bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of a new protocol strategy to control the ectoparasitic infestation in Nile tilapia (Oreochromis niloticus) using efficient natural products
Authors: Hanna Magdy I. , EL Sayed Aya T. , El Megeed Ola Hasan Abd , Ibrahim Marwa A. , Korany Reda M. S. , Attia Marwa M. 
Published: January 2025

AbstractThis study aimed to evaluate alternative in vivo treatment trials using natural products for ectoparasitic infestation on Nile tilapia; these two products were not previously used in the treatment of parasitic fish diseases. So, a total of 400 Oreochromis niloticus (O. niloticus) fish measured 10–15 cm in length; 350 from a fish farm in (Kafr Elsheikh and 50 from Nile River (Al Bahr Al Aazam), Egypt. The examined fishes were 10–15 ± 0.5 cm long and weighed from 45 g ± 5. The collected fish were examined for different clinical abnormalities. Each part of the fish underwent a careful microscopic examination of mucous surrounding the skin; gills and fins. Two feed supplements were used experimentally to decrease mortality and treat fish against ectoparasites (Herb-All PARA-X® and Herb-All CALM®). Total mRNA was extracted from the gills of different examined groups. Glucose; nitric oxide; cortisol as well as lysozyme activity were assessed in all groups. The gills of the examined fish were collected for histopathological examination. Only, Dactyolgyrus sp. was recovered. The intensity of the parasite was counted per microscopic field. The treated groups showed low levels of the parameters compared to the control positive group. Up-regulation of both Tumor necrosis factor-α (TNF- α) and heat shock protein 70 (hsp-70) were detected in fins, gills, and skin in the infested tilapia. The treatment and prophylaxis significantly downregulated both genes in the studied organ in a dose-dependent manner. Recorded lesions which were scored according to their severity. In conclusion; following the use of those products, fish health has been greatly improved and that is indicated by findings of immune reactions as well as histopathology.
Enhancing newborn screening sensitivity and specificity for missed NICCD using selected amino acids and acylcarnitines
Authors: Wang Peiyao , Hu Lingwei , Chen Yuhe , Zhou Duo , Zhu Shasha , Zhang Ting , Cen Ziyan , He Qimin , Wu Benqing , Huang Xinwen 
Published: January 2025

Abstract
Purpose
To enhance the detection rate of Neonatal Intrahepatic Cholestasis caused by Citrin Deficiency (NICCD) through newborn screening (NBS), we analyzed the metabolic profiles of missed patients and proposed a more reliable method for early diagnosis.

Methods
In this retrospective study, NICCD patients were classified into “Newborn Screening” (64 individuals) and “Missed Screening” (52 individuals) groups. Metabolic profiles were analyzed using the non-derivatized MS/MS Kit, and genetic mutations were identified via next-generation sequencing and confirmed by Sanger sequencing. Receiver Operating Characteristic (ROC) analysis evaluated the predictive value of amino acids and acylcarnitines in dried blood spots (DBS) for identifying missed patients including 40 missed patients and 17,269 healthy individuals, with additional validation using 12 missed patients and 454 healthy controls.

Results
The age of diagnosis was significantly higher in the “Missed Screening” group compared to the “Newborn Screening” group (74.50 vs. 18.00 days, P < 0.001). ROC analysis revealed that citrulline had excellent diagnostic accuracy for missed patients, with an AUC of 0.970 and a cut-off value of 17.57 µmol/L. Additionally, glycine, phenylalanine, ornithine, and C8 were significant markers, each with an AUC greater than 0.70. A combination of these markers achieved an AUC of 0.996 with a cut-off value of 0.00195. Validation demonstrated a true positive rate of 91.67% and a true negative rate of 96.48%. Common SLC25A13 mutations in both groups were c.852_855del, IVS16ins3kb, and c.615 + 5G > A.

