bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bio-based poly(butylene 2,5-thiophenedicarboxylate-co-butylene 4,4'-biphenyldicarboxylate) with excellent ultraviolet shielding properties and barrier properties
Authors: Liang Yin , Wang Guoqiang , Wang Bo , Zhou Guangyuan 
Published: September 2023

Abstract
To improve the glass transition temperature and ultraviolet shielding properties of poly(butylene 2,5-thiophenedicarboxylate) (PBTF), a series of poly(butylene 2,5-thiophenedicarboxylate-co-butylene 4,4'-biphenyldicarboxylate) (PBTFBs) were synthesized from 2,5-thiophenedicarboxylic acid (TDCA), 1,4-butanediol (BDO), and dimethyl 4,4'-biphenyldicarboxylate (BDD). The composition and molecular weights of PBTFBs were investigated by 1H NMR, .13C NMR and GPC. The results showed that the glass transition temperature of PBTFBs gradually increased, and the thermogravimetric analysis results also showed that the thermal stability gradually increased with increasing the BDD content. Compared with PBTF, the polyester with 40% butylene 4,4'-biphenyldicarboxylate unit (PBTFB40) showed a higher Young's modulus (1166.8 MPa) and tensile strength (25.8 MPa). In addition, the ultraviolet (UV) shielding ability of PBTF was greatly enhanced, and the UV transmittance at 350 nm decreased from 19.66% (PBTF) to 0.29% (PBTFB40). The average transmittance at wavelengths of 320–400 nm decreased from 26.25–3.71%. The gas permeability coefficient of CO2 decreased from 0.3120 to 0.0321. Because the biphenyl structure was successfully introduced into PBTF, the rigidity of the chain segment was improved, and excellent thermal stability, mechanical properties and UV shielding properties were obtained. PBTFB40 has potential application in outdoor products.
Tracking induced pluripotent stem cells differentiation with a fluorescent genetically encoded epigenetic probe
Authors: Stepanov Afanasii I. , Shuvaeva Alexandra A. , Putlyaeva Lidia V. , Galiakberova Adelya A. , Pronina Valeriya , Dylov Dmitry V. , Terskikh Alexey V. , Lukyanov Konstantin A. , Gurskaya Nadya G. 
Published: September 2023

Abstract
Epigenetic modifications (methylation, acetylation, etc.) of core histones play a key role in regulation of gene expression. Thus, the epigenome changes strongly during various biological processes such as cell differentiation and dedifferentiation. Classical methods of analysis of epigenetic modifications such as mass-spectrometry and chromatin immuno-precipitation, work with fixed cells only. Here we present a genetically encoded fluorescent probe, MPP8-Green, for detecting H3K9me3, a histone modification associated with gene repression. This probe, based on the chromodomain of MPP8, allows for visualization of H3K9me3 epigenetic landscapes in single live cells. We used this probe to track changes in H3K9me3 landscapes during the differentiation of induced pluripotent stem cells (iPSCs) into induced neurons. Our findings revealed two major waves of global H3K9me3 reorganization during this process. The first wave occurred 16-24 hours after the induction, followed by a minor change on the second day. Then, on the third day, cells underwent another significant epigenetic change. By combining live visualization of epigenetic landscapes via genetically encoded probes and machine learning approaches, we can identify and characterize multiparametric epigenetic signatures of single cells during stem cell differentiation. This approach provides valuable insights into the dynamics of epigenetic changes during cellular differentiation processes.
Clinical characteristics and outcomes of psoriasis patients with COVID-19: a retrospective, multicenter cohort study in China
Authors: Liu Yanhua , Xu Zhongrui , Wang Gang , Yu Chen , Chen Aijun , Zhang Junling , Kang Xiaojing , Jiang Xian , Shi Chunrui , Shi Yuling , Liu Xiaoming , Li Fuqiu , Yang Bin , Huang Yongmei , Lv Chengzhi 
Published: September 2023

