bluuebunny/crossref_metadata_2025_split huggingface dataset:
A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences
Authors: Gleichman Amy J. , Kawaguchi Riki , Sofroniew Michael V. , Carmichael S. Thomas 
Published: February 2023

AbstractAstrocytes, one of the most prevalent cell types in the central nervous system (CNS), are critically involved in neural function in both health and disease. Genetically manipulating astrocytes is an essential tool in understanding and affecting their roles. Adeno-associated viruses (AAVs) enable rapid genetic manipulation; however, astrocyte specificity of AAVs can be limited, with high off-target expression in neurons and sparsely in endothelial cells. Here, we report the development of a cassette of four copies of six miRNA targeting sequences (4×6T) which triggers transgene degradation specifically in neurons and endothelial cells. When used in combination with the GfaABC1D promoter, 4×6T increases astrocytic specificity of Cre with a viral reporter from <50% to >99% in multiple serotypes in mice, and confers astrocyte specificity in two inducible forms of Cre; Dre; and reporters. We also present empty vectors to add 4×6T to other cargo, independently and in Cre/Dre-dependent forms. This toolbox of AAVs provides a way to rapidly manipulate astrocytes throughout the CNS, is compatible with different AAV serotypes, and demonstrates the efficacy of using multiplexed miRNA targeting sequences to decrease expression in multiple off-target cell populations simultaneously.
Intergenerational effects of parental educational attainment on parenting and childhood educational outcomes: Evidence from MoBa using within-family Mendelian randomization
Authors: Havdahl Alexandra , Hughes Amanda M , Sanderson Eleanor , Ask Helga , Cheesman Rosa , Reichborn-Kjennerud Ted , Andreassen Ole A. , Corfield Elizabeth C. , Hannigan Laurie , Magnus Per , Njølstad Pål R. , Stoltenberg Camilla , Torvik Fartein Ask , Brandlistuen Ragnhild , Smith George Davey , Ystrom Eivind , Davies Neil M 
Published: February 2023

AbstractThe intergenerational transmission of educational attainment from parents to their children is one of the most important and studied relationships in social science. Longitudinal studies have found strong associations between parents’ and their children’s educational outcomes, which could be due to the effects of parents. Here we provide new evidence about whether parents’ educational attainment affects their parenting behaviours and children’s early educational outcomes using within-family Mendelian randomization and data from 40,879 genotyped parent-child trios from the Norwegian Mother, Father and Child Cohort (MoBa) study. We found evidence suggesting that parents’ educational attainment affects their children’s educational outcomes from age 5 to 14. More studies are needed to provide more samples of parent-child trios and assess the potential consequences of selection bias and grandparental effects.
A confirmed association betweenDNAvariants inCAPN9,OSM, andITGAMcandidate genes and the risk of umbilical hernia in pigs
Authors: Wozniak J. , Loba W. , Iskrzak P. , Pszczola M. , Wojtczak J. , Switonski M. , Nowacka‐Woszuk J. 
Published: February 2023

AbstractUmbilical hernia (UH) is one of the most prevalent defects of swine, affecting their welfare and causing considerable economic loss. The molecular mechanisms behind UH in pigs remain poorly understood. The aim of this study was to verify the association between UH and previously reported DNA variants in theCAPN9,OSM,ITGAM, andNUGGCgenes. A case/control study design was applied in two different crossbred cohorts of commercial fatteners containing 412 and 171 pigs, respectively. SNPs withinCAPN9,OSM, andITGAMwere analyzed using Sanger sequencing, and 10 SNPs inCAPN9, five inOSM, and two inITGAMwere identified.A structural variant in theNUGGCgene was studied by droplet‐digital PCR, and an elevated copy number was detected in only a single individual. Significant differences in allele frequencies for four SNPs inCAPN9were detected. The haplotype analysis showed the effect on the risk of UH for two genes. The CAGGA haplotype withinOSMand AT haplotype inITGAMreduced the relative risk of UH by 52% and 45%, respectively, confirming that variants in those genes are associated with the risk of UH in pigs. Moreover, the interaction between theCAPN9haplotype and the sex of animals had also significant impact on UH risk.
COVID‐19‐associated Brugada pattern electrocardiogram: Systematic review of case reports
Authors: Liu Kevin , Chen Kai , Uzunoglu Ekin C. , Rathore Azeem , Deol Tanya , Wu Ele , Elayi Claude S. , Suryanarayana Prakash G. , Keim Stephen G. , Catanzaro John N. 
Published: February 2023