Conclusions
Combining multiple metabolic markers during NBS significantly improves sensitivity and specificity for detecting missed NICCD cases. However, the relationship between genetic mutations and missed cases remains unclear.
Clinician perspectives on designing and implementing a hereditary cancer transition clinic
Authors: Gabriel Jazmine L. , Schlieder Victoria , Goehringer Jessica M. , Leitzel Tracey , Sugrue Emily Ann , Zultevicz Sarah , Davis Thomas W. , Campbell-Salome Gemme , Romagnoli Katrina 
Published: January 2025

AbstractEarly identification of hereditary cancer predisposition in adolescents and young adults represents a unique opportunity to target cancer prevention and improve survival in a population at risk for adverse health outcomes. However, adolescents and young adults face challenges unique to their stage of life that can undermine their transition from pediatric to adult healthcare and lead to interruptions in preventative care. The purpose of this study was to understand expert perspectives on factors relevant to designing and implementing a transition clinic for adolescents and young adults with hereditary cancer predisposition. We used qualitative methods informed by human-centered design and implementation science to identify implementation considerations rooted in clinician experience. To understand clinic design and clinician experience at Geisinger transition clinics, we conducted a contextual inquiry using clinic observations and follow-up interviews of clinicians. To learn about designing and implementing a transition program, we also conducted in-depth interviews with national transition experts actively involved in developing, implementing, or participating in transition clinics around the United States. The contextual inquiry resulted in three diagrams depicting the following common elements of transition clinics at our institution: relationship building with patients, care coordination, stepwise transition education, communication between providers, and a sustainable clinic home. Interviews were analyzed deductively using thematic analysis to learn clinician perspectives about program implementation specific to each domain of the RE-AIM theoretical framework: reach, effectiveness, adoption, implementation, and maintenance.
Effects of Trichinella spiralis and its serine protease inhibitors on intestinal mucosal barrier function
Authors: Wang Ruibiao , Zhang Yuheng , Li Zhixin , Zhen Jingbo , Li Qiankun , Zhang Qi , Yang Yuqi , Liu Xueting , Lu Yixin 
Published: January 2025

AbstractTrichinella spiralis (T. spiralis) is a highly pathogenic zoonotic nematode that poses significant public health risks and causes substantial economic losses. Understanding its invasion mechanisms is crucial. This study explored how the serine protease inhibitors (SPIs) secreted by T. spiralis affect the host’s intestinal epithelial barrier. Furthermore, the effects of T. spiralis infection on the jejunal barrier function in mice were investigated. The histopathological analysis indicated significant damage to the jejunum, which peaked at day 7 post-infection (dpi). The results of RT-qPCR and western blotting revealed marked reductions in tight junction proteins (ZO-1, occludin, claudin-3), mucins (MUC-1, MUC-2), and anti-inflammatory cytokines (TGF-β, IL-10) from 1 to 15 dpi. There was also increased expression of Toll-like receptors (TLR-1, TLR-2, TLR-4) and pro-inflammatory cytokines (TNF-α, IL-1β). Recombinant SPIs (rKaSPI, rAdSPI) were purified, co-cultured with intestinal epithelial cells (IPECs), and used in mouse models. The protein expression changes in IPECs and mice were consistent with those in T. spiralis-infected tissues. Both SPIs caused the down-regulation of ZO-1, occludin, claudin-3, MUC-1, MUC-2, TGF-β, and IL-10 while up-regulating TLR-4 and pro-inflammatory cytokines. As a result, the intestinal barrier was disrupted, and inflammation was exacerbated. Notably, rAdSPI had a more pronounced effect. In summary, T. spiralis infection caused significant jejunal damage and disrupted the intestinal barrier. T. spiralis-secreted SPIs, especially serpin-type serine protease inhibitors (AdSPI), were pivotal in facilitating invasion by compromising the host’s intestinal barrier and promoting inflammation. This study provides insights into T. spiralis invasion mechanisms and the potential targets for trichinellosis prevention and control.
Microbial pesticides: a bibliometric analysis of global research trends (1973–2024)
Authors: Sawangproh Weerachon , Paejaroen Paiphan , Afifah Lutfi , Phaenark Chetsada 
Published: January 2025

Abstract
Background
Microbial pesticides, derived from microorganisms such as bacteria, fungi, and viruses, present sustainable alternatives to chemical pesticides, thereby promoting environmentally friendly agricultural practices. This bibliometric analysis examines the evolution of microbial pesticide research from 1973 to October 2024, utilizing the Scopus database to identify trends, geographic distribution, collaboration networks, and key research areas.

Results
A total of 247 articles were analyzed, revealing an annual growth rate of 5.16%, with a significant increase in publications over recent decades. This upward trend indicates a shift towards ecologically conscious pest management. China leads in research output with 341 publications, followed by USA (227) and India (126), highlighting strong international collaboration, particularly between China and USA, where many publications are co-authored by researchers from multiple countries. The primary research areas include Agricultural and Biological Sciences, alongside substantial contributions from Environmental Science and Immunology. The analysis identified thematic clusters, emphasizing key microbial agents such as Bacillus thuringiensis for pest control agent. Key journals, including Biocontrol Science and Technology, Journal of Biopesticides, and Journal of Invertebrate Pathology, serve as essential platforms for disseminating these findings.

