bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis about the Continuous Participation Behavior of Dance for All Participants during the COVID-19 Pandemic by applying the Extended Health Belief Model
Authors: An Jung-Hun , Lim Sun-Young , Lee Ji-Seol 
Published: January 2023

Abstract
Background: Based on EHBM theory, this study added the concepts of DFAP’s attitudes toward COVID-19 and perceived behavior control to the main components perceived susceptibility, perceived benefit, and perceived barriers. In addition, these factors intended to verify the continuous behavioral intention of physical activity through DFA by empirically analyzing the influence relationship of the EHBM by CA into media information exposure and personal information exposure for COVID-19.
Methods:A survey was conducted on DFAP’s 665, who joined public sports facilities, academies, and clubs in Seoul and Gyeonggi Province in South Korea from August 2020 to February 2022. Data analysis was performed on Windows PC/SPSS 26.0 and AMOS 24.0 ver. frequency analysis, correlation analysis, confirmatory factor analysis and structural equation modeling were used to analyze the survey results.
Results:First, PB a sub-factor of the EHBM of have a statistically significant (p<0.05) effect on CPB. Second, the sub-factors of EHBM, CA and AT, have a statistically significant (p<0.001) effect on CPB. Third, PBC have a statistically significant (p<0.001) effect on CPB.
Conclusions:The results derived in the process of achieving this purpose are academically meaningful in that they present the direction of DFA activation in the 'New Normal Era' provide practical implications for related workers.
Transcription factor PRDM15 binds to the promoter mutation locus of PPP2R5C promoted proliferation of goat granulosa cells
Authors: Wang Peng , Li Wentao , Liu Ziyi , He Xiaoyun , Liu Yufang , Chu Mingxing 
Published: January 2023

Abstract
Protein phosphatase 2 regulatory subunit B’gamma (PPP2R5C) is involved in a variety of physiological processes in mammals, such as growth and development, cellular metabolism, and immunity. Here, the PCR sequencing and KASP genotyping sequencing were used to detect the distribution of SNPs of PPP2R5C, and then a series of molecular biology technology were used to detect the regulation pathway between PPP2R5C and kidding number trait of goats. The sequencing results showed that there was a g.65977460A > G mutation locus in the promoter region of PPP2R5C, and there were 223 individuals with genotype GG, 91 with genotype GA, and 51 with genotype AA in the tested population of Yunshang black goats. Association analysis showed that the kidding number trait of second and third parity were significantly higher for the individuals with AA genotype than those with the AG and GG genotypes. Subsequently, the results of dual luciferase activity assay showed that the promoter activity of PPP2R5C-mutant-A was significantly higher than that of PPP2R5C-Wild-G (P < 0.05). The transcription factor PRDM15 was predicted to bind to a mutation in PPP2R5C (g.65977460A > G). The results of ChIP-qPCR showed that the transcription factor PRDM15 could bind to the promoter region of PPP2R5C. Moreover, the expression of PPP2R5C, proliferation factors CDK4 and Cyclin-D2 in goat GCs was significantly increased after PRDM15 (PR/SET domain 15) overexpression (P < 0.05). While the results of EdU and CCK-8 showed that the proliferation rate of goat GCs increased after PRDM15 overexpression and the proliferation efficiency of goat GCs was significantly reduced after PRDM15 inhibition (P < 0.05).Finally, the expression of the PI3K-Akt pathway (phosphatidylinositol-3-kinase/protein kinase B) marker AKT2 (AKT serine/threonine kinase 2) was significantly increased in granulosa cells overexpressing PRDM15, and the opposite was true after inhibition of PRDM15. Together, our results showed that PPP2R5C g.65977460A > G was significantly associated with the kidding number in Yunshang black goats. The transcription factor PRDM15 was identified to bind to the promoter region of PPP2R5C g.65977460A > G mutation locus promoted the proliferation of goat granulosa cells (GCs) by the PPP2R5C/PI3K-Akt pathway. These results provide a new insight into the molecular mechanisms underlying the phenotypic changes in goats.
OsWRKY76 positively regulates drought stress via OsbHLH148-mediated jasmonate signaling in rice
Authors: Zhang Mingxing , Zhao Ranran , Huang Kai , Wei Zhiqi , Guo Boya , Huang Shuangzhan , Li Zhao , Jiang Wenzhu , Wu Tao , Du Xinglin 
Published: January 2023

