bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fund management in the interwar period: UK investment trust portfolio asset allocation in the 1920s
Authors: Sotiropoulos Dimitris P , Rutterford Janette , Tori Daniele , Kyparissis Antonis 
Published: January 2023

Abstract
This study investigates the portfolio asset allocation of UK investment trusts between 1914 and 1928 using a unique hand-collected dataset of 41 companies, which comprises 40,875 portfolio holdings. UK investment trusts not only survived WWI without major losses but also had a remarkable performance in the 1920s, which led to a wave of new incorporations. The 1920s was a period of significant shifts in investment trust portfolio composition and our analysis examines the main reasons for this. We show that investment trust managers were able to adjust quickly to the new socioeconomic circumstances and secure high profitability for their shareholders.
The science of clinical quality registries
Authors: Parker Kirsten J , Hickman Louise D , Ferguson Caleb 
Published: January 2023

AbstractClinical quality registries can be a transformational tool to improve healthcare delivery. Clinical registries with an incorporated quality emphasis identify evidence-practice gaps, inform quality improvement, and provide foundational research data to examine and improve health-related outcomes. For registries to create an impact it is essential that clinicians and researchers understand historical context, importance, advantages, and key criticisms. This methodological paper highlights the skills and capabilities required to build and maintain a robust clinical quality registry. This includes key measures to ensure data security, quality control, ongoing operational components, and benchmarking of care outcomes.
Klotho protects chromosomal DNA from radiation-induced damage
Authors: Nakayama Shinya , Sun Jiying , Horikoshi Yasunori , Kamimura Yoshitaka , Ike Takeshi , Fujino Shu , Kinugasa Yasuha , Sasaki Kensuke , Nakashima Ayumu , Masaki Takao , Tashiro Satoshi 
Published: January 2023

Abstract
Klotho is an anti-aging, single-pass transmembrane protein found mainly in the kidney. Although aging is likely to be associated with DNA damage, the involvement of Klotho in protecting cells from DNA damage is still unclear.
In this study, we examined DNA damage in human kidney cells and mouse kidney tissue after ionizing radiation (IR). The depletion and overexpression of Klotho in human kidney cells reduced and increased the cell survival rates after IR, respectively. The formation of γ-H2AX foci, representing DNA damage, was significantly elevated immediately after IR in cells with Klotho depletion and decreased in cells overexpressing Klotho. These results were confirmed in mouse renal tissues after IR. Quantification of DNA damage by a comet assay revealed that the Klotho knockdown significantly increased the amount of DNA damage immediately after IR, suggesting that Klotho protects chromosomal DNA from the induction of damage, rather than facilitating DNA repair. Consistent with this notion, Klotho was detected in both the nucleus and cytoplasm. In the nucleus, Klotho may serve to protect chromosomal DNA from damage, leading to its anti-aging effects.
Unveiling demographic and mating strategies ofPanthera oncain the Pantanal, Brazil
Authors: Fragoso Carlos Eduardo , Rampim Lilian Elaine , Quigley Howard , Buhrke Haberfeld Mario , Ayala Espíndola Wellyngton , Cabral Araújo Valquíria , Rodrigues Sartorello Leonardo , May Júnior Joares Adenilson 
Published: January 2023

AbstractWe conducted the first long-term and large-scale study of demographic characteristics and reproductive behavior in a wild jaguar (Panthera onca) population. Data were collected through a combination of direct observations and camera trapping on a study area that operates both as a cattle ranch and ecotourism destination. Jaguars exhibited two birth peaks: April/May and October/November, that are the end and the beginning of the wet season in the Pantanal, respectively. The average litter size was 1.43 ± 0.65. Single cubs made up a total of 65.7% of the births, and we found a slight predominance of females (1.15:1 ratio) in litters. The mean age at independence was 17.6 ± 0.98 months, with sex-biased dispersal, with all males (n = 27) leaving the natal home range and 63.6% of females exhibiting philopatry. The interbirth intervals were 21.8 ± 3.2 months and the mean age at first parturition was 31.8 ± 4.2 months. Our results estimated a lifetime reproductive success for female jaguars of 8.13 cubs. Our observations also indicate that female jaguars can display mating behavior during cub rearing or pregnancy, representing 41.4% of the consorts and copulations recorded. We speculate that this behavior has evolved as a defense against infanticide and physical harm to the female. To our knowledge, this is the first time that such behavior is described for this species. All aggressive interactions between females involved the presence of cubs, following the offspring–defense hypothesis, that lead to territoriality among females in mammals, regardless of food availability. In the face of growing threats to this apex predator, this work unveils several aspects of its natural history, representing a baseline for comparison with future research and providing critical information for population viability analysis and conservation planning in the long term.
Practical rationality in education: beyond the Hirst–Carr debate
Authors: Misawa Koichiro 
Published: January 2023

