bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Absorption LIDAR Signal Denoising using Empirical Mode Decomposition Technique
Authors: Jindal M K , Mainuddin M , Veerabuthiran S , Jindal N 
Published: May 2023

Abstract
Differential Absorption Lidar (DIAL) technique is a potential method for the remote detection of hazardous chemicals in the atmosphere. These hazardous chemicals can be due to industrial pollution or may be intentionally released by the terrorist groups or military forces of the enemy country to endanger both the military's personnel and the civilian population's lives. DIAL technique may be used for probing such chemicals from far-off distances of several km ranges and generating an early warning for the response teams. The output of the DIAL system normally consists of three parameters viz. name/class of hazardous chemical detected; its location in terms of distance and the concentration. The maximum standoff distance capability for any Lidar system depends on the signal to noise ratio which is governed by the parameters like atmospheric conditions, Lidar subsystem specifications, noises, etc. SNR is often limited by several noises embedded in the signal from various sources. Due to the presence of noises in the signal, the errors are introduced in the concentration estimation of chemicals from Lidar signal. The methods for improvement of SNR of lidar signal has been often limited by application of conventional denoising techniques like multi-pulse temporal averaging and spatial averaging and further requires nonlinear techniques for noise reduction due to nonlinear behavior of lidar signals. In the present work, Empirical Mode Decomposition (EMD) technique has been implemented on the Lidar signal from Differential Absorption Lidar system. The signal has been denoised and improved SNR is compared with that achieved from temporal averaging and spatial averaging. It was observed that the EMD technique is a better technique as compared to other conventional techniques like multi-pulse temporal averaging and spatial averaging for denoising the signal and increasing Lidar SNR. It is seen that SNR can be improved 4–5 times the original SNR using EMD technique.
Potential of LAMP assay as a tool for ecological monitoring and on-site detection of Bipolaris oryzae in rice
Authors: Logeshwari R. , Gopalakrishnan C. , Kamalakannan A. , Ramalingam J. , Saraswathi R. 
Published: May 2023

Abstract
One of the most significant rice seed-borne pathogens, Bipolaris oryzae causes serious yield losses worldwide every year. Traditional seed health testing involves incubation for up to a week, but molecular diagnosis using PCR-based assays requires sophisticated equipment and is not amenable to on-site testing. Loop-mediated isothermal amplification (LAMP) is ideal for field-level diagnosis since it offers quick, high-specific amplification of target template sequences at a single temperature. The exon 2 of the Glycosyl Transferase Family 90 protein of B. oryzae was targeted for designing the LAMP primers. The LAMP assay proposed was incredibly sensitive, detecting the B. oryzaetemplate's presence at concentrations as low as 10 fg at 60 °C in 30 min. The specificity of the test was assessed using genomic DNA retrieved from 22 different phytopathogens. The assay was employed to biologically monitor the existence of this pathogen by testing it on ten weed species in rice fields infected with B. oryzae. The pathogen was detected in 8 weed species except Brachypodium distachyon and Phalaris minor. The assay was used to detect the presence of B. oryzae in seeds from 13 different rice varieties. Seed samples from two cultivars tested negative while the remaining cultivars were confirmed to harbor the pathogen. Using this novel assay, B. oryzae can be detected rapidly in rice seeds and farm soil. The proposed LAMP assay has the potential to be employed in seed health laboratories, plant quarantine stations and on-site detection of B. oryzae in seeds and plants.
WITHDRAWN: SWAST KHOJ: A real-time working prototype for vital healthcare monitoring System
Authors: Saha Ramesh , Bharadwaj Sanjeev Kumar , Saif Sohail , Ghosh Rajdeep , Biswas Suparna , Karmakar Sushanta 
Published: May 2023

