bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis
Authors: Rudman-Melnick Valeria , Adam Mike , Stowers Kaitlynn , Potter Andrew , Ma Qing , Chokshi Saagar M. , Vanhoutte Davy , Valiente-Alandi Iñigo , Lindquist Diana M. , Nieman Michelle L. , Kofron J. Matthew , Potter S. Steven , Devarajan Prasad 
Published: May 2023

Abstract
Examining kidney fibrosis is crucial for mechanistic understanding and developing targeted strategies against chronic kidney disease (CKD). Persistent fibroblast activation and tubular epithelial cell (TEC) injury are key CKD contributors. However, cellular and transcriptional landscapes of CKD and specific activated kidney fibroblast clusters remain elusive. Here, we analyzed single cell transcriptomic profiles of two clinically relevant kidney fibrosis models which induced robust kidney parenchymal remodeling. We dissected the molecular and cellular landscapes of kidney stroma and newly identified three distinctive fibroblast clusters with “secretory”, “contractile” and “vascular” transcriptional enrichments. Also, both injuries generated failed repair TECs (frTECs) characterized by decline of mature epithelial markers and elevation of stromal and injury markers. Notably, frTECs shared transcriptional identity with distal nephron segments of the embryonic kidney. Moreover, we identified that both models exhibited robust and previously unrecognized distal spatial pattern of TEC injury, outlined by persistent elevation of renal TEC injury markers including Krt8, while the surviving proximal tubules (PTs) showed restored transcriptional signature. Furthermore, we found that long-term kidney injuries activated a prominent nephrogenic signature, including Sox4 and Hox gene elevation, which prevailed in the distal tubular segments. Our findings might advance understanding of and targeted intervention in fibrotic kidney disease.
Long-term Efficacy of Anti-IL-4 Receptor Antibody in a Patient With Aspirin-Exacerbated Respiratory Disease and IgG4-Related Disease
Authors: Jeon Hyun-Seob , Jang Jae-Hyuk , Lee Youngsoo , Park Hae-Sim 
Published: May 2023

Abstract
Background
Aspirin-exacerbated respiratory disease (AERD) and IgG4-related disease (IgG4RD) share a common pathway of Th2-mediated immune mechanism; there have been several cases of IgG4RD developed in patients with asthma, especially in those comorbid with chronic rhinosinusitis (CRS). IgG4RD has often been treated with systemic corticosteroids, rituximab, or immune-suppressive agents, but frequently failed with relapse.
Case presentation:
Here, we present a case of a 64-year-old male patient with severe AERD with CRS complicated with IgG4RD, who has been successfully treated and maintained with anti-IL-4 receptor antibody, dupilumab after achieving unsatisfactory responses with previous treatments including steroids, rituximab, omalizumab, and reslizumab. The patient’s symptoms (periorbital swelling and asthmatic/nasal symptoms) were remarkably improved; serum levels of IgG4/IgE as well as plasmablast/eosinophil counts progressively decreased without any recurrence sign for over 2 years of dupilumab treatment.
Conclusion
These findings demonstrate that blocking the IL-4/IL-13 pathway with dupilumab can be an effective treatment with long-term safety in patients with severe AERD with CRS complicated by IgG4RD.
Derivation of trophoblast stem cells unveils unrestrained potential of mouse embryonic stem cells and epiblast
Authors: Jana Debabrata , Singh Priya , Sailasree Purnima , Srivast Mansi , Kumar Nithyapriya , Vishnu Vijay V , Kale Hanuman T , Lakshmi Jyothi , Srini Gunda , Sowpati Divya Tej , Shekar P Chandra 
Published: May 2023

Abstract
Mouse epiblast and embryonic stem cells (mESCs) are considered to adhere strict lineage restriction and lack the potential to contribute to trophoectoderm (TE). In contrast, here we report the derivation of trophoblast stem cells (ESTS) from the mESCs. The single-cell transcriptome and molecular characterization of ESTS show similarity with TSCs. They efficiently integrate into the TE compartment of the blastocyst and contribute to the placenta during development. We discovered GSK3b signaling as the primary barrier for TE potential of the ESCs. It plays a vital stage-specific function during ESTS derivation. b-CATENIN and an intron-I regulatory element of Cdx2 were found to be essential for priming the ESCs to TE fate. We further show that the mouse epiblast possesses developmental plasticity and can readily differentiate into TE lineage. In contrast to the paradigm of the restricted potential of pluripotent ESCs and epiblast, our data shows that murine ESCs and epiblast have the unrestrained developmental potential for extraembryonic lineages.
Annealing determined β-phase polypropylene crystal texture, separator porous channels after biaxial stretching, and lithium-ion battery performances
Authors: Ding Lei , Li Dandan , Zhang Sihang , Du Fanghui , Zhang Daoxin , Yang Feng 
Published: May 2023

