bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial biogenesis as an underlying mechanism involved in the cardioprotective effects of Gallic acid against D‐galactose-induced aging
Authors: Zarei Mohammad , Sarihi Abdolrahman , Zamani Alireza , Raoufi Safoura , Karimi Seyed Asaad , Ramezani-Aliakbari Fatemeh 
Published: January 2023

AbstractAged heart is defined via structural and mitochondrial dysfunction of the heart. However, there is still no impressive compound to suppress and improve the abnormal alterations in cardiac function result from aging. Gallic acid (GA) is known to be an effective agent in improving cardiovascular disorders. In the present study, we exhibit the protective effects of GA against cardiac aging. Male Wistar rats were randomly divvied into four groups: Control, Control treated with GA at 25 mg/kg (GA25), aged rats induced by D-galactose (D-GAL), aged rats treated with GA at 25 mg/kg (D-GAL + GA25).Aging induced by D-GAL at 150 mL/kg via intraperitoneal injection for eight weeks. Aged rats treated with GA at 25 mg/kg (D-GAL GA25) by gavage for eight weeks. The blood samples were used to assessment biochemical factors and heart tissue was assessed for evaluating oxidative stress and the gene expression of molecular parameters. Histological examination of the heart was occurred. The D-GAL rats indicated cardiac hypertrophy, which was associated with reduced antioxidant activity of enzyme, increased oxidative marker and alterations in Sirtuin 1 (SIRT1), Peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha and Transcription Factor A, Mitochondrial (TFAM) genes expression in comparison to the control animals. Co-treatment with GA improved all these alterations. Taken together, GA could protect the heart against D-GAL-induced aging via antioxidant effects, and the enhancement of SIRT1, PGC-1α, and TFAM genes expression.
Outcomes of early endoscopic realignment for pelvic fracture urethral injury
Authors: Chaker Kays , Ouanes Yassine , Chedly Wassim Ben , Trigui Mohamed , Mosbahi Boutheina , Chbeb Oumayma , Elabed Wiem , Bibi Mokhtar , Rahoui Moez , Dali Kheireddine Mrad , Ammous Adel , Nouira Yassine 
Published: January 2023

Abstract
Introduction:
Post-traumatic rupture of the posterior urethra is a serious injury that can compromise the micturition and erectile prognosis of the often-young patient. The management of this lesion is still controversial, leaving the choice between early endoscopic realignment or suprapubic catheterization with deferred urethroplasty. The objective of this study was to report our clinical experience and outcomes with early endoscopic realignment (EER) for patients with pelvic fracture urethral injury.
Patients and Methods:
We underwent a retrospective review of patients with pelvic fracture associated urethral injury who underwent EER from 2010 to 2020. Preoperative, perioperative, and postoperative outcome data were collected. Complications for the surgical procedure was analyzed, as well as post-operative stenosis, urinary incontinence and erectile dysfunction. The primary endpoint was success, defined as satisfying micturition with no urethral stricture at the time of last follow up.
Results:
A total of 26 patients underwent primary endoscopic realignment. The median duration from injury to EER was 15.4 ± 10.25 hours. No patient experienced complications from endoscopic realignment. EER was successful in 16 patients (61.53%) at a median follow up of 34 months (18–54). Ten patients (38.46%) developed a urethral stricture during follow up. Seven patients (26.92%) were treated by one or two direct visual internal urethrotomy. Only 3 patients (11.53%) required urethroplasty. There were no urethroplasty failure after previous EER. Two patients (7.69%) reported stress urinary incontinence after EER. Four patients (15.88%) developed de novo erectile dysfunction.
Conclusion:
Early endoscopic realignment allows some patients to avoid a heavier surgical treatment, and doesn’t compromise the realization of a later urethroplasty.
Penaeus vannamei nursery in a synbiotic system: Effects of different forms of artificially salinized in low-salinity water
Authors: Limeira Agatha Catharina , Silva Gênison Carneiro , Costa Gisely Karla Almeida , Silva Suzianny Maria Bezerra Cabral , Galvez Alfredo Olivera , Brito Luis Otavio 
Published: January 2023

