rocks and their constituent minerals have been identified, it is possible to overcome this limitation thanks to multiscale numerical modelling, and to determine rheological properties for inaccessible strain rates. In this work we use 2.5-dimensional dislocation dynamics to model the low-stress creep of MgO periclase at lower mantle pressures and temperatures. We show that periclase deforms very slowly under these conditions, in particular, much more slowly than bridgmanite deforming by pure climb creep. This is due to slow diffusion of oxygen in periclase under pressure. In the assemblage, this secondary phase hardly participates in the deformation, so that the rheology of the lower mantle is very well described by that of bridgmanite. Our results show that drastic changes in deformation mechanisms can occur as a function of the strain rate.
NeuroLINCS Proteomics: Defining human-derived iPSC proteomes and protein signatures of pluripotency
Authors: Matlock Andrea D. , Vaibhav Vineet , Holewinski Ronald , Venkatraman Vidya , Dardov Victoria , Manalo Danica-Mae , Shelley Brandon , Ornelas Loren , Banuelos Maria , Mandefro Berhan , Escalante-Chong Renan , Li Jonathan , Finkbeiner Steve , Fraenkel Ernest , Rothstein Jeffrey , Thompson Leslie , Sareen Dhruv , Svendsen Clive N. ,   NIH NeuroLINCS Consortium, Van Eyk Jennifer E. , Ho Ritchie , Wassie Brook , Patel-Murray Natasha , Milani Pamela , Adam Miriam , Sachs Karen , Lenail Alex , Ramamoorthy Divya , Daigle Gavin , Hussain Uzma , Kaye Julia , Lima Leandro , Kalra Jaslin , Coyne Alyssa , Lim Ryan G , Wu Jie , Stocksdale Jennifer , Thompson Terri G , Van Eyk Jennifer E. 
Published: January 2023

AbstractThe National Institute of Health (NIH) Library of integrated network-based cellular signatures (LINCS) program is premised on the generation of a publicly available data resource of cell-based biochemical responses or “signatures” to genetic or environmental perturbations. NeuroLINCS uses human inducible pluripotent stem cells (hiPSCs), derived from patients and healthy controls, and differentiated into motor neuron cell cultures. This multi-laboratory effort strives to establish i) robust multi-omic workflows for hiPSC and differentiated neuronal cultures, ii) public annotated data sets and iii) relevant and targetable biological pathways of spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS). Here, we focus on the proteomics and the quality of the developed workflow of hiPSC lines from 6 individuals, though epigenomics and transcriptomics data are also publicly available. Known and commonly used markers representing 73 proteins were reproducibly quantified with consistent expression levels across all hiPSC lines. Data quality assessments, data levels and metadata of all 6 genetically diverse human iPSCs analysed by DIA-MS are parsable and available as a high-quality resource to the public.
A literature-derived dataset on risk factors for dry eye disease
Authors: Yang Wanju , Yang Kuiliang , Pan Yumiao , Wu Shangcao , Chen Xiangxi , Shen Lei , Zeng Qingyan , Wu Jianhua , Lv Minzhi , Zhang Junyan , Yang Yanning , Xing Yiqiao 
Published: January 2023

AbstractDry eye disease (DED) is a common disease associated with disorder of tear secretion. Research on risk factors for DED, such as depression, arthritis, thyroid disease, stroke and diabetes, is important to facilitate its diagnosis and prognosis. We created a dataset on risk factors for DED (DrDED) with public access that can provide up-to-date and validated data acquired from systematically searched and screened, high-quality studies. The established DrDED contained 119 studies published between 2000 and 2022. The range of the study sample size was from 43 to 4,871,504. The study types were, as follows: cross-sectional (n = 92), retrospective cohort (n = 9), prospective cohort (n = 10), and case-control (n = 8) studies. Data from eligible studies were collected and presented for the present study, including the publication information, study characteristics, definition and prevalence of the disease, and risk factors for DED, together with the strength of association. With the publication of new relevant studies, the DrDED will be updated, and the data will be made accessible to the users.

