bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analisis Pengaruh Literasi Keuangan, Sosialisasi Keuangan Keluarga, dan Uang Saku Terhadap Minat Menabung pada Siswa Usia Remaja Kota Yogyakarta
Authors: Iradianty Aldilla , Azizah Pandan Zahwa 
Published: July 2023

Usia menjadi tumpuan bagaimana perilaku seseorang dalam menabung. Memahami perilaku menabung sedari usia remaja dapat menjadi alat investasi keuangan masa depan yang turut meningkatkan pertumbuhan ekonomi. Penelitian bertujuan untuk menganalisis apakah terdapat dampak dari unsur-unsur eksternal yang mempengaruhi perilaku finansial remaja terhadap kebiasaan menabung. Dalam menjawab pertanyaan ini, peneliti melakukan survey yang relevan terhadap variabel yang diujikan. Survey dilakukan agar dapat menggambarkan situasi sebenarnya. Hasil perhitungan bobot masing-masing pertanyaan pada survey di akumulasi dan di ukur menggunakkan metode SEM (Structural Equation Model). Hasil penelitian mengungkapkan bahwa terdapat hubungan implisit antara masing-masing unsur yaitu literasi keuangan, sosialisasi keuangan keluarga, dan uang saku terhadap minat menabung. Dewasa ini para remaja sudah melakukan pencatatan keuangan. Peran orang tua masih dibutuhkan untuk turut serta mengontrol dan membimbing perilaku keuangan agar tercipta pentingnya minat menabung. Melihat dari segi praktiknya, penelitian ini menekankan perlunya menggalakkan edukasi keuangan secara aplikatif yang sesuai dengan kehidupan nyata.
Isolation and identification of protease-producing Bacillus amyloliquefaciens LX-6 and its application in the solid fermentation of soybean meal
Authors: Huang Xinyi , Li Huijie , Han Tao , Wang Jiteng , Ma Zheng , Yu Xiaoping 
Published: July 2023

Soybean meal (SM) is considered an ideal substitute for fish meal; however, its application is mainly limited because of its antigen proteins, glycinin and β-conglycinin. To improve the value of SM in the aquaculture industry, we employed an aerobic bacterial strain (LX-6) with protease activity of 1,390.6 ± 12.5 U/mL. This strain was isolated from soil samples and identified as Bacillus amyloliquefaciens based on morphological and physiological biochemical characteristics and 16S rDNA gene sequence analyses. Subsequently, we quantified the extent of glycinin and β-conglycinin degradation and the total protein and water-soluble protein content after SM fermentation with B. amyloliquefaciens LX-6. At 24 h of fermentation, the macromolecular antigen proteins of SM were almost completely degraded; the maximum degradation rates of glycinin and β-conglycinin reached 77.9% and 57.1%, respectively. Accordingly, not only did the concentration of water-soluble proteins increase from 5.74% to 44.45% after 48 h of fermentation but so did the concentrations of total protein and amino acids compared to those of unfermented SM. Field emission scanning electron microscopy revealed that the LX-6 strain gradually disrupted the surface structure of SM during the fermentation process. In addition, B. amyloliquefaciens LX-6 exhibited broad-spectrum antagonistic activity and a wide pH tolerance, suggesting its application in SM fermentation for fish meal replacement.
Ex vivo infection model for Francisella using human lung tissue
Authors: Köppen Kristin , Fatykhova Diana , Holland Gudrun , Rauch Jessica , Tappe Dennis , Graff Mareike , Rydzewski Kerstin , Hocke Andreas C. , Hippenstiel Stefan , Heuner Klaus 
Published: July 2023