Abstract
Uneven porous channels tend to undergo structure-determined chemical deterioration as lithium-ion battery (LIB) operates, which may restrict lithium-ion migration behaviors within the separator and deteriorate cell performances. This research precisely regulates β-phase polypropylene crystal texture and porous channels after biaxial stretching based on the systematic annealing process to explore annealing decided separator porous channels and subsequent electrochemical performances of LIB. Suitable annealing temperature maximally concentrates lamellae layer dispersion and enhances thermal stability, which homogenizes biaxial tensile deformation and subsequent porous channels. Identical crystal and porous channel optimizations are also achieved by synchronously rising annealing temperature and shortening annealing time, especially annealing at 155℃ for only 10s, which lowers the Li+ migration barrier and strengthens cell C-rate and cycling stability. This desirable improvement does not require the sacrifice of time cost to achieve, which verifies separator structure-chemical affected LIB performance and the application feasibility of annealing in the actual production of the dry double-drawn separator.
Potential use of artichoke on melanoma treatment
Authors: Mathew Annette , Deng Zuliang , Nelson Christian , Mayberry Trenton G , Bai Qian , Lequio Marco , Fajardo Emerson , Xiao Huaping , Wakefield Mark , Fang Yujiang 
Published: May 2023

Abstract
Background
Melanoma is the most lethal malignancy in skin cancers. About 97,610 new cases of melanoma are projected to occur in the US in 2023. Artichoke is a very popular plant widely consumed in the US due to its nutrition. In recent years, it has been shown that artichoke shows powerful anti-cancer effects on cancers such as breast cancer, colon cancer, liver cancer and leukemia. However, there is little known about its effect on melanoma. This study was designed to investigate if artichoke extract (AE) has any direct effect on the growth of melanoma.
Methods
Clonogenic survival assay, cell proliferation, and caspase-3 activity kits were used to evaluate the effects AE on cell survival, proliferation, and apoptosis of the widely-studied melanoma cell line HTB-72. We further investigated the possible molecular mechanisms using RT-PCR and immunohistochemical staining.
Results
The percentage of colonies of HTB-72 melanoma cells decreased significantly after treated with AE. This was paralleled with the decrease in the optic density (OD) value of cancer cells after treated with AE. This was further supported by the decreased expression of PCNA mRNA after treated with AE. Furthermore, the cellular caspase-3 activity increased after treated with AE. The anti-proliferative effect of AE on melanoma cells correlated with increased p21, p27 and decreased cdk4. The pro-apoptotic effect of AE on melanoma cells correlated with decreased survivin.
Conclusions
Artichoke inhibits growth of melanoma by inhibition of proliferation and promotion of apoptosis. Such a study might be helpful to develop a new promising treatment for melanoma.
Effects of three kinds of different protein-to-fat ratio ingredi-ents on growth, slaughter performance and fat deposition of Small-Tail Han lambs
Authors: Li Qin , Yang Dong , Yan Tu , Ma Tao , Du Xinmin , Ruijun Liu , Zhang Jianxin , Diao Qiyu 
Published: May 2023

Abstract
This study aimed to investigate the effect of three kinds of different protein-to-fat ratio ingredients on growth, slaughter performance and meat quality of Small-tail Han lambs. For this, 45 Small-tailed Han lambs (♂)(BW = 34.00 ± 2.5 kg, age = 120 ± 9 d) were randomly assigned to three treatment: (1) PF5 group, (2) PF10 group and (3) PF20 group, each treatment consisted of fifteen repeats. The ratio of protein and fat in three ingredients were the PF5 (CP:EE = 50:5 ), PF10 (CP:EE = 50:10), and PF20 (CP:EE = 50:20 ). This experiment lasted for 65 days, with feed intake recorded daily, and animals being weighed on days 0, 30, and 65. At the end of the experiment, eight lambs from each group were slaughtered, slaughter performance and meat quality were subsequently determined. The results showed that the PF10 group had the highest average daily weight gain (370.15 g) (P < 0.05). Fore-gastric weights (1216.88 g) of the PF10 group were significantly highest the other groups (P < 0.05). There was no significant difference in the proportion of fat weight to body weight in different parts, which was basically at the same level (P > 0.05). Feed conversion rate of the PF10 group was also the best. Furthermore, the PF10 group had a high carcass weight and slaughter rate and there were few differences between the other groups in terms of dry matter intake, meat quality, organ weight, and fat deposition (P > 0.05). In conclusion, a protein-fat ratio of raw materials of 50:10 is the best ratio, which can more conducive to enhancing the growth and development of small-tail Han lambs.
Study on the Influence of Desert Highway Corridor on Sand Dune Mobility Rate
Authors: Wang Fang , Liu Shixiao 
Published: May 2023

