bluuebunny/crossref_metadata_2025_split huggingface dataset:
Micro-RNA (miRNA) epigenetic profiling of plasma small extracellular vesicles (sEV) – sEV miRNA as diagnostic biomarker of fertility
Authors: Abeysinghe Pevindu , Turner Natalie , Flay Holly , Meier Susanne , Cuttle Leila , Mitchell Murray 
Published: August 2023

Abstract
Fertility is determined to a significant extent by its underlying genetics and success of pregnancy is considered as a tool to define fertility. A substantial knowledge gap exists however, regarding epigenetic abnormalities resulting in infertility. The accuracy of information concerning fertility is critical to the success of an infertility treatment plan. Here, the authors explore the use and the value of blood plasma small extracellular vesicle (sEV) derived micro-RNA (miRNA) as biomarkers of fertility. Next-generation miRNA sequencing identified 14 differentially expressed (DE) miRNAs expressed with a substantial confidence between low fertile (LF) sEV and high fertile (HF) sEV (FDR < 0.05 and -logFC > 2), isolated from plasma of dairy cows (n = 10 per each HF and LF group). Interestingly, the majority of DE miRNAs were uniquely packaged into sEV and not found in circulating plasma. Validation using qRT-PCR miRNA assays indicated similar expression patterns of miR-17-5p, miR-2285dd, miR-2335, miR-12054 and miR-2285aw, and confirmed that miR-181b-5p was significantly upregulated in LF sEV (P value = 0.0093, Fold change = 2.665). The results from this study suggest that circulating sEV miRNA reflect the overall fertility status including the physiological status of the endometrium. Moreover, miR-181b-5p was validated as a prognostic sEV miRNA biomarker of fertility.
Study on the floor failure ‘two-zone’ characteristics and the ‘dual key layer’ water control theory in floor grouted mining working face
Authors: Miao Wei , Xu Yanchun , Guo Dongyu , Xing Chaorui , Zhang Bosong , Li Lei , Li Jun 
Published: August 2023

Abstract
In Jiaozuo mining area of North China coal field, there are multiple strong water-richness aquifers close to the coal seam. In order to prevent floor water inrush accident, it is need to be reinforced by floor grouting before mining. According to the changes of the mechanics and hydrogeological properties of the floor key rock strata after floor grouting, the coal seam floor is divided into ‘two zones’ of ‘water conduction fracture zone’ and ‘mechanical strength damage zone’. The composite water control model of ‘floor structure stability water control key layer’ and ‘floor damage infiltration water control key layer’ is established after grouting reinforcement of upper hard rock aquifer. Microseismic(MS) technology is used to analyse the characteristics of ‘two zones’ and the water control capability of ‘dual key layers’ in fault regions and no-fault regions respectively of experimental working face. It is revealed that the L8 limestone plays the role of mechanical barrier key layer after grouting. The change of elastic modulus of rock mass before and after grouting is measured by borehole ultrasonic method, which result shows that the elastic modulus of different rock mass increases by 40–852% respectively after grouting. By using the formula method, FLAC3D numerical simulation and field measurement, it is found that the failure depth of the floor after grouting is reduced by 51% compared with that without grouting. Seven influencing factors for floor water control ‘dual key layer’ are proposed, which are quantified by AHP (Analytic Hierarchy Process) method. A scheme of ‘dual key layer’ water control capability evaluation is formed by AHP method and influencing factors evaluation method. It is revealed that fault activation and fault with water-filling are the main factors that affect the failure of water control capability of ‘dual key layer’ and floor water inrush. The results of the research are consistent with the water inrush of the experimental working face, which will contribute to the early warning, prevention and evaluation of floor water inrush in mines with the same hydrogeological conditions.
Evaluation of the impacts of occupational exposure to PM and PAH on cardiovascular problems in wastewater treatment plant workers
Authors: Saad-Hussein Amal , Beshir Safia , Shaheen Weam , Saleh Inas A. , Hafez Salwa , Mohammed Atef M.F. 
Published: August 2023

