bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the adsorption of Arsenic on Single-Walled Carbon Nanotube and Fe-doped Single-Walled Carbon Nanotube: A Quantum Mechanical Study
Authors: Munio Art Anthony Zoilo , Pido Alvanh Alem , Ambolode Leo Cristoba II 
Published: May 2023

Abstract
Due to mounting environmental and public health concerns about the toxicity of Arsenic (As) contamination, there is a strong drive to develop cost-effective sensors and adsorbent material for As. Using density functional theory, we examined the adsorption mechanism, electronic structure, and optical absorption spectra of SWCNT with atomic As and Arsenous acid (H3AsO3). Results indicate that atomic As can strongly interact with SWCNT with significant structural deformation of the SWCNT upon adsorption. This bonding creates modification on the intrinsic electronic structure and the optical absorption spectra of the prototype SWCNT. Hence, SWCNT is an efficient adsorbent and a candidate material for sensing atomic As. On the other hand, H3AsO3 interacts weakly with the SWCNT, with no significant modification observed in the SWCNT's atomic configuration, electronic structure, and optical absorption spectra. The interaction and sensitivity with H3AsO3 significantly improved after doping the SWCNT with Fe. The changes in the band structure patterns and optical absorption spectra of Fe-doped SWCNT is also observed upon exposure to H3AsO3. The results presented here provide fundamental insights into the interaction of SWCNT and As, which serve as a reference for fabricating SWCNT-based adsorbent and sensing platforms of heavy metals. The results further explore how metal-doped SWCNT tunes the bonding and sensitivity with heavy metals.
smart±step exergame and seated computer brain training for preventing falls in community-dwelling older people: a 12-month randomised controlled trial.
Authors: Hicks Cameron , Smith Natassia , Ratanapongleka Mayna , Menant Jasmine , Turner Jessica , Lo Joanne , Garcia Jamie , Valenzuela Michael , Chaplin Carly , Delbaere Kim , Herber Robert , Sherrington Catherine , Toson Barbara , Lord Stephen , Sturnieks Daina 
Published: May 2023

Abstract
Objectives Exergame training is a promising method for delivering evidence-based fall prevention exercise as it can facilitate exercise adherence and can be tailored to address cognitive and motor risk factors for falls. This study examined the effectiveness of two home-based computer game interventions (seated brain training and step training; smart±step) in preventing falls in community-dwelling older people, compared to a minimal-intervention control group.
Design Assessor-blinded, randomised controlled trial.
Setting Research institute and homes of older people living in the community of Sydney, Australia.
Participants 769 people aged 65+ years living independently in the community and free of cognitive impairment, progressive neurological disease, or any other unstable or acute medical condition that precludes exercise.
Interventions Participants were randomised to one of three arms: brain training using a computerised touch pad while seated; exergame step training on a computerised mat; or control. The intervention groups were provided with a home-based training system and were asked to train for at least two hours per week over the 12-month trial.
Main outcome measures The primary outcome was the rate of falls reported monthly over 12 months.
Results Between October 2016 and May 2019, 769 participants were randomised (seated brain training =262, exergame step training=252, control=255) and all were included in the intention-to-treat analyses. The rate of falls over 12 months was significantly reduced in the exergame step training group compared to control (IRR=0.74, 95%CI=0.56 to 0.98) and was not statistically different between the seated brain training and control groups (IRR=0.86, 95%CI=0.65 to 1.12). No serious intervention-related adverse events were reported.
Conclusions A home-based exergame step training program provides a safe and effective means for preventing falls in older people living in the community. As this intervention can be conducted at home and requires only minimal equipment, it has the potential for scalability as a public health intervention to address the increasing problem of falls and fall-related injuries.
Trial registration Australian and New Zealand Clinical Trial Registry ACTRN12616001325493, registered 22 September 2016.
FHL1 as a prognostic biomarker and therapeutic target in acute myeloid leukemia
Authors: Luo Bo , Li Wei , Li Jialing , Chen Guanfei , Zeng Jingyuan , Hu Nan , Li Lulu , He Zhongming , He Shuang , Guo Qulian , Zheng Xiaoli 
Published: May 2023

