bluuebunny/crossref_metadata_2025_split huggingface dataset:
Duodenal lipoma: robotic-assisted approach
Authors: Alghanemi Ryan , Tekin Zülküf 
Published: May 2023

Abstract
Lipomas are one of the most common benign tumors of the gastrointestinal tract, typically found in the small and large intestine. While most cases are asymptomatic and discovered incidentally, large duodenal lipomas are rare and present a unique set of diagnostic and management challenges due to their complex anatomic relationships with other vital structures. Endoscopic removal of large lipomas carries a risk of bleeding and can be difficult to access. To address these issues, robotic-assisted surgical approaches have been proposed as a viable alternative to laparoscopy, as demonstrated in this case.
Low Spontaneous Mutation Rate in Complex Multicellular Eukaryotes with a Haploid–Diploid Life Cycle
Authors: Krasovec Marc , Hoshino Masakazu , Zheng Min , Lipinska Agnieszka P , Coelho Susana M 
Published: May 2023

Abstract
The spontaneous mutation rate µ is a crucial parameter to understand evolution and biodiversity. Mutation rates are highly variable across species, suggesting that µ is susceptible to selection and drift and that species life cycle and life history may impact its evolution. In particular, asexual reproduction and haploid selection are expected to affect the mutation rate, but very little empirical data are available to test this expectation. Here, we sequence 30 genomes of a parent–offspring pedigree in the model brown alga Ectocarpus sp.7, and 137 genomes of an interspecific cross of the closely related brown alga Scytosiphon to have access to the spontaneous mutation rate of representative organisms of a complex multicellular eukaryotic lineage outside animals and plants, and to evaluate the potential impact of life cycle on the mutation rate. Brown algae alternate between a haploid and a diploid stage, both multicellular and free living, and utilize both sexual and asexual reproduction. They are, therefore, excellent models to empirically test expectations of the effect of asexual reproduction and haploid selection on mutation rate evolution. We estimate that Ectocarpus has a base substitution rate of µbs = 4.07 × 10−10 per site per generation, whereas the Scytosiphon interspecific cross had µbs = 1.22 × 10−9. Overall, our estimations suggest that these brown algae, despite being multicellular complex eukaryotes, have unusually low mutation rates. In Ectocarpus, effective population size (Ne) could not entirely explain the low µbs. We propose that the haploid–diploid life cycle, combined with extensive asexual reproduction, may be additional key drivers of the mutation rate in these organisms.
MSH2-MSH3 promotes DNA end resection during homologous recombination and blocks polymerase theta-mediated end-joining through interaction with SMARCAD1 and EXO1
Authors: Oh Jung-Min , Kang Yujin , Park Jumi , Sung Yubin , Kim Dayoung , Seo Yuri , Lee Eun A , Ra Jae Sun , Amarsanaa Enkhzul , Park Young-Un , Lee Seon Young , Hwang Jung Me , Kim Hongtae , Schärer Orlando , Cho Seung Woo , Lee Changwook , Takata Kei-ichi , Lee Ja Yil , Myung Kyungjae 
Published: May 2023

Abstract
DNA double-strand break (DSB) repair via homologous recombination is initiated by end resection. The extent of DNA end resection determines the choice of the DSB repair pathway. Nucleases for end resection have been extensively studied. However, it is still unclear how the potential DNA structures generated by the initial short resection by MRE11-RAD50-NBS1 are recognized and recruit proteins, such as EXO1, to DSB sites to facilitate long-range resection. We found that the MSH2-MSH3 mismatch repair complex is recruited to DSB sites through interaction with the chromatin remodeling protein SMARCAD1. MSH2-MSH3 facilitates the recruitment of EXO1 for long-range resection and enhances its enzymatic activity. MSH2-MSH3 also inhibits access of POLθ, which promotes polymerase theta-mediated end-joining (TMEJ). Collectively, we present a direct role of MSH2-MSH3 in the initial stages of DSB repair by promoting end resection and influencing the DSB repair pathway by favoring homologous recombination over TMEJ.
Core defense hotspots within Pseudomonas aeruginosa are a consistent and rich source of anti-phage defense systems
Authors: Johnson Matthew C , Laderman Eric , Huiting Erin , Zhang Chi , Davidson Alan , Bondy-Denomy Joseph 
Published: May 2023