Abstract
Internet of Things (IoT)-based health monitoring system is centred on continuous, real-time monitoring of the health of individuals. The emergence of IoT-enabled healthcare devices is rapidly changing the health infrastructure. IoT-enabled technologies can facilitate an effortless interaction among different devices and platforms. Smart health monitoring applications are a notable and important application in IoT and therefore, different types of IoT frameworks have been proposed by researchers. In the present work, an IoT–based real-time healthcare prototype has been implemented using unobtrusive heterogeneous sensor nodes to create a Wireless Body Area Network (WBAN) architecture. The prototype has been designed to collect vital parameters of the human body using heterogeneous sensors like: electrocardiogram (ECG), temperature, Saturation of peripheral oxygen (SPO2), and pulse rate. The collected data from the prototype shows the condition of the patient in a graphical user interface (GUI) in compliance with the Modified Early Warning Score (MEWS) medical guidelines. The prototype along with the user interface is named SWAST KHOJ. The data is periodically uploaded with the timestamp information on the local server. Further, the Quality of Service (QoS) parameter of the prototype is evaluated for different short-range communication like LAN (or Wire), ZigBee, and Bluetooth in an indoor environment. The performance of the proposed prototype is evaluated on different communication technologies and it was observed that the proposed prototype requires an end-to-end delay of 0.514 ms, 0.62 ms, 0.417 ms and 1.92 ms for wire, Wi-Fi, ZigBee and Bluetooth respectively. Also, it is observed that the end-to-end delay of the proposed method is less than that of the previous works. Moreover, the throughput of the prototype using different communication technology has been evaluated for the prototype.
Enolase2(ENO2) responsed Perfluorooctane Sulfonate toxic in Rattus norvegicus Brain
Authors: Li Honghui , Yang Huifang , Ma Zhanbing 
Published: May 2023

Abstract
Perfluorooctane sulfonate (PFOS) is a global environmental contaminant known to cause neurotoxicity. However, the specific targets of its neurotoxic effects remain poorly understood. In this study, we aimed to investigate the differential protein expression associated with brain tissue toxicity induced by potassium perfluorooctane sulfonate (PFOS-K) and predict its potential biological functions. Rattus norvegicus were treated with 10 mg/kg PFOS-K for 30 days to establish a toxicity model, and brain tissue morphology was examined using HE and nissl staining. Protein expression profiles were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Bioinformatics tools were used to analyze protein functions, and immunofluorescence was employed to detect the expression of the ENO2 gene in whole brain tissue.PFOS exposure resulted in changes in neuronal cell morphology in different regions of the rat hippocampus, including cell vacuolation, reduced neuronal axon length and density, and decreased nisomes in the DG region. PFOS-K exposure led to changes in protein expression profiles in brain tissue, with 53 differentially expressed proteins identified. Gene ontology (GO) and pathway analysis revealed that the main function of these proteins is to participate in the mitochondrial oxidative respiratory electron transport chain. The main signaling pathways involved include glycolysis, glutamine conversion, Wnt signaling pathway, energy metabolism, oxidative stress, and inflammatory-mediated cytokine signaling. PFOS-K exposure may primarily promote mitochondrial oxidative respiratory electron transport chain, elevate cytoplasmic Glu levels, and disrupt Ga2+ homeostasis. It induces mitochondrial pathway-dependent apoptosis, while also significantly down-regulating the expression of the neurotrophic and protective factor ENO2 in the pyramidal layer of the hippocampus, which may ultimately result in neuronal pathological damage and brain tissue toxicity through energy metabolism.
Wave or Particle? Quantum Behavior of Solar System
Authors: Liang Zhong-Cheng , Xie Ling-Hai 
Published: May 2023

Abstract
Starting from the Kepler's laws of planetary motion, this paper applies the real-particle theory to study the motion property and quantum behavior of the solar system. The earth/solar system is a typical two-body cluster (real particle), which can be modeled as a rotating vibrator. The classical state function of the vibrator is an elliptical equation, and the quantum state function is a topological mapping of the elliptical equation in the complex space. The quantum state function contains all the information of the cluster motion, and the probabilities of various motion modes are controlled by particle interactions and satisfies the Schrodinger equation. The study shows that the quantum state function characterizes the long-time statistical properties of cluster motion, and the Schrodinger equation is a mapping algorithm from potential function to state function. The parameters of the elliptical orbit are implicit variables of the quantum state, and the statistics of cluster motion is the reality basis of quantum mechanics.
1,3,5-Triazine Based Hydroxamic Acid Analogues as HDAC Inhibitors: Integrated Modelling by 3D QSAR, Hypogen Pharmacophore Based Virtual Screening and Molecular Dynamic Simulation Studies
Authors: Patel Preeti , Navneesh N , Kurmi BALAK , Rangra Naresh Kumar , Gupta Ghanshyam Das , Rawat Ravi , Pagano Mario Angelo , Brunati Anna Maria , Rajak Harish 
Published: May 2023

