bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-sensitization between legumes is frequently seen, but variable and not always clinically relevant
Authors: Smits Mark , Verhoeckx Kitty , Knulst André , Welsing Paco , de Jong Aard , Gaspari Marco , Ehlers Anna , Verhoeff Paulien , Houben Geert , Le Thuy-My 
Published: March 2023

BackgroundFood allergy to peanut and soybean, both legumes, is highly prevalent. The consumption of other legumes and legume protein isolates, some of which may be considered novel foods, is increasing. This may lead to an increase in sensitization and allergy and may pose a risk for legume-allergic (e.g. peanut and soybean) patients due to cross-reactivity.ObjectiveThis study investigated the frequency of co-sensitization and co-allergy between legumes and the role of different protein families.MethodsSix legume-allergic patient groups were included: peanut (n = 30), soybean (n = 30), lupine (n = 30), green pea (n = 30), lentil (n = 17), bean (n = 9). IgE binding to total extracts, protein fractions (7S/11S globulin, 2S albumin, albumin), and 16 individual proteins from 10 legumes (black lentil, blue lupine, chickpea, faba bean, green lentil, pea, peanut, soybean, white bean, and white lupine) was measured by line blotResultsCo-sensitization varied from 36.7% to 100%. Mono-sensitization was only found in soybean (16.7%), peanut (10%), and green pea-allergic (3.3%) patients. A high frequency of co-sensitization between the 7S/11S globulin fractions of all 10 legumes and individual 7S and 11S globulins was observed. In peanut and soybean-allergic patients, co-allergies for other legumes were uncommon (≤16,7%), while in green pea, lupine, lentil, and bean-allergic patients co-allergy for peanut (64.7%–77.8%) or soybean (50%–64.7%) was frequently seen.ConclusionCo-sensitization between legumes was high, but generally not clinically relevant. Co-allergy to other legumes was not often seen in peanut- and soybean allergic patients. The 7S and 11S globulins were likely responsible for the observed co-sensitization.
Mechanical disturbances applied by motorized ankle foot orthosis to adapt ankle muscles activation—A validation study
Authors: Asín-Prieto Guillermo , Oliveira Barroso Filipe , Martínez-Expósito Aitor , Urendes Eloy , Gonzalez-Vargas Jose , Moreno Juan C. 
Published: March 2023

Background: Reduced function of ankle muscles usually leads to impaired gait. Motorized ankle foot orthoses (MAFOs) have shown potential to improve neuromuscular control and increase volitional engagement of ankle muscles. In this study, we hypothesize that specific disturbances (adaptive resistance-based perturbations to the planned trajectory) applied by a MAFO can be used to adapt the activity of ankle muscles. The first goal of this exploratory study was to test and validate two different ankle disturbances based on plantarflexion and dorsiflexion resistance while training in standing still position. The second goal was to assess neuromuscular adaptation to these approaches, namely, in terms of individual muscle activation and co-activation of antagonists.Methods: Two ankle disturbances were tested in ten healthy subjects. For each subject, the dominant ankle followed a target trajectory while the contralateral leg was standing still: a) dorsiflexion torque during the first part of the trajectory (Stance Correlate disturbance—StC), and b) plantarflexion torque during the second part of the trajectory (Swing Correlate disturbance—SwC). Electromyography was recorded from the tibialis anterior (TAnt) and gastrocnemius medialis (GMed) during MAFO and treadmill (baseline) trials.Results: GMed (plantarflexor muscle) activation decreased in all subjects during the application of StC, indicating that dorsiflexion torque did not enhance GMed activity. On the other hand, TAnt (dorsiflexor muscle) activation increased when SwC was applied, indicating that plantarflexion torque succeeded in enhancing TAnt activation. For each disturbance paradigm, there was no antagonist muscle co-activation accompanying agonist muscle activity changes.Conclusion: We successfully tested novel ankle disturbance approaches that can be explored as potential resistance strategies in MAFO training. Results from SwC training warrant further investigation to promote specific motor recovery and learning of dorsiflexion in neural-impaired patients. This training can potentially be beneficial during intermediate phases of rehabilitation prior to overground exoskeleton-assisted walking. Decreased activation of GMed during StC might be attributed to the unloaded body weight in the ipsilateral side, which typically decreases activation of anti-gravity muscles. Neural adaptation to StC needs to be studied thoroughly in different postures in futures studies.
Identification of circular RNAs expression pattern in caprine fetal fibroblast cells exposed to a chronic non-cytotoxic dose of graphene oxide-silver nanoparticle nanocomposites
Authors: Yuan Yu-Guo , Xing Yi-Tian , Liu Song-Zi , Li Ling , Reza Abu Musa Md Talimur , Cai He-Qing , Wang Jia-Lin , Wu Pengfei , Zhong Ping , Kong Il-Keun 
Published: March 2023