AbstractPaul Hirst’s philosophical ‘conversion’ from forms of knowledge to forms of social practices was largely prompted by his radical reappraisal of the philosophical underpinnings that had validated his classic conception of liberal education. The primary motivation for Hirst’s later works was to remedy his own neglect of practical reason, whose sharp distinction from theoretical reason he acknowledged he had failed to appreciate. There is much to commend in his ‘practical’ turn. The main challenge that remains, however, is that the social practices view is something of a ‘foundationalist’ two-part picture, where overwhelming priority is given to practical reason. I appreciate the importance of the two issues Hirst puts on the table, in his debate with Wilfred Carr particularly and in his later works more generally: the issues of cultivating phronesis and of making sense of the practical character of critical-reflective activities. But I argue that addressing these issues fully requires us to recognize that neither theoretical nor practical rationality can be intelligible apart from the other. To press the point home, I review the analogous debate between Hubert Dreyfus and John McDowell over whether to accept, and how to understand, the view that rational conceptuality in a relevant sense pervades not only deliberation and propositional thinking but also perception and embodied coping skills. I conclude by briefly considering the prospect that the legacy of Hirst and recent discussions around human nature, practical rationality, and the formation of reason will interactively enrich our philosophical-educational scholarship.
Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells
Authors: Shimada Mikio , Tokumiya Takumi , Miyake Tomoko , Tsukada Kaima , Kanzaki Norie , Yanagihara Hiromi , Kobayashi Junya , Matsumoto Yoshihisa 
Published: January 2023

AbstractPluripotent stem cells (PSCs) have the potential to differentiate to any of the other organs. The genome DNA integrity of PSCs is maintained by a high level of transcription for a number of genes involved in DNA repair, cell cycle and apoptosis. However, it remains unclear how high the frequency of genetic mutation is and how these DNA repair factors function in PSCs. In this study, we employed Sup F assay for the measurement of mutation frequency after UV-C irradiation in induced pluripotent stem cells (iPSCs) as PSC models and neural progenitor cells (NPCs) were derived from iPSCs as differentiated cells. iPSCs and NPCs exhibited a lower mutation frequency compared with the original skin fibroblasts. In RNA-seq analysis, iPSCs and NPCs showed a high expression of RAD18, which is involved in trans-lesion synthesis (TLS) for the emergency tolerance system during the replication process of DNA. Although RAD18 is involved in both error free and error prone TLS in somatic cells, it still remains unknown the function of RAD18 in PSCs. In this study we depleted of the RAD18 by siRNA knockdown resulted in decreased frequency of mutation in iPSCs and NPCs. Our results will provide information on the genome maintenance machinery in PSCs.
The regulatory effect of sex steroids on the RhoA/ROCK pathway in the rat distal vagina
Authors: Cellai Ilaria , Comeglio Paolo , Filippi Sandra , Martinelli Serena , Villanelli Fabio , Amore Francesca , Rapizzi Elena , Maseroli Elisa , Cipriani Sarah , Raddi Chiara , Guarnieri Giulia , Sarchielli Erica , Danza Giovanna , Morelli Annamaria , Rastrelli Giulia , Maggi Mario , Vignozzi Linda 
Published: January 2023