Abstract
Drought stress is a major environmental threat that limits plant growth and crop productivity. Therefore, it is necessary to uncover the molecular mechanisms behind drought tolerance in crops. Here, OsWRKY76 positively regulated drought stress in rice. OsWRKY76 expression was induced by PEG treatment, dehydration stress, and exogenous MeJA rather than by no treatment. Notably, OsWRKY76 knockout weakened drought tolerance at the seedling stage and decreased MeJA sensitivity. OsJAZ12 was significantly induced by drought stress, and its expression was significantly higher in OsWRKY76-knockout mutants than in wild-type ZH11 under drought stress. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that OsWRKY76 interacted with OsJAZ12. OsWRKY76 weakened the interaction between OsbHLH148 and OsJAZ12 in yeast cells. The OsJAZ12 protein repressed the transactivation activity of OsbHLH148, and this repression was partly restored by OsWRKY76 in rice protoplasts. Moreover, OsDREB1E expression was lower in OsWRKY76-knockout mutants than in wild-type ZH11 under drought stress, but it was upregulated under normal growth conditions. Yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase assays showed that OsWRKY76 and OsbHLH148 bound directly to the OsDREB1E promoter and activated OsDREB1E expression in response to drought stress. These results suggest that OsWRKY76 confers drought tolerance through OsbHLH148-mediated jasmonate signaling in rice, offering a new clue to uncover the mechanisms behind drought tolerance.
The Vladimirov-Taibleson Operator: Inequalities, Dirichlet Problem, Boundary H\"older Regularity
Authors: Kochubei Anatoly 
Published: January 2023

Abstract
We study the Vladimirov-Taibleson operator, a model example of a pseudo-differential operator acting on real- or complex-valued functions defined on a non-Archimedean local field. We prove analogs of classical inequalities for fractional Laplacian, study the counterpart of the Dirichlet problem including the property of boundary H\"older regularity of solutions.
MSC 2020. Primary: 35S05. Secondary: 11S80, 47G30, 35S11; 35R11.
Appendicitis and peritonitis in children with a ventriculo-peritoneal shunt
Authors: Fröschle Glenn M C , Hagens Johanna , Mannweiler Philip , Groth Friederike Sophie , Kammler Gertrud , Reinshagen Konrad , Tomuschat Christian 
Published: January 2023

Abstract
Purpose:
The purpose of this study was to outline the management of patients with appendicitis and ventriculoperitoneal shunt (VPS).
Methods:
Patients with VPS presenting with an acute abdomen between 2012 and 2022 at a tertiary-care pediatric facility were the subject of a descriptive analysis. Patients were divided into two groups based on their diagnoses: group A (appendicitis) and group B (primary peritonitis). Medical records were analyzed to look at the diagnostics, operative approach, complications, peritoneal and liquor culture and antibiotic treatment.
Results:
A total of 17 patients were examined: 7 patients in group A and 10 individuals in group B. In the present study patients in group A typically present younger, sicker, and with more neurological symptoms than those in group B. All patients with appendicitis had their VPS exteriorized and a new shunt system into the peritoneum reimplanted 20 days later.
Conclusion
Surgery should be aggressively administered to patients who present with an acute abdomen and a VPS. In those with evident perforation, change of the whole shunt system is suggested. Shunt infection and dysfunction should be ruled out in patients with abdominal symptoms, and surgical care should be started with a low threshold.
High-tech equipment configuration and management policy assessment: An equity and efficiency analysis of MRI services in Henan province, China
Authors: Huang Xiaoling , Wei Yan , Sun Hui , Huang Jiaqi , Chen Yingyao , Cheng Jingliang 
Published: January 2023

