bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiological ramifications of constrained collective cell migration
Authors: Leclech Claire , Barakat Abdul I. 
Published: April 2023

AbstractConstraining collective cell migration in vitro using different types of engineered substrates such as microstructured surfaces or adhesive patterns of different shapes and sizes often leads to the emergence of specific patterns of motion. Recently, analogies between the behavior of cellular assemblies and that of active fluids have enabled significant advances in our understanding of collective cell migration; however, the physiological relevance and potential functional consequences of the resulting migration patterns remain elusive. Here we describe the different patterns of collective cell migration that have been reported in vitro in response to geometrical constraints, explore the in vivo pertinence of the in vitro systems used to impose the geometrical constraints, and discuss the potential physiological ramifications of the collective migration patterns that emerge as a result of physical constraints. We conclude by highlighting key upcoming challenges in the exciting field of constrained collective cell migration.
Solving the conundrum of intra‐specific variation in metabolic rate: A multidisciplinary conceptual and methodological toolkit
Authors: Metcalfe Neil B. , Bellman Jakob , Bize Pierre , Blier Pierre U. , Crespel Amélie , Dawson Neal J. , Dunn Ruth E. , Halsey Lewis G. , Hood Wendy R. , Hopkins Mark , Killen Shaun S. , McLennan Darryl , Nadler Lauren E. , Nati Julie J.H. , Noakes Matthew J. , Norin Tommy , Ozanne Susan E. , Peaker Malcolm , Pettersen Amanda K. , Przybylska‐Piech Anna , Rathery Alann , Récapet Charlotte , Rodríguez Enrique , Salin Karine , Stier Antoine , Thoral Elisa , Westerterp Klaas R. , Westerterp‐Plantenga Margriet S. , Wojciechowski Michał S. , Monaghan Pat 
Published: April 2023

AbstractResearchers from diverse disciplines, including organismal and cellular physiology, sports science, human nutrition, evolution and ecology, have sought to understand the causes and consequences of the surprising variation in metabolic rate found among and within individual animals of the same species. Research in this area has been hampered by differences in approach, terminology and methodology, and the context in which measurements are made. Recent advances provide important opportunities to identify and address the key questions in the field. By bringing together researchers from different areas of biology and biomedicine, we describe and evaluate these developments and the insights they could yield, highlighting the need for more standardisation across disciplines. We conclude with a list of important questions that can now be addressed by developing a common conceptual and methodological toolkit for studies on metabolic variation in animals.
Quality by design‐steered development and validation of analytical and bioanalytical methods for raloxifene: Application of Monte Carlo simulations and variance inflation factor
Authors: Pant Anjali , Sharma Gajanand , Saini Sumant , Jain Atul , Barnwal Ravi Pratap , Singh Gurpal , Singh Bhupinder 
Published: April 2023

AbstractA sensitive, rapid, reproducible, and economical HPLC method is reported for the quantification of raloxifene hydrochloride employing Quality by Design (QbD) principles. Factor screening studies, employing Taguchi design, indicated buffer volume percentage and isocratic flow rate as the critical method parameters (CMPs), which significantly influence the chosen critical analytical attributes, that is, tailing factor and theoretical plate number. Method conditions were subsequently optimized using face‐centered cubic design with magnitude of variance inflation factor for assessing multicollinearity among CMPs. Method operable design region (MODR) was earmarked and liquid chromatographic separation optimized using 0.05 M citrate buffer, acetonitrile, and methanol (57:40:3 v/v/v) as ggmobile phase at 0.9 mL min−1flow rate,λmaxof 280 nm, and column temperature of 40°C. Validation of the developed analytical method was accomplished as per International Council on Harmonization (ICH) guidelines confirming high levels of linearity, precision, accuracy, robustness, and sensitivity. Application of Monte Carlo simulations enabled the attainment of best plausible chromatographic resolution and corroboration of the demarcated MODR. Establishment and validation of the bioanalytical method using rat plasma samples, along with forced degradation and stability studies, corroborated the aptness of developed HPLC methods for drug quantification in the biological fluids, as well as in bulk and marketed dosage forms.
LC–MS/MS method for the quantitation of a dual PI3K/BRD4 inhibitor SF2523 in mouse plasma: Application to plasma protein binding and metabolism studies
Authors: Bala Veenu , Chhonker Yashpal S. , Morales Guillermo A. , Maddeboina Krishnaiah , Pal Dhananjaya , Durden Donald L. , Murry Daryl J. 
Published: April 2023

