bluuebunny/crossref_metadata_2025_split huggingface dataset:
Poultry cruor hydrolysate is a new potential source of hemoglobin: obtaining of active peptides
Authors: Zouari Oumaima , Deracinois Barbara , Flahaut Christophe , Przybylski Rémi , Nedjar Naima 
Published: June 2023

Abstract
The hydrolysates of animal proteins from Agri-Resources have been the subject of numerous studies for their potential which makes it possible to produce molecules with high added value thanks to their richness in bioactive peptides obtained after enzymatic hydrolysis. The poultry cruor represents an important co-product from slaughterhouses. The aim of this study is to characterize this new source of peptides to be valorized as feed additives. In this work, the conditions of peptides production were fist studied, and revealed that hydrolysis and discoloration are both optimal at pH 3, and that the optimal initial substrate concentration is at 9% (w/v). The potential of poultry cruor to contain bioactive peptides was then studied in silico by comparing poultry with bovine hemoglobin derived peptides using bioinformatic tools. The blast results showed the presence of high similarities between poultry hemoglobin and bovine hemoglobin sequences with identities of 71.13% and 64.34% for α and β chains respectively. The prediction of cleavage sites of poultry hemoglobin was also carried out using peptidecutter software and compared to bovine hemoglobin peptides. The results the presence of similar peptides of poultry cruor hydrolysates comparing to bovine hemoglobin hydrolysates with generation of many new peptides. Mass spectrometry analysis was carried out to determine of bioactive peptides in poultry cruor hydrolysate based on those defined in previous studies. Results revealed the presence of 28 bioactive peptides with mainly opioid and antibacterial peptides. The antibacterial activity was then inspected in vitro against 6 different strains. Results revealed bacterial growth inhibition with interesting MIC values (10 mg/mL against M.luteus E.coli and S.aureus,1.25 mg/mL against K.rhizophilia and 20 mg/mL against S.entirica and L.innocua). The antioxidant activity was also evaluated using different tests. The β-carotene bleaching inhibition activity revealed a RAA of 79.23 ± 1.4%. The DPPH•+ trapping assay an antiradical activity of poultry cruor hydrolysate of 829.35 ± 21.12 µmol/mL and 708.85 ± 0.66 µmol/mL at 40 mg/mL and 20 mg/mL of hydrolysate respectively which is greater than BHT at 0.1 mg/mL and neokyotorphin. The ABTS radical scavenging method revealed inhibition percentages are higher than 90% for hydrolysate concentration above 10 mg/mL which are higher than those obtained with BHT at 0.5 mg/mL. and Finally, the Total antioxidant capacity (TAC) assay showed that the studied hydrolysate have a TAC comprised between that of BHT at 0.3 mg/mL and 0.1 mg/mL. Consequently, these important biological activities found in poultry cruor hydrolysate make it a new interesting alternative natural additive in food industry.
Characterization and Evaluation of Agro-industrial Residues of Bacuri (Platonia Insignis Mart.) and Sweet Cassava (Manihot Esculenta Crantz) in the Production of 6pentyl-α-pyrone by Solid State Fermentation
Authors: NASCIMENTO ADRIANA SILVA DO 
Published: June 2023

Abstract
SUMMARY

The 6 pentyl-α-pyrone (6-PP) is a natural aroma, with a coconut smell, obtained by fermentation processes with fungi of the genus Trichoderma. The objective of this research was to evaluate the nutritional potential of bacuri bark and sweet cassava peels in the production of coconut bioaroma through solid state fermentation (SSF). To this end, analyses of physicochemical characterization, antimicrobial, volatile, surface, and composition activities were performed, including lignocellulosic profile, among others. The fermentations were conducted under humidification conditions with water, nutrient solution without additive and nutrient solutions with glucose or sucrose during 9 days. The quantification of aroma compounds was performed by gas chromatography, aided by solid phase microextraction. The results showed that there was inhibition of fungal growth in the bacuri residue motivated by the presence of inhibitory compounds, especially resin. The residues of sweet cassava, with a high percentage of starch, were favorable to microbial growth, however, the quantification of the bioaroma presented values below 7.0 ppm. Therefore, strategies to increase this production were necessary and the use of sweet cassava bark as a solid supplement, mixed with bacuri residue, was satisfactory reaching concentrations of 26.43±5.72ppm in the presence of nutrient solution with glucose; 42± 6.8ppm with nutrient solution only; 142±2.46 ppm only with distilled water and 202.46±1.30 ppm with sucrose nutrient solution, showing the efficiency of the mixture for fungal development conditions and also for aroma production.
MicroRNAs in Parkinson’s disease: A systematic review and diagnostic accuracy meta-analysis
Authors: Guévremont Diane , Roy Joyeeta , Cutfield Nicholas , Williams Joanna 
Published: June 2023