Abstract
Acute myeloid leukemia (AML) has a poor prognosis and high heterogeneity. Most leukemias are caused by environmental factors interacting with the cell’s genetic material, but treatment is still dominated by cell cycle drugs. Therefore, there is an urgent need to find reliable biomarkers. Based on the Gene Expression Omnibus database, Kaplan–Meier survival analysis and univariate Cox regression analysis were used to select the genes that had the most significant influence on the prognosis of patients with AML. Quantitative real-time PCR and Western blot were used to detect the effect of small interfering RNA transfection and lentiviral interference on knockout and overexpression of the gene, respectively, and to verify the difference in the FHL1 gene in the HL60 cell line and neutrophils. Cell Counting Kit-8 and flow cytometry were used to detect the effect of high or low expression of FHL1 on cell viability and apoptosis under the influence of cytarabine and daunorubicin. FHL1 was found to be the most prognostic independent biomarker by GSE12417 screening and GSE37642 validation. FHL1 is highly expressed in AML, and knockdown of FHL1 can increase the sensitivity of AML cells to cytarabine and daunorubicin. FHL1 may play a role as a potential molecular marker and therapeutic target for predicting poor prognosis of AML and for direct treatment (adjuvant radiotherapy or chemotherapy).
Evidence of Bisphosphonate-Conjugated Sitafloxacin Eradication of Established Methicillin-Resistant S. aureus Infection with Osseointegration in Murine Models of Implant-Associated Osteomyelitis
Authors: Xie Chao , Ren Youliang , Weeks Jason , Xue Thomas , Rainbolt Joshua , Bentley Karen de Mesy , Shu Ye , Liu Yuting , Masters Elysia , Cherian Philip , McKenna Charles , Neighbors Jeffrey , Ebetino Frank , Schwarz Edward , Sun Shuting 
Published: May 2023

Abstract
Eradication of MRSA osteomyelitis requires elimination of distinct biofilms. To overcome this, we developed bisphosphonate-conjugated sitafloxacin (BCS, BV600072) and hydroxybisphosphonate-conjugate sitafloxacin (HBCS, BV63072), which achieve “target-and-release” drug delivery proximal to the bone infection and have prophylactic efficacy against MRSA static biofilm in vitro and in vivo. Here we evaluated their therapeutic efficacy in a murine 1-stage exchange femoral plate model with bioluminescent MRSA (USA300LAC::lux). Osteomyelitis was confirmed by CFU on the explants and longitudinal bioluminescent imaging (BLI) after debridement and implant exchange surgery on day 7, and mice were randomized into seven groups: 1) Baseline (harvested at day 7, no treatment); 2) HPBP (bisphosphonate control for BCS) + vancomycin; 3) HPHBP (bisphosphonate control for HBCS) + vancomycin; 4) vancomycin; 5) sitafloxacin; 6) BCS + vancomycin; and 7) HBCS + vancomycin. BLI confirmed infection persisted in all groups except for mice treated with BCS or HBCS + vancomycin. Radiology revealed catastrophic femur fractures in all groups except mice treated with BCS or HBCS + vancomycin, which also displayed decreases in peri-implant bone loss, osteoclast numbers, and biofilm. To confirm this, we assessed the efficacy of vancomycin, sitafloxacin, and HBCS monotherapy in a transtibial implant model. The results showed complete lack of vancomycin efficacy, while all mice treated with HBCS had evidence of infection control, and some had evidence of osseous integrated septic implants, suggestive of biofilm eradication. Taken together these studies demonstrate that HBCS adjuvant with standard of care debridement and vancomycin therapy has the potential to eradicate MRSA osteomyelitis.
One-stage Circumcision and Hyaluronic acid injections for Men Reporting Small Penis with Redundant Prepuce or Phimosis
Authors: He Hailong , Li Qing , Zhang Xiaowei 
Published: May 2023

Abstract
Background The efficacy and safety of filler injection were approved and have become the main choice for penile augmentation (PA). Traditionally, the PA for patients with phimosis or redundant prepuce has been a two-stage surgery-male circumcision first and hyaluronic acid (HA) injections then. We introduced a one-stage procedure for these patients to reduce the cost and recovery time.Methods Eighty-three men who received one-stage male circumcision and HA injection simultaneously in a regional hospital from January 2018 to December 2019 were retrospectively reviewed. In the male circumcision, the penile skin was sharply resected to preserve the integrity of the superficial fascia. HA was injected into the space between the superficial fascia and the deep fascia of the penis. Patients were followed up at 3 months, 6 months, and 12 months after the surgery. Penile maximal girth, patients’ satisfaction level for (using a 5-point scale, with 1 indicating very dissatisfied and 5 indicating very satisfied.) penile appearance and surgical complications were recorded during follow-up.Results Eighty-three men (aged 35.72 ± 4.73 years) received one-stage male circumcision and HA injection surgery. The dosage of HA was 16.10 ± 2.32 ml. The penile maximal girth growth was 2.33 ± 1.27 cm, 1.80 ± 1.11 cm, and 1.31 ± 0.71 cm at the 3-month, 6-month, and 12-month follow-ups, respectively. The satisfaction level for penile appearance was 2.5 ± 0.9 on the final follow-up The complications included 1 (1.2%) infection and 1 subcutaneous bleeding (1.2%). No HA leakage happened.Conclusion One-stage simultaneous male circumcision and HA injection were proven to ensure surgical efficacy and safety for men reporting a small penis with redundant prepuce or phimosis.
Diversity and pathogenic characteristics of the Fusarium species isolated from minor legumes in Korea
Authors: Ha Min Sun , Ryu Hyunjoo , Ju Ho Jong , Choi Hyo-Won 
Published: May 2023

