bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing the nutritive value of canola meal for broiler chickens through enzymatic modifications
Authors: Niu Yanxing , Rogiewicz Anna , Patterson Rob , Slominski Bogdan A 
Published: July 2023

Abstract
Enzymatic modification of canola meal (CM) is a potential method to enhance its nutritional value as it can depolymerize nonstarch polysaccharides (NSP) and mitigate its potential antinutritive properties. Based on the previous studies, pectinase A (PA), pectinase B (PB), xylanase B (XB), and invertase (Inv) were used for the enzymatic modifications. The highest NSP depolymerization ratio was obtained when 4 g/kg of each PA, PB, and XB, and 0.2 g/kg of Inv were used during 48 h incubation at 40 °C. In the current study, changes in pH, simple sugars, sucrose, oligosaccharides, and NSP contents during the enzymatic modification (CM+E) of CM were measured and compared to Control (CM) without enzymes addition or with the addition of bacteriostat sodium azide (CM+E+NaN3). The results showed that spontaneous fermentation occurred during incubation. After incubation, the pH of the slurry decreased, lactic acid was produced, phytate disappeared, and the concentration of simple sugars decreased substantially. The NSP of the slurry was progressively depolymerized by the enzyme blend. The chemical composition and nutritive value of enzymatically-modified CM (ECM) were evaluated. Ross 308 broilers were randomly assigned to 18 cages of six birds each for the standardized ileal amino acid digestibility (SIAAD) and nitrogen-corrected apparent metabolizable energy (AMEn) assay. A corn/soybean meal-based basal diet formulated to meet Ross 308 breeder recommendations and two test diets contained 70% of the basal diet and 30% of CM or ECM, respectively, were fed to Ross 308 from 13 to 17 d of age. No difference was observed between SIAAD of CM and ECM. The AMEn value of ECM was 2118.0 kcal/kg on a dry matter basis which was 30.9% greater (P &lt; 0.05) than the CM.
Survival in sporadic ALS is associated with lower p62 burden in the spinal cord
Authors: Pinkerton Monica , Lourenco Guinevere , Pacheco Maria Torres , Halliday Glenda M , Kiernan Matthew C , Tan Rachel H 
Published: July 2023

Abstract
The autophagy marker p62 appears as a consistent component of pathological aggregates in amyotrophic lateral sclerosis (ALS) and its modulation to facilitate protein degradation has been proposed as a potential therapeutic target. Importantly, recent studies have implicated diffuse phosphorylated TDP-43 inclusions that are immuno-negative for p62 in more rapid disease, highlighting the need for better understanding of p62 involvement in ALS pathogenesis. The present study set out to assess p62 pathology in the motor neurons of 31 patients with sporadic ALS that had either a short (&lt;2 years) or longer (4–7 years) disease duration to determine its association with pTDP-43 pathology, motor neuron loss, and survival in sporadic disease. Our results identified significantly more cytoplasmic p62 aggregates in the spinal cord of patients with a shorter survival. Disease duration demonstrated a negative association with p62 burden and density of remaining motor neurons in the spinal cord, suggesting that survival in sporadic ALS is associated with the successful clearance of lower motor neurons with p62 aggregates. These findings implicate the autophagy pathway in ALS survival and provide support for further study of p62 as a potential prognostic biomarker in ALS.
Comparison of Administrative Database-Derived and Hospital-Derived Data for Monitoring Blood Culture Use in the Pediatric Intensive Care Unit
Authors: Xiao Shaoming , Woods-Hill Charlotte Z , Koontz Danielle , Thurm Cary , Richardson Troy , Milstone Aaron M , Colantuoni Elizabeth ,   The Bright STAR Authorship Group, Elaine-Marie Albert J , Zerr Danielle M , Foster Charles B , Ezetendu Chidiebere , Hauger Sarmistha B , Dallefeld Samantha H , Sandora Thomas J , Priebe Gregory P , Tadphale Sachin D , Hysmith Nicholas D , Nolt Dawn , Kirby Aileen L , Toltzis Philip , Clayton Jason A , Agulnik Asya , Wolf Joshua , Newland Jason G , Lin John C , Steffen Katherine M , Schwenk Hayden T , Flaherty Brian F , Larsen Gitte Y , Coffin Susan E , Fainberg Nina A , Linam William M , Smith Craig M , Kociolek Larry K , Hong Sue J , Miller Marlene R , Xie Anping , Fackler James C 
Published: July 2023

Abstract

Background
Optimizing blood culture practices requires monitoring of culture use. Collecting culture data from electronic medical records can be resource intensive. Our objective was to determine whether administrative data could serve as a data source to measure blood culture use in pediatric intensive care units (PICUs).

