bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomal miR‐3131 derived from endothelial cells with KRAS mutation promotes EndMT by targeting PICK1 in brain arteriovenous malformations
Authors: He Qiheng , Huo Ran , Wang Jie , Xu Hongyuan , Zhao Shaozhi , Zhang Junze , Sun Yingfan , Jiao Yuming , Weng Jiancong , Zhao Jizong , Cao Yong 
Published: January 2023

AbstractAimsTo explore the underlying mechanism by which low‐frequency KRAS mutations result in extensive EndMT occurrence.MethodsExosomes derived from primarily cultured brain arteriovenous malformation (bAVMs) and human umbilical vein endothelial cells (HUVECs) transfected with KRASG12D, KRASWT, or KRASNC lentiviruses were isolated, and their effects on HUVECs were identified by western blotting and immunofluorescence staining. The expression levels of exosomal microRNAs (miRNAs) were evaluated by miRNA microarray, followed by functional experiments on miR‐3131 and detection of its downstream target, and miR‐3131 inhibitor in reversing the EndMT process induced by KRASG12D‐transfected HUVECs and bAVM endothelial cells (ECs) were explored.ResultsExosomes derived from KRASG12D bAVM ECs and KRASG12D‐transfected HUVECs promoted EndMT in HUVECs. MiR‐3131 levels were highest in the exosomes of KRASG12D‐transfected HUVECs, and HUVECs transfected with the miR‐3131 mimic acquired mesenchymal phenotypes. RNA‐seq and dual‐luciferase reporter assays revealed that PICK1 is the direct downstream target of miR‐3131. Exosomal miR‐3131 was highly expressed in KRASG12D bAVMexos compared with non‐KRAS‐mutant bAVMexos or HUVECexos. Finally, a miR‐3131 inhibitor reversed EndMT in HUVECs treated with exosomes or the supernatant of KRASG12D‐transfected HUVECs and KRASG12D bAVM ECs.ConclusionExosomal miR‐3131 promotes EndMT in KRAS‐mutant bAVMs, and miR‐3131 might be a potential biomarker and therapeutic target in KRASG12D‐mutant bAVMs.
Organizational resilience and interorganizational relationships: An exploration of Chinese business service firms
Authors: You Jacqueline Jing , Williams Christopher 
Published: January 2023

AbstractMuch research on organizational resilience has focused on the intraorganizational capacity that enables positive adjustment to disruption. Yet, when seen as open systems, organizations are highly interdependent and interconnected with many other actors. This raises the question of how interorganizational relationships (IORs) affect organizational resilience. We explore this using a novel inductive two‐stage approach incorporating fuzzy cognitive mapping to identify the relational determinants of organizational resilience in the context of Chinese business service firms. Using this technique reveals five relational dimensions, which we label relational competence, innovative assimilation, integrative trustworthiness, identity constraints, and asymmetry. The analysis also shows how these interrelate to either positively or negatively affect organizational resilience. This is a new way of understanding organizational resilience and shows how it is determined by a complex interplay between IOR attributes in the external relational environment of the organization.
Functional, genetic, and structural constraints on the exaggeration and diversification of male genital morphology inOhomopterusground beetles
Authors: Terada Karen , Takahashi Sougo , Takami Yasuoki 
Published: January 2023

AbstractThe evolution of exaggerated sexual traits may be possible by the relaxation of various constraints on exaggeration. Functional constraints refer to the reduced performance of exaggerated traitsper seor increased survival costs by holding the exaggerated traits. Genetic constraints, such as genetic correlations or pleiotropy, may hinder the independent evolution and exaggeration of traits. Structural constraints, such as competition for space and resources among traits, may require the coordination of the exaggerated trait with surrounding structures. The remarkable diversity of male genital morphology provides an ideal opportunity for examining constraints on sexual trait exaggeration. In this study, we addressed the constraints on the evolution of exaggerated male genital morphology based on a comparative analysis of phenotypic covariation between the genitalia and other body parts usingOhomopterusground beetles. We found that exaggerated male genitalia were related to a relaxation of functional constraint, as revealed by a steeper allometric slope in the species with exaggerated male genitalia. By contrast, genetic constraint based on a shared genetic basis for the male genitalia and other appendages may have little effect on diversification in male genitalia. Structural constraints were strongest in the species with the most exaggerated male genitalia, suggesting that the observed constraint was a result of exaggeration. These findings improve our understanding of sexual trait exaggeration and underlying constraints.
Morphological and sensorimotor phenotypes in a zebrafish CHARGE syndrome model are domain‐dependent
Authors: Hodorovich Dana R. , Lindsley Patrick M. , Berry Austen A. , Burton Derek F. , Marsden Kurt C. 
Published: January 2023

