bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of immune microenvironment changes, immune-related pathways and genes in male androgenetic alopecia
Authors: Xiong Hong-Di , Tang Lu-Lu , Chen Hai-Ju , Wu Yi , Li Wen-Yu , Wen Si-Jian , Lin You-Kun 
Published: September 2023

Background:
Although androgenetic alopecia (AGA) is classified as a non-inflammatory alopecia, histological evidence of microinflammation has long been recognized. However, changes in the immune microenvironment, immune-related pathways and the expression of immune-related genes (IRGs) involved in AGA remain unclear.

Methods:
The microarray gene expression data (GSE36169) from patients with male AGA were analyzed. gene set enrichment analysis (GSEA) among statistically changed genes was done. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses among differentially expressed genes were performed. differentially expressed genes were screened to identify IRGs based on the ImmPort database. The cytohubba-MCC plugin of Cytoscape was applied to screen hub immune genes. The infiltration levels of 28 immune cells were quantified adopting single-sample GSEA (ssGSEA) algorithm. The microarray gene expression data (GSE90594) of male AGA was analyzed to validate hub IRGs genes and differential infiltrated immune cells.

Results:
The ssGSEA revealed γδT cell, central memory CD8+ T cell, mast cell, immature B cell, activated CD8+ T cell, effector memory CD4+ T cell, eosinophil and neutrophil were significantly increased infiltration in the bald scalp. GSEA showed statistically changed genes were most enriched in immune related pathways, including innate immune system, adaptive immune system, cytokine signaling, interferon-γ signaling, interferon signaling and interleukins signaling. The 4 hub IRGs, including matrix metallopeptidase 9, protein tyrosine phosphatase receptor type C, bone morphogenetic protein 2, and thrombospondin 1, were enriched in the pathways of allograft rejection, coagulation and interferon-γ response.

Conclusion:
In summary, we proposed that the increase in γδ T cells, central memory CD8+ T cells, activated CD8+ T cell as well as the infiltration of mast cells contributed to immune microenvironment changes in male AGA. The 4 hub IRGs may be involved in the development and progression of hair loss in male AGA through interferon-γ signal pathways.
Correction of severe adult idiopathic scoliosis posteriorly with skipped pedicle screw instrumentation and rod augmentation
Authors: Yanik Hakan Serhat , Ketenci Ismail Emre 
Published: September 2023

Background:
Although it is challenging to correct severe adult idiopathic scoliosis (ADIS) deformities, optimal results can be achieved by multiple asymmetric Ponte osteotomies with lower surgical risks. Skipping the apical instrumentation and using multiple rods and connectors may further facilitate the procedure. We named this method as “modular correction technique” (MCT).

Methods:
Sixty-two patients with severe ADIS who were treated with MCT were recruited into this study, and retrospectively evaluated. Radiographic and functional outcomes as well as pulmonary functions were examined preoperatively and at last follow-up. Main radiologic parameters were related to Cobb angles, coronal, sagittal, and shoulder balance. Scoliosis Research Society-22 questionnaire and the Oswestry Disability Index were used to evaluate clinical outcomes.

Results:
Average age of the patients was 41.3 years (range: 23–65). Thirty-nine of the patients were female and 23 of them were male. Coronal Cobb angle of the main curve, shoulder balance, coronal, and sagittal balance significantly improved after the surgery. Forced vital capacity and forced expiratory volume in the first second were mildly improved after the surgery, however the improvement was not statistically significant. Postoperative clinical outcome scores improved significantly.

Conclusion:
MCT can be performed with low risk of complications and relatively low operation time and blood loss. It facilitates rod insertion and correction maneuvers. Radiologic, especially coronal balance, and functional outcomes can be improved with minimal morbidity while deterioration of pulmonary function is prevented. This technique is useful in severe but relatively flexible ADIS deformities.
Diagnostic journey of chronic eosinophilic pneumonia masked as lung cancer: a rare case report
Authors: Katwal Shailendra , Lamsal Sarita , Bhandari Sushmita , Khanal Shital 
Published: September 2023

Introduction and Importance:
Chronic eosinophilic pneumonia (CEP) is an idiopathic condition characterized by unusually high eosinophil infiltration in the lungs’ interstitium and alveolar spaces. It is extremely rare, accounting for fewer than 3% of all interstitial lung diseases. CEP is frequently misdiagnosed as lung cancer, which can have catastrophic consequences for sufferers. When assessing patients with lung disease, doctors should be aware of CEP’s symptoms and take its prognosis into account because it is a curable disorder.