Abstract

Objective and design
Limited information is available on the impact of SARS-CoV-2 infection in psoriasis patients, and we aim to identify clinical factors associated with the prognosis of psoriasis following SARS-CoV-2 infection.
Subjects and methods
A retrospective, multicenter study was conducted between March and May 2023. Univariable and multivariable logistic regression analysis were employed to identify factors associated with COVID-19-related psoriasis outcomes. A total of 2371 psoriasis patients from 12 clinical centers were included in the study, with 2049 of them being infected with COVID-19.
Results
Among the infected group, individuals treated with biologics exhibited lower exacerbation rates compared to those receiving traditional systemic or non-systemic treatments (26.7% vs.39.8% vs.37.5%, P<0.001). Multivariable logistic regression analysis revealed that psoriasis progression with lesions (adjusted odds ratio[OR]=8.197, 95% confidence interval[CI]=5.685-11.820, compared to no lesions), hypertension (adjusted OR=1.582, 95%CI=1.068-2.343), traditional systemic (adjusted OR=1.887, 95%CI=1.263-2.818), and non-systemic treatment (adjusted OR=1.602, 95%CI=1.117-2.297) were associated with exacerbation of psoriasis after SARS-CoV-2 infection but not biologics (adjusted OR=0.931, 95%CI =0.680-1.274, compared to no treatment).
Conclusions
Biologics may reduce the risk of psoriasis exacerbation after SARS-CoV-2 infection, compared to traditional systemic and non-systemic treatments. The presence of existing psoriatic lesions and hypertension have been identified as significant risk factors for exacerbation after infection.
Robotic Versus Laparoscopic Retroperitoneal Resection of Large Adrenal Tumors: A Propensity Score-Matched Cohort Analysis
Authors: Gong Binbin , Huang Hao , Liu Ziwen , Xi Ping , Mei Ming , Ma Ming , Fu Bin , Wang Gongxian , Sun Ting , Xie Wenjie 
Published: September 2023

Abstract

Background
Laparoscopic adrenalectomy is the first-line treatment for benign adrenal tumors, but when the tumor is larger than 6 cm, its resection is controversial. The purpose of this study was to understand and compare the effects of robot-assisted and laparoscopic adrenalectomy on large adrenal tumors via a retroperitoneal approach.
Methods
We reviewed the perioperative data of patients who underwent robot-assisted and laparoscopic retroperitoneal adrenalectomy at the First Affiliated Hospital of Nanchang University between 2011 and 2020. After propensity score matching, the clinical data were analyzed by using the t test and chi-square test, as well as univariate and multivariate analysis.
Results
There is no significant difference between the two groups in operation time (RRA 191 ± 136min vs. 165 ± 83.8min, P = 0.158), postoperative complications (18.2% vs. 14.4%, P = 0.536), estimated blood loss (181 ± 167ml vs. 214 ± 341ml, P = 0.359), conversion rate (1.5% vs. 1.5%, P = 1.000) and postoperative hospital stay (6.55 ± 2.12 vs. 6.06 ± 2.39, P = 0.149). Multivariate analysis showed that larger tumors were associated with longer operative times. Female gender and postoperative complications were associated with longer hospital stays. BMI, conversion, history of abdominal surgery, and pathological type of patients affected the occurrence of postoperative complications.
Conclusion
it is safe and feasible for experienced surgeons to perform robotic adrenalectomy and laparoscopic adrenalectomy after the possibility of infiltration is ruled out on imaging before operation. However, when there is evidence of infiltration, we still recommend open surgery.
Allele-specific methylation of the PSA promoter in prostate cells: a new translational marker for the differential diagnosis of prostate cancer
Authors: Baryshev Mikhail , Maksimova Irina , Sasoveca Ilona 
Published: September 2023

