bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma phosphorylated tau181 as a biomarker of mild traumatic brain injury: findings from THINC and NCAA-DoD CARE Consortium prospective cohorts
Authors: Devoto Christina , Vorn Rany , Mithani Sara , Meier Timothy B. , Lai Chen , Broglio Steven P. , McAllister Thomas , Giza Christopher C. , Huber Daniel , Harezlak Jaroslaw , Cameron Kenneth L. , McGinty Gerald , Jackson Jonathan , Guskiewicz Kevin , Mihalik Jason P. , Brooks Alison , Duma Stefan , Rowson Steven , Nelson Lindsay D. , Pasquina Paul , Turtzo Christine , Latour Lawrence , McCrea Michael A. , Gill Jessica M. 
Published: August 2023

ObjectiveThe aim of this study was to investigate phosphorylated tau (p-tau181) protein in plasma in a cohort of mild traumatic brain injury (mTBI) patients and a cohort of concussed athletes.MethodsThis pilot study comprised two independent cohorts. The first cohort—part of a Traumatic Head Injury Neuroimaging Classification (THINC) study—with a mean age of 46 years was composed of uninjured controls (UIC, n = 30) and mTBI patients (n = 288) recruited from the emergency department with clinical computed tomography (CT) and research magnetic resonance imaging (MRI) findings. The second cohort—with a mean age of 19 years—comprised 133 collegiate athletes with (n = 112) and without (n = 21) concussions. The participants enrolled in the second cohort were a part of a multicenter, prospective, case-control study conducted by the NCAA-DoD Concussion Assessment, Research and Education (CARE) Consortium at six CARE Advanced Research Core (ARC) sites between 2015 and 2019. Blood was collected within 48 h of injury for both cohorts. Plasma concentration (pg/ml) of p-tau181 was measured using the Single Molecule Array ultrasensitive assay.ResultsConcentrations of plasma p-tau181 in both cohorts were significantly elevated compared to controls within 48 h of injury, with the highest concentrations of p-tau181 within 18 h of injury, with an area under the curve (AUC) of 0.690–0.748, respectively, in distinguishing mTBI patients and concussed athletes from controls. Among the mTBI patients, the levels of plasma p-tau181 were significantly higher in patients with positive neuroimaging (either CT+/MRI+, n = 74 or CT−/MRI+, n = 89) compared to mTBI patients with negative neuroimaging (CT−/MRI−, n = 111) findings and UIC (P-values &lt; 0.05).ConclusionThese findings indicate that plasma p-tau181 concentrations likely relate to brain injury, with the highest levels in patients with neuroimaging evidence of injury. Future research is needed to replicate and validate this protein assay's performance as a possible early diagnostic biomarker for mTBI/concussions.
Case report: Off-label use of low-dose perampanel in a 25-month-old girl with a pathogenic SYNGAP1 variant
Authors: Gupta Siddharth , Hwang Yun , Ludwig Natasha , Henry Julia , Kadam Shilpa D. 
Published: August 2023

IntroductionPreclinical studies in a mouse model have shown that SYNGAP1 haploinsufficiency results in an epilepsy phenotype with excessive GluA2-AMPA insertion specifically on the soma of fast-spiking parvalbumin-positive interneurons associated with significant dysfunction of cortical gamma homeostasis that was rescued by perampanel (PER), an AMPA receptor blocker. In this single case, we aimed to investigate the presence of dysregulated cortical gamma in a toddler with a pathogenic SYNGAP1 variant and report on the effect of low-dose PER on electroencephalogram (EEG) and clinical profile.MethodsClinical data from physician's clinic notes; genetic testing reports; developmental scores from occupational therapy, physical therapy, speech and language therapy evaluations; and applied behavioral analysis reports were reviewed. Developmental assessments and EEG analysis were done pre- and post-PER.ResultsClinically, the patient showed improvements in the developmental profile and sleep quality post-PER. EEG spectral power analysis in our patient revealed a loss of gamma power modulation with behavioral-state transitions similar to what was observed in Syngap1+/− mice. Furthermore, the administration of low-dose PER rescued the dysfunctional cortical gamma homeostasis, similar to the preclinical study. However, as in the epileptic mice, PER did not curb epileptiform discharges or clinical seizures.ConclusionSimilar to the Syngap1+/− mice, cortical gamma homeostasis was dysregulated in the patient. This dysfunction was rescued by PER. These encouraging results necessitate further validation of gamma dysregulation as a potential translational EEG biomarker in SYNAP1-DEE. Low-dose PER can be explored as a therapeutic option through clinical trials.
Effectiveness of chemotherapy using bortezomib combined with homoharringtonine and cytarabine in refractory or relapsed acute myeloid leukemia: a phase II, multicenter, prospective clinical trial
Authors: Zhang Chengtao , Gao Da , Wang Xiaohong , Sun Xiuli , Yan Yan , Yang Yan , Zhang Jingjing , Yan Jinsong 
Published: August 2023

