bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening of the differentially expressed proteins in malignant transformation of BEAS-2B cells induced by coal tar pitch extract
Authors: Chai Yaru , Ji Penghui , Effah Clement Yaw , Duan Shuyin , Feng Feifei , Wu Yongjun 
Published: March 2023

Abstract
Coal tar pitch extract (CTPE) was carcinogenic and could cause occupational lung cancer. Hence, we explored the changes of protein molecules during CTPE-induced malignant transformation (MT) of immortalized human bronchial epithelial (BEAS-2B) cells and provided clues for screening early biomarkers of CTPE-associated occupational lung cancer. The MT model of BEAS-2B cells induced by CTPE with 15.0 μg/mL. Subsequently, the MT of the BEAS-2B cells was verified by morphological observation, cell proliferation test, plate colony formation assay, and cell cycle assay. At the end of the experiment, we explored the differentially expressed proteins (DEPs) by total protein tandem mass tags quantitative proteomics technique between DMSO40 cells and CTPE40 cells. It was found that the proliferation ability, and colony formation rate were enhanced, and the cell cycle was changed. Then, bioinformatics analysis showed that a total of 107 DEPs were screened between CTPE40 and DMSO40 cells, of which 74 were up-regulated and 33 were down-regulated. As a result, 6 hub proteins were screened by protein–protein interaction network analysis. The expression levels of COX7A2, COX7C, MT-CO2, NDUFB4, and NDUFB7 were up-regulated as well as the expression of RPS29 protein was down-regulated. In summary, we established an MT model in vitro and explored the changes in protein molecules. As a result, this study suggested that changes of protein molecules, including COX7A2, COX7C, NDUFB7, MT-CO2, NDUFB4, and RPS29, occurred at the stage of BEAS-2B cell malignancy following CTPE exposure, which provided key information for screening biomarkers for CTPE-related occupational lung cancer.
Termination for breach: the prospects of the Unidroit Principles of International Commercial Contracts to interpret and supplement the Indian law of contract
Authors: Khanderia Saloni 
Published: March 2023

Abstract
Predicated on English law, termination for the breach of contract in India is regulated by the Indian Contract Act, 1872 and the Sale of Goods Act, 1930. However, these legislations do not adopt uniform criteria while stipulating the circumstances in which the parties may exercise their right to rescind the contract for breach. The complex nature of the rules on termination for breach has rendered the Indian law of contract unsuitable to govern domestic or transnational agreements. This article examines the plausible role of the International Institute for the Unification of Private Law’s (Unidroit) Principles of International Commercial Contracts (PICC) in developing the Indian law of contract. It argues in favour of a complete overhaul of the legal principles on which the parties’ right to rescind a contract for breach is based on the ground that these reflect a pro-termination instead of a favor contractus approach. Consequently, the author urges Indian lawmakers to consider employing the provisions of the PICC on the subject as a model to develop the law of the Republic to foster international trade and facilitate justice among litigants. Although an amendment to the Indian law of contract would be ideal, the judiciary should, in the meantime, refer to the PICC while adjudicating disputes arising from the breach of contracts to interpret and supplement the Indian law according to well-defined and internationally accepted standards.
Quantifying the value of viral genomics when inferring who infected whom in the 2014–16 Ebola virus outbreak in Guinea
Authors: Robert Alexis , Tsui Lok Hei Joseph , Watson Conall H , Gsell Pierre-Stéphane , Hall Yper , Rambaut Andrew , Longini Ira M , Sakoba Keïta , Kucharski Adam J , Touré Alhassane , Danmadji Nadlaou Sévérine , Saidou Barry Mamadou , Fofana Thierno Oumar , Lansana Kaba Ibrahima , Sylla Lansana , Diaby Mohamed Lamine , Soumah Ousmane , Diallo Abdourahime , Niare Amadou , Diallo Abdourahamane , Eggo Rosalind M , Caroll Miles W , Henao-Restrepo Ana Maria , Edmunds W John , Hué Stéphane 
Published: March 2023

