bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal relationship between asthma and inflammatory bowel disease: A two-sample bidirectional mendelian randomization analysis
Authors: Lai Jianxiong , Fang Bin , Luo Lirong , Xie Wenjie , Fu Yi , Li Jian 
Published: March 2023

AbstractBackground Based on the findings of current observational studies, asthma and inflammatory bowel disease (including Crohn's disease and ulcerative colitis) are associated; however, their causal association cannot be established due to methodological limitations. Therefore, we use two-sample bidirectional mendelian randomization (MR) to overcome the confounding factors and explore the causal link between the two diseases. Methods After selecting asthma and IBD-related genome-wide association studies (GWAS) data and screening single nucleotide polymorphisms (SNPs), MR analysis was performed by four methods: inverse variance weighted (IVW), MR-Egger, maximum likelihood, and weighted median (WM), while Cochran's Q test was used to detect heterogeneity and MR-Egger intercept to detect horizontal pleiotropy. Finally, we used the leave-one-out method and funnel plot to perform sensitivity analysis. Results We screened 57, 59, and 60 SNPs in the association analysis of asthma and IBD, CD, and UC, respectively. The results of MR analysis showed that asthma only increased the risk of CD (IVW: OR = 1.1712, 95% CI = 1.0418–1.3167, P value = 0.0082; maximum likelihood: OR = 1.1739, 95% CI = 1.0428–1.3215, P value = 0.0080). In reverse MR analysis, there may be suggestive positive results for IBD and asthma despite not meeting the Bonferroni correction (IVW: OR = 1.0165, 95% CI = 1.0023–1.0333, P value = 0.0222; maximum likelihood: OR = 1.0168, 95% CI = 1.0025–1.0314, P value = 0.0212). Neither forward nor reverse MR analysis revealed heterogeneity or horizontal pleiotropy. Similarly, we did not find potential directional pleiotropy by funnel plot, and the leave-one-out method did not suggest a significant effect of a single SNP on the overall results. Conclusions Our study found that asthmatic patients had a higher risk of CD, suggesting that there may be a common pathophysiological process between asthma and CD.
A rat model of functional free muscle transfer for facial reanimation and establishment of a thoracodorsal artery-based muscle flap
Authors: Kim Jong-Ho , Park Sunkyu , Lee Seung Jun , Heo Chanyoung 
Published: March 2023

Abstract
Introduction

Facial paralysis is a debilitating condition that can have a significant impact on a person's quality of life. Functional free muscle transfer (FFMT) is a surgical technique that has been used to improve facial paralysis, but there are limited studies on the anatomical approach and use of an animal model. The purpose of this study was to establish a rat model of FFMT in facial paralysis.

Methods

In this study, a total of twelve Wister rats weighing over 250 g were used to establish a rat model for FFMT using the ipsilateral motor nerve. All procedures were conducted under general anesthesia, and a preauricular incision with a mandibular extension was made to expose the buccal and marginal branches of the facial nerve. An axillary incision was also made to identify and dissect the thoracodorsal vessel and nerve. A partial latissimus dorsi muscle was then transferred to the facial region and the facial vessels and mandibular branch of the facial nerve were anastomosed to the thoracodorsal vessels and thoracodorsal nerve, respectively.

Results

Our study successfully established a functional muscle flap based on the thoracodorsal vessel in a rat model. A total of 10 rats underwent the surgical procedure, and nine survived the postoperative period. Four months after surgery, the transferred muscle flaps were assessed, and six out of nine were viable. Among the viable flaps, five showed successful innervation.

Conclusion

This study has successfully established a rat model of FFMT surgery, which is a commonly performed procedure for treating facial paralysis. This model has the potential to be used in various experimental applications aimed at improving surgical outcomes, not only for facial paralysis but also for other muscle transfer procedures.
Understanding consumers' intention to use autonomous delivery vehicles during the COVID-19 pandemic: The stimulus-organism-response approach
Authors: chunhua Ju , zhirong HuHu , Shuo Wang 
Published: March 2023

