bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between adverse childhood experiences and peripartal pain experience
Authors: Zehetmeier Katharina Fiona , Fröhlich Melissa Kathrin , Schilder Andreas , Lis Stefanie , Schmahl Christian , Treede Rolf-Detlef , Sütterlin Marc 
Published: February 2023

Abstract
Adverse childhood experiences (ACEs) are associated with altered ongoing and evoked pain experiences, which have scarcely been studied for the peripartum period. We aimed to investigate how ACEs affect pain experience in pregnancy and labor. For this noninterventional trial with a short-term follow-up, pregnant women were divided into a trauma group (TG) with ACEs (n = 84) and a control group (CG) without ACEs (n = 107) according to the Childhood Trauma Questionnaire. Pain experience in pregnancy and labor was recorded by self-report and the German Pain Perception Scale. Pain sensitivity prepartum and postpartum was assessed by Quantitative Sensory Testing and a paradigm of conditioned pain modulation (CPM), using pressure pain thresholds (PPTs) and a cold pressor test. The TG showed higher affective and sensory scores for back pain and a more than doubled prevalence of preexisting back pain. Pelvic pain differences were nonsignificant. The TG also exhibited increased affective scores (1.71 ± 0.15 vs 1.33 ± 0.11), but not sensory scores for labor pain during spontaneous delivery. There were no group differences in prepartum pain sensitivity. While PPTs increased through delivery in the CG (clinical CPM), and this PPT change was positively correlated with the experimental CPM (r = 0.55), this was not the case in the TG. The association of ACEs with increased peripartal pain affect and heightened risk for preexisting back pain suggest that such women deserve special care. The dissociation of impaired clinical CPM in women with ACEs and normal prepartum experimental CPM implies at least partly different mechanisms of these 2 manifestations of endogenous pain controls.
Small cell lung cancer transformation after EGFR-TKIs treatment in lung adenocarcinoma: A case report and literatures review
Authors: Chen Xiaoli , Li Dongmei , Miao Kun , Shou Tao , Zhang Wenjing 
Published: February 2023

Rationale:
Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are commonly used in the treatment of advanced non-small cell lung cancer. However, patients will inevitably develop resistance to EGFR-TKIs in the long-term treatment process. In this paper, we report a case of small cell lung cancer transformation after EGFR-TKIs treatment in lung adenocarcinoma. We summarize the characteristics of this case and the treatment after transformation, and emphasized the repeat biopsy and dynamic monitoring its genetic mutation was necessary.

Patient concerns:
A 75-years-old man with no smoking history was admitted to our hospital with repeated cough and expectoration for 1 month and chest enhancement computed tomography showed paracbronchial soft tissue mass in the lower lobe of the left lung, which was considered to be central lung cancer.

Diagnoses:
The first pathological analysis of lung biopsy confirmed left lung adenocarcinoma and clinical stage was T3N3M1 IVA. In June 2021, the second bronchoscopic biopsy was performed, and pathology showed small cell neuroendocrine carcinoma in the left lung.

Interventions:
Gefitinib was given to patients when the first next generation sequence test showed EGFR L858 mutation. When the second next generation sequence test revealed EGFR T790M mutation, the patient received with osimertinib. The patient got 2 cycles chemotherapy of etoposide plus netaplatin when diagnosed with small cell lung cancer.

Outcomes:
Progression-free survival was only 8 months after gefitinib treatment. Moreover, the patient was insensitive to Oxitinib, and the disease progressed after 2 months of treatment with Oxitinib. Finally, he died of severe infection and hepatic failure after a diagnosis of small cell lung cancer.

Lessons:
Our case highlights that if a patient has rapid disease progression, increase of serum neuron-specific enolase, and TP53 and Rb1 inactivation during EGFR-TKIs treatment, we should be alert to the pathological type transformation to small cell lung cancer.
A large family of hereditary spherocytosis and a rare case of hereditary elliptocytosis with a novel SPTA1 mutation underdiagnosed in Taiwan: A case report and literature review
Authors: Shih Yu-Hung , Huang Ying-Chih , Lin Ching-Yeh , Lin Hsuan-Yu , Kuo Su-Feng , Lin Jen-Shiou , Shen Ming-Ching 
Published: February 2023

Rationale:
Hereditary spherocytosis (HS) has a defect in the vertically connected proteins on the cell membrane of red blood cells (RBC). Hereditary elliptocytosis (HE) has a defect in proteins that connect the cell membrane horizontally. We reported two families of RBC membrane disorders in Taiwanese, one was HS and the other was HE.