Abstract
In order to more conveniently explore the movement rate of the Tengger desert dunes and analyze the influencing factors, this paper takes the Tengger desert dunes as the research object, starting from the dune ecology of the highway corridor,the influence of the construction of corridor on dune movement is explored by using UAV tilt photography technology. The results found that after the construction of the desert highway corridor, the movement rates of the leeward side with protection, the windward side with protection, the leeward side without protection and the windward side without protection are 1.13 m/a, 1.40 m/a, 1.47 m/a and 1.94 m/a, respectively. After the construction, the movement rates of the dunes decrease by 48.17%, 35.78%, 20.18% and 11.18%, respectively.The UAV tilt photography technology can provide a more convenient monitoring services for the sand dune migration at a larger scale, and the research results can provide a reference for the characteristics of the sand dune migration characteristics in similar areas.
HOXB2 Is a Prognostic Biomarker and Correlated with Immune Infiltration in Colorectal Cancer and Glioma
Authors: Liu Zhenrui , Wen Puqiao , Wang Shuxing , Liao Yuxuan , Zhou Dawei , Wang Hongyi , Xu Ruofan , Chen Yiwei 
Published: May 2023

Abstract
Homeobox B2 (HOXB2) is a transcription factor that belongs to the homeobox family. Previous investigations have demonstrated that tumor prognosis was strongly linked to aberrant HOXB2 expression. However, the mechanism linking HOXB2 to the tumor microenvironment (TME) and prognosis has not been explored in detail.
Three cohorts at different stages of colorectal cancer (GSE17536, GSE17537, GSE14333) demonstrated that elevated HOXB2 expression had significantly association with poorer overall survival (OS), disease-specific survival (DSS), and disease-free survival (DFS). Additionally, the poor prognosis in low grade glioma (LGG) (OS HR = 1.8, P-value = 0.00084; DFS HR = 1.5, P-value = 0.014) was associated with increased HOXB2 expression. Moreover, we also demonstrated that the correlation between HOXB2 expression with prognosis of gastric cancer, breast cancer, and liver cancer. What’s more, the results analyzed by TIMER further showed that HOXB2 expression was associated with immune infiltration level of colon adenocarcinoma (COAD), rectum adenocarcinoma (READ) and LGG, particularly B cells, CD4+ T cells, CD8+ cells. macrophages, neutrophils, and dendritic cells (DCs). The positive correlation between HOXB2 expression and the expression of immune maker genes was also observed. The results of RT-qPCR were reliable and we supposed that HOXB2 expressing validation provided strong evidence for HOXB2 functioning as a novel prognosis biomarker.
These findings suggested that poor prognosis and increased immune infiltration levels were found to be correlated with increased HOXB2 expression in multiple cancers, especially in COAD, READ and LGG. Furthermore, in COAD, LGG and READ, HOXB2 potentially regulates TAMs, DCs, T cell exhaustion, and Tregs. Therefore, HOXB2 can serve as a biomarker with special prognostic value and may be involved in the regulation of immune cell infiltration in patients with COAD, READ and LGG.
Metagenomic insight into the microbial degradation of biomass and alkaloids in the aging process of cigar
Authors: Xue Fang , Yang Juan , Luo Cheng , Li Dongliang , Shi Guiyang , Song Guangfu , Li Youran 
Published: May 2023