Conclusion
The findings reflect an increasing recognition of microbial pesticides in integrated pest management, aligning with global trends toward sustainable agricultural practices and food safety by reducing reliance on chemical pesticides. Contributions from institutions such as Fujian Agriculture and Forestry University and Guizhou University in China further highlight the academic support driving advancements in this field.
Safety of accessing brachial veins for large-bore upper extremity venous thrombectomy using ClotTriever Thrombectomy System
Authors: Verst Luke A. , Greenberg Colvin , Shin David S. , Abad-Santos Matthew , Monroe Eric J. , Makary Mina S. , Chick Jeffrey Forris Beecham 
Published: January 2025

Abstract
Purpose
To evaluate access site adverse events following ClotTriever-mediated large-bore mechanical thrombectomy via small upper extremity deep veins (< 6-mm).

Materials and methods
Twenty patients, including 24 upper extremity venous access sites, underwent ClotTriever-mediated large-bore thrombectomy of the upper extremity and thoracic central veins for symptomatic deep vein obstruction unresponsive to anticoagulation. Patients without follow-up venous duplex examinations (n = 3) were excluded. Patients who had > 6-mm diameter veins accessed (n = 3) were excluded. Temporary purse-string sutures and manual pressure were used for access site hemostasis in all patients. Vein access site and diameter, technical success (defined as placement of the 13.5-French ClotTriever sheath followed by thrombectomy), and early (< 30-days) and late (> 30-days) access site-related adverse events (according to the Adverse Event Classification by the Society of Interventional Radiology criteria) were recorded.

Results
Fourteen patients (8 males, 6 females; mean age 51.7 ± 13.6 years) comprising 16 upper extremity venous access sites were included in this study. Access sites included: right brachial (n = 7), left brachial (n = 5), and bilateral brachial (n = 2) veins. The mean access site diameter was 4.3-mm ± 0.67-mm. Technical success was achieved via all access sites. Six (42.9%) patients underwent stent reconstruction following thrombectomy through the same accesses. After the procedure, all purse-string sutures were removed within 24 h. Three (21.4%) patients experienced small access site hematomas that did not require transfusion, intervention, or prolonged hospitalization. Initial follow-up venous duplex ultrasounds were performed at 29.3 ± 21.7 days following intervention. The mean follow-up interval to the second and third venous duplex ultrasounds were 124.3 ± 64-days and 225.1 ± 80.1 days, respectively. One (7.1%) patient developed right arm swelling six days after the procedure and was found to have thrombosis of the previously accessed right brachial vein. No other clinically or sonographically significant access site adverse events were observed.

Conclusion
ClotTriever-mediated large-bore thrombectomy via small upper extremity veins is safe with minimal access site adverse events.
Injectable Estradiol Use in Transgender and Gender-Diverse Individuals throughout the United States
Authors: Misakian Aaron L , Kelley Carly E , Sullivan Erika A , Chang Julia J , Singh Gagandeep , Kokosa Sarah , Avila Jonathan , Cooper Holly , Liang Jane W , Botzheim Bren , Quint Meg , Jeevananthan Athavi , Chi Ellenor , Harmer Madison , Hiatt Laurel , Kowalewski Michaela , Steinberg Benjamin , Tausinga Telisha , Tanner Hannah , Ho Tiffany F , Mark Bayarmaa , Zenger Brian , Hu Sophia , Gebregzabheir Amanuail , Penny Justin M , Loeb Danielle F , Strickland Tyler , Iwamoto Sean J , Rothman Micol S , Hamnvik Ole-Petter R , Ariel Danit 
Published: January 2025

Abstract

Context
Guidelines for use of injectable estradiol esters (valerate [EV] and cypionate [EC]) among transgender and gender-diverse (TGD) individuals designated male at birth vary considerably, with many providers noting supraphysiologic serum estradiol concentrations based on current dosing recommendations.

Objectives
This work aimed to 1. determine the dose of injectable estradiol (subcutaneous [SC] and intramuscular [IM]) needed to reach guideline-recommended estradiol concentrations for TGD adults using EC/EV; 2. describe the relationship between estradiol concentration relative to timing/dose of last estradiol injection and other covariates; and 3. determine dosing differences between IM/SC EV/EC.