Case Presentation:
A 40-year-old female presented in the outpatient department of gynecology with a history of abnormal vaginal bleeding for 3 months and mild shortness of breath without any other significant medical history or being under any medications. Physical examination findings were not significant.

Clinical Discussion:
Ultrasound revealed adenomyosis and a hysterectomy was planned. Chest radiograph revealed lung mass and computed tomography scan showed a well-defined mass with a pleural-based nodule. Histopathology revealed interstitial fibrosis and eosinophilic microabscesses. CEP was diagnosed and oral prednisolone was started with a 0.5 mg/kg/day dose. Chest radiographic abnormalities resolved after one month of treatment. Currently, she is asymptomatic.

Conclusion:
Early recognition and diagnosis of lung masses are essential for prompt treatment with corticosteroids. CEP can mimic lung malignancy and should be considered in patients with related symptoms.
The physiological effect of prone positioning and lateral decubitus in non-intubated patients with severe COVID-19: a prospective cohort study
Authors: Siswanto  , Utama Oktaviarum Slamet , Adistyawan Guritno , Sujalmo Purwadi , Tunggadewi Gusti Putri Devany , Shafa Primavera Nabila , Rohman Taufiq , Agustiningsih Denny 
Published: September 2023

Introduction:
Prone positioning is one type of postural lung recruitment manoeuvre that has been widely studied regarding the benefits and physiological changes of the cardiorespiratory system. However, prone positioning is not very comfortable for the patients because they have to lie on their stomachs for a while when the oxygen therapy and other monitoring devices are still attached. Based on patient observations, some patients will change to more comfortable positions, namely using the right/left lateral decubitus position. The purpose of this research was to examine the physiological impact of prone and lateral decubitus position in non-intubated patients who were diagnosed with severe COVID-19.

Methods:
It was a prospective cohort study in subsequent people with severe COVID-19 who obtained a non-rebreathing mask or high-flow nasal cannula at a University Hospital between 1 June and 10 September 2021. The study lasted for a total of 2021 days and involved 48 patients. The patients assumed the prone positioning or lateral decubitus every morning and afternoon for 4 h and were measured for cardiorespiratory parameters and blood gas analysis. It has been reported in line with the STROCSS criteria.

Results:
Dynamic changes based on cardiorespiratory parameters and blood gas analysis parameters in patients with prone and lateral decubitus position in patients with severe COVID-19 receiving conventional oxygen therapy (via non-rebreathing mask) or high-flow nasal cannula did not show any significant difference.

Conclusion:
The physiological effect of prone positioning and lateral decubitus in non-intubated patients with severe COVID-19 are similar. Accordingly, lateral decubitus can be an alternative for postural lung recruitment manoeuvres and warrants further randomized trials.
The ELEVATE Study
Authors: Quaid Melissa L. 
Published: September 2023

OBJECTIVE
The ELEVATE study evaluated whether an educational workshop would improve the perceptions of shared decision-making of nurses in an organization as demonstrated by an increase in Index of Professional Nursing Governance (IPNG) score.

BACKGROUND
Available literature insufficiently links educational interventions to improvements in shared professional governance.

METHODS
Six hundred ninety-one participants' IPNG scores were analyzed before and after an educational workshop was administered to formal administrative leaders and registered nurse (RN) RN council leaders every 6 months during a 2-year research study.

RESULTS
Index of Professional Nursing Governance scores were higher for RNs participating in nursing councils than scores for those who did not. The organization's IPNG scores remained stable from 2019 to 2021. Secondary analysis may suggest that council members were more resilient during crisis.

CONCLUSIONS
Further investigation is needed researching the relationship between professional governance education and RNs' perception of shared decision making.
HLA Alleles in a Behçet Disease Multiethnic Population With and Without Ophthalmic Manifestations
Authors: de Andrade Francisco Assis , Porto Luís Cristóvão , Gomes Ochtrop Manuella Lima , Bacchiega Ana Beatriz Santos , de Almeida Neves Ricardo , Morette Leonardo , Santos Gilson , de Souza Alexandre Wagner Silva , Matos Kimble , Abramino Levy Roger 
Published: September 2023

Objective
The aim of this study was to analyze HLA alleles in patients with Behçet disease (BD) and their correlation with ophthalmic manifestations (OMs) in a multiethnic Brazilian population.