AbstractCHARGE syndrome is a heterogeneous disorder characterized by a spectrum of defects affecting multiple tissues and behavioral difficulties such as autism, attention‐deficit/hyperactivity disorder, obsessive–compulsive disorder, anxiety, and sensory deficits. Most CHARGE cases arise from de novo, loss‐of‐function mutations in chromodomain‐helicase‐DNA‐binding‐protein‐7 (CHD7). CHD7 is required for processes such as neuronal differentiation and neural crest cell migration, but how CHD7 affects neural circuit function to regulate behavior is unclear. To investigate the pathophysiology of behavioral symptoms in CHARGE, we established a mutant chd7 zebrafish line that recapitulates multiple CHARGE phenotypes including ear, cardiac, and craniofacial defects. Using a panel of behavioral assays, we found that chd7 mutants have specific auditory and visual behavior deficits that are independent of defects in sensory structures. Mauthner cell‐dependent short‐latency acoustic startle responses are normal in chd7 mutants, while Mauthner‐independent long‐latency responses are reduced. Responses to sudden decreases in light are also reduced in mutants, while responses to sudden increases in light are normal, suggesting that the retinal OFF pathway may be affected. Furthermore, by analyzing multiple chd7 alleles we observed that the penetrance of morphological and behavioral phenotypes is influenced by genetic background but that it also depends on the mutation location, with a chromodomain mutation causing the highest penetrance. This pattern is consistent with analysis of a CHARGE patient dataset in which symptom penetrance was highest in subjects with mutations in the CHD7 chromodomains. These results provide new insight into the heterogeneity of CHARGE and will inform future work to define CHD7‐dependent neurobehavioral mechanisms.
‘I felt alive’: A qualitative study of inpatients' perspectives on good mental health nursing in South Korea
Authors: Ahn Suyoun , Shin Soyoung 
Published: January 2023

AbstractAlthough mental health nurses continuously strive for patients' well‐being, patients still experience inpatient care negatively. To identify an individual's actual, mental health nursing care needs and offers them optimal benefits during their hospital stay, determining what patients perceive as good nursing should be prioritized. This qualitative descriptive study aimed to understand good mental health nursing from the inpatients' perspective. The data were collected through semi‐structured interviews with 13 patients admitted to a psychiatric ward in South Korea, and analysed using thematic analysis. The Consolidated Criteria for Reporting Qualitative Research (COREQ) guidelines were used to ensure transparency and rigour. The results revealed that patients experienced good mental health nursing when nurses respected their humanity, adopted a practical approach to solve problems pragmatically, and facilitated their recovery. These practices stem from mental health nurses' attitudes towards humanity based on individualized respect, professional ability to handle patients' individual needs, and offering patients hope and confidence for recovery and the future. Therefore, mental health nurses need to focus their efforts on these aspects of nursing attitudes and competencies to ensure that inpatients experience good mental health nursing.
RETRACTED: Thoughts and experiences regarding leg amputation among patients with diabetic foot ulcers: A phenomenological study
Authors: Yip Ka‐Huen , Yip Yuk‐Chiu , Tsui Wai‐King 
Published: January 2023