Abstract
Current clinical tests for Parkinson’s disease (PD) provide insufficient diagnostic accuracy leading to an urgent need for improved diagnostic biomarkers. MicroRNAs (miRNAs) are promising biomarkers of various diseases, including PD. This systematic review and meta-analysis aimed to pool all available data and assess the diagnostic accuracy of biofluid miRNAs in PD. All studies reporting data on miRNAs and their directions of change in PD patients compared to controls were included. Gene targets and significant pathways associated with miRNAs expressed in more than 3 biofluid studies with the same direction of change were analyzed using target prediction and enrichment analysis. A bivariate model was used to calculate sensitivity, specificity, positive and negative likelihood ratios, and diagnostic odds ratio. While miR-24-3p and miR-214-3p were the most reported miRNA in different studies (7 each), miR-331-5p was found to be consistently up regulated in 4 different sources of biofluid. Importantly, miR-19b-3p, miR-24-3p, miR-146a-5p, and miR-221-3p were identified to be reported without conflicting directions of change in the same biofluid type (serum) in multiple studies and bioinformatic analysis found the targets of these miRNAs to be associated with pathways important in PD pathology. Of the 102 studies from the systematic review, 17 were meta-analyzed. Meta-analysis showed that biofluid miRNAs can discriminate between PD patients and controls with good sensitivity (0.82, 95% CI 0.76–0.87) and specificity (0.80, 95% CI 0.74–0.84) with an AUC of 0.87 (95% CI 0.83–0.89). But we found multiple studies failed to match the sex ratios in their groups therefore possibly introducing a selection bias where overall more males with PD were studied than any other group. Overall, our study captures key miRNAs which may represent a point of focus for future studies and the development of diagnostic panels whilst also highlighting the importance of appropriate study design to develop representative biomarker panels for the diagnosis of PD.
Association between gut microbiota and male infertility: a two-sample Mendelian randomization study
Authors: Deng Runpei , Huang Yebao , Tian Zhaohui , Zeng Qingqi 
Published: June 2023

Abstract
Purpose:
Previous research has confirmed the significant association between gut microbiota (GM) and male infertility (MI), but the causality between them remains unclear. This study aims to investigate the causal relationship between GM and MI using Mendelian randomization (MR) and provide supplementary information for the optimization of future randomized controlled trials (RCTs).
Materials and Methods:
Instrumental variables for 211 GM taxa were obtained from genome-wide association studies (GWAS), and inverse variance weighted (IVW) method was used as the main analysis method for two-sample MR analysis to assess the impact of GM on the risk of MI using FinnGen GWAS data (1,128 cases and 110,070 controls). Four methods were used to test for horizontal pleiotropy and heterogeneity of MR results to ensure the reliability of the MR findings.
Results:
A total of 50 single nucleotide polymorphisms (SNPs) closely related to GM were included, and we found that family-Bacteroidaceae was significantly associated with MI (odds ratio [OR] = 0.54, 95% confidence interval [CI]: 0.31–0.96, P = 0.035). Furthermore, we used four MR methods to evaluate the causality, and the results supported these findings. The leave-one-out analysis showed stable results with no instrumental variables exerting strong influence on the results. The causal direction indicated a positive effect, and the effects of heterogeneity and horizontal pleiotropy on the estimation of causal effect were minimized.
Conclusions:
We confirmed a causal relationship between GM taxa and MI, providing new insights into the mechanisms underlying GM-mediated MI. Further comprehensive and larger sample studies are needed to explore the relationship between GM and MI for identification of novel biomarkers and targeted prevention strategies for MI in future research.
Identification effective diagnosis biomarkers and immune cell infiltration in liver cancer by bioinformatics analysis and machine learning algorithm
Authors: ge shuxiong , wang hui 
Published: June 2023