Abstract
Background
DNA methylation is one of the mechanisms of epigenetic control of gene expression and a change in the intrinsic pattern can lead to various diseases and disorders. At the same time, this makes DNA methylation a disease-specific biomarker. Here, we show that the acquisition of biallelic methylation status or even biallelic lack of methylation by the PSA promoter is a characteristic feature of cancer cells, while the monoallelic distribution of CpG\CCWGG methylation in the PSA promoter is a hallmark of non-cancerous conditions.
Methods
We performed PCR bisulfite sequencing analysis of the proximal PSA promoter, which has six potential CpG and five CCWGG methylation marks that are targets of DNMTs and therefore have the ability to epigenetically influence gene expression and be a disease-specific biomarker. SNP G-158A, located in the AREI of the PSA promoter, was taken into account when genotyping prostate cell lines for allele-specific methylation analysis. To clarify the differences in PSA promoter methylation we applied RT-qPCR to estimate the level expression of the DNMTs by comparing that with data of gene expression in 54 tissues from GTEx RNA-seq of donors. A PCR cloning sequencing approach and detection of SNPs in exon 3 were used to identify biallelic expression of PSA in the LNCaP cell line.
Results
According to our study of prostate cancer cell lines, the CG/CCWGG methylation in PSA promoter has a great translational ability to be a disease-specific biomarker. Elevated PSA level along with monoallelic promoter methylation would reflect BPH or other non-malignant conditions, whereas a significantly elevated PSA level due to biallelic PSA expression and status of the unmethylated PSA promoter will be consistent with the PCa. The detection of biallelic PSA promoter methylation at the given PSA level will indicate the aggressive PCa. We propose that the CCWGG motif represents an allele-specific methylation signal, which is a novel functionality for this mark involved in the allele-specific methylation mechanism and/or imprinting, as well as its possible role in regulating monoallelic gene expression.
Significance
According to our study in prostate cell line models, the determination of allele-specific methylation in the PSA promoter can be translated into a disease-specific state and can refine PSA-based PCa testing.
Researchers' sex/gender identity influences how sex/gender question is investigated in neuroscience: An example from an OHBM meeting
Authors: Picó-Pérez Maria , Marco Elena Ábalos , Thurston Lindsey , Ambrosi Valerie , Genon Sarah , Bryant Katherine L. , Martínez Ana Belén , Ciccia Lu , Trujillo Anelis Kaiser 
Published: September 2023

Abstract
Gender inequality and diversity in STEM is a challenging field of research. Although the relation between the sex/gender of the researcher and the scientific research practices has been previously examined, less interest has been demonstrated towards the relation between sex/gender of the researcher and the research topic. Here, we examine, from a neurofeminist perspective, both questions: whether sex/gender diversity is related to the examination of sex/gender as a variable and whether sex/gender diversity is related to the topic of study. Using the database of submitted posters to the Organization of Human Brain Mapping 2022 annual conference, we identified abstracts examining a sex/gender-related research question. Among these target abstracts, we identified four analytical categories, varying in their degree of content-related complexity: (1) sex/gender as a covariate, (2) sex/gender as a binary variable for the study of sex/gender differences, (3) sex/gender with additional biological information, and (4) sex/gender with additional social information. Statistical comparisons between sex/gender of researcher and the research topics showed that the proportion of abstracts from Non-binaryor Other first authors compared to both Women and Men was lower for all submitted abstracts than for the target abstracts; that more researchers with sex/gender-identity other than Man implemented analytical category 4; and, for instance, that research involving cognitive, affective, and behavioural neuroscience more frequently fit into categories 4. Word cloud analysis confirmed the validity of the four exploratorily identified analytical categories. We conclude by discussing how raising awareness about contemporary neurofeminist approaches, including perspectives from the global south, is critical to neuroscientific and societal progress.
Amelioration of lithium stress in Vigna radiata (L.) R. Wilczek through the application of Plant Growth Promoting Bacteria
Authors: Hayyat Muhammad Umar. , Nawaz Rab , Siddiq Zafar , Khurshid Iram , Irfan Ali , Mekonnen Amare Bitew , Gaafar Abdel-Rhman Z. , Ahmed Zulkfil , Ali Sikandar , Bourhia Mohammed 
Published: September 2023

Abstract
Lithium is among the alkali metals which can significantly reduce plant growth and yield. In this study, different levels of lithium stress lithium were applied to Vigna radiata (Mung bean), an economically important crop, grown in earthen pots. Each treatment of lithium was compared with the plants inoculated with bacteria that is resistant to lithium. Growth and physiological parameters were measured during the study. The results showed a significant reduction in growth such as plant height, branch number, plant height, root, fresh and dry weight of shoot, seeds quantity per plant, and seeds’ weight, stomatal conductance, photosynthetic and transpiration rate as compared to the controlled and also the plants with Lithium treatment. However, for each treatment, there was a significant increase in the growth and physiological parameters among the plants inoculated with lithium-resistant bacteria. A relationship between lithium concentrations (grains and roots) and the biomass accumulation factor was positive and significant. The lithium level was significantly lower in the roots, shoots, and grains of the plants having bacterial inoculation in comparison with treatments of lithium only. This study showed that lithium can affect the physiological performance and growth of Vigna radiata. This reduction of growth can be significantly minimized by the application of lithium-resistant bacteria. The findings of this research work can be used to understand the lithium effects on the crops and also the utilization of microbes to ameliorate the lithium stress.
Temporal overlapping effect in pump-probe technique on the Rhodamine 6G with addition copper nanoparticle
Authors: khalid Saad , zoory muayyed , Ali Alaa 
Published: September 2023

