bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interindividual variation of the organism - as an indicator for assessing the severity of heat stroke in an experimental study of Wistar rats
Authors: Dervisevic Emina , Dervisevic Lejla , Ajanović Zurifa , Salihbegović Adis , Mujarić Ekrema , Katica Muhamed 
Published: December 2022

Abstract
Introduction: Sudden deaths during efforts that are multifactorial and associated with exposure of the body to high temperatures beyond the power of thermoregulatory mechanisms are increasingly common. Autopsies are often performed, but the evidence is insufficient and non-specific. The research aimed to determine the core temperature values of rats exposed to different water temperatures (37°C, 41°C, 44°C), before the start of the experiment (Tb), after immersion in water (Tu), after 20 minutes of exposure (Tu) and at death. (Ts) rats for hyperthermia and heat stroke.
Material and Method: Forty rats were divided into five groups depending on the temperature and length of exposure to water: control group-CG37, G41-hyperthermia- group which exposure whosewas a 20 minutes at 41°C, G41-heat stroke- group exposed until death at 41°C, G44- hyperthermia- group which exposure time was 20 minutes on 44°C, G44- heat stroke- group exposed until death on 44°C. A RET-4 probe was used to measure the core temperature of rats.
Results: Significant changes in the body temperature of rats were observed during the lethal outcome, p<0.0005. After exposure to water temperature for a period of 20 minutes, depending on the group, it was observed that the body temperatures of rats differed significantly between G37 and G41, KG37 and G44, p<0.0005 and G41 and G44, p<0.0005. A significant difference was also observed in the postmortem temperature of groups G41 and G44, p=0.01. a significant difference between body temperatures in groups CG37, G41-hyperthermia, G41- heat stroke, G44-hyperthermia and G44-heat stroke (p<0.0005), and the significance of the differences in the CG37 group was p=0.044.
Conclusion: Exposure of albino rats to different water temperatures also led to a change in the internal temperature; normothermia was established through thermoregulation in the control group, and in the other groups, hyperthermia and heat stress occurred.
The effect of problem-solving and assertiveness training on self-esteem and mental health of female adolescents: a randomized clinical trial
Authors: Golshiri Parastoo , Mostofi Alireza , Rouzbahani Shiva 
Published: December 2022

Abstract
Background

Children and adolescents are the assets of the society and their mental and physical health ensures the future health of next generations. The aim of the current study was to evaluate the effect of problem-solving and assertiveness skill training on improving self-esteem and mental health in high school female students of Isfahan city in 2019.

Methods

This study was a randomized clinical trial. The target population was 10th grade female high school students in Isfahan city of Iran. 96 students of a female public high school were entered the study allocated to two groups, 32 for intervention group and 64 for control group. Six 90-minute sessions were considered for problem-solving and assertiveness skill training which included lectures, question and answer, movie displaying, brainstorming and role-play. The Coopersmith Self-esteem Inventory Questionnaire (CSEI) and General Health Questionnaire (GHQ) were used in order to evaluate the variables of the study before and one month after the intervention.

Results

Mean scores of the self-esteem variable changed significantly in the intervention group compared to control group before (25.2 ± 2.905) and after (29.9 ± 4.155) the intervention(p < 0.05). Mean scores of mental health also changed significantly before (27.67 ± 5.42) and after (19.033 ± 4.9) the intervention in comparison with the control group(p < 0.05).

Conclusion

The findings of the present study showed that educational intervention based on problem-solving and assertiveness may enhance self-esteem and mental health in students. Future studies are needed to confirm and determine the structure of these associations.
Trial Registration:
IRCT Code: IRCT20171230038142N9
Registration Date: 07/07/2019
Ethics Code: IR.MUI.MED.REC.1398.130
The impact of long-term care needs on the socioeconomic deprivation of older people and their families: results from a scoping review
Authors: CASANOVA GEORGIA , Martarelli Rossella , Belletti Francesco , Lamura Giovanni 
Published: December 2022