AbstractA sensitive and selective liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) method was developed and validated for the quantitation of dual PI3K/BRD4 inhibitor SF2523 in mouse plasma. The analysis was performed on a UPLC system connected to a Shimadzu 8060 mass spectrometer by electrospray ionization in positive multiple reaction monitoring mode. Chromatographic separation was carried out on an ACE Excel C18 column with a gradient elution containing 0.1% formic acid and methanol as the mobile phase. The linearity was conducted in the concentration range 0.1–500 ng/ml for SF2523 in 100 μl of plasma. The inter‐ and intra‐batch precision (RSD) were both lower than 13.5%, with the accuracy (percentage bias) ranging from −10.03 to 11.56%. The validated method was successfully applied to plasma protein binding and in vitro metabolism studies. SF2523 was highly bound to mouse plasma proteins (>95% bound). Utilizing mouse S9 fractions, a total of seven phase I and II metabolites were identified with hydroxylation found to be the major metabolic pathway. Metabolite identification included analysis of retention behaviors, molecular weight changes and MS/MS fragment patterns of SF2523 and the metabolites. This newly developed and validated method allows the rapid and easy determination of the SF2523 concentration with high sensitivity in a low sample volume and can be applied to future pre‐clinical studies.
Carbonic Anhydrase‐Embedded Hydrogen‐Bonded Organic Frameworks Coating for Facilitated Offshore CO2 Fixation
Authors: Zhang Bo‐Yu , Wu Zhen‐Hua , Chu Zi‐Yi , Li Ke , Shi Jia‐Fu 
Published: April 2023

AbstractExhausted emission of carbon dioxide (CO2) from ships or offshore platforms has become one of the major contributors to global carbon emissions. Enzymes such as carbonic anhydrase (CA) have been widely used for CO2 mineralization because of their high catalytic rate. However, CA in seawater is easy to inactivate and difficult to reuse. Immobilization would be a feasible solution to address the stability issue, which, however, may cause an increase of internal diffusion resistance and reduced catalytic activity. In this regard, design of high‐performance biocatalysts for acquiring high catalytic activity and stability of CA is highly desirable. Herein, a monolithic catalyst of Filler‐CA@Lys‐HOF‐1 (FCLH) was prepared by chemical sorption of CA on the surface of the Filler followed by the coating of Lys‐HOF‐1. The highest catalytic activity of FCLH was obtained by regulating the amount of HOF‐1 monomer added. Due to the protection of Lys‐HOF‐1, the FCLH showed good tolerance against acidity and salinity, which could retain about 80.2 % of the original activity after 9 h incubation in simulated seawater. The catalytic activity of FCLH could retain 85.4 % of the initial activity after 10 cycles. Hopefully, our study can provide a promising biocatalyst for CO2 mineralization, which may drive down carbon emissions when used for CO2 capture and conversion on offshore platforms.
Intraoperative ventricular septal rupture: A case report
Authors: Rando Hannah , Kilic Ahmet 
Published: April 2023

AbstractVentricular septal rupture (VSR) is a rare complication of myocardial infarction that requires surgical repair. Herein, we describe a case of intraoperative VSR requiring patch repair and postoperative extracorporeal membrane oxygenation (ECMO) support. This case highlights the risk factors, patient presentation, and management recommendations for this potentially lethal pathology.
An unusual pigmented skin lesion on the nipple‐areola area
Authors: Chaima Kouki , Chaabouni Rim , Bahloul Emna , Boudaya Sonia , Amouri Mariem , Turki Hamida 
Published: April 2023

AbstractConsidering the rarity and underdiagnoses of this disorder, a pigmented and hyperkeratotic skin lesion located on the trunk, resembling to acanthosis nigricans should always be investigated for terra firma‐forme dermatosis (TFFD) and thus alcohol must be applied. TFFD should be known among dermatologists and can be easily diagnosed and treated with isopropyl alcohol.
A case of fatal acute bacterial meningoencephalitis with extremely high cerebrospinal fluid white blood cell count
Authors: AlHodaif Hend , AlOtaibi Sultan , BinSailh Salih , Almuntashri Makki , AlOtaibi Naser , Khatri Ismail A. 
Published: April 2023