Abstract
This study aimed to evaluate the effect of different forms of artificially salinized on the zootechnical performance, TCBS and Sabouraud Dextrose counts in Penaeus vannamei juveniles reared in a synbiotic system. Four treatments were established in triplicate using a completely randomized design: SD (diluted seawater); LCSM (low-cost salt mix with freshwater); CS (commercial salt); and SW (seawater) in experimental tanks of 60 L, with a density of 2,000 PL m− 3 for 40 days. An ammonia stress test was performed at the end of the culture and TCBS and Sabouraud Dextrose counts in gut was performed at the 20th and 40th. The synbiotic system proved to be effective in the control of nitrogen compounds. The values of calcium, magnesium and total hardness remained above 30, 75 and 400 mg L− 1, respectively in low salinity treatments. Better values of final average weight were obtained with the different forms of artificially salinized (LCSM, SD and CS), but survival was lower when compared to SW. There was no influence of different forms of artificially salinized on bacterial counts of TCBS and Sabouraud Dextrose, and on resistance to ammonia stress. Based on the results obtained, it can be concluded that it is possible to rear P. vannamei juveniles in artificially salinized water of low salinity (3 g L− 1) in a synbiotic system.
Comparing variants related to chronic diseases from genome- wide association study (GWAS) and the cancer genome atlas (TCGA)
Authors: Jeon Soohyun , Park Chaewon , Kim Jineui , Lee Jung Hoon , Joe Sung-yune , Ko Young Kyung , Gim Jeong-An 
Published: January 2023

Abstract
Background
Several genome-wide association studies (GWAS) have been performed to identify variants related to chronic diseases. Somatic variants in cancer tissues are associated with cancer development and prognosis. Expression quantitative trait loci (eQTL) and methylation QTL (mQTL) analyses were performed on chronic disease-related variants in TCGA dataset.
Methods
MuTect2 calling variants for 33 carcinomas from TCGA and 296 GWAS variants provided by LocusZoom were used. At least one mutation was found in TCGA 22 carcinomas and LocusZoom 23 studies. Differentially expressed genes (DEGs) and differentially methylated regions (DMRs) from the three carcinomas (TCGA-COAD, TCGA-STAD, and TCGA-UCEC). Variants were mapped to the world map using population locations of the 1000 Genomes Project (1GP) populations. Decision tree analysis was performed on the discovered features and survival analysis was performed according to the cluster.
Results
Based on the DEGs and DMRs with clinical data, the decision tree model classified seven and three nodes in TCGA-COAD and TCGA-STAD, respectively. A total of 11 variants were commonly detected from TCGA and LocusZoom, and eight variants were selected from the 1GP variants, and the distribution patterns were visualized on the world map.
Conclusions
Variants related to tumors and chronic diseases were selected, and their geological regional 1GP-based proportions are presented. The variant distribution patterns could provide clues for regional clinical trial designs and personalized medicine.
Spatiotemporal expression of Rest in the brain of Ts1Cje mouse model of Down syndrome
Authors: Lim Chong-Teik , Lam Xin-Jieh , Crystal Arthini-Arrumugam , Huang Tan , Jusoh Norhazlin , Cheah Pike-See , Ling King-Hwa 
Published: January 2023

Abstract
Introduction: Down syndrome (DS) is a common genetic disorder caused by full or partial trisomy of human chromosome 21. DS individuals usually have poor neurological development with neuropsychiatric manifestations. Repressor element-1 silencing transcription factor (REST) is the key regulator for epigenetic neuronal gene expression. REST nuclear translocation is crucial to exert repression on target genes transcriptionally. A comprehensive spatiotemporal profiling of Rest expression was performed on the Ts1Cje mouse brain to reveal its association with DS neuropathology development.
Methods: Over-representation analysis of Ts1Cje differentially expressed genes (DEGs) with mouse REST targets was performed. The cerebral cortex, hippocampus and cerebellum of Ts1Cje and wildtype (WT) mice were procured at postnatal - P1, P15, P30, and P84 and embryonic - E14 and P1.5 development timepoints[User1] . RNAs from the brain tissues and cultured neurospheres were analysed with qPCR to determine the spatiotemporal profile of Rest expression. Western blot and immunohistochemistry (IHC) staining were performed to determine the level of REST expression and nuclear localisation.
Results: Over-representation analysis showed the Ts1Cje DEGs are significantly overlapped with mouse REST target genes. QPCR and Western blot analysis revealed a significant downregulation of Rest in neurospheres and protein expression in Ts1Cje compared to WT. Furthermore, IHC staining showed a consistent perinuclear marginalisation of REST, indicating impaired nuclear translocation in the Ts1Cje brain.
Conclusion:DEGs in the Ts1Cje tissues are potentially caused by the loss of REST functions. Dysregulated Rest expression at the early neurodevelopmental stage may cause premature neurodifferentiation, neural stem cell pool depletion, and disrupt early cell fate determination. The loss of nuclear REST function may cause neuroprotection and stress resilience deficits.
Efficiency of cultivation of sugar sorghum in the conditions of rice systems of the Kyzylorda region
Authors: Olzhabayeva Axaule Ondasynovna , Baimanov Zhanuzak Nurdildaevich , Bilalovich Begaliyev Kanagat , Urunbasarovna Bulanbayeva Perizat , Temirkhankyzy Aldambergenova Gulzinat 
Published: January 2023

