bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of large-scale pooled testing to increase rapid molecular diagnostic test coverage for tuberculosis: a retrospective evaluation
Authors: Vuchas Comfort , Teyim Pride , Dang Beh Frankline , Neh Angela , Keugni Liliane , Che Mercy , Che Pantalius Nji , Beloko Hamada , Fondoh Victor , Ndi Norah Nyah , Wandji Irene Adeline Goupeyou , Fundoh Mercy , Manga Henri , Mbuli Cyrille , Creswell Jacob , Bisso Annie , Donkeng Valerie , Sander Melissa 
Published: September 2023

AbstractIn 2021, only 6.4 million of the 10.6 million people with tuberculosis (TB) were diagnosed and treated for the disease. Although the World Health Organization recommends initial diagnostic testing using a rapid sensitive molecular assay, only 38% of people diagnosed with TB benefited from these, due to barriers including the high cost of available assays. Pooled testing has been used as an approach to increase testing efficiency in many resource-constrained situations, such as the COVID-19 pandemic, but it has not yet been widely adopted for TB diagnostic testing. Here we report a retrospective analysis of routine pooled testing of 10,117 sputum specimens using the Xpert MTB/RIF and Xpert MTB/RIF Ultra assays that was performed from July 2020 to February 2022. Pooled testing saved 48% of assays and enabled rapid molecular testing for 4156 additional people as compared to individual testing, with 6.6% of specimens positive for TB. From an in silico analysis, the positive percent agreement of pooled testing in pools of 3 as compared with individual testing for the Xpert MTB/RIF Ultra assay was estimated as 99.4% (95% CI, 96.6% to 100%). These results support the scale-up of pooled testing for efficient TB diagnosis.
Cross-hemispheric recruitment during action planning with increasing task demand
Authors: Schach Sonja , Braun Daniel Alexander , Lindner Axel 
Published: September 2023

AbstractThe recruitment of cross-hemispheric counterparts of lateralized prefrontal brain regions with increasing processing demand is thought to increase memory performance despite cognitive aging, but was recently reported to be present also in young adults working at their capacity limit. Here we ask if cross-hemispheric recruitment is a general strategy of the adult brain in that executive task demand would modulate bilateral activation beyond prefrontal cortex and across cognitive tasks. We analyzed data sets from two fMRI experiments investigating retrospective working memory maintenance and prospective action planning. We confirmed a cross-hemispheric recruitment of prefrontal cortex across tasks and experiments. Changes in lateralization due to planning further surfaced in the cerebellum, dorsal premotor and posterior parietal cortex. Parietal cortex thereby exhibited cross-hemispheric recruitment also during spatial but not verbal working memory maintenance. Our results confirm a domain-general role of prefrontal cortex in cross-hemispheric recruitment. They further suggest that other task-specific brain regions also recruit their idling cross-hemispheric counterparts to relocate executive processing power.
Near-field projection optical microscope (NPOM) as a new approach to nanoscale super-resolved imaging
Authors: Sanjeev Abhijit , Glukhov David , Salahudeen Rafeeka Rinsa , Karsenty Avi , Zalevsky Zeev 
Published: September 2023

AbstractA new super-resolution method, entitled Near-field Projection Optical Microscopy (NPOM), is presented. This novel technique enables the imaging of nanoscale objects without the need for surface scanning, as is usually required in existing methods such as NSOM (near-field scanning optical microscope). The main advantage of the proposed concept, besides the elimination of the need for a mechanical scanning mechanism, is that the full field of regard/view is imaged simultaneously and not point-by-point as in scanning-based techniques. Furthermore, by using compressed sensing, the number of projected patterns needed to decompose the spatial information of the inspected object can be made smaller than the obtainable points of spatial resolution. In addition to the development of mathematical formalism, this paper presents the results of a series of complementary numerical tests, using various objects and patterns, that were performed to verify the accuracy of the reconstruction capabilities. We have also performed a proof of concept experiment to support the numerical formalism.
Regulation of psoriasis, colitis, and the intestinal microbiota by clusterin
Authors: Jun Yu Kyung , Yoon Hee Tae , Kwon So Hyun , Jo Ui Hyeon , Kim Ji Eun , Han Yoo Min , Kim Min-Seon , Im Jong Pil , Lee Dong Ho , Kim Joo Sung , Koh Seong-Joon , Park Hyunsun 
Published: September 2023

