bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical use conditions of lead deployment and simulated lead fracture rate in left bundle branch area pacing
Authors: Zou Jiangang , Chen Keping , Liu Xingbin , Xu Yuanning , Jiang Lingyun , Dai Yan , Lin Jinxuan , Hou Xiaofeng , Qiu Yuanhao , Himes Adam , Lucas Ryan , Demmer Wade , Mara Nicki , Zhou Xiaohong , Lu Hongyang 
Published: February 2023

AbstractIntroductionLeft bundle branch area pacing (LBBAP) is achieved by advancing the lead tip deep in the septum. Most LBBAP implants are performed using the Medtronic SelectSecure™ MRI SecureScan™ Model 3830 featuring a unique 4 Fr fixed helix lumenless design. Details of lead use conditions and long‐term reliability have not been reported. This study was designed to quantify the mechanical use conditions for the 3830 lead during and after LBBAP implant, and to evaluate reliability using bench testing and simulation.MethodsFifty bradycardia patients with implantation of the 3830 lead for LBBAP were enrolled. Use conditions of lead deployment at implantation were collected and computed tomography (CT) scans were performed at 3‐month follow‐up. Curvature amplitude along the pacing lead was determined with CT images. Fatigue bending was performed using accelerated testing in a more severe environment than routine clinical use conditions. Conductor fracture rate in a simulated patient population was estimated based on clinical use conditions and fatigue test results.ResultsThe number of attempts to place the 3830 lead for LBBAP was 2.1 ± 1.3 (range: 1–7) with 13 ± 6 lead rotations at the final attempt. Extreme implant conditions were simulated in bench testing with 5 applications of 20 turns followed by up to 400 million bending cycles. Reliability modeling predicted a 10‐year fracture rate of 0.02%.ConclusionsLBBAP implants require more lead rotations than standard pacing implants and result in unique lead bending. Application of simulated LBBAP use conditions to the 3830 lead in an accelerated in‐vitro model does not produce excess conductor fractures.IMAGE‐LBBP Study ID of ClinicalTrial.GOV: NCT04119323.
Catheter ablation of atrioventricular nodal reentrant tachycardia with an irrigated contact‐force sensing radiofrequency ablation catheter
Authors: Panday Priya , Holmes Douglas , Park David S. , Jankelson Lior , Bernstein Scott A. , Knotts Robert , Kushnir Alexander , Aizer Anthony , Chinitz Larry A. , Barbhaiya Chirag R. 
Published: February 2023

AbstractIntroductionRadiofrequency ablation (RFA) slow pathway modification for catheter ablation of atrioventricular nodal reentrant tachycardia (AVNRT) is traditionally performed using a 4‐mm nonirrigated (NI) RF ablation catheter. Slow pathway modification using irrigated, contact‐force sensing (ICFS) RFA catheters has been described in case reports, but the outcomes have not been systematically evaluated.MethodsAcute procedural outcomes of 200 consecutive patients undergoing slow pathway modification for AVNRT were analyzed. A 3.5‐mm ICFS RFA catheter (ThermoCool SmartTouch STSF, Biosense Webster, Inc.) was utilized in 134 patients, and a 4‐mm NI RFA catheter (EZ Steer, Biosense Webster, Inc.) was utilized in 66 patients. Electroanatomic maps were retrospectively analyzed in a blinded fashion to determine the proximity of ablation lesions to the His region.ResultsThe baseline characteristics of patients in both groups were similar. Total RF time was significantly lower in the ICFS group compared to the NI group (5.53 ± 4.6 vs. 6.24 ± 4.9 min, p = 0.03). Median procedure time was similar in both groups (ICFS, 108.0 (87.5–131.5) min vs. NI, 100.0 (85.0–125.0) min; p = 0.2). Ablation was required in closer proximity to the His region in the NI group compared to the ICFS group (14.4 ± 5.9 vs. 16.7 ± 6.4 mm, respectively, p = 0.01). AVNRT was rendered noninducible in all patients, and there was no arrhythmia recurrence during follow‐up in both groups. Catheter ablation was complicated by AV block in one patient in the NI group.ConclusionSlow pathway modification for catheter ablation of AVNRT using an ICFS RFA catheter is feasible, safe, and may facilitate shorter duration ablation while avoiding ablation in close proximity to the His region.
Synergistic effect of PARP inhibitor and BRD4 inhibitor in multiple models of ovarian cancer
Authors: Huang Yuhan , Liu Chen , You Lixin , Li Xi , Chen Gang , Fan Junpeng 
Published: February 2023

