bluuebunny/crossref_metadata_2025_split huggingface dataset:
12R-HETE acts as an endogenous ligand of Nur77 in intestine and regulates ILC3s plasticity
Authors: Huang Ningning , Ye Ling , Li Hao , Wei Hongkui , Peng Jian 
Published: June 2023

ABSTRACTGroup 3 innate lymphoid cells (ILC3s), a heterogeneous population, are tissue-resident myeloid cells and have an essential role in bacterial infection. Although the plasticity of NKp46-CCR6-double-negative (DN) ILC3s toward the NKp46+ILC3s is an important process in the development of intestinal immunity, the underlying molecular mechanisms responsible for this process remain poorly understood. Nur77 is an orphan receptor which regulates intestinal ILC3s expansion. However, the impact of Nur77 on the plasticity of intestinal ILC3s remains unclear. Here, we generatedNur77null mice and investigated ILC3s expansion. The deficiency ofNur77inhibited the mouse small intestinal ILC3s expansion and conversion of NKp46-ILC3s to NKp46+ILC3s. We identified that 12R-HETE derived from arachidonic acid (ARA) in mouse intestine is an endogenous ligand of Nur77 and activates its transcriptional activity. The treatment with 12R-HETE promoted the differentiation of NKp46-ILC3s into NKp46+ILC3s by enhancing the T-bet expression, thereby increased IFN-γ production from NKp46+ILC3s, and reduced the susceptibility to bacterial infection in WT, but not Nur77-/-, suckling mice. An integrated analysis of ATAC-seq and Smart RNA-seq showed thatRflnb,Impdh1,Map1s, andGtpbp3might be downstream targeted genes of Nur77 in response to 12R-HETE and mediate the regulation of ILC3s plasticity. In the presence of mycophenolic acid, an inhibitor of IMPDH, 12R-HETE no longer regulated the percentages of RORγt+ILC3s and NKp46+ILC3s. We conclude that 12R-HETE acts as an endogenous ligand of Nur77, and regulates the ILC3s expansion and plasticity, and in turn, gut homeostasis and pathogen defense.
Imposter Phenomenon in licensed physical therapists: prevalence, predictors, and impact
Authors: Anderson Alexandra R. , LaPenna Jamie , Willis Dustin , Chang Alison H. 
Published: June 2023

ABSTRACTObjectiveDetermine the prevalence and predictors of Imposter Phenomenon (IP) among licensed physical therapists (PTs) in the United States.MethodsThis cross-sectional observational study utilized an online survey to assess the levels of IP, emotional exhaustion, and job satisfaction among licensed PTs in the United States and collect their professional and demographic information. Participants were recruited from various physical therapy specialty groups representing a range of professional memberships and certifications. A multivariable logistic regression model examined factors associated with IP presence.ResultsThe study sample consisted of 514 PTs [women (66.1%), ≤ 40 years of age (73.2%), White (77.0%)]. The mean IP score was 60.3 (SD: 15.1, range: 19 to 95). 55 respondents (10.7%) had low IP, 196 (38.1%) moderate IP, 215 (41.8%) frequent IP, and 48 (9.3%) intense IP. The prevalence of IP, defined as frequent or intense IP, was 51.2% in our study sample. Having Manager/supervisor experience (OR=0.55, 95% CI=0.34-0.90) was associated with a reduced odds of IP presence. Holding a bachelor’s or master’s degree (vs. Doctor of Physical Therapy (DPT) degree; OR=2.31, 95% CI=1.07-5.00), a history of or current mental health diagnosis (OR=2.77, 95% CI=1.69-4.54), and emotional exhaustion (moderate vs. low: OR=5.37, 95% CI=2.11-13.69; high vs. low: OR=14.13, 95%CI=5.56-35.89) were each associated with an increased odds of IP presence.ConclusionsIP is highly prevalent among licensed PTs, with more than half reporting frequent or intense IP. Seasoned clinicians with managerial roles seemed to be less susceptible to IP, whereas those with mental health diagnoses, emotional exhaustion, and those without a DPT degree may be more likely to experience IP.Impact StatementGiven the high prevalence of IP and its potential negative impact on burnout and career advancement, it is crucial to increase IP awareness and provide education on strategies and resources to manage it for licensed PTs.
Two-color coincidence single-molecule pull-down for the specific detection of disease-associated protein aggregates
Authors: Saleeb Rebecca S. , Leighton Craig , Lee Ji-Eun , O’Shaughnessy Judi , Jeacock Kiani , Chappard Alexandre , Cumberland Robyn , Ball Sarah R. , Sunde Margaret , Clarke David J. , Piché Kristin , McPhail Jacob A. , Louwrier Ariel , Angers Rachel , Gandhi Sonia , Downey Patrick , Kunath Tilo , Horrocks Mathew H. 
Published: June 2023

