bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generation of rat offspring using spermatids produced through in vitro spermatogenesis
Authors: Matsumura Takafumi , Katagiri Kumiko , Yao Tatsuma , Ishikawa-Yamauchi Yu , Nagata Shino , Hashimoto Kiyoshi , Sato Takuya , Kimura Hiroshi , Shinohara Takashi , Sanbo Makoto , Hirabayashi Masumi , Ogawa Takehiko 
Published: July 2023

AbstractAn in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture method has generally not been effective in other animal species, we recently succeeded in improving the culture condition to induce spermatogenesis of rats up to the round spermatid stage. In the present study, we introduced acrosin-EGFP transgenic rats in order to clearly monitor the production of haploid cells during spermatogenesis in vitro. In addition, a metabolomic analysis of the culture media during cultivation revealed the metabolic dynamics of the testis tissue. By modifying the culture media based on these results, we were able to induce rat spermatogenesis repeatedly up to haploid cell production, including the formation of elongating spermatids, which was confirmed histologically and immunohistochemically. Finally, we performed a microinsemination experiment with in vitro produced spermatids, which resulted in the production of healthy and fertile offspring. This is the first demonstration of the in vitro production of functional haploid cells that yielded offspring in animals other than mice. These results are expected to provide a basis for the development of an in vitro spermatogenesis system applicable to many other mammals.
Comparative study of TiO2–Fe3O4 photocatalysts synthesized by conventional and microwave methods for metronidazole removal
Authors: Kubiak Adam 
Published: July 2023

AbstractThis study focused on a direct comparison of conventional hydrothermal and microwave treatment during the synthesis of TiO2–Fe3O4photocatalyst, which is an effective catalyst for decomposing metronidazole. The photocatalyst underwent various characterization analyses, including X-ray diffraction, Raman spectroscopy, transmission electron microscopy, energy dispersive X-ray, and diffuse reflectance spectroscopy. The Raman spectroscopy analysis revealed that the materials obtained through the conventional hydrothermal treatment consisted of separate phases of anatase and magnetite. On the other hand, the materials synthesized using the microwave process showed a noticeable shift in the Egband (143 cm−1) and its half-width towards higher wavenumbers. This shift is likely due to the introduction of Fe ions into the TiO2lattice. Additionally, both conventional hydrothermal and microwave synthesis routes produced TiO2–Fe3O4systems with superparamagnetic properties, as demonstrated by SQUID magnetic measurements. The TEM analysis revealed that the materials synthesized using the microwave process exhibited higher homogeneity, with no noticeable large aggregates observed. Finally, this work proposed a convenient LED photoreactor that effectively utilized the photo-oxidative properties of TiO2–Fe3O4photocatalysts to remove metronidazole. Combining photoactive TiO2–Fe3O4catalysts with an energy-efficient LED reactor resulted in a low electrical energy per order (EEO).
Machine-learning-based predictions of imprinting quality using ensemble and non-linear regression algorithms
Authors: Yarahmadi Bita , Hashemianzadeh Seyed Majid , Milani Hosseini Seyed Mohammad-Reza 
Published: July 2023

AbstractThe molecularly imprinted polymers are artificial polymers that, during the synthesis, create specific sites for a definite purpose. These polymers due to their characteristics such as stability, easy of synthesis, reproducibility, reusability, high accuracy, and selectivity have many applications. However, the variety of the functional monomers, templates, solvents, and synthesis conditions like pH, temperature, the rate of stirring, and time, limit the selectivity of imprinting. The Practical optimization of the synthetic conditions has many drawbacks, including chemical compound usage, equipment requirements, and time costs. The use of machine learning (ML) for the prediction of the imprinting factor (IF), which indicates the quality of imprinting is a very interesting idea to overcome these problems. The ML has many advantages, for example a lack of human error, high accuracy, high repeatability, and prediction of a large amount of data in the minimum time. In this research, ML was used to predict the IF using non-linear regression algorithms, including classification and regression tree, support vector regression, and k-nearest neighbors, and ensemble algorithms, like gradient boosting (GB), random forest, and extra trees. The data sets were obtained practically in the laboratory, and inputs, included pH, the type of the template, the type of the monomer, solvent, the distribution coefficient of the MIP (KMIP), and the distribution coefficient of the non-imprinted polymer (KNIP). The mutual information feature selection method was used to select the important features affecting the IF. The results showed that the GB algorithm had the best performance in predicting the IF, and using this algorithm, the maximum R2 value (R2 = 0.871), and the minimum mean absolute error (MAE = − 0.982), and mean square error were obtained (MSE = − 2.303).
An intranasal vaccine comprising SARS-CoV-2 spike receptor-binding domain protein entrapped in mannose-conjugated chitosan nanoparticle provides protection in hamsters
Authors: Tabynov Kairat , Solomadin Maxim , Turebekov Nurkeldi , Babayeva Meruert , Fomin Gleb , Yadagiri Ganesh , Renu Sankar , Yerubayev Toktassyn , Petrovsky Nikolai , Renukaradhya Gourapura J. , Tabynov Kaissar 
Published: July 2023