Abstract
Objective
The aim of this study was to identify the key diagnosis biomarkers of abnormal expression and immune infiltration in liver cancer based on bioinformatics analysis and machine learning algorithm.
Methods
Three microarray datasets were obtained from Gene Expression Omnibus database, of which GSE88389 and GSE121248 were defined as training sets and GSE45436 was defined as a validation set. Differentially expressed genes (DEGs) were identified and functional and pathway enrichment analysis was performed by Gene Ontology terms (GO), Kyoto Encyclopedia of Genes (KEGG), disease enrichment analysis (DO) and gene enrichment analysis (GSEA). Tumor biomarkers for liver cancer were identified through Lasso and support vector machine (SVM) and validated in the GSE45436. CIBERSORT was performed to analyze the relation between the diagnosis biomarkers for liver cancer and immune cell infiltration.
Results
A total of 39 differentially expressed genes (DEGs), including 6 up-regulated and 33 down-regulated genes, were obtained based on expression fold change and significance. Analysis of GO, KEGG, DO and GSEA pathways indicated that these DEGs were enriched in collagen-containing extracellular matrix, collagen trimer, plasma lipoprotein particle, bile secretion, tryptophan metabolism, retinol metabolism, chemical carcinogenesis - DNA adducts, C-type lectin receptor signaling pathway, acute porphyria, lung squamous cell carcinoma, cell cycle, DNA replication, proteasome and ribosome. Combination analysis of Lasso and Support vector machine (SVM), three diagnosis value genes of CAP2、CXCL14 and TMEM27 for liver cancer were obtained by Venn diagram. Compared with normal tissue, immune infiltration analysis demonstrated that naive B cells, memory B cells, regulatory T cells and macrophages were highly expressed in hepatocellular carcinoma tissues, while plasma cells and T cells were low expression.
Conclusion
We identified CAP2、CXCL14 and TMEM27 as potential biomarkers for liver cancer and that can mediate immune cell activity in liver cancer.
A Mendelian randomization-based study of the causal relationship between cardiovascular-related diseases and endocarditis
Authors: Li Jianhua , Lv Xin , Meng Lingwei , Liu Chuanzhen 
Published: June 2023

Abstract
Objective: To investigate the causal relationship between cardiovascular-related diseases (coronary artery disease, heart failure, diabetes mellitus, systolic and diastolic blood pressure) and endocarditis.
Methods: A two-sample Mendelian randomization (MR) study was performed to evaluate the causal effect of cardiovascular-related diseases on endocarditis. Statistical summaries for the associations between single nucleotide polymorphisms (SNPs) and traits of interest were obtained from independent consortia with European populations. The dataset of cardiovascular-related diseases was acquired from genome-wide association studies (GWAS), including cases and controls (coronary artery disease:122733 cases and 424528 controls, hear failure: 47309 cases and 930014 controls, diabetes mellitus: 61714 cases and 1178 controls, systolic and diastolic blood pressure: 757601 samples). A GWAS with 1080 endocarditis cases and 485404 controls was used to identify genetic variation underlying cardiovascular-related diseases. Five complementary MR methods were performed, including inverse variance weighted method (IVW), MR-Egger, weighted median, weighted mode, simple mode.
Results: Genetically predicted coronary artery disease (odds ratio [OR] 1.326, 95%CI 1.085-1.622), heart failure (OR 1.961, 95%CI 1.103-3.487), systolic blood pressure (OR 1.039, 95%CI 1.023-1.055) and diastolic blood pressure (OR 1.030, 95%CI 1.002-1.059) was significantly positively associated with endocarditis. Diabetes was not associated with endocarditis (p=0.33).
Conclusion: Coronary arterial disease, heart failure and hypertension were high risk factors of endocarditis while diabetes was not.
Observation of acoustic spatiotemporal vortices
Authors: Zhang Hongliang , Sun Yeyang , Huang Junyi , Wu Bingjun , Yang Zhaoju , Bliokh Konstantin , Ruan Zhichao 
Published: June 2023