AbstractThe misfolding and aggregation of protein is a characteristic of many neurodegenerative disorders, including Alzheimer’s and Parkinson’s disease. The wide range of sizes and structures of oligomers and fibrils generated have previously been studied using single-molecule and super-resolution microscopy. These methods, however, tend to rely on the use of either directly labeled protein, or on the addition of non-specific amyloid stains, such as thioflavin-T. This has prevented the characterization of protein aggregate composition in complex biological samples. Here, we have developed a single-molecule two-color aggregate pull-down (STAPull) assay to overcome this challenge by probing immobilized proteins using orthogonally labeled antibodies targeting the same epitope. By looking at colocalized signals, we can eliminate monomeric protein, and specifically quantify aggregated proteins. Using the aggregation-prone alpha-synuclein protein as a model, we demonstrate that this approach can specifically detect aggregates with a limit of detection of 5 pM. Furthermore, we show that STAPull can be used in a range of samples, including in human biofluids. STAPull is generally applicable to protein aggregates from a variety of disorders, and will aid in the identification of biomarkers that are crucial in the effort to diagnose these diseases.
Genetic Diversity Modulates The Physical And Transcriptomic Response Of Skeletal Muscle To Simulated Microgravity
Authors: Zeineddine Yasmina , Friedman Michael A. , Buettmann Evan G. , Abraham Lovell B. , Hoppock Gabriel A. , Donahue Henry J. 
Published: June 2023

AbstractDevelopments in long-term space exploration necessitate advancements in countermeasures against microgravity-induced skeletal muscle loss. Astronaut data shows considerable variation in muscle loss in response to microgravity. Previous experiments suggest that genetic background influences the skeletal muscle response to unloading, but no in-depth analysis of genetic expression was performed. Here, we placed eight inbred founder strains of the diversity outbred mice (129S1/SvImJ, A/J, C57BL/6J, CAST/EiJ, NOD/ShiLtJ, NZO/HILtJ, PWK/PhJ, and WSB/EiJ) in simulated microgravity (SM) via hindlimb unloading for three weeks. Body weight, muscle morphology, muscle strength, protein synthesis marker expression, and RNA expression were collected. A/J and CAST/EiJ mice were most susceptible to SM-induced muscle loss, whereas NOD/ShiLtJ mice were the most protected. In response to SM, A/J and CAST/EiJ mice experienced reductions in body weight, muscle mass, muscle volume, and muscle cross-sectional area. A/J mice had the highest number of differentially expressed genes (68) and associated gene ontologies (328). Downregulation of immunological gene ontologies and genes encoding anabolic immune factors suggest that immune dysregulation contributes to the response of A/J mice to SM. Several muscle properties showed significant interactions between SM and mouse strain and a high degree of heritability. These data imply that genetic background plays a role in the degree of muscle loss in SM and that more individualized programs should be developed for astronauts to protect their skeletal muscles against microgravity on long term missions.
A clinical decision support system for interventional urinary stone management planning
Authors: Bhuphaalan Soorya , Vivekanandhan Sapthagirivasan 
Published: June 2023