BackgroundRefractory/relapsed acute myeloid leukemia (R/R AML) has unsatisfactory outcomes even after allogeneic hematopoietic stem cell transplantation. Long-term survival is mainly influenced by complete remission (CR) rates after induction therapies.ObjectivesTo investigate CR/CR with incomplete hematologic recovery (CRi) rates and adverse events with a new induction therapy (bortezomib, homoharringtonine, and cytarabine [BHA]) for patients with R/R AML.MethodsWe enrolled 21 patients with R/R AML (median age, 42 [range, 30–62] years), who received BHA for remission induction (bortezomib, 1.3 mg/m2/day on days 1 and 4; homoharringtonine, 4 mg/m2/day for 5 days, and cytarabine, 1.5 g/m2/day for 5 days). CR and adverse events were assessed.ResultsAfter one course of BHA, the CR/CRi and partial remission rates were 38.1% and 14.3%, respectively, with an overall response rate (ORR) of 52.4% in 21 patients. 9 of 21 patients harbored FLT3-ITD or FLT3-TKD mutations, and achieved either CR/CRi or ORR of 66.7% (P=0.03) by comparison with that in R/R AML without FLT3 mutation. After induction therapy, consolidation chemotherapy or allogeneic hematopoietic stem cell transplantation led to a one-year overall survival of 27.8% in all patients. One-year relapse-free survival was 50% in 8 patients who had achieved CR/CRi after one course of BHA. During induction, non-hematologic adverse events (grade 3/4) commonly were infection (90.5%), hypokalemia (14.4%), hypocalcemia (14.3%), and mucositis (9.5%). In patients achieving CR, the median time to neutrophil count &gt;0.5×109/L and time to platelet count &gt;20×109/L were 15 (13–17) days and 13 (13–18) days, respectively.ConclusionBHA chemotherapy regimen was safe and tolerable to serve as an induction therapy for R/R AML, particularly with FLT3 mutation. The higher CR/CRi rate will give a clue to determine a potentialeffectiveness of BHA for AML patients carrying FLT3 mutation in a further investigation.Clinical trial registrationhttps://www.chictr.org.cn/, identifier ChiCTR2000029841.
An anoikis-related gene signature for prediction of the prognosis in prostate cancer
Authors: Zhao Xiaodong , Wang Zuheng , Tang Zilu , Hu Jun , Zhou Yulin , Ge Jingping , Dong Jie , Xu Song 
Published: August 2023

PurposeThis study presents a novel approach to predict postoperative biochemical recurrence (BCR) in prostate cancer (PCa) patients which involves constructing a signature based on anoikis-related genes (ARGs).MethodsIn this study, we utilised data from TCGA-PARD and GEO databases to identify specific ARGs in prostate cancer. We established a signature of these ARGs using Cox regression analysis and evaluated their clinical predictive efficacy and immune-related status through various methods such as Kaplan-Meier survival analysis, subject work characteristics analysis, and CIBERSORT method. Our findings suggest that these ARGs may have potential as biomarkers for prostate cancer prognosis and treatment. To investigate the biological pathways of genes associated with anoikis, we utilised GSVA, GO, and KEGG. The expression of ARGs was confirmed by the HPA database. Furthermore, we conducted PPI analysis to identify the core network of ARGs in PCa.ResultsBased on analysis of the TCGA database, a set of eight ARGs were identified as prognostic signature genes for prostate cancer. The reliability and validity of this signature were well verified in both the TCGA and GEO codifications. Using this signature, patients were classified into two groups based on their risk for developing BCR. There was a significant difference in BCR-free time between the high and low risk groups (P &lt; 0.05).This signature serves as a dependable and unbiased prognostic factor for predicting biochemical recurrence (BCR) in prostate cancer (PCa) patients. It outperforms clinicopathological characteristics in terms of accuracy and reliability. PLK1 may play a potential regulatory role as a core gene in the development of prostate cancer.ConclusionThis signature suggests the potential role of ARGs in the development and progression of PCa and can effectively predict the risk of BCR in PCa patients after surgery. It also provides a basis for further research into the mechanism of ARGs in PCa and for the clinical management of patients with PCa.
Fabricating niosomal-PEG nanoparticles co-loaded with metformin and silibinin for effective treatment of human lung cancer cells
Authors: Salmani-Javan Elnaz , Jafari-Gharabaghlou Davoud , Bonabi Esat , Zarghami Nosratollah 
Published: August 2023