IntroductionTularemia is mainly caused by Francisella tularensis (Ft) subsp. tularensis (Ftt) and Ft subsp. holarctica (Ftt) in humans and in more than 200 animal species including rabbits and hares. Human clinical manifestations depend on the route of infection and range from flu-like symptoms to severe pneumonia with a mortality rate up to 60% without treatment. So far, only 2D cell culture and animal models are used to study Francisella virulence, but the gained results are transferable to human infections only to a certain extent.MethodIn this study, we firstly established an ex vivo human lung tissue infection model using different Francisella strains: Ftt Life Vaccine Strain (LVS), Ftt LVS ΔiglC, Ftt human clinical isolate A-660 and a German environmental Francisella species strain W12-1067 (F-W12). Human lung tissue was used to determine the colony forming units and to detect infected cell types by using spectral immunofluorescence and electron microscopy. Chemokine and cytokine levels were measured in culture supernatants.ResultsOnly LVS and A-660 were able to grow within the human lung explants, whereas LVS ΔiglC and F-W12 did not replicate. Using human lung tissue, we observed a greater increase of bacterial load per explant for patient isolate A-660 compared to LVS, whereas a similar replication of both strains was observed in cell culture models with human macrophages. Alveolar macrophages were mainly infected in human lung tissue, but Ftt was also sporadically detected within white blood cells. Although Ftt replicated within lung tissue, an overall low induction of pro-inflammatory cytokines and chemokines was observed. A-660-infected lung explants secreted slightly less of IL-1β, MCP-1, IP-10 and IL-6 compared to Ftt LVS-infected explants, suggesting a more repressed immune response for patient isolate A-660. When LVS and A-660 were used for simultaneous co-infections, only the ex vivo model reflected the less virulent phenotype of LVS, as it was outcompeted by A-660.ConclusionWe successfully implemented an ex vivo infection model using human lung tissue for Francisella. The model delivers considerable advantages and is able to discriminate virulent Francisella from less- or non-virulent strains and can be used to investigate the role of specific virulence factors.
An objective approach to select surrogate species for connectivity conservation
Authors: Dutta Trishna , De Barba Marta , Selva Nuria , Fedorca Ancuta Cotovelea , Maiorano Luigi , Thuiller Wilfried , Zedrosser Andreas , Signer Johannes , Pflüger Femke , Frank Shane , Lucas Pablo M. , Balkenhol Niko 
Published: July 2023

IntroductionConnected landscapes can increase the effectiveness of protected areas by facilitating individual movement and gene flow between populations, thereby increasing the persistence of species even in fragmented habitats. Connectivity planning is often based on modeling connectivity for a limited number of species, i.e., “connectivity umbrellas”, which serve as surrogates for co-occurring species. Connectivity umbrellas are usually selected a priori, based on a few life history traits and often without evaluating other species.MethodsWe developed a quantitative method to identify connectivity umbrellas at multiple scales. We demonstrate the approach on the terrestrial large mammal community (24 species) in continental Europe at two scales: 13 geographic biomes and 36 ecoregions, and evaluate the interaction of landscape characteristics on the selection of connectivity umbrellas.ResultsWe show that the number, identity, and attributes of connectivity umbrellas are sensitive to spatial scale and human influence on the landscape. Multiple species were selected as connectivity umbrellas in 92% of the geographic biomes (average of 4.15 species) and 83% of the ecoregions (average of 3.16 species). None of the 24 species evaluated is by itself an effective connectivity umbrella across its entire range. We identified significant interactions between species and landscape attributes. Species selected as connectivity umbrellas in regions with low human influence have higher mean body mass, larger home ranges, longer dispersal distances, smaller geographic ranges, occur at lower population densities, and are of higher conservation concern than connectivity umbrellas in more human-influenced regions. More species are required to meet connectivity targets in regions with high human influence (average of three species) in comparison to regions with low human influence (average of 1.67 species).DiscussionWe conclude that multiple species selected in relation to landscape scale and characteristics are essential to meet connectivity goals. Our approach enhances objectivity in selecting which and how many species are required for connectivity conservation and fosters well-informed decisions, that in turn benefit entire communities and ecosystems.
Endogenous erythropoietin has immunoregulatory functions that limit the expression of autoimmune kidney disease in mice
Authors: Bin Sofia , Cantarelli Chiara , Horwitz Julian K. , Gentile Micaela , Podestà Manuel Alfredo , La Manna Gaetano , Heeger Peter S. , Cravedi Paolo 
Published: July 2023