The widespread use of graphene oxide-silver nanoparticle nanocomposites (GO-AgNPs) in biomedical sciences is increasing the chances of human and animal exposure to its chronic non-toxic doses. Exposure to AgNPs-related nanomaterials may result in the negative effect on the dam, fetus and offspring. However, there are only little available information for profound understanding of the epigenetic alteration in the cells and animals caused by low-dose chronic exposure of GO-AgNPs. The present study investigated the effect of 0.5 μg/mL GO-AgNPs for 10 weeks on the differential expression of circular RNAs (circRNAs) in caprine fetal fibroblast cells (CFFCs), and this dose of GO-AgNPs did not affect cell viability and ROS level. We predicted the functions of those differentially expressed (DE) circRNAs in CFFCs by bioinformatics analysis. Furthermore, we validated the expression of ten DE circRNAs using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) to ensure the reliability of the sequencing data. Our results showed that the DE circRNAs may potentially regulate the GO-AgNPs-inducing epigenetic toxicity through a regulatory network consisted of circRNAs, miRNAs and messenger RNAs (mRNAs). Therefore, the epigenetics toxicity is essential to assess the biosafety level of GO-AgNPs.
Single-cell transcriptome profiling reveals enriched memory T-cell subpopulations in hypertension
Authors: Wang Xiaoqi , Wu Xiaobin , Zhang Pei , Zhou Yuan , Cai Jun , Jin Ling 
Published: March 2023

Introduction: The adaptive immune response mediated by T cells plays a vital role in the initiation and maintenance of blood pressure (BP) elevation. Memory T cells, which are antigen-specific T cells, can respond specifically to repeated hypertensive stimuli. Although the roles of memory T cells in animal models are well studied, their maintenance and functions in hypertensive patients are poorly understood.Method: Here, we focused on the circulating memory T cells of hypertensive patients. By using single-cell RNA sequencing technology, subsets of memory T cells were identified. Differentially expressed genes (DEGs) and functional pathways were explored for related biological functions in each population of memory T cells.Result and Discussion: Our study identified four subsets of memory T cells in the blood of hypertensive patients, with CD8 effector memory T (TEM) cells accounting for more cells and demonstrating more biological functions than CD4 TEM cells. CD8 TEM cells were further analyzed using single-cell RNA sequencing technology, and subpopulation 1 was demonstrated to contribute to BP elevation. The key marker genes CKS2, PLIN2, and CNBP were identified and validated by mass-spectrum flow cytometry. Our data suggest that CD8 TEM cells as well as the marker genes could be preventive targets for patients with hypertensive cardiovascular disease.
Synthesis of strong magnetic response ZIF-67 for rapid adsorption of Cu2+
Authors: Lei Yuanhang , Yang Haibo , Xie Jiangqin , Chen Qi , Quan Wenxuan , Wang Anping 
Published: March 2023

With the acceleration of industrialization and urbanization, global water resources have been polluted. Among the water pollutants, heavy metals have caused great harm to the environment and organisms. When the concentration of Cu2+ in water exceeds the standard, the intake of the human body will mainly damage the nervous system. We use MOF materials with high chemical stability, specific surface area, adsorption, and other unique properties to adsorb Cu2+. MOF-67 was prepared with various solvents, and a stronger magnetic response MOF-67 with the largest surface area and best crystal form were selected. It quickly adsorbs low-concentration Cu2+ in water to purify water quality. At the same time, it can be recovered promptly through an external magnetic field to avoid secondary pollution, which conforms to the concept of green environmental protection. When the initial concentration of Cu2+ is 50 mg/L for 30 min, the adsorption rate reaches 93.4%. The magnetic adsorbent can be reused three times.
Online corporate communication: Should national culture matter?
Authors: Capece Guendalina , Di Pillo Francesca 
Published: March 2023

