bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of signal transducer and activator of transcription 3 in osteoblasts impaired the bone healing in inflammatory microenvironment
Authors: Feng Jingyi , Huang Zijing , Lu Jiarui , Chan Laiting , Feng Xin , Lei Lizhen , Huang Zhuwei , Lin Lichieh , Yao Yichen , Zhang Xiaolei 
Published: June 2023

AbstractIntroductionThis study aimed to investigate the effect of Stat3 on the osteoblast‐mediated bone healing in the inflammatory lesion.MethodsThe conditional knockout of Stat3 in osteoblasts (Stat3 CKO) was generated via the Cre‐loxP recombination system using Osterix‐Cre transgenic mice. The calvarial bone inflammatory lesions were established on both Stat3 CKO and wild‐type mice, then harvested to assess the bone healing. In response to lipopolysaccharide (LPS) stimulation, osteoblasts from Stat3 CKO and wild‐type mice were subjected to examine the formation of calcium deposits, the expression of osteogenic markers (i.e., Runx2, OPN, COL1A1), and osteoclast‐related markers (i.e., RANKL, OPG). The EdU and transwell assays were performed to assess the proliferation and migration of the cells.ResultsA decrease in bone mass and an increase in osteolysis were found in the inflammatory lesions on Stat3 CKO mice when compared with the control. More osteoclastic‐like cells and an increased expression of RANKL were observed in Stat3 CKO mice. Both mRNA and protein expressions of Stat3 and osteogenic markers in the lesions were significantly decreased in Stat3 CKO mice. After co‐cultured with osteogenic medium, the Stat3‐deficient osteoblasts were found with a significant decrease in calcium deposits and the expression of osteogenic markers, and with a significant increased expression of RANKL. The impaired ossification of Stat3‐deficient osteoblasts was even more pronounced with the presence of lipopolysaccharides in vitro. The most decrease in cell proliferation and migration was found in Stat3‐deficient osteoblasts in response to LPS.ConclusionsLoss of Stat3 in osteoblasts impaired bone healing in an inflammatory microenvironment.
Operative Effect Comparison of Flexible Drill Guiding vs. Traditional Drill Guiding Template for Lower Cervical Pedicle Screw Insertion: A Retrospective Analysis
Authors: Wu Chao , Deng Jiayan , Hu Haigang , Shen Danwei , Qin Binwei , Wang Xiangyu , Gao Tao , Xu Lian 
Published: June 2023

ObjectiveAccurately inserting pedicle screws is the key point of posterior pedicle screw fixation for lower cervical spine (C3–C7) instability. 3D printing technology can improve the accuracy of screw placement. This study compared the safety of 3D‐printed flexible drill guiding template vs. traditional rigid drill guiding template for lower cervical pedicle screw insertion.MethodsThis was a retrospective study. A total of 34 patients who underwent lower cervical pedicle screw fixation from March 2018 to May 2021 were enrolled in this study, and they were divided into the flexible drill flexible drill group and the traditional drill group. A total of 18 patients in the flexible drill flexible drill group underwent pedicle screw fixation assisted by 3D printed flexible drill guiding templates for the lower cervix, and 16 patients in the traditional drill group underwent pedicle screw fixation assisted by 3D printed regular drill guiding templates for the lower cervix. The length of the incision and intraoperative blood loss during surgery were recorded and compared for the two groups. The grade, deviation of the screw entry point, deviation of the screw medial angle and screw length were measured and compared after surgery for the two groups by independent‐sample tests.ResultsThere was a significant difference in the length of the incision and blood loss between the two groups (P < 0.05). There was a significant difference between the two groups for grade (P = 0.016). The deviation of the screw entry point was 0.65 ± 0.50 mm in the flexible drill group and 0.78 ± 0.83 mm in the traditional drill group. The deviation of the screw medial angle was 2.14 ± 1.78 in the flexible drill group and 4.23 ± 2.51 in the traditional drill group, with a significant difference between the two groups (P < 0.05).ConclusionCompared with regular guiding techniques, lower cervical pedicle screw placement assisted by multistep navigation templates and flexible K‐wires results in less trauma and better safety.
Baseline natriuretic peptides as a predictor of atrial fibrillation recurrence after radiofrequency‐based pulmonary vein isolation in a non‐heart failure population: A subanalysis from DECAAF II
Authors: Younes Hadi , Mekhael Mario , Feng Han , Noujaim Charbel , Chouman Nour , Assaf Ala , Hajjar Abdel‐Hadi el , Ayoub Tarek , Dagher Lilas , Lim Chanho , Pandey Amitabh , Kreidieh Omar , Marrouche Nassir , Donnellan Eoin 
Published: June 2023

