bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peranan Badan Wakaf Indonesia Dalam Menangani Sengketa Wakaf Di Indonesia
Authors: Nabila Veren Estefany  , Nur Iza Faizah  , Fitri Nur Latifah  
Published: March 2023

Wakaf merupakan satu sistem Islam yang erat kaitannya dengan kesejahteraan umat dan melembaga sejak lama. Wakaf di masyarakat tidak hanya dalam pelaksanaan wakaf, tetapi juga mengelola tanah wakaf agar sesuai dengan prinsip Syariah. Wakaf adalah kegiatan muamalah dengan aspek spiritual, sosial dan ekonomi. Salah satu tujuan dibentuknya Badan Wakaf Indonesia (BWI) adalah untuk memajukan dan mengembangkan Wakaf nasional, dan Badan Wakaf Indonesia berperan aktif. Penelitian ini menggunakan pendekatan tinjauan pustaka dengan mengumpulkan data dari buku, artikel, atau jurnal. Hasil penelitian adalah peran Badan Wakaf Indonesia (BWI) dapat menyelesaikan sengketa Wakaf dengan baik dan mengamankan aset Wakaf selain menjadi Nadzir, untuk dapat berperan sebagai pembina Nadzir. BWI dapat mengelola dan berkembang secara produktif sehingga menghasilkan wakaf yang dapat mensejahterahkan umat. Dalam penyelesaiannya sengketa wakaf dapat juga menggunakan musyawarah dan bisa dibantu oleh pihak ketiga seperti pengadilan agama.
PERAN DINAS PARIWISATA DAN EKONOMI KREATIF DALAM PENGEMBANGAN OBJEK WISATA PANTAI LASIANA DI KOTA KUPANG PROVINSI NUSA TENGGARA TIMUR
Authors: Mulyana Mulyana , Fanggi Daryl Christo 
Published: March 2023

Terjadinya tumpang tindih status kepemilikan lahan masyarakat atau swasta, dan kurangnya jumlah sarana dan prasarana serta fasilitas yang tersedia di Pantai Lasiana menjadi fokus permasalahan penelitian ini. Tujuan penelitian adalah untuk mengetahui gambaran umum peran Dinas Pariwisata dan Ekonomi Kreatif dalam pengembangan objek wisata Pantai Lasiana. Penelitian ini merupakan penelitian deskriptif kualitatif dimana peneliti melakukan wawancara kepada 13 orang informan, observasi dan dokumentasi serta melakukan analisis data dengan reduksi dan penyajian data, serta penarikan kesimpulan. Temuan penelitian menunjukkan bahwa kurangnya fasilitas yang disediakan oleh pemerintah daerah setempat baik dari aspek kuantitas maupun kualitas menjadi salah satu faktor pendorong minat kunjungan wisatawan. Disamping itu, penyebarluasan informasi yang dilakukan oleh pemerintah daerah setempat dinilai kurang efektif sehinggapemerintah daerah perlu memperhatikan kualitas informasi yang disajikan dalam melakukan promosi. Masyarakat lokal sudah diberikan pelatihan dan pembinaan serta sosialisasi dalam rangka peran pemerintah sebagai terapi pada pengembangan objek wisata Pantai Lasiana. Kesimpulan penelitian menunjukkan bahwa Dinas Pariwisata dan Ekonomi Kreatif di Kota Kupang telah menyelenggarakan peran sebagai kebijakan, strategi, alat komunikasi, alat penyelesaian sengketa dan terapi. Namun masih terdaat hambatan dalampelaksanaannya. Penulis menyarankannya agar pemerintah daerah dapat merumuskan kebijakan terkait, mengevaluasi kegiatan promosi yang telah dilakukan dan memperbaiki serta meningkatkan fasilitas objek wisata Pantai Lasiana.
Kata Kunci: peran, pemerintah daerah, objek wisata
PERAN SATUAN TUGAS COVID-19 DALAM PENANGANAN COVID-19 DI KOTA DUMAI PROVINSI RIAU
Authors: Rinanda Muhammad Alvin Naufal 
Published: March 2023

