bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of a Selective Liver Lobe Tumor-Bearing Mouse Model of Colorectal Cancer
Authors: Han Zheqi , Qiu Biying , Li Lin , Yu Jianhua , Zhu Zhiyang 
Published: August 2023

Purpose Colorectal cancer is one of the most common causes of cancer-related death, and its main site of metastasis is the liver. The surgical method used for metastases of colorectal cancer in the liver varies according to the lobe affected, as does the prognosis. However, there is a lack of relevant basic research. Therefore, a good animal model is needed for basic studies of metastases from colorectal cancer to the different lobes of the liver. Methods A CT26 colon cancer cell line transfected with a virus expressing green fluorescent protein was inoculated into BALB/C mice via the spleen. Tumor formation in the liver lobes was observed under a fluorescence microscope according to which portal vein branch was ligated and according to clamping time. The differential formation of metastatic lesions in the different lobes was then compared with physical anatomy. Serum samples were used to detect the changes in liver function postoperatively. Results Ligation and resection of the spleen 1 min after injection of the CT26 cells and release of the vessel clamp 1 min after splenectomy created an ideal tumor-bearing mouse model with little effect on liver function. Selective clamping of each portal vein branch and splenic injection of a CT26 cell line successfully established a selective liver lobe tumor-bearing model of colorectal cancer with distinct characteristics. Conclusion This model provides an opportunity for investigation of the mechanisms of metastasis of colorectal cancer to different lobes of the liver and may provide a basis for clinical treatment.
Valorization of Baker Yeast Industry Waste in Agriculture by Improving Germination and Growth of Barley and Pea
Authors: Aissani Nadhem , Aissani Rania , Ghidaoui Makrem , Zouidi Ferjeni , Sebai Hichem 
Published: August 2023

Industrial waste still present an environmental danger for the nature and survival of all living beings. Among these toxic products, the focus has been on liquid effluents from the baker's yeast industry that cause real environmental problems mainly due to their pollutant load and the release of unpleasant odors. In order to minimize these hazards and to take advantage of these wastes for the sake of our environment, the present work consists on valorizing effluents from the baker's yeast industry on barley ( Hordeum vulgare) and pea ( Pisum sativum), two important agricultural products of Tunisian north-west. Results showed that this waste is characterized by its richness in organic matter, and the presence of proteins traces with high chemical and biochemical oxygen demand (COD and BOD5) values. Diluted effluent at a dose of 2.5 mg/g significantly improves germination of both plant seeds by germination index (GI) calculation, to reach a maximum of 190 ± 17% and 150 ± 14% for barley and pea, respectively. In fertigation experiment, the use of a lower dose of .62 mg/g of diluted effluent promotes plant length to reach 52 ± 4 cm and 45 ± 1.4 cm, respectively, for H. vulgare and P. sativum. Gas chromatography coupled to mass spectrometry (GC-MS) analysis after derivatization showed significant enhancement of auxin production in pea treated with .62 mg/g of cream compared to control with a concentration of 10.60 ± .81 and 8.16 ± .43 ng/gFW, respectively. In another experiment, the irrigation of pea plants with furfural, as major compound of cream, promotes length and auxin production to reach 9.89 ± .56 ng/gFW for a furfural dose of .31 mg/g. This leads us to valorize baker’s yeast effluent as an environment-friendly natural product in pea and barley agricultural and give insight to its mode of action.
The balloon occlusion sheath for stroke (BOSS) balloon guide catheter for stroke intervention: Safety and technical success
Authors: Cuoco Joshua A , Entwistle John J , Siddiq Farhan , Puri Ajit S , Woodward Keith , Hanel Ricardo A , Ansari Sameer A , Frei Donald , Patel Biraj M 
Published: August 2023