Methods
This case-control study compared 72 BD patients with or without OM who underwent a thorough ophthalmologic evaluation, including best-corrected visual acuity, bino-ophthalmoscopy, and HLA analysis, with 144 matched healthy controls. Fluorescein angiography was also performed in the patients with BD and OM. HLA class I (A, B, and C) and II (DRB1, DQB1, and DQA1) typing were performed using PCR-SSO.

Results
Of 72 patients with BD, 42 (58%) had OM. The HLA-B*51 and -A*26 alleles were more frequent in patients with BD than in controls (23.6% vs 14.6% and 12.5% vs 4.3%, respectively), but could not differentiate OM risk. The HLA alleles of BD patients that differentiated those with and without OM were HLA-B*15 (40.5% vs 20.7%; odds ratio [OR], 2.59; p = 0.0059), HLA-C*02 (33.3% vs 13.4%; OR, 3.20; p = 0.0024), and HLA-DQB1*03 (64.3% vs 45.7%, p = 0.017), whereas HLA-A*03 (0.0% vs 13.3%, p = 0.006) and HLA-DRB1*15 (4.8% vs 19.5%; OR, 0.21; p = 0.0121) were protective against OM.

Conclusions
In this study of a Brazilian multiethnic BD population, alleles were similar between groups of BD patients with and without OM. We described HLA-B*15, -C*02, and -DQB1*03 as risk factors and -A*03 and -DRB1*15 as protective factors for OM in BD, which could function as biomarkers for predicting disease phenotypes.
Emergency Department and Inpatient Utilization Reductions and Cost Savings Associated with Trauma Center Mental Health Intervention
Authors: Prater Laura , Bulger Eileen , Maier Ronald V. , Goldstein Evan , Thomas Peter , Russo Joan , Wang Jin , Engstrom Allison , Abu Khadija , Whiteside Lauren , Knutzen Tanya , Iles-Shih Matt , Heagerty Patrick , Zatzick Doug 
Published: September 2023

Objective:
The American College of Surgeons Committee on Trauma (ACS/COT) now requires that trauma centers have in place protocols to identify and refer patients at high risk for the psychological sequelae of traumatic injury. No investigations have documented reductions in utilization and associated potential cost savings associated with trauma center mental health interventions.

Summary Background Data:
The investigation was a randomized clinical trial analysis that incorporated novel 5-year emergency department/inpatient health service utilization follow-up data.

Methods:
Patients were randomized to a mental health intervention, targeting the psychological sequalae of traumatic injury (n= 85) versus enhanced usual care control (n= 86) conditions. The intervention included case management that coordinated trauma center-to-community care linkages, psychotropic medication consultation, and psychotherapy elements. Mixed model regression was used to assess intervention and control group utilization differences over time. An economic analysis was also conducted.

Results:
Over the course of the 5 years intervention patients demonstrated significant reductions in emergency department/inpatient utilization when compared to control patients (F [19,3210] =2.23, P =0.009]. Intervention utilization reductions were greatest at the 3-6 month (intervention 15.5% versus control 26.7%, Relative Risk [RR]=0.58, 95% Confidence Interval [95% CI] 0.34, 1.00) and 12-15 month (intervention 16.5% versus control 30.6%, RR=0.54, 95% CI 0.32, 0.91) post-injury time points. The economic analysis suggested potential intervention cost savings.

Conclusions:
Mental health intervention is associated with significant reductions in emergency department and inpatient utilization as well as potential cost savings. These findings could be productively integrated into future ACS/COT policy discussions.
Short Postoperative Intravenous Versus Oral Antibacterial Therapy in Complicated Acute Appendicitis – A Pilot Non-Inferiority Randomized Trial
Authors: Lipping Edgar , Saar Sten , Reinsoo Arvo , Bahhir Artjom , Kirsimägi Ülle , Lepner Urmas , Talving Peep 
Published: September 2023

Objective:
The purpose of this study is to investigate noninferiority of postoperative oral administration of antibiotics in complicated appendicitis.