Covid-19 yang melanda dunia saat ini membutuhkan perhatian khusus dalam penanganan salah satunya adalah penerapan protokol kesehatan oleh satuan tugas covid-19. Melalui Keputusan Walikota Dumai Nomor 305/BPBD/2020 Tentang Pembentukan Gugus Tugas Percepatan Corona Virus Disease 2019 (Covid-19) di Kota Dumai merupakan langkah serius pemerintah dalam menangani virus covid-19. Tujuan penelitian ini untuk mengetahui peranan Satuan Tugas Covid-19 dalam penanganan covid-19 di Kota Dumai Provinsi Riau. Penelitian ini menggunakan metode penelitian deskriptif kualitatif dengan pendekatan induktif, dimana penulis memperoleh data dengan cara wawancara, observasi dan dokumentasi. Hasil penelitian menunjukkan bahwa peranan Satuan Tugas dalam penanganan covid-19 sudah berjalan dengan baik, karena terjadinya penurunan jumlah para pelanggar protokol kesehatan yang berakibat pula pada penurunan kasus covid-19. Namun, perlu adanya peningkatan terutama sosialisasi secara menyeluruh kepada masyarakat dengan komunikasi yang baik beserta pendekatan-pendekatan lainnya. Kesimpulan penelitian menunjukkan bahwa peranan Satuan Tugas dalam penanganan covid-19 sudah berjalan dengan baik, karena terjadinya penurunan jumlah para pelanggar protokol kesehatan yang berakibat pula pada penurunan kasus covid-19. Dalam pelaksanaannya terdapat hambatan yang dihadapi oleh satuan tugas covid-19 yaitu tidak adanya aturan baku terkait penegakan protokol kesehatan beserta sanksi sehingga diharapkan segera adanya aturan tersebut. Upaya yang dilaksanakan oleh satuan tugas covid-19 adalah mengajak masyarakat untuk lebih peduli terhadap protokol kesehatan dan segera membuat aturan- aturan yang berkaitan dengan penanganan covid- 19.
Kata kunci: Peranan, Penanganan, Covid-19, Hambatan.
Cross-link augmentation enhances CFR-PEEK short fixation in lumbar metastasis stabilization
Authors: Borrelli Simone , Putame Giovanni , Audenino Alberto L. , Bignardi Cristina , Ferro Andrea , Marone Stefano , Terzini Mara 
Published: March 2023

Introduction: Spinal stability plays a crucial role in the success of the surgical treatment of lumbar vertebral metastasis and, in current practice, less invasive approaches such as short constructs have been considered. Concurrently, carbon fiber-reinforced (CFR) poly-ether-ether-ketone (PEEK) fixation devices are expanding in oncologic spinal surgery thanks to their radiotransparency and valid mechanical properties. This study attempts to provide an exhaustive biomechanical comparison of different CFR-PEEK surgical stabilizations through a highly reproducible experimental setup.Methods: A Sawbones biomimetic phantom (T12-S1) was tested in flexion, extension, lateral bending, and axial rotation. An hemisome lesion on L3 vertebral body was mimicked and different pedicle screw posterior fixations were realized with implants from CarboFix Orthopedics Ltd: a long construct involving two spinal levels above and below the lesion, and a short construct involving only the levels adjacent to L3, with and without the addition of a transverse rod-rod cross-link; to provide additional insights on its long-term applicability, the event of a pedicle screw loosening was also accounted.Results: Short construct reduced the overloading onset caused by long stabilization. Particularly, the segmental motion contribution less deviated from the physiologic pattern and also the long-chain stiffness was reduced with respect to the prevalent long construct. The use of the cross-link enhanced the short stabilization by making it significantly stiffer in lateral bending and axial rotation, and by limiting mobiliza-tion in case of pedicle screw loosening.Discussion: The present study proved in vitro the biomechanical benefits of cross-link augmentation in short CFR-PEEK fixation, demonstrating it to be a potential alternative to standard long fixation in the surgical management of lumbar metastasis.
CRISPR/Cas9-mediated genome editing in vancomycin-producing strain Amycolatopsis keratiniphila
Authors: Hu Mengyi , Chen Shuo , Ni Yao , Wei Wei , Mao Wenwei , Ge Mei , Qian Xiuping 
Published: March 2023