Abstract
Vortices in fluids and gases have piqued the interest of human for centuries. Development of classical-wave physics and quantum mechanics highlighted wave vortices characterized by phase singularities and topological charges. In particular, vortex beams have found numerous applications in modern optics and other areas. Recently, optical spatiotemporal vortex states exhibiting the phase singularity both in space and time have been reported. Here, we report the first generation of acoustic spatiotemporal vortex pulses. We utilize an acoustic meta-grating with mirror-symmetry breaking as the spatiotemporal vortex generator. In the momentum−frequency domain, we unravel that the transmission spectrum functions exhibit a topological phase transition where the vortices with opposite topological charges are created or annihilated in pairs. Furthermore, with the topological textures of the nodal lines, these vortices are robust and exploited to generate spatiotemporal vortex pulse against structural perturbations and disorder. Our work paves the way for studies and applications of spatiotemporal structured waves in acoustics and other wave systems.
Elevated CO2 improves the growth of grapevine young plants under salinity favoring ionic homeostasis and decreasing oxidative damages
Authors: Hermínio Pedro José , Santos Hugo Rafael Bentzen , Lima Rachel Hellen Vieira Sousa , Silveira Joaquim Albenísio Gomes , Silva Thieres George Freire da , Neto Juracy Barroso , Simões Adriano Nascimento , Souza Eduardo , Ferreira-Silva Sérgio Luiz 
Published: June 2023

Abstract
This study tested the hypothesis that elevated CO2 improves the growth of grapevine seedlings, achieving greater vigor and shortening the production time under salinity due to the best ionic homeostasis and oxidative protection. Seedlings were exposed to isolate and combined effects of CO2 (ambient – 400 and elevated – 800 µmol mol− 1) and salinity (absence – 0 and presence – 80 mM NaCl) in a growth chamber. In these conditions, two-old-month grapevine (Vitis ssp.) plants were grown and defoliated after 20 days, followed by more than 60 days of growth to the emission of new shoots (buds, leaves, and stems). Salinity delays and decreases the emission of new shoots, while elevated CO2 improves the growth of new shoots. Elevated CO2 promoted the leaf dry matter (DM) contends and shoot/root ratio in the absence and presence of salt, and the partial recovery of losses leaf DM under salt. High CO2 mitigated ionic toxicity under salt stress through lower leaf Cl− and Na+ accumulation with a preferential allocation of these ions in stems and roots, relative to plants under salt alone. Elevated CO2 stimulated the K+ content in the whole plant (leaves/stems/roots), in salt absence and presence, with increases for K+/Na+ ratios in leaves and roots under salinity. High CO2 favored the water potential (ΨW) reduction under salinity and increase water relative content (RWC) of plants under two salt levels. Under salt absence, photochemical efficiency indicators quenching photochemical (qP), electron transport rate (ETR), and non-photochemical quenching (NPQ) showed that photosynthesis was significantly favored by high CO2 and the effect was also important for mitigating the salt impact for CO2 uptake. Taken together, data show that elevated CO2 has the potential to improve grapevine seedlings’ production system, mainly under salt stress.
Leucas Aspera leaves extract as a copper corrosion inhibitor in 0.5M H2 SO4 medium
Authors: sankar S.Jyothi , narayanaperumal N.Kavitha 
Published: June 2023

AbstractThe corrosion inhibitive action of leaves extracts ofLeucas Asperaon copper corrosion in 0.5M H2SO4solution was studied using the weight-loss method, Potentiodynamic polarization, EIS measurements, SEM-EDXS and FT-IR spectroscopic techniques. The results obtained indicate that the extracts functioned as good inhibitors in 0.5M H2SO4solution. The inhibition efficiency was found to increase with extract concentration. Potentiodynamic polarization curves indicated that the plant extracts behave as mixed-type inhibitors. The thermodynamic parameters showed that the adsorption of the extracts on copper is physical, spontaneous, and favoured at low temperatures. The data for the adsorption of extract fit well with Langmuir adsorption isotherm. FTIR results showed that the inhibition mechanism was by the absorption process, through the functional groups present in the extract. The SEM images of the corrosion product confirmed the protection offered by the extract on the surface of the metal immersed in the 0.5M H2SO4medium.
Promoting Intercultural Competence Among International Kindergarten Teachers in a Glocalization Context: Exploring the Impact of Gather.town-Integrated HyFlex Learning Model on Intercultural Mindfulness and Empathy
Authors: Chen Guanzheng 
Published: June 2023