AbstractAimsTo summarize published case reports of patients diagnosed with coronavirus disease 2019 (COVID‐19) and Brugada pattern electrocardiogram (ECG).MethodsThe Preferred Reporting Items for Systematic Reviews and Meta‐Analyses checklist were followed. A literature search was conducted using PubMed, EMBASE, and Scopus up until September 2021. The incidence, clinical characteristics, and management outcomes of COVID‐19 patients with a Brugada pattern ECG were identified.ResultsA total of 18 cases were collected. The mean age was 47.1 years and 11.1% were women. No patients had prior confirmed diagnosis of Brugada syndrome. The most common presenting clinical symptoms were fever (83.3%), chest pain (38.8%), shortness of breath (38.8%), and syncope (16.6%). All 18 patients presented with type 1 Brugada pattern ECG. Four patients (22.2%) underwent left heart catheterization, and none demonstrated the presence of obstructive coronary disease. The most common reported therapies included antipyretics (55.5%), hydroxychloroquine (27.7%), and antibiotics (16.6%). One patient (5.5%) died during hospitalization. Three patients (16.6%) who presented with syncope received either an implantable cardioverter defibrillator or wearable cardioverter defibrillator at discharge. At follow‐up, 13 patients (72.2%) had resolution of type 1 Brugada pattern ECG.ConclusionCOVID‐19‐associated Brugada pattern ECG seems relatively rare. Most patients had resolution of the ECG pattern once their symptoms have improved. Increased awareness and timely use of antipyretics is warranted in this population.
Offspring school performance at age 12 after induction of labor vs non‐intervention at term: A linked cohort study
Authors: Burger Renée J. , Mol Ben W. , Ganzevoort Wessel , Gordijn Sanne J. , Pajkrt Eva , Van Der Post Joris A. M. , De Groot Christianne J. M. , Ravelli Anita C. J. 
Published: February 2023

AbstractIntroductionThe incidence of induction of labor, for both medical reasons and as an elective procedure, has been rising and a further increase in induction of labor following the ARRIVE trial may be expected. The effects of induction of labor at term on childhood neurodevelopment, however, are not well studied. We aimed to study the influence of elective induction of labor for each week of gestation separately from 37 to 42 weeks on offspring school performance at 12 years of age after uncomplicated pregnancies.Material and methodsWe performed a population‐based study among 226 684 liveborn children from uncomplicated singleton pregnancies, born from 37+0 to 42+0 weeks of gestation in cephalic presentation in 2003–2008 (no hypertensive disorders, diabetes or birthweight ≤p5) in the Netherlands. Children with congenital anomalies, of non‐white mothers and born after planned cesarean section were excluded. Birth records were linked with national data on school achievement. We compared, using a fetus‐at‐risk approach and per week of gestation, school performance score and secondary school level at age 12 in those born after induction of labor to those born after non‐intervention, ie spontaneous onset of labor in the same week plus all those born at later gestations. Education scores were standardized to a mean of 0 and a standard deviation of 1 and adjusted in the regression analyses.ResultsFor each gestational age up to 41 weeks, induction of labor was associated with decreased school performance scores compared with non‐intervention (at 37 weeks −0.05 SD, 95% confidence interval [CI] −0.10 to −0.01 SD; adjusted for confounding factors). After induction of labor, fewer children reached higher secondary school level (at 38 weeks 48% vs 54%; adjusted odds ratio [aOR] 0.88, 95% CI 0.82–0.94).ConclusionsIn women with uncomplicated pregnancies at term, consistently, at every week of gestation from 37 to 41 weeks, induction of labor is associated with lower offspring school performance at age 12 and lower secondary school level compared with non‐intervention, although residual confounding may remain. These long‐term effects of induction of labor should be incorporated in counseling and decision making.
Climate change awareness and mitigation practices in small and medium‐sized enterprises: Evidence from Swiss firms
Authors: Fuchs Anita , Mohan Preeya , Strobl Eric 
Published: February 2023