Abstract
Background: By evaluating equity and effectiveness, this study provides evidence-based knowledge for scientific decision-making and the optimization of magnetic resonance imaging (MRI) configuration and utilization at the provincial level.
Methods: Using data of 2017, we apply a Gini coefficient to analyze the equity of MRI services in 11 sample cities in Henan province. An agglomeration degree is then applied to measure equity from the perspective of population and geography, and a data envelope analysis is used to evaluate MRI efficiency.
Results: The overall Gini coefficient of MRI allocation by population in the 11 sample cities is 0.117; however, the equity varies considerably among the sample cities. The sample’s comprehensive efficiency is only 0.732, indicating the overall ineffectiveness of provincial MRI utilization. The pure technical and scale efficiency of four sample cities are both below 1, indicating lower MRI effectiveness than the rest.
Conclusions: Although the overall equity of configuration at the provincial level is relatively good, equity varies at the municipal level. Our results show a low MRI utilization efficiency, which policymakers should consider carefully during policy implementation.
Evaluation of community-based vector surveillance system for routine entomological monitoring under low malaria vector densities and high bednet coverage in western Kenya
Authors: Abong’o Bernard , Stanton Michelle C. , Donnelly Martin J. , Ochomo Eric , Kuile Feiko O. ter , Samuels Aaron M. , Kariuki Simon , Musula George , Oxborough Richard , Munga Stephen , Torr Steve J. , Gimnig John E. 
Published: January 2023

Abstract
Introduction. Entomological surveillance is traditionally conducted by supervised teams of trained technicians. However, it is expensive and limiting in the number of sites visited. Surveillance through community-based collectors (CBC) may be more cost-effective and sustainable for longitudinal entomological monitoring. This study evaluated the efficiency of CBCs in monitoring mosquito densities compared to quality-assured sampling conducted by experienced entomology technicians.
Methodology. Entomological surveillance employing CBCs was conducted in eighteen clusters of villages in western Kenya using indoor and outdoor CDC light traps and indoor Prokopack aspiration. Sixty houses in each cluster were enrolled and sampled once every month. Collected mosquitoes were initially identified to the genus level by CBCs, preserved in 70% ethanol and transferred to the laboratory every two weeks. Parallel, collections by experienced entomology field technicians were conducted monthly by indoor and outdoor CDC light traps and indoor Prokopack aspiration and served as a quality assurance of the CBCs.
Results. Per collection, the CBCs collected 80% fewer An. gambiae s.l. [RR=0.2; (95%CI: 0.14 – 0.27)] and An. coustani [RR=0.2; (95%CI: 0.06-0.53)] and 90% fewer An. funestus [RR=0.1; (95%CI: 0.08-0.19)] by CDC light traps compared to the quality assured (QA) entomology teams. Significant positive correlations were however observed between the monthly collections by CBCs and QA teams for both An. gambiae and An. funestus. In paired identifications of pooled mosquitoes, the CBCs identified 4.3 times as many Anophelescompared to experienced technicians. The cost per person-night was lower in the community-based sampling at $9.1 compared to $89.3 by QA per collection effort.
Conclusion. Unsupervised community-based mosquito surveillance collected substantially fewer mosquitoes per trap-night compared to quality-assured collection by experienced field teams, while consistently overestimating the number of Anophelesmosquitoes during identification. However, the numbers collected were significantly correlated between the CBCs and the QA teams suggesting that trends observed by CBCs and QA teams were similar. Further studies are needed to evaluate whether adopting low-cost, devolved supervision with spot checks, coupled with remedial training of the CBCs, can improve community-based collections to be considered a cost-effective alternative to surveillance conducted by experienced entomological technicians.
Targeted-Gene Sequencing and Bioinformatics Analysis of Patients with Gallbladder Neuroendocrine Carcinoma: A Case Report and Literature Review
Authors: Yang Yunchuan , Chen Zhitao , Tang Hui , Wan Dalong , Liu Mulin 
Published: January 2023