Amycolatopsis is an important source of diverse valuable bioactive natural products. The CRISPR/Cas-mediated gene editing tool has been established in some Amycolatopsis species and has accomplished the deletion of single gene or two genes. The goal of this study was to develop a high-efficient CRISPR/Cas9-mediated genome editing system in vancomycin-producing strain A. keratiniphila HCCB10007 and enhance the production of vancomycin by deleting the large fragments of ECO-0501 BGC. By adopting the promoters of gapdhp and ermE*p which drove the expressions of scocas9 and sgRNA, respectively, the all-in-one editing plasmid by homology-directed repair (HDR) precisely deleted the single gene gtfD and inserted the gene eGFP with the efficiency of 100%. Furthermore, The CRISPR/Cas9-mediated editing system successfully deleted the large fragments of cds13-17 (7.7 kb), cds23 (12.7 kb) and cds22-23 (21.2 kb) in ECO-0501 biosynthetic gene cluster (BGC) with high efficiencies of 81%–97% by selecting the sgRNAs with a suitable PAM sequence. Finally, a larger fragment of cds4-27 (87.5 kb) in ECO-0501 BGC was deleted by a dual-sgRNA strategy. The deletion of the ECO-0501 BGCs revealed a noticeable improvement of vancomycin production, and the mutants, which were deleted the ECO-0501 BGCs of cds13-17, cds22-23 and cds4-27, all achieved a 30%–40% increase in vancomycin yield. Therefore, the successful construction of the CRISPR/Cas9-mediated genome editing system and its application in large fragment deletion in A. keratiniphila HCCB10007 might provide a powerful tool for other Amycolatopsis species.
Novel bionic inspired nanosystem construction for precise delivery of mRNA
Authors: Yang Taihua , Xia Lei , Li Gen , Zhao Jie , Li Jie , Ge Jiahao , Yuan Qinggong , Zhang Jianjun , He Kang , Xia Qiang 
Published: March 2023

The intracellular delivery of messenger (m)RNA holds great potential for the discovery and development of vaccines and therapeutics. Yet, in many applications, a major obstacle to clinical translation of mRNA therapy is the lack of efficient strategy to precisely deliver RNA sequence to liver tissues and cells. In this study, we synthesized virus-like mesoporous silica (V-SiO2) nanoparticles for effectively deliver the therapeutic RNA. Then, the cationic polymer polyethylenimine (PEI) was included for the further silica surface modification (V-SiO2-P). Negatively charged mRNA motifs were successfully linked on the surface of V-SiO2 through electrostatic interactions with PEI (m@V-SiO2-P). Finally, the supported lipid bilayer (LB) was completely wrapped on the bionic inspired surface of the nanoparticles (m@V-SiO2-P/LB). Importantly, we found that, compared with traditional liposomes with mRNA loading (m@LNPs), the V-SiO2-P/LB bionic-like morphology effectively enhanced mRNA delivery effect to hepatocytes both in vitro and in vivo, and PEI modification concurrently promoted mRNA binding and intracellular lysosomal escape. Furthermore, m@V-SiO2-P increased the blood circulation time (t1/2 = 7 h) to be much longer than that of the m@LNPs (4.2 h). Understanding intracellular delivery mediated by the V-SiO2-P/LB nanosystem will inspire the next-generation of highly efficient and effective mRNA therapies. In addition, the nanosystem can also be applied to the oral cavity, forehead, face and other orthotopic injections.
Integration of molecular modelling and in vitro studies to inhibit LexA proteolysis
Authors: Schuurs Zachariah P. , McDonald John P. , Croft Laura V. , Richard Derek J. , Woodgate Roger , Gandhi Neha S. 
Published: March 2023