BackgroundAdministration of recombinant erythropoietin (EPO), a kidney-produced hormone with erythropoietic functions, has been shown to have multiple immunoregulatory effects in mice and humans, but whether physiological levels of EPO regulate immune function in vivo has not been previously evaluated.MethodsWe generated mice in which we could downregulate EPO production using a doxycycline (DOX)-inducible, EPO-specific silencing RNA (shEPOrtTAPOS), and we crossed them with B6.MRL-Faslpr/J mice that develop spontaneous lupus. We treated these B6.MRL/lpr shEPOrtTAPOS with DOX and serially measured anti-dsDNA antibodies, analyzed immune subsets by flow cytometry, and evaluated clinical signs of disease activity over 6 months of age in B6.MRL/lpr shEPOrtTAPOS and in congenic shEPOrtTANEG controls.ResultsIn B6.MRL/lpr mice, Epo downregulation augmented anti-dsDNA autoantibody levels and increased disease severity and percentages of germinal center B cells compared with controls. It also increased intracellular levels of IL-6 and MCP-1 in macrophages.DiscussionOur data in a murine model of lupus document that endogenous EPO reduces T- and B-cell activation and autoantibody production, supporting the conclusion that EPO physiologically acts as a counterregulatory mechanism to control immune homeostasis.
Anxiolytic effects of Chrysanthemum morifolium Ramat Carbonisata-based carbon dots in mCPP-induced anxiety-like behavior in mice: a nature-inspired approach
Authors: Cui Luming , Zhang Qian , Zhang Yifan , Li Tingjie , Li Menghan , Yuan Jinye , Wu Zhiyi , Zhang Yue , Kong Hui , Qu Huihua , Zhao Yan 
Published: July 2023

Introduction: Anxiety disorders have emerged as a predominant health concern, yet existing pharmacological treatments for anxiety still present various challenges. Chrysanthemum morifolium Ramat Carbonisata (CMRC) has been utilized in China for approximately 400 years as a therapeutic intervention for anxiety disorders. In this study, a novel type of carbon dots derived from the decoction of Chrysanthemum morifolium Ramat Carbonisata (CMRC-CDs) was identified and isolated, and their morphological structure and functional groups were characterized. Furthermore, the effects of CMRC-CDs on m-chlorophenylpiperazine (mCPP)-induced anxiety-like behaviour in mice were examined and quantified. In order to investigate the potential mechanisms of their anxiolytic effects, concentrations of hypothalamic-pituitary-adrenal (HPA) axis hormones, amino acid neurotransmitters, and monoamine neurotransmitters were measured.Methods: In this study, we synthesized CMRC-CDs and evaluated their potential anti-anxiety effects in a controlled experiment involving 48 male ICR mice. The mice were randomly divided into six groups, treated with CMRC-CDs at different doses for 14 days, and subjected to Open-Field (OF) and Elevated Plus Maze (EPM) tests. Post-behavioral evaluations, blood samples and brain tissues were collected for neurotransmitter and Hypothalamic-Pituitary-Adrenal (HPA) axis hormone quantification via ELISA. Additionally, cytotoxicity of CMRC-CDs was assessed using a Cell Counting Kit-8 (CCK-8) assay on RAW 264.7 cells.Results and Discussion: CMRC-CDs were spherical and homogeneously dispersed, with diameters ranging from 1.4 to 4.0 nm and an abundance of chemical groups on their surface. In the open-field (OF) test, mice pre-treated with CMRC-CDs demonstrated an increased proportion of time spent in the central area and a higher frequency of entries into the central area. In the elevated plus maze (EPM) test, mice pre-treated with CMRC-CDs exhibited a greater number of entries into the open arm and an extended duration spent in the open arm. CMRC-CDs were observed to decrease serum concentrations of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and corticosterone (CORT). Furthermore, CMRC-CDs were found to increase γ-aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) levels, while concurrently reducing glutamic acid (Glu) concentrations in brain tissue. CMRC-CDs demonstrated anxiolytic effects, which may be attributed to their modulation of hormones and neurotransmitters. This finding suggests the potential therapeutic value of CMRC-CDs in the clinical treatment of anxiety disorders.
“Bitcoin now”: temporal discounting in Bitcoin holders
Authors: El Haj Mohamad , Moustafa Ahmed A. 
Published: July 2023