AbstractDespite lower‐limb amputation being common among patients with diabetic foot ulcers, few studies have qualitatively investigated the patients' perspectives. Therefore, this study aimed to explore the thoughts and experiences of patients with diabetic foot ulcers regarding lower‐limb amputation in Hong Kong. A phenomenological study using individual, semi‐structured interviews was conducted with 18 participants with foot ulcers recruited in Hong Kong between July and September 2022. The interviews were audio‐recorded, transcribed verbatim, and analysed using an interpretative phenomenological approach and a constant comparison strategy. The results highlighted four substantial themes: (a) coping or being alone and taciturn, (b) altered appearance impacting one's sense of maintaining social relations, (c) thoughts about self‐efficacy and encountering a new normal, and (d) the possibility of a reduced gap in physical consequences between the old and new self. This study provided different perspectives of patients with a history of diabetic foot ulcers, even in cases where lower‐limb amputation has not yet been performed. The results demonstrate that lower‐limb amputation is considered a forbidden topic. This makes it culturally difficult for Chinese patients to discuss the matter with healthcare authorities and family members. Healthcare workers should be aware of how they communicate regarding lower‐limb amputation.
Exploring the microstructure of hydrated collagen hydrogels under scanning electron microscopy
Authors: Merryweather Daniel J. , Weston Nicola , Roe Jordan , Parmenter Christopher , Lewis Mark P. , Roach Paul 
Published: January 2023

AbstractCollagen hydrogels are a rapidly expanding platform in bioengineering and soft materials engineering for novel applications focused on medical therapeutics, medical devices and biosensors. Observations linking microstructure to material properties and function enables rational design strategies to control this space. Visualisation of the microscale organisation of these soft hydrated materials presents unique technical challenges due to the relationship between hydration and the molecular organisation of a collagen gel. Scanning electron microscopy is a robust tool widely employed to visualise and explore materials on the microscale. However, investigation of collagen gel microstructure is difficult without imparting structural changes during preparation and/or observation. Electrons are poorly propagated within liquid‐phase materials, limiting the ability of electron microscopy to interrogate hydrated gels. Sample preparation techniques to remove water induce artefactual changes in material microstructure particularly in complex materials such as collagen, highlighting a critical need to develop robust material handling protocols for the imaging of collagen hydrogels. Here a collagen hydrogel is fabricated, and the gel state explored under high‐vacuum (10−6 Pa) and low‐vacuum (80–120 Pa) conditions, and in an environmental SEM chamber. Visualisation of collagen fibres is found to be dependent on the degree of sample hydration, with higher imaging chamber pressures and humidity resulting in decreased feature fidelity. Reduction of imaging chamber pressure is used to induce evaporation of gel water content, revealing collagen fibres of significantly larger diameter than observed in samples dehydrated prior to imaging. Rapid freezing and cryogenic handling of the gel material is found to retain a porous 3D structure following sublimation of the gel water content. Comparative analysis of collagen hydrogel materials demonstrates the care needed when preparing hydrogel samples for electron microscopy.
Role of HIF‐1α in pathogenic mechanisms of keloids
Authors: Qiu Zi‐kai , Zhang Ming‐zi , Zhang Wen‐chao , Li Zhi‐jin , Si Lou‐bin , Long Xiao , Yu Nan‐ze , Wang Xiao‐jun 
Published: January 2023

AbstractBackgrouds and ObjectiveKeloids are defined as overrepairing products that develop after skin lesions. Keloids are characterized by the proliferation of fibroblasts and the overaccumulation of extracellular matrix components (mainly collagen), leading to a locally hypoxic microenvironment. Hence, this article was aimed to review hypoxia in pathogenesis of keloids.MethodsWe reviewed and summarized the relevant published studies.ResultsHypoxia results in the accumulation of hypoxia‐inducible factor 1α (HIF‐1α) in keloids, contributing to overactivation of the fibrotic signaling pathway, epithelial–mesenchymal transition, and changes in metabolism, eventually leading to aggravated fibrosis, infiltrative growth, and radiotherapy resistance.ConclusionIt is, therefore, essential to understand the role of HIF‐1α in the pathogenic mechanisms of keloids in order to develop new therapeutic approaches.
Facial psoriasis in the etiology of prolonged erythema following medium‐depth chemical peels for severe photodamage
Authors: Leow Liang Joo , Zubrzycki Nicolas 
Published: January 2023