Abstract
Several legume plants exhibiting wilt symptoms were obtained from legume plantations in Korea from 2020 to 2021. Different fungal genera, including Fusarium spp.; Colletotrichum spp., Macrophomina spp., Rhizoctonia spp., Pythium sp., Phytophthora sp., and Lasiodiplodia sp., were isolated from the internal tissues of the obtained plant roots and stems. Fusarium spp. were the most dominant, accounting for 71% (29) of the isolates. The Fusarium isolates were identified via morphological characteristics and molecular identification using the DNA sequence of translation elongation factor 1 alpha and RNA polymerase II second largest subunit regions. In the pathogenicity test, Fusarium oxysporum and Fusarium fujikuroi generally exhibited high virulence, and Fusarium falciforme, Fusarium metavorans, and Fusarium ipomoeae species exhibited low or no virulence. The NC20-738, NC20-739, and NC21-950 isolates demonstrated the widest host range as they infected all the nine crop plants. Conversely, the NC20-772 isolate induced disease only in adzuki beans and exhibited the narrowest host range. The diverse information regarding the Fusarium spp. obtained in this study will provide the basis for implementing management strategies for Fusarium-induced plant diseases to minimize economic loss associated with minor legume crops in Korea.
Adenosine deaminase family acting on RNA 1 may be a de novo target on endometriosis
Authors: Vu Thuy Ha , Nakamura Keiichiro , Shigeyasu Kunitoshi , Kubo Kotaro , Kashino Chiaki , Masuyama Hisashi 
Published: May 2023

Abstract
Adenosine deaminase family acting on RNA 1 (ADAR1) expression was examined to determine its correlation with endometriosis. The biological functions and inhibitory effects of ADAR1 knockdown were investigated in the EMC100, EMC214, and EPC-1 cell lines. ADAR1 was examined in patients with and without endometriosis using reverse transcription polymerase chain reaction (RT-PCR). The apoptotic expression of ADAR1 small interfering RNA (siRNA) was confirmed using flow cytometry. The biological functions and inhibitory effects of ADAR1 knockdown were investigated using RT-PCR in immortalized human uterine endometrial cell lines. ADAR1 expression was significantly higher in patients with endometriosis than in those without (P = 0.044). The proportions of viable cells decreased to 60.8%, 61.9%, and 72.9% (mock) and 58.9%, 40.2% and 59.1% (control) of the control cell viability at 48 hours after transient transfection of the ADAR1 siRNA into EMC100, EMC214, and EPC-1 cell lines. ADAR1 knockdown led to apoptosis through MDA-5, RIG-I, PKR, IRF3, IRF7, Caspase3, Caspase7, and Caspase8 expression. ADAR1 could be a potential therapeutic target in endometriosis.
Metabarcoding Combined with Specific Mini-barcodes Enables Qualitative and Quantitative Analysis of Traditional Chinese Medicine: A Case Study of Lonicerae japonicae flos
Authors: Gao Yuan , Zhang Xiaoying , Wang Wenxiu , Xing Zhimei , Xu Liuwei , Tian Xiaoxuan 
Published: May 2023

Abstract
BACKGROUND: Lonicerae japonicae flos, also known as Jinyinhua (JYH), is an important component of traditional Chinese patent medicine (TCPM) products. However, the potential for adulteration and substitution with low-quality materials highlights the need for a reliable and sensitive approach to identify the species composition of TCPM products for consumer safety.
METHODS AND RESULTS: We used universal ITS2 primers to amplify TCPMs containing JYH. However, the results were inconclusive, as only one operational taxonomic unit (OTU) was identified as Lonicera sp., which could not be identified at the species level. To confirm the species identification of Lonicera sp. in TCPM, we developed a short mini-barcode primer based on thepsbA-trnH region, which, in combination with DNA metabarcoding technology, allowed for qualitative and quantitative analysis of artificially mixed samples. We applied the mini-barcode to distinguish TCPMs containing JYH and demonstrated its relatively accurate quantitative ability in identifying twoLonicera species.
CONCLUSIONS: Our study presents a method for qualitative and quantitative identification of JYH, providing a promising application of DNA metabarcoding technology in the quality control of TCPM products.
Bioavailable vitamin D levels in children with first episode nephrotic syndrome: A longitudinal study.
Authors: Charan Sai , Saha Abhijeet , Dhull Rachita Singh , Nagia Anita , Goyal Rajeev , Singh Prachi , Pemde Harish Kumar 
Published: May 2023