Abstract
In the economy of Kazakhstan, agriculture occupies one of the leading places and is a vital sector of the national economy. In the Kyzylorda region, a decrease in land productivity (about 21.1 thousand hectares or 9.4% of the total used area (22.4 thousand hectares) was due to secondary salinization of about 62.8 thousand hectares, waterlogging of 22.3 thousand hectares or 29.6% of all irrigated lands in the region (287,525 hectares), loss of land resources and transformation into unproductive salt deserts. All this is a consequence of mismanagement in relation to the breadwinner of the land.
Of great importance for the sustainable development of crop production in the region is the introduction of non-traditional, salt-tolerant grain fodder and fodder crops that can form a high yield in the conditions of the Kyzylorda region. Within the framework of the FAO project "Integrated natural resource management in drought-prone and saline areas of agricultural production in Central Asia and Turkey", field studies were carried out on the cultivation of sugar sorghum in the saline fields of rice systems of Dostyk Zher LLP, NK Kurylys LLP and PF " Talapker”, which ensured a high yield of rice and efficient use of irrigated land.
Serum anti-PCK1 antibody levels are prognostic factor for the patients with diabetes mellitus
Authors: Namiki Toshiki , Takemoto Minoru , Hayashi Aiko , Yamagata Hiroki , Ishikawa Takahiro , Yokote Koutaro , Li Shu-Yang , Kubota Masaaki , Zhang Bo-Shi , Yoshida Yoichi , Matsutani Tomoo , Mine Seiichiro , Machida Toshio , Kobayashi Yoshio , Terada Jiro , Naito Akira , Tatsumi Koichiro , Takizawa Hirotaka , Nakamura Rika , Kuroda Hideyuki , Iwadate Yasuo , Hiwasa Takaki 
Published: January 2023

Abstract
Background: Autoantibodies develop in autoimmune diseases, cancer, diabetes mellitus (DM),and atherosclerosis-related diseases. However, autoantibody biomarkers have not been successfully examined for diagnosis and therapy.
Methods: Serological identification of antigens through recombinant cDNA expression cloning (SEREX) was used for primary screening of antigens. The cDNA product was expressed in bacteria and purified. Amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) was used to evaluate antibody levels in serum samples.
Results: Phosphoenolpyruvate carboxykinase 1 (PCK1) was recognized as an antigen by serum IgG antibodies in the sera of patients with atherosclerosis. AlphaLISA showed significantly higher serum antibody levels against recombinant PCK1 protein in patients with DM and cardiovascular diseasebut not in those with acute ischemic stroke, transient ischemic attack, or obstructive sleep apnea syndrome, than in healthy donors. The area under the receiver operating characteristic curve for anti-PCK1 antibodies was 0.7024 for DM. The serum anti-PCK1 antibody levels were associated with age, platelet count, and blood pressure. Anti-PCK1-antibody-positive patients showed significantly lower overall survival than the negative patients.
Conclusions: Serum anti-PCK1 antibody levels were strongly associated with DM and weakly but significantly associated with cardiovascular disease. The anti-PCK1 antibody marker is useful for predicting the overall survival of patients with DM.
Knockdown of Rab7B, but not of Rab7A, which antagonistically regulates oligodendroglial cell morphological differentiation, recovers tunicamycin-induced defective differentiation in FBD-102b cells
Authors: Fukushima Nana , Shirai Remina , Sato Takanari , Nakamura Sayumi , Ochiai Arisa , Miyamoto Yuki , Yamauchi Junji 
Published: January 2023