IntroductionCryptocurrency investment and trading are rapidly growing activities due to the development of applications and platforms that offer fast, continuous, and easy entry into the cryptocurrency world. To understand decision making in cryptocurrency holders, we assessed temporal discounting, that is, whether Bitcoin holders disregard rewards if they are distant in time and overvalue rewards if they are more immediate. Further, we compared performance between short-term investors (i.e., day-traders) vs. long-term investors.MethodsUsing an online survey, we invited 144 Bitcoin holders to answer temporal discounting questionnaires dealing with money (“Which do you prefer, that you get right now 20 USD in cash or 100 USD in a month?”) and Bitcoin (“Which do you prefer, that you get right now 0.1 or 1 Bitcoin in a month?”).ResultsAnalysis demonstrated no significant differences between temporal discounting for money and Bitcoin. However, and critically, higher temporal discounting for both money and Bitcoin was observed in short-term investors compared with long-term investors. In a similar vein, significant positive correlations were observed between day trading and temporal discounting for both money and Bitcoin.DiscussionThese findings demonstrate how Bitcoin holders with short-term time horizons tend to prioritize immediate rewards over larger but delayed rewards. Future research can assess the neural basis of temporal discounting for cryptocurrencies.
Efficacy, safety and tolerability of very low-calorie ketogenic diet in obese women with fibromyalgia: a pilot interventional study
Authors: Ciaffi Jacopo , Lisi Lucia , Mari Anna , Mancarella Luana , Brusi Veronica , Pignatti Federica , Ricci Susanna , Vitali Giorgia , Stefanelli Nicola , Assirelli Elisa , Neri Simona , Naldi Susanna , Faldini Cesare , Ursini Francesco 
Published: July 2023

IntroductionObesity can worsen fibromyalgia (FM) and very low-calorie ketogenic diet (VLCKD) is a potential therapeutic option for diseases that share clinical and pathophysiological features with FM. In this pilot interventional study, we investigated the effects of VLCKD in obese women with FM.MethodsFemale patients with FM and a body mass index (BMI) ≥ 30  kg/m2 were eligible for VLCKD. The ketogenic phase (T0 to T8) was followed by progressive reintroduction of carbohydrates (T8 to T20). Changes in BMI, Fibromyalgia Impact Questionnaire (FIQ), Hospital Anxiety and Depression Scale (HADS), EuroQol 5D (EQ-5D) and 36-item Short Form Health Survey (SF-36) were evaluated. A change of 14% in FIQ was considered clinically relevant. The longitudinal association between BMI and patient-reported outcomes (PROs) was assessed using generalized estimating equations.ResultsTwenty women were enrolled. Two discontinued the intervention. The mean age of the 18 patients who reached T20 was 51.3  years and mean BMI was 37.2  kg/m2. All patients lost weight during the first period of VLCKD and this achievement was maintained at T20. Mean BMI decreased from 37.2  kg/m2 at T0 to 34.8  kg/m2 at T4, 33.5  kg/m2 at T8 and 32.1  kg/m2 at T20 (p &lt; 0.001). A significant reduction of mean FIQ from 61.7 at T0 to 37.0 at T4 and to 38.7 at T8 (p &lt; 0.001) was observed and it was maintained at T20 with a mean FIQ of 39.1 (p = 0.002). Similar results were obtained for HADS, EQ-5D and SF-36. Analysing each participant, the reduction of FIQ was clinically meaningful in 16 patients (89%) at T4, in 13 (72%) at T8 and in 14 (78%) at T20. No significant association was observed between change in BMI and improvement of the PROs over time. Adverse effects were mild and transient. No major safety concerns emerged.ConclusionThese are the first data on the efficacy of VLCKD in FM. All patients achieved improvement in different domains of the disease, which was maintained also after carbohydrate reintroduction. Our results suggest that ketosis might exert beneficial effects in FM beyond the rapid weight loss.Clinical trial registrationThis trial is registered on ClinicalTrials.gov, number NCT05848544.
Angiopoietin-2 expression and its relationship with lymphangiogenesis and clinicopathological characteristics in cutaneous malignant melanoma
Authors: Zheng Wei , Ju Wei , Yang Xi-Hu , Yan Zhi-Xin 
Published: July 2023

