bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chimeric Antigen Receptor Macrophages Target and Resorb Amyloid Plaques in a Mouse Model of Alzheimer’s Disease
Authors: Pan Qiuyun , Yan Ping , Kim Alexander B. , Xiao Qingli , Pandey Gaurav , Haecker Hans , Epelman Slava , Diwan Abhinav , Lee Jin-Moo , DeSelm Carl J. 
Published: April 2023

AbstractSubstantial evidence suggests a role for immunotherapy in treating Alzheimer’s disease (AD). Several monoclonal antibodies targeting aggregated forms of beta amyloid (Aβ), have been shown to reduce amyloid plaques and in some cases, mitigate cognitive decline in early-stage AD patients. We sought to determine if genetically engineered macrophages could improve the targeting and degradation of amyloid plaques. Chimeric antigen receptor macrophages (CAR-Ms), which show promise as a cancer treatment, are an appealing strategy to enhance target recognition and phagocytosis of amyloid plaques in AD. We genetically engineered macrophages to express a CAR containing the anti-amyloid antibody aducanumab as the external domain and the Fc receptor signaling domain internally. CAR-Ms recognize and degrade Aβin vitroand on APP/PS1 brain slicesex vivo;however, when injected intrahippocampally, these first-generation CAR-Ms have limited persistence and fail to reduce plaque load. We overcame this limitation by creating CAR-Ms that secrete M-CSF and self-maintain without exogenous cytokines. These CAR-Ms have greater survival in the brain niche, and significantly reduce plaque load locallyin vivo. These proof-of-principle studies demonstrate that CAR-Ms, previously only applied to cancer, may be utilized to target and degrade unwanted materials, such as amyloid plaques in the brains of AD mice.Amyloid targeting CAR Macrophages engineered to secrete M-CSF promote their own local survival and expansion while resorbing amyloid plaques in the brains of Alzheimer’s disease APP/PS1 mice, resulting in significant local clearance of amyloid plaques of all sizes.
On the involvement of cell volume regulation mechanisms in post-hypertonic lysis and slow-freezing injury of human erythrocytes and its broader cryobiological significance
Authors: Klbik Ivan 
Published: April 2023

ABSTRACTAn effort is made to explain the salt loading of hypertonicity-treated human red blood cells (RBC) and their post-hypertonic lysis, which occurs upon resuspension to isotonic media. It is recognized that the salt loading is consistent with the regulatory volume increase response - a physiological response applied by cells to adapt to hypertonic conditions. It is therefore hypothesized that salt loading occurs via physiological ion transport pathways, allowing passive dissipative fluxes across the plasma membrane with the preservation of membrane integrity. It is argued that post-hypertonic lysis is essentially a hypotonic lysis occurring already in an isotonic medium due to the excess salt in the cytoplasm. A simplified physiological model accounting for transmembrane ion fluxes was applied to simulate the RBC physiological state to address the problem quantitatively. Instead of assuming the plasma membrane to be impermeable to ions, the dynamic nature of cell physiological state and capacity of cells to perform acute cell volume regulation by increasing passive cation plasma membrane permeability is recognized. It is shown that the RBC plasma membrane contains ion pathways capable of supporting a several-order increase in catio permeability, facilitating overall salt loading. The obtained picture, consistent with general physiology, predicts the experimentally observed RBC behavior. Although treated in theory, evidence supporting the proposed theory is provided, and the high explanatory power of the proposed idea is demonstrated. While focused on human RBCs, this study offers a general mechanism for the osmotic injury of cells exposed to hypertonic solutions - conditions relevant to cell cryopreservation - since many cells possess cell volume regulation mechanisms. Implications of the proposed hypothesis explaining the cryoprotective mechanism of common cryoprotectants are discussed, and additives targeting ion transport pathways are suggested as novel cryoprotectants.
Disruption of aPlasmodium falciparumpatatin-like phospholipase delays male gametocyte exflagellation
Authors: Pietsch Emma , Niedermüller Korbinian , Gilberger Tim-Wolf , Burda Paul-Christian 
Published: April 2023