Patient concerns:
Case 1. A 19-year-old male student with chronic jaundice and splenomegaly. His mother, maternal uncle, grandmother, and many members of older generations also had splenomegaly and underwent splenectomy. Case 2. A 40-year-old man has experienced pallor and jaundice since the age of 20 and was found to have splenomegaly, and gall bladder stones in the older age. His younger sister also had pallor and jaundice for a long time.

Diagnoses:
In case 1, a peripheral blood smear showed 20% spherocytes. Eosin-5-maleimide labeled RBC by flow cytometry showed a result of 30.6 MCF (cutoff value: 45.5 MCF). He was diagnosed with HS. The gene analysis identified a heterozygous mutation with c.166A > G (p.Lys56Glu) in the SLC4A1 gene in this proband, his mother, and maternal uncle. In case 2, more than 40% of ellipsoid RBC present in the peripheral blood smear. He was diagnosed with HE. Genetic analysis of the SPTA1 gene identified a novel heterozygous exon2, c.86A > C, p.Gln29Prol mutation.

Interventions:
The two patients had compensated anemia, clinical follow-up instead of splenectomy was done.

Outcomes:
The two patients had normal daily activities and lives.

Lessons:
We reported two Taiwanese families, one was hereditary spherocytosis affected by a heterozygous mutation with c.166A > G (p.Lys56Glu) in SLC4A1, and the other was hereditary elliptocytosis caused by a novel heterozygous SPTA1 gene mutation, c. 86A > C, p.Gln29Prol. These 2 seemingly common hereditary red blood cell membrane protein defects induced by hemolysis are usually underdiagnosed or misdiagnosed.
Morbidity and Mortality in Neonates with Symptomatic Congenital Lung Malformation
Authors: Engwall-Gill Abigail J , Weller Jennine H , Salvi Pooja S , Penikis Annalise B , Sferra Shelby R , Rhee Daniel S , Solomon Daniel G , Kunisaki Shaun M 
Published: February 2023

Objective:
Neonatal resection is the mainstay treatment of children presenting with symptomatic congenital lung malformations (CLM) at birth. The objective of this study was to evaluate risk factors for increased morbidity and mortality after neonatal CLM resection using a large multicenter database.

Study Design:
Retrospective review of the Pediatric Health Information System database was performed. Children with a symptomatic CLM managed by lung resection before 30 days of age were included (2016-2021). Primary outcome measures were postoperative respiratory complications and any complication, including death.

Results:
Of 1791 CLM patients identified, 256 (14%) underwent neonatal resection for symptomatic disease. Pathology included 123 (48%) congenital pulmonary airway malformations, 24 (10%) bronchopulmonary sequestrations (BPS), 5 (2%) congenital lobar emphysema, 16 (6%) hybrid, and 88 (34%) unclassified lesions. Preoperative mechanical ventilation and extracorporeal membrane oxygenation (ECMO) were employed in 149 (58.2%) and 17 (6.7%) of cases, respectively. The median age at resection was 6.5 days (IQR 2-23). Postoperatively, 25 (10%) required mechanical ventilation for ≥48 hrs, 3 (1%) continued ECMO, and 3 (1%) required ECMO rescue. Overall respiratory complication rate was 34% (n=87), any complication was 51% (n=130), median postoperative length of stay (LOS) was 20 days (IQR 9, 52), and mortality rate was 14.5% (n=37). Birthweight was inversely correlated with complication risk (IRR: 0.55, CI: 0.36-0.83, p=0.006). Cardiac structural anomalies were associated with a 21 day longer postoperative LOS (CI: 6-35, p=0.006) and 2.2 times increased risk of any complication (CI 1.18-4.02, p=0.014).