Background:
Recent investigations have used exclusively intravenous administration of antibiotics when comparing outcomes of postoperative antibacterial therapy in complicated appendicitis. We hypothesized that oral antibacterial treatment results in noninferior outcomes in terms of postoperative infectious complications as intravenous treatment.

Methods:
In this pilot, open-label, prospective randomized trial, all consecutive adult patients with complicated appendicitis including gangrenous appendicitis, perforated appendicitis, and appendicitis with periappendicular abscess between November 2020 and January 2023 were randomly allocated to 24-hour intravenous administration of antibiotics versus 24-hour oral administration of antibiotics after appendectomy. Primary outcomes included 30-day postoperative complications per Comprehensive Complication Index (CCI). Secondary outcome was hospital length of stay (HLOS). Follow-up analysis at 30 days was conducted per intention to treat and per protocol. The study was registered at ClinicalTrials.gov (NCT04947748).

Results:
A total of 104 patients were enrolled with 51 and 53 cases allocated to the 24-hour intravenous and the 24-hour oral treatment group, respectively. Demographic profile and disease severity score for acute appendicitis (DSS) were similar between the study groups. There were no significant differences between the study groups in terms of 30-day postoperative complications. Median CCI did not differ between the study groups. HLOS was similar in both groups.

Conclusions:
In the current pilot randomized controlled trial, the 24-hour oral antibiotic administration resulted in noninferior outcomes when compared to 24-hour intravenous administration of antibiotics after laparoscopic appendectomy in complicated appendicitis.
Neuroleptic Malignant Syndrome in Patients With Dementia: Experiences of A Single Memory Clinic
Authors: Isik Ahmet Turan , Kaya Derya , Ontan Mehmet Selman , Mutlay Feyza , Bulut Esra Ates , Dost Fatma Sena , Erken Neziha , Aydin Ali Ekrem 
Published: September 2023

Objectives
Neuroleptic malignant syndrome (NMS) is a life-threatening condition that occurs as an adverse reaction to antipsychotic and antiemetic agents or sudden withdrawal of dopaminergic medications. Given the metabolic and functional reserves and the comorbidities in older adults, NMS may show an atypical course.

Methods
The medical records of patients with neurodegenerative diseases leading to dementia between 2013 and 2020 were reviewed for the diagnosis of NMS. Demographic and clinical characteristics of the patients were obtained from the records of laboratory parameters, management, and length of stay.

Results
Fifteen older adults (19 episodes) diagnosed with NMS were included. The median age was 76 years, and 5 were female. Ten of 15 NMS patients were atypical. Most of them had an infection accompanying NMS. Neuroleptic malignant syndrome was caused by antidopaminergic agents (5 antipsychotics, 1 metoclopramide) in 6 episodes and discontinuation of a dopaminergic agent, l-DOPA, in 12 episodes. In 1 patient, it was associated with simultaneous use of domperidone and amantadine withdrawal. Rigidity in NMS due to l-DOPA discontinuation was higher than in those due to antipsychotic use (P = 0.027). Two of our patients needed intensive care, and 1 died.

Conclusions
This study highlights the high frequency of atypical NMS and the importance of early recognition of this potentially fatal syndrome, which can accompany neurodegenerative diseases and infections in older adults.
Protocol for the development of a tool (INSPECT-SR) to identify problematic randomised controlled trials in systematic reviews of health interventions
Authors: Wilkinson Jack , Heal Calvin , Antoniou George A. , Flemyng Ella , Alfirevic Zarko , Avenell Alison , Barbour Virginia , Brown Nicholas J L , Carlisle John , Clarke Mike , Dicker Patrick , Dumville Jo , Grey Andrew , Grohmann Steph , Gurrin Lyle C , Hayden Jill A , Heathers James , Hunter Kylie E , Lasserson Toby , Lam Emily , Lensen Sarah , Li Tianjing , Li Wentao , Loder Elizabeth , Lundh Andreas , Meyerowitz-Katz Gideon , Mol Ben W , O’Connell Neil E , Parker Lisa , Redman Barbara K. , Seidler Anna Lene , Sheldrick Kyle A , Sydenham Emma , Torgerson David J , van Wely Madelon , Wang Rui , Bero Lisa , Kirkham Jamie J 
Published: September 2023