Abstract
In the central nervous system (CNS), insulative myelin sheaths are generated from the differentiated plasma membranes of oligodendrocytes (oligodendroglial cells) and surround neuronal axons to achieve saltatory conduction. Despite the functional involvement of myelin sheaths in the CNS, the molecular mechanism by which oligodendroglial cells themselves undergo differentiation of plasma membranes remains unclear. It also remains to be explored whether their signaling mechanisms can be applied to treating diseases of the oligodendroglial cells. Here we describe that Rab7B of Rab7 subfamily small GTPases negatively regulates oligodendroglial cell morphological differentiation using FBD-102b cells, which are model cells undergoing differentiation of oligodendroglial precursors. Knockdown of Rab7B or Rab7A by the respective specific siRNAs in cells positively or negatively regulated morphological differentiation, respectively. Consistently, these changes were supported by changes on differentiation- and myelination-related structural protein and protein kinase markers. We also found that knockdown of Rab7B has the ability to recover inhibition of morphological differentiation following tunicamycin-induced endoplasmic reticulum (ER) stress, which mimics one of the major molecular pathological causes of hereditary hypomyelinating disorders in oligodendroglial cells, such as Pelizaeus-Merzbacher disease (PMD). These results suggest that the respective molecules among very close Rab7 homologues exhibit differential roles in morphological differentiation and that knocking down Rab7B can recover defective differentiating phenotypes under ER stress, thereby adding Rab7B to the list of molecular therapeutic cues taking advantage of signaling mechanisms for oligodendroglial diseases like PMD.
Long time behavior of a 2D Ginzburg-Landau model with fixed total magnetic flux
Authors: Fan Jishan , Ozawa Tohru 
Published: January 2023

Abstract
We prove the long time behavior of a 2D Ginzburg-Landau system in supercon-ductivity with Coulomb gauge and fixed total magnetic flux.
Mathematics Subject Classifications (2020): 35A05, 35A40, 35K55, 82D55.
A Systematic Pan-Cancer Analysis of SPATS2L, a Potential New Immunological and Prognostic Biomarker
Authors: Cao Yan , Yang Yuwei , Li Qiao , Wang Xinjing , Lu Hengzhi , Guo Zongwei , Yao Chen , Xiao Li , Xie Lixin 
Published: January 2023

Abstract
SPATS2L (Spermatogenesis-associated serine-rich 2-like) is anintranucleolar stress-response protein involved in chromosomal organization, ribosomal biogenesis, and translational control. Although there is mounting evidence that SPATS2L was involved in the carcinogenesis of some cancers, no thorough pan-cancer investigation of SPATS2L is available so far. Based on multiple databases, including TCGA, GTEx, CCLE, cBioPortal, TIMER2, ImmuCellAI, GDSC, and Reactome, we analyzed the expression, prognosis, DNA methylation, tumor mutational burden (TMB), microsatellite instability (MSI), immune cell infiltration, drug sensitivity, and clinicopathological and prognostic relevance of SPATS2L in pan-cancer including 33 types of cancers. SPATS2L expression was dramatically increased in a variety of malignancies, while it was low in ACC, KICH, and LAML as indicated by multiple databases and confirmed by immunohistochemistry assays. Importantly, SPATS2L has been found to have prognostic and clinicopathological importance in several malignancies. SPATS2L expression was also linked to TMB and MSI in 9 types of cancers, and there was a link between SPATS2L expression and DNA methylation in 28 types of cancers. SPATS2L was also found to be highly linked with immune cell infiltration, ICP expression, stromal score, immune score, and ESTIMATE score in various malignancies, demonstrating their regulatory roles on the TME. Consistently, the results of GSEA and GSVA analyses revealed a substantial link between SPATS2L and certain cellular immunological responses. Finally, SPATS2L was found to be strongly linked to 173 anti-tumor drugs. This study indicated that SPATS2L might be a potential cancer biomarker for the prognosis and immunotherapeutic response. SPATS2L expression in cancers may be involved in the regulation of the tumor immune microenvironment and drug sensitivity, which may be a new-targeted molecule for developing anti-tumor drugs and immunotherapy.
Ion-injection bipolar membrane electrodialysis realizes 8+ mol/L caustic soda conversion from the brine stream
Authors: Fu Rong , Wang Huangying , Yan Junying , Li Ruirui , Wang Baoying , Jiang Chenxiao , Wang Yaoming , Xu Tongwen 
Published: January 2023

Abstract
The chlor-alkali process is currently the predominanttechnology for caustic soda production but has several major challenges,such as huge energy demand, and the emission of a large amount of chlorine, which cannot be completely consumed in downstream industries. Bipolar membrane electrodialysis (BMED) is an emerging technology to produce caustic soda with near zero chlorine emission by accelerating water splitting in bipolar membranes. However, the gradualdepletion of feedstock and the unbalanced behavior between water splitting and salt migration have led to the produced acid and base concentrations beingrelatively low for further utilization. Herein, we proposea novel ion-injection bipolar membrane electrodialysis (IJBMED) approach, which achieves a continuous feedstock supply. This IJBMED design is capable of boosting water splitting in abipolar membrane with an ultrahigh current density while avoiding concentration polarization in the anion- and cation-exchange membranes. Therefore, the IJBMED system enables one-step conversion of the NaCl-containing brine stream into 8.4 mol/L NaOH with specific energy consumptions of 870 kWh/t NaOH (24 wt.%→26 wt.%) and 2740 kWh/t NaOH (0→26 wt.%). This bench-scale experiment proved that IJBMED is a promising caustic base production technology that might be aplausible supplement or even compete with the chlor-alkali approach.
Indigenous Herbal Medicine Use and its Associated Factors among Pregnant Women attending antenatal care at Public Health Facilities in Dire Dawa Administration, EasternEthiopia, 2022
Authors: Mohammed Aminu , Amsalu Bezabih , Hailu Mikiale , Manaye Yibekal , Nuri Aliya , Weldeamanuel Tadesse , Belay Yalelet , Abera Legesse , Sintayehu Yitagesu , Sema Alekaw , Aliyi Ahmedin , Legesse Henok , Sertsu Addisu 
Published: January 2023