Conclusion:
In this large multicenter study, ECMO use and mortality are relatively uncommon among neonates undergoing lung resection for a symptomatic CLM. However, postoperative morbidity remains high, particularly in those with cardiac structural disease.
Quantifying the impact of interventions againstPlasmodium vivaxmalaria: a model for country-specific use
Authors: Champagne C. , Gerhards M. , Lana J. , Menach A. Le , Pothin E. 
Published: February 2023

AbstractIn order to evaluate the impact of various intervention strategies onPlasmodium vivaxdynamics, we introduce a simple mathematical model that can be easily adapted to country-specific data. The model includes case management, vector control, mass drug administration and reactive case detection interventions and is implemented in both deterministic and stochastic frameworks. It is available as an R package to enable users to calibrate and simulate it with their own data. By simulating and comparing the impact of various intervention combinations on malaria risk and burden, this model can a useful tool for strategic planning, implementation and resource mobilization.
Dual-systems models of the genetic architecture of impulsive personality traits: Neurogenetic evidence of distinct but related factors
Authors: Miller Alex P. , Gizer Ian R. 
Published: February 2023

AbstractBackgroundDual-systems models provide a parsimonious framework for understanding the interplay between cortical and subcortical brain regions relevant to impulsive personality traits (IPTs) and their associations with psychiatric disorders. Despite recent developments in multivariate analysis of genome-wide association studies (GWAS), molecular genetic investigations of these models have not been conducted.MethodsUsing extant IPT GWAS, we conducted confirmatory genomic structural equation models (GenomicSEM) to empirically evaluate dual-systems models of the genetic architecture of IPTs. Genetic correlations between results of multivariate GWAS of dual-systems factors and GWAS of relevant cortical and subcortical neuroimaging phenotypes (regional/structural volume, cortical surface area, cortical thickness) were calculated and compared.ResultsEvaluation of GenomicSEM model fit indices for dual-systems models suggested that these models highlight important sources of shared and unique genetic variance between top-down and bottom-up constructs. Specifically, a dual-systems genomic model consisting of sensation seeking and lack of self-control factors demonstrated distinct but related sources of genetic influences (rg=.60). Genetic correlation analyses provided evidence of differential associations between dual-systems factors and cortical neuroimaging phenotypes (e.g., lack of self-control negatively associated with cortical thickness, sensation seeking positively associated with cortical surface area). However, no significant associations were observed for subcortical phenotypes inconsistent with hypothesized functional localization of dual-systems constructs.ConclusionsDual-systems models of the genetic architecture of IPTs tested here demonstrate evidence of shared and unique genetic influences and associations with relevant neuroimaging phenotypes. These findings emphasize potential advantages in utilizing dual-systems models to study genetic influences for IPTs and transdiagnostic associations with psychiatric disorders.
Quantifying the rate and magnitude of the Omicron outbreak in China after sudden exit from ‘zero-COVID’ restrictions
Authors: Goldberg Emma E. , Lin Qianying , Romero-Severson Ethan O. , Ke Ruian 
Published: February 2023

AbstractIn late 2022, China transitioned from a strict ‘zero-COVID’ policy to rapidly abandoning nearly all interventions and data reporting. This raised great concern about the presumably-rapid but undisclosed spread of the SARS-CoV-2 Omicron variant in a very large population of very low pre-existing immunity. A quantitative understanding of the epidemic dynamics of COVID-19 during this period is urgently needed. Here, by modeling a combination of case count and survey data, we show that Omicron spread extremely fast, at a rate of 0.42/day (95% CrI: [0.35, 0.51]/day) after the full exit from zero-COVID policies on Dec. 7, 2022. Consequently, we estimate that the vast majority of the population (97%, 95% CrI [95%, 99%]) was infected during December, with the nation-wide epidemic peaking on Dec. 23. Overall, our results highlight the extremely high transmissibility of the variant and the importance of proper design of intervention exit strategies to avoid large infection waves.
Integrative single-cell characterization of frugivory adaptations in the bat kidney and pancreas
Authors: Gordon Wei E. , Baek Seungbyn , Nguyen Hai P. , Kuo Yien-Ming , Bradley Rachael , Galazyuk Alex , Lee Insuk , Ingala Melissa R. , Simmons Nancy B. , Schountz Tony , Cooper Lisa Noelle , Georgakopoulos-Soares Ilias , Hemberg Martin , Ahituv Nadav 
Published: February 2023