Background We describe the first-in-human experience using the Balloon Occlusion Stroke Sheath (BOSSTM) balloon-guide catheter to perform stroke thrombectomy in 50 consecutive patients enrolled in the Flow Arrest Safety and Technical success with balloon-guide catheters trial. This aspiration system includes a novel 9.4F balloon-guide catheter conduit for the insertion and guidance of catheters with a balloon providing temporary flow arrest. Methods The Flow Arrest Safety and Technical success with balloon-guide catheter trial is a single-arm, prospective, multi-center, non-randomized, observational registry evaluating the use of the market-released BOSSTM balloon-guide catheter in adult patients diagnosed with an acute ischemic stroke attributable to large vessel occlusion. The purpose of the current trial was to assess the safety and technical success associated with the use of the BOSSTM balloon-guide catheter. Results Fifty patients met inclusion criteria with a mean baseline National Institutes of Health Stroke Scale (NIHSS) of 16. Treatment devices, including aspiration and stent retriever devices, were used in a total of 88 passes. The BOSSTM balloon-guide catheter was compatible with all stroke thrombectomy treatment devices used in 98% (49/50) of procedures. Balloon inflation and flow arrest were achieved in 100% (50/50) and 98% (49/50) of cases, respectively. Balloon deflation and retraction were observed in 100% (50/50) of cases. Successful reperfusion (modified thrombolysis in cerebral infarction score > 2b) was achieved in 100% of cases with single-pass reperfusion achieved in 62% (31/50) of cases. Conclusions The BOSSTM balloon-guide catheter is a safe and technically effective adjunctive device for mechanical thrombectomy of acute ischemic stroke due to large vessel occlusion.
Lidocaine for headache prevention during chronic subdural hematoma embolization
Authors: Diana Francesco , Romoli Michele , Ricchetti Francesca , Milonia Luca , Salcuni Andrea , Cirelli Carlo , Ruzza Alberto Di , Gaudino Chiara , Iacobucci Marta , Biraschi Francesco 
Published: August 2023

Background Middle meningeal artery embolization (MMAE) for the management of chronic subdural hematomas (CSDH) with ethylene vinyl alcohol (EVOH) causes an evident patient discomfort due to meningeal nociceptors stimulation. The aim of this study was to assess safety and efficacy of intra-arterial lidocaine (IAL) before MMAE of CSDH with EVOH. Methods We analyzed all consecutive patients with bilateral CSDH undergoing MMAE with EVOH. We used a monolateral IAL injection, with casual allocation. We assessed the headache felt by patients during embolization with the visual analog scale (VAS) and compared scores obtained after embolization of both sides. We followed the STROBE guidelines for case–control studies. Paired t-test and χ2 test were used to compare the distribution of variables in IAL vs control group. Results Between September 2021 and March 2023, 32 patients underwent bilateral MMAE with EVOH for a CSDH. Lidocaine treatment resulted in a substantially lower VAS score compared to the control group (median 3 vs 7, p < 0.001), with no substantial side effect. Compliance also benefited from lidocaine administration. Conclusions In patients with CSDH undergoing MMAE, IAL seems to reduce pain sensation associated with EVOH injection and to increase patients’ compliance during treatment.
Planning to conceive within a year is associated with better pregnancy-specific disease-related patient knowledge and better medication adherence in women of childbearing age with inflammatory bowel disease
Authors: Selinger Christian P. , Laube Robyn , Steed Helen , Brookes Matthew , BioResource NIHR , Leong Rupert W. L. 
Published: August 2023