ABSTRACTAn essential process in transmission of the malaria parasite to theAnophelesvector is the conversion of mature gametocytes into gametes within the mosquito gut, where they egress from the red blood cell (RBC). During egress, male gametocytes undergo exflagellation, leading to the formation of eight haploid motile microgametes, while female gametes retain their spherical shape. Gametocyte egress depends on sequential disruption of the parasitophorous vacuole membrane and the host cell membrane. In other life cycle stages of the malaria parasite, phospholipases have been implicated in membrane disruption processes during egress, however their importance for gametocyte egress is relatively unknown. Here, we performed comprehensive functional analyses of six putative phospholipases for their role during development and egress ofPlasmodium falciparumgametocytes. We localize two of them, the prodrug activation and resistance esterase (PF3D7_0709700) and the lysophospholipase 1 (PF3D7_1476700), to the parasite plasma membrane. Subsequently, we show that disruption of most of the studied phospholipase genes does neither affect gametocyte development nor egress. The exception is the putative patatin-like phospholipase PF3D7_0924000, whose gene deletion leads to a delay in male gametocyte exflagellation, indicating an important, albeit not essential, role of this enzyme in male gametogenesis.
Diet and injection, our recommendation to characterizeClavibacter michiganensis- tomato interactions
Authors: Brochu Anne-Sophie , Durrivage Jeanne , Torres Dagoberto , Pérez-López Edel 
Published: April 2023

ABSTRACTTomato (Solanum lycopersicumL.) is one of the most important vegetables in the world. Its extensive cultivation has made this plant the target of many viral, fungal, and bacterial diseases. Among them, the bacterial canker of tomato caused byClavibacter michiganensis(Cm) has been named one of the most devastating diseases affecting the tomato industry worldwide. It can significantly reduce the yields and profitability of this crop. One of the big challenges we found when working withCmand trying to characterize the virulence of different isolates was the lack of a consensus methodology to inoculate tomato plants, how to fertilize them and characterizeCmvirulence. This research aimed to identify an artificial inoculation method to induce bacterial canker on tomato plants in greenhouse conditions to homogenize the results of different studies withCm. We compared two inoculation methods, including the scalpel and syringe method with two levels of fertilization, low and high fertilization. After evaluating several variables like the percentage of necrotic leaves and the height of the plants, the results showed that the syringe inoculation with low fertilization was the most effective inoculation method allowing the development of a multilevel scale that can be used to study the interaction between tomato plants andCmisolates.
A transposase-derived gene required for human brain development
Authors: Zapater Luz Jubierre , Lewis Sara A. , Gutierrez Rodrigo Lopez , Yamada Makiko , Rodriguez-Fos Elias , Planas-Felix Merce , Cameron Daniel , Demarest Phillip , Nabila Anika , Mueller Helen , Zhao Junfei , Bergin Paul , Reed Casie , Chwat-Edelstein Tzippora , Pagnozzi Alex , Nava Caroline , Bourel-Ponchel Emilie , Cornejo Patricia , Dursun Ali , Özgül R. Köksal , Akar Halil Tuna , Maroofian Reza , Houlden Henry , Cheema Huma Arshad , Anjum Muhammad Nadeem , Zifarelli Giovanni , Essid Miriam , Hafsa Meriem Ben , Benrhouma Hanene , Montoya Carolina Isabel Galaz , Proekt Alex , Zhao Xiaolan , Socci Nicholas D. , Hayes Matthew , Bigot Yves , Rabadan Raul , Torrents David , Kleinmann Claudia L , Kruer Michael C. , Toth Miklos , Kentsis Alex 
Published: April 2023