Abstract
Background
Long-term care (LTC), poverty, and socioeconomic deprivation are globally significant social issues. Ongoing population aging trends and the recent social and health emergencies caused by the COVID-19 pandemic crisis have highlighted the need for macro-level LTC and welfare system sustainability strategies. At the micro level, the relationship between LTC needs and the risk of socioeconomic deprivation and poverty should be taken into account to promote more targeted and innovative policies worldwide. This scoping review explores the relationship between LTC needs, the health status of older people, and the risk of socioeconomic deprivation for their families in order to understand how the literature interprets these concepts and the relationship between them and to identify any potential gaps in this regard.
Methods
The methodology considers different relevant sources: a) the guidelines for ScR proposed by Lockwood et al. [1]; b) the Munn et al. [2] recommendations; c) the PRISMA guideline for Scoping Reviews [3]; and d) the Joanna Briggs Institute (JBI) checklist [4]; Sixty-three papers are included in the frequency analysis of 9 identified categories to respond of aims.
Results
The findings reveal the existence of a debate that seeks to understand the different characteristics of the relationship between the investigated issues. Specific targets (such as older people, caregivers, and households) are used to identify LTC needs, while material deprivation or poverty is used to determine the conditions of deprivation. Relevant gaps in the literature are identified in terms of the concepts and approaches of the studies analyzed. The results indicate that the reciprocal relationship between LTC needs, supply, and the risk of socioeconomic deprivation is understudied.
Conclusions
The simplification strategy used in many studies to reduce the relationship’s complexity precludes an in-depth analysis and debate on some relevant aspects, including the crucial two-way relationship between LTC needs/supply and the risk of socioeconomic deprivation. Future studies should focus on the causal relationship between the two phenomena and identify any internal factors that may be involved.
Impacts of natural and anthropogenic forcings on historical and future changes in the global land-surface air temperature simulated by CMIP6–DAMIP
Authors: Xu Chuan , Zhao Tianbao , Zhang Jingpeng , Hua Lijuan , Tao Li 
Published: December 2022

Abstract
Detection and attribution of climate change are essential for understanding past, present, and future climate changes arising from either natural, unforced variability or in response to changes in natural and anthropogenic forcings. In this paper, we investigate the historical change and future projection of the global land-surface air temperature under natural and anthropogenic forcings based on all available model simulations from the Detection and Attribution Model Intercomparison Project (DAMIP) in the sixth phase of the Coupled Model Intercomparison Project (CMIP6). The results show that the increase in the global land-surface air temperature can be largely attributed to human activities. The effect of anthropogenic forcing (ANT) can be robustly detected and separated from the response to the natural external forcing (NAT). Greenhouse gas (GHG) is the primary contributor to anthropogenic climate warming, and the effect of GHG can be clearly detected and successfully attributed to the observed T increases. After re-scaling the T trends, the ANT- and GHG-induced global land warming is in the range of 0.6–1.6 °C/100 yr and 0.8–2.0 °C/100 yr, respectively. In the twenty-first century, GHG is still a decisive factor causing future climate warming under the Medium Shared Socioeconomic Pathways (SSP2-4.5) scenario, especially in the high latitudes of the Northern Hemisphere, causing large increases (decreases) in the frequency and magnitude of hot (cold) extremes in the late twenty-first century.
Does Renewable Energy effect the Environmental Sustainability? Nexus among Renewable Energy Consumption, Trade Openness, CO2 Emissions, Income Inequality and Economic Growth
Authors: Ramos-Meza Carlos Samuel , Arocutipa Javier Pedro Flores , Huallpa Jorge Jinchuña , Palomo Erika Eleana Corzo , Huarcaya Valery Kimiyo Gamero , Acuña Yeni Gutiérrez , Martinez Juan Carlos Valencia 
Published: December 2022

AbstractThis research explores the relationship among renewable energy consumption (REC), trade openness, carbon emanations, income inequality on economic growth. This study takes fifteen (15) OECD nations data set start from 1997 to 2020 by employing the AMG assessor, which takes into account slope heterogeneity (SH) and CSD. The empirical outcomes confirm a significant favorable long-run relationship between these nexus, except for Carbon emanations, which are adversely related with REC, respectively. Notwithstanding, it has discovered that there is bi-directional causality between income inequality and REC.JEL Classification:D47
Effects of higher-frequency out-of-bed mobilization training on the recovery of acute stroke patients: study protocol for a randomized controlled trial
Authors: Yang Qian , Jin Lixia , Fu Tengfei , Shen Qiyuan , Huang Yiming , Ni Jiali , Chen Jun , Liu Bangzhong , Zhang Jian 
Published: December 2022