Abstract
Background
Bioavailable vitamin D levels is could be a better marker than total 25 hydroxy vitamin D levels to assess vitamin D status in children with nephrotic syndrome.
Primary objective
To assess the levels of Serum bioavailable vitamin D in children aged 1 to 12 years with idiopathic FENS and in healthy controls.
Secondary objective
1. To measure the levels of bioavailable vitamin D in FENS and after 4 weeks of standard steroid therapy induced remission.
2. To compare levels of serum and urine VDBP in FENS and after 4 weeks of standard steroid therapy induced remission.
Materials and Methods
A longitudinal study was conducted in children between age 1 to 12 years with idiopathic first episode nephrotic syndrome. After diagnosis of nephrotic syndrome as per ISPN guidelines, additional investigations like calcium, phosphorus, ALP, 25 hydroxy vitamin D, PTH, serum Vitamin D binding protein (VDBP) and urinary VDBP. Bioavailable and free vitamin D was calculated using above data. The patients were followed up after 4 weeks of remission and investigations were repeated and bioavailable vitamin D was calculated again, later results were compared and analysed. Appropriate statistical tests were applied for parametric and non-parametric data. P value of less than 0.05 was considered statistically significant.
Results:
The mean 25 hydroxy vitamin D level was 11.27(6.08) at FENS and at 4 weeks follow-up it was 13.65 (6.24). Hence children with FENS remained deficient in 25 hydroxy vitamin D, both during relapse and remission compared to healthy controls (15.97 ng/ml). The mean serum VDBP level in FENS during relapse was 242.90 (127.75). There was significant correlation of serum VDBP with serum albumin levels (p value 0.04). At 4 weeks of remission the VDBP levels increased to 550.7(219.7), this increase was significant (p value <0.001). The mean free vitamin D at FENS was 1.54 (1.70) pg/ml, on follow-up visit at 4 weeks of remission the free vitamin D levels decreased to 0.68 (0.53) pg/ml. The mean bioavailable vitamin D in FENS during relapse was 0.75 (0.84) ng/ml and on follow-up at 4 weeks of remission increased to 1.11 (0.84) ng/ml (p value = 0.015).
Conclusion
Children with FENS are deficient of vitamin D levels. The free and bioavailable vitamin D levels are reduced in children with FENS during proteinuria, as compared to healthy controls. Further studies showing correlation of bioavailable vitamin D and 25 hydroxyvitamin D with bone mineral density are required in children to validate the usage of bioavailable vitamin D in clinical practice.
An effective technique for solving generalized Cahn-Hilliard (C-H) problems
Authors: Hassan A. , Arafa A. A. M. , Rida S. Z. , Dagher M. A. , Sherbiny Hamed M. El 
Published: May 2023

Abstract
Throughout this paper, we apply the Optimal Homotopy Asymptotic Method (OHAM) to find out the numerical solutions of the fractional Cahn-Hilliard (C-H) equation. The most advantage of this method is that it contains parameters that strongly control the solution series convergence. Approximate solutions of the C-H equation were compared with the exact solutions. A comparison of these solutions with the exact solution shows that our method is more effective and accurate for solving nonlinear differential equations.
Evaluation of age-related changes in teneligliptin pharmacokinetics in Japanese and Caucasian subjects using a physiologically based pharmacokinetic model
Authors: Iijima Hiroaki , Shimizu Hidetoshi , Mori-Anai Kazumi , Kawaguchi Atsuhiro , Mochida Yoji , Yamauchi Toshimasa , Kadowaki Takashi 
Published: May 2023