BackgroundDespite current therapies, lung cancer remains a global issue and requires the creation of novel treatment methods. Recent research has shown that biguanides such as metformin (MET) and silibinin (SIL) have a potential anticancer effect. As a consequence, the effectiveness of MET and SIL in combination against lung cancer cells was investigated in this study to develop an effective and novel treatment method.MethodsNiosomal nanoparticles were synthesized via the thin-film hydration method, and field emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR), atomic force microscopy (AFM), and dynamic light scattering (DLS) techniques were used to evaluate their physico-chemical characteristics. The cytotoxic effects of free and drug-loaded nanoparticles (NPs), as well as their combination, on A549 cells were assessed using the MTT assay. An apoptosis test was used while under the influence of medication to identify the molecular mechanisms behind programmed cell death. With the use of a cell cycle test, it was determined whether pharmaceutical effects caused the cell cycle to stop progressing. Additionally, the qRT-PCR technique was used to evaluate the levels of hTERT, BAX, and BCL-2 gene expression after 48-h medication treatment.ResultsIn the cytotoxicity assay, the growth of A549 lung cancer cells was inhibited by both MET and SIL. Compared to the individual therapies, the combination of MET and SIL dramatically and synergistically decreased the IC50 values of MET and SIL in lung cancer cells. Furthermore, the combination of MET and SIL produced lower IC50 values and a better anti-proliferative effect on A549 lung cancer cells. Real-time PCR results showed that the expression levels of hTERT and BCL-2 were significantly reduced in lung cancer cell lines treated with MET and SIL compared to single treatments (p&lt; 0.001).ConclusionIt is anticipated that the use of nano-niosomal-formed MET and SIL would improve lung cancer treatment outcomes and improve the therapeutic efficiency of lung cancer cells.
Clinical and radiological characteristics of pediatric COVID-19 before and after the Omicron outbreak: a multi-center study
Authors: Lin Bin , Xu Xiaopei , Shen Zhujing , Huang Peiyu , Gao Yuantong , Liu Jun , Xie Zongyu , Zhao Tongtong , Xia Junli , Lv Jian , Ren Dawei , Zheng Hanpeng , Wang Xiangming , Hu Minghua , Ruan Guixiang , Zhang Minming 
Published: August 2023

IntroductionThe emergence of the Omicron variant has seen changes in the clinical and radiological presentations of COVID-19 in pediatric patients. We sought to compare these features between patients infected in the early phase of the pandemic and those during the Omicron outbreak.MethodsA retrospective study was conducted on 68 pediatric COVID-19 patients, of which 31 were infected with the original SARS-CoV-2 strain (original group) and 37 with the Omicron variant (Omicron group). Clinical symptoms and chest CT scans were examined to assess clinical characteristics, and the extent and severity of lung involvement.ResultsPediatric COVID-19 patients predominantly had normal or mild chest CT findings. The Omicron group demonstrated a significantly reduced CT severity score than the original group. Ground-glass opacities were the prevalent radiological findings in both sets. The Omicron group presented with fewer symptoms, had milder clinical manifestations, and recovered faster than the original group.DiscussionThe clinical and radiological characteristics of pediatric COVID-19 patients have evolved with the advent of the Omicron variant. For children displaying severe symptoms warranting CT examinations, it is crucial to weigh the implications of ionizing radiation and employ customized scanning protocols and protective measures. This research offers insights into the shifting disease spectrum, aiding in the effective diagnosis and treatment of pediatric COVID-19 patients.
Effects of blood flow restriction on mechanical properties of the rectus femoris muscle at rest
Authors: Jarosz Jakub , Gaweł Dawid , Krzysztofik Michal , Zając Adam , Tsoukos Athanasios , Bogdanis Gregory C. , Wilk Michal 
Published: August 2023