Abstract
Background: Gallbladder Neuroendocrine carcinomas (NEC) is a subtype of gallbladder malignant tumour that is characterized by low incidence, high malignancy and poor prognosis. To date, the genetic alterations, mechanistic relationships among mutated genes and signaling pathways of gallbladder NEC are unclear.
Patient and Methods: This paper presents a case of rare primary gallbladder NECin a 73-year-old female patient. A radical cholecystectomy associated with hepatic hilar lymphadenectomy, resection of segments IV-B and V of the liver were performed. Targeted-gene sequencing and bioinformatics analysis tools, including STRING, GeneMANIA, Metascape, TRRUST, Sangerbox, cBioPortal and GSCA, are used to clarify the biological functions and features of mutated genes in gallbladder NEC.
Results: Twelve gene mutations (APC, ARID2, IFNA6, KEAP1, RB1, SMAD4, TP53, BTK, GATA1, GNAS and PRDM3) were observed, and a tumor mutation burden (TMB) of 9.52 muts/Mb was calculated in the gallbladder NEC using targeted-gene sequencing. Protein-protein interaction network was constructed, which showed the significant interactions among twelve mutated genes. In terms of the functions and pathways of twelve gene mutations based on GO and KEGG, 40 tumor-related results are presented in this paper. Key regulated factor of Neuroendocrine carcinoma of gallbladder-related genes was established. In addition, the biological functions and features of gallbladder NEC are further compared with gallbladder carcinoma.
Conclusion: Gallbladder NEC urgently requires standardized and effective treatment protocols. Gallbladder NEC can be compared with other gallbladder carcinoma that have characteristic clinical phenotype, molecular alterations, functional information and enrichment pathway.
To determine if changing to white light street lamps improves road safety: A multilevel longitudinal analysis of road traffic collisions during the relighting of Leeds, a UK city
Authors: Marchant Paul , Norman Paul 
Published: January 2023

Abstract
Background
Large-scale installation of broad-spectrum (white) road lighting has been claimed to bring about a substantial reduction in road traffic collisions (RTCs). This confirmatory study estimates the effect on personal injury RTCs of a relighting programme that installed nearly 80,000 new white lamps, between the years 2005 and 2013, throughout the large UK city of Leeds.
Methods
Time series of weekly RTC personal injury counts in 107 areas, within the city over nearly 9 years when its road lighting was almost completely relit, were analysed, using multilevel modelling. The background change in each area when and where no lighting was being changed was separated from that associated with when and where new replacement white lamps were installed by including a polynomial for the underlying time-trend. The key interest is how the installation of the new lamps affects the ratio of the rate of collisions occurring in darkness to those occurring in daylight. The measure sought is given by the daylight adjusted darkness collision rate ratio (CRR) for the specified amount of relighting, that is the factor by which the daylight adjusted collision rate changes by the specified change in lighting.
Results
The daylight adjusted darkness collision rate ratio (CRR) has a point estimate of 0.990 and a 95% confidence interval, CI (0.971, 1.010) for 100 replaced lamps; representing a range between a 3% reduction in the collision rate to a 1% increase. Using the series truncated at the end of 2011 that seems more trustworthy, gives a revised point estimate of 0.993 and a CI (0.971, 1.015). Both CIs include one, therefore no effect on road safety was detected. The CIs for any other number of lamps installed will also include one. Typically, the plausible range of road safety impact brought by the new white lamps for a typical area with 700 lamps, roughly spans 20% reduction to a 10% increase for the daylight adjusted darkness collision rate.
Conclusion
No convincing evidence was found for an improvement (or detriment) in road safety by relighting with white lamps, despite the extensive, city-wide installation efforts and associated costs.
Renoprotective Effect of Thymoquinone against Streptozotocin-Induced
Diabetic Nephropathy: Role of NOX2 and Nrf2 Signals
Authors: Hofni Amal , Ali Fares E.M. , Ibrahim Ahmed R. N. , Aboubaker Esam M. 
Published: January 2023

Objective:
Diabetic nephropathy is an unavoidable complication of chronic uncontrolled
diabetes mellitus. The pathogenesis of diabetic nephropathy is multifactorial, and the
development of an effective therapy remains to be elucidated. The aim of the present study was
to assess the role of NOX2 and Nrf2 in the protective mechanism of thymoquinone (THQ)
against streptozotocin (STZ)-induced diabetic nephropathy.

Methods:
Rats were injected with STZ (55 mg/kg) to induce diabetes. The diabetic rats were
orally treated with THQ (10 mg/kg/day) for eight weeks.