AbstractThe objective of this paper is to investigate climate change awareness and mitigation effort and their associated motivating and limiting factors to pro‐environmental behavior and firm demographics in small and medium‐sized enterprises (SMEs) in Switzerland. For this purpose, a questionnaire was developed, conducted, and analyzed on motivating and limiting factors along with firm demographics, using descriptive statistics and ordinary least squares (OLS) and ordered probit regression models. The results show that Swiss SMEs are in general aware of climate change and their resulting consequences. SMEs have taken steps to help mitigate greenhouse gas emissions through recycling, reduction of energy and water consumption, and use of recycled packaging. SMEs could however improve their environmental impact through low‐carbon means of transport and use of public transport, sustainable raw material inputs, sustainable energy sources, and setting environmental goals and audits. There was a positive association between climate change awareness and mitigation. Motivating factors to pro‐environmental behavior included financial advantage and responsibility towards the environment, while firm size and human resources were limiting factors. Lastly, different mitigation measures might be influenced by different motivating and limiting factors including financial advantage and pressure from interest groups, as well as firm size.
iGlarLixi (insulin glargine 100 U/ml plus lixisenatide) is effective and well tolerated in people with uncontrolled type 2 diabetes regardless of age: A REALI pooled analysis of prospective real‐world data
Authors: Guja Cristian , Kis János Tibor , Haluzík Martin , Bonnemaire Mireille , Bigot Gregory , Tournay Mathilde , Freemantle Nick , Seufert Jochen 
Published: February 2023

AbstractAimTo evaluate the effectiveness and safety in routine clinical practice of insulin glargine/lixisenatide (iGlarLixi) in people with type 2 diabetes (T2D) according to age.MethodsPatient‐level data were pooled from 1316 adults with T2D inadequately controlled on oral antidiabetic drugs with or without basal insulin who initiated iGlarLixi for 24 weeks. Participants were classified into age subgroups of younger than 65 years (N = 806) and 65 years or older (N = 510).ResultsCompared with participants aged younger than 65 years, those aged 65 years or older had a numerically lower mean body mass index (31.6 vs. 32.6 kg/m2), a longer median diabetes duration (11.0 vs. 8.0 years), were more likely to receive prior basal insulin (48.4% vs. 43.5%) and had a lower mean HbA1c (8.93% [74.10 mmol/mol] vs. 9.22% [77.28 mmol/mol]). Similar and clinically relevant reductions in HbA1c and fasting plasma glucose from baseline to week 24 of iGlarLixi therapy were observed regardless of age. At 24 weeks, least‐squares adjusted mean (95% confidence interval [CI]) change in HbA1c from baseline was –1.55% (–1.65% to –1.44%) in those aged 65 years or older and –1.42% (–1.50% to –1.33%) in those aged younger than 65 years (95% CI: –0.26% to 0.00%; P = .058 between subgroups). Low incidences of gastrointestinal adverse events and hypoglycaemic episodes were reported in both age subgroups. iGlarLixi decreased mean body weight from baseline to week 24 in both subgroups (–1.6 kg in those aged ≥ 65 years and –2.0 kg in those aged < 65 years).ConclusionsiGlarLixi is effective and well tolerated in both younger and older people with uncontrolled T2D.
Chronic kidney disease‐associated pruritus in patients undergoing hemodialysis: Xerosis and topical therapy
Authors: Krismi Arum , Danarti Retno , Setiabudi Ida I. Dewa Putu Pramantara , Wirohadidjojo Yohanes W. 
Published: February 2023

AbstractChronic kidney disease‐associated pruritus (CKD‐aP) is a common and distressing symptom for patients with CKD and a difficult challenge for nephrologists and dermatologists. Recent results showed the multifactorial nature of the pathophysiology, and therapeutic trials were only successful in certain subsets of patients. The clinical manifestations are varied, with xerosis being the most common dermatological manifestation and correlated with the intensity of CKD‐aP. A better understanding of the pathophysiology of xerosis in CKD‐aP and appropriate topical treatment could correct xerosis to reduce the intensity of CKD‐aP and improve the patient's quality of life.
The strategic analysis of service mode selection for a ride‐hailing platform
Authors: Guo Dongliang , Fan Zhi‐Ping , Liu Yang 
Published: February 2023