AbstractUrinary stone disease is a very common disease of the urinary tract where excessive mineral content in the kidneys form stones and obstruct the urinary tract, due to which affected patients experience severe pain and uneasiness. Identification of the appropriate interventional stone management method is usually done based on the size and location of the stone. Improper planning of the interventional method can lead to multiple revisits and unnecessary radiation exposure and thus an intelligent clinical decision system to precisely provide a treatment plan is required. In this paper, an intelligent system that recommends an appropriate interventional stone management system is proposed. Stone management data of 600 patients containing information about the stone size, location and the treatment provided to them was used to train machine learning models. The training and testing performance of different machine learning models with the dataset has been compared. Results showed that decision trees and support vector machines showed better results in predicting the right treatment planning method while given necessary inputs (stone size and location). This system can be useful in clinical setups in assisting urologists in planning treatment for urinary stone disease.
A rapid, inexpensive, non-lethal method for detecting disseminated neoplasia in a bivalve
Authors: Vandepas Lauren E. , Crim Ryan N. , Gilbertson Emily , Yonemitsu Marisa A. , Unsell Elizabeth , Metzger Michael J. , Lacy-Hulbert Adam , Goetz Frederick W. 
Published: June 2023

ABSTRACTDisseminated neoplasia (DN) is a form of cancer in bivalve molluscs that has been reported in some cases to be a transmissible cancer. Neoplastic cells are highly proliferative, and infection is often lethal. Some commercially valuable bivalve species (mussels, cockles, soft-shell clams, oysters) are affected by outbreaks of disseminated neoplasia, making disease diagnosis and mitigation an important issue in aquaculture and ecological restoration efforts. Here we describe a minimally invasive, non-lethal method for high-throughput screening for disseminated neoplasia in basket cockles (Clinocardium nuttallii). Basket cockles are native to the North American Pacific coast from California to Alaska. There is recent concern from some Coast Salish Tribes regarding an observed long-term decline in cockle populations in Puget Sound, WA. This has led to increased interest in monitoring efforts and research to improve our understanding of the mechanisms of observed basket cockle population dynamics, including assessing prevalence of disease, such as disseminated neoplasia. The rapid, non-lethal hemolymph smear screening method presented here to diagnose DN in adultC. nuttalliican be applied at field sites at low financial cost, and in a validation study of 29 animals the results were identical to that of the gold standard method, tissue histology. Due to the similar morphology of DN in different bivalves, this method can likely be generally applied for use in any bivalve species.
Transcriptome based changes in expression of detoxification genes ofBemisia tabaciunder destruxin A treatment
Authors: Zhang Can , Guo Jianling , Ali Shaukat , Qiu Bao-Li 
Published: June 2023

AbstractBackgroundBemisia tabaciis an important agricultural pest that has been causing significant economic losses to crops across the globe. Destruxins are secondary metabolites of entomopathogenic fungi which can be used as a potential biopesticide againstB. tabaci. However, little is known about the molecular mechanism regulating the defense response ofB. tabacipost destruxin application.ResultsIn this study, we explored the molecular responses ofB. tabaciexposed to destruxin A (DA) using RNA-Seq and differentially expressed gene (DEG) analysis. A total of 1702, 616, and 555 DEGs were identified inB. tabaciafter 4, 8, and 12 h of destruxin A treatment. In addition, 40 putative detoxification-related DEGs, including 29 cytochrome P450s (P450s), 5 glutathione S-transferases (GSTs), and 5 carboxylesterases (CarEs) were also identified. Quantitative real-time PCR analysis indicated that the expression profiles of 19 random DEGs were consistent with the RNA-Seq results.ConclusionThese findings serve as valuable information for a better understanding of the interaction and molecular mechanisms involved in the defense response ofB. tabaciagainst DA.
Optimization and understanding of corrosion inhibitors for cooling water system
Authors: Li Zimin , Zhu Zilong , Wang Jianqiu , Wang Meng , Hou Ting , Li Qinghua , Yu Pei 
Published: June 2023

Purpose
Two corrosion inhibitors for closed cooling water systems, nitrite-based and mixture of nitrite and molybdate corrosion inhibitor, are often compared to each other. This study aims to optimize these two inhibitors in terms of concentration and pH for carbon steel protection, with insights into the double layer structure on surface and its impact on corrosion inhibition.

Design/methodology/approach
Electrochemical analysis including electrochemical impedance spectroscopy and potentiodynamic test are carried out for quick assessment of corrosion inhibition efficiency and optimization, which is confirmed by immersion test and microscopic analysis. The electronic properties of the surface film are analyzed through Mott–Schottky method which provides new insights into the inhibition mechanism and the role of each component in mixture inhibitor.