Abstract

Background
Workers in wastewater treatment plant (WWTPs) are at high risk of developing various infections and cardiovascular diseases (CVD).
Aims
of the study were estimation of the concentrations of 16 EPA priority polycyclic aromatic hydrocarbons (PAHs) and particulate matters (PM) with different sizes in air of WWTP, and prediction of CVDs to evaluate the potential risk of developing CVDs in the WWTP workers due to their occupational exposures, in addition to identification of the CVDs` risky work tasks. Methodology: PM concentrations, and wind rose plots for meteorological data, were monitored for 24 months. Estimation of the individual PAHs in suspended particulate matter samples and identification of sources were done. Cross-sectional study was done on 142 male workers from WWTP. All participants were subjected to occupational and medical questionnaire, electrocardiogram (ECG), and measurement of blood pressure. Serum APO-A, APO-B, APO-E, and Lipoprotein α (Lpα) were estimated for all participants.
Results
The annual mean concentrations of PM (PM1, PM2.5, PM10 and TSP) were lower than Egyptian limit. EPA 16 PAHs were detected in all samples, lower molecular weight PAHs were the most predominant PAHs. The most predominant wind direction was from North-West; as the selected WWPT is placed downwind to the surrounding areas. Fifty percent of the workers were complaining of dyspnea. About 30.9% of the workers were found to be hypertensive; the majority of them were from the operator department. ECG abnormalities were found in 18.3% of the workers mainly among operators (23.95%). Ventricular extra systole was the highest prevalent detected ECG abnormality followed by left ventricular enlargement. APO-B and APO-B/APO-A ratio were significantly higher in operator workers compared to the administrators and laboratory workers. While, APO-E was significantly lower in operator workers compared to the administrators and laboratory workers.
Conclusion
PM2.5 and PM1 and PAH exposure maybe associated with potential risk of developing CVD in the exposed WWTP workers. The main sources of PAHs in the WWTP could be the fuel used for the machines in the operator department, as the treatment process occurs in the WWTP contribute to high concentrations of PM and its PAHs, but the most contributed sources were found to be from the surrounding areas. The use of APO-B or APO-B/APO-A holds a promising biomarkers for evaluating cardiovascular disease risk.
Peptides Rapidly Transport Antibiotic Across the Intact Tympanic Membrane to Treat a Middle Ear Infection
Authors: Kurabi Arwa , Sereno Emily , Ryan Allen 
Published: August 2023

Abstract
The tympanic membrane (TM) forms an impermeable barrier to medical therapies for middle ear (ME) diseases like otitis media. By screening a phage-displayed peptide library, we have previously discovered rare peptides that mediate active transport of cargo across the intact membrane of animals and humans. Since the M13 filamentous bacteriophage on which the peptides are expressed are large (nearly 1 µm in length), this offers the possibility of noninvasively delivering drugs, large drug packages or gene therapy to the ME. To evaluate this possibility, EDC chemistry was employed to covalently attach amoxicillin or neomycin molecules to phage bearing a trans-TM peptide, as a model for large drug packages. Eight hours after application of antibiotic-phage to the TM of infected rats, ME bacterial titers were substantially reduced compared to untreated animals. As a control, antibiotic was linked to wild-type phage, not bearing any peptide, and application to the TM did not affect ME bacteria. The results support the ability of rare peptides to actively deliver pharmacologically relevant amounts of drugs through the intact TM and into the ME. Moreover, since bacteriophage engineered to express peptides are viral vectors, the trans-TM peptides could also transport other viral vectors into the ME.
Anti-CNTN-1 autoimmune nodopathies with dysarthria at onset - a case report and literature review
Authors: Chen Jiajie , Liu Lingchun , Zhu Hongyan , Li Rong , Li Haixia , Gong Xiarong , Fu Hao , Long Jingjing , Meng Qiang 
Published: August 2023