Abstract
Background: Indigenous herbal medicine use during pregnancy is a common phenomenon worldwide, particularly in low resource countries like Ethiopia, mainly due to their cost, perceived efficiency in treatment, and ease of access. But so far, studies across Ethiopia are variable and inconsistent and in the study area. Therefore, this study was aimed at assessing indigenous herbal medicine use and its associated factors among pregnant women.
Methods: A facility-based cross-sectional study was conducted in public health facilities in the Dire Dawa Administration, eastern Ethiopia, from October 10 to November 10, 2022, among pregnant women selected using a simple random sampling technique. Data were collected through face-to-face interviews using a pre-tested structured questionnaire, and data were entered and cleaned by Epi DATA (Version 3.1) and analyzed using SPSS (Version 22). A P-value < 0.25 at bivariate to select variables for multivariate and ≤ 0.05 at multivariate with 95% confidence intervals was considered statistically significant.
Results: A total of 628 participants were included, yielding a response rate of 95.15 %. During their current pregnancy, 47.8% (95% CI: 43.8–51.6%) pregnant women used indigenous herbal medicine(IHMs). The predictors were education level: no formal education (AOR: 5.47, 95%CI: 2.40-12.46), primary level (AOR: 4.74, 95%CI: 2.15-10.44), rural residence (AOR: 2.54, 95%CI: 1.71-3.77), being a housewife (AOR: 4.15, 95%CI: 1.83-9.37), number of antenatal care visits (AOR: 2.58, 95%CI: 1.27-5.25), and knowledge of IHMs (AOR: 4.58, 95% CI: 3.02-6.97).
Conclusion: The use of indigenous herbal medicine during pregnancy was as common and widespread as other research done in different areas. The predictors were residence, education level, occupation, number of antenatal care visits, and knowledge. The most commonly used herbal medicines were Lepidium sativum, Vernonia amygdalina, Moringa oleifera, Linum usitatissimum, Zingiber officinale, Eucalyptusglobulus, and Trigonella foenum-graecum. The most common indications were related to gastro-intestinal problems: intestinal parasites, nausea and vomiting, constipation, stomach aches, indigestion, and abdominal cramps. It was recommended that, considering their residence and education level, pregnant women during their ANC visits be made aware of the potential benefits and risks of indigenous herbal medicine, and that more research be conducted to confirm their efficacy and safety during pregnancy.
RAI3 expression is not associated with clinical outcomes of patients with non-small cell lung cancer
Authors: Melling Nathaniel , Reeh Matthias , Ghadban Tarik , Tachezy Michael , Hajek André , Izbicki Jakob Robert , Grupp Katharina 
Published: January 2023

Abstract
Purpose
Retinoic acid inducible protein 3 (RAI3) has been suggested as prognostic biomarker in several cancer types. The present study aimed to examine the role of RAI3 expression in non-small cell lung cancers (NSCLCs).
Methods
RAI3 protein expression was evaluated by immunohistochemistry in tissue microarray (TMA) sections from a retrospective cohort of more than 600 surgically resected NSCLCs and results were compared with clinicopathological features and follow-up data.
Results
While membranous RAI3 immunostaining was always strong in benign lung, strong RAI3 staining was only detectable in 14.7% of 530 interpretable NSCLCs. Within NSCLC subtypes, immunostaining intensity for RAI3 was significantly decreased in large cell lung cancers (LCLCs) and squamous cell carcinomas (SQCCs) relative to lung adenocarcinomas (LUACs) (P < 0.0001 each). However, RAI3 staining was neither associated with pathological features of NSCLCs nor with survival of patients (P = 0.6915).
Conclusion
Our study shows that RAI3 expression was not associated with clinical outcomes of NSCLC patients and cannot be considered as prognostic marker in lung cancer patients.