AbstractBackgroundChemical peeling is the controlled wounding of the epidermis and dermis for skin rejuvenation, involving the application of ablative agents to induce keratolysis and regeneration of damaged cell layers. Prolonged erythema is one complication of this procedure. We report the prevalence and probable etiology of prolonged facial erythema in a cohort of patients treated with medium‐depth chemical peels.Materials and MethodsA retrospective audit was conducted of all medium‐depth facial chemical peels performed at two major teaching hospitals. All patients had severe facial photodamage affecting at least 75% surface area of the face. The occurrence of prolonged erythema following this peel was then identified and analyzed.ResultsOf our treatment cohort (n = 82, 51 women, 31 men) with 60 years mean (61.3 years for women, 56.7 years for men), 10 patients (12%; eight women, two men) experienced prolonged erythema beyond a month of treatment. Facial psoriasis was not apparent at the time of chemical peel but manifested as prolonged erythema beyond the expected timeframe following the procedure.ConclusionWhen patients experience prolonged erythema beyond a month of treatment and fail to respond to standard treatments, clinicians should examine carefully for extra‐facial psoriasis prior to this procedure, and also consider facial psoriasis a possible cause of prolonged post‐peel erythema.
Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23
Authors: Määttä Arto , Nixon Rebecca , Robinson Neil , Ambler Carrie A. , Goncalves Kirsty , Maltman Victoria , Przyborski Stefan 
Published: January 2023

AbstractBackgroundRetinoid signaling is an important regulator of the epidermis and skin appendages. Therefore, synthetic retinoids have been developed for therapeutic use for skin disorders such as psoriasis and acne.AimsIn previous studies, we showed how the photostable retinoid EC23 induces neuronal differentiation in stem cell‐like cell populations, and here, we aim to investigate its ability to influence epidermal and hair follicle growth.MethodsEC23 influence on skin biology was investigated initially in cultures of monolayer keratinocytes and three‐dimentional in vitro models of skin, and finally in in vivo studies of mice back skin.ResultsEC23 induces keratinocyte hyperproliferation in vitro and in vivo, and when applied to mouse skin increases the number of involucrin‐positive suprabasal cell layers. These phenotypic changes are similar in skin treated with the natural retinoid all‐trans retinoic acid (ATRA); however, EC23 is more potent; a tenfold lower dose of EC23 is sufficient to induce epidermal thickening, and resulting hyperproliferation is sustained for a longer time period after first dose. EC23 treatment resulted in a disorganized stratum corneum, reduced cell surface lipids and compromised barrier, similar to ATRA treatment. However, EC23 induces a rapid telogen to anagen transition and hair re‐growth in 6‐week‐old mice with synchronously resting back skin follicles. The impact of EC23 on the hair cycle was surprising as similar results have not been seen with ATRA.ConclusionsThese data suggest that synthetic retinoid EC23 is a useful tool in exploring the turnover and differentiation of cells and has a potent effect on skin physiology.
Mussel adhesive protein treatment delivered by microneedling for sensitive skin: A clinical study
Authors: Dong Richeng , Jin Qingmei , Zhi Jiahui , Luo Yinli , Yuan Jiachen , Pi Longquan , Nan Meilan , Jin Zhehu , Jin Chenglong 
Published: January 2023

AbstractBackgroundMussel adhesive protein (MAP) is extracted from the mycelial glands of marine mussels. It has anti‐inflammatory properties and may relieve skin itching and other symptoms.AimsBased on the anti‐inflammatory effect of MAP, this study was designed to treat sensitive skin (SS) using MAP delivered by skin microneedling.Patients/methodsTwenty‐three Chinese female patients with SS were enrolled. Treatments were delivered three times at one‐month intervals. Symptom improvement and recurrence rates, treatment safety, and patient satisfaction levels were evaluated.ResultsAfter one course of treatment, 20 patients had a Symptom Score Reducing Index (SSRI) of >20%, with an effectiveness rate of 87%. At the end of treatment, all patients had an SSRI of >20%, and the effectiveness rate was 100%. Dryness, tightness, desquamation, flushing, burning, itching, and tingling improved. After treatment, the Clinical Erythema Assessment and Lesion Severity Index of Facial Telangiectasia scores were significantly decreased. Clinical photographs following treatment revealed improved erythema reaction and decreased capillary density. During treatment, the patients experienced mild pain and erythema and swelling reaction without exudation. Complications, such as pigmentation changes or scarring, were absent. Additionally, there were no cases of recurrence, and patient satisfaction levels were high.ConclusionMAP combined with microneedling can help treat SS, showing satisfactory safety outcomes and high patient satisfaction.
Racial Differences in Black and White Residential Outcomes in the Sundown Era
Authors: Tobias‐Lauerman Abigail 
Published: January 2023

