bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection for endogenous retroviral loci in Jungle fowl using whole-genome sequencing
Authors: Ishihara Shinya 
Published: January 2023

AbstractChickens harbor two primary families of endogenous retroviruses (ERVs): the avian leukosis virus (ALV) and endogenous avian retrovirus (EAV) families. Characterization of ERVs can provide crucial insights into avian evolution. This study aimed to identify novel ERV loci absent in the reference genome using whole-genome sequencing data of red junglefowl, gray junglefowl, Ceylon junglefowl, and green junglefowl. A total of 837 ERV loci were identified across the four Gallus species. The number of ERV loci detected in red junglefowl with its subspecies, gray junglefowl, Ceylon junglefowl, and green junglefowl, was 363, 217, 194, and 129 loci, respectively. The phylogenetic tree was congruent with previously reported trees, suggesting the potential for inferring relationships among past junglefowl populations from the identified ERV loci. Of the detected loci, 306 ERVs were identified near or within the genes, and some were associated with cell adhesion. The detected ERV sequences was in the long terminal repeat region and were classified as EAV-family, ALV-E, Ovex-1, and murine leukemia virus related ERVs. In addition, the sequence of the EAV family was divided into four patterns by combining the U3, R, and U5 regions. These findings contribute to a more comprehensive understanding of the characteristics of chicken ERVs.
Network-based cytokine inference implicates Oncostatin M as a driver for inflammation phenotype of knee osteoarthritis
Authors: Iijima Hirotaka , Zhang Fan , Ambrosio Fabrisia , Matsui Yusuke 
Published: January 2023

SUMMARYInflammatory cytokines released by the synovium after trauma disturb the gene regulatory network and have been implicated in the pathophysiology of osteoarthritis. A mechanistic understanding of how aging perturbs this process can help identify novel interventions. Here, we introduced network paradigms to simulate cytokine-mediated pathological communication between the synovium and cartilage. Cartilage-specific network analysis of injured young and aged murine knees revealed aberrant matrix remodeling as a transcriptomic response unique to aged knees displaying accelerated cartilage degradation. Next, network-based cytokine inference with pharmacological manipulation uncovered IL6 family member, Oncostatin M, as a driver for the aberrant matrix remodeling. By implementing a phenotypic drug discovery approach, we identified that the activation of Oncostatin M recapitulated “inflammation” phenotype of knee osteoarthritis and highlighted high-value targets for drug development and repurposing. These findings offer translational opportunities targeting the inflammation-driven osteoarthritis phenotype.GRAPHICAL ABSTRACT
Group BStreptococcusdrives major transcriptomic changes in the colonic epithelium
Authors: Domínguez Kristen , Lindon April K. , Gibbons Justin , Darch Sophie E. , Randis Tara M. 
Published: January 2023

AbstractGroup B Streptococcus (GBS) is a leading cause of infant sepsis worldwide. Colonization of the gastrointestinal tract is a critical precursor to late-onset disease in exposed newborns. Neonatal susceptibility to GBS intestinal translocation stems from intestinal immaturity; however, the mechanisms by which GBS exploits the immature host remain unclear. β-hemolysin/cytolysin (βH/C) is a highly conserved toxin produced by GBS capable of disrupting epithelial barriers. However, its role in the pathogenesis of late-onset GBS disease is unknown. Our aim was to determine the contribution of βH/C to intestinal colonization and translocation to extraintestinal tissues. Using our established mouse model of late-onset GBS disease, we exposed animals to GBS COH-1 (WT), a βH/C-deficient mutant (KO), or vehicle control (PBS) via gavage. Blood, spleen, brain, and intestines were harvested 4 days post-exposure for determination of bacterial burden and isolation of intestinal epithelial cells. RNA-sequencing was used to examine the transcriptomes of host cells followed by gene ontology enrichment and KEGG pathway analysis. A separate cohort of animals was followed longitudinally to compare colonization kinetics and mortality between WT and KO groups. We demonstrate that dissemination to extraintestinal tissues occurred only in the WT exposed animals. We observed major transcriptomic changes in the colons of colonized animals, but not in the small intestines. We noted differential expression of genes that indicated the role of βH/C in altering epithelial barrier structure and immune response signaling. Overall, our results demonstrate an important role of βH/C in the pathogenesis of late-onset GBS disease.
A new mouse model of ATR-X syndrome carrying a common patient mutation exhibits neurological and morphological defects
Authors: Tillotson Rebekah , Yan Keqin , Ruston Julie , de Young Taylor , Córdova Alex , Cardin Valérie Turcotte- , Yee Yohan , Taylor Christine , Visuvanathan Shagana , Babbs Christian , Ivakine Evgueni A , Sled John G , Nieman Brian J , Picketts David J , Justice Monica J 
Published: January 2023