AbstractIntroductionRandomised controlled trials (RCTs) inform healthcare decisions. It is now apparent that some published RCTs contain false data and some appear to have been entirely fabricated. Systematic reviews are performed to identify and synthesise all RCTs that have been conducted on a given topic. While it is usual to assess methodological features of the RCTs in the process of undertaking a systematic review, it is not usual to consider whether the RCTs contain false data. Studies containing false data therefore go unnoticed and contribute to systematic review conclusions. The INSPECT-SR project will develop a tool to assess the trustworthiness of RCTs in systematic reviews of healthcare related interventions.Methods and analysisThe INSPECT-SR tool will be developed using expert consensus in combination with empirical evidence, over five stages: 1) a survey of experts to assemble a comprehensive list of checks for detecting problematic RCTs, 2) an evaluation of the feasibility and impact of applying the checks to systematic reviews, 3) a Delphi survey to determine which of the checks are supported by expert consensus, culminating in 4) a consensus meeting to select checks to be included in a draft tool and to determine its format, 5) prospective testing of the draft tool in the production of new health systematic reviews, to allow refinement based on user feedback. We anticipate that the INSPECT-SR tool will help researchers to identify problematic studies, and will help patients by protecting them from the influence of false data on their healthcare.
Microstructure-Informed Myelin Mapping (MIMM) from Gradient Echo MRI using Stochastic Matching Pursuit
Authors: Şişman Mert , Nguyen Thanh D. , Roberts Alexandra G. , Romano Dominick J. , Dimov Alexey V. , Kovanlikaya Ilhami , Spincemaille Pascal , Wang Yi 
Published: September 2023

AbstractQuantification of the myelin content of the white matter is important for studying demyelination in neurodegenerative diseases such as Multiple Sclerosis (MS), particularly for longitudinal monitoring. A novel noninvasive MRI method, called Microstructure-Informed Myelin Mapping (MIMM), is developed to quantify the myelin volume fraction (MVF) by utilizing a multi gradient echo sequence (mGRE) and a detailed biophysical model of tissue microstructure. Myelin is modeled as anisotropic negative susceptibility source based on the Hollow Cylindrical Fiber Model (HCFM), and iron as isotropic positive susceptibility source in the extracellular region. Voxels with a range of biophysical parameters are simulated to create a dictionary of MR echo time magnitude signals and total susceptibility values. MRI signals measured using a mGRE sequence are then matched voxel-by-voxel to the created dictionary to obtain the spatial distributions of myelin and iron. Three different MIMM versions are presented to deal with the fiber orientation dependent susceptibility effects of the myelin sheaths: a basic variation, which assumes fiber orientation is an unknown to fit, two orientation informed variations, which assume the fiber orientation distribution is available either from a separate diffusion tensor imaging (DTI) acquisition or from a DTI atlas based fiber orientation map. While all showed a significant linear correlation with the reference method based on T2-relaxometry (p < 0.0001), DTI orientation informed and atlas orientation informed variations reduced overestimation at white matter tracts compared to the basic variation. Finally, the implications and usefulness of attaining an additional iron susceptibility distribution map are discussed.Highlightsnovel stochastic matching pursuit algorithm called microstructure-informed myelin mapping (MIMM) is developed to quantify Myelin Volume Fraction (MVF) using Magnetic Resonance Imaging (MRI) and microstructural modeling.utilizes a detailed biophysical model to capture the susceptibility effects on both magnitude and phase to quantify myelin and iron.matter fiber orientation effects are considered for the improved MVF quantification in the major fiber tracts.acquired myelin and iron maps may be utilized to monitor longitudinal disease progress.
WITHDRAWN: Light affects the prefrontal cortex via intrinsically photosensitive retinal ganglion cells
Authors: Lazzerini-Ospri Lorenzo , Zhan Jesse J. , Thomsen Michael B. , Wang Hui , Messanvi Fany , du Hoffmann Johann , Cravedi Kevin , Komal Ruchi , Chudasama Yogita , Zhao Haiqing , Hattar Samer 
Published: September 2023

Withdrawal StatementThis manuscript has been withdrawn by bioRxiv following a formal request by the NIH Intramural Research Integrity Office owing to lack of author consent.