Background: Adherence to inflammatory bowel disease (IBD) medication is crucial to maintain remission, especially during pregnancy. Objective: To examine the influence of family planning and pregnancy-related patient knowledge regarding IBD and pregnancy on adherence. Design: Cross-sectional survey study Methods: We surveyed female patients with IBD aged 18–35 years, who at recruitment to the UK IBD BioResource had not had children. We elicited disease and treatment history, demographics and family planning status via an online questionnaire . Patient knowledge as assessed by the validated Crohn’s and Colitis Pregnancy Knowledge Score (CCPKnow) and adherence by visual analogue scale (VAS). Results: In 326 responders (13.8% response rate), good adherence (VAS ⩾ 80) was found in only 38.35%. Disease- and treatment-related factors were not significantly associated with good adherence, except for methotrexate (70.0% adherent of 10 exposed patients versus 37.2% non-exposed; p = 0.036). Patients planning pregnancy for the next year were more often adherent (59.0% versus 35.5%; p = 0.019) and knowledgeable (median CCPKnow 8 versus 7; p = 0.035) compared to those in other family planning categories. Pregnancy-related patient knowledge was significantly associated with adherence (Pearson correlation 0.141; p = 0.015). Adherent patients had significantly higher CCPKnow scores than non-adherent patients (median 8 versus 6; p = 0.009). On binary regression analysis, only planning to conceive within 12 months was independently associated with better adherence ( p = 0.016), but not methotrexate exposure ( p = 0.076) and CCPKnow ( p = 0.056). Conclusions: In a cohort of women of childbearing age with IBD overall medication, adherence was low. Planning to conceive within the next year was associated with better adherence and greater patient knowledge.
Impact of the coronavirus disease 2019 pandemic on sarcoma management in France: a 2019 and 2020 comparison
Authors: Penel Nicolas , Cantarel Coralie , Chemin-Airiau Claire , Ducimetiere Françoise , Gouin François , Le Loarer François , Toulmonde Maud , Piperno-Neumann Sophie , Bellera Carine , Honore Charles , Blay Jean-Yves , Mathoulin-Pelissier Simone 
Published: August 2023

Background: The coronavirus disease 2019 (COVID-19) pandemic was an unprecedented shock to the healthcare systems, and its consequences on managing rare cancers are unknown. We investigated COVID-19’s impact on the activity of sarcoma-labeled networks by comparing key indicators in 2019–2020 (before and during the pandemic, respectively). Methods: We compared the incidence of limb and trunk soft tissue sarcomas, surgery rate, surgery center, surgery quality, and surgery delays nationally and in various regions, focusing on the three most severely affected regions. Findings: In this study, sarcoma incidence did not decrease, and the tumor and patient characteristics were similar in both years. The number of patients who underwent surgery in the labeled centers increased significantly (63% versus 57%, p = 0.015), the rate of R0 resection increased (55% versus 47%, p = 0.004), and the rate of re-excision decreased (12% versus 21%, p < 0.0001). In the univariate analysis, the time to surgery was similar in both years. Cox regression analysis revealed that the factors associated with a longer time to surgery were age > 70 years ( p = 0.003), retroperitoneal location ( p > 0.001), tumor size ( p < 0.001), deep tumors ( p < 0.001), and regions ( p < 0.001). However, we have observed an increase in the time before surgery in the regions most stroked by the COVID-19 pandemic. Interpretation: The model of the labeled center network for managing rare tumors was resilient. Paradoxically, the quality indicators improved during the pandemic due to the direct referral of patients with sarcomas to the labeled centers. Summary: This study shows that a nationwide network organization has made it possible to maintain care for these rare tumors during the pandemic.
Il-1β Promotes Superficial Zone Cells Senescence in Articular Cartilage by Inhibiting Autophagy
Authors: Xu Wei , Wang Juan , Cui Lin , Huang Chen , Xia Ning , Xie Meiming , Liu Da , Liao Dongfa 
Published: August 2023

Objective The superficial zone cells in articular cartilage (SFZCs) have been identified as stem/progenitor chondrocytes and promoted cell self-renewal in the osteoarthritis (OA). Several studies emphasized the involvement of senescence and autophagy in OA. Interleukin-1β (IL-1β) is one of the main inflammatory mediators of OA, and whether it induces senescence and autophagy in SFZCs remains unclear. The present study aimed to investigate autophagy flux, mitochondrial function, and intracellular reactive oxygen species (ROS) that resulted in senescence in SFZCs induced by IL-1β. Methods Using western blotting, reverse transcription-quantitative PCR, immunofluorescence, intracellular ROS detection, mitochondrial staining, and determination of mitochondrial membrane potential, we tested senescence and autophagy markers in SFZCs induced by IL-1β in vitro. The consequences of mitochondrial function and ROS were also studied with IL-1β-induced senescence. Results IL-1β treatment decreased SFZC proliferation, induced SFZC senescence, and reduced SFZCs’ chondrogenic differentiation capacity. Moreover, IL-1β impaired autophagy flux, and the autophagy activator, rapamycin, attenuated the senescence of SFZCs. IL-1β-induced autophagy defect resulted in mitochondrial dysfunction and overproduction of ROS, and autophagy activation notably protected against mitochondrial dysfunction and reduced the levels of ROS. Moreover, antioxidant N-acetylcysteine reversed the senescence of IL-1β in SFZCs. Conclusion IL-1β promotes autophagy impairment and subsequently results in dysfunctional mitochondria and overproduction of ROS, which finally causes SFZC senescence.
Understanding the impact of digital technology on the well-being of older immigrants and refugees: A scoping review
Authors: Ekoh Prince Chiagozie , Okolie Tochukwu Jonathan , Nnadi Fidel Bethel , Oyinlola Oluwagbemiga , Walsh Christine A 
Published: August 2023