Methods
Using data from a national diagnostic stewardship collaborative to reduce blood culture use in PICUs, we compared the monthly number of blood cultures and patient-days collected from sites (site-derived) and the Pediatric Health Information System (PHIS, administrative-derived), an administrative data warehouse, for 11 participating sites. The collaborative’s reduction in blood culture use was compared using administrative-derived and site-derived data.

Results
Across all sites and months, the median of the monthly relative blood culture rate (ratio of administrative- to site-derived data) was 0.96 (Q1: 0.77, Q3: 1.24). The administrative-derived data produced an estimate of blood culture reduction over time that was attenuated toward the null compared with site-derived data.

Conclusions
Administrative data on blood culture use from the PHIS database correlates unpredictably with hospital-derived PICU data. The limitations of administrative billing data should be carefully considered before use for ICU-specific data.
Surgical management of deep dermatophytosis in a patient with CARD9 deficiency
Authors: El Maati Maha , Berjaou Zakia , Hammouch Zaineb , Khachani Kenza , Moustakbal Iliass , Meziane Meryem , Ismaili Nadia , Benzekri Laila , Aoufi Sara , Mazouz Samir , Senouci Karima 
Published: July 2023

Abstract
Deep dermatophytosis is a dermal infection caused by Dermatophytes. It can cause deeper dermal dermatophytosis, Majocchi’s granuloma, dermatophytic pseudomycetoma or a widespread infection. CARD9 deficiency is a known risk factor in the Mediterranean region, first reported in 1964 in Morocco. We report a case of 23-year-old man with a scarring alopecia who presented with subcutaneous abscesses topped off with a large ringworm infection. Mycotic analysis revealed a Trichophyton Rubrum deep dermatophytosis. The molecular study revealed a CARD9 mutation confirming dermatophytosis with parotid and lymph nodes involvement. The patient underwent successful drainage surgery of the abscesses alongside medical treatment including antifungal agents and he was discharged after an uneventful postoperative course.
(20) DUAL EFFECT OF A SEROTONIN AND DOPAMINE REUPTAKE INHIBITOR (IP2018), AND THE SEARCH FOR A DEPRESSIVE RAT MODEL WITH ERECTILE DYSFUNCTION
Authors: Simon G Comerma Steffensen , Eugenio Gutierrez , Rafael Fais , Dan Peters , Claus Olesen , Prof. Gregers Wegener , Prof. Ulf Simonsen 
Published: July 2023

Abstract

Objectives
We investigated whether an amine transport inhibitor, IP2018, improves depression and erectile function in the same dose range.

Methods
The dose-dependent effect of IP2018 in mouse models for depression, the mouse-forced swim test (mFST) and mouse-tail suspension test (mTST), is reported. In male Wistar, Sprague Dawley and the depressive rats (Flinders Sensitive Line (FSL) and Flinders Resistant Line (FRL)), increases in intracavernosal pressure (ICP) and mean arterial pressure (MAP) were measured, and the ratio ICP/MAP was used as a measure of erectile function. We investigated whether IP2018 induces changes in ICP/flow and whether IP2018 facilitates suboptimal increases in ICP induced by cavernosal nerve electrical stimulation. The late procedure was performed in the rest of the rats to evaluate their erectile function.

Results
We found that infusion of IP2018 dose-dependently improved mFST and was more potent than citalopram. At the same time, higher doses of IP2018 were required to obtain effect in the mTST model, and IP2018 was equipotent to citalopram. Infusion of low doses of IP2018 dose-dependently increased the number and the duration of spontaneous erections. At the highest dose of IP2018, the increases in the number and duration of spontaneous erections were similar to the effect of the lowest dose, and the increases in flow were 5 times higher. IP2018 (1-30 μM) caused small relaxations in isolated corpus cavernosum strips. FSL and FRL rats showed a diminished ICP/MAP compared to Sprague Dawley control rats.

Conclusions
IP2018 is more potent or has a similar effect to the well-known antidepressant drug citalopram in mouse models for depression. The potent effect of IP2018 on erectile function in anaesthetized rats suggests that IP2018 in the same dose range has positive effects on both depression and erectile function. FSL/FRL rats appear to allow the study of drug effects on both depression and sexual function in one animal model.