Abstract
The aging process is the final step in cigar production and plays a vital role in the quality. There are complex microbial growth and metabolism, enzymatic catalysis, and chemical reactions in this process. Among them, microorganisms function as a bridge between organic and inorganic contents. At present, we do not yet have sufficient knowledge of the evolution of microorganisms in the aging process of cigars. Neither are we clear about the mechanisms by which environmental conditions may impact the quality of cigars through the interaction of microorganisms and chemical substances. In this study, we used a metagenomics sequence-guided strategy to identify the microbes and enzymes involved in the degradation of key compounds, biomass and alkaloids, under different environmental conditions. The results showed that different environmental factors including temperature, humidity, aging cycle, and turning frequency resulted varied contents of total sugar, and alkaloids in cigars. Microbial communities showed significant correlations with starch, reducing sugars, total sugars and alkaloids. Vital species in the catabolism of starch (Bacillus pumilus, Pseudomonas sp. 286 and Aspergillus cristatus), reducing sugars and total sugars (Aspergillus cristatus and Nitrolancea hollandica) were identified. Corynespora cassiicola and Pseudomonas fulva were proposed to contribute to the degradation of nornicotine and neonicotinoid. Our work should deepen the understanding of microbial roles in cigar aging and provide a new viewpoint for applying specific microbial consortia to modulate the degradation of biomass and alkaloids in cigar leaves.
Inter-provincial Carbon Emission Transfer Paths, Spatial and Temporal Characteristics and Influencing Factors in China from a Value Perspective
Authors: Liu Hongguang , Dong Xiaocui 
Published: May 2023

Abstract
Carbon emission reduction is an arduous task that needs to be faced and solved globally at present and in the future period, and it is also an important goal in the process of China's economic and social development, and it is crucial to study carbon emissions based on value chain transfer to achieve carbon emission reduction and scientific and reasonable allocation of carbon emission reduction responsibilities. Based on the input-output method, this paper applies the value-based carbon emission accounting method to calculate the carbon emissions, net transfer of carbon emissions and net transfer growth rate of 30 provinces, autonomous regions and cities (excluding Tibet, Hong Kong, Macao and Taiwan) in China in 2012 and 2017, and analyzes the carbon emission transfer characteristics between provinces in China. The results show that during 2012–2017, China's carbon emissions as a whole showed a shift from regions with a higher level of economic development to regions with a lower level of economic development, from the east to the west, and the net transfer out center of gravity shifted significantly to the north and to the west. The main carbon emission transfer in and out of each province and city have changed, and the central region has always been the region with the most transferred carbon emissions, but the net transfer growth rate between some provinces in the central region and other regions has decreased more, and the net transfer growth rate between the northeast and western regions and all provinces and cities in the country, especially those in the coastal region, are larger. The level of economic development and industrial structure contribute the most to the change of net transfer of carbon emissions, and the population factor contributes the least. The results of this paper will provide a basis for improving carbon transfer research in China and guiding the formulation and implementation of carbon emission reduction policies.
MicroRNA-499-5p inhibits Transforming Growth Factor β1-Induced Smad Signaling pathway and suppresses atrial fibroblasts in rat by targeting TGFβ-R1
Authors: Zhao Qing , Yang Wentao , Li Xiangdong , Yuan Hongtao , Guo Jianping , Wang Yutang , Shan Zhaoliang 
Published: May 2023

Abstract
Background
Artial fibrosis has been recognized as a typical pathological change in atrial fibrillation (AF). Although present evidence suggests that microRNA-499-5p (miR-499-5p) plays an important role in the development of atrial fibrosis, the specific mechanism is not fully understood. Therefore, this study attempted to assess the influence of miR-499-5p on atrial fibroblasts and explore the potential molecular mechanism.
Methods
Atrial fibroblasts from Sprague Dawley (SD) rat were respectively transfected with miR-499-5p mimics, miR-499-5p negative control and miR-499-5p inhibitors, atrial fibroblasts without any treatment were also established. Cell counting kit-8 (CCK-8) assay and Transwell assay were used to detect the proliferation and migration of atrial fibroblasts in each group. Expressions of miR-499-5p, TGFβ1, smad2, a-SMA, collagen-I and TGFβ-R1 in mRNA and protein level were subsequently detected via Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and western blot. Furthermore, the prediction of the binding sites of miR-499-5p and TGFβ-R1 was performed via the bioinformatics online software TargetScan and verified by Dual Luciferase Reporter.
Results
By utilizing miR-499-5p-transfected atrial fibroblasts model, expression of miR-499-5p in the miR-499-5p mimics group was upregulated, while it was downregulated in the miR-499-5p inhibitors group. Upregulated miR-499-5p expression led to to a significant decrease in the proliferative and migratory ability of cultured atrial fibroblasts, while downregulated miR-499-5p expression led to a significant increase in the proliferative and migratory ability of cultured atrial fibroblasts. Additionally, upregulated miR-499-5p expression made a significant rise in TGF-β1-induced mRNA and protein expression of TGFβ1, TGFβ-R1, smad2, a-SMA and collagen-I in atrial fibroblasts. Furthermore, results from the Dual Luciferase Reporter conformed that miR-499-5p may repress TGFβ-R1 by binding the 3'UTR of TGFβ-R1 directly.
Conclusions
MiR-499-5p is able to inhibit the activation of Transforming Growth Factor β-Induced Smad Signaling and eventually suppressed the proliferation, migration and invasion of atrial fibroblasts by targeting TGFβ-R1.
Mesenchymal stem cell-derived extracellular vesicles for treatment of chronic kidney disease in preclinical animal models: a meta-analysis
Authors: Wang Hongshuang , Liu Jiazhi , Fang Fang , Gao Lanjun , Zhao Chenchen , Wang Zheng , Zhong Yan , Wang Xiangting 
Published: May 2023