AbstractWe developed a novel intranasal SARS-CoV-2 subunit vaccine called NARUVAX-C19/Nano based on the spike protein receptor-binding domain (RBD) entrapped in mannose-conjugated chitosan nanoparticles (NP). A toll-like receptor 9 agonist, CpG55.2, was also added as an adjuvant to see if this would potentiate the cellular immune response to the NP vaccine. The NP vaccine was assessed for immunogenicity, protective efficacy, and ability to prevent virus transmission from vaccinated animals to naive cage-mates. The results were compared with a RBD protein vaccine mixed with alum adjuvant and administered intramuscularly. BALB/c mice vaccinated twice intranasally with the NP vaccines exhibited secretory IgA and a pronounced Th1-cell response, not seen with the intramuscular alum-adjuvanted RBD vaccine. NP vaccines protected Syrian hamsters against a wild-type SARS-CoV-2 infection challenge as indicated by significant reductions in weight loss, lung viral load and lung pathology. However, despite significantly reduced viral load in the nasal turbinates and oropharyngeal swabs from NP-vaccinated hamsters, virus transmission was not prevented to naïve cage-mates. In conclusion, intranasal RBD-based NP formulations induced mucosal and Th1-cell mediated immune responses in mice and protected Syrian hamsters against SARS-CoV-2 infection but not against viral transmission.
Synthesis of N-oxyamide analogues of protein kinase B (Akt) targeting anionic glycoglycerolipids and their antiproliferative activity on human ovarian carcinoma cells
Authors: Zuccolo Marco , Orsini Giulia , Quaglia Martina , Mirra Luca , Corno Cristina , Carenini Nives , Perego Paola , Colombo Diego 
Published: July 2023

N-Oxyamides of bioactive anionic glycoglycerolipids based on 2-O-β-d-glucosylglycerol were efficiently prepared. The N-oxyamide group could increase the antiproliferative activity of glycoglycerolipids.
Increased stiffness of omental arteries from late pregnant women at advanced maternal age
Authors: Wooldridge Amy L. , Chan Christy , Spaans Floor , Quon Anita , Steinback Craig D. , Davenport Margie H. , Davidge Sandra T. , Cooke Christy-Lynn M. 
Published: July 2023

Abstract
Advanced maternal age (≥35 years) is a risk factor for poor pregnancy outcomes. Pregnancy requires extensive maternal vascular adaptations, and with age, our blood vessels become stiffer and change in structure (collagen and elastin). However, the effect of advanced maternal age on the structure of human resistance arteries during pregnancy is unknown. As omental resistance arteries contribute to blood pressure regulation, assessing their structure in pregnancy may inform on the causal mechanisms underlying pregnancy complications in women of advanced maternal age. Omental fat biopsies were obtained from younger (&lt;35 years) or advanced maternal age (≥35 years) women during caesarean delivery (n = 7–9/group). Arteries (200–300 µm) were isolated and passive mechanical properties (circumferential stress and strain) assessed with pressure myography. Collagen (Masson’s Trichrome) and elastin (Verhoff) were visualized histologically and % positively-stained area was assessed. Median maternal age was 32 years (range 25–34) for younger, and 38 years (range 35–42) for women of advanced maternal age. Circumferential strain was lower in arteries from advanced maternal age versus younger women but circumferential stress was not different. Omental artery collagen levels were similar, while elastin levels were lower with advanced maternal age versus younger pregnancies. The collagen:elastin ratio was greater in arteries from advanced maternal age versus younger women. In conclusion, omental arteries from women of advanced maternal age were less compliant with less elastin compared with arteries of younger controls, which may affect how vascular stressors are tolerated during pregnancy. Understanding how vascular aging affects pregnancy adaptations may contribute to better pregnancy outcomes.
Why Are Causal and Temporal Connectives Difficult to Understand? A Comparison Between Italian Hearing Good and Poor Comprehenders and Deaf Poor Comprehenders
Authors: Arfé Barbara , Carretti Barbara , van den Broek Paul , Benincasa Paola , Genovese Elisabetta , Oakhill Jane 
Published: July 2023