AbstractDNA transposable elements and transposase-derived genes are present in most living organisms, including vertebrates, but their function is largely unknown. PiggyBac Transposable Element Derived 5 (PGBD5) is an evolutionarily conserved vertebrate DNA transposase-derived gene with retained nuclease activity in human cells. Vertebrate brain development is known to be associated with prominent neuronal cell death and DNA breaks, but their causes and functions are not well understood. Here, we show that PGBD5 contributes to normal brain development in mice and humans, where its deficiency causes disorder of intellectual disability, movement, and seizures. In mice, Pgbd5 is required for the developmental induction of post-mitotic DNA breaks and recurrent somatic genome rearrangements. In the brain cortex, loss of Pgbd5 leads to aberrant differentiation and gene expression of distinct neuronal populations, including specific types of glutamatergic neurons, which explains the features of PGBD5 deficiency in humans. Thus, PGBD5 might be a transposase-derived enzyme required for brain development in mammals.One-Sentence SummaryPiggyBac Transposable Element Derived 5 (PGBD5) is required for brain development in humans and mice through genetic and epigenetic mechanisms.
EBF1 limits the numbers of cochlear hair and supporting cells and forms the scala tympani and spiral limbus during inner ear development
Authors: Kagoshima Hiroki , Ohnishi Hiroe , Yamamoto Ryosuke , Yasumoto Akiyoshi , Tona Yosuke , Nakagawa Takayuki , Omori Koichi , Yamamoto Norio 
Published: April 2023

AbstractEarly B-cell factor 1 (EBF1) is a basic helix-loop-helix transcription factor essential for the differentiation of various tissues. Our single-cell RNA sequencing data suggest thatEbf1is expressed in the sensory epithelium of the inner ear. Here, we found that theEbf1gene and its protein are expressed in the prosensory domain of the inner ear, medial region of the cochlear floor, inner ear mesenchyme, and cochleo-vestibular ganglion.Ebf1deletion results in incomplete formation of the spiral limbus and scala tympani, increased number of cells in the organ of Corti and Kölliker’s organ, and aberrant course of the spiral ganglion axons. The inner ear-specific deletion ofEbf1revealed that scala tympani formation depended onEbf1expressed in the otic mesenchyme.Ebf1deletion in the cochlear epithelia caused the proliferation of SOX2-positive cochlear cells at E13.5, indicating that EBF1 suppresses the proliferation of the prosensory domain and cells of Kölliker’s organ to facilitate the development of appropriate numbers of hair and supporting cells. Furthermore, mice with deletion of cochlear epithelium-specificEbf1showed poor postnatal hearing function. Our results suggest thatEbf1is essential for normal auditory function in mammals.
Neural dynamics of predictive timing and motor engagement in music listening
Authors: Zalta Arnaud , Large Edward W. , Schön Daniele , Morillon Benjamin 
Published: April 2023

AbstractWhy do humans spontaneously dance to music? To test the hypothesis that motor dynamics reflect predictive timing during music listening, we built melodies with varying degrees of rhythmic predictability. Magnetoencephalography data showed that while auditory regions track the rhythm of melodies, intrinsic neural dynamics at delta (1.4 Hz) and beta (20-30 Hz) rates in the dorsal auditory pathway embody the experience of groove. Critically, neural dynamics are organized along this pathway in a spectral gradient, with the left sensorimotor cortex acting as a hub coordinating groove-related delta and beta activity. Combined with predictions of a neurodynamic model, this indicate that spontaneous motor engagement during music listening is a manifestation of predictive timing effected by interaction of neural dynamics along the dorsal auditory pathway.One-Sentence SummaryInteracting neural dynamics along the dorsal auditory pathway effect the experience of groove during music listening.
Effect of substitution of wheat flour with chickpea flour on their physico-chemical characteristics
Authors: Sidhu Jiwan S. , Zafar Tasleem , Almusallam Abdulwahab , Ali Muslim , Al-Othman Amani 
Published: April 2023