AbstractTransmission trees can be established through detailed contact histories, statistical or phylogenetic inference, or a combination of methods. Each approach has its limitations, and the extent to which they succeed in revealing a ‘true’ transmission history remains unclear. In this study, we compared the transmission trees obtained through contact tracing investigations and various inference methods to identify the contribution and value of each approach. We studied eighty-six sequenced cases reported in Guinea between March and November 2015. Contact tracing investigations classified these cases into eight independent transmission chains. We inferred the transmission history from the genetic sequences of the cases (phylogenetic approach), their onset date (epidemiological approach), and a combination of both (combined approach). The inferred transmission trees were then compared to those from the contact tracing investigations. Inference methods using individual data sources (i.e. the phylogenetic analysis and the epidemiological approach) were insufficiently informative to accurately reconstruct the transmission trees and the direction of transmission. The combined approach was able to identify a reduced pool of infectors for each case and highlight likely connections among chains classified as independent by the contact tracing investigations. Overall, the transmissions identified by the contact tracing investigations agreed with the evolutionary history of the viral genomes, even though some cases appeared to be misclassified. Therefore, collecting genetic sequences during outbreak is key to supplement the information contained in contact tracing investigations. Although none of the methods we used could identify one unique infector per case, the combined approach highlighted the added value of mixing epidemiological and genetic information to reconstruct who infected whom.
Newly Isolated Trichoderma spp. Show Multifaceted Biocontrol Strategies to Inhibit Potato Late Blight Causal Agent Phytophthora infestans both In Vitro and In Planta
Authors: Alfiky Alsayed , Abou-Mansour Eliane , De Vrieze Mout , L'Haridon Floriane , Weisskopf Laure 
Published: March 2023

Potato growers worldwide have been at war for more than 150 years with an enemy whose lifecycle, genome size and architecture, infection rate, and economic impacts are the epitome of a plant pathogen. Phytophthora infestans is an oomycete that causes the notorious late blight infection in potato and tomato fields. This study explored the benefits of the multitalented plant symbiotic fungi Trichoderma spp. and their metabolites as potential biopesticides against P. infestans. Eleven strains of Trichoderma spp. were obtained from soil and tree barks and were identified using DNA sequence analysis of three molecular markers. The antagonistic potential of the strains against P. infestans was first evaluated in vitro. In dual-culture assays, P. infestans growth was significantly inhibited (53 to 95%) by different Trichoderma spp. through direct mycoparasitism, competition for space and nutrients, or antibiosis. The cell-free filtrates (CFFs) of different Trichoderma strains were obtained and characterized for anti- Phytophthora activities as well as biochemical stability. The obtained results indicated that Trichoderma CFFs were chemically stable and strongly decreased P. infestans’ mycelial growth and zoospore motility and viability. Similarly, in leaf-disk assays, Trichoderma CFFs showed significant protection against P. infestans infection. Ultraperformance liquid chromatography analysis revealed the presence of harzianic acid, iso-harzianic acid, and 6-pentyl-2H-pyran-2-one as major compounds in different Trichoderma CFFs. Furthermore, selected Trichoderma strains significantly protected potato plants against soil-mediated late blight infection. Finally, Trichoderma spp. showed high compatibility with a copper-based fungicide, especially at lower concentrations, suggesting that both protective agents could be combined in integrated pest management programs. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Development of a Rapid Phenotyping Method to Screen for Didymella arachidicola Resistance in Peanuts
Authors: Wood Shona E. , Poudel Barsha , Wright Graeme C. , Dieters Mark I. , Pandey Manish K. , Ash Gavin 
Published: March 2023

Net or web blotch (NB), caused by Didymella arachidicola, is an emerging threat causing a significant reduction in yield of peanuts in cooler production regions globally, including Australia, China, and Southern Africa. Host resistance against NB is poorly understood, with field screenings reliant on favorable climatic conditions. A rapid and effective phenotyping method is essential to differentiate between resistant and susceptible breeding lines and improve selection accuracy. In this study, a greenhouse screening method using mycelium as the inoculum to determine host response to D. arachidicola was developed. A liquid-culture mycelia suspension of the pathogen was sprayed onto 9-week-old peanut plants, and one disease assessment was made between 14 and 17 days after inoculation. Lesion growth in the greenhouse was found to correlate with disease response assessed under field conditions ( r = 89%). The phenotyping method developed in this study can facilitate the identification of NB resistance lines and assist in identifying regions of genetic resistance within the peanut host. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Within-Session Test-Retest Reliability of Pressure Pain Threshold and Mechanical Temporal Summation in Chronic Low Back Pain
Authors: de Oliveira Fábio Carlos Lucas , Cossette Camille , Mailloux Catherine , Wideman Timothy H. , Beaulieu Louis-David , Massé-Alarie Hugo 
Published: March 2023