Introduction: This study examined the effects of blood flow restriction (BFR) and reperfusion on the mechanical properties of the rectus femoris muscle at rest (frequency and stiffness).Methods: Fourteen trained men (body weight = 81.0 ± 10.3 kg; BMI = 25 ± 3.0 m/kg2; height = 181 ± 4 cm; training experience = 6.0 ± 2.2 years) participated in an experimental session involving their dominant (BFR) and non-dominant leg (control). Muscle mechanical properties were measured using Myoton’s accelerometer at the midpoint of the rectus femoris muscle at five time points. In the BFR leg, an 80% arterial occlusion pressure was applied by a cuff for 5 min. No cuff was applied in the control leg. Femoral Myoton measurements were taken from both legs 2 and 4 min after the start of BRF as well as 30 s and 2 min after the end of the occlusion period.Results: The two-way ANOVA revealed a statistically significant interaction effect for stiffness and frequency (p &lt; 0.001; η2 &gt; 0.67). The post hoc analysis showed that both stiffness and frequency increased during BFR compared with rest and then dropped to the resting levels post BFR period. Also, stiffness and frequency were higher than control only during the BFR period, and similar during rest and post BFR.Conclusion: These results indicate that the application of BFR at rest leads to significant changes in mechanical properties of the rectus femoris muscle.
Rapid appearance quality of rice based on machine vision and convolutional neural network research on automatic detection system
Authors: He Yangfan , Fan Baojiang , Sun Lei , Fan Xiaofei , Zhang Jun , Li Yuchao , Suo Xuesong 
Published: August 2023

IntroductionIn the process of rice production and storage, there are many defects in the traditional detection methods of rice appearance quality, but using modern high-precision instruments to detect the appearance quality of rice has gradually developed into a new research trend at home and abroad with the development of agricultural artificial intelligence.MethodsIn this study, we independently designed a fast automatic rice appearance quality detection system based on machine vision technology by introducing convolutional neural network and image processing technology. In this study, NIR and RGB images were generated into five-channel image data by superposition function, and image are preprocessed by combining the Watershed algorithm with the Otus adaptive threshold function. Different grains in the samples were labeled and put in the convolutional neural network for training. The rice grains were classified and the phenotype data were analyzed by selecting the optimal training model to realize the detection of rice appearance quality.Results and discussionThe experimental results showed that the resolution of the system could reach 92.3%. In the detection process, the system designed with this method not only reduces the subjectivity problems caused by different detection environments, visual fatigue caused large sample size and the inspector’s personal factors, but also significantly improves the detection time and accuracy, which further enhances the detection efficiency of rice appearance quality, and has positive significance for the development of the rice industry.
Zinc oxide nanoparticles enhanced rice yield, quality, and zinc content of edible grain fraction synergistically
Authors: Mi Kailiang , Yuan Xijun , Wang Qianyue , Dun Canping , Wang Rui , Yang Shuo , Yang Yanju , Zhang Hongcheng , Zhang Haipeng 
Published: August 2023

Zinc oxide nanoparticles (ZnO NPs) have been widely used in agriculture as a new type of Zn fertilizer, and many studies were conducted to evaluate the effect of ZnO NPs on plant growth. However, there are relatively few studies on the effects of application methods and appropriate dosages of ZnO NPs on rice yield, quality, grain Zn content, and distribution. Therefore, in the 2019 and 2020, field trials were conducted with six ZnO NPs basal application dosages of no ZnO NPs, 3.75 kg hm−2, 7.5 kg hm−2, 15 kg hm−2, 30 kg hm−2, and 60 kg hm−2, and the effects of ZnO NPs application on rice yield, quality, grain Zn content, and distribution were investigated. The results demonstrated that applying ZnO NPs in Zn-deficient soils (available Zn &lt; 1.0 mg kg−1) increased rice grain yield by 3.24%–4.86% and 3.51%–5.12% in 2019 and 2020, respectively. In addition, ZnO NPs improved the quality of rice by increasing the head milling rate, reducing chalky grain percentage, and increasing the taste value and breakdown of rice. In terms of Zn accumulation in rice, ZnO NPs application significantly increased the Zn content in both milled rice and brown rice, compared with no Zn treatment, in 2019 and 2020, Zn content in milled rice significantly increased by 20.46%–41.09% and 18.11%–38.84%, respectively, and in brown rice significantly increased by 25.78%–48.30% and 20.86%–42.00%, respectively. However, the Zn fertilizer utilization gradually decreased with increasing ZnO NPs application dosage. From the perspective of yield, rice quality, Zn fertilizer utilization, and Zn accumulation, basal application of 7.5 kg–30 kg hm−2 ZnO NPs is beneficial for rice yield and quality improvement and rice Zn accumulation. This study effectively demonstrated that ZnO NPs could be a potential high‐performed fertilizer for enhancing rice yield, quality, and zinc content of edible grain fraction synergistically.
Childhood environmental harshness and unpredictability negatively predict eHealth literacy through fast life-history strategy
Authors: Feng Wenfei , Zhang Jinguang 
Published: August 2023

