bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of Four Cucumber Germplasm Genotypes, Grown in Qatar and the Indian Agro-climatic Zone, Based on Its Phenotypic Traits
Authors: Khan Muvin , Garg Amar Prakash , Jain Sourabh , Tanushiri . 
Published: May 2023

This study included four genotypes of cucumber (IC257296, IC420405, GYNE-5, PUSA SANYOG) which were evaluated based on phenotypic traits when grown under two different soil zones of Doha (Qatar) and Meerut (India). Statistical analysis to estimate various genetic parameters like phenotypic and genotypic variance, phenotypic and genotypic coefficient of variation (PCV and GCV), broad sense heritability and genetic gain and principal component analysis (PCA) of genotypes was done in order to assess the magnitude of variability for various agro-metrical characters. In Indian soil zone, highest yield was observed in GYNE-5, while, in Qatar soil zone, highest yield was observed in PUSA SANYOG. The phenotypic coefficient of variation (PCV) was higher than its corresponding genotypic counterpart (GCV) for all characters studied. High broad-sense heritability was associated with all the traits in both zones except fruit length. Days to edible maturity, fruit length and fruit width showed low genetic gain in qatar zone; hence, heterosis breeding would be recommended. Principal component analysis involved vine length and days to edible maturity as the most discriminating trait that accounted for greater variability in cucumber in qatar zone, and it should be considered in cucumber improvement programs. Correlation analysis represent that yield was positively correlated with fruit length and fruit width, while, fruit per plant showed positive significant correlation with vine length. Present findings suggest that PUSA SANYOG may be cultivated in other areas with similar climatic conditions. This study will help to improve the protected agriculture model of Qatar and revenue generation for the farmers.
Cost-effectiveness of Increasing Buprenorphine Treatment Initiation, Duration, and Capacity Among Individuals Who Use Opioids
Authors: Claypool Anneke L. , DiGennaro Catherine , Russell W. Alton , Yildirim Melike F. , Zhang Alan F. , Reid Zuri , Stringfellow Erin J. , Bearnot Benjamin , Schackman Bruce R. , Humphreys Keith , Jalali Mohammad S. 
Published: May 2023

ImportanceBuprenorphine is an effective and cost-effective medication to treat opioid use disorder (OUD), but is not readily available to many people with OUD in the US. The current cost-effectiveness literature does not consider interventions that concurrently increase buprenorphine initiation, duration, and capacity.ObjectiveTo conduct a cost-effectiveness analysis and compare interventions associated with increased buprenorphine treatment initiation, duration, and capacity.Design and SettingThis study modeled the effects of 5 interventions individually and in combination using SOURCE, a recent system dynamics model of prescription opioid and illicit opioid use, treatment, and remission, calibrated to US data from 1999 to 2020. The analysis was run during a 12-year time horizon from 2021 to 2032, with lifetime follow-up. A probabilistic sensitivity analysis on intervention effectiveness and costs was conducted. Analyses were performed from April 2021 through March 2023. Modeled participants included people with opioid misuse and OUD in the US.InterventionsInterventions included emergency department buprenorphine initiation, contingency management, psychotherapy, telehealth, and expansion of hub-and-spoke treatment programs, individually and in combination.Main Outcomes and MeasuresTotal national opioid overdose deaths, quality-adjusted life years (QALYs) gained, and costs from the societal and health care perspective.ResultsProjections showed that contingency management expansion would avert 3530 opioid overdose deaths over 12 years, more than any other single-intervention strategy. Interventions that increased buprenorphine treatment duration initially were associated with an increased number of opioid overdose deaths in the absence of expanded treatment capacity. With an incremental cost- effectiveness ratio of $19 381 per QALY gained (2021 USD), the strategy that expanded contingency management, hub-and-spoke training, emergency department initiation, and telehealth was the preferred strategy for any willingness-to-pay threshold from $20 000 to $200 000/QALY gained, as it was associated with increased treatment duration and capacity simultaneously.Conclusion and RelevanceThis modeling analysis simulated the effects of implementing several intervention strategies across the buprenorphine cascade of care and found that strategies that were concurrently associated with increased buprenorphine treatment initiation, duration, and capacity were cost-effective.
Effect of Vitamin D3 Supplementation in the First 2 Years of Life on Psychiatric Symptoms at Ages 6 to 8 Years
Authors: Sandboge Samuel , Räikkönen Katri , Lahti-Pulkkinen Marius , Hauta-alus Helena , Holmlund-Suila Elisa , Girchenko Polina , Kajantie Eero , Mäkitie Outi , Andersson Sture , Heinonen Kati 
Published: May 2023