Results:
STZ-treated rats exhibit an elevation of serum creatinine, serum urea, and creatinine
clearance. The renal abnormalities were associated with increased NADPH oxidase isoform,
NOX2 protein expression, and activity, along with elevated malondialdehyde (MDA). In addition,
the tumor necrotic factor-alpha (TNF-α) level and nitric oxide (NO) bioavailability, as well as the
transforming growth factor-beta (TGF)-β, were markedly increased. On the other hand, the nuclear
factor-E2-related factor (Nrf2) protein expression was significantly reduced in diabetic rats
compared to the control. However, treatment with THQ significantly reversed these alterations
with subsequent ameliorating renal dysfunction and pathological abnormalities.

Conclusion:
The present study demonstrates that THQ could protect against STZ-induced diabetic
nephropathy by modulating the Nrf2/NOX2 signaling pathway.
Development of an Interpolation Scheme for Seismic Indexes in Lima Metropolitan, Peru
Authors: Garay Avendaño Roger Leonardo , Moya Huallpa Luis Angel , Gonzales Trujillo Carlos Eduardo , Lazares La Rosa Luis Fernando , Diaz Figueroa Miguel Augusto , Zavala Toledo Carlos Alberto , Yamazaki Fumio , Calderon Cahuana Diana Lucia , Huerta Gonzales Kevin Steve 
Published: January 2023

In recent years, the development of seismic networks in Metropolitan Lima, administrated by public and private institutions, has received special attention since it makes possible the quantification of different seismic indexes under the occurrence of earthquakes. Therefore, the integration of the information both from acceleration sensors and site conditions from microzoning studies allows the estimation of the possible extent of the damage in quasi-real time. In this study, the implementation of a system to evaluate seismic parameters in a uniform grid of 250 x 250 m2 resolution is reported. In this regard, peak ground acceleration (PGA) values from the available time-history records are computed and reduced to the engineering bedrock level. Then, by means of the interpolation technique called Ordinary Kriging, in which each seismic station is considered as a random variable and the correlation between a pair of such random variables depends only on the distance between their coordinates, the acceleration distribution is evaluated. Amplification factors are applied in order to finally bring the PGA up to the surface level. A quantitative evaluation of the accuracy of our results is performed using two recent earthquakes with moment magnitude larger than 5: the 2019 Mw 8.0 Lagunas earthquake and 2021 Mw 6.0 Mala earthquake. The results have reproduced to some extent the seismic response of the diverse geomorphological deposits in Metropolitan Lima and suggest the inclusion of a larger number of strong motion stations in order to reduce the estimation errors.
Designing and Developing an eHealth Program for Patients With Persistent Physical Symptoms: Usability Study
Authors: Christensen Oliver Rønn , Hedegaard Leonora , Rask Mette Trøllund , Clemensen Jane , Frostholm Lisbeth , Rosendal Marianne 
Published: January 2023

Background
Patients with persistent physical symptoms presenting in primary care are often affected by multiple symptoms and reduced functioning. The medical and societal costs of these patients are high, and there is a need for new interventions tailored to both the patients and health care system.

Objective
This study aimed to examine the usability of an unguided, self-help treatment program, “My Symptoms,” developed to assist patients and general practitioners in symptom management.

Methods
In all, 11 users (4 patients with persistent physical symptoms and 7 laypeople) participated in web-based thinking-aloud interviews involving the performance of predefined tasks in the program. Thematic analysis was used to categorize the severity of usability issues. General usability heuristics were cross-referenced with the usability issues.

Results
The analysis identified important usability issues related to functionality, navigation, and content. The study shows how therapeutic knowledge in some cases was lost in the translation of face-to-face therapy to a digital format. The user testing helped uncover how the functionality of the digital elements and general navigation of the program played a huge part in locating and accessing the needed treatment. Examples of redesign to mediate the therapeutic value in the digital format involving health care professionals, web developers, and users are provided. The study also highlights the differences of involving patients and laypeople in the interviews.

Conclusions
Taking the experience of common symptoms as a point of departure, patients and laypeople contributed to finding usability issues on program functionality, navigation, and content to improve the program and make the treatment more accessible to users.