IntroductionAs antibiotic resistance has become more prevalent, the social and economic impacts are increasingly pressing. Indeed, bacteria have developed the SOS response which facilitates the evolution of resistance under genotoxic stress. The transcriptional repressor, LexA, plays a key role in this response. Mutation of LexA to a non-cleavable form that prevents the induction of the SOS response sensitizes bacteria to antibiotics. Achieving the same inhibition of proteolysis with small molecules also increases antibiotic susceptibility and reduces drug resistance acquisition. The availability of multiple LexA crystal structures, and the unique Ser-119 and Lys-156 catalytic dyad in the protein enables the rational design of inhibitors.MethodsWe pursued a binary approach to inhibit proteolysis; we first investigated β-turn mimetics, and in the second approach we tested covalent warheads targeting the Ser-119 residue. We found that the cleavage site region (CSR) of the LexA protein is a classical Type II β-turn, and that published 1,2,3-triazole compounds mimic the β-turn. Generic covalent molecule libraries and a β-turn mimetic library were docked to the LexA C-terminal domain using molecular modelling methods in FlexX and CovDock respectively. The 133 highest-scoring molecules were screened for their ability to inhibit LexA cleavage under alkaline conditions. The top molecules were then tested using a RecA-mediated cleavage assay.ResultsThe β-turn library screen did not produce any hit compounds that inhibited RecA-mediated cleavage. The covalent screen discovered an electrophilic serine warhead that can inhibit LexA proteolysis, reacting with Ser-119 via a nitrile moiety. DiscussionThis research presents a starting point for hit-to-lead optimisation, which could lead to inhibition of the SOS response and prevent the acquisition of antibiotic resistance.
Prognostic significance of neutrophil count on in-hospital mortality in patients with acute type A aortic dissection
Authors: Feng Weiqi , Li Huili , Wang Qiuji , Li Chenxi , Wu Jinlin , Yang Jue , Fan Ruixin 
Published: March 2023

BackgroundsThe goal of this study was to assess the impact of neutrophil count, in patients with acute type A aortic dissection (ATAAD).MethodsThis study retrospectively collected data from patients between September 2017 and June 2021. Youden's index was used to determine the optimal cut-off value for the neutrophil count and patients were divided into two subgroups. A restricted cubic spline (RCS) was used to model the relationship between variables and in-hospital mortality. The least absolute shrinkage and selection operator (LASSO) method and multivariate logistic regression analyses were used to investigate the independent prognostic factors for in-hospital mortality in patients with ATAAD.ResultsA total of 467 patients were enrolled in this study. In-hospital mortality was 7.28%. The group with elevated neutrophil counts had significantly higher mortality than the group with decreased neutrophil counts (10.8% vs. 3.2%, P = 0.02). This data shows that elevated neutrophil count was significantly associated with in-hospital mortality (OR 3.07, 95% CI 1.22–7.62, P = 0.02).ConclusionsNeutrophil count is an independent risk factor for in-hospital mortality in patients with ATAAD. It is an effective inflammatory index, which can be individualized for patients.
Contrast-enhanced ultrasound combined targeted microbubbles for diagnosis of highly aggressive papillary thyroid carcinoma
Authors: Ma Jiaojiao , Wang Yuan , Xi Xuehua , Tang Jiajia , Wang Linping , Wang Liangkai , Wang Di , Liang Xiaolong , Zhang Bo 
Published: March 2023