AbstractAcute bacterial meningoencephalitis is still prevalent despite the widespread vaccination and still fatal despite the advances in antimicrobial therapy. Identifying patients at risk, lowering the threshold of clinical diagnosis and early treatment of such a curable disease will save patients' lives.
Ultrasound‐guided interventional approach to intraperitoneal nail gun injury: A case report with literature review
Authors: Jalili Javad , Ghane Milad Babazadeh , Azari Morteza , Jafarizadeh Ali , Azari Mojtaba , Falahatian Masih , Alihosseini Samin 
Published: April 2023

AbstractLiterature review suggests that surgery is the only option for dealing with intraperitoneal foreign bodies (laparoscopy and laparotomy). We showed that an interventional method using ultrasound guidance could be considered alongside surgical options.
Insight into the Mechanism of Cd2+ Removal by MgAl Layered Double Hydroxides with Different Host‐Guest Interactions
Authors: Li Qian , Wu Zhaohui , Bai Sha , Liu Yingjie , Li Jiaxin , Song Yu‐Fei 
Published: April 2023

AbstractLayered double hydroxides (LDHs) have shown great potential as adsorbents for the removal of heavy metals. Nevertheless, how the host‐guest interactions of LDHs affect the removal mechanism remains to be less explored. Herein, CO32−/NO3−/SO42−/Cl− intercalated MgAl‐LDHs with different host‐guest interactions were fabricated and their removal mechanism for Cd2+ was investigated. The removal capacity increased in the order of MgAl−CO3 (127.3 mg/g)<MgAl−SO4 (173.3 mg/g)<MgAl−NO3 (305.0 mg/g)≈MgAl−Cl (312.5 mg/g). The quasi‐in‐situ XRD and XAS demonstrated that Cd2+ was removed as CdCO3 for MgAl−CO3, while for MgAl−Cl/NO3/SO4, Cd2+ was removed as CdAl‐LDHs by an isomorphic substitution mechanism. DFT calculations revealed that compared to the CdAl‐LDHs formation the Gibbs free energy of the CdCO3 formation was lower, which made it easier to remove Cd2+ as CdCO3 on MgAl−CO3. Conversely, isomorphic substitution of MgAl−NO3 to obtain CdAl‐LDHs was a free energy reduction process.
Single‐photon emission computed tomography‐defined active bone marrow‐sparing volumetric‐modulated arc therapy reduces the incidence of acute hematologic toxicity in locally advanced cervical cancer patients who receive chemoradiotherapy: A single‐center prospective randomized controlled trial
Authors: Wang ShanBing , Liu Jiapei , Lei Kaijian , Jia Yuming , Wang Chunxiu , Zhang Xia , Li Ting 
Published: April 2023

AbstractBackgroundThis study aims to test the efficacy of single‐photon emission computed tomography (SPECT)‐defined active bone marrow‐sparing (ABMS) volumetric‐modulated arc therapy (VMAT) in reducing grade 3+ acute hematologic toxicity (HT) in locally advanced cervical cancer patients treated with chemoradiotherapy.MethodsThis was a prospective, single‐center, open label, randomized clinical trial that enrolled locally advanced cervical cancer patients. Participants were randomized to the 99mTc sulfur colloid SPECT‐defined ABMS VMAT (ABMS group) or control group, who received weekly cisplatin concurrently with VMAT followed by high–dose‐rate intracavitary brachytherapy. The ABMS group additionally received SPECT‐defined ABM dose constraints. The primary end point was the incidence of grade 3+ acute HT.ResultsA total of 192 Federation of Gynaecology and Obstetrics stage IB‐IIIB patients were randomly treated (96 each in the ABMS control groups). The median follow‐up was 24.0 months. The incidence of grade 3+ acute HT in the ABMS group was significantly lower than that in the control group (32.3% vs. 53.1%, p < .01). The number of patients completing five cycles of cisplatin was 88.5% in the ABMS group and 75% in the control group, and the difference was significant (p = .02). There were no differences in planning target value coverage, organs at risk dosimetric parameters, 2‐year progression‐free survival, or 2‐year overall survival between the two groups. Patients in the control group had nonsignificantly worse 2‐year distant metastasis than patients in the ABMS group (17.8% vs. 11.1%, p = .19).ConclusionsABMS VMAT significantly reduced grade 3+ acute HT and improved chemotherapy delivery compared with the control treatment. We found weak evidence of the effect of ABMS VMAT on distant metastasis.
Antibacterial and Antifouling Properties of Ag‐ and Cu‐Decorated Thin Film Composite Polyamide Reverse Osmosis Membranes
Authors: Anh Ngo Thu Hong , Huy Do Hoang , Thanh Pham Hien , Dinh Do Khai , Dao Duc Sy , Nghia Pham Ngo 
Published: April 2023