Abstract
Bacteria use a diverse arsenal of anti-phage immune systems, including CRISPR-Cas and restriction enzymes. Recent advances in anti-phage system discovery and annotation tools have unearthed many unique systems, often encoded in horizontally transferred defense islands, which can be horizontally transferred. Here, we developed Hidden Markov Models (HMMs) for defense systems and queried microbial genomes on the NCBI database. Out of the 30 species with &gt;200 completely sequenced genomes, our analysis found Pseudomonas aeruginosa exhibits the greatest diversity of anti-phage systems, as measured by Shannon entropy. Using network analysis to identify the common neighbors of anti-phage systems, we identified two core defense hotspot loci (cDHS1 and cDHS2). cDHS1 is up to 224 kb (median: 26 kb) with varied arrangements of more than 30 distinct immune systems across isolates, while cDHS2 has 24 distinct systems (median: 6 kb). Both cDHS regions are occupied in a majority of P. aeruginosa isolates. Most cDHS genes are of unknown function potentially representing new anti-phage systems, which we validated by identifying a novel anti-phage system (Shango) commonly encoded in cDHS1. Identifying core genes flanking immune islands could simplify immune system discovery and may represent popular landing spots for diverse MGEs carrying anti-phage systems.
Proksee: in-depth characterization and visualization of bacterial genomes
Authors: Grant Jason R , Enns Eric , Marinier Eric , Mandal Arnab , Herman Emily K , Chen Chih-yu , Graham Morag , Van Domselaar Gary , Stothard Paul 
Published: May 2023

Abstract
Proksee (https://proksee.ca) provides users with a powerful, easy-to-use, and feature-rich system for assembling, annotating, analysing, and visualizing bacterial genomes. Proksee accepts Illumina sequence reads as compressed FASTQ files or pre-assembled contigs in raw, FASTA, or GenBank format. Alternatively, users can supply a GenBank accession or a previously generated Proksee map in JSON format. Proksee then performs assembly (for raw sequence data), generates a graphical map, and provides an interface for customizing the map and launching further analysis jobs. Notable features of Proksee include unique and informative assembly metrics provided via a custom reference database of assemblies; a deeply integrated high-performance genome browser for viewing and comparing analysis results at individual base resolution (developed specifically for Proksee); an ever-growing list of embedded analysis tools whose results can be seamlessly added to the map or searched and explored in other formats; and the option to export graphical maps, analysis results, and log files for data sharing and research reproducibility. All these features are provided via a carefully designed multi-server cloud-based system that can easily scale to meet user demand and that ensures the web server is robust and responsive.
Les recommandations en vigueur pour la prise en charge de l’acidocétose diabétique pédiatrique
Authors: Gripp Karen E , Trottier Evelyne D , Thakore Sidd , Sniderman Jonathan , Lawrence Sarah 
Published: May 2023

RÉSUMÉPour traiter l’acidocétose diabétique pédiatrique, il faut porter une attention particulière aux liquides et aux électrolytes pour limiter le risque de complications, telles qu’une lésion cérébrale, associée à une morbidité et une mortalité élevées. L’incidence d’œdème cérébral en cas d’acidocétose diabétique n’a pas diminué malgré les protocoles visant la limitation des liquides qui s’appuient sur la restriction de la réanimation liquidienne initiale. Selon de nouvelles données probantes, l’administration précoce de liquides isotoniques n’entraîne pas de risque supplémentaire et peut améliorer les résultats cliniques chez certains patients. Les protocoles et les directives cliniques sont adaptés et axés particulièrement sur la surveillance et le remplacement initiaux et continus des liquides et des électrolytes. Il est maintenant recommandé de commencer par une réanimation à l’aide de liquides isotoniques chez tous les patients dans les 20 à 30 minutes suivant leur arrivée à l’hôpital, suivie par la réplétion du déficit volumique sur une période de 36 heures, en association avec une perfusion d’insuline et des suppléments d’électrolytes, ainsi qu’avec la surveillance et la prise en charge attentives d’une éventuelle lésion cérébrale.
Relational Mechanisms to Explain Collective Performance Data Use
Authors: Kroll Alexander 
Published: May 2023