BackgroundAccurate diagnosis of highly aggressive papillary thyroid cancer (PTC) may greatly help avoid overdiagnosis and overtreatment of PTC. However, there is still a lack of a convenient and accurate method. Targeted microbubbles, an emerging ultrasound contrast agent, have the potential to accurately diagnose highly aggressive PTC.PurposeTo design and prepare a targeted microbubble for specific contrast-enhanced ultrasound (CEUS) imaging of highly invasive PTC.MethodsUsing β-galactoside-binding protein galectin-3 (Gal-3) overexpressed on the surface of highly invasive PTC cells as a target, C12 polypeptide (ANTPCGPYTHDCPVKR) with high affinity and specificity for Gal-3 was coupled to the surface of lipid microbubbles to prepare targeted microbubbles (Gal-3-C12@lipo MBs). The targeted microbubbles were prepared by thin-film hydration method and mechanical shaking method. The morphology, diameter, concentration and stability of microbubbles were investigated by fluorescence microscopy and an AccuSizer. The biosafety of microbubbles was studied using BCPAP cells through CCK8 assay. Confocal laser scanning microscope and flow cytometry were applied to research the cellular uptake of microbubbles to investigate the targeting ability to highly aggressive PTC. Finally, the specific contrast-enhanced ultrasound imaging of microbubbles in highly invasive PTC was validated on the mice bearing subcutaneous BCPAP tumor model via a clinically ultrasound imaging system.ResultsGal-3-C12@lipo MBs were successfully prepared which showed a well-defined spherical morphology with an average diameter of 1.598 ± 0.848 μm. Gal-3-C12@lipo MBs showed good stability without rupture within 4 hours after preparation. At the cellular level, Gal-3-C12@lipo MBs exhibited favorable biosafety and superior targeting ability to BCPAP cells, with 2.8-fold higher cellular uptake than non-targeted lipid microbubbles (Lipo MBs). At the animal level, Gal-3-C12@lipo MBs significantly improved the quality of contrast-enhanced ultrasound imaging in highly invasive PTC, with an echo intensity of tumor significantly higher than that of Lipo MBs.ConclusionWe designed and fabricated a novel targeted microbubble for the specific ultrasound imaging diagnosis of highly aggressive PTC. The targeted microbubbles have good stability, superior biosafety and high targeting specificity, which can significantly improve the tumor signal-to-noise ratio of highly invasive PTC, and have the potential to facilitate and accurately diagnose highly invasive PTC.
Trends and outcomes of fertility preservation for girls, adolescents and young adults with Turner syndrome: A prospective cohort study
Authors: Rodriguez-Wallberg Kenny A. , Sergouniotis Fotios , Nilsson Hanna P. , Lundberg Frida E. 
Published: March 2023

BackgroundIn Scandinavian countries, programs for fertility preservation (FP) are offered free of charge at tertiary-care university hospitals to all patients facing infertility risks due to malignant diagnoses or benign conditions. In this prospective study we aimed to investigate trends and outcomes of FP indicated by a diagnosis of Turner syndrome.MethodsProspective cohort study of patients with Turner karyotype receiving fertility preservation counselling at the Karolinska University Hospital between 1 January 1999 and 31 December 2021.ResultsThe cohort included 100 women and girls that received counselling, whereof 27% were prepubertal girls, 59% were adolescents and 14% of adult age. Before 2006 all patients were referred for fertility counselling at the time of Turner diagnosis. Based on updated guidelines, mainly patients who showed signs of puberty were referred after 2006. As a result, spontaneous menarche was more common in the later period. In total, 39% of the cohort had monosomal karyotype (45X), 20% had 45X/46XX or 45X/47XXX mosaicisms and 36% had an X-chromosomal structural anomaly. Ovarian tissue cryopreservation was planned for 73% of all patients, and oocyte cryopreservation following gonadotropin stimulation was planned for 10% of the patients. Follicles were present in 25% of all biopsies analyzed. Adolescents were more likely to have follicles present (30%) than prepubertal girls (16%) or adult women (17%). The ten patients that underwent gonadotropin stimulation for oocyte cryopreservation underwent a total of 15 cycles and eight patients successfully preserved oocytes. In total, 26% of the cohort has undergone fertility treatment or expressed further interest in fertility preservation. Six women have given birth using donated oocytes and three following spontaneous conception. Two women have undergone re-transplantation of cryopreserved ovarian tissue, without regaining ovarian function, and none of the women that have cryopreserved oocytes has returned to use them.ConclusionFertility counselling for girls with Turner syndrome should ideally be offered at onset of spontaneous puberty to improve the chances of fertility preservation. Since the girls and women in this cohort are still young, the return rate and utilization of the preserved tissue and oocytes is expected to increase with time.Clinical Trial RegistrationClinicalTrials.gov, identifier NTC04602962.
Genome-wide analyses of lung cancer after single high-dose radiation at five time points (2, 6, 12, 24, and 48 h)
Authors: Du Yajing , Zheng Yunna , Yu Kaiwen , Zhan Cheng , Qiao Tiankui 
Published: March 2023

