bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automated counting ofDrosophilaimaginal disc cell nuclei
Authors: Bosch Pablo Sanchez , Axelrod Jeffrey D. 
Published: May 2023

AbstractAutomated image quantification workflows have dramatically improved over the past decade, enriching image analysis and enhancing the ability to achieve statistical power. These analyses have proved especially useful for studies in organisms such asDrosophila melanogaster, where it is relatively simple to obtain high sample numbers for downstream analyses. However, the developing wing, an intensively utilized structure in developmental biology, has eluded efficient cell counting workflows due to its highly dense cellular population. Here, we present efficient automated cell counting workflows capable of quantifying cells in the developing wing. Our workflows can count the total number of cells or count cells in clones labeled with a fluorescent nuclear marker in imaginal discs. Moreover, by training a machine-learning algorithm we have developed a workflow capable of segmenting and counting twin-spot labeled nuclei, a challenging problem requiring distinguishing heterozygous and homozygous cells in a background of regionally varying intensity. Our workflows could potentially be applied to any tissue with high cellular density, as they are structure-agnostic, and only require a nuclear label to segment and count cells.
Genetic and environmental manipulation ofArabidopsishybridization barriers uncover antagonistic functions in endosperm cellularization
Authors: Bjerkan Katrine N. , Alling Renate M. , Myking Ida V. , Brysting Anne K. , Grini Paul E. 
Published: May 2023

AbstractSpeciation by reproductive isolation can occur by hybridization barriers acting in the endosperm of the developing seed. The nuclear endosperm is a nutrient sink, accumulating sugars from surrounding tissues, and undergoes coordinated cellularization, switching to serve as a nutrient source for the developing embryo. Tight regulation of cellularization is therefore vital for seed and embryonic development. Here we show that hybrid seeds from crosses betweenArabidopsis thalianaas maternal contributor andA. arenosaorA. lyrataas pollen donors result in an endosperm based post-zygotic hybridization barrier that gives rise to a reduced seed germination rate. Hybrid seeds display opposite endosperm cellularization phenotypes, with late cellularization in crosses withA. arenosaand early cellularization in crosses withA. lyrata. Stage specific endosperm reporters display temporally ectopic expression in developing hybrid endosperm, in accordance with the early and late cellularization phenotypes, confirming a disturbance of the source-sink endosperm phase change. We demonstrate that the hybrid barrier is under the influence of abiotic factors, and show that a temperature gradient leads to diametrically opposed cellularization phenotype responses in hybrid endosperm withA. arenosaorA. lyrataas pollen donors. Furthermore, differentA. thalianaaccession genotypes also enhance or diminish seed viability in the two hybrid cross-types, emphasizing that both genetic and environmental cues control the hybridization barrier. We have identified anA. thalianaMADS-BOX type I family single locus that is required for diametrically opposed cellularization phenotype responses in hybrid endosperm. Loss of AGAMOUS-LIKE 35 significantly affects the germination rate of hybrid seeds in opposite directions when transmitted through theA. thalianaendosperm, and is suggested to be a locus that promotes cellularization as part of an endosperm based mechanism involved in post-zygotic hybrid barriers. The role of temperature in hybrid speciation and the identification of distinct loci in control of hybrid failure have great potential to aid the introduction of advantageous traits in breeding research and to support models to predict hybrid admixture in a changing global climate.
Serotonergic reinforcement of a complete swallowing circuit
Authors: Schoofs Andreas , Miroschnikow Anton , Schlegel Philipp , Zinke Ingo , Schneider-Mizell Casey M , Cardona Albert , Pankratz Michael J 
Published: May 2023

SUMMARYHow the body interacts with the brain to perform vital life functions such as feeding is one of the fundamental questions in physiology and neuroscience. Here, we use a whole-animal scanning transmission electron microscopy dataset ofDrosophilato map out the neuronal circuits that connect the entire enteric nervous system to the brain via the insect vagus nerve at synaptic resolution. This revealed a periphery-brain feedback loop in which Piezo-expressing mechanosensory neurons sense food intake and convey that information onto serotonergic neurons within the brain. These serotonergic neurons integrate the interoceptive information with external and central inputs, and in turn stabilize rhythmic activity of serotonin receptor 7 expressing peripheral motor neurons that drive swallowing. Strikingly, the very same motor neurons also share an efference copy of their activity with the aforementioned mechanosensory neurons, thereby closing the motor-sensory-modulatory loop. Our analysis elucidates an elemental, albeit surprisingly complex reinforcement circuit in which rhythmic motor patterns are stabilized through afferent signaling to central serotonergic neurons upon completion of a rewarding action. The circuit motif is constructed to allow the distinction between self-generated action and those in response to the environment.
Natural wine as an expression of sustainability: an exploratory analysis of Italy's restaurant industry
Authors: Gazzola Patrizia , Pavione Enrica , Grechi Daniele , Scavarda Federica 
Published: May 2023