BackgroundeHealth literacy—the ability to obtain, understand, evaluate, and use health information from the Internet—is important to maintaining and improving personal health. Prior research found that people differ notably in the levels of eHealth literacy, and this study tests a theoretical account of some of those individual differences. Drawing on life history theory, we propose that low eHealth literacy is partly the outcome of people adopting a resource-allocation strategy emphasizing early and fast reproduction, namely, a fast life-history strategy.MethodsWe conducted a cross-sectional survey study (N = 1,036) that measured Chinese adult respondents’ eHealth literacy, childhood environmental harshness and unpredictability, and fast life-history strategy. Covariates included health-information seeking online, self-rated health, sex, age, education level, and monthly income.ResultsSupporting a life-history explanation of eHealth literacy, childhood environmental harshness and unpredictability negatively predicted eHealth literacy through fast life-history strategy and mainly the insight-planning-control dimension of it. Harshness, not unpredictability, also directly and negatively predicted eHealth literacy after fast life-history strategy was controlled for.ConclusionOur findings suggest that the psychological mechanisms associated with human life-history strategies produce at least some of the individual differences in levels of eHealth literacy, including those related to neuroticism, socioeconomic status, self-rated health and social capital. Thus, a possible way to increase future generation’s eHealth literacy and thereby their health is to reduce the harshness and unpredictability of the environment in which they grow up, thereby making them more likely to adopt a relatively slow life-history in their adulthood.
Translation, adaptation, and initial evaluation of a guided self-help intervention to reduce psychological distress among nurses during COVID-19 in China
Authors: Tian Tian , Sun Jingwen , Jiang Yue , Guo Qian , Huang Zeyu , Wang Duolao , Rahman Atif , Li Xiaomei , Yang Lei 
Published: August 2023

BackgroundThis study aimed to reduce the unprecedented and intense psychological distress that nurses were forced to experience during the COVID-19 pandemic. A Chinese version of the World Health Organization's Self-Help Plus (SH+) intervention guide was adapted and tested among nurses. The objective of this study was to translate and adapt the SH+ guideline into the Chinese version and to test its feasibility in reducing psychological distress among nurses during COVID-19.MethodsA staged approach comprising translation, adaptations, initial evaluation by pilot implementation, and a qualitative process evaluation was conducted in two hospitals in Xi'an, China. The translation of the Chinese version was authorized by the World Health Organization. We adapted SH+ for use among clinical nurses working during the pandemic in China through a qualitative process evaluation, which was guided by the descriptive phenomenological study design. The outcomes of the pilot included psychological distress, psychological flexibility, depressive and anxiety symptoms, post-traumatic stress disorder (PTSD) symptoms, and subjective psychological wellbeing, which were assessed using the Kessler 6 symptom checklist, the Comprehensive Assessment of ACT Process (CompACT), the Patient Health Questionnaire-9 (PHQ-9), the Generalized Anxiety Disorder scale (GAD-7), the PTSD Checklist-Civilian Version (PCL-C), and the Index of Wellbeing (IWB), respectively.ResultsThe SH+ materials, including audio-recorded sessions and an accompanying illustrated manual, were translated into Chinese and adapted in line with feedback from the nurses. An uncontrolled pilot study (n = 28) for 5 weeks showed a statistically significant reduction of psychological distress (mean difference in Kessler 6 score, −2.74; 95% CI [−3.71, −1.78]; p &lt; 0.001). We also found improvements in psychological flexibility (mean difference in CompACT score, 6.89; 95% CI [−12.35, −4.47]; p &lt; 0.001), subjective psychological wellbeing (mean difference in IWB score, 0.86; 95% CI [0.07, 1.65]; p &lt; 0.05), and depressive symptoms (mean difference in PHQ-9 score, −1.52; 95% CI [−2.78, −0.26]; p &lt; 0.05). The process evaluation showed that nurses found the SH+ program very useful but difficult to adhere to.ConclusionWe found that the translated Chinese version of SH+ was applicable and feasible in the Chinese cultural context. There was a potential effect of adapted SH + in reducing nurses' psychological distress during COVID-19 and suggested the value of exploring strategies to increase adherence to the program.