AbstractBackgroundBrain natriuretic peptide (BNP) is a marker of myocardial stretch and may have prognostic significance in patients with atrial fibrillation (AF) without heart failure (HF). We investigated the association between baseline BNP levels and arrhythmia recurrence following pulmonary vein isolation (PVI) among patients with persistent AF without HF.MethodsWe analyzed 125 patients with persistent AF without HF who had baseline BNP measured from the DECAAF II trial. The primary outcome was arrhythmia recurrence following ablation. The baseline characteristics across the two groups were compared using Chi‐square test and Wilcoxon rank test accordingly. Cox regression analysis was used to analyze the association between baseline BNP levels and the primary outcome.ResultsAcross the entire cohort, 64 (51%) patients experienced arrhythmia recurrence. When comparing patients who experienced arrythmia recurrence to patients who did not, patients with recurrent arrhythmia had higher levels of pre‐ablation BNP, as evidenced by differences in means (330.05 pg/mL) compared to patients without recurrent arrhythmia (182.39 pg/mL) (p < .05). A cut‐off BNP value of 300 pg/mL provided the largest area under curve (AUC) of receiver‐operating characteristic (ROC) curve on univariate logistic regression. On unadjusted Cox analysis, for every 100 unit increase in BNP, the hazard ratio for the primary outcome increased 1.09 (1.026–1.158) times (p = .004). After adjusting for sex, hypertension, and stroke, the results remained significant (HR = 1.8516, CI 95% [1.0139 – 3.381], p = .045).ConclusionIn the non‐heart failure population, BNP levels predict AF recurrence following PVI in persistent AF patients.
Single‐cell RNA sequencing profiles reveal cell type‐specific transcriptional regulation networks conditioning fungal invasion in maize roots
Authors: Cao Yanyong , Ma Juan , Han Shengbo , Hou Mengwei , Wei Xun , Zhang Xingrui , Zhang Zhanyuan J. , Sun Suli , Ku Lixia , Tang Jihua , Dong Zhenying , Zhu Zhendong , Wang Xiaoming , Zhou Xiaoxiao , Zhang Lili , Li Xiangdong , Long Yan , Wan Xiangyuan , Duan Canxing 
Published: June 2023

SummaryStalk rot caused by Fusarium verticillioides (Fv) is one of the most destructive diseases in maize production. The defence response of root system to Fv invasion is important for plant growth and development. Dissection of root cell type‐specific response to Fv infection and its underlying transcription regulatory networks will aid in understanding the defence mechanism of maize roots to Fv invasion. Here, we reported the transcriptomes of 29 217 single cells derived from root tips of two maize inbred lines inoculated with Fv and mock condition, and identified seven major cell types with 21 transcriptionally distinct cell clusters. Through the weighted gene co‐expression network analysis, we identified 12 Fv‐responsive regulatory modules from 4049 differentially expressed genes (DEGs) that were activated or repressed by Fv infection in these seven cell types. Using a machining‐learning approach, we constructed six cell type‐specific immune regulatory networks by integrating Fv‐induced DEGs from the cell type‐specific transcriptomes, 16 known maize disease‐resistant genes, five experimentally validated genes (ZmWOX5b, ZmPIN1a, ZmPAL6, ZmCCoAOMT2, and ZmCOMT), and 42 QTL or QTN predicted genes that are associated with Fv resistance. Taken together, this study provides not only a global view of maize cell fate determination during root development but also insights into the immune regulatory networks in major cell types of maize root tips at single‐cell resolution, thus laying the foundation for dissecting molecular mechanisms underlying disease resistance in maize.
De se names
Authors: Ezcurdia Maite , Merino‐Rajme Carla 
Published: June 2023