PurposeAlthough not yet fully defined, natural wine represents a sector that has gained the widespread attention of final consumers and, therefore, also of the restaurant world, because of its promise of sustainability. The objective of this paper is to understand Italian haute cuisine's interest in natural wine, with the aim of analysing what qualifies this product as sustainable.Design/methodology/approachAfter introducing a theoretical framework based on the concept of natural wine, a brief paragraph is dedicated to consumer preferences; subsequently, the analysis focusses on a questionnaire given to restaurateurs to determine the impact that natural wine has had in the Italian context. The results try to identify the importance that restaurateurs give to the characteristics of natural wine and their propensity for using such wine in their own businesses.FindingsThe analysis, conducted on a sample of medium-high range restaurants, highlights their strong interest in natural wine, as a result of the final consumers' attitude towards wine with characteristics attributable to sustainability. The positive perception by restaurateurs is similar across Italy, both geographically and in terms of the size of the restaurant.Originality/valueThe originality of the work is the focus on the world of restaurants. To date, the literature on natural wine remains embryonic and always refers to the final consumer. This research is the first step in a broader study that will involve a greater number of restaurants, extending beyond Italy to all of Europe, with the aim of understanding the real development potential of natural wine.
Conceptualizing the relationship between corporate social advocacy and political polarization
Authors: Parcha Joshua M. 
Published: May 2023

Purpose Corporations are now taking stands on contemporary and controversial social issues that share no obvious connection to the corporations’ business practices. At the same time, political polarization continues to intensify, which begs the question: Are these corporate stands – referred to as corporate social advocacy (CSA) – and political polarization related to each other, and if so, how? The purpose of this study is to provide a conceptualization of the connections between CSA and political polarization through a series of propositions that can be tested in subsequent research studies. Corporations have influence in society, and the ways in which they communicate on controversial social issues could further intensify or help assuage political polarization. Conversely, political polarization may be causing CSA in the first place, which would put into question the legitimacy and desirability of CSA because of the environment from which CSA is cultivated.Design/methodology/approach This study is designed to be conceptual, and the approach is based on theory building.Findings The study conceptualizes the relationship between CSA and political polarization to be symbiotic because both are bidirectional causes of each other. Engagement in CSA is also argued to be positively associated with perceptions that corporations contain particular political ideologies, i.e. more “liberal-leaning” or “conservative-leaning.” This study also predicts that – dependent on particular conditions – CSA will also lead to an increase in both boycotts and skepticism.Practical implications This study will contribute to scholars’, practitioners’ and consumers’ understanding of the causes and effects of CSA. The way in which political polarization is potentially causing CSA puts into question the legitimacy of corporations engaging in CSA in the first place. If CSA is cultivated in the soil of political polarization, is CSA desirable for corporations? Conversely, the way in which CSA is potentially causing political polarization also puts the legitimacy of CSA into question. If CSA is causing political polarization, is CSA desirable for society?Social implications Corporations are an influential part of society, and thus will influence how society views controversial social issues. If the predictions in this study hold, corporations will play an important role in either intensifying or reducing political polarization, and political polarization will also play an important role in how corporations communicate about CSA issues.Originality/value Research focused on CSA is burgeoning, yet limited studies have examined how CSA and political polarization interact. Although there could be positive aspects of corporate involvement in CSA, this study examines some of the potential negative aspects of corporate involvement in CSA. Future research will also be able to test the propositions proposed in this study.
High-involvement work practices, work engagement and their effects on bank employees' turnover intentions: the moderating role of functional competence
Authors: Islam Md Shamirul , Amin Muslim , Feranita Feranita , Karatepe Osman M. 
Published: May 2023

PurposeThis study aims to propose and examine a research model where work engagement mediates the impacts of high-involvement work practices (HIWPs) on bank employees' turnover intentions. Specifically, the paper assesses: (a) the effects of empowerment, information sharing, rewards and training on work engagement and turnover intention, (b) work engagement as a mediator of the effects of these HIWPs on turnover intention (c) and functional competence as a moderator of the effects of these HIWPs on work engagement.Design/methodology/approachAn online survey was employed to gather data from 343 employees working in commercial banks in Bangladesh. The authors applied partial least squares structural equation modeling to assess the aforesaid linkages.FindingsEmpowerment and information sharing increase bank employees' work engagement, while training and rewards reduce their proclivity to leave. Work engagement partly mediates the relationships of empowerment and information sharing to turnover intention. Functional competence moderates the relationship between three HIWPs (empowerment, information sharing and rewards) on work engagement.Originality/valueThe paper examines the association between HIWPs and turnover intention, which has been subjected to little empirical inquiry among bank employees during a crisis (e.g. Covid-19 pandemic). The paper provides new insights into the underlying mechanism linking HIWPs and turnover intention and highlights the moderating effect of functional competence. Additionally, the study offers new knowledge on the impact of the pandemic on bank employees' HIWPs. Finally, this paper used data gathered from bank employees in Bangladesh, which is an underrepresented Asian country in the extant service research.
Unlocking sustained use of clean cooking technologies in Uganda: the influence of technology-specific attributes
Authors: Katutsi Vincent Patsy , Kaberuka Will , Ngoma Muhammed , Yawe Bruno Lule 
Published: May 2023