AbstractBackgroundSex steroids have been demonstrated as important modulators of vaginal function. The RhoA/ROCK calcium-sensitizing pathway plays a role in genital smooth muscle contractile mechanism, but its regulation has never been elucidated.AimThis study investigated the sex steroid regulation of the vaginal smooth muscle RhoA/ROCK pathway using a validated animal model.MethodsOvariectomized (OVX) Sprague-Dawley rats were treated with 17β-estradiol (E2), testosterone (T), and T with letrozole (T + L) and compared with intact animals. Contractility studies were performed to test the effect of the ROCK inhibitor Y-27632 and the nitric oxide (NO) synthase inhibitor L-NAME. In vaginal tissues, ROCK1 immunolocalization was investigated; mRNA expression was analyzed by semiquantitative reverse transcriptase–polymerase chain reaction; and RhoA membrane translocation was evaluated by Western blot. Finally, rat vaginal smooth muscle cells (rvSMCs) were isolated from the distal vagina of intact and OVX animals, and quantification of the RhoA inhibitory protein RhoGDI was performed after stimulation with NO donor sodium nitroprusside, with or without administration of the soluble guanylate cyclase inhibitor ODQ or PRKG1 inhibitor KT5823.OutcomesAndrogens are critical in inhibiting the RhoA/ROCK pathway of the smooth muscle compartment in the distal vagina.ResultsROCK1 was immunolocalized in the smooth muscle bundles and blood vessel wall of the vagina, with weak positivity detected in the epithelium. Y-27632 induced a dose-dependent relaxation of noradrenaline precontracted vaginal strips, decreased by OVX and restored by E2, while T and T + L decreased it below the OVX level. In Western blot analysis, when compared with control, OVX significantly induced RhoA activation, as revealed by its membrane translocation, with T reverting it at a level significantly lower than in controls. This effect was not exerted by E2. Abolishing NO formation via L-NAME increased Y-27632 responsiveness in the OVX + T group; L-NAME had partial effects in controls while not modulating Y-27632 responsiveness in the OVX and OVX + E2 groups. Finally, stimulation of rvSMCs from control animals with sodium nitroprusside significantly increased RhoGDI protein expression, counteracted by ODQ and partially by KT5823 incubation; no effect was observed in rvSMCs from OVX rats.Clinical ImplicationsAndrogens, by inhibiting the RhoA/ROCK pathway, could positively contribute to vaginal smooth muscle relaxation, favoring sexual intercourse.Strengths and LimitationsThis study describes the role of androgens in maintaining vaginal well-being. The absence of a sham-operated animal group and the use of the only intact animal as control represented a limitation to the study.
The contribution of the cervix to sexual response: an online survey study
Authors: Giovannetti Olivia , Tomalty Diane , Gilmore Shelby , Pattison Anne , Komisaruk Barry , Goldstein Sue , Hannan Johanna , Goldstein Irwin , Pukall Caroline , Adams Michael A 
Published: January 2023

AbstractBackgroundThe role of the cervix in sexual response has been poorly studied, despite previous research indicating that some women experience pleasurable sexual sensations from cervical stimulation; given previous reports of sexual issues after cervix electrocautery, it is possible that cervical injury may compromise the role of the cervix in sexual functioning.AimThe aims of this study were to examine locations of pleasurable sexual sensations, to identify sexual communication barriers, and to investigate if cervical procedures are associated with negative impacts on sexual function.MethodsWomen with (n = 72) and without (n = 235) a history of a gynecological procedure completed an online survey assessing demographics, medical history, sexual function (including locations of sexual pleasure and pain on diagrams), and barriers. The procedure group was divided into subgroups of those who had experienced a cervical (n = 47) or noncervical (n = 25) procedure. Chi-square analyses and t tests were conducted.OutcomesOutcomes included locations and ratings of pleasurable and painful sexual stimulation, as well as sexual function.ResultsOver 16% of participants reported experiencing some pleasurable sexual sensations from the cervix. The gynecological procedure group (n = 72) reported significantly higher pain in the vagina and lower rates of pleasure in their external genitals, vagina, deep vagina, anterior and posterior vaginal walls, and clitoris vs the non–gynecological procedure (n = 235) group. The gynecological procedure group and the cervical procedure subgroup (n = 47) reported significant decreases in desire, arousal, and lubrication and increased avoidance of sexual activity due to vaginal dryness. The gynecological procedure group reported significant pain with vaginal stimulation, whereas the cervical subgroup identified significant pain with cervical and clitoral stimulation.Clinical ImplicationsCervical stimulation elicits some pleasurable sexual sensations for many women, and gynecological procedures that affect the cervix are associated with pain and sexual issues; thus, health care providers should counsel patients about the possibility of related sexual concerns.Strengths and LimitationsThis study is the first to examine locations of pleasure and pain and experiences of sexual pleasure and function in participants who underwent a gynecological procedure. A hybrid measure was used to assess sexual issues, including symptoms of dysfunction.ConclusionResults indicate an association between cervical procedures and sexual issues, supporting the need to inform patients of this possibility following cervical procedures.
Sexual functioning beliefs, sexual satisfaction, and sexual functioning in women: a cross-sectional mediation analysis
Authors: Peixoto Maria Manuela , Lopes Júlia 
Published: January 2023