AbstractPsoriasis, a chronic and systemic inflammatory disorder characterized by activation of the interleukin (IL)-23/IL-17 axis, may be associated with the intestinal microbiota through the so-called “gut–skin axis.” Clusterin is a glycoprotein ubiquitously distributed in mammalian tissues; however, its role in psoriasis is unclear. Therefore, we evaluated the role of clusterin in psoriatic skin inflammation, systemic inflammation, and colitis using a murine model of IMQ-induced psoriasis. In IMQ-treated clusterin-knockout (clusterin−/−) mice, the expressions of inflammatory cytokines in clusterin-silenced human keratinocytes and intestinal microbial composition were analyzed. We also examined clusterin expression in the skin tissues of patients with psoriasis. IMQ-induced psoriatic skin inflammation is suppressed in clusterin−/− mice. Long-term administration of IMQ induced systemic inflammation and colitis; however, both were alleviated by the genetic deletion of clusterin. Genetic silencing of clusterin in human keratinocytes inhibited the production of inflammatory cytokines involved in the initiation and progression of psoriasis. The composition of the intestinal microbiota in IMQ-treated clusterin−/− and wild-type mice was different. Genetic deletion of clusterin suppressed the increase in the Firmicutes/Bacteroidetes (F/B) ratio. Skin tissues of patients with psoriasis showed high clusterin expression. In conclusion, inhibition of clusterin decreased psoriatic skin inflammation, systemic inflammation, colitis, and altered the F/B ratio in an IMQ-induced murine psoriasis model.
Authentication of protein, fat, carbohydrates, and total energy in commercialized high protein sports foods with their labeling data
Authors: Aly Maged Ossama , Ghobashy Somia Mohamed , Aborhyem Samar Mohamed 
Published: September 2023

AbstractThe popularity of high-protein sports food items among athletes and the bodybuilding community has risen dramatically. This study aimed to authenticate the reported per serving food label content of protein, fat, carbohydrate, and total energy in commercially available high-protein sports foods available in Egyptian markets. A cross-sectional study was performed on a total of forty-five samples of nine products that included protein bars, cookies, vegan bars, puffs, whey protein, protein drinks, peanut butter, pancake mix, and Greek yogurt. Protein and fat analysis were done according to Kheldahl and Folich methods, respectively, while carbohydrate was calculated by difference. Total energy was calculated according to their content. A significant (p < 0.001) difference was found between the laboratory-assessed content and the reported food label values in protein, carbohydrate, and energy. Protein sport food products had significantly lower protein content (11.6 ± 4.67) obtained from laboratory measurement than the label reported value (17.17 ± 7.22). The fat content in vegan protein was 149.3% higher than the label values (1.67 vs. 0.67 g/serving). The mean fat content per serving of 30 out of 45 samples was significantly higher than the food label values in the bar (37.8%), puffs (32.7%), vegan protein (149.3%), and protein drinks (28.6%). These differences may result in compromised performance and undesired fat gain, as opposed to a desired increase in muscle mass, which could compromise the desired impact of the consumed sports foods.
Mycobacterium tuberculosis β-lactamase variant reduces sensitivity to ampicillin/avibactam in a zebrafish-Mycobacterium marinum model of tuberculosis
Authors: van Alen Ilona , Aguirre García Mayra A. , Maaskant Janneke J. , Kuijl Coenraad P. , Bitter Wilbert , Meijer Annemarie H. , Ubbink Marcellus 
Published: September 2023

AbstractThe β-lactamase of Mycobacterium tuberculosis, BlaC, hydrolyzes β-lactam antibiotics, hindering the use of these antibiotics for the treatment of tuberculosis. Inhibitors, such as avibactam, can reversibly inhibit the enzyme, allowing for the development of combination therapies using both antibiotic and inhibitor. However, laboratory evolution studies using Escherichia coli resulted in the discovery of single amino acid variants of BlaC that reduce the sensitivity for inhibitors or show higher catalytic efficiency against antibiotics. Here, we tested these BlaC variants under more physiological conditions using the M. marinum infection model of zebrafish, which recapitulates hallmark features of tuberculosis, including the intracellular persistence of mycobacteria in macrophages and the induction of granuloma formation. To this end, the M. tuberculosis blaC gene was integrated into the chromosome of a blaC frameshift mutant of M. marinum. Subsequently, the resulting strains were used to infect zebrafish embryos in order to test the combinatorial effect of ampicillin and avibactam. The results show that embryos infected with an M. marinum strain producing BlaC show lower infection levels after treatment than untreated embryos. Additionally, BlaC K234R showed higher infection levels after treatment than those infected with bacteria producing the wild-type enzyme, demonstrating that the zebrafish host is less sensitive to the combinatorial therapy of β-lactam antibiotic and inhibitor. These findings are of interest for future development of combination therapies to treat tuberculosis.
Calcination and ion substitution improve physicochemical and biological properties of nanohydroxyapatite for bone tissue engineering applications
Authors: Kurzyk Agata , Szwed-Georgiou Aleksandra , Pagacz Joanna , Antosik Agnieszka , Tymowicz-Grzyb Paulina , Gerle Anna , Szterner Piotr , Włodarczyk Marcin , Płociński Przemysław , Urbaniak Mateusz M. , Rudnicka Karolina , Biernat Monika 
Published: September 2023