IntroductionThe purpose of this paper is to investigate how the culture of a nation influences online corporate communication, focusing on the assessment of the culturability of business websites. Although the Internet constitutes a global phenomenon, cultural filters influence its use at the local level, ultimately determining a more or less favorable attitude toward a given website. Understanding and analysing the cultural adaptation of online communication is crucial as it has the potential to greatly influence how customers perceive and respond to the graphic and content elements.MethodsBuilding upon Hofestede's and Hall's theories, a content analysis technique was used to code the cultural markers and new indicators have been created to allow the association of the graphic elements and the contents of the websites with the cultural characteristics. The countries chosen as case studies are India and Australia, which present significant cultural differences and allow highlighting of the practical implications deriving from the cultural adaptation of the website design.ResultsThe results of this paper show that the company websites analyzed are designed to incorporate the cultural nuances of the target country. In particular, on the Australian websites, there is a greater frequency of cultural markers referring to individualism, masculinity, and uncertainty avoidance compared to Indian ones. On the contrary, Indian websites show more cultural markers referring to power distance, high context, and polychronic characteristics than Australian ones. This paper overcomes the qualitative approaches of the previous literature, developing new indicators for website analysis and providing a quantitative comparison with Hofstede and Hall frameworks.DiscussionThis work provides a guideline for companies, analysts, and the different professionals involved in online communication and web design. More specifically, they have to be aware of the cultural challenges when they operate outside the national boundaries, by designing a culturally usable website in one of the countries chosen for this study.
Improved computation of Lagrangian tissue displacement and strain for cine DENSE MRI using a regularized spatiotemporal least squares method
Authors: Ghadimi Sona , Abdi Mohamad , Epstein Frederick H. 
Published: March 2023

IntroductionIn displacement encoding with stimulated echoes (DENSE), tissue displacement is encoded in the signal phase such that the phase of each pixel in space and time provides an independent measurement of absolute tissue displacement. Previously for DENSE, estimation of Lagrangian displacement used two steps: first a spatial interpolation and, second, least squares fitting through time to a Fourier or polynomial model. However, there is no strong rationale for such a through-time model,MethodsTo compute the Lagrangian displacement field from DENSE phase data, a minimization problem is introduced to enforce fidelity with the acquired Eulerian displacement data while simultaneously providing model-independent regularization in space and time, enforcing only spatiotemporal smoothness. A regularized spatiotemporal least squares (RSTLS) method is used to solve the minimization problem, and RSTLS was tested using two-dimensional DENSE data from 71 healthy volunteers.ResultsThe mean absolute percent error (MAPE) between the Lagrangian displacements and the corresponding Eulerian displacements was significantly lower for the RSTLS method vs. the two-step method for both x- and y-directions (0.73±0.59 vs 0.83 ±0.1, p &lt; 0.05) and (0.75±0.66 vs 0.82 ±0.1, p &lt; 0.05), respectively. Also, peak early diastolic strain rate (PEDSR) was higher (1.81±0.58 (s-1) vs. 1.56±0. 63 (s-1), p&lt;0.05) and the strain rate during diastasis was lower (0.14±0.18 (s-1) vs 0.35±0.2 (s-1), p &lt; 0.05) for the RSTLS vs. the two-step method, with the former suggesting that the two-step method was over-regularized.DiscussionThe proposed RSTLS method provides more realistic measurements of Lagrangian displacement and strain from DENSE images without imposing arbitrary motion models.
Evaluation of the safety and efficacy of a Polyzene-F nanocoated coronary stent system: A systematic review and single-arm meta-analysis
Authors: Bian Jifang , Yang Rongyuan , Wang Dawei , Yu Huimin , Liu Yuan , Liu Qing 
Published: March 2023