Abstract
Autonomous delivery vehicles (ADVs) have attracted much attention since COVID-19. As an emerging last-mile delivery tool, the large-scale implementation of ADVs depends on consumers’ willingness to adopt them. However, until recently, research on user acceptance and adopting emerging technology has been relatively rare. The present study is the first that applies the stimulus-organism-response (S-O-R) model to investigate how stimulating factors (COVID-19 risk and human-computer interaction) affect consumers’ intention to use ADVs in last-mile delivery by triggering consumers’ inner states (delivery risk, price sensitivity, perceived enjoyment, trust in technology). Quantitative data based on university students in Zhejiang, China, was collected through an online survey platform (n = 298), and structural equation modeling was undertaken. The results reveal that COVID-19 risk and human-computer interaction can lead to adoption behaviors by triggering different inner states of consumers. Further, innovation is also considered to be one of the factors determining behavioral intention. The findings have profound theoretical and practical contributions to last-mile delivery and technology acceptance research.
The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response
Authors: Schwab Melissa , Dezfouli Ali Bashiri , Khosravi Mohammad , Alkotub Bayan , Birgani Mohammad Javad Tahmasebi , Multhoff Gabriele 
Published: March 2023

Abstract
Background
An enhanced aerobic glycolysis (Warburg effect) associated with an increase in lactic acid in the tumor microenvironment contributes to tumor aggressiveness and resistance to radiation and chemotherapy. We investigated the radiation- and chemo-sensitizing effects of the nonsteroidal anti-inflammatory drug (NSAID) diclofenac in different cancer cell types.
Methods
The effects of a non-lethal concentration of diclofenac was investigated on the MYC expression, lactate dehydrogenase (LDH) activity and heat shock protein response in human colorectal (LS174T, LoVo), lung (A549), breast (MDA-MB-231) and adeno pancreatic (COLO357) carcinoma cells. Radiation- and chemo-sensitization of diclofenac was determined in clonogenic cell survival assays and in a xenograft tumor mouse model.
Results
We demonstrated that a non-lethal concentration of diclofenac affects the LDH activity, decreases MYC expression, reduces cytosolic Heat shock factor 1 (HSF1), Hsp70 and Hsp27 levels and membrane Hsp70 positivity in colorectal LS174T and LoVo cancer cells but not in lung A549 carcinoma cells, MDA-MB-231 breast cancer cells and COLO357 pancreatic adenocarcinoma cells. The impaired lactate metabolism and stress response in diclofenac-sensitive colorectal cancer cell lines was associated with a significant increase in the sensitivity towards radiation and 5fluorouracil (5-FU), in vitro, and in a xenograft colon carcinoma mouse model diclofenac induces radio-sensitization, in vivo.
Conclusion
Our results suggest that a decrease in the LDH activity and/or stress response upon diclofenac treatment predicts its radiation/chemo-sensitizing capacity.
Dual chimeric antigen receptor T cells targeting CD38 and SLAMF7 with independent signalling demonstrate efficacy and safety in preclinical models of Multiple Myeloma
Authors: Roders Nathalie , Nakid-Cordero Cecilia , Raineri Fabio , Fayon Maxime , Abecassis Audrey , Choisy Caroline , Nelson Elisabeth , Maillard Claire , GARRICK David , Talbot Alexis , Fermand Jean-Paul , Arnulf Bertrand , BORIES JEAN-CHRISTOPHE 
Published: March 2023

Abstract
Chimeric antigen receptor T-cell (CAR-T) therapy for multiple myeloma (MM) targeting B-cell maturation antigen (BCMA) induces high overall response rates. However, relapse still occurs and novel strategies for targeting MM cells by CAR-T are needed. SLAMF7 (CS1) and CD38 on tumour plasma cells represent potential alternative targets for CAR-T in MM, but their expression on activated T cells and other hematopoietic cells raises concerns about the efficacy and safety of such treatments. Here, we used CRISPR/Cas9 deletion of the CD38 gene in T cells and developed DCAR, a double CAR system targeting CD38 and CS1 through activation and co-stimulation receptors, respectively. We show that inactivation of CD38enhances the anti-MM activity of DCAR-T in vitro. Edited DCAR-T showed strong in vitro and in vivo responses specifically against target cells expressing both the CD38 and the CS1 antigens. Importantly, we provide evidence that, unlike anti-CD38 CAR-T, which elicited a rapid immune reaction against hematopoietic cells in a humanized mouse model, DCAR-T showed no signs of toxicity. Thus, DCAR-T could provide a safe and efficient alternative to treat MM patients.
Scalable Detector Design for a High-Resolution Fast-Neutron Radiography Panel
Authors: Young Christian , Browning Chloe , Thurber Ryan , Smalley Matthew , Liesenfelt Michael , Hayward Jason , McFarlane Nicole , Preston Jeff 
Published: March 2023