AbstractOvarian cancer has the highest facility rate among gynaecological tumours. Current therapies including PARP inhibitors have a defect that ovarian tumour is easy to recurrent and become resistant to therapy. To solve this problem, we found that BRD4 inhibitor AZD5153 and PARP inhibitor olaparib had a widespread synergistic effect in multiple models with different gene backgrounds. AZD5153 sensitizes cells to olaparib and reverses the acquired resistance by down‐regulating PTEN expression levels to destabilize hereditary materials. In this study, we used the following multiple ovarian cancer models PDX, PDO and 3D/2D cell lines to elucidate the co‐effect of AZD5153 and olaparib in vivo and in vitro. The similar results of these models further proved that the mechanism identified was consistent with the biological process occurring in ovarian cancer patients after drug treatment. This consistency between the results of different models suggests the possibility of translating these laboratory research findings into clinical studies towards developing treatments.
Drying of peaches by a combination of convective and microwave methods
Authors: Chatzilia Theodora , Kaderides Kyriakos , Goula Athanasia M. 
Published: February 2023

AbstractThe aim of this work is to study a method to extend the storage life of peaches reducing energy and time consumption and ensuring food quality. The conventional method of hot air dying presents many drawbacks, whereas the microwave drying usually leads to overheating and textural damage. Thus, in this work, a combination of convective and microwave methods, more specifically a two‐stage drying process with an initial convective air drying followed by a microwave drying (microwave finishing), was studied for the drying of peaches. Peaches dried at 70°C for various times, 200, 278, 355, 433, and 510 min, were then dried in a microwave oven at powers of 300, 500, and 800 W. Drying rates were examined, whereas dry peaches were characterized for their color, antioxidant capacity, shrinkage, and rehydration ability under optimized conditions for the situations with and without pretreatment with ascorbic acid. Mass transfer of water was described by nine empirical models and by the diffusional approach. The shrinkage of peach tissue during microwave finishing was evaluated and diffusion coefficients were assessed with shrinkage considered. The microwave finishing reduced the drying time by 89 ± 40%, while a smaller shrinkage was observed, with better rehydration characteristics.Practical applicationsPeaches are important commodities in terms of global agricultural production. As peach is a perishable product with a short shelf‐life, the food industry has been forced to find methods, such as drying, that extend its storage life by months. The conventional method of hot air dying presents many disadvantages. Last decades, microwave drying proposed an alternative way to improve the quality of dehydrated products. However, microwave is rarely used alone as a drying method and is usually combined with other drying techniques. The main goal of this work is to study an innovative method to extend the storage life of peaches reducing energy and time consumption and ensuring food quality.
A new measure of physicians' erroneous assumptions towards adults with intellectual disability: A first study
Authors: Bacherini A. , Havercamp S. M. , Balboni G. 
Published: February 2023

AbstractBackgroundIncomplete knowledge and unfamiliarity with intellectual disability (ID) contribute to erroneous assumptions of physicians towards ID, which negatively impact the health equity of people with ID. This study aimed to identify the erroneous assumptions that, based on the ID stakeholders' perceptions, were the most prevalent in physicians and damaging for the healthcare of adults with ID, verify their unidimensionality and that no personal characteristics of ID stakeholders were associated with their ratings of erroneous assumptions' prevalence and damage.MethodsSeventy‐four possible physician erroneous assumptions were developed concerning health, daily living skills and quality of life of individuals with ID. ID stakeholders rated each one for perceived prevalence in physicians and damage for the healthcare of adults with ID. Frequency analysis, exploratory factor analysis and correlations were run separately for participants' prevalence and damage ratings.ResultsTwenty‐seven erroneous assumptions were identified as those perceived most prevalent and damaging. Their unidimensionality was ascertained and participants' characteristics were not associated with their prevalence and damage ratings.ConclusionsThe identified assumptions are appropriate to represent the items of a new instrument that can be used in medical education to guide the development of curricula to change erroneous assumptions.
Global ideals and restorative extraction: Negotiated Indigeneity on the margins of a Peruvian copper mine
Authors: Gilfoy Kieran 
Published: February 2023