Purpose:
Both hearing poor comprehenders (PCs) and deaf or hard-of-hearing (DHH) PCs have problems in understanding causal and temporal coherence relations signaled by connectives. The study examined whether hearing and DHH PCs' problems with connective understanding are similar and mainly related to their limited vocabulary, including knowledge of connective words, or to their poor reading comprehension abilities more generally.

Method:
Three groups of 7- to 10-year-old readers, matched on grade level (hearing PCs, DHH PCs, and hearing good comprehenders [GCs]) performed a reading comprehension task, a vocabulary task, and causal and temporal connective understanding tasks. Hearing and DHH PCs were also matched on reading comprehension and decoding abilities.

Results:
The DHH PCs performed significantly worse than both the hearing GCs and PCs in temporal and causal connective understanding. Significant differences between hearing PCs and GCs were found only in causal connective understanding. DHH readers' difficulties in causal connective understanding were significantly associated with poorer vocabulary knowledge. In contrast, vocabulary knowledge did not uniquely contribute to hearing PCs' difficulties with causal connective understanding, once their reading comprehension skills were controlled for.

Conclusions:
The results suggest that despite a similar reading profile, DHH PCs' difficulties with causal connective understanding are more closely related to their vocabulary delay, whereas hearing PCs' difficulties are more strongly influenced by their poor text integration processes (as indexed by their reading comprehension skills). Neither vocabulary knowledge nor reading comprehension skills contributed to the explanation of DHH readers and hearing PCs' temporal connective understanding.
Diadochokinesis Performance and Its Link to Cognitive Control: Alternating Versus Non-Alternating Diadochokinesis
Authors: Janse Esther , Shen Chen , de Kerf Esther 
Published: July 2023

Purpose:

In a previous publication, we observed that maximum speech performance in a nonclinical sample of young adult speakers producing
alternating
diadochokinesis (DDK) sequences (e.g., rapidly repeating “pataka”) was associated with cognitive control: Those with better cognitive switching abilities (i.e., switching flexibly between tasks or mental sets) showed higher DDK accuracy. To follow up on these results, we investigated whether this previously observed association is specific to the rapid production of
alternating
sequences or also holds for
non-alternating
sequences (e.g., “tatata”).

Method:
For the same sample of 78 young adults as in our previous study, we additionally analyzed their accuracy and rate performance on non-alternating sequences to investigate whether executive control abilities (i.e., indices of speakers' updating, inhibition, and switching abilities) were more strongly associated with production of alternating, as compared with non-alternating, sequences.

Results:

Of the three executive control abilities, only switching predicted both DDK accuracy and rate. The association between cognitive switching (and updating ability) and DDK
accuracy
was only observed for alternating sequences. The DDK
rate
model included a simple effect of cognitive switching, such that those with better switching ability showed slower diadochokinetic rates across the board. Thus, those with better cognitive ability showed more accurate (alternating) diadochokinetic production and slower maximum rates for both alternating and non-alternating sequences.