Abstract
Development of a scalable flat-panel neutron radiography device is needed to meet the nondestructive testing needs of a growing industrial market. Flood field images from a Cs-137 gamma source and a Cf-252 fission neutron source were generated using a detector composed of a 3-mm-thick sheet of EJ-200, and 3-mm-thick sheet of acrylic light spreader, an 8 × 8 array of SensL MICROFJ-60035-TSV SiPMs, and an IDEAS ROSSPAD readout module. The readout module can be tiled together with similar systems to create a panel of any size, and is only limited by the number of available Ethernet ports in a switching system. A collimated gamma line image demonstrated a 90% spatial resolution of approximately 0.47 line pairs per centimeter and a 10% spatial resolution of approximately 2.32 line pairs per centimeter. A neutron edge image demonstrated a 90% spatial resolution of approximately 0.70 line pairs per centimeter and a 10% spatial resolution of approximately 3.35 line pairs per centimeter. Both of these images show the ability to generate radiographs with sub-SiPM spatial resolution. Using these readout modules, a large-scale radiographic panel can be developed by tiling ROSSPAD modules together.
Curcumin suppresses melanoma cell migration through down-regulating NEDD4 and inhibiting the endocytic degradation of E-cadherin
Authors: Wo xiaoman , Zhang Zepeng , Cai Limin , Dong Yanyan , Qin Wenxin , Liu Tian , Ma Yanli , Wang Yu , Liu Jing , Zhao Wenran , Wang Yongchen 
Published: March 2023

Abstract
Objective Melanoma is one of the most aggressive cancers which tends to metastasize beyond the primary site. To block or delay cancer metastasis is critical for melanoma treatment. Curcumin exerts inhibiting the proliferation and metastasis of melanoma, while its anti-metastasis mechanism is not completely understood.
Methods The melanoma cell lines A375 and A875 were used in this experiment. Cell migration was determined by Transwell assay with curcumin. The expression of Neuronal precursor cell-expressed developmentally downregulated 4 (NEDD4), E-cadherin, and vimentin was determined by Western blotting and/or quantitative real-time PCR in the context of NEDD4 knockdown or overexpression or curcumin treatment. The endocytosis of E-cadherin was observed by fluorescence microscopy in the cells with NEDD4 (wild type or mutated) overexpression or curcumin treatment.
Results We demonstrated that curcumin inhibited the migration of melanoma cells through upregulating E-cadherin while inhibiting vimentin. The inhibited epithelial-mesenchymal transition (EMT) of the melanoma cells treated with curcumin was associated with the decreased expression of NEDD4. Knockdown of NEDD4 upregulated E-cadherin, while overexpression of NEDD4 depleted E-cadherin. Moreover, NEDD4 promoted the internalization and lysosomal degradation of E-cadherin, while curcumin strengthened the localization of E-cadherin in the plasma membrane and inhibited the delivery of E-cadherin to endosome. NEDD4 colocalized with the internalized E-cadherin, while mutated NEDD4, which still enhanced E-cadherin internalization, did not colocalize with E-cadherin.
Conclusions Taken together, curcumin’s inhibition on NEDD4 expression and E-cadherin endocytosis contributes to its anti-migration effect on melanoma cells.
The Mirror of the future: Building an InteractiveSmart Mirror with AI-based Virtual Assistant and Intruder Alert (Theft Detection)
Authors: N Nalini , Shetty Ashish , CM Abhishek 
Published: March 2023

Abstract
The smart mirror is a novel interactive device that integrates digital information and media content into a reflective surface. Unlike traditional mirrors, the smart mirror can display personalized information and real-time data such as weather, news, calendar events, and traffic updates, while still functioning as a regular mirror. The mirror uses a combination of voice control and a touch interface to enable users to interact with the display. Additionally, the smart mirror incorporates facial recognition technology to recognize the user and tailor content to their specific preferences. The mirror also has a built- in camera and PIR sensor that can be used to detect intruders. The smart mirror has a sleek, minimalist design that can fit in any modern home or office, and it offers a unique and innovative way to stay connected and informed while getting ready in the morning or throughout the day.
Comparative evaluation of unintentional extrusion of gutta-percha versus sealer on teeth presenting periapical radiolucency: a retrospective radiographic study on treatment outcome
Authors: Krikeli Eleni , Lambrianidis Theodoros , Molyvdas Ioannis , Mikrogeorgis Georgios 
Published: March 2023