AbstractThis article elucidates the unintended outcomes produced by extractive industries and Indigenous activism in eastern Apurímac, Peru. The article documents the rise and fall of an identity‐based organization in the shadow of the Bambas copper mine using the concept of emergence amid Indigenous spaces to interpret the negotiated products of ideals and realities. I show how notions of historical debt and desires for “a better life” undergird the lived experience of Cotabamban peoples, placing particular demands upon any Indigenous awakening. Over time, creative alterations to activist endeavors shift from a right to culture toward a right to action, infusing artisanal mining ventures with moral credibility. Restorative extraction becomes an Indigenous practice of redress, tackling realities of marginalization and national reciprocity that have been exacerbated by corporate development. Rather than articulating an acceptable identity, restorative extraction temporarily redeems prolonged states of exclusion pertinent for certainpotentiallyIndigenous communities enmeshed in resource booms.
Sex‐specific susceptibility to psychotic‐like states provoked by prenatal THC exposure: Reversal by pregnenolone
Authors: Frau Roberto , Melis Miriam 
Published: February 2023

AbstractSociocultural attitudes towards cannabis legalization contribute to the common misconception that it is a relatively safe drug and its use during pregnancy poses no risk to the fetus. However, longitudinal studies demonstrate that maternal cannabis exposure results in adverse outcomes in the offspring, with a heightened risk for developing psychopathology. One of the most reported psychiatric outcomes is the proneness to psychotic‐like experiences during childhood. How exposure to cannabis during gestation increases psychosis susceptibility in children and adolescents remains elusive. Preclinical research has indicated that in utero exposure to the major psychoactive component of cannabis, delta‐9‐tetrahydrocannabinol (THC), deranges brain developmental trajectories towards vulnerable psychotic‐like endophenotypes later in life. Here, we present how prenatal THC exposure (PCE) deregulates mesolimbic dopamine development predisposing the offspring to schizophrenia‐relevant phenotypes, exclusively when exposed to environmental challenges, such as stress or THC. Detrimental effects of PCE are sex‐specific because female offspring do not display psychotic‐like outcomes upon exposure to these challenges. Moreover, we present how pregnenolone, a neurosteroid that showed beneficial properties on the effects elicited by cannabis intoxication, normalizes mesolimbic dopamine function and rescues psychotic‐like phenotypes. We, therefore, suggest this neurosteroid as a safe “disease‐modifying” aid to prevent the onset of psychoses in vulnerable individuals. Our findings corroborate clinical evidence and highlight the relevance of early diagnostic screening and preventative strategies for young individuals at risk for mental diseases, such as male PCE offspring.
Association between maternal psychological distress and children's neurodevelopment in offspring aged 4 years in Japan: The Tohoku Medical Megabank Project Birth and Three‐Generation Cohort Study
Authors: Takahashi Ippei , Obara Taku , Kikuchi Saya , Kobayashi Mika , Ishikuro Mami , Murakami Keiko , Ueno Fumihiko , Noda Aoi , Onuma Tomomi , Matsuzaki Fumiko , Kobayashi Natsuko , Hamada Hirotaka , Iwama Noriyuki , Saito Masatoshi , Sugawara Junichi , Tomita Hiroaki , Kure Shigeo , Yaegashi Nobuo , Kuriyama Shinichi 
Published: February 2023

AimAn association between maternal psychological distress and children's development has been reported, but reports from Japan are limited. This study aimed to examine the association of maternal psychological distress with children's neurodevelopment in Japan.MethodsThe study assessed data of 7646 mother–infant pairs in the Japanese population. We used Kessler Psychological Distress Scale, a screening tool for psychological distress, to assess maternal psychological distress in early pregnancy and 2 years postpartum and divided it into four categories: none in both the pre‐natal and post‐natal periods, only the pre‐natal period, only the post‐natal period and both the pre‐natal and post‐natal periods. Children's neurodevelopment was assessed using the Ages & Stages Questionnaires Third Edition (ASQ‐3) at 4 years of age. ASQ‐3 comprises five domains (communication, gross motor, fine motor, problem solving and personal–social), and the score of less than −2 standard deviation relative to the mean in reference was defined as having developmental delay. We conducted multivariate logistic regression analysis to examine the association between maternal psychological distress and children's neurodevelopment.ResultsThe prevalence of developmental delay of communication, gross motor, fine motor, problem solving and personal–social were 4.0%, 4.3%, 4.9%, 3.8% and 4.6%, respectively. Maternal psychological distress in only the postpartum period and both pre‐natal and postpartum periods were associated with risks of developmental delay in all domains. Maternal psychological distress in only the pre‐natal period was associated with developmental delay in communication.ConclusionsMaternal psychological distress is associated with risks of children's developmental delay.
Coexistence of grasses and shrubs in Patagonian steppes. Norm or exception?
Authors: Biancari Lucio , Oñatibia Gastón R. , Gaitán Juan J. , Aguiar Martín R. 
Published: February 2023