AbstractFrugivory evolved multiple times in mammals, including bats. However, the cellular and molecular components driving it remain largely unknown. Here, we used integrative single-cell sequencing on insectivorous and frugivorous bat kidneys and pancreases and identified key cell population, gene expression and regulatory element differences associated with frugivorous adaptation that also relate to human disease, particularly diabetes. We found an increase in collecting duct cells and differentially active genes and regulatory elements involved in fluid and electrolyte balance in the frugivore kidney. In the frugivorous pancreas, we observed an increase in endocrine and a decrease in exocrine cells and differences in genes and regulatory elements involved in insulin regulation. Combined, our work provides novel insights into frugivorous adaptation that also could be leveraged for therapeutic purposes.
Extra-islet expression of islet antigen boosts T-cell exhaustion to prevent autoimmune diabetes
Authors: Selck Claudia , Jhala Gaurang , De George David , Kwong Chun-Ting J. , Christensen Marie K. , Pappas Evan , Liu Xin , Ge Tingting , Trivedi Prerak , Kallies Axel , Thomas Helen E. , Kay Thomas W.H. , Krishnamurthy Balasubramanian 
Published: February 2023

AbstractPersistent antigen exposure results in the differentiation of functionally impaired, also termed exhausted, T cells which are maintained by a distinct population of precursors of exhausted T (TPEX) cells. T cell exhaustion is well studied in the context of chronic viral infections and cancer, but it is unclear if and how antigen-driven T cell exhaustion controls progression of autoimmune diabetes and whether this process can be harnessed to prevent diabetes. Using non-obese diabetic (NOD) mice, we show that some CD8+ T cells specific for the islet antigen, islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP) displayed terminal exhaustion characteristics within pancreatic islets but were maintained in the TPEXcell state in peripheral lymphoid organs. To examine the impact of antigen on T cell exhaustion in diabetes, we generated transgenic NOD mice with inducible IGRP expression in peripheral antigen presenting cells. Antigen exposure in the extra-islet environment induced severely exhausted IGRP-specific T cells with reduced ability to produce IFNγ, which protected these mice from diabetes. Our data demonstrate that T cell exhaustion induced by delivery of antigen can be harnessed to prevent autoimmune diabetes.
Impacts of invasive fishes on ghost frog tadpoles
Authors: van Blerk Daniel , Melotto Andrea , Pegg Josie , Measey John 
Published: February 2023

AbstractGlobal amphibian populations are declining, and invasive fish are known to impact many threatened species. South Africa has both a biodiverse amphibian fauna and a rich history of invasive fish introductions with high establishment success. Studies have identified some preliminary evidence for negative impacts of invasive salmonids on two ghost frog species (Anura: Heleophrynidae). This study aims to investigate whether these negative impacts previously reported are reflected across a broader scale. Tadpoles of two species,Heleophryne regisandH. purcelliwere sampled in the presence and absence of invasive fish at 112 sites across 26 streams in the Western Cape province of South Africa. A generalised linear mixed model showed invasive fish to have the most significant negative effect explaining tadpole abundance. Mean tadpole abundance decreases by 18 times in the presence of invasive fish. Environmental variables with significant effects on tadpole abundance include pH, oxygen saturation, temperature, stream depth and non-native riparian vegetation type. We can define an environmental niche of ghost frog tadpoles which does not differ between species. We conclude that invasive fish have significant negative impacts on ghost frog tadpole abundance, but the effect of the environment should not be overlooked in amphibian conservation planning and invasive species management decisions. This study supports the removal of invasive fish and alien vegetation to improve the conservation of ghost frogs.
An expanded 2-pyridinecarboxaldehyde (2PCA)-based chemoproteomics toolbox for probing protease specificity
Authors: Bridge Haley N. , Frazier Clara L. , Weeks Amy M. 
Published: February 2023

SummaryProteomic profiling of protease-generated N termini, or N terminomics, provides key insights into protease function and specificity. However, current N terminomics technologies have sequence limitations or require specialized synthetic reagents for N-terminal peptide isolation. Here, we introduce an expanded N terminomics toolbox that is based on 2-pyridinecarboxaldehyde (2PCA) reagents. These tools enable efficient enrichment of protein N termini by combining selective N-terminal biotinylation using 2PCA reagents with chemically cleavable linkers for N-terminal peptide recovery. By incorporating a commercially available alkyne-modified 2PCA in combination with Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC), our strategy eliminates the need for chemical synthesis of N-terminal probes. Using these reagents, we developed PICS2 (Proteomic Identification ofCleavageSites with2PCA reagents) to profile the specificity of subtilisin/kexin-type proprotein convertases (PCSKs). We also implemented CHOPPER (Chemical enrichmentOfProtease substrates withPurchasable,ElutableReagents) for global sequencing of apoptotic proteolytic cleavage sites. Based on their broad applicability and ease of implementation, PICS2 and CHOPPER are useful tools that will advance our understanding of protease biology.
Znf598-mediated Rps10/eS10 ubiquitination contributes to the ribosome ubiquitination dynamics during zebrafish development
Authors: Ugajin Nozomi , Imami Koshi , Takada Hiraku , Ishihama Yasushi , Chiba Shinobu , Mishima Yuichiro 
Published: February 2023