Abstract
Histone deacetylase (HDAC) is one of the therapeutically relevant target for the treatment of various types of cancer. To establish a correlation between biological potency and their structural features using a combined approach of Hypogen pharmacophore and five different 3D QSAR models. Hypogen model (Hypo1) for HDAC inhibitors was developed on the basis of existing one, having good correlation coefficient (R= 0.76), lowest root mean square deviation (RMSD) of 0.91, maximum fit value of 9.77 and highest cost difference value of 66.86. Quality validation of Hypo1 through cost analyses, test set prediction and Fischer’s randomization test suggested that the model can reliably detect HDAC inhibitors. The five different 3D QSAR models i.e., Recursive Partitioning Classification Model (pkRP model), Multiple Linear Regression (MLR) analysis, Partial Least Square (PLS), Genetic Function Approximation (GFA) models and Field based (or Grid Based) QSAR models were created and validated. Furthermore, top scored compounds were filtered through the virtual screening and after that, molecular dynamic (MD) simulation and MM-GBSA studies were used to validate the stability, binding energy and electrostatics of best HDAC receptor-ligand complex. The outcome of findings indicated that the best Field based model has R2=0.970and Q2=0.842, the best pkRP model has R2= 0.883 and Q2= 0.813, the best PLS model has R2= 0.856 and Q2= 0.746,the best MLR model has R2= 0.766 and Q2= 0.752, and the best GFA model has R2= 0.706 and Q2= 0.663. Cross-validated coefficient, (rcv2) of 0.968, 0.827, 0.743, 0.810 and 0.552 was found for Field/Grid based, pkRP, MLR, PLS and GFA model, respectively indicating the satisfied correlativity and prediction. The outcome of these studies will be a focal paradigm for designing of novel lead molecules as HDAC inhibitors with improved anticancer activity.
Development of an Ejector for Passive Hydrogen Recirculation in PEM Fuel Cell Systems by applying 2D CFD Simulation
Authors: Singer Gerald , Köll Rebekka , Pertl Patrick , Trattner Alexander 
Published: May 2023

Abstract
The anode subsystem is a major energy consumer of Polymer-Electrolyte-Membrane (PEM) fuel cell systems. A passive hydrogen recirculation system, like an ejector, is an excellent solution to maximize hydrogen utilization while maintaining low parasitic losses. However, high development efforts are necessary to maximize the performance of the ejector for the entire operating range. This research paper provides part of a toolchain for ejector development, consisting in particular of a multi-parameter simulation based on rotational symmetric 2D CFD. The 2D CFD greatly helps optimize the design of the ejector, reducing development effort, and increasing accuracy. In addition, the main correlations between thermodynamic states and geometry on the entrainment ratio are evaluated. Subsequently, an ejector is designed for a PEM fuel cell application using 2D CFD and the results show in which operating range a single ejector can be applied. This toolchain enables rapid design and optimization of ejector geometry, saving development time and cost while increasing accuracy and extending the operating range.
Optimization of Methylene Blue Dye Removal Adsorbed on Olive Pomace Derived Activated Charcoal: Isotherm, Kinetic and Thermodynamic Studies
Authors: Alouiz Imad , Benhadj Mohssine , Elmontassir Dahmane , Mouadili Abdeslam , Sennoune Mohamed , Amarouch Mohamed-Yassine , Mazouzi Driss 
Published: May 2023