Conflicts of Interest
There is no conflict of interest.
(118) THE MECHANISM OF ACTION OF PHOSPHODIESTERASE TYPE 5 INHIBITORS IN PREVENTING MYOFIBROBLAST TRANSFORMATION IN IN VITRO MODELS OF PEYRONIE’S DISEASE
Authors: Sophie L Harding , Dr. Marcus M Ilg , Dr. Alice R Lapthorn , Prof. Selim Cellek , Prof. David J Ralph 
Published: July 2023

Abstract

Objectives
Peyronie’s disease (PD) is a fibrotic disorder of the penis, where a fibrous plaque forms in the tunica albuginea (TA), leading to pain, penile deformity, and erectile dysfunction. There is currently no effective medical treatment available. The transformation of fibroblasts into myofibroblasts is a key event in the pathophysiology of PD and previous work has shown that phosphodiesterase type 5 inhibitors (PDE5i) can prevent this transformation in vitro and prevent PD development in vivo. This study aims to identify the molecular mechanism of the anti-myofibroblast action of PDE5i.

Methods
Primary fibroblasts were isolated from the TA of patients undergoing corrective surgery for PD. Myofibroblast transformation was determined utilizing an In-cell ELISA assay by quantifying the expression of α-smooth muscle actin in response to 10 ng/mL TGF-β1. Concentration response curves (CRCs) were generated to confirm the anti-fibrotic action of PDE5i, and in the presence and absence of pharmacological inhibitors to identify molecular components involved in the mechanism of action of PDE5i. Three biological and three technical replicates were generated, and statistical significance determined using unpaired Students’ t-test.

Results
TGF-β1 receptor inhibitor SB-505124 and PDE5i vardenafil abrogated TGF-β1-induced myofibroblast transformation with an IC50 of 0.29 ± 0.07 μM and 10.15 ± 3.53 μM, respectively. At 1 μM, protein kinase G inhibitor KT5823 did not prevent the antifibrotic action of vardenafil. The cAMP analog 8-Br-cAMP and PDE3i cilostamide abrogated TGF-β1-induced myofibroblast transformation with an IC50 of 6.88 ± 14.29 μM, and 14.05 ± 1.63 μM, respectively. In the presence of 1 μM KT5720, a protein kinase A inhibitor, the antifibrotic effect of vardenafil was significantly reduced.

Conclusions
These results suggest that PDE5i prevent TGF-β1-induced transformation of myofibroblasts not through PKG, but through the PDE3/cAMP/PKA pathway. Although such an interaction has been shown in other fibroblasts, this is the first demonstration in PD fibroblasts.

Conflicts of Interest
No known conflicts of interest.
(201) PENILE PROSTHESIS INSERTION POST-PRIAPISM: SATISFACTION GUARANTEED?
Authors: Dr. Aisling Looney , Katy Naylor , Dr. Chloe Mount , Fiona Holden , Dr. Mark Johnson , Prof. David Ralph , Dr. Philippa Sangster 
Published: July 2023

Abstract

Objectives
Penile prosthesis (PP) insertion is recommended for patients with ischaemic priapism of long duration in order to restore erectile function; but little has been published regarding quality of life (QOL) for those with implants post-priapism. This the largest study to date assessing QOL outcomes in these patients.

Methods
Within our department, 167 patients had a PP inserted following priapism between 2002-2022. We identified 42 patients that had PPs inserted for priapism between 2007-2021. Clinical and demographic details were recorded on an electronic log. Of these 42, 18 (43%) were contactable and agreeable to email or telephone questionnaire. The validated Quality of Life and Sexuality with Penile Prosthesis (QoLSPP) questionnaire was administered and responses were recorded. Two additional questions were asked regarding regret over PP insertion and feelings regarding their future with a PP.

Results
The median time to implant insertion post-priapism was 15 days. Of the 18 patients who responded, 16/18 (89%) were actively using their prosthesis. Half of these patients (9/18) had an inflatable device inserted primarily. The median duration of PP insertion was 36.5 months. No patients (0/18, 0%) regretted their decision to have a PP inserted at the time of priapism, and 13/18 (72%) would be satisfied living the remainder of their life with a PP. Regarding pleasure, 12/18 (67%) of respondents felt their PP was satisfactory half the time or more, and 76% (13/17 responses) felt their PP had met expectations. Of the 16 men who responded regarding partner satisfaction, 10 (63%) felt their partner was satisfied with the PP half the time or more.

Conclusions
PP insertion is a suitable surgical technique to maintain sexual function in select patients post-priapism. We demonstrated that the majority of men who underwent PP insertion post-priapism were satisfied with the results, and all would choose to have a PP inserted were they to return to their priapism event again.