ObjectiveThe aim of this study was to investigate angiopoietin-2 (Ang-2/ANGPT2) expression and its relationship with lymphangiogenesis and clinicopathological characteristics in cutaneous malignant melanoma (CMM).MethodsGene expression differences between metastatic melanoma and melanoma in situ in 472 patients from the TCGA database were analyzed. The target gene Ang-2 was screened. A clinical study was conducted to analyze the correlation between Ang-2 expression in CMM and tumor-associated lymphangiogenesis. A total of 42 patients with primary CMM who underwent extended tumor resection procedures at the Affiliated Hospital of Jiangsu University were included in this study. Clinical data (gender, age, lymph node metastasis, Breslow thickness, and clinical stage) were collected. The expression levels of both Ang-2 and lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) proteins were detected by immunohistochemistry (IHC). Lymphatic vascular density (LVD) was counted by using LYVE-1 to label lymphatic endothelial cells (LECs) in peritumoral and intratumoral areas per high-magnification field of view. Statistical analysis was performed using the Pearson correlation test and Student’s t-test.ResultsUsing the TCGA database, it was found that the gene expression level of Ang-2 in 368 cases of metastatic melanoma was significantly higher than that in 104 cases of melanoma in situ. Correlation analysis showed a significant relationship between Ang-2 and LYVE-1, and vascular endothelial growth factor receptor 3(VEGFR3) expression, respectively, in CMM. Moreover, the optimal cutoff value of survival analysis showed that high Ang-2 expression in CMM had a worse prognosis, based on data from the TCGA database. Our research showed that Ang-2 was more highly expressed in the group of patients with lymph node metastasis and in the group of stage 3C-4 patients than in the group of patients with no lymph node metastasis and in the group of stage 0-3B patients. Our research also showed that LVD in the group of patients with lymph node metastasis and in the group of stage 3C-4 patients was significantly higher than that in the group of no lymph node metastasis and in the group of stage 0-3B patients, respectively. Breslow thickness also correlated with Ang-2 expression and LVD. Ang-2 expression was not related to sex or age. Ang-2 expression was obviously correlated with LVD.ConclusionAn evaluation of Ang-2 expression and LVD can be used to predict the risk of tumor lymphatic metastasis and determine the prognosis of CMM. These results may also provide a new clinical treatment strategy for CMM.
Delayed chylopericardium after radical surgery for esophageal cancer: a case report
Authors: Yang Pengjie , Han Rui , Zhu Benben , Wu Yu , Han Bater 
Published: July 2023

BackgroundPostoperative chylpericardium is a rare clinical disease that often manifests as chest tightness, shortness of breathdyspnea, and other symptoms of pericardial tamponade. The etiological spectrum of chylopericardium is complex, but the disease is mainly idiopathic. Chylopericardium caused by thoracic surgery is rarely reported, both at home and abroad.Case summaryWe report a case of a 65-year-old male patient who developed chylopericardium after thoracoabdominal combined incision and partial esophagogastric anastomosis plus lymph node dissection for 1 month. After pericardiocentesis and drainage, low-fat enteral nutrition, and parenteral nutrition, the patient was cured. Based on this case, this article reviews the literature on the diagnosis and treatment of chylopericardium after thoracic surgery.ConclusionIn conclusion, thoracic surgery (excluding cardiac surgery) can cause delayed chylopericardium. This condition is rarely reported in China, and only a few cases have been reported abroad. Thus, the diagnosis is likely to be missed or misdiagnosed. Early diagnosis and treatment are important to reduce patient discomfort as much as possible.
Treatment of immunoglobulin-resistant kawasaki disease: a Bayesian network meta-analysis of different regimens
Authors: Pan Yan , Fan Qihong , Hu Luoyi 
Published: July 2023

BackgroundThis study aimed to gather evidence from clinical trials on the efficacy and safety of the available treatments for intravenous immunoglobulin (IVIG)-resistant Kawasaki disease (KD) in children.MethodsThis work adopted the Newcastle–Ottawa scale to analyse the quality of the enrolled articles. A network meta-analysis was performed using clinical trials that compared drugs used to treat IVIG-resistant KD. Aggregate Data Drug Information System software v.1.16.5 was employed to analyse whether infliximab, second IVIG infusions, and intravenous pulse methylprednisolone (IVMP) were safe and effective.ResultsTen studies, involving 704 patients with IVIG-resistant KD, were identified and analysed. Overall, infliximab exhibited remarkable antipyretic activity compared with the second IVIG infusions (2.46, 1.00–6.94). According to the drug rank, infliximab was the best option against IVIG-resistant KD. Regarding adverse effects, the infliximab group was more prone to hepatomegaly. A second IVIG infusion was more likely to result in haemolytic anaemia. IVMP treatment was more susceptible to bradycardia, hyperglycaemia, hypertension, and hypothermia. In addition, infliximab, IVMP, and the second IVIG infusions showed no significant differences in the risk of developing a coronary artery aneurysm (CAA).ConclusionInfliximab was the best option against IVIG-resistant KD, and IVMP, infliximab, and second IVIG infusions have not significant differences of prevent CAA in patients with IVIG-resistant KD.Systematic Review RegistrationIdentifier: https://osf.io/3894y.