AbstractPerformance management theory has been largely organizational with a focus on the decision maker, operating within the public-sector hierarchy. But such an understanding misses most contexts that are more horizontal and fall somewhere between intra-organizational team structures and inter-organizational collaborations. To address this gap, this article puts forward the concept of collective performance data use; a group-level construct defined through the lateral, voluntary, and reciprocal negotiations among partners. Drawing on related literatures, it develops a theoretical framework to explain collective data use based on three relational mechanisms (system sensemaking, deliberation routines, and dissent-conflict balancing) and a set of mechanism-activating antecedents, out of which four are featured in greater detail: connectedness, power imbalance, expertise configurations, and distributed leadership. The article argues we need to update extant performance management theory using a relational perspective if we want to better understand the social side of performance practices and related behaviors.
Disturbance of sleep maintenance, but not sleep duration, activates nuclear factor-κB and signal transducer and activator of transcription family proteins in older adults: sex differences
Authors: Piber Dominique , Olmstead Richard , Cho Joshua H , Irwin Michael R 
Published: May 2023

Abstract

Study Objectives
Disturbances of sleep maintenance and sleep duration are common in older adults and associated with an increased risk for age-related mortality and morbidity. Converging evidence implicates inflammation as an underlying mechanism, especially in females. However, it is unknown what specific aspects of sleep disturbance impact inflammatory mechanisms in older adults.

Methods
Using data from community-dwelling older adults who participated in the Sleep Health and Aging Research (SHARE) field study (n = 262, mean age 71.9 ± 8.0 years), we conducted a secondary analysis to examine whether disturbance of sleep maintenance (i.e. greater amount of wake time after sleep onset [WASO]) and sleep duration (i.e. shorter total sleep time [TST]) assessed by sleep diary and actigraphy are associated with greater activation of nuclear factor (NF)-κB and signal transducer and activator of transcription (STAT) family proteins STAT1, STAT3, and STAT5 in peripheral blood monocytic cells. In addition, moderation effects of sex were explored.

Results
Data were available for sleep diary (n = 82), actigraphy (n = 74), and inflammatory signaling and transcriptional measures (n = 132). As assessed by sleep diary, greater amount of WASO (β = 0.39, p &lt; 0.01), but not TST, was associated with higher levels of NF-κB. Whereas diary-assessed sleep measures were not associated with STAT family proteins, a moderation analysis revealed that greater diary-assessed WASO was associated with higher levels of STAT1 (p &lt; 0.05), STAT3 (p &lt; 0.05), and STAT5 (p &lt; 0.01) in females, but not in males. Actigraphy-assessed sleep measures were not associated either with NF-κB or STAT activation.

Conclusions
In older adults, self-reported disturbance of sleep maintenance assessed by sleep diary was uniquely associated with higher levels of NF-κB, along with higher levels of STAT family proteins in females, but not in males. Our data suggest that improvingself-reported sleep maintenance might mitigate age-related increases in inflammatory signaling and transcriptional pathways, possibly more strongly in females, with the potential to reduce mortality risk in older adults.
A revision of the genus Porteria and the phylogeny and biogeography of Porteriinae (Araneae: Desidae)
Authors: Morrill Elizabeth , Crews Sarah , Esposito Lauren , Ramírez Martín J , Griswold Charles 
Published: May 2023

Abstract
We revise the Chilean genus Porteria, including the type species, Porteria albopunctata, and 11 new species: Porteria ajimayo sp. nov., Porteria alopobre sp. nov., Porteria ariasbohartae sp. nov., Porteria bunnyana sp. nov., Porteria contulmo sp. nov., Porteria correcaminos sp. nov., Porteria eddardstarki sp. nov., Porteria faberi sp. nov., Porteria fiura sp. nov., Porteria misbianka sp. nov. and Porteria torobayo sp. nov. A phylogenetic analysis using six genetic markers confirms the monophyly of Porteriinae, including Baiami and the core porteriines, here defined to include the ecribellate genera Cambridgea, Corasoides, Nanocambridgea and Porteria. Core porteriines are diagnosed by a narrowed section of the piriform gland spigot field, the cymbium extended to a narrow tip and lack of a median apophysis. Porteria and Corasoides are sister taxa, united by the behaviour of running atop the sheet of a web and by spinning a regular square mesh in the web platform. According to our results, the diversification of Porteria started about 30 Mya (44–17 Mya). A biogeographic analysis infers that an ancestor of Porteria reached South America via a founder event from Australia or New Zealand, where their close relatives occur.
Vaginal Corrosion Due to Insertion of a 9-Volt Battery
Authors: Moore Ellen , Friedman Julie , Christopher Diane 
Published: May 2023