ImportanceVitamin D is associated with neurodevelopment, but causality, critical windows, and potentials for modification remain unknown.ObjectiveTo determine the impact of high-dose (1200 IU) vs standard-dose (400 IU) vitamin D3 supplementation during the first 2 years on psychiatric symptoms at ages 6 to 8 years and whether the impact is different in children with lower vs higher maternal vitamin D3 levels; lower vs higher levels were defined as 25-hydroxyvitamin D (25[OH]D) less than 30 ng/mL vs 30 ng/mL or greater.Design, Setting, and ParticipantsThis study was a long-term follow-up of the double-blind randomized clinical trial (RCT) Vitamin D Intervention in Infants (VIDI) conducted at a single center in Helsinki, Finland, at 60 degrees north latitude. Recruitment for VIDI took place in 2013 to 2014. Follow-up data for secondary data analysis were collected 2020 to 2021. VIDI originally included 987 term-born infants; 546 of these individuals participated in the follow-up at ages 6 to 8 years, among whom 346 individuals had data on parent-reported psychiatric symptoms. Data were analyzed from June 2022 to March 2023.InterventionsThere were 169 infants randomized to receive 400-IU and 177 infants randomized to receive 1200-IU oral vitamin D3 supplementation daily from ages 2 weeks to 24 months.Main Outcomes and MeasuresPrimary outcomes were internalizing, externalizing, and total problems scores, with clinically significant problems defined as T scores of 64 or greater in the Child Behavior Checklist questionnaire.ResultsAmong 346 participants (164 females [47.4%]; mean [SD] age, 7.1 [0.4] years), the vitamin D3 dose was 400 IU for 169 participants and 1200 IU for 177 participants. Clinically significant internalizing problems occurred in 10 participants in the 1200-IU group (5.6% prevalence) compared with 20 participants (11.8%) in the 400-IU group (odds ratio, 0.40; 95% CI, 0.17-0.94; P = .04) after adjustment for sex, birth season, maternal depressive symptoms at birth, and parental single status at follow-up. In a post hoc subgroup analysis, 48 children in the 400-IU group with maternal 25(OH)D concentrations less than 30 ng/mL had higher internalizing problems scores compared with children in the 1200-IU group, including 44 children with maternal 25(OH)D concentrations below 30 ng/mL (adjusted mean difference, 0.49; 95% CI, 0.09-0.89; P = .02) and 91 children with maternal concentrations above 30 ng/mL (adjusted mean difference, 0.37; 95% CI, 0.03-0.72; P = .04). Groups did not differ in externalizing or total problems.Conclusions and RelevanceThis randomized clinical trial found that higher-than-standard vitamin D3 supplementation in the first 2 years decreased risk of internalizing problems at ages 6 to 8 years.Trial RegistrationClinicalTrials.gov Identifiers: NCT01723852 (VIDI) and NCT04302987 (VIDI2)
Acute and Repeated Ozone Exposures Differentially Affect Circadian Clock Gene Expression in Mice
Authors: Sundar Isaac Kirubakaran , Duraisamy Santhosh Kumar , Choudhary Ishita , Saini Yogesh , Silveyra Patricia 
Published: May 2023

AbstractCircadian rhythms have an established role in regulating physiological processes, such as inflammation, immunity, and metabolism. Ozone, a common environmental pollutant with strong oxidative potential, is implicated in lung inflammation/injury in asthmatics. However, whether O3 exposure affects the expression of circadian clock genes in the lungs is not known. In this study, changes in the expression of core clock genes are analyzed in the lungs of adult female and male mice exposed to filtered air (FA) or O3 using qRT‐PCR. The findings are confirmed using an existing RNA‐sequencing dataset from repeated FA‐ and O3‐exposed mouse lungs and validated by qRT‐PCR. Acute O3 exposure significantly alters the expression of clock genes in the lungs of females (Per1, Cry1, and Rora) and males (Per1). RNA‐seq data revealing sex‐based differences in clock gene expression in the airway of males (decreased Nr1d1/Rev‐erbα) and females (increased Skp1), parenchyma of females and males (decreased Nr1d1 and Fbxl3 and increased Bhlhe40 and Skp1), and alveolar macrophages of males (decreased Arntl/Bmal1, Per1, Per2, Prkab1, and Prkab2) and females (increased Cry2, Per1, Per2, Csnk1d, Csnk1e, Prkab2, and Fbxl3). These findings suggest that lung inflammation caused by O3 exposure affects clock genes which may regulate key signaling pathways.
Sequential‐Stimuli Induced Stepwise‐Response of Pyridylpyrenes
Authors: Zhang Yufeng , Zhang Shuai , Liang Chen , Shi Junqing , Ji Lei 
Published: May 2023