Findings
Mixture of nitrite and molybdate is shown to present higher inhibition efficiency, owning to the double layer structure. Nitrite alone can form a protective surface film, whereas molybdate leads to an n-type semiconductive film with lower donor density, hence giving rise to a better inhibition effect.

Research limitations/implications
Surface after inhibitor treatment has been carefully characterized to the microscopic scale, implying the effect of micro-structure, chemical composition and electronic properties on the corrosion resistance. Inorganic corrosion inhibitors can be tuned to provide higher efficiency by careful design of surface film structure and composition.

Originality/value
Almost every study on corrosion inhibitor applies such method for quick assessment of corrosion inhibition effect. Mott–Schottky test is one of electrochemical methods that reveals the electronic properties of the surface film. Previous works have studied the surface layer mainly through X-ray photoelectron spectroscopy. This study provides another insight into the surface film treated by nitrite and molybdate through Mott–Schottky analysis, and relates this structure to the corrosion inhibition effect based on multiple analysis including electrochemistry, microscopic characterization, thermodynamics and interface chemistry.
Analysis of the mechanical limitations of the selected high-speed electric motor
Authors: Korkosz Mariusz , Noga Stanisław , Rogalski Tomasz 
Published: June 2023

Purpose
The study aims to show the influence of selected mechanical parameters of the rotor on the maximum speed and parameters of the electric motor.

Design/methodology/approach
A simplified mechanical analysis of the rotor of the electric motor was conducted, determining the safety factor of the motor. An analysis of the impact of key rotor parameters (significant from the mechanical strength perspective) on the electromagnetic parameters and the safety factor of the selected high-speed electric motor was carried out. The influence of changes in the rotor’s geometrical dimensions (centrifugal force) on the electromagnetic parameters of the electric motor was shown.

Findings
The study shows the impact of changes in selected rotor parameters on electromagnetic parameters and the safety factor of a high-speed electric motor (at its required operating point of 45,000 rpm). The dependence of the safety factor as a function of the maximum motor speed was determined for the proposed rotor modifications.

Practical implications
The proposed modifications can be used in larger drive systems. They have practically no impact on increasing the value of the motor’s moment of inertia (they do not degrade the dynamics of the motor’s operation).

Originality/value
It was proposed to use a new design coefficient which is in relation to the motor’s safety coefficient. It has been shown that a minimal modification of the motor rotor allows to increase its maximum speed by several dozen per cent (while maintaining the safety factor). It has also been shown that when operating at maximum speed within the safe range, the change in the geometrical dimensions of the rotor hardly influences the change in the value of the centrifugal force.
Entrepreneurial orientation and competitive aggressiveness: A need for conceptual refinement and contextualization
Authors: Al-Harthi Salem , Bachkirov Alexandre Anatolievich , Al-Riyami Said , Al-Jahwari Misida 
Published: June 2023

PurposeThe purpose of this paper is to evaluate the relevant literature to gain deeper insights into understanding what directions of research are needed with reference to entrepreneurial orientation and competitive aggressiveness in the oil and gas sector of the Gulf Cooperation Council (GCC) region.Design/methodology/approachThis paper is a conceptual evaluation of literature.FindingsThe extant body of research on both entrepreneurial orientation and competitive aggressiveness is based on quantitative studies without previous systematic, exhaustive and comprehensive grounded theory-based theorizing processes rooted in qualitative approaches. This accounts for inconsistencies and controversies of findings reported in the field.Practical implicationsThe grounded theory-based approach advocated by this paper is likely to generate a more precise estimation of the relationship between entrepreneurial orientation, competitive aggressiveness and firm performance. Consequently, an accurate understanding of the interplay between these factors can empower managers to make finely-tuned strategic decisions, achieve sustainable competitive advantage and optimally adapt to dynamic and unforeseen environments.Originality/valueThe paper reports on important limitations of the existing literature indicating that current findings may be an artifact of studying a small number of industries.
How entrepreneurial implementation intention moves toward subsequent actions: affordable loss and environmental uncertainty
Authors: He Liang-Xing , Li Teng 
Published: June 2023

Purpose
The purpose of this paper is to bridge the gap between entrepreneurial implementation intention and subsequent actions, addressing the isotropic issue under uncertain entrepreneurship.