AbstractIn this work, silver and copper particles of different nanoparticle precursor concentrations were decorated on the surface of thin film composite polyamide (TFC‐PA) reverse osmosis (RO) membranes by in situ method. The modified membranes were characterized by different techniques including Field emission scanning electron microscopy (FE‐SEM), X‐ray Absorption Spectroscopy (XAS), and attenuated total reflection – Fourier transforms infrared spectroscopy (ATR‐FTIR), water contact angle (WCA), and antibacterial capacity. After that, the membrane performance was compared through normalized flux and the possibility of the removal of bovine serum albumin (BSA) protein; the anti‐fouling and anti‐biofouling properties were also compared through the maintained flux ratios (%) after 5‐hour filtration. The used organic foulants were BSA protein and humic acid (HA) solutions. The results showed that, compared to the flux of the base, the flux of AgNPs‐decorated membrane can improve the separation ability up to 24% while that of CuNPs‐decorated membrane was 14%. The anti‐fouling property of decorated membranes was significantly increased compared to that of the base (from 64% for the base to over 77% for decorated membranes). Especially, the anti‐biofouling property of nanoparticles decorated membranes was also increased because of the higher maintained flux ratios (from 50% for the base to over 75% for decorated membranes). It indicates that this method can be applied to produce reverse osmosis (RO) membranes for reclamation wastewater with a high content of organic matter.
Bleaching‐resistant, Near‐continuous Single‐molecule Fluorescence and FRET Based on Fluorogenic and Transient DNA Binding
Authors: Kümmerlin Mirjam , Mazumder Abhishek , Kapanidis Achillefs N. 
Published: April 2023

AbstractPhotobleaching of fluorescent probes limits the observation span of typical single‐molecule fluorescence measurements and hinders observation of dynamics at long timescales. Here, we present a general strategy to circumvent photobleaching by replenishing fluorescent probes via transient binding of fluorogenic DNAs to complementary DNA strands attached to a target molecule. Our strategy allows observation of near‐continuous single‐molecule fluorescence for more than an hour, a timescale two orders of magnitude longer than the typical photobleaching time of single fluorophores under our conditions. Using two orthogonal sequences, we show that our method is adaptable to Förster Resonance Energy Transfer (FRET) and that can be used to study the conformational dynamics of dynamic structures, such as DNA Holliday junctions, for extended periods. By adjusting the temporal resolution and observation span, our approach enables capturing the conformational dynamics of proteins and nucleic acids over a wide range of timescales.
Fractional mayfly optimization algorithm‐based Infrastructure‐to‐Vehicle and Vehicle‐to‐Vehicle scheduling for service message transmission in IoV‐fog
Authors: Lohat Savita , Jain Sheilza , Kumar Rajender 
Published: April 2023

SummaryFor the rising number of vehicles, and the advancement of communication and computation technologies, the perception of Internet of Vehicles (IoV) is introduced. It is utilized to capture vehicle‐based information that includes road description, road congestion, vehicle speed, and location. However, this information is important, and it showed more benefits in different ways, like route selection and message dissemination. IoV is the self‐structured network composed with vehicles lies in the road and the road side units (RSUs). It offers Infrastructure‐to‐Vehicle (I2V), as well as Vehicle‐to‐Vehicle (V2V) data transmission mechanism for transmitting service messages. To reliably broadcast the service information to the intended recipient in the IoV network still faced issues. Hence, an efficient service message transmission protocol is developed using the proposed fractional mayfly optimization algorithm (FMA) for selecting the relay vehicle and cooperative vehicle for transmitting service messages to the destination vehicle from RSU through the process of I2V and V2V scheduling. The RSU selects the relay vehicle for every service message using the proposed algorithm and allocates the cooperative vehicle by RSU for scheduling V2V transmission. The simulation results showed that the proposed scheduling method obtains the best channel quality indicator (CQI), delay, distance, packet delivery ratio (PDR), and throughput value of 0.92 for 150 vehicles and 0.005891, 6.060731, 83.45%, and 171.50 Mbps for 100 vehicles.
Unraveling the significance of TSHR mutations in indeterminate thyroid cytology specimens
Authors: Andrianus Stefen , Gubbiotti Maria A. , Mandel Susan J. , Nikiforov Yuri E. , Baloch Zubair 
Published: April 2023