AbstractBackgroundSexual dysfunctional beliefs are known to be negative predictors of women’s sexual functioning and sexual satisfaction, but little is known about the role that beliefs about sexual functioning play in women’s sexual functioning and sexual satisfaction.AimThe purpose of the present study was to examine the mediating role of sexual functioning in the relationship between women’s beliefs about sexual functioning and sexual satisfaction.MethodsA sample of 301 women was invited to participate in a web survey about female sexuality in May 2020.OutcomesParticipants completed the sociodemographic questionnaire, the Beliefs About Sexual Functioning Scale, the Sexual Satisfaction Scale for Women, and the Female Sexual Functioning Index.ResultsSexual functioning was a significant mediator in the relationship between beliefs about sexual functioning and sexual satisfaction in women. When the dimensions of sexual functioning were considered as mediators, only sexual desire and absence of sexual pain were significant mediators in the relationship between beliefs about sexual functioning and sexual satisfaction in women.Clinical ImplicationsNegative beliefs about sexual functioning in women are associated with poor sexual functioning and lack of sexual satisfaction. Therefore, beliefs about sexual functioning should be assessed during the intervention for women’s sexual difficulties and to increase sexual pleasure and satisfaction. In addition, special attention should be paid to women with problems with sexual desire and sexual pain when beliefs about sexual functioning are being assessed.Strengths and LimitationsThis study is one of the first attempts to explore the role of beliefs about sexual functioning in women’s sexual satisfaction and to examine the mediating role of sexual functioning. Limitations include the small sample size, the lack of a clinical sample, and the nonlongitudinal methodological approach.ConclusionBeliefs about sexual functioning are a negative predictor of sexual functioning and sexual satisfaction in women. In addition, women with sexual pain and lack of sexual desire are more likely to have lower sexual satisfaction if they have more negative beliefs about sexual functioning.
The impact of growth at elevated [CO2] on stomatal anatomy and behavior differs between wheat species and cultivars
Authors: Wall Shellie , Cockram James , Vialet-Chabrand Silvere , Van Rie Jeroen , Gallé Alexander , Lawson Tracy 
Published: January 2023

Abstract
The ability of plants to respond to changes in the environment is crucial to their survival and reproductive success. The impact of increasing the atmospheric CO2 concentration (a[CO2]), mediated by behavioral and developmental responses of stomata, on crop performance remains a concern under all climate change scenarios, with potential impacts on future food security. To identify possible beneficial traits that could be exploited for future breeding, phenotypic variation in morphological traits including stomatal size and density, as well as physiological responses and, critically, the effect of growth [CO2] on these traits, was assessed in six wheat relative accessions (including Aegilops tauschii, Triticum turgidum ssp. Dicoccoides, and T. turgidum ssp. dicoccon) and five elite bread wheat T. aestivum cultivars. Exploiting a range of different species and ploidy, we identified key differences in photosynthetic capacity between elite hexaploid wheat and wheat relatives. We also report differences in the speed of stomatal responses which were found to be faster in wheat relatives than in elite cultivars, a trait that could be useful for enhanced photosynthetic carbon gain and water use efficiency. Furthermore, these traits do not all appear to be influenced by elevated [CO2], and determining the underlying genetics will be critical for future breeding programmes.
Occurrence of virulence genes and methicillin-resistance inStaphylococcus aureusisolates causing subclinical bovine mastitis in Tiaret area, Algeria
Authors: Bouzidi Said , Bourabah Akila , Cheriet Sarah , Abbassi Mohamed Salah , Meliani Samia , Bouzidi Hanane 
Published: January 2023