Abstract
BACKGROUND: Anti-CNTN-1 autoimmune nodopathies (AN) is a new and rare diagnosis of peripheral neuropathy proposed by the European Society of Neurology in recent years. Clinical manifestations usually include chronic, subacute or rapidly progressive sensory or motor disorders, sensory ataxia, tremor, and cranial nerve involvement (facial paralysis, eye movement limitation, diplopia, etc. However, anti-CNTN-1 autoimmune nodopathies with dysarthria have rarely been reported. We report a rare young case with dysarthria.
Case introduction: This is a case of a 25-year-old male patient with an initial onset of dysarthria and limb tremor. He was initially misdiagnosed withspinocerebellar ataxia (SCA), and the symptoms gradually worsened, including limb weakness, sensory disorders of the limbs, muscle atrophy of the distal lower limbs, and positional dizziness. Neuroelectrophysiological examination showed multiple peripheral nerve damage (motor and sensory damage), and the myelin sheath and axons were involved (mainly the myelin sheath). The blood and cerebrospinal fluid of peripheral neuropathy autoimmune antibody tests indicated that anti-CNTN-1 antibody, the serum GD1b antibody was positive, and a corrected diagnosis of CNTN-1 autoimmune nodopathies. The patient was treated with rituximab, and limb weakness and dysarthria were improved after 3 months of follow-up.
Conclusion: Compared with the previously reported cases of anti-CNTN-1 AN, the cases we describe are young patients with dysarthria and limb tremor, highlighting the possibility of this disease presenting as unconventional peripheral nerve onset. We hope this article will improve clinicians' awareness of this kind of disease and reduce the misdiagnosis rate of this disease to help patients diagnose and treat it in time.
Does El Niño affect MJO-AR connections over the North Pacific and associated North American precipitation?
Authors: Fernandes Laís Gonçalves , Loikith Paul C. 
Published: August 2023

Abstract
This study investigates how the positive phase of the El Niño-Southern Oscillation (EN) influences the Madden-Julian Oscillation (MJO) modulation of cool-season North Pacific atmospheric rivers (ARs) and associated AR-landfall driven precipitation over North America. EN changes the key drivers of MJO-AR connections by shifting MJO-driven convection east of 180° longitude in MJO phases 6–8 and extending the northern Pacific subtropical jet eastward. Under these conditions, the MJO tropical-extratropical teleconnection is triggered east of 180° in MJO phases 7–8, and a persistent cyclonic flow anomaly develops along the United States west coast. Anomalous northeastward integrated water vapor transport (IVT) within the cyclonic flow coupled with the MJO convection over the western (phase 7) and central (phase 8) Pacific increases AR frequency, shifting it to the east over regions that do not show a relationship with EN or MJO alone. Besides enhancing AR activity, EN background conditions increase the number of AR events, their lifetime, and mean intensity from MJO phases 6 through 8, as well as the number of MJO active days, AR initiations, and ARs making landfall over North America in MJO phases 8 − 1. The positive precipitation anomalies and increased frequency of extreme precipitation events associated with landfalling North Pacific ARs related to MJO are also shifted to the east in EN, enhancing and extending rainfall over western North America in MJO phases 6 − 1. Results provide new insight into the drivers of AR activity and associated precipitation along the west coast of North America with implications for improving subseasonal-to-seasonal predictions.
A Multi-view Breast-wise Abnormality Detection for Breast Cancer Screening using Mammography
Authors: Kebede Samuel Rahimeto , Waldamichael Fraol Gelana , Debelee Taye Girma , Aleme Muluberhan , Bedane Wubalem , Mezgebu Bethelhem , Merga Zelalem Chimdesa 
Published: August 2023

Abstract
Breast cancer has the highest incidence rate among women in Ethiopia compared to other types of cancer. Unfortunately, many cases are detected at a stage where a cure is delayed or not possible. To address this issue, mammography-based screening is widely accepted as an effective technique for early detection. However, the interpretation of mammography images requires experienced radiologists in breast imaging, a resource that is limited in Ethiopia. In this research, we have developed a model to assist radiologists in mass screening for breast abnormalities and prioritizing patients. Our approach combines an ensemble of efficient-based classifiers with YOLOv5, a suspicious mass detection method, to identify abnormalities. The inclusion of YOLOv5 detection is crucial in providing explanations for classifier predictions and improving sensitivity, particularly when the classifier fails to detect abnormalities. To further enhance the screening process, we have also incorporated an abnormality detection model. The classifier model achieves an F1-score of 0.87 and a sensitivity of 0.82. With the addition of suspicious mass detection, sensitivity increases to 0.89, albeit at the expense of a slightly lower F1-score of 0.79.
Giant extracranial internal carotid artery pseudoaneurysm : A case report
Authors: Zhang Yunwen , Ling Jun , Liu Peng , Qiu Chuanzhen , Liu Ming , Sun Tao , Mo Wei , Ouyang Yian 
Published: August 2023