Abstract
Methylene blue (MB) is one of dyes used in the industry. However, these dyes contribute generally to water pollution. The continued accumulation of these organic compounds has had a significant and negative harmful on the environment and human health. Therefore, many biological, physical and chemical processes have been developed aiming to eliminate these compounds from the environment. Of these processes, adsorption is the most well-known one. In this context, this study aims to assess the absorbent properties of an olive pomace derived activated charcoal (ACp) against the methylene blue dye. For this purpose, to confirm MB adsorption on ACp structures, SEM, EDX and FTIR analysis were performed. The influence of dye concentration, contact time, pH, adsorbent dose and temperature were studied to evaluate the removal efficiency of MB by adsorption. In which, kinetic and isothermal models were used to better understand the adsorption mechanism. A good correlation has been indicated with the pseudo-second-order kinetic model and Langmuir isotherm. Also, thermodynamic result related to the adsorption process indicating physisorption, endothermic, spontaneous, and favorable.
The combination of kidney function variables with cell cycle arrest biomarkers identifies distinct subphenotypes of sepsis-associated acute kidney injury: a post-hoc analysis (the PHENAKI study)
Authors: Titeca-Beauport Dimitri , Diouf Momar , Daubin Delphine , Vong Ly Van , Belliard Guillaume , Bruel Cédric , Zerbib Yoann , Vinsonneau Christophe , Klouche Kada , Maizel Julien 
Published: May 2023

Abstract
Background: The severity and course of sepsis-associated acute kidney injury (SA-AKI) are correlated with the mortality rate. Early detection of SA-AKI subphenotypes might facilitate the rapid provision of individualized care.
Patients and methods: In this post-hocanalysis of a multicentre prospective study, we combined conventional kidney function variables (the pre-admission serum creatinine (SCr) and estimated glomerular filtration rate, SCr on inclusion (0 h) and at 24 h, blood urea nitrogen at 0 h, and the weight-corrected urine output (UO) at 0, 6, 12 and 24 h) with serial measurements of urine [tissue inhibitor of metalloproteinase-2 (TIMP-2)]*[ insulin-like growth factor-binding protein (IGFBP7)] at 0, 6, 12 and 24 h) and then using an unsupervised hierarchical clustering of principal components approach to identify different phenotypes of SA-AKI. We then compared the subphenotypes with regard to the renal replacement therapy initiation rate and survival rate in the first seven days.
Results: We included 184 patients presenting SA-AKI within six hours of the diagnosis of septic shock. Three distinct subphenotypes were identified: subphenotype A (99 patients) was characterized by a normal UO, a low SCr and a low [TIMP-2]*[IGFBP7] level; subphenotype B (74 patients) was characterized by existing chronic kidney disease, a higher SCr, a low UO, and an intermediate [TIMP-2]*[IGFBP7] level; and subphenotype C was characterized by very low UO, a very high [TIMP-2]*[IGFBP7] level, and an intermediate SCr level. Renal replacement therapy was initiated within the first seven days in four (4%), 27 (36%) and three (27%) patients from the subphenotype A, B and C groups, respectively (p<0.001). After adjustment for confounding factors, the seven-day survival rate [95% confidence interval (CI)] was respectively 88% [80–96], 80% [72–88] and 75% [56–94] in the subphenotypes A, B and C. With subphenotype A as reference, the adjusted hazard-ratio [95%CI] for seven-day mortality was 1.53 [0.76–3.08] (p=0.24) in subphenotype B and 5.90 [2.04–17.07] (p=0.001) in subphenotype C.
Conclusions: By combining conventional kidney function variables with urine measurements of a cell-cycle arrest biomarker, we identified three distinct SA-AKI subphenotypes with different short-term courses and survival rates. This approach might help to better stratify patients in the early phases of septic shock but its value must be confirmed in a larger, independent cohort.
Trial registration: the AKI-CHECK study was registered at ClinicalTrials.gov (NCT02812784) before the inclusion of the first patient.
Running head: Subphenotypes of sepsis-associated acute kidney injury
Recyclable Magnetic Nickel Ferrite-Carboxymethyl Cellulose-Sodium Alginate Bio-Composite for Efficient Removal of Nickel Ion from water
Authors: Singh Rajinder , Jasrotia Rimzim , Singh Jandeep , Mittal Sunil , Singh Harminder 
Published: May 2023