AbstractNanohydroxyapatite (nanoHAP) is widely used in bone regeneration, but there is a need to enhance its properties to provide stimuli for cell commitment and osteoconduction. This study examines the effect of calcination at 1200 °C on the physicochemical and biological properties of nanoHAP doped with magnesium (Mg2+), strontium (Sr2+), and zinc (Zn2+). A synergistic effect of dual modification on nanoHAP biological properties was investigated. The materials were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), BET analysis, Fourier-transform spectroscopy, and thermal analysis methods. Furthermore, ion release tests and in vitro biological characterization, including cytocompatibility, reactive oxygen species production, osteoconductive potential and cell proliferation, were performed. The XRD results indicate that the ion substitution of nanoHAP has no effect on the apatite structure, and after calcination, β-tricalcium phosphate (β-TCP) is formed as an additional phase. SEM analysis showed that calcination induces the agglomeration of particles and changes in surface morphology. A decrease in the specific surface area and in the ion release rate was observed. Combining calcination and nanoHAP ion modification is beneficial for cell proliferation and osteoblast response and provide additional stimuli for cell commitment in bone regeneration.
Radiological assessment of different monazite grades after mechanical separation from black sand
Authors: kotb N. A. , Abd El Ghany M. S. , El-sayed Ashraf A. 
Published: September 2023

AbstractIn Egyptian black sands, monazite is a precious mineral characterized by its composition, which includes crucial constituents such as thorium, trace amounts of uranium, and rare earth elements. It is essential to evaluate and quantify the extent of gamma-ray exposure resulting from the presence of primordial radionuclides. This necessity arises from human activities that extract and retrieve raw materials in uranium and thorium mining operations. The current study focuses on the radiological assessment of Monazite raw material in various grades and calculates the associated hazard indices. A hyper pure Germanium detector (HPGe) determined the particular activity. For grade, 90% Monazite samples, the average activities for 232Th, 238U, and 40K were 348,008 ± 1406, 69,299 ± 2086, and 27,510 ± 245 Bq/kg, respectively. For grade 75% Monazite samples, the average activities were 219,000 ± 901, 55,000 ± 500, and 18,300 ± 86 Bq/kg, while for grade 50% Monazite samples, it was 43,294 ± 1549, 9593 ± 629, and 4000 ± 211 Bq/kg for the same element, respectively. Also, 138La’s inherent radioactivity was taken into account. The computed effective and absorbed dosages exceed the worker’s exempt limit of 20 mSv/y. The calculated hazard parameters are higher than the maximum recommended limits. Therefore, it is imperative to employ radiation safety measures to mitigate the potential hazards of ionizing radiation.
Molecular characterization of Vibrio species isolated from dairy and water samples
Authors: El-Zamkan Mona A. , Ahmed Ahmed Shaban , Abdelhafeez Hanan H. , Mohamed Hams M. A. 
Published: September 2023

AbstractVibrio species can cause foodborne infections and lead to serious gastrointestinal illnesses. The purpose of this research was to detect the Vibrio cholerae and Vibrio parahaemolyticus in raw milk, dairy products, and water samples. Also, it investigated the virulence factors, antibiotic resistance and biofilm formation in isolated bacteria. Conventional and molecular approaches were used to identify the isolates in this study. Vibrio species were detected in 5% of the samples. Vibrio cholerae and Vibrio parahaemolyticus were isolated from 1.25 and 1.5%, respectively, of the total samples. Penicillin resistance was detected in all strains of Vibrio cholerae and Vibrio parahaemolyticus, with a MAR index ranging from 0.16 to 0.5. Four isolates were moderate biofilm producer and three of them were MDR. When Vibrio cholerae was screened for virulence genes, ctxAB, hlyA, and tcpA were found in 80, 60, and 80% of isolates, respectively. However, tdh + /trh + associated-virulence genes were found in 33.3% of Vibrio parahaemolyticus isolates.
Correlate the cyanogenic potential and dry matter content of cassava roots and leaves grown in different environments
Authors: Alamu Emmanuel Oladeji , Dixon Gilbert Alfred , Adesokan Michael , Maziya-Dixon Busie 
Published: September 2023