Conflicts of Interest
Nil
(268) EARLY EXPERIENCE IN PENILE REHABILIATION AFTER RADICAL PROSTATECTOMY
Authors: Senad Bajramovic , Prof. Benjamin Kulovac , Prof. Damir Aganovic , Dr. Jasmin Alic , Hajrudin Spahovic , Nirvana Sabanovic Bajramovic 
Published: July 2023

Abstract

Objectives
The incidence rate of erectile dysfunction (ED) is reportedly as high as 30-90% after radical prostatectomy which seriously affects the patients' quality of life. Penile rehabilitation is defined as the use of any drug or device at or after radical prostatectomy to maximize erectile function recovery.

Methods
Prospective study in men who underwent open radical prostatectomy and who received penile rehabilitation after surgery. Highly motivated (group I) patient started treatment with 5 mg tadalafil on the fifth postoperative day and continued to receive every day, regardless of erectile function. Intracavernous injection of prostaglandin E1 was initiated at 1 month. Minimally motivated patients (group II) started PDE5i on demand only. Follow up evaluations were done at 3, 6, 12, 18 and 24 months. Patients filled in International Index of Erectile Function-5 (IIEF-5) questionnaires.

Results
Of 165 men who underwent open radical prostatectomy 48 patients met inclusion criteria, complied with the study medication, and had complete data for 24 months. A total of 12 patients (25%) underwent (undefined include also unilateral) nerve-sparing surgery (NSS), while 36 patients (75%) had bilateral nerve-sparing surgery. At 24 months, 30% of patients who underwent undefined nerve-sparing surgery and 60% of patients who underwent bilateral nerve-sparing surgery reported having sufficient erectile function for intercourse without using intracavernous injection or PDE5i on demand. At 24 months after surgery, the median IIEF-5 score was 13 (1-25) for the whole cohort, 7 (1-25) for patients who had undergone undefined nerve-sparing surgery and 16 (1-25) for patients who had undergone bilateral nerve-sparing surgery. Patients received ICI treatment had success early in the study period compared to patients taking PDE5i alone.

Conclusions
Proper selection of patients, NSS and postoperative counseling as well as patient’s motivations and expectations might explain inferior erectile function results following this surgery.

Conflicts of Interest
No conflicts.
(276) FEASIBILITY OF MINIMALLY INVASIVE, SAME-DAY INJECTION OF AUTOLOGOUS ADIPOSE-DERIVED STEM CELLS IN THE TREATMENT OF ERECTILE DYSFUNCTION
Authors: Prof. Mikkel Fode , Dr. Naomi Nadler , Prof. Lars Lund , Prof. Nessn Azawi 
Published: July 2023

Abstract

Objectives
To investigate feasibility, safety and efficacy of a new minimally invasive same-day method of autologous adipose derived stem cell (ADSC) transplantation in men suffering from erectile dysfunction (ED).

Methods
Prospective case series of 10 men with an IIEF-EF domain score &lt; 17. The IIEF questionnaire was filled out at baseline and 1, 2 and 3 months after treatment. Side effects were assessed by investigations and interviews until 6 months after treatment. The myStem® X2 kit was used for preparation of ADSC: Adipose tissue was harvested from the patient himself under local anesthesia and immediately prepared and injected into the penis. Primary endpoints were feasibility and safety. Secondary outcomes included effects on ED and changes in the remaining IIEF domains.

Results
Ten men were included. Only one adverse event in the form of minor blue discoloration at the fat harvest site was registered. There were statistically significant improvements in IIEF-EF at one, two and three months after treatment compared to baseline with the median score increasing from 5.5 to 10.5, 10.5, and 10 respectively. Considering the individual patients, 3/10 men achieved an improvement equal to or greater than the minimal clinically important difference according to their baseline IIEF-EF score.

Conclusions
Our study confirms the feasibility and safety of this minimally invasive, same-day delivery of ADSC. Due to the design and size on the study, firm conclusions should not be drawn regarding efficacy, but the method seems worthy of further study.

Conflicts of Interest
Mikkel Fode is a speaker for Boston Scientific.
(270) CAN INTRAOPERATIVE NERVE MONITORING IMPROVE FUNCTIONAL OUTCOMES AFTER UNILATERAL- OR NON-NERVE-SPARING ROBOT-ASSISTED RADICAL PROSTATECTOMY?
Authors: Dr. Alexander B Nolsøe , Peter B Østergren , Dr. Henrik Jakobsen , Christian F S Jensen , Dr. Roar Rasmussen , Niels H Bruun , Prof. Jens Sønksen , Prof. Mikkel Fode 
Published: July 2023

Abstract

Objectives
Damage to the pudendal nerve that innervates the external urethral sphincter (EUS) is a factor in the development of urinary incontinence after robot-assisted radical prostatectomy (RARP). We aim to investigate whether nerve identification can prevent nerve damage with a subsequent positive effect on functional outcomes.