Methods
A cross-sectional retrospective study was conducted across 6 US medical centers including TGD adults on same-dose injectable estradiol for more than 75 days, with confirmed timing of estradiol concentration relative to last injection, from January 1, 2019 to December 31, 2023. Descriptive statistics were used to describe patient characteristics and weighted linear mixed models to evaluate relationship between various covariates and estradiol concentration.

Results
Data from 562 patients were included. Among those injecting every 7 days who reached the guideline-recommended estradiol concentration (n = 131, 27.5%), the median estradiol dose was 4.0 mg (interquartile range, 3.0-5.0 mg). Among all patients, the majority reached supraphysiologic estradiol concentrations (&gt;200 pg/mL [&gt;734 pmol/L]) while dose and timing in the injection cycle were significant covariates for the estradiol concentration. There were no significant dosing differences between IM/SC EV/EC.

Conclusion
Injectable estradiol esters effectively reach guideline-recommended estradiol concentrations but at lower doses than previously recommended. Estradiol concentrations are best interpreted relative to timing of last injection. Route of administration and type of ester do not significantly affect estradiol concentrations.
Veteran and healthcare team perspectives on the TeleWound Practice Program within the Veterans Health Administration
Authors: Wirth Marissa , Etingen Bella , Hogan Timothy P , Smith Bridget M , Tarlov Elizabeth , Stroupe Kevin , Kartje Rebecca , Weaver Frances M 
Published: January 2025

Objective: The Veterans Health Administration (VHA) recently piloted the implementation of the TeleWound Practice Program (TWP), which provides interprofessional wound care to Veterans remotely. We assessed the perceptions of Veterans and healthcare team members (HCTMs), and their experiences with the TWP. Method: We surveyed Veterans from four VHA medical centres who had received at least one TWP visit between 1 May 2020 and 31 May 2021, and HCTMs associated with any TWP encounter between 1 September 2019 and 31 March 2021. Survey data were summarised using descriptive statistics and open-ended question responses were analysed using thematic coding. Results: Out of 534 Veterans approached, 194 completed the survey (a 36% response rate). Most were interested in continuing to use TeleWound care (66%), felt more motivated to participate in their wound care due to the TWP (70%), and reported reductions in travel distance (81%) and cost (81%) related to wound care. Of the 32 HCTMs approached, 19 completed the TWP survey (a 59% response rate). Respondents indicated that the TWP improved their own professional decision-making skills (82%) and supported Veterans to take a more active role in their health (100%). Challenges included insufficient training for HTCMs, lack of stakeholder buy-in, and logistical and technical issues. Suggestions for improvement related to equipment, additional training and dedicated TWP staff. Conclusion: In this study, the Veterans were satisfied with the TWP and were more motivated to engage in wound self-management after receiving care through the TWP. HCTMs also perceived the TWP as beneficial to Veterans. However, additional efforts are needed to address barriers to TWP implementation across the VHA system of care.
The provision of wound care and management in a community healthcare setting: an exploratory study
Authors: Skerritt Louise , Gooney Martin , Sheahan Linda 
Published: January 2025