Abstract
Background : Pseudoaneurysm of the internal carotid artery is a rare and dangerous disease. The normal arterial wall includes three layers: intima, media and adventitia. Pseudoaneurysms are more likely to rupture under the continuous impact of blood due to the lack of three layers of normal structure of the arterial wall. If the pseudoaneurysm is not treated in time when it ruptures, it can easily cause massive hemorrhage and endanger life.
Case presentation : We report a rare case of spontaneous extracranial giant pseudoaneurysm of the internal carotid artery in a middle-aged female. A 48-year-old woman was admitted to the hospital due to “sudden severe pain in the right mandible and neck with swelling for more than one day”. After computed tomography angiography (CTA) of the head and neck, she was diagnosed with a pseudoaneurysm of the C1 segment of the right internal carotid artery. Digital Silhouette Angiography (DSA) showed a large tumor-like dilatation in the extracranial segment of the right internal carotid artery. Because the blood vessels cannot be reconstructed, it is recommended to occlude the blood vessels or ligate the right internal carotid artery by traditional surgery. The family gave up surgical treatment and underwent conservative treatment of right common carotid artery Matas training. The patient recovered well after 1 year of follow-up.
Conclusion：The possibility of self-healing of internal carotid artery pseudoaneurysm is very small, and the possibility of rerupture is high. Therefore, patients with confirmed internal carotid artery pseudoaneurysm should be treated surgically as soon as possible to prevent the illness from worsening.
A Taxonomically-informed Mass Spectrometry Search Tool for Microbial Metabolomics Data
Authors: Dorrestein Pieter , Zuffa Simone , Schmid Robin , Bauermeister Anelize , Gomes Paulo Wender Portal , Caraballo-Rodriguez Andres , Abiead Yasin El , Aron Allegra , Gentry Emily , Zemlin Jasmine , Meehan Michael , Avalon Nicole , Cichewicz Robert , Buzun Ekaterina , Carrillo-Terrazas Marvic , Hsu Chia-Yun , Oles Renee , Ayala Adriana Vasquez , Zhao Jiaqi , Chu Hiutung , Kuijpers Mirte , Jackrel Sara , Tugizimana Fidele , Nephali Lerato , Dubery Ian , Madala Ntakadzeni , Moreira Eduarda , Costa-Lotufo Leticia , Lopes Norberto , Rezende-Teixeira Paula , Jimenez Paula , Rimal Bipin , Patterson Andrew , Traxler Matthew , Pessotti Rita de Cassia , Alvarado-Villalobos Daniel , Tamayo-Castillo Giselle , Chaverri Priscila , Escudero-Leyva Efrain , Quiros-Guerrero Luis-Manuel , Bory Alexandre , Joubert Juliette , Rutz Adriano , Wolfender Jean-Luc , Allard Pierre-Marie , Sichert Andreas , Pontrelli Sammy , Pullman Benjamin , Bandeira Nuno , Gerwick William , Gindro Katia , Massana-Codina Josep , Wagner Berenike , Forchhammer Karl , Petras Daniel , Aiosa Nicole , Garg Neha , Liebeke Manuel , Bourceau Patric , Kang Kyo Bin , Gadhavi Henna , de Carvalho Luiz Pedro , Santos Mariana Silva dos , Pérez-Lorente Alicia , Molina-Santiago Carlos , Romero Diego , Franke Raimo , Broenstrup Mark , De Leon Arturo Vera Ponce , Pope Phillip , Rosa Sabina La , Barbera Giorgia La , Roager Henrik , Laursen Martin , Hammerle Fabian , Siewert Bianka , Peintner Ursula , Licona-Cassani Cuauhtemoc , Rodriguez-Orduña Lorena , Rampler Evelyn , Hildebrand Felina , Koellensperger Gunda , Schoeny Harald , Hohenwallner Katharina , Panzenboeck Lisa , Gregor Rachel , O'Neill Ellis , Roxborough Eve , Odoi Jane , Bale Nicole , Ding Su , Damsté Jaap S. Sinninghe , Guan Xueli , Cui Jerry , Ju Kou-San , Silva Denise , Silva Fernanda , Silva Gilvan da , Koolen Hector , Grundmann Carlismari , Clement Jason , Mohimani Hosein , Broders Kirk , McPhail Kerry , Ober-Singleton Sidnee , Rath Christopher , McDonald Daniel , Knight Rob , Wang Mingxun 
Published: August 2023