AbstractWe argue that there are names with de se contents and that they are theoretically fruitful. De se names serve to challenge intuitive and otherwise plausible orthodoxies such as Stalnaker's view of communication and Bayesian views of belief update. These implications are also significant for those already sympathetic to the irreducibility of de se content.
Improvement of soil acidification and ammonium nitrogen content in tea plantations by long‐term use of organic fertilizer
Authors: Ye J. , Wang Y. , Wang Y. , Hong L. , Kang J. , Jia Y. , Li M. , Chen Y. , Wu Z. , Wang H. 
Published: June 2023

Abstract
Soil acidification is common in some Chinese tea plantations, which seriously affected growth of tea trees. Hence, it is essential to explore soil remediation in acidified tea plantations for sustainable development of the tea industry. We sought to determine how different fertilizers affect acidified soil and their N transformation in tea plantations.
Different fertilizers were used on acidified tea plantation soils for 4 years (2017–2021), and changes in soil pH, indices related to soil N transformation and tea yield were analysed to construct interaction networks of these indices and find which had the largest influence on fertilization.
Long‐term use of sheep manure reduced soil acidification, increased soil pH, enhanced the number and intensity of N‐fixing and ammonifying bacteria, urease, protease, asparaginase and N‐acetamide glucose ribosidase activity and nifH gene expression. This treatment reduced the number and intensity of soil nitrifying and denitrifying bacteria, nitrate reductase and nitrite reductase activity, while the expression of amoA‐AOA, nirK, nirS, narG and nosZ in turn increased ammonium N content of the soil, reduced nitrate N content, and enhanced tea yield. Topsis index weight analysis showed that ammonium N content in the soil had the largest impact among fertilization effects.
Long‐term use of sheep manure was beneficial in restoring the balance of the micro‐ecosystem in acidified soil. This study provides an important practical basis for soil remediation and fertilizer management in acidified tea plantation soils.
Trichoderma gamsiiT6085, a biocontrol agent of Fusarium head blight, modulates biocontrol‐relevant defence genes expression in wheat
Authors: Risoli Samuele , Petrucci Arianna , Vicente Isabel , Sarrocco Sabrina 
Published: June 2023

AbstractTo enhance the framework of the mechanisms of action used by Trichoderma gamsii T6085 for the control of Fusarium head blight (FHB), this work investigated its ability to modulate the expression of defence‐related genes of wheat (Triticum aestivum ‘Apogee’) in response to endophytic colonization of plant tissues. Changes in relative expression of pal1, pr1, pgip2 and lox1 genes were assessed over time in wheat roots, in spikes colonized by T6085 alone and both T6085 and Fusarium graminearum, and in leaves from wheat seedlings root‐inoculated with T6085. Results indicate the ability of T6085 to induce local and systemic defence responses in wheat plants in the presence of one of the causal agents of FHB. There was a general significant up‐regulation of the plant defence‐related genes analysed, especially in the first days after the application of T6085. According to these results, modulation of plant defence genes could be included within the arsenal of mechanisms used by T6085 when applied to wheat, an additional feature of interest in the management of FHB. To evaluate the effect of the plant genotype on the ability of T6085 to endophytically colonize roots, root colonization was assessed on four cultivars of T. aestivum and two cultivars of T. durum. Data showed that roots of only two T. aestivum cultivars were endophytically colonized by T6085, similar to cv. Apogee used here as control, thus demonstrating an effect of the host genotype on the endophytic ability of T6085.
How to avoid intraoperative and postoperative complications in maxillary sinus elevation
Authors: Testori Tiziano , Tavelli Lorenzo , Scaini Riccardo , Saibene Alberto Maria , Felisati Giovanni , Barootchi Shayan , Decker Ann Marie , Deflorian Matteo Antonio , Rosano Gabriele , Wallace Stephen S. , Zucchelli Giovanni , Francetti Luca , Wang Hom‐Lay 
Published: June 2023

AbstractMaxillary sinus floor elevation, via the lateral approach, is one of the most predictable bone augmentation procedures performed in implant dentistry. but both intra‑ and postoperative complications can occur, and some of them are severe. Our aim is as follows:

To review the pertinent literature on the topic, especially assessing the risk factors related to complications.
To give clinical recommendations to minimize intra‑ and postoperative complications with the ultimate scope of improving the standard of clinical care and patient safety.
Herpesvirus‐Bacteria pathogenic interaction in juvenile (aggressive) periodontitis. A novel etiologic concept of the disease
Authors: Slots Jørgen , Rams Thomas E. 
Published: June 2023

AbstractLocalized juvenile (aggressive) periodontitis starts at puberty in otherwise healthy individuals and involves the proximal surfaces of permanent incisors and first molars. The disease destroys a sizeable amount of periodontal bone within a few months despite minimal dental plaque and gingival tissue inflammation. Cytomegalovirus and Epstein–Barr virus, as well as the two main periodontopathic bacteria Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, are linked to juvenile periodontitis. Juvenile periodontitis‐affected teeth show cementum hypoplasia. We hypothesize that an active herpesvirus infection, at the time of root formation, hampers cementum formation and, at puberty, herpesvirus reactivation triggers an upgrowth of bacterial pathogens which produce rapid periodontal destruction on teeth with a defective periodontium. A pathogenic interaction between active herpesviruses and bacterial pathogens can potentially explain the etiology and incisor‐first molar destructive pattern of juvenile periodontitis. Effective treatment of juvenile periodontitis may target the herpesvirus‐bacteria co‐infection.
Exploring public risk perceptions of microplastics: Findings from a cross‐national qualitative interview study among German and Italian citizens
Authors: Janzik Robin , Koch Severine , Zamariola Giorgia , Vrbos Domagoj , White Mathew P. , Pahl Sabine , Berger Natalie 
Published: June 2023

AbstractMicroplastics are receiving growing attention in the public debate, while the scientific assessment of risks of microplastics to ecological and human health is still ongoing. Previous studies suggest concerns among the general public with country‐specific differences. However, little is known about the reasoning underlying these concerns. By conducting qualitative interviews with German (n = 15) and Italian citizens (n = 15), this study adopted a cross‐national perspective to investigate which concepts shape citizens’ perceptions of microplastics. A qualitative content analysis was used, with coding categories and subcategories developed inductively. Results showed that interviewees formed assumptions around microplastics despite own uncertainties, transferred knowledge from macro‐ to microplastics, and used the concepts of accumulation and dose–response relationship to make sense of the topic. Moreover, they saw the domains of human health and the environment as intertwined and expressed helplessness when discussing solutions to the microplastics issue. Many themes on the topic were similar in both samples, but there were also some differences. For instance, whereas Italian participants talked about marine‐related microplastics, German participants talked about airborne sources; also, German participants tended to recognize more strongly the actions their country was putting in place to address the problem. These findings underscore the need for proactive risk communication despite remaining gaps in scientific risk assessment. Beyond providing technical information, communicators should consider the reasoning behind risk perception on microplastics and address scientific uncertainty as well as the interconnectedness between the domains of human health and the environment.
Flood insurance literacy and flood risk knowledge: Evidence from Portland, Oregon
Authors: Kousky Carolyn , Netusil Noelwah R. 
Published: June 2023

AbstractFlood costs are escalating due to climate change and increased development in hazardous areas. Flood insurance plays a critical role in financial recovery, but there is very little research on how well consumers understand flood insurance policies. Poor insurance literacy could lead to suboptimal risk management decisions. This paper presents some of the first evidence on homeowner flood insurance literacy from a survey of residents in Portland, Oregon. We find that while a basic understanding of general insurance terms is widespread, details about flood insurance policies are not well‐understood and many respondents do not know the specific terms of their policy. Residents of the 100‐year floodplain are more likely to know that their homeowners’ insurance does not cover flooding, but do not have substantially better understanding or knowledge of specifics about their policy than those outside the floodplain. We also find that the majority of survey respondents did not learn about their flood risk or the cost of flood insurance until after making an offer on their property.
Performance ranking, span of control, and grassroots government responsiveness
Authors: Zhang Youlang , Zhai Wenkang , Li Wenzhao 
Published: June 2023