BackgroundA stent for patients with coronary heart disease (CHD) provides a requirement for a long-term antiplatelet therapy because of the high possibility of the development of stent thrombosis. It was against this background that both Cobra and Catania Polyzene-F (PzF) stents were designed to reduce the occurrence of stent thrombosis (ST). In this study, we review the safety and effectiveness of a PzF-nanocoated stent.MethodsThis systematic review with the title was registered in PROSPERO (No.398781). The inclusion criteria were including studies among patients with PzF-nanocoated coronary stents and reported target vessel failure (TVF) and ST as the outcomes, and the exclusion criteria were excluding reported patients who could not receive the adjunctive medical therapies or without the necessary endpoints. Reports about PzF-nanocoated stents were searched in PubMed, Embase, and Web of Science and other sources. Because of the existence of few reports and a lack of comparison groups, a single-arm meta-analysis was conducted in R software (v3.6.2), using a random-effects model with the generic inverse variance method. After a heterogeneity test, assessment of evidence quality was conducted by using GRADE software. A funnel plot Egger's test was performed to evaluate publication bias, and a sensitivity analysis was done to determine the robustness of the overall effects.ResultsSix studies of 1,768 subjects were included. The primary endpoint that pooled the TVF rate was 8.9% (95% CI 7.5%–10.2%), which comprised the pooled cardiac death (CD) rate (1.5%, 95% CI 0%–3%), myocardial infarction (MI) rate (2.7%, 95% CI 0.4%–5.1%), target vessel revascularization (TVR) (4.8%, 95% CI 2.4%–7.2%), or target lesion revascularization (TLR) (5.2%, 95% CI 4.2%–6.4%), while the secondary endpoint ST was 0.4% (95% CI 0.1%–0.9%). The funnel plots of TVF, CD, TVR, and TLR did not show any serious publication bias, and TVF, TVR, and TLR showed evidence of moderate quality in GRADE assessment. The sensitivity analysis showed that TVF, TLR, and ST exhibited good stability (I2 = 26.9%, 16.4%, and 35.5%, respectively), while the other endpoints showed moderate instability.ConclusionThese data indicated that the PzF-nanocoated coronary stents of the Cobra and Catania systems demonstrated good safety and efficacy in clinical application. However, the sample size of patients included in the reports was relatively small, and this meta-analysis will be updated if more studies are published in the future.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42023398781
Detection of electromagnetic anomalies over seismic regions during two strong (MW > 5) earthquakes
Authors: Recchiuti D. , D’Angelo G. , Papini E. , Diego P. , Cicone A. , Parmentier A. , Ubertini P. , Battiston R. , Piersanti M. 
Published: March 2023

Ionospheric disturbances (such as electromagnetic emissions) in connection to strong earthquakes have been reported in literature for over two decades. In order to be reliable, the identification of such disturbances requires a preliminary robust definition of the ionospheric background in the absence of both seismic activity and any other possible input (e.g., transient change in solar activity).In this work, we present a new technique for the assessment of the electromagnetic (EM) background in the ionosphere over seismic regions. The background is estimated via a multiscale statistical analysis that makes use of most of the electric- and magnetic-field datasets (2019–2021) from the China Seismo-Electromagnetic Satellite (CSES-01).The result is a map of the average relative energy in a 6° x 6° LAT-LON cell centered at the earthquake epicenter (EE). Only EM signals that statistically differ from the background should be considered as events suitable for investigation.The method is tested against two strong seismic events, the 14 August 2021 Haitian earthquake (7.2 MW) and the 27 September 2021 Cretan earthquake (6.0 MW). In the former case, a signal (with characteristic frequency of 250 Hz) can be identified, which emerges from the background. In the latter one, the concurrent strong geomagnetic activity does not allow to tell any distinct signal apart from the background.
Lifelong learning processes in professional development for online teachers during the Covid era
Authors: Reychav Iris , Elyakim Nitzan , McHaney Roger 
Published: March 2023

IntroductionLifelong learning encompasses four pillars: (1) learning to know; (2) learning to do; (3) learning to be; and (4) learning to live together. These four pillars, which are expanded upon within the current study provide a lens to examine relationships between professional development, use of technology for remote online teaching, and pedagogical efficacy in the age of COVID-19.MethodsThe study examined survey responses from 372 primary school subjects representing six different districts, and interviews with 16 teachers. The responses were analyzed using correlation and MANOVA statistical tests.ResultsTeachers’ professional development processes were found to have a positive impact on the teachers’ pedagogical efficacy, by means of positive perceptions toward the use of technology for remote online teaching. Learning foci predicted the use of technology, and the use of technology predicted learning assessment measurements.DiscussionThe study’s findings reinforce the need for professional development processes based on systematic identification of pedagogical needs that arise in the field, their analysis and the understanding of the added value of pedagogical tools that can support enhanced implementation of teaching - learning - evaluation. The study’s findings point to epistemological elements related to the types of acquired knowledge and to learning methods, which make it possible to differentiate between various processes in teachers’ professional development as well as processes in the use of innovative pedagogical technology.
SDHB immunohistochemistry for prognosis of pheochromocytoma and paraganglioma: A retrospective and prospective analysis
Authors: Su Tingwei , Yang Yifan , Jiang Lei , Xie Jing , Zhong Xu , Wu Luming , Jiang Yiran , Zhang Cui , Zhou Weiwei , Ye Lei , Ning Guang , Wang Weiqing 
Published: March 2023