Purpose
This study aims to establish whether three technology-specific attributes – convenience, compatibility with cultural needs and social reputation – influence the sustained use of clean cooking technologies in Uganda’s households.

Design/methodology/approach
This study adopted a cross-sectional and correlational research design. A survey was conducted with 125 households. The data were analyzed using SPSS and Smart PLS.

Findings
The results indicate that, in contrast to convenience, social reputation and compatibility with cultural needs significantly influence the sustained use of clean cooking technologies in Uganda’s households.

Research limitations/implications
This study focused on the Greater Kampala Metropolitan Area; hence, several households in other urban centers and rural communities were excluded from the study. In addition, technology-specific attributes only accounted for 34.4% of the variation in the sustained use of clean cooking technologies in Uganda, leaving 65.6 unexplained. Therefore, the authors recommend that future studies look at other technology-specific attributes that may influence the sustained use of clean cooking technologies in Uganda’s households.

Originality/value
This study provides initial evidence of technology-specific attributes and the sustained use of clean cooking technologies based on the expectation–confirmation model.
Corruption's effect on BRICS countries' economic growth: a panel data analysis
Authors: Simo-Kengne Beatrice D. , Bitterhout Siphiwo 
Published: May 2023

PurposeThe theoretical debate of corruption's impact on economic growth remains unsettled, making it an empirical question. This study aims to investigate corruption's effect on BRICS countries' economic growth.Design/methodology/approachA panel dataset on BRICS countries spanning 1996 to 2020 was used. Bias-corrected estimators in small dynamic panels were employed to estimate a growth model as a linear-quadratic function of corruption that accounts for cross-sectional dependence, endogeneity and unobserved heterogeneity due to country and time-specific characteristics.FindingsThe results indicate that corruption is detrimental to economic growth in BRICS countries; the quadratic relationship implies corruption is less prevalent in some countries than others. Thus, governments of BRICS countries are encouraged to embark on anti-corruption policies to boost their economic performance.Originality/valueAn important limitation of corruption studies is the difficulty in measuring real corruption experiences due to the secretive nature of corruption and the fact that corruption is known not to leave a paper trail. For the uncertainty of the index estimates, the analysis used a continuous corruption composite score measuring the standard deviation of the extent to which public power is exercised for public gain. Furthermore, estimation and inference are robust to small dynamic panels with a general form of cross-sectional dependence.
Electrical resistivity tomography technique coupled with numerical modelling: A case study for stability analysis
Authors: Bharti Abhay Kumar , Singh Satyendra K. , Pal Sanjit Kumar , Singh Krishna Kant Kumar , Prakash Amar , Bhattacharjee Rana , Kumar Lalan 
Published: May 2023

AbstractCavity due to underground old mine workings is one of the major threats to the coal mines and the overlying subsurface and surface properties, which need to be protected. The detection of old mine workings and stability assessment of overlying strata are common problems in most of the Indian coalfields. Several coal mines in India are loss‐making, mainly due to different types of mine hazards. Khandra mine is one such mine at Raniganj Coalfield, Eastern Coalfields Ltd., a subsidiary of Coal India Limited. In the present study, 2‐dimensional and 3‐dimensional electrical resistivity tomography were carried out for detailed subsurface characterization. It supports delineating underground workings, including the nature of voids/cavities (air or water‐filled). Excessive distortions were reported in electrical resistivity tomography application, especially at the near‐surface, owing to large resistivity variations. Refinement of the model by half‐unit electrode spacing was attempted here to reduce the distortions with minimum possible absolute errors. 3‐Dimensional resistivity volumetric model was also developed with the help of five electrical resistivity tomography parallel profiles for better apprehension of the subsurface. Analysis provided important inputs for stability analysis using 3‐dimensional numerical modelling. The physico‐mechanical properties of the overlying strata, pre‐excavation in situ stresses, boundary conditions and the mine geometry simulation were incorporated for understanding the stability analysis. Stability analysis was carried out using the finite difference technique. The analysis of 3‐dimensional numerical modelling indicated that two distinct layers comprising (i) laterite/part of the course to medium‐grained sandstone and (ii) developed galleries of R‐IX seam exhibited a very low safety factor below 1.0, indicating potholing/subsidence susceptibility. The other three layers comprising parts of fine‐grained sandstone exhibited a relatively higher safety factor of around 2.0, indicating moderately stable zones, but not on a long‐term basis. Parts of Siduli stream embankments need suitable retaining walls to avoid water inundation for the stability of the area.
Does Public Firms’ Mandatory IFRS Reporting Crowd Out Private Firms’ Capital Investment?
Authors: LIU JIANCHENG (DUNCAN) , SHI WEI , ZENG CHENG , ZHANG GUOCHANG 
Published: May 2023