Design Type(s)
Dataset creation objective

Measurement Type(s)
Patient outcome • scientific publication • risk factors • dry eye disease

Technology Type(s)
Digital curation • documenting • meta-analysis

Factor Type(s)
Depression • arthritis • thyroid disease • stroke disease • diabetes

Sample Characteristic(s)
Homo sapiens • dry eye disease • global
High-performance Fe(Se,Te) films on chemical CeO2-based buffer layers
Authors: Piperno L. , Vannozzi A. , Augieri A. , Masi A. , Mancini A. , Rufoloni A. , Celentano G. , Braccini V. , Cialone M. , Iebole M. , Manca N. , Martinelli A. , Meinero M. , Putti M. , Meledin A. 
Published: January 2023

AbstractThe fabrication of a Fe-based coated conductor (CC) becomes possible when Fe(Se,Te) is grown as an epitaxial film on a metallic oriented substrate. Thanks to the material’s low structural anisotropy, less strict requirements on the template microstructure allow for the design of a simplified CC architecture with respect to the REBCO multi-layered layout. This design, though, still requires a buffer layer to promote the oriented growth of the superconducting film and avoid diffusion from the metallic template. In this work, Fe(Se,Te) films are grown on chemically-deposited, CeO2-based buffer layers via pulsed laser deposition, and excellent properties are obtained when a Fe(Se,Te) seed layer is used. Among all the employed characterization techniques, transmission electron microscopy proved essential to determine the actual effect of the seed layer on the final film properties. Also, systematic investigation of the full current transport properties J(θ, H, T) is carried out: Fe(Se,Te) samples are obtained with sharp superconducting transitions around 16 K and critical current densities exceeding 1 MA cm−2 at 4.2 K in self-field. The in-field and angular behavior of the sample are in line with data from the literature. These results are the demonstration of the feasibility of a Fe-based CC, with all the relative advantages concerning process simplification and cost reduction.
Candida albicans-induced activation of the TGF-β/Smad pathway and upregulation of IL-6 may contribute to intrauterine adhesion
Authors: Zhao Xingping , Sun Dan , Zhang Aiqian , Huang Huan , Li Yueran , Xu Dabao 
Published: January 2023

AbstractIatrogenic injury to endometrial tissue is the main cause of intrauterine adhesions (IUA) and infection can also damage the endometrium. The microbiota plays an important role in the health of the female reproductive tract. However, the mechanism is still unclear. In total, 908 patients with IUA and 11,389 healthy individuals were retrospectively selected for this clinical study. Participant information including vaginal microecological results and human papillomavirus (HPV) status were collected. Univariate and multivariate logistic regression analyses were used to identify the factors related to IUA. Next, animal experiments were performed in a curettage-induced IUA rat model. After the procedure, rats in the experimental group received a vaginal infusion of a Candida albicans (C. albicans) fungal solution. On days 3, 7, and 14 after curettage and infusion, the expression levels of IL-6, fibrotic pathway-related factors (TGF-β1, Smad 2, and COL1), and estrogen receptor (ER) and progesterone receptor (PR) in rat endometrial tissues were assessed. Fungal infection of the reproductive tract was found to be an independent risk factor for IUA (P < 0.05). The inflammatory response and degree of fibrosis were greater in rats infected with C. albicans than in the controls. The levels of IL-6, TGF-β1, Smad 2, and COL1 expression in endometrial tissues were significantly higher in the experimental group than in the control group (P < 0.05). However, the ER and PR levels were lower in the IUA group than in the non-IUA group (P < 0.05). C. albicans infection may be related to IUA. C. albicans elicits a strong inflammatory response that can lead to more severe endometrial fibrosis.
Retarding breast tumor growth with nanoparticle-facilitated intravenous delivery of BRCA1 and BRCA2 tumor suppressor genes
Authors: Ibnat Nabilah , Chowdhury Ezharul Hoque 
Published: January 2023

AbstractGene augmentation therapy entails replacement of the abnormal tumor suppressor genes in cancer cells. In this study, we performed gene augmentation for BRCA1/2 tumor suppressors in order to retard tumor development in breast cancer mouse model. We formulated inorganic carbonate apatite (CA) nanoparticles (NPs) to carry and deliver the purified BRCA1/2 gene- bearing plasmid DNA both in vitro and in vivo. The outcome of BRCA1/2 plasmid-loaded NPs delivery on cellular viability of three breast cancer cell lines such as MCF-7, MDA-MB-231 and 4T1 were evaluated by MTT assay. The result in MCF-7 cell line exhibited that transfection of BRCA 1/2 plasmids with CA NPs significantly reduced cancer cell growth in comparison to control group. Moreover, we noticed a likely pattern of cellular cytotoxicity in 4T1 murine cancer cell line. Following transfection with BRCA1 plasmid-loaded NPs, and Western blot analysis, a notable reduction in the phospho-MAPK protein of MAPK signaling pathway was detected, revealing reduced growth signal. Furthermore, in vivo study in 4T1 induced breast cancer mouse model showed that the tumor growth rate and final volume were decreased significantly in the mouse group treated intravenously with BRCA1 + NPs and BRCA2 + NPs formulations. Our results established that BRCA1/2 plasmids incorporated into CA NPs mitigated breast tumor growth, signifying their application in the therapy for breast cancer.
Effects of monensin supplementation on lactation performance of dairy cows: a systematic review and dose–response meta‑analysis
Authors: Rezaei Ahvanooei M. R. , Norouzian M. A. , Piray A. H. , Vahmani P. , Ghaffari M. H. 
Published: January 2023