AbstractCassava (Manihot esculenta Crantz) is an essential stable food crop in Sub-Saharan Africa commonly consumed amongst the low-income communities in Africa. Though cassava roots and leaf have vast economic and commercial benefits, it produces cyanogenic glycosides, which are toxic and most often responsible for the bitter taste of some cassava cultivars. The study evaluates the cassava roots and leaves’ cyanogenic potential and dry matter content of the Genetic Gain Assessment trial grown in a different environment. It establishes the association between the cyanogenic potential (CNP) and the roots and leaves dry matter (DM). Genetic Gain Assessment (GGA) cassava genotypes (N = 400) selected for the Uniform Yield Trial (UYT) breeding stage were planted under IVS (Dry season in Inland Valley Hydromorphic area) and Upland (rain-fed conditions) in two locations of IITA Research Farms, namely; Ibadan (IVS and Upland) and Mokwa (Upland) in Nigeria. The CNP content of cassava leaves in IVS, Mokwa, and Upland ranged from 3.39 to 272.16 mg/100 g, 4.28 to 228.72 mg/100 g, and 13.13 to 127.39 mg/100 g, respectively. However, the respective CNP range in root samples across IVS, Mokwa, and Upland was 0.76–76.31 mg/100 g, 0.94–136.53 mg/100 g, and 2.37–47.11 mg/100 g. Also, the mean ± SD of DM content of leaves were 27.97 ± 3.01%, 28.81 ± 4.01%, and 13.65 ± 3.69%, respectively, in IVS, Mokwa, and Upland, while the root samples had mean ± SD of DM content of 38.09 ± 4.80%, 32.69 ± ,5.93% and 24.63 ± 5.07% respectively. Furthermore, location and genotype had a highly significant effect (p < 0.001) on the CNP and DM of roots and leaves. Also, linear regressions were established between CNP and DM of root and leaf with regression equation; DM-Root = 1.1999*DM-Leaf (r = 0.956) and CNP-Root = 0.29006*CNP-Leaf (r = 0.54). The relationship between the DM (root and leaf) and CNP (root and leaf) could serve as a valuable “inter-prediction” tool for these parameters.
Optimal system and dynamics of optical soliton solutions for the Schamel KdV equation
Authors: Hussain A. , Chahlaoui Younes , Usman M. , Zaman F. D. , Park Choonkil 
Published: September 2023

AbstractIn this research, we investigate the integrability properties of the Schamel–Korteweg–de Vries (S-KdV) equation, which is important for understanding the effect of electron trapping in the nonlinear interaction of ion-acoustic waves. Using the optimal system, we come over reduced ordinary differential equations (ODEs). To deal with reduced ODEs for this problem, Lie symmetry analysis is combined with the modified auxiliary equation (MAE) procedure and the generalized Jacobi elliptic function expansion (JEF) method. The analytical solutions reported here are novel and have a wide range of applications in mathematical physics.
Organic walled microfossils in wet peperites from the early Cretaceous Paraná-Etendeka volcanism of Brazil
Authors: Del Mouro Lucas , Becker-Kerber Bruno , Janasi Valdecir A. , de Araújo Carvalho Marcelo , Waichel Breno L. , Lima Evandro F. , Rossetti Lucas M. M. , Cruz Vinicius , Silva Mateus Souza , Famelli Natália , Ortega-Hernández Javier 
Published: September 2023