AbstractThis paper examines the service mode selection strategy of a ride‐hailing platform and analyzes the impact of the strategy on consumers and drivers. The platform has three service modes to select from, including Mode L (only low‐quality services), Mode H (only high‐quality services), and Mode HL (high‐ and low‐quality services). We find that each service mode does not affect the price and the wage of the low‐quality service but affects those of the high‐quality service. Additionally, the platform cannot optimally select Mode L. The platform is better off if the car quality difference between high‐ and low‐quality services (CQD) is small and is worse off otherwise, under Mode HL. Counter‐intuitively, it is conditional for Mode HL to bring the highest surpluses to both consumers and drivers, and the conditions are related to CQD, social status utility of consumers and cost ratio between the drivers providing the two types of services.
Inorganic phosphate content does not affect oviposition preference in the invasive pest Drosophila suzukii
Authors: Olazcuaga Laure , Guilhot Robin , Claret Jean‐Loup , Rode Nicolas O. 
Published: February 2023

AbstractThe broad variation in host use among polyphagous insects is well documented but still poorly understood. In numerous pest insects, the proximate mechanisms responsible for variation in oviposition preference among host plants remain to be elucidated. The invasive crop pest, Drosophila suzukii, attacks a wide range of host fruits. Females prefer ovipositing on particular fruit media (blackberry, cherry, blackcurrant) that are rich in phosphorus. As phosphorus is known to be involved in female reproduction in insect species such as Drosophila, it could drive oviposition preference in D. suzukii. Phosphorus is either present as inorganic or organic phosphate in fruits. As the absolute content in macromolecules associated with phosphate in fruits (i.e. proteins and carbohydrates) do not affect oviposition in D. suzukii, we tested for the effect of inorganic phosphate on oviposition preference. We measured the egg‐laying preferences of D. suzukii in a choice environment containing 12 artificial media with increasing content in inorganic phosphate (monopotassium dihydrogen phosphate). In our assay, D. suzukii females did not prefer ovipositing in media with high inorganic phosphate content compared to media with lower inorganic phosphate content. As a confirmation, we verified the previous result of a higher female preference for media made of phosphorus‐rich fruits (blackberry, cherry, blackcurrant). The higher preference for phosphorus‐rich fruits could be driven by macromolecules containing phosphorus (e.g. phospholipids) or by the presence of one or more molecules that do not contain phosphorus, but that happen to be correlated to fruit phosphorus content. Studying the proximate mechanisms driving host use will ultimately help improve the management of D. suzukii and other crop pests.
Effects of TLR2/4 signalling pathway in western mosquitofish (Gambusia affinis) after Edwardsiella tarda infection
Authors: Wang Chong , Chen Qingshi , Tang Manfei , Wei Tianli , Zou Jixing 
Published: February 2023

AbstractGambusia affinis is regarded as an important animal model. Edwardsiella tarda is one of the most serious pathogens affecting aquaculture. The study explores the effects of TLR2/4 partial signalling pathway in G. affinis of E. tarda infection. The study collected the brain, liver, and intestine after E. tarda LD50 and 0.85% NaCl solution challenge at different times (0 h, 3 h, 9 h, 18 h, 24 h, and 48 h). In these three tissues, the mRNA levels of PI3K, AKT3, IRAK4, TAK1, IKKβ, and IL‐1β were substantially enhanced (p < .05) then returned to normal levels. Additionally, Rac1 and MyD88 in liver had different trend with other genes in brain and intestine, which displayed significantly indifference. The overexpression of IKKβ, and IL‐1β indicated that E. tarda also caused immune reaction in intestine and liver, which would be consistent with delayed edwardsiellosis, which causes intestinal lesions and liver and kidney necrosis. Additionally, MyD88 plays a smaller role than IRAK4 and TAK1 in this signalling pathways. This study could enrich the understanding of the immune mechanism of the TLR2/4 signalling pathway in fish and might help to prescribe preventive measures against E. tarda to prevent infectious diseases in fish.
Total Hip Arthroplasty in the Nonparalytic Limb of Residual Poliomyelitis Patients: A Propensity Score Matched Study
Authors: Li Songlin , Chen Xi , Li Shanni , Weng Xisheng , Lin Jin , Jin Jin , Qian Wenwei 
Published: February 2023