Abstract
Objective. Evaluate the effectiveness of extracellular vesicles derived from mesenchymal stem cells (MSCs) in the treatment of chronic kidney disease based on meta-analysis.
Methods. We searched CNKI, Wanfang, VIP, SinoMed, PubMed, Cochrane Library, EMbase, Web of Science and other databases on randomized controlled trial (RCT) of oral traditional Chinese patent medicines and simple preparations to improve inflammatory response in patients with diabetes nephropathy. The search time was from the establishment of the database to October 2022. All researchers independently screened and extracted documents for quality evaluation. Data analysis was conducted on documents that met quality standards using Stata 16.0 software and RevMan 5.4.
Results. 12 studies (n=198) satisfied the inclusion criteria.The results demonstrated that the levels of Scr (SMD=-0.38;95%CI=-4.29,-1.87;P<0.00001),BUN (SMD =−3.68, 95%CI=−5.24, −2.13;P<0.00001),and COL-1(SMD=-5.14; 95%CI=-9.32, -0.97; P<0.00001); α-sma SMD=-4.95%, 95%CI=-5.49,-2.50;P<0.00001); TGF-β (SMD=-4.19%, 95%CI=-6.92, -1.46;P=0.003) and the apoptotic cells (SMD=-3.85%, 95%CI=-5.05, -2.65;P＜0.00001) were significantly decreased in the EV group.
Conclusion. The results confirmed that MSCs-EV therapy could improve renal function and delaying kidney fibrosis in preclinical animal CKD model.
Influence of external insert Angle on structural deformation of long pipe shed with shallow hole
Authors: Jiang Chao teng , Mao Wei , Zhang Yongkang , Liu Xuejun , Muhemaier Ruheiyan , Xie Liangfu 
Published: May 2023

Abstract
The long pipe shed advanced support has the characteristics of large construction difficulty, wide support range, and easy deviation. The difference in construction dip angle will produce a different plastic zone of surrounding rock and supporting effects, and the rationality of advanced support design plays an important role in the safety of tunnel opening excavation. Based on the Tianshan Tunnel project, this paper aims at the problems of the loose pebble layer structure, poor cohesion, strong permeability, shallow excavation is not easy to form a confined arch, and easy to causes surrounding rock deformation and failure. Combined with the new method theory, the finite difference software FLAC3D was used to simulate seven excavation schemes of the shallow tunnel entrances. The mechanism and effect of advance support and the influence of construction angle on support effect are analyzed, and the simulation values are compared with the measured data. The results show that using long pipe shed advanced support can effectively reduce the disturbance of excavation to lose pebble rock mass and reduce the convergence value of surrounding rock. The results show that using long pipe shed advanced support can effectively reduce the disturbance of excavation to lose pebble rock mass and reduce the convergence value of surrounding rock. The plastic zone of surrounding rock produced by different angles can be divided into three parts. The structural stability difference of the C1 zone is small, and that of the C3 zone is large. The bending moment, shear force, and shape variables of the pipe shed decrease with the increase of the dip Angle, while the axial force increases with the increase of the dip Angle. According to the fitting effect of simulation results and measured values, the best supporting effect is when the dip Angle of shallow-buried tunnel advance support pipe shed is set in the range of 0°~3°.