AbstractBackground Out-of-bed mobilization is a significant component within the rehabilitation of people early after stroke. A top priority in acute stroke rehabilitation research is determining the optimal dose of out-of-bed mobilization exercises, such as frequency and timing. However, there is little evidence of the appropriate frequency out-of-bed mobilization for acute stroke patients. Aims The aim of the study described in this protocol is to investigate the effect of higher-frequency out-of-bed mobilization on the functional recovery of acute stroke patients. Methods This is a prospective randomized three-arm trial with the assessor blinded to the study intervention. 45 patients met the inclusion criteria will be randomly allocated to three groups, which are separately usual care (1 mobilization session/d), Frequency 1 group (2 mobilization sessions/d) and Frequency 2 group (4 mobilization sessions/d). Outcomes: The primary outcome is the modified Rankin Scale and the secondary outcomes are Rivermead Mobility Index, Fugl-Meyer (Lower Limb), Barthel Index and adverse events incidence. Outcomes will be measured at three time points.Summary:The proposed study will be beneficial for determining the frequency of out-of-bed mobilization, filling the gaps in the acute rehabilitation research field and optimising the recovery of people early after stroke.Trial registration: The proposed study has been registered at Chinese Clinical Trial on 11/03/2022, registry ID: ChiCTR2200057404.
Impact of Sexual Intercourse on Frozen-thawed Embryo Transfer Outcomes: A Randomized Controlled Trial
Authors: Hou Jin-Wei , Yuan Li-Hua , Cao Xian-Ling , Song Jing-Yan , Sun Zhen-Gao 
Published: December 2022

Abstract
Background: Exposure of the female reproductive tract to either seminal plasma or fluid component of the ejaculate is beneficial to achieving successful embryo implantation and normal embryo development. But whether the "physical" component of sexual intercourse during the peri-transfer period have any influence on frozen-thawed embryo transfer (FET) pregnancy outcomes is not clear.
Methods: We conducted a randomized trial that included 223 patients undergoing in vitro fertilization (IVF) treatment at a university-affiliated reproductive center from 19 July 2018 to 24 February 2019. Enrolled patients undergoing IVF treatment were randomized either to engage sexual intercourse using the barrier contraception (Group A, n=116) or to abstain (Group B, n=107) one night before FET. The primary outcome was clinical pregnancy rate.
Results: Patients having intercourse had higher clinical pregnancy rate (51.72% vs. 37.07%, P=0.045) and implantation rate (38.31% vs. 24.77%, P=0.005) compared to those did not engage intercourse. However, there was no significant difference of the spontaneous abortion rate between two groups (11.67% 33 vs. 14.63%, P=0.662).
Conclusions: Sexual intercourse before embryo transfer may improve the clinical pregnancy and implantation rates during FET cycles. However, it should be noted that patients choose only one time for sexual intercourse, that is, the night before embryo transfer.
Trial registration: The present study was registered at the Chinese Clinical Trial Registry (http://www.chictr.org.cn/, ChiCTR1800017209).
Neochloris oleoabundans cell wall rupture through melittin peptide: a new approach to increase lipid recovery
Authors: Vargas-Perez Magda , Gonzalez-Horta Azucena , Mendoza-Hernandez Hiram , Elías-Santos Myriam , Acuña-Askar Karim , Galán-Wong Luis Jesús , Luna-Olvera Hugo Alberto 
Published: December 2022