PurposeThe major objective of this research work was to evaluate various physico-chemical characteristics, such as, chemical composition, antioxidant capacity, objective color and texture profile analysis (TPA) of the wheat flour/chickpea flour (CF) blends, so that nutritious baked products could be consumed by the type-2 diabetic persons.Design/methodology/approachWholegrain wheat flour (WGF) and white wheat flour (WWF) were substituted with CF at 0 to 40% levels. These wheat flour/CF blends were analyzed for proximate composition, the prepared dough and baked breads were tested for objective color, antioxidant capacity as trolox equivalent antioxidant capacity (TEAC), malondialdehyde (MDA) and total phenolic content (TPC) and TPA.FindingsWGF had the highest TEAC (117.42 mM/100g) value, followed by WWF (73.98 mM/100g) and CF (60.67 mM/100g). TEAC, MDA and TPC values varied significantly among all the three flour samples.Research limitations/implicationsInclusion of whole chickpea (without dehulling) flour in such type of blends would be another interesting investigation during the future research studies.Practical implicationsThese research findings have a great potential for the production of these baked products for human consumption on an industrial scale.Social implicationsProduction of breads using wheat flour and CF blends would benefits the consumers.Originality/valueProduction of Arabic and pan breads using wheat flour and CF blends would, therefore, combine the benefits of both the needed proteins of plant origin and the health-promoting bioactive compounds, in a most sustainable way for the consumers.
Emotions, perceived knowledge and food behaviors during stressful periods
Authors: Xie Hu , Veeck Ann , Yu Hongyan , Zhu Hong 
Published: April 2023

PurposeThis paper aims to examine how emotions affect consumers' food choices and food preparation activities during stressful periods, using the context of the coronavirus disease 2019 (COVID-19) outbreak in China.Design/methodology/approachThis study used an online survey, with a sample of 1,050 individuals from 32 regions in China. Multi-regression and mediation models were used to test the relationships among perceived knowledge, emotions and food behaviors.FindingsThe results show that positive emotions positively affect healthy food consumption and engagement in food preparations. In contrast, negative emotions contribute to an increase in indulgent food consumption and quick-and-easy meal preparations. Increased knowledge of the current situation can enhance positive emotions and thus promote healthy food behaviors. Lacking knowledge may result in unhealthy food behaviors through negative emotions.Originality/valueThis study contributes to the understanding of emotions and food behaviors by examining the effects of both negative and positive emotions in the general population, exploring a wider constellation of food behaviors and identifying perceived knowledge as an important antecedent to emotions' effects on food behaviors. Implications for consumers and public policy are offered.
Industrialized house building productivity growth
Authors: Stehn Lars , Jimenez Alexander 
Published: May 2023

Purpose
The purpose of this paper is to understand if and how industrialized house building (IHB) could support productivity developments for housebuilding on project and industry levels. The take is that fragmentation of construction is one explanation for the lack of productivity growth, and that IHB could be an integrating method of overcoming horizontal and vertical fragmentation.

Design/methodology/approach
Singe-factor productivity measures are calculated based on data reported by IHB companies and compared to official produced and published research data. The survey covers the years 2013–2020 for IHB companies building multi-storey houses in timber. Generalization is sought through descriptive statistics by contrasting the data samples to the used means to control vertical and horizontal fragmentation formulated as three theoretical propositions.

Findings
According to the results, IHB in timber is on average more productive than conventional housebuilding at the company level, project level, in absolute and in growth terms over the eight-year period. On the company level, the labour productivity was on average 10% higher for IHB compared to general construction and positioned between general construction and general manufacturing. On the project level, IHB displayed an average cost productivity growth of 19% for an employed prefabrication degree of about 45%.

Originality/value
Empirical evidence is presented quantifying so far perceived advantages of IHB. By providing analysis of actual cost and project data derived from IHB companies, the article quantifies previous research that IHB is not only about prefabrication. The observed positive productivity growth in relation to the employed prefabrication degree indicates that off-site production is not a sufficient mean for reaching high productivity and productivity growth. Instead, the capabilities to integrate the operative logic of conventional housebuilding together with logic of IHB platform development and use is a probable explanation of the observed positive productivity growth.
Micro-credentials and reflections on higher education
Authors: Orman Rukiye , Şi̇mşek Elif , Kozak Çakır Meryem Ayşegül 
Published: April 2023