Abstract
In wastewater treatment, magnetic bio-composites are frequently investigated as an adsorbent due to their great capacity for adsorption and affordability. In this current work, an attempt has been made to develop spinel nickel ferrite carboxymethyl cellulose composite (NiFCMC) and modified its surface by alginate polymer to form NiFCMC-Alg. composite. Several techniques were utilized to characterize these adsorbents including (FTIR), XRD (X-Ray Diffraction), FE-SEM, EDS (energy-dispersive spectra), TGA (thermogravimetric analysis), VSM and pHzpc. These adsorbents were explored to check their potentiality to remove Ni (II) ions in aqueous medium on various parameters such as contact time, initial metal ion concentration, pH, adsorbent dose and temperature. The optimum equilibrium time noticed was 180 minutes at pH 8 with adsorbent dose of 0.1 g. Results of kinetic studies showed best fit result for Lagergren pseudo second order model. Likewise, the Langmuir isotherm model also showed good agreement with maximum adsorption capacities 47.84 mg/g, 60.24 mg/g for NiFCMC and NiFCMC-Alg. respectively. Calculated thermodynamic parameters confirmed spontaneous nature of adsorption process. The regeneration efficiency of both adsorbents was studied for five cycles. This study has shown that NiFCMC and NiFCMC-Alg. can be a good substitute for removing Ni (II) ions in aqueous medium.
Diversity and Functionality of Bacterial Symbionts in Different Tissues of Spider Heteropoda venatoria Revealed through Integration of High-throughput Sequencing and Culturomic Approaches
Authors: Zhao Likun , Zhang Shanfeng , Xiao Ruoyi , Zhang Chao , Lyu Zhitang , Zhang Feng 
Published: May 2023

Abstract
Spiders host a diverse range of bacteria in their guts and other tissues, which have been found to play a significant role in their fitness. This study aimed to investigate the community diversity and functional charcateristics of four tissue-associated symbiotic bacteria in Heteropoda venatoria using high-throughput sequencing (HTS) of 16S ribosomal RNA (rRNA) gene and culturomic technologies. The results of HTS showed that the symbiotic bacteria in different tissues belonged to 34 phyla, 72 classes, 170 orders, 277 families, and 458 genera. Bacillus was found to be the most abundant bacteria in the venom gland, silk gland, and ovary, while Stenotrophomonas, Acinetobacter and Sphingomonas were dominant in the gut microbiota. Based on the amplicon sequencing results, 21 different cultivation conditions were disigned using culturomics to isolate bacteria from the ovary, gut, venom gland, and silk gland. A total of 119 bacterial strains belonging to 4 phyla and 25 genera, with Bacillus and Serratia as the main genera, were isolated. Functional predictions revealed that metabolism was the most abundant, and in vitro validation experiments showed that five strains had high efficiency in pesticide degrading. Furthermore, 28 of the 119 isolates exhibited antibacterial activity against at least one of the tested bacterial strains, including pathogenic bacteria Staphylococcus aureus, Acinetobacter baumanii and Enterococcus faecalis. The study also identified three strains, GL312, PL211and PL316, which had significant cytotoxicity against MGC-803, with the fermentation crude extract of strain PL316 found to be an effective inducer of MGC-803 cell apoptosis. Overall, this study provides a comprehensive understanding of the community structure of symbiotic bacteria in H. venatoria and offers valuable insights into the discovery of novel antitumor natural products for gastric cancer and xenobiotic degrading bacteria of spiders.
Simultaneous Transcatheter Aortic Valve Replacement and Endovascular Aortic Aneurysm Repair – A Case Report
Authors: Escobar Johao , Alvarenga Carlos , Rivera Mario Rodriguez , Krittanawong Chayakrit 
Published: May 2023

Abstract
Introduction
The case highlights the management of severe aortic stenosis in an elderly patient with multiple comorbidities, where TAVR and EVAR were performed simultaneously to address the patient's complex cardiovascular issues.
Case presentation
An 81-year-old female with a history of various cardiovascular conditions presented with dyspnea, presyncope, and abdominal pain. Imaging revealed severe calcification of the aortic valve and an infrarenal aortic aneurysm. A simultaneous transcatheter aortic valve replacement and endovascular aortic repair were performed, with no complications at the access sites but development of mild paravalvular leak and left bundle branch block were noted. The patient was discharged one week after the procedure.
Conclusion
A patient with severe aortic stenosis and abdominal aortic aneurysm was treated with a successfully combined TAVR and EVAR approach. Simultaneous performance of TAVR and EVAR may help mitigate risks in certain patients, despite limited evidence on outcomes.