BACKGROUND:
Batteries are known to cause damage to mucosal surfaces. Unfortunately, the timing of serious sequelae and recommendations for removal of a vaginally inserted battery in a premenopausal patient are not well characterized. This case report aims to detail the timeline of events and complications after vaginal insertion of a 9-volt alkaline battery and to further clarify the recommendation for urgent removal.

CASE:
A 24-year-old nulliparous woman with significant psychiatric and trauma history was admitted for ingestion and insertion of multiple foreign objects, including a 9-volt battery that she inserted into her vagina during her hospital admission. Examination under anesthesia was required for removal of the battery, with cervical and vaginal necrosis and partial-thickness burns noted. Removal occurred approximately 5.5 hours after insertion. Management included vaginal irrigation and topical estrogen.

CONCLUSION:
Given our findings of rapid and severe damage to the vaginal mucosa, urgent removal of a vaginally inserted battery is indicated.
Evolution of a Continuing Professional Development Program Based on a Community of Practice Model for Health Care Professionals in Resource-Limited Settings
Authors: Nguyen Diane , Tupas Kris Denzel , Thammasitboon Satid 
Published: May 2023

Introduction:
The Baylor International Pediatric AIDS Initiative (BIPAI) Network supports a network of independent nongovernmental organizations providing health care for children and families in low- and middle-income countries (LMIC). Using a community of practice (CoP) framework, a continuing professional development (CPD) program was created for health professionals to enhance knowledge and exchange best practices.

Methods:
An online learning platform (Moodle), videoconferencing (Zoom), instant messaging systems (Whatsapp), and email listserv facilitated learning and interaction between program participants. Target participants initially included pharmacy staff and expanded to include other health professionals. Learning modules included asynchronous assignments and review of materials, live discussion sessions, and module pretests and posttests. Evaluation included participants' activities, changes in knowledge, and assignment completion. Participants provided feedback on program quality via surveys and interviews.

Results:
Five of 11 participants in Year 1 earned a certificate of completion, and 17 of 45 participants earned a certificate in Year 2. Most modules showed an increase in module pretest and posttest scores. Ninety-seven percent of participants indicated that the relevance and usefulness of modules were good or outstanding. Ongoing evaluation indicated changes in Year 2 for program improvement, and notable outcomes indicated how CoP added value in developing a true community.

Discussion:
Using a CoP framework allowed participants to improve their personal knowledge and become part of a learning community and network of interdisciplinary health care professionals. Lessons learned included expanding program evaluation to capture potential value creation of the community of practice in addition to individual-level development; providing briefer, more focused programs to better serve busy working professionals; and optimizing use of technological platforms to improve participant engagement.
Mucinous adenocarcinoma of the urachus: a practical overview of a broad differential diagnosis: case report
Authors: Sánchez Germán J. , Guevara Raúl , Riscanevo Bobadilla Angie C. , Hernández Mayra A. , Duarte Ricardo , Balaguera Alberto 
Published: May 2023

Background:
Mucinous urachal neoplasms presenting with pseudomyxoma peritonei are rare pathologies, which usually manifest with abdominal pain, mucosuria, and imaging findings of an infraumbilical midline mass and bladder dome. Cytoreduction complemented with hyperthermic intraperitoneal chemotherapy (HIPEC) is a treatment strategy associated with better survival rates, disease-free time, and better quality of life compared with systemic chemotherapy.

Case Description:
We describe an unusual case of a patient with peritoneal pseudomyxoma secondary to mucinous adenocarcinoma of the urachus, who underwent complete macroscopic cytoreduction and intraoperative HIPEC with an adequate response to HIPEC, without imaging or clinical evidence of relapse at 24 months of follow-up.