Design/methodology/approach
The authors conducted two rounds surveys, a total of 2,350 individuals are surveyed, and 240 of whom expressed entrepreneurial intention but had yet to start a business comprised the sample.

Findings
This research finds that entrepreneurial implementation intention has a significant positive relationship with subsequent actions, affordable loss mediates the effect of implementation intention on subsequent actions, environmental uncertainty negatively moderates the relationship between affordable loss and subsequent actions, and the indirect effect of entrepreneurial implementation intention on entrepreneurial action can be enhanced at the low level of environmental uncertainty.

Originality/value
This study contributes new insights to the literature on Rubicon model of action phases in entrepreneurship field by using affordable loss and uncertainty. It also contributes to the literature on affordable loss by examining how environmental uncertainty conditions the effect of affordable loss on entrepreneurial action. Additionally, the negatively moderating role of environmental uncertainty offers a new possibility to explain entrepreneurial uncertainty.
Determinants of adoption and usage of the online examination portal (OEP) in Indian universities
Authors: Shirolkar Shweta Devendra , Kadam Rajashri 
Published: June 2023

PurposeThe purpose of this study is to examine the factors influencing the adoption and utilization of the online examination portal (OEP) in Indian universities. This study combines the Unified Theory of Acceptance and Usage of Technology (UTAUT) and Task Technology Fit (TTF) to explain the user's intention to use an OEP at an Indian University.Design/methodology/approachThis study's methodology combines UTAUT as a theoretical framework with TTF. It is conceptualized that TTF has a moderating effect on UTAUT's primary construct. The authors collected the data from 182 graduate students from an Indian university. PLS-SEM is used to identify the causal relationship between the constructs using the structural equation modeling (SEM) technique.FindingsThe findings will demonstrate the applicability of the constructs of performance expectancy, effort expectancy and facilitating conditions in influencing the usage intention and behavior of students towards OEP. The outcome will also demonstrate the moderating effect of TTF on the predictors of intention to use OEP at an Indian University.Research limitations/implicationsThe sample respondents who participated in the study are students at the Indian University in Pune. The selection of samples was based on convenience sampling. Future research should be conducted at a variety of geographic locations, educational levels, undergraduate and graduate schools, as well as multiple universities. To increase the objectivity of sample selection, the probabilistic sampling method can be used to select samples. For future studies, larger sample sizes (more than 182) can be collected in order to increase the generalizability and applicability of the findings to other developing markets.Practical implicationsThe study will aid Indian universities in enhancing the effectiveness of OEP. The study will also demonstrate the users' perception of the portal and their willingness to use it. It will also assist the university administration in finalizing the examination portal's implementation strategy.Originality/valueVery few studies have been published on the factors influencing the acceptance and utilization of specific online examination systems. To address this underrepresentation, the current article investigates the factors and criteria that influence students' intent to use the OEP. The authors conceptualize the model using UTAUT as a theoretical foundation, which is absent from the published literature on the OEP. The study incorporated UTAUT and TTF to determine the impact on OEP usage and intent. In addition, the study contributes by testing the moderating effect of TTF on the UTAUT variables that influence the intention to use OEP.
Digital resilience and firm internationalization: a study of Chinese listed companies
Authors: Li Zhaochen , Xu Zimu , Sukumar Arun 
Published: June 2023

PurposeThe aim of this research is to explore the impact of digital resilience on firms' international performance. Using internal operation efficiency and innovation as channel variables, this paper explores the relationship between digital resilience and international firm performance of Chinese listed firms.Design/methodology/approachThe research design follows a quantitative approach. Using firm-level panel data from 2007 to 2020, this paper tests the hypotheses between digital resilience and international firm performance through internal efficiency and innovation.FindingsThe results note that digital resilience has a positive effect on internationalization while operation efficiency is a channel through which digital resilience promotes internationalization. Digital resilience also facilitates innovation by improving research and development (R&D) efficiency and matching innovation collaborators.Originality/valueThis paper is one of the first to explore digital resilience in the context of internalization and international firm performance. This paper extends the notion of resource-based view (RBV) to examine the relationship between digital resilience, internal efficiency and innovation on international firm performance.