AbstractUsing publicly available census and historical records, I compare the Sundown‐era residential patterns and outcomes of Black and White residents from one small Wisconsin city between 1880 and 1930 to observe how sundown violence may have affected Black residential outcomes. Census summary data shows that while the White racial group population continued to grow at the state, county, and city levels, the Black population at the city level stalled before dropping to zero, providing evidence for sundown‐type violence and exclusion against Black households by White city residents. For the Black residents (N = 18) whose histories I could trace, three outcomes were observed: remaining in the city, internally migrating to an adjacent county, and moving to much larger metropolitan areas that were already known as Black residential destinations. In contrast, the residential outcomes for White residents (n = 42) were much more varied in their residential destinations, both at the state/regional levels, and in the size of community settled in. I suggest that sundown‐era displacement should be further considered in discussions of Black internal migration in the early 20th century, and that residents of formerly sundown towns and cities need to confront their under‐examined histories of racial exclusion.
Revisiting Religious Ethics as Field and Discipline
Authors: Herdt Jennifer A. 
Published: January 2023

ABSTRACTReturning to John P. Reeder's 1978 essay on “Religious Ethics as a Field and Discipline,” this essay explores debates surrounding the original intentions for the Journal of Religious Ethics (JRE) and for the field of religious ethics, as these have played out over the decades among an influential group of scholars involved with the JRE since its inception: Arthur Dyck, Ronald Green, Stanley Hauerwas, and Jeffrey Stout. While the JRE and its founding mission are in need of ongoing critique and transformation, we might well still affirm the journal's ongoing importance as a site for the negotiation of a commons, the identification of possibilities for shared life, imagined beyond exclusion and domination.
Phylogeography and introgression between Pinus kesiya and Pinus yunnanensis in Southeast Asia
Authors: Gao Jie , Tomlinson Kyle W. , Zhao Wei , Wang Baosheng , Lapuz Ralph Sedricke , Liu Jing‐Xin , Pasion Bonifacio O. , Hai Bach T. , Chanthayod Souvick , Chen Jin , Wang Xiao‐Ru 
Published: January 2023

AbstractSoutheast Asia (SEA) has seen strong climatic oscillations and fluctuations in sea levels during the Quaternary. The impact of past climate changes on the evolution and distribution of local flora in SEA is still poorly understood. Here we aim to infer how the Quaternary climate change affects the evolutionary process and range shifts in two pine species. We investigated the population genetic structure and diversity using cytoplasmic DNA markers, and performed ecological niche modeling to reconstruct the species past distribution and to project range shift under future climates. We found substantial gene flow across the continuous distribution of the subtropical Pinus yunnanensis. In contrast, the tropical Pinus kesiya showed a strong population structure in accordance with its disjunct distribution across montane islands in Indochina and the Philippines. A broad hybrid zone of the two species occurs in southern Yunnan. Asymmetric introgression from the two species was detected in this zone with dominant mitochondrial gene flow from P. yunnanensis and chloroplast gene flow from P. kesiya. The observed population structure suggests a typical postglaciation expansion in P. yunnanensis, and a glacial expansion and interglacial contraction in P. kesiya. Ecological niche modeling supports the inferred demographic history and predicts a decrease in range size for P. kesiya under future climates. Our results suggest that tropical pine species in SEA have undergone evolutionary trajectories different from high latitude species related to their Quaternary climate histories. We also illustrate the need for urgent conservation actions in this fragmented landscape.