AbstractObjectivesWe investigated the clinical significance of thyroid‐stimulating hormone receptor (TSHR) mutations detected in thyroid fine needle aspiration (FNA) specimens.MethodsThe pathology archives at our institution were reviewed between 2018 and 2021 for indeterminate (Bethesda category III and IV) specimens with Thyroseq® analysis showing TSHR mutations.ResultsA total of 2184 cases diagnosed as atypia/follicular lesion of undetermined significance (AUS/FLUS), and 2625 diagnosed as follicular neoplasm/suspicious for follicular neoplasm (FN/SFN) were identified. A total of 1735 AUS/FLUS and 2339 SFN/FN underwent Thyroseq® analysis; 69 showed TSHR mutations (1.6%, 59 female, 10 male, average age: 55 years). Ten cases showed oncocytic features. Twelve patients underwent radionuclide scans within 1 year of FNA:11 were hyperfunctioning. Nodule size and TSH levels were weakly correlated. Twenty‐two different TSHR mutations were identified (most common: M453T). A second mutation was found in five cases (EZH1 n = 2, and EIF1AX n = 3). The expression of sodium‐iodide transporter (NIS) mRNA was in the range of 0.01%–62.43% out of all sequencing reads, and was elevated in 49 (71%) cases. Surgical pathology follow‐up was available in five cases (all benign except one). On follow‐up available for 38 cases (mean: 24 months; range: 7–47 months), 34 (89.5%) nodules remained stable and 3 (8%) increased in size.ConclusionsTSHR mutations in thyroid FNA samples classified as indeterminate are rare, generally benign, and commonly associated with autonomy on scan if performed.
Fast changes in default and control network activity underlying intraindividual response time variability in childhood: Does age and sex matter?
Authors: Privodnova Evgeniya Yu. , Slobodskaya Helena R. , Savostyanov Alexander N. , Bocharov Andrey V. , Saprigyn Alexander E. , Knyazev Gennady G. 
Published: April 2023

AbstractIntraindividual response time variability (RTV) is considered as a general marker of neurological health. In adults, the central executive and salience networks (task‐positive networks, TPN) and the default mode network (DMN) are critical for RTV. Given that RTV decreases with growing up, and that boys are likely somewhat behind girls with respect to the network development, we aimed to clarify age and sex effects. Electroencephalogram was recorded during Stroop‐like test performance in 124 typically developing children aged 5–12 years. Network fluctuations were calculated as changes of current source density (CSD) in regions of interest (ROIs) from pretest to 1‐s test interval. In boys, TPN activation (CSD increase in ROIs included in the TPN) was associated with lower RTV, suggesting a greater engagement of attentional control. In children younger than 9.5 years, higher response stability was associated with the predominance of TPN activation over DMN activation (CSD increase in ROIs included in the TPN > that in the DMN); this predominance increased with age, suggesting that variability among younger children may be due to network immaturity. These findings suggest that the TPN and DMN may play different roles within the network mechanisms of RTV in boys and girls and at different developmental stages.
Is economic hardship associated with young children's cortisol levels?
Authors: Santaularia N. Jeanie , Kunin‐Batson Alicia , Sherwood Nancy E. , Gunnar Megan R. , French Simone A. , Mason Susan M. 
Published: April 2023

AbstractEconomic hardship during childhood has been linked to poor physical and mental health. This study examines cross‐sectional and longitudinal associations of a summed economic hardship score of poverty, food insecurity, and financial hardship with hair cortisol in young children. Data from 24‐month (Time 1, mean age 5 years) and 36‐month (Time 2, mean age 6 years) follow‐up from the NET‐Works obesity prevention trial (NET‐Works, NCT0166891) were used. Hair cortisol measures obtained at each time point were log‐transformed and regressed on economic hardship at Time 1 and a cumulative economic hardship from Time 1 to Time 2, using generalized linear regressions. All models were adjusted for child age, sex, race/ethnicity, and intervention (prevention vs. control) arm. The final analytic sample sizes ranged from 248 to 287. Longitudinal analyses indicated that for every 1‐unit higher economic hardship score at Time 1, hair cortisol at Time 2 follow‐up was on average 0.07 log‐picograms per milligram (pg/mg) higher (95% confidence interval [CI]: 0.01, 0.13). For every 1‐unit increase in the cumulative economic hardship score between Time 1 and 2, there was a 0.04 log‐pg/mg (95% CI: 0.00, 0.07) average higher level of hair cortisol at Time 2 follow‐up. Results show suggestive but limited evidence for an association between economic hardship and cortisol in young children.