AbstractThe aim of this study was to conduct a comprehensive review with meta-analysis to determine the effects of the dose–response relationship between monensin supplementation and dairy cow performance and milk composition. Results from 566 full-text articles and 48 articles with 52 studies were meta-analyzed for pooled estimates. Monensin supplementation up to 23 ppm increased milk production, with the optimal dose being 12.6 ppm. Monensin supplementation at doses ranging from 16 to 96 ppm increased milk production in the prepartum phase (− 28 to 0 day relative to calving). From 60 to 150 DIM, monensin supplementation up to 21 ppm had a significant positive effect on this outcome, while supplementation in the 37 to 96 ppm range caused a decrease in this variable. At 0 to 60 and > 150 DIM, monensin supplementation had no effect on milk yield. At dosages of 22 to 96 ppm, 12 to 36 ppm, and below 58 ppm and 35 ppm, respectively, monensin supplementation resulted in significant decreases in dry matter intake (DMI), milk protein percentage, milk fat percentage, and milk fat yield. Overall, based on the results of this meta-analysis and considering all variables, the recommended optimal dose of monensin could be about 16 ppm.
Generation of highly realistic microstructural images of alloys from limited data with a style-based generative adversarial network
Authors: Lambard Guillaume , Yamazaki Kazuhiko , Demura Masahiko 
Published: January 2023

AbstractIn materials science, the amount of observational data is often limited by operating protocols that require a high level of expertise, often machine-dependent, developed for a time-consuming integration of valuable data. Scanning electron microscopy (SEM) is one of those methodologies of characterisation for which the number of observations of a given material is limited to just a few images. In the present study, we present the possibility to artificially inflate the size of SEM image datasets from a limited ($$\mathrm \sim 100s-1000s$$

∼
100
s
-
1000
s

of images) to a virtually unbounded number thanks to a generative adversarial network (GAN). For this purpose, we use one of the latest developments in GAN architectures and training methodologies, the StyleGAN2 with adaptive discriminator augmentation (ADA), to generate a diversity of high-quality SEM images of $$\mathrm 512\times 512$$

512
×
512

pixels. Overall, coarse and fine microstructural details are successfully reproduced when training a StyleGAN2 with ADA from scratch on at most 3000 SEM images, and interpolations between microstructures are performed without significant modifications to the training protocol when applied to natural images.
Students stress patterns in a Kenyan socio-cultural and economic context: toward a public health intervention
Authors: Mutiso Victoria N. , Ndetei David M. , Muia Esther N. , Musyimi Christine , Masake Monicah , Osborn Tom L. , Sourander Andre , Weisz John R. , Mamah Daniel 
Published: January 2023

AbstractThis study aimed at determining the prevalence of stress, different types of stress, their severity and their determinants in Kenyan university, college and high school students. The following tools were administered to 9741 students: (1) Researcher-designed socio-demographic tool, (2) Psychiatric Diagnostic Screening Questionnaire (PDSQ) for psychiatric disorders, (3) WERC Stress Screen for stress, (4) Washington Early Recognition Center Affectivity and Psychosis (WERCAP) screen for psychosis and affectivity, (5) Wealth Index Questionnaire for economic indicators. Descriptive analysis for the prevalence of different types of stress and inferential analysis for stress and independent variables were done. Significant variables (p < 0.05) were fitted into generalized linear model to determine independent predictors. The mean age of the respondents was 21.4 years (range 16–43). Money issues were the commonest stressors while alcohol and drug use were the least. The independent predictors of stress were females, college students and use of gas stove. In conclusion, up to 30% of the students suffer from mild to severe stress. The students experience a wide range of stressors. The most important stressors include money and finances, family related problems and concerns about their future. Our findings suggest a public health approach to create stress awareness in students.