ObjectivePoliomyelitis is a rare neuromuscular disease that can cause hip osteoarthritis on the contralateral side due to an abnormal mechanical weight‐bearing state, making some residual poliomyelitis patients candidates for total hip arthroplasty (THA). The aim of this study was to investigate the clinical outcome of THA in the nonparalytic limbs of these patients compared with those of non‐poliomyelitis patients.MethodsPatients treated between January 2007 and May 2021 were retrospectively identified in a single center arthroplasty database. Eight residual poliomyelitis cases that met the inclusion criteria were matched to non‐poliomyelitis cases in a ratio of 1:2 based on age, sex, body mass index (BMI), age‐adjusted Charlson comorbidity index (aCCI), surgeon, and operation date. The hip function, health‐related quality of life, radiographic outcomes, and complications were analyzed with unpaired Student's t test, Mann–Whitney test, Fisher's exact test or analysis of covariance (ANCOVA). Survivorship analysis was determined using the Kaplan–Meier estimator analysis and Gehan‐Breslow‐Wilcoxon test.ResultsAfter a mean follow‐up of about 5 years, patients with residual poliomyelitis had worse postoperative mobility outcomes(P < 0.05), but there was no difference in total modified Harris hip score (mHHS) or European quality of life‐visual analogue scale (EQ‐VAS) between the two groups (P > 0.05). There was no difference in radiographic outcomes or complications between the two groups, and patients had similar postoperative satisfaction (P > 0.05). No readmission or reoperation occurred in the poliomyelitis group (P > 0.05), but the postoperative limb length discrepancy (LLD) in the residual poliomyelitis group was greater than that in the control group (P < 0.05).ConclusionFunctional outcomes, health‐related quality of life improvement were similarly significantly improved in the nonparalytic limb of residual poliomyelitis patients after THA compared with conventional osteoarthritis patients. However, the residual LLD and weak muscle strength of the affected side will still influence mobility, so residual poliomyelitis patients should be fully informed of this outcome before surgery.
Examining the Role of Physical Activity on Psychological Well-Being and Mental Health Postpartum
Authors: Lesser Iris A. , Turgeon Stéphanie , Nienhuis Carl P. , Bean Corliss 
Published: February 2023

Postpartum physical activity can positively impact mental and physical health. There is a need to better understand how physical activity is related to various psychological constructs to support physical activity in postpartum women. Thus, the purpose of this exploratory, quantitative, study was to examine differences between postpartum women who were physically active and those who were physically inactive on psychological (e.g., self-compassion) and mental health constructs. Five hundred twenty-five women (Mage = 28.4) completed an online survey. Participants who reported being active following the birth of their last child had significantly higher exercise self-efficacy, self-compassion, and basic psychological needs fulfillment for exercise and significantly lower levels of perceived fatigue, anxiety, and depression compared with their inactive counterparts. However, active mothers had lower body satisfaction than inactive mothers. Women who are active after the birth of a child have improved psychological constructs that may benefit overall well-being and mental health during this challenging transition.
Impact on direct and indirect costs of switching patients with inflammatory bowel disease from intravenous to subcutaneous infliximab (CT-P13)
Authors: Carbery Isabel , Burdge Gemma , Clark Tanya , Broglio Giacomo , Greer Dan , Alakkari Alaa , Selinger Christian Philipp 
Published: February 2023

BackgroundWe aim to compare the real-life direct and indirect costs of switching patients from intravenous to subcutaneous (SC) CT-P13, an infliximab biosimilar, in a tertiary UK Inflammatory Bowel Disease (IBD) centre.MethodsAll adult patients with IBD on standard dosing CT-P13 (5 mg/kg 8 weekly) were eligible to switch. Of 169 patients eligible to switch to SC CT-P13, 98 (58%) switched within 3 months and one moved out of area.ResultsTotal annual intravenous cost for 168 patients was £689 507.04 (direct=£653 671.20, indirect=£35 835.84). After the switch, as-treated analysis demonstrated total annual cost for 168 patients (70 intravenous and 98 SC) was £674 922.83 (direct = £654 563, indirect = £20 359.83) resulting in £891.80 higher cost to healthcare providers. Intention to treat analysis showed a total annual cost of £665 961.01 (direct = £655 200, indirect = £10 761.01) resulting in £1528.80 higher cost to healthcare providers. However, in each scenario, the significant decrease in indirect costs resulted in lower total costs after switching to SC CT-P13.ConclusionsOur real-world analysis demonstrates switching from intravenous to SC CT-P13 is broadly cost neutral to healthcare providers. SC preparations have marginally higher direct costs, switching allows for efficient use of intravenous infusion units and reduces costs to patients.