AbstractRibosome is a translational apparatus that comprises about 80 ribosomal proteins and four rRNAs. Recent studies reported that ribosome ubiquitination is crucial for translational regulation and ribosome-associated quality control (RQC). However, little is known about the dynamics of ribosome ubiquitination under complex biological processes of multicellular organisms. To explore ribosome ubiquitination during animal development, we generated a zebrafish strain that expresses a FLAG-tagged ribosomal protein Rpl36/eL36 from its endogenous locus. We examined ribosome ubiquitination during zebrafish development by combining affinity purification of ribosomes fromrpl36-FLAG zebrafish embryos with immunoblotting analysis. Our findings showed that ubiquitination of ribosomal proteins dynamically changed as development proceeded. We also showed that during zebrafish development, the ribosome was ubiquitinated by Znf598, an E3 ubiquitin ligase that activates RQC. Ribosomal protein Rps10/eS10 was found to be a key ubiquitinated protein during development. Furthermore, we showed that Rps10/eS10 ubiquitination-site mutations reduced the overall ubiquitination pattern of ribosome. These results demonstrate the complexity and dynamics of ribosome ubiquitination during zebrafish development.
Vampyroteuthis pseudoinfernalissp. nov.: the second extant widespread deep sea squid species of Vampyromorpha (Cephalopoda: Coleoidea)
Authors: Qiu Dajun , Liu Bilin , Guo Yupei , Lakmini Wijesooriya A.S.W. , Tan Yehui , Li Gang , Ke Zhixin , Li Kaizhi , Huang Liangmin 
Published: February 2023

AbstractThe vampire squid,Vampyroteuthis infernalisChun, 1903, is currently the only extant species in the family Vampyroteuthidae Chun, 1903. However, specimens from the Gulf of Guinea, Africa, and California suggested the possibility of additional taxa. Here, we report the second species ofVampyroteuthis, collected from the South China Sea, China, which differs fromV. infernalisby the tail shape, the lower beak, and the phylogenetic analysis of mitochondrial COI and nuclear large subunit ribosomal DNA (LSU) sequences:V. infernalispresent by the lacking of the tail, photophores located approximately one-third of the points between the fins and end, and the lower beak with a broad, short wing;V. pseudoinfernalisQiu, Liu & Huang,sp. nov.present by an acuate tail, a pair of photophores located at the midpoints between the fins and tail, and a lower beak with a broad, elongate wing.
Macrophage epigenetic memories of early life injury drive neonatal nociceptive priming
Authors: Dourson Adam J. , Fadaka Adewale O. , Warshak Anna M. , Paranjpe Aditi , Weinhaus Benjamin , Queme Luis F. , Hofmann Megan C. , Evans Heather M. , Donmez Omer A. , Forney Carmy , Weirauch Matthew T. , Kottyan Leah C. , Lucas Daniel , Deepe George S. , Jankowski Michael P. 
Published: February 2023

AbstractThe developing peripheral nervous and immune systems are functionally distinct from adults. These systems are vulnerable to early life injury, which influences outcomes related to nociception following subsequent injury later in life (i.e., “neonatal nociceptive priming”). The underpinnings of this phenomenon are largely unknown, although previous work indicates that macrophages are epigenetically trained by inflammation and injury. We found that macrophages are both necessary and partially sufficient to drive neonatal nociceptive priming possibly due to a long-lasting epigenetic remodeling. The p75 neurotrophic factor receptor (NTR) was an important effector in regulating neonatal nociceptive priming through modulation of the inflammatory profile of rodent and human macrophages. This “pain memory” was long lasting in females and could be transferred to a naïve host to alter sex-specific pain-related behaviors. This study reveals a novel mechanism by which acute, neonatal post-surgical pain drives a peripheral immune-related predisposition to persistent pain following a subsequent injury.Graphical Abstract