Abstract
This study took place utilizing the laser "Pump-Probe" probe technique to investigate the properties of a rhodamine 6G laser dye. 532 nm wavelength laser was employed for excitation of the dye "pumping", while another laser with a wavelength of 657 nm was utilized for sensitization "probe". A copper nanomaterial with an average size of 43 nm has been added to the system. The green laser pulse width was modulated at various percentages, including 5%, 10%, 20%, 30%, 40%, and 50% of the original pulse width. The pulse utilized for the pumping process was divided into two components, one of the tow pulse was directed towards the dye, while optical path of the other component was altered by employing multiple mirrors, the modification in the optical path was implemented in order to establish an accurate delay time between the two pulses and control their overlap by extending the duration of the laser pulse. The transmittance laser intensity was determined both before and after the addition of the nanomaterial. Additionally, (FWHM) of the output laser pulse was computed. The wavelength of the output laser pulse was also determined through calculation. The study findings demonstrated a discernible impact resulting from the alteration of laser pulse width as well as the influence of the nanomaterial on the intensity and optical characteristics of the dye. This study is a component of a series of research endeavors focused on comprehending the interplay between nanomaterials and laser dyes as well as the resultant impact of these interactions on the characteristics of laser dyes.
Benefiting from change: range-expanding native thrushes in Argentina
Authors: Vazquez M. Soledad , Scorolli Alberto L. , Zalba Sergio M. 
Published: September 2023

Abstract
The widespread human activity on Earth exerts strong selective pressures on the species with which we coexist. Faced with constant environmental change, species respond with mechanisms of adaptation, expansion, retraction or displacement. Species that establish new populations outside their former range as a result of human-induced environmental change are called 'neonatives'. Many birds now appear to be expanding their ranges in response to habitat modification and climate change. In this study, we use data from three citizen science platforms (eBird, GBIF and VertNet) to document the range expansion of four thrushes in Argentina: Turdus amaurochalinus, T. chiguanco, T. falcklandii, and T. rufiventris. The genus has a history of recent expansion and seems to have benefited from forestation and the landscaping of towns and villages. We found that thrushes have expanded their range about 10-fold since their known distribution in the mid-1980s, expanding at an average linear rate of 35 km/year. In all cases, they also appear to have accelerated their expansion in the last 20 years. Turdus amaurochalinus, T. chiguanco and T. rufiventris have mainly expanded southwards, while T. falcklandii has expanded northwards. This expansion is likely to result in novel ecological interactions, affecting other native species in different ways, and also establishing new relationships with productive activities and urban environments. Thrushes provide an opportunity to understand the response of wild species to human change on the planet, and to design conservation strategies adapted to this new reality.
Application of multi-omics to investigate the effect of Pinus koraiensis cone essential oil on rumen methane emission, microbial community, and metabolites
Authors: Choi Youyoung , Lee Shin Ja , Kim Hyun Sang , Eom Jun Sik , Jo Seong Uk , Guan Le Luo , Lee Sung Sill 
Published: September 2023