Abstract
Teneligliptin, an antidiabetic dipeptidyl peptidase-4 inhibitor, is widely used in older patients. However, the pharmacokinetic data for teneligliptin in older people are limited. Therefore, we simulated the plasma concentration–time profiles for teneligliptin at a dose of 20 mg (single and multiple doses) in virtual Japanese (20–70 years old) and Caucasian (20–98 years old) subjects using the physiologically based pharmacokinetic (PBPK) simulator Simcyp. In both populations up to 70 years old, the area under the plasma concentration–time curve (AUC) of teneligliptin tended to increase slightly with age. However, no clear age-related change in the maximum plasma concentration (Cmax) of teneligliptin was observed. In Caucasians ≥70 years old, the AUC tended to increase but the ratio of change in Cmax was smaller than that in AUC. In both populations, there were positive correlations between AUC and age, but not between Cmax and age. The results of PK simulations using the PBPK model across a wide age range, which included older subjects for whom clinical PK data are difficult to collect, suggested that there are some age-related changes in AUC of teneligliptin, and that Cmax is less affected by age than AUC.
Field Efficacy of Various Insecticides and Trichogramma Chilonis (Ishii) Against Tomato Fruit Borer Helicoverpa Armigera (Hubner) (Lepidoptera: Notctuidae)
Authors: Khan Amani Mulk , Saljoqi Ahmad-Ur-Rahman , Ullah Mehran , Amin Muhammad 
Published: May 2023

Abstract
The present study investigated the comparative efficacy of botanical extracts (Neem 2 and 3%), commercial insecticide (Radiant) and bio-agent (Trichogramma chilonis) against tomato fruit borer Helicoverpa armigera in tomato crop under field conditions, Agriculture Research Institute (ARI) Tarnab Peshawar, 2019. The experiment was laid out under randomized complete block design (RCBD) with factorial arrangement having five treatments (Radiant, Neem (2 and 3%) and Trichogramma chilonis) replicated three times. Pre spray data was recorded before 24hrs of treatment application while post spray data was recorded after different time intervals (24hrs, 48hrs, 72hrs, 5 days, 7days, 10 days and 15 days) of each treatment application. Data regarding infestation showed that highest percent reduction over control was recorded in Radiant treated plots followed by Neem 3% and Trichogramma treated plots. The efficacy of each treatment was recorded at peak after 7 days then gradually declined and was found minimum after 15 days interval. However, highest larval and eggs infestation were recorded in control plots throughout the experimental period. Statistical analysis of the data also revealed that botanical extracts (Neem 2 and 3%), commercial insecticide (Radiant) and bio agent (Trichogramma chilonis) significantly reduced percent fruit damage. Highest percent reduction in comparison with control was recorded in the plots treated with commercial insecticide Radiant. Data further revealed that each treatment application significantly increased total tomato yield and total marketable tomato yield. Highest tomato total marketable yield (9583 kg ha-1) with 80% increase over control was recorded in the Radian treated plots. It can be concluded that Radiant, Trichocards and Neem 3% comparatively have a high potential in reduction of fruit infestation which as a result gives higher yield with high percentage of marketable fruits and with highest cost benefit ratio.
Impaired thyroid hormone sensitivity increases the risk of papillary thyroid cancer and cervical lymph node metastasis
Authors: Muhanhali Dilidaer , Deng Lingxin , Ai Zhilong , Ling Yan 
Published: May 2023

Abstract
Background: The association of thyroid hormone sensitivity with papillary thyroid carcinoma (PTC) isunclear. This study investigated the relationship between the thyroid hormone sensitivity indices and the risk of PTC and the influence of thyroid hormone sensitivity on the aggressive clinicopathologic features of PTC.
Methods: This retrospective studyrecruited 1225 PTC patients and 369 patients with benign nodules undergoing surgery in Zhongshan Hospital in 2020. The thyroid hormone sensitivity indices were thyroid feedback quantile-based index (TFQI), TSH index (TSHI) and thyrotropin thyroxine resistance index (TT4RI). We employed logistic regression models to explore the correlation between the thyroid hormone sensitivity indices and the risk of PTC and its cervical lymph node metastasis (LNM).
Results: PTC patients had significantly higher levels of TSH, TFQI, TSHI and TT4RI compared to the patients with benign nodules, but thyroid hormone levels did not differ significantly between the two groups. Logistic regression analysis revealed that the higher levels of TFQI, TSHI, and TT4RI were associated with an increased risk of PTC after adjustment for multiple risk factors (TFQI: OR=1.92, 95%CI: 1.39-2.65, P<0.001; TSHI: OR=2.33, 95%CI:1.67-3.26, P<0.001; TT4RI: OR=2.41, 95%CI:1.73-3.36, P<0.001). In addition, patients with decreased thyroid hormone sensitivity had a higher risk of cervical LNM in multiple logistic regression analysis (TFQI: OR=1.38, 95%CI:1.03-1.86, P=0.03; TSHI: OR=1.37, 95%CI:1.02-1.84, P=0.04; TT4RI: OR= 1.40, 95%CI:1.05-1.89, P=0.02).
Conclusion: Impaired sensitivity to thyroid hormone was associated with an increased risk of PTC, and it is also associated with a higher risk of cervical LNM in PTC patients.