Methods
Men scheduled to unilateral- or non-nerve-sparing RARP were randomized (1:1) to standard RARP (CTRL) or intraoperative nerve monitoring (INT) via the PROPEP® system. Two electrodes are inserted in the EUS and the Maryland forceps convey an electric impulse. When a nerve innervating the EUS is located, an action potential is seen on the robot console screen. Subsequently, the area can be spared during dissection.
The primary outcome was the International Consultation on Incontinence Questionnaire (ICIQ) score at 12 months. Secondary endpoints included continence at 12 months defined as &lt;8 grams on a 24-hour pads test or the use of 0 pads per day and the answer “never” to the first ICIQ question regarding incontinence frequency. Also, the international prostate symptom score (IPSS), the Erection Hardness Score (EHS), and sexual activity at 12 months. Outcomes were analyzed using regression with robust variance estimation.

Results
From 2018-2022, 100 men were randomized. A positive nerve signal was found in all the INT-group participants. There were no differences in baseline characteristics between the groups. ICIQ was 5.37 (INT) versus 5.66 (CTRL) (95% CI [-2.57;1.99], p=0.8). Continence was 63.4% (INT) vs. 63.4% (CTRL) (95% CI [-21.43;21.43], p= 1). IPSS score was 5.93 (INT) vs. 5.39 (CTRL) (95% CI [-0.91;1.98], p= 0.46). EHS score was 0.95 (INT) vs. 1.18 (CTRL) (95% CI [-0.73;0.29], p= 0.39). Sexual activity was 61% (INT) vs. 57.5% (CTRL) (95% CI [-18.5;25.5], p= 0.75).

Conclusions
Identification of the somatic nerves that innervate the EUS did not improve functional outcomes compared to standard RARP.

Conflicts of Interest
None of the Authors have any conflicts of interest to report.
(155) THE CLINICAL PROFILE OF MEN WITH PREMATURE EJACULATION AT PRESENTATION HAS CHANGED OVER THE LAST FIFTEEN YEARS: analysis froma longitudinalstudy
Authors: Dr. Luca Boeri , Dr. Edoardo Pozzi , Dr. Federico Belladelli , Dr. Giuseppe Fallara , Dr. Alessandro Bertini , Dr. Simone Cilio , Dr. Francesco Lanzaro , Dr. Luigi Candela , Dr. Christian Corsini , Dr. MAssimiliano Raffo , Ludovica Cella , Fausto Negri , Margherita Fanton , Dr. Paolo Capogrosso , Dr. Alessia Darma , Prof. Francesco Montorsi , Prof. Andrea Salonia 
Published: July 2023

Abstract

Objectives
Patient’s characteristics at first medical evaluation for a specific sexual dysfunction (SD) are strongly correlated to the public awareness towards the specific disease and the availability of novel treatments. We aimed to assess the clinical characteristics of patients presenting for premature ejaculation (PE) as their primary medical complaint over a 15-year time frame

Methods
Data from 257 heterosexual men seeking first medical help for primary PE between 2008 and 2022 were analysed. Clinical data and serum hormones were collected. At baseline all patients completed the IIEF, the Beck Inventory for Depression (BDI), and the Premature Ejaculation Diagnostic Tool (PEDT). Relationship status was categorized as: single vs. stable sexual relationship. Descriptive statistics and linear regression analyses were permformed.

Results
Of all, 182) (71%) patients reported erectile dysfunction (ED) according to IIEF-EF scores. Across the 15-year time frame, patient’s age at presentation significantly decreased over time (p&lt;0.001). Furthermore, depressive symptoms at BDI (p&lt;0.001) and PE severity as scored with the PEDT (p&lt;0.001) linearly decreased. When segregating the whole cohort into 5-year groups, age at presentation decreased from 2008-2012 to 2013-2017 (p=0.04) and further decreased between 2018-2022 (p=0.01). Similarly, baseline BDI scores decreased from 2008-2012 to 2018-2022 (all p&lt;0.001). PE severity at first assessment has decreased since 2017 (p&lt;0.01). Patients more frequently asked for medical attention for PE between 2018-2022 compared to 2013-2017 and 2008-2012 (p&lt;0.001).

Conclusions
This longitudinal study showed that PE patients characteristic at first presentation has significantly changed throughout the last 15 years. Of note, age, PE severity and depressive symptoms showed has decreased overtime. Men in a stable sexual relationship were more likely to seek medical attention for PE in recent years. These results probably reflect the increased public awareness of PE; therefore, patients request PE consultation at younger age, with initial symptoms and with fewer psychological bother related to this condition

Conflicts of Interest
NA