Objective: Wound management can be costly and challenging to the health services' scarce resources. Information regarding the number of wounds in a community care setting and their associated aetiology will provide nurses and nurse managers with an insight into the specific needs of these clients with wounds and highlight areas where care or services can be improved or further developed. This research aimed to establish the prevalence and aetiology of wounds, the current delivery of wound care, wound documentation and referral pathways in an Irish community care setting. Method: A retrospective chart review was carried out on all clients in the community care area who received wound care from the Public Health Nursing Service over a specified one-year period. Results: A total of 331 individuals were identified as having wounds, and nearly half presented with more than one wound, equating to 632 wounds in total (point prevalence (PP)=0.46%). A total of 56% (n=186; PP=0.14%) had leg ulcers (LUs), 18% (n=58; PP=0.04%) had pressure ulcers (PUs), 15% (n=49; PP=0.04%) had developed a diabetic foot ulcer (DFUs) and a further 11% (n=38; PP=0.03%) had wounds of other aetiologies. The mean duration of wounds was 11.37 months. Comorbidities were present in 99% (n=327) of clients with wounds, with cardiovascular disease observed in 87% (n=288) of clients and diabetes in 45% (n=148). Nursing wound-related concerns resulted in 52% (n=171) of clients receiving antibiotics, with 71% (n=121) being prescribed more than one dose. As many as 61% (n=104) of clients prescribed antibiotics did not have completed documentation to demonstrate a suspected wound infection. It was established that 16% of the Public Health Nursing Service's active caseload was made up of clients with wounds and the management of these wounds accounted for 67% of nursing time, equating to 28 full-time community nurses. Conclusion: This study has identified that people with chronic (hard-to-heal) wounds often present with more than one chronic disease, which may negatively influence the wound's healing trajectory, lengthening its duration. The criteria for onward referral for suspected wound infections have been examined and resulted in large numbers of poorly documented wound assessments, leading to a high reliance on the use of oral antibiotics as commonplace for the management of hard-to-heal wounds. Hard-to-heal wounds, such as lower LUs, PUs and DFUs, are either caused or significantly affected by the presence of underlying comorbidities. Therefore, aligning the prevention and management of these burdensome wounds with National Clinical Programmes will deliver efficient, cost-effective, holistic quality care to clients in Irish community healthcare settings.
Bronchopleural fistula following application of Hem-o-lock clip at bronchial stump after lobectomy: A case report
Authors: Li Qian-Yu , Wang Xiao-Long , Zhang Feng , Wei Hai-Tao 
Published: January 2025

BACKGROUND
Hem-o-lock clip, a versatile and reliable non-absorbable tissue clip, has gained widespread acceptance in laparoscopic surgeries for vessel ligation and tissue approximation. Its efficacy and safety have been well-documented.
CASE SUMMARY
This case report describes the occurrence of a bronchopleural fistula following the application of the Hem-o-lock clip for the treatment of a lobar bronchial stump after lobectomy.
CONCLUSION
This case underscores the importance of exercising caution when using the Hem-o-lock clip for the management of non-vascular tissues during thoracic surgery.
Difficulty removing a totally implantable venous access port: A case report
Authors: Chen Jing , Tang Mu , Han Qin-Yuan , Tang Lei , Yu Teng-Hua , Zhao Yan-Ping , He Chong-Wu 
Published: January 2025

BACKGROUND
This case report examines the challenges associated with removing a totally implantable venous access port (TIVAP) used for long-term chemotherapy in a patient with breast cancer. Prolonged use of TIVAPs can result in complications such as catheter kinking, thrombosis, and adhesions between the catheter and surrounding tissues, potentially complicating their removal.
CASE SUMMARY
A breast cancer patient with bone metastasis presented with difficulty aspirating blood from a TIVAP that had been placed in the right internal jugular vein for 3 years. Initial removal attempts at the Department of Venous Access Center were unsuccessful, likely due to adhesions, necessitating a subsequent successful catheter extraction in a hybrid operating room. Imaging revealed no abnormalities, and the catheter was removed using a mosquito clamp to detach it from surrounding tissues.
CONCLUSION
This case highlights the challenges of removing TIVAPs inserted via the internal jugular vein, particularly when the catheter traverses the sternocleidomastoid muscle. Repeated neck movements might lead to significant adhesions around the catheter, complicating its removal. Careful consideration should be given during catheter placement to avoid muscle-related adhesions and facilitate smoother extraction in long-term use.
Percheron syndrome with memory impairment as chief manifestation: A case report
Authors: Nong Xi-Feng , Cao Xiao , Tan Xing-Lin , Jing Li-Yan , Liu Hao 
Published: January 2025

BACKGROUND
Percheron acute artery occlusion is a rare type of acute cerebral infarction.
CASE SUMMARY
An elderly male presented with sudden-onset near-memory and sensory impairments for 5 days. Upon admission, based on symptoms, signs, magnetic resonance imaging, and computed tomography findings, a diagnosis of Percheron syndrome was made. Subsequently, anti-platelet therapy, lipid-lowering treatment, cerebral circulation enhancement (such as the administration of calcium channel blockers to improve cerebral blood flow), and neurotrophic support (such as the use of drugs like citicoline to protect nerve cells) were immediately implemented, along with additional symptomatic treatments. The patient’s symptoms were alleviated, following which he was discharged.
CONCLUSION
The diagnosis of acute occlusion of the Percheron artery requires rich clinical expertise and accurate imaging tools. Timely intervention and effective follow-up hold significant implications for optimizing patient recovery.