ABSTRACTWe investigate how the mandatory adoption of International Financial Reporting Standards (IFRS) by publicly listed firms in the European Union affects peer private firms. We find that private firms’ capital investment decreases significantly after the IFRS mandate, relative to public firms. Private firms also display decreased investment when benchmarked against firms relatively insulated from the impact of the IFRS mandate, but the magnitude of the effect is smaller in this case. These results are consistent with the hypothesis that mandatory IFRS reporting (combined with other reforms), while increasing public firms’ financing and investment, crowds out funding for private firms. The effect is more pronounced for larger private firms and in industries where public peers have greater external financing needs. Our evidence suggests that financial reporting regulations cause shifts in resource allocation in an economy.
Occupational exposure to traumatic evidence and posttraumatic stress symptoms in forensic science professionals: Prevalence and patterns
Authors: Schiro Samantha , Elwood Lisa S. , Streed Thomas , Kivisto Aaron J. 
Published: May 2023

AbstractForensic science professionals are routinely exposed to potentially traumatizing evidence. This study aimed to estimate the prevalence of occupational posttraumatic stress disorder (PTSD) among forensic science professionals, identify job‐related correlates of PTSD symptoms, and examine the role of social support in mitigating PTSD symptomology. In response to recruitment through the American Academy of Forensic Sciences, the American Society of Crime Laboratory Directors, and Evidence Technology Magazine, 449 forensic science professionals participated in the current study. Results found that 73.5% (n = 330) of the overall sample experienced at least one work‐related traumatic event consistent with meeting Criterion A for PTSD, and these rates were higher for field‐based respondents (n = 203, 87.9%). The prevalence of past‐month provisional PTSD was 21.6% for the full sample. Disaggregated PTSD rates were 29.0% and 14.5% for field‐based and non‐field‐based respondents, respectively. These rates were 6‐ to 8‐fold higher than the past‐year prevalence of PTSD in the general US population, estimated to be 3.5%, and were found to be at least as high as those observed in prior epidemiological research with non‐treatment seeking members of the US military deployed to war zones in Iraq and Afghanistan. Results further showed that social support was protective against PTSD symptomology. The high rates of occupational exposure to trauma and consequent PTSD symptomology observed in this large sample of forensic science professionals highlight the under‐acknowledged psychological risks of these occupations and the need for enhanced attention to mental health resources for these professionals.
Giant panda (Ailuropoda melanoleuca) neonates use broadband calls to communicate with their mothers
Authors: ZHAO Lin , CHENG Jianbin , ZENG Wen , YANG Bo , ZHANG Guiquan , LI Desheng , ZHANG Hemin , BUESCHING Christina D. , LIU Dingzhen 
Published: May 2023

AbstractInfant call structure should have evolved to elicit maximum maternal attention and investment. Neonates of giant pandas produce three types of vocalizations reported to be vitally important in the context of mother–infant communications. However, how cubs, 0–15 days old, communicate with their mothers to elicit maternal care remains unknown. We analyzed 12 different call parameters of 3475 squawks, 1355 squalls, and 491 croaks from 11 captive giant panda (Ailuropoda melanoleuca) neonates from age 0 to 15 days. In playback experiments, we also tested whether mothers could detect ultrasound. Our results show that neonates use broadband calls with ultrasonic frequencies up to 65 kHz to convey information about their physiological needs and to attract maternal care. In playback experiments, we tested if mothers reacted differently to broadband calls (BBC) than to artificially altered calls that included only frequencies <20 kHz (AUDC) or calls that included only frequencies >20 kHz (USC). Playback confirmed that, although adult females responded significantly less often to USC, BBC than to or AUDC, they could detect USC, BBC and generally made appropriate behavioral responses, indicating a potential benefit for neonates to utilize ultrasonic and broadband frequencies. Our findings provide a new insight into mother–infant communication in giant pandas and will be helpful for reducing the mortality of cubs, younger than 1 month old, in captivity.