AbstractATRX is a chromatin remodelling ATPase that is involved in transcriptional regulation, DNA damage repair and heterochromatin maintenance. It has been widely studied for its role in ALT-positive cancers, but its role in neurological function remains elusive. Hypomorphic mutations in the X-linked ATRX gene cause a rare form of intellectual disability combined with alpha-thalassemia called ATR-X syndrome in hemizygous males. Patients also have facial dysmorphism, microcephaly, musculoskeletal defects and genital abnormalities. Since complete deletion of ATRX in mice results in early embryonic lethality, the field has largely relied on conditional knockout models to assess the role of ATRX in multiple tissues. Given that null alleles are not found in patients, a more patient-relevant model was needed. Here, we have produced and characterised the first patient mutation knock-in model of ATR-X syndrome, carrying the most common patient mutation, R246C. This is one of a cluster of missense mutations located in the chromatin interaction domain that disrupts its function. The knock-in mice recapitulate several aspects of the patient disorder, including craniofacial defects, microcephaly and impaired neurological function. They provide a powerful model for understanding the molecular mechanisms underlying ATR-X syndrome and for testing potential therapeutic strategies.
ADAR1p150 Prevents MDA5 and PKR Activation via Distinct Mechanisms to Avert Fatal Autoinflammation
Authors: Hu Shi-Bin , Heraud-Farlow Jacki , Sun Tao , Liang Zhen , Goradia Ankita , Taylor Scott , Walkley Carl R , Li Jin Billy 
Published: January 2023

SummaryEffective immunity requires the innate immune system to distinguish foreign (non-self) nucleic acids from cellular (self) nucleic acids. Cellular double-stranded RNAs (dsRNAs) are edited by the RNA editing enzyme ADAR1 to prevent their dsRNA structure pattern being recognized as viral dsRNA by cytoplasmic dsRNA sensors including MDA5, PKR and ZBP1. A loss of ADAR1-mediated RNA editing of cellular dsRNA activates MDA5. However, additional RNA editing-independent functions of ADAR1 have been proposed, but a specific mechanism has not been delineated. We now demonstrate that the loss of ADAR1-mediated RNA editing specifically activates MDA5, while loss of the cytoplasmic ADAR1p150 isoform or its dsRNA binding activity enabled PKR activation. Deleting both MDA5 and PKR resulted in complete rescue of the embryonic lethality ofAdar1p150-/-mice to adulthood, contrasting with the limited or no rescue by removing MDA5, PKR or ZBP1 alone, demonstrating that this is a species conserved function of ADAR1p150. Our findings demonstrate that MDA5 and PKR are the primaryin vivoeffectors of fatal autoinflammation following the loss of ADAR1p150.
Personal values and travel social media use among Generation Z
Authors: Chang Tung-Zong (Donald) , Kong Weng Hang , Bahl Angelica 
Published: January 2023

Purpose
The purpose of this study is to examine how one's social media use during travel, to connect and/or to update, is related to selected personal values among Generation Z. Hypotheses are proposed based on how values are related to two common dimensions: openness-to-change and self-enhancement.