AbstractPolicy makers have increasingly relied on performance ranking regimes based on information collected through public complaint systems to improve grassroots governments' accountability, transparency, and responsiveness. This article contributes to the existing literature by examining whether grassroots governments strategically adjust their responsiveness according to their ranking positions and the number of their peers that are supervised by the same superior government (i.e., span of control). To test the theorized relationships, we built an original panel dataset consisting of monthly responsiveness information and socioeconomic and leadership characteristics of 333 grassroots governments in Beijing, China from February 2019 to December 2020. Our empirical findings consistently show that having a high/low ranking is associated with a decrease/increase in grassroots governments' responsiveness in the next period. Additionally, as the span of control increases, the effect of ranking position on responsiveness changes among grassroots governments weakens. A series of robustness checks were used to validate the theorized relationships.
Experienced versus decision utility: large‐scale comparison for income–leisure preferences*
Authors: Akay Alpaslan , Bargain Olivier B. , Jara H. Xavier 
Published: June 2023

AbstractSubjective well‐being (SWB) data are increasingly used to perform welfare analysis. Interpreted as “experienced utility”, it has recently been compared to “decision utility” using small‐scale experiments most often based on stated preferences. We transpose this comparison to the framework of non‐experimental and large‐scale data commonly used for policy analysis, focusing on the income–leisure domain where redistributive policies operate. Using the British Household Panel Survey, we suggest a “deviation” measure, which is simply the difference between actual working hours and SWB‐maximizing hours. We show that about three‐quarters of individuals make decisions that are not inconsistent with maximizing their SWB. We discuss the potential channels that explain the lack of optimization when deviations are significantly large. We find proxies for a number of individual and external constraints, and show that constraints alone can explain more than half of the deviations. In our context, deviations partly reflect the inability of the revealed preference approach to account for labor market rigidities, so the actual and SWB‐maximizing hours should be used in a complementary manner. The suggested approach based on our deviation metric could help identify labor market frictions.
Testing the bidirectional relationship between belief in COVID‐19 conspiracy theories and psychological distress: A five‐wave longitudinal study
Authors: Chan Hoi‐Wing , Hong Ying‐yi , Lin Li 
Published: June 2023

AbstractPrevious studies have primarily focused on understanding why people believe conspiracy theories, especially during societal crises (e.g., the COVID‐19 pandemic). The investigation of how such conspiracy beliefs would influence people's mental well‐being has just begun recently. The present research aims to address this crucial question by testing the relationships between psychological distress and COVID‐19 conspiracy beliefs with a five‐wave longitudinal study. On the one hand, COVID‐19 conspiracy beliefs could be more appealing to people with heightened distress, as these theories apparently help people to make sense of the uncertainty and life‐threatening disease outbreak. On the other hand, conspiracy theories could be a source of existential threat and thus, would induce rather than reduce psychological distress. We tested these possibilities empirically by a series of cross‐lagged model analyses. Using the random intercept cross‐lagged panel model analysis, we only found a between‐person association but not a cross‐lagged within‐person relationship between the two. COVID‐19 conspiracy beliefs was predicted by being more politically conservative. These findings were further corroborated by the supplementary latent growth curve analyses. Overall, our findings suggest that conspiracy beliefs may not induce or reduce psychological distress in the context of COVID‐19.
A test of vaccine endorsement by political in‐ versus out‐group sources: Effect on vaccination likelihood and exploration of mediation through perceived bias and liking
Authors: Wallace Laura E. , Wegener Duane T. 
Published: June 2023

AbstractThis study investigated whether political endorsements from in‐ versus out‐group political elites would influence likelihood of COVID‐19 vaccination. In March 2021, we ran an experiment with Democrats and Republicans in the United States to examine whether they would be more likely to get vaccinated following endorsements by former Presidents Obama or Trump. Participants reported greater likelihood of getting vaccinated if the vaccine was endorsed by an elite from their own rather than the opposing party. This effect was driven by Trump, who increased vaccination likelihood among Republicans but decreased it among Democrats. We also investigated the mechanisms underlying this persuasion effect and found that perceived bias and liking were plausible mediators, whereas perceived trustworthiness and expertise were not. This study highlights the potential of having endorsements from both Democrat and Republican political elites to increase support for health behaviors in a politically charged climate.