Abstract
Background
Enteric methane (CH4) excreted by ruminants is a major source of anthropogenic greenhouse gas emissions in the global environment. Pinus koraiensis cone essential oil (PEO) contains functional compounds such as monoterpene hydrocarbons, which can directly affect the microbiota and their function in the rumen. Previously, we found that PEO oral administration during the growing phases of goats reduced CH4 emissions and was associated with the rumen prokaryotic microbiota. However, a more comprehensive analysis of the rumen microbiota and metabolites are needed. The objective was to elucidate the potential microbial features that underpin CH4 mitigation in goats using metataxonomics (prokaryotes, protozoa, and fungi) and metabolomics (rumen fluid and serum). Ten fattening Korean native goats were divided into two dietary groups: control (CON; basal diet without additives) and PEO (basal diet + 1.5 g/d of PEO), using a 2 × 2 crossover design for 11 weeks. Methane measurements were conducted every four consecutive days for 24–27 d.
Results
Oral administration of PEO reduced CH4 concentrations in the exhaled gas from eructation by 12.0–13.6% (P < 0.05). Although the microbiota structure, including prokaryotes, protozoa, and fungi, was not altered after PEO administration, MaAsLin2 analysis revealed that Selenomonas, Christensenellaceae R-7, and Anaerovibrio were enriched in the PEO group (Q < 0.1). Co-occurrence network analysis revealed that the Bacteroidales RF16 group and Anaerovibrio were the keystone genera in the CON and PEO groups, respectively, with fungal genera exclusively found in the PEO group but not identified as keystone taxa. Predicted function analysis using CowPI, CH4 metabolism was enriched in the CON group, whereas metabolism of sulfur (P < 0.001) and propionate (P < 0.1) were enriched in the PEO group. Random forest analysis identified eight ruminal metabolites, including propionate, that were altered after PEO administration, with predictive accuracy ranging from 0.75 to 0.88. Selenomonas was positively correlated with propionate and co-occurred with it.
Conclusions
The results provide an understanding of how PEO oral administration affects the ruminal microbial community and its functions in the rumen, as well as its linkages with rumen metabolites and host health, ultimately leading to the reduced CH4 emissions.
Pharmacokinetics and Bioequivalence of Two Formulations of Azithromycin Tablets: A Randomized, Single Dose, 3-Period Crossover Study in Healthy Chinese Volunteers Under Fasting and Fed Conditions
Authors: Chen Yingrong , Ye Libing , Mei Jue , Tian Mengli , Xu Min , Jin Qiuyue , Yu Xiang , Yang Shuixin , Wang Jie 
Published: September 2023

Abstract
Background
Azithromycin is the first azalidic antibiotic that is related to the macrolide family of antibiotics. This study was conducted to compare the bioequivalence of two azithromycin tablets under fasting and fed conditions in healthy Chinese volunteers.
Methods
The study was a single-dose, reference-replicated, 3-period crossover trial. A total of 72 healthy subjects (36 subjects in the fasting state study and 36 subjects in the fed study) were enrolled in the study and were randomized to treatment. After the administration of a single oral dose of a 250 mg generic azithromycin tablet (test) or branded azithromycin tablet (reference), blood samples were collected at specific time intervals from 0 to 120 hours. The plasma concentrations of azithromycin were measured by high-performance liquid chromatography–tandem mass spectrometry (HPLC‒MS/MS). Non-compartmental analysis method was employed to determine the pharmacokinetic parameters. Adverse events were also recorded.
Results
In fasting test, the either reference-scaled average bioequivalence (RSABE) approach was used to evaluate the bioequivalence of maximum plasma concentration (Cmax) (withinsubject standard deviation, SWR>0.294), while average bioequivalence (ABE) method was used to evaluate the bioequivalence of area under the cocentration-time curve from time 0 to the time of the last measurable plasma concentration (AUC0 − t ) and area under the cocentration-time curve from time 0 extrapolated to infinity (AUC0−∞) (SWR<0.294). The geometric mean ratio (GMR) of the test/reference for Cmax was 106.49%, and the 95% upper confidence bound was < 0. For AUC0 − t and AUC0−∞ comparisons, GMRs were 103.34% and 101.28%, and the 90% CIs of the test/reference were 95.90%-111.35%, 94.85%-108.15%, respectively. In fed test, the RSABE approach was used to evaluate the bioequivalence of Cmax (SWR>0.294), while ABE approach was used to evaluate the bioequivalence of AUC0 − t and AUC0−∞ (SWR<0.294). The GMRs for Cmax was 99.80%, while the 95% upper confidence bound value was < 0. For AUC0 − t and AUC0−∞ comparisons, GMRs were 97.07% and 98.15%, and the 90% CIs of the test/reference were 90.02%-104.68%, 90.66%-106.25%, respectively. All adverse events were mild and transient.
Conclusions
The trial demonstrated that the test and the reference azithromycin tablets were bioequivalent and well tolerated in Chinese volunteers under fasting and fed conditions.
Trial Registration:
Clinicaltrials, ChiCTR2300071630 (retrospectively registered in 19/05/2023).