Objectives:
To determine the absolute and relative within-session test-retest reliability of pressure pain threshold (PPT) and temporal summation of pain (TSP) at the low back and the forearm in individuals with chronic low back pain (CLBP) and to test the impact of different sequences of measurements on reliability metrics.

Materials and Methods:
Twenty-eight adults with CLBP were recruited. Relative (intraclass correlation coefficient [ICC] and coefficient of variation) and absolute reliability (standard error of measurement and minimal detectable changes) were quantified at 4 sites (back: sacrum and lumbar erector spinae; wrist: hand dorsum and wrist flexors) for PPT and 2 sites (hand and low back) for TSP, for various sequences of measurements.

Results:
Systematic differences were found between within test and retest for most PPT sequences at the lumbar erector spinae site and 1 TSP sequence (1-2-3) at back and hand sites, precluding reliability analyses for these data. Within-session PPT relative reliability was excellent at low back (ICC = 0.83 to 0.94) and wrist (ICC = 0.88 to 0.97) sites, whereas TSP showed good to excellent reliability at hand (ICC = 0.80 to 0.90) and low back (ICC = 0.73 to 0.89). In general, 2 and 3 measurements optimized absolute and relative reliability for TSP and PPT, respectively.

Discussion:
Within-session reliability was generally excellent for PPT and TSP at the low back and hand sites among individuals with CLBP. We recommend using 3 measurements for PPT and 2 for TSP to optimize reliability. Caution is recommended when testing PPT of the painful lower back area since a systematic difference was present between the test and retest.
Integration of collaborative care model ameliorates population level COVID-19 Pandemic-related depressive symptoms among orthopaedic clinic patient population in US major metropolitan area
Authors: Lindsey Caryn , Dornan Grant J. , McKelvey Karma 
Published: March 2023

Background:
In the United States, the COVID-19 pandemic resulted in a widespread mental health crisis. Overarchingly, medical clinics have been slow to adopt an interdisciplinary approach to patient care, though depression has been shown to negatively affect many clinical outcomes. With an ongoing, worsening mental health crisis in conjunction with a dramatic increase in demand for Total Joint Replacement anticipated by 2030, more routine depression screening and adequate mental health support is imperative. Our clinic implemented a Collaborative Care Model (CCM) in March of 2020 with a Licensed Clinical Social Worker (LCSW).

Methods:
Using data collected at our outpatient clinic between September 1, 2018 and August 31, 2021, we compared changes in Patient-Reported Outcome Measurements Information System (PROMIS) depression scores from the group of patients seen before CCM adoption (“pre-CCM”) with scores from the group after (“post-CCM”).

Results:
We found no between-group differences in PROMIS depression score changes. Increased pain interference was positively associated with increased depression in the pre-CCM group, but not in the post-CCM group.

Conclusions:
Our CCM was successful in mitigating for our patients the mental health crisis reflected among the general population during the lockdowns and uncertainty of the COVID-19 pandemic. The CCM also diminished further exacerbation of depression secondary to increased pain interference and the overall impact the pandemic had on healthcare operations. A CCM inclusive of an LCSW may also further support community resource linkage, complex care coordination, and assessment of other mental health conditions related to orthopaedic conditions or injury, such as anxiety and post-traumatic stress disorder.
The Effect of Epiphysiodesis on the Longitudinal Bone Growth of Hands or Feet in Children With Macrodactyly Based on Long-term Quantitative Analysis
Authors: Woo Soo Jin , Jung Jae Hoon , Choi Jun Ho , Kim Yumin , Kwon Sung Tack , Kim Byung Jun 
Published: March 2023

Background:
Treatment protocols for macrodactyly have not been elucidated due to its rarity and variety of clinical manifestations. This study aims to share our long-term clinical results of epiphysiodesis in children with macrodactyly.