AbstractStimuli‐responsive materials, especially multi‐stimuli‐responsive materials, can sense external stimuli such as light, heat, and force, have shown great potential in drug delivery, data storage, encryption, energy‐harvesting, and artificial intelligence. Conventional multi‐stimuli‐responsive materials are sensitive to each independent stimulus, causing losses in the diversity and accuracy of the identification for practical application. Herein, a unique phenomenon of sequential‐stimuli induced stepwise‐response generated from elaborately designed single‐component organic materials is reported, which shows large bathochromic shifts up to 5800 cm−1 under sequential stimuli of force and light. In contrast to multi‐stimuli‐responsive materials, the response of these materials strictly relies on the sequence of stimuli, allowing logicality, rigidity, and accuracy to be integrated into one single‐component material. The molecular keypad lock is built based on these materials, pointing promising to a future for this logical response in significant practical applications. This breakthrough gives a new drive to classical stimuli‐responsiveness and provides a fundamental design strategy for new generations of high‐performance stimuli‐responsive materials.
Role of Potassium in Electrocatalytic Water Oxidation Investigated in a Volume‐Active Cobalt Material at Neutral pH
Authors: Liu Si , Farhoosh Shima , Beyer Paul , Mebs Stefan , Zaharieva Ivelina , Haumann Michael , Dau Holger 
Published: May 2023

AbstractThe oxygen evolution reaction (OER) is crucial in systems for sustainable production of hydrogen and other fuels. Catalytic OER materials often undergo potential‐induced redox transitions localized at metal sites. For volume‐active catalyst‐materials, these are necessarily coupled to charge‐compensating relocation of ions entering or leaving the material, which is insufficiently understood. The binding mode and mechanistic role of redox‐inert ions for a cobalt‐based oxyhydroxide material (CoCat) when operated at neutral pH in potassium‐phosphate (KPi) electrolyte are investigated by i) determination of K:Co and P:Co stoichiometries for various KPi‐concentrations and electrode potentials, ii) operando X‐ray spectroscopy at the potassium and cobalt K‐edges, and iii) novel time‐resolved X‐ray experiments facilitating comparison of K‐release and Co‐oxidation kinetics. Potassium likely binds non‐specifically within water layers interfacing Co‐oxyhydoxide fragments involving potassium–phosphate ion pairs. The potassium‐release kinetics are potential‐independent with a fast‐phase time‐constant of about 5 s and thus clearly slower than the potential‐induced Co oxidation of about 300 ms. It is concluded that the charge‐compensating ion flow is realized neither by potassium nor by phosphate ions, but by protons. The results reported here are likely relevant also for a broader class of volume‐active OER catalyst materials and for the amorphized near‐surface regions of microcrystalline materials.
Remodeling Serine Synthesis and Metabolism via Nanoparticles (NPs)‐Mediated CFL1 Silencing to Enhance the Sensitivity of Hepatocellular Carcinoma to Sorafenib
Authors: Li Senlin , Xu Lei , Wu Guo , Huang Ziqi , Huang Linzhuo , Zhang Fengqian , Wei Chunfang , Shen Qian , Li Rong , Zhang Lei , Xu Xiaoding 
Published: May 2023

AbstractTyrosine kinase inhibitors represented by sorafenib are the first‐line treatment for hepatocellular carcinoma (HCC), but the low response rate of HCC patient has become a clinical pain‐point. Emerging evidences have revealed that metabolic reprogramming plays an important role in regulating the sensitivity of tumor cells to various chemotherapeutics including sorafenib. However, the underlying mechanisms are very complex and are not fully elucidated. By comparing the transcriptome sequencing data of sorafenib‐sensitive and ‐insensitive HCC patients, it is revealed that cofilin 1 (CFL1) is highly expressed in the tumor tissues of sorafenib‐insensitive HCC patients and closely correlated with their poor prognosis. Mechanically, CFL1 can promote phosphoglycerate dehydrogenase transcription and enhance serine synthesis and metabolism to accelerate the production of antioxidants for scavenging the excessive reactive oxygen species induced by sorafenib, thereby impairing the sorafenib sensitivity of HCC. To translate this finding and consider the severe side effects of sorafenib, a reduction‐responsive nanoplatform for systemic co‐delivery of CFL1 siRNA (siCFL1) and sorafenib is further developed, and its high efficacy in inhibiting HCC tumor growth without apparent toxicity is demonstrated. These results indicate that nanoparticles‐mediated co‐delivery of siCFL1 and sorafenib can be a new strategy for the treatment of advanced HCC.
Locoregional treatment for colorectal liver metastases aiming for precision medicine
Authors: Maki Harufumi , Jain Anish J. , Haddad Antony , Lendoire Mateo , Chun Yun Shin , Vauthey Jean‐Nicolas 
Published: May 2023