PurposeMicro-credentials, which have been widely used in re-skilling and skills development, provide advantages for improvement in the professional career. As a complementary approach to career and professional development in formal education, it can be said that micro-credentials are preferred for supporting professional development and/or to acquiring new skills to find a job in a new field (Fisher and Leder, 2022). This study aims to provide a detailed perspective on conceptual framework and the use of micro-credentials in higher education.Design/methodology/approachMany questions have arisen regarding micro-credentials, applicability, quality assurance, inclusion in formal educational settings and how to include them in traditional education systems, especially in higher education institutions. Oliver (2019) emphasizes that there is little research on micro-credentials or their derivatives in the literature. There is still uncertainty on micro-credential on behalf of students, employers or employees. In addition, although there are studies on micro-credentials in the USA, Europe and Australia, there is very little research around countries such as Turkey (Yilik, 2021). This study discusses the use of micro-credentials in the world and their reflections on higher education.FindingsAs it is shown in this article, micro-credentials have the potential to challenge or complement traditional ways of how learning is taking place, understood, recognized and certified. Research on micro-credentials is still in its infancy and could benefit a lot from more research. Especially research on employers' recognition and appreciation of digital credentials needs to be studied. Also, empirically confirming propositions of literature on micro-credentials could be beneficial.Originality/valueThis study is original in terms of discussing the use of micro-credential in the world and their reflections on higher education and presenting suggestions on this subject.
Evaluation of an artificial intelligence project in the software industry based on fuzzy analytic hierarchy process and complex adaptive systems
Authors: Chang Tsung-Sheng 
Published: April 2023

PurposeArtificial intelligence (AI) is the most progressive commodity among current information system applications. In-house development and sales of beneficial products are difficult for many software development and service companies (SDSCs). SDSCs have some implicit concerns about implementing AI software development due to the complexity of AI technology; they require an evaluation framework to avoid development failure. To fill the void, this study identified the factors influencing SDSCs when developing AI software development.Design/methodology/approachBased on complex adaptive systems theory, three aspects were developed as the main factors of hierarchy, namely, employees' capabilities, environmental resources and team capabilities. Fuzzy analytic hierarchy process (FAHP) was used to assess the SDSCs' attitude. Based on SDSCs, attitudes toward implementing AI software projects were collected to calculate the hierarchy of factors.FindingsThe outcome of FAHP is used as understanding the key factors of SDSCs for selecting an AI software project, toward the improvement of overall project planning. Employees' stress resistance was considered as a priority for the project, although professional AI skills and resources were also important.Originality/valueThis study suggested three variables developed using complex adaptive systems. This study contributes to a better understanding of the critical aspects of developing AI software projects in SDSCs. The study's findings have practical and academic implications for SDSCs and subsequent academic development, broadening the scope of AI software development research.
Reliability evaluation on SRG with full-bridge power converter considering thermal stress
Authors: Yang Fan , Chen Hao , Xu Shuai 
Published: May 2023

Purpose
Quantitative reliability analysis can effectively identify the time the driving system needs to be maintained. Then, the potential safety problems can be found, and some catastrophic failures can be effectively prevented. Therefore, this paper aims to evaluate the reliability of the switched reluctance generator (SRG) driving system.

Design/methodology/approach
In this paper, a method considering different thermal stresses and fault tolerance capacity is proposed to analyze the reliability of an SRG. A full-bridge power converter (FBPC) instead of the asymmetric half-bridge power converter (AHBPC) is adopted to drive the SRG system. First, the primary fault modes of the SRG system are introduced, and a fault criterion is proposed to determine whether the system fails. Second, the thermal circuit model of the converter is established to quickly and accurately obtain the junction temperature of the devices. At last, the Markov models of different levels are established to evaluate the reliability of the system.

Findings
The results show that the two-level Markov model is the most suitable when compared to the static model and the one-level Markov model.

Originality/value
The driving system of SRG will be more reliable after the reliability of the system is evaluated by the Markov model. At the same time, an FBPC is adopted to drive the SRG. The FBPCs have the advantages of fewer switching devices, higher integration and lower cost. The proposed driving strategy of the FBPC avoids the current reversal and the generation of dead zone time, which has the advantage of reliable operation. In addition, a precise thermal circuit model of the FBPC is proposed, and the junction temperature of each device can be obtained, respectively.