Methods:
A retrospective chart review was performed for 17 patients with isolated macrodactyly treated with epiphysiodesis over 20 years. Length and width of each phalanx in both the affected finger and the corresponding unaffected finger in the contralateral hand were measured. Results were presented in ratios of the affected to unaffected side for each phalanx. Measuring of length and width of phalanx was performed preoperatively and postoperatively at 6, 12, and 24 months, and the last follow-up session. Postoperative satisfaction scoring was done with visual analogue scale.

Results:
The mean follow-up period was 7 years and 2 months. In the proximal phalanx, length ratio significantly decreased compared with preoperative state at after more than 24 months, in the middle phalanx after 6 months, in the distal phalanx after 12 months. When classified by the growth patterns, the progressive type showed significant decrease in length ratio at after 6 months, and the static type after 12 months. Patients were overall satisfied with the results.

Conclusion:
Epiphysiodesis effectively regulated longitudinal growth with different degree of control for different phalanges in the long-term follow-up.
Menopausal hormone therapy and the risk of type 2 diabetes mellitus: Health Insurance Database in South Korea–based retrospective cohort study
Authors: Yuk Jin-Sung , Kim Jung Min 
Published: March 2023

Abstract

Objective
Menopausal hormone therapy (MHT) is known to reduce the incidence of type 2 diabetes mellitus (T2DM); however, since the Women's Health Initiative study, the types and doses of female hormones used for MHT have changed considerably. Therefore, this study was conducted to determine whether MHT, which is currently widely prescribed, increases the risk of T2DM.

Method
We performed a retrospective cohort study based on national health insurance data and cancer screening data from 2002 to 2019. We included the MHT group as postmenopausal women older than 40 years who used at least one MHT for at least 6 months between 2003 and 2011. We subclassified the MHT group into five categories; tibolone, combined estrogen plus progestin by the manufacturer (CEPM), oral estrogen, combined estrogen plus progestin by the physician (CEPP), and transdermal estrogen. We selected the non-MHT group as postmenopausal women who had never been prescribed MHT from 2002 to 2019. We compared the incidence of T2DM between the MHT group and the non-MHT group.

Results
We enrolled 330,771 women in the MHT group and 798,550 women in the control group. T2DM was diagnosed in 15.2% of the non-MHT group, 16.6% of the tibolone group, 12.1% of the CEPM group, 16.6% of the oral estrogen group, 15.4% of the CEPP group, and 17% of the transdermal estrogen group. In Cox proportional hazard analysis adjusted for variable factors, tibolone, oral estrogen, CEPP, and transdermal estrogen increased the incidence of T2DM. In contrast, there was no change in the risk of T2DM in the CEPM group.

Conclusions
MHT, including tibolone, which is currently the most prescribed agent, increased the risk of T2DM; however, CEPM did not increase the risk of T2DM. Only tibolone increased the risk of T2DM in participants older than 70 years.
Appendicular Skeletal Muscle Mass Prediction in People Living With HIV: A Cross-sectional Study
Authors: dos Santos André Pereira , Navarro Anderson Marliere , da Silva Leonardo Santos Lopes , Erlandson Kristine M. , Webel Allison R. , Oliveira Vitor , Abdalla Pedro Pugliesi , Alves Thiago Cândido , Tasinafo Júnior Márcio Fernando , Venturini Ana Cláudia Rossini , Gomide Euripedes Barsanulfo Gonçalves , Correa Cordeiro Jéssica Fernanda , de Andrade Denise , Machado Dalmo R. L. 
Published: March 2023