Design/methodology/approach
A survey was conducted using existing and self-developed scales with 177 college students, a group of Chinese Gen Zers, at a public higher education institution in Macao, China.

Findings
Results show that community values and materialistic values have a significant influence on social media use, whereas family values do not. Between two distinctive social media uses, consumers with a stronger materialistic value are more likely to use social media to update. Subjects with a stronger community value are more likely to use social media to connect.

Originality/value
The study scrutinizes how mobile social media may be used in travel among Generation Z, who grew up with mobile devices and social media while forming personal values under the influence of families, communities and external environments. Travel destinations and services could streamline social media marketing efforts at those personal values that are relevant to their target markets. The results offer practical applications and directions for future research.
Relationship between digital twin and building information modeling: a systematic review and future directions
Authors: Radzi Afiqah R. , Azmi Nur Farhana , Kamaruzzaman Syahrul Nizam , Rahman Rahimi A. , Papadonikolaki Eleni 
Published: January 2023

PurposeDigital twin (DT) and building information modeling (BIM) are interconnected in some ways. However, there has been some misconception about how DT differs from BIM. As a result, industry professionals reject DT even in BIM-based construction projects due to reluctance to innovate. Furthermore, researchers have repeatedly developed tools and techniques with the same goals using DT and BIM to assist practitioners in construction projects. Therefore, this study aims to assist industry professionals and researchers in understanding the relationship between DT and BIM and synthesize existing works on DT and BIM.Design/methodology/approachA systematic review was conducted on published articles related to DT and BIM. A total record of 54 journal articles were identified and analyzed.FindingsThe analysis of the selected journal articles revealed four types of relationships between DT and BIM: BIM is a subset of DT, DT is a subset of BIM, BIM is DT, and no relationship between BIM and DT. The existing research on DT and BIM in construction projects targets improvements in five areas: planning, design, construction, operations and maintenance, and decommissioning. In addition, several areas have emerged, such as developing geo-referencing approaches for infrastructure projects, applying the proposed methodology to other construction geometries and creating 3D visualization using color schemes.Originality/valueThis study contributed to the existing body of knowledge by overviewing existing research related to DT and BIM in construction projects. Also, it reveals research gaps in the body of knowledge to point out directions for future research.
Emerging technologies for digitalized learning in higher education
Authors: Janahi Yusuf Mohamed , AlDhaen Esra , Hamdan Allam , Nureldeen Waleed Ahmed 
Published: January 2023

Purpose
Academic institutions, for the most part, discontinued face-to-face classes in favor of adopting and deploying online learning modalities that allowed for immediate participation. The pandemic has hastened the pace of implementation as well as the utilization of and reliance on technology. Artificial intelligence (AI) is important for higher education business continuity. Currently, some institutions are utilizing these resources to strengthen their student recruitment and retention efforts. Others use them to make the classroom more accessible or to construct tailored learning programs.

Design/methodology/approach
The rapid spread of the deadly COVID-19 pandemic in early 2020 has compelled many countries to enact stringent measures to halt the virus’s spread. The pandemic has hastened the adoption of online teaching and remote work technology. While a combination of online and face-to-face learning is the way of the future, it will necessitate additional resources to support program development and delivery, as well as increased collaboration between IT and subject matter experts.

Findings
This successful technological integration, which includes a smooth transition from face-to-face training to digital e-courses, provides a variety of benefits, including money saved on travel expenses. Top technological developments will continue to enhance company innovation and efficiency while also improving service efficiency. The top strategic technology trends for this year fall into three categories: human centricity, location independence, and resilient delivery, and are expected to be significant for the next five to ten years. Higher Education Institutions (HEIs) will need to establish a technological ecosystem that is dependable, cloud-based, data-integrated, and learning-focused to compete successfully in this “new normal.” After the epidemic, when classes resume on campus, a hybrid approach to virtual learning is likely to become the new normal. While it is unlikely that campuses would be totally virtual, they will also be unlikely to be entirely physical.