AbstractIn patients with colorectal liver metastases (CLM), surgery is potentially curative. The use of novel surgical techniques and complementary percutaneous ablation allows for curative‐intent treatment even in marginally resectable cases. Resection is used as part of a multidisciplinary approach, which for nearly all patients will include perioperative chemotherapy. Small CLM can be treated with parenchymal‐sparing hepatectomy (PSH) and/or ablation. For small CLM, PSH results in better survival and higher rates of resectability of recurrent CLM than non‐PSH. For patients with extensive bilateral distribution of CLM, two‐stage hepatectomy or fast‐track two‐stage hepatectomy is effective. Our increasing knowledge of genetic alterations allows us to use them as prognostic factors alongside traditional risk factors (e.g. tumor diameter and tumor number) to select patients with CLM for resection and guide surveillance after resection. Alteration in RAS family genes (hereafter referred to as “RAS alteration”) is an important negative prognostic factor, as are alterations in the TP53, SMAD4, FBXW7, and BRAF genes. However, APC alteration appears to improve prognosis. RAS alteration, increased number and diameter of CLM, and primary lymph node metastasis are well‐known risk factors for recurrence after CLM resection. In patients free of recurrence 2 y after CLM resection, only RAS alteration is associated with recurrence. Thus, surveillance intensity can be stratified by RAS alteration status after 2 y. Novel diagnostic instruments and tools, such as circulating tumor DNA, may lead to further evolution of patient selection, prognostication, and treatment algorithms for CLM.
Copper/Photoredox dual Catalyzed Syntheses of Allenenitriles via 1,5‐HAT
Authors: Li Yuan‐Bo , Bi Yu‐Xiang , Ke Wen‐Chang , Wang Cheng‐Yu , Hu Duo‐Duo , Li Yan , Wang Xi‐Sheng 
Published: May 2023

AbstractThe first example of merging photoredox catalysis and copper catalysis for 1,4‐difunctionalization of 1,3‐enynes via 1,5‐HAT (hydrogen atom transfer) has been developed. The synthetic strategy exploits a radical translocation from an oxygen‐centered to a carbon‐centered radical with a subsequent radical addition to the 1,3‐enynes, affording tetrasubstituted allenenitriles in up to 90% yield. This strategy has mild reaction conditions, broad substrate scope and excellent functional group tolerance.
Perilipin 5 regulates hepatic stellate cell activation and high‐fat diet‐induced non‐alcoholic fatty liver disease
Authors: Yin Xuecui , Dong Lin , Wang Xiaohan , Qin Zhenzhen , Ma Yuying , Ke Xiaofei , Li Ya , Wang Qingde , Mi Yang , Lyu Quanjun , Xu Xia , Zheng Pengyuan , Tang Youcai 
Published: May 2023

AbstractBackgroundNonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases globally. Hepatic stellate cells (HSCs) are the major effector cells of liver fibrosis. HSCs contain abundant lipid droplets (LDs) in their cytoplasm during quiescence. Perilipin 5 (PLIN 5) is a LD surface‐associated protein that plays a crucial role in lipid homeostasis. However, little is known about the role of PLIN 5 in HSC activation.MethodsPLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus transfection. At the same time, PLIN 5 gene knockout mice were constructed and fed with a high‐fat diet (HFD) for 20 weeks to study the role of PLIN 5 in NAFLD. The corresponding reagent kits were used to measure TG, GSH, Caspase 3 activity, ATP level, and mitochondrial DNA copy number. Metabolomic analysis of mice liver tissue metabolism was performed based on UPLC‐MS/MS. AMPK, mitochondrial function, cell proliferation, and apoptosis‐related genes and proteins were detected by western blotting and qPCR.ResultsOverexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria, inhibition of cell proliferation, and a significant increase in cell apoptosis through AMPK activation. In addition, compared with the HFD‐fed C57BL/6J mice, PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition, decreased LD abundance and size, and reduced liver fibrosis.ConclusionThese findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.
Interlaboratory comparisons of cyanotoxin analysis by ELISA and LC–MS/MS
Authors: Prescott Matthew D. , Jia Ai , Guo Yingbo C. , Di Giovanni George D. , Li Wei L. , Garcia Eduardo A. , Vanderford Brett J. , Eaton Andrew D. 
Published: May 2023