AbstractObjective:The purpose of this study is the radiographic evaluation of endodontically treated teeth presenting periapical radiolucency and unintentional overfilling with gutta-percha or sealer on treatment outcome and persistence of the extruded materials.Materials and Methods: After assessment using periapical index (PAI), 202 roots filled with gutta-percha and Roth 811 sealer, exhibiting unintentional overfilling and periapical radiolucency were selected. All cases had at least 1 year follow-up. Type of extruded material, periapical status, and removal/persistence of the extruded material were evaluated. Data were statistically analyzed using logistic and linear regression analysis.Results:Tooth location, follow-up period and type of the extruded material were the only factors that significantly affected the outcome (p<0.05). The persistence of extruded material was significantly affected by its type and the recall time (p<0.05).Conclusions:It can be concluded that posterior roots, overfilling with sealer compared to gutta-percha and cases with long recall period present a significantly better rate of complete healing.
Patterned non-determinism in communication complexity
Authors: Gavinsky Dmytro 
Published: March 2023

Abstract
We define and study the model of patterned non-determinism in bipartite communication complexity, denoted by PNPX↔Y. It generalises the known models UPX↔Y and FewPX↔Y through relaxing the constraints on the witnessing structure of the underlying NPX↔Y -protocol.
It is shown that for the case of total functions PNPX↔Y equals PX↔Y (similarly to UPX↔Y and FewPX↔Y). Moreover, the corresponding exhaustive witness-searching problem – determining the full set of witnesses that lead to the acceptance of a given input pair – also has an efficient deterministic protocol.
Structurally, the possibility of efficient exhaustive PNPX↔Y -search summarises the above results and can be stated like this: if f1, . . . . ,fm are bipartite total Boolean functions with efficient deterministic protocols, then for every input (x, y) the set {i|fi (x, y) = ⊤} can be found by a deterministic protocol of cost poly-logarithmic in n and the total number of such sets for these fi’s.
Finally, the possibility of efficient exhaustive PNPX↔Y -search is used to analyse certain three-party communication regime (under the “number in hand” input partition): The corresponding three-party model is shown to be as strong qualitatively as the weakest among its two-party amplifications obtained by allowing free communication between a pair of players.
Experiences of cystic fibrosis newborn screening and genetic counseling
Authors: Foil Kimberly , Christon Lillian , Kerrigan Cheryl , Flume Patrick A. , Drinkwater Jaclyn , Szentpetery Sylvia 
Published: March 2023

Abstract
The South Carolina cystic fibrosis (CF) newborn screening (NBS) program changed in 2019 to include CFTR genotyping for babies with top 4% immunoreactive trypsinogen, which improves sensitivity and timeliness, but increases carrier detection. Carrier identification has genetic implications for the family and parents of NBS+ babies have increased emotional distress. Genetic counseling (GC) may increase parent understanding and reduce anxiety yet is not uniformly offered at CF centers. We report our early results after implementing GC for NBS+ families at the time of sweat chloride testing (SCT). Sixteen mothers participated in an online survey about their experience. The child’s pediatrician notified 10 (62.5%) of the NBS+ result. Parents felt they were notified in a timely manner (68.8%), by someone knowledgeable about NBS (62.5%), SCT (62.5%), CF (43.8%), and genetics (43.8%) and who cared about them (81.3%). Parents felt worried (81.3%), confused (81.3%), empowered (25%) and other (sad, shocked, scared, overwhelmed, devastated, defeated). Most (87.5%) sought additional information before SCT. Sadness and anxiety increased transiently with NBS+ result; bondedness with the baby was not affected. Nine mothers received GC with suggestion of reduced distress. Knowledge was high among both cohorts. GC was rated very helpful, informative, comforting, and minimally distracting. Data from this single-center study suggest benefit of GC and that families would value earlier contact with an expert.
Differential Cognitive and Behavioral Development from 6 to 24 Months in Autism and Fragile X Syndrome
Authors: Mullin Lindsay J. , Rutsohn Joshua , Gross Julia L. , Caravella Kelly E. , Weisenfeld Leigh Anne , Flake Lisa , Botteron Kelly N. , Dager Stephen R. , Estes Annette M. , Pandey Juhi , Schultz Robert T. , John Tanya St. , Wolff Jason J. , Shen Mark D. , Piven Joseph , Hazlett Heather C. , Girault Jessica B. 
Published: March 2023