Abstract
Objectives The cell wall of microalgae is a necessary barrier for its survival in aquatic environments. This barrier affects the recovery of lipids, representing one of the main difficulties in the development of microalgal biofuel production technology. In this context, this work aimed to test the effect of a new method based on melittin peptide as a pretreatment to cause cellular disruption in N. oleoabundans cultivated under different temperatures and nutrient concentrations.
Results Neochloris oleoabundans cells were grown at 32°C in the presence of high concentration of nitrate-phosphate, cell disruption degree being recorded at 83.6%. Also, two-fold increase in lipid recovery following melittin treatment and solvent extraction was observed. Furthermore, it was possible to verify the effect of melittin, both before and after treatment on the morphology of the cells. Scanning electron microscopy (SEM) and confocal images of the melittin-treated microalgae revealed extensive cell damage with degradation of the cell wall and release of intracellular material.
Conclusions Melittin peptide produced a selective cell wall rupture effect with the highest percentage of cell disruption degree when N. oleoabundans under culture condition with nitrate-phosphate repletion at 32 °C favors cell wall susceptibility to cell disruption by melittin peptide, representing the first report on lipid recovery from microalgae. Melittin is a promising option for the enhancement of the recovery of lipids from microalgae.
Correlation Analysis of Fall Risk with Body Composition in the Tibetan Population
Authors: chen ying , Wen Youfeng , Xiao Yanjie , Ye Liping , Gao Xiaoping , Yao Jie , Song Jia , Liu Yingying , Li Xin , jiang yaqiong , zidan zidan 
Published: December 2022

Abstract
To understand the risk of falls in the high-altitude population, the skeletal muscle quality of the limbs of 705 Tibetan adults was measured according to the fall risk assessment scale. We found that the detection rate of fall risk in the Tibetan population was 24.5% in Shigatse, Lhasa, and the detection rate of fall risk increased significantly with age. The body composition of the group without fall risk was higher than that of the group with fall risk. The T value and bone strength index of the group without fall risk were higher than those of the group with fall risk, and the fracture risk was lower than that of the group with fall risk.
Horizontally-transferred T-DNA and haplotype-based phylogenetic analysis uncovers the origin of sweetpotato
Authors: Yan Mengxiao , Li Ming , Wang Yunze , Wang Xinyi , Moeinzadeh M-Hossein , Quispe-Huamanquispe Dora , Fan Weijuan , Wang Yuqin , Nie Haozhen , Wang Zhangying , Heider Bettina , Jarret Robert , Kreuze Jan , Gheysen Godelieve , Wang Hongxia , Vingron Martin , Bock Ralph , Yang Jun 
Published: December 2022

Abstract
The hexaploid sweetpotato is one of the most important root crops worldwide. However, its genetic origins remain controversial. In this study, we identified two likely progenitors of sweetpotato by analyzing the horizontally transferred IbT-DNA and a haplotype-based phylogenetic analysis. The diploid form of I. aequatoriensis contributed the B1 subgenome, the IbT-DNA2 and the lineage 2 type of the chloroplast genome to sweetpotato. The tetraploid progenitor of sweetpotato is I. batatas 4x, donating the B2 subgenome, IbT-DNA1 and the lineage 1 type of chloroplast genome. Sweetpotato is derived from reciprocal crosses between the diploid and the tetraploid progenitor, and a subsequent whole genome duplication. We also detected biased gene exchanges between subgenomes. The B1 to B2 subgenome conversions were almost 3-fold higher than the B2 to B1 subgenome conversions. This study sheds lights on the evolution of sweetpotato and paves the way for the improvement of the crop.
Implicit Biases in Refereeing: Lessons from NBA Referees
Authors: Pelechrinis Konstantinos 
Published: December 2022

Abstract
Implicit biases occur automatically and unintentionally and are particularly present when we have to make split second decisions. One such situations appears in refereeing, where referees have to make an instantaneous decision on a potential violation. In this work we revisit and extend some of the existing work on implicit biases in refereeing. In particular, we focus on refereeing in the NBA and examine three different types of implicit bias; (i) home-vs-away bias, (ii) bias towards individual players or teams, and, (iii) racial bias. For our study, we use play-by-play data and data from the Last Two Minutes reports the league office releases for games that were within 5 points in the last 2 minutes since the 2015 season. Our results indicate that the there is a bias towards the home team - particularly pronounced during the playoffs - but it has been reduced since the COVID-19 pandemic. Furthermore, there is robust statistical evidence that specific players benefit from referee decisions more than expected from pure chance. However, we find no evidence of negative bias towards individual players, or towards specific teams. Finally, our analysis on racial bias indicates the absence of any bias.
Mechanical stretching induces fibroblasts apoptosis through activating Piezo1 and then destroying actin cytoskeleton
Authors: Li Yang , Liao Wenxin , Liu Tingting , Shen Fujin , Li Hong 
Published: December 2022