IntroductionPheochromocytomas and paragangliomas (PCC/PGL) are rare neuroendocrine tumors and can secrete catecholamine. Previous studies have found that SDHB immunohistochemistry (IHC) can predict SDHB germline gene mutation, and SDHB mutation is closely associated with tumor progression and metastasis. This study aimed to clarify the potential effect of SDHB IHC as a predictive marker for tumor progression in PCC/PGL patients.MethodsWe included PCC/PGL patients diagnosed in Ruijin Hospital, Shanghai Jiao Tong University School of Medicine from 2002 to 2014 for retrospective analysis and discovered that SDHB (-) staining patients had poorer prognoses. Then we examined SDHB protein expression by IHC on all tumors in the prospective series, which was composed of patients from 2015 to 2020 in our center.ResultsIn the retrospective series, the median follow-up was 167 months, and during follow-up, 14.4% (38/264) patients developed metastasis or recurrence, and 8.0% (22/274) patients died. Retrospective analysis revealed that 66.7% (6/9) of participants in the SDHB (-) group and 15.7% (40/255) of those in the SDHB (+) group developed progressive tumors (OR: 10.75, 95% CI: 2.72-52.60, P=0.001), and SDHB (-) was independently associated with poor outcomes after adjusting by other clinicopathological parameters (OR: 11.68, 95% CI: 2.58-64.45, P=0.002). SDHB (-) patients had shorter disease-free survival (DFS) and overall survival (OS) (P&lt;0.001) and SDHB (-) was significantly associated with shorter median DFS (HR: 6.89, 95% CI: 2.41-19.70, P&lt;0.001) in multivariate cox proportional hazard analysis. In the prospective series, the median follow-up was 28 months, 4.7% (10/213) patients developed metastasis or recurrence, and 0.5% (1/217) patient died. For the prospective analysis, 18.8% (3/16) of participants in the SDHB (-) group had progressive tumors compared with 3.6% (7/197) in the SDHB (+) group (RR: 5.28, 95% CI: 1.51-18.47, P=0.009), statistical significance remained (RR: 3.35, 95% CI: 1.20-9.38, P=0.021) after adjusting for other clinicopathological factors.ConclusionsOur findings demonstrated patients with SDHB (-) tumors had a higher possibility of poor outcomes, and SDHB IHC can be regarded as an independent biomarker of prognosis in PCC/PGL.
Identification of five hub immune genes and characterization of two immune subtypes of osteoarthritis
Authors: Pan Lifeng , Yang Feng , Cao Xianhua , Zhao Hongchang , Li Jian , Zhang Jinxi , Guo Jiandong , Jin Zhijiang , Guan Zhongning , Zhou Feng 
Published: March 2023

BackgroundOsteoarthritis (OA) is one of the most prevalent chronic diseases, leading to degeneration of joints, chronic pain, and disability in the elderly. Little is known about the role of immune-related genes (IRGs) and immune cells in OA.MethodHub IRGs of OA were identified by differential expression analysis and filtered by three machine learning strategies, including random forest (RF), least absolute shrinkage and selection operator (LASSO), and support vector machine (SVM). A diagnostic nomogram model was then constructed by using these hub IRGs, with receiver operating characteristic (ROC) curve, decision curve analysis (DCA), and clinical impact curve analysis (CICA) estimating its performance and clinical impact. Hierarchical clustering analysis was then conducted by setting the hub IRGs as input information. Differences in immune cell infiltration and activities of immune pathways were revealed between different immune subtypes.ResultFive hub IRGs of OA were identified, including TNFSF11, SCD1, PGF, EDNRB, and IL1R1. Of them, TNFSF11 and SCD1 contributed the most to the diagnostic nomogram model with area under the curve (AUC) values of 0.904 and 0.864, respectively. Two immune subtypes were characterized. The immune over-activated subtype showed excessively activated cellular immunity with a higher proportion of activated B cells and activated CD8 T cells. The two phenotypes were also seen in two validation cohorts.ConclusionThe present study comprehensively investigated the role of immune genes and immune cells in OA. Five hub IRGs and two immune subtypes were identified. These findings will provide novel insights into the diagnosis and treatment of OA.