Originality/value
A blend of actual and virtual classrooms, as well as online learning, is the long-term solution, and strategic decisions made now will be critical in preparing for a post-pandemic world.
Green management practices and trust for green behavioral intentions and mediation of ethical leadership. An attribution theory perspective in tourism
Authors: Ali Muhammad , Hassan Muhammad 
Published: January 2023

Purpose
This study aims to examine the mediated effect of ethical leadership between trust based on commitment and compliance-based practices and green behavior intention in tourism sector organizations.

Design/methodology/approach
The existing research in sustainability lacks the effects of leadership types on green management practices. This study uses a quantitative method through survey to determine this effect. Data collection is undertaken on the questionnaire formulated from existing studies. The collected data is analyzed with SmartPLS through measurement and structural model assessment for hypothesis confirmation.

Findings
The findings of the study reveal that ethical leadership does strengthen the relationship between trust and green behavior intention when trust is formed on commitment-based practices in comparison to compliance-based practices.

Research limitations/implications
The study contributes to the attribution theory and its application in sustainability studies by showing that both commitment-based and compliance-based practices help shape the individual trust in an organization which resonates with the assumption that an organization’s seriousness to respond to sustainability issues forms the employee’s trust in the organization. On the practical side, this study provides guidelines for the organizations in tourism industry to demarcate the environmental management activities between compliance and commitment-based practices for better outcomes.

Originality/value
A gap is found in sustainability literature where leadership-type effects are rarely explored in green behavior intention formation. To determine the impact of ethical leadership on the relationship between green management practices and green intention behavior, attribution theory is used as a basis combining social responsibility and tourism organizations. The relationship between green management practices and ethical leadership is a novelty by evaluating the compliance-based and commitment-based practices individually to determine their effect on green behavior intention.
A conjoint analysis of attributes influencing attendance of academic conferences
Authors: Cavusoglu Muhittin , Dogan Seden , Kirant Yozcu Ozen , Hsu Meng-Jun , Cobanoglu Cihan 
Published: January 2023

Purpose
The purpose of this study was to explore the relative importance of various attributes (i.e. the total cost of attending, conference organizer, publishing opportunities for an academic career and personal development, reputation as measured by the number of conferences held, location and size) in terms of their ability to impact the decision of attendees in their selection of which academic conference to attend.

Design/methodology/approach
Conjoint analysis was used to examine the relative importance of various attributes to impact an attendee’s decision to select an academic conference (i.e. the total cost of attending, conference organizer, publishing opportunities for an academic career and personal development, reputation as measured by the number of conferences held, location and size).

Findings
The choice-based conjoint analysis revealed that the most crucial attributes for attending an academic conference were the total cost of attending the conference (28% of relative importance) and the conference organizer (28%).

Practical implications
Since the total cost of attending a conference is one of the top two attributes, conference organizers should reduce the costs as much as possible. Recommendations include choosing a university venue instead of a hotel or conference center and offering multiple options for registration passes (i.e. one-day pass). However, organizers should be careful about meals provided during the conference program, as meals have a positive effect on attendees’ decisions.

Originality/value
This study introduces to the literature a new indicator for selecting an academic conference – the reputation of the conference as measured by the number of times it had been held. In previous studies, this variable was not considered in the context of selecting an academic conference.
Changes in the usefulness of communication channels for prospective undergraduates about university studying: afore and during the pandemic
Authors: Ng Annie W.Y. 
Published: January 2023