AbstractQuestions(1) Is the co‐dominance of grasses and shrubs a general feature of Patagonian steppes? (2) At a local scale, does it change with domestic grazing intensity or decadal changes in precipitation?LocationsQuestion 1. Patagonia region in southern South America. Question 2. Shrub–grass steppes (45°24′S, 70°17′W) subjected to different grazing intensity for decades.MethodsWe sampled vegetation to estimate perennial grass and shrub cover at two scales: regional and local. At the regional (phytogeographical) scale, we sampled grazed steppes under different climates and soils (n = 324 sites over ca. 487,000 km2). At the local scale, we sampled paddocks subjected to known grazing management, from commercial intensive densities of sheep to total (experimental) exclusion. Also, in the same site, we sampled permanent plots (25 years old) in a grazing exclosure to study the effects of precipitation variation.ResultsMixed communities are the predominant form of community organization in the Patagonia region. At the regional scale, shrub cover was negatively related to grass cover and communities where shrubs had higher cover than grasses represent 54% of the data set. At the local scale for an arid steppe, perennial grasses had higher cover than shrubs, regardless of grazing pressure. The shrub‐to‐grass ratio was increased only by intensive grazing. Permanent plots in a grazing‐excluded paddock indicate that decadal xeric or humid climate periods decrease or increase total cover, respectively. Surprisingly, the relative shrub‐to‐grass ratio did not change significantly.ConclusionsAlthough perennial grasses and shrubs cover are negatively correlated, they coexist in the Patagonian steppes under a wide range of climate, soil and grazing conditions. At a local scale, selective herbivory promotes species replacement but did not substantially change the life‐form covers. Water shortages, as well as periods of water surplus, similarly affect both life forms without compromising their coexistence.
Icos gene disruption in non‐obese diabetic mice elicits myositis associated with anti‐troponin T3 autoantibodies
Authors: Bourdenet Gwladys , Pileyre Baptiste , Drouot Laurent , Martinet Jérémie , Bécourt Chantal , Carrette Marion , Riou Gaétan , Bergua Cécile , Jaworski Thara , Chan Philippe , Jean Laetitia , Fréret Manuel , Cosette Pascal , Boitard Christian , Abad Catalina , Boyer Olivier 
Published: February 2023

AbstractAimsIdiopathic inflammatory myopathies (IIM) are autoimmune inflammatory disorders leading to skeletal muscle weakness and disability. The pathophysiology of IIM is poorly understood due to the scarcity of animal disease models. Genetic deletion of Icos or Icosl (inducible T cell co‐stimulator/ligand) in non‐obese diabetic (NOD) mice leads to muscle disease. Our aim was to characterise Icos−/− NOD myopathy and to search for novel autoantibodies (aAbs) in this model.MethodsDiabetes, weight, myopathy incidence/clinical score and grip strength were assessed over time. Locomotor activity was analysed with the Catwalk XT gait analysis system. Muscle histology was evaluated in haematoxylin/eosin and Sirius red‐stained sections, and immune infiltrates were characterised by immunofluorescence and flow cytometry. 2D gel electrophoresis of muscle protein extracts and mass spectrometry were used to identify novel aAbs. NOD mice were immunised with troponin T3 (TNNT3) in incomplete Freund's adjuvant (IFA) and R848. An addressable laser bead immunoassay (ALBIA) was developed to measure aAb IgG serum levels.ResultsIcos−/− NOD mice did not exhibit diabetes but developed spontaneous progressive myositis with decreased muscle strength and altered locomotor activity. Muscle from these mice exhibited myofibre necrosis, myophagocytosis, central nuclei, fibrosis and perimysial and endomysial cell infiltrates with macrophages and T cells. We identified anti‐TNNT3 aAbs in diseased mice. Immunisation of NOD mice with murine TNNT3 protein led to myositis development, supporting its pathophysiological role.ConclusionsThese data show that Icos−/− NOD mice represent a spontaneous model of myositis and the discovery of anti‐TNNT3 aAb suggests a new autoantigen in this model.
Liberalism, nationalism and federalism: Reflections on the life and work of Meïr Goldschmidt
Authors: Johnsen Alex Grøn , Hall John A 
Published: February 2023