Conclusion:
These combined results suggest that those with better executive control have better control over their maximum speech performance and show that the link between cognitive control and maximum speech performance also holds for non-alternating sequences.
Analysis of Needlet Internal Linear Combination performance on B-mode data from sub-orbital experiments
Authors: Carones Alessandro , Migliaccio Marina , Marinucci Domenico , Vittorio Nicola 
Published: July 2023

Context. The observation of primordial B modes in cosmic microwave background (CMB) polarisation data represents the main scientific goal of most of the future CMB experiments. This signal is predicted to be much lower than polarised Galactic emission (foregrounds) in any region of the sky, pointing to the need for effective component separation methods.
Aims. Among all the techniques, the blind Needlet Internal Linear Combination (NILC) is of great relevance given our current limited knowledge of the B-mode foregrounds. In this work, we explore the possibility of employing NILC for the analysis of B modes reconstructed from partial-sky data, specifically addressing the complications that such an application yields such as E–B leakage, needlet filtering, and beam convolution.
Methods. We consider two complementary simulated datasets of future experiments: the balloon-borne Short Wavelength Instrument for the Polarisation Explorer (SWIPE) of the Large Scale Polarisation Explorer, which targets the observation of both reionisation and recombination peaks of the primordial CMB B-mode angular power spectrum, and the ground-based Small Aperture Telescope of Simons Observatory, which, instead, is designed to observe only the recombination bump at ℓ ∼ 80. We assessed the performance of the following two alternative techniques to correct for the CMB E–B leakage: the recycling technique and the Zhao-Baskaran method.
Results. We find that both techniques reduce the E–B leakage residuals at a negligible level given the sensitivity of the considered experiments, except for the recycling method in the SWIPE footprint at ℓ < 20. Thus, we implemented two extensions of the pipeline, the iterative B decomposition and the diffusive inpainting, which enabled us to recover the input CMB B-mode power for ℓ ≥ 5. For the considered experiments, we demonstrate that needlet filtering and beam convolution do not affect the CMB B-mode reconstruction. Finally, with an appropriate masking strategy, we find that NILC foregrounds subtraction allows one to achieve sensitivities on the tensor-to-scalar ratio in agreement with the targets of the considered CMB experiments.
Strukturelle, prozedurale und personelle Voraussetzungen für eine sektorenübergreifende Erbringung endoskopischer gastroenterologischer Leistungen
Authors: Leifeld Ludger , Denzer Ulrike , Frieling Thomas , Jakobs Ralf , Faiss Siegbert , Lenzen Henrike , Lynen Petra , Mayerle Julia , Ockenga Johann , Tappe Ulrich , Terjung Birgit , Wedemeyer Heiner , Albert Jörg ,   für die DGVS
Published: July 2023

ZusammenfassungDie Gastroenterologie hat entscheidende Fortschritte in diagnostischen und interventionellen endoskopischen Verfahren gemacht, die eine Verbesserung in der Behandlung von vielen Patienten eröffnet. So werden zunehmend organerhaltende Behandlungen ermöglicht, die invasivere organresezierende chirurgische Verfahren ersetzen. Dabei ist das Ausmaß an Komplexität und an Risiken zwischen den verschiedenen endoskopischen Prozeduren sehr unterschiedlich. Vielfach werden heute einfachere endoskopische Prozeduren ambulant angeboten. Weiteres Potenzial einer sektorenübergreifenden Leistungserbringung besteht bei komplexen endoskopischen Eingriffen, die aber besondere strukturelle, prozedurale und personelle Voraussetzungen bedingen, um qualitätsgesichert die Behandlung zu erbringen, eine post-interventionelle Überwachung zu ermöglichen und ggf. Maßnahmen für eine Sicherung des Behandlungserfolgs zu ergreifen. Wir fassen die wesentlichen Voraussetzungen und Grenzen für eine sektorenübergreifende Durchführung endoskopischer Prozeduren in der Gastroenterologie zusammen.
Clinical Implications of Discrepancy between One-Stage Clotting and Chromogenic Factor IX Activity in Hemophilia B
Authors: Schmidt David E. , Truedsson Åsa , Strålfors Annelie , Hojbjerg Johanne Andersen , Soutari Nida , Holmström Margareta , Ranta Susanna , Letelier Anna , Bowyer Annette , Ljung Rolf , Antovic Jovan , Bruzelius Maria 
Published: July 2023