Abstract
MicrobeMASST, a taxonomically-informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging a curated database of >60,000 microbial monocultures, users can search known and unknown MS/MS spectra and link them to their respective microbial producers via MS/MS fragmentation patterns. Identification of microbial-derived metabolites and relative producers, without a priori knowledge, will vastly enhance the understanding of microorganisms' role in ecology and human health.
Fabrication of Fe3O4/SiO2/Curcumin Nanohybrid Composites as New Antifibrotic Agents
Authors: Taufiq Ahmad , Nikmah Ainun , Subadra S.T.U. Intan , Sunaryono Sunaryono , Hidayat Arif , Hidayat Nurul , Susanto Hendra , Yustanti Erlina , Amrillah Tahta 
Published: August 2023

Abstract
Liver fibrosis is one of the main threats to human health. To date, antifibrosis drugs encompass several problems, such as the limited treatment effects, lack of drug targeting, and toxic side effects. To increase the therapeutic efficacy in targeting liver fibrosis, scientists should study the development of nanocarriers and effective therapeutic strategies in drug targeting for the treatment of liver fibrosis. In this study, Fe3O4/SiO2/curcumin (Cur) nanohybrid composites have been successfully synthesized and used as antifibrosis drugs in animal liver cells, which were induced with carbon tetrachloride (CCl4). The composition, shape, size, and magnetic properties of the nanocarrier contributed to the successful targeting of antifibrotic agents. Structural studies using X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy–energy dispersive X-ray, and transmission electron microscopy were also carried out. The magnetic properties of the nanocarrier to the sensitivity of the external magnetic field were tested using vibrating sample magnetometry. Furthermore, preclinical tests in the form of antibacterial and antifungal tests were performed. From these characterizations, the Fe3O4/SiO2/Cur nanohybrid composites showed good inhibition of bacterial and fungal growth. To determine the efficacy of Fe3O4/SiO2/Cur nanohybrid composites, we conducted serum glutamic oxaloacetic transaminase hytests. Notably, the Fe3O4/SiO2/Cur nanohybrid composites significantly reduced serum alanine transaminase and aspartate transaminase levels (p < 0.05). Thus, the Fe3O4/SiO2/Cur nanohybrid composites can reduce CCl4-induced liver injury.
Variations of soil metal content, soil enzyme activity and soil bacterial community in Rhododendron delavayi natural shrub forest at different elevations
Authors: Ming Tang , Li Wang , Jiyi Gong , Malik Kamran , Jie Liu , Xin Kong , Xianlei Chen , Lanlan Chen , Rong Tang , Rong Zheng , Jianfeng Wang , Yin Yi 
Published: August 2023

Abstract

Background Rhododendron delavayi is a natural shrub distributed at different elevations in the karst region of Bijie, China, and has an importance in prevention of land degradation in this region. The soil bacterial community structure of R. delavayi at three elevations (1448 m, 1643 m, 1821 m) was analyzed by high-throughput sequencing, and soil mineral elements and enzymes activities were determined. Results In this study, our results showed that the soil Fe, Mg, Cr, Ni, Cu and Zn content decreased with increase in elevations, the soil K content varied in a V-shape, while the soil Cd and Pb showed an inverted V-shape. In addition, the variation of soil urease and phosphatase activity also showed an inverted V-shape. Invertase activity increased while catalase activity decreased with elevation increase. The Shannon index of soil bacterial community initially increased and then decreased with elevation, and was highest at 1643 m. At 1821 m, Fe was positively correlated with Acidobacteria; Ca, Ni, Cu, Zn and Pb were positively correlated with Actinobacteria. Urease and phosphatase significantly affected the soil bacterial community, while elevation affected the diversity of bacterial community by affecting Fe and urease content. The results of Linear discriminant effect size (LEfSe) showed that there were different biomarkers at different elevations. The bacterial co-occurrence network analysis showed that the dominant bacterial communities at different elevations in Baili Rhododendron scenic spot had a complex structure. Acidobacteria, Actinobacteria and Proteobacteria were the most dominant phyla at 1448 m, 1643 m and 1821 m. Mantel test and correlation analysis showed that Fe and urease significantly affected bacterial OUT at 1448m. Moreover, Cr, Ni and catalase had a negative impact on TM7 at 1448m but there was no significant correlation between soil variables and bacteria communities on bacterial OUT at 1643m and 1821m. Conclusion Elevation significantly influenced structure of bacterial communities by affecting the content of soil mineral elements and the level of enzyme activity.