Abstract
Background: Specifying early developmental differences among neurodevelopmental disorders with distinct etiologies but similar clinical phenotypes is critical to improving early identification and tailored intervention during the first years of life. Recent studies have uncovered important differences between infants with fragile X syndrome (FXS) and infants with familial history (FH) of autism spectrum disorder (ASD) who go on to develop autism themselves (FH-ASD), including differences in brain development and behavioral symptomology. However, there have been no studies investigating differential developmental skill profiles in FXS and FH-ASD infants.
Methods: The current study contrasted longitudinal trajectories of verbal (expressive and receptive language) and nonverbal (gross and fine motor, visual reception) skills in infants with FXS and FH-ASD infants, compared to typically developing controls and FH infants who did not develop ASD (FH-nonASD).
Results: Infants with FXS showed delays in developmental composite scores compared to FH-ASD (and FH-nonASD and control) infants as early as 6 months of age. At 12 months an ordinal pattern was established between groups on all domains tested, such that controls > FH-nonASD > FH-ASD > FXS. This pattern persisted through 24 months.
Conclusions: Our results demonstrate detectable delays by 6 months in FXS, whereas FH-ASD infants were indistinguishable from typically developing infants until 12 months. This highlights an earlier onset of global cognitive delays in FXS and, conversely, a protracted period of more subtly emerging delays in FH-ASD. Divergent neural and cognitive development in infancy between FXS and FH-ASD add to our understanding of important distinctions in the development and behavioral phenotype of these two groups.
Adipose tissue IL-18 production is independent of caspase-1 and caspase-11
Authors: Román-Domínguez Luis , Salazar-León Jonathan , Pérez-Martínez Leonor , Pedraza-Alva Gustavo 
Published: March 2023

Abstract

Inflammation in the adipose tissue resulting from imbalanced caloric intake and energy expenditures promotes the metabolic alteration observed in obesity. This inflammatory process is initiated by the production of inflammatory cytokines like IL-1b and IL-18. Opposing functions for these cytokines on the metabolism have been documented. While IL-1b promotes insulin resistance and diabetes, IL-18 promotes energy expenditures and regulates food intake. The production of IL-1b and IL-18 in the adipose tissue of obese mice was thought to be mediated by caspase-1 upon activation of the Nlrp3 inflammasome in response to lipid excess, and by the Nlrp1b inflammasome activation in response to energy excess, respectively. However, this is not the case, here we show that IL-18 production in the adipose tissue is independent of caspase-1 activity. Together, our results indicate that in response to metabolic stress signals IL-18 production is mediated by the Nlrp1b inflammasome in a caspase-1-independent manner while the Nlrp3-mediated IL-1b production is caspase-1 dependent.
Evaluation of the Breathing Mode by Infrared Thermography
Authors: Telson Yasmim Carvalho , Furlan Renata Maria Moreira Moraes , Porto Matheus Pereira , Ferreira Rafael Augusto Magalhães , Motta Andréa Rodrigues 
Published: March 2023

Abstract

Objective
To analyze breathing modes with infrared thermography.
Methods
Cross-sectional observational exploratory study conducted in 20 female participants with a mean age of 26.0 years. The thermograms were made following the principles of the American Academy of Thermology and the Brazilian Thermology Society. The camera FLIR A315 (FLIR Inc., Santa Barbara, CA) was used for the tests. The recordings consisted of the participants breathing normally through the nose for 2 minutes and simulating oral/oronasal breathing for another 2 minutes. The thermograms were analyzed with the FLIR Tools software. An ellipse was placed between the nostrils and the lip commissures to obtain the mean temperatures. The collection was made by two independent researchers, and the normalized non-dimensional temperature was calculated.
Results
The temperature in nasal breathing is higher than in oral/oronasal breathing both for inhaling and exhaling when measured in the region of the mouth. The exhaling temperatures were higher than the inhaling ones in oral/oronasal breathing (through the nose and the mouth) and nasal breathing (only through the nose). The temperature difference between exhaling and inhaling (ΔT) was greater in oral/oronasal breathing when measured in the region of the mouth.
Conclusion
The thermographic assessment of breathing modes may be made by comparing the mean temperatures of the mouth, using an ellipse.