Background The fast-paced development of digital technologies in the areas of social media, pet robots, smart homes, and artificial intelligence, among others, profoundly influence the daily lives of older adults. Digital technology can improve the well-being and quality of life of older adults, older immigrants and refugees who suffer migration-associated stress, loneliness, health and psychosocial challenges. Aims The aim of this scoping review is to map out extant empirical literature that has examined the implication of digital technology among older refugees and immigrants. Methods Using a 1 five-stage framework, we conducted a scoping review of peer-reviewed empirical studies published in English with no time restrictions. We searched nine databases for the reviews, and abstracts were reviewed using Rayyan QCRi(c) before the full-text review. The comprehensive database search yielded 4134 articles, of which 15 met the inclusion criteria. Results The results of the review suggest that digital technology is essential to the well-being, quality of life of older immigrants and refugees, especially for maintaining and building new social support networks, navigating opportunities, coping with migration-induced stress through e-leisure, and staying connected to their culture. The literature also revealed poor utilisation of digital technologies amongst older immigrants and refugees, suggesting barriers to access. Conclusion The study concluded by highlighting the need for more research and interventions that focus on multiple strategies, including education for increased access to and utilisation of digital technology to ensure that more older migrants can benefit from the advantages of digital technology in a safe way.
Diagnostic validation of smart wearable device embedded with single-lead electrocardiogram for arrhythmia detection
Authors: Niu Yonghong , Wang Hao , Wang Hong , Zhang Hui , Jin Zhigeng , Guo Yutao 
Published: August 2023

Objective To validate a single-lead electrocardiogram algorithm for identifying atrial fibrillation, atrial premature beats, ventricular premature beats, and sinus rhythm. Methods A total of 656 subjects aged 19 to 94 years were enrolled. Participants were simultaneously tested with a wristwatch (Huawei Watch GT2 Pro, Huawei Technologies Co., Ltd, Shenzhen, China) and a 12-lead electrocardiogram for 3 minutes. A total of 1926 electrocardiogram signals from 628 subjects (282 men and 346 women) aged 19 to 94 years (median 64 years) were analyzed using an algorithm. Results The numbers of subjects with atrial fibrillation, atrial premature beats, ventricular premature beats, and sinus rhythm were 129, 141, 107, and 251, respectively, and together they had a total of 1926 electrocardiogram signals. For the three-class classification system, the recall, precision, and F1 score were 97.6%, 96.5%, 97.0% for sinus rhythm; 96.7%, 96.9%, 96.8% for atrial fibrillation; and 92.8%, 94.2%, 93.5% for ectopic beats, respectively. The macro-F1 score of the three-class classification system was 95.8%. For the four-class classification system, the recall, precision, and F1 score were 97.6%, 96.5%, 97.0% for sinus rhythm; 96.7%, 96.9%, 96.8% for atrial fibrillation; 90.5%, 89.4%, 89.9% for atrial premature beats; and 86.1%, 89.6%, 87.8% for ventricular premature beats, respectively. The macro-F1 score of the four-class classification system was 92.9%. Conclusions The single-lead electrocardiogram algorithm embedded into smart wearables demonstrated good performance in detecting atrial fibrillation, atrial/ventricular premature beats, and sinus rhythm, and thus would facilitate atrial fibrillation screening and management.
Decision Making in Combined Hip Arthroscopy and Femoral Derotational Osteotomy Surgeries
Authors: Steiner Quinn , Zacharias Anthony J. , Goodspeed David C. , Spiker Andrea M. 
Published: August 2023