Background: An increasing number of clinicians are experimenting with high-dose radiation. This study focuses on the genomic effects of high-dose single-shot radiotherapy and aims to provide a dynamic map for non-small cell lung cancer (NSCLC).Methods: We used whole-transcriptome sequencing to understand the evolution at molecular levels in A549 and H1299 exposed to 10 Gy X-rays at different times (2, 6, 12, 24, and 48 h) in comparison with the no radiation group. Ingenuity pathway analysis, ceRNA analysis, enrichment analysis, and cell cycle experiments are performed for molecular analyses and function analyses.Results: Whole-transcriptome sequencing of NSCLC showed a significant dynamic change after radiotherapy within 48 h. MiR-219-1-3p and miR-221-3p, miR-503-5p, hsa-miR-455-5p, hsa-miR-29-3p, and hsa-miR-339-5p were in the core of the ceRNA related to time change. GO and KEGG analyses of the top 30 mRNA included DNA repair, autophagy, apoptosis, and ferroptosis pathways. Regulation of the cell cycle-related transcription factor E2F1 might have a key role in the early stage of radiotherapy (2.6 h) and in the later stage of autophagy (24 and 48 h). Functions involving different genes/proteins over multiple periods implied a dose of 10 Gy was related to the kidney and liver pathway. Radiation-induced cell cycle arrest at the G2/M phase was evident at 24 h. We also observed the increased expression of CCNB1 at 24 h in PCR and WB experiments.Conclusion: Our transcriptomic and experimental analyses showed a dynamic change after radiation therapy in 48 h and highlighted the key molecules and pathways in NSCLC after high-dose single-shot radiotherapy.
A systematic review and meta-analysis of the effectiveness and safety of COVID-19 vaccination in older adults
Authors: Xu Kun , Wang Zihan , Qin Maorong , Gao Yangyu , Luo Na , Xie Wanting , Zou Yihan , Wang Jie , Ma Xingming 
Published: March 2023

In the coronavirus disease 2019 (COVID-19) pandemic, vaccinations were essential in preventing COVID-19 infections and related mortality in older adults. The objectives of this study were to evaluate the effectiveness and safety of the COVID-19 vaccines in older adults. We systematically searched the electronic bibliographic databases of PubMed, Web of Science, Embase, Cochrane Library, ClinicalTrials.gov, Research Square, and OpenGrey, as well as other sources of gray literature, for studies published between January 1, 2020, and October 1, 2022. We retrieved 22 randomized controlled trials (RCTs), with a total of 3,404,696 older adults (aged over 60 years) participating, that were included in the meta-analysis. No significant publication bias was found. In the cumulative meta-analysis, we found that the COVID-19 vaccines were effective in preventing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (OR = 0.38, 95% CI = 0.23–0.65, p = 0.0004) and in reducing the number of COVID-19-related deaths (OR = 0.16, 95% CI = 0.10–0.25, p &lt; 0.00001) in elderly people. Antibody seroconversion (AS) and geometric mean titer (GMT) levels significantly increased in vaccinated older adults [OR = 24.42, 95% CI = 19.29–30.92; standardized mean difference (SMD) = 0.92, 95% CI = 0.64–1.20, respectively]. However, local and systemic adverse events after COVID-19 vaccine administration were found in older adults (OR = 2.57, 95% CI = 1.83–3.62, p &lt; 0.00001). Although vaccination might induce certain adverse reactions in the elderly population, the available evidence showed that the COVID-19 vaccines are effective and tolerated, as shown by the decrease in COVID-19-related deaths in older adults. It needs to be made abundantly clear to elderly people that the advantages of vaccination far outweigh any potential risks. Therefore, COVID-19 vaccination should be considered as the recommended strategy for the control of this disease by preventing SARS-CoV-2 infection and related deaths in older adults. More RCTs are needed to increase the certainty of the evidence and to verify our conclusions.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022319698, identifier CRD42022319698.