Abstract
Ligaments and muscles maintain the anatomical positions of pelvic floor organs. Stress urinary incontinence (SUI) occurs when pelvic floor tissues are repeatedly stimulated with excessive mechanical tension that is beyond the capacity of ligaments or muscles to endure. In addition, cells respond mechanically to mechanical stimulation by reconstituting the Piezo1 and cytoskeletal system. This study sought to determine how Piezo1 and actin cytoskeletal contribute to MS-induced apoptosis in human anterior vaginal wall fibroblasts (hAVWFs) and the mechanisms involved. A four-point bending device was used to provide mechanical stretching to establish a cellular mechanical damage model . MS significantly induced apoptosis of hAVWFs from non-SUI patients, which exhibited apoptosis rates comparable to those of SUI patients, and silencing of Piezo1 decreased the MS-induced apoptosis. However, the disassembly of actin cytoskeleton suppressed the protective effect of Piezo1 silencing from MS. Based on these findings, Piezo1 links the actin cytoskeleton to apoptosis in hAVWFs, providing insight into the clinical diagnosis and treatment of SUI.
Bacteria-based multiplex system eradicates recurrent infections with drug-resistant bacteria via photothermal killing and protective immunity elicitation
Authors: Zhu Chen , Xu Youcui , Wu Yi , Hu Yi , Xu Mengran , Liu Yanyan , Ding Yuting , Wen Longping , Li Jiabin 
Published: December 2022

Abstract
Background: The high mortality associated with drug-resistant bacterial infections is an intractable clinical problem because of the low susceptibility of the bacteria involved to antibiotics and high incidence of recurrent infections.
Methods: Herein, a photosynthetic bacteria-based multiplex system composed of natural Rhodopseudomonas palustris (Rp) and Food and Drug Administration-approved aluminum adjuvant (Rp@Al), was developed to combat drug-resistant bacterial infections and prevent their recurrence. We examined its photothermal performance, in vitro and in vivo antibacterial ability; revealed its protective immunomodulatory effect; verified its prevention effect on recurrent infections; demonstrated the system safety.
Results: Rp@Al exhibits excellent photothermal properties with an effective elimination of methicillin-resistant Staphylococcus aureus (MRSA). In addition, Rp@Al elicits the activation of dendritic cells and further triggers a T helper 1 (TH1)/TH2 immune response, resulting in pathogen-specific immunological memory against recurrent MRSA infection. Upon second infection, Rp@Al-treated mice show significantly lower bacterial burden, faster abscess recovery, and higher survival under near-lethal infection doses than control mice.
Conclusions: This innovative multiplex system, with superior photothermal and immunomodulatory effects, presents great potential for the treatment and prevention of drug-resistant bacterial infections.
A weak-labelling and deep learning approach for in-focus object segmentation in 3D widefield microscopy
Authors: Li Rui , Kudryashev Mikhail , Yakimovich Artur 
Published: December 2022

Abstract
Three-dimensional information is crucial to our understanding of biological phenomena. The vast majority of biological microscopy specimens are inherently three-dimensional. However, conventional light microscopy is largely geared towards 2D images, while 3D microscopy and image reconstruction remain feasible only with specialized equipment and techniques. Inspired by the working principles of one such technique - confocal microscopy, we propose a novel approach to 3D widefield microscopy reconstruction through semantic segmentation of in-focus and out-of-focus pixels. For this, we explore a number of rule-based algorithms commonly used for software-based autofocusing and apply them to a dataset of widefield focal stacks. We propose a computation scheme allowing the calculation of lateral focus score maps of the slices of each stack using these algorithms. Furthermore, we identify algorithms preferable for obtaining such maps. Finally, to ensure the practicality of our approach, we propose a surrogate model based on a deep neural network, capable of segmenting in-focus pixels from the out-of-focus background in a fast and reliable fashion. The deep-neural-network-based approach allows a major speedup for data processing making it usable for online data processing.