Background: Femoral version abnormalities can contribute to intra-articular hip pathology. Combined hip arthroscopy with femoral derotational osteotomy (FDRO) has been shown to successfully treat those with intra-articular hip pathology with excessive anteversion or retroversion of the femur. Indications: We describe the technique for combined hip arthroscopy and FDRO in patients with symptomatic intra-articular hip pathology in the setting of excessive anteversion or retroversion of the femur. Technique Description: Hip arthroscopy is performed using standard anterolateral, modified mid-anterior, and distal anterolateral accessory portals. The labrum is repaired using a narrow diameter suture. Femoroplasty is performed with utilization of fluoroscopic imaging to assess resection. Dynamic flexion is performed as a final check of adequacy of resection. Capsular closure is performed in all cases. After hip arthroscopy, the patient is repositioned on a radiolucent table. A piriformis start point is obtained with a guide pin followed by standard opening reaming and ball-tipped guidewire placement. A femoral osteotomy is made just proximal to the isthmus and made through a lateral approach to the femur. Two K-wires are placed distal and proximal to the osteotomy site. A goniometer is then used to measure rotation. A drill is used to perforate the cortex circumferentially at the osteotomy site. After standard reaming, a sagittal saw is then used to start the osteotomy cut followed by an osteotome. An intramedullary nail is inserted over a ball-tipped guidewire while rotational reduction is assessed from the foot of the operating table. Distal interlocking screws are placed, and the nail is backslapped to create compression at the osteotomy site prior to placing proximal interlocking screws. Results: Recent studies show improved hip outcome scores in patients who undergo concomitant hip arthroscopy and FDRO for symptomatic abnormal femoral version. Discussion/Conclusion: Identifying patients who would benefit from concomitant surgeries requires thorough preoperative evaluation. Correct identification and treatment of these patients leads to improved outcomes. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.
Who cares about migraine? Pathways and hurdles in the European region - access to care III
Authors: Vaghi Gloria , De Icco Roberto , Tassorelli Cristina , Goadsby Peter J. , Vicente-Herrero Teófila , de la Torre Elena Ruiz 
Published: August 2023

Abstract
Background
Migraine is a highly prevalent primary headache disorder and a leading cause of disability. Difficulties in access to care during diagnostic and therapeutic journey contribute to the disease burden. Several target-specific drugs have reached the market in the past four years and have modified the treatment paradigm in migraine. The aim of this study is to provide an updated snapshot of the pathways and hurdles to care for migraine in different European countries by directly asking patients.

Methods
In 2021 the European Migraine and Headache Alliance proposed a 39-item questionnaire that was administered online to an adult migraine population in European countries. Questions were focused on socio-demographic and migraine data, access to diagnosis and treatment, disease-related burden and the main channel for disease information.

Results
A total of 3169 questionnaires were returned from 10 European countries. Responders were predominantly females, age range 25–59 years, with a migraine history longer than 10 years in 82% of cases, and with at least 8 headache days per month in 57% of cases. Respondents reported limitations in social, working and personal life during both the ictal and interictal phase. The activities mostly impaired during the attacks were driving (55%), cooking or eating (42%), taking care of family/childcare (40%) and getting medicines at the pharmacy (40%). The most frequently reported unmet need was the long delay between the first visit and migraine diagnosis: 34% of respondents had to see ≥ 4 specialists before being correctly diagnosed, and between the diagnosis and treatment prescription: > 5 years in 40% of cases. The most relevant needs in terms of quality of life were the desire for a lower migraine frequency, an effective treatment and a greater involvement in society.

Conclusions
Data from the present survey point to the existence and persistence of multiple hurdles that result in significant limitations to access to care and to the patients’ social life. A close cooperation between decision makers, healthcare workers and patients is needed to overcome these barriers.

Graphical Abstract