PurposeThis study investigated the usefulness of various communication means for providing university study information with prospective students before and during the pandemic for three consecutive academic years.Design/methodology/approachStudents who had recently taken undergraduate program admission indicated how useful communication channels were in providing the information about university studying. Seven communication channels were examined: open/information day, admission talk, campus visit, education fair, admission website, social media and summer activities. The students were asked to complete an online survey at the beginning of an academic year. In the survey, they were asked to indicate the usefulness of each of the communication channels using a five-point Likert scale with the descriptors: 1 = not useful, 2 = slightly useful, 3 = somewhat useful, 4 = useful, 5 = very useful. If students did not have any usage experience with a communication channel, they were asked to choose “no experience.” There were 1798, 1735 and 1961 students at a university in Hong Kong participating in the study in academic years 2019/20, 2020/21 and 2021/22, respectively.FindingsThe pandemic has transformed the landscape of the university's ways of marketing channels to communicate with prospective students. The top useful channel of information for students during the pandemic was admission website, followed by social media and then open/information day. Before the pandemic, open/information day was the most useful channel of information for students and then admission website; the least useful one was social media channel. Significant differences were revealed in the usefulness rating on open/information day and social media channel among the three academic years.Originality/valueThe findings facilitate educational managers to determine the appropriate marketing and recruitment strategies for connecting with prospective students under dynamic context in the era of new normal. They helped identify where to focus the marketing efforts to achieve the most impact in particular under budget constraints.
A moderated-mediated model for examining the impact of fear of pandemic on employees’ turnover intention in Dubai’s hospitality and tourism industry
Authors: AlMutawa Shaikha Ebrahim , Ahmad Kamarul Zaman , Behery Mohamed Hussein , Tabche Ibrahim 
Published: January 2023

Purpose
The global spread of the COVID-19 pandemic has affected businesses worldwide. Arguably, one of the most affected industries is the hospitality sector, where the world has seen a substantial drop in personal and professional travel owing to severe lockdowns, which has particularly harmed the hotel and tourism industries by lowering occupancy and profits. The purpose of this research is to investigate the impact of the COVID-19 epidemic on the emotional and mental well-being of hospitality workers in Dubai, United Arab Emirates.

Design/methodology/approach
A quantitative cross-sectional technique was used, with 517 respondents drawn from Dubai's major hotels.

Findings
Fear of the COVID-19 pandemic was found to be positively connected to employees' emotional weariness, which is then positively related to their intention to leave their organizations.

Practical implications
The paper gives suggestions to managers on how to best deal with their employees, especially the more competent ones, as they are more likely to leave during a pandemic.

Originality/value
Contrary to suggestions in the literature and our predictions, this paper found that self-efficacy positively moderated the relationship between fear of COVID and emotional exhaustion such that workers with high self-efficacy were more prone to emotional exhaustion as a result of their fear of COVID. Furthermore, the results of the research suggest that it is the more competent workers who are more likely to be affected, during a pandemic, and thus their loss is expected to cause greater loss to their organizations.
Impulsiveness in the grocery store: psychographic drivers and segments
Authors: Lord Kenneth R. , Putrevu Sanjay , Olson Elizabeth A. 
Published: January 2023

PurposeThis study aims to enhance the understanding of impulse buying in grocery stores, where such purchases are pervasive and consumers face greater decision fatigue and diminished willpower than in more frequently examined retail environments. The intent is to demonstrate the influence of variables known to affect impulse buying in other environments on grocery shopping behavior, identify and profile segments that vary along those constructs and reveal how those segment characteristics help to explain impulse buying differences.Design/methodology/approachA purposive sample of 234 grocery shoppers approached at the point of purchase in a metropolitan area in Northeastern USA completed scales for theoretically derived variables and reported on their impulse purchases.FindingsAnxiety, perceived financial pressure (PFP), novelty/variety seeking and shopping enjoyment positively influenced, whereas need for cognition had a negative effect on impulse-purchase activity. Two distinct segments of impulse buyers emerged: anxious and innovative shoppers. Anxious shoppers were higher in anxiety, PFP and compulsive buying, whereas innovative shoppers had higher levels of need for cognition and novelty/variety seeking.Originality/valueThe evidence for the dominance of anxiety and novelty/variety seeking as key motivators of distinct segments of impulse buyers in grocery stores is unique to this study. Results yield new insight on tension between the effects of motivational variables on the immediate impulse buying decision and post-purchase evaluation and add precision to marketers’ efforts to encourage spontaneous in-store decision-making.