AbstractLarge igneous provinces (LIPs) are major magmatic events that have a significant impact on the global environment and the biosphere, for example as triggers of mass extinctions. LIPs provide an excellent sedimentological and geochemical record of short but intense periods of geological activity in the past, but their contribution towards understanding ancient life is much more restricted due to the destructive nature of their igneous origin. Here, we provide the first paleontological evidence for organic walled microfossils extracted from wet peperites from the Early Cretaceous Paraná-Etendeka intertrappean deposits of the Paraná basin in Brazil. Wet peperites are a volcaniclastic rock formed by the interaction of lava and subaqueous sediments.The Paraná-Etendeka was formed during the Valanginian (ca. 132 Ma) as a continental flood basalt in present day South America and Namibia, and released enormous amounts of carbon dioxide, sulfur dioxide, methane and hydrogen fluoride into the atmosphere. The organic walled microfossils recovered from the Paraná-Etendeka peperites include pollen grains, spores, acritarchs, and other remains of unidentifiable organic matter. In addition to the peperites, organic walled microfossils were also found in heterolithic sandstones and interpillow sandstones. Our findings represent the first insight into the biodiversity of the Paraná Basin during the Early Cretaceous during a period of intense magmatism, and the microfossil assemblages corroborate a regional paleoclimatic transition from arid to more humid conditions that were likely induced by the volcanic activity. We corroborate the potential of wet peperite rocks as a valuable source of paleobiological data and emphasize the importance of sampling volcaniclastic units that have been traditionally considered with lower fossiliferous potential due to their igneous origin.
Effect of the macular shape on hole findings in idiopathic macular hole differs depending on the stage of the macular hole
Authors: Terasaki Hiroto , Yamashita Toshifumi , Funatsu Ryoh , Nomoto Shohei , Fujiwara Kazuki , Shiihara Hideki , Yamashita Takehiro , Sakamoto Taiji 
Published: September 2023

AbstractThis study aimed to investigate the relationship between macular shape and idiopathic macular hole (MH) findings using an objective method. We present retrospective observational case series on patients with MH. The shape of the macular area was quantified using quadratic equations, and the ocular shape (OS) index was calculated. The correlation between the OS index and macular hole findings for each stage was evaluated. Pearson's correlation coefficient showed a significant correlation between the OS index and horizontal hole diameter (p = 0.044), bottom diameter (p = 0.006), and vertical bottom diameter (p = 0.024) in stage 2. For stage 4, there was a negative and significant correlation between the OS index and age (p = 0.037), and horizontal (p = 0.021) and vertical (p = 0.027) bottom diameter. Multiple regression analysis showed that the horizontal (p = 0.0070) and vertical (p = 0.031) bottom diameter and OS index were independently and positively correlated in stage 2. In stage 4, the OS index was independently and negatively correlated with the horizontal (p = 0.037) and vertical (p = 0.048) bottom diameter. The ocular shape of the macula affects MH findings, and its impact depends on its stage.
Enhancement of Doppler spectroscopy to transverse direction by using optical vortex
Authors: Minagawa Hiroki , Yoshimura Shinji , Terasaka Kenichiro , Aramaki Mitsutoshi 
Published: September 2023

AbstractTunable diode laser absorption spectroscopy (TDLAS) is a valuable method for measuring particle flow velocities in plasma. However, conventional TDLAS using a plane-wave beam is sensitive only to the laser propagation direction. This limitation is particularly unfavorable for the observation of the particle transportation perpendicularly incident on the material in the plasma–material interaction. In this paper, we show for the first time that flow measurements perpendicular to the beam direction are possible by replacing the probe beam with an optical vortex beam. Because an optical vortex has a helical wavefront, particles moving in its field experience an azimuthal Doppler shift in addition to the translational Doppler shift. Assuming a uniform gas flow across the optical vortex, the azimuthal Doppler shift of the absorption spectrum observed in the beam cross-section varies sinusoidally in the azimuthal direction. The transverse flow velocity is derived from the amplitude of this sinusoidal variation. At transverse velocities above 70 m/s, the measurement errors are found to be less than 15%, with a mean absolute percentage error of less than 8%.
Experimental study on the probabilities of kinked arches and kinked arch locations in ore passes under the influences of multiple factors
Authors: Ma Jiaoyang , Wei Dianen , Zhang Qingsong 
Published: September 2023

AbstractA smooth shaft production process is important to ensure the safe and efficient development of mineral resources. Due to the influence of ore rock and the mismatch between rock size and ore pass design, an arching phenomenon occurs frequently during actual ore pass release, stagnating production. Therefore, an ore pass release simulator with a freely variable ore pass angle and height is designed and used to conduct a four-factor, five-level orthogonal ore pass release experiment. An analysis is performed to determine the influence of four factors—ore particle size, ore moisture, ore pass inclination and slip height—on the probability of ore pass arching; from there, an ore pass arching probability equation is constructed and the characteristics of the ore pass arching location are clarified, effectively predicting the probability of ore pass arching and providing an effective basis for handling arching events within an applicable range. This analysis effectively predicts the probability of ore pass arch within the applicable range, and provides an effective basis for handling ore pass arch events.