Abstract
People living with HIV (PWH) experience an accelerated aging process. There is no anthropometric predictive model for appendicular skeletal muscle mass (ASM) in PWH. This study develops anthropometric models to predict and validate ASM measured by dual energy x-ray absorptiometry (DXA) in PWH; DXA scans were obtained for 125 PWH (male = 74; age >18 years) on antiretroviral therapy. Fat mass ratio was used for lipodystrophy diagnosis. A multiple stepwise linear regression considered ASMDXA as the dependent variable and validated by PRESS method. A high power of determination and low standard estimate error were found for ASMDXA-predicted (adjusted r
2 = 0.84 to 0.87, standard estimate error = 1.7–1.6 kg) and high PRESS validation coefficients (Q2
PRESS = 0.84–0.86, SPRESS = 1.7–1.6 kg). The variables included were lipodystrophy diagnosis, medial calf circumference, sex, and total body weight. We present novel, reliable, and validated anthropometric models to predict ASMDXA in PWH.
Optimization of Bayesian penalized likelihood reconstruction for 68Ga-prostate-specific membrane antigen-11 PET/computed tomography
Authors: Tang Charlene Yu Lin , Lim Gabriel K.Y. , Chua Wei Ming , Ng Cherie Wei Qi , Koo Si Xuan , Goh Charles Xian-Yang , Thang Sue Ping , Zaheer Sumbul , Lam Winnie Wing Chuen , Huang Hian Liang 
Published: March 2023

Objective
The objective of this study is to determine the optimal β value for clinical use in digital 68Ga-prostate-specific membrane antigen (PSMA-11) PET/computed tomography (CT) imaging.

Methods

68Ga PSMA PET/CT of 21 patients with prostate cancer were reconstructed using block-sequential regularized expectation maximization (β value of 400–1600) and ordered subsets expectation maximization. Nine independent blinded readers evaluated each reconstruction for overall image quality, noise level and lesion detectability. Maximum standardized uptake value (SUVmax) of the most intense lesion, liver SUVmean and liver SUVSD were recorded. Lesions were then subdivided according to uptake and size; the SUVmax of these lesions were analyzed.

Results
There is a statistically significant correlation between improvement in image quality and β value, with the best being β 1400. This trend was also seen in image noise (P < 0.001), with the least image noise reported with β 1400. Lesion detectability was not significantly different between the different β values (P =  0.6452). There was no statistically significant difference in SUVmax of the most intense lesion (P = 0.9966) and SUVmean of liver background between the different β values (P = 0.9999); however, the SUVSD of the liver background showed a clear trend, with the lowest with β 1400 (P = 0.0008). There was a decreasing trend observed in SUVmax when β values increased from 800 to 1400 for all four subgroups, and this decrease was greatest in small and low uptake lesions.

Conclusion
Bayesian penalized likelihood reconstruction algorithms improve image quality without affecting lesion detectability. A β value of 1400 is optimal.
Vertebral Compression Fractures in Very Early Onset Inflammatory Bowel Disease
Authors: Collen Lauren V. , Snapper Scott B. , Gordon Rebecca J. 
Published: March 2023

Objectives:
To describe clinical characteristics and outcomes of 3 patients with very early onset inflammatory bowel disease (VEOIBD) and vertebral compression fractures.

Methods:
Patients with VEOIBD receiving care at a single tertiary center were prospectively enrolled in a longitudinal data repository. Retrospective chart review was performed to identify clinical characteristics and comorbidities. Those with clinically apparent vertebral compression fractures subsequently underwent an additional chart review focused on bone health.

Results:
Three out of 216 (1.4%) patients with VEOIBD had symptomatic vertebral compression fractures. Of the 3 patients with vertebral compression fractures, all had Crohn’s disease, 2 had monogenic inflammatory bowel disease, and all reported back pain. One patient notably had a normal dual-energy X-ray absorptiometry, highlighting a potential limitation of dual-energy X-ray absorptiometry to identify increased skeletal fragility in this population. Risk factors for suboptimal bone health included chronic inflammation secondary to poorly controlled inflammatory bowel disease, substantial glucocorticoid exposure, chronic use of other medications associated with suboptimal bone health including proton pump inhibitors and granulocyte colony-stimulating factor, and solid organ transplant. Patients treated with bisphosphonates had improved clinical outcomes, with resolution of back pain and increased bone mineral density.

Conclusions:
Vertebral compression fracture should be considered in the differential diagnosis of patients with VEOIBD and back pain, especially in those with other risk factors for suboptimal bone health. Treatment of compression fractures with bisphosphonates resulted in resolution of back pain and improved bone density.