Background Discrepancy in factor IX activity (FIX:C) between one-stage assay (OSA) and chromogenic substrate assay (CSA) in patients with hemophilia B (PwHB) introduces challenges for clinical management.
Aim To study the differences in FIX:C using OSA and CSA in moderate and mild hemophilia B (HB), their impact on classification of severity, and correlation with genotype.
Methods Single-center study including 21 genotyped and clinically characterized PwHB. FIX:C by OSA was measured using ActinFSL (Siemens) and CSA by Biophen (Hyphen). In addition, in vitro experiments with wild-type FIX were performed. Reproducibility of CSA was assessed between three European coagulation laboratories.
Results FIX:C by CSA was consistently lower than by OSA, with 10/17 PwHB having a more severe hemophilia type by CSA. OSA displayed a more accurate description of the clinical bleeding severity, compared with CSA. A twofold difference between OSA:CSA FIX:C was present in 12/17 PwHB; all patients had genetic missense variants in the FIX serine protease domain. Discrepancy was also observed with diluted normal plasma, most significant for values below 0.10 IU/mL. Assessment of samples with low FIX:C showed excellent reproducibility of the CSA results between the laboratories.
Conclusion FIX:C was consistently higher by OSA compared with the CSA. Assessing FIX:C by CSA alone would have led to diagnosis of a more severe hemophilia type in a significant proportion of patients. Our study suggests using both OSA and CSA FIX:C together with genotyping to classify HB severity and provide essential information for clinical management.
When Aryne Chemistry Meets Organosulfur Compounds
Authors: Tan Jiajing , Feng Xiaoying , Fan Rong , Zhuang Zhe , Guo Yifeng 
Published: July 2023

AbstractOrganosulfur compounds are ubiquitous in agrochemicals, active pharmaceutical ingredients, natural products, catalysts, and functional materials. The efficient assembly of sulfur-containing skeletons through S(IV) intermediates has emerged as an actively explored direction in organic synthesis. In the past 7 years, our research group has been devoted to developing aryne-induced, sulfonium-zwitterion-based synthetic methodologies. In this account, we systematically overview our recent efforts on this topic, including sigmatropic rearrangements, ring opening of cyclic sulfides, and selective S-arylation of thio-oxindoles. These distinct protocols feature mild conditions and avoid the use of transition metals, allowing facile access to structurally diverse organosulfur compounds. The working hypothesis for our reaction design and key historical precedents are also critically discussed. Our goal is to achieve selectivity control and diversity-oriented synthesis, further advancing sulfonium zwitterion chemistry in the direction of precision synthesis.1	Introduction2	Sigmatropic Rearrangement3	Ring Opening of Cyclic Sulfides4	Selective Arylation of Thio-oxindoles5	Conclusion
Response Assessment of Treated Hepatocellular Carcinoma
Authors: Choudhari Amitkumar J. , Kulkarni Suyash , Shetty Nitin Sudhakar , Gala Kunal , Chandra Daksh , Baheti Akshay D. 
Published: July 2023

AbstractHepatocellular carcinoma (HCC) is a leading cause of morbidity and mortality worldwide, including in India. The incidence of HCC has been rising due to lifestyle diseases such as obesity, diabetes, non-alcoholic fatty liver disease (NAFLD), and alcoholic liver disease (ALD), as well as viral hepatitis infections. Various locoregional therapies (LRTs) are used to treat HCC, including thermal ablation, transarterial therapies, stereotactic body radiotherapy (SBRT), and transarterial radioembolization (TARE). Traditional response evaluation criteria like WHO and RECIST, which rely on size-based measurements, may not accurately assess treatment response to LRTs. To address this limitation, modified response evaluation criteria for solid tumors (mRECIST) and the LI-RADS treatment response algorithm (LR-TRA) have been developed. mRECIST assesses patient-level response, while LR-TRA provides lesion-level response assessment specifically for HCC treated with LRTs. This article discusses the imaging protocols for diagnosing HCC and the imaging appearances of treated lesions after different LRTs. It explains the criteria for categorizing treatment response, such as LR-TR viable, LR-TR non-viable, and LR-TR equivocal. It also highlights the challenges and future directions in response assessment, including the incorporation of ancillary findings, the assessment of patients receiving a combination of locoregional and systemic therapies, and the potential use of biomarkers like serum AFP, AFP-L3, and PIVKA-II. In conclusion, locoregional therapies have expanded the treatment options for HCC, and accurate response assessment is crucial for optimizing patient management. mRECIST and LR-TRA provide valuable tools for evaluating treatment response, and future updates are expected to address specific challenges and incorporate newer approaches like iRECIST and quantitative imaging assessment. Additionally, the use of biomarkers may complement imaging-based response assessment in the future.