AbstractTwo commonly used methods for cyanotoxin analysis are enzyme‐linked immunosorbent assay (ELISA) and liquid chromatography/tandem mass spectrometry (LC–MS/MS). Two rounds of interlaboratory comparisons of ELISA and LC–MS/MS analyses were conducted with 12 participating laboratories to evaluate method performances in various matrices, including cyanobacterial bloom and drinking water samples. Fifteen cyanotoxins, including 12 microcystin variants, nodularin, anatoxin‐a, and cylindrospermopsin were evaluated. The impact of sample matrices, preservatives, and quenching reagents was assessed, and no substantial effects were observed. Overall, comparable results were obtained among laboratories performing ELISA and LC–MS/MS analyses, respectively. ELISA results for fortified samples matched more closely with those from LC–MS/MS when microcystin cross‐reactivities were considered, providing data 26% closer to theoretical values on average. This study demonstrates that understanding the effect of cross‐reactivities when comparing ELISA and LC–MS/MS results and considering potential variabilities in commercial standards is important when interpreting data from these two methods.
The Use of Reductive Agents for Developing Capacity Balanced Aqueous Sodium‐Ion Batteries
Authors: Petrulevičienė Milda , Traškina Nadežda , Pilipavičius Jurgis , Juodkazytė Jurga , Vilčiauskas Linas 
Published: May 2023

AbstractAqueous sodium‐ion batteries (Na‐ion batteries) represent an attractive low cost stationary energy storage technology. However, limited electrochemical stability of water and issues related to complex aqueous chemistry, component corrosion, and materials dissolution limit their lifetime. Oxygen reduction reaction is considered to be the main parasitic process in aqueous Na‐ion batteries leading to self‐discharge, materials degradation and Na‐ion inventory loss. Herein, we present a comprehensive study on novel electrolyte design which allows to mitigate the main parasitic reactions and prepare capacity balanced (N/P=1) aqueous battery cells. For this purpose, aqueous symmetric Na2VTi(PO4)3|Na2VTi(PO4)3 system is used as a model to demonstrate that the introduction of a small concentration of strongly reducing agent such as hydrazine into purely aqueous or hybrid (water and dimethyl sulphoxide) electrolyte could chemically reduce the dissolved oxygen and significantly improve the capacity retention of N/P=1 cells during cycling. The low concentrations and self‐consuming nature of hydrazine in a closed cell do not pose any health or chemical risks and presents a viable strategy for practical design. We believe, these results to be applicable not only to aqueous Na‐ion batteries but also other aqueous or hybrid battery chemistries where such parasitic processes play a significant role.
Engineering single‐domain antibodies targeting Gasdermin E activation by the chemotherapeutic agent cis‐diaminodichloroplatinum
Authors: Ma Jinyi , Xu Jintao , Gao Qiuyun , Sun Yanan , Wang Yu , Liu Zhe , Ma Zhenyi 
Published: May 2023

AbstractAs mediators of pyroptosis, gasdermins (GSDMs) are closely associated with systemic cytotoxicity or so‐called side effects and are also involved in the inflammatory response during chemotherapy. Using in situ proximity ligation assay followed by sequencing (isPLA‐seq), which we recently developed, we screened a single‐domain antibody (sdAb) library and identified several sdAbs against Gasdermin E (GSDME) that specifically recognize the N‐terminal domain (1‐270 aa) of GSDME (GSDME‐NT). One of them mitigated the release of inflammatory damage‐associated molecular patterns (DAMPs) and cytokines, including high mobility group protein b1 (Hmgb1) and interleukin‐1β (Il‐1β), in isolated mouse alveolar epithelial cells (AECs) upon chemotherapeutic agent cis‐diaminodichloroplatinum (CDDP) treatment. Further investigation showed that this anti‐GSDME sdAb also alleviated CDDP‐induced pyroptotic cell death and lung tissue injury and decreased systemic Hmgb1 release in C57/BL6 mice, due to GSDME inactivation. Collectively, our data define an inhibitory role of the specific sdAb against GSDME, providing a potential strategy for systemically alleviating chemotherapeutic toxicities in vivo.