Conclusion:
Cytoreductive surgery associated with HIPEC can be considered a treatment strategy for mucinous urachal neoplasms presenting with peritoneal pseudomyxoma.
Effectiveness of Therapeutic Community Program on Resilience and Change in Lifestyle in People With Alcohol Use Disorder
Authors: Song Eun Ae , Kim Hee Kyung , Lee Mihyoung 
Published: May 2023

Abstract
The purpose of this study was to examine the effectiveness of a therapeutic community program on resilience and positive change of lifestyle in people with alcohol use disorder. This study used a quasi-experimental study design. The Therapeutic Community Program was conducted daily for 12 weeks from June 2017 to May 2018. Subjects were selected from the Therapeutic Community and a hospital. Of the 38 subjects, 19 subjects belonged to the experimental group and 19 subjects belonged to the control group. Our findings were that the Therapeutic Community Program has improved resilience and promoted global lifestyle changes in the experimental group compared with the control group.
The Impact of Individual Counseling on Treatment for Opioid Use
Authors: Mariolis Tara , Wilson Amanda , Chiodo Lisa M. 
Published: May 2023

Abstract
In 2018, more than 67,000 people died because of drug overdoses, and of that number, approximately 69.5% involved an opioid, making it a leading cause of death in the United States. Additionally concerning is that 40 states have reported increased overdose deaths and opioid-related deaths since the start of the COVID-19 global pandemic. Presently, many insurance companies and healthcare providers require patients to receive counseling during medication treatment for opioid use disorder (OUD), despite the lack of evidence that it is necessary for all patients. To inform policy and improve quality of treatment, this nonexperimental, correlational study examined the relationship between individual counseling status and treatment outcomes in patients receiving medication treatment for OUD. Treatment outcome variables (treatment utilization, medication use, and opioid use) were extracted from the electronic health records of 669 adults who received treatment between January 2016 and January 2018. Study findings suggest women in our sample were more likely to test positive for benzodiazepines (t = −4.3, p < .001) and amphetamines (t = −4.4, p < .001), whereas men used alcohol at higher rates than women (t = 2.2, p = .026). In addition, women were more likely to report having experienced Post-Traumatic Stress Disorder/trauma (χ2 = 16.5, p < .001) and anxiety (χ2 = 9.4, p = .002). Regression analyses revealed concurrent counseling was unrelated to medication utilization and ongoing opioid use. Patients who had prior counseling utilized buprenorphine more frequently (β = 0.13, p < .001) and used opioids less often (β = −0.14, p < .001); however, both relationships were weak. These data do not provide evidence that counseling during OUD outpatient has a significant impact on treatment outcomes. These findings provide further evidence that barriers to medication treatment such as mandatory counseling can and should be removed.
The Complexity of Educating Acute Care Nurses on Opioid Use Disorder
Authors: Ginther Jane , Chipps Esther , Landers Timothy , Sinnott Loraine , Overcash Janine 
Published: May 2023

Abstract

Background
Opioid use disorder (OUD) is a public health crisis, yet most acute care nurses are not educated to deliver evidence-based OUD care. Hospitalization provides a unique opportunity to initiate and coordinate OUD care in people presenting for other medical–surgical reasons. The aim of this quality improvement project was to determine the impact of an educational program on self-reported competencies of medical–surgical nurses caring for people with OUD at a large academic medical center in the Midwestern United States.

Method
Data were collected from two time points using a quality survey examining self-reported nurse competencies related to (a) assessment, (b) intervention, (c) treatment recommendation, (d) resource use, (e) beliefs, and (f) attitudes toward caring for people with OUD.

Results
Nurses surveyed before education (T1G1, N = 123) and, after education, those who received the intervention (T2G2, N = 17) and those who did not (T2G3, N = 65) were included. Resource use subscores increased over time (T1G1: x = 3.83, T2G3: x = 4.07, p = .006). Results from the two measurement points found no difference in mean total scores (T1G1: x = 3.53, T2G3: x = 3.63, p = .09). Comparison of mean total scores of nurses who directly received the educational program with those who did not during the second time point showed no improvement (T2G2: x = 3.52, T2G3: x = 3.63, p = .30).

Conclusions
Education alone was insufficient in improving self-reported competencies of medical–surgical nurses caring for people with OUD. Findings can be used to inform efforts to increase nurse knowledge and understanding of OUD and to decrease negative attitudes, stigma, and discriminatory behaviors perpetuating care.