Abstract
Background: With the glocalization context and increased cultural diversity in international kindergartens in China, this mixed-methods study aimed to investigate the efficacy of innovative learning models, particularly the HyFlex learning model and Gather.town platform, in fostering intercultural mindfulness and empathy among international kindergarten teachers.
Methods: Employing a pre-test-post-test control group design, 80 participants from four international kindergartens were randomly allocated to experimental (n=40) and control (n=40) groups. Alongside quantitative methods, qualitative data were collected from observational field notes and semi-structured interviews to provide in-depth insights into participants' experiences and intercultural competence development.
Results: Quantitative findings revealed that the experimental group consistently exhibited higher levels of intercultural mindfulness and empathy post-intervention compared to the control group. The qualitative analysis further suggested that the experimental group reported increased awareness of biases, enhanced empathy, heightened engagement, and more effective application of intercultural skills.
Conclusions: The study contributes to intercultural competence literature by providing empirical evidence supporting the effectiveness of the HyFlex learning model and Gather.town platform in fostering intercultural mindfulness and empathy among international kindergarten teachers in a glocalization context. The findings suggest significant implications for refining teacher education and professional development programs through the integration of innovative learning approaches. However, future research should address the study's limitations, including the small sample size, the exclusive focus on international kindergarten teachers, and the reliance on self-report measures.
Hydrologic Modelling for Flood Threshold and Hazard Prediction in the Black Volta River Basin, West Africa
Authors: Yeboah Felicia , ACKOM EDWARD KOFI , Yidana Sandow Mark , Awotwi Alfred 
Published: June 2023

Abstract
The Black Volta River Basin (BVRB) is one of the flood-prone subbasins in the West African subregion experiencing frequent and severe flood-related disasters in recent times. Flood prediction systems embedded with hydrological models are important non-structural measures for effective flood management. This study adopted a Hydrological Modeling approach using Hydrological Engineering Center’s Hydrological Modeling System (HEC-HMS) software to simulate flood events within the BVRB. The future average frequency of occurrence of flood events in terms of peak flows was simulated using the calibrated and validated model and Intensity-Duration-Frequency (IDF) curves for the basin. This was followed by the application of a rating curve to obtain floodwater levels corresponding to the predicted peak discharges and subsequently, a flood hazard classification ranging from moderate, high, and very high severity levels in terms of peak flow and water levels.
The Nash–Sutcliffe model efficiency (NSE) coefficient results of > 0.70 for both calibration and validation indicate the model’s good performance in the basin. The model application for flood threshold prediction and hazard classification in terms of peak flows and flood water levels in the basin particularly at the Chache station reveals that a severe flood of magnitude (flood water level >= 8 m and equivalent discharge >= 1935 m3/s) is likely to be experienced in the basin for a rainfall event of at least 25-year return period. Moderate flooding is likely to be experienced for at least a 5-year return period rainfall event (7.0 m =< flood water level < 7.5 m and 1365 m3/s =< peak flow < 1635 m3/s) in the study area. The outcomes of this study help to better understand the hydrological response and flood characterization due to extreme rainfall events for flood monitoring purposes in the BVRB.
Grouping rule in tadpole: is the numerical quantity or size-assortative more critical?
Authors: Hase Kazuko 
Published: June 2023

Abstract

The ability to perceive group size and discriminate the ontogeny of conspecifics would play a crucial role in the grouping behavior of animals. However, the relative importance of numerical quantity and size-assortative preferences in shaping grouping rules remains poorly understood. In this study, I examined the responses of Miyako toad (Bufo gargarizans miyakonis) tadpoles to number quantity and size discrimination by choice tests at different ontogenetic stages (small, medium, and large). The results revealed that small-sized tadpoles in early developmental stages significantly preferred larger numbers (4) compared to smaller ones (1). However, this preference was not observed in later developmental stages (medium and large). And interestingly, when there was no group size bias, size discrimination was not observed in tadpoles, irrespective of their ontogeny. These findings suggest that Miyako toad tadpoles possess innate numerical competence but demonstrate ontogeny-dependent utilization of this ability. Understanding the interplay between numerical quantity and size-assortative preferences in grouping behavior will provide esteemed insights into the adaptive value of number sense in vertebrates and sheds light on evolutionary processes.