AbstractThe Danish‐Jewish publisher Meïr Aron Goldschmidt was an active political voice in Denmark in the 1840s and 1850s during the crisis of the Danish Oldenburg monarchy, when the ‘Danish Empire’ was troubled by territorial defragmentation, succession crisis, foreign military threats, Danish–German ethnic tensions and calls for democratic reforms by National Liberals.This article reflects on Goldschmidt's life and works as he attempted to syncretise the inherent dualities of nationalism and liberalism. His vision became a Swiss‐inspired federalism that should create a shared national identity based on liberal democracy to reunite the ethnic groups of the ‘Danish Empire’.Ultimately, history took another course, and the nationalist path was taken with disastrous results for Denmark. However, Goldschmidt has left a legacy in his writings as a microcosm of the ideas of his time by trying to syncretise nationalism and liberalism, cosmopolitanism and nationalism and a Danish and Jewish national identity.
Foliar nutrient resorption stoichiometry and microbial phosphatase catalytic efficiency together alleviate the relative phosphorus limitation in forest ecosystems
Authors: Peng Ziyang , Wu Yuntao , Guo Lulu , Yang Lu , Wang Bin , Wang Xin , Liu Weixing , Su Yanjun , Wu Jin , Liu Lingli 
Published: February 2023

Summary
Understanding how plants adapt to spatially heterogeneous phosphorus (P) supply is important to elucidate the effect of environmental changes on ecosystem productivity. Plant P supply is concurrently controlled by plant internal conservation and external acquisition. However, it is unclear how climate, soil, and microbes influence the contributions and interactions of the internal and external pathways for plant P supply.
Here, we measured P and nitrogen (N) resorption efficiency, litter and soil acid phosphatase (AP) catalytic parameters (Vmax(s) and Km), and soil physicochemical properties at four sites spanning from cold temperate to tropical forests.
We found that the relative P limitation to plants was generally higher in tropical forests than temperate forests, but varied greatly among species and within sites. In P‐impoverished habitats, plants resorbed more P than N during litterfall to maintain their N : P stoichiometric balance. In addition, once ecosystems shifted from N‐limited to P‐limited, litter‐ and soil‐specific AP catalytic efficiency (Vmax(s)/Km) increased rapidly, thereby enhancing organic P mineralization.
Our findings suggested that ecosystems develop a coupled aboveground–belowground strategy to maintain P supply and N : P stoichiometric balance under P‐limitation. We also highlighted that N cycle moderates P cycles and together shape plant P acquisition in forest ecosystems.
miR394 enhances WUSCHEL‐induced somatic embryogenesis in Arabidopsis thaliana
Authors: Lu Lu , Holt Anna , Chen Xinying , Liu Yang , Knauer Steffen , Tucker Elise J. , Sarkar Ananda Kumar , Hao Zhaodong , Roodbarkelari Farshad , Shi Jisen , Chen Jinhui , Laux Thomas 
Published: February 2023

Summary
Many plant species can give rise to embryos from somatic cells after a simple hormone treatment, illustrating the remarkable developmental plasticity of differentiated plant cells. However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro‐RNA394 (miR394) promotes shoot meristem maintenance in Arabidopsis thaliana, but the underlying mechanisms have remained elusive.
We analyzed whether miR394 affects indirect somatic embryogenesis and determined the transcriptome of embryogenic callus upon miR394‐enhanced somatic embryogenesis.
We show that ectopic miR394 expression enhances somatic embryogenesis in the recalcitrant Ler accession when co‐expressed with the transcription factor WUSCHEL (WUS) and that miR394 acts in this process through silencing the target LEAF CURLING RESPONSIVENESS (LCR). Furthermore, we show that higher endogenous miR394 levels are required for the elevated embryogenic potential of the Columbia accession compared with Ler, providing a mechanistic explanation for this natural variation.
Our transcriptional analysis provides a framework for miR394 function in regulating pluripotency by expanding WUS‐mediated direct transcriptional repression.