AbstractMastitis remains the most frequent and the most expensive disease of dairy breeding. The objective of the study was to study S. aureus isolates collected from subclinical bovine mastitis in the Tiaret region, Algeria, by determining their antimicrobial susceptibility and their virulence traits. Sixty-two S. aureus isolates collected from subclinical bovine mastitis were studied by determining their antimicrobial susceptibility according to CLSI guidelines, and nine genes encoding virulence factors and resistance to methicillin and penicillin were determined by PCR. Multi-drug resistance was observed in 19 (30.64%) isolates and five (8%) were methicillin-resistant S. aureus (MRSA), four of them harbored the mecA gene; however, the mecC gene was not detected. Out of 59 penicillin-resistant isolates, 14 harbored the blaZ gene; one of them co-harbored the mecA gene. The following virulence genes were detected: eta (n = 23; 37%), icaA (20; 32.2%), icaD (18; 29%), etb (16; 25.8%), luk E-D (14; 22.5%), and sea (6; 9.6%). The tsst-1, lukF/lukS, and luk-M genes were not detected. The occurrence of MRSA and multi-drug resistant (MDR) S. aureus isolates as well as genes encoding virulence factors playing an important role in the pathogenesis of subclinical bovine mastitis and of harmful potential to human is a cause for concern.
Eccentricity or spin precession? Distinguishing subdominant effects in gravitational-wave data
Authors: Romero-Shaw Isobel M , Gerosa Davide , Loutrel Nicholas 
Published: January 2023

ABSTRACT
Eccentricity and spin precession are key observables in gravitational-wave astronomy, encoding precious information about the astrophysical formation of compact binaries together with fine details of the relativistic two-body problem. However, the two effects can mimic each other in the emitted signals, raising issues around their distinguishability. Since inferring the existence of both eccentricity and spin precession simultaneously is – at present – not possible, current state-of-the-art analyses assume that either one of the effects may be present in the data. In such a setup, what are the conditions required for a confident identification of either effect? We present simulated parameter inference studies in realistic LIGO/Virgo noise, studying events consistent with either spin precessing or eccentric binary black hole coalescences and recovering under the assumption that either of the two effects may be at play. We quantify how the distinguishability of eccentricity and spin precession increases with the number of visible orbital cycles, confirming that the signal must be sufficiently long for the two effects to be separable. The threshold depends on the injected source, with inclination, eccentricity, and effective spin playing crucial roles. In particular, for injections similar to GW190521, we find that it is impossible to confidently distinguish eccentricity from spin precession.
The Tucana dwarf spheroidal: a distant backsplash galaxy of M31?
Authors: Santos-Santos Isabel M E , Navarro Julio F , McConnachie Alan 
Published: January 2023

ABSTRACT
We use the APOSTLE Local Group (LG) cosmological hydrosimulations to examine the properties of ‘backsplash’ galaxies, i.e. dwarfs that were within the virial boundaries of the Milky Way (MW) or M31 in the past, but are today outside their virial radius (r200). More than half of all dwarfs between $1\text{ and }2\, r_{200}$ of each primary are backsplash. More distant backsplash systems, i.e. those reaching distances well beyond $2\, r_{200}$, are typically close to apocentre of nearly radial orbits, and, therefore, essentially at rest relative to their primary. We use this result to investigate which LG dwarfs beyond ∼500 kpc of either primary could be a distant backsplash satellite of MW or M31. Tucana dSph, one of the few known quiescent LG field dwarfs, at dM31 ≈ 1350 kpc and dMW ≈ 880 kpc, is a promising candidate. Tucana’s radial velocity is consistent with being at rest relative to M31. Further, Tucana is located close to M33’s orbital plane around M31, and simple orbit integrations indicate that Tucana may have been ejected during an early pericentric passage of M33 ∼11 Gyr ago, a timing that approximately coincides with Tucana’s last episode of star formation. We suggest that Tucana may have been an early-infalling satellite of M31 or M33, providing a compelling explanation for its puzzling lack of gas and ongoing star formation despite its isolated nature. In this scenario, M33 should have completed some orbits around M31, a result that may help to explain the relative dearth of M33 satellite candidates identified so far.